{"id": "pubmed23n0032_0", "title": "Reliability of plasma cortisol determination by a simple competitive protein binding method.", "content": "The method investigated used (3H) cortisol as the ligand, diluted human plasma as the binding protein, and florisil to separate free and bound cortisol. The precision and specificity were found to be adequate for routine use. The effects of such factors as delay in separation of plasma, storage of plasma, provenance of the binding protein, venepuncture, and venous occlusion have been studied. The extent and significance of short term, inter-daily, and longer term variation in plasma cortisol level are discussed.", "contents": "Reliability of plasma cortisol determination by a simple competitive protein binding method. The method investigated used (3H) cortisol as the ligand, diluted human plasma as the binding protein, and florisil to separate free and bound cortisol. The precision and specificity were found to be adequate for routine use. The effects of such factors as delay in separation of plasma, storage of plasma, provenance of the binding protein, venepuncture, and venous occlusion have been studied. The extent and significance of short term, inter-daily, and longer term variation in plasma cortisol level are discussed.", "PMID": 952472}
{"id": "pubmed23n0032_1", "title": "Accurate measurement of lecithin and sphingomyelin after ultra-rapid chromatographic separation.", "content": "The use of small sandwich chambers containing silica-gel-impregnated glass fibre sheets allows chromatographic separation of lecithin and sphingomyelin in 3 minutes, compared with 45 minutes on conventional silica gel media. Quantitation by measuring the areas of the phospholipid spots after staining with a molybdate stain or 8-anilino-1-naphthylamino sulphonic acid was found to be accurate and more convenient than densitometry. Although slight inaccuracies may be caused by lack of resolution from certain phospholipids, this was not found to be a problem in amniotic fluid analyses as they are either absent or present only in trace quantities. The speed of the technique makes it particularly useful for clinical purposes.", "contents": "Accurate measurement of lecithin and sphingomyelin after ultra-rapid chromatographic separation. The use of small sandwich chambers containing silica-gel-impregnated glass fibre sheets allows chromatographic separation of lecithin and sphingomyelin in 3 minutes, compared with 45 minutes on conventional silica gel media. Quantitation by measuring the areas of the phospholipid spots after staining with a molybdate stain or 8-anilino-1-naphthylamino sulphonic acid was found to be accurate and more convenient than densitometry. Although slight inaccuracies may be caused by lack of resolution from certain phospholipids, this was not found to be a problem in amniotic fluid analyses as they are either absent or present only in trace quantities. The speed of the technique makes it particularly useful for clinical purposes.", "PMID": 952473}
{"id": "pubmed23n0032_2", "title": "An evaluation of the \"CentrifiChem\" parallel fast analyser.", "content": "The operating principles of the CentrifiChem are briefly described and the results of the evaluation are summarised. The pipettor, the temperature control unit, and the photometer were tested separately, but the remainder of the instrument is interdependent. Up to 10 different tests were used to measure within-batch and between-day precision, and accuracy. Carryover was found to be small, and for the six tests investigated the linearity was good. Estimates are given of the running costs, and also included are sections on the reliability and safety of the instrument. Since it requires only a small volume of serum, it is ideally suited to paediatric requirements. When used as an enzyme analyser it can work at about 200 specimens per hour.", "contents": "An evaluation of the \"CentrifiChem\" parallel fast analyser. The operating principles of the CentrifiChem are briefly described and the results of the evaluation are summarised. The pipettor, the temperature control unit, and the photometer were tested separately, but the remainder of the instrument is interdependent. Up to 10 different tests were used to measure within-batch and between-day precision, and accuracy. Carryover was found to be small, and for the six tests investigated the linearity was good. Estimates are given of the running costs, and also included are sections on the reliability and safety of the instrument. Since it requires only a small volume of serum, it is ideally suited to paediatric requirements. When used as an enzyme analyser it can work at about 200 specimens per hour.", "PMID": 952474}
{"id": "pubmed23n0032_3", "title": "How to pass examinations: a personal view on good technique in Clinical Biochemistry examinations.", "content": "Elementary faults in examination technique are commonly displayed by candidates who have worked hard for the examination. Advice is offered on answering multiple-choice questions and \"essay\" questions and on how to tackle oral and practical examinations. A candidate's preparation for an examination should include the submission of written work and the taking of a mock oral examination for critical assessment by a supervisor. Careful writing up of practical work in the home laboratory is important, so that faulty technique can be corrected early in training. Above all, stress is laid on the importance of mastering the technique of effective communication for success both in examinations and in later professional life.", "contents": "How to pass examinations: a personal view on good technique in Clinical Biochemistry examinations. Elementary faults in examination technique are commonly displayed by candidates who have worked hard for the examination. Advice is offered on answering multiple-choice questions and \"essay\" questions and on how to tackle oral and practical examinations. A candidate's preparation for an examination should include the submission of written work and the taking of a mock oral examination for critical assessment by a supervisor. Careful writing up of practical work in the home laboratory is important, so that faulty technique can be corrected early in training. Above all, stress is laid on the importance of mastering the technique of effective communication for success both in examinations and in later professional life.", "PMID": 952475}
{"id": "pubmed23n0032_4", "title": "Comparison of a gas chromatographic and colorimetric method for the determination of plasma paracetamol.", "content": "Plasma paracetamol levels have generally been determined either by gas chromatography (Stewart and Willis, 1975), which relies on complex equipment, or by spectrophotometry (Knepil, 1974), which can be time-consuming. The introduction by Glynn and Kendal (1975) of a simple colorimetric method based on the reaction of paracetamol with nitrous acid to give 2-nitro-4-acetamidophenol appears to have overcome these disadvantages, thereby providing a suitable procedure for the rapid measurement of plasma paracetamol in cases of overdose. The method was reported to be specific for paracetamol, no interference being caused either by the sulphate and glucuronide conjugates of paracetamol or by a large number of other commonly found drugs. This communication presents the results of a study in which plasma paracetamol levels determined by the colorimetric method were compared with those determined by an established gas chromatographic technique.", "contents": "Comparison of a gas chromatographic and colorimetric method for the determination of plasma paracetamol. Plasma paracetamol levels have generally been determined either by gas chromatography (Stewart and Willis, 1975), which relies on complex equipment, or by spectrophotometry (Knepil, 1974), which can be time-consuming. The introduction by Glynn and Kendal (1975) of a simple colorimetric method based on the reaction of paracetamol with nitrous acid to give 2-nitro-4-acetamidophenol appears to have overcome these disadvantages, thereby providing a suitable procedure for the rapid measurement of plasma paracetamol in cases of overdose. The method was reported to be specific for paracetamol, no interference being caused either by the sulphate and glucuronide conjugates of paracetamol or by a large number of other commonly found drugs. This communication presents the results of a study in which plasma paracetamol levels determined by the colorimetric method were compared with those determined by an established gas chromatographic technique.", "PMID": 952476}
{"id": "pubmed23n0032_5", "title": "A study of factors influencing continuous flow kinetics: the use of serum calcium estimation as a model.", "content": "Previous studies of continuous flow kinetics have described an \"AutoAnalyzer\" peak in terms of two time constants, lag constant \"a\" and exponential constant \"b\". Further investigations described here define some of the factors which affect these constants and hence peak shape and analytical performance. This communication outlines the application of this information to the development of an automated method for the determination of serum calcium, having a carry-over of less than 1% and an analytical rate greater than 100/hour. The instaneous colour reaction, simple hydraulics, removal of the dialysis step, and the use of an integral debubbling flow cell have enabled small kinetic constants to be achieved. Over a period of three months' routine use between-batch co-efficient of variation (C.V.) was 1.4%.", "contents": "A study of factors influencing continuous flow kinetics: the use of serum calcium estimation as a model. Previous studies of continuous flow kinetics have described an \"AutoAnalyzer\" peak in terms of two time constants, lag constant \"a\" and exponential constant \"b\". Further investigations described here define some of the factors which affect these constants and hence peak shape and analytical performance. This communication outlines the application of this information to the development of an automated method for the determination of serum calcium, having a carry-over of less than 1% and an analytical rate greater than 100/hour. The instaneous colour reaction, simple hydraulics, removal of the dialysis step, and the use of an integral debubbling flow cell have enabled small kinetic constants to be achieved. Over a period of three months' routine use between-batch co-efficient of variation (C.V.) was 1.4%.", "PMID": 952478}
{"id": "pubmed23n0032_6", "title": "Evaluation of a serum/plasma separator: suitability of samples for radioimmunoassay and competitive protein binding techniques.", "content": "A new method of separating serum or plasma from whole blood (Sure-Sep, W.R. Warner) has been evaluated with particular regard to the suitability of the serum/plasma samples for radioimmunoassay (R.I.A.) and competitive protein binding (C.P.B.) techniques. Sure-Sep did not affect the results obtained for the following assays: cortisol, thyroxine, triidothyronine, insulin, and growth hormone. In addition it was also found that contact of the serum or plasma samples with Sure-Sep for 24 hours at 4 degrees C was without effect on the results obtained.", "contents": "Evaluation of a serum/plasma separator: suitability of samples for radioimmunoassay and competitive protein binding techniques. A new method of separating serum or plasma from whole blood (Sure-Sep, W.R. Warner) has been evaluated with particular regard to the suitability of the serum/plasma samples for radioimmunoassay (R.I.A.) and competitive protein binding (C.P.B.) techniques. Sure-Sep did not affect the results obtained for the following assays: cortisol, thyroxine, triidothyronine, insulin, and growth hormone. In addition it was also found that contact of the serum or plasma samples with Sure-Sep for 24 hours at 4 degrees C was without effect on the results obtained.", "PMID": 952479}
{"id": "pubmed23n0032_7", "title": "The peak of the likelihood curve in linkage testing.", "content": "It is often suggested that when z-scores are used for linkage estimation, a maximum z-score of 3 (or maximum antilod score, M = 1000) is good evidence for the presence of linkage. It is pointed out that a better formula, applicable if nothing more is known about the loci concerned, would be that the probability that linkage is present is roughly 1--4/Msigma, whereas sigma is the standard error, i.e. square root(2-3 X downward curvature of z curve at peak). A consideration of typical cases suggests that a value M = 1000 would imply something like a 90% chance of linkage. It would seem simpler to use the formula, probability of linkage approximately to 1--H/20, where H is the average height of the antilod curve.", "contents": "The peak of the likelihood curve in linkage testing. It is often suggested that when z-scores are used for linkage estimation, a maximum z-score of 3 (or maximum antilod score, M = 1000) is good evidence for the presence of linkage. It is pointed out that a better formula, applicable if nothing more is known about the loci concerned, would be that the probability that linkage is present is roughly 1--4/Msigma, whereas sigma is the standard error, i.e. square root(2-3 X downward curvature of z curve at peak). A consideration of typical cases suggests that a value M = 1000 would imply something like a 90% chance of linkage. It would seem simpler to use the formula, probability of linkage approximately to 1--H/20, where H is the average height of the antilod curve.", "PMID": 952482}
{"id": "pubmed23n0032_8", "title": "On the estimation of parental age effects on mutation.", "content": "The detection and estimation of the effects of paternal and maternal age and birth rank on mutation rate are considered. Smith's (1972) method and discriminant function techniques are compared using data on Apert's acrocephalosyndactyly and achondroplasia.", "contents": "On the estimation of parental age effects on mutation. The detection and estimation of the effects of paternal and maternal age and birth rank on mutation rate are considered. Smith's (1972) method and discriminant function techniques are compared using data on Apert's acrocephalosyndactyly and achondroplasia.", "PMID": 952483}
{"id": "pubmed23n0032_9", "title": "A note on the arrival probability, first arrival time and age of a mutant gene in a finite population.", "content": "It is pointed out that the arrival or fixation probability of a mutant gene can be easily inferred analytically. The mean first arrival time for a single overdominant mutation to reach frequency y attains its maximum when x is close to but still slightly less than y/2, where x is the equilibrium frequency of the mutant gene in an infinitely large population. For an advantageous mutation, the mean first arrival time decreases with an increasing degree of dominance if selection is strong, but it first increases, after reaching a maximum, then decreases as the degree of dominance increases, if selection is weak. Contrary to our intuition, the mean age of an advantageous mutant gene increases with increasing degree of dominance, except when selection is very strong. A simple explanation is given in terms of the sojourn time at a particular gene frequency.", "contents": "A note on the arrival probability, first arrival time and age of a mutant gene in a finite population. It is pointed out that the arrival or fixation probability of a mutant gene can be easily inferred analytically. The mean first arrival time for a single overdominant mutation to reach frequency y attains its maximum when x is close to but still slightly less than y/2, where x is the equilibrium frequency of the mutant gene in an infinitely large population. For an advantageous mutation, the mean first arrival time decreases with an increasing degree of dominance if selection is strong, but it first increases, after reaching a maximum, then decreases as the degree of dominance increases, if selection is weak. Contrary to our intuition, the mean age of an advantageous mutant gene increases with increasing degree of dominance, except when selection is very strong. A simple explanation is given in terms of the sojourn time at a particular gene frequency.", "PMID": 952485}
{"id": "pubmed23n0032_10", "title": "Growth of deleterious mutant genes in a large population.", "content": "Formulae for the arrival probability and first arrival time for a single mutant gene to reach a certain number have been obtained by using a continuous branching process. If the mean of the progeny number of heterozygotes is less than one the arrival probability increases with increasing variance of the progeny distribution whereas if the mean is greater than one the contrary is true. Since most human populations are growing at fairly high rates, the result indicates that the probability for a single mutation to grow to a large number is quite high. Numerical computations show that the mean first arrival time decreases with increasing variance of the progeny distribution of the mutant carriers. The results have been applied to investigate the case of the acheiropodia gene in Brazil. The hypothesis that all acheiropodia genes in Brazil were derived from a single mutation seems to be tenable.", "contents": "Growth of deleterious mutant genes in a large population. Formulae for the arrival probability and first arrival time for a single mutant gene to reach a certain number have been obtained by using a continuous branching process. If the mean of the progeny number of heterozygotes is less than one the arrival probability increases with increasing variance of the progeny distribution whereas if the mean is greater than one the contrary is true. Since most human populations are growing at fairly high rates, the result indicates that the probability for a single mutation to grow to a large number is quite high. Numerical computations show that the mean first arrival time decreases with increasing variance of the progeny distribution of the mutant carriers. The results have been applied to investigate the case of the acheiropodia gene in Brazil. The hypothesis that all acheiropodia genes in Brazil were derived from a single mutation seems to be tenable.", "PMID": 952486}
{"id": "pubmed23n0032_11", "title": "Dominance variance in Fisher's model of assortative mating.", "content": "It is argued that there is some confusion in the literature concerning the effect of assortative mating on dominance variance. It is shown that in Fisher's model of assortative mating additive deviations of one factor are correlated with additive deviations of another and that this correlation is responsible for the increase in genetic variance. The dominance deviations of one factor are independent of the dominance, as well as of the additive deviations of a second factor. It is shown that in Fisher's model of assortative mating and to the approximation used by him, the dominance variance does not change under assortative mating.", "contents": "Dominance variance in Fisher's model of assortative mating. It is argued that there is some confusion in the literature concerning the effect of assortative mating on dominance variance. It is shown that in Fisher's model of assortative mating additive deviations of one factor are correlated with additive deviations of another and that this correlation is responsible for the increase in genetic variance. The dominance deviations of one factor are independent of the dominance, as well as of the additive deviations of a second factor. It is shown that in Fisher's model of assortative mating and to the approximation used by him, the dominance variance does not change under assortative mating.", "PMID": 952487}
{"id": "pubmed23n0032_12", "title": "A model for assortative mating.", "content": "It is shown that a generalization of the Hardy-Weinberg law of the proportions of genotypes of the kind given by Wright (1922) is maintained in equilibrium by a pattern of assortative mating suggested by Fisher (1918) and elaborated by Mal\u00e9cot (1939, 1948).", "contents": "A model for assortative mating. It is shown that a generalization of the Hardy-Weinberg law of the proportions of genotypes of the kind given by Wright (1922) is maintained in equilibrium by a pattern of assortative mating suggested by Fisher (1918) and elaborated by Mal\u00e9cot (1939, 1948).", "PMID": 952488}
{"id": "pubmed23n0032_13", "title": "On the method of Penrose of estimating the number of effective factors contributing to a character.", "content": "Penrose (1969) presented a method of estimating the number of effective factors contributing to a quantitative character. The method uses samples of parent-child and sib-sib phenotypic values. More detailed mathematical analysis shows that his assertions regarding the method are correct under the assumptions which he made, provided that his phrase 'the same quantitative effect' is interpreted to mean 'makes an equal contribution to the variance'.", "contents": "On the method of Penrose of estimating the number of effective factors contributing to a character. Penrose (1969) presented a method of estimating the number of effective factors contributing to a quantitative character. The method uses samples of parent-child and sib-sib phenotypic values. More detailed mathematical analysis shows that his assertions regarding the method are correct under the assumptions which he made, provided that his phrase 'the same quantitative effect' is interpreted to mean 'makes an equal contribution to the variance'.", "PMID": 952489}
{"id": "pubmed23n0032_14", "title": "A genetic study on the length of tibia.", "content": "The length of tibia was studied in 1364 males and 1791 females over 10 years of age in an agricultural district of Wakayama Prefecture, Japan. (1) The length markedly increased from 10 to 17 years of age in males, and 10 to 15 in females, but it remained comparatively unchanged from 20 to 79 years in both sexes. The following regressions of tibial length covering 20 to 79 years were obtained: Y male = 0-0183x+34-7892, Y female = -0-0269x + 32-5329. The modified data proved to be a normal distribution on skewness and kurtosis test; they were analysed on a quantitative genetic basis. (2) No significant contribution of X-linked and Y-linked genes to tibial length was found in an analysis of parent-child combinations. (3) There was no indication of maternal effect, since the difference between father-child (r = 0-36 +/- 0-05) and mother-child (r = 0-42 +/- 0-04) correlations was not significant. (4) Correlation coefficients between sibs were significant, the values being 0-57 +/- 0-09 for brother-brother, 0-32 +/- 0-22 for sister-sister, and 0-27 +/- 0-13 for brother-sister. Correlation coeffiecients between parent and child were also significant, the values being 0-36 +/- 0-06 for father-son, 0-35 +/- 0-09 for father-daughter, 0-44 +/- 0-04 for mother-son, 0-35 +/- 0-07 for mother-daughter. The regression coefficients of child on midparent were calculated to be 0-77 +/- 0-12. Thus, the heritability for the length of tibia may be estimated to be 0-77 approximately 0-80. Contribution of dominance variance to total variance was very small (VD = 0-01), in contrast to a far greater additive genetic variance (VA = 0-80).", "contents": "A genetic study on the length of tibia. The length of tibia was studied in 1364 males and 1791 females over 10 years of age in an agricultural district of Wakayama Prefecture, Japan. (1) The length markedly increased from 10 to 17 years of age in males, and 10 to 15 in females, but it remained comparatively unchanged from 20 to 79 years in both sexes. The following regressions of tibial length covering 20 to 79 years were obtained: Y male = 0-0183x+34-7892, Y female = -0-0269x + 32-5329. The modified data proved to be a normal distribution on skewness and kurtosis test; they were analysed on a quantitative genetic basis. (2) No significant contribution of X-linked and Y-linked genes to tibial length was found in an analysis of parent-child combinations. (3) There was no indication of maternal effect, since the difference between father-child (r = 0-36 +/- 0-05) and mother-child (r = 0-42 +/- 0-04) correlations was not significant. (4) Correlation coefficients between sibs were significant, the values being 0-57 +/- 0-09 for brother-brother, 0-32 +/- 0-22 for sister-sister, and 0-27 +/- 0-13 for brother-sister. Correlation coeffiecients between parent and child were also significant, the values being 0-36 +/- 0-06 for father-son, 0-35 +/- 0-09 for father-daughter, 0-44 +/- 0-04 for mother-son, 0-35 +/- 0-07 for mother-daughter. The regression coefficients of child on midparent were calculated to be 0-77 +/- 0-12. Thus, the heritability for the length of tibia may be estimated to be 0-77 approximately 0-80. Contribution of dominance variance to total variance was very small (VD = 0-01), in contrast to a far greater additive genetic variance (VA = 0-80).", "PMID": 952491}
{"id": "pubmed23n0032_15", "title": "Extensions to pedigree analysis. III. Variance components by the scoring method.", "content": "The classic variance components for simple polygenic traits - additive, dominance, and environmental variance - have traditionally been estimated from sample covariances between first-degree relatives. If data is gathered on pedigrees, this statistical procedure wastes information. Recently Elston & Stewart suggested an alternative likelihood procedure that uses all the information in a set of pedigrees. A refinement of their method based on the scoring technique gives rapidly converging maximum likelihood estimates of the variance components and of the male and female means. Tests of statistical hypotheses about the various parameters can then be made by the likelihood ratio method. Furthermore, using classical regression analysis, the estimated parameter values allow prediction of unknown trait values from known trait values within a pedigree. These methods should apply to traits like total finger ridge count and to quantitative measurements associated with disease traits. Since the model postulates independent environmental effects and no assortative mating, its utility in human behaviour genetics seems limited.", "contents": "Extensions to pedigree analysis. III. Variance components by the scoring method. The classic variance components for simple polygenic traits - additive, dominance, and environmental variance - have traditionally been estimated from sample covariances between first-degree relatives. If data is gathered on pedigrees, this statistical procedure wastes information. Recently Elston & Stewart suggested an alternative likelihood procedure that uses all the information in a set of pedigrees. A refinement of their method based on the scoring technique gives rapidly converging maximum likelihood estimates of the variance components and of the male and female means. Tests of statistical hypotheses about the various parameters can then be made by the likelihood ratio method. Furthermore, using classical regression analysis, the estimated parameter values allow prediction of unknown trait values from known trait values within a pedigree. These methods should apply to traits like total finger ridge count and to quantitative measurements associated with disease traits. Since the model postulates independent environmental effects and no assortative mating, its utility in human behaviour genetics seems limited.", "PMID": 952492}
{"id": "pubmed23n0032_16", "title": "The genotypic distribution of relatives of homozygotes when consanguinity is present.", "content": "A correction is made to an expression given by Jacquard (1968, 1972a,b) for the genotypic distribution of a relative of a given homozygote when consanguinity is present. The correction will necessarily be small either if the allele present in the given homozygote has a frequency of near unity, or if the coefficient of inbreeding of the given homozygote is small.", "contents": "The genotypic distribution of relatives of homozygotes when consanguinity is present. A correction is made to an expression given by Jacquard (1968, 1972a,b) for the genotypic distribution of a relative of a given homozygote when consanguinity is present. The correction will necessarily be small either if the allele present in the given homozygote has a frequency of near unity, or if the coefficient of inbreeding of the given homozygote is small.", "PMID": 952493}
{"id": "pubmed23n0032_17", "title": "Isonymy in a Bhatia leut.", "content": "Isonymy has been described in a North Indian Hindu Community, which shows surname exogamy. A modification is suggested in the formula of Crow and Mange for the estimation of FIS to make it applicable to populations exhibiting clan exogamy. The values of FIS obtained by different methods are compared.", "contents": "Isonymy in a Bhatia leut. Isonymy has been described in a North Indian Hindu Community, which shows surname exogamy. A modification is suggested in the formula of Crow and Mange for the estimation of FIS to make it applicable to populations exhibiting clan exogamy. The values of FIS obtained by different methods are compared.", "PMID": 952494}
{"id": "pubmed23n0032_18", "title": "[Assay of the activity of horseradish peroxidase antibodies using ammonium sulfate technique (author's transl)].", "content": "The antigen binding capacity (ABC) of mouse and rabbit antisera to horseradish peroxidase (HRP) was measured by the Farr technique, originally introduced for bovine serum albumin. Results obtained by this method are independent of other antibody interactions (precipitation, formation of immune complexes, effect on enzyme activity). Commercially available HRP has variable solubility in ammonium sulfate solutions; almost 40% was precipitated at 50% saturation. Accordingly, the fraction of HRP soluble at 60% saturation was used, and this was labelled with 125I2 using the HRP oxidizing activity. Under these test conditions, accurate and reproducible results were obtained with protein concentrations (as N/ml) of the order to 0.07 mug (antibody) and 0.01 mug (antigen) respectively. This high sensitivity is superior to that achieved by passive hemagglutination, especially when titrating antibody levels in mouse sera.", "contents": "[Assay of the activity of horseradish peroxidase antibodies using ammonium sulfate technique (author's transl)]. The antigen binding capacity (ABC) of mouse and rabbit antisera to horseradish peroxidase (HRP) was measured by the Farr technique, originally introduced for bovine serum albumin. Results obtained by this method are independent of other antibody interactions (precipitation, formation of immune complexes, effect on enzyme activity). Commercially available HRP has variable solubility in ammonium sulfate solutions; almost 40% was precipitated at 50% saturation. Accordingly, the fraction of HRP soluble at 60% saturation was used, and this was labelled with 125I2 using the HRP oxidizing activity. Under these test conditions, accurate and reproducible results were obtained with protein concentrations (as N/ml) of the order to 0.07 mug (antibody) and 0.01 mug (antigen) respectively. This high sensitivity is superior to that achieved by passive hemagglutination, especially when titrating antibody levels in mouse sera.", "PMID": 952496}
{"id": "pubmed23n0032_19", "title": "[The influence of plasmocellular tumours on the humoral immune response in mice (author's transl)].", "content": "Syngeneic mice of the C3H/He strain have been inoculated with cells of the plasmocellular tumours X5563 and X5647, producing myeloma proteins of the IgG and IgA class respectively, and were immunized with (1) sheep red blood cells, (2) bovine serum albumin and (3) horseradish peroxydase. The immune responses of the various groups of mice were followed. The antibody activities towards all three antigens were decreased in mice carrying the IgG producing tumour X5563. This effect was correlated with an enhanced catabolism of IgG as well as with a decrease in number of IgG antibody producing cells. A reduced immune response was also observed in mice to which the IgA producing tumour X5647 had been transferred, but the effect was significantly less pronounced. There was no effect at all observed on the IgM response. The avidity which was measured for the various IgG antibodies was not modified by the presence of IgG nor IgA secreting myeloma tumours.", "contents": "[The influence of plasmocellular tumours on the humoral immune response in mice (author's transl)]. Syngeneic mice of the C3H/He strain have been inoculated with cells of the plasmocellular tumours X5563 and X5647, producing myeloma proteins of the IgG and IgA class respectively, and were immunized with (1) sheep red blood cells, (2) bovine serum albumin and (3) horseradish peroxydase. The immune responses of the various groups of mice were followed. The antibody activities towards all three antigens were decreased in mice carrying the IgG producing tumour X5563. This effect was correlated with an enhanced catabolism of IgG as well as with a decrease in number of IgG antibody producing cells. A reduced immune response was also observed in mice to which the IgA producing tumour X5647 had been transferred, but the effect was significantly less pronounced. There was no effect at all observed on the IgM response. The avidity which was measured for the various IgG antibodies was not modified by the presence of IgG nor IgA secreting myeloma tumours.", "PMID": 952495}
{"id": "pubmed23n0032_20", "title": "[Delayed-type hypersensitivity induced by digestive immunization and suppression of this induction by antigen-antibody complexes (author's transl)].", "content": "BALB/c mice were immunized by intragastric administration of sheep red blood cells or horse red blood cells repeated daily for 4 days. The immunized animals when skin tested with an urea extract of the corresponding antigen showed a delayed-type hypersensitivity reaction. This reaction was observed for 4 weeks after the immunization. This result demonstrates that digestive immunization may induce cellular immunity. When serum from intragastrically immunised mice containing antigen-antibody complexes was administered intraperitoneally 8 hours before the onset of digestive immunization, the subsequent delayed-type hypersensitivity reaction was suppressed or considerably depressed.", "contents": "[Delayed-type hypersensitivity induced by digestive immunization and suppression of this induction by antigen-antibody complexes (author's transl)]. BALB/c mice were immunized by intragastric administration of sheep red blood cells or horse red blood cells repeated daily for 4 days. The immunized animals when skin tested with an urea extract of the corresponding antigen showed a delayed-type hypersensitivity reaction. This reaction was observed for 4 weeks after the immunization. This result demonstrates that digestive immunization may induce cellular immunity. When serum from intragastrically immunised mice containing antigen-antibody complexes was administered intraperitoneally 8 hours before the onset of digestive immunization, the subsequent delayed-type hypersensitivity reaction was suppressed or considerably depressed.", "PMID": 952497}
{"id": "pubmed23n0032_21", "title": "[Actinomyces globispororoseus var. granaticus var. nov., a new producer of granaticins].", "content": "Actinomycete LIA-0830 was most close by its morphological and cultural features tc Act. globispororoseus. Jet it differed from the latter in the physiological and antibioti properties. Actinomycete LIA-0830 differed from the known granaticin- and granaticin B-producing organisms by its morphological, cultural and physiological properties. On the basis of these data actinomycete LIA-0830 was classified as Actinomyces globispororoseus var. granaticus var. nov. The culture produced 2 antibiotics: granaticin and granaticin B.", "contents": "[Actinomyces globispororoseus var. granaticus var. nov., a new producer of granaticins]. Actinomycete LIA-0830 was most close by its morphological and cultural features tc Act. globispororoseus. Jet it differed from the latter in the physiological and antibioti properties. Actinomycete LIA-0830 differed from the known granaticin- and granaticin B-producing organisms by its morphological, cultural and physiological properties. On the basis of these data actinomycete LIA-0830 was classified as Actinomyces globispororoseus var. granaticus var. nov. The culture produced 2 antibiotics: granaticin and granaticin B.", "PMID": 952500}
{"id": "pubmed23n0032_22", "title": "[Production of tubular structures from the aerial mycelium of Actinomyces roseoflavus var. roseofungini].", "content": "Tubular formations analogous by their structure to those found on the surface of the outer sheath of the hyphae of the aerial mycelium of Actinomyces roseoflavus var. roseofungini were reconstructed in vitro from the mycelium acetone extract on addition of water. A polyenic antibiotic was shown to be present in the composition of the \"tubes\". A possible role of the antibiotic in the genesis of the secondary structures of the actinomycete is discussed.", "contents": "[Production of tubular structures from the aerial mycelium of Actinomyces roseoflavus var. roseofungini]. Tubular formations analogous by their structure to those found on the surface of the outer sheath of the hyphae of the aerial mycelium of Actinomyces roseoflavus var. roseofungini were reconstructed in vitro from the mycelium acetone extract on addition of water. A polyenic antibiotic was shown to be present in the composition of the \"tubes\". A possible role of the antibiotic in the genesis of the secondary structures of the actinomycete is discussed.", "PMID": 952501}
{"id": "pubmed23n0032_23", "title": "Further characterization of milk pyroglobulin.", "content": "Milk pyroglobulin was capable of passing through the \"Visking\" 8/32 cellulose membrane, when dialysed against deionized water but not against saline. Gel filtration analysis of the protein suggested its dissociation into subunit molecules in deionized water. Amino acid composition revealed high contents of proline, glutamic acid, valine and leucine. The protein possessed a biological activity to increase the permeability of skin vessels when injected intradermally. The colostral whey which was experimentally freed from heat-coagulable substances contained as major components proteins which are antigenically related to the pyroglobulin.", "contents": "Further characterization of milk pyroglobulin. Milk pyroglobulin was capable of passing through the \"Visking\" 8/32 cellulose membrane, when dialysed against deionized water but not against saline. Gel filtration analysis of the protein suggested its dissociation into subunit molecules in deionized water. Amino acid composition revealed high contents of proline, glutamic acid, valine and leucine. The protein possessed a biological activity to increase the permeability of skin vessels when injected intradermally. The colostral whey which was experimentally freed from heat-coagulable substances contained as major components proteins which are antigenically related to the pyroglobulin.", "PMID": 952498}
{"id": "pubmed23n0032_24", "title": "[Teichoic acid content in the cell wall of Actinomyces rimosus LST-118 in the process of ontogeny].", "content": "Polymeric compounds of the cell walls of Act. rimosus were studied during the actinomycete growth. It was found that the content of teichoic acid decreased 2 times by the end of development of Act. rimosus, while content of glycane polymer remained approximately at the same level. The molar ratios of teichoic acid and glycopeptide glycane were determined.", "contents": "[Teichoic acid content in the cell wall of Actinomyces rimosus LST-118 in the process of ontogeny]. Polymeric compounds of the cell walls of Act. rimosus were studied during the actinomycete growth. It was found that the content of teichoic acid decreased 2 times by the end of development of Act. rimosus, while content of glycane polymer remained approximately at the same level. The molar ratios of teichoic acid and glycopeptide glycane were determined.", "PMID": 952502}
{"id": "pubmed23n0032_25", "title": "[Prevalence of markers characteristic for penicillinase plasmids in clinical strains of straphylococci].", "content": "Occurrence of the markers of penicillin and erythromycin resistance in the clinical strains of Staph. aureus, as well as their connection with the determinants of resistance to the heavy metal salts and sodium arsenate was studied. 79 per cent of Staph. aureus were resistant to penicillin and 47 per cent to erythromycin. All erythromycin resistant strains were also resistant to penicillin. More than a half of the strains of Staph. aureus of the phage groups I, III and the mixed phage group had a set of markers: pen, asa, cad, mer, ego. The rate of elimination of the above markers with ethidium bromide was high. The presence of the penicillinase plasmid determining a rather low level of resistance to erythromycin (20--50 gamma/ml) was characteristic of the strains belonging to the phage group I. The presence of the plasmid determining a high level of resistance to that drug (500--1000 gamma/ml) was characteristic of the phage group III and the mixed phage group. The erythromycin sensitive strains of Staph. aureus were almost always sensitive to mercuric ions.", "contents": "[Prevalence of markers characteristic for penicillinase plasmids in clinical strains of straphylococci]. Occurrence of the markers of penicillin and erythromycin resistance in the clinical strains of Staph. aureus, as well as their connection with the determinants of resistance to the heavy metal salts and sodium arsenate was studied. 79 per cent of Staph. aureus were resistant to penicillin and 47 per cent to erythromycin. All erythromycin resistant strains were also resistant to penicillin. More than a half of the strains of Staph. aureus of the phage groups I, III and the mixed phage group had a set of markers: pen, asa, cad, mer, ego. The rate of elimination of the above markers with ethidium bromide was high. The presence of the penicillinase plasmid determining a rather low level of resistance to erythromycin (20--50 gamma/ml) was characteristic of the strains belonging to the phage group I. The presence of the plasmid determining a high level of resistance to that drug (500--1000 gamma/ml) was characteristic of the phage group III and the mixed phage group. The erythromycin sensitive strains of Staph. aureus were almost always sensitive to mercuric ions.", "PMID": 952503}
{"id": "pubmed23n0032_26", "title": "[Effect of glucocorticoids on the pharmacokinetics of kanamycin in rabbits].", "content": "The effect of hydrocortisone on kanamycin pharmacokinetics in rabbits treated with the drugs administered intramuscularly in two different syringes simultaneously and in one syringe was studied. When the drugs were administered in two different syringes, there were no changes in the antibiotic pharmacokinetics as compared to the control group, while administration of the drugs in one syringe resulted in decreased excretion of the antibiotic with urine and a significant increase of its levels in the blood, liquor and tissues.", "contents": "[Effect of glucocorticoids on the pharmacokinetics of kanamycin in rabbits]. The effect of hydrocortisone on kanamycin pharmacokinetics in rabbits treated with the drugs administered intramuscularly in two different syringes simultaneously and in one syringe was studied. When the drugs were administered in two different syringes, there were no changes in the antibiotic pharmacokinetics as compared to the control group, while administration of the drugs in one syringe resulted in decreased excretion of the antibiotic with urine and a significant increase of its levels in the blood, liquor and tissues.", "PMID": 952504}
{"id": "pubmed23n0032_27", "title": "[Absorption, distribution and the duration of retention of neomycin in the body of pregnant rabbits and their fetuses].", "content": "Neomycin was administered intramuscularly to rabbits in single doses of 50000 gamma/kg 24--25-day gravid rabbits were sacrificed 1.5-2-3-4-6-12-24 hours and 2-3-6 days after the drug administration. 3--4 rabbits were used in every experiment. The antibiotic concentration was determined with the agar-diffusion method. It was found that the drug was present in various bactericidal concentrations in all 55 organs, tissues and fluids of the rabbits and the fetus examined except the crystalline lens in the rabbits and the brain, liver and bile cyst of the fetus. In some material of the rabbits and their fetus, high levels of neomycin were detected during 6 days, i. e. up to the end of the experiment. The highest neomycin levels in the organs, tissues and fluids of the fetus were detected somewhat later than in those of the rabbits. However, their retention time was longer. It is necessary to forbear from using antibiotics, such as neomycin, monomycin and others, which retained for long periods of time in the organism.", "contents": "[Absorption, distribution and the duration of retention of neomycin in the body of pregnant rabbits and their fetuses]. Neomycin was administered intramuscularly to rabbits in single doses of 50000 gamma/kg 24--25-day gravid rabbits were sacrificed 1.5-2-3-4-6-12-24 hours and 2-3-6 days after the drug administration. 3--4 rabbits were used in every experiment. The antibiotic concentration was determined with the agar-diffusion method. It was found that the drug was present in various bactericidal concentrations in all 55 organs, tissues and fluids of the rabbits and the fetus examined except the crystalline lens in the rabbits and the brain, liver and bile cyst of the fetus. In some material of the rabbits and their fetus, high levels of neomycin were detected during 6 days, i. e. up to the end of the experiment. The highest neomycin levels in the organs, tissues and fluids of the fetus were detected somewhat later than in those of the rabbits. However, their retention time was longer. It is necessary to forbear from using antibiotics, such as neomycin, monomycin and others, which retained for long periods of time in the organism.", "PMID": 952505}
{"id": "pubmed23n0032_28", "title": "[Functional state of the adrenal cortex in the antibiotic therapy of experimental staphylococcal infection].", "content": "The functional state of the cortex in experimental animals before and after treatment with tetracycline was studied under conditions of prolonged infection caused by intravenous administration of Staph. aureus. Three staphylococcal strains were used in the study: sensitive, low sensitive and resistant to tetracycline. It was shown that development of the infection in the animals was accompanied by a decrease in the cortex function. Oral administration of tetracycline in a dose of 200 mg/kg resulted in normalization of the glucocorticoid function of the gland after 15 days of its use. Since the same phenomenon was observed when the animals were infected with the antibiotic resistant strains of staphylococci, it was supposed that the therapeutic effect of tetracycline was defined by both the antibacterial action and capacity for increasing the protective-adaptive reactions of the host by stimulation of the cortex function.", "contents": "[Functional state of the adrenal cortex in the antibiotic therapy of experimental staphylococcal infection]. The functional state of the cortex in experimental animals before and after treatment with tetracycline was studied under conditions of prolonged infection caused by intravenous administration of Staph. aureus. Three staphylococcal strains were used in the study: sensitive, low sensitive and resistant to tetracycline. It was shown that development of the infection in the animals was accompanied by a decrease in the cortex function. Oral administration of tetracycline in a dose of 200 mg/kg resulted in normalization of the glucocorticoid function of the gland after 15 days of its use. Since the same phenomenon was observed when the animals were infected with the antibiotic resistant strains of staphylococci, it was supposed that the therapeutic effect of tetracycline was defined by both the antibacterial action and capacity for increasing the protective-adaptive reactions of the host by stimulation of the cortex function.", "PMID": 952506}
{"id": "pubmed23n0032_29", "title": "[Effect of the prolonged use of streptomycin as well as streptomycin in combination with tubazid on the bioelectrical activity of the brain in experimental tuberculosis].", "content": "The effect of streptomycin used alone and in combination with tubazid on the brain electric activity was studied in chronic experiments on rabbits with tuberculosis. The electrocorticographical investigations showed that the antibacterial treatment lowered the spontaneous electric activity and reactive capacity of the cortex. It was evident from a narrow amplitude of the main rythm, coefficient and energy of synchronization on rythmic photostimulation and paroxysmal activity withing the theta range. With the account of the high therapeutic effect of the antibacterial treatment evident from minimum affection of the organs with tuberculosis, the above changes in the electrocorticogrammes should be attributed to the neurotropic effect of the drug and not to tuberculosis intoxication.", "contents": "[Effect of the prolonged use of streptomycin as well as streptomycin in combination with tubazid on the bioelectrical activity of the brain in experimental tuberculosis]. The effect of streptomycin used alone and in combination with tubazid on the brain electric activity was studied in chronic experiments on rabbits with tuberculosis. The electrocorticographical investigations showed that the antibacterial treatment lowered the spontaneous electric activity and reactive capacity of the cortex. It was evident from a narrow amplitude of the main rythm, coefficient and energy of synchronization on rythmic photostimulation and paroxysmal activity withing the theta range. With the account of the high therapeutic effect of the antibacterial treatment evident from minimum affection of the organs with tuberculosis, the above changes in the electrocorticogrammes should be attributed to the neurotropic effect of the drug and not to tuberculosis intoxication.", "PMID": 952507}
{"id": "pubmed23n0032_30", "title": "[Antiviral activity of a poly A-poly U complex administered by various routes].", "content": "Interferon-inducing and antiviral activity of a synthetic polyribonucleotide, polyaenylic and polyuridylic acid complex (poly A--poly U) was studied comparatively on a model of experimental infection of albino mice caused by Venezuela Horse Encephalomyeliti Viruos (VHEV) using oral and intraperitoneal administration of the complex. It was shown that dm-interferon induction and antiviral effect comparatively high doses of poly A--poly U (Reanal) were required (1 mg/mouse). The highest antiviral effect was observed after a two-fold administration of the drug (24 hours and immediately before infection). It was more evident after intraperitoneal administration of the inductor. The interferon titers in the animal blood serum after intraperitoneal or oral administration of poly A--poly U were almost the same 58=60+/- +/- 18.9 IU50/ml).", "contents": "[Antiviral activity of a poly A-poly U complex administered by various routes]. Interferon-inducing and antiviral activity of a synthetic polyribonucleotide, polyaenylic and polyuridylic acid complex (poly A--poly U) was studied comparatively on a model of experimental infection of albino mice caused by Venezuela Horse Encephalomyeliti Viruos (VHEV) using oral and intraperitoneal administration of the complex. It was shown that dm-interferon induction and antiviral effect comparatively high doses of poly A--poly U (Reanal) were required (1 mg/mouse). The highest antiviral effect was observed after a two-fold administration of the drug (24 hours and immediately before infection). It was more evident after intraperitoneal administration of the inductor. The interferon titers in the animal blood serum after intraperitoneal or oral administration of poly A--poly U were almost the same 58=60+/- +/- 18.9 IU50/ml).", "PMID": 952508}
{"id": "pubmed23n0032_31", "title": "[Comparative characteristics of the serotonin and histamine content in the blood of surgical patients before and after antibiotic administration against a background of droperidol, promedol and atropine premedication].", "content": "The effect of intramuscular administration of ceporin and megacillin in single doses of 500 mg and 1000000 Units respectively on serotonin and histamine blood levels in 34 patients against the background of premedication with droperidol, promedol or atropine was studied. The observations showed that after megacillin injection the serotonin blood levels decreased. Ceporin had no effect on serotonin and histamine blood levels.", "contents": "[Comparative characteristics of the serotonin and histamine content in the blood of surgical patients before and after antibiotic administration against a background of droperidol, promedol and atropine premedication]. The effect of intramuscular administration of ceporin and megacillin in single doses of 500 mg and 1000000 Units respectively on serotonin and histamine blood levels in 34 patients against the background of premedication with droperidol, promedol or atropine was studied. The observations showed that after megacillin injection the serotonin blood levels decreased. Ceporin had no effect on serotonin and histamine blood levels.", "PMID": 952509}
{"id": "pubmed23n0032_32", "title": "Sebaceous gland inhibition from combined glucocorticoid-estrogen treatment.", "content": "Sebum production in seven hirsute women was found to be markedly inhibited by low-dosage glucocorticoid therapy, usually 5 to 10 mg of prednisone daily, combined with the cyclic administration of ethinyl estradiol, either 80mug or 100mug daily, in the form of oral contraceptive medication. The average reduction of sebum secretion was 85.7%, from a pretreatment mean value of 3.07 mg of sebum to an average treatment level of 0.44 mg.", "contents": "Sebaceous gland inhibition from combined glucocorticoid-estrogen treatment. Sebum production in seven hirsute women was found to be markedly inhibited by low-dosage glucocorticoid therapy, usually 5 to 10 mg of prednisone daily, combined with the cyclic administration of ethinyl estradiol, either 80mug or 100mug daily, in the form of oral contraceptive medication. The average reduction of sebum secretion was 85.7%, from a pretreatment mean value of 3.07 mg of sebum to an average treatment level of 0.44 mg.", "PMID": 952527}
{"id": "pubmed23n0032_33", "title": "Hyperpigmented tinea versicolor.", "content": "Examination of specimens from lesions of hyperpigmented tinea versicolor and from unaffected skin disclosed that the lesions showed increased stratum corneum turnover (8.2 +/- 0.6 vs 14.8 +/- 0.4 days, respectively), slight to moderate routine histological changes, such as acanthosis and perivascular inflammation, and dramatic electron microscopical changes (larger, singly distributed melanosomes vs small, packaged melanosomes, respectively). Brown hyperpigmentation in tinea versicolor appears to arise from an increase in melanosome size and a change in their epidermal distribution pattern.", "contents": "Hyperpigmented tinea versicolor. Examination of specimens from lesions of hyperpigmented tinea versicolor and from unaffected skin disclosed that the lesions showed increased stratum corneum turnover (8.2 +/- 0.6 vs 14.8 +/- 0.4 days, respectively), slight to moderate routine histological changes, such as acanthosis and perivascular inflammation, and dramatic electron microscopical changes (larger, singly distributed melanosomes vs small, packaged melanosomes, respectively). Brown hyperpigmentation in tinea versicolor appears to arise from an increase in melanosome size and a change in their epidermal distribution pattern.", "PMID": 952528}
{"id": "pubmed23n0032_34", "title": "Intravenous desensitization to mechlorethamine in patients with psoriasis.", "content": "Eight patients with psoriasis who had developed contact allergy to mechlorethamine hydrochloride (nitrogen mustard) were subjected to a regimen of intravenous infusion of small amounts of the drug in an attempt to produce desensitization. Although three of eight developed negative patch tests and were presumed to be desensitized, only one patient was able to use the drug therapeutically, and then only for a period of eight months, after which allergy recurred. The other two patients whose allergic contact dermatitis was abolished by the infusions were unable to use mechlorethamine therapeutically because of pruritus. Seven patients experienced some adverse reaction to the infusion. Intravenous desensitization of psoriatic patients who are allergic to mechlorethamine was not successful enough as a useful clinical procedure to allow them to once again use the drug therapeutically.", "contents": "Intravenous desensitization to mechlorethamine in patients with psoriasis. Eight patients with psoriasis who had developed contact allergy to mechlorethamine hydrochloride (nitrogen mustard) were subjected to a regimen of intravenous infusion of small amounts of the drug in an attempt to produce desensitization. Although three of eight developed negative patch tests and were presumed to be desensitized, only one patient was able to use the drug therapeutically, and then only for a period of eight months, after which allergy recurred. The other two patients whose allergic contact dermatitis was abolished by the infusions were unable to use mechlorethamine therapeutically because of pruritus. Seven patients experienced some adverse reaction to the infusion. Intravenous desensitization of psoriatic patients who are allergic to mechlorethamine was not successful enough as a useful clinical procedure to allow them to once again use the drug therapeutically.", "PMID": 952529}
{"id": "pubmed23n0032_35", "title": "Corticosteroid-induced cutaneous atrophy and telangiectasia. Experimental production associated with weight loss in rats.", "content": "A bioassay for the evaluation of certain adverse effects of various corticosteroids was performed. Twenty-eight daily topical applications of corticosteroids to young rats produced reduction in body-weight gain, atrophy of the skin as determined by double skin-fold thickness micrometer measurement, and mild to severe telangiectasia. This animal model demonstrates corticosteroid-induced skin atrophy and telangiectasia and the correlation of the degree of atrophy and telangiectasia to body-weight change. Nine corticosteroids were evaluated by this method and are listed in terms of increasing severity of side-effects as follows: 1.0% hydrocortisone cream, 0.1% betamethasone valerate cream, 0.025% betamethasone benzoate cream, 0.05% flurandrenolide cream, 0.05% fluocinonide cream, 0.1% dexamethasone cream, and 0.03% flumethasone pivalate cream. Triamcinolone acetonide cream, 0.5%, and 0.2% fluocinolone acetonide cream resulted in death of the animals prior to completion of 28 days of topical application.", "contents": "Corticosteroid-induced cutaneous atrophy and telangiectasia. Experimental production associated with weight loss in rats. A bioassay for the evaluation of certain adverse effects of various corticosteroids was performed. Twenty-eight daily topical applications of corticosteroids to young rats produced reduction in body-weight gain, atrophy of the skin as determined by double skin-fold thickness micrometer measurement, and mild to severe telangiectasia. This animal model demonstrates corticosteroid-induced skin atrophy and telangiectasia and the correlation of the degree of atrophy and telangiectasia to body-weight change. Nine corticosteroids were evaluated by this method and are listed in terms of increasing severity of side-effects as follows: 1.0% hydrocortisone cream, 0.1% betamethasone valerate cream, 0.025% betamethasone benzoate cream, 0.05% flurandrenolide cream, 0.05% fluocinonide cream, 0.1% dexamethasone cream, and 0.03% flumethasone pivalate cream. Triamcinolone acetonide cream, 0.5%, and 0.2% fluocinolone acetonide cream resulted in death of the animals prior to completion of 28 days of topical application.", "PMID": 952530}
{"id": "pubmed23n0032_36", "title": "Allergic and photoallergic dermatitis from diphenhydramine.", "content": "A 52-year-old man developed contact and photoallergic dermatitis caused by diphenhydramine hydrochloride as well as contact dermatitis from paraphenylenediamine. The diphenhydramine photoallergy was elicited by long-wave ultraviolet light. The action spectrum differs from that noted in a recent report in which wavelengths shorter than 320 nm were responsible for eliciting diphenhydramine photoallergy. To the best of my knowledge, this is the third case of diphenhydramine photosensitivity reported in the English literature. The combination of both contact and photosensitivity to diphenhydramine has not previously been described.", "contents": "Allergic and photoallergic dermatitis from diphenhydramine. A 52-year-old man developed contact and photoallergic dermatitis caused by diphenhydramine hydrochloride as well as contact dermatitis from paraphenylenediamine. The diphenhydramine photoallergy was elicited by long-wave ultraviolet light. The action spectrum differs from that noted in a recent report in which wavelengths shorter than 320 nm were responsible for eliciting diphenhydramine photoallergy. To the best of my knowledge, this is the third case of diphenhydramine photosensitivity reported in the English literature. The combination of both contact and photosensitivity to diphenhydramine has not previously been described.", "PMID": 952531}
{"id": "pubmed23n0032_37", "title": "Plaque-type blue nevus.", "content": "Blue nevus may rarely appear in plaque form. It seems to be a benign and asymptomatic lesion, but because of its rarity, no definite prognosis can be given. Only through reporting of additional cases and their follow-up will the prognosis become clear.", "contents": "Plaque-type blue nevus. Blue nevus may rarely appear in plaque form. It seems to be a benign and asymptomatic lesion, but because of its rarity, no definite prognosis can be given. Only through reporting of additional cases and their follow-up will the prognosis become clear.", "PMID": 952532}
{"id": "pubmed23n0032_38", "title": "Arteriovenous fistula. Cutaneous manifestations.", "content": "The association between congenital large-vessel arteriovenous (AV) fistulae of the extremities with Kaposiform acroangiomatosis is well established. Based on pathogenetic considerations, it is likely that many benign, cutaneous angiomatoses represent minute or microscopic AV fistulae. Combined large vessel and small vessel congenital AV fistulae of the extremity would result in the previously mentioned syndrome.", "contents": "Arteriovenous fistula. Cutaneous manifestations. The association between congenital large-vessel arteriovenous (AV) fistulae of the extremities with Kaposiform acroangiomatosis is well established. Based on pathogenetic considerations, it is likely that many benign, cutaneous angiomatoses represent minute or microscopic AV fistulae. Combined large vessel and small vessel congenital AV fistulae of the extremity would result in the previously mentioned syndrome.", "PMID": 952533}
{"id": "pubmed23n0032_39", "title": "Hidradenitis suppurativa following acanthosis nigricans. Report of two cases.", "content": "Two overweight male patients developed acanthosis nigrican of the axillae. Seven to nine months later, the patients developed hidradenitis suppurativa deep within the areas of acanthosis nigricans. On examination of biopsy specimens, the hyperkeratosis of acanthosis nigricans was found to extend into the follicles. In previous reports, Fox-Fordyce disease and pityriasis rubra pilaris have preceded the onset of hidradenitis suppurativa.", "contents": "Hidradenitis suppurativa following acanthosis nigricans. Report of two cases. Two overweight male patients developed acanthosis nigrican of the axillae. Seven to nine months later, the patients developed hidradenitis suppurativa deep within the areas of acanthosis nigricans. On examination of biopsy specimens, the hyperkeratosis of acanthosis nigricans was found to extend into the follicles. In previous reports, Fox-Fordyce disease and pityriasis rubra pilaris have preceded the onset of hidradenitis suppurativa.", "PMID": 952534}
{"id": "pubmed23n0032_40", "title": "Circular indurated lymphangitis of the penis.", "content": "Circular lymphangitis of the penis has been seen in three of our patients, two of whom complained of mild discomfort. A patient with syphilis was initially misdiagnosed because of similar clinical findings. A serologic test for syphilis should be performed in all patients with this disorder.", "contents": "Circular indurated lymphangitis of the penis. Circular lymphangitis of the penis has been seen in three of our patients, two of whom complained of mild discomfort. A patient with syphilis was initially misdiagnosed because of similar clinical findings. A serologic test for syphilis should be performed in all patients with this disorder.", "PMID": 952535}
{"id": "pubmed23n0032_41", "title": "Leukonychia striae.", "content": "A 19-year-old keypuncher had leukonychia striae. The pattern of striae suggested that they occurred as a result of the mechanical stimulation of keypunching.", "contents": "Leukonychia striae. A 19-year-old keypuncher had leukonychia striae. The pattern of striae suggested that they occurred as a result of the mechanical stimulation of keypunching.", "PMID": 952536}
{"id": "pubmed23n0032_42", "title": "Pemphigus foliaceus. Early clinical appearance as dermatitis herpetiformis with eosinophilic spongiosis.", "content": "We encountered an unusual case of pemphigus foliaceus in which there were early clinical signs of dermatitis herpetiformis with eosinophilic spongiosis histologically. Numerous authors have appreciated that eosinophilic spongiosis occurs in early pemphigus foliaceus, usually in the preacantholytic stage when the lesions may clinically simulate dermatitis herpetiformis. Recognition of eosinophilic spongiosis may help differentiate early pemphigus from other vesiculobullous disorders.", "contents": "Pemphigus foliaceus. Early clinical appearance as dermatitis herpetiformis with eosinophilic spongiosis. We encountered an unusual case of pemphigus foliaceus in which there were early clinical signs of dermatitis herpetiformis with eosinophilic spongiosis histologically. Numerous authors have appreciated that eosinophilic spongiosis occurs in early pemphigus foliaceus, usually in the preacantholytic stage when the lesions may clinically simulate dermatitis herpetiformis. Recognition of eosinophilic spongiosis may help differentiate early pemphigus from other vesiculobullous disorders.", "PMID": 952537}
{"id": "pubmed23n0032_43", "title": "Sebaceous glands in the esophagus. First case observed grossly.", "content": "Minute, pale tan macules of the esophageal mucosa observed at autopsy proved, on microscopical study, to be sebaceous glands unaccompanied by hair follicles. Metaplasia of pluripotent cells is postulated as their origin in this purely endodermal tissue, rather than derivation from islands of misplaced epidermis.", "contents": "Sebaceous glands in the esophagus. First case observed grossly. Minute, pale tan macules of the esophageal mucosa observed at autopsy proved, on microscopical study, to be sebaceous glands unaccompanied by hair follicles. Metaplasia of pluripotent cells is postulated as their origin in this purely endodermal tissue, rather than derivation from islands of misplaced epidermis.", "PMID": 952538}
{"id": "pubmed23n0032_44", "title": "Atypical fibroxanthoma masquerading as pyogenic granuloma.", "content": "An atypical fibroxanthoma on the ear of a 82-year-old man appeared clinically as a pyogenic granuloma. Histopathologically, the lesion had been interpreted as a malignant melanoma before its true nature was identified. Familiarity with this cutaneous pseudomalignant growth is essential to prevent radical surgical procedures.", "contents": "Atypical fibroxanthoma masquerading as pyogenic granuloma. An atypical fibroxanthoma on the ear of a 82-year-old man appeared clinically as a pyogenic granuloma. Histopathologically, the lesion had been interpreted as a malignant melanoma before its true nature was identified. Familiarity with this cutaneous pseudomalignant growth is essential to prevent radical surgical procedures.", "PMID": 952539}
{"id": "pubmed23n0032_45", "title": "Larva currens. Cutaneous strongyloidiasis.", "content": "Strongyloides stercoralis causes a distinctive form of cutaneous larva migrans that is recognized as larva currens. The rapid rate of intracutaneous migration, the cutaneous localization, the chronicity of recurrence, and the frequency of urticarial reaction justify its presentation as a clinically distinguishable form of creeping eruption. This clinical complex should be identified as a cutaneous sign of systemic disease.", "contents": "Larva currens. Cutaneous strongyloidiasis. Strongyloides stercoralis causes a distinctive form of cutaneous larva migrans that is recognized as larva currens. The rapid rate of intracutaneous migration, the cutaneous localization, the chronicity of recurrence, and the frequency of urticarial reaction justify its presentation as a clinically distinguishable form of creeping eruption. This clinical complex should be identified as a cutaneous sign of systemic disease.", "PMID": 952540}
{"id": "pubmed23n0032_46", "title": "Clinical longitudinal standards for height, weight, height velocity, weight velocity, and stages of puberty.", "content": "New charts for height, weight, height velocity, and weight velocity are presented for clinical (as opposed to population survey) use. They are based on longitudinal-type growth curves, using the same data as in the British 1965 growth standards. In the velocity standards centiles are given for children who are early- and late-maturing as well as for those who mature at the average age (thus extending the use of the previous charts). Limits of normality for the age of occurrence of the adolescent growth spurt are given and also for the successive stages of penis, testes, and pubic hair development in boys, and for stages of breast and pubic hair development in girls.", "contents": "Clinical longitudinal standards for height, weight, height velocity, weight velocity, and stages of puberty. New charts for height, weight, height velocity, and weight velocity are presented for clinical (as opposed to population survey) use. They are based on longitudinal-type growth curves, using the same data as in the British 1965 growth standards. In the velocity standards centiles are given for children who are early- and late-maturing as well as for those who mature at the average age (thus extending the use of the previous charts). Limits of normality for the age of occurrence of the adolescent growth spurt are given and also for the successive stages of penis, testes, and pubic hair development in boys, and for stages of breast and pubic hair development in girls.", "PMID": 952550}
{"id": "pubmed23n0032_47", "title": "Suspected poisoning in children. Study of the incidence of true poisoning and poisoning scare in a defined population in North East Bristol.", "content": "The distinction between true and suspected poisoning in children has not been made clear in previous work on childhood poisoning. A study of suspected poisoning in children under 15 years of age in a defined population of North East Bristol from November 1970 to July 1973 carried out by the Health Education Council Medical Research Division included 53,000 child-years at risk. The number of suspected poisonings was 3-4/1000 population aged under 15 years per year, with a higher incidence in younger age groups. Detailed investigation of the circumstances of the accidents carried out by a multidisciplinary team showed that at least 65%, and possibly as many as 78% were poisoning scares and not true poisoning. The evidence used by the casualty doctor and by the parents to diagnose poisoning was explored, and in many cases was circumstantial. Children with fathers in nonmanual occupations were over-represented. This may reflect differences in patterns of utilization behaviour rather than true differences in incidence.", "contents": "Suspected poisoning in children. Study of the incidence of true poisoning and poisoning scare in a defined population in North East Bristol. The distinction between true and suspected poisoning in children has not been made clear in previous work on childhood poisoning. A study of suspected poisoning in children under 15 years of age in a defined population of North East Bristol from November 1970 to July 1973 carried out by the Health Education Council Medical Research Division included 53,000 child-years at risk. The number of suspected poisonings was 3-4/1000 population aged under 15 years per year, with a higher incidence in younger age groups. Detailed investigation of the circumstances of the accidents carried out by a multidisciplinary team showed that at least 65%, and possibly as many as 78% were poisoning scares and not true poisoning. The evidence used by the casualty doctor and by the parents to diagnose poisoning was explored, and in many cases was circumstantial. Children with fathers in nonmanual occupations were over-represented. This may reflect differences in patterns of utilization behaviour rather than true differences in incidence.", "PMID": 952551}
{"id": "pubmed23n0032_48", "title": "Pet sensitivities in asthmatic children.", "content": "A history of pet contact and/or apparent clinical sensitivity was obtained in 65 (55%) of 118 unselected asthmatic children. These 65 children were skin tested and their sera examined for specific IgE using the radioallergosorbent test. Those children who had apparent clinical sensitivities had larger skin test reactions and were more likely to have positive specific IgE results than those without apparent sensitivities. Positive skin tests were very common (80%), but the larger the skin test reaction (weal diameter greater than 4 mm diameter) the more likely was there to be a positive history or a positive specific IgE result. Hence a large skin test reaction can provide a helpful pointer to animal allergy of clinical importance. Commercially available animal extracts have limitations for diagnostic tests. A questionnaire survey of 150 day schools emphasized the potential opportunities for contact with animal allergens at school.", "contents": "Pet sensitivities in asthmatic children. A history of pet contact and/or apparent clinical sensitivity was obtained in 65 (55%) of 118 unselected asthmatic children. These 65 children were skin tested and their sera examined for specific IgE using the radioallergosorbent test. Those children who had apparent clinical sensitivities had larger skin test reactions and were more likely to have positive specific IgE results than those without apparent sensitivities. Positive skin tests were very common (80%), but the larger the skin test reaction (weal diameter greater than 4 mm diameter) the more likely was there to be a positive history or a positive specific IgE result. Hence a large skin test reaction can provide a helpful pointer to animal allergy of clinical importance. Commercially available animal extracts have limitations for diagnostic tests. A questionnaire survey of 150 day schools emphasized the potential opportunities for contact with animal allergens at school.", "PMID": 952552}
{"id": "pubmed23n0032_49", "title": "Cyproterone acetate in treatment of precocious puberty.", "content": "Twenty-nine children (23 girls, 6 boys) with precocious puberty were treated with cyproterone acetate for various periods of time ranging from 6 months to 3 years 4 months. They received an oral dose ranging from 70-150 mg/m2 per day, or an intramuscular depot injection once a fortnight or once a month at a dose ranging from 107-230 mg/m2. Both forms of therapy were found to suppress the signs of sexual maturation, but the oral form proved to be superior. Only the younger patients with a bone age under 11 years showed a beneficial effect upon linear growth and bone maturation. No side effects were noted, but additional advantageous effects upon behaviour and sociability were. It is concluded that at present cyproterone acetate by mouth is the drug of choice in the treatment of precocious puberty. The treatment should be initiated as early as possible to attain maximum benefit.", "contents": "Cyproterone acetate in treatment of precocious puberty. Twenty-nine children (23 girls, 6 boys) with precocious puberty were treated with cyproterone acetate for various periods of time ranging from 6 months to 3 years 4 months. They received an oral dose ranging from 70-150 mg/m2 per day, or an intramuscular depot injection once a fortnight or once a month at a dose ranging from 107-230 mg/m2. Both forms of therapy were found to suppress the signs of sexual maturation, but the oral form proved to be superior. Only the younger patients with a bone age under 11 years showed a beneficial effect upon linear growth and bone maturation. No side effects were noted, but additional advantageous effects upon behaviour and sociability were. It is concluded that at present cyproterone acetate by mouth is the drug of choice in the treatment of precocious puberty. The treatment should be initiated as early as possible to attain maximum benefit.", "PMID": 952553}
{"id": "pubmed23n0032_50", "title": "Rifamycin in neonatal flavobacteria meningitis.", "content": "Three newborn infants with meningitis due to Flavobacterium meningosepticum were treated with rifamycin administered parenterally and directly into the cerebral ventricles. Antibiotic concentrations of blood and cerebrospinal fluid (CSF) were monitored during treatment. There was rapid sterilization of the CSF after this antibiotic. Jaundice was the only toxicity noted. All 3 infants developed hydrocephalus and are shunt dependent. Two of them are otherwise free of neurological complications and are developing normally. Rifamycin is a safe and effective antibiotic in this form of neonatal meningitis.", "contents": "Rifamycin in neonatal flavobacteria meningitis. Three newborn infants with meningitis due to Flavobacterium meningosepticum were treated with rifamycin administered parenterally and directly into the cerebral ventricles. Antibiotic concentrations of blood and cerebrospinal fluid (CSF) were monitored during treatment. There was rapid sterilization of the CSF after this antibiotic. Jaundice was the only toxicity noted. All 3 infants developed hydrocephalus and are shunt dependent. Two of them are otherwise free of neurological complications and are developing normally. Rifamycin is a safe and effective antibiotic in this form of neonatal meningitis.", "PMID": 952554}
{"id": "pubmed23n0032_51", "title": "Reversible intestinal mucosal abnormality in acrodermatitis enteropathica.", "content": "In 3 cases of acrodermatitis enteropathica duodenal biopsy performed at the outset of treatment showed a similar abnormality of the intestinal mucosa. Further biopsies taken during treatment showed progressive improvement of the intestinal mucosa with subsequent complete restoration of the normal cellular and villous pattern. The initial treatment was with expressed human breast milk and oral di-iodohydroxyquinoline. The latter was continued alone and later replaced by zinc sulphate. Changes in the intestinal epithelial cells and inflammatory cell infiltration of the lamina propria still detectable on di-iodohydroxyquinoline therapy reverted to normal with oral zinc.", "contents": "Reversible intestinal mucosal abnormality in acrodermatitis enteropathica. In 3 cases of acrodermatitis enteropathica duodenal biopsy performed at the outset of treatment showed a similar abnormality of the intestinal mucosa. Further biopsies taken during treatment showed progressive improvement of the intestinal mucosa with subsequent complete restoration of the normal cellular and villous pattern. The initial treatment was with expressed human breast milk and oral di-iodohydroxyquinoline. The latter was continued alone and later replaced by zinc sulphate. Changes in the intestinal epithelial cells and inflammatory cell infiltration of the lamina propria still detectable on di-iodohydroxyquinoline therapy reverted to normal with oral zinc.", "PMID": 952555}
{"id": "pubmed23n0032_52", "title": "Medullary carcinoma of thyroid gland in a girl aged 10 years.", "content": "A healthy girl of 10 years presented with lumps on her tongue, shown on biopsy to be neuromas. She had had operations to her feet for pes cavus and was relatively long-limbed but had no other 'Marfanoid' features. She had a high plasma calcitonin level. At operation a normal sized thyroid gland was totally removed. It contained two discrete masses of malignant C-cells and diffuse foci elsewhere. The plasma calcitonin fell slowly to normal on follow-up but was noted to be rising 2 1/2 years later. We stress the importance of making this diagnosis as early as possible and mention briefly another child aged 15 months, similarly diagnosed and operated upon.", "contents": "Medullary carcinoma of thyroid gland in a girl aged 10 years. A healthy girl of 10 years presented with lumps on her tongue, shown on biopsy to be neuromas. She had had operations to her feet for pes cavus and was relatively long-limbed but had no other 'Marfanoid' features. She had a high plasma calcitonin level. At operation a normal sized thyroid gland was totally removed. It contained two discrete masses of malignant C-cells and diffuse foci elsewhere. The plasma calcitonin fell slowly to normal on follow-up but was noted to be rising 2 1/2 years later. We stress the importance of making this diagnosis as early as possible and mention briefly another child aged 15 months, similarly diagnosed and operated upon.", "PMID": 952556}
{"id": "pubmed23n0032_53", "title": "Peritoneal dialysis and exchange transfusion in a neonate with argininosuccinic aciduria.", "content": "Peritoneal dialysis rapidly reduced blood ammonia concentration in this child with arginino-succinic acid-lyase deficiency, whereas exchange transfusion did not. Yet this reduction in plasma ammonia level did not produce clinical improvement. We speculate that the effects of ammonia intoxication on the highly susceptible neonatal metabolism are due to an accumulation of toxic products and to an altered energy metabolism. Both aspects must be considered in any attempt to treat congenital hyperammonaemia.", "contents": "Peritoneal dialysis and exchange transfusion in a neonate with argininosuccinic aciduria. Peritoneal dialysis rapidly reduced blood ammonia concentration in this child with arginino-succinic acid-lyase deficiency, whereas exchange transfusion did not. Yet this reduction in plasma ammonia level did not produce clinical improvement. We speculate that the effects of ammonia intoxication on the highly susceptible neonatal metabolism are due to an accumulation of toxic products and to an altered energy metabolism. Both aspects must be considered in any attempt to treat congenital hyperammonaemia.", "PMID": 952557}
{"id": "pubmed23n0032_54", "title": "Decreased antihaemophilic globulin and leucocyte response to epinephrine in preterm infants.", "content": "Twenty-one preterm and 23 term neonates, 13 splenectomized children and one with congenital asplenia, and 20 normal children were examined for plasma antihaemophilic activity and for blood leucocyte levels before and 30 minutes after a subcutaneous injection of epinephrine 0-01 mg/kg. The basal values for antihaemophilic activity were similar for the 4 groups. The response to epinephrine was a trivial rise in antihaemophilic activity in the preterm group, while the rise in the term newborns was comparable to that of the normal children. The asplenic children all showed a trivial rise. The leucocyte response was also negligible in both the preterm neonates and asplenic groups, while in the term infants it was comparable to that seen in the normal children. These results may indicate an incapacity of the preterm newborn infant's reticuloendothelial system and spleen to react to other challenges, such as bacterial infection.", "contents": "Decreased antihaemophilic globulin and leucocyte response to epinephrine in preterm infants. Twenty-one preterm and 23 term neonates, 13 splenectomized children and one with congenital asplenia, and 20 normal children were examined for plasma antihaemophilic activity and for blood leucocyte levels before and 30 minutes after a subcutaneous injection of epinephrine 0-01 mg/kg. The basal values for antihaemophilic activity were similar for the 4 groups. The response to epinephrine was a trivial rise in antihaemophilic activity in the preterm group, while the rise in the term newborns was comparable to that of the normal children. The asplenic children all showed a trivial rise. The leucocyte response was also negligible in both the preterm neonates and asplenic groups, while in the term infants it was comparable to that seen in the normal children. These results may indicate an incapacity of the preterm newborn infant's reticuloendothelial system and spleen to react to other challenges, such as bacterial infection.", "PMID": 952558}
{"id": "pubmed23n0032_55", "title": "Manpower goals in American surgery. Implications for residency training. Future surgical manpower in the framework of total United States physicians.", "content": "Constraints on manpower are intrinsic in the establishment of standards of excellence. When such constraints are exerted by individual Boards, Societies, Colleges or Academies they should act to improve the quality of care; their weakness lies in their lack of control over non-members, or those who have failed to pass the examinations. Such manpower constraints become specific objectives or goals when the number of accredited specialists is specifically related to the size of the population served. Any such manpower planning must recognize the many uncertainties in the future of American medicine, and maintain wide elasticity in the planning process. Social and economic pressures render the consideration of specific manpower goals essential at this time. Data from the national surgical study (SOSSUS) make it possible to consider such goals. Manpower objectives for surgery or any other branch of medicine should be considered as a part of the total medical manpower outlook for the United States. Pressures to reduce the number of surgeons entering practice are notable at this time. These should be evaluated against other pressures to maintain or increase the number of hospital-based specialists in all fields as the total number of practitioners undergoes a major expansion over the next 25 years, and the pressure for specialty care is thereby increased. A reasonable balance between these two pressures would be a manpower goal for surgery that allowed a modest growth rate over the next 25-50 years. An example of such is the goal of limiting surgical practitioner growth to a 1% increase in the ratio to population, every 5 years. This would be in sharp contrast to the continuous explosive growth of numbers of surgeons, since World War II.", "contents": "Manpower goals in American surgery. Implications for residency training. Future surgical manpower in the framework of total United States physicians. Constraints on manpower are intrinsic in the establishment of standards of excellence. When such constraints are exerted by individual Boards, Societies, Colleges or Academies they should act to improve the quality of care; their weakness lies in their lack of control over non-members, or those who have failed to pass the examinations. Such manpower constraints become specific objectives or goals when the number of accredited specialists is specifically related to the size of the population served. Any such manpower planning must recognize the many uncertainties in the future of American medicine, and maintain wide elasticity in the planning process. Social and economic pressures render the consideration of specific manpower goals essential at this time. Data from the national surgical study (SOSSUS) make it possible to consider such goals. Manpower objectives for surgery or any other branch of medicine should be considered as a part of the total medical manpower outlook for the United States. Pressures to reduce the number of surgeons entering practice are notable at this time. These should be evaluated against other pressures to maintain or increase the number of hospital-based specialists in all fields as the total number of practitioners undergoes a major expansion over the next 25 years, and the pressure for specialty care is thereby increased. A reasonable balance between these two pressures would be a manpower goal for surgery that allowed a modest growth rate over the next 25-50 years. An example of such is the goal of limiting surgical practitioner growth to a 1% increase in the ratio to population, every 5 years. This would be in sharp contrast to the continuous explosive growth of numbers of surgeons, since World War II.", "PMID": 952562}
{"id": "pubmed23n0032_56", "title": "Acute cholecystitis as a postoperative complication.", "content": "The clinical course and management of 40 patients who underwent operation for acute cholecystitis developing as a postoperative complication were reviewed. Of note was the mortality of 47%, the high incidence of gangrene, perforation, empyema, and cholangitis, and the atypical clinical presentation of acute cholecystitis under these conditions. Awareness of this possible complication, knowledge of its clinical features, and early surgical intervention are important facets of successful management.", "contents": "Acute cholecystitis as a postoperative complication. The clinical course and management of 40 patients who underwent operation for acute cholecystitis developing as a postoperative complication were reviewed. Of note was the mortality of 47%, the high incidence of gangrene, perforation, empyema, and cholangitis, and the atypical clinical presentation of acute cholecystitis under these conditions. Awareness of this possible complication, knowledge of its clinical features, and early surgical intervention are important facets of successful management.", "PMID": 952563}
{"id": "pubmed23n0032_57", "title": "Leiomyosarcoma of the inferior vena cava: report of a case and review of the literature.", "content": "Leiomyosarcoma of the inferior vena cava is a rare and usually fatal disease. Only 45 cases have been reported in the world literature. The authors add one case and review all previous reports. The disease is one primarily of elderly women, arising from the middle third of the inferior vena cava with a wide range of symptomatology, varying form the Budd-Chiari syndrome to a few asymptomatic cases. The tumor may grow very slowly or occasionally very rapidly, shows extensive local invasion, and metastasizes more frequently than previously believed. While radiotherapy and chemotherapy have not been adequately studied, aggresive surgical intervention using the recent advances in vascular surgery appears to offer long term palliation and hopefully cures of this disease in the future.", "contents": "Leiomyosarcoma of the inferior vena cava: report of a case and review of the literature. Leiomyosarcoma of the inferior vena cava is a rare and usually fatal disease. Only 45 cases have been reported in the world literature. The authors add one case and review all previous reports. The disease is one primarily of elderly women, arising from the middle third of the inferior vena cava with a wide range of symptomatology, varying form the Budd-Chiari syndrome to a few asymptomatic cases. The tumor may grow very slowly or occasionally very rapidly, shows extensive local invasion, and metastasizes more frequently than previously believed. While radiotherapy and chemotherapy have not been adequately studied, aggresive surgical intervention using the recent advances in vascular surgery appears to offer long term palliation and hopefully cures of this disease in the future.", "PMID": 952564}
{"id": "pubmed23n0032_58", "title": "Anastomotic aneurysms following arterial grafting: A 10-year experience.", "content": "A multi-faceted pathogenesis of vascular anastomotic aneurysms encompassing both the earlier and modern eras of vascular surgery has been proposed. A review of the clinical records of patients with such aneurysms suggests that while silk sutures were an important causative factor in the earlier era, this complication continues to occur with the more recent use of synthetic suture material. Future examination of the data suggests that the location of the anastomosis remains important in the development of these aneurysms. Moreover, operative findings suggest that graft tension, shearing and vibration forces along the suture line and degeneration of the host vessel are the more important causative factors today.", "contents": "Anastomotic aneurysms following arterial grafting: A 10-year experience. A multi-faceted pathogenesis of vascular anastomotic aneurysms encompassing both the earlier and modern eras of vascular surgery has been proposed. A review of the clinical records of patients with such aneurysms suggests that while silk sutures were an important causative factor in the earlier era, this complication continues to occur with the more recent use of synthetic suture material. Future examination of the data suggests that the location of the anastomosis remains important in the development of these aneurysms. Moreover, operative findings suggest that graft tension, shearing and vibration forces along the suture line and degeneration of the host vessel are the more important causative factors today.", "PMID": 952565}
{"id": "pubmed23n0032_59", "title": "Physical properties of surgical suture materials: Stress-strain relationship, stress-relaxation and irreversible elongation.", "content": "The stress-strain relationship, the stress-relaxation and the irreversible elongation of different suture materials were investigated. It was found that the distensibility of the fibers varied greatly. Most fibers were found to be chiefly elastic but some fibers were elastic when a moderate load was applied and plastic when the load increased. Some fibers, especially Prolene, were plastic also when moderate load was applied. The importance of information concerning these and other physical properties of surgical suture material is pointed out. In the future, surgeons will not have to accept suture materials with unknown physical properties but will demand fibers with defined properties for every purpose.", "contents": "Physical properties of surgical suture materials: Stress-strain relationship, stress-relaxation and irreversible elongation. The stress-strain relationship, the stress-relaxation and the irreversible elongation of different suture materials were investigated. It was found that the distensibility of the fibers varied greatly. Most fibers were found to be chiefly elastic but some fibers were elastic when a moderate load was applied and plastic when the load increased. Some fibers, especially Prolene, were plastic also when moderate load was applied. The importance of information concerning these and other physical properties of surgical suture material is pointed out. In the future, surgeons will not have to accept suture materials with unknown physical properties but will demand fibers with defined properties for every purpose.", "PMID": 952566}
{"id": "pubmed23n0032_60", "title": "The effect of vagotomy antrectomy or vagotomy pyloroplasty on the response of the antrum to meat extract in duodenal ulcer patients.", "content": "In 9 normal and 42 duodenal ulcer patients, acid and gastrin studies were performed. Basal, Oxo and Histalog stimulated acid secretion was conducted on each patient. In 24 patients post vagotomy pyloroplasty or vagotomy antrectomy, these studies were repeated within three months after surgery. Two groups of duodenal ulcer patients were identified; those who did respond and those who did not respond to Oxo stimulation. In the \"responders,\" Oxo stimulated acid output and gastrin secretion increased significantly over basal values. Both vagotomy pyloroplasty and vagotomy antrectomy caused a similar significant decrease in Oxo and Histalog stimulated acid output. In two patients with incomplete vagotomy, antrectomy, but not pyloroplasty abolished the Oxo stimulated acid response. These data suggest that OXO stimulation test can select patients with a significant antral component in whom vagotomy and antrectomy would be the appropriate procedure. Our results also indicate that antrectomy will protect against recurrent ulceration in patients with incomplete vagotomy and may explain the lower incidence of stomal ulceration in patients with vagotomy antrectomy, compared to vagotomy pyloroplasty.", "contents": "The effect of vagotomy antrectomy or vagotomy pyloroplasty on the response of the antrum to meat extract in duodenal ulcer patients. In 9 normal and 42 duodenal ulcer patients, acid and gastrin studies were performed. Basal, Oxo and Histalog stimulated acid secretion was conducted on each patient. In 24 patients post vagotomy pyloroplasty or vagotomy antrectomy, these studies were repeated within three months after surgery. Two groups of duodenal ulcer patients were identified; those who did respond and those who did not respond to Oxo stimulation. In the \"responders,\" Oxo stimulated acid output and gastrin secretion increased significantly over basal values. Both vagotomy pyloroplasty and vagotomy antrectomy caused a similar significant decrease in Oxo and Histalog stimulated acid output. In two patients with incomplete vagotomy, antrectomy, but not pyloroplasty abolished the Oxo stimulated acid response. These data suggest that OXO stimulation test can select patients with a significant antral component in whom vagotomy and antrectomy would be the appropriate procedure. Our results also indicate that antrectomy will protect against recurrent ulceration in patients with incomplete vagotomy and may explain the lower incidence of stomal ulceration in patients with vagotomy antrectomy, compared to vagotomy pyloroplasty.", "PMID": 952567}
{"id": "pubmed23n0032_61", "title": "Comparison of ancrod and heparin as anticoagulants following endarterectomy in the dog.", "content": "An experimental model of surgically-induced arterial thrombosis was devised using the femoral arteries of dogs. Within 7 days, 67% of the arteries became completely thrombosed and only 12% remained compeletly patent. In the group of dogs that received low-dose heparin, 69% of the vessels were completely thrombosed and 6% remained completely patent. In the group of dogs treated with low-dose Ancrod to induce partial defibrination, 75% remained completely patent while only 19% of their femoral arteries were completely thrombosed. Although the ancrod was effective in preventing arterial thrombosis, 88% of the wounds showed moderate to severe separations. Most likely the absence of a fibrin lattice, necessary for the securement and growth of fibroblasts as the wound heals, explains this latter effect. Thus while Ancrod may become useful as an anticoagulant in certain clinical situations, it should not be used in proximity to surgery. Finally, in these studies of acute arterial thromboses, low-dose heparin therapy offered no protective effect.", "contents": "Comparison of ancrod and heparin as anticoagulants following endarterectomy in the dog. An experimental model of surgically-induced arterial thrombosis was devised using the femoral arteries of dogs. Within 7 days, 67% of the arteries became completely thrombosed and only 12% remained compeletly patent. In the group of dogs that received low-dose heparin, 69% of the vessels were completely thrombosed and 6% remained completely patent. In the group of dogs treated with low-dose Ancrod to induce partial defibrination, 75% remained completely patent while only 19% of their femoral arteries were completely thrombosed. Although the ancrod was effective in preventing arterial thrombosis, 88% of the wounds showed moderate to severe separations. Most likely the absence of a fibrin lattice, necessary for the securement and growth of fibroblasts as the wound heals, explains this latter effect. Thus while Ancrod may become useful as an anticoagulant in certain clinical situations, it should not be used in proximity to surgery. Finally, in these studies of acute arterial thromboses, low-dose heparin therapy offered no protective effect.", "PMID": 952568}
{"id": "pubmed23n0032_62", "title": "Reduction of early occlusions in the high risk peripheral saphenous vein graft.", "content": "Early thrombosis of saphenous vein grafts can only reduce long-term patency. There exists a high risk category of patients who can be identified preoperatively where early failure is increased above the 10-20% which is attributed to technical errors. A venous side-arm indwelling catheter offers a safe means of reducing early thrombosis, promptly diagnosing the occlusion when it does occur, and more clearly delineating a course of corrective action. The venous side-arm catheter provides a means by which the vascular bed at highest risk may be regionally anticoagulated while allowing systemic clotting times to remain near normal. In addition, vasoactive drugs may be infused directly into the arterial tree and perhaps most importantly, rapid and atraumatic arteriograms can be obtained postoperatively at the bedside for evaluation of questionable perfusion. Regional heparinization and postoperative arterigraphy by means to the side-branch catheter technique was used in twelve patients who otherwise had an expected occlusion rate of 75% or more with an actual early occlusion incidence of zero.", "contents": "Reduction of early occlusions in the high risk peripheral saphenous vein graft. Early thrombosis of saphenous vein grafts can only reduce long-term patency. There exists a high risk category of patients who can be identified preoperatively where early failure is increased above the 10-20% which is attributed to technical errors. A venous side-arm indwelling catheter offers a safe means of reducing early thrombosis, promptly diagnosing the occlusion when it does occur, and more clearly delineating a course of corrective action. The venous side-arm catheter provides a means by which the vascular bed at highest risk may be regionally anticoagulated while allowing systemic clotting times to remain near normal. In addition, vasoactive drugs may be infused directly into the arterial tree and perhaps most importantly, rapid and atraumatic arteriograms can be obtained postoperatively at the bedside for evaluation of questionable perfusion. Regional heparinization and postoperative arterigraphy by means to the side-branch catheter technique was used in twelve patients who otherwise had an expected occlusion rate of 75% or more with an actual early occlusion incidence of zero.", "PMID": 952569}
{"id": "pubmed23n0032_63", "title": "Gastrocolic fistulae in benign peptic ulcer disease.", "content": "Occurence of gastroenteric fistulae in non-surgically treated peptic ulcer disease is rare as suggested by only 26 cases published in the literature so far. We had the opportunity to care for 3 patients with this problem in a period of one year. At our institution, a search of the medical records dating back to 1955 yielded one additional case. Most of our patients had a history of salicylate or corticosteroid intake. We present here the details of these cases and discuss the possible role of the location of the ulcer and ulcerogenic anti-inflammatory drug ingestion in the causation of gastroenteric fistula. We believe the incidence of this uncommon entity may rise significantly with the more common use of salicylates and corticosteroids.", "contents": "Gastrocolic fistulae in benign peptic ulcer disease. Occurence of gastroenteric fistulae in non-surgically treated peptic ulcer disease is rare as suggested by only 26 cases published in the literature so far. We had the opportunity to care for 3 patients with this problem in a period of one year. At our institution, a search of the medical records dating back to 1955 yielded one additional case. Most of our patients had a history of salicylate or corticosteroid intake. We present here the details of these cases and discuss the possible role of the location of the ulcer and ulcerogenic anti-inflammatory drug ingestion in the causation of gastroenteric fistula. We believe the incidence of this uncommon entity may rise significantly with the more common use of salicylates and corticosteroids.", "PMID": 952570}
{"id": "pubmed23n0032_64", "title": "Pyogenic liver abscess secondary to asymptomatic sigmoid diverticulitis.", "content": "A patient with multiple pyogenic abscesses in both lobes of the liver secondary to asymptomatic sigmoid diverticulitis is presented. The rarity of this illness is noted. It is suggested that barium enema be performed in patients who present with pyogenic liver abscess of unknown etiology because of the association with asymptomatic sigmoid diverticulitis.", "contents": "Pyogenic liver abscess secondary to asymptomatic sigmoid diverticulitis. A patient with multiple pyogenic abscesses in both lobes of the liver secondary to asymptomatic sigmoid diverticulitis is presented. The rarity of this illness is noted. It is suggested that barium enema be performed in patients who present with pyogenic liver abscess of unknown etiology because of the association with asymptomatic sigmoid diverticulitis.", "PMID": 952571}
{"id": "pubmed23n0032_65", "title": "Disposition kinetics of ketamine in the domestic cat.", "content": "The disposition kinetics and systemic availability of ketamine, a dissociative anaesthetic agent, was studied in normal domestic cats. A similar dose (25 mg/kg) of ketamine hydrochloride was administered by the i.v. and i.m. routes; drug concentrations in plasma were measured by a gas-liquid chromatographic procedure. A rapid distribution phase (t1/2 (alpha) = 3 min) was followed by a slower first-order elimination phase. The half-life of the drug (66.9 +/- 24.1 min) was independent of the route of parenteral administration. Absorption from i.m. site of administration was rapid, with peak plasma level at 10 min, and ca. 92 per cent of the dose was available systemically. Extent of plasma protein binding, measured in vitro at 5 and 20 mug/ml by equilibrium dialysis technique, was 53 per cent and independent of ketamine concentration. Simulated plasma and tissue level curves, which were generated by an analogue computer programmed with the individual rate constants of the two-compartment open model, showed that 10 and 15 per cent of the dose were present in the central and peripheral compartments, respectively, at 90 min after i.v. administration of the drug. Peak tissue level of 42 per cent of the dose was reached at 12 to 15 min. Parenteral administration of ketamine, at the dosage rate studied, quickly produced an immobilizing effect of variable duration (0.75 to 1.75 hr) in normal cats.", "contents": "Disposition kinetics of ketamine in the domestic cat. The disposition kinetics and systemic availability of ketamine, a dissociative anaesthetic agent, was studied in normal domestic cats. A similar dose (25 mg/kg) of ketamine hydrochloride was administered by the i.v. and i.m. routes; drug concentrations in plasma were measured by a gas-liquid chromatographic procedure. A rapid distribution phase (t1/2 (alpha) = 3 min) was followed by a slower first-order elimination phase. The half-life of the drug (66.9 +/- 24.1 min) was independent of the route of parenteral administration. Absorption from i.m. site of administration was rapid, with peak plasma level at 10 min, and ca. 92 per cent of the dose was available systemically. Extent of plasma protein binding, measured in vitro at 5 and 20 mug/ml by equilibrium dialysis technique, was 53 per cent and independent of ketamine concentration. Simulated plasma and tissue level curves, which were generated by an analogue computer programmed with the individual rate constants of the two-compartment open model, showed that 10 and 15 per cent of the dose were present in the central and peripheral compartments, respectively, at 90 min after i.v. administration of the drug. Peak tissue level of 42 per cent of the dose was reached at 12 to 15 min. Parenteral administration of ketamine, at the dosage rate studied, quickly produced an immobilizing effect of variable duration (0.75 to 1.75 hr) in normal cats.", "PMID": 952572}
{"id": "pubmed23n0032_66", "title": "Carbamazepine pharmacokinetics in young, adult and pregnant rats. Relation to pharmacological effects.", "content": "Adult, male, female and pregnant rats were treated with single and repeated doses of carbamazepine (CBZ). The time course of the drug concentrations in plasma and tissues was followed. In all cases, data on plasma levels were subjected to pharmacokinetic analyses. Attempts were made to relate pharmacokinetic properties of carbamazepine to its effect on pentobarbital sleeping time and on protection against electroshock, after acute and repeated administration: --it was found that male rats eliminate carbamazepine faster than females: the total body clearance (TBC) was 16 ml/min/kg and 9.4 ml/min/kg, respectively. Two dose levels (25 and 50 mg/kg) had the same pharmacokinetic properties in young rats. Pregnant rats clear CBZ to a lesser extent than controls. --CBZ was found to accelerate its own elimination after repeated administration in both adult and young rats as revealed by the shortening of its half-life and an increase of 50% in clearance. Moreover, the protection against electroshock was significantly reduced after repeated administration, compared with a single-dose administration. Repeated administration of CBZ in rats shortens pentobarbital sleeping time and decreases the pentobarbital brain level significantly.", "contents": "Carbamazepine pharmacokinetics in young, adult and pregnant rats. Relation to pharmacological effects. Adult, male, female and pregnant rats were treated with single and repeated doses of carbamazepine (CBZ). The time course of the drug concentrations in plasma and tissues was followed. In all cases, data on plasma levels were subjected to pharmacokinetic analyses. Attempts were made to relate pharmacokinetic properties of carbamazepine to its effect on pentobarbital sleeping time and on protection against electroshock, after acute and repeated administration: --it was found that male rats eliminate carbamazepine faster than females: the total body clearance (TBC) was 16 ml/min/kg and 9.4 ml/min/kg, respectively. Two dose levels (25 and 50 mg/kg) had the same pharmacokinetic properties in young rats. Pregnant rats clear CBZ to a lesser extent than controls. --CBZ was found to accelerate its own elimination after repeated administration in both adult and young rats as revealed by the shortening of its half-life and an increase of 50% in clearance. Moreover, the protection against electroshock was significantly reduced after repeated administration, compared with a single-dose administration. Repeated administration of CBZ in rats shortens pentobarbital sleeping time and decreases the pentobarbital brain level significantly.", "PMID": 952573}
{"id": "pubmed23n0032_67", "title": "Hexachlorophene metabolism in rats: the hepatic route.", "content": "The terminal elimination rate of radioactivity from the bile of bile-duct cannulated rats, that had received hexachlorophene-14C via the hepatic portal vein, had an apparent first-order half-life of about 10 hr. Tissue distribution studies in these rats indicated that 35-47% of the carbon-14 of the dose was eliminated through the bile within 24 hr. Significant amounts of radioactivity were also found in the liver and carcass at that time; the brain was the only tissue that did not consistently have much higher concentrations of carbon-14 than the blood. The rate limiting step in the disposition of hexachlorophene may be excretion into the bile. A bile-feeding technique demonstrated enterohepatic circulation.", "contents": "Hexachlorophene metabolism in rats: the hepatic route. The terminal elimination rate of radioactivity from the bile of bile-duct cannulated rats, that had received hexachlorophene-14C via the hepatic portal vein, had an apparent first-order half-life of about 10 hr. Tissue distribution studies in these rats indicated that 35-47% of the carbon-14 of the dose was eliminated through the bile within 24 hr. Significant amounts of radioactivity were also found in the liver and carcass at that time; the brain was the only tissue that did not consistently have much higher concentrations of carbon-14 than the blood. The rate limiting step in the disposition of hexachlorophene may be excretion into the bile. A bile-feeding technique demonstrated enterohepatic circulation.", "PMID": 952574}
{"id": "pubmed23n0032_68", "title": "[Interaction of tricyclic antidepressants with noradrenaline and 5-hydroxytryptamine in peripheral preparations of the rat].", "content": "The potentiation of the noradrenaline (NA) actions by antidepressant drugs was studied in vitro on the rat vas deferens and in vivo on the rat blood pressure. A new dibenzazepine compound, LM 208, and two antiparkinsonian drugs, orphenadrine and its demethylated derivative, were compared to well known antidepressants. The modification by all these agents of the rat uterus response to 5-hydroxytryptamine (5-HT) was also investigated. The possible use of these interactions as models for NA and 5-HT uptake inhibition is discussed.", "contents": "[Interaction of tricyclic antidepressants with noradrenaline and 5-hydroxytryptamine in peripheral preparations of the rat]. The potentiation of the noradrenaline (NA) actions by antidepressant drugs was studied in vitro on the rat vas deferens and in vivo on the rat blood pressure. A new dibenzazepine compound, LM 208, and two antiparkinsonian drugs, orphenadrine and its demethylated derivative, were compared to well known antidepressants. The modification by all these agents of the rat uterus response to 5-hydroxytryptamine (5-HT) was also investigated. The possible use of these interactions as models for NA and 5-HT uptake inhibition is discussed.", "PMID": 952575}
{"id": "pubmed23n0032_69", "title": "The influence of meprobamate on heart rate in the conscious dog.", "content": "The effects of meprobamate on heart rate in the unanesthetized dog were compared under two experimental conditions: (1) In the animal equipped with a telemetry device, in which the heart rate was on average 70/min over a 24 hour period, i.v. injection of 20 mg/kg of meprobamate tended to increase heart rate, a dose of 50 mg/kg provoking a significant tachycardia; (2) In the untrained dog restrained on a table, in which the heart rate ranged from 100 to 110/min, i.v. doses of 20 and 30 mg/kg of meprobamate did not lower heart rate, notwithstanding a significant sedative effect whereas a dose of 50 mg/kg provoked tachycardia; atropine-induced cardio-acceleration was not influenced by these doses of meprobamate.", "contents": "The influence of meprobamate on heart rate in the conscious dog. The effects of meprobamate on heart rate in the unanesthetized dog were compared under two experimental conditions: (1) In the animal equipped with a telemetry device, in which the heart rate was on average 70/min over a 24 hour period, i.v. injection of 20 mg/kg of meprobamate tended to increase heart rate, a dose of 50 mg/kg provoking a significant tachycardia; (2) In the untrained dog restrained on a table, in which the heart rate ranged from 100 to 110/min, i.v. doses of 20 and 30 mg/kg of meprobamate did not lower heart rate, notwithstanding a significant sedative effect whereas a dose of 50 mg/kg provoked tachycardia; atropine-induced cardio-acceleration was not influenced by these doses of meprobamate.", "PMID": 952576}
{"id": "pubmed23n0032_70", "title": "Release and receptor stimulating properties of p-tyramine in rat brain.", "content": "Radiolabeled p-tyramine could be released from rat striatal slices, which were previously allowed to accumulate this amine, upon depolarization with media containing 40 mM potassium. This depolarization-induced release did not occur in the absence of calcium ions in the medium and over 75% of the labeled material released was identified chromatographically as tyramine. The possible existence of a direct receptor-stimulating activity of p-tyramine was examined by injecting this amine intraventricularly into rats, after pretreatment with various drugs, which inhibit catecholaminergic function, and measuring their motor activity. If injected into rats, which were pretreated with reserpine or alpha-methyl-p-tyrosine, p-tyramine induced a marked increase in the motor activity, which was not accompanied by a significant decrease in brain catecholamines, ruling out the possibility of indirect receptor stimulation. Since the presence of endogenous p-tyramine in the brain has been reported, these data may indicate a specific synaptic role for this amine, perhaps as a neurotransmitter.", "contents": "Release and receptor stimulating properties of p-tyramine in rat brain. Radiolabeled p-tyramine could be released from rat striatal slices, which were previously allowed to accumulate this amine, upon depolarization with media containing 40 mM potassium. This depolarization-induced release did not occur in the absence of calcium ions in the medium and over 75% of the labeled material released was identified chromatographically as tyramine. The possible existence of a direct receptor-stimulating activity of p-tyramine was examined by injecting this amine intraventricularly into rats, after pretreatment with various drugs, which inhibit catecholaminergic function, and measuring their motor activity. If injected into rats, which were pretreated with reserpine or alpha-methyl-p-tyrosine, p-tyramine induced a marked increase in the motor activity, which was not accompanied by a significant decrease in brain catecholamines, ruling out the possibility of indirect receptor stimulation. Since the presence of endogenous p-tyramine in the brain has been reported, these data may indicate a specific synaptic role for this amine, perhaps as a neurotransmitter.", "PMID": 952577}
{"id": "pubmed23n0032_71", "title": "Intravenous pharmacokinetic profile in rats of etomidate, a short-acting hypnotic drug.", "content": "Etomidate is a novel short-acting drug, whose pharmacokinetic profile was studied in 14 organs and tissues after i.v. administration to rats. Four dose levels, ranging from 224 to 1800 mug, were assayed between 1.75 and 112 min after administration. A three-compartment open linear model has been fitted to the plasma concentrations, assuming a central, peripheral and deep compartment. The fast component of the plasma curve, has a half-life of 1.19 min. Brain tissue rapidly enters in equilibrium with plasma and shows the highest concentration. Muscle, subcutaneous fat and possibly skin and connective tissues contribute most to the total amount of drug in the body. Many tissues are heterogeneous and exhibit combinations of central, peripheral and deep compartments. Elimination of etomidate occurs by ester-hydrolysis in plasma and in the liver with approximately equal rate constants. Metabolization of etomidate in the liver is a capacity-limited Michaelis-Menten process.", "contents": "Intravenous pharmacokinetic profile in rats of etomidate, a short-acting hypnotic drug. Etomidate is a novel short-acting drug, whose pharmacokinetic profile was studied in 14 organs and tissues after i.v. administration to rats. Four dose levels, ranging from 224 to 1800 mug, were assayed between 1.75 and 112 min after administration. A three-compartment open linear model has been fitted to the plasma concentrations, assuming a central, peripheral and deep compartment. The fast component of the plasma curve, has a half-life of 1.19 min. Brain tissue rapidly enters in equilibrium with plasma and shows the highest concentration. Muscle, subcutaneous fat and possibly skin and connective tissues contribute most to the total amount of drug in the body. Many tissues are heterogeneous and exhibit combinations of central, peripheral and deep compartments. Elimination of etomidate occurs by ester-hydrolysis in plasma and in the liver with approximately equal rate constants. Metabolization of etomidate in the liver is a capacity-limited Michaelis-Menten process.", "PMID": 952578}
{"id": "pubmed23n0032_72", "title": "The mechanism of contraction of rat aorta to various agonists.", "content": "To elucidate the mechanism of contraction of the smooth muscle of the rat's aorta, its response to norepinephrine (NE), 5-hydroxytrypatamine (5-HT) and potassium chloride (KCI) was determined before and after pretreatment with reserpine and beta-diethylamionethyl 2-2-diphenylpropyl acetate (SKF 525-A). Unlike the rabbit's aorta, contraction of the rat's aorta induced by NE was inhibited by SKF 525-A. After the induction of maximal contraction, SKF 525-A induced a graded rapid relaxation after KCl, less so after 5-HT, and least after NE. Pretreatment with reserpine failed to induce supersensitivity to NE. After incubation in a Ca++-free or Na+ and Ca++-free Krebs solution, the rat's aorta failed to contract even on the addition of Ca++ or NE.", "contents": "The mechanism of contraction of rat aorta to various agonists. To elucidate the mechanism of contraction of the smooth muscle of the rat's aorta, its response to norepinephrine (NE), 5-hydroxytrypatamine (5-HT) and potassium chloride (KCI) was determined before and after pretreatment with reserpine and beta-diethylamionethyl 2-2-diphenylpropyl acetate (SKF 525-A). Unlike the rabbit's aorta, contraction of the rat's aorta induced by NE was inhibited by SKF 525-A. After the induction of maximal contraction, SKF 525-A induced a graded rapid relaxation after KCl, less so after 5-HT, and least after NE. Pretreatment with reserpine failed to induce supersensitivity to NE. After incubation in a Ca++-free or Na+ and Ca++-free Krebs solution, the rat's aorta failed to contract even on the addition of Ca++ or NE.", "PMID": 952579}
{"id": "pubmed23n0032_73", "title": "The effect of temperature upon the positive inotropic and chronotropic responses of isolated cardiac preparations to isoprenaline and its modification by experimental variables.", "content": "The effects of various experimental variables upon the optimum temperatures for positive inotropic and chronotropic responses to isoprenaline in isolated cardiac preparations were examined. Perfusion rate and animal (heart) size affected the satisfactory determination of rate and tension optima of guinea-pig and rabbit perfused hearts. Isolated spontaneous atria were assumed to have the same temperature optima as perfused hearts and these were independent of the animal size/age. Paced atria, in which spontaneous rate changes were absent, revealed a sharper tension optimum at an unaltered temperature. The inotropic optimum in all preparations varied with the method of expressing the response; however, since the increase tension remained independent of resting developed tension in pacing experiments, this measure was adopted. The resulting inotropic and chronotropic optima, having minimized these variables, were 25 and 35, and 30 and 37.5 degrees C for guinea-pigs and rabbits respectively. This confirms the separation of these responses by temperature.", "contents": "The effect of temperature upon the positive inotropic and chronotropic responses of isolated cardiac preparations to isoprenaline and its modification by experimental variables. The effects of various experimental variables upon the optimum temperatures for positive inotropic and chronotropic responses to isoprenaline in isolated cardiac preparations were examined. Perfusion rate and animal (heart) size affected the satisfactory determination of rate and tension optima of guinea-pig and rabbit perfused hearts. Isolated spontaneous atria were assumed to have the same temperature optima as perfused hearts and these were independent of the animal size/age. Paced atria, in which spontaneous rate changes were absent, revealed a sharper tension optimum at an unaltered temperature. The inotropic optimum in all preparations varied with the method of expressing the response; however, since the increase tension remained independent of resting developed tension in pacing experiments, this measure was adopted. The resulting inotropic and chronotropic optima, having minimized these variables, were 25 and 35, and 30 and 37.5 degrees C for guinea-pigs and rabbits respectively. This confirms the separation of these responses by temperature.", "PMID": 952580}
{"id": "pubmed23n0032_74", "title": "Characteristics of responses of isolated human fallopian tube to transmural stimulation and to sympathomimetic amines.", "content": "The responses of circular and longitudinal muscle of human fallopian tube to transmural stimulation and sympathomimetic amines have been investigated. It was found that tissues from the earlier part of the menstrual cycle (i.e., estrogen dominant) were less responsive to all stimuli than those from the middle or late phase of the cycle. Although excitatory alpha responses of ampulla and isthmus could be observed, the predominant response of ampulla and isthmus, in all phases of the menstrual cycle, to (--)-noradrenaline and transmural stimulation was inhibition of spontaneous activity. The inhibitory response to transmural stimulation, was abolished by tetrodotoxin and guanethidine. The inhibitory responses to transmural stimulation and (--)-noradrenaline were reversed by propranolol. These findings show that the response of tissues to transmural stimulation resulted from release of noradrenaline from adrenergic nerves, and subsequent action on inhibitory beta adrenergic receptors.", "contents": "Characteristics of responses of isolated human fallopian tube to transmural stimulation and to sympathomimetic amines. The responses of circular and longitudinal muscle of human fallopian tube to transmural stimulation and sympathomimetic amines have been investigated. It was found that tissues from the earlier part of the menstrual cycle (i.e., estrogen dominant) were less responsive to all stimuli than those from the middle or late phase of the cycle. Although excitatory alpha responses of ampulla and isthmus could be observed, the predominant response of ampulla and isthmus, in all phases of the menstrual cycle, to (--)-noradrenaline and transmural stimulation was inhibition of spontaneous activity. The inhibitory response to transmural stimulation, was abolished by tetrodotoxin and guanethidine. The inhibitory responses to transmural stimulation and (--)-noradrenaline were reversed by propranolol. These findings show that the response of tissues to transmural stimulation resulted from release of noradrenaline from adrenergic nerves, and subsequent action on inhibitory beta adrenergic receptors.", "PMID": 952581}
{"id": "pubmed23n0032_75", "title": "The actions of ketamine on vascular smooth muscle.", "content": "The responses of rabbit aortic strips superfused with noradrenaline, adrenaline and 5-HT were studied alone and in combination with ketamine (50 mug/ml). Ketamine caused a slight depression of the isolated aorta but potentiated responses to adrenaline but not to noradrenaline or 5-hydroxytryptamine. Ketamine did not potentiate aortic strips contracted to a stable level by pyrogallol and adrenaline. Experiments carried out with COMT from homogenates of rat liver showed that, in contrast to pyrogallol (10(-5) M), ketamine (10(-3) M) did not inhibit the enzyme. Other experiments with rabbits given 6-hydroxydopamine showed that aortas of these rabbits responded in a similar manner to controls when treated with ketamine and catecholamines. Results obtained with aortas contracted by adrenaline and noradrenaline with ketamine present, followed by oil immersion, showed that ketamine prolonged greatly the relaxation induced by adrenaline and to a lesser extent the relaxation induced by noradrenaline. The results of these studies indicate that ketamine prevented catecholamines from reaching the intracellular site of COMT. In this respect, ketamine can be termed an inhibitor of uptake site 2. If this hypothesis is valid then the action of ketamine on vascular tissue might explain the cardiovascular effects of the drug in man and experimental animals.", "contents": "The actions of ketamine on vascular smooth muscle. The responses of rabbit aortic strips superfused with noradrenaline, adrenaline and 5-HT were studied alone and in combination with ketamine (50 mug/ml). Ketamine caused a slight depression of the isolated aorta but potentiated responses to adrenaline but not to noradrenaline or 5-hydroxytryptamine. Ketamine did not potentiate aortic strips contracted to a stable level by pyrogallol and adrenaline. Experiments carried out with COMT from homogenates of rat liver showed that, in contrast to pyrogallol (10(-5) M), ketamine (10(-3) M) did not inhibit the enzyme. Other experiments with rabbits given 6-hydroxydopamine showed that aortas of these rabbits responded in a similar manner to controls when treated with ketamine and catecholamines. Results obtained with aortas contracted by adrenaline and noradrenaline with ketamine present, followed by oil immersion, showed that ketamine prolonged greatly the relaxation induced by adrenaline and to a lesser extent the relaxation induced by noradrenaline. The results of these studies indicate that ketamine prevented catecholamines from reaching the intracellular site of COMT. In this respect, ketamine can be termed an inhibitor of uptake site 2. If this hypothesis is valid then the action of ketamine on vascular tissue might explain the cardiovascular effects of the drug in man and experimental animals.", "PMID": 952582}
{"id": "pubmed23n0032_76", "title": "Antitussive properties of butorphanol.", "content": "Butorphanol (levo-N-cyclobutylmethyl-3, 14-dihydroxy morphinan), a potent analgetic agent of the narcotic antagonist type with a low abuse potential in laboratory animals, was evaluated for antitussive activity in unanesthetized guinea-pigs and dogs. Subcutaneously, it was over 100 times more active than codeine, dextromethorphan and dl-pentazocine and about 20 times more active than morphine in the guinea-pig, while in the dog it was 100, 10 and 4 times more active than codeine, dl-pentazocine and morphine, respectively. Orally, butorphanol was 15-20 times more active than either codeine or dextromethrophan in both species. Naloxone reversed the antitussive effects of butorphanol, codeine, morphine and dl-pentazocine while those of dextromethorphan were not antagonized. The antitussive effect of butorphanol and morphine lasted about 4 hr and both compounds were longer acting than codeine. Butorphanol was also shown to be as effective against cough of pathological origin as against experimentally induced cough in the dog.", "contents": "Antitussive properties of butorphanol. Butorphanol (levo-N-cyclobutylmethyl-3, 14-dihydroxy morphinan), a potent analgetic agent of the narcotic antagonist type with a low abuse potential in laboratory animals, was evaluated for antitussive activity in unanesthetized guinea-pigs and dogs. Subcutaneously, it was over 100 times more active than codeine, dextromethorphan and dl-pentazocine and about 20 times more active than morphine in the guinea-pig, while in the dog it was 100, 10 and 4 times more active than codeine, dl-pentazocine and morphine, respectively. Orally, butorphanol was 15-20 times more active than either codeine or dextromethrophan in both species. Naloxone reversed the antitussive effects of butorphanol, codeine, morphine and dl-pentazocine while those of dextromethorphan were not antagonized. The antitussive effect of butorphanol and morphine lasted about 4 hr and both compounds were longer acting than codeine. Butorphanol was also shown to be as effective against cough of pathological origin as against experimentally induced cough in the dog.", "PMID": 952583}
{"id": "pubmed23n0032_77", "title": "A relatively simple differential screening test for GABA or glycine antagonists using rat electroretinography.", "content": "Intravitreal injection of GABA- and glycine-antagonists had specific and reversible effects on the ERG of rats. For example, picrotoxin induced characteristic rhythmic potentials. The effect was common to other GABA-antagonists, such as bicuculline, N-methylbicuculline and penicillin G. Strychnine caused the appearance of a- and c-like waves in response to low intensity flash stimuli in addition to the suppression of the b-wave commonly seen with agents such as asparate following higher intensity flash stimuli. The strychnine-like effect was common to other glycine-antagonist tested, such as brucine, morphine and laudanosine. The quanternization of the glycine-antagonists abolished the strychnine-like activity and endowed the ability to induce the rhythmic potentials characteristic of GABA-antagonists. This relatively simple method of observing either rhythmic potentials or a negative deflection (a-like wave) to a low intensity stimulus (about 1.0 log unit above dark threshold) in the ERG should be useful for the rapid screening of the predicted and new GABA--and glycine-antagonists.", "contents": "A relatively simple differential screening test for GABA or glycine antagonists using rat electroretinography. Intravitreal injection of GABA- and glycine-antagonists had specific and reversible effects on the ERG of rats. For example, picrotoxin induced characteristic rhythmic potentials. The effect was common to other GABA-antagonists, such as bicuculline, N-methylbicuculline and penicillin G. Strychnine caused the appearance of a- and c-like waves in response to low intensity flash stimuli in addition to the suppression of the b-wave commonly seen with agents such as asparate following higher intensity flash stimuli. The strychnine-like effect was common to other glycine-antagonist tested, such as brucine, morphine and laudanosine. The quanternization of the glycine-antagonists abolished the strychnine-like activity and endowed the ability to induce the rhythmic potentials characteristic of GABA-antagonists. This relatively simple method of observing either rhythmic potentials or a negative deflection (a-like wave) to a low intensity stimulus (about 1.0 log unit above dark threshold) in the ERG should be useful for the rapid screening of the predicted and new GABA--and glycine-antagonists.", "PMID": 952584}
{"id": "pubmed23n0032_78", "title": "Autoradiographic distribution study of 14C-DOPA in cat brain.", "content": "The penetration and distribution in various areas of cat brain of 14C-DL-DOPA and/or its derivatives was studied by the autoradiographic technique. The highest activity was found, 1 hr after administration, in the caudate nucleus, putamen and n. accumbens, while hypothalamus, substantia nigra, substantia grisea pericentralis, tuberculum olfactorium, raphe nuclei and nucleus interpeduncolaris showed lower levels of labelling. Very low activity was detected in the cerebral cortex and in the other gray and white matter structures of brain. After pretreatment with a peripheral DOPA-decarboxylase inhibitor or high dosage of cold DOPA, the distribution pattern was not modified but the levels of radioactivity were greatly enhanced. The localization of the drug in dopaminergic structures and other areas of the brain is discussed.", "contents": "Autoradiographic distribution study of 14C-DOPA in cat brain. The penetration and distribution in various areas of cat brain of 14C-DL-DOPA and/or its derivatives was studied by the autoradiographic technique. The highest activity was found, 1 hr after administration, in the caudate nucleus, putamen and n. accumbens, while hypothalamus, substantia nigra, substantia grisea pericentralis, tuberculum olfactorium, raphe nuclei and nucleus interpeduncolaris showed lower levels of labelling. Very low activity was detected in the cerebral cortex and in the other gray and white matter structures of brain. After pretreatment with a peripheral DOPA-decarboxylase inhibitor or high dosage of cold DOPA, the distribution pattern was not modified but the levels of radioactivity were greatly enhanced. The localization of the drug in dopaminergic structures and other areas of the brain is discussed.", "PMID": 952585}
{"id": "pubmed23n0032_79", "title": "Effects of DOCA on circulation, renal functions, body fluids and tissue electrolytes.", "content": "Desoxycorticosterone acetate (DOCA) was administered subcutaneously to 11 normal dogs (2 mg/kg/day) for 4 days. Few changes were observed in cardiovascular and renal functions except that the glomerular filtration rate was significantly increased. Total body water and plasma volume increased with a reduction of red blood cell volume. Hematocrit, plasma osmolality and total protein concentrations were decreased. The plasma K+ levels were lowered despite unchanged plasma concentrations of Na+, Ca++, Mg++, Cl- and phosphorus. The heart showed an increase in Na+ content and a decrease in K+, Ca++, phosphorus and total lipids. The skeletal muscle showed a decrease in Ca++ and an increase in Cl- and water content. The skin revealed a reduction of K+ and an elevation of Cl-. This study showed that a DOCA-induced depletion of K+ from skeletal muscle did not occur in normally fed dogs that did not receive a large sodium load.", "contents": "Effects of DOCA on circulation, renal functions, body fluids and tissue electrolytes. Desoxycorticosterone acetate (DOCA) was administered subcutaneously to 11 normal dogs (2 mg/kg/day) for 4 days. Few changes were observed in cardiovascular and renal functions except that the glomerular filtration rate was significantly increased. Total body water and plasma volume increased with a reduction of red blood cell volume. Hematocrit, plasma osmolality and total protein concentrations were decreased. The plasma K+ levels were lowered despite unchanged plasma concentrations of Na+, Ca++, Mg++, Cl- and phosphorus. The heart showed an increase in Na+ content and a decrease in K+, Ca++, phosphorus and total lipids. The skeletal muscle showed a decrease in Ca++ and an increase in Cl- and water content. The skin revealed a reduction of K+ and an elevation of Cl-. This study showed that a DOCA-induced depletion of K+ from skeletal muscle did not occur in normally fed dogs that did not receive a large sodium load.", "PMID": 952586}
{"id": "pubmed23n0032_80", "title": "Discrimination of narcotic drugs on 3H-fentanyl receptor binding.", "content": "Stereospecific opiate receptor binding was measured using specifically labeled fentanyl in the presence of an excess of either dextromoramide or levomoramide, only the former being the analgesic isomer. In a very large series of chemically related drugs, but of different pharmacological activity, it was possible to recognize compounds endowed with a potential morphine-like activity. Attempts were made to localize the opiate receptors within the neuronal cell.", "contents": "Discrimination of narcotic drugs on 3H-fentanyl receptor binding. Stereospecific opiate receptor binding was measured using specifically labeled fentanyl in the presence of an excess of either dextromoramide or levomoramide, only the former being the analgesic isomer. In a very large series of chemically related drugs, but of different pharmacological activity, it was possible to recognize compounds endowed with a potential morphine-like activity. Attempts were made to localize the opiate receptors within the neuronal cell.", "PMID": 952589}
{"id": "pubmed23n0032_81", "title": "Effect of isoproterenol on urinary function in goats.", "content": "Intraventricular administration of small doses (6.3-19-58 ng/kg/min) of isoproterenol (ISO) in non-hydrated goats produced a diuretic response, a decrease in urinary Na+, K+ and Cl- excretion and an increase in free water clearance. The diuretic response of ISO could be blocked by propranolol, but not by phentolamine. The data suggest that hypothalamic beta-adrenoceptors are involved in the inhibition of ADH release.", "contents": "Effect of isoproterenol on urinary function in goats. Intraventricular administration of small doses (6.3-19-58 ng/kg/min) of isoproterenol (ISO) in non-hydrated goats produced a diuretic response, a decrease in urinary Na+, K+ and Cl- excretion and an increase in free water clearance. The diuretic response of ISO could be blocked by propranolol, but not by phentolamine. The data suggest that hypothalamic beta-adrenoceptors are involved in the inhibition of ADH release.", "PMID": 952590}
{"id": "pubmed23n0032_82", "title": "[Results of Mustard's operation for transposition of the great vessels. Apropos of 81 cases of children who survived the intervention with a follow-up of from 1 to 6 years].", "content": "In a series of 81 children who survived to Mustard's operation, with a follow-up varying from 6 months to 6 years, there were 19% of secondary deaths, 19% of bad results, 29% of questionable results and only 33% of good results. The most frequent complications were: disturbances of cardiac rhythm and obstructions to pulmonary or caval venous return.", "contents": "[Results of Mustard's operation for transposition of the great vessels. Apropos of 81 cases of children who survived the intervention with a follow-up of from 1 to 6 years]. In a series of 81 children who survived to Mustard's operation, with a follow-up varying from 6 months to 6 years, there were 19% of secondary deaths, 19% of bad results, 29% of questionable results and only 33% of good results. The most frequent complications were: disturbances of cardiac rhythm and obstructions to pulmonary or caval venous return.", "PMID": 952593}
{"id": "pubmed23n0032_83", "title": "[Evaluation of gonadotropic and gonadal secretions in primary abnormalities of the gonads and male pseudohermaphrodism before and after the age of puberty].", "content": "Blood levels of gonadotropins (FSH and LH) and gonadal steroids (testosterone or estradiol) have been evaluated in 72 children and adolescents with primary gonadal defects (45 agonadal patients, 7 with asymetrical gonadal dysgenesis, 9 with Klinefelter syndrome, 4 with partial testicular dysgenesis and 7 with partial ovarian dysgenesis) and in 17 cases of male pseudohermaphrodism. A LH-RH test has been performed in most cases, and a testicular stimulation test with chorionic gonadotropin in patients with testicular tissue. Agonadal subjects had increased blood and pituitary releasable gonadotropins: very high in infants and young children, much less from 7 to 11 years, a high spurt being observed at 12 years. This diphasic pattern relates to the varying sensitivity of hypothalamic receptors and suggests that adrenal steroids may restrain gonadotropic secretion at 7-8 years. As a diagnostic tool, the increase of gonadotropins may be missing in the 7-11 years group. In the 1-12 years patients with partially defective gonads, blood and pituitary releasable gonadotropins and blood steroids are usually normal, but testosterone response to chorionic gonadotropin may be already blunted. From 13 years the gonadotropic secretion is usually increased, even when testosterone secretion and reserve are within normal range. Most male pseudohermaphrodites showed normal gonadotropic and testicular secretions. But four patients had highly increased responses to LH-RH, suggesting a defect of testicular secretion or of receptors, and demonstrating some heterogeneity in the male pseudohermaphrodism group.", "contents": "[Evaluation of gonadotropic and gonadal secretions in primary abnormalities of the gonads and male pseudohermaphrodism before and after the age of puberty]. Blood levels of gonadotropins (FSH and LH) and gonadal steroids (testosterone or estradiol) have been evaluated in 72 children and adolescents with primary gonadal defects (45 agonadal patients, 7 with asymetrical gonadal dysgenesis, 9 with Klinefelter syndrome, 4 with partial testicular dysgenesis and 7 with partial ovarian dysgenesis) and in 17 cases of male pseudohermaphrodism. A LH-RH test has been performed in most cases, and a testicular stimulation test with chorionic gonadotropin in patients with testicular tissue. Agonadal subjects had increased blood and pituitary releasable gonadotropins: very high in infants and young children, much less from 7 to 11 years, a high spurt being observed at 12 years. This diphasic pattern relates to the varying sensitivity of hypothalamic receptors and suggests that adrenal steroids may restrain gonadotropic secretion at 7-8 years. As a diagnostic tool, the increase of gonadotropins may be missing in the 7-11 years group. In the 1-12 years patients with partially defective gonads, blood and pituitary releasable gonadotropins and blood steroids are usually normal, but testosterone response to chorionic gonadotropin may be already blunted. From 13 years the gonadotropic secretion is usually increased, even when testosterone secretion and reserve are within normal range. Most male pseudohermaphrodites showed normal gonadotropic and testicular secretions. But four patients had highly increased responses to LH-RH, suggesting a defect of testicular secretion or of receptors, and demonstrating some heterogeneity in the male pseudohermaphrodism group.", "PMID": 952594}
{"id": "pubmed23n0032_84", "title": "[Pediatric pharmacology. Practical ideas of kinetics and metabolism of drugs].", "content": "After administration, a drug does not exert immediately all its actions. Rates of diffusion, fixation and excretion of drugs are depending from their nature as well as from the condition of the body to which they are given. The resultant of these mechanisms control the blood level of a drug, level which varies in function of time. The blood level reflects the pharmacodynamic activity of a drug. Information on kinetics and significance of the status of drug equilibrium are of major importance for rational drug administration.", "contents": "[Pediatric pharmacology. Practical ideas of kinetics and metabolism of drugs]. After administration, a drug does not exert immediately all its actions. Rates of diffusion, fixation and excretion of drugs are depending from their nature as well as from the condition of the body to which they are given. The resultant of these mechanisms control the blood level of a drug, level which varies in function of time. The blood level reflects the pharmacodynamic activity of a drug. Information on kinetics and significance of the status of drug equilibrium are of major importance for rational drug administration.", "PMID": 952595}
{"id": "pubmed23n0032_85", "title": "[Attempts at a nosological classification with two standardized psychiatric rating scales (author's transl)].", "content": "Three different procedures for classification of psychiatric patients, a modified form of Q factor analysis, the classical discriminant analysis and a simple procedure based on clinical concepts, were compared empirically. On the basis of the psychopathological state rated by means of the Inpatient Multidimensional Psychiatric Scale (IMPS) and psychopathological and somatic scales of the AMP documentation system (AMP scale), the three procedures were applied in order to reproduce the psychiatric diagnosis. The classification was made by paired comparison of the following four diagnoses: schizophrenia, paranoid form (n = 45), schizophrenia, unspecified form (n = 47), depressive psychosis (n = 44), and depressive neurosis (n = 53). The procedures were evaluated according to their crossvalidation results. The mean percentages of correct classification were the following: 83% for the modified form of Q factor analysis, 77% for the classical discriminant analysis, 73% for the simple procedure based on clinical concepts using the IMPS, and 79% for the same procedure, but using the AMP scale, all three procedures reaching about the same level of validity.", "contents": "[Attempts at a nosological classification with two standardized psychiatric rating scales (author's transl)]. Three different procedures for classification of psychiatric patients, a modified form of Q factor analysis, the classical discriminant analysis and a simple procedure based on clinical concepts, were compared empirically. On the basis of the psychopathological state rated by means of the Inpatient Multidimensional Psychiatric Scale (IMPS) and psychopathological and somatic scales of the AMP documentation system (AMP scale), the three procedures were applied in order to reproduce the psychiatric diagnosis. The classification was made by paired comparison of the following four diagnoses: schizophrenia, paranoid form (n = 45), schizophrenia, unspecified form (n = 47), depressive psychosis (n = 44), and depressive neurosis (n = 53). The procedures were evaluated according to their crossvalidation results. The mean percentages of correct classification were the following: 83% for the modified form of Q factor analysis, 77% for the classical discriminant analysis, 73% for the simple procedure based on clinical concepts using the IMPS, and 79% for the same procedure, but using the AMP scale, all three procedures reaching about the same level of validity.", "PMID": 952596}
{"id": "pubmed23n0032_86", "title": "[Multiple sclerosis and personality aspects (author's transl)].", "content": "In a sample of 85 multiple sclerosis patients an increase of the objective neurological degree of severity of the illness resulted in a significant decrease of manifold psychometrically recorded functions of intelligence (verbal and non-verbal intelligence). Of the personality characteristics registered by questionnaire only depression proved significant, but numerically slightly positive correlation with the degree of severity of the illness. The correlations between degree of severity and personality characteristics, as aggressiveness, sociability, neuroticism, extraversion were not significant. The personality-psychological results were especially interpreted and discussed with regard to the therapeutic consequences.", "contents": "[Multiple sclerosis and personality aspects (author's transl)]. In a sample of 85 multiple sclerosis patients an increase of the objective neurological degree of severity of the illness resulted in a significant decrease of manifold psychometrically recorded functions of intelligence (verbal and non-verbal intelligence). Of the personality characteristics registered by questionnaire only depression proved significant, but numerically slightly positive correlation with the degree of severity of the illness. The correlations between degree of severity and personality characteristics, as aggressiveness, sociability, neuroticism, extraversion were not significant. The personality-psychological results were especially interpreted and discussed with regard to the therapeutic consequences.", "PMID": 952597}
{"id": "pubmed23n0032_87", "title": "[Investigations of fasting blood glucose in endogenous depressive, schizophrenic, and neurotic patients (author's transl)].", "content": "Fasting blood glucose was estimated in endogenous depressive, schizophrenic, and neurotic patients. Mean fasting blood glucose levels were found to be significantly increased in endogenous depressive patients as compared with schizophrenics and neurotics. This finding is thought to be a sensitive symptom for a decrease in glucose tolerance in endogenous depression. No correlation could be detected to sex, body weight: body height ratio or age. Several hypothetical explanations of the finding are discussed.", "contents": "[Investigations of fasting blood glucose in endogenous depressive, schizophrenic, and neurotic patients (author's transl)]. Fasting blood glucose was estimated in endogenous depressive, schizophrenic, and neurotic patients. Mean fasting blood glucose levels were found to be significantly increased in endogenous depressive patients as compared with schizophrenics and neurotics. This finding is thought to be a sensitive symptom for a decrease in glucose tolerance in endogenous depression. No correlation could be detected to sex, body weight: body height ratio or age. Several hypothetical explanations of the finding are discussed.", "PMID": 952598}
{"id": "pubmed23n0032_88", "title": "[Investigation of the interraterreliability of the AMP system (author's transl)].", "content": "The interraterreliability of the AMP system (developed by the Arbeitsgemeinschaft f\u00fcr Methodik und Kokumentation in der Psychiatrie (AMP)) has been studied for the first time concerning the level of scales. The investigation was carried out using 80 ratings from two raters concerning a group of acute schizophrenic patients. The results pointed out the extreme usefulness of the AMP system also with respect to interraterreliability. Furthermore, a comparison between the group studied (ICD 295) and a group of schizophrenic patients (ICD 295) from the literature indicated a special AMP profile for groups of schizophrenic patients, the paranoid subgroup (295.3) being predominant.", "contents": "[Investigation of the interraterreliability of the AMP system (author's transl)]. The interraterreliability of the AMP system (developed by the Arbeitsgemeinschaft f\u00fcr Methodik und Kokumentation in der Psychiatrie (AMP)) has been studied for the first time concerning the level of scales. The investigation was carried out using 80 ratings from two raters concerning a group of acute schizophrenic patients. The results pointed out the extreme usefulness of the AMP system also with respect to interraterreliability. Furthermore, a comparison between the group studied (ICD 295) and a group of schizophrenic patients (ICD 295) from the literature indicated a special AMP profile for groups of schizophrenic patients, the paranoid subgroup (295.3) being predominant.", "PMID": 952599}
{"id": "pubmed23n0032_89", "title": "[Controlled EEG alpha feedback training in normals and headache patients (author's transl)].", "content": "Headache patients and healthy controls underwent alpha-EEG feedback training in 12 sessions. The present study does not support the literature which reports alpha increase by feedback training. The study included a patient group receiving feedback, a patient control group receiving pseudofeedback, and a volunteer group receiving feedback. Increase of alpha EEG was observed under feedback and pseudofeedback. However, there was always more alpha during baseline times than during training times. Alpha increase over time is suggested to be a habituation effect. Headache pain decreased with training (within the boundaries of a placebo effect), and there was no difference in headache decrease between feedback and pseudofeedback patients.", "contents": "[Controlled EEG alpha feedback training in normals and headache patients (author's transl)]. Headache patients and healthy controls underwent alpha-EEG feedback training in 12 sessions. The present study does not support the literature which reports alpha increase by feedback training. The study included a patient group receiving feedback, a patient control group receiving pseudofeedback, and a volunteer group receiving feedback. Increase of alpha EEG was observed under feedback and pseudofeedback. However, there was always more alpha during baseline times than during training times. Alpha increase over time is suggested to be a habituation effect. Headache pain decreased with training (within the boundaries of a placebo effect), and there was no difference in headache decrease between feedback and pseudofeedback patients.", "PMID": 952600}
{"id": "pubmed23n0032_90", "title": "[How alcohol and drug consumers see themselves: a personal and social self-assessment (author's transl)].", "content": "In a representative inquiry we found significant differences in the self-assessment of 4082 20-year-old Swiss men, as to whether their consumption of alcohol and drugs is high, low, or zero. The heavy users of alcohol and drugs describe themselves as men with frequent personal difficulties and psychosomatic disturbances. They tend to separate earlier from their family and prefer a less conventional, more consume-orientated, and passive way of life than the remainder.", "contents": "[How alcohol and drug consumers see themselves: a personal and social self-assessment (author's transl)]. In a representative inquiry we found significant differences in the self-assessment of 4082 20-year-old Swiss men, as to whether their consumption of alcohol and drugs is high, low, or zero. The heavy users of alcohol and drugs describe themselves as men with frequent personal difficulties and psychosomatic disturbances. They tend to separate earlier from their family and prefer a less conventional, more consume-orientated, and passive way of life than the remainder.", "PMID": 952601}
{"id": "pubmed23n0032_91", "title": "[On the concept of \"situation\" in psychopathology (author's transl)].", "content": "In continuation of previous work, the present investigation attempts to present the concept of situation from the point of view of an interactional psychopatholgy. Three aspects of situation are emphasized, the impressive, the expressive, and the dynamic. Some kinds of psychopathologically significant situation-changes are described as examples.", "contents": "[On the concept of \"situation\" in psychopathology (author's transl)]. In continuation of previous work, the present investigation attempts to present the concept of situation from the point of view of an interactional psychopatholgy. Three aspects of situation are emphasized, the impressive, the expressive, and the dynamic. Some kinds of psychopathologically significant situation-changes are described as examples.", "PMID": 952602}
{"id": "pubmed23n0032_92", "title": "[Critical remarks on the symptom of \"thought insertion\" (author's transl)].", "content": "\"Thought insertion\" is one of Kurt Schneider's first rank symptoms of schizophrenia. Earlier, Karl Jaspers had made a finer distinction, differentiating between \"thought insertion in a narrower sense\" and \"made thoughts.\" However, most German and English writers have dispensed with the Jasperian criteria for this differentiation and consequently their use of the term \"thought insertion\" is broader. Such authors, including Kurt Schneider and Weitbrecht, usually speak of \"thought insertion\", \"made thoughts\", and \"influencing of thoughts\" as if they were identical phenomena. In contrast, Jaspers' differentiation offers us the possibility of more clearly distinguishing such symptoms and thus his distinction should not be neglected. Furthermore, the Jasperian criteria can be logically and usefully extended to all \"made and passivity experiences\" enabling one to recognise four aspects of such phenomena. Some general differential diagnostic considerations with respect to \"thought insertion\" are then presented.", "contents": "[Critical remarks on the symptom of \"thought insertion\" (author's transl)]. \"Thought insertion\" is one of Kurt Schneider's first rank symptoms of schizophrenia. Earlier, Karl Jaspers had made a finer distinction, differentiating between \"thought insertion in a narrower sense\" and \"made thoughts.\" However, most German and English writers have dispensed with the Jasperian criteria for this differentiation and consequently their use of the term \"thought insertion\" is broader. Such authors, including Kurt Schneider and Weitbrecht, usually speak of \"thought insertion\", \"made thoughts\", and \"influencing of thoughts\" as if they were identical phenomena. In contrast, Jaspers' differentiation offers us the possibility of more clearly distinguishing such symptoms and thus his distinction should not be neglected. Furthermore, the Jasperian criteria can be logically and usefully extended to all \"made and passivity experiences\" enabling one to recognise four aspects of such phenomena. Some general differential diagnostic considerations with respect to \"thought insertion\" are then presented.", "PMID": 952603}
{"id": "pubmed23n0032_93", "title": "Electroencephalographic laterality changes during human sexual orgasm.", "content": "Left and right parietal EEGs were recorded while seven subjects experienced sexual climax through self-stimulation. EEG data were quantified by continuous cumulated measurements of the integrated areas of EEG recording during successive 1-sec epochs. In eight out of 12 experiments, this measure revealed a statistically significant change in laterality. Controlled replications with two of the original subjects obviated the possibility that two potential sources of artifact (hand used for masturbation and gross body movements) accounted for the changes. While previous research has focused on vasomotor and myotonic indices of sexual response, this study demonstrates the significance of brain phenomena in sexual orgasm.", "contents": "Electroencephalographic laterality changes during human sexual orgasm. Left and right parietal EEGs were recorded while seven subjects experienced sexual climax through self-stimulation. EEG data were quantified by continuous cumulated measurements of the integrated areas of EEG recording during successive 1-sec epochs. In eight out of 12 experiments, this measure revealed a statistically significant change in laterality. Controlled replications with two of the original subjects obviated the possibility that two potential sources of artifact (hand used for masturbation and gross body movements) accounted for the changes. While previous research has focused on vasomotor and myotonic indices of sexual response, this study demonstrates the significance of brain phenomena in sexual orgasm.", "PMID": 952604}
{"id": "pubmed23n0032_94", "title": "Polygyny: insufficient father-son contact and son's masculine identity.", "content": "In polygynous societies in which the family consists of husband, wives, and children only, if each cowife lives in a separate quarter with her children, males are more likely to be circumcised or segregated at puberty. These customs are interpreted as the measures to rectify boys'  mother-oriented personality development because of limited contact with their fathers due to their mother's separate quarters. Circumcision and segregation can be explained better in terms of the son's insufficient contact with the father rather than a very close relationship with the mother due to the long postpartum sexual taboo.", "contents": "Polygyny: insufficient father-son contact and son's masculine identity. In polygynous societies in which the family consists of husband, wives, and children only, if each cowife lives in a separate quarter with her children, males are more likely to be circumcised or segregated at puberty. These customs are interpreted as the measures to rectify boys'  mother-oriented personality development because of limited contact with their fathers due to their mother's separate quarters. Circumcision and segregation can be explained better in terms of the son's insufficient contact with the father rather than a very close relationship with the mother due to the long postpartum sexual taboo.", "PMID": 952605}
{"id": "pubmed23n0032_95", "title": "Autonomic responses of transsexual and homosexual males to erotic film sequences.", "content": "Penile volume and galvanic skin responses to nude female and male film sequences were studied in 10 transsexual patients, 44 patients requesting treatment for homosexual impulses, and 60 heterosexual students. Student controls and homosexuals showed significantly greater galvanic skin responses to the preferred than to the nonpreferred sex. Transsexuals tended to show larger galvanic skin responses to females than did male homosexuals. No strong relationships were found between penile volume and galvanic skin response to the preferred sex. It is concluded that transsexual patients differ significantly from homosexual patients in autonomic responsivity, which may have diagnostic usefulness.", "contents": "Autonomic responses of transsexual and homosexual males to erotic film sequences. Penile volume and galvanic skin responses to nude female and male film sequences were studied in 10 transsexual patients, 44 patients requesting treatment for homosexual impulses, and 60 heterosexual students. Student controls and homosexuals showed significantly greater galvanic skin responses to the preferred than to the nonpreferred sex. Transsexuals tended to show larger galvanic skin responses to females than did male homosexuals. No strong relationships were found between penile volume and galvanic skin response to the preferred sex. It is concluded that transsexual patients differ significantly from homosexual patients in autonomic responsivity, which may have diagnostic usefulness.", "PMID": 952606}
{"id": "pubmed23n0032_96", "title": "Alternative behavioral approaches to the treatment of homosexuality.", "content": "Radical changes in attitudes toward homosexuality in American society are integrated into a new perspective for the therapist confronted by a client who engages in homosexual behavior. The traditional mandatory attempt to eradicate homosexual behavior has been expanded into three options, any one of which may be pursued by the therapist at the client's request: (1) modification of homosexual in favor of heterosexual behavior, (2) enhancement of homosexual behavior, and (3) ignoring of homosexual behavior if it is functionally unrelated to the presenting symptoms. Therapists' negative attitudes toward clients who engage in homosexual behavior are such as to abrogate expanded options and may results in therapeutic efforts at modification of homosexual behavior in defiance of the client's expressed wishes. Contrary to current professional belief, careful analysis and the use of appropriate techniques such as systematic desensitization, orgasmic reconditioning, and training in heterosocial skills generally obviate the necessity for aversive procedures in those instances where homosexual behavior is to be modified in favor of heterosexual behavior. Three case histories are presented illustrating the use of each of the expanded options described above.", "contents": "Alternative behavioral approaches to the treatment of homosexuality. Radical changes in attitudes toward homosexuality in American society are integrated into a new perspective for the therapist confronted by a client who engages in homosexual behavior. The traditional mandatory attempt to eradicate homosexual behavior has been expanded into three options, any one of which may be pursued by the therapist at the client's request: (1) modification of homosexual in favor of heterosexual behavior, (2) enhancement of homosexual behavior, and (3) ignoring of homosexual behavior if it is functionally unrelated to the presenting symptoms. Therapists' negative attitudes toward clients who engage in homosexual behavior are such as to abrogate expanded options and may results in therapeutic efforts at modification of homosexual behavior in defiance of the client's expressed wishes. Contrary to current professional belief, careful analysis and the use of appropriate techniques such as systematic desensitization, orgasmic reconditioning, and training in heterosocial skills generally obviate the necessity for aversive procedures in those instances where homosexual behavior is to be modified in favor of heterosexual behavior. Three case histories are presented illustrating the use of each of the expanded options described above.", "PMID": 952607}
{"id": "pubmed23n0032_97", "title": "Atttitudes of the aged toward nontraditional sexual behavior.", "content": "Age as a correlate of attitudes toward nontraditional sexual behavior is explored. The data were drawn from a United State national probability sample. Subgroup comparisons were made between two age cohorts--respondents over and under 65. Sexual attitudes were measured toward premarital sexual relations, extramartial sexual relations, and homosexuality. Older respondents held the most conservative sexual attitudes. However, the background variables of sex, social status, church attendance, marital status, and parenthood were predictors of sexual attitudes for both age strata. The findings indicate individual variations among the aged with the likelihood of a greater range of differences in the future.", "contents": "Atttitudes of the aged toward nontraditional sexual behavior. Age as a correlate of attitudes toward nontraditional sexual behavior is explored. The data were drawn from a United State national probability sample. Subgroup comparisons were made between two age cohorts--respondents over and under 65. Sexual attitudes were measured toward premarital sexual relations, extramartial sexual relations, and homosexuality. Older respondents held the most conservative sexual attitudes. However, the background variables of sex, social status, church attendance, marital status, and parenthood were predictors of sexual attitudes for both age strata. The findings indicate individual variations among the aged with the likelihood of a greater range of differences in the future.", "PMID": 952608}
{"id": "pubmed23n0032_98", "title": "A law concerning sex reassignment of transsexuals in Sweden.", "content": "In 1972, a law regulating surgical and legal measures reguired for sex reassignment came into force in Sweden. The law, its practical application, and the number of transsexual cases dealt with by the National Board of Health and Welfare during the first 2 years of enforcement are described.", "contents": "A law concerning sex reassignment of transsexuals in Sweden. In 1972, a law regulating surgical and legal measures reguired for sex reassignment came into force in Sweden. The law, its practical application, and the number of transsexual cases dealt with by the National Board of Health and Welfare during the first 2 years of enforcement are described.", "PMID": 952609}
{"id": "pubmed23n0032_99", "title": "Combined intervention for controlling unwanted homosexual behavior: an extended follow-up.", "content": "Of 49 patients who were able via previous therapy to control their sexual arousal in the presence of homosexual stimuli, 31 (63%) were contracted for follow-up. The average period since the end of treatment was 4 years. Nineteen subjects (61%) have remained exclusively heterosexual, whereas nine (29%) have had homosexual intercourse. Heterosexual intercourse was reported in 28 (90%), including the previous nine subjects. Three (10%) subjects have had neither homonor heterosexual intercourse.", "contents": "Combined intervention for controlling unwanted homosexual behavior: an extended follow-up. Of 49 patients who were able via previous therapy to control their sexual arousal in the presence of homosexual stimuli, 31 (63%) were contracted for follow-up. The average period since the end of treatment was 4 years. Nineteen subjects (61%) have remained exclusively heterosexual, whereas nine (29%) have had homosexual intercourse. Heterosexual intercourse was reported in 28 (90%), including the previous nine subjects. Three (10%) subjects have had neither homonor heterosexual intercourse.", "PMID": 952610}
{"id": "pubmed23n0032_100", "title": "Effect of modeling on sexual imagery.", "content": "Social learning theory was used to examine the effects of a model's sexual imagery on the observer's sexual imagery. In the guise of a creative writing experiment, male and female college students were asked to listen to a tape recording of a same- or opposite-sex model relating a story in response to a sample TAT card. The story described a man and a woman in a physical sex encounter (high sex), a romantic date (medium sex), or a casual study date (low sex). The sample TAT picture and model's story were omitted in the control groups. All subjects wrote stories in response to two other TAT cards. These stories were scored for sexual imagery by a male and a female judge who were blind to experimental conditions and who used a standard sexual imagery scoring manual. The following prediction were based on social learning theory: There would be greater sexual imagery in the stories of subjects who heard the high sex model than in the stories of those who heard the medium or low sex model or no model. Past research implied the prediction that the modeling effects would be greater for males than for females in the high sex model condition and greater for females than for males in the medium sex model condition. The results were analyzed using two factorial analyses of variance. There was greater sexual imagery by subjects who heard the high sex model than by those who heard the low sex model or model. The sexual imagery by subjects who heard the medium sex model was intermediate between that by those who heard the high sex model and that by those who heard the low sex model. The modeling effect was greater in males. The results also confirmed the prediction that sexual imagery would be greater for males in the high sex model condition but did not confirm the prediction that sexual imagery would be greater for females in the medium sex model condition.", "contents": "Effect of modeling on sexual imagery. Social learning theory was used to examine the effects of a model's sexual imagery on the observer's sexual imagery. In the guise of a creative writing experiment, male and female college students were asked to listen to a tape recording of a same- or opposite-sex model relating a story in response to a sample TAT card. The story described a man and a woman in a physical sex encounter (high sex), a romantic date (medium sex), or a casual study date (low sex). The sample TAT picture and model's story were omitted in the control groups. All subjects wrote stories in response to two other TAT cards. These stories were scored for sexual imagery by a male and a female judge who were blind to experimental conditions and who used a standard sexual imagery scoring manual. The following prediction were based on social learning theory: There would be greater sexual imagery in the stories of subjects who heard the high sex model than in the stories of those who heard the medium or low sex model or no model. Past research implied the prediction that the modeling effects would be greater for males than for females in the high sex model condition and greater for females than for males in the medium sex model condition. The results were analyzed using two factorial analyses of variance. There was greater sexual imagery by subjects who heard the high sex model than by those who heard the low sex model or model. The sexual imagery by subjects who heard the medium sex model was intermediate between that by those who heard the high sex model and that by those who heard the low sex model. The modeling effect was greater in males. The results also confirmed the prediction that sexual imagery would be greater for males in the high sex model condition but did not confirm the prediction that sexual imagery would be greater for females in the medium sex model condition.", "PMID": 952611}
{"id": "pubmed23n0032_101", "title": "Follow-up effects of a medical school course in human sexuality.", "content": "The effects of a course in human sexuality for a class of second-year male medical students are described. The course consists of two interacting components: a 2-day seminar and a 2-week didactic presentation. The purpose of the seminar is to develop humanistic understanding and tolerance of one's own and others' sexuality. The didactic presentation focuses on factual information. A variety of evaluation instruments were used, and students were tested precourse, postcourse, and at the end of 6 and 12 months. Results show a significant pre-post increase in tolerance in attitude and amount of knowledge. There appears to be some moderation in level of attitude and knowledge gain at the 6-months point, and significant increases from the precourse level to the 12-months point persist. Participants almost uniformly report personal benefit, with a significant number described greater satisfaction in their own sexual behaviour.", "contents": "Follow-up effects of a medical school course in human sexuality. The effects of a course in human sexuality for a class of second-year male medical students are described. The course consists of two interacting components: a 2-day seminar and a 2-week didactic presentation. The purpose of the seminar is to develop humanistic understanding and tolerance of one's own and others' sexuality. The didactic presentation focuses on factual information. A variety of evaluation instruments were used, and students were tested precourse, postcourse, and at the end of 6 and 12 months. Results show a significant pre-post increase in tolerance in attitude and amount of knowledge. There appears to be some moderation in level of attitude and knowledge gain at the 6-months point, and significant increases from the precourse level to the 12-months point persist. Participants almost uniformly report personal benefit, with a significant number described greater satisfaction in their own sexual behaviour.", "PMID": 952612}
{"id": "pubmed23n0032_102", "title": "Group treatment format: mixed sexual dysfunctions.", "content": "This study reports the rationale and results of a ten-session group composed os six couples with mixed sexual dysfunctions. Dysfunctions included premature ejaculation, primary and situational orgasmic dysfunction, situational impotence, and incompatible sex drives. A variety of pretreatment behavioral and attitudinal measures were administered, including a Life History Questionaire, Sexual Assessment Inventory, Marital Adjustment Scale, and Sexual Interaction, orgasmic attainment, ejaculatory control, and enhanced self-acceptance, particularly for females (SII changes). The consistency between outcome measures was also a noteworthy finding. The results suggest increased use of mixed short-term groups, particularly when cost-effectiveness accountability is important. One contraindication for such groups is, however, severe marital discord, as evidenced by Locke-Wallace mean scores of 80 or less.", "contents": "Group treatment format: mixed sexual dysfunctions. This study reports the rationale and results of a ten-session group composed os six couples with mixed sexual dysfunctions. Dysfunctions included premature ejaculation, primary and situational orgasmic dysfunction, situational impotence, and incompatible sex drives. A variety of pretreatment behavioral and attitudinal measures were administered, including a Life History Questionaire, Sexual Assessment Inventory, Marital Adjustment Scale, and Sexual Interaction, orgasmic attainment, ejaculatory control, and enhanced self-acceptance, particularly for females (SII changes). The consistency between outcome measures was also a noteworthy finding. The results suggest increased use of mixed short-term groups, particularly when cost-effectiveness accountability is important. One contraindication for such groups is, however, severe marital discord, as evidenced by Locke-Wallace mean scores of 80 or less.", "PMID": 952613}
{"id": "pubmed23n0032_103", "title": "Homosexuality: behaviours and attitudes.", "content": "To assess the incidence of homosexual experiences, heterosexual experiences, associated attitudes, and related demographic characteristics, a sexual behaviour and attitudes questionaire was administered to 4251 university students. The rate of homosexual experiences was found to be appreciably lower than that commonly reported in the literature and was not significantly related to race, religion, or region of residence. Individuals with homosexual experiences, compared to those without, tended to be more liberal in their views toward socially deviant sexual behavior in general, did not demonstrate fewer heterosexual contacts, and did not report significantly more guilt or anxiety about their sexual behavior.", "contents": "Homosexuality: behaviours and attitudes. To assess the incidence of homosexual experiences, heterosexual experiences, associated attitudes, and related demographic characteristics, a sexual behaviour and attitudes questionaire was administered to 4251 university students. The rate of homosexual experiences was found to be appreciably lower than that commonly reported in the literature and was not significantly related to race, religion, or region of residence. Individuals with homosexual experiences, compared to those without, tended to be more liberal in their views toward socially deviant sexual behavior in general, did not demonstrate fewer heterosexual contacts, and did not report significantly more guilt or anxiety about their sexual behavior.", "PMID": 952614}
{"id": "pubmed23n0032_104", "title": "The intelligence of rapists.", "content": "IQs of rapists were compared to IQs of nonrapist prisoners convicted of violent crimes and nonrapist prisoners convicted of nonviolent crimes. Subjects were 136 male convicted felons in the Kentucky State Penitentiary. Determination of violent vs. nonviolent crimes other than rape was made upon consultation with a Kentucky Commonwealth Attorney. The results revealed significantly lower IQs for rapists compared to nonrapists convicted of violent crimes and compared to heterogeneous nonrapist convicts. The present research supports the findings of Karacan and co-workers, indicating a general tendency for rapists to have lower IQs than other convicted prisoners.", "contents": "The intelligence of rapists. IQs of rapists were compared to IQs of nonrapist prisoners convicted of violent crimes and nonrapist prisoners convicted of nonviolent crimes. Subjects were 136 male convicted felons in the Kentucky State Penitentiary. Determination of violent vs. nonviolent crimes other than rape was made upon consultation with a Kentucky Commonwealth Attorney. The results revealed significantly lower IQs for rapists compared to nonrapists convicted of violent crimes and compared to heterogeneous nonrapist convicts. The present research supports the findings of Karacan and co-workers, indicating a general tendency for rapists to have lower IQs than other convicted prisoners.", "PMID": 952615}
{"id": "pubmed23n0032_105", "title": "A current appraisal of side effects of oral contraceptives.", "content": "Oral contraception, although offering almost 100 per cent protection against pregnancy, is associated with many side effects which have resulted in much unnecessary adverse publicity. Such side effects, the majority of which are of annoying nature only, are due to the synthetic hormones used, and have resulted in many changes in formulation and in the withdrawal of several preparations. The types of preparations available are discussed and the side effects appraised from both the patient's and the physician's viewpoint.", "contents": "A current appraisal of side effects of oral contraceptives. Oral contraception, although offering almost 100 per cent protection against pregnancy, is associated with many side effects which have resulted in much unnecessary adverse publicity. Such side effects, the majority of which are of annoying nature only, are due to the synthetic hormones used, and have resulted in many changes in formulation and in the withdrawal of several preparations. The types of preparations available are discussed and the side effects appraised from both the patient's and the physician's viewpoint.", "PMID": 952628}
{"id": "pubmed23n0032_106", "title": "Diabetes in children.", "content": "Juvenile diabetics comprise approximately five per cent of the total diabetic population. The sex incidence in children is approximately equal and it can occur at any age. However, it would seem that the peak ages for its onset in childhood are between eight and twelve years.", "contents": "Diabetes in children. Juvenile diabetics comprise approximately five per cent of the total diabetic population. The sex incidence in children is approximately equal and it can occur at any age. However, it would seem that the peak ages for its onset in childhood are between eight and twelve years.", "PMID": 952630}
{"id": "pubmed23n0032_107", "title": "The need for psychosexual counselling in family planning practice.", "content": "In this review article, the case in favour of the need for psychosexual counselling in family planning practice will be presented. Since psychosexual counselling is a multidisciplinary subject, family planning clinics and practices are ideally suited to the task. In presenting the case, liberal illustrative examples are provided in order to depict what the current and suggested extended facilities and techniques involve. The article is also intended to act as a comprehensive guide for students, nurses, medical practitioners and specialists alike, to bring them up to date in new concepts in history taking, physical examination and the sexually explicit aspects of family planning. Traditionally taught concepts may well be out of date in a modern society.", "contents": "The need for psychosexual counselling in family planning practice. In this review article, the case in favour of the need for psychosexual counselling in family planning practice will be presented. Since psychosexual counselling is a multidisciplinary subject, family planning clinics and practices are ideally suited to the task. In presenting the case, liberal illustrative examples are provided in order to depict what the current and suggested extended facilities and techniques involve. The article is also intended to act as a comprehensive guide for students, nurses, medical practitioners and specialists alike, to bring them up to date in new concepts in history taking, physical examination and the sexually explicit aspects of family planning. Traditionally taught concepts may well be out of date in a modern society.", "PMID": 952631}
{"id": "pubmed23n0032_108", "title": "Diabetes mellitus. A report from the Australian General Practitioner Morbidity and Prescribing Survey 1969-1974.", "content": "The Royal Australian College of General Practitioners, in association with Intercontinental Medical Statistics conducted a General Practice Morbidity and Prescribing Survey from 1.4.69 to 31.12.74. This gave a baseline study of morbidity as it occurs in Australia plus a breakdown by State, sex, age and season, to give a clearer idea of geographical, personal and epidemiological factors influencing health and sickness.", "contents": "Diabetes mellitus. A report from the Australian General Practitioner Morbidity and Prescribing Survey 1969-1974. The Royal Australian College of General Practitioners, in association with Intercontinental Medical Statistics conducted a General Practice Morbidity and Prescribing Survey from 1.4.69 to 31.12.74. This gave a baseline study of morbidity as it occurs in Australia plus a breakdown by State, sex, age and season, to give a clearer idea of geographical, personal and epidemiological factors influencing health and sickness.", "PMID": 952633}
{"id": "pubmed23n0032_109", "title": "The nature and use of purified insulins.", "content": "The limited availability of highly purified insulin preparations for the treatment of diabetes mellitus in australia since 1975, has prompted a review of their history, clinical use and potential in the treatment of diabetics receiving insulin.", "contents": "The nature and use of purified insulins. The limited availability of highly purified insulin preparations for the treatment of diabetes mellitus in australia since 1975, has prompted a review of their history, clinical use and potential in the treatment of diabetics receiving insulin.", "PMID": 952634}
{"id": "pubmed23n0032_110", "title": "Masturbatory guilt and sexual dysfunction.", "content": "Anxiety arising from masturbatory guilt plays a major role in the production of frigidity and impotence. Reduction of this anxiety through greater community awareness of the normality of masturbation is therefore a fertile area for the principles of preventive medicine to be applied.", "contents": "Masturbatory guilt and sexual dysfunction. Anxiety arising from masturbatory guilt plays a major role in the production of frigidity and impotence. Reduction of this anxiety through greater community awareness of the normality of masturbation is therefore a fertile area for the principles of preventive medicine to be applied.", "PMID": 952635}
{"id": "pubmed23n0032_111", "title": "Women and the doctor-patient relationship.", "content": "What little is known about the doctor-patient relationship in Australia has been sought and written from the profession's point of view. This article will concentrate on the consumer; especially women, who have special problems and difficulties not shared with male patients.", "contents": "Women and the doctor-patient relationship. What little is known about the doctor-patient relationship in Australia has been sought and written from the profession's point of view. This article will concentrate on the consumer; especially women, who have special problems and difficulties not shared with male patients.", "PMID": 952636}
{"id": "pubmed23n0032_112", "title": "The care of diabetes mellitus at a community health centre.", "content": "Diabetes mellitus is a chronic disease with a high prevalence of about 2.0 per cent in the australian community. Although hospital diabetic clinics play an important role in the management of diabetic patients, the majority are managed by their local doctor. In this article the possible role of a health centre in the care of diabetics is outlined.", "contents": "The care of diabetes mellitus at a community health centre. Diabetes mellitus is a chronic disease with a high prevalence of about 2.0 per cent in the australian community. Although hospital diabetic clinics play an important role in the management of diabetic patients, the majority are managed by their local doctor. In this article the possible role of a health centre in the care of diabetics is outlined.", "PMID": 952637}
{"id": "pubmed23n0032_113", "title": "A screening test for thyroid function.", "content": "The Achilles tendon reflex half relaxation time measurement (ART) has been used by many physicians both as a diagnostic test and for the assessment of progress in thyroid gland malfunction. Reference is made to some results obtained in Melbourne and in other countries using different methods of measurement of the ART for these purposes. In a series of 2064 patients referred to the Shepherd Foundation Centre, the Achilles tendon reflex half relaxation time was measured by means of the SMI Reflexometer and a comparison was made in each case with a laboratory estimation of the T3 resin uptake and T4 total thyroxine iodine and the Free Thyroxine Index (FTI). Reference is made to a survey conducted among referring doctors where opinions were sought as to the clinical usefulness of different tests including the Achilles tendon reflex time measurement.", "contents": "A screening test for thyroid function. The Achilles tendon reflex half relaxation time measurement (ART) has been used by many physicians both as a diagnostic test and for the assessment of progress in thyroid gland malfunction. Reference is made to some results obtained in Melbourne and in other countries using different methods of measurement of the ART for these purposes. In a series of 2064 patients referred to the Shepherd Foundation Centre, the Achilles tendon reflex half relaxation time was measured by means of the SMI Reflexometer and a comparison was made in each case with a laboratory estimation of the T3 resin uptake and T4 total thyroxine iodine and the Free Thyroxine Index (FTI). Reference is made to a survey conducted among referring doctors where opinions were sought as to the clinical usefulness of different tests including the Achilles tendon reflex time measurement.", "PMID": 952638}
{"id": "pubmed23n0032_114", "title": "Cold adaptation and the anaesthetic requirement for cyclopropane in rats.", "content": "The effect of cold adaptation at 4 degrees C on cyclopropane MAC was studied in 10 Wistar rats over a 16-week period. Compared with a similar control group (normally adapted), there was a significant decrease in weight gain and a significant increase in anaesthetic requirement in the cold-adapted rats.", "contents": "Cold adaptation and the anaesthetic requirement for cyclopropane in rats. The effect of cold adaptation at 4 degrees C on cyclopropane MAC was studied in 10 Wistar rats over a 16-week period. Compared with a similar control group (normally adapted), there was a significant decrease in weight gain and a significant increase in anaesthetic requirement in the cold-adapted rats.", "PMID": 952684}
{"id": "pubmed23n0032_115", "title": "Suxamethonium-induced porcine malignant hyperthermia.", "content": "Metabolic, haemodynamic and neuroendocrine responses to suxamethonium (SCh) were measured in five normal swine and five swine susceptible to malignant hyperthermia (MH), to compare the responses with those previously reported for halothane. Following SCh, the onset of MH was sooner and more abrupt than following halothane. The maximal changes in aerobic metabolism and body temperature sere similar, while the changes in lactate, potassium, hydrogen ion and catecholamine concentrations were smaller than those observed following halothane. These results are discussed in terms of the action of chemical depolarizing drugs such as suxamethonium and acetylcholine. The propagated muscle action potentials produce an increase in the free intracellular calcium concentration which may be self-regenerative, but which may become uncontrollable because of the peculiarities of MH that effect the calcium pump or storage areas.", "contents": "Suxamethonium-induced porcine malignant hyperthermia. Metabolic, haemodynamic and neuroendocrine responses to suxamethonium (SCh) were measured in five normal swine and five swine susceptible to malignant hyperthermia (MH), to compare the responses with those previously reported for halothane. Following SCh, the onset of MH was sooner and more abrupt than following halothane. The maximal changes in aerobic metabolism and body temperature sere similar, while the changes in lactate, potassium, hydrogen ion and catecholamine concentrations were smaller than those observed following halothane. These results are discussed in terms of the action of chemical depolarizing drugs such as suxamethonium and acetylcholine. The propagated muscle action potentials produce an increase in the free intracellular calcium concentration which may be self-regenerative, but which may become uncontrollable because of the peculiarities of MH that effect the calcium pump or storage areas.", "PMID": 952685}
{"id": "pubmed23n0032_116", "title": "Halothane 0.2-4% analysis by gas chromatography.", "content": "The katharometer detector is better suited to the analysis of gaseous halothane (0.2-4%) than is the flame ionization detector, since the peak heights are directly proportional to concentration, and are more reproducible.", "contents": "Halothane 0.2-4% analysis by gas chromatography. The katharometer detector is better suited to the analysis of gaseous halothane (0.2-4%) than is the flame ionization detector, since the peak heights are directly proportional to concentration, and are more reproducible.", "PMID": 952686}
{"id": "pubmed23n0032_117", "title": "Radip induction of ether anaesthesia and controlled maintenacne by a combination of intravenous and inhalation administration without the risk of explosion.", "content": "The induction and maintenance of anaesthesia with ether using a combined intravenous infusion and a constant low inspired concentration are discribed. Predictions from a mathematical model were checked against animal experiments. Anaesthesia occurred within 5 min. The mehtod obviates the need for explosive mixtures.", "contents": "Radip induction of ether anaesthesia and controlled maintenacne by a combination of intravenous and inhalation administration without the risk of explosion. The induction and maintenance of anaesthesia with ether using a combined intravenous infusion and a constant low inspired concentration are discribed. Predictions from a mathematical model were checked against animal experiments. Anaesthesia occurred within 5 min. The mehtod obviates the need for explosive mixtures.", "PMID": 952687}
{"id": "pubmed23n0032_118", "title": "Sequential changes in the fade of tetanic tension after the administration of tubocurarine in anaesthetized man.", "content": "Tetanic and single twitch contractions of the adductor pollicis muscle in anaesthetized man were measured continuously following the i.v. injection of tubocurarine 0.2 mg/kg. Tetanic contractions were depressed earlier by tubocurarine and to a greater extent compared with the single twitch. Recovery was also slower for the tetanic contractions. The fade of tetanic tension was also measured throughout the blockade produced by tubocurarine. Fade was expressed in terms of the tetanic tension ratio: the ratio of the magnitude of the muscle tension at the end of 1 s of tetanic contraction compared with its peak height at the beginning of that contraction. Tetanic tension was well maintained during the onset of the neuromuscular block but was less well maintained during the recovery phase. It is suggested that the assessment of tetanic tension fade shoudl be carried out throughout the various stages of curarization.", "contents": "Sequential changes in the fade of tetanic tension after the administration of tubocurarine in anaesthetized man. Tetanic and single twitch contractions of the adductor pollicis muscle in anaesthetized man were measured continuously following the i.v. injection of tubocurarine 0.2 mg/kg. Tetanic contractions were depressed earlier by tubocurarine and to a greater extent compared with the single twitch. Recovery was also slower for the tetanic contractions. The fade of tetanic tension was also measured throughout the blockade produced by tubocurarine. Fade was expressed in terms of the tetanic tension ratio: the ratio of the magnitude of the muscle tension at the end of 1 s of tetanic contraction compared with its peak height at the beginning of that contraction. Tetanic tension was well maintained during the onset of the neuromuscular block but was less well maintained during the recovery phase. It is suggested that the assessment of tetanic tension fade shoudl be carried out throughout the various stages of curarization.", "PMID": 952688}
{"id": "pubmed23n0032_119", "title": "Effects of Althesin on cerebral blood flow and oxygen consumption in man.", "content": "Cerebral circulation and metabolism during Althesin anaesthesia were studied in seven healthy patients. Althesin was given in a single dose of 0.1 ml/kg and thereafter infused at a constant rate of 0.3 ml/kg/h. During Althesin infusion, the cerebral blood flow (CBF), the cerebral metabolic rate for oxygen (CMRo2) were 29 +/- 10 ml/100 g/min and 1.7 +/- 0.4 ml/133 g/min, respectively. These values were significantly different from those obtained in awake subjects in our laboratory (CBF: 46 +/- 7 ml/100 g/min; CMRo2: 3.1 +/- 0.6 ml/100 g/min). During CBF measurement, the mean cerebral perfusion pressure, cerebral vascular resistance (CVR) and arterial carbon dioxide tension (PaCO2) were 89 +/- 16 mm Hg, 3.4 +/- 1.3 mm Hg/ml/100 g/min, and 36 +/- 9 mm Hg, respectively. The relationship between CBF and PaCO2 were studied and it was found that during Althesin anaesthesia reactivity of cerebral vessels to the alteration of PaCO2 was maintained. It is concluded that Althesin caused cerebral metabolic depression which was accompaned by a decrease in CBF and an increase in CVR.", "contents": "Effects of Althesin on cerebral blood flow and oxygen consumption in man. Cerebral circulation and metabolism during Althesin anaesthesia were studied in seven healthy patients. Althesin was given in a single dose of 0.1 ml/kg and thereafter infused at a constant rate of 0.3 ml/kg/h. During Althesin infusion, the cerebral blood flow (CBF), the cerebral metabolic rate for oxygen (CMRo2) were 29 +/- 10 ml/100 g/min and 1.7 +/- 0.4 ml/133 g/min, respectively. These values were significantly different from those obtained in awake subjects in our laboratory (CBF: 46 +/- 7 ml/100 g/min; CMRo2: 3.1 +/- 0.6 ml/100 g/min). During CBF measurement, the mean cerebral perfusion pressure, cerebral vascular resistance (CVR) and arterial carbon dioxide tension (PaCO2) were 89 +/- 16 mm Hg, 3.4 +/- 1.3 mm Hg/ml/100 g/min, and 36 +/- 9 mm Hg, respectively. The relationship between CBF and PaCO2 were studied and it was found that during Althesin anaesthesia reactivity of cerebral vessels to the alteration of PaCO2 was maintained. It is concluded that Althesin caused cerebral metabolic depression which was accompaned by a decrease in CBF and an increase in CVR.", "PMID": 952689}
{"id": "pubmed23n0032_120", "title": "Suxamethonium-induced hyperkalaemia in patients with severe intra-abdominal infections.", "content": "In nine patients, undergoing repeat operations because of severe intra-abdominal infection developing after major abdominal surgery, serum potassium concentrations were monitored during induction of anaesthesia. Four patients showed an increase of serum potassium ranging from 2.5 to 3.1 mmol/litre above baseline values within 3-6 min after suxamethonium 100 mg i.v. In five patients there was no change. The four patients demonstrating an increase had suffered from pyrexia and leucocytosis for at least 2 weeks. The other five had signs of infection for no more than 9 days. It is concluded that patients with signs of severe intra-abdominal infection lasting longer than 1 week represent an additional category susceptible to suxamethonium-induced hyperkalaemia. They should receive only non-depolarizing muscle relaxants. When the use of suxamethonium is unavoidable, the injection of a non-depolarizing muscle relaxant before the administration of suxamethonium is recommended.", "contents": "Suxamethonium-induced hyperkalaemia in patients with severe intra-abdominal infections. In nine patients, undergoing repeat operations because of severe intra-abdominal infection developing after major abdominal surgery, serum potassium concentrations were monitored during induction of anaesthesia. Four patients showed an increase of serum potassium ranging from 2.5 to 3.1 mmol/litre above baseline values within 3-6 min after suxamethonium 100 mg i.v. In five patients there was no change. The four patients demonstrating an increase had suffered from pyrexia and leucocytosis for at least 2 weeks. The other five had signs of infection for no more than 9 days. It is concluded that patients with signs of severe intra-abdominal infection lasting longer than 1 week represent an additional category susceptible to suxamethonium-induced hyperkalaemia. They should receive only non-depolarizing muscle relaxants. When the use of suxamethonium is unavoidable, the injection of a non-depolarizing muscle relaxant before the administration of suxamethonium is recommended.", "PMID": 952690}
{"id": "pubmed23n0032_121", "title": "Pethidine compared with pethidine-naloxone administered during labour. A study of analgesic treatment by a sequential method.", "content": "In a restricted sequential trial in healthy parturient women, the pain relief from pethidine 100 mg and pethidine 100 mg combined with naloxone 0.4 mg was compared. Pethidine alone gave better relief. The incidence of minor side-effects was high with both treatments but dizziness was reduced slightly by naloxone. It was concluded that naloxone antagonized the analgesia without abolishing the side-effects of pethidine.", "contents": "Pethidine compared with pethidine-naloxone administered during labour. A study of analgesic treatment by a sequential method. In a restricted sequential trial in healthy parturient women, the pain relief from pethidine 100 mg and pethidine 100 mg combined with naloxone 0.4 mg was compared. Pethidine alone gave better relief. The incidence of minor side-effects was high with both treatments but dizziness was reduced slightly by naloxone. It was concluded that naloxone antagonized the analgesia without abolishing the side-effects of pethidine.", "PMID": 952691}
{"id": "pubmed23n0032_122", "title": "Ergometrine, oxytocin and extradural analgesia.", "content": "Blood loss and the incidence of emetic sequelae were assessed in 148 patients undergoing midcavity forceps delivery under continuous lumbar extradural analgesia. Five units of oxytocin i.v. was found to be as effective as ergometrine 0.5 mg i.v. in reducing blood loss at delivery. Nausea, retching or vomiting occurred in 35 (46%) of the mothers who received ergometrine and in none of those who received i.v. oxytocin. The cardiovascular side-effects of ergometrine and oxytocin are reviewed and compared with special reference to patients with hypertension and heart disease. It is suggested that 5 units of oxytocin i.v. should be preferred in these high-risk patients. Because of the absence of an emetic action, i.v. oxytocin is preferable to i.v. ergometrine for patients receiving extradural analgesia.", "contents": "Ergometrine, oxytocin and extradural analgesia. Blood loss and the incidence of emetic sequelae were assessed in 148 patients undergoing midcavity forceps delivery under continuous lumbar extradural analgesia. Five units of oxytocin i.v. was found to be as effective as ergometrine 0.5 mg i.v. in reducing blood loss at delivery. Nausea, retching or vomiting occurred in 35 (46%) of the mothers who received ergometrine and in none of those who received i.v. oxytocin. The cardiovascular side-effects of ergometrine and oxytocin are reviewed and compared with special reference to patients with hypertension and heart disease. It is suggested that 5 units of oxytocin i.v. should be preferred in these high-risk patients. Because of the absence of an emetic action, i.v. oxytocin is preferable to i.v. ergometrine for patients receiving extradural analgesia.", "PMID": 952692}
{"id": "pubmed23n0032_123", "title": "Effects of extradural block: comparison of the properties, circulatory effects and pharmacokinetics of etidocaine and bupivacaine.", "content": "Five healthy, unmedicated male volunteers, aged 19-25 yr, participated in a double-blind, crossover study. Each subject received, on separate occasions and via a catheter placed at L2, 1.5% etiodocaine HCl20 ml with adrenaline 5 mug/ml, or 0.75% bupivacaine HCl 20 ml with adrenaline 5 mug/ml for extradural analgesia. In addition, in order to calculate the absorption rate of the local anaesthetic agent, each subject received on two further occasions etidocaine HCl 75 mg and bupivacaine HCl 75 mg respectively by i.v. infusion, over a period of 10 min. Spread of sensory analgesia to four segments above and below the site of injection was faster with etidocaine (13 +/- 3 min) (mean +/- SD) than with bupivacaine (22 +/- 8 min). Two-segment regression occurred later for bupivacaine (260 +/- 57 min) than for etidocaine (180 +/- 96 min). Caudal spread of analgesia was more extensive with etidocaine than with bupivacaine. The onset of motor blockade tended to be faster with etidocaine (5.8 +/- 3.0 min), than with bupivacaine (10.0 +/- 3.5 min); regression of motor blockade by one unit was longer with etidocaine (306 +/- 103 min) than bupivacaine (238 +/- 75 min). Sudomotor block occurred earlier with etidocaine (4.0 +/- 2.1 min) than bupivacaine (13.7 +/- 4.8 min). Significant changes in cardiac stroke work and stroke volume occurred. For etidocaine these measurements remained below control values for 120-210 min after injection. The mean maximum arterial plasma concentration of etidocaine was 1.52 +/- 0.64 mug/ml, at 14 +/- 2 min and of bupivacaine was 1.35 +/- 0.63 mug/ml, achieved at 20 +/- 4 min. The systemic absorption of both drugs occurred in a biphasic pattern with a fast and slow half-life of 0.3 and approximately 8 h respectively.", "contents": "Effects of extradural block: comparison of the properties, circulatory effects and pharmacokinetics of etidocaine and bupivacaine. Five healthy, unmedicated male volunteers, aged 19-25 yr, participated in a double-blind, crossover study. Each subject received, on separate occasions and via a catheter placed at L2, 1.5% etiodocaine HCl20 ml with adrenaline 5 mug/ml, or 0.75% bupivacaine HCl 20 ml with adrenaline 5 mug/ml for extradural analgesia. In addition, in order to calculate the absorption rate of the local anaesthetic agent, each subject received on two further occasions etidocaine HCl 75 mg and bupivacaine HCl 75 mg respectively by i.v. infusion, over a period of 10 min. Spread of sensory analgesia to four segments above and below the site of injection was faster with etidocaine (13 +/- 3 min) (mean +/- SD) than with bupivacaine (22 +/- 8 min). Two-segment regression occurred later for bupivacaine (260 +/- 57 min) than for etidocaine (180 +/- 96 min). Caudal spread of analgesia was more extensive with etidocaine than with bupivacaine. The onset of motor blockade tended to be faster with etidocaine (5.8 +/- 3.0 min), than with bupivacaine (10.0 +/- 3.5 min); regression of motor blockade by one unit was longer with etidocaine (306 +/- 103 min) than bupivacaine (238 +/- 75 min). Sudomotor block occurred earlier with etidocaine (4.0 +/- 2.1 min) than bupivacaine (13.7 +/- 4.8 min). Significant changes in cardiac stroke work and stroke volume occurred. For etidocaine these measurements remained below control values for 120-210 min after injection. The mean maximum arterial plasma concentration of etidocaine was 1.52 +/- 0.64 mug/ml, at 14 +/- 2 min and of bupivacaine was 1.35 +/- 0.63 mug/ml, achieved at 20 +/- 4 min. The systemic absorption of both drugs occurred in a biphasic pattern with a fast and slow half-life of 0.3 and approximately 8 h respectively.", "PMID": 952693}
{"id": "pubmed23n0032_124", "title": "Influence of anaesthetic technique on postoperative pain. A comparison of anaesthetic supplementation with halothane and with phenoperidine.", "content": "Fifty male patients undergoing elective surgery for duodenal ulcer received either phenoperidine or halothane 0.5% for the supplementation of anaesthesia. The patients in the phenoperidine group required the first postoperative dose of analgesic later and had lower pain scores in the first 2 h after operation. In the course of the 1st, 2nd and 3rd days, the two groups of patients showed a similar pattern after operation with regard to pain scores, vital capacity impairment and oxygen tension measurements.", "contents": "Influence of anaesthetic technique on postoperative pain. A comparison of anaesthetic supplementation with halothane and with phenoperidine. Fifty male patients undergoing elective surgery for duodenal ulcer received either phenoperidine or halothane 0.5% for the supplementation of anaesthesia. The patients in the phenoperidine group required the first postoperative dose of analgesic later and had lower pain scores in the first 2 h after operation. In the course of the 1st, 2nd and 3rd days, the two groups of patients showed a similar pattern after operation with regard to pain scores, vital capacity impairment and oxygen tension measurements.", "PMID": 952694}
{"id": "pubmed23n0032_125", "title": "Personality assessment and postoperative analgesia. A study in male patients undergoing elective gastric surgery.", "content": "In 40 patients undergoing surgery for duodenal ulcer, personality was assessed by a PEN Inventory. After operation, the respiratory restoration factor (RRF) was used to indicate the pain relief from nitrous oxide (25%) and i.v. methadone (three doses of 2 mg). There was no correlation between the psychological factors and RRF. Twenty-five per cent nitrous oxide appeared to be equivalent to i.v. methadone 4 mg in terms of RRF. The degree of hypoxia after surgery correlated with vital capacity impairment and the improvement of oxygen tension after analgesia correlated with RRF after methadone 6 mg.", "contents": "Personality assessment and postoperative analgesia. A study in male patients undergoing elective gastric surgery. In 40 patients undergoing surgery for duodenal ulcer, personality was assessed by a PEN Inventory. After operation, the respiratory restoration factor (RRF) was used to indicate the pain relief from nitrous oxide (25%) and i.v. methadone (three doses of 2 mg). There was no correlation between the psychological factors and RRF. Twenty-five per cent nitrous oxide appeared to be equivalent to i.v. methadone 4 mg in terms of RRF. The degree of hypoxia after surgery correlated with vital capacity impairment and the improvement of oxygen tension after analgesia correlated with RRF after methadone 6 mg.", "PMID": 952695}
{"id": "pubmed23n0032_126", "title": "A useful tube for anaesthesia during laryngeal microsurgery.", "content": "An endotracheal tube for use during anaesthesia for microsurgery of the larynx was made from a plastic endotracheal tube and an angiography connecting tube. It cannot be occluded, requires a minimum of space, and allows jet ventilation of the anaesthetized and paralysed patient. The value of a nitrous oxide-oxygen mixing valve in association with the tube is described.", "contents": "A useful tube for anaesthesia during laryngeal microsurgery. An endotracheal tube for use during anaesthesia for microsurgery of the larynx was made from a plastic endotracheal tube and an angiography connecting tube. It cannot be occluded, requires a minimum of space, and allows jet ventilation of the anaesthetized and paralysed patient. The value of a nitrous oxide-oxygen mixing valve in association with the tube is described.", "PMID": 952696}
{"id": "pubmed23n0032_127", "title": "Mechanisms of leucocyte migration inhibition by breast tumour cell fractions.", "content": "Leucocyte migration inhibition by autologous breast tumour cell fractions was mediated by a soluble factor synthesized and released by mononuclear leucocytes and active against migrating granulocytes. This mechanism is similar to that previously described in respect to cell-mediated sensitivity to microbial antigens. Alternative mechanisms involving directly reactive granulocytes or cytophilic antibodies were rarely operative in the migration tests.", "contents": "Mechanisms of leucocyte migration inhibition by breast tumour cell fractions. Leucocyte migration inhibition by autologous breast tumour cell fractions was mediated by a soluble factor synthesized and released by mononuclear leucocytes and active against migrating granulocytes. This mechanism is similar to that previously described in respect to cell-mediated sensitivity to microbial antigens. Alternative mechanisms involving directly reactive granulocytes or cytophilic antibodies were rarely operative in the migration tests.", "PMID": 952712}
{"id": "pubmed23n0032_128", "title": "Monocytosis associated with the growth of transplanted syngeneic rat sarcomata differing in immunogenicity.", "content": "The effect of the growth of two syngeneic transplanted sarcomata of widely differing biological properties on the number of monocytes in the blood of rats was measured (1) by binding of a specific antimacrophage serum to leucocytes, and (2) by sedimenting in a density gradient rosettes between mononuclear cells and antibody-coated sheep red cells under conditions in which B-cells are not brought down. For the 4 syngeneic sarcomata studied there was a progressive increase in the number of monocytes with tumour growth and the values returned to normal a few days after their surgical removal. The extent of monocytosis was related to the immunogenicity of the tumour and was most pronounced for the HSBPA sarcoma, which is highly immunogenic, has a low rate of spontaneous metastasis and contains many macrophages, and least for the MC-3 sarcoma which is essentially non-immunogenic, invariably gives rise to distant metastases and contains only about 8% macrophages. The growth of sarcomata had previously been found to reduce the number of monocytes which enter inflammatory lesions, both non-specific and due to a delayed hypersensitivity reaction. This \"anti-inflammatory\" action of sarcomata which is related to their immunogenicity cannot be ascribed to the preferential uptake of monocytes by the tumours and it is concluded that the monocytes in the blood of tumour-bearers, though increased in number, are modified so that they do not enter sites of inflammation.", "contents": "Monocytosis associated with the growth of transplanted syngeneic rat sarcomata differing in immunogenicity. The effect of the growth of two syngeneic transplanted sarcomata of widely differing biological properties on the number of monocytes in the blood of rats was measured (1) by binding of a specific antimacrophage serum to leucocytes, and (2) by sedimenting in a density gradient rosettes between mononuclear cells and antibody-coated sheep red cells under conditions in which B-cells are not brought down. For the 4 syngeneic sarcomata studied there was a progressive increase in the number of monocytes with tumour growth and the values returned to normal a few days after their surgical removal. The extent of monocytosis was related to the immunogenicity of the tumour and was most pronounced for the HSBPA sarcoma, which is highly immunogenic, has a low rate of spontaneous metastasis and contains many macrophages, and least for the MC-3 sarcoma which is essentially non-immunogenic, invariably gives rise to distant metastases and contains only about 8% macrophages. The growth of sarcomata had previously been found to reduce the number of monocytes which enter inflammatory lesions, both non-specific and due to a delayed hypersensitivity reaction. This \"anti-inflammatory\" action of sarcomata which is related to their immunogenicity cannot be ascribed to the preferential uptake of monocytes by the tumours and it is concluded that the monocytes in the blood of tumour-bearers, though increased in number, are modified so that they do not enter sites of inflammation.", "PMID": 952713}
{"id": "pubmed23n0032_129", "title": "Stem cell characterization of neutropenia: velocity sedimentation and mass culture analysis.", "content": "Human bone marrow obtained from patients with neutropenia contains a cell population which is absent or diminished in normal marrow. The abnormal population is composed of cells of volume 200-300 mum3 which sediment at 5-5 to 8-5 mm/h. Normal marrow contains one cell class giving rise to increased numbers of grnaulocyte colonies after mass culture, while marrow obtained from neutropenic patients, or from patients with marrow regeneration, shows two such populations; one of these cell classes corresponds to the abnormally large cells demonstrated on velocity sedimentation analysis. This population of large cells may represent a group of either self-renewing cells related to the committed granulocyte progenitors or the pluripotent stem cell.", "contents": "Stem cell characterization of neutropenia: velocity sedimentation and mass culture analysis. Human bone marrow obtained from patients with neutropenia contains a cell population which is absent or diminished in normal marrow. The abnormal population is composed of cells of volume 200-300 mum3 which sediment at 5-5 to 8-5 mm/h. Normal marrow contains one cell class giving rise to increased numbers of grnaulocyte colonies after mass culture, while marrow obtained from neutropenic patients, or from patients with marrow regeneration, shows two such populations; one of these cell classes corresponds to the abnormally large cells demonstrated on velocity sedimentation analysis. This population of large cells may represent a group of either self-renewing cells related to the committed granulocyte progenitors or the pluripotent stem cell.", "PMID": 952714}
{"id": "pubmed23n0032_130", "title": "Rubidazone vs adriamycin: an evaluation of their differential toxicity in the spleen colony assay system.", "content": "Rubidazone, the new semi-synthetic benzol hydrazone hydrochloride derivative of dauorubicin, has proved on a molecular weight basis to be less toxic than adriamycin and similar to daunorubicin in cardiac toxicity studies in the hamster as well as in other in vivo and in vitro test systems. It has proven effectiveness against several animal tumours and human acute leukaemias. We have compared the inhibitory effect of rubidazone to that of adriamycin on P388 leukaemia and normal bone marrow colony-forming units (CFU) using the spleen colony assay system in male DBA2 mice. The efficacy ratios (i.e., the ratio of the slopes of the normal bone marrow CFU to leukaemic CFU dose-survival curves) in the spleen colony assay system for rubidazone and adriamycin were 7-8 and 7-5 respectively. This near identity of efficacy ratios fro rubidazone and adriamycin correlated with the results of median survival time studies in the leukaemic mice. Their dose-median survival time curves were almost parallel, having nearly identical slopes. Rubidazone's equal therapeutic index as compared to adriamycin in the spleen colony assay system together with its known decreased toxicity to cardiac muscle cells makes it an extremely promising new anthracycline derivative to study in comparison to adriamycin in human malignancies.", "contents": "Rubidazone vs adriamycin: an evaluation of their differential toxicity in the spleen colony assay system. Rubidazone, the new semi-synthetic benzol hydrazone hydrochloride derivative of dauorubicin, has proved on a molecular weight basis to be less toxic than adriamycin and similar to daunorubicin in cardiac toxicity studies in the hamster as well as in other in vivo and in vitro test systems. It has proven effectiveness against several animal tumours and human acute leukaemias. We have compared the inhibitory effect of rubidazone to that of adriamycin on P388 leukaemia and normal bone marrow colony-forming units (CFU) using the spleen colony assay system in male DBA2 mice. The efficacy ratios (i.e., the ratio of the slopes of the normal bone marrow CFU to leukaemic CFU dose-survival curves) in the spleen colony assay system for rubidazone and adriamycin were 7-8 and 7-5 respectively. This near identity of efficacy ratios fro rubidazone and adriamycin correlated with the results of median survival time studies in the leukaemic mice. Their dose-median survival time curves were almost parallel, having nearly identical slopes. Rubidazone's equal therapeutic index as compared to adriamycin in the spleen colony assay system together with its known decreased toxicity to cardiac muscle cells makes it an extremely promising new anthracycline derivative to study in comparison to adriamycin in human malignancies.", "PMID": 952715}
{"id": "pubmed23n0032_131", "title": "Methods of classifying and ascertaining children's tumours.", "content": "Several methods of ascertaining and classifying childhood neoplasms for epidemiological study have been evaluated using material from the University of Manchester Children's Tumour Registry (CTR), which includes data from several sources on children with neoplasms first seen in the period 1954-73 who were under 15 years old and living in the Manchester Regional Hospital Board area at the time. Two systems of classification-the International Classification of Diseases (ICD) and the Morphology Section of the Manual of Tumor Nomenclature and Coding (MOTNAC; Percy, Berg and Thomas, 1968)-were tested. No major problems arose with the Morphology Section of MOTNAC, and we recommend that the revised version of this section, in the proposed \"International Classification of Diseases for Oncology\", should be used in epidemiological reports on children's tumours whenever possible. The ICD discriminates less well between the commoner types of childhood neoplasms, but must be retained as a supplementary classification to facilitate international comparisons. A comparison of the completeness of ascertainment achieved in recent years by each source of data showed that more than 98% of the serious cases (neoplasms that were malignant and/or lay within the craniovertebral canal) could have been identified using a combination of Hospital Activity Analysis (HAA) and cancer registration records, and more than 95% using HAA and death records. But in an analysis of 2 years' HAA returns and 6 years' cancer registrations of serious cases, nearly one quarter of the former and one fifth of the latter were shown to record diagnoses which differed from those finally assigned at the CTR. It is concluded that, in epedimiological studies based on routine records, the diagnoses given should always be checked centrally, by experts, in the light of all the available clinical and pathological material (including histological preparations).", "contents": "Methods of classifying and ascertaining children's tumours. Several methods of ascertaining and classifying childhood neoplasms for epidemiological study have been evaluated using material from the University of Manchester Children's Tumour Registry (CTR), which includes data from several sources on children with neoplasms first seen in the period 1954-73 who were under 15 years old and living in the Manchester Regional Hospital Board area at the time. Two systems of classification-the International Classification of Diseases (ICD) and the Morphology Section of the Manual of Tumor Nomenclature and Coding (MOTNAC; Percy, Berg and Thomas, 1968)-were tested. No major problems arose with the Morphology Section of MOTNAC, and we recommend that the revised version of this section, in the proposed \"International Classification of Diseases for Oncology\", should be used in epidemiological reports on children's tumours whenever possible. The ICD discriminates less well between the commoner types of childhood neoplasms, but must be retained as a supplementary classification to facilitate international comparisons. A comparison of the completeness of ascertainment achieved in recent years by each source of data showed that more than 98% of the serious cases (neoplasms that were malignant and/or lay within the craniovertebral canal) could have been identified using a combination of Hospital Activity Analysis (HAA) and cancer registration records, and more than 95% using HAA and death records. But in an analysis of 2 years' HAA returns and 6 years' cancer registrations of serious cases, nearly one quarter of the former and one fifth of the latter were shown to record diagnoses which differed from those finally assigned at the CTR. It is concluded that, in epedimiological studies based on routine records, the diagnoses given should always be checked centrally, by experts, in the light of all the available clinical and pathological material (including histological preparations).", "PMID": 952716}
{"id": "pubmed23n0032_132", "title": "Geographic correlation between mortality from primary hepatic carcinoma and prevalence of hepatitis B surface antigen in Greece.", "content": "Average annual age-adjusted mortality rates per 100,000 from primary hepatic carcinoma (PHC) among males for 1971-1973 in the urban and rural areas of the 9 geographical regions of Greece were estimated. Hepatitis-B surface antigen (HBsAg) prevalence by region and area was evaluated in a sample of 22,844 Greek Air Force recruits from all parts of the country. Mortality from PHC was found significantly higher in urban areas (28-30 vs. 18-81) whereas prevalence of HBsAg was higher in rural areas (5-3% vs. 3-90%). Nevertheless further statistical analysis showed that there is a strong correlation between HBsAg prevalence and mortality from PHC, which is higher in rural (r = + 0-88) than in urban (+ 0-57) areas. The latter findings indicate that hepatitis B infection and PHC may be causally related.", "contents": "Geographic correlation between mortality from primary hepatic carcinoma and prevalence of hepatitis B surface antigen in Greece. Average annual age-adjusted mortality rates per 100,000 from primary hepatic carcinoma (PHC) among males for 1971-1973 in the urban and rural areas of the 9 geographical regions of Greece were estimated. Hepatitis-B surface antigen (HBsAg) prevalence by region and area was evaluated in a sample of 22,844 Greek Air Force recruits from all parts of the country. Mortality from PHC was found significantly higher in urban areas (28-30 vs. 18-81) whereas prevalence of HBsAg was higher in rural areas (5-3% vs. 3-90%). Nevertheless further statistical analysis showed that there is a strong correlation between HBsAg prevalence and mortality from PHC, which is higher in rural (r = + 0-88) than in urban (+ 0-57) areas. The latter findings indicate that hepatitis B infection and PHC may be causally related.", "PMID": 952717}
{"id": "pubmed23n0032_133", "title": "Selection of skin tests in childhood asthma.", "content": "Skin tests have been applied to 161 asthmatic children seen consecutively at a paediatric respiratory clinic. To demonstrate whether a child's asthma is associated with a positive skin test it is suggested that only four extracts need be applied: house dust, house dust mite (D. farinae), grass pollens and a control.", "contents": "Selection of skin tests in childhood asthma. Skin tests have been applied to 161 asthmatic children seen consecutively at a paediatric respiratory clinic. To demonstrate whether a child's asthma is associated with a positive skin test it is suggested that only four extracts need be applied: house dust, house dust mite (D. farinae), grass pollens and a control.", "PMID": 952720}
{"id": "pubmed23n0032_134", "title": "Thoracoabdominal aortic aneurysm presenting as a solitary pulmonary nodule.", "content": "Bronchogenic carcinoma was suspected in an elderly male smoker who had lost weight and presented with a large solitary pulmonary nodule on the radiograph. On thoracotomy a thoracoabdominal aortic aneurysm was found. The diographic findings in this case and the characteristics of thoracoabdominal aneurysms in general are briefly discussed.", "contents": "Thoracoabdominal aortic aneurysm presenting as a solitary pulmonary nodule. Bronchogenic carcinoma was suspected in an elderly male smoker who had lost weight and presented with a large solitary pulmonary nodule on the radiograph. On thoracotomy a thoracoabdominal aortic aneurysm was found. The diographic findings in this case and the characteristics of thoracoabdominal aneurysms in general are briefly discussed.", "PMID": 952721}
{"id": "pubmed23n0032_135", "title": "Eosinophilic granuloma of the lung.", "content": "Pulmonary eosinophilic granuloma diagnosed by lung biopsy is described; the case demonstrates the remission and relapse that may occur.", "contents": "Eosinophilic granuloma of the lung. Pulmonary eosinophilic granuloma diagnosed by lung biopsy is described; the case demonstrates the remission and relapse that may occur.", "PMID": 952723}
{"id": "pubmed23n0032_136", "title": "Sarcoidosis and thyrotoxicosis: A study of five patients.", "content": "Five patients with sarcoidosis and thyrotoxicosis have been studied. Autoimmunity resulting from loss of thymus-derived lymphocyte control is suggested as a cause common to both.", "contents": "Sarcoidosis and thyrotoxicosis: A study of five patients. Five patients with sarcoidosis and thyrotoxicosis have been studied. Autoimmunity resulting from loss of thymus-derived lymphocyte control is suggested as a cause common to both.", "PMID": 952724}
{"id": "pubmed23n0032_137", "title": "Sleeping ventilatory patterns in patients with severe chronic airflow obstruction causing respiratory failure.", "content": "During sleep some patients with airways obstruction and hypoxaemia developed tachypnoea. This could not be explained by the severity of their abnormality of lung function, their CO2 responsiveness, the nature of their lung disease or their personality. This nocturnal tachypnoea correlated best with a raised resting arterial blood PCO2, and was not seen hypoxaemic patients with a normal PCO2 who showed the usual fall in respiratory rate when asleep. We suggest that in patients with both hypoxaemia and hypercapnia sleep removes a cortical inhibitory mechanism which slows breathing durigng waking hours, and is linked to the arterial blood PCO2.", "contents": "Sleeping ventilatory patterns in patients with severe chronic airflow obstruction causing respiratory failure. During sleep some patients with airways obstruction and hypoxaemia developed tachypnoea. This could not be explained by the severity of their abnormality of lung function, their CO2 responsiveness, the nature of their lung disease or their personality. This nocturnal tachypnoea correlated best with a raised resting arterial blood PCO2, and was not seen hypoxaemic patients with a normal PCO2 who showed the usual fall in respiratory rate when asleep. We suggest that in patients with both hypoxaemia and hypercapnia sleep removes a cortical inhibitory mechanism which slows breathing durigng waking hours, and is linked to the arterial blood PCO2.", "PMID": 952725}
{"id": "pubmed23n0032_138", "title": "The prevalence of asthma and wheezing in children.", "content": "The prevalence of asthma and wheezing in children in Birmingham in 1974-5 was compared with the previous studies in 1968-9 and 1956-7. The frequency of asthma increased over the 20-year period, although the rate of increase had slowed. Asthma was commoner in boys than in girls. Children of the same racial group were more prone to asthma, and probably to other atopic diseases, if born in England than if born in poor tropical countries. Environmental factors in early life profoundly influence the prevalence of asthma in childhood.", "contents": "The prevalence of asthma and wheezing in children. The prevalence of asthma and wheezing in children in Birmingham in 1974-5 was compared with the previous studies in 1968-9 and 1956-7. The frequency of asthma increased over the 20-year period, although the rate of increase had slowed. Asthma was commoner in boys than in girls. Children of the same racial group were more prone to asthma, and probably to other atopic diseases, if born in England than if born in poor tropical countries. Environmental factors in early life profoundly influence the prevalence of asthma in childhood.", "PMID": 952726}
{"id": "pubmed23n0032_139", "title": "Drug-induced proteolysis: a correlation with oedema-reducing ability.", "content": "A very strong correlation has been shown to exist between acid and neutral protease activity levels in the skin, the acid protease activity level of the oedema fluid, and the oedema-reducing ability of the benzo-pyrones and related drugs. Macrophages, which are believed to be the main cells affected by the drugs, are very common in thermally injured tissues. Their lysosomal enzymes work at an acid pH. Since the main acid protease is cathepsin D, the overall acid protease levels are representative of changes in cathepsin D levels. Elevated levels are concomitant with more complete and rapid digestion of accumulated protein. The resulting fragments then can rapidly leave the injured tissues, freeing the oedema fluid. This form of proteolysis is very much different from that which is used by pharmacologists as a measure of inflammation. Normal proteolysis in inflammation represents an estimate of tissue derangement, but the proteolysis induced by drugs such as the benzo-pyrones represents a means of lessening some of the more injurious effects of this derangement. The results presented here strongly confirm this.", "contents": "Drug-induced proteolysis: a correlation with oedema-reducing ability. A very strong correlation has been shown to exist between acid and neutral protease activity levels in the skin, the acid protease activity level of the oedema fluid, and the oedema-reducing ability of the benzo-pyrones and related drugs. Macrophages, which are believed to be the main cells affected by the drugs, are very common in thermally injured tissues. Their lysosomal enzymes work at an acid pH. Since the main acid protease is cathepsin D, the overall acid protease levels are representative of changes in cathepsin D levels. Elevated levels are concomitant with more complete and rapid digestion of accumulated protein. The resulting fragments then can rapidly leave the injured tissues, freeing the oedema fluid. This form of proteolysis is very much different from that which is used by pharmacologists as a measure of inflammation. Normal proteolysis in inflammation represents an estimate of tissue derangement, but the proteolysis induced by drugs such as the benzo-pyrones represents a means of lessening some of the more injurious effects of this derangement. The results presented here strongly confirm this.", "PMID": 952727}
{"id": "pubmed23n0032_140", "title": "Age and sex differences in the response of lymph node post-capillary venules in mice infected with Toxoplasma gondii.", "content": "Groups of male and female mice of varying ages were infected with a low-virulence strain of Toxoplasma gondii and killed 3 weeks and 6 weeks after infection. The lymph nodes of the female group showed a greater prominence of the endothelial cells of the post-capillary venules. This difference was most marked at the age of 15 weeks and least at 30 or more weeks. The same sex difference was demonstrated in control mice although to a lesser extent. The possible role is discussed of the effect of female sex hormones on vascular endothelium in modifying the development of an immune response and in explaining the observed female preponderance in human cases of auto-immune disease.", "contents": "Age and sex differences in the response of lymph node post-capillary venules in mice infected with Toxoplasma gondii. Groups of male and female mice of varying ages were infected with a low-virulence strain of Toxoplasma gondii and killed 3 weeks and 6 weeks after infection. The lymph nodes of the female group showed a greater prominence of the endothelial cells of the post-capillary venules. This difference was most marked at the age of 15 weeks and least at 30 or more weeks. The same sex difference was demonstrated in control mice although to a lesser extent. The possible role is discussed of the effect of female sex hormones on vascular endothelium in modifying the development of an immune response and in explaining the observed female preponderance in human cases of auto-immune disease.", "PMID": 952728}
{"id": "pubmed23n0032_141", "title": "Biochemical effects of asbestiform minerals on lung fibroblast cultures.", "content": "The ability of different types of asbestiform minerals to enhance or suppress the levels of fibrous collagen in cultures of lung fibroblasts was tested. All commercially important asbestos dusts produced both effects, a lower dose favouring a fibrogenic response whereas a higher dose favoured an opposite effect.", "contents": "Biochemical effects of asbestiform minerals on lung fibroblast cultures. The ability of different types of asbestiform minerals to enhance or suppress the levels of fibrous collagen in cultures of lung fibroblasts was tested. All commercially important asbestos dusts produced both effects, a lower dose favouring a fibrogenic response whereas a higher dose favoured an opposite effect.", "PMID": 952729}
{"id": "pubmed23n0032_142", "title": "DNA content of cultured mammalian cells exposed to smoke and smoke fractions from cigarettes containing tobacco or NSM, a tobacco substitute.", "content": "The effects of smoke and smoke fractions from tobacco and a substitute smoking material (NSM) on the DNA content of mammaliam cells in culture were measured. Tobacco smoke caused significant (P less than 0.001) changes in the DNA content of all the mammalian cells exposed compared with controls. NSM smoke did not have a significant effect on the DNA content of the exposed cells (P less than 0.95). Smoke from blends of NSM and tobacco caused changes in DNA content in proportion to the amount of tobacco in the mixtures. Condensate from cigarettes containing tobacco or blends of tobacco and NSM caused significant (P less than 0.001) changes in DNA content of mammalian cell populations in culture, whereas equal weights of condensate from NSM alone or NSM containing nicotine did not cause significant changes (P less than 0.05). NSM produces 28% of the weight of condensate per cigarette in comparison with tabacco and would, therefore, be expected to be far less biologically active than tobacco. Filtered smoke from cigarettes containing tobacco caused significant (P less than 0.001) changes in the DNA content of mammalian cells in culture. These changes were quantitatively similar to those caused by whole smoke suggesting that the gas phase of cigarette smoke is biologically more reactive than the particulate phase. The filtered smoke from the substitute smoking material NSM did not cause significant (P less than 0.95) changes in DNA content of cultured mammalian cells. Filtered smoke from blends of NSM and tobacco caused changes in DNA content in proportion to the amount of tobacco in the mixture.", "contents": "DNA content of cultured mammalian cells exposed to smoke and smoke fractions from cigarettes containing tobacco or NSM, a tobacco substitute. The effects of smoke and smoke fractions from tobacco and a substitute smoking material (NSM) on the DNA content of mammaliam cells in culture were measured. Tobacco smoke caused significant (P less than 0.001) changes in the DNA content of all the mammalian cells exposed compared with controls. NSM smoke did not have a significant effect on the DNA content of the exposed cells (P less than 0.95). Smoke from blends of NSM and tobacco caused changes in DNA content in proportion to the amount of tobacco in the mixtures. Condensate from cigarettes containing tobacco or blends of tobacco and NSM caused significant (P less than 0.001) changes in DNA content of mammalian cell populations in culture, whereas equal weights of condensate from NSM alone or NSM containing nicotine did not cause significant changes (P less than 0.05). NSM produces 28% of the weight of condensate per cigarette in comparison with tabacco and would, therefore, be expected to be far less biologically active than tobacco. Filtered smoke from cigarettes containing tobacco caused significant (P less than 0.001) changes in the DNA content of mammalian cells in culture. These changes were quantitatively similar to those caused by whole smoke suggesting that the gas phase of cigarette smoke is biologically more reactive than the particulate phase. The filtered smoke from the substitute smoking material NSM did not cause significant (P less than 0.95) changes in DNA content of cultured mammalian cells. Filtered smoke from blends of NSM and tobacco caused changes in DNA content in proportion to the amount of tobacco in the mixture.", "PMID": 952730}
{"id": "pubmed23n0032_143", "title": "Pathogenesis and pathology of respiratory tularaemia in the rabbit.", "content": "The development of pathological lesions in the organs of rabbits was examined at intervals from 1 h to 4 days after aerosol infection with Francisella tularensis. The earliest change, accumulation of polymorphonuclear leucocytes (PMN) in ulmonary alveolar ducts, occurred at 19 h. From the 2nd day multiple foci of necrosis and PMN infiltration were present in large airways and alveoli throughout the lungs and progressively increased in size. Pulmonary arteritis was a prominent feature of the infection. Areas of necrosis were present in the nasal mucosa, pharynz and trachea, and pyogranolomatous lesions consistently developed in the liver, spleen, and lymph nodes.", "contents": "Pathogenesis and pathology of respiratory tularaemia in the rabbit. The development of pathological lesions in the organs of rabbits was examined at intervals from 1 h to 4 days after aerosol infection with Francisella tularensis. The earliest change, accumulation of polymorphonuclear leucocytes (PMN) in ulmonary alveolar ducts, occurred at 19 h. From the 2nd day multiple foci of necrosis and PMN infiltration were present in large airways and alveoli throughout the lungs and progressively increased in size. Pulmonary arteritis was a prominent feature of the infection. Areas of necrosis were present in the nasal mucosa, pharynz and trachea, and pyogranolomatous lesions consistently developed in the liver, spleen, and lymph nodes.", "PMID": 952731}
{"id": "pubmed23n0032_144", "title": "Excision repair of DNA in some photodermatoses.", "content": "Excision repair of DNA was measured in a number of photosensitive subjects by estimating unscheduled DNA synthesis in cultured fibroblasts after ultraviolet radiation. The amount of unscheduled DNA synthesis and the action spectrum for this process were estimated in each subject and compared with seven normal subjects. Two subjects with polymorphic light eruption and three with actinic reticuloid exhibited normal levels of DNA repair and normal action spectra. In one subject with classical xeroderma pigmentosum no excision repair could be demonstrated whilst in another the amount of repair was lower than normal. The finding of normal DNA excision repair in polymorphic light eruption does not support an earlier claim that this repair process is abnormal in this common photodermatosis.", "contents": "Excision repair of DNA in some photodermatoses. Excision repair of DNA was measured in a number of photosensitive subjects by estimating unscheduled DNA synthesis in cultured fibroblasts after ultraviolet radiation. The amount of unscheduled DNA synthesis and the action spectrum for this process were estimated in each subject and compared with seven normal subjects. Two subjects with polymorphic light eruption and three with actinic reticuloid exhibited normal levels of DNA repair and normal action spectra. In one subject with classical xeroderma pigmentosum no excision repair could be demonstrated whilst in another the amount of repair was lower than normal. The finding of normal DNA excision repair in polymorphic light eruption does not support an earlier claim that this repair process is abnormal in this common photodermatosis.", "PMID": 952736}
{"id": "pubmed23n0032_145", "title": "A follow-up study of patients with recurrent urticaria and hypersensitivity to aspirin, benzoates and azo dyes.", "content": "We have studied seventy-five patients with recurrent urticaria and angio-oedema of more than 4 months duration and with positive provocation tests to aspirin, azo dyes, and/or benzoates. Cross-reactions between the test compounds were common. The patients were recommended to be on a diet free from salicylates, benzoates, and azo dyes. They were then followed for 6-24 months. At the follow-up, 24% were free from symptoms, 57% considered themselves much better and 19% stated that they were slightly better or unchanged. All patients had followed the diet for at least 1-3 months. Most of those who became totally free of symptoms did not continue with the diet, while most of the patients who considered themselves much better found that it was necessary to continue on the recommended diet. They usually developed symptoms as soon as they ingested something containing azo dyes or benzoates. To be able to maintain such a diet, it is important that the content of additives in food and drugs be properly declared.", "contents": "A follow-up study of patients with recurrent urticaria and hypersensitivity to aspirin, benzoates and azo dyes. We have studied seventy-five patients with recurrent urticaria and angio-oedema of more than 4 months duration and with positive provocation tests to aspirin, azo dyes, and/or benzoates. Cross-reactions between the test compounds were common. The patients were recommended to be on a diet free from salicylates, benzoates, and azo dyes. They were then followed for 6-24 months. At the follow-up, 24% were free from symptoms, 57% considered themselves much better and 19% stated that they were slightly better or unchanged. All patients had followed the diet for at least 1-3 months. Most of those who became totally free of symptoms did not continue with the diet, while most of the patients who considered themselves much better found that it was necessary to continue on the recommended diet. They usually developed symptoms as soon as they ingested something containing azo dyes or benzoates. To be able to maintain such a diet, it is important that the content of additives in food and drugs be properly declared.", "PMID": 952737}
{"id": "pubmed23n0032_146", "title": "Necropsy and ultrastructural findings in histiocytic medullary reticulosis.", "content": "In a case of histiocytic medullary reticulosis with protracted course and necrotic skin lesions, histological study revealed infiltration of the fat tissue by atypical reticulohistiocytic cells, areas of associated fat necrosis and vascular lesions. Autophagocytosis (phagocytosis of erythrocytes, erythroblasts, platelets, lymphoid cells, and nuclear debris) was found in biopsy specimens of the subcutaneous infiltrates, lymph nodes, and bone marrow, and in necropsy specimens of skin, iliac mesenteric lymph nodes, bone marrow, liver, spleen, and suprarenal glands. Electron microscopy of various viscera showed abnormal histiocytic cells containing multiple phagocytosed erythrocytes and other blood cells in different stages of destruction and digestion. Multi-membranous bodies also were present. Defective lysosomal degradation of intracytoplasmatic ceroids in this condition is postulated.", "contents": "Necropsy and ultrastructural findings in histiocytic medullary reticulosis. In a case of histiocytic medullary reticulosis with protracted course and necrotic skin lesions, histological study revealed infiltration of the fat tissue by atypical reticulohistiocytic cells, areas of associated fat necrosis and vascular lesions. Autophagocytosis (phagocytosis of erythrocytes, erythroblasts, platelets, lymphoid cells, and nuclear debris) was found in biopsy specimens of the subcutaneous infiltrates, lymph nodes, and bone marrow, and in necropsy specimens of skin, iliac mesenteric lymph nodes, bone marrow, liver, spleen, and suprarenal glands. Electron microscopy of various viscera showed abnormal histiocytic cells containing multiple phagocytosed erythrocytes and other blood cells in different stages of destruction and digestion. Multi-membranous bodies also were present. Defective lysosomal degradation of intracytoplasmatic ceroids in this condition is postulated.", "PMID": 952738}
{"id": "pubmed23n0032_147", "title": "Hypersensitivity of scleroderma cutaneous vascular smooth muscle to 5-hydroxytryptamine.", "content": "Vascular smooth muscle strips from subcutaneous wrist vessels of patients with scleroderma (systemic sclerosis) were studied in an isolated system. No difference between catecholamine response of scleroderma or control vessel strips was observed. Three of four patients demonstrated significant hypersensitivity to 5-hydroxytryptamine as compared to control values. Serotonin hypersensitivity may play a role in the evolution of Raynaud's phenomenon and vascular scleroderma.", "contents": "Hypersensitivity of scleroderma cutaneous vascular smooth muscle to 5-hydroxytryptamine. Vascular smooth muscle strips from subcutaneous wrist vessels of patients with scleroderma (systemic sclerosis) were studied in an isolated system. No difference between catecholamine response of scleroderma or control vessel strips was observed. Three of four patients demonstrated significant hypersensitivity to 5-hydroxytryptamine as compared to control values. Serotonin hypersensitivity may play a role in the evolution of Raynaud's phenomenon and vascular scleroderma.", "PMID": 952739}
{"id": "pubmed23n0032_148", "title": "The prognosis of systemic sclerosis.", "content": "Eighty-four patients with systemic sclerosis have been investigated and observed at intervals for up to 15 years. The prognosis is worse in males than in females although in any individual case the clinical course is unpredictable. Those patients with the combination of calcinosis, Raynaud's phenomenon, sclerodactyly and telangiectasia have the same degree of systemic involvement and the same prognosis as other patients with systemic sclerosis, and the term 'CRST syndrome' should be abandoned.", "contents": "The prognosis of systemic sclerosis. Eighty-four patients with systemic sclerosis have been investigated and observed at intervals for up to 15 years. The prognosis is worse in males than in females although in any individual case the clinical course is unpredictable. Those patients with the combination of calcinosis, Raynaud's phenomenon, sclerodactyly and telangiectasia have the same degree of systemic involvement and the same prognosis as other patients with systemic sclerosis, and the term 'CRST syndrome' should be abandoned.", "PMID": 952740}
{"id": "pubmed23n0032_149", "title": "An auto-antibody profile in alopecia totalis and diffuse alopecia.", "content": "Thirty-one patients suffering from alopecia totalis and fourteen patients with idiopathic diffuse alopecia were examined for evidence of an endocrinopathy and their sera screened for organ-specific auto-antibodies. The results were compared with matched control groups of dermatological patients and a random group of dermatological patients. There was no statistically significant difference in the groups and the results were similar to those found in the general population.", "contents": "An auto-antibody profile in alopecia totalis and diffuse alopecia. Thirty-one patients suffering from alopecia totalis and fourteen patients with idiopathic diffuse alopecia were examined for evidence of an endocrinopathy and their sera screened for organ-specific auto-antibodies. The results were compared with matched control groups of dermatological patients and a random group of dermatological patients. There was no statistically significant difference in the groups and the results were similar to those found in the general population.", "PMID": 952741}
{"id": "pubmed23n0032_150", "title": "Immunological demonstration of multiple esterases in human eccrine sweat.", "content": "Eccrine sweat collected from the human skin surface contains at least five different esterases. One of them  is a cholinesterase. A non-specific carboxylesterase with the electrophoretic mobility of an alpha-globulin appears to be a serum protein. Besides this, there are two isoenzymes of human origin migrating with the same electrophoretic mobility as gamma-globulins. These two isoenzymes are immunologically identical with a non-specific carboxylesterase occurring in numerous organs and body fluids. Lipase activity could not be demonstrated.", "contents": "Immunological demonstration of multiple esterases in human eccrine sweat. Eccrine sweat collected from the human skin surface contains at least five different esterases. One of them  is a cholinesterase. A non-specific carboxylesterase with the electrophoretic mobility of an alpha-globulin appears to be a serum protein. Besides this, there are two isoenzymes of human origin migrating with the same electrophoretic mobility as gamma-globulins. These two isoenzymes are immunologically identical with a non-specific carboxylesterase occurring in numerous organs and body fluids. Lipase activity could not be demonstrated.", "PMID": 952742}
{"id": "pubmed23n0032_151", "title": "Septrin in the treatment of dermatitis cruris pustulosa et atrophicans.", "content": "The results of treatment of 30 Nigerian patients suffering from dermatitis cruris pustulosa et atrophicans by a 7-week course of Septrin are described. Septrin was effective in twenty-three of twenty-four patients who completed the study. The best therapeutic effects were observed in the early, pustular phase of the disease.", "contents": "Septrin in the treatment of dermatitis cruris pustulosa et atrophicans. The results of treatment of 30 Nigerian patients suffering from dermatitis cruris pustulosa et atrophicans by a 7-week course of Septrin are described. Septrin was effective in twenty-three of twenty-four patients who completed the study. The best therapeutic effects were observed in the early, pustular phase of the disease.", "PMID": 952743}
{"id": "pubmed23n0032_152", "title": "The treatment of psoriatic nail dystrophy with intradermal steroid injections.", "content": "Thirty-seven patients with psoriatic nail dystrophy were treated with intradermal nail-fold injections of either triamcinolone acetonide or triamcinolone hexacetonide, using the Port-O-Jet needleless injector. Mild nail-matrix changes showed a very good response to treatment, whilst nail-bed and/or hyponychial changes causing onycholysis and the more severe types of dystrophy usually showed little improvement. Although in occasional cases this may be a useful form of treatment, we would not recommend its general use in patients with psoriatic nail dystrophy.", "contents": "The treatment of psoriatic nail dystrophy with intradermal steroid injections. Thirty-seven patients with psoriatic nail dystrophy were treated with intradermal nail-fold injections of either triamcinolone acetonide or triamcinolone hexacetonide, using the Port-O-Jet needleless injector. Mild nail-matrix changes showed a very good response to treatment, whilst nail-bed and/or hyponychial changes causing onycholysis and the more severe types of dystrophy usually showed little improvement. Although in occasional cases this may be a useful form of treatment, we would not recommend its general use in patients with psoriatic nail dystrophy.", "PMID": 952744}
{"id": "pubmed23n0032_153", "title": "High dose dapsone toxicity.", "content": "A description of the clinical and biochemical course is given for a patient who developed haemolysis and methaemoglobinaemia following dapsone therapy in high dosage for 2 weeks.", "contents": "High dose dapsone toxicity. A description of the clinical and biochemical course is given for a patient who developed haemolysis and methaemoglobinaemia following dapsone therapy in high dosage for 2 weeks.", "PMID": 952745}
{"id": "pubmed23n0032_154", "title": "Prurigo nodularis and gluten enteropathy.", "content": "Two cases of prurigo nodularis are described. Each is considered to have a gluten sensitive enteropathy. This association may be one facet of prurigo nodularis.", "contents": "Prurigo nodularis and gluten enteropathy. Two cases of prurigo nodularis are described. Each is considered to have a gluten sensitive enteropathy. This association may be one facet of prurigo nodularis.", "PMID": 952746}
{"id": "pubmed23n0032_155", "title": "Carbohydrate metabolism in lichen planus.", "content": "A study of forty patients with active lichen planus and a negative family history for diabetes showed that 42% had unequivocally abnormal oral glucose tolerance. The pattern of insulin response to glucose is similar to that seen in typical mild maturity-onset diabetes. There was no association between the presence of glucose intolerance and the duration or type of lesions. None of the patients with glucose intolerance had demonstrable islet-cell antibodies.", "contents": "Carbohydrate metabolism in lichen planus. A study of forty patients with active lichen planus and a negative family history for diabetes showed that 42% had unequivocally abnormal oral glucose tolerance. The pattern of insulin response to glucose is similar to that seen in typical mild maturity-onset diabetes. There was no association between the presence of glucose intolerance and the duration or type of lesions. None of the patients with glucose intolerance had demonstrable islet-cell antibodies.", "PMID": 952747}
{"id": "pubmed23n0032_156", "title": "A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro.", "content": "Small biopsies of skin, and of keratinized and non-keratinized oral mucosa were incubated with horseradish peroxidase, fixed and reacted to demonstrate peroxidase activity at the light and electron microscope level. In all tissues peroxidase extended through the intercellular spaces of the epithelium up to, but not into, the stratum corneum of keratinized, or the superficial layer of non-keratinized, tissue. This distribution corresponds to that seen in studies using the tracer in vivo where the presence of an intercellular permeability barrier has been demonstrated in the upper cell layers. It is suggested that the method is a useful way of investigating the permeability of normal and pathological human epithelial tissues.", "contents": "A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro. Small biopsies of skin, and of keratinized and non-keratinized oral mucosa were incubated with horseradish peroxidase, fixed and reacted to demonstrate peroxidase activity at the light and electron microscope level. In all tissues peroxidase extended through the intercellular spaces of the epithelium up to, but not into, the stratum corneum of keratinized, or the superficial layer of non-keratinized, tissue. This distribution corresponds to that seen in studies using the tracer in vivo where the presence of an intercellular permeability barrier has been demonstrated in the upper cell layers. It is suggested that the method is a useful way of investigating the permeability of normal and pathological human epithelial tissues.", "PMID": 952748}
{"id": "pubmed23n0032_157", "title": "Sociological implications of an epidemiological study of eczema in the City of Birmingham.", "content": "An epidemiological study has been made of 4696 cases of eczematous dermatitis referred to hospital outpatient clinics in the City of Birmingham over a 3-year period. Some sociological implications of the investigation are discussed. The need for further sociological accounts of skin disease is stressed.", "contents": "Sociological implications of an epidemiological study of eczema in the City of Birmingham. An epidemiological study has been made of 4696 cases of eczematous dermatitis referred to hospital outpatient clinics in the City of Birmingham over a 3-year period. Some sociological implications of the investigation are discussed. The need for further sociological accounts of skin disease is stressed.", "PMID": 952749}
{"id": "pubmed23n0032_158", "title": "Complement component profiles in urticaria, dermatitis herpetiformis, and alopecia areata.", "content": "Insignificant variation was noted in the mean levels of the complement components CIQ, C4, C2, C3, C5 and C3PA between various groups of sera from patients with urticaria, angio-oedema, dermatitis herpetiformis, alopecia areata, or hay fever. No deficiencies were found. Elevated C9 levels in chronic urticaria and angio-oedema reflected its nature as an acute phase protein. The assessment of C components in single or sequential serum samples revealed only marginal variations. Similar results were recorded for plasminogen the immunoglobulins, G, A and M. Some evidence is provided for the primary involvement of the kallikrein-kinin system in urticaria.", "contents": "Complement component profiles in urticaria, dermatitis herpetiformis, and alopecia areata. Insignificant variation was noted in the mean levels of the complement components CIQ, C4, C2, C3, C5 and C3PA between various groups of sera from patients with urticaria, angio-oedema, dermatitis herpetiformis, alopecia areata, or hay fever. No deficiencies were found. Elevated C9 levels in chronic urticaria and angio-oedema reflected its nature as an acute phase protein. The assessment of C components in single or sequential serum samples revealed only marginal variations. Similar results were recorded for plasminogen the immunoglobulins, G, A and M. Some evidence is provided for the primary involvement of the kallikrein-kinin system in urticaria.", "PMID": 952750}
{"id": "pubmed23n0032_159", "title": "Erythropoietic protoporphyria. Hepatic implications.", "content": "The terminal stages of erythropoietic protoporphyria are recorded. The observations are related to the site of the fundamental lesion and the nature of the biochemical defect. The possibly ominous prognosis in this usually mild condition is emphasized. Apart from congenital porphyria, the porphyrias do not usually confer severe cutaneous lesions. These diseases present to dermatologists because of moderate photosensitivity and are not usually regarded as a risk to life. Dangerous central nervous system involvement may occur, however, in acute intermittent, variegate and hereditary coproporphyrias, while in acquired symptomatic porphyria severe underlying liver dysfunction may occur. Probably the most common familial photosensitizing porphyria is erythropoietic protoporphyria. Recently some deaths from severe liver involvement have been reported in this disease.", "contents": "Erythropoietic protoporphyria. Hepatic implications. The terminal stages of erythropoietic protoporphyria are recorded. The observations are related to the site of the fundamental lesion and the nature of the biochemical defect. The possibly ominous prognosis in this usually mild condition is emphasized. Apart from congenital porphyria, the porphyrias do not usually confer severe cutaneous lesions. These diseases present to dermatologists because of moderate photosensitivity and are not usually regarded as a risk to life. Dangerous central nervous system involvement may occur, however, in acute intermittent, variegate and hereditary coproporphyrias, while in acquired symptomatic porphyria severe underlying liver dysfunction may occur. Probably the most common familial photosensitizing porphyria is erythropoietic protoporphyria. Recently some deaths from severe liver involvement have been reported in this disease.", "PMID": 952751}
{"id": "pubmed23n0032_160", "title": "HL-A antigens in patients with guttate psoriasis.", "content": "Tissue typing was performed on lymphocytes of sixty-two patients with guttate psoriasis, in forty-four of whom this was the first manifestations of the disease. In 84% of cases, the guttate psoriasis was preceded by a clinical infection. A highly significant excess of antigen W17 (HLA-BW17) was found in patients when compared with healthy population. In common with some other studies antigens HL-A13 (HLA-B13), W15 (HLA-BW15) and W21 (HLA-BW21) were found in excess but these became non-significant after correction for the number of antigens studied. 68% of patients who had guttate psoriasis de novo, subsequently developed persistent plaque psoriasis.", "contents": "HL-A antigens in patients with guttate psoriasis. Tissue typing was performed on lymphocytes of sixty-two patients with guttate psoriasis, in forty-four of whom this was the first manifestations of the disease. In 84% of cases, the guttate psoriasis was preceded by a clinical infection. A highly significant excess of antigen W17 (HLA-BW17) was found in patients when compared with healthy population. In common with some other studies antigens HL-A13 (HLA-B13), W15 (HLA-BW15) and W21 (HLA-BW21) were found in excess but these became non-significant after correction for the number of antigens studied. 68% of patients who had guttate psoriasis de novo, subsequently developed persistent plaque psoriasis.", "PMID": 952752}
{"id": "pubmed23n0032_161", "title": "HL-A antigens in lichen planus.", "content": "A study of fifty-seven patients with lichen planus showed a significant increase in the frequency of HL-A3 (54% compared with 29.7% in a control population). It is likely that there is a gene in the HL-A chromosomal region increasing the susceptibility to lichen planus. No correlation was found between HL-A3 and age of onset, sex, and presence or absence of mucosal lesions.", "contents": "HL-A antigens in lichen planus. A study of fifty-seven patients with lichen planus showed a significant increase in the frequency of HL-A3 (54% compared with 29.7% in a control population). It is likely that there is a gene in the HL-A chromosomal region increasing the susceptibility to lichen planus. No correlation was found between HL-A3 and age of onset, sex, and presence or absence of mucosal lesions.", "PMID": 952753}
{"id": "pubmed23n0032_162", "title": "HL-A antigens and vitiligo.", "content": "HL-A antigens were found with similar frequency in ninety vitiligo patients and in 341 healthy controls. However there was a significant correlation between HL-A13 and vitiligo with antithyroid antibodies. This supports the concept that vitiligo is a syndrome rather than a disease.", "contents": "HL-A antigens and vitiligo. HL-A antigens were found with similar frequency in ninety vitiligo patients and in 341 healthy controls. However there was a significant correlation between HL-A13 and vitiligo with antithyroid antibodies. This supports the concept that vitiligo is a syndrome rather than a disease.", "PMID": 952754}
{"id": "pubmed23n0032_163", "title": "Erythema multiforme in children. Response to treatment with systemic corticosteroids.", "content": "It is generally accepted that the correct treatment for patients with severe erythema multiforme is systemic corticosteroids. This paper is a review of thirty-two paediatric patients with severe erythema multiforme (Stevens-Johnson syndrome) who were treated with either large doses of systemic corticosteroids or supportive care only. Those patients treated with steroids did not recover sooner than those treated in other fashions and the steroid treated group had a significant incidence of medical complications. This retrospective study proves nothing but it does suggest that treatment of patients with the Stevens-Johnson syndrome with systemic corticosteroids may be associated with significant side effects and prolonged recovery.", "contents": "Erythema multiforme in children. Response to treatment with systemic corticosteroids. It is generally accepted that the correct treatment for patients with severe erythema multiforme is systemic corticosteroids. This paper is a review of thirty-two paediatric patients with severe erythema multiforme (Stevens-Johnson syndrome) who were treated with either large doses of systemic corticosteroids or supportive care only. Those patients treated with steroids did not recover sooner than those treated in other fashions and the steroid treated group had a significant incidence of medical complications. This retrospective study proves nothing but it does suggest that treatment of patients with the Stevens-Johnson syndrome with systemic corticosteroids may be associated with significant side effects and prolonged recovery.", "PMID": 952755}
{"id": "pubmed23n0032_164", "title": "Studies on the effect of salicylic acid on normal skin.", "content": "This investigation was prompted by our ignorance of the way in which salicyclic acid aids desquamation. Salicyclic acid in aqueous cream or white soft paraffin and in concentrations of 2-12% was applied to normal skin of twenty-three subjects while the vehicle alone was applied to the contralateral sites. Biopsies and skin surface biopsies were taken from the test and control sites after 1 week. Histological examination showed that there was less horny layer on the test specimens but that there were no qualitative or quantitative differences in the structure of the viable epidermis. Portions of the biopsies were incubated in the presence of tritiated precursor compounds and subsequent autoradiographic examination showed no difference in the incorporation of thymidine, cytidine or histidine between test and control preparations. Scanning electron microscopy of skin surface biopsies showed some changes in all specimens--presumably due to hydration--but also showed striking differences between test and control sites and were especially marked with higher concentrations of salicylic acid. It is suggested that salicylic acid causes desquamation by dissolution of intercellular cement material.", "contents": "Studies on the effect of salicylic acid on normal skin. This investigation was prompted by our ignorance of the way in which salicyclic acid aids desquamation. Salicyclic acid in aqueous cream or white soft paraffin and in concentrations of 2-12% was applied to normal skin of twenty-three subjects while the vehicle alone was applied to the contralateral sites. Biopsies and skin surface biopsies were taken from the test and control sites after 1 week. Histological examination showed that there was less horny layer on the test specimens but that there were no qualitative or quantitative differences in the structure of the viable epidermis. Portions of the biopsies were incubated in the presence of tritiated precursor compounds and subsequent autoradiographic examination showed no difference in the incorporation of thymidine, cytidine or histidine between test and control preparations. Scanning electron microscopy of skin surface biopsies showed some changes in all specimens--presumably due to hydration--but also showed striking differences between test and control sites and were especially marked with higher concentrations of salicylic acid. It is suggested that salicylic acid causes desquamation by dissolution of intercellular cement material.", "PMID": 952756}
{"id": "pubmed23n0032_165", "title": "Mudi-childhood on the pinnae.", "content": "A case of Mudi-childhood on the pinnae in a young girl is reported.", "contents": "Mudi-childhood on the pinnae. A case of Mudi-childhood on the pinnae in a young girl is reported.", "PMID": 952757}
{"id": "pubmed23n0032_166", "title": "Multiple pigmented naevi in an albino.", "content": "A case of pigmented naevi arising in an albino is described. The histological appearance of a naevus is described in the nature of the pigment discussed.", "contents": "Multiple pigmented naevi in an albino. A case of pigmented naevi arising in an albino is described. The histological appearance of a naevus is described in the nature of the pigment discussed.", "PMID": 952758}
{"id": "pubmed23n0032_167", "title": "Leg ulcers in a family with both beta thalassaemia and glucose-6-phosphate dehydrogenase deficiency.", "content": "A family is presented in which the proposita, affected with thalassaemia major, developed a chronic leg ulcer at the age of 14 years. Her eldest brother, not affected with thalassaemia, had a transient leg ulcer at the age of 18 years and a second brother, affected with thalassaemia minor, developed leg ulcers when aged 15 years. Al three siblings were glucose-6-phosphate dehydrogenase deficient.", "contents": "Leg ulcers in a family with both beta thalassaemia and glucose-6-phosphate dehydrogenase deficiency. A family is presented in which the proposita, affected with thalassaemia major, developed a chronic leg ulcer at the age of 14 years. Her eldest brother, not affected with thalassaemia, had a transient leg ulcer at the age of 18 years and a second brother, affected with thalassaemia minor, developed leg ulcers when aged 15 years. Al three siblings were glucose-6-phosphate dehydrogenase deficient.", "PMID": 952759}
{"id": "pubmed23n0032_168", "title": "Glaucoma following the application of corticosteroid to the skin of the eyelids.", "content": "A case is reported of intractable glaucoma presumed to be associated with the long-term use of a corticosteroid-containing dermatological preparation on the skin of the eyelids for the control of eczema.", "contents": "Glaucoma following the application of corticosteroid to the skin of the eyelids. A case is reported of intractable glaucoma presumed to be associated with the long-term use of a corticosteroid-containing dermatological preparation on the skin of the eyelids for the control of eczema.", "PMID": 952760}
{"id": "pubmed23n0032_169", "title": "Christmas disease (haemophilia B) in a girl with deletion of the short arm of one X-chromosome (functional Turner syndrome).", "content": "A I-year-old girl with severe Christmas disease and a factor IX content less than I% of normal is described. The family history was negative and coagulation studies on her relatives were normal. Genetic investigation showed an XXp-karyotype with deletion of the short arm of one X-chromosome, a cytogenetic variant of Turner syndrome. The transmission pathway of the haemophilia gene is discussed.", "contents": "Christmas disease (haemophilia B) in a girl with deletion of the short arm of one X-chromosome (functional Turner syndrome). A I-year-old girl with severe Christmas disease and a factor IX content less than I% of normal is described. The family history was negative and coagulation studies on her relatives were normal. Genetic investigation showed an XXp-karyotype with deletion of the short arm of one X-chromosome, a cytogenetic variant of Turner syndrome. The transmission pathway of the haemophilia gene is discussed.", "PMID": 952762}
{"id": "pubmed23n0032_170", "title": "Evidence that colagen releases human platelet constituents by two different mechanisms.", "content": "Collagen is believed to be involved in the initial events in haemostasis and has been shown by others to cause platelet aggregation and release, and also to initiate the intrinsic pathway of coagulation. The present experiments provide evidence whihc suggests how these many effects of collagen may be involved in haemostasis. It is shown here that collagen releases platelet constituents by two different pathways. Collagen causes platelets washed free of loosely adsorbed coagulation factors to release constituents. This activity, is therefore, independent of the intrinsic pathway of coagulation, and is not inhibited by heparin or hirudin. Collagen also releases platelet constituents by an alternative pathway which is inhibited by heparin and hirudin and is independent of factor XII, but is dependent on factor XI, subsequent factors in the intrinsic pathway of coagulation and calcium. These results suggest that collagen-induced release of platelet constituents is in part due to a direct effect on the platelet, and , in part, to an indirect effect involving coagulation factors and mediated by thrombin. The present results suggest that irreversible aggregation by collagen is also mediated by thrombin. The possible significance of this dual action of collagen in the haemostatic process is shown in Fig 7.", "contents": "Evidence that colagen releases human platelet constituents by two different mechanisms. Collagen is believed to be involved in the initial events in haemostasis and has been shown by others to cause platelet aggregation and release, and also to initiate the intrinsic pathway of coagulation. The present experiments provide evidence whihc suggests how these many effects of collagen may be involved in haemostasis. It is shown here that collagen releases platelet constituents by two different pathways. Collagen causes platelets washed free of loosely adsorbed coagulation factors to release constituents. This activity, is therefore, independent of the intrinsic pathway of coagulation, and is not inhibited by heparin or hirudin. Collagen also releases platelet constituents by an alternative pathway which is inhibited by heparin and hirudin and is independent of factor XII, but is dependent on factor XI, subsequent factors in the intrinsic pathway of coagulation and calcium. These results suggest that collagen-induced release of platelet constituents is in part due to a direct effect on the platelet, and , in part, to an indirect effect involving coagulation factors and mediated by thrombin. The present results suggest that irreversible aggregation by collagen is also mediated by thrombin. The possible significance of this dual action of collagen in the haemostatic process is shown in Fig 7.", "PMID": 952763}
{"id": "pubmed23n0032_171", "title": "Another example of an antibody reacting optimally with p red cells.", "content": "An antibody is described that reacted strongly with red cells of the p(Tj(a-)) phenotype and moderately to strongly with cord red cells of the P2 phenotype. The antibody failed to react, or reacted very weakly, with red cells of the P1k and phenotypes and with the red cells of five children of a p individual, ahd reacted weakly with occasional red cell samples from adults of the P2 phenotype. These results suggest that the antibody does not detect a product of the p gene but instead detects a P system precursor substance. In p individuals this P system precursor substance is not altered by the p genes. In individuals of the P1 or P2 phenocursor substance is not altered by the p genes. In individuals of the P1 or P2 phenotype this precursor substance is apparently converted to P1 and/or P antigens by the P1 or P2 genes.", "contents": "Another example of an antibody reacting optimally with p red cells. An antibody is described that reacted strongly with red cells of the p(Tj(a-)) phenotype and moderately to strongly with cord red cells of the P2 phenotype. The antibody failed to react, or reacted very weakly, with red cells of the P1k and phenotypes and with the red cells of five children of a p individual, ahd reacted weakly with occasional red cell samples from adults of the P2 phenotype. These results suggest that the antibody does not detect a product of the p gene but instead detects a P system precursor substance. In p individuals this P system precursor substance is not altered by the p genes. In individuals of the P1 or P2 phenocursor substance is not altered by the p genes. In individuals of the P1 or P2 phenotype this precursor substance is apparently converted to P1 and/or P antigens by the P1 or P2 genes.", "PMID": 952765}
{"id": "pubmed23n0032_172", "title": "Quantitation of ineffective erythropoiesis from the incorporation of [15N] delta-aminolaevulinic acid and [15N] glycine into early labelled bilirubin. I. Normal subjects.", "content": "The incorporation of [15N] glycine into early labelled bilirubin and haemoglobin haem was measured in four haematologically normal subjects, using the clearance of [14C] bilirubin to measure total bilirubin production rate. Hepatic haem turnover was calculated from the incorporation of [15N]=sigma-aminolaevulinic acid into early labelled bilirubin. From the exprimental data previously published data in normal subjects a method is derived for the quantitation of ineffective erythropoiesis which can be applied to similar studies in patients with haematological disorders.", "contents": "Quantitation of ineffective erythropoiesis from the incorporation of [15N] delta-aminolaevulinic acid and [15N] glycine into early labelled bilirubin. I. Normal subjects. The incorporation of [15N] glycine into early labelled bilirubin and haemoglobin haem was measured in four haematologically normal subjects, using the clearance of [14C] bilirubin to measure total bilirubin production rate. Hepatic haem turnover was calculated from the incorporation of [15N]=sigma-aminolaevulinic acid into early labelled bilirubin. From the exprimental data previously published data in normal subjects a method is derived for the quantitation of ineffective erythropoiesis which can be applied to similar studies in patients with haematological disorders.", "PMID": 952766}
{"id": "pubmed23n0032_173", "title": "Quantitation of ineffective erythropoiesis from the incorporation of [15N] delta-aminolaevulinic acid and [15N] glycin into early labelled bilirubin. II. Anaemic patients.", "content": "The incorporation of [15N]delta-aminolaevulinic acid and [15N glycine into haemoglobin haem and early labelled bilirubin was measured in subjects with various haematological disorders. The clearance of [14C bilirubin was used to measure bilirubin production rate, and the magnitude of the various sources of bilirubin production and the percentage ineffective erythropoiesis were calculated. Ineffective erythropoiesis was found to be a major factor in the production of the anaemia in patients with the following disorders: megaloblastic anaemia associated with the Lesch-Nyhan syndrome, thalassaemia intermedia, sideroblastic anaemia, and the anaemia of chronic disorders. In three patients with iron-deficiency anaemia ineffective erythropoiesis was increased, but was of minor importance in the production of the anaemia, while in two patients with aplastic anaemia and one with macrocytosis of alcoholism there was no increase in ineffective erythropoiesis.", "contents": "Quantitation of ineffective erythropoiesis from the incorporation of [15N] delta-aminolaevulinic acid and [15N] glycin into early labelled bilirubin. II. Anaemic patients. The incorporation of [15N]delta-aminolaevulinic acid and [15N glycine into haemoglobin haem and early labelled bilirubin was measured in subjects with various haematological disorders. The clearance of [14C bilirubin was used to measure bilirubin production rate, and the magnitude of the various sources of bilirubin production and the percentage ineffective erythropoiesis were calculated. Ineffective erythropoiesis was found to be a major factor in the production of the anaemia in patients with the following disorders: megaloblastic anaemia associated with the Lesch-Nyhan syndrome, thalassaemia intermedia, sideroblastic anaemia, and the anaemia of chronic disorders. In three patients with iron-deficiency anaemia ineffective erythropoiesis was increased, but was of minor importance in the production of the anaemia, while in two patients with aplastic anaemia and one with macrocytosis of alcoholism there was no increase in ineffective erythropoiesis.", "PMID": 952767}
{"id": "pubmed23n0032_174", "title": "Anti-Wrb, and other autoantibodies responsible for positive direct antiglobulin tests in 150 individuals.", "content": "Eluates from the red blood cells (and sera whenever free autoantibody was present) of 150 individuals with positive direct antiglobulin tests, have been studied for antibody specificity. Of 87 patients with AIHA, 64 had autoantibodies reacting with all red cell samples including Rhnu11. Of these 64 anti-d1 autoantibodies, two were, and 32 contained, auto-anti-Wrb. Of 33 patients being treated with alphamethyldopa, who had developed positive direct antiglobulin tests, 23 had anti-d1 autoantibodies four of which contained auto-anti-Wrb. Of 30 haematologically normal donors with positive direct antiglobulin tests, 23 had anti-d1 autoantibodies, two of which were, and six of which contained, auto-anti-Wrb. The full specificities of autoantibodies, other than anti-Wrb and anti-d1, in the 150 patients are described, as are the natures of the protein red cell coatings that caused the positive direct antiglobulin tests. The presence of free serum autoantibody as a correlate of the three clinical conditions is reported. Several observations on auto-anti-Wrb are documented. The antibody can cause gross red cell destruction in vivo, but can be benign on other occasions; it occurs with approximately the same frequency in AIHA patients and \"normal\" donors with positive direct antiglobulin tests, but in fewer patients with alphamethydopa induced positive direct antiglobulin tests; it does not activate complement in vivo; and finally it may eventually provide a clue to the aetiology of AIHA.", "contents": "Anti-Wrb, and other autoantibodies responsible for positive direct antiglobulin tests in 150 individuals. Eluates from the red blood cells (and sera whenever free autoantibody was present) of 150 individuals with positive direct antiglobulin tests, have been studied for antibody specificity. Of 87 patients with AIHA, 64 had autoantibodies reacting with all red cell samples including Rhnu11. Of these 64 anti-d1 autoantibodies, two were, and 32 contained, auto-anti-Wrb. Of 33 patients being treated with alphamethyldopa, who had developed positive direct antiglobulin tests, 23 had anti-d1 autoantibodies four of which contained auto-anti-Wrb. Of 30 haematologically normal donors with positive direct antiglobulin tests, 23 had anti-d1 autoantibodies, two of which were, and six of which contained, auto-anti-Wrb. The full specificities of autoantibodies, other than anti-Wrb and anti-d1, in the 150 patients are described, as are the natures of the protein red cell coatings that caused the positive direct antiglobulin tests. The presence of free serum autoantibody as a correlate of the three clinical conditions is reported. Several observations on auto-anti-Wrb are documented. The antibody can cause gross red cell destruction in vivo, but can be benign on other occasions; it occurs with approximately the same frequency in AIHA patients and \"normal\" donors with positive direct antiglobulin tests, but in fewer patients with alphamethydopa induced positive direct antiglobulin tests; it does not activate complement in vivo; and finally it may eventually provide a clue to the aetiology of AIHA.", "PMID": 952768}
{"id": "pubmed23n0032_175", "title": "Unbalanced globin chain synthesis in erythroid precursor cells of heterozygous alpha-thalassaemia.", "content": "Globin biosynthesis was studied in both erythroid precursors and reticulocytes of three individuals with heterozygous alpha-thalassaemia. In contrast to the finding of equal or nearly equal alpha and beta chain synthesis in the marrow of patients with heterozygous beta-thalassaemia previously examined, our studies showed equal degrees of unbalanced globin synthesis in both reticulocytes and nucleated-erythroid cells of alpha-thalassaemia heterozygotes. Greater stability and less susceptibility to proteolysis of the excess beta-chain formed in alpha-thalassaemia may explain our findings.", "contents": "Unbalanced globin chain synthesis in erythroid precursor cells of heterozygous alpha-thalassaemia. Globin biosynthesis was studied in both erythroid precursors and reticulocytes of three individuals with heterozygous alpha-thalassaemia. In contrast to the finding of equal or nearly equal alpha and beta chain synthesis in the marrow of patients with heterozygous beta-thalassaemia previously examined, our studies showed equal degrees of unbalanced globin synthesis in both reticulocytes and nucleated-erythroid cells of alpha-thalassaemia heterozygotes. Greater stability and less susceptibility to proteolysis of the excess beta-chain formed in alpha-thalassaemia may explain our findings.", "PMID": 952769}
{"id": "pubmed23n0032_176", "title": "Simultaneous inheritance of mutant isoenzymes of erythrocyte pyruvate kinase associated with chronic haemolytic anaemia.", "content": "The heterogeneity of pyruvate kinase (PK) deficiency associated with hereditary haemolytic anaemia is emphasized by studies of a kindred harbouring two distinct mutant forms of this enzyme, both of which were kinetically defective with markedly decreased affinities for the substrate, phosphoenolypyruvate. The two isoenzymes, designated PK-Vancouver1 and PK-Vancouver2, were primarily distinguishable from one another by differences in maximum in vitro activities and by variations in response to fructose-I,6-diphosphate activation. When combined in proband erythrocytes to the exclusion of any normal PK, the isoenzymes were associated with a severe chronic haemolytic process with many of the features of PK deficiency of the more common quantitative type. Clinical laboratory screening tests for detecting PK deficiency may be falsely negative or equivocal in such cases.", "contents": "Simultaneous inheritance of mutant isoenzymes of erythrocyte pyruvate kinase associated with chronic haemolytic anaemia. The heterogeneity of pyruvate kinase (PK) deficiency associated with hereditary haemolytic anaemia is emphasized by studies of a kindred harbouring two distinct mutant forms of this enzyme, both of which were kinetically defective with markedly decreased affinities for the substrate, phosphoenolypyruvate. The two isoenzymes, designated PK-Vancouver1 and PK-Vancouver2, were primarily distinguishable from one another by differences in maximum in vitro activities and by variations in response to fructose-I,6-diphosphate activation. When combined in proband erythrocytes to the exclusion of any normal PK, the isoenzymes were associated with a severe chronic haemolytic process with many of the features of PK deficiency of the more common quantitative type. Clinical laboratory screening tests for detecting PK deficiency may be falsely negative or equivocal in such cases.", "PMID": 952770}
{"id": "pubmed23n0032_177", "title": "A convenient and reproducible filtration technique for the determination of erythrocyte flexibility.", "content": "A micro-cell filtration technique is described in which a silver membrane of 3 mum porosity is used. The apparatus consists of a membrane holder and disposable syringe only. A regeneration procedure which allows indefinite re-use of one membrane is described. Filtration proceeds under the hydrostatic pressure of the fluid column only. A locke-albumin solution has been developed as a suspension medium and adapted for cell washing by the addition of fibrinogen and cholesterol. A plot of the volumes of cells filtered against log time shows a linear relationship for normal erythrocytes. The filtered red cells are virtually undamaged. The overall precision of the method of the order of +/-2%.", "contents": "A convenient and reproducible filtration technique for the determination of erythrocyte flexibility. A micro-cell filtration technique is described in which a silver membrane of 3 mum porosity is used. The apparatus consists of a membrane holder and disposable syringe only. A regeneration procedure which allows indefinite re-use of one membrane is described. Filtration proceeds under the hydrostatic pressure of the fluid column only. A locke-albumin solution has been developed as a suspension medium and adapted for cell washing by the addition of fibrinogen and cholesterol. A plot of the volumes of cells filtered against log time shows a linear relationship for normal erythrocytes. The filtered red cells are virtually undamaged. The overall precision of the method of the order of +/-2%.", "PMID": 952771}
{"id": "pubmed23n0032_178", "title": "Primary leucocyte alkaline phosphatase deficiency in an adult with repeated infections.", "content": "An individual with repeated bacterial infections, eczema, hyperimmunoglobulin E, and a primary deficiency of leucocyte alkaline phosphatase is described. The Lap-deficient neutrophils from this patient had marginally deficient bactericidal activity particularly when challenged with high ratios of bacteria per neutrophil. Leucotactic, metabolic and morphologic features of the neutrophils from the patient were normal. Evidence is presented which contrasts this patient's condition with previously described primary or secondary deficiencies of LAP.", "contents": "Primary leucocyte alkaline phosphatase deficiency in an adult with repeated infections. An individual with repeated bacterial infections, eczema, hyperimmunoglobulin E, and a primary deficiency of leucocyte alkaline phosphatase is described. The Lap-deficient neutrophils from this patient had marginally deficient bactericidal activity particularly when challenged with high ratios of bacteria per neutrophil. Leucotactic, metabolic and morphologic features of the neutrophils from the patient were normal. Evidence is presented which contrasts this patient's condition with previously described primary or secondary deficiencies of LAP.", "PMID": 952772}
{"id": "pubmed23n0032_179", "title": "The 'inductograph'--a graph describing the limits of the latent phase of induced labour in low risk situations.", "content": "The construction of an 'inductograph' is described which defines the rates of progress of the latent phase of labour in 80 per cent of a group of patients selected from a 'low risk' category. A standard induction regime was used, and changes in the uterine cervix were assessed quantitatively by a modified form of the Bishop scoring system.", "contents": "The 'inductograph'--a graph describing the limits of the latent phase of induced labour in low risk situations. The construction of an 'inductograph' is described which defines the rates of progress of the latent phase of labour in 80 per cent of a group of patients selected from a 'low risk' category. A standard induction regime was used, and changes in the uterine cervix were assessed quantitatively by a modified form of the Bishop scoring system.", "PMID": 952780}
{"id": "pubmed23n0032_180", "title": "Concentrations of free fatty acids in maternal and umbilical cord blood during elective Caesarean section.", "content": "The concentration of free fatty acids was measured in blood taken from the umbilical artery and vein of 29 infants during elective Caesarean section and compared with levels in maternal venous blood. The mean venous-arterial (v-a) difference was 0.07 mEq/l. There was a direct and significant correlation between the umbilical v-a difference and maternal venous concentrations.", "contents": "Concentrations of free fatty acids in maternal and umbilical cord blood during elective Caesarean section. The concentration of free fatty acids was measured in blood taken from the umbilical artery and vein of 29 infants during elective Caesarean section and compared with levels in maternal venous blood. The mean venous-arterial (v-a) difference was 0.07 mEq/l. There was a direct and significant correlation between the umbilical v-a difference and maternal venous concentrations.", "PMID": 952781}
{"id": "pubmed23n0032_181", "title": "Arterial pressures in non-pregnant women of child-bearing age in Ile-Ife, Nigeria.", "content": "The range of arterial pressures in non-pregnant women between the ages of 15 and 44 years in Ile-Ife, Western Nigeria, has been determined from a randomly selected group of 626 women. The survey showed a low range of arterial pressures in the women. Blood pressures within the group showed a tendency to rise with age except in those who were aged 40 to 44 years, where the small numbers may have yielded the unexpected lower arterial pressure levels. Parity did not seem to have exerted any clear influence on the blood pressures of the subjects. The exception is in the age group 30 to 34 years where increasing parity was associated with a lower systolic blood pressure. The results of the survey suggested that essential hypertension and chronic renal disease were not likely to be significant factors in the high incidence of pre-eclampsia and eclampsia in the community.", "contents": "Arterial pressures in non-pregnant women of child-bearing age in Ile-Ife, Nigeria. The range of arterial pressures in non-pregnant women between the ages of 15 and 44 years in Ile-Ife, Western Nigeria, has been determined from a randomly selected group of 626 women. The survey showed a low range of arterial pressures in the women. Blood pressures within the group showed a tendency to rise with age except in those who were aged 40 to 44 years, where the small numbers may have yielded the unexpected lower arterial pressure levels. Parity did not seem to have exerted any clear influence on the blood pressures of the subjects. The exception is in the age group 30 to 34 years where increasing parity was associated with a lower systolic blood pressure. The results of the survey suggested that essential hypertension and chronic renal disease were not likely to be significant factors in the high incidence of pre-eclampsia and eclampsia in the community.", "PMID": 952782}
{"id": "pubmed23n0032_182", "title": "Arterial pressures in Nigerian women up to mid-pregnancy.", "content": "A group of 497 women whose pregnancies did not exceed 22 weeks and who were booked at the antenatal clinic between 1st August, 1971 and 31st January, 1972 have been included in a survey of arterial pressures in early pregnancy. The arterial pressures showed a low range and confirmed similar previous observations. The blood pressures among the primigravidae in the group were found to be lower than those of their Aberdeen counterparts. It was also found that the arterial pressures were lower than those of comparable non-pregnant women within the same community. The low arterial pressures in early pregnancy did not offer any special advantage in relation to the incidence of pre-eclampsia and eclampsia, diseases known to be prevalent within the community.", "contents": "Arterial pressures in Nigerian women up to mid-pregnancy. A group of 497 women whose pregnancies did not exceed 22 weeks and who were booked at the antenatal clinic between 1st August, 1971 and 31st January, 1972 have been included in a survey of arterial pressures in early pregnancy. The arterial pressures showed a low range and confirmed similar previous observations. The blood pressures among the primigravidae in the group were found to be lower than those of their Aberdeen counterparts. It was also found that the arterial pressures were lower than those of comparable non-pregnant women within the same community. The low arterial pressures in early pregnancy did not offer any special advantage in relation to the incidence of pre-eclampsia and eclampsia, diseases known to be prevalent within the community.", "PMID": 952783}
{"id": "pubmed23n0032_183", "title": "The management and natural history of severe dysplasia and carcinoma in situ of the uterine cervix.", "content": "This report concerns 375 patients seen in Frederiksberg, Denmark, between 1951 and 1972: 103 of them had severe dysplasia and 272 of them carcinoma in situ of the uterine cervix. Thirty patients had no primary treatment: 9 of them developed invasive carcinoma, and 2 of these died; 12 patients had persistent intraepithelial abnormality of the cervix and the other 9 patients showed no abnormality after varying periods of observation. Recurrences after cone biopsy were noted in 9.5 and 10.1 per cent of patients with severe dysplasia and carcinoma in situ respectively, and in 2 per cent of patients who had an in situ carcinoma treated by hysterectomy. This made a total of 15 patients with recurrences. All were ultimately cured although two patients were found to have invasive carcinoma.", "contents": "The management and natural history of severe dysplasia and carcinoma in situ of the uterine cervix. This report concerns 375 patients seen in Frederiksberg, Denmark, between 1951 and 1972: 103 of them had severe dysplasia and 272 of them carcinoma in situ of the uterine cervix. Thirty patients had no primary treatment: 9 of them developed invasive carcinoma, and 2 of these died; 12 patients had persistent intraepithelial abnormality of the cervix and the other 9 patients showed no abnormality after varying periods of observation. Recurrences after cone biopsy were noted in 9.5 and 10.1 per cent of patients with severe dysplasia and carcinoma in situ respectively, and in 2 per cent of patients who had an in situ carcinoma treated by hysterectomy. This made a total of 15 patients with recurrences. All were ultimately cured although two patients were found to have invasive carcinoma.", "PMID": 952784}
{"id": "pubmed23n0032_184", "title": "Treatment and survival in 631 patients with invasive carcinoma of the cervix.", "content": "From 1951 to 1972, a total of 631 cases of invasive carcinoma of the cervix were diagnosed in Frederiksberg, Copenhagen, Denmark. Treatment was either by surgery or radiotherapy, a combination of both being rarely used. The crude five-year survival rates were 99 per cent in stage Ia, 79 per cent in stage Ib, 67 per cent in stage IIa, 45 per cent in stage IIb, 23 per cent in stage III, 5 per cent in stage IV and 63 per cent for all stages. The relative survival rates were respectively 100 per cent, 81 per cent, 73 per cent, 48 per cent, 26 per cent, 5 per cent and 66 per cent. Radical hysterectomy were found more effective than radiotherapy in stage Ib carcinoma of the cervix. This difference was not explained by age but other factors possibly playing a role are considered. The combination of surgery and radiotherapy is discussed and only seems indicated for patients with stage Ib carcinoma and lymph node metastases.", "contents": "Treatment and survival in 631 patients with invasive carcinoma of the cervix. From 1951 to 1972, a total of 631 cases of invasive carcinoma of the cervix were diagnosed in Frederiksberg, Copenhagen, Denmark. Treatment was either by surgery or radiotherapy, a combination of both being rarely used. The crude five-year survival rates were 99 per cent in stage Ia, 79 per cent in stage Ib, 67 per cent in stage IIa, 45 per cent in stage IIb, 23 per cent in stage III, 5 per cent in stage IV and 63 per cent for all stages. The relative survival rates were respectively 100 per cent, 81 per cent, 73 per cent, 48 per cent, 26 per cent, 5 per cent and 66 per cent. Radical hysterectomy were found more effective than radiotherapy in stage Ib carcinoma of the cervix. This difference was not explained by age but other factors possibly playing a role are considered. The combination of surgery and radiotherapy is discussed and only seems indicated for patients with stage Ib carcinoma and lymph node metastases.", "PMID": 952785}
{"id": "pubmed23n0032_185", "title": "Effect of sterilization on menstrual blood loss.", "content": "Menstrual blood loss was measured objectively in 25 women for up to three months before and for six to twelve months after tubal ligation. The operation made no significant difference to menstrual blood loss.", "contents": "Effect of sterilization on menstrual blood loss. Menstrual blood loss was measured objectively in 25 women for up to three months before and for six to twelve months after tubal ligation. The operation made no significant difference to menstrual blood loss.", "PMID": 952786}
{"id": "pubmed23n0032_186", "title": "The distribution of neoplasm in patients dying after treatment of endometrial carcinoma.", "content": "An analysis of 155 patients with endometrial carcinoma, first seen more than five years ago, shows that 31 per cent died with tumour. Trans-coelomic spread of tumour was the most frequent cause of death and occurred in 13.6 per cent of all patients and in 17.1 per cent of those who were operable. A second important cause of death was haematogenous spread of tumour (6.5 per cent). Local pelvic recurrence was the cause of death in 25 per cent of those who did not survive, and local spread was found in 2.2 per cent of operable patients. A rational approach to therapy for endometrial carcinoma is suggested.", "contents": "The distribution of neoplasm in patients dying after treatment of endometrial carcinoma. An analysis of 155 patients with endometrial carcinoma, first seen more than five years ago, shows that 31 per cent died with tumour. Trans-coelomic spread of tumour was the most frequent cause of death and occurred in 13.6 per cent of all patients and in 17.1 per cent of those who were operable. A second important cause of death was haematogenous spread of tumour (6.5 per cent). Local pelvic recurrence was the cause of death in 25 per cent of those who did not survive, and local spread was found in 2.2 per cent of operable patients. A rational approach to therapy for endometrial carcinoma is suggested.", "PMID": 952787}
{"id": "pubmed23n0032_187", "title": "A successful pregnancy following malignant phase hypertension.", "content": "We describe a patient who had a successful pregnancy three years after starting treatment for malignant phase renal hypertension and two years after a nephrectomy for right renal artery thrombosis and an aorto-femoral graft for right common iliac artery thrombosis.", "contents": "A successful pregnancy following malignant phase hypertension. We describe a patient who had a successful pregnancy three years after starting treatment for malignant phase renal hypertension and two years after a nephrectomy for right renal artery thrombosis and an aorto-femoral graft for right common iliac artery thrombosis.", "PMID": 952789}
{"id": "pubmed23n0032_188", "title": "Problems in assessing effects of induction of labour on perinatal mortality.", "content": "Data on births in the Oxford area for 1965 and 1972 are examined, and a model presented for analysing some effects of induction of labour during this period. It is suggested that this model is generally useful in understanding the various possible explanations for an apparent improvement in perinatal mortality figures.", "contents": "Problems in assessing effects of induction of labour on perinatal mortality. Data on births in the Oxford area for 1965 and 1972 are examined, and a model presented for analysing some effects of induction of labour during this period. It is suggested that this model is generally useful in understanding the various possible explanations for an apparent improvement in perinatal mortality figures.", "PMID": 952790}
{"id": "pubmed23n0032_189", "title": "The outcome of pregnancy in former oral contraceptive users.", "content": "As part of a continuing major prospective survey, the outcome of 5530 pregnancies in former oral contraceptive (pill) users was compared with the outcome of 11 009 pregnancies in the control series of women who had never used the pill. In former pill users there was a significantly higher induced abortion rate. There was no increase in the reported spontaneous abortion rate, and there is evidence to suggest that the true spontaneous abortion rate may be materially lower in former pill users. The previous use of oral contraceptives appeared to have no adverse effect on subsequent births.", "contents": "The outcome of pregnancy in former oral contraceptive users. As part of a continuing major prospective survey, the outcome of 5530 pregnancies in former oral contraceptive (pill) users was compared with the outcome of 11 009 pregnancies in the control series of women who had never used the pill. In former pill users there was a significantly higher induced abortion rate. There was no increase in the reported spontaneous abortion rate, and there is evidence to suggest that the true spontaneous abortion rate may be materially lower in former pill users. The previous use of oral contraceptives appeared to have no adverse effect on subsequent births.", "PMID": 952791}
{"id": "pubmed23n0032_190", "title": "Maternal factors associated with fetal chromosomal anomalies in spontaneous abortions.", "content": "The effect is described of maternal factors on the proportion of fetal chromosomal anomalies in a series of 2620 spontaneous abortions, of which 992 specimens were karyotyped. Maternal age was the most important factor associated with a rise in the proportion abnormal, followed by Social Class I or II and the use of oral contraception before conception. The problem of extrapolating from the proportion abnormal to absolute incidence of anomalies is discussed. In the case of increasing maternal age, the evidence suggests that the rise in incidence of spontaneous abortions with age is accounted for by an increased incidence of chromosomally abnormal fetuses. In the case of high social class and a history of oral contraception, the evidence on incidence is scanty and the rise in the proportion abnormal may either reflect a decrease in the abortion rate of chromosomally normal fetuses, or a small increase in the incidence of lethal chromosomal anomalies.", "contents": "Maternal factors associated with fetal chromosomal anomalies in spontaneous abortions. The effect is described of maternal factors on the proportion of fetal chromosomal anomalies in a series of 2620 spontaneous abortions, of which 992 specimens were karyotyped. Maternal age was the most important factor associated with a rise in the proportion abnormal, followed by Social Class I or II and the use of oral contraception before conception. The problem of extrapolating from the proportion abnormal to absolute incidence of anomalies is discussed. In the case of increasing maternal age, the evidence suggests that the rise in incidence of spontaneous abortions with age is accounted for by an increased incidence of chromosomally abnormal fetuses. In the case of high social class and a history of oral contraception, the evidence on incidence is scanty and the rise in the proportion abnormal may either reflect a decrease in the abortion rate of chromosomally normal fetuses, or a small increase in the incidence of lethal chromosomal anomalies.", "PMID": 952792}
{"id": "pubmed23n0032_191", "title": "Changes in fetal and maternal heart rate during abdominal radiography in late pregnancy.", "content": "Continuous recordings of maternal and fetal heart rate were made in 30 antenatal patients during simulated abdominal radiography in late pregnancy. Transient changes in fetal heart rate occurred in 43 per cent of patients in the supine position compared with 27 per cent in the prone position. Transient increase of maternal heart rate occurred in 37 per cent of patients. There was no evidence of revealed or concealed caval occlusion.", "contents": "Changes in fetal and maternal heart rate during abdominal radiography in late pregnancy. Continuous recordings of maternal and fetal heart rate were made in 30 antenatal patients during simulated abdominal radiography in late pregnancy. Transient changes in fetal heart rate occurred in 43 per cent of patients in the supine position compared with 27 per cent in the prone position. Transient increase of maternal heart rate occurred in 37 per cent of patients. There was no evidence of revealed or concealed caval occlusion.", "PMID": 952793}
{"id": "pubmed23n0032_192", "title": "A comparison of the effect of extradural and parenteral analgesia on maternal plasma cortisol concentrations during labour and the puerperium.", "content": "Maternal plasma levels of cortisol were measured serially by radioimmunoassay in two groups of 12 patients during induced labour and in the puerperium. One group was given continuous extradural analgesia throughout labour, the other group received pethidine and promazine in response to pain. Pre-induction cortisol levels were significantly higher in patients who were to have extradural analgesia but the percentage increase in plasma cortisol during labour was considerably less than in patients with parenteral analgesia; in second stage labour, mean cortisol levels were the same in the two groups. This study suggests that patients who had chosen to have extradural analgesia may have been more anxious before labour than the other patients but continuous, extradural analgesia suppressed to some extent the percentage increase in mean cortisol levels found during labour in patients given parenteral analgesia. In the puerperium, there was no difference in mean cortisol levels in the two groups.", "contents": "A comparison of the effect of extradural and parenteral analgesia on maternal plasma cortisol concentrations during labour and the puerperium. Maternal plasma levels of cortisol were measured serially by radioimmunoassay in two groups of 12 patients during induced labour and in the puerperium. One group was given continuous extradural analgesia throughout labour, the other group received pethidine and promazine in response to pain. Pre-induction cortisol levels were significantly higher in patients who were to have extradural analgesia but the percentage increase in plasma cortisol during labour was considerably less than in patients with parenteral analgesia; in second stage labour, mean cortisol levels were the same in the two groups. This study suggests that patients who had chosen to have extradural analgesia may have been more anxious before labour than the other patients but continuous, extradural analgesia suppressed to some extent the percentage increase in mean cortisol levels found during labour in patients given parenteral analgesia. In the puerperium, there was no difference in mean cortisol levels in the two groups.", "PMID": 952794}
{"id": "pubmed23n0032_193", "title": "A serial study of coagulation factors XII, XI and X in plasma in normal pregnancy and in pregnancy complicated by pre-eclampsia.", "content": "A serial study of coagulation factors XII, XI and X in plasma has been carried out on 60 primigravidae, prospectively comparing those who remained normal with those who developed pre-eclampsia. In the normal group of 48 patients, the levels of all factors rose as pregnancy advanced, a secondary increase in factors XI and X occurring in the puerperium. Cord levels of all three factors were depressed. In the pre-eclampsia group of 12 patients, factor XII was significantly higher than in the normal group throughout the study, while factors XI and X were slightly lower. Observed changes failed to support the idea of a strong primary role for the coagulation mechanism in the pathogenesis of pre-eclampsia.", "contents": "A serial study of coagulation factors XII, XI and X in plasma in normal pregnancy and in pregnancy complicated by pre-eclampsia. A serial study of coagulation factors XII, XI and X in plasma has been carried out on 60 primigravidae, prospectively comparing those who remained normal with those who developed pre-eclampsia. In the normal group of 48 patients, the levels of all factors rose as pregnancy advanced, a secondary increase in factors XI and X occurring in the puerperium. Cord levels of all three factors were depressed. In the pre-eclampsia group of 12 patients, factor XII was significantly higher than in the normal group throughout the study, while factors XI and X were slightly lower. Observed changes failed to support the idea of a strong primary role for the coagulation mechanism in the pathogenesis of pre-eclampsia.", "PMID": 952795}
{"id": "pubmed23n0032_194", "title": "Induced abortion and secondary infertility.", "content": "The role of induced (and spontaneous) abortions in the aetiology of secondary sterility was investigated. Obstetric and gynaecologic histories were obtained from 100 women with secondary infertility admitted to the First Department of Obstetrics and Gynaecology of the University of Athens Medical School and to the Division of Fertility and Sterility of that Department. For every patient, an attempt was made to find two healthy control subjects from the same hospital with matching for age, parity, and level of education. Two control subjects each were found for 83 of the index patinets. The relative risk of secondary infertility among women with at least one induced abortion and no spontaneous abortions was 3.4 times that among women without any induced or spontaneous abortions (95 per cent confidence interval 1.38-8.37). The relationship was statistically significant and indicated that in Greece, about 45 per cent of the cases of secondary infertility may be attributable to previous induced abortions.", "contents": "Induced abortion and secondary infertility. The role of induced (and spontaneous) abortions in the aetiology of secondary sterility was investigated. Obstetric and gynaecologic histories were obtained from 100 women with secondary infertility admitted to the First Department of Obstetrics and Gynaecology of the University of Athens Medical School and to the Division of Fertility and Sterility of that Department. For every patient, an attempt was made to find two healthy control subjects from the same hospital with matching for age, parity, and level of education. Two control subjects each were found for 83 of the index patinets. The relative risk of secondary infertility among women with at least one induced abortion and no spontaneous abortions was 3.4 times that among women without any induced or spontaneous abortions (95 per cent confidence interval 1.38-8.37). The relationship was statistically significant and indicated that in Greece, about 45 per cent of the cases of secondary infertility may be attributable to previous induced abortions.", "PMID": 952796}
{"id": "pubmed23n0032_195", "title": "Cervical mucus and identification of the fertile phase of the menstrual cycle.", "content": "Nine healthy fertile women were studied during 29 menstrual cycles. A cervical mucus grading system, assessed by the patient and used in conjunction with basal body temperature, was correlated with plasma levels of luteinizing hormone (LH), oestradiol and plasma progesterone. The results show that a patient can be taught to predict the time of ovulation by observing the changes in the cervical mucus.", "contents": "Cervical mucus and identification of the fertile phase of the menstrual cycle. Nine healthy fertile women were studied during 29 menstrual cycles. A cervical mucus grading system, assessed by the patient and used in conjunction with basal body temperature, was correlated with plasma levels of luteinizing hormone (LH), oestradiol and plasma progesterone. The results show that a patient can be taught to predict the time of ovulation by observing the changes in the cervical mucus.", "PMID": 952797}
{"id": "pubmed23n0032_196", "title": "A study of the use of intermittent serum luteinizing hormone, progesterone and oestradiol measurements for the detection of ovulation.", "content": "The value of luteinizing hormone (LH), progesterone (P) and oestradiol (E2) in serum as an index of ovulation and corpus luteum function was studied in blood samples collected at infrequent intervals during the menstrual cycle from 19 healthy Thai women. A serum P level of more than 3 ng/ml was detectable in 70 to 80 per cent samples obtained between days 20 and 25 and a P peak and/or E2 peak (greater than 150 pg/ml) were found at this time in 85 to 94 per cent of the cycles. In not more than 26 per cent of the cycles was an LH peak (greater than 150 ng/ml-LER-907) detected when all results were considered. A significant P and/or E2 peak on day 22 or 23 occurred in more than 78 per cent of 25 to 33 day cycles, whereas, the P and/or E2 peak was detectable in only 50 per cent of the cycles lasting less than 25 or more than 33 days. The postovulatory serum P levels were the ones most consistently raised in patients studied over several consecutive cycles.", "contents": "A study of the use of intermittent serum luteinizing hormone, progesterone and oestradiol measurements for the detection of ovulation. The value of luteinizing hormone (LH), progesterone (P) and oestradiol (E2) in serum as an index of ovulation and corpus luteum function was studied in blood samples collected at infrequent intervals during the menstrual cycle from 19 healthy Thai women. A serum P level of more than 3 ng/ml was detectable in 70 to 80 per cent samples obtained between days 20 and 25 and a P peak and/or E2 peak (greater than 150 pg/ml) were found at this time in 85 to 94 per cent of the cycles. In not more than 26 per cent of the cycles was an LH peak (greater than 150 ng/ml-LER-907) detected when all results were considered. A significant P and/or E2 peak on day 22 or 23 occurred in more than 78 per cent of 25 to 33 day cycles, whereas, the P and/or E2 peak was detectable in only 50 per cent of the cycles lasting less than 25 or more than 33 days. The postovulatory serum P levels were the ones most consistently raised in patients studied over several consecutive cycles.", "PMID": 952798}
{"id": "pubmed23n0032_197", "title": "Chorionic haemangiomata and abruptio placentae. Case report and review.", "content": "A case of fetal death from abruptio placentae is reported in which the placenta was the seat of multiple chorangiomata. This association is believed not to have been fortuitous and a possible mechanism is suggested by which chorangioma may contribute to the pathogenesis of placental abruption. The incidence, morphological variation and clinical manifestations of placental chorangiomata are briefly discussed.", "contents": "Chorionic haemangiomata and abruptio placentae. Case report and review. A case of fetal death from abruptio placentae is reported in which the placenta was the seat of multiple chorangiomata. This association is believed not to have been fortuitous and a possible mechanism is suggested by which chorangioma may contribute to the pathogenesis of placental abruption. The incidence, morphological variation and clinical manifestations of placental chorangiomata are briefly discussed.", "PMID": 952800}
{"id": "pubmed23n0032_198", "title": "Pathology of practolol-induced ocular toxicity.", "content": "The ocular side-effects of prolonged practolol administration concern the cornea and conjunctiva and are related to deficient tear secretion and the formation of an autoantibody which has an affinity for the intercellular zones of squanmous epithelium. Histopathological study of six cases, including a review of the necropsy findings in two, showed destruction of lacrimal gland tissue, epidermalization of the conjunctival epithelium, with epitheliolysis and stromal ulceration of the cornea leading to perforation in two patients. Immunoperoxidase studies showed fixation of specific antibody in the corneal and conjunctival epithelium but, in the one case in which the tissue could be adequately studied, complement fixation could not be demonstrated. Possibly, therefore, the immune response in patients with practolol-induced ocular damage is secondary to the epithelial disturbance rather than its cause.", "contents": "Pathology of practolol-induced ocular toxicity. The ocular side-effects of prolonged practolol administration concern the cornea and conjunctiva and are related to deficient tear secretion and the formation of an autoantibody which has an affinity for the intercellular zones of squanmous epithelium. Histopathological study of six cases, including a review of the necropsy findings in two, showed destruction of lacrimal gland tissue, epidermalization of the conjunctival epithelium, with epitheliolysis and stromal ulceration of the cornea leading to perforation in two patients. Immunoperoxidase studies showed fixation of specific antibody in the corneal and conjunctival epithelium but, in the one case in which the tissue could be adequately studied, complement fixation could not be demonstrated. Possibly, therefore, the immune response in patients with practolol-induced ocular damage is secondary to the epithelial disturbance rather than its cause.", "PMID": 952801}
{"id": "pubmed23n0032_199", "title": "Ageing and degeneration in the macular region: a clinico-pathological study.", "content": "Clinical and pathological examination was performed on 378 eyes from 216 patients aged 43 to 97 years. This series represented eyes in which the fundi were normal or showed various manifestations of senile macular degeneration. The eyes were divided into six groups according to the histological appearance of a linear deposit at the base of the retinal pigment cells. Groups I and II were considered to represent normal ageing, Groups III and IV the progressive development of senile macular degeneration and Groups V and VI the end-results. Group I showed no basal linear deposit. Thickening and hyalinization of Bruch's membrane was noted as early as the fifth decade. Group II showed patchy development of the basal linear deposit in relation to thickened or basophilic segments of Bruch's membrane, or over intercapillary hyalinization extending to the level of the outer surface of the choriocapillaris. Almost all eyes in these two groups retained a normal fundus appearance but visual acuity declined with age even in the absence of other causes. In Group III the basal deposit formed a thin continuous layer associated with moderate degeneration of the retinal pigment epithelium. More than half the eyes had developed a clinical disturbance of pigmentation and in most vision was reduced. Group IV was characterized by thickening of the deposit and more pronounced disturbance of the pigment epithelium. Clinically most eyes showed coarse pigmentary changes and vision was in the order of 6/24. 14-3 per cent of eyes in this group showed early neovascularization from the choroid. In Group V the pigment epithelium disappeared to produce circumscribed areas of depigmentation. The basal linear deposit could be traced throughout the depigmented area in most eyes. Thin fibrovascular sheets were found beneath the pigment epithelium in 41-7 per cent of eyes. Group VI represented disciform degeneration. The basal linear deposit could often be demonstrated as a disrupted hyalinized layer incorporated into the scar. Disciform degeneration was an alternative end-result to geographical atrophy. In each group the clinical and histological findings may be modified by the presence of drusen or by atrophy of the choroid. The basal linear deposit consisted of banded fibres embedded in granular material lying between the plasma infoldings and the basement membrane of the retinal pigment epithelium. This deposit seems to be a manifestation of gradual failure of the pigment epithelium and proved to be the most suitable criterion by which to study the natural history of senile macular degeneration.", "contents": "Ageing and degeneration in the macular region: a clinico-pathological study. Clinical and pathological examination was performed on 378 eyes from 216 patients aged 43 to 97 years. This series represented eyes in which the fundi were normal or showed various manifestations of senile macular degeneration. The eyes were divided into six groups according to the histological appearance of a linear deposit at the base of the retinal pigment cells. Groups I and II were considered to represent normal ageing, Groups III and IV the progressive development of senile macular degeneration and Groups V and VI the end-results. Group I showed no basal linear deposit. Thickening and hyalinization of Bruch's membrane was noted as early as the fifth decade. Group II showed patchy development of the basal linear deposit in relation to thickened or basophilic segments of Bruch's membrane, or over intercapillary hyalinization extending to the level of the outer surface of the choriocapillaris. Almost all eyes in these two groups retained a normal fundus appearance but visual acuity declined with age even in the absence of other causes. In Group III the basal deposit formed a thin continuous layer associated with moderate degeneration of the retinal pigment epithelium. More than half the eyes had developed a clinical disturbance of pigmentation and in most vision was reduced. Group IV was characterized by thickening of the deposit and more pronounced disturbance of the pigment epithelium. Clinically most eyes showed coarse pigmentary changes and vision was in the order of 6/24. 14-3 per cent of eyes in this group showed early neovascularization from the choroid. In Group V the pigment epithelium disappeared to produce circumscribed areas of depigmentation. The basal linear deposit could be traced throughout the depigmented area in most eyes. Thin fibrovascular sheets were found beneath the pigment epithelium in 41-7 per cent of eyes. Group VI represented disciform degeneration. The basal linear deposit could often be demonstrated as a disrupted hyalinized layer incorporated into the scar. Disciform degeneration was an alternative end-result to geographical atrophy. In each group the clinical and histological findings may be modified by the presence of drusen or by atrophy of the choroid. The basal linear deposit consisted of banded fibres embedded in granular material lying between the plasma infoldings and the basement membrane of the retinal pigment epithelium. This deposit seems to be a manifestation of gradual failure of the pigment epithelium and proved to be the most suitable criterion by which to study the natural history of senile macular degeneration.", "PMID": 952802}
{"id": "pubmed23n0032_200", "title": "Unilateral retinitis pigmentosa sine pigmento.", "content": "A patient presented with unilateral findings of night blindness shown by impaired rod function and dark adaptation, constricted visual fields with good central acuity, a barely recordable electro-retinographic b-wave, and a unilaterally impaired electro-oculogram. There were none of the pigmentary changes usually associated with retinitis pigmentosa. The unaffected right eye was normal in all respects. Therefore the case is most probably one of unilateral retinitis pigmentosa sine pigmento.", "contents": "Unilateral retinitis pigmentosa sine pigmento. A patient presented with unilateral findings of night blindness shown by impaired rod function and dark adaptation, constricted visual fields with good central acuity, a barely recordable electro-retinographic b-wave, and a unilaterally impaired electro-oculogram. There were none of the pigmentary changes usually associated with retinitis pigmentosa. The unaffected right eye was normal in all respects. Therefore the case is most probably one of unilateral retinitis pigmentosa sine pigmento.", "PMID": 952804}
{"id": "pubmed23n0032_201", "title": "Ocular findings of elderly cases of homozygous sickle-cell disease in Jamaica.", "content": "The ocular fingings in 60 patients with homozygous sickle-cell disease over the age of 40 years have been described. Peripheral retinal vessel disease was common and appeared to increase with age. Retinitis proliferans was common among older patients in the group. Angioid streaks occurred in 13 (22 per cent) patients.", "contents": "Ocular findings of elderly cases of homozygous sickle-cell disease in Jamaica. The ocular fingings in 60 patients with homozygous sickle-cell disease over the age of 40 years have been described. Peripheral retinal vessel disease was common and appeared to increase with age. Retinitis proliferans was common among older patients in the group. Angioid streaks occurred in 13 (22 per cent) patients.", "PMID": 952805}
{"id": "pubmed23n0032_202", "title": "Ocular toxocariasis in adults.", "content": "Human infection by toxocara larvae has been thought to be predominantly a disease of young children, but the percentage of the population with antibodies to this nematode worm has recently been shown to increase with age. Although a definite diagnosis of ocular toxocariasis can be made only by identifying the larva histologically, the evidence in three cases presenting with recent uniocular loss of vision strongly suggests that adults may also be affected. The lesions noted on ophthalmoscopy closely resembled toxocaral granulomata in children. Toxocaral fluorescent antibody tests were positive in each case.", "contents": "Ocular toxocariasis in adults. Human infection by toxocara larvae has been thought to be predominantly a disease of young children, but the percentage of the population with antibodies to this nematode worm has recently been shown to increase with age. Although a definite diagnosis of ocular toxocariasis can be made only by identifying the larva histologically, the evidence in three cases presenting with recent uniocular loss of vision strongly suggests that adults may also be affected. The lesions noted on ophthalmoscopy closely resembled toxocaral granulomata in children. Toxocaral fluorescent antibody tests were positive in each case.", "PMID": 952806}
{"id": "pubmed23n0032_203", "title": "Synapse formation in retinoblastoma tumours.", "content": "Developing photoreceptor-like cells in retinoblastoma tumour fleurettes were found to contain apical tight junctions, mitochondrial-filled inner segment regions with cilia, and basally located synaptic ribbons and vesicles. Synaptic contract appeared to be established with adjacent neuronal-like elements. We suggest that photoreceptor differentiation in retinoblastoma tumours follows a sequence similar to normal human photoreceptor embryogenesis.", "contents": "Synapse formation in retinoblastoma tumours. Developing photoreceptor-like cells in retinoblastoma tumour fleurettes were found to contain apical tight junctions, mitochondrial-filled inner segment regions with cilia, and basally located synaptic ribbons and vesicles. Synaptic contract appeared to be established with adjacent neuronal-like elements. We suggest that photoreceptor differentiation in retinoblastoma tumours follows a sequence similar to normal human photoreceptor embryogenesis.", "PMID": 952807}
{"id": "pubmed23n0032_204", "title": "Indocyanine green fluorescence angiography of the choroid.", "content": "Indocyanine green fluorescence (ICG) angiography of the choroid gives better visualization of the choroidal vessels than does fluorescein angiography. We found that the detachment of the pigment epithelium seems bigger on ICG than on fluorescein angiograms, and pigmented lesions are more clearly delineated.", "contents": "Indocyanine green fluorescence angiography of the choroid. Indocyanine green fluorescence (ICG) angiography of the choroid gives better visualization of the choroidal vessels than does fluorescein angiography. We found that the detachment of the pigment epithelium seems bigger on ICG than on fluorescein angiograms, and pigmented lesions are more clearly delineated.", "PMID": 952809}
{"id": "pubmed23n0032_205", "title": "Multilobed uveal melanoma masquerading as postoperative choroidal detachment.", "content": "A case of multilobed malignant melanoma with a haemorrhagic retinal detachment which mimicked a postoperative choroidal detachment is described. Because the lesion showed no resolution six weeks after cataract surgery a tumour evaluation was undertaken. The diagnosis was established with a positive radioactive phosphorus uptake (32P) test and biopsy of the intraocular mass. A suggested approach to the diagnosis in such difficult cases is proposed.", "contents": "Multilobed uveal melanoma masquerading as postoperative choroidal detachment. A case of multilobed malignant melanoma with a haemorrhagic retinal detachment which mimicked a postoperative choroidal detachment is described. Because the lesion showed no resolution six weeks after cataract surgery a tumour evaluation was undertaken. The diagnosis was established with a positive radioactive phosphorus uptake (32P) test and biopsy of the intraocular mass. A suggested approach to the diagnosis in such difficult cases is proposed.", "PMID": 952810}
{"id": "pubmed23n0032_206", "title": "Retinal microscope.", "content": "An instrument is described that provides a stable magnified image for ophthalmoscopy and a low power operating microscope combined to allow greatly improved fundal inspection and to eliminate the need to change instruments during detachment surgery.", "contents": "Retinal microscope. An instrument is described that provides a stable magnified image for ophthalmoscopy and a low power operating microscope combined to allow greatly improved fundal inspection and to eliminate the need to change instruments during detachment surgery.", "PMID": 952811}
{"id": "pubmed23n0032_207", "title": "Viscosity and retinal vein thrombosis.", "content": "Whole-blood and plasma viscosity with haematological and biochemical investigations were measured in 44 patients with retinal vein occlusion. The patients were subdivided on the basis of fluorescein angiographic findings into: 1. Those with large areas of capillary non-perfusion. 2. Those with small areas of capillary non-perfusion. 3. Those with an intact capillary pattern. Capillary non-perfusion in retinal vein occlusion is associated with a higher morbidity owing to the complications of retinal neovascularization. Significantly higher values of whole-blood viscosity, packed cell volume, and yield stress have been found in patients with capillary non-perfusion than in those without. These differences may be of critical importance during the episode of retinal vein occlusion and suggest an aetiological factor in the development of capillary non-perfusion. Higher whole-blood and plasma viscosity values and plasma fibrinogen levels have also been shown in the whole retinal vein occlusion group compared with a control group of 30 individuals. These differences may be a factor in the development of retinal vein occlusion but their precise role is difficult to evaluate. Further biochemical investigations in the vein occlusion group supported the strong association with arterial disease and suggested a higher incidence of biochemical abnormalities in those patients with capillary non-perfusion.", "contents": "Viscosity and retinal vein thrombosis. Whole-blood and plasma viscosity with haematological and biochemical investigations were measured in 44 patients with retinal vein occlusion. The patients were subdivided on the basis of fluorescein angiographic findings into: 1. Those with large areas of capillary non-perfusion. 2. Those with small areas of capillary non-perfusion. 3. Those with an intact capillary pattern. Capillary non-perfusion in retinal vein occlusion is associated with a higher morbidity owing to the complications of retinal neovascularization. Significantly higher values of whole-blood viscosity, packed cell volume, and yield stress have been found in patients with capillary non-perfusion than in those without. These differences may be of critical importance during the episode of retinal vein occlusion and suggest an aetiological factor in the development of capillary non-perfusion. Higher whole-blood and plasma viscosity values and plasma fibrinogen levels have also been shown in the whole retinal vein occlusion group compared with a control group of 30 individuals. These differences may be a factor in the development of retinal vein occlusion but their precise role is difficult to evaluate. Further biochemical investigations in the vein occlusion group supported the strong association with arterial disease and suggested a higher incidence of biochemical abnormalities in those patients with capillary non-perfusion.", "PMID": 952813}
{"id": "pubmed23n0032_208", "title": "Fluorescein angiography and its prognostic significance in central retinal vein occlusion.", "content": "To determine the prognostic value of fluorescein angiograms in central retinal occlusion 75 patients presenting within the first three months of their initial visual symptoms were studied prospectively. The prognosis was found to be best (complete or partial resolution) in eyes with good capillary perfusion, an intact perifoveal capillary arcade, and leakage only from terminal venules and veins. A broken perifoveal capillary arcade and much capillary leakage, even in the absence of obvious capillary closure, were precursors of permanent macular oedema. All eyes with extensive capillary closure developed severe complications such as thrombotic glaucoma and neovascularization (pre-retinal fibrosis), and all became blind. Macular transit time and leakage from arteries and veins were not used as prognostic features. Fluorescein angiography during the first month of the initial visual symptoms was of prognostic value only in those cases with poor prognosis. The three-month angiogram was an accurate indicator of the visual outcome in all cases.", "contents": "Fluorescein angiography and its prognostic significance in central retinal vein occlusion. To determine the prognostic value of fluorescein angiograms in central retinal occlusion 75 patients presenting within the first three months of their initial visual symptoms were studied prospectively. The prognosis was found to be best (complete or partial resolution) in eyes with good capillary perfusion, an intact perifoveal capillary arcade, and leakage only from terminal venules and veins. A broken perifoveal capillary arcade and much capillary leakage, even in the absence of obvious capillary closure, were precursors of permanent macular oedema. All eyes with extensive capillary closure developed severe complications such as thrombotic glaucoma and neovascularization (pre-retinal fibrosis), and all became blind. Macular transit time and leakage from arteries and veins were not used as prognostic features. Fluorescein angiography during the first month of the initial visual symptoms was of prognostic value only in those cases with poor prognosis. The three-month angiogram was an accurate indicator of the visual outcome in all cases.", "PMID": 952814}
{"id": "pubmed23n0032_209", "title": "Central retinal artery occlusion (reversible in sickle trait with glaucoma.", "content": "We report a case of central retinal artery occlusion in an 18-year-old black woman with sickle-trait haemoglobinopathy and acute glaucoma after hyphaema. The central retinal artery occlusion occurred immediately after treatment of the glaucoma with osmotic agents, raising the possibility that they played a precipitating role. We suggest that osmotic agents be used with extreme caution in sickle patients with glaucoma. The occlusion was treated by anterior chamber paracentesis with eventual return of good vision. The reversibility of retinal and optic nerve function after total ischaemia is discussed.", "contents": "Central retinal artery occlusion (reversible in sickle trait with glaucoma. We report a case of central retinal artery occlusion in an 18-year-old black woman with sickle-trait haemoglobinopathy and acute glaucoma after hyphaema. The central retinal artery occlusion occurred immediately after treatment of the glaucoma with osmotic agents, raising the possibility that they played a precipitating role. We suggest that osmotic agents be used with extreme caution in sickle patients with glaucoma. The occlusion was treated by anterior chamber paracentesis with eventual return of good vision. The reversibility of retinal and optic nerve function after total ischaemia is discussed.", "PMID": 952815}
{"id": "pubmed23n0032_210", "title": "Macular and perimacular vascular remodelling sickling haemoglobinopathies.", "content": "The posterior pole vasculature of 100 patients with different sickling haemoglobinopathies was studied prospectively over a period of three years. Various abnormalities of the posterior pole vasculature were seen in 29 per cent of the patients. Continuous remodelling of the macular and perimacular vasculature occurred. Visual acuity was variably affected and sometimes remained intact.", "contents": "Macular and perimacular vascular remodelling sickling haemoglobinopathies. The posterior pole vasculature of 100 patients with different sickling haemoglobinopathies was studied prospectively over a period of three years. Various abnormalities of the posterior pole vasculature were seen in 29 per cent of the patients. Continuous remodelling of the macular and perimacular vasculature occurred. Visual acuity was variably affected and sometimes remained intact.", "PMID": 952816}
{"id": "pubmed23n0032_211", "title": "Acute vitreous haemorrhage: a clinical report.", "content": "In the first hours after a vitreous haemorrhage dense enough to obscure the reina, the blood is usually confined to the posthyaloid space in an aqueous phase. Binocular occlusion and elevation provides sufficient immobilization of the eyes in nine out of 10 patients for the blood cells to settle to the bottom of the space and make the retina available for examination and repair. Blood enters the vitreous gel through holes that develop in the posterior hyaloid membrane. Blood in the gel does not settle and requires months to clear.", "contents": "Acute vitreous haemorrhage: a clinical report. In the first hours after a vitreous haemorrhage dense enough to obscure the reina, the blood is usually confined to the posthyaloid space in an aqueous phase. Binocular occlusion and elevation provides sufficient immobilization of the eyes in nine out of 10 patients for the blood cells to settle to the bottom of the space and make the retina available for examination and repair. Blood enters the vitreous gel through holes that develop in the posterior hyaloid membrane. Blood in the gel does not settle and requires months to clear.", "PMID": 952817}
{"id": "pubmed23n0032_212", "title": "Diabetic retinopathy with normal glucose tolerance.", "content": "A case is reported of a patient with typical diabetic retinopathy, but with normal glucose tolerance. Previously reported cases are reviewed and it is concluded that in such patients the diabetes is probably latent, the retinopathy being discovered during a period of natural or induced remission.", "contents": "Diabetic retinopathy with normal glucose tolerance. A case is reported of a patient with typical diabetic retinopathy, but with normal glucose tolerance. Previously reported cases are reviewed and it is concluded that in such patients the diabetes is probably latent, the retinopathy being discovered during a period of natural or induced remission.", "PMID": 952818}
{"id": "pubmed23n0032_213", "title": "Spheroidal degeneration of cornea and conjunctiva.", "content": "A study of almost 1000 outpatients at a London eye hospital showed the presence of asymptomatic yellowish, spheroidal deposits in the peripheral cornea or conjunctiva, or both, in about 6 per cent, with a preponderance of males and older subjects among those affected. This prevalence is less than is observed in people in countries exposed to higher levels of sunlight. Histological study of the deposits showed some tinctorial similarities with pseudoelastic fibres of pingueculae, with which they were sometimes associated, but also significant differences suggesting that the spheroidal deposits might be a composite of degenerate collagen and a second non-collagenous protein. Until a more precise terminology is feasible we suggest that a purely descriptive name such as spheroidal degeneration or droplet keratopathy should be used to describe this entity.", "contents": "Spheroidal degeneration of cornea and conjunctiva. A study of almost 1000 outpatients at a London eye hospital showed the presence of asymptomatic yellowish, spheroidal deposits in the peripheral cornea or conjunctiva, or both, in about 6 per cent, with a preponderance of males and older subjects among those affected. This prevalence is less than is observed in people in countries exposed to higher levels of sunlight. Histological study of the deposits showed some tinctorial similarities with pseudoelastic fibres of pingueculae, with which they were sometimes associated, but also significant differences suggesting that the spheroidal deposits might be a composite of degenerate collagen and a second non-collagenous protein. Until a more precise terminology is feasible we suggest that a purely descriptive name such as spheroidal degeneration or droplet keratopathy should be used to describe this entity.", "PMID": 952819}
{"id": "pubmed23n0032_214", "title": "Contact lenses at high altitude: experience on Everest south-west face 1975.", "content": "Five members of the British Everest Expedition 1975 were equipped with hydrogel contact lenses. Some clinical observations are made about the performance of these lenses. Two men were able to use them up to an altitude of 24,000 ft, where the ambient oxygen tension is of the order of 55 mmHg.", "contents": "Contact lenses at high altitude: experience on Everest south-west face 1975. Five members of the British Everest Expedition 1975 were equipped with hydrogel contact lenses. Some clinical observations are made about the performance of these lenses. Two men were able to use them up to an altitude of 24,000 ft, where the ambient oxygen tension is of the order of 55 mmHg.", "PMID": 952820}
{"id": "pubmed23n0032_215", "title": "Acute endocrine exophthalmos.", "content": "A case is reported of acute endocrine exophthalmos in a 60-year-old clinically euthyroid male diabetic and the rapid response to steroid therapy is described. The exophthalmometer was used to assess objectively the variations of the exophthalmos.", "contents": "Acute endocrine exophthalmos. A case is reported of acute endocrine exophthalmos in a 60-year-old clinically euthyroid male diabetic and the rapid response to steroid therapy is described. The exophthalmometer was used to assess objectively the variations of the exophthalmos.", "PMID": 952821}
{"id": "pubmed23n0032_216", "title": "Retinal vascular changes in congenital hypertrophy of the retinal pigment epithelium.", "content": "The overlying retinal blood vessels were abnormal in five cases of congenital hypertrophy of the retinal pigment epithelium. This illustrated the well-recognized association between outer retinal degeneration and obliteration of the overlying retinal vasculature. The proposed pathophysiological mechanisms, however, seem inadequate to explain completely the morphological changes of the retinal blood vessels in the presence of atrophy of the outer retina.", "contents": "Retinal vascular changes in congenital hypertrophy of the retinal pigment epithelium. The overlying retinal blood vessels were abnormal in five cases of congenital hypertrophy of the retinal pigment epithelium. This illustrated the well-recognized association between outer retinal degeneration and obliteration of the overlying retinal vasculature. The proposed pathophysiological mechanisms, however, seem inadequate to explain completely the morphological changes of the retinal blood vessels in the presence of atrophy of the outer retina.", "PMID": 952822}
{"id": "pubmed23n0032_217", "title": "Retinal vascular changes in bull's eye maculopathy.", "content": "A case of maculopathy mimicking chloroquine toxicity without exposure to the drug is described. The peripheral retina showed sheathing and arterio-venous anastomosis with poor perfusion of the retinal circulation.", "contents": "Retinal vascular changes in bull's eye maculopathy. A case of maculopathy mimicking chloroquine toxicity without exposure to the drug is described. The peripheral retina showed sheathing and arterio-venous anastomosis with poor perfusion of the retinal circulation.", "PMID": 952823}
{"id": "pubmed23n0032_218", "title": "Ocular findings in a case of fucosidosis.", "content": "The ocular findings in a case of fucosidosis are described and the differential diagnosis is discussed. It is suggested that the level of alpha-L-fucosidase should be estimated in cases of bull's eye retinopathy.", "contents": "Ocular findings in a case of fucosidosis. The ocular findings in a case of fucosidosis are described and the differential diagnosis is discussed. It is suggested that the level of alpha-L-fucosidase should be estimated in cases of bull's eye retinopathy.", "PMID": 952824}
{"id": "pubmed23n0032_219", "title": "Annular choroidal sclerosis.", "content": "This is a case description of a rare fundus condition known as annular choriodal sclerosis. No more than four previous cases have so far been reported. In this article consideration is given to the pathogenesis of the disease and a possible familial link.", "contents": "Annular choroidal sclerosis. This is a case description of a rare fundus condition known as annular choriodal sclerosis. No more than four previous cases have so far been reported. In this article consideration is given to the pathogenesis of the disease and a possible familial link.", "PMID": 952825}
{"id": "pubmed23n0032_220", "title": "Aetiology of keratoconus.", "content": "Keratoconus has a common association with atopic conditions. Most keratoconus patients rub their eyes excessively. Eye-rubbing is considered the dominant aetiological factor in two-thirds of patients with keratoconus who progress to contact lens wear. A classification of keratoconus based on the dominant aetiological factors is proposed. No significant psychoneurotic factor was found in the keratoconus patients compared with patients in the control group.", "contents": "Aetiology of keratoconus. Keratoconus has a common association with atopic conditions. Most keratoconus patients rub their eyes excessively. Eye-rubbing is considered the dominant aetiological factor in two-thirds of patients with keratoconus who progress to contact lens wear. A classification of keratoconus based on the dominant aetiological factors is proposed. No significant psychoneurotic factor was found in the keratoconus patients compared with patients in the control group.", "PMID": 952827}
{"id": "pubmed23n0032_221", "title": "Corneal changes in patients treated with clofazimine.", "content": "Fine, brownish lines, similar to chloroquine keratopathy, were observed in two of 19 psoriatic patients given clofazimine. There was no functional disturbance and the changes were reversible.", "contents": "Corneal changes in patients treated with clofazimine. Fine, brownish lines, similar to chloroquine keratopathy, were observed in two of 19 psoriatic patients given clofazimine. There was no functional disturbance and the changes were reversible.", "PMID": 952828}
{"id": "pubmed23n0032_222", "title": "Clinical manifestations secondary to soft contact lens wear.", "content": "The study of 151 patients wearing soft hydrophilic lenses for one year revealed no gross irreversible lesions with loss of vision. But of significance was the presence of superficial lesions of the corneal epithelium, and chronic stromal oedema. While no significant loss of wearing time was noted for women taking the pill, the decrease in tear secretion in those patients with a latent dry eye problem could just alter the balance to produce intolerance. From this report it is the long-term changes to the tissues that remain to be fully investigated for soft hydrophilic lenses.", "contents": "Clinical manifestations secondary to soft contact lens wear. The study of 151 patients wearing soft hydrophilic lenses for one year revealed no gross irreversible lesions with loss of vision. But of significance was the presence of superficial lesions of the corneal epithelium, and chronic stromal oedema. While no significant loss of wearing time was noted for women taking the pill, the decrease in tear secretion in those patients with a latent dry eye problem could just alter the balance to produce intolerance. From this report it is the long-term changes to the tissues that remain to be fully investigated for soft hydrophilic lenses.", "PMID": 952829}
{"id": "pubmed23n0032_223", "title": "Subconjunctival and episcleral lipid deposits.", "content": "Biomicroscopical examination of the bulbar conjunctiva and anterior episclera of 1000 randomly selected outpatients showed the presence of multiple discrete lipid globules in 30 per cent. The lipid deposits were asymptomatic. Their prevalence was age-related, while their distribution and composition were consistent with origin from the conjunctival blood vessels.", "contents": "Subconjunctival and episcleral lipid deposits. Biomicroscopical examination of the bulbar conjunctiva and anterior episclera of 1000 randomly selected outpatients showed the presence of multiple discrete lipid globules in 30 per cent. The lipid deposits were asymptomatic. Their prevalence was age-related, while their distribution and composition were consistent with origin from the conjunctival blood vessels.", "PMID": 952830}
{"id": "pubmed23n0032_224", "title": "Recurrence of astrocytoma of optic nerve after 48 years.", "content": "A tumour presumed to be a juvenile glioma of the optic nerve was removed by a Kronlein procedure from a 4-year old boy in 1925. In 1973 a severe proptosis made it necessary to remove the atrophic eye and a large retro-ocular tumour. Histological examination, supported by electron microscopy, showed the second tumour to be an anaplastic pilocytic astrocytoma. The possible histogenesis and this unusual behaviour pattern are discussed.", "contents": "Recurrence of astrocytoma of optic nerve after 48 years. A tumour presumed to be a juvenile glioma of the optic nerve was removed by a Kronlein procedure from a 4-year old boy in 1925. In 1973 a severe proptosis made it necessary to remove the atrophic eye and a large retro-ocular tumour. Histological examination, supported by electron microscopy, showed the second tumour to be an anaplastic pilocytic astrocytoma. The possible histogenesis and this unusual behaviour pattern are discussed.", "PMID": 952831}
{"id": "pubmed23n0032_225", "title": "Fertilizer calcium as a factor affecting the voluntary intake, digestibility and retention time of pangola grass (Digitaria decumbens) by sheep.", "content": "1. Pangola grass (Digitaria decumbens) grown with and without calcium fertilizer was cut at three stages of regrowth to measure voluntary intake of dry matter (DM) and digestibility of various components of the dried-grass diet by sheep kept in metabolism crates. To determine the extent of a simple Ca deficiency half the sheep on each diet was supplemented with 1-4 g Ca/d. Retention times of the various dietary components in the reticulo-rumen were also determined. 2. Feeding a Ca supplement had no effect on voluntary intake or digestibility. 3. Ca fertilizer increased the Ca content of the grass from 2-2 to 3-8 g/kg DM and DM digestibility from 0-455 to 0-476 (P less than 0-01) due to an increase in the digestibility of the hemicellulose. 4. Voluntary intake was increased from 38-8 to 43-2 g/kg body-weight0.75 per d by Ca fertilizer due to an 18% reduction in the period of time the DM was retained in the reticulorumen. 5. Regressions relating voluntary intake to DM digestibility for the Ca-fertilized and control grass were significantly different (P less than 0-01). When compared at the same DM digestibility the voluntary intake of the Ca-fertilized grass was 2-6 g/kg body-weight0.75 per d higher than that of the control. 6. It was concluded that Ca fertilizer increased both DM digestibility and voluntary intake as a result of changes in the structural composition of the grass and not by a simple increase in the Ca content of the diet.", "contents": "Fertilizer calcium as a factor affecting the voluntary intake, digestibility and retention time of pangola grass (Digitaria decumbens) by sheep. 1. Pangola grass (Digitaria decumbens) grown with and without calcium fertilizer was cut at three stages of regrowth to measure voluntary intake of dry matter (DM) and digestibility of various components of the dried-grass diet by sheep kept in metabolism crates. To determine the extent of a simple Ca deficiency half the sheep on each diet was supplemented with 1-4 g Ca/d. Retention times of the various dietary components in the reticulo-rumen were also determined. 2. Feeding a Ca supplement had no effect on voluntary intake or digestibility. 3. Ca fertilizer increased the Ca content of the grass from 2-2 to 3-8 g/kg DM and DM digestibility from 0-455 to 0-476 (P less than 0-01) due to an increase in the digestibility of the hemicellulose. 4. Voluntary intake was increased from 38-8 to 43-2 g/kg body-weight0.75 per d by Ca fertilizer due to an 18% reduction in the period of time the DM was retained in the reticulorumen. 5. Regressions relating voluntary intake to DM digestibility for the Ca-fertilized and control grass were significantly different (P less than 0-01). When compared at the same DM digestibility the voluntary intake of the Ca-fertilized grass was 2-6 g/kg body-weight0.75 per d higher than that of the control. 6. It was concluded that Ca fertilizer increased both DM digestibility and voluntary intake as a result of changes in the structural composition of the grass and not by a simple increase in the Ca content of the diet.", "PMID": 952832}
{"id": "pubmed23n0032_226", "title": "Amino acid metabolism in the piglet. 2. Influence of fasting on plasma free amino acid concentration and in vivo oxidation of methionine, isoleucine and threonine.", "content": "1. The influence of a 24 h fast on the concentrations of free amino acids in the plasma, and upon the oxidation rates of methionine, isoleucine and threonine was studied (using early weaned, 4-week-old piglets which were receiving a semi-purified diet. 2. There was no change in the total concentration of the essential amino acids as a result of the 24 h fast: the concentration of the branched-chain amino acids increased, but the effect of this was offset by decreases in the concentrations of arginine, histidine, lysine, methionine and phenylalanine. There was a reduction in the concentration of the non-essential amino acids. 3. The piglets received infusions of L-[I-14C]methionine, L-[U-14C]isoleucine and L-[U-14C]-threonine, and the recovery of the label in carbon dioxide was determined. Less than 5% of the activity from methionine was recovered in the CO2 from the fed piglets, whereas 12% was recovered from the fasted piglets. The corresponding values with threonine were 11 and 19% but there was no effect of fasting on the recovery of the label from isoleucine in CO2. 4. The initial dilution of a single dose of a labelled amino acid infused into the bloodstream depends on the plasma concentration of the amino acid. Nutritional regimens may effect the free amino acid concentration in the plasma. Thus comparisons based upon direct determination of activity recovered in CO2 from the labelled dose of an amino acid with animals on different nutritional regimens could not misleading, unless the differences in the concentrations of the amino acid in the plasma are considered.", "contents": "Amino acid metabolism in the piglet. 2. Influence of fasting on plasma free amino acid concentration and in vivo oxidation of methionine, isoleucine and threonine. 1. The influence of a 24 h fast on the concentrations of free amino acids in the plasma, and upon the oxidation rates of methionine, isoleucine and threonine was studied (using early weaned, 4-week-old piglets which were receiving a semi-purified diet. 2. There was no change in the total concentration of the essential amino acids as a result of the 24 h fast: the concentration of the branched-chain amino acids increased, but the effect of this was offset by decreases in the concentrations of arginine, histidine, lysine, methionine and phenylalanine. There was a reduction in the concentration of the non-essential amino acids. 3. The piglets received infusions of L-[I-14C]methionine, L-[U-14C]isoleucine and L-[U-14C]-threonine, and the recovery of the label in carbon dioxide was determined. Less than 5% of the activity from methionine was recovered in the CO2 from the fed piglets, whereas 12% was recovered from the fasted piglets. The corresponding values with threonine were 11 and 19% but there was no effect of fasting on the recovery of the label from isoleucine in CO2. 4. The initial dilution of a single dose of a labelled amino acid infused into the bloodstream depends on the plasma concentration of the amino acid. Nutritional regimens may effect the free amino acid concentration in the plasma. Thus comparisons based upon direct determination of activity recovered in CO2 from the labelled dose of an amino acid with animals on different nutritional regimens could not misleading, unless the differences in the concentrations of the amino acid in the plasma are considered.", "PMID": 952833}
{"id": "pubmed23n0032_227", "title": "Nitrogen metabolism in calves. Effect of giving different amounts of dietary casein with and without formaldehyde treatment.", "content": "I. Calves were given a basal diet of straw and flaked maize (12 g nitrogen/kg dry matter (DM)) or diets with some flaked maize replaced by untreated (UT) casein or formaldehyde-treated (FT) casein to give 19, 29 or 34 g N/kg DM. 2. At all intakes rumen ammonia concentrations were lower and amounts of total-N, non-ammonia-N and amino acid-N entering the duodenum were high when FT-rather the UT-casein supplements were given. 3. Direct measurement of casein entering the duodenum indicated that giving FT rather than UT casein led to much greater amounts of dietary casein escaping degradation in the rumen (70--90% compared to 10--20%). Calculated values for fermentable N indicated that with this low degradability diets containing FT-casein would have provided inadequate N for maximum microbial synthesis in the rumen, and this probably accounted for the marked reduction in amounts of non-casein-N entering the duodenum when FT rather than UT casein was given. 4. Amino acid patterns in duodenal digesta samples after giving the basal diet or diets containing UT-casein were similar. Giving diets containing FT-casein led to changes in this pattern which could sometimes, although not always, be accounted for by estimated differences in proportions of dietary and microbial proteins. 5. At the highest level of N intake FT-casein-supplemented diets led to significantly higher concentrations of most essential amino acids and lower concentrations of most non-essential amino acids in plasma than did UT-casein-supplemented diets. Plasma urea concentrations increased with increasing N intake but were not significantly different for UT- and FT-casein-supplemented diets.", "contents": "Nitrogen metabolism in calves. Effect of giving different amounts of dietary casein with and without formaldehyde treatment. I. Calves were given a basal diet of straw and flaked maize (12 g nitrogen/kg dry matter (DM)) or diets with some flaked maize replaced by untreated (UT) casein or formaldehyde-treated (FT) casein to give 19, 29 or 34 g N/kg DM. 2. At all intakes rumen ammonia concentrations were lower and amounts of total-N, non-ammonia-N and amino acid-N entering the duodenum were high when FT-rather the UT-casein supplements were given. 3. Direct measurement of casein entering the duodenum indicated that giving FT rather than UT casein led to much greater amounts of dietary casein escaping degradation in the rumen (70--90% compared to 10--20%). Calculated values for fermentable N indicated that with this low degradability diets containing FT-casein would have provided inadequate N for maximum microbial synthesis in the rumen, and this probably accounted for the marked reduction in amounts of non-casein-N entering the duodenum when FT rather than UT casein was given. 4. Amino acid patterns in duodenal digesta samples after giving the basal diet or diets containing UT-casein were similar. Giving diets containing FT-casein led to changes in this pattern which could sometimes, although not always, be accounted for by estimated differences in proportions of dietary and microbial proteins. 5. At the highest level of N intake FT-casein-supplemented diets led to significantly higher concentrations of most essential amino acids and lower concentrations of most non-essential amino acids in plasma than did UT-casein-supplemented diets. Plasma urea concentrations increased with increasing N intake but were not significantly different for UT- and FT-casein-supplemented diets.", "PMID": 952834}
{"id": "pubmed23n0032_228", "title": "The ileum of the sheep as a site of protein digestion.", "content": "1. In order to study the ability of the ileum of the sheep to digest and absorb protein, casein or gluten were infused into the intestine of rams through cannulas inserted at distances of 0-05, 7, 9 and 15 m from the pylorus. Amounts of casein or gluten containing 10-20 g nitrogen were infused during 24 h, in animals given a low-N diet. 2. N balance was negative during the preliminary period when no infusion was given; infusion of casein or gluten increased the daily N retention to 5-15 g. 3. Infusion of proteins into the intestine did not induce an increased activity of pancreatic proteases in digesta withdrawn from the lower part of the intestine. 4. The increased N retention after the infusion of proteins into the lower part of the intestine indicates a considerable capacity of this section of the intestine to digest and absorb proteins.", "contents": "The ileum of the sheep as a site of protein digestion. 1. In order to study the ability of the ileum of the sheep to digest and absorb protein, casein or gluten were infused into the intestine of rams through cannulas inserted at distances of 0-05, 7, 9 and 15 m from the pylorus. Amounts of casein or gluten containing 10-20 g nitrogen were infused during 24 h, in animals given a low-N diet. 2. N balance was negative during the preliminary period when no infusion was given; infusion of casein or gluten increased the daily N retention to 5-15 g. 3. Infusion of proteins into the intestine did not induce an increased activity of pancreatic proteases in digesta withdrawn from the lower part of the intestine. 4. The increased N retention after the infusion of proteins into the lower part of the intestine indicates a considerable capacity of this section of the intestine to digest and absorb proteins.", "PMID": 952835}
{"id": "pubmed23n0032_229", "title": "The effect of cold exposure of sheep on digestion, rumen turnover time and efficiency of microbial synthesis.", "content": "1. Six closely shorn sheep were given brome grass (Bromus inermis) pellets at 1 h intervals and maintained at ambient temperatures of --I to Idegree and 18--21degrees for 28 d. Measurements of digestion were made during the last 10 d of temperature exposure. 2. Cold exposure resulted in a reduction in apparent dry matter (DM) digestibility from 0-482 to 0-450, and of apparent digestibility of organic matter (OM) from 0-511 to 0-477. Neither apparent digestibility nor retention of nitrogen was affected. 3. Apparent digestibility of OM in the rumen decreased from 0-300 to 0-242 with cold exposure, and was highly correlated with turnover time in the rumen of 103Ru, which was used as a particulate marker. 4. The efficiency of microbial synthesis (g N incorporated into microbial cells/kg OM apparently digested) was correlated with the dilution rate of the solute marker (51Cr) and with the turnover time of the particulate marker (103Ru) in the rumen. 5. Digestion in the intestine of DM and OM accounted for significantly more of apparent digestion in the whole gastrointestinal tract for sheep kept in the cold than for sheep kept in the warm. The apparent digestibilities of DM and OM entering the intestine were similar in sheep on both treatments, but significantly more non-ammonia-N was digested in the intestines of cold-exposed sheep. 6. The influence of dilution rate of rumen fluid on the efficiency of synthesis of microbial cells in the rumen is discussed.", "contents": "The effect of cold exposure of sheep on digestion, rumen turnover time and efficiency of microbial synthesis. 1. Six closely shorn sheep were given brome grass (Bromus inermis) pellets at 1 h intervals and maintained at ambient temperatures of --I to Idegree and 18--21degrees for 28 d. Measurements of digestion were made during the last 10 d of temperature exposure. 2. Cold exposure resulted in a reduction in apparent dry matter (DM) digestibility from 0-482 to 0-450, and of apparent digestibility of organic matter (OM) from 0-511 to 0-477. Neither apparent digestibility nor retention of nitrogen was affected. 3. Apparent digestibility of OM in the rumen decreased from 0-300 to 0-242 with cold exposure, and was highly correlated with turnover time in the rumen of 103Ru, which was used as a particulate marker. 4. The efficiency of microbial synthesis (g N incorporated into microbial cells/kg OM apparently digested) was correlated with the dilution rate of the solute marker (51Cr) and with the turnover time of the particulate marker (103Ru) in the rumen. 5. Digestion in the intestine of DM and OM accounted for significantly more of apparent digestion in the whole gastrointestinal tract for sheep kept in the cold than for sheep kept in the warm. The apparent digestibilities of DM and OM entering the intestine were similar in sheep on both treatments, but significantly more non-ammonia-N was digested in the intestines of cold-exposed sheep. 6. The influence of dilution rate of rumen fluid on the efficiency of synthesis of microbial cells in the rumen is discussed.", "PMID": 952836}
{"id": "pubmed23n0032_230", "title": "The histological effects of copper and zinc on chick embryo skeletal tissues in organ culture.", "content": "1. The effects of copper and zinc on organ cultures of chick embryo cartilage and bone maintained in low-trace-metal, chemically defined media for up to 8 d were studied macroscopically, histologically and histochemically. Length and wet-weight measurement of explants were assessed statistically. 2. No effects were found with Cu concentrations of 0-5-1-5 mug/ml medium. Between concentrations of 5 and 40 mug Cu/ml medium, lengths and wet-weights of cartilage cultures decreased significantly (P less than 0-001) compared with controls. The decrease was directly proportional to increasing Cu concentration, and that of the length was greater with increasing period of culture (P less than 0-001). 3. With 5--20 mug Cu/ml medium cartilage and bone became yellow in colour, and chondrocytes were swollen, rounded and basophilic. They were detached from their lacunae and the quantity of matrix was reduced. Loss of alkaline phosphatase (EC 3.1.3.1) activity and disappearance of glycogen accompanied the degeneration. Osteogenesis ceased, cells failed to divide and mature, lost their enzymes and died. Cu did not accumulate in the bone matrix. 4. The direct toxic effects of Cu for cartilage and bone may underlie some of the skeletal changes in hepatolenticular degeneration (Wilson's disease). 5. As Zn concentrations were increased from 2-5 to 7-5 mug/ml medium, lengths and wet-weights of cartilaginous cultures were significantly increased (P less than 0-001). As Zn concentrations were further increased (from 10 to 40 mug/ml medium), lengths and wet-weights were significantly decreased (P less than 0-001). 6. Zn stimulated chondrocyte division and vacuolation of cytoplasm. With higher Zn concentrations toxic changes of granular basophilia, lacunar detachment and necrosis were seen. Differentiation and functioning of osteoblasts, osteoclasts and chondroclasts were stimulated by Zn. 7. Zn was found in bone matrix, osteoblasts, osteocytes and hypertrophied chondrocytes.", "contents": "The histological effects of copper and zinc on chick embryo skeletal tissues in organ culture. 1. The effects of copper and zinc on organ cultures of chick embryo cartilage and bone maintained in low-trace-metal, chemically defined media for up to 8 d were studied macroscopically, histologically and histochemically. Length and wet-weight measurement of explants were assessed statistically. 2. No effects were found with Cu concentrations of 0-5-1-5 mug/ml medium. Between concentrations of 5 and 40 mug Cu/ml medium, lengths and wet-weights of cartilage cultures decreased significantly (P less than 0-001) compared with controls. The decrease was directly proportional to increasing Cu concentration, and that of the length was greater with increasing period of culture (P less than 0-001). 3. With 5--20 mug Cu/ml medium cartilage and bone became yellow in colour, and chondrocytes were swollen, rounded and basophilic. They were detached from their lacunae and the quantity of matrix was reduced. Loss of alkaline phosphatase (EC 3.1.3.1) activity and disappearance of glycogen accompanied the degeneration. Osteogenesis ceased, cells failed to divide and mature, lost their enzymes and died. Cu did not accumulate in the bone matrix. 4. The direct toxic effects of Cu for cartilage and bone may underlie some of the skeletal changes in hepatolenticular degeneration (Wilson's disease). 5. As Zn concentrations were increased from 2-5 to 7-5 mug/ml medium, lengths and wet-weights of cartilaginous cultures were significantly increased (P less than 0-001). As Zn concentrations were further increased (from 10 to 40 mug/ml medium), lengths and wet-weights were significantly decreased (P less than 0-001). 6. Zn stimulated chondrocyte division and vacuolation of cytoplasm. With higher Zn concentrations toxic changes of granular basophilia, lacunar detachment and necrosis were seen. Differentiation and functioning of osteoblasts, osteoclasts and chondroclasts were stimulated by Zn. 7. Zn was found in bone matrix, osteoblasts, osteocytes and hypertrophied chondrocytes.", "PMID": 952837}
{"id": "pubmed23n0032_231", "title": "The role of the gut microflora in the utilization of dietary urea by the chick.", "content": "1. In a preliminary experiment, growth of conventional chicks given a basal diet containing adequate amounts of all the essential but none of the non-essential amino acids was improved by supplements of 10-3 g urea or 50-4 g glutamic acid/kg diet or both. 2. In the main study the effects of supplementing the basal diet with 20-6 g urea/kg were compared in groups of sixteen germ-free and conventional chicks. 3. The germ-free chicks did not benefit from the urea supplement whereas the conventional birds showed improved food conversion efficiency and significantly better growth. 4. In both environments nitrogen retention ((mg N intake-mg N excreted)divideg food intake) was higher in the birds given urea, but N utilization ((mg N intake-mg N excreted)dividemg N intake) was reduced. This reduction was greater in the germ-free birds. 5. There was a small increase in plasma ammonia concentration in the germ-free birds given urea but a significantly greater increase in the corresponding conventional group. 6. Plasma uric acid concentrations were variable in both groups, and much lower than the normal range. They followed a similar pattern to the plasma ammonia values. 7. More insoluble N was excreted by the conventional chicks given urea than by the corresponding germ-free group, or by either group given the basal diet. 8. It was concluded that the gut micro-organisms are responsible for the growth-promoting effect of urea, presumably through release of ammonia by bacterial ureas (EC 3.5.1.5) and its consequent incorporation into amino acids.", "contents": "The role of the gut microflora in the utilization of dietary urea by the chick. 1. In a preliminary experiment, growth of conventional chicks given a basal diet containing adequate amounts of all the essential but none of the non-essential amino acids was improved by supplements of 10-3 g urea or 50-4 g glutamic acid/kg diet or both. 2. In the main study the effects of supplementing the basal diet with 20-6 g urea/kg were compared in groups of sixteen germ-free and conventional chicks. 3. The germ-free chicks did not benefit from the urea supplement whereas the conventional birds showed improved food conversion efficiency and significantly better growth. 4. In both environments nitrogen retention ((mg N intake-mg N excreted)divideg food intake) was higher in the birds given urea, but N utilization ((mg N intake-mg N excreted)dividemg N intake) was reduced. This reduction was greater in the germ-free birds. 5. There was a small increase in plasma ammonia concentration in the germ-free birds given urea but a significantly greater increase in the corresponding conventional group. 6. Plasma uric acid concentrations were variable in both groups, and much lower than the normal range. They followed a similar pattern to the plasma ammonia values. 7. More insoluble N was excreted by the conventional chicks given urea than by the corresponding germ-free group, or by either group given the basal diet. 8. It was concluded that the gut micro-organisms are responsible for the growth-promoting effect of urea, presumably through release of ammonia by bacterial ureas (EC 3.5.1.5) and its consequent incorporation into amino acids.", "PMID": 952838}
{"id": "pubmed23n0032_232", "title": "Influence of fructose feeding on individual enzymic reactions in the formation and metabolism of bile acids in rat liver homogenates.", "content": "1. Rats were maintained for 10 d on a semi-synthetic diet containing 700 g glucose or 700 g fructose/kg. Individual enzymic reactions in bile acid synthesis and metabolism were studied by measuring the 7alpha-hydroxylation of [4-14C]cholesterol, and 12alpha-hydroxylation of 7alpha-[6beta-3H]hydroxy-4-cholesten-3-one, the 26-hydroxylation of 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and the 6beta-hydroxylation of [3H]lithocholic acid in liver homogenates. 2. The serum cholesterol level was approximately the same in both groups of animals, but the serum triglyceride level was almost twice as high in the fructose-fed rats compared to the glucose-fed rats. 3. The 6beta-hydroxylation of [3H]ithocholic acid was increased by about 20% in the fructose-fed rats compared to the glucose-fed animals. The activities of the other enzymic reactions studied did not differ significantly between the two groups of animals. The findings are discussed in relation to previous knowledge concerning mechanisms regulating triglyceride, pre-beta-lipoprotein and bile acid synthesis.", "contents": "Influence of fructose feeding on individual enzymic reactions in the formation and metabolism of bile acids in rat liver homogenates. 1. Rats were maintained for 10 d on a semi-synthetic diet containing 700 g glucose or 700 g fructose/kg. Individual enzymic reactions in bile acid synthesis and metabolism were studied by measuring the 7alpha-hydroxylation of [4-14C]cholesterol, and 12alpha-hydroxylation of 7alpha-[6beta-3H]hydroxy-4-cholesten-3-one, the 26-hydroxylation of 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and the 6beta-hydroxylation of [3H]lithocholic acid in liver homogenates. 2. The serum cholesterol level was approximately the same in both groups of animals, but the serum triglyceride level was almost twice as high in the fructose-fed rats compared to the glucose-fed rats. 3. The 6beta-hydroxylation of [3H]ithocholic acid was increased by about 20% in the fructose-fed rats compared to the glucose-fed animals. The activities of the other enzymic reactions studied did not differ significantly between the two groups of animals. The findings are discussed in relation to previous knowledge concerning mechanisms regulating triglyceride, pre-beta-lipoprotein and bile acid synthesis.", "PMID": 952839}
{"id": "pubmed23n0032_233", "title": "A system for automatic collection and removal of urine from male farm animals.", "content": "A system for collection and removal of urine has been developed, consisting of a peristaltic pump controlled by an electronic switch activated by the presence of urine in a funnel and in tubing. For optimum performance, it is necessary to clean parts at regular intervals.", "contents": "A system for automatic collection and removal of urine from male farm animals. A system for collection and removal of urine has been developed, consisting of a peristaltic pump controlled by an electronic switch activated by the presence of urine in a funnel and in tubing. For optimum performance, it is necessary to clean parts at regular intervals.", "PMID": 952840}
{"id": "pubmed23n0032_234", "title": "The copper status of beef cattle in Northern Ireland.", "content": "1. Serum caeruloplasmin (ferroxidase I;EC 1.16.3.1) activity has been used as an indicator of copper status of cattle to survey beef herds in Northern Ireland. 2. The main survey covered some 20 000 cows on 1200 farms. 3. The results are reported in the form of a map showing area where herds of low and very-low Cu status predominate. Clinical signs of Cu deficiency in calves were encountered in only two areas.", "contents": "The copper status of beef cattle in Northern Ireland. 1. Serum caeruloplasmin (ferroxidase I;EC 1.16.3.1) activity has been used as an indicator of copper status of cattle to survey beef herds in Northern Ireland. 2. The main survey covered some 20 000 cows on 1200 farms. 3. The results are reported in the form of a map showing area where herds of low and very-low Cu status predominate. Clinical signs of Cu deficiency in calves were encountered in only two areas.", "PMID": 952841}
{"id": "pubmed23n0032_235", "title": "Energy losses associated with oven-drying and the preparation of rat carcasses for analysis.", "content": "1. The effect of oven-drying on the heat of combustion of rat carcasses was studied and found to produce losses of 10% of the original energy content. Oven-drying also produced a small loss of nitrogen. 2. A method of carcass preparation not involving oven-drying was tested and found to produce suitably homogeneous samples for analysis without any loss of energy.", "contents": "Energy losses associated with oven-drying and the preparation of rat carcasses for analysis. 1. The effect of oven-drying on the heat of combustion of rat carcasses was studied and found to produce losses of 10% of the original energy content. Oven-drying also produced a small loss of nitrogen. 2. A method of carcass preparation not involving oven-drying was tested and found to produce suitably homogeneous samples for analysis without any loss of energy.", "PMID": 952842}
{"id": "pubmed23n0032_236", "title": "The use of pivalic acid as a reference substance in measurements of production of volatile fatty acids by rumen micro-organisms in vitro.", "content": "1. A procedure is described for using pivalic acid as an inert reference substance in determination of changes in concentrations of volatile fatty acids (VFA). 2. Pivalic acid in concentrations of up to 80 mmol/1 had no effect on production of methane or VFA by rumen contents. 3. Pivalic acid was inert during incubation with rumen contents from sheep given different diets and with samples taken at different times with respect to feeding.", "contents": "The use of pivalic acid as a reference substance in measurements of production of volatile fatty acids by rumen micro-organisms in vitro. 1. A procedure is described for using pivalic acid as an inert reference substance in determination of changes in concentrations of volatile fatty acids (VFA). 2. Pivalic acid in concentrations of up to 80 mmol/1 had no effect on production of methane or VFA by rumen contents. 3. Pivalic acid was inert during incubation with rumen contents from sheep given different diets and with samples taken at different times with respect to feeding.", "PMID": 952843}
{"id": "pubmed23n0032_237", "title": "Solution conformation of a retro-D analogue of tocinamide.", "content": "The solution conformation of a retro-D analogue of tocinamide H-D-Cys-D-Asn-D-Gln-D-aIle-D-Tyr-NHCH2CH2S was examined using proton magnetic resonance and circular dichroism spectroscopy. The observations support major contributions to the conformational distribution from structures with a type I beta turn in the sequence D-Asp-D-Gln-D-aIle-D-Tyr. This is topologically similar to the beta turn proposed for oxytocin, L-Tyr-L-Ile-L-Gln-L-Asn, but with the polarity of the CONH groups reversed along the chain; the peptide is, however, hormonally inert. In conjuction with nuclear magnetic resonance data, the circular dichroism spectra are interpreted to indicate that the region of the peptide ring near the disulfide occurs in at least two different conformations. One of the side-chain carboxamides, probably that of asparagine, appears to be intramolecularly associated rather than freely exposed to solvent.", "contents": "Solution conformation of a retro-D analogue of tocinamide. The solution conformation of a retro-D analogue of tocinamide H-D-Cys-D-Asn-D-Gln-D-aIle-D-Tyr-NHCH2CH2S was examined using proton magnetic resonance and circular dichroism spectroscopy. The observations support major contributions to the conformational distribution from structures with a type I beta turn in the sequence D-Asp-D-Gln-D-aIle-D-Tyr. This is topologically similar to the beta turn proposed for oxytocin, L-Tyr-L-Ile-L-Gln-L-Asn, but with the polarity of the CONH groups reversed along the chain; the peptide is, however, hormonally inert. In conjuction with nuclear magnetic resonance data, the circular dichroism spectra are interpreted to indicate that the region of the peptide ring near the disulfide occurs in at least two different conformations. One of the side-chain carboxamides, probably that of asparagine, appears to be intramolecularly associated rather than freely exposed to solvent.", "PMID": 952844}
{"id": "pubmed23n0032_238", "title": "Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems.", "content": "When aqueous solutions of dextran and of poly-(ethylene glycol) are mixed above certain concentrations, immiscible, liquid two-phase systems are obtained which are useful for separating cells by partition. Some salts partition unequally between the phases, giving rise to an electrostatic potential difference between them. Partition of cells has therefore been thought to depend predominantly on membrane charge. We now report two instances in which membrane charge either does not determine or is not the main determinant of cell partition. (A) Cell partition coefficients in phase systems approaching the critical point (the component concentrations below which a homogeneous solution occurs) increase, even in phase systems in which the phase potential difference is practically zero. Furthermore, in such systems, the partition coefficient of (human) erythrocytes is not reduced by complete removal of sialic acid. (B) Rat and mouse erythrocytes have sizable partition coefficients in a phase system away from the critical point with no potential difference between the phases. Cell surface interaction with the polymers is probably responsible for cell partition in these cases. Partition studies on erythrocytes from nine mammalian species in phases near the critical point with and without electrostatic potential differences reveal major species-specific differences in the membrane charge/noncharge components. A correlation has been found, in phases near the critical point that have essentially no electrostatic potential difference, between partition coefficient and the ratio of poly/monounsaturated fatty acids in the membranes of red cells from different species. Our present results thus provide parameters for the separation of cells by partition in addition to or instead of membrane charge depending on the polymer and salt composition and concentration selected.", "contents": "Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems. When aqueous solutions of dextran and of poly-(ethylene glycol) are mixed above certain concentrations, immiscible, liquid two-phase systems are obtained which are useful for separating cells by partition. Some salts partition unequally between the phases, giving rise to an electrostatic potential difference between them. Partition of cells has therefore been thought to depend predominantly on membrane charge. We now report two instances in which membrane charge either does not determine or is not the main determinant of cell partition. (A) Cell partition coefficients in phase systems approaching the critical point (the component concentrations below which a homogeneous solution occurs) increase, even in phase systems in which the phase potential difference is practically zero. Furthermore, in such systems, the partition coefficient of (human) erythrocytes is not reduced by complete removal of sialic acid. (B) Rat and mouse erythrocytes have sizable partition coefficients in a phase system away from the critical point with no potential difference between the phases. Cell surface interaction with the polymers is probably responsible for cell partition in these cases. Partition studies on erythrocytes from nine mammalian species in phases near the critical point with and without electrostatic potential differences reveal major species-specific differences in the membrane charge/noncharge components. A correlation has been found, in phases near the critical point that have essentially no electrostatic potential difference, between partition coefficient and the ratio of poly/monounsaturated fatty acids in the membranes of red cells from different species. Our present results thus provide parameters for the separation of cells by partition in addition to or instead of membrane charge depending on the polymer and salt composition and concentration selected.", "PMID": 952845}
{"id": "pubmed23n0032_239", "title": "Ligand-induced polymerization.", "content": "Three models of ligand-induced polymerization are considered, encompassing dimerization of acceptor-ligand complex and cross-linking of monomer units via a ligand bridge to form either dimer or linear chains. For each model a binding equation is developed and examined in terms of limits, form, and intersection within a family of curves, each member constructed with fixed but different total acceptor concentration. The characteristics of the binding curves that emerge are correlated with those found by examining the dependence on ligand concentration of either the weight-average molecular weight of the acceptor constituent or a function of it. In the latter plots, a maximum exists for the cross-linking models at the same ligand concentration that the corresponding binding curves intersect: the existence of the maximum implies that at saturating concentrations of ligand only monomeric acceptor constituent remains. The practical implication of these findings is discussed in terms of experimental systems reported previously in the literature. In addition, particular comment is made on the forms of binding curves for the three models, which assume shapes generally associated with positive and negative cooperativity. Finally, comparison is made with those systems in which a shift toward polymeric constituent is effected by ligand perturbation of a preexisting association equilibrium.", "contents": "Ligand-induced polymerization. Three models of ligand-induced polymerization are considered, encompassing dimerization of acceptor-ligand complex and cross-linking of monomer units via a ligand bridge to form either dimer or linear chains. For each model a binding equation is developed and examined in terms of limits, form, and intersection within a family of curves, each member constructed with fixed but different total acceptor concentration. The characteristics of the binding curves that emerge are correlated with those found by examining the dependence on ligand concentration of either the weight-average molecular weight of the acceptor constituent or a function of it. In the latter plots, a maximum exists for the cross-linking models at the same ligand concentration that the corresponding binding curves intersect: the existence of the maximum implies that at saturating concentrations of ligand only monomeric acceptor constituent remains. The practical implication of these findings is discussed in terms of experimental systems reported previously in the literature. In addition, particular comment is made on the forms of binding curves for the three models, which assume shapes generally associated with positive and negative cooperativity. Finally, comparison is made with those systems in which a shift toward polymeric constituent is effected by ligand perturbation of a preexisting association equilibrium.", "PMID": 952846}
{"id": "pubmed23n0032_240", "title": "Chromatic models. Interactions between DNA and polypeptides containing L-lysine L-valine: circular dichroism and thermal denaturation studies.", "content": "The interaction of calf thymus DNA with statistical copolymers of L-lysine and L-valine [poly(L-Lys100f-Lvalf)] and block copolymers [poly(L-Lys)100f-poly(L-Val)f] were investigated as a function of ionic strength using circular dichroism (CD) spectroscopy. It was found that valine suppresses the ability of the copolymer-DNA complexes to yield a psi-type CD spectra as found for poly(L-Lys)-DNA [Jordan, C.F., Lerman, L.S., and Venable, J.N. (1972), Nature (london), New Biol. 236, 67] and lowers the ionic strength at which CD distortion occurs. Thermal denaturation, simultaneously monitoring 280-nm ellipticity, [theta]280, and hyperchromicity, h280, was carried out on annealed complexes of poly(L-Lys)-DNA, poly(L-Lys84.5-L-Val15.5)-DNA, poly(L-Lys)87.2-poly(L-Val)12.8-DNA,  and directly mixed complexes of poly(L-Lys)-DNA, IN 2.5 X 10(-4) MEDTA, pH 7.0 solution. The CD denaturation of uncomplexed DNA at several ionic strengths was also determined to examine pre-melting. Despite the inability of both statistical and block copolymers of L-Lys and L-Val to form psi-type complexes with DNA, they bind as well to DNA as does poly(L-Lys) and give rise to a thermal denaturation pattern showing bound peaks between 90 and 100 degrees C, seen clearly with CD denaturation. The thermal denaturation of mixed and annealed complexes of poly(L-Lys)-DNA shows similar patterns in hyperchromicity changes as a function of temperature but very different CD melts. From the CD melt of annealed poly(L-Lys)-DNA, it appears that aggregation and long-range order of the complex are significant in low salt (2.5 X 10(-4) MEDTA) as well as in 1.0 M NaCl. These studies further illustrate the importance of the nature of nonionic interactions (hydrophobic) between polypeptides and DNA in determining the behavior of their complexes, such as causing condensation into higher order asymmetric structures. In light of these observations, the possible significance to the CD melting of chromatin and the validity of identification of C-form DNA by CD spectroscopy are discussed.", "contents": "Chromatic models. Interactions between DNA and polypeptides containing L-lysine L-valine: circular dichroism and thermal denaturation studies. The interaction of calf thymus DNA with statistical copolymers of L-lysine and L-valine [poly(L-Lys100f-Lvalf)] and block copolymers [poly(L-Lys)100f-poly(L-Val)f] were investigated as a function of ionic strength using circular dichroism (CD) spectroscopy. It was found that valine suppresses the ability of the copolymer-DNA complexes to yield a psi-type CD spectra as found for poly(L-Lys)-DNA [Jordan, C.F., Lerman, L.S., and Venable, J.N. (1972), Nature (london), New Biol. 236, 67] and lowers the ionic strength at which CD distortion occurs. Thermal denaturation, simultaneously monitoring 280-nm ellipticity, [theta]280, and hyperchromicity, h280, was carried out on annealed complexes of poly(L-Lys)-DNA, poly(L-Lys84.5-L-Val15.5)-DNA, poly(L-Lys)87.2-poly(L-Val)12.8-DNA,  and directly mixed complexes of poly(L-Lys)-DNA, IN 2.5 X 10(-4) MEDTA, pH 7.0 solution. The CD denaturation of uncomplexed DNA at several ionic strengths was also determined to examine pre-melting. Despite the inability of both statistical and block copolymers of L-Lys and L-Val to form psi-type complexes with DNA, they bind as well to DNA as does poly(L-Lys) and give rise to a thermal denaturation pattern showing bound peaks between 90 and 100 degrees C, seen clearly with CD denaturation. The thermal denaturation of mixed and annealed complexes of poly(L-Lys)-DNA shows similar patterns in hyperchromicity changes as a function of temperature but very different CD melts. From the CD melt of annealed poly(L-Lys)-DNA, it appears that aggregation and long-range order of the complex are significant in low salt (2.5 X 10(-4) MEDTA) as well as in 1.0 M NaCl. These studies further illustrate the importance of the nature of nonionic interactions (hydrophobic) between polypeptides and DNA in determining the behavior of their complexes, such as causing condensation into higher order asymmetric structures. In light of these observations, the possible significance to the CD melting of chromatin and the validity of identification of C-form DNA by CD spectroscopy are discussed.", "PMID": 952847}
{"id": "pubmed23n0032_241", "title": "Angular scattering analysis of the circular dichroism of biological cells. 1. The red blood cell membrane.", "content": "A quantitative interpretation of the distorted circular dichroism spectrum of red blood cell membranes is presented including the effects of the intense small angle scattering and light detection geometry of our spectrophotometers. Corrected spectra have been obtained by nondestructive methods which correspond to an in situ membrane protein conformation of 45% alpha helix, 10% beta sheet, and a conformational precision of better than +/- 10%. Forward scattering calculations which neglect the small angle scattering collected by conventional phototube acceptance angles are shown to be unsuitable for red cell membranes. Ultraviolet refractive index dispersion has been estimated for the membranes together with an evaluation of the sensitivity of the calculated spectrum to this variable. Angular scattering and circular dichroism calculations are extended to two alternative models for the location of hemoglobin in \"pink ghosts\", and it is indicated that this approach might help in resolving the question whether residual hemoglobin is in solution inside the ghost, or is part of its membrane.", "contents": "Angular scattering analysis of the circular dichroism of biological cells. 1. The red blood cell membrane. A quantitative interpretation of the distorted circular dichroism spectrum of red blood cell membranes is presented including the effects of the intense small angle scattering and light detection geometry of our spectrophotometers. Corrected spectra have been obtained by nondestructive methods which correspond to an in situ membrane protein conformation of 45% alpha helix, 10% beta sheet, and a conformational precision of better than +/- 10%. Forward scattering calculations which neglect the small angle scattering collected by conventional phototube acceptance angles are shown to be unsuitable for red cell membranes. Ultraviolet refractive index dispersion has been estimated for the membranes together with an evaluation of the sensitivity of the calculated spectrum to this variable. Angular scattering and circular dichroism calculations are extended to two alternative models for the location of hemoglobin in \"pink ghosts\", and it is indicated that this approach might help in resolving the question whether residual hemoglobin is in solution inside the ghost, or is part of its membrane.", "PMID": 952848}
{"id": "pubmed23n0032_242", "title": "Calculation of absolute rates of RNA synthesis, accumulation, and degradation in tobacco callus in vivo.", "content": "Uptake and incorporation data were used to calculate an absolute rate of ribonucleic acid (RNA) synthesis by assuming that incorporation of radioactive nucleosides into RNA was a first-order function of the nucleoside triphosphate cellular pool. Homogenized tissue was separated into an RNA and cellular pool fraction. Methods were developed to directly measure the specific radioactivity of specific nucleosides in each fraction. Exogenous [3H]uridine was taken up by the cells, converted to I[3H]uridine triphosphate ([3H]UTP) and [3H]cytidine triphosphate ([3H]CTP), and incorporated into RNA. All of the radioactivity in RNA was contained in either uridine or cytidine. The average rate of RNA synthesis (20 mg of RNA (g of RNA)-1h-1) was calculated from changes in the specific radioactivity of uridine or cytidine in RNA and UTP or CTP in the pool. Since ribosomal RNA does not turn over in exponentially growing tissue, its rate of synthesis was measured (14 mg g-1h-1) and compared with the rate of accumulation of RNA (12 mg g-1h-1). The similarity between ribosomal RNA synthesis and total RNA accumulation confirmed the assumption that these methods measured an absolute rate of RNA synthesis.", "contents": "Calculation of absolute rates of RNA synthesis, accumulation, and degradation in tobacco callus in vivo. Uptake and incorporation data were used to calculate an absolute rate of ribonucleic acid (RNA) synthesis by assuming that incorporation of radioactive nucleosides into RNA was a first-order function of the nucleoside triphosphate cellular pool. Homogenized tissue was separated into an RNA and cellular pool fraction. Methods were developed to directly measure the specific radioactivity of specific nucleosides in each fraction. Exogenous [3H]uridine was taken up by the cells, converted to I[3H]uridine triphosphate ([3H]UTP) and [3H]cytidine triphosphate ([3H]CTP), and incorporated into RNA. All of the radioactivity in RNA was contained in either uridine or cytidine. The average rate of RNA synthesis (20 mg of RNA (g of RNA)-1h-1) was calculated from changes in the specific radioactivity of uridine or cytidine in RNA and UTP or CTP in the pool. Since ribosomal RNA does not turn over in exponentially growing tissue, its rate of synthesis was measured (14 mg g-1h-1) and compared with the rate of accumulation of RNA (12 mg g-1h-1). The similarity between ribosomal RNA synthesis and total RNA accumulation confirmed the assumption that these methods measured an absolute rate of RNA synthesis.", "PMID": 952849}
{"id": "pubmed23n0032_243", "title": "Angular scattering analysis of the circular dichroism of biological cells. 2. The red blood cell.", "content": "A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of the major differences between cell and hemoglobin solution spectra were quantitatively accounted for in terms of differential scatter and absorption flattening, with the latter effect dominating the picture. The relatively low sensitivity of the red blood cell suspension circular dichroism spectrum to hemoglobin conformation is due to the order of magnitude flattening of circular dichroism intensity. The importance of accounting for instrumental light detection geometry and the intense small angle scattering (less than 8 degrees) for a range of particle sizes (0.1-5 mum) is made clear.", "contents": "Angular scattering analysis of the circular dichroism of biological cells. 2. The red blood cell. A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of the major differences between cell and hemoglobin solution spectra were quantitatively accounted for in terms of differential scatter and absorption flattening, with the latter effect dominating the picture. The relatively low sensitivity of the red blood cell suspension circular dichroism spectrum to hemoglobin conformation is due to the order of magnitude flattening of circular dichroism intensity. The importance of accounting for instrumental light detection geometry and the intense small angle scattering (less than 8 degrees) for a range of particle sizes (0.1-5 mum) is made clear.", "PMID": 952850}
{"id": "pubmed23n0032_244", "title": "Intramitochondrial localization and proposed metabolic significance of serine transhydroxymethylase.", "content": "Serine transhydroxymethylase is a latent enzyme of intact rat liver mitochondria. The enzyme is neither solubilized by the selective removal of the outer membrane with digitonin, nor inactivated by concentrations of diazobenzenesulfonate that do not penetrate the inner membrane, but that do inhibit solubilized serine transhydroxymethylase. Swelling of mitochondria was studied in isoosmotic solutions of substrates under conditions that would define transport as neutral uniport, anion-hydroxyl exchange, anion-anion exchange, or electrophoretic. L-Serine and glycine appear to be rapidly taken up by a nonelectrophoretic uniport mechanism, while folate and tetrahydrofolate are not tranported. The results localize the enzyme in the matrix and indicate that the latent activity results from a lack of tetrahydrofolate transport across the inner membrane. Based on these results, the dual localization of serine transhydroxymethylase in the mitochondria and the cytosol is proposed to provide a one-carbon shuttle system to link one-carbon metabolism in the two-cellular compartments.", "contents": "Intramitochondrial localization and proposed metabolic significance of serine transhydroxymethylase. Serine transhydroxymethylase is a latent enzyme of intact rat liver mitochondria. The enzyme is neither solubilized by the selective removal of the outer membrane with digitonin, nor inactivated by concentrations of diazobenzenesulfonate that do not penetrate the inner membrane, but that do inhibit solubilized serine transhydroxymethylase. Swelling of mitochondria was studied in isoosmotic solutions of substrates under conditions that would define transport as neutral uniport, anion-hydroxyl exchange, anion-anion exchange, or electrophoretic. L-Serine and glycine appear to be rapidly taken up by a nonelectrophoretic uniport mechanism, while folate and tetrahydrofolate are not tranported. The results localize the enzyme in the matrix and indicate that the latent activity results from a lack of tetrahydrofolate transport across the inner membrane. Based on these results, the dual localization of serine transhydroxymethylase in the mitochondria and the cytosol is proposed to provide a one-carbon shuttle system to link one-carbon metabolism in the two-cellular compartments.", "PMID": 952851}
{"id": "pubmed23n0032_245", "title": "Amyloid A: amphipathic helixes and lipid binding.", "content": "Polypeptide segments, composed of alpha helixes with specific surface topography termed amphipathic helixes, have been proposed as the basic lipid-associating domains of apolipoproteins from the plasma lipoproteins. A computer search for proteins having sequences that could form amphipathic helixes indicated that amyloid A, a pathologically occurring protein usually associated with \"secondary\" amyloidosis, also contained amphipathic helixes. In studies reported here, amyloid A is shown to associate spontaneously with phospholipid vesicles with the following results: (a) the formation of a protein-lipid complex isolated by equilibrium density gradient ultracentrifugation, (b) a 100% increase in alpha helicity as measured by circular dichroism, (c) a 9-nm shift in the fluorescence maximum due to the single tryptophan residue located in the amphipathic region, indicating the tryptophan is moving from a polar to a nonpolar environment, and (d) the formation of stacked disk-like protein-lipid complexes as visualized by negative stain electron microscopy. The temperature dependence of the circular dichroic spectrum of the amyloid A-phospholipid complex suggests that the complex is formed by insertion of protein between the fatty acyl chains of the lipid. These findings suggest that the amphipathic helix is an important structural unit in lipid-associating proteins and that this unit can be recognized on the basis of its amino acid sequence. In addition, these studies have implications for the origin and function of amyloid A protein.", "contents": "Amyloid A: amphipathic helixes and lipid binding. Polypeptide segments, composed of alpha helixes with specific surface topography termed amphipathic helixes, have been proposed as the basic lipid-associating domains of apolipoproteins from the plasma lipoproteins. A computer search for proteins having sequences that could form amphipathic helixes indicated that amyloid A, a pathologically occurring protein usually associated with \"secondary\" amyloidosis, also contained amphipathic helixes. In studies reported here, amyloid A is shown to associate spontaneously with phospholipid vesicles with the following results: (a) the formation of a protein-lipid complex isolated by equilibrium density gradient ultracentrifugation, (b) a 100% increase in alpha helicity as measured by circular dichroism, (c) a 9-nm shift in the fluorescence maximum due to the single tryptophan residue located in the amphipathic region, indicating the tryptophan is moving from a polar to a nonpolar environment, and (d) the formation of stacked disk-like protein-lipid complexes as visualized by negative stain electron microscopy. The temperature dependence of the circular dichroic spectrum of the amyloid A-phospholipid complex suggests that the complex is formed by insertion of protein between the fatty acyl chains of the lipid. These findings suggest that the amphipathic helix is an important structural unit in lipid-associating proteins and that this unit can be recognized on the basis of its amino acid sequence. In addition, these studies have implications for the origin and function of amyloid A protein.", "PMID": 952852}
{"id": "pubmed23n0032_246", "title": "Effects of pressure upon the fluorescence of the riboflavin binding protein and its flavin mononucleotide complex.", "content": "The effect of pressure in the range of 10(-3)-10 kbars upon the ultraviolet fluorescence of the riboflavin binding protein and the fluorescences of its complex with flavin mononucleotide has been studied. The fluorescence spectrum of the isolated protein showed a reversible red shift of 12nm (1000 cm-1) at high pressure, indicating the reversible exposure of the tryptophan to solvent. From the pressure dependence of the visible fluorescence of the protein-flavin complex in the region of 1-4 kbars the volume change in dissociation of the protein-ligand complex was estimated to be +3.3ml/mol. A very sharp increase in fluorescence-up to 30-fold of the low-pressure value-takes place in the region 5-8 kbars. This increase is due to release of the flavin from the complex and is assigned to pressure denaturation of the protein. The midpoint, rho 1/2, of this transition was found at 6.5  kbars and the change in volume, delta, in the reaction (native-to-denatured) was calculated to be -74ml/mol. Addition of up to 30% methanol results in a progressive decrease both in delta and rho 1/2, in agreement with the concept that hydrophobic bonding stabilizes the native structure.", "contents": "Effects of pressure upon the fluorescence of the riboflavin binding protein and its flavin mononucleotide complex. The effect of pressure in the range of 10(-3)-10 kbars upon the ultraviolet fluorescence of the riboflavin binding protein and the fluorescences of its complex with flavin mononucleotide has been studied. The fluorescence spectrum of the isolated protein showed a reversible red shift of 12nm (1000 cm-1) at high pressure, indicating the reversible exposure of the tryptophan to solvent. From the pressure dependence of the visible fluorescence of the protein-flavin complex in the region of 1-4 kbars the volume change in dissociation of the protein-ligand complex was estimated to be +3.3ml/mol. A very sharp increase in fluorescence-up to 30-fold of the low-pressure value-takes place in the region 5-8 kbars. This increase is due to release of the flavin from the complex and is assigned to pressure denaturation of the protein. The midpoint, rho 1/2, of this transition was found at 6.5  kbars and the change in volume, delta, in the reaction (native-to-denatured) was calculated to be -74ml/mol. Addition of up to 30% methanol results in a progressive decrease both in delta and rho 1/2, in agreement with the concept that hydrophobic bonding stabilizes the native structure.", "PMID": 952853}
{"id": "pubmed23n0032_247", "title": "Purification and characterization of the agglutinins from the sponge Axinella polypoides and a study of their combining sites.", "content": "The hemagglutinins from the sponge Axinella polypoides were isolated by affinity chromatography using Sepharose 4B as an absorbent and eluting with DGal. Further separation on DEAE-cellulose and preparative disc electrophoresis on polyacrylamide and agarose gave three fractions. The physicochemical properties and binding specificities of the two main agglutinins were studied. Homogeneity was tested by polyacrylamide electrophoresis and immunoelectrophoresis and by sedimentation analysis. In isoelectric focusing, agglutinin I (mol wt 21 000) showed two bands at pH 3.8 and 3.9. Agglutinin II (mol wt 15 000) showed one band at pH 3.9. Both agglutinins have a carbohydrate content of about 0.5%, are immunochemically unrelated, and differ in amino acid composition. Both precipitate A1, A2, B, Lea, and precursor I blood group substances but to different extents. Inhibition experiments revealed that both agglutinins are inhibited best by terminal nonreducing DGal glycosidically linked beta 1 leads to 6 or by p-nitrophenyl-betaDGal. DGal and DFuc are equally active but about 20 and 12 times less active with agglutinin I and agglutinin II, respectively. DGalNAc and LFuc were inactive even at much higher concentrations. Both agglutinins have similar specificities and react with the immunodominant determinants of blood group B and Lea but not with A and H substances; in A and H substances, reactivity is with side chains in which beta-linked DGal is unsubstituted at the nonreducing terminus. The Axinella polypoides lectins are compared with galactose-specific lectins of different origin and with the aggregation factor system is sponges.", "contents": "Purification and characterization of the agglutinins from the sponge Axinella polypoides and a study of their combining sites. The hemagglutinins from the sponge Axinella polypoides were isolated by affinity chromatography using Sepharose 4B as an absorbent and eluting with DGal. Further separation on DEAE-cellulose and preparative disc electrophoresis on polyacrylamide and agarose gave three fractions. The physicochemical properties and binding specificities of the two main agglutinins were studied. Homogeneity was tested by polyacrylamide electrophoresis and immunoelectrophoresis and by sedimentation analysis. In isoelectric focusing, agglutinin I (mol wt 21 000) showed two bands at pH 3.8 and 3.9. Agglutinin II (mol wt 15 000) showed one band at pH 3.9. Both agglutinins have a carbohydrate content of about 0.5%, are immunochemically unrelated, and differ in amino acid composition. Both precipitate A1, A2, B, Lea, and precursor I blood group substances but to different extents. Inhibition experiments revealed that both agglutinins are inhibited best by terminal nonreducing DGal glycosidically linked beta 1 leads to 6 or by p-nitrophenyl-betaDGal. DGal and DFuc are equally active but about 20 and 12 times less active with agglutinin I and agglutinin II, respectively. DGalNAc and LFuc were inactive even at much higher concentrations. Both agglutinins have similar specificities and react with the immunodominant determinants of blood group B and Lea but not with A and H substances; in A and H substances, reactivity is with side chains in which beta-linked DGal is unsubstituted at the nonreducing terminus. The Axinella polypoides lectins are compared with galactose-specific lectins of different origin and with the aggregation factor system is sponges.", "PMID": 952854}
{"id": "pubmed23n0032_248", "title": "Transcription of isolated mouse liver chromatin.", "content": "Analysis of RNA transcription from isolated mouse liver chromatin has been undertaken by means of RNA-excess hybridizations with small amounts of radioactive DNA. This analysis indicates that mouse liver chromatin is a restricted template for the in vitro synthesis of RNA complements to repetitive DNA, but more RNA species are synthesized than are found in the RNA isolated from mouse liver nuclei. Extraction with 0.5 M NaC1 destroys the template restriction of isolated chromatin. RNA synthesized in vitro from DNA or chromatin templates by Escherichia coli RNA polymerase, as well as in vivo mouse liver nuclear RNA, were each hybridized to 125I-labeled DNA of high, intermediate, or low reiteration frequency. Chromatin-primed and nuclear RNA saturate a smaller portion of each DNA fraction than does DNA-primed RNA. However, chromatin-primed RNA  saturates more high and low reiteration frequency DNA than does nuclear RNA. Simultaneous hybridization of nuclear-and chromatin-primed RNA with 125I-labeled DNA indicates that chromatin-primed RNA contains all of the sequences present in nuclear RNA. Extraction of chromatin with 0.5 MNaC1 leads to removal of histone F1, as well as a wide variety of non-histone proteins. When used as a template for in vitro RNA synthesis, such salt-extracted chromatin produced RNAs that hybridize as large a portion of each DNA fraction  as does DNA-primed RNA.", "contents": "Transcription of isolated mouse liver chromatin. Analysis of RNA transcription from isolated mouse liver chromatin has been undertaken by means of RNA-excess hybridizations with small amounts of radioactive DNA. This analysis indicates that mouse liver chromatin is a restricted template for the in vitro synthesis of RNA complements to repetitive DNA, but more RNA species are synthesized than are found in the RNA isolated from mouse liver nuclei. Extraction with 0.5 M NaC1 destroys the template restriction of isolated chromatin. RNA synthesized in vitro from DNA or chromatin templates by Escherichia coli RNA polymerase, as well as in vivo mouse liver nuclear RNA, were each hybridized to 125I-labeled DNA of high, intermediate, or low reiteration frequency. Chromatin-primed and nuclear RNA saturate a smaller portion of each DNA fraction than does DNA-primed RNA. However, chromatin-primed RNA  saturates more high and low reiteration frequency DNA than does nuclear RNA. Simultaneous hybridization of nuclear-and chromatin-primed RNA with 125I-labeled DNA indicates that chromatin-primed RNA contains all of the sequences present in nuclear RNA. Extraction of chromatin with 0.5 MNaC1 leads to removal of histone F1, as well as a wide variety of non-histone proteins. When used as a template for in vitro RNA synthesis, such salt-extracted chromatin produced RNAs that hybridize as large a portion of each DNA fraction  as does DNA-primed RNA.", "PMID": 952855}
{"id": "pubmed23n0032_249", "title": "Effect of histones and nonhistone chromosomal proteins on the transcription of histone genes from HeLaS3 cell DNA.", "content": "To elucidate the manner in which histones and nonhistone chromosomal proteins interact to render histone genes transcribable in HeLa S3 cells, we have examined transcription of histone mRNA sequences from DNA, as well as from several DNA-chromosomal protein complexes. Histone mRNA sequences were assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNAs. Our results indicate that DNA is an effective template for transcription of histone mRNA sequences and that histones by themselves inhibit transcription from DNA, including transcription of histone genes, in a dose-dependent, nonspecific manner. When complexed with DNA alone, nonhistone chromosomal proteins do not affect the transcription of histone mRNA sequences. However, when associated with DNA in the presence of histones, nonhistone chromosomal proteins are capable of selectively rendering histone genes transcribable. These results suggest a possible role for nonhistone chromosomal proteins in mediating the interactions of histones with DNA to render histone genes transcribable.", "contents": "Effect of histones and nonhistone chromosomal proteins on the transcription of histone genes from HeLaS3 cell DNA. To elucidate the manner in which histones and nonhistone chromosomal proteins interact to render histone genes transcribable in HeLa S3 cells, we have examined transcription of histone mRNA sequences from DNA, as well as from several DNA-chromosomal protein complexes. Histone mRNA sequences were assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNAs. Our results indicate that DNA is an effective template for transcription of histone mRNA sequences and that histones by themselves inhibit transcription from DNA, including transcription of histone genes, in a dose-dependent, nonspecific manner. When complexed with DNA alone, nonhistone chromosomal proteins do not affect the transcription of histone mRNA sequences. However, when associated with DNA in the presence of histones, nonhistone chromosomal proteins are capable of selectively rendering histone genes transcribable. These results suggest a possible role for nonhistone chromosomal proteins in mediating the interactions of histones with DNA to render histone genes transcribable.", "PMID": 952856}
{"id": "pubmed23n0032_250", "title": "Activation of in vitro histone gene transcription from Hela S3 chromatin by S-phase nonhistone chromosomal proteins.", "content": "Using a 3H-labeled single-stranded complementary DNA probe for detection of histone mRNA sequences (Thrall, CL., Park, WD., Rashba, HW., Stein, JL.,Mans, RJ., and Stein, GS.(1974), biochem. Biophys. Res. Commun. 61,1443) we have found that histone genes are transcribed in vitro from chromatin isolated from S-phase HeLa cells but not from chromatin isolated from G1-phase cells (Stein, G., Park W., Thrall, C., Mans, R., and Stein, J. (1975a), Nature (London) 2578 764; Stein, G., Park, W., Thrall, C., Mans, R., Steins, J.(1975b), Biochem. Biophys. Res. Commun. 63, 945). Utilizing the technique of chromatin reconstitution, we have recently demonstrated that it is the nonhistone chromosomal protein portion of the genome that is responsible for this  difference in in vitro histone gene expression (Stein et al., 1975a). In order to determine whether this is attributable to some component of the S-phase chromosomal proteins that promotes the transcriptin of histone genes, a component of the G1 phase chromosomal proteins that inhibits histone gene transcription, or both, in the present study chromatin from both G1 and S-phase cells was dissociated and then reconstituted in the presence of various chromosomal proteins. The results of this study confirm that it is the nonhistone chromosomal proteins that are responsible for the cell cycle stage specific differences in in vitro histone gene expression and further show that these differences can be accounted for by a component or components of the S-phase nonhistone chromosomal proteins that has the capacity, when reconstituted in the presences of G1 phase chromatin, to render the histone genes transcribable in a dose-dependent fashion.", "contents": "Activation of in vitro histone gene transcription from Hela S3 chromatin by S-phase nonhistone chromosomal proteins. Using a 3H-labeled single-stranded complementary DNA probe for detection of histone mRNA sequences (Thrall, CL., Park, WD., Rashba, HW., Stein, JL.,Mans, RJ., and Stein, GS.(1974), biochem. Biophys. Res. Commun. 61,1443) we have found that histone genes are transcribed in vitro from chromatin isolated from S-phase HeLa cells but not from chromatin isolated from G1-phase cells (Stein, G., Park W., Thrall, C., Mans, R., and Stein, J. (1975a), Nature (London) 2578 764; Stein, G., Park, W., Thrall, C., Mans, R., Steins, J.(1975b), Biochem. Biophys. Res. Commun. 63, 945). Utilizing the technique of chromatin reconstitution, we have recently demonstrated that it is the nonhistone chromosomal protein portion of the genome that is responsible for this  difference in in vitro histone gene expression (Stein et al., 1975a). In order to determine whether this is attributable to some component of the S-phase chromosomal proteins that promotes the transcriptin of histone genes, a component of the G1 phase chromosomal proteins that inhibits histone gene transcription, or both, in the present study chromatin from both G1 and S-phase cells was dissociated and then reconstituted in the presence of various chromosomal proteins. The results of this study confirm that it is the nonhistone chromosomal proteins that are responsible for the cell cycle stage specific differences in in vitro histone gene expression and further show that these differences can be accounted for by a component or components of the S-phase nonhistone chromosomal proteins that has the capacity, when reconstituted in the presences of G1 phase chromatin, to render the histone genes transcribable in a dose-dependent fashion.", "PMID": 952857}
{"id": "pubmed23n0032_251", "title": "Ethidium bromide as a probe of conformational heterogeneity of DNA in chromatin. The role of histone H1.", "content": "The accessibility and the tertiary structure of the DNA inside chromatin were studied by using ethidium bromide (EB) as a fluorescent probe. The exclusion model of binding was refined by introductina a parameter alpha (0less than alpha less than 1) which measures the accessibility of the DNA and by taking into account when necessary the existence of two sets of binding sites. We were thus able to fit predicted and experimental isotherms and then to describe completely EB binding to native or partially histone depleted chromatin under various conditions. Itn native chromatin 95% of the DNA (alpha = 0.95) appears to be accessible to EB but two sets of sites are present. The first one corresponds to alpha = 0.13 and is characterized by an affinity constant which is higher by two orders of magnitude than that relative to pure DNA. The second set corresponds to alpha = 0.82 and the corresponding binding constant is only  three or four times lower than that of pure DNA. The sites with high affinity are still present after treatment with formaldehyde but disappear after removal of histon H1. By comparison with chromatin treated with deoxycholate of with artifical complexes between H1 and DNA, high affinity sites were found only when all of the histons are bound to DNA. An alpha value around 0.8 is still obtained in 1 M NaC1 treated chromatin, pointing to the fact that histones H3 and H4 are preventing 20% of the DNA to intercalate EB.", "contents": "Ethidium bromide as a probe of conformational heterogeneity of DNA in chromatin. The role of histone H1. The accessibility and the tertiary structure of the DNA inside chromatin were studied by using ethidium bromide (EB) as a fluorescent probe. The exclusion model of binding was refined by introductina a parameter alpha (0less than alpha less than 1) which measures the accessibility of the DNA and by taking into account when necessary the existence of two sets of binding sites. We were thus able to fit predicted and experimental isotherms and then to describe completely EB binding to native or partially histone depleted chromatin under various conditions. Itn native chromatin 95% of the DNA (alpha = 0.95) appears to be accessible to EB but two sets of sites are present. The first one corresponds to alpha = 0.13 and is characterized by an affinity constant which is higher by two orders of magnitude than that relative to pure DNA. The second set corresponds to alpha = 0.82 and the corresponding binding constant is only  three or four times lower than that of pure DNA. The sites with high affinity are still present after treatment with formaldehyde but disappear after removal of histon H1. By comparison with chromatin treated with deoxycholate of with artifical complexes between H1 and DNA, high affinity sites were found only when all of the histons are bound to DNA. An alpha value around 0.8 is still obtained in 1 M NaC1 treated chromatin, pointing to the fact that histones H3 and H4 are preventing 20% of the DNA to intercalate EB.", "PMID": 952858}
{"id": "pubmed23n0032_252", "title": "Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.", "content": "Micrococcal nuclease has been used to prepare chromatin from HeLa cells and to probe the structure of HeLa chromatin under various ionic conditions and after the removal of chromatin proteins by salt extraction. The results suggest that (1) HeLa chromatin DNA exists as 150-160 base pair beads interspersed with 40-50 base pair bridges;(2) the bead and bridge conformation exists at physiologic salt concentrations; and (3) removal of histone H1 renders the 40-50 base pair bridge, but not the 150-160 base pair bead, more nuclease susceptible.", "contents": "Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes. Micrococcal nuclease has been used to prepare chromatin from HeLa cells and to probe the structure of HeLa chromatin under various ionic conditions and after the removal of chromatin proteins by salt extraction. The results suggest that (1) HeLa chromatin DNA exists as 150-160 base pair beads interspersed with 40-50 base pair bridges;(2) the bead and bridge conformation exists at physiologic salt concentrations; and (3) removal of histone H1 renders the 40-50 base pair bridge, but not the 150-160 base pair bead, more nuclease susceptible.", "PMID": 952859}
{"id": "pubmed23n0032_253", "title": "Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivatives.", "content": "Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting temperature in DNA samples modified by N-AcO-AAF(DNA-AAF) was carefully reinvestigated. From these experiments, we conclude that the melting temperature decrease is equal to 1.15 degree C per percent of modified bases, in DNA-AAF samples. Electric dichroism measurements on sonicated DNA samples modified by the different fluorene derivatives show the fluorene ring perpendicular to the helix axis in the case of the N-AcO-AAF and its fluoro derivative, and lying alone the phosphate-sugar backbone in the case of the iodo derivative. The results presented in this paper, along with those obtained earlier, led us to propose an \"insertion-denaturation model\" for the mode of binding of N-Aco-AAF and its fluoro derivative, and an \"outside binding model\" for the iodo derivative. Discrepancies with the data obtained by Chang et al.((1974) Biochemistry 13,2142-2148) concerning the melting temperature decrease and the electric dichroism results are observed and discussed.", "contents": "Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivatives. Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting temperature in DNA samples modified by N-AcO-AAF(DNA-AAF) was carefully reinvestigated. From these experiments, we conclude that the melting temperature decrease is equal to 1.15 degree C per percent of modified bases, in DNA-AAF samples. Electric dichroism measurements on sonicated DNA samples modified by the different fluorene derivatives show the fluorene ring perpendicular to the helix axis in the case of the N-AcO-AAF and its fluoro derivative, and lying alone the phosphate-sugar backbone in the case of the iodo derivative. The results presented in this paper, along with those obtained earlier, led us to propose an \"insertion-denaturation model\" for the mode of binding of N-Aco-AAF and its fluoro derivative, and an \"outside binding model\" for the iodo derivative. Discrepancies with the data obtained by Chang et al.((1974) Biochemistry 13,2142-2148) concerning the melting temperature decrease and the electric dichroism results are observed and discussed.", "PMID": 952860}
{"id": "pubmed23n0032_254", "title": "Backbone conformational change in the A to B Transition of deoxyribonucleic acid.", "content": "Infrared linear dichroism studies of A-and B-DNA films reveal six bands between 2800 and 3000 cm-1 which must arise from deoxyribose and thymine methyl CH stretching motions. The band at 2890 is perpendicularly polarized in A-DNA but parallel polarized in B-DNA. This band most probably originates in the C'(5)H2 symmetric stretch; the polarization flip is consistent with the structural change occurring at C'(5) during the A to B transition, according to models derived from x-ray work.", "contents": "Backbone conformational change in the A to B Transition of deoxyribonucleic acid. Infrared linear dichroism studies of A-and B-DNA films reveal six bands between 2800 and 3000 cm-1 which must arise from deoxyribose and thymine methyl CH stretching motions. The band at 2890 is perpendicularly polarized in A-DNA but parallel polarized in B-DNA. This band most probably originates in the C'(5)H2 symmetric stretch; the polarization flip is consistent with the structural change occurring at C'(5) during the A to B transition, according to models derived from x-ray work.", "PMID": 952861}
{"id": "pubmed23n0032_255", "title": "Accumulation and decay of messenger ribonucleic acid in mouse kidney.", "content": "The stability of polyadenylated messenger ribonucleic acid(mRNA) from cytoplasmic structures sedimenting faster than 40S was analyzed in normal mouse kidney. Incorporation of radioactivity into poly(A)-containing and poly(A)-lacking cytoplasmic RNAs separated by oligo(dT)-cellulose chromatography was determined after sedimentation of RNA IN SODIUM DODECYL SULFATE CONTAINING  SUCROSE DENSITY GRADIENTS. Radioactivity accumulated in poly(A)-containing RNA during the first 6 h and then decayed exponentially. Beginning 8-12h after administering label, two components were evident in the decay curve of poly(A)-containing RNA; the short-lived component (approximately 57% of newly synthesized molecules) had an apparent half-life of 6h, and the second class (approximately 43% of new mRNA) was more stable, decaying with a 24-h half-life. These studies provide the basis for examining the regulation of mRNA stability during compensatory renal hypertrophy.", "contents": "Accumulation and decay of messenger ribonucleic acid in mouse kidney. The stability of polyadenylated messenger ribonucleic acid(mRNA) from cytoplasmic structures sedimenting faster than 40S was analyzed in normal mouse kidney. Incorporation of radioactivity into poly(A)-containing and poly(A)-lacking cytoplasmic RNAs separated by oligo(dT)-cellulose chromatography was determined after sedimentation of RNA IN SODIUM DODECYL SULFATE CONTAINING  SUCROSE DENSITY GRADIENTS. Radioactivity accumulated in poly(A)-containing RNA during the first 6 h and then decayed exponentially. Beginning 8-12h after administering label, two components were evident in the decay curve of poly(A)-containing RNA; the short-lived component (approximately 57% of newly synthesized molecules) had an apparent half-life of 6h, and the second class (approximately 43% of new mRNA) was more stable, decaying with a 24-h half-life. These studies provide the basis for examining the regulation of mRNA stability during compensatory renal hypertrophy.", "PMID": 952862}
{"id": "pubmed23n0032_256", "title": "The allosteric effect of inositol hexasulfate on oxygen binding by hemoglobin.", "content": "Myoinositol hexasulfate (IHS) is a powerful allosteric effector of oxygen binding by hemoglobin. It binds to deoxyhemoglobin at the same site as 2,3-diphosphoglycerate (DPG) and inositol hexaphosphate (IHP) with an affinity which is intermediate between that of the two phosphate esters. The binding constant calculated from the displacement of the oxygenation curve in the presence of low concentrations of IHS is 0.9X10(6) M. The value obtained directly from the number of protons bound as a function of IHS concentration is 1.0X10(6) M. The agreement between these two independent measurements provides an experimental verification of the empirical equation, relating the oxygen affinity to the binding constants, proposed previously (Benesch, R.E., Benesch, R., Renthal, R and Gratzer, W.B. (1971), Nature (London), New Biol. 2348 174).", "contents": "The allosteric effect of inositol hexasulfate on oxygen binding by hemoglobin. Myoinositol hexasulfate (IHS) is a powerful allosteric effector of oxygen binding by hemoglobin. It binds to deoxyhemoglobin at the same site as 2,3-diphosphoglycerate (DPG) and inositol hexaphosphate (IHP) with an affinity which is intermediate between that of the two phosphate esters. The binding constant calculated from the displacement of the oxygenation curve in the presence of low concentrations of IHS is 0.9X10(6) M. The value obtained directly from the number of protons bound as a function of IHS concentration is 1.0X10(6) M. The agreement between these two independent measurements provides an experimental verification of the empirical equation, relating the oxygen affinity to the binding constants, proposed previously (Benesch, R.E., Benesch, R., Renthal, R and Gratzer, W.B. (1971), Nature (London), New Biol. 2348 174).", "PMID": 952863}
{"id": "pubmed23n0032_257", "title": "Conformational aspects of the interaction of polyanions with liganded beta chains of human hemoglobin.", "content": "The interaction of carbon monoxide beta chains with two allosteric effectors, namely inositol hexaphosphate and benzenehexacarboxylate, was studied. The sedimentation coefficient (s20,w) of the liganded beta chains was measured to be the same both in the presence and absence of the two effectors suggesting that the protein exists as a tetramer under the conditions of our titration and optical studies. The binding of benzenehexacarboxylate to the liganded beta chains was investigated by potentiometric titration in the pH range 6.7-8.0. The results at pH 7.4 showed a binding of 2 mol of benzenehexacarboxylate per tetramer, with an association constant of 1.26 X 10(4) 1. mol-1 at 20 degrees C. The Hill coefficient for the binding was determined to be 0.73. Similar experiments on the interaction of inositol hexaphosphate with the beta chains showed a binding of 2 mol of the effector per tetramer with identical Hill coefficient (0.737) and comparable association constants (0.88 X 10(4)1. mol-1). The value below unity of the Hill coefficient, found for the binding of the two effectors to the protein, probably reflected an anticooperativity produced by the different net electric charges of the free protein and the protein-effector complex. The difference in protons bound per mole of heme by the beta subunits in the presence and absence of benzenehexacarboxylate appeared consistent with the proposal that two groups per chain changed their pK from 6.6 to 7.4 upon the interaction. In the presence of benzenehexacarboxylate, the protonation of these groups appeared to be cooperative, suggesting a conformational change of the protein upon the binding. The absorption spectra of carbon monoxide beta chains in the Soret region was markedly altered by benzenehexacarboxylate and inositol hexaphosphate. The features in the difference spectra of the protein obtained with the two effectors were qualitatively identical and indicated changes in the heme environment produced by the interaction of the effectors with the beta chains. Concomitant changes in circular dichroism and optical rotatory dispersion of the liganded beta chains caused by the addition of the two effectors provided supporting evidence for the conformational change in the protein produced by the binding of the effectors.", "contents": "Conformational aspects of the interaction of polyanions with liganded beta chains of human hemoglobin. The interaction of carbon monoxide beta chains with two allosteric effectors, namely inositol hexaphosphate and benzenehexacarboxylate, was studied. The sedimentation coefficient (s20,w) of the liganded beta chains was measured to be the same both in the presence and absence of the two effectors suggesting that the protein exists as a tetramer under the conditions of our titration and optical studies. The binding of benzenehexacarboxylate to the liganded beta chains was investigated by potentiometric titration in the pH range 6.7-8.0. The results at pH 7.4 showed a binding of 2 mol of benzenehexacarboxylate per tetramer, with an association constant of 1.26 X 10(4) 1. mol-1 at 20 degrees C. The Hill coefficient for the binding was determined to be 0.73. Similar experiments on the interaction of inositol hexaphosphate with the beta chains showed a binding of 2 mol of the effector per tetramer with identical Hill coefficient (0.737) and comparable association constants (0.88 X 10(4)1. mol-1). The value below unity of the Hill coefficient, found for the binding of the two effectors to the protein, probably reflected an anticooperativity produced by the different net electric charges of the free protein and the protein-effector complex. The difference in protons bound per mole of heme by the beta subunits in the presence and absence of benzenehexacarboxylate appeared consistent with the proposal that two groups per chain changed their pK from 6.6 to 7.4 upon the interaction. In the presence of benzenehexacarboxylate, the protonation of these groups appeared to be cooperative, suggesting a conformational change of the protein upon the binding. The absorption spectra of carbon monoxide beta chains in the Soret region was markedly altered by benzenehexacarboxylate and inositol hexaphosphate. The features in the difference spectra of the protein obtained with the two effectors were qualitatively identical and indicated changes in the heme environment produced by the interaction of the effectors with the beta chains. Concomitant changes in circular dichroism and optical rotatory dispersion of the liganded beta chains caused by the addition of the two effectors provided supporting evidence for the conformational change in the protein produced by the binding of the effectors.", "PMID": 952864}
{"id": "pubmed23n0032_258", "title": "Anomalous azide binding to metmanganomyoglobin.", "content": "The reaction of metmanganomyoglobin (MnIIIMb) with azide presents a novel pattern with direct evidence for kinetic complexity. Although the kinetic analysis may not be unique, it appears that the azide complex cannot be fully formed, as judged by spectrophotometric changes, even with an infinitely great [N3-]. This is interpreted as resulting from an equilibrium between the final spectroscopically observable azide complex and an intermediate species whose spectrum is not substantially different from that of MnIIIMb itself. The two forms of the azide complex appear to exhibit roughly equal proportions at 3 degrees C. We propose that this intermediate is a weak Mn3+ -azide complex in which the metal ion remains out-of-plane toward the imidazole of the proximal histidine, but that the metal lies toward the anion in the \"final\" complex.", "contents": "Anomalous azide binding to metmanganomyoglobin. The reaction of metmanganomyoglobin (MnIIIMb) with azide presents a novel pattern with direct evidence for kinetic complexity. Although the kinetic analysis may not be unique, it appears that the azide complex cannot be fully formed, as judged by spectrophotometric changes, even with an infinitely great [N3-]. This is interpreted as resulting from an equilibrium between the final spectroscopically observable azide complex and an intermediate species whose spectrum is not substantially different from that of MnIIIMb itself. The two forms of the azide complex appear to exhibit roughly equal proportions at 3 degrees C. We propose that this intermediate is a weak Mn3+ -azide complex in which the metal ion remains out-of-plane toward the imidazole of the proximal histidine, but that the metal lies toward the anion in the \"final\" complex.", "PMID": 952865}
{"id": "pubmed23n0032_259", "title": "Reinvestigation of some physicochemical and chemical properties of human ceruloplasmin (ferroxidase).", "content": "The original molecular weight determinations and hydrodynamic data on ceruloplasmin have later been contradicted by crystallographic and other investigations. In order to arrive at the correct copper stoichiometry of the protein we have undertaken a careful reinvestigation of some aspects of the physical chemistry and chemistry of ceruloplasmin with particular attention directed towards molecular weight and copper content. Human ceruloplasmin form I (the major form) was isoalted from fresh normal or retroplacental serum by a procedure designed to avoid proteolysis. The molecular weight of the protein, determined by meniscus depletion sedimentation equilibrium, was found to be 134 000+/-3 000. The sedimentation coefficient (7.25 S) and diffusion coefficient (4.46 10(-7) cm2/s) are consistent with this molecular weight. The frictional ratio calculated from these data, 1.41, and the observed intrinsic viscosity of 4.5 ml/g indicate that ceruloplasmin has a slightly more extended shape than a typical globular protein. This might at least partly be ascribed to its carbohydrate moiety. The amino acid composition, carbohydrate composition, and copper content of ceruloplasmin were determined. The copper content of several preparations varied between 6.0 and 6.6 atoms per mol. The copper stoichiometry of ceruloplasmin is discussed.", "contents": "Reinvestigation of some physicochemical and chemical properties of human ceruloplasmin (ferroxidase). The original molecular weight determinations and hydrodynamic data on ceruloplasmin have later been contradicted by crystallographic and other investigations. In order to arrive at the correct copper stoichiometry of the protein we have undertaken a careful reinvestigation of some aspects of the physical chemistry and chemistry of ceruloplasmin with particular attention directed towards molecular weight and copper content. Human ceruloplasmin form I (the major form) was isoalted from fresh normal or retroplacental serum by a procedure designed to avoid proteolysis. The molecular weight of the protein, determined by meniscus depletion sedimentation equilibrium, was found to be 134 000+/-3 000. The sedimentation coefficient (7.25 S) and diffusion coefficient (4.46 10(-7) cm2/s) are consistent with this molecular weight. The frictional ratio calculated from these data, 1.41, and the observed intrinsic viscosity of 4.5 ml/g indicate that ceruloplasmin has a slightly more extended shape than a typical globular protein. This might at least partly be ascribed to its carbohydrate moiety. The amino acid composition, carbohydrate composition, and copper content of ceruloplasmin were determined. The copper content of several preparations varied between 6.0 and 6.6 atoms per mol. The copper stoichiometry of ceruloplasmin is discussed.", "PMID": 952866}
{"id": "pubmed23n0032_260", "title": "Protein kinase associated with tubulin: affinity chromatography and properties.", "content": "Rat brain tubulin purified by colchicine-agarose affinity chromatography contains protein kinase activity. The kinase activity can be separated completely from tubulin by chromatography on casein columns and is not subsequently retained by colchicine affinity columns. Protein kinase activity associated with purified tubulin does not correlate with the total content of protein kinase activity in brain homogenates, since microtubules isolated from 48 000g fetal brain supernatants contain twice as much protein kinase activity than adult microtubules, although the total protein kinase activity is twice as high in the 48 000g adult supernatant. The protein kinase of tubulin preparations, while corresponding to a different molecule than tubulin, is probably not simply the result of contamination. These observations are interpreted in terms of specific associations between protein kinase and tubulin complexes. The protein kinase-tubulin association may be an important determinant in the regulation of tubulin function. Fetal tubulin polymerizes twice as well as adult tubulin in the absence of glycerol at the same tubulin concentration. The preferred substrate for the protein kinase either in vivo or in vitro (pH 7.4, 37 degrees C) is a specific high-molecular-weight protein, distinct from tubulin, which copurifies with tubulin through different kinds of isolation procedures (i.e., colchicine affinity chromatography and ammonium sulfate precipitation followed by diethylaminoethyl-cellulose chromatography). The tubulin-associated protein kinase is completely dependent on cyclic adenosine monophosphate (Km=10(-7)M), as demonstrated by the complete suppression of activity upon addition of the protein kinase modulator, a well-known specific inhibitor of cAMP-dependent protein kinases", "contents": "Protein kinase associated with tubulin: affinity chromatography and properties. Rat brain tubulin purified by colchicine-agarose affinity chromatography contains protein kinase activity. The kinase activity can be separated completely from tubulin by chromatography on casein columns and is not subsequently retained by colchicine affinity columns. Protein kinase activity associated with purified tubulin does not correlate with the total content of protein kinase activity in brain homogenates, since microtubules isolated from 48 000g fetal brain supernatants contain twice as much protein kinase activity than adult microtubules, although the total protein kinase activity is twice as high in the 48 000g adult supernatant. The protein kinase of tubulin preparations, while corresponding to a different molecule than tubulin, is probably not simply the result of contamination. These observations are interpreted in terms of specific associations between protein kinase and tubulin complexes. The protein kinase-tubulin association may be an important determinant in the regulation of tubulin function. Fetal tubulin polymerizes twice as well as adult tubulin in the absence of glycerol at the same tubulin concentration. The preferred substrate for the protein kinase either in vivo or in vitro (pH 7.4, 37 degrees C) is a specific high-molecular-weight protein, distinct from tubulin, which copurifies with tubulin through different kinds of isolation procedures (i.e., colchicine affinity chromatography and ammonium sulfate precipitation followed by diethylaminoethyl-cellulose chromatography). The tubulin-associated protein kinase is completely dependent on cyclic adenosine monophosphate (Km=10(-7)M), as demonstrated by the complete suppression of activity upon addition of the protein kinase modulator, a well-known specific inhibitor of cAMP-dependent protein kinases", "PMID": 952867}
{"id": "pubmed23n0032_261", "title": "Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate.", "content": "(Ethylenedinitrilo)tetraacetic acid soluble phosphoproteins were isolated from rat incisor and bovine unerupted teeth. This material was examined for its effect on the stability of amorphous calcium phosphate in vitro. When the precipitation of amorphous calcium phosphate was attempted in the presence of small amounts of these phospho-proteins, an apatite-like mineral was observed to form, which was approximately 60% crystalline, as determined by infrared measurements. This apatite phase could not be induced by addition of phosphoprotein after the precipitation reaction. The organic phosphate bound to these phosphoproteins was shown to be directly responsible for the formation of the apatite phase, since removal of 60% of the covalently bound phosphate with alkaline phosphatase destroyed the protein's ability to induce hydroxylapatite formation. The properties of the dental phosphoproteins appear to be consistent with their possible involvement in the development of the mineral phase of dentine.", "contents": "Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate. (Ethylenedinitrilo)tetraacetic acid soluble phosphoproteins were isolated from rat incisor and bovine unerupted teeth. This material was examined for its effect on the stability of amorphous calcium phosphate in vitro. When the precipitation of amorphous calcium phosphate was attempted in the presence of small amounts of these phospho-proteins, an apatite-like mineral was observed to form, which was approximately 60% crystalline, as determined by infrared measurements. This apatite phase could not be induced by addition of phosphoprotein after the precipitation reaction. The organic phosphate bound to these phosphoproteins was shown to be directly responsible for the formation of the apatite phase, since removal of 60% of the covalently bound phosphate with alkaline phosphatase destroyed the protein's ability to induce hydroxylapatite formation. The properties of the dental phosphoproteins appear to be consistent with their possible involvement in the development of the mineral phase of dentine.", "PMID": 952868}
{"id": "pubmed23n0032_262", "title": "Evidence against an acyl-enzyme intermediate in the reaction catalyzed by clostridial phosphotransacetylase.", "content": "Clostridial phosphotransacetylase catalyzes acyl group transfer between coenzyme A (CoA) and inorganic phosphate and also the arsenolysis of acetyl-coenzyme A (AcCoA) to yield acetate and CoA-SH. The enzyme mobility on sodium dodecyl sulfate electrophoresis corresponds to a molecular weight of 70 000. Kinetics of both forward and reverse reactions are of the ternary type as previously reported and product inhibition data are consistent with a random binding scheme. One essential sulfhydryl group per 70 000 daltons was inactivated in a pseudo-first-order process by either N-ethylmaleimide or 5,5'-dithiobis (nitrobenzoic acid). Reduction of the rate of this inactivation by 50% in the presence of AcCoA or acetyl phosphate concentrations near their kinetic K values demonstrates binding of these acyl donors in simple enzyme-substrate complexes. Moreover, pulse-chase experiments show these binary complexes to be functional and also show that they do not dissociate rapidly compared with their rates of catalytic turnover. Incubation of the enzyme with 14C-labeled acyl donors failed to produce labeled protein after passage through Sephadex. This was true despite efforts to mimic \"substrate synergism\" with desulfo-CoA or to compensate for unfavorable equilibria by means of CoA traps. Very slow isotope exchange reactions of 32Pi into acetyl phosphate and [3H]CoA into AcCoA were at first observed. As in the cases of several other enzymes recently reexamined, these were shown on careful inspection to be artifacts of contamination by second substrates. Attempts to detect exchange reactions between acetyl phosphate and Pi, even in the presence of the CoA analogue, desulfo-CoA, were also unsuccessful. Therefore, no evidence for an acyl-enzyme could be detected. Furthermore, our data allow us to develop arguments which, we believe, indicate that an acyl-enzyme intermediate is extremely improbable in the reaction catalyzed by phosphotransacetylase.", "contents": "Evidence against an acyl-enzyme intermediate in the reaction catalyzed by clostridial phosphotransacetylase. Clostridial phosphotransacetylase catalyzes acyl group transfer between coenzyme A (CoA) and inorganic phosphate and also the arsenolysis of acetyl-coenzyme A (AcCoA) to yield acetate and CoA-SH. The enzyme mobility on sodium dodecyl sulfate electrophoresis corresponds to a molecular weight of 70 000. Kinetics of both forward and reverse reactions are of the ternary type as previously reported and product inhibition data are consistent with a random binding scheme. One essential sulfhydryl group per 70 000 daltons was inactivated in a pseudo-first-order process by either N-ethylmaleimide or 5,5'-dithiobis (nitrobenzoic acid). Reduction of the rate of this inactivation by 50% in the presence of AcCoA or acetyl phosphate concentrations near their kinetic K values demonstrates binding of these acyl donors in simple enzyme-substrate complexes. Moreover, pulse-chase experiments show these binary complexes to be functional and also show that they do not dissociate rapidly compared with their rates of catalytic turnover. Incubation of the enzyme with 14C-labeled acyl donors failed to produce labeled protein after passage through Sephadex. This was true despite efforts to mimic \"substrate synergism\" with desulfo-CoA or to compensate for unfavorable equilibria by means of CoA traps. Very slow isotope exchange reactions of 32Pi into acetyl phosphate and [3H]CoA into AcCoA were at first observed. As in the cases of several other enzymes recently reexamined, these were shown on careful inspection to be artifacts of contamination by second substrates. Attempts to detect exchange reactions between acetyl phosphate and Pi, even in the presence of the CoA analogue, desulfo-CoA, were also unsuccessful. Therefore, no evidence for an acyl-enzyme could be detected. Furthermore, our data allow us to develop arguments which, we believe, indicate that an acyl-enzyme intermediate is extremely improbable in the reaction catalyzed by phosphotransacetylase.", "PMID": 952869}
{"id": "pubmed23n0032_263", "title": "Identification of the amatoxin-binding subunit of RNA polymerase B by affinity labeling experiments. Subunit B3-the true amatoxin receptor protein of multiple RNA polymerase B.", "content": "Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purification procedures for the [3H]A-P complex were based on radioactive assays. Phosphocellulose chromatography separated two radioactive components: PCI, the previously reported amatoxin binding protein, ABP (Brodner and Wieland, 1976), and PC II, the [3H]A-P complex. Sodium dodecyl sulfate gel electrophoresis of the complex showed the presence of a new heavy band very close to subunit B 1 and a decreased intensity of subunit band B 3. These were the only differences noted in the subunit structure of RNA polymerase B. [3H]Amanin was covalently coupled to the enzyme, affinity labeling, by a water-soluble carbodiimide and the resultant conjugate submitted to sodium dodecyl sulfate gel electrophoresis. The profile of radioactivity showed one main peak (greater than 2000 cpm) coinciding with the 550-nm absorption peak of subunit B 3 on a stained parallel gel. Since no other protein band contains any significant radioactivity, the binding site for [3H]amanin and most probably for all amatoxins is localized on the B 3 subunit SB 3.", "contents": "Identification of the amatoxin-binding subunit of RNA polymerase B by affinity labeling experiments. Subunit B3-the true amatoxin receptor protein of multiple RNA polymerase B. Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purification procedures for the [3H]A-P complex were based on radioactive assays. Phosphocellulose chromatography separated two radioactive components: PCI, the previously reported amatoxin binding protein, ABP (Brodner and Wieland, 1976), and PC II, the [3H]A-P complex. Sodium dodecyl sulfate gel electrophoresis of the complex showed the presence of a new heavy band very close to subunit B 1 and a decreased intensity of subunit band B 3. These were the only differences noted in the subunit structure of RNA polymerase B. [3H]Amanin was covalently coupled to the enzyme, affinity labeling, by a water-soluble carbodiimide and the resultant conjugate submitted to sodium dodecyl sulfate gel electrophoresis. The profile of radioactivity showed one main peak (greater than 2000 cpm) coinciding with the 550-nm absorption peak of subunit B 3 on a stained parallel gel. Since no other protein band contains any significant radioactivity, the binding site for [3H]amanin and most probably for all amatoxins is localized on the B 3 subunit SB 3.", "PMID": 952870}
{"id": "pubmed23n0032_264", "title": "Chemical characterization and subunit structure of human N-acetylhexosaminidases A and B.", "content": "Human hexosaminidases A and B were purified from placentae, using two stages of affinity chromatography, to a high degree of purity. Each enzyme was purified 5000-6000-fold, and isolated in 25-40% yield. Enzyme preparations appeared homogeneous in the analytical ultracentrifuge and by acrylamide gel electrophoresis. Hexosaminidase A contained 1.65 residues of sialic acid per molecule, whereas no sialic acid was present in hexosaminidase B. The molecular weights of the A and B isozymes as determined by gel filtration and sedimentation equilibrium are 100 000 and 108 000, respectively. In 5 M guanidine-HCl each of the enzymes yielded a 50 000-dalton species, which can further be dissociated into 25 000-dalton polypeptide chains by reduction and alkylation. The hexosaminidase B yielded one type of polypeptide chain, denoted beta, whereas the product from hexosaminidase A could be separated by ion-exchange chromatography into two species of chains, denoted alpha and beta, in equal amounts. The amino acid compositions of the separated alpha and beta chains were determined, and were found to correlate well with those of the intact enzymes. These findings enable the construction of a plausible model for the molecular structure of both enzymes. According to this model hexosaminidase A is composed of two subunits alpha2 and beta2, in which the two polypeptide chains are linked by a disulfide bridge. The structure of hexosaminidase B is, in parallel, beta2beta2. The suggested model is discussed in view of the accumulated information about the interrelationships between hexosaminidase A and B and the genetic metabolic disorders with which they are involved.", "contents": "Chemical characterization and subunit structure of human N-acetylhexosaminidases A and B. Human hexosaminidases A and B were purified from placentae, using two stages of affinity chromatography, to a high degree of purity. Each enzyme was purified 5000-6000-fold, and isolated in 25-40% yield. Enzyme preparations appeared homogeneous in the analytical ultracentrifuge and by acrylamide gel electrophoresis. Hexosaminidase A contained 1.65 residues of sialic acid per molecule, whereas no sialic acid was present in hexosaminidase B. The molecular weights of the A and B isozymes as determined by gel filtration and sedimentation equilibrium are 100 000 and 108 000, respectively. In 5 M guanidine-HCl each of the enzymes yielded a 50 000-dalton species, which can further be dissociated into 25 000-dalton polypeptide chains by reduction and alkylation. The hexosaminidase B yielded one type of polypeptide chain, denoted beta, whereas the product from hexosaminidase A could be separated by ion-exchange chromatography into two species of chains, denoted alpha and beta, in equal amounts. The amino acid compositions of the separated alpha and beta chains were determined, and were found to correlate well with those of the intact enzymes. These findings enable the construction of a plausible model for the molecular structure of both enzymes. According to this model hexosaminidase A is composed of two subunits alpha2 and beta2, in which the two polypeptide chains are linked by a disulfide bridge. The structure of hexosaminidase B is, in parallel, beta2beta2. The suggested model is discussed in view of the accumulated information about the interrelationships between hexosaminidase A and B and the genetic metabolic disorders with which they are involved.", "PMID": 952871}
{"id": "pubmed23n0032_265", "title": "Synergistic activation of adenylate cyclase by guanylyl imidophosphate and epinephrine.", "content": "A kinetic analysis of the synergistic activation of turkey erythrocyte adenylate cyclase by 1-catecholamines and guanylyl imidodiphosphate (Gpp(NH)p) is described. We have found that the role of the catecholamine hormone is to facilitate the activation of the enzyme by the guanyl nucleotide according to the following mechanism: R-E+G=R-EG R-EG+H=HR-EG leads to HR-E''G where R is the receptor, E the enzyme, G the guanyl nucleotide effector, and H the hormone. The binding steps are fast and reversible but the conversion of the inactive enzyme E to its active stable form (E'') occurs with a rate constant of k=0.7 min-1. This step is essentially irreversible in the presence of high Gpp(NH)p concentrations. In the absence of beta-agonist (1-catecholamine) and at low free Mg2+ concentrations, the activation of the enzyme is insignificant. At high Mg2+ concentration the conversion of E to E'' occurs slowly in the absence of hormone, probably by another pathway. Thus, the presence of a guanyl nucleotide at the allosteric site is obligatory but not sufficient to induce the conversion of the inactive enzyme to its active form. The process of enzyme activation requires both Gpp(NH)p and hormone and under these conditions is essentially irreversible. The permanently active enzyme is stable in the absence of hormone and Gpp(NH)p and its high catalytic activity is stable for many hours. However, hormone and ATP induce a conversion of the high activity to the low activity form. Thus, it seems that both the process of enzyme activation by Gpp(NH)p and its reversal are hormone dependent. Both processes are blocked by the beta-blocker propranolol.", "contents": "Synergistic activation of adenylate cyclase by guanylyl imidophosphate and epinephrine. A kinetic analysis of the synergistic activation of turkey erythrocyte adenylate cyclase by 1-catecholamines and guanylyl imidodiphosphate (Gpp(NH)p) is described. We have found that the role of the catecholamine hormone is to facilitate the activation of the enzyme by the guanyl nucleotide according to the following mechanism: R-E+G=R-EG R-EG+H=HR-EG leads to HR-E''G where R is the receptor, E the enzyme, G the guanyl nucleotide effector, and H the hormone. The binding steps are fast and reversible but the conversion of the inactive enzyme E to its active stable form (E'') occurs with a rate constant of k=0.7 min-1. This step is essentially irreversible in the presence of high Gpp(NH)p concentrations. In the absence of beta-agonist (1-catecholamine) and at low free Mg2+ concentrations, the activation of the enzyme is insignificant. At high Mg2+ concentration the conversion of E to E'' occurs slowly in the absence of hormone, probably by another pathway. Thus, the presence of a guanyl nucleotide at the allosteric site is obligatory but not sufficient to induce the conversion of the inactive enzyme to its active form. The process of enzyme activation requires both Gpp(NH)p and hormone and under these conditions is essentially irreversible. The permanently active enzyme is stable in the absence of hormone and Gpp(NH)p and its high catalytic activity is stable for many hours. However, hormone and ATP induce a conversion of the high activity to the low activity form. Thus, it seems that both the process of enzyme activation by Gpp(NH)p and its reversal are hormone dependent. Both processes are blocked by the beta-blocker propranolol.", "PMID": 952872}
{"id": "pubmed23n0032_266", "title": "Affinity labeling of bovine colostrum galactosyltransferase with a uridine 5'-diphosphate derivative.", "content": "The dialdehyde produced by the periodate cleavage of the ribose moiety of uridine 5'-diphosphate (UDP) has been used as an affinity label for the UDP-galactose/UDP binding site of galactosyltransferase from bovine colostrum. This derivative causes progressive inactivation of galactosyltransferase at a rate dependent on its concentration, and under certain conditions is a competitive inhibitor with respect to UDP-galactose. The substrate UDP-galactose protects the enzyme from inactivation. The inactivation is also dependent on Mn2+ concentration in a range that implies that the binding of Mn2+ at site I is a prerequisite for the binding of the UDP derivative. The inactivation can be progressively reversed by nitrogenous bases, or stabilized by KBH4 reduction, which is consistent with the hypothesis that a Schiff base has formed with a lysine residue. Galactosyltransferase was inactivated with a [3H]UDP derivative and the predominant labeled peptide, from thermolysin digestion, isolated and characterized as: Ser-Gly-Lys-UDP.", "contents": "Affinity labeling of bovine colostrum galactosyltransferase with a uridine 5'-diphosphate derivative. The dialdehyde produced by the periodate cleavage of the ribose moiety of uridine 5'-diphosphate (UDP) has been used as an affinity label for the UDP-galactose/UDP binding site of galactosyltransferase from bovine colostrum. This derivative causes progressive inactivation of galactosyltransferase at a rate dependent on its concentration, and under certain conditions is a competitive inhibitor with respect to UDP-galactose. The substrate UDP-galactose protects the enzyme from inactivation. The inactivation is also dependent on Mn2+ concentration in a range that implies that the binding of Mn2+ at site I is a prerequisite for the binding of the UDP derivative. The inactivation can be progressively reversed by nitrogenous bases, or stabilized by KBH4 reduction, which is consistent with the hypothesis that a Schiff base has formed with a lysine residue. Galactosyltransferase was inactivated with a [3H]UDP derivative and the predominant labeled peptide, from thermolysin digestion, isolated and characterized as: Ser-Gly-Lys-UDP.", "PMID": 952873}
{"id": "pubmed23n0032_267", "title": "A class of chromatin particles associated with nonhistone proteins.", "content": "Unfixed nucleoproteins may be banded isopycnically in metrizamide (2(3-acetamido-5-N-methylacetamido-2,4,6-triiodobenzamido)-2-deoxy-D-glucose) according to the protein/nucleic acid ratio. Unsheared or lightly sheared chromatin bands sharply (p=1.2 g/ml); it has a protein/DNA ratio of 1.4. Chromatin sheared by sonication to approximately 350 base pairs of DNA contains two components with protein/nucleic acid ratios of approximately 1.3 (p=1,185 g/ml) and 2 (p=1.245 g/ml). When chromatin is digested exhaustively with staphylococcal nuclease, two density components are found, one with a protein/DNA ratio of 1.5 (p=1.21 g/ml), the other with a protein/DNA ratio of 2 (p=1.24 g/ml). In both instances the denser particle (p=1.24 g/ml) contains nearly all the nonhistone proteins, while both dense and light fractions contain histones in similar amounts. The base sequence complexity of DNA from the fractions is not distinguishable from that of total DNA and there is no evidence of any concentration of sequences complementary to polysomal polyadenylated RNA molecules.", "contents": "A class of chromatin particles associated with nonhistone proteins. Unfixed nucleoproteins may be banded isopycnically in metrizamide (2(3-acetamido-5-N-methylacetamido-2,4,6-triiodobenzamido)-2-deoxy-D-glucose) according to the protein/nucleic acid ratio. Unsheared or lightly sheared chromatin bands sharply (p=1.2 g/ml); it has a protein/DNA ratio of 1.4. Chromatin sheared by sonication to approximately 350 base pairs of DNA contains two components with protein/nucleic acid ratios of approximately 1.3 (p=1,185 g/ml) and 2 (p=1.245 g/ml). When chromatin is digested exhaustively with staphylococcal nuclease, two density components are found, one with a protein/DNA ratio of 1.5 (p=1.21 g/ml), the other with a protein/DNA ratio of 2 (p=1.24 g/ml). In both instances the denser particle (p=1.24 g/ml) contains nearly all the nonhistone proteins, while both dense and light fractions contain histones in similar amounts. The base sequence complexity of DNA from the fractions is not distinguishable from that of total DNA and there is no evidence of any concentration of sequences complementary to polysomal polyadenylated RNA molecules.", "PMID": 952874}
{"id": "pubmed23n0032_268", "title": "Characterization of the association of specific proteins with messenger ribonucleic acid.", "content": "Isolation of messenger ribonucleic acid (mRNA)-protein particles from cytosol or dissociated polyribosomes yielded complexes in which several proteins were consistently associated with mRNA. Some of the mRNA-associated proteins appeared to have a high affinity for mRNA since they remained complexed to mRNA during centrifugation in CsCl gradients. Quantitation of RNA and protein in polyribosomal mRNPs suggested that each molecule of mRNA bound a molecule of each of the two major proteins of 78 000 and 52 000 apparent molecular weights and/or one or more of several minor proteins found in mRNPs. Of the several mRNP proteins, only the protein of 78 000 apparent molecular weight appeared to form a stable complex with the polyriboadenylic acid [poly(A)]-tract of mRNA, suggesting that the remaining mRNA-associated proteins bind to other regions which may be common to many or all mRNAs. Binding of [3H]poly(A)-rich RNA to mRNP proteins was effectively inhibited by unlabeled poly(A)-rich RNA or the homopolymer polyriboguanylic acid [poly(G)], but not by poly(A) or other natural or synthetic mRNAs. The properties of non-poly(A)-dependent binding of mRNA by mRNP protein were similar to those of mRNA binding by the guanosine triphosphate dependent Met-tRNAfMet-binding protein.", "contents": "Characterization of the association of specific proteins with messenger ribonucleic acid. Isolation of messenger ribonucleic acid (mRNA)-protein particles from cytosol or dissociated polyribosomes yielded complexes in which several proteins were consistently associated with mRNA. Some of the mRNA-associated proteins appeared to have a high affinity for mRNA since they remained complexed to mRNA during centrifugation in CsCl gradients. Quantitation of RNA and protein in polyribosomal mRNPs suggested that each molecule of mRNA bound a molecule of each of the two major proteins of 78 000 and 52 000 apparent molecular weights and/or one or more of several minor proteins found in mRNPs. Of the several mRNP proteins, only the protein of 78 000 apparent molecular weight appeared to form a stable complex with the polyriboadenylic acid [poly(A)]-tract of mRNA, suggesting that the remaining mRNA-associated proteins bind to other regions which may be common to many or all mRNAs. Binding of [3H]poly(A)-rich RNA to mRNP proteins was effectively inhibited by unlabeled poly(A)-rich RNA or the homopolymer polyriboguanylic acid [poly(G)], but not by poly(A) or other natural or synthetic mRNAs. The properties of non-poly(A)-dependent binding of mRNA by mRNP protein were similar to those of mRNA binding by the guanosine triphosphate dependent Met-tRNAfMet-binding protein.", "PMID": 952875}
{"id": "pubmed23n0032_269", "title": "In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose.", "content": "By means of affinity chromatography on poly(adenylic acid) (poly(A))-fixed Sepharose, protein fractions having strong affinity to poly(A) were prepared from postribosomal supernatants of rabbit reticulocyte and rat liver. These fractions contained several proteins similar by electrophoretic analysis to rabbit globin messenger ribonucleoprotein. Protein fractions from both sources were shown to form ribonucleoprotein complexes with rabbit globin mRNA, and these complexes sedimented at the same rate as native globin messenger ribonucleoprotein. Binding of the proteins to RNA was not highly specific, since not only poly(A) but also other polynucleotides as poly(C) or poly(U) were bound to these proteins. Ribosomal RNAs, tRNA, or DNAs did not bind the proteins. In order to ascertain the function of the poly(A)-Sepharose purified proteins, their effects on translation of globin mRNA was studied in vitro. Addition of rabbit reticulocyte protein to globin mRNA resulted in no more than a slight stimulation of both alpha- and beta-chain synthesis. Poly(A)-Sepharose purified protein from rat liver, however, caused a marked preferential reduction of alpha-chain synthesis. These results showed that at least some proteins in the poly(A)-Sepharose purified proteins affect the translation of globin. This inference suggested a possibility that protein moiety in globin mRNP might be involved in control of globin synthesis.", "contents": "In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose. By means of affinity chromatography on poly(adenylic acid) (poly(A))-fixed Sepharose, protein fractions having strong affinity to poly(A) were prepared from postribosomal supernatants of rabbit reticulocyte and rat liver. These fractions contained several proteins similar by electrophoretic analysis to rabbit globin messenger ribonucleoprotein. Protein fractions from both sources were shown to form ribonucleoprotein complexes with rabbit globin mRNA, and these complexes sedimented at the same rate as native globin messenger ribonucleoprotein. Binding of the proteins to RNA was not highly specific, since not only poly(A) but also other polynucleotides as poly(C) or poly(U) were bound to these proteins. Ribosomal RNAs, tRNA, or DNAs did not bind the proteins. In order to ascertain the function of the poly(A)-Sepharose purified proteins, their effects on translation of globin mRNA was studied in vitro. Addition of rabbit reticulocyte protein to globin mRNA resulted in no more than a slight stimulation of both alpha- and beta-chain synthesis. Poly(A)-Sepharose purified protein from rat liver, however, caused a marked preferential reduction of alpha-chain synthesis. These results showed that at least some proteins in the poly(A)-Sepharose purified proteins affect the translation of globin. This inference suggested a possibility that protein moiety in globin mRNP might be involved in control of globin synthesis.", "PMID": 952876}
{"id": "pubmed23n0032_270", "title": "The effect of various treatments in vitro and in vivo on the binding of 125I-labeled anti-rat serum albumin Fab' to rat tissue polyribosomes.", "content": "A quantitative assay was developed to permit estimation of the relative amounts of albumin-synthesizing polyribosomes in rat liver. The polyribosomes synthesizing albumin were identified by their capacity to bind anti-RSA Fab' radiolabeled with 125I. The anti-RSA Fab'-binding sites occur on the nascent peptide chains attached to liver polyribosomes. These binding sites can be saturated by preincubation of the polyribosomes with large quantities of unlabeled anti-RSA Fab'. The iodinated antibody did not react with polyribosomes isolated from a tissue which does not synthesize rat serum albumin. Pretreatment of hepatic polyribosomes with bovine pancreatic ribonuclease resulted in a 42% enhancement of binding of anti-RSA Fab'. Pretreatment of these polyribosomes with detergents or various levels of Mg2+ did not significantly affect the specific binding of the iodinated antibody. Anti-RSA Fab' associated preferentially with membrane-bound polyribosomes when compared with free polyribosomes following their isolation from animals maintained either on a 90% or a 0% protein diet fed ad libitum. Binding of anti-RSA Fab' to each A260 unit of membrane-bound polyribosomes is from 2.4 to 27 times greater than to each A260 unit of free polyribosomes. However, each A260 unit of free polyribosomes was found to associate with 1.8 times more anti-RSA Fab' when compared with the \"loosely bound\" subclass of membrane-bound polyribosomes. Each A260 unit of the \"tightly bound\" subclass of membrane-bound polyribosomes reacted with 4.3 times as much antibody as compared with free polyribosomes. Polyribosomes isolated from the livers of rats sacrificed 6 h after treatment with actinomycin D showed a 42% reduction in their capacity to bind anti-RSA Fab'. Polyribosomes from rats sacrificed 2 h after treatment with actinomycin D showed no reduction in binding capacity. Free polyribosomes from three Morris hepatomas were capable of binding anti-RSA Fab' whereas the antibody would not associate with the membrane-bound polyribosomes of the same hepatomas. Thus the binding of 125I-labeled Fab' antibody molecules to polyribosomes is a useful technique for the subcellular localization of polyribosomes synthesizing specific proteins and for the estimation of the relative proportions of such polyribosomes.", "contents": "The effect of various treatments in vitro and in vivo on the binding of 125I-labeled anti-rat serum albumin Fab' to rat tissue polyribosomes. A quantitative assay was developed to permit estimation of the relative amounts of albumin-synthesizing polyribosomes in rat liver. The polyribosomes synthesizing albumin were identified by their capacity to bind anti-RSA Fab' radiolabeled with 125I. The anti-RSA Fab'-binding sites occur on the nascent peptide chains attached to liver polyribosomes. These binding sites can be saturated by preincubation of the polyribosomes with large quantities of unlabeled anti-RSA Fab'. The iodinated antibody did not react with polyribosomes isolated from a tissue which does not synthesize rat serum albumin. Pretreatment of hepatic polyribosomes with bovine pancreatic ribonuclease resulted in a 42% enhancement of binding of anti-RSA Fab'. Pretreatment of these polyribosomes with detergents or various levels of Mg2+ did not significantly affect the specific binding of the iodinated antibody. Anti-RSA Fab' associated preferentially with membrane-bound polyribosomes when compared with free polyribosomes following their isolation from animals maintained either on a 90% or a 0% protein diet fed ad libitum. Binding of anti-RSA Fab' to each A260 unit of membrane-bound polyribosomes is from 2.4 to 27 times greater than to each A260 unit of free polyribosomes. However, each A260 unit of free polyribosomes was found to associate with 1.8 times more anti-RSA Fab' when compared with the \"loosely bound\" subclass of membrane-bound polyribosomes. Each A260 unit of the \"tightly bound\" subclass of membrane-bound polyribosomes reacted with 4.3 times as much antibody as compared with free polyribosomes. Polyribosomes isolated from the livers of rats sacrificed 6 h after treatment with actinomycin D showed a 42% reduction in their capacity to bind anti-RSA Fab'. Polyribosomes from rats sacrificed 2 h after treatment with actinomycin D showed no reduction in binding capacity. Free polyribosomes from three Morris hepatomas were capable of binding anti-RSA Fab' whereas the antibody would not associate with the membrane-bound polyribosomes of the same hepatomas. Thus the binding of 125I-labeled Fab' antibody molecules to polyribosomes is a useful technique for the subcellular localization of polyribosomes synthesizing specific proteins and for the estimation of the relative proportions of such polyribosomes.", "PMID": 952877}
{"id": "pubmed23n0032_271", "title": "Hormonal and nutritional effects on the binding of 125I-labeled anti-serine dehydratase Fab' to rat tissue polysomes.", "content": "With 125I-labeled Fab' specific for rat liver serine dehydratase it has been possible to localize polyribosomes synthesizing the enzyme under several different environmental conditions. Evidence is presented to show that, following the administration of amino acids in vivo, the relative synthetic capabilities of free and membrane-bound polyribosomes synthesizing serine dehydratase vary with time. Early during the period of induction of the enzyme by administration of amino acids or by feeding a high protein diet the majority of the newly synthesized enzyme is derived from membrane-bound polyribosomes. Later in the induction process an increasing proportion of the enzyme is synthesized by the free polyribosomes. Subcellular localization studies clearly show that serine dehydratase is synthesized by both subclasses of hepatic membrane-bound polyribosomes, the loose and tight membrane-bound polyribosomes, as well as by the free polyribosomes. It was found that the membrane-bound polyribosomes are the preferential sites of synthesis of the majority of serine dehydratase molecules in the Morris hepatomas 5123C and 7800. It is concluded that the synthesis of the enzyme, serine dehydratase, in rat liver is not discretely compartmentalized in either class of free or membrane-bound polyribosomes. Rather, the relative proportions of the serine dehydratase synthesizing polyribosomes within these two classes of polyribosomes can vary depending on the metabolic and physiologic state of the liver cell.", "contents": "Hormonal and nutritional effects on the binding of 125I-labeled anti-serine dehydratase Fab' to rat tissue polysomes. With 125I-labeled Fab' specific for rat liver serine dehydratase it has been possible to localize polyribosomes synthesizing the enzyme under several different environmental conditions. Evidence is presented to show that, following the administration of amino acids in vivo, the relative synthetic capabilities of free and membrane-bound polyribosomes synthesizing serine dehydratase vary with time. Early during the period of induction of the enzyme by administration of amino acids or by feeding a high protein diet the majority of the newly synthesized enzyme is derived from membrane-bound polyribosomes. Later in the induction process an increasing proportion of the enzyme is synthesized by the free polyribosomes. Subcellular localization studies clearly show that serine dehydratase is synthesized by both subclasses of hepatic membrane-bound polyribosomes, the loose and tight membrane-bound polyribosomes, as well as by the free polyribosomes. It was found that the membrane-bound polyribosomes are the preferential sites of synthesis of the majority of serine dehydratase molecules in the Morris hepatomas 5123C and 7800. It is concluded that the synthesis of the enzyme, serine dehydratase, in rat liver is not discretely compartmentalized in either class of free or membrane-bound polyribosomes. Rather, the relative proportions of the serine dehydratase synthesizing polyribosomes within these two classes of polyribosomes can vary depending on the metabolic and physiologic state of the liver cell.", "PMID": 952878}
{"id": "pubmed23n0032_272", "title": "Preparation of inside-out vesicles of pig lymphocyte plasma membrane.", "content": "Between 30 and 50% of pig lymphocyte plasma membrane vesicles were not bound by concanavalin A (Con A)-Sepharose. Various results suggest that the Con A-unretarded fraction represents \"inside-out\" membrane vesicles. First, an alternative cell surface ligand, anti-lymphocytic serum, gave a similar fractionation to Con A. Second, lack of binding by Con A was not due to lack of carbohydrate or to masking of carbohydrate by extraneous protein, because the unfractionated membrane and the unretarded fraction had similar carbohydrate and polypeptide compositions. Third although the carbohydrate of the unretarded membrane vesicles was accessible to 125I-labelled Con A and to release by soluble trypsin, it was not accessible to ferritin-Con A or trypsin-Sepharose. Fourth, antisera against the external surface of the Con A-unretarded vesicles strongly agglutinated the unretarded membrane, but caused negligible agglutination of whole lymphocytes. When attached to Sepharose these antisera bound all of the Con A-unretarded fraction, but failed to bind the membrane that adhered to Con A-Sepharose.", "contents": "Preparation of inside-out vesicles of pig lymphocyte plasma membrane. Between 30 and 50% of pig lymphocyte plasma membrane vesicles were not bound by concanavalin A (Con A)-Sepharose. Various results suggest that the Con A-unretarded fraction represents \"inside-out\" membrane vesicles. First, an alternative cell surface ligand, anti-lymphocytic serum, gave a similar fractionation to Con A. Second, lack of binding by Con A was not due to lack of carbohydrate or to masking of carbohydrate by extraneous protein, because the unfractionated membrane and the unretarded fraction had similar carbohydrate and polypeptide compositions. Third although the carbohydrate of the unretarded membrane vesicles was accessible to 125I-labelled Con A and to release by soluble trypsin, it was not accessible to ferritin-Con A or trypsin-Sepharose. Fourth, antisera against the external surface of the Con A-unretarded vesicles strongly agglutinated the unretarded membrane, but caused negligible agglutination of whole lymphocytes. When attached to Sepharose these antisera bound all of the Con A-unretarded fraction, but failed to bind the membrane that adhered to Con A-Sepharose.", "PMID": 952879}
{"id": "pubmed23n0032_273", "title": "High sensitivity amino acid sequence determination. Application to proteins eluted from polyacrylamide gels.", "content": "An automatic solid-phase procedure is described for determining the amino-terminal amino acid sequence of very small quantities of proteins. The sample is covalently attached to an inert support so that mechanical and physical losses during sequencing are eliminated. High sensitivity is achieved by using an initial coupling with high specific activity phenyl [35S]isothiocyanate followed by a longer reaction with the unlabeled reagent. The radioactive phenylthiohydantoins are identified by autoradiography after two-dimensional thin-layer chromatography. Unlabeled phenylthiohydantoin-amino acids are added to each fraction to assist in the identification and to act as carriers, hence reducing absorptive and extractive losses of the small quantities of sample. The method may be used on proteins eluted from polyacrylamide gels containing sodium dodecyl sulfate without removal of the detergent. Sequences of up to 20 residues have been obtained on quantities of protein ranging from 2.5 to 70 pmol. Results from proteins of hitherto unknown sequence are included.", "contents": "High sensitivity amino acid sequence determination. Application to proteins eluted from polyacrylamide gels. An automatic solid-phase procedure is described for determining the amino-terminal amino acid sequence of very small quantities of proteins. The sample is covalently attached to an inert support so that mechanical and physical losses during sequencing are eliminated. High sensitivity is achieved by using an initial coupling with high specific activity phenyl [35S]isothiocyanate followed by a longer reaction with the unlabeled reagent. The radioactive phenylthiohydantoins are identified by autoradiography after two-dimensional thin-layer chromatography. Unlabeled phenylthiohydantoin-amino acids are added to each fraction to assist in the identification and to act as carriers, hence reducing absorptive and extractive losses of the small quantities of sample. The method may be used on proteins eluted from polyacrylamide gels containing sodium dodecyl sulfate without removal of the detergent. Sequences of up to 20 residues have been obtained on quantities of protein ranging from 2.5 to 70 pmol. Results from proteins of hitherto unknown sequence are included.", "PMID": 952880}
{"id": "pubmed23n0032_274", "title": "Conformational properties of dinucleoside monophosphates in solution: dipurines and dipyrimidines.", "content": "In order to obtain information about the conformational features in a polyribonucleotide at the nearest neighbor level, detailed nuclear magnetic resonance studies of the dinucleoside monophosphates ApA, ApG, GpA, UpU, CpC, UpC, and CpU were undertaken. Proton spectra were recorded at 100, 220, 270, or 300 MHz for D2O solutions, 0.01-0.03 M, pD 7.4 at 20+/-2 degrees C. Spectra of ApA, ApG, UpU, and UpC were also recorded in the temperature range of 70-90 degrees C. Unambiguous signal assignments of all proton resonances were made with the aid of selectively deuterated dimers. Complete, accurate sets of nuclear magnetic resonance (NMR) parameters were derived for each nucleotidyl unit by simulation-iteration methods. A complete set of chemical shift and coupling constant data was also obtained for all the constituent monomeric units at a concentration and ionization state comparable to that of the dimers. Conformational properties were evaluated quantitatively for most of the bonds in the dinucleoside monophosphates using procedures developed in earlier studies. All of the dimers have a flexible conformational framework in aqueous solution. While flexibility is allowed and alternate conformations are accessible, these molecules nevertheless attempt to achieve conformational identity by showing preferences--sometimes overwhelming preferences--for certain orientations. Thus the ribose rings exist as equilibrium mixtures of C2'-endo in equilibrium C3'-endo conformers with a bias for the C3'-endo pucker in most cases. The C4'-C5' bonds of both nucleotidyl units show significant preference (70-85%) for a gg conformation. Similarly, the dominant conformer (80-90%) about C5'-O5' is g'g'. Even though an unambiguous determination of the orientation about C3'-O3' cannot be made, there is suggestive evidence that the orientation of the 3' phosphate group is coupled to the ribose conformational equilibrium and it is likely that a 3Eg- in equilibrium 2Eg+ equilibrium exists with a bias for the 3Eg- coupled conformation in which the H3'-C3'-O3'-P dihedral angle is about 34-38 degrees. The individual nucleotidyl units in the dimers differ in several key ways from corresponding monomer conformations. Specifically, the ribose equilibrium C2'-endo in equilibrium C3'-endo shifts in favor of C3'-endo upon dimerization, the only exception being UpU. The C4'-C5' and C5'-O5' bonding network in the dimer forms a stable conformational unit and no correlation exists in the dimers between the conformational preference of this fragment and ribose conformer population. The temperature data for the dimers and dimerization data clearly indicate that the transition C2'-endo leads to C3'-endo is directly related to XCN changes brought about by dimerization and stacking...", "contents": "Conformational properties of dinucleoside monophosphates in solution: dipurines and dipyrimidines. In order to obtain information about the conformational features in a polyribonucleotide at the nearest neighbor level, detailed nuclear magnetic resonance studies of the dinucleoside monophosphates ApA, ApG, GpA, UpU, CpC, UpC, and CpU were undertaken. Proton spectra were recorded at 100, 220, 270, or 300 MHz for D2O solutions, 0.01-0.03 M, pD 7.4 at 20+/-2 degrees C. Spectra of ApA, ApG, UpU, and UpC were also recorded in the temperature range of 70-90 degrees C. Unambiguous signal assignments of all proton resonances were made with the aid of selectively deuterated dimers. Complete, accurate sets of nuclear magnetic resonance (NMR) parameters were derived for each nucleotidyl unit by simulation-iteration methods. A complete set of chemical shift and coupling constant data was also obtained for all the constituent monomeric units at a concentration and ionization state comparable to that of the dimers. Conformational properties were evaluated quantitatively for most of the bonds in the dinucleoside monophosphates using procedures developed in earlier studies. All of the dimers have a flexible conformational framework in aqueous solution. While flexibility is allowed and alternate conformations are accessible, these molecules nevertheless attempt to achieve conformational identity by showing preferences--sometimes overwhelming preferences--for certain orientations. Thus the ribose rings exist as equilibrium mixtures of C2'-endo in equilibrium C3'-endo conformers with a bias for the C3'-endo pucker in most cases. The C4'-C5' bonds of both nucleotidyl units show significant preference (70-85%) for a gg conformation. Similarly, the dominant conformer (80-90%) about C5'-O5' is g'g'. Even though an unambiguous determination of the orientation about C3'-O3' cannot be made, there is suggestive evidence that the orientation of the 3' phosphate group is coupled to the ribose conformational equilibrium and it is likely that a 3Eg- in equilibrium 2Eg+ equilibrium exists with a bias for the 3Eg- coupled conformation in which the H3'-C3'-O3'-P dihedral angle is about 34-38 degrees. The individual nucleotidyl units in the dimers differ in several key ways from corresponding monomer conformations. Specifically, the ribose equilibrium C2'-endo in equilibrium C3'-endo shifts in favor of C3'-endo upon dimerization, the only exception being UpU. The C4'-C5' and C5'-O5' bonding network in the dimer forms a stable conformational unit and no correlation exists in the dimers between the conformational preference of this fragment and ribose conformer population. The temperature data for the dimers and dimerization data clearly indicate that the transition C2'-endo leads to C3'-endo is directly related to XCN changes brought about by dimerization and stacking...", "PMID": 952881}
{"id": "pubmed23n0032_275", "title": "Effect of 1-beta-D-arabinofuranosylcytosine triphosphate on DNA synthesis in isolated HeLa cell nuclei.", "content": "1-beta-D-Arabinofuranosylcytosine 5'-triphosphate (ara-CTP) was found to be a competitive inhibitor of DNA synthesis in nuclei isolated from synchronized S-phase HeLa S3 cells. The inhibition was reversed by dCTP. The main inhibitory effect was to reduce the rate of elongation of the primary DNA pieces from ara-CTP-treated nuclei after a 60-s pulse was about 115-120 nucleotides vs. 215-220 in control nuclei. A reduction in the frequency of initiation of primary DNA pieces probably also occurred. A slight inhibition of the transfer of labeled material from the primary pieces into high-molecular weight DNA was noted and probably arose from the fact that the reduced rate of growth of the primary pieces caused the critical size for ligation to be reached later. Synchronized HeLa S3 cells in suspension were treated with 1-beta-D-arabinofuranosylcytosine for 2 h in S-phase to give a 99% inhibition of DNA synthesis prior to isolation of nuclei. Neither the rate nor the extent of [3H]TTP incorporation into DNA decreased when such nuclei were incubated in the absence of ara-CTP, but the rate of ligation of primary pieces was reduced.", "contents": "Effect of 1-beta-D-arabinofuranosylcytosine triphosphate on DNA synthesis in isolated HeLa cell nuclei. 1-beta-D-Arabinofuranosylcytosine 5'-triphosphate (ara-CTP) was found to be a competitive inhibitor of DNA synthesis in nuclei isolated from synchronized S-phase HeLa S3 cells. The inhibition was reversed by dCTP. The main inhibitory effect was to reduce the rate of elongation of the primary DNA pieces from ara-CTP-treated nuclei after a 60-s pulse was about 115-120 nucleotides vs. 215-220 in control nuclei. A reduction in the frequency of initiation of primary DNA pieces probably also occurred. A slight inhibition of the transfer of labeled material from the primary pieces into high-molecular weight DNA was noted and probably arose from the fact that the reduced rate of growth of the primary pieces caused the critical size for ligation to be reached later. Synchronized HeLa S3 cells in suspension were treated with 1-beta-D-arabinofuranosylcytosine for 2 h in S-phase to give a 99% inhibition of DNA synthesis prior to isolation of nuclei. Neither the rate nor the extent of [3H]TTP incorporation into DNA decreased when such nuclei were incubated in the absence of ara-CTP, but the rate of ligation of primary pieces was reduced.", "PMID": 952882}
{"id": "pubmed23n0032_276", "title": "Ligand interactions with the acetylcholine receptor from Torpedo californica. Extensions of the allosteric model for cooperativity to half-of-site activity.", "content": "The solubilized acetylcholine receptor from Torpedo californica showed positive cooperativity in acetylcholine binding with a dissociation constant of 1.2 X 10(-8) M. Blockade of acetylcholine binding by nicotine was competitive; blockade by d-tubocurarine appeared to result from an allosteric interaction that altered half of the acetylcholine binding sites to a lower affinity form; decamethonium blockade displayed properties of competitive and allosteric inhibition suggesting less specificity for decamethonium binding than seen with either nicotine or d-tubocurarine. The d-tubocurarine inhibition data were evaluated by several possible models involving either differential competitive inhibition or allosteric inhibiton. The data were best described by the allosteric model.", "contents": "Ligand interactions with the acetylcholine receptor from Torpedo californica. Extensions of the allosteric model for cooperativity to half-of-site activity. The solubilized acetylcholine receptor from Torpedo californica showed positive cooperativity in acetylcholine binding with a dissociation constant of 1.2 X 10(-8) M. Blockade of acetylcholine binding by nicotine was competitive; blockade by d-tubocurarine appeared to result from an allosteric interaction that altered half of the acetylcholine binding sites to a lower affinity form; decamethonium blockade displayed properties of competitive and allosteric inhibition suggesting less specificity for decamethonium binding than seen with either nicotine or d-tubocurarine. The d-tubocurarine inhibition data were evaluated by several possible models involving either differential competitive inhibition or allosteric inhibiton. The data were best described by the allosteric model.", "PMID": 952883}
{"id": "pubmed23n0032_277", "title": "Cholinergic sites in skeletal muscle. II. Interaction of an agonist and two antagonists with the acetylcholine site.", "content": "The equilibrium interactions of alpha-bungarotoxin, d-tubocurarine, and carbamylcholine with junctional and extrajunctional skeletal muscle acetylcholine receptors were examined. d-Tubocurarine is a competitive inhibitor of the bindings of alpha-bungarotoxin to the acetylcholine receptor. No substantive difference was observed in the association of d-tubocurarine with the junctional and extrajunctional receptors. In contrast, the carbamylcholine inhibition of toxin binding is not competitive. The data indicate that either the single set of alpha-bungarotoxin and d-tubocurarine bindings sites contains two subsets of carbamylcholine sites or that the carbamylcholine binds in a cooperative manner to a single set of sites. In addition, the affinity of carbamylcholine for extrajunctional receptors may be higher than the affinity for junctional receptors.", "contents": "Cholinergic sites in skeletal muscle. II. Interaction of an agonist and two antagonists with the acetylcholine site. The equilibrium interactions of alpha-bungarotoxin, d-tubocurarine, and carbamylcholine with junctional and extrajunctional skeletal muscle acetylcholine receptors were examined. d-Tubocurarine is a competitive inhibitor of the bindings of alpha-bungarotoxin to the acetylcholine receptor. No substantive difference was observed in the association of d-tubocurarine with the junctional and extrajunctional receptors. In contrast, the carbamylcholine inhibition of toxin binding is not competitive. The data indicate that either the single set of alpha-bungarotoxin and d-tubocurarine bindings sites contains two subsets of carbamylcholine sites or that the carbamylcholine binds in a cooperative manner to a single set of sites. In addition, the affinity of carbamylcholine for extrajunctional receptors may be higher than the affinity for junctional receptors.", "PMID": 952884}
{"id": "pubmed23n0032_278", "title": "Binding of chloromethyl ketone substrate analogues to crystalline papain.", "content": "Papain (EC 3.4.22.2) is a proteolytic enzyme, the three-dimensional structure of which has been determined by x-ray diffraction at 2.8 A resolution (Drenth, J., Jansonius, J.N., Koekoek, R., Swen, H. M., and Wothers, B.G. (1968), Nature (London) 218, 929-932). The active site is a groove on the molecular surface in which the essential sulfhydryl group of cysteine-25 is situated next to the imidazole ring of histidine-159. The main object of this study was to determine by the difference-Fourier technique the binding mode for the substrate in the groove in order to explain the substrate specificity of the enzyme (P2 should have a hydrophobic side chain (Berger and Schechter, 1970) and to contribute to an elucidation of the catalytic mechanism. To this end, three chloromethyl ketone substrate analogues were reacted with the enzyme by covalent attachment to the sulfur atom of cysteine-25. The products crystallized isomorphously with the parent structure that is not the native, active enzyme but a mixture of oxidized papain (probably papain-SO2-) and papain with an extra cysteine attached to cysteine-25. Although this made the interpretation of the difference electron density maps less easy, it provided us with a clear picture of the way in which the acyl part of the substrate binds in the active site groove. The carbonyl oxygen of the P1 residue is near two potential hydrogen-bond donating groups, the backbone NH of cysteine-25 and the NH2 of glutamine-19. Valine residues 133 and 157 are responsible for the preference of papain in its substrate splitting. By removing the methylene group that covalently attaches the inhibitor molecules to the sulfur atom of cysteine-25 we obtained acceptable models for the acyl-enzyme structure and for the tetrahedral intermediate. The carbonyl oxygen of the P1 residue, carrying a formal negative charge in the tetrahedral intermediate, is stabilized by formation of two hydrogen bonds with the backbone NH of cysteine-25 and the NH2 group of glutamine-19. This situation resembles that suggested for the proteolytic serine enzymes (Henderson, R., Wright, C. S., Hess, G. P., and Blow, D. M. (1971), Cold Spring Harbor Symp. Quant. Biol. 36, 63-70; Robertus, J. D., Kraut, J., Alden, R. A., and Birktoft, J. J. (1972b), Biochemistry 11, 4293-4303). The nitrogen atom of the scissile peptide bond was found close to the imidazole ring of histidine-159, suggesting a role for this ring in protonating the N atom of the leaving group (Lowe, 1970). This proton transfer would be facilitated by a 30 degrees rotation of the ring around the C beta-Cgamma bond from an in-plane position with the sulfur atom to an in-plane position with the N atom. The possibility of this rotation is derived from a difference electron-density map for fully oxidizied papain vs. the parent protein.", "contents": "Binding of chloromethyl ketone substrate analogues to crystalline papain. Papain (EC 3.4.22.2) is a proteolytic enzyme, the three-dimensional structure of which has been determined by x-ray diffraction at 2.8 A resolution (Drenth, J., Jansonius, J.N., Koekoek, R., Swen, H. M., and Wothers, B.G. (1968), Nature (London) 218, 929-932). The active site is a groove on the molecular surface in which the essential sulfhydryl group of cysteine-25 is situated next to the imidazole ring of histidine-159. The main object of this study was to determine by the difference-Fourier technique the binding mode for the substrate in the groove in order to explain the substrate specificity of the enzyme (P2 should have a hydrophobic side chain (Berger and Schechter, 1970) and to contribute to an elucidation of the catalytic mechanism. To this end, three chloromethyl ketone substrate analogues were reacted with the enzyme by covalent attachment to the sulfur atom of cysteine-25. The products crystallized isomorphously with the parent structure that is not the native, active enzyme but a mixture of oxidized papain (probably papain-SO2-) and papain with an extra cysteine attached to cysteine-25. Although this made the interpretation of the difference electron density maps less easy, it provided us with a clear picture of the way in which the acyl part of the substrate binds in the active site groove. The carbonyl oxygen of the P1 residue is near two potential hydrogen-bond donating groups, the backbone NH of cysteine-25 and the NH2 of glutamine-19. Valine residues 133 and 157 are responsible for the preference of papain in its substrate splitting. By removing the methylene group that covalently attaches the inhibitor molecules to the sulfur atom of cysteine-25 we obtained acceptable models for the acyl-enzyme structure and for the tetrahedral intermediate. The carbonyl oxygen of the P1 residue, carrying a formal negative charge in the tetrahedral intermediate, is stabilized by formation of two hydrogen bonds with the backbone NH of cysteine-25 and the NH2 group of glutamine-19. This situation resembles that suggested for the proteolytic serine enzymes (Henderson, R., Wright, C. S., Hess, G. P., and Blow, D. M. (1971), Cold Spring Harbor Symp. Quant. Biol. 36, 63-70; Robertus, J. D., Kraut, J., Alden, R. A., and Birktoft, J. J. (1972b), Biochemistry 11, 4293-4303). The nitrogen atom of the scissile peptide bond was found close to the imidazole ring of histidine-159, suggesting a role for this ring in protonating the N atom of the leaving group (Lowe, 1970). This proton transfer would be facilitated by a 30 degrees rotation of the ring around the C beta-Cgamma bond from an in-plane position with the sulfur atom to an in-plane position with the N atom. The possibility of this rotation is derived from a difference electron-density map for fully oxidizied papain vs. the parent protein.", "PMID": 952885}
{"id": "pubmed23n0032_279", "title": "Studies on the intramolecular and intermolecular kinetic isotope effects in pyruvate carboxylase catalysis.", "content": "A deuterium kinetic isotope effect of 2.1 was observed when [2H3]pyruvate was used as the substrate for pyruvate carboxylase. The effect is on Vmax/Km alone and disappears at infinite substrate concentration. This is interpreted to mean that the slowest step in the overall catalysis is in the half-reaction involving the carboxylation of enzymebiotin by ATP and HCO3-. A tritium intramolecular isotope effect of 4.8 and an intermolecular effect of 1.2 were also observed. The former was interpreted as the isotope effect on the \"effective kcat\", while the latter the one on V max/Km. With these data, the rate constant for binding of pyruvate was estimated to be 4.5 X 10(6) M-1 min-1, and the deuterium kinetic isotope effect on the catalytic step to be 3.1. Relative values for various rate constants were also obtained. Fluoropyruvate was also shown to be a substrate, reacting six times slower. A deuterium kinetic isotope effect of 1.5 was observed, which remained even at infinite substrate concentration. This is interpreted to mean that the slowest step in the overall catalysis is now the carboxylation of fluoropyruvate.", "contents": "Studies on the intramolecular and intermolecular kinetic isotope effects in pyruvate carboxylase catalysis. A deuterium kinetic isotope effect of 2.1 was observed when [2H3]pyruvate was used as the substrate for pyruvate carboxylase. The effect is on Vmax/Km alone and disappears at infinite substrate concentration. This is interpreted to mean that the slowest step in the overall catalysis is in the half-reaction involving the carboxylation of enzymebiotin by ATP and HCO3-. A tritium intramolecular isotope effect of 4.8 and an intermolecular effect of 1.2 were also observed. The former was interpreted as the isotope effect on the \"effective kcat\", while the latter the one on V max/Km. With these data, the rate constant for binding of pyruvate was estimated to be 4.5 X 10(6) M-1 min-1, and the deuterium kinetic isotope effect on the catalytic step to be 3.1. Relative values for various rate constants were also obtained. Fluoropyruvate was also shown to be a substrate, reacting six times slower. A deuterium kinetic isotope effect of 1.5 was observed, which remained even at infinite substrate concentration. This is interpreted to mean that the slowest step in the overall catalysis is now the carboxylation of fluoropyruvate.", "PMID": 952886}
{"id": "pubmed23n0032_280", "title": "Poly(2-aminoadenylic acid): interaction with poly(uridylic acid).", "content": "Poly(2-aminoadenylic acid) forms both double and triple helices with poly(uridylic acid) [poly(U)]. The 2-amino group forms a third hydrogen bond, elevating the 2 leads to 1 transition temperature by 33 degrees C. The third strand, however, has about the same stability as poly(A)-2poly(U), as measured by Tm 3 leads to 2. This selective stabilization of the two-stranded helix results in a much greater resolution of the differnt thermal transitions than that observed in analogous polynucleotide systems. In contrast to other A, U systems 3 leads to 1 and 2 leads to 3 transitions are not observed under any conditions, and the triple helix always undergoes a 3 leads to 2 transition even at very high ionic strength. A 1:1 mixture of poly(2NH2A) and poly(U) exhibits no transient formation of 1:2 complex, unlike similar mixtures of poly(A) with poly(U) and poly(T). This difference is evidently due to a more rapid displacement reaction: [poly(2NH2A) + poly(2NH2A)-2poly(U) leads to 2 poly(2NH2A)-poly(U)] With poly(2NH2A) than with poly(A). We describe a method for establishing the combining ratios of polynucleotide complexes which used a computer to calculate the angles of intersection of mixing curves as explicit and continuous functions of the wavelength. The wavelength dispersions of the angles of intersection determine optimum wavelengths for establishing stoichiometry and can also provide reliable negative evidence that presumably plausible complexes are not formed. Analogous computer procedures have been developed to determine wavelengths which are selective for the formation of both 1:1 and 1:2 complexes. Infrared spectra of the 1:1 and 1:2 complexes resemble those of other A, U homoribopolynucleotide helices in having two and three strong bands, respectively, in the region of carbonyl stretching vibrations. CD spectra of the two complexes are unusual in having negative first extrema of moderate intensity. We attribute these extrema to intrastrand interactions of strong, well-resolved transitions at 278 nm (B2u) of the 2-aminoadenine residues. The CD spectra are correlated with those of other polynucleotide helices.", "contents": "Poly(2-aminoadenylic acid): interaction with poly(uridylic acid). Poly(2-aminoadenylic acid) forms both double and triple helices with poly(uridylic acid) [poly(U)]. The 2-amino group forms a third hydrogen bond, elevating the 2 leads to 1 transition temperature by 33 degrees C. The third strand, however, has about the same stability as poly(A)-2poly(U), as measured by Tm 3 leads to 2. This selective stabilization of the two-stranded helix results in a much greater resolution of the differnt thermal transitions than that observed in analogous polynucleotide systems. In contrast to other A, U systems 3 leads to 1 and 2 leads to 3 transitions are not observed under any conditions, and the triple helix always undergoes a 3 leads to 2 transition even at very high ionic strength. A 1:1 mixture of poly(2NH2A) and poly(U) exhibits no transient formation of 1:2 complex, unlike similar mixtures of poly(A) with poly(U) and poly(T). This difference is evidently due to a more rapid displacement reaction: [poly(2NH2A) + poly(2NH2A)-2poly(U) leads to 2 poly(2NH2A)-poly(U)] With poly(2NH2A) than with poly(A). We describe a method for establishing the combining ratios of polynucleotide complexes which used a computer to calculate the angles of intersection of mixing curves as explicit and continuous functions of the wavelength. The wavelength dispersions of the angles of intersection determine optimum wavelengths for establishing stoichiometry and can also provide reliable negative evidence that presumably plausible complexes are not formed. Analogous computer procedures have been developed to determine wavelengths which are selective for the formation of both 1:1 and 1:2 complexes. Infrared spectra of the 1:1 and 1:2 complexes resemble those of other A, U homoribopolynucleotide helices in having two and three strong bands, respectively, in the region of carbonyl stretching vibrations. CD spectra of the two complexes are unusual in having negative first extrema of moderate intensity. We attribute these extrema to intrastrand interactions of strong, well-resolved transitions at 278 nm (B2u) of the 2-aminoadenine residues. The CD spectra are correlated with those of other polynucleotide helices.", "PMID": 952887}
{"id": "pubmed23n0032_281", "title": "Temperature dependence of the 31P chemical shifts of nucleic acids. A prode of phosphate ester torsional conformations.", "content": "The temperature dependence of the 31P chemical shifts of the ribodinucleoside monophosphates, ApA, GpC, CpC, UpU, and ApU, of the deoxyribonucleic acids, d-ApT, TpT, d-ApA, and d-pApT, and of the homopolyribonucleic acids poly(G), poly(U), poly(A) is shown to provide information on the helix-coli transition in nucleic acids. The base stacked, helical structure with a gauche,gauche phosphate ester torsional conformation is 0.2-0.6 ppm upfield from the random coil conformation. In contrast, the 31P chemical shifts of dimethyl and diethyl phosphate do not change significantly with temperature. These results support our earlier hypothesis that 31P shifts are sensitive probes of torsional conformations with phosphate esters in gauche,gauche conformations having 31P shifts upfield from nongauche conformations.", "contents": "Temperature dependence of the 31P chemical shifts of nucleic acids. A prode of phosphate ester torsional conformations. The temperature dependence of the 31P chemical shifts of the ribodinucleoside monophosphates, ApA, GpC, CpC, UpU, and ApU, of the deoxyribonucleic acids, d-ApT, TpT, d-ApA, and d-pApT, and of the homopolyribonucleic acids poly(G), poly(U), poly(A) is shown to provide information on the helix-coli transition in nucleic acids. The base stacked, helical structure with a gauche,gauche phosphate ester torsional conformation is 0.2-0.6 ppm upfield from the random coil conformation. In contrast, the 31P chemical shifts of dimethyl and diethyl phosphate do not change significantly with temperature. These results support our earlier hypothesis that 31P shifts are sensitive probes of torsional conformations with phosphate esters in gauche,gauche conformations having 31P shifts upfield from nongauche conformations.", "PMID": 952888}
{"id": "pubmed23n0032_282", "title": "Isolation and purification of hen oviduct protein synthesis initiation factors A2A and A2B.", "content": "Two initiation factors, IF-A2A and IF-A2B, required for protein synthesis in a fractionated system have been isolated from hen oviduct. These factors were obtained from a 0.5 M KCl extraction of a nuclear-microsomal fraction of the oviduct. The crude extract inhibited protein synthesis, but, following DEAE-cellulose chromatography, activity was detected. Sephadex G-200 chromatography separated the activity into two active frations, A2A and A2B. These factors have been characterized with respect to their activities in polyphenylalanine polymerization at a low Mg2+ concentration, AUG- and GTP-dependent Met-tRNAf binding to 40S ribosomal subunits, the hydrolysis of GTP, and natural messenger ribonucleic acid (mRNA) dependent protein synthesis. In all of these systems A2A and A2B were able to substitute for rabbit reticulocyte M2A and M2B. The molecular weights of A2A and A2B have been estimated by gel filtration chromatography to be 280 000 and 23 000, respectively. Sedimentation analysis on sucrose gradients showed A2A to have a sedimentation coefficient of 5.2 S. Combining these data, the molecular weight of A2A was calculated to be 125 000. These values are similar to those for corresponding reticulocyte proteins. Finally, in the presence of added ovalbumin mRNA, A2A and A2B stimulated protein synthesis on non-salt-whashed hen oviduct and rabbit reticulocyte polysomes. Moreover, A2A- and A2B-dependent synthesis of ovalbumin was shown to occur on reticulocyte polysomes programmed with ovalbumin mRNA. This supports the conclusion that these factors are initiation factors for protein synthesis and not ribosomal structural proteins.", "contents": "Isolation and purification of hen oviduct protein synthesis initiation factors A2A and A2B. Two initiation factors, IF-A2A and IF-A2B, required for protein synthesis in a fractionated system have been isolated from hen oviduct. These factors were obtained from a 0.5 M KCl extraction of a nuclear-microsomal fraction of the oviduct. The crude extract inhibited protein synthesis, but, following DEAE-cellulose chromatography, activity was detected. Sephadex G-200 chromatography separated the activity into two active frations, A2A and A2B. These factors have been characterized with respect to their activities in polyphenylalanine polymerization at a low Mg2+ concentration, AUG- and GTP-dependent Met-tRNAf binding to 40S ribosomal subunits, the hydrolysis of GTP, and natural messenger ribonucleic acid (mRNA) dependent protein synthesis. In all of these systems A2A and A2B were able to substitute for rabbit reticulocyte M2A and M2B. The molecular weights of A2A and A2B have been estimated by gel filtration chromatography to be 280 000 and 23 000, respectively. Sedimentation analysis on sucrose gradients showed A2A to have a sedimentation coefficient of 5.2 S. Combining these data, the molecular weight of A2A was calculated to be 125 000. These values are similar to those for corresponding reticulocyte proteins. Finally, in the presence of added ovalbumin mRNA, A2A and A2B stimulated protein synthesis on non-salt-whashed hen oviduct and rabbit reticulocyte polysomes. Moreover, A2A- and A2B-dependent synthesis of ovalbumin was shown to occur on reticulocyte polysomes programmed with ovalbumin mRNA. This supports the conclusion that these factors are initiation factors for protein synthesis and not ribosomal structural proteins.", "PMID": 952889}
{"id": "pubmed23n0032_283", "title": "Digestion of RNA of chromatin and nuclear ribonucleoprotein by staphylococcal nuclease.", "content": "Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-labeled oligonucleotides were approximately 26 nucleotides in length. An RNA containing structure which sedimented no faster than 2 S could be isolated from the digests. Major and mino peptide species, of molecular weights 40 000 and 66 000, respectively, were associated with this structure isolated from either chromatin or nuclear ribonucleoprotein. The results demonstrate that some protein of nuclear ribonucleoprotein is complexed with the transcript while it is still associated with chromatin.", "contents": "Digestion of RNA of chromatin and nuclear ribonucleoprotein by staphylococcal nuclease. Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-labeled oligonucleotides were approximately 26 nucleotides in length. An RNA containing structure which sedimented no faster than 2 S could be isolated from the digests. Major and mino peptide species, of molecular weights 40 000 and 66 000, respectively, were associated with this structure isolated from either chromatin or nuclear ribonucleoprotein. The results demonstrate that some protein of nuclear ribonucleoprotein is complexed with the transcript while it is still associated with chromatin.", "PMID": 952890}
{"id": "pubmed23n0032_284", "title": "Use of gel chromatography for the determination of the Stokes radii of proteins in the presence and absence of detergents. A reexamination.", "content": "In the course of a routine investigation of the complex between the erythrocyte membrane protein spectrin and sodium dodecyl sulfate, we observed a large discrepancy between the true Stokes radius (178 A, measured by hydrodynamic methods)  and the apparent value derived from gel chromatography (107 A). In attempting to resolve this discrepancy, we have experiments that indicate that all large asymmetric particles may be subject to a similar discrepancy; e.g., native fibrinogne has a true Strokes radius of 108 A, whereas the value derived by column chromatography after calibration with globular proteins is only 71 A. The simplest interpretation is that end-on insertion of asymmetric particles into the gel pores contributes to their retardation. The phenomenon clearly limits the usefulness of gel chromatography as a quantitative measure of the hydrodynamic Stokes radius. Incidental data obtained in the course of this work indicate that spherical viruses may have weak chemical affinity for the porous gel. Chromatography of large proteins in the presence of detergents produced no effects ascribable to absorption of the detergents, but the results suggest a need for further  study of possible interaction between detergents and small gel pores.", "contents": "Use of gel chromatography for the determination of the Stokes radii of proteins in the presence and absence of detergents. A reexamination. In the course of a routine investigation of the complex between the erythrocyte membrane protein spectrin and sodium dodecyl sulfate, we observed a large discrepancy between the true Stokes radius (178 A, measured by hydrodynamic methods)  and the apparent value derived from gel chromatography (107 A). In attempting to resolve this discrepancy, we have experiments that indicate that all large asymmetric particles may be subject to a similar discrepancy; e.g., native fibrinogne has a true Strokes radius of 108 A, whereas the value derived by column chromatography after calibration with globular proteins is only 71 A. The simplest interpretation is that end-on insertion of asymmetric particles into the gel pores contributes to their retardation. The phenomenon clearly limits the usefulness of gel chromatography as a quantitative measure of the hydrodynamic Stokes radius. Incidental data obtained in the course of this work indicate that spherical viruses may have weak chemical affinity for the porous gel. Chromatography of large proteins in the presence of detergents produced no effects ascribable to absorption of the detergents, but the results suggest a need for further  study of possible interaction between detergents and small gel pores.", "PMID": 952891}
{"id": "pubmed23n0032_285", "title": "Bovine milk acid phosphatase. III. Purification and characterisation of the enzyme.", "content": "The acid phosphatase of bovine milk has been purified by ion-exchange chromatography on Amberlite CG-50 resin followed by Sephadex G-100 gel filtration and further purification steps using CM-Sephadex C-50, cellulose phosphate and affinity chromatography on alphas1-casein-Sepharose. These gave an apparently homogeneous enzyme with a specific activity of about 30 I.U./mg which possessed a molecular weight of about 42000 and E1%1cm = 9.4 at 280 nm. Electrophoresis under dissociating conditions, gel filtration in a 6.6 M urea buffer and ultracentrifugal analysis in the presence of 6 M guanidine HCl and 0.1 M 2-mercaptoethanol all gave molecular weight values close to 42000 indicating that the molecule was probably composed of a single polypeptide chain. The acid phosphatase was a glycoprotein with 2 mol/mol of galactose, 2 mol/mol of mannose and 4 mol/mol of glucosamine. Amino acid analysis showed that the enzyme was devoid of methionine.", "contents": "Bovine milk acid phosphatase. III. Purification and characterisation of the enzyme. The acid phosphatase of bovine milk has been purified by ion-exchange chromatography on Amberlite CG-50 resin followed by Sephadex G-100 gel filtration and further purification steps using CM-Sephadex C-50, cellulose phosphate and affinity chromatography on alphas1-casein-Sepharose. These gave an apparently homogeneous enzyme with a specific activity of about 30 I.U./mg which possessed a molecular weight of about 42000 and E1%1cm = 9.4 at 280 nm. Electrophoresis under dissociating conditions, gel filtration in a 6.6 M urea buffer and ultracentrifugal analysis in the presence of 6 M guanidine HCl and 0.1 M 2-mercaptoethanol all gave molecular weight values close to 42000 indicating that the molecule was probably composed of a single polypeptide chain. The acid phosphatase was a glycoprotein with 2 mol/mol of galactose, 2 mol/mol of mannose and 4 mol/mol of glucosamine. Amino acid analysis showed that the enzyme was devoid of methionine.", "PMID": 952892}
{"id": "pubmed23n0032_286", "title": "Experimental allergic aspermatogenic orchitis. IV. Chemical properties of sperm glycoproteins isolated from guinea pig testes.", "content": "Of four glycoproteins isolated from guinea pig testes, two were aspermatogenic (types I and IV) and two (types II and III) were inactive. The glycoproteins were rich in carbohydrate, varying from 41.5% to 49.5% carbohydrate by weight. Each glycoprotein had a unique amino acid composition, but in general low levels of tyrosine, tryptophan, and basic amino acids were found along with relatively high contents of serine, threonine, glutamic acid, and proline. Types I and IV glycoproteins were remarkably stable; their aspermatogenic activity was not affected by urea, trypsin, or heating at 100 degrees C in water or in 1 M HCl for 15 min. Carbohydrate analysis revealed little difference in the monosaccharide compositions of types I and IV glycoproteins, except that only the type I contained sialic acid. In contrast, types II and III glycoproteins lacked sialicacid and fucose and contained much less mannose. Both N-acetylglucosamine and N-acetylgalactosamine were present in all four glycoproteins, and they dominated in the types II and III. Fucose and at least 20-25% of the galactose appeared to occupy terminal positions in type IV glycoprotein as shown by their release after 15 min hydrolysis in 1 M HCl. All of the glycoproteins contained a relatively high percentage of galactose by weight, from 12.6 to 19.3%. The molecular weights of the glycoproteins were estimated by sodium dodecyl sulfate gel electrophoresis to be 47000, 105000 and 18000 respectively for the types I, II, and IV; type III glycoprotein showed two major bands, with molecular weights of 41500 and 22800. All the above molecular weight values are probably overestimated because of high carbohydrate content. The molecular weight of type IV glycoprotein was found to be 13000 by ultracentrifugation; a corrected value of 29000 was calculated for type I glycoprotein.", "contents": "Experimental allergic aspermatogenic orchitis. IV. Chemical properties of sperm glycoproteins isolated from guinea pig testes. Of four glycoproteins isolated from guinea pig testes, two were aspermatogenic (types I and IV) and two (types II and III) were inactive. The glycoproteins were rich in carbohydrate, varying from 41.5% to 49.5% carbohydrate by weight. Each glycoprotein had a unique amino acid composition, but in general low levels of tyrosine, tryptophan, and basic amino acids were found along with relatively high contents of serine, threonine, glutamic acid, and proline. Types I and IV glycoproteins were remarkably stable; their aspermatogenic activity was not affected by urea, trypsin, or heating at 100 degrees C in water or in 1 M HCl for 15 min. Carbohydrate analysis revealed little difference in the monosaccharide compositions of types I and IV glycoproteins, except that only the type I contained sialic acid. In contrast, types II and III glycoproteins lacked sialicacid and fucose and contained much less mannose. Both N-acetylglucosamine and N-acetylgalactosamine were present in all four glycoproteins, and they dominated in the types II and III. Fucose and at least 20-25% of the galactose appeared to occupy terminal positions in type IV glycoprotein as shown by their release after 15 min hydrolysis in 1 M HCl. All of the glycoproteins contained a relatively high percentage of galactose by weight, from 12.6 to 19.3%. The molecular weights of the glycoproteins were estimated by sodium dodecyl sulfate gel electrophoresis to be 47000, 105000 and 18000 respectively for the types I, II, and IV; type III glycoprotein showed two major bands, with molecular weights of 41500 and 22800. All the above molecular weight values are probably overestimated because of high carbohydrate content. The molecular weight of type IV glycoprotein was found to be 13000 by ultracentrifugation; a corrected value of 29000 was calculated for type I glycoprotein.", "PMID": 952893}
{"id": "pubmed23n0032_287", "title": "Comparative analysis of the nuclear basic proteins in rat, human, guinea pig, mouse and rabbit spermatozoa.", "content": "Cysteine-rich protamines (Arg = 47-61%, Cys = 8-16%) were isolated from the sperm of an individual guinea pig, human and rabbit and from pooled samples of mouse and rat sperm. Appreciable concentrations of histones were not found in the sperm nuclei of these species. In addition to the protamines, a substance of relatively low molecular weight, which reacted with the Lowry reagent, appeared in crude acid-soluble extracts of sperm nucleoprotein. This unidentified contaminent was resolved from the protamines by chromatography on Bio-Rex 70. Heterogeneity of human and mouse protamines was revealed by electrophoresis at pH 2.7, in the presence of 2.5 M urea, and confirmed by amino acid analysis, which also suggested the presence of 2 or more species of protamine in the rabbit. By contrast, the guinea pig and rat preparations displayed nearly stoichiometric ratios of amino acid residues, approaching homogeneity by this criterion. The functional consequences of crosslinks between cysteine residues of these proteins and the possible species-specific significance of their differing percentages of histidine are discussed. Potentially analogous functions are suggested for phosphorylated serine and threonine, and for ionized cysteine and tyrosine, within the protamines of developing spermatids. Their amino acid compositions indicate that the protamines of eutherian mammals are coded by a C.G-rich genome which has been unusually susceptible to genetic drift. An especially high rate of G leads to A transitions seems to have occurred in the human protamine genes.", "contents": "Comparative analysis of the nuclear basic proteins in rat, human, guinea pig, mouse and rabbit spermatozoa. Cysteine-rich protamines (Arg = 47-61%, Cys = 8-16%) were isolated from the sperm of an individual guinea pig, human and rabbit and from pooled samples of mouse and rat sperm. Appreciable concentrations of histones were not found in the sperm nuclei of these species. In addition to the protamines, a substance of relatively low molecular weight, which reacted with the Lowry reagent, appeared in crude acid-soluble extracts of sperm nucleoprotein. This unidentified contaminent was resolved from the protamines by chromatography on Bio-Rex 70. Heterogeneity of human and mouse protamines was revealed by electrophoresis at pH 2.7, in the presence of 2.5 M urea, and confirmed by amino acid analysis, which also suggested the presence of 2 or more species of protamine in the rabbit. By contrast, the guinea pig and rat preparations displayed nearly stoichiometric ratios of amino acid residues, approaching homogeneity by this criterion. The functional consequences of crosslinks between cysteine residues of these proteins and the possible species-specific significance of their differing percentages of histidine are discussed. Potentially analogous functions are suggested for phosphorylated serine and threonine, and for ionized cysteine and tyrosine, within the protamines of developing spermatids. Their amino acid compositions indicate that the protamines of eutherian mammals are coded by a C.G-rich genome which has been unusually susceptible to genetic drift. An especially high rate of G leads to A transitions seems to have occurred in the human protamine genes.", "PMID": 952894}
{"id": "pubmed23n0032_288", "title": "The effect of ligand size and stereochemistry on the reactivity of the alpha and beta chains within hemoglobin.", "content": "The reactions of human hemoglobin A with methyl, ethyl, n-propyl, n-butyl, iso-butyl, sec-butyl, and tert-butyl isocyanide were examined in the presence and absence of inositol hexaphosphate. As the size and bulk of the aliphatic side-chain increases, the relative association rates and affinities of the beta-chains for isonitriles increase compared to those of the alpha chains. This result indicates that the beta heme pocket within hemoglobin is more open and accessible to ligand molecules than the alpha heme pocket.", "contents": "The effect of ligand size and stereochemistry on the reactivity of the alpha and beta chains within hemoglobin. The reactions of human hemoglobin A with methyl, ethyl, n-propyl, n-butyl, iso-butyl, sec-butyl, and tert-butyl isocyanide were examined in the presence and absence of inositol hexaphosphate. As the size and bulk of the aliphatic side-chain increases, the relative association rates and affinities of the beta-chains for isonitriles increase compared to those of the alpha chains. This result indicates that the beta heme pocket within hemoglobin is more open and accessible to ligand molecules than the alpha heme pocket.", "PMID": 952895}
{"id": "pubmed23n0032_289", "title": "Dependence of magneto-optical rotatory dispersion and magnetic circular dichroism of deoxy- and methemoglobin on their quaternary structure.", "content": "Methods of magnetic optical activity, magneto-optical rotatory dispersion (MORD) and magnetic circular dichroism (MCD), were shown to be sensitivie to the quaternary structure of deoxyhemoglobin. The isolated alpha and beta chains, the monomeric hemoglobins (leghemoglobin, fraction III of Chironomus thummi thummi hemoglobin) and hemoglobin in the R state (hemoglobin digested with carboxypeptidases A and B) exhibit in the visible region two MORD minima of equal intensities. In native tetrameric hemoglobins studied (human, horse, porcine, feline, carp, tortoise, frog) the ratio of the intensities of these MORD minima is about 2. The MORD data for deoxy-des Arg-N-ethylsuccinimide-hemoglobin indicate that in solution there is a mixture of the T and R states, the equilibrium between the states being shifted toward the R structure. The identity of the MORD curves for deoxy-bis(N-maleimidoethyl)-ester-hemoglobin and for native deoxyhemoglobin indicates that deoxy-bis(N-maleimidoethyl)ester-hemoglobin has the T structure in solution. Comparison of the MORD curves exhibited by a native methemoglobin, a native metmyoglobin and the modified hemoglobins in the met form in the absence and presence of organic and inorganic phosphates reveals no direct correlation between the MORD changes and methemoglobin quaternary structure.", "contents": "Dependence of magneto-optical rotatory dispersion and magnetic circular dichroism of deoxy- and methemoglobin on their quaternary structure. Methods of magnetic optical activity, magneto-optical rotatory dispersion (MORD) and magnetic circular dichroism (MCD), were shown to be sensitivie to the quaternary structure of deoxyhemoglobin. The isolated alpha and beta chains, the monomeric hemoglobins (leghemoglobin, fraction III of Chironomus thummi thummi hemoglobin) and hemoglobin in the R state (hemoglobin digested with carboxypeptidases A and B) exhibit in the visible region two MORD minima of equal intensities. In native tetrameric hemoglobins studied (human, horse, porcine, feline, carp, tortoise, frog) the ratio of the intensities of these MORD minima is about 2. The MORD data for deoxy-des Arg-N-ethylsuccinimide-hemoglobin indicate that in solution there is a mixture of the T and R states, the equilibrium between the states being shifted toward the R structure. The identity of the MORD curves for deoxy-bis(N-maleimidoethyl)-ester-hemoglobin and for native deoxyhemoglobin indicates that deoxy-bis(N-maleimidoethyl)ester-hemoglobin has the T structure in solution. Comparison of the MORD curves exhibited by a native methemoglobin, a native metmyoglobin and the modified hemoglobins in the met form in the absence and presence of organic and inorganic phosphates reveals no direct correlation between the MORD changes and methemoglobin quaternary structure.", "PMID": 952896}
{"id": "pubmed23n0032_290", "title": "Biochemical studies on human ceruloplasmin.", "content": "Ceruloplasmin from nephrotic urine, ascites fluid and plasma has been partially characterized. All ceruloplasmin preparations were found to be comprised of two light and two heavy polypeptide subunits. Characterization of the purified subunits indicated that the alpha chain had a mol. wt. of 16000 and had N-terminal valine while the beta chain had a mol. wt. of 59000 and had N-terminal lysine. All carbohydrate resided in the beta subunit. Incomplete cleavage of the 5-methionine residues of the alpha chain enabled a preliminary ordering of the CNBr fragments. Automated sequence analysis of the alpha chain was carried out and the sequence determined was Val-Phe-Asx-Pro-Arg-Arg-Lys-Leu-Glx-Phe-Ala-Leu-Leu-Phe-Leu-Val-Phe-Asx-Glx-Asx-Glx.", "contents": "Biochemical studies on human ceruloplasmin. Ceruloplasmin from nephrotic urine, ascites fluid and plasma has been partially characterized. All ceruloplasmin preparations were found to be comprised of two light and two heavy polypeptide subunits. Characterization of the purified subunits indicated that the alpha chain had a mol. wt. of 16000 and had N-terminal valine while the beta chain had a mol. wt. of 59000 and had N-terminal lysine. All carbohydrate resided in the beta subunit. Incomplete cleavage of the 5-methionine residues of the alpha chain enabled a preliminary ordering of the CNBr fragments. Automated sequence analysis of the alpha chain was carried out and the sequence determined was Val-Phe-Asx-Pro-Arg-Arg-Lys-Leu-Glx-Phe-Ala-Leu-Leu-Phe-Leu-Val-Phe-Asx-Glx-Asx-Glx.", "PMID": 952897}
{"id": "pubmed23n0032_291", "title": "The heterogeneity of the protamines from human spermatozoa.", "content": "Nuclear basic protein from ejaculated human spermatozoa were labelled with iodo[14C1]acetic acid and fractionated by ion-exchange chromatography into several pools (named A-K). Gel electrophoresis indicated that the minor protamine components, were present in pool D and that, of the major protamine components, component 1 (pools E, F, G, H) was well separated from the unresolved mixture of component 2 and component 3 (pools I, J, K). Pools G and J were free of other contaminants. Pools D, G, and J produced different radioactive peptides on digestion with trypsin and with thermolysin, and also had quite distinct amino acid compositions. This suggests that the heterogeneity of human protamines is caused by differences in amino acid sequence. Major component 1 also seems to be heterogenous, since it was found in two distinct peaks (pools E and G), but post-translational modification as a cause of the two types of component 1 has not been ruled out. Although all the human protamine components are similar to other mammalian protamines in containing half-cysteine and tyrosine, they also have unique common features such as high histidine and high glutamic acid contents.", "contents": "The heterogeneity of the protamines from human spermatozoa. Nuclear basic protein from ejaculated human spermatozoa were labelled with iodo[14C1]acetic acid and fractionated by ion-exchange chromatography into several pools (named A-K). Gel electrophoresis indicated that the minor protamine components, were present in pool D and that, of the major protamine components, component 1 (pools E, F, G, H) was well separated from the unresolved mixture of component 2 and component 3 (pools I, J, K). Pools G and J were free of other contaminants. Pools D, G, and J produced different radioactive peptides on digestion with trypsin and with thermolysin, and also had quite distinct amino acid compositions. This suggests that the heterogeneity of human protamines is caused by differences in amino acid sequence. Major component 1 also seems to be heterogenous, since it was found in two distinct peaks (pools E and G), but post-translational modification as a cause of the two types of component 1 has not been ruled out. Although all the human protamine components are similar to other mammalian protamines in containing half-cysteine and tyrosine, they also have unique common features such as high histidine and high glutamic acid contents.", "PMID": 952898}
{"id": "pubmed23n0032_292", "title": "DNA-binding proteins in Yoshida ascites tumor fluid.", "content": "DNA-binding proteins were isolated from Yoshida ascites tumor fluid by chromatography on DNA-cellulose. This fraction represents 1-2% of the total ascites protein. Most of the DNA-binding proteins will bind to phosphocellulose as well. The proteins migrate by agarose gel electrophoresis at pH 8.6 as alpha and beta globulins. Quantitative immunoelectrophoresis revealed the presence of 12-18 proteins. SDS-polyacrylamide electrophoresis indicated molecular weights ranging from 3-10(4) to 10(6). Seven of the proteins were identified by specific immunoprecipitation as beta1-Eglobulin, beta2-glycoprotein I, fibrinogen split product E (fibrinogen E), coagulation factor XIII (factor XIII), alpha2-macroglobulin, IgG and IgM. Alpha1-antichymotrypsin might also be represented. In nuclear extracts of the tumor cells only factor XIII was present. With the exception of fibrinogen E and P5 all recognized DNA-binding proteins are present in normal rat plasma. With increasing tumor age the concentration of fibrinogen E, factor XIII, P5 and IgM increased both in ascites fluid and in plasma, while the concentration of other DNA-binding-proteins decreased or remained constant. Evidence is presented that the DNA- and phosphocellulose binding ascites protein fraction inhibit tumor cell growth. No inhibition was induced by corresponding protein fractions isolated from normal rat plasma.", "contents": "DNA-binding proteins in Yoshida ascites tumor fluid. DNA-binding proteins were isolated from Yoshida ascites tumor fluid by chromatography on DNA-cellulose. This fraction represents 1-2% of the total ascites protein. Most of the DNA-binding proteins will bind to phosphocellulose as well. The proteins migrate by agarose gel electrophoresis at pH 8.6 as alpha and beta globulins. Quantitative immunoelectrophoresis revealed the presence of 12-18 proteins. SDS-polyacrylamide electrophoresis indicated molecular weights ranging from 3-10(4) to 10(6). Seven of the proteins were identified by specific immunoprecipitation as beta1-Eglobulin, beta2-glycoprotein I, fibrinogen split product E (fibrinogen E), coagulation factor XIII (factor XIII), alpha2-macroglobulin, IgG and IgM. Alpha1-antichymotrypsin might also be represented. In nuclear extracts of the tumor cells only factor XIII was present. With the exception of fibrinogen E and P5 all recognized DNA-binding proteins are present in normal rat plasma. With increasing tumor age the concentration of fibrinogen E, factor XIII, P5 and IgM increased both in ascites fluid and in plasma, while the concentration of other DNA-binding-proteins decreased or remained constant. Evidence is presented that the DNA- and phosphocellulose binding ascites protein fraction inhibit tumor cell growth. No inhibition was induced by corresponding protein fractions isolated from normal rat plasma.", "PMID": 952899}
{"id": "pubmed23n0032_293", "title": "Effects of organic and inorganic phosphate ions on the charge-transfer band of ferric myoglobin.", "content": "(1) Splitting of the charge-transfer band positioned at 630 nm of metmyoglobin disappeared on lowering the pH from 7.5 to 4.5 in the presence of Pi and PPi at the temperature of liquid N2. (2) The observed apparent pK value of the disappearance of the splitting depended upon the species of phosphate ions. (3) In the case of Pi, the low-temperature absorption spectra suggested that at least two phosphate ions bound to one myoglobin molecule.", "contents": "Effects of organic and inorganic phosphate ions on the charge-transfer band of ferric myoglobin. (1) Splitting of the charge-transfer band positioned at 630 nm of metmyoglobin disappeared on lowering the pH from 7.5 to 4.5 in the presence of Pi and PPi at the temperature of liquid N2. (2) The observed apparent pK value of the disappearance of the splitting depended upon the species of phosphate ions. (3) In the case of Pi, the low-temperature absorption spectra suggested that at least two phosphate ions bound to one myoglobin molecule.", "PMID": 952900}
{"id": "pubmed23n0032_294", "title": "Activities of nucleoprotein particles derived from rat liver ribosome.", "content": "80-S ribosomes and 60-S subunits from rat liver were treated at increasing KC1 concentrations giving protein-deficient ribosomal particles whose components were analyzed and their activity tested. Most of the activities assayed stand treatment up to KC1 concentrations of around 0.6 M; peptidyl transferase, measured by the fragment reaction, however was 50% inhibited by 0.5 M KC1 in 60-S subunits but not in 80-S ribosomes. Three proteins, L21, L26 and L31, might be implicated in this loss of activity. 60-S subunits forming part of the 80 S ribosome are more resistant to the salt treatment and the pattern of proteins released by the treatment differs from the one obtained from free 60-S subunits, implying perhaps a change of conformation of this subunit upon association to form 80-S couples. According to their resistance to release by KC1 the proteins of the large sub-unit can be divided into three groups: (1) easily removed, including proteins: L1, L11, L17 and L25 in 80-s subunits and in addition, L5, L8, L9, L13, L20, L22, L26, L29, L31 and L32/33 in 60-S subunits; (2) proteins resistant to release by high salt concentrations in 80-S ribosomes as well as in 60-S subunits, namely proteins L3, L14, L27, L36, L40, L41, X1 and X2; (3) the rest of the proteins which are released in a more or less continuous way throughout the treatment. 5 S RNA is not released by KC1 treatment at the concentrations used. The binding sites for the antibiotics trichodermin and anisomycin are affected in a different way by the salt treatment, indicating that they are structurally different.", "contents": "Activities of nucleoprotein particles derived from rat liver ribosome. 80-S ribosomes and 60-S subunits from rat liver were treated at increasing KC1 concentrations giving protein-deficient ribosomal particles whose components were analyzed and their activity tested. Most of the activities assayed stand treatment up to KC1 concentrations of around 0.6 M; peptidyl transferase, measured by the fragment reaction, however was 50% inhibited by 0.5 M KC1 in 60-S subunits but not in 80-S ribosomes. Three proteins, L21, L26 and L31, might be implicated in this loss of activity. 60-S subunits forming part of the 80 S ribosome are more resistant to the salt treatment and the pattern of proteins released by the treatment differs from the one obtained from free 60-S subunits, implying perhaps a change of conformation of this subunit upon association to form 80-S couples. According to their resistance to release by KC1 the proteins of the large sub-unit can be divided into three groups: (1) easily removed, including proteins: L1, L11, L17 and L25 in 80-s subunits and in addition, L5, L8, L9, L13, L20, L22, L26, L29, L31 and L32/33 in 60-S subunits; (2) proteins resistant to release by high salt concentrations in 80-S ribosomes as well as in 60-S subunits, namely proteins L3, L14, L27, L36, L40, L41, X1 and X2; (3) the rest of the proteins which are released in a more or less continuous way throughout the treatment. 5 S RNA is not released by KC1 treatment at the concentrations used. The binding sites for the antibiotics trichodermin and anisomycin are affected in a different way by the salt treatment, indicating that they are structurally different.", "PMID": 952902}
{"id": "pubmed23n0032_295", "title": "Preformed poly(A)-containing RNA in zoospores of Blastocladiella emersonii.", "content": "Discharged zoospores of Blastocladiella emersonii contain poly(A)-containing RNA, which is performed during the initial stages of zoospore differentiation in the sporangium and is stable in vivo for at least 4 h. The average molecular weight of this RNA fraction is estimation to be 5.5-10(5) or 1600 nucleotides. The average length of the poly(A) sequence is approx. 100 nucleotides Poly(A)-containing RNA does not appear to accumulate in a gamma-particle fraction previously suggested to be involved in the storage of mRNA.", "contents": "Preformed poly(A)-containing RNA in zoospores of Blastocladiella emersonii. Discharged zoospores of Blastocladiella emersonii contain poly(A)-containing RNA, which is performed during the initial stages of zoospore differentiation in the sporangium and is stable in vivo for at least 4 h. The average molecular weight of this RNA fraction is estimation to be 5.5-10(5) or 1600 nucleotides. The average length of the poly(A) sequence is approx. 100 nucleotides Poly(A)-containing RNA does not appear to accumulate in a gamma-particle fraction previously suggested to be involved in the storage of mRNA.", "PMID": 952903}
{"id": "pubmed23n0032_296", "title": "Effects of estrogen and progesterone on transcription, chromatin and ovalbumin gene expression in the chick oviduct.", "content": "The effects of estrogen, progesterone and estrogen + progesterone combined on nuclear transcriptional processes in oviducts of immature chicks, previously withdrawn from estrogen, are reported. The responses to the steroids of the endogenous nuclear RNA polymerase activities, both nucleolar (I) and nucleoplasmic (II), the chromatin compositions and template capacities, and the appearance of ovalbumin messenger RNA (mRNA) are compared. When immature chicks (previously treated at 14 days with estrogen) are withdrawn from estrogen treatment, there is a gradual reduction in both polymerase activities. Diurnal variations in polymerase II activties in the oviduct of withdrawn chicks required that subsequent experiments include time-matched controls. The hormones alter RNA polymerase II and II activities in vivo as assayed in isolated nuclei. Progesterone represses the polymerase I and II activities, while estrogen alone and estrogen + progesterone enhance both polymerase activities immediately after injection. Diethylstillbestrol, a synthetic estrogen, causes changes similar to those of estrogen. The effects of these steroids on the polymerases are detected within 15 min of hormone injection. Changes in the capacities of chromatins to serve as template for RNA synthesis in general correlated with changes in polymerase II activities. Interestingly, in the case of estrogen treatment, the acidic chromatin protein (but not histone) levels fluctuate positively with the template capacities of the chromatin. An antagonism between estrogen and progesterone is observed in the responses of both RNA polymerases I and II activities as well as in the chromatin template capacity. Levels of messenger RNA coding for ovalbumin, as detected by hybridization with labeled complementary DNA, increase in oviducts of withdrawn chicks within 2--3 of the injection of estrogen, progesterone or estrogen + progesterone. This rapid accumulation of ovalbumin mRNA is not accompanied in each case by a similar increase in polymerase II activity or chromatin template capacity.", "contents": "Effects of estrogen and progesterone on transcription, chromatin and ovalbumin gene expression in the chick oviduct. The effects of estrogen, progesterone and estrogen + progesterone combined on nuclear transcriptional processes in oviducts of immature chicks, previously withdrawn from estrogen, are reported. The responses to the steroids of the endogenous nuclear RNA polymerase activities, both nucleolar (I) and nucleoplasmic (II), the chromatin compositions and template capacities, and the appearance of ovalbumin messenger RNA (mRNA) are compared. When immature chicks (previously treated at 14 days with estrogen) are withdrawn from estrogen treatment, there is a gradual reduction in both polymerase activities. Diurnal variations in polymerase II activties in the oviduct of withdrawn chicks required that subsequent experiments include time-matched controls. The hormones alter RNA polymerase II and II activities in vivo as assayed in isolated nuclei. Progesterone represses the polymerase I and II activities, while estrogen alone and estrogen + progesterone enhance both polymerase activities immediately after injection. Diethylstillbestrol, a synthetic estrogen, causes changes similar to those of estrogen. The effects of these steroids on the polymerases are detected within 15 min of hormone injection. Changes in the capacities of chromatins to serve as template for RNA synthesis in general correlated with changes in polymerase II activities. Interestingly, in the case of estrogen treatment, the acidic chromatin protein (but not histone) levels fluctuate positively with the template capacities of the chromatin. An antagonism between estrogen and progesterone is observed in the responses of both RNA polymerases I and II activities as well as in the chromatin template capacity. Levels of messenger RNA coding for ovalbumin, as detected by hybridization with labeled complementary DNA, increase in oviducts of withdrawn chicks within 2--3 of the injection of estrogen, progesterone or estrogen + progesterone. This rapid accumulation of ovalbumin mRNA is not accompanied in each case by a similar increase in polymerase II activity or chromatin template capacity.", "PMID": 952904}
{"id": "pubmed23n0032_297", "title": "Protein synthesis in dystrophic muscle. Activity of the pH 5 supernatant fraction of muscle in dystrophic mice.", "content": "The pH 5 supernatant fractions prepared from homogenates of tissues of normal and dystrophic mice were used to study the incorporation of [14C]phenylalanyl-tRNA into peptide. The incorpoation was markedly reduced using the muscle pH 5 supernatant fraction from dystrophic animals but no reduction was seen with brain, liver or heart preparations from dystrophic mice. The lower incorporation with dystrophic muscle pH 5 supernatant was not due to altered activity of ribonuclease, elongation factors, proteolytic enzymes, GTP or sulfhydryl reagents, but was attributable to the presence of activity that was inhibitory to protein synthesis.", "contents": "Protein synthesis in dystrophic muscle. Activity of the pH 5 supernatant fraction of muscle in dystrophic mice. The pH 5 supernatant fractions prepared from homogenates of tissues of normal and dystrophic mice were used to study the incorporation of [14C]phenylalanyl-tRNA into peptide. The incorpoation was markedly reduced using the muscle pH 5 supernatant fraction from dystrophic animals but no reduction was seen with brain, liver or heart preparations from dystrophic mice. The lower incorporation with dystrophic muscle pH 5 supernatant was not due to altered activity of ribonuclease, elongation factors, proteolytic enzymes, GTP or sulfhydryl reagents, but was attributable to the presence of activity that was inhibitory to protein synthesis.", "PMID": 952905}
{"id": "pubmed23n0032_298", "title": "Analysis of the human genome by complementary RNA/DNA gradient hybridization and relaxes cesium sulfate-silver ion density centrifugation.", "content": "3H-Labeled complementary RNA (cRNA) transcribed from total nuclear human DNA was hybridized to homologous DNA and the hybrids formed localized in CsC1 density gradients. Radioactive peaks indicative of cRNA/DNA hybrids identified ten density components. The experimental conditions used indicated that these components correspond to DNA rich in repeated sequences. In addition, cRNAs transcribed from five Cot fractions (31% of total DNA; Cot less than 100) were hybridized to total DNA in CsC1 gradients. The hybrids sedimented at similar densities to those seen in total cRNA but showed a differential distribution along the five fractions. To isolate native double-stranded DNA components containing the various families of repeated sequences observed, nuclear DNA was fractionated using relaxes Cs2SO4/Ag+ density gradient centrifugation. These fractions revealed the presence of seven major components a densities of 1.715, 1.711, 1.708, 1.705, 1.702, 1.700 and 1.698 g/cm3 and three minor components at densities of 1.696, 1.693 and 1.687 g/cm3. The density components corresponded to those observed by cRNA/DNA hybridization, thus suggesting that the bulk of the human genome is made up of ten density-biased components containing sequences of varying degrees of repetitiveness.", "contents": "Analysis of the human genome by complementary RNA/DNA gradient hybridization and relaxes cesium sulfate-silver ion density centrifugation. 3H-Labeled complementary RNA (cRNA) transcribed from total nuclear human DNA was hybridized to homologous DNA and the hybrids formed localized in CsC1 density gradients. Radioactive peaks indicative of cRNA/DNA hybrids identified ten density components. The experimental conditions used indicated that these components correspond to DNA rich in repeated sequences. In addition, cRNAs transcribed from five Cot fractions (31% of total DNA; Cot less than 100) were hybridized to total DNA in CsC1 gradients. The hybrids sedimented at similar densities to those seen in total cRNA but showed a differential distribution along the five fractions. To isolate native double-stranded DNA components containing the various families of repeated sequences observed, nuclear DNA was fractionated using relaxes Cs2SO4/Ag+ density gradient centrifugation. These fractions revealed the presence of seven major components a densities of 1.715, 1.711, 1.708, 1.705, 1.702, 1.700 and 1.698 g/cm3 and three minor components at densities of 1.696, 1.693 and 1.687 g/cm3. The density components corresponded to those observed by cRNA/DNA hybridization, thus suggesting that the bulk of the human genome is made up of ten density-biased components containing sequences of varying degrees of repetitiveness.", "PMID": 952906}
{"id": "pubmed23n0032_299", "title": "Temperature-induced perturbations in the circular dichroic spectrum of the synthetic polymer poly[d(G-C)].", "content": "The effect of increasing temperature on the double-helical synthetic DNA polymer poly[d(G-C)] with alternating base sequence, was studied by ultraviolet absorbance and circular dichroic techniques. While a cooperative hyperchromic effect was noticed in the ultraviolet absorbance spectrum above a temperature of 70 degrees C, an inversion of the circular dichroic spectrum was observed at a similar temperature range. On cooling to room temperature, the spectrum was reversed to the initial one. These observations, first reported here, are tentatively attributed to a conformational change of the polymer, from the right-handed double helix to the left-handed single strands, prior to complete denaturation.", "contents": "Temperature-induced perturbations in the circular dichroic spectrum of the synthetic polymer poly[d(G-C)]. The effect of increasing temperature on the double-helical synthetic DNA polymer poly[d(G-C)] with alternating base sequence, was studied by ultraviolet absorbance and circular dichroic techniques. While a cooperative hyperchromic effect was noticed in the ultraviolet absorbance spectrum above a temperature of 70 degrees C, an inversion of the circular dichroic spectrum was observed at a similar temperature range. On cooling to room temperature, the spectrum was reversed to the initial one. These observations, first reported here, are tentatively attributed to a conformational change of the polymer, from the right-handed double helix to the left-handed single strands, prior to complete denaturation.", "PMID": 952907}
{"id": "pubmed23n0032_300", "title": "Raman spectroscopic detection and examination of the interaction of amino acids, polypeptides and proteins with the phophatidylcholine lamellar structure.", "content": "Raman spectral peaks in the vicinity of 1100 and 2900 cm-1 for phosphatidylcholine were found to be sensitive to interactions with amino acids, polypeptides and plasma proteins. The amino acids L-luecine, L-isoleucine, L-tryptophan, L-arginine HCl, L-histidine HCl, L-threonine and L-aspartic acid decreased the dipalmitoyl phosphatidylcholine Raman intensity ratio  I1064/I1089 indicating an increase in the gauche hydrocarbon chain character of the lipid. The increase in the lipid approx. 2930 cm-1 peak intensity in relation to the approx. 2850 and approx. 2890 cm-1 peaks upon the addition of the amino acids L-arginine HCl, L-histidine-HCl and L-lysine-HCl to the lipid dispersion indicates that the lipid hydrocarbon chain environment becomes more polar in their presence. The lipid-alamethecin and lipid-valinomycin interactions produced a decrease in the lipid Raman intensity ratio I1064/I1089 again indicating an increase in the gauche hydrocarbon chain character of dicyristoyl phosphatidylcholine while producing no change in this ratio for dipalmitoyl phosphatidylcholine. Human fibrinogen and bovin serum albumin were found to increase the I2890/I2850 dimyristoyl phosphatidylcholine Raman intensity ratio while decreasing the I2850/I2930 dimyristoyl phosphatidylcholine Raman intensity ratio indicating that the lipid underwent a conformational change and that the hydrocarbon chain environment was more polar in the presence of albumin or fibrinogen.", "contents": "Raman spectroscopic detection and examination of the interaction of amino acids, polypeptides and proteins with the phophatidylcholine lamellar structure. Raman spectral peaks in the vicinity of 1100 and 2900 cm-1 for phosphatidylcholine were found to be sensitive to interactions with amino acids, polypeptides and plasma proteins. The amino acids L-luecine, L-isoleucine, L-tryptophan, L-arginine HCl, L-histidine HCl, L-threonine and L-aspartic acid decreased the dipalmitoyl phosphatidylcholine Raman intensity ratio  I1064/I1089 indicating an increase in the gauche hydrocarbon chain character of the lipid. The increase in the lipid approx. 2930 cm-1 peak intensity in relation to the approx. 2850 and approx. 2890 cm-1 peaks upon the addition of the amino acids L-arginine HCl, L-histidine-HCl and L-lysine-HCl to the lipid dispersion indicates that the lipid hydrocarbon chain environment becomes more polar in their presence. The lipid-alamethecin and lipid-valinomycin interactions produced a decrease in the lipid Raman intensity ratio I1064/I1089 again indicating an increase in the gauche hydrocarbon chain character of dicyristoyl phosphatidylcholine while producing no change in this ratio for dipalmitoyl phosphatidylcholine. Human fibrinogen and bovin serum albumin were found to increase the I2890/I2850 dimyristoyl phosphatidylcholine Raman intensity ratio while decreasing the I2850/I2930 dimyristoyl phosphatidylcholine Raman intensity ratio indicating that the lipid underwent a conformational change and that the hydrocarbon chain environment was more polar in the presence of albumin or fibrinogen.", "PMID": 952908}
{"id": "pubmed23n0032_301", "title": "31P NMR studies of unsonicated aqueous dispersions of neutral and acidic phospholipids. Effects of phase transitions, p2H and divalent cations on the motion in the phosphate region of the polar headgroup.", "content": "1. The 129 MHz (non-proton decoupled) and 36.4 MHz (proton decoupled) 31P NMR spectra arising from unsonicated aqueous dispersions of well defined species of phospholipid have been investigated. The phospholipids employed (and the parameters varied) include phosphatidylcholine (temperature), phosphatidylethanolamine (temperature), phosphatidic acid (temperature and p2H) and phosphatidylglycerol (temperature, p2H and Ca2+ (or Mg2+)) concentration. 2. At p2H = 7 the 31 P MNR spectra arising from saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol become progressively broader as the temperature is reduced below the phase transition, demonstrating reduced motion in the phosphate region of the polar headgroup. 3. In the liquid crystalline state at p2H = 7 the molecular dipolar order parameters obtained for saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol and very similar, and are independent of the acyl chain length for species derived from lauric and myristic acid. Thus the motion in the methylene-phosphate-methylene region is similar for these different liquid crystaline phospholipid species. 4. The 31 P NMR spectra of aqueous dispersions of 14:0/14:0 phosphatidic acid display anomalous temperature and p2H dependences. The effective chemical shift anistropy (delta v CSA EFF) at 5 degrees C varies from 71 ppm at p2H = 8.5 to 38 ppm at p2H = 2.5. Further, the motion in the phosphate region is relatively insensitive to the gel or liquid crystalline nature of the hydrocarbon chains. 5. The addition of 40 mol% Ca2+ (or Mg2+) to saturated species of phosphatidylglycerol causes an increase of approx. 20 degrees C in the hydrocarbon phase transition temperature as indicated by 31 P NMR. Equimolar concentrations of Ca2+ increase the transition temperature by approx. 70 degrees C, and no 31P NMR signal could be observed for the very condensed precipitate formed below this temperature. In the liquid crystalline state the motion in the phosphate region of the polar headgroup is not significantly affected by the presence of Ca+ or Mg2+. 6. The 31P NMR spectra obtained from 18 : 1c/18 : 1c phosphatidylethanolamine are consistent with a phase transition from a lamellar to an hexagonal (HII) phase in the region 10-15 degrees C. 7. The observed narrowing of the 31 P NMR spectra of aqueous dispersions of phospholipids as the temperature is raised toward the hydrocarbon transition temperature is discussed in terms of the \"pretransition\" observed in calorimetric studies.", "contents": "31P NMR studies of unsonicated aqueous dispersions of neutral and acidic phospholipids. Effects of phase transitions, p2H and divalent cations on the motion in the phosphate region of the polar headgroup. 1. The 129 MHz (non-proton decoupled) and 36.4 MHz (proton decoupled) 31P NMR spectra arising from unsonicated aqueous dispersions of well defined species of phospholipid have been investigated. The phospholipids employed (and the parameters varied) include phosphatidylcholine (temperature), phosphatidylethanolamine (temperature), phosphatidic acid (temperature and p2H) and phosphatidylglycerol (temperature, p2H and Ca2+ (or Mg2+)) concentration. 2. At p2H = 7 the 31 P MNR spectra arising from saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol become progressively broader as the temperature is reduced below the phase transition, demonstrating reduced motion in the phosphate region of the polar headgroup. 3. In the liquid crystalline state at p2H = 7 the molecular dipolar order parameters obtained for saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol and very similar, and are independent of the acyl chain length for species derived from lauric and myristic acid. Thus the motion in the methylene-phosphate-methylene region is similar for these different liquid crystaline phospholipid species. 4. The 31 P NMR spectra of aqueous dispersions of 14:0/14:0 phosphatidic acid display anomalous temperature and p2H dependences. The effective chemical shift anistropy (delta v CSA EFF) at 5 degrees C varies from 71 ppm at p2H = 8.5 to 38 ppm at p2H = 2.5. Further, the motion in the phosphate region is relatively insensitive to the gel or liquid crystalline nature of the hydrocarbon chains. 5. The addition of 40 mol% Ca2+ (or Mg2+) to saturated species of phosphatidylglycerol causes an increase of approx. 20 degrees C in the hydrocarbon phase transition temperature as indicated by 31 P NMR. Equimolar concentrations of Ca2+ increase the transition temperature by approx. 70 degrees C, and no 31P NMR signal could be observed for the very condensed precipitate formed below this temperature. In the liquid crystalline state the motion in the phosphate region of the polar headgroup is not significantly affected by the presence of Ca+ or Mg2+. 6. The 31P NMR spectra obtained from 18 : 1c/18 : 1c phosphatidylethanolamine are consistent with a phase transition from a lamellar to an hexagonal (HII) phase in the region 10-15 degrees C. 7. The observed narrowing of the 31 P NMR spectra of aqueous dispersions of phospholipids as the temperature is raised toward the hydrocarbon transition temperature is discussed in terms of the \"pretransition\" observed in calorimetric studies.", "PMID": 952909}
{"id": "pubmed23n0032_302", "title": "Kinetics and stability of alamethicin conducting channels in lipid bilayers.", "content": "It is already well-established that conduction in lipid bilayers containing alamethicin arises from the presence of complexes in which there are several molecules of the polypeptide. It is with the nature of these complexes that this paper is primarily concerned. While it is clear that increasing alamethicin concentration and increasing potential across the membrane favour their formation, the nature of the reactions involved has not yet been elucidated. Attempts have therefore been made to clarify the sequence of events leading to the establishment of a complex in its conducting state. It has been concluded that the most likely mechanism involves, initially, a non-field-dependent aggregation of the alamethicin, in the plane of the membrane, into non-conducting oligomers. These then appear to undergo movement normal to the membrane (which is field dependent) to form the conducting species. Temperature studies have shown that the various conducting states of the oligomer have effectively equal enthalpies, and that the activation energies for transitions between these states are all approx. 1.2kcal/mol. The corresponding rate constants are very sensitive to the lipid composition of the membrane and a variety of different systems has been examined in order to clarify the origins of this effect. The only conclusion from this part of the work is that lipid fluidity might be involved.", "contents": "Kinetics and stability of alamethicin conducting channels in lipid bilayers. It is already well-established that conduction in lipid bilayers containing alamethicin arises from the presence of complexes in which there are several molecules of the polypeptide. It is with the nature of these complexes that this paper is primarily concerned. While it is clear that increasing alamethicin concentration and increasing potential across the membrane favour their formation, the nature of the reactions involved has not yet been elucidated. Attempts have therefore been made to clarify the sequence of events leading to the establishment of a complex in its conducting state. It has been concluded that the most likely mechanism involves, initially, a non-field-dependent aggregation of the alamethicin, in the plane of the membrane, into non-conducting oligomers. These then appear to undergo movement normal to the membrane (which is field dependent) to form the conducting species. Temperature studies have shown that the various conducting states of the oligomer have effectively equal enthalpies, and that the activation energies for transitions between these states are all approx. 1.2kcal/mol. The corresponding rate constants are very sensitive to the lipid composition of the membrane and a variety of different systems has been examined in order to clarify the origins of this effect. The only conclusion from this part of the work is that lipid fluidity might be involved.", "PMID": 952910}
{"id": "pubmed23n0032_303", "title": "Studies on 6C3HED murine ascites tumor cell receptors for mannosyl-binding lectins.", "content": "We have investigated the receptor site activity present on 6C3HED tumor cells for concanavalin A, fava, lentil and pea lectins. The binding of the tritiated lectins to the tumor cells was inhibited by methyl-alpha-D-mannoside but not by D-galactose. The number of binding sites for the lectins was 3.5-10(6)/cell for concanavalin A, 3.3-10(6)/cell for fava, 3.6-10(6)/cell for lentil and 4.8-10(6)/cell for pea. The apparent association constants were 3.6 and 1.3 muM-1 for concanavalin A, 3.9 muM-1 for fava, 4.2 muM-1 for lentil and 4.6 and 0.6 muM-1 for pea. Competitive inhibition studies showed that lentil was a good inhibitor of pea binding; concanavalin A was a poor inhibitor of pea binding; and fava was a better inhibitor than concanavalin A but not as good as lentil. Reciprocal inhibition experiments indicated that concanavalin A and pea may bind to different receptors as well as to common receptors. This was also indicated by the observation that trypsin or protease treatment of the cells decreased the binding of pea lectin by 20-40 percent whereas concanavalin A binding was unaffected.", "contents": "Studies on 6C3HED murine ascites tumor cell receptors for mannosyl-binding lectins. We have investigated the receptor site activity present on 6C3HED tumor cells for concanavalin A, fava, lentil and pea lectins. The binding of the tritiated lectins to the tumor cells was inhibited by methyl-alpha-D-mannoside but not by D-galactose. The number of binding sites for the lectins was 3.5-10(6)/cell for concanavalin A, 3.3-10(6)/cell for fava, 3.6-10(6)/cell for lentil and 4.8-10(6)/cell for pea. The apparent association constants were 3.6 and 1.3 muM-1 for concanavalin A, 3.9 muM-1 for fava, 4.2 muM-1 for lentil and 4.6 and 0.6 muM-1 for pea. Competitive inhibition studies showed that lentil was a good inhibitor of pea binding; concanavalin A was a poor inhibitor of pea binding; and fava was a better inhibitor than concanavalin A but not as good as lentil. Reciprocal inhibition experiments indicated that concanavalin A and pea may bind to different receptors as well as to common receptors. This was also indicated by the observation that trypsin or protease treatment of the cells decreased the binding of pea lectin by 20-40 percent whereas concanavalin A binding was unaffected.", "PMID": 952911}
{"id": "pubmed23n0032_304", "title": "A simple model for calcium induced exocytosis.", "content": "We have developed a simple model showing how the presence or obsence of Ca2+ can determine whether an uncurved or curved membrane surface is favored energetically. The model shows why fusion of vesicles with the presynaptic membrane is favored in the presence of calcium and why the budding off of vesicles is favored in the absence of calcium inside of the presynaptic membrane. The model accurately predicts the radius of a synaptic vesicle using known properties of lipids and suggests consequences of temperature change, varied stimulation rate and addition of calcium by artificial means on rates of transmitter release.", "contents": "A simple model for calcium induced exocytosis. We have developed a simple model showing how the presence or obsence of Ca2+ can determine whether an uncurved or curved membrane surface is favored energetically. The model shows why fusion of vesicles with the presynaptic membrane is favored in the presence of calcium and why the budding off of vesicles is favored in the absence of calcium inside of the presynaptic membrane. The model accurately predicts the radius of a synaptic vesicle using known properties of lipids and suggests consequences of temperature change, varied stimulation rate and addition of calcium by artificial means on rates of transmitter release.", "PMID": 952912}
{"id": "pubmed23n0032_305", "title": "Role of calcium in exocrine pancreatic secretion. III. Comparison of calcium and magnesium movements in rabbit pancreas.", "content": "1. Calcium and magnesium movements in the isolated rabbit pancreas and in rabbit pancreas fragments are compared in a qualitative and quantitative way. 2. At the basal secretion rate calcium and magnesium are present in the secreted fluid in concentrations of about 30% of their concentrations in the bathing medium. 3. Addition of 10(-6) M carbachol to the bathing medium results in enzyme secretion accompanied by calcium and magnesium release, in divalent cation-free medium as well as in a complete medium. 4. The secretion of each divalent cation is the sum of two components: an extracellular flux and a flux of protein-associated cations, the so-called secretory flux. 5. The extracellular flux is proportional to the concentration of the divalent cation in the bathing medium. The secretory flux is not dependent on the presence of the divalent cation in the bathing medium, but is proportional to the amount of protein secreted. About 25 nmol of each cation is secreted per mg protein. 6. Ca2+ and Mg2+ can be nearly completely separated from the digestive enzymes by gel filtration. They equilibrate completely with their radioactive isotopes added to the sample just before elution, indicating that the cations are rapidly exchangeable after secretion. 7. Efflux studies on rabbit pancreas fragments, pre-loaded with 45Ca2+, show a carbachol-stimulated 45Ca2+ efflux (the stimulatory flux) in addition to a release of amylase. Fragments pre-loaded with 28Mg2+ do not show carbachol stimulation of the tracer efflux. 8. These studies indicate that calcium and magnesium behave quite similarly with respect to the extracellular and secretory fluxes. The absence of a stimulatory flux for magnesium, suggests that the increase of the cytoplasmic calcium concentration plays a specific role in the stimulus-secretion coupling of pancreatic enzyme secretion.", "contents": "Role of calcium in exocrine pancreatic secretion. III. Comparison of calcium and magnesium movements in rabbit pancreas. 1. Calcium and magnesium movements in the isolated rabbit pancreas and in rabbit pancreas fragments are compared in a qualitative and quantitative way. 2. At the basal secretion rate calcium and magnesium are present in the secreted fluid in concentrations of about 30% of their concentrations in the bathing medium. 3. Addition of 10(-6) M carbachol to the bathing medium results in enzyme secretion accompanied by calcium and magnesium release, in divalent cation-free medium as well as in a complete medium. 4. The secretion of each divalent cation is the sum of two components: an extracellular flux and a flux of protein-associated cations, the so-called secretory flux. 5. The extracellular flux is proportional to the concentration of the divalent cation in the bathing medium. The secretory flux is not dependent on the presence of the divalent cation in the bathing medium, but is proportional to the amount of protein secreted. About 25 nmol of each cation is secreted per mg protein. 6. Ca2+ and Mg2+ can be nearly completely separated from the digestive enzymes by gel filtration. They equilibrate completely with their radioactive isotopes added to the sample just before elution, indicating that the cations are rapidly exchangeable after secretion. 7. Efflux studies on rabbit pancreas fragments, pre-loaded with 45Ca2+, show a carbachol-stimulated 45Ca2+ efflux (the stimulatory flux) in addition to a release of amylase. Fragments pre-loaded with 28Mg2+ do not show carbachol stimulation of the tracer efflux. 8. These studies indicate that calcium and magnesium behave quite similarly with respect to the extracellular and secretory fluxes. The absence of a stimulatory flux for magnesium, suggests that the increase of the cytoplasmic calcium concentration plays a specific role in the stimulus-secretion coupling of pancreatic enzyme secretion.", "PMID": 952913}
{"id": "pubmed23n0032_306", "title": "Hydrophobic affinity partition of spinach chloroplasts in aqueous two-phase systems.", "content": "The surface properties of spinach chloroplasts, both of intact chloroplasts with surrounding envelope and broken chloroplasts consisting of the inner lamellar system, have been studied by partitioning them between two aqueous phases, especially using counter-current distribution technique. The two-phase system consists of poly(ethyleneglycol), dextran and water. The two polymers are enriched in opposite phases and by binding deoxycholate or palmitate to one of the polymers the affinity of chloroplasts for the corresponding phase is strongly enhanced. The partition of the two classes of chloroplasts, however, is not affected to the same degree and the affinity of the chloroplast envelope for deoxycholate and palmitate is stronger than that of the lamellar system. This has been correlated to the chemical composition of the two types of membranes. By studying the effect of salts on the partition it has been found that the lamellar system bears a larger number of negative charges as compared to the envelope of the intact chloroplast.", "contents": "Hydrophobic affinity partition of spinach chloroplasts in aqueous two-phase systems. The surface properties of spinach chloroplasts, both of intact chloroplasts with surrounding envelope and broken chloroplasts consisting of the inner lamellar system, have been studied by partitioning them between two aqueous phases, especially using counter-current distribution technique. The two-phase system consists of poly(ethyleneglycol), dextran and water. The two polymers are enriched in opposite phases and by binding deoxycholate or palmitate to one of the polymers the affinity of chloroplasts for the corresponding phase is strongly enhanced. The partition of the two classes of chloroplasts, however, is not affected to the same degree and the affinity of the chloroplast envelope for deoxycholate and palmitate is stronger than that of the lamellar system. This has been correlated to the chemical composition of the two types of membranes. By studying the effect of salts on the partition it has been found that the lamellar system bears a larger number of negative charges as compared to the envelope of the intact chloroplast.", "PMID": 952914}
{"id": "pubmed23n0032_307", "title": "An x-ray study of the condensed and separated states of sciatic nerve myelin.", "content": "Low-angle X-ray diffraction patterns of peripheral nerve myelin after modification by either rehydration in various solutions or by chemical treatment have been recorded. These X-ray patterns and the previously reported modified nerve myelin patterns demonstrate that nerve myelin has at least five different states: the normal state, condensed state I and II and separated state I and II. There are two membranes per unit cell in the normal state and in states II whereas there is one membrane per unit cell in states I. Under certain conditions normal nerve can go reversibly into either of states II. With continued treatment the nerve myelin structure moves irreversibly from state II to state I and, once in state I, the nerve myelin layers cannot return to the normal state. Our results demonstrate that there is a reversible transformation between condensed state I and separated state I. Fourier profiles of nerve myelin in the normal state, condensed state I and separated state I are presented.", "contents": "An x-ray study of the condensed and separated states of sciatic nerve myelin. Low-angle X-ray diffraction patterns of peripheral nerve myelin after modification by either rehydration in various solutions or by chemical treatment have been recorded. These X-ray patterns and the previously reported modified nerve myelin patterns demonstrate that nerve myelin has at least five different states: the normal state, condensed state I and II and separated state I and II. There are two membranes per unit cell in the normal state and in states II whereas there is one membrane per unit cell in states I. Under certain conditions normal nerve can go reversibly into either of states II. With continued treatment the nerve myelin structure moves irreversibly from state II to state I and, once in state I, the nerve myelin layers cannot return to the normal state. Our results demonstrate that there is a reversible transformation between condensed state I and separated state I. Fourier profiles of nerve myelin in the normal state, condensed state I and separated state I are presented.", "PMID": 952915}
{"id": "pubmed23n0032_308", "title": "Outside-inside distributions and sizes of mixed phosphatidylcholine-cholesterol vesicles.", "content": "(1) The effect of the incorporation of cholesterol upon the distribution of various molecular species of phosphatidylcholine across the bilayers of mixed sonicated liposomes (vesicles) has been studied with 31P-MNR. (2) The outside-inside ratio of both saturated and unsaturated phosphatidylcholine species was not much affected by the incorporation of up to 30 mol% cholesterol. Above 30 mol% cholesterol the outside-inside ratio strongly increased for phosphatidylcholines with cis unsaturated fatty acid chains. In contrast the outside-inside ratio of trans unsaturated and fully saturated phophatidylcholine species was either not affected or decreased by the incorporation of more than 30 mol% cholesterol. (3) a simple relationship between the size of the vesicle and the linewidth of the 31P-NMR resonance is described. From the measured linewidths the sizes of the various cholesterol containing vesicles have been obtained. It is found that incorporation of 0-30 mol% cholesterol does not significantly affect the size of the vesicle whereas above 30 mol% cholesterol does not significantly affect the size of the vesicle whereas above 30 mol% cholesterol the size of all phosphatidylcholine vesicles sharply increases. The increase in size is the largest for the more saturated phosphatidylcholine species. (4) From the outside-inside ratio and the size of the vesicle the composition of the outer and inner layer of the mixed vesicles could be obtained. Below 30 mol% cholesterol the composition of outer and inner layer is nearly identical. Above 30 mol% cholesterol the distribution of lipid across the bilayer of all visicles becomes assymetric with a disporportionately larger amount of cholesterol present in the inside monolayer.", "contents": "Outside-inside distributions and sizes of mixed phosphatidylcholine-cholesterol vesicles. (1) The effect of the incorporation of cholesterol upon the distribution of various molecular species of phosphatidylcholine across the bilayers of mixed sonicated liposomes (vesicles) has been studied with 31P-MNR. (2) The outside-inside ratio of both saturated and unsaturated phosphatidylcholine species was not much affected by the incorporation of up to 30 mol% cholesterol. Above 30 mol% cholesterol the outside-inside ratio strongly increased for phosphatidylcholines with cis unsaturated fatty acid chains. In contrast the outside-inside ratio of trans unsaturated and fully saturated phophatidylcholine species was either not affected or decreased by the incorporation of more than 30 mol% cholesterol. (3) a simple relationship between the size of the vesicle and the linewidth of the 31P-NMR resonance is described. From the measured linewidths the sizes of the various cholesterol containing vesicles have been obtained. It is found that incorporation of 0-30 mol% cholesterol does not significantly affect the size of the vesicle whereas above 30 mol% cholesterol does not significantly affect the size of the vesicle whereas above 30 mol% cholesterol the size of all phosphatidylcholine vesicles sharply increases. The increase in size is the largest for the more saturated phosphatidylcholine species. (4) From the outside-inside ratio and the size of the vesicle the composition of the outer and inner layer of the mixed vesicles could be obtained. Below 30 mol% cholesterol the composition of outer and inner layer is nearly identical. Above 30 mol% cholesterol the distribution of lipid across the bilayer of all visicles becomes assymetric with a disporportionately larger amount of cholesterol present in the inside monolayer.", "PMID": 952917}
{"id": "pubmed23n0032_309", "title": "Saccharides on teratocarcinoma cell plasma membranes. Their investigation with radioactively labelled lectins.", "content": "We have studied the interaction of five lectins differing in their sugar specificity, with the surface of clonal cell lines derived from transplantable murine teratocarcinoma. The results show that the differentiation from primitive embryonal carcinoma cells into parietal yolk sac cells is accompanied by changes in cell surface saccharides. These changes consist of a marked decrease in the total number of binding sites for the L-fucose-specific lectin of Lotus tetragonolobus and a large increase in the total number of binding sites for wax bean agglutinin. It is suggested that these differences can be used as markers in the study of this early embryonic differentiation. No agglutination of primitive embryonal carcinoma cells or of parietal yolk sac cells by low concentrations (10mug/ml) of concanavalin A, soybean agglutinin or the fucose binding proteins was observed.", "contents": "Saccharides on teratocarcinoma cell plasma membranes. Their investigation with radioactively labelled lectins. We have studied the interaction of five lectins differing in their sugar specificity, with the surface of clonal cell lines derived from transplantable murine teratocarcinoma. The results show that the differentiation from primitive embryonal carcinoma cells into parietal yolk sac cells is accompanied by changes in cell surface saccharides. These changes consist of a marked decrease in the total number of binding sites for the L-fucose-specific lectin of Lotus tetragonolobus and a large increase in the total number of binding sites for wax bean agglutinin. It is suggested that these differences can be used as markers in the study of this early embryonic differentiation. No agglutination of primitive embryonal carcinoma cells or of parietal yolk sac cells by low concentrations (10mug/ml) of concanavalin A, soybean agglutinin or the fucose binding proteins was observed.", "PMID": 952918}
{"id": "pubmed23n0032_310", "title": "Alteration of birefringence signals from squid giant axons by intracellular perfusion with protease solution.", "content": "The optical signal, arising from a transient birefringence change associated with excitation, was recorded from a squid giant axon together with the membrane potential change, and the effect of removal of the axoplasm on the optical signal was examined. In an unperfused axon, repetitive stimulation at a frequency of about 100 Hz produced two kinds of optical response. The initial response had a brief, spike-like time course and was elicited by each stimulating pulse. The delayed response had a slow time course and the sign of decreased light intensity, and summated with repetitive stimulation. Most of the axoplasm was removed from interior of the axon by intracellular perfusion with solutions containing pronase at a concentration of 0.1 mg/ml. The delayed response could selectively be eliminated by perfusion with a pronase-containing solution for 2-8 min. The result was interpreted as showing that the delayed birefringence signal originates from axoplasm when its gel structure was transiently disturbed by an increased Ca2+ influx associated with excitation. When perfusion was further continued the duration of the action potential started increasing and often a prominent after-depolarization appeared. At this stage the initial optical response was again followed by a large slow signal with the sign of increased light intensity. This reversed delayed response was tentatively assumed to originate from the membrane with some remaining axoplasm, but its cause is still not understood.", "contents": "Alteration of birefringence signals from squid giant axons by intracellular perfusion with protease solution. The optical signal, arising from a transient birefringence change associated with excitation, was recorded from a squid giant axon together with the membrane potential change, and the effect of removal of the axoplasm on the optical signal was examined. In an unperfused axon, repetitive stimulation at a frequency of about 100 Hz produced two kinds of optical response. The initial response had a brief, spike-like time course and was elicited by each stimulating pulse. The delayed response had a slow time course and the sign of decreased light intensity, and summated with repetitive stimulation. Most of the axoplasm was removed from interior of the axon by intracellular perfusion with solutions containing pronase at a concentration of 0.1 mg/ml. The delayed response could selectively be eliminated by perfusion with a pronase-containing solution for 2-8 min. The result was interpreted as showing that the delayed birefringence signal originates from axoplasm when its gel structure was transiently disturbed by an increased Ca2+ influx associated with excitation. When perfusion was further continued the duration of the action potential started increasing and often a prominent after-depolarization appeared. At this stage the initial optical response was again followed by a large slow signal with the sign of increased light intensity. This reversed delayed response was tentatively assumed to originate from the membrane with some remaining axoplasm, but its cause is still not understood.", "PMID": 952919}
{"id": "pubmed23n0032_311", "title": "Physicochemical studies of taste reception. III. Interpretation of the water response in taste reception.", "content": "The model membrane composed of a Millipore filter paper and the total lipids from bovine tongue epithelium or phosphatidylcholine from egg yolk simulated well the water response of a living taste cell, The water response observed with the model membrane adapted to various salt solutions was interpreted in terms of changes in electric potential at the membrane-solution interface, i.e. the water response was attributed to the e.m.f. change produced by diffusion of the electrolytes dissolved in (or adsorbed on) the membrane surface into the bulk solution. The water response of the frog tongue was also investigated by measuring the neural response of the glossopharyngeal nerve. The results obtained were consistent with the mechanism proposed in the present paper. The response of the frog to Ca2+ was examined under the condition where the water response was suppressed, and it was concluded that the water response of the frog is different from the response to Ca2+.", "contents": "Physicochemical studies of taste reception. III. Interpretation of the water response in taste reception. The model membrane composed of a Millipore filter paper and the total lipids from bovine tongue epithelium or phosphatidylcholine from egg yolk simulated well the water response of a living taste cell, The water response observed with the model membrane adapted to various salt solutions was interpreted in terms of changes in electric potential at the membrane-solution interface, i.e. the water response was attributed to the e.m.f. change produced by diffusion of the electrolytes dissolved in (or adsorbed on) the membrane surface into the bulk solution. The water response of the frog tongue was also investigated by measuring the neural response of the glossopharyngeal nerve. The results obtained were consistent with the mechanism proposed in the present paper. The response of the frog to Ca2+ was examined under the condition where the water response was suppressed, and it was concluded that the water response of the frog is different from the response to Ca2+.", "PMID": 952920}
{"id": "pubmed23n0032_312", "title": "An optical study of the exchange  kinetics of membrane bound molecules.", "content": "The kinetics of molecular exchange between lipid bilayers are studied using a special fluorescence technique. Pyrene and pyrene decanoic acid are chosen as typical examples of an apolar and amphiphilic molecule. Their property of forming dimers in the excited state (excimer) is exploited. The time dependencies of monomer and excimer intensities after rapid mixing of vesicles doped with fluorescent probe with undoped ones are studied by stopped-flow technique. The transient curves reveal the information on the exchange kinetics. A theoretical analysis shows that the molecular exchange follows a first order kinetics. Surprisingly short half life-times tex for this exchange process are obtained (for dipalmitoyl phosphatidylcholine tex=3.3 s for T=23 degrees C, tex=0.5 s for T=68 degrees C). Multilamellar systems (onion like structure) show much slower exchange rates. The exchange rates are nearly equal for polar and unpolar molecules. Addition of cholesterol has a strong reducing effect on this rate. Charging of dipalmitoyl phosphatidylcholine vesicle surfaces by the addition of (a) EuCl3 to the aqueous phase and (b) dipalmitoyl phosphatidic acid to the lipid phase reduces the exchange rate by about an order of magnitude above the phase transition. In a separate experiment it is shown that the lipid exchange or fusion for two different lipids is a much slower process compared to the label exchange. In fact vesicles kept below the phase transition temperature Ttr for both lipids, do not fuse even after 70 h. Noticeable fusion occurs after 10 h when the mixture stays above Ttr. Experiment shows that the fusion of pure lipid vesicles is not very much affected by the presence of a charged lipid. Change in concentration of the monovalent ions in the aqueous solution by two orders of magnitude does not have an appreciable effect on the exchange rate of phospholipids.", "contents": "An optical study of the exchange  kinetics of membrane bound molecules. The kinetics of molecular exchange between lipid bilayers are studied using a special fluorescence technique. Pyrene and pyrene decanoic acid are chosen as typical examples of an apolar and amphiphilic molecule. Their property of forming dimers in the excited state (excimer) is exploited. The time dependencies of monomer and excimer intensities after rapid mixing of vesicles doped with fluorescent probe with undoped ones are studied by stopped-flow technique. The transient curves reveal the information on the exchange kinetics. A theoretical analysis shows that the molecular exchange follows a first order kinetics. Surprisingly short half life-times tex for this exchange process are obtained (for dipalmitoyl phosphatidylcholine tex=3.3 s for T=23 degrees C, tex=0.5 s for T=68 degrees C). Multilamellar systems (onion like structure) show much slower exchange rates. The exchange rates are nearly equal for polar and unpolar molecules. Addition of cholesterol has a strong reducing effect on this rate. Charging of dipalmitoyl phosphatidylcholine vesicle surfaces by the addition of (a) EuCl3 to the aqueous phase and (b) dipalmitoyl phosphatidic acid to the lipid phase reduces the exchange rate by about an order of magnitude above the phase transition. In a separate experiment it is shown that the lipid exchange or fusion for two different lipids is a much slower process compared to the label exchange. In fact vesicles kept below the phase transition temperature Ttr for both lipids, do not fuse even after 70 h. Noticeable fusion occurs after 10 h when the mixture stays above Ttr. Experiment shows that the fusion of pure lipid vesicles is not very much affected by the presence of a charged lipid. Change in concentration of the monovalent ions in the aqueous solution by two orders of magnitude does not have an appreciable effect on the exchange rate of phospholipids.", "PMID": 952921}
{"id": "pubmed23n0032_313", "title": "The effect of hashish compounds on pphospholipid phase transition.", "content": "The interaction of hashish compounds, delta 1-tetrahydrocannabinol and cannabidiol, with dipalmitoyl phosphatidylcholine was investigated using differential scanning calorimetry. Both drugs affect the transition of dipalmitoyl phosphatidylcholine from the gel to liquid crystalline state, decreasing both the melting temperature and the enthalpy of melting. At a drug to dipalmitoyl phosphatidylcholine ratio of approx. 1:5, two peaks appear in the transition profile, suggesting a phase separation in the drug dipalmitoyl phosphatidylcholine mixture.", "contents": "The effect of hashish compounds on pphospholipid phase transition. The interaction of hashish compounds, delta 1-tetrahydrocannabinol and cannabidiol, with dipalmitoyl phosphatidylcholine was investigated using differential scanning calorimetry. Both drugs affect the transition of dipalmitoyl phosphatidylcholine from the gel to liquid crystalline state, decreasing both the melting temperature and the enthalpy of melting. At a drug to dipalmitoyl phosphatidylcholine ratio of approx. 1:5, two peaks appear in the transition profile, suggesting a phase separation in the drug dipalmitoyl phosphatidylcholine mixture.", "PMID": 952922}
{"id": "pubmed23n0032_314", "title": "Semlike Forest virus membrane proteins. Preparation and characterization of spike complexes soluble in detergent-free medium.", "content": "After Triton X-100 delipidation and subsequent Triton X-100 removal in a sucrose gradient the membrane protein spikes of Semliki Forest virus remained soluble in aqueous buffers. It was shown they were present as octameric complexes with a molecular weight of 95-10(4) and that they contain less than 4% lipid and detergent by weight. In electron microscopy after negative staining they appeared as \"rosette\"-shaped particles. Part of the protein could also be found associated in ordered paracrystalline arrays.", "contents": "Semlike Forest virus membrane proteins. Preparation and characterization of spike complexes soluble in detergent-free medium. After Triton X-100 delipidation and subsequent Triton X-100 removal in a sucrose gradient the membrane protein spikes of Semliki Forest virus remained soluble in aqueous buffers. It was shown they were present as octameric complexes with a molecular weight of 95-10(4) and that they contain less than 4% lipid and detergent by weight. In electron microscopy after negative staining they appeared as \"rosette\"-shaped particles. Part of the protein could also be found associated in ordered paracrystalline arrays.", "PMID": 952923}
{"id": "pubmed23n0032_315", "title": "Isolation and characterization of three unusual membrane glycopeptides present in rat intestinal endoplasmic reticula.", "content": "Three glycopeptides have been isolated from the mucosal homogenates of the rat small intestine without using proteolysis. These glycopeptides appear to be localized exclusively in the membranes of the endoplasmic reticula. Although they have similar molecular weights of about 2550 and have similar amino acid compositions, they differ in the carbohydrate constituents. The major glycopeptide has 2 mol glucose per polypeptide chain while the two other glycopeptides contain 1 mol fucose, mannose and galactose with either 1 or 2 mol glucose. No hexosamine or sialic acid was detected in any of the glycopeptides. An unusual physical property was found associated with these glycopeptides. Below pH 6.5 they formed a precipitate which prevented them from  diffusing through a dialysis membrane and allowed them to be rapidly purified following solubilization from the membrane. These glycopeptides appear to represent a new group of heretofore uncharacterized membrane constitutents which may play a role in some function specific for the endoplasmic reticula.", "contents": "Isolation and characterization of three unusual membrane glycopeptides present in rat intestinal endoplasmic reticula. Three glycopeptides have been isolated from the mucosal homogenates of the rat small intestine without using proteolysis. These glycopeptides appear to be localized exclusively in the membranes of the endoplasmic reticula. Although they have similar molecular weights of about 2550 and have similar amino acid compositions, they differ in the carbohydrate constituents. The major glycopeptide has 2 mol glucose per polypeptide chain while the two other glycopeptides contain 1 mol fucose, mannose and galactose with either 1 or 2 mol glucose. No hexosamine or sialic acid was detected in any of the glycopeptides. An unusual physical property was found associated with these glycopeptides. Below pH 6.5 they formed a precipitate which prevented them from  diffusing through a dialysis membrane and allowed them to be rapidly purified following solubilization from the membrane. These glycopeptides appear to represent a new group of heretofore uncharacterized membrane constitutents which may play a role in some function specific for the endoplasmic reticula.", "PMID": 952924}
{"id": "pubmed23n0032_316", "title": "Some characteristics of new tissue-binding proteins for metabolites of vitamin D other than 1,25-dihydroxyvitamin D.", "content": "Protein(s) have been found in a wide range of tissues which have a high affinity for 25-hydroxycholecalciferol. Of the tissues examined only erythrocytes do not have this protein. The properties of the protein have been examined and it has been found that the association constatns range from 2 - 10(9) to 5 - 10(9) M-1 and the sedimentation constants between 5.0 and 6.0 S. It was not possible to distinguish the proteins from the different tissues by their S values, mobility on gel electrophoresis or behaviour on ion-exchange chromatography. These techniques were all used, however, to show that the tissue 25-hydroxycholecalciferol binding protein is distinct from the main plasma binding protein for this steroid and from the intestinal 1,25-dihydroxycholecalciferol-binding protein. A protein has been in the plasma of rachitic animals but not of normals, which is apparently indistinguishable from this new tissue 25-hydroxycholecalciferol-binding protein. The steroid specificity of this new binding protein has been shown to be dependent upon a C-25 hydroxyl group, and an intact conjugated double bond system. Possible functions for this protein have been briefly discussed.", "contents": "Some characteristics of new tissue-binding proteins for metabolites of vitamin D other than 1,25-dihydroxyvitamin D. Protein(s) have been found in a wide range of tissues which have a high affinity for 25-hydroxycholecalciferol. Of the tissues examined only erythrocytes do not have this protein. The properties of the protein have been examined and it has been found that the association constatns range from 2 - 10(9) to 5 - 10(9) M-1 and the sedimentation constants between 5.0 and 6.0 S. It was not possible to distinguish the proteins from the different tissues by their S values, mobility on gel electrophoresis or behaviour on ion-exchange chromatography. These techniques were all used, however, to show that the tissue 25-hydroxycholecalciferol binding protein is distinct from the main plasma binding protein for this steroid and from the intestinal 1,25-dihydroxycholecalciferol-binding protein. A protein has been in the plasma of rachitic animals but not of normals, which is apparently indistinguishable from this new tissue 25-hydroxycholecalciferol-binding protein. The steroid specificity of this new binding protein has been shown to be dependent upon a C-25 hydroxyl group, and an intact conjugated double bond system. Possible functions for this protein have been briefly discussed.", "PMID": 952925}
{"id": "pubmed23n0032_317", "title": "N-Acetylgalactosamine 4,6-bissulfate in rat urine. I. Isolation, identification and chemical synthesis.", "content": "By starting with 4 1 of rat urine, it was possible to obtain a sulfate ester of hexosamine in crystalline form. A series of identification procedures including chemical analyses, enzymatic digestion, proton magnetic resonance spectroscopy and infrared spectroscopy showed that this substance is 2-acetamido-2-deoxy-D-galactose 4,6-bissulfate. The trivial name for this compound is N-acetylgalactosamine 4,6-bissulfate; Quantitation by isotopic techniques indicated the urine possessed an average concentration of 8 muM N-acetylgalactosamine 4,6-bissulfate. Further extension of these studies necessitated the chemical synthesis of N-acetylgalactosamine 4,6-bissulfate and related compounds to be used for references or as biological substrates. Direct sulfation of N-acetylgalactosamine was attempted first, and strong preference for attack on the primary hydroxyl group (position 6) was found for chlorosulfonic acid. Thus, the reaction with 2.2 molar equivalents of the sulfating agent gave N-acetylgalactosamine 6-sulfate and its derivatives bearing a second sulfate at either position 1 (minor) or position 3 (major). The lack of sulfation at position 4 could be attributed to steric effects of the sulfate group preferentially attached to position 6. Another experiment in which UDP-N-acetylgalactosamine 4-sulfate was used in place of the free sugar led to the formation of a bissulfated sugar-nucleotide which, on subsequent hydrolysis with mild acid, afforded N-acetylgalactosamine 4,6-bissulfate, the same compound as that obtained from rat urine.", "contents": "N-Acetylgalactosamine 4,6-bissulfate in rat urine. I. Isolation, identification and chemical synthesis. By starting with 4 1 of rat urine, it was possible to obtain a sulfate ester of hexosamine in crystalline form. A series of identification procedures including chemical analyses, enzymatic digestion, proton magnetic resonance spectroscopy and infrared spectroscopy showed that this substance is 2-acetamido-2-deoxy-D-galactose 4,6-bissulfate. The trivial name for this compound is N-acetylgalactosamine 4,6-bissulfate; Quantitation by isotopic techniques indicated the urine possessed an average concentration of 8 muM N-acetylgalactosamine 4,6-bissulfate. Further extension of these studies necessitated the chemical synthesis of N-acetylgalactosamine 4,6-bissulfate and related compounds to be used for references or as biological substrates. Direct sulfation of N-acetylgalactosamine was attempted first, and strong preference for attack on the primary hydroxyl group (position 6) was found for chlorosulfonic acid. Thus, the reaction with 2.2 molar equivalents of the sulfating agent gave N-acetylgalactosamine 6-sulfate and its derivatives bearing a second sulfate at either position 1 (minor) or position 3 (major). The lack of sulfation at position 4 could be attributed to steric effects of the sulfate group preferentially attached to position 6. Another experiment in which UDP-N-acetylgalactosamine 4-sulfate was used in place of the free sugar led to the formation of a bissulfated sugar-nucleotide which, on subsequent hydrolysis with mild acid, afforded N-acetylgalactosamine 4,6-bissulfate, the same compound as that obtained from rat urine.", "PMID": 952926}
{"id": "pubmed23n0032_318", "title": "N-Acetylgalactosamine 4,6-bissulfate in rat urine. II. Occurrence in rat cartilage and blood.", "content": "The intraperitoneal injection of inorganic [35S]sulfate to rat was followed by the rapid appearance in urine of a labeled compound which behaved as N-acetylgalactosamine 4,6-bissulfate on paper chromatography and paper electrophoresis and when treated with two sulfatases with a high degree of specificity toward the sulfate bonds at positions 4 and 6, respectively. Enzymatically-prepared N-acetylgalactosamine 4,6-[6-35S]bissulfate was injected intravenously into rats. Of the injected dose, 90% was excreted unchanged in the urine during the subsequent 12 h, suggesting that the urinary N-acetylgalactosamine 4,6-bissulfate may derive from blood as renal filtrate. Examination of the rats injected with inorganic [35S]sulfate revealed the presence of labeled N-acetylgalactosamine 4,6-bissulfate at significant levels in the blood and cartilage, but at much lower levels in the liver. The cartilage component was highest in its rate of 35S uptake, suggesting that the blood component may derive at least in some part from the cartilage. Exposure of surviving cartilage slices to inorganic [35S]sulfate, followed by extraction of the slices with hot 50% ethanol yielded a number of radioactive compounds, of which three were characterized as UDP-N-acetylgalactosamine-4,6-[35S]bissulfate, N-acetylgalactosamine-1-phosphate 4,6-[35S]bissulfate and N-acetylgalactosamine 4,6-[35S]bissulfate. By subjecting the prelabeled tissue to chase incubation, it was possible to show that the UDP-N-acetylgalactosamine-4,6-bissulfate in the tissue disappeared with an approximate half-life of 10 min with a concomitant appearance in the medium of N-acetylgalactosamine 4,6-bissulfate and its 1-phosphate ester. These results suggest the occurrence in cartilage of an enzymatic system which is responsible for rapid turnover of UDP-N-acetylgalactosamine-4,6-bissulfate and possibly required for the rapid secretion of N-acetylgalactosamine 4,6-bissulfate into extracellular field.", "contents": "N-Acetylgalactosamine 4,6-bissulfate in rat urine. II. Occurrence in rat cartilage and blood. The intraperitoneal injection of inorganic [35S]sulfate to rat was followed by the rapid appearance in urine of a labeled compound which behaved as N-acetylgalactosamine 4,6-bissulfate on paper chromatography and paper electrophoresis and when treated with two sulfatases with a high degree of specificity toward the sulfate bonds at positions 4 and 6, respectively. Enzymatically-prepared N-acetylgalactosamine 4,6-[6-35S]bissulfate was injected intravenously into rats. Of the injected dose, 90% was excreted unchanged in the urine during the subsequent 12 h, suggesting that the urinary N-acetylgalactosamine 4,6-bissulfate may derive from blood as renal filtrate. Examination of the rats injected with inorganic [35S]sulfate revealed the presence of labeled N-acetylgalactosamine 4,6-bissulfate at significant levels in the blood and cartilage, but at much lower levels in the liver. The cartilage component was highest in its rate of 35S uptake, suggesting that the blood component may derive at least in some part from the cartilage. Exposure of surviving cartilage slices to inorganic [35S]sulfate, followed by extraction of the slices with hot 50% ethanol yielded a number of radioactive compounds, of which three were characterized as UDP-N-acetylgalactosamine-4,6-[35S]bissulfate, N-acetylgalactosamine-1-phosphate 4,6-[35S]bissulfate and N-acetylgalactosamine 4,6-[35S]bissulfate. By subjecting the prelabeled tissue to chase incubation, it was possible to show that the UDP-N-acetylgalactosamine-4,6-bissulfate in the tissue disappeared with an approximate half-life of 10 min with a concomitant appearance in the medium of N-acetylgalactosamine 4,6-bissulfate and its 1-phosphate ester. These results suggest the occurrence in cartilage of an enzymatic system which is responsible for rapid turnover of UDP-N-acetylgalactosamine-4,6-bissulfate and possibly required for the rapid secretion of N-acetylgalactosamine 4,6-bissulfate into extracellular field.", "PMID": 952927}
{"id": "pubmed23n0032_319", "title": "The glutathione thiol-disulfide status in the sea urchin egg during fertilization and the first cell division cycle.", "content": "The intracellular levels of GSH, GSSG, and protein-glutathione disulfide (protein-SSG) have been measured in the eggs and developing embryos of the sea urchins Lytechinus pictus and Stronglyocentrotus purpuratus. Total cellular glutathione is maintained in a very highly reduced state during these initial stages of development. Thus for unfertilized eggs of L. pictus the results (mumol/g dry weight) were 11 +/- 1 for GSH, 0.02 +/- 0.01 for GSSG, and 0.07 +/- 0.02 for protein-SSG. No significant change in these values was observed upon fertilization of the eggs or during the first cell division cycle. The values obtained with S. purpuratus were somewhat greater, but were also found to exhibit no significant variations upon fertilization or cell division. These observations indicate that changes in the total cellular glutathione thiol-disulfide status are not involved in the control mechanisms which operate during fertilization of the first cell division cycle in the sea urchin egg.", "contents": "The glutathione thiol-disulfide status in the sea urchin egg during fertilization and the first cell division cycle. The intracellular levels of GSH, GSSG, and protein-glutathione disulfide (protein-SSG) have been measured in the eggs and developing embryos of the sea urchins Lytechinus pictus and Stronglyocentrotus purpuratus. Total cellular glutathione is maintained in a very highly reduced state during these initial stages of development. Thus for unfertilized eggs of L. pictus the results (mumol/g dry weight) were 11 +/- 1 for GSH, 0.02 +/- 0.01 for GSSG, and 0.07 +/- 0.02 for protein-SSG. No significant change in these values was observed upon fertilization of the eggs or during the first cell division cycle. The values obtained with S. purpuratus were somewhat greater, but were also found to exhibit no significant variations upon fertilization or cell division. These observations indicate that changes in the total cellular glutathione thiol-disulfide status are not involved in the control mechanisms which operate during fertilization of the first cell division cycle in the sea urchin egg.", "PMID": 952928}
{"id": "pubmed23n0032_320", "title": "Branched-chain amino acid oxidation by isolated rat tissue preparations.", "content": "Branched-chain amino acid transaminase activity, branced-chain alpha-keto acid dehydrogenase activity, and leucine oxidation were measured in homogenates and slices of several rat tissues. Transaminase activity was highest in heart, while dehydrogenase activity was highest in liver. Leucine oxidation in isolated tissues may be limited by either transaminase or dehydrogenase activity depending upon the relative activities of these two enzymes in the tissue. The results suggest that, as the load of branched-chain amino acids increases, the liver may become an increasingly important site for the degradation of branched-chain alpha-keto acids.", "contents": "Branched-chain amino acid oxidation by isolated rat tissue preparations. Branched-chain amino acid transaminase activity, branced-chain alpha-keto acid dehydrogenase activity, and leucine oxidation were measured in homogenates and slices of several rat tissues. Transaminase activity was highest in heart, while dehydrogenase activity was highest in liver. Leucine oxidation in isolated tissues may be limited by either transaminase or dehydrogenase activity depending upon the relative activities of these two enzymes in the tissue. The results suggest that, as the load of branched-chain amino acids increases, the liver may become an increasingly important site for the degradation of branched-chain alpha-keto acids.", "PMID": 952929}
{"id": "pubmed23n0032_321", "title": "A new agglutinating activity from wheat flour inhibited by tryptophan.", "content": "A new compound endowed with agglutinating activity, designated the flour agglutinin, was extracted from wheat flour with water and purified by gel filtration and ion-exchange chromatography. The haptenic inhibitors of the plant agglutinins do not affect flour agglutinin activity which, on the other hand, is inhibited by D- and L-tryptophan. Flour agglutinin has a molecular weight of about 5 - 10(4) as determined by gel filtration. It consists of a neutral heteropolysaccharide constituted of D-xylose and L-arabinose, and is homogeneous as judged by sedimentation analysis. Flour agglutinin activity is destroyed by treatment with Cellulase 2000 and periodate, but is not affected by alpha-amylase and proteolytic enzymes. Compared to germ agglutinin, flour agglutinin exhibits a peculiar range of cell specificity. It agglutinates several normal cell types, but has no effects on some neoplastic cells tested. Tryptic digestion of erythrocytes does not affect their susceptibility to flour agglutinin-induced agglutination.", "contents": "A new agglutinating activity from wheat flour inhibited by tryptophan. A new compound endowed with agglutinating activity, designated the flour agglutinin, was extracted from wheat flour with water and purified by gel filtration and ion-exchange chromatography. The haptenic inhibitors of the plant agglutinins do not affect flour agglutinin activity which, on the other hand, is inhibited by D- and L-tryptophan. Flour agglutinin has a molecular weight of about 5 - 10(4) as determined by gel filtration. It consists of a neutral heteropolysaccharide constituted of D-xylose and L-arabinose, and is homogeneous as judged by sedimentation analysis. Flour agglutinin activity is destroyed by treatment with Cellulase 2000 and periodate, but is not affected by alpha-amylase and proteolytic enzymes. Compared to germ agglutinin, flour agglutinin exhibits a peculiar range of cell specificity. It agglutinates several normal cell types, but has no effects on some neoplastic cells tested. Tryptic digestion of erythrocytes does not affect their susceptibility to flour agglutinin-induced agglutination.", "PMID": 952930}
{"id": "pubmed23n0032_322", "title": "Comparison of secreted and extracted forms of rat pituitary prolactin.", "content": "Prolactin secreted by rat anterior pituitary explants into organ culture medium was purified by salt fractionation and gel filtration. A yield of 22 mg/g was obtained, which clearly represented de novo synthesis and secretion of the hormone. Comparative characterization studies were performed on the secreted prolactin and pituitary extracted rat prolactin obtained from the National Institute of Arthritis, Metabolism and Digestive Disease. The biological and immunological activity estimates of both forms were comparable, although the specific activities of the secreted prolactin were somewhat lower than those of the pituitary prolactin. The secreted and extracted forms of prolactin appeared to be identical in primary structure as evidenced by similar amino acid compositions and identical NH2-terminal sequences. Circular dichroism spectra suggested that there may be differences in tertiary structure, since the positive tryptophan band at 292 nm, which eas observed with extracted hormone, was absent in the secreted prolactin.", "contents": "Comparison of secreted and extracted forms of rat pituitary prolactin. Prolactin secreted by rat anterior pituitary explants into organ culture medium was purified by salt fractionation and gel filtration. A yield of 22 mg/g was obtained, which clearly represented de novo synthesis and secretion of the hormone. Comparative characterization studies were performed on the secreted prolactin and pituitary extracted rat prolactin obtained from the National Institute of Arthritis, Metabolism and Digestive Disease. The biological and immunological activity estimates of both forms were comparable, although the specific activities of the secreted prolactin were somewhat lower than those of the pituitary prolactin. The secreted and extracted forms of prolactin appeared to be identical in primary structure as evidenced by similar amino acid compositions and identical NH2-terminal sequences. Circular dichroism spectra suggested that there may be differences in tertiary structure, since the positive tryptophan band at 292 nm, which eas observed with extracted hormone, was absent in the secreted prolactin.", "PMID": 952931}
{"id": "pubmed23n0032_323", "title": "Effects of prostaglandins and catecholamines on rat spleen adenylate cyclase in vitro.", "content": "The results reported here show some characteristics of adenylate cylase (EC 4.6.1.1) derived from homogenates of rat spleen, and describe the in vitro stimulation of this enzyme by prostaglandins, nucleotides, and F- under conditions where cyclic nucleotide degradative pathways are effectively inhibited. Particulate fractions from rat spleen homogenates contain high adenylate cyclase activities, and the highest specific activity is recovered in a particulate fraction prepared by low speed (1200 X g) centrifugation. Activity found in all particulate fractions is stimulated by fluoride, prostaglandins E1 and E2, catecholamines, and purine nucleotides. No stimulation is caused by prostaglandins F1 alpha and F2 alpha. Stimulation by prostaglandin E1 and E2 is augmented by GTP and other purine nucleotides, and stimulation by the combination of GTP and prostaglandin E1 is equal to that caused by optimal fluoride concentrations. Stimulation caused by L-isoproterenol is additive to that caused by GTP but is not increased by GTP.", "contents": "Effects of prostaglandins and catecholamines on rat spleen adenylate cyclase in vitro. The results reported here show some characteristics of adenylate cylase (EC 4.6.1.1) derived from homogenates of rat spleen, and describe the in vitro stimulation of this enzyme by prostaglandins, nucleotides, and F- under conditions where cyclic nucleotide degradative pathways are effectively inhibited. Particulate fractions from rat spleen homogenates contain high adenylate cyclase activities, and the highest specific activity is recovered in a particulate fraction prepared by low speed (1200 X g) centrifugation. Activity found in all particulate fractions is stimulated by fluoride, prostaglandins E1 and E2, catecholamines, and purine nucleotides. No stimulation is caused by prostaglandins F1 alpha and F2 alpha. Stimulation by prostaglandin E1 and E2 is augmented by GTP and other purine nucleotides, and stimulation by the combination of GTP and prostaglandin E1 is equal to that caused by optimal fluoride concentrations. Stimulation caused by L-isoproterenol is additive to that caused by GTP but is not increased by GTP.", "PMID": 952932}
{"id": "pubmed23n0032_324", "title": "Characterization and partial purification of a substance in the pineal gland which inhibits cell multiplication in vitro.", "content": "A substance which inhibits the in vitro multiplication of 3 cell strains, 37 RC, KB and NCTC clone 929, was characterized in the sheep pineal gland and partially purified using three successive chromatography techniques, respectively on Sephadex G-25, CM-cellulose and Biogel P 60 columns. The sheep cerebral cortex and liver also contain, but at much lower concentration than in the pineal, substance(s) that behave in different tests like the factor isolated from the pineal. The nature of the antimitotic substance from the pineal is as yet unknown. It is not destroyed by treatment with proteolytic enzymes, nor by boiling with 6 M HC1. It was established that it is different from the known antiblastic drugs such as Daunomycin and Methotrexate and from some active substances known to be present in the pineal, such as melatonin, secotonin and norepinephrine, which, in the same conditions, did not show any antimitotic activity. It was shown that when the concentration of the pineal factor in the culture medium was high enough (10 mug/ml), the inhibition of the KB cells multiplication was complete and irreversible. Microscopic examination of the treated cells showed that the morphological alteration was rapid (3--6 h) and deep, with shrinkage of both cytoplasm and nucleus, while with antiblastic drugs, morphological alteration proceeded slower (1--3 days) and appeared less pronounced.", "contents": "Characterization and partial purification of a substance in the pineal gland which inhibits cell multiplication in vitro. A substance which inhibits the in vitro multiplication of 3 cell strains, 37 RC, KB and NCTC clone 929, was characterized in the sheep pineal gland and partially purified using three successive chromatography techniques, respectively on Sephadex G-25, CM-cellulose and Biogel P 60 columns. The sheep cerebral cortex and liver also contain, but at much lower concentration than in the pineal, substance(s) that behave in different tests like the factor isolated from the pineal. The nature of the antimitotic substance from the pineal is as yet unknown. It is not destroyed by treatment with proteolytic enzymes, nor by boiling with 6 M HC1. It was established that it is different from the known antiblastic drugs such as Daunomycin and Methotrexate and from some active substances known to be present in the pineal, such as melatonin, secotonin and norepinephrine, which, in the same conditions, did not show any antimitotic activity. It was shown that when the concentration of the pineal factor in the culture medium was high enough (10 mug/ml), the inhibition of the KB cells multiplication was complete and irreversible. Microscopic examination of the treated cells showed that the morphological alteration was rapid (3--6 h) and deep, with shrinkage of both cytoplasm and nucleus, while with antiblastic drugs, morphological alteration proceeded slower (1--3 days) and appeared less pronounced.", "PMID": 952933}
{"id": "pubmed23n0032_325", "title": "Diffusion coefficient and molecular weight of type 5 adenovirus by photon-correlation spectroscopy.", "content": "The technique of photon-correlation spectroscopy (intensity fluctuation spectroscopy) is applied to light scatter from type 5 adenovirus undergoing Brownian motion in solution and the translation diffusion coefficient (D20,w) measured to be 0,367 +/- 0.003 Fick units. Using Svedberg's equation with previously determined parameters, a molecular weight of 165 - 10(6) +/- 5 - 10(6) is obtained.", "contents": "Diffusion coefficient and molecular weight of type 5 adenovirus by photon-correlation spectroscopy. The technique of photon-correlation spectroscopy (intensity fluctuation spectroscopy) is applied to light scatter from type 5 adenovirus undergoing Brownian motion in solution and the translation diffusion coefficient (D20,w) measured to be 0,367 +/- 0.003 Fick units. Using Svedberg's equation with previously determined parameters, a molecular weight of 165 - 10(6) +/- 5 - 10(6) is obtained.", "PMID": 952934}
{"id": "pubmed23n0032_326", "title": "A proton magnetic resonance study of water in human stratum corneum.", "content": "Proton magnetic resonance spectroscopy has been used to study the nature of water in human stratum corneum. For a single planar sheet of stratum corneum mounted at a specific orientation to the applied magnetic fild, three distinct absorptions may be seen having different chemical shifts and spin-lattice relaxation times (T1). All T1 values for these resonances are smaller than that for normal liquid water. One absorption is unusual in that the resonance position is dependent upon the orientation of the sample within the field.", "contents": "A proton magnetic resonance study of water in human stratum corneum. Proton magnetic resonance spectroscopy has been used to study the nature of water in human stratum corneum. For a single planar sheet of stratum corneum mounted at a specific orientation to the applied magnetic fild, three distinct absorptions may be seen having different chemical shifts and spin-lattice relaxation times (T1). All T1 values for these resonances are smaller than that for normal liquid water. One absorption is unusual in that the resonance position is dependent upon the orientation of the sample within the field.", "PMID": 952935}
{"id": "pubmed23n0032_327", "title": "Detection of stable isotopes of lithium or boron with the help of A (n,alpha) nuclear reaction. Application to the use of 6Li as a tracer for unidirectional flux measurements and to the microlocalization of lithium in animal histologic preparations.", "content": "In the particular case of boron and lithium we examine the possibilities of using stable isotopes for experiments of isotopic labelling and microlocalization, as no radioisotopes exist. The detection is made with the help of a specific nuclear reaction, using homogeneous detectors. The first experimental applications are given: transepithelial fluxes of lithium (frog skin) have shown Liefflux values larger than the influx ones. Detailed microlocalization of lithium have been made on histological preparations of mice having received lithium treatment: particularly important contents are found in the hypophysis, the salivary glands, the bladder, the kidney (especially the pelvis), the intestinal system and certain parts of the brain (particularly the hippocampus); the liver, however remains very poor in lithium. Physiological implications are examined.", "contents": "Detection of stable isotopes of lithium or boron with the help of A (n,alpha) nuclear reaction. Application to the use of 6Li as a tracer for unidirectional flux measurements and to the microlocalization of lithium in animal histologic preparations. In the particular case of boron and lithium we examine the possibilities of using stable isotopes for experiments of isotopic labelling and microlocalization, as no radioisotopes exist. The detection is made with the help of a specific nuclear reaction, using homogeneous detectors. The first experimental applications are given: transepithelial fluxes of lithium (frog skin) have shown Liefflux values larger than the influx ones. Detailed microlocalization of lithium have been made on histological preparations of mice having received lithium treatment: particularly important contents are found in the hypophysis, the salivary glands, the bladder, the kidney (especially the pelvis), the intestinal system and certain parts of the brain (particularly the hippocampus); the liver, however remains very poor in lithium. Physiological implications are examined.", "PMID": 952936}
{"id": "pubmed23n0032_328", "title": "The proteolytic nature of commercial samples of galactose oxidase. Purification of the enzyme by a simple affinity method.", "content": "Several commercially available samples of galactose oxidase (D-galactose: oxygen 6-oxidoreductase, EC 1.1.3.9) were found to contain high proteolytic activity on proteins such as fibrinogen, transferrin, albumin and casein. A simple, efficient method was devised for the purification of galactose oxidase which relies on the affinity of the enzyme for agarose (Sepharose 6B). The purified galactose oxidase was recovered in high yield free from proteolytic activity. The enzymic affinity for Sepharose and Sephadex was investigated to clarify the absorption mechanism.", "contents": "The proteolytic nature of commercial samples of galactose oxidase. Purification of the enzyme by a simple affinity method. Several commercially available samples of galactose oxidase (D-galactose: oxygen 6-oxidoreductase, EC 1.1.3.9) were found to contain high proteolytic activity on proteins such as fibrinogen, transferrin, albumin and casein. A simple, efficient method was devised for the purification of galactose oxidase which relies on the affinity of the enzyme for agarose (Sepharose 6B). The purified galactose oxidase was recovered in high yield free from proteolytic activity. The enzymic affinity for Sepharose and Sephadex was investigated to clarify the absorption mechanism.", "PMID": 952937}
{"id": "pubmed23n0032_329", "title": "The reaction of sulfhydryl reagents with bovine hepatic monoamine oxidase. Evidence for the presence of two cysteine residues essential for activity.", "content": "The bovine liver monoamine oxidase (EC 1.4.3.4) was found to be inactivated by various well-known sulfhydryl reagents like p-mercuribenzoate, methylmercuric iodide and 5,5'-dithiobis-(2-nitro benzoic acid). The present investigation shows that the inactivation of the enzyme results from reactions of these reagents with 2 out of 8 titratable sulfhydryl groups per 10(5) g of the enzyme. The substrate, benzylamine, and competitive inhibitors like benzaldehyde, p-nitrobenzaldehyde, benzyl alcohol protected the enzyme from inactivation by the mercurials or the Ellman reagent. The inactivation experiments with these sulfhydryl reagents, the protection experiments, and the kinetics as well as physicochemical observations suggest that there are only two cysteine residues that are required for activity of the enzyme. It is possible that these two residues may be active-center residues.", "contents": "The reaction of sulfhydryl reagents with bovine hepatic monoamine oxidase. Evidence for the presence of two cysteine residues essential for activity. The bovine liver monoamine oxidase (EC 1.4.3.4) was found to be inactivated by various well-known sulfhydryl reagents like p-mercuribenzoate, methylmercuric iodide and 5,5'-dithiobis-(2-nitro benzoic acid). The present investigation shows that the inactivation of the enzyme results from reactions of these reagents with 2 out of 8 titratable sulfhydryl groups per 10(5) g of the enzyme. The substrate, benzylamine, and competitive inhibitors like benzaldehyde, p-nitrobenzaldehyde, benzyl alcohol protected the enzyme from inactivation by the mercurials or the Ellman reagent. The inactivation experiments with these sulfhydryl reagents, the protection experiments, and the kinetics as well as physicochemical observations suggest that there are only two cysteine residues that are required for activity of the enzyme. It is possible that these two residues may be active-center residues.", "PMID": 952938}
{"id": "pubmed23n0032_330", "title": "Interaction of glutathione-insulin transhydrogenase (disulfide interchange enzyme) with phospholipids.", "content": "The effect of the addition of several phospholipids (lysophosphatidylcholine, alpha-lecithin, phosphatidylserine, phosphatidylethanolamine, lysophosphatidyl-ethanolamine, sphingomyelin, and disphosphatidylglycerol and phosphatidic acid) and related compounds (glycerophosphocholine, alpha- and beta-glycerophosphate, choline, serine, glycerol, dipalmitoylglycerol, and stearic acid) on the ability of purified (from beef pancreas) and microsomal (rat liver) glutathione-insulin transhydrogenase (Glutathione:protein-disulphide oxidoreductase, EC 1.8.4.2) to degrade insulin has been examined. With purified enzyme, except for phosphatidic acid and phosphatidylserine, all other phospholipids tested caused a slight activation at low concentration with phosphatidylethanolamine causing the highest activation. Lysophosphatidylcholine and phosphatidic acid are the only agents which cause inhibition of activity. The reaction rate as a function of concentration of inhibitor is hyperbolic for phosphatidic acid ([I]0.5 = 25 muM) and biphasic for lysophosphatidylcholine ([I]0.5 = 270 muM). Kinetic studies show that the two phospholipids are noncompetitive versus both substrates (insulin and GSH). Further, the structures of the phospholipids are quite different from the substrates and products of the reaction catalyzed by the enzyme. These dats, together with the data obtained with microsomes (see below), support the possibility that phospholipids, in particular lysolecithin and phosphatidic acid, might function by an interaction at an allosteric site or sites to bring about a conformational change in the enzyme. With a microsomal fraction, four phospholipids (lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylethanolamine, and phosphatidic acid) caused an increase in GSH-insulin transhydrogenase activity. At low concentration the addition of each of these phospholipids led to a 2.5-fold increase in GSH-insulin transhydrogenase activity. At higher concentration, lysophosphatidylcholine almost totally inhibited the microsomal GSH-insulin transhydrogenase activity, as it did with purified enzyme, while phosphatidic acid showed only a slight inhibition, in contrast to its effect on purified enzyme. With the microsomal fraction in which GSH-insulin transhydrogenase activity had been previously unmasked by Triton X-100 treatment, the addition of small amounts of lysophosphatidylcholine and phosphatidic acid produced, as expected, only slight increase in the transhydrogenase activity for both phospholipids; again, only lysophosphatidylcholine but not phosphatidic acid caused inhibition when higher levels were used. It is concluded that the four phospholipids and Triton X-100 increase the GSH-insulin transhydrogenase activity in the microsomes by unmasking the catalytic site without fully unmasking the allosteric site, the point of reaction with the phosphatidic acid.", "contents": "Interaction of glutathione-insulin transhydrogenase (disulfide interchange enzyme) with phospholipids. The effect of the addition of several phospholipids (lysophosphatidylcholine, alpha-lecithin, phosphatidylserine, phosphatidylethanolamine, lysophosphatidyl-ethanolamine, sphingomyelin, and disphosphatidylglycerol and phosphatidic acid) and related compounds (glycerophosphocholine, alpha- and beta-glycerophosphate, choline, serine, glycerol, dipalmitoylglycerol, and stearic acid) on the ability of purified (from beef pancreas) and microsomal (rat liver) glutathione-insulin transhydrogenase (Glutathione:protein-disulphide oxidoreductase, EC 1.8.4.2) to degrade insulin has been examined. With purified enzyme, except for phosphatidic acid and phosphatidylserine, all other phospholipids tested caused a slight activation at low concentration with phosphatidylethanolamine causing the highest activation. Lysophosphatidylcholine and phosphatidic acid are the only agents which cause inhibition of activity. The reaction rate as a function of concentration of inhibitor is hyperbolic for phosphatidic acid ([I]0.5 = 25 muM) and biphasic for lysophosphatidylcholine ([I]0.5 = 270 muM). Kinetic studies show that the two phospholipids are noncompetitive versus both substrates (insulin and GSH). Further, the structures of the phospholipids are quite different from the substrates and products of the reaction catalyzed by the enzyme. These dats, together with the data obtained with microsomes (see below), support the possibility that phospholipids, in particular lysolecithin and phosphatidic acid, might function by an interaction at an allosteric site or sites to bring about a conformational change in the enzyme. With a microsomal fraction, four phospholipids (lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylethanolamine, and phosphatidic acid) caused an increase in GSH-insulin transhydrogenase activity. At low concentration the addition of each of these phospholipids led to a 2.5-fold increase in GSH-insulin transhydrogenase activity. At higher concentration, lysophosphatidylcholine almost totally inhibited the microsomal GSH-insulin transhydrogenase activity, as it did with purified enzyme, while phosphatidic acid showed only a slight inhibition, in contrast to its effect on purified enzyme. With the microsomal fraction in which GSH-insulin transhydrogenase activity had been previously unmasked by Triton X-100 treatment, the addition of small amounts of lysophosphatidylcholine and phosphatidic acid produced, as expected, only slight increase in the transhydrogenase activity for both phospholipids; again, only lysophosphatidylcholine but not phosphatidic acid caused inhibition when higher levels were used. It is concluded that the four phospholipids and Triton X-100 increase the GSH-insulin transhydrogenase activity in the microsomes by unmasking the catalytic site without fully unmasking the allosteric site, the point of reaction with the phosphatidic acid.", "PMID": 952939}
{"id": "pubmed23n0032_331", "title": "The further heterogeneity of creatine kinase. Presence of isoenzymes of cathodic mobility in rat tissues.", "content": "Supernatant fractions from rat tissue homogenates were examined for creatine kinase (EC 2.7.3.2) isoenzymes by agarose gel electrophoresis at pH 8.6. The distribution of the various isoenzymes, and their relative contributions to the total tissue creatine kinase, is stated. A previously unreported isoenzyme associated with brain and kidney preparations is described. It is a minor contributor to brain creatine-kinase activity but is the major isoenzyme for kidney. The presence of an additional isoenzyme for cardiac tissue was confirmed; this isoenzyme exhibits similar levels of activity to that of the MB isoenzyme.", "contents": "The further heterogeneity of creatine kinase. Presence of isoenzymes of cathodic mobility in rat tissues. Supernatant fractions from rat tissue homogenates were examined for creatine kinase (EC 2.7.3.2) isoenzymes by agarose gel electrophoresis at pH 8.6. The distribution of the various isoenzymes, and their relative contributions to the total tissue creatine kinase, is stated. A previously unreported isoenzyme associated with brain and kidney preparations is described. It is a minor contributor to brain creatine-kinase activity but is the major isoenzyme for kidney. The presence of an additional isoenzyme for cardiac tissue was confirmed; this isoenzyme exhibits similar levels of activity to that of the MB isoenzyme.", "PMID": 952940}
{"id": "pubmed23n0032_332", "title": "An essential tryptophan in the active site of phospholipase A2 from the venom of Bitis gabonica.", "content": "The role of tryptophan in phospholipase A2 (EC 3.1.1.4) from the venom of the gaboon viper, Bitis gabonica, has been investigated. Modification of the enzyme with N-bromosuccinimide and 2-nitrophenylsulfenylchloride showed that the two tryptophan residues in the enzyme, viz. Trp-28 and Trp-59, differ in reactivity towards the reagents. Only Trp-28 reacted with N-bromosuccinimide while a preferential reaction occurred between Trp-59 and 2-nitrophenyl-sulfenylchloride. In each case it was found that loss of enzyme activity was specifically correlated with modification of TRP-28. CD spectra indicated that neither the local nor the gross conformation of the enzyme was altered by modification of Trp-28 and it was therefore concluded that Trp-28 is crucial for enzyme activity. The active enzyme was protected against N-bromosuccinimide inactivation by micellar concentrations of substrate or substrate analogue, suggesting that Trp-28 is involved in substrate binding.", "contents": "An essential tryptophan in the active site of phospholipase A2 from the venom of Bitis gabonica. The role of tryptophan in phospholipase A2 (EC 3.1.1.4) from the venom of the gaboon viper, Bitis gabonica, has been investigated. Modification of the enzyme with N-bromosuccinimide and 2-nitrophenylsulfenylchloride showed that the two tryptophan residues in the enzyme, viz. Trp-28 and Trp-59, differ in reactivity towards the reagents. Only Trp-28 reacted with N-bromosuccinimide while a preferential reaction occurred between Trp-59 and 2-nitrophenyl-sulfenylchloride. In each case it was found that loss of enzyme activity was specifically correlated with modification of TRP-28. CD spectra indicated that neither the local nor the gross conformation of the enzyme was altered by modification of Trp-28 and it was therefore concluded that Trp-28 is crucial for enzyme activity. The active enzyme was protected against N-bromosuccinimide inactivation by micellar concentrations of substrate or substrate analogue, suggesting that Trp-28 is involved in substrate binding.", "PMID": 952941}
{"id": "pubmed23n0032_333", "title": "Differences in subunit activities in acetylcholinesterase as possible cause for apparent deviation from normal Michaelis-Menten kinetics.", "content": "1. Form Gp of acetylcholinesterase (EC 3.1.1.7) from electric eel gave curved Lineweaver-Burk plots with acetylcholine. The Hill coefficients were 0.50-0.55 at low ionic strength and increased to 0.93 with increasing ionic strength. In presence of atropine or hexamethonium normal Michaelis-Menten kinetics were observed. 2. Inhibition of the enzyme by iPr2P-F was biphasic. One half of the total enzyme activity decreased at a faster rate than the other half. With [3H]iPr2P-F, the extent of labelling was determined for the light and heavy subunits. At low [3H]iPr2P-F concentration only the light subunit was phosphorylated. Higher [3H]iPr2P-F concentrations and prolonged treatment increased the amount of label in the heavy subunit. 3. From these data it is concluded that the two subunits are labelled at different rates indicating different reactivity towards iPr2P-F as well as towards acetylcholine. These data might account for the apparent non-Michaelis-Menten type kinetics obtained at low ionic strength.", "contents": "Differences in subunit activities in acetylcholinesterase as possible cause for apparent deviation from normal Michaelis-Menten kinetics. 1. Form Gp of acetylcholinesterase (EC 3.1.1.7) from electric eel gave curved Lineweaver-Burk plots with acetylcholine. The Hill coefficients were 0.50-0.55 at low ionic strength and increased to 0.93 with increasing ionic strength. In presence of atropine or hexamethonium normal Michaelis-Menten kinetics were observed. 2. Inhibition of the enzyme by iPr2P-F was biphasic. One half of the total enzyme activity decreased at a faster rate than the other half. With [3H]iPr2P-F, the extent of labelling was determined for the light and heavy subunits. At low [3H]iPr2P-F concentration only the light subunit was phosphorylated. Higher [3H]iPr2P-F concentrations and prolonged treatment increased the amount of label in the heavy subunit. 3. From these data it is concluded that the two subunits are labelled at different rates indicating different reactivity towards iPr2P-F as well as towards acetylcholine. These data might account for the apparent non-Michaelis-Menten type kinetics obtained at low ionic strength.", "PMID": 952942}
{"id": "pubmed23n0032_334", "title": "Further characterization of subunit III of bovine procarboxypeptidase A-S6 as a non activatable zymogen.", "content": "The homology of Subunit III of bovine procarboxypeptidase A-S6 (EC 3.4.12.-) with Subunit II (bovine chymotrypsinogen C) of the same complex, already reported in a previous publication (Puigserver, A. and Desnuelle, P. (1975) Proc. Natl. Acad. Sci. U.S. 72, 2442-2445) has been extended to the position of the single methionine of the chains. The sequence linked by 4 disulfide bridges out of 5 are also probably homologous in the 2 proteins. The last bridge is displaced in Subunit III as a consequence of the deletion of the N-terminal half-cystine. Subunit II, which is not activatable by trypsin, due to the loss of essential residues in the N-terminal region, has conserved a weakly functional active site reacting with concentrated diisopropylfluorophosphate at exactly the same rate as that of Subunit II.", "contents": "Further characterization of subunit III of bovine procarboxypeptidase A-S6 as a non activatable zymogen. The homology of Subunit III of bovine procarboxypeptidase A-S6 (EC 3.4.12.-) with Subunit II (bovine chymotrypsinogen C) of the same complex, already reported in a previous publication (Puigserver, A. and Desnuelle, P. (1975) Proc. Natl. Acad. Sci. U.S. 72, 2442-2445) has been extended to the position of the single methionine of the chains. The sequence linked by 4 disulfide bridges out of 5 are also probably homologous in the 2 proteins. The last bridge is displaced in Subunit III as a consequence of the deletion of the N-terminal half-cystine. Subunit II, which is not activatable by trypsin, due to the loss of essential residues in the N-terminal region, has conserved a weakly functional active site reacting with concentrated diisopropylfluorophosphate at exactly the same rate as that of Subunit II.", "PMID": 952943}
{"id": "pubmed23n0032_335", "title": "A sensitive isotopic assay method for S-adenosylhomocysteine hydrolase. Some properties of the enzyme from rat liver.", "content": "A rapid and sensitive isotopic method is presented for the assay of S-adenosylhomocysteine hydrolase (EC 3.3.1.1) activity, based on the formation of radioactive S-adenosylhomocysteine labelled in the adenosine portion. The radioactive product is separated either by low-voltage paper electrophoresis or by using phosphocellulose ion-exchange paper. Some kinetic properties of the enzyme from rat liver have shown to be clearly different from those reported earlier for this enzyme. The use of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent inhibitor  of adenosine deaminase, makes it possible to measure the S-adenosylhomocysteine hydrolase activity in tissues with a high adenosine deaminase activity, e.g. in intestinal mucosa.", "contents": "A sensitive isotopic assay method for S-adenosylhomocysteine hydrolase. Some properties of the enzyme from rat liver. A rapid and sensitive isotopic method is presented for the assay of S-adenosylhomocysteine hydrolase (EC 3.3.1.1) activity, based on the formation of radioactive S-adenosylhomocysteine labelled in the adenosine portion. The radioactive product is separated either by low-voltage paper electrophoresis or by using phosphocellulose ion-exchange paper. Some kinetic properties of the enzyme from rat liver have shown to be clearly different from those reported earlier for this enzyme. The use of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent inhibitor  of adenosine deaminase, makes it possible to measure the S-adenosylhomocysteine hydrolase activity in tissues with a high adenosine deaminase activity, e.g. in intestinal mucosa.", "PMID": 952944}
{"id": "pubmed23n0032_336", "title": "Partial purification and properties of N-acetylhistamine deacetylase.", "content": "The enzyme catalyzing the deacetylaction of N-acetylhistamine was partially purified about 160-fold from rat liver extract and its properties were investigated. The purification procedure included DEAE-cellulose chromatography, precipitation with ammonium sulfate and DEAE-cellulose rechromatography. The enzyme contains a labile -SH group that is essential for its activity. Mn2+ and Co2+ enhanced the deacetylation reaction at low concentration. The molecular weight of the deacetylase was estimated to be about 70 000 from gel-filtration. Among various acetyl derivatives tested so far, N-acetylhistamine and to a lesser extent N-acetyltyramine served as the substrates. The Km value was 0.3 mM at the optimum pH 8.0 for N-acetylhistamine. Diphenhysramine, an antihistaminergic agent, inhibited the deacetylation remarkably.", "contents": "Partial purification and properties of N-acetylhistamine deacetylase. The enzyme catalyzing the deacetylaction of N-acetylhistamine was partially purified about 160-fold from rat liver extract and its properties were investigated. The purification procedure included DEAE-cellulose chromatography, precipitation with ammonium sulfate and DEAE-cellulose rechromatography. The enzyme contains a labile -SH group that is essential for its activity. Mn2+ and Co2+ enhanced the deacetylation reaction at low concentration. The molecular weight of the deacetylase was estimated to be about 70 000 from gel-filtration. Among various acetyl derivatives tested so far, N-acetylhistamine and to a lesser extent N-acetyltyramine served as the substrates. The Km value was 0.3 mM at the optimum pH 8.0 for N-acetylhistamine. Diphenhysramine, an antihistaminergic agent, inhibited the deacetylation remarkably.", "PMID": 952945}
{"id": "pubmed23n0032_337", "title": "The regulation of mouse liver ornithine decarboxylase by metabolites.", "content": "The enzyme ornithine decarboxylase (L-Ornithine carboxy-lyase, EC 4.1.1.17), has been partially purified from the livers of mice subjected to partial hepatectomy (6-8 h previously). Mouse liver ornithine decarboxylase requires pyridoxal phosphate, and dithiothreitol for maximal activity. The enzyme has a pH optimum of 7.3, it is inhibited in the presence of 0.3 M phosphate, glycine, Tricine and Tris. It shows no dependence on metal ions and is inhibited by high salt concentrations, particularly ammonium salts. The kinetics of the enzyme have been studied with putrescine (and analogs), spermidine and spermine, in the presence of both high and low levels of pyridoxal phosphate. High concentrations of pyridoxal phosphate inhibit the enzyme. The enzyme is also inhibited by low concentrations of putrescine (1 mM). As the concentration of putrescine increased to 10 mM, non-competitive inhibition was observed, this could be reversed by addition of higher levels of pyridoxal phosphate. Spermidine and spermine inhibit (noncompetitively) only at high concentrations (10 mM). Ornithine inhibits at high concentrations (2 mM). Spectral studies have shown that the observed kinetics of competitive inhibition at low concentrations of polyamine changing to noncompetitive inhibition at high polyamine concentrations are due to competition between enzyme and substrate (or inhibitor) for free (non-enzyme bound) pyridoxal phosphate. Noncompetitive inhibition arises through the formation of transient Schiff base complexes between amines and free pyridoxal phosphate. It also appears that the binding of substrate to the active site takes place through Schiff base formation with enzyme bound pyridoxal phosphate.", "contents": "The regulation of mouse liver ornithine decarboxylase by metabolites. The enzyme ornithine decarboxylase (L-Ornithine carboxy-lyase, EC 4.1.1.17), has been partially purified from the livers of mice subjected to partial hepatectomy (6-8 h previously). Mouse liver ornithine decarboxylase requires pyridoxal phosphate, and dithiothreitol for maximal activity. The enzyme has a pH optimum of 7.3, it is inhibited in the presence of 0.3 M phosphate, glycine, Tricine and Tris. It shows no dependence on metal ions and is inhibited by high salt concentrations, particularly ammonium salts. The kinetics of the enzyme have been studied with putrescine (and analogs), spermidine and spermine, in the presence of both high and low levels of pyridoxal phosphate. High concentrations of pyridoxal phosphate inhibit the enzyme. The enzyme is also inhibited by low concentrations of putrescine (1 mM). As the concentration of putrescine increased to 10 mM, non-competitive inhibition was observed, this could be reversed by addition of higher levels of pyridoxal phosphate. Spermidine and spermine inhibit (noncompetitively) only at high concentrations (10 mM). Ornithine inhibits at high concentrations (2 mM). Spectral studies have shown that the observed kinetics of competitive inhibition at low concentrations of polyamine changing to noncompetitive inhibition at high polyamine concentrations are due to competition between enzyme and substrate (or inhibitor) for free (non-enzyme bound) pyridoxal phosphate. Noncompetitive inhibition arises through the formation of transient Schiff base complexes between amines and free pyridoxal phosphate. It also appears that the binding of substrate to the active site takes place through Schiff base formation with enzyme bound pyridoxal phosphate.", "PMID": 952946}
{"id": "pubmed23n0032_338", "title": "The structure of human thrombin in relation to autolytic degradation.", "content": "Human thrombin was obtained by activation of human prothrombin with venom of the Australian Taipan (Oxyuranus scutellatus scutellatus). This thrombin was precipitated with ammonium sulphate (75% saturation) and subsequently purified by gel-filtration (Sephadex G-75), ion-exchange (CM-Sephadex C-50) and affinity (aminobenzamidine-CH-Sepharose) chromatography. The final preparation (affinity thrombin) had a specific activity of 2340 Iowa units per absorbance unit (A1cm280). Thrombin proteins focused between 5 and 7, while prothrombin proteins focused to pH values less than 5. SDS-acrylamide gel electrophoresis indicated molecular weights of greater than 70 000 for prothrombin and 39 000, 28 000, 25 000-23 000 and 15 000-13 000 for affinity thrombin proteins. The 39 000-dalton species predominated (greater than 90%) when the enzyme was inhibited with phenylmethanesulphonyl fluoride prior to dialysis for SDS electrophoresis. Lack of such inhibition reduced the amount of the 39 000-dalton species to less than 60% with concomitant increase of the smaller species. Peptide mapping studies indicated that the smaller species were structurally related to the 39 000-dalton species. The amino acid compositions of the histidine and/or tyrosine containing peptides indicated a high degree of homology with bovine thrombin. It has been established that human thrombin can exist in at least two secondary structural forms, of different molecular weights, probably due to autolytic degradation of the largest (39 000-dalton) form.", "contents": "The structure of human thrombin in relation to autolytic degradation. Human thrombin was obtained by activation of human prothrombin with venom of the Australian Taipan (Oxyuranus scutellatus scutellatus). This thrombin was precipitated with ammonium sulphate (75% saturation) and subsequently purified by gel-filtration (Sephadex G-75), ion-exchange (CM-Sephadex C-50) and affinity (aminobenzamidine-CH-Sepharose) chromatography. The final preparation (affinity thrombin) had a specific activity of 2340 Iowa units per absorbance unit (A1cm280). Thrombin proteins focused between 5 and 7, while prothrombin proteins focused to pH values less than 5. SDS-acrylamide gel electrophoresis indicated molecular weights of greater than 70 000 for prothrombin and 39 000, 28 000, 25 000-23 000 and 15 000-13 000 for affinity thrombin proteins. The 39 000-dalton species predominated (greater than 90%) when the enzyme was inhibited with phenylmethanesulphonyl fluoride prior to dialysis for SDS electrophoresis. Lack of such inhibition reduced the amount of the 39 000-dalton species to less than 60% with concomitant increase of the smaller species. Peptide mapping studies indicated that the smaller species were structurally related to the 39 000-dalton species. The amino acid compositions of the histidine and/or tyrosine containing peptides indicated a high degree of homology with bovine thrombin. It has been established that human thrombin can exist in at least two secondary structural forms, of different molecular weights, probably due to autolytic degradation of the largest (39 000-dalton) form.", "PMID": 952947}
{"id": "pubmed23n0032_339", "title": "Urea-extractable protein from human epidermis.", "content": "The major protein fraction extracted from human epidermis by urea has the following characteristics: (1) A molecular weight of approximately 600 000. (2) A copper content of 0.03% giving a minimum molecular weight of 212 000. (3) A non-protein moiety indicated by (a) protein/dry weight ratio (b) anomalous ultraviolet absorption spectrum (c) high-voltage electrophoresis after mild alkaline or acid treatment (d) an excess of imidazole groups by Pauly reaction compared with the number of histidine residues by amino acid analysis. The non-protein component is not urocanic acid or RNA as has been reported (Bernstein, I.A. (1970), J. Soc. Cosmet, Chem. 21, 583-594 and Sibrack, L. A., Gray, R. H. and Bernstein, I. A. (1974), J. Invest. Dermatol. 62, 394-405). (4) The presence of interchain disulphide linkages indicated by polyacrylamide gel electrophoresis in sodium dodecyl sulphate with and without beta-mercaptoethanol. (5) Polyacrylamide gel electrophoresis in presence and absence of 6 M urea suggests that aggregates are broken down in urea.", "contents": "Urea-extractable protein from human epidermis. The major protein fraction extracted from human epidermis by urea has the following characteristics: (1) A molecular weight of approximately 600 000. (2) A copper content of 0.03% giving a minimum molecular weight of 212 000. (3) A non-protein moiety indicated by (a) protein/dry weight ratio (b) anomalous ultraviolet absorption spectrum (c) high-voltage electrophoresis after mild alkaline or acid treatment (d) an excess of imidazole groups by Pauly reaction compared with the number of histidine residues by amino acid analysis. The non-protein component is not urocanic acid or RNA as has been reported (Bernstein, I.A. (1970), J. Soc. Cosmet, Chem. 21, 583-594 and Sibrack, L. A., Gray, R. H. and Bernstein, I. A. (1974), J. Invest. Dermatol. 62, 394-405). (4) The presence of interchain disulphide linkages indicated by polyacrylamide gel electrophoresis in sodium dodecyl sulphate with and without beta-mercaptoethanol. (5) Polyacrylamide gel electrophoresis in presence and absence of 6 M urea suggests that aggregates are broken down in urea.", "PMID": 952948}
{"id": "pubmed23n0032_340", "title": "Interaction between proteins and detergents which contain a hydrocarbon chain longer than 16 carbon atoms. III. Competitive inhibition of trypsin by cetyldimethylbenzylammonium chloride.", "content": "Tryptic activity was competitively inhibited by cationic detergents which contain a cetyl group or longer hydrocarbon chains. Since the cetyl group is much longer than the side chains of lysine or arginine residues of substrates for trypsin, the nature of inhibition by cetyldimethylbenzylammonium chloride was examined using Na-benzoyl-L-arginine-p-nitroanilide as substrate and compared to that by butylamine. The inhibition by cetyldimethylbenzylammonium chloride occurred instantaneously and was completely reversible. The inhibitory effect of cetyldimethylbenzylammonium chloride was strongly dependent on both pH and salt concentration, contrasting with inhibition by butylamine which was relatively indifferent to these changes. The Ki value of cetyldimethylbenzylammonium chloride at pH 7.5 and 25 degrees C was calculated to be 2.0 +/- 0.3 mM, which is equal to that of butylamine within experimental errors. The standard entropy change of binding of cetyldimethylbenzylammonium chloride (44 +/- 2 cal/mol/degree) was much larger than for butylamine, indicating the formation of an efficient hydrophobic bond between the cetyl group and the enzyme.", "contents": "Interaction between proteins and detergents which contain a hydrocarbon chain longer than 16 carbon atoms. III. Competitive inhibition of trypsin by cetyldimethylbenzylammonium chloride. Tryptic activity was competitively inhibited by cationic detergents which contain a cetyl group or longer hydrocarbon chains. Since the cetyl group is much longer than the side chains of lysine or arginine residues of substrates for trypsin, the nature of inhibition by cetyldimethylbenzylammonium chloride was examined using Na-benzoyl-L-arginine-p-nitroanilide as substrate and compared to that by butylamine. The inhibition by cetyldimethylbenzylammonium chloride occurred instantaneously and was completely reversible. The inhibitory effect of cetyldimethylbenzylammonium chloride was strongly dependent on both pH and salt concentration, contrasting with inhibition by butylamine which was relatively indifferent to these changes. The Ki value of cetyldimethylbenzylammonium chloride at pH 7.5 and 25 degrees C was calculated to be 2.0 +/- 0.3 mM, which is equal to that of butylamine within experimental errors. The standard entropy change of binding of cetyldimethylbenzylammonium chloride (44 +/- 2 cal/mol/degree) was much larger than for butylamine, indicating the formation of an efficient hydrophobic bond between the cetyl group and the enzyme.", "PMID": 952949}
{"id": "pubmed23n0032_341", "title": "The protein composition of bovine myelin-free axons.", "content": "The proteins of axons prepared from myelinated axons and isolated as myelin-free entities were separated by sodium dodecyl sulfate polyacrylamide electrophoresis and found to consist of more than 10 different molecular weight species. The molecular weights range from 13 000 to over 200 000 with a prominent protein of molecular weight 47 000. The amino acid composition of the seven major proteins showed that the protein with a molecular weigt of 47 000 is distinct from all the other proteins analyzed. A group of three low molecular weight proteins have amino acid compositions which are similar to each other as do a group of three high molecular weight proteins although the two groups are distinctly different from each other and the major axonal protein. Histones, DNA, myelin basic protein and glycoprotein were absent from the proteins but neurotubule protein was present as indicated by cochicine binding activity in the axonal preparations. The cellular origin of these proteins and their relationship to other central nervous system proteins are discussed.", "contents": "The protein composition of bovine myelin-free axons. The proteins of axons prepared from myelinated axons and isolated as myelin-free entities were separated by sodium dodecyl sulfate polyacrylamide electrophoresis and found to consist of more than 10 different molecular weight species. The molecular weights range from 13 000 to over 200 000 with a prominent protein of molecular weight 47 000. The amino acid composition of the seven major proteins showed that the protein with a molecular weigt of 47 000 is distinct from all the other proteins analyzed. A group of three low molecular weight proteins have amino acid compositions which are similar to each other as do a group of three high molecular weight proteins although the two groups are distinctly different from each other and the major axonal protein. Histones, DNA, myelin basic protein and glycoprotein were absent from the proteins but neurotubule protein was present as indicated by cochicine binding activity in the axonal preparations. The cellular origin of these proteins and their relationship to other central nervous system proteins are discussed.", "PMID": 952950}
{"id": "pubmed23n0032_342", "title": "Structure of a light-adapting hormone from the shrimp, Pandalus borealis.", "content": "The structure of a light-adapting hormone of the shrimp, Pandalus borealis, has been determined. The hormone, which had been isolated from Pandalus eyestalks and which adapts the shrimp to brighter light conditions by causing the pigment in the distal retinal pigment cells of the eye to move into a more proximal position, is the peptide: Asn-Ser-Gly-Met-Ile-Asn-Ser-Ile-Leu-Gly-Ile-Pro-Arg-Val-Met-Thr-Glu-Ala-NH2. The structure was obtained by sequence analysis by the dansyl-Edman method of the intact hormone and of isolated tryptic and thermolytic peptides.", "contents": "Structure of a light-adapting hormone from the shrimp, Pandalus borealis. The structure of a light-adapting hormone of the shrimp, Pandalus borealis, has been determined. The hormone, which had been isolated from Pandalus eyestalks and which adapts the shrimp to brighter light conditions by causing the pigment in the distal retinal pigment cells of the eye to move into a more proximal position, is the peptide: Asn-Ser-Gly-Met-Ile-Asn-Ser-Ile-Leu-Gly-Ile-Pro-Arg-Val-Met-Thr-Glu-Ala-NH2. The structure was obtained by sequence analysis by the dansyl-Edman method of the intact hormone and of isolated tryptic and thermolytic peptides.", "PMID": 952951}
{"id": "pubmed23n0032_343", "title": "Structural requirements for steroid binding and quenching of albumin fluorescence in bovine plasma albumin.", "content": "1. Steroids interact with bovine plasma albumin at a binding region that involves tryptophanyl, tyrosyl, arginyl and lysyl residues. The function of the tryptophanyl residues is demonstrated by: (a) the decrease of albumin binding affinity after modification of one tryptophanyl with 2-nitrophenylsulfenyl chloride; (b) steroid quenching of albumin tryptophanyl fluorescence; and (c) steroid quenching of 1-anilinonaphth-alene-8-sulfonate fluorescence, when it is excited by energy transfer from excited tryptophanyls. The function of tyrosyl residues is demonstrated by the decrease of albumin binding affinity after nitration of 30% tyrosyls with tetranitromethane, or deprotonation of tyrosyls by variation of pH. The function of arginyl and lysyl residues is demonstrated by the decrease of binding affinity after modification of these residues with glyoxal, formaldehyde or acetic anhydride. The presence of both apolar (Trp, Tyr and Lys (deprotonated)) and polar (Arg and Lys(protonated)) residues at the steroid binding site fits in well with the site relative apolarity, when expressed on the Kosower scale (Kosower, E.M. (1958) J. Am. Chem. Soc. 80, 3253-3260). 2. The contribution of specific amino acid residues to steroid binding depends to some extent on the steroid structure, as exemplified by the quantitatively different role of arginyl (or lysyl) residues in albumin interaction with testosterone acetate and epitestosterone, respectively, or that of tyrosyl residues in albumin interaction with 11-deoxycorticosterone and epitestosterone, respectively. 3. The concerted action of polar and apolar amino acid residues is an essential requirement for steroid binding, since unfolding of albumin polypeptide chain by guanidine-HC1, urea, or by reduction of disulfide bridges with 2-mercaptoethanol, strongly decreases steroid binding to albumin while, conversely, reoxidation and refolding of the unfolded polypeptide chain restore albumin affinity for steroids. 4. Parallel determinations of steroid binding constants by equilibrium dialysis and fluorimetric titration, as well as the general pattern of the pH and temperature effects on steroid quenching of albumin fluorescence, confirm the validity of the fluorescence quenching titration as an effective method for measuring albumin-steroid molecular interactions.", "contents": "Structural requirements for steroid binding and quenching of albumin fluorescence in bovine plasma albumin. 1. Steroids interact with bovine plasma albumin at a binding region that involves tryptophanyl, tyrosyl, arginyl and lysyl residues. The function of the tryptophanyl residues is demonstrated by: (a) the decrease of albumin binding affinity after modification of one tryptophanyl with 2-nitrophenylsulfenyl chloride; (b) steroid quenching of albumin tryptophanyl fluorescence; and (c) steroid quenching of 1-anilinonaphth-alene-8-sulfonate fluorescence, when it is excited by energy transfer from excited tryptophanyls. The function of tyrosyl residues is demonstrated by the decrease of albumin binding affinity after nitration of 30% tyrosyls with tetranitromethane, or deprotonation of tyrosyls by variation of pH. The function of arginyl and lysyl residues is demonstrated by the decrease of binding affinity after modification of these residues with glyoxal, formaldehyde or acetic anhydride. The presence of both apolar (Trp, Tyr and Lys (deprotonated)) and polar (Arg and Lys(protonated)) residues at the steroid binding site fits in well with the site relative apolarity, when expressed on the Kosower scale (Kosower, E.M. (1958) J. Am. Chem. Soc. 80, 3253-3260). 2. The contribution of specific amino acid residues to steroid binding depends to some extent on the steroid structure, as exemplified by the quantitatively different role of arginyl (or lysyl) residues in albumin interaction with testosterone acetate and epitestosterone, respectively, or that of tyrosyl residues in albumin interaction with 11-deoxycorticosterone and epitestosterone, respectively. 3. The concerted action of polar and apolar amino acid residues is an essential requirement for steroid binding, since unfolding of albumin polypeptide chain by guanidine-HC1, urea, or by reduction of disulfide bridges with 2-mercaptoethanol, strongly decreases steroid binding to albumin while, conversely, reoxidation and refolding of the unfolded polypeptide chain restore albumin affinity for steroids. 4. Parallel determinations of steroid binding constants by equilibrium dialysis and fluorimetric titration, as well as the general pattern of the pH and temperature effects on steroid quenching of albumin fluorescence, confirm the validity of the fluorescence quenching titration as an effective method for measuring albumin-steroid molecular interactions.", "PMID": 952952}
{"id": "pubmed23n0032_344", "title": "Specific fluorescent derivatives of macromolecules. A fluorescence study of some specifically modified derivatives of chymotrypsin, trypsin and subtilisin.", "content": "The 5-dimethylaminonaphthalene-1-sulfonyl group was specifically introduced into the active site region of the serine proteinases: alpha-chymotrypsin, trypsin and subtilisin Carlsberg by the method of affinity-labeling. The resulting fluorescent derivatives were studied by a variety of fluorescence techniques and the results were correlated with structural data available on these enzymes from X-ray analysis. As model compounds for the Dns-proteinases, the absorption and fluorescence properties of Dns-amide and Dns-ethyl ester were studied in ethanol/water and p-dioxane/water mixtures. The fluorescence emission transtion energies and quantum yields were related to four commonly employed solvent-polarity scales. Best correlations for different solvents were obatined with the empirical \"Z\" and \"Y\" scales. From inspection of the fluorescence emission transition energies of the Dns group in the Dns-proteinases and comparision with the model compound studies it was possible to assign \"Z\" values for the apparent microenvironment polarities of the Dns group in the Dns-proteinases. The apparent polarities of the microenvironments of the Dns group in Dns-Ser 195-chymotrypsin (Dns-chymotrypsin (I)); (Dns-Phe-CH2)-His 57-chymotrypsin;  (Dns-Lys-CH2)-His 46-trypsin; and Dns-Ser 221--subtilisin Carlsberg (Dns-subtilisin (I)) are in the range of 89.5-92.5 on the \"Z\" scale. The apparent microenvironment polarity of the Dns group in Dns-Ser 183-trypsin (Dns-trypsin (I)) appears to be below 76.7 on the \"Z\" scale. The Dns group in Dns-chymotrypsin (I) and (Dns-Phe-CH2)-His 57-chymotrypsin appears to be rigidly bound as evaluated by fluorescence polarization studies. The effect of 2H2O on the fluorescence emission quantum yields of Dns-amide and Dns-ethyl ester was examined. In both cases the ratios of quantum yields in 2H2O:ethanol (8:2) to quantum yields in H2O:ethanol (8:2) was about 1.8. The 2H2O effect upon the fluorescence emission quantum yields of the Dns group has been used to investigate solvent accessibility of this chromophore in the Dns-proteinases. Acessibility studies using 2H2O are very promising and have some definite advantages over other existing methods. Energy transfer between the Trp residues and the bound Dns group was investigated in the Dns-proteinases. The mean transfer distance calculated from the observed transfer efficiencies are 18.1 A, 19.7 A and 18.4 A for (Dns-Phe-CH2)-His 57-chymotrypsin, Dns-chymotrypsin (I) and (Dns-Lys-CH2)-His 46-trypsin, respectivly. From models built using X-ray crystallographic coordinates for the protein atoms, the mean distance of separation between the Trp residues and the bound Dns group for the same set of conjugates ar 18.6 A, 17.5 A and 17.5 A, respectively. Considering the inherent difficulties in energy transfer studies, the results are in excellent agreement with the X-ray data.", "contents": "Specific fluorescent derivatives of macromolecules. A fluorescence study of some specifically modified derivatives of chymotrypsin, trypsin and subtilisin. The 5-dimethylaminonaphthalene-1-sulfonyl group was specifically introduced into the active site region of the serine proteinases: alpha-chymotrypsin, trypsin and subtilisin Carlsberg by the method of affinity-labeling. The resulting fluorescent derivatives were studied by a variety of fluorescence techniques and the results were correlated with structural data available on these enzymes from X-ray analysis. As model compounds for the Dns-proteinases, the absorption and fluorescence properties of Dns-amide and Dns-ethyl ester were studied in ethanol/water and p-dioxane/water mixtures. The fluorescence emission transtion energies and quantum yields were related to four commonly employed solvent-polarity scales. Best correlations for different solvents were obatined with the empirical \"Z\" and \"Y\" scales. From inspection of the fluorescence emission transition energies of the Dns group in the Dns-proteinases and comparision with the model compound studies it was possible to assign \"Z\" values for the apparent microenvironment polarities of the Dns group in the Dns-proteinases. The apparent polarities of the microenvironments of the Dns group in Dns-Ser 195-chymotrypsin (Dns-chymotrypsin (I)); (Dns-Phe-CH2)-His 57-chymotrypsin;  (Dns-Lys-CH2)-His 46-trypsin; and Dns-Ser 221--subtilisin Carlsberg (Dns-subtilisin (I)) are in the range of 89.5-92.5 on the \"Z\" scale. The apparent microenvironment polarity of the Dns group in Dns-Ser 183-trypsin (Dns-trypsin (I)) appears to be below 76.7 on the \"Z\" scale. The Dns group in Dns-chymotrypsin (I) and (Dns-Phe-CH2)-His 57-chymotrypsin appears to be rigidly bound as evaluated by fluorescence polarization studies. The effect of 2H2O on the fluorescence emission quantum yields of Dns-amide and Dns-ethyl ester was examined. In both cases the ratios of quantum yields in 2H2O:ethanol (8:2) to quantum yields in H2O:ethanol (8:2) was about 1.8. The 2H2O effect upon the fluorescence emission quantum yields of the Dns group has been used to investigate solvent accessibility of this chromophore in the Dns-proteinases. Acessibility studies using 2H2O are very promising and have some definite advantages over other existing methods. Energy transfer between the Trp residues and the bound Dns group was investigated in the Dns-proteinases. The mean transfer distance calculated from the observed transfer efficiencies are 18.1 A, 19.7 A and 18.4 A for (Dns-Phe-CH2)-His 57-chymotrypsin, Dns-chymotrypsin (I) and (Dns-Lys-CH2)-His 46-trypsin, respectivly. From models built using X-ray crystallographic coordinates for the protein atoms, the mean distance of separation between the Trp residues and the bound Dns group for the same set of conjugates ar 18.6 A, 17.5 A and 17.5 A, respectively. Considering the inherent difficulties in energy transfer studies, the results are in excellent agreement with the X-ray data.", "PMID": 952953}
{"id": "pubmed23n0032_345", "title": "Studies on pituitary prolactin. 39. Reaction of the ovine hormone with hydrogen peroxide.", "content": "Three methionine-modified derivatives of ovine prolactin have been prepared: two by oxidation of the methionines by H2O2 to sulfoxide (partial and complete), and the third by complete alkylation of the metionines with iodoacetic acid to the carboxymethyl sulfonium salts. The derivatives were characterized by exclusion chromatography, amino acid composition, circular dichroism spectra, relative rates of digestion by trypsin, and biological activity. Partially oxidized prolactin, having four of its seven methionines oxidized, was very similar to the native hormone. The unmodified methionines in partially oxidized prolactin were found to be the residues at positions 36, 81 and 132. The prolactin derivatives in which all the methionines had been oxidized, or alkylated, showed major changes in all parameters examined. In addition, circular dichroism spectra indicated that complete modification of all the methionines in prolactin exposes the normally buried tryptophans.", "contents": "Studies on pituitary prolactin. 39. Reaction of the ovine hormone with hydrogen peroxide. Three methionine-modified derivatives of ovine prolactin have been prepared: two by oxidation of the methionines by H2O2 to sulfoxide (partial and complete), and the third by complete alkylation of the metionines with iodoacetic acid to the carboxymethyl sulfonium salts. The derivatives were characterized by exclusion chromatography, amino acid composition, circular dichroism spectra, relative rates of digestion by trypsin, and biological activity. Partially oxidized prolactin, having four of its seven methionines oxidized, was very similar to the native hormone. The unmodified methionines in partially oxidized prolactin were found to be the residues at positions 36, 81 and 132. The prolactin derivatives in which all the methionines had been oxidized, or alkylated, showed major changes in all parameters examined. In addition, circular dichroism spectra indicated that complete modification of all the methionines in prolactin exposes the normally buried tryptophans.", "PMID": 952954}
{"id": "pubmed23n0032_346", "title": "Optical diffraction of tropoelastin and alpha-elastin coacervates.", "content": "Optical diffraction applied to micrographs of coacervated tropoelastin and alpha-elastin show an equatorial repeat around 50 A. This confirms a 50 A center-to-center distance of parallel aligned filaments to be a fundamental property of the tropoelastin and alpha-elastin coacervates. This periodicity is similar to that of mature cross-linked elastin. These results allow the conclusion that hydrophobic association is the predominant driving force for formation of filamentous elastin in vitro. It is suggested that the coacervate is a model for relaxed fibrous elastin.", "contents": "Optical diffraction of tropoelastin and alpha-elastin coacervates. Optical diffraction applied to micrographs of coacervated tropoelastin and alpha-elastin show an equatorial repeat around 50 A. This confirms a 50 A center-to-center distance of parallel aligned filaments to be a fundamental property of the tropoelastin and alpha-elastin coacervates. This periodicity is similar to that of mature cross-linked elastin. These results allow the conclusion that hydrophobic association is the predominant driving force for formation of filamentous elastin in vitro. It is suggested that the coacervate is a model for relaxed fibrous elastin.", "PMID": 952955}
{"id": "pubmed23n0032_347", "title": "Further studies on a highly purified glycoprotein from the intimal region of procine aorta.", "content": "Highly purified glycoprotein from the intimal region of porcine aorta was isolated with minor modifications of the procedure described previously. The molecular weight of the glycoprotein as determined by sedimentation equilibrium method either in presence of 0.1 M NaCl or 6 M guanidine-HCl containing beta-mercaptoethanol was 72 000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native glycoprotein and its S-carboxyamidomethyl derivative at different acrylamide concentrations showed no difference in the molecular weight indicating the absence of subunits. Attempts to determine the identity of the amino-terminal acid by a dansylation technique indicated that the amino group is not free. The carboxy-terminal amino acid was found to be serine after treatment of the glycoprotein with carboxypeptidase A. The glycoprotein did not contain an alkali-labile (O-glycosidic) carbohydrate-protein linkage as tested by the beta-elimination reaction. The release of monosaccharides from the glycoprotein as a function of time was studied employing mild acid hydrolysis (0.5 M HCl, 80 degrees C) and also by the use of neuraminidase, alpha-D-and beta-D-glucosidases and beta-D-N-acetylglucosaminidase. From the observations of the release of monosaccharides and analogy with standard features determined by other investigators on soluble aortic glycoproteins, a prediction has been made as to the general features of the carbohydrate moiety of the glycoprotein.", "contents": "Further studies on a highly purified glycoprotein from the intimal region of procine aorta. Highly purified glycoprotein from the intimal region of porcine aorta was isolated with minor modifications of the procedure described previously. The molecular weight of the glycoprotein as determined by sedimentation equilibrium method either in presence of 0.1 M NaCl or 6 M guanidine-HCl containing beta-mercaptoethanol was 72 000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native glycoprotein and its S-carboxyamidomethyl derivative at different acrylamide concentrations showed no difference in the molecular weight indicating the absence of subunits. Attempts to determine the identity of the amino-terminal acid by a dansylation technique indicated that the amino group is not free. The carboxy-terminal amino acid was found to be serine after treatment of the glycoprotein with carboxypeptidase A. The glycoprotein did not contain an alkali-labile (O-glycosidic) carbohydrate-protein linkage as tested by the beta-elimination reaction. The release of monosaccharides from the glycoprotein as a function of time was studied employing mild acid hydrolysis (0.5 M HCl, 80 degrees C) and also by the use of neuraminidase, alpha-D-and beta-D-glucosidases and beta-D-N-acetylglucosaminidase. From the observations of the release of monosaccharides and analogy with standard features determined by other investigators on soluble aortic glycoproteins, a prediction has been made as to the general features of the carbohydrate moiety of the glycoprotein.", "PMID": 952956}
{"id": "pubmed23n0032_348", "title": "Investigations on the primary structure of human plasminogen. Further evidence for sequence homology.", "content": "NH2-Terminal sequences were determined by the automated Edman method in four fragments which were isolated from a mixture of fragments obtained by CNBr cleavage of human plasminogen. One of the fragments whose sequence was determined over the first 31 residues shows sequence homologies with the fragment that forms the linkage between the plasmin chains and also with the non-thrombin part of prothrombin.", "contents": "Investigations on the primary structure of human plasminogen. Further evidence for sequence homology. NH2-Terminal sequences were determined by the automated Edman method in four fragments which were isolated from a mixture of fragments obtained by CNBr cleavage of human plasminogen. One of the fragments whose sequence was determined over the first 31 residues shows sequence homologies with the fragment that forms the linkage between the plasmin chains and also with the non-thrombin part of prothrombin.", "PMID": 952957}
{"id": "pubmed23n0032_349", "title": "Comparison of the amino acid sequence of pig heart myoglobin with other ungulate myoglobins.", "content": "The primary structure of pig heart myoglobin has been established by study of the tryptic peptides of whole globin and by analysis of the fragments obtained by CNBr cleavage. Thermolysin and chymotrypsin digestion were used to determine the sequence of the M fragment (56-131). Automatic Edman degradation of whole globin and of the M fragment completed the sequence of pig myoglobin. Comparison with other ungulates shows that pig myoglobin is far from other artiodactyls previously studied (ox and sheep) and close to the eutherian ancestral chain.", "contents": "Comparison of the amino acid sequence of pig heart myoglobin with other ungulate myoglobins. The primary structure of pig heart myoglobin has been established by study of the tryptic peptides of whole globin and by analysis of the fragments obtained by CNBr cleavage. Thermolysin and chymotrypsin digestion were used to determine the sequence of the M fragment (56-131). Automatic Edman degradation of whole globin and of the M fragment completed the sequence of pig myoglobin. Comparison with other ungulates shows that pig myoglobin is far from other artiodactyls previously studied (ox and sheep) and close to the eutherian ancestral chain.", "PMID": 952959}
{"id": "pubmed23n0032_350", "title": "Characterization of the hemoglobin of Biomphalaria glabrata as a glycoprotein.", "content": "1. The hemoglobin of Biomphalaria glabrata is a glycoprotein which contains 3% sugars. 2. The sugar moiety is composed of 2 mol of hexose and one mol of hexosamine per mol of monomer. 3. The hexosamine in the molecule is glucosamine. 4. Mannose, galactose and fucose are present in the molecule in a ratio of 2:1:1. 5. No sialic and uronic acids were detected in this hemoglobin.", "contents": "Characterization of the hemoglobin of Biomphalaria glabrata as a glycoprotein. 1. The hemoglobin of Biomphalaria glabrata is a glycoprotein which contains 3% sugars. 2. The sugar moiety is composed of 2 mol of hexose and one mol of hexosamine per mol of monomer. 3. The hexosamine in the molecule is glucosamine. 4. Mannose, galactose and fucose are present in the molecule in a ratio of 2:1:1. 5. No sialic and uronic acids were detected in this hemoglobin.", "PMID": 952961}
{"id": "pubmed23n0032_351", "title": "A complex-forming glycoprotein heterogeneous in charge and present in human plasma, urine, and cerebrospinal fluid.", "content": "A glycoprotein from the urine of one healthy individual was purified by ultrafiltration, ion-exchange chromatography, gel chromatography and immunosorption. The protein contained only one polypeptide chain with an approximate molecular weight of 31 000 and was associated with a brown colour which did not disappear even after total reduction and alkylation of the protein followed by dialysis in 6 M guanidine hydrochloride. The protein appeared homogeneous on sodium dodecyl sulphate-polyacrylamide electrophoresis and gel chromatography. It had only one N-terminal amino acid sequence, Gly-Pro, and gave only one precipitate with a polyvalent antiserum but was found to be very heterorgeneous on agarose gel electrophoresis and on isoelectric focusing. Desialylation of the protein failed to alter this heterogeneity. An electroimmunoassay system was designed to measure the amount of the protein in normal human plasma, urine, and cerebrospinal fluid where the mean concentrations were found to be about 100, 10 and 0.3 mg/l, respectively. The protein was found to occur in normal plasma and urine as free monomers and dimers and as complexes with IgA and albumin.", "contents": "A complex-forming glycoprotein heterogeneous in charge and present in human plasma, urine, and cerebrospinal fluid. A glycoprotein from the urine of one healthy individual was purified by ultrafiltration, ion-exchange chromatography, gel chromatography and immunosorption. The protein contained only one polypeptide chain with an approximate molecular weight of 31 000 and was associated with a brown colour which did not disappear even after total reduction and alkylation of the protein followed by dialysis in 6 M guanidine hydrochloride. The protein appeared homogeneous on sodium dodecyl sulphate-polyacrylamide electrophoresis and gel chromatography. It had only one N-terminal amino acid sequence, Gly-Pro, and gave only one precipitate with a polyvalent antiserum but was found to be very heterorgeneous on agarose gel electrophoresis and on isoelectric focusing. Desialylation of the protein failed to alter this heterogeneity. An electroimmunoassay system was designed to measure the amount of the protein in normal human plasma, urine, and cerebrospinal fluid where the mean concentrations were found to be about 100, 10 and 0.3 mg/l, respectively. The protein was found to occur in normal plasma and urine as free monomers and dimers and as complexes with IgA and albumin.", "PMID": 952962}
{"id": "pubmed23n0032_352", "title": "An assessment of protein secondary structure prediction methods based on amino acid sequence.", "content": "Five of the several secondary structure prediction methods based on protein amino acid sequence has been computerized, allowing the calculation of joint prediction histograms which have been shown to be superior to any individual prediction. The known structures of about 40 proteins experimentally determined by X-ray crystallography are compared with the predictions resulting from calculated histograms. The accuracy of the predictions for helices is generally much better than for both beta-sheet regions and for turns. The overall agreement between prediction and observation within the amino terminal half of the protein molecules is clearly superior to that for the carboxyl half, suggesting an amino nucleating core. Predictions for smaller proteins and thermally stable proteins are generally good, indicating the sensitivity of the methods to short-range but not long-range interactions. In less than half the cases tested were the predictions useful; there was no way of knowing ahead of time if a favorable prediction would result. Given the lack of dramatic improvement with an increase in data base for the schemes and the generally poor agreement factors, it appears that a perfect predictive algorithm must include a consideration of energy minimization, thermalization, and long-range interactions. Extreme caution is suggested in applying present prediction routines to unknown protein structures.", "contents": "An assessment of protein secondary structure prediction methods based on amino acid sequence. Five of the several secondary structure prediction methods based on protein amino acid sequence has been computerized, allowing the calculation of joint prediction histograms which have been shown to be superior to any individual prediction. The known structures of about 40 proteins experimentally determined by X-ray crystallography are compared with the predictions resulting from calculated histograms. The accuracy of the predictions for helices is generally much better than for both beta-sheet regions and for turns. The overall agreement between prediction and observation within the amino terminal half of the protein molecules is clearly superior to that for the carboxyl half, suggesting an amino nucleating core. Predictions for smaller proteins and thermally stable proteins are generally good, indicating the sensitivity of the methods to short-range but not long-range interactions. In less than half the cases tested were the predictions useful; there was no way of knowing ahead of time if a favorable prediction would result. Given the lack of dramatic improvement with an increase in data base for the schemes and the generally poor agreement factors, it appears that a perfect predictive algorithm must include a consideration of energy minimization, thermalization, and long-range interactions. Extreme caution is suggested in applying present prediction routines to unknown protein structures.", "PMID": 952963}
{"id": "pubmed23n0032_353", "title": "Formation of dityrosine cross-links in proteins by oxidation of tyrosine residues.", "content": "1. Enzymic oxidation of proteins with peroxidase and hydrogenperoxide at a basic pH value leads to an oxidative phenolic coupling of adjacent tyrosine residues forming cross-linked proteins. 2. Dityrosine (3,3'-bityrosine) was identified as the cross-link in oxidised proteins by thin-layer chromatography, amino acid analysis and fluorescence measurements. 3. Gel filtration experiments with oxidised insulin showed that the cross-linkage is predominantly intermolecular. 4. In tetranitromethane treated proteins, dityrosine could be identified after hydrolysis.", "contents": "Formation of dityrosine cross-links in proteins by oxidation of tyrosine residues. 1. Enzymic oxidation of proteins with peroxidase and hydrogenperoxide at a basic pH value leads to an oxidative phenolic coupling of adjacent tyrosine residues forming cross-linked proteins. 2. Dityrosine (3,3'-bityrosine) was identified as the cross-link in oxidised proteins by thin-layer chromatography, amino acid analysis and fluorescence measurements. 3. Gel filtration experiments with oxidised insulin showed that the cross-linkage is predominantly intermolecular. 4. In tetranitromethane treated proteins, dityrosine could be identified after hydrolysis.", "PMID": 952964}
{"id": "pubmed23n0032_354", "title": "Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus).", "content": "A neurotoxin of the venom of the spider Latrodectus mactans tredecimguttatus, has been prepared in a homogeneous form and examined by a variety of techniques. The protein has a molecular weight of 130 000 and its toxicity in mice is about 49 000 LD50 mg pure protein/g body weight. The toxin releases norepinephrine from synaptosomes prepared from rat brain and shows most of the toxic effects of the crude venom preparation.", "contents": "Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus). A neurotoxin of the venom of the spider Latrodectus mactans tredecimguttatus, has been prepared in a homogeneous form and examined by a variety of techniques. The protein has a molecular weight of 130 000 and its toxicity in mice is about 49 000 LD50 mg pure protein/g body weight. The toxin releases norepinephrine from synaptosomes prepared from rat brain and shows most of the toxic effects of the crude venom preparation.", "PMID": 952966}
{"id": "pubmed23n0032_355", "title": "Reinterpretation of luminiscence properties of neurotoxins from the venom of Middle-Asian corba Naja oxiana eichw.", "content": "A new interpretation of previous work (Bukolova-Orlova, T. G., Burstein, E.A. and Yukelson, L. Ya (1974) Biochim. Biophys. Acta 342, 272-280) and some new data on the luminescence of neurotoxins I and II from Naja oxiana venom is given, based on the newer data on their complete amino acid sequences. Very effective excitation energy exchange exists between Trp-27 and Trp-33 in neurotoxin I and between Trp-27 and Trp-28 in neurotoxin II, Which results in the tryptophanyl fluorescence spectra of each of the proteins seeming to be monocomponent ones. The lowered fluorescence quantium yield value, the shortened phosphorescence lifetime (80% of the emission has tau p less than 0.5 s, 20% has tau p = 4.8 s, comparing with usual tau p = 5.5-5.9 s) and decreased phosphorescence to fluorescence ratio (0.042, as compared to the usual 0.4-0.7) for neurotoxin I suggest that the indole chromophore of Trp-27 and/or Trp-33 are in contact with heavy sulfur atoms of disulfide, most probably of Cys(28)-Cys(32). Tryptophanyls in neurotoxin II are exposed to the solvent, however their accessibility in relation to that of the free tryptophan to the negatively charged quencher I- (0.455) is much higher than that for the positively charged Cs+ (0.08), which is probably due to the proximity of cationic Lys-25, Lys-26 and His-31. The difference of accessibility to the negative and positive quenchers is even more pronounced in the case of the neurotoxin I (1.04 and 0 +/- 0.02, respectively), though in its chromophore vicinity along the primary structure there is only one cationic group, Lys-25. This fact together with the analysis of the amino acid sequence, suggest that the space folding of this polypeptide results in the close proximity of Trp-27 and/or Trp-33 with the C-terminal peptide segment 67-73, which contains four cationic groups (His-67, Lys-69, Lys-71 and Arg-72).", "contents": "Reinterpretation of luminiscence properties of neurotoxins from the venom of Middle-Asian corba Naja oxiana eichw. A new interpretation of previous work (Bukolova-Orlova, T. G., Burstein, E.A. and Yukelson, L. Ya (1974) Biochim. Biophys. Acta 342, 272-280) and some new data on the luminescence of neurotoxins I and II from Naja oxiana venom is given, based on the newer data on their complete amino acid sequences. Very effective excitation energy exchange exists between Trp-27 and Trp-33 in neurotoxin I and between Trp-27 and Trp-28 in neurotoxin II, Which results in the tryptophanyl fluorescence spectra of each of the proteins seeming to be monocomponent ones. The lowered fluorescence quantium yield value, the shortened phosphorescence lifetime (80% of the emission has tau p less than 0.5 s, 20% has tau p = 4.8 s, comparing with usual tau p = 5.5-5.9 s) and decreased phosphorescence to fluorescence ratio (0.042, as compared to the usual 0.4-0.7) for neurotoxin I suggest that the indole chromophore of Trp-27 and/or Trp-33 are in contact with heavy sulfur atoms of disulfide, most probably of Cys(28)-Cys(32). Tryptophanyls in neurotoxin II are exposed to the solvent, however their accessibility in relation to that of the free tryptophan to the negatively charged quencher I- (0.455) is much higher than that for the positively charged Cs+ (0.08), which is probably due to the proximity of cationic Lys-25, Lys-26 and His-31. The difference of accessibility to the negative and positive quenchers is even more pronounced in the case of the neurotoxin I (1.04 and 0 +/- 0.02, respectively), though in its chromophore vicinity along the primary structure there is only one cationic group, Lys-25. This fact together with the analysis of the amino acid sequence, suggest that the space folding of this polypeptide results in the close proximity of Trp-27 and/or Trp-33 with the C-terminal peptide segment 67-73, which contains four cationic groups (His-67, Lys-69, Lys-71 and Arg-72).", "PMID": 952967}
{"id": "pubmed23n0032_356", "title": "[Photosynthetic activity in the absence of CPl and CP2 pigmentary complexes (author's transl)].", "content": "Various photochemical activities were tested on chloroplasts of Zea mays that received 4 s of light every 4 h during the culture period. Photosystem I and Photosystem II were functioning, as well as the photosynthetic electron transport. These chloroplasts exhibited upon sodium dodecyl sulphate gel electrophoresis neither Complex 1 (Mr 70 000) generally associated with Photosystem I nor Complex 2 Mr 25 000) generally associated with Photosystem II. Chlorophyll is indeed attached to polypeptides of molecular weight 21 000 and 29 000. These results lead us to question the functional role of chloroplast protein-pigment complexes observed by sodium dodecyl sulphate gel electrophoresis.", "contents": "[Photosynthetic activity in the absence of CPl and CP2 pigmentary complexes (author's transl)]. Various photochemical activities were tested on chloroplasts of Zea mays that received 4 s of light every 4 h during the culture period. Photosystem I and Photosystem II were functioning, as well as the photosynthetic electron transport. These chloroplasts exhibited upon sodium dodecyl sulphate gel electrophoresis neither Complex 1 (Mr 70 000) generally associated with Photosystem I nor Complex 2 Mr 25 000) generally associated with Photosystem II. Chlorophyll is indeed attached to polypeptides of molecular weight 21 000 and 29 000. These results lead us to question the functional role of chloroplast protein-pigment complexes observed by sodium dodecyl sulphate gel electrophoresis.", "PMID": 952969}
{"id": "pubmed23n0032_357", "title": "Studies on the nature of the inhibitory effect of trypsin on the photosynthetic electron transport of system II in spinach chloroplasts.", "content": "The effect of trypsin on the photosynthetic electron transport of spinach chloroplasts has been investigated by measurements of the flash-induced absorption changes, indicating chlorophyll a1 at 703 nm, chlorophyll aII at 690 nm and at 515 nm via electrochromism the electrical potential gradient across the thylakoid membrane, respectively, and of the fluorescence induction caused by moderate actinic light. It was found: (1) In the presence of benzyl viologen as electron acceptor and with water as natural electron donor trypsin, incubation leads to a complete suppression of the absorption changes of the electrochromic effect and of chlorophyll aI and chlorophyll aII. (2) Addition of System I electron donors (N-methylphenazonium sulfate plus ascorbate or 2,6-dichlorophenolindphenol plus ascorbate) fully restores the chlorophyll aI photoreaction, whereas the initial amplitude of the electrochromic absorption change at 515 nm amounts about 50% of the control value without trypsin. The chlorophyll aII inhibition remains uneffected by System I electron donors. (3) System II electron donors (benzohydroquinone plus ascorbate or TPB) are unable to overcome the inhibition of electron transport by trypsin. (4) The fluorescence induction curve in 3-(3,4-dichlorophenyl)-1,1-dimethyl-urea-blocked chloroplasts is modified by trypsin. The level of maximal fluorescence is remarkably decreased, whereas the initial fluorescence remains constant. The rise in kinetics is slightly decelerated. From these results, it is concluded that in the linear electron transport from water to benzyl viologen, mild trypsin treatment specifically attacks System II at a site very close to the reaction center, either on the oxidizing or on the reducing side. The reaction center of System II itself is relatively stable against trypsin. Arguments are presented which argue in favor of the trypsin attack being primarily directed at the reducing side of System II.", "contents": "Studies on the nature of the inhibitory effect of trypsin on the photosynthetic electron transport of system II in spinach chloroplasts. The effect of trypsin on the photosynthetic electron transport of spinach chloroplasts has been investigated by measurements of the flash-induced absorption changes, indicating chlorophyll a1 at 703 nm, chlorophyll aII at 690 nm and at 515 nm via electrochromism the electrical potential gradient across the thylakoid membrane, respectively, and of the fluorescence induction caused by moderate actinic light. It was found: (1) In the presence of benzyl viologen as electron acceptor and with water as natural electron donor trypsin, incubation leads to a complete suppression of the absorption changes of the electrochromic effect and of chlorophyll aI and chlorophyll aII. (2) Addition of System I electron donors (N-methylphenazonium sulfate plus ascorbate or 2,6-dichlorophenolindphenol plus ascorbate) fully restores the chlorophyll aI photoreaction, whereas the initial amplitude of the electrochromic absorption change at 515 nm amounts about 50% of the control value without trypsin. The chlorophyll aII inhibition remains uneffected by System I electron donors. (3) System II electron donors (benzohydroquinone plus ascorbate or TPB) are unable to overcome the inhibition of electron transport by trypsin. (4) The fluorescence induction curve in 3-(3,4-dichlorophenyl)-1,1-dimethyl-urea-blocked chloroplasts is modified by trypsin. The level of maximal fluorescence is remarkably decreased, whereas the initial fluorescence remains constant. The rise in kinetics is slightly decelerated. From these results, it is concluded that in the linear electron transport from water to benzyl viologen, mild trypsin treatment specifically attacks System II at a site very close to the reaction center, either on the oxidizing or on the reducing side. The reaction center of System II itself is relatively stable against trypsin. Arguments are presented which argue in favor of the trypsin attack being primarily directed at the reducing side of System II.", "PMID": 952970}
{"id": "pubmed23n0032_358", "title": "Studies on the structural and functional organization of system II of photosynthesis. The use of trypsin as a structurally selective inhibitor at the outer surface of the thylakoid membrane.", "content": "The effect of trypsin on the photosynthetic electron transport has been investigated in the presence of various electron acceptors (benzyl viologen, p-benzo-quinone, K3[Fe(CN)6]) by measurements of flash-induced oxygen evolution and of the absorption changes at 334 nm, indicating the primary electron acceptor of System II, X 320, and at 515 nm, indicating via electrochromism the electrical potential gradient across the thylakoid membrane. It was found that the effect of trypsin is strongly dependent on the nature of the electron acceptor: (1) Oxygen evolution is completely inhibited in the presence of p-benzo-quinone, but remains nearly unaffected by K3[Fe(CN)]6. (2) The initial amplitude deltaAO of the 334 nm absorption change is insensitive to trypsin in the presence of K3[Fe(CN)6], but the absorption change is abolished if benzyl viologen is used as acceptor. (3) The initial amplitude deltaAO of the 515 nm absorption change decreases by trypsin down to 50% with K3[Fe(CN)6] and is completely suppressed with benzyl viologen. (4) In trypsinated chloroplasts, the above-mentioned activities appear to be rather insensitive to 3-(3,4-dichlorophenyl)-1,1-dimethylurea, in contrast to normal chloroplasts. On the basis of these results it is inferred that the primary electron acceptor of System II, X 320, is covered by a proteinaceous component susceptible to tryptic digestion. In addition, it is postulated that this component acts as well as an allosteric protein responsible for the regulation of the electronic interaction between X 320 and the plastoquinone pool, as for the inhibitory effect of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Various other possible effects caused by the proteinaceous shield and its modification by trypsin are discussed. The present results are in complete agreement with asymmetric membrane models postulating a zig-zag arrangement of the electron transport chain with the reducing side located towards the outer phase and the oxidizing side near the inner phase of the thylakoids.", "contents": "Studies on the structural and functional organization of system II of photosynthesis. The use of trypsin as a structurally selective inhibitor at the outer surface of the thylakoid membrane. The effect of trypsin on the photosynthetic electron transport has been investigated in the presence of various electron acceptors (benzyl viologen, p-benzo-quinone, K3[Fe(CN)6]) by measurements of flash-induced oxygen evolution and of the absorption changes at 334 nm, indicating the primary electron acceptor of System II, X 320, and at 515 nm, indicating via electrochromism the electrical potential gradient across the thylakoid membrane. It was found that the effect of trypsin is strongly dependent on the nature of the electron acceptor: (1) Oxygen evolution is completely inhibited in the presence of p-benzo-quinone, but remains nearly unaffected by K3[Fe(CN)]6. (2) The initial amplitude deltaAO of the 334 nm absorption change is insensitive to trypsin in the presence of K3[Fe(CN)6], but the absorption change is abolished if benzyl viologen is used as acceptor. (3) The initial amplitude deltaAO of the 515 nm absorption change decreases by trypsin down to 50% with K3[Fe(CN)6] and is completely suppressed with benzyl viologen. (4) In trypsinated chloroplasts, the above-mentioned activities appear to be rather insensitive to 3-(3,4-dichlorophenyl)-1,1-dimethylurea, in contrast to normal chloroplasts. On the basis of these results it is inferred that the primary electron acceptor of System II, X 320, is covered by a proteinaceous component susceptible to tryptic digestion. In addition, it is postulated that this component acts as well as an allosteric protein responsible for the regulation of the electronic interaction between X 320 and the plastoquinone pool, as for the inhibitory effect of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Various other possible effects caused by the proteinaceous shield and its modification by trypsin are discussed. The present results are in complete agreement with asymmetric membrane models postulating a zig-zag arrangement of the electron transport chain with the reducing side located towards the outer phase and the oxidizing side near the inner phase of the thylakoids.", "PMID": 952971}
{"id": "pubmed23n0032_359", "title": "The effect of magnesium ions on action spectra for reactions mediated  by photosystems I and II in spinach chloroplasts.", "content": "Action spectra were measured for positive changes in variable fluorescence (emission greater than 665 nm) excited by a beam of 485 nm chopped at 75 HZ. The action of two further beams were compared, one being variable, the other (reference) constant with respect to wavelength and intensity. Comparison was achieved by alternating the reference and the variable wavelength beams at 0.3 HZ and adjusting the intensity of the latter such as to cancel out any 0.3 HZ component in the 75 HZ fluorescence signal. The relative action then was obtained as the reciprocal of the intensity of the variable wavelength beam. Similarly, action spectra were measured for O2 evolution with ferricyanide/p-phenylenediamine as electron acceptor, and for O2 uptake mediated by methyl viologen with ascorbate 3-(p-chlorophenyl)-1,1-dimethylurea as electron donor in the presence of 2,6-dichlorophenolindophenol. Addition of 5 mM MgCl2 increases the relative action around 480 nm for the change in variable fluorescence and p-phenylenediamine-dependent O2 evolution, and decreases it for methyl viologen-mediated O2 uptake with 2,6-dichlorophenolindo-phenol/ascorbate as electron donor in the presence of 3-(p-chlorophenyl)-1,1-dimethylurea. The change in variable fluorescence and O2 evolution are stimulated by MgCl2, whereas O2 uptake is inhibited by it. The results are discussed in terms of a model assuming a tripartite organization of the photosynthetic pigments (Thornber, J. P. and Highkin, H. R. (1974) Eur. J. Biochem. 41, 109-116; Butler, W. L. and Kitajima, M. (1975) Biochim. Biophys. Acta 396, 72-85). MgCl2 is thought to promote energy transfer to Photosystem II from a light-harvesting pigment complex serving both photosystems.", "contents": "The effect of magnesium ions on action spectra for reactions mediated  by photosystems I and II in spinach chloroplasts. Action spectra were measured for positive changes in variable fluorescence (emission greater than 665 nm) excited by a beam of 485 nm chopped at 75 HZ. The action of two further beams were compared, one being variable, the other (reference) constant with respect to wavelength and intensity. Comparison was achieved by alternating the reference and the variable wavelength beams at 0.3 HZ and adjusting the intensity of the latter such as to cancel out any 0.3 HZ component in the 75 HZ fluorescence signal. The relative action then was obtained as the reciprocal of the intensity of the variable wavelength beam. Similarly, action spectra were measured for O2 evolution with ferricyanide/p-phenylenediamine as electron acceptor, and for O2 uptake mediated by methyl viologen with ascorbate 3-(p-chlorophenyl)-1,1-dimethylurea as electron donor in the presence of 2,6-dichlorophenolindophenol. Addition of 5 mM MgCl2 increases the relative action around 480 nm for the change in variable fluorescence and p-phenylenediamine-dependent O2 evolution, and decreases it for methyl viologen-mediated O2 uptake with 2,6-dichlorophenolindo-phenol/ascorbate as electron donor in the presence of 3-(p-chlorophenyl)-1,1-dimethylurea. The change in variable fluorescence and O2 evolution are stimulated by MgCl2, whereas O2 uptake is inhibited by it. The results are discussed in terms of a model assuming a tripartite organization of the photosynthetic pigments (Thornber, J. P. and Highkin, H. R. (1974) Eur. J. Biochem. 41, 109-116; Butler, W. L. and Kitajima, M. (1975) Biochim. Biophys. Acta 396, 72-85). MgCl2 is thought to promote energy transfer to Photosystem II from a light-harvesting pigment complex serving both photosystems.", "PMID": 952972}
{"id": "pubmed23n0032_360", "title": "A demonstration of energy transfer from photosystem II to photosystem I in chloroplasts.", "content": "Photosystem I activity of Tris-washed chloroplasts was measured at room temperature as the rate of photoreduction of NADP and as the rate of oxygen uptake mediated  by methyl viologen in both cases using dichlorophenolindophenol plus ascorbate as the source of electrons for Photosystem I. With both assay systems the rate of electron transport by Photosystem  I was stimulated approx. 20% by the addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea which caused the Photosystem II reaction centers to close. Photosystem I activity of chloroplasts was measured at low temperature as the rate of photooxidation of P-700. Chloroplasts suspended in the presence of hydroxylamine and 3-(3,4-dichlorophenyl)-1,1-dimethylurea were frozen to -196 degrees C after adaptation to darkness or after a preillumination at room temperature. The Photosystem II reaction centers of the frozen dark-adapted sample were all open; those of the preilluminated sample were all closed. The rate of photooxidation of P-700 at -196 degrees C with the preilluminated sample was approx. 25% faster than with the dark-adapted sample. We conclude from both the room temperature and the low temperature experiments that there is greater energy transfer from Photosystem II to Photosystem I when the Photosystem II reaction centers are closed and that these results are a direct demonstration of spillover.", "contents": "A demonstration of energy transfer from photosystem II to photosystem I in chloroplasts. Photosystem I activity of Tris-washed chloroplasts was measured at room temperature as the rate of photoreduction of NADP and as the rate of oxygen uptake mediated  by methyl viologen in both cases using dichlorophenolindophenol plus ascorbate as the source of electrons for Photosystem I. With both assay systems the rate of electron transport by Photosystem  I was stimulated approx. 20% by the addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea which caused the Photosystem II reaction centers to close. Photosystem I activity of chloroplasts was measured at low temperature as the rate of photooxidation of P-700. Chloroplasts suspended in the presence of hydroxylamine and 3-(3,4-dichlorophenyl)-1,1-dimethylurea were frozen to -196 degrees C after adaptation to darkness or after a preillumination at room temperature. The Photosystem II reaction centers of the frozen dark-adapted sample were all open; those of the preilluminated sample were all closed. The rate of photooxidation of P-700 at -196 degrees C with the preilluminated sample was approx. 25% faster than with the dark-adapted sample. We conclude from both the room temperature and the low temperature experiments that there is greater energy transfer from Photosystem II to Photosystem I when the Photosystem II reaction centers are closed and that these results are a direct demonstration of spillover.", "PMID": 952973}
{"id": "pubmed23n0032_361", "title": "Picosecond kinetics of chlorophyll and chlorophyll/quinone solutions in ethanol.", "content": "The mechanism of quenching by quinones of the lowest excited singlet state of chlorophyll has been investigated using picosecond laser spectroscopy. With chlorophyll alone, laser excitation resulted in immediate (less than 10 ps) bleaching of the 665 nm band and production of new absorption bands in the regions 460-550 and 800-830 nm. The lifetimes of these changes were greater than 500 ps. Addition of 2,6-dimethylbenzoquinone caused quenching of these absorbance changes. No indication of chlorophyll cation radical formation was obtained. Thus, the interaction between quinone and the chlorophyll excited singlet state results in energy dissipation without measurable formation of radical species having lifetimes longer than 10 ps. This is in marked contrast to the quenching of the chlorophyll lowest triplet state by quinones, during which easily detectable stable radical formation has been observed.", "contents": "Picosecond kinetics of chlorophyll and chlorophyll/quinone solutions in ethanol. The mechanism of quenching by quinones of the lowest excited singlet state of chlorophyll has been investigated using picosecond laser spectroscopy. With chlorophyll alone, laser excitation resulted in immediate (less than 10 ps) bleaching of the 665 nm band and production of new absorption bands in the regions 460-550 and 800-830 nm. The lifetimes of these changes were greater than 500 ps. Addition of 2,6-dimethylbenzoquinone caused quenching of these absorbance changes. No indication of chlorophyll cation radical formation was obtained. Thus, the interaction between quinone and the chlorophyll excited singlet state results in energy dissipation without measurable formation of radical species having lifetimes longer than 10 ps. This is in marked contrast to the quenching of the chlorophyll lowest triplet state by quinones, during which easily detectable stable radical formation has been observed.", "PMID": 952974}
{"id": "pubmed23n0032_362", "title": "Control of oxidative phosphorylation by the extra-mitochondrial ATP/ADP ratio.", "content": "The control of mitochondrial ATP synthesis by the extramitochondrial adenine nucleotide pattern was investigated with rat liver mitochondria. It is demonstrated that any stationary state between the two limit states of maximum activity (state 3) and of resting activity (state 4) can be obtained by a hexokinase-glucose trap as an ADP-regenerating system. These intermediate states are characterized by stationary respiratory rates, stationary redox levels of the cytochromes b and c and stationary levels of extramitochondrial ATP and ADP between the rates and levels of the limit states. At a constant concentration of inorganic phosphate the activity of mitochondria between the limit states is controlled by the extramitochondrial ATP/ADP ratio independent of the total concentration of adenine nucleotides present. The control range was found to be between ratios of about 5 and 100 at 10 mM phosphate. At lower ratios the mitochondria are in their maximum phosphorylating state. With succinate+rotenone and glutamate+malate the same control range was observed, indicating that it is independent of the nature of substrate oxidized. The results suggest that in the control range the mitochondrial activity is limited by the competition of ADP and ATP for the adenine nucleotide translocator.", "contents": "Control of oxidative phosphorylation by the extra-mitochondrial ATP/ADP ratio. The control of mitochondrial ATP synthesis by the extramitochondrial adenine nucleotide pattern was investigated with rat liver mitochondria. It is demonstrated that any stationary state between the two limit states of maximum activity (state 3) and of resting activity (state 4) can be obtained by a hexokinase-glucose trap as an ADP-regenerating system. These intermediate states are characterized by stationary respiratory rates, stationary redox levels of the cytochromes b and c and stationary levels of extramitochondrial ATP and ADP between the rates and levels of the limit states. At a constant concentration of inorganic phosphate the activity of mitochondria between the limit states is controlled by the extramitochondrial ATP/ADP ratio independent of the total concentration of adenine nucleotides present. The control range was found to be between ratios of about 5 and 100 at 10 mM phosphate. At lower ratios the mitochondria are in their maximum phosphorylating state. With succinate+rotenone and glutamate+malate the same control range was observed, indicating that it is independent of the nature of substrate oxidized. The results suggest that in the control range the mitochondrial activity is limited by the competition of ADP and ATP for the adenine nucleotide translocator.", "PMID": 952975}
{"id": "pubmed23n0032_363", "title": "Cooperative binding of calcium to glycerinated skeletal muscle fibers.", "content": "The binding of 45Ca2+ to glycerinated rabbit psoas fibers was measured by means of a double isotope technique. With 5 mM Mg2+ (no ATP) binding was half-maximal at 1.4-10(-6) M Ca2+ and the maximal amount bound was 1.6 mumol/g protein. At less than 50% saturation, the Scatchard plot had a positive slope and the Hill coefficient was 2.2. At greater than 50% saturation, the Scatchard plot was linear with a negative slope (K' = 0.8 - (10(6) M-1) and the Hill coefficient was 1.0. In the absence of Mg2+, binding was half-maximal at 3 - 10(-7) M Ca2+ and the maximal amount bound was 2.9 mumol/g protein. The Scatchard plot indicated two classes of sites with K' values of about 2 - 10(7) and 2 - 10(6) M-1. The Hill coefficient in the mid-saturation range was approx. 0.6. The data indicate that in the presence of the Mg2+ binding to about half of the total Ca2+ binding sites is suppressed and there is a strong positive cooperativity involving half of the ramaining sites.", "contents": "Cooperative binding of calcium to glycerinated skeletal muscle fibers. The binding of 45Ca2+ to glycerinated rabbit psoas fibers was measured by means of a double isotope technique. With 5 mM Mg2+ (no ATP) binding was half-maximal at 1.4-10(-6) M Ca2+ and the maximal amount bound was 1.6 mumol/g protein. At less than 50% saturation, the Scatchard plot had a positive slope and the Hill coefficient was 2.2. At greater than 50% saturation, the Scatchard plot was linear with a negative slope (K' = 0.8 - (10(6) M-1) and the Hill coefficient was 1.0. In the absence of Mg2+, binding was half-maximal at 3 - 10(-7) M Ca2+ and the maximal amount bound was 2.9 mumol/g protein. The Scatchard plot indicated two classes of sites with K' values of about 2 - 10(7) and 2 - 10(6) M-1. The Hill coefficient in the mid-saturation range was approx. 0.6. The data indicate that in the presence of the Mg2+ binding to about half of the total Ca2+ binding sites is suppressed and there is a strong positive cooperativity involving half of the ramaining sites.", "PMID": 952976}
{"id": "pubmed23n0032_364", "title": "Positional and fatty acid specificity of monoacyl- and diacylglycerol 3-phosphate formation by rabbit heart microsomes.", "content": "Fatty acid selectivity of acyl-CoA:glycerol-3-phosphate acyltransferase (EC 2.3.1.15) and acyl-CoA:monoacylglycerol-3-phosphate acyltransferase (EC 2.3.1.52) of the microsomal fraction prepared from rabbit heart was studied. 1. The rate of acylation of glycerol 3-phosphate was increased proportionally with the concentration of acyl-CoA. The maximum rate was reached at 0.3 mumol acyl-CoA per ml. Palmitoyl-, oleoyl- and linoleoyl-CoA all served equally well as acyl donors, and produced approximately equal amounts of mono- and diacylglycerol 3-phosphate. The rate of reaction measured in the presence of two acyl-CoA esters was similar to that in the presence of a single acyl-CoA. 2. Treatment of synthesized monoacylglycerol 3-phosphate with Crotalus adamanteus venom phospholipase A2 (EC 3.1.1.4) and that with phosphatidate phosphatase (EC 3.1.3.4) indicated that the heart enzyme synthesized almost exclusively 1-acylglycerol 3-phosphate regardless of the kind of acyl donor. 3. Hydrolysis of diacylglycerol 3-phosphate with phospholipase A2 revealed that there was a slight preference for linoleoyl-CoA at position 2 and a slight discrimination against palmitoyl- and stearoyl-CoA at position 2, when diacylglycerol 3-phosphate was synthesized in the presence of a mixture of acyl-CoA esters. When diacylglycerol 3-phosphate was formed in the presence of a single acyl-CoA, the fatty acid distribution was random. 4. The rate of acylation of 1-palmitoylglycerol 3-phosphate by rabbit heart microsomal fraction was increased proportionally to the increasing concentrations of 1-palmitoylglycerol 3-phosphate up to 50 nmol per ml; higher concentrations were inhibitory. Differences in the activities measured with palmitoyl-, oleoyl- and linoleoyl-CoA as acyl donors were negligible. When stearoyl-, arachidonoyl- and erucoyl-CoA acted as acyl donors, the rates of reaction were low. 5. The acyl-CoA:1-palmitoylglycerol-3-phosphate acyltransferase activity increased proportionally to the increasing concentrations of acyl-CoA up to 10 nmol per ml; acyl donor specificity was similar to that found above [4]. The acyltransferase showed", "contents": "Positional and fatty acid specificity of monoacyl- and diacylglycerol 3-phosphate formation by rabbit heart microsomes. Fatty acid selectivity of acyl-CoA:glycerol-3-phosphate acyltransferase (EC 2.3.1.15) and acyl-CoA:monoacylglycerol-3-phosphate acyltransferase (EC 2.3.1.52) of the microsomal fraction prepared from rabbit heart was studied. 1. The rate of acylation of glycerol 3-phosphate was increased proportionally with the concentration of acyl-CoA. The maximum rate was reached at 0.3 mumol acyl-CoA per ml. Palmitoyl-, oleoyl- and linoleoyl-CoA all served equally well as acyl donors, and produced approximately equal amounts of mono- and diacylglycerol 3-phosphate. The rate of reaction measured in the presence of two acyl-CoA esters was similar to that in the presence of a single acyl-CoA. 2. Treatment of synthesized monoacylglycerol 3-phosphate with Crotalus adamanteus venom phospholipase A2 (EC 3.1.1.4) and that with phosphatidate phosphatase (EC 3.1.3.4) indicated that the heart enzyme synthesized almost exclusively 1-acylglycerol 3-phosphate regardless of the kind of acyl donor. 3. Hydrolysis of diacylglycerol 3-phosphate with phospholipase A2 revealed that there was a slight preference for linoleoyl-CoA at position 2 and a slight discrimination against palmitoyl- and stearoyl-CoA at position 2, when diacylglycerol 3-phosphate was synthesized in the presence of a mixture of acyl-CoA esters. When diacylglycerol 3-phosphate was formed in the presence of a single acyl-CoA, the fatty acid distribution was random. 4. The rate of acylation of 1-palmitoylglycerol 3-phosphate by rabbit heart microsomal fraction was increased proportionally to the increasing concentrations of 1-palmitoylglycerol 3-phosphate up to 50 nmol per ml; higher concentrations were inhibitory. Differences in the activities measured with palmitoyl-, oleoyl- and linoleoyl-CoA as acyl donors were negligible. When stearoyl-, arachidonoyl- and erucoyl-CoA acted as acyl donors, the rates of reaction were low. 5. The acyl-CoA:1-palmitoylglycerol-3-phosphate acyltransferase activity increased proportionally to the increasing concentrations of acyl-CoA up to 10 nmol per ml; acyl donor specificity was similar to that found above [4]. The acyltransferase showed", "PMID": 952977}
{"id": "pubmed23n0032_365", "title": "The lipid intermediates arising during glycoprotein biosynthesis in liver microsomes.", "content": "Incubation of liver microsomes with GDP [14C] mannose leads to the formation of lipid-linked derivatives of [14C] mannose, a dolichol phosphate monosaccharide and dolichol pyrophosphate oligosaccharides. Standard procedures for separating these two types of compounds from each other were found to be deficient in that fractions thought to contain only dolichol pyrophosphate oligosaccharides are contaminated with dolichol phosphate mannose. This paper presents a column chromatographic procedure which conveniently separates the products of an 8 min labeling experiment into two components; dolichol phosphate [14C]mannose and a [14C]-mannose containing oligosaccharide which is also lipid bound. When this oligosaccharide is released from the lipid by hydrolysis and chromatographed on Sephadex G-50 or G-15 it gives a single peak with an indicated molecular weight of 1100. However, when this released oligosaccharide is chromatographed on concanavalin A Sepharose it is resolved into two peaks suggesting that there may be 2 oligosaccharide of approximately the same size but different structures. After brief periods of labeling with GDP [14C]mannose (5 s) an additional oligosaccharide of 3 to 4 sugar residues can be found in the dolichol pyrophosphate oligosaccharides fraction. Incubation of liver microsomes with UDP [14C]glucose or UDP[14C]galactose produces oligosaccharide components containing 7--8 sugar residues. Labeling of microsomes with UDP[14C]acetylglucosamine gives rise to three different components, including a lipid bound oligosaccharide containing 3- 5 sugar residues.", "contents": "The lipid intermediates arising during glycoprotein biosynthesis in liver microsomes. Incubation of liver microsomes with GDP [14C] mannose leads to the formation of lipid-linked derivatives of [14C] mannose, a dolichol phosphate monosaccharide and dolichol pyrophosphate oligosaccharides. Standard procedures for separating these two types of compounds from each other were found to be deficient in that fractions thought to contain only dolichol pyrophosphate oligosaccharides are contaminated with dolichol phosphate mannose. This paper presents a column chromatographic procedure which conveniently separates the products of an 8 min labeling experiment into two components; dolichol phosphate [14C]mannose and a [14C]-mannose containing oligosaccharide which is also lipid bound. When this oligosaccharide is released from the lipid by hydrolysis and chromatographed on Sephadex G-50 or G-15 it gives a single peak with an indicated molecular weight of 1100. However, when this released oligosaccharide is chromatographed on concanavalin A Sepharose it is resolved into two peaks suggesting that there may be 2 oligosaccharide of approximately the same size but different structures. After brief periods of labeling with GDP [14C]mannose (5 s) an additional oligosaccharide of 3 to 4 sugar residues can be found in the dolichol pyrophosphate oligosaccharides fraction. Incubation of liver microsomes with UDP [14C]glucose or UDP[14C]galactose produces oligosaccharide components containing 7--8 sugar residues. Labeling of microsomes with UDP[14C]acetylglucosamine gives rise to three different components, including a lipid bound oligosaccharide containing 3- 5 sugar residues.", "PMID": 952978}
{"id": "pubmed23n0032_366", "title": "Rat intestinal glycolipids. II. Distribution and biosynthesis of glycolipids and ceramide in villus and crypt cells.", "content": "Intestinal epithelial cells were isolated from rat intestine and grouped into villus and crypt cell fractions. Glycolipids were purified from each cell fraction and quantitated by fluorimetric determination of glycolipid sphingosine. Significant quantities of ceramide were found in all cell fractions and accounted for approximately 15% of total glycolipid sphingosine. While villus and crypt cell fractions quantitatively contained differing amounts of sphingosine, all cell fractions contained proportionally similar quantities of sphingosine when compared to cellular cholesterol or phospholipid. Individual glycolipids, however, showed significant differences in distribution between villus and crypt cells. Hematoside and glucosylceramide were proportionally increased in villus cells, while crypt cells showed an increase in trihexosylceramide and ceramide content. The rate of UDPglucose : hydroxy fatty acid ceramide glucosyltransferase was higher in villus cells while the rate of UDPgalactose : lactosylceramide galactosyltransferase was 3--4 times increased in crypt cells. These studies demonstrate that significant differences in both the distribution and biosynthesis of individual glycolipids occur in crypt and villus cells of rat intestine and are of possible importance in the process of intestinal cell differentiation.", "contents": "Rat intestinal glycolipids. II. Distribution and biosynthesis of glycolipids and ceramide in villus and crypt cells. Intestinal epithelial cells were isolated from rat intestine and grouped into villus and crypt cell fractions. Glycolipids were purified from each cell fraction and quantitated by fluorimetric determination of glycolipid sphingosine. Significant quantities of ceramide were found in all cell fractions and accounted for approximately 15% of total glycolipid sphingosine. While villus and crypt cell fractions quantitatively contained differing amounts of sphingosine, all cell fractions contained proportionally similar quantities of sphingosine when compared to cellular cholesterol or phospholipid. Individual glycolipids, however, showed significant differences in distribution between villus and crypt cells. Hematoside and glucosylceramide were proportionally increased in villus cells, while crypt cells showed an increase in trihexosylceramide and ceramide content. The rate of UDPglucose : hydroxy fatty acid ceramide glucosyltransferase was higher in villus cells while the rate of UDPgalactose : lactosylceramide galactosyltransferase was 3--4 times increased in crypt cells. These studies demonstrate that significant differences in both the distribution and biosynthesis of individual glycolipids occur in crypt and villus cells of rat intestine and are of possible importance in the process of intestinal cell differentiation.", "PMID": 952979}
{"id": "pubmed23n0032_367", "title": "A comparative study of the glycolipids of human, bird and fish testes and of human sperm.", "content": "The glycolipids of human testis and sperm have been compared. Both adult testis and the sperm exhibited remarkably complex, but generally similar, patterns of glycolipids. In particular, both contained appreciable amounts of the sulfogalactosylmonoalkylmonoacylglycerol, recently shown to be the principal glycolipid of the testis and sperm of a number of animals. In contrast, immature (prebuteral) human testis did not contain this compound. To extend knowledge on the possible distribution of sulfogalactosylmonoalkylmonoacylglycerol in the testes of other chordates, we have also analysed the glycolipids of the testes of a number of birds and fish. None of the testes from these species contained the above compound. Instead, sulfogalactosylceramide was found to be a major glycolipid of the testis of mature fowl, duck and skate-fish and sulfogalactosylglucosylceramide of the testis of mature salmon and trout. Immature duck testis contained only a trace of sulfogalactosylceramide. These studies reveal intriguing differences between the sulfatides of various chordates, lend support to the concept that sulfatides increase markedly in testis at a specific stage of spermatogenesis and suggest an important role for sulfatides in testicular and spermatozoal function.", "contents": "A comparative study of the glycolipids of human, bird and fish testes and of human sperm. The glycolipids of human testis and sperm have been compared. Both adult testis and the sperm exhibited remarkably complex, but generally similar, patterns of glycolipids. In particular, both contained appreciable amounts of the sulfogalactosylmonoalkylmonoacylglycerol, recently shown to be the principal glycolipid of the testis and sperm of a number of animals. In contrast, immature (prebuteral) human testis did not contain this compound. To extend knowledge on the possible distribution of sulfogalactosylmonoalkylmonoacylglycerol in the testes of other chordates, we have also analysed the glycolipids of the testes of a number of birds and fish. None of the testes from these species contained the above compound. Instead, sulfogalactosylceramide was found to be a major glycolipid of the testis of mature fowl, duck and skate-fish and sulfogalactosylglucosylceramide of the testis of mature salmon and trout. Immature duck testis contained only a trace of sulfogalactosylceramide. These studies reveal intriguing differences between the sulfatides of various chordates, lend support to the concept that sulfatides increase markedly in testis at a specific stage of spermatogenesis and suggest an important role for sulfatides in testicular and spermatozoal function.", "PMID": 952980}
{"id": "pubmed23n0032_368", "title": "In vitro incorporation of isomeric cis-octadecenoic acids by rat liver mitochondria.", "content": "The metabolic fate of positional isomers of cis-octadecenoic acids was compared to that of oleic acid, elaidic acid and stearic acid in rat liver mitochondria. The positional isomers as well as elaidic acid and stearic acid were labelled with 3H and they were incubated in pairs with [1-14C]oleic acid. 3H/14C ratios were determined for the administered mixtures and for the isolated lipid classes. The results suggested that all isomers were readily incorporated into the membraneous structure of mitochondria. Those with the double bond in the middle of the acyl chain resembled oleic acid and they were preferentially incorporated in cholesterol esters, triacylglycerols and in the 2-position of triacylglycerols and of phosphatidylcholines. Those with the double bond away from the middle of the chain were metabolically distinct from oleic acid and behaved like trans fatty acids. They were rapidly taken up by mitochondria. They were preferentially incorporated in phospholipids and they occupied the 1-position in phosphatidylcholines and the 1- and 3-positions in triacylglycerols.", "contents": "In vitro incorporation of isomeric cis-octadecenoic acids by rat liver mitochondria. The metabolic fate of positional isomers of cis-octadecenoic acids was compared to that of oleic acid, elaidic acid and stearic acid in rat liver mitochondria. The positional isomers as well as elaidic acid and stearic acid were labelled with 3H and they were incubated in pairs with [1-14C]oleic acid. 3H/14C ratios were determined for the administered mixtures and for the isolated lipid classes. The results suggested that all isomers were readily incorporated into the membraneous structure of mitochondria. Those with the double bond in the middle of the acyl chain resembled oleic acid and they were preferentially incorporated in cholesterol esters, triacylglycerols and in the 2-position of triacylglycerols and of phosphatidylcholines. Those with the double bond away from the middle of the chain were metabolically distinct from oleic acid and behaved like trans fatty acids. They were rapidly taken up by mitochondria. They were preferentially incorporated in phospholipids and they occupied the 1-position in phosphatidylcholines and the 1- and 3-positions in triacylglycerols.", "PMID": 952981}
{"id": "pubmed23n0032_369", "title": "Effects of the thyroid state on cholesterol metabolism in the rat.", "content": "An isotopic equilibrium method which permits the in vivo measurements of cholesterol turnover processes was applied to different groups of rats: (1) radiothyroidectomized, (2) low-iodine fed, and (3) L-thyroxin fed. Plasma cholesterol concentrations were enhanced after thyroidectomy and reduced by large dose of L-thyroxin. A low-iodine diet decreased plasma thyroxin level but did not affect plasma cholesterol. Thyroid levels in plasma modified the coefficient of intestinal absorption of cholesterol. After thyroidectomy or under conditions of reduced thyroxin formation this absorption coefficient was enhanced. The absorption coefficient of cholesterol was decreased in rats receiving 31 or 61.5 mug/day of L-thyroxin but was not changed in rats fed 110 mug/day L-thyroxin. The proportion of de novo biosynthetisized cholesterol eliminated into the feces (external secretion) was reduced while thyroxin levels were low. The fecal excretion of cholesterol and the in vitro exchange of cholesterol between erythrocytes and plasma were increased by L-thyroxin ingestion. The rate of cholesterol biosynthesis was decreased after thyroidectomy and enhanced by L-thyroxin feeding. In fact, changes in thyroid state modified indirectly the biosynthesis of cholesterol by its effects on metabolism and on the coefficient of intestinal absorption of cholesterol.", "contents": "Effects of the thyroid state on cholesterol metabolism in the rat. An isotopic equilibrium method which permits the in vivo measurements of cholesterol turnover processes was applied to different groups of rats: (1) radiothyroidectomized, (2) low-iodine fed, and (3) L-thyroxin fed. Plasma cholesterol concentrations were enhanced after thyroidectomy and reduced by large dose of L-thyroxin. A low-iodine diet decreased plasma thyroxin level but did not affect plasma cholesterol. Thyroid levels in plasma modified the coefficient of intestinal absorption of cholesterol. After thyroidectomy or under conditions of reduced thyroxin formation this absorption coefficient was enhanced. The absorption coefficient of cholesterol was decreased in rats receiving 31 or 61.5 mug/day of L-thyroxin but was not changed in rats fed 110 mug/day L-thyroxin. The proportion of de novo biosynthetisized cholesterol eliminated into the feces (external secretion) was reduced while thyroxin levels were low. The fecal excretion of cholesterol and the in vitro exchange of cholesterol between erythrocytes and plasma were increased by L-thyroxin ingestion. The rate of cholesterol biosynthesis was decreased after thyroidectomy and enhanced by L-thyroxin feeding. In fact, changes in thyroid state modified indirectly the biosynthesis of cholesterol by its effects on metabolism and on the coefficient of intestinal absorption of cholesterol.", "PMID": 952982}
{"id": "pubmed23n0032_370", "title": "Linoleic acid desaturation activity of liver microsomes of essential fatty acid deficient and sufficient rats.", "content": "Studies were carried out to relate the changes of the fatty acid and lipid composition of rat microsomes with the modification of the activity of the linoleic acid desaturation evoked by an essential fatty acid deficient diet. Two steps were shown in the progression of the essential fatty acid deficienty. In a first step shown at three days of essential fatty acid deficiency the fatty acid composition was changed by decreasing linoleic and arachidonic acids and increasing oleic and eicosatrienoic (-9) acids. No change was found in the lipid distribution and approximate V and Km of the linoleic acid desaturation. In this first step the unsaturated/saturated fatty acids ratio fell in spite of the synthesis of eicosatrienoic (n-9) acid that was produced without any change of enzyme activity. In a second step shown at 15 days of essential fatty acid deficiency the change of the fatty acid composition was greater but the unsaturated/saturated acid ratio was restored. An increase of triacylglycerols and a decrease of phospholipids was also detected together with an enhanced activity of linoleic acid desaturation (higher approximate V) and a higher approximate Km. The increase of the V of linoleic acid desaturation is considered to be evoked by an increased level of active delta-6 desaturase. The increased activity of the delta-6 desaturase in this second period is a secondary and important response of the cell to maintain the unsaturated : saturated acid ratio and fluidity of the membrane.", "contents": "Linoleic acid desaturation activity of liver microsomes of essential fatty acid deficient and sufficient rats. Studies were carried out to relate the changes of the fatty acid and lipid composition of rat microsomes with the modification of the activity of the linoleic acid desaturation evoked by an essential fatty acid deficient diet. Two steps were shown in the progression of the essential fatty acid deficienty. In a first step shown at three days of essential fatty acid deficiency the fatty acid composition was changed by decreasing linoleic and arachidonic acids and increasing oleic and eicosatrienoic (-9) acids. No change was found in the lipid distribution and approximate V and Km of the linoleic acid desaturation. In this first step the unsaturated/saturated fatty acids ratio fell in spite of the synthesis of eicosatrienoic (n-9) acid that was produced without any change of enzyme activity. In a second step shown at 15 days of essential fatty acid deficiency the change of the fatty acid composition was greater but the unsaturated/saturated acid ratio was restored. An increase of triacylglycerols and a decrease of phospholipids was also detected together with an enhanced activity of linoleic acid desaturation (higher approximate V) and a higher approximate Km. The increase of the V of linoleic acid desaturation is considered to be evoked by an increased level of active delta-6 desaturase. The increased activity of the delta-6 desaturase in this second period is a secondary and important response of the cell to maintain the unsaturated : saturated acid ratio and fluidity of the membrane.", "PMID": 952983}
{"id": "pubmed23n0032_371", "title": "Concentration changes of bile acids in sequential segments of pigeon intestine and their relation to bile acid absorption.", "content": "Changes in the concentration of bile acids in sequential segments of pigeon intestine were measured. It was found that the jejunum has the highest concentration of bile acids. When the ratio of bile acids to beta-sitosterol (a non-absorbable dietary marker) was examined in contents of various segments, it was found that the ratio showed a marked decrease (86.6%) from the upper to lower jejunum while the subsequent changes in other segments were not as striking. This indicates that the jejunum is the major site of bile acid absorption in the pigeon, unlike in mammals, where the ileum has been shown to be the major site of bile acid absorption. This notion was confirmed by studies of the effect of ileal bypass in these pigeons. This surgical procedure did not significantly change the fecal excretion of bile acids or neutral sterols indicating that the jejunum is capable of maintaining a normal enterohepatic circulation of bile acids in the absence of the ileum in the pigeons.", "contents": "Concentration changes of bile acids in sequential segments of pigeon intestine and their relation to bile acid absorption. Changes in the concentration of bile acids in sequential segments of pigeon intestine were measured. It was found that the jejunum has the highest concentration of bile acids. When the ratio of bile acids to beta-sitosterol (a non-absorbable dietary marker) was examined in contents of various segments, it was found that the ratio showed a marked decrease (86.6%) from the upper to lower jejunum while the subsequent changes in other segments were not as striking. This indicates that the jejunum is the major site of bile acid absorption in the pigeon, unlike in mammals, where the ileum has been shown to be the major site of bile acid absorption. This notion was confirmed by studies of the effect of ileal bypass in these pigeons. This surgical procedure did not significantly change the fecal excretion of bile acids or neutral sterols indicating that the jejunum is capable of maintaining a normal enterohepatic circulation of bile acids in the absence of the ileum in the pigeons.", "PMID": 952984}
{"id": "pubmed23n0032_372", "title": "Effects of local anaesthetics on phospholipases.", "content": "1. The effects of six local anaesthetics have been studied on the activities of soluble phospholipases A2 (EC 3.1.1.4) and lysophospholipase (EC 3.1.1.5). 2. Phospholipase A2 activity in human seminal plasma towards sonicated radioactively-labelled phosphatidylethanolamine was slightly stimulated a low and inhibited at high concentrations of all anaesthetic compounds employed. The order of decreasing potency was chlorpromazine, dibucaine, tetracaine, lidocaine, cocaine and procaine. In line with previous findings, the mode of inhibition was seen to be competitive with respect to Ca2+. 3. Phospholipase A2 activity in crude venom of Crotalus adamanteus was not affected or slightly stimulated by local anaesthetics up to 10(-2) M concentrations, when egg yolk was used as substrate. However, with sonicated radioactively-labelled phosphatidylcholine or phosphatidylethanolamine as substrate, stimulation of phospholipase activity was seen with all local anaesthetics up to 10(-2) M, the order of decreasing potency again being chlorpromazine, dibucaine, tetracaine, lidocaine, cocaine and procaine. The mode of stimulation was seen to be un-competitive with respect to substrate and probably independent of any involvement of Ca2+. 4. As in seminal plasma phospholipase A2, the activity in crude Naja naja venom towards sonicated radioactively labelled phosphatidylcholine was stimulated at low and inhibited at high concentrations of dibucaine and chloropromazine, for example. The mode of inhibition was seen to be competitive with respect to Ca2+, whereas stimulation by the anaesthetic drugs was independent of Ca2+. Binding between drug and enzyme was demonstrated by equilibration filtration of purified phospholipase A2 of Naja naja venom through a Sephadex G 25-fine column, previously equilibrated with 0.5 mM radioactively labelled chlorpromazine. 5. Lysophospholipase activity in rat liver cytosol towards radioactively labelled lysophosphatidylcholine was  inhibited by all local anaesthetics used; the order of decreasing potency was chlorpromazine, dibucaine, tetracaine, cocaine, lidocaine and procaine. The inhibition was un-competitive with respect to substrate. 6. The inhibitory and stimulatory potencies of the local anaesthetics employed closely parallel their lipid solubilities and anaesthetic potencies.", "contents": "Effects of local anaesthetics on phospholipases. 1. The effects of six local anaesthetics have been studied on the activities of soluble phospholipases A2 (EC 3.1.1.4) and lysophospholipase (EC 3.1.1.5). 2. Phospholipase A2 activity in human seminal plasma towards sonicated radioactively-labelled phosphatidylethanolamine was slightly stimulated a low and inhibited at high concentrations of all anaesthetic compounds employed. The order of decreasing potency was chlorpromazine, dibucaine, tetracaine, lidocaine, cocaine and procaine. In line with previous findings, the mode of inhibition was seen to be competitive with respect to Ca2+. 3. Phospholipase A2 activity in crude venom of Crotalus adamanteus was not affected or slightly stimulated by local anaesthetics up to 10(-2) M concentrations, when egg yolk was used as substrate. However, with sonicated radioactively-labelled phosphatidylcholine or phosphatidylethanolamine as substrate, stimulation of phospholipase activity was seen with all local anaesthetics up to 10(-2) M, the order of decreasing potency again being chlorpromazine, dibucaine, tetracaine, lidocaine, cocaine and procaine. The mode of stimulation was seen to be un-competitive with respect to substrate and probably independent of any involvement of Ca2+. 4. As in seminal plasma phospholipase A2, the activity in crude Naja naja venom towards sonicated radioactively labelled phosphatidylcholine was stimulated at low and inhibited at high concentrations of dibucaine and chloropromazine, for example. The mode of inhibition was seen to be competitive with respect to Ca2+, whereas stimulation by the anaesthetic drugs was independent of Ca2+. Binding between drug and enzyme was demonstrated by equilibration filtration of purified phospholipase A2 of Naja naja venom through a Sephadex G 25-fine column, previously equilibrated with 0.5 mM radioactively labelled chlorpromazine. 5. Lysophospholipase activity in rat liver cytosol towards radioactively labelled lysophosphatidylcholine was  inhibited by all local anaesthetics used; the order of decreasing potency was chlorpromazine, dibucaine, tetracaine, cocaine, lidocaine and procaine. The inhibition was un-competitive with respect to substrate. 6. The inhibitory and stimulatory potencies of the local anaesthetics employed closely parallel their lipid solubilities and anaesthetic potencies.", "PMID": 952985}
{"id": "pubmed23n0032_373", "title": "The relationship between chain elongation of palmitoyl-CoA and phospholipid content in rat liver microsomes.", "content": "The relationship between the chain elongation of palmitoyl-CoA and phospholipid content in rat liver microsomes was studied. When liver microsomes were incubated with phospholipase C, microsomal phospholipids were linearly hydrolyzed during 10 min of incubation under the present experimental conditions. Coincident with the decrease in microsomal phospholipid content by phospholipase C treatment, the chain elongation activity also decreased linearly. The decreased chain elongation activity in phospholipase C-treated microsomes was completely or partially recovered by the addition of a sonicated dispersion of phosphatidylcholine, microsomal phospholipids or phosphatidylcholine/phosphatidylethanolamine mixtures. The extent of recovery of decreased activity by a sonicated dispersion of phosphatidylcholine/phosphatidylethanolamine mixture was gradually reduced by increasing amounts of phosphatidylethanolamine in the dispersion. In addition, the chain elongation activity in native nicrosomes was more stimulated by the addition of a sonicated dispersion of phosphatidylcholine alone than by that of phosphatidylcholine/phosphatidylethanolamine mixtures. The chain elongation activity of palmitoyl-CoA was inhibited by the addition of stearoyl-CoA which is the end-product of this reaction. The inhibitory effect of stearoyl-CoA was partially eliminated by the addition of a sonicated dispersion of phosphatidylcholine. The increase of the chain elongation activity in native and phospholipase C-treated microsomes by the addition of a sonicated dispersion of phosphatidylcholine was not related to the activity of fatty acyl-CoA hydrolase.", "contents": "The relationship between chain elongation of palmitoyl-CoA and phospholipid content in rat liver microsomes. The relationship between the chain elongation of palmitoyl-CoA and phospholipid content in rat liver microsomes was studied. When liver microsomes were incubated with phospholipase C, microsomal phospholipids were linearly hydrolyzed during 10 min of incubation under the present experimental conditions. Coincident with the decrease in microsomal phospholipid content by phospholipase C treatment, the chain elongation activity also decreased linearly. The decreased chain elongation activity in phospholipase C-treated microsomes was completely or partially recovered by the addition of a sonicated dispersion of phosphatidylcholine, microsomal phospholipids or phosphatidylcholine/phosphatidylethanolamine mixtures. The extent of recovery of decreased activity by a sonicated dispersion of phosphatidylcholine/phosphatidylethanolamine mixture was gradually reduced by increasing amounts of phosphatidylethanolamine in the dispersion. In addition, the chain elongation activity in native nicrosomes was more stimulated by the addition of a sonicated dispersion of phosphatidylcholine alone than by that of phosphatidylcholine/phosphatidylethanolamine mixtures. The chain elongation activity of palmitoyl-CoA was inhibited by the addition of stearoyl-CoA which is the end-product of this reaction. The inhibitory effect of stearoyl-CoA was partially eliminated by the addition of a sonicated dispersion of phosphatidylcholine. The increase of the chain elongation activity in native and phospholipase C-treated microsomes by the addition of a sonicated dispersion of phosphatidylcholine was not related to the activity of fatty acyl-CoA hydrolase.", "PMID": 952986}
{"id": "pubmed23n0032_374", "title": "Metabolism of 8,11,14-eicosatrienoic acid in human platelets.", "content": "The following labeled compounds were isolated and identified after incubation of 8,11,14-eicosatrien [1-14C] oic acid with human platelets: 12-L-hydroxy-8,10,14-eicosatrienoic acid, 8,11,12-trihydroxy-9,14-eicosadienoic acid, 8,9,12-trihydroxy-10,14-eicosadienoic acid, 12-L-hydroxy-8,10-heptadecadienoic acid, prostaglandin E1, prostaglandin D1, and 8-(1-hydroxy-3-oxopropyl)-9,12-dihydroxy-10-heptadecenoic acid (thromboxane B1).", "contents": "Metabolism of 8,11,14-eicosatrienoic acid in human platelets. The following labeled compounds were isolated and identified after incubation of 8,11,14-eicosatrien [1-14C] oic acid with human platelets: 12-L-hydroxy-8,10,14-eicosatrienoic acid, 8,11,12-trihydroxy-9,14-eicosadienoic acid, 8,9,12-trihydroxy-10,14-eicosadienoic acid, 12-L-hydroxy-8,10-heptadecadienoic acid, prostaglandin E1, prostaglandin D1, and 8-(1-hydroxy-3-oxopropyl)-9,12-dihydroxy-10-heptadecenoic acid (thromboxane B1).", "PMID": 952987}
{"id": "pubmed23n0032_375", "title": "Triacylglycerol contents and in vivo lipogenesis of ob/ob, db/db and Avy/a mice.", "content": "The triacylglycerol content and the in vivo lipogenesis rates of the liver and the carcass of 12 groups of mice were studied. They were mice of three strains and affected by mutations at three loci: C57BL/6J-ob/ob and normal mice; C57BL/KsJ-db/db and normal mice; and VY/WfL-Avy/a and normal mice. Each type of mice was studied at two body weight levels, before and after the mutants became grossly obese. It was found that the C57BL/6J-ob/ob and the C57BL/KsJ-db/db mice had the characteristics of juvenile type obesity. They had higher lipogenesis rates and accumulated more triacylglycerol when they were young. They gained weight rapidly mainly due to the accumulation of more triacylglycerol as they matured. Their total triacylglycerol content could reach 50% of their body weight. At maturity, their lipogenesis rates had decreased to normal. In contrast, the VY/WfL-Avy/a mice had the characteristics of maturity-onset type obesity. When they were young, they did not have higher lipogenesis rates and had only a moderate amount of triacylglycerol stored. They did not gain weight rapidly as they matured. However, when they reached maturity, their lipogenesis rate did not decrease. Their body triacylglycerol content was about 25% of their weight.", "contents": "Triacylglycerol contents and in vivo lipogenesis of ob/ob, db/db and Avy/a mice. The triacylglycerol content and the in vivo lipogenesis rates of the liver and the carcass of 12 groups of mice were studied. They were mice of three strains and affected by mutations at three loci: C57BL/6J-ob/ob and normal mice; C57BL/KsJ-db/db and normal mice; and VY/WfL-Avy/a and normal mice. Each type of mice was studied at two body weight levels, before and after the mutants became grossly obese. It was found that the C57BL/6J-ob/ob and the C57BL/KsJ-db/db mice had the characteristics of juvenile type obesity. They had higher lipogenesis rates and accumulated more triacylglycerol when they were young. They gained weight rapidly mainly due to the accumulation of more triacylglycerol as they matured. Their total triacylglycerol content could reach 50% of their body weight. At maturity, their lipogenesis rates had decreased to normal. In contrast, the VY/WfL-Avy/a mice had the characteristics of maturity-onset type obesity. When they were young, they did not have higher lipogenesis rates and had only a moderate amount of triacylglycerol stored. They did not gain weight rapidly as they matured. However, when they reached maturity, their lipogenesis rate did not decrease. Their body triacylglycerol content was about 25% of their weight.", "PMID": 952988}
{"id": "pubmed23n0032_376", "title": "Studies on lysophospholipases. VIII. Immunochemical differences between two lysophospholipases from beef liver.", "content": "1. Two distinct lysophospholipases have previously been obtained in homogeneous form from beef liver. In this paper, we demonstrate that ageing of a beef liver homogenate does not result in a change in the ratio of the two enzymatic activities, indicating that no interconversion of the lysophospholipases took place. 2. Possible partial structural relationships between the two enzymes were explored by immunochemical techniques. Rabbit antisera raised against each individual lysophospholipase showed no cross-reactivity with the other enzyme. This was concluded from immuno double-diffusion experiments and from the results of immunoprecipitation of enzymatic activities in solution. 3. Lysophospholipase and esterase activity in the purified preparation of lysophospholipase II from beef liver were concomitantly precipitated by anti-serum against lysophospholipase II. This is further proof that both enzymatic activities reside in a single polypeptide chain, in agreement with previous results of isoelectric focusing experiments.", "contents": "Studies on lysophospholipases. VIII. Immunochemical differences between two lysophospholipases from beef liver. 1. Two distinct lysophospholipases have previously been obtained in homogeneous form from beef liver. In this paper, we demonstrate that ageing of a beef liver homogenate does not result in a change in the ratio of the two enzymatic activities, indicating that no interconversion of the lysophospholipases took place. 2. Possible partial structural relationships between the two enzymes were explored by immunochemical techniques. Rabbit antisera raised against each individual lysophospholipase showed no cross-reactivity with the other enzyme. This was concluded from immuno double-diffusion experiments and from the results of immunoprecipitation of enzymatic activities in solution. 3. Lysophospholipase and esterase activity in the purified preparation of lysophospholipase II from beef liver were concomitantly precipitated by anti-serum against lysophospholipase II. This is further proof that both enzymatic activities reside in a single polypeptide chain, in agreement with previous results of isoelectric focusing experiments.", "PMID": 952989}
{"id": "pubmed23n0032_377", "title": "Cholesterol sulfate in rat tissues. Tissue distribution, developmental change and brain subcellular localization.", "content": "1. A reliable micromethod for the determination of the tissue level of cholesterol sulfate has been developed. Cholesterol sulfate was separated from the bulk of the free cholesterol by silica gel column chromatography, and the cholesterol sulfate fraction subjected to benzoylation. A small amount of contaminating free cholesterol and other lipids remaining in this fraction were converted to benzoyl esters while the cholesterol sulfate remained unreacted. The cholesterol sulfate was then separated from the benzoylated contaminants by a second silica gel chromatography column and subjected to solvolysis. The liberated cholesterol was determined by gas-liquid chromatography. 2. The cholesterol sulfate contents of the visceral organs of 43-day-old rats were determined. Every tissue examined contained small amounts of this sulfate. Kidney contained the highest concentration of cholesterol sulfate (250-300 mug/g dry tissue weight) followed by spleen (77 mug/g), adrenal gland (50-70 mug/g) and lung (50-57 mug/g). 3. In brain, cholesterol sulfate level rises sharply from 17 mug/g dry weight in 7-day-old rats to more than 50 mug/g in 15-day-olds, then it declines rapidly to 15 mug/g in the 40-day-olds and this level is maintained to adulthood. The developmental pattern in the liver resembles that in the brain, except that the peak is somewhat flatter with the highest value (60 mug/g dry weight) occurring in the 21-day-old animal. In contrast to the above two tissues, the level of kidney cholesterol sulfate increases steadily from 15 mug/g in 7-day-olds and reaches the adult level of approx. 200 mug/g in 50-day-olds. 4. The highest level of cholesterol sulfate in subcellular fractions of rat brain occurred in a fraction rich in nerve endings. The level here was 10 times higher than that in the mitochondrial fraction, which contained the lowest levels of this steroid sulfate.", "contents": "Cholesterol sulfate in rat tissues. Tissue distribution, developmental change and brain subcellular localization. 1. A reliable micromethod for the determination of the tissue level of cholesterol sulfate has been developed. Cholesterol sulfate was separated from the bulk of the free cholesterol by silica gel column chromatography, and the cholesterol sulfate fraction subjected to benzoylation. A small amount of contaminating free cholesterol and other lipids remaining in this fraction were converted to benzoyl esters while the cholesterol sulfate remained unreacted. The cholesterol sulfate was then separated from the benzoylated contaminants by a second silica gel chromatography column and subjected to solvolysis. The liberated cholesterol was determined by gas-liquid chromatography. 2. The cholesterol sulfate contents of the visceral organs of 43-day-old rats were determined. Every tissue examined contained small amounts of this sulfate. Kidney contained the highest concentration of cholesterol sulfate (250-300 mug/g dry tissue weight) followed by spleen (77 mug/g), adrenal gland (50-70 mug/g) and lung (50-57 mug/g). 3. In brain, cholesterol sulfate level rises sharply from 17 mug/g dry weight in 7-day-old rats to more than 50 mug/g in 15-day-olds, then it declines rapidly to 15 mug/g in the 40-day-olds and this level is maintained to adulthood. The developmental pattern in the liver resembles that in the brain, except that the peak is somewhat flatter with the highest value (60 mug/g dry weight) occurring in the 21-day-old animal. In contrast to the above two tissues, the level of kidney cholesterol sulfate increases steadily from 15 mug/g in 7-day-olds and reaches the adult level of approx. 200 mug/g in 50-day-olds. 4. The highest level of cholesterol sulfate in subcellular fractions of rat brain occurred in a fraction rich in nerve endings. The level here was 10 times higher than that in the mitochondrial fraction, which contained the lowest levels of this steroid sulfate.", "PMID": 952990}
{"id": "pubmed23n0032_378", "title": "The effect of portacaval anastomosis on plasma lipoprotein metabolism in rats.", "content": "1. The effect of portacaval anastomosis on the metabolism of plasma lipoproteins was investigated in rats. 2. When compared with sham-operated pair-fed controls, plasma high density lipoprotein cholesterol concentration was decreased, plasma low density lipoprotein cholesterol concentration was increased and plasma total cholesterol concentration was unchanged in the portacaval anastomosis rats. Maximal incorporation of [14C]leucine into the total circulating mass of protein was decreased in the very low density lipoprotein and high density lipoprotein fractions and, possibly, in the low density lipoprotein fraction, but there was no change in maximal incorporation into albumin. It is concluded that portacaval anastomosis diminishes the rate of synthesis of high density lipoprotein and very low density lipoprotein proteins and, possibly, of low density lipoprotein proteins.", "contents": "The effect of portacaval anastomosis on plasma lipoprotein metabolism in rats. 1. The effect of portacaval anastomosis on the metabolism of plasma lipoproteins was investigated in rats. 2. When compared with sham-operated pair-fed controls, plasma high density lipoprotein cholesterol concentration was decreased, plasma low density lipoprotein cholesterol concentration was increased and plasma total cholesterol concentration was unchanged in the portacaval anastomosis rats. Maximal incorporation of [14C]leucine into the total circulating mass of protein was decreased in the very low density lipoprotein and high density lipoprotein fractions and, possibly, in the low density lipoprotein fraction, but there was no change in maximal incorporation into albumin. It is concluded that portacaval anastomosis diminishes the rate of synthesis of high density lipoprotein and very low density lipoprotein proteins and, possibly, of low density lipoprotein proteins.", "PMID": 952991}
{"id": "pubmed23n0032_379", "title": "The glycosphingolipids of rat sublingual and submaxillary glands.", "content": "1. Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialo-and disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.", "contents": "The glycosphingolipids of rat sublingual and submaxillary glands. 1. Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialo-and disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.", "PMID": 952992}
{"id": "pubmed23n0032_380", "title": "[Characterisation of D(-)glyceric acid from Nocardia caviae (author's transl)].", "content": "A glycolipid was found in a strain of Nocardia caviae. It consists of glucose, myristic, palmitic and stearic acids and a polyhydroxylated acid. The structure of this hydroxyacid was demonstrated by the identification of the product glycerol after LiA1H4 reduction of the glycolipid methyl ester and subsequent hydrolysis, by comparison of the infrared spectra of the hydroxyacid and glyceric acid, by gas chromatography of acetylated methyl and ethyl esters of the polyhydroxylated acid and of standard glyceric acid and by mass spectrometry of the diacetylated methyl ester. The hydroxyacid from the glycolipid is D(-)glyceric acid, a compound rarely found amongst natural products.", "contents": "[Characterisation of D(-)glyceric acid from Nocardia caviae (author's transl)]. A glycolipid was found in a strain of Nocardia caviae. It consists of glucose, myristic, palmitic and stearic acids and a polyhydroxylated acid. The structure of this hydroxyacid was demonstrated by the identification of the product glycerol after LiA1H4 reduction of the glycolipid methyl ester and subsequent hydrolysis, by comparison of the infrared spectra of the hydroxyacid and glyceric acid, by gas chromatography of acetylated methyl and ethyl esters of the polyhydroxylated acid and of standard glyceric acid and by mass spectrometry of the diacetylated methyl ester. The hydroxyacid from the glycolipid is D(-)glyceric acid, a compound rarely found amongst natural products.", "PMID": 952993}
{"id": "pubmed23n0032_381", "title": "Abnormal activation of sterol synthesis in lymphocytes of atherosclerotic-susceptible pigeons and heterozygous familial hypercholesterolemic patients.", "content": "Delipidated human sera enhances the incorporation of [2-14C]acetate, but not mevalonate, into digitonin-precipitable sterols of pigeon lymphocytes. Show Racer and White Carneau pigeons exhibit inherited differences in induction of sterol synthesis dissociated from inheritance of hypercholesterolemia. Moreover, lymphocytes of three familial hypercholesterolemic individuals, having serum cholesterol level in the normal range by drug therapy, exhibited a higher activation of sterol synthesis by delipidated sera when compared to cells of normal individuals. It is suggested that genetic abnormality in lymphocytes of familial hypercholesterolemic can be dissociated from hypercholesterolemia.", "contents": "Abnormal activation of sterol synthesis in lymphocytes of atherosclerotic-susceptible pigeons and heterozygous familial hypercholesterolemic patients. Delipidated human sera enhances the incorporation of [2-14C]acetate, but not mevalonate, into digitonin-precipitable sterols of pigeon lymphocytes. Show Racer and White Carneau pigeons exhibit inherited differences in induction of sterol synthesis dissociated from inheritance of hypercholesterolemia. Moreover, lymphocytes of three familial hypercholesterolemic individuals, having serum cholesterol level in the normal range by drug therapy, exhibited a higher activation of sterol synthesis by delipidated sera when compared to cells of normal individuals. It is suggested that genetic abnormality in lymphocytes of familial hypercholesterolemic can be dissociated from hypercholesterolemia.", "PMID": 952994}
{"id": "pubmed23n0032_382", "title": "Metabolism of delta7- and delta5,7-sterols by Phytophthora cactorum.", "content": "delta7- and delta5,7-Sterols are converted to the corresponding delta5-sterols by Phytophthora cactorum. When delta7-sterols were supplied to the fungus, delta5- and delta7-sterols were recovered in an acetone extract of the mycelium, the proportion of delta5-sterol being higher in the free sterol fraction than in the esters. When delta5,7-sterols were supplied, delta5-sterols were recovered. No delta5,7-sterols were found in the mycelium. The initial rate of uptake of the sterols was in the order delta5 greater than delta7 greater than delta5,7.", "contents": "Metabolism of delta7- and delta5,7-sterols by Phytophthora cactorum. delta7- and delta5,7-Sterols are converted to the corresponding delta5-sterols by Phytophthora cactorum. When delta7-sterols were supplied to the fungus, delta5- and delta7-sterols were recovered in an acetone extract of the mycelium, the proportion of delta5-sterol being higher in the free sterol fraction than in the esters. When delta5,7-sterols were supplied, delta5-sterols were recovered. No delta5,7-sterols were found in the mycelium. The initial rate of uptake of the sterols was in the order delta5 greater than delta7 greater than delta5,7.", "PMID": 952995}
{"id": "pubmed23n0032_383", "title": "Isolation and characterization of insect vitellogenin. Its identity with hemolymph lipoprotein II.", "content": "Several years ago, we isolated two major lipoproteins from the pupal hemolymph of the Cynthia silkworm, both of which contained diacylglycerol as a major lipid, and named them lipoproteins I and II. In this paper, we present definite evidence that the vitellogenin (female protein) of this insect is indeed identical with lipoprotein II.", "contents": "Isolation and characterization of insect vitellogenin. Its identity with hemolymph lipoprotein II. Several years ago, we isolated two major lipoproteins from the pupal hemolymph of the Cynthia silkworm, both of which contained diacylglycerol as a major lipid, and named them lipoproteins I and II. In this paper, we present definite evidence that the vitellogenin (female protein) of this insect is indeed identical with lipoprotein II.", "PMID": 952996}
{"id": "pubmed23n0032_384", "title": "Lesions in post-ribosomal supernatant fractions associated with loss of viability in pea (Pisum arvense) seed.", "content": "RNA synthesis and protein synthesis in viable pea embryonic axis tissue commences during the first hour of water imbibition whilst DNA synthesis commences after 8 h of imbibition. Neither DNA synthesis nor protein synthesis could be detected in non-viable axis tissue during the first 24 h of imbibition but some RNA synthesis is detectable during this period. Both post-ribosomal supernatant and ribosomal fractions from imbibed non-viable embryonic axis tissue were impaired in their ability to support polyphenylalanine synthesis in a cell-free protein-synthesising system, yet the same fractions isolated from unimbibed non-viable axis tissue were as efficient as equivalent fractions from unimbibed viable axis tissue in the support of polyphenylalanine synthesis in the cell-free system. A major lesion in elongation factor 1 activity and additional lesions in elongation factor 2 and phenylalanyl-tRNA synthetase activities were detected in the post-ribosomal supernatants isolated from non-viable embryonic axis tissue.", "contents": "Lesions in post-ribosomal supernatant fractions associated with loss of viability in pea (Pisum arvense) seed. RNA synthesis and protein synthesis in viable pea embryonic axis tissue commences during the first hour of water imbibition whilst DNA synthesis commences after 8 h of imbibition. Neither DNA synthesis nor protein synthesis could be detected in non-viable axis tissue during the first 24 h of imbibition but some RNA synthesis is detectable during this period. Both post-ribosomal supernatant and ribosomal fractions from imbibed non-viable embryonic axis tissue were impaired in their ability to support polyphenylalanine synthesis in a cell-free protein-synthesising system, yet the same fractions isolated from unimbibed non-viable axis tissue were as efficient as equivalent fractions from unimbibed viable axis tissue in the support of polyphenylalanine synthesis in the cell-free system. A major lesion in elongation factor 1 activity and additional lesions in elongation factor 2 and phenylalanyl-tRNA synthetase activities were detected in the post-ribosomal supernatants isolated from non-viable embryonic axis tissue.", "PMID": 952997}
{"id": "pubmed23n0032_385", "title": "Ion binding and chromatin condensation.", "content": "1. The binding of 45Ca2+ to hen erythrocyte chromatin has been studied to help elucidate how cations induce a reversible condensation of this chromatin. 2. As the unbound Ca2+ of the medium rises from 0.5 to 4 mM, Ca2+ is bound to the chromatin with a stability constant of approx. 3.1 and a saturation value of 0.25 Ca2+ per DNA phosphate, or one-half the value for pure DNA. Condensation of the chromatin is half complete when this binding of calcium is roughly half complete. Hence the transition from the uncondensed to the condensed state occurs as repulsion between the free DNA phosphates of erythrocyte chromatin is neutralised by bound cations. Genetically active chromatin may be maintained in an uncondensed state in living cells by the presence of different negative groups that remain unneutralised at the unbound cation concentrations of the cell. 3. That only one-half of the calcium binding sites of DNA are masked in erythrocyte chromatin supports recent models of chromatin structure in which the DNA double helix is wound round a core of histones. 4. Competition for calcium binding sites in the chromatin by other cations was also studied.", "contents": "Ion binding and chromatin condensation. 1. The binding of 45Ca2+ to hen erythrocyte chromatin has been studied to help elucidate how cations induce a reversible condensation of this chromatin. 2. As the unbound Ca2+ of the medium rises from 0.5 to 4 mM, Ca2+ is bound to the chromatin with a stability constant of approx. 3.1 and a saturation value of 0.25 Ca2+ per DNA phosphate, or one-half the value for pure DNA. Condensation of the chromatin is half complete when this binding of calcium is roughly half complete. Hence the transition from the uncondensed to the condensed state occurs as repulsion between the free DNA phosphates of erythrocyte chromatin is neutralised by bound cations. Genetically active chromatin may be maintained in an uncondensed state in living cells by the presence of different negative groups that remain unneutralised at the unbound cation concentrations of the cell. 3. That only one-half of the calcium binding sites of DNA are masked in erythrocyte chromatin supports recent models of chromatin structure in which the DNA double helix is wound round a core of histones. 4. Competition for calcium binding sites in the chromatin by other cations was also studied.", "PMID": 952998}
{"id": "pubmed23n0032_386", "title": "DNA uptake by Streptomyces species.", "content": "Uptake of homologous and heterologous radioactively labelled DNA was studied in ten Streptomyces species. Among these, S. kasugaensis and S. virginiae were shown to take up 0.5-2.0% of the supplied donor DNA at a well-defined point in their growth cycle. The heterologous donor DNA taken up retained its original buoyant density in CsCl gradients. Other strains either extensively degraded the donor DNA or showed poor uptake efficiency.", "contents": "DNA uptake by Streptomyces species. Uptake of homologous and heterologous radioactively labelled DNA was studied in ten Streptomyces species. Among these, S. kasugaensis and S. virginiae were shown to take up 0.5-2.0% of the supplied donor DNA at a well-defined point in their growth cycle. The heterologous donor DNA taken up retained its original buoyant density in CsCl gradients. Other strains either extensively degraded the donor DNA or showed poor uptake efficiency.", "PMID": 952999}
{"id": "pubmed23n0032_387", "title": "Lesions in the ribosomes of non-viable pea (Pisum arvense) embryonic axis tissue.", "content": "Ribosomes isolated from either dry viable or non-viable pea embryonic axis tissue were equally effective in the support of polyphenylalanine synthesis in a poly(U)-directed cell-free protein-synthesising system. Ribosomes isolated from imbibed non-viable axis tissue were impaired in their ability to support polyphenylalanine synthesis in the cell-free system. RNA isolated from ribosomes and 40-S ribosomal subunits of dry or imbibed viable axis tissue was found not to be degraded, whereas the equivalent RNA species isolated from non-viable axis tissue showed an increased degree of breakdown as imbibition proceeded. Even though rRNA of imbibed non-viable axis tissue was degraded, the ribosomes and ribosomal subunits of these embryos appeared intact. In viable embryonic axis tissue the percentage of ribosomes present in the cell in the form of polysomes increased during imbibition whereas no polysomes could be detected in ribosomal preparations from dry or imbibed non-viable axis tissue. The breakdown of rRNA in ribosomal particles from non-viable axis tissue may be a contributory factor to senescence and loss of viability in Pisum arvense.", "contents": "Lesions in the ribosomes of non-viable pea (Pisum arvense) embryonic axis tissue. Ribosomes isolated from either dry viable or non-viable pea embryonic axis tissue were equally effective in the support of polyphenylalanine synthesis in a poly(U)-directed cell-free protein-synthesising system. Ribosomes isolated from imbibed non-viable axis tissue were impaired in their ability to support polyphenylalanine synthesis in the cell-free system. RNA isolated from ribosomes and 40-S ribosomal subunits of dry or imbibed viable axis tissue was found not to be degraded, whereas the equivalent RNA species isolated from non-viable axis tissue showed an increased degree of breakdown as imbibition proceeded. Even though rRNA of imbibed non-viable axis tissue was degraded, the ribosomes and ribosomal subunits of these embryos appeared intact. In viable embryonic axis tissue the percentage of ribosomes present in the cell in the form of polysomes increased during imbibition whereas no polysomes could be detected in ribosomal preparations from dry or imbibed non-viable axis tissue. The breakdown of rRNA in ribosomal particles from non-viable axis tissue may be a contributory factor to senescence and loss of viability in Pisum arvense.", "PMID": 953000}
{"id": "pubmed23n0032_388", "title": "Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial tRNAsPro and tRNAsLys by homologous and heterologous enzymes.", "content": "The cytoplasmic prolyl-tRNA synthetase can be separated by hydroxyapatite chromatography, from the enzyme present in the chloroplasts and in the mitochondria (organellar enzyme). The cytoplasmic lysyl-tRNA synthetase can also be separated from the organellar enzyme. There are two tRNAsPro in the cytoplasm; they can be charged by the cytoplasmic enzyme, but not by the organellar enzyme or the Escherichia coli enzyme. Chloroplasts contain, in addition to the two cytoplasmic tRNAsPro, one chloroplast-specific tRNAPro, which is not recognized by the cytoplasmic enzyme, but can be charged by the organellar or the E. coli enzyme. Mitochondria contain, in addition to the two cytoplasmic tRNAsPro, two mitochondria-specific tRNAsPro, which are not recognized by the cytoplasmic enzyme, but can be charged by the organellar or the E. coli enzyme. There are two tRNAsLys in the cytoplasm. Both can be charged by the cytoplasmic enzyme, but one can be charged by the organellar or E. coli enzyme. Chloroplasts contain in addition to one cytoplasmic tRNALys, one chloroplast-specific tRNALys which can only be charged by the organellar or E. coli enzyme. Mitochondria contain, in addition to one cytoplasmic tRNALys, one mitochondria-specific tRNALys which can only be charged by the organellar or E. coli enzyme.", "contents": "Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial tRNAsPro and tRNAsLys by homologous and heterologous enzymes. The cytoplasmic prolyl-tRNA synthetase can be separated by hydroxyapatite chromatography, from the enzyme present in the chloroplasts and in the mitochondria (organellar enzyme). The cytoplasmic lysyl-tRNA synthetase can also be separated from the organellar enzyme. There are two tRNAsPro in the cytoplasm; they can be charged by the cytoplasmic enzyme, but not by the organellar enzyme or the Escherichia coli enzyme. Chloroplasts contain, in addition to the two cytoplasmic tRNAsPro, one chloroplast-specific tRNAPro, which is not recognized by the cytoplasmic enzyme, but can be charged by the organellar or the E. coli enzyme. Mitochondria contain, in addition to the two cytoplasmic tRNAsPro, two mitochondria-specific tRNAsPro, which are not recognized by the cytoplasmic enzyme, but can be charged by the organellar or the E. coli enzyme. There are two tRNAsLys in the cytoplasm. Both can be charged by the cytoplasmic enzyme, but one can be charged by the organellar or E. coli enzyme. Chloroplasts contain in addition to one cytoplasmic tRNALys, one chloroplast-specific tRNALys which can only be charged by the organellar or E. coli enzyme. Mitochondria contain, in addition to one cytoplasmic tRNALys, one mitochondria-specific tRNALys which can only be charged by the organellar or E. coli enzyme.", "PMID": 953001}
{"id": "pubmed23n0032_389", "title": "The buoyant density of three double-stranded RNAs in cesium sulfate.", "content": "The buoyant densities in Cs2SO4 of the double-stranded RNA from the bacteriophage phi 6 and the virus-like particles of Helminthosporium maydis and Penicillium chrysogenum were determined by a Taylor series expansion and by the position and slope of the gradient relative to the isopycnic position (hinge point method). Buoyant densities for the three types of double-stranded RNA calculated by the two methods were, respectively, 1.6089 and 1.6083, 1.6065 and 1.6059 and 1.6057 and 1.6050.", "contents": "The buoyant density of three double-stranded RNAs in cesium sulfate. The buoyant densities in Cs2SO4 of the double-stranded RNA from the bacteriophage phi 6 and the virus-like particles of Helminthosporium maydis and Penicillium chrysogenum were determined by a Taylor series expansion and by the position and slope of the gradient relative to the isopycnic position (hinge point method). Buoyant densities for the three types of double-stranded RNA calculated by the two methods were, respectively, 1.6089 and 1.6083, 1.6065 and 1.6059 and 1.6057 and 1.6050.", "PMID": 953002}
{"id": "pubmed23n0032_390", "title": "Newly synthesized mammalian cell DNA. Separation of Okazaki pieces by thermal chromatography on hydroxyapatite.", "content": "The optical melting profile of a fraction of nascent DNA which can be isolated from DNA of logarithmically growing Ehrlich ascites cells by nitrocellullose column chromatography was recorded. In relation to the bulk DNA a slightly lowered Tm and an increased width of the transition was observed. Thermal hydroxyapatite chromatography revealed that melting of the nascent DNA begins at relatively low temperatures in helix regions containing small daughter strand pieces. The pieces were characterized as Okazaki pieces by determining their size and their labeling kinetics during [3H] thymidine pulses. The combination of nitrocellulose chromatography and thermal hydroxyapatite chromatography seems to be suitable for isolating Okazaki pieces.", "contents": "Newly synthesized mammalian cell DNA. Separation of Okazaki pieces by thermal chromatography on hydroxyapatite. The optical melting profile of a fraction of nascent DNA which can be isolated from DNA of logarithmically growing Ehrlich ascites cells by nitrocellullose column chromatography was recorded. In relation to the bulk DNA a slightly lowered Tm and an increased width of the transition was observed. Thermal hydroxyapatite chromatography revealed that melting of the nascent DNA begins at relatively low temperatures in helix regions containing small daughter strand pieces. The pieces were characterized as Okazaki pieces by determining their size and their labeling kinetics during [3H] thymidine pulses. The combination of nitrocellulose chromatography and thermal hydroxyapatite chromatography seems to be suitable for isolating Okazaki pieces.", "PMID": 953003}
{"id": "pubmed23n0032_391", "title": "NMR studies of the structure and stability of the 1 : 2 actinomycin D-d-pG-C complex in aqueous solution.", "content": "We describe NMR studies at superconducting fields which characterize aspects of the structure and stability of the 1 : 2 actinomycin-d-pG-C complex in solution as monitored at the Watson-Crick base pairs and backbone phosphate groups. Two guanine N1H resonances (12.17 and 11.66 ppm) are observed in the 360 MHz proton NMR spectra of the complex in water at -4 degrees C. These slowly exchangeable resonances, which demonstrate the presence of two Watson-Crick G + C base pairs in the complex, broaden in a sequential manner with increasing temperature. The terminal and internucleotide phosphates of both d-pG-C molecules are observable in the 145.7 MHz 31P spectra of the 1 : 2 actinomycin-d-pG-C complex at 0 degrees C. The internucleotide phosphate resonance at 1.905 ppm broadens prior to that at 2.385 ppm with increasing temperature, consistent with a sequential breakage of the G + C base pairs in the complex. The lifetime of the complex (4.5 +/- 0.5 X 10(-4) s, 33 degrees C) was deduced from the variation of the d-pG-D internucleotide 31P resonance line width on gradual addition of the antibiotic in solution.", "contents": "NMR studies of the structure and stability of the 1 : 2 actinomycin D-d-pG-C complex in aqueous solution. We describe NMR studies at superconducting fields which characterize aspects of the structure and stability of the 1 : 2 actinomycin-d-pG-C complex in solution as monitored at the Watson-Crick base pairs and backbone phosphate groups. Two guanine N1H resonances (12.17 and 11.66 ppm) are observed in the 360 MHz proton NMR spectra of the complex in water at -4 degrees C. These slowly exchangeable resonances, which demonstrate the presence of two Watson-Crick G + C base pairs in the complex, broaden in a sequential manner with increasing temperature. The terminal and internucleotide phosphates of both d-pG-C molecules are observable in the 145.7 MHz 31P spectra of the 1 : 2 actinomycin-d-pG-C complex at 0 degrees C. The internucleotide phosphate resonance at 1.905 ppm broadens prior to that at 2.385 ppm with increasing temperature, consistent with a sequential breakage of the G + C base pairs in the complex. The lifetime of the complex (4.5 +/- 0.5 X 10(-4) s, 33 degrees C) was deduced from the variation of the d-pG-D internucleotide 31P resonance line width on gradual addition of the antibiotic in solution.", "PMID": 953004}
{"id": "pubmed23n0032_392", "title": "A sedimentation velocity method for the separation of complementary strands of DNA.", "content": "A method is described for the rapid separation of the complementary strands of homogeneous DNA's. The method takes advantage of a difference in sedimentation coefficients between the complementary strands when these have been dissociated and complexed with poly(U-G). The separation procedure is readily carried out for preparative purposes by zone sedimentation in linear 5--20% (w/v) sucrose gradients containing 2 M NaCl. In analytical band sedimentation experiments, concentrated solutions of CsCl have been used to effect the separation of the complexed complementary strands. Sedimentation coefficients corresponding to corrected values at infinite dilution for the cesium salt of the nucleic acid complexes at 25 degrees C (s 25, w) have been obtained for the complexed complementary strands of coliphage lambdab2b5c DNA and for the uncomplexed single strands of this DNA. The relative values of the parameters affecting s 25, w have been determined. Under these conditions, for which preferential hydration of the sedimenting species is not involved, it was found that the more highly complexed strand has sustained a 24% increase in mass and a 28% decrease in frictional coefficient relative to uncomplexed DNA, while the strand binding the lesser amount of RNA has increased in mass by 11% and changed in frictional coefficient by an insignificant amount relative to the uncomplexed species from that of the uncomplexed DNA do not contribute significantly to the differences in s 25, w between the complexed complementary strands.", "contents": "A sedimentation velocity method for the separation of complementary strands of DNA. A method is described for the rapid separation of the complementary strands of homogeneous DNA's. The method takes advantage of a difference in sedimentation coefficients between the complementary strands when these have been dissociated and complexed with poly(U-G). The separation procedure is readily carried out for preparative purposes by zone sedimentation in linear 5--20% (w/v) sucrose gradients containing 2 M NaCl. In analytical band sedimentation experiments, concentrated solutions of CsCl have been used to effect the separation of the complexed complementary strands. Sedimentation coefficients corresponding to corrected values at infinite dilution for the cesium salt of the nucleic acid complexes at 25 degrees C (s 25, w) have been obtained for the complexed complementary strands of coliphage lambdab2b5c DNA and for the uncomplexed single strands of this DNA. The relative values of the parameters affecting s 25, w have been determined. Under these conditions, for which preferential hydration of the sedimenting species is not involved, it was found that the more highly complexed strand has sustained a 24% increase in mass and a 28% decrease in frictional coefficient relative to uncomplexed DNA, while the strand binding the lesser amount of RNA has increased in mass by 11% and changed in frictional coefficient by an insignificant amount relative to the uncomplexed species from that of the uncomplexed DNA do not contribute significantly to the differences in s 25, w between the complexed complementary strands.", "PMID": 953005}
{"id": "pubmed23n0032_393", "title": "Differentiation between chelated and non-chelated DNA-Pt complexes by atomic absorption spectrophotometry.", "content": "As a continuation of our study of the properties of the cis-trans platinum series we have investigated the DNA-Pt complexes of [Pt(dien)Cl]Cl, cis-Pt (en)Cl2, cis-Pt (NH3)2Cl2 and K2[PtCl4] by atomic absorption spectrophotometry. The DNA-Pt complexes correspond to the saturation of the N7-(Guanine) sites. It has been found that the chelate complexes obtained with cis-Pt(en)Cl2, cis-Pt(NH3)2Cl1 and K2[PtCl4] show the same absorbance. The [Pt(dien)Cl]Cl and trans-Pt (NH3)2Cl2 which are bound to the N7  (tuanine) site only, show an absorbance greater than the chelate complexes by a factor of two. In addition, it has been possible in the case of the trans-Pt (NH3)CCl1 complex to follow the fixation of platinum to DNA by atomic absorption spectrophotometry. The result is similar to the ionic chlorine liberation procedure reported previously.", "contents": "Differentiation between chelated and non-chelated DNA-Pt complexes by atomic absorption spectrophotometry. As a continuation of our study of the properties of the cis-trans platinum series we have investigated the DNA-Pt complexes of [Pt(dien)Cl]Cl, cis-Pt (en)Cl2, cis-Pt (NH3)2Cl2 and K2[PtCl4] by atomic absorption spectrophotometry. The DNA-Pt complexes correspond to the saturation of the N7-(Guanine) sites. It has been found that the chelate complexes obtained with cis-Pt(en)Cl2, cis-Pt(NH3)2Cl1 and K2[PtCl4] show the same absorbance. The [Pt(dien)Cl]Cl and trans-Pt (NH3)2Cl2 which are bound to the N7  (tuanine) site only, show an absorbance greater than the chelate complexes by a factor of two. In addition, it has been possible in the case of the trans-Pt (NH3)CCl1 complex to follow the fixation of platinum to DNA by atomic absorption spectrophotometry. The result is similar to the ionic chlorine liberation procedure reported previously.", "PMID": 953006}
{"id": "pubmed23n0032_394", "title": "Colony-forming ability of ultraviolet-irradiated xeroderma pigmentosum fibroblasts from different DNA repair complementation groups.", "content": "Patients with xeroderma pigmentosum develop severe sunlight-induced damage, including malignant neoplasms, on sun-exposed skin. Some patients also have neurological abnormalities. Xeroderma pigmentosum cells are known to have impaired ability to repair ultraviolet light- or chemical mutagen-induced damage to their DNA, and cell-fusion studies have shown five complementation groups among the DNA excision repair-deficient strains. All xeroderma pigmentosum fibroblast strains we tested had lower colony-forming abilities after ultraviolet irradiation than normal strains. Furthermore, we have found that strains from different complementation groups can have different post-ultraviolet colony-forming abilities and that strains from patients with neurological abnormalities are the most sensitive to ultraviolet light. These results suggest that extremely ineffective repair of damaged DNA in central nervous system neurons may be the cause of the neurological abnormalities.", "contents": "Colony-forming ability of ultraviolet-irradiated xeroderma pigmentosum fibroblasts from different DNA repair complementation groups. Patients with xeroderma pigmentosum develop severe sunlight-induced damage, including malignant neoplasms, on sun-exposed skin. Some patients also have neurological abnormalities. Xeroderma pigmentosum cells are known to have impaired ability to repair ultraviolet light- or chemical mutagen-induced damage to their DNA, and cell-fusion studies have shown five complementation groups among the DNA excision repair-deficient strains. All xeroderma pigmentosum fibroblast strains we tested had lower colony-forming abilities after ultraviolet irradiation than normal strains. Furthermore, we have found that strains from different complementation groups can have different post-ultraviolet colony-forming abilities and that strains from patients with neurological abnormalities are the most sensitive to ultraviolet light. These results suggest that extremely ineffective repair of damaged DNA in central nervous system neurons may be the cause of the neurological abnormalities.", "PMID": 953007}
{"id": "pubmed23n0032_395", "title": "Mechanism of D-amphetamine inhibition of protein synthesis.", "content": "At 1 h after intraperitoneal administration of D-amphetamine sulphate (15 mg/kg), rat brain polyribosomes show disaggregation accompanied by reduced capacity for in vitro peptide chain elongation. The direct action of amphetamine on cell-fine protein-synthesizing systems was therefore explored. When brain or liver polyribosomes from untreated rats were incubated with pH 5 enzyme, peptide chain elongation was not inhibited by the addition 4 mM amphetamine to the  medium. On the other hand, an initiation-dependent system consisting of rat liver of brain mRNA and wheat germ S-30 fraction showed inhibition of [3H]leucine incorporation by 50% when 4 mM amphetamine were added. The metabolites of amphetamine, p-hydroxyamphetamine and p-hydroxynorephedrine, had no inhibitory action in either system, but the potent neurotoxin p-chloroamphetamine was a more powerful inhibitor of initiation than amphetamine. By using [3H]amphetamine, it was shown that amphetamine binds to the 80-S ribosomes of the wheat germ system. This binding depended on the presence in the system of natural liver or brain mRNA or several synthetic mRNAs, but was not promoted by polyuridylic acid as the messenger. Significantly, polyuridylic acid-dependent polyphenylalanine synthesis by the wheat germ system was not inhibited by amphetamine or p-chloroamphetamine. Therefore, it was concluded that amphetamine inhibits protein synthesis by interfering with initiation through a step related to formation of the mRNA ribosome complex.", "contents": "Mechanism of D-amphetamine inhibition of protein synthesis. At 1 h after intraperitoneal administration of D-amphetamine sulphate (15 mg/kg), rat brain polyribosomes show disaggregation accompanied by reduced capacity for in vitro peptide chain elongation. The direct action of amphetamine on cell-fine protein-synthesizing systems was therefore explored. When brain or liver polyribosomes from untreated rats were incubated with pH 5 enzyme, peptide chain elongation was not inhibited by the addition 4 mM amphetamine to the  medium. On the other hand, an initiation-dependent system consisting of rat liver of brain mRNA and wheat germ S-30 fraction showed inhibition of [3H]leucine incorporation by 50% when 4 mM amphetamine were added. The metabolites of amphetamine, p-hydroxyamphetamine and p-hydroxynorephedrine, had no inhibitory action in either system, but the potent neurotoxin p-chloroamphetamine was a more powerful inhibitor of initiation than amphetamine. By using [3H]amphetamine, it was shown that amphetamine binds to the 80-S ribosomes of the wheat germ system. This binding depended on the presence in the system of natural liver or brain mRNA or several synthetic mRNAs, but was not promoted by polyuridylic acid as the messenger. Significantly, polyuridylic acid-dependent polyphenylalanine synthesis by the wheat germ system was not inhibited by amphetamine or p-chloroamphetamine. Therefore, it was concluded that amphetamine inhibits protein synthesis by interfering with initiation through a step related to formation of the mRNA ribosome complex.", "PMID": 953008}
{"id": "pubmed23n0032_396", "title": "Model membrane studies of spin-label probes. Part 1. Mixed monolayers of 12-nitroxide stearic acid and myristic acid.", "content": "Pure and mixed monomolecular films of a cell membrane spin label probe, 12-nitroxide stearic acid have been studied where myristic acid was selected as the host lipid. The behavior of 12-nitroxide stearic acid at the air water interface is understood in terms of two molecular configurations: erect (with only the carboxyl group in the interface) and bent (with both the carboxyl group and the oxazolidine ring in the interface). In mixed films both of these conformations play a role at high surface pressures. At low probe concentrations, 12-nitroxide stearic acid is primarily in an erect conformation, while at high probe concentrations the reverse is true. This particular host lipid appears capable of erecting the probe molecule with only small concentrations of myristic acid. In a condensed host lipid, the probe is partially immiscible, and segregates to form a heterogeneous film from which it is readily collapsed. The probe is seen to perturb the molecular packing in this mixed system and the perturbation to be dependent on both the molecular shape and nature of the probe.", "contents": "Model membrane studies of spin-label probes. Part 1. Mixed monolayers of 12-nitroxide stearic acid and myristic acid. Pure and mixed monomolecular films of a cell membrane spin label probe, 12-nitroxide stearic acid have been studied where myristic acid was selected as the host lipid. The behavior of 12-nitroxide stearic acid at the air water interface is understood in terms of two molecular configurations: erect (with only the carboxyl group in the interface) and bent (with both the carboxyl group and the oxazolidine ring in the interface). In mixed films both of these conformations play a role at high surface pressures. At low probe concentrations, 12-nitroxide stearic acid is primarily in an erect conformation, while at high probe concentrations the reverse is true. This particular host lipid appears capable of erecting the probe molecule with only small concentrations of myristic acid. In a condensed host lipid, the probe is partially immiscible, and segregates to form a heterogeneous film from which it is readily collapsed. The probe is seen to perturb the molecular packing in this mixed system and the perturbation to be dependent on both the molecular shape and nature of the probe.", "PMID": 953009}
{"id": "pubmed23n0032_397", "title": "Evidence for a sodium ion exchange carrier linked with glucose transport across the brush border of a flatworm (Hymenolepis diminuta, Cestoda).", "content": "The manner in which the flatworm, Hymenolepis diminuta (Cestoda), regulates the transport of glucose and Na+ across the brush border was examined. While the presence of an unstirred region in the brush border may favor the reabsorption of leaked glucose, some leaked glucose was lost to the ambient medium. This loss was markedly enhanced by preloading the worms with glucose and by removing Na+ from the incubation medium. Since glucose and Na+ influxes are coupled, glucose leakage stimulated the influx of 22Na+. However, this 22Na+ influx was balanced by a simultaneous increased 22Na+ efflux. The presence of phlorizin inhibited both unidirectional fluxes of 22Na+ indicating that efflux of 22Na+ occurred by countertransport; countertransport of [14C] glucose appeared to be negligible. A model has been proposed in which the transport of glucose and compensating transfers of Na+ across the membrane occur via the same carrier.", "contents": "Evidence for a sodium ion exchange carrier linked with glucose transport across the brush border of a flatworm (Hymenolepis diminuta, Cestoda). The manner in which the flatworm, Hymenolepis diminuta (Cestoda), regulates the transport of glucose and Na+ across the brush border was examined. While the presence of an unstirred region in the brush border may favor the reabsorption of leaked glucose, some leaked glucose was lost to the ambient medium. This loss was markedly enhanced by preloading the worms with glucose and by removing Na+ from the incubation medium. Since glucose and Na+ influxes are coupled, glucose leakage stimulated the influx of 22Na+. However, this 22Na+ influx was balanced by a simultaneous increased 22Na+ efflux. The presence of phlorizin inhibited both unidirectional fluxes of 22Na+ indicating that efflux of 22Na+ occurred by countertransport; countertransport of [14C] glucose appeared to be negligible. A model has been proposed in which the transport of glucose and compensating transfers of Na+ across the membrane occur via the same carrier.", "PMID": 953010}
{"id": "pubmed23n0032_398", "title": "Freeze-fracture electron microscopy of preexisting and nascent cell membrane in cleaving eggs of Xenopus laevis.", "content": "During cell division in the Xenopus egg (diameter 1.25 mm) new cell membrane is formed in the furrow region (rate of growth approx 4-10(4) mum2/min). Freeze-fracture electron microscopy has produced the following data. Preexisting plasma membrane faces show a reversed polarity with respect to particle distribution, i.e. more particles are attached to the E-face (density 1600-2200 particles/mum2) than to the P-face (300 particles/mum2). A frequency histogram of 2331 measured intramembranous particles does not show a continuous range of sizes. The following sizes were very obvious: 95 A (12%), 125 A (30%) and 180 A (6%). At the tips of surface protrusions both the E- and the P- face are particle-free. Nascent cell membrane fracture faces are more difficult to obtain. The particle density is low (E-face 300-500 particles/mum2). Lowering the ambient temperature to 5 degrees C for approx. 5 mins does not change the normal particle pattern, but it improves the output in nascent membrane fracture faces. The fact that in the Xenopus egg preexisting and nascent membrane regions are continuous but nevertheless maintain their highly different particle densities is noteworthy. The freeze-fracture data are discussed in relation to, among other things, the known values of the specific resistances of these membrane regions.", "contents": "Freeze-fracture electron microscopy of preexisting and nascent cell membrane in cleaving eggs of Xenopus laevis. During cell division in the Xenopus egg (diameter 1.25 mm) new cell membrane is formed in the furrow region (rate of growth approx 4-10(4) mum2/min). Freeze-fracture electron microscopy has produced the following data. Preexisting plasma membrane faces show a reversed polarity with respect to particle distribution, i.e. more particles are attached to the E-face (density 1600-2200 particles/mum2) than to the P-face (300 particles/mum2). A frequency histogram of 2331 measured intramembranous particles does not show a continuous range of sizes. The following sizes were very obvious: 95 A (12%), 125 A (30%) and 180 A (6%). At the tips of surface protrusions both the E- and the P- face are particle-free. Nascent cell membrane fracture faces are more difficult to obtain. The particle density is low (E-face 300-500 particles/mum2). Lowering the ambient temperature to 5 degrees C for approx. 5 mins does not change the normal particle pattern, but it improves the output in nascent membrane fracture faces. The fact that in the Xenopus egg preexisting and nascent membrane regions are continuous but nevertheless maintain their highly different particle densities is noteworthy. The freeze-fracture data are discussed in relation to, among other things, the known values of the specific resistances of these membrane regions.", "PMID": 953012}
{"id": "pubmed23n0032_399", "title": "Transport and metabolism of adenosine in human blood platelets.", "content": "The uptake and metabolism of [14C]- or E[3H] adenosine have been studied in suspensions of washed platelets and in platelet rich plasma. The appearance of radioactivity in the platelets and the formation of radioactive adenosine metabolites have been used to determine the uptake. Adenosine is transported into human blood platelets by two different systems: a low Km system (9.8 muM) which is competitively inhibited by papaverine, and a high Km system (9.4 mM) which is competitively inhibited by adenine. Adenosine transported via the low Km system is probably directly incorporated into adenine nucleotides, while adenosine transported through the high Km system arrives unchanged inside the platelet and is then converted into inosine and hypoxanthine or incorporated into adenine nucleotides.", "contents": "Transport and metabolism of adenosine in human blood platelets. The uptake and metabolism of [14C]- or E[3H] adenosine have been studied in suspensions of washed platelets and in platelet rich plasma. The appearance of radioactivity in the platelets and the formation of radioactive adenosine metabolites have been used to determine the uptake. Adenosine is transported into human blood platelets by two different systems: a low Km system (9.8 muM) which is competitively inhibited by papaverine, and a high Km system (9.4 mM) which is competitively inhibited by adenine. Adenosine transported via the low Km system is probably directly incorporated into adenine nucleotides, while adenosine transported through the high Km system arrives unchanged inside the platelet and is then converted into inosine and hypoxanthine or incorporated into adenine nucleotides.", "PMID": 953013}
{"id": "pubmed23n0032_400", "title": "In vivo modification of plant membrane phospholipid composition.", "content": "Tomato seedlings treated with ethanolamine showed altered phospholipid composition. The changes included altered acyl chain composition as well as changes in the relative amounts of the phospholipid classes. Specifically, there was an increase in phosphatidylethanolamine and phosphatidylserine with a concomitant decrease in phosphatidylcholine and no overall increase in phospholipids. Treatment with ethanolamine increased the relative amount of C18 acyl chains (especially 18 : 2) in phosphatidylethanolamine and phosphatidylcholine at the expense of 16 : 0 and 16 : 1. Acyl composition of other phospholipid classes were unchanged. Labeled ethanolamine was incorporated mostly into phosphatidylethanolamine and phosphatidylcholine. Ethanolamine-stimulated incorporation of labeled oleate was entirely into acyl chains and appeared only as 18 : 1 and 18 : 2. There was greater incorporation, but less conversion of 18 : 1 to 18 : 2 with choline. Stearate was incorporated but desaturated.", "contents": "In vivo modification of plant membrane phospholipid composition. Tomato seedlings treated with ethanolamine showed altered phospholipid composition. The changes included altered acyl chain composition as well as changes in the relative amounts of the phospholipid classes. Specifically, there was an increase in phosphatidylethanolamine and phosphatidylserine with a concomitant decrease in phosphatidylcholine and no overall increase in phospholipids. Treatment with ethanolamine increased the relative amount of C18 acyl chains (especially 18 : 2) in phosphatidylethanolamine and phosphatidylcholine at the expense of 16 : 0 and 16 : 1. Acyl composition of other phospholipid classes were unchanged. Labeled ethanolamine was incorporated mostly into phosphatidylethanolamine and phosphatidylcholine. Ethanolamine-stimulated incorporation of labeled oleate was entirely into acyl chains and appeared only as 18 : 1 and 18 : 2. There was greater incorporation, but less conversion of 18 : 1 to 18 : 2 with choline. Stearate was incorporated but desaturated.", "PMID": 953014}
{"id": "pubmed23n0032_401", "title": "Ionic permeability of epithelial tissues.", "content": "The overall permeability of epithelial tissues to solutes is generally determined by analyzing net or unidirectional transepithelial fluxes in response to transepithelial differences of concentration and/or electrical potential using relations that describe diffusional movements across a single membrane. If the solute is uncharged and diffusional movements are transcellular, the overall transepithelial permeability coefficient is determined by the permeabilities of the two limiting cell membranes combinded in series. However, if the solute is charged and the pathway for transepithelial movement involves diffusional flows across at least two membranes arranged in series (i.e. transcellular transport), the value of the overall transepithelial permeability coefficient determined using relations that describe ionic diffusion across a single membrane is not an accurate measure of the permeabilities of the two limiting membranes combined in series. Further, if ionic diffusion is transcellular, permeability coefficients determined from studies of transepithelial fluxes are not only quantitatively incorrect but can also result in grossly erroneous interpretations of changes in transepithelial permeabilities and faulty inferences regarding the route of transepithelial ionic diffusion.", "contents": "Ionic permeability of epithelial tissues. The overall permeability of epithelial tissues to solutes is generally determined by analyzing net or unidirectional transepithelial fluxes in response to transepithelial differences of concentration and/or electrical potential using relations that describe diffusional movements across a single membrane. If the solute is uncharged and diffusional movements are transcellular, the overall transepithelial permeability coefficient is determined by the permeabilities of the two limiting cell membranes combinded in series. However, if the solute is charged and the pathway for transepithelial movement involves diffusional flows across at least two membranes arranged in series (i.e. transcellular transport), the value of the overall transepithelial permeability coefficient determined using relations that describe ionic diffusion across a single membrane is not an accurate measure of the permeabilities of the two limiting membranes combined in series. Further, if ionic diffusion is transcellular, permeability coefficients determined from studies of transepithelial fluxes are not only quantitatively incorrect but can also result in grossly erroneous interpretations of changes in transepithelial permeabilities and faulty inferences regarding the route of transepithelial ionic diffusion.", "PMID": 953015}
{"id": "pubmed23n0032_402", "title": "The effect of temperature on the galactosyl- and sialyltransferases and on the ultrastructure of Golgi membranes.", "content": "The effect of temperature on the activity of galactosyl- and sialyltransferases of rat liver Golgi membranes and the galactosyltransferase of serum has been studied. Arrhenius plots for three enzymes were different. Sharp breaks in the curves, indicative of phase transitions were observed for sialyltransferase (28 degree C) of Golgi and galactosyltransferase (34 degree C) of serum but not for galactosyltransferase of Golgi. The activation energy was greater above the break (above 28 degree C) than below for sialyltransferase of Golgi; The activation energy was lower (above 34 degree C) for galactosyltransferase of serum than below. Electron microscopic freeze replicas showed a patchy distribution of particles which increased as the temperature was raised accompanied by smooth areas. This was interpreted as representing lateral phase separation of the membrane components.", "contents": "The effect of temperature on the galactosyl- and sialyltransferases and on the ultrastructure of Golgi membranes. The effect of temperature on the activity of galactosyl- and sialyltransferases of rat liver Golgi membranes and the galactosyltransferase of serum has been studied. Arrhenius plots for three enzymes were different. Sharp breaks in the curves, indicative of phase transitions were observed for sialyltransferase (28 degree C) of Golgi and galactosyltransferase (34 degree C) of serum but not for galactosyltransferase of Golgi. The activation energy was greater above the break (above 28 degree C) than below for sialyltransferase of Golgi; The activation energy was lower (above 34 degree C) for galactosyltransferase of serum than below. Electron microscopic freeze replicas showed a patchy distribution of particles which increased as the temperature was raised accompanied by smooth areas. This was interpreted as representing lateral phase separation of the membrane components.", "PMID": 953016}
{"id": "pubmed23n0032_403", "title": "Transport of 2-deoxy-D-galactose in Saccharomyces fragilis.", "content": "2-Deoxy-D-galactose (dGal) transport in Saccharomyces fragilis is characterized by energy requirement and accumulation of the free sugar against a concentration gradient, indicating active transport. Besides free sugar dGal-1-phosphate, UDP-dGal and a trehalose-like derivative were found inside the cells. The accumulation of the phosphorylated derivatives was balanced by a concomitant decrease of ATP, orthophosphate and polyphosphates. With pulse labeling experiments it could be shown that the free sugar is transported into the cells. This conclusion was supported by several other experimental results, e.g. the lack of correlation between the sugar transport parameters and the dGal phosphorylation capacity, and the countertransport of free dGal evoked by galactose in the medium. The typical differences between this active transport mechanism and the transport-associated phosphorylation system, described previously, are discussed.", "contents": "Transport of 2-deoxy-D-galactose in Saccharomyces fragilis. 2-Deoxy-D-galactose (dGal) transport in Saccharomyces fragilis is characterized by energy requirement and accumulation of the free sugar against a concentration gradient, indicating active transport. Besides free sugar dGal-1-phosphate, UDP-dGal and a trehalose-like derivative were found inside the cells. The accumulation of the phosphorylated derivatives was balanced by a concomitant decrease of ATP, orthophosphate and polyphosphates. With pulse labeling experiments it could be shown that the free sugar is transported into the cells. This conclusion was supported by several other experimental results, e.g. the lack of correlation between the sugar transport parameters and the dGal phosphorylation capacity, and the countertransport of free dGal evoked by galactose in the medium. The typical differences between this active transport mechanism and the transport-associated phosphorylation system, described previously, are discussed.", "PMID": 953017}
{"id": "pubmed23n0032_404", "title": "D-glucose uptake by a rat liver plasma membrane preparation.", "content": "1. Plasma membranes isolated from rat liver according to a modification of the method of Neville, D.M. ((1960) J. Biophys. Biochem. Cytol. 8, 413-422) were used as model to test current hypotheses on the mode of glucose uptake into the liver cell. Glucose uptake studies were performed by a filtration technique using labeled glucose analogues. 2. D-glucose Uptake by rat liver plasma membranes is characterized by features of simple diffusion, i.e. linearity of uptake, lack of stereospecificity, and by facilitated diffusion, i.e. temperature dependence, counterflow phenomenon and inhibition by phloretin. These findings confirm earlier studies on liver slices and perfused liver. 3. Binding studies on sonicated membranes provide evidence for a specific binding site or protein for D-glucose at the plasma membrane by isolating Tris-soluble membrane proteins which reveal a higher binding capacity than the unsonicated membrane. 4. These findings are interpreted as showing the presence of a \"carrier\" mediated transport system for D-glucose superimposed by free diffusion due to artificial disruption of the plasma membranes.", "contents": "D-glucose uptake by a rat liver plasma membrane preparation. 1. Plasma membranes isolated from rat liver according to a modification of the method of Neville, D.M. ((1960) J. Biophys. Biochem. Cytol. 8, 413-422) were used as model to test current hypotheses on the mode of glucose uptake into the liver cell. Glucose uptake studies were performed by a filtration technique using labeled glucose analogues. 2. D-glucose Uptake by rat liver plasma membranes is characterized by features of simple diffusion, i.e. linearity of uptake, lack of stereospecificity, and by facilitated diffusion, i.e. temperature dependence, counterflow phenomenon and inhibition by phloretin. These findings confirm earlier studies on liver slices and perfused liver. 3. Binding studies on sonicated membranes provide evidence for a specific binding site or protein for D-glucose at the plasma membrane by isolating Tris-soluble membrane proteins which reveal a higher binding capacity than the unsonicated membrane. 4. These findings are interpreted as showing the presence of a \"carrier\" mediated transport system for D-glucose superimposed by free diffusion due to artificial disruption of the plasma membranes.", "PMID": 953018}
{"id": "pubmed23n0032_405", "title": "Studies on the mechanism of phosphorylation and transport of beta-galactosides by the lactose phosphotransferase system of Staphylococcus aureus. Kinetic investigations using tosyl galactosides as reversible dead-end inhibitors.", "content": "Tosyl galactosides, previously shown to be potent reversible dead-end inhibitors of the membrane-bound Enzyme IIlac of the lactose phosphotransferase system of Staphylococcus aureus, were used for an investigation of the kinetic mechanism of the sugar phosphorylation/transport reaction catalyzed by this enzyme: phospho-Factor IIIlac&sugar Enzyme IIlac lead to Factor IIIlac&sugar phosphate. Inhibition of Enzyme IIlac was studied in three different systems. Washed membranes, and washed membranes in the presence of 0.1% Triton X-100 were used for phosphorylation experiments, and whole cells were used for transport studies. When washed membranes were used to supply Enzyme IIlac, inhibition of phosphorylation by tosyl galactoside was linear non-competitive against both the sugar and phospho-Factor IIIlac substrates, with an apparent Ki of about 0.5 mM. This Ki decreased with increasing Factor IIIlac concentration. In the presence of 0.1% Triton X-100, the phosphorylation reaction was stimulated; under these conditions the inhibition became strictly competitive against sugar, and completely uncompetitive against phospho-Factor IIIlac. Apparently washed membranes can catalyze phosphorylation both via a reaction sequence in which sugar binds first and via one in which phospho-Factor IIIlac binds first, but in the presence of 0.1% Triton the reaction does not occur by the former sequence. The inability of bound phospho-Factor IIIlac to hinder the binding of tosyl galactosides suggests that the initial binding sites of the two substrates of Enzyme IIlac are separated by at least the distance of the tosyl moiety. Radioactive methyl 6-O-(p-toluenesulfonyl) beta-galactoside was not converted into a phosphorylated product in the reaction mixtures, i.e. it is a true dead-end inhibitor. Inhibition of beta-galactoside transport into whole cells by tosyl galactosides was competitive, with an apparent Ki of 5-10 mM, an order of magnitude higher than the Ki for inhibition of phosphorylation by membrane preparations. This result suggest that a significant level of unphosphorylated phospho-Factor IIIlac is present inside the cells, or that cellular levels of this compound are considerably lower than those used for in vitro sugar phosphorylation assays. Radioactive tosyl galactoside inhibitor was not transported into whole cells.", "contents": "Studies on the mechanism of phosphorylation and transport of beta-galactosides by the lactose phosphotransferase system of Staphylococcus aureus. Kinetic investigations using tosyl galactosides as reversible dead-end inhibitors. Tosyl galactosides, previously shown to be potent reversible dead-end inhibitors of the membrane-bound Enzyme IIlac of the lactose phosphotransferase system of Staphylococcus aureus, were used for an investigation of the kinetic mechanism of the sugar phosphorylation/transport reaction catalyzed by this enzyme: phospho-Factor IIIlac&sugar Enzyme IIlac lead to Factor IIIlac&sugar phosphate. Inhibition of Enzyme IIlac was studied in three different systems. Washed membranes, and washed membranes in the presence of 0.1% Triton X-100 were used for phosphorylation experiments, and whole cells were used for transport studies. When washed membranes were used to supply Enzyme IIlac, inhibition of phosphorylation by tosyl galactoside was linear non-competitive against both the sugar and phospho-Factor IIIlac substrates, with an apparent Ki of about 0.5 mM. This Ki decreased with increasing Factor IIIlac concentration. In the presence of 0.1% Triton X-100, the phosphorylation reaction was stimulated; under these conditions the inhibition became strictly competitive against sugar, and completely uncompetitive against phospho-Factor IIIlac. Apparently washed membranes can catalyze phosphorylation both via a reaction sequence in which sugar binds first and via one in which phospho-Factor IIIlac binds first, but in the presence of 0.1% Triton the reaction does not occur by the former sequence. The inability of bound phospho-Factor IIIlac to hinder the binding of tosyl galactosides suggests that the initial binding sites of the two substrates of Enzyme IIlac are separated by at least the distance of the tosyl moiety. Radioactive methyl 6-O-(p-toluenesulfonyl) beta-galactoside was not converted into a phosphorylated product in the reaction mixtures, i.e. it is a true dead-end inhibitor. Inhibition of beta-galactoside transport into whole cells by tosyl galactosides was competitive, with an apparent Ki of 5-10 mM, an order of magnitude higher than the Ki for inhibition of phosphorylation by membrane preparations. This result suggest that a significant level of unphosphorylated phospho-Factor IIIlac is present inside the cells, or that cellular levels of this compound are considerably lower than those used for in vitro sugar phosphorylation assays. Radioactive tosyl galactoside inhibitor was not transported into whole cells.", "PMID": 953019}
{"id": "pubmed23n0032_406", "title": "Computer-simulated urea reflection coefficients in human red cells.", "content": "Relative volume curves for human red cells were generated with a computer program to simulate the experimental results obtained by rapidly mixing red cells and urea. Known values for the permeability variables were used in the Kedem and Katchalsky (Biochim. Biophys. Acta (1958) 27, 229-246) solute and solvent flux equations. The different values used for the reflection coefficient, o, were 0.62, 0.75 and 1.0. The theoretical computer curves with o = 0.75 compared closely with the experimental stopped-flow curves.", "contents": "Computer-simulated urea reflection coefficients in human red cells. Relative volume curves for human red cells were generated with a computer program to simulate the experimental results obtained by rapidly mixing red cells and urea. Known values for the permeability variables were used in the Kedem and Katchalsky (Biochim. Biophys. Acta (1958) 27, 229-246) solute and solvent flux equations. The different values used for the reflection coefficient, o, were 0.62, 0.75 and 1.0. The theoretical computer curves with o = 0.75 compared closely with the experimental stopped-flow curves.", "PMID": 953020}
{"id": "pubmed23n0032_407", "title": "Extracellular ascorbic acid in lung.", "content": "Fifty percent of the ascorbic acid content of sliced rat lung was released from the tissue to the media within a few minutes by either washing or incubating the slices with Krebs-phosphate solution. Measurement of the lactate dehydrogenase and potassium content of the medium after incubating lung slices for 5 min showed that about 20% of the cells were damaged by slicing. Sephadex chromatography of tissue extracts prepared from washed lung slices showed that none of the ascorbic acid in these slices were bound to protein. Also, metabolic poisons were shown to deplete the ascorbic acid content of washed lung slices. Approx. 57% of the lung ascorbic acid of guinea pigs that had been supplemented with ascorbic acid and 78% of the lung ascorbic acid of ascorbic acid-deficient guinea pigs were found in the medium when lung slices from these animals were incubated with Krebs-phosphate solution. These results were taken to indicate the presence of an extracellular pool of ascorbic acid in lung which is maintained even during scurvy.", "contents": "Extracellular ascorbic acid in lung. Fifty percent of the ascorbic acid content of sliced rat lung was released from the tissue to the media within a few minutes by either washing or incubating the slices with Krebs-phosphate solution. Measurement of the lactate dehydrogenase and potassium content of the medium after incubating lung slices for 5 min showed that about 20% of the cells were damaged by slicing. Sephadex chromatography of tissue extracts prepared from washed lung slices showed that none of the ascorbic acid in these slices were bound to protein. Also, metabolic poisons were shown to deplete the ascorbic acid content of washed lung slices. Approx. 57% of the lung ascorbic acid of guinea pigs that had been supplemented with ascorbic acid and 78% of the lung ascorbic acid of ascorbic acid-deficient guinea pigs were found in the medium when lung slices from these animals were incubated with Krebs-phosphate solution. These results were taken to indicate the presence of an extracellular pool of ascorbic acid in lung which is maintained even during scurvy.", "PMID": 953021}
{"id": "pubmed23n0032_408", "title": "Nitrogenase activity and respiration of cultures of Rhizobium spp. with special reference to concentrations of dissolved oxygen.", "content": "Studies of nitrogenase in cultures of the cowpea rhizobia (Rhizobium spp.) strains 32H1 and CB756 are reported. Preliminary experiments established that, even when agar cultures were grown in air, suspensions of bacteria prepared anaerobically from them were most active at low concentrations of free dissolved O2. Consequently, assays for activity used low concentrations of O2, stabilized by adding the nodule pigment leghaemoglobin. In continuous, glutamine-limited cultures of 32H1, nitrogenase activity appeared only when the concentration of dissolved O2 in the cultures approached 1 muM. Lowering the glutamine concentration in the medium supplied to the culture from 2 to 1 mM halved the cell yield and nitrogenase activity was also diminished. Omitting succinate from the medium caused the concentration of dissolved O2 to rise and nitrogenase activity was lost. Upon restoration of the succinate supply, the O2 concentration immediately fell and nitrogenase was restored. The activity doubled in about 8 h, whereas the doubling time of this culture was 14 h. Sonic extracts of 32H1 cells from continuous cultures with active nitrogenase contained components reacting with antiserum against nitrogenase Mo-Fe protein from soybean bacteroids. Continuous cultures grown at higher O2 concentration, with only a trace of active nitrogenase, contained less of these antigens and they were not detected in highly aerobic cultures. Nitrogenase activity of a continuous culture was repressed by NH+4; the apparent half-life was about 90 min. Cells of 32H1 from a continuous culture growing at between 30 and 100 muM dissolved O2 possessed a protective mechanism which permitted respiration to increase following exposure to a rapid increase in O2 concentration from low levels (O2 shock). This effect disappeared as the O2 concentration for growth was reduced towards 1 muM.", "contents": "Nitrogenase activity and respiration of cultures of Rhizobium spp. with special reference to concentrations of dissolved oxygen. Studies of nitrogenase in cultures of the cowpea rhizobia (Rhizobium spp.) strains 32H1 and CB756 are reported. Preliminary experiments established that, even when agar cultures were grown in air, suspensions of bacteria prepared anaerobically from them were most active at low concentrations of free dissolved O2. Consequently, assays for activity used low concentrations of O2, stabilized by adding the nodule pigment leghaemoglobin. In continuous, glutamine-limited cultures of 32H1, nitrogenase activity appeared only when the concentration of dissolved O2 in the cultures approached 1 muM. Lowering the glutamine concentration in the medium supplied to the culture from 2 to 1 mM halved the cell yield and nitrogenase activity was also diminished. Omitting succinate from the medium caused the concentration of dissolved O2 to rise and nitrogenase activity was lost. Upon restoration of the succinate supply, the O2 concentration immediately fell and nitrogenase was restored. The activity doubled in about 8 h, whereas the doubling time of this culture was 14 h. Sonic extracts of 32H1 cells from continuous cultures with active nitrogenase contained components reacting with antiserum against nitrogenase Mo-Fe protein from soybean bacteroids. Continuous cultures grown at higher O2 concentration, with only a trace of active nitrogenase, contained less of these antigens and they were not detected in highly aerobic cultures. Nitrogenase activity of a continuous culture was repressed by NH+4; the apparent half-life was about 90 min. Cells of 32H1 from a continuous culture growing at between 30 and 100 muM dissolved O2 possessed a protective mechanism which permitted respiration to increase following exposure to a rapid increase in O2 concentration from low levels (O2 shock). This effect disappeared as the O2 concentration for growth was reduced towards 1 muM.", "PMID": 953022}
{"id": "pubmed23n0032_409", "title": "Wheat germ agglutinin. Evidence for a genetic basis of multiple forms.", "content": "Germ from hexaploid wheat (Triticum aestivum L.) contained three forms of agglutinin separable by ion-exchange column chromatography at pH 3.8, while germ from tetraploid wheat (Triticum turgidum L. (durum group)) contained only two such forms. The different number of forms, not due to protein modification occurring during the purification process, was demonstrable in commercial germ and in bran fractions containing germ from single wheat varieties. This evidence for a genetic basis of lectin multiple forms in wheat indicates the advisability of using genetically identified plant varieties in lectin research.", "contents": "Wheat germ agglutinin. Evidence for a genetic basis of multiple forms. Germ from hexaploid wheat (Triticum aestivum L.) contained three forms of agglutinin separable by ion-exchange column chromatography at pH 3.8, while germ from tetraploid wheat (Triticum turgidum L. (durum group)) contained only two such forms. The different number of forms, not due to protein modification occurring during the purification process, was demonstrable in commercial germ and in bran fractions containing germ from single wheat varieties. This evidence for a genetic basis of lectin multiple forms in wheat indicates the advisability of using genetically identified plant varieties in lectin research.", "PMID": 953023}
{"id": "pubmed23n0032_410", "title": "The mycelial cell wall of Penicillium charlesii G. Smith NRRL 1887.", "content": "Mycelial cell wall preparations of Penicillium charlesii G. Smith have been shown to have a total neutral carbohydrate content of 69%. Glucosamine which comprises 12% of the wall is present principally as chitin. Lipids comprise 37.5% of the wall while polypeptides comprise 19.5%. No galactofuranose units are present in the wall of this organism which is known to produce an extracellular beta-1,5-galactofuranose containing polymer.", "contents": "The mycelial cell wall of Penicillium charlesii G. Smith NRRL 1887. Mycelial cell wall preparations of Penicillium charlesii G. Smith have been shown to have a total neutral carbohydrate content of 69%. Glucosamine which comprises 12% of the wall is present principally as chitin. Lipids comprise 37.5% of the wall while polypeptides comprise 19.5%. No galactofuranose units are present in the wall of this organism which is known to produce an extracellular beta-1,5-galactofuranose containing polymer.", "PMID": 953024}
{"id": "pubmed23n0032_411", "title": "Quantitative microanalysis of cell walls of Saprolegnia diclina Humphrey and Tremella mesenterica Fries.", "content": "Cell walls of the fungi Saprolegnia declina Humphrey and Tremella mesenterica Fries were analyzed quantitatively. Particular attention was paid to the hydrolysis and analysis of neutral sugars, amino sugars and amino acids. These components, together with total lipids, total uronic acids and the ashed residue, accounted for more than 90% by weight of the original dry cell wall preparation. There were substantial losses of amino acids during hydrolysis; however, analytical recovery approached 100% when total protein was calculated from the total nitrogen analysis. The analytical procedures were reproducible (+/- 3% for amino acids and amino sugars, and +/- 5-10% for other components) when applied to individual cell wall preparations. However, even under carefully standardized conditions, different cell wall preparations from the same species showed variable composition. Glucose was the predominant neutral sugar in the cell wall polymers of both species. The amino acid compositions were remarkable in that neither species contained detectable levels of cyst(e)ine. Hydroxyproline was detected in both species. The report from Tremella mesenterica is the first for this amino acid from the cell wall of a Basidiomycete.", "contents": "Quantitative microanalysis of cell walls of Saprolegnia diclina Humphrey and Tremella mesenterica Fries. Cell walls of the fungi Saprolegnia declina Humphrey and Tremella mesenterica Fries were analyzed quantitatively. Particular attention was paid to the hydrolysis and analysis of neutral sugars, amino sugars and amino acids. These components, together with total lipids, total uronic acids and the ashed residue, accounted for more than 90% by weight of the original dry cell wall preparation. There were substantial losses of amino acids during hydrolysis; however, analytical recovery approached 100% when total protein was calculated from the total nitrogen analysis. The analytical procedures were reproducible (+/- 3% for amino acids and amino sugars, and +/- 5-10% for other components) when applied to individual cell wall preparations. However, even under carefully standardized conditions, different cell wall preparations from the same species showed variable composition. Glucose was the predominant neutral sugar in the cell wall polymers of both species. The amino acid compositions were remarkable in that neither species contained detectable levels of cyst(e)ine. Hydroxyproline was detected in both species. The report from Tremella mesenterica is the first for this amino acid from the cell wall of a Basidiomycete.", "PMID": 953025}
{"id": "pubmed23n0032_412", "title": "The dissociation of insulin from human insulin antibodies.", "content": "The dissociation of insulin from human insulin antibodies has been investigated using a technique that is rapid and does not require addition of excess unlabelled insulin. A slow (k1 = 2-10(-3) min-1) and a fast k2 = 4-10(-2) min-1) dissociating antibody component were identified in all studies. These have been shown to correspond, respectively, to the high and low affinity antibody components of equilibrium binding studies. The range of k1 and k2 values and their response to temperature change is small. Insulin resistance and stability of diabetes are not related to properties of antibody dissociation. Dissociation is faster in the presence of high (6-850 nM) insulin concentration due to increased binding to the fast dissociating component without change in the dissociation rate constants. When incubation time is increased beyond achievement of maximal binding there is a time-depent rise in binding to the slow dissociating component, with a concomitant fall in k1. The traditional concept that equilibrium is established at maximum binding requires further examination.", "contents": "The dissociation of insulin from human insulin antibodies. The dissociation of insulin from human insulin antibodies has been investigated using a technique that is rapid and does not require addition of excess unlabelled insulin. A slow (k1 = 2-10(-3) min-1) and a fast k2 = 4-10(-2) min-1) dissociating antibody component were identified in all studies. These have been shown to correspond, respectively, to the high and low affinity antibody components of equilibrium binding studies. The range of k1 and k2 values and their response to temperature change is small. Insulin resistance and stability of diabetes are not related to properties of antibody dissociation. Dissociation is faster in the presence of high (6-850 nM) insulin concentration due to increased binding to the fast dissociating component without change in the dissociation rate constants. When incubation time is increased beyond achievement of maximal binding there is a time-depent rise in binding to the slow dissociating component, with a concomitant fall in k1. The traditional concept that equilibrium is established at maximum binding requires further examination.", "PMID": 953026}
{"id": "pubmed23n0032_413", "title": "Glucocorticoid binding in the chicken liver cytosol. Characterization of five macromolecular binding components.", "content": "The heterogeneity of the glucocorticoid binding in the chicken liver cytosol, previously suggested by the results obtained with crude preparations, was confirmed using different techniques such as stepwise ammonium sulfate precipitation, hydroxyapatite chromatography and gel filtration on Sephadex G-200. The latter method permitted the separation of five glucocorticoid binding macromolecules respectively named binders S1, S2, S3, S4 and S5, according to their decreasing apparent molecular size upon gel filtration. Apparent molecular weight, binding affinity, capacity and specificity of these five moieties were examined. In addition, S-aryl-transferase activity using glutathione as co-substrate was studied and found to coincide mostly with the fractions containing binder S4, which might represent an avian liver ligandin.", "contents": "Glucocorticoid binding in the chicken liver cytosol. Characterization of five macromolecular binding components. The heterogeneity of the glucocorticoid binding in the chicken liver cytosol, previously suggested by the results obtained with crude preparations, was confirmed using different techniques such as stepwise ammonium sulfate precipitation, hydroxyapatite chromatography and gel filtration on Sephadex G-200. The latter method permitted the separation of five glucocorticoid binding macromolecules respectively named binders S1, S2, S3, S4 and S5, according to their decreasing apparent molecular size upon gel filtration. Apparent molecular weight, binding affinity, capacity and specificity of these five moieties were examined. In addition, S-aryl-transferase activity using glutathione as co-substrate was studied and found to coincide mostly with the fractions containing binder S4, which might represent an avian liver ligandin.", "PMID": 953027}
{"id": "pubmed23n0032_414", "title": "Regulation of the pyruvate dehydrogenase activity in the isolated perfused heart of guinea-pigs.", "content": "The activity and the interconversion of the between the pyruvate after pyruvate should read: utilization in the perfused hearts and the pyruvate dehydrogenease complex has been measured in the isolated perfused working hearts of guinea-pigs. 1. The pyruvate dehydrogenase complex is transferred into the active form by high work, in anoxia, with 2,4-dinitrophenol and by perfusion without substrate. The rate of interconversion is faster in the perfused heart than in the homogenate. 2. The active form of the pyruvate dehydrogenase complex limits the pyruvate oxidation. There is a close correlation between the pyruvate utilization in the perfused hearts and the pyruvate dehydrogenase of the active form in the homogenates of the same hearts. 3. The \"adenylate energy charge\" of the cells is considered as the main regulating factor of the interconversion of the pyruvate dehydrogenase complex as seen in experiments with anoxia, dinitrophenol and high work. The inactivation of the pyruvate dehydrogenase complex by acetyl CoA can be overcome by decreasing ATP/ADP ratios.", "contents": "Regulation of the pyruvate dehydrogenase activity in the isolated perfused heart of guinea-pigs. The activity and the interconversion of the between the pyruvate after pyruvate should read: utilization in the perfused hearts and the pyruvate dehydrogenease complex has been measured in the isolated perfused working hearts of guinea-pigs. 1. The pyruvate dehydrogenase complex is transferred into the active form by high work, in anoxia, with 2,4-dinitrophenol and by perfusion without substrate. The rate of interconversion is faster in the perfused heart than in the homogenate. 2. The active form of the pyruvate dehydrogenase complex limits the pyruvate oxidation. There is a close correlation between the pyruvate utilization in the perfused hearts and the pyruvate dehydrogenase of the active form in the homogenates of the same hearts. 3. The \"adenylate energy charge\" of the cells is considered as the main regulating factor of the interconversion of the pyruvate dehydrogenase complex as seen in experiments with anoxia, dinitrophenol and high work. The inactivation of the pyruvate dehydrogenase complex by acetyl CoA can be overcome by decreasing ATP/ADP ratios.", "PMID": 953028}
{"id": "pubmed23n0032_415", "title": "Formation of mannosyl-lipids by an ectomannosyltransferase in suspensions of BALB/c fibroblasts.", "content": "A mannosyltransferase has been detected in suspensions of BALB/c fibroblasts incubated with GDP-[14C] mannose. The experimental evidence indicates the cell surface as the most likely site for the enzyme. The transferase synthesizes both glycolipids and glycoproteins. The lipid compounds have properties suggestive of lipid-linked mono- and oligosaccharides which can function as intermediates in glycoprotein synthesis. The formation of these compounds by a cell surface enzyme suggests that lipid-linked intermediates may play an important role in the glycosylation of membrane components.", "contents": "Formation of mannosyl-lipids by an ectomannosyltransferase in suspensions of BALB/c fibroblasts. A mannosyltransferase has been detected in suspensions of BALB/c fibroblasts incubated with GDP-[14C] mannose. The experimental evidence indicates the cell surface as the most likely site for the enzyme. The transferase synthesizes both glycolipids and glycoproteins. The lipid compounds have properties suggestive of lipid-linked mono- and oligosaccharides which can function as intermediates in glycoprotein synthesis. The formation of these compounds by a cell surface enzyme suggests that lipid-linked intermediates may play an important role in the glycosylation of membrane components.", "PMID": 953029}
{"id": "pubmed23n0032_416", "title": "Acetylcholinesterase from the house-fly head. Molecular properties of soluble forms.", "content": "1. Polyacrylamide gel electrophoresis in Tris/glycine buffer (pH 8.3) revealed five forms of acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) in the 100 000 X g, 1-h supernatants of aqueous fly-head extracts from the DDT/S strain. Five other housefly strains (CSMA, Bayer 21/199, Cradson/P, Malathion/R and DDT/R)were shown qualitatively to have the same soluble forms of the enzyme. 2. Plots of the electrophoretic mobility versus polyacrylamide concentration indicated that the multiple forms constituted a size isomer family. From the retardation coefficients derived from these plots, molecular weight estimates were obtained; these suggested that the smallest active component was a form of approx. 80 000 daltons. The higher aggregates, however, did not appear as simple oligomers of this component. 3. Density gradient sedimentation supported the electrophoretic findings. The smallest active component, with a sedimentation coefficient of 5.3 S, was confirmed as a molecular species of acetylcholinesterase that has not previously been obtained from house-flies; higher aggregates gave sedimentation coefficients of 7.4, 7.8. 8.1, and 11.8 S. 4. Gel-filtration on calibrated Sephadex G-150 columns provided further evidence that the smallest active component was a form of about 80 000 daltons. 5. Autolysis converted much of the particulate enzyme and all of the soluble forms into a species of approx. 160 000 daltons indistinguishable from the native 7.4-S form. Both the autolysed enzyme and the native 7.4-S form were susceptible to cleavage by disulphide reducing agents, and released catalytically active subunits that corresponded to the 5.3-S form of 80 000 daltons. The data were compatible with a monomer-dimer relationship between the 5.3-S and 7.4-S forms. 6. The possibility is suggested that a form of molecular weight approx. 80 000 constitutes the \"fundamental unit\" of insect cholinesterase.", "contents": "Acetylcholinesterase from the house-fly head. Molecular properties of soluble forms. 1. Polyacrylamide gel electrophoresis in Tris/glycine buffer (pH 8.3) revealed five forms of acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) in the 100 000 X g, 1-h supernatants of aqueous fly-head extracts from the DDT/S strain. Five other housefly strains (CSMA, Bayer 21/199, Cradson/P, Malathion/R and DDT/R)were shown qualitatively to have the same soluble forms of the enzyme. 2. Plots of the electrophoretic mobility versus polyacrylamide concentration indicated that the multiple forms constituted a size isomer family. From the retardation coefficients derived from these plots, molecular weight estimates were obtained; these suggested that the smallest active component was a form of approx. 80 000 daltons. The higher aggregates, however, did not appear as simple oligomers of this component. 3. Density gradient sedimentation supported the electrophoretic findings. The smallest active component, with a sedimentation coefficient of 5.3 S, was confirmed as a molecular species of acetylcholinesterase that has not previously been obtained from house-flies; higher aggregates gave sedimentation coefficients of 7.4, 7.8. 8.1, and 11.8 S. 4. Gel-filtration on calibrated Sephadex G-150 columns provided further evidence that the smallest active component was a form of about 80 000 daltons. 5. Autolysis converted much of the particulate enzyme and all of the soluble forms into a species of approx. 160 000 daltons indistinguishable from the native 7.4-S form. Both the autolysed enzyme and the native 7.4-S form were susceptible to cleavage by disulphide reducing agents, and released catalytically active subunits that corresponded to the 5.3-S form of 80 000 daltons. The data were compatible with a monomer-dimer relationship between the 5.3-S and 7.4-S forms. 6. The possibility is suggested that a form of molecular weight approx. 80 000 constitutes the \"fundamental unit\" of insect cholinesterase.", "PMID": 953030}
{"id": "pubmed23n0032_417", "title": "Acetylcholinesterase of the house-fly head. Affinity purification and subunit composition.", "content": "1. Acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) of house-fly head tissue was solubilized as a 7.4-S form by autolysis for 80-100 h at 25 degrees C and pH 8.0. 2. The autolysed enzyme was purified by affinity chromatography, firstly on Con-A-Sepharose and subsequently on m-trimethylammoniumaniline-Affi-Gel 202. This sequence permitted overall purification yields of approx. 50% of the solubilized enzyme. 3. The 7.4-S purified enzyme was essentially homogeneous on polyacrylamide gel electrophoresis, and its specific activity coincided with the highest previously reported for fly-head acetylcholinesterase. 4. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate and beta-mercaptoethanol revealed two major polypeptide components of molecular weight 82 000 and 59 000. Each of these polypeptides contained diisopropylphosphofluoridate-binding sites, as shown with [3H] diisopropylphosphofluoridate. 5. The results suggest a strong structural similarity between fly-head acetylcholinesterase and the purified electric eel enzyme.", "contents": "Acetylcholinesterase of the house-fly head. Affinity purification and subunit composition. 1. Acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) of house-fly head tissue was solubilized as a 7.4-S form by autolysis for 80-100 h at 25 degrees C and pH 8.0. 2. The autolysed enzyme was purified by affinity chromatography, firstly on Con-A-Sepharose and subsequently on m-trimethylammoniumaniline-Affi-Gel 202. This sequence permitted overall purification yields of approx. 50% of the solubilized enzyme. 3. The 7.4-S purified enzyme was essentially homogeneous on polyacrylamide gel electrophoresis, and its specific activity coincided with the highest previously reported for fly-head acetylcholinesterase. 4. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate and beta-mercaptoethanol revealed two major polypeptide components of molecular weight 82 000 and 59 000. Each of these polypeptides contained diisopropylphosphofluoridate-binding sites, as shown with [3H] diisopropylphosphofluoridate. 5. The results suggest a strong structural similarity between fly-head acetylcholinesterase and the purified electric eel enzyme.", "PMID": 953031}
{"id": "pubmed23n0032_418", "title": "Purification of urokinase by affinity chromatography.", "content": "Commercially available urokinase (EC 3.4.99.26), though highly active, is still contaminated with unrelated proteins and degradation fragments of urokinase. Further purification of a urokinase preparation by chromatography on benzamidine-Sepharose is described. The final preparation consisted of two molecular forms of urokinase with molecular weights of respectively 31 000 and 54 000. The 54 000-dalton urokinase appears to be composed of two protein chains, one of which is the 31 000-dalton urokinase. A monospecific antiserum against urokinase was raised.", "contents": "Purification of urokinase by affinity chromatography. Commercially available urokinase (EC 3.4.99.26), though highly active, is still contaminated with unrelated proteins and degradation fragments of urokinase. Further purification of a urokinase preparation by chromatography on benzamidine-Sepharose is described. The final preparation consisted of two molecular forms of urokinase with molecular weights of respectively 31 000 and 54 000. The 54 000-dalton urokinase appears to be composed of two protein chains, one of which is the 31 000-dalton urokinase. A monospecific antiserum against urokinase was raised.", "PMID": 953032}
{"id": "pubmed23n0032_419", "title": "Preparation and characterization of two isozymes of choline acetyltransferase from squid head ganglia.", "content": "Two isozymes of choline acetyltransferase (Acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6) have been isolated and purified from squid head ganglia. Each isozyme contains multiple isoelectric forms with isoelectric points ranging from pH 5.0 to 6.2. The isozymes differ in their affinities for cellulose phosphate on column chromatography, as well as in their heat stabilities and in their capacities to be activated by salt. Both isozymes are stabilized by sucrose and by sulfhydryl-protecting reagents such as mercaptoethanol and dithiothreitol.", "contents": "Preparation and characterization of two isozymes of choline acetyltransferase from squid head ganglia. Two isozymes of choline acetyltransferase (Acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6) have been isolated and purified from squid head ganglia. Each isozyme contains multiple isoelectric forms with isoelectric points ranging from pH 5.0 to 6.2. The isozymes differ in their affinities for cellulose phosphate on column chromatography, as well as in their heat stabilities and in their capacities to be activated by salt. Both isozymes are stabilized by sucrose and by sulfhydryl-protecting reagents such as mercaptoethanol and dithiothreitol.", "PMID": 953033}
{"id": "pubmed23n0032_420", "title": "A study of phosphoglycerate kinase in human erythrocytes. II. Kinetic properties.", "content": "Kinetic studies on phosphoglycerate kinase (EC 2.7.2.3) were performed in the forward reaction leading from 1,3-diphosphoglycerate to 3-phosphoglycerate. Substrate activation was observed at fixed levels of ADP or Mg2+ and varying concentrations of 1,3-diphosphoglycerate. A biphasic curve was obtained in both linear and double reciprocal plots demonstrating two Km values (Km1 1.9 - 10(-6) and Km2 9.8 - 10(6) M). Michaelis-Menten-type kinetics were observed in both the linear and double reciprocal plots at fixed levels of 1,3-diphosphoglycerate of ADP and varying concentrations of Mg2+. Apparent Michaelis-Menten kinetics were observed in linear plots when conditions of fixed concentrations of 1,3-diphosphoglycerate or Mg2+ were maintained with varying concentrations of ADP. However, the double-reciprocal plots demonstrated biphasic curves with two Km values (Km1 1.7 - 10(-5) and Km2 1.0 - 10(-4)M). Apparent negative cooperativity was observed with respect to 1,3-diphosphoglycerate and ADP. Phosphoglycerate kinase activity was found to be inhibited by AMP and 2,3-diphosphoglycerate. Substrate activation by 1,3-diphosphoglycerate was maintained in the presence of AMP or 2,3-diphosphoglycerate but at a reduced level of enzyme activity. AMP was found to inhibit enzyme activity non-competitively with respect to 1,3-diphosphoglycerate, ADP and Mg2+. 2,3-Diphosphoglycerate inhibits phosphoglycerate kinase activity with respect to 1,3-diphosphoglycerate, ADP and Mg2+. 2,3-Diphosphoglycerate inhibits phosphoglycerate kinase activity non-competitively with respect of 1,3-diphosphoglycerate.", "contents": "A study of phosphoglycerate kinase in human erythrocytes. II. Kinetic properties. Kinetic studies on phosphoglycerate kinase (EC 2.7.2.3) were performed in the forward reaction leading from 1,3-diphosphoglycerate to 3-phosphoglycerate. Substrate activation was observed at fixed levels of ADP or Mg2+ and varying concentrations of 1,3-diphosphoglycerate. A biphasic curve was obtained in both linear and double reciprocal plots demonstrating two Km values (Km1 1.9 - 10(-6) and Km2 9.8 - 10(6) M). Michaelis-Menten-type kinetics were observed in both the linear and double reciprocal plots at fixed levels of 1,3-diphosphoglycerate of ADP and varying concentrations of Mg2+. Apparent Michaelis-Menten kinetics were observed in linear plots when conditions of fixed concentrations of 1,3-diphosphoglycerate or Mg2+ were maintained with varying concentrations of ADP. However, the double-reciprocal plots demonstrated biphasic curves with two Km values (Km1 1.7 - 10(-5) and Km2 1.0 - 10(-4)M). Apparent negative cooperativity was observed with respect to 1,3-diphosphoglycerate and ADP. Phosphoglycerate kinase activity was found to be inhibited by AMP and 2,3-diphosphoglycerate. Substrate activation by 1,3-diphosphoglycerate was maintained in the presence of AMP or 2,3-diphosphoglycerate but at a reduced level of enzyme activity. AMP was found to inhibit enzyme activity non-competitively with respect to 1,3-diphosphoglycerate, ADP and Mg2+. 2,3-Diphosphoglycerate inhibits phosphoglycerate kinase activity with respect to 1,3-diphosphoglycerate, ADP and Mg2+. 2,3-Diphosphoglycerate inhibits phosphoglycerate kinase activity non-competitively with respect of 1,3-diphosphoglycerate.", "PMID": 953034}
{"id": "pubmed23n0032_421", "title": "The oxidation of dithiothreitol by peroxidases and oxygen.", "content": "Horseradish peroxidase (1.11.1.7), lactoperoxidase (1.11.1.7), and the fragment of cytochrome c known as microperoxidase have been shown to catalyze the oxidation of reduced dithiothreitol in an oxygen-consuming reaction. Evidence for horseradish peroxidase intermediates compound III and compound II has been observed, although ferroperoxidase was not identified during the course of the reaction. The stoichiometry has been extablished as 1 : 1 for oxygen consumed to dithiothreitol oxidized. Cysteine and glutathione have also been shown to be substrates for horseradish peroxidase oxidase reaction.", "contents": "The oxidation of dithiothreitol by peroxidases and oxygen. Horseradish peroxidase (1.11.1.7), lactoperoxidase (1.11.1.7), and the fragment of cytochrome c known as microperoxidase have been shown to catalyze the oxidation of reduced dithiothreitol in an oxygen-consuming reaction. Evidence for horseradish peroxidase intermediates compound III and compound II has been observed, although ferroperoxidase was not identified during the course of the reaction. The stoichiometry has been extablished as 1 : 1 for oxygen consumed to dithiothreitol oxidized. Cysteine and glutathione have also been shown to be substrates for horseradish peroxidase oxidase reaction.", "PMID": 953035}
{"id": "pubmed23n0032_422", "title": "Purification and some properties of human erythrocyte hexokinase.", "content": "1. Human erythrocyte hexokinase (ADP:D-hexose 6-phosphotransferase, EC 2.7.1.1) was purified 50 000--100 000-fold with a final specific activity of about 25--50 units/mg protein using gel-filtration, ion-exchange chromatography and affinity chromagraphy. 2. After isoelectrofocusing ofthe preparation one major protein band could be detected besides a minor band. THe isoelectric point of the major protein band was found to be 4.7. 3. After purification the enzyme could be stabilized in a medium containing inorganic phosphate, glucose, glycerol and mercaptoethanol. 4. The molecular weight was determined by gel-filtration and was found to be 132 000+/-8000. 5. The enzyme shows a broad pH optimum ranging from 7.0 to 8.4. 6. The kinetic behavior of the purified enzyme at 37 degrees C was somewhat different from the normal Michaelis-Menten kinetics due to its instability. The affinity constants were 0.048--0.080 mM for glucose and 0.57--1.0 mM for Mg-ATP. 7. The enzyme was specific for Mg- ATP as the nucleotide substrate. Mg-UTP, Mg-ITP,Mg-GTP and Mg-CTP were not converted to corresponding diphosphates. Several hexoses could be phosphorylated by the enzyme. Mannose could be phosphorylated at the same rate as glucose, although the affinity for the enzyme was lower (5m=0.60mM). Much lower rates and lower affinities were found with 2-deoxy-D-glucose (5m=1.0mM), D(+)-glucosamine (5m=4.5 mM) and fructose (5m=10 mM). N-acetyl-D-glucosamine , galactose andsorbose were not phosphorylated at all.", "contents": "Purification and some properties of human erythrocyte hexokinase. 1. Human erythrocyte hexokinase (ADP:D-hexose 6-phosphotransferase, EC 2.7.1.1) was purified 50 000--100 000-fold with a final specific activity of about 25--50 units/mg protein using gel-filtration, ion-exchange chromatography and affinity chromagraphy. 2. After isoelectrofocusing ofthe preparation one major protein band could be detected besides a minor band. THe isoelectric point of the major protein band was found to be 4.7. 3. After purification the enzyme could be stabilized in a medium containing inorganic phosphate, glucose, glycerol and mercaptoethanol. 4. The molecular weight was determined by gel-filtration and was found to be 132 000+/-8000. 5. The enzyme shows a broad pH optimum ranging from 7.0 to 8.4. 6. The kinetic behavior of the purified enzyme at 37 degrees C was somewhat different from the normal Michaelis-Menten kinetics due to its instability. The affinity constants were 0.048--0.080 mM for glucose and 0.57--1.0 mM for Mg-ATP. 7. The enzyme was specific for Mg- ATP as the nucleotide substrate. Mg-UTP, Mg-ITP,Mg-GTP and Mg-CTP were not converted to corresponding diphosphates. Several hexoses could be phosphorylated by the enzyme. Mannose could be phosphorylated at the same rate as glucose, although the affinity for the enzyme was lower (5m=0.60mM). Much lower rates and lower affinities were found with 2-deoxy-D-glucose (5m=1.0mM), D(+)-glucosamine (5m=4.5 mM) and fructose (5m=10 mM). N-acetyl-D-glucosamine , galactose andsorbose were not phosphorylated at all.", "PMID": 953036}
{"id": "pubmed23n0032_423", "title": "Non-identical behaviour of the subunits of rabbit muscule creatine kinase.", "content": "1. The dimeric enzyme creatine kinase (ATP: creatine N-phospotransferase, EC 2.7.3.2) from rabbit muscle was reacted with three separate reagents, each of which specifically modifies one thiol group per subunit. 2. The reactions of the enzyme with these reagents (4-chloro-7-nitrobenzofurazan, 5,5'-dithiobis-(2-nitrobenzoic acid) and iodoacetate) all behave as normal second-order processes. This indicates that the thiol groups on the two subunits of the enzyme react at the same rate as each other in all three cases. 3. The effects of various ligands (Mg2+, ADP and creatine, and combinations of these) on the kinetics of the reactions were studied. In all cases the reactions behave as normal second-order processes. 4. In the presence of the ligand combination Mg2+ plus ADP plus creatine plus nitrate, which has been postulated to form a \"transition state analogue\" complex with the enzyme, the reactions of the thiol group show considerable deviation from second-order kinetics. This indicates that the thiol groups on the two subunits react at different rates from each other. A similar effect is also noted in the presence of the combination ADP plus creatine plus nitrate. 5. The binding of ADP to the enzyme (studied by equilibrium dialysis) is hyperbolic in the absence of other ligands or in the presence of Mg2+ or Mg2+ plus creatine. The dissociation constant is similar in all three cases. 6. In the presence of creatine plus nitrate (with or without Mg2+) the bindings of ADP to the enzyme is tightened considerably and the binding plots indicate the presence of either negative interactions between the subunits or two distinct types of binding sites. 7. Possible causes for the observed non-identical behaviour of the two subunits of the enzyme are discussed.", "contents": "Non-identical behaviour of the subunits of rabbit muscule creatine kinase. 1. The dimeric enzyme creatine kinase (ATP: creatine N-phospotransferase, EC 2.7.3.2) from rabbit muscle was reacted with three separate reagents, each of which specifically modifies one thiol group per subunit. 2. The reactions of the enzyme with these reagents (4-chloro-7-nitrobenzofurazan, 5,5'-dithiobis-(2-nitrobenzoic acid) and iodoacetate) all behave as normal second-order processes. This indicates that the thiol groups on the two subunits of the enzyme react at the same rate as each other in all three cases. 3. The effects of various ligands (Mg2+, ADP and creatine, and combinations of these) on the kinetics of the reactions were studied. In all cases the reactions behave as normal second-order processes. 4. In the presence of the ligand combination Mg2+ plus ADP plus creatine plus nitrate, which has been postulated to form a \"transition state analogue\" complex with the enzyme, the reactions of the thiol group show considerable deviation from second-order kinetics. This indicates that the thiol groups on the two subunits react at different rates from each other. A similar effect is also noted in the presence of the combination ADP plus creatine plus nitrate. 5. The binding of ADP to the enzyme (studied by equilibrium dialysis) is hyperbolic in the absence of other ligands or in the presence of Mg2+ or Mg2+ plus creatine. The dissociation constant is similar in all three cases. 6. In the presence of creatine plus nitrate (with or without Mg2+) the bindings of ADP to the enzyme is tightened considerably and the binding plots indicate the presence of either negative interactions between the subunits or two distinct types of binding sites. 7. Possible causes for the observed non-identical behaviour of the two subunits of the enzyme are discussed.", "PMID": 953037}
{"id": "pubmed23n0032_424", "title": "Modification of chymotrypsin with the specific cyanate reagent N-acetyl-p-cyanato-l-phenylalanine ethyl ester.", "content": "The specific cyanate reagentN-acetyl-p-cyanato-L-phenylalanine ethyl ester (compound 1) was synthesized in an attempt to selectively modify the binding pocket of chymotrypsin (EC 3.4.21.1) while leaving the catalytic residues un touched. The reagent reacts with chymotrypsin to yield chiefly an inactive derivative 3a, with the active site Ser-195 carbamylated and the alpha-amino group, with the reagent 1 yields a modified enzyme(compound 4) with an additional carbamyl group on Ser-195.Neither derivative 3a nor 4reacts with diisopropylfluorophosphate under conditions where chymotrypsinogen is modified, indicating that Ser-195 is altered. Both derivatives 3a and 4 are retained on a 4-phenylbutylamine affinity colomn demonstrating that the substrate binding pocket is intact in both derivatives. The results indicate the potential value of aryl cyanates as protein reagents for the selective modification of nucleophilic sites. However, it is apparent that reaction at unreactive residues in the binding pocket of chymotrypsin with cyanates or similar reagents will require blockage of the more nucleophilic catalytic residues.", "contents": "Modification of chymotrypsin with the specific cyanate reagent N-acetyl-p-cyanato-l-phenylalanine ethyl ester. The specific cyanate reagentN-acetyl-p-cyanato-L-phenylalanine ethyl ester (compound 1) was synthesized in an attempt to selectively modify the binding pocket of chymotrypsin (EC 3.4.21.1) while leaving the catalytic residues un touched. The reagent reacts with chymotrypsin to yield chiefly an inactive derivative 3a, with the active site Ser-195 carbamylated and the alpha-amino group, with the reagent 1 yields a modified enzyme(compound 4) with an additional carbamyl group on Ser-195.Neither derivative 3a nor 4reacts with diisopropylfluorophosphate under conditions where chymotrypsinogen is modified, indicating that Ser-195 is altered. Both derivatives 3a and 4 are retained on a 4-phenylbutylamine affinity colomn demonstrating that the substrate binding pocket is intact in both derivatives. The results indicate the potential value of aryl cyanates as protein reagents for the selective modification of nucleophilic sites. However, it is apparent that reaction at unreactive residues in the binding pocket of chymotrypsin with cyanates or similar reagents will require blockage of the more nucleophilic catalytic residues.", "PMID": 953038}
{"id": "pubmed23n0032_425", "title": "Purification and kinetic study of glyoxalase-I from rat liver, erythrocytes, brain and kidney.", "content": "Glyoxalase-I (S-lactoyl-glutathione methylglyoxal-lyase (isomerizing), EC 4.4.1.5) was purified from rat liver, erythrocytes, brain and kidney using two different purification procedures. The similarities of the purification profiles, electrophoretic mobilities and kinetics suggest that a single major form of the enzyme exists in these tissues. The highest purification (9300-fold) of the erythrocyte enzyme gave nearly homogeneous protein, molecular weight 50 000, specific activity 2410 mumol/min per mg. Kinetic studies of the rat glyoxalase-I-catalyzed disproportionation of the hemimercaptals of GSH and aromatic or aliphatic alpha-ketoaldehydes revealed broad substrate specificity with V and Km values quite insensitive to the nature of the alpha-ketoaldehydes. Use of deuterated analogs of the alpha-ketoaldhydes methylglyoxal and phenylglyoxal showed that the intramolecular hydride migration is the rate-determining step.", "contents": "Purification and kinetic study of glyoxalase-I from rat liver, erythrocytes, brain and kidney. Glyoxalase-I (S-lactoyl-glutathione methylglyoxal-lyase (isomerizing), EC 4.4.1.5) was purified from rat liver, erythrocytes, brain and kidney using two different purification procedures. The similarities of the purification profiles, electrophoretic mobilities and kinetics suggest that a single major form of the enzyme exists in these tissues. The highest purification (9300-fold) of the erythrocyte enzyme gave nearly homogeneous protein, molecular weight 50 000, specific activity 2410 mumol/min per mg. Kinetic studies of the rat glyoxalase-I-catalyzed disproportionation of the hemimercaptals of GSH and aromatic or aliphatic alpha-ketoaldehydes revealed broad substrate specificity with V and Km values quite insensitive to the nature of the alpha-ketoaldehydes. Use of deuterated analogs of the alpha-ketoaldhydes methylglyoxal and phenylglyoxal showed that the intramolecular hydride migration is the rate-determining step.", "PMID": 953039}
{"id": "pubmed23n0032_426", "title": "Mammalian adenylosuccinate synthetase. Nucleotide monophosphate substrates and inhibitors.", "content": "Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2) activity was examined in preparations from normal rat liver and a series of Morris hepatomas...", "contents": "Mammalian adenylosuccinate synthetase. Nucleotide monophosphate substrates and inhibitors. Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2) activity was examined in preparations from normal rat liver and a series of Morris hepatomas...", "PMID": 953040}
{"id": "pubmed23n0032_427", "title": "The separation of pyruvate-ferredoxin oxidoreductase from Clostridium pasteurianum into two enzymes catalyzing different reactions.", "content": "The ferredoxin requiring cleavage of pyruvate to acetyl-CoA and CO2 is catalyzed by pyruvate ferredoxin oxidoreductase (pyruvate:ferredoxin oxidoreductase (CoA-acetylating):, EC 1.2.7.1). The same enzyme is thought to catalyze the reversal of this reaction, i.e. the synthesis of pyruvate from acetyl-CoA and CO2 in the presence of reduced ferredoxin. Evidence is presented that the forward and reverse reactions are catalyzed not by one, but by two proteins that are clearly separable by Sephadex G-200 gel filtration.", "contents": "The separation of pyruvate-ferredoxin oxidoreductase from Clostridium pasteurianum into two enzymes catalyzing different reactions. The ferredoxin requiring cleavage of pyruvate to acetyl-CoA and CO2 is catalyzed by pyruvate ferredoxin oxidoreductase (pyruvate:ferredoxin oxidoreductase (CoA-acetylating):, EC 1.2.7.1). The same enzyme is thought to catalyze the reversal of this reaction, i.e. the synthesis of pyruvate from acetyl-CoA and CO2 in the presence of reduced ferredoxin. Evidence is presented that the forward and reverse reactions are catalyzed not by one, but by two proteins that are clearly separable by Sephadex G-200 gel filtration.", "PMID": 953041}
{"id": "pubmed23n0032_428", "title": "Magnetic circular dichroism of heme-isocyanide complex in aqueous media.", "content": "The nature of the ethyl isocyanide complexes of ferri- and ferro-heme was investigated in terms of the magnetic circular dichroism (MCD) spectroscopy. The complex formation of heme with ethyl isocyanide was critically dependent on the solvent system, thus, on structures of heme before addition of the ligand. The MCD spectra of the heme ethyl isocyanide complexes revealed that: (1) The addition of ethyl isocyanide to both ferri- and ferro-heme in an aqueous solution gave respective high-spin heme complexes with no more than one ethyl isocyanide molecule per one heme. (2) The ferro-heme with ethyl isocyanide in an aqueous ethanol solution formed a typical monomeric low spin ferro-hemochrome with two ligands (ethyl isocyanide) on both sides of the heme plane. (3) In the presence of excess ethyl isocyanide in an aqueous ethanol solution the ferri-heme gave an MCD spectrum similar to that for the monomeric low spin ferro-hemochrone. Therefore, ethyl isocyanide was believed to reduce the ferri-heme in an aqueous ethanol solution. (4) The ferro-heme ethyl isocyanide complex in an aqueous solution offered a Faraday B term around 450 nm instead of an apparent Faraday A term observed for the ethyl isocyanide complex of the cytochrome P-450. These results are not consistent with the view that the ferro-heme ethyl isocyanide complex in an aqueous solution can be a heme chromophore model for the cytochrome P-450 ethyl isocyanide complex.", "contents": "Magnetic circular dichroism of heme-isocyanide complex in aqueous media. The nature of the ethyl isocyanide complexes of ferri- and ferro-heme was investigated in terms of the magnetic circular dichroism (MCD) spectroscopy. The complex formation of heme with ethyl isocyanide was critically dependent on the solvent system, thus, on structures of heme before addition of the ligand. The MCD spectra of the heme ethyl isocyanide complexes revealed that: (1) The addition of ethyl isocyanide to both ferri- and ferro-heme in an aqueous solution gave respective high-spin heme complexes with no more than one ethyl isocyanide molecule per one heme. (2) The ferro-heme with ethyl isocyanide in an aqueous ethanol solution formed a typical monomeric low spin ferro-hemochrome with two ligands (ethyl isocyanide) on both sides of the heme plane. (3) In the presence of excess ethyl isocyanide in an aqueous ethanol solution the ferri-heme gave an MCD spectrum similar to that for the monomeric low spin ferro-hemochrone. Therefore, ethyl isocyanide was believed to reduce the ferri-heme in an aqueous ethanol solution. (4) The ferro-heme ethyl isocyanide complex in an aqueous solution offered a Faraday B term around 450 nm instead of an apparent Faraday A term observed for the ethyl isocyanide complex of the cytochrome P-450. These results are not consistent with the view that the ferro-heme ethyl isocyanide complex in an aqueous solution can be a heme chromophore model for the cytochrome P-450 ethyl isocyanide complex.", "PMID": 953042}
{"id": "pubmed23n0032_429", "title": "Effects of dietary molybdenum on the metabolism of copper and molybdenum in young cattle.", "content": "On separate occasions young cattle were injected intrarumenally with 99Mo or 64Cu or intravenously with 64Cu. The metabolism of the isotopes were compared to evaluate metabolic changes associated with molybdenum-induced copper deficiency. Molybdenum-99 metabolism was the same in both controls and experimental subjects. Marked differences in plasma kinetics were observed following intrarumenal 64Cu injection, with experimental animals displaying earlier plasma appearance and maintaining higher plasma levels than did controls. Similarly, higher plasma levels, more rapid plasma reappearance and greater fecal excretion were observed following intravenous injection of 64Cu in the experimental animals than in controls. A mechanism involving abnormal ceruloplasmin synthesis associated with the molybdenum-copper interaction is proposed.", "contents": "Effects of dietary molybdenum on the metabolism of copper and molybdenum in young cattle. On separate occasions young cattle were injected intrarumenally with 99Mo or 64Cu or intravenously with 64Cu. The metabolism of the isotopes were compared to evaluate metabolic changes associated with molybdenum-induced copper deficiency. Molybdenum-99 metabolism was the same in both controls and experimental subjects. Marked differences in plasma kinetics were observed following intrarumenal 64Cu injection, with experimental animals displaying earlier plasma appearance and maintaining higher plasma levels than did controls. Similarly, higher plasma levels, more rapid plasma reappearance and greater fecal excretion were observed following intravenous injection of 64Cu in the experimental animals than in controls. A mechanism involving abnormal ceruloplasmin synthesis associated with the molybdenum-copper interaction is proposed.", "PMID": 953043}
{"id": "pubmed23n0032_430", "title": "Aggregation of acridine orange: crystal structure of acridine orange tetrachlorozincate 2C17H19N3-2HCl-ZnCl2-CH3COOH.", "content": "The crystal structure of the biological stain, \"acridine orange,\" has been determined. This compound, when crystallized from ethanol, is shown to be a zinc chloride double salt of acridine orange, containing, in addition, acetic acid of crystallization. These additional components are residuals from the method of preparation of acridine orange. This complex, 2 acridine orange-2HCl-ZnCl2-CH3COOH, (2C17H19N3-2HCl-ZnCl2-CH3COOH) crystallizes in the monoclinic space group P21, a = 9.965 (2), b = 21.507 (6), c = 9.645 (2) A, beta = 113.98 degrees (2), V = 1888.7 (8) A3, FW = 800.0, Z = 2, DX = 1.41 g-cm-3, Dobs = 1.43 (9) g-cm-3. Three-dimensional diffraction data were collected with CuKalpha radiation, and the structure refined to R = 0.065 for 1885 observed reflections. In the crystal structure hydrogen bonds are formed, via the protonated nitrogen atom of the central rings of two acridine orange cations, to two chloride ions in a ZnCl42- tetrahedral grouping. These two acridine orange molecules are stacked in parallel planes, approximately 3.4 A apart, with the long axes of the ring systems inclined at 26.5 to each other. Thus an apparent dimerization of the acridine, orange is facilitated by the anions present, resulting in the complex studied. The two -N(CH3)2 groups of each acridine orange molecule are not protonated in this crystalline form. The mode of molecular packing found here may be relevant to models for the external stacking of acridine orange around a DNA molecule. The importance of removing any zinc salt from acridine orange preparations prior to aggregation studies is stressed.", "contents": "Aggregation of acridine orange: crystal structure of acridine orange tetrachlorozincate 2C17H19N3-2HCl-ZnCl2-CH3COOH. The crystal structure of the biological stain, \"acridine orange,\" has been determined. This compound, when crystallized from ethanol, is shown to be a zinc chloride double salt of acridine orange, containing, in addition, acetic acid of crystallization. These additional components are residuals from the method of preparation of acridine orange. This complex, 2 acridine orange-2HCl-ZnCl2-CH3COOH, (2C17H19N3-2HCl-ZnCl2-CH3COOH) crystallizes in the monoclinic space group P21, a = 9.965 (2), b = 21.507 (6), c = 9.645 (2) A, beta = 113.98 degrees (2), V = 1888.7 (8) A3, FW = 800.0, Z = 2, DX = 1.41 g-cm-3, Dobs = 1.43 (9) g-cm-3. Three-dimensional diffraction data were collected with CuKalpha radiation, and the structure refined to R = 0.065 for 1885 observed reflections. In the crystal structure hydrogen bonds are formed, via the protonated nitrogen atom of the central rings of two acridine orange cations, to two chloride ions in a ZnCl42- tetrahedral grouping. These two acridine orange molecules are stacked in parallel planes, approximately 3.4 A apart, with the long axes of the ring systems inclined at 26.5 to each other. Thus an apparent dimerization of the acridine, orange is facilitated by the anions present, resulting in the complex studied. The two -N(CH3)2 groups of each acridine orange molecule are not protonated in this crystalline form. The mode of molecular packing found here may be relevant to models for the external stacking of acridine orange around a DNA molecule. The importance of removing any zinc salt from acridine orange preparations prior to aggregation studies is stressed.", "PMID": 953044}
{"id": "pubmed23n0032_431", "title": "X-ray photoelectron spectroscopy of copper(II), copper(I), and mixed valence systems.", "content": "X-ray photoelectron spectroscopy using copper(II), copper(I) and the mixed valence Cu(II)/Cu(I) compounds was employed as a means  of studying electron transfer reactions in copper proteins. The X-ray photoelectron spectra of copper(II) compounds display characteristic satellites of both variable size and resolution. Some of these satellites could be assigned to specific ligand interactions. Unlike electron paramagnetic resonance spectroscopy, the X-ray photoelectron spectroscopic measurements of copper(I) compounds allowed the unequivocal assignment of this oxidation state. No satellites at all could be detected in the Cu(I) spectra. Furthermore, established mixed valence Cu(II)/Cu(I) complexes including Cu2SO3-CuSO3-2H2O and Cu4Cl5 (ethylenediamine)2 proved essentially a mixture of distinct portions of Cu(I) and Cu(II). This indicates that both oxidation states of copper survive in such complexes. In contrast, all Cu X-ray photoelectron signals of the more tentatively described mixed valence complexes Na2Cu3S3 and the mineral covellite, CuI4CuII2(S2)2S2, could be attributed exclusively to Cu(I). In view of the known binding of copper with sulfur in many copper proteins, it was of utmost importance to study the copper-sulfur interactions. We have demonstrated the absence of Cu(II) in CuS. This indicates strong metal-induced polarization of sulfur resulting in electron transfer to copper to yield Cu(I).", "contents": "X-ray photoelectron spectroscopy of copper(II), copper(I), and mixed valence systems. X-ray photoelectron spectroscopy using copper(II), copper(I) and the mixed valence Cu(II)/Cu(I) compounds was employed as a means  of studying electron transfer reactions in copper proteins. The X-ray photoelectron spectra of copper(II) compounds display characteristic satellites of both variable size and resolution. Some of these satellites could be assigned to specific ligand interactions. Unlike electron paramagnetic resonance spectroscopy, the X-ray photoelectron spectroscopic measurements of copper(I) compounds allowed the unequivocal assignment of this oxidation state. No satellites at all could be detected in the Cu(I) spectra. Furthermore, established mixed valence Cu(II)/Cu(I) complexes including Cu2SO3-CuSO3-2H2O and Cu4Cl5 (ethylenediamine)2 proved essentially a mixture of distinct portions of Cu(I) and Cu(II). This indicates that both oxidation states of copper survive in such complexes. In contrast, all Cu X-ray photoelectron signals of the more tentatively described mixed valence complexes Na2Cu3S3 and the mineral covellite, CuI4CuII2(S2)2S2, could be attributed exclusively to Cu(I). In view of the known binding of copper with sulfur in many copper proteins, it was of utmost importance to study the copper-sulfur interactions. We have demonstrated the absence of Cu(II) in CuS. This indicates strong metal-induced polarization of sulfur resulting in electron transfer to copper to yield Cu(I).", "PMID": 953045}
{"id": "pubmed23n0032_432", "title": "Paget's osteitis deformans: a carbonate-rich calcium alkali phosphate in the pre-malignant bone mineral and implications for lymphoma production by CO2.", "content": "Comparison of powder diffraction patterns of unheated normal bone mineral with the damaged by Paget's Osteitis Deformans showed that due to a difference in the spacing of one weak inner reflection the possibility exists that carbonate-containing alpha-calcium sodium orthophosphate may be a minor constituent of the osteitic bone mineral. The chemical and physical properties of this substance make it a likely minor constituent of bone salt. The presence of this calcium alkali phosphate in the osteitic mineral was tentatively proposed after demonstrating the similarity of the diffraction patterns of synthetic mixtures of this substance with tricalcium phosphate hydrate to those of the osteitic bone salt.", "contents": "Paget's osteitis deformans: a carbonate-rich calcium alkali phosphate in the pre-malignant bone mineral and implications for lymphoma production by CO2. Comparison of powder diffraction patterns of unheated normal bone mineral with the damaged by Paget's Osteitis Deformans showed that due to a difference in the spacing of one weak inner reflection the possibility exists that carbonate-containing alpha-calcium sodium orthophosphate may be a minor constituent of the osteitic bone mineral. The chemical and physical properties of this substance make it a likely minor constituent of bone salt. The presence of this calcium alkali phosphate in the osteitic mineral was tentatively proposed after demonstrating the similarity of the diffraction patterns of synthetic mixtures of this substance with tricalcium phosphate hydrate to those of the osteitic bone salt.", "PMID": 953046}
{"id": "pubmed23n0032_433", "title": "Magnetic circular dichroism of protoporphyrin derivatives in the ultraviolet region.", "content": "The MCD spectra of ferri- and ferro-heme in high and low spin states and zinc porphyrin for reference have been measured in the ultraviolet region. Zinc porphyrin offered an A term at 325 nm attributable to the N transition. A ferrous low-spin complex with CO or CN exhibited MCD spectra composed of B terms around 325 nm. This striking difference from that of zinc porphyrin in the ultraviolet region is noted from the fact that their Soret and visible MCD's gave A terms similar to those of zinc porphyrin. A ferric complex with high spin had MCD spectrum different from that of low spin, the former showing a negative C term at 287 nm and a B term at 339 nm, but the latter positive C terms at 275 nm and 320 nm. Some of these Faraday parameters were ascribed to charge-transfer transitions.", "contents": "Magnetic circular dichroism of protoporphyrin derivatives in the ultraviolet region. The MCD spectra of ferri- and ferro-heme in high and low spin states and zinc porphyrin for reference have been measured in the ultraviolet region. Zinc porphyrin offered an A term at 325 nm attributable to the N transition. A ferrous low-spin complex with CO or CN exhibited MCD spectra composed of B terms around 325 nm. This striking difference from that of zinc porphyrin in the ultraviolet region is noted from the fact that their Soret and visible MCD's gave A terms similar to those of zinc porphyrin. A ferric complex with high spin had MCD spectrum different from that of low spin, the former showing a negative C term at 287 nm and a B term at 339 nm, but the latter positive C terms at 275 nm and 320 nm. Some of these Faraday parameters were ascribed to charge-transfer transitions.", "PMID": 953047}
{"id": "pubmed23n0032_434", "title": "Reactions between nucleosides and platinum dimethylsulfoxide complexes.", "content": "Nucleosides (Nuc) react with cis-[Pt(DMSO)2Cl2] in dimethysulfoxide (DMSO) solution, to produce trans-[Pt(DMSO)(Nuc)Cl2], which then isomerize to cis-[Pt(DMSO)(Nuc)Cl2]. They also displace chlorine from K[Pt(DMSO)Cl3] in water to form trans-[Pt(DMSO)(Nuc)Cl2]. K[Pt(inosine)Cl3] and DMSO give in water cis-[Pt(DMSO)(Inosine)Cl2]. The configuration assignment of the compounds was based on chemical reactions, NMR techniques, analytical results, conductivity measurements and comparison with pyridine analogues. Chemical shifts in NMR and coupling constants of the aromatic protons of the nucleosides with 195Pt were used for the assignment of the binding sites of the nucleosides.", "contents": "Reactions between nucleosides and platinum dimethylsulfoxide complexes. Nucleosides (Nuc) react with cis-[Pt(DMSO)2Cl2] in dimethysulfoxide (DMSO) solution, to produce trans-[Pt(DMSO)(Nuc)Cl2], which then isomerize to cis-[Pt(DMSO)(Nuc)Cl2]. They also displace chlorine from K[Pt(DMSO)Cl3] in water to form trans-[Pt(DMSO)(Nuc)Cl2]. K[Pt(inosine)Cl3] and DMSO give in water cis-[Pt(DMSO)(Inosine)Cl2]. The configuration assignment of the compounds was based on chemical reactions, NMR techniques, analytical results, conductivity measurements and comparison with pyridine analogues. Chemical shifts in NMR and coupling constants of the aromatic protons of the nucleosides with 195Pt were used for the assignment of the binding sites of the nucleosides.", "PMID": 953048}
{"id": "pubmed23n0032_435", "title": "Synthesis of the monomer and dimer peptide fragments of the Micrococcus lysodeikticus cell wall peptidoglycan.", "content": "Three branched peptides, namely pentapeptide A and the two decapeptides B and C corresponding respectively to the monomer and the two dimer peptide fragments of the M. lysodeikticus cell wall peptidoglycan, were synthetized by adequate methods of peptide synthesis in homogeneous phase. The synthetized peptides showed the same electrophoretic and chromatographic behavior as the natural peptide fragments. Only decapeptide B was hydrolysed by the Myxobacter AL-1 protease by cleavage of the D-Ala-L-Ala bond and inversely, only decapeptide C was digested by the Streptomyces albus ML endopeptidase by cleavage of the D-Ala-epsilon-Lys linkage. In both enzymatic hydrolyses the pentapeptide monomer was formed.", "contents": "Synthesis of the monomer and dimer peptide fragments of the Micrococcus lysodeikticus cell wall peptidoglycan. Three branched peptides, namely pentapeptide A and the two decapeptides B and C corresponding respectively to the monomer and the two dimer peptide fragments of the M. lysodeikticus cell wall peptidoglycan, were synthetized by adequate methods of peptide synthesis in homogeneous phase. The synthetized peptides showed the same electrophoretic and chromatographic behavior as the natural peptide fragments. Only decapeptide B was hydrolysed by the Myxobacter AL-1 protease by cleavage of the D-Ala-L-Ala bond and inversely, only decapeptide C was digested by the Streptomyces albus ML endopeptidase by cleavage of the D-Ala-epsilon-Lys linkage. In both enzymatic hydrolyses the pentapeptide monomer was formed.", "PMID": 953049}
{"id": "pubmed23n0032_436", "title": "Redox regulation of cytochrome P450cam mixed function oxidation by putidaredoxin and camphor ligation.", "content": "A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cytochrome. The component interactions and electron flow are represented clearly and concisely by a free energy state diagram of the coupled binding and redox transfer reactions.", "contents": "Redox regulation of cytochrome P450cam mixed function oxidation by putidaredoxin and camphor ligation. A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cytochrome. The component interactions and electron flow are represented clearly and concisely by a free energy state diagram of the coupled binding and redox transfer reactions.", "PMID": 953050}
{"id": "pubmed23n0032_437", "title": "Varied effects of polyadenylic-polyuridylic acid on immune responses.", "content": "The effect of the double-stranded synthetic polynucleotide, poly(A).poly(U), on the immune response of inbred mouse strains to multichain synthetic polypeptides was studied. Poly(A).poly(U) did not affect immune responses controlled by H-2 linked genes. Thus, when either (T,G)-A- -L or (Phe,G)-A--L were injected into high or low responder mice followed by administration of poly(A).poly(U) 24 h after immunization, no increase in the antibody titers was observed. In contrast, poly(A).poly(U) increased significantly the response to polyproline, which is controlled by a non H-2 linked gene, in low responder mice. However, the polyribonucleotide had no effect on the antibody titers of the SJL mice, the high responders to multichain polyproline. When poly(A).poly(U) was injected into DBA/1 mice following immunization with (Phe,G)-Pro- -L, the polynucleotide enhanced the low response to the Pro- -l region at the expense of the anti (Phe,G) response which is normally high in this mouse strain. In this case poly(A).poly(U) caused an intramolecular antigenic competition. The general conclusion of this study is that the chemical nature of the antigenic determinant plays an important role in determining the type of influence exerted by poly(A).poly(U).", "contents": "Varied effects of polyadenylic-polyuridylic acid on immune responses. The effect of the double-stranded synthetic polynucleotide, poly(A).poly(U), on the immune response of inbred mouse strains to multichain synthetic polypeptides was studied. Poly(A).poly(U) did not affect immune responses controlled by H-2 linked genes. Thus, when either (T,G)-A- -L or (Phe,G)-A--L were injected into high or low responder mice followed by administration of poly(A).poly(U) 24 h after immunization, no increase in the antibody titers was observed. In contrast, poly(A).poly(U) increased significantly the response to polyproline, which is controlled by a non H-2 linked gene, in low responder mice. However, the polyribonucleotide had no effect on the antibody titers of the SJL mice, the high responders to multichain polyproline. When poly(A).poly(U) was injected into DBA/1 mice following immunization with (Phe,G)-Pro- -L, the polynucleotide enhanced the low response to the Pro- -l region at the expense of the anti (Phe,G) response which is normally high in this mouse strain. In this case poly(A).poly(U) caused an intramolecular antigenic competition. The general conclusion of this study is that the chemical nature of the antigenic determinant plays an important role in determining the type of influence exerted by poly(A).poly(U).", "PMID": 953051}
{"id": "pubmed23n0032_438", "title": "A study on the receptor for a mycobacteriophage : phage phlei.", "content": "From Mycobacterium phlei, glycolipid fractions have been isolated which inactivate phage Phlei. On the basis of the characteristics of the inactivation (specificity, kinetics, requirement for Ca++) typical of the phage-host cell system, it was concluded that these fractions contain the receptor sites for phage Phlei ; this conclusion was supported by electron microscopic studies. All the active fractions contain four kinds of components : fatty acids, glycerol, sugars (D-lyxose, 6-0-methyl-D-glucose, and low amounts of glucose and mannose), and water-soluble acids. These acids are isolated by degradation of the receptor fractions as oxalic and pyruvic acids. Variations of the ratio oxalic acid/pyruvic acid according to the mode of degradation and the absence of the peak characteristic of the protons of a pyruvic acid residue in the NMR spectrum, suggest that these acids might arise from the splitting of oxaloacetic acid. A tentative structure of the receptor is proposed, in many monoglycerides are linked through keto-acid to a polysaccharide core.", "contents": "A study on the receptor for a mycobacteriophage : phage phlei. From Mycobacterium phlei, glycolipid fractions have been isolated which inactivate phage Phlei. On the basis of the characteristics of the inactivation (specificity, kinetics, requirement for Ca++) typical of the phage-host cell system, it was concluded that these fractions contain the receptor sites for phage Phlei ; this conclusion was supported by electron microscopic studies. All the active fractions contain four kinds of components : fatty acids, glycerol, sugars (D-lyxose, 6-0-methyl-D-glucose, and low amounts of glucose and mannose), and water-soluble acids. These acids are isolated by degradation of the receptor fractions as oxalic and pyruvic acids. Variations of the ratio oxalic acid/pyruvic acid according to the mode of degradation and the absence of the peak characteristic of the protons of a pyruvic acid residue in the NMR spectrum, suggest that these acids might arise from the splitting of oxaloacetic acid. A tentative structure of the receptor is proposed, in many monoglycerides are linked through keto-acid to a polysaccharide core.", "PMID": 953052}
{"id": "pubmed23n0032_439", "title": "Grafting of enzymes on collagen films using Woodward's reagent \"K\" and a water-soluble carbodiimide derivative.", "content": "Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent \"K\" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase.", "contents": "Grafting of enzymes on collagen films using Woodward's reagent \"K\" and a water-soluble carbodiimide derivative. Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent \"K\" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase.", "PMID": 953053}
{"id": "pubmed23n0032_440", "title": "Pig prothrombin: purification and properties.", "content": "A procedure for the preparation of highly purified pig prothrombin is described. Compared to the initial clotting activity of the starting plasma, this protein was purified 776 times with a final yield of 8 per cent. The purified zymogen showed a specific activity of 1,460 NH units/mg of protein , a molecular weight of 65,000 as determined by SDS-polyacrylamide disc gel electroesis, E 1.0 mg/ml 1.0 cm, 280 nm = 1.45 at pH 7.0 and the following amino acid composition: Asx 51, Thr 38, Glx 62, Pro 23, Gly44, Ala 25, Half-Cys 30, Val 35, Met 3, Ile 30, Leu 32, Tyr 19, Phe 22, Lys 36, His 8, Arg 28, and Trp 13, which accounts for a minimum molecular weight of 59,370 (carbohydrates not computed). Alanine was found as the only N-terminal residue. Carboxypeptidases A and B failed to release any C-terminal residue. By hydrazinolysis however 0.4 mole of serine was released per mole of prothrombin. The activation of crude and chromatographed pig prothrombin was investigated.", "contents": "Pig prothrombin: purification and properties. A procedure for the preparation of highly purified pig prothrombin is described. Compared to the initial clotting activity of the starting plasma, this protein was purified 776 times with a final yield of 8 per cent. The purified zymogen showed a specific activity of 1,460 NH units/mg of protein , a molecular weight of 65,000 as determined by SDS-polyacrylamide disc gel electroesis, E 1.0 mg/ml 1.0 cm, 280 nm = 1.45 at pH 7.0 and the following amino acid composition: Asx 51, Thr 38, Glx 62, Pro 23, Gly44, Ala 25, Half-Cys 30, Val 35, Met 3, Ile 30, Leu 32, Tyr 19, Phe 22, Lys 36, His 8, Arg 28, and Trp 13, which accounts for a minimum molecular weight of 59,370 (carbohydrates not computed). Alanine was found as the only N-terminal residue. Carboxypeptidases A and B failed to release any C-terminal residue. By hydrazinolysis however 0.4 mole of serine was released per mole of prothrombin. The activation of crude and chromatographed pig prothrombin was investigated.", "PMID": 953054}
{"id": "pubmed23n0032_441", "title": "Binding of nucleotides ATP and ADP to sarcoplasmic reticulm: study by rate of dialysis.", "content": "Binding of the nucleotides ATP and ADP by preparations of sarcoplasmic reticulum was investigated by the method of flow dialysis. For ATP, experimental data could not be analyzed directly in terms of binding since a significant though small amount of hydrolysis could be observed even in presence of EDTA. ADP binding could be analyzed and gave a dissociation constant of 10-20 muM at neutral pH, and a stoichiometry of 0.35 - 0.45 per mole ATPase. The possible significance of this stoichiometry is discussed. Similar experiments were performed after ethoxyformylation of sarcoplasmic reticulum which inhibits the Ca2+ dependnet ATPase activity. The results confirmed the inhibition of ATP hydrolysis and pointed to a considerably reduced affinity for nucleotides. The method based on the measurement  of dialysis rates is convenient, and accurate enough to detect the effects of chemical modification on sarcoplasmic reticulum membranes.", "contents": "Binding of nucleotides ATP and ADP to sarcoplasmic reticulm: study by rate of dialysis. Binding of the nucleotides ATP and ADP by preparations of sarcoplasmic reticulum was investigated by the method of flow dialysis. For ATP, experimental data could not be analyzed directly in terms of binding since a significant though small amount of hydrolysis could be observed even in presence of EDTA. ADP binding could be analyzed and gave a dissociation constant of 10-20 muM at neutral pH, and a stoichiometry of 0.35 - 0.45 per mole ATPase. The possible significance of this stoichiometry is discussed. Similar experiments were performed after ethoxyformylation of sarcoplasmic reticulum which inhibits the Ca2+ dependnet ATPase activity. The results confirmed the inhibition of ATP hydrolysis and pointed to a considerably reduced affinity for nucleotides. The method based on the measurement  of dialysis rates is convenient, and accurate enough to detect the effects of chemical modification on sarcoplasmic reticulum membranes.", "PMID": 953055}
{"id": "pubmed23n0032_442", "title": "[Specificity of thermolysin action on dog myoglobin].", "content": "The proteolytic specificity of thermolysin has been studied by quantitative analysis of an enzymic digest of dog myoglobin. Results confirm main specificities of thermolysin towards Phenylalanine, Isoleucine, Leucine or Tyrosine bonds; the influence of neighbourhood was also determined and the conclusions are in a good agreement with the known structure of the active site of thermolysin.", "contents": "[Specificity of thermolysin action on dog myoglobin]. The proteolytic specificity of thermolysin has been studied by quantitative analysis of an enzymic digest of dog myoglobin. Results confirm main specificities of thermolysin towards Phenylalanine, Isoleucine, Leucine or Tyrosine bonds; the influence of neighbourhood was also determined and the conclusions are in a good agreement with the known structure of the active site of thermolysin.", "PMID": 953056}
{"id": "pubmed23n0032_443", "title": "[Chemistry of urinary mannosides excreted in mannosidosis].", "content": "Mannose-rich oligosaccharides have been isolated from urines of 5 patients with mannosidosis. Their compositon and structure were determined. Three of them have been previously described by Norden et al: alpha p-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAcp; alpha-p-Manp-(1 leads to 2), alpha-d-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAc and alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAcp, but the four others are new entities: alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp; alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp; alpha-d-Manp-(1 leads to 2), alpha-d-Man-(1 leads to 3) (alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAp and alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp. These structures are related to the glycans of \"oligomannosidic type\" present in numerous glycoproteins. All possess a N-acetylglucosamine residue in terminal reducing position and reinforce the hypothesis of Kobata et al. and Montreuil et al. that catabolism of glycans N-glucosidically linked to the protein moiety begins by the aciton of a beta-endo-N-acetylglucosaminidase.", "contents": "[Chemistry of urinary mannosides excreted in mannosidosis]. Mannose-rich oligosaccharides have been isolated from urines of 5 patients with mannosidosis. Their compositon and structure were determined. Three of them have been previously described by Norden et al: alpha p-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAcp; alpha-p-Manp-(1 leads to 2), alpha-d-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAc and alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) beta-d-Manp-(1 leads to 4) d-GlcNAcp, but the four others are new entities: alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp; alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp; alpha-d-Manp-(1 leads to 2), alpha-d-Man-(1 leads to 3) (alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAp and alpha-d-Manp-(1 leads to 2) alpha-d-Manp-(1 leads to 3) (alpha-d-Manp-(1 leads to 6) beta-d-Manp-(1 leads to 4) GlcNAcp. These structures are related to the glycans of \"oligomannosidic type\" present in numerous glycoproteins. All possess a N-acetylglucosamine residue in terminal reducing position and reinforce the hypothesis of Kobata et al. and Montreuil et al. that catabolism of glycans N-glucosidically linked to the protein moiety begins by the aciton of a beta-endo-N-acetylglucosaminidase.", "PMID": 953057}
{"id": "pubmed23n0032_444", "title": "Enzymatic properties of a new type of lysozyme isolated from Asterias rubens : comparison with the Nephthys hombergii (annelid) and hen lysozymes.", "content": "The enzymatic properties of Asterias rubens and Nephthys hombergii lysozymes were compared with those of hen egg-white lysozyme. The results allowed to conclude that the Asterias rubens lysozyme was representative of a new lysozyme type. The Nephthys hombergii lysozyme however could be classified among the hen type enzymes with a tendency to exhibit under certain conditions some properties attributed to the goose type lysozymes.", "contents": "Enzymatic properties of a new type of lysozyme isolated from Asterias rubens : comparison with the Nephthys hombergii (annelid) and hen lysozymes. The enzymatic properties of Asterias rubens and Nephthys hombergii lysozymes were compared with those of hen egg-white lysozyme. The results allowed to conclude that the Asterias rubens lysozyme was representative of a new lysozyme type. The Nephthys hombergii lysozyme however could be classified among the hen type enzymes with a tendency to exhibit under certain conditions some properties attributed to the goose type lysozymes.", "PMID": 953059}
{"id": "pubmed23n0032_445", "title": "Electrophoretic studies of non-histone proteins from pig lymph nodes.", "content": "Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histine proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in polyacrylamide gel in sodium dodecyl sulfate. Most chromatin non-histone proteins and nuclear phosphoproteins of pig lymph nodes have a molecular weight about 40 000 daltons and show a significant degree of homology of molecular range from 40 000 to 90 000 daltons. The phosphoproteins have been also analyzed by isoelectric focusing in polyacrylamide gel over the pH range of 3-10. They are distributed throughout the pI range of 5.8-9.0. The chromatin non-histone proteins and phosphoproteins are of acidic nature and contain 0,97 per cent tryptophan.", "contents": "Electrophoretic studies of non-histone proteins from pig lymph nodes. Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histine proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in polyacrylamide gel in sodium dodecyl sulfate. Most chromatin non-histone proteins and nuclear phosphoproteins of pig lymph nodes have a molecular weight about 40 000 daltons and show a significant degree of homology of molecular range from 40 000 to 90 000 daltons. The phosphoproteins have been also analyzed by isoelectric focusing in polyacrylamide gel over the pH range of 3-10. They are distributed throughout the pI range of 5.8-9.0. The chromatin non-histone proteins and phosphoproteins are of acidic nature and contain 0,97 per cent tryptophan.", "PMID": 953060}
{"id": "pubmed23n0032_446", "title": "Iron stimulation of ferritin synthesis. II.-Involvement of polysomal kc1 extracts in the mechanism of stimulation.", "content": "The iron stimulation of ferritin synthesis is a translational phenomenon. Iron acts through a modification of the microsomal apparatus as shown previously. In the present study we have shown that the factor(s) involved in this stimulation are present in the KC1 extracts from polysomes of iron-treated rats.", "contents": "Iron stimulation of ferritin synthesis. II.-Involvement of polysomal kc1 extracts in the mechanism of stimulation. The iron stimulation of ferritin synthesis is a translational phenomenon. Iron acts through a modification of the microsomal apparatus as shown previously. In the present study we have shown that the factor(s) involved in this stimulation are present in the KC1 extracts from polysomes of iron-treated rats.", "PMID": 953061}
{"id": "pubmed23n0032_447", "title": "[New syntheses of S-adenosylhomocysteine and S-adenosylmethionine analogs].", "content": "With the aim of studying analogues of S adenosyl homocysteine and S adenosyl methionine as potential inhibitors of methyl-transferases, we describe the syntheses of such analogues, in which either the amino-acid chain is replaced by various aliphatic radicals of the N 6 amino group of adenine is substituted.", "contents": "[New syntheses of S-adenosylhomocysteine and S-adenosylmethionine analogs]. With the aim of studying analogues of S adenosyl homocysteine and S adenosyl methionine as potential inhibitors of methyl-transferases, we describe the syntheses of such analogues, in which either the amino-acid chain is replaced by various aliphatic radicals of the N 6 amino group of adenine is substituted.", "PMID": 953062}
{"id": "pubmed23n0032_448", "title": "Differences in cell surface proteins of drug resistant and sensitive cell lines.", "content": "Lactoperoxidase catalyzed iodination of cell surface proteins has been used to compare theophylline and ouabain resistant variants with the drug sensitive parental cell lines. In cells showing contact inhibition a high molecular weight protein was observed to be preferentially labelled. In contrast this was not observed for non contact inhibited cells. These results support a previously suggested correlation between growth regulation and the exposure of this cell surface protein.", "contents": "Differences in cell surface proteins of drug resistant and sensitive cell lines. Lactoperoxidase catalyzed iodination of cell surface proteins has been used to compare theophylline and ouabain resistant variants with the drug sensitive parental cell lines. In cells showing contact inhibition a high molecular weight protein was observed to be preferentially labelled. In contrast this was not observed for non contact inhibited cells. These results support a previously suggested correlation between growth regulation and the exposure of this cell surface protein.", "PMID": 953063}
{"id": "pubmed23n0032_449", "title": "Effects of recurrent postnatal stresses on hypothalamic concentrations of norepinephrine.", "content": "The effect of recurrent postnatal stress on the norepinephrine content of hypothalamus has been studied. Repeated exposure to hunger or to stimulation of the  lateral nucleus of the hypothalamus served as stress-producing process. Regardless of their nature, recurrent stresses increased the norepinephrine content of the hypothalamus. The differences between controls and stressed animals were statistically significant.", "contents": "Effects of recurrent postnatal stresses on hypothalamic concentrations of norepinephrine. The effect of recurrent postnatal stress on the norepinephrine content of hypothalamus has been studied. Repeated exposure to hunger or to stimulation of the  lateral nucleus of the hypothalamus served as stress-producing process. Regardless of their nature, recurrent stresses increased the norepinephrine content of the hypothalamus. The differences between controls and stressed animals were statistically significant.", "PMID": 953097}
{"id": "pubmed23n0032_450", "title": "Prostaglandin biosynthesis in the human umbilical cord.", "content": "The content of prostaglandins (PGs) in homogenates of arteries, connective tissue from human umbilical cords, and the capacity for biosynthesis of PGs in these tissues were studied. Lipid extracts were prepared and subjected to chromatography on silicic acid columns. PGs were eluted by ethyl acetate after elution with ethyl acetate-benzene (1:9) and determined by bioassay. For studies of the content of PGs, the cords were immediately frozen after delivery and homogenized in cold ethanol. Cords taken at vaginal deliveries contained amounts equivalent to approximately 700 ng prostaglandin E2/g dry weight and cords taken at caesarean sections about 450 ng/g. After homogenization and incubation of arteries in potassium phosphate buffer at 37 degrees C for 30 min the PG content increased from about 80 to 200 ng/g. Addition of arachidonic acid increased the biosynthesis fivefold. Indomethacin inhibited it. Most of the smooth muscle stimulating activity of the homogenated cords was due to prostaglandin E compounds, but smooth muscle stimulating material similar to prostaglandin F1alpha and F2alpha was also present. Prostaglandin E2 was conclusively identified by gas chromatography -- mass spectrometry. The findings are compatible with the view that local biosynthesis of PGs is very active in the human umbilical cord after delivery.", "contents": "Prostaglandin biosynthesis in the human umbilical cord. The content of prostaglandins (PGs) in homogenates of arteries, connective tissue from human umbilical cords, and the capacity for biosynthesis of PGs in these tissues were studied. Lipid extracts were prepared and subjected to chromatography on silicic acid columns. PGs were eluted by ethyl acetate after elution with ethyl acetate-benzene (1:9) and determined by bioassay. For studies of the content of PGs, the cords were immediately frozen after delivery and homogenized in cold ethanol. Cords taken at vaginal deliveries contained amounts equivalent to approximately 700 ng prostaglandin E2/g dry weight and cords taken at caesarean sections about 450 ng/g. After homogenization and incubation of arteries in potassium phosphate buffer at 37 degrees C for 30 min the PG content increased from about 80 to 200 ng/g. Addition of arachidonic acid increased the biosynthesis fivefold. Indomethacin inhibited it. Most of the smooth muscle stimulating activity of the homogenated cords was due to prostaglandin E compounds, but smooth muscle stimulating material similar to prostaglandin F1alpha and F2alpha was also present. Prostaglandin E2 was conclusively identified by gas chromatography -- mass spectrometry. The findings are compatible with the view that local biosynthesis of PGs is very active in the human umbilical cord after delivery.", "PMID": 953098}
{"id": "pubmed23n0032_451", "title": "Dental and craniofacial development in the normal and growth-retarded human fetus.", "content": "Craniofacial and dental development were examined in 32 human fetuses, 22--42 weeks of gestational age, nine of which were classified as light-for-dates (LFD). Statistically significant differences were detected between the LFD and the normal group with respect to upper and lower jaw size and cranial base length but not in the case of neurocranial length, orbital height or deciduous molar crown size. The structures which are least affected by intrauterine growth retardation are those whose growth rate is normally either low throughout the second half of gestation or falls substantially during the 10 weeks prior to term.", "contents": "Dental and craniofacial development in the normal and growth-retarded human fetus. Craniofacial and dental development were examined in 32 human fetuses, 22--42 weeks of gestational age, nine of which were classified as light-for-dates (LFD). Statistically significant differences were detected between the LFD and the normal group with respect to upper and lower jaw size and cranial base length but not in the case of neurocranial length, orbital height or deciduous molar crown size. The structures which are least affected by intrauterine growth retardation are those whose growth rate is normally either low throughout the second half of gestation or falls substantially during the 10 weeks prior to term.", "PMID": 953099}
{"id": "pubmed23n0032_452", "title": "Topography of lesions in newborn and infant brains following cardiac arrest and resuscitation. Damage to brain and hemispheres.", "content": "The brain lesions produced by temporary arrest of circulation in a newborn and an 11-month-old infant are described. In the newborn, two periods of arrest occurred, one on the fifth day after birth and the second a few day before death on the sixth week. The older infant suffered a single episode of cardiac arrest at the age of 11 months and survived 8 days. In both cases, postmortem examination revealed lesions in spinal cord, in brain stem, and in cerebral hemispheres. This distribution of damage is compared with the patterns of injury produced experimentally by episodes of partial (hypoxia) and total (anoxia) asphyxia in subhuman primates. The coexistence of hemispheral and brain stem nuclear patterns of pathology indicates that both hypoxia and anoxia had occurred in the present cases.", "contents": "Topography of lesions in newborn and infant brains following cardiac arrest and resuscitation. Damage to brain and hemispheres. The brain lesions produced by temporary arrest of circulation in a newborn and an 11-month-old infant are described. In the newborn, two periods of arrest occurred, one on the fifth day after birth and the second a few day before death on the sixth week. The older infant suffered a single episode of cardiac arrest at the age of 11 months and survived 8 days. In both cases, postmortem examination revealed lesions in spinal cord, in brain stem, and in cerebral hemispheres. This distribution of damage is compared with the patterns of injury produced experimentally by episodes of partial (hypoxia) and total (anoxia) asphyxia in subhuman primates. The coexistence of hemispheral and brain stem nuclear patterns of pathology indicates that both hypoxia and anoxia had occurred in the present cases.", "PMID": 953100}
{"id": "pubmed23n0032_453", "title": "Relations between birth weight and learning ability in young rabbits.", "content": "Performance on visual discrimination learning was studied in 41 rabbit pups mainly in relation to their birth weight, but also as functions of their neonatal motor development and weight gain between birth and training time. Partial placental vascular ligation was performed on some fetuses to increase the number of small-for-gestational-age pups. No relation was found between learning ability and either birth weight or rate of motor development. Low birth weight showed some association with slightly delayed motor development, but resulted in no lasting motor handicap. Between two subgroups of low birth weight pups, those who showed substantial weight gain by training time learned faster than those who remained relatively small.", "contents": "Relations between birth weight and learning ability in young rabbits. Performance on visual discrimination learning was studied in 41 rabbit pups mainly in relation to their birth weight, but also as functions of their neonatal motor development and weight gain between birth and training time. Partial placental vascular ligation was performed on some fetuses to increase the number of small-for-gestational-age pups. No relation was found between learning ability and either birth weight or rate of motor development. Low birth weight showed some association with slightly delayed motor development, but resulted in no lasting motor handicap. Between two subgroups of low birth weight pups, those who showed substantial weight gain by training time learned faster than those who remained relatively small.", "PMID": 953101}
{"id": "pubmed23n0032_454", "title": "The effects of starvation, glucose infusion, and normal feeding, on muscle protein synthesis and catabolism in the newborn guinea pig.", "content": "We determined the effects of feeding, starvation, and glucose infusion after starvation in newborn guinea pigs. We determined the rate of 14C-leucine incorporation into skeletal muscle (KS) as a measure of muscle protein synthesis and the rate of excretion of 3-methylhistidine as a measure of muscle myofibrillar protein catabolism (Kc). Fed newborns, who were in positive nitrogen balance, had the highest Ks and lowest Kc, while starved newborns had the lowest Ks and highest Kc. Infusing glucose after starvation decreased net protein catabolism and Kc, but did not increase Ks. The magnitude of change of Kc in response to starvation and glucose infusion was much greater than Ks. Changes in catabolic rate may influence net muscle protein balance to a greater degree than changes in synthetic rate.", "contents": "The effects of starvation, glucose infusion, and normal feeding, on muscle protein synthesis and catabolism in the newborn guinea pig. We determined the effects of feeding, starvation, and glucose infusion after starvation in newborn guinea pigs. We determined the rate of 14C-leucine incorporation into skeletal muscle (KS) as a measure of muscle protein synthesis and the rate of excretion of 3-methylhistidine as a measure of muscle myofibrillar protein catabolism (Kc). Fed newborns, who were in positive nitrogen balance, had the highest Ks and lowest Kc, while starved newborns had the lowest Ks and highest Kc. Infusing glucose after starvation decreased net protein catabolism and Kc, but did not increase Ks. The magnitude of change of Kc in response to starvation and glucose infusion was much greater than Ks. Changes in catabolic rate may influence net muscle protein balance to a greater degree than changes in synthetic rate.", "PMID": 953102}
{"id": "pubmed23n0032_455", "title": "A transportable sleep recording system.", "content": "A system for monitoring sleep, which can be used in th ward without unduly disturbing either the patient or the medical and nursing staff, is described. The system records the electroencephalogram, electro-oculogram, the basal skin resistance and the galvanic skin resistance. In addition, the delta waves of the EEG are automatically counted and a histogram of their frequency plotted. The system allows a whole night's sleep recording to be compressed into just over a metre of paper.", "contents": "A transportable sleep recording system. A system for monitoring sleep, which can be used in th ward without unduly disturbing either the patient or the medical and nursing staff, is described. The system records the electroencephalogram, electro-oculogram, the basal skin resistance and the galvanic skin resistance. In addition, the delta waves of the EEG are automatically counted and a histogram of their frequency plotted. The system allows a whole night's sleep recording to be compressed into just over a metre of paper.", "PMID": 953118}
{"id": "pubmed23n0032_456", "title": "An active probe for electrocardiography.", "content": "In order to improve the signal to noise ratio during the recording of electrocardiographic maps from moving patients, the effect of using active electrodes with built-in amplifiers was investigated. Two kinds of electrodes using different technologies were developed: a miniature printed circuit and a thin film circuit. The first method was found to be suitable for the fabrication of small quantities in a normally equipped electronics workshop while the second, requiring highly specialised equipment, is intended for industrial manufacture in large quantities.", "contents": "An active probe for electrocardiography. In order to improve the signal to noise ratio during the recording of electrocardiographic maps from moving patients, the effect of using active electrodes with built-in amplifiers was investigated. Two kinds of electrodes using different technologies were developed: a miniature printed circuit and a thin film circuit. The first method was found to be suitable for the fabrication of small quantities in a normally equipped electronics workshop while the second, requiring highly specialised equipment, is intended for industrial manufacture in large quantities.", "PMID": 953119}
{"id": "pubmed23n0032_457", "title": "Science, statistics, and paired comparisons.", "content": "A prologue on science and statistics focuses attention on the role of statistics in science. Statisticians must be trained as scientists and to meet the needs of science. Those needs surely involve the formulation, modification and verification of stochastic models designed to represent natural phenomena. The method of paired comparisons provides a simple experimental technique but one with a literature rich in model development.", "contents": "Science, statistics, and paired comparisons. A prologue on science and statistics focuses attention on the role of statistics in science. Statisticians must be trained as scientists and to meet the needs of science. Those needs surely involve the formulation, modification and verification of stochastic models designed to represent natural phenomena. The method of paired comparisons provides a simple experimental technique but one with a literature rich in model development.", "PMID": 953129}
{"id": "pubmed23n0032_458", "title": "Composite sampling.", "content": "In sampling certain types of materials, such as bags of fertilizer, or subsampling large quantities of water, as might be done in investigating the density of plankton in certain environmental situations, it is customary to composite the samples. That is, several samples are drawn, mixed into a composite sample, and a sample taken from the composite is then used to determine relevant characteristics of the population. In this paper the results of Brown and Fisher [1972] are extended to more complex situations (e.g., more than one variance component present).", "contents": "Composite sampling. In sampling certain types of materials, such as bags of fertilizer, or subsampling large quantities of water, as might be done in investigating the density of plankton in certain environmental situations, it is customary to composite the samples. That is, several samples are drawn, mixed into a composite sample, and a sample taken from the composite is then used to determine relevant characteristics of the population. In this paper the results of Brown and Fisher [1972] are extended to more complex situations (e.g., more than one variance component present).", "PMID": 953130}
{"id": "pubmed23n0032_459", "title": "Design of experiments to estimate heritability when observations are available on parents and offspring.", "content": "The design of experiments to estimate heritability when data are available on both parents and offspring and the offspring data have a hierarchical structure is considered. Univariate maximum likelihood (ML) estimation is discussed, and extensions to the multivariate case are outlined. The efficiency of ML estimation is evaluated in cases where simple regression estimators are available. Optimum designs for ML estimation are given when various strategies of selecting and mating are followed. The variance of the heritability estimate can be approximately halved relative to designs in which no selection of parents is done.", "contents": "Design of experiments to estimate heritability when observations are available on parents and offspring. The design of experiments to estimate heritability when data are available on both parents and offspring and the offspring data have a hierarchical structure is considered. Univariate maximum likelihood (ML) estimation is discussed, and extensions to the multivariate case are outlined. The efficiency of ML estimation is evaluated in cases where simple regression estimators are available. Optimum designs for ML estimation are given when various strategies of selecting and mating are followed. The variance of the heritability estimate can be approximately halved relative to designs in which no selection of parents is done.", "PMID": 953131}
{"id": "pubmed23n0032_460", "title": "Models allowing for age-dependent survival rates for band-return data.", "content": "Seber [1970] and Robson and Youngs [1971] developed a statistical model for a tag-recapture experiment on an exploited animal population in which banded or tagged individuals are released into a population each year, and the bands from harvested individuals are returned by hunters or fishermen in following years. Consideration of certain biological and ecologicw models for an experimental situation where data are recorded separately for birds released as adults and as young-of-the-year. In particular, the effect of different survival rates of young birds, and the effect of migration on reporting rates, are taken into account in the models presented in this study. Maximum likelihood estimators of parameters are derived under the different models, and procedures are developed to discriminate between alternative models as well as provide non-discriminant goodness-of-fit tests.", "contents": "Models allowing for age-dependent survival rates for band-return data. Seber [1970] and Robson and Youngs [1971] developed a statistical model for a tag-recapture experiment on an exploited animal population in which banded or tagged individuals are released into a population each year, and the bands from harvested individuals are returned by hunters or fishermen in following years. Consideration of certain biological and ecologicw models for an experimental situation where data are recorded separately for birds released as adults and as young-of-the-year. In particular, the effect of different survival rates of young birds, and the effect of migration on reporting rates, are taken into account in the models presented in this study. Maximum likelihood estimators of parameters are derived under the different models, and procedures are developed to discriminate between alternative models as well as provide non-discriminant goodness-of-fit tests.", "PMID": 953132}
{"id": "pubmed23n0032_461", "title": "Mathematical models for nonparametric inferences from line transect data.", "content": "A general mathematical theory of line transects is develoepd which supplies a framework for nonparametric density estimation based on either right angle or sighting distances. The probability of observing a point given its right angle distance (y) from the line is generalized to an arbitrary function g(y). Given only that g(O) = 1, it is shown there are nonparametric approaches to density estimation using the observed right angle distances. The model is then generalized to include sighting distances (r). Let f(y/r) be the conditional distribution of right angle distance given sighting distance. It is shown that nonparametric estimation based only on sighting distances requires we know the transformation of r given by f(O/r).", "contents": "Mathematical models for nonparametric inferences from line transect data. A general mathematical theory of line transects is develoepd which supplies a framework for nonparametric density estimation based on either right angle or sighting distances. The probability of observing a point given its right angle distance (y) from the line is generalized to an arbitrary function g(y). Given only that g(O) = 1, it is shown there are nonparametric approaches to density estimation using the observed right angle distances. The model is then generalized to include sighting distances (r). Let f(y/r) be the conditional distribution of right angle distance given sighting distance. It is shown that nonparametric estimation based only on sighting distances requires we know the transformation of r given by f(O/r).", "PMID": 953133}
{"id": "pubmed23n0032_462", "title": "A statistical model of the dynamics of a mosquito vector (Culex tarsalis) population.", "content": "A model of the dynamics of a mosquito Culex tarsalis is derived that includes the life states through which the mosquito proceeds. Transition probabilities from one state (egg, larva, pupa and adult) to another are derived and they depend on the duration of stay and mortality in each state. A formula is derived for the expected number of mosquitoes alive at any time during the spring or summer. This formula depends on the number of eggs oviposited and the transition probabilities. Data are used to estimate the parameters and to illustrate the usefulness of this model in examining the effect of changes in mosquito survival on the dynamics of the population.", "contents": "A statistical model of the dynamics of a mosquito vector (Culex tarsalis) population. A model of the dynamics of a mosquito Culex tarsalis is derived that includes the life states through which the mosquito proceeds. Transition probabilities from one state (egg, larva, pupa and adult) to another are derived and they depend on the duration of stay and mortality in each state. A formula is derived for the expected number of mosquitoes alive at any time during the spring or summer. This formula depends on the number of eggs oviposited and the transition probabilities. Data are used to estimate the parameters and to illustrate the usefulness of this model in examining the effect of changes in mosquito survival on the dynamics of the population.", "PMID": 953134}
{"id": "pubmed23n0032_463", "title": "Estimating the rate constants in a two-compartment stochastic model.", "content": "A procedure is described for estimating the rate constants of a two-compartment stochastic model for which the covariance structure over time of the observations is known. The proposed estimation procedure, by incorporating the known (as a function of the parameters to be estimated) covariance structure of the observations, produces regular best asymptotically normal (RBAN) estimators for the parameters. In addition, the construction of approximate confidence intervals and regions for the parameters is made possible by identification of the asymptotic covariance matrix of the estimators. The explicit form of the inverse of the covariance matrix, which is required in the estimation procedure, is presented. The procedure is illustrated by application to real as well as simulated data, and a comparison is made to the widely used nonlinear least squares procedure, which does not account for correlations over time.", "contents": "Estimating the rate constants in a two-compartment stochastic model. A procedure is described for estimating the rate constants of a two-compartment stochastic model for which the covariance structure over time of the observations is known. The proposed estimation procedure, by incorporating the known (as a function of the parameters to be estimated) covariance structure of the observations, produces regular best asymptotically normal (RBAN) estimators for the parameters. In addition, the construction of approximate confidence intervals and regions for the parameters is made possible by identification of the asymptotic covariance matrix of the estimators. The explicit form of the inverse of the covariance matrix, which is required in the estimation procedure, is presented. The procedure is illustrated by application to real as well as simulated data, and a comparison is made to the widely used nonlinear least squares procedure, which does not account for correlations over time.", "PMID": 953135}
{"id": "pubmed23n0032_464", "title": "A test for randomness.", "content": "A procedure is proposed for studying the hypothesis that a sequence of dichotomous random variables are independent and identically (randomly) distributed. The procedure is relatively sensitive to departures from randomness involving multiple clustering. An application to testing for Schwann cell disease is discussed. The distrubution of the sample coefficient of variation from an exponential distribution, a useful statistic in testing randomness for a continuous model, is also studied.", "contents": "A test for randomness. A procedure is proposed for studying the hypothesis that a sequence of dichotomous random variables are independent and identically (randomly) distributed. The procedure is relatively sensitive to departures from randomness involving multiple clustering. An application to testing for Schwann cell disease is discussed. The distrubution of the sample coefficient of variation from an exponential distribution, a useful statistic in testing randomness for a continuous model, is also studied.", "PMID": 953136}
{"id": "pubmed23n0032_465", "title": "Regression analysis of the log odds ratio: a method for retrospective studies.", "content": "Quantification of the dependence of the odds ratio on concomitant variables associated with each of several 2 X 2 tables, using a regression model proposed by Zelen [1971], is an important tool for retrospective studies in epidemiology, An \"exact\" analysis may be based on the conditional likelihood obtained by fixing all the marginal totals. A symptotically this approach is equivalent to use of an unconditional log-linear model. The method is used to reanalyze data reported by Kneale [1971] on the relationship between obstetric radiation and childhood cancer.", "contents": "Regression analysis of the log odds ratio: a method for retrospective studies. Quantification of the dependence of the odds ratio on concomitant variables associated with each of several 2 X 2 tables, using a regression model proposed by Zelen [1971], is an important tool for retrospective studies in epidemiology, An \"exact\" analysis may be based on the conditional likelihood obtained by fixing all the marginal totals. A symptotically this approach is equivalent to use of an unconditional log-linear model. The method is used to reanalyze data reported by Kneale [1971] on the relationship between obstetric radiation and childhood cancer.", "PMID": 953137}
{"id": "pubmed23n0032_466", "title": "Power spectrum of the respiratory system.", "content": "Spectrum analysis is proposed as a method for analyzing biologic time series data that arise in the study of respiration. A mechanical model of the respiratory system is used to provide a theoretical basis for the interpretation of the air flow spectrum in terms of a physically defined energy concept. We show that under certain conditions the flow variance is proportional to the mechanical work of breathing, and indicate how this new parameter is related to conventional measures of respiratory function. An example is presented to describe the computational procedure and to illustrate the graphical aspects of cross-spectrum analysis.", "contents": "Power spectrum of the respiratory system. Spectrum analysis is proposed as a method for analyzing biologic time series data that arise in the study of respiration. A mechanical model of the respiratory system is used to provide a theoretical basis for the interpretation of the air flow spectrum in terms of a physically defined energy concept. We show that under certain conditions the flow variance is proportional to the mechanical work of breathing, and indicate how this new parameter is related to conventional measures of respiratory function. An example is presented to describe the computational procedure and to illustrate the graphical aspects of cross-spectrum analysis.", "PMID": 953138}
{"id": "pubmed23n0032_467", "title": "The analysis of ranked data derived from completely randomized factorial designs.", "content": "This paper presents a method for the analysis of ranked data arising from completely randomized factorial designs. The procedure, which is an extension of the Kruskal-Wallis ranks test, allows for the calculation of interaction effects and linear contrasts. A Monte Carlo study of the convergence of the test and a worked example are presented.", "contents": "The analysis of ranked data derived from completely randomized factorial designs. This paper presents a method for the analysis of ranked data arising from completely randomized factorial designs. The procedure, which is an extension of the Kruskal-Wallis ranks test, allows for the calculation of interaction effects and linear contrasts. A Monte Carlo study of the convergence of the test and a worked example are presented.", "PMID": 953139}
{"id": "pubmed23n0032_468", "title": "Stability of population growth determined by 2 X 2 Leslie matrix with density-dependent elements.", "content": "The matrix considered contains four elements, each a function of total number. A special case for which the matrix may be appropriate is when the population may be divided into juveniles and adults, and the survival rates and fecundity are the same for all members of each group. This is true, at least approximatley, for many species of birds. The conditions for stability are determined and are illustrated with a model based on the data collected on the Marley Wood, Oxford, population of the Great tit (Parus major L.). A reformulation of the conditons permits comparison with the conditions for a population with identical survival rates and fecundity for all age groups, i.e., a population without effective age structure. Conditions under which the age structure may be ignored when discussing stability are considered.", "contents": "Stability of population growth determined by 2 X 2 Leslie matrix with density-dependent elements. The matrix considered contains four elements, each a function of total number. A special case for which the matrix may be appropriate is when the population may be divided into juveniles and adults, and the survival rates and fecundity are the same for all members of each group. This is true, at least approximatley, for many species of birds. The conditions for stability are determined and are illustrated with a model based on the data collected on the Marley Wood, Oxford, population of the Great tit (Parus major L.). A reformulation of the conditons permits comparison with the conditions for a population with identical survival rates and fecundity for all age groups, i.e., a population without effective age structure. Conditions under which the age structure may be ignored when discussing stability are considered.", "PMID": 953140}
{"id": "pubmed23n0032_469", "title": "Expectations for inbreeding depression on self-fertilization of tetraploids.", "content": "The contribution to the inbreeding depression from a digenic tetrasomic locus upon self-fertilization involves three genotypic interaction effects which may be thought of as a generalization of the dominance deviation for a diploid locus. It is shown how this contribution may be expressed in terms of these genotypic interaction effects, the gene frequencies and the number of generations of selfing.", "contents": "Expectations for inbreeding depression on self-fertilization of tetraploids. The contribution to the inbreeding depression from a digenic tetrasomic locus upon self-fertilization involves three genotypic interaction effects which may be thought of as a generalization of the dominance deviation for a diploid locus. It is shown how this contribution may be expressed in terms of these genotypic interaction effects, the gene frequencies and the number of generations of selfing.", "PMID": 953141}
{"id": "pubmed23n0032_470", "title": "Reanalysis of some baboon descent data.", "content": "Wagner and Altmann [1973] recently reported an analysis of a set of baboon descent times, some of which were left censored due to varying arrival times at the observation site. Any transformation of the data which reverses order produced a set of right censored observations. In this way, the Kaplan-Meier [1958] estimate of the descent time distribution can be computed. Estimates of the mean and variance of the distribution are given, as well as the standard error of the estimate of the mean.", "contents": "Reanalysis of some baboon descent data. Wagner and Altmann [1973] recently reported an analysis of a set of baboon descent times, some of which were left censored due to varying arrival times at the observation site. Any transformation of the data which reverses order produced a set of right censored observations. In this way, the Kaplan-Meier [1958] estimate of the descent time distribution can be computed. Estimates of the mean and variance of the distribution are given, as well as the standard error of the estimate of the mean.", "PMID": 953142}
{"id": "pubmed23n0032_471", "title": "A generalization of a matrix occupancy problem.", "content": "An extension of the so-called committee problem is discussed in terms of the occupancy of a certain matrix whose row totals are taken as fixed, and whose cell entries are constrained to lie between zero and defined maxima. A statistic is constructed as a function of the column totals and used to test the significance of a column effect, irrespective of row differences. Two special cases when the cell maxima are constant, first within rows and second within columns, yield a covariance matrix for the column totals whose inverse may be found explicitly, and this in turn leads to a relatively simple expression for the test statistic. Applications of this generalized problem to certain health research situations are discussed, and the adequacy of a X2 approximation to the statistic's null distribution is mentioned.", "contents": "A generalization of a matrix occupancy problem. An extension of the so-called committee problem is discussed in terms of the occupancy of a certain matrix whose row totals are taken as fixed, and whose cell entries are constrained to lie between zero and defined maxima. A statistic is constructed as a function of the column totals and used to test the significance of a column effect, irrespective of row differences. Two special cases when the cell maxima are constant, first within rows and second within columns, yield a covariance matrix for the column totals whose inverse may be found explicitly, and this in turn leads to a relatively simple expression for the test statistic. Applications of this generalized problem to certain health research situations are discussed, and the adequacy of a X2 approximation to the statistic's null distribution is mentioned.", "PMID": 953143}
{"id": "pubmed23n0032_472", "title": "Effect of ultrasound on the permeability of D2O through cellulose membranes.", "content": "The effect of ultrasound energy on the permeability of water through cellulose membranes was studied using deuterium oxide (D2O) as a tracer. The same membrane was used for control (no ultrasound) and ultrasound experiments in a sequential reversal design. Transfer of D2O to a 2% albumin solution was measured in a glass dializing cell with membrane area of 11 cm2. Ultrasonic energy was supplied for 45 min by a hydrosonic bath (Linden Laboratories) at an intensity of 0.18 watt/cm2, an energy level commonly used for therapeutic purposes. Statistical analysis showed a significant increase in water transfer due to the ultrasound treatment irrespective of the sequence of application of ultrasound. It was concluded that ultrasound energy increases water permeability through cellulose dializing membranes. Whether or not increased water permeability is the operating mechanism underlying the beneficial effects of ultrasonic bath therapy remains unanswered.", "contents": "Effect of ultrasound on the permeability of D2O through cellulose membranes. The effect of ultrasound energy on the permeability of water through cellulose membranes was studied using deuterium oxide (D2O) as a tracer. The same membrane was used for control (no ultrasound) and ultrasound experiments in a sequential reversal design. Transfer of D2O to a 2% albumin solution was measured in a glass dializing cell with membrane area of 11 cm2. Ultrasonic energy was supplied for 45 min by a hydrosonic bath (Linden Laboratories) at an intensity of 0.18 watt/cm2, an energy level commonly used for therapeutic purposes. Statistical analysis showed a significant increase in water transfer due to the ultrasound treatment irrespective of the sequence of application of ultrasound. It was concluded that ultrasound energy increases water permeability through cellulose dializing membranes. Whether or not increased water permeability is the operating mechanism underlying the beneficial effects of ultrasonic bath therapy remains unanswered.", "PMID": 953146}
{"id": "pubmed23n0032_473", "title": "Effect of resorbable calcium aluminate ceramics on regulation of calcium and phosphorus in rats.", "content": "Ions released from resorbable ceramics could be toxic to the animal. Experiments were designed to study the effect of implanting three different weights of porous resorbable calcium aluminate ceramics (0.172, 0.332, and 0.504 g) in rats for a total duration of 300 days. Gross and microscopic examination of heart, liver, kidneys, trachea with thyroid, and muscle adjacent to the implant did not show any pathological changes. Calcium and inorganic phosphate content of bone, serum and urine were not affected by the implants. Urine hydroxyproline excretion did not change in the animals implanted with ceramics. Animals implanted with 0.332 g of ceramics had a significantly higher serum alkaline phosphatase activity than the control animals. Resorption of calcium and depositon of inorganic phosphates in the implanted ceramics suggested that ions were being exchanged with the body fluids. Implantation of 0.172 to 0.332 g porous resorbable calcium aluminate ceramic was not toxic to the animal.", "contents": "Effect of resorbable calcium aluminate ceramics on regulation of calcium and phosphorus in rats. Ions released from resorbable ceramics could be toxic to the animal. Experiments were designed to study the effect of implanting three different weights of porous resorbable calcium aluminate ceramics (0.172, 0.332, and 0.504 g) in rats for a total duration of 300 days. Gross and microscopic examination of heart, liver, kidneys, trachea with thyroid, and muscle adjacent to the implant did not show any pathological changes. Calcium and inorganic phosphate content of bone, serum and urine were not affected by the implants. Urine hydroxyproline excretion did not change in the animals implanted with ceramics. Animals implanted with 0.332 g of ceramics had a significantly higher serum alkaline phosphatase activity than the control animals. Resorption of calcium and depositon of inorganic phosphates in the implanted ceramics suggested that ions were being exchanged with the body fluids. Implantation of 0.172 to 0.332 g porous resorbable calcium aluminate ceramic was not toxic to the animal.", "PMID": 953147}
{"id": "pubmed23n0032_474", "title": "2,3-Diphosphoglycerate red cell concentration changes during the menstrual cycle in woman.", "content": "The authors showed that 2,3-DPG red cell concentration (2,3-DPG) WAS SIGNIFICANTLY HIGHER DURING THE LAST WEEK Of the menstrual cycle of five women (1.08+/-0.09 mol-,p;Hb-1) (M +/- 1 SE) THAN DURING THE First part and the last but one week of the cycle (0.86+/-0.04 mol-molHb-1, p less than 0.01). PACO2 was significantly lower during the last week of the cycle (36.3+/-1.3 torr) than during the first part and the last but one week of the cycle(40.1+/-0.8 torr, p less than 0.001). This hypocapnia was due to transient hyperventilation in the second part of the menstrual cycle. 5 men were studied during 4 weeks to determinate control values. No change was observed. The 2,3-DGP mean value (1.10+/-0.01 mol-molGb-1) was not significantly different of the values observed in the women during the last week of the cycle. These results suggest that the 2,3-DPG increase during the last week of the menstrual cylce is problably due to a respiratory alkalosis induced by progesterone secretion.", "contents": "2,3-Diphosphoglycerate red cell concentration changes during the menstrual cycle in woman. The authors showed that 2,3-DPG red cell concentration (2,3-DPG) WAS SIGNIFICANTLY HIGHER DURING THE LAST WEEK Of the menstrual cycle of five women (1.08+/-0.09 mol-,p;Hb-1) (M +/- 1 SE) THAN DURING THE First part and the last but one week of the cycle (0.86+/-0.04 mol-molHb-1, p less than 0.01). PACO2 was significantly lower during the last week of the cycle (36.3+/-1.3 torr) than during the first part and the last but one week of the cycle(40.1+/-0.8 torr, p less than 0.001). This hypocapnia was due to transient hyperventilation in the second part of the menstrual cycle. 5 men were studied during 4 weeks to determinate control values. No change was observed. The 2,3-DGP mean value (1.10+/-0.01 mol-molGb-1) was not significantly different of the values observed in the women during the last week of the cycle. These results suggest that the 2,3-DPG increase during the last week of the menstrual cylce is problably due to a respiratory alkalosis induced by progesterone secretion.", "PMID": 953148}
{"id": "pubmed23n0032_475", "title": "A case of hemoglobin M Boston. New data about valency hybrids brought by isoelectrofocusing study.", "content": "In Hemoglobins M, as the result of a mutation theheme iron of the abnormal chain is atabilized in an oxidized form unable to carry oxygen. In this paper, we discuss the case of hemoglobin M Boston characterizing this abnormal hemoglobin as valency hybrid by spectral studies and utilising this mutant as a marker, in isoelectricfocusing, we bring the arguments that the two intermediary bands of oxidation correspond to alpha2+beta2+2 and alpha2+2beta2+3 We also indicate that the abnormal spectrum of Hb M interferes with the estimation of methemoglobin, giving erroneous values. We have shown that isoelectric focusing and subsequent scanning give a definite idea about the amount of abnormal chain present.", "contents": "A case of hemoglobin M Boston. New data about valency hybrids brought by isoelectrofocusing study. In Hemoglobins M, as the result of a mutation theheme iron of the abnormal chain is atabilized in an oxidized form unable to carry oxygen. In this paper, we discuss the case of hemoglobin M Boston characterizing this abnormal hemoglobin as valency hybrid by spectral studies and utilising this mutant as a marker, in isoelectricfocusing, we bring the arguments that the two intermediary bands of oxidation correspond to alpha2+beta2+2 and alpha2+2beta2+3 We also indicate that the abnormal spectrum of Hb M interferes with the estimation of methemoglobin, giving erroneous values. We have shown that isoelectric focusing and subsequent scanning give a definite idea about the amount of abnormal chain present.", "PMID": 953149}
{"id": "pubmed23n0032_476", "title": "Electrostatic free energy and shift of the phase transition for charged lipid membranes.", "content": "For a charged membrane in an electrolyte solution the electrostatic free energy is derived treating the system as a diffuse double layer. The dependence of the energy on external parameters like surface charge density and temperature is obtained and the physical basis discussed. As an application the charges are shown to exert an electrostatic surface pressure on the lipid chain packing which leads to a shift in the phase transition of membranes. The results confirm the interpretation of experimental data as given by Tr\u00e4uble et al. in the accompanying paper.", "contents": "Electrostatic free energy and shift of the phase transition for charged lipid membranes. For a charged membrane in an electrolyte solution the electrostatic free energy is derived treating the system as a diffuse double layer. The dependence of the energy on external parameters like surface charge density and temperature is obtained and the physical basis discussed. As an application the charges are shown to exert an electrostatic surface pressure on the lipid chain packing which leads to a shift in the phase transition of membranes. The results confirm the interpretation of experimental data as given by Tr\u00e4uble et al. in the accompanying paper.", "PMID": 953150}
{"id": "pubmed23n0032_477", "title": "Differential hydrogen ion titrations of the histidine residues in Helix pomatia haemocyanin.", "content": "The properties of the histidine residues in Helix pomatia haemocyanin have been studied by differential hydrogen ion titrations. In oxy-and deoxyhaemocyanin 31 X 10(-5) histidine residues per g protein are titrated in contrast to 35 X 10(-5) residues in apohaemocyanin. The difference corresponds to a stoichiometry of one histidine residue per copper atom bound. Even in apohaemocyanin about 6 X 10(-5) histidine residues per  g protein are not titrated in their normal pH region. In the presence of sufficient calcium to displace the dissociation completely out of the titration region, the titration curve of apohaemocyanin could be linarized according to the model of Linderstrom--Lang. In oxy-and deoxyhaemocyanin, however, a distinct deviation from linearity was found under the same conditions. In the absence of calcium the effect of the dissociation adds up to this deviation. The electrostatic interaction factors were determined for the protein at 0.1 M KC1 and for the dissociation products: halves and tenths at 1.0 M KC1. The electrostatic interaction factor for the wholes and the halves are much smaller than the values calculated from the Linderstrom--Lang equation, using the radius of the equivalent sphere either obtained from electron microscopy or from the partial specific volume. This probably due to solvent penetration. For the tenths at 1.0 M KC1, this effect is small.", "contents": "Differential hydrogen ion titrations of the histidine residues in Helix pomatia haemocyanin. The properties of the histidine residues in Helix pomatia haemocyanin have been studied by differential hydrogen ion titrations. In oxy-and deoxyhaemocyanin 31 X 10(-5) histidine residues per g protein are titrated in contrast to 35 X 10(-5) residues in apohaemocyanin. The difference corresponds to a stoichiometry of one histidine residue per copper atom bound. Even in apohaemocyanin about 6 X 10(-5) histidine residues per  g protein are not titrated in their normal pH region. In the presence of sufficient calcium to displace the dissociation completely out of the titration region, the titration curve of apohaemocyanin could be linarized according to the model of Linderstrom--Lang. In oxy-and deoxyhaemocyanin, however, a distinct deviation from linearity was found under the same conditions. In the absence of calcium the effect of the dissociation adds up to this deviation. The electrostatic interaction factors were determined for the protein at 0.1 M KC1 and for the dissociation products: halves and tenths at 1.0 M KC1. The electrostatic interaction factor for the wholes and the halves are much smaller than the values calculated from the Linderstrom--Lang equation, using the radius of the equivalent sphere either obtained from electron microscopy or from the partial specific volume. This probably due to solvent penetration. For the tenths at 1.0 M KC1, this effect is small.", "PMID": 953151}
{"id": "pubmed23n0032_478", "title": "Ion binding by X-537A. Equilibrium and rate of complexation of Co(2+) in methanol.", "content": "The reaction of X-537A (XH) with Co(2+) in methanol to form the complex CoX(+) has been studied fluorometrically to determine the equilibrium constant as a function of temperature. The effect of complexation on the proton NMR spectrum of the X-537A was studied to determine the kinetics of complex formation. Comparing the data for the reaction XM(+) leads to X(-) + M(2+) in methanol at 25 degrees for several M(2+) we find that the equilibrium constants increase in the order CoX(+) less than MnX(+) and span only a factor of 5 while the rate constants increase in the order NiX(+) less than CoX(+) less than MnX(+) and span a factor of more than 100.", "contents": "Ion binding by X-537A. Equilibrium and rate of complexation of Co(2+) in methanol. The reaction of X-537A (XH) with Co(2+) in methanol to form the complex CoX(+) has been studied fluorometrically to determine the equilibrium constant as a function of temperature. The effect of complexation on the proton NMR spectrum of the X-537A was studied to determine the kinetics of complex formation. Comparing the data for the reaction XM(+) leads to X(-) + M(2+) in methanol at 25 degrees for several M(2+) we find that the equilibrium constants increase in the order CoX(+) less than MnX(+) and span only a factor of 5 while the rate constants increase in the order NiX(+) less than CoX(+) less than MnX(+) and span a factor of more than 100.", "PMID": 953152}
{"id": "pubmed23n0032_479", "title": "Association kinetics with coupled diffusional flows. Special application to the lac repressor--operator system.", "content": "The time development of the association of the lac repressor to the operator is considered in a model where the repressor is allowed to bind unspecifically to DNA and move along the DNA chain in a one-dimensional diffusion. The coupling to the three-dimensional diffusion outside the chain is introduced by letting the repressor associate and dissociate from the chain until it is finally bound to the operator. All distance correlations along the chain are included. The mean time of association is calculated and through a comparison with experimental data the molecular parameters are determined. The one-dimensional diffusion constant is found to be of the order of 10(-9) cm(2)s(-1). The model is sufficiently general to be applicable to other similar systems.", "contents": "Association kinetics with coupled diffusional flows. Special application to the lac repressor--operator system. The time development of the association of the lac repressor to the operator is considered in a model where the repressor is allowed to bind unspecifically to DNA and move along the DNA chain in a one-dimensional diffusion. The coupling to the three-dimensional diffusion outside the chain is introduced by letting the repressor associate and dissociate from the chain until it is finally bound to the operator. All distance correlations along the chain are included. The mean time of association is calculated and through a comparison with experimental data the molecular parameters are determined. The one-dimensional diffusion constant is found to be of the order of 10(-9) cm(2)s(-1). The model is sufficiently general to be applicable to other similar systems.", "PMID": 953153}
{"id": "pubmed23n0032_480", "title": "Thermodynamic and kinetic parameters of ion condensation to polynucleotides. Outer sphere complex formed by Mg++ions.", "content": "The coupling of ion binding to the single strand helix-coil transition in poly (A) and poly (C) is used to obtain information about both processes by ion titration and field-jump relaxation methods. Characterisation of the field-jump relaxation in poly (C) at various concentrations of monovalent ions leads to the evaluation of a stability constant K = 71 M(-1) for the ion binding to the polymer. the rate constant of helix formation is found to be 1.3 X 10(7)s(-1), whereas the dissociation rate is 1.0 X 10(6)s(-1). Similar data are preseented for poly (A) and poly (dA). The interaction of Mg(++) and Ca(++) with poly (A) and poly (C) is measured by a titration method using the polymer absorbance for the indication of binding. The data can be represented by a model with independent binding \"sites\". The stability constants increase with decreasing salt concentration from 2.7 X 10(4) M(-1) at medium ionic strengths up to 2.7 X 10(7) M(-1) at low ionic strength. The number of ions bound per nucleotide residue is in the range 0.2 to 0.3. Relaxation time constants associated with Mg(++) binding are characterised over a broad range of Mg(++) concentrations form 5 muM to 500 muM. The observed concentration dependence supports the conclusion on the number of binding places inferred from equilibrium titrations. The rate of Mg(++) and Ca(++) association to the polymer is close to the limit of diffusion control kR =1 X 10(10) to 2 X 10(10) M(-1) s(-1)). This high rate demonstrates that Mg(++) and Ca(++) ions do not form inner-sphere complexes with the polynucleotides. Apparently the distance between two adjacent phosphates is too large for a simultaneous site binding of Mg(++) or Ca(++), and inner sphere complexation at a single phosphate seems to be too weak. The data support the view that the ions like Mg(++) and Ca(++) surround the polynucleotides in the form of a mobile ion cloud without site binding.", "contents": "Thermodynamic and kinetic parameters of ion condensation to polynucleotides. Outer sphere complex formed by Mg++ions. The coupling of ion binding to the single strand helix-coil transition in poly (A) and poly (C) is used to obtain information about both processes by ion titration and field-jump relaxation methods. Characterisation of the field-jump relaxation in poly (C) at various concentrations of monovalent ions leads to the evaluation of a stability constant K = 71 M(-1) for the ion binding to the polymer. the rate constant of helix formation is found to be 1.3 X 10(7)s(-1), whereas the dissociation rate is 1.0 X 10(6)s(-1). Similar data are preseented for poly (A) and poly (dA). The interaction of Mg(++) and Ca(++) with poly (A) and poly (C) is measured by a titration method using the polymer absorbance for the indication of binding. The data can be represented by a model with independent binding \"sites\". The stability constants increase with decreasing salt concentration from 2.7 X 10(4) M(-1) at medium ionic strengths up to 2.7 X 10(7) M(-1) at low ionic strength. The number of ions bound per nucleotide residue is in the range 0.2 to 0.3. Relaxation time constants associated with Mg(++) binding are characterised over a broad range of Mg(++) concentrations form 5 muM to 500 muM. The observed concentration dependence supports the conclusion on the number of binding places inferred from equilibrium titrations. The rate of Mg(++) and Ca(++) association to the polymer is close to the limit of diffusion control kR =1 X 10(10) to 2 X 10(10) M(-1) s(-1)). This high rate demonstrates that Mg(++) and Ca(++) ions do not form inner-sphere complexes with the polynucleotides. Apparently the distance between two adjacent phosphates is too large for a simultaneous site binding of Mg(++) or Ca(++), and inner sphere complexation at a single phosphate seems to be too weak. The data support the view that the ions like Mg(++) and Ca(++) surround the polynucleotides in the form of a mobile ion cloud without site binding.", "PMID": 953154}
{"id": "pubmed23n0032_481", "title": "The measure-unit of evolution.", "content": "A theoretical study on evolution has been carried out, with the aim of disproving some value judgements essentially represented by the idea that the degree of evolution can increase. Our conclusions are based upon the preliminary statement that efficiency of survival is directly related to regulative ability. On this ground our reasoning led us to conclude that: (i) actual fitness measure units derive from an anthropocentric bias, and they mainly evaluate similarity to man rather than some objective parameter; (ii) a complete and meaningful unit is, at present, impossible to achieve in practice; (iii) since the study of evolution is only descriptive, and since the evolutionary process is time dependent, every ecological dominant living today must be considered as the most fitted to its environment; (iv) the view we can have of evolution is simply a transection, so that many generalized phyletic trees are trivial and it is impossible to claim the persistence today of those \"ancestor organisms\" upon which such trees are constructed. Moreover, a functional definition of the term \"organism\" is given, following criteria drawn from bioenergetics and from biological hierarchization. The concluding step is the assemblage of a slightly heterodox model for evolution.", "contents": "The measure-unit of evolution. A theoretical study on evolution has been carried out, with the aim of disproving some value judgements essentially represented by the idea that the degree of evolution can increase. Our conclusions are based upon the preliminary statement that efficiency of survival is directly related to regulative ability. On this ground our reasoning led us to conclude that: (i) actual fitness measure units derive from an anthropocentric bias, and they mainly evaluate similarity to man rather than some objective parameter; (ii) a complete and meaningful unit is, at present, impossible to achieve in practice; (iii) since the study of evolution is only descriptive, and since the evolutionary process is time dependent, every ecological dominant living today must be considered as the most fitted to its environment; (iv) the view we can have of evolution is simply a transection, so that many generalized phyletic trees are trivial and it is impossible to claim the persistence today of those \"ancestor organisms\" upon which such trees are constructed. Moreover, a functional definition of the term \"organism\" is given, following criteria drawn from bioenergetics and from biological hierarchization. The concluding step is the assemblage of a slightly heterodox model for evolution.", "PMID": 953156}
{"id": "pubmed23n0032_482", "title": "Evolution of C4 photosynthesis in response to changes in carbon and oxygen concentrations in the atmosphere through time.", "content": "The hypothesis is presented that C4 photosynthesis has evolved at lease in part in response to reduced efficiency of C3 photosynthesis due to increased oxygen partial pressures in our recent atmosphere. Oxygen competition with carbon dioxide for the active site on ribulose diphosphate carboxylase was of little consequence when atmospheric levels of CO2 were high and those of O2 were low. However at the present low CO2 and high O2 partial pressures, adaptations for protecting the enzyme from oxygen have been selected.", "contents": "Evolution of C4 photosynthesis in response to changes in carbon and oxygen concentrations in the atmosphere through time. The hypothesis is presented that C4 photosynthesis has evolved at lease in part in response to reduced efficiency of C3 photosynthesis due to increased oxygen partial pressures in our recent atmosphere. Oxygen competition with carbon dioxide for the active site on ribulose diphosphate carboxylase was of little consequence when atmospheric levels of CO2 were high and those of O2 were low. However at the present low CO2 and high O2 partial pressures, adaptations for protecting the enzyme from oxygen have been selected.", "PMID": 953157}
{"id": "pubmed23n0032_483", "title": "Linkages in thermal copolymers of lysine.", "content": "The thermal copolymerization of lysine with other alpha-amino acids has been studied further. The identity of the second amino acid influences various properties of the polymer obtained, including the proportion of alpha and epsilon linkages of lysine. A review of linkages in proteinoids indicated alpha and beta linkages for aspartic acid, alpha and gamma linkages for glutamic acid, alpha and epsilon linkages for lysine, and alpha linkages for other amino acids. Thermal proteinoids are thus more complex in types of linkage than are proteins.", "contents": "Linkages in thermal copolymers of lysine. The thermal copolymerization of lysine with other alpha-amino acids has been studied further. The identity of the second amino acid influences various properties of the polymer obtained, including the proportion of alpha and epsilon linkages of lysine. A review of linkages in proteinoids indicated alpha and beta linkages for aspartic acid, alpha and gamma linkages for glutamic acid, alpha and epsilon linkages for lysine, and alpha linkages for other amino acids. Thermal proteinoids are thus more complex in types of linkage than are proteins.", "PMID": 953158}
{"id": "pubmed23n0032_484", "title": "A model for competing polymers leading to their spatial separation.", "content": "A model for a prebiotic polymer synthesis in a gradient of monomer is presented. In the absence of mutations the synthesis of the polymer proceeds in the region where the monomer concentration is the highest. However if a favorable mutation occurs, the latter accumulates in the high concentration zone and the initial polymer is restricted to a poorer monomer concentration region.", "contents": "A model for competing polymers leading to their spatial separation. A model for a prebiotic polymer synthesis in a gradient of monomer is presented. In the absence of mutations the synthesis of the polymer proceeds in the region where the monomer concentration is the highest. However if a favorable mutation occurs, the latter accumulates in the high concentration zone and the initial polymer is restricted to a poorer monomer concentration region.", "PMID": 953159}
{"id": "pubmed23n0032_485", "title": "Thermal polymerization of amino acids under various atmospheres or at low pressures.", "content": "The kinds and proportions of amino acids formed in two simulated prebiotic experiments or detected in hydrolyzed extracts of three extraterrestrial samples were found to polymerize thermally under various atmospheres or at low pressures. Yields, tested properties, and amino acid compositions of the polymers were not influenced by the type of enveloping atmosphere, including two simulated prebiotic atmospheres and five pure gases. However, polyamino acids prepared at low pressure (0.02, 10(-4) atm) were obtained in appreciably greater yield than those synthesized at 1 atm; amino acid composition was somewhat influenced by low pressure. The results indicate that polyamino acids could have been formed thermally under a variety of possible prebiotic atmospheres and on planetary bodies of low atmospheric pressure.", "contents": "Thermal polymerization of amino acids under various atmospheres or at low pressures. The kinds and proportions of amino acids formed in two simulated prebiotic experiments or detected in hydrolyzed extracts of three extraterrestrial samples were found to polymerize thermally under various atmospheres or at low pressures. Yields, tested properties, and amino acid compositions of the polymers were not influenced by the type of enveloping atmosphere, including two simulated prebiotic atmospheres and five pure gases. However, polyamino acids prepared at low pressure (0.02, 10(-4) atm) were obtained in appreciably greater yield than those synthesized at 1 atm; amino acid composition was somewhat influenced by low pressure. The results indicate that polyamino acids could have been formed thermally under a variety of possible prebiotic atmospheres and on planetary bodies of low atmospheric pressure.", "PMID": 953160}
{"id": "pubmed23n0032_486", "title": "Biological clocks: endogenous cycles synchronized by subtle geophysical rhythms.", "content": "Organismic sensitivity to atmospheric electromagnetic forces, together with usually asymmetrical phase-response systems, have enabled a simple unified explanation of the biological clocks and their timed rhythms. The clocks, endogenous cycles synchronized by subtle geophysical Zeitgeber, are always accurate. The rhythms, freely phase labile, autophase in \"constant conditions\" to generate a host of overt frequencies.", "contents": "Biological clocks: endogenous cycles synchronized by subtle geophysical rhythms. Organismic sensitivity to atmospheric electromagnetic forces, together with usually asymmetrical phase-response systems, have enabled a simple unified explanation of the biological clocks and their timed rhythms. The clocks, endogenous cycles synchronized by subtle geophysical Zeitgeber, are always accurate. The rhythms, freely phase labile, autophase in \"constant conditions\" to generate a host of overt frequencies.", "PMID": 953161}
{"id": "pubmed23n0032_487", "title": "Polycondensation of alpha-amino acids by pyrosulfuric acid.", "content": "The thermal polycondensation of amino acids common to protein is promoted at 80 degrees C by pyrosulfuric acid. This is in contrast to the noncondensation at 100 degrees C in the presence of concentrated sulfuric acid. These results are in accord with an anhydride mechanism, as proposed earlier for copolycondensation promoted by polyphosphoric acid. The amino acid composition, molecular weight, near-homogeneity, are infrared absorption of the polymer formed are described. The potential significance of planetary pyrosulfuric acid is discussed.", "contents": "Polycondensation of alpha-amino acids by pyrosulfuric acid. The thermal polycondensation of amino acids common to protein is promoted at 80 degrees C by pyrosulfuric acid. This is in contrast to the noncondensation at 100 degrees C in the presence of concentrated sulfuric acid. These results are in accord with an anhydride mechanism, as proposed earlier for copolycondensation promoted by polyphosphoric acid. The amino acid composition, molecular weight, near-homogeneity, are infrared absorption of the polymer formed are described. The potential significance of planetary pyrosulfuric acid is discussed.", "PMID": 953162}
{"id": "pubmed23n0032_488", "title": "The mode of interaction between the substrate and cell surface of the hydrocarbon-utilizing yeast Candida tropicalis.", "content": "A method for the measurement of the affinity of the cell surface to hydrocarbons is described. The affinity was basically unaffected by different pH values and temperature as well as by the chain length of the substrate. The contact time required for saturation of the cell surface with substrate was 30 sec. Cells grown on glucose showed a 25% lower adsorption capacity compared to those grown on n-alkane. The glucose grown cells showed also a more distinguished dependence of the amount of adsorbed hydrocarbon on the quality of the emulsion. The interaction between the substrate and cell surface turned out to be an adsorption that did not involve an enzymatic reaction. These results led to the conclusion that a lipopolysaccharide moiety present at the cell surface is responsible for the affinity.", "contents": "The mode of interaction between the substrate and cell surface of the hydrocarbon-utilizing yeast Candida tropicalis. A method for the measurement of the affinity of the cell surface to hydrocarbons is described. The affinity was basically unaffected by different pH values and temperature as well as by the chain length of the substrate. The contact time required for saturation of the cell surface with substrate was 30 sec. Cells grown on glucose showed a 25% lower adsorption capacity compared to those grown on n-alkane. The glucose grown cells showed also a more distinguished dependence of the amount of adsorbed hydrocarbon on the quality of the emulsion. The interaction between the substrate and cell surface turned out to be an adsorption that did not involve an enzymatic reaction. These results led to the conclusion that a lipopolysaccharide moiety present at the cell surface is responsible for the affinity.", "PMID": 953164}
{"id": "pubmed23n0032_489", "title": "Pore diffusion model for a two-substrate enzymatic reaction: application to galactose oxidase immobilized on porous glass particles.", "content": "An analysis of the pore diffusion model involving a two-substrate enzymatic reaction is presented. The resulting equations have been applied to the case of galactose oxidase catalyzed oxidation of galactose when the enzyme is immobilized on porous glass particles. The physical constants of the system were obtained by theoretical predictions and the enzyme concentration in the porous medium was derived from the experimental results. The calculations were performed with the assumption that the kinetic parameters of the enzyme remain unchanged upon immobilization. The theoretically calculated effectiveness factors were compared with the experimental effectiveness factors determined from the batch kinetic experiments and were found to be in agreement. The results are presented as effectiveness factor plots graphed as functions of bulk galactose and oxygen concentrations. The model was extended in order to study the effect of external mass transfer coefficients and pore enzyme concentrations on the effectiveness factors.", "contents": "Pore diffusion model for a two-substrate enzymatic reaction: application to galactose oxidase immobilized on porous glass particles. An analysis of the pore diffusion model involving a two-substrate enzymatic reaction is presented. The resulting equations have been applied to the case of galactose oxidase catalyzed oxidation of galactose when the enzyme is immobilized on porous glass particles. The physical constants of the system were obtained by theoretical predictions and the enzyme concentration in the porous medium was derived from the experimental results. The calculations were performed with the assumption that the kinetic parameters of the enzyme remain unchanged upon immobilization. The theoretically calculated effectiveness factors were compared with the experimental effectiveness factors determined from the batch kinetic experiments and were found to be in agreement. The results are presented as effectiveness factor plots graphed as functions of bulk galactose and oxygen concentrations. The model was extended in order to study the effect of external mass transfer coefficients and pore enzyme concentrations on the effectiveness factors.", "PMID": 953165}
{"id": "pubmed23n0032_490", "title": "Immobilization of a cephalosporin acetylesterase by containment within an ultrafiltration device.", "content": "A cephalosporin acetylesterase produced by Bacillus subtilis catalyzes the deacetylation of 7-aminocephalosporanic acid (7-ACA). Previous reports from our laboratory described the kinetic constants that characterize the reaction: Km = 2.8 X 10(-3)M, Kia acetate = 5 X 10(-2)M, and Kid deacetyl-7-ACA = 3.6 X 10(-2)M. These constants were used to predict the time course of the reaction using the following equation for dual competitive product inhibition. (see article) where St =mg/ml 7-ACA, At =mg/ml acetate, Dt =mg/ml deacetyl-7-ACA. The predicted time course closely matched the time course measured experimentally. The equation also was solved without the inhibition terms and the solution indicated that product inhibition caused about a 30% increase in the time required for complete (greater than 97%) hydrolysis of a 24 mg/ml 7-ACA solution. The esterase was immobilized by containment within an ultrafiltration device. With this technique the enzyme was reused 20 times over an 11 day span to deacetylate 7-ACA solutions containing 4 to 24 mg/ml 7-ACA. The specific activity after the 20th use was the same as the activity prior to the first use, indicating little enzyme inactiviation occurred.", "contents": "Immobilization of a cephalosporin acetylesterase by containment within an ultrafiltration device. A cephalosporin acetylesterase produced by Bacillus subtilis catalyzes the deacetylation of 7-aminocephalosporanic acid (7-ACA). Previous reports from our laboratory described the kinetic constants that characterize the reaction: Km = 2.8 X 10(-3)M, Kia acetate = 5 X 10(-2)M, and Kid deacetyl-7-ACA = 3.6 X 10(-2)M. These constants were used to predict the time course of the reaction using the following equation for dual competitive product inhibition. (see article) where St =mg/ml 7-ACA, At =mg/ml acetate, Dt =mg/ml deacetyl-7-ACA. The predicted time course closely matched the time course measured experimentally. The equation also was solved without the inhibition terms and the solution indicated that product inhibition caused about a 30% increase in the time required for complete (greater than 97%) hydrolysis of a 24 mg/ml 7-ACA solution. The esterase was immobilized by containment within an ultrafiltration device. With this technique the enzyme was reused 20 times over an 11 day span to deacetylate 7-ACA solutions containing 4 to 24 mg/ml 7-ACA. The specific activity after the 20th use was the same as the activity prior to the first use, indicating little enzyme inactiviation occurred.", "PMID": 953166}
{"id": "pubmed23n0032_491", "title": "A mathematical model for microbial growth under limitation by conservative substrates.", "content": "A mathematical model is suggested for growth of microorganisms under limitation by \"conservative\" substrates such as inorganic ions or vitamins that are not broken down after uptake into the cells, but that wholely or partly remain available for production of biomass. The specific growth rate is expressed here as a function of the intracellular \"concentration\" of the limiting substrate, defined as the amount of substrate within the cells per unit of cell dry weight. In the model, the intracellular substrate is divided into two parts. One part is a \"structural\" substrate not available for further growth. The other part is an \"excess\" or \"functional\" substrate that is used for biomass production and is assumed to be converted into structural substrate proportionally to growth. The rate of growth is believed to be controlled by the intracellular concentration of excess substrate.", "contents": "A mathematical model for microbial growth under limitation by conservative substrates. A mathematical model is suggested for growth of microorganisms under limitation by \"conservative\" substrates such as inorganic ions or vitamins that are not broken down after uptake into the cells, but that wholely or partly remain available for production of biomass. The specific growth rate is expressed here as a function of the intracellular \"concentration\" of the limiting substrate, defined as the amount of substrate within the cells per unit of cell dry weight. In the model, the intracellular substrate is divided into two parts. One part is a \"structural\" substrate not available for further growth. The other part is an \"excess\" or \"functional\" substrate that is used for biomass production and is assumed to be converted into structural substrate proportionally to growth. The rate of growth is believed to be controlled by the intracellular concentration of excess substrate.", "PMID": 953167}
{"id": "pubmed23n0032_492", "title": "Computer assistance in multiparameter flow microphotometry of mammalian cells.", "content": "Flow system technology enables the biological and medical experimenter to investigate the distribution spectra of various cellular characteristics separately or in parameter combination on the basis of ultrarapid single cell measurements. A typical rate of recognition is about 1000 to 5000 cells per second and the precision of measurements and their statistical relevance has been previously unobtainable. According to the approach of the multiparameter analysis and high data rate, computer assistance in flow system technology is given primary consideration. In this study three different kinds of software controlled modes in data acquisition are demonstrated: normal acquisition and linear accumulation of single parameters, spectra accumulation of two correlated parameters of each single cell and documentation as linear, two- or three-dimensional distribution pattern, and linear accumulation of two correlated parameters simultaneously with their actual signal-to-signal ratio. A first attempt to analyze distribution spectra was the application of the entropy of the structure routinely used in cybernetics. This function seems to be a measure for determining the degree of synchrony in an appropriate pretreated cell population. A special mathematical strategy has been applied to the linear spectra cellular DNA content, whose advantage is the quantitative extraction of the fractions concerning the various phases of the life cycle cells. The validity of this special curve fitting procedure has been proven on various experimental cell populations.", "contents": "Computer assistance in multiparameter flow microphotometry of mammalian cells. Flow system technology enables the biological and medical experimenter to investigate the distribution spectra of various cellular characteristics separately or in parameter combination on the basis of ultrarapid single cell measurements. A typical rate of recognition is about 1000 to 5000 cells per second and the precision of measurements and their statistical relevance has been previously unobtainable. According to the approach of the multiparameter analysis and high data rate, computer assistance in flow system technology is given primary consideration. In this study three different kinds of software controlled modes in data acquisition are demonstrated: normal acquisition and linear accumulation of single parameters, spectra accumulation of two correlated parameters of each single cell and documentation as linear, two- or three-dimensional distribution pattern, and linear accumulation of two correlated parameters simultaneously with their actual signal-to-signal ratio. A first attempt to analyze distribution spectra was the application of the entropy of the structure routinely used in cybernetics. This function seems to be a measure for determining the degree of synchrony in an appropriate pretreated cell population. A special mathematical strategy has been applied to the linear spectra cellular DNA content, whose advantage is the quantitative extraction of the fractions concerning the various phases of the life cycle cells. The validity of this special curve fitting procedure has been proven on various experimental cell populations.", "PMID": 953168}
{"id": "pubmed23n0032_493", "title": "Microbiological leaching of a chalcopyrite concentrate by Thiobacillus ferrooxidans.", "content": "The microbiological leaching of a chalcopyrite concentrate has been investigated using a pure strain of Thiobacillus ferrooxidans. The optimum leaching conditions regarding pH, temperature, and pulp density were found to be 2.3, 35 degrees C, and 22% respectively. The energy of activation was calculated to be 16.7 kcal/mol. During these experiments the maximum rate of copper dissolution was about 215 mg/liters/hr and the final copper concentration was as high as 55 g/liter. This latter value is in the range of copper concentrations which may be used for direct electrorecovery of copper. Jarosite formation was observed during the leaching of the chalcopyrite concentrate. When the leach residue was reground to expose new substrate surface, subsequent leaching resulted in copper extractions up to about 80%. On the basis of this experimental work, a flow sheet has been proposed for commercial scale biohydrometallurgical treatment of high-grade chalcopyrite materials.", "contents": "Microbiological leaching of a chalcopyrite concentrate by Thiobacillus ferrooxidans. The microbiological leaching of a chalcopyrite concentrate has been investigated using a pure strain of Thiobacillus ferrooxidans. The optimum leaching conditions regarding pH, temperature, and pulp density were found to be 2.3, 35 degrees C, and 22% respectively. The energy of activation was calculated to be 16.7 kcal/mol. During these experiments the maximum rate of copper dissolution was about 215 mg/liters/hr and the final copper concentration was as high as 55 g/liter. This latter value is in the range of copper concentrations which may be used for direct electrorecovery of copper. Jarosite formation was observed during the leaching of the chalcopyrite concentrate. When the leach residue was reground to expose new substrate surface, subsequent leaching resulted in copper extractions up to about 80%. On the basis of this experimental work, a flow sheet has been proposed for commercial scale biohydrometallurgical treatment of high-grade chalcopyrite materials.", "PMID": 953169}
{"id": "pubmed23n0032_494", "title": "Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures.", "content": "The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E/X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E/X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.", "contents": "Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures. The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E/X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E/X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.", "PMID": 953170}
{"id": "pubmed23n0032_495", "title": "Dual control of invertase biosynthesis in chemostat culture.", "content": "In a previous study on the chemostat culture of Saccharomyces carlsbergensis, maximum invertase specific activity was observed at an intermediate dilution rate. A possible regulation mechanism, assuming there are simultaneous effects of induction and repression on two sites of the operator loci for invertase formation, is proposed which might account for the observed curve of the dilution rate effect.", "contents": "Dual control of invertase biosynthesis in chemostat culture. In a previous study on the chemostat culture of Saccharomyces carlsbergensis, maximum invertase specific activity was observed at an intermediate dilution rate. A possible regulation mechanism, assuming there are simultaneous effects of induction and repression on two sites of the operator loci for invertase formation, is proposed which might account for the observed curve of the dilution rate effect.", "PMID": 953171}
{"id": "pubmed23n0032_496", "title": "Effect of broad and narrow spectrum fluorescent light on blood constituents.", "content": "Riboflavin in whole blood, G-6-PD activity in erythrocytes and amino acids in plasma of infants irradiated by broad and narrow spectrum blue fluorescent light were determined. Riboflavin was reduced by both types of light. Loss of G-6-PD activity during phototherapy with subsequent hemolysis was observed in some infants under each type of light source, both in vivo and in vitro. This phenomenon may be related to the indirect effect of riboflavin reduction and not to a direct effect upon the enzyme. The reduction of the plasma concentration of some amino acids observed under broad spectrum irradiation but not under pure blue light may not be of significance, but is of concern. These data indicate that all fluorescent light sources in current use for phototherapy produce biologic effects of importance beside the primary purpose of the treatment to reduce hyperbilirubinemia. It is apparent from the results of these studies that phototherapy light penetrates more deeply than the superficial skin to exert photochemical effects.", "contents": "Effect of broad and narrow spectrum fluorescent light on blood constituents. Riboflavin in whole blood, G-6-PD activity in erythrocytes and amino acids in plasma of infants irradiated by broad and narrow spectrum blue fluorescent light were determined. Riboflavin was reduced by both types of light. Loss of G-6-PD activity during phototherapy with subsequent hemolysis was observed in some infants under each type of light source, both in vivo and in vitro. This phenomenon may be related to the indirect effect of riboflavin reduction and not to a direct effect upon the enzyme. The reduction of the plasma concentration of some amino acids observed under broad spectrum irradiation but not under pure blue light may not be of significance, but is of concern. These data indicate that all fluorescent light sources in current use for phototherapy produce biologic effects of importance beside the primary purpose of the treatment to reduce hyperbilirubinemia. It is apparent from the results of these studies that phototherapy light penetrates more deeply than the superficial skin to exert photochemical effects.", "PMID": 953178}
{"id": "pubmed23n0032_497", "title": "Experience with Sephadex gel filtration in assessing the risk of bilirubin encephalopathy in neonatal jaundice.", "content": "Clinical experience with Sephadex gel filtration for evaluation of the bilirubin binding affinity of serum in neonatal jaundice is reported. In serum specimens from 362 jaundiced neonates the results of the test were in most cases in good agreement with the independently determined decision to perform exchange transfusion. In all relevant cases of the 24 with bilirubin encephalopathy the Sephadex test was strongly positive. The test was positive at lower bilirubin levels and bilirubin:albumin molar ratios in preterm as compared with term neonates, especially those in poor clinical conditions. Among term infants the test indicated an increase in bilirubin binding affinity after the fifth day of life. The Sephadex test appears to be of practical value in supplementing and refining existing criteria for exchange transfusion.", "contents": "Experience with Sephadex gel filtration in assessing the risk of bilirubin encephalopathy in neonatal jaundice. Clinical experience with Sephadex gel filtration for evaluation of the bilirubin binding affinity of serum in neonatal jaundice is reported. In serum specimens from 362 jaundiced neonates the results of the test were in most cases in good agreement with the independently determined decision to perform exchange transfusion. In all relevant cases of the 24 with bilirubin encephalopathy the Sephadex test was strongly positive. The test was positive at lower bilirubin levels and bilirubin:albumin molar ratios in preterm as compared with term neonates, especially those in poor clinical conditions. Among term infants the test indicated an increase in bilirubin binding affinity after the fifth day of life. The Sephadex test appears to be of practical value in supplementing and refining existing criteria for exchange transfusion.", "PMID": 953183}
{"id": "pubmed23n0032_498", "title": "Clinical experience with Sephadex gel filtration for the estimation of non-albumin bound bilirubin in sera of jaundiced infants.", "content": "Sephadex G-25 (Kernlute test) yields satisfactory results for the identification of infants at risk for BE and for estimation of the bilirubin binding capacity of the serum. In the presence of more than 2 mg/100 ml of conjugated bilirubin, the interpretation of the Sephadex G-25 test requires caution. Gel filtration through Sephadex LH-20 and chloroform elution of the free unconjugated bilirubin not bound to albumin seems to be a useful tool for eliminating interference by conjugated bilirubin, which is not chloroform-extractable.", "contents": "Clinical experience with Sephadex gel filtration for the estimation of non-albumin bound bilirubin in sera of jaundiced infants. Sephadex G-25 (Kernlute test) yields satisfactory results for the identification of infants at risk for BE and for estimation of the bilirubin binding capacity of the serum. In the presence of more than 2 mg/100 ml of conjugated bilirubin, the interpretation of the Sephadex G-25 test requires caution. Gel filtration through Sephadex LH-20 and chloroform elution of the free unconjugated bilirubin not bound to albumin seems to be a useful tool for eliminating interference by conjugated bilirubin, which is not chloroform-extractable.", "PMID": 953185}
{"id": "pubmed23n0032_499", "title": "Clinical application of phototherapy in neonatal jaundice.", "content": "Phototherapy is effective in preventing jaundice in the LBW infant. Using the schedule of lights on for 12 hours and off for 12 hours, continuous phototherapy was more effective than intermittent phototherapy. Metabolic requirements of the infant during phototherapy appear to increase. Whether these can be offset by appropriate therapy requires further study. No deleterious long-term effects of phototherapy were found on 2-year follow-up.", "contents": "Clinical application of phototherapy in neonatal jaundice. Phototherapy is effective in preventing jaundice in the LBW infant. Using the schedule of lights on for 12 hours and off for 12 hours, continuous phototherapy was more effective than intermittent phototherapy. Metabolic requirements of the infant during phototherapy appear to increase. Whether these can be offset by appropriate therapy requires further study. No deleterious long-term effects of phototherapy were found on 2-year follow-up.", "PMID": 953187}
{"id": "pubmed23n0032_500", "title": "Characteristics of a photoproduct of bilirubin found in vitro and in vivo, and its effect on the bilirubin binding affinity of serum.", "content": "The 430 pigment isolated from bilirubin-albumin solutions illuminated in vitro, decreased the bilirubin binding affinity of serum. This effect was demonstrated for both tightly and loosely bound bilirubin in serum, but in albumin solutions pretreated with charcoal only the binding of loosely bound bilirubin was decreased. This finding is of importance because a similar pigment has been isolated from the body fluids of an infant with Crigler-Najjar syndrome. The production and accumulation of 430 pigment during phototherapy might decrease the binding of bilirubin to albumin in the circulation.", "contents": "Characteristics of a photoproduct of bilirubin found in vitro and in vivo, and its effect on the bilirubin binding affinity of serum. The 430 pigment isolated from bilirubin-albumin solutions illuminated in vitro, decreased the bilirubin binding affinity of serum. This effect was demonstrated for both tightly and loosely bound bilirubin in serum, but in albumin solutions pretreated with charcoal only the binding of loosely bound bilirubin was decreased. This finding is of importance because a similar pigment has been isolated from the body fluids of an infant with Crigler-Najjar syndrome. The production and accumulation of 430 pigment during phototherapy might decrease the binding of bilirubin to albumin in the circulation.", "PMID": 953192}
{"id": "pubmed23n0032_501", "title": "Effect of phototherapy on bilirubin excretion in man and the rat.", "content": "Further evidence that the bilirubin excreted by Gunn rats during phototherapy is unconjugated is presented. The unconjugated bilirubin is not carried into bile as a water-soluble complex with rapidly excreted bilirubin derivatives, nor as a result of leakage of plasma protein into bile. Phototherapy does not augment biliary excretion of bilirubin in normal nonjaundiced rats, but does increase the concentration of conjugated bilirubin in the bile of patients with alcoholic cirrhosis, although their serum bilirubin concentrations are unaffected. The mechanism for augmented excretion of unconjugated bilirubin during phototherapy remains unexplained.", "contents": "Effect of phototherapy on bilirubin excretion in man and the rat. Further evidence that the bilirubin excreted by Gunn rats during phototherapy is unconjugated is presented. The unconjugated bilirubin is not carried into bile as a water-soluble complex with rapidly excreted bilirubin derivatives, nor as a result of leakage of plasma protein into bile. Phototherapy does not augment biliary excretion of bilirubin in normal nonjaundiced rats, but does increase the concentration of conjugated bilirubin in the bile of patients with alcoholic cirrhosis, although their serum bilirubin concentrations are unaffected. The mechanism for augmented excretion of unconjugated bilirubin during phototherapy remains unexplained.", "PMID": 953193}
{"id": "pubmed23n0032_502", "title": "Differential diagnosis and treatment of dislocated lenses.", "content": "Ectopia lentis continues to be a perplexing diagnostic and therapeutic problem for most ophthalmologists. However, thorough investigation, both systemic and ocular, can in most cases, establish either an environmental etiology or the nature of the heritable disorder. In cases with systemic disease and ectopia lentis, the ophthalmologist should insure that each patient obtains adequate consultation with other specialists in order that proper prophylactic and therapeutic measures are available. The Marfan syndrome, homocystinuria, and the Weill-Marchesani together account for the majority of dislocated lenses. Each of these requires a somewhat different therapeutic approach. Glaucoma should be recognized as a frequent complication in each of these conditions but is most serious in the Weill-Marchesani syndrome in which it often occurs early in life and remains unrecognized until serious damage has occurred. Because of the pupillary-block nature of the glaucoma in this condition, a prophylactic peripheral iridectomy must be seriously considered in every case while lens surgery should be undertaken if the glaucoma cannot be controlled by medical or surgical means. In homocystinuria, because of the potential vascular complications of general anesthesia, the ophthalmologist is often in a dilemma. The lens in this condition seems to be more mobile than in other ectopia lentis disorders and therefore likely to compromise vision earlier in life. However, due to the risk of vascular thrombosis, lens surgery should be delayed whenever possible until the patient is old enough to tolerate local anesthesia. Likewise, patients with the Marfan syndrome should be handled conservatively and, in general, function quite well simple with spectacle correction. Due to the high frequency of immediate surgical complications, it is advisable to avoid an open-sky technique to lens removal, using aspiration or a pars plana approach instead.", "contents": "Differential diagnosis and treatment of dislocated lenses. Ectopia lentis continues to be a perplexing diagnostic and therapeutic problem for most ophthalmologists. However, thorough investigation, both systemic and ocular, can in most cases, establish either an environmental etiology or the nature of the heritable disorder. In cases with systemic disease and ectopia lentis, the ophthalmologist should insure that each patient obtains adequate consultation with other specialists in order that proper prophylactic and therapeutic measures are available. The Marfan syndrome, homocystinuria, and the Weill-Marchesani together account for the majority of dislocated lenses. Each of these requires a somewhat different therapeutic approach. Glaucoma should be recognized as a frequent complication in each of these conditions but is most serious in the Weill-Marchesani syndrome in which it often occurs early in life and remains unrecognized until serious damage has occurred. Because of the pupillary-block nature of the glaucoma in this condition, a prophylactic peripheral iridectomy must be seriously considered in every case while lens surgery should be undertaken if the glaucoma cannot be controlled by medical or surgical means. In homocystinuria, because of the potential vascular complications of general anesthesia, the ophthalmologist is often in a dilemma. The lens in this condition seems to be more mobile than in other ectopia lentis disorders and therefore likely to compromise vision earlier in life. However, due to the risk of vascular thrombosis, lens surgery should be delayed whenever possible until the patient is old enough to tolerate local anesthesia. Likewise, patients with the Marfan syndrome should be handled conservatively and, in general, function quite well simple with spectacle correction. Due to the high frequency of immediate surgical complications, it is advisable to avoid an open-sky technique to lens removal, using aspiration or a pars plana approach instead.", "PMID": 953198}
{"id": "pubmed23n0032_503", "title": "Retinal involvement in a case of nephronophthisis associated with liver fibrosis Senior-Boichis syndrome.", "content": "Electroretinographic and electroencephalographic studies were conducted in a 12-year-old boy with nephronophthisis, chronic hepatic fibrosis, mental retardation and tapetoretinal degeneration (Senior-Boichis syndrome). Markedly reduced ERG amplitudes and flat oscillatory potentials were found in the proband. Delayed scotopic implicit time and reduced amplitudes of the beta-wave were found in the mother's ERG. ERG may identify the carrier state of  the Senior-Boichio syndrome.", "contents": "Retinal involvement in a case of nephronophthisis associated with liver fibrosis Senior-Boichis syndrome. Electroretinographic and electroencephalographic studies were conducted in a 12-year-old boy with nephronophthisis, chronic hepatic fibrosis, mental retardation and tapetoretinal degeneration (Senior-Boichis syndrome). Markedly reduced ERG amplitudes and flat oscillatory potentials were found in the proband. Delayed scotopic implicit time and reduced amplitudes of the beta-wave were found in the mother's ERG. ERG may identify the carrier state of  the Senior-Boichio syndrome.", "PMID": 953201}
{"id": "pubmed23n0032_504", "title": "Ring Y chromosome without mosaicism.", "content": "A 53-year-old man with a short stocky build, mild mental retardation, gynecomastia and hypogonadism was found to have a small ring Y chromosome unassociated with mosaicism. The ring Y was represented by a minute portion of chromatin or sometimes paired dots which were no larger than the short arm of the normally expected Y. No brightly fluorescent segment of the ring Y was present nor was it observed elsewhere in the karyotype. A primary medical problem was severe osteoarthritis, necessitating bilateral hip arthroplasties. Plasma testosterone was markedly decreased and plasma gonadotropins were increased. Potency was improved following testosterone injections. We conclude that genes responsible for testicular differentiation, maleness and possibly height are located close to the centromere of the Y chromosome, possibly on both the short and long arm. We also conclude that multiple genes are required for a fully developed male phenotype and apparently some of these genes were deleted or not expressed in this patient.", "contents": "Ring Y chromosome without mosaicism. A 53-year-old man with a short stocky build, mild mental retardation, gynecomastia and hypogonadism was found to have a small ring Y chromosome unassociated with mosaicism. The ring Y was represented by a minute portion of chromatin or sometimes paired dots which were no larger than the short arm of the normally expected Y. No brightly fluorescent segment of the ring Y was present nor was it observed elsewhere in the karyotype. A primary medical problem was severe osteoarthritis, necessitating bilateral hip arthroplasties. Plasma testosterone was markedly decreased and plasma gonadotropins were increased. Potency was improved following testosterone injections. We conclude that genes responsible for testicular differentiation, maleness and possibly height are located close to the centromere of the Y chromosome, possibly on both the short and long arm. We also conclude that multiple genes are required for a fully developed male phenotype and apparently some of these genes were deleted or not expressed in this patient.", "PMID": 953209}
{"id": "pubmed23n0032_505", "title": "Partial trisomy of chromosome 14: (+14q-).", "content": "Partial trisomy 14 as a 47, + 14q- karyotype is compatible with life and this state is distinguished on cytogenetic, not clinical, grounds. Clinical features are nonspecific and these children are most obvious because of growth and mental retardation. This extends and broadens the indications for chromosome analysis. The significance of finding a +14q- karyotype must, however, be considered in the knowledge that ESA fragments have been associated with disorders that do not have a chromosome aberration pathogenesis. A balanced translocation should always be sought in parents of affected children. Finally, the proximal long arm of chromosome 14 apparently has at least 2 sites of relative fragility: 14q12 in vitro and 14q22 in vivo. Blood group analyses have been uninformative (2, 5).", "contents": "Partial trisomy of chromosome 14: (+14q-). Partial trisomy 14 as a 47, + 14q- karyotype is compatible with life and this state is distinguished on cytogenetic, not clinical, grounds. Clinical features are nonspecific and these children are most obvious because of growth and mental retardation. This extends and broadens the indications for chromosome analysis. The significance of finding a +14q- karyotype must, however, be considered in the knowledge that ESA fragments have been associated with disorders that do not have a chromosome aberration pathogenesis. A balanced translocation should always be sought in parents of affected children. Finally, the proximal long arm of chromosome 14 apparently has at least 2 sites of relative fragility: 14q12 in vitro and 14q22 in vivo. Blood group analyses have been uninformative (2, 5).", "PMID": 953211}
{"id": "pubmed23n0032_506", "title": "Deletion of 11q: report of two cases and a review.", "content": "A terminal deletion of the long arm of chromosome 11 is compatible with life, and the affected child uniformly shows growth and mental retardation and telecanthus. However, no clear-cut, distinctive clinical syndrome is apparent. Mental and growth retardation alone may be the sole indications for chromosome analysis, as the virtual absence of dysmorphic features in some cases (Case 2) is noteworthy. Other appropriate family members should be evaluated to determine whether there is a familial balanced reciprocal translocation present and proper genetic counseling provided.", "contents": "Deletion of 11q: report of two cases and a review. A terminal deletion of the long arm of chromosome 11 is compatible with life, and the affected child uniformly shows growth and mental retardation and telecanthus. However, no clear-cut, distinctive clinical syndrome is apparent. Mental and growth retardation alone may be the sole indications for chromosome analysis, as the virtual absence of dysmorphic features in some cases (Case 2) is noteworthy. Other appropriate family members should be evaluated to determine whether there is a familial balanced reciprocal translocation present and proper genetic counseling provided.", "PMID": 953212}
{"id": "pubmed23n0032_507", "title": "Terminal (1)(q43) long-arm deletion of chromosome no. 1 in a three-year-old female.", "content": "A 3-year-old Latin female is reported with a terminal deletion of the No. 1 chromosome, karyotype formula 46, XX, del(1) (q43). Principle clinical features include: Anatomic - microcephaly; bilateral, convergent strabismus; epicanthus; brachycephaly; bulbar nose; sparse hair; partial soft tissue syndactylism between 2nd and 3rd fingers which are slightly tapered; whorls on all 10 fingers; mild prognathism; solitary kidney; vaginal stenosis; vesicoureteral reflux; asymmetric feet; and subluxation of peroneal tendons around the fibula with severe pronation and heal valgus deformity. Neurologic - moderate motor and mental retardation; high-pitched, shrill cry; absent pincer grasp at 3 years; and grand mal seizures documented from 9 months of age.", "contents": "Terminal (1)(q43) long-arm deletion of chromosome no. 1 in a three-year-old female. A 3-year-old Latin female is reported with a terminal deletion of the No. 1 chromosome, karyotype formula 46, XX, del(1) (q43). Principle clinical features include: Anatomic - microcephaly; bilateral, convergent strabismus; epicanthus; brachycephaly; bulbar nose; sparse hair; partial soft tissue syndactylism between 2nd and 3rd fingers which are slightly tapered; whorls on all 10 fingers; mild prognathism; solitary kidney; vaginal stenosis; vesicoureteral reflux; asymmetric feet; and subluxation of peroneal tendons around the fibula with severe pronation and heal valgus deformity. Neurologic - moderate motor and mental retardation; high-pitched, shrill cry; absent pincer grasp at 3 years; and grand mal seizures documented from 9 months of age.", "PMID": 953213}
{"id": "pubmed23n0032_508", "title": "Trisomy C and cystic dysplasia of kidneys, liver and pancreas.", "content": "Trisomy C is documented in a 17-hour-old full-term male infant with bilateral diffuse renal dysplasia, Potter facies, pulmonary hypoplasia and other congenital anomalies. In addition to renal dysplasia, intrahepatic bile duct and pancreatic dysplasia are demonstrated microscopically. The extra C-group autosome is morphologically consistent with a No. 11 and this is regarded as the etiology of the multiple malformations of this infant. The phenotype and the renal malformation are essentially similar to those described by Juberg et al (3) in an infant with trisomy C. It is suggested that the same chromosome is involved in both cases. Karyotype analysis should be performed on patients suspected of congenital renal malformations since the evidence implicates trisomy C as the etiology of at least some cases of bilateral renal dysplasia.", "contents": "Trisomy C and cystic dysplasia of kidneys, liver and pancreas. Trisomy C is documented in a 17-hour-old full-term male infant with bilateral diffuse renal dysplasia, Potter facies, pulmonary hypoplasia and other congenital anomalies. In addition to renal dysplasia, intrahepatic bile duct and pancreatic dysplasia are demonstrated microscopically. The extra C-group autosome is morphologically consistent with a No. 11 and this is regarded as the etiology of the multiple malformations of this infant. The phenotype and the renal malformation are essentially similar to those described by Juberg et al (3) in an infant with trisomy C. It is suggested that the same chromosome is involved in both cases. Karyotype analysis should be performed on patients suspected of congenital renal malformations since the evidence implicates trisomy C as the etiology of at least some cases of bilateral renal dysplasia.", "PMID": 953215}
{"id": "pubmed23n0032_509", "title": "A syndrome manifested by brittle hair with morphologic and biochemical abnormalities, developmental delay and normal stature.", "content": "We have presented 2 affected sibs-a male and female-with unaffected parents and sib from a small remote northern Mexican village. The syndrome includes mental deficit, brittle hair with decreased cuticular layer and an apparently collapsed cortex. The patients' hair contains decreased sulfur content and increased concentrations of trace elements as determined by x-ray fluorescent spectroscopy. Studies are underway to evaluate other apparently similarly affected children from the village where our family originated.", "contents": "A syndrome manifested by brittle hair with morphologic and biochemical abnormalities, developmental delay and normal stature. We have presented 2 affected sibs-a male and female-with unaffected parents and sib from a small remote northern Mexican village. The syndrome includes mental deficit, brittle hair with decreased cuticular layer and an apparently collapsed cortex. The patients' hair contains decreased sulfur content and increased concentrations of trace elements as determined by x-ray fluorescent spectroscopy. Studies are underway to evaluate other apparently similarly affected children from the village where our family originated.", "PMID": 953226}
{"id": "pubmed23n0032_510", "title": "Deafness and vitiligo.", "content": "Seven cases of congenital deafness and an autosomal recessive pedigree pattern were observed in an inbred kindred. Three of the cases also had vitiligo, and there were 2 other cases of vitiligo in this kindred. The vitiligo also appeared to have an autosomal recessive inheritance pattern.", "contents": "Deafness and vitiligo. Seven cases of congenital deafness and an autosomal recessive pedigree pattern were observed in an inbred kindred. Three of the cases also had vitiligo, and there were 2 other cases of vitiligo in this kindred. The vitiligo also appeared to have an autosomal recessive inheritance pattern.", "PMID": 953236}
{"id": "pubmed23n0032_511", "title": "Tumoral calcinosis and Engelmann disease.", "content": "Four cases of Engelmann disease were observed in a dominant inheritance pattern in a family of St. Landry Mulattoes. In the youngest sibship, one of the cases also had tumoral calcinosis. Another sib had only tumoral calcinosis, though some findings suggested that this sib may also have had Engelmann disease. All 3 of the younger sibs had snow-capped teeth. It is suggested by this family that snow-capped teeth may be part of the syndrome of Engelmann disease.", "contents": "Tumoral calcinosis and Engelmann disease. Four cases of Engelmann disease were observed in a dominant inheritance pattern in a family of St. Landry Mulattoes. In the youngest sibship, one of the cases also had tumoral calcinosis. Another sib had only tumoral calcinosis, though some findings suggested that this sib may also have had Engelmann disease. All 3 of the younger sibs had snow-capped teeth. It is suggested by this family that snow-capped teeth may be part of the syndrome of Engelmann disease.", "PMID": 953237}
{"id": "pubmed23n0032_512", "title": "Sacral agenesis and associated anomalies.", "content": "Sacral agenesis can no longer be considered an uncommon disorder. The condition occurs often enough that the clinician should be alert to any signs that may suggest its presence in a newborn. This disease is frequently associated with other anomalies, especially those of musculoskeletal, GU and GI systems. Diagnosis--early diagnosis--is the key to prevention of permanent damage to the kidneys and urinary tract in patients with the disease.", "contents": "Sacral agenesis and associated anomalies. Sacral agenesis can no longer be considered an uncommon disorder. The condition occurs often enough that the clinician should be alert to any signs that may suggest its presence in a newborn. This disease is frequently associated with other anomalies, especially those of musculoskeletal, GU and GI systems. Diagnosis--early diagnosis--is the key to prevention of permanent damage to the kidneys and urinary tract in patients with the disease.", "PMID": 953242}
{"id": "pubmed23n0032_513", "title": "Aberrant tissue bands and craniofacial defects.", "content": "A report of 8 cases of craniofacial anomalies and aberrant tissue bands has been presented. Analyses of the pedigrees, pregnancy histories and chromosome analyses were unrevealing. This syndrome appears to be a sporadic disorder and it is important to delineate it further from other syndromes for which recurrence risks are known.", "contents": "Aberrant tissue bands and craniofacial defects. A report of 8 cases of craniofacial anomalies and aberrant tissue bands has been presented. Analyses of the pedigrees, pregnancy histories and chromosome analyses were unrevealing. This syndrome appears to be a sporadic disorder and it is important to delineate it further from other syndromes for which recurrence risks are known.", "PMID": 953243}
{"id": "pubmed23n0032_514", "title": "E trisomy phenotype associated with small metacentric chromosome and a familial Y-22 translocation.", "content": "As a result of this case report, several entities are postulated due to an extra metacentric D or E chromosome: 1) infants presenting with a phenotype similar to the E 18 trisomy; however, the karyotype can be interpreted as either a deleted E or D chromosome; 2) another group of children all presenting with mental retardation, facial asymmetry, scoliosis and cerebral palsy, postulated due to a partial trisomy of E 16 or E 17; 3) individuals with a normal phenotype, but chromosomally presented with an additional satellited metacentric chromosome consistent with centric fusion of a D or G chromosome and 4) children presenting with an inconsistent phenotype and chromosomally presenting with an extra chromosome manifesting satellites or satellite association; the same chromosome abnormality often is found in unaffected parents and/or sibs.", "contents": "E trisomy phenotype associated with small metacentric chromosome and a familial Y-22 translocation. As a result of this case report, several entities are postulated due to an extra metacentric D or E chromosome: 1) infants presenting with a phenotype similar to the E 18 trisomy; however, the karyotype can be interpreted as either a deleted E or D chromosome; 2) another group of children all presenting with mental retardation, facial asymmetry, scoliosis and cerebral palsy, postulated due to a partial trisomy of E 16 or E 17; 3) individuals with a normal phenotype, but chromosomally presented with an additional satellited metacentric chromosome consistent with centric fusion of a D or G chromosome and 4) children presenting with an inconsistent phenotype and chromosomally presenting with an extra chromosome manifesting satellites or satellite association; the same chromosome abnormality often is found in unaffected parents and/or sibs.", "PMID": 953249}
{"id": "pubmed23n0032_515", "title": "[Effect of bradykinin on the microcirculatory bed of the rat small intestine mesentery].", "content": "The effect of application and of intravenous injection of bradykinin on the rat mesenteric microcirculation was studied by direct microscopy methods in vivo. Electrophysiological experiments failed to reveal the reaction of the vascular smooth muscle cells to bradykinin. Under the same conditions the dynamics of vasomotions was studied by the image-splitter method. It appeared that the microvascular response to bradykinin application varied and primarily depended on whether the application was primary or repeated. It is suggested that the action of bradykinin on the mesenteric vessels was mediated through the other vasoactive agents.", "contents": "[Effect of bradykinin on the microcirculatory bed of the rat small intestine mesentery]. The effect of application and of intravenous injection of bradykinin on the rat mesenteric microcirculation was studied by direct microscopy methods in vivo. Electrophysiological experiments failed to reveal the reaction of the vascular smooth muscle cells to bradykinin. Under the same conditions the dynamics of vasomotions was studied by the image-splitter method. It appeared that the microvascular response to bradykinin application varied and primarily depended on whether the application was primary or repeated. It is suggested that the action of bradykinin on the mesenteric vessels was mediated through the other vasoactive agents.", "PMID": 953258}
{"id": "pubmed23n0032_516", "title": "[Microcirculatory bed of the small intestine in different phases of its motor activity].", "content": "Experiments were conducted on guinea pigs, rabbits and rats with a simultaneous recording of the motor activity of the small intestine and the state on its microcirculatory bed, it was shown that the diameter of parietal vessels of the small intestine depended on the contractile activity of the muscles of this intestine. Proceeding from these data the authors explained the mechanism of derangement of the secretory and absorptive function of the small intestine after its neuro-reflex isolation and transplantation.", "contents": "[Microcirculatory bed of the small intestine in different phases of its motor activity]. Experiments were conducted on guinea pigs, rabbits and rats with a simultaneous recording of the motor activity of the small intestine and the state on its microcirculatory bed, it was shown that the diameter of parietal vessels of the small intestine depended on the contractile activity of the muscles of this intestine. Proceeding from these data the authors explained the mechanism of derangement of the secretory and absorptive function of the small intestine after its neuro-reflex isolation and transplantation.", "PMID": 953259}
{"id": "pubmed23n0032_517", "title": "[Effect of droperidol on corticofugal influences in food motivated responses].", "content": "The influences of droperidol (butyrophenon derivative) on feeding reaction evoked by electrical stimulation of the hypothalamic \"feeding centre\" were investigated in unrestricted rabbits. Intravenous injection of droperidol (0.3 mg/kg) decreased the threshold of evoked feeding. Inhibitory influences of the anterior areas of the brain cortex on feeding reactions were absent in the animals under droperidol, but the facilitating influences of the posterior areas of the brain cortex on feeding reactions were retained or even became stronger. These findings are explained by dophamine-negative action of droperidol and by disturbances of the inhibitory system of the brain.", "contents": "[Effect of droperidol on corticofugal influences in food motivated responses]. The influences of droperidol (butyrophenon derivative) on feeding reaction evoked by electrical stimulation of the hypothalamic \"feeding centre\" were investigated in unrestricted rabbits. Intravenous injection of droperidol (0.3 mg/kg) decreased the threshold of evoked feeding. Inhibitory influences of the anterior areas of the brain cortex on feeding reactions were absent in the animals under droperidol, but the facilitating influences of the posterior areas of the brain cortex on feeding reactions were retained or even became stronger. These findings are explained by dophamine-negative action of droperidol and by disturbances of the inhibitory system of the brain.", "PMID": 953260}
{"id": "pubmed23n0032_518", "title": "[Growth and hematopoiesis during early postnatal ontogenesis in rats subjected to cold exposure].", "content": "Rats developing the first month after birth under conditions of periodic cold exposures displayed earlier establishment of erythropoiesis as compared with control animals. This was expressed in a shorter period of physiological anemia and in increase of blood volume. As a result of improvement of body and tissue blood supply there occurred an acceleration of growth and development of experimental animals together with economy of energy expenditure at rest in comparison with control ratlings of the same age.", "contents": "[Growth and hematopoiesis during early postnatal ontogenesis in rats subjected to cold exposure]. Rats developing the first month after birth under conditions of periodic cold exposures displayed earlier establishment of erythropoiesis as compared with control animals. This was expressed in a shorter period of physiological anemia and in increase of blood volume. As a result of improvement of body and tissue blood supply there occurred an acceleration of growth and development of experimental animals together with economy of energy expenditure at rest in comparison with control ratlings of the same age.", "PMID": 953261}
{"id": "pubmed23n0032_519", "title": "[Changes in hemorheologic indices during hemorrhagic shock].", "content": "Experiments were conducted on dogs. A study was made of interrelationship between the rheological, hemodynamic and metabolic indices in hemorrhagic shock. As found, mechanical properties of the blood altered after the blood loss in parallel with the macrohemodynamic disturbances and preceded metabolic acidosis. A conclusion was drawn that determination of blood fluidity threshold could be applied for the establishment of the extent of hemodynamic disturbance in shock.", "contents": "[Changes in hemorheologic indices during hemorrhagic shock]. Experiments were conducted on dogs. A study was made of interrelationship between the rheological, hemodynamic and metabolic indices in hemorrhagic shock. As found, mechanical properties of the blood altered after the blood loss in parallel with the macrohemodynamic disturbances and preceded metabolic acidosis. A conclusion was drawn that determination of blood fluidity threshold could be applied for the establishment of the extent of hemodynamic disturbance in shock.", "PMID": 953262}
{"id": "pubmed23n0032_520", "title": "[Influence of burned skin toxin on the state of hemodynamics in rats].", "content": "The toxin of the burned skin of rats purified by the immunochemical method possessed a definite physiological activity towards the cardiovascular system of homologous animals. The preparation introduced into the circulation caused in the course of 2 hours a reduction of the total arterial pressure from 125 +/- 7 mm Hg 74 +/- 8 mm Hg. Development of systemic hypotension was accompanied by secondary disturbance of microcirculation in the mesentery of the small intestine in the form of contraction, of arterioles, retarding the blood flow along the venules, and reduction of the capillary circulation.", "contents": "[Influence of burned skin toxin on the state of hemodynamics in rats]. The toxin of the burned skin of rats purified by the immunochemical method possessed a definite physiological activity towards the cardiovascular system of homologous animals. The preparation introduced into the circulation caused in the course of 2 hours a reduction of the total arterial pressure from 125 +/- 7 mm Hg 74 +/- 8 mm Hg. Development of systemic hypotension was accompanied by secondary disturbance of microcirculation in the mesentery of the small intestine in the form of contraction, of arterioles, retarding the blood flow along the venules, and reduction of the capillary circulation.", "PMID": 953263}
{"id": "pubmed23n0032_521", "title": "[Changes in the mesenteric microcirculation of rats in acute hypoxic hypoxia].", "content": "Hypoxia was induced in rats giving them to inspire gaseous mixture corresponding to an altitude of 6000 m. Arterial and venous vessels of the mesentery, from 9 to 43 mum in diameter, were studied; their diameter, blood pressure and blood flow velocity in them were measured. Arterial microvessels proved to dilate in hypoxia. The maximal increase in their diameter (by 3-5 mum) occurred during the first 3-5 min after the gaseous mixture was given to inspire. Blood pressure and blood flow velocity in these vessels decreased during the whole course of the experiment.", "contents": "[Changes in the mesenteric microcirculation of rats in acute hypoxic hypoxia]. Hypoxia was induced in rats giving them to inspire gaseous mixture corresponding to an altitude of 6000 m. Arterial and venous vessels of the mesentery, from 9 to 43 mum in diameter, were studied; their diameter, blood pressure and blood flow velocity in them were measured. Arterial microvessels proved to dilate in hypoxia. The maximal increase in their diameter (by 3-5 mum) occurred during the first 3-5 min after the gaseous mixture was given to inspire. Blood pressure and blood flow velocity in these vessels decreased during the whole course of the experiment.", "PMID": 953264}
{"id": "pubmed23n0032_522", "title": "[Influence of prolonged hypokinesia on the water and fat composition of the human body].", "content": "A study was made of a combined action of prolonged hypokinesia (period - up to 30-49 days) and of different position of persons under trial (healthy men) to the gravitation vector on the body composition. Changes in total water content in the organism largely depended not on the limited mobility, but on the body position in space. In antiorthostatic position of persons under trial water \"elimination\" occurred chiefly during the first several days. The \"lean mass of the body\" decreased as a result of prolonged hypokinesia, and water content fell in it; but water content was rapidly restored with the restoration of the motor activity.", "contents": "[Influence of prolonged hypokinesia on the water and fat composition of the human body]. A study was made of a combined action of prolonged hypokinesia (period - up to 30-49 days) and of different position of persons under trial (healthy men) to the gravitation vector on the body composition. Changes in total water content in the organism largely depended not on the limited mobility, but on the body position in space. In antiorthostatic position of persons under trial water \"elimination\" occurred chiefly during the first several days. The \"lean mass of the body\" decreased as a result of prolonged hypokinesia, and water content fell in it; but water content was rapidly restored with the restoration of the motor activity.", "PMID": 953265}
{"id": "pubmed23n0032_523", "title": "[Resistance of regenerating liver to hepatotoxins].", "content": "Treatment of mice with CCl4 and paracetamol induced massive centrilobular necroses. Preliminary induction of hepatocyte proliferation by partial hepatectomy significantly weakened or completely eliminated the toxic action on the liver depending on the time passed after the operation. Practically no damage was observed two days after the operation. Damage of the liver with CCl4 paracetamol and other hepatotoxins is thought to be related to metabolism of these drugs by the hepatocytes and their transformation into toxic substances. In the regenerating liver a reconstruction of metabolism takes place which provides for the proliferative processes. Some tissue-specific functions of hepatocytes, drug metabolizing function in particular, are temporarily lost. This is responsible for nonsensibility of the regenerating liver to the toxins under study.", "contents": "[Resistance of regenerating liver to hepatotoxins]. Treatment of mice with CCl4 and paracetamol induced massive centrilobular necroses. Preliminary induction of hepatocyte proliferation by partial hepatectomy significantly weakened or completely eliminated the toxic action on the liver depending on the time passed after the operation. Practically no damage was observed two days after the operation. Damage of the liver with CCl4 paracetamol and other hepatotoxins is thought to be related to metabolism of these drugs by the hepatocytes and their transformation into toxic substances. In the regenerating liver a reconstruction of metabolism takes place which provides for the proliferative processes. Some tissue-specific functions of hepatocytes, drug metabolizing function in particular, are temporarily lost. This is responsible for nonsensibility of the regenerating liver to the toxins under study.", "PMID": 953266}
{"id": "pubmed23n0032_524", "title": "[Artificial \"adaptation\" to cold using noradrenaline].", "content": "A study was undertaken to evaluate the muscle thermogenesis of rabbits 2-3 weeks after the intravenous injection of norepinephrine under conditions of cold. Electrical activity of the muscles proved to decrease single administration of norepinephrine proved to maintain (for several weeks) the temperature homeostasis with a much lesser electrical activity of the muscles than before the administration of norepinephrine. Apparently this phenomenon underlied adaptation of animals to cold.", "contents": "[Artificial \"adaptation\" to cold using noradrenaline]. A study was undertaken to evaluate the muscle thermogenesis of rabbits 2-3 weeks after the intravenous injection of norepinephrine under conditions of cold. Electrical activity of the muscles proved to decrease single administration of norepinephrine proved to maintain (for several weeks) the temperature homeostasis with a much lesser electrical activity of the muscles than before the administration of norepinephrine. Apparently this phenomenon underlied adaptation of animals to cold.", "PMID": 953268}
{"id": "pubmed23n0032_525", "title": "[Different heparin fractions].", "content": "It was shown that treatment by 2-3 M KCl solutions of complex unfractionated preparations of heparin with hexamminecobalt (III) resulted in incorporation into the solution of a heparin fraction containing 3 residues of sulphuric acid per 1 residue in glucosamine. In the precipitate released of hexamminecobalt (III) there remains a heparin fraction containing 4 residues of sulphuric acid per 1 residue of glucosamine. Both fractions were isolated in a chemically individual form.", "contents": "[Different heparin fractions]. It was shown that treatment by 2-3 M KCl solutions of complex unfractionated preparations of heparin with hexamminecobalt (III) resulted in incorporation into the solution of a heparin fraction containing 3 residues of sulphuric acid per 1 residue in glucosamine. In the precipitate released of hexamminecobalt (III) there remains a heparin fraction containing 4 residues of sulphuric acid per 1 residue of glucosamine. Both fractions were isolated in a chemically individual form.", "PMID": 953269}
{"id": "pubmed23n0032_526", "title": "[Intensity of phospholipid and cholesterol synthesis in the brain and spinal cord of rabbits with experimental allergic encephelomyelitis].", "content": "Experimental allergic encephalomyelitis (EAE) was induced in rabbits by inoculation of homologous spinal cord myelin with Freund's adjuvant. During the paralytic stage of EAE rabbits were injected with 2-C14-acetate and sacrificed in two hours. As revealed, the intensity of phospholipid and cholesterol synthesis decreased markedly in rabbits with EAE not only in the lumbar part of the spinal cord (where the greatest lesions of myelin were localized), but also in the brain stem (where demyelinization was absent).", "contents": "[Intensity of phospholipid and cholesterol synthesis in the brain and spinal cord of rabbits with experimental allergic encephelomyelitis]. Experimental allergic encephalomyelitis (EAE) was induced in rabbits by inoculation of homologous spinal cord myelin with Freund's adjuvant. During the paralytic stage of EAE rabbits were injected with 2-C14-acetate and sacrificed in two hours. As revealed, the intensity of phospholipid and cholesterol synthesis decreased markedly in rabbits with EAE not only in the lumbar part of the spinal cord (where the greatest lesions of myelin were localized), but also in the brain stem (where demyelinization was absent).", "PMID": 953270}
{"id": "pubmed23n0032_527", "title": "[Intracellular distribution of hyaluronidase in rats with acute and chronic hepatitis and reparative liver regeneration].", "content": "Subcellular distribution of hyaluronidase of rat liver was studied during spontaneous restoration during the 1st, 2nd, 3rd days, and 1, 2, 3, 4 weeks of acute and chronic CCl4-hepatitis. Redistribution of relative specific activity of hyaluronidase was seen in the fractions of heavy and light mitochondria, microsomes and supernatant fraction, after an acute hepatic injury. Four weeks after the injury subcellular distribution was not yet normal. In chronic hepatitis the relative specific hyaluronidase activity was almost identical in the five subcellular fractions. In the course of restoration the relative specific activity was increased in the fractions of heavy and light mitochondria and microsomes, but decreased in the nuclear and supernatant fractions.", "contents": "[Intracellular distribution of hyaluronidase in rats with acute and chronic hepatitis and reparative liver regeneration]. Subcellular distribution of hyaluronidase of rat liver was studied during spontaneous restoration during the 1st, 2nd, 3rd days, and 1, 2, 3, 4 weeks of acute and chronic CCl4-hepatitis. Redistribution of relative specific activity of hyaluronidase was seen in the fractions of heavy and light mitochondria, microsomes and supernatant fraction, after an acute hepatic injury. Four weeks after the injury subcellular distribution was not yet normal. In chronic hepatitis the relative specific hyaluronidase activity was almost identical in the five subcellular fractions. In the course of restoration the relative specific activity was increased in the fractions of heavy and light mitochondria and microsomes, but decreased in the nuclear and supernatant fractions.", "PMID": 953271}
{"id": "pubmed23n0032_528", "title": "[Changes in the concentration of sialic acids in the intestinal tissues of animals immunized with live vaccine against poliomyelitis].", "content": "A single oral administration to albino mice weighing from 12 to 14 g of live poliomyelitis vaccine of the immunological type II was accompanied by an increase in the content of free sialic acids from the 5th to the 20th day and of bound sialic acids from the 20th to the 45th day from the beginning of the immunization, in the small intestine of the vaccinated animals.", "contents": "[Changes in the concentration of sialic acids in the intestinal tissues of animals immunized with live vaccine against poliomyelitis]. A single oral administration to albino mice weighing from 12 to 14 g of live poliomyelitis vaccine of the immunological type II was accompanied by an increase in the content of free sialic acids from the 5th to the 20th day and of bound sialic acids from the 20th to the 45th day from the beginning of the immunization, in the small intestine of the vaccinated animals.", "PMID": 953272}
{"id": "pubmed23n0032_529", "title": "[Several biochemical characteristics of rat skeletal musculature during the development under conditions of skeletal muscular loading and hypodynamia].", "content": "The weight and biochemistry of the skeletal muscles of rats developing under conditions of the action of the skeletomuscular loads (the I group), under conditions of hypodynamia - at the environmental temperature of 20-22 degrees C (the II group) and at the environmental temperature of 28-30 degrees C (the III group) were studied. Rats of the I group displayed an increase of the mass of the skeletal muscles and also an elevation of protein, glycogen, ATP, creatine phosphate and a decrease of lactic acid content in these muscles. Rats of the III group showed a considerable decrease in the content of enumerated indices both in comparison with indices of control animals and of rats of the I group. This decrease was less marked in rats of the II group.", "contents": "[Several biochemical characteristics of rat skeletal musculature during the development under conditions of skeletal muscular loading and hypodynamia]. The weight and biochemistry of the skeletal muscles of rats developing under conditions of the action of the skeletomuscular loads (the I group), under conditions of hypodynamia - at the environmental temperature of 20-22 degrees C (the II group) and at the environmental temperature of 28-30 degrees C (the III group) were studied. Rats of the I group displayed an increase of the mass of the skeletal muscles and also an elevation of protein, glycogen, ATP, creatine phosphate and a decrease of lactic acid content in these muscles. Rats of the III group showed a considerable decrease in the content of enumerated indices both in comparison with indices of control animals and of rats of the I group. This decrease was less marked in rats of the II group.", "PMID": 953274}
{"id": "pubmed23n0032_530", "title": "[Comparative characteristics of ribonuclease isolated from the lliver and pancreas of burned and intact rats].", "content": "Alkaline RNA-ase was isolated from the pancreas and liver of intact and burnt rats, and purified 1400 and 2000 times accordingly. The optimum of the enzyme activity was achieved at pH 7.6-7.8. Normal and post-burn RNA-ase biosynthesis was studied using radioamino acids (C14-lysine and C14-glycine). Burnt animals displayed a slowed down alkaline RNA-ase biosynthesis, both in the liver and in the pancreas. Ribonuclease \"half-life periods\" of the liver of intact rats was 65 min, whereas in that of the burnt animals it was prolonged to 100.7 min. The end-preparation activity of the liver and pancreas alkaline RNA-ase of the intact and burnt animals showed no significant difference.", "contents": "[Comparative characteristics of ribonuclease isolated from the lliver and pancreas of burned and intact rats]. Alkaline RNA-ase was isolated from the pancreas and liver of intact and burnt rats, and purified 1400 and 2000 times accordingly. The optimum of the enzyme activity was achieved at pH 7.6-7.8. Normal and post-burn RNA-ase biosynthesis was studied using radioamino acids (C14-lysine and C14-glycine). Burnt animals displayed a slowed down alkaline RNA-ase biosynthesis, both in the liver and in the pancreas. Ribonuclease \"half-life periods\" of the liver of intact rats was 65 min, whereas in that of the burnt animals it was prolonged to 100.7 min. The end-preparation activity of the liver and pancreas alkaline RNA-ase of the intact and burnt animals showed no significant difference.", "PMID": 953275}
{"id": "pubmed23n0032_531", "title": "[Stability of physico-chemical parameters of DNA during long-term storage].", "content": "DNA was extracted from calf thymus and sturgeon milt and purified of admixtures; this highly polymeric DNA was studied under conditions of its prolonged storage (for 1 1/2-2 years) at a temperature of 0-4 degrees C in 1 M NaCl in the form of a precipitate under a 70% ethanol and in the lyophilized state. It appeared that DNA obtained from the mentioned sources could be stored for at least 1 1/2 years under the said conditions without any significant damage of its polymeric properties or secondary structure. Although DNA from sturgeon milt had the initial physico-chemical indices similar to those of the thymus DNA, the former displayed much greater stability under the same storage condition, indicating stability of its molecular structure.", "contents": "[Stability of physico-chemical parameters of DNA during long-term storage]. DNA was extracted from calf thymus and sturgeon milt and purified of admixtures; this highly polymeric DNA was studied under conditions of its prolonged storage (for 1 1/2-2 years) at a temperature of 0-4 degrees C in 1 M NaCl in the form of a precipitate under a 70% ethanol and in the lyophilized state. It appeared that DNA obtained from the mentioned sources could be stored for at least 1 1/2 years under the said conditions without any significant damage of its polymeric properties or secondary structure. Although DNA from sturgeon milt had the initial physico-chemical indices similar to those of the thymus DNA, the former displayed much greater stability under the same storage condition, indicating stability of its molecular structure.", "PMID": 953276}
{"id": "pubmed23n0032_532", "title": "[Effect of retabolil on renal tubular secretion].", "content": "Chronic experiments on dogs and rats showed retabolil, an anabolic steroid (given in a dose of 2-5 mg/kg for 7 days) to produce an increase in renal tubular secretion of cardiotrast. Rats displayed a simultaneous elevation in the content of protein and RNA in the kidney cortex. The data obtained were interpreted from the aspect of protein nature of carriers taking part in the renal secretory transport.", "contents": "[Effect of retabolil on renal tubular secretion]. Chronic experiments on dogs and rats showed retabolil, an anabolic steroid (given in a dose of 2-5 mg/kg for 7 days) to produce an increase in renal tubular secretion of cardiotrast. Rats displayed a simultaneous elevation in the content of protein and RNA in the kidney cortex. The data obtained were interpreted from the aspect of protein nature of carriers taking part in the renal secretory transport.", "PMID": 953277}
{"id": "pubmed23n0032_533", "title": "[Effect of striatectomy on the course of corazole seizures in rats].", "content": "Behaviouristic and EEG-manifestations of pentylentetrazol convulsions were studied in unrestrained rats with preliminarily destroyed striatum. The operation caused no significant disturbance of the appearance of the spike-wave activity, but sharply inhibited provocation of myoclonic and their change into tonico-clonic convulsions. Besides, there was an increase in the threshold, the duration and the severity of the attack; an epileptic status was even observed in 50% of these rats. Striatectomy eliminated the action of catecholaminergic agents (apomorphine, DOPA, haloperidol, chlorpromazine) on the thresholds of myclonic jerks and the attack.", "contents": "[Effect of striatectomy on the course of corazole seizures in rats]. Behaviouristic and EEG-manifestations of pentylentetrazol convulsions were studied in unrestrained rats with preliminarily destroyed striatum. The operation caused no significant disturbance of the appearance of the spike-wave activity, but sharply inhibited provocation of myoclonic and their change into tonico-clonic convulsions. Besides, there was an increase in the threshold, the duration and the severity of the attack; an epileptic status was even observed in 50% of these rats. Striatectomy eliminated the action of catecholaminergic agents (apomorphine, DOPA, haloperidol, chlorpromazine) on the thresholds of myclonic jerks and the attack.", "PMID": 953278}
{"id": "pubmed23n0032_534", "title": "[Mechanism of action of nonachlazin on nervous regulation of coronary circulation].", "content": "In experiments on cats and dogs under general anesthesia nonachlazine, a new antianginal agent, depressed the reflex decrease of the blood flow to the coronary arteries (electromagnetic recording). Nonachlazine also depressed the reflex decrease of the blood flow in the system of common carotic artery, and the pressure vasomotor reflexes. During recording the tonic and reflex activity in the heart sympathetic nerves nonachlazine proved to selectively inhibit the vasoconstrictor impulses from Adelta-afferent fibers of the spinal nerves, that is it influenced the vasomotor component of the \"primary\" pain reaction. This mechanism could underlie the block by nonachlazine of the pain syndrome during the heart ischemia disease.", "contents": "[Mechanism of action of nonachlazin on nervous regulation of coronary circulation]. In experiments on cats and dogs under general anesthesia nonachlazine, a new antianginal agent, depressed the reflex decrease of the blood flow to the coronary arteries (electromagnetic recording). Nonachlazine also depressed the reflex decrease of the blood flow in the system of common carotic artery, and the pressure vasomotor reflexes. During recording the tonic and reflex activity in the heart sympathetic nerves nonachlazine proved to selectively inhibit the vasoconstrictor impulses from Adelta-afferent fibers of the spinal nerves, that is it influenced the vasomotor component of the \"primary\" pain reaction. This mechanism could underlie the block by nonachlazine of the pain syndrome during the heart ischemia disease.", "PMID": 953279}
{"id": "pubmed23n0032_535", "title": "[Isolation and immunochemical analysis of the breakdown products of gammaG-globulin contained in the serum of partially hepatectomized rabbits].", "content": "As demonstrated by immunochemical methods, the serum of partially hepatectomized rabbits contained a Fab-like fragment of IgG. This fragment was isolated by fractionation of the serum on CM-cellulose following affinity chromatography on Sepharose coated with antibodies against rabbit IgG. The sedimentation constant of the Fab-like fragment was 5.2 S; complete antigenic identity to pepsin F (ab')2 fragment was thus demonstrated. No Fab-like fragment was shown in the serum of sham-operated animals, or in the serum of partially hepatectomized rabbits injected with protease inhibitor Trasilol before and after the operation. It was concluded that Fab-like fragment found in the partially hepatectomized rabbits appeared as a result of splitting the autologous IgG with protease(s) from cells of the liver injured during the operation.", "contents": "[Isolation and immunochemical analysis of the breakdown products of gammaG-globulin contained in the serum of partially hepatectomized rabbits]. As demonstrated by immunochemical methods, the serum of partially hepatectomized rabbits contained a Fab-like fragment of IgG. This fragment was isolated by fractionation of the serum on CM-cellulose following affinity chromatography on Sepharose coated with antibodies against rabbit IgG. The sedimentation constant of the Fab-like fragment was 5.2 S; complete antigenic identity to pepsin F (ab')2 fragment was thus demonstrated. No Fab-like fragment was shown in the serum of sham-operated animals, or in the serum of partially hepatectomized rabbits injected with protease inhibitor Trasilol before and after the operation. It was concluded that Fab-like fragment found in the partially hepatectomized rabbits appeared as a result of splitting the autologous IgG with protease(s) from cells of the liver injured during the operation.", "PMID": 953281}
{"id": "pubmed23n0032_536", "title": "[Several properties of low-molecular weight tetanus antitoxin].", "content": "Following intravenous injection of tetanus antitoxin, obtained by tryptic digestion of the horse immunoglobulin \"Diaferm-3\", purification and concentration of active fragments, the antitoxin was eliminated from the rabbit organism three times more rapidly than after the injection of the original \"Diaferm-3\" antitoxin. After injection of the split antitoxin its urinary excretion lasted up to 6 days, whereas following injection of the \"Diaferm-3\" antitoxin it was excreted for up to 19 days; in the first case considerably less antitoxin was excreted than in the second one (2 and 3.5%, respectively). In both cases in the antitoxin excreted with urine represented monovalent. Fab'-fragments, producing a delay in precipitation in the cross reaction in agar gel between the tetanus toxoid and the tetanus antiserum. Fab'-fragment obtained by the mentioned method possessed anaphylactogenic properties.", "contents": "[Several properties of low-molecular weight tetanus antitoxin]. Following intravenous injection of tetanus antitoxin, obtained by tryptic digestion of the horse immunoglobulin \"Diaferm-3\", purification and concentration of active fragments, the antitoxin was eliminated from the rabbit organism three times more rapidly than after the injection of the original \"Diaferm-3\" antitoxin. After injection of the split antitoxin its urinary excretion lasted up to 6 days, whereas following injection of the \"Diaferm-3\" antitoxin it was excreted for up to 19 days; in the first case considerably less antitoxin was excreted than in the second one (2 and 3.5%, respectively). In both cases in the antitoxin excreted with urine represented monovalent. Fab'-fragments, producing a delay in precipitation in the cross reaction in agar gel between the tetanus toxoid and the tetanus antiserum. Fab'-fragment obtained by the mentioned method possessed anaphylactogenic properties.", "PMID": 953282}
{"id": "pubmed23n0032_537", "title": "[Brucellosis protective antigen, its chemical and immunobiological characteristics].", "content": "The data on brucella nontoxic protective antigen without any sensitizing properties isolated from the cell wall of brucella are presented. The antigen had a marked protective effect against the experimental infection for 3-4 months after a single immunization of guinea pigs.Molecular heterogeneity of the antigen, which is mainly a protein-polysaccharide complex, is given. High- and low-molecular fractions detected by gel filtration technique differed according to the chemical and immunobiological characteristics. The high-molecular fraction of the antigen was found to be highly immunogenic and serologically active.", "contents": "[Brucellosis protective antigen, its chemical and immunobiological characteristics]. The data on brucella nontoxic protective antigen without any sensitizing properties isolated from the cell wall of brucella are presented. The antigen had a marked protective effect against the experimental infection for 3-4 months after a single immunization of guinea pigs.Molecular heterogeneity of the antigen, which is mainly a protein-polysaccharide complex, is given. High- and low-molecular fractions detected by gel filtration technique differed according to the chemical and immunobiological characteristics. The high-molecular fraction of the antigen was found to be highly immunogenic and serologically active.", "PMID": 953283}
{"id": "pubmed23n0032_538", "title": "[Role of macrophages in the mechanism of specific entrapment of sensitized lymphocytes in lymph nodes containing antigen].", "content": "A study was made of the participation of macrophages in the phenomenon of specific trapping of lymphocytes activated with the antigen in the lymph nodes containing the same antigen. Administration of peritoneal macrophages which phagocytized in vitro sheep erythrocytes (as well as of erythrocytes alone) led to specific trapping of lymphocytes in the regional lymph nodes. Preliminary treatment of macrophages loaded with the antigen with antierythrocytic serum decreased the lymphocyte trapping. A supposition was put forward that lymphocytes with receptors on their surface were bound on the macrophage membrane into which the antigen molecules were incorporated.", "contents": "[Role of macrophages in the mechanism of specific entrapment of sensitized lymphocytes in lymph nodes containing antigen]. A study was made of the participation of macrophages in the phenomenon of specific trapping of lymphocytes activated with the antigen in the lymph nodes containing the same antigen. Administration of peritoneal macrophages which phagocytized in vitro sheep erythrocytes (as well as of erythrocytes alone) led to specific trapping of lymphocytes in the regional lymph nodes. Preliminary treatment of macrophages loaded with the antigen with antierythrocytic serum decreased the lymphocyte trapping. A supposition was put forward that lymphocytes with receptors on their surface were bound on the macrophage membrane into which the antigen molecules were incorporated.", "PMID": 953284}
{"id": "pubmed23n0032_539", "title": "[State of the hypothalamo-hypophyseal neurosecretory system of rabbits following anaphylactic shock].", "content": "The hypothalamo-hypophysial neurosecretory system of rabbits was investigated 1, 3, 7, 14, and 21 days after the anaphylactic shock. It was found morphometrically that dimensions of the nucleoli of the supraoptic and paraventricular nuclei gradually increased as compared with the moment of anaphylactic shock, but did not reach the control level. Restoration of the size of the nucleoli of the supraoptic nucleus was slower than of the paraventricular one. The quantity of the neurosecretory substance in all the system is significantly higher than in the control up to the end of the observation period (21 days). Thus, the intensity of the processes of synthesis and secretion of the neurohormones in the hypothalamo-hypophysial neurosecretory system was decreased for 21 days of the post-shock period.", "contents": "[State of the hypothalamo-hypophyseal neurosecretory system of rabbits following anaphylactic shock]. The hypothalamo-hypophysial neurosecretory system of rabbits was investigated 1, 3, 7, 14, and 21 days after the anaphylactic shock. It was found morphometrically that dimensions of the nucleoli of the supraoptic and paraventricular nuclei gradually increased as compared with the moment of anaphylactic shock, but did not reach the control level. Restoration of the size of the nucleoli of the supraoptic nucleus was slower than of the paraventricular one. The quantity of the neurosecretory substance in all the system is significantly higher than in the control up to the end of the observation period (21 days). Thus, the intensity of the processes of synthesis and secretion of the neurohormones in the hypothalamo-hypophysial neurosecretory system was decreased for 21 days of the post-shock period.", "PMID": 953285}
{"id": "pubmed23n0032_540", "title": "[Stimulation of humoral immunity to surface antigens of leukemia cells using interferon-containing sera].", "content": "C57BL/6 mice were immunized once with leukemic cells L 1210; (CBAXC57BL/6) F1 mice were immunized once with leukemic cells L 1210 and P-388. These animals were injected intraperitoneally for 8 days (including the day of immunization) with 0.4 ml each of allogeneic or syngeneic interferon-containing serum; as to control animals, they received the same amount of normal serum from the intact mice. The interferon-containing serum was obtained from the (CBAXC57BL/6) F1 mice 24 hours after the intraperitoneal injection of 2 mg of tilorone hydrochloride. Some of the samples of the interferon-containing serum were subjected to the 48-hour dialysis against the physiological saline. The interferon titer in the serum was 512-1500 units/ml. The sera from mice were tested in the microcytotoxic test against leukemic cells 9-10 days after the immunization. A distinct stimulation of the cytotoxic activity of the sera from mice given the interferon-containing serum was observed. Syngeneic interferon-containing serum caused a more marked immunity-stimulating effect than allogeneic serum.", "contents": "[Stimulation of humoral immunity to surface antigens of leukemia cells using interferon-containing sera]. C57BL/6 mice were immunized once with leukemic cells L 1210; (CBAXC57BL/6) F1 mice were immunized once with leukemic cells L 1210 and P-388. These animals were injected intraperitoneally for 8 days (including the day of immunization) with 0.4 ml each of allogeneic or syngeneic interferon-containing serum; as to control animals, they received the same amount of normal serum from the intact mice. The interferon-containing serum was obtained from the (CBAXC57BL/6) F1 mice 24 hours after the intraperitoneal injection of 2 mg of tilorone hydrochloride. Some of the samples of the interferon-containing serum were subjected to the 48-hour dialysis against the physiological saline. The interferon titer in the serum was 512-1500 units/ml. The sera from mice were tested in the microcytotoxic test against leukemic cells 9-10 days after the immunization. A distinct stimulation of the cytotoxic activity of the sera from mice given the interferon-containing serum was observed. Syngeneic interferon-containing serum caused a more marked immunity-stimulating effect than allogeneic serum.", "PMID": 953286}
{"id": "pubmed23n0032_541", "title": "[Completeness of skin regeneration].", "content": "Healing of the wound involving full thickness of the skin was investigated in carnivorous mammals (cats, foxes, polar foxes). Square wounds (2.2 cm2) were inflicted on the back and the head of cats and onthe thigh of foxes and polar foxes. In all the animals investigated the wounds closed chiefly by contracture of their margins. A small regenerate was formed in the centre of the wounds; its structure was almost the same as that of the intact skin: there were hair and sebaceous glands. Connective tissue skeleton of the regenerate resembled the derma by the fiber distribution.", "contents": "[Completeness of skin regeneration]. Healing of the wound involving full thickness of the skin was investigated in carnivorous mammals (cats, foxes, polar foxes). Square wounds (2.2 cm2) were inflicted on the back and the head of cats and onthe thigh of foxes and polar foxes. In all the animals investigated the wounds closed chiefly by contracture of their margins. A small regenerate was formed in the centre of the wounds; its structure was almost the same as that of the intact skin: there were hair and sebaceous glands. Connective tissue skeleton of the regenerate resembled the derma by the fiber distribution.", "PMID": 953287}
{"id": "pubmed23n0032_542", "title": "[Extracorporeal fertilization and cleavage of human oocytes].", "content": "A method of extracorporeal cultivation and fertilization of human oocytes is described. A special method of selection of active spermatozoa was used. The zygote formed 24-36 hours after the fertilization of oocytes; cleavage of two blastomeres occurred in 36-40 hours. Formation of the four-cell stage embryos was observed in 48 hours. The number of blastomeres reached eight in 60-64 hours and the morula formed in 100-120 hours. The results obtained in this work open opportunities for wide study of physiology and pathology of early human embryonic development.", "contents": "[Extracorporeal fertilization and cleavage of human oocytes]. A method of extracorporeal cultivation and fertilization of human oocytes is described. A special method of selection of active spermatozoa was used. The zygote formed 24-36 hours after the fertilization of oocytes; cleavage of two blastomeres occurred in 36-40 hours. Formation of the four-cell stage embryos was observed in 48 hours. The number of blastomeres reached eight in 60-64 hours and the morula formed in 100-120 hours. The results obtained in this work open opportunities for wide study of physiology and pathology of early human embryonic development.", "PMID": 953288}
{"id": "pubmed23n0032_543", "title": "[Cytologic analysis of the secretory epithelium of submaxillary salivary glands hypertrophied by repeated amputation of the lower incisors in rats].", "content": "Repeated amputation of the lower incisor in August line rats induced hypertrophy of the submandibular and sublingual salivary glands. This was true hypertrophy accompanied by hypertrophy of the structural units (acini, cells, nuclei). Cell hypertrophy depended on the hypertrophy of the organellae and the accumulation of secretory materials in the cells. Hypertrophy of the nuclei of acinar cells was conditioned by polyploidisation. Polyploidisation and the changes in the cell ulctrastructure in the submandibular salivary glands pointed to the increase in the functional activity of the organ.", "contents": "[Cytologic analysis of the secretory epithelium of submaxillary salivary glands hypertrophied by repeated amputation of the lower incisors in rats]. Repeated amputation of the lower incisor in August line rats induced hypertrophy of the submandibular and sublingual salivary glands. This was true hypertrophy accompanied by hypertrophy of the structural units (acini, cells, nuclei). Cell hypertrophy depended on the hypertrophy of the organellae and the accumulation of secretory materials in the cells. Hypertrophy of the nuclei of acinar cells was conditioned by polyploidisation. Polyploidisation and the changes in the cell ulctrastructure in the submandibular salivary glands pointed to the increase in the functional activity of the organ.", "PMID": 953289}
{"id": "pubmed23n0032_544", "title": "[Changes in the ultrastructure of human epidermis as a result of freezing].", "content": "Freezing at -35 degrees C and -70 degrees C of unfixed human epidermis brings about an electron microscopic increase of intercellular spaces, a destruction of cellular membranes, homogenization of tonofibrils, and a decrease in the volume of mitochondria with a partial destruction of their inner surface. Nuclear changes are characterized by disruption of nuclear membranes and by massive passage of nucleoproteids into the cytoplasm. Destruction and fragmentation of cytolemma and nuclear membranes is accompanied by formation of myelin-like figures. Freezing at -70 degrees C leads to greater changes in the structure of the epidermis cells, and is accompanied by the appearance of vacuoles of different sizes and shape in the horny scales and in the cytoplasm of some epidermal cells. This is probably due to formation of ice crystals.", "contents": "[Changes in the ultrastructure of human epidermis as a result of freezing]. Freezing at -35 degrees C and -70 degrees C of unfixed human epidermis brings about an electron microscopic increase of intercellular spaces, a destruction of cellular membranes, homogenization of tonofibrils, and a decrease in the volume of mitochondria with a partial destruction of their inner surface. Nuclear changes are characterized by disruption of nuclear membranes and by massive passage of nucleoproteids into the cytoplasm. Destruction and fragmentation of cytolemma and nuclear membranes is accompanied by formation of myelin-like figures. Freezing at -70 degrees C leads to greater changes in the structure of the epidermis cells, and is accompanied by the appearance of vacuoles of different sizes and shape in the horny scales and in the cytoplasm of some epidermal cells. This is probably due to formation of ice crystals.", "PMID": 953290}
{"id": "pubmed23n0032_545", "title": "[Response of cerebral vessels to noradrenaline under hypocapnic conditions and inhibition of prostaglandin biosynthesis].", "content": "Acute experiments were conducted on cats; vasoconstrictor action of noradrenaline on the cerebral vessels and arterial pressure were considerably depressed under conditions of hypocapnia. Inhibition of prostaglandin biosynthesis with indomethacin against the background of continuing hypocapnia promoted marked elevation of cerebrovascular resistance and of arterial pressure in response to intracarotid noradrenaline injection. A hypothetical scheme of prostaglandin participation in the regulation of the tone of the cerebral vessels in hypocapnia and adrenergic actions is presented.", "contents": "[Response of cerebral vessels to noradrenaline under hypocapnic conditions and inhibition of prostaglandin biosynthesis]. Acute experiments were conducted on cats; vasoconstrictor action of noradrenaline on the cerebral vessels and arterial pressure were considerably depressed under conditions of hypocapnia. Inhibition of prostaglandin biosynthesis with indomethacin against the background of continuing hypocapnia promoted marked elevation of cerebrovascular resistance and of arterial pressure in response to intracarotid noradrenaline injection. A hypothetical scheme of prostaglandin participation in the regulation of the tone of the cerebral vessels in hypocapnia and adrenergic actions is presented.", "PMID": 953291}
{"id": "pubmed23n0032_546", "title": "[Microcirculatory and vascular permeability disorders during short-term immobilization stress].", "content": "Immobilization of rats for 1 or 3 hours increased the vascular permeability for colloid carbon particles and led to disturbances of the microcirculation in microvessels (formation of aggregates, appearance of \"plasmatic vessels\", opening of arteriolo-venular shunts). A prominent role of histamine, released from mast cells without any degranulation of the cells, in the pathogenesis of these disturbances was shown. It is supposed that the histamine release served as the trigger mechanism in the development of vascular permeability disturbances in the course of prolonged immobilization stress.", "contents": "[Microcirculatory and vascular permeability disorders during short-term immobilization stress]. Immobilization of rats for 1 or 3 hours increased the vascular permeability for colloid carbon particles and led to disturbances of the microcirculation in microvessels (formation of aggregates, appearance of \"plasmatic vessels\", opening of arteriolo-venular shunts). A prominent role of histamine, released from mast cells without any degranulation of the cells, in the pathogenesis of these disturbances was shown. It is supposed that the histamine release served as the trigger mechanism in the development of vascular permeability disturbances in the course of prolonged immobilization stress.", "PMID": 953292}
{"id": "pubmed23n0032_547", "title": "E1Age and the development of coronary insufficiency under the influence of vasopressin.", "content": "Experimental results showed that the ECG changes (elevation of T wave, a shift of the S-T segment and disturbances of the atrio-ventricular conduction) under the effect of vasopressin occurred in rats aged 24-26 months after lower doses of vasopressin than in the animals aged 10-12 months. With the administration of the same doses coronary insufficiency was more pronounced in the old animals (more frequent disturbances of the atrio-ventricular conduction, grade II or III, and greater derangement of general hemodynamics).", "contents": "E1Age and the development of coronary insufficiency under the influence of vasopressin. Experimental results showed that the ECG changes (elevation of T wave, a shift of the S-T segment and disturbances of the atrio-ventricular conduction) under the effect of vasopressin occurred in rats aged 24-26 months after lower doses of vasopressin than in the animals aged 10-12 months. With the administration of the same doses coronary insufficiency was more pronounced in the old animals (more frequent disturbances of the atrio-ventricular conduction, grade II or III, and greater derangement of general hemodynamics).", "PMID": 953293}
{"id": "pubmed23n0032_548", "title": "[Experimental pain and pruritic syndromes of thalamic origin].", "content": "Pain and itch syndromes of thalamic origin were experimentally induced in rats by creation of generators of pathologically increased excitation in the gelatinose thalamic nucleus by local disturbance of the gelatinose thalamic nucleus by local distrubance of the inhibitory mechanisms in this nucleus with the aid of tetanus toxin. The latter was injected into the mentioned nucleus in the microvolumes using sterotaxis technique. The results of investigations agree with the concept of generator mechanisms of the neuropathological syndromes and the role of hyperactive determinant dispatch stations in the central nervous system activity.", "contents": "[Experimental pain and pruritic syndromes of thalamic origin]. Pain and itch syndromes of thalamic origin were experimentally induced in rats by creation of generators of pathologically increased excitation in the gelatinose thalamic nucleus by local disturbance of the gelatinose thalamic nucleus by local distrubance of the inhibitory mechanisms in this nucleus with the aid of tetanus toxin. The latter was injected into the mentioned nucleus in the microvolumes using sterotaxis technique. The results of investigations agree with the concept of generator mechanisms of the neuropathological syndromes and the role of hyperactive determinant dispatch stations in the central nervous system activity.", "PMID": 953295}
{"id": "pubmed23n0032_549", "title": "[State of the thyroid gland under hypokinetic conditions in rats preliminarily adapted to hypoxia].", "content": "A 50-day hypokinesia caused in rats a sharp depression of the functional activity; an 80-day adaptation to hypoxia led to a moderate thyroid gland hypofunction without any pathological changes in the thyroid parenchyma. Adaptation to hypoxia beginning one month before hypokinesia produced a prophylactic effect decreasing the pathohistological influence of hypokinesia on the thyroid gland function.", "contents": "[State of the thyroid gland under hypokinetic conditions in rats preliminarily adapted to hypoxia]. A 50-day hypokinesia caused in rats a sharp depression of the functional activity; an 80-day adaptation to hypoxia led to a moderate thyroid gland hypofunction without any pathological changes in the thyroid parenchyma. Adaptation to hypoxia beginning one month before hypokinesia produced a prophylactic effect decreasing the pathohistological influence of hypokinesia on the thyroid gland function.", "PMID": 953296}
{"id": "pubmed23n0032_550", "title": "[Increased thymidine excretion under the influence of polyanions].", "content": "During the first 24 hours following injection of polyanions (dextran sulphate in a dose of 62.5 mg per 1 kg of body weight; poly I. poly C in a dose of 2.5 mg per 1 kg of body weight) to rats and mongrel albino mice an increased urinary excretion of thymidine was revealed which occurred after their exposure to high X-ray doses. An increase in urinary excretion of thymidine is assumed to be due to migration of lymphocytes to the blood channel.", "contents": "[Increased thymidine excretion under the influence of polyanions]. During the first 24 hours following injection of polyanions (dextran sulphate in a dose of 62.5 mg per 1 kg of body weight; poly I. poly C in a dose of 2.5 mg per 1 kg of body weight) to rats and mongrel albino mice an increased urinary excretion of thymidine was revealed which occurred after their exposure to high X-ray doses. An increase in urinary excretion of thymidine is assumed to be due to migration of lymphocytes to the blood channel.", "PMID": 953297}
{"id": "pubmed23n0032_551", "title": "[Heart lesions in mice with experimental infection caused by Coxsackie virus A13].", "content": "The results of pathomorphological and virological studies of experimental Coxsackie A 13 virus infection in BALB/C mice are described. Chronic myocarditis, endocarditis and valvulitis were observed in mice inoculated 10 to 12 hours after birth. The virus was revealed in the infected mice for 15-20 days only.", "contents": "[Heart lesions in mice with experimental infection caused by Coxsackie virus A13]. The results of pathomorphological and virological studies of experimental Coxsackie A 13 virus infection in BALB/C mice are described. Chronic myocarditis, endocarditis and valvulitis were observed in mice inoculated 10 to 12 hours after birth. The virus was revealed in the infected mice for 15-20 days only.", "PMID": 953298}
{"id": "pubmed23n0032_552", "title": "[Collagen metabolism in the skin with different vitamin K regimens].", "content": "Secondary vitamin K deficiency in rats caused by pelentan was accompanied by a decrease in the total collagen content and a rise in free oxyproline in the skin. Under these conditions the rate of collagen hydrolysis proved to increase. Vitamin K (vikasol) prevented development of the mentioned shifts in collagen metabolism.", "contents": "[Collagen metabolism in the skin with different vitamin K regimens]. Secondary vitamin K deficiency in rats caused by pelentan was accompanied by a decrease in the total collagen content and a rise in free oxyproline in the skin. Under these conditions the rate of collagen hydrolysis proved to increase. Vitamin K (vikasol) prevented development of the mentioned shifts in collagen metabolism.", "PMID": 953299}
{"id": "pubmed23n0032_553", "title": "[Effect of total gamma-irradiation on the rate of steroid hormone hydroxylation by rat liver microsomes].", "content": "gamma-irradiation in doses of over 600 r proved to decrease the rate of androgen hydroxylation significantly; the rate of hydroxylation of the estrogens under study altered but little. Under the mentioned irradiation conditions the content of cytochrome P-450 in rat hepatic microsomes diminished. A reduction of the hydroxylation rate of steroid hormones by rat hepatic microsomes under the effect of total gamma-irradiation apparently depended on the qualitative changes of cytochrome P-450.", "contents": "[Effect of total gamma-irradiation on the rate of steroid hormone hydroxylation by rat liver microsomes]. gamma-irradiation in doses of over 600 r proved to decrease the rate of androgen hydroxylation significantly; the rate of hydroxylation of the estrogens under study altered but little. Under the mentioned irradiation conditions the content of cytochrome P-450 in rat hepatic microsomes diminished. A reduction of the hydroxylation rate of steroid hormones by rat hepatic microsomes under the effect of total gamma-irradiation apparently depended on the qualitative changes of cytochrome P-450.", "PMID": 953300}
{"id": "pubmed23n0032_554", "title": "[Dynamics of cardiac and skeletal muscle lactate dehydrogenase activity following a single exposure to an alternating magnetic field].", "content": "A rise in LDH activity and a change of the enzyme distribution in the cytostructures of the heart and skeletal muscles of albino rats was revealed during the first 48 hours after a single twenty-four-hour action of an A. C. magnetic field (200 e, 50 cps). A displacement of the enzyma ratio in the direction of M-type was noted. Complete normalization occurred in the 3rd or 4th week only.", "contents": "[Dynamics of cardiac and skeletal muscle lactate dehydrogenase activity following a single exposure to an alternating magnetic field]. A rise in LDH activity and a change of the enzyme distribution in the cytostructures of the heart and skeletal muscles of albino rats was revealed during the first 48 hours after a single twenty-four-hour action of an A. C. magnetic field (200 e, 50 cps). A displacement of the enzyma ratio in the direction of M-type was noted. Complete normalization occurred in the 3rd or 4th week only.", "PMID": 953301}
{"id": "pubmed23n0032_555", "title": "[Structural derangements in deoxyribonucleoprotein complex damaged by N-nitroso-N-methylurea].", "content": "The effect of deoxyribonucleoproteid (DNP) solubilization under conditions of the ionic strength of the medium approaching the physiological one depended greatly after the treatment with a mutagen - N-nitroso-N-methylurea (NMU) on the NMU concentration. Apparently, for the change of DNA into a soluble state the critical number of groups in the DNA and protein should be modified. On the basis of the data obtained by circular dichroism and viscosimetry a conclusion was drawn that after the NMU treatment the DNP complex became soluble in the solvents with the ionic strength approaching the physiological one, chiefly as the result of labilization and dissociation of DNA-protein bonds.", "contents": "[Structural derangements in deoxyribonucleoprotein complex damaged by N-nitroso-N-methylurea]. The effect of deoxyribonucleoproteid (DNP) solubilization under conditions of the ionic strength of the medium approaching the physiological one depended greatly after the treatment with a mutagen - N-nitroso-N-methylurea (NMU) on the NMU concentration. Apparently, for the change of DNA into a soluble state the critical number of groups in the DNA and protein should be modified. On the basis of the data obtained by circular dichroism and viscosimetry a conclusion was drawn that after the NMU treatment the DNP complex became soluble in the solvents with the ionic strength approaching the physiological one, chiefly as the result of labilization and dissociation of DNA-protein bonds.", "PMID": 953302}
{"id": "pubmed23n0032_556", "title": "[Effect of the protease inhibitor--contrical--on granulation tissue metabolism].", "content": "Studies of the influence of contrycal on metabolism of rat granulation tissue showed that this protease inhibitor stimulated C14-glycine incorporation into collagen proteins and caused reorganization of the isoenzyme LDH spectrum; at the same time there was a delay in the maturation of the granulation tissue with its marked growth.", "contents": "[Effect of the protease inhibitor--contrical--on granulation tissue metabolism]. Studies of the influence of contrycal on metabolism of rat granulation tissue showed that this protease inhibitor stimulated C14-glycine incorporation into collagen proteins and caused reorganization of the isoenzyme LDH spectrum; at the same time there was a delay in the maturation of the granulation tissue with its marked growth.", "PMID": 953303}
{"id": "pubmed23n0032_557", "title": "[Effect of prostaglandin F2alpha on the lysosomal membranes of eye tissues].", "content": "It was demonstrated that both in vitro and in intravenous injection of prostaglandin F2alpha there occurred labilization of the membranes of sclera and ciliary body lysosomes, in difference from the cornea in rabbits. Glycosidase activity appeared in the vitreous body under the effect of prostaglandin, which was absent under normal conditions.", "contents": "[Effect of prostaglandin F2alpha on the lysosomal membranes of eye tissues]. It was demonstrated that both in vitro and in intravenous injection of prostaglandin F2alpha there occurred labilization of the membranes of sclera and ciliary body lysosomes, in difference from the cornea in rabbits. Glycosidase activity appeared in the vitreous body under the effect of prostaglandin, which was absent under normal conditions.", "PMID": 953304}
{"id": "pubmed23n0032_558", "title": "[Effect of dexamethasone on RNA synthesis in blood lymphocytes stimulated by phytohemagglutinin].", "content": "Phytohemagglutinin (PHA) stimulated RNA synthesis 6 hours after the beginning of incubation of blood lymphocytes in vitro. Dexamethazone in a concentration of 60 mug/ml depressed the RNA synthesis in the blood lymphocytes of donors, treated with PHA, 6 hours after the beginning of cell incubation with the hormone and PHA in vitro. Changes in the action mechanism of the hormone which can lead to the change of sensitivity to glucocorticoids of the same cells at various stages of the cell cycle are analyzed.", "contents": "[Effect of dexamethasone on RNA synthesis in blood lymphocytes stimulated by phytohemagglutinin]. Phytohemagglutinin (PHA) stimulated RNA synthesis 6 hours after the beginning of incubation of blood lymphocytes in vitro. Dexamethazone in a concentration of 60 mug/ml depressed the RNA synthesis in the blood lymphocytes of donors, treated with PHA, 6 hours after the beginning of cell incubation with the hormone and PHA in vitro. Changes in the action mechanism of the hormone which can lead to the change of sensitivity to glucocorticoids of the same cells at various stages of the cell cycle are analyzed.", "PMID": 953305}
{"id": "pubmed23n0032_559", "title": "[\"Molecular organization\" of the vagus nerves and cardiac muscle in different functional states].", "content": "On the example of the system vagus nerves - the myocardium of rabbits the author considers structural changes in the molecular substrate connected with electrical stimulation of one of the nerves varying by strength. Mitogenetic radiation of the nerve in experiments in situ permitted to assess the increase (or decrease) of the unbalanced structural conditions - molecular constellations. In subthreshold stimulation the quantity of unbalanced constellations proved to increase. Physiological significance of this phenomenon is discussed.", "contents": "[\"Molecular organization\" of the vagus nerves and cardiac muscle in different functional states]. On the example of the system vagus nerves - the myocardium of rabbits the author considers structural changes in the molecular substrate connected with electrical stimulation of one of the nerves varying by strength. Mitogenetic radiation of the nerve in experiments in situ permitted to assess the increase (or decrease) of the unbalanced structural conditions - molecular constellations. In subthreshold stimulation the quantity of unbalanced constellations proved to increase. Physiological significance of this phenomenon is discussed.", "PMID": 953306}
{"id": "pubmed23n0032_560", "title": "[Effect of narcotic analgesics on impulse conduction along the afferent pathways of visceral nerves].", "content": "Experiments were conducted on chloralose-anesthetized cats. The action of morphine and promedol upon the potentials of the cortical and subcortical structures occurring after the visceral nerve stimulation was studied. Morphine proved to depress the potentials evoked by stimulation of the inferior cardiac and vagus nerves, in the specific, associative and nonspecific structures of the brain; promedol produced an analogous effect. Morphine also inhibited the potentials occurring after the stimulation of the splanchnic nerve in the associative and nonspecific structures; depression of the responses in the specific pathways was less pronounced.", "contents": "[Effect of narcotic analgesics on impulse conduction along the afferent pathways of visceral nerves]. Experiments were conducted on chloralose-anesthetized cats. The action of morphine and promedol upon the potentials of the cortical and subcortical structures occurring after the visceral nerve stimulation was studied. Morphine proved to depress the potentials evoked by stimulation of the inferior cardiac and vagus nerves, in the specific, associative and nonspecific structures of the brain; promedol produced an analogous effect. Morphine also inhibited the potentials occurring after the stimulation of the splanchnic nerve in the associative and nonspecific structures; depression of the responses in the specific pathways was less pronounced.", "PMID": 953307}
{"id": "pubmed23n0032_561", "title": "[Enhancement of the immune response following immunization with L1210 cells preliminarily incubated with interferon].", "content": "A single intraperitioneal injection of L 1210 cells preincubated in interferon or in \"false interferon\" was given to mice. These mice were sacrificed on the 10th day, and cytotoxicity of the sera against the L 1210 leukemic cells vas determined in the microcytotoxic test (modification of Terasaki's method). Leukemic cells treated with interferon before the immunization caused a more pronounced immune response in comparison with the cells treated under the same conditions with \"false interferon\". By the results of cytotoxic reaction immune response was enhanced 2-4-fold.", "contents": "[Enhancement of the immune response following immunization with L1210 cells preliminarily incubated with interferon]. A single intraperitioneal injection of L 1210 cells preincubated in interferon or in \"false interferon\" was given to mice. These mice were sacrificed on the 10th day, and cytotoxicity of the sera against the L 1210 leukemic cells vas determined in the microcytotoxic test (modification of Terasaki's method). Leukemic cells treated with interferon before the immunization caused a more pronounced immune response in comparison with the cells treated under the same conditions with \"false interferon\". By the results of cytotoxic reaction immune response was enhanced 2-4-fold.", "PMID": 953308}
{"id": "pubmed23n0032_562", "title": "[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].", "content": "The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.", "contents": "[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes]. The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries. Dead cells did not pass from the capillaries into the medium. Tuberculin and BCG vaccine inhibited the migration of macrophages of guinea pigs immunized with BCG, but not with staphylococcus allergen.", "PMID": 953309}
{"id": "pubmed23n0032_563", "title": "[Antituberculosis immunity in adult thymectomized mice].", "content": "Thymectomy performed in adult animals 6 months and more before the inoculation of mycobacteria H37Rv reduced the resistance to tuberculosis infection considerably. This reduction was the greater, the more time passed after the operation. There also developed immunity disturbances mainly connected with the damage of the thymus-dependent cells (depletion of thymus-dependent zones, reduction of tuberculin sensitivity of the skin and of the cytotoxic effect of lymphocytes on antigen-containing target cells).", "contents": "[Antituberculosis immunity in adult thymectomized mice]. Thymectomy performed in adult animals 6 months and more before the inoculation of mycobacteria H37Rv reduced the resistance to tuberculosis infection considerably. This reduction was the greater, the more time passed after the operation. There also developed immunity disturbances mainly connected with the damage of the thymus-dependent cells (depletion of thymus-dependent zones, reduction of tuberculin sensitivity of the skin and of the cytotoxic effect of lymphocytes on antigen-containing target cells).", "PMID": 953310}
{"id": "pubmed23n0032_564", "title": "[Immunochemical identification of a new embryonal antigen in ovarian tumor tissue].", "content": "A new embryonic antigen was found in the ovarian tumour tissues by immunodiffusion methods. This antigen consists of two components with a different electrophoretic mobility: a low peak migrates in the alha0-globulin zone, and a rapid one - in the prealbumin zone; it differs immunologically from alpha-fetoprot ein and cancer embrynoic antigen of the large intestine. This antigen was found in the fetus and newborn blood serum and also in the tissue extracts of ovarian tumours. No such antigen was revealed in the normal tissues of adults, or in the blood serum of donors and oncological patients.", "contents": "[Immunochemical identification of a new embryonal antigen in ovarian tumor tissue]. A new embryonic antigen was found in the ovarian tumour tissues by immunodiffusion methods. This antigen consists of two components with a different electrophoretic mobility: a low peak migrates in the alha0-globulin zone, and a rapid one - in the prealbumin zone; it differs immunologically from alpha-fetoprot ein and cancer embrynoic antigen of the large intestine. This antigen was found in the fetus and newborn blood serum and also in the tissue extracts of ovarian tumours. No such antigen was revealed in the normal tissues of adults, or in the blood serum of donors and oncological patients.", "PMID": 953311}
{"id": "pubmed23n0032_565", "title": "[Concentration of adenylic nucleotides and respiratory phosphorylation in the mitochondria of rats with transplanted tumors].", "content": "A study was made of adenylic mononucleotides, the process of oxydative phosphorylation and ATP-ase activity of hepatic mitochondria of rats with transplantable sarcoma 45 and Walker carcinosarcoma at various stages of tumour growtli. A reduction of the ATP levels observed in the hepatic mitochondria of tumour-bearing rats was caused, on the one hand, by suppression of its formation as a result of a partial uncoupling of the oxidative phosphorylation, and on the other hand - by intensification of its decomposition as a result of increased ATP-ase activity.", "contents": "[Concentration of adenylic nucleotides and respiratory phosphorylation in the mitochondria of rats with transplanted tumors]. A study was made of adenylic mononucleotides, the process of oxydative phosphorylation and ATP-ase activity of hepatic mitochondria of rats with transplantable sarcoma 45 and Walker carcinosarcoma at various stages of tumour growtli. A reduction of the ATP levels observed in the hepatic mitochondria of tumour-bearing rats was caused, on the one hand, by suppression of its formation as a result of a partial uncoupling of the oxidative phosphorylation, and on the other hand - by intensification of its decomposition as a result of increased ATP-ase activity.", "PMID": 953312}
{"id": "pubmed23n0032_566", "title": "[Myocardial cell ultrastructure of yaks dwelling at high altitudes].", "content": "The ultrastructure of myocardial cells of the left, right ventricle, and the ventricular septum was studied in the Altai and Pamir Yaks permanently living at the altitude of 3000-3600 m. Electron microscopic studies of myocardial cells revealed, along with the normal mitochondria, the ones with a peculiar structure of the cristae; these had the appearance of polyhedral wavy membranes in some groups of the mitochondria, and of polyhedral netted structures - in the others. Considerable accumulations of glycogen granules were found beneath the sarcolemma, in the perinuclear cytoplasmic zone and between the myofibrils. The results suggest that by undulating and creating a certain structural regularity the mitochondrial cristae increased their active area ensuring the efficacy of the mitochondrial function. Considerable accumulation of glycogen granules in the majority of myocardial cells seems to maintain the energy potential of the myocardium preventing the development of hypoxia.", "contents": "[Myocardial cell ultrastructure of yaks dwelling at high altitudes]. The ultrastructure of myocardial cells of the left, right ventricle, and the ventricular septum was studied in the Altai and Pamir Yaks permanently living at the altitude of 3000-3600 m. Electron microscopic studies of myocardial cells revealed, along with the normal mitochondria, the ones with a peculiar structure of the cristae; these had the appearance of polyhedral wavy membranes in some groups of the mitochondria, and of polyhedral netted structures - in the others. Considerable accumulations of glycogen granules were found beneath the sarcolemma, in the perinuclear cytoplasmic zone and between the myofibrils. The results suggest that by undulating and creating a certain structural regularity the mitochondrial cristae increased their active area ensuring the efficacy of the mitochondrial function. Considerable accumulation of glycogen granules in the majority of myocardial cells seems to maintain the energy potential of the myocardium preventing the development of hypoxia.", "PMID": 953313}
{"id": "pubmed23n0032_567", "title": "[Seasonal changes in several ultrastructural indices of lake frog heart ventricle myocytes].", "content": "The method of electron microscopy with morphometric analysis was applied to the study of some ultrastructural indices of frog heart (ventricle) myocardium - the interlocated discs, the lateral junctions and mitochondria - in spring and in autumn. The results obtained pointed to seasonal differences in the cardiomyocyte ultrastructure, this being confirmed by the quantitative analysis.", "contents": "[Seasonal changes in several ultrastructural indices of lake frog heart ventricle myocytes]. The method of electron microscopy with morphometric analysis was applied to the study of some ultrastructural indices of frog heart (ventricle) myocardium - the interlocated discs, the lateral junctions and mitochondria - in spring and in autumn. The results obtained pointed to seasonal differences in the cardiomyocyte ultrastructure, this being confirmed by the quantitative analysis.", "PMID": 953314}
{"id": "pubmed23n0032_568", "title": "[Human oogenesis in organ culture].", "content": "The authors compared the extent of development of the germ cells of the human fetus ovaries developing in the organ culture and of control ovaries obtained of fetuses of \"equivalent\" age. The capacity to enter the meiosis prophase under conditions of the organ culture was expressed by the germ cells of fetuses of 8-9 week of gestation, and older; only individual oocytes reached the leptotene stage in the explants of the ovaries of 7-8 week fetuses. The oocytes at the leptotene, zygotene and pachitene stage appeared in the culture at the same periods as in the organism. However, the percentage of cells of these stages in the explant was as a rule lower than in control ovaries. In case of pulse administration of thymidine-3H into the culture labeled oocytes at the zygotene stage appeared in 4, and at the pachitene stage - in 14 days.", "contents": "[Human oogenesis in organ culture]. The authors compared the extent of development of the germ cells of the human fetus ovaries developing in the organ culture and of control ovaries obtained of fetuses of \"equivalent\" age. The capacity to enter the meiosis prophase under conditions of the organ culture was expressed by the germ cells of fetuses of 8-9 week of gestation, and older; only individual oocytes reached the leptotene stage in the explants of the ovaries of 7-8 week fetuses. The oocytes at the leptotene, zygotene and pachitene stage appeared in the culture at the same periods as in the organism. However, the percentage of cells of these stages in the explant was as a rule lower than in control ovaries. In case of pulse administration of thymidine-3H into the culture labeled oocytes at the zygotene stage appeared in 4, and at the pachitene stage - in 14 days.", "PMID": 953315}
{"id": "pubmed23n0032_569", "title": "[Circadian rhythm of mitoses and number of cells synthesizing DNA following adrenalectomy in mice].", "content": "The diurnal changes in the number of mitoses and DNA-synthesizing cells were studied in intact and adrenalectomized mice. In the corneal epithelium of adrenalectomized mice the diurnal rhythm of DNA-synthesizing cells became biphasic, and the variation amplitude of the 24-hour mitotic activity was sharply intensified. In the liver the diurnal rhythm of DNA-synthesizing cells was similar in the intact and the operated on mice; as to the mitotic rhythm - it became biphasic in the operated on animals. Thus, adrenalectomy deranged the phasic structure of the mitotic and DNA-synthesizing rhythms in the tissues under study and led to dissociation in the entrance of cells into the DNA-synthesis and mitosis.", "contents": "[Circadian rhythm of mitoses and number of cells synthesizing DNA following adrenalectomy in mice]. The diurnal changes in the number of mitoses and DNA-synthesizing cells were studied in intact and adrenalectomized mice. In the corneal epithelium of adrenalectomized mice the diurnal rhythm of DNA-synthesizing cells became biphasic, and the variation amplitude of the 24-hour mitotic activity was sharply intensified. In the liver the diurnal rhythm of DNA-synthesizing cells was similar in the intact and the operated on mice; as to the mitotic rhythm - it became biphasic in the operated on animals. Thus, adrenalectomy deranged the phasic structure of the mitotic and DNA-synthesizing rhythms in the tissues under study and led to dissociation in the entrance of cells into the DNA-synthesis and mitosis.", "PMID": 953316}
{"id": "pubmed23n0032_570", "title": "[Skin regeneration in several species of mammals].", "content": "The healing of full-thickness wounds (1.2 cm2) was studied in minks and sables. The wound closure in both animal species resulted mainly from the contraction of the wound. A small regenerate containing hair and sebaceous glands was formed in the centre of the wound. It is supposed that these hairs developed from the old hair roots transfered with the lower layers of the corium adjoining the wound.", "contents": "[Skin regeneration in several species of mammals]. The healing of full-thickness wounds (1.2 cm2) was studied in minks and sables. The wound closure in both animal species resulted mainly from the contraction of the wound. A small regenerate containing hair and sebaceous glands was formed in the centre of the wound. It is supposed that these hairs developed from the old hair roots transfered with the lower layers of the corium adjoining the wound.", "PMID": 953317}
{"id": "pubmed23n0032_571", "title": "[Mechanism of the damaging effect of chloridine on white rat embryos].", "content": "A study was made of the dynamics of pathohistological changes occurring in the embryos of albino rats under conditions of a single action of teratogenic doses of daraprim of the 9th day of pregnancy. Decidual tissue proved to be the most sensitive to daraprim the first disturbances in this tissue were noted to soon as one hour after the injection. Cells of various embryonic tissues reacted to daraprim differently. Most pronounced cytotoxic effect of daraprim is seen on the embryonic mesoderm and on the extraembryonic ectoderm. A conclusion was drawn on a significant role played by hemodynamic changes associated with disturbances of decidual tissue in the mechanism of the pathogenic action of some chemical teratogens on mammalian embryos.", "contents": "[Mechanism of the damaging effect of chloridine on white rat embryos]. A study was made of the dynamics of pathohistological changes occurring in the embryos of albino rats under conditions of a single action of teratogenic doses of daraprim of the 9th day of pregnancy. Decidual tissue proved to be the most sensitive to daraprim the first disturbances in this tissue were noted to soon as one hour after the injection. Cells of various embryonic tissues reacted to daraprim differently. Most pronounced cytotoxic effect of daraprim is seen on the embryonic mesoderm and on the extraembryonic ectoderm. A conclusion was drawn on a significant role played by hemodynamic changes associated with disturbances of decidual tissue in the mechanism of the pathogenic action of some chemical teratogens on mammalian embryos.", "PMID": 953318}
{"id": "pubmed23n0032_572", "title": "[Adrenergic terminal structures in mamalian mesentery].", "content": "The work is devoted to the study of adrenergic terminal structures in the mesentery of mammals by fluorescent microscopy of catecholamines. The system of adrenergic innervation of the mesentery proved to be based on well-developed perivascular plexuses. In many points of these plexuses individual adrenergic fibers penetrated the avascular mesenteric areas, where they formed preterminal and terminal portions as a result of repeated dichotomic divisions. These innervating structures built by the principle of widespread or restricted arborizations displayed morphological similarity to free sensory nerve endings. In this connection a possibility of distinction of the sensory (afferent) links in the catecholamine-containing vegetative nerve plexuses is discussed.", "contents": "[Adrenergic terminal structures in mamalian mesentery]. The work is devoted to the study of adrenergic terminal structures in the mesentery of mammals by fluorescent microscopy of catecholamines. The system of adrenergic innervation of the mesentery proved to be based on well-developed perivascular plexuses. In many points of these plexuses individual adrenergic fibers penetrated the avascular mesenteric areas, where they formed preterminal and terminal portions as a result of repeated dichotomic divisions. These innervating structures built by the principle of widespread or restricted arborizations displayed morphological similarity to free sensory nerve endings. In this connection a possibility of distinction of the sensory (afferent) links in the catecholamine-containing vegetative nerve plexuses is discussed.", "PMID": 953319}
{"id": "pubmed23n0032_573", "title": "[Microspectrophotometric study of DNA concentration in the nuclei of muscle cells in the peri-infarct zone of the rabbit heart].", "content": "A comparative microspectrophotometric study of the nuclear DNA content in the myocardial cells of the periinfarction region of the left ventricle was made in rabbit hearts during different periods of experimental ischemia. A moderate increase of both the polyploid and amitotically divided nuclei were revealed. This results are discussed from the aspect of possible participation of nuclear polyploidia and mitotic division in the mechanisms of intracellular regeneration of the myocardium.", "contents": "[Microspectrophotometric study of DNA concentration in the nuclei of muscle cells in the peri-infarct zone of the rabbit heart]. A comparative microspectrophotometric study of the nuclear DNA content in the myocardial cells of the periinfarction region of the left ventricle was made in rabbit hearts during different periods of experimental ischemia. A moderate increase of both the polyploid and amitotically divided nuclei were revealed. This results are discussed from the aspect of possible participation of nuclear polyploidia and mitotic division in the mechanisms of intracellular regeneration of the myocardium.", "PMID": 953320}
{"id": "pubmed23n0032_574", "title": "[Interstitial cells of the renal medulla in rats with genetic spontaneous hypertension].", "content": "Electron microscopic studies of interstitial cells (IC) of the renal medulla inspontaneously hypertensive rats (SHR) were carried out. Morphometric analysis revealed no differences in the total volume of the lipid granules of IC in the 1 1/2-month-old SHR and control Wistar rats; one-year-old SHR showed an increased volume of granules, IC of SHR displayed hypertrophy and hyperplasia of both the rough and the smooth endoplasmic reticulum and the Golgi complex, especially marked at the early stage of hypertension. Apparently the state of ultrastructures of the synthesis and secretion should be accepted as a criterion for the assessment of the IC function, at least in arterial hypertension, but not the degree of decreased lipid granularity as supposed formerly.", "contents": "[Interstitial cells of the renal medulla in rats with genetic spontaneous hypertension]. Electron microscopic studies of interstitial cells (IC) of the renal medulla inspontaneously hypertensive rats (SHR) were carried out. Morphometric analysis revealed no differences in the total volume of the lipid granules of IC in the 1 1/2-month-old SHR and control Wistar rats; one-year-old SHR showed an increased volume of granules, IC of SHR displayed hypertrophy and hyperplasia of both the rough and the smooth endoplasmic reticulum and the Golgi complex, especially marked at the early stage of hypertension. Apparently the state of ultrastructures of the synthesis and secretion should be accepted as a criterion for the assessment of the IC function, at least in arterial hypertension, but not the degree of decreased lipid granularity as supposed formerly.", "PMID": 953321}
{"id": "pubmed23n0032_575", "title": "[Electron-cytochemical study of cerebral mitochondria at different periods after death].", "content": "3'3'-diaminobenzidine (DAB) oxidation was studied in the human and rat brain by electron cytochemistry at different periods after death. The product of DAB oxidation localized in mitochondria, lipofucsin pigment granules and erythrocytes. DAB oxidation by rat and human brain mitochondria proved to decrease but slightly even two days after death.", "contents": "[Electron-cytochemical study of cerebral mitochondria at different periods after death]. 3'3'-diaminobenzidine (DAB) oxidation was studied in the human and rat brain by electron cytochemistry at different periods after death. The product of DAB oxidation localized in mitochondria, lipofucsin pigment granules and erythrocytes. DAB oxidation by rat and human brain mitochondria proved to decrease but slightly even two days after death.", "PMID": 953322}
{"id": "pubmed23n0032_576", "title": "[Hydroxyurea synchronization of cellular proliferation in the esophageal epithelium of mice with tumors].", "content": "The synchronizing effect of hydroxyurea on the passage of the esophagus epithelia cells through the stage of the DNA synthesis and mitosis was investigated in albino mice bearing sarcoma. Dinrnal variations in the number of epithelial cells at these stages of the mitotic cycle were taken into consideration. Two mutually complementary indices for the measurement of the shifts in the cell population and the rate of the synchronization are used. Two cell groups which passed the S-phase and mitosis synchronously were found in artificial synchronization in the esophageal epithelium. The synchronization indices characterizing the first group were lower than control. In the second group the cell count during the DNA synthesis was double as compared with the number of cells synthesizing DNA in natural synchronization; however, the rate of the changes in the synchronism was the same in the experimental and control groups.", "contents": "[Hydroxyurea synchronization of cellular proliferation in the esophageal epithelium of mice with tumors]. The synchronizing effect of hydroxyurea on the passage of the esophagus epithelia cells through the stage of the DNA synthesis and mitosis was investigated in albino mice bearing sarcoma. Dinrnal variations in the number of epithelial cells at these stages of the mitotic cycle were taken into consideration. Two mutually complementary indices for the measurement of the shifts in the cell population and the rate of the synchronization are used. Two cell groups which passed the S-phase and mitosis synchronously were found in artificial synchronization in the esophageal epithelium. The synchronization indices characterizing the first group were lower than control. In the second group the cell count during the DNA synthesis was double as compared with the number of cells synthesizing DNA in natural synchronization; however, the rate of the changes in the synchronism was the same in the experimental and control groups.", "PMID": 953323}
{"id": "pubmed23n0032_577", "title": "[Several methodologic features of prolonged human lymphocyte storage].", "content": "When using in the lymphocytotoxic reaction lymphocytes stored in frozen condition the proportion of perished cells after thawing should not exceed 10-20%. Therefore, a study was made of the influence of various concentrations of dimethylsulfoxide (DMSO), composition of the medium and of other parameters for the purpose of elaboration of the optimal conditions of freezing human lymphocytes. The best results were obtained in freezing cells in the autologous serum with 12.5% DMSO. However sufficiently favourable results were also seen in using AB serum and 20% bovine serum in Eagle's medium.", "contents": "[Several methodologic features of prolonged human lymphocyte storage]. When using in the lymphocytotoxic reaction lymphocytes stored in frozen condition the proportion of perished cells after thawing should not exceed 10-20%. Therefore, a study was made of the influence of various concentrations of dimethylsulfoxide (DMSO), composition of the medium and of other parameters for the purpose of elaboration of the optimal conditions of freezing human lymphocytes. The best results were obtained in freezing cells in the autologous serum with 12.5% DMSO. However sufficiently favourable results were also seen in using AB serum and 20% bovine serum in Eagle's medium.", "PMID": 953325}
{"id": "pubmed23n0032_578", "title": "[Serotonin concentration in the hypothalamus following alteration of pituitary thyrotropic function].", "content": "The hypothalamic serotonin content in the altered pituitary thyrotropic function was determined in mature male rabbits by the fluorescent method. No distinct correlation was found between the intensity of the pituitary thyrotropic function and serotonin level in the hypothalamus. Intensification of the hypophysial thyrotropic function under the effect of 6-methylthiouracil or partial thyroidectomy was accompanied by a rise in serotonin concentration, while in aseptic inflammation in the thyroid gland, or in combination of the upper cervical sympathetic ganglia ablation with chlorpromazine administration this adenohypophysial function proved to increase without any significant shifts in the hypothalamic serotonin level. The mentioned findings suggest that the role of serotonin in the hypothalamic regulation of the hypophysial thyrotropic function could not be considered as determining.", "contents": "[Serotonin concentration in the hypothalamus following alteration of pituitary thyrotropic function]. The hypothalamic serotonin content in the altered pituitary thyrotropic function was determined in mature male rabbits by the fluorescent method. No distinct correlation was found between the intensity of the pituitary thyrotropic function and serotonin level in the hypothalamus. Intensification of the hypophysial thyrotropic function under the effect of 6-methylthiouracil or partial thyroidectomy was accompanied by a rise in serotonin concentration, while in aseptic inflammation in the thyroid gland, or in combination of the upper cervical sympathetic ganglia ablation with chlorpromazine administration this adenohypophysial function proved to increase without any significant shifts in the hypothalamic serotonin level. The mentioned findings suggest that the role of serotonin in the hypothalamic regulation of the hypophysial thyrotropic function could not be considered as determining.", "PMID": 953326}
{"id": "pubmed23n0032_579", "title": "[The role of adrenocortical response to physical stress on the body's work capacity].", "content": "Adrenocortical reaction to muscular exertion was inhibited by the intramuscular injection of 125mg of dexamethasone for 2 days of every week of the training. The maximal swimming time in the water (33--34 degrees C) with an additional load of 3 per cent of body weight failed to increase after 5 weeks of training in the animals to which dexamethasome was infected. In these animals the blood corticosterone level increased less after the maximal swimming test than in rats trained without any dexamethasone administration.", "contents": "[The role of adrenocortical response to physical stress on the body's work capacity]. Adrenocortical reaction to muscular exertion was inhibited by the intramuscular injection of 125mg of dexamethasone for 2 days of every week of the training. The maximal swimming time in the water (33--34 degrees C) with an additional load of 3 per cent of body weight failed to increase after 5 weeks of training in the animals to which dexamethasome was infected. In these animals the blood corticosterone level increased less after the maximal swimming test than in rats trained without any dexamethasone administration.", "PMID": 953327}
{"id": "pubmed23n0032_580", "title": "[Role of the splanchnic nerves in regulating maximal glucose transport in the kidneys].", "content": "Glomerular filtration in the kidney decreases at side of the splanchnic nerve stimulation; as to the maximal glucose reabsorption -- it remains unchanged. After demedullation of the adrenal glands stimulation of the splanchnic nerve increases the filtration in the contralateral kidney, without altering the maximal glucose transport. Adrenergic fibers of the splanchnic nerve produce no direct action on the maximal glucose transport in the kidneys.", "contents": "[Role of the splanchnic nerves in regulating maximal glucose transport in the kidneys]. Glomerular filtration in the kidney decreases at side of the splanchnic nerve stimulation; as to the maximal glucose reabsorption -- it remains unchanged. After demedullation of the adrenal glands stimulation of the splanchnic nerve increases the filtration in the contralateral kidney, without altering the maximal glucose transport. Adrenergic fibers of the splanchnic nerve produce no direct action on the maximal glucose transport in the kidneys.", "PMID": 953328}
{"id": "pubmed23n0032_581", "title": "[Potentiation and restitution of the myocardial contractility of rats adapted to physical stress].", "content": "Initial contractile amplitude of the myocardial strips determined the inotropic response to paired stimulation. The higher the initial amplitude -- the lesser the degree of potentiation and the greater the degree of contraction restitution. At equal amplitudes the myocardial strips taken from rats adapted to exercise showed a greater degree of potentiation and a lesser degree of restitution as compared to control. These changes appear to be due to the changes in the ionic transport in the myocardial cells.", "contents": "[Potentiation and restitution of the myocardial contractility of rats adapted to physical stress]. Initial contractile amplitude of the myocardial strips determined the inotropic response to paired stimulation. The higher the initial amplitude -- the lesser the degree of potentiation and the greater the degree of contraction restitution. At equal amplitudes the myocardial strips taken from rats adapted to exercise showed a greater degree of potentiation and a lesser degree of restitution as compared to control. These changes appear to be due to the changes in the ionic transport in the myocardial cells.", "PMID": 953329}
{"id": "pubmed23n0032_582", "title": "[Influence of salivary kallikreins on microvessels of the hamster cheek pouch and rat mesentery].", "content": "By means of in vivo microscopy the influence of human, rat, and hamster purified salivary kallikreins upon the microvessels was studied in rat mesentery and hamster cheek pouch. Dilatation of arterioles, a decrease of the venule diameter, and an increase in the number of active capillaries was revealed. The species and organ peculiarities of these effects were described. The mesentery and the cheek pouch homogenates, albumin proteins, histamine and some amino acids were capable of activating the salivary kallikreins.", "contents": "[Influence of salivary kallikreins on microvessels of the hamster cheek pouch and rat mesentery]. By means of in vivo microscopy the influence of human, rat, and hamster purified salivary kallikreins upon the microvessels was studied in rat mesentery and hamster cheek pouch. Dilatation of arterioles, a decrease of the venule diameter, and an increase in the number of active capillaries was revealed. The species and organ peculiarities of these effects were described. The mesentery and the cheek pouch homogenates, albumin proteins, histamine and some amino acids were capable of activating the salivary kallikreins.", "PMID": 953330}
{"id": "pubmed23n0032_583", "title": "[Adrenal cortical function in rats with traumatic shock following removal and deafferentation of the hypothalamus].", "content": "The 11-oxycorticosteroid (11-OCS) content was studied in the blood plasma of sham-operated rats, rats with intact, ablated and deafferentated medial-basal hypothalamus (MBH) in traumatic shock. There were no reliable differences in the 11-OCS basal level in various groups of rats. MBH ablation led to the reduction in the weight of adrenal and pituitary glands and MBH deafferentation -- to increase in the weight of the former. The 11-OCS level was reliably higher in traumatic shock of rats with complete MBH deafferentation than in sham-operated animals.", "contents": "[Adrenal cortical function in rats with traumatic shock following removal and deafferentation of the hypothalamus]. The 11-oxycorticosteroid (11-OCS) content was studied in the blood plasma of sham-operated rats, rats with intact, ablated and deafferentated medial-basal hypothalamus (MBH) in traumatic shock. There were no reliable differences in the 11-OCS basal level in various groups of rats. MBH ablation led to the reduction in the weight of adrenal and pituitary glands and MBH deafferentation -- to increase in the weight of the former. The 11-OCS level was reliably higher in traumatic shock of rats with complete MBH deafferentation than in sham-operated animals.", "PMID": 953331}
{"id": "pubmed23n0032_584", "title": "[Pathogenesis of the low cardiac output syndrome in postreanimation states].", "content": "Functional reserves of the cardiovascular system during sharp depression of the cardiac output  in the postreanimation period after 15-minute cardiac arrest were studied in experiments on dogs (by loading with different fluid volumes). Acute hypervolemia did not produce any circulatory decompensation. Reinforcement of venous return and changes in the peripheral circulation due to polyglucine loading augmented the CVP temporarily, and produced a stable increase of the AP, of the cardiac output, systolic volume, the work of the left cardiac ventricle and of the total oxygen consumption by the organism. Meanwhile there was a decrease of the peripheral vascular resistance. In model experiments on dogs, which sustained 20-minute isolated compression ischemia the syndrome of low cardiac output developed too. This indicated the relation of this phenomenon to the disorders in the neuro-humoral regulation of blood circulation.", "contents": "[Pathogenesis of the low cardiac output syndrome in postreanimation states]. Functional reserves of the cardiovascular system during sharp depression of the cardiac output  in the postreanimation period after 15-minute cardiac arrest were studied in experiments on dogs (by loading with different fluid volumes). Acute hypervolemia did not produce any circulatory decompensation. Reinforcement of venous return and changes in the peripheral circulation due to polyglucine loading augmented the CVP temporarily, and produced a stable increase of the AP, of the cardiac output, systolic volume, the work of the left cardiac ventricle and of the total oxygen consumption by the organism. Meanwhile there was a decrease of the peripheral vascular resistance. In model experiments on dogs, which sustained 20-minute isolated compression ischemia the syndrome of low cardiac output developed too. This indicated the relation of this phenomenon to the disorders in the neuro-humoral regulation of blood circulation.", "PMID": 953332}
{"id": "pubmed23n0032_585", "title": "[Toxic and hemolytic properties of serum in the massive transfusion syndrome].", "content": "Toxic and hemolytic properties of the sera of dogs which received massive homologus blood transfusions were studied in experiments on 20 dogs and 432 mice. The toxicity of the recipient's sera was evaluated in vivo on mice with blocked reticulo-endothelial system and by the method of hemocultures. Red cells labeled with radioactive chromium were used to study the hemolytic activity. Investigations carried out demonstrated the acute blood loss wilth the subsequent massive transfusion of the homologous blood to result in the production in the recipient's serum of toxigenic products. These toxigenic properties were determined by two biotesting methods. The maximal toxigenic action was noted in 2 hours and in the course of the 1st day after the substitution. No toxic effect of the sera was found on the 17th day. A parallel study of hemolytic properties of the sera revealed a marked increase of its activity; it was the greatest during the first 24 hours. In three days the hemolytic properties of the serum were no different from the initial one.", "contents": "[Toxic and hemolytic properties of serum in the massive transfusion syndrome]. Toxic and hemolytic properties of the sera of dogs which received massive homologus blood transfusions were studied in experiments on 20 dogs and 432 mice. The toxicity of the recipient's sera was evaluated in vivo on mice with blocked reticulo-endothelial system and by the method of hemocultures. Red cells labeled with radioactive chromium were used to study the hemolytic activity. Investigations carried out demonstrated the acute blood loss wilth the subsequent massive transfusion of the homologous blood to result in the production in the recipient's serum of toxigenic products. These toxigenic properties were determined by two biotesting methods. The maximal toxigenic action was noted in 2 hours and in the course of the 1st day after the substitution. No toxic effect of the sera was found on the 17th day. A parallel study of hemolytic properties of the sera revealed a marked increase of its activity; it was the greatest during the first 24 hours. In three days the hemolytic properties of the serum were no different from the initial one.", "PMID": 953333}
{"id": "pubmed23n0032_586", "title": "[Changes in mesenteric microcirculation in rats following repeated skin burns].", "content": "Acute experiments were conducted on rats; repeated extensive burn of a convalescent who formerly sustained the burn disease was better tolerated, led tono fatal outcome and was accompanied by moderate microcirculatory disturbances. The smae burn was accompanied in intact rats by a severe shock followed by death, intravascular aggregation of erythrocytes and significant microcirculatory disturbances leading to disturbance of tissue nutrition. It is supposed that the results obtained could serve as an indirect proof that toxemia played an important role in the genesis of intravascular aggregation of erythrocytes in burn shock.", "contents": "[Changes in mesenteric microcirculation in rats following repeated skin burns]. Acute experiments were conducted on rats; repeated extensive burn of a convalescent who formerly sustained the burn disease was better tolerated, led tono fatal outcome and was accompanied by moderate microcirculatory disturbances. The smae burn was accompanied in intact rats by a severe shock followed by death, intravascular aggregation of erythrocytes and significant microcirculatory disturbances leading to disturbance of tissue nutrition. It is supposed that the results obtained could serve as an indirect proof that toxemia played an important role in the genesis of intravascular aggregation of erythrocytes in burn shock.", "PMID": 953334}
{"id": "pubmed23n0032_587", "title": "[Mechanism of action of benzene on hematopoiesis (a study of hematopoietic stem cells)].", "content": "A study was made of the effect of benzol on the colony-forming activity in the spleen and the bone marrow of mice in 4 different experimental variants. In benzol hypoplasia of hemopoiesis there was a decrease in the number of the CFU in the hemopoietic organs without any changes in the distribution of their cell types. Incubation of the cells of normal mouse bone marrow with benzol did not induce any decrease of the colony-forming efficiency. Administration of benzol to the lethally irradiated mice after the transplantation of normal bone marrow suspension led to a sharp decrease in the number of CFU with a preponderant depression of the granulocytic colony formation. These data afford to suppose the change in the properties of microenvironment of the hemopoietic stem cells in experimental benzol poisoning.", "contents": "[Mechanism of action of benzene on hematopoiesis (a study of hematopoietic stem cells)]. A study was made of the effect of benzol on the colony-forming activity in the spleen and the bone marrow of mice in 4 different experimental variants. In benzol hypoplasia of hemopoiesis there was a decrease in the number of the CFU in the hemopoietic organs without any changes in the distribution of their cell types. Incubation of the cells of normal mouse bone marrow with benzol did not induce any decrease of the colony-forming efficiency. Administration of benzol to the lethally irradiated mice after the transplantation of normal bone marrow suspension led to a sharp decrease in the number of CFU with a preponderant depression of the granulocytic colony formation. These data afford to suppose the change in the properties of microenvironment of the hemopoietic stem cells in experimental benzol poisoning.", "PMID": 953335}
{"id": "pubmed23n0032_588", "title": "[Effect of contrical on the activity of dehydrogenases and their isoenzymes in the muscles and organs of animals with developing granulation tissue].", "content": "Studies of the influence of contrycal on condition of the enzyme systems of the muscles, liver, kidneys and heart of rats with developing granulation tissue showed contrycal, a protease inhibitor, to have a stimulating effect on the LDH and MDH activity and on their isozyme spectrum. The effect of the inhibitor was expressed in the changes occurring in the state of the enzyme system, both at the site of damage (granulations and the underlying tissue) and in a number of internal organs -- the liver and kidneys.", "contents": "[Effect of contrical on the activity of dehydrogenases and their isoenzymes in the muscles and organs of animals with developing granulation tissue]. Studies of the influence of contrycal on condition of the enzyme systems of the muscles, liver, kidneys and heart of rats with developing granulation tissue showed contrycal, a protease inhibitor, to have a stimulating effect on the LDH and MDH activity and on their isozyme spectrum. The effect of the inhibitor was expressed in the changes occurring in the state of the enzyme system, both at the site of damage (granulations and the underlying tissue) and in a number of internal organs -- the liver and kidneys.", "PMID": 953336}
{"id": "pubmed23n0032_589", "title": "[Effect of training for increased physical loads on the concentration and biosynthesis of ubiquinones in rat skeletal muscle].", "content": "Rats were subjected to a strenuous program of treadmill running for 2.5--3 months. Ubiquinone content was determined in the skeletal muscles and the muscle mitochondria; at the same time incorporation of labeled acetate-1-14C precursor into ubiquinone and sterols by thin muscle slices was studied. Ubiquinone and mitochondrial protein content increased in the muscles of trained rats. Incorporation of 14C into ubiquinone of trained rats increased; as to sterols, their incorporation remained unchanged.", "contents": "[Effect of training for increased physical loads on the concentration and biosynthesis of ubiquinones in rat skeletal muscle]. Rats were subjected to a strenuous program of treadmill running for 2.5--3 months. Ubiquinone content was determined in the skeletal muscles and the muscle mitochondria; at the same time incorporation of labeled acetate-1-14C precursor into ubiquinone and sterols by thin muscle slices was studied. Ubiquinone and mitochondrial protein content increased in the muscles of trained rats. Incorporation of 14C into ubiquinone of trained rats increased; as to sterols, their incorporation remained unchanged.", "PMID": 953337}
{"id": "pubmed23n0032_590", "title": "[Influence of dexamethasone on RNA synthesis in human peripheral blood lymphocytes].", "content": "Biphasic character of dexamethasone action on the lymphoid tissue is observed in the tissue culture of partially purified peripheral blood lymphocytes. Stimulation of the RNA synthesis occurred after 6-hour incubation of lymphocytes with the hormone. Sedimentation analysis of labeled RNA, fractionated in a column containing poly-U sepharose pointed to increase in the quantity of the messenger RNA and enrichment of the cytoplasmic RNA with poly-A sequences. At the same time the Mn++-dependable RNA polymerase sensitive to alpha-amanitin was activated. Inhibition of the RNA synthesis is observed after 24-hour cultivation of lymphocytes with the hormone. Biphasic effect of the steroid is also seen in the reaction of rosetteformation.", "contents": "[Influence of dexamethasone on RNA synthesis in human peripheral blood lymphocytes]. Biphasic character of dexamethasone action on the lymphoid tissue is observed in the tissue culture of partially purified peripheral blood lymphocytes. Stimulation of the RNA synthesis occurred after 6-hour incubation of lymphocytes with the hormone. Sedimentation analysis of labeled RNA, fractionated in a column containing poly-U sepharose pointed to increase in the quantity of the messenger RNA and enrichment of the cytoplasmic RNA with poly-A sequences. At the same time the Mn++-dependable RNA polymerase sensitive to alpha-amanitin was activated. Inhibition of the RNA synthesis is observed after 24-hour cultivation of lymphocytes with the hormone. Biphasic effect of the steroid is also seen in the reaction of rosetteformation.", "PMID": 953338}
{"id": "pubmed23n0032_591", "title": "[Effect of bilateral subdiaphragmatic vagotomy on the activity of several energy metabolism enzymes in the liver].", "content": "Bilateral subdiaphragmatic vagotomy resulted in a significant decrease of hexokinase, glucokinase, glucose-6-phosphoric and 6 phospho-gluconic dehydrogenases, and lactic dehydrogenase activity in the soluble fraction of rat liver. Blood sugar remained unchanged at all the postoperative periods. It is suggested that the above-mentioned alterations in the enzyme activity were caused by the absence of parasympathetic impulses to hepatocytes.", "contents": "[Effect of bilateral subdiaphragmatic vagotomy on the activity of several energy metabolism enzymes in the liver]. Bilateral subdiaphragmatic vagotomy resulted in a significant decrease of hexokinase, glucokinase, glucose-6-phosphoric and 6 phospho-gluconic dehydrogenases, and lactic dehydrogenase activity in the soluble fraction of rat liver. Blood sugar remained unchanged at all the postoperative periods. It is suggested that the above-mentioned alterations in the enzyme activity were caused by the absence of parasympathetic impulses to hepatocytes.", "PMID": 953339}
{"id": "pubmed23n0032_592", "title": "[Effect of a lysosome triton WR 1339 load on distribution of C14-albumin in the livers of rats with chronic hepatitis].", "content": "A possible mechanism of the protective effect of Triton WR1339 during chronic CC14-hepatitis was considered. Supposing that the improvement of the process was due to the intensification of the lysosome heterophage function the authors studied the intensity of the C14-albumin uptake by the liver and its subcellular distribution in the liver of rats in the administration of the detergent to the animals with chronic CCI4-hepatitis, Preliminary administration of the detergent failed to influence the intensity of C14-albumin uptake; subsequent administration of triton WR1339 to rats with toxic hepatitis decrease the protein uptake which reached the values in intact rats. In chronic hepatitis C14-albumin was concentrated in the lysosome fraction. Administration of trition WR1339 to CCI4-treated animals was not accompanied by any coincidence in the peaks of labeled protein and lysosome enzymes. The selective participation of lysosomes of the Kupffer cells providing a more rapid restoration of the liver in chronic hepatitis is discussed.", "contents": "[Effect of a lysosome triton WR 1339 load on distribution of C14-albumin in the livers of rats with chronic hepatitis]. A possible mechanism of the protective effect of Triton WR1339 during chronic CC14-hepatitis was considered. Supposing that the improvement of the process was due to the intensification of the lysosome heterophage function the authors studied the intensity of the C14-albumin uptake by the liver and its subcellular distribution in the liver of rats in the administration of the detergent to the animals with chronic CCI4-hepatitis, Preliminary administration of the detergent failed to influence the intensity of C14-albumin uptake; subsequent administration of triton WR1339 to rats with toxic hepatitis decrease the protein uptake which reached the values in intact rats. In chronic hepatitis C14-albumin was concentrated in the lysosome fraction. Administration of trition WR1339 to CCI4-treated animals was not accompanied by any coincidence in the peaks of labeled protein and lysosome enzymes. The selective participation of lysosomes of the Kupffer cells providing a more rapid restoration of the liver in chronic hepatitis is discussed.", "PMID": 953340}
{"id": "pubmed23n0032_593", "title": "[Effect of hypoxia on intercellular interaction in the myocardium].", "content": "Passive electrical properties of rabbit papillary muscle were studied under normal and hypoxic conditions. The input resistance and the lenght of the electrotonic decay were measured. The results obtained suggested that within a frame-work of a two-dimentional model for anisotropic syncytium the resistance of electrogenic membrane displayed a 1.4-fold decrease on the 10th min after the onset of the hypoxic condition: also the longitudinal (sigma x) and the transverse (sigma y) resistances of the intracellular medium of the syncytium increase 1.39- and 1.3-fold, respectively. An increase in the sigma x and sigma y should be ascribed to the increase in the contact membrane resistance. Derangement of the intercellular interaction under hypoxic conditions is of importance for the understanding of the mechanisms operating in the cardiac arrhythmia due to heart ischemia or myocardial infarction.", "contents": "[Effect of hypoxia on intercellular interaction in the myocardium]. Passive electrical properties of rabbit papillary muscle were studied under normal and hypoxic conditions. The input resistance and the lenght of the electrotonic decay were measured. The results obtained suggested that within a frame-work of a two-dimentional model for anisotropic syncytium the resistance of electrogenic membrane displayed a 1.4-fold decrease on the 10th min after the onset of the hypoxic condition: also the longitudinal (sigma x) and the transverse (sigma y) resistances of the intracellular medium of the syncytium increase 1.39- and 1.3-fold, respectively. An increase in the sigma x and sigma y should be ascribed to the increase in the contact membrane resistance. Derangement of the intercellular interaction under hypoxic conditions is of importance for the understanding of the mechanisms operating in the cardiac arrhythmia due to heart ischemia or myocardial infarction.", "PMID": 953341}
{"id": "pubmed23n0032_594", "title": "[Effect of several tropane derivatives on noradrenaline absorption by hypothalamic synaptic vesicles].", "content": "The influence of some tropan derivatives on the absorption of exogenous norepinephrine was studied in experiments on the isolated synaptic vesicles of the hypothalamus. LK II (beta-morpholinopropionic acid tropine ester dihydrochloride) at the concentration of 1. -5M decreased (like cocaine) the passive consumption of norephinephrine. The dependence of this effect on the transmitter concentration in the incubation medium was demonstrated.", "contents": "[Effect of several tropane derivatives on noradrenaline absorption by hypothalamic synaptic vesicles]. The influence of some tropan derivatives on the absorption of exogenous norepinephrine was studied in experiments on the isolated synaptic vesicles of the hypothalamus. LK II (beta-morpholinopropionic acid tropine ester dihydrochloride) at the concentration of 1. -5M decreased (like cocaine) the passive consumption of norephinephrine. The dependence of this effect on the transmitter concentration in the incubation medium was demonstrated.", "PMID": 953342}
{"id": "pubmed23n0032_595", "title": "[Galanthamine interaction with mouse brain acetylcholinesterase in in vivo experiments].", "content": "Armine treatment of mice against the background of preliminary galanthamine injection diminished the inhibition of the acetylcholinesterase of the brain caused by a reversible inhibitor. This effect was connected with the acetylcholine accumulation and the displacement by it of galanthamine from the active centres of the enzyme. Thus, a competitive character of galanthamine-acetylcholinesterase interaction was shown in vivo.", "contents": "[Galanthamine interaction with mouse brain acetylcholinesterase in in vivo experiments]. Armine treatment of mice against the background of preliminary galanthamine injection diminished the inhibition of the acetylcholinesterase of the brain caused by a reversible inhibitor. This effect was connected with the acetylcholine accumulation and the displacement by it of galanthamine from the active centres of the enzyme. Thus, a competitive character of galanthamine-acetylcholinesterase interaction was shown in vivo.", "PMID": 953343}
{"id": "pubmed23n0032_596", "title": "[Effect of phenformin on fat-carbohydrate metabolism in pregnant rats].", "content": "Rats given phenphormin, an antidiabetic biguanide, from the 1st (8th) day of pregnancy displayed a fall of cholesterol, phospholipids and free fatty acids in the blood; no maldevelopment of the fetus or placenta was seen. Permissibility of prescription of biguanides during pregnancy is discussed.", "contents": "[Effect of phenformin on fat-carbohydrate metabolism in pregnant rats]. Rats given phenphormin, an antidiabetic biguanide, from the 1st (8th) day of pregnancy displayed a fall of cholesterol, phospholipids and free fatty acids in the blood; no maldevelopment of the fetus or placenta was seen. Permissibility of prescription of biguanides during pregnancy is discussed.", "PMID": 953344}
{"id": "pubmed23n0032_597", "title": "[Effect of inhibitors of DNA and protein synthesis on production of mitogenic factor by human lymphocytes stimulated with phytohemagglutinin].", "content": "A study was made of the influence of DNA and protein synthesis inhibitors on the production of mitogenic factor (MF) by human lymphocytes stimulated with phytohemagglutinin. Inhibition of the DNA synthesis with mitomycin failed to decrease MF production but in inhibiting the protein synthesis MF factor production was even increased. A conclusion was drawn that MF preexisted in the lymphoid cell before the beginning of stimulation, not in the ready from but as an inactive precursor; it became activated only in the living cells stimulated with phytohemagglutinin no protein synthesis is required at that moment.", "contents": "[Effect of inhibitors of DNA and protein synthesis on production of mitogenic factor by human lymphocytes stimulated with phytohemagglutinin]. A study was made of the influence of DNA and protein synthesis inhibitors on the production of mitogenic factor (MF) by human lymphocytes stimulated with phytohemagglutinin. Inhibition of the DNA synthesis with mitomycin failed to decrease MF production but in inhibiting the protein synthesis MF factor production was even increased. A conclusion was drawn that MF preexisted in the lymphoid cell before the beginning of stimulation, not in the ready from but as an inactive precursor; it became activated only in the living cells stimulated with phytohemagglutinin no protein synthesis is required at that moment.", "PMID": 953345}
{"id": "pubmed23n0032_598", "title": "[The state of several indices of non-specific immunity in rats with nephrotoxic glomerulonephritis].", "content": "Changes in the interferon reaction of leukocytes, bactericidal property of the blood serum against the E. coli and streptococcus, the activity of beta-lysins in experimental nephrotoxic glomerulonephritis reproducible with the aid of heterogenous nephrotoxic serum in mongrel male rats were studied. This model permitted to trace the changes in the nonspecific protection mechanisms depending on the pathological process in the kidneys. In nephrotoxic glomerulonephritis the most pronounced changes were seen in the interferon reaction of leukocytes: it decreased up to 90% in comparison with the normal depending on the duration of the disease. In difference from this the changes in the anti-bacterial immunity indices were observed at the period of marked nephrotic process activity.", "contents": "[The state of several indices of non-specific immunity in rats with nephrotoxic glomerulonephritis]. Changes in the interferon reaction of leukocytes, bactericidal property of the blood serum against the E. coli and streptococcus, the activity of beta-lysins in experimental nephrotoxic glomerulonephritis reproducible with the aid of heterogenous nephrotoxic serum in mongrel male rats were studied. This model permitted to trace the changes in the nonspecific protection mechanisms depending on the pathological process in the kidneys. In nephrotoxic glomerulonephritis the most pronounced changes were seen in the interferon reaction of leukocytes: it decreased up to 90% in comparison with the normal depending on the duration of the disease. In difference from this the changes in the anti-bacterial immunity indices were observed at the period of marked nephrotic process activity.", "PMID": 953346}
{"id": "pubmed23n0032_599", "title": "[Presence of cross-reacting thymus and brain antigens in the cerebral cortex].", "content": "Rabbit antisera against antigens of mouse, rabbit, guinea pig and human whole brain cross-reacted in the cytotoxic test with the lymphocytes of the thymus, lymph nodes and the spleen of these animal species. Mouse thymocytes were most sensitive to the antibrain sera (cytotoxic index -- 63--100 per cent); cells from other mouse lymphoid organs and lymphocytes of rabbit, guinea pig and man were more resistant. Bone marrow lymphocytes were not damaged by any of these sera. The antigens which induced the cytotoxic properties of the sera were found only in the human cerebral cortex, but not in the white matter of the brain stem.", "contents": "[Presence of cross-reacting thymus and brain antigens in the cerebral cortex]. Rabbit antisera against antigens of mouse, rabbit, guinea pig and human whole brain cross-reacted in the cytotoxic test with the lymphocytes of the thymus, lymph nodes and the spleen of these animal species. Mouse thymocytes were most sensitive to the antibrain sera (cytotoxic index -- 63--100 per cent); cells from other mouse lymphoid organs and lymphocytes of rabbit, guinea pig and man were more resistant. Bone marrow lymphocytes were not damaged by any of these sera. The antigens which induced the cytotoxic properties of the sera were found only in the human cerebral cortex, but not in the white matter of the brain stem.", "PMID": 953347}
{"id": "pubmed23n0032_600", "title": "[Effect of the combined action of rimantadin and dimethyl sulfoxide on phage reproduction].", "content": "Separate and combined action of Dimethylsulfoxid (DMSO) and Rimantadin on the reproduction of phages T--3, T--4, T--7 and lambda was studied. The maximum activation of reproduction of phages T--3 and T--7 by DMSO was observed for 6 minutes of latent period. At the same time this compound did not render the action on reproduction phages T--4 and, because they utilize cell RNA-polimerase. It was shown that DMSO stimulated the synthesis of T--3 phage DNA by action on T--3 phage RNA-polymerase. Combined application of both DMSO and Rimantadin eleminated in some degree its reciprocal effect on T--3 phage macromoelecules synthesis and complet eliminated inhibitory action of Rimantidin on infective phage T--3 production.", "contents": "[Effect of the combined action of rimantadin and dimethyl sulfoxide on phage reproduction]. Separate and combined action of Dimethylsulfoxid (DMSO) and Rimantadin on the reproduction of phages T--3, T--4, T--7 and lambda was studied. The maximum activation of reproduction of phages T--3 and T--7 by DMSO was observed for 6 minutes of latent period. At the same time this compound did not render the action on reproduction phages T--4 and, because they utilize cell RNA-polimerase. It was shown that DMSO stimulated the synthesis of T--3 phage DNA by action on T--3 phage RNA-polymerase. Combined application of both DMSO and Rimantadin eleminated in some degree its reciprocal effect on T--3 phage macromoelecules synthesis and complet eliminated inhibitory action of Rimantidin on infective phage T--3 production.", "PMID": 953348}
{"id": "pubmed23n0032_601", "title": "[Influence of antilymphocyte sera on the development of experimental allergic encephalomyelitis].", "content": "Experimental allergic encephalomyelitis (EAE) was induced in guinea pigs by sensitization of myelinomas of homologous or heterologous (rabbit) brain in the complete Freund's adjuvant. Antilymphocytic serum (ALS) obtained by immunization of rabbits with the lymphocytes of the lymph nodes of guinea pigs was injected subcutaneously for 3--6 days at the early periods before or after the sensitization. ALS produced a marked inhibitory action on the development of the neurological signs of EAE and of histological changes in the form of demyelinization and perivascular cell infiltration in the lumbo-sacral and cervical portions of the spinal cord. Administration of ALS beginning from the 3rd--5th day before the sensitization or from the date of the EAE induction proved to be the most effective. When the injections were started from the 7th or the 12th day after the sensitization an increase in the incidence of the disease occurred in the grooups. A fall of morbidity correlated with a reduction of percentage of positive skin tests in response to the intradermal injection of a homologous myelin. No reduction in the production of the complement-fixing, antibodies to the heterologous antigen was seen in the animals given ALS. This pointed to the participation of cell factors in the development of the pathological process.", "contents": "[Influence of antilymphocyte sera on the development of experimental allergic encephalomyelitis]. Experimental allergic encephalomyelitis (EAE) was induced in guinea pigs by sensitization of myelinomas of homologous or heterologous (rabbit) brain in the complete Freund's adjuvant. Antilymphocytic serum (ALS) obtained by immunization of rabbits with the lymphocytes of the lymph nodes of guinea pigs was injected subcutaneously for 3--6 days at the early periods before or after the sensitization. ALS produced a marked inhibitory action on the development of the neurological signs of EAE and of histological changes in the form of demyelinization and perivascular cell infiltration in the lumbo-sacral and cervical portions of the spinal cord. Administration of ALS beginning from the 3rd--5th day before the sensitization or from the date of the EAE induction proved to be the most effective. When the injections were started from the 7th or the 12th day after the sensitization an increase in the incidence of the disease occurred in the grooups. A fall of morbidity correlated with a reduction of percentage of positive skin tests in response to the intradermal injection of a homologous myelin. No reduction in the production of the complement-fixing, antibodies to the heterologous antigen was seen in the animals given ALS. This pointed to the participation of cell factors in the development of the pathological process.", "PMID": 953349}
{"id": "pubmed23n0032_602", "title": "[Adrenalectomized mice as an experimental model for studying the biological activity of immune complexes].", "content": "Adrenalectomized mice may develop local (skin) and systemic reactions on the introduction of immune complexes formed in vitro. Regularities in respect to the composition of immune complexes (in which their general, toxic, action and the capacity to produce local reactions was expressed) were revealed. Treatment with agents depressing the anaphylactic reaction (cortisone, antihistaminic drugs, anesthetics) diminished the reactions to the immune complex. Local reaction to the immune complex decreased in animals with artificially-induced fall of the blood complement level.", "contents": "[Adrenalectomized mice as an experimental model for studying the biological activity of immune complexes]. Adrenalectomized mice may develop local (skin) and systemic reactions on the introduction of immune complexes formed in vitro. Regularities in respect to the composition of immune complexes (in which their general, toxic, action and the capacity to produce local reactions was expressed) were revealed. Treatment with agents depressing the anaphylactic reaction (cortisone, antihistaminic drugs, anesthetics) diminished the reactions to the immune complex. Local reaction to the immune complex decreased in animals with artificially-induced fall of the blood complement level.", "PMID": 953350}
{"id": "pubmed23n0032_603", "title": "[Detection of an antigen containing 3-hydroxyanthranilic acid in the sera of patients with tumors of different localizations].", "content": "An unusual antigen containing a carcinogenic tryptophane metabolite -- 3-hydroxyanthranylic acid as a hapten (3-HAA-antigen) was found to be present in the blood serum in the overwhelming majority of patients with malignant tumours of various stages and localizations. The 3-HAA-antigen was rarely determined in the patients with nonmalignant diseases was not revealed in the blood serum of healthy donors and patients with benign tumours. The significance of the data obtained for the diagnosis of the tumour process, irrespective of its localization, is discussed.", "contents": "[Detection of an antigen containing 3-hydroxyanthranilic acid in the sera of patients with tumors of different localizations]. An unusual antigen containing a carcinogenic tryptophane metabolite -- 3-hydroxyanthranylic acid as a hapten (3-HAA-antigen) was found to be present in the blood serum in the overwhelming majority of patients with malignant tumours of various stages and localizations. The 3-HAA-antigen was rarely determined in the patients with nonmalignant diseases was not revealed in the blood serum of healthy donors and patients with benign tumours. The significance of the data obtained for the diagnosis of the tumour process, irrespective of its localization, is discussed.", "PMID": 953351}
{"id": "pubmed23n0032_604", "title": "[Circadian rhythms of cell proliferation in mice during the early stages of hepatocarcinogenesis induced by ortho-aminoazotoluol].", "content": "The 24-hour rhythms of the mitotic activity and the changes in the number of DNA-synthesising cells were studied in mouse hepatocytes at the early stages of carcinogenesis of the liver induced with orthoaminoazotoluol in these animals; the autoradiographic method with thymidin-3H was used. During the first stage (that of diffuse irregular hyperplasia) and the second stage (that of focal proliferation) the rhythms of of the changes in the mitotic activity of test animals as incontrols had a distinct monophasic character with the maximum number of mitoses at the early morning hours and the minimum at the evening-night hours. Under normal conditions and during the first stage of carcinogenesis of the liver the DNA-synthesizing cell rhythms had a distinct monophasic character with the maximum during the day and evening hours, respectively; during the second stage of carcinogenesis the rhythm was characterized by the appearance of the second maximum at the early morning hours. The mean 24-hour values of both indices increased during the carcinogenesis.", "contents": "[Circadian rhythms of cell proliferation in mice during the early stages of hepatocarcinogenesis induced by ortho-aminoazotoluol]. The 24-hour rhythms of the mitotic activity and the changes in the number of DNA-synthesising cells were studied in mouse hepatocytes at the early stages of carcinogenesis of the liver induced with orthoaminoazotoluol in these animals; the autoradiographic method with thymidin-3H was used. During the first stage (that of diffuse irregular hyperplasia) and the second stage (that of focal proliferation) the rhythms of of the changes in the mitotic activity of test animals as incontrols had a distinct monophasic character with the maximum number of mitoses at the early morning hours and the minimum at the evening-night hours. Under normal conditions and during the first stage of carcinogenesis of the liver the DNA-synthesizing cell rhythms had a distinct monophasic character with the maximum during the day and evening hours, respectively; during the second stage of carcinogenesis the rhythm was characterized by the appearance of the second maximum at the early morning hours. The mean 24-hour values of both indices increased during the carcinogenesis.", "PMID": 953352}
{"id": "pubmed23n0032_605", "title": "[Influence of antibiotics of the penicillin series on the fetal and newborn development of rats].", "content": "Penicillin injected to rats in high doses at various periods of pregnancy produced no negative effect on the devloping fetus and neonate. At the same time bicillin-3 produced resorption of the embryonic cells and had a negative effect on the heart development.", "contents": "[Influence of antibiotics of the penicillin series on the fetal and newborn development of rats]. Penicillin injected to rats in high doses at various periods of pregnancy produced no negative effect on the devloping fetus and neonate. At the same time bicillin-3 produced resorption of the embryonic cells and had a negative effect on the heart development.", "PMID": 953353}
{"id": "pubmed23n0032_606", "title": "[Effect of cortisone on post-traumatic regeneration of mammalian skeletal muscle].", "content": "Prolonged administration of cortisone to sexually immature rabbits and rats, in which m. tibialis anterior was injured preliminarily, inhibited the process of regeneration of the skeletal muscle tissue; this was expressed in the retarded growth of myosymplasts and of the muscle tubes. By the 15th postoperative day experimental rabbits (in comparison with control) showed a lesser area of muscle component of the regenerated tissue. Analysis of the intensity of methionine-H3 incorporation into the regenerating elements of the muscle tissue demonstrated a significant reduction of the label incorporation into the nuclear and cytoplasmic proteins of the myosymplasts of rats to which cortisone was given. Inhibition of the protein synthesis at the early stage of differentiation of the muscle tissue was expressed to a lesser extent than in the mature differentiated muscle fibers of the intact muscle.", "contents": "[Effect of cortisone on post-traumatic regeneration of mammalian skeletal muscle]. Prolonged administration of cortisone to sexually immature rabbits and rats, in which m. tibialis anterior was injured preliminarily, inhibited the process of regeneration of the skeletal muscle tissue; this was expressed in the retarded growth of myosymplasts and of the muscle tubes. By the 15th postoperative day experimental rabbits (in comparison with control) showed a lesser area of muscle component of the regenerated tissue. Analysis of the intensity of methionine-H3 incorporation into the regenerating elements of the muscle tissue demonstrated a significant reduction of the label incorporation into the nuclear and cytoplasmic proteins of the myosymplasts of rats to which cortisone was given. Inhibition of the protein synthesis at the early stage of differentiation of the muscle tissue was expressed to a lesser extent than in the mature differentiated muscle fibers of the intact muscle.", "PMID": 953354}
{"id": "pubmed23n0032_607", "title": "[Cytologic features of rat liver growth in the embryonal and neonatal periods and during regeneration].", "content": "August rats were used for experiments. The growth of intact embryonal and neonatal liver was compared with the growth of adult regenerating liver at the early periods after partial hepatectomy (22, 48 and 78 hours after resection of 2/3 of the liver). Mitotic activity and the area of hepatocyte nuclei were determined; binucleated cells were counted. There proved to be a number of general regularities in the dynamics of the cytological parameters of growth for the intact embryonal, neonatal and regenerating adult liver of rats at the early periods after the resection.", "contents": "[Cytologic features of rat liver growth in the embryonal and neonatal periods and during regeneration]. August rats were used for experiments. The growth of intact embryonal and neonatal liver was compared with the growth of adult regenerating liver at the early periods after partial hepatectomy (22, 48 and 78 hours after resection of 2/3 of the liver). Mitotic activity and the area of hepatocyte nuclei were determined; binucleated cells were counted. There proved to be a number of general regularities in the dynamics of the cytological parameters of growth for the intact embryonal, neonatal and regenerating adult liver of rats at the early periods after the resection.", "PMID": 953355}
{"id": "pubmed23n0032_608", "title": "[Mechanism of reversibility of colchicine mitosis induced by colcemid].", "content": "Restoration of the normal mitosis after its block by colchicine was due to the additional protein synthesis (reformation of the spindle microtubules). Studies of reversibility of the statmokinetic reaction induced by colcemid showed the block of the protein synthesis by puromycin to have no influence on the rate of restoration of the normal mitotic regimen, but copper ions delayed this process. On the basis of these results it is considered that restoration of the K-mitosis induced by colcemid was mainly due to the tubule reassembly. Probably the prevalence of the processes of reformation and reassembly of microtubules during the restoration depended on the extent of their destruction and the pattern of binding by the damaging agent of the tubular protein pool subunits.", "contents": "[Mechanism of reversibility of colchicine mitosis induced by colcemid]. Restoration of the normal mitosis after its block by colchicine was due to the additional protein synthesis (reformation of the spindle microtubules). Studies of reversibility of the statmokinetic reaction induced by colcemid showed the block of the protein synthesis by puromycin to have no influence on the rate of restoration of the normal mitotic regimen, but copper ions delayed this process. On the basis of these results it is considered that restoration of the K-mitosis induced by colcemid was mainly due to the tubule reassembly. Probably the prevalence of the processes of reformation and reassembly of microtubules during the restoration depended on the extent of their destruction and the pattern of binding by the damaging agent of the tubular protein pool subunits.", "PMID": 953356}
{"id": "pubmed23n0032_609", "title": "[Electron-microscopic radioautography of DNA-synthesizing cells of growing rat smooth muscle tissue].", "content": "DNA-synthesizing cells of rat stomach muscle tissue following 50% resection of the fundal part, identical cells of the vena cava after disturbance of the blood out flow, and of the appendix on contraction of the ascending part of the large intestine were studied by electron microscopic autoradiography. DNA synthesis in the differentiated myocyte nuclei of the stomach muscle tissue, and of the \"activated\" myocytes of the stomach and the vein was observed. There were signs of asynchronous DNA synthesis in the nuclei of some smooth muscle cells and fibroblasts.", "contents": "[Electron-microscopic radioautography of DNA-synthesizing cells of growing rat smooth muscle tissue]. DNA-synthesizing cells of rat stomach muscle tissue following 50% resection of the fundal part, identical cells of the vena cava after disturbance of the blood out flow, and of the appendix on contraction of the ascending part of the large intestine were studied by electron microscopic autoradiography. DNA synthesis in the differentiated myocyte nuclei of the stomach muscle tissue, and of the \"activated\" myocytes of the stomach and the vein was observed. There were signs of asynchronous DNA synthesis in the nuclei of some smooth muscle cells and fibroblasts.", "PMID": 953357}
{"id": "pubmed23n0032_610", "title": "[Post-traumatic regeneration of the epithelium of the gastric and intestinal mucous membranes of dogs].", "content": "The work was devoted to the study of the sources of reparative regeneration of the epithelium of the gastric and intestinal mucosa. A number of surgical interventions on the stomach and intestine of 1 dogs were performed for the solution of this problem. On the basis of the investigations carried out the leading role in the regeneration of the gastric and intestinal mucosa was found to be played by uninjured epithelium surrounding the area of the surgical intervention. This was confirmed by the fact that a mucosa-free intestinal pedicle graft implanted into the defect of the wall of the stomach was covered by gastric mucosa; a stomach graft devoid of own mucosa became covered with intestinal mucosa when displaced in the form of a cylindrical \"insertion\" into the intestinal tube; mucosa-free stomach pouch became obliterated; this could be reliably attributed to the absence of the main source of regeneration -- uninjured mucosal epithelium along the periphery of the defect. No regeneration of the epithelium of the gastric mucosa from the implanted cells was revealed; this served as an additional confirmation of the fact that regeneration under conditions of the mentioned experiments occurred on account of creeping of epithelial cells surrounding the area of the surgical intervention over the free surface of mucosa.", "contents": "[Post-traumatic regeneration of the epithelium of the gastric and intestinal mucous membranes of dogs]. The work was devoted to the study of the sources of reparative regeneration of the epithelium of the gastric and intestinal mucosa. A number of surgical interventions on the stomach and intestine of 1 dogs were performed for the solution of this problem. On the basis of the investigations carried out the leading role in the regeneration of the gastric and intestinal mucosa was found to be played by uninjured epithelium surrounding the area of the surgical intervention. This was confirmed by the fact that a mucosa-free intestinal pedicle graft implanted into the defect of the wall of the stomach was covered by gastric mucosa; a stomach graft devoid of own mucosa became covered with intestinal mucosa when displaced in the form of a cylindrical \"insertion\" into the intestinal tube; mucosa-free stomach pouch became obliterated; this could be reliably attributed to the absence of the main source of regeneration -- uninjured mucosal epithelium along the periphery of the defect. No regeneration of the epithelium of the gastric mucosa from the implanted cells was revealed; this served as an additional confirmation of the fact that regeneration under conditions of the mentioned experiments occurred on account of creeping of epithelial cells surrounding the area of the surgical intervention over the free surface of mucosa.", "PMID": 953358}
{"id": "pubmed23n0032_611", "title": "[A method of studying hydrochloric acid secretion in isolated gastric mucous membrane using a two-channel pH-meter].", "content": "A new method of hydrochloric acid secretion recording on two symmetrical parts of the same isolated gastric mucosa is described. A two-channel system which had a number of important advantages over a single-channel system was used. The advantage of this method lay in a possibility of a more exact differentiation of the influence of different substances on the rate of H+ secretion and in a considerable decrease in the number of animals required for the experiments. The apparatus scheme for a two-channel recording of H+ secretion, and design of the four-chamber cuvette are described.", "contents": "[A method of studying hydrochloric acid secretion in isolated gastric mucous membrane using a two-channel pH-meter]. A new method of hydrochloric acid secretion recording on two symmetrical parts of the same isolated gastric mucosa is described. A two-channel system which had a number of important advantages over a single-channel system was used. The advantage of this method lay in a possibility of a more exact differentiation of the influence of different substances on the rate of H+ secretion and in a considerable decrease in the number of animals required for the experiments. The apparatus scheme for a two-channel recording of H+ secretion, and design of the four-chamber cuvette are described.", "PMID": 953359}
{"id": "pubmed23n0032_612", "title": "[A rapid method standardizing cholagogue substances on mice].", "content": "The authors described a rapid method of selection and quantitative assessment of the specific activity of substances (insulin, chologon and mebethizol: 2-mercaptobenzthiazol with a cholagogue action of mice. The gall bladder obtained from the animals was weighed with and wihtout the bile; it was thus possible to determine the amount of bile accumulated in the gall bladder under the action of the agents under study during a definite period of time. The method described may be used for pharmacological screening.", "contents": "[A rapid method standardizing cholagogue substances on mice]. The authors described a rapid method of selection and quantitative assessment of the specific activity of substances (insulin, chologon and mebethizol: 2-mercaptobenzthiazol with a cholagogue action of mice. The gall bladder obtained from the animals was weighed with and wihtout the bile; it was thus possible to determine the amount of bile accumulated in the gall bladder under the action of the agents under study during a definite period of time. The method described may be used for pharmacological screening.", "PMID": 953360}
{"id": "pubmed23n0032_613", "title": "Human marrow erythropoiesis in culture. I. Characterization of methylcellulose colony assay.", "content": "We examined the morphological and functional characteristics of human marrow erythrocytes cultured with a recently developed methylcellulose colony assay technique. Erythrocytic cells in various stages of development were observed, and a significant degree of maturational synchrony within individual colonies was noted. By light microscopy, colonies consisting of late normoblasts appeared compact, had an orange hue attributable to their hemoglobin, and demonstrated pseudoperoxidase activity, whereas colonies composed of early erythroblasts grew less compact or in clusters of smaller cell aggregates and showed no reddish tinge. Colonies possessing intermediate features were also observed. Maturational synchrony of individual colonies was confirmed using ransmission and scanning electron microscopy. The ultrastructure and cytochemistry of most immature cells were normal. The mature erythrocytes, however, were severely microcytic and hypochromic and contained one to several Heinz bodies. These defects in the cytoplasmic maturation of erythrocytes corresponded with impaired granulocytic maturation in culture, which we observed previously, and suggest environmental or nutritional defects in culture. Linearity of the method was confirmed using five normal bone marrows. Erythropoietin dose-responses observed in ten normal marrows were comparable to the previously reported results and revealed significant variation in individual plating efficiencies.", "contents": "Human marrow erythropoiesis in culture. I. Characterization of methylcellulose colony assay. We examined the morphological and functional characteristics of human marrow erythrocytes cultured with a recently developed methylcellulose colony assay technique. Erythrocytic cells in various stages of development were observed, and a significant degree of maturational synchrony within individual colonies was noted. By light microscopy, colonies consisting of late normoblasts appeared compact, had an orange hue attributable to their hemoglobin, and demonstrated pseudoperoxidase activity, whereas colonies composed of early erythroblasts grew less compact or in clusters of smaller cell aggregates and showed no reddish tinge. Colonies possessing intermediate features were also observed. Maturational synchrony of individual colonies was confirmed using ransmission and scanning electron microscopy. The ultrastructure and cytochemistry of most immature cells were normal. The mature erythrocytes, however, were severely microcytic and hypochromic and contained one to several Heinz bodies. These defects in the cytoplasmic maturation of erythrocytes corresponded with impaired granulocytic maturation in culture, which we observed previously, and suggest environmental or nutritional defects in culture. Linearity of the method was confirmed using five normal bone marrows. Erythropoietin dose-responses observed in ten normal marrows were comparable to the previously reported results and revealed significant variation in individual plating efficiencies.", "PMID": 953362}
{"id": "pubmed23n0032_614", "title": "Erythropoietic events in cultured embryonic mouse spleen.", "content": "The events of erythropoiesis in cultured embryonic mouse spleens isolated on the 12th day of gestation were compared with those occurring in spleens of corresponding gestational days in vivo. It was found that the development of erythroid precursors in the cultured spleen depended on the presence of erythropoietin in the culture medium. A progressive increase in the number of erythroid precursors, with a marked shift to mature cells, was observed in spleens cultured with erythropoietin. Although the origin of the hemocytoblasts in the spleen isolated in the earliest stage of its development remains obscure, the present study provided evidence that erythropoiesis in the embryonic mouse spleen was indigenous to this organ and did not result from colonization of erythroid precursors originating in the liver.", "contents": "Erythropoietic events in cultured embryonic mouse spleen. The events of erythropoiesis in cultured embryonic mouse spleens isolated on the 12th day of gestation were compared with those occurring in spleens of corresponding gestational days in vivo. It was found that the development of erythroid precursors in the cultured spleen depended on the presence of erythropoietin in the culture medium. A progressive increase in the number of erythroid precursors, with a marked shift to mature cells, was observed in spleens cultured with erythropoietin. Although the origin of the hemocytoblasts in the spleen isolated in the earliest stage of its development remains obscure, the present study provided evidence that erythropoiesis in the embryonic mouse spleen was indigenous to this organ and did not result from colonization of erythroid precursors originating in the liver.", "PMID": 953363}
{"id": "pubmed23n0032_615", "title": "Factors which affect erythropoiesis in partially nephrectomized and sham-operated rats.", "content": "Current concepts of the pathogenesis of anemia in uremic animals are derived mainly from the results of studies performed either in vitro or in bilaterally nephrectomized animals. These data may not be applicable to the situation which exists in more chronically uremic animals. In 1932, Chanutin and Ferris showed that removal of five-sixths of the renal mass caused rats to become uremic and to remain so for a prolonged period of time. Rats made uremic in this manner were utilized as models for studying the pathogenesis of the anemia of uremia. Removeal of five-sixths of the renal mass of rats caused their BUNs to rise to over 100 mg/100 ml and to remain at this level for over 3 wk. The hematocrits of these uremic rats fell from 42% to approximately 30% in 3 wk. Erythropoietin (Ep) production immediately fell to a barely detectable level postoperatively and did not increase significantly in 3 wk, although the renal remnant hypertrophied. Extrarenal Ep production also remained at a low level and did not increase during the 3-wk observation period. The response of plethoric uremic rats to 2 units of Ep was as great (in some experiments greater) as that of sham-operated ones. A surprising finding was that plethoric uremic rats, injected with saline rather than with Ep, incorporated more 59Fe into their red blood cells than did sham-operated ones. This finding suggested that in uremic rats erythropoiesis was less markedly suppressed by plethora than it was in non-uremic rats.", "contents": "Factors which affect erythropoiesis in partially nephrectomized and sham-operated rats. Current concepts of the pathogenesis of anemia in uremic animals are derived mainly from the results of studies performed either in vitro or in bilaterally nephrectomized animals. These data may not be applicable to the situation which exists in more chronically uremic animals. In 1932, Chanutin and Ferris showed that removal of five-sixths of the renal mass caused rats to become uremic and to remain so for a prolonged period of time. Rats made uremic in this manner were utilized as models for studying the pathogenesis of the anemia of uremia. Removeal of five-sixths of the renal mass of rats caused their BUNs to rise to over 100 mg/100 ml and to remain at this level for over 3 wk. The hematocrits of these uremic rats fell from 42% to approximately 30% in 3 wk. Erythropoietin (Ep) production immediately fell to a barely detectable level postoperatively and did not increase significantly in 3 wk, although the renal remnant hypertrophied. Extrarenal Ep production also remained at a low level and did not increase during the 3-wk observation period. The response of plethoric uremic rats to 2 units of Ep was as great (in some experiments greater) as that of sham-operated ones. A surprising finding was that plethoric uremic rats, injected with saline rather than with Ep, incorporated more 59Fe into their red blood cells than did sham-operated ones. This finding suggested that in uremic rats erythropoiesis was less markedly suppressed by plethora than it was in non-uremic rats.", "PMID": 953364}
{"id": "pubmed23n0032_616", "title": "Evaluation of the iron status of a population.", "content": "The iron status of a population of 1564 subjects living in the northwestern United States was evaluated by measurements of transferrin saturation, red cell protoporphyrin, and serum ferritin. The frequency distribution of these parameters showed no distinct separation between normal and iron-deficient subjects. When only one of these three parameters was abnormal (transferrin saturation below 15%, red cell protoporphyrin above 100 mug/ml packed red blood cells, serum ferritin below 12 ng/ml), the prevalence of anemia was only slightly greater (10.9%) than in the entire sample (8.3%). The prevalence of anemia was increased to 28% in individuals with two or more abnormal parameters, and to 63% when all three parameters were abnormal. As defined by the presence of at least two abnormal parameters, the prevalence of iron deficiency in various populations separated on the basis of age and sex ranged from 3% in adolescent and adult males to 20% in menstruating women. It is concluded that the accuracy of detecting iron deficiency in population surveys can be substantially improved by employing a battery of laboratory measurements of the iron status.", "contents": "Evaluation of the iron status of a population. The iron status of a population of 1564 subjects living in the northwestern United States was evaluated by measurements of transferrin saturation, red cell protoporphyrin, and serum ferritin. The frequency distribution of these parameters showed no distinct separation between normal and iron-deficient subjects. When only one of these three parameters was abnormal (transferrin saturation below 15%, red cell protoporphyrin above 100 mug/ml packed red blood cells, serum ferritin below 12 ng/ml), the prevalence of anemia was only slightly greater (10.9%) than in the entire sample (8.3%). The prevalence of anemia was increased to 28% in individuals with two or more abnormal parameters, and to 63% when all three parameters were abnormal. As defined by the presence of at least two abnormal parameters, the prevalence of iron deficiency in various populations separated on the basis of age and sex ranged from 3% in adolescent and adult males to 20% in menstruating women. It is concluded that the accuracy of detecting iron deficiency in population surveys can be substantially improved by employing a battery of laboratory measurements of the iron status.", "PMID": 953365}
{"id": "pubmed23n0032_617", "title": "Distribution of spermidine and spermine in blood from cystic fibrosis patients and control subjects.", "content": "Previous studies have shown an abnormality of the spermidine-to-spermine (Spd/Spm) ratio in whole blood of cystic fibrosis homo-and heterozygotes. To investigate Spd and Spm distribution amoung blood components as a possible cause of the abnormality, blood was fractionated using Rabinowitz's glass bead technique and Boyum's Ficoll-Hypaque method. Free (unconjugated) polyamines were  extracted with perchloric acid and quantitated on an amino acid analyzer. In controls, mean +/- SEM concentrations in nmoles/10(9) cells of Spd and Spm, respectively, were 1.02 +/- 0.08 and 0.894 +/- 0.28 for erythrocytes; 126 +/- 31 and 357 +/- 105 for lymphocytes; 36 +/- 16 and 240 +/- 33 for granulocytes; and less than 0.5 and less than 0.5 nmoles/ml for plasma. When converted to the concentration in whole blood, it was found that greater than 90% of Spd and over 70% of Spm was associated with erythrocytes. While the higher cellular concentration in leukocytes was not unexpected, the fact that Spd and Spm in whole blood were primarily associated with erythrocytes was a new finding. Comparison with controls revealed that the Spd/Spm ratio in both whole blood and erythrocytes was significantly higher in the group of cystic fibrosis patients.", "contents": "Distribution of spermidine and spermine in blood from cystic fibrosis patients and control subjects. Previous studies have shown an abnormality of the spermidine-to-spermine (Spd/Spm) ratio in whole blood of cystic fibrosis homo-and heterozygotes. To investigate Spd and Spm distribution amoung blood components as a possible cause of the abnormality, blood was fractionated using Rabinowitz's glass bead technique and Boyum's Ficoll-Hypaque method. Free (unconjugated) polyamines were  extracted with perchloric acid and quantitated on an amino acid analyzer. In controls, mean +/- SEM concentrations in nmoles/10(9) cells of Spd and Spm, respectively, were 1.02 +/- 0.08 and 0.894 +/- 0.28 for erythrocytes; 126 +/- 31 and 357 +/- 105 for lymphocytes; 36 +/- 16 and 240 +/- 33 for granulocytes; and less than 0.5 and less than 0.5 nmoles/ml for plasma. When converted to the concentration in whole blood, it was found that greater than 90% of Spd and over 70% of Spm was associated with erythrocytes. While the higher cellular concentration in leukocytes was not unexpected, the fact that Spd and Spm in whole blood were primarily associated with erythrocytes was a new finding. Comparison with controls revealed that the Spd/Spm ratio in both whole blood and erythrocytes was significantly higher in the group of cystic fibrosis patients.", "PMID": 953366}
{"id": "pubmed23n0032_618", "title": "Peptic ulceration in men. Epidemiology and medical care.", "content": "Estimates of the population prevalence of peptic and duodenal ulceration in men aged between 25 and 64 years were made in the London borough of Lambeth. The sampling frame for these estimates was a 20% private census. The lifetime prevalence rate of proved peptic ulcer (haematemesis, gastric and duodenal ulcers as validated by operation or barium meal), adjusted for age and social class, was estimated to be 6-7%, while the similarly adjusted lifetime prevalence for duodenal ulcer was 4-4%. The lifetime prevalences increased with age but not significantly so. A social class gradient was demonstrated with the highest prevalence in social class I and II. Previously described associations with blood group, secretor status, and serum pepsinogen were confirmed. Reported use of medical services increased with increasing severity of symptoms. A large number of respondents, however, who reported symptoms reported no medical care. It seemed unlikely that those men who reported symptoms and no medical care had demonstrable peptic ulcers.", "contents": "Peptic ulceration in men. Epidemiology and medical care. Estimates of the population prevalence of peptic and duodenal ulceration in men aged between 25 and 64 years were made in the London borough of Lambeth. The sampling frame for these estimates was a 20% private census. The lifetime prevalence rate of proved peptic ulcer (haematemesis, gastric and duodenal ulcers as validated by operation or barium meal), adjusted for age and social class, was estimated to be 6-7%, while the similarly adjusted lifetime prevalence for duodenal ulcer was 4-4%. The lifetime prevalences increased with age but not significantly so. A social class gradient was demonstrated with the highest prevalence in social class I and II. Previously described associations with blood group, secretor status, and serum pepsinogen were confirmed. Reported use of medical services increased with increasing severity of symptoms. A large number of respondents, however, who reported symptoms reported no medical care. It seemed unlikely that those men who reported symptoms and no medical care had demonstrable peptic ulcers.", "PMID": 953375}
{"id": "pubmed23n0032_619", "title": "Personality scores and smoking behaviour. A longitudinal study.", "content": "The personality scores at 16 years of age of 2753 people, all members of the National Survey of Health and Development, were related, in a follow-up study, to cigarette smoking behaviour in their young adult years. Survey members who recorded high neuroticism scores were found to be more likely to smoke than those with low scores and, among the smokers, deep inhalers formed the most neurotic group. Extraverts were more likely to smoke than introverts, the mean extraversion score being greatest for the male smokers with a high daily consumption of cigarettes. The personality scores were found to have some power in predicting changes in smoking behaviour. Neurotics and extraverts who had not started to smoke by the time of completing the personality inventory at 16 were more likely than the stable and introverted to take up the habit subsequently. Among survey members who were regular smokers at the time of completing the personality inventory the proportion giving up smoking by the time they reached the age of 25 years was related to consumption level recorded at 20 years and the personality scores recorded at 16, stable extraverts among the men being most likely to stop smoking.", "contents": "Personality scores and smoking behaviour. A longitudinal study. The personality scores at 16 years of age of 2753 people, all members of the National Survey of Health and Development, were related, in a follow-up study, to cigarette smoking behaviour in their young adult years. Survey members who recorded high neuroticism scores were found to be more likely to smoke than those with low scores and, among the smokers, deep inhalers formed the most neurotic group. Extraverts were more likely to smoke than introverts, the mean extraversion score being greatest for the male smokers with a high daily consumption of cigarettes. The personality scores were found to have some power in predicting changes in smoking behaviour. Neurotics and extraverts who had not started to smoke by the time of completing the personality inventory at 16 were more likely than the stable and introverted to take up the habit subsequently. Among survey members who were regular smokers at the time of completing the personality inventory the proportion giving up smoking by the time they reached the age of 25 years was related to consumption level recorded at 20 years and the personality scores recorded at 16, stable extraverts among the men being most likely to stop smoking.", "PMID": 953376}
{"id": "pubmed23n0032_620", "title": "Anencephalus in Scotland 1961-72.", "content": "Data relating to the incidence of anencephalus for the 12-year period 1961-72 were abstracted from the Statistical Reviews of the Registrar General for Scotland. It was shown that considerable geographical variation is still apparent with the highlands having, in general, the lower incidences. In comparison with the earlier study of Edwards (1958), there were some changes: the incidence in the areas to the west had increased and that in those to the east decreased. During the 12-year period there was an overall decline in the incidence of the lesion; this was most marked in births to women under 20 years, and to those of social classes III, IV, and V. The decline was least apparent for births to women of high social class and the unmarried. It was shown that there was little seasonal variation in the time of delivery, but that even when the trend had been taken into account the yearly fluctuation was significantly different from that expected, with an excess of cases in 1961 and 1971.", "contents": "Anencephalus in Scotland 1961-72. Data relating to the incidence of anencephalus for the 12-year period 1961-72 were abstracted from the Statistical Reviews of the Registrar General for Scotland. It was shown that considerable geographical variation is still apparent with the highlands having, in general, the lower incidences. In comparison with the earlier study of Edwards (1958), there were some changes: the incidence in the areas to the west had increased and that in those to the east decreased. During the 12-year period there was an overall decline in the incidence of the lesion; this was most marked in births to women under 20 years, and to those of social classes III, IV, and V. The decline was least apparent for births to women of high social class and the unmarried. It was shown that there was little seasonal variation in the time of delivery, but that even when the trend had been taken into account the yearly fluctuation was significantly different from that expected, with an excess of cases in 1961 and 1971.", "PMID": 953377}
{"id": "pubmed23n0032_621", "title": "Cancer in the offspring of fathers in hydrocarbon-related occupations.", "content": "A case-control study has been conducted to see whether a hydrocarbon-related occupation of the father at the time of conception constitutes a risk factor for malignant disease in the offspring. The series comprised 852 cancer cases from the Finnish Cancer Registry and 852 controls matched for date of birth and domicile. The father's occupation for both the cases and controls was ascertained from the records of antenatal clinics. No significant associations were found between the commonest types of childhood cancer and hydrocarbon-related occupations--that is, motor-vehicle mechanics, machinists, miners, painters, and motor-vehicle drivers. Risk ratio 2 was excluded from most of the 95% confidence intervals for children under 15 years of age. The results do not support the hypothesis that there is an excess risk of cancer in the children of fathers in hydrocarbon-related occupations.", "contents": "Cancer in the offspring of fathers in hydrocarbon-related occupations. A case-control study has been conducted to see whether a hydrocarbon-related occupation of the father at the time of conception constitutes a risk factor for malignant disease in the offspring. The series comprised 852 cancer cases from the Finnish Cancer Registry and 852 controls matched for date of birth and domicile. The father's occupation for both the cases and controls was ascertained from the records of antenatal clinics. No significant associations were found between the commonest types of childhood cancer and hydrocarbon-related occupations--that is, motor-vehicle mechanics, machinists, miners, painters, and motor-vehicle drivers. Risk ratio 2 was excluded from most of the 95% confidence intervals for children under 15 years of age. The results do not support the hypothesis that there is an excess risk of cancer in the children of fathers in hydrocarbon-related occupations.", "PMID": 953378}
{"id": "pubmed23n0032_622", "title": "Disability: a model and measurement technique.", "content": "Current methods of ranking or scoring disability tend to be arbitrary. A new method is put forward on the hypothesis that disability progresses in regular, cumulative patterns. A model of disability is defined and tested with the use of Guttman scale analysis. Its validity is indicated on data from a survey in the community and from postsurgical patients, and some factors involved in scale variation are identified. The model provides a simple measurement technique and has implications for the assessment of individual disadvantage, for the prediction of progress in recovery or deterioration, and for evaluation of the outcome of treatment regimes.", "contents": "Disability: a model and measurement technique. Current methods of ranking or scoring disability tend to be arbitrary. A new method is put forward on the hypothesis that disability progresses in regular, cumulative patterns. A model of disability is defined and tested with the use of Guttman scale analysis. Its validity is indicated on data from a survey in the community and from postsurgical patients, and some factors involved in scale variation are identified. The model provides a simple measurement technique and has implications for the assessment of individual disadvantage, for the prediction of progress in recovery or deterioration, and for evaluation of the outcome of treatment regimes.", "PMID": 953379}
{"id": "pubmed23n0032_623", "title": "The needs of old people. The 'interval' as a method of measurement.", "content": "A survey was conducted on 1035 persons constituting a semi-random age-structured sample of the populations of three areas in the west of Scotland. The object was to attempt to estimate the needs of this population for domiciliary services. A method was developed of classifying the subjects into 12 'care groups' based on two characteristics, those of 'potential need' and 'solitude'. 'Potential need' was a measure of disability and its severity was graded according to the intervals that elapsed between necessary periods of help. 'Solitude' was a measure of the time during each day when potential sources of help were spontaneously available. 'Potential need' was found to be related linearly to age but to be independent of the other variables studied. 'Solitude' was commonest in the 75-84-year age group and varied in the three areas studied, but no relationship with any other variable was detected. An estimate was made of the domiciliary services which would be required to ensure a satisfactory standard of care for the subjects in the sample. Assuming present or slightly better levels of hospital provision and slightly lower provision of residential homes there is still a need for a very great increase in domiciliary services if satisfactory standards of community care are to be attained.", "contents": "The needs of old people. The 'interval' as a method of measurement. A survey was conducted on 1035 persons constituting a semi-random age-structured sample of the populations of three areas in the west of Scotland. The object was to attempt to estimate the needs of this population for domiciliary services. A method was developed of classifying the subjects into 12 'care groups' based on two characteristics, those of 'potential need' and 'solitude'. 'Potential need' was a measure of disability and its severity was graded according to the intervals that elapsed between necessary periods of help. 'Solitude' was a measure of the time during each day when potential sources of help were spontaneously available. 'Potential need' was found to be related linearly to age but to be independent of the other variables studied. 'Solitude' was commonest in the 75-84-year age group and varied in the three areas studied, but no relationship with any other variable was detected. An estimate was made of the domiciliary services which would be required to ensure a satisfactory standard of care for the subjects in the sample. Assuming present or slightly better levels of hospital provision and slightly lower provision of residential homes there is still a need for a very great increase in domiciliary services if satisfactory standards of community care are to be attained.", "PMID": 953380}
{"id": "pubmed23n0032_624", "title": "The coal gas story. United Kingdom suicide rates, 1960-71.", "content": "A detailed analysis of suicide rates between 1960 and 1971 for England and Wales and for Scotland confirms that all age-sex subgroups have shown a marked decline in suicide due to domestic gas, corresponding in time to the fall in the CO content. After considering data on the effects of the International Classification of Diseases (ICD) Eighth Revision, accident mortality, some personal characteristics of coal gas suicides, and the use of coal gas in parasuicide it was concluded that a simple casual explantation was likely. Suicide due to non-gas methods has in general increased, markedly so in some groups. It was suggested that neither improved psychiatric services nor voluntary agencies could have produced such changes. The 'compensatory' trend of gas and non-gas suicide rates was indicated for certain age-sex subgroups. The continuing need for suicide research was pointed out, and questions were raised concerning the psychological meaning of the epidemiological data.", "contents": "The coal gas story. United Kingdom suicide rates, 1960-71. A detailed analysis of suicide rates between 1960 and 1971 for England and Wales and for Scotland confirms that all age-sex subgroups have shown a marked decline in suicide due to domestic gas, corresponding in time to the fall in the CO content. After considering data on the effects of the International Classification of Diseases (ICD) Eighth Revision, accident mortality, some personal characteristics of coal gas suicides, and the use of coal gas in parasuicide it was concluded that a simple casual explantation was likely. Suicide due to non-gas methods has in general increased, markedly so in some groups. It was suggested that neither improved psychiatric services nor voluntary agencies could have produced such changes. The 'compensatory' trend of gas and non-gas suicide rates was indicated for certain age-sex subgroups. The continuing need for suicide research was pointed out, and questions were raised concerning the psychological meaning of the epidemiological data.", "PMID": 953381}
{"id": "pubmed23n0032_625", "title": "Risk factors for myocardial infarction in young women.", "content": "Seventy-seven women discharged from hospital with a diagnosis of myocardial infarction and 207 control patients were investigated. All were under 45 years of age at the time of admission. Heavy cigarette smoking, reported treatment for pre-eclamptic toxaemia, and type II hyperlipoproteinaemia were found to be independent risk factors for myocardial infarction. Reported treatment for hypertension and diabetes are probably also independently associated with subsequent development of the condition, but the associations between myocardial infarction and reported treatment for obesity and psychiatric illness appear to be secondary. Previous publications have suggested that use of oral contraceptives is an independent risk factor. Examination of the effect of several factors combined, suggests that they act synergistically, the presence of three or more factors increasing the risk 128-fold.", "contents": "Risk factors for myocardial infarction in young women. Seventy-seven women discharged from hospital with a diagnosis of myocardial infarction and 207 control patients were investigated. All were under 45 years of age at the time of admission. Heavy cigarette smoking, reported treatment for pre-eclamptic toxaemia, and type II hyperlipoproteinaemia were found to be independent risk factors for myocardial infarction. Reported treatment for hypertension and diabetes are probably also independently associated with subsequent development of the condition, but the associations between myocardial infarction and reported treatment for obesity and psychiatric illness appear to be secondary. Previous publications have suggested that use of oral contraceptives is an independent risk factor. Examination of the effect of several factors combined, suggests that they act synergistically, the presence of three or more factors increasing the risk 128-fold.", "PMID": 953382}
{"id": "pubmed23n0032_626", "title": "The role of small bowel follow-through examination in the diagnosis of coeliac disease.", "content": "A retrospective study of 105 barium follow-through examinations on 102 patients suspected of having malabsorption syndrome has been carried out to assess the usefulness of this technique in the diagnosis of coeliac disease. Comparison with histological and biochemical data has been made. Of the 34 examinations on 31 patients with a proven diagnosis of coeliac disease, 30 examinations showed both abnormal X-ray and biopsy findings, two examinations showed normal X-ray appearance but abnormal jejunal biopsy and two showed normal jejunal histology but abnormal X-ray findings. There were two cases with sensitivity, non-invasive nature, ease and cheapness of the technique, barium follow-through examination is suggested as the initial investigation in patients suspected of coeliac disease.", "contents": "The role of small bowel follow-through examination in the diagnosis of coeliac disease. A retrospective study of 105 barium follow-through examinations on 102 patients suspected of having malabsorption syndrome has been carried out to assess the usefulness of this technique in the diagnosis of coeliac disease. Comparison with histological and biochemical data has been made. Of the 34 examinations on 31 patients with a proven diagnosis of coeliac disease, 30 examinations showed both abnormal X-ray and biopsy findings, two examinations showed normal X-ray appearance but abnormal jejunal biopsy and two showed normal jejunal histology but abnormal X-ray findings. There were two cases with sensitivity, non-invasive nature, ease and cheapness of the technique, barium follow-through examination is suggested as the initial investigation in patients suspected of coeliac disease.", "PMID": 953384}
{"id": "pubmed23n0032_627", "title": "Azygography in the pre-treatment evaluation of oesophageal carcinoma.", "content": "Azygography is a useful technique for the pre-operative detection of unresectability of oesophageal malignancies. Invarison of the azygos vein by oesophageal carcinoma occurs because of the anatomic proximity of the thoracic oesophagus and the azygos vein. Azygography may be performed by either intraosseous injection of a rib or by direct retrograde catheterization. Complete obstruction of the azygos vein indicates that an oesophageal carcinoma is unresectable if no other intrathoracic disease is evident.", "contents": "Azygography in the pre-treatment evaluation of oesophageal carcinoma. Azygography is a useful technique for the pre-operative detection of unresectability of oesophageal malignancies. Invarison of the azygos vein by oesophageal carcinoma occurs because of the anatomic proximity of the thoracic oesophagus and the azygos vein. Azygography may be performed by either intraosseous injection of a rib or by direct retrograde catheterization. Complete obstruction of the azygos vein indicates that an oesophageal carcinoma is unresectable if no other intrathoracic disease is evident.", "PMID": 953385}
{"id": "pubmed23n0032_628", "title": "The problem of discrimination in mammography. Arguments for using a biological test object.", "content": "A specimen of breast tissue containing a carcinoma with multiple calcifications was used as a test object and examined by a range of mammographic techniques. The images were compared with a photograph of the actual specimen which was cleared and stained for calcium salts. A quantitative method of evaluating the discriminating ability of each technique is described and used to compare these images. Xeroradiography and Kodak Crystallex film clearly excelled other imaging methods. The value of using a biological test object is discussed.", "contents": "The problem of discrimination in mammography. Arguments for using a biological test object. A specimen of breast tissue containing a carcinoma with multiple calcifications was used as a test object and examined by a range of mammographic techniques. The images were compared with a photograph of the actual specimen which was cleared and stained for calcium salts. A quantitative method of evaluating the discriminating ability of each technique is described and used to compare these images. Xeroradiography and Kodak Crystallex film clearly excelled other imaging methods. The value of using a biological test object is discussed.", "PMID": 953386}
{"id": "pubmed23n0032_629", "title": "Irradiation to the thyroid gland at cardiac catheterization and angiocardiography in children.", "content": "Irradiation to the thyroid gland was measured during cardiac catheterization in 16 infants and 30 children aged 1 to 13 years. The doses from fluoroscopy, full-scale angiocardiography, and cineradiography were measured separately with thermoluminescent dosimeters and, in addition, the exposure-area product was assessed during fluoroscopy with a transparent ionization chamber. Median value of the absorbed dose to the thyroid from the complete heart investigation was 370 mrad in infants and 260 mrad in children. The highest values and also the greatest range in dosage were observed in infants. The quotient of the thyroid-absorbed dose divided by the integral dose or the dose per exposed film area was also highest in infants, due to anatomical conditions. The absorbed dose to the thyroid during heart catheterization comes almost exclusively from scattered radiation. By appropriate examination technique this dose can be kept within acceptable limits.", "contents": "Irradiation to the thyroid gland at cardiac catheterization and angiocardiography in children. Irradiation to the thyroid gland was measured during cardiac catheterization in 16 infants and 30 children aged 1 to 13 years. The doses from fluoroscopy, full-scale angiocardiography, and cineradiography were measured separately with thermoluminescent dosimeters and, in addition, the exposure-area product was assessed during fluoroscopy with a transparent ionization chamber. Median value of the absorbed dose to the thyroid from the complete heart investigation was 370 mrad in infants and 260 mrad in children. The highest values and also the greatest range in dosage were observed in infants. The quotient of the thyroid-absorbed dose divided by the integral dose or the dose per exposed film area was also highest in infants, due to anatomical conditions. The absorbed dose to the thyroid during heart catheterization comes almost exclusively from scattered radiation. By appropriate examination technique this dose can be kept within acceptable limits.", "PMID": 953387}
{"id": "pubmed23n0032_630", "title": "Tolerance of rodent tails to necrosis after \"daily\" fractionated X rays or D-T neutrons.", "content": "The rat tail has been used as a model system to study the necrosis of an organized tissue following fractionated doses of collimated D-T neutrons or 290 kV X rays. RBE values for tail tolerance - 10 per cent of tails necrosing after the early skin reactions - rise from about 1-7 (single doses) to about 3-1 (16 fractions in 22 days). Neutron tolerance doses are almost independent of fractionation from 2 to 16 fractions. The tissues at risk are shown to be rather hypoxic. Early skin reaction levels can be used to predict the fraction of tails that will necrose. Early peak reactions for a given fraction of necrotic tails were slightly higher for neutrons than for X rays, and this difference was consistent for all the dose fractionation schedules employed.", "contents": "Tolerance of rodent tails to necrosis after \"daily\" fractionated X rays or D-T neutrons. The rat tail has been used as a model system to study the necrosis of an organized tissue following fractionated doses of collimated D-T neutrons or 290 kV X rays. RBE values for tail tolerance - 10 per cent of tails necrosing after the early skin reactions - rise from about 1-7 (single doses) to about 3-1 (16 fractions in 22 days). Neutron tolerance doses are almost independent of fractionation from 2 to 16 fractions. The tissues at risk are shown to be rather hypoxic. Early skin reaction levels can be used to predict the fraction of tails that will necrose. Early peak reactions for a given fraction of necrotic tails were slightly higher for neutrons than for X rays, and this difference was consistent for all the dose fractionation schedules employed.", "PMID": 953388}
{"id": "pubmed23n0032_631", "title": "Effects of fractionated irradiation on mouse lung and a phenomenon of slow repair.", "content": "The LD/50 between 40 and 180 days after irradiation of the thorax has been taken as a measure of lung damage in mice. The method has been used to derive the relationship between total dose, number of fractions (N) and overall treatment time (T). For X rays the results fit an Ellis type of relationship, i.e. total dose alphaN0-25 T0-07. This only applies for more than 8 fractions, below which N0-39 becomes a good fit to the results. For neutrons, the best fit is total dose alphaT0-07. Various possible explanations for the T factor are discussed, but for lung damage we attribute it to a slow repair process which possibly may only be observed in slowly proliferating tissues. Slow repair is about 100 times less rapid than Elkind-type of repair of sublethal damage and whereas Elkind repair is greatly reduced for irradiation with fast neutrons, relative to X rays, slow repair is not.", "contents": "Effects of fractionated irradiation on mouse lung and a phenomenon of slow repair. The LD/50 between 40 and 180 days after irradiation of the thorax has been taken as a measure of lung damage in mice. The method has been used to derive the relationship between total dose, number of fractions (N) and overall treatment time (T). For X rays the results fit an Ellis type of relationship, i.e. total dose alphaN0-25 T0-07. This only applies for more than 8 fractions, below which N0-39 becomes a good fit to the results. For neutrons, the best fit is total dose alphaT0-07. Various possible explanations for the T factor are discussed, but for lung damage we attribute it to a slow repair process which possibly may only be observed in slowly proliferating tissues. Slow repair is about 100 times less rapid than Elkind-type of repair of sublethal damage and whereas Elkind repair is greatly reduced for irradiation with fast neutrons, relative to X rays, slow repair is not.", "PMID": 953389}
{"id": "pubmed23n0032_632", "title": "Modification of the radiocurability of a syngeneic murine squamous carcinoma by its site of growth, by electron-affinic drugs, and by ICRF 159.", "content": "A series of TCD50 experiments using a spontaneously arising, syngeneically transplated, murine squamous carcinoma is reported. Tumours implanted intradermally and irradiated while they remained superficial had a small but nonetheless cure-limiting hypoxic fraction of cells (TCD50 3,324-4,257 rad). The hypoxic fraction increased dramatically when these tumours invaded the subcutaneous tissues, or when tumours were implanted subcutaneously (TCD50 greater than 5,544 rad). Pre-treatment of mice with the electron-affinic drug Ro-07-0582 at a dose of 1 mg/g i.p. 20-30 minutes before irradiation effectively eliminated the hypoxic element from superficaial tumours (TCD50 1.862 rad). The same drug at a dose of 0-25 mg/g reduced the TCD50 for superficial tumours by a factor of 1-87 (3,986-2,134 rad), while another electron affinic drug, metronidazole, given at a dose of 0-25 mg/g reduced the TCD50 by a factor of 1-28 (4,257-3,337 rad). Treatment of mice with the drug ICRF 159, 30 mug/g daily from the time of tumour cell injection until the day of irradiation, reduced the TCD50 in unclamped tumours by less than 1-19. However, a commensurate reduction of TCD50 in clamped tumours suggested that improved tumour vascularization could not explain fully the drug's \"radiosensitizing\" action. The unique single radiation dose curability of most human skin cancers is discussed in the light of the experimental results, and the possible clinical implication of an increase in hypoxic fraction with tumour invasion is raised.", "contents": "Modification of the radiocurability of a syngeneic murine squamous carcinoma by its site of growth, by electron-affinic drugs, and by ICRF 159. A series of TCD50 experiments using a spontaneously arising, syngeneically transplated, murine squamous carcinoma is reported. Tumours implanted intradermally and irradiated while they remained superficial had a small but nonetheless cure-limiting hypoxic fraction of cells (TCD50 3,324-4,257 rad). The hypoxic fraction increased dramatically when these tumours invaded the subcutaneous tissues, or when tumours were implanted subcutaneously (TCD50 greater than 5,544 rad). Pre-treatment of mice with the electron-affinic drug Ro-07-0582 at a dose of 1 mg/g i.p. 20-30 minutes before irradiation effectively eliminated the hypoxic element from superficaial tumours (TCD50 1.862 rad). The same drug at a dose of 0-25 mg/g reduced the TCD50 for superficial tumours by a factor of 1-87 (3,986-2,134 rad), while another electron affinic drug, metronidazole, given at a dose of 0-25 mg/g reduced the TCD50 by a factor of 1-28 (4,257-3,337 rad). Treatment of mice with the drug ICRF 159, 30 mug/g daily from the time of tumour cell injection until the day of irradiation, reduced the TCD50 in unclamped tumours by less than 1-19. However, a commensurate reduction of TCD50 in clamped tumours suggested that improved tumour vascularization could not explain fully the drug's \"radiosensitizing\" action. The unique single radiation dose curability of most human skin cancers is discussed in the light of the experimental results, and the possible clinical implication of an increase in hypoxic fraction with tumour invasion is raised.", "PMID": 953390}
{"id": "pubmed23n0032_633", "title": "An after loading colpostat (using standard source tubes).", "content": "An afterloading system for the treatment of gynaecological patients is described. It uses a rigid tandem to which afterloading colpostats are attached. The catheters for afterloading the colpostats are flexible thus minimizing patient discomfort. A constant 90 deg. alignment of source tubes between the uterine tandem and colpostats is achieved.", "contents": "An after loading colpostat (using standard source tubes). An afterloading system for the treatment of gynaecological patients is described. It uses a rigid tandem to which afterloading colpostats are attached. The catheters for afterloading the colpostats are flexible thus minimizing patient discomfort. A constant 90 deg. alignment of source tubes between the uterine tandem and colpostats is achieved.", "PMID": 953391}
{"id": "pubmed23n0032_634", "title": "Multiple superficial gastric erosions due to Crohn's disease of the stomach. Radiologic and endoscopic diagnosis.", "content": "A case of Crohn's disease involving the stomach and duodenum is presented. The patient had a four-and-a-half year history of ankylosing spondylitis. Double contrast radiography of the stomach and duodenum showed multiple superficial gastric erosions and a duodenal ulcer. There were typical changes of Crohn's disease in the terminal ileum. At endoscopy, numerous superficial erosions were seen in the stomach and duodenum and biopsies of the stomach showed granulomatous inflammation, consistent with Crohn's disease. These lesions are similar to those seen in the colon in the early stages of granulomatous colitis and it is likely that this represents the early stage of Crohn's disease in the stomach. With increasing application of double-contrast radiography and endoscopy, these lesions may be found more frequently.", "contents": "Multiple superficial gastric erosions due to Crohn's disease of the stomach. Radiologic and endoscopic diagnosis. A case of Crohn's disease involving the stomach and duodenum is presented. The patient had a four-and-a-half year history of ankylosing spondylitis. Double contrast radiography of the stomach and duodenum showed multiple superficial gastric erosions and a duodenal ulcer. There were typical changes of Crohn's disease in the terminal ileum. At endoscopy, numerous superficial erosions were seen in the stomach and duodenum and biopsies of the stomach showed granulomatous inflammation, consistent with Crohn's disease. These lesions are similar to those seen in the colon in the early stages of granulomatous colitis and it is likely that this represents the early stage of Crohn's disease in the stomach. With increasing application of double-contrast radiography and endoscopy, these lesions may be found more frequently.", "PMID": 953393}
{"id": "pubmed23n0032_635", "title": "The surgical management of tuberculous lower ureteric stricture.", "content": "25 years' experience of the open surgical management of the lower ureteric stricture is reviewed. An extravesical anti-reflux tunnel procedure has been found to be simple and effective. There is still a place for the Boari procedure or psoas hitch, particularly in the management of a long stricture of when gross thickening of the ureter makes the formation of a tunnel of effective length impracticable.", "contents": "The surgical management of tuberculous lower ureteric stricture. 25 years' experience of the open surgical management of the lower ureteric stricture is reviewed. An extravesical anti-reflux tunnel procedure has been found to be simple and effective. There is still a place for the Boari procedure or psoas hitch, particularly in the management of a long stricture of when gross thickening of the ureter makes the formation of a tunnel of effective length impracticable.", "PMID": 953413}
{"id": "pubmed23n0032_636", "title": "Traumatic avulsion of the ureter with obstruction, pseudocyst formation and hypertension.", "content": "A further case of traumatic avulsion of the upper ureter is described; it was complicated by hypertension and treated by removal of the pseudocyst. Delay in diagnosis should not be a contraindication to reconstructive surgery which should always be attempted if the kidney is functioning. The possibility of renal artery ischaemia should be considered when hypertension develops in the presence of a pseduocyst.", "contents": "Traumatic avulsion of the ureter with obstruction, pseudocyst formation and hypertension. A further case of traumatic avulsion of the upper ureter is described; it was complicated by hypertension and treated by removal of the pseudocyst. Delay in diagnosis should not be a contraindication to reconstructive surgery which should always be attempted if the kidney is functioning. The possibility of renal artery ischaemia should be considered when hypertension develops in the presence of a pseduocyst.", "PMID": 953415}
{"id": "pubmed23n0032_637", "title": "Influence of vesico-ureteral reflux on the response to treatment of urinary tract infections in female children.", "content": "3 groups of female children, comprising a total of 169 patients, were followed through successive episodes of urinary tract infection with respect to recurrence of infection. Group A consisted of 66 patients with no evidence of vesico-ureteral reflux. Group B comprised 42 patients with demonstrated vesico-ureteral reflux. The infections in these patients were treated with medical therapy alone and the reflux was not corrected. Group C consisted of 61 children who had surgical correction of vesico-ureteral reflux. While Groups B and C were not strictly comparable, the data indicate that there was no demonstrable difference in the rate of urinary tract infection among the 3 groups. Following short-term specific antibacterial therapy of a new infection, about 20% of children in each group were \"cured\", i.e., had no further infections during the 12 months or more that they were subsequently followed by the authors. Patients with less severe grades of reflux treated by medication alone (Group B) experienced no greater rate of reinfection than those children presenting with infections in the absence of vesico-ureteral reflux. In those children whose infections could not be adequately controlled by antimicrobial therapy, and those patients with severe (Grade III) reflux, surgery was performed to eliminate the vesico-ureteral reflux. The data also reinforced the concept that correction of reflux does not eliminate the need for constant vigilance and proper treatment of recurrent infections even after successful surgery has been carried out.", "contents": "Influence of vesico-ureteral reflux on the response to treatment of urinary tract infections in female children. 3 groups of female children, comprising a total of 169 patients, were followed through successive episodes of urinary tract infection with respect to recurrence of infection. Group A consisted of 66 patients with no evidence of vesico-ureteral reflux. Group B comprised 42 patients with demonstrated vesico-ureteral reflux. The infections in these patients were treated with medical therapy alone and the reflux was not corrected. Group C consisted of 61 children who had surgical correction of vesico-ureteral reflux. While Groups B and C were not strictly comparable, the data indicate that there was no demonstrable difference in the rate of urinary tract infection among the 3 groups. Following short-term specific antibacterial therapy of a new infection, about 20% of children in each group were \"cured\", i.e., had no further infections during the 12 months or more that they were subsequently followed by the authors. Patients with less severe grades of reflux treated by medication alone (Group B) experienced no greater rate of reinfection than those children presenting with infections in the absence of vesico-ureteral reflux. In those children whose infections could not be adequately controlled by antimicrobial therapy, and those patients with severe (Grade III) reflux, surgery was performed to eliminate the vesico-ureteral reflux. The data also reinforced the concept that correction of reflux does not eliminate the need for constant vigilance and proper treatment of recurrent infections even after successful surgery has been carried out.", "PMID": 953416}
{"id": "pubmed23n0032_638", "title": "Percutaneous suprapubic cystostomy after spinal cord injury.", "content": "The results of treatment of urinary bladder paralysis with small calibre (9 Charriere) suprapubic drainage in 41 male patients are described. It was found that suprapubic drainage could be maintained for an average of 8 weeks and that the urine in 43% of patients remained sterile for an average of 7 weeks. This technique merits further trial, both in the paraplegic patient and in normal patients requiring catheterisation because of obstruction of the lower urinary tract.", "contents": "Percutaneous suprapubic cystostomy after spinal cord injury. The results of treatment of urinary bladder paralysis with small calibre (9 Charriere) suprapubic drainage in 41 male patients are described. It was found that suprapubic drainage could be maintained for an average of 8 weeks and that the urine in 43% of patients remained sterile for an average of 7 weeks. This technique merits further trial, both in the paraplegic patient and in normal patients requiring catheterisation because of obstruction of the lower urinary tract.", "PMID": 953418}
{"id": "pubmed23n0032_639", "title": "Asymptomatic phaeochromocytoma of the bladder co-existing with carcinoma.", "content": "A case of asymptomatic phaeochromocytoma occurring in co-existence with a transitional cell carcinoma of the urinary bladder is reported and the significant characteristics discussed.", "contents": "Asymptomatic phaeochromocytoma of the bladder co-existing with carcinoma. A case of asymptomatic phaeochromocytoma occurring in co-existence with a transitional cell carcinoma of the urinary bladder is reported and the significant characteristics discussed.", "PMID": 953419}
{"id": "pubmed23n0032_640", "title": "Male epispadias: surgical repairs of urethropenile deformity.", "content": "A surgical technique for the repair of complete epispadias, with emphasis on correction of the urethropenile deformity and attempt to restore normal anatomic relations has been presented. The epispadiac orifice is dissected and followed proximally to beyond the bladder neck, then brought out perineally between the bifurcated crura. The penis is denuded from its skin, then embedded in the scrotum, except for the glans penis which protrudes from the midscrotum. Penile skin is folded into a tube to be used later for urethral reconstruction. Excellent cosmetic and functional results are consistently obtained by this multi-stage technique.", "contents": "Male epispadias: surgical repairs of urethropenile deformity. A surgical technique for the repair of complete epispadias, with emphasis on correction of the urethropenile deformity and attempt to restore normal anatomic relations has been presented. The epispadiac orifice is dissected and followed proximally to beyond the bladder neck, then brought out perineally between the bifurcated crura. The penis is denuded from its skin, then embedded in the scrotum, except for the glans penis which protrudes from the midscrotum. Penile skin is folded into a tube to be used later for urethral reconstruction. Excellent cosmetic and functional results are consistently obtained by this multi-stage technique.", "PMID": 953420}
{"id": "pubmed23n0032_641", "title": "An unusual case of right and left testicular arteries arching over the left renal vein.", "content": "An unusual case in which both testicular arteries arched over the left renal vein is described. A developmental explanation for the unusual vascular pattern is given. The possible importance of this and similar anomalies in the causation of varicocoele and orthostatic proteinuria is discussed.", "contents": "An unusual case of right and left testicular arteries arching over the left renal vein. An unusual case in which both testicular arteries arched over the left renal vein is described. A developmental explanation for the unusual vascular pattern is given. The possible importance of this and similar anomalies in the causation of varicocoele and orthostatic proteinuria is discussed.", "PMID": 953421}
{"id": "pubmed23n0032_642", "title": "A.I.D.--a review of 200 cases.", "content": "2OO consecutive cases of couples referred for A.I.D. are reviewed. The reasons for referral are given and the technique and donors are discussed. It is estimated that about 85% of women who receive donor insemination will achieve pregnancy if they presevere. Infertility factors are common in women who present for donor insemination and this usually takes the form of ovarian follicular deficiency, probably with non-ovulation. It is suggested that a biphasic basal temperature record or a secretory endometrium in the luteal phase are unreliable tests for ovulation.", "contents": "A.I.D.--a review of 200 cases. 2OO consecutive cases of couples referred for A.I.D. are reviewed. The reasons for referral are given and the technique and donors are discussed. It is estimated that about 85% of women who receive donor insemination will achieve pregnancy if they presevere. Infertility factors are common in women who present for donor insemination and this usually takes the form of ovarian follicular deficiency, probably with non-ovulation. It is suggested that a biphasic basal temperature record or a secretory endometrium in the luteal phase are unreliable tests for ovulation.", "PMID": 953422}
{"id": "pubmed23n0032_643", "title": "Ascorbic acid levels in stored blood and in patients undergoing surgery after blood transfusion.", "content": "Blood was obtained from 11 healthy voluteers, mixed with two standard types of anticoagulant used in blood transfusion centres and stored for 21-28 days at 4 degrees C. Leucocyte ascorbic acid (LAA) fell to deficient levels after 7 days in all cases. There were no corresponding changes in plasma ascorbic acid (PAA) levels. LAA and PAA were measured before, during and after surgery in 5 control patients who underwent definitive operations for benign peptic ulceration and in 4 patients under-going surgery for bleeding peptic ulceration. The average amount of blood administered to the latter group was 10 units. There was a fall in LAA and PAA in both groups of patients after operation. This fall had returned to normal by 7 days in the controls, but the LAA remained at a deficient level at 7 days in the patients who had bled. Deficient ascorbic acid in stored blood may contribute to low leucocyte ascorbic acid levels in patients after blood transfusion and may contribute to the increased complication rate when surgery is undertaken in these patients.", "contents": "Ascorbic acid levels in stored blood and in patients undergoing surgery after blood transfusion. Blood was obtained from 11 healthy voluteers, mixed with two standard types of anticoagulant used in blood transfusion centres and stored for 21-28 days at 4 degrees C. Leucocyte ascorbic acid (LAA) fell to deficient levels after 7 days in all cases. There were no corresponding changes in plasma ascorbic acid (PAA) levels. LAA and PAA were measured before, during and after surgery in 5 control patients who underwent definitive operations for benign peptic ulceration and in 4 patients under-going surgery for bleeding peptic ulceration. The average amount of blood administered to the latter group was 10 units. There was a fall in LAA and PAA in both groups of patients after operation. This fall had returned to normal by 7 days in the controls, but the LAA remained at a deficient level at 7 days in the patients who had bled. Deficient ascorbic acid in stored blood may contribute to low leucocyte ascorbic acid levels in patients after blood transfusion and may contribute to the increased complication rate when surgery is undertaken in these patients.", "PMID": 953445}
{"id": "pubmed23n0032_644", "title": "Bronchopleural fistula: a review of 86 cases.", "content": "A review of all the bronchopleural fistulas which developed following pulmonary resection in the Department of Cardio-Vascular and Thoracic Surgery of The London Hospital between 1953 and 1973 is presented. During this period 3150 pulmonary resections were performed for various conditions and 86 fistulas occurred, an incidence of 2-7 per cent. The aetiology, diagnosis and treatment of these fistulas are discussed.", "contents": "Bronchopleural fistula: a review of 86 cases. A review of all the bronchopleural fistulas which developed following pulmonary resection in the Department of Cardio-Vascular and Thoracic Surgery of The London Hospital between 1953 and 1973 is presented. During this period 3150 pulmonary resections were performed for various conditions and 86 fistulas occurred, an incidence of 2-7 per cent. The aetiology, diagnosis and treatment of these fistulas are discussed.", "PMID": 953446}
{"id": "pubmed23n0032_645", "title": "Postgastrectomy afferent loop obstruction due to efferent loop herniation simulating acute pancreatitis.", "content": "A case of afferent loop obstruction due to efferent loop retro-anastomatic herniation following a Polya gastrectomy is presented. The clinical picture closely mimicked that of acute pancreatitis. The difficulty in diagnosis in this situation is discussed and the importance of operative techniques in its prevention emphasized.", "contents": "Postgastrectomy afferent loop obstruction due to efferent loop herniation simulating acute pancreatitis. A case of afferent loop obstruction due to efferent loop retro-anastomatic herniation following a Polya gastrectomy is presented. The clinical picture closely mimicked that of acute pancreatitis. The difficulty in diagnosis in this situation is discussed and the importance of operative techniques in its prevention emphasized.", "PMID": 953447}
{"id": "pubmed23n0032_646", "title": "Multivariate analysis of clinical and operative findings associated with bilary sepsis.", "content": "A cluster analysis of 17 preoperative and 10 operative variables has been undertaken from a prospective study of 181 patients undergoing operations on the biliary tract in order to identify those with organisms in the bile who would therefore benefit from prophylactic antibiotic cover. The analysis defined populations of high risk (45 patients) and low risk (136 patients) in which the incidence of infected bile was 67 and 19 per cent respectively (P less than 0-001). Eight variables were more common in the high risk than in the low risk group (P less than 0-001). These \"high risk factors\" were: jaundice at operation, recent rigoors, emergency operation or one within 4 weeks of an emergency admission, age over 70 years, previous biliary operation, common duct obstruction and stones in the bile duct. In 67 patients where one or more \"high risk factors\" was present, the bile contained organisms in 37 (53 percent); when 4 \"high risk factors\" occurred in the same patient (16 cases) biliary organisms were isolated in 14(87 per cent). We conclude that the presence of one or more \"high risk factors\" indicates a patient in whom prophylactic antibiotics would be advised during an operation on the biliary tract.", "contents": "Multivariate analysis of clinical and operative findings associated with bilary sepsis. A cluster analysis of 17 preoperative and 10 operative variables has been undertaken from a prospective study of 181 patients undergoing operations on the biliary tract in order to identify those with organisms in the bile who would therefore benefit from prophylactic antibiotic cover. The analysis defined populations of high risk (45 patients) and low risk (136 patients) in which the incidence of infected bile was 67 and 19 per cent respectively (P less than 0-001). Eight variables were more common in the high risk than in the low risk group (P less than 0-001). These \"high risk factors\" were: jaundice at operation, recent rigoors, emergency operation or one within 4 weeks of an emergency admission, age over 70 years, previous biliary operation, common duct obstruction and stones in the bile duct. In 67 patients where one or more \"high risk factors\" was present, the bile contained organisms in 37 (53 percent); when 4 \"high risk factors\" occurred in the same patient (16 cases) biliary organisms were isolated in 14(87 per cent). We conclude that the presence of one or more \"high risk factors\" indicates a patient in whom prophylactic antibiotics would be advised during an operation on the biliary tract.", "PMID": 953448}
{"id": "pubmed23n0032_647", "title": "Colonic lymphoma complicating ulcerative colitis.", "content": "Colonic lymphoma is a rare complication of ulcerative colitis. Two cases are described in patients who had had ulcerative colitis for 12 and 22 years respectively. Both patients presented with a recent change in their symptoms, which had become increasingly severe and which had not remitted with customary treatment for ulcerative colitis. Physical and haematological examinations revealed no evidence of generalized lymphoma, though barium enema studies indicated the sites of the lymphomatous lesions superimposed on chronic ulcerative colitis which were confirmed at operation and biopsy.", "contents": "Colonic lymphoma complicating ulcerative colitis. Colonic lymphoma is a rare complication of ulcerative colitis. Two cases are described in patients who had had ulcerative colitis for 12 and 22 years respectively. Both patients presented with a recent change in their symptoms, which had become increasingly severe and which had not remitted with customary treatment for ulcerative colitis. Physical and haematological examinations revealed no evidence of generalized lymphoma, though barium enema studies indicated the sites of the lymphomatous lesions superimposed on chronic ulcerative colitis which were confirmed at operation and biopsy.", "PMID": 953449}
{"id": "pubmed23n0032_648", "title": "Anorectal function after major resections of the sacrum with bilateral or unilateral sacrifice of sacral nerves.", "content": "The anorectal function in 3 patients with bilateral and 4 patients with unilateral well-defined loss of sacral nerves after radical tumour excision was studied by clinical examination and by simultaneous registration of the following variables: volume and pressure in the rectum, pressure in the internal anal sphincter area and myoelectrical activity in the external anal sphincter. The patients with bilateral loss of sacral nerves had serious impairment of function. Constipation was their only safeguard against incontinence. The preservation of the first and second sacral nerves bilaterally was not sufficient for discrimination between different qualities of rectal contents passing the anal canal. The sensation of rectal distension was also impaired. The reflex pattern of the internal anal sphincter was, however, intact. The external anal sphincter displayed a weak spontaneous myoelectrical activity in the patients who had at least one second sacral nerve intact, and a weak increase of the activity could be induced voluntarily. The normal transient increase of myoelectrical discharge from the external anal sphincter in response to rectal distension could not, however, be elicited. In patients with total unilateral loss of the sacral nerves no significant impairment of anorectal function was noted. Total one-sided denervation implied deficient sensibility of the anal canal unilaterally, but no disturbance of sphincter function as judged from the reflex response of the internal and external anal sphincters to rectal distension.", "contents": "Anorectal function after major resections of the sacrum with bilateral or unilateral sacrifice of sacral nerves. The anorectal function in 3 patients with bilateral and 4 patients with unilateral well-defined loss of sacral nerves after radical tumour excision was studied by clinical examination and by simultaneous registration of the following variables: volume and pressure in the rectum, pressure in the internal anal sphincter area and myoelectrical activity in the external anal sphincter. The patients with bilateral loss of sacral nerves had serious impairment of function. Constipation was their only safeguard against incontinence. The preservation of the first and second sacral nerves bilaterally was not sufficient for discrimination between different qualities of rectal contents passing the anal canal. The sensation of rectal distension was also impaired. The reflex pattern of the internal anal sphincter was, however, intact. The external anal sphincter displayed a weak spontaneous myoelectrical activity in the patients who had at least one second sacral nerve intact, and a weak increase of the activity could be induced voluntarily. The normal transient increase of myoelectrical discharge from the external anal sphincter in response to rectal distension could not, however, be elicited. In patients with total unilateral loss of the sacral nerves no significant impairment of anorectal function was noted. Total one-sided denervation implied deficient sensibility of the anal canal unilaterally, but no disturbance of sphincter function as judged from the reflex response of the internal and external anal sphincters to rectal distension.", "PMID": 953450}
{"id": "pubmed23n0032_649", "title": "A study of perineal wound healing after abdominoperineal resection.", "content": "A comparative study of 53 cases has revealed that a technique of complete primary closure of the perineal wound after abdominoperineal resection of the rectum and anal canal appears to be a superior, more rational approach than other orthodox techniques. (It is unsuitable for any case contaminated with pus or faeces during operation.) Redivac apparatus used through a separate route for continuous drainage from the sacral cavity has made the postoperative care easier for nurses and surgeons and this period more comfortable for the patient. It provides a simple method compared with other suction apparatus used for the same purpose. Of the 53 cases, 12 were operated on using a traditional technique involving the closure of the perineal wound around a tube drain connected to an underwater seal, while in the remaining 41 the approach described here was used. Primary healing of the perineal wound with the later approach was obtained in about 88 per cent. With the other technique the figures were 34 and 66 per cent respectively for early healing within 3 weeks and delayed healing between 3-8 weeks. Primary healing of the perineal wound reduces the total stay in hospital and the morbidity.", "contents": "A study of perineal wound healing after abdominoperineal resection. A comparative study of 53 cases has revealed that a technique of complete primary closure of the perineal wound after abdominoperineal resection of the rectum and anal canal appears to be a superior, more rational approach than other orthodox techniques. (It is unsuitable for any case contaminated with pus or faeces during operation.) Redivac apparatus used through a separate route for continuous drainage from the sacral cavity has made the postoperative care easier for nurses and surgeons and this period more comfortable for the patient. It provides a simple method compared with other suction apparatus used for the same purpose. Of the 53 cases, 12 were operated on using a traditional technique involving the closure of the perineal wound around a tube drain connected to an underwater seal, while in the remaining 41 the approach described here was used. Primary healing of the perineal wound with the later approach was obtained in about 88 per cent. With the other technique the figures were 34 and 66 per cent respectively for early healing within 3 weeks and delayed healing between 3-8 weeks. Primary healing of the perineal wound reduces the total stay in hospital and the morbidity.", "PMID": 953451}
{"id": "pubmed23n0032_650", "title": "Altered viscosity and yield stress in patients with abdominal malignancy: relationship to deep vein thrombosis.", "content": "Twenty-eight patients undergoing laparotomy were studied. Blood viscosity at both high and low rates of shear and a yield stress index were measured preoperatively and correlated with the incidence of post-operative deep vein thrombosis (DVT). There was a correlation between a raised index of yield stress and the incidence of DVT. Patients with cancer had a very high incidence of postoperative DVT and a high index of yield stress.", "contents": "Altered viscosity and yield stress in patients with abdominal malignancy: relationship to deep vein thrombosis. Twenty-eight patients undergoing laparotomy were studied. Blood viscosity at both high and low rates of shear and a yield stress index were measured preoperatively and correlated with the incidence of post-operative deep vein thrombosis (DVT). There was a correlation between a raised index of yield stress and the incidence of DVT. Patients with cancer had a very high incidence of postoperative DVT and a high index of yield stress.", "PMID": 953452}
{"id": "pubmed23n0032_651", "title": "\"Hitch-hike\" grafts for limb salvage in peripheral arterial disease.", "content": "A technique of arterial reconstruction suitable for patients with extensive arterial occlusions and for previous failed arterial surgery is described. The \"hitch-hike\" graft consists of a proximal limb of 6-mm velour Dacron prosthesis and a distal limb of autogenous vein. The intermediate prosthesis-to-vein anastomosis is made into an endarterectomized segment of upper popliteal artery. The results in the first 16 limbs are described. Eleven grafts are functioning from 2 to 14 months after operation and 5 of these have functioned for more than 1 year. The advantages of the technique are: long arterial occlusions may be bridged; autogenous vein is used to cross the knee joint; good measured blood flows may be demonstrated at operation; acceptable patency rates may be obtained up to 1 year.", "contents": "\"Hitch-hike\" grafts for limb salvage in peripheral arterial disease. A technique of arterial reconstruction suitable for patients with extensive arterial occlusions and for previous failed arterial surgery is described. The \"hitch-hike\" graft consists of a proximal limb of 6-mm velour Dacron prosthesis and a distal limb of autogenous vein. The intermediate prosthesis-to-vein anastomosis is made into an endarterectomized segment of upper popliteal artery. The results in the first 16 limbs are described. Eleven grafts are functioning from 2 to 14 months after operation and 5 of these have functioned for more than 1 year. The advantages of the technique are: long arterial occlusions may be bridged; autogenous vein is used to cross the knee joint; good measured blood flows may be demonstrated at operation; acceptable patency rates may be obtained up to 1 year.", "PMID": 953453}
{"id": "pubmed23n0032_652", "title": "Simple mastectomy and pectoral node biopsy.", "content": "Evidence is presented to support the view that biopsy of the pectoral and axillary tail nodes obtained at simple (total) mastectomy gives a reliable estimate of axillary node status. The technique is described.", "contents": "Simple mastectomy and pectoral node biopsy. Evidence is presented to support the view that biopsy of the pectoral and axillary tail nodes obtained at simple (total) mastectomy gives a reliable estimate of axillary node status. The technique is described.", "PMID": 953454}
{"id": "pubmed23n0032_653", "title": "The relationship between thermographic findings and size and depth of tumour in carcinoma of the breast.", "content": "Fifty thermograms of histologically proved breast carcinomas have been analysed without knowledge of the clinical or mammographic findings. Thermographic asymmetry between the breasts was found in 76 percent of the patients, but in only 64 per cent of the patients was the correct side indicated. Poor correlations has also been found between the thermographic findings and the size of a tumour and its distance from the skin.", "contents": "The relationship between thermographic findings and size and depth of tumour in carcinoma of the breast. Fifty thermograms of histologically proved breast carcinomas have been analysed without knowledge of the clinical or mammographic findings. Thermographic asymmetry between the breasts was found in 76 percent of the patients, but in only 64 per cent of the patients was the correct side indicated. Poor correlations has also been found between the thermographic findings and the size of a tumour and its distance from the skin.", "PMID": 953455}
{"id": "pubmed23n0032_654", "title": "Mortality of oesophagal surgery in the elderly.", "content": "The operative mortality in pulmonary surgery was found to be four times higher in patients aged above 70 compared with those below 65. A similar study has been carried out in patients undergoing oesophageal surgery. A corresponding increase in operative mortality with advancing age was not found in this group. Anastomotic leakage remains the most common cause of operative mortality, the incidence of which is no higher in the elderly, though they are more liable to general complications. The overall mortality figures are comparable. The surgical approach to carcinoma of the oesophagus must be radical, and advanced age should not be a reason for withholding surgery.", "contents": "Mortality of oesophagal surgery in the elderly. The operative mortality in pulmonary surgery was found to be four times higher in patients aged above 70 compared with those below 65. A similar study has been carried out in patients undergoing oesophageal surgery. A corresponding increase in operative mortality with advancing age was not found in this group. Anastomotic leakage remains the most common cause of operative mortality, the incidence of which is no higher in the elderly, though they are more liable to general complications. The overall mortality figures are comparable. The surgical approach to carcinoma of the oesophagus must be radical, and advanced age should not be a reason for withholding surgery.", "PMID": 953456}
{"id": "pubmed23n0032_655", "title": "The effect of posture on the emptying of the intrathoracic vagotomized stomach.", "content": "Ten patients were studied at periods ranging from 6 months to 25 years after oesophagogastrostomy and gastric drainage. Gastric emptying studies and overnight gastric aspirates in both the supine and erect positions were carried out. Evidence was found in these patients of increased duodenogastric reflux and poor gastric emptying in the supine posture. All of the patients had gastritis, and some had gastric mucosal ulceration. These findings have led to the conclusion that in the supine posture the combination of duodenogastric reflux and poor gastric emptying leads to gastric mucosal damage.", "contents": "The effect of posture on the emptying of the intrathoracic vagotomized stomach. Ten patients were studied at periods ranging from 6 months to 25 years after oesophagogastrostomy and gastric drainage. Gastric emptying studies and overnight gastric aspirates in both the supine and erect positions were carried out. Evidence was found in these patients of increased duodenogastric reflux and poor gastric emptying in the supine posture. All of the patients had gastritis, and some had gastric mucosal ulceration. These findings have led to the conclusion that in the supine posture the combination of duodenogastric reflux and poor gastric emptying leads to gastric mucosal damage.", "PMID": 953457}
{"id": "pubmed23n0032_656", "title": "The distribution and early morphology of the chronic gastric mucosal reaction.", "content": "The distribution of the chronic gastric mucosal reaction (CGMR) that resulted from the exposure of the mucosa to refluxing duodenal contents did not suggest that one area of the stomach was more susceptible than another. The earliest histological appearances of established CGMR as seen 3 months after surgery were epithelial proliferation and pseudy-pyloric metaplasia.", "contents": "The distribution and early morphology of the chronic gastric mucosal reaction. The distribution of the chronic gastric mucosal reaction (CGMR) that resulted from the exposure of the mucosa to refluxing duodenal contents did not suggest that one area of the stomach was more susceptible than another. The earliest histological appearances of established CGMR as seen 3 months after surgery were epithelial proliferation and pseudy-pyloric metaplasia.", "PMID": 953458}
{"id": "pubmed23n0032_657", "title": "Is the lesser curvature an area especially susceptible to injury by duodenal contents?", "content": "The lesser curvature does not appear to be an area of increased susceptibility to injury. The fact that this is the usual area for the development of chronic gastric ulceration could in part be explained by reflux of duodenal contents along the lesser curvature.", "contents": "Is the lesser curvature an area especially susceptible to injury by duodenal contents? The lesser curvature does not appear to be an area of increased susceptibility to injury. The fact that this is the usual area for the development of chronic gastric ulceration could in part be explained by reflux of duodenal contents along the lesser curvature.", "PMID": 953459}
{"id": "pubmed23n0032_658", "title": "The role of the pyloric antrum and Hofmeister value in the development of the chronic gastric mucosal reaction.", "content": "A limited chronic gastric mucosal reaction (CGMR) develops when duodenal contents enter the stomach with an intact pyloric antrum. However, after antrectomy the same stimulus leads to a widespread CGMR. The pyloric antrum, therefore, seems to influence the development of the CGMR. A Hofmeister valve included in a Polya gastrectomy does not prevent the development of the VGMR, presumably because reflux is not prevented.", "contents": "The role of the pyloric antrum and Hofmeister value in the development of the chronic gastric mucosal reaction. A limited chronic gastric mucosal reaction (CGMR) develops when duodenal contents enter the stomach with an intact pyloric antrum. However, after antrectomy the same stimulus leads to a widespread CGMR. The pyloric antrum, therefore, seems to influence the development of the CGMR. A Hofmeister valve included in a Polya gastrectomy does not prevent the development of the VGMR, presumably because reflux is not prevented.", "PMID": 953460}
{"id": "pubmed23n0032_659", "title": "Extragastric gastrinoma or G-cell hyperplasia of the antrum? The preoperative diagnosis in a case of hypergastrinaemia.", "content": "A patient is described with recurrent peptic ulceration, evidence of hypersecretion of gastric acid and excess circulating gastrin. The preoperative differentiation between extragastric \"gastrinoma\" and G-cell hyperplasia of the pyloric antrum as a source for the hypergastrinaemia can be made by immunofluorescent staining of an antral biopsy for \"gastrin cells\", and by measuring the response of the gastric antrum to an amino acid meal or secretion infusion stimulus. The site of excessive gastrin production determines the nature of the surgery required. A classification of recurrent peptic ulceration types is suggested.", "contents": "Extragastric gastrinoma or G-cell hyperplasia of the antrum? The preoperative diagnosis in a case of hypergastrinaemia. A patient is described with recurrent peptic ulceration, evidence of hypersecretion of gastric acid and excess circulating gastrin. The preoperative differentiation between extragastric \"gastrinoma\" and G-cell hyperplasia of the pyloric antrum as a source for the hypergastrinaemia can be made by immunofluorescent staining of an antral biopsy for \"gastrin cells\", and by measuring the response of the gastric antrum to an amino acid meal or secretion infusion stimulus. The site of excessive gastrin production determines the nature of the surgery required. A classification of recurrent peptic ulceration types is suggested.", "PMID": 953461}
{"id": "pubmed23n0032_660", "title": "The effect of metramide on the prolonged serum gastrin response to feeding observed in patients with duodenal ulcer.", "content": "The response of serum gastrin to a meal has been studied in 11 normal subjects and 16 patients with duodenal ulceration. The mean serum gastrin concentration rises after a meal to similar peak values in both normal subjects and duodenal ulcer patients, and fall to basal values with 3 hours in normal subjects. In duodenal ulcer patients the peak concentration is sustained throughout the 3 hour test period, and this response is not affected by the administration of Metiamide. It is concluded that the control of gastrin release is defective in duodenal ulceration, and this may be due to a failure of the antral pH feedback mechanism. Gastrin may be a primary pathogenetic factor in duodenal ulceration.", "contents": "The effect of metramide on the prolonged serum gastrin response to feeding observed in patients with duodenal ulcer. The response of serum gastrin to a meal has been studied in 11 normal subjects and 16 patients with duodenal ulceration. The mean serum gastrin concentration rises after a meal to similar peak values in both normal subjects and duodenal ulcer patients, and fall to basal values with 3 hours in normal subjects. In duodenal ulcer patients the peak concentration is sustained throughout the 3 hour test period, and this response is not affected by the administration of Metiamide. It is concluded that the control of gastrin release is defective in duodenal ulceration, and this may be due to a failure of the antral pH feedback mechanism. Gastrin may be a primary pathogenetic factor in duodenal ulceration.", "PMID": 953462}
{"id": "pubmed23n0032_661", "title": "Cancer of the stomach: a review of 854 patients.", "content": "A review is presented of 854 patients suffering from cancer of the stomach who attended St Bartholomew's Hospital between 1948 and 1962. The presentations and methods of investigations showed little change over the 15-year period, which is similar to other large series. The symptoms of anaemia and indigestion appear to be paramount in making an early diagnosis, and negative barium meal studies should not be accepted when these two symptoms are present. There are indications that early laporotomy and a more aggressive surgical approach may increase the 5-year survival rate.", "contents": "Cancer of the stomach: a review of 854 patients. A review is presented of 854 patients suffering from cancer of the stomach who attended St Bartholomew's Hospital between 1948 and 1962. The presentations and methods of investigations showed little change over the 15-year period, which is similar to other large series. The symptoms of anaemia and indigestion appear to be paramount in making an early diagnosis, and negative barium meal studies should not be accepted when these two symptoms are present. There are indications that early laporotomy and a more aggressive surgical approach may increase the 5-year survival rate.", "PMID": 953463}
{"id": "pubmed23n0032_662", "title": "Drug therapy for postragotomy gastric stasis.", "content": "An unusual but well-recognized complication of truncal vagotomy with a drainage procedure is severe gastric stasis (atony). In 6 patients presenting with this problem drug therapy was initiated after mechanical obstruction of the gastric outlet was excluded by radiology. Bethanechol chloride was successful in stimulating gastric peristalsis in all the patients, with consequent improvement of symptoms in 5 out of the 6 subjects. Metoclopramide alone was of no value in starting peristalsis but of pssible benefit once gastric motility had begun.", "contents": "Drug therapy for postragotomy gastric stasis. An unusual but well-recognized complication of truncal vagotomy with a drainage procedure is severe gastric stasis (atony). In 6 patients presenting with this problem drug therapy was initiated after mechanical obstruction of the gastric outlet was excluded by radiology. Bethanechol chloride was successful in stimulating gastric peristalsis in all the patients, with consequent improvement of symptoms in 5 out of the 6 subjects. Metoclopramide alone was of no value in starting peristalsis but of pssible benefit once gastric motility had begun.", "PMID": 953464}
{"id": "pubmed23n0032_663", "title": "The relationship of plasma glucagon to the hyperglycaemia and hyperinsulinaemia of surgical operation.", "content": "In a controlled metabolic study of 42 patients undergoing abdominal surgery a significant increase in basal values of immunoreactive glucagon (IRG) was found 24 hours postoperatively. No correlation between the onset and duration of hyperglycaemia and the increase of IRG was observed. No increase in immunoreactive insulin (IRI) was seen during operation, but there was a rise 24 hours postoperatively. The infusion of arginine was accompanied by a rise in IRG both pre-and postoperatively, but despite the high values obtained there was no accompanying further increase in basal plasma glucose in the postoperative situation. The pre- and postoperative IRI responses were similar. Our evidence suggests that glucagon is not a primary mediator of the stress response, or of the hyperglycaemia of surgical operation in these patients.", "contents": "The relationship of plasma glucagon to the hyperglycaemia and hyperinsulinaemia of surgical operation. In a controlled metabolic study of 42 patients undergoing abdominal surgery a significant increase in basal values of immunoreactive glucagon (IRG) was found 24 hours postoperatively. No correlation between the onset and duration of hyperglycaemia and the increase of IRG was observed. No increase in immunoreactive insulin (IRI) was seen during operation, but there was a rise 24 hours postoperatively. The infusion of arginine was accompanied by a rise in IRG both pre-and postoperatively, but despite the high values obtained there was no accompanying further increase in basal plasma glucose in the postoperative situation. The pre- and postoperative IRI responses were similar. Our evidence suggests that glucagon is not a primary mediator of the stress response, or of the hyperglycaemia of surgical operation in these patients.", "PMID": 953465}
{"id": "pubmed23n0032_664", "title": "A conrolled trial of glucagon in experimental pancreatitis.", "content": "This study was designed to assess the value of glucagon infusion in pigs with experimental pancreatitis. The condition was induced by injection of bile into the pancreatic duct directly after intravenous injection of secretion. Macroscopic haemorrpagic pancreatitis ensued immediately, and was accompanied by a rise in the serum amylase of five to ten times. An experimental group of 15 pigs was given glucagon by continous infusion for 18 hours beginning 18 hours after induction of pancreatitis. A control group was given intravenous saline. Fourteen of the 15 treated animals survived for 1 week and 3 died within 2 weeks, after which the remaining animals were sacrificed. Ten of the 15 controls died within the first week. Serum amylase levels in both groups began to decline when infusion was commenced, but levels in the treated group were significantly lower than in the controls. Autopsy showed prominent peripancreatic granulation tissue in the treated animals, a significant incidence of pericarditis in both groups and pancreatic pseudocysts in 3 controls and 1 treated animal. These results suggest that glucagon effectively reduced the mortality in pigs with experimental pancreatitis and that a controlled clinical trial of treatment is justified.", "contents": "A conrolled trial of glucagon in experimental pancreatitis. This study was designed to assess the value of glucagon infusion in pigs with experimental pancreatitis. The condition was induced by injection of bile into the pancreatic duct directly after intravenous injection of secretion. Macroscopic haemorrpagic pancreatitis ensued immediately, and was accompanied by a rise in the serum amylase of five to ten times. An experimental group of 15 pigs was given glucagon by continous infusion for 18 hours beginning 18 hours after induction of pancreatitis. A control group was given intravenous saline. Fourteen of the 15 treated animals survived for 1 week and 3 died within 2 weeks, after which the remaining animals were sacrificed. Ten of the 15 controls died within the first week. Serum amylase levels in both groups began to decline when infusion was commenced, but levels in the treated group were significantly lower than in the controls. Autopsy showed prominent peripancreatic granulation tissue in the treated animals, a significant incidence of pericarditis in both groups and pancreatic pseudocysts in 3 controls and 1 treated animal. These results suggest that glucagon effectively reduced the mortality in pigs with experimental pancreatitis and that a controlled clinical trial of treatment is justified.", "PMID": 953466}
{"id": "pubmed23n0032_665", "title": "Ischaemic necrosis of the gallbladder following hepatic artery embolism.", "content": "A case of ischaemic necrosis of the gallbladder due to hepatic artery embolism diagnosed at operation and treated successfully by cholecystectomy and embolectomy is presented. The clinical and biochemical features are described, together with a short review of the literature.", "contents": "Ischaemic necrosis of the gallbladder following hepatic artery embolism. A case of ischaemic necrosis of the gallbladder due to hepatic artery embolism diagnosed at operation and treated successfully by cholecystectomy and embolectomy is presented. The clinical and biochemical features are described, together with a short review of the literature.", "PMID": 953467}
{"id": "pubmed23n0032_666", "title": "Peicystogastrostomy: internal drainage in the treatment of certain hydatid cysts of the liver.", "content": "The effectiveness and simplicity of drainage of hydatid cysts of the liver into the digestive tract are demonstrated. A new technique, pericystogastrostomy, is described.", "contents": "Peicystogastrostomy: internal drainage in the treatment of certain hydatid cysts of the liver. The effectiveness and simplicity of drainage of hydatid cysts of the liver into the digestive tract are demonstrated. A new technique, pericystogastrostomy, is described.", "PMID": 953468}
{"id": "pubmed23n0032_667", "title": "Cholecystostomy.", "content": "The role of cholecystostomy in the sugical treatment of gallstones has been considered. In a consecutive series of 558 patients who underwent surgery for gallstones, 30 (5-4 per cent) had cholecystostomy alone and a further 17 (3-1 per cent) cholecystostomy combined with exploration of the bile ducts. Cholecystostomy was done because cholecystectomy was technically very hazardous in 15 patients. In the remaining patients cholecystostomy was preferred to cholecystectomy because o f the poor general condition of the patient or the presence of pancreatitis. Of the 47 patients submitted to cholecystostomy, 2(4-2 per cent) died postoeratively and 4(8-4 percent) subsequently underwent cholecystectomy. Of 24 patients who were available for long term follow-up, only 1 patient had pain which was definitely considered to be due to gallstones, although 3 other patients also had abdominal pain, while 7 patients showed radiological evidience of gallstones. It is contended that cholecystostomy has a small but definite part to play in the immediate surgical treatment of gallstones and that the long term results of the operation are sufficiently good to justify its use in selected patients.", "contents": "Cholecystostomy. The role of cholecystostomy in the sugical treatment of gallstones has been considered. In a consecutive series of 558 patients who underwent surgery for gallstones, 30 (5-4 per cent) had cholecystostomy alone and a further 17 (3-1 per cent) cholecystostomy combined with exploration of the bile ducts. Cholecystostomy was done because cholecystectomy was technically very hazardous in 15 patients. In the remaining patients cholecystostomy was preferred to cholecystectomy because o f the poor general condition of the patient or the presence of pancreatitis. Of the 47 patients submitted to cholecystostomy, 2(4-2 per cent) died postoeratively and 4(8-4 percent) subsequently underwent cholecystectomy. Of 24 patients who were available for long term follow-up, only 1 patient had pain which was definitely considered to be due to gallstones, although 3 other patients also had abdominal pain, while 7 patients showed radiological evidience of gallstones. It is contended that cholecystostomy has a small but definite part to play in the immediate surgical treatment of gallstones and that the long term results of the operation are sufficiently good to justify its use in selected patients.", "PMID": 953469}
{"id": "pubmed23n0032_668", "title": "Intraperitoneal rupture ofhydronephrotic kidney.", "content": "Two cases of intraperitoneal rupture of hydronephrotic kidney are presented. The possibility of making a preoperative diagnosis and the type of management are discussed.", "contents": "Intraperitoneal rupture ofhydronephrotic kidney. Two cases of intraperitoneal rupture of hydronephrotic kidney are presented. The possibility of making a preoperative diagnosis and the type of management are discussed.", "PMID": 953470}
{"id": "pubmed23n0032_669", "title": "Follow-up study of left suprarenal to inferior mesenteric venous shunt for advanced breast cancer.", "content": "Major endocrine ablation therapy was carried out in 133 patients with advanced breast cancer. Of 40 patients undergoing left suprarenal to inferior mesenteric venous shunt with bilateral oophorectomy and right adrenalectomy, 16 cases (40 per cent) had an objective response. The mean postoperative survival of the patients with a positive response, those showing some response and those who failed to respond was 35-8, 26-5 and 8-8 months respectively. Thus, the results of the present procedure are comparable to those of adrenalectomy. No replacement therapy with corticoids was necessary. An increase in the response rate and extension of the survival time can be expected with the addition of postoperative long term maintenance therapy with chemotherapeutic agents and androgens.", "contents": "Follow-up study of left suprarenal to inferior mesenteric venous shunt for advanced breast cancer. Major endocrine ablation therapy was carried out in 133 patients with advanced breast cancer. Of 40 patients undergoing left suprarenal to inferior mesenteric venous shunt with bilateral oophorectomy and right adrenalectomy, 16 cases (40 per cent) had an objective response. The mean postoperative survival of the patients with a positive response, those showing some response and those who failed to respond was 35-8, 26-5 and 8-8 months respectively. Thus, the results of the present procedure are comparable to those of adrenalectomy. No replacement therapy with corticoids was necessary. An increase in the response rate and extension of the survival time can be expected with the addition of postoperative long term maintenance therapy with chemotherapeutic agents and androgens.", "PMID": 953471}
{"id": "pubmed23n0032_670", "title": "The haematological hazards of autotransfusion.", "content": "Fourteen heparinized dogs were autotransfused from 1-5 to 6 times calculated blood volume. Five animals retransfused 12 litres from an intraperitoneal bleed had marked decreases in all cellular elements, haematuria and a large (82 percent) drop in fibrinogen. All these animals died within 12 hours and post-mortem examination revealed evidence of disseminated intravascular coagulopathy. The dogs in which a smaller volume (3-6 litres) was recycled showed similar, but less devastating, changes. Haematocrit and platelet count dropped by a half and fibrinogen by 20 per cent. A leucocytosis of about 30 000/mm3 occurred within 24 hours. Autotransfusion of salvaged blood which was not allowed extravascular tissue contact significantly lessened these adverse effects. We conclude that the risk of cellular destruction and defibrination in large volume intra-operative autotransfusion is significant and must be weighed against its potential benefits in each case, and that red cell and platelet damage results primarily from extravascular tissue contact and is therefore unavoidable.", "contents": "The haematological hazards of autotransfusion. Fourteen heparinized dogs were autotransfused from 1-5 to 6 times calculated blood volume. Five animals retransfused 12 litres from an intraperitoneal bleed had marked decreases in all cellular elements, haematuria and a large (82 percent) drop in fibrinogen. All these animals died within 12 hours and post-mortem examination revealed evidence of disseminated intravascular coagulopathy. The dogs in which a smaller volume (3-6 litres) was recycled showed similar, but less devastating, changes. Haematocrit and platelet count dropped by a half and fibrinogen by 20 per cent. A leucocytosis of about 30 000/mm3 occurred within 24 hours. Autotransfusion of salvaged blood which was not allowed extravascular tissue contact significantly lessened these adverse effects. We conclude that the risk of cellular destruction and defibrination in large volume intra-operative autotransfusion is significant and must be weighed against its potential benefits in each case, and that red cell and platelet damage results primarily from extravascular tissue contact and is therefore unavoidable.", "PMID": 953472}
{"id": "pubmed23n0032_671", "title": "Changes of smoking habits and cough in men smoking cigarettes with 30% NSM tobacco substitute.", "content": "The effects of smoking cigarettes with 30% of the tobacco replaced by NSM tobacco substitute, which lowered their tar and nicotine delivery, were studied by comparing them with the effects of conventional cigarettes in a controlled crossover trial lasting 20 months. Chest symptoms, cigarette consumption, and forced expiratory volume in one second (FEV1) were measured each month. Two-hundred men began the trial and 159 completed it. The test cigarettes were acceptable to all but one of the men. In a subsample of 35 men estimates of nicotine intake were obtained from monthly analyses of cigarette stubs. On changing from NSM to control cigarettes six of the 17 men, who were accustomed to low nicotine, kept their nicotine intake down by some change in smoking habit. Before the crossover and this change in smoking habit the men smoking NSM cigarettes had a small but significant reduction of cough. Cigarettes containing 30% NSM and delivering only 1 mg of nicotine are likely to be acceptable to smokers and may reduce coughing. Further trials are needed to confirm these findings and establish what long-term effects such cigarettes may have on smokers' health.", "contents": "Changes of smoking habits and cough in men smoking cigarettes with 30% NSM tobacco substitute. The effects of smoking cigarettes with 30% of the tobacco replaced by NSM tobacco substitute, which lowered their tar and nicotine delivery, were studied by comparing them with the effects of conventional cigarettes in a controlled crossover trial lasting 20 months. Chest symptoms, cigarette consumption, and forced expiratory volume in one second (FEV1) were measured each month. Two-hundred men began the trial and 159 completed it. The test cigarettes were acceptable to all but one of the men. In a subsample of 35 men estimates of nicotine intake were obtained from monthly analyses of cigarette stubs. On changing from NSM to control cigarettes six of the 17 men, who were accustomed to low nicotine, kept their nicotine intake down by some change in smoking habit. Before the crossover and this change in smoking habit the men smoking NSM cigarettes had a small but significant reduction of cough. Cigarettes containing 30% NSM and delivering only 1 mg of nicotine are likely to be acceptable to smokers and may reduce coughing. Further trials are needed to confirm these findings and establish what long-term effects such cigarettes may have on smokers' health.", "PMID": 953529}
{"id": "pubmed23n0032_672", "title": "Low-tar medium-nicotine cigarettes: a new approach to safer smoking.", "content": "The logic of expecting people who cannot stop smoking to switch to cigarettes that have hardly any nicotine is questionable. Tar and nicotine yields of cigarettes available in Britain today correlate 0-93, and further reduction of tar intake is limited by the reluctance of smokers to tolerate similar reductions in nicotine. A new approach would be to aim at lowering tar yields of cigarettes from the present average of 18 mg to around 6 mg but maintaining nicotine yields at around 1-0 to 1-2 mg, which would be acceptable to most smokers. This approach requires that emphasis be placed on tar: nicotine ratios as well as on the absolute yields. These ratios for brands on sale in Britain today average 14-2 and range from 9-6 to 20-8. They provide an additional guide for comparing the relative harmfulness of different brands. For example, 35% of cigarette smokers in Britain smoke either Embassy Filter or Players No 6 Filter; by changing to John Player Carlton King Size they could reduce their tar intake by more than 20% without having to suffer any nicotine deprivation.", "contents": "Low-tar medium-nicotine cigarettes: a new approach to safer smoking. The logic of expecting people who cannot stop smoking to switch to cigarettes that have hardly any nicotine is questionable. Tar and nicotine yields of cigarettes available in Britain today correlate 0-93, and further reduction of tar intake is limited by the reluctance of smokers to tolerate similar reductions in nicotine. A new approach would be to aim at lowering tar yields of cigarettes from the present average of 18 mg to around 6 mg but maintaining nicotine yields at around 1-0 to 1-2 mg, which would be acceptable to most smokers. This approach requires that emphasis be placed on tar: nicotine ratios as well as on the absolute yields. These ratios for brands on sale in Britain today average 14-2 and range from 9-6 to 20-8. They provide an additional guide for comparing the relative harmfulness of different brands. For example, 35% of cigarette smokers in Britain smoke either Embassy Filter or Players No 6 Filter; by changing to John Player Carlton King Size they could reduce their tar intake by more than 20% without having to suffer any nicotine deprivation.", "PMID": 953530}
{"id": "pubmed23n0032_673", "title": "Ultrasonic monitoring of fetal respiratory movement.", "content": "Ultrasonic monitoring of fetal respiratory movement including velocity measurements is described. Both proximal and distal walls of the fetal chest are imaged simultaneously. The time-position mode clearly defines fetal respiratory movement and rejects artefacts.", "contents": "Ultrasonic monitoring of fetal respiratory movement. Ultrasonic monitoring of fetal respiratory movement including velocity measurements is described. Both proximal and distal walls of the fetal chest are imaged simultaneously. The time-position mode clearly defines fetal respiratory movement and rejects artefacts.", "PMID": 953531}
{"id": "pubmed23n0032_674", "title": "Carpal tunnel syndrome, humeral epicondylitis, and the cervical spine: a study of clinical and dimensional relations.", "content": "Forty-three patients with idiopathic carpal tunnel syndrome, confirmed by nerve conduction studies and treated by surgery, were compared clinically and radiologically with 43 age- and sex-matched control patients. Patients with carpal tunnel syndrome had a significantly greater prevalence of lateral humeral epicondylitis (tennis elbow) (33%) than controls (7%). Randomised reading of the cervical spine radiographs in ignorance of the groups to which they belonged showed no significant difference in the prevalence of either intervertebral disc degeneration or intraforaminal osteophyte protruion using conventional grading methods. Measurement of the minimum anteroposterior diameter of the cervical spinal canal, the anteroposterior diameters of the cervical vertebral bodies, and the ratio of intervertebral disc height to adjacent vertebral body height in the cervical spine, however, showed a consistent trend to smaller measurements in the carpal tunnel group. Differences were significant at several vertebral levels in each of these dimensions. The narrowing of the intervertebral discs relative to the vertebral bodies in patients with carpal tunnel syndrome may indicate connective tissue changes, which might also occur in the common extensor origin at the elbow or in the contents of the carpal tunnel.", "contents": "Carpal tunnel syndrome, humeral epicondylitis, and the cervical spine: a study of clinical and dimensional relations. Forty-three patients with idiopathic carpal tunnel syndrome, confirmed by nerve conduction studies and treated by surgery, were compared clinically and radiologically with 43 age- and sex-matched control patients. Patients with carpal tunnel syndrome had a significantly greater prevalence of lateral humeral epicondylitis (tennis elbow) (33%) than controls (7%). Randomised reading of the cervical spine radiographs in ignorance of the groups to which they belonged showed no significant difference in the prevalence of either intervertebral disc degeneration or intraforaminal osteophyte protruion using conventional grading methods. Measurement of the minimum anteroposterior diameter of the cervical spinal canal, the anteroposterior diameters of the cervical vertebral bodies, and the ratio of intervertebral disc height to adjacent vertebral body height in the cervical spine, however, showed a consistent trend to smaller measurements in the carpal tunnel group. Differences were significant at several vertebral levels in each of these dimensions. The narrowing of the intervertebral discs relative to the vertebral bodies in patients with carpal tunnel syndrome may indicate connective tissue changes, which might also occur in the common extensor origin at the elbow or in the contents of the carpal tunnel.", "PMID": 953532}
{"id": "pubmed23n0032_675", "title": "Management of psychiatric problems in a Kenyan mission hospital.", "content": "Many psychiatric conditions are found in the rural general hospital in Kenya and are recognisable and manageable along standard Western lines. An essential element in management is the training of the nursing staff. The psychiatric patient should be actively managed and can benefit from the unsophisticated treatment possible at the mission hospital.", "contents": "Management of psychiatric problems in a Kenyan mission hospital. Many psychiatric conditions are found in the rural general hospital in Kenya and are recognisable and manageable along standard Western lines. An essential element in management is the training of the nursing staff. The psychiatric patient should be actively managed and can benefit from the unsophisticated treatment possible at the mission hospital.", "PMID": 953535}
{"id": "pubmed23n0032_676", "title": "Breast-feeding protects against respiratory syncytial virus infections.", "content": "Eight out of 115 infants admitted to hospital with respiratory syncytial (RS) virus infection had been breast-fed compared with 46 out of 167 controls; this difference was statistically significant. Twenty-one specimens of human colostrum were examined, and all contained RS virus neutralising activity. Specific IgA and IgG were detected in 18 specimens, whereas IgM was detected in none. The titre of IgA antibody was usually higher and correlated more closely to the titre of neutralising activity than that of IgG. Infants inhale milk feeds and regurgitate them through the nose, and the IgA collecting in the respiratory tract might protect against severe respiratory infection. Alternatively, if severe RS virus illness is a sign of hypersensitivity to the virus breast-feeding might protect the infant from an early sensitising infection.", "contents": "Breast-feeding protects against respiratory syncytial virus infections. Eight out of 115 infants admitted to hospital with respiratory syncytial (RS) virus infection had been breast-fed compared with 46 out of 167 controls; this difference was statistically significant. Twenty-one specimens of human colostrum were examined, and all contained RS virus neutralising activity. Specific IgA and IgG were detected in 18 specimens, whereas IgM was detected in none. The titre of IgA antibody was usually higher and correlated more closely to the titre of neutralising activity than that of IgG. Infants inhale milk feeds and regurgitate them through the nose, and the IgA collecting in the respiratory tract might protect against severe respiratory infection. Alternatively, if severe RS virus illness is a sign of hypersensitivity to the virus breast-feeding might protect the infant from an early sensitising infection.", "PMID": 953560}
{"id": "pubmed23n0032_677", "title": "Lymphocyte cytotoxicity for kidney cells in renal tubular acidosis of autoimmune liver disease.", "content": "Sensitisation to a renal tubular antigen, Tamm-Horsfall glycoprotein, has been shown to be common in patients with renal tubular acidosis complicating autoimmune liver disease, and it has been suggested that this immune reaction, by damaging renal tubular cells, might be responsible for the acidification defect. The lymphocytes from 10 out of 13 patients with chronic active hepatitis or primary biliary cirrhosis and an associated renal tubular acidosis were shown to be cytotoxic for a kidney cell line known to secrete Tamm-Horsfall glycoprotein. The cytotoxic reaction was blocked by this antigen, but not by two other proteins, indicating that sensitisation to the renal glycoprotein was the likely cause of the target cell damage. Significant reduction in cytotoxicity after the addition of aggregated IgG suggested that the reaction was of the antibody-dependent cell-mediated type. These results, together with the finding of antigenic material in the surface membrane of liver cells that cross reacts immunologically with Tamm-Horsfall glycoprotein, provide an explanation for the association between chronic liver disease and renal tubular dysfunction.", "contents": "Lymphocyte cytotoxicity for kidney cells in renal tubular acidosis of autoimmune liver disease. Sensitisation to a renal tubular antigen, Tamm-Horsfall glycoprotein, has been shown to be common in patients with renal tubular acidosis complicating autoimmune liver disease, and it has been suggested that this immune reaction, by damaging renal tubular cells, might be responsible for the acidification defect. The lymphocytes from 10 out of 13 patients with chronic active hepatitis or primary biliary cirrhosis and an associated renal tubular acidosis were shown to be cytotoxic for a kidney cell line known to secrete Tamm-Horsfall glycoprotein. The cytotoxic reaction was blocked by this antigen, but not by two other proteins, indicating that sensitisation to the renal glycoprotein was the likely cause of the target cell damage. Significant reduction in cytotoxicity after the addition of aggregated IgG suggested that the reaction was of the antibody-dependent cell-mediated type. These results, together with the finding of antigenic material in the surface membrane of liver cells that cross reacts immunologically with Tamm-Horsfall glycoprotein, provide an explanation for the association between chronic liver disease and renal tubular dysfunction.", "PMID": 953561}
{"id": "pubmed23n0032_678", "title": "Oral contraceptive use in older women and fatal myocardial infarction.", "content": "A previous study of women who had died from myocardial infarction and of a control group of women matched with them for age suggested a fivefold increase in the risk of death from myocardial infarction among users of oral contraceptive aged 40-44 years compared with women not using such preparations. Only a small proportion of women in the infarction and control groups had used oral contraceptives, however, so the margin of error was wide. We therefore investigated a further 54 women in this age group who died from myocardial infarction and compared their oral contraceptive histories with those of age-matched, living controls. Combination of the findings from the present investigation with the previous results have enabled a revised estimate of a threefold increase in risk to be made. Although this risk estimate is similar to that previously shown for a younger age group, the total mortality attributable to complications associated with the use of oral contraceptives remained considerably greater among women over the age of 40.", "contents": "Oral contraceptive use in older women and fatal myocardial infarction. A previous study of women who had died from myocardial infarction and of a control group of women matched with them for age suggested a fivefold increase in the risk of death from myocardial infarction among users of oral contraceptive aged 40-44 years compared with women not using such preparations. Only a small proportion of women in the infarction and control groups had used oral contraceptives, however, so the margin of error was wide. We therefore investigated a further 54 women in this age group who died from myocardial infarction and compared their oral contraceptive histories with those of age-matched, living controls. Combination of the findings from the present investigation with the previous results have enabled a revised estimate of a threefold increase in risk to be made. Although this risk estimate is similar to that previously shown for a younger age group, the total mortality attributable to complications associated with the use of oral contraceptives remained considerably greater among women over the age of 40.", "PMID": 953602}
{"id": "pubmed23n0032_679", "title": "First electrocardiogram in recent myocardial infarction.", "content": "The admission electrocardiogram (ECG) was studied in 898 patients admitted to a coronary care unit over two years. The diagnosis made from this tracing was compared with that made at the end of the patient's stay. About half the cases of recent myocardial infarct were diagnosed from the admission ECG, but accuracy rose to 83% with serial ECG's in the unit. The ECG is important but not entirely reliable in the early detection of acute myocardial infarction, which should be largely a clinical diagnosis.", "contents": "First electrocardiogram in recent myocardial infarction. The admission electrocardiogram (ECG) was studied in 898 patients admitted to a coronary care unit over two years. The diagnosis made from this tracing was compared with that made at the end of the patient's stay. About half the cases of recent myocardial infarct were diagnosed from the admission ECG, but accuracy rose to 83% with serial ECG's in the unit. The ECG is important but not entirely reliable in the early detection of acute myocardial infarction, which should be largely a clinical diagnosis.", "PMID": 953603}
{"id": "pubmed23n0032_680", "title": "The elderly in a coronary unit.", "content": "The clinical features of myocardial infarction were compared in 104 patients over the age of 70 and 399 younger patients admitted to a coronary care unit. Absence of an age bar at 70 years has increased the number of admission to the unit by 24%, and the number of patients with proved infarcts by 26%. Severe complications are more common and mortality is doubled in the elderly. Although immediate management of primary ventricular fibrillation is as successful in older as in younger patients, treatment of the elderly with less dramatic conditions is less successful. The elderly survivors tend to spend longer in the coronary unit and subsequently in the general medical ward.", "contents": "The elderly in a coronary unit. The clinical features of myocardial infarction were compared in 104 patients over the age of 70 and 399 younger patients admitted to a coronary care unit. Absence of an age bar at 70 years has increased the number of admission to the unit by 24%, and the number of patients with proved infarcts by 26%. Severe complications are more common and mortality is doubled in the elderly. Although immediate management of primary ventricular fibrillation is as successful in older as in younger patients, treatment of the elderly with less dramatic conditions is less successful. The elderly survivors tend to spend longer in the coronary unit and subsequently in the general medical ward.", "PMID": 953604}
{"id": "pubmed23n0032_681", "title": "Value of renal biopsy in acute intrinsic renal failure.", "content": "Eighty-four patients presented with acute renal failure and features suggesting a diagnosis of intrinsic renal disease other than \"acute reversible renal failure.\" Renal biopsy proved valuable in establishing the diagnosis, in indicating the reversibility of the lesion, and in helping to decide on treatment.", "contents": "Value of renal biopsy in acute intrinsic renal failure. Eighty-four patients presented with acute renal failure and features suggesting a diagnosis of intrinsic renal disease other than \"acute reversible renal failure.\" Renal biopsy proved valuable in establishing the diagnosis, in indicating the reversibility of the lesion, and in helping to decide on treatment.", "PMID": 953608}
{"id": "pubmed23n0032_682", "title": "Cost of management of patients with haemophilia.", "content": "The cost of managing 114 adult haemophiliacs in the west of Scotland was assessed for the period 1 March 1971 to 28 February 1974. Altogether 23 of them (20%) accounted for 80% of the resources used. The cost of hospital treatment of these patients during the period was compared with the predicted cost of home treatment, given the availability of freeze-dried factor VIII concentrate in sufficient amounts. We calculate that adequate on-demand home treatment would cost only 16% more than the present treatment, which is substantially less efficient.", "contents": "Cost of management of patients with haemophilia. The cost of managing 114 adult haemophiliacs in the west of Scotland was assessed for the period 1 March 1971 to 28 February 1974. Altogether 23 of them (20%) accounted for 80% of the resources used. The cost of hospital treatment of these patients during the period was compared with the predicted cost of home treatment, given the availability of freeze-dried factor VIII concentrate in sufficient amounts. We calculate that adequate on-demand home treatment would cost only 16% more than the present treatment, which is substantially less efficient.", "PMID": 953611}
{"id": "pubmed23n0032_683", "title": "Mountain sickness, retinal haemorrhages, and acclimatisation on Mount Everest in 1975.", "content": "During the 1975 British Everest Expedition, which made the first ascent of the south-west face, observations were made in relation to mountain sickness and the appearance of retinal changes. Two Sherpas with cerebral oedema and one Briton with pulmonary oedema were treated. Retinal haemorrhages occurred in four out of six Britons who were newcomers to altitudes over 6000 m (19 685 ft) but in only two out of 14 Britons who had previously visited these altitudes. Intraocular pressures during ascent to 6000 m were within normal limits. The relevance of the ocular findings to acclimatisation in previous years was examined, the results supporting the hypothesis of a \"carry-over\" effect from previous visits to high altitude.", "contents": "Mountain sickness, retinal haemorrhages, and acclimatisation on Mount Everest in 1975. During the 1975 British Everest Expedition, which made the first ascent of the south-west face, observations were made in relation to mountain sickness and the appearance of retinal changes. Two Sherpas with cerebral oedema and one Briton with pulmonary oedema were treated. Retinal haemorrhages occurred in four out of six Britons who were newcomers to altitudes over 6000 m (19 685 ft) but in only two out of 14 Britons who had previously visited these altitudes. Intraocular pressures during ascent to 6000 m were within normal limits. The relevance of the ocular findings to acclimatisation in previous years was examined, the results supporting the hypothesis of a \"carry-over\" effect from previous visits to high altitude.", "PMID": 953646}
{"id": "pubmed23n0032_684", "title": "Postoperative hypoglycaemia in small children.", "content": "Six of 57 children under 4 years old became hypoglycaemic during postoperative recovery from various surgical procedures. Fluids either by mouth or intravenously did not always prevent this. Children at risk could not be predicted on the basis of age or weight-for-age. Small children should be fed as soon as practicable after operations, preferably with milk.", "contents": "Postoperative hypoglycaemia in small children. Six of 57 children under 4 years old became hypoglycaemic during postoperative recovery from various surgical procedures. Fluids either by mouth or intravenously did not always prevent this. Children at risk could not be predicted on the basis of age or weight-for-age. Small children should be fed as soon as practicable after operations, preferably with milk.", "PMID": 953647}
{"id": "pubmed23n0032_685", "title": "Completeness of statutory notification for acute bacterial meningitis.", "content": "Several different sources of data, including statutory notifications, were used to identify cases of acute bacterial meningitis in childhood in a defined population. Only half the cases of meningococcal meningitis and less than one quarter of ofther types of bacterial meningitis had been notified. Notified cases differed from the remainder with respect to causative organism, age, outcome, and type of hospital of admission. Thus notifications are an unreliable measure of incidence for this disease, and studies of infectious disease based on notified cases alone should be interpreted with considerable reserve. It is suggested that infectious-disease surveillance should be both clinical and microbiological and that data from different sources need to co-ordinated. The reason for reporting each disease (or organism) should be specified and and reviewed periodically.", "contents": "Completeness of statutory notification for acute bacterial meningitis. Several different sources of data, including statutory notifications, were used to identify cases of acute bacterial meningitis in childhood in a defined population. Only half the cases of meningococcal meningitis and less than one quarter of ofther types of bacterial meningitis had been notified. Notified cases differed from the remainder with respect to causative organism, age, outcome, and type of hospital of admission. Thus notifications are an unreliable measure of incidence for this disease, and studies of infectious disease based on notified cases alone should be interpreted with considerable reserve. It is suggested that infectious-disease surveillance should be both clinical and microbiological and that data from different sources need to co-ordinated. The reason for reporting each disease (or organism) should be specified and and reviewed periodically.", "PMID": 953648}
{"id": "pubmed23n0032_686", "title": "A general practitioner in an ophthalmology accident and emergency department.", "content": "After a short period of intensive training, a general practitioner successfully replaced a senior house officer (SHO) in the accident and emergency department of an eye hospital on one morning a week for a year. An unbiased observer compared the performance of the general practitioner after one year with that of a full-time SHO who had had 17 months' experience; their performances were about equal. Although a sessional general practitioner costs about 28% more than an SHO, the real cost is much less because undue length of service as an SHO or change to another specialty (because of the SHO surplus) delays achievement of a permanent grade. Continuity is a great advantage of the general practitioner. Replacement of some SHOs by general practitioners would reduce the surplus of SHOs with poor promotion prospects. The commonest diagnoses were Meibomian cysts (18%), corneal foreign bodies (20%), corneal abrasions (12%), and conjunctivitis (8%).", "contents": "A general practitioner in an ophthalmology accident and emergency department. After a short period of intensive training, a general practitioner successfully replaced a senior house officer (SHO) in the accident and emergency department of an eye hospital on one morning a week for a year. An unbiased observer compared the performance of the general practitioner after one year with that of a full-time SHO who had had 17 months' experience; their performances were about equal. Although a sessional general practitioner costs about 28% more than an SHO, the real cost is much less because undue length of service as an SHO or change to another specialty (because of the SHO surplus) delays achievement of a permanent grade. Continuity is a great advantage of the general practitioner. Replacement of some SHOs by general practitioners would reduce the surplus of SHOs with poor promotion prospects. The commonest diagnoses were Meibomian cysts (18%), corneal foreign bodies (20%), corneal abrasions (12%), and conjunctivitis (8%).", "PMID": 953652}
{"id": "pubmed23n0032_687", "title": "Emergency medical care.", "content": "A survey carried out over five periods between 1973 and 1975 to study the mode of referral of emergency medical patients to a district general hospital showed that, out of a total of 2511 patients, 51% referred themselves, 40-8% were referred by general practitioners, and only 4-7% by doctors employed by the emergency treatment service. Of the 1720 patients admitted to the medical wards, 50-9% were referred by general practitioners and 37-3% were self-referred while the corresponding figures for the 791 not admitted were 19% and 80-7% respectively. Two-thirds of the self-referred patients came from their own homes, usually by ambulance ordered by a \"999\" emergency call. The figures were similar in each of the five periods.", "contents": "Emergency medical care. A survey carried out over five periods between 1973 and 1975 to study the mode of referral of emergency medical patients to a district general hospital showed that, out of a total of 2511 patients, 51% referred themselves, 40-8% were referred by general practitioners, and only 4-7% by doctors employed by the emergency treatment service. Of the 1720 patients admitted to the medical wards, 50-9% were referred by general practitioners and 37-3% were self-referred while the corresponding figures for the 791 not admitted were 19% and 80-7% respectively. Two-thirds of the self-referred patients came from their own homes, usually by ambulance ordered by a \"999\" emergency call. The figures were similar in each of the five periods.", "PMID": 953653}
{"id": "pubmed23n0032_688", "title": "[Pathways and ascending vestibular projections emanating from primary nuclei: radioautographic study (author's transl)].", "content": "A study of the pathways and ascending vestibular projections was carried out in the cat after unilateral injection of tritiated leucine into the rostral vestibular complex. Radioautographic analysis revealed a gradual decline in the density of labeling as ascending fibers were found to progress towards more rostral relays. The pathways and projections were very compact in the oculomotor nuclei, became less intense in the Cajal and Darkschewitsch nuclei, and thinned out considerably until they reached a transitional zone in the thalamus between the ventrobasal and ventrolateral complexes. These results confirm established and previous findings in this laboratory obtained by neurophysiological and neurohistological examination procedures. They provide the first anatomical evidence concerning the existence of vestibulothalamic projections and pathways.", "contents": "[Pathways and ascending vestibular projections emanating from primary nuclei: radioautographic study (author's transl)]. A study of the pathways and ascending vestibular projections was carried out in the cat after unilateral injection of tritiated leucine into the rostral vestibular complex. Radioautographic analysis revealed a gradual decline in the density of labeling as ascending fibers were found to progress towards more rostral relays. The pathways and projections were very compact in the oculomotor nuclei, became less intense in the Cajal and Darkschewitsch nuclei, and thinned out considerably until they reached a transitional zone in the thalamus between the ventrobasal and ventrolateral complexes. These results confirm established and previous findings in this laboratory obtained by neurophysiological and neurohistological examination procedures. They provide the first anatomical evidence concerning the existence of vestibulothalamic projections and pathways.", "PMID": 953689}
{"id": "pubmed23n0032_689", "title": "GM2 ganglioside in fetal Tay-Sachs disease brain cultures: a model system for the disease.", "content": "Cultured cells derived from Tay-Sachs disease (TSD) fetal cerebellum were shown to accumulate GM2 ganglioside when compared with control cultures. In contrast, fibroblasts derived from TSD fetal lung do not contain GM2. GM2 was identified by thin-layer chromatography (TLC) and confirmed by gas-liquid chromatography (GLC). Unlike fetal TSD brain, the cell cultures established from fetal TSD brain have high concentrations of globoside and GD3 and little or no asialo GM2(GA2). The GM2 and related glycosphingolipids in these cultured cells contain a high percentage of C24:0 and C24:1 fatty acids. In spite of these differences, this TSD cell strain is unique in that it accumulates GM2, and can therefore serve as a useful in vitro model for the study of TSD.", "contents": "GM2 ganglioside in fetal Tay-Sachs disease brain cultures: a model system for the disease. Cultured cells derived from Tay-Sachs disease (TSD) fetal cerebellum were shown to accumulate GM2 ganglioside when compared with control cultures. In contrast, fibroblasts derived from TSD fetal lung do not contain GM2. GM2 was identified by thin-layer chromatography (TLC) and confirmed by gas-liquid chromatography (GLC). Unlike fetal TSD brain, the cell cultures established from fetal TSD brain have high concentrations of globoside and GD3 and little or no asialo GM2(GA2). The GM2 and related glycosphingolipids in these cultured cells contain a high percentage of C24:0 and C24:1 fatty acids. In spite of these differences, this TSD cell strain is unique in that it accumulates GM2, and can therefore serve as a useful in vitro model for the study of TSD.", "PMID": 953690}
{"id": "pubmed23n0032_690", "title": "Neurochemical aspects of the ontogenesis of GABAnergic neurons in the rat brain.", "content": "Examination of various biochemical characteristics of the GABAnergic nervous system in the rat brain was made between 15 days of gestation and adulthood. At birth, the concentration of GABA in whole brain and most regions is approximately 50% of adult levels, whereas the medulla-pons has achieved adult levels by this time. Compared to GABA levels, there is a marked lag in the development of the activity of glutamic acid decarboxylase in all areas studied; however, the activity of the enzyme increases in a linear fashion from birth to adulthood. The development of the uptake of GABA into particulate fractions prepared from whole brain and regions differs markedly from that of GABA and glutamic acid decarboxylase, with uptake near adult levels by birth, peaking considerably above that of the adult between one to two weeks after birth and then declining toward adult activity by 4 weeks after birth. Examination of the kinetics of GABA uptake into resuspended P2 fractions demonstrates that the developmental changes in the uptake reflects differences in the Vmax whereas the Kt remains constant; Studies on the development of the apparent postsynaptic receptor for GABA reveals that in all regions binding is approximately 25% of adult up to 8 days after birth, at which time it increases dramatically, approximating adult levels by 4 weeks after birth. The rise in the density of the apparent postsynaptic GABA receptor after 8 days postpartum correlates best with the increase in the activity of glutamic acid decarboxylase, a presynaptic marker.", "contents": "Neurochemical aspects of the ontogenesis of GABAnergic neurons in the rat brain. Examination of various biochemical characteristics of the GABAnergic nervous system in the rat brain was made between 15 days of gestation and adulthood. At birth, the concentration of GABA in whole brain and most regions is approximately 50% of adult levels, whereas the medulla-pons has achieved adult levels by this time. Compared to GABA levels, there is a marked lag in the development of the activity of glutamic acid decarboxylase in all areas studied; however, the activity of the enzyme increases in a linear fashion from birth to adulthood. The development of the uptake of GABA into particulate fractions prepared from whole brain and regions differs markedly from that of GABA and glutamic acid decarboxylase, with uptake near adult levels by birth, peaking considerably above that of the adult between one to two weeks after birth and then declining toward adult activity by 4 weeks after birth. Examination of the kinetics of GABA uptake into resuspended P2 fractions demonstrates that the developmental changes in the uptake reflects differences in the Vmax whereas the Kt remains constant; Studies on the development of the apparent postsynaptic receptor for GABA reveals that in all regions binding is approximately 25% of adult up to 8 days after birth, at which time it increases dramatically, approximating adult levels by 4 weeks after birth. The rise in the density of the apparent postsynaptic GABA receptor after 8 days postpartum correlates best with the increase in the activity of glutamic acid decarboxylase, a presynaptic marker.", "PMID": 953691}
{"id": "pubmed23n0032_691", "title": "[Postnatal maturation of vestibular nuclei in the cat: histological study by the Golgi-Cox method (author's transl)].", "content": "Postnatal maturation of vestibular nuclei in the cat was studied from both the qualitative and quantitative points of view, following impregnation of the sections by the Golgi-Cox method. Three principal data arise from our results. (1) The different vestibular neurones undergo significant growth during the first two weeks of extra-uterine life: during this time, the progressive disappearance of dendritic growth cones and filopodia, an increase in area and volume of the perikarya as well as that of dendritic fields are remarkable; the smallest neurones completing their maturation before the largest. (2) Average sized neurones of the superior vestibular nucleus show a significant increase in the number of dendritic spines between birth and the age of 3 days. (3) On the 12th day, a decrease in the number of dendritic spines is noted. This decrease is accompagnied by a modification in spine density along the dendrites.", "contents": "[Postnatal maturation of vestibular nuclei in the cat: histological study by the Golgi-Cox method (author's transl)]. Postnatal maturation of vestibular nuclei in the cat was studied from both the qualitative and quantitative points of view, following impregnation of the sections by the Golgi-Cox method. Three principal data arise from our results. (1) The different vestibular neurones undergo significant growth during the first two weeks of extra-uterine life: during this time, the progressive disappearance of dendritic growth cones and filopodia, an increase in area and volume of the perikarya as well as that of dendritic fields are remarkable; the smallest neurones completing their maturation before the largest. (2) Average sized neurones of the superior vestibular nucleus show a significant increase in the number of dendritic spines between birth and the age of 3 days. (3) On the 12th day, a decrease in the number of dendritic spines is noted. This decrease is accompagnied by a modification in spine density along the dendrites.", "PMID": 953692}
{"id": "pubmed23n0032_692", "title": "Binding of alpha-bungarotoxin to chick sympathetic ganglia: properties of the receptor and its rate of appearance during developement.", "content": "Studies were carried out on the binding of [125I]alpha-bungarotoxin (alphaBT) to membrane fragments of chick sympathetic ganglia. Specific binding of toxin was saturable with a KD of 1.1 nM. The rates of association and dissociation of the toxin from ganglionic membranes were 4.3 X 10(4) M-1 sec-1 and 4.6 X 10(-5) sec-1 (t 1/2 = 4.2 h). respectively. Binding was inhibited (by up to 95%) by low concentrations of nicotinic, but not by a muscarinic cholinergic ligand. The properties of the ganglionic binding site for alphaBT were consistent with its being a nicotinic acetylcholine receptor. The development of toxin receptors in chick ganglia was also studied. From days 7 to 11 in ovo, few receptors were present; from days 12 to 20 in ovo, there was a 10-fold increase in receptor number per ganglion; from hatching to maturity, the receptor number per ganglion slowly increased and reached a maximum of 14 fmoles. The ontogeny of receptors for alphaBT in sympathetic ganglia appears to correlate with the cytological maturation and innervation of the principal neurons.", "contents": "Binding of alpha-bungarotoxin to chick sympathetic ganglia: properties of the receptor and its rate of appearance during developement. Studies were carried out on the binding of [125I]alpha-bungarotoxin (alphaBT) to membrane fragments of chick sympathetic ganglia. Specific binding of toxin was saturable with a KD of 1.1 nM. The rates of association and dissociation of the toxin from ganglionic membranes were 4.3 X 10(4) M-1 sec-1 and 4.6 X 10(-5) sec-1 (t 1/2 = 4.2 h). respectively. Binding was inhibited (by up to 95%) by low concentrations of nicotinic, but not by a muscarinic cholinergic ligand. The properties of the ganglionic binding site for alphaBT were consistent with its being a nicotinic acetylcholine receptor. The development of toxin receptors in chick ganglia was also studied. From days 7 to 11 in ovo, few receptors were present; from days 12 to 20 in ovo, there was a 10-fold increase in receptor number per ganglion; from hatching to maturity, the receptor number per ganglion slowly increased and reached a maximum of 14 fmoles. The ontogeny of receptors for alphaBT in sympathetic ganglia appears to correlate with the cytological maturation and innervation of the principal neurons.", "PMID": 953693}
{"id": "pubmed23n0032_693", "title": "The head extractions, brain and pituitary uptake and metabolism of progesterone and 5alpha-dihydroprogesterone in anesthetized, female rabbits.", "content": "Three days after ovariectomy adult female rabbits were injected intramuscularly with either 2 mug estradiol/kg b.w., 0.5 mg progesterone/kg b.w., 2 mug estradiol plus 0.5 mg progesterone/kg b.w., or oil vehicle daily for 5 days. On the sixth day the animals were anesthetized and given a continuous infusion of [14C]progesterone and [3H]5alpha-pregnane-3,20-dione (5alpha-DHP) for 4 h via the femoral vein. Head extractions, calculated from the difference in blood concentrations of radioactive steriods between the femoral artery and jugular vein, were in the range of 60-75% for both progesterone and 5alpha-DHP and were unaffected by the hormone treatments. Nine brain areas and the pituitary were analyzed for [14C]progesterone, [3H]5alpha-DHP and [14C]-5alpha-DHP. Generally, the brain and pituitary retention and distribution of [14C]progesterone and [3H]5alpha-DHP and the brain and pituitary metabolism of [14C]progesterone were unaffected by hormone treatments. In the cerebellum, progesterone treatment increased [14C]progesterone retention as compared to oil treatment. All tissues contained 2-7 times more [14C]progesterone than arterial blood concentrations. [3H]5alpha-DHP tissue: arterial blood concentrations ratios were much lower ranging from 0.9 to 1.78. [14C]progesterone and [3H]5alpha-DHP showed similar brain distribution with highest concentrations in the pons, pons reticulum and midbrain reticulum. All tissues converted progesterone to 5alpha-DHP, but only the cerebellum contained more [14C]5alpha-DHP than [14C]progesterone.", "contents": "The head extractions, brain and pituitary uptake and metabolism of progesterone and 5alpha-dihydroprogesterone in anesthetized, female rabbits. Three days after ovariectomy adult female rabbits were injected intramuscularly with either 2 mug estradiol/kg b.w., 0.5 mg progesterone/kg b.w., 2 mug estradiol plus 0.5 mg progesterone/kg b.w., or oil vehicle daily for 5 days. On the sixth day the animals were anesthetized and given a continuous infusion of [14C]progesterone and [3H]5alpha-pregnane-3,20-dione (5alpha-DHP) for 4 h via the femoral vein. Head extractions, calculated from the difference in blood concentrations of radioactive steriods between the femoral artery and jugular vein, were in the range of 60-75% for both progesterone and 5alpha-DHP and were unaffected by the hormone treatments. Nine brain areas and the pituitary were analyzed for [14C]progesterone, [3H]5alpha-DHP and [14C]-5alpha-DHP. Generally, the brain and pituitary retention and distribution of [14C]progesterone and [3H]5alpha-DHP and the brain and pituitary metabolism of [14C]progesterone were unaffected by hormone treatments. In the cerebellum, progesterone treatment increased [14C]progesterone retention as compared to oil treatment. All tissues contained 2-7 times more [14C]progesterone than arterial blood concentrations. [3H]5alpha-DHP tissue: arterial blood concentrations ratios were much lower ranging from 0.9 to 1.78. [14C]progesterone and [3H]5alpha-DHP showed similar brain distribution with highest concentrations in the pons, pons reticulum and midbrain reticulum. All tissues converted progesterone to 5alpha-DHP, but only the cerebellum contained more [14C]5alpha-DHP than [14C]progesterone.", "PMID": 953694}
{"id": "pubmed23n0032_694", "title": "Inhibitory potentials produced in cortical cells by stimulation of the lateral hypothalamus in rabbits.", "content": "(1) Intracellular potentials were recorded from pyramidal tract (PT) and non-pyramidal tract (non-PT) cells of the frontal cortex in urethane-anesthetized rabbits and the effects of electrical stimulation of the lateral hypothalamus (LH) were examined on the ipsilateral side. (2) Latencies of antidromic spikes of PT cells evoked by stimulation of the medullary pyramidal tract (PYR) had a unimodal distribution with the mean at 4.0 msec. The mean conduction velocity of the pyramidal tract fibers was 10.5 m/sec. (3) Prolonged IPSPs were produced in PT and non-PT cells by single shock stimulation of LH (LH-IPSP). They were significantly longer lasting than those produced by PYR stimulation (PYR-IPSP). (4) The latencies of LH-IPSPs ranged from 1.7 to 25.0 msec and were divided into two groups. The latencies of PYR-IPSPs had a unimodal distribution ranging from 2.5 to 30 msec. (5) In a few non-PT cells, a sequence of brief depolarization and prolonged hyperpolarization occurred in response to LH stimulation. (6) In some non-PT cells, EPSPs which occasionally resulted in spike discharges were observed after LH stimulation. However, no excitation of PT cells was observed by LH stimulation.", "contents": "Inhibitory potentials produced in cortical cells by stimulation of the lateral hypothalamus in rabbits. (1) Intracellular potentials were recorded from pyramidal tract (PT) and non-pyramidal tract (non-PT) cells of the frontal cortex in urethane-anesthetized rabbits and the effects of electrical stimulation of the lateral hypothalamus (LH) were examined on the ipsilateral side. (2) Latencies of antidromic spikes of PT cells evoked by stimulation of the medullary pyramidal tract (PYR) had a unimodal distribution with the mean at 4.0 msec. The mean conduction velocity of the pyramidal tract fibers was 10.5 m/sec. (3) Prolonged IPSPs were produced in PT and non-PT cells by single shock stimulation of LH (LH-IPSP). They were significantly longer lasting than those produced by PYR stimulation (PYR-IPSP). (4) The latencies of LH-IPSPs ranged from 1.7 to 25.0 msec and were divided into two groups. The latencies of PYR-IPSPs had a unimodal distribution ranging from 2.5 to 30 msec. (5) In a few non-PT cells, a sequence of brief depolarization and prolonged hyperpolarization occurred in response to LH stimulation. (6) In some non-PT cells, EPSPs which occasionally resulted in spike discharges were observed after LH stimulation. However, no excitation of PT cells was observed by LH stimulation.", "PMID": 953704}
{"id": "pubmed23n0032_695", "title": "Reticular suppression of flash-evoked IPSPs in visual cortex neurons.", "content": "In a majority of visual cortex (VC) neurons recorded intracellularly in chronically implanted rabbits, light flashes evoked responses consisting of protracted IPSPs (duration 160-200 msec) followed in some cases by a rebound. Inhibition of spontaneous activity through high frequency reticular stimulation (MRF) or through presentations of non-visual arousing stimuli, was not associated with strong hyperpolarization of the cell membrane. Reticular stimulation evoking both activation and inhibition of spontaneous neuronal activity elicited attenuation or complete elimination elimination (disinhibition) of flash-evoked protracted IPSPs and of the postinhibitory rebounds. Rhythmic neuronal discharges elicited by stimulation of the visual pathway was also reduced during reticular activation, due to attenuation of the IPSP-rebound sequence.", "contents": "Reticular suppression of flash-evoked IPSPs in visual cortex neurons. In a majority of visual cortex (VC) neurons recorded intracellularly in chronically implanted rabbits, light flashes evoked responses consisting of protracted IPSPs (duration 160-200 msec) followed in some cases by a rebound. Inhibition of spontaneous activity through high frequency reticular stimulation (MRF) or through presentations of non-visual arousing stimuli, was not associated with strong hyperpolarization of the cell membrane. Reticular stimulation evoking both activation and inhibition of spontaneous neuronal activity elicited attenuation or complete elimination elimination (disinhibition) of flash-evoked protracted IPSPs and of the postinhibitory rebounds. Rhythmic neuronal discharges elicited by stimulation of the visual pathway was also reduced during reticular activation, due to attenuation of the IPSP-rebound sequence.", "PMID": 953705}
{"id": "pubmed23n0032_696", "title": "Catecholamines released from cerebral cortex in the cat; decrease during sensory stimulation.", "content": "In an attempt to determine the functional role of catecholamine (CA) nerve terminals in cerebral cortex the release of endogenous norepinephrine (NE) and dopamine (DA) into superfusates from visual and somatosensory cortex of the cat have been measured by a sensitive radiometric enzymatic assay based on the methylation of CA by catechol-O-methyltransferase (COMT) in the presence of a [3H]-methyl donor and followed by resolution of 3H derivatives through a series of organic extractions. In the flaxedilized animal maintained under local anaesthesia with artificial respiration the concentration of CA measured in 30-min superfusates was fairly constant in a given experiment under basal conditions without sensory stimulation, but varied widely from one experiment to another. Variations in NE were often independent of those for DA. For visual cortex the average basal release of NE in experiments was 20.09 +/- 3.64 pg/min/sq.cm while the average for DA was 34.01 +/- 7.62 pg/min/sq.cm. In all experiments intermittent visual stimulation (15/sec) produced a significant reduction in release rate averaging about 42% for NE and 64% for DA in visual cortex. The reduction was relatively non-specific since visual or somatic sensory stimulation produced a decrease in release from both visual and somatic sensory cortical areas. Since it has been shown that there is a relatively non-specific increase in acetylcholine (ACh) release from sensory cortex during stimulation, it is proposed that ACh may regulate CA release at presynaptic CA terminals in the cortex as it does in the periphery. A marked increase in CA release observed on perfusing with nicotine or atropine is consistent with this hypothesis.", "contents": "Catecholamines released from cerebral cortex in the cat; decrease during sensory stimulation. In an attempt to determine the functional role of catecholamine (CA) nerve terminals in cerebral cortex the release of endogenous norepinephrine (NE) and dopamine (DA) into superfusates from visual and somatosensory cortex of the cat have been measured by a sensitive radiometric enzymatic assay based on the methylation of CA by catechol-O-methyltransferase (COMT) in the presence of a [3H]-methyl donor and followed by resolution of 3H derivatives through a series of organic extractions. In the flaxedilized animal maintained under local anaesthesia with artificial respiration the concentration of CA measured in 30-min superfusates was fairly constant in a given experiment under basal conditions without sensory stimulation, but varied widely from one experiment to another. Variations in NE were often independent of those for DA. For visual cortex the average basal release of NE in experiments was 20.09 +/- 3.64 pg/min/sq.cm while the average for DA was 34.01 +/- 7.62 pg/min/sq.cm. In all experiments intermittent visual stimulation (15/sec) produced a significant reduction in release rate averaging about 42% for NE and 64% for DA in visual cortex. The reduction was relatively non-specific since visual or somatic sensory stimulation produced a decrease in release from both visual and somatic sensory cortical areas. Since it has been shown that there is a relatively non-specific increase in acetylcholine (ACh) release from sensory cortex during stimulation, it is proposed that ACh may regulate CA release at presynaptic CA terminals in the cortex as it does in the periphery. A marked increase in CA release observed on perfusing with nicotine or atropine is consistent with this hypothesis.", "PMID": 953706}
{"id": "pubmed23n0032_697", "title": "The morphology of identified neurons in the abdominal ganglion of Aplysia californica.", "content": "The morphology of identified neurons and of one multiaction interneuron (L10) of the abdominal ganglion of Aplysia has been studied using cobalt chloride, injected intracellularly. Cells with little synaptic input, R3-R14, had a relatively poorly developed dendritic tree, whereas the dendrite tree of cells L7 and L10, with extensive synaptic input, was highly complex. Cells L1-L6 and the RB cell cluster were found to have intermediate complexity of synaptic inputs and dendritic morphology. Within cell clusters, individual cells were often morphologically distinct. Identified cells have both invariant and variant axonal branches. Variant axons often project down other than their customary nerve trunks or are supernumerary. Three features of neuropil architecture were encountered. (1) When cells from the same cluster send their axons down the same nerve the axons often run in fascicles. (2) Although an identified cell's dendritic geometry varies from preparation to preparation, its dendrites always occupy approximately the same position in the neuropil. (3) The postsynaptic follower cells of L10 send their main axons through the axonal arborization of L10.", "contents": "The morphology of identified neurons in the abdominal ganglion of Aplysia californica. The morphology of identified neurons and of one multiaction interneuron (L10) of the abdominal ganglion of Aplysia has been studied using cobalt chloride, injected intracellularly. Cells with little synaptic input, R3-R14, had a relatively poorly developed dendritic tree, whereas the dendrite tree of cells L7 and L10, with extensive synaptic input, was highly complex. Cells L1-L6 and the RB cell cluster were found to have intermediate complexity of synaptic inputs and dendritic morphology. Within cell clusters, individual cells were often morphologically distinct. Identified cells have both invariant and variant axonal branches. Variant axons often project down other than their customary nerve trunks or are supernumerary. Three features of neuropil architecture were encountered. (1) When cells from the same cluster send their axons down the same nerve the axons often run in fascicles. (2) Although an identified cell's dendritic geometry varies from preparation to preparation, its dendrites always occupy approximately the same position in the neuropil. (3) The postsynaptic follower cells of L10 send their main axons through the axonal arborization of L10.", "PMID": 953707}
{"id": "pubmed23n0032_698", "title": "A radioautographic analysis in the light and electron microscope of identified Aplysia neurons and their processes after intrasomatic injection of L-(3H)fucose.", "content": "We have studied radioautographically the distribution and fate of 3H-glycoproteins within the single identified neurons L10 and R12 of Aplysia californica after intrasomatic pressure injection of [3H]fucose. Silver grains were localized to intracytoplasmic membranes in both cell body and axon 3 h after injection of the cholinergic neuron L10. Grains also appear at this time over presumptive synapses. In the cell body the Golgi apparatus was labeled, as were vesicles, multivesicular bodies, pigment granules, smooth endoplasmic reticulum, mitochondria and peroxisomes. The Golgi apparatus is the most intensely labelled organelle (relative specific activity 9,5). Over 50% of the silver grains are associated with the Golgi apparatus and with vesicles. In the axons, vesicles were labeled most intensely, having a relative specific activity of 40.4 (% silver grains/% area), an intensity 10 times that of similar appearing somatic vesicles, and 4.5--10 times that of other organelles (multivesicular bodies, mitochondria, smooth endoplasmic reticulum) in the axon. At least 32% of the silver grains are associated with vesicles. It appears that the biosynthetic machinery of these neurons is heavily involved in the production of vesicle membrane destined for transport along axons and to terminals. The preponderant labeling of vesicles in the axon parallels the rapid and perferential transport of glycoprotein components described by Ambron et al. and may indicate that specific glycoprotein molecules can be identified as components of these vesicles. After injection of the cholinergic neuron R2 transport of radioactivity was restricted to the axonal tree of the injected neuron. After injection of L10, one other neuron was invariably labeled. By varying the conditions of injection, as many as 5 other neuron cell bodies could be labeled. These are located in the position of cells known to be electrically coupled to L10, and they probably became labeled by transneuronal movement of fucose across electrotonic junctions. Since restriction of label to the injected neuron is easily determined in each experiment, this technique makes possible the identification of chemical and perhaps electrical synapses of identified cells with optimal preservation of fine structure.", "contents": "A radioautographic analysis in the light and electron microscope of identified Aplysia neurons and their processes after intrasomatic injection of L-(3H)fucose. We have studied radioautographically the distribution and fate of 3H-glycoproteins within the single identified neurons L10 and R12 of Aplysia californica after intrasomatic pressure injection of [3H]fucose. Silver grains were localized to intracytoplasmic membranes in both cell body and axon 3 h after injection of the cholinergic neuron L10. Grains also appear at this time over presumptive synapses. In the cell body the Golgi apparatus was labeled, as were vesicles, multivesicular bodies, pigment granules, smooth endoplasmic reticulum, mitochondria and peroxisomes. The Golgi apparatus is the most intensely labelled organelle (relative specific activity 9,5). Over 50% of the silver grains are associated with the Golgi apparatus and with vesicles. In the axons, vesicles were labeled most intensely, having a relative specific activity of 40.4 (% silver grains/% area), an intensity 10 times that of similar appearing somatic vesicles, and 4.5--10 times that of other organelles (multivesicular bodies, mitochondria, smooth endoplasmic reticulum) in the axon. At least 32% of the silver grains are associated with vesicles. It appears that the biosynthetic machinery of these neurons is heavily involved in the production of vesicle membrane destined for transport along axons and to terminals. The preponderant labeling of vesicles in the axon parallels the rapid and perferential transport of glycoprotein components described by Ambron et al. and may indicate that specific glycoprotein molecules can be identified as components of these vesicles. After injection of the cholinergic neuron R2 transport of radioactivity was restricted to the axonal tree of the injected neuron. After injection of L10, one other neuron was invariably labeled. By varying the conditions of injection, as many as 5 other neuron cell bodies could be labeled. These are located in the position of cells known to be electrically coupled to L10, and they probably became labeled by transneuronal movement of fucose across electrotonic junctions. Since restriction of label to the injected neuron is easily determined in each experiment, this technique makes possible the identification of chemical and perhaps electrical synapses of identified cells with optimal preservation of fine structure.", "PMID": 953708}
{"id": "pubmed23n0032_699", "title": "Brain stem reticular units: synaptic responses to stimulation within the ascending reticular pathways.", "content": "When the ascending reticular axonal system is stimulated, the responses of distal structures (e.g., the cerebral cortex) appear to outlast the stimulus; these longlasting effects could reflect the intrinsic nature of the distal structure, or the response could reflect an interaction among the reticular cells which tends to prolong the effects of stimulation. To examine the latter hypothesis, single units with ascending axons (projecting units) were recorded in the cat rostral rhombencephalon in acute experiments conducted under halothane-nitrous oxide anesthesia. Stimulation of areas to or through which axons of reticular neurons projected (midbrain tegmentum and lower tectum, medial thalamus, and basal forebrain) produced a consistent and specific response which was elicited only from these areas: suppression of spontaneous activity which was typically elicited from several areas having ascending axons. One-half of these responses were accompanied by a short latency-single spike synaptic excitation. Stimulating areas more than 1.0 mm from the ascending trajectory never  produced this response, whereas the number of responses was directly related to the number of projecting axons identified in any one experiment from a given site. Thus, the predominant effect of stimulating within the ascending axonal trajectory was suppression of spontaneous activity in the projecting units, not an 'en cascade' activation of these units; on the contrary, the only type of excitation encountered was a single, short latency spike. Therefore, any effects of stimulation within the ascending reticular pathway which appear to outlast the stimulus (as previously described in the literature) cannot be ascribed to a reverberating (excitatory) circuit among projecting units. A possible source of the synaptic responses of projecting units is a retrograde activation of collaterals interconnecting the reticular cells. If such interaction exists, it is specifically distributed among cells with ascending axons, as the responses were only observed in a very few units not identified by antidromic excitation; however, other evidence is adduced to support the belief that these few units were projecting units whose axons were beyond the reach of the stimulating electrodes. Futhermore, the axons may be bundled such that units with axons nearest that of a given projecting unit give rise to the most extensive synaptic interactions; the activation of these nearby axons suppresses spontaneous activity, while axons farther away have a greater possibility of being excitatory in nature. Should such a medium for interaction exist, reticular collateral interactions might be seen to exist specifically for the purpose of decreasing the activity of cells destined for similar rostral target structures.", "contents": "Brain stem reticular units: synaptic responses to stimulation within the ascending reticular pathways. When the ascending reticular axonal system is stimulated, the responses of distal structures (e.g., the cerebral cortex) appear to outlast the stimulus; these longlasting effects could reflect the intrinsic nature of the distal structure, or the response could reflect an interaction among the reticular cells which tends to prolong the effects of stimulation. To examine the latter hypothesis, single units with ascending axons (projecting units) were recorded in the cat rostral rhombencephalon in acute experiments conducted under halothane-nitrous oxide anesthesia. Stimulation of areas to or through which axons of reticular neurons projected (midbrain tegmentum and lower tectum, medial thalamus, and basal forebrain) produced a consistent and specific response which was elicited only from these areas: suppression of spontaneous activity which was typically elicited from several areas having ascending axons. One-half of these responses were accompanied by a short latency-single spike synaptic excitation. Stimulating areas more than 1.0 mm from the ascending trajectory never  produced this response, whereas the number of responses was directly related to the number of projecting axons identified in any one experiment from a given site. Thus, the predominant effect of stimulating within the ascending axonal trajectory was suppression of spontaneous activity in the projecting units, not an 'en cascade' activation of these units; on the contrary, the only type of excitation encountered was a single, short latency spike. Therefore, any effects of stimulation within the ascending reticular pathway which appear to outlast the stimulus (as previously described in the literature) cannot be ascribed to a reverberating (excitatory) circuit among projecting units. A possible source of the synaptic responses of projecting units is a retrograde activation of collaterals interconnecting the reticular cells. If such interaction exists, it is specifically distributed among cells with ascending axons, as the responses were only observed in a very few units not identified by antidromic excitation; however, other evidence is adduced to support the belief that these few units were projecting units whose axons were beyond the reach of the stimulating electrodes. Futhermore, the axons may be bundled such that units with axons nearest that of a given projecting unit give rise to the most extensive synaptic interactions; the activation of these nearby axons suppresses spontaneous activity, while axons farther away have a greater possibility of being excitatory in nature. Should such a medium for interaction exist, reticular collateral interactions might be seen to exist specifically for the purpose of decreasing the activity of cells destined for similar rostral target structures.", "PMID": 953709}
{"id": "pubmed23n0032_700", "title": "Intracellular study of synaptic events related to phase-locking  responses of cat cochlear nucleus cells to low frequency tones.", "content": "In this study intracellular recording techniques were used to study the synaptic events related to phase-locking of cochlear nucleus cells to low frequency stimuli. A variable degree of phase-locking was noted even with units of the same low characteristic frequency. With low frequency phase-locking units an excitatory postsynaptic potential (EPSP) occurred in response to each period of the frequency stimulus, but the probability of an action potential occurring decreased as the frequency of the stimulus was raised. Complex units were described which phase-locked at lower frequencies of stimulation but did not at higher frequencies where the temporal pattern of firing to tone burst stimulation changed as well. Results are discussed as they relate to  the frequency following response recorded with gross electrodes in the lower auditory pathway and the relationship to frequency coding in the auditory pathway.", "contents": "Intracellular study of synaptic events related to phase-locking  responses of cat cochlear nucleus cells to low frequency tones. In this study intracellular recording techniques were used to study the synaptic events related to phase-locking of cochlear nucleus cells to low frequency stimuli. A variable degree of phase-locking was noted even with units of the same low characteristic frequency. With low frequency phase-locking units an excitatory postsynaptic potential (EPSP) occurred in response to each period of the frequency stimulus, but the probability of an action potential occurring decreased as the frequency of the stimulus was raised. Complex units were described which phase-locked at lower frequencies of stimulation but did not at higher frequencies where the temporal pattern of firing to tone burst stimulation changed as well. Results are discussed as they relate to  the frequency following response recorded with gross electrodes in the lower auditory pathway and the relationship to frequency coding in the auditory pathway.", "PMID": 953710}
{"id": "pubmed23n0032_701", "title": "Immunoelectrophoretic determination of brain-specific antigens in bulk-prepared neuronal and glial cells.", "content": "The relative amounts of brain-specific antigens, S-100, glial fibrillary acidic protein (GFA), 14.3.2, synaptin C1, D1, D2 and D3 were determined by crossed immunoelectrophoresis in bulk-prepared neuronal, glial and synaptosomal fractions from 35-day-old rat brains. Little enrichment was ontained for any antigen in the neuronal perikaryal fraction. The glial fraction showed a 3.5-fold enrichment in GFA but had levels similar to whole brain with respect to S-100. Synaptosomes were somewhat enriched in the synaptin C1 protein, but did not differ markedly from whole brain with respect to the most antigens. The extent  of cross-contamination in the various fractions could be judged only tentatively, partly due to differences in extractability of the proteins.", "contents": "Immunoelectrophoretic determination of brain-specific antigens in bulk-prepared neuronal and glial cells. The relative amounts of brain-specific antigens, S-100, glial fibrillary acidic protein (GFA), 14.3.2, synaptin C1, D1, D2 and D3 were determined by crossed immunoelectrophoresis in bulk-prepared neuronal, glial and synaptosomal fractions from 35-day-old rat brains. Little enrichment was ontained for any antigen in the neuronal perikaryal fraction. The glial fraction showed a 3.5-fold enrichment in GFA but had levels similar to whole brain with respect to S-100. Synaptosomes were somewhat enriched in the synaptin C1 protein, but did not differ markedly from whole brain with respect to the most antigens. The extent  of cross-contamination in the various fractions could be judged only tentatively, partly due to differences in extractability of the proteins.", "PMID": 953711}
{"id": "pubmed23n0032_702", "title": "The effect of trauma on the activity of central noradrenergic neurones.", "content": "Changes in the concentration of the main noradrenaline metabolite in rat brain, 4-hydroxy-3-methoxy-phenylethyleneglycol sulphate (MOPEG-SO4) have been studied during and after injury by limb ischaemia. In the hypothalamus the MOPEG-SO4 concentration rose in about 30 min to a new plateau level which was maintained during a 4 h period of bilateral hind-limb ischaemia and then rose further when the circulation to the limbs was restored. After about 2 h hind-limb ischaemia the concentration in the hind-brain, particularly in the caudal half, rose progressively and this rise continued when the tourniquets were removed after 4 h. Limb ischaemia did not affect the MOPEG-SO4 concentration in the cerebellum but the concentration rose in the cerebrospinal fluid. Attempts to study the production of MOPEG-SO4 in more detail by inhibiting its transport from the brain with probenecid were not helpful and reasons are given for thinking that probenecid is not a useful tool for this type of  investigation. The results are considered to provide further evidence that trauma activates ascending and descending pathways which arise from the noradrenergic nerve cells in the hind-brain.", "contents": "The effect of trauma on the activity of central noradrenergic neurones. Changes in the concentration of the main noradrenaline metabolite in rat brain, 4-hydroxy-3-methoxy-phenylethyleneglycol sulphate (MOPEG-SO4) have been studied during and after injury by limb ischaemia. In the hypothalamus the MOPEG-SO4 concentration rose in about 30 min to a new plateau level which was maintained during a 4 h period of bilateral hind-limb ischaemia and then rose further when the circulation to the limbs was restored. After about 2 h hind-limb ischaemia the concentration in the hind-brain, particularly in the caudal half, rose progressively and this rise continued when the tourniquets were removed after 4 h. Limb ischaemia did not affect the MOPEG-SO4 concentration in the cerebellum but the concentration rose in the cerebrospinal fluid. Attempts to study the production of MOPEG-SO4 in more detail by inhibiting its transport from the brain with probenecid were not helpful and reasons are given for thinking that probenecid is not a useful tool for this type of  investigation. The results are considered to provide further evidence that trauma activates ascending and descending pathways which arise from the noradrenergic nerve cells in the hind-brain.", "PMID": 953712}
{"id": "pubmed23n0032_703", "title": "Release of exqenous glycine in the pigeon optic tectum during stimulation of a midbrain nucleus.", "content": "A pathway having an affinity for glycine has been investigated in the pigeon optic lobe; it originates in the nucleus isthmi pars parvocellularis (Ipc) and terminates in the tectum, In an attempt to obtain evidence that glycine plays a role as a transmitter in this system, the effect of electrical stimulation on release of labeled substances previously injected in the tectum was tested. By perfusing the upper strata of the optic tectum with a push-pull cannula the release of radioactive glycine was shown to be markedly increased by electrical stimulation of Ipc, but not by stimulation of other sites. Ipc stimulation did not affect the efflux of exogenous leucine or urea, whereas a GABA release was observed. With K+ (40 mM) stimulation all amino acids tested were released. It is suggested that the Ipc neuron terminals in the tectum take up glycine and release it upon stimulation of the Ipc nucleus.", "contents": "Release of exqenous glycine in the pigeon optic tectum during stimulation of a midbrain nucleus. A pathway having an affinity for glycine has been investigated in the pigeon optic lobe; it originates in the nucleus isthmi pars parvocellularis (Ipc) and terminates in the tectum, In an attempt to obtain evidence that glycine plays a role as a transmitter in this system, the effect of electrical stimulation on release of labeled substances previously injected in the tectum was tested. By perfusing the upper strata of the optic tectum with a push-pull cannula the release of radioactive glycine was shown to be markedly increased by electrical stimulation of Ipc, but not by stimulation of other sites. Ipc stimulation did not affect the efflux of exogenous leucine or urea, whereas a GABA release was observed. With K+ (40 mM) stimulation all amino acids tested were released. It is suggested that the Ipc neuron terminals in the tectum take up glycine and release it upon stimulation of the Ipc nucleus.", "PMID": 953713}
{"id": "pubmed23n0032_704", "title": "Developmental changes in brain glycoproteins.", "content": "During the postnatal development of rat brain there are large increases in the concentration of brain glycoproteins. Between 1 and 30 days the greatest changes (70-100%) take place in the levels of glycoprotein mannose, galactose and glucosamine, accompanied by smaller increases (35-55%) in sialic acid and fucose. By 30 days of age levels of brain glycoproteins are within 5% of the adult values. Analyses of the molecular size and composition of glycopeptides prepared from brains of 1- and 30-day-old rats lead to the conclusion that during postnatal brain development there is a preferential synthesis of a distinct population of glycoproteins containing oligosaccharides consisting predominantly of glucosamine, mannose and galactose. These oligosaccharides therefore have a large 'core' segment and a relative deficiency of the characteristically terminal sugars, fucose and sialic acid. In very young rat brain there are also large amounts of a metabolically stable form of glycogen or limit dextrin which accompanies the glycopeptides through the usual methods involved in their preparation from brain glycoproteins. The concentration of this glucose polymer decreases by 93% within 30 days after birth, but its presence even in adult brain is a likely explanation for the numerous reports of small amounts of glucose in brain glycopeptides and glycoproteins.", "contents": "Developmental changes in brain glycoproteins. During the postnatal development of rat brain there are large increases in the concentration of brain glycoproteins. Between 1 and 30 days the greatest changes (70-100%) take place in the levels of glycoprotein mannose, galactose and glucosamine, accompanied by smaller increases (35-55%) in sialic acid and fucose. By 30 days of age levels of brain glycoproteins are within 5% of the adult values. Analyses of the molecular size and composition of glycopeptides prepared from brains of 1- and 30-day-old rats lead to the conclusion that during postnatal brain development there is a preferential synthesis of a distinct population of glycoproteins containing oligosaccharides consisting predominantly of glucosamine, mannose and galactose. These oligosaccharides therefore have a large 'core' segment and a relative deficiency of the characteristically terminal sugars, fucose and sialic acid. In very young rat brain there are also large amounts of a metabolically stable form of glycogen or limit dextrin which accompanies the glycopeptides through the usual methods involved in their preparation from brain glycoproteins. The concentration of this glucose polymer decreases by 93% within 30 days after birth, but its presence even in adult brain is a likely explanation for the numerous reports of small amounts of glucose in brain glycopeptides and glycoproteins.", "PMID": 953714}
{"id": "pubmed23n0032_705", "title": "Laminar patterns of geniculocortical projection in the cat.", "content": "The cortical afferents from individual laminae of the dorsal lateral geniculate nucleus (LGN) were studied using both light and electron microscope autoradiography. In area 17, the A geniculate laminae (A and A1) had two main bands of projection, one extending from the bottom of IVc to the deepest cells in layer III, and one in layer VI. The C geniculate laminae projected in two dense bands to the upper and lower borders of layer IV, thus bracketing the A laminae projection, though with some overlap. In addition, the C laminae projected to the superficial half of layer I, which the A laminae did not. Conversely, while the A laminae projected to layer VI, the C laminae did not. The two sets of laminae also showed differences in the areas to which they projected. The A geniculate laminae projected to areas 17 and 18, whereas the C geniculate laminae had a more extensive projection, including areas 17, 18, 19 and other areas on the suprasylvian gyrus. The laminar organization of the projection to area 18 was similar to that found in area 17. At the electron microscopic level the geniculate terminals were found to make Gray's type 1 synapses, for the most part onto dendritic spines. Labeled terminals were found in all the projection bands seen in the light microscope. The implications of these findings on the connectivity of cells in layer IV are discussed. The presence of labeled terminals in layer VI, which contains the cells of origin of the corticogeniculate pathway, suggests that the recurrent loop to the LGN is mediated monosynaptically. Finally, the afferents from each geniculate lamina were found to be segregated into patches, about 500 mum wide, which probably form the anatomical basis for ocular dominance columns.", "contents": "Laminar patterns of geniculocortical projection in the cat. The cortical afferents from individual laminae of the dorsal lateral geniculate nucleus (LGN) were studied using both light and electron microscope autoradiography. In area 17, the A geniculate laminae (A and A1) had two main bands of projection, one extending from the bottom of IVc to the deepest cells in layer III, and one in layer VI. The C geniculate laminae projected in two dense bands to the upper and lower borders of layer IV, thus bracketing the A laminae projection, though with some overlap. In addition, the C laminae projected to the superficial half of layer I, which the A laminae did not. Conversely, while the A laminae projected to layer VI, the C laminae did not. The two sets of laminae also showed differences in the areas to which they projected. The A geniculate laminae projected to areas 17 and 18, whereas the C geniculate laminae had a more extensive projection, including areas 17, 18, 19 and other areas on the suprasylvian gyrus. The laminar organization of the projection to area 18 was similar to that found in area 17. At the electron microscopic level the geniculate terminals were found to make Gray's type 1 synapses, for the most part onto dendritic spines. Labeled terminals were found in all the projection bands seen in the light microscope. The implications of these findings on the connectivity of cells in layer IV are discussed. The presence of labeled terminals in layer VI, which contains the cells of origin of the corticogeniculate pathway, suggests that the recurrent loop to the LGN is mediated monosynaptically. Finally, the afferents from each geniculate lamina were found to be segregated into patches, about 500 mum wide, which probably form the anatomical basis for ocular dominance columns.", "PMID": 953720}
{"id": "pubmed23n0032_706", "title": "The binding properties and regional ontogeny of receptors for alpha-bungarotoxin in chick brain.", "content": "The subcellular distribution, binding kinetics, pharmacologic properties and regional ontogeny of binding sites for alpha-[125I]bungarotoxins (alpha-[125I]BT) were studied in preparations of chick brain. Almost 95% of the specific binding was found in a 20,000 X g pellet. Two binding components were found, one with high (half-saturation at 1.5 X 10(-9) M) and another with low affinity for the toxin. The rate constants of association and dissociation of the toxin for the high-affinity site were found to be 3.5 X 10(4) M-1 - sec-1 and 3.8 X 10(-5) sec-1 (tau1/2 = 5.1 h, respectively). These data yielded a K alpha value of 1.1 X 10(-9) M. Nicotinic, but not muscarinic ligands were potent inhibitors of toxin binding at the high-affinity site. Thus, as in muscle, the binding site for toxin in chick brain appears to have the properties of a nicotinic acetylcholine (ACh) receptor. The ontogeny of the alpha-BT/ACh receptor was studied using homogenates of both whole brain and of discrete brain regions. In brain stem, optic lobe and cerebellum specific binding reached maximum levels in ovo and then decreased by maturity to values of 10,22 and 4.5 fmoles/mg protein, respectively. In contrast, whole brain and cerebral hemispheres reached plateau levels in ovo of 17 and 10 fmoles/mg protein, respectively. Major increases in total receptor number per region appeared in ovo between days 12 and 19 in brain stem and cerebellum, days 12 and 15 in optic lobes, and days 15 and 19 in hemispheres. The hemispheres were the only region to show a continued increase in total binding after hatching (3-fold increase by maturity). These findings are interpreted with respect to the development of chick brain and a model is suggested for the relationship between receptor ontogeny and neuronal differentiation and maturation.", "contents": "The binding properties and regional ontogeny of receptors for alpha-bungarotoxin in chick brain. The subcellular distribution, binding kinetics, pharmacologic properties and regional ontogeny of binding sites for alpha-[125I]bungarotoxins (alpha-[125I]BT) were studied in preparations of chick brain. Almost 95% of the specific binding was found in a 20,000 X g pellet. Two binding components were found, one with high (half-saturation at 1.5 X 10(-9) M) and another with low affinity for the toxin. The rate constants of association and dissociation of the toxin for the high-affinity site were found to be 3.5 X 10(4) M-1 - sec-1 and 3.8 X 10(-5) sec-1 (tau1/2 = 5.1 h, respectively). These data yielded a K alpha value of 1.1 X 10(-9) M. Nicotinic, but not muscarinic ligands were potent inhibitors of toxin binding at the high-affinity site. Thus, as in muscle, the binding site for toxin in chick brain appears to have the properties of a nicotinic acetylcholine (ACh) receptor. The ontogeny of the alpha-BT/ACh receptor was studied using homogenates of both whole brain and of discrete brain regions. In brain stem, optic lobe and cerebellum specific binding reached maximum levels in ovo and then decreased by maturity to values of 10,22 and 4.5 fmoles/mg protein, respectively. In contrast, whole brain and cerebral hemispheres reached plateau levels in ovo of 17 and 10 fmoles/mg protein, respectively. Major increases in total receptor number per region appeared in ovo between days 12 and 19 in brain stem and cerebellum, days 12 and 15 in optic lobes, and days 15 and 19 in hemispheres. The hemispheres were the only region to show a continued increase in total binding after hatching (3-fold increase by maturity). These findings are interpreted with respect to the development of chick brain and a model is suggested for the relationship between receptor ontogeny and neuronal differentiation and maturation.", "PMID": 953721}
{"id": "pubmed23n0032_707", "title": "Action of amino acids and convulsants on cerebellar spontaneous action potentials in vitro: effects of deprivation of C1-, K+ of Na+.", "content": "(1) The inhibition of spontaneous action potentials in guinea pig cerebellar cortex slices by GABA, glycine, taurine and beta-alanine is maintained when C1- in the superfusion medium is almost completely replaced by NO3- or I-('permeant' anion), but the inhibition decreases in magnitude with repeated application of the amino acid. Replacement of C1- by sulfate or isethionate ('impermeant' anion) causes a conversion of inhibition by these amino acids to excitation. The initial excitation which is sometimes seen with these inhibitory amino acids in high C1- media is abolished when C1- is replaced by either permeant or impermeant anions. (2) Reduction of K+ in the medium causes an increase of inhibition by the inhibitory amino acids in the presence of high C1- and reduction of excitation when C1- is replaced by impermeant anion. (3) Excitation by GABA in impermeant anion (low C1-) media  is unaffected by reduction of Na+ in the media by 50% but excitations by glycine, taurine, beta-alanine and L-glutamate are greatly reduced. (4). Excitation by GABA in impermeant anion (low C1-) media is abolished by picrotoxin and bicuculline which both suppress inhibition by GABA in a high C1- medium. Strychnine suppresses the effects of glycine, taurine and beta-alanine in either a low or high C1- medium. Bicuculline blocks the inhibitory effect of these three amino acids in a high C1- medium but does not affect their excitatory effects in a low C1- medium. (5) These results are consistent with the hypothesis that the inhibitory amino acids, GABA, glycine, taurine and beta-alanine, cause inhibition via increase of C1- (and perhaps K+) permeability and that glycine, taurine and beta-alanine also interact with strychnine-sensitive receptors mediating (perhaps indirectly) increased permeability to Na+ and, therefore, excitation in low C1- media.", "contents": "Action of amino acids and convulsants on cerebellar spontaneous action potentials in vitro: effects of deprivation of C1-, K+ of Na+. (1) The inhibition of spontaneous action potentials in guinea pig cerebellar cortex slices by GABA, glycine, taurine and beta-alanine is maintained when C1- in the superfusion medium is almost completely replaced by NO3- or I-('permeant' anion), but the inhibition decreases in magnitude with repeated application of the amino acid. Replacement of C1- by sulfate or isethionate ('impermeant' anion) causes a conversion of inhibition by these amino acids to excitation. The initial excitation which is sometimes seen with these inhibitory amino acids in high C1- media is abolished when C1- is replaced by either permeant or impermeant anions. (2) Reduction of K+ in the medium causes an increase of inhibition by the inhibitory amino acids in the presence of high C1- and reduction of excitation when C1- is replaced by impermeant anion. (3) Excitation by GABA in impermeant anion (low C1-) media  is unaffected by reduction of Na+ in the media by 50% but excitations by glycine, taurine, beta-alanine and L-glutamate are greatly reduced. (4). Excitation by GABA in impermeant anion (low C1-) media is abolished by picrotoxin and bicuculline which both suppress inhibition by GABA in a high C1- medium. Strychnine suppresses the effects of glycine, taurine and beta-alanine in either a low or high C1- medium. Bicuculline blocks the inhibitory effect of these three amino acids in a high C1- medium but does not affect their excitatory effects in a low C1- medium. (5) These results are consistent with the hypothesis that the inhibitory amino acids, GABA, glycine, taurine and beta-alanine, cause inhibition via increase of C1- (and perhaps K+) permeability and that glycine, taurine and beta-alanine also interact with strychnine-sensitive receptors mediating (perhaps indirectly) increased permeability to Na+ and, therefore, excitation in low C1- media.", "PMID": 953722}
{"id": "pubmed23n0032_708", "title": "Effects of reciprocal forebrain transplantation on motility and hatching in chick and duck embryos.", "content": "Effects of reciprocal forebrain transplantation on the embryonic motility and the hatching behavior in the chick and the duck embryos were studied. The forebrains were transplanted before the establishment of circulation. The grafted tissue formed the telencephalon, diencephalon, eyes, upper beak and part of the cranium. Data indicate that the size of the forebrain has no significant influence on the early embryonic motility (Type I and II) in the chick and the duck embryos. The initiation and maintenance of the pre-hatching behavior (Type III motility) does not reside in the forebrain. The final stage of hatching (climax) on the other hand may be controlled by the forebrain.", "contents": "Effects of reciprocal forebrain transplantation on motility and hatching in chick and duck embryos. Effects of reciprocal forebrain transplantation on the embryonic motility and the hatching behavior in the chick and the duck embryos were studied. The forebrains were transplanted before the establishment of circulation. The grafted tissue formed the telencephalon, diencephalon, eyes, upper beak and part of the cranium. Data indicate that the size of the forebrain has no significant influence on the early embryonic motility (Type I and II) in the chick and the duck embryos. The initiation and maintenance of the pre-hatching behavior (Type III motility) does not reside in the forebrain. The final stage of hatching (climax) on the other hand may be controlled by the forebrain.", "PMID": 953732}
{"id": "pubmed23n0032_709", "title": "Evidence for the existence of a raphe projection to the substantia nigra in rat.", "content": "The effect of electrical stimulation of the median raphe nucleus on the activity of spontaneously firing single neurones in the substantia nigra and mesencephalic reticular formation (MRF) has been investigated in urethane anaesthetized rats. Depression of activity was the predominant effect observed although this was sometimes accompanied by periods of excitation. Some neurones were only excited. The latency of inhibition of substantia nigra neurones was constant whereas that of MRF neurones was more variable. Microiontophoretically applied 5-hydroxy-tryptamine (5-HT) and dopamine (DA) produced mainly inhibition of neuronal activity, but excitation and biphasic effects were also seen. There was a good correlation between the direction of neuronal responses in the substantia nigra to median raphe stimulation and to the effects of 5-HT but not DA. Discrete electrolytic lesions of the median raphe nucleus were followed by a decrease in 5-HT but not GABA concentrations in the substantia nigra. In addition striatal 5-HT, GABA and NA concentrations were unchanged whereas striatal DA was increased. These observations strongly suggest that the substantia nigra receives a direct inhibitory input from the median raphe nucleus and this pathway uses a 5-HT-like neurotransmitter. This pathway probably contributes to the regulation of nigrostriatal dopaminergic transmission.", "contents": "Evidence for the existence of a raphe projection to the substantia nigra in rat. The effect of electrical stimulation of the median raphe nucleus on the activity of spontaneously firing single neurones in the substantia nigra and mesencephalic reticular formation (MRF) has been investigated in urethane anaesthetized rats. Depression of activity was the predominant effect observed although this was sometimes accompanied by periods of excitation. Some neurones were only excited. The latency of inhibition of substantia nigra neurones was constant whereas that of MRF neurones was more variable. Microiontophoretically applied 5-hydroxy-tryptamine (5-HT) and dopamine (DA) produced mainly inhibition of neuronal activity, but excitation and biphasic effects were also seen. There was a good correlation between the direction of neuronal responses in the substantia nigra to median raphe stimulation and to the effects of 5-HT but not DA. Discrete electrolytic lesions of the median raphe nucleus were followed by a decrease in 5-HT but not GABA concentrations in the substantia nigra. In addition striatal 5-HT, GABA and NA concentrations were unchanged whereas striatal DA was increased. These observations strongly suggest that the substantia nigra receives a direct inhibitory input from the median raphe nucleus and this pathway uses a 5-HT-like neurotransmitter. This pathway probably contributes to the regulation of nigrostriatal dopaminergic transmission.", "PMID": 953733}
{"id": "pubmed23n0032_710", "title": "Attenuation of self-stimulation from substantia nigra but not dorsal tegmental noradrenergic bundle by lesions of sulcal prefrontal cortex.", "content": "Rats that self-stimulated from electrodes implanted in either the substantia nigra pars compacta (SNC) or the dorsal tegmental noradrenergic bundle (DTB) received bilateral electrolytic lesions of the prefrontal cortex dorsal to the rhinal sulcus. Immediately after the lesions, animals with SNC electrodes (n = 9) showed significant and permanent reductions in self-stimulation rates. The average reduction in the SNC group was 67% of prelesion bar-pressing scores. In contrast, rats with DTB electrodes (n = 11) were not significantly affected by similar sulcal lesions. Subsequent examination of the brains for prograde degeneration using procedure I of Fink and Heimer 11 revealed a descending system of sulcal efferents that was very dense in the region of the SNC but only scattered in the area of the DTB. The close relation between the effects of sulcal lesions on self-stimulation and the resultant density of degeneration as a function of stimulation site is discussed in terms of the contribution of prefrontal cortex to self-stimulation in general, and of the implications for the catecholamine hypothesis of self-stimulation in particular.", "contents": "Attenuation of self-stimulation from substantia nigra but not dorsal tegmental noradrenergic bundle by lesions of sulcal prefrontal cortex. Rats that self-stimulated from electrodes implanted in either the substantia nigra pars compacta (SNC) or the dorsal tegmental noradrenergic bundle (DTB) received bilateral electrolytic lesions of the prefrontal cortex dorsal to the rhinal sulcus. Immediately after the lesions, animals with SNC electrodes (n = 9) showed significant and permanent reductions in self-stimulation rates. The average reduction in the SNC group was 67% of prelesion bar-pressing scores. In contrast, rats with DTB electrodes (n = 11) were not significantly affected by similar sulcal lesions. Subsequent examination of the brains for prograde degeneration using procedure I of Fink and Heimer 11 revealed a descending system of sulcal efferents that was very dense in the region of the SNC but only scattered in the area of the DTB. The close relation between the effects of sulcal lesions on self-stimulation and the resultant density of degeneration as a function of stimulation site is discussed in terms of the contribution of prefrontal cortex to self-stimulation in general, and of the implications for the catecholamine hypothesis of self-stimulation in particular.", "PMID": 953734}
{"id": "pubmed23n0032_711", "title": "Evidence that self-stimulation of the region of the locus coeruleus in rats does not depend upon noradrenergic projections to telencephalon.", "content": "Rats with intracranial self-stimulation (ICSS) electrodes in the locus coeruleus and adjacent pontine tegmental structures received stereotaxically placed bilateral injections of 6-hydroxydopamine (4 mug/2 mul) into the mesencephalic trajectory of the dorsal tegmental noradrenergic bundle. The consequent depletions of norepinephrine in the cerebral cortices and hippocampi (96.7%) did not result in significant changes in ICSS. Thus, diencephalic and telencephalic noradrenergic projections of the locus coeruleus do not appear to be critical for the occurrence of ICSS from that nucleus or its surrounding region. Nor do these projections appear to be crucially involved in the enhancement of this ICSS by D-amphetamine. Rats in this study showed two-fold increases in responding following injections of D-amphetamine sulfate (0.5 mg/kg) both before and after the lesions of the dorsal tegmental bundle. These results suggest that the ascending projections of the locus coeruleus are not critically involved in ICSS of the dorsal pontine tegmentum.", "contents": "Evidence that self-stimulation of the region of the locus coeruleus in rats does not depend upon noradrenergic projections to telencephalon. Rats with intracranial self-stimulation (ICSS) electrodes in the locus coeruleus and adjacent pontine tegmental structures received stereotaxically placed bilateral injections of 6-hydroxydopamine (4 mug/2 mul) into the mesencephalic trajectory of the dorsal tegmental noradrenergic bundle. The consequent depletions of norepinephrine in the cerebral cortices and hippocampi (96.7%) did not result in significant changes in ICSS. Thus, diencephalic and telencephalic noradrenergic projections of the locus coeruleus do not appear to be critical for the occurrence of ICSS from that nucleus or its surrounding region. Nor do these projections appear to be crucially involved in the enhancement of this ICSS by D-amphetamine. Rats in this study showed two-fold increases in responding following injections of D-amphetamine sulfate (0.5 mg/kg) both before and after the lesions of the dorsal tegmental bundle. These results suggest that the ascending projections of the locus coeruleus are not critically involved in ICSS of the dorsal pontine tegmentum.", "PMID": 953735}
{"id": "pubmed23n0032_712", "title": "Sensory and reflex responses to tooth pulp stimulation in man.", "content": "Experiments have been carried out to investigate whether all tooth pulp afferent nerves are capable of producing pain. Monopolar and bipolar stimuli were applied to teeth in human subjects and sensory thresholds determined. EMGs were recorded from the masseter and the anterior digastric muscles. With stimuli up to three times the sensory threshold, no response could be detected in the digastric but at, or just above, the sensory threshold, inhibitory effects were produced in masseter muscle. The latency of the muscle response with bipolar stimulation was 18-22 msec. There was no evidence of stimulus spread to nerves outside the teeth. Bipolar and monopolar stimulation both produced the same sensation but this was not described as painful. It is concluded that some pulpal afferent nerves may not be capable of producing pain, and that the sensory and reflex responses at threshold were probably produced by the same fibres.", "contents": "Sensory and reflex responses to tooth pulp stimulation in man. Experiments have been carried out to investigate whether all tooth pulp afferent nerves are capable of producing pain. Monopolar and bipolar stimuli were applied to teeth in human subjects and sensory thresholds determined. EMGs were recorded from the masseter and the anterior digastric muscles. With stimuli up to three times the sensory threshold, no response could be detected in the digastric but at, or just above, the sensory threshold, inhibitory effects were produced in masseter muscle. The latency of the muscle response with bipolar stimulation was 18-22 msec. There was no evidence of stimulus spread to nerves outside the teeth. Bipolar and monopolar stimulation both produced the same sensation but this was not described as painful. It is concluded that some pulpal afferent nerves may not be capable of producing pain, and that the sensory and reflex responses at threshold were probably produced by the same fibres.", "PMID": 953736}
{"id": "pubmed23n0032_713", "title": "Neurite outgrowth from explanted Xenopus retina:  an effect of prior optic nerve section.", "content": "Experimental conditions are described for the in vitro study of explanted eyes from embryos of Xenopus laevis and of retinas from older larvae. When eyes are explanted from embryos of stages 25-34, a rapid outgrowth of fibroblast-like and pigment cells is observed, upon which a neuritic outgrowth is eventually superimposed. Outgrowth from the retina of later stage tadpoles (50-54) is not seen until about a week following explantation and resembles the mixed cell outgrowth observed in whole eye explants from early stage embryos. If, however, the optic nerve of an older tadpole is cut 7 days prior to explantation, a purely neuritic outgrowth is seen from the previously denervated retina  within 1-3 days.", "contents": "Neurite outgrowth from explanted Xenopus retina:  an effect of prior optic nerve section. Experimental conditions are described for the in vitro study of explanted eyes from embryos of Xenopus laevis and of retinas from older larvae. When eyes are explanted from embryos of stages 25-34, a rapid outgrowth of fibroblast-like and pigment cells is observed, upon which a neuritic outgrowth is eventually superimposed. Outgrowth from the retina of later stage tadpoles (50-54) is not seen until about a week following explantation and resembles the mixed cell outgrowth observed in whole eye explants from early stage embryos. If, however, the optic nerve of an older tadpole is cut 7 days prior to explantation, a purely neuritic outgrowth is seen from the previously denervated retina  within 1-3 days.", "PMID": 953737}
{"id": "pubmed23n0032_714", "title": "Axon regeneration following a lesion of the carotid nerve: electrophysiological and ultrastructural observations.", "content": "Carotid nerves of the cat were crushed and allowed to regenerate in order to study the properties of regerating fibers and the role of carotid body parenchymal cells (glomus or type I, and sustentacular or type II) in the transduction of chemosensory activity. Such activity is reinitiated 6 days after the nerves are crushed close (1-2 mm) to the carotid body. The process of recovery is delayed when a crush is made at successively greater distances (5-6 and 10-12 mm) from the carotid body. Ultrastructural studies show that the  reappearance of nerve endings on the glomus-sustentacular cell complex coincides in time with the onset of chemosensory activity. The  regenerated nerve endings increase in size and number and appear normal by 48 days. Some barosensory activity can be elicited 6 days after a nerve crush close to the carotid sinus, but rhythmic barosensory discharges  only occur after the 21st  day when myelinated axons  reappear in the carotid sinus adventitia. Results suggest that recovery of chemosensory function depends on the reestablishment of apposition between regenerating carotid nerve fibers and parenchymal cells of the carotid body.", "contents": "Axon regeneration following a lesion of the carotid nerve: electrophysiological and ultrastructural observations. Carotid nerves of the cat were crushed and allowed to regenerate in order to study the properties of regerating fibers and the role of carotid body parenchymal cells (glomus or type I, and sustentacular or type II) in the transduction of chemosensory activity. Such activity is reinitiated 6 days after the nerves are crushed close (1-2 mm) to the carotid body. The process of recovery is delayed when a crush is made at successively greater distances (5-6 and 10-12 mm) from the carotid body. Ultrastructural studies show that the  reappearance of nerve endings on the glomus-sustentacular cell complex coincides in time with the onset of chemosensory activity. The  regenerated nerve endings increase in size and number and appear normal by 48 days. Some barosensory activity can be elicited 6 days after a nerve crush close to the carotid sinus, but rhythmic barosensory discharges  only occur after the 21st  day when myelinated axons  reappear in the carotid sinus adventitia. Results suggest that recovery of chemosensory function depends on the reestablishment of apposition between regenerating carotid nerve fibers and parenchymal cells of the carotid body.", "PMID": 953738}
{"id": "pubmed23n0032_715", "title": "Visual cortical neurons influenced by the oculomotor input: characterization of their receptive field properties.", "content": "A class of cells in cat visual cortex (area 17 and 18) had a tight correlation with spontaneous and electrically evoked PGO waves under reserpine. They tended to have high maintained activity and a large receptive field which was preferentially excited by fast moving slits. They were also direction selective and influenced through both eyes. For these cells, the selectivity for visual stimuli was dramatically altered in the presence of PGO waves induced by pontine stimulation. They were complex cells. A second class of cortical cells showed a moderate correlation with spontaneous PGO waves. Visually evoked activity of these cells was either excited or inhibited  by evoked PGO waves in response to pontine stimulation. A third class of cells, the majority, did not seem to have any correlation with PGO waves. The second and third classes of cells could be either one of the 3 main categories of visual cortical cells, predominantly simple cells in area 17 and complex cells in area 18. The present study provided further support to a previous proposal that PGO waves in the visual cortex, as neural correlates of saccadic eye movements, modulate specifically the ongoing activity of a selective type of cortical cell. A type of complex cell in the deeper layers is supposed to integrate visual and oculomotor inputs. It is hypothesized that the consequence of oculomotor inputs to the visual cortex is specified by the type of cells which receive oculomotor inputs, rather than by the nature of the inputs themselves.", "contents": "Visual cortical neurons influenced by the oculomotor input: characterization of their receptive field properties. A class of cells in cat visual cortex (area 17 and 18) had a tight correlation with spontaneous and electrically evoked PGO waves under reserpine. They tended to have high maintained activity and a large receptive field which was preferentially excited by fast moving slits. They were also direction selective and influenced through both eyes. For these cells, the selectivity for visual stimuli was dramatically altered in the presence of PGO waves induced by pontine stimulation. They were complex cells. A second class of cortical cells showed a moderate correlation with spontaneous PGO waves. Visually evoked activity of these cells was either excited or inhibited  by evoked PGO waves in response to pontine stimulation. A third class of cells, the majority, did not seem to have any correlation with PGO waves. The second and third classes of cells could be either one of the 3 main categories of visual cortical cells, predominantly simple cells in area 17 and complex cells in area 18. The present study provided further support to a previous proposal that PGO waves in the visual cortex, as neural correlates of saccadic eye movements, modulate specifically the ongoing activity of a selective type of cortical cell. A type of complex cell in the deeper layers is supposed to integrate visual and oculomotor inputs. It is hypothesized that the consequence of oculomotor inputs to the visual cortex is specified by the type of cells which receive oculomotor inputs, rather than by the nature of the inputs themselves.", "PMID": 953739}
{"id": "pubmed23n0032_716", "title": "Studies on cellular toxicity of bilirubin: effect on brain glycolysis in the young rat.", "content": "(1) The levels of glycolytic intermediates in the brain tissue of  15-day-old homozygous and heterozygous Gunn rats were determined with or without novobiocin treatment in vivo, and the inhibitory effects of bilirubin on the glycolytic activity and the key glycolytic enzyme activities were investigated in vitro. (2) the pattern of glycolytic intermediate levels in the non-treated homozygotes compared with those in the non-treated heterozygotes  showed a significant increase of G6P and F6P,  and  decrease of GAP plus DAP. Enhancement of bilirubin concentration in the brain tissue of homozygotes by novobiocin treatment brought about a significant decrease of FDP, 3PG, Pyr and Lac, and a slight increase of G6P compared with the respective values on the treated heterozygotes. (3) the overall glycolytic activity with 2mM glucose as a substrate was significantly inhibited in vitro by 100 muM bilirubin at 3-4, 20 and 600muM NAD concentration, whereas with 0.15 mM glucose the inhibition was observed only at a concentration of 3-4muM NAD. The inhibitory effect of bilirubin (120-140 muM) on lactate formation with FDP as a substrate was also found at three different NAD concentrations. And the marked inhibition of PFK activity by bilirubin was observed at physiological concentrations of ATP  and F6P. (4) The present findings suggest that reduced rates of glycolysis might be involved in bilirubin encephalopathy.", "contents": "Studies on cellular toxicity of bilirubin: effect on brain glycolysis in the young rat. (1) The levels of glycolytic intermediates in the brain tissue of  15-day-old homozygous and heterozygous Gunn rats were determined with or without novobiocin treatment in vivo, and the inhibitory effects of bilirubin on the glycolytic activity and the key glycolytic enzyme activities were investigated in vitro. (2) the pattern of glycolytic intermediate levels in the non-treated homozygotes compared with those in the non-treated heterozygotes  showed a significant increase of G6P and F6P,  and  decrease of GAP plus DAP. Enhancement of bilirubin concentration in the brain tissue of homozygotes by novobiocin treatment brought about a significant decrease of FDP, 3PG, Pyr and Lac, and a slight increase of G6P compared with the respective values on the treated heterozygotes. (3) the overall glycolytic activity with 2mM glucose as a substrate was significantly inhibited in vitro by 100 muM bilirubin at 3-4, 20 and 600muM NAD concentration, whereas with 0.15 mM glucose the inhibition was observed only at a concentration of 3-4muM NAD. The inhibitory effect of bilirubin (120-140 muM) on lactate formation with FDP as a substrate was also found at three different NAD concentrations. And the marked inhibition of PFK activity by bilirubin was observed at physiological concentrations of ATP  and F6P. (4) The present findings suggest that reduced rates of glycolysis might be involved in bilirubin encephalopathy.", "PMID": 953740}
{"id": "pubmed23n0032_717", "title": "Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin production.", "content": "Tritiated thymidine has been used as a nuclear marker to trace the origin of Schwann cells, sited in the distal stump of a severed unmyelinated nerve, which are able to elaborate myelin around axons regenerating from an anastomosed proximal stump of a severed myelinated nerve. Two types of cross-anastomosis experiments were performed in young, adult rats: (1) the proximal stump of a myelinated sternohyoid nerve was labeled (5 mCi/kg body weight) selectively over a 4-day period of predetermined maximal thymidine uptake and two days later, after flushing the animal repeatedly with cold thymidine, the unmyelinated cervical sympathetic trunk was transected and its unlabeled  distal stump linked to the proximal stump of the labeled sternohyoid nerve; (2) the distal stump of an unmyelinated cervical sympathetic trunk was labeled selectively over a 5-day period of predetermined maximal uptake and two days later, after flushing with cold thymidine, the myelinated sternohyoid  nerve was severed and its unlabeled  proximal stump linked to  the labeled  distal stump of the cervical sympathetic trunk. The fate of the labeled cells in each type of anastomosis was determined 3 weeks later by autoradiography and liquid scintillation spectrometry. In the first type, a small amount of label had migrated from proximal stumps but labeled Schwann cells were not found in successfully anastomosed distal stumps. In the second type, labeled Schwann cells were seen in the cervical sympathetic trunk in association with myelinated and non-myelinated axons regenerating from the sternohyoid nerve. These data suggest that the presence or absence of myelin formation by a Schwann cell is controlled by some property of the axon with which it is associated. Putative mechanisms underlying neuronal control of myelinogenesis are discussed.", "contents": "Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin production. Tritiated thymidine has been used as a nuclear marker to trace the origin of Schwann cells, sited in the distal stump of a severed unmyelinated nerve, which are able to elaborate myelin around axons regenerating from an anastomosed proximal stump of a severed myelinated nerve. Two types of cross-anastomosis experiments were performed in young, adult rats: (1) the proximal stump of a myelinated sternohyoid nerve was labeled (5 mCi/kg body weight) selectively over a 4-day period of predetermined maximal thymidine uptake and two days later, after flushing the animal repeatedly with cold thymidine, the unmyelinated cervical sympathetic trunk was transected and its unlabeled  distal stump linked to the proximal stump of the labeled sternohyoid nerve; (2) the distal stump of an unmyelinated cervical sympathetic trunk was labeled selectively over a 5-day period of predetermined maximal uptake and two days later, after flushing with cold thymidine, the myelinated sternohyoid  nerve was severed and its unlabeled  proximal stump linked to  the labeled  distal stump of the cervical sympathetic trunk. The fate of the labeled cells in each type of anastomosis was determined 3 weeks later by autoradiography and liquid scintillation spectrometry. In the first type, a small amount of label had migrated from proximal stumps but labeled Schwann cells were not found in successfully anastomosed distal stumps. In the second type, labeled Schwann cells were seen in the cervical sympathetic trunk in association with myelinated and non-myelinated axons regenerating from the sternohyoid nerve. These data suggest that the presence or absence of myelin formation by a Schwann cell is controlled by some property of the axon with which it is associated. Putative mechanisms underlying neuronal control of myelinogenesis are discussed.", "PMID": 953741}
{"id": "pubmed23n0032_718", "title": "Morphological evidence of alteration in myelinstructure with maturation.", "content": "The periodicity of the myelin sheath in glutaraldehyde carbohydrazide (GACH) embedded tissues from three 10-day-old and two 43-day-old mice was investigated with the electron microscope. The mean repeat period and number of repeats per fiber was measured in 31-89 fibers in the trapezoid bodies, olfactory tract and sciatic nerves. The mean period of the myelin sheaths in both the peripheral and the central nervous systems of the 10-day-old mice (15.1 +/- 0.07 nm (S.E.M.) and 13.4 +/- 0.09 nm respectively) are smaller (rho less than 0.001) than those of the adult mice (15.9 +/- 0.08 nm and 14.7 +/- 0.06 nm respectively). These differences may be related to the known differences in chemical composition of immature and mature myelin.", "contents": "Morphological evidence of alteration in myelinstructure with maturation. The periodicity of the myelin sheath in glutaraldehyde carbohydrazide (GACH) embedded tissues from three 10-day-old and two 43-day-old mice was investigated with the electron microscope. The mean repeat period and number of repeats per fiber was measured in 31-89 fibers in the trapezoid bodies, olfactory tract and sciatic nerves. The mean period of the myelin sheaths in both the peripheral and the central nervous systems of the 10-day-old mice (15.1 +/- 0.07 nm (S.E.M.) and 13.4 +/- 0.09 nm respectively) are smaller (rho less than 0.001) than those of the adult mice (15.9 +/- 0.08 nm and 14.7 +/- 0.06 nm respectively). These differences may be related to the known differences in chemical composition of immature and mature myelin.", "PMID": 953750}
{"id": "pubmed23n0032_719", "title": "Effects of subfornical organ extracts of salt-water balance in the rat.", "content": "The subfornical organ (SFO) is regarded as a neurosecretory structure but no information is available on the nature or biological effects of the secretory products(s). Supernatants of water homogenates of rat SFO were lyophilized and reconsittuted in artificial cerebrospinal fluid (CSF). Intracerebroventricular (IVT), but not subcutaneous, administration of this material to rats produced diuresis, natriuresis and kaliuresis in the following 8 h daylight period. During the overnight cycle, consummatory behavior and excretion of sodium and potassium were reduced. Similar responses were obtained after IVT administration of cerebellar cortex (CB) or large amounts of plasma. SFO, CB and cerebral cortex (CC) were incubated in potassium-enriched CSF to enhance release of secretory products. Urine volume was increased 8 h after IVT injection of SFO media; in the overnight cycle, food consumption, absolute urinary sodium and potassium, and [Na+-a1 were reduced. These effects were not produced by IVT injection of CC or CB media, or equal amounts of plasma proteins. Additional experiments demonstrated that choroid plexi and SFO effects were similar and that the active SFO material was dialyzable and thermal stable. These data suggest that SFO contains a water-soluble substance which is released into a posassium-enriched medium. The material is heat stable, has a relatively low molecular weight, and alters salt-water balance after injection into ventricular cerebrospinal fluid.", "contents": "Effects of subfornical organ extracts of salt-water balance in the rat. The subfornical organ (SFO) is regarded as a neurosecretory structure but no information is available on the nature or biological effects of the secretory products(s). Supernatants of water homogenates of rat SFO were lyophilized and reconsittuted in artificial cerebrospinal fluid (CSF). Intracerebroventricular (IVT), but not subcutaneous, administration of this material to rats produced diuresis, natriuresis and kaliuresis in the following 8 h daylight period. During the overnight cycle, consummatory behavior and excretion of sodium and potassium were reduced. Similar responses were obtained after IVT administration of cerebellar cortex (CB) or large amounts of plasma. SFO, CB and cerebral cortex (CC) were incubated in potassium-enriched CSF to enhance release of secretory products. Urine volume was increased 8 h after IVT injection of SFO media; in the overnight cycle, food consumption, absolute urinary sodium and potassium, and [Na+-a1 were reduced. These effects were not produced by IVT injection of CC or CB media, or equal amounts of plasma proteins. Additional experiments demonstrated that choroid plexi and SFO effects were similar and that the active SFO material was dialyzable and thermal stable. These data suggest that SFO contains a water-soluble substance which is released into a posassium-enriched medium. The material is heat stable, has a relatively low molecular weight, and alters salt-water balance after injection into ventricular cerebrospinal fluid.", "PMID": 953751}
{"id": "pubmed23n0032_720", "title": "Regional concentrations of noradrenaline and dopamine in rat brain.", "content": "The concentrations of noradrenaline and lopamine of 92 brain regions have been measured by a radiometric method which enabled discrimination between noradrenaline and adrenaline. Almost all brain regions investigated contained both noradrenaline and lopamine in measurable amount. However, both catecholamines appeared to be unevenly distributed. Very high dopamine concentrations were measured in the olfactory tubercle, the nucleus accumbens, the caudate nucleus and the rostral part of the medial forebrain bundle; the globus pallidus, the nucleus tractus diagonalis and the nucleus septalis lateralis were also very rich in dopamine. Outside the telencephalon the dopamine concentrations were rather low, except in the median eminence and the area tegmentalis ventralis (Tsai), an area corresponding to the A10 region. High noradrenaline concentrations were measured in most hypothalamic nuclei. Relatively high concentrations of this catecholamine were also measured in several mesencephalic (the ventral part of the central gray, the nucleus raphe dorsalis and the nucleus cuneiformis) and pontine (the locus coeruleus and the nuclei parabrachiales) regions. The highest noradrenaline concentrations in the medulla oblongata were observed in the A2 region and the nucleus commissuralis, which contained at least twice as much noradrenaline as did the more rostral part of the nucleus tractus solitarii.", "contents": "Regional concentrations of noradrenaline and dopamine in rat brain. The concentrations of noradrenaline and lopamine of 92 brain regions have been measured by a radiometric method which enabled discrimination between noradrenaline and adrenaline. Almost all brain regions investigated contained both noradrenaline and lopamine in measurable amount. However, both catecholamines appeared to be unevenly distributed. Very high dopamine concentrations were measured in the olfactory tubercle, the nucleus accumbens, the caudate nucleus and the rostral part of the medial forebrain bundle; the globus pallidus, the nucleus tractus diagonalis and the nucleus septalis lateralis were also very rich in dopamine. Outside the telencephalon the dopamine concentrations were rather low, except in the median eminence and the area tegmentalis ventralis (Tsai), an area corresponding to the A10 region. High noradrenaline concentrations were measured in most hypothalamic nuclei. Relatively high concentrations of this catecholamine were also measured in several mesencephalic (the ventral part of the central gray, the nucleus raphe dorsalis and the nucleus cuneiformis) and pontine (the locus coeruleus and the nuclei parabrachiales) regions. The highest noradrenaline concentrations in the medulla oblongata were observed in the A2 region and the nucleus commissuralis, which contained at least twice as much noradrenaline as did the more rostral part of the nucleus tractus solitarii.", "PMID": 953752}
{"id": "pubmed23n0032_721", "title": "Some effects of undernutrition on synaptic development -- a quantitative ultrastructural study.", "content": "The development of synaptic junctions in rat occipital cortex has been studied at 7 and 20 days postnatal in control and undernourished animals. In addition, adult control cortex has been compared with cortex derived from animals undernourished until 35 days postnatal and then nutritionally rehabilitated. Emphasis has been placed on material stained with phosphotungstic acid (E-PTA technique) although osmicated material has been examined. The body weights of the undernourished animals were lower than those of their age-matched controls, the deficit at 20 days being 55%. The number of synaptic junctions per unit area of the molecular layer was lower in the undernourished animals at 7 and 20 days, the most prominent deficit being at 20 days. In an attempt to detect ultrastructural correlates of undernutrition, E-PTA stained synaptic junctions were divided into 5 types (A-E), based upon their presynaptic characteristics are reflecting varying degrees of maturity. During normal development the immature synaptic types, particularly type E, are predominant, giving way to the more mature types (A-C) as development proceeds. Undernutrition however, appears to disrupt this developmental sequence, with a higher than normal percentage of immature synaptic types present at the age studied. Nutritional rehabilitation rectifies the imbalance to a degree, the adult rehabilitated pattern resembling the 20-day control pattern. Preliminary examination of the 20-day osmicated material confirmed the deficit of synapses per area of tissue in the undernourished cortex. It  also revealed decreases in the number of vesicles per terminal unit area and in the internal area of the synaptic vesicles in the undernourished tissue.", "contents": "Some effects of undernutrition on synaptic development -- a quantitative ultrastructural study. The development of synaptic junctions in rat occipital cortex has been studied at 7 and 20 days postnatal in control and undernourished animals. In addition, adult control cortex has been compared with cortex derived from animals undernourished until 35 days postnatal and then nutritionally rehabilitated. Emphasis has been placed on material stained with phosphotungstic acid (E-PTA technique) although osmicated material has been examined. The body weights of the undernourished animals were lower than those of their age-matched controls, the deficit at 20 days being 55%. The number of synaptic junctions per unit area of the molecular layer was lower in the undernourished animals at 7 and 20 days, the most prominent deficit being at 20 days. In an attempt to detect ultrastructural correlates of undernutrition, E-PTA stained synaptic junctions were divided into 5 types (A-E), based upon their presynaptic characteristics are reflecting varying degrees of maturity. During normal development the immature synaptic types, particularly type E, are predominant, giving way to the more mature types (A-C) as development proceeds. Undernutrition however, appears to disrupt this developmental sequence, with a higher than normal percentage of immature synaptic types present at the age studied. Nutritional rehabilitation rectifies the imbalance to a degree, the adult rehabilitated pattern resembling the 20-day control pattern. Preliminary examination of the 20-day osmicated material confirmed the deficit of synapses per area of tissue in the undernourished cortex. It  also revealed decreases in the number of vesicles per terminal unit area and in the internal area of the synaptic vesicles in the undernourished tissue.", "PMID": 953761}
{"id": "pubmed23n0032_722", "title": "The fine structure of the axons and growth cones of the human fetal cerebral cortex.", "content": "The fine structure of axon cylinders and growth cones has been examined in serial sections through the marginal zone of the human cerebral cortex from 10 to 22 weeks of gestation. Neurotubules, mitochondria, agranular reticulum, coated vesicles, and synaptic vesicles both of the translucent and dense core variety are found throughout the axon cylinders. At periodic intervals along the axons, bulged-out regions display accumulations of either synaptic vesicles or transversely oriented neurotubules. Prominent, growth cone varicosities are encountered at the distal segment of the axon cylinders. In these, mitochondria, channels of both tubular and dilated agranular reticulum, dense core vesicles, and multivesicular bodies lie in a finely filamentous matrix. Processes characterized by their content of either agranular reticulum and synaptic vesicles or a finely filamentous meshwork are infrequently continuous with these varicosities. Dense membrane thickenings are associated with some varicosities and their processes. The ultrastructural features of the human fetal growth cones have been compared to growth cones previously described in other mammalian and submammalian species.", "contents": "The fine structure of the axons and growth cones of the human fetal cerebral cortex. The fine structure of axon cylinders and growth cones has been examined in serial sections through the marginal zone of the human cerebral cortex from 10 to 22 weeks of gestation. Neurotubules, mitochondria, agranular reticulum, coated vesicles, and synaptic vesicles both of the translucent and dense core variety are found throughout the axon cylinders. At periodic intervals along the axons, bulged-out regions display accumulations of either synaptic vesicles or transversely oriented neurotubules. Prominent, growth cone varicosities are encountered at the distal segment of the axon cylinders. In these, mitochondria, channels of both tubular and dilated agranular reticulum, dense core vesicles, and multivesicular bodies lie in a finely filamentous matrix. Processes characterized by their content of either agranular reticulum and synaptic vesicles or a finely filamentous meshwork are infrequently continuous with these varicosities. Dense membrane thickenings are associated with some varicosities and their processes. The ultrastructural features of the human fetal growth cones have been compared to growth cones previously described in other mammalian and submammalian species.", "PMID": 953762}
{"id": "pubmed23n0032_723", "title": "Preparation of pure neuronal and non-neuronal cultures from embryonic chick sympathetic ganglia: a new method based on both differential cell adhesiveness and the formation of homotypic neuronal aggregates.", "content": "A new method has been developed for the preparation of essentially pure primary cultures of neurons and non-neuronal cells from 11-day embryonic chick sympathetic ganglia. This method utilizes (1) differences in cell-to-substrate adhesiveness between neurons and non-neuronal cells and (2) the capacity of neurons to form homotypic aggragates. The maximum difference in adhesiveness between neuronal and non-neuronal cells occurred when the ganglia were dissociated with trypsin following collection in a salt solution lacking divalent cations. This difference allowed the preparation of highly purified non-neuronal cultures and 85-90% pure neuronal cultures. Intermittent agitation during the period of cell separation markedly increased the purity of the neuronal cultures by (1) inhibiting neuronal but not non-neuronal cell attachment and (2) facilitating the formation of homotypic neuronal aggregates in the supernatant. Neuronal and non-neuronal cultures prepared under these conditions were more than 99% pure on the basis of both morphological and biochemical analyses. Both cell types exhibited attachment efficiencies greater than 95% and have been maintained for several weeks in vitro. Thus, completely isolated neuronal and non-neuronal cultures can be prepared and maintained for prolonged periods in the absence of cells of the other type.", "contents": "Preparation of pure neuronal and non-neuronal cultures from embryonic chick sympathetic ganglia: a new method based on both differential cell adhesiveness and the formation of homotypic neuronal aggregates. A new method has been developed for the preparation of essentially pure primary cultures of neurons and non-neuronal cells from 11-day embryonic chick sympathetic ganglia. This method utilizes (1) differences in cell-to-substrate adhesiveness between neurons and non-neuronal cells and (2) the capacity of neurons to form homotypic aggragates. The maximum difference in adhesiveness between neuronal and non-neuronal cells occurred when the ganglia were dissociated with trypsin following collection in a salt solution lacking divalent cations. This difference allowed the preparation of highly purified non-neuronal cultures and 85-90% pure neuronal cultures. Intermittent agitation during the period of cell separation markedly increased the purity of the neuronal cultures by (1) inhibiting neuronal but not non-neuronal cell attachment and (2) facilitating the formation of homotypic neuronal aggregates in the supernatant. Neuronal and non-neuronal cultures prepared under these conditions were more than 99% pure on the basis of both morphological and biochemical analyses. Both cell types exhibited attachment efficiencies greater than 95% and have been maintained for several weeks in vitro. Thus, completely isolated neuronal and non-neuronal cultures can be prepared and maintained for prolonged periods in the absence of cells of the other type.", "PMID": 953763}
{"id": "pubmed23n0032_724", "title": "Effects of substance P on functionally identified units in cat spinal cord.", "content": "A correlation was made between the effects of synthetic substance P (ssP) on spinal dorsal horn neurones and the responses of these neurones to natural peripheral stimulation. It was found that ssP caused excitation only of those units which were excited by noxious radiant heat applied to the skin. ssP also caused a small facilitation of the excitatory response to this noxious stimulus and, in two cases, led to a response to noxious heat of units which had previously been unaffected by this stimulus. The ratio of ssP sensitive to insensitive units was highest in the deeper parts of the dorsal horn. Excitation by ssP showed a positive correlation to excitation by bradykinin. These results suggest that substance P may be involved in excitatory spinal processes and that its actions may be associated specifically with nociception.", "contents": "Effects of substance P on functionally identified units in cat spinal cord. A correlation was made between the effects of synthetic substance P (ssP) on spinal dorsal horn neurones and the responses of these neurones to natural peripheral stimulation. It was found that ssP caused excitation only of those units which were excited by noxious radiant heat applied to the skin. ssP also caused a small facilitation of the excitatory response to this noxious stimulus and, in two cases, led to a response to noxious heat of units which had previously been unaffected by this stimulus. The ratio of ssP sensitive to insensitive units was highest in the deeper parts of the dorsal horn. Excitation by ssP showed a positive correlation to excitation by bradykinin. These results suggest that substance P may be involved in excitatory spinal processes and that its actions may be associated specifically with nociception.", "PMID": 953765}
{"id": "pubmed23n0032_725", "title": "Neuronal stimulation of (3H)thymidine incorporation by primary cultures of highly purified non-neuronal cells.", "content": "A specific intercellular interaction has been demonstrated between neuronal and non-neuronal cells that appears to increase the rate of non-neuronal cell proliferation. Isolated and recombined primary cultures of both cell types were prepared from 11-day embryonic chick sympathetic ganglia by a method recently developed in this laboratory. When non-dividing neurons were added to an equal number of proliferating non-neuronal cells, the amount of [methyl-3H]thymidine incorporated by these mixed cultures was 230% greater than that incorporated by 99% pure non-neuronal cultures. Removal of all neurons from such non-neuronal cultures by a 48-h preincubation without nerve growth factor resulted in an even greater increase in [3H]thymidine incorporation upon addition of neurons (370%). When increasing numbers of isolated neurons were added to non-neuronal cell cultures, the amount of [3H]thymidine incorporation initially increased in a dose-dependent fashion until it reached a plateau. In contrast, the addition of increasing numbers of non-neuronal cells to a constant number of neurons resulted in a linear increase in [3H]thymidine incorporation. In some cases neurons and non-neuronal cells were not grown in direct physical contact but were only allowed to communicate with one another through the culture medium. Such indirect communication never resulted in a stimulation of [3H]thymidine incorporation. When neurons were added to cultures of embryonic chick fibroblasts, the neurons grew well but did not stimulate [3H]thymidine incorporation by the fibroblasts. These results suggest that embryonic sympathetic neurons selectively stimulate the proliferation of non-neuronal cells derived from the same source.", "contents": "Neuronal stimulation of (3H)thymidine incorporation by primary cultures of highly purified non-neuronal cells. A specific intercellular interaction has been demonstrated between neuronal and non-neuronal cells that appears to increase the rate of non-neuronal cell proliferation. Isolated and recombined primary cultures of both cell types were prepared from 11-day embryonic chick sympathetic ganglia by a method recently developed in this laboratory. When non-dividing neurons were added to an equal number of proliferating non-neuronal cells, the amount of [methyl-3H]thymidine incorporated by these mixed cultures was 230% greater than that incorporated by 99% pure non-neuronal cultures. Removal of all neurons from such non-neuronal cultures by a 48-h preincubation without nerve growth factor resulted in an even greater increase in [3H]thymidine incorporation upon addition of neurons (370%). When increasing numbers of isolated neurons were added to non-neuronal cell cultures, the amount of [3H]thymidine incorporation initially increased in a dose-dependent fashion until it reached a plateau. In contrast, the addition of increasing numbers of non-neuronal cells to a constant number of neurons resulted in a linear increase in [3H]thymidine incorporation. In some cases neurons and non-neuronal cells were not grown in direct physical contact but were only allowed to communicate with one another through the culture medium. Such indirect communication never resulted in a stimulation of [3H]thymidine incorporation. When neurons were added to cultures of embryonic chick fibroblasts, the neurons grew well but did not stimulate [3H]thymidine incorporation by the fibroblasts. These results suggest that embryonic sympathetic neurons selectively stimulate the proliferation of non-neuronal cells derived from the same source.", "PMID": 953764}
{"id": "pubmed23n0032_726", "title": "Alteration of the pattern of hippocampal nerve cell RNA labelling during training in rats.", "content": "RNA synthesis in the CA3 region of brain hippocampus was studied in rats trained to a handedness reversal task. The newly synthesized RNA, labelled by intraventricular injection of radioactive orotic acid, was extracted and analyzed by micromethods. The result was following. In the trained animals the incorporation of the labelled precursor into RNA, corrected by the pool radioactivity, was almost double in comparison with the controls. In the trained animals there was a stimulation of the incorporation in the high molecular weight (greater than 18S) RNA region and in two specific low molecular weight regions: 8-9 and 16-17S. Taking in account previous results of other authors, these data appear to imply a stimulation of brain mRNA synthesis as a result of training.", "contents": "Alteration of the pattern of hippocampal nerve cell RNA labelling during training in rats. RNA synthesis in the CA3 region of brain hippocampus was studied in rats trained to a handedness reversal task. The newly synthesized RNA, labelled by intraventricular injection of radioactive orotic acid, was extracted and analyzed by micromethods. The result was following. In the trained animals the incorporation of the labelled precursor into RNA, corrected by the pool radioactivity, was almost double in comparison with the controls. In the trained animals there was a stimulation of the incorporation in the high molecular weight (greater than 18S) RNA region and in two specific low molecular weight regions: 8-9 and 16-17S. Taking in account previous results of other authors, these data appear to imply a stimulation of brain mRNA synthesis as a result of training.", "PMID": 953766}
{"id": "pubmed23n0032_727", "title": "Effects of fructose-1,6-diphosphate administration on learning efficiency and time sense of the honey bee, Apis mellifica carnica.", "content": "In discrimination experiments honey bees had to decide between two glass dishes, one placed on a black cross, the other 40 cm away on a black square. In spontaneous selections the bees in 70% of all cases chose the black cross. The bees were then trained to search back and forth between the cross and the square until after 5 min sugar-water was given on the black square. Over a total of 5 learning trials, during which multiple decisions had been made by the searching insects, 96% of all choices were in favour of the black square. If 80 mM fructose-1,6-diphosphate (F-1,6-P2) in 1 M glucose had been ingested by the bees 30 min before the first spontaneous choice, learning was facilitated with 83% deciding in favour of the black square at the first trial compared with 46% of the controls (which had been fed on 1 M glucose only). In both groups of bees the same number of flight approches was made to the cross or square as made by the bees searching for 5 min before the reward was given. Thus no change in general activity or harvesting motivation appears to be induced by F-1,6-P2. When F-1,6-P2 had been given at the end of a successful learning series the bees favoured the black square for up to 3 days. In contrast to the controls no new incentive needed to be given during this period. Ingestion of other metabolites proved either ineffective, as in the case of fructose-6-phosphate or 5'-adenosine monophosphate, or, as observed after feeding citrate plus 3-phosphoglycerate, even reduced the performance. To test the effect of F-1,6P2 on the same time sense (circadian rhythm), bees were trained on three successive days to visit a feeding place at a specific time of day. The control bees which ingested only glucose on the evening of the 3rd day returned at their entrained 24-h interval on the 4th day. In contrast, the maximum frequency of appearance of bees fed on F-1,6-P2 was advanced by one hour, with minor appearance peaks at earlier hours of the day.", "contents": "Effects of fructose-1,6-diphosphate administration on learning efficiency and time sense of the honey bee, Apis mellifica carnica. In discrimination experiments honey bees had to decide between two glass dishes, one placed on a black cross, the other 40 cm away on a black square. In spontaneous selections the bees in 70% of all cases chose the black cross. The bees were then trained to search back and forth between the cross and the square until after 5 min sugar-water was given on the black square. Over a total of 5 learning trials, during which multiple decisions had been made by the searching insects, 96% of all choices were in favour of the black square. If 80 mM fructose-1,6-diphosphate (F-1,6-P2) in 1 M glucose had been ingested by the bees 30 min before the first spontaneous choice, learning was facilitated with 83% deciding in favour of the black square at the first trial compared with 46% of the controls (which had been fed on 1 M glucose only). In both groups of bees the same number of flight approches was made to the cross or square as made by the bees searching for 5 min before the reward was given. Thus no change in general activity or harvesting motivation appears to be induced by F-1,6-P2. When F-1,6-P2 had been given at the end of a successful learning series the bees favoured the black square for up to 3 days. In contrast to the controls no new incentive needed to be given during this period. Ingestion of other metabolites proved either ineffective, as in the case of fructose-6-phosphate or 5'-adenosine monophosphate, or, as observed after feeding citrate plus 3-phosphoglycerate, even reduced the performance. To test the effect of F-1,6P2 on the same time sense (circadian rhythm), bees were trained on three successive days to visit a feeding place at a specific time of day. The control bees which ingested only glucose on the evening of the 3rd day returned at their entrained 24-h interval on the 4th day. In contrast, the maximum frequency of appearance of bees fed on F-1,6-P2 was advanced by one hour, with minor appearance peaks at earlier hours of the day.", "PMID": 953767}
{"id": "pubmed23n0032_728", "title": "Monoamine metabolism in the developing rat brain and effects of ionizing radiation.", "content": "Neonatal X-radiation induces profound changes in monoamine metabolism in the developing CNS. NE and 5-HT concentrations increase 7 days post-radiation in all CNS regions undergoing rapid axonal growth and proliferation, but not in the region of the cell bodies from which the respective neurotransmitters originate. The increase in NE and 5-HT levels is accompanied by a concomitant increase in the rate of synthesis. While these changes are evident as late as 22 days of age, the monoaminergic systems revert to normal by maturity. It is suggested that these alterations reflect an imbalance in the density of nerve endings to the region where these terminate. These regions are immature at birth and cell proliferation, a process which is affected by X-radiation, is still occurring at the time of exposure.", "contents": "Monoamine metabolism in the developing rat brain and effects of ionizing radiation. Neonatal X-radiation induces profound changes in monoamine metabolism in the developing CNS. NE and 5-HT concentrations increase 7 days post-radiation in all CNS regions undergoing rapid axonal growth and proliferation, but not in the region of the cell bodies from which the respective neurotransmitters originate. The increase in NE and 5-HT levels is accompanied by a concomitant increase in the rate of synthesis. While these changes are evident as late as 22 days of age, the monoaminergic systems revert to normal by maturity. It is suggested that these alterations reflect an imbalance in the density of nerve endings to the region where these terminate. These regions are immature at birth and cell proliferation, a process which is affected by X-radiation, is still occurring at the time of exposure.", "PMID": 953768}
{"id": "pubmed23n0032_729", "title": "Isolation of synaptic vesicles from Narcine brasiliensis electric organ: some influences on release of vesicular acetylcholine and ATP.", "content": "A simple density gradient centrifugation technique for separating electric organ cholinergic synaptic vesicles from other organelles and membrane fragments is described. Frozen, ground electric organ is extracted with a solution of similar density to the vesicles; during the subsequent centrifugation, vesicles remain suspended in the extraction medium and heavier contaminating structures sediment out. In confirmation of results obtained with mammalian central nervous system vesicles, a biphasic pattern of efflux of bound ACh is demonstrated. Low levels of phospholipase A2 (EC 3.1.1.4.) induce efflux of ACh from the vesicle fraction; it is shown that the concomitant fall in vesicle bound ATP is due to ATP efflux rather than ATP hydrolysis within the vesicle.", "contents": "Isolation of synaptic vesicles from Narcine brasiliensis electric organ: some influences on release of vesicular acetylcholine and ATP. A simple density gradient centrifugation technique for separating electric organ cholinergic synaptic vesicles from other organelles and membrane fragments is described. Frozen, ground electric organ is extracted with a solution of similar density to the vesicles; during the subsequent centrifugation, vesicles remain suspended in the extraction medium and heavier contaminating structures sediment out. In confirmation of results obtained with mammalian central nervous system vesicles, a biphasic pattern of efflux of bound ACh is demonstrated. Low levels of phospholipase A2 (EC 3.1.1.4.) induce efflux of ACh from the vesicle fraction; it is shown that the concomitant fall in vesicle bound ATP is due to ATP efflux rather than ATP hydrolysis within the vesicle.", "PMID": 953769}
{"id": "pubmed23n0032_730", "title": "Phosphorus deficiency, parathyroid hormone and bone resorption in the growing rat.", "content": "Sham-operated and parathyroidectomized (PTX) rats were divided into two pair-fed groups, one on a normal mineral intake (0.5% Ca, 0.3% P), the other on a regimen low in phosphorus (0.5% Ca, 0.03% P). P depletion led to a drop in plasma P and urine P, a rise in plasma Ca and a marked rise in urine Ca, a drop in serum magnesium and a rise in urine Mg. The changes were more pronounced in the PTX animals, but final values were the same in both groups. Parallel bone-seeking isotope (85Sr, 177Lu, 237Np) studies in nonablated animals revealed an increase in the urinary nuclide output and in the urine/tibia ratio in P-deficient animals. Normal and primary bone osteocytes decreased and enlarged osteocytes increased as a result of P deficiency; osteoclasts and osteoblasts also increased. Bone composition showed a drop in ash content and a rise in water, with a light decrease in both Ca and P, and a corresponding rise in hydroxyproline and nitrogen in the P-deficient animals. The results are interpreted to mean that P-deficiency in the young growing rat leads to an increase in bone resorption which occurs also in the absence of parathyroid hormone (PTH). The fact that final values were similar in the control and PTX P-deficient animals suggests that steady-state regulation can also occur without PTH. Because P-deficiency leads to rapid hypercalcemia and rapid marked hypercalciuria, there may exist a mechanism for phosphate regulation which would then supersede Ca homeostasis. The change in serum and urine Mg levels may reflect a decrease in tubular Ca and Mg reabsorption associated with P-deficiency.", "contents": "Phosphorus deficiency, parathyroid hormone and bone resorption in the growing rat. Sham-operated and parathyroidectomized (PTX) rats were divided into two pair-fed groups, one on a normal mineral intake (0.5% Ca, 0.3% P), the other on a regimen low in phosphorus (0.5% Ca, 0.03% P). P depletion led to a drop in plasma P and urine P, a rise in plasma Ca and a marked rise in urine Ca, a drop in serum magnesium and a rise in urine Mg. The changes were more pronounced in the PTX animals, but final values were the same in both groups. Parallel bone-seeking isotope (85Sr, 177Lu, 237Np) studies in nonablated animals revealed an increase in the urinary nuclide output and in the urine/tibia ratio in P-deficient animals. Normal and primary bone osteocytes decreased and enlarged osteocytes increased as a result of P deficiency; osteoclasts and osteoblasts also increased. Bone composition showed a drop in ash content and a rise in water, with a light decrease in both Ca and P, and a corresponding rise in hydroxyproline and nitrogen in the P-deficient animals. The results are interpreted to mean that P-deficiency in the young growing rat leads to an increase in bone resorption which occurs also in the absence of parathyroid hormone (PTH). The fact that final values were similar in the control and PTX P-deficient animals suggests that steady-state regulation can also occur without PTH. Because P-deficiency leads to rapid hypercalcemia and rapid marked hypercalciuria, there may exist a mechanism for phosphate regulation which would then supersede Ca homeostasis. The change in serum and urine Mg levels may reflect a decrease in tubular Ca and Mg reabsorption associated with P-deficiency.", "PMID": 953782}
{"id": "pubmed23n0032_731", "title": "Maintenance by cortisone of the calcemic response to parathyroid extract of rats on a diet without vitamin D and low in calcium.", "content": "Weanling rats raised for 21 days on a vitamin D deprived, low Ca diet (0.02%), and given chronic cortisone administration (5 mg/kg/d), maintain responsiveness to the hypercalcemic effect of endogenous and exogenous parathyroid extract (PTE). The PTE action is a bone effect that does not require the presence of the kidneys, and is not related to a higher concentration of calcium or cortisone. Maintained sensitivity of D- Ca- Cort+ rats to PTE does not appear to be the consequence of a lesser degree of D-deficiency: the whole body vitamin D pool and its chloroform-soluble fraction in these animals are not different from those of their D- Ca- PTE- unresponsive controls. Repeated PTE injections for 4 days exhaust the sensitivity to the hypercalcemic action of PTE of D- Ca- Cort+ rats. The present data seem to indicate that cortisone-treated D- Ca- rats, responsive to the bone action of PTE, are characterized by a near normal bone calcium content and Ca/P ratio, and a significant increase in the number of osteoclasts.", "contents": "Maintenance by cortisone of the calcemic response to parathyroid extract of rats on a diet without vitamin D and low in calcium. Weanling rats raised for 21 days on a vitamin D deprived, low Ca diet (0.02%), and given chronic cortisone administration (5 mg/kg/d), maintain responsiveness to the hypercalcemic effect of endogenous and exogenous parathyroid extract (PTE). The PTE action is a bone effect that does not require the presence of the kidneys, and is not related to a higher concentration of calcium or cortisone. Maintained sensitivity of D- Ca- Cort+ rats to PTE does not appear to be the consequence of a lesser degree of D-deficiency: the whole body vitamin D pool and its chloroform-soluble fraction in these animals are not different from those of their D- Ca- PTE- unresponsive controls. Repeated PTE injections for 4 days exhaust the sensitivity to the hypercalcemic action of PTE of D- Ca- Cort+ rats. The present data seem to indicate that cortisone-treated D- Ca- rats, responsive to the bone action of PTE, are characterized by a near normal bone calcium content and Ca/P ratio, and a significant increase in the number of osteoclasts.", "PMID": 953783}
{"id": "pubmed23n0032_732", "title": "Assay of bone resorption in vivo with 3H-tetracycline.", "content": "3H-Tetracycline (3H-TC) was used to quantify resorption in whole bones of growing rats and dogs. After repeated isotopic labeling of actively growing embryos or neonates, 3H-TC was observed to be distributed homogeneously and in equilibrium with 45Ca. A rapid and large loss of 3H-TC and a small loss of 45Ca occurred during the early weeks of rapid bone growth, suggesting that absolute amounts of 45Ca resorbed from bone, as reflected by losses of 3H-TC, are five to ten times greater than the net amounts of 45Ca lost from bone. Minimal loss of 3H-TC occurred due to nonspecific physicochemical exchange in vivo or in vitro (5%) except with nonradioactive tetracycline, and 3H-TC was not greatly exchanged or reused (10%) in vivo. The data are considered in terms of local and systemic conservation of calcium.", "contents": "Assay of bone resorption in vivo with 3H-tetracycline. 3H-Tetracycline (3H-TC) was used to quantify resorption in whole bones of growing rats and dogs. After repeated isotopic labeling of actively growing embryos or neonates, 3H-TC was observed to be distributed homogeneously and in equilibrium with 45Ca. A rapid and large loss of 3H-TC and a small loss of 45Ca occurred during the early weeks of rapid bone growth, suggesting that absolute amounts of 45Ca resorbed from bone, as reflected by losses of 3H-TC, are five to ten times greater than the net amounts of 45Ca lost from bone. Minimal loss of 3H-TC occurred due to nonspecific physicochemical exchange in vivo or in vitro (5%) except with nonradioactive tetracycline, and 3H-TC was not greatly exchanged or reused (10%) in vivo. The data are considered in terms of local and systemic conservation of calcium.", "PMID": 953784}
{"id": "pubmed23n0032_733", "title": "The effect of magnesium and fluoride on nephrocalcinosis and aortic calcification in rats given high sucrose diets with added phospnates.", "content": "The study was conducted to observe in rats the possible modification of ectopic calcification by magnesium-orthophosphate-fluoride combinations, used as additives of diet for reduction of the cariogenicity of the sucrose. In rats, fed low magnesium diets, extra dietary orthophosphate (2%) considerably elevated the calcification of kidneys. Further additions of magnesium and fluoride partially reduced this adverse effect of phosphate. While the calcium content of the aorta in rats, fed low magnesium-high phosphate diet, was considerably elevated, the further addition of magnesium (40 ppm) partially reduced the calcifying effect of phosphate in aorta. Fluoride (15 ppm) together with magnesium (40 ppm) completely reduced it. The appearance of renal calculi caused by a low magnesium diet or by extra phosphate were similar according to light and electron microscopy except for the larger size in the latter case and occasional extratubular calculi found in groups with high phosphate-low magnesium and high phosphate with added magnesium diets.", "contents": "The effect of magnesium and fluoride on nephrocalcinosis and aortic calcification in rats given high sucrose diets with added phospnates. The study was conducted to observe in rats the possible modification of ectopic calcification by magnesium-orthophosphate-fluoride combinations, used as additives of diet for reduction of the cariogenicity of the sucrose. In rats, fed low magnesium diets, extra dietary orthophosphate (2%) considerably elevated the calcification of kidneys. Further additions of magnesium and fluoride partially reduced this adverse effect of phosphate. While the calcium content of the aorta in rats, fed low magnesium-high phosphate diet, was considerably elevated, the further addition of magnesium (40 ppm) partially reduced the calcifying effect of phosphate in aorta. Fluoride (15 ppm) together with magnesium (40 ppm) completely reduced it. The appearance of renal calculi caused by a low magnesium diet or by extra phosphate were similar according to light and electron microscopy except for the larger size in the latter case and occasional extratubular calculi found in groups with high phosphate-low magnesium and high phosphate with added magnesium diets.", "PMID": 953785}
{"id": "pubmed23n0032_734", "title": "The distribution of water in calcified turkey leg tendon.", "content": "X-ray diffraction and water sorption data are presented which show that the extracellular water in calcified turkey leg tendon is associated principally with the collagen component.", "contents": "The distribution of water in calcified turkey leg tendon. X-ray diffraction and water sorption data are presented which show that the extracellular water in calcified turkey leg tendon is associated principally with the collagen component.", "PMID": 953786}
{"id": "pubmed23n0032_735", "title": "Bone resorption measurement with unusual bone markers: critical evaluation of the method in phosphorus-deficient and calcium-deficient growing rats.", "content": "An in vivo method to evaluate bone resorption in rats, by using unusual bone seekers not dependent on renal tubular transfer, is described and a critical evaluation of the method is made. In our experimental conditions, 85Sr and 177Lu are virtually exclusively localized in bone whereas 237Np remains unchanged in different soft organs, so that the concomitant use of these markers can be used for measuring bone resorption. If osteolysis occurs 21 days after the injection of these markers, under our experimental conditions, any increase in the urinary excretion of 177Lu and 237Np represents a rise in bone resorption, whereas an increase in Sr excretion reflects both and renal tubular events. According to our bone localization studies, the enhancement of Lu and Np excretion reflects primarily an increase in cortical bone resorption localized at the endosteal (Lu) and at the periosteal (Np) surfaces respectively. In addition, strontium is considered to be the marker of mineral resorption whereas Lu and Np, under our experimental conditions, would reflect the organic bone resorption. This method is tested in phosphorus-deficient rats and in calcium-deficient rats which exhibit disturbances of calcium metabolism at both the bone and kidney levels. In agreement with previous investigations, the use of these bone markers to evaluate osteolysis shows: (a) after a 1-week phosphorus deficiency, a slight increase in cortical bone resorption with a simultaneous fall in calcium and strontium renal tubular reabsorption, and (b) after a 1-week calcium deficiency, a high rise in cortical bone resorption with a simultaneous increase in the renal tubular reabsorption of calcium and strontium.", "contents": "Bone resorption measurement with unusual bone markers: critical evaluation of the method in phosphorus-deficient and calcium-deficient growing rats. An in vivo method to evaluate bone resorption in rats, by using unusual bone seekers not dependent on renal tubular transfer, is described and a critical evaluation of the method is made. In our experimental conditions, 85Sr and 177Lu are virtually exclusively localized in bone whereas 237Np remains unchanged in different soft organs, so that the concomitant use of these markers can be used for measuring bone resorption. If osteolysis occurs 21 days after the injection of these markers, under our experimental conditions, any increase in the urinary excretion of 177Lu and 237Np represents a rise in bone resorption, whereas an increase in Sr excretion reflects both and renal tubular events. According to our bone localization studies, the enhancement of Lu and Np excretion reflects primarily an increase in cortical bone resorption localized at the endosteal (Lu) and at the periosteal (Np) surfaces respectively. In addition, strontium is considered to be the marker of mineral resorption whereas Lu and Np, under our experimental conditions, would reflect the organic bone resorption. This method is tested in phosphorus-deficient rats and in calcium-deficient rats which exhibit disturbances of calcium metabolism at both the bone and kidney levels. In agreement with previous investigations, the use of these bone markers to evaluate osteolysis shows: (a) after a 1-week phosphorus deficiency, a slight increase in cortical bone resorption with a simultaneous fall in calcium and strontium renal tubular reabsorption, and (b) after a 1-week calcium deficiency, a high rise in cortical bone resorption with a simultaneous increase in the renal tubular reabsorption of calcium and strontium.", "PMID": 953788}
{"id": "pubmed23n0032_736", "title": "Remineralization of dentin in vitro.", "content": "The remineralization of completely demineralized bovine dentin was studied in vitro by monitoring the velocities of fall of small particles through calcifying solutions. The density of a particle of dentin may be found from its velocity of fall through a fluid using Stokes' law. The minimum concentration product of calcium and acid phosphate ions of the solution in which remineralization would take place was 3.6 (mM)2 in the presence of 22 mM bicarbonate, pH 7.35, and ionic strength 0.1. This just above the solubility product of brushite (CaHPO4-2H2O). Incubation of decalcified dentin in a phosphoprotein removed from dentin during demineralization, or in phosvitin, had no effect on remineralization. The rates of remineralization and of the fraction remineralized were inversely proportional to particle size. This inverse correlation may be due to deposition of mineral in a surface layer of constant depth irrespective of particle size. The fraction of a particle remineralized was greatly increased by the use of highly supersaturated calcifying solutions or by the incorporation of fluoride into the solutions. The empirical reaction order of remineralization for both calcium and phosphate ions was found to be unity, which is, within the error limits, equal to the order of growth of seed crystals of hydroxyapatite in calcifying solutions of the same composition.", "contents": "Remineralization of dentin in vitro. The remineralization of completely demineralized bovine dentin was studied in vitro by monitoring the velocities of fall of small particles through calcifying solutions. The density of a particle of dentin may be found from its velocity of fall through a fluid using Stokes' law. The minimum concentration product of calcium and acid phosphate ions of the solution in which remineralization would take place was 3.6 (mM)2 in the presence of 22 mM bicarbonate, pH 7.35, and ionic strength 0.1. This just above the solubility product of brushite (CaHPO4-2H2O). Incubation of decalcified dentin in a phosphoprotein removed from dentin during demineralization, or in phosvitin, had no effect on remineralization. The rates of remineralization and of the fraction remineralized were inversely proportional to particle size. This inverse correlation may be due to deposition of mineral in a surface layer of constant depth irrespective of particle size. The fraction of a particle remineralized was greatly increased by the use of highly supersaturated calcifying solutions or by the incorporation of fluoride into the solutions. The empirical reaction order of remineralization for both calcium and phosphate ions was found to be unity, which is, within the error limits, equal to the order of growth of seed crystals of hydroxyapatite in calcifying solutions of the same composition.", "PMID": 953789}
{"id": "pubmed23n0032_737", "title": "Bone in dermatosparaxis. I. Morphologic analysis.", "content": "Normal (N-) calf bone consists of lamellae regularly spaced and oriented parallel to the periosteum. The lamellae increase in thickness from the periosteum to the medullary cavity, by apposition of layers of cells and a calcifying matrix on either side of a hypercalcified primer. In the dermatosparactic (D-) bone, the hypercalcified primer is barely visible and the cells are irregularly arranged within the lamellae. The poorly defined vascular spaces are partly filled with an acellular calcified material. In the D-bone, the collagen fibers are sparse and radiate from the vascular space, while in the N-bone they are abundant and laid down concentric with the blood channels. In the D-bone, only a few weeks old, the outer lamellae are radially oriented with respect to the medullar cavity, while haversian remodeling already occurs in the inner part of the diaphysis. At 6 months, the inner half of the diaphysis is made up of normal haversian secondary bone, while the outer half is made up of radial lamellae. The alteration of the mechanical properties of procollagen fibers in the D-bone might be responsible for its defective organization. A resistant fibrous framework, therefore, seems required to ensure the spatial organization of the cells in the calcifying matrix and to maintain its cohesion.", "contents": "Bone in dermatosparaxis. I. Morphologic analysis. Normal (N-) calf bone consists of lamellae regularly spaced and oriented parallel to the periosteum. The lamellae increase in thickness from the periosteum to the medullary cavity, by apposition of layers of cells and a calcifying matrix on either side of a hypercalcified primer. In the dermatosparactic (D-) bone, the hypercalcified primer is barely visible and the cells are irregularly arranged within the lamellae. The poorly defined vascular spaces are partly filled with an acellular calcified material. In the D-bone, the collagen fibers are sparse and radiate from the vascular space, while in the N-bone they are abundant and laid down concentric with the blood channels. In the D-bone, only a few weeks old, the outer lamellae are radially oriented with respect to the medullar cavity, while haversian remodeling already occurs in the inner part of the diaphysis. At 6 months, the inner half of the diaphysis is made up of normal haversian secondary bone, while the outer half is made up of radial lamellae. The alteration of the mechanical properties of procollagen fibers in the D-bone might be responsible for its defective organization. A resistant fibrous framework, therefore, seems required to ensure the spatial organization of the cells in the calcifying matrix and to maintain its cohesion.", "PMID": 953790}
{"id": "pubmed23n0032_738", "title": "Bone in dermatosparaxis. II. Chemical analysis.", "content": "Physical and chemical analysis of the organic matrix of dermatosparactic (D-) bone was performed to evaluate the implication of an alteration in the mechanical properties of procollagen fibers in the process of calcification. The D-diaphyseal bone contained less collagen and more hydroxyapatite than normal (N-) calf bone. Fractionated by centrifugation in a density gradient, D-bone was found to be composed of more than 80% weight of a highly calcified fraction while N-bone of the same age contained only 37% of this fraction. As compared to N-bone, the amount of organic matrix per unit volume of D-bone was increased in the least calcified fractions and reduced in the most mineralized ones. The newly formed matrix of D-bone contained an amount of collagen similar to the N-bone while the concentration of other glycoproteins was higher. Calcification was accompanied, in both types of bone, by a progressive reduction of glycoproteins and collagen; the diminution of collagen was higher in D- than in N-bone. The presence in D-bone of procollagen fibers with altered mechanical properties might be responsible for an excess of glycoproteins in the newly formed matrix. Calcium salt deposition in this matrix and progressive distension of the fibrous framework are two possible mechanisms by which hypercalcification of D-bone occurs.", "contents": "Bone in dermatosparaxis. II. Chemical analysis. Physical and chemical analysis of the organic matrix of dermatosparactic (D-) bone was performed to evaluate the implication of an alteration in the mechanical properties of procollagen fibers in the process of calcification. The D-diaphyseal bone contained less collagen and more hydroxyapatite than normal (N-) calf bone. Fractionated by centrifugation in a density gradient, D-bone was found to be composed of more than 80% weight of a highly calcified fraction while N-bone of the same age contained only 37% of this fraction. As compared to N-bone, the amount of organic matrix per unit volume of D-bone was increased in the least calcified fractions and reduced in the most mineralized ones. The newly formed matrix of D-bone contained an amount of collagen similar to the N-bone while the concentration of other glycoproteins was higher. Calcification was accompanied, in both types of bone, by a progressive reduction of glycoproteins and collagen; the diminution of collagen was higher in D- than in N-bone. The presence in D-bone of procollagen fibers with altered mechanical properties might be responsible for an excess of glycoproteins in the newly formed matrix. Calcium salt deposition in this matrix and progressive distension of the fibrous framework are two possible mechanisms by which hypercalcification of D-bone occurs.", "PMID": 953791}
{"id": "pubmed23n0032_739", "title": "Scanning electron microscopy of dental calculus.", "content": "The morphologic structure of anorganic dental calculus was studied by means of the scanning electron microscope. From surface observations, calculus is apparently composed of two components with distinguishable patters of calcification. One component is formed by the precipitation of minute calcific crystals on microorganisms and intermicrobial substances (plaque matrix). Such calcified masses, often spherical in shape, have a sponge-like appearance with empty spaces representing the former sites of entombed and degenerated organisms. Thus, intracellular calcification is not evident at this stage of calculus development. The other component, although having at least one common calcification front with the former, does not appear to be directly associated with microbial calcification. It exhibits a configuration of generally larger crystal growths of varying shapes and sizes. These two calcification patterns are comparable, both in distribution and size, to what has been observed by means of the transmission electron microscope, and what Schroeder has designated as \"types A & B centers of mineralization,\" respectively. The calcific precipitation in type A centers have been identified by X-ray diffraction as hydroxyapatite. It is, therefore, speculated that the crystal patters in type B centers might represent other known forms of calcium phosphates present in calculus, such as octacalcium phosphate, whitlockite and brushite.", "contents": "Scanning electron microscopy of dental calculus. The morphologic structure of anorganic dental calculus was studied by means of the scanning electron microscope. From surface observations, calculus is apparently composed of two components with distinguishable patters of calcification. One component is formed by the precipitation of minute calcific crystals on microorganisms and intermicrobial substances (plaque matrix). Such calcified masses, often spherical in shape, have a sponge-like appearance with empty spaces representing the former sites of entombed and degenerated organisms. Thus, intracellular calcification is not evident at this stage of calculus development. The other component, although having at least one common calcification front with the former, does not appear to be directly associated with microbial calcification. It exhibits a configuration of generally larger crystal growths of varying shapes and sizes. These two calcification patterns are comparable, both in distribution and size, to what has been observed by means of the transmission electron microscope, and what Schroeder has designated as \"types A & B centers of mineralization,\" respectively. The calcific precipitation in type A centers have been identified by X-ray diffraction as hydroxyapatite. It is, therefore, speculated that the crystal patters in type B centers might represent other known forms of calcium phosphates present in calculus, such as octacalcium phosphate, whitlockite and brushite.", "PMID": 953792}
{"id": "pubmed23n0032_740", "title": "Calcification of alpha-elastin coacervates: a bulk property of elastin.", "content": "Scanning electron microscopy and electron probe microanalysis studies are reported on thin sections of calcified coacervates of alpha-elastin. It is found that the capacity of elastin coacervates to initiate calcification is a bulk property of the coacervate and not limited to the serum-coacervate interface, that the calcium phosphate deposits act to bind the protein units together and slow the dissolution and spreading of the coacervate as it floats on an airwater interface, and that, within the limits of detectability, there is no involvement of sulfur. As the charged groups of alpha-elastin had been blocked, the initiation of deposition is due to neutral sites in the protein which are tightly bound to the calcium phosphate deposits.", "contents": "Calcification of alpha-elastin coacervates: a bulk property of elastin. Scanning electron microscopy and electron probe microanalysis studies are reported on thin sections of calcified coacervates of alpha-elastin. It is found that the capacity of elastin coacervates to initiate calcification is a bulk property of the coacervate and not limited to the serum-coacervate interface, that the calcium phosphate deposits act to bind the protein units together and slow the dissolution and spreading of the coacervate as it floats on an airwater interface, and that, within the limits of detectability, there is no involvement of sulfur. As the charged groups of alpha-elastin had been blocked, the initiation of deposition is due to neutral sites in the protein which are tightly bound to the calcium phosphate deposits.", "PMID": 953793}
{"id": "pubmed23n0032_741", "title": "Separation of potato spindle tuber viroid ribonucleic acid from Scopolia sinensis into three infectious forms and the purification and oligonucleotide pattern of fraction II RNA. Part IX.", "content": "The existence of three infectious forms of potato spindle tuber viroid (PSTV) RNA from Scopolia sinensis was demonstrated by fractionation with high salt, by reverse phase and high pressure liquid  chromatography, and by polyacrylamide gel electrophoresis. Purification of fraction II was achieved by the following steps: extraction of nucleic acid with phenol, precipitation of the RNA with cetyltrimethylammonium bromide, fractionation of the RNA with lithium chloride and isopropanol, and finally gel electrophoresis. A procedure using reverse phase chromatography was developed to obtain 70-90% recovery of RNA from polyacrylamide gels. Purified PSTV fraction II RNA was digested with ribonuclease A and T and labelled with [gamma-32P[ATP using polynucleotide kinase. The labelled digests were separated by the electrophoresis-homochromatography procedures of Sanger. About 20 and 30 spots were obtained with ribonuclease A and T, respectively.", "contents": "Separation of potato spindle tuber viroid ribonucleic acid from Scopolia sinensis into three infectious forms and the purification and oligonucleotide pattern of fraction II RNA. Part IX. The existence of three infectious forms of potato spindle tuber viroid (PSTV) RNA from Scopolia sinensis was demonstrated by fractionation with high salt, by reverse phase and high pressure liquid  chromatography, and by polyacrylamide gel electrophoresis. Purification of fraction II was achieved by the following steps: extraction of nucleic acid with phenol, precipitation of the RNA with cetyltrimethylammonium bromide, fractionation of the RNA with lithium chloride and isopropanol, and finally gel electrophoresis. A procedure using reverse phase chromatography was developed to obtain 70-90% recovery of RNA from polyacrylamide gels. Purified PSTV fraction II RNA was digested with ribonuclease A and T and labelled with [gamma-32P[ATP using polynucleotide kinase. The labelled digests were separated by the electrophoresis-homochromatography procedures of Sanger. About 20 and 30 spots were obtained with ribonuclease A and T, respectively.", "PMID": 953847}
{"id": "pubmed23n0032_742", "title": "The endogenous biosynthesis of prostaglandins by brain tissue in vitro.", "content": "The capacity for biosynthesis of prostaglandin F2alpha (PGF2alpha) and prostaglandin E2 (PGE2) from endogenous precursors by brain tissue slices and homogenates was measured by a gas chromatography - mass fragmentography method using deuterated prostaglandins as internal standards.  Mean biosynthesis in rat cerebral cortex slices incubated for 60 min was 60.2 ng PGF2alpha and 17.4 ng PGE2 per 100 mg of tissue. The corresponding values for homogenates were 78.1 ng and 28.9 ng. Synthetic capacity of cat cerebral cortex was considerably greater but that of human tissue was smaller than that found in rat brain. Cat cerebellum in contrast to other regions synthesized more PGE2 than PGF2alpha. The time-course of prostaglandin formation in slices was linear for the initial 60 min. Catabolism in cerebral tissues was found to be very small. Prostaglandins formed or added to the incubation media distributed between tissue and medium in a manner indicating some specific binding as well as nonspecific solubilization in tissue lipids. Norepinephrine, 3,4-L-dihydroxyphenylalanine, dopamine, adrenochrome and apomorphine greatly stimulated PGF2alpha formation probably through a nonenzymatic reduction of endoperoxides. Norepinephrine added to homogenates appeared to stabilize the fatty acid cyclo-oxygenase preventing it from inactivation. Indomethacin and Ketoprofen were potent inhibitors of biosynthesis. Paracetamol was found to be a less potent synthetase inhibitor than aspirin. The biosynthetic capacity of brain tissue in vitro appears to be orders of magnitude more than that of normal brain in situ.", "contents": "The endogenous biosynthesis of prostaglandins by brain tissue in vitro. The capacity for biosynthesis of prostaglandin F2alpha (PGF2alpha) and prostaglandin E2 (PGE2) from endogenous precursors by brain tissue slices and homogenates was measured by a gas chromatography - mass fragmentography method using deuterated prostaglandins as internal standards.  Mean biosynthesis in rat cerebral cortex slices incubated for 60 min was 60.2 ng PGF2alpha and 17.4 ng PGE2 per 100 mg of tissue. The corresponding values for homogenates were 78.1 ng and 28.9 ng. Synthetic capacity of cat cerebral cortex was considerably greater but that of human tissue was smaller than that found in rat brain. Cat cerebellum in contrast to other regions synthesized more PGE2 than PGF2alpha. The time-course of prostaglandin formation in slices was linear for the initial 60 min. Catabolism in cerebral tissues was found to be very small. Prostaglandins formed or added to the incubation media distributed between tissue and medium in a manner indicating some specific binding as well as nonspecific solubilization in tissue lipids. Norepinephrine, 3,4-L-dihydroxyphenylalanine, dopamine, adrenochrome and apomorphine greatly stimulated PGF2alpha formation probably through a nonenzymatic reduction of endoperoxides. Norepinephrine added to homogenates appeared to stabilize the fatty acid cyclo-oxygenase preventing it from inactivation. Indomethacin and Ketoprofen were potent inhibitors of biosynthesis. Paracetamol was found to be a less potent synthetase inhibitor than aspirin. The biosynthetic capacity of brain tissue in vitro appears to be orders of magnitude more than that of normal brain in situ.", "PMID": 953848}
{"id": "pubmed23n0032_743", "title": "Covalent assembly of mouse immunoglobulin G subclasses in vitro: application of a theoretical model for interchain disulfide bond formation.", "content": "The pathways and kinetics of interchain disulfide bond formation have been determined in vitro for purified myeloma proteins representing the three major subclasses of mouse immunoglobulin G(IgG) using the reoxidation system described previously (Petersen, J.G.L. & Dorrington, K.J. (1974) J. Biol. Chem. 249, 5633-5641). Mixtures of oxidized and reduced glutathione were added to act as a disulfide interchange catalyst. The pathways of covalent assembly observed in vitro were qualitatively and quantitatively similar to those followed by the various subclasses in vivo. HH and HHL were the principle covalent intermediates seen with IgG1 (MOPC 31C) and IgG2a (MOPC 173 and clone 19). With IgG2b( MPC 11C), HL, HH and HHL were all prominant intermediates. The time courses of reoxidation were simulated using a theoretical model based on second-order reaction kinetics (Percy, J.R., Percy, M.E. & Dorrington, K.J. (1974) J. Biol. Chem. 250, 2398-2400). Two distinct phases were apparent in the reoxidation sequence. The first, which lasted for the initial 5-15 min, did not confirm to the theoretical model. The second phase could be accounted for by the model and represented the remainder of the covalent assembly process. The physico-chemical basis for this biphasic phenomenon was explored. Sedimentation velocity studies showed that noncovalent association was incomplete at the beginning of the reoxidation step for all proteins except IgG2b (MOPC 11C). No dissociation was apparent in the reduced and alkylated proteins at pH 5 in the absence of prior exposure to acid conditions. Thus, exposure to acid appears to affect the affinity between the subunits in the native proteins. Transfer of the proteins from pH 5 to pH 8.2 (the pH at which reoxidation proceeds) is accompanied by the generation of an absorption difference spectrum over an 8-10 min period. These data suggest that a pH-dependent conformational relaxation process may influence the early stages of reoxidation.", "contents": "Covalent assembly of mouse immunoglobulin G subclasses in vitro: application of a theoretical model for interchain disulfide bond formation. The pathways and kinetics of interchain disulfide bond formation have been determined in vitro for purified myeloma proteins representing the three major subclasses of mouse immunoglobulin G(IgG) using the reoxidation system described previously (Petersen, J.G.L. & Dorrington, K.J. (1974) J. Biol. Chem. 249, 5633-5641). Mixtures of oxidized and reduced glutathione were added to act as a disulfide interchange catalyst. The pathways of covalent assembly observed in vitro were qualitatively and quantitatively similar to those followed by the various subclasses in vivo. HH and HHL were the principle covalent intermediates seen with IgG1 (MOPC 31C) and IgG2a (MOPC 173 and clone 19). With IgG2b( MPC 11C), HL, HH and HHL were all prominant intermediates. The time courses of reoxidation were simulated using a theoretical model based on second-order reaction kinetics (Percy, J.R., Percy, M.E. & Dorrington, K.J. (1974) J. Biol. Chem. 250, 2398-2400). Two distinct phases were apparent in the reoxidation sequence. The first, which lasted for the initial 5-15 min, did not confirm to the theoretical model. The second phase could be accounted for by the model and represented the remainder of the covalent assembly process. The physico-chemical basis for this biphasic phenomenon was explored. Sedimentation velocity studies showed that noncovalent association was incomplete at the beginning of the reoxidation step for all proteins except IgG2b (MOPC 11C). No dissociation was apparent in the reduced and alkylated proteins at pH 5 in the absence of prior exposure to acid conditions. Thus, exposure to acid appears to affect the affinity between the subunits in the native proteins. Transfer of the proteins from pH 5 to pH 8.2 (the pH at which reoxidation proceeds) is accompanied by the generation of an absorption difference spectrum over an 8-10 min period. These data suggest that a pH-dependent conformational relaxation process may influence the early stages of reoxidation.", "PMID": 953849}
{"id": "pubmed23n0032_744", "title": "Assembly of three major subclasses of mouse immunoglobulin G: a theoretical model for covalent assembly in vivo.", "content": "A mathematical model, based on second-order reaction kinetics, has been used to describe the covalent assembly of immunoglobulin G(IgG) in vitro from its heavy (H) and light (L) chains (Percy, M.E., Baumal, R., Dorrington, K.J. & Percy, J. (1976) Can. J. Biochem. 54, 675-687). In the present paper, the same model has now been applied to the steady-state assembly of IgG in vivo. This mathematical approach permits a quantitative comparison of the pathways of covalent assembly used by given immunoglobulins in vivo and in vitro. The assumptions in the model are: the species L, H, HL, HH, HHL and LHHL belong to a common pool; incompleted IgG intermediates may freely assemble to form HL, HH, HHL and LHHL; the reaction rate for covalent linkage between any two reacting species is proportional to the products of the number densities of the reactants and to a parameter P which takes the value PHH if the reaction joins two H chains, and PHL if it joins an H and L chain. In vivo values of PHH/PHL were determined for the 18 mouse myeloma tumours and cell lines studied by Baumal et al. (Baumal, R., Potter, M. & Scharff, M. (1971) J. Exp. Med. 134, 1316-1334). From these analyses, we have arrived at the following conclusions: (1) the three major IgG subclasses have distinctive values of PHH/PHL (mean value 53 for IgG1, 12 for IgG2a and 2.8 for IgG2b); (2) for IgGs of the same subclass, the values of PHH/PHL are similar; (3) the mean in vivo values of PHH/PHL are very close to those determined from in vitro assembly experiments. Finally, the individual values of PHH/PHL have been used to simulate pulse-chase experiments in the various tumours and cell lines. Considering the sources and magnitude of experimental error, the theoretical pathways of assembly agree with those determined qualitatively from the pulse-chase experiments.", "contents": "Assembly of three major subclasses of mouse immunoglobulin G: a theoretical model for covalent assembly in vivo. A mathematical model, based on second-order reaction kinetics, has been used to describe the covalent assembly of immunoglobulin G(IgG) in vitro from its heavy (H) and light (L) chains (Percy, M.E., Baumal, R., Dorrington, K.J. & Percy, J. (1976) Can. J. Biochem. 54, 675-687). In the present paper, the same model has now been applied to the steady-state assembly of IgG in vivo. This mathematical approach permits a quantitative comparison of the pathways of covalent assembly used by given immunoglobulins in vivo and in vitro. The assumptions in the model are: the species L, H, HL, HH, HHL and LHHL belong to a common pool; incompleted IgG intermediates may freely assemble to form HL, HH, HHL and LHHL; the reaction rate for covalent linkage between any two reacting species is proportional to the products of the number densities of the reactants and to a parameter P which takes the value PHH if the reaction joins two H chains, and PHL if it joins an H and L chain. In vivo values of PHH/PHL were determined for the 18 mouse myeloma tumours and cell lines studied by Baumal et al. (Baumal, R., Potter, M. & Scharff, M. (1971) J. Exp. Med. 134, 1316-1334). From these analyses, we have arrived at the following conclusions: (1) the three major IgG subclasses have distinctive values of PHH/PHL (mean value 53 for IgG1, 12 for IgG2a and 2.8 for IgG2b); (2) for IgGs of the same subclass, the values of PHH/PHL are similar; (3) the mean in vivo values of PHH/PHL are very close to those determined from in vitro assembly experiments. Finally, the individual values of PHH/PHL have been used to simulate pulse-chase experiments in the various tumours and cell lines. Considering the sources and magnitude of experimental error, the theoretical pathways of assembly agree with those determined qualitatively from the pulse-chase experiments.", "PMID": 953850}
{"id": "pubmed23n0032_745", "title": "The binding of phytohemagglutinin M to rat spleen lymphocytes. Quantitative studies.", "content": "Phytohemagglutinin M (PHAM) has been purified from the commercial mixture of proteins produced by Phaseolus vulgaris, using a Sepharose-thyroglobulin column. The protein gave one band on gel electrophoresis and two bands on SDS-gel electrophoresis (mol. wt. 33 700 and 32 100, respectively). Molecular weight determination by ultracentrifugation gave a value of 61 200 +/- 700. The protein had a minimum sugar content of 16%. Binding studies of PHAM to purified rat spleen lymphocytes have been performed at 0, 25, and 37 degrees C. It was shown that the cells bound about the same amount of lectin at 0 and 37 degrees C, but less protein was bound at 25 degrees C. The binding phenomenon showed saturability at all temperatures. Data were analyzed by Scatchard plots and two kinds of binding sites were found. High-affinity sites and low-affinity sites have been characterized in terms of association constants and (apparent) number. It was also shown that cells treated with trypsin or sodium azide bound less lectin. Bound concanavalin A did not appear to affect the amount of bound PHAM, but its influence was reflected in the value of the association constant for the binding of PHAM. Unlabelled PHAM was shown to displace radioactive PHAM from the cells, but could not remove bound concanavalin A. The significance of these results is discussed in terms of the fluid plasma membrane model and cellular metabolism.", "contents": "The binding of phytohemagglutinin M to rat spleen lymphocytes. Quantitative studies. Phytohemagglutinin M (PHAM) has been purified from the commercial mixture of proteins produced by Phaseolus vulgaris, using a Sepharose-thyroglobulin column. The protein gave one band on gel electrophoresis and two bands on SDS-gel electrophoresis (mol. wt. 33 700 and 32 100, respectively). Molecular weight determination by ultracentrifugation gave a value of 61 200 +/- 700. The protein had a minimum sugar content of 16%. Binding studies of PHAM to purified rat spleen lymphocytes have been performed at 0, 25, and 37 degrees C. It was shown that the cells bound about the same amount of lectin at 0 and 37 degrees C, but less protein was bound at 25 degrees C. The binding phenomenon showed saturability at all temperatures. Data were analyzed by Scatchard plots and two kinds of binding sites were found. High-affinity sites and low-affinity sites have been characterized in terms of association constants and (apparent) number. It was also shown that cells treated with trypsin or sodium azide bound less lectin. Bound concanavalin A did not appear to affect the amount of bound PHAM, but its influence was reflected in the value of the association constant for the binding of PHAM. Unlabelled PHAM was shown to displace radioactive PHAM from the cells, but could not remove bound concanavalin A. The significance of these results is discussed in terms of the fluid plasma membrane model and cellular metabolism.", "PMID": 953851}
{"id": "pubmed23n0032_746", "title": "Differential effects of pyridoxal 5'-phosphate on lysine residues in rabbit and sturgeon muscle aldolases.", "content": "Pyridoxal 5'-phosphate (PLP) in aqueous solutions can form a Schiff base complex with 14 and 16 lysine residues of rabbit and sturgeon muscle aldolases (EC 4.1.2.13), respectively. Although the mechanism of their interaction with PLP should be the same, these residues can be differentiated into three families on the basis of their inhibition constant Ki and rate constant k. The lysine residues of one of these families do not react with PLP in the presence of the substrates. Therefore, they are assumed to be part of the active center. In the sturgeon muscle aldolase, 3.7 substrate protected lysine residues are present. Rabbit aldolase, although tetrameric, contains only 2.8 substrate protected lysine residues. This suggests that one active center of this enzyme may be 'buried'. Structural studies showed the following sequence around the substrate protected lysine residues, in the rabbit aldolase: Gly-(Gly2, Val3)-Pyridoxyl Lys-Ile-Asp-Lys.", "contents": "Differential effects of pyridoxal 5'-phosphate on lysine residues in rabbit and sturgeon muscle aldolases. Pyridoxal 5'-phosphate (PLP) in aqueous solutions can form a Schiff base complex with 14 and 16 lysine residues of rabbit and sturgeon muscle aldolases (EC 4.1.2.13), respectively. Although the mechanism of their interaction with PLP should be the same, these residues can be differentiated into three families on the basis of their inhibition constant Ki and rate constant k. The lysine residues of one of these families do not react with PLP in the presence of the substrates. Therefore, they are assumed to be part of the active center. In the sturgeon muscle aldolase, 3.7 substrate protected lysine residues are present. Rabbit aldolase, although tetrameric, contains only 2.8 substrate protected lysine residues. This suggests that one active center of this enzyme may be 'buried'. Structural studies showed the following sequence around the substrate protected lysine residues, in the rabbit aldolase: Gly-(Gly2, Val3)-Pyridoxyl Lys-Ile-Asp-Lys.", "PMID": 953852}
{"id": "pubmed23n0032_747", "title": "Characterization of angiotensin receptors in rabbit isolated atria.", "content": "Rabbit isolated left atria have been used to study the inotropic action of angiotensin II (ATII). The peptide is active at doses ranging from 1.0 x 10(-9) to 2.8 x 10(-6)M and its inotropic effect is not modified by sotalol, phentolamine, burimamide, and indomethacin. We therefore propose that this effect results from the stimulation of receptors specific for ATII. Dose-response curves of ATII obtained in presence of increasing concentrations of 8-Gly-ATII are gradually displaced to the right, but high doses of the antagonist depressed the maximum contractions caused by ATII. pA2 value for 8-Gly-ATII in this preparation is similar to those observed in vascular and intestinal smooth muscles. Order of potency of analogues of ATII (2-, 3- and 5-Ala-ATII), acting as full agonists, but with reduced affinity, is similar to that found in rabbit aorta strips. It is therefore proposed that receptors for ATII in rabbit isolated left atria are pharmacologically similar to those present in vascular smooth muscles. Positive inotropic effects of angiotensin I, undecapeptide (1-11), dodecapeptide (1-12) and tetradecapeptide (1-14) renin substrate are antagonized by 8-Gly-ATII in similar way as the effect of ATII. This suggests that the action of these peptides is mainly due to stimulation of receptors for ATII. The contribution of myocardial converting enzyme to the action of these peptides is discussed.", "contents": "Characterization of angiotensin receptors in rabbit isolated atria. Rabbit isolated left atria have been used to study the inotropic action of angiotensin II (ATII). The peptide is active at doses ranging from 1.0 x 10(-9) to 2.8 x 10(-6)M and its inotropic effect is not modified by sotalol, phentolamine, burimamide, and indomethacin. We therefore propose that this effect results from the stimulation of receptors specific for ATII. Dose-response curves of ATII obtained in presence of increasing concentrations of 8-Gly-ATII are gradually displaced to the right, but high doses of the antagonist depressed the maximum contractions caused by ATII. pA2 value for 8-Gly-ATII in this preparation is similar to those observed in vascular and intestinal smooth muscles. Order of potency of analogues of ATII (2-, 3- and 5-Ala-ATII), acting as full agonists, but with reduced affinity, is similar to that found in rabbit aorta strips. It is therefore proposed that receptors for ATII in rabbit isolated left atria are pharmacologically similar to those present in vascular smooth muscles. Positive inotropic effects of angiotensin I, undecapeptide (1-11), dodecapeptide (1-12) and tetradecapeptide (1-14) renin substrate are antagonized by 8-Gly-ATII in similar way as the effect of ATII. This suggests that the action of these peptides is mainly due to stimulation of receptors for ATII. The contribution of myocardial converting enzyme to the action of these peptides is discussed.", "PMID": 953853}
{"id": "pubmed23n0032_748", "title": "Application of a multiwire proportional chamber to the detection of axoplasmic transport.", "content": "A new technique for the detection of the axoplasmic transport of beta-radioactively labelled materials is described wherein a multiwire proportional chamber is used to measure the distribution of activity along peripheral nerve fibers which are maintained in vitro. The operating principles of the chamber are described and basic construction parameters given. Potential radiolabels are discussed. Two types of studies were performed at room temperature in vitro using sciatic nerves of the amphibian Xenopus laevis: static and dynamic. In the static study the nerve ganglion was incubated for a suitable period of time in either L-(U-14C) leucine or L-(35S) methionine after which the ganglion was removed and the activity in the remaining nerve assayed with the chamber. In the dynamic study the nerve activity was assayed by the chamber while incubation proceeded so that a dynamic picture of transport could be observed. Using the second approach, transport rates were observed which are in agreement with others which have been reported in the literature. Some advantages and limitations of the technique are discussed.", "contents": "Application of a multiwire proportional chamber to the detection of axoplasmic transport. A new technique for the detection of the axoplasmic transport of beta-radioactively labelled materials is described wherein a multiwire proportional chamber is used to measure the distribution of activity along peripheral nerve fibers which are maintained in vitro. The operating principles of the chamber are described and basic construction parameters given. Potential radiolabels are discussed. Two types of studies were performed at room temperature in vitro using sciatic nerves of the amphibian Xenopus laevis: static and dynamic. In the static study the nerve ganglion was incubated for a suitable period of time in either L-(U-14C) leucine or L-(35S) methionine after which the ganglion was removed and the activity in the remaining nerve assayed with the chamber. In the dynamic study the nerve activity was assayed by the chamber while incubation proceeded so that a dynamic picture of transport could be observed. Using the second approach, transport rates were observed which are in agreement with others which have been reported in the literature. Some advantages and limitations of the technique are discussed.", "PMID": 953854}
{"id": "pubmed23n0032_749", "title": "Anti-inflammatory and analgesic properties of etodolic acid in rats.", "content": "Etodolic acid (1, 8-diethyl-1, 3, 4, 9-tetrahydropyrano(3, 4-b) indole-1-acetic acid) showed potent anti-inflammatory and analgesic properties in rats. In adjuvant arthritic rats etodolic acid was approximately six times more potent than phenylbutazone. In the carrageenin and the analgesic assays (inflamed paw pressure test) its potency was comparable to that of phenylbutazone. The potency of etodolic acid in the adjuvant arthritic rat suggests that this novel anti-inflammatory compound will be effective in the treatment of arthritic conditions of man.", "contents": "Anti-inflammatory and analgesic properties of etodolic acid in rats. Etodolic acid (1, 8-diethyl-1, 3, 4, 9-tetrahydropyrano(3, 4-b) indole-1-acetic acid) showed potent anti-inflammatory and analgesic properties in rats. In adjuvant arthritic rats etodolic acid was approximately six times more potent than phenylbutazone. In the carrageenin and the analgesic assays (inflamed paw pressure test) its potency was comparable to that of phenylbutazone. The potency of etodolic acid in the adjuvant arthritic rat suggests that this novel anti-inflammatory compound will be effective in the treatment of arthritic conditions of man.", "PMID": 953855}
{"id": "pubmed23n0032_750", "title": "A specific sugar transport mechanism in smooth muscle and its regulation.", "content": "The membrane transport of 3-O-methyl-D-glucose was studied in vitro in a smooth muscle, the detrusor of rat urinary bladder. Transport occurred by facilitated diffusion and showed the same chemical specificity and sensitivity to specific inhibitors as skeletal and cardiac muscle but its insulin sensitivity was smaller. Transport was increased by agents inhibiting the Na+pump and was decreased by agents which increased Na+ and K+ gradients by apparently stimulating the Na+pump. In accord with a rate limiting role of transport in glucose utilization, similar stimulating and inhibitory effects were seen when CO2 production from (14C) glucose was measured.", "contents": "A specific sugar transport mechanism in smooth muscle and its regulation. The membrane transport of 3-O-methyl-D-glucose was studied in vitro in a smooth muscle, the detrusor of rat urinary bladder. Transport occurred by facilitated diffusion and showed the same chemical specificity and sensitivity to specific inhibitors as skeletal and cardiac muscle but its insulin sensitivity was smaller. Transport was increased by agents inhibiting the Na+pump and was decreased by agents which increased Na+ and K+ gradients by apparently stimulating the Na+pump. In accord with a rate limiting role of transport in glucose utilization, similar stimulating and inhibitory effects were seen when CO2 production from (14C) glucose was measured.", "PMID": 953856}
{"id": "pubmed23n0032_751", "title": "[A method to measure the rate of metabolic clearance and secretion of cortisol in the eel (Anguilla anguilla L.)].", "content": "Classical methods for the analysis of steroid dynamics were adapted to measure the metabolic clearance rate and the secretion rate of cortisol in the European eel using (1, 2-3H) cortisol as a tracer. 1. Measurements were made whilst the eels were in the steady state; stresses which usually result from anaesthesia and handling were avoided by chronic catheterization of the blood vessels of the swim bladder. In this way, the plasma cortisol level were constant; thus, it was possible to obtain valid measurements of the cortisol secretion rate knowing the metabolic clearance rate and the plasma cortisol level. 2. In the eel, cortisol and cortisone together form a chemical compartment', derived originally from cortisol secreted by the interrenal gland and subsequently transformed partially into cortisone in the plasma and maintained thenceforth in dynamic reversible equilibrium. Consequently, the metabolic clearance rate of cortisol has been calculated from the known changes in concentration of both (1, 2-3H) cortisol and (1, 2-3H)cortisone in the plasma deduced from dichloromethane-extractable plasma radioactivity, 72% of which is always attributable to these two steroids from the moment that the radioactivity begins to decline regularly as a function of time (15-30 min after the injection). 3. After a single injection of (1,2-3H) cortisol, the interpretation of the disappearance curve by compartmental analysis is complicated by the delay of tracer distribution and by the evident variability in the initial portion of the curve. Disappearance curves have accordingly been analysed in a more rigorous fashion either by integrating the area under each curve which allows one to calculate individually the metabolic clearance rates, or by integrating according to Normand, M., and Fortier, C. (1970. Can. J. Physiol. Pharmacol. 48, 274-281) which allows one statistical treatment of data. 4. Estimates of the metabolic clearance rate of cortisol were made in a satisfactory manner by either one of the two integration methods. They were also very similar to estimates obtained by prolonged infusion of (1, 2-3H) cortisol to constant specific activity into eels maintained under identical experimental conditions.", "contents": "[A method to measure the rate of metabolic clearance and secretion of cortisol in the eel (Anguilla anguilla L.)]. Classical methods for the analysis of steroid dynamics were adapted to measure the metabolic clearance rate and the secretion rate of cortisol in the European eel using (1, 2-3H) cortisol as a tracer. 1. Measurements were made whilst the eels were in the steady state; stresses which usually result from anaesthesia and handling were avoided by chronic catheterization of the blood vessels of the swim bladder. In this way, the plasma cortisol level were constant; thus, it was possible to obtain valid measurements of the cortisol secretion rate knowing the metabolic clearance rate and the plasma cortisol level. 2. In the eel, cortisol and cortisone together form a chemical compartment', derived originally from cortisol secreted by the interrenal gland and subsequently transformed partially into cortisone in the plasma and maintained thenceforth in dynamic reversible equilibrium. Consequently, the metabolic clearance rate of cortisol has been calculated from the known changes in concentration of both (1, 2-3H) cortisol and (1, 2-3H)cortisone in the plasma deduced from dichloromethane-extractable plasma radioactivity, 72% of which is always attributable to these two steroids from the moment that the radioactivity begins to decline regularly as a function of time (15-30 min after the injection). 3. After a single injection of (1,2-3H) cortisol, the interpretation of the disappearance curve by compartmental analysis is complicated by the delay of tracer distribution and by the evident variability in the initial portion of the curve. Disappearance curves have accordingly been analysed in a more rigorous fashion either by integrating the area under each curve which allows one to calculate individually the metabolic clearance rates, or by integrating according to Normand, M., and Fortier, C. (1970. Can. J. Physiol. Pharmacol. 48, 274-281) which allows one statistical treatment of data. 4. Estimates of the metabolic clearance rate of cortisol were made in a satisfactory manner by either one of the two integration methods. They were also very similar to estimates obtained by prolonged infusion of (1, 2-3H) cortisol to constant specific activity into eels maintained under identical experimental conditions.", "PMID": 953857}
{"id": "pubmed23n0032_752", "title": "A compartmental model for bilirubin kinetics in isolated perfused rat liver.", "content": "Bilirubin kinetics were studied in an isolated, perfused rat liver system using unconjugated (14C) bilirubin (UC(14C)B) and  delta-amino (4-14 C) levulinic acid (A(14 C)LA) to derive a suitable compartmental model. Plasma disappearance of UC(14C)B, plasma appearance of conjugated (14c) bilirubin (C(14C)B) and biliary excretion of C(14C)B were followed for 90-120 min following injection of UC(14C)B. Hepatic content of labeled bilirubin 12 min after the injection  of UC(14C)B was determined directly in five separate perfusion experiments. UCB was found to reflux back to plasma from liver in two experiments using A(14C)LA. Bilirubin binding to red blood cells (6-8% of the perfusate level) and the components of the perfusion apparatus (4-6% of perfusate level) was estimated by performing a control experiment without the liver. A six compartment model was necessary and adequate to explain the experimental data and current knowledge of bilirubin metabolism: (1) UCB bound to red blood cells and the perfusion apparatus, (2) plasma UCB, (3) liver UCB, (4) liver CB, (5) plasma CB, and (6) bile CB. The proposed model could serve as a reference point for studies of bilirubin kinetics in whole animals for normal and abnormal states.", "contents": "A compartmental model for bilirubin kinetics in isolated perfused rat liver. Bilirubin kinetics were studied in an isolated, perfused rat liver system using unconjugated (14C) bilirubin (UC(14C)B) and  delta-amino (4-14 C) levulinic acid (A(14 C)LA) to derive a suitable compartmental model. Plasma disappearance of UC(14C)B, plasma appearance of conjugated (14c) bilirubin (C(14C)B) and biliary excretion of C(14C)B were followed for 90-120 min following injection of UC(14C)B. Hepatic content of labeled bilirubin 12 min after the injection  of UC(14C)B was determined directly in five separate perfusion experiments. UCB was found to reflux back to plasma from liver in two experiments using A(14C)LA. Bilirubin binding to red blood cells (6-8% of the perfusate level) and the components of the perfusion apparatus (4-6% of perfusate level) was estimated by performing a control experiment without the liver. A six compartment model was necessary and adequate to explain the experimental data and current knowledge of bilirubin metabolism: (1) UCB bound to red blood cells and the perfusion apparatus, (2) plasma UCB, (3) liver UCB, (4) liver CB, (5) plasma CB, and (6) bile CB. The proposed model could serve as a reference point for studies of bilirubin kinetics in whole animals for normal and abnormal states.", "PMID": 953858}
{"id": "pubmed23n0032_753", "title": "Adrenergic neuron blocking action of dehydrocorydaline isolated from Corydalis bulbosa.", "content": "Dehydrocorydaline, an active principle of Corydalis bulbosa alkaloids, in concentrations of 10(-5) M to 5 x 10(-5)M inhibited relaxation and the concomitant release of (3H)-noradrenaline caused by 10(-4)M nicotine and electrical perivascular nerve stimulation in the taenia caecum of guinea pig. The same inhibitory effects were observed on contraction and release of (3H) noradrenaline in the sympathetic nerve-pulmonary artery preparation of rabbit. On the other hand, neither relaxation nor contraction caused by exogenously applied noradrenaline was affected. These results suggest that the inhibitory action of dehydrocorydaline on the relaxation or contraction, produced by nicotine and electrical nerve stimulation, is due to blockade of noradrenaline release from the adrenergic nerve terminals in both the taenia caecum and pulmonary artery. Participation of the adrenergic neuron blocking action of dehydrocorydaline in preventing experimental ulceration is discussed.", "contents": "Adrenergic neuron blocking action of dehydrocorydaline isolated from Corydalis bulbosa. Dehydrocorydaline, an active principle of Corydalis bulbosa alkaloids, in concentrations of 10(-5) M to 5 x 10(-5)M inhibited relaxation and the concomitant release of (3H)-noradrenaline caused by 10(-4)M nicotine and electrical perivascular nerve stimulation in the taenia caecum of guinea pig. The same inhibitory effects were observed on contraction and release of (3H) noradrenaline in the sympathetic nerve-pulmonary artery preparation of rabbit. On the other hand, neither relaxation nor contraction caused by exogenously applied noradrenaline was affected. These results suggest that the inhibitory action of dehydrocorydaline on the relaxation or contraction, produced by nicotine and electrical nerve stimulation, is due to blockade of noradrenaline release from the adrenergic nerve terminals in both the taenia caecum and pulmonary artery. Participation of the adrenergic neuron blocking action of dehydrocorydaline in preventing experimental ulceration is discussed.", "PMID": 953859}
{"id": "pubmed23n0032_754", "title": "Motor and sensory fibres of neck muscle nerves in the cat.", "content": "The numbers and sizes of nerve fibres to the dorsal neck muscles, splenius, complexus and biventer cervicis have been examined in the cat. The total number of fibres is unusually high as is the content of sensory fibres (estimated as the loss of fibres after ganglionectomy). The fibre spectra of these sensory nerves has an unusually large number of fibres in the group II and III range (3-7 mum) and differs markedly in this way from other muscle nerves. The motor fibres contain a high proportion (64-99%) in the gamma fibre size range. Large motor fibres are absent in the nerves to biventer cervicis (a slow muscle). The ratio of unmyelinated to myelinated fibres in neck muscle nerves is similar to that in hind legs at about 2.5:1.", "contents": "Motor and sensory fibres of neck muscle nerves in the cat. The numbers and sizes of nerve fibres to the dorsal neck muscles, splenius, complexus and biventer cervicis have been examined in the cat. The total number of fibres is unusually high as is the content of sensory fibres (estimated as the loss of fibres after ganglionectomy). The fibre spectra of these sensory nerves has an unusually large number of fibres in the group II and III range (3-7 mum) and differs markedly in this way from other muscle nerves. The motor fibres contain a high proportion (64-99%) in the gamma fibre size range. Large motor fibres are absent in the nerves to biventer cervicis (a slow muscle). The ratio of unmyelinated to myelinated fibres in neck muscle nerves is similar to that in hind legs at about 2.5:1.", "PMID": 953860}
{"id": "pubmed23n0032_755", "title": "Further evidence that protein synthesis can be decreased in vivo following hormonal stimulation in the rat pancreas.", "content": "The present study has been undertaken to determine in the rat the influence of exocrine secretory stimulation on pancreatic protein synthesis. This stimulant consisted of a single injection of cholecystokinin-pancreozymin (8 Ivy units/kg) plus secretin (5 clinical units/kg). The rate of (14C) phenylalanine incorporation into total proteins was measured 5, 11, 17, 30, 45 and 60 min later. Incorporation was significantly decreased after 5 min, then significantly increased at 17 min, and finally returned to control values at 45 min. This biphasic evolution was shown not to be caused by variations in the precursor pool specific radioactivity. We concluded that secretory stimulation of the pancreas can induce a decrease in the rate of protein biosynthesis. This decrease is nevertheless a transient phenomenon, since the rate of biosynthesis was increased at 17 min. These results, obtained from a totally in vivo system, confirm previous data obtained from an in vivo-in vitro system.", "contents": "Further evidence that protein synthesis can be decreased in vivo following hormonal stimulation in the rat pancreas. The present study has been undertaken to determine in the rat the influence of exocrine secretory stimulation on pancreatic protein synthesis. This stimulant consisted of a single injection of cholecystokinin-pancreozymin (8 Ivy units/kg) plus secretin (5 clinical units/kg). The rate of (14C) phenylalanine incorporation into total proteins was measured 5, 11, 17, 30, 45 and 60 min later. Incorporation was significantly decreased after 5 min, then significantly increased at 17 min, and finally returned to control values at 45 min. This biphasic evolution was shown not to be caused by variations in the precursor pool specific radioactivity. We concluded that secretory stimulation of the pancreas can induce a decrease in the rate of protein biosynthesis. This decrease is nevertheless a transient phenomenon, since the rate of biosynthesis was increased at 17 min. These results, obtained from a totally in vivo system, confirm previous data obtained from an in vivo-in vitro system.", "PMID": 953861}
{"id": "pubmed23n0032_756", "title": "Potassium and the recovery of splenic capsular smooth muscle after cold storage.", "content": "Cat spleen capsular smooth muscle, depleted of potassium and enriched with sodium by cold storage in a potassium free medium, relaxed and underwent transient reduction in responsiveness to noradrenaline when potassium was introduced into the bathing medium. Both these effects could be blocked by ouabain, lithium substitution for sodium or low ambient temperature, suggesting possible involvement of the sodium pump. In the continued presence of potassium, relaxation was maintained but sensitivity to noradrenaline increased, possibly due to restoration of normal intracellular sodium and potassium concentrations.", "contents": "Potassium and the recovery of splenic capsular smooth muscle after cold storage. Cat spleen capsular smooth muscle, depleted of potassium and enriched with sodium by cold storage in a potassium free medium, relaxed and underwent transient reduction in responsiveness to noradrenaline when potassium was introduced into the bathing medium. Both these effects could be blocked by ouabain, lithium substitution for sodium or low ambient temperature, suggesting possible involvement of the sodium pump. In the continued presence of potassium, relaxation was maintained but sensitivity to noradrenaline increased, possibly due to restoration of normal intracellular sodium and potassium concentrations.", "PMID": 953862}
{"id": "pubmed23n0032_757", "title": "The effects of selenium on the metabolism of methionine in sheep.", "content": "Two groups of sheep fed a diet of hay known to produce nutritional muscular dystrophy, one group of which received selenium supplementation, were used to study the effects of selenium on the metabolism of administered L-(35S) methionine by rumen microflora. Rumen bacterial proteins of the Se supplemented sheep contained significantly higher levels of radiosulfur than the bacterial protein of the non-supplemented sheep. Of hte L-(35S) methionine present in the rumen liquor samples from Se-supplemented sheep 2 h after administration, 13.3% of the amino acid, which was measured as methionine sulfone, was found in the microbial proteins. A large proportion of the administered labeled methionine was resynthesized as cyst(e)ine which may account in part for that determined as cysteic acid in rumen bacterial and plasma proteins. The observed low levels of radiosulfur found in rumen microflora from selenium deficient wethers, indicates that the presence of selenium profoundly affects the rate of methionine metabolism and the distribution of methionine in rumen bacterial and protozoal proteins. In another experiment, the effect of selenium on the metabolism of L-(Me-3H) methionine was studied. The selenium status of the sheep had no significant effect (P greater than 0.05) on the distribution of 35S radioactivity in the blood plasma and tissues.", "contents": "The effects of selenium on the metabolism of methionine in sheep. Two groups of sheep fed a diet of hay known to produce nutritional muscular dystrophy, one group of which received selenium supplementation, were used to study the effects of selenium on the metabolism of administered L-(35S) methionine by rumen microflora. Rumen bacterial proteins of the Se supplemented sheep contained significantly higher levels of radiosulfur than the bacterial protein of the non-supplemented sheep. Of hte L-(35S) methionine present in the rumen liquor samples from Se-supplemented sheep 2 h after administration, 13.3% of the amino acid, which was measured as methionine sulfone, was found in the microbial proteins. A large proportion of the administered labeled methionine was resynthesized as cyst(e)ine which may account in part for that determined as cysteic acid in rumen bacterial and plasma proteins. The observed low levels of radiosulfur found in rumen microflora from selenium deficient wethers, indicates that the presence of selenium profoundly affects the rate of methionine metabolism and the distribution of methionine in rumen bacterial and protozoal proteins. In another experiment, the effect of selenium on the metabolism of L-(Me-3H) methionine was studied. The selenium status of the sheep had no significant effect (P greater than 0.05) on the distribution of 35S radioactivity in the blood plasma and tissues.", "PMID": 953863}
{"id": "pubmed23n0032_758", "title": "Vascular actions of furosemide and bumetanide on the rat superior mesenteric vascular bed: interactions with prolactin and prostaglandins.", "content": "The mesenteric vascular bed of the rat was used to investigate the effects of furosemide, bumetanide, prolactin, aspirin, indomethacin and prostaglandin E2 on the pressor response to norepinephrine. Furosemide, bumetanied and indomethacin could all inhibit the responses to norepinephrine: in each case responsiveness was restored by the addition of prostaglandin E2 to the perfusate. Bumetanide and furosemide both failed to inhibit responsiveness in the presence of an adequate amount (50 pg/ml) of prostaglandin E2. As has been shown previously, ovine prolactin in a concentration of 50 ng/ml enhanced pressor responses to norepinephrine while 500 ng/ml, after an initial potentiation, inhibited responsiveness. Aspirin, furosemide and bumetanide all reversed both the potentiation produced by the lower prolactin concentration and the inhibition produced by the higher one. When taken in conjunction with other evidence these results suggest that the diuretics exert their vascular effects by inhibiting prostaglandin synthesis whereas prolactin acts by stimulating such synthesis.", "contents": "Vascular actions of furosemide and bumetanide on the rat superior mesenteric vascular bed: interactions with prolactin and prostaglandins. The mesenteric vascular bed of the rat was used to investigate the effects of furosemide, bumetanide, prolactin, aspirin, indomethacin and prostaglandin E2 on the pressor response to norepinephrine. Furosemide, bumetanied and indomethacin could all inhibit the responses to norepinephrine: in each case responsiveness was restored by the addition of prostaglandin E2 to the perfusate. Bumetanide and furosemide both failed to inhibit responsiveness in the presence of an adequate amount (50 pg/ml) of prostaglandin E2. As has been shown previously, ovine prolactin in a concentration of 50 ng/ml enhanced pressor responses to norepinephrine while 500 ng/ml, after an initial potentiation, inhibited responsiveness. Aspirin, furosemide and bumetanide all reversed both the potentiation produced by the lower prolactin concentration and the inhibition produced by the higher one. When taken in conjunction with other evidence these results suggest that the diuretics exert their vascular effects by inhibiting prostaglandin synthesis whereas prolactin acts by stimulating such synthesis.", "PMID": 953864}
{"id": "pubmed23n0032_759", "title": "A method for the study of the inhibition of DNA synthesis by rabbit tissue extracts.", "content": "This paper describes a method to measure DNA synthesis in the jejunum. Everted rings of rat jejunum were incubated in the presence of (3H) thymidine. The specific activity of jejunal DNA was assayed at the end of incubation. This method was found to be valid when (1) incubation time was 30 min, (2) (3H) thymidine concentration was 90 ng/ml, and (3) paired flasks containing four, respectively, contiguous rings were compared. This method was used to investigate the existence of an intestinal chalone. An aqueous extract (S-105)of rabbit small intestine mucosa was prepared and tested for DNA synthesis inhibition in jejunum. A significant inhibition was noted, which was an hyperbolic function of the quantity of extract present in the media. The study of S-105 inhibition on other organs (kidney, liver, colon) failed to demonstrate organ specificity. However, aqueous extracts from intestinal organs were found to exert a greater inhibition than aqueous extracts form other organs. It is suggested that S-105 contains other nonspecific inhibitory fractions; our data prove the necessity of an extensive purification to demonstrate the existence of a specific chalone that regulates intestinal cell proliferation.", "contents": "A method for the study of the inhibition of DNA synthesis by rabbit tissue extracts. This paper describes a method to measure DNA synthesis in the jejunum. Everted rings of rat jejunum were incubated in the presence of (3H) thymidine. The specific activity of jejunal DNA was assayed at the end of incubation. This method was found to be valid when (1) incubation time was 30 min, (2) (3H) thymidine concentration was 90 ng/ml, and (3) paired flasks containing four, respectively, contiguous rings were compared. This method was used to investigate the existence of an intestinal chalone. An aqueous extract (S-105)of rabbit small intestine mucosa was prepared and tested for DNA synthesis inhibition in jejunum. A significant inhibition was noted, which was an hyperbolic function of the quantity of extract present in the media. The study of S-105 inhibition on other organs (kidney, liver, colon) failed to demonstrate organ specificity. However, aqueous extracts from intestinal organs were found to exert a greater inhibition than aqueous extracts form other organs. It is suggested that S-105 contains other nonspecific inhibitory fractions; our data prove the necessity of an extensive purification to demonstrate the existence of a specific chalone that regulates intestinal cell proliferation.", "PMID": 953865}
{"id": "pubmed23n0032_760", "title": "Effect of (Sar1, Ala8)-angiotensin II and hypophysectomy on the intestinal resistance vessels and blood pressure following furosemide-induced volume depletion.", "content": "Intravenous administration of furosemide (2 mg/kg) caused intestinal vasoconstriction in various groups of pentobarbital-anesthetized cats. (Sar1, Ala 8)-angiotensin II, a specific competitive antagonist of angiotensin II, was infused 60 min after administration of furosemide, a time when the intestinal vasoconstrictor response to the diuretic was maximal or near maximal. In hypophysectomized animals, infusion of the antagonist abolished the intestinal vasoconstriction and caused a significant fall in arterial pressure even when the intestinal nerves and adrenal glands remained intact. In contrast, the antagonist had little effect when the pituitary gland remained intact. The results suggest that endogenous angiotensin and vasopressin are overlapping mechanisms which constrict the intestinal resistance vessels and support arterial pressure following furosemide-induced volume depletion. In the absence of one control system, the other compensates to maintain the responses.", "contents": "Effect of (Sar1, Ala8)-angiotensin II and hypophysectomy on the intestinal resistance vessels and blood pressure following furosemide-induced volume depletion. Intravenous administration of furosemide (2 mg/kg) caused intestinal vasoconstriction in various groups of pentobarbital-anesthetized cats. (Sar1, Ala 8)-angiotensin II, a specific competitive antagonist of angiotensin II, was infused 60 min after administration of furosemide, a time when the intestinal vasoconstrictor response to the diuretic was maximal or near maximal. In hypophysectomized animals, infusion of the antagonist abolished the intestinal vasoconstriction and caused a significant fall in arterial pressure even when the intestinal nerves and adrenal glands remained intact. In contrast, the antagonist had little effect when the pituitary gland remained intact. The results suggest that endogenous angiotensin and vasopressin are overlapping mechanisms which constrict the intestinal resistance vessels and support arterial pressure following furosemide-induced volume depletion. In the absence of one control system, the other compensates to maintain the responses.", "PMID": 953866}
{"id": "pubmed23n0032_761", "title": "Duration of analgesia in mice after heroin by two testing methods.", "content": "The analgestic action in mice of single injections of heroin hydrochloride ranging from 0.3-240mg/kg was measured by the tail-clip and by the hot-plate methods. The duration of analgesia increased as the dose of heroin increased. By the tail-clip technique, the mean effective dose (ED50) (and standard error of estimate) at 30 min was calculated as 1.0 (+/3.42) mg/kg while at 180 min it was 27.5 (+/3.05). By the hot-plate technique the ED50 at 30 min was 4.9 (+/3.13) mg/kg and at 180 min it was 173.8 (+/5.38) mg/kg. The hot-plate method, though less sensitive than the tail-clip method, yields a regression line derived from ED50 values at various testing times of the same slope; thus the two methods give comparable results for changes in analgesia with time. The rate of change of the median analgesic dose of heroin HC1 in mice was calculated to be 2% per minute. In similar mice the acute mean lethal dose for single, subcutaneous injections of heroin HC1 was calculated to be 190.5+/3.01 mg/kg (95% confidence limits).", "contents": "Duration of analgesia in mice after heroin by two testing methods. The analgestic action in mice of single injections of heroin hydrochloride ranging from 0.3-240mg/kg was measured by the tail-clip and by the hot-plate methods. The duration of analgesia increased as the dose of heroin increased. By the tail-clip technique, the mean effective dose (ED50) (and standard error of estimate) at 30 min was calculated as 1.0 (+/3.42) mg/kg while at 180 min it was 27.5 (+/3.05). By the hot-plate technique the ED50 at 30 min was 4.9 (+/3.13) mg/kg and at 180 min it was 173.8 (+/5.38) mg/kg. The hot-plate method, though less sensitive than the tail-clip method, yields a regression line derived from ED50 values at various testing times of the same slope; thus the two methods give comparable results for changes in analgesia with time. The rate of change of the median analgesic dose of heroin HC1 in mice was calculated to be 2% per minute. In similar mice the acute mean lethal dose for single, subcutaneous injections of heroin HC1 was calculated to be 190.5+/3.01 mg/kg (95% confidence limits).", "PMID": 953867}
{"id": "pubmed23n0032_762", "title": "The effect of metoclopramide on intestinal muscle responses and the peristaltic reflex in vitro.", "content": "Metoclopramide (Mcp) is known to facilitate gastrointestinal emptying in vivo and to stimulate various isolated intestinal muscle preparations. On the guinea pig ileum, taenia coli, rabbit ileum and rat duodenum, Mcp increased the tone and responses to acetylcholine, carbachol and nicotine; had no effect on responses to histamine, potassium chloride and prostaglandin E1; decreased responses to 5-hydroxytryptamine (5-HT). Atropine, methysergide, morphine, and tetrodotoxin, alone or in combination, partially blocked the stimulatory responses to Mcp, but hexamethonium, mepyramine and indomethacin did not. Mcp (1.0 muM) lowered the threshold for elicitation of the peristaltic reflex to a sub-threshold intraluminal pressure (2.5 cm water), facilitated the peristaltic response to threshold pressures (3-4 cm water) and restored the reflex in fatigued preparations, but not that depressed by cooling to 24 degrees C. During block of peristalsis by atropine, hexamethonium or methysergide (applied serosally) 5-HT (0.25 muM) but not Mcp (1.0 muM)) effectively restored the peristaltic reflex, but neither antagonized the inhibition by morphine or procaine acting serosally. However, Mcp (1.0 muM) re-established peristalsis inhibited by a high concentration of 5-HT ((4 X 10 muM). These results do not support the hypotheses that the stimulatory action of Mcp is entirely dependent on either peripheral sensitization of muscarinic receptors or an action on tryptaminergic mechanisms but are consistent with our previous conclusion that an additional component may be a blockade of some intrinsic inhibitory (possibly purinergic) substance normally restraining intestinal motility or tone.", "contents": "The effect of metoclopramide on intestinal muscle responses and the peristaltic reflex in vitro. Metoclopramide (Mcp) is known to facilitate gastrointestinal emptying in vivo and to stimulate various isolated intestinal muscle preparations. On the guinea pig ileum, taenia coli, rabbit ileum and rat duodenum, Mcp increased the tone and responses to acetylcholine, carbachol and nicotine; had no effect on responses to histamine, potassium chloride and prostaglandin E1; decreased responses to 5-hydroxytryptamine (5-HT). Atropine, methysergide, morphine, and tetrodotoxin, alone or in combination, partially blocked the stimulatory responses to Mcp, but hexamethonium, mepyramine and indomethacin did not. Mcp (1.0 muM) lowered the threshold for elicitation of the peristaltic reflex to a sub-threshold intraluminal pressure (2.5 cm water), facilitated the peristaltic response to threshold pressures (3-4 cm water) and restored the reflex in fatigued preparations, but not that depressed by cooling to 24 degrees C. During block of peristalsis by atropine, hexamethonium or methysergide (applied serosally) 5-HT (0.25 muM) but not Mcp (1.0 muM)) effectively restored the peristaltic reflex, but neither antagonized the inhibition by morphine or procaine acting serosally. However, Mcp (1.0 muM) re-established peristalsis inhibited by a high concentration of 5-HT ((4 X 10 muM). These results do not support the hypotheses that the stimulatory action of Mcp is entirely dependent on either peripheral sensitization of muscarinic receptors or an action on tryptaminergic mechanisms but are consistent with our previous conclusion that an additional component may be a blockade of some intrinsic inhibitory (possibly purinergic) substance normally restraining intestinal motility or tone.", "PMID": 953868}
{"id": "pubmed23n0032_763", "title": "Brown adipose tissue-a factor in the survival of harp seal pups.", "content": "The respiratory characteristics of mitochondria isolated from the subcutaneous brown adipose tissue of newborn harp seals indicate that the tissue is thermogenically active. Temperature recordings in vivo revealed, in fact, that the tissue was maintained at a temperature close to that of the body core during immersion of the pups in ice-water. Beta-adrenergic blockade markedly increased the cooling rates at both locations in ice-water, while curarization, accompanied by artificial respiration did not. We conclude that nonshivering thermogenesis through activated brown adipose tissue plays a decisive role in the defence against cold in the newborn harp seal.", "contents": "Brown adipose tissue-a factor in the survival of harp seal pups. The respiratory characteristics of mitochondria isolated from the subcutaneous brown adipose tissue of newborn harp seals indicate that the tissue is thermogenically active. Temperature recordings in vivo revealed, in fact, that the tissue was maintained at a temperature close to that of the body core during immersion of the pups in ice-water. Beta-adrenergic blockade markedly increased the cooling rates at both locations in ice-water, while curarization, accompanied by artificial respiration did not. We conclude that nonshivering thermogenesis through activated brown adipose tissue plays a decisive role in the defence against cold in the newborn harp seal.", "PMID": 953869}
{"id": "pubmed23n0032_764", "title": "Postjunctional supersensitivity of the rat vas deferens and gap junctions.", "content": "Tissues from the duodenum and vas deferens of Sprague-Dawley rats were examined of the rat vas deferens and gap junctions. Can. J. Physiol. Pharmacol. 54, 412-416. by electron microscopy after glutaraldehyde fixation and postosmication. Gap junctions (nexuses) were readily demonstrated in the duodenum in both control and reserpine treated animals (1.0 mg/kg per day for 7 days). Honever, gap junctions could not be demonstrated in vas deferens. It is concluded that the postjunctional supersensitivity and spontaneous activity induced by reserpine in vas deferens, does not result from the formation of gap junctions.", "contents": "Postjunctional supersensitivity of the rat vas deferens and gap junctions. Tissues from the duodenum and vas deferens of Sprague-Dawley rats were examined of the rat vas deferens and gap junctions. Can. J. Physiol. Pharmacol. 54, 412-416. by electron microscopy after glutaraldehyde fixation and postosmication. Gap junctions (nexuses) were readily demonstrated in the duodenum in both control and reserpine treated animals (1.0 mg/kg per day for 7 days). Honever, gap junctions could not be demonstrated in vas deferens. It is concluded that the postjunctional supersensitivity and spontaneous activity induced by reserpine in vas deferens, does not result from the formation of gap junctions.", "PMID": 953870}
{"id": "pubmed23n0032_765", "title": "Neurologic disorders responsive to folic acid therapy.", "content": "Six women aged 31 to 70 years had folate deficiency and neuropsychiatric disorders. The three with acquired folate deficiency were depressed and had permanent muscular and intellectual fatigue, mild symptoms of restless legs, depressed ankle jerks, diminution of vibration sensation in the legs, stocking-type hypoesthesia and long-lasting constipation; D-xylos absorption was abnormal. The bone marrow was megaloblastic in only one patient, and she and one other had atrophy of the jejunal mucosa. The third was a vegan. All three recovered after folic acid therapy. The other three were members of a family with the restless legs syndrome, fatigability and diffuse muscular pain. One also had subacute combined degeneration of the spinal cord and kidney disease but no megaloblastosis; she improved spectacularly after receiving large daily doses of folic acid. The other two also had minor neurologic signs, controlled with 5 to 10 mg of folic acid daily. Unrecognized and treatable folate deficiency (with low serum folic acid values but normal erythrocyte folate values) may be the basis of a well defined syndrome of neurologic, psychiatric and gastroenterologic disorders, and the restless legs syndrome may represent the main clinical expression of acquired and familial (or inborn) folate deficiency in adults.", "contents": "Neurologic disorders responsive to folic acid therapy. Six women aged 31 to 70 years had folate deficiency and neuropsychiatric disorders. The three with acquired folate deficiency were depressed and had permanent muscular and intellectual fatigue, mild symptoms of restless legs, depressed ankle jerks, diminution of vibration sensation in the legs, stocking-type hypoesthesia and long-lasting constipation; D-xylos absorption was abnormal. The bone marrow was megaloblastic in only one patient, and she and one other had atrophy of the jejunal mucosa. The third was a vegan. All three recovered after folic acid therapy. The other three were members of a family with the restless legs syndrome, fatigability and diffuse muscular pain. One also had subacute combined degeneration of the spinal cord and kidney disease but no megaloblastosis; she improved spectacularly after receiving large daily doses of folic acid. The other two also had minor neurologic signs, controlled with 5 to 10 mg of folic acid daily. Unrecognized and treatable folate deficiency (with low serum folic acid values but normal erythrocyte folate values) may be the basis of a well defined syndrome of neurologic, psychiatric and gastroenterologic disorders, and the restless legs syndrome may represent the main clinical expression of acquired and familial (or inborn) folate deficiency in adults.", "PMID": 953882}
{"id": "pubmed23n0032_766", "title": "Recurrent electromechanical dissociation due to severe underlying coronary heart disease.", "content": "Recurrent electromechanical dissociation, eventually fatal, presented as Stokes-Adams-like syncope in a patient with severe underlying coronary heart disease. Mechanical function was probably suppressed because of myocardial ischemia due to recent occlusion of the proximal right main coronary artery; the left main coronary artery had been occluded previously. Spasm of the proximal right coronary artery may have accounted for his recurrent attacks of pain and syncope. It is suggested that this clinical picture has a grave prognosis, demanding urgent therapeutic measures. While these measures are likely to be more hazardous than usual, the grave prognosis and potential for therapeutic success make them well worth the risk.", "contents": "Recurrent electromechanical dissociation due to severe underlying coronary heart disease. Recurrent electromechanical dissociation, eventually fatal, presented as Stokes-Adams-like syncope in a patient with severe underlying coronary heart disease. Mechanical function was probably suppressed because of myocardial ischemia due to recent occlusion of the proximal right main coronary artery; the left main coronary artery had been occluded previously. Spasm of the proximal right coronary artery may have accounted for his recurrent attacks of pain and syncope. It is suggested that this clinical picture has a grave prognosis, demanding urgent therapeutic measures. While these measures are likely to be more hazardous than usual, the grave prognosis and potential for therapeutic success make them well worth the risk.", "PMID": 953883}
{"id": "pubmed23n0032_767", "title": "Methylmalonic acidemia controlled with oral administration of vitamin B12.", "content": "A 3-month-old male infant had two episodes of fever, projectile vomiting, dehydration, generalized fine tremors and gross metabloic ketoacidosis. Methylmalonic acid was found in high concentration in both serum and urine, although the concentration of serum vitamin B12 was normal. A therapeutic trial of vitamin B12, administered parenterally, reduced greatly the methylmalonic aciduria. The patient has since been given vitamin B12 supplements continuously, initially 1 mg intramuscularly every other day, then 15 mg/d orally, and the protein in his diet was subsequently restricted. The most effected control of the methylmalonic aciduria was achieved with the combined regimen of oral vitamin therapy and dietary protein restriction. His physical and intellectual development have progressed normally and he has survived several acute respiratory tract infections without recurrence of metabolic acidosis.", "contents": "Methylmalonic acidemia controlled with oral administration of vitamin B12. A 3-month-old male infant had two episodes of fever, projectile vomiting, dehydration, generalized fine tremors and gross metabloic ketoacidosis. Methylmalonic acid was found in high concentration in both serum and urine, although the concentration of serum vitamin B12 was normal. A therapeutic trial of vitamin B12, administered parenterally, reduced greatly the methylmalonic aciduria. The patient has since been given vitamin B12 supplements continuously, initially 1 mg intramuscularly every other day, then 15 mg/d orally, and the protein in his diet was subsequently restricted. The most effected control of the methylmalonic aciduria was achieved with the combined regimen of oral vitamin therapy and dietary protein restriction. His physical and intellectual development have progressed normally and he has survived several acute respiratory tract infections without recurrence of metabolic acidosis.", "PMID": 953884}
{"id": "pubmed23n0032_768", "title": "Herpes simplex encephalitis: treatment with surgical decompression and cytosine arabinoside.", "content": "Herpes simplex encephalitis in a 21-year-old man presented as a flu-like illness, followed by inappropriate behaviour, drowsiness and focal neurologic signs. Investigations indicated a lesion in the right temporal lobe. The diagnosis was confirmed by isolation of the virus from a cerebral biopsy. Pronounced clinical improvement was noted when cytosine arabinoside therapy was started in the postoperative period. This report supports the observation by some authors that cytosine arabinoside may be of value in the treatment of herpes simplex encephalitis.", "contents": "Herpes simplex encephalitis: treatment with surgical decompression and cytosine arabinoside. Herpes simplex encephalitis in a 21-year-old man presented as a flu-like illness, followed by inappropriate behaviour, drowsiness and focal neurologic signs. Investigations indicated a lesion in the right temporal lobe. The diagnosis was confirmed by isolation of the virus from a cerebral biopsy. Pronounced clinical improvement was noted when cytosine arabinoside therapy was started in the postoperative period. This report supports the observation by some authors that cytosine arabinoside may be of value in the treatment of herpes simplex encephalitis.", "PMID": 953885}
{"id": "pubmed23n0032_769", "title": "Effect of physical training on hemodynamic performance following myocardial infarction: a controlled study.", "content": "The effect of physical training on hemodynamic performance was evaluated in a group of patients who had had a myocardial infarction and a group of healthy, age-matched controls. Before training, the patients' mean ventilatory equivalent was significantly less than that of the controls at the lowest workload (300 kpm/min), the mean stroke volume was significantly increased at the highest workload then achieved (600 kpm/min), and the mean arteriovenous oxygen content difference was significantly smaller at the highest workload. The patients had a relative bradycardia before training and there was no significant reduction in mean resting or submaximal heart rate after training. Their mean oxygen uptake was significantly reduced at the lowest exercise workload after training and this response was significantly different from that of the controls after 8 weeks of training. Mean cardiac output during exercise was significantly reduced in the patients after training, but only at the 600-kpm/min workload, the response being blunted at 900 kpm/min; mean stroke volume was also significantly reduced at this workload after training; both these responses were significantly different from those of the controls Mean arteriovenous oxygen content difference at 6oo kpm/min was significantly increased in the patients after training, though the response was not significantly different from that of the controls. Mean ventilatory equivalent was also significantly increased in patients after training, becoming similar to that of the controls.", "contents": "Effect of physical training on hemodynamic performance following myocardial infarction: a controlled study. The effect of physical training on hemodynamic performance was evaluated in a group of patients who had had a myocardial infarction and a group of healthy, age-matched controls. Before training, the patients' mean ventilatory equivalent was significantly less than that of the controls at the lowest workload (300 kpm/min), the mean stroke volume was significantly increased at the highest workload then achieved (600 kpm/min), and the mean arteriovenous oxygen content difference was significantly smaller at the highest workload. The patients had a relative bradycardia before training and there was no significant reduction in mean resting or submaximal heart rate after training. Their mean oxygen uptake was significantly reduced at the lowest exercise workload after training and this response was significantly different from that of the controls after 8 weeks of training. Mean cardiac output during exercise was significantly reduced in the patients after training, but only at the 600-kpm/min workload, the response being blunted at 900 kpm/min; mean stroke volume was also significantly reduced at this workload after training; both these responses were significantly different from those of the controls Mean arteriovenous oxygen content difference at 6oo kpm/min was significantly increased in the patients after training, though the response was not significantly different from that of the controls. Mean ventilatory equivalent was also significantly increased in patients after training, becoming similar to that of the controls.", "PMID": 953886}
{"id": "pubmed23n0032_770", "title": "Psychiatric illness in the medical profession: incidence in relation to sex and field of practice.", "content": "The overall incidence of psychiatric illness among the physicians of British Columbia during 1970-74 was 1.27% per year. The overall suicide rate was more than 36.5/100 000. Incidence was not dependent on sex or age. The two specialties with the highest incidence--ophthalmology and psychiatry--had previously been demonstrated to have significantly high rates of suicide. The highest incidence was among psychiatric residents; in other resident groups collectively the incidence was at the expected rate. Greater severity of illness and poorer prognosis was found in family physicians compared with specialists, although the incidence was the same in the two groups.", "contents": "Psychiatric illness in the medical profession: incidence in relation to sex and field of practice. The overall incidence of psychiatric illness among the physicians of British Columbia during 1970-74 was 1.27% per year. The overall suicide rate was more than 36.5/100 000. Incidence was not dependent on sex or age. The two specialties with the highest incidence--ophthalmology and psychiatry--had previously been demonstrated to have significantly high rates of suicide. The highest incidence was among psychiatric residents; in other resident groups collectively the incidence was at the expected rate. Greater severity of illness and poorer prognosis was found in family physicians compared with specialists, although the incidence was the same in the two groups.", "PMID": 953898}
{"id": "pubmed23n0032_771", "title": "Sex therapy: considerations in the selection of patients.", "content": "Sex therapy has proven helpful for many patients with sexual dysfunctions. In judging the appropriateness of this new treatment procedure the clinician should determine the following: that a sexual dysfunction exists, that a stable couple relationship exists, that there are no major marital, psychiatric or physical problems that are etiologically important or that would interfere with treatment, and that both partners are willing to engage in a treatment program.", "contents": "Sex therapy: considerations in the selection of patients. Sex therapy has proven helpful for many patients with sexual dysfunctions. In judging the appropriateness of this new treatment procedure the clinician should determine the following: that a sexual dysfunction exists, that a stable couple relationship exists, that there are no major marital, psychiatric or physical problems that are etiologically important or that would interfere with treatment, and that both partners are willing to engage in a treatment program.", "PMID": 953899}
{"id": "pubmed23n0032_772", "title": "Hospitalization for mental illness: evaluation of admission trends from 1941 to 1971.", "content": "A study of the diagnostic composition of the inpatient population of Ontario and Canadian psychiatric facilities has shown an important change in hospital-treated illness over the period 1941-71. Patients with nonpsychotic disorders accounted for 54% of all admissions to Ontario public mental hospitals in 1971, compared with only 8% in 1941. The trend was similar for both first admissions and proportion of readmissions, and was similar for psychiatric units of general hospitals. In contrast, the overall rate of first admission for psychotic disorders to inpatient facilities remained remarkably constant over time, as did the proportion of readmissions among all admissions. The findings dispel the notion that the increasing proportion of readmissions is due largely to a rapid turnover of former long-stay psychotic patients (the \"revolving-door phenomenon\"). The findings could not be attributed to a changing prevalence of types of psychiatric illness, increased availability of psychiatric inpatient facilities or comprehensive medical insurance.", "contents": "Hospitalization for mental illness: evaluation of admission trends from 1941 to 1971. A study of the diagnostic composition of the inpatient population of Ontario and Canadian psychiatric facilities has shown an important change in hospital-treated illness over the period 1941-71. Patients with nonpsychotic disorders accounted for 54% of all admissions to Ontario public mental hospitals in 1971, compared with only 8% in 1941. The trend was similar for both first admissions and proportion of readmissions, and was similar for psychiatric units of general hospitals. In contrast, the overall rate of first admission for psychotic disorders to inpatient facilities remained remarkably constant over time, as did the proportion of readmissions among all admissions. The findings dispel the notion that the increasing proportion of readmissions is due largely to a rapid turnover of former long-stay psychotic patients (the \"revolving-door phenomenon\"). The findings could not be attributed to a changing prevalence of types of psychiatric illness, increased availability of psychiatric inpatient facilities or comprehensive medical insurance.", "PMID": 953900}
{"id": "pubmed23n0032_773", "title": "Endobronchial lipoma: two cases and review of the literature.", "content": "Endobronchial lipoma is a benign tumour of the large bronchi occurring in middle-aged men. To the 38 successfully treated cases in the English literature a further 2 are added. The symptoms are those of obstructive pneumonitis mimicking bronchogenic carcinoma, and the result of delayed therapy may be bronchiectasis. Treatment includes local resection through a bronchoscope or a bronchotomy incision, or removal, if necessary, of the obstructed lobe or lung at thoracotomy. Smoking may be important in the pathogenesis of this tumour.", "contents": "Endobronchial lipoma: two cases and review of the literature. Endobronchial lipoma is a benign tumour of the large bronchi occurring in middle-aged men. To the 38 successfully treated cases in the English literature a further 2 are added. The symptoms are those of obstructive pneumonitis mimicking bronchogenic carcinoma, and the result of delayed therapy may be bronchiectasis. Treatment includes local resection through a bronchoscope or a bronchotomy incision, or removal, if necessary, of the obstructed lobe or lung at thoracotomy. Smoking may be important in the pathogenesis of this tumour.", "PMID": 953901}
{"id": "pubmed23n0032_774", "title": "Airway obstruction following smoke inhalation.", "content": "Respiratory distress with episodes of cyanosis, intercostal retraction and sibilant rhonchi occurred in a 2-year-old boy over a 48-hour period following serious smoke inhalation. Worsening of the child's condition accompanied these findings, culminating in sudden loss of air entry, severe respiratory distress and loss of consciousness, which necessitated endotracheal intubation for resuscitation. Pronounced improvement followed removal of two pseudomembranous bronchial casts from the airway by suctioning, and thereafter recovery was uneventful.", "contents": "Airway obstruction following smoke inhalation. Respiratory distress with episodes of cyanosis, intercostal retraction and sibilant rhonchi occurred in a 2-year-old boy over a 48-hour period following serious smoke inhalation. Worsening of the child's condition accompanied these findings, culminating in sudden loss of air entry, severe respiratory distress and loss of consciousness, which necessitated endotracheal intubation for resuscitation. Pronounced improvement followed removal of two pseudomembranous bronchial casts from the airway by suctioning, and thereafter recovery was uneventful.", "PMID": 953902}
{"id": "pubmed23n0032_775", "title": "Tartrazine-containing drugs.", "content": "Pharmaceutical manufacturers producing or distributing drugs in Canada were surveyed between December 1974 and March 1975 to determine which of their products contained tartrazine, a pyrazole aniline dye. A list of some 580 drug products of the 156 manufacturers who responded is presented for aid in managing the tartrazine-sensitive patients.", "contents": "Tartrazine-containing drugs. Pharmaceutical manufacturers producing or distributing drugs in Canada were surveyed between December 1974 and March 1975 to determine which of their products contained tartrazine, a pyrazole aniline dye. A list of some 580 drug products of the 156 manufacturers who responded is presented for aid in managing the tartrazine-sensitive patients.", "PMID": 953903}
{"id": "pubmed23n0032_776", "title": "Triiodothyronine and thyroxine interrelationships in health and disease.", "content": "With the recent development of radioimmunoassay techniques for the measurement of serum triiodothyronine (T3) concentration, new concepts have arisen regarding the biologic role of T3 in health and disease and its interrelationships with thyroxine (T4). An awareness of the influence of clinical conditions that affect binding of thyroid hormone to plasma proteins is required in the interpretation of moderately increased or decreased serum T3 values. Hormone preparations containing T3 may produce transient increases in T3 concentration into the hyperthyroid range. Measurements of serum T3 concentration appear to be particularly indicated in clinical situations in which hyperthyroidism is suspected but serum T3 resin uptake and serum T4 values are normal, to exclude the T3-toxicosis syndrome. Also, when serum T4 values are in the hypothyroid range, measurement of serum T3 as well as serum thyrotropin (TSH) concentrations can lead to recognition of abnormalities in thyroid gland biosynthesis. Before a diagnosis of hypothyroidism is made on the basis of a low serum T3 value, one must exclude a variety of clinical nonthyroidal conditions that can result in changes in plasma T3 protein binding or impaired peripheral conversion of T4 to metabolically active T3 without producing a hypometabolic state.", "contents": "Triiodothyronine and thyroxine interrelationships in health and disease. With the recent development of radioimmunoassay techniques for the measurement of serum triiodothyronine (T3) concentration, new concepts have arisen regarding the biologic role of T3 in health and disease and its interrelationships with thyroxine (T4). An awareness of the influence of clinical conditions that affect binding of thyroid hormone to plasma proteins is required in the interpretation of moderately increased or decreased serum T3 values. Hormone preparations containing T3 may produce transient increases in T3 concentration into the hyperthyroid range. Measurements of serum T3 concentration appear to be particularly indicated in clinical situations in which hyperthyroidism is suspected but serum T3 resin uptake and serum T4 values are normal, to exclude the T3-toxicosis syndrome. Also, when serum T4 values are in the hypothyroid range, measurement of serum T3 as well as serum thyrotropin (TSH) concentrations can lead to recognition of abnormalities in thyroid gland biosynthesis. Before a diagnosis of hypothyroidism is made on the basis of a low serum T3 value, one must exclude a variety of clinical nonthyroidal conditions that can result in changes in plasma T3 protein binding or impaired peripheral conversion of T4 to metabolically active T3 without producing a hypometabolic state.", "PMID": 953904}
{"id": "pubmed23n0032_777", "title": "A case of cholera in Kingston, Ont.", "content": "A case of cholera occurred in Kingston, Ont. in 1974 in a traveller from South Africa. Treatment, based on an understanding of the pathophysiology of cholera diarrhea and the mechanism of action of the Vibrio cholerae enterotoxin on gastrointestinal fluid loss, consisted of correcting the severe loss of fluid and electrolytes and the metabolic acidosis, as soon as the patient could tolerate taking fluids orally, further fluid replacement consisted increasingly of oral administration of glucose and saline. Tetracycline therapy was given only to shorten the duration of the acute illness.", "contents": "A case of cholera in Kingston, Ont. A case of cholera occurred in Kingston, Ont. in 1974 in a traveller from South Africa. Treatment, based on an understanding of the pathophysiology of cholera diarrhea and the mechanism of action of the Vibrio cholerae enterotoxin on gastrointestinal fluid loss, consisted of correcting the severe loss of fluid and electrolytes and the metabolic acidosis, as soon as the patient could tolerate taking fluids orally, further fluid replacement consisted increasingly of oral administration of glucose and saline. Tetracycline therapy was given only to shorten the duration of the acute illness.", "PMID": 953912}
{"id": "pubmed23n0032_778", "title": "Laboratory investigation and infection control of cholera in Kingston, Ont.", "content": "Vibrio cholerae, biotype El Tor, was isolated in a hospital laboratory in Kingston, Ont. in 1974. Confirmation and complete identification by the Ontario regional and provincial public health laboratories was obtained within 3 days. Institution of well established infection-control and public health measures prevented spread of the infection within the hospital and the community.", "contents": "Laboratory investigation and infection control of cholera in Kingston, Ont. Vibrio cholerae, biotype El Tor, was isolated in a hospital laboratory in Kingston, Ont. in 1974. Confirmation and complete identification by the Ontario regional and provincial public health laboratories was obtained within 3 days. Institution of well established infection-control and public health measures prevented spread of the infection within the hospital and the community.", "PMID": 953913}
{"id": "pubmed23n0032_779", "title": "Cholera: synopsis of clinical aspects and principles of treatment.", "content": "Cholera varies greatly in clinical severity; the mortality of untreated severe cholera may be as high as 60% The main clinical feature is dehydration; fluid lost in the stools may amount to 60/. Rehydration is the cornerstone of treatment. The amount of fluid required is approximately 10% of body weight in severe dehydration and 5 to 8% in moderate dehydration. Fluid therapy, which must be individualized, may be successful on its own, but chemo-therapy shortens the duration of illness. Tetracycline (in adults, 40 mg/kg for 2 days; in children, 50 mg/kg for 2 days) reduces the fluid loss and eliminates the causative organisms. Vaccination is of limited value.", "contents": "Cholera: synopsis of clinical aspects and principles of treatment. Cholera varies greatly in clinical severity; the mortality of untreated severe cholera may be as high as 60% The main clinical feature is dehydration; fluid lost in the stools may amount to 60/. Rehydration is the cornerstone of treatment. The amount of fluid required is approximately 10% of body weight in severe dehydration and 5 to 8% in moderate dehydration. Fluid therapy, which must be individualized, may be successful on its own, but chemo-therapy shortens the duration of illness. Tetracycline (in adults, 40 mg/kg for 2 days; in children, 50 mg/kg for 2 days) reduces the fluid loss and eliminates the causative organisms. Vaccination is of limited value.", "PMID": 953914}
{"id": "pubmed23n0032_780", "title": "Cardiac hemolytic anemia resolving after second mitral annuloplasty.", "content": "Following an episode of rheumatic carditis, severe mitral incompetence developed in a 9-year-old girl. A mitral annuloplasty succeeded for a short time in ameliorating her symptoms of cardiac failure. However, mitral incompetence recurred and was accompanied by severe anemia and hemosiderinuria. Distortion of erythrocytes was evident on a peripheral blood smear. A second mitral annuloplasty resulted in resolution of the hemolytic anemia.", "contents": "Cardiac hemolytic anemia resolving after second mitral annuloplasty. Following an episode of rheumatic carditis, severe mitral incompetence developed in a 9-year-old girl. A mitral annuloplasty succeeded for a short time in ameliorating her symptoms of cardiac failure. However, mitral incompetence recurred and was accompanied by severe anemia and hemosiderinuria. Distortion of erythrocytes was evident on a peripheral blood smear. A second mitral annuloplasty resulted in resolution of the hemolytic anemia.", "PMID": 953916}
{"id": "pubmed23n0032_781", "title": "Coarctation of the aorta in adults.", "content": "Thirty-six patients, 19 men and 17 women, presented at age 18 or older between 1952 and 1974 with coarctation of the aorta. Of the 14 (39%) who had associated cardiovascular disease, 12 had aortic stenosis or insufficiency or both. Three patients had infections-two, endocarditis (aortic valve) and one, endarteritis. Three of the seven patients who did not undergo an operation are alive, two at more than 50 years of age. Five patients had myocardial infarctions, two at 35 years of age. Twenty-nine (80%) had operations; in eight instances the patient was over age 40. All 18 patients undergoing repair of isolated coarctation survived, while only 7 of the 11 patients with associated cardiovascular lesions who underwent repair recovered. Aortic valvular disease and myocardial infarction are serious complicating factors in coarctation of the aorta.", "contents": "Coarctation of the aorta in adults. Thirty-six patients, 19 men and 17 women, presented at age 18 or older between 1952 and 1974 with coarctation of the aorta. Of the 14 (39%) who had associated cardiovascular disease, 12 had aortic stenosis or insufficiency or both. Three patients had infections-two, endocarditis (aortic valve) and one, endarteritis. Three of the seven patients who did not undergo an operation are alive, two at more than 50 years of age. Five patients had myocardial infarctions, two at 35 years of age. Twenty-nine (80%) had operations; in eight instances the patient was over age 40. All 18 patients undergoing repair of isolated coarctation survived, while only 7 of the 11 patients with associated cardiovascular lesions who underwent repair recovered. Aortic valvular disease and myocardial infarction are serious complicating factors in coarctation of the aorta.", "PMID": 953915}
{"id": "pubmed23n0032_782", "title": "Design and evaluation of head unit for wheelchair control by quadriplegic patients.", "content": "A control unit was designed to allow persons who have lost hand and arm function to control the speed, steering, reversal and on-off switching of an electric wheelchair by means of backward movement and rotation of the head. When possible, shoulder movement was used to control both reversal and on-off switching. Clinical evaluation in 10 patients with quadriplegia and 2 with severe neuromuscular disease showed that the unit neither interfered with nor restrained the patients' residual body movements, permitted use of natural head movements for smooth and fast control of the wheelchair, and was well accepted by and integrated into the life of the patients.", "contents": "Design and evaluation of head unit for wheelchair control by quadriplegic patients. A control unit was designed to allow persons who have lost hand and arm function to control the speed, steering, reversal and on-off switching of an electric wheelchair by means of backward movement and rotation of the head. When possible, shoulder movement was used to control both reversal and on-off switching. Clinical evaluation in 10 patients with quadriplegia and 2 with severe neuromuscular disease showed that the unit neither interfered with nor restrained the patients' residual body movements, permitted use of natural head movements for smooth and fast control of the wheelchair, and was well accepted by and integrated into the life of the patients.", "PMID": 953917}
{"id": "pubmed23n0032_783", "title": "Hodgkin's disease following thorium dioxide angiography.", "content": "Hodgkin's disease occurred in a 53-year-old man who, 25 years previously, had undergone cerebral angiography, for which thorium dioxide suspension (Thorotrast) was used. Deposits of throium dioxide were noted in reticuloendothelial cells in various locations. An association between thorium dioxide administration and the subsequent development of malignant tumours and neoplastic hematologic disorders has previously been reported.", "contents": "Hodgkin's disease following thorium dioxide angiography. Hodgkin's disease occurred in a 53-year-old man who, 25 years previously, had undergone cerebral angiography, for which thorium dioxide suspension (Thorotrast) was used. Deposits of throium dioxide were noted in reticuloendothelial cells in various locations. An association between thorium dioxide administration and the subsequent development of malignant tumours and neoplastic hematologic disorders has previously been reported.", "PMID": 953918}
{"id": "pubmed23n0032_784", "title": "Transmission of hepatitis B by a human bite: an occupational hazard.", "content": "Hepatitis B developed in a policeman 15 weeks after he was bitten on the hand. A few days after the bite hepatitis B developed in the assailant. The bite had drawn blood and this method of inoculation was presumed to be the route of transmission of the virus. Compensation was awarded on the grounds that this was an occupationally acquired disease.", "contents": "Transmission of hepatitis B by a human bite: an occupational hazard. Hepatitis B developed in a policeman 15 weeks after he was bitten on the hand. A few days after the bite hepatitis B developed in the assailant. The bite had drawn blood and this method of inoculation was presumed to be the route of transmission of the virus. Compensation was awarded on the grounds that this was an occupationally acquired disease.", "PMID": 953919}
{"id": "pubmed23n0032_785", "title": "Scabies masquerading as Letterer-Siwe's disease.", "content": "Letterer-Siwe's disease was diagnosed from clinical appearance and initial assessment of a skin biopsy in a child with a 2-month history of skin rash. Fine erythematous papules were scattered on the trunk. The biopsy showed epidermal thickening and an inflammatory infiltrate chiefly in the upper layers of the dermis; deeper in the dermis the infiltrate was perivascular and periappendicular, histiocytes predominating in some areas and lymphocytes in others. A diagnosis of scabies was made after burrows were demonstrated on palms and soles and the mite of scabies was isolated from them.", "contents": "Scabies masquerading as Letterer-Siwe's disease. Letterer-Siwe's disease was diagnosed from clinical appearance and initial assessment of a skin biopsy in a child with a 2-month history of skin rash. Fine erythematous papules were scattered on the trunk. The biopsy showed epidermal thickening and an inflammatory infiltrate chiefly in the upper layers of the dermis; deeper in the dermis the infiltrate was perivascular and periappendicular, histiocytes predominating in some areas and lymphocytes in others. A diagnosis of scabies was made after burrows were demonstrated on palms and soles and the mite of scabies was isolated from them.", "PMID": 953920}
{"id": "pubmed23n0032_786", "title": "Phenylketonuria variants in Ontario.", "content": "Since mass screening of the newborn population for phenylketonuria (PKU) by the Guthrie test was begun in Ontario in July 1965 many variants of PKU have been recognized in the 96 to 97% screened. Seventy-one cases of classic PKU were detected (four were missed). Of 48 cases of persistent hyperphenylalaninemia discovered, 18 were classified as atypical PKU and 30 as persistent benign hyperphenylalaninemia. Numerous infants with transient hyperphenylalaninemia (initial values over 10 mg/dl in 12), in many instances the result of transient neonatal tyrosinemia, were discovered. There was a slight predominance of males. Serum phenylalanine values of up to 15 mg/dl seemed to be harmless to the developing brain. A survey of 67 247 adults in the general population revealed 1 person with PKU and 1 with persistent benign hyperphenylalaninemia; both had normal intelligence quotients. Of 1548 mothers of retarded children tested, none had hyperphenylalaninemia.", "contents": "Phenylketonuria variants in Ontario. Since mass screening of the newborn population for phenylketonuria (PKU) by the Guthrie test was begun in Ontario in July 1965 many variants of PKU have been recognized in the 96 to 97% screened. Seventy-one cases of classic PKU were detected (four were missed). Of 48 cases of persistent hyperphenylalaninemia discovered, 18 were classified as atypical PKU and 30 as persistent benign hyperphenylalaninemia. Numerous infants with transient hyperphenylalaninemia (initial values over 10 mg/dl in 12), in many instances the result of transient neonatal tyrosinemia, were discovered. There was a slight predominance of males. Serum phenylalanine values of up to 15 mg/dl seemed to be harmless to the developing brain. A survey of 67 247 adults in the general population revealed 1 person with PKU and 1 with persistent benign hyperphenylalaninemia; both had normal intelligence quotients. Of 1548 mothers of retarded children tested, none had hyperphenylalaninemia.", "PMID": 953933}
{"id": "pubmed23n0032_787", "title": "Removal of amantadine hydrochloride by dialysis in patients with renal insufficiency.", "content": "Two patients with Parkinson's disease and renal insufficiency had excessively high concentrations of amantadine hydrochloride in the blood. The amounts of the drug removed by hemodialysis and peritoneal dialysis were small. However, since extrarenal elimination is negligible in such patients, frequently repeated dialysis may be required to remove the drug.", "contents": "Removal of amantadine hydrochloride by dialysis in patients with renal insufficiency. Two patients with Parkinson's disease and renal insufficiency had excessively high concentrations of amantadine hydrochloride in the blood. The amounts of the drug removed by hemodialysis and peritoneal dialysis were small. However, since extrarenal elimination is negligible in such patients, frequently repeated dialysis may be required to remove the drug.", "PMID": 953934}
{"id": "pubmed23n0032_788", "title": "Restaging of breast cancer in a northern Alberta registry.", "content": "All breast cancer patients presenting preoperatively at the Dr. W.W. Cross Cancer Institute, Edmonton, since 1971 are included in a registry that provides detailed clinical and epidemiologic information on the nature of this disease in northern Alberta. For the 141 female patients who were seen preoperatively in 1971 and thereafter followed regularly, disease was restaged according to the 1973 revision of the tumour-nodes-metastases (TNM) formula of Union internationale contre le cancer (UICC) to permit comparison with staging according to the 1968 formula. The staging distribution was altered greatly because of major changes in the staging system. With the 1973 revision stage II includes disease without palpable lymph nodes and greater emphasis is placed on tumour size. Accurate preoperative staging and registration are essential for meaningful statistics and as an aid in patient management.", "contents": "Restaging of breast cancer in a northern Alberta registry. All breast cancer patients presenting preoperatively at the Dr. W.W. Cross Cancer Institute, Edmonton, since 1971 are included in a registry that provides detailed clinical and epidemiologic information on the nature of this disease in northern Alberta. For the 141 female patients who were seen preoperatively in 1971 and thereafter followed regularly, disease was restaged according to the 1973 revision of the tumour-nodes-metastases (TNM) formula of Union internationale contre le cancer (UICC) to permit comparison with staging according to the 1968 formula. The staging distribution was altered greatly because of major changes in the staging system. With the 1973 revision stage II includes disease without palpable lymph nodes and greater emphasis is placed on tumour size. Accurate preoperative staging and registration are essential for meaningful statistics and as an aid in patient management.", "PMID": 953935}
{"id": "pubmed23n0032_789", "title": "Management of the rape victim.", "content": "A woman's response to rape can be divided into three phases: an acute reaction, an intermediate stage and a period of resolution. Proper management of the physical and emotional problems of each phase, ideally by the woman's family doctor or gynecologist, may prevent future problems. Treatment during the first phase includes responding to the emotional needs of the patient as well as doing a pelvic and general physical examination to detect any injuries; information for possible legal procedures may be obtained quickly and efficiently. Follow-up particularly psychological, is important in the second and third phases.", "contents": "Management of the rape victim. A woman's response to rape can be divided into three phases: an acute reaction, an intermediate stage and a period of resolution. Proper management of the physical and emotional problems of each phase, ideally by the woman's family doctor or gynecologist, may prevent future problems. Treatment during the first phase includes responding to the emotional needs of the patient as well as doing a pelvic and general physical examination to detect any injuries; information for possible legal procedures may be obtained quickly and efficiently. Follow-up particularly psychological, is important in the second and third phases.", "PMID": 953936}
{"id": "pubmed23n0032_790", "title": "Don't forget the children.", "content": "Serious illness or hospitalization of a parent is potentially destructive to the mental health of the child. Stress is caused by both the separation and the illness itself. If the physician does not ensure that the situation is explained to the child, it is likely the child will be confused and frightened and any attempts at clarification will be blocked in the attendant family dysfunction. The general principles of prevention are to share the facts of the situation with the child and to allow him his natural and varied reactions. The family interview is useful in clearing up misunderstandings, sharing feelings and working out family roles.", "contents": "Don't forget the children. Serious illness or hospitalization of a parent is potentially destructive to the mental health of the child. Stress is caused by both the separation and the illness itself. If the physician does not ensure that the situation is explained to the child, it is likely the child will be confused and frightened and any attempts at clarification will be blocked in the attendant family dysfunction. The general principles of prevention are to share the facts of the situation with the child and to allow him his natural and varied reactions. The family interview is useful in clearing up misunderstandings, sharing feelings and working out family roles.", "PMID": 953937}
{"id": "pubmed23n0032_791", "title": "Occurrence of two cases of cryptococcosis within 3 months on the same general medical ward.", "content": "Two cases of systemic cryptococcal infection occurred on the same general medical ward within 3 months. The patients had been on the same ward for 5 days. Both had other diseases associated with depression of cellular immune responses had received immunosuppressive drugs.", "contents": "Occurrence of two cases of cryptococcosis within 3 months on the same general medical ward. Two cases of systemic cryptococcal infection occurred on the same general medical ward within 3 months. The patients had been on the same ward for 5 days. Both had other diseases associated with depression of cellular immune responses had received immunosuppressive drugs.", "PMID": 953938}
{"id": "pubmed23n0032_792", "title": "Which neuroses need specialist care?", "content": "In order to differentiate the neurotic patient who both needs and responds to psychiatric care from the majority of neurotic patients, who do not need this, carefully matched pairs of neurotic patients being treated at psychiatric and nonpsychiatric clinics in Montreal were followed up for 1 year. Improvement was substantial regardless of treatment, and the psychiatrically treated, on the whole, improved only slightly more than the others. However, one type of patient improved greatly under psychiatric care while improving almost not at all without it--introverts who considered themselves unhealthy but found life manageable, had avoided taking time off work or using anxiolytic drugs and appeared to handle their frustrations without repressing their irritation or losing self-control. It is suggested that it is mainly this type of neurotic that should receive specialist referral.", "contents": "Which neuroses need specialist care? In order to differentiate the neurotic patient who both needs and responds to psychiatric care from the majority of neurotic patients, who do not need this, carefully matched pairs of neurotic patients being treated at psychiatric and nonpsychiatric clinics in Montreal were followed up for 1 year. Improvement was substantial regardless of treatment, and the psychiatrically treated, on the whole, improved only slightly more than the others. However, one type of patient improved greatly under psychiatric care while improving almost not at all without it--introverts who considered themselves unhealthy but found life manageable, had avoided taking time off work or using anxiolytic drugs and appeared to handle their frustrations without repressing their irritation or losing self-control. It is suggested that it is mainly this type of neurotic that should receive specialist referral.", "PMID": 953939}
{"id": "pubmed23n0032_793", "title": "Masculinizing granulosa cell tumour.", "content": "A 23-year-old woman had oligomenorrhea, underdevelopment of the breasts, moderate hirsutism and increased serum testosterone values associated with a benign noncystic granulosa cell tumour of the left ovary. She was frail, irritable and apathetic. Since the age of 7 she had had periodic abdominal pain with nausea, vomiting and dizziness; irritability and occipital headache appeared when she was older. Her symptoms resolved and the masculinization did not progress after the tumour was removed. Only six similar well documented cases have been reported.", "contents": "Masculinizing granulosa cell tumour. A 23-year-old woman had oligomenorrhea, underdevelopment of the breasts, moderate hirsutism and increased serum testosterone values associated with a benign noncystic granulosa cell tumour of the left ovary. She was frail, irritable and apathetic. Since the age of 7 she had had periodic abdominal pain with nausea, vomiting and dizziness; irritability and occipital headache appeared when she was older. Her symptoms resolved and the masculinization did not progress after the tumour was removed. Only six similar well documented cases have been reported.", "PMID": 953940}
{"id": "pubmed23n0032_794", "title": "Symposium--psychiatric education.", "content": "The process of learning in groups, as exemplified by the author's experience in a multidiscipline conference on a surgical ward of a general hospital has been examined. A sequence of six developmental states has been identified: initial ambivalence; passive receptivity; resistance; task orientation; the work group and consolidation. These findings have been compared with those of other studies dealing with sequential stages in group development. The psychiatric consultant's role in expediting these phases and some of the difficulties encountered have been mentioned.", "contents": "Symposium--psychiatric education. The process of learning in groups, as exemplified by the author's experience in a multidiscipline conference on a surgical ward of a general hospital has been examined. A sequence of six developmental states has been identified: initial ambivalence; passive receptivity; resistance; task orientation; the work group and consolidation. These findings have been compared with those of other studies dealing with sequential stages in group development. The psychiatric consultant's role in expediting these phases and some of the difficulties encountered have been mentioned.", "PMID": 953947}
{"id": "pubmed23n0032_795", "title": "Supervision of psychotherapy with psychiatric residents. 1 model.", "content": "Training of psychiatric residents should include supervised experience in psychotherapy, whatever the mode of practice the psychiatrist-to-be will later choose. Individual supervision of psychotherapy on patients seen at least once a week for a year offers the best opportunity for learning in this area. The supervisor has a teaching role which includes showing the resident how to look more objectively and insightfully at the material, serving as a partial model of a professional identity, and giving direct didactic help regarding the subject of psychotherapy. Such an experience leads togrowth and development of the resident in his professional role as psychotherapist.", "contents": "Supervision of psychotherapy with psychiatric residents. 1 model. Training of psychiatric residents should include supervised experience in psychotherapy, whatever the mode of practice the psychiatrist-to-be will later choose. Individual supervision of psychotherapy on patients seen at least once a week for a year offers the best opportunity for learning in this area. The supervisor has a teaching role which includes showing the resident how to look more objectively and insightfully at the material, serving as a partial model of a professional identity, and giving direct didactic help regarding the subject of psychotherapy. Such an experience leads togrowth and development of the resident in his professional role as psychotherapist.", "PMID": 953948}
{"id": "pubmed23n0032_796", "title": "Utilizing reflected countertransference Applying the reflection process as a teaching tool in supervision.", "content": "There are times when teaching cannot proceed through the abstract presentation of content and must progress by the demonstration of what is to be taught. This is true whenever a student is unable, for whatever reason, to appropriately apply such abstract material to the concrete task before him. This may be due to a number of factors, one of the most common of which is the lack of qualitatively similar experiences to which to refer. The teaching of complicated processes such as psychotherapy is an example, especially when the trainee is relatively unsophisticated and/or seems unable to abstract from discussion of blocking phenomena, where it may become necessary to \"act in\" the feeling tones which are to be the cues for the behaviour to be learned, instead of persisting with abstract interpretations. When, in supervision, a supervisee recreates the conditions existing in the therapeutic situation, this procedural behaviour is called the \"Reflection Process\". This process often appears to be unconsciously motivated by the need of the supervisee to solicit from the supervisor a practical demonstration which he does not know how to solicit otherwise. Even if the Reflection Process is due mainly to unresolved, unconscious conflicts of the supervisee, it can be a useful clue as to what transference-countertransference problems he is having difficulties with. The fact that such behaviour on the part of the supervisee may arise out of unresolved unconscious conflictual material should not become a reason for dismissing it. Instead, supervisors should become proficient at recognizing the countertransferential feelings in themselves, which can be an accurate reflection of the feelings troubling the supervisee in his dealings with this patient; and they should recognize the distress signal on the part of the supervisee, which can often be responded to constructively. In the supervision, the supervisor at times can, almost unwittingly, come to play the role which the supervised therapists plays in the supervised therapy and, if not aware of the Reflector Process, he may not realize that the countertransferential feelings which underly his assuming that role may not be primarily a response to the character of the therapist he is supervising but rather to that of the patient. They may also be a reflection of the process problem in the supervised therapy. It is not essential that this process be labeled in the supervision; what is essential is that its ramifications be recognized by the supervisor.", "contents": "Utilizing reflected countertransference Applying the reflection process as a teaching tool in supervision. There are times when teaching cannot proceed through the abstract presentation of content and must progress by the demonstration of what is to be taught. This is true whenever a student is unable, for whatever reason, to appropriately apply such abstract material to the concrete task before him. This may be due to a number of factors, one of the most common of which is the lack of qualitatively similar experiences to which to refer. The teaching of complicated processes such as psychotherapy is an example, especially when the trainee is relatively unsophisticated and/or seems unable to abstract from discussion of blocking phenomena, where it may become necessary to \"act in\" the feeling tones which are to be the cues for the behaviour to be learned, instead of persisting with abstract interpretations. When, in supervision, a supervisee recreates the conditions existing in the therapeutic situation, this procedural behaviour is called the \"Reflection Process\". This process often appears to be unconsciously motivated by the need of the supervisee to solicit from the supervisor a practical demonstration which he does not know how to solicit otherwise. Even if the Reflection Process is due mainly to unresolved, unconscious conflicts of the supervisee, it can be a useful clue as to what transference-countertransference problems he is having difficulties with. The fact that such behaviour on the part of the supervisee may arise out of unresolved unconscious conflictual material should not become a reason for dismissing it. Instead, supervisors should become proficient at recognizing the countertransferential feelings in themselves, which can be an accurate reflection of the feelings troubling the supervisee in his dealings with this patient; and they should recognize the distress signal on the part of the supervisee, which can often be responded to constructively. In the supervision, the supervisor at times can, almost unwittingly, come to play the role which the supervised therapists plays in the supervised therapy and, if not aware of the Reflector Process, he may not realize that the countertransferential feelings which underly his assuming that role may not be primarily a response to the character of the therapist he is supervising but rather to that of the patient. They may also be a reflection of the process problem in the supervised therapy. It is not essential that this process be labeled in the supervision; what is essential is that its ramifications be recognized by the supervisor.", "PMID": 953949}
{"id": "pubmed23n0032_797", "title": "SELF-INDUCED TELEVISION EPILEPSY. A study of 2 patients.", "content": "A unique method of deliberate self-induction of epileptic seizures, using the TV screen was encountered in two young women. Both suffered from highly photosensitive primary generalized corticoreticular orcentrencephalic epilepsy. Self-precipitation developed in periods of their life when spontaneous attacks no longer occurred. The seizures consisted of absence or generalized tonic clonic attacks. (The latter are almost never encountered in \"hand waving\", the most common form of self-induction reported in the literature.) The seizures were almost always induced in situations of guilt and/or frustation. They were followed by a feeling of tension relief rather than pleasure. Both patients were reluctant to discuss the circumstances under which self-induction occurred, and had difficulty in accepting psychotherapy or complying with antiepileptic medication. The similarity between the two young women was striking, and suggests that patients with this and other forms of self-induced epilepsy should be more fully studied from a psychiatric point of view.", "contents": "SELF-INDUCED TELEVISION EPILEPSY. A study of 2 patients. A unique method of deliberate self-induction of epileptic seizures, using the TV screen was encountered in two young women. Both suffered from highly photosensitive primary generalized corticoreticular orcentrencephalic epilepsy. Self-precipitation developed in periods of their life when spontaneous attacks no longer occurred. The seizures consisted of absence or generalized tonic clonic attacks. (The latter are almost never encountered in \"hand waving\", the most common form of self-induction reported in the literature.) The seizures were almost always induced in situations of guilt and/or frustation. They were followed by a feeling of tension relief rather than pleasure. Both patients were reluctant to discuss the circumstances under which self-induction occurred, and had difficulty in accepting psychotherapy or complying with antiepileptic medication. The similarity between the two young women was striking, and suggests that patients with this and other forms of self-induced epilepsy should be more fully studied from a psychiatric point of view.", "PMID": 953952}
{"id": "pubmed23n0032_798", "title": "The oedipus complex. A critical re-examination.", "content": "The Oedipus complex no longer holds the crucial role in psychodynamics attributed to it by Freud. Attempts to distinguish between oedipal and pre-oedipal disturbances are brought into question, and it is suggested that the complex reflects many non-psychosexual issues, particularly separation-individuation. Ten views of the Oedipus complex are critically reviewed.", "contents": "The oedipus complex. A critical re-examination. The Oedipus complex no longer holds the crucial role in psychodynamics attributed to it by Freud. Attempts to distinguish between oedipal and pre-oedipal disturbances are brought into question, and it is suggested that the complex reflects many non-psychosexual issues, particularly separation-individuation. Ten views of the Oedipus complex are critically reviewed.", "PMID": 953954}
{"id": "pubmed23n0032_799", "title": "Treatment of advanced malignancy with CCNU (NSC 79037): a phase II cooperative study with long-term follow up.", "content": "CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea, NSC-79037) was used to treat advanced malignancies in 329 evaluable patients. The treatment dosage was 130 mg/m2 for patients with adequate bone marrow reserve and 100 mg/m2 for those with compromised bone marrow. Oral treatment was repeated at 6-week intervals unless hematologic toxicity intervened. There were four complete responses: two in ovarian cancer, one with small cell carcinoma of the lung, and one with melanoma. Tumor response greater than 50% reduction in tumor size occurred in 39 patients (11.9%) while stable disease (no change or decrease or increase of less than 50% in tumor size) was noted in 152 patients (46.2%). Tumor progression occurred in 130 cases. Melanomas and ovarian and lung cancers had the highest response rates. Bone marrow depression was the major side effect of treatment; there was a significant positive correlation between the severity of leukopenia and thrombocytopenia and tumor response to treatment.", "contents": "Treatment of advanced malignancy with CCNU (NSC 79037): a phase II cooperative study with long-term follow up. CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea, NSC-79037) was used to treat advanced malignancies in 329 evaluable patients. The treatment dosage was 130 mg/m2 for patients with adequate bone marrow reserve and 100 mg/m2 for those with compromised bone marrow. Oral treatment was repeated at 6-week intervals unless hematologic toxicity intervened. There were four complete responses: two in ovarian cancer, one with small cell carcinoma of the lung, and one with melanoma. Tumor response greater than 50% reduction in tumor size occurred in 39 patients (11.9%) while stable disease (no change or decrease or increase of less than 50% in tumor size) was noted in 152 patients (46.2%). Tumor progression occurred in 130 cases. Melanomas and ovarian and lung cancers had the highest response rates. Bone marrow depression was the major side effect of treatment; there was a significant positive correlation between the severity of leukopenia and thrombocytopenia and tumor response to treatment.", "PMID": 953955}
{"id": "pubmed23n0032_800", "title": "Methyl-CCNU, alone and in combination with vincristine, or vincristine and methotrexate, in advanced bronchogenic carcinoma.", "content": "Ninety-two patients with advanced bronchogenic carcinoma were prospectively randomized according to performance status, histology, and extent of disease to methyl-CCNU alone; methyl-CCNU and vincristine; or methyl-CCNU, vincristine, and methotrexate. Seventy-three patients were evaluable. Randomization to methyl-CCNU alone was discontinued when only three brief \"static\" responses were noted in 11 patients and the survival (p less than .01) and time on study (p less than .05) were noted to be significantly less than with the combination. Two of 32 patients treated with methyl-CCNU and vincristine, and two of 30 patients treated with methyl-CCNU, vincristine, and methotrexate had mixed responses with a median of 67.5 days.  A static response was seen in eight of 32 and none of 30 patients, respectively. Minimal toxicity occurred in all regimens. Methyl-CCNU alone or in combination with vincristine or vincristine and methotrexate is of limited benefit in patients with lung cancer, although our data suggest (p = 0.15) that the addition of methotrexate increases time on study and survival in patients with extensive disease.", "contents": "Methyl-CCNU, alone and in combination with vincristine, or vincristine and methotrexate, in advanced bronchogenic carcinoma. Ninety-two patients with advanced bronchogenic carcinoma were prospectively randomized according to performance status, histology, and extent of disease to methyl-CCNU alone; methyl-CCNU and vincristine; or methyl-CCNU, vincristine, and methotrexate. Seventy-three patients were evaluable. Randomization to methyl-CCNU alone was discontinued when only three brief \"static\" responses were noted in 11 patients and the survival (p less than .01) and time on study (p less than .05) were noted to be significantly less than with the combination. Two of 32 patients treated with methyl-CCNU and vincristine, and two of 30 patients treated with methyl-CCNU, vincristine, and methotrexate had mixed responses with a median of 67.5 days.  A static response was seen in eight of 32 and none of 30 patients, respectively. Minimal toxicity occurred in all regimens. Methyl-CCNU alone or in combination with vincristine or vincristine and methotrexate is of limited benefit in patients with lung cancer, although our data suggest (p = 0.15) that the addition of methotrexate increases time on study and survival in patients with extensive disease.", "PMID": 953956}
{"id": "pubmed23n0032_801", "title": "Malignant histiocytosis: complete remission with combination chemotherapy.", "content": "A case of a 16-year-old girl with malignant histiocytosis involving the lymph nodes, bone marrow, peripheral blood, liver, and spleen is described. This patient achieved a complete remission with cyclic quadruple chemotherapy consisting of cyclophosphamide, vincristine, procarbazine, and prednisone (COPP). She is alive and free of disease more than 4 years after the completion of COPP and after having had maintenance treatment with a small dose of cyclophosphamide for 2 3/4 years. Such good results with the COPP regimen in malignant histiocytosis have not been reported previously, and it is suggested that this modality of treatment warrants further trial.", "contents": "Malignant histiocytosis: complete remission with combination chemotherapy. A case of a 16-year-old girl with malignant histiocytosis involving the lymph nodes, bone marrow, peripheral blood, liver, and spleen is described. This patient achieved a complete remission with cyclic quadruple chemotherapy consisting of cyclophosphamide, vincristine, procarbazine, and prednisone (COPP). She is alive and free of disease more than 4 years after the completion of COPP and after having had maintenance treatment with a small dose of cyclophosphamide for 2 3/4 years. Such good results with the COPP regimen in malignant histiocytosis have not been reported previously, and it is suggested that this modality of treatment warrants further trial.", "PMID": 953957}
{"id": "pubmed23n0032_802", "title": "Skin ulcers due to adriamycin.", "content": "Local skin necrosis at the site of intravenous or intra-arterial adriamycin infusion is an infrequent, but serious complication. Ulcers secondary to adriamycin have insidious beginnings, but progress to a much deeper extent than would be expected from their initial appearance. Deep structures, such as tendon or bone, may become exposed. The ulcers are indolent and do not develop a granulation tissue response or epithelialization, as might be expected from their early appearance. Injections of adriamycin in the dorsum of the hand should be avoided when possible, since tendons have little skin cover and the area is difficult to cover with local tissue if there is skin loss. While prevention is important, early surgical treatment may prevent progressive deep involvement and seems warranted when the patient has a life expectancy of months or years. Wide excision of all inflamed tissue is the treatment of choice, with split-thickness skin grafting or flap coverage.", "contents": "Skin ulcers due to adriamycin. Local skin necrosis at the site of intravenous or intra-arterial adriamycin infusion is an infrequent, but serious complication. Ulcers secondary to adriamycin have insidious beginnings, but progress to a much deeper extent than would be expected from their initial appearance. Deep structures, such as tendon or bone, may become exposed. The ulcers are indolent and do not develop a granulation tissue response or epithelialization, as might be expected from their early appearance. Injections of adriamycin in the dorsum of the hand should be avoided when possible, since tendons have little skin cover and the area is difficult to cover with local tissue if there is skin loss. While prevention is important, early surgical treatment may prevent progressive deep involvement and seems warranted when the patient has a life expectancy of months or years. Wide excision of all inflamed tissue is the treatment of choice, with split-thickness skin grafting or flap coverage.", "PMID": 953958}
{"id": "pubmed23n0032_803", "title": "Single view mammography: a simple and efficent approach to breast cancer screening.", "content": "In 1974 a new approach to breast cancer screening was introduced--the single view mammography method. An urban nonselected population of 6845 women of more than 34 years of age were screened with one roentgenogram of each breast. By this method it has been possible to reduce the radiation dose, while the patient flow is increased and the costs are decreased to an acceptable level. Forty cases of breast carcinoma were detected (6.7/1000). Twenty cases were preclinical.", "contents": "Single view mammography: a simple and efficent approach to breast cancer screening. In 1974 a new approach to breast cancer screening was introduced--the single view mammography method. An urban nonselected population of 6845 women of more than 34 years of age were screened with one roentgenogram of each breast. By this method it has been possible to reduce the radiation dose, while the patient flow is increased and the costs are decreased to an acceptable level. Forty cases of breast carcinoma were detected (6.7/1000). Twenty cases were preclinical.", "PMID": 953959}
{"id": "pubmed23n0032_804", "title": "Routine full-lung tomography in the initial staging and treatment planning of patients with Hodgkin's disease and non-Hodgkin's lymphoma.", "content": "The yield of additional information from anteroposterior full-lung tomograms that changed stage or treatment, in comparison to that obtained from routine chest radiographs, was prospectively evaluated in 243 previously untreated patients with Hodgkin's disease and non-Hodgkin's lymphoma. Although new information was found in 21.4% of all patients, in only 1.2% did these additional data change patient staging. In 3.3% of the other patients the tomograms provided information that affected radiotherapy treatment planning as practiced in our institution.", "contents": "Routine full-lung tomography in the initial staging and treatment planning of patients with Hodgkin's disease and non-Hodgkin's lymphoma. The yield of additional information from anteroposterior full-lung tomograms that changed stage or treatment, in comparison to that obtained from routine chest radiographs, was prospectively evaluated in 243 previously untreated patients with Hodgkin's disease and non-Hodgkin's lymphoma. Although new information was found in 21.4% of all patients, in only 1.2% did these additional data change patient staging. In 3.3% of the other patients the tomograms provided information that affected radiotherapy treatment planning as practiced in our institution.", "PMID": 953960}
{"id": "pubmed23n0032_805", "title": "The value of the liver scan in preoperative screening of patients with malignancies.", "content": "This study analyzes the value of the liver scan as a preoperative screening procedure for occult liver metastases in patients with melanoma, sarcoma, head and neck carcinoma, and pelvic carcinoma. The records of 566 consecutive patients admitted to the Surgery Branch of the National Cancer Institute between 1969 and 1974 were reviewed and 323 patients were found acceptable for inclusion in the study. In these patients, although the liver scan had an overall accuracy of 95%, the scan identified only 50% of the patients with occult metastases to the liver and did not siginificantly add to the yield of the other screening procedures. It was useful as an adjuvant to an abnormal routine workup to confirm and localize metastases to the liver. Scans with only non specific abnormalities were of little help. Liver metastases were not identified in any patients with sarcoma, head and neck cancer, or clinically localized carcinoma of the cervix. Therefore, the liver scan was determimed to be an unnecessary part of their screening workup. Patients with recurrent or advanced carcinoma of the cervix and advanced melanoma were found to have an increased incidence of liver metastases. There was a 10% incidence of occult metastases to liver in patients with melanoma and the incidence increased with advancing clinical stage of disease. Even in this high-risk group of patients the screening liver scan did not significantly add information to that gained by history, physical examination, and blood work.", "contents": "The value of the liver scan in preoperative screening of patients with malignancies. This study analyzes the value of the liver scan as a preoperative screening procedure for occult liver metastases in patients with melanoma, sarcoma, head and neck carcinoma, and pelvic carcinoma. The records of 566 consecutive patients admitted to the Surgery Branch of the National Cancer Institute between 1969 and 1974 were reviewed and 323 patients were found acceptable for inclusion in the study. In these patients, although the liver scan had an overall accuracy of 95%, the scan identified only 50% of the patients with occult metastases to the liver and did not siginificantly add to the yield of the other screening procedures. It was useful as an adjuvant to an abnormal routine workup to confirm and localize metastases to the liver. Scans with only non specific abnormalities were of little help. Liver metastases were not identified in any patients with sarcoma, head and neck cancer, or clinically localized carcinoma of the cervix. Therefore, the liver scan was determimed to be an unnecessary part of their screening workup. Patients with recurrent or advanced carcinoma of the cervix and advanced melanoma were found to have an increased incidence of liver metastases. There was a 10% incidence of occult metastases to liver in patients with melanoma and the incidence increased with advancing clinical stage of disease. Even in this high-risk group of patients the screening liver scan did not significantly add information to that gained by history, physical examination, and blood work.", "PMID": 953961}
{"id": "pubmed23n0032_806", "title": "Bone scanning in lymphoma.", "content": "The results of bone scanning with the newer technetium-99m complexes were correlated with clinical, laboratory, and radiographic findings in 26 patients with malignant lymphoma (10 with Hodgkin's disease and 16 with non-Hodgkin's lymphomas). Abnormalities on bone scan compatible with lymphomatous involvement of the skeleton appeared to occur more commonly in patients with diffuse lymphomas than in patients with nodular lymphomas and were generally observed in the setting of advanced disease (15 of 23 patients). Twenty-seven (73%) of the 37 scans obtained were abnormal. Although abnormal scans were observed with the greatest frequency in patients with bone pain (11 of 11), bone marrow involvement (11 of 12), abnormal skeletal radiographs (11 of 11), and elevated serum alkaline phosphatase levels (5 of 6), bone scanning also detected lymphomatous involvement in patients free of pain or with normal laboratory tests. Moreover, conventional radiography was entirely normal in six (35%) of 17 patients with abnormal scans and revealed only nonspecific osteopenia in another two patients (12%). Serial bone scans in nine patients reflected their response to chemotherapy. Of the 37 scans, only one was judged falsely positive and one falsey negative. Bone scanning with 99mTc complexes is a safe, simple, and sensitive screening procedure for detecting both extensive and focal lymphomatous involvement of the skeletal system and is a useful means of following such involvement in response to treatment.", "contents": "Bone scanning in lymphoma. The results of bone scanning with the newer technetium-99m complexes were correlated with clinical, laboratory, and radiographic findings in 26 patients with malignant lymphoma (10 with Hodgkin's disease and 16 with non-Hodgkin's lymphomas). Abnormalities on bone scan compatible with lymphomatous involvement of the skeleton appeared to occur more commonly in patients with diffuse lymphomas than in patients with nodular lymphomas and were generally observed in the setting of advanced disease (15 of 23 patients). Twenty-seven (73%) of the 37 scans obtained were abnormal. Although abnormal scans were observed with the greatest frequency in patients with bone pain (11 of 11), bone marrow involvement (11 of 12), abnormal skeletal radiographs (11 of 11), and elevated serum alkaline phosphatase levels (5 of 6), bone scanning also detected lymphomatous involvement in patients free of pain or with normal laboratory tests. Moreover, conventional radiography was entirely normal in six (35%) of 17 patients with abnormal scans and revealed only nonspecific osteopenia in another two patients (12%). Serial bone scans in nine patients reflected their response to chemotherapy. Of the 37 scans, only one was judged falsely positive and one falsey negative. Bone scanning with 99mTc complexes is a safe, simple, and sensitive screening procedure for detecting both extensive and focal lymphomatous involvement of the skeletal system and is a useful means of following such involvement in response to treatment.", "PMID": 953962}
{"id": "pubmed23n0032_807", "title": "Immunocompetence, immunosuppression, and human breast cancer. I. An analysis of their relationship by known parameters of cell-mediated immunity in well-defined clinical stages of disease.", "content": "General immune competence was examined 255 breast cancer patients, including 104 operable, 44 locally advanced/inoperable, and 44 with demonstrable metastatic dissemination, all at the time of diagnosis, as well as 63 disease-free long survivors; this was compared with that of 100 normal controls. The parameters employed were PPD and DNCB skin testing, lymphocyte response to PHA mitogen, E-rosette formation, and lymphocyte number. Significant patients, with only 31% showing optimal and 25% showing minimal levels of immune function, as compared with 70% optimal and 2% minimal function in controls. Immune competence was not affected by metastatic involvement of regional lymph nodes. In patients with early, occult metastatic dissemination (as determined in retrospect), the degree of immune competence was found to be identical to that of patients who did not develop disease dissemination. Remarkably, this early phase of tumor spread is not accompanied by immune impairment, such as is evident in clinically demonstrable metastatic disease and, to a lesser degree, in advanced local and regional disease. Since tumor dissemination preceded impairment of general immunocompetence, it emerges as the cause rather than the result of immunosuppression. Long disease-free survivors, who had postoperative irradiation 5-12 years previously, were shown to have a notably low level of immune competence. Lymphocyte response to PHA stimulation was found to be impaired in the earlier stages of disease, while skin DHR was still well maintained; in advanced disease both parameters tend to correlate as total immunologic impairment ensues. The sequence of immunologic events leading up to immunosuppression with disease progress is discussed.", "contents": "Immunocompetence, immunosuppression, and human breast cancer. I. An analysis of their relationship by known parameters of cell-mediated immunity in well-defined clinical stages of disease. General immune competence was examined 255 breast cancer patients, including 104 operable, 44 locally advanced/inoperable, and 44 with demonstrable metastatic dissemination, all at the time of diagnosis, as well as 63 disease-free long survivors; this was compared with that of 100 normal controls. The parameters employed were PPD and DNCB skin testing, lymphocyte response to PHA mitogen, E-rosette formation, and lymphocyte number. Significant patients, with only 31% showing optimal and 25% showing minimal levels of immune function, as compared with 70% optimal and 2% minimal function in controls. Immune competence was not affected by metastatic involvement of regional lymph nodes. In patients with early, occult metastatic dissemination (as determined in retrospect), the degree of immune competence was found to be identical to that of patients who did not develop disease dissemination. Remarkably, this early phase of tumor spread is not accompanied by immune impairment, such as is evident in clinically demonstrable metastatic disease and, to a lesser degree, in advanced local and regional disease. Since tumor dissemination preceded impairment of general immunocompetence, it emerges as the cause rather than the result of immunosuppression. Long disease-free survivors, who had postoperative irradiation 5-12 years previously, were shown to have a notably low level of immune competence. Lymphocyte response to PHA stimulation was found to be impaired in the earlier stages of disease, while skin DHR was still well maintained; in advanced disease both parameters tend to correlate as total immunologic impairment ensues. The sequence of immunologic events leading up to immunosuppression with disease progress is discussed.", "PMID": 953963}
{"id": "pubmed23n0032_808", "title": "Multiple pulmonary leiomyomatous hamartomas: report of a case with ultrastructure examination.", "content": "The electron microscopic features of pulmonary leiomyomatous hamartoma, very rare neoplasm, were studied. Characteristic smooth muscle cells containing microfilaments, dense bodies, plaques, pinocytotic vesicles, and basal lamina were present. Fibrosis was minimal, which supports the belief that this component is a secondary phenomena.", "contents": "Multiple pulmonary leiomyomatous hamartomas: report of a case with ultrastructure examination. The electron microscopic features of pulmonary leiomyomatous hamartoma, very rare neoplasm, were studied. Characteristic smooth muscle cells containing microfilaments, dense bodies, plaques, pinocytotic vesicles, and basal lamina were present. Fibrosis was minimal, which supports the belief that this component is a secondary phenomena.", "PMID": 953964}
{"id": "pubmed23n0032_809", "title": "On the nature of desmoplastic melanoma.", "content": "Pathologic study demonstrated lentigo maligna melanoma in three of four cases of desmoplastic melanoma and demonstrated spindle cell masses with features of atypical fibroxanthoma in all four of the cases. Pigment enzymes of melanosomes were not found in the mesenchymal portion of the tumor. The cellular atypia and the histochemical and ultrastructural findings favor the fibroblastic nature of this melanoma-related dermal mass.", "contents": "On the nature of desmoplastic melanoma. Pathologic study demonstrated lentigo maligna melanoma in three of four cases of desmoplastic melanoma and demonstrated spindle cell masses with features of atypical fibroxanthoma in all four of the cases. Pigment enzymes of melanosomes were not found in the mesenchymal portion of the tumor. The cellular atypia and the histochemical and ultrastructural findings favor the fibroblastic nature of this melanoma-related dermal mass.", "PMID": 953965}
{"id": "pubmed23n0032_810", "title": "Siderosis of lymph nodes patients with Hodgkin's disease.", "content": "Lymph nodes removed from 28 untreated patients with Hodgkin's disease all contained markedly increased amounts of hemosiderine, whether or not they were histologically involved in the disease. This finding was particularly striking in patients with the nodular sclerosis type of disease. Abnormal deposits of iron were also noted frequently in lymph nodes containing metastatic carcinoma, lymphoma of non-Hodgkin's type, and reactive hyperplasia, but in each case, with the exception of metastatic squamous cell carcinoma, the amount was significantly less than seen in Hodgkin's disease. The findings suggest that in patients with Hodgkin's disease and perhaps in those with other disorders in which abnormal tissue retention of iron underlies sideropenic anemia, lymph nodes are an important site of iron retention.", "contents": "Siderosis of lymph nodes patients with Hodgkin's disease. Lymph nodes removed from 28 untreated patients with Hodgkin's disease all contained markedly increased amounts of hemosiderine, whether or not they were histologically involved in the disease. This finding was particularly striking in patients with the nodular sclerosis type of disease. Abnormal deposits of iron were also noted frequently in lymph nodes containing metastatic carcinoma, lymphoma of non-Hodgkin's type, and reactive hyperplasia, but in each case, with the exception of metastatic squamous cell carcinoma, the amount was significantly less than seen in Hodgkin's disease. The findings suggest that in patients with Hodgkin's disease and perhaps in those with other disorders in which abnormal tissue retention of iron underlies sideropenic anemia, lymph nodes are an important site of iron retention.", "PMID": 953966}
{"id": "pubmed23n0032_811", "title": "Effects of human and experimental cancer on the conversion of 14C tripalmitin to 14CO2.", "content": "14C Tripalmitin was administered intraperitoneally to control mice and to mice bearing subcutaneous Krebs-2 carcinoma. The rate of excretion of 14CO2 was studied. The presence of a tumor induced a greater than 80% suppression of the formation of 14CO2 within 24 hours from the implantation of the tumor, thus allowing unambiguous identification of the tumor-bearing mice. When cancer patients were studied, along with normal volunteers and patients with non-neoplastic diseases, 14CO2 excretion data could be used to identify the cancer patients with an overall accuracy of 84%.", "contents": "Effects of human and experimental cancer on the conversion of 14C tripalmitin to 14CO2. 14C Tripalmitin was administered intraperitoneally to control mice and to mice bearing subcutaneous Krebs-2 carcinoma. The rate of excretion of 14CO2 was studied. The presence of a tumor induced a greater than 80% suppression of the formation of 14CO2 within 24 hours from the implantation of the tumor, thus allowing unambiguous identification of the tumor-bearing mice. When cancer patients were studied, along with normal volunteers and patients with non-neoplastic diseases, 14CO2 excretion data could be used to identify the cancer patients with an overall accuracy of 84%.", "PMID": 953967}
{"id": "pubmed23n0032_812", "title": "Effects of nitrogen mustard and splenectomy on mouse bone marrow colony formation in vitro.", "content": "Improved tolerance of splenectomized patients with Hodgkin's disease (HD) to radiotherapy and chemotherapy has been reported. The present study was undertaken to determine the effects of splenectomy and nitrogen mustard (NM) on colony-forming cells (CFC's) of bone marrow cells obtained from CF1 male mice by in bitro agar-gel technique. Splenectomized mice were given NM intraperitoneally on day 11. On day 15, they were sacrificed and the bone marrow was cultured with a source of colony-stimulating factor (CSF). Spleen extract was prepared by grinding spleens from CF1 mice. On the eighth day of incubation, significantly higher numbers of CFC's were found in splenectomized animals at 1% confidence level (F Test) compared with the nonsplenectomized animals. Both splenectomized and non-splenectomized mice had a greater colony response after NM (at 5% confidence level) than saline-treated controls. Maximum numbers of colonies were obtained in the nustard-treated asplenic animals. Splenic extract, as well as extracts from other organs, when added to the culture plates resulted in inhibition of colony formation. The significance of in vitro inhibition after addition of organ extract is uncertain.", "contents": "Effects of nitrogen mustard and splenectomy on mouse bone marrow colony formation in vitro. Improved tolerance of splenectomized patients with Hodgkin's disease (HD) to radiotherapy and chemotherapy has been reported. The present study was undertaken to determine the effects of splenectomy and nitrogen mustard (NM) on colony-forming cells (CFC's) of bone marrow cells obtained from CF1 male mice by in bitro agar-gel technique. Splenectomized mice were given NM intraperitoneally on day 11. On day 15, they were sacrificed and the bone marrow was cultured with a source of colony-stimulating factor (CSF). Spleen extract was prepared by grinding spleens from CF1 mice. On the eighth day of incubation, significantly higher numbers of CFC's were found in splenectomized animals at 1% confidence level (F Test) compared with the nonsplenectomized animals. Both splenectomized and non-splenectomized mice had a greater colony response after NM (at 5% confidence level) than saline-treated controls. Maximum numbers of colonies were obtained in the nustard-treated asplenic animals. Splenic extract, as well as extracts from other organs, when added to the culture plates resulted in inhibition of colony formation. The significance of in vitro inhibition after addition of organ extract is uncertain.", "PMID": 953968}
{"id": "pubmed23n0032_813", "title": "Blastic phase of agnogenic myeloid metaplasia simulating malignant lymphoma.", "content": "A patient with angogenic myeloid metaplasia in blastic transformation with unusually prominent lymphadenopathy simulating lymphoma is described. Interestingly, the initial evidence of myeloblastic transformation was present in the lymph nodes but not in the marrow. Serial bone marrow biopsies showed fibrosis throughout the course of the disease, in spite of a gradual increase in the percentage of peripheral blood myeloblasts. Aneuploidy with a marker chromosome and a Philadelphia-like chromosome was present in the lymph node, bone marrow, and peripheral blood cells. The literature is reviewed regarding the incidence of prominent lymphadenopathy and chromosome abnormalities in agnogenic myeloid metaplasia and other myeloproliferative disorders.", "contents": "Blastic phase of agnogenic myeloid metaplasia simulating malignant lymphoma. A patient with angogenic myeloid metaplasia in blastic transformation with unusually prominent lymphadenopathy simulating lymphoma is described. Interestingly, the initial evidence of myeloblastic transformation was present in the lymph nodes but not in the marrow. Serial bone marrow biopsies showed fibrosis throughout the course of the disease, in spite of a gradual increase in the percentage of peripheral blood myeloblasts. Aneuploidy with a marker chromosome and a Philadelphia-like chromosome was present in the lymph node, bone marrow, and peripheral blood cells. The literature is reviewed regarding the incidence of prominent lymphadenopathy and chromosome abnormalities in agnogenic myeloid metaplasia and other myeloproliferative disorders.", "PMID": 953969}
{"id": "pubmed23n0032_814", "title": "Oncocytic neoplasms of salivary glands: a report of fifteen cases including two malignant oncocytomas.", "content": "Fifteen oncocytic (oxyphilic granular cell) neoplasms of the salivary glands collected from three large university medical centers are presented. The histologic and ultrastructural criteria that characterize oncocytes are presented and electron micrographs of two of our cases are discussed. Included in the series are 10 benign oncocytomas, two malignant oncocytomas and three benign, oncocytic pleomorphic adenomas. The clinical and pathologic features of benign oncocytomas are reviewed, with special attention to the variety of gross and microscopic changes present. The literature on malignant oncocytomas is critically reviewed, diagnostic criteria are evaluated, and a unique case is discussed in detail. Reference is made to a group of neoplasms that we designate as benign locally aggressive oncocytomas. The problem of differential diagnosis between oncocytoma and bening, oncocytic pleomorphic adenoma is considered.", "contents": "Oncocytic neoplasms of salivary glands: a report of fifteen cases including two malignant oncocytomas. Fifteen oncocytic (oxyphilic granular cell) neoplasms of the salivary glands collected from three large university medical centers are presented. The histologic and ultrastructural criteria that characterize oncocytes are presented and electron micrographs of two of our cases are discussed. Included in the series are 10 benign oncocytomas, two malignant oncocytomas and three benign, oncocytic pleomorphic adenomas. The clinical and pathologic features of benign oncocytomas are reviewed, with special attention to the variety of gross and microscopic changes present. The literature on malignant oncocytomas is critically reviewed, diagnostic criteria are evaluated, and a unique case is discussed in detail. Reference is made to a group of neoplasms that we designate as benign locally aggressive oncocytomas. The problem of differential diagnosis between oncocytoma and bening, oncocytic pleomorphic adenoma is considered.", "PMID": 953970}
{"id": "pubmed23n0032_815", "title": "Carcinoma metastatic to the eye and orbit III. A clinicopathologic study of 28 cases metastatic to the orbit.", "content": "Two hundred and twenty-seven cases of carcinoma metastatic to the eye and orbit have been reviewed previously. The orbit alone was involved in 28 cases: theses lesions constitute the basis of this clinicopathologic study. The most common signs and symptoms produced by orbital metastasis included exophthalmos (75%1, pain (29%), decreased vision (29%), periorbital swelling (25%), a visible mass (21%), ophthalmoplegia, and diplopia (18%). The ophthalmologists' preoperative (or premortem) clinical diagnoses were: orbital mass, 36%; metastatic carcinoma, 29%; leukemia, 7%; melanosarcoma, 4%; mixed tumor of lacrimal gland, 4%; and meningioma, 4%. The sites of the primary tumors in the 28 patients with orbital metastasis were as follows: breast, eight; lung, four; genitourinary tract, four; pancreas, one; and ileum, one. In 10 patients the site of the primary carcinoma was not determined. In 17 of the 28 patients, symptoms of orbital metastasis preceded detection of a primary tumor elsewhere in the body. In 10 of the remaining 11 patients, detection of the primary tumor had preceded the onset of orbital symptoms. In one patient, symptoms of the primary tumor and of orbital metastasis appeared at about the same time. The median survival of patients with carcinoma metastatic to the orbit was 15.6 months from the time of orbital surgery. This was much better than the median survival of the 227 patients in the overall study (7.4 months) and far better than the median survival of the patients with metastasis to the anterior segment of the eye (only 5.4 months).", "contents": "Carcinoma metastatic to the eye and orbit III. A clinicopathologic study of 28 cases metastatic to the orbit. Two hundred and twenty-seven cases of carcinoma metastatic to the eye and orbit have been reviewed previously. The orbit alone was involved in 28 cases: theses lesions constitute the basis of this clinicopathologic study. The most common signs and symptoms produced by orbital metastasis included exophthalmos (75%1, pain (29%), decreased vision (29%), periorbital swelling (25%), a visible mass (21%), ophthalmoplegia, and diplopia (18%). The ophthalmologists' preoperative (or premortem) clinical diagnoses were: orbital mass, 36%; metastatic carcinoma, 29%; leukemia, 7%; melanosarcoma, 4%; mixed tumor of lacrimal gland, 4%; and meningioma, 4%. The sites of the primary tumors in the 28 patients with orbital metastasis were as follows: breast, eight; lung, four; genitourinary tract, four; pancreas, one; and ileum, one. In 10 patients the site of the primary carcinoma was not determined. In 17 of the 28 patients, symptoms of orbital metastasis preceded detection of a primary tumor elsewhere in the body. In 10 of the remaining 11 patients, detection of the primary tumor had preceded the onset of orbital symptoms. In one patient, symptoms of the primary tumor and of orbital metastasis appeared at about the same time. The median survival of patients with carcinoma metastatic to the orbit was 15.6 months from the time of orbital surgery. This was much better than the median survival of the 227 patients in the overall study (7.4 months) and far better than the median survival of the patients with metastasis to the anterior segment of the eye (only 5.4 months).", "PMID": 953971}
{"id": "pubmed23n0032_816", "title": "Urinary polyamine levels in patients with localized malignancy.", "content": "Polyamine levels (putrescine, spermidine, and spermine) were determined in 24-hour urine samples by a high voltage electroporesis techique. Twenty-four of 26 patients with localized malignant tumors had two or more elevated urinary polyamine levels. Seven of 12 patients with regional spread of their cancer and five of 11 patients with localized benign and/or noninvasive tumors had elevated urinary polyamine levels. Elevations were seen more frequently frequently in patients with gynecologic tumors. Our data suggest that there is no significant difference between the individual of total polyamine levels obtained in patients with localized malignant tumors, and those levels obtained in patients previously studied with widespread metastatic disease.", "contents": "Urinary polyamine levels in patients with localized malignancy. Polyamine levels (putrescine, spermidine, and spermine) were determined in 24-hour urine samples by a high voltage electroporesis techique. Twenty-four of 26 patients with localized malignant tumors had two or more elevated urinary polyamine levels. Seven of 12 patients with regional spread of their cancer and five of 11 patients with localized benign and/or noninvasive tumors had elevated urinary polyamine levels. Elevations were seen more frequently frequently in patients with gynecologic tumors. Our data suggest that there is no significant difference between the individual of total polyamine levels obtained in patients with localized malignant tumors, and those levels obtained in patients previously studied with widespread metastatic disease.", "PMID": 953972}
{"id": "pubmed23n0032_817", "title": "Cervical cancer control: a study of morbidity and mortality trends over a twenty-one-year period.", "content": "Data from a 21-year period are presented to evaluate the effects of a mass cytologic screening program on uterine cancer morbidity and mortality in Louisville, Jefferson County, Kentucky. The success of screening was greatest in the younger age groups. There was a fall-off after age 45 years, especially in those age 60 years or older. Women at highest risk for cervical cancer, in the low socioeconomic quartile, had a better initial screening rate than the two middle-income quartiles, and had the highest rate of all women for subsequent rescreening. The greatest decrease in both morbidity and mortality was in women under the age of 50 years. Women age 30-39 and 50-59 years benefitted the most, as measured by mortality, with a decrease of 70.8 and 69.0%, respectively. There was no change in mortality rates for those age 70 years or older. Although the average annual age-adjusted rate increased from 13.2 to 15.3/100,000 women over the 21-year period.", "contents": "Cervical cancer control: a study of morbidity and mortality trends over a twenty-one-year period. Data from a 21-year period are presented to evaluate the effects of a mass cytologic screening program on uterine cancer morbidity and mortality in Louisville, Jefferson County, Kentucky. The success of screening was greatest in the younger age groups. There was a fall-off after age 45 years, especially in those age 60 years or older. Women at highest risk for cervical cancer, in the low socioeconomic quartile, had a better initial screening rate than the two middle-income quartiles, and had the highest rate of all women for subsequent rescreening. The greatest decrease in both morbidity and mortality was in women under the age of 50 years. Women age 30-39 and 50-59 years benefitted the most, as measured by mortality, with a decrease of 70.8 and 69.0%, respectively. There was no change in mortality rates for those age 70 years or older. Although the average annual age-adjusted rate increased from 13.2 to 15.3/100,000 women over the 21-year period.", "PMID": 953973}
{"id": "pubmed23n0032_818", "title": "Population cancer screening.", "content": "Controlled trials to evaluate mass screening programs for cancer detection have been singularly lacking. High cost, lack of medical manpower, and low yield have contributed to this problem. A new program in cancer detection (CANSCREEN) has been developed jointly by The Fox Chase Cancer Center in Philadelphia and the Preventive Medicine Institute in New York City. This program attempts to provide a quality cancer-detection examination with increased cost effectiveness. Features include: 1) a self-administered questionnaire on medical history, symptoms, and risk factors; 2) nonphysican examiners; 3) risk-facotr analysis with a predetermined decision logic to determine type and periodicity of examination; and 4) primary intervention (health education). This collaborative program between two institutions in two cities demonstrates the feasibility of introducing similar programs elsewhere. A data base shared by cooperating centers permits information on all patients to be used for evaluation of new techniques, end results, etc. A randomized controlled trial has been designed to evaluate the effectiveness and efficiency of the questionnaire alone and of the questionnaire and examination.", "contents": "Population cancer screening. Controlled trials to evaluate mass screening programs for cancer detection have been singularly lacking. High cost, lack of medical manpower, and low yield have contributed to this problem. A new program in cancer detection (CANSCREEN) has been developed jointly by The Fox Chase Cancer Center in Philadelphia and the Preventive Medicine Institute in New York City. This program attempts to provide a quality cancer-detection examination with increased cost effectiveness. Features include: 1) a self-administered questionnaire on medical history, symptoms, and risk factors; 2) nonphysican examiners; 3) risk-facotr analysis with a predetermined decision logic to determine type and periodicity of examination; and 4) primary intervention (health education). This collaborative program between two institutions in two cities demonstrates the feasibility of introducing similar programs elsewhere. A data base shared by cooperating centers permits information on all patients to be used for evaluation of new techniques, end results, etc. A randomized controlled trial has been designed to evaluate the effectiveness and efficiency of the questionnaire alone and of the questionnaire and examination.", "PMID": 953974}
{"id": "pubmed23n0032_819", "title": "Parathyroid adenoma and nonmedullary thyroid carcinoma.", "content": "The incidence of thyroid carcinoma in patients with hyperparathyroidism has been reported as higher than expected. We record our experience with 272 cases of primary hyperparathyroidism. Malignant thyroid lesions were found in 11.4%. We conclude that this is more than a fortuitous association.", "contents": "Parathyroid adenoma and nonmedullary thyroid carcinoma. The incidence of thyroid carcinoma in patients with hyperparathyroidism has been reported as higher than expected. We record our experience with 272 cases of primary hyperparathyroidism. Malignant thyroid lesions were found in 11.4%. We conclude that this is more than a fortuitous association.", "PMID": 953975}
{"id": "pubmed23n0032_820", "title": "Melanomas of the lower extremity among native Puerto Ricans.", "content": "The clinical features of 57 melanomas of the lower extremity occurring in native Puerto Ricans were studied. Whereas most melanomas of the lower extremity among Caucasians occur above the ankle, the group studied showed a predilection for the foot, particularly in the minimally pigmented zones (sole, heel, and nail bed), a distribution similar to that reported in black patients. The possibility that pigment-deficient areas may represent the target organ for sunligh-induced melanomas in Negroes and dark-skinned subjects is discussed.", "contents": "Melanomas of the lower extremity among native Puerto Ricans. The clinical features of 57 melanomas of the lower extremity occurring in native Puerto Ricans were studied. Whereas most melanomas of the lower extremity among Caucasians occur above the ankle, the group studied showed a predilection for the foot, particularly in the minimally pigmented zones (sole, heel, and nail bed), a distribution similar to that reported in black patients. The possibility that pigment-deficient areas may represent the target organ for sunligh-induced melanomas in Negroes and dark-skinned subjects is discussed.", "PMID": 953976}
{"id": "pubmed23n0032_821", "title": "Second-look operation in ovarian carcinoma: postchemotherapy.", "content": "One hundred and three patients with advanced ovarian cancer underwent a second-look operation following chemotherapy. Patients should have ten or more courses of chemotherapy before second-look operation. Those patients with no evidence of disease at second laparotomy should discontinue their chemotherapy. Patients who continued chemotherapy after the second-look operation did better than those who were treated with radiation after surgery. Only patients with clinical remission benefit from the second-look procedure. Advantages of second-look operations following chemotherapy are discussed.", "contents": "Second-look operation in ovarian carcinoma: postchemotherapy. One hundred and three patients with advanced ovarian cancer underwent a second-look operation following chemotherapy. Patients should have ten or more courses of chemotherapy before second-look operation. Those patients with no evidence of disease at second laparotomy should discontinue their chemotherapy. Patients who continued chemotherapy after the second-look operation did better than those who were treated with radiation after surgery. Only patients with clinical remission benefit from the second-look procedure. Advantages of second-look operations following chemotherapy are discussed.", "PMID": 953977}
{"id": "pubmed23n0032_822", "title": "The investigation and management of Hodgkin's disease in the pregnant patient.", "content": "The management of Hodgkin's disease during pregnancy is reviewed in the light of experience from 15 patients. The modification of the investigative procedures during pregnancy is discussed and the use of the single-film (at 24 hours) lymphogram is described. The importance of an individual treatment policy for each patient, after careful consideration of the facts of the case, is emphasized and the dangers of inadequate therapy are stressed.", "contents": "The investigation and management of Hodgkin's disease in the pregnant patient. The management of Hodgkin's disease during pregnancy is reviewed in the light of experience from 15 patients. The modification of the investigative procedures during pregnancy is discussed and the use of the single-film (at 24 hours) lymphogram is described. The importance of an individual treatment policy for each patient, after careful consideration of the facts of the case, is emphasized and the dangers of inadequate therapy are stressed.", "PMID": 953978}
{"id": "pubmed23n0032_823", "title": "Chromosomal characteristics of malignant lymphoma in northern pike (Esox lucius) from the United States.", "content": "Chromosome analyses were performed on tumor cells and normal hematopoietic cells of northern pike bearing malignant lymphomas. The normal pike karyotype contains 50 acrocentric chromosomes, among which the sex chromosomes are not identifiable by available techniques. Metaphases from 19 primary lymphomas showed a consistent pattern of chromosome anomalies consisting of 1 submetacentric marker, 1 minute marker, and 3 to 5 pairs of smaller-than-normal chromosomes, set within a mode of 50 and a range of 46 to 54. Occasional cells had 2 identical submetacentric markers. Cells from presumptively normal renal hematopoietic tissue in 41 lymphoma-bearing pike showed the euploid karyotype, with the exception of 2 cases in which some cells with the neoplastic pattern were found. This probably indicated occult neoplastic involvement of renal hematopoietic tissue, in spite of the absence of histological evidence of involvement. In many cases, some of the cells taken directly from lymphomas bore the normal karyotype. It is not known whether these cells were reactive lymphoid cells or lymphoma cells that had not developed those anomalies typically associated with the tumors. The findings do not permit a conclusion regarding agent versus cellular transmission. However, the pike lymphoma is an excellent example of a neoplasm with a karyotypic \"signature.\"", "contents": "Chromosomal characteristics of malignant lymphoma in northern pike (Esox lucius) from the United States. Chromosome analyses were performed on tumor cells and normal hematopoietic cells of northern pike bearing malignant lymphomas. The normal pike karyotype contains 50 acrocentric chromosomes, among which the sex chromosomes are not identifiable by available techniques. Metaphases from 19 primary lymphomas showed a consistent pattern of chromosome anomalies consisting of 1 submetacentric marker, 1 minute marker, and 3 to 5 pairs of smaller-than-normal chromosomes, set within a mode of 50 and a range of 46 to 54. Occasional cells had 2 identical submetacentric markers. Cells from presumptively normal renal hematopoietic tissue in 41 lymphoma-bearing pike showed the euploid karyotype, with the exception of 2 cases in which some cells with the neoplastic pattern were found. This probably indicated occult neoplastic involvement of renal hematopoietic tissue, in spite of the absence of histological evidence of involvement. In many cases, some of the cells taken directly from lymphomas bore the normal karyotype. It is not known whether these cells were reactive lymphoid cells or lymphoma cells that had not developed those anomalies typically associated with the tumors. The findings do not permit a conclusion regarding agent versus cellular transmission. However, the pike lymphoma is an excellent example of a neoplasm with a karyotypic \"signature.\"", "PMID": 953983}
{"id": "pubmed23n0032_824", "title": "Bacterial proteinaceous products (bacteriocins) as cytotoxic agents of neoplasia.", "content": "Several bacteriocins, bacterial proteinaceous antibiotics, are shown to markedly inhibit the division of various established (neoplastic) mammalian cell lines. The bacteriocins tested originated from Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, and Vibrio eltor. Using exponentially growing L60T mouse fibroblasts, the inhibitory effect was concentration dependent, and a growth inhibitory unit, equivalent to cytotoxic index 50, was established. Expression of toxicity as a function of duration of exposure to pyocin required 3 to 4 hr. DNA synthesis was inhibited and reflected the effects on growth inhibition. Maximal sensitivity to the bacteriocin was observed prior to mitosis in the G2 phase of the cell cycle.", "contents": "Bacterial proteinaceous products (bacteriocins) as cytotoxic agents of neoplasia. Several bacteriocins, bacterial proteinaceous antibiotics, are shown to markedly inhibit the division of various established (neoplastic) mammalian cell lines. The bacteriocins tested originated from Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, and Vibrio eltor. Using exponentially growing L60T mouse fibroblasts, the inhibitory effect was concentration dependent, and a growth inhibitory unit, equivalent to cytotoxic index 50, was established. Expression of toxicity as a function of duration of exposure to pyocin required 3 to 4 hr. DNA synthesis was inhibited and reflected the effects on growth inhibition. Maximal sensitivity to the bacteriocin was observed prior to mitosis in the G2 phase of the cell cycle.", "PMID": 953984}
{"id": "pubmed23n0032_825", "title": "Effect of defibrination on tumor growth and response to chemotherapy.", "content": "Other investigators have demonstrated fibrin deposition in tumors. Experiments were therefore designed to test whether systemic defibrination would alter tumor growth or tumor response to chemotherapy with cyclophosphamide. Defibrination with Ancrod, a venom extract of Agkistrodon rhodostoma, did not significantly affect tumor sensitivity to chemotherapy. Similarly, defibrination plus fibrinolytic therapy with streptokinase did not affect responsiveness to cyclophosphamide. Long-term defibrination did not affect tumor growth. These results suggest three possible interpretations: (a) the coagulation system may not be important in tumor growth and response to chemotherapy; (b) adequate clearing of fibrin from the tumor was not accomplished in our experiments; or (c) other factors such as platelet deposition may be involved and platelet function was not inhibited by the therapies used in our experiments.", "contents": "Effect of defibrination on tumor growth and response to chemotherapy. Other investigators have demonstrated fibrin deposition in tumors. Experiments were therefore designed to test whether systemic defibrination would alter tumor growth or tumor response to chemotherapy with cyclophosphamide. Defibrination with Ancrod, a venom extract of Agkistrodon rhodostoma, did not significantly affect tumor sensitivity to chemotherapy. Similarly, defibrination plus fibrinolytic therapy with streptokinase did not affect responsiveness to cyclophosphamide. Long-term defibrination did not affect tumor growth. These results suggest three possible interpretations: (a) the coagulation system may not be important in tumor growth and response to chemotherapy; (b) adequate clearing of fibrin from the tumor was not accomplished in our experiments; or (c) other factors such as platelet deposition may be involved and platelet function was not inhibited by the therapies used in our experiments.", "PMID": 953985}
{"id": "pubmed23n0032_826", "title": "Conversion of the oncogenic 1-methylguanine 3-oxide to 3-hydroxy-1-methylxanthine.", "content": "Deamination of the oncogenic 1-methylguanine 3-oxide occurs to a significant extent in rats to yield 3-hydroxy-1-methylxanthine and its metabolites. When 3-hydroxy-1-methylxanthine is administered, 1-methyl-8-methylthioxanthine can be recovered from urine and released from hepatic protein. No 1-methyl-8-methylthioguanine was detected in urine or bound to protein. There is no evidence of significant activation of 1-methylguanine 3-oxide by sulfotransferase, but deamination to the oncogenic 3-hydroxy-1-methylxanthine suffices to explain its oncogenicity.", "contents": "Conversion of the oncogenic 1-methylguanine 3-oxide to 3-hydroxy-1-methylxanthine. Deamination of the oncogenic 1-methylguanine 3-oxide occurs to a significant extent in rats to yield 3-hydroxy-1-methylxanthine and its metabolites. When 3-hydroxy-1-methylxanthine is administered, 1-methyl-8-methylthioxanthine can be recovered from urine and released from hepatic protein. No 1-methyl-8-methylthioguanine was detected in urine or bound to protein. There is no evidence of significant activation of 1-methylguanine 3-oxide by sulfotransferase, but deamination to the oncogenic 3-hydroxy-1-methylxanthine suffices to explain its oncogenicity.", "PMID": 953986}
{"id": "pubmed23n0032_827", "title": "Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A.", "content": "Macrophage-activating factor (MAF) was obtained from cultures of normal F344 rat lymphocytes incubated with insoluble concanavalin A. The MAF rendered macrophages from normal C57BL/6 mice cytotoxic against the syngeneic B16 melanoma and the allogeneic AC 15091. At the same time, normal syngeneic or allogeneic embryo cells were unharmed, even in the presence of susceptible tumor cells. Optimal MAF levels followed incubation of lymphocytes for 48 hr with Sepharose-bound concanavalin A. A 2-hr incubation of macrophages with MAF was sufficient to initiate activation, providing that 46 hr were allowed to elapse before tumor cells were added. The MAF activity was enhanced after heating the supernatant to 199 degrees. Control experiments largely excluded the possibility that residual unbound concanavalin A caused the observed macrophage-mediated tumoricidal effects.", "contents": "Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Macrophage-activating factor (MAF) was obtained from cultures of normal F344 rat lymphocytes incubated with insoluble concanavalin A. The MAF rendered macrophages from normal C57BL/6 mice cytotoxic against the syngeneic B16 melanoma and the allogeneic AC 15091. At the same time, normal syngeneic or allogeneic embryo cells were unharmed, even in the presence of susceptible tumor cells. Optimal MAF levels followed incubation of lymphocytes for 48 hr with Sepharose-bound concanavalin A. A 2-hr incubation of macrophages with MAF was sufficient to initiate activation, providing that 46 hr were allowed to elapse before tumor cells were added. The MAF activity was enhanced after heating the supernatant to 199 degrees. Control experiments largely excluded the possibility that residual unbound concanavalin A caused the observed macrophage-mediated tumoricidal effects.", "PMID": 953987}
{"id": "pubmed23n0032_828", "title": "Lack of carcinogenicity of 4-, 5-, 6-, 7-, 8-, 9-, and 10-hydroxybenzo(a)pyrene on mouse skin.", "content": "Seven phenols of benzo(a)pyrene (4-, 5-, 6-, 7-, 8-, 9-, and 10-hydroxybenzo(a)pyrene) were tested for carcinogenicity on mouse skin by application of 0.4 mumole of compound once every two weeks for 56 weeks. None of the seven phenols tested was carcinogenic to mouse skin, while treatment with the same dose of benzo(a)pyrene produced tumors in 92% of the treated animals. The lack of carcinogenicity of 7- and 8-hydroxybenzo(a)pyrene indicates that the strong carcinogenic activity previously reported for benzo(a)pyrene 7,8-oxide was not due to either phenolic isomerization product of this arene oxide.", "contents": "Lack of carcinogenicity of 4-, 5-, 6-, 7-, 8-, 9-, and 10-hydroxybenzo(a)pyrene on mouse skin. Seven phenols of benzo(a)pyrene (4-, 5-, 6-, 7-, 8-, 9-, and 10-hydroxybenzo(a)pyrene) were tested for carcinogenicity on mouse skin by application of 0.4 mumole of compound once every two weeks for 56 weeks. None of the seven phenols tested was carcinogenic to mouse skin, while treatment with the same dose of benzo(a)pyrene produced tumors in 92% of the treated animals. The lack of carcinogenicity of 7- and 8-hydroxybenzo(a)pyrene indicates that the strong carcinogenic activity previously reported for benzo(a)pyrene 7,8-oxide was not due to either phenolic isomerization product of this arene oxide.", "PMID": 953988}
{"id": "pubmed23n0032_829", "title": "Multiple sites of action of N-hydroxy-2-acetylaminofluorene rat hepatic nuclear transcription.", "content": "This study attempts to identify the site(s) of action of N-hydroxy-2-acetylaminofluorene (N-OH-AAF) in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hr after N-OH-AAF injection (3 mg/100 g body weight), rat hepatic nuclear synthesis and nucleolar RNA synthesis in vitro were inhibited by 60 and 80%, respectively. When total nuclear RNA polymerases were solubilized and assayed in the presence of alpha-amanitin (3.2 mug/ml), only alpha-amanitin-sensitive activity was reduced (50%) by N-OH-AAF. Diethylamino-ethyl-Sephadex column chromatography confirmed this finding and further demonstrated that RNA polymerase II was the activity selectively inhibited. Since N-OH-AAF dramatically inhibited nucleolar RNA synthesis but had little effect on RNA polymerase I activity, per se, we therefore concluded that, in addition to its direct inhibitory effect on the enzymic function of RNA polymerase II, N-OH-AAF must also cause impairment of the nucleolar DNA template function.", "contents": "Multiple sites of action of N-hydroxy-2-acetylaminofluorene rat hepatic nuclear transcription. This study attempts to identify the site(s) of action of N-hydroxy-2-acetylaminofluorene (N-OH-AAF) in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hr after N-OH-AAF injection (3 mg/100 g body weight), rat hepatic nuclear synthesis and nucleolar RNA synthesis in vitro were inhibited by 60 and 80%, respectively. When total nuclear RNA polymerases were solubilized and assayed in the presence of alpha-amanitin (3.2 mug/ml), only alpha-amanitin-sensitive activity was reduced (50%) by N-OH-AAF. Diethylamino-ethyl-Sephadex column chromatography confirmed this finding and further demonstrated that RNA polymerase II was the activity selectively inhibited. Since N-OH-AAF dramatically inhibited nucleolar RNA synthesis but had little effect on RNA polymerase I activity, per se, we therefore concluded that, in addition to its direct inhibitory effect on the enzymic function of RNA polymerase II, N-OH-AAF must also cause impairment of the nucleolar DNA template function.", "PMID": 953989}
{"id": "pubmed23n0032_830", "title": "Inhibition of DNA synthesis in normal and malignant human cells by triazinate (Baker's antifol) and methotrexate.", "content": "Triazinate (TZT), a triazine folate antagonist, is a potent inhibitor of dihydrofolate reductase from mammalian cells. Because antitumor activity of triazinate in experimental tumors correlated closely with the in vitro inhibition of DNA synthesis in tumor cells derived from these tumors, we studied cells from patients with leukemia, solid tumor effusions, and cells from normal marrow to determine their in vitro sensitivity to TZT. DNA synthesis in cells from patients with acute leukemia was less sensitive to TZT than it was to methotrexate (MTX) at 2 X 10(-6) M concentration of the inhibitor, whereas the sensitivity was similar at 10(-5) M. This could be accounted for by the known greater sensitivity of dihydrofolate reductase to MTX than to TZT, and the observation that, whereas intracellular drug levels were similar at low (2 X 10(-6) M) extracellular concentrations of TZT or MTX, at the higher (10(-5) M) extracellular drug concentration intracellular TZT was greater than 3 times intracellular MTX. In vitro inhibition of DNA synthesis in cells obtained after patients were treated with TZT was correlated with drug serum concentration and with leukemia cell kill. The sensitivity of cells from solid tumor effusions to TZT was similar to the sensitivity to MTX. Since patients can tolerate doses of TZT five times higher than MTX with less toxicity, there may be advantage to the clinical use of TZT in some tumor cell types.", "contents": "Inhibition of DNA synthesis in normal and malignant human cells by triazinate (Baker's antifol) and methotrexate. Triazinate (TZT), a triazine folate antagonist, is a potent inhibitor of dihydrofolate reductase from mammalian cells. Because antitumor activity of triazinate in experimental tumors correlated closely with the in vitro inhibition of DNA synthesis in tumor cells derived from these tumors, we studied cells from patients with leukemia, solid tumor effusions, and cells from normal marrow to determine their in vitro sensitivity to TZT. DNA synthesis in cells from patients with acute leukemia was less sensitive to TZT than it was to methotrexate (MTX) at 2 X 10(-6) M concentration of the inhibitor, whereas the sensitivity was similar at 10(-5) M. This could be accounted for by the known greater sensitivity of dihydrofolate reductase to MTX than to TZT, and the observation that, whereas intracellular drug levels were similar at low (2 X 10(-6) M) extracellular concentrations of TZT or MTX, at the higher (10(-5) M) extracellular drug concentration intracellular TZT was greater than 3 times intracellular MTX. In vitro inhibition of DNA synthesis in cells obtained after patients were treated with TZT was correlated with drug serum concentration and with leukemia cell kill. The sensitivity of cells from solid tumor effusions to TZT was similar to the sensitivity to MTX. Since patients can tolerate doses of TZT five times higher than MTX with less toxicity, there may be advantage to the clinical use of TZT in some tumor cell types.", "PMID": 953990}
{"id": "pubmed23n0032_831", "title": "Abnormalities of complement and its components in patients with acute leukemia, Hodgkin's disease, and sarcoma.", "content": "Whole complement and component titers were measured in patients with acute leukemia, Hodgkin's disease, and sarcoma. Serum samples were obtained from 42 consecutive patients and 11 healthy control subjects. Sera were frozen and maintained at -70 degrees until analyzed by hemolytic assay. Titers were normalized using a titer obtained from a single source of pooled human serum analyzed simultaneously with each patient sample to correct for day-to-day variation inherent in the assay technique. Significant elevations (p less than or equal to 0.05) of whole complement and C5, C8, and C9 were observed for each patient category, compared to controls. Forty-one of 42 patients had C9 titers greater than or equal to 2 S.D. above the mean titer for controls. Mean C3 and C7 titers were not elevated or depressed in any group. No clinical factors that correlated with abnormal complement or component titers were identified.", "contents": "Abnormalities of complement and its components in patients with acute leukemia, Hodgkin's disease, and sarcoma. Whole complement and component titers were measured in patients with acute leukemia, Hodgkin's disease, and sarcoma. Serum samples were obtained from 42 consecutive patients and 11 healthy control subjects. Sera were frozen and maintained at -70 degrees until analyzed by hemolytic assay. Titers were normalized using a titer obtained from a single source of pooled human serum analyzed simultaneously with each patient sample to correct for day-to-day variation inherent in the assay technique. Significant elevations (p less than or equal to 0.05) of whole complement and C5, C8, and C9 were observed for each patient category, compared to controls. Forty-one of 42 patients had C9 titers greater than or equal to 2 S.D. above the mean titer for controls. Mean C3 and C7 titers were not elevated or depressed in any group. No clinical factors that correlated with abnormal complement or component titers were identified.", "PMID": 953991}
{"id": "pubmed23n0032_832", "title": "Multiphasic incidence of foreign body-induced sarcomas.", "content": "Single or multiple plastic films (unplasticized vinyl chloride vinyl acetate copolymer) of different sizes and shapes were implanted s.c. in female CBA/H and CBA/H-T6 mice. Tumor incidence increased and accelerated with increased total surface area of multiple implants or with increased size of single implants. Tumor distribution curves over time were generally multiphasic. The profiles changed in proportionate relation to implant size. These findings indicate class differences between tumors according to latency. Since latency is known to be a predetermining characteristic of foreign body-induced tumors, class differences seem to exist at the originator cell level, reflecting diversity of intrinsic carcinogenic factors.", "contents": "Multiphasic incidence of foreign body-induced sarcomas. Single or multiple plastic films (unplasticized vinyl chloride vinyl acetate copolymer) of different sizes and shapes were implanted s.c. in female CBA/H and CBA/H-T6 mice. Tumor incidence increased and accelerated with increased total surface area of multiple implants or with increased size of single implants. Tumor distribution curves over time were generally multiphasic. The profiles changed in proportionate relation to implant size. These findings indicate class differences between tumors according to latency. Since latency is known to be a predetermining characteristic of foreign body-induced tumors, class differences seem to exist at the originator cell level, reflecting diversity of intrinsic carcinogenic factors.", "PMID": 953992}
{"id": "pubmed23n0032_833", "title": "Distribution and excretion of (3H)vincristine in the rat and the dog.", "content": "The distribution and excretion of tritiated vincristine were studies in the rat and the dog. Biphasic curves for the disappearance of the drug from blood were found in both species, with an initial half-life of approximately 15 min and a secondary half-life of approximately 75 min. Tissue levels were high in 1 hr in the rat and declined rapidly, except in the brain where very low levels of drug were found at all times. The bile was found to be the major route of excretion. The peak rate of excretion in bile was found to occur earlier in the rat (10 min) than in the dog (60 min). Rats given a higher dose of vincristine (1.0 mg/kg) excreted a larger percentage of the dose in the bile than rats given a lower dose (0.1 mg/kg). In rats given a low dose of vincristine (0.1 mg/kg), more than 85% of the drug was excreted in the feces and the urine over 72 hr. Less than 10% of the total radioactivity in the bile and urine was metabolites whereas, in the plasma, metabolites accounted for 40% of the total radioactivity.", "contents": "Distribution and excretion of (3H)vincristine in the rat and the dog. The distribution and excretion of tritiated vincristine were studies in the rat and the dog. Biphasic curves for the disappearance of the drug from blood were found in both species, with an initial half-life of approximately 15 min and a secondary half-life of approximately 75 min. Tissue levels were high in 1 hr in the rat and declined rapidly, except in the brain where very low levels of drug were found at all times. The bile was found to be the major route of excretion. The peak rate of excretion in bile was found to occur earlier in the rat (10 min) than in the dog (60 min). Rats given a higher dose of vincristine (1.0 mg/kg) excreted a larger percentage of the dose in the bile than rats given a lower dose (0.1 mg/kg). In rats given a low dose of vincristine (0.1 mg/kg), more than 85% of the drug was excreted in the feces and the urine over 72 hr. Less than 10% of the total radioactivity in the bile and urine was metabolites whereas, in the plasma, metabolites accounted for 40% of the total radioactivity.", "PMID": 953993}
{"id": "pubmed23n0032_834", "title": "The specificity of concomitant tumor immunity at large tumor volumes.", "content": "Two antigenically distinct fibrosarcomas, designated BP-8 and BP-9, induced in syngeneic C3H/HeN mice by 3,4-benzo(a)pyrene were used to study tumor-specific immunity, concomitant tumor immunity and the effect of large tumor volumes on the loss of immunological reactivity. Two groups of mice were immunized to the BP-8 tumor by amputation of growing BP-8 isografts. One group was rechallenged with the BP-8 cells, and tumor growth was not noted. Both groups of mice then received an inoculum of BP-9 cells that grew to palpable tumors to the same extent as in control mice. BP-8-immunized mice bearing progressively larger PB-9 tumors were sacrified at varying intervals after the BP-9 isograft. Tumor weight was recorded as a percentage of total body weight and viable spleen cells from these animals were tested in vitro for cytotoxicity against BP-8 and BP-9 cells in the [125I]iododeoxyuridine microcytotoxicity assay. Spleen cells from untreated mice were used as controls. The mice with growing BP-9 tumors developed an immune reaction against the tumor antigens which increased with time from initial tumor isograft and with increasing tumor size up to a definite but variable limit. Cytotoxicity to BP-9 cells rose from 18% when the BP-9 tumor was not palpable to a maximum of 77% when the tumor represented 5 to 10% of the total body weight. Cytotoxicity to BP-9 fell progressively as tumor size exceeded 15% of the total body weight and approached the 10% background cytotoxicity of control lymphocytes to BP-9 cells, when the tumor weight achieved 25% of the animal's weight. Conversely, cytotoxicity of lymphocytes against the BP-8 tumor did not vary significantly and remained about 41 to 44% over the same interval even while specific reactivity to BP-9 cells significantly decreased. In addition, with time, lymphocyte-mediated cytotoxicity to the BP-8 tumor increased from 41 to 70% if the BP-8-immunized mice had been rechallenged with antigenically identical BP-8 cells prior to the BP-9 isograft. These data suggest that loss of immunoreactivity at large tumor volumes is tumor and, presumably, antigen specific. No evidence of a generalized immune paralysis was demonstrated, since the mice always maintained immunity to the BP-8 tumor despite progressive and lethal growth of the antigenically distinct BP-9 tumor.", "contents": "The specificity of concomitant tumor immunity at large tumor volumes. Two antigenically distinct fibrosarcomas, designated BP-8 and BP-9, induced in syngeneic C3H/HeN mice by 3,4-benzo(a)pyrene were used to study tumor-specific immunity, concomitant tumor immunity and the effect of large tumor volumes on the loss of immunological reactivity. Two groups of mice were immunized to the BP-8 tumor by amputation of growing BP-8 isografts. One group was rechallenged with the BP-8 cells, and tumor growth was not noted. Both groups of mice then received an inoculum of BP-9 cells that grew to palpable tumors to the same extent as in control mice. BP-8-immunized mice bearing progressively larger PB-9 tumors were sacrified at varying intervals after the BP-9 isograft. Tumor weight was recorded as a percentage of total body weight and viable spleen cells from these animals were tested in vitro for cytotoxicity against BP-8 and BP-9 cells in the [125I]iododeoxyuridine microcytotoxicity assay. Spleen cells from untreated mice were used as controls. The mice with growing BP-9 tumors developed an immune reaction against the tumor antigens which increased with time from initial tumor isograft and with increasing tumor size up to a definite but variable limit. Cytotoxicity to BP-9 cells rose from 18% when the BP-9 tumor was not palpable to a maximum of 77% when the tumor represented 5 to 10% of the total body weight. Cytotoxicity to BP-9 fell progressively as tumor size exceeded 15% of the total body weight and approached the 10% background cytotoxicity of control lymphocytes to BP-9 cells, when the tumor weight achieved 25% of the animal's weight. Conversely, cytotoxicity of lymphocytes against the BP-8 tumor did not vary significantly and remained about 41 to 44% over the same interval even while specific reactivity to BP-9 cells significantly decreased. In addition, with time, lymphocyte-mediated cytotoxicity to the BP-8 tumor increased from 41 to 70% if the BP-8-immunized mice had been rechallenged with antigenically identical BP-8 cells prior to the BP-9 isograft. These data suggest that loss of immunoreactivity at large tumor volumes is tumor and, presumably, antigen specific. No evidence of a generalized immune paralysis was demonstrated, since the mice always maintained immunity to the BP-8 tumor despite progressive and lethal growth of the antigenically distinct BP-9 tumor.", "PMID": 953994}
{"id": "pubmed23n0032_835", "title": "Humoral regulation of cell-mediated immunity to syngeneic tumor.", "content": "Inoculation i.p. of C57BL/6 mice with FBL-3 cells, a syngeneic Friend virus-induced leukemia, results in progressive growth of ascitic tumors; in contrast, s.c. inoculation of FBL-3 cells produces transient, localized tumor growth; the recipients are then subsequently resistant to further i.p. challenge of this tumor. Experiments were performed to study the effects of humoral factors that might be present in the ascitic fluid and that could affect the growth of the tumors and the host immune response. It was found that ascitic fluids obtained from various murine tumors could indeed promote the s.c. growth of FBL-3 cells. Furthermore, administration of these ascitic fluids was found to suppress the induction of both the primary and secondary cell-mediated cytotoxic responses to FBL-3 cells in vivo and in vitro and to inhibit the effector phase of these cell-mediated cytotoxic reactions in vitro. These studies indicate that the ascitic fluids obtained from tumor-bearing hosts contain humoral factors that can promote tumor growth and suppress immune responses.", "contents": "Humoral regulation of cell-mediated immunity to syngeneic tumor. Inoculation i.p. of C57BL/6 mice with FBL-3 cells, a syngeneic Friend virus-induced leukemia, results in progressive growth of ascitic tumors; in contrast, s.c. inoculation of FBL-3 cells produces transient, localized tumor growth; the recipients are then subsequently resistant to further i.p. challenge of this tumor. Experiments were performed to study the effects of humoral factors that might be present in the ascitic fluid and that could affect the growth of the tumors and the host immune response. It was found that ascitic fluids obtained from various murine tumors could indeed promote the s.c. growth of FBL-3 cells. Furthermore, administration of these ascitic fluids was found to suppress the induction of both the primary and secondary cell-mediated cytotoxic responses to FBL-3 cells in vivo and in vitro and to inhibit the effector phase of these cell-mediated cytotoxic reactions in vitro. These studies indicate that the ascitic fluids obtained from tumor-bearing hosts contain humoral factors that can promote tumor growth and suppress immune responses.", "PMID": 953995}
{"id": "pubmed23n0032_836", "title": "Peripheral leukocyte migration inhibition reactivity to breast cancer antigens in patients with breast cancer and in normal controls.", "content": "The peripheral leukocyte migration inhibition test has been used to assess cellular immunity to soluble antigen extracts of breast cancer in patients and normal controls. In sequential tests over several weeks, 23 of 23 patients with breast cancer in remission reacted intermittently, with 67 of 139 tests (48%) being positive (greater than or equal to 20% migration inhibition). Similarly, 6 of 10 patients in relapse reacted intermittently showing 16 of 61 positive tests (26%) and 126 of 129 normal females reacted intermittently showing 135 of 512 positive tests (26%). The mean percentage of migration inhibition for all tests in patients in remission was 16.4 +/- 1.2% and that for normal controls was 7.2 +/- 0.7%; this difference was highly significant (p less than 0.001). The value for all tests in patients with relapse was 11.8 +/- 1.4%; this was statistically lower than that for patients in remission (p less than 0.05) but statistically higher than that for normal controls (p less than 0.05). A few normal women, some with high risk factors such as a strong family history and/or fibrocystic and proliferative disease, had a mean percentage of migration inhibition value in the range of that for patients with breast cancer. Mean values of sequential tests may be a more meaningful index of cellular immunity against breast cancer antigen in all groups.", "contents": "Peripheral leukocyte migration inhibition reactivity to breast cancer antigens in patients with breast cancer and in normal controls. The peripheral leukocyte migration inhibition test has been used to assess cellular immunity to soluble antigen extracts of breast cancer in patients and normal controls. In sequential tests over several weeks, 23 of 23 patients with breast cancer in remission reacted intermittently, with 67 of 139 tests (48%) being positive (greater than or equal to 20% migration inhibition). Similarly, 6 of 10 patients in relapse reacted intermittently showing 16 of 61 positive tests (26%) and 126 of 129 normal females reacted intermittently showing 135 of 512 positive tests (26%). The mean percentage of migration inhibition for all tests in patients in remission was 16.4 +/- 1.2% and that for normal controls was 7.2 +/- 0.7%; this difference was highly significant (p less than 0.001). The value for all tests in patients with relapse was 11.8 +/- 1.4%; this was statistically lower than that for patients in remission (p less than 0.05) but statistically higher than that for normal controls (p less than 0.05). A few normal women, some with high risk factors such as a strong family history and/or fibrocystic and proliferative disease, had a mean percentage of migration inhibition value in the range of that for patients with breast cancer. Mean values of sequential tests may be a more meaningful index of cellular immunity against breast cancer antigen in all groups.", "PMID": 953996}
{"id": "pubmed23n0032_837", "title": "The reversible expression of an adult isozyme locus, Gdc-1, in tumors of the mouse.", "content": "Tumors of the mouse possess 2 isozymic forms of L-glycerol-3-phosphate dehydrogenase (alpha-GPDH) (EC 1.1.1.8) that can be distinguished from each other by their heat inactivation and electrophoretic properties. These isozymes share certain structural features, since dissociation and reassociation of mixtures of the 2 isozymes lead to the generation of a hybrid molecular species. This finding suggests that the structural genes for these isozymes are closely related. A number of spontaneous and transplantable tumors of the mouse have been analyzed in order to assess whether the pattern of embryonic and adult alpha-GPDH isozyme expression is correlated with the degree of tumor differentiation. The results indicate that no correlation between the type of isozyme expressed and the degree of tumor differentiation or growth rate was evident. A striking correlation exists, however, between the physical form of the tumor and isozyme expression; all solid tumors possess, predominantly, the adult isozymic form of L-glycerol-3-phosphate dehydrogenase, whereas all ascites tumors, including embryoid bodies from ovarian and testicular teratomas, possess the embryonic form. A solid tumor, the C1300 neuroblastoma, that initially possessed the adult isozyme, was cultured in vitro; this resulted in the disappearance of the adult isozyme and predominant expression of the embryonic isozyme. Reinjection of cultured neuroblastoma cells into a host mouse produced a solid tumor that possessed the adult isozyme. The exclusive presence of either adult alpha-GPDH in solid tumor growths or embryonic alpha-GPDH in ascites tumor growths after converting from one physical forms of the tumor to the other, strongly supports a genetic regulatory mechanism which depends on the reversible repression and activation of the structural loci for these isozymes.", "contents": "The reversible expression of an adult isozyme locus, Gdc-1, in tumors of the mouse. Tumors of the mouse possess 2 isozymic forms of L-glycerol-3-phosphate dehydrogenase (alpha-GPDH) (EC 1.1.1.8) that can be distinguished from each other by their heat inactivation and electrophoretic properties. These isozymes share certain structural features, since dissociation and reassociation of mixtures of the 2 isozymes lead to the generation of a hybrid molecular species. This finding suggests that the structural genes for these isozymes are closely related. A number of spontaneous and transplantable tumors of the mouse have been analyzed in order to assess whether the pattern of embryonic and adult alpha-GPDH isozyme expression is correlated with the degree of tumor differentiation. The results indicate that no correlation between the type of isozyme expressed and the degree of tumor differentiation or growth rate was evident. A striking correlation exists, however, between the physical form of the tumor and isozyme expression; all solid tumors possess, predominantly, the adult isozymic form of L-glycerol-3-phosphate dehydrogenase, whereas all ascites tumors, including embryoid bodies from ovarian and testicular teratomas, possess the embryonic form. A solid tumor, the C1300 neuroblastoma, that initially possessed the adult isozyme, was cultured in vitro; this resulted in the disappearance of the adult isozyme and predominant expression of the embryonic isozyme. Reinjection of cultured neuroblastoma cells into a host mouse produced a solid tumor that possessed the adult isozyme. The exclusive presence of either adult alpha-GPDH in solid tumor growths or embryonic alpha-GPDH in ascites tumor growths after converting from one physical forms of the tumor to the other, strongly supports a genetic regulatory mechanism which depends on the reversible repression and activation of the structural loci for these isozymes.", "PMID": 953997}
{"id": "pubmed23n0032_838", "title": "In vivo clonogenic tumor cell kinetics following 1,3-bis(2-chloroethyl)-1-nitrosourea brain tumor therapy.", "content": "An in vitro colony formation assay was modified to determine the effects of in vivo 1,3-bis(2-chloroethyl)-1-nitrosourea therapy on tumor cell kill and subsequent clonogenic cell kinetics. The measured surviving fraction must be multiplied by the relative total number of tumor cells for each posttreatment interval in order to eliminate inaccuracies caused by dead cell removal in vivo and the lysis of damaged cells by the disaggregation procedure. The assumptions, limitations, and applications of the technique are discussed. 1,3-Bis(2-chloroethyl)-1-nitrosourea doses of 0.25, 0.50, and 1.00 X dose lethal to 10% of animals resulted in approximately at 1-, 2-, and 3-log cell kill, respectively. Significant proliferation of surviving clonogenic cells was observed after a latency period of approximately 2 days, and the rate of tumor regrowth was dose dependent. The cell-doubling times following treatment with 0.25, 0.50, and 1.00 X doses lethal to 10% of animals were 15, 21, and 38 hr, respectively. The interval to complete repopulation of the clonogenic pool corresponds to the observed increase in animal life-span for the 2 larger doses and further validates the assay as a true measure of in vivo chemotherapeutic efficacy.", "contents": "In vivo clonogenic tumor cell kinetics following 1,3-bis(2-chloroethyl)-1-nitrosourea brain tumor therapy. An in vitro colony formation assay was modified to determine the effects of in vivo 1,3-bis(2-chloroethyl)-1-nitrosourea therapy on tumor cell kill and subsequent clonogenic cell kinetics. The measured surviving fraction must be multiplied by the relative total number of tumor cells for each posttreatment interval in order to eliminate inaccuracies caused by dead cell removal in vivo and the lysis of damaged cells by the disaggregation procedure. The assumptions, limitations, and applications of the technique are discussed. 1,3-Bis(2-chloroethyl)-1-nitrosourea doses of 0.25, 0.50, and 1.00 X dose lethal to 10% of animals resulted in approximately at 1-, 2-, and 3-log cell kill, respectively. Significant proliferation of surviving clonogenic cells was observed after a latency period of approximately 2 days, and the rate of tumor regrowth was dose dependent. The cell-doubling times following treatment with 0.25, 0.50, and 1.00 X doses lethal to 10% of animals were 15, 21, and 38 hr, respectively. The interval to complete repopulation of the clonogenic pool corresponds to the observed increase in animal life-span for the 2 larger doses and further validates the assay as a true measure of in vivo chemotherapeutic efficacy.", "PMID": 953998}
{"id": "pubmed23n0032_839", "title": "Effects of grafts of single anterior pituitary glands on the incidence and type of mammary neoplasm in neutron- or gamma-irradiated Fischer female rats.", "content": "Three batches comprised of 48 young adult Fischer female rats each were subjected to total-body irradiation with 50 rads modified fission neutrons, or were given 600 rads 137Cs gamma-rays, or served as unirradiated controls. On the day following exposure, one-half of each batch was grafted with a single anterior pituitary gland beneath the left kidney capsule. The animals were observed for mammary neoplasia and all those that died during the experiment were autopsied. The experiment was terminated 538 +/- 13 days after irradiation when all neutron-irradiated, pituitary-grafted animals had one or more mammary tumors. Only 2 of the 23 untreated rats that survived until termination of the experiment developed mammary fibroadenomas, and none had mammary carcinomas. The incidence of fibroadenomas was increased, and a single carcinoma was found, in unirradiated rats with pituitary grafts. Irradiation alone caused an increase in the incidence of mammary fibroadenomas and the appearance of carcinomas. Fibroadenomas were markedly increased by the addition of pituitary grafts to irradiation. Carcinoma incidence was less markedly affected. The neutron dose of 50 rads was slightly more effective in inducing mammary neoplasms than the 600-rad dose of gamma-rays.", "contents": "Effects of grafts of single anterior pituitary glands on the incidence and type of mammary neoplasm in neutron- or gamma-irradiated Fischer female rats. Three batches comprised of 48 young adult Fischer female rats each were subjected to total-body irradiation with 50 rads modified fission neutrons, or were given 600 rads 137Cs gamma-rays, or served as unirradiated controls. On the day following exposure, one-half of each batch was grafted with a single anterior pituitary gland beneath the left kidney capsule. The animals were observed for mammary neoplasia and all those that died during the experiment were autopsied. The experiment was terminated 538 +/- 13 days after irradiation when all neutron-irradiated, pituitary-grafted animals had one or more mammary tumors. Only 2 of the 23 untreated rats that survived until termination of the experiment developed mammary fibroadenomas, and none had mammary carcinomas. The incidence of fibroadenomas was increased, and a single carcinoma was found, in unirradiated rats with pituitary grafts. Irradiation alone caused an increase in the incidence of mammary fibroadenomas and the appearance of carcinomas. Fibroadenomas were markedly increased by the addition of pituitary grafts to irradiation. Carcinoma incidence was less markedly affected. The neutron dose of 50 rads was slightly more effective in inducing mammary neoplasms than the 600-rad dose of gamma-rays.", "PMID": 953999}
{"id": "pubmed23n0032_840", "title": "Growth rates of normal and abnormal human mammary epithelia in cell culture.", "content": "The in vitro growth rates of different classifications of human mammary epithelium were compared. Samples included 4 established breast cell lines and excised tissue or breast fluid cells originating WOMEN FOR 40 DIFFERENT AND comprising 3 classifications: normal, nonmalignant atypical, and malignant. Growth was quantitated in situ and expressed as population-doubling time. Principal findings were: (a) malignant cells divided at a slower mean rate than normal cells; (b) population-doubling time values of malignant cells were more heterogenous than those of normal cells; (c) cultures from nonmalignant atypias showed population-doubling time means and standard deviations between those of normal and malignant cells; and (d) long-term mammary tumor cell lines divided more slowly than did normal cells. Discussion includes implications of data for the preneoplastic state and cell culture of mammary epithelium.", "contents": "Growth rates of normal and abnormal human mammary epithelia in cell culture. The in vitro growth rates of different classifications of human mammary epithelium were compared. Samples included 4 established breast cell lines and excised tissue or breast fluid cells originating WOMEN FOR 40 DIFFERENT AND comprising 3 classifications: normal, nonmalignant atypical, and malignant. Growth was quantitated in situ and expressed as population-doubling time. Principal findings were: (a) malignant cells divided at a slower mean rate than normal cells; (b) population-doubling time values of malignant cells were more heterogenous than those of normal cells; (c) cultures from nonmalignant atypias showed population-doubling time means and standard deviations between those of normal and malignant cells; and (d) long-term mammary tumor cell lines divided more slowly than did normal cells. Discussion includes implications of data for the preneoplastic state and cell culture of mammary epithelium.", "PMID": 954000}
{"id": "pubmed23n0032_841", "title": "Hyperplasia of the parathyroids and the pituitary in rats with experimental leydigiomas and inhibitory effect of several steroids.", "content": "Bilateral orchidectomy performed on 10- to 15-day-old Wistar rats, immediately followed by the homograft of one testis into the splenopancreatic epiploon, resulted 6 months later in leydigiomas at the site of the graft in 100% of the animals. After this latency period, the tumors continued to grow proportionately to time elapsed and were associated with both pituitary microadenomas of the aldehyde-fuchsin-, aldehyde-thionin-periodic acid-Schiff-, and periodic acid-Schiff-positive cells and diffuse hyperplasia of the parathyroids, affecting in particular the chief cells of the latter. After a 6- or 12-month leydigioma latency period, the rats were given a daily treatment by gavage for 45 days of one or two of the following steroids: Compound A, 17alpha-ethynyl, (5alpha)-androst-2-en, 17beta-ol (17 beta-acetate) (5 mg/kg when alone; 2.5 mg/kg when combined); Compound B, 17 alpha-ethynyl, (5 alpha)-androst-2-en, 17 beta-ol (17 beta-nicotinate) (always given in combined form at 2 mg/kg); Compound C, 16-oximinoestrone, 3-allyl ether (2 mg/kg when combined). The treatments caused, in various degrees, (a) involution of the leydigiomas until complete disappearance in up to 50% of the cases; (b) recovery of the characteristic morphological pattern of the pituitaries; and (c) cytolysis and interstitial fibrosis in the previously hyperplastic parathyroids.", "contents": "Hyperplasia of the parathyroids and the pituitary in rats with experimental leydigiomas and inhibitory effect of several steroids. Bilateral orchidectomy performed on 10- to 15-day-old Wistar rats, immediately followed by the homograft of one testis into the splenopancreatic epiploon, resulted 6 months later in leydigiomas at the site of the graft in 100% of the animals. After this latency period, the tumors continued to grow proportionately to time elapsed and were associated with both pituitary microadenomas of the aldehyde-fuchsin-, aldehyde-thionin-periodic acid-Schiff-, and periodic acid-Schiff-positive cells and diffuse hyperplasia of the parathyroids, affecting in particular the chief cells of the latter. After a 6- or 12-month leydigioma latency period, the rats were given a daily treatment by gavage for 45 days of one or two of the following steroids: Compound A, 17alpha-ethynyl, (5alpha)-androst-2-en, 17beta-ol (17 beta-acetate) (5 mg/kg when alone; 2.5 mg/kg when combined); Compound B, 17 alpha-ethynyl, (5 alpha)-androst-2-en, 17 beta-ol (17 beta-nicotinate) (always given in combined form at 2 mg/kg); Compound C, 16-oximinoestrone, 3-allyl ether (2 mg/kg when combined). The treatments caused, in various degrees, (a) involution of the leydigiomas until complete disappearance in up to 50% of the cases; (b) recovery of the characteristic morphological pattern of the pituitaries; and (c) cytolysis and interstitial fibrosis in the previously hyperplastic parathyroids.", "PMID": 954001}
{"id": "pubmed23n0032_842", "title": "The effect of colchicine on the induction of ornithine decarboxylase by 12-O-tetradecanoyl-phorbol-13-acetate.", "content": "The induction of mouse epidermal ornithine decarboxylase, 1 of the earliest and largest phenotypic changes following treatment of mouse skin with the tumor-promoting agent, 12-O-tetradecanoyl-phorbol-13-acetate, can be inhibited by prior administration of colchicine. Maximal inhibition of this enzyme induction was observed when colchicine was injected i.p. 90 or 120 min before promoter treatment, although time intervals up to 20 hr between colchicine and promoter treatment were effective. The effect of colchicine was dose dependent, with a dose as low as 25 nmoles/mouse causing an inhibition of 35%. Other microtubule-disrupting agents, vinblastine, vincristine, and Colcemid, had a similar effect on ornithine decarboxylase activity. However, beta, gamma-lumicolchicine, a photochemical derivative of colchicine with no antimitotic or microtubule-disrupting ability, and cytochalasin B, an inhibitor of microfilament-dependent processes, had no effect. N6, O2'-dibutyryl 3',5'-cyclic adenosine monophosphate, when administered just before colchicine, blocked the inhibitory action of colchicine. The results of these studies suggest that colchicine-sensitive structures, most likely containing microtubules, may be mediating elements between the binding of tumor promoters, perhaps to specific cell surface receptors, and the subsequent induction of ornithine decdaboxylase.", "contents": "The effect of colchicine on the induction of ornithine decarboxylase by 12-O-tetradecanoyl-phorbol-13-acetate. The induction of mouse epidermal ornithine decarboxylase, 1 of the earliest and largest phenotypic changes following treatment of mouse skin with the tumor-promoting agent, 12-O-tetradecanoyl-phorbol-13-acetate, can be inhibited by prior administration of colchicine. Maximal inhibition of this enzyme induction was observed when colchicine was injected i.p. 90 or 120 min before promoter treatment, although time intervals up to 20 hr between colchicine and promoter treatment were effective. The effect of colchicine was dose dependent, with a dose as low as 25 nmoles/mouse causing an inhibition of 35%. Other microtubule-disrupting agents, vinblastine, vincristine, and Colcemid, had a similar effect on ornithine decarboxylase activity. However, beta, gamma-lumicolchicine, a photochemical derivative of colchicine with no antimitotic or microtubule-disrupting ability, and cytochalasin B, an inhibitor of microfilament-dependent processes, had no effect. N6, O2'-dibutyryl 3',5'-cyclic adenosine monophosphate, when administered just before colchicine, blocked the inhibitory action of colchicine. The results of these studies suggest that colchicine-sensitive structures, most likely containing microtubules, may be mediating elements between the binding of tumor promoters, perhaps to specific cell surface receptors, and the subsequent induction of ornithine decdaboxylase.", "PMID": 954002}
{"id": "pubmed23n0032_843", "title": "Action of the vinca alkaloids vincristine, vinblastine, and desacetyl vinblastine amide on microtubules in vitro.", "content": "The Vinca alkaloids differ in their chemotherapeutic effectiveness and their toxicities. To determine whether differences are due to a differential effect on the assembly of tubulin into microtubules, we examined the effects of vincristine, vinblastine, and a newer alkaloid, desacetyl vinblastine amide, on the assembly of bovine brain tubulin in vitro. The three compounds block bovine tubulin polymerization in vitro and almost equally effectively at a 1 muM concentration (tubulin, 6.5 muM). At 10 muM, the three alkaloids also interact with preformed microtubules in vitro, causing spiral-like distortions of the microtubules. No effect of the alkaloids on polymerization of another fibrous protein, actin, was observed. Thus the differential actions of vinblastine, vincristine, and desacetyl vinblastine amide in vivo seems to be based on some biological process other than the reaction with tubulin or the microtubules per se.", "contents": "Action of the vinca alkaloids vincristine, vinblastine, and desacetyl vinblastine amide on microtubules in vitro. The Vinca alkaloids differ in their chemotherapeutic effectiveness and their toxicities. To determine whether differences are due to a differential effect on the assembly of tubulin into microtubules, we examined the effects of vincristine, vinblastine, and a newer alkaloid, desacetyl vinblastine amide, on the assembly of bovine brain tubulin in vitro. The three compounds block bovine tubulin polymerization in vitro and almost equally effectively at a 1 muM concentration (tubulin, 6.5 muM). At 10 muM, the three alkaloids also interact with preformed microtubules in vitro, causing spiral-like distortions of the microtubules. No effect of the alkaloids on polymerization of another fibrous protein, actin, was observed. Thus the differential actions of vinblastine, vincristine, and desacetyl vinblastine amide in vivo seems to be based on some biological process other than the reaction with tubulin or the microtubules per se.", "PMID": 954003}
{"id": "pubmed23n0032_844", "title": "Alterations in adriamycin efficacy by phenobarbital.", "content": "Adriamycin dosage should be reduced in patients with impaired liver function, since adriamycin disposition is influenced by liver metabolism and biliary excretion. It follows that drugs that increase the metabolism or excretory capacity of the liver may decrease adriamycin concentrations to suboptimal values. Adriamycin metabolism was therefore studied in mice pretreated with phenobarbital (75 mg/kg i.v.) by injection. After an i.v. dose of adriamycin (30 mg/kg i.v.), plasma fluorescence due to drug and metabolites was less and disappeared at a greater rate in phenobarbital-pretreated mice than control animals. When extracted with chloroform: isoprophyl alcohol (1:1), the livers from the phenobarbital-pretreated group yielded a greater concentration of glycones. Experiments with liver microsomes confirmed that aglycone production occurred at a more rapid initial rate in phenobarbital-induced livers. No increase in aldoketo reductase (daunorubicin reductase) activity was noted. Phenobarbital-pretreated mice, inoculated i.p. with 1 million L1210 cells and then treated with adriamycin (6 mg/kg i.v.), had significantly lower survival than did controls (p less than 0.01). These findings show that phenobarbital affects the disposition of adriamycin by microsomal enzyme induction and suggest that drugs that induce microsomal enzymes should not be used concurrently with adriamycin if optimal drug efficacy is desired.", "contents": "Alterations in adriamycin efficacy by phenobarbital. Adriamycin dosage should be reduced in patients with impaired liver function, since adriamycin disposition is influenced by liver metabolism and biliary excretion. It follows that drugs that increase the metabolism or excretory capacity of the liver may decrease adriamycin concentrations to suboptimal values. Adriamycin metabolism was therefore studied in mice pretreated with phenobarbital (75 mg/kg i.v.) by injection. After an i.v. dose of adriamycin (30 mg/kg i.v.), plasma fluorescence due to drug and metabolites was less and disappeared at a greater rate in phenobarbital-pretreated mice than control animals. When extracted with chloroform: isoprophyl alcohol (1:1), the livers from the phenobarbital-pretreated group yielded a greater concentration of glycones. Experiments with liver microsomes confirmed that aglycone production occurred at a more rapid initial rate in phenobarbital-induced livers. No increase in aldoketo reductase (daunorubicin reductase) activity was noted. Phenobarbital-pretreated mice, inoculated i.p. with 1 million L1210 cells and then treated with adriamycin (6 mg/kg i.v.), had significantly lower survival than did controls (p less than 0.01). These findings show that phenobarbital affects the disposition of adriamycin by microsomal enzyme induction and suggest that drugs that induce microsomal enzymes should not be used concurrently with adriamycin if optimal drug efficacy is desired.", "PMID": 954004}
{"id": "pubmed23n0032_845", "title": "Fetal calf serum-induced blastogenic and cytotoxic responses of human lymphocytes.", "content": "Incubation of normal human peripheral blood lymphoid cells in fetal calf serum-supplemented media initiates blastogenic and nonspecific cytotoxic responses. A panel of lymphocytes from 13 normal donors exhibited increased [3H]thymidine incorporation from 2 to 40 times that of control lymphocytes incubated in pooled human AB-supplemented media. Fetal calf serum-sensitized human lymphoid cells were active in cytotoxicity assays against a wide variety of cultured human tumor and normal target cells. Cytotoxicity continued to increase from 3 to 9 days of incubation in fetal calf serum-supplemented media, while blastogenic response peaked at 6 days of incubation. Cytotoxic and blastogenic responses increased with higher concentrations of fetal calf serum. Once stimulated with fetal calf serum, the lymphocytes did not require the continued presence of fetal calf serum to mediate a cytotoxic reaction.", "contents": "Fetal calf serum-induced blastogenic and cytotoxic responses of human lymphocytes. Incubation of normal human peripheral blood lymphoid cells in fetal calf serum-supplemented media initiates blastogenic and nonspecific cytotoxic responses. A panel of lymphocytes from 13 normal donors exhibited increased [3H]thymidine incorporation from 2 to 40 times that of control lymphocytes incubated in pooled human AB-supplemented media. Fetal calf serum-sensitized human lymphoid cells were active in cytotoxicity assays against a wide variety of cultured human tumor and normal target cells. Cytotoxicity continued to increase from 3 to 9 days of incubation in fetal calf serum-supplemented media, while blastogenic response peaked at 6 days of incubation. Cytotoxic and blastogenic responses increased with higher concentrations of fetal calf serum. Once stimulated with fetal calf serum, the lymphocytes did not require the continued presence of fetal calf serum to mediate a cytotoxic reaction.", "PMID": 954005}
{"id": "pubmed23n0032_846", "title": "A viral immunodepressive factor associated with experimental mouse tumors.", "content": "An immunodepressive factor (IDF) capable of inhibiting the rejection of allogeneic lymphomas in mice was detected in a number of transplantable mouse tumors. The allograft reactivity of the host was impaired for at least 10 months after IDF administration. No clinical evidence of bacterial or viral infection was detected in IDF-treated animals. Sera collected from mice at various intervals after injection of IDF showed a great increase in the IDF-titer. The IDF persisted in mouse sera for at least 3 months, whereas no IDF activity was found in sera of rats given injections of the factor. IDF was capable of replicating in vitro in mouse embryo cells, but not in rat embryo or HeLa cell cultures. IDF was inactivated in vitro by heat (65 degrees for 30 min), ultraviolet light or ether, but not by ethyl alcohol. These studies indicate that IDF is a virus capable of producing a long-lasting asymptomatic infection specifically interfering with the host's allograft reactivity. In several instances a close association between the lactic dehydrogenase virus and IDF was found. Nevertheless no conclusion was reached on the identity or nonidentity between the two viruses.", "contents": "A viral immunodepressive factor associated with experimental mouse tumors. An immunodepressive factor (IDF) capable of inhibiting the rejection of allogeneic lymphomas in mice was detected in a number of transplantable mouse tumors. The allograft reactivity of the host was impaired for at least 10 months after IDF administration. No clinical evidence of bacterial or viral infection was detected in IDF-treated animals. Sera collected from mice at various intervals after injection of IDF showed a great increase in the IDF-titer. The IDF persisted in mouse sera for at least 3 months, whereas no IDF activity was found in sera of rats given injections of the factor. IDF was capable of replicating in vitro in mouse embryo cells, but not in rat embryo or HeLa cell cultures. IDF was inactivated in vitro by heat (65 degrees for 30 min), ultraviolet light or ether, but not by ethyl alcohol. These studies indicate that IDF is a virus capable of producing a long-lasting asymptomatic infection specifically interfering with the host's allograft reactivity. In several instances a close association between the lactic dehydrogenase virus and IDF was found. Nevertheless no conclusion was reached on the identity or nonidentity between the two viruses.", "PMID": 954006}
{"id": "pubmed23n0032_847", "title": "Chemistry and structure-activity studies of the nitrosoureas.", "content": "The N-nitrosoureas are the most active of a variety of N-nitroso compounds tested against L1210 leukemia in mice. Structure-activity studies show that the N-(2-chloro(or fluoro)ethyl)-N-nitrosoureido grouping is necessary for high-level activity in this test system, but a variety of carrier groups can be attached to the N'-nitrogen of the urea. For high-level activity against solid tumors in rodents, eg, the Lewis lung adenocarcinoma, the N'-substituent must be a cyclohexane ring, and the most active of these compounds are substituted at position 4 of the ring. The chemistry involved in the synthesis and reactions of the nitrosoureas is described. The possible relationship of the chemically reactive species generated by the decomposition of the nitrosoureas under in vivo conditions to biologic activity is discussed.", "contents": "Chemistry and structure-activity studies of the nitrosoureas. The N-nitrosoureas are the most active of a variety of N-nitroso compounds tested against L1210 leukemia in mice. Structure-activity studies show that the N-(2-chloro(or fluoro)ethyl)-N-nitrosoureido grouping is necessary for high-level activity in this test system, but a variety of carrier groups can be attached to the N'-nitrogen of the urea. For high-level activity against solid tumors in rodents, eg, the Lewis lung adenocarcinoma, the N'-substituent must be a cyclohexane ring, and the most active of these compounds are substituted at position 4 of the ring. The chemistry involved in the synthesis and reactions of the nitrosoureas is described. The possible relationship of the chemically reactive species generated by the decomposition of the nitrosoureas under in vivo conditions to biologic activity is discussed.", "PMID": 954007}
{"id": "pubmed23n0032_848", "title": "Randomized prospective trial comparing 5-fluorouracil (NSC-19893) to 5-fluorouracil and methyl-CCNU (NSC-95441) in advanced gastrointestinal cancer.", "content": "The Southwest Oncology Group has confirmed the superiority of 5-fluorouracil (5-FU) and methyl-CCNU versus 5-FU weekly as treatment for patients with advanced gastrointestinal cancers. The drug combination produced a PR rate of 29.3% compared to the single-drug arm rate of 10.6%; however, the two-drug arm produced much more toxicity. Despite the improved response rate no improvement in survival was observed.", "contents": "Randomized prospective trial comparing 5-fluorouracil (NSC-19893) to 5-fluorouracil and methyl-CCNU (NSC-95441) in advanced gastrointestinal cancer. The Southwest Oncology Group has confirmed the superiority of 5-fluorouracil (5-FU) and methyl-CCNU versus 5-FU weekly as treatment for patients with advanced gastrointestinal cancers. The drug combination produced a PR rate of 29.3% compared to the single-drug arm rate of 10.6%; however, the two-drug arm produced much more toxicity. Despite the improved response rate no improvement in survival was observed.", "PMID": 954013}
{"id": "pubmed23n0032_849", "title": "Acute changes in high energy phosphates, nucleotide derivatives, and contractile force in ischaemic and nonischaemic canine myocardium following coronary occlusion.", "content": "Studies of ischaemic myocardium following occlusion of coronary arterial branches revealed a substantial decline in creatine phosphate, adenosine triphosphate, and total adenine nucleotide contents over 40 min. Contents of nucleotide breakdown derivatives increased significantly but transiently. Contractile force declined rapidly and stabilized at a low level. In nonischaemic muscle following occlusion creatine phosphate, adenosine triphosphate, and total nucleotide contents declined rapidly, although not to the degree seen in ischaemic muscle. Contents of derivatives increased only slightly. Contractile force increased.", "contents": "Acute changes in high energy phosphates, nucleotide derivatives, and contractile force in ischaemic and nonischaemic canine myocardium following coronary occlusion. Studies of ischaemic myocardium following occlusion of coronary arterial branches revealed a substantial decline in creatine phosphate, adenosine triphosphate, and total adenine nucleotide contents over 40 min. Contents of nucleotide breakdown derivatives increased significantly but transiently. Contractile force declined rapidly and stabilized at a low level. In nonischaemic muscle following occlusion creatine phosphate, adenosine triphosphate, and total nucleotide contents declined rapidly, although not to the degree seen in ischaemic muscle. Contents of derivatives increased only slightly. Contractile force increased.", "PMID": 954015}
{"id": "pubmed23n0032_850", "title": "An excitatory nociceptive cardiac reflex elicited by bradykinin and potentiated by prostaglandins and myocardial ischaemia.", "content": "Application of bradykinin to the exposed ventricular surface of the dog heart elicits a reflex cardiovascular response which includes a rise in blood pressure, tachycardia, renal vasoconstriction and muscular vasodilation. The reflex response depends on the dose of bradykinin and is increased by concomitant application of prostaglandin E1 or E2 and reduced by indomethacin. Temporary occlusion of the coronary artery supplying the area of the ventricle under study also sensitized the heart to topical application of bradykinin. Bradykinin and prostaglandins are released by the heart during ischaemia. We suggest, therefore, that bradykinin and prostaglandins acting in concert are the natural stimulus for excitation of the sensory receptors signalling the pain of myocardial ischaemia. We also suggest that the nervous reflex which arises from activation of sympathetic sensory nerve endings is the mechanism subserving the cardiovascular events which accompany anginal attacks.", "contents": "An excitatory nociceptive cardiac reflex elicited by bradykinin and potentiated by prostaglandins and myocardial ischaemia. Application of bradykinin to the exposed ventricular surface of the dog heart elicits a reflex cardiovascular response which includes a rise in blood pressure, tachycardia, renal vasoconstriction and muscular vasodilation. The reflex response depends on the dose of bradykinin and is increased by concomitant application of prostaglandin E1 or E2 and reduced by indomethacin. Temporary occlusion of the coronary artery supplying the area of the ventricle under study also sensitized the heart to topical application of bradykinin. Bradykinin and prostaglandins are released by the heart during ischaemia. We suggest, therefore, that bradykinin and prostaglandins acting in concert are the natural stimulus for excitation of the sensory receptors signalling the pain of myocardial ischaemia. We also suggest that the nervous reflex which arises from activation of sympathetic sensory nerve endings is the mechanism subserving the cardiovascular events which accompany anginal attacks.", "PMID": 954016}
{"id": "pubmed23n0032_851", "title": "Effect of heart rate on zonal tension and ischaemia following coronary occlusion: optimal rate for Treppe versus ischaemia.", "content": "The optimal heart rate in the immediate period following acute coronary occlusion has been controversial from the standpoint of arrhythmias and the extent of ischaemic injury. In the present study we have examined the effect of heart rate on both local myocardial contractile ability and ischaemia in 10 open chested dogs using strain gauge arches and epicardial electrograms. After sinus node destruction, atrial pacing was instituted for rate control at 100/min and increased randomly to 150, 175, and 200/min. Before coronary occlusion, total tension and rate of tension rise progressively increased at higher rates while ST segments demonstrated no significant changes. After coronary artery occlusion, total tension and rate of tension rise in the ischaemic zone decreased markedly and showed no significant change with increments in pacing rate. In the border zone, after the initial decrease in tension, pacing at 150 beats/min improved tension without a change in ST segments. However, when the rate was increased to 175 and 200 beats/min, although border zone tension increased further, ST segments rose significantly. Thus, a heart rate between 100-150/min provides the optimal range for increasing contractile ability in the nonischaemic and border zones without adversely affecting the degree of ischaemic injury.", "contents": "Effect of heart rate on zonal tension and ischaemia following coronary occlusion: optimal rate for Treppe versus ischaemia. The optimal heart rate in the immediate period following acute coronary occlusion has been controversial from the standpoint of arrhythmias and the extent of ischaemic injury. In the present study we have examined the effect of heart rate on both local myocardial contractile ability and ischaemia in 10 open chested dogs using strain gauge arches and epicardial electrograms. After sinus node destruction, atrial pacing was instituted for rate control at 100/min and increased randomly to 150, 175, and 200/min. Before coronary occlusion, total tension and rate of tension rise progressively increased at higher rates while ST segments demonstrated no significant changes. After coronary artery occlusion, total tension and rate of tension rise in the ischaemic zone decreased markedly and showed no significant change with increments in pacing rate. In the border zone, after the initial decrease in tension, pacing at 150 beats/min improved tension without a change in ST segments. However, when the rate was increased to 175 and 200 beats/min, although border zone tension increased further, ST segments rose significantly. Thus, a heart rate between 100-150/min provides the optimal range for increasing contractile ability in the nonischaemic and border zones without adversely affecting the degree of ischaemic injury.", "PMID": 954017}
{"id": "pubmed23n0032_852", "title": "Effect of hypercapnia and hypocapnia on myocardial blood flow and performance in anaesthetized dogs.", "content": "The effects of hypercapnia and hypocapnia on haemodynamics, coronary blood flow, and lactate metabolism were evaluated in anaesthetized closed chest dogs. Coronary flow increased with increased pCO2 and oxygen consumption and left ventricular performance were well maintained. Hypocapnia reduced coronary flow, oxygen consumption, and left ventricular functional performance.", "contents": "Effect of hypercapnia and hypocapnia on myocardial blood flow and performance in anaesthetized dogs. The effects of hypercapnia and hypocapnia on haemodynamics, coronary blood flow, and lactate metabolism were evaluated in anaesthetized closed chest dogs. Coronary flow increased with increased pCO2 and oxygen consumption and left ventricular performance were well maintained. Hypocapnia reduced coronary flow, oxygen consumption, and left ventricular functional performance.", "PMID": 954018}
{"id": "pubmed23n0032_853", "title": "Effect of methylprednisolone sodium succinate on hypoxic heart muscle.", "content": "The ability of 30 mg/litre methylprednisolone sodium succinate (MPSS) to modify the effects of hypoxia on isolated Langendorff-perfused rat hearts was investigated. When perfused under hypoxic conditions (pO2 less than 0.8 kPa[6 mmHg]) these hearts lose intracellular enzymes, including creatine phosphokinase (CPK) and succinic dehydrogenase (SDH). The size of the extracellular space is enhanced, the cells gain Na+ and Ca2+ and lose K+, and the endogenous stores of ATP and CP are depleted. Initially the resistance to flow in the coronary circulation falls but after 75 min of hypoxic perfusion it increases so that coronary flow is reduced. MPSS failed to prevent hypoxic muscle from either gaining Na+ and Ca2+ or losing K+. It did, however, delay the release of CPK and SDH from the hypoxic muscle, prolong the phase of increased coronary flow, and decrease the rate of depletion of the energy-rich phosphate stores. MPSS potentiated the hypoxic-induced gain in Ca2+. Whilst the effects of MPSS on coronary flow and tissue Ca2+ were probably due to the steroid part of the complex, the other changes, including the protection of the ATP and CP stores and the delayed enzyme release, were probably due to the presence of the sodium succinate.", "contents": "Effect of methylprednisolone sodium succinate on hypoxic heart muscle. The ability of 30 mg/litre methylprednisolone sodium succinate (MPSS) to modify the effects of hypoxia on isolated Langendorff-perfused rat hearts was investigated. When perfused under hypoxic conditions (pO2 less than 0.8 kPa[6 mmHg]) these hearts lose intracellular enzymes, including creatine phosphokinase (CPK) and succinic dehydrogenase (SDH). The size of the extracellular space is enhanced, the cells gain Na+ and Ca2+ and lose K+, and the endogenous stores of ATP and CP are depleted. Initially the resistance to flow in the coronary circulation falls but after 75 min of hypoxic perfusion it increases so that coronary flow is reduced. MPSS failed to prevent hypoxic muscle from either gaining Na+ and Ca2+ or losing K+. It did, however, delay the release of CPK and SDH from the hypoxic muscle, prolong the phase of increased coronary flow, and decrease the rate of depletion of the energy-rich phosphate stores. MPSS potentiated the hypoxic-induced gain in Ca2+. Whilst the effects of MPSS on coronary flow and tissue Ca2+ were probably due to the steroid part of the complex, the other changes, including the protection of the ATP and CP stores and the delayed enzyme release, were probably due to the presence of the sodium succinate.", "PMID": 954019}
{"id": "pubmed23n0032_854", "title": "Influence of training on myocardial responses of rats subjected to conditions of ischaemia and hypoxia.", "content": "Physical training has been advocated to minimize the problems associated with coronary heart disease; however, the responsible mechanisms are obscure. Rats trained to run on a treadmill were subjected to acute conditions of hypoxia and myocardial ischaemia. Trained rats were better able to maintain a higher level of cardiac performance (dP/dt max) after hypoxia than non-trained rats, but no advantages were apparent after ischaemia. Biochemical data showed no myocardial differences between the groups in oxygen utilization or energy availability.", "contents": "Influence of training on myocardial responses of rats subjected to conditions of ischaemia and hypoxia. Physical training has been advocated to minimize the problems associated with coronary heart disease; however, the responsible mechanisms are obscure. Rats trained to run on a treadmill were subjected to acute conditions of hypoxia and myocardial ischaemia. Trained rats were better able to maintain a higher level of cardiac performance (dP/dt max) after hypoxia than non-trained rats, but no advantages were apparent after ischaemia. Biochemical data showed no myocardial differences between the groups in oxygen utilization or energy availability.", "PMID": 954020}
{"id": "pubmed23n0032_855", "title": "Influence of hyperthyroidism on glycerol-extracted cardiac muscle from rabbits.", "content": "The mechanism responsible for the enhancement of myocardial contractility in hyperthyroidism is unclear. The possibility that this mechanism may involve a direct effect on the contractile proteins was investigated using the glycerol-extracted muscle strip from right ventricular papillary muscles of euthyroid rabbits and rabbits made hyperthyroid by the intraperitoneal injection of 0.25 mg/kg 1-thyroxine for 10 d. Intact papillary muscles from the hyperthyroid rabbits had an enhanced rate of tension development, a decreased time to peak tension, and a slight though insignificant increase in active tension compared to control animals. Maximal isometric contractions were induced in glycerol-extracted cardiac muscle strips from the two animal groups by the addition of 5 mmol/litre ATP and 5 mmol/litre MgCl in a buffer solution containing 0.15 mol/litre Tris-HCl (pH 7.1) at 26 degrees C. Peak isometric tension was increased in glycerinated muscle strips from hyperthyroid rabbits (1.52+/-0.10 vs 1.26 +/-0.13g/mm2), but the differences did not reach statistical significance. However, there was a marked increase in the rate of tension development in the hyperthyroid group (62.5+/-5.4 vs 41.8+/-4.7 mg/mm-2/s, P less than 0.01). This increase in the rate of isometric tension development in both intact and glycerinated muscles from hyperthyroid rabbits may be related to changes in the intrinsic turnover of actomyosin cross-bridge links in this condition. Thus, these findings suggest that thyroid hormone may influence cardiac muscle function by a direct effect on the contractile proteins.", "contents": "Influence of hyperthyroidism on glycerol-extracted cardiac muscle from rabbits. The mechanism responsible for the enhancement of myocardial contractility in hyperthyroidism is unclear. The possibility that this mechanism may involve a direct effect on the contractile proteins was investigated using the glycerol-extracted muscle strip from right ventricular papillary muscles of euthyroid rabbits and rabbits made hyperthyroid by the intraperitoneal injection of 0.25 mg/kg 1-thyroxine for 10 d. Intact papillary muscles from the hyperthyroid rabbits had an enhanced rate of tension development, a decreased time to peak tension, and a slight though insignificant increase in active tension compared to control animals. Maximal isometric contractions were induced in glycerol-extracted cardiac muscle strips from the two animal groups by the addition of 5 mmol/litre ATP and 5 mmol/litre MgCl in a buffer solution containing 0.15 mol/litre Tris-HCl (pH 7.1) at 26 degrees C. Peak isometric tension was increased in glycerinated muscle strips from hyperthyroid rabbits (1.52+/-0.10 vs 1.26 +/-0.13g/mm2), but the differences did not reach statistical significance. However, there was a marked increase in the rate of tension development in the hyperthyroid group (62.5+/-5.4 vs 41.8+/-4.7 mg/mm-2/s, P less than 0.01). This increase in the rate of isometric tension development in both intact and glycerinated muscles from hyperthyroid rabbits may be related to changes in the intrinsic turnover of actomyosin cross-bridge links in this condition. Thus, these findings suggest that thyroid hormone may influence cardiac muscle function by a direct effect on the contractile proteins.", "PMID": 954021}
{"id": "pubmed23n0032_856", "title": "Coronary occlusion before, during, and after strenuous exercise.", "content": "Groups of dogs were exercised immediately before, and immediately after occlusion of the left anterior descending coronary artery. Coronary occlusion by a staged procedure resulted in a low mortality and rare ventricular ectopic beats. The combination of simultaneous exercise with coronary occlusion reproducibly provoked ventricular fibrillation (VF). Exercise before and after occlusion frequently produced ventricular tachycardia but no VF. The risk of major arrhythmias was related to the peak heart rate resulting from the exercise.", "contents": "Coronary occlusion before, during, and after strenuous exercise. Groups of dogs were exercised immediately before, and immediately after occlusion of the left anterior descending coronary artery. Coronary occlusion by a staged procedure resulted in a low mortality and rare ventricular ectopic beats. The combination of simultaneous exercise with coronary occlusion reproducibly provoked ventricular fibrillation (VF). Exercise before and after occlusion frequently produced ventricular tachycardia but no VF. The risk of major arrhythmias was related to the peak heart rate resulting from the exercise.", "PMID": 954022}
{"id": "pubmed23n0032_857", "title": "Tissue culture, protein and collagen synthesis in antibiotic sterilized canine heart valves.", "content": "Viability of canine heart valve leaflet fibroblasts was assessed after varying periods of sterilization and storage in antibiotic-nutrient solution. Tissue culture and assessment of protein and collagen synthesis showed that tissue obtained under optimal conditions rarely retains viability beyond 3 weeks in antibiotic-nutrient solution and is severely impaired after 2 weeks. This casts serious doubts on viability in current clinical homograft valve practice.", "contents": "Tissue culture, protein and collagen synthesis in antibiotic sterilized canine heart valves. Viability of canine heart valve leaflet fibroblasts was assessed after varying periods of sterilization and storage in antibiotic-nutrient solution. Tissue culture and assessment of protein and collagen synthesis showed that tissue obtained under optimal conditions rarely retains viability beyond 3 weeks in antibiotic-nutrient solution and is severely impaired after 2 weeks. This casts serious doubts on viability in current clinical homograft valve practice.", "PMID": 954023}
{"id": "pubmed23n0032_858", "title": "Viability in human heart valves prepared for grafting.", "content": "Viability of antibiotic sterilized and stored human heart valves obtained at routine necropsy was assessed by tissue culture and protein and collagen synthesis. Only three of 23 valves examined showed any evidence of viability, in striking contrast to earlier work on canine valves obtained under optimal conditions. These findings justify doubts regarding pre-implantation viability in human heart valves prepared for grafting.", "contents": "Viability in human heart valves prepared for grafting. Viability of antibiotic sterilized and stored human heart valves obtained at routine necropsy was assessed by tissue culture and protein and collagen synthesis. Only three of 23 valves examined showed any evidence of viability, in striking contrast to earlier work on canine valves obtained under optimal conditions. These findings justify doubts regarding pre-implantation viability in human heart valves prepared for grafting.", "PMID": 954024}
{"id": "pubmed23n0032_859", "title": "Continuous imaging of regional myocardial blood flow in dogs using krypton-81m.", "content": "The unique physical properties of the freely diffusible gas krypton-81 m allowed continuous imaging of regional myocardial blood flow in dogs when infused into the aortic root. Regional changes in myocardial perfusion related to transient coronary artery occlusion were demonstrated both as high resolution gamma camera images and as a quantitative strip chart record.", "contents": "Continuous imaging of regional myocardial blood flow in dogs using krypton-81m. The unique physical properties of the freely diffusible gas krypton-81 m allowed continuous imaging of regional myocardial blood flow in dogs when infused into the aortic root. Regional changes in myocardial perfusion related to transient coronary artery occlusion were demonstrated both as high resolution gamma camera images and as a quantitative strip chart record.", "PMID": 954025}
{"id": "pubmed23n0032_860", "title": "Further studies of the epithelium covering preovulatory rabbit follicles with special reference to lysosomal alterations.", "content": "The fine structure of the surface epithelium over preovulatory rabbit follicles was examined parallel with visualization of acid phosphatase at the electron microscopical level. Small enzyme positive vesicles were pinched off from the Golgi cisternae and similar vesicles fused and got incorporated into larger lysosomes of dense body type. Some lysosomes appeared in direct continuity with tubular enzyme positive structures. Other possible ways of increase of the lysosomal pool are indicated and discussed. As in previous studies a maximal accumulation of lysosomes was found in the apical epithelium at 8 h after an ovulatory dose of human chorionic gonadotrophin (HCG); thereafter a gradual loss of lysosomes ensued. Before the lysosomes disappeared from the surface epithelium they changed in character. They became more electron-lucent and revealed a fine-fibrillar matrix. Dense bodies deep in the cell interior appeared to communicate with each other and with the extracellular space below the surface epithelium. Openings were never seen towards the peritoneal cavity. tthe loss of lysosomal content from the apical surface epithelium before follicle rupture appeared in many respects similar to the histamine release process in mast cells. tthe findings support our working hypothesis that the surface epithelium over tgraafian follicles is an essential source of proteolytic enzymes and that these may be released extracellularly and actively contribute to the dissolution of the follicular apex before rupture.", "contents": "Further studies of the epithelium covering preovulatory rabbit follicles with special reference to lysosomal alterations. The fine structure of the surface epithelium over preovulatory rabbit follicles was examined parallel with visualization of acid phosphatase at the electron microscopical level. Small enzyme positive vesicles were pinched off from the Golgi cisternae and similar vesicles fused and got incorporated into larger lysosomes of dense body type. Some lysosomes appeared in direct continuity with tubular enzyme positive structures. Other possible ways of increase of the lysosomal pool are indicated and discussed. As in previous studies a maximal accumulation of lysosomes was found in the apical epithelium at 8 h after an ovulatory dose of human chorionic gonadotrophin (HCG); thereafter a gradual loss of lysosomes ensued. Before the lysosomes disappeared from the surface epithelium they changed in character. They became more electron-lucent and revealed a fine-fibrillar matrix. Dense bodies deep in the cell interior appeared to communicate with each other and with the extracellular space below the surface epithelium. Openings were never seen towards the peritoneal cavity. tthe loss of lysosomal content from the apical surface epithelium before follicle rupture appeared in many respects similar to the histamine release process in mast cells. tthe findings support our working hypothesis that the surface epithelium over tgraafian follicles is an essential source of proteolytic enzymes and that these may be released extracellularly and actively contribute to the dissolution of the follicular apex before rupture.", "PMID": 954045}
{"id": "pubmed23n0032_861", "title": "Developmental changes in synaptic formation in the hypothalamic arcuate nucleus of female rats.", "content": "The hypothalamic arcuate nucleus (ARCN) of female rats at 5, 20, 45 and 90 days of age was examined ultrastructurally. Axodendritic and axosomatic synapses were counted in 18,000 mum2 area of the ARCN in each brain. Axodendritic and axosomatic synapses in the ARCN of day 5 rats were very small in number. Axon terminals contained small spherical vesicles (SSVs, 40-60 nm in diameter). Occasionally large granular vesicles (LGVs, 75-130 nm in diameter) were found to coexist with SSVs in the endings. Pre- and postsynaptic membranes were thin. The ARCN at this age exhibited a large extracellular space which decreased with advancing age. In day 20 rats, axodendritic and axosomatic synapses increased in number up to about one-half of those of day 45 or day 90 animals. Synaptic vesicles increased in number and mitochondria were frequently encountered in the axon terminals. Pre- and postsynaptic membranes became thicker than those of day 5 rats. Further increase in the number of axodendritic and axosomatic synapses in the ARCN of day 45 rats was observed, and there were no significant difference in the morphology and incidence of synapses between day 45 and day 90 rats. Synaptic vesicles were numerous and pre- and postsynaptic membranes were thick. In tissue incubated with 5-hydroxydopamine (5-OH-DA) before fixation, small granular vesicles (SGVs, about 50 nm in diameter) which were labeled with 5-OH-DA were detected in a certain number of endings in all material taken from each age group, but the incidence of synapses containing SGVs was usually low. From these results, it can be proposed that an increase in the number of synapses in the ARCN is correlated wihh functional maturation of the ARC neurons.", "contents": "Developmental changes in synaptic formation in the hypothalamic arcuate nucleus of female rats. The hypothalamic arcuate nucleus (ARCN) of female rats at 5, 20, 45 and 90 days of age was examined ultrastructurally. Axodendritic and axosomatic synapses were counted in 18,000 mum2 area of the ARCN in each brain. Axodendritic and axosomatic synapses in the ARCN of day 5 rats were very small in number. Axon terminals contained small spherical vesicles (SSVs, 40-60 nm in diameter). Occasionally large granular vesicles (LGVs, 75-130 nm in diameter) were found to coexist with SSVs in the endings. Pre- and postsynaptic membranes were thin. The ARCN at this age exhibited a large extracellular space which decreased with advancing age. In day 20 rats, axodendritic and axosomatic synapses increased in number up to about one-half of those of day 45 or day 90 animals. Synaptic vesicles increased in number and mitochondria were frequently encountered in the axon terminals. Pre- and postsynaptic membranes became thicker than those of day 5 rats. Further increase in the number of axodendritic and axosomatic synapses in the ARCN of day 45 rats was observed, and there were no significant difference in the morphology and incidence of synapses between day 45 and day 90 rats. Synaptic vesicles were numerous and pre- and postsynaptic membranes were thick. In tissue incubated with 5-hydroxydopamine (5-OH-DA) before fixation, small granular vesicles (SGVs, about 50 nm in diameter) which were labeled with 5-OH-DA were detected in a certain number of endings in all material taken from each age group, but the incidence of synapses containing SGVs was usually low. From these results, it can be proposed that an increase in the number of synapses in the ARCN is correlated wihh functional maturation of the ARC neurons.", "PMID": 954046}
{"id": "pubmed23n0032_862", "title": "Studies with mature rat hepatocytes in monolayer culture. I. Propagation of isolated hepatocytes, morphological and biochemical in culture.", "content": "Differentiated cells have been established in monolayer culture from adult rat liver and their ultrastructural and biochemical features characterized after 20-30 generations. Hepatocytes were isolated by enzyme perfusion of the liver followed by treatment with papain, which allowed cultures to be established more readily and to be cloned at an early stage. Ultrastructural studies indicated that the cells were derived largely from hepatic parenchymal cells. The cells showed structural modifications during primary culture but were stable thereafter. The cultured cells retained some differentiated functions unique to liver cells, including the synthesis of ornithine form arginine and the secretion of serum proteins, albumin, chi- and beta-globulins.", "contents": "Studies with mature rat hepatocytes in monolayer culture. I. Propagation of isolated hepatocytes, morphological and biochemical in culture. Differentiated cells have been established in monolayer culture from adult rat liver and their ultrastructural and biochemical features characterized after 20-30 generations. Hepatocytes were isolated by enzyme perfusion of the liver followed by treatment with papain, which allowed cultures to be established more readily and to be cloned at an early stage. Ultrastructural studies indicated that the cells were derived largely from hepatic parenchymal cells. The cells showed structural modifications during primary culture but were stable thereafter. The cultured cells retained some differentiated functions unique to liver cells, including the synthesis of ornithine form arginine and the secretion of serum proteins, albumin, chi- and beta-globulins.", "PMID": 954047}
{"id": "pubmed23n0032_863", "title": "The effects of starvation on the planarian worm Polycelis tenuis Iijima.", "content": "Employing a combination of microscopical, biochemical and autoradiographic techniques, the primary effects of starvation on adult polycelis tenuis have been studied. Over a five week period of starvation there is on average a 32% decrease in the size of the organism. This decrease is contributed to by a reduction in mitosis and an increase in cell shrinkage autolysis and death. During starvation (following a sharp rise in RNA synthesis) there is a distinct sequence of events; four peaks of acid phosphatase activity can be resolved. The first is associated with the immediate response of the gastrodermis to feeding; the second (after 6 to 7 days) with increased autophagy and dedifferentiation in the gland cells and with muscle lysis of cells. The third peak (after 14 to 15 days) is contributed to largely by the lysis of cells in the gut and the fourth peak (after 25 to 26 days) is caused by an extensive lysis of the reproductive system. Fine structural changes involving increased intracellular vacuolation, autophagy, crinophagy, atrophy of muscle, increased intercellular space and loss of basement membrane matrix have been related to changes in enzyme pattern. Nerve cells appear unchanged throughout the first five weeks of starvation. Pigment and gland cells loose their characteristic granules, dedifferentiate and become morphologically similar to the undifferentiatied neoblasts. Dedifferentiation and the mechanisms involved in the survival of starvation are discussed.", "contents": "The effects of starvation on the planarian worm Polycelis tenuis Iijima. Employing a combination of microscopical, biochemical and autoradiographic techniques, the primary effects of starvation on adult polycelis tenuis have been studied. Over a five week period of starvation there is on average a 32% decrease in the size of the organism. This decrease is contributed to by a reduction in mitosis and an increase in cell shrinkage autolysis and death. During starvation (following a sharp rise in RNA synthesis) there is a distinct sequence of events; four peaks of acid phosphatase activity can be resolved. The first is associated with the immediate response of the gastrodermis to feeding; the second (after 6 to 7 days) with increased autophagy and dedifferentiation in the gland cells and with muscle lysis of cells. The third peak (after 14 to 15 days) is contributed to largely by the lysis of cells in the gut and the fourth peak (after 25 to 26 days) is caused by an extensive lysis of the reproductive system. Fine structural changes involving increased intracellular vacuolation, autophagy, crinophagy, atrophy of muscle, increased intercellular space and loss of basement membrane matrix have been related to changes in enzyme pattern. Nerve cells appear unchanged throughout the first five weeks of starvation. Pigment and gland cells loose their characteristic granules, dedifferentiate and become morphologically similar to the undifferentiatied neoblasts. Dedifferentiation and the mechanisms involved in the survival of starvation are discussed.", "PMID": 954048}
{"id": "pubmed23n0032_864", "title": "Regional distribution of three ultrastructural retinula types in the retina of Cataglyphis bicolor Fabr. (Formicidae, Hymenoptera).", "content": "The retina of Cataglyphis bicolor was investigated by electron microscopy. Three types of structurally distinct retinulae were found and mapped throughout the compound eye: Type I is composed of four unpigmented thin cells, four larger pigmented cells as well as a basal ninth cell. Its rhabdom possesses a round cross section and four microvilli directions. This type occupies most of the dorsal two-thirds of the retina. Type II consists of two thin cells, two intermediate cells and four large cells. A basal ninth cell is also present; the rhabdom is as in type I. Type II retinulae are located in the ventral third of the retina. Type III ommatidia are unique within the Hymenoptera: there are four large pigmented cells, four thinner unpigmented cells and a basal ninth cell. The rhabdom, however, has a dumb-bell shaped cross section; two small cells lie at its opposed extremities and the remaining six cells have mutually perpendicular microvilli orientations. This type of retinula is found at the dorso-medial eye margin. Serial sectioning in this region revealed a conical shaped rhabdom without any torsion along the longitudinal axis. The rhabdomere cross section was calculated from distal and proximal thin sections. Angular statistics were applied to the microvilli directions of all three ommatidial types to determine the degree of order. A possible functional significance  of the structural specializations of the different eye regions is discussed.", "contents": "Regional distribution of three ultrastructural retinula types in the retina of Cataglyphis bicolor Fabr. (Formicidae, Hymenoptera). The retina of Cataglyphis bicolor was investigated by electron microscopy. Three types of structurally distinct retinulae were found and mapped throughout the compound eye: Type I is composed of four unpigmented thin cells, four larger pigmented cells as well as a basal ninth cell. Its rhabdom possesses a round cross section and four microvilli directions. This type occupies most of the dorsal two-thirds of the retina. Type II consists of two thin cells, two intermediate cells and four large cells. A basal ninth cell is also present; the rhabdom is as in type I. Type II retinulae are located in the ventral third of the retina. Type III ommatidia are unique within the Hymenoptera: there are four large pigmented cells, four thinner unpigmented cells and a basal ninth cell. The rhabdom, however, has a dumb-bell shaped cross section; two small cells lie at its opposed extremities and the remaining six cells have mutually perpendicular microvilli orientations. This type of retinula is found at the dorso-medial eye margin. Serial sectioning in this region revealed a conical shaped rhabdom without any torsion along the longitudinal axis. The rhabdomere cross section was calculated from distal and proximal thin sections. Angular statistics were applied to the microvilli directions of all three ommatidial types to determine the degree of order. A possible functional significance  of the structural specializations of the different eye regions is discussed.", "PMID": 954049}
{"id": "pubmed23n0032_865", "title": "The fine structure of the carapace integument of Daphnia magna Straus (Crustacea Branchiopoda).", "content": "The structure of the two integumental layers comprising the carapace of female D. magna was examined at several points through the molt cycle. The epicuticle and procuticle are simple in organisation; pore canalsare absent but intracuticular fibres are present, forming complexes with invaginations of the epidermal plasma membrane similar to such complexes described in the literature for othe arthropods. The epidermis consist almost entirely of cuticle-secreting cells. Secretion of the new cuticle begins when 50-67% of the instar has elapsed by which time the epidermal cells have increased in height and their nuclei have become more rounded. However, other presumed secretory phenomena observed viz. the formation of dense core vesicles by Golgi bodies, and the occurrence of these and coated vesicles near the apical plasma membrane are not restricted to any particular period during the molt cycle. This suggests that the mechanisms of cuticle secretion do not undergo marked changes in activity as they do in decapods; presumably this relative continunity is related to the much shorter molt cycle of cladocerans.", "contents": "The fine structure of the carapace integument of Daphnia magna Straus (Crustacea Branchiopoda). The structure of the two integumental layers comprising the carapace of female D. magna was examined at several points through the molt cycle. The epicuticle and procuticle are simple in organisation; pore canalsare absent but intracuticular fibres are present, forming complexes with invaginations of the epidermal plasma membrane similar to such complexes described in the literature for othe arthropods. The epidermis consist almost entirely of cuticle-secreting cells. Secretion of the new cuticle begins when 50-67% of the instar has elapsed by which time the epidermal cells have increased in height and their nuclei have become more rounded. However, other presumed secretory phenomena observed viz. the formation of dense core vesicles by Golgi bodies, and the occurrence of these and coated vesicles near the apical plasma membrane are not restricted to any particular period during the molt cycle. This suggests that the mechanisms of cuticle secretion do not undergo marked changes in activity as they do in decapods; presumably this relative continunity is related to the much shorter molt cycle of cladocerans.", "PMID": 954050}
{"id": "pubmed23n0032_866", "title": "Scanning electron microscopic studies on the development of the chick retina.", "content": "Fixed retinae of chick embryos and chicks of the first week after hatching were fractured and examined with the scanning electron microscope. The matrix cells of the retina proliferate up to the beginning of the second week. The migrating cells are oriented in cell cords. This columnar organizaion prevails up to the development of the plexiform layers formed as a consequence of the outgrowth of the dendritic and axonal cell processes. Special attention was paid to the differentiation of the ganglion, bipolar and receptor cells, and the radial fibers (M\u00fcller cells). Two main morphological patterns are significant for the organization of the retina during neurogenesis: a)the cell to cell contacts of migrating cells and b)the spatial arrangement of M\u00fcller cells which could provide guidelines for migration of neuronal elements.", "contents": "Scanning electron microscopic studies on the development of the chick retina. Fixed retinae of chick embryos and chicks of the first week after hatching were fractured and examined with the scanning electron microscope. The matrix cells of the retina proliferate up to the beginning of the second week. The migrating cells are oriented in cell cords. This columnar organizaion prevails up to the development of the plexiform layers formed as a consequence of the outgrowth of the dendritic and axonal cell processes. Special attention was paid to the differentiation of the ganglion, bipolar and receptor cells, and the radial fibers (M\u00fcller cells). Two main morphological patterns are significant for the organization of the retina during neurogenesis: a)the cell to cell contacts of migrating cells and b)the spatial arrangement of M\u00fcller cells which could provide guidelines for migration of neuronal elements.", "PMID": 954051}
{"id": "pubmed23n0032_867", "title": "Quantitative morphological study of smooth muscle cells of the guinea-pig taenia coli.", "content": "A quantitative study of muscle cells of the guinea-pig taenia coli is reported. Stereological methods were used on electron micrographs and phase contrast micrographs. Smooth muscle cells of taeniae fixed under 1 gram load were about 515 mum long. Muscle cell volume was about 3,500 mum3 and cell surface 5,300 mum2. About 168,000 caveolae were found at the surface of each muscle cell, covering about 29% of its surface. They produced a 73% increase of the cell membrane compared to a smooth-surfaced cell. The ratio surface-to-volume is about 1:0.67 if the geometrical surface is considered, or 1:0.39 if the total surface of the cell membrane (including the caveolae) is considered. Mitochondria constituted 3.5-4% of the cell volume. A few nexuses were observed, both between two muscle cells and between a muscle cell and an interstitial cell. In serial sections septa of connective tissue and groups of muscle cells were found to disappear within few tens of microns or to merge with other septa, and the taenia did not appear to be divided into clear-cut muscle cell bundles. Bundles of smooth muscle cells were seen passing from the taenia to the underlying circular muscle. The transverse sectional area of the taenia ranged between 0.14 and 0.39 mm2; it showed about 526 blood vessels-mm-2.", "contents": "Quantitative morphological study of smooth muscle cells of the guinea-pig taenia coli. A quantitative study of muscle cells of the guinea-pig taenia coli is reported. Stereological methods were used on electron micrographs and phase contrast micrographs. Smooth muscle cells of taeniae fixed under 1 gram load were about 515 mum long. Muscle cell volume was about 3,500 mum3 and cell surface 5,300 mum2. About 168,000 caveolae were found at the surface of each muscle cell, covering about 29% of its surface. They produced a 73% increase of the cell membrane compared to a smooth-surfaced cell. The ratio surface-to-volume is about 1:0.67 if the geometrical surface is considered, or 1:0.39 if the total surface of the cell membrane (including the caveolae) is considered. Mitochondria constituted 3.5-4% of the cell volume. A few nexuses were observed, both between two muscle cells and between a muscle cell and an interstitial cell. In serial sections septa of connective tissue and groups of muscle cells were found to disappear within few tens of microns or to merge with other septa, and the taenia did not appear to be divided into clear-cut muscle cell bundles. Bundles of smooth muscle cells were seen passing from the taenia to the underlying circular muscle. The transverse sectional area of the taenia ranged between 0.14 and 0.39 mm2; it showed about 526 blood vessels-mm-2.", "PMID": 954052}
{"id": "pubmed23n0032_868", "title": "Structural changes in smooth muscle cells during isotonic contraction.", "content": "Smooth muscle cells of the guinea-pig taenia coli were studied in light and electron microscopy, in condition of mild stretch or of isotonic contraction. During contraction the cells increase in transverse sectional area and their packing density passes from 94,000-mm-2 to 18,000-mm-2. The percentage increase in transverse sectional area of the taenia is approximately the same as the percentage decrease in length. Measurements of cell transverse sectional area suggest that the individual cells shorten and fatten more than the taenia as a whole. Whereas stretched muscle cells run parallel to each other and show a fairly smooth surface, isotonically contracted cells are twisted and entwine around each other. Their surfaces are covered with myriad processes and folds. Longitudinal, transverse or oblique stripes are seen in light microscopy in the contracted muscle cells and it is suggested that they are related to the characteristics of the cell surface. In electron microscopy a complex pattern of interdigitating finger-like and laminar processes is observed. Caveolae are mainly found on the evaginated parts of the cell surface, dense patches are mainly (but not always) found on the invaginated parts. Desmosome-like attachments between contracted cells are frequent. The collagen fibrils run approximately parallel to the stretched muscle cells; on the other hand, they run obliquely and transversely around the isotonically contracted cells.", "contents": "Structural changes in smooth muscle cells during isotonic contraction. Smooth muscle cells of the guinea-pig taenia coli were studied in light and electron microscopy, in condition of mild stretch or of isotonic contraction. During contraction the cells increase in transverse sectional area and their packing density passes from 94,000-mm-2 to 18,000-mm-2. The percentage increase in transverse sectional area of the taenia is approximately the same as the percentage decrease in length. Measurements of cell transverse sectional area suggest that the individual cells shorten and fatten more than the taenia as a whole. Whereas stretched muscle cells run parallel to each other and show a fairly smooth surface, isotonically contracted cells are twisted and entwine around each other. Their surfaces are covered with myriad processes and folds. Longitudinal, transverse or oblique stripes are seen in light microscopy in the contracted muscle cells and it is suggested that they are related to the characteristics of the cell surface. In electron microscopy a complex pattern of interdigitating finger-like and laminar processes is observed. Caveolae are mainly found on the evaginated parts of the cell surface, dense patches are mainly (but not always) found on the invaginated parts. Desmosome-like attachments between contracted cells are frequent. The collagen fibrils run approximately parallel to the stretched muscle cells; on the other hand, they run obliquely and transversely around the isotonically contracted cells.", "PMID": 954053}
{"id": "pubmed23n0032_869", "title": "Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. V. Kinetic studies on accelerated transport following caerulein infusion in vivo.", "content": "The previous finding that intracellular transport of secretory proteins in the rat exocrine pancreas is accelerated by in vivo stimulation with a pancreatic secretagogue has been further analyzed. Using a radioassay for discharge of newly synthesized proteins, the rate of release was compared in control and prestimulated lobules. In control preparations discharge occurred with an initial lag period of 30 minutes and a maximum after two hours of incubation. After in vivo infusion of 5 x 10(-8) g/hr. caerulein for 24 h in vitro discharge started after 10 minutes of in vitro incubation and attained a maximal rate after one hour. Using the same radioassay and several inhibitors of intracellular transport and granule discharge, it could be demonstrated that both processes were reduced to the same extent in controls and in lobules with accelerated transport. To obtain direct evidence for the degree of acceleration of the different transport steps between rough endoplasmic reticulum, Golgi complex and zymogen granules, the respective subcellular fractions of these organelles prepared and characterized ultrastructurally and biochemically. The rate of disappearance of newly formed proteins from rough microsomes and the appearance in smooth microsomes and zymogen granules were significantly increased after in vivo stimulation. The data substantiate an acceleration of the regular transport steps by the secretagogue. There was no indication that a high level of secretory activity leads to a rerouting of secretory proteins or to an omission of one of the regular steps in intracellular transport.", "contents": "Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. V. Kinetic studies on accelerated transport following caerulein infusion in vivo. The previous finding that intracellular transport of secretory proteins in the rat exocrine pancreas is accelerated by in vivo stimulation with a pancreatic secretagogue has been further analyzed. Using a radioassay for discharge of newly synthesized proteins, the rate of release was compared in control and prestimulated lobules. In control preparations discharge occurred with an initial lag period of 30 minutes and a maximum after two hours of incubation. After in vivo infusion of 5 x 10(-8) g/hr. caerulein for 24 h in vitro discharge started after 10 minutes of in vitro incubation and attained a maximal rate after one hour. Using the same radioassay and several inhibitors of intracellular transport and granule discharge, it could be demonstrated that both processes were reduced to the same extent in controls and in lobules with accelerated transport. To obtain direct evidence for the degree of acceleration of the different transport steps between rough endoplasmic reticulum, Golgi complex and zymogen granules, the respective subcellular fractions of these organelles prepared and characterized ultrastructurally and biochemically. The rate of disappearance of newly formed proteins from rough microsomes and the appearance in smooth microsomes and zymogen granules were significantly increased after in vivo stimulation. The data substantiate an acceleration of the regular transport steps by the secretagogue. There was no indication that a high level of secretory activity leads to a rerouting of secretory proteins or to an omission of one of the regular steps in intracellular transport.", "PMID": 954054}
{"id": "pubmed23n0032_870", "title": "Sieve structure of slit diaphragms of podocytes and pore cells of gastropod molluscs.", "content": "The ultrastructure of the slit diaphragms between the pedicels of the podocytes of the prosobranch Viviparus viviparus and between the cytoplasmic tongues of the haemocyanin producing pore cells of the pulmonate Lymnaea stagnalis was investigated. In both cell types 2 diaphragms are present in the slits. They form a 3-dimensional sieve structure with holes of respectively 90 x 110 A (podocyte) and 200 x 220 A (pore cell). Injection experiments showed that the size of the holes of the pore cell sieve matches that of particles which can be ingested by theis cell type. The substructure of the sieves of the molluscs is compared to that of the 2-dimensional sieve of the podocytes of the mouse and the rat.", "contents": "Sieve structure of slit diaphragms of podocytes and pore cells of gastropod molluscs. The ultrastructure of the slit diaphragms between the pedicels of the podocytes of the prosobranch Viviparus viviparus and between the cytoplasmic tongues of the haemocyanin producing pore cells of the pulmonate Lymnaea stagnalis was investigated. In both cell types 2 diaphragms are present in the slits. They form a 3-dimensional sieve structure with holes of respectively 90 x 110 A (podocyte) and 200 x 220 A (pore cell). Injection experiments showed that the size of the holes of the pore cell sieve matches that of particles which can be ingested by theis cell type. The substructure of the sieves of the molluscs is compared to that of the 2-dimensional sieve of the podocytes of the mouse and the rat.", "PMID": 954055}
{"id": "pubmed23n0032_871", "title": "Monoaminergic neurons in the nervous system of crustaceans.", "content": "Certain neurons in the nervous system of the malacostracan crustaceans give rise to a predominantly green and a sparse yellow fluorophore in the histochemical fluorescence method of Falck-Hillarp. The same applies to the whole of Crustacea. The green fluorophore is probably a catecholamine; the yellow to brown-yellow has not yet been identified. The biogenic amine responsible for the green fluorescence, besides being found in diffusely distributed fibres, also appears in distinct areas of fibre concentrations in the central nervous system. The protocerebrum of the malacostracans contains three areas: the central body and two areas in the top of the brain, one anterior and one posterior. The latter two are not recognized as separate areas in ordinary histological preparations. In addition, the optic neuropiles are fluorescent, some with a distinct stratification of the fluorophore. The deuto- and tritocerebrum and the ventral nerve cord also contain monoaminergic neurons. Of the brightly fluorescent areas in the whole of Crustacea, only the central body consistently exists in all species. The other areas of concentrated fluorescent neuropile are restricted to smaller taxonomic units and differ from each other.", "contents": "Monoaminergic neurons in the nervous system of crustaceans. Certain neurons in the nervous system of the malacostracan crustaceans give rise to a predominantly green and a sparse yellow fluorophore in the histochemical fluorescence method of Falck-Hillarp. The same applies to the whole of Crustacea. The green fluorophore is probably a catecholamine; the yellow to brown-yellow has not yet been identified. The biogenic amine responsible for the green fluorescence, besides being found in diffusely distributed fibres, also appears in distinct areas of fibre concentrations in the central nervous system. The protocerebrum of the malacostracans contains three areas: the central body and two areas in the top of the brain, one anterior and one posterior. The latter two are not recognized as separate areas in ordinary histological preparations. In addition, the optic neuropiles are fluorescent, some with a distinct stratification of the fluorophore. The deuto- and tritocerebrum and the ventral nerve cord also contain monoaminergic neurons. Of the brightly fluorescent areas in the whole of Crustacea, only the central body consistently exists in all species. The other areas of concentrated fluorescent neuropile are restricted to smaller taxonomic units and differ from each other.", "PMID": 954056}
{"id": "pubmed23n0032_872", "title": "Some features of the ultrastructure of the coenecium of Rhabdopleura compacta.", "content": "The coenecium of Rhabdopleura consists of a series of tubes, some erect and some repent. These tubes are composed of rings, one stacked within another. The rings are smooth on the inside surface and rough outside. Newly laid down rings are thin and smooth on both surfaces, fibrous material is laid down on the external surface during growth in thickness by the cephalic shield of the zooid. The erect tubes remain discrete, but the repent tubes, which are attached to the substratum can become incorporated in a mass of secreted material. The external vertical fibres cross several rings and probably serve to anchor the stack. Besides these fibrils that run for several segments, there are other shorter fibres that run along the length of each cylindrical ring, and are not continuous across the rings. These long and short fibres have features in common with those found in the graptolites.", "contents": "Some features of the ultrastructure of the coenecium of Rhabdopleura compacta. The coenecium of Rhabdopleura consists of a series of tubes, some erect and some repent. These tubes are composed of rings, one stacked within another. The rings are smooth on the inside surface and rough outside. Newly laid down rings are thin and smooth on both surfaces, fibrous material is laid down on the external surface during growth in thickness by the cephalic shield of the zooid. The erect tubes remain discrete, but the repent tubes, which are attached to the substratum can become incorporated in a mass of secreted material. The external vertical fibres cross several rings and probably serve to anchor the stack. Besides these fibrils that run for several segments, there are other shorter fibres that run along the length of each cylindrical ring, and are not continuous across the rings. These long and short fibres have features in common with those found in the graptolites.", "PMID": 954057}
{"id": "pubmed23n0032_873", "title": "Digestive enzyme secretion in Stomoxys calcitrans (diptera: muscidae).", "content": "Enzyme assays and morphological and histological studies show that the opaque zone midgut cells of the hematophagous fly Stomoxys calcitrans are responsible for the production of proteolytic digestive enzymes and that these are secreted into the gut lumen via membrane bound vesicles (MBV). The secretory cycle can be summarized as follows: initially the rough endoplasmic reticulum is stacked and the apices of the cells are packed with MBV. This is followed by a period of release characterized first by cytoplasmic extrusions containing high densities of MBV, then by microvesiculation of the microvilli combined with a progressive distribution of rough endoplasmic reticulum and lightening of the cellular cytoplasm. Glycogen appears in the cells at this stage and is gradually lost as the rough endoplasmic reticulum becomes stacked once more and the numbers of MBV build up again. The cycle which occurs regularly and synchronously in the cells of the zone repeats itself many times up to the completion of digestion of the blood meal. The secretory cycle is discussed with reference to activity in other secretory tissues.", "contents": "Digestive enzyme secretion in Stomoxys calcitrans (diptera: muscidae). Enzyme assays and morphological and histological studies show that the opaque zone midgut cells of the hematophagous fly Stomoxys calcitrans are responsible for the production of proteolytic digestive enzymes and that these are secreted into the gut lumen via membrane bound vesicles (MBV). The secretory cycle can be summarized as follows: initially the rough endoplasmic reticulum is stacked and the apices of the cells are packed with MBV. This is followed by a period of release characterized first by cytoplasmic extrusions containing high densities of MBV, then by microvesiculation of the microvilli combined with a progressive distribution of rough endoplasmic reticulum and lightening of the cellular cytoplasm. Glycogen appears in the cells at this stage and is gradually lost as the rough endoplasmic reticulum becomes stacked once more and the numbers of MBV build up again. The cycle which occurs regularly and synchronously in the cells of the zone repeats itself many times up to the completion of digestion of the blood meal. The secretory cycle is discussed with reference to activity in other secretory tissues.", "PMID": 954058}
{"id": "pubmed23n0032_874", "title": "Mast cell activation and tissue cell proliferation.", "content": "The effect of mast cell activation and degranulation on the proliferation in the intact mesentery was studied in Sprague-Dawley rats. Mast cell activation was achieved by a single intraperitoneal injection of Compound 48/80. The proliferation was studied using three independent methods for estimation of cell production and DNA synthesis: 1. the mitotic index, 2. the relative number of cells having a DNA content in the S and G2 regions, by Feulgen photometric measurement in individual cells, and 3. the specific DNA activity, employing a method which combines a liquid scintillation technique after an intravenous injection of 3H-thymidine and Feulgen photometric determination of the DNA content per membrane preparation. It was found that the proliferation of the normal mesenchymal cells adjacent to the activated and degranulated mast cells in the mesentery was significantly increased within 24 and 32 h, the maximum increase being more than 20-fold compared to untreated controls. The results suggest that the common type of mast cell may have a pathophysiological function related to stimulation of local cell proliferation.", "contents": "Mast cell activation and tissue cell proliferation. The effect of mast cell activation and degranulation on the proliferation in the intact mesentery was studied in Sprague-Dawley rats. Mast cell activation was achieved by a single intraperitoneal injection of Compound 48/80. The proliferation was studied using three independent methods for estimation of cell production and DNA synthesis: 1. the mitotic index, 2. the relative number of cells having a DNA content in the S and G2 regions, by Feulgen photometric measurement in individual cells, and 3. the specific DNA activity, employing a method which combines a liquid scintillation technique after an intravenous injection of 3H-thymidine and Feulgen photometric determination of the DNA content per membrane preparation. It was found that the proliferation of the normal mesenchymal cells adjacent to the activated and degranulated mast cells in the mesentery was significantly increased within 24 and 32 h, the maximum increase being more than 20-fold compared to untreated controls. The results suggest that the common type of mast cell may have a pathophysiological function related to stimulation of local cell proliferation.", "PMID": 954059}
{"id": "pubmed23n0032_875", "title": "Ovarian follicles of normal NMRI mice and homozygous \"nude\" mice. 1. Quantitative methodological study in the pubescent \"nude\" mouse.", "content": "It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous \"Nude\" mice. Under experimental conditions, the follicles were not spherical but could be assimilated to prolate ellipsoids with a mean eccentricity of 0.81. The average radius of the follicles taken as a whole was 20.2+/-0.7 (S.E.) mum. From these, the average radius of only the non-primary follicles was 58+/-1.3 (S.E.) mum. The volumetric repartition of the ovarian follicles indicated that 62% of the total follicular mass was made of follicles with a radius greater than 115 mum, although these represent only 2% of the total number of follicles.", "contents": "Ovarian follicles of normal NMRI mice and homozygous \"nude\" mice. 1. Quantitative methodological study in the pubescent \"nude\" mouse. It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous \"Nude\" mice. Under experimental conditions, the follicles were not spherical but could be assimilated to prolate ellipsoids with a mean eccentricity of 0.81. The average radius of the follicles taken as a whole was 20.2+/-0.7 (S.E.) mum. From these, the average radius of only the non-primary follicles was 58+/-1.3 (S.E.) mum. The volumetric repartition of the ovarian follicles indicated that 62% of the total follicular mass was made of follicles with a radius greater than 115 mum, although these represent only 2% of the total number of follicles.", "PMID": 954060}
{"id": "pubmed23n0032_876", "title": "Ultrastructure of the membrane attachment sites of the extrusomes of Ciliophrys marina and Heterophrys marina (Actinopoda).", "content": "The actinopods Ciliophrys marina and Heterophrys marina both have membrane bounded extrusomes attached to their cellular and axopodial membranes. The extrusomes of C. marina, the muciferous bodies, are fairly simple in structure and contain a homogeneous osmiophilic substance. Their attachment site is characterized by a rectangular array of freeze fracture particles in the cell membrane. The extrusomes of H. marina, the conicysts, are more complex and contain a two-part osmiophilic body. The attachment site of conicysts is characterized by a rosette of 8 freeze fracture particles very similar to the 9-particle rosette found at the mucocyst attachment sites in Tetrahymena. Furthermore, intracytoplasmic bridges connect the conicyst and cell membrane faces, and a specialized fibrillar structure is found on the cell membrane in the region of conicyst attachment. The various possible roles for such particle arrays are discussed and their presence in virtually all extrusomes is predicted.", "contents": "Ultrastructure of the membrane attachment sites of the extrusomes of Ciliophrys marina and Heterophrys marina (Actinopoda). The actinopods Ciliophrys marina and Heterophrys marina both have membrane bounded extrusomes attached to their cellular and axopodial membranes. The extrusomes of C. marina, the muciferous bodies, are fairly simple in structure and contain a homogeneous osmiophilic substance. Their attachment site is characterized by a rectangular array of freeze fracture particles in the cell membrane. The extrusomes of H. marina, the conicysts, are more complex and contain a two-part osmiophilic body. The attachment site of conicysts is characterized by a rosette of 8 freeze fracture particles very similar to the 9-particle rosette found at the mucocyst attachment sites in Tetrahymena. Furthermore, intracytoplasmic bridges connect the conicyst and cell membrane faces, and a specialized fibrillar structure is found on the cell membrane in the region of conicyst attachment. The various possible roles for such particle arrays are discussed and their presence in virtually all extrusomes is predicted.", "PMID": 954061}
{"id": "pubmed23n0032_877", "title": "Enveloping layer and periderm of the trout embryo (Salmo trutta fario L.)20U.", "content": "The origin of pericarderm and epidermis has been studied in trout embryos from stage 20(2 days after fertilization at 10degrees c) to hatching (stage 410). Between stages 20 and 50, the blastodisc consists of an inner mass of blastomeres covered by a superficial layer of closely packed blastomeres, the peripheral layer. This layer gives rise to both peripheral cells and to cells joining the inner mass of blastomeres. Between stages 50 and 110, junctions differentiate between peripheral cells. This newly formed superficial epithelium the enveloping layer, no longer gives rise to inward migrating cells. From stage 110 on, a basement membrane differentiates beneath a one-cell thick subperipheral layer, which thus becomes the ectodermal basal layer, the prospective epidermal basal layer. From these and ultrastructural observations, it is concluded that 1)the epidermis apparently originates, at least in part from the peripheral cells, between stages 20 and 50; 2) the periderm assumes a protective function over the body of the embryo and also a secretory function over the yolk sac (probably producing the hatching enzymes); 3)the periderm, which is a temporary structure, appears to play the role of an embryonic membrane in teleosts.", "contents": "Enveloping layer and periderm of the trout embryo (Salmo trutta fario L.)20U. The origin of pericarderm and epidermis has been studied in trout embryos from stage 20(2 days after fertilization at 10degrees c) to hatching (stage 410). Between stages 20 and 50, the blastodisc consists of an inner mass of blastomeres covered by a superficial layer of closely packed blastomeres, the peripheral layer. This layer gives rise to both peripheral cells and to cells joining the inner mass of blastomeres. Between stages 50 and 110, junctions differentiate between peripheral cells. This newly formed superficial epithelium the enveloping layer, no longer gives rise to inward migrating cells. From stage 110 on, a basement membrane differentiates beneath a one-cell thick subperipheral layer, which thus becomes the ectodermal basal layer, the prospective epidermal basal layer. From these and ultrastructural observations, it is concluded that 1)the epidermis apparently originates, at least in part from the peripheral cells, between stages 20 and 50; 2) the periderm assumes a protective function over the body of the embryo and also a secretory function over the yolk sac (probably producing the hatching enzymes); 3)the periderm, which is a temporary structure, appears to play the role of an embryonic membrane in teleosts.", "PMID": 954062}
{"id": "pubmed23n0032_878", "title": "The Wolff-Parkinson-White syndrome: the value of the HIS bundle electrogram,.", "content": "The HIS bundle electrogram has led to the aquisition of additional information on the physiology of the WPW syndrome and has become a useful technique for its diagnosis. The findings on the HBE and their interpretation in WPW are reviewed.", "contents": "The Wolff-Parkinson-White syndrome: the value of the HIS bundle electrogram,. The HIS bundle electrogram has led to the aquisition of additional information on the physiology of the WPW syndrome and has become a useful technique for its diagnosis. The findings on the HBE and their interpretation in WPW are reviewed.", "PMID": 954068}
{"id": "pubmed23n0032_879", "title": "Right ventricular mass estimation by angioechocardiography.", "content": "A combined angiocardiographic-echocardiographic method for estimating right ventricular wall mass is described. Biplane cineangiocardiograms are analyzed for ventricular volume in end-diastole, and wall thickness is determined from echocardiograms obtained with a high frequency transducer and strip chart recorder, The intracavitary and the external surface volumes of the ventricle are derived, and the difference multiplied by 1.050, the specific gravity of myocardium. Excellent correlation was observed between right ventricular wall mass and body surface area in normal children (r = 0.93). The mean right ventricular mass was 44.5 g/M2 as compared to 78.1 g/M2 for the left ventricle, corresponding mass/EDV values were 0.48 g/cm3 and 1.26 g/cm3, respectively. In isolated right ventricular pressure overload, the increase in right ventricular mass is chiefly due to the increase in wall thickness; in volume overload, it is due mostly to the increase in chamber volume,", "contents": "Right ventricular mass estimation by angioechocardiography. A combined angiocardiographic-echocardiographic method for estimating right ventricular wall mass is described. Biplane cineangiocardiograms are analyzed for ventricular volume in end-diastole, and wall thickness is determined from echocardiograms obtained with a high frequency transducer and strip chart recorder, The intracavitary and the external surface volumes of the ventricle are derived, and the difference multiplied by 1.050, the specific gravity of myocardium. Excellent correlation was observed between right ventricular wall mass and body surface area in normal children (r = 0.93). The mean right ventricular mass was 44.5 g/M2 as compared to 78.1 g/M2 for the left ventricle, corresponding mass/EDV values were 0.48 g/cm3 and 1.26 g/cm3, respectively. In isolated right ventricular pressure overload, the increase in right ventricular mass is chiefly due to the increase in wall thickness; in volume overload, it is due mostly to the increase in chamber volume,", "PMID": 954069}
{"id": "pubmed23n0032_880", "title": "Cine angiographic diagnosis of patent foramen ovale in paradoxical embolism,.", "content": "A case of paradoxical embolism is presented in which the antemortem diagnosis was facilitated by a new simple angiographic technique that demonstrated a patent foramen ovale. An additional 14 patients were studied by this technique, and one had a patent foramen ovale. The mechanism of reversal of the normal intraatrial pressure gradient and subsequent right to left shunt through a patent foramen ovale is discussed. This angiographic technique may be readily applied in any diagnostic catheterization laboratory and complements existing methods for detecting patency of the foramen ovale in patients with suspected paradoxidal embolism.", "contents": "Cine angiographic diagnosis of patent foramen ovale in paradoxical embolism,. A case of paradoxical embolism is presented in which the antemortem diagnosis was facilitated by a new simple angiographic technique that demonstrated a patent foramen ovale. An additional 14 patients were studied by this technique, and one had a patent foramen ovale. The mechanism of reversal of the normal intraatrial pressure gradient and subsequent right to left shunt through a patent foramen ovale is discussed. This angiographic technique may be readily applied in any diagnostic catheterization laboratory and complements existing methods for detecting patency of the foramen ovale in patients with suspected paradoxidal embolism.", "PMID": 954070}
{"id": "pubmed23n0032_881", "title": "The evaluation of routine selective arteriography in the investigation of renovascular hypertension.", "content": "A comparison of lesions discovered by selective renal arteriograms with abnormalities observed on abdominal aortograms was made in 100 consecutive hypertensive patients. Twenty-one lesions not seen on aortograms were found by selection. Eight patients with a normal aortogram had lesions. An abnormality was discovered in at least one study in 58 patients. The increased rate of lesion detection by selective studies outweighs the slight risk of complications.", "contents": "The evaluation of routine selective arteriography in the investigation of renovascular hypertension. A comparison of lesions discovered by selective renal arteriograms with abnormalities observed on abdominal aortograms was made in 100 consecutive hypertensive patients. Twenty-one lesions not seen on aortograms were found by selection. Eight patients with a normal aortogram had lesions. An abnormality was discovered in at least one study in 58 patients. The increased rate of lesion detection by selective studies outweighs the slight risk of complications.", "PMID": 954071}
{"id": "pubmed23n0032_882", "title": "Aortic stenosis, angina pectoris, coronary artery disease.", "content": "The data from 88 patients (pts) with aortic stenosis (AS) were reviewed to determine relationships between angina pectoris (AP) and coronary artery disease (CAD). Results of surgery performed in 81 of these pts was analyzed. All pts had coronary arteriograms, and lesions greater than or equal to 50% were considered significant. Fifty-nine pts had an aortic valve gradient measured at catheterization greater than or equal to 40 mmHg, and in 29 pts, AS was confirmed at operation. Sixty-eight pts (77%) experienced AP, and 32 had coexisting CAD (47%); 9 of 20 pts without AP had CAD (45%). There were no significant differences in the incidence of AP in pts divided into subgroups by the aortic valve gradient (40-50, 51-100, 101-200 mmHg) or age (40-59, 60-81 years). Also, no significant differences were found in the incidence or extent of CAD between the two age groups; the extent of CAD was similar regardless of the presence or absence of AP. In pts with AP (1) CAD was more likely in pts greater than or equal to 60 years of age; (2) CAD was less likely when the aortic valve gradient was greater than 100 mmHg, suggesting that AP in these pts was due to hemodynamically severe AS. All pts with 3-vessel CAD experienced AP, and the aortic valve gradient was less in these pts than in those with no CAD or less extensive CAD. In 19 pts with combined AS and CAD who had both the aortic valve replaced and a revascularization operation only 1 of pts died in the hospital, while 3 of 19 pts with combined AS and CAD who had aortic valve replacement alone died. In this study a significant number of pts with AS experienced AP, and the presence or absence of AP did not predict coexisting CAD. Coronary arteriography is recommended in the evaluation of pts greater than or equal to 40 years of age with AS. The operative mortality appears to be decreased in pts with AS and CAD who have combined surgery.", "contents": "Aortic stenosis, angina pectoris, coronary artery disease. The data from 88 patients (pts) with aortic stenosis (AS) were reviewed to determine relationships between angina pectoris (AP) and coronary artery disease (CAD). Results of surgery performed in 81 of these pts was analyzed. All pts had coronary arteriograms, and lesions greater than or equal to 50% were considered significant. Fifty-nine pts had an aortic valve gradient measured at catheterization greater than or equal to 40 mmHg, and in 29 pts, AS was confirmed at operation. Sixty-eight pts (77%) experienced AP, and 32 had coexisting CAD (47%); 9 of 20 pts without AP had CAD (45%). There were no significant differences in the incidence of AP in pts divided into subgroups by the aortic valve gradient (40-50, 51-100, 101-200 mmHg) or age (40-59, 60-81 years). Also, no significant differences were found in the incidence or extent of CAD between the two age groups; the extent of CAD was similar regardless of the presence or absence of AP. In pts with AP (1) CAD was more likely in pts greater than or equal to 60 years of age; (2) CAD was less likely when the aortic valve gradient was greater than 100 mmHg, suggesting that AP in these pts was due to hemodynamically severe AS. All pts with 3-vessel CAD experienced AP, and the aortic valve gradient was less in these pts than in those with no CAD or less extensive CAD. In 19 pts with combined AS and CAD who had both the aortic valve replaced and a revascularization operation only 1 of pts died in the hospital, while 3 of 19 pts with combined AS and CAD who had aortic valve replacement alone died. In this study a significant number of pts with AS experienced AP, and the presence or absence of AP did not predict coexisting CAD. Coronary arteriography is recommended in the evaluation of pts greater than or equal to 40 years of age with AS. The operative mortality appears to be decreased in pts with AS and CAD who have combined surgery.", "PMID": 954072}
{"id": "pubmed23n0032_883", "title": "Ejection time--heart rate relationship during exercise.", "content": "The slope of the regression equation for left ventricular ejection time (LVET) vs heart rate (HR) is the appropriate factor for correcting LVET for HR. Because the regression relationship varies under different conditions, we determined the LVET-HR equations for subjects (1) seated at rest on a bicycle ergometer, and (2) during uninterrupted bicycle exercise. In 18 normally active male volunteers, ages 22-37, HR and LVET were measured under the two conditions and the regression relationship for LVET on HR determined for each. Regression equations are as follows: (1) LVET = 379-1.8 HR +/- 11.0 for subjects seated at rest on a bicycle ergometer, and (2) LVET = 371-1.2 HR +/- 13.9 for subjects performing upright bicycle exercise. The slope factors (1.8 and 1.2) differed significantly (p less than 0.01). The data indicate that considerable error can result from arbitrarily applying to exercising or resting subjects a correction factor which does not fit the conditions of the data to be corrected.", "contents": "Ejection time--heart rate relationship during exercise. The slope of the regression equation for left ventricular ejection time (LVET) vs heart rate (HR) is the appropriate factor for correcting LVET for HR. Because the regression relationship varies under different conditions, we determined the LVET-HR equations for subjects (1) seated at rest on a bicycle ergometer, and (2) during uninterrupted bicycle exercise. In 18 normally active male volunteers, ages 22-37, HR and LVET were measured under the two conditions and the regression relationship for LVET on HR determined for each. Regression equations are as follows: (1) LVET = 379-1.8 HR +/- 11.0 for subjects seated at rest on a bicycle ergometer, and (2) LVET = 371-1.2 HR +/- 13.9 for subjects performing upright bicycle exercise. The slope factors (1.8 and 1.2) differed significantly (p less than 0.01). The data indicate that considerable error can result from arbitrarily applying to exercising or resting subjects a correction factor which does not fit the conditions of the data to be corrected.", "PMID": 954073}
{"id": "pubmed23n0032_884", "title": "Potential artifact in measurement of left ventricular filling pressure with flow-directed catheters.", "content": "Artifactual pulmonary wedge pressure measurements were encountered during bedside use of Swan-Ganz catheters. These values were higher than pulmonary artery end diastolic pressure and devoid of typical phasic contours. Utilizing fluoroscopy these artifacts were reproduced by advancing the catheter tip 2-4 cm beyond the site of initial wedging and reinflating the balloon. Spurious increases of pulmonary wedge pressure of up to 15 mm Hg were thus obtained. In vitro catheter testing demonstrated overlapping of the catheter tip by the deformed balloon when it was fully inflated in a channel too small to accomodate it. These measurement artifacts are thus attributed to distal migration of the catheter tip into relatively small pulmonary artery branches and to subsequent occlusion of the catheter lumen by the balloon when it is reinflated. This can readily be avoided by routinely inflating the balloon with the minimum volume of air sufficient to yield a pulmonary wedge pressure tracing.", "contents": "Potential artifact in measurement of left ventricular filling pressure with flow-directed catheters. Artifactual pulmonary wedge pressure measurements were encountered during bedside use of Swan-Ganz catheters. These values were higher than pulmonary artery end diastolic pressure and devoid of typical phasic contours. Utilizing fluoroscopy these artifacts were reproduced by advancing the catheter tip 2-4 cm beyond the site of initial wedging and reinflating the balloon. Spurious increases of pulmonary wedge pressure of up to 15 mm Hg were thus obtained. In vitro catheter testing demonstrated overlapping of the catheter tip by the deformed balloon when it was fully inflated in a channel too small to accomodate it. These measurement artifacts are thus attributed to distal migration of the catheter tip into relatively small pulmonary artery branches and to subsequent occlusion of the catheter lumen by the balloon when it is reinflated. This can readily be avoided by routinely inflating the balloon with the minimum volume of air sufficient to yield a pulmonary wedge pressure tracing.", "PMID": 954074}
{"id": "pubmed23n0032_885", "title": "A new wedge to facilitate obtaining half-axial views during coronary arteriography.", "content": "A wedge has been designed and constructed to facilitate obtaining posteroanterior and oblique half-axial projections routinely during coronary arteriographic examinations in a conventionally equipped catheterization laboratory.", "contents": "A new wedge to facilitate obtaining half-axial views during coronary arteriography. A wedge has been designed and constructed to facilitate obtaining posteroanterior and oblique half-axial projections routinely during coronary arteriographic examinations in a conventionally equipped catheterization laboratory.", "PMID": 954075}
{"id": "pubmed23n0032_886", "title": "A semiautomated technique for the rapid evaluation of left ventricular regional wall motion.", "content": "A semiautomated system for the rapid evaluation of left ventricular regional wall motion is described. The system includes direct projection of the cineangiogram film on an X-Y digitizer which is interfaced with a PDP-9 computer. The ventricular model used to evaluate regional wall motion and the methods of data analysis are described. Validation of the accuracy of the technique and specific clinical applications are presented. It is concluded that this technique provides a rapid means of evaluating left ventricular regional wall motion and that the accuracy of the technique is acceptable for clinical application.", "contents": "A semiautomated technique for the rapid evaluation of left ventricular regional wall motion. A semiautomated system for the rapid evaluation of left ventricular regional wall motion is described. The system includes direct projection of the cineangiogram film on an X-Y digitizer which is interfaced with a PDP-9 computer. The ventricular model used to evaluate regional wall motion and the methods of data analysis are described. Validation of the accuracy of the technique and specific clinical applications are presented. It is concluded that this technique provides a rapid means of evaluating left ventricular regional wall motion and that the accuracy of the technique is acceptable for clinical application.", "PMID": 954076}
{"id": "pubmed23n0032_887", "title": "A simple method for calculating left ventricular functions from angiographic data using a programmable hand calculator.", "content": "The end diastolic volume and systolic ejection fraction have gained increasing acceptance as important indicators of ventricular performance. Time consuming calculations and lack of computer facilities have hindered the emergence of these calculations as a routine part of cardiac catheterization studies. The introduction of the programmable hand calculator has provided means for rapid analysis of ventricular volume data in an efficient and inexpensive manner. In this paper the step-by-step procedure for programming the hand calculator is given, as well as instructions for entering raw data and obtaining final calculations. Programs are given for both single plane and biplane cine angiographic studies.", "contents": "A simple method for calculating left ventricular functions from angiographic data using a programmable hand calculator. The end diastolic volume and systolic ejection fraction have gained increasing acceptance as important indicators of ventricular performance. Time consuming calculations and lack of computer facilities have hindered the emergence of these calculations as a routine part of cardiac catheterization studies. The introduction of the programmable hand calculator has provided means for rapid analysis of ventricular volume data in an efficient and inexpensive manner. In this paper the step-by-step procedure for programming the hand calculator is given, as well as instructions for entering raw data and obtaining final calculations. Programs are given for both single plane and biplane cine angiographic studies.", "PMID": 954077}
{"id": "pubmed23n0032_888", "title": "Correlation between structural transformation and cleavage of the major head protein of T4 bacteriophage.", "content": "We have studied the maturation of T4 polyheads, the aberrant tubular structures related to the capsid of T4 bacteriophage. Conditions have been found under which more than 95% of the major head protein (P23) undergoes the same cleavage that occurs during development of the normal capsid. The concomitant structural changes in the polyheads have been followed using electron microscope image filtering techniques. As a result of the cleavage, a radical transformation of the hexagonal lattice occurs, involving a 10-15% expansion in the lattice dimensions. However, a metastable intermediate state similar to the uncleaved structure has been observed immediately after cleavage of the protein subunits. Some kind of additional physical stimulus seems to be required to trigger the major structural change, which appears to be highly cooperative.", "contents": "Correlation between structural transformation and cleavage of the major head protein of T4 bacteriophage. We have studied the maturation of T4 polyheads, the aberrant tubular structures related to the capsid of T4 bacteriophage. Conditions have been found under which more than 95% of the major head protein (P23) undergoes the same cleavage that occurs during development of the normal capsid. The concomitant structural changes in the polyheads have been followed using electron microscope image filtering techniques. As a result of the cleavage, a radical transformation of the hexagonal lattice occurs, involving a 10-15% expansion in the lattice dimensions. However, a metastable intermediate state similar to the uncleaved structure has been observed immediately after cleavage of the protein subunits. Some kind of additional physical stimulus seems to be required to trigger the major structural change, which appears to be highly cooperative.", "PMID": 954078}
{"id": "pubmed23n0032_889", "title": "Lack of correlation between agglutinability, the surface distribution of con A and post-confluence inhibition of cell division in ten cell lines.", "content": "Agglutinability by concanavalin A, distribution of surface-bound concanavalin A, and maximal cell density in monolayer culture were examined under similar conditions in parallel cultures of ten established cell lines. The degree of agglutinability of the cell lines did not correlate with the presence or absence of patching of concanavalin A bound to the cell surface, as determined with a hemocyanin marker. Agglutinability was also not always correlated with the loss of post-confluence inhibition of cell division. Two clones of mouse 3T3 fibroblasts that maintained post-confluence inhibition of cell division and low agglutinability differed substantially with respect to the surface distribution of concanavalin A. Patching of concanavalin A binding sites is neither necessary nor sufficient to explain differences in agglutinability between cell lines.", "contents": "Lack of correlation between agglutinability, the surface distribution of con A and post-confluence inhibition of cell division in ten cell lines. Agglutinability by concanavalin A, distribution of surface-bound concanavalin A, and maximal cell density in monolayer culture were examined under similar conditions in parallel cultures of ten established cell lines. The degree of agglutinability of the cell lines did not correlate with the presence or absence of patching of concanavalin A bound to the cell surface, as determined with a hemocyanin marker. Agglutinability was also not always correlated with the loss of post-confluence inhibition of cell division. Two clones of mouse 3T3 fibroblasts that maintained post-confluence inhibition of cell division and low agglutinability differed substantially with respect to the surface distribution of concanavalin A. Patching of concanavalin A binding sites is neither necessary nor sufficient to explain differences in agglutinability between cell lines.", "PMID": 954079}
{"id": "pubmed23n0032_890", "title": "Methyl labeling of HeLa cell hnRNA: a comparison with mRNA.", "content": "The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal \"cap\" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-labeled oligonucleotides (\"di-\" and \"tri-\" nucleotides) which are not found in mRNA. Some of the dinucleotides may be precursors to the 2'-0-methylated nucleotides in the cap structures. These results are compatible with internal methylation of hnRNA molecules (both m6Ap and 2'-0-methyl) followed by hnRNA cleavage and the addition of the cap structure to generate at least some of the HeLa cell mRNA. It also appears that some hnRNA molecules, which are longer than most mRNA molecules, contain cap structures suggesting the derivation of some mRNA molecules from the 5' regions of hnRNA.", "contents": "Methyl labeling of HeLa cell hnRNA: a comparison with mRNA. The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal \"cap\" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-labeled oligonucleotides (\"di-\" and \"tri-\" nucleotides) which are not found in mRNA. Some of the dinucleotides may be precursors to the 2'-0-methylated nucleotides in the cap structures. These results are compatible with internal methylation of hnRNA molecules (both m6Ap and 2'-0-methyl) followed by hnRNA cleavage and the addition of the cap structure to generate at least some of the HeLa cell mRNA. It also appears that some hnRNA molecules, which are longer than most mRNA molecules, contain cap structures suggesting the derivation of some mRNA molecules from the 5' regions of hnRNA.", "PMID": 954080}
{"id": "pubmed23n0032_891", "title": "The size of pulse-labeled fibroin messenger RNA.", "content": "A method has been developed for the isolation of fibroin gene transcripts from total RNA of the silkworm Bombyx mori. It is based on affinity chromatography using Sephadex-bound polynucleotides capable of selectively hybridizing with fibroin mRNA sequences. In vivo pulse labeling of the posterior silk gland for periods of 10-35 min produces labeled heterogeneous nuclear RNA of high molecular weight (greater 40S). Fibroin gene transcripts can be selected from the total hnRNA population by two consecutive passages through the affinity column. Analysis of the column-bound material in denaturing polyacrylamide-agarose gels reveals that the size of pulse-labeled fibroin mRNA is essentially the same (within 5%) as that of mature cytoplasmic mRNA. This holds true for pulses as short as 6 min, where even nascent mRNA can be observed. However, a small shoulder of material is present on the heavy side of the pulse-labeled mRNA, which could be indicative of an extremely short-lived precurosr species. The purified pulse-labeled mRNA (10 min incorporation) has been further analyzed by chromatography in oligo(dT)-cellulose. The data show that the mRNA is polyadenylated within a few minutes after synthesis.", "contents": "The size of pulse-labeled fibroin messenger RNA. A method has been developed for the isolation of fibroin gene transcripts from total RNA of the silkworm Bombyx mori. It is based on affinity chromatography using Sephadex-bound polynucleotides capable of selectively hybridizing with fibroin mRNA sequences. In vivo pulse labeling of the posterior silk gland for periods of 10-35 min produces labeled heterogeneous nuclear RNA of high molecular weight (greater 40S). Fibroin gene transcripts can be selected from the total hnRNA population by two consecutive passages through the affinity column. Analysis of the column-bound material in denaturing polyacrylamide-agarose gels reveals that the size of pulse-labeled fibroin mRNA is essentially the same (within 5%) as that of mature cytoplasmic mRNA. This holds true for pulses as short as 6 min, where even nascent mRNA can be observed. However, a small shoulder of material is present on the heavy side of the pulse-labeled mRNA, which could be indicative of an extremely short-lived precurosr species. The purified pulse-labeled mRNA (10 min incorporation) has been further analyzed by chromatography in oligo(dT)-cellulose. The data show that the mRNA is polyadenylated within a few minutes after synthesis.", "PMID": 954081}
{"id": "pubmed23n0032_892", "title": "Analysis of the complexity and diversity of mRNA from chicken liver and oviduct.", "content": "We have analyzed the sequence complexity and diversity of poly(A)-containing mRNA derived from two highly differentiated chicken tissues. Two independent approaches were used in our analyses. The first involves the annealing of cDNA copies of mRNA to a vast excess of the template RNA; the second procedure uses hybridization between highly radioactive single-copy genomic DNA and mRNA. The results obtained using these two experimental approaches are in good accord and reveal the presence of 12,000-15,000 diverse mRNA species in both chicken liver and oviduct. In both cell types, the kinetics of annealing of cDNA to its template mRNA demonstrate discrete frequency classes with most of the different mRNA species present in fewer than 10 copies per cell. 70% of oviduct mRNA, however, consists of about 10 abundant RNA species, which probably are responsible for the synthesis of the egg white proteins. The diversity of mRNA species in chicken liver and oviduct was further studied by heterologous annealing reactions between cDNA or singlecopy genomic DNA and a vast excess of mRNA. These studies demonstrate that 85% of the different mRNA sequences detected are present in both liver and oviduct, and suggest that the vast majority of the information expressed as mRNA is required for the maintenance of cellular functions common to all tissues.", "contents": "Analysis of the complexity and diversity of mRNA from chicken liver and oviduct. We have analyzed the sequence complexity and diversity of poly(A)-containing mRNA derived from two highly differentiated chicken tissues. Two independent approaches were used in our analyses. The first involves the annealing of cDNA copies of mRNA to a vast excess of the template RNA; the second procedure uses hybridization between highly radioactive single-copy genomic DNA and mRNA. The results obtained using these two experimental approaches are in good accord and reveal the presence of 12,000-15,000 diverse mRNA species in both chicken liver and oviduct. In both cell types, the kinetics of annealing of cDNA to its template mRNA demonstrate discrete frequency classes with most of the different mRNA species present in fewer than 10 copies per cell. 70% of oviduct mRNA, however, consists of about 10 abundant RNA species, which probably are responsible for the synthesis of the egg white proteins. The diversity of mRNA species in chicken liver and oviduct was further studied by heterologous annealing reactions between cDNA or singlecopy genomic DNA and a vast excess of mRNA. These studies demonstrate that 85% of the different mRNA sequences detected are present in both liver and oviduct, and suggest that the vast majority of the information expressed as mRNA is required for the maintenance of cellular functions common to all tissues.", "PMID": 954082}
{"id": "pubmed23n0032_893", "title": "Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA.", "content": "The relationship between cell proliferation and the amount and diversity of polyribosome-associated poly(A)-containing messenger RNA [poly(A)+mRNA]has been investigated using a cloned AKR-mouse embryo cell culture system. The following results were obtained. First, an early response to the stimulation of proliferation of AKR-2B cells in culture is a rapid increase in the rate of accumulation of polyribosomal poly(A)+ mRNA. This results in a large increase in the total poly(A)+ mRNA content of rapidly proliferating cells compared to that found in resting cells. Second, the total amount of unique DNA sequence contributing to the poly(A)+ mRNA populations of both growing and resting cells is not detectably different. This corresponds to 9000-11,000 diverse gene equivalents of DNA and represents the transcription of 0.8-0.9% of the haploid mouse genome. Third, most of the increased poly(A)+ mRNA content of growing cells (greater than 90%) reflects an increased rate of production of polysomal mRNA species which are also found in resting cells. Fourth, growing cells appear to contain some species of poly(A)+ mRNA which are either absent or present in very low concentrations in non-growing cells. Within the limits of detection, however, all species of poly(A)+ mRNA present in non-growing cells are also present in growing cells.", "contents": "Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA. The relationship between cell proliferation and the amount and diversity of polyribosome-associated poly(A)-containing messenger RNA [poly(A)+mRNA]has been investigated using a cloned AKR-mouse embryo cell culture system. The following results were obtained. First, an early response to the stimulation of proliferation of AKR-2B cells in culture is a rapid increase in the rate of accumulation of polyribosomal poly(A)+ mRNA. This results in a large increase in the total poly(A)+ mRNA content of rapidly proliferating cells compared to that found in resting cells. Second, the total amount of unique DNA sequence contributing to the poly(A)+ mRNA populations of both growing and resting cells is not detectably different. This corresponds to 9000-11,000 diverse gene equivalents of DNA and represents the transcription of 0.8-0.9% of the haploid mouse genome. Third, most of the increased poly(A)+ mRNA content of growing cells (greater than 90%) reflects an increased rate of production of polysomal mRNA species which are also found in resting cells. Fourth, growing cells appear to contain some species of poly(A)+ mRNA which are either absent or present in very low concentrations in non-growing cells. Within the limits of detection, however, all species of poly(A)+ mRNA present in non-growing cells are also present in growing cells.", "PMID": 954083}
{"id": "pubmed23n0032_894", "title": "Chromatin structure visualization by immunoelectron microscopy.", "content": "Antibodies elicited in rabbits by chromatin and by purified histone H2B have been used to study the structure of chromatin by immunoelectron microscopy. Chromatin spread on grids reveals a structure of closely packed spherical particles with an average diameter of 104 A, arranged either in clusters or in linear arrays of beads, some of which have a supercoil-like arrangement. No DNA strings connecting the beads could be observed. Upon antibody binding, the diameter of the particles increases up to 300 A. This size is compatible with a model where one layer of gamma globulin molecules 110 A long encircles a sphere of chromatin 100 A in diameter. The presence of rabbit gamma globulins on the enlarged beads has been verified by the addition of ferritin-labeled goat anti-rabbit gamma globulins. Anti-chromatin sera which react with nonhistone proteins but not with free histones or DNA react with more than 95% of the beads; this suggests that most of the beads contain nonhistone proteins. Since the number of nonhistone proteins is large, it is improbable that each sphere contains a full complement of these proteins. We therefore suggest that the various chromatin spheres contain different types of nonhistone proteins. About 90% of the chromatin spheres reacted with antibodies to histone H2B, suggesting the most of the chromatin beads contain this type of histone.", "contents": "Chromatin structure visualization by immunoelectron microscopy. Antibodies elicited in rabbits by chromatin and by purified histone H2B have been used to study the structure of chromatin by immunoelectron microscopy. Chromatin spread on grids reveals a structure of closely packed spherical particles with an average diameter of 104 A, arranged either in clusters or in linear arrays of beads, some of which have a supercoil-like arrangement. No DNA strings connecting the beads could be observed. Upon antibody binding, the diameter of the particles increases up to 300 A. This size is compatible with a model where one layer of gamma globulin molecules 110 A long encircles a sphere of chromatin 100 A in diameter. The presence of rabbit gamma globulins on the enlarged beads has been verified by the addition of ferritin-labeled goat anti-rabbit gamma globulins. Anti-chromatin sera which react with nonhistone proteins but not with free histones or DNA react with more than 95% of the beads; this suggests that most of the beads contain nonhistone proteins. Since the number of nonhistone proteins is large, it is improbable that each sphere contains a full complement of these proteins. We therefore suggest that the various chromatin spheres contain different types of nonhistone proteins. About 90% of the chromatin spheres reacted with antibodies to histone H2B, suggesting the most of the chromatin beads contain this type of histone.", "PMID": 954084}
{"id": "pubmed23n0032_895", "title": "Quantitation of turnover and export to the cytoplasm of hnRNA transcribed in the Balbiani rings.", "content": "Some quantitative parameters of the intranuclear metabolism and export to the cytoplasm of Balbiani ring 1 and 2 RNA molecules in salivary gland cells of Chironomus tentans have been determined. Growing RNA chains in the Balbiani rings attain uniform labeling with RNA precursors after 20 min of incorporation. The specific activity of 75S RNA released from the Balbiani rings into the nuclear sap increases rapidly and reaches a maximum level between 90 and 180 min of labeling. After 20 min, labeled 75S RNA enters the cytoplasm and accumulates at a linear rate. However, only a small proportion of the RNA produced at the Balbiani ring loci can subsequently be recovered in the nuclear sap (14-17%) or cytoplasm (4-7%) as 75S RNA; presumably the remainder is degraded entirely. Experiments using inhibitors of elongation (actinomycin D) or initiation (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) revealed that no significant quanity of the 75S RNA transcribed can be chased into the cytoplasm. Both the kinetics of entry of labeled 75S RNA into the cytoplasm-that is, a constant rate of increase after a brief lag-and chase data are incompatible with a precursor-product relationship between the great majority of nuclear 75S RNA and cytoplasmic 75S RNA with messenger characteristics. The results are discussed in relation to the possibility that a post-transcriptional control mechanism is operating in these cells.", "contents": "Quantitation of turnover and export to the cytoplasm of hnRNA transcribed in the Balbiani rings. Some quantitative parameters of the intranuclear metabolism and export to the cytoplasm of Balbiani ring 1 and 2 RNA molecules in salivary gland cells of Chironomus tentans have been determined. Growing RNA chains in the Balbiani rings attain uniform labeling with RNA precursors after 20 min of incorporation. The specific activity of 75S RNA released from the Balbiani rings into the nuclear sap increases rapidly and reaches a maximum level between 90 and 180 min of labeling. After 20 min, labeled 75S RNA enters the cytoplasm and accumulates at a linear rate. However, only a small proportion of the RNA produced at the Balbiani ring loci can subsequently be recovered in the nuclear sap (14-17%) or cytoplasm (4-7%) as 75S RNA; presumably the remainder is degraded entirely. Experiments using inhibitors of elongation (actinomycin D) or initiation (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) revealed that no significant quanity of the 75S RNA transcribed can be chased into the cytoplasm. Both the kinetics of entry of labeled 75S RNA into the cytoplasm-that is, a constant rate of increase after a brief lag-and chase data are incompatible with a precursor-product relationship between the great majority of nuclear 75S RNA and cytoplasmic 75S RNA with messenger characteristics. The results are discussed in relation to the possibility that a post-transcriptional control mechanism is operating in these cells.", "PMID": 954085}
{"id": "pubmed23n0032_896", "title": "Appearance and disappearance of acetycholine receptor during differentiation of chick skeletal muscle in vitro.", "content": "During differentiation of embryonic chick skeletal muscle in culture, elaboration of acetylcholine receptor (AChR) and acetylcholinesterase occurs shortly after myoblast fusion. During further development, AChR was found to decrease markedly on the myotube surface, while acetylcholinesterase continued to increase. Surface distribution of AChR, as followed by autoradiography using 125I-alpha-bungarotoxin, was homogeneous in newly fused myotubes. With further differentiation, clusters of AChR appeared on the surface of the myotubes, and their subsequent disappearance paralleled a decrease in overall AChR levels. Quantitative autoradiography showed a reduction of over 75% in the density of AChR on the surface of well differentiated, cross-striated myotubes. Thus the appearance of AChR on the cell surface, its condensation into clusters, and finally its depletion seem to be sequential events in the differentiation of skeletal muscle in culture in the absence of direct neuronal influence.", "contents": "Appearance and disappearance of acetycholine receptor during differentiation of chick skeletal muscle in vitro. During differentiation of embryonic chick skeletal muscle in culture, elaboration of acetylcholine receptor (AChR) and acetylcholinesterase occurs shortly after myoblast fusion. During further development, AChR was found to decrease markedly on the myotube surface, while acetylcholinesterase continued to increase. Surface distribution of AChR, as followed by autoradiography using 125I-alpha-bungarotoxin, was homogeneous in newly fused myotubes. With further differentiation, clusters of AChR appeared on the surface of the myotubes, and their subsequent disappearance paralleled a decrease in overall AChR levels. Quantitative autoradiography showed a reduction of over 75% in the density of AChR on the surface of well differentiated, cross-striated myotubes. Thus the appearance of AChR on the cell surface, its condensation into clusters, and finally its depletion seem to be sequential events in the differentiation of skeletal muscle in culture in the absence of direct neuronal influence.", "PMID": 954086}
{"id": "pubmed23n0032_897", "title": "Low molecular weight viral RNAs transcribed by RNA polymerase III during adenovirus 2 infection.", "content": "Nuclei isolated from human cells productively infected with adenovirus 2 have been shown to synthesize four low molecular weight RNA species which hybridize efficiently to viral DNA. One species corresponds to the 5.5S or VA RNA (Ohe, Weissman, and Cooke, 1969), and is designated V156. The other three species are novel and have been designated V200, V140, V130, since they are approximately 200, 140, and 130 nucleotides in length, respectively. These viral RNAs retain their distinct electrophoretic properties after denaturation with formamide. RNA species with electrophoretic mobilities similar to those of the V200, V156, and V140 RNAs have been found in the cytoplasmic fraction of cells at late times after adenovirus infection. In isolated nuclei, the V200, V156, V140, and V130 RNAs are all synthesized by DNA-dependent RNA polymerase III, since synthesis is sensitive to high but not to low concentrations of alpha-amanitin. The synthesis of these low molecular weight RNAs continues for a prolonged period of time in isolated nuclei, suggesting that reinitiation occurs. Adenovirus 2 DNA fragments obtained by digestion with restriction endonucleases Eco RI and Sma I were used to map the location of the DNA sequences which encode the RNAs. All the low molecular weight RNAs hybridized to a region of the genome between o.18 and 0.38 fractional lengths from the left end of the adenovirus genome, suggesting that the respective DNA sequences are clustered. Other nonviral low molecular weight RNAs are synthesized in nuclei isolated from infected cells. These include the cellular 5S rRNA species which was minitored by its hybridization to purified 5S DNA from Xenopus laevis.", "contents": "Low molecular weight viral RNAs transcribed by RNA polymerase III during adenovirus 2 infection. Nuclei isolated from human cells productively infected with adenovirus 2 have been shown to synthesize four low molecular weight RNA species which hybridize efficiently to viral DNA. One species corresponds to the 5.5S or VA RNA (Ohe, Weissman, and Cooke, 1969), and is designated V156. The other three species are novel and have been designated V200, V140, V130, since they are approximately 200, 140, and 130 nucleotides in length, respectively. These viral RNAs retain their distinct electrophoretic properties after denaturation with formamide. RNA species with electrophoretic mobilities similar to those of the V200, V156, and V140 RNAs have been found in the cytoplasmic fraction of cells at late times after adenovirus infection. In isolated nuclei, the V200, V156, V140, and V130 RNAs are all synthesized by DNA-dependent RNA polymerase III, since synthesis is sensitive to high but not to low concentrations of alpha-amanitin. The synthesis of these low molecular weight RNAs continues for a prolonged period of time in isolated nuclei, suggesting that reinitiation occurs. Adenovirus 2 DNA fragments obtained by digestion with restriction endonucleases Eco RI and Sma I were used to map the location of the DNA sequences which encode the RNAs. All the low molecular weight RNAs hybridized to a region of the genome between o.18 and 0.38 fractional lengths from the left end of the adenovirus genome, suggesting that the respective DNA sequences are clustered. Other nonviral low molecular weight RNAs are synthesized in nuclei isolated from infected cells. These include the cellular 5S rRNA species which was minitored by its hybridization to purified 5S DNA from Xenopus laevis.", "PMID": 954087}
{"id": "pubmed23n0032_898", "title": "A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2.", "content": "A virus-coded low molecular weight RNA (5.2S), which migrates slightly faster on polyacrylamide gels than the well characterized adenovirus-specific 5.5S RNA, has been isolated from cells infected with adenovirus type 2. Hybridization-competition experiments and RNA fingerprints indicate that the two virus-associated (VA) RNAs differ in their primary structures. The gene for 5.2S RNA is located to the right of the gene for 5.5S RNA, on the I strand of a DNA segment which extends between positions 30.3 and 32.2 on the map of adenovirus type 2 DNA. Both 5.5S and 5.2S RNA can be detected early after infection and also in the presence of cytosine-arabinoside or cycloheximide. After the onset of viral DNA replication, the synthesis of 5.2S RNA levels off, whereas 5.5S RNA is synthesized in increasing amounts. Both 5.2S and 5.5S RNAs are synthesized in isolated nuclei by an enzyme which resembles RNA polymerase III in its sensitivity to alpha-amanitin. In isolated nuclei, both RNA species are labeled with beta-32P-labeled GTP, which suggests that they are initiated at separate promotor sites.", "contents": "A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2. A virus-coded low molecular weight RNA (5.2S), which migrates slightly faster on polyacrylamide gels than the well characterized adenovirus-specific 5.5S RNA, has been isolated from cells infected with adenovirus type 2. Hybridization-competition experiments and RNA fingerprints indicate that the two virus-associated (VA) RNAs differ in their primary structures. The gene for 5.2S RNA is located to the right of the gene for 5.5S RNA, on the I strand of a DNA segment which extends between positions 30.3 and 32.2 on the map of adenovirus type 2 DNA. Both 5.5S and 5.2S RNA can be detected early after infection and also in the presence of cytosine-arabinoside or cycloheximide. After the onset of viral DNA replication, the synthesis of 5.2S RNA levels off, whereas 5.5S RNA is synthesized in increasing amounts. Both 5.2S and 5.5S RNAs are synthesized in isolated nuclei by an enzyme which resembles RNA polymerase III in its sensitivity to alpha-amanitin. In isolated nuclei, both RNA species are labeled with beta-32P-labeled GTP, which suggests that they are initiated at separate promotor sites.", "PMID": 954088}
{"id": "pubmed23n0032_899", "title": "A direct estimate of the number of human gamma-globin genes.", "content": "The number of genes specifying human gamma-globin has been determined directly by hybridization of complementary DNA to total human DNA. The complementary DNA was enriched in sequences specific for gamma-globin genes by transcribing globin mRNA isolated from fetal reticulocytes with viral reverse transcriptase, and collecting the material which does not back-hybridize to adult globin mRNA. When hybridized in cDNA excess to DNA, very similar values are found for gamma-gene number as for beta-gene number, suggesting two or at most three gamma-globin genes per haploid human genome. This indicates that the non-Mendelian ratios of gamma-chain mutants found in heterozygotes are due to transcriptional or post-transcriptional regulation rather than to gene dosage. The number of each major human globin gene has now been determined directly by molecular methods.", "contents": "A direct estimate of the number of human gamma-globin genes. The number of genes specifying human gamma-globin has been determined directly by hybridization of complementary DNA to total human DNA. The complementary DNA was enriched in sequences specific for gamma-globin genes by transcribing globin mRNA isolated from fetal reticulocytes with viral reverse transcriptase, and collecting the material which does not back-hybridize to adult globin mRNA. When hybridized in cDNA excess to DNA, very similar values are found for gamma-gene number as for beta-gene number, suggesting two or at most three gamma-globin genes per haploid human genome. This indicates that the non-Mendelian ratios of gamma-chain mutants found in heterozygotes are due to transcriptional or post-transcriptional regulation rather than to gene dosage. The number of each major human globin gene has now been determined directly by molecular methods.", "PMID": 954089}
{"id": "pubmed23n0032_900", "title": "Small RNA species of the HeLa cell: metabolism and subcellular localization.", "content": "The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions. SnA is located in the nucleolus and is partially bonded to nucleolar 28S RNA. SnD, the most abundant of the small nuclear RNAs, is partially released from the nucleus when the nuclear preparation is briefly warmed. SnF is released from the nuclei when chromatin is digested with the micrococcal nuclease and not when pancreatic DNAase is used. The remainder of the small nuclear species remain in the nucleus following the digestion of chromatin and are concluded to be elements of the \"nuclear skeleton.\" SnK is found predominantly in the cytoplasm, but migrates quantitatively to the nuclear fraction in the presence of high levels of actinomycin D. ScL is totally cytoplasmic and is partially bound to cell membranes. It is the 7S RNA found in oncornavirus virions. All the small nuclear RNAs appear initially in the cytoplasmic fraction before fixation in the nucleus. Two short-lived cytoplasmic species behave kinetically as precursors to the stable nuclear RNAs.", "contents": "Small RNA species of the HeLa cell: metabolism and subcellular localization. The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions. SnA is located in the nucleolus and is partially bonded to nucleolar 28S RNA. SnD, the most abundant of the small nuclear RNAs, is partially released from the nucleus when the nuclear preparation is briefly warmed. SnF is released from the nuclei when chromatin is digested with the micrococcal nuclease and not when pancreatic DNAase is used. The remainder of the small nuclear species remain in the nucleus following the digestion of chromatin and are concluded to be elements of the \"nuclear skeleton.\" SnK is found predominantly in the cytoplasm, but migrates quantitatively to the nuclear fraction in the presence of high levels of actinomycin D. ScL is totally cytoplasmic and is partially bound to cell membranes. It is the 7S RNA found in oncornavirus virions. All the small nuclear RNAs appear initially in the cytoplasmic fraction before fixation in the nucleus. Two short-lived cytoplasmic species behave kinetically as precursors to the stable nuclear RNAs.", "PMID": 954090}
{"id": "pubmed23n0032_901", "title": "The effect of heat shock on RNA synthesis in Drosophila tissues.", "content": "Total RNA from Drosophila imaginal discs, labeled under conditions of heat shock, is analyzed by hybridization in situ to salivary gland polytene chromosomes. Grain densities over the hybridizing bands are compared, showing that the response to heat shock is similar for several disc types and fly stocks. Alteration of the culture medium used for labeling during heat shock results in the specific induction (in discs, salvary glands, or fat body) of one of the heat-shock loci to a level far beyond that normally seen. We discuss the implications of this specific induction regarding the mechanism of response. We also discuss the observed difference between transcription labeling of salivary gland chromosomes and labeling by in situ hybridization.", "contents": "The effect of heat shock on RNA synthesis in Drosophila tissues. Total RNA from Drosophila imaginal discs, labeled under conditions of heat shock, is analyzed by hybridization in situ to salivary gland polytene chromosomes. Grain densities over the hybridizing bands are compared, showing that the response to heat shock is similar for several disc types and fly stocks. Alteration of the culture medium used for labeling during heat shock results in the specific induction (in discs, salvary glands, or fat body) of one of the heat-shock loci to a level far beyond that normally seen. We discuss the implications of this specific induction regarding the mechanism of response. We also discuss the observed difference between transcription labeling of salivary gland chromosomes and labeling by in situ hybridization.", "PMID": 954091}
{"id": "pubmed23n0032_902", "title": "The translational capacity of deadenylated ovalbumin messenger RNA.", "content": "We present evidence that the poly(A) sequence at the 3' end of ovalbumin mRNA has an effect on its translational efficiency in a reticulocyte lysate cell-free system. Polynucleotide phosphorylase has been used to remove selectively the poly(A) while leaving the rest of the molecule intact. It is shown that the stability of the mRNA in a cell free system is not appreciably affected by this procedure. Measurements of the size of ovalbumin-synthesizing polysomes, rate of peptide elongation, and number of rounds of translation per messenger show a generally reduced efficiency for deadenylated mRNA compared to native mRNA. No comparable difference was observed in experiments with a wheat germ cell-free system, which gives few rounds of translation per mRNA. This indicates that the effect results from a lowering of the efficiency of reinitiation on deadenylated mRNA.", "contents": "The translational capacity of deadenylated ovalbumin messenger RNA. We present evidence that the poly(A) sequence at the 3' end of ovalbumin mRNA has an effect on its translational efficiency in a reticulocyte lysate cell-free system. Polynucleotide phosphorylase has been used to remove selectively the poly(A) while leaving the rest of the molecule intact. It is shown that the stability of the mRNA in a cell free system is not appreciably affected by this procedure. Measurements of the size of ovalbumin-synthesizing polysomes, rate of peptide elongation, and number of rounds of translation per messenger show a generally reduced efficiency for deadenylated mRNA compared to native mRNA. No comparable difference was observed in experiments with a wheat germ cell-free system, which gives few rounds of translation per mRNA. This indicates that the effect results from a lowering of the efficiency of reinitiation on deadenylated mRNA.", "PMID": 954092}
{"id": "pubmed23n0032_903", "title": "The RNA polymerase II of an alpha-amanitin-resistant Chinese hamster ovary cell line.", "content": "Amal, an alpha-amanitin-resistant mutant of the Chinese hamster ovary cell line, contains an RNA polymerase activity which elutes from DEAE-Sephadex at a salt concentration characteristic of an RNA polymerase II, but which is not sensitive to alpha-amanitin at levels where the polymerase II of wild-type cells is strongly inhibited. This result suggests that Amal owes its amanitin-resistant phenotype to a mutation affecting one of its genes for RNA polymerase II. To test this hypothesis, we purified the enzyme from Amal and then compared its properties with those of the wild-type enzyme. The mutant enzyme is indeed a polymerase II, and is over 600 times less sensitive to alpha-amanitin and more thermolabile than the wild-type enzyme.", "contents": "The RNA polymerase II of an alpha-amanitin-resistant Chinese hamster ovary cell line. Amal, an alpha-amanitin-resistant mutant of the Chinese hamster ovary cell line, contains an RNA polymerase activity which elutes from DEAE-Sephadex at a salt concentration characteristic of an RNA polymerase II, but which is not sensitive to alpha-amanitin at levels where the polymerase II of wild-type cells is strongly inhibited. This result suggests that Amal owes its amanitin-resistant phenotype to a mutation affecting one of its genes for RNA polymerase II. To test this hypothesis, we purified the enzyme from Amal and then compared its properties with those of the wild-type enzyme. The mutant enzyme is indeed a polymerase II, and is over 600 times less sensitive to alpha-amanitin and more thermolabile than the wild-type enzyme.", "PMID": 954093}
{"id": "pubmed23n0032_904", "title": "Biochemical characterization of azetidine carboxylic acid-resistant Chinese hamster cells.", "content": "We have isolated variants of Chinese hamster lung (CHL) fibroblasts with decreased sensitivity to the toxic proline analog, L-azetidine-2-carboxylic acid (AZCA). Resistance to AZCA is a stable characteristic of these cell lines since it is maintained after 40 generations in nonselective medium. Two AZCA-resistant cell lines overproduce and excrete more proline than wild-type cells. Furthermore, AZCA rapidly inhibits the synthesis of proline from glutamic acid in wild-type but not the resistant cell lines. These studies suggest that resistance to AZCA in these cell lines is due to an alteration in some component of the proline biosynthetic pathway, possibly a regulatory site that is inhibited by AZCA.", "contents": "Biochemical characterization of azetidine carboxylic acid-resistant Chinese hamster cells. We have isolated variants of Chinese hamster lung (CHL) fibroblasts with decreased sensitivity to the toxic proline analog, L-azetidine-2-carboxylic acid (AZCA). Resistance to AZCA is a stable characteristic of these cell lines since it is maintained after 40 generations in nonselective medium. Two AZCA-resistant cell lines overproduce and excrete more proline than wild-type cells. Furthermore, AZCA rapidly inhibits the synthesis of proline from glutamic acid in wild-type but not the resistant cell lines. These studies suggest that resistance to AZCA in these cell lines is due to an alteration in some component of the proline biosynthetic pathway, possibly a regulatory site that is inhibited by AZCA.", "PMID": 954094}
{"id": "pubmed23n0032_905", "title": "Insulin receptors in normal and transformed fibroblasts: relationship to growth and transformation.", "content": "The insulin receptors in normal and transformed lines of mouse Balb/3t3 fibroblasts have been studied. In the normal fibroblasts, the binding of insulin was low in growing cells and increased 2-9 fold in confluent stationary cells. Insulin binding was increased whether growth arrest was due to contact inhibition of growth or serum starvation. When serum-starved cells were stimulated to grow by the addition of fresh serum, insulin binding declined. In cells transformed by simian virus 40, Kirsten, Moloney, and Harvey sarcoma viruses, methylcholanthrene, X rays, or spontaneously, the binding was low, in the same range as growing normal cells. In simian virus 40-transformed cells, insulin binding increased 4 fold as the cells reached higher densities in culture. No relationship to changes in cell size was found. The differences in binding were due to changes in the concentration of the receptors, without changes in their affinity for the hormone.", "contents": "Insulin receptors in normal and transformed fibroblasts: relationship to growth and transformation. The insulin receptors in normal and transformed lines of mouse Balb/3t3 fibroblasts have been studied. In the normal fibroblasts, the binding of insulin was low in growing cells and increased 2-9 fold in confluent stationary cells. Insulin binding was increased whether growth arrest was due to contact inhibition of growth or serum starvation. When serum-starved cells were stimulated to grow by the addition of fresh serum, insulin binding declined. In cells transformed by simian virus 40, Kirsten, Moloney, and Harvey sarcoma viruses, methylcholanthrene, X rays, or spontaneously, the binding was low, in the same range as growing normal cells. In simian virus 40-transformed cells, insulin binding increased 4 fold as the cells reached higher densities in culture. No relationship to changes in cell size was found. The differences in binding were due to changes in the concentration of the receptors, without changes in their affinity for the hormone.", "PMID": 954096}
{"id": "pubmed23n0032_906", "title": "Kinetics of formation of 5' terminal caps in mRNA.", "content": "A kinetic analysis of the labeling of the methylated components of messenger RNA and heterogeneous nuclear RNA in mouse L cells indicates that the 5' terminal cap I structures (m7GpppXmpYp) of mRNA are derived from 5' terminal cap structures of hnRNA. Most of the hnRNA caps are conserved during processing, whereas only a portion of the internal m6A residues in hnRNA are conserved. The  cap II structures (m7GpppXmpYmpZp), which constitute the 5' termini of some mRNAs, arise by a \"secondary\" methylation that occurs after the mRNAs have entered the cytoplasm. This secondary methylation is apparently restricted to a particular subclass of mRNAs having a high frequency of pyrimidine nucleotides at position Y, a composition at position X which differs from that of the bulk of the cap I-terminated mRNAs, and a relatively slow rate of turnover.", "contents": "Kinetics of formation of 5' terminal caps in mRNA. A kinetic analysis of the labeling of the methylated components of messenger RNA and heterogeneous nuclear RNA in mouse L cells indicates that the 5' terminal cap I structures (m7GpppXmpYp) of mRNA are derived from 5' terminal cap structures of hnRNA. Most of the hnRNA caps are conserved during processing, whereas only a portion of the internal m6A residues in hnRNA are conserved. The  cap II structures (m7GpppXmpYmpZp), which constitute the 5' termini of some mRNAs, arise by a \"secondary\" methylation that occurs after the mRNAs have entered the cytoplasm. This secondary methylation is apparently restricted to a particular subclass of mRNAs having a high frequency of pyrimidine nucleotides at position Y, a composition at position X which differs from that of the bulk of the cap I-terminated mRNAs, and a relatively slow rate of turnover.", "PMID": 954097}
{"id": "pubmed23n0032_907", "title": "A reinvestigation of 5' leads to 3' polarity in 40S ribosomal RNA precursor of Xenopus laevis.", "content": "The 5' leads to 3' polarity of the 40S precursor rRNA molecule relative to the location of the 18S and 28S RNA regions in the precursor has been reinvestigated. Fragments of rDNA derived by the restriction endonuclease EcoRI and cloned in E. coli were partially digested with the exonuclease induced by bacteriophage  lambda and with exonuclease III from E. coli. The resulting rDNA fragments with single-stranded tails were hybridized separately with 18S and 28S rRNA, and the formation of the hybrid was monitored by determination of radioactivity and by electron microscopy. Since the location of the EcoRI sites in rDNA is known, and the specificity of the two exonucleases for 5' and 3' ends of DNA strands has been established, the hybridization of the different partially digested rDNA fragments with either 18S or 28S rRNA could be interpreted in terms of polarity of the coding strand of rDNA, and consequently of the RNA (see models in Figure 1). The results supports the following model for the rRNA precursor moleculre: 5' end-transcribed space-18S gene-transcribed space-28S gene-3' end.", "contents": "A reinvestigation of 5' leads to 3' polarity in 40S ribosomal RNA precursor of Xenopus laevis. The 5' leads to 3' polarity of the 40S precursor rRNA molecule relative to the location of the 18S and 28S RNA regions in the precursor has been reinvestigated. Fragments of rDNA derived by the restriction endonuclease EcoRI and cloned in E. coli were partially digested with the exonuclease induced by bacteriophage  lambda and with exonuclease III from E. coli. The resulting rDNA fragments with single-stranded tails were hybridized separately with 18S and 28S rRNA, and the formation of the hybrid was monitored by determination of radioactivity and by electron microscopy. Since the location of the EcoRI sites in rDNA is known, and the specificity of the two exonucleases for 5' and 3' ends of DNA strands has been established, the hybridization of the different partially digested rDNA fragments with either 18S or 28S rRNA could be interpreted in terms of polarity of the coding strand of rDNA, and consequently of the RNA (see models in Figure 1). The results supports the following model for the rRNA precursor moleculre: 5' end-transcribed space-18S gene-transcribed space-28S gene-3' end.", "PMID": 954098}
{"id": "pubmed23n0032_908", "title": "Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.", "content": "The electrophoretic separation of labeled \"9S\" histone mRNAs obtained from cleaving sea urchin polysomes was found at first to be highly unreproducible. It became evident that the secondary structure of the individual mRNAs had a greater effect on their relative electrophoretic mobilities than did their molecular weight differentials. We determined the parameters affecting electrophoretic mobility by the novel method of running the labeled polysomal RNA in slab gels across polyacrylamide and urea gradients. The initially complex and species-specific electrophoretic pattern could then, by a judicious choice of denaturing conditions, be simplified to yield five well defined classes of labeled mRNAs. Using optimal conditions for the separation of the RNA components, five messengers were isolated from Psammechinus embryos by preparative disc electrophoresis, four of which, after two electrophoretic separations, exhibited a unimodal distribution. Each of the mRNAs was translated in vitro, four of the five fractions promoting the synthesis of one major protein. The in vitro products were characterized by comparison of their electrophoretic mobilities with those of known sea urchin histones. It was thus possible to correlate individual mRNAs with specific histones. We propose that the five mRNAs designated a-e in order of decreasing electrophoretic mobility code for the histones H4, H2A, H2B, H3, and H1.", "contents": "Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs. The electrophoretic separation of labeled \"9S\" histone mRNAs obtained from cleaving sea urchin polysomes was found at first to be highly unreproducible. It became evident that the secondary structure of the individual mRNAs had a greater effect on their relative electrophoretic mobilities than did their molecular weight differentials. We determined the parameters affecting electrophoretic mobility by the novel method of running the labeled polysomal RNA in slab gels across polyacrylamide and urea gradients. The initially complex and species-specific electrophoretic pattern could then, by a judicious choice of denaturing conditions, be simplified to yield five well defined classes of labeled mRNAs. Using optimal conditions for the separation of the RNA components, five messengers were isolated from Psammechinus embryos by preparative disc electrophoresis, four of which, after two electrophoretic separations, exhibited a unimodal distribution. Each of the mRNAs was translated in vitro, four of the five fractions promoting the synthesis of one major protein. The in vitro products were characterized by comparison of their electrophoretic mobilities with those of known sea urchin histones. It was thus possible to correlate individual mRNAs with specific histones. We propose that the five mRNAs designated a-e in order of decreasing electrophoretic mobility code for the histones H4, H2A, H2B, H3, and H1.", "PMID": 954099}
{"id": "pubmed23n0032_909", "title": "Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.", "content": "Histone DNA of Psammechinus miliaris was obtained in an enriched form by buoyant density gradient centrifugation and was cleaved into 6 kb repeat units (Birnstiel et al., 1975a) by the action of the specific endonucleases EcoRI and HindIII. Since it was suspected that the 6 kb unit harbored all five histone-coding sequences, the histone DNA unit was subdivided into five segments with the aim of providing five fragments carrying just one coding sequence each. This was achieved by the combined use of EcoRI Hindll, Hindlll, and Hpa I. A physical map was constructed from the overlaps arising in these restriction experiments. Each of the five segments was shown to hybridize uniquely with just one of the five highly purified histone mRNAs (Gross et al., 1976a). By this procedure, the order of the mRNA sequences on the histone DNA was found to be a, c, d, b, e (Gross et al., 1976a), and hence of the protein coding sequences H4, H2B, H3, H2A, and H1. Further evidence is presented that the 6 kb repeat unit, amplified by means of a Murray lambda vector phage, contains AT-rich DNA sequences which would be expected not to code for histone proteins.", "contents": "Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences. Histone DNA of Psammechinus miliaris was obtained in an enriched form by buoyant density gradient centrifugation and was cleaved into 6 kb repeat units (Birnstiel et al., 1975a) by the action of the specific endonucleases EcoRI and HindIII. Since it was suspected that the 6 kb unit harbored all five histone-coding sequences, the histone DNA unit was subdivided into five segments with the aim of providing five fragments carrying just one coding sequence each. This was achieved by the combined use of EcoRI Hindll, Hindlll, and Hpa I. A physical map was constructed from the overlaps arising in these restriction experiments. Each of the five segments was shown to hybridize uniquely with just one of the five highly purified histone mRNAs (Gross et al., 1976a). By this procedure, the order of the mRNA sequences on the histone DNA was found to be a, c, d, b, e (Gross et al., 1976a), and hence of the protein coding sequences H4, H2B, H3, H2A, and H1. Further evidence is presented that the 6 kb repeat unit, amplified by means of a Murray lambda vector phage, contains AT-rich DNA sequences which would be expected not to code for histone proteins.", "PMID": 954100}
{"id": "pubmed23n0032_910", "title": "Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.", "content": "Fragments of histone DNA produced by restriction endonucleases contain 5' and 3' termini with defined topologies relative to the histone-coding sequences. After limited resection with lambda-exonuclease, the 6 kb Hindlll histone DNA fragment (see Schaffner et al., 1976) hybridizes to H4 histone mRNA whether or not the DNA has been denatured. This shown that the coding sequence for the histone H4 is proximal to the 3' terminus of the Hindlll restriction fragment. By contrast, lambda-exonuclease digestion of the 6 kb EcoRI histone DNA fragment drastically reduces hybridization of the H4 mRNA. Hence in this molecule, the H4 DNA sequence is near a 5' terminus of the EcoRI restriction fragment. From these results and those described in the preceding paper (Schaffner et al., 1976), the polarity of the H4 gene is therefore 5' H2B leads to H4 leads to H1 3'. Cloned Psammechinus histone DNA (S. G. Clarkson, H. Smith, W. Schaffner, K. Gross, and M. Birnstiel, manuscript submitted for publication) may be strand-separated by electrophoresis. Highly purified histone mRNAs (Gross et al., 1976) all hybridize almost exclusively to the strand of lesser electrophoretic mobility. It follows that all coding sequences are arranged in tandem within the same DNA strand, and that hence they share the same polarity as the H4 DNA sequences. Transcription therefore proceeds in the gene cluster in the direction H4 leads to H2B leads to H3 leads to H2A leads to H1.", "contents": "Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences. Fragments of histone DNA produced by restriction endonucleases contain 5' and 3' termini with defined topologies relative to the histone-coding sequences. After limited resection with lambda-exonuclease, the 6 kb Hindlll histone DNA fragment (see Schaffner et al., 1976) hybridizes to H4 histone mRNA whether or not the DNA has been denatured. This shown that the coding sequence for the histone H4 is proximal to the 3' terminus of the Hindlll restriction fragment. By contrast, lambda-exonuclease digestion of the 6 kb EcoRI histone DNA fragment drastically reduces hybridization of the H4 mRNA. Hence in this molecule, the H4 DNA sequence is near a 5' terminus of the EcoRI restriction fragment. From these results and those described in the preceding paper (Schaffner et al., 1976), the polarity of the H4 gene is therefore 5' H2B leads to H4 leads to H1 3'. Cloned Psammechinus histone DNA (S. G. Clarkson, H. Smith, W. Schaffner, K. Gross, and M. Birnstiel, manuscript submitted for publication) may be strand-separated by electrophoresis. Highly purified histone mRNAs (Gross et al., 1976) all hybridize almost exclusively to the strand of lesser electrophoretic mobility. It follows that all coding sequences are arranged in tandem within the same DNA strand, and that hence they share the same polarity as the H4 DNA sequences. Transcription therefore proceeds in the gene cluster in the direction H4 leads to H2B leads to H3 leads to H2A leads to H1.", "PMID": 954101}
{"id": "pubmed23n0032_911", "title": "Biosynthesis and stability of globin mRNA in cultured erythroleukemic Friend cells.", "content": "Biosynthesis and stability of the mRNA population in DMSO-induced Friend erythroleukemic cells were studied after labeling the RNA with 3H-uridine and then chasing it with nonlabeled uridine. Globin RNA metabolism was studied by hybridization to excess complementary DNA convalently coupled to oligo(dT)-cellulose. After a labeling period of 120 min, 2-4% of the poly(A)-containing labeled RNA was in globin RNA; it decayed with a half-life of 16-17 hr. The rest of the poly(A)-containing RNA was composed to two kinetic populations: 85-90% decayed with a half-life of about 3 hr, while 10% decayed with a half-life of about 37 hr. The portion of globin RNA in labeled poly(A)-containing RNA behaved in an unexpected fashion during the chase period. During the initial chase period, the percentage of globin RNA increased rapidly, reaching a maximum of about 15% at 20 hr, but it subsequently declined gradually. Based on these findings, a model was built that describes the changes in the proportion of globin mRNA in poly(A)-containing RNA during continuous synthesis and after chase of the labeled RNA. It appears that if the parameters described remain constant during the maturation of erythroblasts, then this model would not account for the almost exclusive presence of globin RNA in the reticulocyte. By far the most effective way to achieve this high level of globin RNA is the destabilization of the mRNA population which is more stable than globin RNA, and not the stabilization of globin RNA itself.", "contents": "Biosynthesis and stability of globin mRNA in cultured erythroleukemic Friend cells. Biosynthesis and stability of the mRNA population in DMSO-induced Friend erythroleukemic cells were studied after labeling the RNA with 3H-uridine and then chasing it with nonlabeled uridine. Globin RNA metabolism was studied by hybridization to excess complementary DNA convalently coupled to oligo(dT)-cellulose. After a labeling period of 120 min, 2-4% of the poly(A)-containing labeled RNA was in globin RNA; it decayed with a half-life of 16-17 hr. The rest of the poly(A)-containing RNA was composed to two kinetic populations: 85-90% decayed with a half-life of about 3 hr, while 10% decayed with a half-life of about 37 hr. The portion of globin RNA in labeled poly(A)-containing RNA behaved in an unexpected fashion during the chase period. During the initial chase period, the percentage of globin RNA increased rapidly, reaching a maximum of about 15% at 20 hr, but it subsequently declined gradually. Based on these findings, a model was built that describes the changes in the proportion of globin mRNA in poly(A)-containing RNA during continuous synthesis and after chase of the labeled RNA. It appears that if the parameters described remain constant during the maturation of erythroblasts, then this model would not account for the almost exclusive presence of globin RNA in the reticulocyte. By far the most effective way to achieve this high level of globin RNA is the destabilization of the mRNA population which is more stable than globin RNA, and not the stabilization of globin RNA itself.", "PMID": 954102}
{"id": "pubmed23n0032_912", "title": "Globin mRNA sequences in polyadenylated and nonpolyadenylated nuclear precursor-messenger RNA from avian erythroblasts.", "content": "Nuclear RNA from immature duck erythrocytes was fractionated into polyadenylated and nonpolyadenylated fractions, and globin mRNA sequences were determined by hybridization to DNA complementary to globin mRNA. 80--90% of labeled nuclear RNA is found to be nonpolyadenylated, and 70--80% of the globin mRNA sequences present in the nucleus are found in nonpolyadenylated molecules. These data suggest that polyadenylation does not specifically select for globin mRNA sequences. The nonpolyadenylated globin mRNA sequences present in the nucleus are found mostly in molecules of small size, close to the size of polyribosomal globin mRNA, suggesting that polyadenylation is a later event in globin mRNA formation.", "contents": "Globin mRNA sequences in polyadenylated and nonpolyadenylated nuclear precursor-messenger RNA from avian erythroblasts. Nuclear RNA from immature duck erythrocytes was fractionated into polyadenylated and nonpolyadenylated fractions, and globin mRNA sequences were determined by hybridization to DNA complementary to globin mRNA. 80--90% of labeled nuclear RNA is found to be nonpolyadenylated, and 70--80% of the globin mRNA sequences present in the nucleus are found in nonpolyadenylated molecules. These data suggest that polyadenylation does not specifically select for globin mRNA sequences. The nonpolyadenylated globin mRNA sequences present in the nucleus are found mostly in molecules of small size, close to the size of polyribosomal globin mRNA, suggesting that polyadenylation is a later event in globin mRNA formation.", "PMID": 954103}
{"id": "pubmed23n0032_913", "title": "Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cells.", "content": "The role of heme in erythroid development is investigated in erythroleukemic (Friend) cells. Exogenous hemin induces the accumulation of globin mRNA and globin protein in T3-C12 erythroleukemia cells to levels comparable to those induced by polar solvents, such as dimethylsulfoxide (DMSO). The hemin concentration required for maximal induction (10(-4) M) is the same as that which stimulates globin message translation in reticulocytes or cell-free reticulocyte lysates. Hemin and DMSO together cause T3-C12 cells to accumulate 8-9 fold more globin mRNA than either inducer individually. The kinetics of globin mRNA induction in hemin as compared to DMSO are very different: globin message accumulation begins 4 hr after hemin addition, but not until 30--40 hr after DMSO addition. Biliverdin induces 20--40 fold less hemoglobin than hemin; delta-aminolevulinic acid and porphobilinogen do not induce.", "contents": "Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cells. The role of heme in erythroid development is investigated in erythroleukemic (Friend) cells. Exogenous hemin induces the accumulation of globin mRNA and globin protein in T3-C12 erythroleukemia cells to levels comparable to those induced by polar solvents, such as dimethylsulfoxide (DMSO). The hemin concentration required for maximal induction (10(-4) M) is the same as that which stimulates globin message translation in reticulocytes or cell-free reticulocyte lysates. Hemin and DMSO together cause T3-C12 cells to accumulate 8-9 fold more globin mRNA than either inducer individually. The kinetics of globin mRNA induction in hemin as compared to DMSO are very different: globin message accumulation begins 4 hr after hemin addition, but not until 30--40 hr after DMSO addition. Biliverdin induces 20--40 fold less hemoglobin than hemin; delta-aminolevulinic acid and porphobilinogen do not induce.", "PMID": 954104}
{"id": "pubmed23n0032_914", "title": "Tissue-specific forms of actin in the developing chick.", "content": "Actin has been identified in nonmuscle and muscle tissues as a highly conserved homogeneous protein. We have identified and characterized actin from embryonic and adult chick brain and muscle, and have compared these actins by SDS and urea/SDS gradient polyacrylamide gel electrophoresis. In the presence of SDS alone, embryonic or adult brain and muscle actin co-migrate as homogeneous polypeptides. Electrophoresis of both actins in the presence of urea and SDS, however, reveals that brain and muscle actins migrate with distinctly different mobilities. Actin from embryonic thigh muscle at different stages of development migrates as two separate components. In early muscle development, only the \"brain\" type actin is present. As muscle development progresses the \"muscle\" type actin becomes relatively more abundant, so that by day 20 of embryonic development, \"muscle\" actin becomes predominant. These results may be interpreted as due to differences in the primary structure of actin.", "contents": "Tissue-specific forms of actin in the developing chick. Actin has been identified in nonmuscle and muscle tissues as a highly conserved homogeneous protein. We have identified and characterized actin from embryonic and adult chick brain and muscle, and have compared these actins by SDS and urea/SDS gradient polyacrylamide gel electrophoresis. In the presence of SDS alone, embryonic or adult brain and muscle actin co-migrate as homogeneous polypeptides. Electrophoresis of both actins in the presence of urea and SDS, however, reveals that brain and muscle actins migrate with distinctly different mobilities. Actin from embryonic thigh muscle at different stages of development migrates as two separate components. In early muscle development, only the \"brain\" type actin is present. As muscle development progresses the \"muscle\" type actin becomes relatively more abundant, so that by day 20 of embryonic development, \"muscle\" actin becomes predominant. These results may be interpreted as due to differences in the primary structure of actin.", "PMID": 954105}
{"id": "pubmed23n0032_915", "title": "Evidence for the formation of hybrid DNA during mitotic recombination in Chinese hamster cells.", "content": "Direct evidence is provided for the formation of hybrid DNA during mitotic recombination in CHO cells. The cells were labeled for one round of replication in medium containing BUdR, so that the density of the DNA was heavy light (HL) and then returned to light medium. Further DNA synthesis, during either repair or chromosome replication, can only result in HL or fully light (LL) DNA; however, the formation of hybrid DNA as part of the process of recombinational repair will produce some fully heavy (HH) DNA. A small fraction of DNA containing regions of HH DNA has been detected on neutral CsC1 gradients, and the amount of this DNA is increased by treatment of the cells with mitomycin C. Increasing doses of mitomycin C produce smimlar increases in both the amount of HH DNA and the frequency of sister chromatid exchanges measured cytologically. This correlation provides evidence that the HH DNA is hybrid DNA, formed as an intermediate in recombinational repair.", "contents": "Evidence for the formation of hybrid DNA during mitotic recombination in Chinese hamster cells. Direct evidence is provided for the formation of hybrid DNA during mitotic recombination in CHO cells. The cells were labeled for one round of replication in medium containing BUdR, so that the density of the DNA was heavy light (HL) and then returned to light medium. Further DNA synthesis, during either repair or chromosome replication, can only result in HL or fully light (LL) DNA; however, the formation of hybrid DNA as part of the process of recombinational repair will produce some fully heavy (HH) DNA. A small fraction of DNA containing regions of HH DNA has been detected on neutral CsC1 gradients, and the amount of this DNA is increased by treatment of the cells with mitomycin C. Increasing doses of mitomycin C produce smimlar increases in both the amount of HH DNA and the frequency of sister chromatid exchanges measured cytologically. This correlation provides evidence that the HH DNA is hybrid DNA, formed as an intermediate in recombinational repair.", "PMID": 954106}
{"id": "pubmed23n0032_916", "title": "[Electron microscopy of hepatocytes in voluntary blood donors with positive finding of Australia antigen].", "content": "In twenty blood-donors without clinical symptoms of any hepatic lesion whatever there were ascertained positive serum findings of Australian antigen. In three samples of hepatobiopsy worked up in these carriers of HB Ag we found specific alterations; once it was the case of virus-like dense particles found only in the liver, and twice there were similar dense spherical corpuscles in the cisterns of smooth endoplasmatic reticule. Once we found a relatively great number of filamentous adjustments in a distended endoplasmatic reticule, simultaneously with less numerous spherical dense corpuscles which may be considered, however, as most likely the modified covering material of the virus.", "contents": "[Electron microscopy of hepatocytes in voluntary blood donors with positive finding of Australia antigen]. In twenty blood-donors without clinical symptoms of any hepatic lesion whatever there were ascertained positive serum findings of Australian antigen. In three samples of hepatobiopsy worked up in these carriers of HB Ag we found specific alterations; once it was the case of virus-like dense particles found only in the liver, and twice there were similar dense spherical corpuscles in the cisterns of smooth endoplasmatic reticule. Once we found a relatively great number of filamentous adjustments in a distended endoplasmatic reticule, simultaneously with less numerous spherical dense corpuscles which may be considered, however, as most likely the modified covering material of the virus.", "PMID": 954120}
{"id": "pubmed23n0032_917", "title": "[Clinically unrecognized valvular defects in high age groups].", "content": "The study has been based on an autopsy series of 474 unrecognized valvular defects of the left heart in high age groups beyond 65. The series comprised 186 males and 288 females with an average age of 77. The incidence of valvular defects was 4.02%. There was a significantly higher incidence of mitral valve insufficiency than in lower age groups of clinically recognized valvular defects (10.75%). The ratio of mitral insufficiency to the stenosis of the left venous ostium and combined mitral defect with prevalence of stenosis was 1:4. The prevalence of males in the series of isolated stenosis of the left arterial ostium attained only 1.35:1.", "contents": "[Clinically unrecognized valvular defects in high age groups]. The study has been based on an autopsy series of 474 unrecognized valvular defects of the left heart in high age groups beyond 65. The series comprised 186 males and 288 females with an average age of 77. The incidence of valvular defects was 4.02%. There was a significantly higher incidence of mitral valve insufficiency than in lower age groups of clinically recognized valvular defects (10.75%). The ratio of mitral insufficiency to the stenosis of the left venous ostium and combined mitral defect with prevalence of stenosis was 1:4. The prevalence of males in the series of isolated stenosis of the left arterial ostium attained only 1.35:1.", "PMID": 954121}
{"id": "pubmed23n0032_918", "title": "[Cytocarcinogenesis of the rat hepatoma induced by N-nitrosomorpholine].", "content": "In experiments carried out on Wistar rats ultrastructrual charges of hepatocytes were studied during long-term carcinogenesis due to N-Nitrosomorpholine administered as a 20 mg% solution in drinking water. Carcinoma usually developed after the 20th week of the experiment. The development of the tumour was preceded by early dystrophic changes and, later, by a precancerous posttoxic \"glycogenosis\". The structural alterations as observed during the precancerous stage were rather noncharacteristic so that they did not permit to disclose the exact point of neoplastic change of the hepatocytes. Nevertheless, the model of carcinogenesis as demonstrated here appears to be a rather standard one and seems to be suitable for studying oncological problems of general character.", "contents": "[Cytocarcinogenesis of the rat hepatoma induced by N-nitrosomorpholine]. In experiments carried out on Wistar rats ultrastructrual charges of hepatocytes were studied during long-term carcinogenesis due to N-Nitrosomorpholine administered as a 20 mg% solution in drinking water. Carcinoma usually developed after the 20th week of the experiment. The development of the tumour was preceded by early dystrophic changes and, later, by a precancerous posttoxic \"glycogenosis\". The structural alterations as observed during the precancerous stage were rather noncharacteristic so that they did not permit to disclose the exact point of neoplastic change of the hepatocytes. Nevertheless, the model of carcinogenesis as demonstrated here appears to be a rather standard one and seems to be suitable for studying oncological problems of general character.", "PMID": 954122}
{"id": "pubmed23n0032_919", "title": "The interaction of acetoxy-dimethylnitrosamine, a proximate metabolite of the carcinogenic amine, and bacteriophages R17 and T7.", "content": "The biological and physicochemical effects of reacting bacteriophages R17 and T7 with acetoxy-dimethylnitrosamine (ADMN) have been studied. The rate-determining step in the reactions appeared to be the loss of the acetoxy group by hydrolysis, the hydroxymethyl-methylnitrosamine generated decomposing rapidly to give a methyldiazonium ion and formaldehyde. In experiments with bacteriophage suspended in phosphate buffer the biological inactivation observed was the sum of the effects of the formaldehyde and of alkylation by the methylcarbonium ion produced from the diazonium ion. In experiments with bacteriophage suspended in Tris--HCl buffer the effects of formaldehyde were eliminated by its reaction with the buffer component. Alkylation by the carbonium ion produced unstable phosphotriesters in the bacteriophage RNA which on hydrolysis led to degradation of the molecule. In phosphate buffer the formaldehyde cross-linked the protein coat of the bacteriophage blocking the extraction of the RNA. Estimates of the mean lethal dose and of the extent of degradation of the RNA following reaction in Tris--HCl buffer were fairly close to those observed in experiments with N-methyl-N-nitrosourea (MNUA).", "contents": "The interaction of acetoxy-dimethylnitrosamine, a proximate metabolite of the carcinogenic amine, and bacteriophages R17 and T7. The biological and physicochemical effects of reacting bacteriophages R17 and T7 with acetoxy-dimethylnitrosamine (ADMN) have been studied. The rate-determining step in the reactions appeared to be the loss of the acetoxy group by hydrolysis, the hydroxymethyl-methylnitrosamine generated decomposing rapidly to give a methyldiazonium ion and formaldehyde. In experiments with bacteriophage suspended in phosphate buffer the biological inactivation observed was the sum of the effects of the formaldehyde and of alkylation by the methylcarbonium ion produced from the diazonium ion. In experiments with bacteriophage suspended in Tris--HCl buffer the effects of formaldehyde were eliminated by its reaction with the buffer component. Alkylation by the carbonium ion produced unstable phosphotriesters in the bacteriophage RNA which on hydrolysis led to degradation of the molecule. In phosphate buffer the formaldehyde cross-linked the protein coat of the bacteriophage blocking the extraction of the RNA. Estimates of the mean lethal dose and of the extent of degradation of the RNA following reaction in Tris--HCl buffer were fairly close to those observed in experiments with N-methyl-N-nitrosourea (MNUA).", "PMID": 954130}
{"id": "pubmed23n0032_920", "title": "Effects of methylation  of the beta-galactosidase genome upon in vitro synthesis of beta-galactosidase.", "content": "A template DNA from phage lambdah80dlacp5 coding for the in vitro synthesis of beta-galactosidase was used to study the effect of DNA methylation by the alkylating agent, dimethyl sulfate (DMS). Increasing the levels of DMS up to 50 mM concentration in the incubation medium led to an increase of DNA methylation. When incubated for 10 min at 37 degrees C, 3-4% Of nucleotides were methylated. The increase was linear to about 0.6% nucleotide methylation level. A higher yield was obtained at 37 degrees C incubation temperature than at 20 degrees C. Methylation of lambdah80dlacp5 DNA alone without methylation of other factors in the incubation mixture caused inhibition of the synthesis of beta-galactosidase in vitro. Increasing levels of DNA methylation caused greater inhibition of the newly synthesized enzyme activity. Total protein and RNA synthesis was inhibited by the methylated DNA to a much lesser extent than the inhibition of enzyme activity. When the level of nucleotide methylation was 0.74%, only 2% of enzyme activity remained, but total protein and RNA synthetic activities were found to be 72% and 44%, respectively.", "contents": "Effects of methylation  of the beta-galactosidase genome upon in vitro synthesis of beta-galactosidase. A template DNA from phage lambdah80dlacp5 coding for the in vitro synthesis of beta-galactosidase was used to study the effect of DNA methylation by the alkylating agent, dimethyl sulfate (DMS). Increasing the levels of DMS up to 50 mM concentration in the incubation medium led to an increase of DNA methylation. When incubated for 10 min at 37 degrees C, 3-4% Of nucleotides were methylated. The increase was linear to about 0.6% nucleotide methylation level. A higher yield was obtained at 37 degrees C incubation temperature than at 20 degrees C. Methylation of lambdah80dlacp5 DNA alone without methylation of other factors in the incubation mixture caused inhibition of the synthesis of beta-galactosidase in vitro. Increasing levels of DNA methylation caused greater inhibition of the newly synthesized enzyme activity. Total protein and RNA synthesis was inhibited by the methylated DNA to a much lesser extent than the inhibition of enzyme activity. When the level of nucleotide methylation was 0.74%, only 2% of enzyme activity remained, but total protein and RNA synthetic activities were found to be 72% and 44%, respectively.", "PMID": 954131}
{"id": "pubmed23n0032_921", "title": "The 7-methylbenz(a)anthracene deoxyribonucleoside products isolated from DNA after metabolism of the carcinogen by rat liver microsomes in the presence of DNA.", "content": "Metabolism of 7-methylbenz[a]anthracene (7MeBA) by 3-methylcholanthrene-induced rat liver microsomes in the presence of added native or denatured DNA resulted in covalent binding of the hydrocarbon to the nucleic acid. Enzymatic degradation and column chromatographic fractionation showed that the hydrocarbon-deoxyribonucleoside products were separable from the products similarly obtained from DNA having 7MeBA bound following treatment of mouse embryo cells in culture with this hydrocarbon. Comparison of the microsome catalysed hydrocarbon-deoxyribonucleoside products with those obtained by reaction with DNA of 7MeBA-5,6-oxide suggested that this K-region epoxide made a significant contribution to the liver microsome-induced DNA binding.", "contents": "The 7-methylbenz(a)anthracene deoxyribonucleoside products isolated from DNA after metabolism of the carcinogen by rat liver microsomes in the presence of DNA. Metabolism of 7-methylbenz[a]anthracene (7MeBA) by 3-methylcholanthrene-induced rat liver microsomes in the presence of added native or denatured DNA resulted in covalent binding of the hydrocarbon to the nucleic acid. Enzymatic degradation and column chromatographic fractionation showed that the hydrocarbon-deoxyribonucleoside products were separable from the products similarly obtained from DNA having 7MeBA bound following treatment of mouse embryo cells in culture with this hydrocarbon. Comparison of the microsome catalysed hydrocarbon-deoxyribonucleoside products with those obtained by reaction with DNA of 7MeBA-5,6-oxide suggested that this K-region epoxide made a significant contribution to the liver microsome-induced DNA binding.", "PMID": 954132}
{"id": "pubmed23n0032_922", "title": "PHA response and methylazoxymethanol acetate.", "content": "Several reports have indicated the mutagenicity of the cycasin aglycone, methyl azoxymethanol (MAM). Van Den Berg and Ball (1972) have demonstrated the inhibitory effects of MAM acetate (MAMac), a more stable form of the aglycone, on DNA synthesis and cell proliferation in HeLa cells. The purpose of this study was to observe the effects of MAMac on blastogenesis in the human short-term leucocyte culture system. A depression in blastogenesis by MAMac was observed cytologically derived from two individuals. The same effect was observed utilizing [3H] thymidine as an indicator of blastogenesis in a series of cultures from 11 male individuals exposed to varying doses of MAMac, ranging from 5 through 800 mug/ml.", "contents": "PHA response and methylazoxymethanol acetate. Several reports have indicated the mutagenicity of the cycasin aglycone, methyl azoxymethanol (MAM). Van Den Berg and Ball (1972) have demonstrated the inhibitory effects of MAM acetate (MAMac), a more stable form of the aglycone, on DNA synthesis and cell proliferation in HeLa cells. The purpose of this study was to observe the effects of MAMac on blastogenesis in the human short-term leucocyte culture system. A depression in blastogenesis by MAMac was observed cytologically derived from two individuals. The same effect was observed utilizing [3H] thymidine as an indicator of blastogenesis in a series of cultures from 11 male individuals exposed to varying doses of MAMac, ranging from 5 through 800 mug/ml.", "PMID": 954133}
{"id": "pubmed23n0032_923", "title": "Metabolic interaction of secondary amines and tertiary amino propiophenones with monoamine oxidase systems.", "content": "The metabolic interaction of three secondary amines and three nitrogen-containing metabolites of safrole (tertiary amino propiophenones) with rat liver mitochondrial monoamine oxidase systems was studied in vitro employing [7-14C] benzylamine--HCl as a substrate. The two cyclic secondary amines, piperidine and pyrrolidine, showed hyperbolic competitive inhibition while pure competitive inhibition was observed in case of dimethylamine--HCl and three safrole metabolites. Inhibition characteristics for rat liver, kidney and brain mitochondrial monoamine oxidase with two cyclic secondary amines and tertiary amino with two cyclic secondary amines and tertiary amino propiophenones of safrole and elemicin were investigated manometrically using tyramine and serotonin as the substrates.", "contents": "Metabolic interaction of secondary amines and tertiary amino propiophenones with monoamine oxidase systems. The metabolic interaction of three secondary amines and three nitrogen-containing metabolites of safrole (tertiary amino propiophenones) with rat liver mitochondrial monoamine oxidase systems was studied in vitro employing [7-14C] benzylamine--HCl as a substrate. The two cyclic secondary amines, piperidine and pyrrolidine, showed hyperbolic competitive inhibition while pure competitive inhibition was observed in case of dimethylamine--HCl and three safrole metabolites. Inhibition characteristics for rat liver, kidney and brain mitochondrial monoamine oxidase with two cyclic secondary amines and tertiary amino with two cyclic secondary amines and tertiary amino propiophenones of safrole and elemicin were investigated manometrically using tyramine and serotonin as the substrates.", "PMID": 954134}
{"id": "pubmed23n0032_924", "title": "The binding of ethyl carbamate to DNA of mouse liver in vivo: the nature of the bound molecule and the site of binding.", "content": "Ethyl carbamate, labelled at C1 with 14C, bound in vivo to liver DNA of intact and partially hepatectomised mice. Isotope (18O) enrichment was not detected in the oxygen of liver DNA of mice injected with [18O] ethyl carbamate, C2H5--18O--CO--NH2. This suggests that it was the ethyl group and not the ethoxy group which bound to DNA. Chromatographic analysis of acid hydrolysates of liver DNA from mice treated with [1-14C] ethyl carbamate provided no evidence of alkylation or other form of binding to purine or pyrimidine bases. On relatively mild acid hydrolysis the alkyl group remained bound to the \"apurinic acid\" fraction, while more vigorous hydrolysis lead progressively first to its separation as highly ionisable hydrophilic non-volatile compounds and then to its loss as a volatile compound. DNAase I followed by phosphodiesterase hydrolysis also split off the 14C-containing group as a volatile compound. The volatile compound was identified as ethanol. These results suggest that the alkyl group was bound as an ester to a phosphate group in the DNA chain. Results with DNA from partially hepatectomised mice did not differ from those with DNA from intact mice.", "contents": "The binding of ethyl carbamate to DNA of mouse liver in vivo: the nature of the bound molecule and the site of binding. Ethyl carbamate, labelled at C1 with 14C, bound in vivo to liver DNA of intact and partially hepatectomised mice. Isotope (18O) enrichment was not detected in the oxygen of liver DNA of mice injected with [18O] ethyl carbamate, C2H5--18O--CO--NH2. This suggests that it was the ethyl group and not the ethoxy group which bound to DNA. Chromatographic analysis of acid hydrolysates of liver DNA from mice treated with [1-14C] ethyl carbamate provided no evidence of alkylation or other form of binding to purine or pyrimidine bases. On relatively mild acid hydrolysis the alkyl group remained bound to the \"apurinic acid\" fraction, while more vigorous hydrolysis lead progressively first to its separation as highly ionisable hydrophilic non-volatile compounds and then to its loss as a volatile compound. DNAase I followed by phosphodiesterase hydrolysis also split off the 14C-containing group as a volatile compound. The volatile compound was identified as ethanol. These results suggest that the alkyl group was bound as an ester to a phosphate group in the DNA chain. Results with DNA from partially hepatectomised mice did not differ from those with DNA from intact mice.", "PMID": 954135}
{"id": "pubmed23n0032_925", "title": "Biotransformation of 4-dimethylaminophenol: reaction with glutathione, and some properties of the reaction products.", "content": "4-Dimethylaminophenol (DMAP) forms ferrihemoglobin by catalytic transfer of electrons from ferrohemoglobin to oxygen. In solutions of purified human hemoglobin, quick binding of oxidized DMAP to the globin moiety of hemoglobin terminates this reaction. Reduced glutathione in high concentrations, as in the red cell, substantially diminished binding of oxidized DMAP to hemoglobin by formation of S,S,S-(2-dimethylamino-5-hydroxy-1,3,4-phenylene)-tris-glutathione (tris-(GS)-DMAP), which does not form ferrihemoglobin. In the presence of reduced glutathione, DMAP disappeared more rapidly from hemoglobin solutions than in its absence. The formation of tris(GS)-DMAP in red  cells was found to be of importance for the termination of catalytic ferrihemoglobin formation by DMAP in vivo. With low concentrations of GSH, DMAP in hemoglobin solutions formed another conjugate, (GS)-DMAP, S,S(2-dimethylamino-5-hydroxy-1,3-phenylene)-bis-glutathione. Similar to DMAP, bis(GS)-DMAP catalyzed the formation of ferrihemoglobin. As the oxidized bis(GS)-DMAP was bound to hemoglobin more slowly and to a lesser extent, it produced more ferrihemoglobin than DMAP. In contrast to the reactions of DMAP with hemoglobin, hydrogen peroxide and superoxide radicals are involved in the ferrihemoglobin formation by bis(GS)-DMAP. The radicals accelerate the oxidation of bis(GS)-DMAP and thereby the ferrihemoglobin formation.", "contents": "Biotransformation of 4-dimethylaminophenol: reaction with glutathione, and some properties of the reaction products. 4-Dimethylaminophenol (DMAP) forms ferrihemoglobin by catalytic transfer of electrons from ferrohemoglobin to oxygen. In solutions of purified human hemoglobin, quick binding of oxidized DMAP to the globin moiety of hemoglobin terminates this reaction. Reduced glutathione in high concentrations, as in the red cell, substantially diminished binding of oxidized DMAP to hemoglobin by formation of S,S,S-(2-dimethylamino-5-hydroxy-1,3,4-phenylene)-tris-glutathione (tris-(GS)-DMAP), which does not form ferrihemoglobin. In the presence of reduced glutathione, DMAP disappeared more rapidly from hemoglobin solutions than in its absence. The formation of tris(GS)-DMAP in red  cells was found to be of importance for the termination of catalytic ferrihemoglobin formation by DMAP in vivo. With low concentrations of GSH, DMAP in hemoglobin solutions formed another conjugate, (GS)-DMAP, S,S(2-dimethylamino-5-hydroxy-1,3-phenylene)-bis-glutathione. Similar to DMAP, bis(GS)-DMAP catalyzed the formation of ferrihemoglobin. As the oxidized bis(GS)-DMAP was bound to hemoglobin more slowly and to a lesser extent, it produced more ferrihemoglobin than DMAP. In contrast to the reactions of DMAP with hemoglobin, hydrogen peroxide and superoxide radicals are involved in the ferrihemoglobin formation by bis(GS)-DMAP. The radicals accelerate the oxidation of bis(GS)-DMAP and thereby the ferrihemoglobin formation.", "PMID": 954136}
{"id": "pubmed23n0032_926", "title": "Platinum complexes of substituted ethylenediamines and their anti-tumour activity.", "content": "A large number of substituted ethylenediamine complexes of platinum (II) and platinum(IV) have been prepared and characterised, and their anti-tumour activity tested against sarcoma 180, leukemia L1210, and ADJ/PC6A plasma cell tumours. In general these complexes are less active than simpler amine analogues, though several complexes show activity against all of the screens. Difference in activity of platinum(II) and platinum(IV) analogues leads us to cast doubt on the \"in vivo reduction\" theory hitherto used to describe the action  of platinum(IV) complexes.", "contents": "Platinum complexes of substituted ethylenediamines and their anti-tumour activity. A large number of substituted ethylenediamine complexes of platinum (II) and platinum(IV) have been prepared and characterised, and their anti-tumour activity tested against sarcoma 180, leukemia L1210, and ADJ/PC6A plasma cell tumours. In general these complexes are less active than simpler amine analogues, though several complexes show activity against all of the screens. Difference in activity of platinum(II) and platinum(IV) analogues leads us to cast doubt on the \"in vivo reduction\" theory hitherto used to describe the action  of platinum(IV) complexes.", "PMID": 954137}
{"id": "pubmed23n0032_927", "title": "The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of P388F lymphoma cells in culture. I. Alkali dissociable DNA lesions and their restitution.", "content": "The pattern of formation and rejoining of P388F cell DNA single strand breaks revealed by alkaline sedimentation centrifugation studies following treatment with methyl nitro-nitrosoguanidine (MNNG) has been studied. Three periods of single strand break formation have been recognised, an initial period during the first 4 h where breaks are rapidly formed and rejoined, a second (medial) time range of 10-15 h following treatment and a final stage where the formation of breaks appears to be related to cell killing processes. The initial phase is considered to be due to the production and repair of X-ray like DNA lesions whereas those of the medial time range are more comparable to the enzymatic processes associated with the repair of ultraviolet light-induced damage.", "contents": "The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of P388F lymphoma cells in culture. I. Alkali dissociable DNA lesions and their restitution. The pattern of formation and rejoining of P388F cell DNA single strand breaks revealed by alkaline sedimentation centrifugation studies following treatment with methyl nitro-nitrosoguanidine (MNNG) has been studied. Three periods of single strand break formation have been recognised, an initial period during the first 4 h where breaks are rapidly formed and rejoined, a second (medial) time range of 10-15 h following treatment and a final stage where the formation of breaks appears to be related to cell killing processes. The initial phase is considered to be due to the production and repair of X-ray like DNA lesions whereas those of the medial time range are more comparable to the enzymatic processes associated with the repair of ultraviolet light-induced damage.", "PMID": 954139}
{"id": "pubmed23n0032_928", "title": "The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage.", "content": "Repair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed.", "contents": "The time course of DNA repair following methyl nitro-nitrosoguanidine (MNNG) treatment of p388f lymphoma cells in culture. II. DNA repair synthesis and its correlation with strand breakage. Repair synthesis has been followed in P388F cell DNA following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) using 5-iodo-deoxyuridine (IUdR) and [3H] thymidine combination. A pattern of repair synthesis was obtained, similar in its timing to the initial and medial stages of single-strand break formation and rejoining within the first 24 h after treatment. A higher base insertion frequency in relation to single-strand breaks occurs during the medial stage of repair. However, a fundamental problem with the use of iododeoxyuridine and [3H] thymidine ([3H] TdR) as a suitable measure of repair synthesis was encountered and a potential source of error in such experiments was observed.", "PMID": 954140}
{"id": "pubmed23n0032_929", "title": "Effects of actinomycin D and puromycin on colchicine-induced endoreduplication in Chinese hamster cells in vitro.", "content": "Experiments were carried out to study the events through which the process of endoreduplication occurs after treatment with colchicine in Chinese hamster cells cultivated in vitro, and to analyse the differences between the processes leading to normal mitosis and those leading to endoreduplication. Chinese hamster cells (line C-125) were treated with colchicine (10(-4) M) for 1 h, in order to induce endoreduplication. Before and after colchicine treatment the cultures were treated at various intervals with actinomycin D and puromycin. For preparation of mitosis specific RNA synthesis is required, and also synthesis of a specific protein, which is not required for endoreduplication. Moreover this suggests not only that the cells that endoreduplicate do not need new RNA and protein synthesis, but that inhibition of their synthesis apparently favours the processes of endoreduplication.", "contents": "Effects of actinomycin D and puromycin on colchicine-induced endoreduplication in Chinese hamster cells in vitro. Experiments were carried out to study the events through which the process of endoreduplication occurs after treatment with colchicine in Chinese hamster cells cultivated in vitro, and to analyse the differences between the processes leading to normal mitosis and those leading to endoreduplication. Chinese hamster cells (line C-125) were treated with colchicine (10(-4) M) for 1 h, in order to induce endoreduplication. Before and after colchicine treatment the cultures were treated at various intervals with actinomycin D and puromycin. For preparation of mitosis specific RNA synthesis is required, and also synthesis of a specific protein, which is not required for endoreduplication. Moreover this suggests not only that the cells that endoreduplicate do not need new RNA and protein synthesis, but that inhibition of their synthesis apparently favours the processes of endoreduplication.", "PMID": 954141}
{"id": "pubmed23n0032_930", "title": "Actinomycin D-induced enhancement of ubiquinone biosynthesis in rat.", "content": "Administration of actinomycin D to fasted rats induces an enhancement of the labeling of hepatic ubiquinone by [2-14C] acetate both in vivo and in vitro. The incorporation of [2-14C] mevalonate into ubiquinone is also increased, although to a significantly lesser extent; this, however, presumably results from greater uptake of the labeled precursor by liver of drug-treated rats. The drug-administered animals show increased activity of liver microsomal mevalonate: NADP oxidoreductase, the rate-limiting enzyme in isoprenoid biogenesis. The incorporation of [u-14C] benzoic acid and CH3-[14C] methionine into ubiquinone in liver slices, however, reveals a decrease in actinomycin D administered rats. This appears to be due to a specific inhibition of the pathway leading to the benzoquinone moiety of ubiquinone and not to an increase in the pool-size of the precursors. The stimulatory effect of the drug on ubiquinone biosynthesis is also observable in cholesterol-fed rats. The actinomycin D-induced increase in ubiquinone biosynthesis is dependent on new protein synthesis since the effect is abolished by treating the animals with either cycloheximide or puromycin.", "contents": "Actinomycin D-induced enhancement of ubiquinone biosynthesis in rat. Administration of actinomycin D to fasted rats induces an enhancement of the labeling of hepatic ubiquinone by [2-14C] acetate both in vivo and in vitro. The incorporation of [2-14C] mevalonate into ubiquinone is also increased, although to a significantly lesser extent; this, however, presumably results from greater uptake of the labeled precursor by liver of drug-treated rats. The drug-administered animals show increased activity of liver microsomal mevalonate: NADP oxidoreductase, the rate-limiting enzyme in isoprenoid biogenesis. The incorporation of [u-14C] benzoic acid and CH3-[14C] methionine into ubiquinone in liver slices, however, reveals a decrease in actinomycin D administered rats. This appears to be due to a specific inhibition of the pathway leading to the benzoquinone moiety of ubiquinone and not to an increase in the pool-size of the precursors. The stimulatory effect of the drug on ubiquinone biosynthesis is also observable in cholesterol-fed rats. The actinomycin D-induced increase in ubiquinone biosynthesis is dependent on new protein synthesis since the effect is abolished by treating the animals with either cycloheximide or puromycin.", "PMID": 954142}
{"id": "pubmed23n0032_931", "title": "Action of a platinum complex [cis-dichlorobis (cyclopentylamine)-platinum (II)] on Chinese hamster ovary cells in vitro.", "content": "cis-Dichlorobis(cyclopentylamine)platinum (II) (DBCP) treatment of Chinese hamster ovary (CHO) monolayer cell cultures was found to cause: (1) growth inhibition due to an impaired G1 leads to S transition and formation of a non-cycling compartment; (2) delayed cell death (with a maximum at 72 h after treatment); (3) decrease of [14C] TdR incorporation into DNA. Part of the surviving cell population was subject to non-lethal damage. The Do values of the dose--survival curves were 9.2 and 14 mug/ml in two kinds of media differing in Na+ and Cl- concentrations. There was no sparing effect of dose fractionation and no cell cycle phase specificity. In the dose range corresponding to the shoulder region of the dose-survival curve,the lethal effect of DBCP treatment was directly related to the decreased rate of DNA synthesis in DBCP-treated cells.", "contents": "Action of a platinum complex [cis-dichlorobis (cyclopentylamine)-platinum (II)] on Chinese hamster ovary cells in vitro. cis-Dichlorobis(cyclopentylamine)platinum (II) (DBCP) treatment of Chinese hamster ovary (CHO) monolayer cell cultures was found to cause: (1) growth inhibition due to an impaired G1 leads to S transition and formation of a non-cycling compartment; (2) delayed cell death (with a maximum at 72 h after treatment); (3) decrease of [14C] TdR incorporation into DNA. Part of the surviving cell population was subject to non-lethal damage. The Do values of the dose--survival curves were 9.2 and 14 mug/ml in two kinds of media differing in Na+ and Cl- concentrations. There was no sparing effect of dose fractionation and no cell cycle phase specificity. In the dose range corresponding to the shoulder region of the dose-survival curve,the lethal effect of DBCP treatment was directly related to the decreased rate of DNA synthesis in DBCP-treated cells.", "PMID": 954143}
{"id": "pubmed23n0032_932", "title": "Inhibition of viral multiplication by homologous methylated ribonucleic acids. V. Inhibition of myxoviruses in mouse.", "content": "Mice respond to intravenous injection of homologous methylated RNA by the production of a circulating interferon-like substance. The treatment with modified RNA induces a significant protection against viral infection. This effect is optimum when the treatment occurs a few hours before viral infection. The protective effect is a peculiar property of homologous methylated RNA as heterologous RNAs do not induce any resistance to the infection with influenza virus.", "contents": "Inhibition of viral multiplication by homologous methylated ribonucleic acids. V. Inhibition of myxoviruses in mouse. Mice respond to intravenous injection of homologous methylated RNA by the production of a circulating interferon-like substance. The treatment with modified RNA induces a significant protection against viral infection. This effect is optimum when the treatment occurs a few hours before viral infection. The protective effect is a peculiar property of homologous methylated RNA as heterologous RNAs do not induce any resistance to the infection with influenza virus.", "PMID": 954144}
{"id": "pubmed23n0032_933", "title": "Distribution and cytogenetic test of 2,4-D and 2,4,5-T phenoxyacetic acids in mouse blood tissues.", "content": "The phenoxyacetic acids 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), extensively used as herbicides, were tested for cytogenetic effects by means of induced micronuclei in erythrocytes of mouse bone marrow.  Because of the hig experimental resolution power this is a particularly suitable test system for the detection of weak chromosome breaking activity in mammals.  The cytogenetic tests were supplemented with chemical analyses of the concentration the the test substances reaching the target cells...", "contents": "Distribution and cytogenetic test of 2,4-D and 2,4,5-T phenoxyacetic acids in mouse blood tissues. The phenoxyacetic acids 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), extensively used as herbicides, were tested for cytogenetic effects by means of induced micronuclei in erythrocytes of mouse bone marrow.  Because of the hig experimental resolution power this is a particularly suitable test system for the detection of weak chromosome breaking activity in mammals.  The cytogenetic tests were supplemented with chemical analyses of the concentration the the test substances reaching the target cells...", "PMID": 954145}
{"id": "pubmed23n0032_934", "title": "The effect of pre-treatment with phenobarbitone on the activation of aflatoxin B1 by rat liver.", "content": "Microsomes prepared from rats pre-treated with phenobarbitone are more effective in activating aflatoxin B1 in vitro to a metabolite which inhibits RNA polymerase than are microsomes obtained from control animals. This result is in contrast with the situation in vivo where pre-treatment with phenobarbitone protects against inhibition of RNA synthesis by aflatoxin B1. The hypothesis is advanced that, in vivo, the activation of aflatoxin B1 which is significant in terms of inhibition of nucleic acid synthesis largely occurs on the outer nuclear membrane, and that by increasing activation by the microsomes, phenobarbitone pre-treatment reduces the amount of aflatoxin B1 available for the nuclear activation.", "contents": "The effect of pre-treatment with phenobarbitone on the activation of aflatoxin B1 by rat liver. Microsomes prepared from rats pre-treated with phenobarbitone are more effective in activating aflatoxin B1 in vitro to a metabolite which inhibits RNA polymerase than are microsomes obtained from control animals. This result is in contrast with the situation in vivo where pre-treatment with phenobarbitone protects against inhibition of RNA synthesis by aflatoxin B1. The hypothesis is advanced that, in vivo, the activation of aflatoxin B1 which is significant in terms of inhibition of nucleic acid synthesis largely occurs on the outer nuclear membrane, and that by increasing activation by the microsomes, phenobarbitone pre-treatment reduces the amount of aflatoxin B1 available for the nuclear activation.", "PMID": 954146}
{"id": "pubmed23n0032_935", "title": "Urinary metabolites of 3,3-dimethyl-1-phenyltriazene.", "content": "Urinary metabolites excreted after a subcutaneous injection of 3,3-dimethyl-1[14C] phenyltriazene (DM[1-14C]PT) to rats accounted for 82% of the applied radioactivity. We have isolated aniline (1-2%), 2-hydroxyaniline (5-7%), 3-hydroxyaniline (about 1%) and 4-hydroxyaniline (31-37%) from ethyl acetate extracts of acid-hydrolysed urine, UV spectrometric determination of 4-hydroxyaniline, using the indophenol reaction, showed that the most abundant metabolite accounted for 56 to 61% of the applied dose. We have also demonstrated the excretion of metabolites containing the intact triazene structure (0.9-1.1%) by cold acid cleavage of these compounds, followed by coupling of the released arenediazonium cations with N-ethyl-1-naphthylamine (EN). The coloured derivatives of these metabolites, 4-benzeneazo-N-ethyl-1-naphthylamine (BAEN) (0.6-0.7%), 4-(2-hydroxybenzeneazo)-N-ethyl-1-napthylamine (2-HO-BAEN) (0.02%) and 4-(4-hydroxybenzeneazo)-N-ethyl-1-naphthylamine (4-HO-BAEN) (0.3-0.4%) were isolated. The identification of BAEN as the principal azo derivative of the excreted triazene metabolites is in full agreement with the proposed in vivo activation of 3,3-dimethyl-1-phenyltriazene (DMPT) to a carcinogenic methylating agent. The hydroxylation of the methyl group at N-3 yields the corresponding aminol, some of which is covalently bonded to a water-soluble compound.", "contents": "Urinary metabolites of 3,3-dimethyl-1-phenyltriazene. Urinary metabolites excreted after a subcutaneous injection of 3,3-dimethyl-1[14C] phenyltriazene (DM[1-14C]PT) to rats accounted for 82% of the applied radioactivity. We have isolated aniline (1-2%), 2-hydroxyaniline (5-7%), 3-hydroxyaniline (about 1%) and 4-hydroxyaniline (31-37%) from ethyl acetate extracts of acid-hydrolysed urine, UV spectrometric determination of 4-hydroxyaniline, using the indophenol reaction, showed that the most abundant metabolite accounted for 56 to 61% of the applied dose. We have also demonstrated the excretion of metabolites containing the intact triazene structure (0.9-1.1%) by cold acid cleavage of these compounds, followed by coupling of the released arenediazonium cations with N-ethyl-1-naphthylamine (EN). The coloured derivatives of these metabolites, 4-benzeneazo-N-ethyl-1-naphthylamine (BAEN) (0.6-0.7%), 4-(2-hydroxybenzeneazo)-N-ethyl-1-napthylamine (2-HO-BAEN) (0.02%) and 4-(4-hydroxybenzeneazo)-N-ethyl-1-naphthylamine (4-HO-BAEN) (0.3-0.4%) were isolated. The identification of BAEN as the principal azo derivative of the excreted triazene metabolites is in full agreement with the proposed in vivo activation of 3,3-dimethyl-1-phenyltriazene (DMPT) to a carcinogenic methylating agent. The hydroxylation of the methyl group at N-3 yields the corresponding aminol, some of which is covalently bonded to a water-soluble compound.", "PMID": 954147}
{"id": "pubmed23n0032_936", "title": "The effect of oral doses of a polythiol resin on the excretion of methylmercury in mice treated with cysteine, D-penicillamine or phenobarbitone.", "content": "Mice dosed with methylmercury chloride (0.5 to 2.0 mg Hg/kg i.p.) labeled with the 203Hg isotope were given polythiol resin in their food (2% w/w). The effect of pretreatment with phenobarbitone, or co-administration of D-penicillamine or L-cysteine, was tested on the efficacy of the resin to accelerate the excretion of methylmercury. The effect of the polythiol resin in enhancing fecal excretion was dependent on the dose of methylmercury, it being significantly greater at doses of 1.0 and 2.0 than at 0.5 mg Hg/kg. In animals given the resin and phenobarbitone (0.1% w/w drinking water), fecal excretion was approx. 36% greater than in animals given resin alone. Penicillamine (2.5 mg/ml drinking water) actually retarted elimination of methylmercury in animals given the resin. Despite the enhanced efficacy of the resin plus phenobarbitone, the brain levels of methylmercury were not reduced beyond those seen in animals given resin alone. The mechanisms and implications of these effects are discussed.", "contents": "The effect of oral doses of a polythiol resin on the excretion of methylmercury in mice treated with cysteine, D-penicillamine or phenobarbitone. Mice dosed with methylmercury chloride (0.5 to 2.0 mg Hg/kg i.p.) labeled with the 203Hg isotope were given polythiol resin in their food (2% w/w). The effect of pretreatment with phenobarbitone, or co-administration of D-penicillamine or L-cysteine, was tested on the efficacy of the resin to accelerate the excretion of methylmercury. The effect of the polythiol resin in enhancing fecal excretion was dependent on the dose of methylmercury, it being significantly greater at doses of 1.0 and 2.0 than at 0.5 mg Hg/kg. In animals given the resin and phenobarbitone (0.1% w/w drinking water), fecal excretion was approx. 36% greater than in animals given resin alone. Penicillamine (2.5 mg/ml drinking water) actually retarted elimination of methylmercury in animals given the resin. Despite the enhanced efficacy of the resin plus phenobarbitone, the brain levels of methylmercury were not reduced beyond those seen in animals given resin alone. The mechanisms and implications of these effects are discussed.", "PMID": 954148}
{"id": "pubmed23n0032_937", "title": "A new class of inhibitory cytochrome P-450 complexes formed during metabolism: a comparison with amphetamine and SKF 525-A type complexes.", "content": "During the metabolism of p-chloroaniline, sulfanilamide, 2-methylindoline, and dapsone, complexes with cytochrome P-450 are formed which absorb maximally at 448, 450, 451, and 452 nm, respectively. These complexes are similar to the complexes from amphetamines (456 nm) and SKF 525-A (452 nm) in the conditions for, and rate of their formation, and their inhibition of ethylmorphine N-demethylase activity. Similarly, the formation is mainly a property of phenobarbital-induced cytochrome P-450 and is inhibited by comparable concentrations of metyrapone and 2-O-iodophenoxymethylimidazole. Unlike the complexes from amphetamine and SKF 525-A, those formed from p-chloroaniline, sulfanilamide, 2-methylindoline, and dapsone are unstable in the presence of sodium dithionite.", "contents": "A new class of inhibitory cytochrome P-450 complexes formed during metabolism: a comparison with amphetamine and SKF 525-A type complexes. During the metabolism of p-chloroaniline, sulfanilamide, 2-methylindoline, and dapsone, complexes with cytochrome P-450 are formed which absorb maximally at 448, 450, 451, and 452 nm, respectively. These complexes are similar to the complexes from amphetamines (456 nm) and SKF 525-A (452 nm) in the conditions for, and rate of their formation, and their inhibition of ethylmorphine N-demethylase activity. Similarly, the formation is mainly a property of phenobarbital-induced cytochrome P-450 and is inhibited by comparable concentrations of metyrapone and 2-O-iodophenoxymethylimidazole. Unlike the complexes from amphetamine and SKF 525-A, those formed from p-chloroaniline, sulfanilamide, 2-methylindoline, and dapsone are unstable in the presence of sodium dithionite.", "PMID": 954149}
{"id": "pubmed23n0032_938", "title": "Cadmium-thionein and the protection by cadmium against the nephrotoxicity of mercury.", "content": "Uptake of Hg2+ into the renal and hepatic metallothioneins of rats is increased by pretreatment with Cd2+. This increased uptake occurs both by displacement of Cd2+ (and of Zn2+) from the presynthesized cadmium-thionein, and by further synthesis of thionein. The former mechanism predominates in the kidney of the male rat, which is more sensitive than the female to Hg2+. The latter mechanism, which occurs particularly in the kidney of the female, also is considered to involve an initial displacement of Cd2+ from cadmium-thionein, but is followed by further synthesis of the metalloprotein, which is induced by the liberated cation. Pretreatment with Cd2+ increases not only the incorporation of Hg2+ into the renal metallothionein, but also the uptake of Hg2+ into other components of the kidney. At dose levels of Hg2+ at which Cd2+-pretreatment gives complete protection against the nephrotoxicity in male and female rats, the increase in Hg2+-uptake into both the particulate components and into the soluble fraction of the kidney is greater than into metallothionein. It is concluded, therefore, that binding of Hg2+ by pre-induced cadmium-thionein alone cannot explain the protection by Cd2+ against the nephrotoxicity of Hg2+.", "contents": "Cadmium-thionein and the protection by cadmium against the nephrotoxicity of mercury. Uptake of Hg2+ into the renal and hepatic metallothioneins of rats is increased by pretreatment with Cd2+. This increased uptake occurs both by displacement of Cd2+ (and of Zn2+) from the presynthesized cadmium-thionein, and by further synthesis of thionein. The former mechanism predominates in the kidney of the male rat, which is more sensitive than the female to Hg2+. The latter mechanism, which occurs particularly in the kidney of the female, also is considered to involve an initial displacement of Cd2+ from cadmium-thionein, but is followed by further synthesis of the metalloprotein, which is induced by the liberated cation. Pretreatment with Cd2+ increases not only the incorporation of Hg2+ into the renal metallothionein, but also the uptake of Hg2+ into other components of the kidney. At dose levels of Hg2+ at which Cd2+-pretreatment gives complete protection against the nephrotoxicity in male and female rats, the increase in Hg2+-uptake into both the particulate components and into the soluble fraction of the kidney is greater than into metallothionein. It is concluded, therefore, that binding of Hg2+ by pre-induced cadmium-thionein alone cannot explain the protection by Cd2+ against the nephrotoxicity of Hg2+.", "PMID": 954150}
{"id": "pubmed23n0032_939", "title": "\"Paired reading\" tuition: a preliminary report on a technique for cases of reading deficit.", "content": "A simple and flexible reading tuition procedure is described, incorporating simultaneous reading and verbally reinforced individual reading, utilizing any reading material of the child's choice, and maximizing continuous adaptation to individual reading behaviour. Three reading-deficient children tutored by \"paired reading\" are presented, indicating a clear increase in the percentage of words correctly read and reaching statistical significance over twelve hours of tuition.", "contents": "\"Paired reading\" tuition: a preliminary report on a technique for cases of reading deficit. A simple and flexible reading tuition procedure is described, incorporating simultaneous reading and verbally reinforced individual reading, utilizing any reading material of the child's choice, and maximizing continuous adaptation to individual reading behaviour. Three reading-deficient children tutored by \"paired reading\" are presented, indicating a clear increase in the percentage of words correctly read and reaching statistical significance over twelve hours of tuition.", "PMID": 954155}
{"id": "pubmed23n0032_940", "title": "The effect of hypoxia on the regional distribution of cardiac output in the dog.", "content": "Twenty-one dogs were studied under conditions of normal oxygenation and hypoxia with the microsphere distribution method to determine the effect of arterial oxygen saturation on the regional distribution of cardiac output. The dogs were anesthetized and artifically ventilated. Cannulas were placed in the left ventricle to administer microspheres and in a peripheral artery to determine cardiac output. Each dog received two microsphere injections: (1) while normally oxygenated (room air), and (2) under hypoxia (10% oxygen-90% nitrogen in 10 dogs and 5% oxygen-95% nitrogen in 11 dogs). Absolute cardiac output increased from 87 +/- 15 ml/min per kg to 101 +/- 14 ml/min per kg during mild hypoxia (10% oxygen) (P less than 0.05), and from 73 +/- 17 ml/min per kg to 120 +/- 24 ml/min per kg during severe hypoxia (5% oxygen) (P less than 0.01). Absolute blood flows increased to all organs except skin and muscle during hypoxia, although there were decreases in the fractional distribution of cardiac output to the splanchnic bed and kidney. Striking changes were found in coronary, hepatic, and cerebral circulation, and the organ with, greatest response to hypoxia was the heart, with increased coronary flow of 37% and 285% during exposure to 10% and 5% oxygen, respectively. Hence, low oxygen levels in blood cause redistribution of cardiac output and arterial content plays an important role in blood flow regulation.", "contents": "The effect of hypoxia on the regional distribution of cardiac output in the dog. Twenty-one dogs were studied under conditions of normal oxygenation and hypoxia with the microsphere distribution method to determine the effect of arterial oxygen saturation on the regional distribution of cardiac output. The dogs were anesthetized and artifically ventilated. Cannulas were placed in the left ventricle to administer microspheres and in a peripheral artery to determine cardiac output. Each dog received two microsphere injections: (1) while normally oxygenated (room air), and (2) under hypoxia (10% oxygen-90% nitrogen in 10 dogs and 5% oxygen-95% nitrogen in 11 dogs). Absolute cardiac output increased from 87 +/- 15 ml/min per kg to 101 +/- 14 ml/min per kg during mild hypoxia (10% oxygen) (P less than 0.05), and from 73 +/- 17 ml/min per kg to 120 +/- 24 ml/min per kg during severe hypoxia (5% oxygen) (P less than 0.01). Absolute blood flows increased to all organs except skin and muscle during hypoxia, although there were decreases in the fractional distribution of cardiac output to the splanchnic bed and kidney. Striking changes were found in coronary, hepatic, and cerebral circulation, and the organ with, greatest response to hypoxia was the heart, with increased coronary flow of 37% and 285% during exposure to 10% and 5% oxygen, respectively. Hence, low oxygen levels in blood cause redistribution of cardiac output and arterial content plays an important role in blood flow regulation.", "PMID": 954160}
{"id": "pubmed23n0032_941", "title": "Action potential changes under varied [Na+]0 and [Ca2+]0 indicating the existence of two inward currents in cells of the rabbit atrioventricular node.", "content": "In the perfused rabbit heart,the upstroke of the transmembrane action potential of fibers of the atrioventricular (AV) node presents two distinct components. The first depends strongly on extracellular sdoium concentration, but the degree to which it is activated is influenced by extracellular calcium, as indicated by the correlation between its Vmax and [Ca2+]0. The second component depends on calcium and sodium concentrations and is blocked by Mn ions. An analysis comparing action potentials from atrial (A), atrionodal (AN), and nodal (N) fibers shows that the second component of the upstroke of the action potential contributes 12%, 27%, and 34% to the total depolarization. The results suggest that the upstroke of the nodal action potential results from the activation of two inward currents, as in ordinary cardiac fibers. We postulate that (1) the degree of steady state inactivation of gNa is larger in N than in A fibers because of the low resting potential of the former, and (2) the contribution of the second channel to the upstroke depends on the time course of the previous depolarization and the potential level at which this component is activated.", "contents": "Action potential changes under varied [Na+]0 and [Ca2+]0 indicating the existence of two inward currents in cells of the rabbit atrioventricular node. In the perfused rabbit heart,the upstroke of the transmembrane action potential of fibers of the atrioventricular (AV) node presents two distinct components. The first depends strongly on extracellular sdoium concentration, but the degree to which it is activated is influenced by extracellular calcium, as indicated by the correlation between its Vmax and [Ca2+]0. The second component depends on calcium and sodium concentrations and is blocked by Mn ions. An analysis comparing action potentials from atrial (A), atrionodal (AN), and nodal (N) fibers shows that the second component of the upstroke of the action potential contributes 12%, 27%, and 34% to the total depolarization. The results suggest that the upstroke of the nodal action potential results from the activation of two inward currents, as in ordinary cardiac fibers. We postulate that (1) the degree of steady state inactivation of gNa is larger in N than in A fibers because of the low resting potential of the former, and (2) the contribution of the second channel to the upstroke depends on the time course of the previous depolarization and the potential level at which this component is activated.", "PMID": 954161}
{"id": "pubmed23n0032_942", "title": "Low renal papillary plasma flow in both Dahl and Kyoto rats with spontaneous hypertension.", "content": "Abnormally low plasma flow to renal papilla characterizes Dahl hypertension. When eating a normal Na diet (0.3% NaCl) both hypertension-sensitive (S) rats and hypertension-resistant (R) rats, 16 weeks old, have fairly normal blood pressure (BP), averaging 144 and 129 mm Hg, respectively. However, even in this barely hypertensive state, 18 S rats had a 31% lower papillary plasma flow (Lilienfield method) than 22 R rats, 19.2 ml/100 g of papilla per min compared to 25.6 (P less than 0.001). When a high (8%) NaCl diet was fed for 7 days, R rats increased papillary plasma flow from 25.6 on 0.3% NaCl to 33.8 on 8% NaCl, a 32% rise (P less than 0.001). S rats increased papillary flow from 20.4 to 24.8, a 22% rise (P less than 0.05). When a high (8%) NaCl diet was fed for 4 weeks, R rats increased papillary plasma flow from 25.7 ml/100 g per min on 0.3% NaCl to 29.5 ml/100 g per min on 8% NaCl, a 15% rise (P less than 0.025). S rats increased papillary flow from 17.7 to 20.0 ml/100 g per min (not significant). S rats on 8% NaCl had a papillary flow 32% lower than R rats on 8% NaCl (P less than 0.001). BP of S rats rose to 162 mm Hg after 4 weeks on 8% NaCl; in R rats, BP did not rise at all. S rats on 0.3% NaCl have a low papillary flow even in a borderline hypertensive state. When challenged with 8% NaCl, R rats increased papillary flow, an adaptation possibly important for the natriuresis. S rats failed to achieve this same high papillary flow. Lacking this adaptation, hypertension may then conceivably occur in S rats to accomplish natriuresis through a \"pressure natriuresis\" mechanism. Papillary flow also decreased by 11% in 26 Kyoto 17-week-old spontaneously hypertensives (BP, 182 mm Hg) compared to 24 Kyoto normotensives (BP, 118 mm Hg), 29.5 vs 33.2 ml/100 g per min (P less than 0.001). Thus, low papillary flow exists in both hypertensions.", "contents": "Low renal papillary plasma flow in both Dahl and Kyoto rats with spontaneous hypertension. Abnormally low plasma flow to renal papilla characterizes Dahl hypertension. When eating a normal Na diet (0.3% NaCl) both hypertension-sensitive (S) rats and hypertension-resistant (R) rats, 16 weeks old, have fairly normal blood pressure (BP), averaging 144 and 129 mm Hg, respectively. However, even in this barely hypertensive state, 18 S rats had a 31% lower papillary plasma flow (Lilienfield method) than 22 R rats, 19.2 ml/100 g of papilla per min compared to 25.6 (P less than 0.001). When a high (8%) NaCl diet was fed for 7 days, R rats increased papillary plasma flow from 25.6 on 0.3% NaCl to 33.8 on 8% NaCl, a 32% rise (P less than 0.001). S rats increased papillary flow from 20.4 to 24.8, a 22% rise (P less than 0.05). When a high (8%) NaCl diet was fed for 4 weeks, R rats increased papillary plasma flow from 25.7 ml/100 g per min on 0.3% NaCl to 29.5 ml/100 g per min on 8% NaCl, a 15% rise (P less than 0.025). S rats increased papillary flow from 17.7 to 20.0 ml/100 g per min (not significant). S rats on 8% NaCl had a papillary flow 32% lower than R rats on 8% NaCl (P less than 0.001). BP of S rats rose to 162 mm Hg after 4 weeks on 8% NaCl; in R rats, BP did not rise at all. S rats on 0.3% NaCl have a low papillary flow even in a borderline hypertensive state. When challenged with 8% NaCl, R rats increased papillary flow, an adaptation possibly important for the natriuresis. S rats failed to achieve this same high papillary flow. Lacking this adaptation, hypertension may then conceivably occur in S rats to accomplish natriuresis through a \"pressure natriuresis\" mechanism. Papillary flow also decreased by 11% in 26 Kyoto 17-week-old spontaneously hypertensives (BP, 182 mm Hg) compared to 24 Kyoto normotensives (BP, 118 mm Hg), 29.5 vs 33.2 ml/100 g per min (P less than 0.001). Thus, low papillary flow exists in both hypertensions.", "PMID": 954162}
{"id": "pubmed23n0032_943", "title": "Follow-up ?at II years of 46 children with severe unilateral hearing loss at 7 years.", "content": "In the ongoing NCDS, 44 children of the 46 identified at 7 years as having a serious unilateral hearing loss were followed up at II years. Half of them had recovered normal bilateral hearing, the remainder still had serious deafness in one ear. Although at 7 years the 46 children as a group had shown backwardness in oral ability, speech and reading, at II years both the \"recovered\" and the \"persistent\" were similar to their age peers in scholastic attainment. Despite their original apparent difficulty it was encouraging to find that several children in both subgroups were noted as possessing outstanding academic ability. It is concluded that with prompt follow-up by an alerted school doctor, children with unilateral deafness at age 7 years are likely to progress satisfactorily in later childhood.", "contents": "Follow-up ?at II years of 46 children with severe unilateral hearing loss at 7 years. In the ongoing NCDS, 44 children of the 46 identified at 7 years as having a serious unilateral hearing loss were followed up at II years. Half of them had recovered normal bilateral hearing, the remainder still had serious deafness in one ear. Although at 7 years the 46 children as a group had shown backwardness in oral ability, speech and reading, at II years both the \"recovered\" and the \"persistent\" were similar to their age peers in scholastic attainment. Despite their original apparent difficulty it was encouraging to find that several children in both subgroups were noted as possessing outstanding academic ability. It is concluded that with prompt follow-up by an alerted school doctor, children with unilateral deafness at age 7 years are likely to progress satisfactorily in later childhood.", "PMID": 954156}
{"id": "pubmed23n0032_944", "title": "The effect of the pattern of cardiac sympathetic activity on myocardial contractile force and norepinephrine overflow in the dog heart.", "content": "The left of right cardiac sympathetic nerves in open-chest, anesthetized dogs were stimulated at mean frequencies of 2 or 4 Hz. The stimuli were applied intermittently, in patterns with repetition rates or either 60/min or 15/min, to simulate the spontaneous patterns of sympathetic neural activity that occur synchronously with the cardiac or respiratory cycles, respectively. With either repetition rate, intermittent stimulation of the left sympathetic nerves was about 10-20% less effective in enhancing myocardial contractile force (CF) and about 10% less effective in increasing coronary sinus blood flow than was steady stimulation at the same mean frequency. With right-sided stimulation, there was no appreciable difference between steady and intermettent stimulation patterns with respect to the effect on heart rate. With either left-or right-sided stimulation, the rate of norepinephrine (NE) overflow into the coronary sinus blood was 20-40% less with intermittent than with steady stimulation. Cocaine administration did not materially affect this difference in NE overflow. It was concluded that the higher instantaneous frequencies that prevail during intermittent stimulation result in a reduction in the rate of NE release at the sympathetic postganglionic nerve endings in the heart.", "contents": "The effect of the pattern of cardiac sympathetic activity on myocardial contractile force and norepinephrine overflow in the dog heart. The left of right cardiac sympathetic nerves in open-chest, anesthetized dogs were stimulated at mean frequencies of 2 or 4 Hz. The stimuli were applied intermittently, in patterns with repetition rates or either 60/min or 15/min, to simulate the spontaneous patterns of sympathetic neural activity that occur synchronously with the cardiac or respiratory cycles, respectively. With either repetition rate, intermittent stimulation of the left sympathetic nerves was about 10-20% less effective in enhancing myocardial contractile force (CF) and about 10% less effective in increasing coronary sinus blood flow than was steady stimulation at the same mean frequency. With right-sided stimulation, there was no appreciable difference between steady and intermettent stimulation patterns with respect to the effect on heart rate. With either left-or right-sided stimulation, the rate of norepinephrine (NE) overflow into the coronary sinus blood was 20-40% less with intermittent than with steady stimulation. Cocaine administration did not materially affect this difference in NE overflow. It was concluded that the higher instantaneous frequencies that prevail during intermittent stimulation result in a reduction in the rate of NE release at the sympathetic postganglionic nerve endings in the heart.", "PMID": 954163}
{"id": "pubmed23n0032_945", "title": "Differential methods of enhancing cognitive growth in urban preschool children.", "content": "The investigation was carried out to design and test more purposeful and structured methods of promoting intellectual growth in culturally deprived preschool children. The relative merits of providing specific cognitive training both in a nursery setting and in the home through the influence of the mother were assessed. Four groups each of 15 children were involved in the experiment, which extended over a period of four months. Gains were confirmed for both general intelligence and linguistic competence. Both the two nursery groups and the experimental \"home\" group all showed considerable improvement, the latter being comparable to the nursery control group.", "contents": "Differential methods of enhancing cognitive growth in urban preschool children. The investigation was carried out to design and test more purposeful and structured methods of promoting intellectual growth in culturally deprived preschool children. The relative merits of providing specific cognitive training both in a nursery setting and in the home through the influence of the mother were assessed. Four groups each of 15 children were involved in the experiment, which extended over a period of four months. Gains were confirmed for both general intelligence and linguistic competence. Both the two nursery groups and the experimental \"home\" group all showed considerable improvement, the latter being comparable to the nursery control group.", "PMID": 954154}
{"id": "pubmed23n0032_946", "title": "Interaction of capillary and tissue forces in the cat small intestine.", "content": "We measured steady state capillary hydrostatic pressure (P c,i), plasma and lymph protein concentrations, lymph and blood flow, and capillary filtration coefficients in an in situ loop of cat small intestine at venous outflow pressures (PV) of 0, 5, 10, 15, 20, 25, and 30 mm Hg. The data were used to calculate colloid osmotic pressure of lymph and plasma, interstitial fluid pressure (Pt), pre- and postcapillary resistances, and a tissue pressure-volume curve of the intestinal interstitium. When PV was elevated from 0 to 30 mm Hg, lymph protein concentration decreased from 3.8 to 1.9 g/100 ml (representing a change in colloid osmotic pressure of 6.2 mm Hg), lymph flow increased 7-fold (or an equivalent imbalance in Starling forces of 4.3 mm Hg), and the calculated PT increased from 1.8 to +5.3. Because lymph flow draining the loop decreased during the determination of Pc, i at venous pressures between 15 and 30 mm Hg, the corresponding calculated PT may be in error by 1-2 mm Hg. The tissue pressure-volume relationship calculated from the data indicates that the intestinal interstitial volume expands nonlinearly and this expansion is characterized by two distinctly different compliant components: (1) tissue compliance is low at PV between 0 and 15 mm Hg (0.4 ml/mm Hg), and (2) at PV greater than 15 mm Hg the tissue compliance is relatively high (4 ml/mm Hg). We found that when PV was elevated from 0 to 15 mm Hg, increases in PT are the major tissue adjustments that oppose the increased filtration pressures. Furthermore, at Pv of 20-30 mm Hg, tissue protein concentration decreases, lymph flow relative to the filtration coefficient (deltaP DROP) increases and, to a much lesser extent, PT increases. Finally, the combination of these changes in tissue force at high filtration pressures represent a maximum tissue edema \"safety factor\" of 10 mm Hg; further increases in filtration pressures result in large volume movements into the intestinal lumen.", "contents": "Interaction of capillary and tissue forces in the cat small intestine. We measured steady state capillary hydrostatic pressure (P c,i), plasma and lymph protein concentrations, lymph and blood flow, and capillary filtration coefficients in an in situ loop of cat small intestine at venous outflow pressures (PV) of 0, 5, 10, 15, 20, 25, and 30 mm Hg. The data were used to calculate colloid osmotic pressure of lymph and plasma, interstitial fluid pressure (Pt), pre- and postcapillary resistances, and a tissue pressure-volume curve of the intestinal interstitium. When PV was elevated from 0 to 30 mm Hg, lymph protein concentration decreased from 3.8 to 1.9 g/100 ml (representing a change in colloid osmotic pressure of 6.2 mm Hg), lymph flow increased 7-fold (or an equivalent imbalance in Starling forces of 4.3 mm Hg), and the calculated PT increased from 1.8 to +5.3. Because lymph flow draining the loop decreased during the determination of Pc, i at venous pressures between 15 and 30 mm Hg, the corresponding calculated PT may be in error by 1-2 mm Hg. The tissue pressure-volume relationship calculated from the data indicates that the intestinal interstitial volume expands nonlinearly and this expansion is characterized by two distinctly different compliant components: (1) tissue compliance is low at PV between 0 and 15 mm Hg (0.4 ml/mm Hg), and (2) at PV greater than 15 mm Hg the tissue compliance is relatively high (4 ml/mm Hg). We found that when PV was elevated from 0 to 15 mm Hg, increases in PT are the major tissue adjustments that oppose the increased filtration pressures. Furthermore, at Pv of 20-30 mm Hg, tissue protein concentration decreases, lymph flow relative to the filtration coefficient (deltaP DROP) increases and, to a much lesser extent, PT increases. Finally, the combination of these changes in tissue force at high filtration pressures represent a maximum tissue edema \"safety factor\" of 10 mm Hg; further increases in filtration pressures result in large volume movements into the intestinal lumen.", "PMID": 954164}
{"id": "pubmed23n0032_947", "title": "Sound reward toys.", "content": "The multiplicity of disciplines involved in a comprehensive treatment and assessment centre provides fertile ground for the design of new apparatus of practical use in the field of therapy. Such projects encourage team work, are of considerable benefit to the patients and can lead to new approaches to problems. The construction and use of three sound reward toys for the diagnosis and treatment of speech disorders is described.", "contents": "Sound reward toys. The multiplicity of disciplines involved in a comprehensive treatment and assessment centre provides fertile ground for the design of new apparatus of practical use in the field of therapy. Such projects encourage team work, are of considerable benefit to the patients and can lead to new approaches to problems. The construction and use of three sound reward toys for the diagnosis and treatment of speech disorders is described.", "PMID": 954159}
{"id": "pubmed23n0032_948", "title": "Bed wetting and emotional disorder: an \"interactionist\" model.", "content": "Twenty-two of 94 4-year-olds in a nursery schools sample in a working class area wet their beds at least once a week. The enuresis was significantly associated with other behavioural items which could be interpreted as representing general emotional immaturity. A malaise score of the enuretics' mothers was higher than that of the remaining mothers. It is suggested that from these results can be seen the beginning of a damaging interaction which could lead to emotional disorder in the child.", "contents": "Bed wetting and emotional disorder: an \"interactionist\" model. Twenty-two of 94 4-year-olds in a nursery schools sample in a working class area wet their beds at least once a week. The enuresis was significantly associated with other behavioural items which could be interpreted as representing general emotional immaturity. A malaise score of the enuretics' mothers was higher than that of the remaining mothers. It is suggested that from these results can be seen the beginning of a damaging interaction which could lead to emotional disorder in the child.", "PMID": 954158}
{"id": "pubmed23n0032_949", "title": "Transcapillary escape rate of albumin and right atrial pressure in chronic congestive heart failure before and after treatment.", "content": "The transcapillary escape rate of albumin (TERalb), i.e., the fraction of intravascular mass of albumin that passes to the extravascular space per unit of time, was determined from the disappearance of intravenously injected 125I-labeled human serum albumin during the first 60 minutes after injection in 10 subjects with chronic right heart failure. The investigation was repeated after sodium and water depletion. Before treatment TERalb was significantly elevated (mean 8.3 +/- 1.6% (SD)/hour, in comparison to values for normal subjects (mean 5.4 +/- 1.1%/hour, P less than 0.001). With treatment TERalb decreased significantly (mean 5.9 +/- 1.2%/hour, P less than 0.01). Right atrial pressure decreased from an average of 10 mm Hg to 6 mm Hg during treatment. A statistically significant, positive correlation was found between TERalb and right atrial pressure (r = 0.77, P less than 0.001). Our results best can be explained by increased filtration, mainly through the venous end of the microvasculature, due to the increased venous pressure in heart failure.", "contents": "Transcapillary escape rate of albumin and right atrial pressure in chronic congestive heart failure before and after treatment. The transcapillary escape rate of albumin (TERalb), i.e., the fraction of intravascular mass of albumin that passes to the extravascular space per unit of time, was determined from the disappearance of intravenously injected 125I-labeled human serum albumin during the first 60 minutes after injection in 10 subjects with chronic right heart failure. The investigation was repeated after sodium and water depletion. Before treatment TERalb was significantly elevated (mean 8.3 +/- 1.6% (SD)/hour, in comparison to values for normal subjects (mean 5.4 +/- 1.1%/hour, P less than 0.001). With treatment TERalb decreased significantly (mean 5.9 +/- 1.2%/hour, P less than 0.01). Right atrial pressure decreased from an average of 10 mm Hg to 6 mm Hg during treatment. A statistically significant, positive correlation was found between TERalb and right atrial pressure (r = 0.77, P less than 0.001). Our results best can be explained by increased filtration, mainly through the venous end of the microvasculature, due to the increased venous pressure in heart failure.", "PMID": 954165}
{"id": "pubmed23n0032_950", "title": "Quantification of collateral resistance in acute and chronic experimental coronary occlusion in the dog.", "content": "The resistance to coronary blood flow in various parts of the myocardium was studied with the tracer microspheres technique before and immediately after an acute coronary occlusion and several weeks after a more slowly occurring coronary occlusion by Ameroid constrictor. All experiments were carried out in the isolated, metabolically supported, empty, beating dog heart at maximal coronary vasodilation induced with adenosine. Coronary resistance of the normal empty beating heart at maximal coronary vasodilation was 0.20 mm mm Hg/(ml/min) per 100 g of tissue (subepicardium) and 0.16 mm Hg/(ml/min) per 100 g of tissue (subendocardium). After acute coronary occlusion the perfusion of the subtended myocardium was maintained at a much lower level by way of collateral vessels, which showed a resistance to flow of 3.52 mm Hg/(ml/min) per 100 g. If coronary artery occlusion proceeded more slowly the collateral vessels became more functional and myocardial infarction was avoided. During collateral enlargement collateral resistance fell from 3.52 to 0.22 mm Hg/(ml/min) per 100 g within a period of 8 weeks after implantation of the constricting device. The degree of compensation by collaterals for the loss of the occluded native coronary artery was 33% of its former conductance.", "contents": "Quantification of collateral resistance in acute and chronic experimental coronary occlusion in the dog. The resistance to coronary blood flow in various parts of the myocardium was studied with the tracer microspheres technique before and immediately after an acute coronary occlusion and several weeks after a more slowly occurring coronary occlusion by Ameroid constrictor. All experiments were carried out in the isolated, metabolically supported, empty, beating dog heart at maximal coronary vasodilation induced with adenosine. Coronary resistance of the normal empty beating heart at maximal coronary vasodilation was 0.20 mm mm Hg/(ml/min) per 100 g of tissue (subepicardium) and 0.16 mm Hg/(ml/min) per 100 g of tissue (subendocardium). After acute coronary occlusion the perfusion of the subtended myocardium was maintained at a much lower level by way of collateral vessels, which showed a resistance to flow of 3.52 mm Hg/(ml/min) per 100 g. If coronary artery occlusion proceeded more slowly the collateral vessels became more functional and myocardial infarction was avoided. During collateral enlargement collateral resistance fell from 3.52 to 0.22 mm Hg/(ml/min) per 100 g within a period of 8 weeks after implantation of the constricting device. The degree of compensation by collaterals for the loss of the occluded native coronary artery was 33% of its former conductance.", "PMID": 954167}
{"id": "pubmed23n0032_951", "title": "Effects of treatment with pyruvate and tromethamine in experimental myocardial ischemia.", "content": "Failure of glycolysis to increase sufficiently to supply optimal levels of energy production in ischemic heart muscle is due in part to the cummulative restrainst of acidosis on rate-limiting enzymes, particularly glyceraldehyde-3-phosphate dehydrogenase. In an effort to modify this inhibition and salvage jeopardized myocardium, treatment with excess levels of pyruvate and tromethamine (Tris), designed to buffer intracellular hydrogen ion accumulations and improve the oxidation-reduction ratio, NAD+/NADH, was tested in 59 swine hearts in two separate preparations of global and regional ischemia. Global ischemia, per se, caused hemodynamic deterioration and shortened survival time (44.3 +/- 3.1 minutes). Myocardial oxygen consumption, fatty acid oxidation, and glucose uptake were all significantly (P less than 0.001) reduced as were estimates of glycolysis and tissue stores of creatine phosphate and ATP (P less than 0.01). Although treatment with Tris alone was inconclusive, administrations of pyruvate (40-50 mM) buffered with Tris (added directly into the coronary perfusate) effected an improvement in mechanical function and a significant prolongation in survival time (56.9 +/- 2.6 minutes. P less than 0.01). Glycogenolysis was enhanced and levels of key glycolytic intermediates were reduced, suggesting an acceleration of glycolytic flux. Excess levels of pyruvate (1.52 +/- 0.48 mumol/ml of coronary perfusate) provided added substrate for oxidation and led to a greater than 5-fold incrase in rates of pyruvate decarboxylation as compared to untreated ischemic hearts...", "contents": "Effects of treatment with pyruvate and tromethamine in experimental myocardial ischemia. Failure of glycolysis to increase sufficiently to supply optimal levels of energy production in ischemic heart muscle is due in part to the cummulative restrainst of acidosis on rate-limiting enzymes, particularly glyceraldehyde-3-phosphate dehydrogenase. In an effort to modify this inhibition and salvage jeopardized myocardium, treatment with excess levels of pyruvate and tromethamine (Tris), designed to buffer intracellular hydrogen ion accumulations and improve the oxidation-reduction ratio, NAD+/NADH, was tested in 59 swine hearts in two separate preparations of global and regional ischemia. Global ischemia, per se, caused hemodynamic deterioration and shortened survival time (44.3 +/- 3.1 minutes). Myocardial oxygen consumption, fatty acid oxidation, and glucose uptake were all significantly (P less than 0.001) reduced as were estimates of glycolysis and tissue stores of creatine phosphate and ATP (P less than 0.01). Although treatment with Tris alone was inconclusive, administrations of pyruvate (40-50 mM) buffered with Tris (added directly into the coronary perfusate) effected an improvement in mechanical function and a significant prolongation in survival time (56.9 +/- 2.6 minutes. P less than 0.01). Glycogenolysis was enhanced and levels of key glycolytic intermediates were reduced, suggesting an acceleration of glycolytic flux. Excess levels of pyruvate (1.52 +/- 0.48 mumol/ml of coronary perfusate) provided added substrate for oxidation and led to a greater than 5-fold incrase in rates of pyruvate decarboxylation as compared to untreated ischemic hearts...", "PMID": 954168}
{"id": "pubmed23n0032_952", "title": "The decay of the potentiated state in sheep and calf ventricular myocardial fibers. Influence of agents acting on transmembrane Ca2+ flux.", "content": "Paired pulse stimulation increases the contractile strength of mammalian myocardium. If stimulation is discontinued the \"potentiated state\" takes several minutes to decay, as shown by the first contraction following resumption of stimulation. From earlier experiments it has been inferred that contractile force depends on Ca2+ released from intracellular stores rather than on an influx of Ca2+ associated with a given action potential. This view now receives support from the following findings: (1) Ni2+ and Co2+, known to inhibit Ca2+ influx during the action potential, when applied during a 2-minute rest period, are practically without effect on the strength of the first beat but strongly depress steady state contractions. (2) Caffeine, known to enhance Ca2+ efflux from and inhibit Ca2+ uptake into intracellular stores, greatly accelerates the decay of the potentiated state during a rest period. (3) Na+ -poor solution, known to inhibit Ca2+ efflux, has a strong positive inotropic effect. Paired pulse stimulation fails to increase contractile strength in Na+ -poor solution, and a rest period of many minutes is practically without effect on the amplitude of the first beat after resumption of stimulation. The results indicate that contraction is due to Ca2+ released from internal stores whose degree of filling can be altered.", "contents": "The decay of the potentiated state in sheep and calf ventricular myocardial fibers. Influence of agents acting on transmembrane Ca2+ flux. Paired pulse stimulation increases the contractile strength of mammalian myocardium. If stimulation is discontinued the \"potentiated state\" takes several minutes to decay, as shown by the first contraction following resumption of stimulation. From earlier experiments it has been inferred that contractile force depends on Ca2+ released from intracellular stores rather than on an influx of Ca2+ associated with a given action potential. This view now receives support from the following findings: (1) Ni2+ and Co2+, known to inhibit Ca2+ influx during the action potential, when applied during a 2-minute rest period, are practically without effect on the strength of the first beat but strongly depress steady state contractions. (2) Caffeine, known to enhance Ca2+ efflux from and inhibit Ca2+ uptake into intracellular stores, greatly accelerates the decay of the potentiated state during a rest period. (3) Na+ -poor solution, known to inhibit Ca2+ efflux, has a strong positive inotropic effect. Paired pulse stimulation fails to increase contractile strength in Na+ -poor solution, and a rest period of many minutes is practically without effect on the amplitude of the first beat after resumption of stimulation. The results indicate that contraction is due to Ca2+ released from internal stores whose degree of filling can be altered.", "PMID": 954169}
{"id": "pubmed23n0032_953", "title": "Chronic one-kidney hypertension in rabbits. II. Evidence for a new factor.", "content": "The arterial pressure of rabbits with chronic one-kidney hypertension can be lowered to normotensive levels by direct immunization with preparations made from the cortex of hog kidneys. Hypertensive rabbits that are immunized with large amounts of renin may develop high plasma antirenin titers without affecting their blood pressure. Removal of renin by chromatography on columns of immobilized antirenin yields preparations with little or no renin. Such preparations may be effective in lowering blood pressure although they do not elicit the formation of plasma antirenin and the rabbits remain sensitive to challenging doses of rabbit renin. The evidence suggests that an unknown substance is responsible for the development of a cross-reacting antibody that neutralizes an unknown factor that is essential for the maintence of an elevated blood pressure level in rabbits with chronic one-kidney hypertension.", "contents": "Chronic one-kidney hypertension in rabbits. II. Evidence for a new factor. The arterial pressure of rabbits with chronic one-kidney hypertension can be lowered to normotensive levels by direct immunization with preparations made from the cortex of hog kidneys. Hypertensive rabbits that are immunized with large amounts of renin may develop high plasma antirenin titers without affecting their blood pressure. Removal of renin by chromatography on columns of immobilized antirenin yields preparations with little or no renin. Such preparations may be effective in lowering blood pressure although they do not elicit the formation of plasma antirenin and the rabbits remain sensitive to challenging doses of rabbit renin. The evidence suggests that an unknown substance is responsible for the development of a cross-reacting antibody that neutralizes an unknown factor that is essential for the maintence of an elevated blood pressure level in rabbits with chronic one-kidney hypertension.", "PMID": 954170}
{"id": "pubmed23n0032_954", "title": "Age-related changes in ouabain pharmacology. Ouabain exhibits a different volume of distribution in adult and young dogs.", "content": "To better understand why one must administer much higher doeses of digitalis glycosides to immature than to mature humans and animals, we studied ouabain pharmacokinetics in adult and young dogs. Consistent with reported observations that ouabain-binding, metabolism, and excretion do not change with age, we found no significant differences in the transfer coefficients in a linear two-compartment open model for ouabain pharmacokinetics following a bolus of 0.05 mg/kg. We did find, however, that young dogs had nearly twice the ouabain volumes of distribution per kilogram of body weight as adults (155.4 +/- 1.2 (SE) ml/kg vs. 80 +/- 0.6, P less than 0.0005) and that one could account for this difference with the fact that young dogs had nearly twice the plasma volume 108 +/- 9.8, vs. 68 +/- 7.3, P = 0.001) and interstitial fluid space (318 +/- 35 vs. 190 +/- 6.5, P = 0.006) as the adults. For the same dose per kilogram, left and right ventricular ouabain concentrations were inversely related to the volume of distribution, with the adults having significantly higher tissue levels and incidence of arrhythmias. One must give more ouabain to a young dog to get the same plasma concentration as in an adult because the mass of ouabain in rapid equilibrium with the plasma is diluted in a larger volume of distribution.", "contents": "Age-related changes in ouabain pharmacology. Ouabain exhibits a different volume of distribution in adult and young dogs. To better understand why one must administer much higher doeses of digitalis glycosides to immature than to mature humans and animals, we studied ouabain pharmacokinetics in adult and young dogs. Consistent with reported observations that ouabain-binding, metabolism, and excretion do not change with age, we found no significant differences in the transfer coefficients in a linear two-compartment open model for ouabain pharmacokinetics following a bolus of 0.05 mg/kg. We did find, however, that young dogs had nearly twice the ouabain volumes of distribution per kilogram of body weight as adults (155.4 +/- 1.2 (SE) ml/kg vs. 80 +/- 0.6, P less than 0.0005) and that one could account for this difference with the fact that young dogs had nearly twice the plasma volume 108 +/- 9.8, vs. 68 +/- 7.3, P = 0.001) and interstitial fluid space (318 +/- 35 vs. 190 +/- 6.5, P = 0.006) as the adults. For the same dose per kilogram, left and right ventricular ouabain concentrations were inversely related to the volume of distribution, with the adults having significantly higher tissue levels and incidence of arrhythmias. One must give more ouabain to a young dog to get the same plasma concentration as in an adult because the mass of ouabain in rapid equilibrium with the plasma is diluted in a larger volume of distribution.", "PMID": 954171}
{"id": "pubmed23n0032_955", "title": "Effect of exercise conditioning on the intrinsic contractile state of cat myocardium.", "content": "Fourteen adult cats were exercised on a motor-driven treadmill 5 days each week for 6 weeks to determine the effect of exercise conditioning on the intrinsic contractile state of the myocardium. The exercise program was sufficient to produce a cardiovascular training effect manifested by slower exercising heart rates and resting heart rates after atropine by the end of the 6th week. The mechanical function of the isolated right ventricular papillary muscle from exercised cats was compared with that of 17 sedentary adult cats. There were no significant differences between exercised and control cats in in heart weight-body weight ratios, resting and active lenght - tension relations, maximal rate of isometric force development at the peak of the length-tension curve (Lmax), time to peak force at Lmax, maximal force development with paired stimulation and norepinephrine, or force-velocity relations. These results indicate that the intrinsic state of feline myocardium is unaffected by exercise conditioning.", "contents": "Effect of exercise conditioning on the intrinsic contractile state of cat myocardium. Fourteen adult cats were exercised on a motor-driven treadmill 5 days each week for 6 weeks to determine the effect of exercise conditioning on the intrinsic contractile state of the myocardium. The exercise program was sufficient to produce a cardiovascular training effect manifested by slower exercising heart rates and resting heart rates after atropine by the end of the 6th week. The mechanical function of the isolated right ventricular papillary muscle from exercised cats was compared with that of 17 sedentary adult cats. There were no significant differences between exercised and control cats in in heart weight-body weight ratios, resting and active lenght - tension relations, maximal rate of isometric force development at the peak of the length-tension curve (Lmax), time to peak force at Lmax, maximal force development with paired stimulation and norepinephrine, or force-velocity relations. These results indicate that the intrinsic state of feline myocardium is unaffected by exercise conditioning.", "PMID": 954172}
{"id": "pubmed23n0032_956", "title": "Effects of cardiac sympathetic stimulation and ablation of canine ventricular anodal strength-interval curves.", "content": "We studied the effects of cardiac sympathetic stimulation and ablation on characteristics of the anodal-strength interval curve in 10 pentobarbital-anesthetized open-chest dogs. We gave special attention to the form and particularly the depth of early dips of the anodal strength-interval curve. When the central connections of the cardiac sympathetic nerves were intact, left stellate stimulation decreased threshold for excitation during the dip only slightly (from 883 +/- 442 muA to 774 +/- 326 muA P less than 0.1). However, after left stellectomy and upper thoracic ganglionectomy, stimulation of the cut ends the ansa subclavia decreased the dip threshold from an average of 952 +/- 321 muA to 707 +/- 210 muA, A less than 0.01. Dip thresholds in strength-interval curves determined 1-1.5 hours after ganglionectomy average 1164 +/- 296 muA compared to an average dip threshold of 791 +/- 269 muA in strength-interval curves measured immediately prior to stellectomy. This increase is statistically significant (P less than 0.005). In addition to the effects of sympathetic tone on disparity of refractory period and ventricular fibrillation threshold, the effects of sympathetic tone on the form of the strength-interval curve may be another factor influencing vulnerability to arrhythmias and particularly in patients with abnormal autonomic tone.", "contents": "Effects of cardiac sympathetic stimulation and ablation of canine ventricular anodal strength-interval curves. We studied the effects of cardiac sympathetic stimulation and ablation on characteristics of the anodal-strength interval curve in 10 pentobarbital-anesthetized open-chest dogs. We gave special attention to the form and particularly the depth of early dips of the anodal strength-interval curve. When the central connections of the cardiac sympathetic nerves were intact, left stellate stimulation decreased threshold for excitation during the dip only slightly (from 883 +/- 442 muA to 774 +/- 326 muA P less than 0.1). However, after left stellectomy and upper thoracic ganglionectomy, stimulation of the cut ends the ansa subclavia decreased the dip threshold from an average of 952 +/- 321 muA to 707 +/- 210 muA, A less than 0.01. Dip thresholds in strength-interval curves determined 1-1.5 hours after ganglionectomy average 1164 +/- 296 muA compared to an average dip threshold of 791 +/- 269 muA in strength-interval curves measured immediately prior to stellectomy. This increase is statistically significant (P less than 0.005). In addition to the effects of sympathetic tone on disparity of refractory period and ventricular fibrillation threshold, the effects of sympathetic tone on the form of the strength-interval curve may be another factor influencing vulnerability to arrhythmias and particularly in patients with abnormal autonomic tone.", "PMID": 954173}
{"id": "pubmed23n0032_957", "title": "Cell permeability, sodium transport, and the hypertensive process in the rat.", "content": "The tail artery of the spontaneously hypertensive rat (SHR) (Carworth Farms), excised rapidly and immersed immediately in cold (2 degrees C) Li-substituted physiologic salt solution (LiPSS), continues to exchange cell Na+ and K+ for Li+, this exchange is negligible to the control (Carworth Farms normotensive) CFN). In the incubated artery at 37 degrees C, when the vascular smooth muscle cell is slack, the leakiness of the cell membrane in the SHR is more than offset by increased Na+ pumping activity, so that cell Na+ is subnormal. A high precision technique with ion-specific electrodes was developed to follow the passive downhill and active uphill phases of Na+-K+ exchange in the perfused artery exposed to K+-free physiologic salt solution (K+-free PSS) followed by physiological salt solution (PSS). The exchange was found to be fully reversible and sufficiently equimolar to be definable in terms of movements of K+ alone. The rates of ionic movement across the vascular smooth muscle cell were found to be about 6 times faster for the vessel perfused at low pressure (less than 3 mm Hg) than for the slack incubated artery. The rate of passive downhill movement was significantly accelerated in the mature SHR compared with CFN, and the net active transport activity much enhanced. Similar changes were seen as early as 3 weeks after treatment with DOCA and were pronounced at 8 weeks. It is proposed that conditions favoring a sustained accumulation of Na+ in the vascular smooth muscle cell are countered by an enhanced synthesis of transport protein, of contractile protein, and of paracellular matrix protein which progressively restructure the wall.", "contents": "Cell permeability, sodium transport, and the hypertensive process in the rat. The tail artery of the spontaneously hypertensive rat (SHR) (Carworth Farms), excised rapidly and immersed immediately in cold (2 degrees C) Li-substituted physiologic salt solution (LiPSS), continues to exchange cell Na+ and K+ for Li+, this exchange is negligible to the control (Carworth Farms normotensive) CFN). In the incubated artery at 37 degrees C, when the vascular smooth muscle cell is slack, the leakiness of the cell membrane in the SHR is more than offset by increased Na+ pumping activity, so that cell Na+ is subnormal. A high precision technique with ion-specific electrodes was developed to follow the passive downhill and active uphill phases of Na+-K+ exchange in the perfused artery exposed to K+-free physiologic salt solution (K+-free PSS) followed by physiological salt solution (PSS). The exchange was found to be fully reversible and sufficiently equimolar to be definable in terms of movements of K+ alone. The rates of ionic movement across the vascular smooth muscle cell were found to be about 6 times faster for the vessel perfused at low pressure (less than 3 mm Hg) than for the slack incubated artery. The rate of passive downhill movement was significantly accelerated in the mature SHR compared with CFN, and the net active transport activity much enhanced. Similar changes were seen as early as 3 weeks after treatment with DOCA and were pronounced at 8 weeks. It is proposed that conditions favoring a sustained accumulation of Na+ in the vascular smooth muscle cell are countered by an enhanced synthesis of transport protein, of contractile protein, and of paracellular matrix protein which progressively restructure the wall.", "PMID": 954174}
{"id": "pubmed23n0032_958", "title": "Hormonal and nutritional substrate control of cardiac lysosomal enzyme activities.", "content": "Prolonged starvation is known to induce significant alterations in several cardiac lysosomal enzymes, particularly the acid proteinase cathepsin D. To determine what specific factors might mediate these changes, fetal mouse hearts in organ culture were maintained in media designed to simulate selected hormonal or nutritional substrate changes that accompany starvation. Reduced concentrations of glucose caused an increase in the activity of beta-acetylglucosaminidase but had no effect on cathepsin D or acid phosphatase activites (i.e., effects opposite from those of starvation). Also, high concentrations of free fatty acid, acetoacetate, and beta-OH-butyrate induced an increase in cathepsin D (+18%) and a simultaneous decrease in glucosaminidase (-19%), with little change in acid phosphatase. Furthermore, glucagon had no effect on any of the enzymes, whereas growth hormone caused a small (6%) increase in cathepsin D activity. In addition, insulin deprivation caused significant increases (7-25%) in the activities of all three enzymes. Insulin deprivation and excess ketones, but not the other interventions, increased the proportion of enzyme activity which was nonsedimentable. These results suggest the possibility that lysosomal alterations during starvation may be related in part to prolonged insulin deficiency and exposure to high concentrations of ketones and free fatty acids.", "contents": "Hormonal and nutritional substrate control of cardiac lysosomal enzyme activities. Prolonged starvation is known to induce significant alterations in several cardiac lysosomal enzymes, particularly the acid proteinase cathepsin D. To determine what specific factors might mediate these changes, fetal mouse hearts in organ culture were maintained in media designed to simulate selected hormonal or nutritional substrate changes that accompany starvation. Reduced concentrations of glucose caused an increase in the activity of beta-acetylglucosaminidase but had no effect on cathepsin D or acid phosphatase activites (i.e., effects opposite from those of starvation). Also, high concentrations of free fatty acid, acetoacetate, and beta-OH-butyrate induced an increase in cathepsin D (+18%) and a simultaneous decrease in glucosaminidase (-19%), with little change in acid phosphatase. Furthermore, glucagon had no effect on any of the enzymes, whereas growth hormone caused a small (6%) increase in cathepsin D activity. In addition, insulin deprivation caused significant increases (7-25%) in the activities of all three enzymes. Insulin deprivation and excess ketones, but not the other interventions, increased the proportion of enzyme activity which was nonsedimentable. These results suggest the possibility that lysosomal alterations during starvation may be related in part to prolonged insulin deficiency and exposure to high concentrations of ketones and free fatty acids.", "PMID": 954175}
{"id": "pubmed23n0032_959", "title": "Red cell velocity and plasma transit time in the cerebral microcirculation of spherocytic deer mice.", "content": "Spherocytic deer mice provide a model of human spherocytosis. Their erythrocytes are abnormal in shape and are more rigid than normal red blood cells (RBC). Like their human counterparts, spherocytic mice are anemic. Measurements of RBC velocity in microvessels on the cerebral surface failed to reveal a difference between the velocity of cells in spherocytic as compared to normal deer mice. However, plasma transit, as measured by fluorescein microangiography, was faster than normal. Both decreased plasma transit time and increased RBC velocity are expected in nonspherocytic, anemic mice. Since the former, but not the latter, was found in the spherocytic, anemic mice, it appears that increased RBC rigidity has a greater effect on RBC movement than on plasma movement within the cerebral microcirculation. Thus iit would seem that this increased RBC rigidity prevents the increased RBC velocity that otherwise would be observed in anemia but does not prevent the decrease in plasma transit time.", "contents": "Red cell velocity and plasma transit time in the cerebral microcirculation of spherocytic deer mice. Spherocytic deer mice provide a model of human spherocytosis. Their erythrocytes are abnormal in shape and are more rigid than normal red blood cells (RBC). Like their human counterparts, spherocytic mice are anemic. Measurements of RBC velocity in microvessels on the cerebral surface failed to reveal a difference between the velocity of cells in spherocytic as compared to normal deer mice. However, plasma transit, as measured by fluorescein microangiography, was faster than normal. Both decreased plasma transit time and increased RBC velocity are expected in nonspherocytic, anemic mice. Since the former, but not the latter, was found in the spherocytic, anemic mice, it appears that increased RBC rigidity has a greater effect on RBC movement than on plasma movement within the cerebral microcirculation. Thus iit would seem that this increased RBC rigidity prevents the increased RBC velocity that otherwise would be observed in anemia but does not prevent the decrease in plasma transit time.", "PMID": 954176}
{"id": "pubmed23n0032_960", "title": "Effect of different lysing and washing methods on free amino acid concentrations of sheep whole blood and erythrocytes.", "content": "1. A study was conducted to determine the effect of different washing and lysing methods on the free amino acid concentrations of sheep erythryocytes (RBC) and whole blood. Methods of lysis included laking, freeze-thawing or sonication. RBC were washed with isotonic solutions of either saline or sucrose. 2. Washing the RBC with isotonic sucrose resulted in significantly greater concentrations for ten of the amino acids. Greater amino acid concentrations were realized when washed RBC were sonicated and when the RBC of whole blood were lysed by laking. 3. Suggestions are made for future application to whole blood and RBC amino acid determinations.", "contents": "Effect of different lysing and washing methods on free amino acid concentrations of sheep whole blood and erythrocytes. 1. A study was conducted to determine the effect of different washing and lysing methods on the free amino acid concentrations of sheep erythryocytes (RBC) and whole blood. Methods of lysis included laking, freeze-thawing or sonication. RBC were washed with isotonic solutions of either saline or sucrose. 2. Washing the RBC with isotonic sucrose resulted in significantly greater concentrations for ten of the amino acids. Greater amino acid concentrations were realized when washed RBC were sonicated and when the RBC of whole blood were lysed by laking. 3. Suggestions are made for future application to whole blood and RBC amino acid determinations.", "PMID": 954179}
{"id": "pubmed23n0032_961", "title": "The effects of anti-rheumatoid drugs on the in vitro activity of human serum hyaluronidase.", "content": "Fourteen antirheumatoid drugs were tested for their effects on the in vitro hyaluronidase activity of normal human serum. Four drugs produced significant changes in enzyme activity. Different results were obtained with ovine testicular hyaluronidase when diluted with either saline or inactivated human serum. No increase in serum hyaluronidase activity was found in patients with rheumatoid arthritis. There was no evidence for the existence of tissue specific isoenzymes of hyaluronidase in the serum of either normal subjects or patients with rheumatoid arthritis.", "contents": "The effects of anti-rheumatoid drugs on the in vitro activity of human serum hyaluronidase. Fourteen antirheumatoid drugs were tested for their effects on the in vitro hyaluronidase activity of normal human serum. Four drugs produced significant changes in enzyme activity. Different results were obtained with ovine testicular hyaluronidase when diluted with either saline or inactivated human serum. No increase in serum hyaluronidase activity was found in patients with rheumatoid arthritis. There was no evidence for the existence of tissue specific isoenzymes of hyaluronidase in the serum of either normal subjects or patients with rheumatoid arthritis.", "PMID": 954180}
{"id": "pubmed23n0032_962", "title": "The quantitation of urinary and spinal fluid proteins by a modified biuret method.", "content": "A method is presented for the quantitation of urinary and CSF protein. In the presence of 0.10 N NaOH the peptide bonds of the protein remove and bind copper from an ion exchange resin. The resulting copper-protein complex is separated from low molecular weight substances by gel filtration and the copper in the eluted complex is determined colorimetrically with diethyldithiocarbamic acid. The method requires only 100mul of sample, has biuret specificity and uses a single prepacked column. The limit of sensitivity is 2 mg of protein per deciliter.", "contents": "The quantitation of urinary and spinal fluid proteins by a modified biuret method. A method is presented for the quantitation of urinary and CSF protein. In the presence of 0.10 N NaOH the peptide bonds of the protein remove and bind copper from an ion exchange resin. The resulting copper-protein complex is separated from low molecular weight substances by gel filtration and the copper in the eluted complex is determined colorimetrically with diethyldithiocarbamic acid. The method requires only 100mul of sample, has biuret specificity and uses a single prepacked column. The limit of sensitivity is 2 mg of protein per deciliter.", "PMID": 954181}
{"id": "pubmed23n0032_963", "title": "A comparative study of micro-ammonia determinations in plasma using two different methods.", "content": "Two commercially available kits for determining blood ammonia were compared, an enzymatic method requiring a sample volume of 100mul and a nonenzymatic method requiring 1 ml of sample. Calibration curves for both kits were linear to 270 mumol/l, however correlation studies on plasma samples revealed a lack of agreement between methods (r equal to 0.825, Y equal to 0.76X +51). Improved correlation occurs when protein interference is eliminated from the enzymatic procedure. Finally, the nonenzymatic method is modified to accommodate 100 mul of sample and compared to the original method.", "contents": "A comparative study of micro-ammonia determinations in plasma using two different methods. Two commercially available kits for determining blood ammonia were compared, an enzymatic method requiring a sample volume of 100mul and a nonenzymatic method requiring 1 ml of sample. Calibration curves for both kits were linear to 270 mumol/l, however correlation studies on plasma samples revealed a lack of agreement between methods (r equal to 0.825, Y equal to 0.76X +51). Improved correlation occurs when protein interference is eliminated from the enzymatic procedure. Finally, the nonenzymatic method is modified to accommodate 100 mul of sample and compared to the original method.", "PMID": 954182}
{"id": "pubmed23n0032_964", "title": "A simple method for \"reference range\" estimation from routine laboratory data.", "content": "A simplified method is presented to estimate reference ranges from hospital laboratory data. It is based on a combination of graphical estimation of relative sizes of normal and abnormal populations and the \"mode-center\" concept in which the mode of the total population centers on the 50% cumulative frequency of the normal population. This method can be applied to determine reference ranges even though the data source contains abnormally high and/or low values. The reference ranges obtained for BUN and calcium from in-patient and out-patient sources by this method were found to be similar to those reported for \"healthy\" subjects.", "contents": "A simple method for \"reference range\" estimation from routine laboratory data. A simplified method is presented to estimate reference ranges from hospital laboratory data. It is based on a combination of graphical estimation of relative sizes of normal and abnormal populations and the \"mode-center\" concept in which the mode of the total population centers on the 50% cumulative frequency of the normal population. This method can be applied to determine reference ranges even though the data source contains abnormally high and/or low values. The reference ranges obtained for BUN and calcium from in-patient and out-patient sources by this method were found to be similar to those reported for \"healthy\" subjects.", "PMID": 954183}
{"id": "pubmed23n0032_965", "title": "Critical evaluation of the logit-log plot for the radio assay of vitamin B12.", "content": "The dose-response calculation for radio assay of vitamin B12 is carried out using a straight line and a third degree curve to fit the experimentally obtained calibration points. It is shown that high dispersion of the calibration points with respect to the regression line can lead to errors in the calculation of the response. The calculation of the mean dispersion sx with respect to the regression line turns out to be a good criterion for checking the accuracy of the results.", "contents": "Critical evaluation of the logit-log plot for the radio assay of vitamin B12. The dose-response calculation for radio assay of vitamin B12 is carried out using a straight line and a third degree curve to fit the experimentally obtained calibration points. It is shown that high dispersion of the calibration points with respect to the regression line can lead to errors in the calculation of the response. The calculation of the mean dispersion sx with respect to the regression line turns out to be a good criterion for checking the accuracy of the results.", "PMID": 954184}
{"id": "pubmed23n0032_966", "title": "On the interpretation and potential diagnostic value of the measurements related to lecithin:cholesterol acyltransferase activity.", "content": "At least one reliable method is available for measuring LCAT activity in human subjects(25), and this assay may help to probe even subtle changes in plasma lipids and lipoproteins. Since plasma lipids and lipoproteins are known to be present in abnormal amounts in a large number of metabolic diseases, the method of measuring the rate of cholesterol esterification in plasma could assume a substantial role in the diagnosis and (or) monitoring of treatment of pathologic states.", "contents": "On the interpretation and potential diagnostic value of the measurements related to lecithin:cholesterol acyltransferase activity. At least one reliable method is available for measuring LCAT activity in human subjects(25), and this assay may help to probe even subtle changes in plasma lipids and lipoproteins. Since plasma lipids and lipoproteins are known to be present in abnormal amounts in a large number of metabolic diseases, the method of measuring the rate of cholesterol esterification in plasma could assume a substantial role in the diagnosis and (or) monitoring of treatment of pathologic states.", "PMID": 954185}
{"id": "pubmed23n0032_967", "title": "Comparison of two radioimmunoassay kits for aldosterone determination.", "content": "Two commercially available radioimmunoassay kits for aldosterone determination not requiring preliminary chromatographic purification were examined. Aldosterone standard curve solutions prepared from the CIS kit, Nuclear International Corp., (x-axis) and Diagnostic Products kit (y-axis) were assayed with both kits and the results yielded a regression equation of y = 0.834x + 0.08 nmol/liter. Analytical recovery experiments with aldosterone added to urine and serum showed 120% and 101% recovery with the CIS kit, respectively, and 92% and 92% recovery with the Diagnostic Products kit, respectively. Both kits demonstrated good parallelism with urine and serum. Antibody specificity was tested with six structurally related steroids and each kit showed virtually no cross-reactivity at clinically normal serum concentrations. Serum aldosterone values were compared before (y-axis) and after (x-axis) column chromatography on Sephadex LH-20, with the following regression equations: y = 1.070x + 0.092 nmol/liter for the CIS kit and y = 1.023x + 0.093 nmol/liter for the Diagnostic Products kit. Regression equations comparing patient samples between kits, CIS (x-axis) and Diagnostic Products (y-axis), were: y = 0.832x + 0.007 mumol/24 h with urine and y = 0.850x + 0.097 nmol/liter with serum.", "contents": "Comparison of two radioimmunoassay kits for aldosterone determination. Two commercially available radioimmunoassay kits for aldosterone determination not requiring preliminary chromatographic purification were examined. Aldosterone standard curve solutions prepared from the CIS kit, Nuclear International Corp., (x-axis) and Diagnostic Products kit (y-axis) were assayed with both kits and the results yielded a regression equation of y = 0.834x + 0.08 nmol/liter. Analytical recovery experiments with aldosterone added to urine and serum showed 120% and 101% recovery with the CIS kit, respectively, and 92% and 92% recovery with the Diagnostic Products kit, respectively. Both kits demonstrated good parallelism with urine and serum. Antibody specificity was tested with six structurally related steroids and each kit showed virtually no cross-reactivity at clinically normal serum concentrations. Serum aldosterone values were compared before (y-axis) and after (x-axis) column chromatography on Sephadex LH-20, with the following regression equations: y = 1.070x + 0.092 nmol/liter for the CIS kit and y = 1.023x + 0.093 nmol/liter for the Diagnostic Products kit. Regression equations comparing patient samples between kits, CIS (x-axis) and Diagnostic Products (y-axis), were: y = 0.832x + 0.007 mumol/24 h with urine and y = 0.850x + 0.097 nmol/liter with serum.", "PMID": 954186}
{"id": "pubmed23n0032_968", "title": "Kinetic urine creatinine determination with the Gemsaec analyzer.", "content": "A rapid automated method for urinary creatinine with the Jaff\u00e9 reaction is described. Interferences by protein, glucose and ketones are circumvented by the reaction rate kinetics. The precision and accuracy of this centrifugal method on the ENI-Gemsaec analyzer are comparable to the manual end-point Jaff\u00e9 reaction with deproteinization and to the common Auto-Analyzer technique.", "contents": "Kinetic urine creatinine determination with the Gemsaec analyzer. A rapid automated method for urinary creatinine with the Jaff\u00e9 reaction is described. Interferences by protein, glucose and ketones are circumvented by the reaction rate kinetics. The precision and accuracy of this centrifugal method on the ENI-Gemsaec analyzer are comparable to the manual end-point Jaff\u00e9 reaction with deproteinization and to the common Auto-Analyzer technique.", "PMID": 954187}
{"id": "pubmed23n0032_969", "title": "A minicomputer-automated array spectrometer for liquid-chromatographic detection of metabolites.", "content": "A third-generation multiwavelength array spectrometer was developed as a detector for the high-resolution liquid-chromatographic characterization of metabolites. Components include a PDP-8/e minicomputer, matched pair of linear photodiode arrays, holographically-ruled gratings, fiber optics, flow cells, and high intensity xenon light source. The wavelength range is 256 nm differential with 1-nm resolution and can be adjusted from 200 to 800 nm. The system is capable of storing 20 spectra per second (200-456 nm) in a dual-beam mode. Special features include minicomputer-driven signal enhancement via integration as a function of signal strength. The display output includes presentation of the total absorption chromatogram vs. elution time in both real and post-run time as well as selectable single absorption band vs. elution time (post-run time). Application of this dedicated system is illustrated by the separation and charcterization of the metabolites of a carcinogen, 4-ethylsulfonyl-1 napthalenesulfonamide.", "contents": "A minicomputer-automated array spectrometer for liquid-chromatographic detection of metabolites. A third-generation multiwavelength array spectrometer was developed as a detector for the high-resolution liquid-chromatographic characterization of metabolites. Components include a PDP-8/e minicomputer, matched pair of linear photodiode arrays, holographically-ruled gratings, fiber optics, flow cells, and high intensity xenon light source. The wavelength range is 256 nm differential with 1-nm resolution and can be adjusted from 200 to 800 nm. The system is capable of storing 20 spectra per second (200-456 nm) in a dual-beam mode. Special features include minicomputer-driven signal enhancement via integration as a function of signal strength. The display output includes presentation of the total absorption chromatogram vs. elution time in both real and post-run time as well as selectable single absorption band vs. elution time (post-run time). Application of this dedicated system is illustrated by the separation and charcterization of the metabolites of a carcinogen, 4-ethylsulfonyl-1 napthalenesulfonamide.", "PMID": 954189}
{"id": "pubmed23n0032_970", "title": "Ion chromatography: a new technique for clinical chemistry.", "content": "Ion chromatography is a new analytical technique in which the ions to be analyzed are separated on a special pellicular ion-exchange column. Once separated, the effluent from the separating column passes into a second ion-exchange column, which removes the eluent or buffer ions from the moving phase without removing the sample ions, which are detected and measured by electrical conductivity on a background of low conductance. Trace quantities can be so determined. This technique has been applied to four biological fluids: blood serum, tissue extract, urine, and cerebrospinal fluid. It is shown to be a practical analytical tool, particularly for ions having no chromophores, such as sodium, ammonium, potassium, magensium, calcium, chloride, nitrite, phosphate, and sulfate.", "contents": "Ion chromatography: a new technique for clinical chemistry. Ion chromatography is a new analytical technique in which the ions to be analyzed are separated on a special pellicular ion-exchange column. Once separated, the effluent from the separating column passes into a second ion-exchange column, which removes the eluent or buffer ions from the moving phase without removing the sample ions, which are detected and measured by electrical conductivity on a background of low conductance. Trace quantities can be so determined. This technique has been applied to four biological fluids: blood serum, tissue extract, urine, and cerebrospinal fluid. It is shown to be a practical analytical tool, particularly for ions having no chromophores, such as sodium, ammonium, potassium, magensium, calcium, chloride, nitrite, phosphate, and sulfate.", "PMID": 954190}
{"id": "pubmed23n0032_971", "title": "An immobilized-enzyme flow-enthalpimetric analyzer: application to glucose determination by direct phosphorylation catalyzed by hexokinase.", "content": "A novel flow-enthalpimetric analyzer is described and its use demonstrated by an analysis in which glucose is determined by its hexokinase-catalyzed phosphorylation reaction. The method depends on measurement of the temperature differential across a column packed with glass-supported immoblized enzyme. Sample volumes of 120 mul can be used to obtain a calibration curve that is linear up to 25 mmol of glucose per liter. A precision (within-day) of 5% is generally observed in the optimum concentration range where glucose is quantitatively phosphorylated. Results by the technique correlate reasonably with those by the o-toluidine and the hexokinase/glucose-6-phosphate dehydrogenase methods: Other sugars--including fructose, glucosamine, and mannose--will interfere; galactose does not. The technique is amenable to both routine and emergency analyses.", "contents": "An immobilized-enzyme flow-enthalpimetric analyzer: application to glucose determination by direct phosphorylation catalyzed by hexokinase. A novel flow-enthalpimetric analyzer is described and its use demonstrated by an analysis in which glucose is determined by its hexokinase-catalyzed phosphorylation reaction. The method depends on measurement of the temperature differential across a column packed with glass-supported immoblized enzyme. Sample volumes of 120 mul can be used to obtain a calibration curve that is linear up to 25 mmol of glucose per liter. A precision (within-day) of 5% is generally observed in the optimum concentration range where glucose is quantitatively phosphorylated. Results by the technique correlate reasonably with those by the o-toluidine and the hexokinase/glucose-6-phosphate dehydrogenase methods: Other sugars--including fructose, glucosamine, and mannose--will interfere; galactose does not. The technique is amenable to both routine and emergency analyses.", "PMID": 954191}
{"id": "pubmed23n0032_972", "title": "The free-falling drop detector--a nover fluorescence detector for high-performance liquid chromatography.", "content": "A novel fluorescence detector for high-performance liquid chromatography has been designed to minimize the instrumental limitations of conventional flow-through, fluorometers. This unit contributes less than 5 mul to the \"dead volume\" of the chromatographic system. Sensitivity comparable to that of existing flow-through fluorometers has been attained without optimizing instrumental parameters. Performance of the unit has been evaluated with reference samples containing saccharides typical of glycosphingolipids. Future improvements are discussed.", "contents": "The free-falling drop detector--a nover fluorescence detector for high-performance liquid chromatography. A novel fluorescence detector for high-performance liquid chromatography has been designed to minimize the instrumental limitations of conventional flow-through, fluorometers. This unit contributes less than 5 mul to the \"dead volume\" of the chromatographic system. Sensitivity comparable to that of existing flow-through fluorometers has been attained without optimizing instrumental parameters. Performance of the unit has been evaluated with reference samples containing saccharides typical of glycosphingolipids. Future improvements are discussed.", "PMID": 954192}
{"id": "pubmed23n0032_973", "title": "A propagation of error analysis of the enzyme activity expression. A model for determining the total system random error of a kinetic enzyme analyzer.", "content": "We present a total system error evaluation of random error, based on a propagation of error analysis of the expression for the calculation of enzyme activity. A simple expression is derived that contains terms for photometric error, timing uncertainty, temperature-control error, sample and reagent volume errors, and pathlength error. This error expression was developed in general to provide a simple means of evaluating the magnitude of random error in an analytical system and in particular to provide an error evaluation protocol for the assessment of the error components in a prototype Miniature Centrifugal Analyzer system. Individual system components of error are measured. These measured error components are combined in the error expressiion to predict performance. Enzyme activity measurements are made to correlate with the projected error data. In conclusion, it is demonstrated that this is one method for permitting the clinical chemist and the instrument manufacturer to establish reasonable error limits.", "contents": "A propagation of error analysis of the enzyme activity expression. A model for determining the total system random error of a kinetic enzyme analyzer. We present a total system error evaluation of random error, based on a propagation of error analysis of the expression for the calculation of enzyme activity. A simple expression is derived that contains terms for photometric error, timing uncertainty, temperature-control error, sample and reagent volume errors, and pathlength error. This error expression was developed in general to provide a simple means of evaluating the magnitude of random error in an analytical system and in particular to provide an error evaluation protocol for the assessment of the error components in a prototype Miniature Centrifugal Analyzer system. Individual system components of error are measured. These measured error components are combined in the error expressiion to predict performance. Enzyme activity measurements are made to correlate with the projected error data. In conclusion, it is demonstrated that this is one method for permitting the clinical chemist and the instrument manufacturer to establish reasonable error limits.", "PMID": 954193}
{"id": "pubmed23n0032_974", "title": "Modified nucleosides in urine: selective removal and analysis.", "content": "Because excretion of certain nucleosides increases in all types of cancer, detection and treatment of the disease would be enhanced by rapid, simple quantitation of such nucleotides. To facilitate these analyses in urine, we have prepared a specific adsorbent for nucleosides. The affinity adsorbent gel contains an immobilized phenylboronic acid group that specifically binds cis-diols, as in ribonucleosides, which can then be quantititatively recovered by elution with acetic acid. The advantage of this boronate gel over other preparations lies in its high capacity in the swollen state (0.26 mmol/ml), so that only small volumes (less than 1 ml) of gel are required for assays. Nucelosides containing a free diol group on the ribosyl group are retained at pH 8.8 in 0.25 mol/liter salt solution with a retention volume of 15 or larger, as compared with values of 1 to 2 for the free bases. Negatively charged species (not including the boronate complex) are eluted earlier than neutral or cationic species. Added nucleosides (neutral, cationic, or anionic) are quantitatively recovered. Because pseudouridine is eluted in a unique position, this property has been used to measure it in urine. The procedures used are chemically mild. For example, we have confirmed the observation of Fink and Adams [Arch. Biochem. Biophys. 126, 27 (1968)] that urine contains N1-methyladenosine and not N6-methyladenosine, the alkaline rearranged product.", "contents": "Modified nucleosides in urine: selective removal and analysis. Because excretion of certain nucleosides increases in all types of cancer, detection and treatment of the disease would be enhanced by rapid, simple quantitation of such nucleotides. To facilitate these analyses in urine, we have prepared a specific adsorbent for nucleosides. The affinity adsorbent gel contains an immobilized phenylboronic acid group that specifically binds cis-diols, as in ribonucleosides, which can then be quantititatively recovered by elution with acetic acid. The advantage of this boronate gel over other preparations lies in its high capacity in the swollen state (0.26 mmol/ml), so that only small volumes (less than 1 ml) of gel are required for assays. Nucelosides containing a free diol group on the ribosyl group are retained at pH 8.8 in 0.25 mol/liter salt solution with a retention volume of 15 or larger, as compared with values of 1 to 2 for the free bases. Negatively charged species (not including the boronate complex) are eluted earlier than neutral or cationic species. Added nucleosides (neutral, cationic, or anionic) are quantitatively recovered. Because pseudouridine is eluted in a unique position, this property has been used to measure it in urine. The procedures used are chemically mild. For example, we have confirmed the observation of Fink and Adams [Arch. Biochem. Biophys. 126, 27 (1968)] that urine contains N1-methyladenosine and not N6-methyladenosine, the alkaline rearranged product.", "PMID": 954194}
{"id": "pubmed23n0032_975", "title": "Instrumental sample-preparation technique for an automated immunohematology assay system.", "content": "In the course of the development of an automated immunohematology assay system, we have produced, in prototype form, an instrumental device capable of accepting an unmeasured quantity of whole, anticoagulated blood and deriving from it multiple fixed-volume aliquots of plasma and cells.", "contents": "Instrumental sample-preparation technique for an automated immunohematology assay system. In the course of the development of an automated immunohematology assay system, we have produced, in prototype form, an instrumental device capable of accepting an unmeasured quantity of whole, anticoagulated blood and deriving from it multiple fixed-volume aliquots of plasma and cells.", "PMID": 954195}
{"id": "pubmed23n0032_976", "title": "Multicomponent analysis in clinical chemistry by use of rapid scanning fluorescence spectroscopy.", "content": "To be useful in the clinical laboratory, multicomponent fluorescence analysis requires both the rapid measurement of the fluorescence intensity at a variety of excitation and emission wavelengths and the unambiguous reduction of the data by efficient algorithms. The Video Fluorometer, which exploits the multi-channel capability of a low-light-level television sensor to simultaneously acquire excitation and emission spectra, can meet the first requirement. For example, a complete set of emission and excitation spectra for perylene can be obtained in less than 2 s at concentrations of 10(-10) mol/liter. To meet the second need, we present two types of data-reduction strategies: (a)a least-squares fit to the data, with use of the spectra of previously determined compounds likely to be present; and (b)a determination of the eigenvalues and eigenvectors fo the fluorescence matrix, from which the number of components and the possible spectra of each can be estimated. Computer simulations of the least-squares fitting algorithms show that five strongly overlapping components can be determined in the presence of noise with an accuracy of better than 5%. Also, a fluorescent sample containing two species with very similar but unknown spectral properties can be resolved to obtain the spectrum of each.", "contents": "Multicomponent analysis in clinical chemistry by use of rapid scanning fluorescence spectroscopy. To be useful in the clinical laboratory, multicomponent fluorescence analysis requires both the rapid measurement of the fluorescence intensity at a variety of excitation and emission wavelengths and the unambiguous reduction of the data by efficient algorithms. The Video Fluorometer, which exploits the multi-channel capability of a low-light-level television sensor to simultaneously acquire excitation and emission spectra, can meet the first requirement. For example, a complete set of emission and excitation spectra for perylene can be obtained in less than 2 s at concentrations of 10(-10) mol/liter. To meet the second need, we present two types of data-reduction strategies: (a)a least-squares fit to the data, with use of the spectra of previously determined compounds likely to be present; and (b)a determination of the eigenvalues and eigenvectors fo the fluorescence matrix, from which the number of components and the possible spectra of each can be estimated. Computer simulations of the least-squares fitting algorithms show that five strongly overlapping components can be determined in the presence of noise with an accuracy of better than 5%. Also, a fluorescent sample containing two species with very similar but unknown spectral properties can be resolved to obtain the spectrum of each.", "PMID": 954196}
{"id": "pubmed23n0032_977", "title": "Centrifugal system for affinity chromatography with eluate monitoring.", "content": "A prototype centrifugal system has been developed that permits parallel photometric monitoring of eluate streams from a radial array of chromatographic columns. The modular rotor design consists of a discoidal center insert for eluent and sample apportionment, the chromatographic columns, and flow-through cuvetts-all mounted on an aluminum base plate. A common sample is introduced simultaneously to each column; a single eluent stream is used for all columns. The goal is to assay simultaneously for eight to 16 serum proteins separated by affinity chromatography from a single sample. The system is here exemplified by the use of immunosorbents (consisting of antibodies to human immunoglobulins covalently bound to alumina particles) to allow simultaneous determinations of IgG AND IgM from a single human serum sample in less than 16 min.", "contents": "Centrifugal system for affinity chromatography with eluate monitoring. A prototype centrifugal system has been developed that permits parallel photometric monitoring of eluate streams from a radial array of chromatographic columns. The modular rotor design consists of a discoidal center insert for eluent and sample apportionment, the chromatographic columns, and flow-through cuvetts-all mounted on an aluminum base plate. A common sample is introduced simultaneously to each column; a single eluent stream is used for all columns. The goal is to assay simultaneously for eight to 16 serum proteins separated by affinity chromatography from a single sample. The system is here exemplified by the use of immunosorbents (consisting of antibodies to human immunoglobulins covalently bound to alumina particles) to allow simultaneous determinations of IgG AND IgM from a single human serum sample in less than 16 min.", "PMID": 954197}
{"id": "pubmed23n0032_978", "title": "Automated approach to radioimmunoassays of somatotropin (human growth hormone) and insulin.", "content": "The adaptation of human somatotropin and insulin assays to the automated Centria radioimmunoassay system [Clin. Chem. 21, 1305 (1975)] is reported. For the somatotropin assay, reaction conditions include a borate/bovine serum albumin buffer (pH 8.4) AND 20-H Incubation at 4 degrees C. Assay results for clinical samples compared favorably (correlation coefficient = 0.930) with values obtained from a reference laboratory. The means determined for 92 patients' samples were 4.3 mug/liter (reference laboratory) and 5.1 mug/liter (Centria). Intra- and inter-run precision ranged from 3.2 to 15.9%. For the insulin assay, a phosphate/bovine serum albumin buffer (pH 7.4) is used, with a 20-h incubation at 4 degrees C. Previously analyzed insulin samples from a reference laboratory were determined by the Centria analyzer with excellent correlation (r = 0.965). Means for patients' samples were 43.0 milli (USP) units of insulin per liter (reference laboratory) and 47.5 milliunits of insulin per liter (Centria). In both assays an anionicexchange gel is used in the separation step. The criterion of parallelism, an indication of the validity of a radioimmunoassay, was satisfied in both assays. The Centria radioimmunoassay system offers the advantage of automating all the critical steps of these radioimmunoassays.", "contents": "Automated approach to radioimmunoassays of somatotropin (human growth hormone) and insulin. The adaptation of human somatotropin and insulin assays to the automated Centria radioimmunoassay system [Clin. Chem. 21, 1305 (1975)] is reported. For the somatotropin assay, reaction conditions include a borate/bovine serum albumin buffer (pH 8.4) AND 20-H Incubation at 4 degrees C. Assay results for clinical samples compared favorably (correlation coefficient = 0.930) with values obtained from a reference laboratory. The means determined for 92 patients' samples were 4.3 mug/liter (reference laboratory) and 5.1 mug/liter (Centria). Intra- and inter-run precision ranged from 3.2 to 15.9%. For the insulin assay, a phosphate/bovine serum albumin buffer (pH 7.4) is used, with a 20-h incubation at 4 degrees C. Previously analyzed insulin samples from a reference laboratory were determined by the Centria analyzer with excellent correlation (r = 0.965). Means for patients' samples were 43.0 milli (USP) units of insulin per liter (reference laboratory) and 47.5 milliunits of insulin per liter (Centria). In both assays an anionicexchange gel is used in the separation step. The criterion of parallelism, an indication of the validity of a radioimmunoassay, was satisfied in both assays. The Centria radioimmunoassay system offers the advantage of automating all the critical steps of these radioimmunoassays.", "PMID": 954198}
{"id": "pubmed23n0032_979", "title": "Profiles of ultraviolet-absorbing components of urine from autistic children, as obtained by high-resolution ion-exchange chromatography.", "content": "Urines from 19 autistic children (3 female, 16 male) were analyzed by high-resolution, high-pressure anion-exchange chromatography. The results showed abnormalities in the excretion of hippuric acid, 4-hydroxyhippuric acid, and N1-methyl-2-pyridone-5-carboxamide, the end product of the nicotinic acid pathway. Considerations as the metabolic origin on the 4-hydroxyhippuric acid are discussed and related to the possibility of bacterial action and malabsorption.", "contents": "Profiles of ultraviolet-absorbing components of urine from autistic children, as obtained by high-resolution ion-exchange chromatography. Urines from 19 autistic children (3 female, 16 male) were analyzed by high-resolution, high-pressure anion-exchange chromatography. The results showed abnormalities in the excretion of hippuric acid, 4-hydroxyhippuric acid, and N1-methyl-2-pyridone-5-carboxamide, the end product of the nicotinic acid pathway. Considerations as the metabolic origin on the 4-hydroxyhippuric acid are discussed and related to the possibility of bacterial action and malabsorption.", "PMID": 954199}
{"id": "pubmed23n0032_980", "title": "A simple and rapid determination of the lecithin/sphingomyelin (LS) ratio in amniotic fluids and in pharyngeal aspirates of newborn infants.", "content": "A simple, rapid method for the routine determination of the lecithin/sphingomyelin (L/S) ratio in amniotic fluid and/or in pharyngeal aspirate of the newborn has been developed. In 33 samples of amniotic fluid at various gestational ages, an L/S ratio corresponding to the fetal age was found: in 16 samples of biochemically immature amniotic fluid (gestation age less than 34 weeks) the mean of the ratio was 1.26 and in 17 samples of mature amniotic fluid (gestation age greater than 35 weeks) the mean value was 3.02. Pharyngeal aspirates of 55 non-selected newborn were examined by the same method. The L/S ratio of 39 infants (gestation age greater than 38 weeks) gave a mean value of 7.3 and that of 16 premature infants (gestation age less than 37 weeks) a mean value of 5.5. In none of these cases did RDS develop after birth. The results suggest that the method is useful for the determination in both amniotic fluid and pharyngeal aspirate.", "contents": "A simple and rapid determination of the lecithin/sphingomyelin (LS) ratio in amniotic fluids and in pharyngeal aspirates of newborn infants. A simple, rapid method for the routine determination of the lecithin/sphingomyelin (L/S) ratio in amniotic fluid and/or in pharyngeal aspirate of the newborn has been developed. In 33 samples of amniotic fluid at various gestational ages, an L/S ratio corresponding to the fetal age was found: in 16 samples of biochemically immature amniotic fluid (gestation age less than 34 weeks) the mean of the ratio was 1.26 and in 17 samples of mature amniotic fluid (gestation age greater than 35 weeks) the mean value was 3.02. Pharyngeal aspirates of 55 non-selected newborn were examined by the same method. The L/S ratio of 39 infants (gestation age greater than 38 weeks) gave a mean value of 7.3 and that of 16 premature infants (gestation age less than 37 weeks) a mean value of 5.5. In none of these cases did RDS develop after birth. The results suggest that the method is useful for the determination in both amniotic fluid and pharyngeal aspirate.", "PMID": 954209}
{"id": "pubmed23n0032_981", "title": "A study of the Bratton and Marshall hydrolysis procedure utilizing high performance liquid chromatography.", "content": "Most analytical methods for conjugated aromatic amines require hydrolysis to free the amine group. The rate of conversion of N-acetyl-p-aminohippuric acid to its deacetylated and deglycinated derivatives is directly related to the hydrolysis reaction time and the concentration of hydrochloric acid used in the reaction. Variations in methodology involving either of these hydrolysis parameters produced artifacts which were not detectable by the standard colorimetric procedures. High performance liquid chromatography offers an improved tool for detecting and quantitating the changes produced during hydrolysis.", "contents": "A study of the Bratton and Marshall hydrolysis procedure utilizing high performance liquid chromatography. Most analytical methods for conjugated aromatic amines require hydrolysis to free the amine group. The rate of conversion of N-acetyl-p-aminohippuric acid to its deacetylated and deglycinated derivatives is directly related to the hydrolysis reaction time and the concentration of hydrochloric acid used in the reaction. Variations in methodology involving either of these hydrolysis parameters produced artifacts which were not detectable by the standard colorimetric procedures. High performance liquid chromatography offers an improved tool for detecting and quantitating the changes produced during hydrolysis.", "PMID": 954210}
{"id": "pubmed23n0032_982", "title": "Creatine kinase activity in serum and uric acid solution.", "content": "Skeletal muscle creatine kinases from man and rabbit, activated to measured specific activities by thiol agents, were inactivated in human serum and in uric acid solution, and the extent of their subsequent reactivation by dithiothreitol was determined. Both enzymes were completely reactivated from uric acid solution but were only 60-70% reactivated from serum. No inactivation occurred within 14 days if serum samples contained dithiothreitol (0.01 M) and were stored at -17 degrees C.", "contents": "Creatine kinase activity in serum and uric acid solution. Skeletal muscle creatine kinases from man and rabbit, activated to measured specific activities by thiol agents, were inactivated in human serum and in uric acid solution, and the extent of their subsequent reactivation by dithiothreitol was determined. Both enzymes were completely reactivated from uric acid solution but were only 60-70% reactivated from serum. No inactivation occurred within 14 days if serum samples contained dithiothreitol (0.01 M) and were stored at -17 degrees C.", "PMID": 954211}
{"id": "pubmed23n0032_983", "title": "Studies of intracellular distribution of acid phosphatase 5 in the spleen cells of leukemic reticuloendotheliosis by isopycnic gradient centrifugation.", "content": "A fresh spleen sample obtained from a patient with leukemic reticuloendotheliosis was homogenized and subjected to centrifugation on a sucrose density gradient. A major portion of acid phosphatase band 5 was observed in the lysosome, confirming that the elevated phosphatase activity in the neoplastic spleen is a lysosomal enzyme. However, a significant amount of brand 5 was also observed in the microsome. The microsomal and lysosomal enzymes have different affinity to CM-cellulose. The relationship between lysosomal and microsomal enzymes has not been established.", "contents": "Studies of intracellular distribution of acid phosphatase 5 in the spleen cells of leukemic reticuloendotheliosis by isopycnic gradient centrifugation. A fresh spleen sample obtained from a patient with leukemic reticuloendotheliosis was homogenized and subjected to centrifugation on a sucrose density gradient. A major portion of acid phosphatase band 5 was observed in the lysosome, confirming that the elevated phosphatase activity in the neoplastic spleen is a lysosomal enzyme. However, a significant amount of brand 5 was also observed in the microsome. The microsomal and lysosomal enzymes have different affinity to CM-cellulose. The relationship between lysosomal and microsomal enzymes has not been established.", "PMID": 954212}
{"id": "pubmed23n0032_984", "title": "A method for performing a calcium balance study in man and the interpretation of results.", "content": "Methods for the measurement of calcium in the diet, urine and faeces for the performance of a calcium balance study was described, along with experiments on analytical procedures including recovery values. A means of calculating the inherent \"technical error\" in such a balance is given, and a method for determining the significance of any change in a patient's balance is described. These are illustrated by worked examples of data from a patient suffering from Paget's disease and one with osteoporosis before and after treatment with calcitonin.", "contents": "A method for performing a calcium balance study in man and the interpretation of results. Methods for the measurement of calcium in the diet, urine and faeces for the performance of a calcium balance study was described, along with experiments on analytical procedures including recovery values. A means of calculating the inherent \"technical error\" in such a balance is given, and a method for determining the significance of any change in a patient's balance is described. These are illustrated by worked examples of data from a patient suffering from Paget's disease and one with osteoporosis before and after treatment with calcitonin.", "PMID": 954213}
{"id": "pubmed23n0032_985", "title": "The mass spectrometric identification of dipeptides in the urine of a patient suffering from chronic skin ulceration and oedema.", "content": "The identification of a complex ninhydrin positive mixture present in the urine of a child suffering from chronic skin ulceration and oedema by direct chemical ionisation mass spectrometric analysis is described. The compounds were shown to be dipeptides, of which glycylproline was the major constituent. At least 15 dipeptides were identified in the urine, most of which contained proline or hydroxyproline in the carboxy terminal position. The results suggested that the patient suffered from a defect in collagen metabolism. This hypothesis was subsequently confirmed by a grossly diminished level of prolidase in cultured fibroblasts and erythrocytes.", "contents": "The mass spectrometric identification of dipeptides in the urine of a patient suffering from chronic skin ulceration and oedema. The identification of a complex ninhydrin positive mixture present in the urine of a child suffering from chronic skin ulceration and oedema by direct chemical ionisation mass spectrometric analysis is described. The compounds were shown to be dipeptides, of which glycylproline was the major constituent. At least 15 dipeptides were identified in the urine, most of which contained proline or hydroxyproline in the carboxy terminal position. The results suggested that the patient suffered from a defect in collagen metabolism. This hypothesis was subsequently confirmed by a grossly diminished level of prolidase in cultured fibroblasts and erythrocytes.", "PMID": 954214}
{"id": "pubmed23n0032_986", "title": "Relationship of plasma uric acid to plasma lipids and lipoproteins in subjects with peripheral vascular disease.", "content": "Hyperuricaemia and hyperlipidaemia (elevated fasting plasma cholesterol or triglycerides) were frequently found in 219 males and 63 females with peripheral vascular disease (PVD). The subjects were divided into sexes and the uric acid, cholesterol and triglyceride concentration adjusted for the effects of age and obesity by multiple regression analysis. Followig this no significant relationship was found between uric acid and cholesterol or triglyceride. When the males with PVD were divided into lipoprotein types it was found that those who were normo-lipoproteinaemics or who had type IV hyperlipoproteinaemia (HLP) had a significantly higher mean uric acid level. The other types had a mean uric acid concentration similar to that found in 25 healthy normolipoproteinaemic males. The discrepancy between this result and the lack of correlation between uric acid and triglyceride noted above is presumably due to the complex effects of age and obesity. In the females no significant increase in the mean uric acid concentration was found in any of the lipoprotein groups.", "contents": "Relationship of plasma uric acid to plasma lipids and lipoproteins in subjects with peripheral vascular disease. Hyperuricaemia and hyperlipidaemia (elevated fasting plasma cholesterol or triglycerides) were frequently found in 219 males and 63 females with peripheral vascular disease (PVD). The subjects were divided into sexes and the uric acid, cholesterol and triglyceride concentration adjusted for the effects of age and obesity by multiple regression analysis. Followig this no significant relationship was found between uric acid and cholesterol or triglyceride. When the males with PVD were divided into lipoprotein types it was found that those who were normo-lipoproteinaemics or who had type IV hyperlipoproteinaemia (HLP) had a significantly higher mean uric acid level. The other types had a mean uric acid concentration similar to that found in 25 healthy normolipoproteinaemic males. The discrepancy between this result and the lack of correlation between uric acid and triglyceride noted above is presumably due to the complex effects of age and obesity. In the females no significant increase in the mean uric acid concentration was found in any of the lipoprotein groups.", "PMID": 954215}
{"id": "pubmed23n0032_987", "title": "The assessment of 125I treatment of thyrotoxicosis.", "content": "257 patients have been reviewed 1-5 years (mean 3 years 2 months) after receiving one of five dose regimes of 125I for thyrotoxicosis. The cumulative incidence of hypothyroidism was 34% and of persistent thyrotoxicosis 17%. The group receiving doses between 351 and 500 muCi/g had the highest proportion of euthyroid patients (65%) with the lowest requirement for repeat therapy (46%). In the euthyroid patients, increasing dose of 125I was associated with progressive decline in mean thyroxine (T4) level and free thyroxine index (FTI) within the respective normal ranges, and increase in mean thyroid stimulating hormone (TSH) level to above the normal range. Euthyroid patients with elevated TSH levels had significantly lower T4 and FTI values compared with those with normal TSH, and showed a 3-4-fold increased rate of development of hypothyroidism over 1 year. Euthyroid patients with elevated T3 levels remained euthyroid during the subsequent year and mean T3 levels declined significantly, suggesting that abnormally elevated T3 levels after 125I do not generally indicate impending relapse of thyrotoxicosis. It is concluded that the potential admantages of 125I therapy for thyrotoxicosis in reducing the incidence of hypothyroidism have not been realized in practice.", "contents": "The assessment of 125I treatment of thyrotoxicosis. 257 patients have been reviewed 1-5 years (mean 3 years 2 months) after receiving one of five dose regimes of 125I for thyrotoxicosis. The cumulative incidence of hypothyroidism was 34% and of persistent thyrotoxicosis 17%. The group receiving doses between 351 and 500 muCi/g had the highest proportion of euthyroid patients (65%) with the lowest requirement for repeat therapy (46%). In the euthyroid patients, increasing dose of 125I was associated with progressive decline in mean thyroxine (T4) level and free thyroxine index (FTI) within the respective normal ranges, and increase in mean thyroid stimulating hormone (TSH) level to above the normal range. Euthyroid patients with elevated TSH levels had significantly lower T4 and FTI values compared with those with normal TSH, and showed a 3-4-fold increased rate of development of hypothyroidism over 1 year. Euthyroid patients with elevated T3 levels remained euthyroid during the subsequent year and mean T3 levels declined significantly, suggesting that abnormally elevated T3 levels after 125I do not generally indicate impending relapse of thyrotoxicosis. It is concluded that the potential admantages of 125I therapy for thyrotoxicosis in reducing the incidence of hypothyroidism have not been realized in practice.", "PMID": 954217}
{"id": "pubmed23n0032_988", "title": "Response of plasma testosterone, urinary 17-oxosteroids, oestrogens, and androsterone plus aetiocholanolone to human chorionic gonadotrophin in dexamethasone-suppressed men.", "content": "The administration of human chorionic gonadotrophin (HCG) to dexamethasone-suppressed men caused parallel changes in the concentration of plasma testosterone and in the urinary output of androsterone+aetiocholanolone, total 17-oxosteroids and oestrogens. Discrepant results occurred in only four of the thirty-seven men tested. With these exceptions, the response to HCG could by followed as well by measuring androsterone+aetiocholanolone, 17-oxosteroid or oestrogen excretion rates as by following plasma testosterone levels. The most sensitive index of response was the rate of appearance of oestrogens in urine, and the next that of androsterone+aetiocholanolone.", "contents": "Response of plasma testosterone, urinary 17-oxosteroids, oestrogens, and androsterone plus aetiocholanolone to human chorionic gonadotrophin in dexamethasone-suppressed men. The administration of human chorionic gonadotrophin (HCG) to dexamethasone-suppressed men caused parallel changes in the concentration of plasma testosterone and in the urinary output of androsterone+aetiocholanolone, total 17-oxosteroids and oestrogens. Discrepant results occurred in only four of the thirty-seven men tested. With these exceptions, the response to HCG could by followed as well by measuring androsterone+aetiocholanolone, 17-oxosteroid or oestrogen excretion rates as by following plasma testosterone levels. The most sensitive index of response was the rate of appearance of oestrogens in urine, and the next that of androsterone+aetiocholanolone.", "PMID": 954218}
{"id": "pubmed23n0032_989", "title": "Galactrorrhoea, hyperprolactinaemia and pituitary tumours in the female.", "content": "A series of sixty-two women with pituitary tumours, but having neither acromegaly nor Cushing's disease, has been analysed with respect to mode of presentation. Thirty-six (58%) presented with amenorrhoea and galactorrhoea and a further fifteen (24%) presented with amenorrhoea only. Pretreatment prolactin levels were available in thirty-four of these patients and in twenty-seven (79%) hyperprolactinaemia was found, this occurring in the absence of a history of galactorrhoea in four out of ten cases. In addition, among a series of twenty-five unselected women with unexplained galactorrhoea, we have found twelve pituitary tumours, amenorrhoea being a feature of all twelve cases. We conclude that the incidence of hyperprolactinaemia in women with apparently functionless pituitary tumours is much higher than previously estimated and suggest that serum prolactin should be assayed in all patients with such tumours even in the absence of a history of galactorrhoea. All patients with unexplained galactorrhoea should have careful radiological investigation for a possible pituitary tumour.", "contents": "Galactrorrhoea, hyperprolactinaemia and pituitary tumours in the female. A series of sixty-two women with pituitary tumours, but having neither acromegaly nor Cushing's disease, has been analysed with respect to mode of presentation. Thirty-six (58%) presented with amenorrhoea and galactorrhoea and a further fifteen (24%) presented with amenorrhoea only. Pretreatment prolactin levels were available in thirty-four of these patients and in twenty-seven (79%) hyperprolactinaemia was found, this occurring in the absence of a history of galactorrhoea in four out of ten cases. In addition, among a series of twenty-five unselected women with unexplained galactorrhoea, we have found twelve pituitary tumours, amenorrhoea being a feature of all twelve cases. We conclude that the incidence of hyperprolactinaemia in women with apparently functionless pituitary tumours is much higher than previously estimated and suggest that serum prolactin should be assayed in all patients with such tumours even in the absence of a history of galactorrhoea. All patients with unexplained galactorrhoea should have careful radiological investigation for a possible pituitary tumour.", "PMID": 954219}
{"id": "pubmed23n0032_990", "title": "Effect of acute and chronic neuroleptic therapy on serum prolactin levels in men and women of different age groups.", "content": "A single fasting level of serum prolactin was measured in each of sixty control subjects and eighty-three psychiatric patients of both sexes who had been on neuroleptic therapy for 2-4 weeks (acute treatment) or at least 5 years (chronic treatment) and who were aged either 17-45 or 48-85 years. All groups of patients had significantly higher mean prolactin levels than controls. Gender, age group of women, and exposure to acute or chronic treatment were significant variables determining the magnitude of neuroleptic-induced elevation of prolactin. In some of the groups, dose, duration of chronic therapy, and concomitant administration of anticholinergic drugs also influenced prolactin levels. Whereas all acutely treated women had prolactin values above the control range, one out of twelve (8-3%) of the women aged 17-45 years and six out of fourteen (42-9%) of the women aged 48-85 years who were under chronic treatment had normal values. Normal prolactin levels were also found in five out of sixteen (31-2%) of the acutely treated and nine out of twenty-four (37-5%) of the chronically treated men aged 17-85 years.", "contents": "Effect of acute and chronic neuroleptic therapy on serum prolactin levels in men and women of different age groups. A single fasting level of serum prolactin was measured in each of sixty control subjects and eighty-three psychiatric patients of both sexes who had been on neuroleptic therapy for 2-4 weeks (acute treatment) or at least 5 years (chronic treatment) and who were aged either 17-45 or 48-85 years. All groups of patients had significantly higher mean prolactin levels than controls. Gender, age group of women, and exposure to acute or chronic treatment were significant variables determining the magnitude of neuroleptic-induced elevation of prolactin. In some of the groups, dose, duration of chronic therapy, and concomitant administration of anticholinergic drugs also influenced prolactin levels. Whereas all acutely treated women had prolactin values above the control range, one out of twelve (8-3%) of the women aged 17-45 years and six out of fourteen (42-9%) of the women aged 48-85 years who were under chronic treatment had normal values. Normal prolactin levels were also found in five out of sixteen (31-2%) of the acutely treated and nine out of twenty-four (37-5%) of the chronically treated men aged 17-85 years.", "PMID": 954220}
{"id": "pubmed23n0032_991", "title": "Clinical use of glycine intravenous load for diagnosis of growth hormone deficiency.", "content": "Intravenous glycine injection (250 mg/kg of body weight) resulted in growth hormone release in normal children but not in those with growth hormone deficiency diagnosed by insulin-induced hypoglycaemia. In the latter significantly higher peak concentrations of serum alpha-amino nitrogen were also found. False negative responses to glycine (no GH release) were observed in two patients of short stature but normal pituitary function. In them the peak levels of serum alpha-amino nitrogen were lower than in those with hypopituitarism. We propose the clinical use of glycine as an inexpensive and innocuous procedure for the detection of GH deficiency in children. A post-glycine GH peak greater than 10-0 mu/l seems to be a good index of an intact GH reserve.", "contents": "Clinical use of glycine intravenous load for diagnosis of growth hormone deficiency. Intravenous glycine injection (250 mg/kg of body weight) resulted in growth hormone release in normal children but not in those with growth hormone deficiency diagnosed by insulin-induced hypoglycaemia. In the latter significantly higher peak concentrations of serum alpha-amino nitrogen were also found. False negative responses to glycine (no GH release) were observed in two patients of short stature but normal pituitary function. In them the peak levels of serum alpha-amino nitrogen were lower than in those with hypopituitarism. We propose the clinical use of glycine as an inexpensive and innocuous procedure for the detection of GH deficiency in children. A post-glycine GH peak greater than 10-0 mu/l seems to be a good index of an intact GH reserve.", "PMID": 954221}
{"id": "pubmed23n0032_992", "title": "The effect of varying doses of cerebral irradiation on growth hormone production in childhood.", "content": "The radiation dose, delivered to the hypothalamic-pituitary region, has been calculated in thirty-nine children irradiated for brain tumours and in seventeen children who had received prophylactic cranial irradiation for acute leukaemia. All subjects had an insulin tolerance test at least 2 years after their radiotherapy. There is a significant inverse correlation between radiation dose and peak GH response. Thirty-seven of the fifty-six patients showed an impaired GH response and thirty-six of these received more than 2900 rads. Only five patients who received such a dose showed normal GH responses and four of these were aged over 13 years when treated. In conclusion, cerebral irradiation is a potent cause of GH deficiency in children.", "contents": "The effect of varying doses of cerebral irradiation on growth hormone production in childhood. The radiation dose, delivered to the hypothalamic-pituitary region, has been calculated in thirty-nine children irradiated for brain tumours and in seventeen children who had received prophylactic cranial irradiation for acute leukaemia. All subjects had an insulin tolerance test at least 2 years after their radiotherapy. There is a significant inverse correlation between radiation dose and peak GH response. Thirty-seven of the fifty-six patients showed an impaired GH response and thirty-six of these received more than 2900 rads. Only five patients who received such a dose showed normal GH responses and four of these were aged over 13 years when treated. In conclusion, cerebral irradiation is a potent cause of GH deficiency in children.", "PMID": 954222}
{"id": "pubmed23n0032_993", "title": "Recurrent hormone dependent chorea: effects of oestrogens and progestogens.", "content": "A case of hemi-chorea is described in a young woman, initially following administration of an oral contraceptive pill and recurring in a subsequent early pregnancy. The chorea ceased with the removal of the aetiological factor on each occasion. Subsequent challenge with a combined oestrogen/progestogen pill resulted in recurrence of the chorea, but the patient has been successfully maintained on a progestogen without any further symptoms. Thus it would appear that oestrogen component was the precipitating factor for the chorea.", "contents": "Recurrent hormone dependent chorea: effects of oestrogens and progestogens. A case of hemi-chorea is described in a young woman, initially following administration of an oral contraceptive pill and recurring in a subsequent early pregnancy. The chorea ceased with the removal of the aetiological factor on each occasion. Subsequent challenge with a combined oestrogen/progestogen pill resulted in recurrence of the chorea, but the patient has been successfully maintained on a progestogen without any further symptoms. Thus it would appear that oestrogen component was the precipitating factor for the chorea.", "PMID": 954223}
{"id": "pubmed23n0032_994", "title": "Quantitation of the accelerating effect of metyrapone on cortisol metabolism.", "content": "Quantitation of the accelerating effect of metyrapone on cortisol metabolism has been made by determination of the metabolic clearance rate (MCR) of exogenous cortisol during a metyrapone load. Six adrenalectomized patients were studied. The slope of cortisol concentrations in plasma was determined after intravenous administration of 0-3 mg cortisol/kg b.w. with or without metyrapone 17-5 mg/kg b.w./h. In all six patients studied, the MCR of cortisol increased during metyrapone load from an average of 12-3 +/- 5-0 (SD) l/h to 29-6 +/- 15-7 (SD) l/h or corrected for body weight 3-0 +/- 1-1 (SD) ml/kg b.w./min to 7-1 +/- 2-5 (SD) ml/kg b.w./min. The discrepancy discovered in clinical practice between the relatively small rise in plasma total corticosteroid concentration and the greater increase in urinary excretion of corticosteroid metabolites during the metyrapone test may be explained by the findings in this study.", "contents": "Quantitation of the accelerating effect of metyrapone on cortisol metabolism. Quantitation of the accelerating effect of metyrapone on cortisol metabolism has been made by determination of the metabolic clearance rate (MCR) of exogenous cortisol during a metyrapone load. Six adrenalectomized patients were studied. The slope of cortisol concentrations in plasma was determined after intravenous administration of 0-3 mg cortisol/kg b.w. with or without metyrapone 17-5 mg/kg b.w./h. In all six patients studied, the MCR of cortisol increased during metyrapone load from an average of 12-3 +/- 5-0 (SD) l/h to 29-6 +/- 15-7 (SD) l/h or corrected for body weight 3-0 +/- 1-1 (SD) ml/kg b.w./min to 7-1 +/- 2-5 (SD) ml/kg b.w./min. The discrepancy discovered in clinical practice between the relatively small rise in plasma total corticosteroid concentration and the greater increase in urinary excretion of corticosteroid metabolites during the metyrapone test may be explained by the findings in this study.", "PMID": 954224}
{"id": "pubmed23n0032_995", "title": "The Wolf-Hirschhorn (4p-) syndrome.", "content": "In a review of 43 cases, the phenotypic spectrum of the Wolf-Hirschhorn syndrome is analyzed and the frequency of clinical anomalies is tabulated. The characteristic features are intrauterine growth retardation, severe psychomotor retardation, typical facies, and various major and minor congenital anomalies suggestive of a midline fusion defect. Diagnosis is established by karyotyping- deletion of the short arm of chromosome No. 4. All cases so far reported are de novo occurrences with no sibling involvement and normal parents. Prognosis is poor, with death in the first 2 years of life in 34% of cases, usually due to cardiac decompensation or infection. Psyhomotor retardation is profound, so that heroic medical efforts probably need to be reconsidered.", "contents": "The Wolf-Hirschhorn (4p-) syndrome. In a review of 43 cases, the phenotypic spectrum of the Wolf-Hirschhorn syndrome is analyzed and the frequency of clinical anomalies is tabulated. The characteristic features are intrauterine growth retardation, severe psychomotor retardation, typical facies, and various major and minor congenital anomalies suggestive of a midline fusion defect. Diagnosis is established by karyotyping- deletion of the short arm of chromosome No. 4. All cases so far reported are de novo occurrences with no sibling involvement and normal parents. Prognosis is poor, with death in the first 2 years of life in 34% of cases, usually due to cardiac decompensation or infection. Psyhomotor retardation is profound, so that heroic medical efforts probably need to be reconsidered.", "PMID": 954225}
{"id": "pubmed23n0032_996", "title": "Human chromosome polymorphism and congenital malformations.", "content": "Several authors have suggested that heterochromatin polymorphism influences the origin and/or development of different malformations. In this investigation special consideration was given to the A1qh + variant. Several families with this variant are reported in which the incidence of otherwise rare malformations is surprisingly high. The possibility that the A1qh+ varient or, more probably, interactions between all types of heterochromatin polymorphism are of pathogenetic significance is considered.", "contents": "Human chromosome polymorphism and congenital malformations. Several authors have suggested that heterochromatin polymorphism influences the origin and/or development of different malformations. In this investigation special consideration was given to the A1qh + variant. Several families with this variant are reported in which the incidence of otherwise rare malformations is surprisingly high. The possibility that the A1qh+ varient or, more probably, interactions between all types of heterochromatin polymorphism are of pathogenetic significance is considered.", "PMID": 954226}
{"id": "pubmed23n0032_997", "title": "Altered isoenzyme patterns of liver alpha-L-fucosidase in cystic fibrosis.", "content": "The isoenzyme pattern of alpha-L-fucosidase was studied by isoelectric focusing in livers from seven patients with cystic fibrosis and in normal and pathological (GM1-gangliosidosis, Type II and Sanfilippo disease) controls. The controls had very reproducible patterns consisting of seven isoenzymes of alpha-L-fucosidase with the most neutral from (I) representing a small proportion of the total activity. All seven of the cystic fibrosis livers had altered alpha-L-fucosidase isoenzyme patterns. The chemical relationship of the seven isoenzymes of normal liver alpha-L-fucosidase was investigated using neuramindase. The five most acidic forms of alpha-L-fucosidase appear to be related to the most neutral form by sialic acid residues. Since the isoenzymes of liver alpha-L-fucosidase appear to be related by sialic acid residues, it is possible that the altered alpha-L-fucosidase isoenzyme patterns found in cystic fibrosis livers may result from aberrant sialylation.", "contents": "Altered isoenzyme patterns of liver alpha-L-fucosidase in cystic fibrosis. The isoenzyme pattern of alpha-L-fucosidase was studied by isoelectric focusing in livers from seven patients with cystic fibrosis and in normal and pathological (GM1-gangliosidosis, Type II and Sanfilippo disease) controls. The controls had very reproducible patterns consisting of seven isoenzymes of alpha-L-fucosidase with the most neutral from (I) representing a small proportion of the total activity. All seven of the cystic fibrosis livers had altered alpha-L-fucosidase isoenzyme patterns. The chemical relationship of the seven isoenzymes of normal liver alpha-L-fucosidase was investigated using neuramindase. The five most acidic forms of alpha-L-fucosidase appear to be related to the most neutral form by sialic acid residues. Since the isoenzymes of liver alpha-L-fucosidase appear to be related by sialic acid residues, it is possible that the altered alpha-L-fucosidase isoenzyme patterns found in cystic fibrosis livers may result from aberrant sialylation.", "PMID": 954227}
{"id": "pubmed23n0032_998", "title": "A man with presumptive Y/Y translocation, observed in a forensic psychiatric department.", "content": "A man charged with a sexual offence was found to have complex karyotype anomaly, including a Y-chromosome of aberrant structure. Other features were: very small stature, skeletal deformities and obscure neurological defects. There were no gross psychiatric symptoms, nor was there mental retardation. Q- and G-banding and photometric scanning of the chromosomes of the propositus and his three healthy brothers indicated that the aberrant Y-chromosome probably arose from a reciprocal translocation, which may be written: t(Y: Y) (qter leads to p11: : q11 leads to qter). Fibroblast cultures differed from the lymphocytes: in the former the majority of the cells lacked the abnormal Y-chromosome, their karyotype being 45,X0.", "contents": "A man with presumptive Y/Y translocation, observed in a forensic psychiatric department. A man charged with a sexual offence was found to have complex karyotype anomaly, including a Y-chromosome of aberrant structure. Other features were: very small stature, skeletal deformities and obscure neurological defects. There were no gross psychiatric symptoms, nor was there mental retardation. Q- and G-banding and photometric scanning of the chromosomes of the propositus and his three healthy brothers indicated that the aberrant Y-chromosome probably arose from a reciprocal translocation, which may be written: t(Y: Y) (qter leads to p11: : q11 leads to qter). Fibroblast cultures differed from the lymphocytes: in the former the majority of the cells lacked the abnormal Y-chromosome, their karyotype being 45,X0.", "PMID": 954229}
{"id": "pubmed23n0032_999", "title": "Familial short atrioventricular conduction time (P-R interval).", "content": "Five sibs with related parents are presented. All the sibs have short atrioventricular conduction time (P-R interval). The short P-R interval is probably an inherited feature in the family.", "contents": "Familial short atrioventricular conduction time (P-R interval). Five sibs with related parents are presented. All the sibs have short atrioventricular conduction time (P-R interval). The short P-R interval is probably an inherited feature in the family.", "PMID": 954230}
{"id": "pubmed23n0032_1000", "title": "Genetic lipoprotein variation and lipid levels in man.", "content": "Analyses of 12 population samples have revealed that the total serum cholesterol level is significantly higher in Lp(a +) than in Lp(a -) individuals. The difference is more pronounced in middle-aged and other people than in young persons, and the difference between Lp(a +) and Lp(a -) individuals is larger when LDL cholesterol rather than total serum cholesterol is considered. Lp(a -) individuals have a significantly higher triglyceride level than Lp(a +) individuals. The Lp(a) variation is genetically controlled. Thus, the present study has revealed a small but significant effect of a single, autosomal locus on lipids implicated in atherosclerosis. An association between the phenotype Lp(a +) and coronary heart disease has been demonstrated previously.", "contents": "Genetic lipoprotein variation and lipid levels in man. Analyses of 12 population samples have revealed that the total serum cholesterol level is significantly higher in Lp(a +) than in Lp(a -) individuals. The difference is more pronounced in middle-aged and other people than in young persons, and the difference between Lp(a +) and Lp(a -) individuals is larger when LDL cholesterol rather than total serum cholesterol is considered. Lp(a -) individuals have a significantly higher triglyceride level than Lp(a +) individuals. The Lp(a) variation is genetically controlled. Thus, the present study has revealed a small but significant effect of a single, autosomal locus on lipids implicated in atherosclerosis. An association between the phenotype Lp(a +) and coronary heart disease has been demonstrated previously.", "PMID": 954231}
{"id": "pubmed23n0032_1001", "title": "Detection of circulating antigen-antibody complexes by their inhibitory effect on the agglutination of IgG-coated particles by rheumatioid factor of Clq.", "content": "The agglutination of Ig-coated particles by human RF or Clq can be inhibited by Ig aggregates or AgAb complexes. The effect of Ig class was studied by means of agarose-linked human monoclonal Igs. RF was inhibited by all subclasses of IgG and IgA but not by IgM, whereas Clq reacted with IgM, IgG3 and IgG1. Heat-aggregated IgG3 was fractionated by gel-filtration on Ultrogel. Inhibition was restricted to certain fractions of aggregates, viz (IgG3) approximately 7 and (IgG3) approximately 21 for RF, and (IgG3) approximately 10, (IgG3) approximately 14 and (IgG3) approximately 27 for Clq. In a precipitin curve experiment, it was found that RF was inhibited by soluble complexes over an extended range of AgAb ratios, the inactivation of Clq being limited to complexes with 2-5 times antigen excess. Inhibiting factors were found in patients with various diseases and, at low titres, in 22% of healthy people. In 27% of patients' sera, the inhibitors were demonstrable by Clq only after removal of endogenous RF by adsorption on insolubilized IgG. In several patients endogenous agglutinating activity and direct inhibitory activity tended to alternate during the course of the disease. Sera from various patients were also filtrated on Ultrogel and the elution was monitored by immunoassay of IgA, IgM and IgG, as well as by the two inhibition tests. The inhibiting factors were distributed over several peaks which only partially coincided with the elution profiles of IgG and IgM.", "contents": "Detection of circulating antigen-antibody complexes by their inhibitory effect on the agglutination of IgG-coated particles by rheumatioid factor of Clq. The agglutination of Ig-coated particles by human RF or Clq can be inhibited by Ig aggregates or AgAb complexes. The effect of Ig class was studied by means of agarose-linked human monoclonal Igs. RF was inhibited by all subclasses of IgG and IgA but not by IgM, whereas Clq reacted with IgM, IgG3 and IgG1. Heat-aggregated IgG3 was fractionated by gel-filtration on Ultrogel. Inhibition was restricted to certain fractions of aggregates, viz (IgG3) approximately 7 and (IgG3) approximately 21 for RF, and (IgG3) approximately 10, (IgG3) approximately 14 and (IgG3) approximately 27 for Clq. In a precipitin curve experiment, it was found that RF was inhibited by soluble complexes over an extended range of AgAb ratios, the inactivation of Clq being limited to complexes with 2-5 times antigen excess. Inhibiting factors were found in patients with various diseases and, at low titres, in 22% of healthy people. In 27% of patients' sera, the inhibitors were demonstrable by Clq only after removal of endogenous RF by adsorption on insolubilized IgG. In several patients endogenous agglutinating activity and direct inhibitory activity tended to alternate during the course of the disease. Sera from various patients were also filtrated on Ultrogel and the elution was monitored by immunoassay of IgA, IgM and IgG, as well as by the two inhibition tests. The inhibiting factors were distributed over several peaks which only partially coincided with the elution profiles of IgG and IgM.", "PMID": 954232}
{"id": "pubmed23n0032_1002", "title": "Immunoglobulins and transient paraproteins in sera of patients with the Wiskott-Aldrich syndrome: a follow-up study.", "content": "Immunoglobulin levels of individual classes and IgG subclasses and the occurrence of homogeneous immunoglobulins--paraproteins--were studied longitudinally in the sera of three patients with the Wiskott-Aldrich syndrome; Common findings in all three patients were great variations in the immunoglobulin levels, restricted heterogeneity of the immunoglobulins, the frequent appearance of transient homogeneous immunoglobulins and the presence of serum antibodies against bovine milk proteins. A partial and selective deficiency involving mainly the T immune system is postulated as an explanation for these findings.", "contents": "Immunoglobulins and transient paraproteins in sera of patients with the Wiskott-Aldrich syndrome: a follow-up study. Immunoglobulin levels of individual classes and IgG subclasses and the occurrence of homogeneous immunoglobulins--paraproteins--were studied longitudinally in the sera of three patients with the Wiskott-Aldrich syndrome; Common findings in all three patients were great variations in the immunoglobulin levels, restricted heterogeneity of the immunoglobulins, the frequent appearance of transient homogeneous immunoglobulins and the presence of serum antibodies against bovine milk proteins. A partial and selective deficiency involving mainly the T immune system is postulated as an explanation for these findings.", "PMID": 954233}
{"id": "pubmed23n0032_1003", "title": "Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes.", "content": "Following incubation with polyclonal activators such as PHA or concanavalin A, human peripheral blood lymphocytes show directional migration towards chemotactic factors such as endotoxin-activated plasma, casein or denatured proteins. This migration is inhibited by treatment of the cells with phospholipase C and sphingomyelinase C, but little affected by proteases or glycosidases. In addition, these lymphocytes migrate towards PHA and other lymphocyte activators when these reagents are used as chemoattractants at concentrations well below their mitogenic doses. They also migrate towards staphylococcal protein A. Migration towards PHA and protein A is reduced by pretreating the lymphocytes with proteases but not with phospholipase C. These results suggest two independent membrane interactions which initiate lymphocyte chemotaxis, one acting directly via the lipid bilayer, the other involving the binding of ligands to membrane proteins.", "contents": "Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes. Following incubation with polyclonal activators such as PHA or concanavalin A, human peripheral blood lymphocytes show directional migration towards chemotactic factors such as endotoxin-activated plasma, casein or denatured proteins. This migration is inhibited by treatment of the cells with phospholipase C and sphingomyelinase C, but little affected by proteases or glycosidases. In addition, these lymphocytes migrate towards PHA and other lymphocyte activators when these reagents are used as chemoattractants at concentrations well below their mitogenic doses. They also migrate towards staphylococcal protein A. Migration towards PHA and protein A is reduced by pretreating the lymphocytes with proteases but not with phospholipase C. These results suggest two independent membrane interactions which initiate lymphocyte chemotaxis, one acting directly via the lipid bilayer, the other involving the binding of ligands to membrane proteins.", "PMID": 954234}
{"id": "pubmed23n0032_1004", "title": "Molecular size of IgG in patients with focal glomerulosclerosis.", "content": "Nineteen patients with focal glomerulosclerosis and with anomalously high clearance of IgG into the urine have been investigated by G.200 column chromatography. The findings are compared with normal sera, with 14 patients with minimal change disease and with 12 patients with proliferative glomerulonephritis. No significant reduction in the molecular size of IgG was found as compared with normal sera and the other diseases in 18 of the patients. In patients with minimal change disease and focal glomerulosclerosis an increased proportion of the IgG eluted in the void volume", "contents": "Molecular size of IgG in patients with focal glomerulosclerosis. Nineteen patients with focal glomerulosclerosis and with anomalously high clearance of IgG into the urine have been investigated by G.200 column chromatography. The findings are compared with normal sera, with 14 patients with minimal change disease and with 12 patients with proliferative glomerulonephritis. No significant reduction in the molecular size of IgG was found as compared with normal sera and the other diseases in 18 of the patients. In patients with minimal change disease and focal glomerulosclerosis an increased proportion of the IgG eluted in the void volume", "PMID": 954235}
{"id": "pubmed23n0032_1005", "title": "Uremic pericarditis: a prospective echocardiographic and clinical study.", "content": "During a 14 month period 59 male patients with chronic renal failure who were candidates for chronic hemodialysis (HD) were evaluated clinically and echocardiographically for pericarditis. All were evaluated prospectively prior to or at the initiation of HD. Definite pericarditis was present in 8, all of whom were severely uremic and required initiation of HD on a semiemergent basis rather than electively (i.e., preselected level of renal function). In 6 of these 8, pericardial effusion responded to dialysis alone, one required pericardiectomy because of hypotension complicating dialysis, and one expired during a right atriogram. Patients dialyzed on an elective basis were all free of pericarditis at the initiation of HD. Pericarditis arising some months after the initiation of HD was a less frequent problem. It is concluded that (a) the incidence of pericarditis in the uremic state is decreased by early initiation of HD before advanced uremic symptoms have developed; (b) pericarditis present at the initiation of HD usually but not always is resolved with the initiation of HD; (c) echocardiography is an important clinical and epidemiological toole to investigate pericarditis in uremic patient populations.", "contents": "Uremic pericarditis: a prospective echocardiographic and clinical study. During a 14 month period 59 male patients with chronic renal failure who were candidates for chronic hemodialysis (HD) were evaluated clinically and echocardiographically for pericarditis. All were evaluated prospectively prior to or at the initiation of HD. Definite pericarditis was present in 8, all of whom were severely uremic and required initiation of HD on a semiemergent basis rather than electively (i.e., preselected level of renal function). In 6 of these 8, pericardial effusion responded to dialysis alone, one required pericardiectomy because of hypotension complicating dialysis, and one expired during a right atriogram. Patients dialyzed on an elective basis were all free of pericarditis at the initiation of HD. Pericarditis arising some months after the initiation of HD was a less frequent problem. It is concluded that (a) the incidence of pericarditis in the uremic state is decreased by early initiation of HD before advanced uremic symptoms have developed; (b) pericarditis present at the initiation of HD usually but not always is resolved with the initiation of HD; (c) echocardiography is an important clinical and epidemiological toole to investigate pericarditis in uremic patient populations.", "PMID": 954236}
{"id": "pubmed23n0032_1006", "title": "Unusual aspects of the dialysis disequilibrium syndrome.", "content": "In five patients with chronic renal failure, rapid correction by dialysis of hypertension and/or high blood urea levels provoked acute neurological disorders, followed by slowly reversible neuropsychiatric disturbances. Focal EEG alterations were noted in three patients with normal carotid angiograms. Our cases differed from those usually described as suffering from the dialysis disequilibrium syndrome because of their duration, the severity of mental disturbances, and the asymmetrical pattern of EEG abnormalities. We propose that the symptoms observed could be due to cerebral ischemia. This possibility emphasizes the importance of limiting the duration and efficiency of the first dialyses in patients with severe hypertension and high nitrogen retention, especially if high performance dialyzers are used.", "contents": "Unusual aspects of the dialysis disequilibrium syndrome. In five patients with chronic renal failure, rapid correction by dialysis of hypertension and/or high blood urea levels provoked acute neurological disorders, followed by slowly reversible neuropsychiatric disturbances. Focal EEG alterations were noted in three patients with normal carotid angiograms. Our cases differed from those usually described as suffering from the dialysis disequilibrium syndrome because of their duration, the severity of mental disturbances, and the asymmetrical pattern of EEG abnormalities. We propose that the symptoms observed could be due to cerebral ischemia. This possibility emphasizes the importance of limiting the duration and efficiency of the first dialyses in patients with severe hypertension and high nitrogen retention, especially if high performance dialyzers are used.", "PMID": 954237}
{"id": "pubmed23n0032_1007", "title": "Sexual behaviour of hemodialyzed patients.", "content": "18 married men on home dialysis and 10 of their wives were interviewed (structured interview) to evaluate sexual adjustment. The frequency of intercourse prior to the onset of their disease was reported to be 3.4/week by the patients and 2.4/week by their wives. During maintenance hemodialysis, frequency was reported to be 1.7/week by the patients and 1.0/week by their wives. If libido prior to the disease was rated 100%, the patients reported their libido at the time of the interview as 66+/-34%; only 5 rated their libido as less than 50% of their libido prior to the onset of their disease. After the start of dialysis, considerable time was required before a stable state of sexual activity was reached (13.9+/-7.4 months), presumably reflecting the time required for adaptation to the procedure, loss of anxiety and restoration of physical well-being. Except in older patients, there was good agreement between the feeling of physical fitness as reported by the patients and sexual performance as reported by both the patient and his spouse.", "contents": "Sexual behaviour of hemodialyzed patients. 18 married men on home dialysis and 10 of their wives were interviewed (structured interview) to evaluate sexual adjustment. The frequency of intercourse prior to the onset of their disease was reported to be 3.4/week by the patients and 2.4/week by their wives. During maintenance hemodialysis, frequency was reported to be 1.7/week by the patients and 1.0/week by their wives. If libido prior to the disease was rated 100%, the patients reported their libido at the time of the interview as 66+/-34%; only 5 rated their libido as less than 50% of their libido prior to the onset of their disease. After the start of dialysis, considerable time was required before a stable state of sexual activity was reached (13.9+/-7.4 months), presumably reflecting the time required for adaptation to the procedure, loss of anxiety and restoration of physical well-being. Except in older patients, there was good agreement between the feeling of physical fitness as reported by the patients and sexual performance as reported by both the patient and his spouse.", "PMID": 954238}
{"id": "pubmed23n0032_1008", "title": "Hemosiderosis in a patient on regular hemodialysis: treatment by desferrioxamine.", "content": "Hemosiderosis following regular administration of parenteral iron was observed in a patient receiving maintenance hemodialysis. Infusions of desferrioxamine in doses of 2,3 and 4 g each resulted in the removal of approximately 45 mg of iron during dialysis. Desferrioxamine 2 g was infused thrice weekly during dialysis for twelve months. Body iron stores, as judged by liver iron and serum ferritin concentrations, fell by about half. This agrees well with the result calculated from the amount of iron administered and the amount removed during dialysis.", "contents": "Hemosiderosis in a patient on regular hemodialysis: treatment by desferrioxamine. Hemosiderosis following regular administration of parenteral iron was observed in a patient receiving maintenance hemodialysis. Infusions of desferrioxamine in doses of 2,3 and 4 g each resulted in the removal of approximately 45 mg of iron during dialysis. Desferrioxamine 2 g was infused thrice weekly during dialysis for twelve months. Body iron stores, as judged by liver iron and serum ferritin concentrations, fell by about half. This agrees well with the result calculated from the amount of iron administered and the amount removed during dialysis.", "PMID": 954239}
{"id": "pubmed23n0032_1009", "title": "Scanning electron microscopy of the urinary tract.", "content": "Scanning electron microscopy of the urinary tract. A scanning electron microscopic investigation of the luminal surface of the entire urinary tract of the Wistar rat was performed. This report defines criteria for identifying different segments of normal urinary tract on the basis of scanning electron microscopic observations of the epithelial surface. The study was undertaken to provide a reference for future studies of changes in urinary tract morphology resulting from injury or disease.", "contents": "Scanning electron microscopy of the urinary tract. Scanning electron microscopy of the urinary tract. A scanning electron microscopic investigation of the luminal surface of the entire urinary tract of the Wistar rat was performed. This report defines criteria for identifying different segments of normal urinary tract on the basis of scanning electron microscopic observations of the epithelial surface. The study was undertaken to provide a reference for future studies of changes in urinary tract morphology resulting from injury or disease.", "PMID": 954240}
{"id": "pubmed23n0032_1010", "title": "Platelet function in patients on long term peritoneal dialysis.", "content": "Platelet function studies were performed on 18 patients with end stage chronic renal failure receiving treatment by long-term peritoneal dialysis. The results of these studies were compared with previously reported data on healthy volunteers, non-dialyzed patients with chronic renal failure and patients treated by hemodialysis. Platelet adhesiveness was impaired and, unlike the other patient groups, there was no relationship between adhesiveness and serum creatinine. Platelet aggregation to both ADP and adrenaline chloride was normal, whereas marked impairment was seen in non-dialyzed patients with severe chronic renal failure and patients receiving hemodialysis. The results suggest that different uremic retention products affect these different phases in the platelet adhesion-aggregation reaction and that the peritoneal membrane is more permeable to that dialyzable factor which inhibits aggregation than conventional hemodialysis membranes. The studies are of interest both in consideration of adequacy of dialysis and in studies into the nature of the platelet defect in uremia.", "contents": "Platelet function in patients on long term peritoneal dialysis. Platelet function studies were performed on 18 patients with end stage chronic renal failure receiving treatment by long-term peritoneal dialysis. The results of these studies were compared with previously reported data on healthy volunteers, non-dialyzed patients with chronic renal failure and patients treated by hemodialysis. Platelet adhesiveness was impaired and, unlike the other patient groups, there was no relationship between adhesiveness and serum creatinine. Platelet aggregation to both ADP and adrenaline chloride was normal, whereas marked impairment was seen in non-dialyzed patients with severe chronic renal failure and patients receiving hemodialysis. The results suggest that different uremic retention products affect these different phases in the platelet adhesion-aggregation reaction and that the peritoneal membrane is more permeable to that dialyzable factor which inhibits aggregation than conventional hemodialysis membranes. The studies are of interest both in consideration of adequacy of dialysis and in studies into the nature of the platelet defect in uremia.", "PMID": 954241}
{"id": "pubmed23n0032_1011", "title": "Renal tubular defects in recurring bilateral nephrolithiasis.", "content": "A metabolic study was performed in 28 patients with bilateral recurrent calcium-containing renal staghorn calculi and chronic pyelonephritis (\"obstructive nephropathy\"). Fourteen had normal GFR and 14 mild renal insufficiency. Ten normal subjects were used as controls. Under basal conditions, polyuria and negative sodium balance were commonly observed in patients with obstructive nephropathy and normal renal function. After an acute acid load (NH4Cl) an acidifying defect, i.e. high values for urine pH and reduced excretion of titratable acid and ammonium, was observed in 64% of patients with normal GFR and in 71.4% of those with renal insufficiency. During intravenous infusion with neutral sodium phosphate, the urine pH changed little but the rate of excretion of titratable acid increased in direct proportion to that of urinary phosphate in both groups of patients. These results, associated with the finding of normal blood pH in almost all patients, lead to the conclusion that an incomplete Type 1 or \"distal\" renal tubular acidosis is a frequent complication of obstructive nephropathy secondary to bilateral nephrolithiasis. The anatomical abnormalities of renal tubules and collecting ducts and the superimposed interstitial nephritis might be the pathogenetic factors responsible for the acidifying defect and for the impairment in sodium and water conservation.", "contents": "Renal tubular defects in recurring bilateral nephrolithiasis. A metabolic study was performed in 28 patients with bilateral recurrent calcium-containing renal staghorn calculi and chronic pyelonephritis (\"obstructive nephropathy\"). Fourteen had normal GFR and 14 mild renal insufficiency. Ten normal subjects were used as controls. Under basal conditions, polyuria and negative sodium balance were commonly observed in patients with obstructive nephropathy and normal renal function. After an acute acid load (NH4Cl) an acidifying defect, i.e. high values for urine pH and reduced excretion of titratable acid and ammonium, was observed in 64% of patients with normal GFR and in 71.4% of those with renal insufficiency. During intravenous infusion with neutral sodium phosphate, the urine pH changed little but the rate of excretion of titratable acid increased in direct proportion to that of urinary phosphate in both groups of patients. These results, associated with the finding of normal blood pH in almost all patients, lead to the conclusion that an incomplete Type 1 or \"distal\" renal tubular acidosis is a frequent complication of obstructive nephropathy secondary to bilateral nephrolithiasis. The anatomical abnormalities of renal tubules and collecting ducts and the superimposed interstitial nephritis might be the pathogenetic factors responsible for the acidifying defect and for the impairment in sodium and water conservation.", "PMID": 954242}
{"id": "pubmed23n0032_1012", "title": "Free phenols in chronic renal failure.", "content": "Serum total free phenols are elevated in chronic renal failure, acute renal failure and hepatic coma. Being partly protein-bound, phenols behave during dialysis in a similar manner to considerably larger molecules which are not protein-bound. In view of their potential toxicity they should be considered as an alternative to 'middle molecules'. Patients on regular hemodialysis have retention of phenols if their post-dialysis serum creatinine is above 6-7 mg/dl. Patients on short time dialysis have high pre-dialysis neutral phenol levels. Such levels are sufficiently high to suggest a role in the genesis of neurological symptoms, anemia and bone disease. Certainly pre-dialysis free phenols reflect adequacy of dialysis.", "contents": "Free phenols in chronic renal failure. Serum total free phenols are elevated in chronic renal failure, acute renal failure and hepatic coma. Being partly protein-bound, phenols behave during dialysis in a similar manner to considerably larger molecules which are not protein-bound. In view of their potential toxicity they should be considered as an alternative to 'middle molecules'. Patients on regular hemodialysis have retention of phenols if their post-dialysis serum creatinine is above 6-7 mg/dl. Patients on short time dialysis have high pre-dialysis neutral phenol levels. Such levels are sufficiently high to suggest a role in the genesis of neurological symptoms, anemia and bone disease. Certainly pre-dialysis free phenols reflect adequacy of dialysis.", "PMID": 954243}
{"id": "pubmed23n0032_1013", "title": "Isoniazid pharmacokinetics in patients in chronic renal failure.", "content": "INH pharmcokinetics was studied in 2 groups of patients in chronic renal failure. In the group on conservative treatment, INH half life was prolonged, but was not related to the degree on renal impairement. In the hemodialysis group 73% of the INH given was removed by dialysis within 5 hours. If INH is to be used in the presence of renal failure, individual half life estimations should be performed in order to determine dosage frequency. In patients on hemodialysis, therapy should be given post dialysis. Alternatively, drugs not dependent on renal failure, e.g. rifampicin, may be preferable.", "contents": "Isoniazid pharmacokinetics in patients in chronic renal failure. INH pharmcokinetics was studied in 2 groups of patients in chronic renal failure. In the group on conservative treatment, INH half life was prolonged, but was not related to the degree on renal impairement. In the hemodialysis group 73% of the INH given was removed by dialysis within 5 hours. If INH is to be used in the presence of renal failure, individual half life estimations should be performed in order to determine dosage frequency. In patients on hemodialysis, therapy should be given post dialysis. Alternatively, drugs not dependent on renal failure, e.g. rifampicin, may be preferable.", "PMID": 954244}
{"id": "pubmed23n0032_1014", "title": "Pleural dialysis in acute renal failure.", "content": "An 80 day old infant developed acute renal failure postoperatively after nephrectomy for Wilm's tumor. Pleural dialysis was utilized to control electrolyte imbalance of acute renal failure. Function of pleura as a dialyzing membrane was evaluated for the clearance of urea and creatinine.", "contents": "Pleural dialysis in acute renal failure. An 80 day old infant developed acute renal failure postoperatively after nephrectomy for Wilm's tumor. Pleural dialysis was utilized to control electrolyte imbalance of acute renal failure. Function of pleura as a dialyzing membrane was evaluated for the clearance of urea and creatinine.", "PMID": 954245}
{"id": "pubmed23n0032_1015", "title": "Life-threatening infections in pregnancy.", "content": "Infection remains a major cause of maternal morbidity and mortality. Those entities that are most likely to threaten the life of the pregnant woman have been reviewed. Although intrapartum and postpartum pelvic infections consitute the majority such serious infections in pregnancy, attention has also been called to several less frequent but potentially lethal nonpelvic infections.", "contents": "Life-threatening infections in pregnancy. Infection remains a major cause of maternal morbidity and mortality. Those entities that are most likely to threaten the life of the pregnant woman have been reviewed. Although intrapartum and postpartum pelvic infections consitute the majority such serious infections in pregnancy, attention has also been called to several less frequent but potentially lethal nonpelvic infections.", "PMID": 954250}
{"id": "pubmed23n0032_1016", "title": "Difficulties in delivery, including shoulder dystocia and malpresentations of the fetus.", "content": "Shoulder dystocia is a true obsteric emergency. It may be suspected in large multiparous women, women who have previously delivered large babies, prolonged pregnancy, women who make poor progress in labor, and when the fetus seems very large to palpation. Women with abnormal pelves should be suspect. More liberal use of cesarean section in these situations will reduce the incidence of shoulder dystocia. It is important to have a carefully considered plan of action if shoulder dystocia does occur. The most helpful and definitive maneuver is extration of the posterior arm. Malpresentations cause acute emergencies mainly because of the associated increased incidence of prolapse of the cord. This possibility should be evaluated immediately when rupture of the membranes occurs. Prompt delivery by cesarean section is the treatment of choice with this complications.", "contents": "Difficulties in delivery, including shoulder dystocia and malpresentations of the fetus. Shoulder dystocia is a true obsteric emergency. It may be suspected in large multiparous women, women who have previously delivered large babies, prolonged pregnancy, women who make poor progress in labor, and when the fetus seems very large to palpation. Women with abnormal pelves should be suspect. More liberal use of cesarean section in these situations will reduce the incidence of shoulder dystocia. It is important to have a carefully considered plan of action if shoulder dystocia does occur. The most helpful and definitive maneuver is extration of the posterior arm. Malpresentations cause acute emergencies mainly because of the associated increased incidence of prolapse of the cord. This possibility should be evaluated immediately when rupture of the membranes occurs. Prompt delivery by cesarean section is the treatment of choice with this complications.", "PMID": 954251}
{"id": "pubmed23n0032_1017", "title": "Ureteral injuries associated with gynecologic surgery: prevention and management.", "content": "Gynecologic surgery is responsible for most of the ureteral injuries that occur. The \"easy\" operation--the \"simple\" abdominal hysterectomy--and not the technically difficult pelvic one, is responsible for most ureteral injuries. Total abdominal hysterectomy accounts for almost 50% of the genitourinary fistulas and perhaps 80-99% of all surgical ureteral injuries. This problem will persist until a most important surgical axiom is applied routinely during the accomplishment of all pelvic operations: With all dissections, the contiguous structures subject to injury must be exposed. This step not only will avoid injuries to the ureter but also will facilitate an equally important aspect, that is, urinary tract injuries must be recognized at the time of operation. With recognition and adequate repair, problems such as fistula formation and serious morbidity (and litigation) can be avoided almost entirely. Because the gnecologic surgeon frequently will find that urologic consultation is not available at the time of urinary tract injury, he or she must be aware of and familiar with the various ureteral reconstructive procedures that may be required. The gynecologic surgeon must devote time and study to the management of urinary tract injuries before their occurrence. All pelvic surgeons eventually will encounter ureteral problems. The methods of bladder mobilization and ureteroneocystostomy should be within the ability of all who operate within the pelvis. When extensive damage has occurred and a urologist is not available, the gynecologist who is unfamiliar with the more demanding techniques (that is, ureteroureterostomy, bladder flaps, ileal conduits) should avoid additonal damage to the urinary tract and accomplish a simple catheter ureterostomy, deffering the definitive repair for a urologist.", "contents": "Ureteral injuries associated with gynecologic surgery: prevention and management. Gynecologic surgery is responsible for most of the ureteral injuries that occur. The \"easy\" operation--the \"simple\" abdominal hysterectomy--and not the technically difficult pelvic one, is responsible for most ureteral injuries. Total abdominal hysterectomy accounts for almost 50% of the genitourinary fistulas and perhaps 80-99% of all surgical ureteral injuries. This problem will persist until a most important surgical axiom is applied routinely during the accomplishment of all pelvic operations: With all dissections, the contiguous structures subject to injury must be exposed. This step not only will avoid injuries to the ureter but also will facilitate an equally important aspect, that is, urinary tract injuries must be recognized at the time of operation. With recognition and adequate repair, problems such as fistula formation and serious morbidity (and litigation) can be avoided almost entirely. Because the gnecologic surgeon frequently will find that urologic consultation is not available at the time of urinary tract injury, he or she must be aware of and familiar with the various ureteral reconstructive procedures that may be required. The gynecologic surgeon must devote time and study to the management of urinary tract injuries before their occurrence. All pelvic surgeons eventually will encounter ureteral problems. The methods of bladder mobilization and ureteroneocystostomy should be within the ability of all who operate within the pelvis. When extensive damage has occurred and a urologist is not available, the gynecologist who is unfamiliar with the more demanding techniques (that is, ureteroureterostomy, bladder flaps, ileal conduits) should avoid additonal damage to the urinary tract and accomplish a simple catheter ureterostomy, deffering the definitive repair for a urologist.", "PMID": 954253}
{"id": "pubmed23n0032_1018", "title": "Management of ovarian carcinoma in young women.", "content": "On the basis of available information, the following criteria regarding ovarian carcinoma may be considered for the young patient who has not completed her family and is desirous of further childbearing. Any ovarian enlargements must be promptly and adequately evaluated. A lesion of high grade or high stage is not amenable to conservative surgery, and definitive total abdominal hysterectomy, bilateral salpinog-oophorectomy with or without partial omentectomy, and subsequent chemotherapy or radiotherapy are indicated. In the individual who has a low-grade and intracystic or encapsulated ovarian neoplasm, conservative surgery may be considered, provided there is no evidence otherwise of any periotneal or other spread and particularly if the opposite ovary is histologically evaluated, preferably by wedge resection. Data have indicated that this is a reasonable process to consider, and if followed carefully this should allow these individuals to accomplish their childbearing function with minimal risk. In general, because of the bilaterality of ovarian tumors, such conservative surgery probably is indefensible unless these riged criteria are met. It would also seem preferable to complete the surgical procedure with hysterectomy and salpingo-oophorectomy once the patient's family is complete, thus obviating any possible appearance of a malignant tumor in the remaining ovary. The use of exogenous estrogesn would also seem far preferable to the risk of an insidious low-grade tumor that could not be adequately followed.", "contents": "Management of ovarian carcinoma in young women. On the basis of available information, the following criteria regarding ovarian carcinoma may be considered for the young patient who has not completed her family and is desirous of further childbearing. Any ovarian enlargements must be promptly and adequately evaluated. A lesion of high grade or high stage is not amenable to conservative surgery, and definitive total abdominal hysterectomy, bilateral salpinog-oophorectomy with or without partial omentectomy, and subsequent chemotherapy or radiotherapy are indicated. In the individual who has a low-grade and intracystic or encapsulated ovarian neoplasm, conservative surgery may be considered, provided there is no evidence otherwise of any periotneal or other spread and particularly if the opposite ovary is histologically evaluated, preferably by wedge resection. Data have indicated that this is a reasonable process to consider, and if followed carefully this should allow these individuals to accomplish their childbearing function with minimal risk. In general, because of the bilaterality of ovarian tumors, such conservative surgery probably is indefensible unless these riged criteria are met. It would also seem preferable to complete the surgical procedure with hysterectomy and salpingo-oophorectomy once the patient's family is complete, thus obviating any possible appearance of a malignant tumor in the remaining ovary. The use of exogenous estrogesn would also seem far preferable to the risk of an insidious low-grade tumor that could not be adequately followed.", "PMID": 954255}
{"id": "pubmed23n0032_1019", "title": "Scheuermann's kyphosis: a form of osteoporosis?", "content": "A high frequency of abnormalities were noted in radiographic measurements of bone density, microradiography of the iliac crest, serum alkaline phosphatase, urinary hydroxyproline and dietary calcium intake in 12 patients with Scheuermann's juvenile kyphosis. Scheuermann's kyphosis may be a manifestation of generalized skeletal disease which becomes symptomatic during the growth spurt of puberty.", "contents": "Scheuermann's kyphosis: a form of osteoporosis? A high frequency of abnormalities were noted in radiographic measurements of bone density, microradiography of the iliac crest, serum alkaline phosphatase, urinary hydroxyproline and dietary calcium intake in 12 patients with Scheuermann's juvenile kyphosis. Scheuermann's kyphosis may be a manifestation of generalized skeletal disease which becomes symptomatic during the growth spurt of puberty.", "PMID": 954261}
{"id": "pubmed23n0032_1020", "title": "The clinical biomechanics of scoliosis.", "content": "Removal of the posterior elements will allow increased correction of axial deformity, in scoliosis. The clinician may take advantage of the creep and relaxation characteristic of the tissues to improve efficiency of correction. Axial loading has been shown theoretically to be more efficient for the more severe curves, (greater than 53 degrees) and transverse loading more efficient for the less severe curves (less than 53 degrees). Combined loading is always more efficient than either type alone. The Milwaukee brace can be just as effective as a cast in resisting deforming forces in scoliosis. Removal of axillary supports or thoracic pads or not wearing the brace when recumbent reduces the effectiveness of the Milwaukee brace. The strength of the thoracid lamina is a limiting factor in the amount of forces that may be applied to correct the deformity; 30 kilopond (65.8 lb) is the upper limit of this force. Coughing or buckling can apply dangerously high forces with the Harrington rod. Greater surgace contact of the hook to the lamina and small increments between notches on the rod may increase the tolerance limits of the system. Compression rods on the convex side probably add little or no correctional value. The Dwyer technique is biomechanically sound and effective and has the additional advantage of applying asymmetrical loads to the epiphyseal plates.", "contents": "The clinical biomechanics of scoliosis. Removal of the posterior elements will allow increased correction of axial deformity, in scoliosis. The clinician may take advantage of the creep and relaxation characteristic of the tissues to improve efficiency of correction. Axial loading has been shown theoretically to be more efficient for the more severe curves, (greater than 53 degrees) and transverse loading more efficient for the less severe curves (less than 53 degrees). Combined loading is always more efficient than either type alone. The Milwaukee brace can be just as effective as a cast in resisting deforming forces in scoliosis. Removal of axillary supports or thoracic pads or not wearing the brace when recumbent reduces the effectiveness of the Milwaukee brace. The strength of the thoracid lamina is a limiting factor in the amount of forces that may be applied to correct the deformity; 30 kilopond (65.8 lb) is the upper limit of this force. Coughing or buckling can apply dangerously high forces with the Harrington rod. Greater surgace contact of the hook to the lamina and small increments between notches on the rod may increase the tolerance limits of the system. Compression rods on the convex side probably add little or no correctional value. The Dwyer technique is biomechanically sound and effective and has the additional advantage of applying asymmetrical loads to the epiphyseal plates.", "PMID": 954262}
{"id": "pubmed23n0032_1021", "title": "Pyogenic infections of the sacro-iliac joint.", "content": "In 3 cases of pyogenic infection of the sacro-iliac joint, the diagnosis was difficult. Limp, buttock pain and upper sciatica were the most common presenting symptoms. Differential limitation of hip motion, positive straight leg raising, a positive Gaenslen test and pain on pelvic compression were frequent fingings. Increased E.S.R., W.B.C. count and fever were seen in 2/3 cases. Plain and tomographic X-rays are often helpful but bone scan has been helpful in localizing early lesions. Treatment after early diagnosis may consist of antibiotics and immobilization alone while in the more chronic cases surgical drainage and debridement may be necessary.", "contents": "Pyogenic infections of the sacro-iliac joint. In 3 cases of pyogenic infection of the sacro-iliac joint, the diagnosis was difficult. Limp, buttock pain and upper sciatica were the most common presenting symptoms. Differential limitation of hip motion, positive straight leg raising, a positive Gaenslen test and pain on pelvic compression were frequent fingings. Increased E.S.R., W.B.C. count and fever were seen in 2/3 cases. Plain and tomographic X-rays are often helpful but bone scan has been helpful in localizing early lesions. Treatment after early diagnosis may consist of antibiotics and immobilization alone while in the more chronic cases surgical drainage and debridement may be necessary.", "PMID": 954263}
{"id": "pubmed23n0032_1022", "title": "Bone injuries due to tetany or convulsions during hemodialysis.", "content": "Two patients with long standing uremia sustained bone injuries due to tetany in one, and convulsions secondary to hemodialysis-induced alkalosis and hypomagnesemia in the other. In the former, there were multiple fractures involving the scapula, clavicles and femoral necks. In the latter, there was a slipped capital femoral epiphysis. Caution should be exercised against tetany or convulsive episodes which may cause bone injuries during hemodiaylsis in severely osteoporotic patients. In patients with progressive renal osteodystrophy, prevention of severe osteopenia by timely kidney transplant or subtotal parathyroidectomy is an important preventative measure.", "contents": "Bone injuries due to tetany or convulsions during hemodialysis. Two patients with long standing uremia sustained bone injuries due to tetany in one, and convulsions secondary to hemodialysis-induced alkalosis and hypomagnesemia in the other. In the former, there were multiple fractures involving the scapula, clavicles and femoral necks. In the latter, there was a slipped capital femoral epiphysis. Caution should be exercised against tetany or convulsive episodes which may cause bone injuries during hemodiaylsis in severely osteoporotic patients. In patients with progressive renal osteodystrophy, prevention of severe osteopenia by timely kidney transplant or subtotal parathyroidectomy is an important preventative measure.", "PMID": 954264}
{"id": "pubmed23n0032_1023", "title": "Candida arthritis of the knee joint.", "content": "Candida albicans arthritis is unusual but does occur perhaps more often than recognized. The most common site of infection is the knee joint in the adult and can be related to contamination of the joint at the time of intraarticular injection of corticosteroids.", "contents": "Candida arthritis of the knee joint. Candida albicans arthritis is unusual but does occur perhaps more often than recognized. The most common site of infection is the knee joint in the adult and can be related to contamination of the joint at the time of intraarticular injection of corticosteroids.", "PMID": 954265}
{"id": "pubmed23n0032_1024", "title": "Aneurysmal bone cyst: evaluation of resection and of curettage in 20 cases.", "content": "The clinical, angiographic and histological aspects of 20 cases of aneurysmal bone cyst suggest that angiography is a valuable aid in the diagnosis of a cyst, differentiating it from other cystic bone lesions. Angiographic information also has a certain bearing on the origin of the lesion. It was demonstrated that other, benign, lesions may exist adjacent to the cyst or prior to it; in most cases, however, the cyst appears in a bone which is otherwise normal. The failure to differentiate between aneurysmal bone cyst and giant cell tumor in relation to sex, age, localizaiton, morphology, and clinical course (e.g. tendency of recurrence and malignant transformation) may be responsible for confusion in the literature on these lesions. Good results of treatment are achievable by resection en bloc and by resection, or curettage with bone grafting. Curettage alone is inadequate. Only two recurrences (10%) were recorded in the present series during the average follow-up period of 4.5 years.", "contents": "Aneurysmal bone cyst: evaluation of resection and of curettage in 20 cases. The clinical, angiographic and histological aspects of 20 cases of aneurysmal bone cyst suggest that angiography is a valuable aid in the diagnosis of a cyst, differentiating it from other cystic bone lesions. Angiographic information also has a certain bearing on the origin of the lesion. It was demonstrated that other, benign, lesions may exist adjacent to the cyst or prior to it; in most cases, however, the cyst appears in a bone which is otherwise normal. The failure to differentiate between aneurysmal bone cyst and giant cell tumor in relation to sex, age, localizaiton, morphology, and clinical course (e.g. tendency of recurrence and malignant transformation) may be responsible for confusion in the literature on these lesions. Good results of treatment are achievable by resection en bloc and by resection, or curettage with bone grafting. Curettage alone is inadequate. Only two recurrences (10%) were recorded in the present series during the average follow-up period of 4.5 years.", "PMID": 954266}
{"id": "pubmed23n0032_1025", "title": "Solitary bone cyst with epiphyseal involvement: a case report.", "content": "A large solitary bone cyst involving the epiphysis produced 5.0 cm shortening of the proximal humerus in a 15-year-old girl. Biopsy revealed the cyst was filled with thin yellow fluid under pressure and lined by a fibrous membrane. X-rays 9 months after biopsy demonstrated progressive cyst obliteration. A review of literature revealed no similar case. It is postulated that solitary cysts rarely may involve the physeal and epiphyseal structures and thereby retard longitudinal bone growth.", "contents": "Solitary bone cyst with epiphyseal involvement: a case report. A large solitary bone cyst involving the epiphysis produced 5.0 cm shortening of the proximal humerus in a 15-year-old girl. Biopsy revealed the cyst was filled with thin yellow fluid under pressure and lined by a fibrous membrane. X-rays 9 months after biopsy demonstrated progressive cyst obliteration. A review of literature revealed no similar case. It is postulated that solitary cysts rarely may involve the physeal and epiphyseal structures and thereby retard longitudinal bone growth.", "PMID": 954267}
{"id": "pubmed23n0032_1026", "title": "Myositis ossificans traumatica in a young child: a case report.", "content": "This is a case report of a 2-year, 11-month-old boy with traumatic myositis ossificans of the deltoid muscle, possibly the youngest child with this condition reported in the literature. The lesion, removed 7 months after injury because of pain, showed no evidence of spontaneous regression.", "contents": "Myositis ossificans traumatica in a young child: a case report. This is a case report of a 2-year, 11-month-old boy with traumatic myositis ossificans of the deltoid muscle, possibly the youngest child with this condition reported in the literature. The lesion, removed 7 months after injury because of pain, showed no evidence of spontaneous regression.", "PMID": 954268}
{"id": "pubmed23n0032_1027", "title": "Eosinophilic granuloma of the proximal tibial epiphysis.", "content": "This is a 3-year follow-up report of an eosinophilic granuloma of bone occurring in a growing epiphysis. The lesion occurred in a 6-year-old boy and is only the third such case reported in the literature. Eosinophilic granuloma should be considered in the differential diagnosis of lytic lesions of the epiphysis in childhood.", "contents": "Eosinophilic granuloma of the proximal tibial epiphysis. This is a 3-year follow-up report of an eosinophilic granuloma of bone occurring in a growing epiphysis. The lesion occurred in a 6-year-old boy and is only the third such case reported in the literature. Eosinophilic granuloma should be considered in the differential diagnosis of lytic lesions of the epiphysis in childhood.", "PMID": 954269}
{"id": "pubmed23n0032_1028", "title": "Osteoid osteoma of the ulna in a Negro child: a case report.", "content": "Osteoid osteoma seems to have been reported only twice before in a Negro child. The lesion is also unusual in the ulna. Treatment by en bloc resection bone graft of a large surgical defect was successful.", "contents": "Osteoid osteoma of the ulna in a Negro child: a case report. Osteoid osteoma seems to have been reported only twice before in a Negro child. The lesion is also unusual in the ulna. Treatment by en bloc resection bone graft of a large surgical defect was successful.", "PMID": 954270}
{"id": "pubmed23n0032_1029", "title": "Osteoid osteoma of a vertebral body: report of a case with extension across the intervertebral disk.", "content": "An 18-year-old woman was evaluated for aching mid thoracid back pain of 4 years' duration. At 13 degrees left thoracic scoliosis was noted in association with a sclerotic process involving the inferior portion of the vertebral body of T7 and the superior margin of T8 across a well maintained disk space. Open biopsy confirmed the impression of a nidus in the body of T7 which was histologically consistent with osteoid osteoma. The disk space was well maintained and the sclerotic changes in the superior margin of T8 appeared to represent reactive change. Postoperative relief of back pain was complete. The occurrence of reactive bone across an intact disk space in response to an osteoid osteoma nidus is unique to this case. Osteoid osteoma should be included in the differential diagnosis of chronic spine pain and reactive changes secondary to irritant property of the nidus may be widespread.", "contents": "Osteoid osteoma of a vertebral body: report of a case with extension across the intervertebral disk. An 18-year-old woman was evaluated for aching mid thoracid back pain of 4 years' duration. At 13 degrees left thoracic scoliosis was noted in association with a sclerotic process involving the inferior portion of the vertebral body of T7 and the superior margin of T8 across a well maintained disk space. Open biopsy confirmed the impression of a nidus in the body of T7 which was histologically consistent with osteoid osteoma. The disk space was well maintained and the sclerotic changes in the superior margin of T8 appeared to represent reactive change. Postoperative relief of back pain was complete. The occurrence of reactive bone across an intact disk space in response to an osteoid osteoma nidus is unique to this case. Osteoid osteoma should be included in the differential diagnosis of chronic spine pain and reactive changes secondary to irritant property of the nidus may be widespread.", "PMID": 954271}
{"id": "pubmed23n0032_1030", "title": "Neurotrophic arthropathy of the shoulder secondary to tuberculous arachnoiditis: a case report.", "content": "A rapidly progressive neurotrophic arthropathy of the shoulder was noted in a 44-year-old man with tuberculous adhesive arachnoiditis. Difficulty in making the diagnosis of adhesive arachnoiditis was encountered because of the variable and confusing neurologic manifestations until a cisternal myelogram was performed and this previously unreported relationship established.", "contents": "Neurotrophic arthropathy of the shoulder secondary to tuberculous arachnoiditis: a case report. A rapidly progressive neurotrophic arthropathy of the shoulder was noted in a 44-year-old man with tuberculous adhesive arachnoiditis. Difficulty in making the diagnosis of adhesive arachnoiditis was encountered because of the variable and confusing neurologic manifestations until a cisternal myelogram was performed and this previously unreported relationship established.", "PMID": 954273}
{"id": "pubmed23n0032_1031", "title": "Calcifying tendinitis: a new concept of its pathogenesis.", "content": "To elucidate the pathogenesis of calcifying tendinitis, clinical and morphological investigations were done on 46 surgically treated cases. Contrary to the prevalent concept of degeneration preceding dystrophic calcification, we found no evidence for an active or a healed degenerative process. The affected tendon was transformed into fibrocartilage with a predilection for calcification. The formative phase of calcification was followed in course of time by a resorptive phase during which the deposits were surrounded by phagocytosing cells. There was a concomitant proliferation of vascular channels. We found a significant correlation between severe pain and histological signs of resorption. The pathogenetic mechanism of calcifying tendinitis should be reassessed as a unique disorder of the musculotendinous cuff.", "contents": "Calcifying tendinitis: a new concept of its pathogenesis. To elucidate the pathogenesis of calcifying tendinitis, clinical and morphological investigations were done on 46 surgically treated cases. Contrary to the prevalent concept of degeneration preceding dystrophic calcification, we found no evidence for an active or a healed degenerative process. The affected tendon was transformed into fibrocartilage with a predilection for calcification. The formative phase of calcification was followed in course of time by a resorptive phase during which the deposits were surrounded by phagocytosing cells. There was a concomitant proliferation of vascular channels. We found a significant correlation between severe pain and histological signs of resorption. The pathogenetic mechanism of calcifying tendinitis should be reassessed as a unique disorder of the musculotendinous cuff.", "PMID": 954272}
{"id": "pubmed23n0032_1032", "title": "Keratoacanthoma: an unusual hand tumor.", "content": "Keratoacanthoma is a common skin lesion of the head and neck, and is well described in the literature. It seldom presents to the Orthopedic surgeon as a hand tumor. The lesion does not require radical approach, but mimics squamous cell carcinoma which usually does. The clinical behavior and pathology, as well as the biological behavior of the tumor, are discussed. Fifteen cases treated by the authors are reported and their clinical and pathological course and treatment are discussed. Serious errors in treatment may arise from reliance on the histologic characteristics alone. Conservative treatment of keratoacanthoma should be avoided but radical excision of the part is unnecessary. Prompt and total excision is the treatment of choice, with closure either primarily or with split skin graft.", "contents": "Keratoacanthoma: an unusual hand tumor. Keratoacanthoma is a common skin lesion of the head and neck, and is well described in the literature. It seldom presents to the Orthopedic surgeon as a hand tumor. The lesion does not require radical approach, but mimics squamous cell carcinoma which usually does. The clinical behavior and pathology, as well as the biological behavior of the tumor, are discussed. Fifteen cases treated by the authors are reported and their clinical and pathological course and treatment are discussed. Serious errors in treatment may arise from reliance on the histologic characteristics alone. Conservative treatment of keratoacanthoma should be avoided but radical excision of the part is unnecessary. Prompt and total excision is the treatment of choice, with closure either primarily or with split skin graft.", "PMID": 954274}
{"id": "pubmed23n0032_1033", "title": "Bilateral carpal tunnel syndrome secondary to gouty tenosynovitis: a case report.", "content": "A 30-year-old patient developed bilateral carpal tunnel syndrome secondary to gouty tenosynovitis. Relief of symptoms followed removal of the tophaceous masses from the carpal tunnel. Surgical treatment is recommended whenever there is symptomatic median nerve compression.", "contents": "Bilateral carpal tunnel syndrome secondary to gouty tenosynovitis: a case report. A 30-year-old patient developed bilateral carpal tunnel syndrome secondary to gouty tenosynovitis. Relief of symptoms followed removal of the tophaceous masses from the carpal tunnel. Surgical treatment is recommended whenever there is symptomatic median nerve compression.", "PMID": 954275}
{"id": "pubmed23n0032_1034", "title": "The Milwaukee brace in idiopathic scoliosis: evaluation of 123 completed cases.", "content": "The Milwaukee Brace, as evaluated in 123 completed cases, is effective in controlling and correcting mild to moderate idiopathic scoliotic curves. The factors contributing to more correction include dorsal location, younger patients at onset of treatment, more flexible curves, and most important cooperative patients and parents. Less severe curves also achieved greater correction. Loss of correction after brace discontinuance is negligible, due to the fact that weaning and discontinuance were based on the stability of correction after 24 unbraced hours. Patients cooperative with the wearing and exercise program can expect approximately 25 per cent correction in dorsal curves and 20 per cent correction in lumbar curves. Cosmetic improvement can be expected in the majority of patients. Complications are negligible.", "contents": "The Milwaukee brace in idiopathic scoliosis: evaluation of 123 completed cases. The Milwaukee Brace, as evaluated in 123 completed cases, is effective in controlling and correcting mild to moderate idiopathic scoliotic curves. The factors contributing to more correction include dorsal location, younger patients at onset of treatment, more flexible curves, and most important cooperative patients and parents. Less severe curves also achieved greater correction. Loss of correction after brace discontinuance is negligible, due to the fact that weaning and discontinuance were based on the stability of correction after 24 unbraced hours. Patients cooperative with the wearing and exercise program can expect approximately 25 per cent correction in dorsal curves and 20 per cent correction in lumbar curves. Cosmetic improvement can be expected in the majority of patients. Complications are negligible.", "PMID": 954276}
{"id": "pubmed23n0032_1035", "title": "Vertebral body osteomyelitis: spectrum and natural history. A retrospective analysis of 37 cases.", "content": "There has been a definite change in the natural history of vertebral osteomyelitis. At the turn of the century the disease was most often seen in younger people, accompanied by a high incidence of abscess formation and associated with a high mortality rate. Today the disease is more commonly seen in the older age groups and is only occasionally characterized by abscess formation. In addition it tends to be of a lower grade inflammatory reaction and has a better prognosis. A urinary tract infection is a common pre-existing condition. Diagnosis may be difficult particularly before the onset of destructive changes radiologically at 8 to 12 weeks. Biopsy by either open or closed methods might be necessary to establishe the diagnosis and isolate the causative organism which is usually Staphylococcus aureus. Epidural infection is the most devastating complication, particularly if neglected. The duration of treatment is determined by following the clinical symptoms, sedimentation rate, temperature curve and interval radiological changes. The prognosis is good and most patients will recover within one year.", "contents": "Vertebral body osteomyelitis: spectrum and natural history. A retrospective analysis of 37 cases. There has been a definite change in the natural history of vertebral osteomyelitis. At the turn of the century the disease was most often seen in younger people, accompanied by a high incidence of abscess formation and associated with a high mortality rate. Today the disease is more commonly seen in the older age groups and is only occasionally characterized by abscess formation. In addition it tends to be of a lower grade inflammatory reaction and has a better prognosis. A urinary tract infection is a common pre-existing condition. Diagnosis may be difficult particularly before the onset of destructive changes radiologically at 8 to 12 weeks. Biopsy by either open or closed methods might be necessary to establishe the diagnosis and isolate the causative organism which is usually Staphylococcus aureus. Epidural infection is the most devastating complication, particularly if neglected. The duration of treatment is determined by following the clinical symptoms, sedimentation rate, temperature curve and interval radiological changes. The prognosis is good and most patients will recover within one year.", "PMID": 954277}
{"id": "pubmed23n0032_1036", "title": "Anatomical and functional considerations in total claviclectomy.", "content": "There should be no hesitation in performing a total claviclectomy. It is the treatment of choice in cases of ch. pyogenic osteomyelitis of the clavicle and other lesions which demand sacrificing the bone. There was no functional impairment of the shoulder joint or the girdle. There is usually no major postoperative complication and no residual disability. In children, regeneration of bone takes place from the periosteal tube.", "contents": "Anatomical and functional considerations in total claviclectomy. There should be no hesitation in performing a total claviclectomy. It is the treatment of choice in cases of ch. pyogenic osteomyelitis of the clavicle and other lesions which demand sacrificing the bone. There was no functional impairment of the shoulder joint or the girdle. There is usually no major postoperative complication and no residual disability. In children, regeneration of bone takes place from the periosteal tube.", "PMID": 954278}
{"id": "pubmed23n0032_1037", "title": "Posterior roentgenogram of the foot.", "content": "A present there is no reliable way to define the position of the heel in relationship to the tibia during weight bearing. Many surgical and nonsurgical procedures are done to correct a varus or valgus heel without a standard method to define the problem or measure the results. This is a report on a posterior roentgenographic technique which defines the position of the heel with respect to the talus, tibia, and forefoot. This technique allows for measurement of the position of the heel and can be performed in any X-ray facility.", "contents": "Posterior roentgenogram of the foot. A present there is no reliable way to define the position of the heel in relationship to the tibia during weight bearing. Many surgical and nonsurgical procedures are done to correct a varus or valgus heel without a standard method to define the problem or measure the results. This is a report on a posterior roentgenographic technique which defines the position of the heel with respect to the talus, tibia, and forefoot. This technique allows for measurement of the position of the heel and can be performed in any X-ray facility.", "PMID": 954279}
{"id": "pubmed23n0032_1038", "title": "Acidic lipids associated with the local mechanism of calcificaiton: a review.", "content": "Two current areas of research on hard tissues have focused upon acidic lipids associated with the local mechanism of calcification and the presence of matrix vesicles as the loci for initial mineralization. Data which show that acidic phospholipids are a component of the vesicle membrane provide a common denominator between these two areas. Furthermore, studies on vertebrate, microbial and synthetic lipoprotein calcification indicate that acidic phospholipids play a pivotal role in the local mechanism. Through a sequence of initial Ca2++ binding followed by desolvation and ion concentration, the acidic phospholipid rich membrane provides an environment in which calcification can be facilitated. A proposed mechanism includes in additon to Ca2++ binding, the formation of (CaHPO4)2 dimers and their condensation into a Ca9 (PO4)6 unit bound to the membrane. The bound unit functions as a nucleus for the formation of additonal units to form an amorphous calcium phosphate cluster. Conversion to crystalline apatite would require dehydration of the environment, either by mineral build up exceeding the hydrophobic domain or by breakdown of the membrane.", "contents": "Acidic lipids associated with the local mechanism of calcificaiton: a review. Two current areas of research on hard tissues have focused upon acidic lipids associated with the local mechanism of calcification and the presence of matrix vesicles as the loci for initial mineralization. Data which show that acidic phospholipids are a component of the vesicle membrane provide a common denominator between these two areas. Furthermore, studies on vertebrate, microbial and synthetic lipoprotein calcification indicate that acidic phospholipids play a pivotal role in the local mechanism. Through a sequence of initial Ca2++ binding followed by desolvation and ion concentration, the acidic phospholipid rich membrane provides an environment in which calcification can be facilitated. A proposed mechanism includes in additon to Ca2++ binding, the formation of (CaHPO4)2 dimers and their condensation into a Ca9 (PO4)6 unit bound to the membrane. The bound unit functions as a nucleus for the formation of additonal units to form an amorphous calcium phosphate cluster. Conversion to crystalline apatite would require dehydration of the environment, either by mineral build up exceeding the hydrophobic domain or by breakdown of the membrane.", "PMID": 954280}
{"id": "pubmed23n0032_1039", "title": "Intravenous regional anesthesia for closed treatment of fractures and dislocations of the upper extremities.", "content": "Intravenous regional anesthesia for the treatment of fractures and dislocations of the upper extremity is a very effective, consistent and safe form of analgesia which requires low doses of lidocaine and can be performed in an emergency room using a regular blood pressure cuff. Dosage should be related to body weight and the blood pressure cuff should be maintained at higher than systolic pressure for a minimum of 15 minutes after the lidocaine is injected. Release of the tourniquet should be staged as described. Ninety-one per cent of 77 patients had excellent analgesia following the IVRA. Eight per cent had fair results, but this was still adequate to perform the reduction with only minimal but definite discomfort to the patient. Only one patient failed to respond to the IVRA technique. Other advantages such as muscle relaxation during the anesthetic and rapid full return of sensation after cuff release, permit ease of reducion and early anticipation of cast discomfort or pressure pain from sharp edges of plaster. Unpleasant long term side effects of axillary block anesthesia, such as persistent paresthesia have not been seen.", "contents": "Intravenous regional anesthesia for closed treatment of fractures and dislocations of the upper extremities. Intravenous regional anesthesia for the treatment of fractures and dislocations of the upper extremity is a very effective, consistent and safe form of analgesia which requires low doses of lidocaine and can be performed in an emergency room using a regular blood pressure cuff. Dosage should be related to body weight and the blood pressure cuff should be maintained at higher than systolic pressure for a minimum of 15 minutes after the lidocaine is injected. Release of the tourniquet should be staged as described. Ninety-one per cent of 77 patients had excellent analgesia following the IVRA. Eight per cent had fair results, but this was still adequate to perform the reduction with only minimal but definite discomfort to the patient. Only one patient failed to respond to the IVRA technique. Other advantages such as muscle relaxation during the anesthetic and rapid full return of sensation after cuff release, permit ease of reducion and early anticipation of cast discomfort or pressure pain from sharp edges of plaster. Unpleasant long term side effects of axillary block anesthesia, such as persistent paresthesia have not been seen.", "PMID": 954282}
{"id": "pubmed23n0032_1040", "title": "The effect of thyroparathyroidectomy: development of disuse osteoporosis in adult rats.", "content": "The present investigation was undertaken in order to study the role played by the thyroid and the parathyroid glands in the development of osteoporosis induced by immobilization. One hundred and fifty-three male adult Sprague-Dawley rats were divided into 2 groups. The animals of one group were thyroparathyroidectomized; those of the other group were left intact. Each group was then divided into two subgroups in one of which the right hind leg of the animals were immobilized by elastic adhesive bandages. The animals were observed for varying periods of time up to 16 weeks. Bone mass and composition was determined and the results indicated that disuse osteoporosis occurs in rats in the virtual absence of the thyroid and parathyroid glands. The bone loss of the immobilized femur and tibia is however less pronounced in thyroparathyroidectomized than in intact rats.", "contents": "The effect of thyroparathyroidectomy: development of disuse osteoporosis in adult rats. The present investigation was undertaken in order to study the role played by the thyroid and the parathyroid glands in the development of osteoporosis induced by immobilization. One hundred and fifty-three male adult Sprague-Dawley rats were divided into 2 groups. The animals of one group were thyroparathyroidectomized; those of the other group were left intact. Each group was then divided into two subgroups in one of which the right hind leg of the animals were immobilized by elastic adhesive bandages. The animals were observed for varying periods of time up to 16 weeks. Bone mass and composition was determined and the results indicated that disuse osteoporosis occurs in rats in the virtual absence of the thyroid and parathyroid glands. The bone loss of the immobilized femur and tibia is however less pronounced in thyroparathyroidectomized than in intact rats.", "PMID": 954283}
{"id": "pubmed23n0032_1041", "title": "Calcitonin and the bone fluid compartment: effect of calcitonin and/or parathyroid hormone on plasma radiocalcium changes.", "content": "Changes in plasma calcium and 45Ca concentrations were followed after injection of calcitonin or a combination of calcitonin (CT) and parathyroid hormone (PTH) into thyroparathyroidectomized rats maintained on thyroxine. Comparison was made between rats injected with the hormone(s) after a recent feeding and after an overnight fast. Also cor (18 hr 45Ca) before, and more than 6 days (greater than 6 day 45Ca) prior to hormone administration. The following results were obtained: The action of CT predominated over that of PTH for the first few hours after injection. However, the effects of PTH were eventually manifested even when additional CT was administered. In the \"greater than 6 day\" 45Ca groups, PTH normally produced an increase in plasma 45Ca specific activity. However, in fasted rats, plasms 45Ca fell with total calcium with no change in specific activity following CT injection. In fed rats CT injection was followed by a decrease in plasma 45Ca specific activity. When both hormones were administered plasma 45Ca specific activity changes mimicked those produced by CT alone even after PTH plasma effects were manifested. In the \"18 hr\" 45Ca groups, CT produced first a drop in plasma 45Ca, followed by a reduction in the rate of its removal from plasma. It is concluded that the data can best be explained by the postulate that plasma calcium concentrations are maintained by the control of fluxes between bone fluid in the osteocyte-lining cell bone unit and the extracellular fluid. PTH increases the efflux from this bone fluid compartment while CT restricuts the source of calcium and, therefore, the efflux. Plasma 45Ca changes are due to a combination of changes in flux rates and mixing processes between the extracellular fluid compartment and the bone fluid compartment.", "contents": "Calcitonin and the bone fluid compartment: effect of calcitonin and/or parathyroid hormone on plasma radiocalcium changes. Changes in plasma calcium and 45Ca concentrations were followed after injection of calcitonin or a combination of calcitonin (CT) and parathyroid hormone (PTH) into thyroparathyroidectomized rats maintained on thyroxine. Comparison was made between rats injected with the hormone(s) after a recent feeding and after an overnight fast. Also cor (18 hr 45Ca) before, and more than 6 days (greater than 6 day 45Ca) prior to hormone administration. The following results were obtained: The action of CT predominated over that of PTH for the first few hours after injection. However, the effects of PTH were eventually manifested even when additional CT was administered. In the \"greater than 6 day\" 45Ca groups, PTH normally produced an increase in plasma 45Ca specific activity. However, in fasted rats, plasms 45Ca fell with total calcium with no change in specific activity following CT injection. In fed rats CT injection was followed by a decrease in plasma 45Ca specific activity. When both hormones were administered plasma 45Ca specific activity changes mimicked those produced by CT alone even after PTH plasma effects were manifested. In the \"18 hr\" 45Ca groups, CT produced first a drop in plasma 45Ca, followed by a reduction in the rate of its removal from plasma. It is concluded that the data can best be explained by the postulate that plasma calcium concentrations are maintained by the control of fluxes between bone fluid in the osteocyte-lining cell bone unit and the extracellular fluid. PTH increases the efflux from this bone fluid compartment while CT restricuts the source of calcium and, therefore, the efflux. Plasma 45Ca changes are due to a combination of changes in flux rates and mixing processes between the extracellular fluid compartment and the bone fluid compartment.", "PMID": 954281}
{"id": "pubmed23n0032_1042", "title": "Anterior tibial compartment syndrome associated with ergotamine ingestion.", "content": "This is the first reported case of acute anterior tibial compartment syndrome following ergotamine tartrate overdosage. A 25-year-old man developed signs and symptoms of ergotism after ingestion of 72 mg of ergotamine tartrate over the 12 days prior to his hospital admission. On the fourth hospital day, the patient developed bilateral anterior tibial compartment syndrome. The development of this syndrome is postulated to have been due to the progressive edema and tissue pressure in the anterior tibial compartments, worsened by the gradual improvement of the peripheral circulation which followed the diminished vasopastic effect of the ergotamine.", "contents": "Anterior tibial compartment syndrome associated with ergotamine ingestion. This is the first reported case of acute anterior tibial compartment syndrome following ergotamine tartrate overdosage. A 25-year-old man developed signs and symptoms of ergotism after ingestion of 72 mg of ergotamine tartrate over the 12 days prior to his hospital admission. On the fourth hospital day, the patient developed bilateral anterior tibial compartment syndrome. The development of this syndrome is postulated to have been due to the progressive edema and tissue pressure in the anterior tibial compartments, worsened by the gradual improvement of the peripheral circulation which followed the diminished vasopastic effect of the ergotamine.", "PMID": 954289}
{"id": "pubmed23n0032_1043", "title": "Fractures and dislocations involving the navicular-lunate axis.", "content": "This is a report of experience with 48 carpal disruptions including anterior dislocations of the lunate, transnavicular perilunate and perilunate dislocations and subluxation of the navicular. The correct radiographic diagnosis is absolutely essential for effective treatment. Many of these injuries may be treated by closed reduction, but open reduction of transnavicular perilunate dislocations is the procedure of choice. Avascular necrosis of the lunate may follow open dislocation. Primary subluxation of the navicular may occur without dislocation of the lunate.", "contents": "Fractures and dislocations involving the navicular-lunate axis. This is a report of experience with 48 carpal disruptions including anterior dislocations of the lunate, transnavicular perilunate and perilunate dislocations and subluxation of the navicular. The correct radiographic diagnosis is absolutely essential for effective treatment. Many of these injuries may be treated by closed reduction, but open reduction of transnavicular perilunate dislocations is the procedure of choice. Avascular necrosis of the lunate may follow open dislocation. Primary subluxation of the navicular may occur without dislocation of the lunate.", "PMID": 954290}
{"id": "pubmed23n0032_1044", "title": "Reconstruction of the posterior cruciate ligament: preliminary report of a new procedure.", "content": "The integrity of the posterior cruciate ligament is necessary for the stability of the knee. The popliteus tendon is a promising, practical, and anatomical donor for reconstructing the posterior cruciate ligament. The procedure can be performed relatively easily using a posterolateral Henderson incision in association with the standard anteromedial parapatellar approach. We have performed the operation in 2 patients, produced good results at 4 and 6 month follow-up examination.", "contents": "Reconstruction of the posterior cruciate ligament: preliminary report of a new procedure. The integrity of the posterior cruciate ligament is necessary for the stability of the knee. The popliteus tendon is a promising, practical, and anatomical donor for reconstructing the posterior cruciate ligament. The procedure can be performed relatively easily using a posterolateral Henderson incision in association with the standard anteromedial parapatellar approach. We have performed the operation in 2 patients, produced good results at 4 and 6 month follow-up examination.", "PMID": 954287}
{"id": "pubmed23n0032_1045", "title": "End result observations of X plate compression fixation. A critical review and analysis and a discussion of unsolved problems.", "content": "The X plate method of treatment of a diaphyseal fracture employs te internal, cortical and extramedullary compression-fixation of a shaft fracture, using a steel plate device of two components. Four wedges of the X device force the surfaces of the fracture against each other at the same time as the plate is pressed against the cortical surfaces of the reduced fracture fragments. The two components of the device are finally made to interlock firmly. Screws are not used. Plate onlay force and fracture compression force are created by the purchasing of the wedges against the rims of exactly predrilled cortical bores of the fracture fragments. A drill guide, and a device that permits pre-compression and temporary fixation of the fracture fragments are used when drilling is performed. Successvie designs of the employed X devices and auxiliary instruments are described. Clinical experience of the method so far has been satisfactory. Twenty-six forearm shaft fractures and two osteotomies of malunited shaft fractures have been successfuly treated. Present problems are related to the possible need of an implant material of a more springy character tan that of the 2353 (316L) steel implant, used at present and also to reduce the manufacturing costs.", "contents": "End result observations of X plate compression fixation. A critical review and analysis and a discussion of unsolved problems. The X plate method of treatment of a diaphyseal fracture employs te internal, cortical and extramedullary compression-fixation of a shaft fracture, using a steel plate device of two components. Four wedges of the X device force the surfaces of the fracture against each other at the same time as the plate is pressed against the cortical surfaces of the reduced fracture fragments. The two components of the device are finally made to interlock firmly. Screws are not used. Plate onlay force and fracture compression force are created by the purchasing of the wedges against the rims of exactly predrilled cortical bores of the fracture fragments. A drill guide, and a device that permits pre-compression and temporary fixation of the fracture fragments are used when drilling is performed. Successvie designs of the employed X devices and auxiliary instruments are described. Clinical experience of the method so far has been satisfactory. Twenty-six forearm shaft fractures and two osteotomies of malunited shaft fractures have been successfuly treated. Present problems are related to the possible need of an implant material of a more springy character tan that of the 2353 (316L) steel implant, used at present and also to reduce the manufacturing costs.", "PMID": 954291}
{"id": "pubmed23n0032_1046", "title": "Fatigue fractures of tibial and femoral shaft in soldiers.", "content": "In 136 fatigue fractures in military recruits, 58 were in the femoral shaft and 43 were in the tibia. Fatigue fractures in the long bones of the lower limb can be classified on the extend of the periosteal reaction. Most were grade 1, showing periosteal reaction only on one or two cortices as seen on the anteroposterior and lateral projections of the roentgenograms. Physical activity was discontinued immediately when fatigue fracture of the femur or tibia was suspected on clinical grounds alone. Early clinical diagnosis the long bone fatigue fractures in all patients except one. The injured soldiers were ambulatory without plaster cast immobilization or crutches. The suggested classification was used to select patients with Grade 1 fatigue fracture for early resumption of active military training.", "contents": "Fatigue fractures of tibial and femoral shaft in soldiers. In 136 fatigue fractures in military recruits, 58 were in the femoral shaft and 43 were in the tibia. Fatigue fractures in the long bones of the lower limb can be classified on the extend of the periosteal reaction. Most were grade 1, showing periosteal reaction only on one or two cortices as seen on the anteroposterior and lateral projections of the roentgenograms. Physical activity was discontinued immediately when fatigue fracture of the femur or tibia was suspected on clinical grounds alone. Early clinical diagnosis the long bone fatigue fractures in all patients except one. The injured soldiers were ambulatory without plaster cast immobilization or crutches. The suggested classification was used to select patients with Grade 1 fatigue fracture for early resumption of active military training.", "PMID": 954288}
{"id": "pubmed23n0032_1047", "title": "Lateral oblique incision for the Charnley low friction arthroplasty.", "content": "An oblique incision of the lateral exposure improves access to the acetabulum.", "contents": "Lateral oblique incision for the Charnley low friction arthroplasty. An oblique incision of the lateral exposure improves access to the acetabulum.", "PMID": 954293}
{"id": "pubmed23n0032_1048", "title": "Tibial rotational osteotomy.", "content": "Accurate rotational osteotomy is especially difficult in a triangular bone such as the tibia. A technique using a fibular graft to locate the center of rotation and provide stability has been used in 9 operations with very satisfying results. Average healing time was 7 1/2 weeks. A protractor has been designed to measure the degrees of rotation.", "contents": "Tibial rotational osteotomy. Accurate rotational osteotomy is especially difficult in a triangular bone such as the tibia. A technique using a fibular graft to locate the center of rotation and provide stability has been used in 9 operations with very satisfying results. Average healing time was 7 1/2 weeks. A protractor has been designed to measure the degrees of rotation.", "PMID": 954294}
{"id": "pubmed23n0032_1049", "title": "Clinical test for anterolateral rotary instability of the knee.", "content": "A simplified test for anterolateral rotary subluxation of the knee is largely based on the concepts described by Galway and McIntosh although performed in a different manner. The examiner places the right hand gently on the lateral side of the knee with the thumb overlying the posterior aspect of the fibula and the index finger palpating the anterolateral aspect of the joint line to determine the the tibiofemoral relationship. The left hand embraces the lateral side of the distal end of the femur with the thumb over the posterior aspect of the lateral femoral condyle. With equal pressure on the lateral femoral condyle and fibular head the knee is pushed gently forward into flexion. When anterointernal tibial luxation is present, a reduction phenomenon is felt as the knee passes into the 25 to 40 degree flexion range. This may occur as a sudden palpable and occasionally audible repositioning which is responsible for  such terminology as a \"pivot shift\" or \"jerk sign.\" We have experienced many instances where the reduction phenomenon is more subtle and is determined by palpation alone. The pathologic mechanics are determined by observations at surgery in 45 patients with a positive test. This test has improved our diagnositc ability and is easly taught to those unfamiliar with knee joint disorders.", "contents": "Clinical test for anterolateral rotary instability of the knee. A simplified test for anterolateral rotary subluxation of the knee is largely based on the concepts described by Galway and McIntosh although performed in a different manner. The examiner places the right hand gently on the lateral side of the knee with the thumb overlying the posterior aspect of the fibula and the index finger palpating the anterolateral aspect of the joint line to determine the the tibiofemoral relationship. The left hand embraces the lateral side of the distal end of the femur with the thumb over the posterior aspect of the lateral femoral condyle. With equal pressure on the lateral femoral condyle and fibular head the knee is pushed gently forward into flexion. When anterointernal tibial luxation is present, a reduction phenomenon is felt as the knee passes into the 25 to 40 degree flexion range. This may occur as a sudden palpable and occasionally audible repositioning which is responsible for  such terminology as a \"pivot shift\" or \"jerk sign.\" We have experienced many instances where the reduction phenomenon is more subtle and is determined by palpation alone. The pathologic mechanics are determined by observations at surgery in 45 patients with a positive test. This test has improved our diagnositc ability and is easly taught to those unfamiliar with knee joint disorders.", "PMID": 954292}
{"id": "pubmed23n0032_1050", "title": "Pathology of the limbus in untreated teratologic congenital dislocation of the hip. A case report of a ten-month-old- infant.", "content": "A dislocated hip in a 10-month-old infant with multiple congenital anomalies was examined postmortem. The true acetabulum was filled with areolar tissue and the acetabular articular cartilage was covered by fibrous pannus. The secondary acetabulum consisted of fibrous capsular tissue lined by synovial cells. A lip of new fibrous tissue, the limbus, grew over the cartilaginous labrum of the true acetabulum in juxtaposition to the dislocated femoral head.", "contents": "Pathology of the limbus in untreated teratologic congenital dislocation of the hip. A case report of a ten-month-old- infant. A dislocated hip in a 10-month-old infant with multiple congenital anomalies was examined postmortem. The true acetabulum was filled with areolar tissue and the acetabular articular cartilage was covered by fibrous pannus. The secondary acetabulum consisted of fibrous capsular tissue lined by synovial cells. A lip of new fibrous tissue, the limbus, grew over the cartilaginous labrum of the true acetabulum in juxtaposition to the dislocated femoral head.", "PMID": 954298}
{"id": "pubmed23n0032_1051", "title": "Dynamic fibular function: a new concept.", "content": "Fibular function has long been a point of controversy in the literature. Until recently the fibula's role during gait has been thought of as entirely static. A review of the literature reveals an important dynamic function for the fibula in maintaining ankle mortise stability during weight bearing. Roentgenographic and anatomical studies confirm the hypothesis of progressive distal fibular descent. A taut interosseous membrane and deepened mortise result when be flexors of the foot contract during stance phase. Congenital deformities of the ankle may stem from aberration in the dynamic role of the fibula. Stress fractures and problems resulting from impaired distal migration and synostosis formation are interpreted as disorders of the dynamic role of the fibula.", "contents": "Dynamic fibular function: a new concept. Fibular function has long been a point of controversy in the literature. Until recently the fibula's role during gait has been thought of as entirely static. A review of the literature reveals an important dynamic function for the fibula in maintaining ankle mortise stability during weight bearing. Roentgenographic and anatomical studies confirm the hypothesis of progressive distal fibular descent. A taut interosseous membrane and deepened mortise result when be flexors of the foot contract during stance phase. Congenital deformities of the ankle may stem from aberration in the dynamic role of the fibula. Stress fractures and problems resulting from impaired distal migration and synostosis formation are interpreted as disorders of the dynamic role of the fibula.", "PMID": 954295}
{"id": "pubmed23n0032_1052", "title": "Morphology of untreated bilateral congenital dislocation of the hips in a seventy-four-year-old man.", "content": "Two untreated completely dislocated hips were examined pathologically in a 74-year-old male dissecting room cadaver. The femoral heads bilaterally were 9 cm above the acetabular fossae. They lay adjacent to each ilium with no secondary acetabulum formation. A thickened and markedly elongated fibrous capsule appeared to be the major structure that had checked further migration of the femoral heads during weight-bearing. The femoral heads, although slightly flattened medially and somewhat smaller in size than in the normal adult, showed no degenerative arthritis. Dysplastic acetabular fossae were filled with soft tissue. Bone-to-bone contact with secondary degenerative changes was present only where the femurs, at the level of the lesser trochanters, rubbed against the overhanging superior acetabular rims.", "contents": "Morphology of untreated bilateral congenital dislocation of the hips in a seventy-four-year-old man. Two untreated completely dislocated hips were examined pathologically in a 74-year-old male dissecting room cadaver. The femoral heads bilaterally were 9 cm above the acetabular fossae. They lay adjacent to each ilium with no secondary acetabulum formation. A thickened and markedly elongated fibrous capsule appeared to be the major structure that had checked further migration of the femoral heads during weight-bearing. The femoral heads, although slightly flattened medially and somewhat smaller in size than in the normal adult, showed no degenerative arthritis. Dysplastic acetabular fossae were filled with soft tissue. Bone-to-bone contact with secondary degenerative changes was present only where the femurs, at the level of the lesser trochanters, rubbed against the overhanging superior acetabular rims.", "PMID": 954300}
{"id": "pubmed23n0032_1053", "title": "Congenital kyphoscoliosis with paralysis following hemivertebra excision.", "content": "This is a case report of a 13-year-old girl with a severely progressive congenital kyphoscoliosis, treated by halo-femoral traction and a single stage anterior and posterior hemivertebra excision. Forty-eight hours after surgery she developed paralysis of bowel, bladder and all motor function with partial preservation of deep sensation only. She began to have neurologic recovery 2 months later with gradual return of a completely normal neurological status over a period of 12 months. Pseudarthrosis and loss of correction of the original deformity required a second major anterior spine fusion 16 months after the initial procedure. No neurologic problems occurred following the second anterior fusion. A stable correction  was ultimately achieved. The mechanism of postoperative paralysis is unknown.", "contents": "Congenital kyphoscoliosis with paralysis following hemivertebra excision. This is a case report of a 13-year-old girl with a severely progressive congenital kyphoscoliosis, treated by halo-femoral traction and a single stage anterior and posterior hemivertebra excision. Forty-eight hours after surgery she developed paralysis of bowel, bladder and all motor function with partial preservation of deep sensation only. She began to have neurologic recovery 2 months later with gradual return of a completely normal neurological status over a period of 12 months. Pseudarthrosis and loss of correction of the original deformity required a second major anterior spine fusion 16 months after the initial procedure. No neurologic problems occurred following the second anterior fusion. A stable correction  was ultimately achieved. The mechanism of postoperative paralysis is unknown.", "PMID": 954301}
{"id": "pubmed23n0032_1054", "title": "Orthopedic manpower.", "content": "Key statistical information regarding Orthopedic Manpower is present to make orthopedists aware of the detailed information that is currently available. It is clear there is still insufficient data to justify radically altering the numbers or types of practices. Until the scope of orthopedic practice is more clearly defined and generally accepted (by orthopedists, other physicians, health professionals and the public) we must continue to monitor our activities and make adjustments through evolutionary changes rather than revolutionary regulations. Quite clearly there are segments of our country that are orthopedically underserved. Thus, it is vital that we seek new methods to encourage orthopedists to settle in these areas. Clearly, the simple production of more orthopedists in the traditional model will not answer the geographic maldistribution. With the tremendous scope of problems cared for by orthopedists, there does not appear to be an oversupply; however, we are headed in that direction. If we continue to rate operative procedures as the most significant and satisfying aspect of the practice, we certainly have enough, but they are not spread across the country in a uniform manner. Significant increases in numbers will be necessary if the orthopedist is to assume the role of general practitioner of the musculoskeletal system as well as a specialist for diagnosing and treating the more complicated musculoskeletal problems (whether by medical-surgical or physical-medical modalities). Should this be the case adjustments must be made in the selection process of orthopedic residents, in training programs, as well as estimates of the numbers of such specialists necessary for the future.", "contents": "Orthopedic manpower. Key statistical information regarding Orthopedic Manpower is present to make orthopedists aware of the detailed information that is currently available. It is clear there is still insufficient data to justify radically altering the numbers or types of practices. Until the scope of orthopedic practice is more clearly defined and generally accepted (by orthopedists, other physicians, health professionals and the public) we must continue to monitor our activities and make adjustments through evolutionary changes rather than revolutionary regulations. Quite clearly there are segments of our country that are orthopedically underserved. Thus, it is vital that we seek new methods to encourage orthopedists to settle in these areas. Clearly, the simple production of more orthopedists in the traditional model will not answer the geographic maldistribution. With the tremendous scope of problems cared for by orthopedists, there does not appear to be an oversupply; however, we are headed in that direction. If we continue to rate operative procedures as the most significant and satisfying aspect of the practice, we certainly have enough, but they are not spread across the country in a uniform manner. Significant increases in numbers will be necessary if the orthopedist is to assume the role of general practitioner of the musculoskeletal system as well as a specialist for diagnosing and treating the more complicated musculoskeletal problems (whether by medical-surgical or physical-medical modalities). Should this be the case adjustments must be made in the selection process of orthopedic residents, in training programs, as well as estimates of the numbers of such specialists necessary for the future.", "PMID": 954302}
{"id": "pubmed23n0032_1055", "title": "Cylindrical total ankle joint replacement: surgical and biomechanical rationale.", "content": "Incongruent designs of total ankle arthroplasty are all unsatisfactory owing to inherently poor wear, deformation resistance, and poor stability. Several congruent designs are capable of providing good function. It is concluded that a cylindrical surface with a horizontal axis located at the center of curvature of the lateral border of the talar dome seems most desirable where adequate subtalar motion is present and a spherical type seems most desirable where such motion is not present. The \"New Jersey\" cylindrical total ankle arthroplasty using a cylindrical surface uses an ultrahigh molecular weight polyethylene talar component and a mortised cobalt-chromium alloy tibial component both stabilized with methylmethacrylate bone cement and dual fixation fins.", "contents": "Cylindrical total ankle joint replacement: surgical and biomechanical rationale. Incongruent designs of total ankle arthroplasty are all unsatisfactory owing to inherently poor wear, deformation resistance, and poor stability. Several congruent designs are capable of providing good function. It is concluded that a cylindrical surface with a horizontal axis located at the center of curvature of the lateral border of the talar dome seems most desirable where adequate subtalar motion is present and a spherical type seems most desirable where such motion is not present. The \"New Jersey\" cylindrical total ankle arthroplasty using a cylindrical surface uses an ultrahigh molecular weight polyethylene talar component and a mortised cobalt-chromium alloy tibial component both stabilized with methylmethacrylate bone cement and dual fixation fins.", "PMID": 954296}
{"id": "pubmed23n0032_1056", "title": "Arthrodesis of the ankle joint with complete removal of the distal part of the fibula: experience with the transfibular approach and three different types of fixation.", "content": "In 20 patients the following 3 techniques have been used for ankle fusion, all with a lateral transfibular approach: (1) fibular grafting; (2) lateral removal of the distal fibula and fixation with 3 Blount staples; (3) removal of the distal fibular and fixation with Charnley compression clamps. The compression technique, through a lateral approach, was found to be superior as it avoids section of tendons. Removal of the distal portion of the fibular eliminates the risk of drainage and problems with wound healing due to the presence of a large bone graft immediately underneath the skin. Fusion is obtained more rapidly with the third than the first and second techniques. Pain seems to be related to osteoarthritis of the surrounding joints. The gait pattern depends not only on pain, but also on the position of the foot and on the posterior displacement of the talus. Less osteoarthritis as a result of shorter immoblizaition is the most favorable factor, notwithstanding the longer preoperative period in the third than in the first two methods.", "contents": "Arthrodesis of the ankle joint with complete removal of the distal part of the fibula: experience with the transfibular approach and three different types of fixation. In 20 patients the following 3 techniques have been used for ankle fusion, all with a lateral transfibular approach: (1) fibular grafting; (2) lateral removal of the distal fibula and fixation with 3 Blount staples; (3) removal of the distal fibular and fixation with Charnley compression clamps. The compression technique, through a lateral approach, was found to be superior as it avoids section of tendons. Removal of the distal portion of the fibular eliminates the risk of drainage and problems with wound healing due to the presence of a large bone graft immediately underneath the skin. Fusion is obtained more rapidly with the third than the first and second techniques. Pain seems to be related to osteoarthritis of the surrounding joints. The gait pattern depends not only on pain, but also on the position of the foot and on the posterior displacement of the talus. Less osteoarthritis as a result of shorter immoblizaition is the most favorable factor, notwithstanding the longer preoperative period in the third than in the first two methods.", "PMID": 954297}
{"id": "pubmed23n0032_1057", "title": "Solitary bone cyst of the cervical spine.", "content": "A histologically proven case of solitary bone cyst of a cervical vertebral body seems not to have been previously reported. Cysts of sufficient size to be a potential source of pathologic fracture should be excised and filled with autologous bone grafts.", "contents": "Solitary bone cyst of the cervical spine. A histologically proven case of solitary bone cyst of a cervical vertebral body seems not to have been previously reported. Cysts of sufficient size to be a potential source of pathologic fracture should be excised and filled with autologous bone grafts.", "PMID": 954303}
{"id": "pubmed23n0032_1058", "title": "Late instability in cervical spine fractures secondary to laminectomy.", "content": "Of 256 patients with a major spinal cord injury as a result of fracture of the cervical spine, 38 per cent had a laminectomy. Three months after injury, 33 per cent of the patients with laminectomy  required fusion for instability compared to 22 per cent of the nonlaminectomy group. There were no cases of late instability in pure flexion or extension fracture groups. Ninety per cent of the late instability cases were in the groups with hyperflexion and flexion compression fractures. Laminectomy should be avoided for these fractures, but early fusion may be necessary to prevent progressive deformity.", "contents": "Late instability in cervical spine fractures secondary to laminectomy. Of 256 patients with a major spinal cord injury as a result of fracture of the cervical spine, 38 per cent had a laminectomy. Three months after injury, 33 per cent of the patients with laminectomy  required fusion for instability compared to 22 per cent of the nonlaminectomy group. There were no cases of late instability in pure flexion or extension fracture groups. Ninety per cent of the late instability cases were in the groups with hyperflexion and flexion compression fractures. Laminectomy should be avoided for these fractures, but early fusion may be necessary to prevent progressive deformity.", "PMID": 954304}
{"id": "pubmed23n0032_1059", "title": "Spinal tuberculosis treated by antituberculous chemotherapy and radical operation.", "content": "Between 1956 and 1968, 208 patients with spinal tuberculosis healed by chemotherapy with and without surgical intervention. Two vertebral bodies were affected in 82 patients, one in 23 cases, more than 4 in 70 patients. Sixty-one patients had neural involvement at the time of admission, 42 of them an incomplete or complete paraplegia. All patients were treated by triple-drug chemotherapy. Chemotherapy and bed rest alone was sufficient for 76 patients. Surgical intervention was indicated in 132 cases. Debridement was carried out in 33 cases, debridement and spinal fusion was performed in 18 cases, anterolateral decompression of the spinal cord alone was employed in 15 cases, and together with spinal fusion recommended in 15 cases. Minor surgical procedures were made necessary in 8 cases. The indications for surgery were: (1) unfavorable response to conservative treatment during 3 months, (2) Pott's paraplegia showing no signs of recovery by conservative treatment, (3) Pott's paraplegia developing during conservative treatment (4) cases with unstable spinal lesions, and (5) cases with paravertebral abscesses and sinuses. Complete or good recovery occurred in 61 out of 76 conservatively treated patients. Forty-two out of 61 patients with neural involvement made a complete or a good recovery, 31 being patients with paraplegia. No improvement was noted in 16 cases with neural involvement, and 2 had progression of the neurological signs. A complete recovery was noted in 101 out of 132 operatively treated patients, a good recovery in 21, and no improvement in 9 patients with paraplegia. One patient died; 141 patients returned to their former work, 30 of whom were recovered paraplegics. Fifteen had retired because of advanced age. Modern treatment begins with triple-drug chemotherapy and bed rest for 3 months. If the response is unsatisfactory, debridement or debridement with spinal fusion is carried out as soon as possible. Anterolateral decompression is applied in cases with paraplegia.", "contents": "Spinal tuberculosis treated by antituberculous chemotherapy and radical operation. Between 1956 and 1968, 208 patients with spinal tuberculosis healed by chemotherapy with and without surgical intervention. Two vertebral bodies were affected in 82 patients, one in 23 cases, more than 4 in 70 patients. Sixty-one patients had neural involvement at the time of admission, 42 of them an incomplete or complete paraplegia. All patients were treated by triple-drug chemotherapy. Chemotherapy and bed rest alone was sufficient for 76 patients. Surgical intervention was indicated in 132 cases. Debridement was carried out in 33 cases, debridement and spinal fusion was performed in 18 cases, anterolateral decompression of the spinal cord alone was employed in 15 cases, and together with spinal fusion recommended in 15 cases. Minor surgical procedures were made necessary in 8 cases. The indications for surgery were: (1) unfavorable response to conservative treatment during 3 months, (2) Pott's paraplegia showing no signs of recovery by conservative treatment, (3) Pott's paraplegia developing during conservative treatment (4) cases with unstable spinal lesions, and (5) cases with paravertebral abscesses and sinuses. Complete or good recovery occurred in 61 out of 76 conservatively treated patients. Forty-two out of 61 patients with neural involvement made a complete or a good recovery, 31 being patients with paraplegia. No improvement was noted in 16 cases with neural involvement, and 2 had progression of the neurological signs. A complete recovery was noted in 101 out of 132 operatively treated patients, a good recovery in 21, and no improvement in 9 patients with paraplegia. One patient died; 141 patients returned to their former work, 30 of whom were recovered paraplegics. Fifteen had retired because of advanced age. Modern treatment begins with triple-drug chemotherapy and bed rest for 3 months. If the response is unsatisfactory, debridement or debridement with spinal fusion is carried out as soon as possible. Anterolateral decompression is applied in cases with paraplegia.", "PMID": 954305}
{"id": "pubmed23n0032_1060", "title": "Facetectomy for the relief of intraforaminal compression of the fifth lumbar root at the collapsed lumbosacral disk.", "content": "This paper presents a long-term follow-up of 23 patients with collapsed lumbosacral disks showing evidence of fifth lumbar root compression treated by facetectomy without fusion. Fifth lumbar root compression is usually caused by protrusion of the fourth lumbar disk. If this is not present the fifth lumbar root will almost invariably be found to be impinged upon laterally in the foramen by one of several mechanisms, which are detailed in this paper. Decompression of the roots at the fifth lumbar foramen may be done by either unilateral or bilateral facetectomy depending upon whether the symptoms are unilateral or bilateral. There has been no forward displacement with unilateral facetectomy and only slight forward movement of the fifth lumbar vertebrae on the sacrum with bilateral facetectomy. There has been good relief of symptoms in most patients. Arthrodesis, as a rule, is not necessary.", "contents": "Facetectomy for the relief of intraforaminal compression of the fifth lumbar root at the collapsed lumbosacral disk. This paper presents a long-term follow-up of 23 patients with collapsed lumbosacral disks showing evidence of fifth lumbar root compression treated by facetectomy without fusion. Fifth lumbar root compression is usually caused by protrusion of the fourth lumbar disk. If this is not present the fifth lumbar root will almost invariably be found to be impinged upon laterally in the foramen by one of several mechanisms, which are detailed in this paper. Decompression of the roots at the fifth lumbar foramen may be done by either unilateral or bilateral facetectomy depending upon whether the symptoms are unilateral or bilateral. There has been no forward displacement with unilateral facetectomy and only slight forward movement of the fifth lumbar vertebrae on the sacrum with bilateral facetectomy. There has been good relief of symptoms in most patients. Arthrodesis, as a rule, is not necessary.", "PMID": 954306}
{"id": "pubmed23n0032_1061", "title": "Congenital absence of the pectoral muscles. A review of twenty-five patients.", "content": "Twenty-five patients with unilateral partial absence of the pectoral major muscle were reviewed to determine the presence of fetal insult, associated anomalies, neoplasia, and prognosis. Fifteen had ipsilateral synbrachydactyly (the Poland Anomaly) which varied in severity from minimal findings to nearly complete absence of the hand. The pattern of abnormality was remarkably uniform with maximal involvement of the midhand and least involvement of the thumb and small finger. The etiology of this condition remains unknown although fetal insult is suggested in some cases. Although reports in the literature suggest a relationship with childhood leukemia, no malignancies were found in these children. None was found to have serious associated anomalies and all were judged to be of normal intelligence, suggesting a generally good prognosis.", "contents": "Congenital absence of the pectoral muscles. A review of twenty-five patients. Twenty-five patients with unilateral partial absence of the pectoral major muscle were reviewed to determine the presence of fetal insult, associated anomalies, neoplasia, and prognosis. Fifteen had ipsilateral synbrachydactyly (the Poland Anomaly) which varied in severity from minimal findings to nearly complete absence of the hand. The pattern of abnormality was remarkably uniform with maximal involvement of the midhand and least involvement of the thumb and small finger. The etiology of this condition remains unknown although fetal insult is suggested in some cases. Although reports in the literature suggest a relationship with childhood leukemia, no malignancies were found in these children. None was found to have serious associated anomalies and all were judged to be of normal intelligence, suggesting a generally good prognosis.", "PMID": 954307}
{"id": "pubmed23n0032_1062", "title": "Slipped capital femoral epiphysis. A study of 68 cases in the eastern half area of Japan.", "content": "Slipped capital femoral epiphysis is an uncommon disease among children in Japan. Eighty-one hips in 68 patients collected in the eastern half area of Japan were examined and studies in reference to the incidence, clinical characteristics, and results of treatment. The estimated yearly incidence rate in boys was 0.3 to 0.5 per 100,000 in the population between 10 and 14 years old. The higher incidence of boys (a 5.8 to 1 predilection for males) was characteristic. The disease was extremely rare in girls. Although Japanese children are generally neither as tall nor as heavy as Americans or Europeans, these patients were relatively taller and also much heavier than average. One of the reasons why children of Japan are not susceptible to slipped epiphysis may be explained by the Japanese physique. Satisfactory results were found in less than half the cases. Poor results were mainly caused by complications such as avascular necrosis and acute cartilage necrosis. The incidence of the latter complication was 20.7 per cent in 58 hips followed longer than a year and a half. The method of treatment seems not necessarily related to the development of chondrolysis. This complication is caused by an unknown genetic factor.", "contents": "Slipped capital femoral epiphysis. A study of 68 cases in the eastern half area of Japan. Slipped capital femoral epiphysis is an uncommon disease among children in Japan. Eighty-one hips in 68 patients collected in the eastern half area of Japan were examined and studies in reference to the incidence, clinical characteristics, and results of treatment. The estimated yearly incidence rate in boys was 0.3 to 0.5 per 100,000 in the population between 10 and 14 years old. The higher incidence of boys (a 5.8 to 1 predilection for males) was characteristic. The disease was extremely rare in girls. Although Japanese children are generally neither as tall nor as heavy as Americans or Europeans, these patients were relatively taller and also much heavier than average. One of the reasons why children of Japan are not susceptible to slipped epiphysis may be explained by the Japanese physique. Satisfactory results were found in less than half the cases. Poor results were mainly caused by complications such as avascular necrosis and acute cartilage necrosis. The incidence of the latter complication was 20.7 per cent in 58 hips followed longer than a year and a half. The method of treatment seems not necessarily related to the development of chondrolysis. This complication is caused by an unknown genetic factor.", "PMID": 954308}
{"id": "pubmed23n0032_1063", "title": "Congenital dislocation of the patella.", "content": "In 8 congenital patellar dislocations, in 7 patients, the patella was permanently and laterally dislocated, and irreducible or difficult to reduce. The patella usually was hypoplastic and showed absent facets. The intercondylar groove of the femur also was underdeveloped. In all but one patient, knee function was improved by a surgical procudure which involved lateral release of ptella, medial transpositon of patellar tendon, and plication of medial capsule or advancement of vastus medialis.", "contents": "Congenital dislocation of the patella. In 8 congenital patellar dislocations, in 7 patients, the patella was permanently and laterally dislocated, and irreducible or difficult to reduce. The patella usually was hypoplastic and showed absent facets. The intercondylar groove of the femur also was underdeveloped. In all but one patient, knee function was improved by a surgical procudure which involved lateral release of ptella, medial transpositon of patellar tendon, and plication of medial capsule or advancement of vastus medialis.", "PMID": 954309}
{"id": "pubmed23n0032_1064", "title": "Cast brace treatment of plateau and bicondylar fractures of the proximal tibia.", "content": "Twenty-nine patients with 30 intraarticular proximal tibial fractures were treated with early application of a cast brace. All fractures united, knee motion was excellent or good in all but one patient. The fracture fragments did not lose position after cast brace application. Varus or valgus deformities could be corrected and maintained. Ninety per cent had no pain at follow-up. Hospital stay was as short as two days and generally under two weeks.", "contents": "Cast brace treatment of plateau and bicondylar fractures of the proximal tibia. Twenty-nine patients with 30 intraarticular proximal tibial fractures were treated with early application of a cast brace. All fractures united, knee motion was excellent or good in all but one patient. The fracture fragments did not lose position after cast brace application. Varus or valgus deformities could be corrected and maintained. Ninety per cent had no pain at follow-up. Hospital stay was as short as two days and generally under two weeks.", "PMID": 954310}
{"id": "pubmed23n0032_1065", "title": "The normal carrying angle of the elbow. A radiographic study of 422 patients.", "content": "A radiographic study of the carrying angle in 422 patients demonstrated that the carrying angle increased with age, but there were no real differences in males and females. Apparent differences may be explained by increased joint laxity in females, permitting a greater degree of extension.", "contents": "The normal carrying angle of the elbow. A radiographic study of 422 patients. A radiographic study of the carrying angle in 422 patients demonstrated that the carrying angle increased with age, but there were no real differences in males and females. Apparent differences may be explained by increased joint laxity in females, permitting a greater degree of extension.", "PMID": 954311}
{"id": "pubmed23n0032_1066", "title": "Screw fixation of the olecranon after fracture or osteotomy.", "content": "For 10 years prior to the availability of the Olecranon Screw described in this article, oblique fixation using a regular Sherman bone screw was employed for displaced fractures (with a suitable fragment) by engaging the anterior ulnar cortex. Internal fixation was supplemented by a split for 3 weeks. The reason for a special olecranon screw is to eliminate the necessity for additional fixation and permit early motion. No instance of non-union or important restriction of motion or serious complication has been encountered.", "contents": "Screw fixation of the olecranon after fracture or osteotomy. For 10 years prior to the availability of the Olecranon Screw described in this article, oblique fixation using a regular Sherman bone screw was employed for displaced fractures (with a suitable fragment) by engaging the anterior ulnar cortex. Internal fixation was supplemented by a split for 3 weeks. The reason for a special olecranon screw is to eliminate the necessity for additional fixation and permit early motion. No instance of non-union or important restriction of motion or serious complication has been encountered.", "PMID": 954312}
{"id": "pubmed23n0032_1067", "title": "Clinical features and treatment of joint dislocations in Larsen's syndrome. Report of three cases in one family.", "content": "Of 3 cases of Larsen's syndrome in one family, one had bilateral dislocation of the hip and the knee joints. The knee joint was not reduced by manipulation and corrective cast, while both hip and knee joints were simultaneously reduced by skeletal traction of the tibia at the age of 4 months, and the course was satisfactory. Reduction of joint dislocations should be attempted by a conservative method such as skeletal traction as early as possible. Various radiographic skeletal abnormalities occurred in all 3 cases. Larsen's syndrome very likely has a genetic origin.", "contents": "Clinical features and treatment of joint dislocations in Larsen's syndrome. Report of three cases in one family. Of 3 cases of Larsen's syndrome in one family, one had bilateral dislocation of the hip and the knee joints. The knee joint was not reduced by manipulation and corrective cast, while both hip and knee joints were simultaneously reduced by skeletal traction of the tibia at the age of 4 months, and the course was satisfactory. Reduction of joint dislocations should be attempted by a conservative method such as skeletal traction as early as possible. Various radiographic skeletal abnormalities occurred in all 3 cases. Larsen's syndrome very likely has a genetic origin.", "PMID": 954313}
{"id": "pubmed23n0032_1068", "title": "Osteogenetic epithelial-mesenchymal cell interactions.", "content": "FL, HeLa and other non-murine epithelial cells are capable of inducing chondroosseous differentiation when brought into contact with mouse thigh mesenchymal cells. Immunosuppression as produced by cortisone or mouse antilymphocyte serum is required to prevent rejection and to allow growth of the foreign epithelial cells. Cartilage appears within 7 days and bone within 9 days following epithelial cell injection. Electron microscopic studies indicate that the induced cartilage and bone is normal in basic strucutre, and that calcification is mediated by extracellular matrix vesicles. Various forms of mechanical and chemical injury have consistently failed to stimulate cartilage or bone formation in the mouse thigh. Explantation of preosseous thigh tumors to mouse brain allows continued growth of human and mouse cells with development of cartilage or bone in 67 per cent of implants. Microscopy indicates cartilage and bone formation by many mesenchymal cells which are not in direct contact with epithelium. To account for this, two mechanisms of bone induction are envisioned: (1) short-range diffusion of inductive substance(s) and/or (2) transfer of the inductive stimulus by direct surface contact from epithelial cell to mesenchymal cell to mesenchymal cell and so on.", "contents": "Osteogenetic epithelial-mesenchymal cell interactions. FL, HeLa and other non-murine epithelial cells are capable of inducing chondroosseous differentiation when brought into contact with mouse thigh mesenchymal cells. Immunosuppression as produced by cortisone or mouse antilymphocyte serum is required to prevent rejection and to allow growth of the foreign epithelial cells. Cartilage appears within 7 days and bone within 9 days following epithelial cell injection. Electron microscopic studies indicate that the induced cartilage and bone is normal in basic strucutre, and that calcification is mediated by extracellular matrix vesicles. Various forms of mechanical and chemical injury have consistently failed to stimulate cartilage or bone formation in the mouse thigh. Explantation of preosseous thigh tumors to mouse brain allows continued growth of human and mouse cells with development of cartilage or bone in 67 per cent of implants. Microscopy indicates cartilage and bone formation by many mesenchymal cells which are not in direct contact with epithelium. To account for this, two mechanisms of bone induction are envisioned: (1) short-range diffusion of inductive substance(s) and/or (2) transfer of the inductive stimulus by direct surface contact from epithelial cell to mesenchymal cell to mesenchymal cell and so on.", "PMID": 954314}
{"id": "pubmed23n0032_1069", "title": "The effects of hyperoxia upon bone in organ culture.", "content": "Hyperoxia has been reported to stimulate both resorption and synthesis of bone in vitro. The effects of increased oxygen tension were re-investigated using calvaria from infant mice maintained in a stationary grid culture system for 48 48 hours with an unsupplemented chemically defined medium. Resting resorption due to osteoclastic activity was demonstrated in the explants in air by Von Kossa staining, histology, and 45 Ca release. Resorption was inhibited by exposure to 95 per cent oxygen or hyperbaric oxygenation at 2 atmospheres pressure. Hyperoxia also depressed new bone formatin by osteoblasts although the production of a new collagen, as measured by the incorporation of 3H-proline, was greater in calvaria cultured in hyperbaric oxygen than in paired explants in 95 per cent oxygen. Thus hyperoxia was toxic for both synthetic and resorptive activity of bone cells; these effects may stem from the loss of vital factors present in natural MEDIA supplements.", "contents": "The effects of hyperoxia upon bone in organ culture. Hyperoxia has been reported to stimulate both resorption and synthesis of bone in vitro. The effects of increased oxygen tension were re-investigated using calvaria from infant mice maintained in a stationary grid culture system for 48 48 hours with an unsupplemented chemically defined medium. Resting resorption due to osteoclastic activity was demonstrated in the explants in air by Von Kossa staining, histology, and 45 Ca release. Resorption was inhibited by exposure to 95 per cent oxygen or hyperbaric oxygenation at 2 atmospheres pressure. Hyperoxia also depressed new bone formatin by osteoblasts although the production of a new collagen, as measured by the incorporation of 3H-proline, was greater in calvaria cultured in hyperbaric oxygen than in paired explants in 95 per cent oxygen. Thus hyperoxia was toxic for both synthetic and resorptive activity of bone cells; these effects may stem from the loss of vital factors present in natural MEDIA supplements.", "PMID": 954315}
{"id": "pubmed23n0032_1070", "title": "The anatomy and pathology of congenital dislocation of the hip.", "content": "At least 1 per cent of all newborn infants in the United Kingdom have CDH (Fig 10). Among these infants, approximately 85 per cent are normally formed and 15 per cent malformed. Among the normally formed infants, 90 per cent of the CDH are of the Grade 1 type, and 10 per cent of Grades 2 and 3. Such infants have a perinatal mortality of about 5 per cent. In contrast, among the malformed infants, about 50 per cent are CDH Grade 1, and 50 per cent Grades 2 and 3; their perinatal mortality is around 70 per cent. In other words, a much higher proportion than usual of the severe grades of CDH are found among the infants with a high perinatal mortality.", "contents": "The anatomy and pathology of congenital dislocation of the hip. At least 1 per cent of all newborn infants in the United Kingdom have CDH (Fig 10). Among these infants, approximately 85 per cent are normally formed and 15 per cent malformed. Among the normally formed infants, 90 per cent of the CDH are of the Grade 1 type, and 10 per cent of Grades 2 and 3. Such infants have a perinatal mortality of about 5 per cent. In contrast, among the malformed infants, about 50 per cent are CDH Grade 1, and 50 per cent Grades 2 and 3; their perinatal mortality is around 70 per cent. In other words, a much higher proportion than usual of the severe grades of CDH are found among the infants with a high perinatal mortality.", "PMID": 954316}
{"id": "pubmed23n0032_1071", "title": "Pathological human synovial fluids. Viscosity and boundary lubricating properties.", "content": "On human synovial fluids obtained during operations from the knee joints of 80 patients with different joing disease, relative viscosity was measured at 3 different shear rates and the boundary lubrication was tested by the coefficient of friction in an artificial rubber/glass system. The results were evaluated in relation to the operative findings. The viscosity showed statistically significant differences between the synovial fluids from the knee joints with rheumatoid arthritis, osteoarthritis, and torn menisci, being lowest in rheumatoid synovial fluid and highest in synovial fluids from knee joints with torn menisci. Correlations were found in the variations in the viscosity and the degree of synovitis. The boundary lubrication also showed different values inrelation to the different diseases. Synovial fluid from knee joints with torn menisci seemed to act as the best lubricant and significantly better than rheumatoid synovial fluid. Variations in the boundary lubrications reflect successive degrees of cartilage degeneration.", "contents": "Pathological human synovial fluids. Viscosity and boundary lubricating properties. On human synovial fluids obtained during operations from the knee joints of 80 patients with different joing disease, relative viscosity was measured at 3 different shear rates and the boundary lubrication was tested by the coefficient of friction in an artificial rubber/glass system. The results were evaluated in relation to the operative findings. The viscosity showed statistically significant differences between the synovial fluids from the knee joints with rheumatoid arthritis, osteoarthritis, and torn menisci, being lowest in rheumatoid synovial fluid and highest in synovial fluids from knee joints with torn menisci. Correlations were found in the variations in the viscosity and the degree of synovitis. The boundary lubrication also showed different values inrelation to the different diseases. Synovial fluid from knee joints with torn menisci seemed to act as the best lubricant and significantly better than rheumatoid synovial fluid. Variations in the boundary lubrications reflect successive degrees of cartilage degeneration.", "PMID": 954317}
{"id": "pubmed23n0032_1072", "title": "Monomer leakage from polymerizing acrylic bone cement. An in vitro study on the influence of speed and duration of mixing, cement volume and surface area.", "content": "An in vitro study was dthesigned to measure the effects of variable cement volumes and modified mixing techniques on the monomer leakage after simulated implantation. The cement volume as such was found to be an irrelevant parameter for calculations of the monomer loss from bone cement. The leakage was directly related to the surface area of the cement. Variations in the thickness of the cement mantle from 1 to 5 mm did not affect the leakage. Late cement insertion should reduce the total monomer leakage into the tissues. It is doubtful whether an increased stirring speed during mixing reduces the monomer leakage after implantation.", "contents": "Monomer leakage from polymerizing acrylic bone cement. An in vitro study on the influence of speed and duration of mixing, cement volume and surface area. An in vitro study was dthesigned to measure the effects of variable cement volumes and modified mixing techniques on the monomer leakage after simulated implantation. The cement volume as such was found to be an irrelevant parameter for calculations of the monomer loss from bone cement. The leakage was directly related to the surface area of the cement. Variations in the thickness of the cement mantle from 1 to 5 mm did not affect the leakage. Late cement insertion should reduce the total monomer leakage into the tissues. It is doubtful whether an increased stirring speed during mixing reduces the monomer leakage after implantation.", "PMID": 954318}
{"id": "pubmed23n0032_1073", "title": "Effects of laminations and blood entrapment on the strength of acrylic bone cement.", "content": "The tensile and shear strengths of surgically mixed acrylic bone cement were measured for intact specimens and for special two-part cylindrical specimens containing single laminations perpendicular to their longitudinal axes. Laminations were formed at times ranging from 3 1/2 to 6 1/2 minutes after initial mixing of the powder and liquid for dry interfaces and for interfaces containing fresh blood at the time of lamination formation. In additon, special tests were conducted to measure the bond strength between freshly mixed and pre-polymerized acrylic. The tensile and shear strengths at the lamination interface decreased significantly when the laminations were formed late in the working life of the acrylic. The presence of blood at the interface further weakened the bond to approximately 25 per cent (tension) and 36 per cent (shear) of the virgin strengths of the material. These results support early acrylic placement with dry surgical fields. The bonding of fresh acrylic to pre-polymerized material was most effective when the material was poured into the mold before dough stage. This technique would have practical application in special cases for re-cementing revision prostheses without removing the old acrylic from within the bone.", "contents": "Effects of laminations and blood entrapment on the strength of acrylic bone cement. The tensile and shear strengths of surgically mixed acrylic bone cement were measured for intact specimens and for special two-part cylindrical specimens containing single laminations perpendicular to their longitudinal axes. Laminations were formed at times ranging from 3 1/2 to 6 1/2 minutes after initial mixing of the powder and liquid for dry interfaces and for interfaces containing fresh blood at the time of lamination formation. In additon, special tests were conducted to measure the bond strength between freshly mixed and pre-polymerized acrylic. The tensile and shear strengths at the lamination interface decreased significantly when the laminations were formed late in the working life of the acrylic. The presence of blood at the interface further weakened the bond to approximately 25 per cent (tension) and 36 per cent (shear) of the virgin strengths of the material. These results support early acrylic placement with dry surgical fields. The bonding of fresh acrylic to pre-polymerized material was most effective when the material was poured into the mold before dough stage. This technique would have practical application in special cases for re-cementing revision prostheses without removing the old acrylic from within the bone.", "PMID": 954319}
{"id": "pubmed23n0032_1074", "title": "Normal values of the hip joint for the evaluation of X-rays in children and adults.", "content": "X-ray measurements of the hip joints of children, with special reference to the acetabular index, suggest that the upper standard deviation of normal comprises the borderline to a critical zone where extreme values of normal and pathologic hips were found together. Above the double standard deviation only severe dysplasias were present. Investigations of the shaft-neck angle and the degree of anteversion including the wide standard deviation demonstrate that it is very difficult to determine where these angles become pathologic. It is more important to look for the relationship between femoral head and acetabulum. A new measurement--the Hip Value is based on measurements of the Idelberg- Frank angle, the Wiberg angle and MZ-distance of decentralization. By statistical methods, normal and pathological joints can be separated as follows: in adult Hip Values, between 6 and 15 indicate a normal joint form; values between 16 and 21 indicate a slight deformation and values of 22 and above are indications of a severe deformation, in children in the normal range the Hip Value reaches 14; values of 15 and up are pathological.", "contents": "Normal values of the hip joint for the evaluation of X-rays in children and adults. X-ray measurements of the hip joints of children, with special reference to the acetabular index, suggest that the upper standard deviation of normal comprises the borderline to a critical zone where extreme values of normal and pathologic hips were found together. Above the double standard deviation only severe dysplasias were present. Investigations of the shaft-neck angle and the degree of anteversion including the wide standard deviation demonstrate that it is very difficult to determine where these angles become pathologic. It is more important to look for the relationship between femoral head and acetabulum. A new measurement--the Hip Value is based on measurements of the Idelberg- Frank angle, the Wiberg angle and MZ-distance of decentralization. By statistical methods, normal and pathological joints can be separated as follows: in adult Hip Values, between 6 and 15 indicate a normal joint form; values between 16 and 21 indicate a slight deformation and values of 22 and above are indications of a severe deformation, in children in the normal range the Hip Value reaches 14; values of 15 and up are pathological.", "PMID": 954321}
{"id": "pubmed23n0032_1075", "title": "Intertrochanteric femoral osteotomy with concentric reduction of the femoral head in treatment of residual congenital acetabular dysplasia.", "content": "A method of classification based upon increase of the anteversion and valgus deformity of the upper femur associated with a dysplastic acetabulum and subluxation of the femoral head demonstrates 4 main groups: I or II in patients 1 to 3 years of age; III, patients approximately 2 years of age; IV, patients with absolute contraindication for surgical intervention. The Monticelli technique employs a Kirschner wire for the internal fixation and incorporates it in a hip spica. The results of the surgical treatment are very gratifying when classification and specified indications are correct. In follow-up observations ranging from 3 to 11 years, with an average of 7 years, there were practically no complications other than a few superficial skin infections.", "contents": "Intertrochanteric femoral osteotomy with concentric reduction of the femoral head in treatment of residual congenital acetabular dysplasia. A method of classification based upon increase of the anteversion and valgus deformity of the upper femur associated with a dysplastic acetabulum and subluxation of the femoral head demonstrates 4 main groups: I or II in patients 1 to 3 years of age; III, patients approximately 2 years of age; IV, patients with absolute contraindication for surgical intervention. The Monticelli technique employs a Kirschner wire for the internal fixation and incorporates it in a hip spica. The results of the surgical treatment are very gratifying when classification and specified indications are correct. In follow-up observations ranging from 3 to 11 years, with an average of 7 years, there were practically no complications other than a few superficial skin infections.", "PMID": 954322}
{"id": "pubmed23n0032_1076", "title": "The intertrochanteric osteotomy in the treatment of congenital dysplasia of the hip.", "content": "The dysplastic acetabulum develops to normal shape only if the head of the femur is in central position in the articualr cavity correct according to biomechanics of the hip joint. Anterior rotation of the neck of the femur should be reduced operatively to neutral position as soon as possible if joint congruity cannot be achieved by conservative treatment. The indication for an intertrochanteric derotation osteotomy is made in cases demonstrating a roof inclination less than 30 degrees in children age 1 1/2 to three. After the third year of age, the mutual growth-stimulating effect of the  articular bodies is not important enough to be useful in treatment of dysplasia. The femoral osteotomy has to be performed intertrochanterically. If articular congruity cannot be achieved by intertrochanteric osteotomy only, an additional innominate osteotomy of the pelvis is indicated at the same stage. The results of 435 cases demonstrated a good development of the acetabular roof if the intervention was performed early, according to correctly specified indications. In cases with insufficient growth potential of the acetabular roof, resulting in an increased inclination, an additional reconstruction of the acetabular cavity is required.", "contents": "The intertrochanteric osteotomy in the treatment of congenital dysplasia of the hip. The dysplastic acetabulum develops to normal shape only if the head of the femur is in central position in the articualr cavity correct according to biomechanics of the hip joint. Anterior rotation of the neck of the femur should be reduced operatively to neutral position as soon as possible if joint congruity cannot be achieved by conservative treatment. The indication for an intertrochanteric derotation osteotomy is made in cases demonstrating a roof inclination less than 30 degrees in children age 1 1/2 to three. After the third year of age, the mutual growth-stimulating effect of the  articular bodies is not important enough to be useful in treatment of dysplasia. The femoral osteotomy has to be performed intertrochanterically. If articular congruity cannot be achieved by intertrochanteric osteotomy only, an additional innominate osteotomy of the pelvis is indicated at the same stage. The results of 435 cases demonstrated a good development of the acetabular roof if the intervention was performed early, according to correctly specified indications. In cases with insufficient growth potential of the acetabular roof, resulting in an increased inclination, an additional reconstruction of the acetabular cavity is required.", "PMID": 954323}
{"id": "pubmed23n0032_1077", "title": "Congenital hip dysplasia in the light of early and very early diagnosis.", "content": "Ortolani summarizes 40 years of experience with about 8,000 children with congenital hip dysplasia, treated from birth or at a very early age. All newborns should be screened and examined for congenital hip dysplasia and this exam should be repeated at one month and again in 3 months. The most reliable sign for the diagnosis of congenital hip dysplasia at birth is the \"click\" sign. The etiology of congenital hip dysplasia is in a localized fault in embryonic development. The treatment started just as early as the condition was suspected. Surgical treatment is recommended for all complete congenital hip dislocations not amenable to treatment by closed methods.", "contents": "Congenital hip dysplasia in the light of early and very early diagnosis. Ortolani summarizes 40 years of experience with about 8,000 children with congenital hip dysplasia, treated from birth or at a very early age. All newborns should be screened and examined for congenital hip dysplasia and this exam should be repeated at one month and again in 3 months. The most reliable sign for the diagnosis of congenital hip dysplasia at birth is the \"click\" sign. The etiology of congenital hip dysplasia is in a localized fault in embryonic development. The treatment started just as early as the condition was suspected. Surgical treatment is recommended for all complete congenital hip dislocations not amenable to treatment by closed methods.", "PMID": 954324}
{"id": "pubmed23n0032_1078", "title": "Combined procedure of open reduction and shortening of the femur in treatment of congenital dislocation of the hips in older children.", "content": "A one-stage, combined operative procedure for reduction of congenitally dislocated hips in older children consists of shortening of the femur; open reduction by an inferior approach to the joint; reconstruction of the acetabular roof; correction of anteversion of both the femoral neck and the neck-shaft angle; anterior transposition of the iliopsoas muscle. On 60 hips operated in children, ages 5 to 15 with a follow-up period, ranging from 5 to 9 years, the results were found to be excellent in 3 per cent, good in 60 per cent, fair in 30 per cent, and poor in 7 per cent. The procedure is indicated in children up to the time of early puberty. The primary indication is high bilateral dislocation. In unilateral dislocations some residual leg-length discrepancy frequently occurs. This procedure should be done only by orthopedic surgeons who have special training and experience in the treatment of congenital dislocation of the hip.", "contents": "Combined procedure of open reduction and shortening of the femur in treatment of congenital dislocation of the hips in older children. A one-stage, combined operative procedure for reduction of congenitally dislocated hips in older children consists of shortening of the femur; open reduction by an inferior approach to the joint; reconstruction of the acetabular roof; correction of anteversion of both the femoral neck and the neck-shaft angle; anterior transposition of the iliopsoas muscle. On 60 hips operated in children, ages 5 to 15 with a follow-up period, ranging from 5 to 9 years, the results were found to be excellent in 3 per cent, good in 60 per cent, fair in 30 per cent, and poor in 7 per cent. The procedure is indicated in children up to the time of early puberty. The primary indication is high bilateral dislocation. In unilateral dislocations some residual leg-length discrepancy frequently occurs. This procedure should be done only by orthopedic surgeons who have special training and experience in the treatment of congenital dislocation of the hip.", "PMID": 954325}
{"id": "pubmed23n0032_1079", "title": "Treatment of congenital hip dislocation by muscle release, skeletal traction and closed reduction in older children.", "content": "A method of treatment of congenital hip dislocation by preliminary multiple muscle releases around the hip, followed by skeletal traction, is employed for closed reduction and immobilization with long leg cast. In 29 hips treated during the period from 1961 to 1972, the results justify the method for treatment of children 3 to 11 years of age with neglected high posterior congenital hip dislocations. The main prerequisite for the procedure is a fairly well-developed acetabulum.", "contents": "Treatment of congenital hip dislocation by muscle release, skeletal traction and closed reduction in older children. A method of treatment of congenital hip dislocation by preliminary multiple muscle releases around the hip, followed by skeletal traction, is employed for closed reduction and immobilization with long leg cast. In 29 hips treated during the period from 1961 to 1972, the results justify the method for treatment of children 3 to 11 years of age with neglected high posterior congenital hip dislocations. The main prerequisite for the procedure is a fairly well-developed acetabulum.", "PMID": 954326}
{"id": "pubmed23n0032_1080", "title": "An evaluation of conservative and operative methods in the treatment of congenital hip dislocation.", "content": "An evaluation of different methods of closed reduction of dislocated hips during different periods of time shows that manual reduction and fixation in Lorenz position account for a high rate of avascular necrosis. Even with functional and slow reduction by Pavlik harness and overhead extension, there is still a certain percentage of femoral capital necrosis. The fetal position recommended by Fettweis and Salter seems to reduce the incidence of necrosis. Acetabuloplasty combined with detorsion-varus-ostcotomy provides the means of normalizing a hip joint with a greater degree of success than most other methods.", "contents": "An evaluation of conservative and operative methods in the treatment of congenital hip dislocation. An evaluation of different methods of closed reduction of dislocated hips during different periods of time shows that manual reduction and fixation in Lorenz position account for a high rate of avascular necrosis. Even with functional and slow reduction by Pavlik harness and overhead extension, there is still a certain percentage of femoral capital necrosis. The fetal position recommended by Fettweis and Salter seems to reduce the incidence of necrosis. Acetabuloplasty combined with detorsion-varus-ostcotomy provides the means of normalizing a hip joint with a greater degree of success than most other methods.", "PMID": 954327}
{"id": "pubmed23n0032_1081", "title": "Overdiagnosis of congenital dislocation of the hip.", "content": "In a retrospective study of 548 cases of CDH diagnosed and treated immediately after birth in the city of Malmo, 90 per cent may not have developed manifest dislocation even without treatment when the expected incidence is taken into account. It could, however, be demonstrated from variables such as sex ratio, birth rank, fetal presentation and the size of the capital epiphyses that the entire group indeed had characteristics relating them to CDH. These characteristics were more obvious in coses with a firmly established diagnosis. Cases in whom the diagnosis could not be confirmed radiologically deviated less or not at all from the general population.", "contents": "Overdiagnosis of congenital dislocation of the hip. In a retrospective study of 548 cases of CDH diagnosed and treated immediately after birth in the city of Malmo, 90 per cent may not have developed manifest dislocation even without treatment when the expected incidence is taken into account. It could, however, be demonstrated from variables such as sex ratio, birth rank, fetal presentation and the size of the capital epiphyses that the entire group indeed had characteristics relating them to CDH. These characteristics were more obvious in coses with a firmly established diagnosis. Cases in whom the diagnosis could not be confirmed radiologically deviated less or not at all from the general population.", "PMID": 954328}
{"id": "pubmed23n0032_1082", "title": "Total avascular necrosis of the capital femoral epiphysis in congenital dislocated hips.", "content": "Ninety-four hips involved with total avascular necrosis in the treatment of congenital dislocation of the hips were reviewed in a search for etiological factors and effective salvage procedures. Pre-reduction traction and adductor tenotomy did not prevent avascular necrosis. Abduction of the hip in the \"frog\" position was the incriminating common denominator. This position may cause (1) interference of the blood supply to the femoral head by compression of the medial branch of the deep profundus artery; and (2) pressure on the intraepiphyseal grooves by the glenoid labrum. The containment of the femoral head by acetabuloplasty (preferably) below age 8, followed at a later date (over age 10) by transfer distally of the greater trochanter, with the abductor muscles, offers a satisfactory salvage procedure.", "contents": "Total avascular necrosis of the capital femoral epiphysis in congenital dislocated hips. Ninety-four hips involved with total avascular necrosis in the treatment of congenital dislocation of the hips were reviewed in a search for etiological factors and effective salvage procedures. Pre-reduction traction and adductor tenotomy did not prevent avascular necrosis. Abduction of the hip in the \"frog\" position was the incriminating common denominator. This position may cause (1) interference of the blood supply to the femoral head by compression of the medial branch of the deep profundus artery; and (2) pressure on the intraepiphyseal grooves by the glenoid labrum. The containment of the femoral head by acetabuloplasty (preferably) below age 8, followed at a later date (over age 10) by transfer distally of the greater trochanter, with the abductor muscles, offers a satisfactory salvage procedure.", "PMID": 954329}
{"id": "pubmed23n0032_1083", "title": "Acetabular growth potential in congenital dislocation of the hip and some factors upon which it may depend.", "content": "The factors which determine the rate and amount of acetabular growth in congenital dislocation of the hip are ill-understood. A prospective radiological study has been devised in order to assess the influence of the age of congruity, femoral anteversion and neck/shaft angles on acetabular growth. To be included, the children must have reached at least 8 years of age. Those with associated congenital abnormalities and subluxation were excluded, as were those who had operations for acetabular reconstruction. Seventy-four hips were suitable for analysis, and the age range at follow-up was from 8 to 17 years. The acetabular angle was used as an index of acetabular growth. Measurement of this angle, and the anteversion and neck/shaft angles were made before, and each year after reduction of the hip. Congruity was assessed from a radiograph with the legs in the functional position. A satisfactory acetabulum was obtained in 44 hips and the mean age of congruity was 33 months; the acetabulum was unsatisfactory in 30 hips, with a mean age of congruity of 48 months. Four years is the critical age, for if congruity is obtained later, the risk of producing a moderate or severely dysplastic acetabulum is more than doubled. If congruity is obtained under four years of age, growth of the acetabulum with continue in most patients up to 8 and in some to 11 YEARS OF age; the resulting acetabulum was normal or mildly dysplastic. Fifty-nine per cent of the satisfactory hips at follow-up had a normal anteversion angle, and 6 per cent a normal neck/shaft angle. Correction of these angles by themselves, seems not to be a major importance for promoting acetabular growth.", "contents": "Acetabular growth potential in congenital dislocation of the hip and some factors upon which it may depend. The factors which determine the rate and amount of acetabular growth in congenital dislocation of the hip are ill-understood. A prospective radiological study has been devised in order to assess the influence of the age of congruity, femoral anteversion and neck/shaft angles on acetabular growth. To be included, the children must have reached at least 8 years of age. Those with associated congenital abnormalities and subluxation were excluded, as were those who had operations for acetabular reconstruction. Seventy-four hips were suitable for analysis, and the age range at follow-up was from 8 to 17 years. The acetabular angle was used as an index of acetabular growth. Measurement of this angle, and the anteversion and neck/shaft angles were made before, and each year after reduction of the hip. Congruity was assessed from a radiograph with the legs in the functional position. A satisfactory acetabulum was obtained in 44 hips and the mean age of congruity was 33 months; the acetabulum was unsatisfactory in 30 hips, with a mean age of congruity of 48 months. Four years is the critical age, for if congruity is obtained later, the risk of producing a moderate or severely dysplastic acetabulum is more than doubled. If congruity is obtained under four years of age, growth of the acetabulum with continue in most patients up to 8 and in some to 11 YEARS OF age; the resulting acetabulum was normal or mildly dysplastic. Fifty-nine per cent of the satisfactory hips at follow-up had a normal anteversion angle, and 6 per cent a normal neck/shaft angle. Correction of these angles by themselves, seems not to be a major importance for promoting acetabular growth.", "PMID": 954330}
{"id": "pubmed23n0032_1084", "title": "Accidental iron poisoning in childhood. Six cases including one fatality.", "content": "Between 1962 and 1973, six children were admitted to the Massachusetts General Hospital because of accidental iron poisoning. Intoxication was life-threatening in two children whose serum iron concentrations exceeded their iron binding capacities. One of these patients died despite intensive supportive care and desferoxamine therapy. Although iron poisoning appears to be relatively uncommon, it can produce life-threatening and fatal intoxication in children.", "contents": "Accidental iron poisoning in childhood. Six cases including one fatality. Between 1962 and 1973, six children were admitted to the Massachusetts General Hospital because of accidental iron poisoning. Intoxication was life-threatening in two children whose serum iron concentrations exceeded their iron binding capacities. One of these patients died despite intensive supportive care and desferoxamine therapy. Although iron poisoning appears to be relatively uncommon, it can produce life-threatening and fatal intoxication in children.", "PMID": 954339}
{"id": "pubmed23n0032_1085", "title": "Congenital hyperammonemic syndromes.", "content": "Urea cycle disorders and other hyperammonemic syndromes should be considered in the differential diagnosis in newborns with a history of severe vomiting, lethargy, and seizures, and in infants with feeding problems, episodic vomiting, and altered consciousness. Although the acute neonatal forms of urea cycle disorders are almost always lethal, several of the subacute forms of hyperammonemic syndromes respond favorably to early treatment.", "contents": "Congenital hyperammonemic syndromes. Urea cycle disorders and other hyperammonemic syndromes should be considered in the differential diagnosis in newborns with a history of severe vomiting, lethargy, and seizures, and in infants with feeding problems, episodic vomiting, and altered consciousness. Although the acute neonatal forms of urea cycle disorders are almost always lethal, several of the subacute forms of hyperammonemic syndromes respond favorably to early treatment.", "PMID": 954343}
{"id": "pubmed23n0032_1086", "title": "Digoxin bioavailability: formulations and rates of infusions.", "content": "The bioavailability of digoxin (lanoxin) tablets, oral aqueous solution of digoxin, and capsules containing a solution of digoxin was compared with digoxin given intravenously over 1 and 3 hr. The mean peak serum concentration of digoxin after the 1-hr intravenous infusion was 5 ng/ml, after the 3-hr infusion, 3.5 ng/ml, and after the oral solution, 2.0 ng/ml. There was an equivalent bioavailability of the oral solution and reference tablets of digoxin. The digoxin in capsules tended to be better absorbed than the reference tablets. There was 21% more digoxin excreted over 6 days after the 3 hr iv infusion than after the 1 hr iv infusion. This indicates that the calculated bioavailability of an orally administered dose of digoxin may vary with the rapidity of injection of the intravenous standard. It is estimated that an oral tablet of digoxin of 0.5 mg has about the same bioavailability as 0.35 of digoxin given by slow intravenous infusion (or 0.4 mg if calculated against a rapid intravenous injection).", "contents": "Digoxin bioavailability: formulations and rates of infusions. The bioavailability of digoxin (lanoxin) tablets, oral aqueous solution of digoxin, and capsules containing a solution of digoxin was compared with digoxin given intravenously over 1 and 3 hr. The mean peak serum concentration of digoxin after the 1-hr intravenous infusion was 5 ng/ml, after the 3-hr infusion, 3.5 ng/ml, and after the oral solution, 2.0 ng/ml. There was an equivalent bioavailability of the oral solution and reference tablets of digoxin. The digoxin in capsules tended to be better absorbed than the reference tablets. There was 21% more digoxin excreted over 6 days after the 3 hr iv infusion than after the 1 hr iv infusion. This indicates that the calculated bioavailability of an orally administered dose of digoxin may vary with the rapidity of injection of the intravenous standard. It is estimated that an oral tablet of digoxin of 0.5 mg has about the same bioavailability as 0.35 of digoxin given by slow intravenous infusion (or 0.4 mg if calculated against a rapid intravenous injection).", "PMID": 954346}
{"id": "pubmed23n0032_1087", "title": "Absolute quinidine bioavailability.", "content": "The absolute bioavailability of quinidine was studied in 11 hospitalized patients. A 400-mg dose of quinidine gluconate was administered to each patient by intravenous infusion and as an oral solution. Drug treatments were separated by a 72-hr period. In 8 patients, peak plasma quinidine concentrations were reached in 65 min after the oral dose; in the remaining 3 subjects, peak concentrations were reached later. From the ratio of the total area under the plasma concentration-time curves (AUCoral/AUCir), the absolute bioavailability of quinidine ranged from 44% to 89% (mean, 72). In 8 patients, the ratio of the total amount of quinidine excreted in the urine in 48 hr (AUinfinity oral/AUinfinity ir) indicated that the extent of quinidine bioavailability varied form 47% to 96% (mean, 73). The predicted bioavailability of quindine due to first-pass effects was 76+/-11%. It is concluded that absorption after the oral solution was rapid and that the reduction of quinidine bioavailability was due to first-pass hepatic drug removal.", "contents": "Absolute quinidine bioavailability. The absolute bioavailability of quinidine was studied in 11 hospitalized patients. A 400-mg dose of quinidine gluconate was administered to each patient by intravenous infusion and as an oral solution. Drug treatments were separated by a 72-hr period. In 8 patients, peak plasma quinidine concentrations were reached in 65 min after the oral dose; in the remaining 3 subjects, peak concentrations were reached later. From the ratio of the total area under the plasma concentration-time curves (AUCoral/AUCir), the absolute bioavailability of quinidine ranged from 44% to 89% (mean, 72). In 8 patients, the ratio of the total amount of quinidine excreted in the urine in 48 hr (AUinfinity oral/AUinfinity ir) indicated that the extent of quinidine bioavailability varied form 47% to 96% (mean, 73). The predicted bioavailability of quindine due to first-pass effects was 76+/-11%. It is concluded that absorption after the oral solution was rapid and that the reduction of quinidine bioavailability was due to first-pass hepatic drug removal.", "PMID": 954347}
{"id": "pubmed23n0032_1088", "title": "Transient cardiac arrhythmias after single daily maintenance doses of digoxin.", "content": "Ten digitalized patients were monitored for detection of cardiac arrhythmias 0 to 8 hr after daily maintenance dose of digoxin in the fasting state. Transient cardiac arrhythmias attributable to digoxin were seen at 2 to 5 hr in 5 subjects. Initial serum concentrations of digoxin were within standard clinical limits in all subjects, but, higher steady-state levels were present in patients with arrhythmias (1.2 to 1.7 ng/ml) than in the others (0.7 to 1.2 ng/ml). The postadministrative serum peak concentrations were also higher in the patients with arrhythmias.", "contents": "Transient cardiac arrhythmias after single daily maintenance doses of digoxin. Ten digitalized patients were monitored for detection of cardiac arrhythmias 0 to 8 hr after daily maintenance dose of digoxin in the fasting state. Transient cardiac arrhythmias attributable to digoxin were seen at 2 to 5 hr in 5 subjects. Initial serum concentrations of digoxin were within standard clinical limits in all subjects, but, higher steady-state levels were present in patients with arrhythmias (1.2 to 1.7 ng/ml) than in the others (0.7 to 1.2 ng/ml). The postadministrative serum peak concentrations were also higher in the patients with arrhythmias.", "PMID": 954348}
{"id": "pubmed23n0032_1089", "title": "Pharmacokinetics of naproxen overdoses.", "content": "In earlier safety studies, naproxen, 600 mg three times daily, was administered to healthy subject without significant adverse effects. Another study demonstrated that single doses of 500 to 900 mg resulted in accelerated renal clearance and a nonlinear naproxen plasma level response after the higher doses. Our report describes the pharmacokinetics of naproxen when administered in single doses of 1, 2, 3, or 4 gm (up to eight times the clinically effective dose in rheumatoid arthritis) to healthy subjects. An increase in urinary excretion rate and continuation of the previously documented nonlinear plasma level response were observed. There were no signs that capacity to conjugate or to excrete the drug was exceeded. There were no adverse effects.", "contents": "Pharmacokinetics of naproxen overdoses. In earlier safety studies, naproxen, 600 mg three times daily, was administered to healthy subject without significant adverse effects. Another study demonstrated that single doses of 500 to 900 mg resulted in accelerated renal clearance and a nonlinear naproxen plasma level response after the higher doses. Our report describes the pharmacokinetics of naproxen when administered in single doses of 1, 2, 3, or 4 gm (up to eight times the clinically effective dose in rheumatoid arthritis) to healthy subjects. An increase in urinary excretion rate and continuation of the previously documented nonlinear plasma level response were observed. There were no signs that capacity to conjugate or to excrete the drug was exceeded. There were no adverse effects.", "PMID": 954349}
{"id": "pubmed23n0032_1090", "title": "Relationship between the pharmacokinetics and pharmacodynamics of procainamide.", "content": "The kinetics of a measure of pharmcologic effect (prolongation of the QT interval) of procainamide, as well as the kinetics of the plasma concentration, urine excretion, and saliva concentration of the drug were investigated in 14 trials in 4 subjects. A single 500-mg dose was given by rapid intravenous infusion, and frequent subsequent determinations of the above variables were made. A 2-compartment pharmacokinetic model with a third compartment for the saliva was used to fit the plasma, urine, and saliva data simultaneously. Analysis of the data reveals that the kinetics of the drug concentrations in saliva and of the pharmacologic effect are indistinguishable. They both must be considered to be different from those of the drug concentrations in plasma. Thus, in normal individuals under the conditions of this study, saliva concentrations more precisely indicate the time-course of drug at a cardiac site of action, although they do not parallel plasma drug concentrations until 6 hr or more after a rapid intravenous infusion. The following average pharmacokinetic parameters for plasma were found: terminal half-life, 2.9 hr; total clearance, 828 ml/min; renal clearance, 334 ml/min; and steady-state volume of distribution, 180 L. Average distribution pseudoequilbrium half-time (t1/2 alpha) was 5.2 min from an initial volume of distribution of 36.6 L.", "contents": "Relationship between the pharmacokinetics and pharmacodynamics of procainamide. The kinetics of a measure of pharmcologic effect (prolongation of the QT interval) of procainamide, as well as the kinetics of the plasma concentration, urine excretion, and saliva concentration of the drug were investigated in 14 trials in 4 subjects. A single 500-mg dose was given by rapid intravenous infusion, and frequent subsequent determinations of the above variables were made. A 2-compartment pharmacokinetic model with a third compartment for the saliva was used to fit the plasma, urine, and saliva data simultaneously. Analysis of the data reveals that the kinetics of the drug concentrations in saliva and of the pharmacologic effect are indistinguishable. They both must be considered to be different from those of the drug concentrations in plasma. Thus, in normal individuals under the conditions of this study, saliva concentrations more precisely indicate the time-course of drug at a cardiac site of action, although they do not parallel plasma drug concentrations until 6 hr or more after a rapid intravenous infusion. The following average pharmacokinetic parameters for plasma were found: terminal half-life, 2.9 hr; total clearance, 828 ml/min; renal clearance, 334 ml/min; and steady-state volume of distribution, 180 L. Average distribution pseudoequilbrium half-time (t1/2 alpha) was 5.2 min from an initial volume of distribution of 36.6 L.", "PMID": 954350}
{"id": "pubmed23n0032_1091", "title": "Influence of viral hepatitis on the disposition of two compounds with high hepatic clearance: lidocaine and indocyanine green.", "content": "The disposition of lidocaine and indocyanine green was studied in 6 individuals during and after recovery from an episode of acute viral hepatitis. Both compounds are highly cleared from the blood by the liver so that clearance of both should be sensitive to changes in hepatic blood flow. During the acute phase of illness, clearance of indocyanine green decreased without apparent change in volume of distribution, whereas clearance of lidocaine, decreased in 4, did not change in 1, and increased in 1 during the acute phase of hepatitis. Volume changes for lidocaine were also variable. We observed no significant correlation between any parameters of lidocaine disposition and any of several tests of liver function or any parameters of indocyanine green disposition. The absence of correlation between pharmacokinetic parameters of the disposition of lidocaine and indocyanine green indicates that the influence of hepatic disease on the hepatic processes that lead to the elimination of each compound is not predictable. No useful clinical correlates are now available by means of which to predict lidocaine disposition in patients with altered hepatic function.", "contents": "Influence of viral hepatitis on the disposition of two compounds with high hepatic clearance: lidocaine and indocyanine green. The disposition of lidocaine and indocyanine green was studied in 6 individuals during and after recovery from an episode of acute viral hepatitis. Both compounds are highly cleared from the blood by the liver so that clearance of both should be sensitive to changes in hepatic blood flow. During the acute phase of illness, clearance of indocyanine green decreased without apparent change in volume of distribution, whereas clearance of lidocaine, decreased in 4, did not change in 1, and increased in 1 during the acute phase of hepatitis. Volume changes for lidocaine were also variable. We observed no significant correlation between any parameters of lidocaine disposition and any of several tests of liver function or any parameters of indocyanine green disposition. The absence of correlation between pharmacokinetic parameters of the disposition of lidocaine and indocyanine green indicates that the influence of hepatic disease on the hepatic processes that lead to the elimination of each compound is not predictable. No useful clinical correlates are now available by means of which to predict lidocaine disposition in patients with altered hepatic function.", "PMID": 954351}
{"id": "pubmed23n0032_1092", "title": "Intrasubject variation of warfarin binding to protein in serum of patients with cardiovascular disease.", "content": "A study of 31 patients with cardiovascular disease who were taking warfarin regularly had shown pronounced intersubject differences in serum protein binding of warfarin and a highly significant correlation between the body clearance of warfarin and the free fraction of the drug in serum. Similar observations have been made in experimental animals and are consistent with predictions based on theoretical considerations. The purpose of this investigation was to determine the intrasubject variation in the free fraction of warfarin in serum. Samples of serum were obtained from 23 of the 31 patients previously studied. The time interval between the two studies was 3.4 to 5.7 mo. The daily dose of warfarin had been changed by 10.6% on the average. With two exceptions, there was no change in concurrent medications. The ratio of free fraction values of warfarin in serum, second/first study, was 0.948 +/- 0.297 (mean +/- S.D.), and there was a highly significant correlation (P less than 0.001) between the individual free fraction values in the first and second studies.", "contents": "Intrasubject variation of warfarin binding to protein in serum of patients with cardiovascular disease. A study of 31 patients with cardiovascular disease who were taking warfarin regularly had shown pronounced intersubject differences in serum protein binding of warfarin and a highly significant correlation between the body clearance of warfarin and the free fraction of the drug in serum. Similar observations have been made in experimental animals and are consistent with predictions based on theoretical considerations. The purpose of this investigation was to determine the intrasubject variation in the free fraction of warfarin in serum. Samples of serum were obtained from 23 of the 31 patients previously studied. The time interval between the two studies was 3.4 to 5.7 mo. The daily dose of warfarin had been changed by 10.6% on the average. With two exceptions, there was no change in concurrent medications. The ratio of free fraction values of warfarin in serum, second/first study, was 0.948 +/- 0.297 (mean +/- S.D.), and there was a highly significant correlation (P less than 0.001) between the individual free fraction values in the first and second studies.", "PMID": 954352}
{"id": "pubmed23n0032_1093", "title": "Naltrexone: disposition, metabolism, and effects after acute and chronic dosing.", "content": "The disposition of naltrexone during acute and chronic administration of 100-mg oral dose was studied in 4 subjects. Following an acute dose the mean (X) peak naltrexone plasma level was 43.6 +/- 29.9 ng/ml at 1 hr and for the major biotransformation product, beta-naltrexol, was 87.2 +/- 25.0 ng/ml at 2 hr. Twenty-four hours after the dose the X levels of naltrexone and beta-naltrexol declined to 2.1 +/- 0.47 and 17.6 +/- 5.0 ng/ml, respectively. Following chronic administration and X peak plasma levels of naltrexone and beta-naltrexol rose to 46.4 +/- 18.5 and 158.4 +/- 89.9 ng/ml at 1 hr, but by 24 hr both compounds declined to levels of the same order as in the acute state at 24 hr. Plasma levels of naltrexone and beta-naltrexol measured 24 hr after the daily doses of naltrexone throughout the study indicated that steady-state equilibrium was rapidly attained and that there was no accumulation of naltrexone and beta naltrexol in the plasma after chronic treatment on 100 mg oral doses. Biexponential kinetics were observed for naltrexone and beta-naltrexol in the first 24 hr. The half-life of naltrexone and beta-naltrexol decreased slightly from the acute to thechronic study from 10.3 +/- 3.3 to 9.7 +/- 1.1 hr and from 12.7 +/- 2.6 to 11.4 +/- 2.0 hr. The plasma levels of naltrexone declined slowly from 24 through 72 hr from 2.4 to 1.7 ng/ml, with an apparent half-life of 96 hr. The renal clearance data indicate that naltrexone is partially reabsorbed while beta naltrexol is actively secreted by the kidney. During acute and chronic naltrexone administration the mean fecal excretion was 2.1% and 3.6% while urinary excretion was 38% and 70% of the dose in a 24-hr period. Opiate antagonism to 25 mg heroin challenges was nearly complete through 48 hr after naltrexone. At 72 hr the objective responses reappeared to a greater extent than the subjective ones. Correlation coefficient (r) between naltrexone plasma levels and opiate antagonism was 0.91 and between individual half-life of naltrexone and opiate antagonism it was 0.99.", "contents": "Naltrexone: disposition, metabolism, and effects after acute and chronic dosing. The disposition of naltrexone during acute and chronic administration of 100-mg oral dose was studied in 4 subjects. Following an acute dose the mean (X) peak naltrexone plasma level was 43.6 +/- 29.9 ng/ml at 1 hr and for the major biotransformation product, beta-naltrexol, was 87.2 +/- 25.0 ng/ml at 2 hr. Twenty-four hours after the dose the X levels of naltrexone and beta-naltrexol declined to 2.1 +/- 0.47 and 17.6 +/- 5.0 ng/ml, respectively. Following chronic administration and X peak plasma levels of naltrexone and beta-naltrexol rose to 46.4 +/- 18.5 and 158.4 +/- 89.9 ng/ml at 1 hr, but by 24 hr both compounds declined to levels of the same order as in the acute state at 24 hr. Plasma levels of naltrexone and beta-naltrexol measured 24 hr after the daily doses of naltrexone throughout the study indicated that steady-state equilibrium was rapidly attained and that there was no accumulation of naltrexone and beta naltrexol in the plasma after chronic treatment on 100 mg oral doses. Biexponential kinetics were observed for naltrexone and beta-naltrexol in the first 24 hr. The half-life of naltrexone and beta-naltrexol decreased slightly from the acute to thechronic study from 10.3 +/- 3.3 to 9.7 +/- 1.1 hr and from 12.7 +/- 2.6 to 11.4 +/- 2.0 hr. The plasma levels of naltrexone declined slowly from 24 through 72 hr from 2.4 to 1.7 ng/ml, with an apparent half-life of 96 hr. The renal clearance data indicate that naltrexone is partially reabsorbed while beta naltrexol is actively secreted by the kidney. During acute and chronic naltrexone administration the mean fecal excretion was 2.1% and 3.6% while urinary excretion was 38% and 70% of the dose in a 24-hr period. Opiate antagonism to 25 mg heroin challenges was nearly complete through 48 hr after naltrexone. At 72 hr the objective responses reappeared to a greater extent than the subjective ones. Correlation coefficient (r) between naltrexone plasma levels and opiate antagonism was 0.91 and between individual half-life of naltrexone and opiate antagonism it was 0.99.", "PMID": 954353}
{"id": "pubmed23n0032_1094", "title": "The enantiomers of phenprocoumon: pharmacodynamic and pharmacokinetic studies.", "content": "The pharmacodynamics and pharmacokinetics of the optical enantiomers of phenprocoumon were studied in 5 normal subjects and compared to the racemic mixture. Each subject received a single oral dose of 0.6 mg/kg of racemic, S(-), and R(+) phenprocoumon. S(-) phenprocoumon was 1.6 to 2.6 times as a potent as R(+) phenprocoumon when the area under the effect/time curve was used to quantify the total anticoagulant effect per dose. Comparing the plasma concentrations that elicited the same anticoagulant effect, S(-) phenprocoumon was 1.5 to 2.5 times as potent as R(+) phenprocoumon. The anticoagulant activity of the racemic mixture was between that of the enantiomers. There was no distinct difference in the rate of elimination between the enantiomers. The apparent volume of distribution and the plasma clearance for S(-) phenprocoumon were less than those for R(+) phenprocoumon. When the binding of the enantiomers to human serum albumin was compared, S(-) phenprocoumon was more highly bound than R(+) phenprocoumon. The protein binding of racemic phenprocoumon was between that of the enantiomers. The results show that S(-) phenprocoumon is more potent anticoagulant than R(+) phenprocoumon and that the pharmacokinetic differences between the enantiomers are due mainly to differences in their distribution.", "contents": "The enantiomers of phenprocoumon: pharmacodynamic and pharmacokinetic studies. The pharmacodynamics and pharmacokinetics of the optical enantiomers of phenprocoumon were studied in 5 normal subjects and compared to the racemic mixture. Each subject received a single oral dose of 0.6 mg/kg of racemic, S(-), and R(+) phenprocoumon. S(-) phenprocoumon was 1.6 to 2.6 times as a potent as R(+) phenprocoumon when the area under the effect/time curve was used to quantify the total anticoagulant effect per dose. Comparing the plasma concentrations that elicited the same anticoagulant effect, S(-) phenprocoumon was 1.5 to 2.5 times as potent as R(+) phenprocoumon. The anticoagulant activity of the racemic mixture was between that of the enantiomers. There was no distinct difference in the rate of elimination between the enantiomers. The apparent volume of distribution and the plasma clearance for S(-) phenprocoumon were less than those for R(+) phenprocoumon. When the binding of the enantiomers to human serum albumin was compared, S(-) phenprocoumon was more highly bound than R(+) phenprocoumon. The protein binding of racemic phenprocoumon was between that of the enantiomers. The results show that S(-) phenprocoumon is more potent anticoagulant than R(+) phenprocoumon and that the pharmacokinetic differences between the enantiomers are due mainly to differences in their distribution.", "PMID": 954354}
{"id": "pubmed23n0032_1095", "title": "Paradoxical urinary excretion of D-glucaric acid in acute viral hepatitis.", "content": "The urinary excretion of D-glucaric acid and the plasma clearance of antipyrine were estimated during the acute phase of viral hepatitis and again during recovery. The plasma clearance of antipyrine was impaired during the acute stage of hepatitis, while the urinary excretion of D-glucaric acid was paradoxically high. Both parameters returned to normal during recovery. These findings suggest that the use of urinary D-glucaric acid excretion as an index of microsomal enzyme induction is unreliable when there is liver injury.", "contents": "Paradoxical urinary excretion of D-glucaric acid in acute viral hepatitis. The urinary excretion of D-glucaric acid and the plasma clearance of antipyrine were estimated during the acute phase of viral hepatitis and again during recovery. The plasma clearance of antipyrine was impaired during the acute stage of hepatitis, while the urinary excretion of D-glucaric acid was paradoxically high. Both parameters returned to normal during recovery. These findings suggest that the use of urinary D-glucaric acid excretion as an index of microsomal enzyme induction is unreliable when there is liver injury.", "PMID": 954355}
{"id": "pubmed23n0032_1096", "title": "Factors affecting antipyrine metabolism in West African villagers.", "content": "Saliva half-life of antipyrine was studied in 49 healthy Gambians between 20 and 60 yr of age of whom 27 were male (mean age, 44.5) and 22 female (mean age, 39.1). Body wieght, height, ponderal index, albumin, and hemoglobin were moderately reduced compared to accepted normal values. Antipyrine half-life was 13.6 +/- 0.58 (SEM) hr. Multiple regression analysis showed that sex, cola nut consumption, hemoglobin in women, and height in men were statiscally significant independent predictors of antipyrine half-life. Half-life was shorter in women, decreased with an increase in height in men, and was prolonged by cola nut consumption. Half-life in women increased with hemoglobin. These factors explained 36% of the variation and suggest that geographic differences in the environment could be important in drug metabolism in man.", "contents": "Factors affecting antipyrine metabolism in West African villagers. Saliva half-life of antipyrine was studied in 49 healthy Gambians between 20 and 60 yr of age of whom 27 were male (mean age, 44.5) and 22 female (mean age, 39.1). Body wieght, height, ponderal index, albumin, and hemoglobin were moderately reduced compared to accepted normal values. Antipyrine half-life was 13.6 +/- 0.58 (SEM) hr. Multiple regression analysis showed that sex, cola nut consumption, hemoglobin in women, and height in men were statiscally significant independent predictors of antipyrine half-life. Half-life was shorter in women, decreased with an increase in height in men, and was prolonged by cola nut consumption. Half-life in women increased with hemoglobin. These factors explained 36% of the variation and suggest that geographic differences in the environment could be important in drug metabolism in man.", "PMID": 954356}
{"id": "pubmed23n0032_1097", "title": "A method for measurement of angiotensin II in tissues and its application to rat kidney.", "content": "1. A method for extraction, partial purification and radioimmunoassay of angiotensin II in tissues and application of the method to the kidneys of sodium-deficient rats are described. 2. Angiotensin in acid-ethanol extracts of kidney were adsorbed on to a cation-exchange resin, eluted and further purified with an immobilized angiotensin II antiserum, before radioimmunoassay. 3. Thin-layer chromatography was used, in some experiments, to separate fmol amounts of angiotensin II from its immunoreactive peptide fragments before radioimmunoassay. 4. Angiotensin II-immunoreactive material isolated from rat kidney resembled angiotensin II in many of its physicochemical properties and chromatographic mobility. 5. The concentration of immunoreactive material in kidney greatly exceeded that which could be accounted for by trapped blood and suggests that the peptide may have a local role in the organ.", "contents": "A method for measurement of angiotensin II in tissues and its application to rat kidney. 1. A method for extraction, partial purification and radioimmunoassay of angiotensin II in tissues and application of the method to the kidneys of sodium-deficient rats are described. 2. Angiotensin in acid-ethanol extracts of kidney were adsorbed on to a cation-exchange resin, eluted and further purified with an immobilized angiotensin II antiserum, before radioimmunoassay. 3. Thin-layer chromatography was used, in some experiments, to separate fmol amounts of angiotensin II from its immunoreactive peptide fragments before radioimmunoassay. 4. Angiotensin II-immunoreactive material isolated from rat kidney resembled angiotensin II in many of its physicochemical properties and chromatographic mobility. 5. The concentration of immunoreactive material in kidney greatly exceeded that which could be accounted for by trapped blood and suggests that the peptide may have a local role in the organ.", "PMID": 954357}
{"id": "pubmed23n0032_1098", "title": "The absorption of sugars from the human buccal cavity.", "content": "1. Certain sugars were transported across the buccal mucosa by a carrier-mediated mechanism. 2. The metabolic loss of sugars from the mouth in a 5 min test period was negligible. 3. The buccal mucosal transport process was stereospecific for D-glucose and L-arabinose. 4. The absorption of D-glucose, galactose and 3-O-methyl-D-glucose was at least partly dependent on the presence of sodium ions in the luminal fluid. 5. The transport of D-glucose, was inhibited by galactose and 3-O-methyl-D-glucose, suggesting at least one common carrier system.", "contents": "The absorption of sugars from the human buccal cavity. 1. Certain sugars were transported across the buccal mucosa by a carrier-mediated mechanism. 2. The metabolic loss of sugars from the mouth in a 5 min test period was negligible. 3. The buccal mucosal transport process was stereospecific for D-glucose and L-arabinose. 4. The absorption of D-glucose, galactose and 3-O-methyl-D-glucose was at least partly dependent on the presence of sodium ions in the luminal fluid. 5. The transport of D-glucose, was inhibited by galactose and 3-O-methyl-D-glucose, suggesting at least one common carrier system.", "PMID": 954358}
{"id": "pubmed23n0032_1099", "title": "Density-dependence of maximal expiratory flow rates before and after bronchodilators in patients with obstructive airways disease.", "content": "1.Gas-density-dependence of maximal expiratory flow rats (V max), defined as the ratio of V max. while breathing helium/oxygen (80:20) to V max. while breathing air at the same lung volume, was examined in relation to other measurements of airways obstruction in patients with obstructive airways disease before and after administration of bronchodilators. 2. Seventeen patients showed a 45% or greater increase in specific conductance (sG aw) after bronchodilator therapy (group A) and thirteen patients demonstrated a lesser response (group B). 3. Before the administration of bronchodilators, the degree of obstruction in two groups was not different as measured by lung volumes, sG aw, forced expiratory volume in 1 s, and flow rates high in the vital capacity; yet the maximal mid-expiratory flow rate and the degree of density-dependence were significantly lower in group B. 4. After bronchodilators, both groups of patients showed significant improvements in sG aw, flow rates and lung volumes. However, group A patients showed a signifcant increase in density-dependence whereas group B patients did not. 5. Increased density-dependence after bronchodilators in the group A patients was associated with an increase in the computed resistance of the upstream segment with air and a decrease in resistance with helium/oxygen. These changes could be explained by a more mouthward of equal pressure points, and therefore a further increase in the relative contribution of the larger density-dependent airways to limitation of flow. 6. The fact that density-dependence was not altered after bronchodilators in the group B patients suggests that the site of limitation of flow did not change appreciably. The shift in the pressure-flow curve for the upstream airways was such that the computed resistance of these airways fell. Thus it appears that the airways comprising the upstream segment were dilated.", "contents": "Density-dependence of maximal expiratory flow rates before and after bronchodilators in patients with obstructive airways disease. 1.Gas-density-dependence of maximal expiratory flow rats (V max), defined as the ratio of V max. while breathing helium/oxygen (80:20) to V max. while breathing air at the same lung volume, was examined in relation to other measurements of airways obstruction in patients with obstructive airways disease before and after administration of bronchodilators. 2. Seventeen patients showed a 45% or greater increase in specific conductance (sG aw) after bronchodilator therapy (group A) and thirteen patients demonstrated a lesser response (group B). 3. Before the administration of bronchodilators, the degree of obstruction in two groups was not different as measured by lung volumes, sG aw, forced expiratory volume in 1 s, and flow rates high in the vital capacity; yet the maximal mid-expiratory flow rate and the degree of density-dependence were significantly lower in group B. 4. After bronchodilators, both groups of patients showed significant improvements in sG aw, flow rates and lung volumes. However, group A patients showed a signifcant increase in density-dependence whereas group B patients did not. 5. Increased density-dependence after bronchodilators in the group A patients was associated with an increase in the computed resistance of the upstream segment with air and a decrease in resistance with helium/oxygen. These changes could be explained by a more mouthward of equal pressure points, and therefore a further increase in the relative contribution of the larger density-dependent airways to limitation of flow. 6. The fact that density-dependence was not altered after bronchodilators in the group B patients suggests that the site of limitation of flow did not change appreciably. The shift in the pressure-flow curve for the upstream airways was such that the computed resistance of these airways fell. Thus it appears that the airways comprising the upstream segment were dilated.", "PMID": 954359}
{"id": "pubmed23n0032_1100", "title": "Measurement of liver blood flow by means of a single injection of bromosulphthalein, without hepatic venous catheterization.", "content": "1. A method is described for the measurement of liver blood flow without hepatic venous catheterization by the injection of the a single dose of bromosulphthalein and the analysis of the graph of the plasma disappearance of the indicator. 2. The rationale of the method was tested in three systems which covered a wide range of flow rates. The first system was a physical model with a haemodialyser in which the calculated flow could be compared with the flow measured directly. The second was in anesthetized dogs, in which the calculated flow was compared with that measured by continuous infusion and hepatic venous catheterization, based on the Fick principle. The third system was in unanaesthetized turkeys, in which metabolism of bromosulphthalein does not occur. The calculated flow was compared with the flow measured simultaneously by the uptake of collodial particles by the Kupffer cells. 3. In all three systems the correspondence between the two methods of measuring liver blood flow was good, the differences between them being not statistically significant. 4. The method has been applied to measurements of liver blood flow in man and animals and gives results comparable with those reported by other workers.", "contents": "Measurement of liver blood flow by means of a single injection of bromosulphthalein, without hepatic venous catheterization. 1. A method is described for the measurement of liver blood flow without hepatic venous catheterization by the injection of the a single dose of bromosulphthalein and the analysis of the graph of the plasma disappearance of the indicator. 2. The rationale of the method was tested in three systems which covered a wide range of flow rates. The first system was a physical model with a haemodialyser in which the calculated flow could be compared with the flow measured directly. The second was in anesthetized dogs, in which the calculated flow was compared with that measured by continuous infusion and hepatic venous catheterization, based on the Fick principle. The third system was in unanaesthetized turkeys, in which metabolism of bromosulphthalein does not occur. The calculated flow was compared with the flow measured simultaneously by the uptake of collodial particles by the Kupffer cells. 3. In all three systems the correspondence between the two methods of measuring liver blood flow was good, the differences between them being not statistically significant. 4. The method has been applied to measurements of liver blood flow in man and animals and gives results comparable with those reported by other workers.", "PMID": 954360}
{"id": "pubmed23n0032_1101", "title": "Comparison of renal blood flow and transit times measured by means of 99mTc-labelled erythrocytes and Indocyanine Green in humans with normal and diseased kidneys.", "content": "1. In nineteen patients with normal or diseased kidneys, renal blood flow, transit times and vascular volume were determined by means of an indicator-dilution method. Two different indicators, plasma-bound Indocyanine Green (IG) and 99mTc-labelled erythrocytes, were used simultaneously. 2. Comparison of the results indicates that IG slightly overestimates renal blood flow, appearance time, mean transit time and vascular volume, as the erythrocyte/IG ratios averaged 0.972, 0.903, 0.93, and 0.921 respectively. Overestimation of the mean transit time was less apparent when it was prolonged. In patients with reduced renal function, the average blood flow values obtained with the two indicators were in good agreement. 3. It is unlikely that axial streaming of erythrocytes accounts for their shorter mean transit time, because the individual erythrocyte/IG mean transit time ratios were independent of the rate of blood flow and the peripheral packed cell volume. 4. Since the erythrocyte/IG mean transit time ratios correlated significantly with the erythrocyte/IG ratios for appearance time and renal blood flow, the common mechanism leading to a depression of all erythrocyte/IG ratios is presumably extravascular circulation and delayed recovery of a small fraction of IG.", "contents": "Comparison of renal blood flow and transit times measured by means of 99mTc-labelled erythrocytes and Indocyanine Green in humans with normal and diseased kidneys. 1. In nineteen patients with normal or diseased kidneys, renal blood flow, transit times and vascular volume were determined by means of an indicator-dilution method. Two different indicators, plasma-bound Indocyanine Green (IG) and 99mTc-labelled erythrocytes, were used simultaneously. 2. Comparison of the results indicates that IG slightly overestimates renal blood flow, appearance time, mean transit time and vascular volume, as the erythrocyte/IG ratios averaged 0.972, 0.903, 0.93, and 0.921 respectively. Overestimation of the mean transit time was less apparent when it was prolonged. In patients with reduced renal function, the average blood flow values obtained with the two indicators were in good agreement. 3. It is unlikely that axial streaming of erythrocytes accounts for their shorter mean transit time, because the individual erythrocyte/IG mean transit time ratios were independent of the rate of blood flow and the peripheral packed cell volume. 4. Since the erythrocyte/IG mean transit time ratios correlated significantly with the erythrocyte/IG ratios for appearance time and renal blood flow, the common mechanism leading to a depression of all erythrocyte/IG ratios is presumably extravascular circulation and delayed recovery of a small fraction of IG.", "PMID": 954361}
{"id": "pubmed23n0032_1102", "title": "The effect of cellulose phosphate on plasma and urinary magnesium at different levels of parathyroid function in man.", "content": "1. Previously published data obtained by magnesium infusion in man were found to conform to a Tm/glomerular filtration rate (GFR) model on the assumption of 80% diffusibility of plasma magnesium. The lower limit of Tm,Mg/GFR was 625 mumol/l. 2. Previously published data concerning the effect of cellulose phosphate on magnesium metabolism in normal subjects, patients with latent hypoparathyroidism and patients with primary hyperparathyroidism were found to conform to the same model, with the same limit for Tm,Mg/GFR for all three levels of parathyroid function. 3. The threshold for magnesium excretion is sharper with less 'splay' than for phosphate, but as for phosophate it is close to the normal blood concentration. 4. Because of the geometrical relationship between different methods of presentation of data, at a constant value for Tm,Mg/GFR changes in magnesium load or in GFR automatically produce changes in fractional magnesium clearance. This is the explanation for the increase in fractional magnesium clearance which occurs which with diminishing renal function. 5. Renal conservation of magnesium is a passive consequence of the fall in plasma magnesium. There was no evidence of augmented tubular reabsorption of magnesium in response to magnesium deprivation in any of the three groups of subjects. 6. The tubular reabsorption of magnesium was not altered detectably by a moderate deficiency or excess of parathyroid hormore. Changes in parathyroid hormone secretion are probably not concerned in normal magnesium homeostasis.", "contents": "The effect of cellulose phosphate on plasma and urinary magnesium at different levels of parathyroid function in man. 1. Previously published data obtained by magnesium infusion in man were found to conform to a Tm/glomerular filtration rate (GFR) model on the assumption of 80% diffusibility of plasma magnesium. The lower limit of Tm,Mg/GFR was 625 mumol/l. 2. Previously published data concerning the effect of cellulose phosphate on magnesium metabolism in normal subjects, patients with latent hypoparathyroidism and patients with primary hyperparathyroidism were found to conform to the same model, with the same limit for Tm,Mg/GFR for all three levels of parathyroid function. 3. The threshold for magnesium excretion is sharper with less 'splay' than for phosphate, but as for phosophate it is close to the normal blood concentration. 4. Because of the geometrical relationship between different methods of presentation of data, at a constant value for Tm,Mg/GFR changes in magnesium load or in GFR automatically produce changes in fractional magnesium clearance. This is the explanation for the increase in fractional magnesium clearance which occurs which with diminishing renal function. 5. Renal conservation of magnesium is a passive consequence of the fall in plasma magnesium. There was no evidence of augmented tubular reabsorption of magnesium in response to magnesium deprivation in any of the three groups of subjects. 6. The tubular reabsorption of magnesium was not altered detectably by a moderate deficiency or excess of parathyroid hormore. Changes in parathyroid hormone secretion are probably not concerned in normal magnesium homeostasis.", "PMID": 954362}
{"id": "pubmed23n0032_1103", "title": "Kinetics of hepatic uptake of unconjugated bilirubin.", "content": "1. The uptake of bilirubin was studied in the perfused rat liver by a multiple-indicator dilution technique employing the three-compartment model of Goresky. 2. The kinetics of hepatic bilirubin uptake could be described by the Michaelis-Menten equation. 3. The maximal uptake velocity (V max.) and the apparent half-saturation constant (Km) were 4-4 +/- 0-5 nmol s-1 g-1 of liver and 58 +/- 16 nmol/g of liver respectively, indicating that the hepatic uptake system for bilirubin under normal conditions is operating far below saturation. 4. Sodium taurocholate did not compete with bilirubin for hepatic uptake. 5. These findings are consistent with the concept that carrier-mediated transport is responsible for hepatocellular uptake of bilirubin and that bilirubin and bile acids enter the hepatocyte via separate pathways.", "contents": "Kinetics of hepatic uptake of unconjugated bilirubin. 1. The uptake of bilirubin was studied in the perfused rat liver by a multiple-indicator dilution technique employing the three-compartment model of Goresky. 2. The kinetics of hepatic bilirubin uptake could be described by the Michaelis-Menten equation. 3. The maximal uptake velocity (V max.) and the apparent half-saturation constant (Km) were 4-4 +/- 0-5 nmol s-1 g-1 of liver and 58 +/- 16 nmol/g of liver respectively, indicating that the hepatic uptake system for bilirubin under normal conditions is operating far below saturation. 4. Sodium taurocholate did not compete with bilirubin for hepatic uptake. 5. These findings are consistent with the concept that carrier-mediated transport is responsible for hepatocellular uptake of bilirubin and that bilirubin and bile acids enter the hepatocyte via separate pathways.", "PMID": 954363}
{"id": "pubmed23n0032_1104", "title": "Plasma enteroglucagon and plasma volume change after gastric surgery.", "content": "1. The changes in plasma enteroglucagon concentration and plasma volume were measured after oral glucose in sixteen subjects before and forty-eight subjects after operative treatment for benign duodenal ulceration. Truncal vagotomy with a drainage procedure had been performed in sixteen of the postoperative subjects studied, selective vagotomy with a drainage procedure in seventeen, and partial gastrectomy in fifteen. 2. Patients in whom the glucose produced dumping symptoms had significantly greater enteroglucagon concentrations than those who were symptom-free, which confirms the results of a preliminary study. There was a highly significant correlation between the rate of rise of plasma volume. 3. There was no difference in the rate of rise or in peak plasma enteroglucagon and the rate of fall of plasma volume. 3. There was no difference in the rate of rise or in peak plasma enteroglucagon concentration between the groups of patients who had different operative procedures. This suggests that enteroglucagon release is not under vagal influence. 4. There was no apparent relation between the plasma enteroglucagon concentration and the time-interval between the operation and the time of study, which implies that the exaggerated release of enteroglucagon after operation occurs in response to the altered gastric physiology and that there is minimal adaptive change.", "contents": "Plasma enteroglucagon and plasma volume change after gastric surgery. 1. The changes in plasma enteroglucagon concentration and plasma volume were measured after oral glucose in sixteen subjects before and forty-eight subjects after operative treatment for benign duodenal ulceration. Truncal vagotomy with a drainage procedure had been performed in sixteen of the postoperative subjects studied, selective vagotomy with a drainage procedure in seventeen, and partial gastrectomy in fifteen. 2. Patients in whom the glucose produced dumping symptoms had significantly greater enteroglucagon concentrations than those who were symptom-free, which confirms the results of a preliminary study. There was a highly significant correlation between the rate of rise of plasma volume. 3. There was no difference in the rate of rise or in peak plasma enteroglucagon and the rate of fall of plasma volume. 3. There was no difference in the rate of rise or in peak plasma enteroglucagon concentration between the groups of patients who had different operative procedures. This suggests that enteroglucagon release is not under vagal influence. 4. There was no apparent relation between the plasma enteroglucagon concentration and the time-interval between the operation and the time of study, which implies that the exaggerated release of enteroglucagon after operation occurs in response to the altered gastric physiology and that there is minimal adaptive change.", "PMID": 954364}
{"id": "pubmed23n0032_1105", "title": "The kinetics of efflux of 5,5-dimethyl-2,4-oxazolidinedione (DMO) from the myocardium.", "content": "1. The efflux of 14C-labelled 5,5-dimethyl-2,4-oxazolidinedione (DMO) from the myocardium of the rabbit has been studied. The perfusate pH was 7-38. 2. The effluent curve is complex and appears to be the resultant of movement between at least four compartments. 3. The first two probably represent intravascular and extracellular compartments; the last two have smaller rate constants and may represent intracellular spaces. Intracellular pH (pHi) calculated from the effluent curve was 7-23 +/- 0-05. pHi estimated from the steady-state distribution volume of DMO was 7-28 +/- 0-02. The existence of two intracellular compartments suggests that DMO is not homogeneously distrubted in the myocardium. It is suggested that the apparent greater buffering capacity of cardiac than skeletal muscle can be explained by the greater number and volume of mitochondria in the myocardium, compartmentalization of DMO and assumptions inherent in the DMO method for the measurement of pHi.", "contents": "The kinetics of efflux of 5,5-dimethyl-2,4-oxazolidinedione (DMO) from the myocardium. 1. The efflux of 14C-labelled 5,5-dimethyl-2,4-oxazolidinedione (DMO) from the myocardium of the rabbit has been studied. The perfusate pH was 7-38. 2. The effluent curve is complex and appears to be the resultant of movement between at least four compartments. 3. The first two probably represent intravascular and extracellular compartments; the last two have smaller rate constants and may represent intracellular spaces. Intracellular pH (pHi) calculated from the effluent curve was 7-23 +/- 0-05. pHi estimated from the steady-state distribution volume of DMO was 7-28 +/- 0-02. The existence of two intracellular compartments suggests that DMO is not homogeneously distrubted in the myocardium. It is suggested that the apparent greater buffering capacity of cardiac than skeletal muscle can be explained by the greater number and volume of mitochondria in the myocardium, compartmentalization of DMO and assumptions inherent in the DMO method for the measurement of pHi.", "PMID": 954365}
{"id": "pubmed23n0032_1106", "title": "Bone-resorbing activity in the sera of patients with rheumatoid arthritis.", "content": "1. Foetal rat hemi-calvaria were incubated in organ culture with sera from patients with active rheumatoid arthritis. 2. Increased 45Ca resorption was produced by sera from patients who were hypercalcaemic. 3. This bone-resorbing effect could be inhibited by calcitonin.", "contents": "Bone-resorbing activity in the sera of patients with rheumatoid arthritis. 1. Foetal rat hemi-calvaria were incubated in organ culture with sera from patients with active rheumatoid arthritis. 2. Increased 45Ca resorption was produced by sera from patients who were hypercalcaemic. 3. This bone-resorbing effect could be inhibited by calcitonin.", "PMID": 954367}
{"id": "pubmed23n0032_1107", "title": "Relationship of epicardial ST segment elevation to the plasma free fatty acid/albumin ratio during coronary occlusion in dogs.", "content": "1. Intravenous infusions of lipid-free albumin were used to lower the arterial non-esterified fatty acids (NEFA)/albumin ratio during coronary occlusion in dogs. 2. Reduction of the NEFA/albumin ratio was accompanied by proportionate reductions in myocardical uptake of NEFA and the degree of ST segment elevation in epicardial electrocardiographic recordings from the ischaemic zone. Aortic pressure and heart rate were unchanged. 3. Infusions of control albumin preparations had no effect on the NEFA/albumin ratio or ST segment elevation. 4. These observations support the proposition that the disturbance of cellular function during myocardial ischaemia is influenced by the degree of saturation of albumin-binding sites with NEFA and by their availability.", "contents": "Relationship of epicardial ST segment elevation to the plasma free fatty acid/albumin ratio during coronary occlusion in dogs. 1. Intravenous infusions of lipid-free albumin were used to lower the arterial non-esterified fatty acids (NEFA)/albumin ratio during coronary occlusion in dogs. 2. Reduction of the NEFA/albumin ratio was accompanied by proportionate reductions in myocardical uptake of NEFA and the degree of ST segment elevation in epicardial electrocardiographic recordings from the ischaemic zone. Aortic pressure and heart rate were unchanged. 3. Infusions of control albumin preparations had no effect on the NEFA/albumin ratio or ST segment elevation. 4. These observations support the proposition that the disturbance of cellular function during myocardial ischaemia is influenced by the degree of saturation of albumin-binding sites with NEFA and by their availability.", "PMID": 954368}
{"id": "pubmed23n0032_1108", "title": "How effective is safety packaging?", "content": "Of 96 ingestions involving safety packaging, 82% involved misuse. The package in some way was unacceptable to the consumer--it was too difficult to open or too difficult to close. Nonacceptance by the elderly was not a significant factor. In only 18% of the safety packaged ingestions, did the child upen the package. The child was more likely to be able to open the screw-cap and the strip-pack. The pop-off and press-lift were not opened by any child but were types misused only by parents. The older child with a record of prior poisoning was most likely to open a safety package. These children would appear to represent a hard core of risk subjects refractory even to safety packaging. Safety packaging has had a dramatic effect on the morbidity and mortality of accidental poisoning. There are two remaining problems that require further study: 1. The analysis of technical factors impeding consumer acceptance and child proofing. The ideal package is so easily handled by the adult that misuse does not occur, but is too difficult for the child to open. 2. The personality characteristics of the safety-package-resistant child. Safety packaging, as implemented by the Comsumer Product Safety Commission, has had remarkable success. Education did not reduce accidental poisoning; safety packaging does. Pediatricians, pharmacists, and toxicologists must work with industry and the Consumer Product Safety Commission to complete the goal of elimination of accidental poisoning.", "contents": "How effective is safety packaging? Of 96 ingestions involving safety packaging, 82% involved misuse. The package in some way was unacceptable to the consumer--it was too difficult to open or too difficult to close. Nonacceptance by the elderly was not a significant factor. In only 18% of the safety packaged ingestions, did the child upen the package. The child was more likely to be able to open the screw-cap and the strip-pack. The pop-off and press-lift were not opened by any child but were types misused only by parents. The older child with a record of prior poisoning was most likely to open a safety package. These children would appear to represent a hard core of risk subjects refractory even to safety packaging. Safety packaging has had a dramatic effect on the morbidity and mortality of accidental poisoning. There are two remaining problems that require further study: 1. The analysis of technical factors impeding consumer acceptance and child proofing. The ideal package is so easily handled by the adult that misuse does not occur, but is too difficult for the child to open. 2. The personality characteristics of the safety-package-resistant child. Safety packaging, as implemented by the Comsumer Product Safety Commission, has had remarkable success. Education did not reduce accidental poisoning; safety packaging does. Pediatricians, pharmacists, and toxicologists must work with industry and the Consumer Product Safety Commission to complete the goal of elimination of accidental poisoning.", "PMID": 954370}
{"id": "pubmed23n0032_1109", "title": "A procedure for drug screening without the need to transport urines: use of ion exchange papers and hemagglutination inhibition.", "content": "A procedure was devised for screening for drug abuse in urine specimens by adsorbing the drugs onto papers loaded with ion-exchange resin. The drugs were then eluted from the papers into aqueous saline buffers, which were analyzed by hemagglutination inhibition with antisera specific for morphine, methadone, or barbiturates. The procedure combines the convenience of the ion-exchange papers with the precision and sensitivity of the immunoassay. The preliminary treatment consists of local treatment of urine specimens collected at many distant clinics with ion exchange papers that adsorb 50-65% of alkaloid drugs and 25% of barbiturates and can be shipped, after drying, in simple envelopes by regular mail to a central analytical laboratory for processing. At the central laboratory, portions of specimens are reconstituted in aqueous saline buffers, while drugs from other portions are extracted with solvents at appropriate pH. Drugs are detected in the reconstituted aqueous media by hemagglutination inhibition and spectrophotofluorimetry and confirmed in the solvent extracts by thin-layer chromatography. Recovery of labeled drugs after this treatment and urine screening data showed that the procedure is safe, convenient, and reliable in the case of opiate alkaloids, methadone, amphetamines, and phenothiazine tranquilizers but is less suitable for detection of barbiturates.", "contents": "A procedure for drug screening without the need to transport urines: use of ion exchange papers and hemagglutination inhibition. A procedure was devised for screening for drug abuse in urine specimens by adsorbing the drugs onto papers loaded with ion-exchange resin. The drugs were then eluted from the papers into aqueous saline buffers, which were analyzed by hemagglutination inhibition with antisera specific for morphine, methadone, or barbiturates. The procedure combines the convenience of the ion-exchange papers with the precision and sensitivity of the immunoassay. The preliminary treatment consists of local treatment of urine specimens collected at many distant clinics with ion exchange papers that adsorb 50-65% of alkaloid drugs and 25% of barbiturates and can be shipped, after drying, in simple envelopes by regular mail to a central analytical laboratory for processing. At the central laboratory, portions of specimens are reconstituted in aqueous saline buffers, while drugs from other portions are extracted with solvents at appropriate pH. Drugs are detected in the reconstituted aqueous media by hemagglutination inhibition and spectrophotofluorimetry and confirmed in the solvent extracts by thin-layer chromatography. Recovery of labeled drugs after this treatment and urine screening data showed that the procedure is safe, convenient, and reliable in the case of opiate alkaloids, methadone, amphetamines, and phenothiazine tranquilizers but is less suitable for detection of barbiturates.", "PMID": 954371}
{"id": "pubmed23n0032_1110", "title": "Geriatric primary hyperparathyroidism.", "content": "Primary hyperparathyroidism occurs in a significant number of patients over 60 years of age; about one-third of the 182 patients we studied were in this age group. Primary hyperparathyroidism has been recognized with greater frequency in recent years, particularly in patients without manifestations of renal lithiasis or osteitis fibrosa cystica and in older patients. Age alone is not critical in the occurrence of serious complications from hypercalcemia, the presence of other life-endangering disease, or the development of major postoperative complications in the management of primary hyperparathyroidism. A liberal but selective policy is advocated in advising operation for primary hyperparathyroidism in the elderly. Different clinical patterns of primary hyperparathyroidism are recognized in all age groups. The operative procedure for primary hyperparathyroidism is determined primarily by the surgeons at the time of operation, on the basis of gross characteristics of the parathyroid glands. A precise correlation is not found between microscopic changes, gross changes, and clinical features of primary hyperparathyroidism.", "contents": "Geriatric primary hyperparathyroidism. Primary hyperparathyroidism occurs in a significant number of patients over 60 years of age; about one-third of the 182 patients we studied were in this age group. Primary hyperparathyroidism has been recognized with greater frequency in recent years, particularly in patients without manifestations of renal lithiasis or osteitis fibrosa cystica and in older patients. Age alone is not critical in the occurrence of serious complications from hypercalcemia, the presence of other life-endangering disease, or the development of major postoperative complications in the management of primary hyperparathyroidism. A liberal but selective policy is advocated in advising operation for primary hyperparathyroidism in the elderly. Different clinical patterns of primary hyperparathyroidism are recognized in all age groups. The operative procedure for primary hyperparathyroidism is determined primarily by the surgeons at the time of operation, on the basis of gross characteristics of the parathyroid glands. A precise correlation is not found between microscopic changes, gross changes, and clinical features of primary hyperparathyroidism.", "PMID": 954379}
{"id": "pubmed23n0032_1111", "title": "Systemic consequences of acid-base disorders.", "content": "It is apparent that disturbances in acid-base equilibrium, particularly in the direction of acidosis, interfere with the normal physiologic function of a number of organs. While the effect on each organ system may not be severe, and while adequate compensatory mechanisms may come into play, cummulative involvement of a number of organs, especially when the changes are acute or left untreated, may prove fatal.", "contents": "Systemic consequences of acid-base disorders. It is apparent that disturbances in acid-base equilibrium, particularly in the direction of acidosis, interfere with the normal physiologic function of a number of organs. While the effect on each organ system may not be severe, and while adequate compensatory mechanisms may come into play, cummulative involvement of a number of organs, especially when the changes are acute or left untreated, may prove fatal.", "PMID": 954387}
{"id": "pubmed23n0032_1112", "title": "Cluster headache.", "content": "Cluster headache is a relatively common form of vascular headache. Its characteristic clinical pattern should allow definitive diagnosis in the vast majority of cases. Extensive diagnostic evaluations are not indicated. Treatment is generally unsatisfactory but helpful in a number of cases. It has a limited natural history and is essentially a benign condition.", "contents": "Cluster headache. Cluster headache is a relatively common form of vascular headache. Its characteristic clinical pattern should allow definitive diagnosis in the vast majority of cases. Extensive diagnostic evaluations are not indicated. Treatment is generally unsatisfactory but helpful in a number of cases. It has a limited natural history and is essentially a benign condition.", "PMID": 954391}
{"id": "pubmed23n0032_1113", "title": "A brief program to upgrade the skills of community caregivers.", "content": "This paper describes a program to train community caregivers in mental health-related skills. The goal of the program was to enhance the participants' knowledge of family intervention techniques and to produce more favorable attitudes toward family intervention. A quasi-experimental design was used to evaluate the program and is described in detail.", "contents": "A brief program to upgrade the skills of community caregivers. This paper describes a program to train community caregivers in mental health-related skills. The goal of the program was to enhance the participants' knowledge of family intervention techniques and to produce more favorable attitudes toward family intervention. A quasi-experimental design was used to evaluate the program and is described in detail.", "PMID": 954392}
{"id": "pubmed23n0032_1114", "title": "Unique contributions of churches and clergy to community mental health.", "content": "Good principles and practices of community mental health have been demonstrated by religious communities and leaders within these communities for many years. Using Caplan's model of primary-secondary-tertiary prevention, this paper articulates the unique and cooperative contributions of present-day religious institutions and personnel to the total community mental health endeavor. The validity of church's and clergy's involvement is supported by research data and is consistent with the social-psychological models of conceptualizing emotional problems. Consultation from mental health professionals can aid churches and clergy to actualize even more fully their contributions to community mental health.", "contents": "Unique contributions of churches and clergy to community mental health. Good principles and practices of community mental health have been demonstrated by religious communities and leaders within these communities for many years. Using Caplan's model of primary-secondary-tertiary prevention, this paper articulates the unique and cooperative contributions of present-day religious institutions and personnel to the total community mental health endeavor. The validity of church's and clergy's involvement is supported by research data and is consistent with the social-psychological models of conceptualizing emotional problems. Consultation from mental health professionals can aid churches and clergy to actualize even more fully their contributions to community mental health.", "PMID": 954393}
{"id": "pubmed23n0032_1115", "title": "Planning the phasedown of mental hospitals.", "content": "With impending fiscal shortages limiting the growth of new community mental health services and the ability of mental hospitals to perform contemporary tasks remaining dubious, increased attention is being directed throughout the country to the role of state hospitals. Citizens and professionals in Massachusetts concluded that the needs of individuals now treated in mental hospitals are better met via community-based services as they become available than by services now provided in state hospitals. Consequently, public mental hospitals should be phased down over a 5-year period of time and their funds redeployed to community services. Among the factors affecting the success of this program shift are continuity of care for discharged patients, community acceptance of the mentally ill, and redeployment of personnel to community facilities.", "contents": "Planning the phasedown of mental hospitals. With impending fiscal shortages limiting the growth of new community mental health services and the ability of mental hospitals to perform contemporary tasks remaining dubious, increased attention is being directed throughout the country to the role of state hospitals. Citizens and professionals in Massachusetts concluded that the needs of individuals now treated in mental hospitals are better met via community-based services as they become available than by services now provided in state hospitals. Consequently, public mental hospitals should be phased down over a 5-year period of time and their funds redeployed to community services. Among the factors affecting the success of this program shift are continuity of care for discharged patients, community acceptance of the mentally ill, and redeployment of personnel to community facilities.", "PMID": 954394}
{"id": "pubmed23n0032_1116", "title": "Contracting in crisis intervention.", "content": "The use of contracts in the social services is an important area that needs to be looked at. This paper is concerned specifically with the use of contracts in one particular part of social services: crisis intervention. Contracts that define the working relationship between the client and mental health counselor can be beneficial for both when they attempt to solve the problems that contributed to the crisis situation. There are five benefits to be gained from the use of contracts in crisis intervention and they are briefly discussed.", "contents": "Contracting in crisis intervention. The use of contracts in the social services is an important area that needs to be looked at. This paper is concerned specifically with the use of contracts in one particular part of social services: crisis intervention. Contracts that define the working relationship between the client and mental health counselor can be beneficial for both when they attempt to solve the problems that contributed to the crisis situation. There are five benefits to be gained from the use of contracts in crisis intervention and they are briefly discussed.", "PMID": 954395}
{"id": "pubmed23n0032_1117", "title": "Performance of psychiatric hospital discharges in strict and tolerant environments.", "content": "Community mental health professionals are greatly concerned with the type of social environment most conducive to helping patients remain outside psychiatric institutions and improving the quality of their lives in the community. This paper examines the tolerance of deviance characterizing significant others in the patients' environment as it relates to community tenure and selected measures of performance and quality of life of the older patient in the community. A possible role is suggested for differential tolerance of deviance in the lives of patients discharged from psychiatric hospitals. Although it would appear that patients may return to the hospital at a higher rate from low tolerance environments, it may be that for patients who remain in the community, the quality of life may be better in low tolerance environments in terms of social interaction and life satisfaction. The deviance model is of value in the continuing efforts to understand the role of the social environment in the community life of discharged patients.", "contents": "Performance of psychiatric hospital discharges in strict and tolerant environments. Community mental health professionals are greatly concerned with the type of social environment most conducive to helping patients remain outside psychiatric institutions and improving the quality of their lives in the community. This paper examines the tolerance of deviance characterizing significant others in the patients' environment as it relates to community tenure and selected measures of performance and quality of life of the older patient in the community. A possible role is suggested for differential tolerance of deviance in the lives of patients discharged from psychiatric hospitals. Although it would appear that patients may return to the hospital at a higher rate from low tolerance environments, it may be that for patients who remain in the community, the quality of life may be better in low tolerance environments in terms of social interaction and life satisfaction. The deviance model is of value in the continuing efforts to understand the role of the social environment in the community life of discharged patients.", "PMID": 954396}
{"id": "pubmed23n0032_1118", "title": "The adjunct consumer group.", "content": "Little by little, the community is becoming aware of the Weber Mental Health Center and PAAG. A recent study shows that the community approach leads to high visibility; PAAG activities were known by 37% of 1,000 people recently interviewed. This compares to 5%, 7%, and 15% having knowledge of other more traditional medical centers in Utah. It is felt that this approach is an important part in putting the community mental health center in proper perspective in the community. It is felt that the adjunct consumer group provides almost infinite flexibility to meet human needs. As a result, the Northern Utah Unit of the State Hospital will remain closed, largely because PAAG is serving the community.", "contents": "The adjunct consumer group. Little by little, the community is becoming aware of the Weber Mental Health Center and PAAG. A recent study shows that the community approach leads to high visibility; PAAG activities were known by 37% of 1,000 people recently interviewed. This compares to 5%, 7%, and 15% having knowledge of other more traditional medical centers in Utah. It is felt that this approach is an important part in putting the community mental health center in proper perspective in the community. It is felt that the adjunct consumer group provides almost infinite flexibility to meet human needs. As a result, the Northern Utah Unit of the State Hospital will remain closed, largely because PAAG is serving the community.", "PMID": 954397}
{"id": "pubmed23n0032_1119", "title": "Family therapy research in applied community settings.", "content": "This paper discusses family therapy research in applied settings and highlights those methodological issues relevant to the program evaluator as compared to those relevant to the basic researcher. Four general methodological issues are discussed: design adequacy, issues related to dependent measures, situational factors, and issues of generalization and duration of treatments. Two specific, well-validated applications of family therapy are elaborated; and finally several conclusions are drawn regarding future research strategies.", "contents": "Family therapy research in applied community settings. This paper discusses family therapy research in applied settings and highlights those methodological issues relevant to the program evaluator as compared to those relevant to the basic researcher. Four general methodological issues are discussed: design adequacy, issues related to dependent measures, situational factors, and issues of generalization and duration of treatments. Two specific, well-validated applications of family therapy are elaborated; and finally several conclusions are drawn regarding future research strategies.", "PMID": 954398}
{"id": "pubmed23n0032_1120", "title": "The mental health center's role in school consultation: toward a new model.", "content": "The 1965 Boston Conference on the Education of Psychologists for Community Mental Health recommended a broad movement into the field of education and the development of the consultation function in an array of settings. The coordinator of that conference--at which the term \"community psychology\" was coined--presents a model for extending and redefining the traditional mental health--school consultation model. In this model consultation that is reaction to an existent problem by the clinical \"expert\" is integrated with a comprehensive model of collaboration that moves in the direction of systems change. The concept is presented through a case history of successful school--mental health center collaboration, a documentation of a full consultation service program to a large school system.", "contents": "The mental health center's role in school consultation: toward a new model. The 1965 Boston Conference on the Education of Psychologists for Community Mental Health recommended a broad movement into the field of education and the development of the consultation function in an array of settings. The coordinator of that conference--at which the term \"community psychology\" was coined--presents a model for extending and redefining the traditional mental health--school consultation model. In this model consultation that is reaction to an existent problem by the clinical \"expert\" is integrated with a comprehensive model of collaboration that moves in the direction of systems change. The concept is presented through a case history of successful school--mental health center collaboration, a documentation of a full consultation service program to a large school system.", "PMID": 954399}
{"id": "pubmed23n0032_1121", "title": "Criteria for appropriate and inappropriate referrals to a crisis clinic.", "content": "The purpose of this study was to develop criteria for appropriate and inappropriate referrals by telephone counselors to a crisis clinic. Interviews were conducted with staff members and volunteers of the Suicide Prevention and Crisis Service to explore possible criteria for making a referral. Nine criteria for inappropriate referrals and eight criteria for appropriate referrals were established. Data were then collected from a larger sample of staff members and volunteers (N=56) in the form of a ranked-order questionnaire, and differences between the staff and volunteers were examined. Rankings were significantly different for only 2 of the 17 categories suggesting that there is general agreement between staff and volunteers on the criteria for appropriate and inappropriate referrals for counseling. Policy implications of the accepted criteria are discussed.", "contents": "Criteria for appropriate and inappropriate referrals to a crisis clinic. The purpose of this study was to develop criteria for appropriate and inappropriate referrals by telephone counselors to a crisis clinic. Interviews were conducted with staff members and volunteers of the Suicide Prevention and Crisis Service to explore possible criteria for making a referral. Nine criteria for inappropriate referrals and eight criteria for appropriate referrals were established. Data were then collected from a larger sample of staff members and volunteers (N=56) in the form of a ranked-order questionnaire, and differences between the staff and volunteers were examined. Rankings were significantly different for only 2 of the 17 categories suggesting that there is general agreement between staff and volunteers on the criteria for appropriate and inappropriate referrals for counseling. Policy implications of the accepted criteria are discussed.", "PMID": 954400}
{"id": "pubmed23n0032_1122", "title": "Impact of a personnel workshop on the behavior of support employees toward new patients in a psychiatric outpatient clinic.", "content": "Thirty new patients in a psychiatric outpatient clinic rated the friendliness, interest, politeness, and informativeness of three functionally distinct groups of support employees. Following a personnel workshop, designed to increase the ability to relate to clients and the understanding of patient problems, ratings were obtained from 30 additional new patients. The overall ratings were favorable, both before and after the workshop, for all employee groups on all behavior variables. One employee group received higher ratings than the other two, and the staff members were perceived by the patients as more informative than congenial. The workshop resulted in increased amicability and decreased informativeness.", "contents": "Impact of a personnel workshop on the behavior of support employees toward new patients in a psychiatric outpatient clinic. Thirty new patients in a psychiatric outpatient clinic rated the friendliness, interest, politeness, and informativeness of three functionally distinct groups of support employees. Following a personnel workshop, designed to increase the ability to relate to clients and the understanding of patient problems, ratings were obtained from 30 additional new patients. The overall ratings were favorable, both before and after the workshop, for all employee groups on all behavior variables. One employee group received higher ratings than the other two, and the staff members were perceived by the patients as more informative than congenial. The workshop resulted in increased amicability and decreased informativeness.", "PMID": 954401}
{"id": "pubmed23n0032_1123", "title": "An analysis of contingencies in the Appalachian coalfields.", "content": "The history of the Appalachian coalfields points to the inescapable conclusion that affluence and deprivation alternate according to the demand for coal in the national economy. Although this process has frequently been described, it has never been adequately or comprehensively conceptualized. If, however, the region is viewed in terms of contingencies selecting responses, it is possible to regard the population as operating according to a variable-interval schedule. Implications of such a schedule and recommendations for redesigning the contingencies are made.", "contents": "An analysis of contingencies in the Appalachian coalfields. The history of the Appalachian coalfields points to the inescapable conclusion that affluence and deprivation alternate according to the demand for coal in the national economy. Although this process has frequently been described, it has never been adequately or comprehensively conceptualized. If, however, the region is viewed in terms of contingencies selecting responses, it is possible to regard the population as operating according to a variable-interval schedule. Implications of such a schedule and recommendations for redesigning the contingencies are made.", "PMID": 954402}
{"id": "pubmed23n0032_1124", "title": "A real-time software system on the PDP-11 for two-channel EEG spectral analysis during surgery.", "content": "Three functions are performed in real time by a stand-alone PDP-11-assembly language program. First two channels of EEG data are acquired by quantization techniques; second spectral analysis of the EEG data is carried out; third the resulting spectra are displayed as time compressed spectral profiles on an X-Y plotter. The method provides EEG time trends during anesthesia, permitting evaluation of brain functions.", "contents": "A real-time software system on the PDP-11 for two-channel EEG spectral analysis during surgery. Three functions are performed in real time by a stand-alone PDP-11-assembly language program. First two channels of EEG data are acquired by quantization techniques; second spectral analysis of the EEG data is carried out; third the resulting spectra are displayed as time compressed spectral profiles on an X-Y plotter. The method provides EEG time trends during anesthesia, permitting evaluation of brain functions.", "PMID": 954408}
{"id": "pubmed23n0032_1125", "title": "MUMPS--an economical and efficient time-sharing system for information management.", "content": "MUMPS, the Massachusetts General Hospital Utility MultiProgramming System, is a compact time-sharing system for information management. This paper discusses the historical development of MUMPS and describes the basic modules found in its implementation. Detailed descriptions of the time-sharing executive, MUMPS language interpreter, input/output file system, and global data base are given, emphasizing their integration into an easy-to-use yet powerful system for application program design, development and maintenance.", "contents": "MUMPS--an economical and efficient time-sharing system for information management. MUMPS, the Massachusetts General Hospital Utility MultiProgramming System, is a compact time-sharing system for information management. This paper discusses the historical development of MUMPS and describes the basic modules found in its implementation. Detailed descriptions of the time-sharing executive, MUMPS language interpreter, input/output file system, and global data base are given, emphasizing their integration into an easy-to-use yet powerful system for application program design, development and maintenance.", "PMID": 954409}
{"id": "pubmed23n0032_1126", "title": "Finding condensed descriptions for multi-dimensional data.", "content": "We describe two programs that may be used to find condensed descriptions for data available in a contingency table or in a covariance matrix in the case that these data follow a multinomial or a multivariate normal distribution, respectively. The programs perform a stepwise model search among multiplicative models by computing appropirate likelihood-ratio test statistics.", "contents": "Finding condensed descriptions for multi-dimensional data. We describe two programs that may be used to find condensed descriptions for data available in a contingency table or in a covariance matrix in the case that these data follow a multinomial or a multivariate normal distribution, respectively. The programs perform a stepwise model search among multiplicative models by computing appropirate likelihood-ratio test statistics.", "PMID": 954410}
{"id": "pubmed23n0032_1127", "title": "A discriminant analysis for qualitative data with interactions.", "content": "Two linkable computer programs have been developed for a discriminant analysis with qualitative variables, which permit taking into account 1st order interactions. The first program is used to estimate the parameters required in the second program. The second program allows a classification of new cases as well as an unbaised estimation of error rates.", "contents": "A discriminant analysis for qualitative data with interactions. Two linkable computer programs have been developed for a discriminant analysis with qualitative variables, which permit taking into account 1st order interactions. The first program is used to estimate the parameters required in the second program. The second program allows a classification of new cases as well as an unbaised estimation of error rates.", "PMID": 954411}
{"id": "pubmed23n0032_1128", "title": "Respiratory response of intact Gallus domesticus to sinusoidally varying inhaled carbon dioxide.", "content": "Sinusoidal variations of CO2 were applied to anesthetized White Leghorn cockerels enclosed in a body plethysmograph. Waveforms were 2.5% mean plus 2.5% peak sine wave CO2 in air, at frequencies of 0.011 to 0.467 rad/s. Reflex variation in tidal volume and respiratory interval in response to the inputs of sinusoidally varying fractional content of inhaled CO2 for nine points within the above range of driving frequencies were recorded breath-by-breath. A least squares best-fit procedure was used to estimate mathematical input-output relationships (transfer functions) between the outputs, tidal volume (VT) and respiratory interval (1/f), and the single input, inspired CO2 fraction. For VT, a three time constant model most closely fitted the data. Two time constants, 80 s and 3.9 s, were associated with negligible delay; the third, 14 s, has a 0.35 s time delay. Dynamics of VT and interval reflex responses differed significantly suggesting that they are regulated by separate mechanisms.", "contents": "Respiratory response of intact Gallus domesticus to sinusoidally varying inhaled carbon dioxide. Sinusoidal variations of CO2 were applied to anesthetized White Leghorn cockerels enclosed in a body plethysmograph. Waveforms were 2.5% mean plus 2.5% peak sine wave CO2 in air, at frequencies of 0.011 to 0.467 rad/s. Reflex variation in tidal volume and respiratory interval in response to the inputs of sinusoidally varying fractional content of inhaled CO2 for nine points within the above range of driving frequencies were recorded breath-by-breath. A least squares best-fit procedure was used to estimate mathematical input-output relationships (transfer functions) between the outputs, tidal volume (VT) and respiratory interval (1/f), and the single input, inspired CO2 fraction. For VT, a three time constant model most closely fitted the data. Two time constants, 80 s and 3.9 s, were associated with negligible delay; the third, 14 s, has a 0.35 s time delay. Dynamics of VT and interval reflex responses differed significantly suggesting that they are regulated by separate mechanisms.", "PMID": 954412}
{"id": "pubmed23n0032_1129", "title": "Parametric sensitivity analysis of a homeomorphic model for saccadic and vergence eye movements.", "content": "A non-linear sixth order homeomorphic model, fitted with parameters based on eye movements and physiological data, was tuned so that it provided good simulations for the shapes of the magnitude, velocity and acceleration trajectories. Excellent quantitative agreement was obtained in terms of the Main Sequence diagrams for human eye movements. Parametric sensitivity analysis was done for a saccade of ten degree amplitude, a physiologically normal magnitude at which experimental data is both abundant and relatively noise free. Among the many useful results of this sensitivity analysis are that pulse width (PW) and pulse height (PH) were confirmed as the two controlling parameters for the human eye movement model. Output behavior was relatively insensitive to variations of the passive elements of the plant. This analysis also pointed out that more physiological data are needed to understand the role of the non-linear force-velocity relationship of the extraocular muscles.", "contents": "Parametric sensitivity analysis of a homeomorphic model for saccadic and vergence eye movements. A non-linear sixth order homeomorphic model, fitted with parameters based on eye movements and physiological data, was tuned so that it provided good simulations for the shapes of the magnitude, velocity and acceleration trajectories. Excellent quantitative agreement was obtained in terms of the Main Sequence diagrams for human eye movements. Parametric sensitivity analysis was done for a saccade of ten degree amplitude, a physiologically normal magnitude at which experimental data is both abundant and relatively noise free. Among the many useful results of this sensitivity analysis are that pulse width (PW) and pulse height (PH) were confirmed as the two controlling parameters for the human eye movement model. Output behavior was relatively insensitive to variations of the passive elements of the plant. This analysis also pointed out that more physiological data are needed to understand the role of the non-linear force-velocity relationship of the extraocular muscles.", "PMID": 954413}
{"id": "pubmed23n0032_1130", "title": "Interactive editing of biomedical data.", "content": "Editing options of an interactive graphical-biometry statistical system are described. A method for screening using a highly reduced data file is illustrated. By utilizing properties of sample Fourier coefficients, the problem of component overlap can be resolved. This, in turn, tends to free edited statistical information from the contamination or truncation inherent in the usual screening process.", "contents": "Interactive editing of biomedical data. Editing options of an interactive graphical-biometry statistical system are described. A method for screening using a highly reduced data file is illustrated. By utilizing properties of sample Fourier coefficients, the problem of component overlap can be resolved. This, in turn, tends to free edited statistical information from the contamination or truncation inherent in the usual screening process.", "PMID": 954414}
{"id": "pubmed23n0032_1131", "title": "Digital simulation of the membrane voltages and currents in nerve and muscle fibers.", "content": "This paper describes digital computer simulations of the membrane voltages and currents in axons and muscle fibers. The simulations, which allow calculations ona nonuniform fiber, are based on the discrete Hodgkin-Huxley and Falk-Fatt equations. An efficient algorithm is used to solve the set of equations generated. Calculations are made of the voltage and current at the natural end of a muscle fiber and the results are compared with measurements by other investigators.", "contents": "Digital simulation of the membrane voltages and currents in nerve and muscle fibers. This paper describes digital computer simulations of the membrane voltages and currents in axons and muscle fibers. The simulations, which allow calculations ona nonuniform fiber, are based on the discrete Hodgkin-Huxley and Falk-Fatt equations. An efficient algorithm is used to solve the set of equations generated. Calculations are made of the voltage and current at the natural end of a muscle fiber and the results are compared with measurements by other investigators.", "PMID": 954415}
{"id": "pubmed23n0032_1132", "title": "Instability in a hippocampal neural network.", "content": "Four computer models, HM1-HM4, of a particular hippocampal neural network have been developed. The models represent two cell populations, the pyramidal cells and the basket cells; the populations are coupled so that pyramidal cells are inhibited by activity they excite in the basket cell population. In models HM2-HM4, this recurrent inhibitory pathway contains a temporal dispersion element. Models HM3-HM4 place the pyramidal cells in an additional recurrent excitatory feedback loop. HM4 represents a pair of interacting hippocampal networks. Simulated network responses to single-shock stimulation are presented for various parameter values of the four models. Calculations are extended more than one second following stimulation. Particular attention is given to simulation of network instabilities. Simulated neural activity is discussed in view of experimental work on normal (nonepileptogenic) and epileptogenic hippocampal cortex.", "contents": "Instability in a hippocampal neural network. Four computer models, HM1-HM4, of a particular hippocampal neural network have been developed. The models represent two cell populations, the pyramidal cells and the basket cells; the populations are coupled so that pyramidal cells are inhibited by activity they excite in the basket cell population. In models HM2-HM4, this recurrent inhibitory pathway contains a temporal dispersion element. Models HM3-HM4 place the pyramidal cells in an additional recurrent excitatory feedback loop. HM4 represents a pair of interacting hippocampal networks. Simulated network responses to single-shock stimulation are presented for various parameter values of the four models. Calculations are extended more than one second following stimulation. Particular attention is given to simulation of network instabilities. Simulated neural activity is discussed in view of experimental work on normal (nonepileptogenic) and epileptogenic hippocampal cortex.", "PMID": 954416}
{"id": "pubmed23n0032_1133", "title": "The geometry of the Hodgkin-Huxley Model.", "content": "The Hodgkin-Huxley model for the \"space-clamped\" (i.e., having variables independent of position) squid giant axon is described by a system of four coupled nonlinear ordinary differential equations. In this study, the behavior under various conditions of the solution vector of these equations is predicted qualitatively. This is done by studying the geometry of the phase space of an approximation to the four dimensional system obtained by assuming that the two \"rapid\" components of the system, V and m, are described by algebraic rather than differential equations. Using this method, known properties of the solution of the Hodgkin-Huxley equation, such as threshold, repeated oscillation under constant current stimulus, etc., are explained qualitatively. The direct relation between the Hodgkin-Huxley equations and simpler systems such as the Fitz Hugh equations, which have been used as an aid to the understanding of the Hodgkin-Huxley model, is examined. The roles of the various activation and inactivation components of the Hodgkin-Huxley model are clarified and the effect of modification of those components is studied.", "contents": "The geometry of the Hodgkin-Huxley Model. The Hodgkin-Huxley model for the \"space-clamped\" (i.e., having variables independent of position) squid giant axon is described by a system of four coupled nonlinear ordinary differential equations. In this study, the behavior under various conditions of the solution vector of these equations is predicted qualitatively. This is done by studying the geometry of the phase space of an approximation to the four dimensional system obtained by assuming that the two \"rapid\" components of the system, V and m, are described by algebraic rather than differential equations. Using this method, known properties of the solution of the Hodgkin-Huxley equation, such as threshold, repeated oscillation under constant current stimulus, etc., are explained qualitatively. The direct relation between the Hodgkin-Huxley equations and simpler systems such as the Fitz Hugh equations, which have been used as an aid to the understanding of the Hodgkin-Huxley model, is examined. The roles of the various activation and inactivation components of the Hodgkin-Huxley model are clarified and the effect of modification of those components is studied.", "PMID": 954417}
{"id": "pubmed23n0032_1134", "title": "Comparison of electrical activity and behavioral correlates of neural networks in the cat and locust.", "content": "Extensive experimental data are available on the neural activity and behavioral correlates of specific networks in the locust and in the cat and rabbit. Two networks were selected for comparison, one involving visual movement detector interneurons in the locust and the second a hippocampal network in mammals. Both networks receive inputs correlated with motor activity. Each network was simulated using CSMP, a Continuous Systems Modeling program developed initially by IBM. Signals along the two simulated networks were calculated when similar inputs were applied. In addition, approaches toward relating behavior to neural activity were compared in the two networks.", "contents": "Comparison of electrical activity and behavioral correlates of neural networks in the cat and locust. Extensive experimental data are available on the neural activity and behavioral correlates of specific networks in the locust and in the cat and rabbit. Two networks were selected for comparison, one involving visual movement detector interneurons in the locust and the second a hippocampal network in mammals. Both networks receive inputs correlated with motor activity. Each network was simulated using CSMP, a Continuous Systems Modeling program developed initially by IBM. Signals along the two simulated networks were calculated when similar inputs were applied. In addition, approaches toward relating behavior to neural activity were compared in the two networks.", "PMID": 954418}
{"id": "pubmed23n0032_1135", "title": "Persistent cecal dilatation in a lactating dairy cow.", "content": "Cecal dilatation in cattle is usually manifested by a syndrome of intestinal obstruction requiring surgical intervention. A patient is presented persistent dilatation of the cecum for ten months without evidence of impaired appetite or milk production. The initial clinical signs, progress of the case, and laboratory data are described.", "contents": "Persistent cecal dilatation in a lactating dairy cow. Cecal dilatation in cattle is usually manifested by a syndrome of intestinal obstruction requiring surgical intervention. A patient is presented persistent dilatation of the cecum for ten months without evidence of impaired appetite or milk production. The initial clinical signs, progress of the case, and laboratory data are described.", "PMID": 954438}
{"id": "pubmed23n0032_1136", "title": "Preliminary investigation of the life history of Filaroides hirthi Georgi and Anderson, 1975.", "content": "Filaroides hirthi lungworm infection increased as a function of age among 47, 9 to 32 week old Beagle pups at a commercial breeding establishment. Probit analysis of these data yielded an estimate of 50% infection was by age 17.4 +/- 3.7 weeks. This parasite and/or its associated lesions remained in at least 40% of infected Beagles at least 10 months after exposure to infection had ceased. Reinfection rendered improbable by the nature of the new environment, a laboratory animal colony. Repeated efforts have failed to demonstrate by the Baermann technique eggs or larvae of this parasite in the feces, bronchial mucus, or intact lung tissue of infected Beagles. All efforts to identify an intermediate or alternate definitive hosts have likewise proved unavailing. Piperazines, dichlorvos, thiabendazole, dithiazanine iodide, and levamisole appear to be without effect on the proportion of Beagles infected. The diet, which contained uncooked meat, was fed in a controlled experiment to 10 Beagles from an uninfected source without producing a single case of F. hirthi infection. Life cycles of other members of the superfamily Metastrongyloidea and possible explanations for the non-patency of F. hirthi infection are discussed.", "contents": "Preliminary investigation of the life history of Filaroides hirthi Georgi and Anderson, 1975. Filaroides hirthi lungworm infection increased as a function of age among 47, 9 to 32 week old Beagle pups at a commercial breeding establishment. Probit analysis of these data yielded an estimate of 50% infection was by age 17.4 +/- 3.7 weeks. This parasite and/or its associated lesions remained in at least 40% of infected Beagles at least 10 months after exposure to infection had ceased. Reinfection rendered improbable by the nature of the new environment, a laboratory animal colony. Repeated efforts have failed to demonstrate by the Baermann technique eggs or larvae of this parasite in the feces, bronchial mucus, or intact lung tissue of infected Beagles. All efforts to identify an intermediate or alternate definitive hosts have likewise proved unavailing. Piperazines, dichlorvos, thiabendazole, dithiazanine iodide, and levamisole appear to be without effect on the proportion of Beagles infected. The diet, which contained uncooked meat, was fed in a controlled experiment to 10 Beagles from an uninfected source without producing a single case of F. hirthi infection. Life cycles of other members of the superfamily Metastrongyloidea and possible explanations for the non-patency of F. hirthi infection are discussed.", "PMID": 954439}
{"id": "pubmed23n0032_1137", "title": "Analysis of the microfilarial periodicity of Dirofilaria immitis.", "content": "Microfilarial periodicity displayed by dogs infected with Dirofilaria immitis was analyzed using the methematical model proposed by Sasa and Tanaka (S.E. Asian J. Trop. Med. and Pub. Health 4, 1974, 518-536) except that Pearson's product-moment correlation test was substituted for their Chi-square analysis to estimate goodness of fit. Samples were drawn at 3 hour intervals for periods of 24 or 48 hours in a series of 10 studies conducted on 4 dogs. No significant differences were observed in microfilarial concentration between samples of venous and cutaneous capillary blood. Significant correlation of observed microfilarial densities with the values predicted by the model were observed in 7 of the 10 studies with r2 values ranging from 78.8 to 95.3%. The periodicity index ranged from 28.33 to 53.50 in these 7 studies and thus fell in the subperiodic category. The estimated hour of peak microfilarial density was not confined to the late afternoon as generally believed; for example, values of 04:00 and 22:00 hours were observed. Thus D. immitis microfilaremia cannot accurately be characterized either as diurnal or as nocturnal. Observation of the behavior of the experimental dogs led to an hypothesis that emotional and environmental stresses placed on the host may induce transients in the microfilarial density that may overwhelm or obscure the periodicity function.", "contents": "Analysis of the microfilarial periodicity of Dirofilaria immitis. Microfilarial periodicity displayed by dogs infected with Dirofilaria immitis was analyzed using the methematical model proposed by Sasa and Tanaka (S.E. Asian J. Trop. Med. and Pub. Health 4, 1974, 518-536) except that Pearson's product-moment correlation test was substituted for their Chi-square analysis to estimate goodness of fit. Samples were drawn at 3 hour intervals for periods of 24 or 48 hours in a series of 10 studies conducted on 4 dogs. No significant differences were observed in microfilarial concentration between samples of venous and cutaneous capillary blood. Significant correlation of observed microfilarial densities with the values predicted by the model were observed in 7 of the 10 studies with r2 values ranging from 78.8 to 95.3%. The periodicity index ranged from 28.33 to 53.50 in these 7 studies and thus fell in the subperiodic category. The estimated hour of peak microfilarial density was not confined to the late afternoon as generally believed; for example, values of 04:00 and 22:00 hours were observed. Thus D. immitis microfilaremia cannot accurately be characterized either as diurnal or as nocturnal. Observation of the behavior of the experimental dogs led to an hypothesis that emotional and environmental stresses placed on the host may induce transients in the microfilarial density that may overwhelm or obscure the periodicity function.", "PMID": 954440}
{"id": "pubmed23n0032_1138", "title": "A serological and bacteriological survey of canine brucellosis in Mexico.", "content": "Using agglutination procedures, 203 human and 500 dog sera collected in Mexico City were tested for canine brucellosis. Blood samples from the 500 dogs also were cultured for Brucella canis (B. canis). Positive agglutination titers (1:100 or greater) were found in 27 (13.3%) of the human and 140 (28.0%) of the dog sera tested. B. canis was isolated from the blood of eight dogs. The disease was experimentally produced in susceptible dogs by inoculation with one of the isolated strains.", "contents": "A serological and bacteriological survey of canine brucellosis in Mexico. Using agglutination procedures, 203 human and 500 dog sera collected in Mexico City were tested for canine brucellosis. Blood samples from the 500 dogs also were cultured for Brucella canis (B. canis). Positive agglutination titers (1:100 or greater) were found in 27 (13.3%) of the human and 140 (28.0%) of the dog sera tested. B. canis was isolated from the blood of eight dogs. The disease was experimentally produced in susceptible dogs by inoculation with one of the isolated strains.", "PMID": 954441}
{"id": "pubmed23n0032_1139", "title": "Diaphragmatic hernia in horses.", "content": "The clinical and necropsy findings of three cases of diaphragmatic hernia admitted to Ontario Veterinary College between September 1973 and August 1974 are described. The physical signs of these and other reported cases are discussed, as are methods of diagnosis. Definitive diagnosis is best arrived at by exploratory laparotomy.", "contents": "Diaphragmatic hernia in horses. The clinical and necropsy findings of three cases of diaphragmatic hernia admitted to Ontario Veterinary College between September 1973 and August 1974 are described. The physical signs of these and other reported cases are discussed, as are methods of diagnosis. Definitive diagnosis is best arrived at by exploratory laparotomy.", "PMID": 954442}
{"id": "pubmed23n0032_1140", "title": "Yeasts in bovine semen.", "content": "A total of 129 semen samples from 62 bulls were examined for the presence of mycelial yeasts. Nine species of mycelial yeasts were obtained from the semen; Candida parapsilosis and C. tropicalis were the most frequently isolated organisms. Semen samples from 30 of the bulls had mycelial yeasts present.", "contents": "Yeasts in bovine semen. A total of 129 semen samples from 62 bulls were examined for the presence of mycelial yeasts. Nine species of mycelial yeasts were obtained from the semen; Candida parapsilosis and C. tropicalis were the most frequently isolated organisms. Semen samples from 30 of the bulls had mycelial yeasts present.", "PMID": 954443}
{"id": "pubmed23n0032_1141", "title": "Cuterebra encephalitis in a dog.", "content": "Encephalitis was diagnosed in a 3 1/2-month-old puppy, based on neurological signs, and cerebrospinal fluid examinations. Verminous encephalitis from a migrating Cuterebra sp. larva in the right cerebral hemisphere was confirmed at necropsy.", "contents": "Cuterebra encephalitis in a dog. Encephalitis was diagnosed in a 3 1/2-month-old puppy, based on neurological signs, and cerebrospinal fluid examinations. Verminous encephalitis from a migrating Cuterebra sp. larva in the right cerebral hemisphere was confirmed at necropsy.", "PMID": 954444}
{"id": "pubmed23n0032_1142", "title": "28Mg kinetics in ewes fed normal or tetany prone grass.", "content": "Balance studies and a kinetic evaluation of magnesium metabolism with 28Mg were performed on 6 non pregnant ewes, fed first a young normal dried grass, then frozen tetany prone grass. After changing to the tetany prone grass, there was a small drop in blood Mg and a significant urinary decrease of Mg. Mg balance and endogenous fecal excretion were not significantly modified. Total output of Mg from the pool was reduced. The release of Mg from slow exchanging tissues was very low in tetany prone grass fed ewes. The results suggest that hypomagnesemia in ewes fed tetany prone grass could possibly be related to a shift of body magnesium from blood to some other tissue.", "contents": "28Mg kinetics in ewes fed normal or tetany prone grass. Balance studies and a kinetic evaluation of magnesium metabolism with 28Mg were performed on 6 non pregnant ewes, fed first a young normal dried grass, then frozen tetany prone grass. After changing to the tetany prone grass, there was a small drop in blood Mg and a significant urinary decrease of Mg. Mg balance and endogenous fecal excretion were not significantly modified. Total output of Mg from the pool was reduced. The release of Mg from slow exchanging tissues was very low in tetany prone grass fed ewes. The results suggest that hypomagnesemia in ewes fed tetany prone grass could possibly be related to a shift of body magnesium from blood to some other tissue.", "PMID": 954445}
{"id": "pubmed23n0032_1143", "title": "A comparison of aphasic and non-brain-injured adults on a dichotic CV-syllable listening task.", "content": "A dichotic CV-syllable listening task was administered to a group of eleven non-brain-injured adults and to a group of eleven adult aphasics. The results of this study may be summarized as follows: 1)The group of non-brain-injured adults showed a slight right ear advantage for dichotically presented CV-syllables. 2)In comparison with the control group the asphasic group showed a bilateral deficit in response to the dichotic CV-syllables, superimposed on a non-significant right ear advantage. 3) The asphasic group demonstrated a great deal of intersubject variability on the dichotic task with six aphasics showing a right ear preference for the stimuli. The non-brain-injured subjects performed more homogeneously on the task. 4) The two subgroups of aphasics, a right ear advantage group and a left ear advantage group, performed significantly different on the dichotic listening task. 5) Single correct data analysis proved valuable by deleting accuracy of report for an examination of trials in which there was true competition for the single left hemispheric speech processor. These results were analyzed in terms of a functional model of auditory processing. In view of this model, the bilateral deficit in dichotic performance of the asphasic group was accounted for by the presence of a lesion within the dominant left hemisphere, where the speech signals from both ears converge for final processing. The right ear advantage shown by one asphasic subgroup was explained by a lesion interfering with the corpus callosal pathways from the left hemisphere; the left ear advantage observed within the other subgroup was explained by a lesion in the area of the auditory processor of the left hemisphere.", "contents": "A comparison of aphasic and non-brain-injured adults on a dichotic CV-syllable listening task. A dichotic CV-syllable listening task was administered to a group of eleven non-brain-injured adults and to a group of eleven adult aphasics. The results of this study may be summarized as follows: 1)The group of non-brain-injured adults showed a slight right ear advantage for dichotically presented CV-syllables. 2)In comparison with the control group the asphasic group showed a bilateral deficit in response to the dichotic CV-syllables, superimposed on a non-significant right ear advantage. 3) The asphasic group demonstrated a great deal of intersubject variability on the dichotic task with six aphasics showing a right ear preference for the stimuli. The non-brain-injured subjects performed more homogeneously on the task. 4) The two subgroups of aphasics, a right ear advantage group and a left ear advantage group, performed significantly different on the dichotic listening task. 5) Single correct data analysis proved valuable by deleting accuracy of report for an examination of trials in which there was true competition for the single left hemispheric speech processor. These results were analyzed in terms of a functional model of auditory processing. In view of this model, the bilateral deficit in dichotic performance of the asphasic group was accounted for by the presence of a lesion within the dominant left hemisphere, where the speech signals from both ears converge for final processing. The right ear advantage shown by one asphasic subgroup was explained by a lesion interfering with the corpus callosal pathways from the left hemisphere; the left ear advantage observed within the other subgroup was explained by a lesion in the area of the auditory processor of the left hemisphere.", "PMID": 954446}
{"id": "pubmed23n0032_1144", "title": "Aphasics' sentence repetition behavior as a funciton of grammaticality.", "content": "Twenty asphasics repeated grammatical and ungrammatical sentences. Ungrammatical sentences were characterized by violations of syntactic and/or semantic structure. Aphasics repeated accurately more grammatical than ungrammatical sentences. Ungrammatical sentences with violations of syntactic structure were repeated less accurately than those with preserved syntax. Aphasics' repetition errors were classified as: incorrect repetition, inapropriate correction, morphological error, lexical deletion and substitution responses. Repetition errors appeared to result from performance deficits, such as reduced retention span and physiological limitations of the speech musculature. Results suggest that asphasics are to some extent guided by a greater residual linguistic knowledge or competence than might be inferred from their spontaneous production.", "contents": "Aphasics' sentence repetition behavior as a funciton of grammaticality. Twenty asphasics repeated grammatical and ungrammatical sentences. Ungrammatical sentences were characterized by violations of syntactic and/or semantic structure. Aphasics repeated accurately more grammatical than ungrammatical sentences. Ungrammatical sentences with violations of syntactic structure were repeated less accurately than those with preserved syntax. Aphasics' repetition errors were classified as: incorrect repetition, inapropriate correction, morphological error, lexical deletion and substitution responses. Repetition errors appeared to result from performance deficits, such as reduced retention span and physiological limitations of the speech musculature. Results suggest that asphasics are to some extent guided by a greater residual linguistic knowledge or competence than might be inferred from their spontaneous production.", "PMID": 954447}
{"id": "pubmed23n0032_1145", "title": "Personality factors in jargon aphasia.", "content": "The personality background of 20 patients with jargon aphasia was compared to that of a standard aphasia group with particular reference to features of the so called anosognosic personality; denial or marked overt fear of illness and strong work orientation. Such attitudes toward illness were found in 19 of 20 jargon subjects, and seven of the comparison group. Nineteen of the jargon patients and eleven of the non-jargon asphasics were described as highly work oriented. The premorbid personality was also significantly related to the type of denial of the speech deficit, and to related aspects of behavior. Patients with strong premorbid denial explicitly and completely denied their asphasic deficits, and were affable, bland or unconcerned. Patients with a history of overt fear of illness had implicit or mixed forms of denial, and showed disturbed, agitated, paranoid and often psychotic behavior. The type of denial is thus related not only to premorbid personality, but to observable behavior in hospital. The observations are regarded as providing additional evidence for the view that jargon may be regarded as \"anosognosic aphasia,\" and represents not only a linguistic deficit, but an adaptation to the deficit.", "contents": "Personality factors in jargon aphasia. The personality background of 20 patients with jargon aphasia was compared to that of a standard aphasia group with particular reference to features of the so called anosognosic personality; denial or marked overt fear of illness and strong work orientation. Such attitudes toward illness were found in 19 of 20 jargon subjects, and seven of the comparison group. Nineteen of the jargon patients and eleven of the non-jargon asphasics were described as highly work oriented. The premorbid personality was also significantly related to the type of denial of the speech deficit, and to related aspects of behavior. Patients with strong premorbid denial explicitly and completely denied their asphasic deficits, and were affable, bland or unconcerned. Patients with a history of overt fear of illness had implicit or mixed forms of denial, and showed disturbed, agitated, paranoid and often psychotic behavior. The type of denial is thus related not only to premorbid personality, but to observable behavior in hospital. The observations are regarded as providing additional evidence for the view that jargon may be regarded as \"anosognosic aphasia,\" and represents not only a linguistic deficit, but an adaptation to the deficit.", "PMID": 954448}
{"id": "pubmed23n0032_1146", "title": "The \"tip-of-the-tongue\" phenomenon in aphasia.", "content": "Forty-two male aphasics, classified as Broc's, Wernicke's, anomic, and conduction, were examined for their ability to partially retrieve acoustic features of words they could not provide in a confrontation naming task (the \"tip-of-the-tongue\" phenomenon). The results indicated that conduction aphasics were superior to Wernicke's and anomic asphasics in their ability to identify both the first letter and the syllabic length of the words they could not name. Since the aphasic groups were comparable in their naming difficulty, the findings could not be attributed to degree of wordfinding impairment. The difference between groups that were obtained using the TOT technique are discussed with regard to differences in the word finding process.", "contents": "The \"tip-of-the-tongue\" phenomenon in aphasia. Forty-two male aphasics, classified as Broc's, Wernicke's, anomic, and conduction, were examined for their ability to partially retrieve acoustic features of words they could not provide in a confrontation naming task (the \"tip-of-the-tongue\" phenomenon). The results indicated that conduction aphasics were superior to Wernicke's and anomic asphasics in their ability to identify both the first letter and the syllabic length of the words they could not name. Since the aphasic groups were comparable in their naming difficulty, the findings could not be attributed to degree of wordfinding impairment. The difference between groups that were obtained using the TOT technique are discussed with regard to differences in the word finding process.", "PMID": 954449}
{"id": "pubmed23n0032_1147", "title": "[A case of bilateral lesions in the temporal convexity: attempt to define symptoms].", "content": "The case is reported of a boy aged 15 who suffered a bilateral lesion of the temporal convexity after a brain traumatism; he died 5 years later and the symptoms during that period are described. The clinical picture is adequately described neither under the title of dementia, nor under that of aphasia. The concept of an abolition involving the systems of cultural mediation (linguistic, technical) is introduced. The status of echolalia and echo-praxia is discussed.", "contents": "[A case of bilateral lesions in the temporal convexity: attempt to define symptoms]. The case is reported of a boy aged 15 who suffered a bilateral lesion of the temporal convexity after a brain traumatism; he died 5 years later and the symptoms during that period are described. The clinical picture is adequately described neither under the title of dementia, nor under that of aphasia. The concept of an abolition involving the systems of cultural mediation (linguistic, technical) is introduced. The status of echolalia and echo-praxia is discussed.", "PMID": 954450}
{"id": "pubmed23n0032_1148", "title": "Short-term recognition memory in relation to severity of head injury.", "content": "Short-term recognition memory for random shapes was studied in 24 patients with head injury. The severity of head injury as indexed by duration of coma was closely related to impairment in performance. Disruption of short-term recognition memory was associated with neurologic deficit, asphasic disturbance, and signs of brain stem involvement. Performance was not related to association value of the shapes irrespective of linguistic competence. The findings are discussed in relation to recent studies of continuous recognition memory after head injury and hypotheses concerning neurological dysfunction in head injury.", "contents": "Short-term recognition memory in relation to severity of head injury. Short-term recognition memory for random shapes was studied in 24 patients with head injury. The severity of head injury as indexed by duration of coma was closely related to impairment in performance. Disruption of short-term recognition memory was associated with neurologic deficit, asphasic disturbance, and signs of brain stem involvement. Performance was not related to association value of the shapes irrespective of linguistic competence. The findings are discussed in relation to recent studies of continuous recognition memory after head injury and hypotheses concerning neurological dysfunction in head injury.", "PMID": 954452}
{"id": "pubmed23n0032_1149", "title": "The coin lesion story: update 1976. Twenty years' experience with thoracotomy for 179 suspected malignant coin lesions.", "content": "We reviewed 179 patients who had undergone thoracotomy and resection of a suspected malignant coin lesion of the lung over the past 20 years to see if a policy of early thoracotomy was therapeutically valid. The average diameter of all lesions was 1.6 cm; the average diameter of 27 malignant lesions (15 percent) was 1.8 cm. Follow-up of the 27 patients with malignant neoplasms was 100 percent. The present survival rate of the 19 patients with primary lung cancer is 89 percent (17/19). Of 12 cases of primary lung cancer followed for five years, ten (83 percent) survived. The five-year survival of the eight patients with metastatic lesions was 25 percent (2/8). There were no postoperative deaths and few serious postoperative complications (four patients or 2 percent). Very small primary lung cancers detected and treated early do have the same poor prognosis as larger primary cancers.", "contents": "The coin lesion story: update 1976. Twenty years' experience with thoracotomy for 179 suspected malignant coin lesions. We reviewed 179 patients who had undergone thoracotomy and resection of a suspected malignant coin lesion of the lung over the past 20 years to see if a policy of early thoracotomy was therapeutically valid. The average diameter of all lesions was 1.6 cm; the average diameter of 27 malignant lesions (15 percent) was 1.8 cm. Follow-up of the 27 patients with malignant neoplasms was 100 percent. The present survival rate of the 19 patients with primary lung cancer is 89 percent (17/19). Of 12 cases of primary lung cancer followed for five years, ten (83 percent) survived. The five-year survival of the eight patients with metastatic lesions was 25 percent (2/8). There were no postoperative deaths and few serious postoperative complications (four patients or 2 percent). Very small primary lung cancers detected and treated early do have the same poor prognosis as larger primary cancers.", "PMID": 954458}
{"id": "pubmed23n0032_1150", "title": "Clavicular Auscultation. Preferential clavicular transmission and amplification of aortic valve murmurs.", "content": "Clavicular transmission of aortic valve murmurs was investigated in 15 consecutive patients. In all patients, transmission to the clavicle of aortic systolic murmurs was detected both by auscultation and by phonocardiographic studies. Of seven patients with aortic diastolic murmurs, auscultation revealed clavicular transmission in six, but only four were recordable. There was increased murmur amplitude over the clavicle in 13 out of 15 cases. In contrast, murmurs were attenuated over the subclavian artery in eight out of nine and over the carotid artery in nine out of 11 patients. We conclude that clavicular auscultation regularly discloses aortic valve murmurs. The aortic systolic murmurs are consistently propagated to the clavicle, where they are usually conspicuously amplified. Aortic systolic murmurs are less frequently transmitted to the carotid and subclavian arteries, where they are usually considerably attenuated. Clavicular auscultation appears to be more rewarding than the traditional search for transmission of aortic murmurs to the carotid artery.", "contents": "Clavicular Auscultation. Preferential clavicular transmission and amplification of aortic valve murmurs. Clavicular transmission of aortic valve murmurs was investigated in 15 consecutive patients. In all patients, transmission to the clavicle of aortic systolic murmurs was detected both by auscultation and by phonocardiographic studies. Of seven patients with aortic diastolic murmurs, auscultation revealed clavicular transmission in six, but only four were recordable. There was increased murmur amplitude over the clavicle in 13 out of 15 cases. In contrast, murmurs were attenuated over the subclavian artery in eight out of nine and over the carotid artery in nine out of 11 patients. We conclude that clavicular auscultation regularly discloses aortic valve murmurs. The aortic systolic murmurs are consistently propagated to the clavicle, where they are usually conspicuously amplified. Aortic systolic murmurs are less frequently transmitted to the carotid and subclavian arteries, where they are usually considerably attenuated. Clavicular auscultation appears to be more rewarding than the traditional search for transmission of aortic murmurs to the carotid artery.", "PMID": 954459}
{"id": "pubmed23n0032_1151", "title": "A test of the practical value of estimating breath sound intensity. Breath sounds related to measured ventilatory function.", "content": "Each of four examiners performed standardized physical examinations on a group of patients who had just undergone tests of ventilatory function. The intensity of breath sounds heard with deep inspiration was graded on a rating scale of 0 to 4; the grades in six areas of the chest were added to give a total score, with possible values ranging from 0 to 24. Correlation of breath-sound scores with percentage of predicted forced expiratory volume in one second (FEV1) was significant at the 1 percent level for all of the examiners. Differences between the examiners in their assessment of breath sounds were not statistically significant. Grading the loudness of breath sounds was a poor screening test for mild ventilatory abnormality, but normal breath sounds nearly excluded the possibility of severe reduction in the FEV1. Definitely reduced breath-sound intensity was strong evidence for the presence of obstructive pulmonary disease.", "contents": "A test of the practical value of estimating breath sound intensity. Breath sounds related to measured ventilatory function. Each of four examiners performed standardized physical examinations on a group of patients who had just undergone tests of ventilatory function. The intensity of breath sounds heard with deep inspiration was graded on a rating scale of 0 to 4; the grades in six areas of the chest were added to give a total score, with possible values ranging from 0 to 24. Correlation of breath-sound scores with percentage of predicted forced expiratory volume in one second (FEV1) was significant at the 1 percent level for all of the examiners. Differences between the examiners in their assessment of breath sounds were not statistically significant. Grading the loudness of breath sounds was a poor screening test for mild ventilatory abnormality, but normal breath sounds nearly excluded the possibility of severe reduction in the FEV1. Definitely reduced breath-sound intensity was strong evidence for the presence of obstructive pulmonary disease.", "PMID": 954460}
{"id": "pubmed23n0032_1152", "title": "The prognosis of sarcoidosis in children.", "content": "Of 28 children with sarcoidosis, who have a mean follow-up period of approximately nine years, five (18 percent) have sustained severe damage from the disease. Two of these patients suffer from blindness, and three others have severe restrictive pulmonary disease. There has so far been no fatality. The presence of cutaneous lesions suggests a guarded prognosis, but the effectiveness of corticosteroid therapy cannot be accurately determined. The degree of pulmonary involvement evident on the chest x-ray film at the time of diagnosis is not always indicative of the ultimate prognosis.", "contents": "The prognosis of sarcoidosis in children. Of 28 children with sarcoidosis, who have a mean follow-up period of approximately nine years, five (18 percent) have sustained severe damage from the disease. Two of these patients suffer from blindness, and three others have severe restrictive pulmonary disease. There has so far been no fatality. The presence of cutaneous lesions suggests a guarded prognosis, but the effectiveness of corticosteroid therapy cannot be accurately determined. The degree of pulmonary involvement evident on the chest x-ray film at the time of diagnosis is not always indicative of the ultimate prognosis.", "PMID": 954461}
{"id": "pubmed23n0032_1153", "title": "Echocardiographic diagnosis of double-outlet right ventricle.", "content": "An echocardiographic technique (V scan) for confirming the diagnosis of double-outlet right ventricle is described. The technique demonstrates a frequent pathologic feature of double-outlet right ventricle, ie, origin in both great arteries side by side and anterior to the ventricular septum. The V scan offers greater diagnostic accuracy than the mere demonstration of mitral-semilunar valvular discontinuity.", "contents": "Echocardiographic diagnosis of double-outlet right ventricle. An echocardiographic technique (V scan) for confirming the diagnosis of double-outlet right ventricle is described. The technique demonstrates a frequent pathologic feature of double-outlet right ventricle, ie, origin in both great arteries side by side and anterior to the ventricular septum. The V scan offers greater diagnostic accuracy than the mere demonstration of mitral-semilunar valvular discontinuity.", "PMID": 954462}
{"id": "pubmed23n0032_1154", "title": "Value of serial apexcardiograms during and after myocardial infarction.", "content": "Apexcardiograms were systematically recorded serially in 40 patients after acute myocardial infarction, in an additional 21 patients with proven aneurysms, and in 18 patients with good ventricular performance following myocardial infarction. Abnormalities of the \"A\" wave and systolic waves of the apexcardiogram correlated well with akinesia or dyskinesia proven by cinefluoroscopic and cineangiographic studies. Third and fourth heart sounds and apical systolic murmurs also correlated well with evidence of ventricular dysfunction. This study indicates that the apexcardiogram is a good qualitative tool for serially assessing patients following acute myocardial infarction and that the results on invasive study may be predicted in most patients.", "contents": "Value of serial apexcardiograms during and after myocardial infarction. Apexcardiograms were systematically recorded serially in 40 patients after acute myocardial infarction, in an additional 21 patients with proven aneurysms, and in 18 patients with good ventricular performance following myocardial infarction. Abnormalities of the \"A\" wave and systolic waves of the apexcardiogram correlated well with akinesia or dyskinesia proven by cinefluoroscopic and cineangiographic studies. Third and fourth heart sounds and apical systolic murmurs also correlated well with evidence of ventricular dysfunction. This study indicates that the apexcardiogram is a good qualitative tool for serially assessing patients following acute myocardial infarction and that the results on invasive study may be predicted in most patients.", "PMID": 954463}
{"id": "pubmed23n0032_1155", "title": "Multiple lesions of the conduction system in a case of cardiac rhabdomyosarcoma with complex arrhythmias. An anatomic and clinical study.", "content": "Anatomic and electrocardiographic correlations in a case of primary cardiac rhabdomyosarcoma are examined. Interatrial and atrioventricular conduction disturbances were associated with multiple lesions involving the alleged internodal pathways, together with atrial-atrioventricular nodal connections. The clinicopathologic findings seem to be consistent with the hypotheses of the functional and morphologic value of Bachmann's fascicle, and of the inherent pathways of interatrial and internodal conduction in health and disease.", "contents": "Multiple lesions of the conduction system in a case of cardiac rhabdomyosarcoma with complex arrhythmias. An anatomic and clinical study. Anatomic and electrocardiographic correlations in a case of primary cardiac rhabdomyosarcoma are examined. Interatrial and atrioventricular conduction disturbances were associated with multiple lesions involving the alleged internodal pathways, together with atrial-atrioventricular nodal connections. The clinicopathologic findings seem to be consistent with the hypotheses of the functional and morphologic value of Bachmann's fascicle, and of the inherent pathways of interatrial and internodal conduction in health and disease.", "PMID": 954464}
{"id": "pubmed23n0032_1156", "title": "Limited form of Wegener's granulomatosis.", "content": "A 40-year-old man had a pathologically proved limited form of Wegener's granulomatosis complicating recurrent and massive hemoptysis from his cavitary pulmonary lesion. Of significant interest and clinical importance is the unusual location of the patient's pulmonary lesion, occurring in the posterior segment of the right upper lobe. This experience demonstrates that the location of the pulmonary nodule is not necessarily useful in distinguishing Wegener's granulomatosis from infectious granulomatosis, particularly tuberculosis.", "contents": "Limited form of Wegener's granulomatosis. A 40-year-old man had a pathologically proved limited form of Wegener's granulomatosis complicating recurrent and massive hemoptysis from his cavitary pulmonary lesion. Of significant interest and clinical importance is the unusual location of the patient's pulmonary lesion, occurring in the posterior segment of the right upper lobe. This experience demonstrates that the location of the pulmonary nodule is not necessarily useful in distinguishing Wegener's granulomatosis from infectious granulomatosis, particularly tuberculosis.", "PMID": 954466}
{"id": "pubmed23n0032_1157", "title": "Pulmonary botryomycoma.", "content": "A patient with a mobile mass of anaerobic organisms in the pulmonary parenchyma is presented. Radiographically it resembled a fungous ball. This observed botryomycoma may be part of the spectrum of aspiration pneumonia.", "contents": "Pulmonary botryomycoma. A patient with a mobile mass of anaerobic organisms in the pulmonary parenchyma is presented. Radiographically it resembled a fungous ball. This observed botryomycoma may be part of the spectrum of aspiration pneumonia.", "PMID": 954467}
{"id": "pubmed23n0032_1158", "title": "Recurrent ventricular tachycardia due to reentry within the bundle branches.", "content": "A case of recurrent ventricular paroxysmal tachycardia in a young man without obvious heart disease is described. Posttachycardiac T-wave inversion followed the arrhythmic episodes. Programmed stimulation of the heart in association with intracardiac recordings showed evidence in favor of reentry within the bundle branches and its divisions during tachycardia. A critical prolongation of the H2-V3 interval preceded each paroxysm of tachycardia.", "contents": "Recurrent ventricular tachycardia due to reentry within the bundle branches. A case of recurrent ventricular paroxysmal tachycardia in a young man without obvious heart disease is described. Posttachycardiac T-wave inversion followed the arrhythmic episodes. Programmed stimulation of the heart in association with intracardiac recordings showed evidence in favor of reentry within the bundle branches and its divisions during tachycardia. A critical prolongation of the H2-V3 interval preceded each paroxysm of tachycardia.", "PMID": 954468}
{"id": "pubmed23n0032_1159", "title": "Modified exclusion technique for complicated esophageal perforation.", "content": "A case of complicated spontaneous esophageal perforation is presented. The control of gastroesophageal reflux by esophageal banding appears to be a crucial factor in healing. Using a modified esophageal exclusion technique without sacrifice of the esophagus proves to be both effective and lifesaving.", "contents": "Modified exclusion technique for complicated esophageal perforation. A case of complicated spontaneous esophageal perforation is presented. The control of gastroesophageal reflux by esophageal banding appears to be a crucial factor in healing. Using a modified esophageal exclusion technique without sacrifice of the esophagus proves to be both effective and lifesaving.", "PMID": 954469}
{"id": "pubmed23n0032_1160", "title": "Prophylactic irradiation of the lung in Ewing's sarcoma.", "content": "A patient with Ewing's sarcoma of the left scapula received prophylactic radiotherapy to the left lung following wide excision of the tumor. While the left lung remained free of tumor, there was metastatic invasion of the right lung within ten months of the initial diagnosis of the disease. Since Ewing's sarcoma frequently metastasizes to the lungs, it is suggested that prophylactic irradiation of the lung may be of value in the prevention of metastases.", "contents": "Prophylactic irradiation of the lung in Ewing's sarcoma. A patient with Ewing's sarcoma of the left scapula received prophylactic radiotherapy to the left lung following wide excision of the tumor. While the left lung remained free of tumor, there was metastatic invasion of the right lung within ten months of the initial diagnosis of the disease. Since Ewing's sarcoma frequently metastasizes to the lungs, it is suggested that prophylactic irradiation of the lung may be of value in the prevention of metastases.", "PMID": 954470}
{"id": "pubmed23n0032_1161", "title": "Pulmonary varices. Report of two cases and review of the literature.", "content": "Two cases of pulmonary varices are presented. The lesion is rare, and only 32 cases have been reported in the literature. Although the lesion is benign in nature, complications are possible, and death has occurred from rupture of the varix intrapleurally or into the bronchial tree.", "contents": "Pulmonary varices. Report of two cases and review of the literature. Two cases of pulmonary varices are presented. The lesion is rare, and only 32 cases have been reported in the literature. Although the lesion is benign in nature, complications are possible, and death has occurred from rupture of the varix intrapleurally or into the bronchial tree.", "PMID": 954471}
{"id": "pubmed23n0032_1162", "title": "Striking electrocardiographic changes associated with pheochromocytoma. Masquerading as ischemic heart disease.", "content": "A patient with pheochromocytoma presented striking electrocardiographic changes mimicking ischemic heart disease at one time and acute pulmonary embolism at other times. Diffuse left ventricular hypokinesia was demonstrated in the presence of normal coronary arteries. Following removal of the pheochromocytoma, the electrocardiographic abnormalities disappeared.", "contents": "Striking electrocardiographic changes associated with pheochromocytoma. Masquerading as ischemic heart disease. A patient with pheochromocytoma presented striking electrocardiographic changes mimicking ischemic heart disease at one time and acute pulmonary embolism at other times. Diffuse left ventricular hypokinesia was demonstrated in the presence of normal coronary arteries. Following removal of the pheochromocytoma, the electrocardiographic abnormalities disappeared.", "PMID": 954472}
{"id": "pubmed23n0032_1163", "title": "Echocardiographic diagnosis of thrombus on a heterograft aortic valve in the mitral position.", "content": "Echocardiographic studies were used to diagnose a thrombus adherent to the ventricular surface of a stent-mounted porcine aortic heterograft valve in the mitral position. Multiple, intense, irregularly thickened echoes were present within and behind the anterior and posterior heterograft valvular stent. This suggested the presence of adherent biologic material, such as a thrombus. The abnormal echocardiographic findings are compared to the findings in a normally functioning porcine heterograft aortic valve in the mitral position.", "contents": "Echocardiographic diagnosis of thrombus on a heterograft aortic valve in the mitral position. Echocardiographic studies were used to diagnose a thrombus adherent to the ventricular surface of a stent-mounted porcine aortic heterograft valve in the mitral position. Multiple, intense, irregularly thickened echoes were present within and behind the anterior and posterior heterograft valvular stent. This suggested the presence of adherent biologic material, such as a thrombus. The abnormal echocardiographic findings are compared to the findings in a normally functioning porcine heterograft aortic valve in the mitral position.", "PMID": 954473}
{"id": "pubmed23n0032_1164", "title": "Sudden disappearance of electrocardiographic pattern of anteroseptal myocardial infarction. Result of superimposed acute posterior myocardial infarction.", "content": "In a 76-year-old man an electrocardiographic pattern of acute anteroseptal myocardial infarction disappeared suudenly. At necropsy, a more recent posterior myocardial infarct was found, in addition to an acute anteroseptal infarct. \"Normalization\" of the electrocardiogram from the pattern of anteroseptal myocardial infarction in this case resulted from the loss of opposing electromotive forces in the posterior wall because of posterior infarction.", "contents": "Sudden disappearance of electrocardiographic pattern of anteroseptal myocardial infarction. Result of superimposed acute posterior myocardial infarction. In a 76-year-old man an electrocardiographic pattern of acute anteroseptal myocardial infarction disappeared suudenly. At necropsy, a more recent posterior myocardial infarct was found, in addition to an acute anteroseptal infarct. \"Normalization\" of the electrocardiogram from the pattern of anteroseptal myocardial infarction in this case resulted from the loss of opposing electromotive forces in the posterior wall because of posterior infarction.", "PMID": 954474}
{"id": "pubmed23n0032_1165", "title": "Influence of immunosuppression on the action of antimycobacterial drugs in experimental tuberculosis. I. Effects of isoniazid, rifampicin and streptomycin on the survival of tuberculous mice immunosuppressed by azathioprine (Imuran).", "content": "The influence of the host immunosuppression on antimycobacterial effects of isoniazid, rifampicin or streptomycin was studied on animal models. Differences in survival rates of mice influenced or uninfluenced by azathioprine (Imuran) and exposed to six applications of antituberculous drugs (given once a day in the monotherapy) were statistically evaluated. The dose-dependent antimycobacterial effects of rifampicin or isoniazid were not significantly altered by the immunosuppression of the host. In contrary to that definite dose-dependent effect of streptomycin, observed in azathioprine-uninfluenced mice, cannot be confirmed in immunosuppressed mice in which no effect of streptomycin on the survival of tuberculous mice could be estimated.", "contents": "Influence of immunosuppression on the action of antimycobacterial drugs in experimental tuberculosis. I. Effects of isoniazid, rifampicin and streptomycin on the survival of tuberculous mice immunosuppressed by azathioprine (Imuran). The influence of the host immunosuppression on antimycobacterial effects of isoniazid, rifampicin or streptomycin was studied on animal models. Differences in survival rates of mice influenced or uninfluenced by azathioprine (Imuran) and exposed to six applications of antituberculous drugs (given once a day in the monotherapy) were statistically evaluated. The dose-dependent antimycobacterial effects of rifampicin or isoniazid were not significantly altered by the immunosuppression of the host. In contrary to that definite dose-dependent effect of streptomycin, observed in azathioprine-uninfluenced mice, cannot be confirmed in immunosuppressed mice in which no effect of streptomycin on the survival of tuberculous mice could be estimated.", "PMID": 954481}
{"id": "pubmed23n0032_1166", "title": "Treatment of human leptospira infections with semicillin (ampicillin) or with amoxil (amoxycillin).", "content": "Ampicillin (Semicillin) and Amoxycillin (Amoxil) have a strong leptospirocidal effect in vitro and also in vivo. We have tested these drugs on 28 patients suffering from Leptospira infection. The patients generally received per os 3 g Ampicillin (Semicillin) or 2 g Amoxycillin (Amoxil) per day over a period of 6 days. In the groups treated with Ampicillin and Amoxycillin the average durations of fever during the treatment were 1.6 and 1.2 days, respectively. In the first group (Ampicillin) a 'second wave of fever' occurred in one case (5%) and in the second group (Amoxycillin), in 0% (as compared to 22% after treatment with benzylpenicillin). On the basis of our experiences, Ampicillin and Amoxycillin are strongly recommended for the treatment of human Leptospira infections.", "contents": "Treatment of human leptospira infections with semicillin (ampicillin) or with amoxil (amoxycillin). Ampicillin (Semicillin) and Amoxycillin (Amoxil) have a strong leptospirocidal effect in vitro and also in vivo. We have tested these drugs on 28 patients suffering from Leptospira infection. The patients generally received per os 3 g Ampicillin (Semicillin) or 2 g Amoxycillin (Amoxil) per day over a period of 6 days. In the groups treated with Ampicillin and Amoxycillin the average durations of fever during the treatment were 1.6 and 1.2 days, respectively. In the first group (Ampicillin) a 'second wave of fever' occurred in one case (5%) and in the second group (Amoxycillin), in 0% (as compared to 22% after treatment with benzylpenicillin). On the basis of our experiences, Ampicillin and Amoxycillin are strongly recommended for the treatment of human Leptospira infections.", "PMID": 954482}
{"id": "pubmed23n0032_1167", "title": "Distribution of sulfonamides and sulfonamide potentiators between red blood cells, proteins and aqueous phases of the blood of different species.", "content": "The uptake of sulfonamides and sulfonamide potentiators by plasma albumin, red blood cells and hemoglobin of man, ox, rabbit and mouse has been determined. From the figures obtained the distribution of these compounds between cells, macromolecules and aqueous phases of blood has been calculated. In most cases more than 50% of a total sulfonamide in blood is bound to plasma albumin. The time necessary for establishment of concentration equilibrium of the drugs investigated between erythrocytes and surrounding medium varies between a few seconds and several minutes, differences between the species being encountered. Hemoglobin binding of the drugs is much smaller than albumin binding. Nevertheless, drug concentration within the erythrocytes is generally higher than in the surrounding medium.", "contents": "Distribution of sulfonamides and sulfonamide potentiators between red blood cells, proteins and aqueous phases of the blood of different species. The uptake of sulfonamides and sulfonamide potentiators by plasma albumin, red blood cells and hemoglobin of man, ox, rabbit and mouse has been determined. From the figures obtained the distribution of these compounds between cells, macromolecules and aqueous phases of blood has been calculated. In most cases more than 50% of a total sulfonamide in blood is bound to plasma albumin. The time necessary for establishment of concentration equilibrium of the drugs investigated between erythrocytes and surrounding medium varies between a few seconds and several minutes, differences between the species being encountered. Hemoglobin binding of the drugs is much smaller than albumin binding. Nevertheless, drug concentration within the erythrocytes is generally higher than in the surrounding medium.", "PMID": 954483}
{"id": "pubmed23n0032_1168", "title": "Diagnostic evaluation of the juvenile offender: toward the clarification of often overlooked psychopathology.", "content": "On the basis of previously reported findings that a high percentage of court-referred children suffered from serious psychopathology other than sociopathy, attention is called to some essential, though often overlooked, elements of diagnostic evaluation of delinquents. Emphasis is on a meticulous history, including careful attention to the significances of family histories of serious psychiatric and/or neurological disorders. The importance of a detailed perinatal and medical history, with emphasis on determining a possible history of significant insult to the central nervous system, is stressed. A systematic mental status evaluation is strongly recommended. Techniques for assessing the existence of significant hallucinatory and delusional experiences in children are suggested. The uses of psychological and neurological evaluations to augment the psychiatric are discussed. The poor social situation from which many delinquents come as well as the obvious intrafamilial psychodynamic factors influencing delinquent behavior are recognized as frequent impediments to the search for less obvious psychotic and/or organic vulnerabilities in the juvenile offender. Case histories of several children who had previously been dismissed as merely sociopathic, illustrate the thesis of the paper.", "contents": "Diagnostic evaluation of the juvenile offender: toward the clarification of often overlooked psychopathology. On the basis of previously reported findings that a high percentage of court-referred children suffered from serious psychopathology other than sociopathy, attention is called to some essential, though often overlooked, elements of diagnostic evaluation of delinquents. Emphasis is on a meticulous history, including careful attention to the significances of family histories of serious psychiatric and/or neurological disorders. The importance of a detailed perinatal and medical history, with emphasis on determining a possible history of significant insult to the central nervous system, is stressed. A systematic mental status evaluation is strongly recommended. Techniques for assessing the existence of significant hallucinatory and delusional experiences in children are suggested. The uses of psychological and neurological evaluations to augment the psychiatric are discussed. The poor social situation from which many delinquents come as well as the obvious intrafamilial psychodynamic factors influencing delinquent behavior are recognized as frequent impediments to the search for less obvious psychotic and/or organic vulnerabilities in the juvenile offender. Case histories of several children who had previously been dismissed as merely sociopathic, illustrate the thesis of the paper.", "PMID": 954484}
{"id": "pubmed23n0032_1169", "title": "The psychological reactions of children with neurological problems.", "content": "The diagnosis of a neurological disorder can be confusing and frightening to a child, particularly if he is not given a reasonable explanation for his symptoms and the medical procedures that have to be carried out. If such children experience seizures, they frequently identify them with the possibility of death, and they become overly concerned about bodily functioning. A loss of self-esteem often results, particularly if there are school problems. Reactions are varied: some children become extremely cautious in their activities to avoid further seizures; some seem to \"defy death\" by placing themselves in dangerous situations; some misinterpret fairly benign events in terms of their misconceptions about themselves. Much distress can be avoided by providing adequate information both to the parents and the child and by giving them adequate opportunity to ask questions and ventilate their feelings, perhaps with a therapist, in this time of crisis.", "contents": "The psychological reactions of children with neurological problems. The diagnosis of a neurological disorder can be confusing and frightening to a child, particularly if he is not given a reasonable explanation for his symptoms and the medical procedures that have to be carried out. If such children experience seizures, they frequently identify them with the possibility of death, and they become overly concerned about bodily functioning. A loss of self-esteem often results, particularly if there are school problems. Reactions are varied: some children become extremely cautious in their activities to avoid further seizures; some seem to \"defy death\" by placing themselves in dangerous situations; some misinterpret fairly benign events in terms of their misconceptions about themselves. Much distress can be avoided by providing adequate information both to the parents and the child and by giving them adequate opportunity to ask questions and ventilate their feelings, perhaps with a therapist, in this time of crisis.", "PMID": 954485}
{"id": "pubmed23n0032_1170", "title": "The multiproblem family presents in a children's outpatient psychiatric clinic.", "content": "This study focuses on children from multiproblem families when they appear at an outpatient psychiatric clinic for children. The sample consisted of the first 50 children and their families referred to a large children's outpatient psychiatric facility early in 1974. A multiproblem family was defined in terms of the number of helping agencies and/or professionals contacted by family members within the 3-year period prior to the current psychiatric evaluation and by the number of households in which the designated child patient had lived. Eighteen percent of the 50 cases met the criteria established for a multiproblem family. These indicators successfully differentiated multiproblem from nonmultiproblem families along a number of socioeconomic, demographic, family health, and family stability dimensions. Special difficulties encountered in psychiatrically evaluating children of such multiproblem families are presented, and ways of dealing with them are discussed.", "contents": "The multiproblem family presents in a children's outpatient psychiatric clinic. This study focuses on children from multiproblem families when they appear at an outpatient psychiatric clinic for children. The sample consisted of the first 50 children and their families referred to a large children's outpatient psychiatric facility early in 1974. A multiproblem family was defined in terms of the number of helping agencies and/or professionals contacted by family members within the 3-year period prior to the current psychiatric evaluation and by the number of households in which the designated child patient had lived. Eighteen percent of the 50 cases met the criteria established for a multiproblem family. These indicators successfully differentiated multiproblem from nonmultiproblem families along a number of socioeconomic, demographic, family health, and family stability dimensions. Special difficulties encountered in psychiatrically evaluating children of such multiproblem families are presented, and ways of dealing with them are discussed.", "PMID": 954486}
{"id": "pubmed23n0032_1171", "title": "Parenting for foster parents.", "content": "A parenting course for foster parents was described with a follow-up evaluation utilized to determine its effectiveness. The content of this broad-based course included learning theory, principles and techniques, communication skills, methods of conflict resolution, developmental considerations, and information about community services. Results of the evaluation suggested that most of the participants had found the course helpful and were using the knowledge acquired.", "contents": "Parenting for foster parents. A parenting course for foster parents was described with a follow-up evaluation utilized to determine its effectiveness. The content of this broad-based course included learning theory, principles and techniques, communication skills, methods of conflict resolution, developmental considerations, and information about community services. Results of the evaluation suggested that most of the participants had found the course helpful and were using the knowledge acquired.", "PMID": 954487}
{"id": "pubmed23n0032_1172", "title": "Learning of imitate in infancy.", "content": "Theories of imitation learning are examined regarding their account of how the infant acquires the ability to emit a response which resembles a response previously exhibited by another. Each theory suffers from 1 or more of the following problems: logical inconsistency, incompleteness, and lack of empirical support. 3 of the views (associative thesis, discriminative learning thesis, and acquired value of the relational stimulus of similarity) are not mutually exclusive, and, therefore, they could be included in a theory of learning to imitate. However, none of these learning theses deals with the fact that imitation requires the infant to consistently abstract similar features from stimuli which differ on various dimensions. Hence, consideration is given to the role of cognition in a theory of learning to imitate.", "contents": "Learning of imitate in infancy. Theories of imitation learning are examined regarding their account of how the infant acquires the ability to emit a response which resembles a response previously exhibited by another. Each theory suffers from 1 or more of the following problems: logical inconsistency, incompleteness, and lack of empirical support. 3 of the views (associative thesis, discriminative learning thesis, and acquired value of the relational stimulus of similarity) are not mutually exclusive, and, therefore, they could be included in a theory of learning to imitate. However, none of these learning theses deals with the fact that imitation requires the infant to consistently abstract similar features from stimuli which differ on various dimensions. Hence, consideration is given to the role of cognition in a theory of learning to imitate.", "PMID": 954491}
{"id": "pubmed23n0032_1173", "title": "Rate of habituation and visual memory in infants.", "content": "This study investigated 17-week-old infants' response to discrepancy as a function of rate of habituation. 36 subjects were repeatedly shown a pattern containing 4 geometric shapes until they reached a proportional criterion of habituation: each infant's fixation time had to decrease by at least 50%. On the basis of 2 nonoverlapping distributions of trials to criterion, fast and slow habituators were identified. Recovery of fixation time was found for both fast and slow habituators when a discrepant stimulus was presented following habituation. These data were interpreted as reflecting developmental differences in encoding. After several interpolated trials with the new pattern, the infants' response to the original habituation stimulus increased, suggesting that the interpolated trials had interfered with their memory for the habituation stimulus.", "contents": "Rate of habituation and visual memory in infants. This study investigated 17-week-old infants' response to discrepancy as a function of rate of habituation. 36 subjects were repeatedly shown a pattern containing 4 geometric shapes until they reached a proportional criterion of habituation: each infant's fixation time had to decrease by at least 50%. On the basis of 2 nonoverlapping distributions of trials to criterion, fast and slow habituators were identified. Recovery of fixation time was found for both fast and slow habituators when a discrepant stimulus was presented following habituation. These data were interpreted as reflecting developmental differences in encoding. After several interpolated trials with the new pattern, the infants' response to the original habituation stimulus increased, suggesting that the interpolated trials had interfered with their memory for the habituation stimulus.", "PMID": 954492}
{"id": "pubmed23n0032_1174", "title": "Infant attention to auditory discrepancy.", "content": "Groups of 7 1/2-month-old infants heard 1 of 8 episodes consisting of no, slight, moderate, or a large discrepancy between a habituated standard and a transformed auditory stimulus. The patterns of cardiac deceleration, regarded as an index of attention supported the hypothesis that attentiveness is an inverted-U function of the degree of discrepancy between stimulus event and schema.", "contents": "Infant attention to auditory discrepancy. Groups of 7 1/2-month-old infants heard 1 of 8 episodes consisting of no, slight, moderate, or a large discrepancy between a habituated standard and a transformed auditory stimulus. The patterns of cardiac deceleration, regarded as an index of attention supported the hypothesis that attentiveness is an inverted-U function of the degree of discrepancy between stimulus event and schema.", "PMID": 954493}
{"id": "pubmed23n0032_1175", "title": "A preliminary study of some ecological correlates of child abuse: the impact of socioeconomic stress on mothers.", "content": "The ecological correlates of child abuse and maltreatment are explored. A model which focuses on the degree to which mothers are given support for the parent function is considered. This model, based on studies by Gil (1970) and Bronfenbrenner's (1974c) analysis of \"support systems for parents\", is examined empirically. Data on rates of child abuse/maltreatment for counties in New York State - based on reports made pursuant to a new, more stringent 1973 reporting law - are examined in light of indices of socioeconomic and demographic characteristics of the 58 counties under study. The data suggest that the degree to which mothers in a particular county are subjects to socioeconomic stress without adequate support systems accounts for a substantial proportion (36%) of the variance in rates of child abuse/maltreatment across New York counties, while economic conditions more generally affecting the family account for 16% of the variance. Application of them empirical model (based on the stepwise multiple regression analysis) to 2 additional samples of child abuse/maltreatment reports (1974) in New York State counties yields results consistent with the initial sample from which the multiple regression equation was drawn.", "contents": "A preliminary study of some ecological correlates of child abuse: the impact of socioeconomic stress on mothers. The ecological correlates of child abuse and maltreatment are explored. A model which focuses on the degree to which mothers are given support for the parent function is considered. This model, based on studies by Gil (1970) and Bronfenbrenner's (1974c) analysis of \"support systems for parents\", is examined empirically. Data on rates of child abuse/maltreatment for counties in New York State - based on reports made pursuant to a new, more stringent 1973 reporting law - are examined in light of indices of socioeconomic and demographic characteristics of the 58 counties under study. The data suggest that the degree to which mothers in a particular county are subjects to socioeconomic stress without adequate support systems accounts for a substantial proportion (36%) of the variance in rates of child abuse/maltreatment across New York counties, while economic conditions more generally affecting the family account for 16% of the variance. Application of them empirical model (based on the stepwise multiple regression analysis) to 2 additional samples of child abuse/maltreatment reports (1974) in New York State counties yields results consistent with the initial sample from which the multiple regression equation was drawn.", "PMID": 954494}
{"id": "pubmed23n0032_1176", "title": "Spectrum analysis of the cry sounds of well-nourished and malnourished infants.", "content": "The cry sounds of 12 well-nourished and 12 malnourished male infants were compared using behavioral and acoustic measures. The cry of the malnourished infant had an initial longer sound, higher pitch, lower amplitude, more arrhythmia, and a longer latency to the next cry sound than the cry of the well-nourished infant. The similarity betweenthe cry of the malnourished infant and the cry of the brain-damaged infant suggested tha malnutrition may affect the regulatory function of the central nervous system. This hypothesis was supported by additional findings which showed that the abnormal cry patterns in the malnourished infants were associated with a low-level orienting response to a pure tone stimulus as measured by the magnitude of heart-rate deceleration.", "contents": "Spectrum analysis of the cry sounds of well-nourished and malnourished infants. The cry sounds of 12 well-nourished and 12 malnourished male infants were compared using behavioral and acoustic measures. The cry of the malnourished infant had an initial longer sound, higher pitch, lower amplitude, more arrhythmia, and a longer latency to the next cry sound than the cry of the well-nourished infant. The similarity betweenthe cry of the malnourished infant and the cry of the brain-damaged infant suggested tha malnutrition may affect the regulatory function of the central nervous system. This hypothesis was supported by additional findings which showed that the abnormal cry patterns in the malnourished infants were associated with a low-level orienting response to a pure tone stimulus as measured by the magnitude of heart-rate deceleration.", "PMID": 954495}
{"id": "pubmed23n0032_1177", "title": "Short-term memory and equivalence judgments in normal and retarded readers.", "content": "Matched groups of normal and disabled readers were required to perform same/different judgments in which the interval between standard and comparison stimuli was either 0, 1, or 6 sec. Standard and comparison stimuli were either the same, different in element order, or different in actual elements present. Retarded readers performed as well as controls on same tasks in all 3 delay conditions. Difference tasks discriminated retarded readers on 1- and 6-sec conditions, but not on 0-sec conditions. Differences in order and gross element distinguished retarded from adequate readers equally well. The results are interpreted to indicate a deficit in short-term memory in retarded readers, but only for tasks requiring a difference judgment.", "contents": "Short-term memory and equivalence judgments in normal and retarded readers. Matched groups of normal and disabled readers were required to perform same/different judgments in which the interval between standard and comparison stimuli was either 0, 1, or 6 sec. Standard and comparison stimuli were either the same, different in element order, or different in actual elements present. Retarded readers performed as well as controls on same tasks in all 3 delay conditions. Difference tasks discriminated retarded readers on 1- and 6-sec conditions, but not on 0-sec conditions. Differences in order and gross element distinguished retarded from adequate readers equally well. The results are interpreted to indicate a deficit in short-term memory in retarded readers, but only for tasks requiring a difference judgment.", "PMID": 954496}
{"id": "pubmed23n0032_1178", "title": "Peripheral vision in children and adults.", "content": "Threshold luminance necessary to evoke an eye movement was evaluated as a function of age (6-21 years) and visual angle (6 degrees-42 degrees from the fovea). Results were comparable to those of static perimetry in older subjects but showed little narrowing of the visual field in younger children.", "contents": "Peripheral vision in children and adults. Threshold luminance necessary to evoke an eye movement was evaluated as a function of age (6-21 years) and visual angle (6 degrees-42 degrees from the fovea). Results were comparable to those of static perimetry in older subjects but showed little narrowing of the visual field in younger children.", "PMID": 954497}
{"id": "pubmed23n0032_1179", "title": "A critique of \"obstetrical pain-relieving drugs as predictors of infant behavior variability\".", "content": "Aleksandrowicz and Aleksandrowicz (1974) concluded that \"the use of obstetrical drugs is related to a considerable degree, to infantile behavior over the first month of life\" (p. 944). Questions are raised about the adequacy of a number of their procedures. These questions are serious enough to disturb the foundation for any conclusions.", "contents": "A critique of \"obstetrical pain-relieving drugs as predictors of infant behavior variability\". Aleksandrowicz and Aleksandrowicz (1974) concluded that \"the use of obstetrical drugs is related to a considerable degree, to infantile behavior over the first month of life\" (p. 944). Questions are raised about the adequacy of a number of their procedures. These questions are serious enough to disturb the foundation for any conclusions.", "PMID": 954498}
{"id": "pubmed23n0032_1180", "title": "The adjustment of reaching behavior to object distance in early infancy.", "content": "The prehensile activity and looking behavior of 2- and 5-month-old infants were videotaped in the presence of objects placed within and beyond possible contact distance. There was only a consistent adjustment of reaching behavior to stimulus distance in the case of the 5-month-old group. The distance discrimination evident in the prehensile activity of the older infants was paralleled by a reduction in the amount of attention paid to far objects.", "contents": "The adjustment of reaching behavior to object distance in early infancy. The prehensile activity and looking behavior of 2- and 5-month-old infants were videotaped in the presence of objects placed within and beyond possible contact distance. There was only a consistent adjustment of reaching behavior to stimulus distance in the case of the 5-month-old group. The distance discrimination evident in the prehensile activity of the older infants was paralleled by a reduction in the amount of attention paid to far objects.", "PMID": 954500}
{"id": "pubmed23n0032_1181", "title": "The effects of training specific mnemonics on the metamnemonic efficiency of retarded children.", "content": "The effects of training specific mnemonic skills on recall readiness were evaluated in 2 groups (MA 6, MA 8) of educable retarded children. Equal numbers of S at each age were instructed in an anticipation, rehearsal, or label strategy. On the first posttest (prompted), label training did not affect performance, but anticipation and rehearsal training facilitated recall readiness estimation for both younger and older children. However, on 2 subsequent posttests (unprompted, 2 days and 2 weeks after training), the younger S returned to their pretraining competency, while the older group maintained high performance. Consideration of observational data suggested that overt activity related to the trained mnemonic correlated with recall readiness efficiency for the older but not the younger group. Even though younger children produced the strategy, the failed to monitor its effectiveness. The implications of training specific mnemonic skills are discussed in relation to developing metamnemonic efficiency.", "contents": "The effects of training specific mnemonics on the metamnemonic efficiency of retarded children. The effects of training specific mnemonic skills on recall readiness were evaluated in 2 groups (MA 6, MA 8) of educable retarded children. Equal numbers of S at each age were instructed in an anticipation, rehearsal, or label strategy. On the first posttest (prompted), label training did not affect performance, but anticipation and rehearsal training facilitated recall readiness estimation for both younger and older children. However, on 2 subsequent posttests (unprompted, 2 days and 2 weeks after training), the younger S returned to their pretraining competency, while the older group maintained high performance. Consideration of observational data suggested that overt activity related to the trained mnemonic correlated with recall readiness efficiency for the older but not the younger group. Even though younger children produced the strategy, the failed to monitor its effectiveness. The implications of training specific mnemonic skills are discussed in relation to developing metamnemonic efficiency.", "PMID": 954501}
{"id": "pubmed23n0032_1182", "title": "Children's recognition memory for written sentences: a comparison of good poor readers.", "content": "In 18 pairs of fifth-grade males matched on sex, CA, and IQ, 1 was a good reader and 1 a poor reader. Each child read 8 3-sentence acquisition stories, subsequently performed a card-sorting task, and finally a recognition test consisting of 40 sentences which either were in the acquisition set or were 1 of 5 kinds of transformation. Retention was better for semantic aspects of the stories, but retention of tense was poor. Good readers mad fewer recognition errors than poor readers on new sentences which were true inferences from the acquisition list and on tests for retention of tense and number.", "contents": "Children's recognition memory for written sentences: a comparison of good poor readers. In 18 pairs of fifth-grade males matched on sex, CA, and IQ, 1 was a good reader and 1 a poor reader. Each child read 8 3-sentence acquisition stories, subsequently performed a card-sorting task, and finally a recognition test consisting of 40 sentences which either were in the acquisition set or were 1 of 5 kinds of transformation. Retention was better for semantic aspects of the stories, but retention of tense was poor. Good readers mad fewer recognition errors than poor readers on new sentences which were true inferences from the acquisition list and on tests for retention of tense and number.", "PMID": 954502}
{"id": "pubmed23n0032_1183", "title": "[Modification of the oxygen supply of the small intestine demonstrated on the ileus model].", "content": "Elevation of the intraluminar pressure of rabbit ileum lowers the oxygen supply of the bowel wall in a pressure dependent manner. After decompression local PO2 exceeds the initial values. The infusion of Rheomacrodex-Sorbit induces an increase of local PO2 in the nondistended ileum. The reaction is enhanced in the distended bowel. The application of glucagon does not influence tissue PO2 significantly in either the distended or the undistended bowel. The infusion of Rheomacrodex-Sorbit as well as decompression of the distended bowel are recommended to improve oxygen supply of bowel wall in ileus.", "contents": "[Modification of the oxygen supply of the small intestine demonstrated on the ileus model]. Elevation of the intraluminar pressure of rabbit ileum lowers the oxygen supply of the bowel wall in a pressure dependent manner. After decompression local PO2 exceeds the initial values. The infusion of Rheomacrodex-Sorbit induces an increase of local PO2 in the nondistended ileum. The reaction is enhanced in the distended bowel. The application of glucagon does not influence tissue PO2 significantly in either the distended or the undistended bowel. The infusion of Rheomacrodex-Sorbit as well as decompression of the distended bowel are recommended to improve oxygen supply of bowel wall in ileus.", "PMID": 954506}
{"id": "pubmed23n0032_1184", "title": "[Treatment of pathologic fractures].", "content": "Eight pathologic fractures of benign and 52 of malignant origin are reported. Open reduction and rigid internal fixation should be performed to give the patient the use of his affected limb as soon as this can be accomplished. Treatment consists of internal fixation in lesions of the shaft and prosthetic replacement in the case of lesions near joints. Only very rarely a primary amputation must be performed. Malignant fractures are most frequently caused by metastases from breast cancers. The treatment will at least make nursing easier, and three-quarters of these patients can be mobilized. The combination of surgical treatment and radiotherapy is discussed. In spite of this approach the average time of survival of 14 months is short because a pathological fracture due to a malignant tumor is a late symptom of the disease.", "contents": "[Treatment of pathologic fractures]. Eight pathologic fractures of benign and 52 of malignant origin are reported. Open reduction and rigid internal fixation should be performed to give the patient the use of his affected limb as soon as this can be accomplished. Treatment consists of internal fixation in lesions of the shaft and prosthetic replacement in the case of lesions near joints. Only very rarely a primary amputation must be performed. Malignant fractures are most frequently caused by metastases from breast cancers. The treatment will at least make nursing easier, and three-quarters of these patients can be mobilized. The combination of surgical treatment and radiotherapy is discussed. In spite of this approach the average time of survival of 14 months is short because a pathological fracture due to a malignant tumor is a late symptom of the disease.", "PMID": 954507}
{"id": "pubmed23n0032_1185", "title": "[Agastric dystrophy after gastrectomy].", "content": "A follow-up of 9 patients was performed 5-11 years subsequent to total gastrectomy. Biochemical findings were normal. Compared to a normal control group vitamin A absorption indicative of fat assimilation was unaltered. Subjects regained preoperative weight and presented no signs of malnutrition. Eight patients suffered seriously from reflux. Five patients showed dumping symptoms. Endoscopic specimens from jejunal mucosa were found to be normal. It seems that postoperative complaints described above may be prevented by reasonable operative measures.", "contents": "[Agastric dystrophy after gastrectomy]. A follow-up of 9 patients was performed 5-11 years subsequent to total gastrectomy. Biochemical findings were normal. Compared to a normal control group vitamin A absorption indicative of fat assimilation was unaltered. Subjects regained preoperative weight and presented no signs of malnutrition. Eight patients suffered seriously from reflux. Five patients showed dumping symptoms. Endoscopic specimens from jejunal mucosa were found to be normal. It seems that postoperative complaints described above may be prevented by reasonable operative measures.", "PMID": 954513}
{"id": "pubmed23n0032_1186", "title": "[Primary retroperitoneal tumors. Symptoms-diagnosis--therapy].", "content": "Symptoms, diagnosis, and therapy of primary retroperitoneal tumors are presented with reference to 50 of the authors' cases. Seventy to 80% of the tumors are malignant; the most frequent are sarcomas. Males and females are equally afflicted; most cases are found within the 50-60 yearage range. First symptom is generally the palpable abdominal tumor. The diagnostic procedure of choic ist i.v. urography as well as selective angiograpms. At the time of diagnosis only 30-40% of tumors can be resected. Postoperative mortality ist about 10%. Seventy percent of tumors are radiosensitive. Prognosis can be considerably improved by postoperative radiation. Survival times of more than 2 years in patients presenting with malignant primary retroperitoneal tumors are exceptional.", "contents": "[Primary retroperitoneal tumors. Symptoms-diagnosis--therapy]. Symptoms, diagnosis, and therapy of primary retroperitoneal tumors are presented with reference to 50 of the authors' cases. Seventy to 80% of the tumors are malignant; the most frequent are sarcomas. Males and females are equally afflicted; most cases are found within the 50-60 yearage range. First symptom is generally the palpable abdominal tumor. The diagnostic procedure of choic ist i.v. urography as well as selective angiograpms. At the time of diagnosis only 30-40% of tumors can be resected. Postoperative mortality ist about 10%. Seventy percent of tumors are radiosensitive. Prognosis can be considerably improved by postoperative radiation. Survival times of more than 2 years in patients presenting with malignant primary retroperitoneal tumors are exceptional.", "PMID": 954514}
{"id": "pubmed23n0032_1187", "title": "[Cartilaginous tumors of the skeleton].", "content": "Chondrogenic tumors of the skeleton are divided into 6 separate groups: 1. chondroblastomas 2. chondromyxoidfibromas, 3. osteochondromas (cartilaginous exostoses), 4. enchondromas, 5. periostal chondromas, and 6. chondrosarcomas. The presentation of the different types of tumors demonstrates the pathogenesis, pathology, clinical findings, treatment, and prognosis. States of tumors before and after operative treatment are illustrated.", "contents": "[Cartilaginous tumors of the skeleton]. Chondrogenic tumors of the skeleton are divided into 6 separate groups: 1. chondroblastomas 2. chondromyxoidfibromas, 3. osteochondromas (cartilaginous exostoses), 4. enchondromas, 5. periostal chondromas, and 6. chondrosarcomas. The presentation of the different types of tumors demonstrates the pathogenesis, pathology, clinical findings, treatment, and prognosis. States of tumors before and after operative treatment are illustrated.", "PMID": 954515}
{"id": "pubmed23n0032_1188", "title": "[Cultivation of the epithelium in burns].", "content": "Comparison of two groups of burned patients shows that patients who were treated by wound solution stayed a significantly shorter time in hospital. The group with healing under the scab (PQB) had indeed slighter skin lesions, but syated a much longer time in hospital. The great advantage of treatment of burned wounds with wound solution is seen in the relief of pain of the patients. Aslo, physical exercises can begin immediately. Wound solution needs no anaesthesia for necrolysis. The shortening of the healing time results in stimulation of the marginal epithelium and growth of autologous transplanted cells on the wound.", "contents": "[Cultivation of the epithelium in burns]. Comparison of two groups of burned patients shows that patients who were treated by wound solution stayed a significantly shorter time in hospital. The group with healing under the scab (PQB) had indeed slighter skin lesions, but syated a much longer time in hospital. The great advantage of treatment of burned wounds with wound solution is seen in the relief of pain of the patients. Aslo, physical exercises can begin immediately. Wound solution needs no anaesthesia for necrolysis. The shortening of the healing time results in stimulation of the marginal epithelium and growth of autologous transplanted cells on the wound.", "PMID": 954516}
{"id": "pubmed23n0032_1189", "title": "[Polyposis coli. Comparison between the familial and non-familial form].", "content": "Since 1970 16 patients with polyposis coli have been treated at the Department of Surgery, Medical School, Hannover. Of these, 9 patients showed the classic type of familial polyposis, while 7 patients denied a positive family history. Comparing both groups, the nonfamilial type of polyposis coli can be separated as well from the familial polyposis as from multiple adenomas of the large bowel. Operative treatment in both groups of polyposis coli and management of nonfamilial polyposis coli are discussed.", "contents": "[Polyposis coli. Comparison between the familial and non-familial form]. Since 1970 16 patients with polyposis coli have been treated at the Department of Surgery, Medical School, Hannover. Of these, 9 patients showed the classic type of familial polyposis, while 7 patients denied a positive family history. Comparing both groups, the nonfamilial type of polyposis coli can be separated as well from the familial polyposis as from multiple adenomas of the large bowel. Operative treatment in both groups of polyposis coli and management of nonfamilial polyposis coli are discussed.", "PMID": 954522}
{"id": "pubmed23n0032_1190", "title": "[Preserving of continence in the treatment of familial polyposis of the colon. Technic of peeling off of the rectal mucosa].", "content": "The paper deals with the problem of preserving the rectum in the therapy of familial polyposis of the colon. According to our own experience and recent publications we cannot support the preservations of the rectum. Nevertheless, in order to preserve continence, we peel off the cylinder of the mucosa of the rest of the rectum (Devine und Ravitch). We have modified this procedure according to the Hochenegg pull-through and have operated successfully on two patients.", "contents": "[Preserving of continence in the treatment of familial polyposis of the colon. Technic of peeling off of the rectal mucosa]. The paper deals with the problem of preserving the rectum in the therapy of familial polyposis of the colon. According to our own experience and recent publications we cannot support the preservations of the rectum. Nevertheless, in order to preserve continence, we peel off the cylinder of the mucosa of the rest of the rectum (Devine und Ravitch). We have modified this procedure according to the Hochenegg pull-through and have operated successfully on two patients.", "PMID": 954523}
{"id": "pubmed23n0032_1191", "title": "[Preliminary observations on the clinical use of calcitonin. Treatment and postoperative prevention of pancreatitis].", "content": "Calcitonin was administered to 4 patients presenting with acute pancreatitis as well as to 6 patients presenting with postoperative pancreatitis. Prophylactic administration was performed in 3 cases consecutive to pancreatic interventions. Following calcitonin all patients showed considerable improvement of clinical course. Although 8 patients recuperated completely 2 patients died from independent complications. After prophylactic administration of calcitonin the postoperative course was uncomplicated without demonstrable increase in serum amylase. It is emphasized that only early case histories are reported; their verification will depend upon the results of a controlled survey presently being performed.", "contents": "[Preliminary observations on the clinical use of calcitonin. Treatment and postoperative prevention of pancreatitis]. Calcitonin was administered to 4 patients presenting with acute pancreatitis as well as to 6 patients presenting with postoperative pancreatitis. Prophylactic administration was performed in 3 cases consecutive to pancreatic interventions. Following calcitonin all patients showed considerable improvement of clinical course. Although 8 patients recuperated completely 2 patients died from independent complications. After prophylactic administration of calcitonin the postoperative course was uncomplicated without demonstrable increase in serum amylase. It is emphasized that only early case histories are reported; their verification will depend upon the results of a controlled survey presently being performed.", "PMID": 954524}
{"id": "pubmed23n0032_1192", "title": "[Histological research on the effect of viruses on wound healing].", "content": "The influence of pure virus solution on wound healing was studied in animals. The rate of disturbed wound healing was proportional to the concentration of the virus solution. Severe necrosis without inflammatory reaction could be demonstrated histologically.", "contents": "[Histological research on the effect of viruses on wound healing]. The influence of pure virus solution on wound healing was studied in animals. The rate of disturbed wound healing was proportional to the concentration of the virus solution. Severe necrosis without inflammatory reaction could be demonstrated histologically.", "PMID": 954525}
{"id": "pubmed23n0032_1193", "title": "[Treatment and prognosis in vertebral fractures in children and adolescents].", "content": "Twenty-six children and adolescents with a total of 65 vertebral fractures are reported. Sixteen patients could be followed up an average of 3 1/4 years after the accident. The questions of growth disturbance after vertebral body fractures and adequate treatment are chiefly considered. -Most of the fractures occurred in the midthoracic spine. As a rule they were serial fractures. -Fractures of the vertebral body with sagittal wedge deformity alone have a better prognosis than those with concomitant sagittal and frontal wedge deformities. Whereas those in the first group correct themselves partially or completely during subsequent growth, improvement in the wedge deformity was only observed in about one-third of the patients in the second group. When the end-plates were fractured, there was no correction and there was a lack in vertebral growth. Severe destruction of the cartilagenous plates and intervertebral discs led to a fusion of the corresponding segment. Increase in wedge deformity was observed twice. -Slight axial deviations of the intervertebral discs following vertebral body fractures are compensated for during growth in most cases. In comminuted fractures, the axial deviation persists but can be compensated for by the adjacent segments of the spine. In instable fractures, it can increase in spite of treatment in a Milwaukee corset. -No difference was found between the children treated with a hyperextension plaster corset and those treated functionally. On the basis of the results, the fracture types which should be treated functionally and those for which a plaster cast is recommended are indicated.", "contents": "[Treatment and prognosis in vertebral fractures in children and adolescents]. Twenty-six children and adolescents with a total of 65 vertebral fractures are reported. Sixteen patients could be followed up an average of 3 1/4 years after the accident. The questions of growth disturbance after vertebral body fractures and adequate treatment are chiefly considered. -Most of the fractures occurred in the midthoracic spine. As a rule they were serial fractures. -Fractures of the vertebral body with sagittal wedge deformity alone have a better prognosis than those with concomitant sagittal and frontal wedge deformities. Whereas those in the first group correct themselves partially or completely during subsequent growth, improvement in the wedge deformity was only observed in about one-third of the patients in the second group. When the end-plates were fractured, there was no correction and there was a lack in vertebral growth. Severe destruction of the cartilagenous plates and intervertebral discs led to a fusion of the corresponding segment. Increase in wedge deformity was observed twice. -Slight axial deviations of the intervertebral discs following vertebral body fractures are compensated for during growth in most cases. In comminuted fractures, the axial deviation persists but can be compensated for by the adjacent segments of the spine. In instable fractures, it can increase in spite of treatment in a Milwaukee corset. -No difference was found between the children treated with a hyperextension plaster corset and those treated functionally. On the basis of the results, the fracture types which should be treated functionally and those for which a plaster cast is recommended are indicated.", "PMID": 954526}
{"id": "pubmed23n0032_1194", "title": "Localization by Q-banding of mitotic chiasmata in cases of Bloom's syndrome.", "content": "The distribution of mitotic chiasmata from the lymphocytes of three patients with Bloom's syndrome was studied by Q-banding. An estimate was made of the average brightness of the human chromosomes and of the brightness and length of the Q-regions. A strong relationship was found between the average darkness of a chromosome and its chiasma density. The shorter and darker Q-regions contained more chiasmata, with the exception of the chromosome tips which had lower chiasma densities. A few \"hot spots\" had particularly high chiasma densities. Centric regions contained 17% of the chiasmata. The data are consistent with the assumptions that chiasmata are favored in darker regions and at borders between light and dark regions.", "contents": "Localization by Q-banding of mitotic chiasmata in cases of Bloom's syndrome. The distribution of mitotic chiasmata from the lymphocytes of three patients with Bloom's syndrome was studied by Q-banding. An estimate was made of the average brightness of the human chromosomes and of the brightness and length of the Q-regions. A strong relationship was found between the average darkness of a chromosome and its chiasma density. The shorter and darker Q-regions contained more chiasmata, with the exception of the chromosome tips which had lower chiasma densities. A few \"hot spots\" had particularly high chiasma densities. Centric regions contained 17% of the chiasmata. The data are consistent with the assumptions that chiasmata are favored in darker regions and at borders between light and dark regions.", "PMID": 954549}
{"id": "pubmed23n0032_1195", "title": "BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes.", "content": "BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolution method for determining the interchange points of X-X and X-autosome translocations. Asynchrony among terminal replication patterns of multiple late replicating X chromosomes within an individual cell can occasionally be demonstrated. The arms of isochromosomes usually exhibit symmetrical fluorescence patterns, with replication terminating in bands Xq21 and Xq23 (predominant pattern) or in bands Xq25 and Xq27 (alternative pattern) in both arms. In the vast majority of lymphocytes containing a balanced X-13 or X-19 translocation, the normal X is late replicating. However, DNA synthesis in the translocation products occasionally appears somewhat delayed relative to that expected for an early replicating X, consistent with possible position effects on replication kinetics.", "contents": "BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes. BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolution method for determining the interchange points of X-X and X-autosome translocations. Asynchrony among terminal replication patterns of multiple late replicating X chromosomes within an individual cell can occasionally be demonstrated. The arms of isochromosomes usually exhibit symmetrical fluorescence patterns, with replication terminating in bands Xq21 and Xq23 (predominant pattern) or in bands Xq25 and Xq27 (alternative pattern) in both arms. In the vast majority of lymphocytes containing a balanced X-13 or X-19 translocation, the normal X is late replicating. However, DNA synthesis in the translocation products occasionally appears somewhat delayed relative to that expected for an early replicating X, consistent with possible position effects on replication kinetics.", "PMID": 954550}
{"id": "pubmed23n0032_1196", "title": "Relative DNA contents of somatic nuclei of ox, sheep and goat.", "content": "Diploid ox nuclei contain about 14% more DNA than nuclei from sheep of the same sex. Goat nuclei have a similar DNA content to those of sheep. In view of the similar chromosome banding patterns in these species, it appears that chromosome evolution must have involved numerous minute interstitial deletions of additions of DNA. Although chromosomes which have similar banding patterns in these three species may be regarded as homologous in this respect, and can be regarded as having a common evolutionary origin, they are not homologous for the quantity of their DNA.", "contents": "Relative DNA contents of somatic nuclei of ox, sheep and goat. Diploid ox nuclei contain about 14% more DNA than nuclei from sheep of the same sex. Goat nuclei have a similar DNA content to those of sheep. In view of the similar chromosome banding patterns in these species, it appears that chromosome evolution must have involved numerous minute interstitial deletions of additions of DNA. Although chromosomes which have similar banding patterns in these three species may be regarded as homologous in this respect, and can be regarded as having a common evolutionary origin, they are not homologous for the quantity of their DNA.", "PMID": 954551}
{"id": "pubmed23n0032_1197", "title": "The chromosomal localisation of satellite DNA in Ptyas mucosus (Ophidia, Colubridae).", "content": "Ptyas mucosus male DNA has a repetitious DNA satellite (p = 1.700 g cm-3) constituting 5% of the haploid genome. In situ hybridisation of radioactive complementary RNA (cRNA) has revealed that satellite sequences are located in the centromeric region of one pair of macrochromosomes and in the terminal region of 8 pairs of microchromosomes. These regions are constitutively heterochromatic as revealed by C-banding. The possibility of involvement of satellite rich microchromosomes in nucleolus organisation is discussed.", "contents": "The chromosomal localisation of satellite DNA in Ptyas mucosus (Ophidia, Colubridae). Ptyas mucosus male DNA has a repetitious DNA satellite (p = 1.700 g cm-3) constituting 5% of the haploid genome. In situ hybridisation of radioactive complementary RNA (cRNA) has revealed that satellite sequences are located in the centromeric region of one pair of macrochromosomes and in the terminal region of 8 pairs of microchromosomes. These regions are constitutively heterochromatic as revealed by C-banding. The possibility of involvement of satellite rich microchromosomes in nucleolus organisation is discussed.", "PMID": 954552}
{"id": "pubmed23n0032_1198", "title": "Proportionate representation of rDNA and Balbiani ring DNA in polytene chromosomes of Chironomus tentans.", "content": "Attention was focussed on the question whether the most active genes of the polytene genome of Chironomus tentans, i.e. rDNA and Balbiani rings, have undergone the same number of duplications as the bulk of the genome. RNA was extracted from isolated salivary glands, enriched for messenger RNA by poly U sepharose chromatography, and labelled with 125Iodine. In situ hybridization showed that, apart from rRNA, Balbiani ring 1 and 2 transcripts are the major hybridizing RNA species present in this enriched preparation. BR1 hybridized only at the b-region. Transcripts of BR6 and puff I 17B are shown to be salivary gland specific and hybridize in situ to an extent of less than 5% of the RNA hybridized to BR1 plus BR2. Saturation hybridization levels of rRNA and Balbiani ring (BR1 plus BR2) RNA with polytene DNA from salivary glands were determined and compared with the saturation levels measured for DNA from adult flies and larvae. rRNA hybridized to the same level (0.09%) with polytene DNA as with DNA from primarily diploid cells (adult fly DNA) or from larvae. An equal hybridization level (0.04%) with each of these three types of DNA was also obtained for BR RNA. The results demonstrate that, during polytenization in C. tentans, the genes for rRNA and BR1 and 2 are duplicated to the same extent as the bulk of the genome.", "contents": "Proportionate representation of rDNA and Balbiani ring DNA in polytene chromosomes of Chironomus tentans. Attention was focussed on the question whether the most active genes of the polytene genome of Chironomus tentans, i.e. rDNA and Balbiani rings, have undergone the same number of duplications as the bulk of the genome. RNA was extracted from isolated salivary glands, enriched for messenger RNA by poly U sepharose chromatography, and labelled with 125Iodine. In situ hybridization showed that, apart from rRNA, Balbiani ring 1 and 2 transcripts are the major hybridizing RNA species present in this enriched preparation. BR1 hybridized only at the b-region. Transcripts of BR6 and puff I 17B are shown to be salivary gland specific and hybridize in situ to an extent of less than 5% of the RNA hybridized to BR1 plus BR2. Saturation hybridization levels of rRNA and Balbiani ring (BR1 plus BR2) RNA with polytene DNA from salivary glands were determined and compared with the saturation levels measured for DNA from adult flies and larvae. rRNA hybridized to the same level (0.09%) with polytene DNA as with DNA from primarily diploid cells (adult fly DNA) or from larvae. An equal hybridization level (0.04%) with each of these three types of DNA was also obtained for BR RNA. The results demonstrate that, during polytenization in C. tentans, the genes for rRNA and BR1 and 2 are duplicated to the same extent as the bulk of the genome.", "PMID": 954553}
{"id": "pubmed23n0032_1199", "title": "RNA, RIBOSOMAL/*BIOSYNon of ribosomal 5S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA.", "content": "5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 10(7) dpm/mug with carrier-free iodine-125. After hybridization in situ of 125I-5 S RNA and autoradiography only region B3c-e (containing two \"normal\" and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly \"heterozygous\" labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorportion in spite of a continuous synthesis of 5 S RNA in salivary gland cells.", "contents": "RNA, RIBOSOMAL/*BIOSYNon of ribosomal 5S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA. 5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 10(7) dpm/mug with carrier-free iodine-125. After hybridization in situ of 125I-5 S RNA and autoradiography only region B3c-e (containing two \"normal\" and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly \"heterozygous\" labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorportion in spite of a continuous synthesis of 5 S RNA in salivary gland cells.", "PMID": 954554}
{"id": "pubmed23n0032_1200", "title": "Psychotic exacerbations produced by neuroleptics.", "content": "Thirteen schizophrenic patients who developed abnormal psychotic behavior as an adverse reaction to a neuroleptic are described. A. Three patients showed a marked increase in the psychopathology during neuroleptic treatment. These episodes were treated by decreasing or discontinuing the neuroleptics. They did not respond to anticholinergic durgs nor did they respond to an increase in dosage, (another side effect previously reported and referred to here) indeed this treatment worsened the situation. B. Ten patients showed a mixed picture of catatonic excitement or inhibition on neuroleptics and several developed hallucinatory episodes. All of these exacerbations were terminated by anticholinergic injections. Other more familiar CNS abnormalities produced by neuroleptics are briefly discussed.", "contents": "Psychotic exacerbations produced by neuroleptics. Thirteen schizophrenic patients who developed abnormal psychotic behavior as an adverse reaction to a neuroleptic are described. A. Three patients showed a marked increase in the psychopathology during neuroleptic treatment. These episodes were treated by decreasing or discontinuing the neuroleptics. They did not respond to anticholinergic durgs nor did they respond to an increase in dosage, (another side effect previously reported and referred to here) indeed this treatment worsened the situation. B. Ten patients showed a mixed picture of catatonic excitement or inhibition on neuroleptics and several developed hallucinatory episodes. All of these exacerbations were terminated by anticholinergic injections. Other more familiar CNS abnormalities produced by neuroleptics are briefly discussed.", "PMID": 954560}
{"id": "pubmed23n0032_1201", "title": "Psychotropic drugs in opioid addicts on methadone treatment.", "content": "Psychotropic drug treatment of persons on methadone maintenance is discussed. Patients with clear target symptoms, such as anxiety, depression, or psychosis responded just as non-opioid addicts would to the major psychotropic agents. The minor tranquilizers are felt to be of doubtful value, and subject to abuse. Sleep disturbances cannot be treated by the usual means, as the drugs needed again are abused. However, chlorpromazine shows some promise here. Methods of drug delivery and goals of treatment must be adapted to the realities of this patient-group's characteristics, particularly anti-social traits, poor motivation and unreliability. Psychotropic drugs are unlikely to be of aid in multiple drug abusers, personality and character disorders, and opioid withdrawal. Four case histories are presented.", "contents": "Psychotropic drugs in opioid addicts on methadone treatment. Psychotropic drug treatment of persons on methadone maintenance is discussed. Patients with clear target symptoms, such as anxiety, depression, or psychosis responded just as non-opioid addicts would to the major psychotropic agents. The minor tranquilizers are felt to be of doubtful value, and subject to abuse. Sleep disturbances cannot be treated by the usual means, as the drugs needed again are abused. However, chlorpromazine shows some promise here. Methods of drug delivery and goals of treatment must be adapted to the realities of this patient-group's characteristics, particularly anti-social traits, poor motivation and unreliability. Psychotropic drugs are unlikely to be of aid in multiple drug abusers, personality and character disorders, and opioid withdrawal. Four case histories are presented.", "PMID": 954561}
{"id": "pubmed23n0032_1202", "title": "Depressive syndrome induced by oral contraceptives.", "content": "It has been demonstrated that a small proportion of women taking oral contraceptives develop a depressive syndrome characterized by despondency, tension, and changes in sex desire. Although disturbed tryptophan metabolism induced by estrogen has been held responsible for this syndrome, the role of psychological factors should not be overlooked. Recognition and management of the syndrome require information concerning the pharmacological actions of oral contraceptives and the personality make-up of the patient.", "contents": "Depressive syndrome induced by oral contraceptives. It has been demonstrated that a small proportion of women taking oral contraceptives develop a depressive syndrome characterized by despondency, tension, and changes in sex desire. Although disturbed tryptophan metabolism induced by estrogen has been held responsible for this syndrome, the role of psychological factors should not be overlooked. Recognition and management of the syndrome require information concerning the pharmacological actions of oral contraceptives and the personality make-up of the patient.", "PMID": 954562}
{"id": "pubmed23n0032_1203", "title": "A brief method of screening for alcoholism.", "content": "Subjects given a research diagnosis of alcoholism were evaluated to determine whether key symptoms exist which provide a means of rapid screening for alcoholism. Alcoholic subjects were compared with a large control group of non-alcoholics. A very high percent of alcoholics was identified by the subject's report that he or others believed the subject drank too much. Few non-alcoholic subjects reported that either they or others had such beliefs. Thus, several easy questions can serve as highly reliable screening tests for alcoholism.", "contents": "A brief method of screening for alcoholism. Subjects given a research diagnosis of alcoholism were evaluated to determine whether key symptoms exist which provide a means of rapid screening for alcoholism. Alcoholic subjects were compared with a large control group of non-alcoholics. A very high percent of alcoholics was identified by the subject's report that he or others believed the subject drank too much. Few non-alcoholic subjects reported that either they or others had such beliefs. Thus, several easy questions can serve as highly reliable screening tests for alcoholism.", "PMID": 954564}
{"id": "pubmed23n0032_1204", "title": "Medicinal use of hypodermic needles in an addict patient population.", "content": "Narcotic addicts may become so conditioned to the use of hypodermic needles that self-injection without a drug may become pleasurable. This phenomenon has raised the question as to whether addicts in treatment should be administered any medication by hypodermic needle for fear of precipitating withdrawal or inducing a craving for heroin. In this study addicts in treatment were given a medicinal needle challenge but there were no documented deleterious effects.", "contents": "Medicinal use of hypodermic needles in an addict patient population. Narcotic addicts may become so conditioned to the use of hypodermic needles that self-injection without a drug may become pleasurable. This phenomenon has raised the question as to whether addicts in treatment should be administered any medication by hypodermic needle for fear of precipitating withdrawal or inducing a craving for heroin. In this study addicts in treatment were given a medicinal needle challenge but there were no documented deleterious effects.", "PMID": 954565}
{"id": "pubmed23n0032_1205", "title": "Treatment of a 15 year old hebephrenic girl in a community hospital.", "content": "The preceeding discussion and case histroy illustrate the modern eclecticism which must be utilized in a synergistic manner to effect remissions in psychiatric illnesses heretofore unresponsive to treatment. The community psychiatrist must be capable of functioning in the face of adversity and sometimes must receive the criticism of his peers with equanimity and tolerance by accepting the opportunity as a challenge to educate colleagues to the full potentiality of modern psychiatry.", "contents": "Treatment of a 15 year old hebephrenic girl in a community hospital. The preceeding discussion and case histroy illustrate the modern eclecticism which must be utilized in a synergistic manner to effect remissions in psychiatric illnesses heretofore unresponsive to treatment. The community psychiatrist must be capable of functioning in the face of adversity and sometimes must receive the criticism of his peers with equanimity and tolerance by accepting the opportunity as a challenge to educate colleagues to the full potentiality of modern psychiatry.", "PMID": 954567}
{"id": "pubmed23n0032_1206", "title": "[Poliomyelitis epidemic in a district of Freiburg i. Br. in autumn 1975 (author's transl)].", "content": "In the autumn of 1975 five cases of poliomyelitis occurred within 6 weeks in German children of families of low socioeconomic class. They lived in two districts of Freiburg with close familial and occupational contacts. One child had been immunised against polio once orally several years ago and none of the others were immunised. The clinical course and results of investigations of the environment are reported. Included is the state of immunisation of 472 school beginners in Freiburg schools and the antibody levels of 284 children aged 1 to 10 years from Freiburg and the adjoining areas.", "contents": "[Poliomyelitis epidemic in a district of Freiburg i. Br. in autumn 1975 (author's transl)]. In the autumn of 1975 five cases of poliomyelitis occurred within 6 weeks in German children of families of low socioeconomic class. They lived in two districts of Freiburg with close familial and occupational contacts. One child had been immunised against polio once orally several years ago and none of the others were immunised. The clinical course and results of investigations of the environment are reported. Included is the state of immunisation of 472 school beginners in Freiburg schools and the antibody levels of 284 children aged 1 to 10 years from Freiburg and the adjoining areas.", "PMID": 954590}
{"id": "pubmed23n0032_1207", "title": "[Endocarditis lenta caused by Lactobacillus salivarius subsp. salicinicus (author's transl)].", "content": "After two tooth extractions performed without antibiotic cover endocarditis lenta occurred in a ten-year-old girl. The causative organism isolated was Lactobacillus salivarius subsp. salicinicus, the first such reported case. The child has a small, haemodynamically insignificant, ventricular septal defect. A cure was achieved after long-term administration of penicillin G in high doses, at first combined with ampicillin. There were no complications.", "contents": "[Endocarditis lenta caused by Lactobacillus salivarius subsp. salicinicus (author's transl)]. After two tooth extractions performed without antibiotic cover endocarditis lenta occurred in a ten-year-old girl. The causative organism isolated was Lactobacillus salivarius subsp. salicinicus, the first such reported case. The child has a small, haemodynamically insignificant, ventricular septal defect. A cure was achieved after long-term administration of penicillin G in high doses, at first combined with ampicillin. There were no complications.", "PMID": 954591}
{"id": "pubmed23n0032_1208", "title": "[The influence of saralasine on blood pressure and renal function in Bartter's syndrome and decompensated hepatic cirrhosis (author's transl)].", "content": "Infusion of the angiotensin-II-antagonist saralasine led in one patient with Bartter's syndrome and one patient with decompensated hepatic cirrhosis, both of whom had a markedly raised plasma renin activity, to a fall in systolic and diastolic blood pressure. The results indicate that in normotensive patients a raised angiotensin II concentration in blood is haemodynamically important for the level of blood pressure if plasma renin activity is raised. In normotensives with normal plasma renin activity saralasine in the usual dosage (4.2 mug/kg-min) does not influence the blood pressure.", "contents": "[The influence of saralasine on blood pressure and renal function in Bartter's syndrome and decompensated hepatic cirrhosis (author's transl)]. Infusion of the angiotensin-II-antagonist saralasine led in one patient with Bartter's syndrome and one patient with decompensated hepatic cirrhosis, both of whom had a markedly raised plasma renin activity, to a fall in systolic and diastolic blood pressure. The results indicate that in normotensive patients a raised angiotensin II concentration in blood is haemodynamically important for the level of blood pressure if plasma renin activity is raised. In normotensives with normal plasma renin activity saralasine in the usual dosage (4.2 mug/kg-min) does not influence the blood pressure.", "PMID": 954592}
{"id": "pubmed23n0032_1209", "title": "[Frequency and pathogenis of thrombocytopenia in cardiac failure].", "content": "In 395 consecutively investigated patients with cardiac failure of varying aetiologythe platelet ocurt in venous blood was less than 100 000/mm(3) in 5.3% and below the 2s devaition (less than 136 000/mm (3)) in 19.2. The average platelet count of the whole group was 197 500 +/- 70 800/mm (3) which was significantly lower (P less than 0.001) than in normal controls (n = 128). In 6 patients a (51)Cr study of platelet kinetics was performed; the results support the conclusion that the faculatative thrombocytopenia in cardiac failure is mainly,but not exclusively, due to an increased uptake of platelets in the congested spleen.", "contents": "[Frequency and pathogenis of thrombocytopenia in cardiac failure]. In 395 consecutively investigated patients with cardiac failure of varying aetiologythe platelet ocurt in venous blood was less than 100 000/mm(3) in 5.3% and below the 2s devaition (less than 136 000/mm (3)) in 19.2. The average platelet count of the whole group was 197 500 +/- 70 800/mm (3) which was significantly lower (P less than 0.001) than in normal controls (n = 128). In 6 patients a (51)Cr study of platelet kinetics was performed; the results support the conclusion that the faculatative thrombocytopenia in cardiac failure is mainly,but not exclusively, due to an increased uptake of platelets in the congested spleen.", "PMID": 954598}
{"id": "pubmed23n0032_1210", "title": "[Aplastic anaemia and thymonal].", "content": "The combination of aplastic anaemia and thymona was observed in a 49-year old woman. Concomitant with severe normochromic anaemia and reticulocytopenia was a cell-rich bone marrow smear in which erythropoietic precursors were almost absent. Immunological phenomena could not be demonstrated. The erythropoietin level was maximally increased. Pathological anatomy typically revealed a predominantly spindle-cell thymonal with only minor capsular invasion.", "contents": "[Aplastic anaemia and thymonal]. The combination of aplastic anaemia and thymona was observed in a 49-year old woman. Concomitant with severe normochromic anaemia and reticulocytopenia was a cell-rich bone marrow smear in which erythropoietic precursors were almost absent. Immunological phenomena could not be demonstrated. The erythropoietin level was maximally increased. Pathological anatomy typically revealed a predominantly spindle-cell thymonal with only minor capsular invasion.", "PMID": 954599}
{"id": "pubmed23n0032_1211", "title": "Tinidazole: a review of its antiprotozoal activity and therapeutic efficacy.", "content": "Tinidazole, a synthetic imidazole derivative, has been used in the oral treatment of several protozoal infections - trichomoniasis, giardiasis and amoebiasis. Among the protozoal organisms inhibited by tinidazole are Trichomonas vaginalis, Trichomonas foetus, and Entamoeba histolytica. In vitro, tinidazole has been shown to possess antiprotozoal activity at least comparable to, and in some cases greater than, metronidazole. Tinidazole also has activity against some Gram-negative anaerobic bacilli, including Bacteroides spp. Following oral administration of a 2g dose, like metronidazole serum levels peak in about 2 hours but persist for longer. Any clinical significance of the longer plasma half-life (tinidazole 12.5h; metronidazole 7.3h) has yet to be demonstrated. Tinidazole is approximately 20% bound to plasma proteins. Only unchanged drug has been found in the plasma and urine of tinidazole-treated subjects, although metabolites have been detected in animal studies. A single 2g dose of tinidazole has been shown to be effective therapy in vaginal trichomoniasis and in urogenital trichomoniasis in males. Single-dose therapy in general offers advantages in regard to convenience, and in the treatment of a sexually transmissible disease such as trichomoniasis, single-dose therapy facilitates compliance of patient and sexual partner. In comparative studies, tinidazole, in both single-dose and traditional multiple-dose regimens, has been shown to be equivalent and often superior to other antitrichomonal agents, including metronidazole. In intestinal amoebiasis, tinidazole has been evaluated after both once-a-day and multiple daily dose regimens, with the former giving slightly better results. When both metronidazole and tinidazole were administered in multiple daily dose regimens, the two agents yielded similar cure rates; in one study fewer tinidazole-treated patients required a second course. Tinidazole has also been successful in some cases of amoebic liver abscess, but an advantage over metronidazole has not been demonstrated. Results in the treatment of giardiasis, especially with the single-dose regimen, are promising, and in one study, tinidazole proved effective in infections resistant to metronidazole. Even in large doses, tinidazole has been well tolerated, although rarely vomiting may occur and the patient may need to be re-treated with a multiple dose regimen.", "contents": "Tinidazole: a review of its antiprotozoal activity and therapeutic efficacy. Tinidazole, a synthetic imidazole derivative, has been used in the oral treatment of several protozoal infections - trichomoniasis, giardiasis and amoebiasis. Among the protozoal organisms inhibited by tinidazole are Trichomonas vaginalis, Trichomonas foetus, and Entamoeba histolytica. In vitro, tinidazole has been shown to possess antiprotozoal activity at least comparable to, and in some cases greater than, metronidazole. Tinidazole also has activity against some Gram-negative anaerobic bacilli, including Bacteroides spp. Following oral administration of a 2g dose, like metronidazole serum levels peak in about 2 hours but persist for longer. Any clinical significance of the longer plasma half-life (tinidazole 12.5h; metronidazole 7.3h) has yet to be demonstrated. Tinidazole is approximately 20% bound to plasma proteins. Only unchanged drug has been found in the plasma and urine of tinidazole-treated subjects, although metabolites have been detected in animal studies. A single 2g dose of tinidazole has been shown to be effective therapy in vaginal trichomoniasis and in urogenital trichomoniasis in males. Single-dose therapy in general offers advantages in regard to convenience, and in the treatment of a sexually transmissible disease such as trichomoniasis, single-dose therapy facilitates compliance of patient and sexual partner. In comparative studies, tinidazole, in both single-dose and traditional multiple-dose regimens, has been shown to be equivalent and often superior to other antitrichomonal agents, including metronidazole. In intestinal amoebiasis, tinidazole has been evaluated after both once-a-day and multiple daily dose regimens, with the former giving slightly better results. When both metronidazole and tinidazole were administered in multiple daily dose regimens, the two agents yielded similar cure rates; in one study fewer tinidazole-treated patients required a second course. Tinidazole has also been successful in some cases of amoebic liver abscess, but an advantage over metronidazole has not been demonstrated. Results in the treatment of giardiasis, especially with the single-dose regimen, are promising, and in one study, tinidazole proved effective in infections resistant to metronidazole. Even in large doses, tinidazole has been well tolerated, although rarely vomiting may occur and the patient may need to be re-treated with a multiple dose regimen.", "PMID": 954609}
{"id": "pubmed23n0032_1212", "title": "Human chorionic gonadotropin: acetylation of tyrosyls with N-acetylimidazole.", "content": "When human chorionic gonadotropin (hCG) was treated with a 20-fold molar excess of N-acetylimidazole in aqueous solution, two tyrosyls were acetylated, resulting in a 50% reduction in in vivo biological activity. With increased quantities of reagent, the number of tyrosyls acetylated increased but with no further decrease in the biological activity. In vitro biological activity (binding to hCG receptors) of the hormone was not affected at all. Acetylation in urea increased tyrosyl modification, accompanied by acetylation of some lysyls, yielding a product in which all seven tyrosyls were acetylated and both in vivo and in in vitro biological activities were completely abolished. Deacylation with hydroxylamine partially restored biological activity of some but not all of the modified products. When individual hCG subunits were treated with the same reagent, the number of tyrosyls acetylated in each subunit again increased with increasing amounts of reagent, up to three in the alpha subunit and two in the beta subunit in the absence of urea. The tyrosyls in the beta subunit appeared less reactive to the reagent than those in the alpha subunit. Subunits modified to these extents retained ability to recombine as examined by gel electrophoresis, but the recombined products varied considerably in both in vivo and in vitro biological activities. A completely tyrosyl-acetylated product of hCG-alpha did not combine with intact hCG-beta, while fully modified hCG-beta did with intact hCG-alpha.", "contents": "Human chorionic gonadotropin: acetylation of tyrosyls with N-acetylimidazole. When human chorionic gonadotropin (hCG) was treated with a 20-fold molar excess of N-acetylimidazole in aqueous solution, two tyrosyls were acetylated, resulting in a 50% reduction in in vivo biological activity. With increased quantities of reagent, the number of tyrosyls acetylated increased but with no further decrease in the biological activity. In vitro biological activity (binding to hCG receptors) of the hormone was not affected at all. Acetylation in urea increased tyrosyl modification, accompanied by acetylation of some lysyls, yielding a product in which all seven tyrosyls were acetylated and both in vivo and in in vitro biological activities were completely abolished. Deacylation with hydroxylamine partially restored biological activity of some but not all of the modified products. When individual hCG subunits were treated with the same reagent, the number of tyrosyls acetylated in each subunit again increased with increasing amounts of reagent, up to three in the alpha subunit and two in the beta subunit in the absence of urea. The tyrosyls in the beta subunit appeared less reactive to the reagent than those in the alpha subunit. Subunits modified to these extents retained ability to recombine as examined by gel electrophoresis, but the recombined products varied considerably in both in vivo and in vitro biological activities. A completely tyrosyl-acetylated product of hCG-alpha did not combine with intact hCG-beta, while fully modified hCG-beta did with intact hCG-alpha.", "PMID": 954635}
{"id": "pubmed23n0032_1213", "title": "Regulation of adenylate cyclase coupled beta-adrenergic receptors by beta-adrenergic catecholamines.", "content": "Injection of frogs with beta-adrenergic catecholamines produced a selective desensitization (loss of responsiveness) of the erythrocyte membrane adenylate cylase to subsequent stimulation in vitro by isoproterenol. Basal, prostaglandin E1- and fluoride-sensitive enzyme activities were unaffected. A 77% (p less than 0.001) decline in isoproterenol-responsive enzyme activity in the cells from the treated animals was observed with no change in the Km for isoproterenol stimulation of the enzyme (concentration causing 1/2 maximal enzyme activation). The decrease in catecholamine-sensitive adenylate cyclase was accompanied by a parallel 68% (p less than 0.001) fall in the apparent number of beta-adrenergic receptors in the erythrocyte membranes, assessed by (-) (3H)alprenolol binding studies. There was no change in the affinity of the receptor binding sites. The catecholamine-induced desensitization and fall in the beta-adrenergic receptor number were both concentration and time-dependent and displayed beta-adrenergic specificity. Isoproterenol was more potent in desensitizing cells and in lowering the receptor number than was norepinephrine. The beta-adrenergic antagonist propranolol, but not the alpha-adrenergic antagonist phentolamine, blocked the desensitizing effects of isoproterenol. Propranolol itself, however, did not cause desensitization. Cells became resensitized to the stimulatory effects of catecholamines, in association with a return in beta-receptor number, when propranolol was injected into previously desensitized animals. The changes in receptor number in membranes from desensitized and resensitized animals were also reflected in soluble receptor preparations. The protein synthesis inhibitor cycloheximide did not affect either desensitization, resensitization, or the changes in receptor number which accompanied the changes in adenylate cyclase sensitivity to catecholamines. These findings suggest that the chronic occupancy of beta-adrenergic receptors by beta-adrenergic agonists (but not antagonists) decreases the number of functional beta-adrenergic receptor binding sites and, hence, lowers the responsiveness of adenylate cylase to catecholamine stimulation. The lack of effort of cycloheximide on these regulatory effects suggests that \"inactivation\" and subsequent \"reactivation\" of the receptors, rather than changes in receptor turnover, are involved.", "contents": "Regulation of adenylate cyclase coupled beta-adrenergic receptors by beta-adrenergic catecholamines. Injection of frogs with beta-adrenergic catecholamines produced a selective desensitization (loss of responsiveness) of the erythrocyte membrane adenylate cylase to subsequent stimulation in vitro by isoproterenol. Basal, prostaglandin E1- and fluoride-sensitive enzyme activities were unaffected. A 77% (p less than 0.001) decline in isoproterenol-responsive enzyme activity in the cells from the treated animals was observed with no change in the Km for isoproterenol stimulation of the enzyme (concentration causing 1/2 maximal enzyme activation). The decrease in catecholamine-sensitive adenylate cyclase was accompanied by a parallel 68% (p less than 0.001) fall in the apparent number of beta-adrenergic receptors in the erythrocyte membranes, assessed by (-) (3H)alprenolol binding studies. There was no change in the affinity of the receptor binding sites. The catecholamine-induced desensitization and fall in the beta-adrenergic receptor number were both concentration and time-dependent and displayed beta-adrenergic specificity. Isoproterenol was more potent in desensitizing cells and in lowering the receptor number than was norepinephrine. The beta-adrenergic antagonist propranolol, but not the alpha-adrenergic antagonist phentolamine, blocked the desensitizing effects of isoproterenol. Propranolol itself, however, did not cause desensitization. Cells became resensitized to the stimulatory effects of catecholamines, in association with a return in beta-receptor number, when propranolol was injected into previously desensitized animals. The changes in receptor number in membranes from desensitized and resensitized animals were also reflected in soluble receptor preparations. The protein synthesis inhibitor cycloheximide did not affect either desensitization, resensitization, or the changes in receptor number which accompanied the changes in adenylate cyclase sensitivity to catecholamines. These findings suggest that the chronic occupancy of beta-adrenergic receptors by beta-adrenergic agonists (but not antagonists) decreases the number of functional beta-adrenergic receptor binding sites and, hence, lowers the responsiveness of adenylate cylase to catecholamine stimulation. The lack of effort of cycloheximide on these regulatory effects suggests that \"inactivation\" and subsequent \"reactivation\" of the receptors, rather than changes in receptor turnover, are involved.", "PMID": 954636}
{"id": "pubmed23n0032_1214", "title": "Studies on the antitesticular action of DL-6-(N-2-pipecolinomethyl)-5-hydroxy-indane (PMHI) in the rat.", "content": "Both prepubertal and adult rats were treated with a single oral dose of either 60 mg or 120 mg of dl-6-(N-pipecolinomethyl)-5-hydroxy indane maleate (PMHI) per kg of body weight. Their testicular weights were drastically reduced compared with those of the controls. A follow-up, beginning on the third day post-treatment and continuing for a period of 50 days, showed that the body weight growth of PMHI-treated rats was not retarded. The hormonal profile indicated that, except for FSH which showed a transitory elevation in PMHI-treated immature rats, the serum levels of LH, estrogen, and testosterone were indistinguishable from those of the controls. Testicular histology revealed that the spermatogenic process in PMHI-treated rats recovered at a dose-related rate. EM sections of testes of adult rats indicated that cytoplasmic vacuolation appeared in the Sertoli cells 5 h post-treatment. The consequent cascade of arrested spermiogenesis included abnormal acrosomal condensation of spermatids and sloughing of polynucleated spermatids. Some spermatocytes also seemed to be affected, but spermatogonia and Leydig cells remained intact. These results indicate the PMHI acts primarily on Sertoli cells and causes arrest in the spermiogenetic stage of the spermatids. At a higher and toxic dose of PMHI, however, the earlier germinal elements might also be affected, due to the extensive damage to the supporting Sertoli cells.", "contents": "Studies on the antitesticular action of DL-6-(N-2-pipecolinomethyl)-5-hydroxy-indane (PMHI) in the rat. Both prepubertal and adult rats were treated with a single oral dose of either 60 mg or 120 mg of dl-6-(N-pipecolinomethyl)-5-hydroxy indane maleate (PMHI) per kg of body weight. Their testicular weights were drastically reduced compared with those of the controls. A follow-up, beginning on the third day post-treatment and continuing for a period of 50 days, showed that the body weight growth of PMHI-treated rats was not retarded. The hormonal profile indicated that, except for FSH which showed a transitory elevation in PMHI-treated immature rats, the serum levels of LH, estrogen, and testosterone were indistinguishable from those of the controls. Testicular histology revealed that the spermatogenic process in PMHI-treated rats recovered at a dose-related rate. EM sections of testes of adult rats indicated that cytoplasmic vacuolation appeared in the Sertoli cells 5 h post-treatment. The consequent cascade of arrested spermiogenesis included abnormal acrosomal condensation of spermatids and sloughing of polynucleated spermatids. Some spermatocytes also seemed to be affected, but spermatogonia and Leydig cells remained intact. These results indicate the PMHI acts primarily on Sertoli cells and causes arrest in the spermiogenetic stage of the spermatids. At a higher and toxic dose of PMHI, however, the earlier germinal elements might also be affected, due to the extensive damage to the supporting Sertoli cells.", "PMID": 954637}
{"id": "pubmed23n0032_1215", "title": "Calcium metabolism during lactation: enhanced effects of thyrocalcitonin.", "content": "Lactating rats were compared with nonlactating controls, with regard to the intake and absorption of calcium, serum calcium level, and te protective effect of thyroacalcitonin (TC) against hypercalcemia and hyperphosphatemia. While consuming a commercial diet, intact, nonfasted lactating rats maintained a serum calcium level of approximately 9 mg/100 ml, which was 1 mg/100 ml lower than that of nonlactating controls. The level rose to that of the controls within one day after removal of the litters from the mother. Compared with nonlactating rats, lactating rats had a three-fold higher calcium intake and a six-fold higher rate of net absorption of calcium. After intragastric calcium (10 mg/100 g body wt) the increase in serum calcium was small (1 mg/100 ml) 2 h later in both groups of sham-operated rats but was markedly increased in thyroparathyroidectomized groups, with the lactating rats showing a significantly greater increase than the nonlactating rats. The injection of a small dose of porcine thyrocalcitonin completely counteracted this hypercalcemia in lactating rats, but did not have any effect on nonlactating controls. Protection by the thyroid gland against hyperphosphatemia after intragastric calcium also was significant in both lactating and nonlactating rats. The results show that TC is much more effective in lactating than in nonlactating rats, suggesting that TC may be of particular importance in lactation by restricting elevations of serum calcium and phosphorus levels after eating, thereby aiding in conservation of these ions.", "contents": "Calcium metabolism during lactation: enhanced effects of thyrocalcitonin. Lactating rats were compared with nonlactating controls, with regard to the intake and absorption of calcium, serum calcium level, and te protective effect of thyroacalcitonin (TC) against hypercalcemia and hyperphosphatemia. While consuming a commercial diet, intact, nonfasted lactating rats maintained a serum calcium level of approximately 9 mg/100 ml, which was 1 mg/100 ml lower than that of nonlactating controls. The level rose to that of the controls within one day after removal of the litters from the mother. Compared with nonlactating rats, lactating rats had a three-fold higher calcium intake and a six-fold higher rate of net absorption of calcium. After intragastric calcium (10 mg/100 g body wt) the increase in serum calcium was small (1 mg/100 ml) 2 h later in both groups of sham-operated rats but was markedly increased in thyroparathyroidectomized groups, with the lactating rats showing a significantly greater increase than the nonlactating rats. The injection of a small dose of porcine thyrocalcitonin completely counteracted this hypercalcemia in lactating rats, but did not have any effect on nonlactating controls. Protection by the thyroid gland against hyperphosphatemia after intragastric calcium also was significant in both lactating and nonlactating rats. The results show that TC is much more effective in lactating than in nonlactating rats, suggesting that TC may be of particular importance in lactation by restricting elevations of serum calcium and phosphorus levels after eating, thereby aiding in conservation of these ions.", "PMID": 954638}
{"id": "pubmed23n0032_1216", "title": "Studies on the transfer of steroid hormones across the blood-cerebrospinal fluid barrier in the Rhesus Monkey.", "content": "Indwelling canulae were placed in the lateral ventricles of the brains of six adult male rhesus monkeys, and the movement of estradiol-17beta (E2), testosterone (T), and 5alpha-dihydrotestosterone (DHT) across the blood-cerebrospinal fluid (CSF) barrier was measured. Serial samples of blood and CSF were collected every 30 minutes during a 6 hour infusion of the tritiated steroids, and the quantity of free steroid in the blood and CSF was determined by recrystallization to constant specific activity. During the course of the 6-hour infusion, the average CSF concentration of steroid, expressed as dpm/ml, was about 3.5% of the concurrent plasma level of E2, 1.6% of the concurrent plasma level of T, and 0.08% of the concurrent plasma level of DHT. It is proposed that these differences in steroid transfer can be attributed to differential binding of these steroids to testosterone-estrogen-binding globulin (TeBG) in plasma.", "contents": "Studies on the transfer of steroid hormones across the blood-cerebrospinal fluid barrier in the Rhesus Monkey. Indwelling canulae were placed in the lateral ventricles of the brains of six adult male rhesus monkeys, and the movement of estradiol-17beta (E2), testosterone (T), and 5alpha-dihydrotestosterone (DHT) across the blood-cerebrospinal fluid (CSF) barrier was measured. Serial samples of blood and CSF were collected every 30 minutes during a 6 hour infusion of the tritiated steroids, and the quantity of free steroid in the blood and CSF was determined by recrystallization to constant specific activity. During the course of the 6-hour infusion, the average CSF concentration of steroid, expressed as dpm/ml, was about 3.5% of the concurrent plasma level of E2, 1.6% of the concurrent plasma level of T, and 0.08% of the concurrent plasma level of DHT. It is proposed that these differences in steroid transfer can be attributed to differential binding of these steroids to testosterone-estrogen-binding globulin (TeBG) in plasma.", "PMID": 954639}
{"id": "pubmed23n0032_1217", "title": "Physiological cortisol levels block the inhibition of vascular reactivity produced by prolactin.", "content": "Cortisol in concentrations similar to the unbound levels of the hormone in human plasma can reverse the inhibition of vascular reactivity produced by prolactin. In the rat mesenteric vascular bed, cortisol alone in similar concentrations had no significant effect on the pressor responses to norepinephrine: the action of cortisol was seen only when prolactin was present. The relationships between the effects of different concentrations of prolactin and cortisol suggest that at some point there is a competitive interplay between the effects of the two hormones. There is indirect evidence that this interplay is at the level of prostaglandin synthesis or release. We suggest that cortisol has no effect on basal prostaglandin production but blocks the synthesis or release occurring in response to polypeptide hormone stimulation.", "contents": "Physiological cortisol levels block the inhibition of vascular reactivity produced by prolactin. Cortisol in concentrations similar to the unbound levels of the hormone in human plasma can reverse the inhibition of vascular reactivity produced by prolactin. In the rat mesenteric vascular bed, cortisol alone in similar concentrations had no significant effect on the pressor responses to norepinephrine: the action of cortisol was seen only when prolactin was present. The relationships between the effects of different concentrations of prolactin and cortisol suggest that at some point there is a competitive interplay between the effects of the two hormones. There is indirect evidence that this interplay is at the level of prostaglandin synthesis or release. We suggest that cortisol has no effect on basal prostaglandin production but blocks the synthesis or release occurring in response to polypeptide hormone stimulation.", "PMID": 954640}
{"id": "pubmed23n0032_1218", "title": "Effects of intraventricular catecholamines on luteinizing hormone release in ovariectomized-steroid-primed rats.", "content": "The effects of intraventricular norepinephrine (NE) and dopamine (DA) were studied in the awake, freely behaving rat. In long-term ovariectomized, estrogen-progesterone-primed (OVE E2-P) animals, blood samples were taken via indwelling intra-atrial catheters before and after intraventricular infusion of either pH-adjusted saline, NE )5 mug, 15 mug, 20 mug), or DA (4 mug, 15 mug), and plasma LH was measured by radioimmunoassay. Under urethane anesthesia, records were made of the effects of intraventricular saline and NE on the electrical activity of the arcuate nucleus in the form of multi-unit spike activity. In unanesthetized animals, intraventricular NE caused marked changes in behavior. The typical response consisted of three phases: generalized activation (5-7 min), feeding (5-15 min), and sleep 1-2 h). DA exerted similar behavioral effects but without the marked sleep phase characteristic of the NE response. The effects of the catecholamines on LH output were significant increases in plasma LH levels for all NE doses tested (5 mug, p less than .025; 15 mug, p less than .05; 20 mug, p less than .005), while DA had no effect. The dynamics of the LH response to NEwere similar at all dosage levels, and the increase caused by 20 mug NE was found to be essentially equal to that induced by a quick intravenous infusion of 1.25 ng LHRH. Arcuate nucleus multi-unit spike activity (MUSA) showed a clear response to intraventricular NE at a dosage capable of stimulating the release of LH. In every case, the initial effect was a decrease in spike activity. These results, considered in relation to previous findings, suggest that NE may be stimulatory to neurons secreting LH-releasing hormone (LHRH). The decrease in arcuate nucleus MUSA in response to NE implies that certain elements of this nucleus are inhibited during LH release, perhaps the dopaminergic tuberoinfundibular neurons.", "contents": "Effects of intraventricular catecholamines on luteinizing hormone release in ovariectomized-steroid-primed rats. The effects of intraventricular norepinephrine (NE) and dopamine (DA) were studied in the awake, freely behaving rat. In long-term ovariectomized, estrogen-progesterone-primed (OVE E2-P) animals, blood samples were taken via indwelling intra-atrial catheters before and after intraventricular infusion of either pH-adjusted saline, NE )5 mug, 15 mug, 20 mug), or DA (4 mug, 15 mug), and plasma LH was measured by radioimmunoassay. Under urethane anesthesia, records were made of the effects of intraventricular saline and NE on the electrical activity of the arcuate nucleus in the form of multi-unit spike activity. In unanesthetized animals, intraventricular NE caused marked changes in behavior. The typical response consisted of three phases: generalized activation (5-7 min), feeding (5-15 min), and sleep 1-2 h). DA exerted similar behavioral effects but without the marked sleep phase characteristic of the NE response. The effects of the catecholamines on LH output were significant increases in plasma LH levels for all NE doses tested (5 mug, p less than .025; 15 mug, p less than .05; 20 mug, p less than .005), while DA had no effect. The dynamics of the LH response to NEwere similar at all dosage levels, and the increase caused by 20 mug NE was found to be essentially equal to that induced by a quick intravenous infusion of 1.25 ng LHRH. Arcuate nucleus multi-unit spike activity (MUSA) showed a clear response to intraventricular NE at a dosage capable of stimulating the release of LH. In every case, the initial effect was a decrease in spike activity. These results, considered in relation to previous findings, suggest that NE may be stimulatory to neurons secreting LH-releasing hormone (LHRH). The decrease in arcuate nucleus MUSA in response to NE implies that certain elements of this nucleus are inhibited during LH release, perhaps the dopaminergic tuberoinfundibular neurons.", "PMID": 954641}
{"id": "pubmed23n0032_1219", "title": "Evidence for the simultaneous uptake and release of certain estrogens by the splanchnic area in the dog.", "content": "Splanchnic extractions of total radioactivity, unconjugated radioactive estrogens, radioactive estrone glucosiduronate, and radioactive estradiol-17beta glucosiduronate(s) were determined in dogs following the infusion of (3H)estrone glucosiduronate and of (3H)estrone. Splanchnic extractions of radioactive estradiol-17alpha-3-glucosiduronate were determined after (3H)estrone glucosiduronate infusion. The mean splanchnic extractions of total radioactivity after (3H)estrone glucosiduronate and (3H)estrone infusions were positive and similar (42.2 +/- 2.4% (SE) and 34.9 +/- 2.4% (SE), respectively). The mean splanchnic extraction of unconjugated radioactive estrogens after (3H)estrone glucosiduronate infusion (39.3 +/- 2.6%) was significantly less than that after (3H)estrone infusion (86.4 +/- 2.3%). The difference was best explained by different rates of formation of unconjugated from conjugated estrogens, suggesting, in turn, that simultaneous uptake and formation of unconjugated estrogens occur in the splanchnic area. The mean splanchnic extraction of extradiol-17alpha-3-glucosiduronate was 33 +/- 3.9%. The mean splanchnic extraction of radioactive estrone glucosiduronate after (3H)estrone glucosiduronate infusion was positive (54.7 +/- 2.3%) and after (3H)estrone infusion was negative (-50.8 +/-8.8%. The mean splanchnic extraction of estradiol-17beta glucosiduronate(s) was also positive (32 +/- 2.4%) after (3H)estrone glucosiduronate infusion and negative (-37.9 +/- 11.0%) after (3H)estrone infusion. These values are taken to indicate that simultaneous uptake and formation (from unconjugated estrogens) of both estrone glucosiduronate and estradiol-17beta glucosiduronate(s) occur in the splanchnic area, and that after (3H)estrone infusion, the rate of formation greatly exceeds t", "contents": "Evidence for the simultaneous uptake and release of certain estrogens by the splanchnic area in the dog. Splanchnic extractions of total radioactivity, unconjugated radioactive estrogens, radioactive estrone glucosiduronate, and radioactive estradiol-17beta glucosiduronate(s) were determined in dogs following the infusion of (3H)estrone glucosiduronate and of (3H)estrone. Splanchnic extractions of radioactive estradiol-17alpha-3-glucosiduronate were determined after (3H)estrone glucosiduronate infusion. The mean splanchnic extractions of total radioactivity after (3H)estrone glucosiduronate and (3H)estrone infusions were positive and similar (42.2 +/- 2.4% (SE) and 34.9 +/- 2.4% (SE), respectively). The mean splanchnic extraction of unconjugated radioactive estrogens after (3H)estrone glucosiduronate infusion (39.3 +/- 2.6%) was significantly less than that after (3H)estrone infusion (86.4 +/- 2.3%). The difference was best explained by different rates of formation of unconjugated from conjugated estrogens, suggesting, in turn, that simultaneous uptake and formation of unconjugated estrogens occur in the splanchnic area. The mean splanchnic extraction of extradiol-17alpha-3-glucosiduronate was 33 +/- 3.9%. The mean splanchnic extraction of radioactive estrone glucosiduronate after (3H)estrone glucosiduronate infusion was positive (54.7 +/- 2.3%) and after (3H)estrone infusion was negative (-50.8 +/-8.8%. The mean splanchnic extraction of estradiol-17beta glucosiduronate(s) was also positive (32 +/- 2.4%) after (3H)estrone glucosiduronate infusion and negative (-37.9 +/- 11.0%) after (3H)estrone infusion. These values are taken to indicate that simultaneous uptake and formation (from unconjugated estrogens) of both estrone glucosiduronate and estradiol-17beta glucosiduronate(s) occur in the splanchnic area, and that after (3H)estrone infusion, the rate of formation greatly exceeds t", "PMID": 954642}
{"id": "pubmed23n0032_1220", "title": "Relative biologic activities of human and bovine parathyroid hormones and their synthetic, NH2-terminal (1-34) peptides, as evaluated in vitro with renal cortical adenylate cyclase obtained from three different species.", "content": "The relative biologic activities of native human parathyroid hormone, hPTH (1-84), native bovine parathyroid hormone, bPTH (1-84), and their respective synthetic, NH2-terminal, biologically-active (1-34) fragments were compared in vitro using adenylate cyclase preparations from human, chicken, and rat renal cortex. The concentrations of the hormones required for half-maximal stimulation of the enzymes were determined from dose response curves. bPTH (1-84) had greater apparent activity than hPTH (1-84), using rat or chicken renal adenylate cyclase, but, with human renal adenylate cyclase, the apparent activities of the two hormones were equal. Synthetic hPTH (1-34) possessed about 1/10 of the apparent activity of hPTH (1-84) in all three adenylate cyclase systems. However, (GLU22)bPTH (1-34) was about equal inapparent activity to bPTH (1-84) in the three systems. We propose that different rates of hormone degradation at or near renal receptor sites may be responsible for the dependence of the relative biologic activity on the assay system used. In the case of hPTH a peptide chain longer than (1-34) may be required for the full biologic activity of the hormone...", "contents": "Relative biologic activities of human and bovine parathyroid hormones and their synthetic, NH2-terminal (1-34) peptides, as evaluated in vitro with renal cortical adenylate cyclase obtained from three different species. The relative biologic activities of native human parathyroid hormone, hPTH (1-84), native bovine parathyroid hormone, bPTH (1-84), and their respective synthetic, NH2-terminal, biologically-active (1-34) fragments were compared in vitro using adenylate cyclase preparations from human, chicken, and rat renal cortex. The concentrations of the hormones required for half-maximal stimulation of the enzymes were determined from dose response curves. bPTH (1-84) had greater apparent activity than hPTH (1-84), using rat or chicken renal adenylate cyclase, but, with human renal adenylate cyclase, the apparent activities of the two hormones were equal. Synthetic hPTH (1-34) possessed about 1/10 of the apparent activity of hPTH (1-84) in all three adenylate cyclase systems. However, (GLU22)bPTH (1-34) was about equal inapparent activity to bPTH (1-84) in the three systems. We propose that different rates of hormone degradation at or near renal receptor sites may be responsible for the dependence of the relative biologic activity on the assay system used. In the case of hPTH a peptide chain longer than (1-34) may be required for the full biologic activity of the hormone...", "PMID": 954643}
{"id": "pubmed23n0032_1221", "title": "Effect of suckling on plasma prolactin and hypothalamic monoamine levels in the rat.", "content": "In lactating rats that were separated from their pups for 8 hours, suckling induced a rapid rise in plasma prolactin levels. Correlatively, it induced a depletion in dopamine (DA) and serotonin (5-HT) Concentrations within the hypothalamus as early as 5 min after the onset of suckling. This depletion lasted as long as the stimulus was maintained. A parallel increase in the level of the metabolite of 5-HT, 5-hydroxyindolacetic acid (5-HIAA), was observed. Under these conditons, the ratio of 5-HIAA/5-HT is augmented. This ratio is a good index of turnover of the transmitter. Suckling affected neither the content of noradrenaline (NA) in the hypothalamus nor the levels of any amine tested in the cerebral cortex. When pups were separated from their mother for 24 hours, suckling no longer resulted in changes in either plasma prolactin levels or hypothalamic DA, 5-HT, or 5-HIAA concentrations. We have concluded that the activation of a discrete system of 5-HT containing neurons is associated with the triggering effect of suckling on prolactin release.", "contents": "Effect of suckling on plasma prolactin and hypothalamic monoamine levels in the rat. In lactating rats that were separated from their pups for 8 hours, suckling induced a rapid rise in plasma prolactin levels. Correlatively, it induced a depletion in dopamine (DA) and serotonin (5-HT) Concentrations within the hypothalamus as early as 5 min after the onset of suckling. This depletion lasted as long as the stimulus was maintained. A parallel increase in the level of the metabolite of 5-HT, 5-hydroxyindolacetic acid (5-HIAA), was observed. Under these conditons, the ratio of 5-HIAA/5-HT is augmented. This ratio is a good index of turnover of the transmitter. Suckling affected neither the content of noradrenaline (NA) in the hypothalamus nor the levels of any amine tested in the cerebral cortex. When pups were separated from their mother for 24 hours, suckling no longer resulted in changes in either plasma prolactin levels or hypothalamic DA, 5-HT, or 5-HIAA concentrations. We have concluded that the activation of a discrete system of 5-HT containing neurons is associated with the triggering effect of suckling on prolactin release.", "PMID": 954644}
{"id": "pubmed23n0032_1222", "title": "Cortisol and GH responses to D- and L-amphetamine in monkeys.", "content": "Equimolar doses of D-amphetamine and L-amphetamine, and a water placebo were injected intravenously on different days into rhesus monkeys, and their plasma cortisol and GH responses were determined over a 3-hour period. D- and L-amphetamine (but not the placebo) equally suppressed plasma cortisol concentration and equally increased plasma GH concentration. Pretreatment of the monkeys with large doses of pimozide, a specific dopamine receptor blocker, did not appear to block the hormonal responses to either isomer of amphetamine. The results suggest that the hormonal responses to both D- and L-amphetamine were mediated by noradrenergic neurones stimulatory to GH and inhibitory to ACTH, and that D- and L-amphetamine exert equipotent noradrenergic effects in the neuroendocrine system.", "contents": "Cortisol and GH responses to D- and L-amphetamine in monkeys. Equimolar doses of D-amphetamine and L-amphetamine, and a water placebo were injected intravenously on different days into rhesus monkeys, and their plasma cortisol and GH responses were determined over a 3-hour period. D- and L-amphetamine (but not the placebo) equally suppressed plasma cortisol concentration and equally increased plasma GH concentration. Pretreatment of the monkeys with large doses of pimozide, a specific dopamine receptor blocker, did not appear to block the hormonal responses to either isomer of amphetamine. The results suggest that the hormonal responses to both D- and L-amphetamine were mediated by noradrenergic neurones stimulatory to GH and inhibitory to ACTH, and that D- and L-amphetamine exert equipotent noradrenergic effects in the neuroendocrine system.", "PMID": 954645}
{"id": "pubmed23n0032_1223", "title": "The metabolism of cortisol by term baboon neonates (Papio papio).", "content": "The metabolism of iv administered (4-14C)cortisol (F) was examined in 3 female, spontaneously delivered, term baboons less than 24th old. Sixty and 80% of 14C was recovered in urine within 24 and 68 h,respectively. The distribution of urinary 14C was 44.7% unconjugated, 18.1% glucuronoside, 3.3% sulfate, and 24% unextractable with ethyl acetate. The metabolites were isolated by chromatography and crystallization. Eight per cent of unconjugated and 60% of glucuronoside metabolites were more polar than the cortols, the majority being unknown I (Rf 0.15), and unknown II (Rf 0.35), (csf., 6beta-ol-F), Rf 0.44, ethyl acetate-chloroofrmmethanol-water, 25:75:50:50). Unconjugated cortisol plus cortisone (E) represented less than 1% or urinary 14C and tetrahydrocortisone (THE) glucuronoside represented 1.2%. Excretion of tetrahydrocortisol (THF) and products of side-chain cleavage were negligible. Excretion of 20beta-hydroxy metabolites and 6beta-ol-F was less than or equal to 5% of urinary 14C. The cortisol production rate (mean +/- SE) calculated from the specific activity of THE was 4.95 +/- 1.92 mg/day. The glucuronoside/unconjugated 14C-ratio (0.4) contrasts with those previously reported in nonpregnant (4.0), pregnant (1.0), and postpartum (1.3) animals, indicating that the metabolic pattern in newborns is an exaggeration of that in pregnancy. In neonates, unknown I and II compensate quantitatively for decreased glucuronoside excretion. Unknowns I and II are derivatives of THF and THE, suggesting that increased hydroxylase or deficient glucuronyl transferase, rather than impaired delta4-reductase, is responsible for decreased glucuronoside excretion. The low F production rate, reduced glucuronoside formation, and increase in highly polar compounds relative to nonpregnant adults resemble the situation in humans. However, the reduction in glucuronosides is compensated for, quantitatively, by highly polar metabolites, which are extractable from baboon urine with ethyl acetate but are nonextractable from the urine fo human neonates.", "contents": "The metabolism of cortisol by term baboon neonates (Papio papio). The metabolism of iv administered (4-14C)cortisol (F) was examined in 3 female, spontaneously delivered, term baboons less than 24th old. Sixty and 80% of 14C was recovered in urine within 24 and 68 h,respectively. The distribution of urinary 14C was 44.7% unconjugated, 18.1% glucuronoside, 3.3% sulfate, and 24% unextractable with ethyl acetate. The metabolites were isolated by chromatography and crystallization. Eight per cent of unconjugated and 60% of glucuronoside metabolites were more polar than the cortols, the majority being unknown I (Rf 0.15), and unknown II (Rf 0.35), (csf., 6beta-ol-F), Rf 0.44, ethyl acetate-chloroofrmmethanol-water, 25:75:50:50). Unconjugated cortisol plus cortisone (E) represented less than 1% or urinary 14C and tetrahydrocortisone (THE) glucuronoside represented 1.2%. Excretion of tetrahydrocortisol (THF) and products of side-chain cleavage were negligible. Excretion of 20beta-hydroxy metabolites and 6beta-ol-F was less than or equal to 5% of urinary 14C. The cortisol production rate (mean +/- SE) calculated from the specific activity of THE was 4.95 +/- 1.92 mg/day. The glucuronoside/unconjugated 14C-ratio (0.4) contrasts with those previously reported in nonpregnant (4.0), pregnant (1.0), and postpartum (1.3) animals, indicating that the metabolic pattern in newborns is an exaggeration of that in pregnancy. In neonates, unknown I and II compensate quantitatively for decreased glucuronoside excretion. Unknowns I and II are derivatives of THF and THE, suggesting that increased hydroxylase or deficient glucuronyl transferase, rather than impaired delta4-reductase, is responsible for decreased glucuronoside excretion. The low F production rate, reduced glucuronoside formation, and increase in highly polar compounds relative to nonpregnant adults resemble the situation in humans. However, the reduction in glucuronosides is compensated for, quantitatively, by highly polar metabolites, which are extractable from baboon urine with ethyl acetate but are nonextractable from the urine fo human neonates.", "PMID": 954646}
{"id": "pubmed23n0032_1224", "title": "The effects of castration and treatment with testosterone on the biliary excretion of (3H)aldosterone in rats.", "content": "The rate of excretion of aldosterone radiometabolites into the bile duct cannulation, and the intravenous injection of (3H)aldosterone, was demonstrated to be markedly increased in male rats following castration. In 1 h, 72% of the injected 3H-radioactivity was excreted in the bile of castrated male rats compared with 26% in the intact male control rats. Castration of the males led to the increased biliary excretion of aldosterone metabolites and the elimination of the sex-dependence of this process in rats. The ovariectomy of female rats did not substantially increase the rate of excretion of aldosterone metabolites via the bile. Castrated male rats treated with testosterone excreted aldosterone metabolites into the bile at a slower rate. A similar treatment of ovariectomized female rats with testosterone also significantly slowed the rate of biliary excretion of the aldosterone metabolites. These findings suggest that the presence of androgens plays an important role in regulating the routes of hepatic metabolism of aldosterone and the rates of clearance of aldosterone and its metabolites from the plasma into the bile of rats.", "contents": "The effects of castration and treatment with testosterone on the biliary excretion of (3H)aldosterone in rats. The rate of excretion of aldosterone radiometabolites into the bile duct cannulation, and the intravenous injection of (3H)aldosterone, was demonstrated to be markedly increased in male rats following castration. In 1 h, 72% of the injected 3H-radioactivity was excreted in the bile of castrated male rats compared with 26% in the intact male control rats. Castration of the males led to the increased biliary excretion of aldosterone metabolites and the elimination of the sex-dependence of this process in rats. The ovariectomy of female rats did not substantially increase the rate of excretion of aldosterone metabolites via the bile. Castrated male rats treated with testosterone excreted aldosterone metabolites into the bile at a slower rate. A similar treatment of ovariectomized female rats with testosterone also significantly slowed the rate of biliary excretion of the aldosterone metabolites. These findings suggest that the presence of androgens plays an important role in regulating the routes of hepatic metabolism of aldosterone and the rates of clearance of aldosterone and its metabolites from the plasma into the bile of rats.", "PMID": 954647}
{"id": "pubmed23n0032_1225", "title": "Metabolism of single-component and high-molecular-weight radioactive insulin in rats.", "content": "Single component (125I)iodoinsulin injected in rats was converted into a high-molecular-weight form. This form of radioactive insulin was shown to be metabolized significantly more slowly and to exhibit a more prolonged half-life than the single-component radioactive insulin. When injected in rats, it remained predominantly in the high-molecular-weight form without conversion into free, single-component insulin. A significant amount of radioactivity was shown to be in the kidneys of rats following the injection of single-component radioactive insulin. The radioactivity of kidney homogenates was shown to exhibiti molecular sizes similar to those of single-component insulin, gamma globulin, and insulin degradation products. The injection of the high-molecular-weight form of radioactive insulin did not produce a significant accumulation of radioactivity in the kidneys of the rats; the radioactivity remained primarily in the blood of the rats, a finding consistent with the observed prolonged half-life of this form of radioactive insulin. The radioactivity in the urine of the animals injected with the single component or the high-molecular-weight radioactive insulin was present only in the region corresponding to degradation products.", "contents": "Metabolism of single-component and high-molecular-weight radioactive insulin in rats. Single component (125I)iodoinsulin injected in rats was converted into a high-molecular-weight form. This form of radioactive insulin was shown to be metabolized significantly more slowly and to exhibit a more prolonged half-life than the single-component radioactive insulin. When injected in rats, it remained predominantly in the high-molecular-weight form without conversion into free, single-component insulin. A significant amount of radioactivity was shown to be in the kidneys of rats following the injection of single-component radioactive insulin. The radioactivity of kidney homogenates was shown to exhibiti molecular sizes similar to those of single-component insulin, gamma globulin, and insulin degradation products. The injection of the high-molecular-weight form of radioactive insulin did not produce a significant accumulation of radioactivity in the kidneys of the rats; the radioactivity remained primarily in the blood of the rats, a finding consistent with the observed prolonged half-life of this form of radioactive insulin. The radioactivity in the urine of the animals injected with the single component or the high-molecular-weight radioactive insulin was present only in the region corresponding to degradation products.", "PMID": 954648}
{"id": "pubmed23n0032_1226", "title": "Effects of prostaglandins on RNA and casein synthesis in mammary gland explants of mice.", "content": "Prostaglandins B2, E2, and F2alpha were found to stimulate RNA synthesis in mammary gland explants of mice in a prolactin-like manner. These effects were non-additive to maximally stimulatory concentrations of prolacin. Also, time-course studies showed that the effects of these prostaglandins only became apparent following at 2-4 hour lag period. The effects of these prostaglandins were not observed in explants which were not pretreated with insulin plus hydrocortisone, i.e., in tissues not responsive to prolactin Prostaglandins E1, A2, A1, B1, and F1alpha had no effects on the rate of RNA synthesis, but each of these prostaglandins abolished the responsivitiy of the explants to prolactin. Since DBcAMP, theophylline, and indomethacin were found previously to suppress the aciton of prolactin on RNA synthesis, the effects of these agents on the stimulatory effects of prostaglandins B2, E2, and F2alpha were tested. The incubation of explants with 5 mM DBcAMP or 5 mM thophylline abolished the effects of these prostaglandins on RNA synthesis. But incubation with indomethacin did not affect the responses to these prostaglandins. Prolactin-like effects of prostaglandins on casein synthesis were not observed. Prostaglandins E1, A2, A1, B2, F1alpha and indomethacin did, however, abolish the prolactin stimulation of casein synthesis. Furthermore, the preincubation of explants with prostaglandins B2, E2, F2alpha or arachidonic acid significantly shortened the time of onset of the prolactin stimulation of casein synthesis. The results are discussed with regard to a possible role of the prostaglandins as mediators for the actions of prolactin in the mammary gland.", "contents": "Effects of prostaglandins on RNA and casein synthesis in mammary gland explants of mice. Prostaglandins B2, E2, and F2alpha were found to stimulate RNA synthesis in mammary gland explants of mice in a prolactin-like manner. These effects were non-additive to maximally stimulatory concentrations of prolacin. Also, time-course studies showed that the effects of these prostaglandins only became apparent following at 2-4 hour lag period. The effects of these prostaglandins were not observed in explants which were not pretreated with insulin plus hydrocortisone, i.e., in tissues not responsive to prolactin Prostaglandins E1, A2, A1, B1, and F1alpha had no effects on the rate of RNA synthesis, but each of these prostaglandins abolished the responsivitiy of the explants to prolactin. Since DBcAMP, theophylline, and indomethacin were found previously to suppress the aciton of prolactin on RNA synthesis, the effects of these agents on the stimulatory effects of prostaglandins B2, E2, and F2alpha were tested. The incubation of explants with 5 mM DBcAMP or 5 mM thophylline abolished the effects of these prostaglandins on RNA synthesis. But incubation with indomethacin did not affect the responses to these prostaglandins. Prolactin-like effects of prostaglandins on casein synthesis were not observed. Prostaglandins E1, A2, A1, B2, F1alpha and indomethacin did, however, abolish the prolactin stimulation of casein synthesis. Furthermore, the preincubation of explants with prostaglandins B2, E2, F2alpha or arachidonic acid significantly shortened the time of onset of the prolactin stimulation of casein synthesis. The results are discussed with regard to a possible role of the prostaglandins as mediators for the actions of prolactin in the mammary gland.", "PMID": 954649}
{"id": "pubmed23n0032_1227", "title": "Participation of serotonin in the phasic release of LH. I. Evidence from pharmacological experiments.", "content": "Subcutaneous implantation of a silastic tubing containing crystalline estradiol in castrated female rats results in a circadian rhythm of LH release. Under such conditions, blockade of serotonin (5-HT) biosynthesis by p-chlorophenylalanine (PCPA) completely inhibits the afternoon elevation of plasma LH. Gonadotropin peaks remain inhibited as long as the concentration of the transmitter is effectively depleted. Intraperitoneal administration or intraventricular infusion of minute doses of the immediate precursor of the amine, 5-hydroxytryptophan (5-HTP), results in the reappearance of the next afternoon rise of plasma gonadotropin, whenever hypothalamic levels of 5-hydroxyindole acetic acid (5-HIAA), the main metabolite of 5-HT, are significantly increased over their value in PGPA-treated animals. The administration of methiothepin, a 5-HT receptor blocker, 9 hours of more prior to the next expected LH rise, similarly inhibits the cycle, whereas a dopamine receptor inhibitor has no effect under the same time conditions. We have concluded that serotoninergic neuron systems can have a positive, permissive effect on the transfer of neural information resulting in phasic gonadotropin release; this action of the amine is different from, but not contradictory to, the known inhibitory effect of 5-HT on the release of LHRH from the median eminence.", "contents": "Participation of serotonin in the phasic release of LH. I. Evidence from pharmacological experiments. Subcutaneous implantation of a silastic tubing containing crystalline estradiol in castrated female rats results in a circadian rhythm of LH release. Under such conditions, blockade of serotonin (5-HT) biosynthesis by p-chlorophenylalanine (PCPA) completely inhibits the afternoon elevation of plasma LH. Gonadotropin peaks remain inhibited as long as the concentration of the transmitter is effectively depleted. Intraperitoneal administration or intraventricular infusion of minute doses of the immediate precursor of the amine, 5-hydroxytryptophan (5-HTP), results in the reappearance of the next afternoon rise of plasma gonadotropin, whenever hypothalamic levels of 5-hydroxyindole acetic acid (5-HIAA), the main metabolite of 5-HT, are significantly increased over their value in PGPA-treated animals. The administration of methiothepin, a 5-HT receptor blocker, 9 hours of more prior to the next expected LH rise, similarly inhibits the cycle, whereas a dopamine receptor inhibitor has no effect under the same time conditions. We have concluded that serotoninergic neuron systems can have a positive, permissive effect on the transfer of neural information resulting in phasic gonadotropin release; this action of the amine is different from, but not contradictory to, the known inhibitory effect of 5-HT on the release of LHRH from the median eminence.", "PMID": 954650}
{"id": "pubmed23n0032_1228", "title": "Metabolic clearance and secretion rates of porcine growth hormone in genetically lean and obese swine.", "content": "The metabolic clearance rate (MCR) and the secretion rate (SR) of porcine growth hormone (pGH) have been examined in swine rendered genetically either lean or obese after 18 generations of selection for or against backfat thickness. At 15 weeks of age (when the muscle:fat ratio was greater than 1) the mean half-life (t1/2), MCR, and SR, for the obese, control, and lean swine were: t1/2 = 7.4, 8.9, and 9.8 min; MCR = 341, 279, and 158 ml/min; SR = 907, 802, and 520 ng/min, respectively. At 90 kg body weight (when muscle:fat ratio was less than 1, and the age was about 30 weeks) the data for obese, control, and lean swine were: t1/2 = 11.3, 12.0, and 11.7 min; MCR =305, 280, and 336 ml/min; SR= 535, 626, and 932 ng/min, respectively. The t1/2, MCR, and SR were not significantly different among the obese, control, and lean swine at either 15 weeks or 90 kg body weight. Comparing the two stages of development, the younger swine (15 weeks of age) had a shorter t1/2 (P less than .01), and secreted and cleared more pGH on a per kg body weight basis (P less than .05) than the older swine (90 kg bodyweight, about 30 weeks of age). However, the results suggest that the selection of swine for either leanness or fatness for 18 generations did not alter the MCR and SR of pGH. In addition, the differences observed between the younger and older swine suggest that GH is cleared at a more rapid rate and more GH is available per unit of mass in the younger animals.", "contents": "Metabolic clearance and secretion rates of porcine growth hormone in genetically lean and obese swine. The metabolic clearance rate (MCR) and the secretion rate (SR) of porcine growth hormone (pGH) have been examined in swine rendered genetically either lean or obese after 18 generations of selection for or against backfat thickness. At 15 weeks of age (when the muscle:fat ratio was greater than 1) the mean half-life (t1/2), MCR, and SR, for the obese, control, and lean swine were: t1/2 = 7.4, 8.9, and 9.8 min; MCR = 341, 279, and 158 ml/min; SR = 907, 802, and 520 ng/min, respectively. At 90 kg body weight (when muscle:fat ratio was less than 1, and the age was about 30 weeks) the data for obese, control, and lean swine were: t1/2 = 11.3, 12.0, and 11.7 min; MCR =305, 280, and 336 ml/min; SR= 535, 626, and 932 ng/min, respectively. The t1/2, MCR, and SR were not significantly different among the obese, control, and lean swine at either 15 weeks or 90 kg body weight. Comparing the two stages of development, the younger swine (15 weeks of age) had a shorter t1/2 (P less than .01), and secreted and cleared more pGH on a per kg body weight basis (P less than .05) than the older swine (90 kg bodyweight, about 30 weeks of age). However, the results suggest that the selection of swine for either leanness or fatness for 18 generations did not alter the MCR and SR of pGH. In addition, the differences observed between the younger and older swine suggest that GH is cleared at a more rapid rate and more GH is available per unit of mass in the younger animals.", "PMID": 954651}
{"id": "pubmed23n0032_1229", "title": "Studies on a cell line from a functional rat thyroid tumor in continuous culture.", "content": "A clonal cell line derived from a functional and transplantable rat thyroid tumor was established in continuous monolayer culture by the use of enzymatic dissociation followed by an alternate culture-animal passage procedure. After being implanted backin the animal and again plated in culture, epithelial-like cells aggregated and rearranged themselves over the bottoms of dishes in structures resembling cross sections of a normal thyroid gland. The same morphology and growth pattern were maintained after innumerable subcultures and freeze/thawing periods. Cells grew with a population-doubling time of about 24 h in serum-supplemented synthetic medium. Cell monolayers stained with periodic acid-Schiff (PAS) showed a uniformly epithelial-like morphology; their cytoplasm contained abundant PAS-positive material that was resistant to enzymatic digestion with amylase. Thin-layer chromatography of acid-butanol cell extracts in primary and clonal cultures, followed by a specific and sensitive staining method for iodine compounds, demonstrated the presence of monoiodotyrosine (MIT), diiodotyrosine, and triiodothyronine-thyroxine. In contrast, in similar extracts obtained from a cultured liver cell line, the only iodinated amino acid was MIT. Thus, with regard to the above criteria, this cell line preserved specialized thyroid cell morphology and function.", "contents": "Studies on a cell line from a functional rat thyroid tumor in continuous culture. A clonal cell line derived from a functional and transplantable rat thyroid tumor was established in continuous monolayer culture by the use of enzymatic dissociation followed by an alternate culture-animal passage procedure. After being implanted backin the animal and again plated in culture, epithelial-like cells aggregated and rearranged themselves over the bottoms of dishes in structures resembling cross sections of a normal thyroid gland. The same morphology and growth pattern were maintained after innumerable subcultures and freeze/thawing periods. Cells grew with a population-doubling time of about 24 h in serum-supplemented synthetic medium. Cell monolayers stained with periodic acid-Schiff (PAS) showed a uniformly epithelial-like morphology; their cytoplasm contained abundant PAS-positive material that was resistant to enzymatic digestion with amylase. Thin-layer chromatography of acid-butanol cell extracts in primary and clonal cultures, followed by a specific and sensitive staining method for iodine compounds, demonstrated the presence of monoiodotyrosine (MIT), diiodotyrosine, and triiodothyronine-thyroxine. In contrast, in similar extracts obtained from a cultured liver cell line, the only iodinated amino acid was MIT. Thus, with regard to the above criteria, this cell line preserved specialized thyroid cell morphology and function.", "PMID": 954652}
{"id": "pubmed23n0032_1230", "title": "Circadian rhythms of serum renin activity and serum corticosterone, prolactin, and aldosterone concentrations in the male rat on normal and low-sodium diets.", "content": "To investigate the control of aldosterone secretion, non-stress levels of serum aldosterone, corticosterone, and prolactin, and renin activity were determined at 4-h intervals during 24-h light-dark cycles in adult male rats on regular and low-sodium diets. Circadian rhythms of plasma aldosterone, prolactin, and corticosterone concentrations and of serum renin activity were demonstrated during a regular sodium diet. When the rats were on a low-sodium diet, a circadian rhythm of serum corticosterone and aldosterone concentration was observed, but there was no circadian variation in serum renin activity or in serum prolactin concentration. Serum aldosterone concentration correlated with serum corticosterone concentration (r = 0.48) and serum renin activity (r = 0.36) during a low-sodium diet. Serum prolactin concentration did not correlate with serum aldosterone concentration or serum osmolality. These data are compatible with a role for renin and ACTH, but not for prolactin, in the modulation of aldosterone secretion in the rat.", "contents": "Circadian rhythms of serum renin activity and serum corticosterone, prolactin, and aldosterone concentrations in the male rat on normal and low-sodium diets. To investigate the control of aldosterone secretion, non-stress levels of serum aldosterone, corticosterone, and prolactin, and renin activity were determined at 4-h intervals during 24-h light-dark cycles in adult male rats on regular and low-sodium diets. Circadian rhythms of plasma aldosterone, prolactin, and corticosterone concentrations and of serum renin activity were demonstrated during a regular sodium diet. When the rats were on a low-sodium diet, a circadian rhythm of serum corticosterone and aldosterone concentration was observed, but there was no circadian variation in serum renin activity or in serum prolactin concentration. Serum aldosterone concentration correlated with serum corticosterone concentration (r = 0.48) and serum renin activity (r = 0.36) during a low-sodium diet. Serum prolactin concentration did not correlate with serum aldosterone concentration or serum osmolality. These data are compatible with a role for renin and ACTH, but not for prolactin, in the modulation of aldosterone secretion in the rat.", "PMID": 954653}
{"id": "pubmed23n0032_1231", "title": "Synthesis of 17beta-estradiol by isolated ovarian tissues of the pregnant rat: aromatization in the corpus luteum.", "content": "Corpora lutea from pregnant rats were incubated to determine their ability to produce 17beta-estradiol and to aromatize testosterone in vitro. Corpora lutea and non-luteal ovarian tissues were removed from rats on days 7, 15, and 22 of pregnancy, and these tissues were immediately frozen or incubated separately in medium 199 at 37 C in an atmosphere of 95% O2-5% CO2 for 4 h. 17Beta-Estradiol in tissue and medium were quantified by a highly specific radioimmunoassay. The estradiol content ivnariably increased in non-luteal tissues during incubation, while it decreased or remained the same in incubated corpora lutea. The synthesis in non-luteal tissues, which was 18 to 400-fold greter. The incubation of corpora lutea (5 to 25 mg of tissue) with testosterone (200 ng) on days 7, 15, and 22 of pregnancy resulted in a mean accumulation of 17beta-estradiol in medium of 2.5 x 103 pg/mg tissue, compared with a mean value of 6 pg/mg for luteal tissue removed from the same ovaries and incubated without testosterone. The incubation of corpora lutea from 15-day pregnant rats with (7alpha-3H)-testosterone resulted in 15% conversion to presumptive (7alpha-3H)17beta-estradiol, which was isolated identically to estradiol isolated for radioimmunoassay. Recrystallization to constant specific activity revealed a high degree of radiochemical purity (75%) of the isolated (3H)estradiol. Rat diaphragm muscle and rabbit corpora lutea did not aromatize testosterone to 17beta-estradiol in amounts detectable by radioirpora lutea in vitro is virtually diol by non-luteal ovarian tissues. However,the corpora lutea show a striking capacity to aromatize testosterone, which might explain the high estradiol content of the rat corpora lutea during pregnancy. The physiological significance of this aromatizing system and of 17beta-estradiol in the corpus luteum is unknown but may be related to the luteotropic action of estradiol in the pregnant rat.", "contents": "Synthesis of 17beta-estradiol by isolated ovarian tissues of the pregnant rat: aromatization in the corpus luteum. Corpora lutea from pregnant rats were incubated to determine their ability to produce 17beta-estradiol and to aromatize testosterone in vitro. Corpora lutea and non-luteal ovarian tissues were removed from rats on days 7, 15, and 22 of pregnancy, and these tissues were immediately frozen or incubated separately in medium 199 at 37 C in an atmosphere of 95% O2-5% CO2 for 4 h. 17Beta-Estradiol in tissue and medium were quantified by a highly specific radioimmunoassay. The estradiol content ivnariably increased in non-luteal tissues during incubation, while it decreased or remained the same in incubated corpora lutea. The synthesis in non-luteal tissues, which was 18 to 400-fold greter. The incubation of corpora lutea (5 to 25 mg of tissue) with testosterone (200 ng) on days 7, 15, and 22 of pregnancy resulted in a mean accumulation of 17beta-estradiol in medium of 2.5 x 103 pg/mg tissue, compared with a mean value of 6 pg/mg for luteal tissue removed from the same ovaries and incubated without testosterone. The incubation of corpora lutea from 15-day pregnant rats with (7alpha-3H)-testosterone resulted in 15% conversion to presumptive (7alpha-3H)17beta-estradiol, which was isolated identically to estradiol isolated for radioimmunoassay. Recrystallization to constant specific activity revealed a high degree of radiochemical purity (75%) of the isolated (3H)estradiol. Rat diaphragm muscle and rabbit corpora lutea did not aromatize testosterone to 17beta-estradiol in amounts detectable by radioirpora lutea in vitro is virtually diol by non-luteal ovarian tissues. However,the corpora lutea show a striking capacity to aromatize testosterone, which might explain the high estradiol content of the rat corpora lutea during pregnancy. The physiological significance of this aromatizing system and of 17beta-estradiol in the corpus luteum is unknown but may be related to the luteotropic action of estradiol in the pregnant rat.", "PMID": 954654}
{"id": "pubmed23n0032_1232", "title": "Cartilage sulfation and serum somatomedin in rats during and after cortisone-induced growth arrest.", "content": "The uptake of sulfate by rib cartilage in vitro and in vivo and the serum somatomedin activity by bioassay were determined in male rats during and after cortisone-induced growth arrest. Experimental treatment consisted of subcutaneous injections of cortisone acetate in a dose of 2.5 mg/rat/day for 4 days, beginning at 29 to 30 days of age in Buffalo rats, or 5 mg/rat/day for 4 or 5 days, beginning at 39 to 41 days of age in Long-Evans rats. Groups of hypophysectomized rats were studied in parallel in one experiment. The sulfate uptake in the controls declined linearly with increasing age in both the in vitro and the in vivo studies. Hypophysectomy resulted in a constant low level of sulfate uptake in vitro. At the end of cortisone treatment, the in vitro sulfate uptake was approximately midway between that of the hypophysectomized rats and that of controls; at 7 days recovery, it was at the control level; at 14 days it showed an additional rise above the control value; from 14 days to 35 days it declined parallel with but above the sulfate uptake of controls. The in vivo sulfate uptake was depressed by cortisone treatment. During recovery it approximately control values at recovery day 21. In succeeding recovery periods in vivo sulfate uptake remained at control levels. Serum somatomedin activity was significantly reduced during cortisone treatment; it returned to the control level by 21 days of recovery. The incubation of the cartilage of controls and cortisone-treated rats at 14 days of recovery with and without the presence of normal rat serum, cortisone recovery serum, or hypophysectomy serum resulted in significantly higher sulfate uptake in the cortisone-treated rat cartilage in each medium. These sera did not differ significantly in their stimulation of sulfate uptake in either cortisone recovery cartilage or control cartilage. Both treated and control cartilage had greater sulfate uptake with larger doses of serum added to the medium. The dose-response curves were parallel during treatment and early recovery; but the slopes of the dose-response curves of the cartilage of cortisone-treated rats were greater than those of the controls during late recovery. It is concluded that the increased in vitro sulfation after 14 days of recovery in cortisone-treated rats signifies a persistent alteration in cartilage metabolism. Normal in vivo sulfate uptake during that time may be the result of humoral controls. A mechanism other than the impairment of somatomedin production is probably involved in the failure of catch-up growth after glucocorticoid treatment in the rat.", "contents": "Cartilage sulfation and serum somatomedin in rats during and after cortisone-induced growth arrest. The uptake of sulfate by rib cartilage in vitro and in vivo and the serum somatomedin activity by bioassay were determined in male rats during and after cortisone-induced growth arrest. Experimental treatment consisted of subcutaneous injections of cortisone acetate in a dose of 2.5 mg/rat/day for 4 days, beginning at 29 to 30 days of age in Buffalo rats, or 5 mg/rat/day for 4 or 5 days, beginning at 39 to 41 days of age in Long-Evans rats. Groups of hypophysectomized rats were studied in parallel in one experiment. The sulfate uptake in the controls declined linearly with increasing age in both the in vitro and the in vivo studies. Hypophysectomy resulted in a constant low level of sulfate uptake in vitro. At the end of cortisone treatment, the in vitro sulfate uptake was approximately midway between that of the hypophysectomized rats and that of controls; at 7 days recovery, it was at the control level; at 14 days it showed an additional rise above the control value; from 14 days to 35 days it declined parallel with but above the sulfate uptake of controls. The in vivo sulfate uptake was depressed by cortisone treatment. During recovery it approximately control values at recovery day 21. In succeeding recovery periods in vivo sulfate uptake remained at control levels. Serum somatomedin activity was significantly reduced during cortisone treatment; it returned to the control level by 21 days of recovery. The incubation of the cartilage of controls and cortisone-treated rats at 14 days of recovery with and without the presence of normal rat serum, cortisone recovery serum, or hypophysectomy serum resulted in significantly higher sulfate uptake in the cortisone-treated rat cartilage in each medium. These sera did not differ significantly in their stimulation of sulfate uptake in either cortisone recovery cartilage or control cartilage. Both treated and control cartilage had greater sulfate uptake with larger doses of serum added to the medium. The dose-response curves were parallel during treatment and early recovery; but the slopes of the dose-response curves of the cartilage of cortisone-treated rats were greater than those of the controls during late recovery. It is concluded that the increased in vitro sulfation after 14 days of recovery in cortisone-treated rats signifies a persistent alteration in cartilage metabolism. Normal in vivo sulfate uptake during that time may be the result of humoral controls. A mechanism other than the impairment of somatomedin production is probably involved in the failure of catch-up growth after glucocorticoid treatment in the rat.", "PMID": 954655}
{"id": "pubmed23n0032_1233", "title": "The metabolic clearance rates and interconversion of cortisol and cortisone in pregnant and nonpregnant baboons.", "content": "Pregnancy alters the pattern of maternal cortisol (F) metabolism and increases the maternal serum cortisol-binding capacity (CBC) of baboons. To determine whether these changes are associated with alterations in F clearance,the metabolic clearance rate (MCR) and interconversion (p) of F and cortisone (E) were measured by continuous infusion of (3H)F and (14C)E in 9 regularly menstruating and 7 pregnant baboons (Papio papio). In nonpregnant animals, the values (X +/- SE) for MCR-E (488 +/- 48 1/day) were greater (P less than 0.001) than those for MCR-F (214 +/- 22 1/day). The p value for the conversion of E leads to F (62.8 +/- 4.7%) was greater (P less than 0.001) than that for the reaction F leads to E (41.6 +/- 3.7%), indicating that F formation is favored. Consistent with MCR-E greater than MCR-F, the per cent of F bound to proteins other than albumin (75 +/- 2) was greater (P less than 0.001) than the per cent of E bound (52 +/-3). The production rate  (MCR x peripheral concentration; mug/min) of F (55.1 +/- 7.9) was greater (P less than 0.001) than that of E (28.5 +/-3.9) with essentially all of the F being secreted directly (secretion rate 51.2 +/- 7.9 mug/min). Essentially all of the E produced was derived from circulating F, vitually none being secreted directly (secretion rate 4.6 +/- 3.9 mug/min). Pregnancy did not alter the MCR-F (190 +/- 23 1/day), MCR-E 525+/- 51 1/day), per cent of F (79 +/- 3), or per cent of E (49 +/-3) bound,or F (57.2 +/- 9.2 mug/min) or E (35.5 +/- 4.9 mug/min) production rates. CBC (mug F/100 ml) was significantly (P less than 0.01) elevated (25.3 +/- 2.3, nonpregnant vs 35.1 +/- u.6, pregnant). In addition, p E leads to F was increased (75.5 +/- 1.8%) as was p F leads to E (54.3 +/- 3.7%; P less than 0.01). We have concluded that the MCR-F during pregnancy is more dependent on alterations in maternal metabolism than on the increased serum CBC characteristic of gestation. We suggest that the latter factor may be important in regulating the physiologic levels of the other steroids which bind to it.", "contents": "The metabolic clearance rates and interconversion of cortisol and cortisone in pregnant and nonpregnant baboons. Pregnancy alters the pattern of maternal cortisol (F) metabolism and increases the maternal serum cortisol-binding capacity (CBC) of baboons. To determine whether these changes are associated with alterations in F clearance,the metabolic clearance rate (MCR) and interconversion (p) of F and cortisone (E) were measured by continuous infusion of (3H)F and (14C)E in 9 regularly menstruating and 7 pregnant baboons (Papio papio). In nonpregnant animals, the values (X +/- SE) for MCR-E (488 +/- 48 1/day) were greater (P less than 0.001) than those for MCR-F (214 +/- 22 1/day). The p value for the conversion of E leads to F (62.8 +/- 4.7%) was greater (P less than 0.001) than that for the reaction F leads to E (41.6 +/- 3.7%), indicating that F formation is favored. Consistent with MCR-E greater than MCR-F, the per cent of F bound to proteins other than albumin (75 +/- 2) was greater (P less than 0.001) than the per cent of E bound (52 +/-3). The production rate  (MCR x peripheral concentration; mug/min) of F (55.1 +/- 7.9) was greater (P less than 0.001) than that of E (28.5 +/-3.9) with essentially all of the F being secreted directly (secretion rate 51.2 +/- 7.9 mug/min). Essentially all of the E produced was derived from circulating F, vitually none being secreted directly (secretion rate 4.6 +/- 3.9 mug/min). Pregnancy did not alter the MCR-F (190 +/- 23 1/day), MCR-E 525+/- 51 1/day), per cent of F (79 +/- 3), or per cent of E (49 +/-3) bound,or F (57.2 +/- 9.2 mug/min) or E (35.5 +/- 4.9 mug/min) production rates. CBC (mug F/100 ml) was significantly (P less than 0.01) elevated (25.3 +/- 2.3, nonpregnant vs 35.1 +/- u.6, pregnant). In addition, p E leads to F was increased (75.5 +/- 1.8%) as was p F leads to E (54.3 +/- 3.7%; P less than 0.01). We have concluded that the MCR-F during pregnancy is more dependent on alterations in maternal metabolism than on the increased serum CBC characteristic of gestation. We suggest that the latter factor may be important in regulating the physiologic levels of the other steroids which bind to it.", "PMID": 954656}
{"id": "pubmed23n0032_1234", "title": "The formation of 5alpha-androstane-3alpha, 17beta-diol by dog prostate.", "content": "The 3-keto reduction of (1.2-3H)dihydrotestosterone to 3alpha and 3beta-androstanediol has been assessed in homogenates of 31 dog prostates that varied in size from less than 1 to greater than 15 g. The formation of both androstanediols was demonstrable in microsomes and cytosol, and both NADH and NADPH were effective cofactors for the two reactions. The formation of 3beta-androstanediol per unit weight of prostate or per mg protein did not show a clear-cut relation with the size fo the gland, but 3alpha-androstanediol formation per mg microsomal protein increased as a linear function with size so that the rates were on an average 10-fold greater in prostates greater than 15 g than in those less than 5 g in weight.", "contents": "The formation of 5alpha-androstane-3alpha, 17beta-diol by dog prostate. The 3-keto reduction of (1.2-3H)dihydrotestosterone to 3alpha and 3beta-androstanediol has been assessed in homogenates of 31 dog prostates that varied in size from less than 1 to greater than 15 g. The formation of both androstanediols was demonstrable in microsomes and cytosol, and both NADH and NADPH were effective cofactors for the two reactions. The formation of 3beta-androstanediol per unit weight of prostate or per mg protein did not show a clear-cut relation with the size fo the gland, but 3alpha-androstanediol formation per mg microsomal protein increased as a linear function with size so that the rates were on an average 10-fold greater in prostates greater than 15 g than in those less than 5 g in weight.", "PMID": 954657}
{"id": "pubmed23n0032_1235", "title": "Acute changes in norepinephrine content in the median eminence induced by orchidectomy or testosterone replacement.", "content": "Median eminence catecholamines were measured in individual samples from intact, castrated, or sham-operated male rats, in order to correlate changes in catecholamine content with the early post-castration rise in serum LH levels. Both norepinephrine (NE) and dopamine (DA) were measured by an enzymatic-isotopic assay and advantage was also taken of a method recently developed that makes it possible to obtain the median eminence free of the surrounding hypothalamus. Determinations were made at 4, 8, or 24 h after surgery. All animals were killed between 1400 and 1600 h. It was observed that castration increases the ME content of NE significantly at all the times studied, with peak values 8 h after orchidectomy. Sham operation induced a significant decrease in NE content in the ME 4 h after surgery. By 8 h the NE values had returned to intact control levels. Both castration and sham operation induced a significant decrease in DA levels 4 h after surgery. By 8 h, DA values had returned to intact control levels in both groups. When the NE levels in castrated rats had reached the highest value, serum LH values had not departed from control levels, indicating that the change in NE content in the ME takes place prior to the expected rise in LH. Testosterone replacement therapy (40 mug/100 gBW) of castrated rats blocked the increase in NE content observed 8 h after castration. Dopamine levels were not affected by this treatment. These results support the concept that the NE contained in median eminence catecholaminergic terminals participates in the feed-back regulation of LH secretion in the male rat, while DA is apparently not involved under the present conditions.", "contents": "Acute changes in norepinephrine content in the median eminence induced by orchidectomy or testosterone replacement. Median eminence catecholamines were measured in individual samples from intact, castrated, or sham-operated male rats, in order to correlate changes in catecholamine content with the early post-castration rise in serum LH levels. Both norepinephrine (NE) and dopamine (DA) were measured by an enzymatic-isotopic assay and advantage was also taken of a method recently developed that makes it possible to obtain the median eminence free of the surrounding hypothalamus. Determinations were made at 4, 8, or 24 h after surgery. All animals were killed between 1400 and 1600 h. It was observed that castration increases the ME content of NE significantly at all the times studied, with peak values 8 h after orchidectomy. Sham operation induced a significant decrease in NE content in the ME 4 h after surgery. By 8 h the NE values had returned to intact control levels. Both castration and sham operation induced a significant decrease in DA levels 4 h after surgery. By 8 h, DA values had returned to intact control levels in both groups. When the NE levels in castrated rats had reached the highest value, serum LH values had not departed from control levels, indicating that the change in NE content in the ME takes place prior to the expected rise in LH. Testosterone replacement therapy (40 mug/100 gBW) of castrated rats blocked the increase in NE content observed 8 h after castration. Dopamine levels were not affected by this treatment. These results support the concept that the NE contained in median eminence catecholaminergic terminals participates in the feed-back regulation of LH secretion in the male rat, while DA is apparently not involved under the present conditions.", "PMID": 954658}
{"id": "pubmed23n0032_1236", "title": "Studies on the extra-hepatic effects of glucagon in the eviscerated rat.", "content": "The in vivo effects of glucagon on the metabolism of extra-hepatic tissues have been investigated in eviscerated, functionally hepatectomized rats with intact kidneys. In these animals, even pharmacological amounts of exogenous glucagon did not significantly alter plasma glucose, FFA, or amino acids, compared with saline treatment. The possible secondary release of adrenal catecholamines following such doses of glucagon appeared to be similarly ineffective in increasing the peripheral tissue mobilization of substrates. It was only when the eviscerated animals were pretreated with insulin that the subsequent administration of glucagon or epinephrine elicited significant elevations in plasma FFA. The concomitant evisceration and adrenalectomy did not produce results which were significantly different from evisceration alone. Both kinds of animals required insulin pretreatment before a lipolytic response to glucagon or epinephrine could be demonstrated. This suggests that severe insulin insufficiency itself elicits almost maximum catabolism in these animals and that the further addition of other catabolic hormones such as glucagon or epinephrine cannot increase these catabolic effects, as manifest in plasma concentrations of FFA. These data show an extra-hepatic lipolytic effect of glucagon in vivo, but do not illuminate the significance of this effect in the intact animal.", "contents": "Studies on the extra-hepatic effects of glucagon in the eviscerated rat. The in vivo effects of glucagon on the metabolism of extra-hepatic tissues have been investigated in eviscerated, functionally hepatectomized rats with intact kidneys. In these animals, even pharmacological amounts of exogenous glucagon did not significantly alter plasma glucose, FFA, or amino acids, compared with saline treatment. The possible secondary release of adrenal catecholamines following such doses of glucagon appeared to be similarly ineffective in increasing the peripheral tissue mobilization of substrates. It was only when the eviscerated animals were pretreated with insulin that the subsequent administration of glucagon or epinephrine elicited significant elevations in plasma FFA. The concomitant evisceration and adrenalectomy did not produce results which were significantly different from evisceration alone. Both kinds of animals required insulin pretreatment before a lipolytic response to glucagon or epinephrine could be demonstrated. This suggests that severe insulin insufficiency itself elicits almost maximum catabolism in these animals and that the further addition of other catabolic hormones such as glucagon or epinephrine cannot increase these catabolic effects, as manifest in plasma concentrations of FFA. These data show an extra-hepatic lipolytic effect of glucagon in vivo, but do not illuminate the significance of this effect in the intact animal.", "PMID": 954659}
{"id": "pubmed23n0032_1237", "title": "Presence of melatonin in plasma and urine or pinealectomized rats.", "content": "Melatonin was shown to occur in rat plasma and urine after pinealectomy by bioassay, radioimmunoassay, and thin-layer chromatography. Total daily excretion of melatonin in pinealectomized rats was about 20% of control. Light-dark rhythms of plasma melatonin levels and urinary excretion rates disappeared after pinealectomy.", "contents": "Presence of melatonin in plasma and urine or pinealectomized rats. Melatonin was shown to occur in rat plasma and urine after pinealectomy by bioassay, radioimmunoassay, and thin-layer chromatography. Total daily excretion of melatonin in pinealectomized rats was about 20% of control. Light-dark rhythms of plasma melatonin levels and urinary excretion rates disappeared after pinealectomy.", "PMID": 954660}
{"id": "pubmed23n0032_1238", "title": "Catecholamine involvement in episodic luteinizing hormone release in adult ovariectomized rats.", "content": "This study was intended to examine the role of hypothalamic norepinephrine (HNE) and dopamine (HDA) in episodic luteinizing hormone (LH) release in adult ovariectomized rats. Unrestrained, unanesthetized rats with indwelling right atrial cannulae were bled continuously (30, 50, or 100 mul of whole blood/4-6 min for 3-4 hours), and the blood samples were analyzed for LH by radio-immunoassay. In other individual rats, changes in the hypothalamic levels of norepinephrine and dopamine after drug administration were determined by a radioisotopic-enzymatic catechol-O-methyl transferase assay. alpha-Methyl-p-tyrosine significantly decreased HNE and HDA concentrations but failed to alter episodic LH release. Two dopamine-beta-hydroxylase inhibitors (U-14,624 and FLA-63) caused marked reductions in HNE, small but not statistically significant increases in HDA, and an inhibition of episodic LH secretion. Apomorphine, a dopamine receptor stimulator, caused a transient (50-60 min) but marked inhibition of episodic LH release. Saline injection had no effect. Pimozide, a blocker of dopamine receptors, prevented the inhibitory effects seen following apomorphine. Although not studied in detail, pimozide alone did not appear to alter episodic LH secretion. These data suggest that in adult ovariectomized rats norepinephrine may be an excitatory neurotransmitter in the modulation of episodic LH release. The activation of dopamine receptors may be capable of inhibiting this release process. However, the apparent inability of pimozide alone to alter episodic LH discharge suggests that under physiological conditions dopamine may not play a role in the modulation of episodic LH secretion.", "contents": "Catecholamine involvement in episodic luteinizing hormone release in adult ovariectomized rats. This study was intended to examine the role of hypothalamic norepinephrine (HNE) and dopamine (HDA) in episodic luteinizing hormone (LH) release in adult ovariectomized rats. Unrestrained, unanesthetized rats with indwelling right atrial cannulae were bled continuously (30, 50, or 100 mul of whole blood/4-6 min for 3-4 hours), and the blood samples were analyzed for LH by radio-immunoassay. In other individual rats, changes in the hypothalamic levels of norepinephrine and dopamine after drug administration were determined by a radioisotopic-enzymatic catechol-O-methyl transferase assay. alpha-Methyl-p-tyrosine significantly decreased HNE and HDA concentrations but failed to alter episodic LH release. Two dopamine-beta-hydroxylase inhibitors (U-14,624 and FLA-63) caused marked reductions in HNE, small but not statistically significant increases in HDA, and an inhibition of episodic LH secretion. Apomorphine, a dopamine receptor stimulator, caused a transient (50-60 min) but marked inhibition of episodic LH release. Saline injection had no effect. Pimozide, a blocker of dopamine receptors, prevented the inhibitory effects seen following apomorphine. Although not studied in detail, pimozide alone did not appear to alter episodic LH secretion. These data suggest that in adult ovariectomized rats norepinephrine may be an excitatory neurotransmitter in the modulation of episodic LH release. The activation of dopamine receptors may be capable of inhibiting this release process. However, the apparent inability of pimozide alone to alter episodic LH discharge suggests that under physiological conditions dopamine may not play a role in the modulation of episodic LH secretion.", "PMID": 954661}
{"id": "pubmed23n0032_1239", "title": "Electrical stimulation of the arcuate nucleus in ovariectomized rats inhibits episodic luteinizing hormone (LH) release but excites LH release after estrogen priming.", "content": "The effect of electrical stimulation of the arcuate nucleus of the hypothalamus (ARH) on the blood levels of luteinizing hormone (LH) was studied in ovariectomized (ovx) rats. Unanesthetized, unrestrained rats were bled continuously (30 or 50 mul whole blood/4-6 min) through indwelling right atrial cannulae for 1 1/2-2h prior to the onset of stimulation. The bleeding was continued for the following three hours, during which electrical stimulation was applied for two 60 min periods separated by a 60 min non-stimulation period. Whole blood was analyzed for LH by radioimmunoassay. Electrical stimulation of the ARH in ovx rats inhibited the episodic LH release characteristic of these animals. The inhibition was rapid in onset, beginning within minutes, and lasted for most if not all of the stimulation period or longer. The blood LH levels returned to control values prior to the second stimulation period during which the inhibitory response was again seen. Stimulation in other hypothalamic areas outside the ARH was generally ineffective, although repeatable inhibition of episodic LH release did occur in 2 of 3 rats stimulated in the periventricular nucleus. In a second study, ovx rats were primed with 5 mug estradiol benzoate/100 g body weight/day for 2 days prior to stimulation. In these rats, stimulation of the ARH or median eminence produced increases in LH release, whereas stimulation in other hypothalamic areas outside the ARH was ineffective. The parameters of electrical stimulation that produced increases in LH release were the same as those that caused the inhibition of episodic LH release. The results demonstrate that electrical stimulation of the ARH inhibits LH release in ovx rats, but increases LH release if the animals have been primed with estrogen.", "contents": "Electrical stimulation of the arcuate nucleus in ovariectomized rats inhibits episodic luteinizing hormone (LH) release but excites LH release after estrogen priming. The effect of electrical stimulation of the arcuate nucleus of the hypothalamus (ARH) on the blood levels of luteinizing hormone (LH) was studied in ovariectomized (ovx) rats. Unanesthetized, unrestrained rats were bled continuously (30 or 50 mul whole blood/4-6 min) through indwelling right atrial cannulae for 1 1/2-2h prior to the onset of stimulation. The bleeding was continued for the following three hours, during which electrical stimulation was applied for two 60 min periods separated by a 60 min non-stimulation period. Whole blood was analyzed for LH by radioimmunoassay. Electrical stimulation of the ARH in ovx rats inhibited the episodic LH release characteristic of these animals. The inhibition was rapid in onset, beginning within minutes, and lasted for most if not all of the stimulation period or longer. The blood LH levels returned to control values prior to the second stimulation period during which the inhibitory response was again seen. Stimulation in other hypothalamic areas outside the ARH was generally ineffective, although repeatable inhibition of episodic LH release did occur in 2 of 3 rats stimulated in the periventricular nucleus. In a second study, ovx rats were primed with 5 mug estradiol benzoate/100 g body weight/day for 2 days prior to stimulation. In these rats, stimulation of the ARH or median eminence produced increases in LH release, whereas stimulation in other hypothalamic areas outside the ARH was ineffective. The parameters of electrical stimulation that produced increases in LH release were the same as those that caused the inhibition of episodic LH release. The results demonstrate that electrical stimulation of the ARH inhibits LH release in ovx rats, but increases LH release if the animals have been primed with estrogen.", "PMID": 954662}
{"id": "pubmed23n0032_1240", "title": "Direct stimulation by growth hormone of glucagon and insulin release from isolated rat pancreas.", "content": "The acute effects of bovine growth hormone (bGH) upon the release of glucagon and insulin from the isolated perfused rat pancreas were investigated in the presence of 5.6 mM glucose. Glucagon and insulin release occurred in response to 10(-9), 10(-8) and 10(-7)M bGH within 1-2 min and dissipated within 4 min; a significant increase was seen at 24 sec for glucagon and at 48 sec for insulin. In this concentration range, a dose-response relationship was established for both islet hormones. Upon the addition of 5 mM fumarate, glutamate, and pyruvate, glucagon and insulin release occurred also in response to 10(-11) and 10(-10)M bGH. In the absence of glucose, in response to 10(-7)M bGH, the magnitude of glucagon release was similar to that seen with 5.6 mM glucose; but the release of insulin failed to occur. In the presence of 16.7 mM glucose, the release of glucagon was blunted and that of insulin augmented. The data indicate that growth hormone can stimulate acutely the release of glucagon and insulin at physiological as well as pharmacological concentrations. These rapid and short-lived effects are likely to be direct and not mediated through the generation of somatomedin. The stimulatory effect of growth hormone appears to favor the release of glucagon under conditions of glucose lack, and of insulin under conditions of glucose abundance. These results indicate that growth hormone may have a tonic effect on islet hormone release.", "contents": "Direct stimulation by growth hormone of glucagon and insulin release from isolated rat pancreas. The acute effects of bovine growth hormone (bGH) upon the release of glucagon and insulin from the isolated perfused rat pancreas were investigated in the presence of 5.6 mM glucose. Glucagon and insulin release occurred in response to 10(-9), 10(-8) and 10(-7)M bGH within 1-2 min and dissipated within 4 min; a significant increase was seen at 24 sec for glucagon and at 48 sec for insulin. In this concentration range, a dose-response relationship was established for both islet hormones. Upon the addition of 5 mM fumarate, glutamate, and pyruvate, glucagon and insulin release occurred also in response to 10(-11) and 10(-10)M bGH. In the absence of glucose, in response to 10(-7)M bGH, the magnitude of glucagon release was similar to that seen with 5.6 mM glucose; but the release of insulin failed to occur. In the presence of 16.7 mM glucose, the release of glucagon was blunted and that of insulin augmented. The data indicate that growth hormone can stimulate acutely the release of glucagon and insulin at physiological as well as pharmacological concentrations. These rapid and short-lived effects are likely to be direct and not mediated through the generation of somatomedin. The stimulatory effect of growth hormone appears to favor the release of glucagon under conditions of glucose lack, and of insulin under conditions of glucose abundance. These results indicate that growth hormone may have a tonic effect on islet hormone release.", "PMID": 954663}
{"id": "pubmed23n0032_1241", "title": "Influence of synthetic somatostatin upon growth hormone release from perifused rat pituitaries.", "content": "Basal release of pre-labeled, stored tritiated rat growth hormone ([3H]rGH) from perfused rat pituitary explants is a constant fraction of pituitary [LH]rGH content. Synthetic somatostatin (SRIF) inhibits the release of pre-labeled, stored [3H]rGH in a dose-dependent fashion. Prolonged exposure to SRIF results in an immediate and continuous inhibition of [3H]rGH release. In the in vitro perifusion system, the maximal inhibition, which is achieved with 25 nM SRIF, results in a rate of [3H]rGH release which is 30 to 40 per cent of basal release. Pulses of SRIF produce inhibition of [3H]rGH release followed by rebound release after withdrawal of SRIF. When the time of exposure to SRIF is held constant, both the SRIF-induced inhibition and the rebound release of [3H]rGH are dose-dependent. A similar progressive response is seen when a constant SRIF concentration is pulsed for variable periods of time. There is no net inhibition of [3H]rGH release by pulses of SRIF.", "contents": "Influence of synthetic somatostatin upon growth hormone release from perifused rat pituitaries. Basal release of pre-labeled, stored tritiated rat growth hormone ([3H]rGH) from perfused rat pituitary explants is a constant fraction of pituitary [LH]rGH content. Synthetic somatostatin (SRIF) inhibits the release of pre-labeled, stored [3H]rGH in a dose-dependent fashion. Prolonged exposure to SRIF results in an immediate and continuous inhibition of [3H]rGH release. In the in vitro perifusion system, the maximal inhibition, which is achieved with 25 nM SRIF, results in a rate of [3H]rGH release which is 30 to 40 per cent of basal release. Pulses of SRIF produce inhibition of [3H]rGH release followed by rebound release after withdrawal of SRIF. When the time of exposure to SRIF is held constant, both the SRIF-induced inhibition and the rebound release of [3H]rGH are dose-dependent. A similar progressive response is seen when a constant SRIF concentration is pulsed for variable periods of time. There is no net inhibition of [3H]rGH release by pulses of SRIF.", "PMID": 954664}
{"id": "pubmed23n0032_1242", "title": "Ultradian growth hormone rhythm in the rat: effects of feeding, hyperglycemia, and insulin-induced hypoglycemia.", "content": "Temporal patterns of plasma GH, immunoreactive insulin (IRI), and glucose were defined by obtaining serial blood samples from freely-moving male rats bearing chronic intracardiac venous cannulae.  Blood was withdrawn every 15 min for periods of 6 h.  Plasma GH and IRI were determined by radioimmunoassay.  The typical ultradian rhythm of GH secretion was evident in each undisturbed animal (peaks greater than 200 ng/ml; troughs less than 1 ng/ml; mean period:  3.40 +/-0.08 h).  Basal plasma IRI and glucose levels fluctuated minimally.  There was no significant correlation between plasma GH and IRI, GH and glucose, or IRI and glucose levels in unfed rats.  The rhythmic GH secretory patterns of feeding animals (mean period: 3.12 +/-0.16 h; peaks greater than 200 ng/ml; troughs less than 1 ng/ml) were similar to those of non-feeding animals (mean period:  3.34 +/-0.15 h; peaks greater than 200 ng/ml; troughs less than 1 ng/ml) despite large fluctuations in plasma IRI levels and a wide variation in the number and size of the meals taken.  No consistent relation was observed between the ingestion of meals and the bursts of GH secretion.  The mean period of the GH rhythm was not significantly altered by hyperglycemia (mean period; 3.25 +/- 0.08 h), although the amplitude of the pulses of half of the hyperglycemic rats was markedly depressed. Insulin-induced hypoglycemia caused a significant depression in the amplitude of the GH pulses; however, the pattern of this response was not consistent.  Despite wide variability in the GH response, the magnitude and time course of recovery of the plasma glucose levels was similar in all animals. These results suggest that GH secretion in the rat is regulated primarily by an endogenous ultradian rhythm which is not dependent on changes in plasma glucose or IRI levels, and continues to function independently of feeding behavior.  It is unlikely that GH is an important physiologic regulator of glucose homeostasis in this species.", "contents": "Ultradian growth hormone rhythm in the rat: effects of feeding, hyperglycemia, and insulin-induced hypoglycemia. Temporal patterns of plasma GH, immunoreactive insulin (IRI), and glucose were defined by obtaining serial blood samples from freely-moving male rats bearing chronic intracardiac venous cannulae.  Blood was withdrawn every 15 min for periods of 6 h.  Plasma GH and IRI were determined by radioimmunoassay.  The typical ultradian rhythm of GH secretion was evident in each undisturbed animal (peaks greater than 200 ng/ml; troughs less than 1 ng/ml; mean period:  3.40 +/-0.08 h).  Basal plasma IRI and glucose levels fluctuated minimally.  There was no significant correlation between plasma GH and IRI, GH and glucose, or IRI and glucose levels in unfed rats.  The rhythmic GH secretory patterns of feeding animals (mean period: 3.12 +/-0.16 h; peaks greater than 200 ng/ml; troughs less than 1 ng/ml) were similar to those of non-feeding animals (mean period:  3.34 +/-0.15 h; peaks greater than 200 ng/ml; troughs less than 1 ng/ml) despite large fluctuations in plasma IRI levels and a wide variation in the number and size of the meals taken.  No consistent relation was observed between the ingestion of meals and the bursts of GH secretion.  The mean period of the GH rhythm was not significantly altered by hyperglycemia (mean period; 3.25 +/- 0.08 h), although the amplitude of the pulses of half of the hyperglycemic rats was markedly depressed. Insulin-induced hypoglycemia caused a significant depression in the amplitude of the GH pulses; however, the pattern of this response was not consistent.  Despite wide variability in the GH response, the magnitude and time course of recovery of the plasma glucose levels was similar in all animals. These results suggest that GH secretion in the rat is regulated primarily by an endogenous ultradian rhythm which is not dependent on changes in plasma glucose or IRI levels, and continues to function independently of feeding behavior.  It is unlikely that GH is an important physiologic regulator of glucose homeostasis in this species.", "PMID": 954665}
{"id": "pubmed23n0032_1243", "title": "Negative feedback control of LH by testosterone: a quantitative study in male rats.", "content": "Adult male rats were castrated and implanted sc with testosterone (T)-filled Silastic capsules of different sizes which produce stable plasma T levels proportional to capsule length.  T and LH levels were determined in blood samples obtained at 3-day intervals for 21 days.  Plasma T values greater than 1.8 ng/ml suppressed plasma LH to levels below the intact range, while a plasma T of less than 0.5 ng/ml yeilded no significant inhibition of the post-castration LH rise.  Animals with a plasma T of about 0.7 ng/ml, however, showed intact, castrate, or immediate LH levels.  The feedback-effective range for all animals was between 0.5 ngT/mg (castrate-like LH response) and 1.8 ng/ml (LH at or below intact levels), but individual rats seemed to have a more restricted feedback range than that indicated by these group data.  To test this hypothesis further, rats were implanted at the time of castration with five 5-mm T-filled capsules.  One capsule was removed each week, producing step function drops in plasma T, and allowing examination of the LH responses of individual animals.  Plasma t levels greater than 1.6 ng/ml maintained LH values at or below intact levels.  However, when plasma T dropped to 1.1 ng/ml, some rats showed castration-like LH increase and others did not respond until after a further drop to 0.6 ng/ml.  It was concluded that the feedback-effective range of plasma T is very restricted and differs in individual rats.", "contents": "Negative feedback control of LH by testosterone: a quantitative study in male rats. Adult male rats were castrated and implanted sc with testosterone (T)-filled Silastic capsules of different sizes which produce stable plasma T levels proportional to capsule length.  T and LH levels were determined in blood samples obtained at 3-day intervals for 21 days.  Plasma T values greater than 1.8 ng/ml suppressed plasma LH to levels below the intact range, while a plasma T of less than 0.5 ng/ml yeilded no significant inhibition of the post-castration LH rise.  Animals with a plasma T of about 0.7 ng/ml, however, showed intact, castrate, or immediate LH levels.  The feedback-effective range for all animals was between 0.5 ngT/mg (castrate-like LH response) and 1.8 ng/ml (LH at or below intact levels), but individual rats seemed to have a more restricted feedback range than that indicated by these group data.  To test this hypothesis further, rats were implanted at the time of castration with five 5-mm T-filled capsules.  One capsule was removed each week, producing step function drops in plasma T, and allowing examination of the LH responses of individual animals.  Plasma t levels greater than 1.6 ng/ml maintained LH values at or below intact levels.  However, when plasma T dropped to 1.1 ng/ml, some rats showed castration-like LH increase and others did not respond until after a further drop to 0.6 ng/ml.  It was concluded that the feedback-effective range of plasma T is very restricted and differs in individual rats.", "PMID": 954666}
{"id": "pubmed23n0032_1244", "title": "Seasonal profiles of plasma luteinizing hormone, testosterone and estradiol in the ram.", "content": "Plasma profiles of luteinizing hormone (LH), testosterone (T), and estradiol (E2) have been determined in five mature rams during the primary breeding season (September) and again when breeding activity was low (May). Blood samples collected every half h for 24 h during September or May showed that LH was released into the general circulation as distinct peaks. Although this secretory pattern was not the same for all rams and seasonal differences were noted in certain individuals, it is suggested that an inherent rhythm persists within each ram. With the exception of one ram in September, obvious T peaks were preceded by LH peaks greater than 2 ng/ml (LH-T interval less than 60 min). Season had no effect on the number and magnitude of LH peaks or on the mean concentrations; however, basal levels of LH were higher (P less than 0.01) in September than in May. Seasonal differences in peripheral T levels were more dramatic with mean, baseline, and peak concentrations elevated (p less than 0.01) during the fall. The number of T peaks, like those of LH, was not affected by season. Large variations in circulating levels of E2 made any possible relationship between this hormone and LH or T difficult to identify. Furthermore, a diurnal or seasonal rhythm could not be established for E2 in this study. It is concluded that seasonal differences occur in secretory patterns of LH and T and that a cause and effect relationship between these two hormones exists. Under conditions of this study, neither a consistent relationship between E2 and LH or T, nor a seasonal difference in mean E2 levels, was apparent.", "contents": "Seasonal profiles of plasma luteinizing hormone, testosterone and estradiol in the ram. Plasma profiles of luteinizing hormone (LH), testosterone (T), and estradiol (E2) have been determined in five mature rams during the primary breeding season (September) and again when breeding activity was low (May). Blood samples collected every half h for 24 h during September or May showed that LH was released into the general circulation as distinct peaks. Although this secretory pattern was not the same for all rams and seasonal differences were noted in certain individuals, it is suggested that an inherent rhythm persists within each ram. With the exception of one ram in September, obvious T peaks were preceded by LH peaks greater than 2 ng/ml (LH-T interval less than 60 min). Season had no effect on the number and magnitude of LH peaks or on the mean concentrations; however, basal levels of LH were higher (P less than 0.01) in September than in May. Seasonal differences in peripheral T levels were more dramatic with mean, baseline, and peak concentrations elevated (p less than 0.01) during the fall. The number of T peaks, like those of LH, was not affected by season. Large variations in circulating levels of E2 made any possible relationship between this hormone and LH or T difficult to identify. Furthermore, a diurnal or seasonal rhythm could not be established for E2 in this study. It is concluded that seasonal differences occur in secretory patterns of LH and T and that a cause and effect relationship between these two hormones exists. Under conditions of this study, neither a consistent relationship between E2 and LH or T, nor a seasonal difference in mean E2 levels, was apparent.", "PMID": 954667}
{"id": "pubmed23n0032_1245", "title": "Evidence for adrenergic mediation of cholinergic inhibition of prolactin release.", "content": "Pilocarpine, a cholinergic agonist, significantly reduced the high levels of serum prolactin in estrogen-primed male rats and in female rats on the late afternoon of proestrus. In male rats treated with reserpine, chlorpromazine, haloperidol, or pimozide, serum prolactin levels were greatly elevated. Subsequent treatment with pilocarpine failed to reduce serum prolactin concentrations in these rats. When atropine, a cholinergic antagonist, was injected ip in doses of 3 to 250 mg/kg into male rats, prolactin release was not altered. However, when atropine was injected prior to pilocarpine, it prevented the reduction in serum prolactin by the latter drug. Methyl-atropine, which does not enter the CNS, did not prevent pilocarpine from inhibiting prolactin release. These results suggest that cholinergic inhibition of prolactin release is mediated via adrenergic neurons, and thus support a role for a cholinergic link in hypothalamic regulation of prolactin release.", "contents": "Evidence for adrenergic mediation of cholinergic inhibition of prolactin release. Pilocarpine, a cholinergic agonist, significantly reduced the high levels of serum prolactin in estrogen-primed male rats and in female rats on the late afternoon of proestrus. In male rats treated with reserpine, chlorpromazine, haloperidol, or pimozide, serum prolactin levels were greatly elevated. Subsequent treatment with pilocarpine failed to reduce serum prolactin concentrations in these rats. When atropine, a cholinergic antagonist, was injected ip in doses of 3 to 250 mg/kg into male rats, prolactin release was not altered. However, when atropine was injected prior to pilocarpine, it prevented the reduction in serum prolactin by the latter drug. Methyl-atropine, which does not enter the CNS, did not prevent pilocarpine from inhibiting prolactin release. These results suggest that cholinergic inhibition of prolactin release is mediated via adrenergic neurons, and thus support a role for a cholinergic link in hypothalamic regulation of prolactin release.", "PMID": 954668}
{"id": "pubmed23n0032_1246", "title": "The insulinotropic action of gastric inhibitory polypeptide in the perfused isolated rat pancreas.", "content": "Gastric inhibitory polypeptide (GIP) produced a dose-related increase in immunoreactive insulin (IRI) from the perfused isolated rat pancreas. The doses employed were within physiological limits. This effect was glucose-concentration-dependent in that there existed a threshold concentration of glucose above which GIP exerted the insulinotropic action, and that, at a fixed concentration of GIP, increased glucose concentrations stimulated IRI release in more than an additive manner. A biologically active fragment of the GIP molecule was isolated and purified. All criteria have been satisfied that GIP is an insulinotropic hormone.", "contents": "The insulinotropic action of gastric inhibitory polypeptide in the perfused isolated rat pancreas. Gastric inhibitory polypeptide (GIP) produced a dose-related increase in immunoreactive insulin (IRI) from the perfused isolated rat pancreas. The doses employed were within physiological limits. This effect was glucose-concentration-dependent in that there existed a threshold concentration of glucose above which GIP exerted the insulinotropic action, and that, at a fixed concentration of GIP, increased glucose concentrations stimulated IRI release in more than an additive manner. A biologically active fragment of the GIP molecule was isolated and purified. All criteria have been satisfied that GIP is an insulinotropic hormone.", "PMID": 954669}
{"id": "pubmed23n0032_1247", "title": "Gonadotrophin stimulation of progesterone synthesis by midpregnancy mouse ovarian cells in vitro.", "content": "The ability of cultured midpregnancy mouse ovarian cells to synthesize progesterone de novo and from oxogenous pregnenolone has been assessed. The conversion of pregnenolone to progesterone is almost completely blocked by cyanoketone, a known inhibitor of delta5,3beta-hydroxysteroid dehydrogenase (3beta-HSD) activity, but is unaffected by aminoglutethimide, which inhibits the cholesterol side-chain cleavage enzyme complex (desmolase). Since there is little metabolism of the formed progesterone, the ability of ovarian cells to convert exogenous pregnenolone to progesterone in vitro reflects the activity of 3beta-HSD in these cells. Cultured ovarian cells are also capable of endogenous progesterone production in the absence of added pregnenolone, although the absolute amount of progesterone produced is considerably less than that produced from exogenous pregnenolone. Since endogenous progesterone accumulation is almost completely blocked by the addition of aminoglutethimide to the culture medium, it is concluded that this response does represent de novo progesterone synthesis. Neither bovine luteinizing hormone (LH) nor human chorionic gonadotrophin (hCG) affects 3beta-HSD activity in cultured ovarian cells. The ability of the cells to secrete or to further metabolize the progesterone formed is also unaffected. However, both LH and hCG stimulate endogenous progesterone production within one hour of their addition to the culture medium. The stimulation, 2-10 fold in several experiments, can be maintained for at least six days of culture, and is not a result of an increase in the growth rate of the ovarian cells. As would be expected, the stimulation is blocked by the addition of aminoglutethimide to the culture medium. Taken together, these facts suggest that gonadotrophic hormones stimulate progesterone production by ovarian cells specifically by their action at steps prior to the conversion of pregnenolone to progesterone.", "contents": "Gonadotrophin stimulation of progesterone synthesis by midpregnancy mouse ovarian cells in vitro. The ability of cultured midpregnancy mouse ovarian cells to synthesize progesterone de novo and from oxogenous pregnenolone has been assessed. The conversion of pregnenolone to progesterone is almost completely blocked by cyanoketone, a known inhibitor of delta5,3beta-hydroxysteroid dehydrogenase (3beta-HSD) activity, but is unaffected by aminoglutethimide, which inhibits the cholesterol side-chain cleavage enzyme complex (desmolase). Since there is little metabolism of the formed progesterone, the ability of ovarian cells to convert exogenous pregnenolone to progesterone in vitro reflects the activity of 3beta-HSD in these cells. Cultured ovarian cells are also capable of endogenous progesterone production in the absence of added pregnenolone, although the absolute amount of progesterone produced is considerably less than that produced from exogenous pregnenolone. Since endogenous progesterone accumulation is almost completely blocked by the addition of aminoglutethimide to the culture medium, it is concluded that this response does represent de novo progesterone synthesis. Neither bovine luteinizing hormone (LH) nor human chorionic gonadotrophin (hCG) affects 3beta-HSD activity in cultured ovarian cells. The ability of the cells to secrete or to further metabolize the progesterone formed is also unaffected. However, both LH and hCG stimulate endogenous progesterone production within one hour of their addition to the culture medium. The stimulation, 2-10 fold in several experiments, can be maintained for at least six days of culture, and is not a result of an increase in the growth rate of the ovarian cells. As would be expected, the stimulation is blocked by the addition of aminoglutethimide to the culture medium. Taken together, these facts suggest that gonadotrophic hormones stimulate progesterone production by ovarian cells specifically by their action at steps prior to the conversion of pregnenolone to progesterone.", "PMID": 954670}
{"id": "pubmed23n0032_1248", "title": "Pressor action of centrally perfused angiotensin II in rats with hereditary hypothalamic diabetes insipidus.", "content": "A method was devised for the perfusion of the cerebral ventricles in conscious rats. Using this method a basal secretion rate of 15 +/- 3 pg of immunoreactive angiotensin II per min was calculated. This material was suggested to be of extrarenal origin. In comparison to findings in normal Long-Evans rats, pressor responses to intraventricular perfusions of angiotensin II were reduced in rats heterozygous for hypothalamic diabetes insipidus and virtually absent in rats homozygous for the hypothalamic deficiency whether they were treated with vasopressin or not. The pressor response to intraventricular angiotensin II is suggested to be related to the release of vasopressin.", "contents": "Pressor action of centrally perfused angiotensin II in rats with hereditary hypothalamic diabetes insipidus. A method was devised for the perfusion of the cerebral ventricles in conscious rats. Using this method a basal secretion rate of 15 +/- 3 pg of immunoreactive angiotensin II per min was calculated. This material was suggested to be of extrarenal origin. In comparison to findings in normal Long-Evans rats, pressor responses to intraventricular perfusions of angiotensin II were reduced in rats heterozygous for hypothalamic diabetes insipidus and virtually absent in rats homozygous for the hypothalamic deficiency whether they were treated with vasopressin or not. The pressor response to intraventricular angiotensin II is suggested to be related to the release of vasopressin.", "PMID": 954671}
{"id": "pubmed23n0032_1249", "title": "Maturation and survival of spermatozoa in the epididymis of pregnenolone treated hypophysectomized rats.", "content": "Adult hypophysectomized male rats were injected daily with 2 mg pregnenolone for 30 days. In these rats the testosterone concentrations in peripheral blood (0.5 ng/ml) and testes (46 ng/g) were appreciably lower than in intact rats (4.1 ng/ml and 167 ng/g, respectively). In hypophysectomized animals treated with pregnenolone the testes and epididymides were much better maintained than the prostates and seminal vesicles. High concentrations of dihydrotestosterone were found to be present in the head (12 ng/g) and body (8 ng/g) of the epididymis of these rats. Although the number of spermatozoa in the distal part of the epididymis of pregnenolone-treated hypophysectomized rats was only 23% of the number found in intact control animals, the spermatozoa were fertile.", "contents": "Maturation and survival of spermatozoa in the epididymis of pregnenolone treated hypophysectomized rats. Adult hypophysectomized male rats were injected daily with 2 mg pregnenolone for 30 days. In these rats the testosterone concentrations in peripheral blood (0.5 ng/ml) and testes (46 ng/g) were appreciably lower than in intact rats (4.1 ng/ml and 167 ng/g, respectively). In hypophysectomized animals treated with pregnenolone the testes and epididymides were much better maintained than the prostates and seminal vesicles. High concentrations of dihydrotestosterone were found to be present in the head (12 ng/g) and body (8 ng/g) of the epididymis of these rats. Although the number of spermatozoa in the distal part of the epididymis of pregnenolone-treated hypophysectomized rats was only 23% of the number found in intact control animals, the spermatozoa were fertile.", "PMID": 954672}
{"id": "pubmed23n0032_1250", "title": "Sympathetic nerve endings in the pineal gland protect against acute stress-induced increase in N-acetyltransferase (EC 2.3.1.5.) activity.", "content": "Injection of the antidepressant desmyethylimipramine (DMI, 10 mg/kg) into intact rats or into rats in which the superior cervical ganglia had been decentralized caused a marked enhancement of the swimming stress-induced increase in pineal gland acetyl-CoA:serotonin N-acetyltransferase (N-acetyltransferase, EC 2.3.1.5) activity. DMI is known to block uptake, the transport of catecholamines by nerve endings. It was found that DMI had no effect on enzyme activity in superior cervical ganglionectomized (SCGX) rats which were swimming stressed. The pineal glands of these animals are devoid of nerve endings. In unstressed intact or unstressed surgically altered rats, injection of DMI caused only a minor increase in N-acetyltransferase activity, which was much smaller than that seen after stress. After 5 h in organ culture sympathetic nerve endings within the pineal gland are still intact. At this time DMI treatment of pineal glands taken from intact rats shifted the dose-response curve for epinephrine (EPI) stimulation of N-acetyltransferase activity by two orders of magnitude, but caused only a slight change in the dose-response curve for isoproterenol, which is not taken up into nerve endings. In contrast, DMI treatment in organ culture had no effect on the dose-response curve for EPI in denervated pineal glands. These results support the hypothesis that the response of pineal N-acetyltransferase activity to stimulation by stress is influenced by uptake. It would appear that in addition to terminating neuronal adrenergic transmission, this transport process physiologically protects the pineal gland against nontranssynaptic adrenergic stimulation.", "contents": "Sympathetic nerve endings in the pineal gland protect against acute stress-induced increase in N-acetyltransferase (EC 2.3.1.5.) activity. Injection of the antidepressant desmyethylimipramine (DMI, 10 mg/kg) into intact rats or into rats in which the superior cervical ganglia had been decentralized caused a marked enhancement of the swimming stress-induced increase in pineal gland acetyl-CoA:serotonin N-acetyltransferase (N-acetyltransferase, EC 2.3.1.5) activity. DMI is known to block uptake, the transport of catecholamines by nerve endings. It was found that DMI had no effect on enzyme activity in superior cervical ganglionectomized (SCGX) rats which were swimming stressed. The pineal glands of these animals are devoid of nerve endings. In unstressed intact or unstressed surgically altered rats, injection of DMI caused only a minor increase in N-acetyltransferase activity, which was much smaller than that seen after stress. After 5 h in organ culture sympathetic nerve endings within the pineal gland are still intact. At this time DMI treatment of pineal glands taken from intact rats shifted the dose-response curve for epinephrine (EPI) stimulation of N-acetyltransferase activity by two orders of magnitude, but caused only a slight change in the dose-response curve for isoproterenol, which is not taken up into nerve endings. In contrast, DMI treatment in organ culture had no effect on the dose-response curve for EPI in denervated pineal glands. These results support the hypothesis that the response of pineal N-acetyltransferase activity to stimulation by stress is influenced by uptake. It would appear that in addition to terminating neuronal adrenergic transmission, this transport process physiologically protects the pineal gland against nontranssynaptic adrenergic stimulation.", "PMID": 954673}
{"id": "pubmed23n0032_1251", "title": "Parathyroid hormone as a possible causal factor in osteopetrosis of the tl rat.", "content": "The possibility of parathyroid dysfunction as a causal factor in the osteopetrosis of the tl rats was explored by evaluating serum calcium (Ca) and immunoreactive parathyroid hormone (iPTH) concentrations in this strain of rats, as compared with those of phenotypically normal littermate (LM) and non-littermate (NLM) control groups. The mean serum Ca concentration in the tl rat was not significantly different from that of the NLM group, although it was less than that of the LM group. However, all Ca values were within the normal range. The mean serum iPTH concentration in the tl rats was not significantly different from those of either control group. The data indicate that the adult tl rat has adequate but not excessive PTH secretion. Therefore, osteopetrosis and its manifestations in the adult tl are not caused by an abnormality of parathyroid function.", "contents": "Parathyroid hormone as a possible causal factor in osteopetrosis of the tl rat. The possibility of parathyroid dysfunction as a causal factor in the osteopetrosis of the tl rats was explored by evaluating serum calcium (Ca) and immunoreactive parathyroid hormone (iPTH) concentrations in this strain of rats, as compared with those of phenotypically normal littermate (LM) and non-littermate (NLM) control groups. The mean serum Ca concentration in the tl rat was not significantly different from that of the NLM group, although it was less than that of the LM group. However, all Ca values were within the normal range. The mean serum iPTH concentration in the tl rats was not significantly different from those of either control group. The data indicate that the adult tl rat has adequate but not excessive PTH secretion. Therefore, osteopetrosis and its manifestations in the adult tl are not caused by an abnormality of parathyroid function.", "PMID": 954674}
{"id": "pubmed23n0032_1252", "title": "Anesthetics and thyrotropin secretion in the rat.", "content": "The effects of seven anesthetics (thiopentone, 50 mg/kg ip; pentobarbitone, 50 mg/kg ip; chloral hydrate, 300 mg/kg ip; urethane, 1,5 g/kg, 1/2ip, 1/2sc; ether; methoxyflurane, 1,5%; halothane, 2%) on basal serum TSH concentrations and on the cold-induced as well as the TRH-induced TSH responses were studied in Sprague-Dawley rats. The basal TSH level in female rats were decreased by ether and halothane at 30 min and somewhat increased by pentobarbitone and chloral hydrate. The cold-induced (4C, 30-60 min) TSH response of the warm-adapted male rats (30 C, 7 days) was decreased by all of the anesthetics studied, but the effect of pentobarbitone was not significant. The TRH-induced (50 ng iv) TSH response in female rats was totally abolished only by deep ether anesthesia but augmented by bariturates and chloral hydrate. It is concluded that all of the anesthetics studied can modify the secretion of TSH by their central effects. Ether in high concentration seems to be effective also at the pituitary level. The use of anesthetics may be a source of error when studying the neurotransmitter control of TSH-TRH secretion in the rat.", "contents": "Anesthetics and thyrotropin secretion in the rat. The effects of seven anesthetics (thiopentone, 50 mg/kg ip; pentobarbitone, 50 mg/kg ip; chloral hydrate, 300 mg/kg ip; urethane, 1,5 g/kg, 1/2ip, 1/2sc; ether; methoxyflurane, 1,5%; halothane, 2%) on basal serum TSH concentrations and on the cold-induced as well as the TRH-induced TSH responses were studied in Sprague-Dawley rats. The basal TSH level in female rats were decreased by ether and halothane at 30 min and somewhat increased by pentobarbitone and chloral hydrate. The cold-induced (4C, 30-60 min) TSH response of the warm-adapted male rats (30 C, 7 days) was decreased by all of the anesthetics studied, but the effect of pentobarbitone was not significant. The TRH-induced (50 ng iv) TSH response in female rats was totally abolished only by deep ether anesthesia but augmented by bariturates and chloral hydrate. It is concluded that all of the anesthetics studied can modify the secretion of TSH by their central effects. Ether in high concentration seems to be effective also at the pituitary level. The use of anesthetics may be a source of error when studying the neurotransmitter control of TSH-TRH secretion in the rat.", "PMID": 954675}
{"id": "pubmed23n0032_1253", "title": "Influence of superoxide dismutase and catalase on the stimulation by phagocytosis of L-thyroxine and L-triiodothyronine deiodination in the human leukocyte.", "content": "Neither superoxide dismutase, which destroys the superoxide radical by catalyzing its conversion to hydrogen peroxide, nor catalase prevented the stimulation by phagocytosis of the deiodination of L-thyroxine or L-triiodothyronine in human leukocytes. On the other hand, superoxide dismutase led to an increase in the proportion of iodinated material, which remained at the origin during chromatography in a butanol-acetic acid-water solvent system, whereas catalase had the opposite effect. The data suggest that neither the superoxide radical nor hydrogen peroxide plays a major role in the stimulation of deiodination of hormone, but that once deiodination has occurred, hydrogen peroxide is more important than that superoxide radical in subsequent iodination reactions within the leukocyte.", "contents": "Influence of superoxide dismutase and catalase on the stimulation by phagocytosis of L-thyroxine and L-triiodothyronine deiodination in the human leukocyte. Neither superoxide dismutase, which destroys the superoxide radical by catalyzing its conversion to hydrogen peroxide, nor catalase prevented the stimulation by phagocytosis of the deiodination of L-thyroxine or L-triiodothyronine in human leukocytes. On the other hand, superoxide dismutase led to an increase in the proportion of iodinated material, which remained at the origin during chromatography in a butanol-acetic acid-water solvent system, whereas catalase had the opposite effect. The data suggest that neither the superoxide radical nor hydrogen peroxide plays a major role in the stimulation of deiodination of hormone, but that once deiodination has occurred, hydrogen peroxide is more important than that superoxide radical in subsequent iodination reactions within the leukocyte.", "PMID": 954676}
{"id": "pubmed23n0032_1254", "title": "Supersensitivity of anterior pituitary dopamine receptors involved in the inhibition of prolactin secretion following destruction of the medial basal hypothalamus.", "content": "The medial basal hypothalamus of ovariectomized rats was destroyed using a modified Hal\u00e1sz knife. Large increases in prolactin secretion were observed 1 and 14 days following the lesions. Long- and short-term lesioned animals were anesthetized with chloral hydrate and treated with various doses of apomorphine (0.05, 0.2, 2, 5 mg/kg). Blood samples were obtained before and 10, 30 and 60 minutes after the injection. Both the 0.05 and 0.2 mg/kg doses caused significantly greater and longer-lasting inhibition of prolactin in long-term than in short-term lesioned animals. Since the MBH was totally destroyed this study suggests that anterior pituitary dopamine receptors involved in the inhibition of prolactin secretion become supersensitive in long-term lesioned rats.", "contents": "Supersensitivity of anterior pituitary dopamine receptors involved in the inhibition of prolactin secretion following destruction of the medial basal hypothalamus. The medial basal hypothalamus of ovariectomized rats was destroyed using a modified Hal\u00e1sz knife. Large increases in prolactin secretion were observed 1 and 14 days following the lesions. Long- and short-term lesioned animals were anesthetized with chloral hydrate and treated with various doses of apomorphine (0.05, 0.2, 2, 5 mg/kg). Blood samples were obtained before and 10, 30 and 60 minutes after the injection. Both the 0.05 and 0.2 mg/kg doses caused significantly greater and longer-lasting inhibition of prolactin in long-term than in short-term lesioned animals. Since the MBH was totally destroyed this study suggests that anterior pituitary dopamine receptors involved in the inhibition of prolactin secretion become supersensitive in long-term lesioned rats.", "PMID": 954677}
{"id": "pubmed23n0032_1255", "title": "Secretion of an FSH-inhibiting factor by cultured Sertoli cells.", "content": "Levels of LH and FSH released in vitro by rat anterior pituitary cells which were either co-cultured with isolated rat Sertoli cells or were grown with spent media recovered from the cultured Sertoli cells were measured by radioimmunoassay. The amounts of FSH released by pituitary cells grown for three days with Sertoli cells isolated from 31-36 day old rats or spent media from the cultured Sertoli cells, were significantly (P less than 0.01) lower compared to control pituitary cultures grown with fresh chemically defined medium. In contrast, the levels of LH were similar to the controls. The selective inhibition of FSH release was not observed when pituitary cells were co-cultured with rat spleen or kidney cells or with ruptured Sertoli cells. The FSH-inhibiting Sertoli-cell factor (SCF) was found to be a heat-labile macromolecule. It is suggested that the SCF may be secreted by the Sertoli cells in vivo, and regulate FSH secretion via negative feedback mechanism at the pituitary level.", "contents": "Secretion of an FSH-inhibiting factor by cultured Sertoli cells. Levels of LH and FSH released in vitro by rat anterior pituitary cells which were either co-cultured with isolated rat Sertoli cells or were grown with spent media recovered from the cultured Sertoli cells were measured by radioimmunoassay. The amounts of FSH released by pituitary cells grown for three days with Sertoli cells isolated from 31-36 day old rats or spent media from the cultured Sertoli cells, were significantly (P less than 0.01) lower compared to control pituitary cultures grown with fresh chemically defined medium. In contrast, the levels of LH were similar to the controls. The selective inhibition of FSH release was not observed when pituitary cells were co-cultured with rat spleen or kidney cells or with ruptured Sertoli cells. The FSH-inhibiting Sertoli-cell factor (SCF) was found to be a heat-labile macromolecule. It is suggested that the SCF may be secreted by the Sertoli cells in vivo, and regulate FSH secretion via negative feedback mechanism at the pituitary level.", "PMID": 954678}
{"id": "pubmed23n0032_1256", "title": "Dual effect of clomiphene citrate on pituitary gonadotropin secretion in postmenopausal women.", "content": "Fifteen postmenopusal women were given 25 mg, 50 mg of clomiphene citrate daily by mouth for 3 consecutive days. Significant (p less than 0.01)decrease of serum LH (73%, 65%) and FSH (76%, 76%) resulted after administration of 25 mg and 50 mg clomiphene citrate respectively. The suppressive effect was sustained for at least 4 days after cessation of this drug. On other hand decreased LH and FSH levels after administration of ethinyl estradiol increased again by simultaneous administration of clomiphene citrate. These results suggest deficient subjects but anti-estrogenic effects are evident when serum estrogen levels are restored in subjects with estrogen deficiency.", "contents": "Dual effect of clomiphene citrate on pituitary gonadotropin secretion in postmenopausal women. Fifteen postmenopusal women were given 25 mg, 50 mg of clomiphene citrate daily by mouth for 3 consecutive days. Significant (p less than 0.01)decrease of serum LH (73%, 65%) and FSH (76%, 76%) resulted after administration of 25 mg and 50 mg clomiphene citrate respectively. The suppressive effect was sustained for at least 4 days after cessation of this drug. On other hand decreased LH and FSH levels after administration of ethinyl estradiol increased again by simultaneous administration of clomiphene citrate. These results suggest deficient subjects but anti-estrogenic effects are evident when serum estrogen levels are restored in subjects with estrogen deficiency.", "PMID": 954679}
{"id": "pubmed23n0032_1257", "title": "Studies on in vitro synthesis and secretion of human chorionic gonadtropin and its subunits.", "content": "Human chorionic tissues were cultivated in vitro in they presence of 3H-proline in order to study the synthesis of the hCG and its subunits by the placenta. After terminating the culture, tissue extracts and media were individually gel-filtrated on Sephadex G-100. The eluted fractions were radioimmunoassayed for hCG, hCGalpha and hCGbeta and were measured for 3H-radioactivity. Label incorporation was determined by immunoprecipitation. Elution profiles of tissue extracts showed the existence of large immunologic forms of hHCG, hCGalpha and hCGbeta emerging near the void volume. The amounts of these large immunologic species in chorionic tissue gradually decreased during the course of cultivation. 3H-proline was almost exclusively incorporated into the large immunologic forms of hCG, hCGalpha and hCGbeta within the chrionic tissue during the 5-hour exposure. A great quantity of hCGalpha was found in the media after the 3-day culture, while the amount of hCGbeta found in the media was minute. After a 15-minute pulse, the 3H-radioactivity peak within the chorionic tissue appeared in the void volume, conicidental with hCG immunoreactivity. During the chase period, there was shift of the 3H-radioactivity peak associated with hCG immunoreactivity from the void volume to the more retarded area. In the media until after the 60-minute chase, no labeled hCG and its subunits appeared. Within the media after 3-hour chase, no labeled hCG peak associated with 3H-radioactivity was more retarded on Sephadex G-100 than that within the tissue extract after the 15-MINUTE pulse. These results suggest that the large immunologic forms of hCG, HCGalpha and hCGbeta are synthesized as the earliest detectable biosynthetic forms and that the may then be converted to small molecule species. In the culture of molar trophoblastic tissues after a 15-minute pulse, considerable amounts of hCG and its subunits accompanied by high 3H-radioactively had already been secreted into the media. These observations suggest that protein synthesis by molar trophoblastic tissue is markedly enhanced as compared with that by normal chorionic tissue and that immunoreactive materials synthesized in molar trophoblastic tissue may be secreted more radily than those synthesized a in normal chorionic tssue.", "contents": "Studies on in vitro synthesis and secretion of human chorionic gonadtropin and its subunits. Human chorionic tissues were cultivated in vitro in they presence of 3H-proline in order to study the synthesis of the hCG and its subunits by the placenta. After terminating the culture, tissue extracts and media were individually gel-filtrated on Sephadex G-100. The eluted fractions were radioimmunoassayed for hCG, hCGalpha and hCGbeta and were measured for 3H-radioactivity. Label incorporation was determined by immunoprecipitation. Elution profiles of tissue extracts showed the existence of large immunologic forms of hHCG, hCGalpha and hCGbeta emerging near the void volume. The amounts of these large immunologic species in chorionic tissue gradually decreased during the course of cultivation. 3H-proline was almost exclusively incorporated into the large immunologic forms of hCG, hCGalpha and hCGbeta within the chrionic tissue during the 5-hour exposure. A great quantity of hCGalpha was found in the media after the 3-day culture, while the amount of hCGbeta found in the media was minute. After a 15-minute pulse, the 3H-radioactivity peak within the chorionic tissue appeared in the void volume, conicidental with hCG immunoreactivity. During the chase period, there was shift of the 3H-radioactivity peak associated with hCG immunoreactivity from the void volume to the more retarded area. In the media until after the 60-minute chase, no labeled hCG and its subunits appeared. Within the media after 3-hour chase, no labeled hCG peak associated with 3H-radioactivity was more retarded on Sephadex G-100 than that within the tissue extract after the 15-MINUTE pulse. These results suggest that the large immunologic forms of hCG, HCGalpha and hCGbeta are synthesized as the earliest detectable biosynthetic forms and that the may then be converted to small molecule species. In the culture of molar trophoblastic tissues after a 15-minute pulse, considerable amounts of hCG and its subunits accompanied by high 3H-radioactively had already been secreted into the media. These observations suggest that protein synthesis by molar trophoblastic tissue is markedly enhanced as compared with that by normal chorionic tissue and that immunoreactive materials synthesized in molar trophoblastic tissue may be secreted more radily than those synthesized a in normal chorionic tssue.", "PMID": 954680}
{"id": "pubmed23n0032_1258", "title": "Intratesticular tissue fluid (ITF) for the micro-bioassay of LH.", "content": "Intratesticular tissue fluid (ITF) was obtained by centrifugation of the decapsulated testis of the adult rat. Testosterone level in ITF was increased by direct injection of LH into the testis and was lowered by anti-LH serum. Based on the result of previous reports with testosterone in testicular vein blood, a simplification of the LH bioassay without imparing sensitivity and accuracy was undertaken using ITF as the material for testosterone measurement. Dose-response relationship between injected LH and testosterone concentration in ITF was observed. The sensitivity of the assay was 5 ng for LH (NIH-LH-S15). FSH (NIH-FSH-S10) and prolactin (NIH-P-S11) had no detectable activity on testosterone production. This assay method depending on biological activities of LH will be helpful for unbiased evaluation of the result obtained by radioimmunoassay.", "contents": "Intratesticular tissue fluid (ITF) for the micro-bioassay of LH. Intratesticular tissue fluid (ITF) was obtained by centrifugation of the decapsulated testis of the adult rat. Testosterone level in ITF was increased by direct injection of LH into the testis and was lowered by anti-LH serum. Based on the result of previous reports with testosterone in testicular vein blood, a simplification of the LH bioassay without imparing sensitivity and accuracy was undertaken using ITF as the material for testosterone measurement. Dose-response relationship between injected LH and testosterone concentration in ITF was observed. The sensitivity of the assay was 5 ng for LH (NIH-LH-S15). FSH (NIH-FSH-S10) and prolactin (NIH-P-S11) had no detectable activity on testosterone production. This assay method depending on biological activities of LH will be helpful for unbiased evaluation of the result obtained by radioimmunoassay.", "PMID": 954681}
{"id": "pubmed23n0032_1259", "title": "Free amino acids in the caput and the cauda epididymis of adult rats.", "content": "To examine the content and the composition of free amino acids in the intraluminal fluid of rat epididymis, the fluids were obtained by light pressure on the dissected tissues. The amount of the total free amino acids in the pressed fluid from the caput epididymis was significantly higher than those of the cauda epididymis and the testis. Glu and Gln were predominant amino acids in the caput, and their amounts occupied more than half of the total ninhydrin reactive compounds. Such a high concentration of Glu and Gln was not observed either in the cauda or in the testis. Castration decreased Glu and increased Gln in amount. Testosterone treatment to castrated animals did not restore Glu and Gln contents in the pressed fluid from the caput epididymis to the level observed in intact rats completely. Therefore, it was assumed that a large amount of Glu in the caput was due to many factors; secretion and metabolism of epithelial cells of the gland which might be regulated by androgen, inflow of rete testis fluid, and sperm metabolism of amino acids in the epididymis. The results obtained from the caput epididymis to which the efferent duct of the testis was ligated also supported this interpretation.", "contents": "Free amino acids in the caput and the cauda epididymis of adult rats. To examine the content and the composition of free amino acids in the intraluminal fluid of rat epididymis, the fluids were obtained by light pressure on the dissected tissues. The amount of the total free amino acids in the pressed fluid from the caput epididymis was significantly higher than those of the cauda epididymis and the testis. Glu and Gln were predominant amino acids in the caput, and their amounts occupied more than half of the total ninhydrin reactive compounds. Such a high concentration of Glu and Gln was not observed either in the cauda or in the testis. Castration decreased Glu and increased Gln in amount. Testosterone treatment to castrated animals did not restore Glu and Gln contents in the pressed fluid from the caput epididymis to the level observed in intact rats completely. Therefore, it was assumed that a large amount of Glu in the caput was due to many factors; secretion and metabolism of epithelial cells of the gland which might be regulated by androgen, inflow of rete testis fluid, and sperm metabolism of amino acids in the epididymis. The results obtained from the caput epididymis to which the efferent duct of the testis was ligated also supported this interpretation.", "PMID": 954682}
{"id": "pubmed23n0032_1260", "title": "Heterotypical sexual behavior in male rats: individual difference in lordosis respones.", "content": "Castrated Wistar male rats were primed with varying amounts of estradiol benzoate (EB) for successive 2 days and progesterone (P) on the third day 6-8 hr prior to the  behavioral test. The tests were performed 4 times at 2-3 weeks intervals. As a priming procedure for the first behavioral test, 50 mug EB and 0.5 mg P were given. The quantity of P was kept constant therafter. For the second test, the dose of EB was increased to 100 mug, but decreased again to 50 mug and further to 10 mug for the third and fourth tests, respectively. The inciedence of animals showing lordosis was quite low, and was not significantly changed by increasing or decreasing the dosage of EB during the series of the behavioral tests. Forty-three out of 51 animals never showed lordosis at all 4 behavioral tests. In contrast, 5 out of 8 rats which responded to mountings by the males continued to display lordosis behavior throughout the series of the successive 4 tests. This consistence of individual responeses during the series of the behavioral tests may indicate the possible existence of individual difference in lordosis responde in male rats.", "contents": "Heterotypical sexual behavior in male rats: individual difference in lordosis respones. Castrated Wistar male rats were primed with varying amounts of estradiol benzoate (EB) for successive 2 days and progesterone (P) on the third day 6-8 hr prior to the  behavioral test. The tests were performed 4 times at 2-3 weeks intervals. As a priming procedure for the first behavioral test, 50 mug EB and 0.5 mg P were given. The quantity of P was kept constant therafter. For the second test, the dose of EB was increased to 100 mug, but decreased again to 50 mug and further to 10 mug for the third and fourth tests, respectively. The inciedence of animals showing lordosis was quite low, and was not significantly changed by increasing or decreasing the dosage of EB during the series of the behavioral tests. Forty-three out of 51 animals never showed lordosis at all 4 behavioral tests. In contrast, 5 out of 8 rats which responded to mountings by the males continued to display lordosis behavior throughout the series of the successive 4 tests. This consistence of individual responeses during the series of the behavioral tests may indicate the possible existence of individual difference in lordosis responde in male rats.", "PMID": 954683}
{"id": "pubmed23n0032_1261", "title": "Immunological studies on LATS-immunogloblin by the reaction with staphylococcal protein A.", "content": "The reaction of LATS activity with Staphylococcal Protein A, a specific binding protein with the Fc part of human IgG(1), IgG(2) and Ig(4), was examined. When IgG(1), IgG(2) and Ig(4) subclasses were removed from LATS positive sera or LATS-IgG fractions by affinity chromatography on Protein A-Sepharose, LATS activity decreased. Almost all LATS activity was found in the fraction that reacted with Protein A. It is suggested that LATS has an expression of a very distinct immunoglobulin G structure, and that LATS activity is distributed mainly in the fraction containing IgG(1), Ig(2) and Ig(4) in LATS positive serum;", "contents": "Immunological studies on LATS-immunogloblin by the reaction with staphylococcal protein A. The reaction of LATS activity with Staphylococcal Protein A, a specific binding protein with the Fc part of human IgG(1), IgG(2) and Ig(4), was examined. When IgG(1), IgG(2) and Ig(4) subclasses were removed from LATS positive sera or LATS-IgG fractions by affinity chromatography on Protein A-Sepharose, LATS activity decreased. Almost all LATS activity was found in the fraction that reacted with Protein A. It is suggested that LATS has an expression of a very distinct immunoglobulin G structure, and that LATS activity is distributed mainly in the fraction containing IgG(1), Ig(2) and Ig(4) in LATS positive serum;", "PMID": 954684}
{"id": "pubmed23n0032_1262", "title": "Effects of synthetic progestational steroids on liver and serum lipids in rats.", "content": "Effects of norethisterone (NT) purified norethisterone (pure NT), norethynodrel (NE), medroxyprogesterone acetate (MAP), chlormadinone acetate (CMA) and desoxycorticosterone acetate (DOCA) on serum and liver lipid levels and serum lipoproteins were examined in both intact and estradiol-treated male rats. NT and NE caused a decrease in serum cholesterol and phospholipid levels, an increase in liver cholesterol level, no significant change in triglyceride levels of both serum and liver, with a significant change in serum lipoprotein patterns; a decreased in alpha- and beta-lipoproteins and a marked increase in pre beta-lipoprotein. Pure NT decreased serum cholesterol without causing any change in lipoprotein pattern. MAP, CMA and DOCA causee almost no effect on lipid levels in serum and liver, but CMA and DOCA increased alpha-lipoprotein and decreased beta- and pre beta-lipoproteins. An acute treatment with estradiol caused a decrease in alpha- and beta-lipoproteins and an increase in pre beta-lipoprotein with a decrease in serum lipid levels and an increase in liver lipids. By contrary, a chronic treatment with a marked hypercholesterolemia. This increase of alpha-lipoprotein in estradiol-treated rats was prevented by NT and NE, not affected or rather decreased by MAP but further increased with CMA and DOCA. These data suggest that the effects of synthetic progestational steroids on lipids are classified into two groups, 19-nortestosterone derivatives and 17alpha-hydroxyprogesterone derivatives including DOCA. The former caused a decrease in serum lipid levels with an increase of pre beta-lipoprotein and adecrease of alpha-lipoprotein. The latter caused almost no change or a slight increase in serum lipid levels with a decrease in pre beta-lipoprotein and an increase in alpha-lipoprotein, though it was not found in MAP.", "contents": "Effects of synthetic progestational steroids on liver and serum lipids in rats. Effects of norethisterone (NT) purified norethisterone (pure NT), norethynodrel (NE), medroxyprogesterone acetate (MAP), chlormadinone acetate (CMA) and desoxycorticosterone acetate (DOCA) on serum and liver lipid levels and serum lipoproteins were examined in both intact and estradiol-treated male rats. NT and NE caused a decrease in serum cholesterol and phospholipid levels, an increase in liver cholesterol level, no significant change in triglyceride levels of both serum and liver, with a significant change in serum lipoprotein patterns; a decreased in alpha- and beta-lipoproteins and a marked increase in pre beta-lipoprotein. Pure NT decreased serum cholesterol without causing any change in lipoprotein pattern. MAP, CMA and DOCA causee almost no effect on lipid levels in serum and liver, but CMA and DOCA increased alpha-lipoprotein and decreased beta- and pre beta-lipoproteins. An acute treatment with estradiol caused a decrease in alpha- and beta-lipoproteins and an increase in pre beta-lipoprotein with a decrease in serum lipid levels and an increase in liver lipids. By contrary, a chronic treatment with a marked hypercholesterolemia. This increase of alpha-lipoprotein in estradiol-treated rats was prevented by NT and NE, not affected or rather decreased by MAP but further increased with CMA and DOCA. These data suggest that the effects of synthetic progestational steroids on lipids are classified into two groups, 19-nortestosterone derivatives and 17alpha-hydroxyprogesterone derivatives including DOCA. The former caused a decrease in serum lipid levels with an increase of pre beta-lipoprotein and adecrease of alpha-lipoprotein. The latter caused almost no change or a slight increase in serum lipid levels with a decrease in pre beta-lipoprotein and an increase in alpha-lipoprotein, though it was not found in MAP.", "PMID": 954685}
{"id": "pubmed23n0032_1263", "title": "Long-term effects of vasectomy on the pituitary-gonadal system of rat and mouse.", "content": "A long-term vasectomy operation in rat and mouse did not reveal any consistent change in the weights of testis and androgen dependent organs. Histological structure of the testis and caput epididymis remains normal after vasectomy. The androgenic activity of the testis remains normal as reflected by fructose content of seminal vesicles. Pituitary cytology remains unaltered. Yellowish cysts were observed frequently in the cauda epididymis of experimental animals, associated with increased epididymal weights. The protein and RNA content of the testis did not show appreciable change. A significant increase was observed in the protein and RNA content of epididymis after bilateral vasectomy. There was no effect of bilateral vasectomy on sialic acid contents of testis and epididymis of rats and seminal vesicular fructose contents of mouse.", "contents": "Long-term effects of vasectomy on the pituitary-gonadal system of rat and mouse. A long-term vasectomy operation in rat and mouse did not reveal any consistent change in the weights of testis and androgen dependent organs. Histological structure of the testis and caput epididymis remains normal after vasectomy. The androgenic activity of the testis remains normal as reflected by fructose content of seminal vesicles. Pituitary cytology remains unaltered. Yellowish cysts were observed frequently in the cauda epididymis of experimental animals, associated with increased epididymal weights. The protein and RNA content of the testis did not show appreciable change. A significant increase was observed in the protein and RNA content of epididymis after bilateral vasectomy. There was no effect of bilateral vasectomy on sialic acid contents of testis and epididymis of rats and seminal vesicular fructose contents of mouse.", "PMID": 954686}
{"id": "pubmed23n0032_1264", "title": "Competitive protein binding assay of oestradiol-17 beta and oestrone using uterine cytosol prepared from HCG-treated rabbits.", "content": "Uterine cytosol was prepared from rabbits after treatment with human chorionic gonadotrophin (HCG) and used for the determination of oestradiol-17 beta and oestrone by competitive protein binding (CPB) assay. The so obtained uterine cytosol gave higher percentage of binding and proved to be more stable when stored at --20 degrees C than cytosol obtained from the pregnant uterus.", "contents": "Competitive protein binding assay of oestradiol-17 beta and oestrone using uterine cytosol prepared from HCG-treated rabbits. Uterine cytosol was prepared from rabbits after treatment with human chorionic gonadotrophin (HCG) and used for the determination of oestradiol-17 beta and oestrone by competitive protein binding (CPB) assay. The so obtained uterine cytosol gave higher percentage of binding and proved to be more stable when stored at --20 degrees C than cytosol obtained from the pregnant uterus.", "PMID": 954687}
{"id": "pubmed23n0032_1265", "title": "Effect of human chorionic gonadotropin on the testicular delta 5-3 beta-hydroxysteriod dehydrogenase and spermatogenesis in hibernating toad (Bufo melanostictus).", "content": "A histochemical study of testicular delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-OHD) and a quantitative study of seminiferous tubules in hibernating toad following the administration of HCG revealed stimulation in the activity of the enzyme delta 5-3 beta-OHD and the spermatogenesis by increasing spermiation, meiotic division and the maturation of secondary spermatogonia.", "contents": "Effect of human chorionic gonadotropin on the testicular delta 5-3 beta-hydroxysteriod dehydrogenase and spermatogenesis in hibernating toad (Bufo melanostictus). A histochemical study of testicular delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-OHD) and a quantitative study of seminiferous tubules in hibernating toad following the administration of HCG revealed stimulation in the activity of the enzyme delta 5-3 beta-OHD and the spermatogenesis by increasing spermiation, meiotic division and the maturation of secondary spermatogonia.", "PMID": 954688}
{"id": "pubmed23n0032_1266", "title": "Comparative investigations on thyroglobulin biosynthesis in rabbit and guinea-pig thyroid in vitro.", "content": "The dynamics of biosynthesis of thyroglobulin (TG) subunits and their polymerization were studied comparatively in rabbit and guinea-pig thyroid in vitro. The incorporation of 14C-leucine into soluble, microsome-bound proteins (total and per mg) and into various fractions of soluble proteins was followed during incubation for 5 to 300 minutes. Some differences in TG synthesis between rabbits and guinea-pigs were observed, namely in the dynamics of 14C-leucine incorporation, the rate of subunit aggregation, the relationship between soluble and particulate proteins and the sedimentation characteristics of newly synthesized and pre-formed TG. The incorporation of 14C-leucine into soluble and microsome-bound proteins increased with time of incubation, but in rabbits the level of incorporation was much higher than in guinea-pigs. In rabbits, the ratio between radioactivity in soluble and particulate proteins decreased with time. In guinea-pig, this ratio increased with time of incubation and was much higher than in rabbits. Sucrose density gradient analysis of the newly formed soluble proteins showed that 12S fraction was synthesized in relatively large amounts, during early incubation of rabbit thyroid, although it was no found as a native protein in the thyroid extract of this species. After prolonged incubation the quantity of 12S protein decreased with a simultaneous increase in the amount of newly formed TG. The quick transformation of 12S subunit into TG is probably the reason why native 12S protein is absent in rabbit thyroid gland. However, in guinea-pigs the transformation into TG is much slower so that a considerable amount of radioactivity remained in the 12S fraction even after 300 min incubation. The results from rabbit experiments suggest that the 12S protein, probably represents a precursor of TG. In guinea-pig, the origin of 12S protein is twofold, as subunit of TG and as a product of its dissociation.", "contents": "Comparative investigations on thyroglobulin biosynthesis in rabbit and guinea-pig thyroid in vitro. The dynamics of biosynthesis of thyroglobulin (TG) subunits and their polymerization were studied comparatively in rabbit and guinea-pig thyroid in vitro. The incorporation of 14C-leucine into soluble, microsome-bound proteins (total and per mg) and into various fractions of soluble proteins was followed during incubation for 5 to 300 minutes. Some differences in TG synthesis between rabbits and guinea-pigs were observed, namely in the dynamics of 14C-leucine incorporation, the rate of subunit aggregation, the relationship between soluble and particulate proteins and the sedimentation characteristics of newly synthesized and pre-formed TG. The incorporation of 14C-leucine into soluble and microsome-bound proteins increased with time of incubation, but in rabbits the level of incorporation was much higher than in guinea-pigs. In rabbits, the ratio between radioactivity in soluble and particulate proteins decreased with time. In guinea-pig, this ratio increased with time of incubation and was much higher than in rabbits. Sucrose density gradient analysis of the newly formed soluble proteins showed that 12S fraction was synthesized in relatively large amounts, during early incubation of rabbit thyroid, although it was no found as a native protein in the thyroid extract of this species. After prolonged incubation the quantity of 12S protein decreased with a simultaneous increase in the amount of newly formed TG. The quick transformation of 12S subunit into TG is probably the reason why native 12S protein is absent in rabbit thyroid gland. However, in guinea-pigs the transformation into TG is much slower so that a considerable amount of radioactivity remained in the 12S fraction even after 300 min incubation. The results from rabbit experiments suggest that the 12S protein, probably represents a precursor of TG. In guinea-pig, the origin of 12S protein is twofold, as subunit of TG and as a product of its dissociation.", "PMID": 954689}
{"id": "pubmed23n0032_1267", "title": "[Diabetes mellitus and Addison's disease)].", "content": "The authors diagnosed diabetes mellitus together with Addison's disease in a 38 year-old male patient. They are discussing therapeutic difficulties arising from the biglandular hypofunctional condition and the problems of the pathogenesis.", "contents": "[Diabetes mellitus and Addison's disease)]. The authors diagnosed diabetes mellitus together with Addison's disease in a 38 year-old male patient. They are discussing therapeutic difficulties arising from the biglandular hypofunctional condition and the problems of the pathogenesis.", "PMID": 954690}
{"id": "pubmed23n0032_1268", "title": "Lengthening of the duration of action of DDAVP by \"ineffective\" doses of clofibrate in patients with pituitary diabetes insipidus.", "content": "Duration of action of single intravenous and intranasal doses of 1-Deamino-8D-Arginine Vasopressin (DDAVP) was lengthened by \"ineffective\" doses of clofibrate (not affecting water metabolism when administered alone) in patients with pituitary diabetes insipidus. This interaction was observed when as low doses as 0.02 mug DDAVP were administered intravenously to one patient and as high doses as 1 and 2 mug DDAVP were given intravenously to another patient. Administration of less or more DDAVP than \"optimal\" for the interaction resulted in decreased or absent potentiation by clofibrate. The exact cause of the interaction is unknown.", "contents": "Lengthening of the duration of action of DDAVP by \"ineffective\" doses of clofibrate in patients with pituitary diabetes insipidus. Duration of action of single intravenous and intranasal doses of 1-Deamino-8D-Arginine Vasopressin (DDAVP) was lengthened by \"ineffective\" doses of clofibrate (not affecting water metabolism when administered alone) in patients with pituitary diabetes insipidus. This interaction was observed when as low doses as 0.02 mug DDAVP were administered intravenously to one patient and as high doses as 1 and 2 mug DDAVP were given intravenously to another patient. Administration of less or more DDAVP than \"optimal\" for the interaction resulted in decreased or absent potentiation by clofibrate. The exact cause of the interaction is unknown.", "PMID": 954691}
{"id": "pubmed23n0032_1269", "title": "Treatment of diabetic coma with low-dose injections of insulin.", "content": "Twenty-one patients in severe diabetic coma were treated with small doses of insulin at a rate of 4.1 units per hour (total dose about 100 units per 24 hours). Using single doses of 4 to 10 units by the intravenous or intramuscular routes the fall of blood glucose was steady in all cases. In the treatment of diabetic coma this regimen of insulin administration has proved simple, safe and effective since 1946. Main dangers during recompensation of diabetic coma are: hypovolaemia with oliguria -- anuria, dysequilibrium syndrome with cerebral edema and hypokalaemia. Therefore early intensive and adequate intravenous fluid and electrolyte replacement is the most important part of treatment. Most of the cases in this study were undiagnosed diabetics (14) and elderly patients (9). Three patients older than 65 years and a 56-year old diabetic died. In this context the most important aspects of treatment to avoid death are: prevention of diabetic coma and adequate fluid and electrolyte replacement especially in geriatric patients.", "contents": "Treatment of diabetic coma with low-dose injections of insulin. Twenty-one patients in severe diabetic coma were treated with small doses of insulin at a rate of 4.1 units per hour (total dose about 100 units per 24 hours). Using single doses of 4 to 10 units by the intravenous or intramuscular routes the fall of blood glucose was steady in all cases. In the treatment of diabetic coma this regimen of insulin administration has proved simple, safe and effective since 1946. Main dangers during recompensation of diabetic coma are: hypovolaemia with oliguria -- anuria, dysequilibrium syndrome with cerebral edema and hypokalaemia. Therefore early intensive and adequate intravenous fluid and electrolyte replacement is the most important part of treatment. Most of the cases in this study were undiagnosed diabetics (14) and elderly patients (9). Three patients older than 65 years and a 56-year old diabetic died. In this context the most important aspects of treatment to avoid death are: prevention of diabetic coma and adequate fluid and electrolyte replacement especially in geriatric patients.", "PMID": 954692}
{"id": "pubmed23n0032_1270", "title": "[Stimulation of ovarian function in infantile rats with testosterone alone or in combination with chorionic gonadotropin or clomiphene citrate].", "content": "Infantile female rats were subcutaneously injected with 2.5 mg testosterone within two days. The majority of the rats responded to this treatment with opening of the vagina, and about 50% developed an estrus. However, corpora lutea were not formed. Equally treated animals received additionally 0.2 i.u. HCG -- a dose which is inffective when given alone -- distributed on the second and third day of experiment. After this treatment an increased number of rats reacted with estrus and the development of corpora lutea. In infantile female rats, follicles stimulated by testosterone were also transformed into corpora lutea by an injection of 0.025 mg clomiphene citrate.", "contents": "[Stimulation of ovarian function in infantile rats with testosterone alone or in combination with chorionic gonadotropin or clomiphene citrate]. Infantile female rats were subcutaneously injected with 2.5 mg testosterone within two days. The majority of the rats responded to this treatment with opening of the vagina, and about 50% developed an estrus. However, corpora lutea were not formed. Equally treated animals received additionally 0.2 i.u. HCG -- a dose which is inffective when given alone -- distributed on the second and third day of experiment. After this treatment an increased number of rats reacted with estrus and the development of corpora lutea. In infantile female rats, follicles stimulated by testosterone were also transformed into corpora lutea by an injection of 0.025 mg clomiphene citrate.", "PMID": 954693}
{"id": "pubmed23n0032_1271", "title": "Histochemical observations on the adrenal gland of bat Vesperugo pipistrellus (Dobson).", "content": "The presence of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-HSDH), 11beta-hydroxysteroid dehydrogenase 11beta-HSDH), 17beta-hydroxysteroid dehydrogenase (17beta-HSDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) has been histochemically demonstrated in the zona glomerulosa, fasciculata and reticularis and also in the cortical cells that are found interspersed in the medulla of the adrenal gland of the bat, Vesperugo pipistrellus. The present study indicates that the adrenal cortex of the bat is capable of synthesizing corticosteroids and also sex steroids.", "contents": "Histochemical observations on the adrenal gland of bat Vesperugo pipistrellus (Dobson). The presence of delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta-HSDH), 11beta-hydroxysteroid dehydrogenase 11beta-HSDH), 17beta-hydroxysteroid dehydrogenase (17beta-HSDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) has been histochemically demonstrated in the zona glomerulosa, fasciculata and reticularis and also in the cortical cells that are found interspersed in the medulla of the adrenal gland of the bat, Vesperugo pipistrellus. The present study indicates that the adrenal cortex of the bat is capable of synthesizing corticosteroids and also sex steroids.", "PMID": 954694}
{"id": "pubmed23n0032_1272", "title": "Regulation of nitrogen catabolic enzymes in chick liver -- effects of amino acids.", "content": "It is well-known that in the chick, dietary protein increases the levels of several hepatic enzymes that are involved in nitrogen metabolism and excretion. However, the biochemical mechanism of this response is essentially unknown. The experiments presented in this paper show that the chick is responding to alpha-amino nitrogen and not to any specific amino acid. Furthermore, it is shown that this system responds to endogenous sources of nitrogen as well as dietary protein and that the xanthine dehydrogenase response involves regulation of enzyme synthesis without changing the rate of degradation.", "contents": "Regulation of nitrogen catabolic enzymes in chick liver -- effects of amino acids. It is well-known that in the chick, dietary protein increases the levels of several hepatic enzymes that are involved in nitrogen metabolism and excretion. However, the biochemical mechanism of this response is essentially unknown. The experiments presented in this paper show that the chick is responding to alpha-amino nitrogen and not to any specific amino acid. Furthermore, it is shown that this system responds to endogenous sources of nitrogen as well as dietary protein and that the xanthine dehydrogenase response involves regulation of enzyme synthesis without changing the rate of degradation.", "PMID": 954712}
{"id": "pubmed23n0032_1273", "title": "Changes in hepatic fatty acid degradation and blood lipid and ketone body content during development of the rat.", "content": "The following have been measured during the development of the laboratory rat: the rate of oxidation of palmitoylcarnitine, decanoylcarnitine, and 14C-palmitate by liver mitochondria; the concentrations of ketone bodies in the blood; the plasma concentrations of non-esterified fatty acids, glycerol, and triglyceride. In each case, a rise after birth and a fall at weaning were observed. These changes can be correlated with the dietary changes which occur at these times. However, during the suckling period, when a constant high fat content diet is consumed, further marked changes in the parameters measured were observed which cannot be related to nutritional factors.", "contents": "Changes in hepatic fatty acid degradation and blood lipid and ketone body content during development of the rat. The following have been measured during the development of the laboratory rat: the rate of oxidation of palmitoylcarnitine, decanoylcarnitine, and 14C-palmitate by liver mitochondria; the concentrations of ketone bodies in the blood; the plasma concentrations of non-esterified fatty acids, glycerol, and triglyceride. In each case, a rise after birth and a fall at weaning were observed. These changes can be correlated with the dietary changes which occur at these times. However, during the suckling period, when a constant high fat content diet is consumed, further marked changes in the parameters measured were observed which cannot be related to nutritional factors.", "PMID": 954713}
{"id": "pubmed23n0032_1274", "title": "A comparative study of glytaminase isozymes in rat tissues.", "content": "The three kinds of glutamineses in rat kidney and liver were defined in comparative terms by their properties and were separately purified. Substantial purification was obtained by polymerization and depolymerization of the kidney isozyme that is activated by phosphate, These isozymes differ most strikingly in the activators of their reactions:maleate and a high concentration of phosphate, respectively, for the two kidney isozymes, and a low concentration of phosphate for the liver isozyme. The kidney isozyme that is activated by phosphate was also activated by a much lower concentration of ATP and by other complex phosphates. They also differ in physical properties: the maleate-activated isozyme was heat resistant (50 degrees C) and associated with insoluble submitochondrial particles; both phosphate-activated isozymes were heat sensitive and could be solubilized from their respective mitochondria; the phosphate-activated isozyme of kidney polymerized in phosphate-borate solution while that of liver did not. The characteristics of the kidney isozyme that was activated by high phosphate were shared by the glutaminases in adult brain, transplanted tumors, and in fetal liver and kidney. The similarity to the kidney enzyme was confirmed by the use of polymerization in phosphate borate of the isozyme from a mammary carcinoma to effect its purification.", "contents": "A comparative study of glytaminase isozymes in rat tissues. The three kinds of glutamineses in rat kidney and liver were defined in comparative terms by their properties and were separately purified. Substantial purification was obtained by polymerization and depolymerization of the kidney isozyme that is activated by phosphate, These isozymes differ most strikingly in the activators of their reactions:maleate and a high concentration of phosphate, respectively, for the two kidney isozymes, and a low concentration of phosphate for the liver isozyme. The kidney isozyme that is activated by phosphate was also activated by a much lower concentration of ATP and by other complex phosphates. They also differ in physical properties: the maleate-activated isozyme was heat resistant (50 degrees C) and associated with insoluble submitochondrial particles; both phosphate-activated isozymes were heat sensitive and could be solubilized from their respective mitochondria; the phosphate-activated isozyme of kidney polymerized in phosphate-borate solution while that of liver did not. The characteristics of the kidney isozyme that was activated by high phosphate were shared by the glutaminases in adult brain, transplanted tumors, and in fetal liver and kidney. The similarity to the kidney enzyme was confirmed by the use of polymerization in phosphate borate of the isozyme from a mammary carcinoma to effect its purification.", "PMID": 954714}
{"id": "pubmed23n0032_1275", "title": "The development of creatine kinase in rat skeletal muscle. Changes in isoenzyme ratio, protein, RNA and DNA during development.", "content": "The activity of cytosolic creatine kinase in rat skeletal muscle rises stepwise during development. The increases occur simultnaeously with transient increases in DNA content. The second increase is accompanied by a rise in total protein, soluble sarcoplasmic protein and RNA/DNA ratio. Such changes are not observed at 20 days after birth, when creatine kinase finally accumulates to the adult level. Transient higher amounts of the MB and BB isoenzymes are observed after the first and second stepwise increase. The increase in creatine kinase activity observed after birth is predominantly due to an activation of the M gene. The BB isoenzyme is still present in adult skeletal muscle, but contributes little to the total activity.", "contents": "The development of creatine kinase in rat skeletal muscle. Changes in isoenzyme ratio, protein, RNA and DNA during development. The activity of cytosolic creatine kinase in rat skeletal muscle rises stepwise during development. The increases occur simultnaeously with transient increases in DNA content. The second increase is accompanied by a rise in total protein, soluble sarcoplasmic protein and RNA/DNA ratio. Such changes are not observed at 20 days after birth, when creatine kinase finally accumulates to the adult level. Transient higher amounts of the MB and BB isoenzymes are observed after the first and second stepwise increase. The increase in creatine kinase activity observed after birth is predominantly due to an activation of the M gene. The BB isoenzyme is still present in adult skeletal muscle, but contributes little to the total activity.", "PMID": 954715}
{"id": "pubmed23n0032_1276", "title": "Nature of the multiple forms of alanine aminopeptidase.", "content": "Alanine aminopeptidase (particle-bound aminopeptidase, EC 3.4.11.2) isolated in a highly purified state from human liver, kidney, and pancreas were investigated with regard to their different electrophoretic mobilities. While the molecular weights are identical, different isoelectric points were found. The results of carbohydrate analysis point to N-acetylneuraminic acid as the main factor that causes the differences in electrophoretic behaviour of the alanine aminopeptidases investigated.", "contents": "Nature of the multiple forms of alanine aminopeptidase. Alanine aminopeptidase (particle-bound aminopeptidase, EC 3.4.11.2) isolated in a highly purified state from human liver, kidney, and pancreas were investigated with regard to their different electrophoretic mobilities. While the molecular weights are identical, different isoelectric points were found. The results of carbohydrate analysis point to N-acetylneuraminic acid as the main factor that causes the differences in electrophoretic behaviour of the alanine aminopeptidases investigated.", "PMID": 954716}
{"id": "pubmed23n0032_1277", "title": "Percutaneous tendon splitting--method and results.", "content": "The paper briefly describes the percutaneous tendon splitting operation as performed in general practice and evaluates its effectiveness on the basis of races run during a 2 year follow-up period. The results are compared with similar figures for normal Standardbred trotters. A higher percentage of horses subjected to splitting of the suspensory ligament failed to remain sound long enough to run more than 10 races during the follow-up period. The percentage of horses returning to racing whose superficial flexor was operated upon was somewhat lower than the control group.", "contents": "Percutaneous tendon splitting--method and results. The paper briefly describes the percutaneous tendon splitting operation as performed in general practice and evaluates its effectiveness on the basis of races run during a 2 year follow-up period. The results are compared with similar figures for normal Standardbred trotters. A higher percentage of horses subjected to splitting of the suspensory ligament failed to remain sound long enough to run more than 10 races during the follow-up period. The percentage of horses returning to racing whose superficial flexor was operated upon was somewhat lower than the control group.", "PMID": 954717}
{"id": "pubmed23n0032_1278", "title": "Fractures of the pedal bone in the horse.", "content": "A series of 79 fractures of the pedal bone in various types of horses are reported and the clinical features described. The fractures were of 3 types--intra-articular or extra-articular, involving the wing of the pedal bone, and fractures of the extensor process. Forty-five horses with a fractured wing of the pedal bone were treated conservatively and 11 by immobilisation of the bony fragment with a compression screw. Conservative treatment was usually successful in the extra-articular fractures. Intra-articular fractures treated conservatively in animals under 3 years had a good prognosis. The results were not so satisfactory in older horses and surgical treatment proved to be the method of choice. All, but one, of the 23 fractures of the extensor process were treated conservatively. The prognosis was not always good, as secondary osteoarthritis of the interphalangeal joint sometimes developed.", "contents": "Fractures of the pedal bone in the horse. A series of 79 fractures of the pedal bone in various types of horses are reported and the clinical features described. The fractures were of 3 types--intra-articular or extra-articular, involving the wing of the pedal bone, and fractures of the extensor process. Forty-five horses with a fractured wing of the pedal bone were treated conservatively and 11 by immobilisation of the bony fragment with a compression screw. Conservative treatment was usually successful in the extra-articular fractures. Intra-articular fractures treated conservatively in animals under 3 years had a good prognosis. The results were not so satisfactory in older horses and surgical treatment proved to be the method of choice. All, but one, of the 23 fractures of the extensor process were treated conservatively. The prognosis was not always good, as secondary osteoarthritis of the interphalangeal joint sometimes developed.", "PMID": 954718}
{"id": "pubmed23n0032_1279", "title": "Epidermoid cyst of the brain in the horse.", "content": "An aged Connemara mare had a neurological illness lasting 3 weeks and characterised by episodes of dullness, head-pressing, staggering gait and hyperexcitability. The signs recurred at intervals of 3 to 4 days, with intervening periods of apparent normality. Post-mortem examination revealed an epidermoid cyst on the dorsal surface of the occipital lobe of the left cerebral hemisphere. The cyst was lined by stratified squamous epithelium and contained desquamated keratinised material and blood. The lesion is considered to be a congenital malformation which enlarges and produces space-occupying signs.", "contents": "Epidermoid cyst of the brain in the horse. An aged Connemara mare had a neurological illness lasting 3 weeks and characterised by episodes of dullness, head-pressing, staggering gait and hyperexcitability. The signs recurred at intervals of 3 to 4 days, with intervening periods of apparent normality. Post-mortem examination revealed an epidermoid cyst on the dorsal surface of the occipital lobe of the left cerebral hemisphere. The cyst was lined by stratified squamous epithelium and contained desquamated keratinised material and blood. The lesion is considered to be a congenital malformation which enlarges and produces space-occupying signs.", "PMID": 954719}
{"id": "pubmed23n0032_1280", "title": "Four cases of Tyzzer's disease in foals in England.", "content": "A rodent pathogen, Bacillus piliformis, has been recognised as causing a rapidly fatal hepatitis in 4 foals in England. The disease in foals has been recognised in America since 1973. A clinico-pathological account of the 4 cases is given and the differential diagnosis discussed. The 4 foals' ages fell within a very narrow range (24-34 days). Some of the properties of this unusual intracellular pathogen are reviewed. For the first time in the equine the bacillus was seen in association with myocardial lesions. There are marked differences in the epidemiology of the disease in the mouse and in the foal, which may result from the very different management practices in the 2 species. It is suggested that the dams of affected foals may be carriers of the disease.", "contents": "Four cases of Tyzzer's disease in foals in England. A rodent pathogen, Bacillus piliformis, has been recognised as causing a rapidly fatal hepatitis in 4 foals in England. The disease in foals has been recognised in America since 1973. A clinico-pathological account of the 4 cases is given and the differential diagnosis discussed. The 4 foals' ages fell within a very narrow range (24-34 days). Some of the properties of this unusual intracellular pathogen are reviewed. For the first time in the equine the bacillus was seen in association with myocardial lesions. There are marked differences in the epidemiology of the disease in the mouse and in the foal, which may result from the very different management practices in the 2 species. It is suggested that the dams of affected foals may be carriers of the disease.", "PMID": 954720}
{"id": "pubmed23n0032_1281", "title": "A practical health programme for prevention of parasitic and infectious diseases in horses and ponies.", "content": "A scheme of combined anthelmintic treatment and vaccination is described. The seasonal veterinary activities are as follows: March (all horses); parasitological examination of faeces, anthelmintic treatment, immunization against influenza and tetanus (booster dose). June: parasitological faeces examination of foals, anthelmintic treatment of all horses, immunization of foals against tetanus (1st dose). August: anthelmintic treatment of all horses and immunization of foals against influenza (1st dose) and tetanus (2nd dose). October: parasitological faeces examination and anthelmintic treatment of all horses, and immunization against influenza of foals (2nd dose) and yearlings (2nd booster dose). December (for all horses): anthelmintic treatment.", "contents": "A practical health programme for prevention of parasitic and infectious diseases in horses and ponies. A scheme of combined anthelmintic treatment and vaccination is described. The seasonal veterinary activities are as follows: March (all horses); parasitological examination of faeces, anthelmintic treatment, immunization against influenza and tetanus (booster dose). June: parasitological faeces examination of foals, anthelmintic treatment of all horses, immunization of foals against tetanus (1st dose). August: anthelmintic treatment of all horses and immunization of foals against influenza (1st dose) and tetanus (2nd dose). October: parasitological faeces examination and anthelmintic treatment of all horses, and immunization against influenza of foals (2nd dose) and yearlings (2nd booster dose). December (for all horses): anthelmintic treatment.", "PMID": 954721}
{"id": "pubmed23n0032_1282", "title": "The importance of blood gas measurement in the diagnosis of an intraventricular septal defect in a horse: a case report.", "content": "An 18 month Thoroughbred gelding was diagnosed on cardiac catheterization as having an interventricular septal defect. Right side cardiac blood pressures were within the normal range and confirmation of the diagnosis came from changes in the blood pO2 and pCO2 between the right atrium and right ventricle. The significance of these values is discussed.", "contents": "The importance of blood gas measurement in the diagnosis of an intraventricular septal defect in a horse: a case report. An 18 month Thoroughbred gelding was diagnosed on cardiac catheterization as having an interventricular septal defect. Right side cardiac blood pressures were within the normal range and confirmation of the diagnosis came from changes in the blood pO2 and pCO2 between the right atrium and right ventricle. The significance of these values is discussed.", "PMID": 954722}
{"id": "pubmed23n0032_1283", "title": "Growth rate in thoroughbred yearlings and two year olds.", "content": "One hundred and four animals aged 13-18 months were measured at monthly or two monthly intervals, as were comparable numbers of animals 19-36 months old. Height, girth and circumference of the cannon bone below the knee were recorded, measurements for colts and fillies are shown separately. The average height at 18 months was 148 cm (15.01/2 h.h.) and at 36 months 156 cm (15.31/2 h.h.).", "contents": "Growth rate in thoroughbred yearlings and two year olds. One hundred and four animals aged 13-18 months were measured at monthly or two monthly intervals, as were comparable numbers of animals 19-36 months old. Height, girth and circumference of the cannon bone below the knee were recorded, measurements for colts and fillies are shown separately. The average height at 18 months was 148 cm (15.01/2 h.h.) and at 36 months 156 cm (15.31/2 h.h.).", "PMID": 954724}
{"id": "pubmed23n0032_1284", "title": "The adipokinetic effect of hyperthermic stress in man.", "content": "The purpose of this investigation was to assess the alteration in serum free fatty acid concentrations during heat stress and dehydration. Each subject was exposed to heat stress in an environment chamber on 2 separate occasions. During the first exposure the subjects remained seated until the core temperature was elevated 1.4degreesC resulting in a mean weight loss of 1.66 kg due to dehydration. The second condition involved water replacement equal to the weight loss of the initial dehydration condition. Blood samples were obtained prior to heat exposure, when the core temperature was elevated 0.7degreesC and 1.4degreesC. They were subsequently analyzed for free fatty acids (FFA), glucose and lactin acid. Heart rates and core temperatures were monitored at 4 min intervals. During the dehydration condition the mean change in serum FFA was 0.9 muEq/ml in contrast to 0.2 muEq/ml for the rehydration condition. Serum levels of glucose increased moderately throughout the exposure (8 mg-%).", "contents": "The adipokinetic effect of hyperthermic stress in man. The purpose of this investigation was to assess the alteration in serum free fatty acid concentrations during heat stress and dehydration. Each subject was exposed to heat stress in an environment chamber on 2 separate occasions. During the first exposure the subjects remained seated until the core temperature was elevated 1.4degreesC resulting in a mean weight loss of 1.66 kg due to dehydration. The second condition involved water replacement equal to the weight loss of the initial dehydration condition. Blood samples were obtained prior to heat exposure, when the core temperature was elevated 0.7degreesC and 1.4degreesC. They were subsequently analyzed for free fatty acids (FFA), glucose and lactin acid. Heart rates and core temperatures were monitored at 4 min intervals. During the dehydration condition the mean change in serum FFA was 0.9 muEq/ml in contrast to 0.2 muEq/ml for the rehydration condition. Serum levels of glucose increased moderately throughout the exposure (8 mg-%).", "PMID": 954727}
{"id": "pubmed23n0032_1285", "title": "Structure and function of cardiac mitochondria in exhausted guinea pigs.", "content": "The ultrastructure and functional capacity of mitochondria from hearts of exhausted guinea pigs were compared to those from rested animals. Electron micrographs of heart ventricles were examined for ultrastructural alterations, and functional capacity was studied by oxygen polarography. General swelling of mitochondria from exhausted animals was indicated by a 41% reduction in mitochondrial surface/volume ratio and a 22% increase in volume density of mitochondria from exercised animals when compared to those from controls. The yield of isolated mitochondria from exhausted guinea pigs was similar to that for control animals. In the presence of all substrates tested the rate of oxygen consumption was lower in the exhausted animals than in the controls. The ADP/O ratios and respiratory control indices for pyruvate, succinate and ascorbate were not systematically affected by exercise. With glutamate as substrate respiratory control was reduced in exercised guinea pigs. These experiments suggest that the structure of myocardial mitochondria from exhausted animals is sufficient to maintain normal ADP/O ratios but is not adequate to maintain normal rates of respiration.", "contents": "Structure and function of cardiac mitochondria in exhausted guinea pigs. The ultrastructure and functional capacity of mitochondria from hearts of exhausted guinea pigs were compared to those from rested animals. Electron micrographs of heart ventricles were examined for ultrastructural alterations, and functional capacity was studied by oxygen polarography. General swelling of mitochondria from exhausted animals was indicated by a 41% reduction in mitochondrial surface/volume ratio and a 22% increase in volume density of mitochondria from exercised animals when compared to those from controls. The yield of isolated mitochondria from exhausted guinea pigs was similar to that for control animals. In the presence of all substrates tested the rate of oxygen consumption was lower in the exhausted animals than in the controls. The ADP/O ratios and respiratory control indices for pyruvate, succinate and ascorbate were not systematically affected by exercise. With glutamate as substrate respiratory control was reduced in exercised guinea pigs. These experiments suggest that the structure of myocardial mitochondria from exhausted animals is sufficient to maintain normal ADP/O ratios but is not adequate to maintain normal rates of respiration.", "PMID": 954728}
{"id": "pubmed23n0032_1286", "title": "Effects of physical training on ventilatory equivalent and respiratory exchange ratio during weight supported, steady-state exercise.", "content": "Thirty-three college men participated in a 9-week endurance training program. An equal number of subjects served as controls. Pre- and post-test metabolic measurements were made during 10 min of submaximal exercise (1080 kpm/min at 60 rpm) and 15 min of recovery. Measurements included oxygen consumption, CO2 production, ventilatory equivalent (Ve/VO2 ratio) and respiratory exchange ratio (R). A three factor design variance analysis was used to analyze the effects of training on min-by-min exercise and recovery Ve/VO2 ratio and R. For the experimental group training resulted in a significant improvement in ventilatory efficiency during exercise, as well as a significant decrease in R. During recovery, Ve/VO2 and R decreased significantly for both groups although the magnitude of change was greater for the group that trained. Apparently, there was a significant habituation effect due to test procedures for the control group. The results are discussed in terms of lactate production and substrate utilization during exercise.", "contents": "Effects of physical training on ventilatory equivalent and respiratory exchange ratio during weight supported, steady-state exercise. Thirty-three college men participated in a 9-week endurance training program. An equal number of subjects served as controls. Pre- and post-test metabolic measurements were made during 10 min of submaximal exercise (1080 kpm/min at 60 rpm) and 15 min of recovery. Measurements included oxygen consumption, CO2 production, ventilatory equivalent (Ve/VO2 ratio) and respiratory exchange ratio (R). A three factor design variance analysis was used to analyze the effects of training on min-by-min exercise and recovery Ve/VO2 ratio and R. For the experimental group training resulted in a significant improvement in ventilatory efficiency during exercise, as well as a significant decrease in R. During recovery, Ve/VO2 and R decreased significantly for both groups although the magnitude of change was greater for the group that trained. Apparently, there was a significant habituation effect due to test procedures for the control group. The results are discussed in terms of lactate production and substrate utilization during exercise.", "PMID": 954729}
{"id": "pubmed23n0032_1287", "title": "Oxygen deficit and repayment in submaximal exercise.", "content": "Oxygen deficit and repayment ratios were investigated at various work loads, intensities and durations. An active baseline was used (walking at 60 m/min) from which deficit and repayment values were calculated. Oxygen uptake (VO2) and core temperatures were measured in 30 males at baseline and during treadmill running (140 m/min) for randomly assigned durations (0.5 ...20 min). Measurements were also made during a 30-min recovery period at baseline work. Results indicated: 1) No difference in O2-repayment between steady-state work and work prior to steady state (P greater than 0.10). 2) O2-repayment was independent of work duration (P greater than 0.10). 3) When workload and intensity were controlled, O2-deficit was not significant factor in O2-repayment (P greater than 0.10). 4) Work intensity (work VO2/VO2 max) was the most significant factor in O2-repayment accounting for 69% of the variance (r equals 0.83, P less than 0.001). Small increments in core temperature and ventilation were not significant factors in O2-repayment. When a working baseline is used, the magnitude of O2-repayment after exercise is independent of the work duration or the attainment of steady state. The extent of O2-repayment after exercise is mainly dependent upon the physiological intensity of the work and the absolute workload (R=0.89, P less than 0.001).", "contents": "Oxygen deficit and repayment in submaximal exercise. Oxygen deficit and repayment ratios were investigated at various work loads, intensities and durations. An active baseline was used (walking at 60 m/min) from which deficit and repayment values were calculated. Oxygen uptake (VO2) and core temperatures were measured in 30 males at baseline and during treadmill running (140 m/min) for randomly assigned durations (0.5 ...20 min). Measurements were also made during a 30-min recovery period at baseline work. Results indicated: 1) No difference in O2-repayment between steady-state work and work prior to steady state (P greater than 0.10). 2) O2-repayment was independent of work duration (P greater than 0.10). 3) When workload and intensity were controlled, O2-deficit was not significant factor in O2-repayment (P greater than 0.10). 4) Work intensity (work VO2/VO2 max) was the most significant factor in O2-repayment accounting for 69% of the variance (r equals 0.83, P less than 0.001). Small increments in core temperature and ventilation were not significant factors in O2-repayment. When a working baseline is used, the magnitude of O2-repayment after exercise is independent of the work duration or the attainment of steady state. The extent of O2-repayment after exercise is mainly dependent upon the physiological intensity of the work and the absolute workload (R=0.89, P less than 0.001).", "PMID": 954730}
{"id": "pubmed23n0032_1288", "title": "[Examination of blinking-rate in a simulated four hour driving task].", "content": "In a driving simulation task performance and physiological measurement of strain was registered on 3 subjects in 9 experiments. The time-variant behaviour of blinking-rate was examined together with performance, self-rating and physiological strain. The correlating results make blinking-rate a possible indicator for activation of willingness to good performance. A multidimensional concept for assessment of the degree of individual fatigue is presented and validated in respect of working-time.", "contents": "[Examination of blinking-rate in a simulated four hour driving task]. In a driving simulation task performance and physiological measurement of strain was registered on 3 subjects in 9 experiments. The time-variant behaviour of blinking-rate was examined together with performance, self-rating and physiological strain. The correlating results make blinking-rate a possible indicator for activation of willingness to good performance. A multidimensional concept for assessment of the degree of individual fatigue is presented and validated in respect of working-time.", "PMID": 954731}
{"id": "pubmed23n0032_1289", "title": "Motor unit differentiation and integrated surface EMG in voluntary isometric contraction.", "content": "The role of the differentiation of motor units (MU's) in the relation between integrated surface electromydgram and force was studied here for the biceps brachii, in the course of static contraction in man. The global EMG of the biceps brachii was recorded by two silver electrodes fixed to the skin. The MU's activity was simultaneously lead off by three bipolar wire electrodes inserted in three points of the muscle. MU firing frequency and recruitment were estimated by counting the action potentials on the three elementary records. The comparison between the increase of the integrated surface EMG and MU recruitment as a function of force shows that MU recruitment in itself cannot account for the increase in the integrated global EMG, particularly for high values of force. The difference between MU recruitmentand integrated global EMG, which can neither come from a possible MU's synchronisation as was shown in the discussion, is being suggested to be connected to the progressive firing of \"phasic\" MU's. This type of recruitment may also play an important role in the gradation of isometric force in normal man.", "contents": "Motor unit differentiation and integrated surface EMG in voluntary isometric contraction. The role of the differentiation of motor units (MU's) in the relation between integrated surface electromydgram and force was studied here for the biceps brachii, in the course of static contraction in man. The global EMG of the biceps brachii was recorded by two silver electrodes fixed to the skin. The MU's activity was simultaneously lead off by three bipolar wire electrodes inserted in three points of the muscle. MU firing frequency and recruitment were estimated by counting the action potentials on the three elementary records. The comparison between the increase of the integrated surface EMG and MU recruitment as a function of force shows that MU recruitment in itself cannot account for the increase in the integrated global EMG, particularly for high values of force. The difference between MU recruitmentand integrated global EMG, which can neither come from a possible MU's synchronisation as was shown in the discussion, is being suggested to be connected to the progressive firing of \"phasic\" MU's. This type of recruitment may also play an important role in the gradation of isometric force in normal man.", "PMID": 954732}
{"id": "pubmed23n0032_1290", "title": "Hemodynamic responses to acute hematocrit and blood volume alterations in rats.", "content": "Previous investigations of polycythemia in rodents failed to delineate the effects of the increased hematocrits from the effects of possible changes in blood volume. The hemodynamic responses of anesthetized rats to acutely produced increases in hematocrit and blood volume were therefore studied in this experiment. Acute isovolumetric polycythemia decreased O2 transport from a control value of 44.9 to 35.1 ml/min/kg 60 min after the exchange-transfusion. However, the high venous O2 content (over 12 Vol.-%) following the production of isovolumetric polycythemia suggested that there was still sufficient oxygen available to the tissues. Hypervolemia significantly modified the response to acute polycythemia. When hematocrit was maintained at a constant level, acute hypervolemia increased O2 transport (to 54.6 ml/min/kg after 60 min), and when acute hypervolemia and polycythemia were induced simultaneously, O2 transport was transiently increased (to 57.5 ml/min/kg after 15 min) and then returned to a value similar to the control level.", "contents": "Hemodynamic responses to acute hematocrit and blood volume alterations in rats. Previous investigations of polycythemia in rodents failed to delineate the effects of the increased hematocrits from the effects of possible changes in blood volume. The hemodynamic responses of anesthetized rats to acutely produced increases in hematocrit and blood volume were therefore studied in this experiment. Acute isovolumetric polycythemia decreased O2 transport from a control value of 44.9 to 35.1 ml/min/kg 60 min after the exchange-transfusion. However, the high venous O2 content (over 12 Vol.-%) following the production of isovolumetric polycythemia suggested that there was still sufficient oxygen available to the tissues. Hypervolemia significantly modified the response to acute polycythemia. When hematocrit was maintained at a constant level, acute hypervolemia increased O2 transport (to 54.6 ml/min/kg after 60 min), and when acute hypervolemia and polycythemia were induced simultaneously, O2 transport was transiently increased (to 57.5 ml/min/kg after 15 min) and then returned to a value similar to the control level.", "PMID": 954733}
{"id": "pubmed23n0032_1291", "title": "Step increment versus constant load tests for determination of maximal oxygen uptake.", "content": "Ten male subjects performed five maximal treadmill running tests at 7 mph. Tests included two (test-retest) progressive, step increment (2 1/2% grade elevation), discontinuous tests (DCT); a progressive, step increment, continuous test (ct) and two constant load tests (CL and CL +2 1/2%). A DCT test was performed first for establishment of peak elevation levels as constant load tests were performed at the peak elevation level attained (CL) and at a level 2 1/2% higher (CL + 2 1/2%). The second DCT test and the remaining three tests were administered randomly. Peak preformance capability (operationally defined as duration at highest grade elevation) was markedly reduced during progressive tests as compared with constant load tests. There was a similar reduction in peak performance capability during the CT test as compared with DCT test. Maximal oxygen uptake (VO2max) was quite similar among the various tests. It was concluded that VO2max attained during progressive, step increment, tests is unaffected by cumulative submaximal work. Discontinuous and continuous progressive tests provide similar VO2max results.", "contents": "Step increment versus constant load tests for determination of maximal oxygen uptake. Ten male subjects performed five maximal treadmill running tests at 7 mph. Tests included two (test-retest) progressive, step increment (2 1/2% grade elevation), discontinuous tests (DCT); a progressive, step increment, continuous test (ct) and two constant load tests (CL and CL +2 1/2%). A DCT test was performed first for establishment of peak elevation levels as constant load tests were performed at the peak elevation level attained (CL) and at a level 2 1/2% higher (CL + 2 1/2%). The second DCT test and the remaining three tests were administered randomly. Peak preformance capability (operationally defined as duration at highest grade elevation) was markedly reduced during progressive tests as compared with constant load tests. There was a similar reduction in peak performance capability during the CT test as compared with DCT test. Maximal oxygen uptake (VO2max) was quite similar among the various tests. It was concluded that VO2max attained during progressive, step increment, tests is unaffected by cumulative submaximal work. Discontinuous and continuous progressive tests provide similar VO2max results.", "PMID": 954734}
{"id": "pubmed23n0032_1292", "title": "The effect of various levels of strenuous to exhaustive exercise on reaction time.", "content": "Reaction times and heart rates of 10 subjects walking and running on a motor-driven treadmill were recorded. The exercise consisted of four 3-min stages: Stage 1, 2.5 mph at 12% grade; Stage 2, 3.4 mpg at 14% grade; Stage 3, 4.2 mph at 16% grade; Stage 4 5.0 mph at 18% grade. Subjects were given three separate tests at 1-week intervals. There was an increase in reaction time with increased exercise, but this effect diminished significantly over days. Also, when heart rate was 80% of maximum during the post-test, reaction time was not significantly different from the pre-test. Optimum reaction time was obtained at approximately 40% of maximum heart rate.", "contents": "The effect of various levels of strenuous to exhaustive exercise on reaction time. Reaction times and heart rates of 10 subjects walking and running on a motor-driven treadmill were recorded. The exercise consisted of four 3-min stages: Stage 1, 2.5 mph at 12% grade; Stage 2, 3.4 mpg at 14% grade; Stage 3, 4.2 mph at 16% grade; Stage 4 5.0 mph at 18% grade. Subjects were given three separate tests at 1-week intervals. There was an increase in reaction time with increased exercise, but this effect diminished significantly over days. Also, when heart rate was 80% of maximum during the post-test, reaction time was not significantly different from the pre-test. Optimum reaction time was obtained at approximately 40% of maximum heart rate.", "PMID": 954735}
{"id": "pubmed23n0032_1293", "title": "The aerobic power of several groups of laborers in Colombia and the United States.", "content": "Physiological responses to exercise and anthropometry were measured in several groups of physical laborers. The groups included Colombian sugar cane cutters, sugar cane loaders and general farm laborers. In addition, migrant workers and civil service laborers from the United States were studied. The Colombian populations were generally shorter, weighed less and had less body fat than the United States group. The nutritional state of the Colombian groups, as measured by the hematocrit, hemoglobin, serum albumin and total blood protein, appeared normal. The aerobic power of the Colombia populations was significantly larger than the United States groups. The larger aerobic power of the Colombian groups is probably related to greater physical fitness associated with the physical demands of their occupations.", "contents": "The aerobic power of several groups of laborers in Colombia and the United States. Physiological responses to exercise and anthropometry were measured in several groups of physical laborers. The groups included Colombian sugar cane cutters, sugar cane loaders and general farm laborers. In addition, migrant workers and civil service laborers from the United States were studied. The Colombian populations were generally shorter, weighed less and had less body fat than the United States group. The nutritional state of the Colombian groups, as measured by the hematocrit, hemoglobin, serum albumin and total blood protein, appeared normal. The aerobic power of the Colombia populations was significantly larger than the United States groups. The larger aerobic power of the Colombian groups is probably related to greater physical fitness associated with the physical demands of their occupations.", "PMID": 954736}
{"id": "pubmed23n0032_1294", "title": "Functional significance of collateral blood flow in working skeletal muscle of the dog.", "content": "The semitendinosus muscle of the dog is supplied by two separate arteries and drained by two corresponding veins. In the muscles used in this study no blood entering via the distal artery was found to leave via the proximal vein during perfusion through both arteries (orthograde perfusion). Therefore, collateral flow (CF) could be determined as proximal venous outflow during occlusion of the proximal artery. During orthograde perfusion total blood flow averaged 12 ml x min-1 x 100 g-1 at rest and 58.4 ml x min-1 x 100 g-1 during exercise. CF was found to average 6.2 ml x min-1 x 100 g-1 at rest and increased to 9.2 ml x min-1 x 100 g-1 during exercise. CF was sufficient to cover the metabolic demand of resting muscle. During exercise the O2-uptake (VO2) of the distal muscle portion was increased 13.4 fold in comparison to a 3.1 fold increase in the proximal muscle portion. The average contractile power decreased by 46%. Additional infusion of adenosine into the distal artery resulted in an increase of CF to 11.4 ml x min-1 x 100 g-1 and of orthograde flow to 71 ml x min-1 x 100 g-1. The average contractile power of the muscle increased by 13%. Both orthograde flow and CF were found to decrease with increasing muscle load. But this decrease was significantly more pronounced in the case of CF especially at a lower range of loads. It is concluded that after acute occlusion of orthograde flow, CF is limited by the number, the size and the dilatory capacity of precapillary network vessels. Furthermore, CF is influenced considerably by changes of extravascular support.", "contents": "Functional significance of collateral blood flow in working skeletal muscle of the dog. The semitendinosus muscle of the dog is supplied by two separate arteries and drained by two corresponding veins. In the muscles used in this study no blood entering via the distal artery was found to leave via the proximal vein during perfusion through both arteries (orthograde perfusion). Therefore, collateral flow (CF) could be determined as proximal venous outflow during occlusion of the proximal artery. During orthograde perfusion total blood flow averaged 12 ml x min-1 x 100 g-1 at rest and 58.4 ml x min-1 x 100 g-1 during exercise. CF was found to average 6.2 ml x min-1 x 100 g-1 at rest and increased to 9.2 ml x min-1 x 100 g-1 during exercise. CF was sufficient to cover the metabolic demand of resting muscle. During exercise the O2-uptake (VO2) of the distal muscle portion was increased 13.4 fold in comparison to a 3.1 fold increase in the proximal muscle portion. The average contractile power decreased by 46%. Additional infusion of adenosine into the distal artery resulted in an increase of CF to 11.4 ml x min-1 x 100 g-1 and of orthograde flow to 71 ml x min-1 x 100 g-1. The average contractile power of the muscle increased by 13%. Both orthograde flow and CF were found to decrease with increasing muscle load. But this decrease was significantly more pronounced in the case of CF especially at a lower range of loads. It is concluded that after acute occlusion of orthograde flow, CF is limited by the number, the size and the dilatory capacity of precapillary network vessels. Furthermore, CF is influenced considerably by changes of extravascular support.", "PMID": 954737}
{"id": "pubmed23n0032_1295", "title": "A comparison of maximum oxygen uptake determination by bicycle ergometry at various pedaling frequencies and by treadmill running at various speeds.", "content": "Tests of performance on a bicycle ergometer have routinely been standardized with pedaling frequencies of up to 60 rpm. Only rarely have higher speeds been used. It may be hypothesized than a higher VO2, more closely approximating the maximum attainable by treadmill running, may be achieved in bicycle ergometry if higher pedal frequencies are used so that premature fatigue of the leg musculature does not obscure the comparison. Even in treadmill running it remains equivocal which combination of speed and grade of running will produce a maximum VO2. Five male subjects performed maximally at pedal frequencies of 60, 80, 100 and 120 rpm and running speeds of 6.0, 6.5, 7.0, and 7.5 mph, on a bicycle and treadmill respectively. Power output on the bicycle and increasing grade sequence on the treadmill were maintained constant for each speed investigated. The highest VO2 attained in each test was measured and compared both within and between the modes of testing. It was confirmed that peak VO2 during bicycle ergometry is significantly less than VO2 max attained in treadmill running. Pedal frequencies of 80 and 100 rpm produced optimal VO2's in ergometry. No differences were noted between VO2's determined at any speed of treadmill running. Subjects preferred 60 or 80 rpm in ergometry and 6.0 and 7.0 mph in treadmill running; 120 rpm and 7.5 mph in bicycling and treadmill running, respectively, were generally disliked.", "contents": "A comparison of maximum oxygen uptake determination by bicycle ergometry at various pedaling frequencies and by treadmill running at various speeds. Tests of performance on a bicycle ergometer have routinely been standardized with pedaling frequencies of up to 60 rpm. Only rarely have higher speeds been used. It may be hypothesized than a higher VO2, more closely approximating the maximum attainable by treadmill running, may be achieved in bicycle ergometry if higher pedal frequencies are used so that premature fatigue of the leg musculature does not obscure the comparison. Even in treadmill running it remains equivocal which combination of speed and grade of running will produce a maximum VO2. Five male subjects performed maximally at pedal frequencies of 60, 80, 100 and 120 rpm and running speeds of 6.0, 6.5, 7.0, and 7.5 mph, on a bicycle and treadmill respectively. Power output on the bicycle and increasing grade sequence on the treadmill were maintained constant for each speed investigated. The highest VO2 attained in each test was measured and compared both within and between the modes of testing. It was confirmed that peak VO2 during bicycle ergometry is significantly less than VO2 max attained in treadmill running. Pedal frequencies of 80 and 100 rpm produced optimal VO2's in ergometry. No differences were noted between VO2's determined at any speed of treadmill running. Subjects preferred 60 or 80 rpm in ergometry and 6.0 and 7.0 mph in treadmill running; 120 rpm and 7.5 mph in bicycling and treadmill running, respectively, were generally disliked.", "PMID": 954738}
{"id": "pubmed23n0032_1296", "title": "Muscle-pO2 in trained and untrained non-anesthetized guinea pigs and in men.", "content": "pO2-measurements were performed by micro-Pt-electrodes a) in the gastrocnemius muscles of physically trained and untrained non-anesthetized guinea pigs, and b) in the tibialis anterior muscles of physically trained and untrained men, and of patients after a 2-3 weeks bedrest. Physical training raises the mean pO2-values in the resulting muscles of guinea pigs and men from 30-40 Torr to 50-55 Torr whereas physical inactivity (2-3 weeks bedrest) reduces the values to 17-18 Torr.", "contents": "Muscle-pO2 in trained and untrained non-anesthetized guinea pigs and in men. pO2-measurements were performed by micro-Pt-electrodes a) in the gastrocnemius muscles of physically trained and untrained non-anesthetized guinea pigs, and b) in the tibialis anterior muscles of physically trained and untrained men, and of patients after a 2-3 weeks bedrest. Physical training raises the mean pO2-values in the resulting muscles of guinea pigs and men from 30-40 Torr to 50-55 Torr whereas physical inactivity (2-3 weeks bedrest) reduces the values to 17-18 Torr.", "PMID": 954739}
{"id": "pubmed23n0032_1297", "title": "A new formula for estimating metabolic rate.", "content": "A formula for estimating metaoblic rate using inlet volume flow and oxygen fractionation of outlet air is derived. The formula is simplified and the error induced is evaluated and compared with that of Weir's formula.", "contents": "A new formula for estimating metabolic rate. A formula for estimating metaoblic rate using inlet volume flow and oxygen fractionation of outlet air is derived. The formula is simplified and the error induced is evaluated and compared with that of Weir's formula.", "PMID": 954740}
{"id": "pubmed23n0032_1298", "title": "Reaction of N-(3-pyrene)maleimide with thiol groups of reticulocyte ribosomes.", "content": "The reaction of N-(3-pyrene)maleimide with thiol groups of rabbit reticulocyte ribosomes offers a possible fluorescent probe for studying ribosomal structure and conformation. At relatively low concentrations of N-(3-pyrene)maleimide a group of 30-40 readily reactive sulfhydryl residues is derivatized. The major ribosomal proteins containing these thiol groups are identified as S2 + S3, S5, S7, S8, S29, S31, S32, L1, L5, L6, L10 + L14, L15, L18 + L19, and L36. Ribosomal activity, as measured by the nonenzymic binding of phenylalanyl-tRNA and polyphenylalanine synthesis, is inhibited by this degree of reaction with N-(3-pyrene)maleimide. The inhibition is relieved by the prior binding of polyuridylic acid to the ribosomes while the extent of derivatization by N-(3-pyrene)-maleimide is diminished only slightly. The average relative polarization of the fluorescence of the ribosomal bound N-(3-pyrene)maleimide changes significantly with the degree of derivatization of ribosomal thiol groups or with the binding of polyuridylic acid, indicating the value of such a fluorescent thiol-derivatizing agent as a probe of ribosomal structure.", "contents": "Reaction of N-(3-pyrene)maleimide with thiol groups of reticulocyte ribosomes. The reaction of N-(3-pyrene)maleimide with thiol groups of rabbit reticulocyte ribosomes offers a possible fluorescent probe for studying ribosomal structure and conformation. At relatively low concentrations of N-(3-pyrene)maleimide a group of 30-40 readily reactive sulfhydryl residues is derivatized. The major ribosomal proteins containing these thiol groups are identified as S2 + S3, S5, S7, S8, S29, S31, S32, L1, L5, L6, L10 + L14, L15, L18 + L19, and L36. Ribosomal activity, as measured by the nonenzymic binding of phenylalanyl-tRNA and polyphenylalanine synthesis, is inhibited by this degree of reaction with N-(3-pyrene)maleimide. The inhibition is relieved by the prior binding of polyuridylic acid to the ribosomes while the extent of derivatization by N-(3-pyrene)-maleimide is diminished only slightly. The average relative polarization of the fluorescence of the ribosomal bound N-(3-pyrene)maleimide changes significantly with the degree of derivatization of ribosomal thiol groups or with the binding of polyuridylic acid, indicating the value of such a fluorescent thiol-derivatizing agent as a probe of ribosomal structure.", "PMID": 954741}
{"id": "pubmed23n0032_1299", "title": "[Circular dichroism of heterodetic cyclic cystinyl tripeptides (author's transl)].", "content": "The circular dichroism of two series of heterodetic cyclic tripeptides, t-butoxycarbonyl-L-cysteinyl-X-cysteine disulfide methyl esters [X=Ala, Val, Leu, Phe, Glu(OBut), Lys(Z)] and L-cysteinyl-X-cysteine disulfide methyl ester formates, and a corresponding series of t-butoxycarbonyl-L-cysteinyl-(glycyl)n-cysteine disulfide methyl esters (n=1-5) was investigated. The interaction between the disulfide group and the amide bonds results in a long-wavelength Cotton effect at 300 nm, which exhibits a strong solvent dependence. In contrast to the long-wavelength disulfide band and to the n-pi amide Cotton effect a bathochromic shift is observed from less polar to strongly polar solvents. Within the series of N-terminal protected tripeptide esters the influence of the central chiral amino acid X is small, but it increases in the non-protected peptide esters. The circular dichroism is dominated by the influence of the conformation of the disulfide ring, which is essentially the same in both series. Due to interactions of the disulfide group with the amide chromophores, the sign of the long-wavelength disulfide Cotton effect cannot be used for the determination of the helicity of the CSSC group. Infrared spectra of the protected compounds indicate trans-peptide bonds in the solid state, which may also be true for the solutions. A pronounced time-dependence is observed for the circular dichroism of t-butoxycarbonyl-L-cysteinyl-glycyl-cysteine disulfide methyl ester after dissolving in ethanol. This is due to a uniform conformational change of the ring structure as shown by spectroscopic analysis using extinction and ellipticity diagrams. In the series of the cyclic cystinyl peptides containing glycyl residues a strong electronic disulfide-amide interaction is observed is observed for the tripeptide, being still observable for the tetra-peptide. The comparison of the glycyl tripeptide with the tripeptides containing chiral central amino acids reveals a negative contribution to the circular dichroism at 260 nm due to these L-residues.", "contents": "[Circular dichroism of heterodetic cyclic cystinyl tripeptides (author's transl)]. The circular dichroism of two series of heterodetic cyclic tripeptides, t-butoxycarbonyl-L-cysteinyl-X-cysteine disulfide methyl esters [X=Ala, Val, Leu, Phe, Glu(OBut), Lys(Z)] and L-cysteinyl-X-cysteine disulfide methyl ester formates, and a corresponding series of t-butoxycarbonyl-L-cysteinyl-(glycyl)n-cysteine disulfide methyl esters (n=1-5) was investigated. The interaction between the disulfide group and the amide bonds results in a long-wavelength Cotton effect at 300 nm, which exhibits a strong solvent dependence. In contrast to the long-wavelength disulfide band and to the n-pi amide Cotton effect a bathochromic shift is observed from less polar to strongly polar solvents. Within the series of N-terminal protected tripeptide esters the influence of the central chiral amino acid X is small, but it increases in the non-protected peptide esters. The circular dichroism is dominated by the influence of the conformation of the disulfide ring, which is essentially the same in both series. Due to interactions of the disulfide group with the amide chromophores, the sign of the long-wavelength disulfide Cotton effect cannot be used for the determination of the helicity of the CSSC group. Infrared spectra of the protected compounds indicate trans-peptide bonds in the solid state, which may also be true for the solutions. A pronounced time-dependence is observed for the circular dichroism of t-butoxycarbonyl-L-cysteinyl-glycyl-cysteine disulfide methyl ester after dissolving in ethanol. This is due to a uniform conformational change of the ring structure as shown by spectroscopic analysis using extinction and ellipticity diagrams. In the series of the cyclic cystinyl peptides containing glycyl residues a strong electronic disulfide-amide interaction is observed is observed for the tripeptide, being still observable for the tetra-peptide. The comparison of the glycyl tripeptide with the tripeptides containing chiral central amino acids reveals a negative contribution to the circular dichroism at 260 nm due to these L-residues.", "PMID": 954742}
{"id": "pubmed23n0032_1300", "title": "The enthalpy of interaction of urea with some globular proteins.", "content": "The enthalpies of interaction of urea with five globular proteins, ribonuclease A, trypsin, beta-lacto-globulin, ovalbumin and bovine serum albumin have been measured in aqueous solution at pH 7.0, I=0.005 M and 25 degrees C over a range of urea molality m from 0-15 mmol g-1 (where a 1 molal solution contains 1 mmol g-1). For all the proteins the interaction is exothermic, and there is an appreciable heat evolution at low urea concentrations, m less than 5 mmol g-1, which increases sharply at higher urea concentrations when the proteins undergo unfolding. If account is taken of the endothermic enthalpies of unfolding of the native proteins, the enthalpies of interactions of urea per unit mass denatured protein lie in the range -45 to -75 J g-1, corresponding to an average binding enthalpy of -23 kJ mol-1 bound urea.", "contents": "The enthalpy of interaction of urea with some globular proteins. The enthalpies of interaction of urea with five globular proteins, ribonuclease A, trypsin, beta-lacto-globulin, ovalbumin and bovine serum albumin have been measured in aqueous solution at pH 7.0, I=0.005 M and 25 degrees C over a range of urea molality m from 0-15 mmol g-1 (where a 1 molal solution contains 1 mmol g-1). For all the proteins the interaction is exothermic, and there is an appreciable heat evolution at low urea concentrations, m less than 5 mmol g-1, which increases sharply at higher urea concentrations when the proteins undergo unfolding. If account is taken of the endothermic enthalpies of unfolding of the native proteins, the enthalpies of interactions of urea per unit mass denatured protein lie in the range -45 to -75 J g-1, corresponding to an average binding enthalpy of -23 kJ mol-1 bound urea.", "PMID": 954743}
{"id": "pubmed23n0032_1301", "title": "Investigation of the covalent bond structure of peptide-matrix systems by Edman degradation of support-fixed peptides.", "content": "Two model peptides Ala-Ser-Asp-Tyr-Leu and Ala-Ser-Glu-Tyr-Leu have been synthesized and coupled with an without protecting groups to the amino groups of polystyrene resins. The yield in the binding operation was 93-97% by using water-soluble carbodiimides as condensing reagents. Edman degradation of the products and quantitative estimation of the amino acid phenylthio-hydantoins, as well as amino acid analysis of the partially and completely degraded resins, showed about 7-10% of the fixed peptides not to be involved in the degradation procedure. That part of the material which was not involved in the Edman degradation during the first step also remained inactive in the following degradation operations. The analytical data presented in the paper explain not only the course of the Edman degradation of peptides linked via the side-chain carboxylic groups to the resin, but they also describe the chemical reactivity of the various carboxylic groups in the binding operation. 22% of the peptides are bound to the resin by side-chain carboxylic groups in the case of the aspartate-containing peptide, 26-27% in the case of the glutamate-containing peptide. 75-77% and 72-75% respectively of the peptides are fixed to the resin via the C-terminal carboxylic group. No more than 3% of the fixed material may be bound bifunctionally to the support.", "contents": "Investigation of the covalent bond structure of peptide-matrix systems by Edman degradation of support-fixed peptides. Two model peptides Ala-Ser-Asp-Tyr-Leu and Ala-Ser-Glu-Tyr-Leu have been synthesized and coupled with an without protecting groups to the amino groups of polystyrene resins. The yield in the binding operation was 93-97% by using water-soluble carbodiimides as condensing reagents. Edman degradation of the products and quantitative estimation of the amino acid phenylthio-hydantoins, as well as amino acid analysis of the partially and completely degraded resins, showed about 7-10% of the fixed peptides not to be involved in the degradation procedure. That part of the material which was not involved in the Edman degradation during the first step also remained inactive in the following degradation operations. The analytical data presented in the paper explain not only the course of the Edman degradation of peptides linked via the side-chain carboxylic groups to the resin, but they also describe the chemical reactivity of the various carboxylic groups in the binding operation. 22% of the peptides are bound to the resin by side-chain carboxylic groups in the case of the aspartate-containing peptide, 26-27% in the case of the glutamate-containing peptide. 75-77% and 72-75% respectively of the peptides are fixed to the resin via the C-terminal carboxylic group. No more than 3% of the fixed material may be bound bifunctionally to the support.", "PMID": 954744}
{"id": "pubmed23n0032_1302", "title": "Light chains from slow-twitch muscle myosin.", "content": "Myosin light chains have been isolated from slow-twitch soleus muscles of rabbit and cat. Two chemically related light chains of molecular weight about 22 000 have been identified from their thiol sequences in each species, and these have been further characterized by amino acid analysis and peptide mapping studies. These light chains are related to the alkali light chains of rabbit fast-twitch muscles and to the larger cardiac light chain from bovine heart muscle. The presence of two chemically related but phenotypically distinct light chains within single muscles suggests the presence of myosin isoenzyme or that the myosin molecule has a different light chain associated with each subfragment-1 head. Although the stoichiometry of these two light chains had not been determined, the former of these two conclusions is favoured by analogy with experiments on fast-twitch myosins. In addition to these related light chains, soleus muscle myosin, like fast-twitch myosins, contains a third light chain of about 19 000 molecular weight. Unlike the corresponding light chain of rabbit fast-twitch myosin, this 19 000-Mr light chain contrains no cysteine residues. The distribution of thiol peptides together with the characteristic mobilities of these light chains on polyacrylamide gels in the presence of sodium dodecyl sulphate provides a 'fingerprint' of myosins from different muscle types. This has been used to look for the presence of fast-twitch myosin in soleus muscle, the results showing both rabbit and cat soleus muscles are homogeneous in their myosin type within the sensitivity of detection of the methods. These techniques have also been used to confirm the reciprocal transformation of light chains in myosins isolated from cross-reinnervated muscles. Finally the relationships between these criteria for different myosin types and histochemical procedures for muscle fibre typing are discussed.", "contents": "Light chains from slow-twitch muscle myosin. Myosin light chains have been isolated from slow-twitch soleus muscles of rabbit and cat. Two chemically related light chains of molecular weight about 22 000 have been identified from their thiol sequences in each species, and these have been further characterized by amino acid analysis and peptide mapping studies. These light chains are related to the alkali light chains of rabbit fast-twitch muscles and to the larger cardiac light chain from bovine heart muscle. The presence of two chemically related but phenotypically distinct light chains within single muscles suggests the presence of myosin isoenzyme or that the myosin molecule has a different light chain associated with each subfragment-1 head. Although the stoichiometry of these two light chains had not been determined, the former of these two conclusions is favoured by analogy with experiments on fast-twitch myosins. In addition to these related light chains, soleus muscle myosin, like fast-twitch myosins, contains a third light chain of about 19 000 molecular weight. Unlike the corresponding light chain of rabbit fast-twitch myosin, this 19 000-Mr light chain contrains no cysteine residues. The distribution of thiol peptides together with the characteristic mobilities of these light chains on polyacrylamide gels in the presence of sodium dodecyl sulphate provides a 'fingerprint' of myosins from different muscle types. This has been used to look for the presence of fast-twitch myosin in soleus muscle, the results showing both rabbit and cat soleus muscles are homogeneous in their myosin type within the sensitivity of detection of the methods. These techniques have also been used to confirm the reciprocal transformation of light chains in myosins isolated from cross-reinnervated muscles. Finally the relationships between these criteria for different myosin types and histochemical procedures for muscle fibre typing are discussed.", "PMID": 954745}
{"id": "pubmed23n0032_1303", "title": "Enhancement of hydroxyindole-O-methyltransferase and DNA-dependent RNA polymerase activities induced by oestradiol in rat pineals in culture.", "content": "1. Hydroxyindole-O-methyltransferase and DNA-dependent RNA polymerase activities were determined in the pineal gland removed from the ovariectomised rat and cultured under various experimental conditions. 2. The transferase activity declined very slowly during 24 h of incubation. 17beta-Oestradiol significantly increased the transferase activity within 2h after the addition, and the extent of increase was dose-dependent within the concentration range from 0.1 to 15 nM, being increased by 80% at 15 nM. Enhancement of the transferase activity by oestradiol was abolished not only by inhibitors of protein synthesis (cycloheximide and puromycin), but also by those of RNA synthesis (actinomycin D and alpha-amanitin). It was also blocked by clomiphene citrate, an agent which is known to inhibit the binding of steroid hormones to their respective receptors. 3. RNA polymerase activity (forms A and B) declined rapidly during the initial period of pineal culture. Oestradiol (15 nM) increased the RNA polymerase B activity by 50% within 2 h after the addition. The increase was dose-dependent within the concentration range from 0.1 to 15 nM, and was abolished by clomiphene citrate. 4. The possibility is suggested that the pineal is a target organ of oestradiol, and that the steroid-induced reaction sequence in the pineal conforms to that is known in other target organs.", "contents": "Enhancement of hydroxyindole-O-methyltransferase and DNA-dependent RNA polymerase activities induced by oestradiol in rat pineals in culture. 1. Hydroxyindole-O-methyltransferase and DNA-dependent RNA polymerase activities were determined in the pineal gland removed from the ovariectomised rat and cultured under various experimental conditions. 2. The transferase activity declined very slowly during 24 h of incubation. 17beta-Oestradiol significantly increased the transferase activity within 2h after the addition, and the extent of increase was dose-dependent within the concentration range from 0.1 to 15 nM, being increased by 80% at 15 nM. Enhancement of the transferase activity by oestradiol was abolished not only by inhibitors of protein synthesis (cycloheximide and puromycin), but also by those of RNA synthesis (actinomycin D and alpha-amanitin). It was also blocked by clomiphene citrate, an agent which is known to inhibit the binding of steroid hormones to their respective receptors. 3. RNA polymerase activity (forms A and B) declined rapidly during the initial period of pineal culture. Oestradiol (15 nM) increased the RNA polymerase B activity by 50% within 2 h after the addition. The increase was dose-dependent within the concentration range from 0.1 to 15 nM, and was abolished by clomiphene citrate. 4. The possibility is suggested that the pineal is a target organ of oestradiol, and that the steroid-induced reaction sequence in the pineal conforms to that is known in other target organs.", "PMID": 954746}
{"id": "pubmed23n0032_1304", "title": "Study of the interaction of 4-bromomercuriocinnamic acid with alpha-chymotrypsin by 79 Br and 81Br pulsed nuclear-magnetic resonance.", "content": "Br- has been used as a nuclear magnetic resonance (NMR) probe to study the reversible association of alpha-chymotrypsin and an Hg-labelled substrate (4-bromomercuriocinnamic acid, BrHgCin) which rapidly exchanges Br-. T1 was measured for 79Br and 81Br, using a pulse spectrometer. Values of the parameters that determine T1, Obs in aqueous solutions of KBr (pH=5.5) containing alpha-chymotrypsin and BrHgCin are reported. It is found that the rate of Br exchange is diffusion-limited and faster than the rate of reorientation of the BrHgCin-alpha-chymotrypsin complex. The rate constant for the formation of the covalent BrHgCin-alpha-chymotrypsin complex determined by this technique agrees well with previously published data. The rapid rate of Br exchange with the complex, however, is incompatible with the side chain of BrHgCin being entirely buried in a nonpolar pocket on the enzyme but compatible with the side chain being exposed to the solution. The contribution to the NMR signal from the non-covalent complex is negligible.", "contents": "Study of the interaction of 4-bromomercuriocinnamic acid with alpha-chymotrypsin by 79 Br and 81Br pulsed nuclear-magnetic resonance. Br- has been used as a nuclear magnetic resonance (NMR) probe to study the reversible association of alpha-chymotrypsin and an Hg-labelled substrate (4-bromomercuriocinnamic acid, BrHgCin) which rapidly exchanges Br-. T1 was measured for 79Br and 81Br, using a pulse spectrometer. Values of the parameters that determine T1, Obs in aqueous solutions of KBr (pH=5.5) containing alpha-chymotrypsin and BrHgCin are reported. It is found that the rate of Br exchange is diffusion-limited and faster than the rate of reorientation of the BrHgCin-alpha-chymotrypsin complex. The rate constant for the formation of the covalent BrHgCin-alpha-chymotrypsin complex determined by this technique agrees well with previously published data. The rapid rate of Br exchange with the complex, however, is incompatible with the side chain of BrHgCin being entirely buried in a nonpolar pocket on the enzyme but compatible with the side chain being exposed to the solution. The contribution to the NMR signal from the non-covalent complex is negligible.", "PMID": 954747}
{"id": "pubmed23n0032_1305", "title": "Studies on the structure and function of chick-oviduct chromatin. 1. Fractionation by ECTHAM-cellulose chromatography and physico-chemical characterization.", "content": "Chick oviduct chromatin was separated into a ribonucleoprotein fraction and two chromatin fractions (early and late eluting). We utilized a gentle procedure in which moderately hydrated chromatin was subjected to chromatography on a weak ionic-exchange resin (ECTHAM-cellulose) eluted with a combined pH-salt gradient. Chemical analysis of the early (fraction I) and late (fraction II) eluting fractions revealed that their histones were identical and their nonhistone proteins were markedly different. Control experiments showed that these differences were not due to protein rearrangements during chromatin preparation and/or fractionation. The physical properties of fraction I and II differed in certain aspects. The aggregation response of fraction I to increasing concentrations of monovalent cations was five times lower than that of fraction II but the aggregation response to divalent cations was identical. Thermal denaturation assays of DNAs isolated from fractions I and II revealed identical derivative profiles of hyperchromicity vs temperature, thereby indicating similar base composition in the two fractions. Circular dichroism, spectra of the purified DNAs isolated from both fractions showed identical B-type conformations. However, DNA renaturation kinetics analyzed by computer technique indicated that fraction I DNA contained less than half the amount of highly repetitive sequences as compared to either unfractionated chromatin or fraction II. Circular dichroism spectra of fraction I and II chromatins (at room temperature) showed significant differences in a wavelength region were only DNA is optically active (i.e. 255-320 nm). These results indicated that the DNA complexed to proteins in fraction II assumed a more C-type conformation than the DNA in fraction I. The differences in the circular dichroism spectra could not be accounted for by differences in the RNAs or protein chromophores contained in fraction I and fraction II. When the circular dichroism spectra of fraction I and II were recorded at 55 degrees C, the differences between the two fractions were abolished. These results were interpreted to mean that the differences in the DNA conformations found in fractions I and II were due to the differences in their nonhistone proteins. These proteins were effective in maintaining DNA conformation differences only when they were in their native form but not when heated to 55 degree C. Comparison of the sedimentation coefficients of fractions I and II with their calculated molecular weights suggested a more extended structure in fraction I as compared to a more compact structure in fraction II. Only small differences were observed between fraction I and fraction II with respect to either buoyant density analysis in a metrizamide gradient or in the number of phosphate charges accessible to polylysine.", "contents": "Studies on the structure and function of chick-oviduct chromatin. 1. Fractionation by ECTHAM-cellulose chromatography and physico-chemical characterization. Chick oviduct chromatin was separated into a ribonucleoprotein fraction and two chromatin fractions (early and late eluting). We utilized a gentle procedure in which moderately hydrated chromatin was subjected to chromatography on a weak ionic-exchange resin (ECTHAM-cellulose) eluted with a combined pH-salt gradient. Chemical analysis of the early (fraction I) and late (fraction II) eluting fractions revealed that their histones were identical and their nonhistone proteins were markedly different. Control experiments showed that these differences were not due to protein rearrangements during chromatin preparation and/or fractionation. The physical properties of fraction I and II differed in certain aspects. The aggregation response of fraction I to increasing concentrations of monovalent cations was five times lower than that of fraction II but the aggregation response to divalent cations was identical. Thermal denaturation assays of DNAs isolated from fractions I and II revealed identical derivative profiles of hyperchromicity vs temperature, thereby indicating similar base composition in the two fractions. Circular dichroism, spectra of the purified DNAs isolated from both fractions showed identical B-type conformations. However, DNA renaturation kinetics analyzed by computer technique indicated that fraction I DNA contained less than half the amount of highly repetitive sequences as compared to either unfractionated chromatin or fraction II. Circular dichroism spectra of fraction I and II chromatins (at room temperature) showed significant differences in a wavelength region were only DNA is optically active (i.e. 255-320 nm). These results indicated that the DNA complexed to proteins in fraction II assumed a more C-type conformation than the DNA in fraction I. The differences in the circular dichroism spectra could not be accounted for by differences in the RNAs or protein chromophores contained in fraction I and fraction II. When the circular dichroism spectra of fraction I and II were recorded at 55 degrees C, the differences between the two fractions were abolished. These results were interpreted to mean that the differences in the DNA conformations found in fractions I and II were due to the differences in their nonhistone proteins. These proteins were effective in maintaining DNA conformation differences only when they were in their native form but not when heated to 55 degree C. Comparison of the sedimentation coefficients of fractions I and II with their calculated molecular weights suggested a more extended structure in fraction I as compared to a more compact structure in fraction II. Only small differences were observed between fraction I and fraction II with respect to either buoyant density analysis in a metrizamide gradient or in the number of phosphate charges accessible to polylysine.", "PMID": 954749}
{"id": "pubmed23n0032_1306", "title": "Isolation and characterization of iron-binding proteins from rat intestinal mucosa.", "content": "Two iron-binding proteins were isolated from rat intestinal mucosa. From determination of their molecular weights, their electrophoretic and iron-binding properties it was established that one was a mucosal ferritin and the other a mucosal transferrin. The mucosal ferritin is compared in its molecular weight, isoelectric point, amino acid composition and tryptic peptide pattern with the ferritins of rat spleen and liver. All three ferritins are distinctly different from one another. In addition the iron content of mucosal ferritin was found to be much lower than that of liver and spleen ferritins. Mucosal transferrin was separated into two components by isoelectric focusing, as was plasma transferrin. The plasma and mucosal transferrins differ in their isoelectric points and in their amino acid compositions. Differences were also found in vitro in the iron-binding of mucosal transferrin as compared with plasma transferrin. The role of these mucosal proteins in the absorption of iron is briefly discussed.", "contents": "Isolation and characterization of iron-binding proteins from rat intestinal mucosa. Two iron-binding proteins were isolated from rat intestinal mucosa. From determination of their molecular weights, their electrophoretic and iron-binding properties it was established that one was a mucosal ferritin and the other a mucosal transferrin. The mucosal ferritin is compared in its molecular weight, isoelectric point, amino acid composition and tryptic peptide pattern with the ferritins of rat spleen and liver. All three ferritins are distinctly different from one another. In addition the iron content of mucosal ferritin was found to be much lower than that of liver and spleen ferritins. Mucosal transferrin was separated into two components by isoelectric focusing, as was plasma transferrin. The plasma and mucosal transferrins differ in their isoelectric points and in their amino acid compositions. Differences were also found in vitro in the iron-binding of mucosal transferrin as compared with plasma transferrin. The role of these mucosal proteins in the absorption of iron is briefly discussed.", "PMID": 954750}
{"id": "pubmed23n0032_1307", "title": "Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.", "content": "Estrogen treatment of Xenopus males leads to the appearance of a new species of poly (A)-containing RNA in the liver, at a stage when large amounts of the estrogen-induced yolk precursor protein, vitellogenin, is produced. This estrogen-induced RNA sediments at 28 S and migrates on gels in aqueous solution with an apparent molecular weight of 2.0 X 10(6). Contour length measurements under denaturing conditions in the electron microscope reveal a molecular weight of 2.34 X 10(6) compared to the mouse 28-S rRNA. Labeling experiments show that the estrogen-induced RNA has a stability than the average liver poly(A)-containing RNA and represents 10-20% of the poly(a)-containing RNA in the cytoplasm after 24 h of labeling. Hybridization of complementary DNA, synthesized on the isolated estrogen-induced RNA, with its template suggests a complexity corresponding to a single species of poly(A)-containing RNA of such a high molecular weight. Hybridization of the complementary DNA with cytoplasmic poly (A)-containing RNA from estrogen-treated Xenopus males and control toads show that the estrogen-induced RNA constitutes 12-15% of all cytoplasmic poly(A)-containing RNA, and is at least 2000-fold less abundant in untreated males. Size, complexity and abundance of the estrogen-induced RNA are characteristics expected for a mRNA coding for vitellogenin.", "contents": "Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen. Estrogen treatment of Xenopus males leads to the appearance of a new species of poly (A)-containing RNA in the liver, at a stage when large amounts of the estrogen-induced yolk precursor protein, vitellogenin, is produced. This estrogen-induced RNA sediments at 28 S and migrates on gels in aqueous solution with an apparent molecular weight of 2.0 X 10(6). Contour length measurements under denaturing conditions in the electron microscope reveal a molecular weight of 2.34 X 10(6) compared to the mouse 28-S rRNA. Labeling experiments show that the estrogen-induced RNA has a stability than the average liver poly(A)-containing RNA and represents 10-20% of the poly(a)-containing RNA in the cytoplasm after 24 h of labeling. Hybridization of complementary DNA, synthesized on the isolated estrogen-induced RNA, with its template suggests a complexity corresponding to a single species of poly(A)-containing RNA of such a high molecular weight. Hybridization of the complementary DNA with cytoplasmic poly (A)-containing RNA from estrogen-treated Xenopus males and control toads show that the estrogen-induced RNA constitutes 12-15% of all cytoplasmic poly(A)-containing RNA, and is at least 2000-fold less abundant in untreated males. Size, complexity and abundance of the estrogen-induced RNA are characteristics expected for a mRNA coding for vitellogenin.", "PMID": 954751}
{"id": "pubmed23n0032_1308", "title": "Intracellular degradation and deamidation of alpha-crystallin subunits.", "content": "The subunites AA2 and N2, present in alpha-crystallin from the nucleus of young and old bovine lenses, were isolated and characterized. It was found that AA2 is identical to a shorter A-chain and hence it was designated as A1-101. The subunit N2 turned out to be identical with a shorter B-chain and was designated as b 1/2-170. Characterization of the subunit N1, present only in alpha-crystallin from the cortex of old bovine lenses, suggested that his subunit is a modified B-chain, probably a deamidation product; it was designated as B0. Con relation to the age of the fiber cells in old bovine lenses with that in calf lenses revealed that the observed specific limited degradation of the subunits of alpha-crystallin increased with older age of the tissue. The deamidation process was found not to be related to the aging of the tissue. Eventually, a clear picture concerning the heterogeneity and fickleness of the alpha-crystallin subunit structure was obtained.", "contents": "Intracellular degradation and deamidation of alpha-crystallin subunits. The subunites AA2 and N2, present in alpha-crystallin from the nucleus of young and old bovine lenses, were isolated and characterized. It was found that AA2 is identical to a shorter A-chain and hence it was designated as A1-101. The subunit N2 turned out to be identical with a shorter B-chain and was designated as b 1/2-170. Characterization of the subunit N1, present only in alpha-crystallin from the cortex of old bovine lenses, suggested that his subunit is a modified B-chain, probably a deamidation product; it was designated as B0. Con relation to the age of the fiber cells in old bovine lenses with that in calf lenses revealed that the observed specific limited degradation of the subunits of alpha-crystallin increased with older age of the tissue. The deamidation process was found not to be related to the aging of the tissue. Eventually, a clear picture concerning the heterogeneity and fickleness of the alpha-crystallin subunit structure was obtained.", "PMID": 954752}
{"id": "pubmed23n0032_1309", "title": "Sulfate bile acids in germ-free and conventional mice.", "content": "Sulfated and non-sulfated bile acids were determined in the intestines and in the feces of 7-month-old germ-free and conventional male mice. 1. The bile acid pools in the gall bladder and small intestine were 21.13 mg/100g body weight in germ-free and 11.50 mg in conventional mice. The bile acid pools in the cecum and large intestine of germ-free mice were 3.03 mg/100 g body weight as compared to 1.24 mg in conventional mice. Fecal bile acid excretion was 2.93 mg and 4.12 mg/100 g body weight in 24 h in germ-free and conventional mice respectively. 2. The major bile acids from germ-free mice were cholic acid, alpha-muricholic acid and beta-muricholic acid. Small amounts of chenodeoxycholic and allocholic acid were also present. In addition to these primary bile acids the following secondary bile acids were identified in conventional mice: lithocholic, deoxycholic and omega-muricholic acid. 3. In both germ-free and conventional animals significant amounts of chenodeoxycholic and cholic acid were present as the 7-monosulfate esters. The sulfate esters of these bile acids did not exceed 2% of the total bile acids in the small intestine, but accounted for approximately 50% of the bile acids in the cecum and the large intestine. In contrast, the muricholic acids were nearly exclusively found in the non-sulfate fraction. 4. Alkaline hydrolysis without prior solvolysis of the sulfate esters resulted in loss of bile acids and production of artifacts. Hence, the bile acids of the mouse cannot be analysed by methods involving alkaline deconjugation unless a solvolysis step is included in the procedure.", "contents": "Sulfate bile acids in germ-free and conventional mice. Sulfated and non-sulfated bile acids were determined in the intestines and in the feces of 7-month-old germ-free and conventional male mice. 1. The bile acid pools in the gall bladder and small intestine were 21.13 mg/100g body weight in germ-free and 11.50 mg in conventional mice. The bile acid pools in the cecum and large intestine of germ-free mice were 3.03 mg/100 g body weight as compared to 1.24 mg in conventional mice. Fecal bile acid excretion was 2.93 mg and 4.12 mg/100 g body weight in 24 h in germ-free and conventional mice respectively. 2. The major bile acids from germ-free mice were cholic acid, alpha-muricholic acid and beta-muricholic acid. Small amounts of chenodeoxycholic and allocholic acid were also present. In addition to these primary bile acids the following secondary bile acids were identified in conventional mice: lithocholic, deoxycholic and omega-muricholic acid. 3. In both germ-free and conventional animals significant amounts of chenodeoxycholic and cholic acid were present as the 7-monosulfate esters. The sulfate esters of these bile acids did not exceed 2% of the total bile acids in the small intestine, but accounted for approximately 50% of the bile acids in the cecum and the large intestine. In contrast, the muricholic acids were nearly exclusively found in the non-sulfate fraction. 4. Alkaline hydrolysis without prior solvolysis of the sulfate esters resulted in loss of bile acids and production of artifacts. Hence, the bile acids of the mouse cannot be analysed by methods involving alkaline deconjugation unless a solvolysis step is included in the procedure.", "PMID": 954753}
{"id": "pubmed23n0032_1310", "title": "Isolation and properties of structured chromatin from Guerin ascites tumour and rat liver.", "content": "The method proposed by Hancock for isolation of structured chromatin from tissue culture cells is modified and used for isolation of chromatin from Guerin ascites tumour and rat liver. Micrococcal nuclease digestion patterns and thermal denaturation of these chromatins are studied and compared wiith those of chromatins prepared by precipitation and extraction with salts (salt chromatins). In contrast to the multiphasic melting profiles and salt chromatins, the structured chromatins exhibit relatively homogeneous denaturation patterns under a variety of conditions, suggesting thhat their DNA is uniformly stabilized by histones and there are no free independently melting DNA stretches. Digestion of structure of the deoxyribonucleoprotein is intact. No discrete fragments are formed upon digestion of salt chromatin.", "contents": "Isolation and properties of structured chromatin from Guerin ascites tumour and rat liver. The method proposed by Hancock for isolation of structured chromatin from tissue culture cells is modified and used for isolation of chromatin from Guerin ascites tumour and rat liver. Micrococcal nuclease digestion patterns and thermal denaturation of these chromatins are studied and compared wiith those of chromatins prepared by precipitation and extraction with salts (salt chromatins). In contrast to the multiphasic melting profiles and salt chromatins, the structured chromatins exhibit relatively homogeneous denaturation patterns under a variety of conditions, suggesting thhat their DNA is uniformly stabilized by histones and there are no free independently melting DNA stretches. Digestion of structure of the deoxyribonucleoprotein is intact. No discrete fragments are formed upon digestion of salt chromatin.", "PMID": 954754}
{"id": "pubmed23n0032_1311", "title": "The use of mercurated nucleoside triphosphate as a probe in transcription studies in vitro.", "content": "1. Purified form A RNA polymerase and the endogenous, nuclear form A RNA polymerase are shown to incorporate 5-mercuri-uridine 5'-triphosphate (Hg-UTP) into RNA in vitro. The Km for both Hg-UTP and UTP are in the region of 10 muM for the purified enzyme. 2. The RNA products formed in nucleoli by endogenous RNA polymerase A have similar base compositions (G + C-rich) whether UTP or Hg-UTP is provided as the substrate in vitro. 3. Sulphydryl-Sepharose chromatography of RNA synthesised in vitro by nucleoli allows separation of this material from the endogenous RNA, when the former is synthesised in the presence of Hg-UTP. 4. In-vitro-synthesised nucleolar RNA hybridises with cot profiles similar to 28-S ribosomal RNA, when made with either Hg-UTP or UTP. 5. Hybridisation studies using DNA excess suggest that little competition occurs between the in vitro transcripts and the endogenous nucleolar RNA. 6. Size analysis of in vitro transcripts show that although some degradation occurs during isolation, purification and hybridisation of the RNA species, most of the RNA remains larger than 5 S throughout.", "contents": "The use of mercurated nucleoside triphosphate as a probe in transcription studies in vitro. 1. Purified form A RNA polymerase and the endogenous, nuclear form A RNA polymerase are shown to incorporate 5-mercuri-uridine 5'-triphosphate (Hg-UTP) into RNA in vitro. The Km for both Hg-UTP and UTP are in the region of 10 muM for the purified enzyme. 2. The RNA products formed in nucleoli by endogenous RNA polymerase A have similar base compositions (G + C-rich) whether UTP or Hg-UTP is provided as the substrate in vitro. 3. Sulphydryl-Sepharose chromatography of RNA synthesised in vitro by nucleoli allows separation of this material from the endogenous RNA, when the former is synthesised in the presence of Hg-UTP. 4. In-vitro-synthesised nucleolar RNA hybridises with cot profiles similar to 28-S ribosomal RNA, when made with either Hg-UTP or UTP. 5. Hybridisation studies using DNA excess suggest that little competition occurs between the in vitro transcripts and the endogenous nucleolar RNA. 6. Size analysis of in vitro transcripts show that although some degradation occurs during isolation, purification and hybridisation of the RNA species, most of the RNA remains larger than 5 S throughout.", "PMID": 954755}
{"id": "pubmed23n0032_1312", "title": "Studies on the formation of acetoin from acetaldehyde by the pyruvate dehydrogenase complex and its regulation.", "content": "1. Pyruvate dehydrogenase complex was isolated from pigeon breast muscle involving steps of isoelectric precipitation, poly(ethyleneglycol) fractionation and separation on a glycerine gradient in the ultracentrifuge. 2. Arsenite, a potent inhibitor of the dihydrolipoyl transcetylase, did not affect the formation of acetoin from acetaldehyde, indicating that the pyruvate dehydrogenase component was operative in this reaction. 3. Production of acetoin by the pyruvate dehydrogenase complex is subject to regulation by phosphorylation and dephosphorylation, the dephosphorylated form only being active. 4. The inhibition by acetaldehyde of the pyruvate dehydrogenase complex could be partly explained by the formation of acetoin as an alternative reaction.", "contents": "Studies on the formation of acetoin from acetaldehyde by the pyruvate dehydrogenase complex and its regulation. 1. Pyruvate dehydrogenase complex was isolated from pigeon breast muscle involving steps of isoelectric precipitation, poly(ethyleneglycol) fractionation and separation on a glycerine gradient in the ultracentrifuge. 2. Arsenite, a potent inhibitor of the dihydrolipoyl transcetylase, did not affect the formation of acetoin from acetaldehyde, indicating that the pyruvate dehydrogenase component was operative in this reaction. 3. Production of acetoin by the pyruvate dehydrogenase complex is subject to regulation by phosphorylation and dephosphorylation, the dephosphorylated form only being active. 4. The inhibition by acetaldehyde of the pyruvate dehydrogenase complex could be partly explained by the formation of acetoin as an alternative reaction.", "PMID": 954756}
{"id": "pubmed23n0032_1313", "title": "Synthesis, separation, identification and interconversion of riboflavin phosphates and their acetyl derivatives: a reinvestigation.", "content": "A reinvestigation of flavin phosphate synthesis, separation, identification, and interconversion was made in view of contradictory results in the literature. It has been confirmed that monochlorophosphoric acid is the best agent for selective 5'-monophosphorylation of riboflavin and derivatives. This reaction yields, however, invariably up to 20% of an isomer, which has been separated by preparative thick-layer chromatography and shown to be the 4'-monophosphate. All the earlier authors failed to detect this isomer which does not bind to flavodoxin. It equilibrates in dilute mineral acid to yield an 8:2 mixture of 5'-phosphate to 4'-phosphate by phosphate migration. The formation of 2',3',4'-triacetyl-flavin mononucleotide, according to Christie, S. M. H., Kenner, G. W. & Todd, A. R. (1954) J. Chem. Soc., 46-52, upon acid-catalysed acetylation of pure FMN, was confirmed. The same reaction under base catalysis, however, does not yield 2',3',4'-triacetyl-flavin mononucleotide as claimed by Khomutova, E. D., Shapiro, T. A., Mezentseva, M. W. & Berezovskii, V. M. (1965) Otd. Obshch. i. Tekhn. Khim., 241-244, Chem. Abstr. 65, 5516a, but in fact up to 80% 2', 3'-diacetyl-flavin 4':5'-cyclophosphate as the main product, which is stable under neutral and weak acidic conditions and does not hydrolyse to 2',3'-diacetyl-flavin 5'-monophosphate as claimed by McCormick, D.B. (1974) J. Heterocycl. Chem. 11, 969-974. The various flavin phosphates and their acetyl derivatives have been identified by pH-titration, electrophoresis, and proton magnetic resonance spectrometry, which direct analyses of crude reaction products as well as a rapid purity check of commerical FMN.", "contents": "Synthesis, separation, identification and interconversion of riboflavin phosphates and their acetyl derivatives: a reinvestigation. A reinvestigation of flavin phosphate synthesis, separation, identification, and interconversion was made in view of contradictory results in the literature. It has been confirmed that monochlorophosphoric acid is the best agent for selective 5'-monophosphorylation of riboflavin and derivatives. This reaction yields, however, invariably up to 20% of an isomer, which has been separated by preparative thick-layer chromatography and shown to be the 4'-monophosphate. All the earlier authors failed to detect this isomer which does not bind to flavodoxin. It equilibrates in dilute mineral acid to yield an 8:2 mixture of 5'-phosphate to 4'-phosphate by phosphate migration. The formation of 2',3',4'-triacetyl-flavin mononucleotide, according to Christie, S. M. H., Kenner, G. W. & Todd, A. R. (1954) J. Chem. Soc., 46-52, upon acid-catalysed acetylation of pure FMN, was confirmed. The same reaction under base catalysis, however, does not yield 2',3',4'-triacetyl-flavin mononucleotide as claimed by Khomutova, E. D., Shapiro, T. A., Mezentseva, M. W. & Berezovskii, V. M. (1965) Otd. Obshch. i. Tekhn. Khim., 241-244, Chem. Abstr. 65, 5516a, but in fact up to 80% 2', 3'-diacetyl-flavin 4':5'-cyclophosphate as the main product, which is stable under neutral and weak acidic conditions and does not hydrolyse to 2',3'-diacetyl-flavin 5'-monophosphate as claimed by McCormick, D.B. (1974) J. Heterocycl. Chem. 11, 969-974. The various flavin phosphates and their acetyl derivatives have been identified by pH-titration, electrophoresis, and proton magnetic resonance spectrometry, which direct analyses of crude reaction products as well as a rapid purity check of commerical FMN.", "PMID": 954757}
{"id": "pubmed23n0032_1314", "title": "Substituent effect on the oxidation of phenols and aromatic amines by horseradish peroxidase compound I.", "content": "A stopped-flow kinetic study shows that the reduction rate of horseradish peroxidase compound I by phenols and aromatic amines is greatly dependent upon the substituent effect on the benzene ring. Morever it has been possible to relate the reduction rate constants of monosubstituted substrates by a linear free-energy relationship (Hammett equation). The correlation of log (rate constants) with sigma values (Hammett equation) and the absence of correlation with sigma+ values (Okamoto-Brown equation) can be explained by a mechanism of aromatic substrate oxidations, in which the substrate gives an electron to the enzyme compound I and simultaneously loses a proton. The analogy which has been made with oxidation potentials of phenols or anilines strengthens the view that the reaction is only dependent on the relative ease of oxidation of the substrate. The rate constant obtained for p-aminophenol indicates that a value of 2.3 X 10(8) M-1 S-1 probably approaches the diffusion-controlled limit for a bimolecular reaction involving compound I and an aromatic substrate.", "contents": "Substituent effect on the oxidation of phenols and aromatic amines by horseradish peroxidase compound I. A stopped-flow kinetic study shows that the reduction rate of horseradish peroxidase compound I by phenols and aromatic amines is greatly dependent upon the substituent effect on the benzene ring. Morever it has been possible to relate the reduction rate constants of monosubstituted substrates by a linear free-energy relationship (Hammett equation). The correlation of log (rate constants) with sigma values (Hammett equation) and the absence of correlation with sigma+ values (Okamoto-Brown equation) can be explained by a mechanism of aromatic substrate oxidations, in which the substrate gives an electron to the enzyme compound I and simultaneously loses a proton. The analogy which has been made with oxidation potentials of phenols or anilines strengthens the view that the reaction is only dependent on the relative ease of oxidation of the substrate. The rate constant obtained for p-aminophenol indicates that a value of 2.3 X 10(8) M-1 S-1 probably approaches the diffusion-controlled limit for a bimolecular reaction involving compound I and an aromatic substrate.", "PMID": 954758}
{"id": "pubmed23n0032_1315", "title": "Therapeutic properties.of haemodialysis and blood exchange transfusion in organophosphate poisoning.", "content": "Human blood was contaminated with nitrostigmine, dimethoate and demeton-S-methyl sulfoxide. It was then dialysed, concentrations of organophosphates were determined and dialysance values calculated. The influence of blood exchange transfusion on poison elimination as well as on the cholinesterase activity of blood, brain and muscle was studied in rats poisoned with nitrostigmine. Haemodialysis was found to be quite an effective method for eliminating demeton-S-methyl sulfoxide and dimethoate, dialysance values of 52.98 ml/min and 59.07 ml/min being found for demeton-S-methyl sulfoxide and dimethoate respectively. Nitrostigmine could not be removed by haemodialysis. These findings suggest that haemodialysis could be of therapeutic value in the treatment of severe demeton-S-methyl sulfoxide and dimethoate poisoning in man. By blood exchange transfusion only 0.06% of the injected dose of nitrostigmine could be removed from the body of poisoned rats. Acetylcholinesterase activity increased only briefly in the period of blood exchange transfusion and decreased gradually afterwards. The enzymatic activity of brain and muscle was unaffected. Therefore, blood exchange transfusion has, if any at all, only poor therapeutic properties in nitrostigmine intoxication.", "contents": "Therapeutic properties.of haemodialysis and blood exchange transfusion in organophosphate poisoning. Human blood was contaminated with nitrostigmine, dimethoate and demeton-S-methyl sulfoxide. It was then dialysed, concentrations of organophosphates were determined and dialysance values calculated. The influence of blood exchange transfusion on poison elimination as well as on the cholinesterase activity of blood, brain and muscle was studied in rats poisoned with nitrostigmine. Haemodialysis was found to be quite an effective method for eliminating demeton-S-methyl sulfoxide and dimethoate, dialysance values of 52.98 ml/min and 59.07 ml/min being found for demeton-S-methyl sulfoxide and dimethoate respectively. Nitrostigmine could not be removed by haemodialysis. These findings suggest that haemodialysis could be of therapeutic value in the treatment of severe demeton-S-methyl sulfoxide and dimethoate poisoning in man. By blood exchange transfusion only 0.06% of the injected dose of nitrostigmine could be removed from the body of poisoned rats. Acetylcholinesterase activity increased only briefly in the period of blood exchange transfusion and decreased gradually afterwards. The enzymatic activity of brain and muscle was unaffected. Therefore, blood exchange transfusion has, if any at all, only poor therapeutic properties in nitrostigmine intoxication.", "PMID": 954759}
{"id": "pubmed23n0032_1316", "title": "Studies in cerebrospinal acid base values in intensive care patients.", "content": "CSF acid base homeostasis was studied in 22 patients in an Intensive Care Unit, to ascertain whether an assessment of this parameter was of any prognostic value. The only significant findings were a lower CSF bicarbonate concentration in those patients who demised, as well as a poor correlation between CSF pH and both CSF PCO2 and bicarbonate. It was concluded that an assessment of CSF acid base balance was of no prognostic value in this particular group of patients.", "contents": "Studies in cerebrospinal acid base values in intensive care patients. CSF acid base homeostasis was studied in 22 patients in an Intensive Care Unit, to ascertain whether an assessment of this parameter was of any prognostic value. The only significant findings were a lower CSF bicarbonate concentration in those patients who demised, as well as a poor correlation between CSF pH and both CSF PCO2 and bicarbonate. It was concluded that an assessment of CSF acid base balance was of no prognostic value in this particular group of patients.", "PMID": 954760}
{"id": "pubmed23n0032_1317", "title": "Continuous intravascular monitoring of pO2 and pCO2. A comparative in vitro-in vivo study.", "content": "Two electrodes placed at the tip of catheters for in vivo determinations of PCO2 and PO2 respectively, were tested in dogs. Results were satisfactory when compared to a highly accurate reference method, correlation coefficients were close to 1 (P less than 10(-9)). Means of the differences were respectively --1.74 +/- 1.14 toor for the PO2 probe (P less than 0.01) and --1.62 +/- 0.72 torr for the PCO2 sensor (P less than 0.0001). While no drift was detected in the PCO2 electrode, that of the PO2 was significant but negligible compared to the variability of measurements. Thus, for PCO2 values between 20 and 85 torr, and PO2 values between 20 and 140 torr, in vivo monitoring is sufficiently reliable for clinical use.", "contents": "Continuous intravascular monitoring of pO2 and pCO2. A comparative in vitro-in vivo study. Two electrodes placed at the tip of catheters for in vivo determinations of PCO2 and PO2 respectively, were tested in dogs. Results were satisfactory when compared to a highly accurate reference method, correlation coefficients were close to 1 (P less than 10(-9)). Means of the differences were respectively --1.74 +/- 1.14 toor for the PO2 probe (P less than 0.01) and --1.62 +/- 0.72 torr for the PCO2 sensor (P less than 0.0001). While no drift was detected in the PCO2 electrode, that of the PO2 was significant but negligible compared to the variability of measurements. Thus, for PCO2 values between 20 and 85 torr, and PO2 values between 20 and 140 torr, in vivo monitoring is sufficiently reliable for clinical use.", "PMID": 954761}
{"id": "pubmed23n0032_1318", "title": "Transcutaneous pO2 monitoring in anaesthesia.", "content": "In 23 patients 18 to 73 years old transcutaneous PO2, relative local perfusion and cardiorespirogram during induction and end stage of anaesthesia were monitored. This method allows continuous sufficiently exact estimation of PaO2. The comparison between tcPO2 and corresponding blood gas analysis from arterial samples showed a good correlation of r=0.94. Thus continuous tcPO2 registration enables quick diagnosis of hypoxia and its therapy.", "contents": "Transcutaneous pO2 monitoring in anaesthesia. In 23 patients 18 to 73 years old transcutaneous PO2, relative local perfusion and cardiorespirogram during induction and end stage of anaesthesia were monitored. This method allows continuous sufficiently exact estimation of PaO2. The comparison between tcPO2 and corresponding blood gas analysis from arterial samples showed a good correlation of r=0.94. Thus continuous tcPO2 registration enables quick diagnosis of hypoxia and its therapy.", "PMID": 954762}
{"id": "pubmed23n0032_1319", "title": "The effect of respiration on the monitoring of stroke volume and cardiac output by the electrical impedance technique.", "content": "In six volunteers (5 male, 1 female) it has been shown that normal respiration made no statistical difference to the estimates of the mean stroke volume and the mean cardiac output as determined by the electrical impedance method of Kubicek et al, (1966). The coefficient of variation was usually increased by respiration. The use of those stroke volumes which occur only at end-expiration was not shown to yield a greater reproducibility with 3 other male volunteers. In the female subject it was found that the use of a digital averager triggered from the preceding R-wave of the ECG gave values for the mean stroke volume and cardiac output which were always lower than the conventional mean values obtained from a number of strokes. The expense of either of these approaches does not appear to be justified as a means of compensating for the effects of normal respiration on the impedance dZ/dt waveform.", "contents": "The effect of respiration on the monitoring of stroke volume and cardiac output by the electrical impedance technique. In six volunteers (5 male, 1 female) it has been shown that normal respiration made no statistical difference to the estimates of the mean stroke volume and the mean cardiac output as determined by the electrical impedance method of Kubicek et al, (1966). The coefficient of variation was usually increased by respiration. The use of those stroke volumes which occur only at end-expiration was not shown to yield a greater reproducibility with 3 other male volunteers. In the female subject it was found that the use of a digital averager triggered from the preceding R-wave of the ECG gave values for the mean stroke volume and cardiac output which were always lower than the conventional mean values obtained from a number of strokes. The expense of either of these approaches does not appear to be justified as a means of compensating for the effects of normal respiration on the impedance dZ/dt waveform.", "PMID": 954763}
{"id": "pubmed23n0032_1320", "title": "Influence of early mobilisation of pulmonary function in surgical patients.", "content": "This work confirms the generally known decrease in arterial oxygen following surgery. On the basis of a more rapid restitution of pulmonary function the superiority of mobilization to the usual respiratory exercises is clearly shown.", "contents": "Influence of early mobilisation of pulmonary function in surgical patients. This work confirms the generally known decrease in arterial oxygen following surgery. On the basis of a more rapid restitution of pulmonary function the superiority of mobilization to the usual respiratory exercises is clearly shown.", "PMID": 954764}
{"id": "pubmed23n0032_1321", "title": "Syndrome of depressed vital functions in the immediate post-operative period of surgical patients: some aspects of its pathogenesis.", "content": "Fifty adult male and female surgical patients (age 25--40 years) undergoing elective and emergency surgical procedures were subjected to isocapnoeic, hypocapnoeic and hypercapnoeic ventilation during anaesthesia; the PaCO2 ranged between 22--90 mm Hg. Three developed the syndrome of depressed vital functions in the post-surgical period by scoring less than 7 out of 10, the others recovered normally with complete resotration of reflex activity, consciousness and skeletomuscular tone, scoring on an average 8 out of 10 points. The role of muscle relaxant drugs in this syndrome and also the depression of the central nervous system is discussed.", "contents": "Syndrome of depressed vital functions in the immediate post-operative period of surgical patients: some aspects of its pathogenesis. Fifty adult male and female surgical patients (age 25--40 years) undergoing elective and emergency surgical procedures were subjected to isocapnoeic, hypocapnoeic and hypercapnoeic ventilation during anaesthesia; the PaCO2 ranged between 22--90 mm Hg. Three developed the syndrome of depressed vital functions in the post-surgical period by scoring less than 7 out of 10, the others recovered normally with complete resotration of reflex activity, consciousness and skeletomuscular tone, scoring on an average 8 out of 10 points. The role of muscle relaxant drugs in this syndrome and also the depression of the central nervous system is discussed.", "PMID": 954765}
{"id": "pubmed23n0032_1322", "title": "Left ventricular function after acute myocardial infarction.", "content": "10 patients with their first AMI were studied within the first 48 hours and again after 3 weeks. Central and peripheral haemodynamics (CI, SV, SW, TPR) were examined, including indices of contractility (dp/dtmax) and wall stiffness (deltaP/deltaV, relation deltaP/deltaV to P) of the left ventricle. In the early phase CI and SW, as well as LV dp/dtmax were depressed in accordance with symptoms of LV failure. deltaP/deltaV was increased. Elevation of LVEDP correlated well with ventricular gallop rhythm, but less consistently with LV functional disturbance. During convalescence CI increased uniformly, both in digitalized and non-digitalized individuals. In contrast heart rate, aortic pressure, LVEDP and dp/dtmax remained unchanged. The increase of CI, SV and SW was accompanied by a fall of TPR and deltaP/deltaV. LV wall stiffness was still  elevated above normal after 3 weeks. The improvement of cardiac pumping during infarct convalescence may have been effected through a fall of TPR and LV wall stiffness. Recovery of depressed contractile performance was generally not observed, and does therefore not seem to contribute to recuperation.", "contents": "Left ventricular function after acute myocardial infarction. 10 patients with their first AMI were studied within the first 48 hours and again after 3 weeks. Central and peripheral haemodynamics (CI, SV, SW, TPR) were examined, including indices of contractility (dp/dtmax) and wall stiffness (deltaP/deltaV, relation deltaP/deltaV to P) of the left ventricle. In the early phase CI and SW, as well as LV dp/dtmax were depressed in accordance with symptoms of LV failure. deltaP/deltaV was increased. Elevation of LVEDP correlated well with ventricular gallop rhythm, but less consistently with LV functional disturbance. During convalescence CI increased uniformly, both in digitalized and non-digitalized individuals. In contrast heart rate, aortic pressure, LVEDP and dp/dtmax remained unchanged. The increase of CI, SV and SW was accompanied by a fall of TPR and deltaP/deltaV. LV wall stiffness was still  elevated above normal after 3 weeks. The improvement of cardiac pumping during infarct convalescence may have been effected through a fall of TPR and LV wall stiffness. Recovery of depressed contractile performance was generally not observed, and does therefore not seem to contribute to recuperation.", "PMID": 954767}
{"id": "pubmed23n0032_1323", "title": "Studies of malformation syndromes of man XXXIX: a craniosynostosis-craniofacial dysostosis syndrome with mental retardation and other malformations: \"craniofacial dyssynostosis\".", "content": "We report clinical findings in 2 sisters and 5 sporadic cases with a \"new\" type of craniosynostosis/craniofacial dysostosis and shortness of stature. Premature closure of lambdoid sutures and posterior part of sagittal suture causes a posteriorly narrow, dolichocephalic skull with small, flat or bulging occiput and protuberance of the forehead; disturbance of the growth of basal skull structures leads to craniofacial dysostosis and (secondary) anomalies of the face. In one patient the coronal suture was also involved. One of the patients had a congenital heart defect. Four untreated patients had mental retardation; 3 had craniosynostectomy with more or less normal psychomotor development afterwards. Some patients had hydrocephalus and 1 had a brain malformation (agenesis of the corpus callosum with presumed interventricular lipoma). The observation of sisters with the same condition suggests autosomal recessive inheritance. This etiologic hypothesis is supported by the fact that 4 of 7 patients are of Spanish, Mexican, or Puerto Rican ancestry; this population probably has a rather high gene frequency and the trait should be relatively common in areas occupied by this population and their descendents. The condition has been designated craniofacial dyssynostosis.", "contents": "Studies of malformation syndromes of man XXXIX: a craniosynostosis-craniofacial dysostosis syndrome with mental retardation and other malformations: \"craniofacial dyssynostosis\". We report clinical findings in 2 sisters and 5 sporadic cases with a \"new\" type of craniosynostosis/craniofacial dysostosis and shortness of stature. Premature closure of lambdoid sutures and posterior part of sagittal suture causes a posteriorly narrow, dolichocephalic skull with small, flat or bulging occiput and protuberance of the forehead; disturbance of the growth of basal skull structures leads to craniofacial dysostosis and (secondary) anomalies of the face. In one patient the coronal suture was also involved. One of the patients had a congenital heart defect. Four untreated patients had mental retardation; 3 had craniosynostectomy with more or less normal psychomotor development afterwards. Some patients had hydrocephalus and 1 had a brain malformation (agenesis of the corpus callosum with presumed interventricular lipoma). The observation of sisters with the same condition suggests autosomal recessive inheritance. This etiologic hypothesis is supported by the fact that 4 of 7 patients are of Spanish, Mexican, or Puerto Rican ancestry; this population probably has a rather high gene frequency and the trait should be relatively common in areas occupied by this population and their descendents. The condition has been designated craniofacial dyssynostosis.", "PMID": 954768}
{"id": "pubmed23n0032_1324", "title": "Chondrodysplastic dwarfism, cleft palate and micrognathia in a neonate, a new syndrome?", "content": "We describe a female infant with lethal, short-limb dwarfism, micrognathia, hydrocephalus with occipital encephalocele and a generalized spondyloepimetaphyseal dysplasia who probably has the same condition that was described by Rolland et al. (1972) and by Langer et al. (1976). This may be recessively inherited syndrome", "contents": "Chondrodysplastic dwarfism, cleft palate and micrognathia in a neonate, a new syndrome? We describe a female infant with lethal, short-limb dwarfism, micrognathia, hydrocephalus with occipital encephalocele and a generalized spondyloepimetaphyseal dysplasia who probably has the same condition that was described by Rolland et al. (1972) and by Langer et al. (1976). This may be recessively inherited syndrome", "PMID": 954769}
{"id": "pubmed23n0032_1325", "title": "Severe anemia due to transient pure red cell aplasia in early childhood. Arrest at the level of the committed stem cells?", "content": "Five patients, 11 months to 3 4/12 years old with severe normochromic, normocytis anemia and reticulocytopenia are reported. At the height of erythropoietic arrest when erythroid precursor cells were completely absent, undifferentiated stem cells (transitional cells), accumulated in the bone marrow. They disappeared again upon spontaneous resumption of erythropoiesis. We suggest that the erythropoietic arrest had occurred at the level of the hematopoietic stem cell. All patients recovered within 1 week of diagnosis. No steroid therapy was given. Failure to recognize this clinical entity leads to unnecessary diagnostic and therapeutic procedures including the transfusion of blood.", "contents": "Severe anemia due to transient pure red cell aplasia in early childhood. Arrest at the level of the committed stem cells? Five patients, 11 months to 3 4/12 years old with severe normochromic, normocytis anemia and reticulocytopenia are reported. At the height of erythropoietic arrest when erythroid precursor cells were completely absent, undifferentiated stem cells (transitional cells), accumulated in the bone marrow. They disappeared again upon spontaneous resumption of erythropoiesis. We suggest that the erythropoietic arrest had occurred at the level of the hematopoietic stem cell. All patients recovered within 1 week of diagnosis. No steroid therapy was given. Failure to recognize this clinical entity leads to unnecessary diagnostic and therapeutic procedures including the transfusion of blood.", "PMID": 954770}
{"id": "pubmed23n0032_1326", "title": "The 18 p-syndrome. Report of four cases.", "content": "Four children, two girls and two boys, were found to have a short arm deletion of chromosome No. 18. Three of them exhibit a typical dysmorphy of the face showing retraction of the midface, broad-based, flat nose, hypertelorism, epicanthus, \"carp mouth\", big, protruding, and low set ears, as well as a variable number of Turner-like features, failure of growth, mental retardation, and muscular hypotonia. A newly born child, who died at 2 days of age exhibited severe brain defects of holoprosencephalic series. The clinical and cytogenetic findings are compared with the reviewed data of the 18 p deletion. The hypothesis of \"gene-dosis compensation\" is discussed in order to explain the variable phenotypical expression of 18 p-syndrome as there is obviously to correlation between the extent of the deficiency and the expression of malformations.", "contents": "The 18 p-syndrome. Report of four cases. Four children, two girls and two boys, were found to have a short arm deletion of chromosome No. 18. Three of them exhibit a typical dysmorphy of the face showing retraction of the midface, broad-based, flat nose, hypertelorism, epicanthus, \"carp mouth\", big, protruding, and low set ears, as well as a variable number of Turner-like features, failure of growth, mental retardation, and muscular hypotonia. A newly born child, who died at 2 days of age exhibited severe brain defects of holoprosencephalic series. The clinical and cytogenetic findings are compared with the reviewed data of the 18 p deletion. The hypothesis of \"gene-dosis compensation\" is discussed in order to explain the variable phenotypical expression of 18 p-syndrome as there is obviously to correlation between the extent of the deficiency and the expression of malformations.", "PMID": 954771}
{"id": "pubmed23n0032_1327", "title": "Hereditary Amyotrophic Lateral Sclerosis. A report of two families.", "content": "An aggregation of 14 cases of amyotrophic lateral sclerosis (ALS) was encountered in two families in Minnesota. Although the classical clinical features of ALS predominated, some members of one family showed, in addition, extrapyramidal signs, peripheral sensory impairment in the upper and lower limbs and mild mental fallout. Autosomal dominant inheritance with incomplete penetrance was the most likely mode of transmission. Pathological changes were the same as those seen in sporadic ALS although one patient also showed degeneration of the substantia nigra. These two families were compared to others in the literature and an effort was made to refine the classification of familial ALS.", "contents": "Hereditary Amyotrophic Lateral Sclerosis. A report of two families. An aggregation of 14 cases of amyotrophic lateral sclerosis (ALS) was encountered in two families in Minnesota. Although the classical clinical features of ALS predominated, some members of one family showed, in addition, extrapyramidal signs, peripheral sensory impairment in the upper and lower limbs and mild mental fallout. Autosomal dominant inheritance with incomplete penetrance was the most likely mode of transmission. Pathological changes were the same as those seen in sporadic ALS although one patient also showed degeneration of the substantia nigra. These two families were compared to others in the literature and an effort was made to refine the classification of familial ALS.", "PMID": 954772}
{"id": "pubmed23n0032_1328", "title": "Multiple sclerosis, diffuse lymphoplasmocytic encephalitis and Alzheimer's disease in a patient with progressive dementia.", "content": "Female patient, aged 63 died after 5 years of progressive dementia. Autopsy revealed multiple sclerosis, diffuse lymphoplasmocytic encephalitis, and Alzheimer's disease. Possible pathogenetic connections between these disease entities are discussed.", "contents": "Multiple sclerosis, diffuse lymphoplasmocytic encephalitis and Alzheimer's disease in a patient with progressive dementia. Female patient, aged 63 died after 5 years of progressive dementia. Autopsy revealed multiple sclerosis, diffuse lymphoplasmocytic encephalitis, and Alzheimer's disease. Possible pathogenetic connections between these disease entities are discussed.", "PMID": 954773}
{"id": "pubmed23n0032_1329", "title": "Myasthenic syndrome in polymyositis.", "content": "The case report of a 23-year-old man with polymyositis and some myasthenic features is presented. The biopsy findings suggest a preferential involvement of the neuromuscular junctions by the inflammatory process, a fact which explains the clinical syndrome.", "contents": "Myasthenic syndrome in polymyositis. The case report of a 23-year-old man with polymyositis and some myasthenic features is presented. The biopsy findings suggest a preferential involvement of the neuromuscular junctions by the inflammatory process, a fact which explains the clinical syndrome.", "PMID": 954774}
{"id": "pubmed23n0032_1330", "title": "Congenital non-progressive myopathy with type II fibre atrophy and internal nuclei.", "content": "A particular form of congenital non-progressive myopathy in a 5-year-old boy is described. The morphological features include predominant atrophy and rarity of type II muscle fibres, internally placed nuclei and myofibrillar degeneration in affected fibres.", "contents": "Congenital non-progressive myopathy with type II fibre atrophy and internal nuclei. A particular form of congenital non-progressive myopathy in a 5-year-old boy is described. The morphological features include predominant atrophy and rarity of type II muscle fibres, internally placed nuclei and myofibrillar degeneration in affected fibres.", "PMID": 954775}
{"id": "pubmed23n0032_1331", "title": "A solitary abscess of the medulla oblongata.", "content": "A neuropathological description is presented of a solitary giant abscess located in the oblongate medulla of a child aged two. This abscess destroyed almost all the morphological structures in the medulla and caused considerable constriction and translocation of the IVth ventricle. Apart from the exceptional occurrence of an abscess in this brain region, the interesting features of this case are the slow development of the abscess (probably 8 months) and the existence of a well-formed connective tissue capsule, demarcating the lesion from its surroundings. The diagnostic difficulties and clinical and histopathological correlation are also discussed.", "contents": "A solitary abscess of the medulla oblongata. A neuropathological description is presented of a solitary giant abscess located in the oblongate medulla of a child aged two. This abscess destroyed almost all the morphological structures in the medulla and caused considerable constriction and translocation of the IVth ventricle. Apart from the exceptional occurrence of an abscess in this brain region, the interesting features of this case are the slow development of the abscess (probably 8 months) and the existence of a well-formed connective tissue capsule, demarcating the lesion from its surroundings. The diagnostic difficulties and clinical and histopathological correlation are also discussed.", "PMID": 954776}
{"id": "pubmed23n0032_1332", "title": "Material and design factors in thromboembolization in total artificial heart recipients living 100-2,000 hours.", "content": "When total artificial heart (TAH) recipients live over 100 h it is possible to evaluate the thrombogenicity of design and biomaterials. In 20 consecutive TAH recipients of the Jarvik heart with Bj\u00f6rk-Shiley valves we compared rough (Dacron fibril-coated Silastic) and smooth (Biomer and Avcothane) blood surfaces. The mean survival times with rough surfaces was 296 h, with smooth surfaces 545 h. The present designs necessitate a sharp angle junction between the diaphragm and housing, creating stagnation areas and turbulences which induce thromboembolization. The valve rings and struts are another constant source of thromboembolism. Of the 80 valves, 41% showed thrombus deposition independent of anticoagulation. The polyurethane surfaces, in contrast to the fibrilized surfaces, were very clean and free of thrombi at autopsy. The thromboembolization was not dependent on anticoagulation and platelet adhesiveness inhibitor in hearts with rough surfaces. Based on 20 experiments including 78- and 94-day TAH survivors (Avcothane and Biomer hearts), there is an indication of the need of a new ventricular design. The ultimate design should generate physiological pulse waves without turbulences or stagnation areas within the device.", "contents": "Material and design factors in thromboembolization in total artificial heart recipients living 100-2,000 hours. When total artificial heart (TAH) recipients live over 100 h it is possible to evaluate the thrombogenicity of design and biomaterials. In 20 consecutive TAH recipients of the Jarvik heart with Bj\u00f6rk-Shiley valves we compared rough (Dacron fibril-coated Silastic) and smooth (Biomer and Avcothane) blood surfaces. The mean survival times with rough surfaces was 296 h, with smooth surfaces 545 h. The present designs necessitate a sharp angle junction between the diaphragm and housing, creating stagnation areas and turbulences which induce thromboembolization. The valve rings and struts are another constant source of thromboembolism. Of the 80 valves, 41% showed thrombus deposition independent of anticoagulation. The polyurethane surfaces, in contrast to the fibrilized surfaces, were very clean and free of thrombi at autopsy. The thromboembolization was not dependent on anticoagulation and platelet adhesiveness inhibitor in hearts with rough surfaces. Based on 20 experiments including 78- and 94-day TAH survivors (Avcothane and Biomer hearts), there is an indication of the need of a new ventricular design. The ultimate design should generate physiological pulse waves without turbulences or stagnation areas within the device.", "PMID": 954777}
{"id": "pubmed23n0032_1333", "title": "Comparative study of in vitro flow characteristics between a human aortic valve and a designed aortic valve and six corresponding types of prosthetic heart valves.", "content": "A model of a pulsatile cardiovascular system is described including an electronic control, a hydraulic driving unit, a volume storage, a peripheral resistance and measuring equipment. Six different artificial heart valves, a designed aortic valve (Aachen pockett valve II) and a human aortic valve have been tested in the outflow position of the pump. Three of them are presently being used in clinical applications. Measurements of pressure and volume flow have been obtained for different frequencies n, pump volumes Vp and time ratios between pressure and suction phase. From the experimental results a comparative evaluation was deducted for different working conditions. In comparison to natural human aortic valve (NK) large volume flow is verified by the Aachen pocket valve II (AT), the Gott-Dagget value (GD), the Starr-Edwards valve (SE) and the Lillehei-Kaster valve (LK). The Gott-Daggett valve has the smallest back flow volume. The Smeloff-Cutter and in particular the Wada-Cutter valve have the largest regurgitation. This is to be expected since the Cutter valves are purposely so designed that they do not close tightly in order to avoid blood sedimentation. The human aortic valve, the Gott-Daggett valve and the Aachen pocket valve demonstrate the most favourable opening-closing characteristics. The human aortic valve achieves a negligible pressure loss, followed by the Bj\u00f6rk-Shiley and the Lillehei-Kaster valve.", "contents": "Comparative study of in vitro flow characteristics between a human aortic valve and a designed aortic valve and six corresponding types of prosthetic heart valves. A model of a pulsatile cardiovascular system is described including an electronic control, a hydraulic driving unit, a volume storage, a peripheral resistance and measuring equipment. Six different artificial heart valves, a designed aortic valve (Aachen pockett valve II) and a human aortic valve have been tested in the outflow position of the pump. Three of them are presently being used in clinical applications. Measurements of pressure and volume flow have been obtained for different frequencies n, pump volumes Vp and time ratios between pressure and suction phase. From the experimental results a comparative evaluation was deducted for different working conditions. In comparison to natural human aortic valve (NK) large volume flow is verified by the Aachen pocket valve II (AT), the Gott-Dagget value (GD), the Starr-Edwards valve (SE) and the Lillehei-Kaster valve (LK). The Gott-Daggett valve has the smallest back flow volume. The Smeloff-Cutter and in particular the Wada-Cutter valve have the largest regurgitation. This is to be expected since the Cutter valves are purposely so designed that they do not close tightly in order to avoid blood sedimentation. The human aortic valve, the Gott-Daggett valve and the Aachen pocket valve demonstrate the most favourable opening-closing characteristics. The human aortic valve achieves a negligible pressure loss, followed by the Bj\u00f6rk-Shiley and the Lillehei-Kaster valve.", "PMID": 954778}
{"id": "pubmed23n0032_1334", "title": "Effect of low molecular dextran on total liver blood flow in hemorrhagic shock.", "content": "Hemorrhage amounting to 40% of the calculated blood volume in dogs produced a fall in systemic blood pressure to a level of approximately 50 mm Hg. This was associated with a decline of total liver blood flow of about 60% of normal. Infusion of low molecular dextran (LMD) or saline of a volume, corresponding to 1/7 of the volume of blood taken out, increased the total liver blood flow. This increase was much more pronounced in the LMD group, maybe due to the ability of LMD to simultaneously increase systemic blood pressure, decrease blood viscosity and disaggregate red blood cells in the microcirculation.", "contents": "Effect of low molecular dextran on total liver blood flow in hemorrhagic shock. Hemorrhage amounting to 40% of the calculated blood volume in dogs produced a fall in systemic blood pressure to a level of approximately 50 mm Hg. This was associated with a decline of total liver blood flow of about 60% of normal. Infusion of low molecular dextran (LMD) or saline of a volume, corresponding to 1/7 of the volume of blood taken out, increased the total liver blood flow. This increase was much more pronounced in the LMD group, maybe due to the ability of LMD to simultaneously increase systemic blood pressure, decrease blood viscosity and disaggregate red blood cells in the microcirculation.", "PMID": 954779}
{"id": "pubmed23n0032_1335", "title": "An attempt to induce portal hypertension and esophageal varices in the rat.", "content": "An attempt to induce portal hypertension using Ameroid constrictors and shunt operations is described. 1 week after the application of the Ameroid constrictor around the portal trunk, it was completely occluded and portal venous pressure was elevated to a level about twice of normal range. Angiographically, splenorenal collaterals and collaterals overbridging the Ameroid constrictor were observed in all examined rats. However, esophageal varices could not be found in any rat. In the second series of the experiment, an end-to-side cavoportal shunt was created before the application of the Ameroid constrictor. This operation model was devised to increase the portal venous flow. At the same time this operation should inhibit the development of splenorenal collaterals and as expected collaterals to the superior vena cava system developed instead. However, they were not located in the esophageal wall but retroperitoneally.", "contents": "An attempt to induce portal hypertension and esophageal varices in the rat. An attempt to induce portal hypertension using Ameroid constrictors and shunt operations is described. 1 week after the application of the Ameroid constrictor around the portal trunk, it was completely occluded and portal venous pressure was elevated to a level about twice of normal range. Angiographically, splenorenal collaterals and collaterals overbridging the Ameroid constrictor were observed in all examined rats. However, esophageal varices could not be found in any rat. In the second series of the experiment, an end-to-side cavoportal shunt was created before the application of the Ameroid constrictor. This operation model was devised to increase the portal venous flow. At the same time this operation should inhibit the development of splenorenal collaterals and as expected collaterals to the superior vena cava system developed instead. However, they were not located in the esophageal wall but retroperitoneally.", "PMID": 954780}
{"id": "pubmed23n0032_1336", "title": "Exercise fitness, cardiac work and blood viscosity factors in patients and normals.", "content": "A study of 41 cardiovascular patients and normals showed significant correlations between physical fitness (as expressed by a fitness index based on work output) and high shear rate blood viscosity (p less than 0.01); between the cardiac work expressed as double product (= myocardial oxygen demand) and aggregation of red cells (p less than 0.05); between the triple term of cardiac work (fitness index divided by double product) and high shear rate blood viscosity (p less than 0.005) or aggregation of red cells (p less than 0.05), in all cases correlations being negative i.e., higher cardiac work or higher fitness being related to low blood viscosity or lower aggregation of red cells. Significant differences were found between high-fitness and low-fitness groups, the high-fitness group showing from plasma viscosity (p less than 0.0005), lower fibrinogen level (p less than 0.05), and higher albumin/fibrinogen ratio (p less than 0.01).", "contents": "Exercise fitness, cardiac work and blood viscosity factors in patients and normals. A study of 41 cardiovascular patients and normals showed significant correlations between physical fitness (as expressed by a fitness index based on work output) and high shear rate blood viscosity (p less than 0.01); between the cardiac work expressed as double product (= myocardial oxygen demand) and aggregation of red cells (p less than 0.05); between the triple term of cardiac work (fitness index divided by double product) and high shear rate blood viscosity (p less than 0.005) or aggregation of red cells (p less than 0.05), in all cases correlations being negative i.e., higher cardiac work or higher fitness being related to low blood viscosity or lower aggregation of red cells. Significant differences were found between high-fitness and low-fitness groups, the high-fitness group showing from plasma viscosity (p less than 0.0005), lower fibrinogen level (p less than 0.05), and higher albumin/fibrinogen ratio (p less than 0.01).", "PMID": 954781}
{"id": "pubmed23n0032_1337", "title": "The place of gastrin determination in ulcer surgery.", "content": "This paper is concerned with the place of gastrin determination in ulcer surgery. Only the acid-stimulating properties of gastrin are considered. The role of the vagus in the gastrin response to a test meal was investigated in patients with duodenal ulcer. 50 patients were studied before and 3, 6, and 12 months after highly selective vagotomy without (25 patients) and with Wnagensteen-pyloroplasty (25 patients). Basal and stimulated gastrin concentration in serum, as determined by radioimmunoassay, were higher 3 months after the operation and 3h after the test meal gastrin levels were still 50% higher than the basal levels. No difference was found between the patients treated with highly selective vagotomy and the patients treates with HSV and additional pyloroplasty. 6 and 12 months after the operation a moderate decrease of gastrin secretion was observed, but basal as well as stimulated levels were still elevated after 12 months compared to the values before operation. Our results indicate that the pattern of gastrin secretion changes as time passes after highly selective vagotomy with or without additional pyloroplasty.", "contents": "The place of gastrin determination in ulcer surgery. This paper is concerned with the place of gastrin determination in ulcer surgery. Only the acid-stimulating properties of gastrin are considered. The role of the vagus in the gastrin response to a test meal was investigated in patients with duodenal ulcer. 50 patients were studied before and 3, 6, and 12 months after highly selective vagotomy without (25 patients) and with Wnagensteen-pyloroplasty (25 patients). Basal and stimulated gastrin concentration in serum, as determined by radioimmunoassay, were higher 3 months after the operation and 3h after the test meal gastrin levels were still 50% higher than the basal levels. No difference was found between the patients treated with highly selective vagotomy and the patients treates with HSV and additional pyloroplasty. 6 and 12 months after the operation a moderate decrease of gastrin secretion was observed, but basal as well as stimulated levels were still elevated after 12 months compared to the values before operation. Our results indicate that the pattern of gastrin secretion changes as time passes after highly selective vagotomy with or without additional pyloroplasty.", "PMID": 954783}
{"id": "pubmed23n0032_1338", "title": "Modification of procine stress ulceration by methylprednisolone, vitaminA and methysergide treatment.", "content": "Using a swine shock ulcer model, three pharmacological agents, methylprednisolone, vitamin A and methysergide were evaluated, as they protect gastric mucosa against acute ulceration. Following haemorrhagic shock (3 h duration; mean arterial pressure 40 mm Hg) nine of the ten control animals (90%) developed gastric ulceration. Of the six test animals treated with intravenous methylprednisolone during the shock, only one (17%) developed gastric lesions (p less than 0.02; x2 = 5.76). Of the ten test animals pretreated with massive doses of parenteral vitamin A, only three (30%) developed lesions (p less than 0.05; x2 = 5.21). In contrast to this, treatment with methysergide, a serotonin antagonist, did not significantly effect the ulceration rate, since four of the six test animals (67%) had gastric lesions. The results suggest that methylprednisolone and vitamin A do protect the gastric mucosa from experiment stress ulceration, but their mechanism of action remain obscure and further investigation is needed to judge their value in clinical use.", "contents": "Modification of procine stress ulceration by methylprednisolone, vitaminA and methysergide treatment. Using a swine shock ulcer model, three pharmacological agents, methylprednisolone, vitamin A and methysergide were evaluated, as they protect gastric mucosa against acute ulceration. Following haemorrhagic shock (3 h duration; mean arterial pressure 40 mm Hg) nine of the ten control animals (90%) developed gastric ulceration. Of the six test animals treated with intravenous methylprednisolone during the shock, only one (17%) developed gastric lesions (p less than 0.02; x2 = 5.76). Of the ten test animals pretreated with massive doses of parenteral vitamin A, only three (30%) developed lesions (p less than 0.05; x2 = 5.21). In contrast to this, treatment with methysergide, a serotonin antagonist, did not significantly effect the ulceration rate, since four of the six test animals (67%) had gastric lesions. The results suggest that methylprednisolone and vitamin A do protect the gastric mucosa from experiment stress ulceration, but their mechanism of action remain obscure and further investigation is needed to judge their value in clinical use.", "PMID": 954784}
{"id": "pubmed23n0032_1339", "title": "Insulin response and portal-peripheral insulin difference during the oral glucose tolerance test in patients after abdominal operations.", "content": "Following abdominal surgery, insulin and glucose concentrations in the portal vein, and a peripheral vein are compared in patients during control periods and after oral administration of glucose. During the control period, the glucose concentrations are identical in both veins. After glucose loads with the prompt increase of portal glucose concentration the portal-peripheral difference also increases (p less than 0.01). During the control period the insulin concentration in the portal vein is double as compared to peripheral blood (p less than 0.005). After glucose load the increasing portal insulin as well as the peripheral and portal glucose correlate with the portal-peripheral insulin difference (p less than 0.001). Furthermore, there is a significant positive correlation between the peripheral glucose area as a parameter of glucose tolerance and the portal insulin area as a semiquantitative parameter of insulin secretory capacity (p less than 0.001). It can be concluded that in the early postoperative period in patients with a diminished oral glucose tolerance (large glucose areas) there is an even greater insulin response in comparison to patients with normal oral glucose tolerance. On the other hand, however, in those patients with diminished glucose tolerance, the insulin response is essentially delayed.", "contents": "Insulin response and portal-peripheral insulin difference during the oral glucose tolerance test in patients after abdominal operations. Following abdominal surgery, insulin and glucose concentrations in the portal vein, and a peripheral vein are compared in patients during control periods and after oral administration of glucose. During the control period, the glucose concentrations are identical in both veins. After glucose loads with the prompt increase of portal glucose concentration the portal-peripheral difference also increases (p less than 0.01). During the control period the insulin concentration in the portal vein is double as compared to peripheral blood (p less than 0.005). After glucose load the increasing portal insulin as well as the peripheral and portal glucose correlate with the portal-peripheral insulin difference (p less than 0.001). Furthermore, there is a significant positive correlation between the peripheral glucose area as a parameter of glucose tolerance and the portal insulin area as a semiquantitative parameter of insulin secretory capacity (p less than 0.001). It can be concluded that in the early postoperative period in patients with a diminished oral glucose tolerance (large glucose areas) there is an even greater insulin response in comparison to patients with normal oral glucose tolerance. On the other hand, however, in those patients with diminished glucose tolerance, the insulin response is essentially delayed.", "PMID": 954785}
{"id": "pubmed23n0032_1340", "title": "Metabolism in the hypothermically perfused kidney. Production and utilization of lactate and utilization of acetate in the dog kidney.", "content": "The mechanism for the high lactate production during hypothermic kidney perfusion has not been clarified previously. The metabolism of lactate and acetate was studied in 23 dog kidneys during continuous hypothermic perfusion. The perfusions were performed in a Gambro machine with a perfusate based on human serum albumin. With a perfusate containing fatty acid extracted albumin, which was almost free of fatty acids, the glucose uptake of the kidney was more pronounced than during perfusion with a fatty acid-rich perfusate. The high glucose uptake under this perfusion condition was associated with a lower lactate production and a higher glucose oxidation rate. In perfusions with a perfusate containing lactate at a concentration of 2.5 mmol/l a considerable lactate uptake of the kidney was shown. By isotope dilution technique the production and uptake rate of lactate was estimated at 4.4 and 8.0 mumol/g kidney and day in two experiments. The labeled lactate carbon was recovered in CO2, and glucose in the perfusate indicating a continuous oxidation and gluconeogenesis. Acetate was used by the kidney both for oxidation and for gluconeogenesis. Addition of acetate to the ordinary fatty acid-rich perfusate caused an enhanced lactate production from the perfused kidney. The results indicate that the high lactate production during hypothermic perfusion of kidneys is mainly dependent on a metabolic blockade at the level of pyruvate dehydrogenase.", "contents": "Metabolism in the hypothermically perfused kidney. Production and utilization of lactate and utilization of acetate in the dog kidney. The mechanism for the high lactate production during hypothermic kidney perfusion has not been clarified previously. The metabolism of lactate and acetate was studied in 23 dog kidneys during continuous hypothermic perfusion. The perfusions were performed in a Gambro machine with a perfusate based on human serum albumin. With a perfusate containing fatty acid extracted albumin, which was almost free of fatty acids, the glucose uptake of the kidney was more pronounced than during perfusion with a fatty acid-rich perfusate. The high glucose uptake under this perfusion condition was associated with a lower lactate production and a higher glucose oxidation rate. In perfusions with a perfusate containing lactate at a concentration of 2.5 mmol/l a considerable lactate uptake of the kidney was shown. By isotope dilution technique the production and uptake rate of lactate was estimated at 4.4 and 8.0 mumol/g kidney and day in two experiments. The labeled lactate carbon was recovered in CO2, and glucose in the perfusate indicating a continuous oxidation and gluconeogenesis. Acetate was used by the kidney both for oxidation and for gluconeogenesis. Addition of acetate to the ordinary fatty acid-rich perfusate caused an enhanced lactate production from the perfused kidney. The results indicate that the high lactate production during hypothermic perfusion of kidneys is mainly dependent on a metabolic blockade at the level of pyruvate dehydrogenase.", "PMID": 954786}
{"id": "pubmed23n0032_1341", "title": "Skeletal muscle metabolites as possible indicators of imminent death in acute hemorrhage.", "content": "The extent to which changes in tissue energy metabolism correspond to the severity of a hemorrhagic shock condition has been studied. In cats, 50% of the blood volume was withdrawn within 10 min, resulting in a fatal outcome within 3h (range from 45 min to 3 h). Skeletal muscle and blood samples were taken prior to the hemorrhagic and in the agonal phase or after 2 h in compensated bled animals. Tissue levels of ATP, CrP, G-6-P, lactate and glucose as well as blood levels of glucose, lactate and pyruvate were determined enzymatically. The results showed no depletion of phosphagen levels in the agonal phase, while glycolytic metabolites, lactate in particular, demonstrated a close correlation to circulatory deterioration and imminent death.", "contents": "Skeletal muscle metabolites as possible indicators of imminent death in acute hemorrhage. The extent to which changes in tissue energy metabolism correspond to the severity of a hemorrhagic shock condition has been studied. In cats, 50% of the blood volume was withdrawn within 10 min, resulting in a fatal outcome within 3h (range from 45 min to 3 h). Skeletal muscle and blood samples were taken prior to the hemorrhagic and in the agonal phase or after 2 h in compensated bled animals. Tissue levels of ATP, CrP, G-6-P, lactate and glucose as well as blood levels of glucose, lactate and pyruvate were determined enzymatically. The results showed no depletion of phosphagen levels in the agonal phase, while glycolytic metabolites, lactate in particular, demonstrated a close correlation to circulatory deterioration and imminent death.", "PMID": 954787}
{"id": "pubmed23n0032_1342", "title": "Wound healing. Comparison of healing rates of burn wounds and of excisional wounds.", "content": "The process of healing of skin excisions and skin burns of equal sizes has been compared in rabbits. Excisions heal definitely quicker than burns, despite the fact that the part of the skin exposed to the burning agent does not undergo necrosis. Small excisional wounds heal more rapidly than larger ones but the healing rate of burn wounds in our series was independent of their size. Secondary excision of the burned tissue accelerates the healing process. Thus the healing time of burn wounds seems to depend more on clearing the wounds of the necrotic tissue than on the mere repair of the skin loss.", "contents": "Wound healing. Comparison of healing rates of burn wounds and of excisional wounds. The process of healing of skin excisions and skin burns of equal sizes has been compared in rabbits. Excisions heal definitely quicker than burns, despite the fact that the part of the skin exposed to the burning agent does not undergo necrosis. Small excisional wounds heal more rapidly than larger ones but the healing rate of burn wounds in our series was independent of their size. Secondary excision of the burned tissue accelerates the healing process. Thus the healing time of burn wounds seems to depend more on clearing the wounds of the necrotic tissue than on the mere repair of the skin loss.", "PMID": 954788}
{"id": "pubmed23n0032_1343", "title": "Motility depressed intestinal propulsion following selective jejunal myotomy in the rat.", "content": "The effect of segmentary sectioning of the proximal jejunal tunica muscularis on intestinal propulsion was studied in the rat. Care was taken to avoid damage to the mucosa. In no instance was perforation or occlusion of the intestine observed. Myotomy was followed by a significant slackening of the passage of a test meal during the first 15 min after feeding, while the total transit time was virtually unchanged. Sectioning the musculature of the intestinal wall is shown to be responsible for retardation of bowel passage.", "contents": "Motility depressed intestinal propulsion following selective jejunal myotomy in the rat. The effect of segmentary sectioning of the proximal jejunal tunica muscularis on intestinal propulsion was studied in the rat. Care was taken to avoid damage to the mucosa. In no instance was perforation or occlusion of the intestine observed. Myotomy was followed by a significant slackening of the passage of a test meal during the first 15 min after feeding, while the total transit time was virtually unchanged. Sectioning the musculature of the intestinal wall is shown to be responsible for retardation of bowel passage.", "PMID": 954789}
{"id": "pubmed23n0032_1344", "title": "Bone mass in maintenance haemodialysis. Prospective study with sequential biopsies.", "content": "The change of bone mass during maintenance haemodialysis was studied in 20 patients. Iliac crest biopsies were obtained at a 9 - 16 months interval. Two biopsies were taken in order to give an independent estimate of the sampling error. 22 individuals in the same age group without skeletal disease who died from traffic accidents or from violent deaths were used as controls. Undecalcified sections of bone (Masson-Goldner-stain) were evaluated by micromorphometry. At the beginning of the study osteosclerosis (i.e. volumetric density of bone above x +/- 2 SD in control patients) was found in 14 out of 20 patients. Volumetric density of bone only increased in one of the patients during the observation period. A significant decrease of volumetric density of bone was found in 5 out of 20 patients. This could be attributed to prolonged immobilisation in two cases and to inadequate protein- and calorie-intake in two cases. No predisposing factor could be found in the fifth case. It is concluded that loss of spongy bone mass (osteopenia) is not an invariable result of haemodialysis.", "contents": "Bone mass in maintenance haemodialysis. Prospective study with sequential biopsies. The change of bone mass during maintenance haemodialysis was studied in 20 patients. Iliac crest biopsies were obtained at a 9 - 16 months interval. Two biopsies were taken in order to give an independent estimate of the sampling error. 22 individuals in the same age group without skeletal disease who died from traffic accidents or from violent deaths were used as controls. Undecalcified sections of bone (Masson-Goldner-stain) were evaluated by micromorphometry. At the beginning of the study osteosclerosis (i.e. volumetric density of bone above x +/- 2 SD in control patients) was found in 14 out of 20 patients. Volumetric density of bone only increased in one of the patients during the observation period. A significant decrease of volumetric density of bone was found in 5 out of 20 patients. This could be attributed to prolonged immobilisation in two cases and to inadequate protein- and calorie-intake in two cases. No predisposing factor could be found in the fifth case. It is concluded that loss of spongy bone mass (osteopenia) is not an invariable result of haemodialysis.", "PMID": 954802}
{"id": "pubmed23n0032_1345", "title": "Activity of erythropoietin and renin in renal venous blood of hypertensive patients.", "content": "Plasma activities of renin and erythropoietin were determined in the renal veins of the right and left kidneys of hypertensive patients. The patients were divided into the following groups either according to the origin of the hypertension (group 1: control for essential hypertension, group 2: renovascular hypertension) or according to the hormone levels (group 3: renin activity exceeding 10 ng/litre in at least one of the renal veins and group 4: erythropoietin activity higher than 4% 59Fe incorporation in at least one of the renal veins). In groups 1, 2 and 3 a statistically significant difference in renin activity was found between the kidney with the higher renin activity and that with the lower activity. However, in group 4, the side, which showed elevated erythropoietin values also had higher renin activity as compared to the essential hypertensive group. Erythropoietin activity probably does not parallel the increased renin activity found in renovascular hypertension or in some cases of non-renovascular hypertension. However, in several cases of renal vascular alterations, both systems can be activated simultaneously.", "contents": "Activity of erythropoietin and renin in renal venous blood of hypertensive patients. Plasma activities of renin and erythropoietin were determined in the renal veins of the right and left kidneys of hypertensive patients. The patients were divided into the following groups either according to the origin of the hypertension (group 1: control for essential hypertension, group 2: renovascular hypertension) or according to the hormone levels (group 3: renin activity exceeding 10 ng/litre in at least one of the renal veins and group 4: erythropoietin activity higher than 4% 59Fe incorporation in at least one of the renal veins). In groups 1, 2 and 3 a statistically significant difference in renin activity was found between the kidney with the higher renin activity and that with the lower activity. However, in group 4, the side, which showed elevated erythropoietin values also had higher renin activity as compared to the essential hypertensive group. Erythropoietin activity probably does not parallel the increased renin activity found in renovascular hypertension or in some cases of non-renovascular hypertension. However, in several cases of renal vascular alterations, both systems can be activated simultaneously.", "PMID": 954803}
{"id": "pubmed23n0032_1346", "title": "Influence of bile acids upon biliary cholesterol and phospholipid secretion in the dog.", "content": "The relationship between taurocholate or taurochenodeoxycholate secretion and cholesterol and phospholipid secretion in bile was studied in anaesthetized dogs infused with graded doses of these bile acids. Cholesterol output was linearly related to bile acid output during both taurocholate and taurochenodeoxycholate infusions. On the average 1.45 nmol and 4.70 nmol of cholesterol were secreted per mumol of taurocholate or taurochenodeoxycholate respectively (P less than 0.001). A linear relationship was also observed between phospholipid secretion rate and taurocholate or taurochenodeoxycholate secretion rates. However, the amounts of phospholipid secreted per mumol of taurocholate or taurochenodeoxycholate (respectively 0.10 and 0.15 mumol) were not significantly different. Thus, bile was less saturated with cholesterol during taurocholate infusions than during taurochenodeoxycholate infusions. These results are consistent with the hypothesis that micelle formation by bile acids is a major determinant of cholesterol and phospholipid secretion in the dog.", "contents": "Influence of bile acids upon biliary cholesterol and phospholipid secretion in the dog. The relationship between taurocholate or taurochenodeoxycholate secretion and cholesterol and phospholipid secretion in bile was studied in anaesthetized dogs infused with graded doses of these bile acids. Cholesterol output was linearly related to bile acid output during both taurocholate and taurochenodeoxycholate infusions. On the average 1.45 nmol and 4.70 nmol of cholesterol were secreted per mumol of taurocholate or taurochenodeoxycholate respectively (P less than 0.001). A linear relationship was also observed between phospholipid secretion rate and taurocholate or taurochenodeoxycholate secretion rates. However, the amounts of phospholipid secreted per mumol of taurocholate or taurochenodeoxycholate (respectively 0.10 and 0.15 mumol) were not significantly different. Thus, bile was less saturated with cholesterol during taurocholate infusions than during taurochenodeoxycholate infusions. These results are consistent with the hypothesis that micelle formation by bile acids is a major determinant of cholesterol and phospholipid secretion in the dog.", "PMID": 954804}
{"id": "pubmed23n0032_1347", "title": "Removal of exogenous plasma triglycerides in forearm muscle and subcutaneous tissue of hyper-and normotriglyceridaemic men.", "content": "Absolute arterio-venous removal and fractional removal of blood-borne exogenous triglyceride particles by skeletal and subcutaneous tissues were determined in eight normotriglyceridaemic and six hypertriglyceridaemic men. Estimations were made by simultaneously sampling blood from catheters inserted into a brachial artery, and in the distal direction, into a superficial and deep vein, draining subcutaneous tissue and skeletal muscle respectively. During the infusion of the fat emulsion IntralipidR for 3 hours steady arterial concentrations of exogenous TG particles were found, indicating first order kinetics. In all subjects absolute arterio-venous and fractional removal of triglycerides were about twice as high in skeletal muscle as in subcutaneous tissue. In skeletal muscle the fractional removal and absolute removal were significantly higher in normotriglyceridaemic than in hypertriglyceridaemic subjects (p less than 0.001). No decrease in removal with time was observed, which would have suggested saturation of removal sites. Mean fractional removal in skeletal muscle was significantly correlated with the k2 value determined by an intravenous fat tolerance test before the infusion study (r = 0.61, p less than 0.05). In subcutaneous tissue fractional removal was also significantly higher in normotriglyceridaemic subjects (p less than 0.05). The results suggest that in both skeletal muscle and subcutaneous tissue the removal system is impaired in hypertriglyceridaemic subjects. This finding supports the concept that a decreased elimination capacity in these tissues may partly account for triglyceride elevation in hypertriglyceridaemic subjects.", "contents": "Removal of exogenous plasma triglycerides in forearm muscle and subcutaneous tissue of hyper-and normotriglyceridaemic men. Absolute arterio-venous removal and fractional removal of blood-borne exogenous triglyceride particles by skeletal and subcutaneous tissues were determined in eight normotriglyceridaemic and six hypertriglyceridaemic men. Estimations were made by simultaneously sampling blood from catheters inserted into a brachial artery, and in the distal direction, into a superficial and deep vein, draining subcutaneous tissue and skeletal muscle respectively. During the infusion of the fat emulsion IntralipidR for 3 hours steady arterial concentrations of exogenous TG particles were found, indicating first order kinetics. In all subjects absolute arterio-venous and fractional removal of triglycerides were about twice as high in skeletal muscle as in subcutaneous tissue. In skeletal muscle the fractional removal and absolute removal were significantly higher in normotriglyceridaemic than in hypertriglyceridaemic subjects (p less than 0.001). No decrease in removal with time was observed, which would have suggested saturation of removal sites. Mean fractional removal in skeletal muscle was significantly correlated with the k2 value determined by an intravenous fat tolerance test before the infusion study (r = 0.61, p less than 0.05). In subcutaneous tissue fractional removal was also significantly higher in normotriglyceridaemic subjects (p less than 0.05). The results suggest that in both skeletal muscle and subcutaneous tissue the removal system is impaired in hypertriglyceridaemic subjects. This finding supports the concept that a decreased elimination capacity in these tissues may partly account for triglyceride elevation in hypertriglyceridaemic subjects.", "PMID": 954805}
{"id": "pubmed23n0032_1348", "title": "The effects of prostaglandin E1 and sodium meclofenamate on blood pressure in renal hypertensive rats.", "content": "Conflicting evidence exists regarding the ability of PGE1 to normalize blood pressure in renal hypertensive rats. We performed experiments to determine the effect of PGE1 (15 mug/kg i.p. daily for 3 weeks) in renal hypertensive Wistars and found no significant change in systolic pressure. A higher dose (150 mug/kg i.p.) lowered pressure after 14 days of treatment, but not back to control levels. Further investigations are required to establish the mechanism whereby PGE1 evokes this fall. To test the hypothesis that endogenous prostaglandins have a hypotensive function in renal hypertension, experiments were performed using sodium meclofenamate to inhibit prostaglandin biosynthesis. In chronic hypertensive rats the drug had no significant effect, while in the acute phase of renal hypertension there was a dose-dependent inhibition of the pressure rise. The possibility is suggested that prostaglandins may initiate or sustain the acute phase of renal hypertension in rats.", "contents": "The effects of prostaglandin E1 and sodium meclofenamate on blood pressure in renal hypertensive rats. Conflicting evidence exists regarding the ability of PGE1 to normalize blood pressure in renal hypertensive rats. We performed experiments to determine the effect of PGE1 (15 mug/kg i.p. daily for 3 weeks) in renal hypertensive Wistars and found no significant change in systolic pressure. A higher dose (150 mug/kg i.p.) lowered pressure after 14 days of treatment, but not back to control levels. Further investigations are required to establish the mechanism whereby PGE1 evokes this fall. To test the hypothesis that endogenous prostaglandins have a hypotensive function in renal hypertension, experiments were performed using sodium meclofenamate to inhibit prostaglandin biosynthesis. In chronic hypertensive rats the drug had no significant effect, while in the acute phase of renal hypertension there was a dose-dependent inhibition of the pressure rise. The possibility is suggested that prostaglandins may initiate or sustain the acute phase of renal hypertension in rats.", "PMID": 954806}
{"id": "pubmed23n0032_1349", "title": "Effects of levomepromazine, chlorpromazine and their sulfoxides on isolated rat atria.", "content": "The effects of levomepromazine, chlorpromazine and their sulfoxides were studied on spontaneously beating and on electrically driven rat atria in vitro. Levomepromazine, chlorpromazine and levomepromazine sulfoxide produced a dose-dependent decrease in the work index of spontaneously beating atria and in the contractile force of electrically driven atria, while chlorpromazine sulfoxide was relatively inactive in these respects. At higher concentrations, levomepromazine sulfoxide caused a pronounced increase in the threshold for electrical stimulation and the effective refractory period. Compared to chlorpromazine, levomepromazine looses less of its cardio-depressive effect through sulfoxidation.", "contents": "Effects of levomepromazine, chlorpromazine and their sulfoxides on isolated rat atria. The effects of levomepromazine, chlorpromazine and their sulfoxides were studied on spontaneously beating and on electrically driven rat atria in vitro. Levomepromazine, chlorpromazine and levomepromazine sulfoxide produced a dose-dependent decrease in the work index of spontaneously beating atria and in the contractile force of electrically driven atria, while chlorpromazine sulfoxide was relatively inactive in these respects. At higher concentrations, levomepromazine sulfoxide caused a pronounced increase in the threshold for electrical stimulation and the effective refractory period. Compared to chlorpromazine, levomepromazine looses less of its cardio-depressive effect through sulfoxidation.", "PMID": 954807}
{"id": "pubmed23n0032_1350", "title": "Turnover of acid dopamine metabolites in striatal and mesolimbic tissue of the rat brain.", "content": "The subject of this study was the disappearance of 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the brain of rats treated with pargyline and pargyline in combination with tropolone. The DOPAC decline was exponential after pargyline treatment, while the HVA decline became a first order elimination when in addition to monoamine oxidase, catechol-O-methyl transferase was also inhibited. The turnover of the metabolites was calculated by multiplying the steady state level by the fractional rate constant (k). The DOPAC turnover was found to be 23.3 nmol/g/h in the corpus striatum and 22.6 nmol/g/h in the mesolimbic structures. The HVA turnover was 11.2 nmol/g/h in the corpus striatum and 6.7 nmol/g/h in the mesolimbic structures. The data showed that under control conditions DOPAC is only partially O-methylated to HVA, while the formation of HVA via the methoxytyramine pathway is unlikely. DOPAC turnover therefore probably approximates dopamine turnover.", "contents": "Turnover of acid dopamine metabolites in striatal and mesolimbic tissue of the rat brain. The subject of this study was the disappearance of 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the brain of rats treated with pargyline and pargyline in combination with tropolone. The DOPAC decline was exponential after pargyline treatment, while the HVA decline became a first order elimination when in addition to monoamine oxidase, catechol-O-methyl transferase was also inhibited. The turnover of the metabolites was calculated by multiplying the steady state level by the fractional rate constant (k). The DOPAC turnover was found to be 23.3 nmol/g/h in the corpus striatum and 22.6 nmol/g/h in the mesolimbic structures. The HVA turnover was 11.2 nmol/g/h in the corpus striatum and 6.7 nmol/g/h in the mesolimbic structures. The data showed that under control conditions DOPAC is only partially O-methylated to HVA, while the formation of HVA via the methoxytyramine pathway is unlikely. DOPAC turnover therefore probably approximates dopamine turnover.", "PMID": 954808}
{"id": "pubmed23n0032_1351", "title": "Distribution and occurrence of amphetamine and p-hydroxyamphetamine in tissues of the rat after injection of d-amphetamine sulfate.", "content": "The distribution of amphetamine and the formation and distribution of its principal metabolite p-hydroxyamphetamine after the injection of relatively small amounts of d-amphetamine was examined in the rat. Amphetamine entered all organs readily and was rapidly eliminated. p-Hydroxyamphetamine was rapidly synthesised and it is suggested that substrate inhibition of hepatic hydroxylating enzymes occurs. In the brain, amphetamine was homogeneously distributed between the seven brain regions examined whereas p-hydroxyamphetamine was predominantly concentrated in the striatum.", "contents": "Distribution and occurrence of amphetamine and p-hydroxyamphetamine in tissues of the rat after injection of d-amphetamine sulfate. The distribution of amphetamine and the formation and distribution of its principal metabolite p-hydroxyamphetamine after the injection of relatively small amounts of d-amphetamine was examined in the rat. Amphetamine entered all organs readily and was rapidly eliminated. p-Hydroxyamphetamine was rapidly synthesised and it is suggested that substrate inhibition of hepatic hydroxylating enzymes occurs. In the brain, amphetamine was homogeneously distributed between the seven brain regions examined whereas p-hydroxyamphetamine was predominantly concentrated in the striatum.", "PMID": 954809}
{"id": "pubmed23n0032_1352", "title": "The masking role of the sodium pump and chloride ions on the effect of carbachol in- and outside the endplate region of rat diaphragm muscle.", "content": "The effect of carbachol (10(-3)M) on the membrane potential of rat diaphragm muscle fibres near and remote from the endplate has been investigated by means of a microelectrode technique. In the endplate region a rather slow depolarization was observed followed by spontaneous repolarization. Outside the endplate region similar, but smaller, responses could be measured. In a muscle part separated from the endplate zone, carbachol had no depolarizing effect. Inhibition of the sodium pump by ouabain (10(-4)M) or potassium-free medium not changing the membrane potential itself, allowed carbachol to exert a depolarizing effect on this endplate-free membrane, while in endplate-free regions continuous with the endplate-zone the depolarizations already observed were enhanced. In low chloride solutions carbachol also had a depolarizing effect on endplate-free muscle membrane. It is concluded from these experiments that a direct effect of cholinergic drugs on endplate-free muscle membrane of rat diaphragm is counteracted by the sodium pump in combination with passive chloride movements. The results are discussed in the context of known receptor densities and chloride effects.", "contents": "The masking role of the sodium pump and chloride ions on the effect of carbachol in- and outside the endplate region of rat diaphragm muscle. The effect of carbachol (10(-3)M) on the membrane potential of rat diaphragm muscle fibres near and remote from the endplate has been investigated by means of a microelectrode technique. In the endplate region a rather slow depolarization was observed followed by spontaneous repolarization. Outside the endplate region similar, but smaller, responses could be measured. In a muscle part separated from the endplate zone, carbachol had no depolarizing effect. Inhibition of the sodium pump by ouabain (10(-4)M) or potassium-free medium not changing the membrane potential itself, allowed carbachol to exert a depolarizing effect on this endplate-free membrane, while in endplate-free regions continuous with the endplate-zone the depolarizations already observed were enhanced. In low chloride solutions carbachol also had a depolarizing effect on endplate-free muscle membrane. It is concluded from these experiments that a direct effect of cholinergic drugs on endplate-free muscle membrane of rat diaphragm is counteracted by the sodium pump in combination with passive chloride movements. The results are discussed in the context of known receptor densities and chloride effects.", "PMID": 954810}
{"id": "pubmed23n0032_1353", "title": "Effects of anesthetics and septal lesions and stimulation on 3H-acetylcholine formation in rat hippocampus.", "content": "The rate of 3H-acetylcholine (ACh) formation from i.v. injected 3H-choline was studied in the rat hippocampus after various treatments which alter the activity of cholinergic septal hippocampal neurons. Administration of pentobarbital and placement of acute septal lesion reduced the formation of 3H-ACh but did not change 3H-choline content. Chloral hydrate administration reduced the formation of 3H-ACh and also increased 3H-choline content. The chloral hydrate induced increase in 3H-choline occurred also in animals with chronic septal lesions. Electrical stimulation of the septum caused an increase in both 3H-ACh and 3/-choline. Chronic septal lesion caused a reduction in both radioactive and endogenous ACh, but did not affect radioactive or endogenous choline. These findings suggest that there are multiple pools of choline in the brain and that the precursor pool for ACh synthesis is difficult to measure. Overall, the parameter that best correlated with cholinergic activity was the level of 3H-ACh. Possible mechanisms, for this finding are discussed.", "contents": "Effects of anesthetics and septal lesions and stimulation on 3H-acetylcholine formation in rat hippocampus. The rate of 3H-acetylcholine (ACh) formation from i.v. injected 3H-choline was studied in the rat hippocampus after various treatments which alter the activity of cholinergic septal hippocampal neurons. Administration of pentobarbital and placement of acute septal lesion reduced the formation of 3H-ACh but did not change 3H-choline content. Chloral hydrate administration reduced the formation of 3H-ACh and also increased 3H-choline content. The chloral hydrate induced increase in 3H-choline occurred also in animals with chronic septal lesions. Electrical stimulation of the septum caused an increase in both 3H-ACh and 3/-choline. Chronic septal lesion caused a reduction in both radioactive and endogenous ACh, but did not affect radioactive or endogenous choline. These findings suggest that there are multiple pools of choline in the brain and that the precursor pool for ACh synthesis is difficult to measure. Overall, the parameter that best correlated with cholinergic activity was the level of 3H-ACh. Possible mechanisms, for this finding are discussed.", "PMID": 954811}
{"id": "pubmed23n0032_1354", "title": "Inhibition by ethanol of noradrenaline output from peripheral sympathetic nerves: possible interaction of ethanol with neuronal receptors.", "content": "The sympathetic nerve terminals of the isolated rabbit heart perfused with Tyrode solution were used to study the action of ethanol on the noradrenaline uptake and release. The uptake of exogenous noradrenaline (10 ng/ml) into the sympathetic nerve endings, the noradrenaline output evoked by raising the concentration of potassium ions in the perfusion fluid, and the release in response to electrical stimulation of the nerve axons were inhibited only by lethal concentrations of the alcohol; the concentrations which caused 50% inhibition (IC50) amounted to 760 mM, 830mM, and 1150 mM respectively. However, ethanol at concentrations compatible with moderate intoxication reduced the noradrenaline release in response to activation of the nicotine receptors on the nerve terminals by dimethylphenylpiperazine; the threshold concentration was 36 mM and the IC50 was 129 mM. It is suggested that this effect is due to hydrophobic interaction of the alcohol with receptor proteins, thus inhibiting stimulus formation.", "contents": "Inhibition by ethanol of noradrenaline output from peripheral sympathetic nerves: possible interaction of ethanol with neuronal receptors. The sympathetic nerve terminals of the isolated rabbit heart perfused with Tyrode solution were used to study the action of ethanol on the noradrenaline uptake and release. The uptake of exogenous noradrenaline (10 ng/ml) into the sympathetic nerve endings, the noradrenaline output evoked by raising the concentration of potassium ions in the perfusion fluid, and the release in response to electrical stimulation of the nerve axons were inhibited only by lethal concentrations of the alcohol; the concentrations which caused 50% inhibition (IC50) amounted to 760 mM, 830mM, and 1150 mM respectively. However, ethanol at concentrations compatible with moderate intoxication reduced the noradrenaline release in response to activation of the nicotine receptors on the nerve terminals by dimethylphenylpiperazine; the threshold concentration was 36 mM and the IC50 was 129 mM. It is suggested that this effect is due to hydrophobic interaction of the alcohol with receptor proteins, thus inhibiting stimulus formation.", "PMID": 954812}
{"id": "pubmed23n0032_1355", "title": "A new probe for heterogeneity in muscarinic receptors: 2-methyl-spiro-(1, 3-dioxolane-4, 3')-quinuclidine.", "content": "The title compound (MSDQ) is a new muscarinic agonist related to 3-acetoxyquinuclidine (3-AcQ) but also of a highly rigid structure. In view of this, it may constitute a probe for the detection of heterogeneity among muscarinic receptors. Indeed, equipotent molar ratios (EPMR) for ACh, 3-AcQ and MSDQ were as follows: guinea pig ileum, 1 : 14 : 240; vasodepressor effect in the cat, 1 : 6 : 188. But EPMR for 3-AcQ and MSDQ as stimulants of the superior cervical ganglion in the cat were 1 : 1 and for the induction of tremors in mice, 9 : 5. No such subtle differences in receptor specificity were detected when the probe used was the 2, 2-diphenyl analogue (DiPSDQ) of MSDQ which was a powerful competitive antagonist in all systems, more potent than atropine, but with a CNS/PNS activity of 1.1 compared to 26 for atropine. In view of this, the use of potent antagonists as probes for muscarinic receptor heterogeneity is questionable.", "contents": "A new probe for heterogeneity in muscarinic receptors: 2-methyl-spiro-(1, 3-dioxolane-4, 3')-quinuclidine. The title compound (MSDQ) is a new muscarinic agonist related to 3-acetoxyquinuclidine (3-AcQ) but also of a highly rigid structure. In view of this, it may constitute a probe for the detection of heterogeneity among muscarinic receptors. Indeed, equipotent molar ratios (EPMR) for ACh, 3-AcQ and MSDQ were as follows: guinea pig ileum, 1 : 14 : 240; vasodepressor effect in the cat, 1 : 6 : 188. But EPMR for 3-AcQ and MSDQ as stimulants of the superior cervical ganglion in the cat were 1 : 1 and for the induction of tremors in mice, 9 : 5. No such subtle differences in receptor specificity were detected when the probe used was the 2, 2-diphenyl analogue (DiPSDQ) of MSDQ which was a powerful competitive antagonist in all systems, more potent than atropine, but with a CNS/PNS activity of 1.1 compared to 26 for atropine. In view of this, the use of potent antagonists as probes for muscarinic receptor heterogeneity is questionable.", "PMID": 954813}
{"id": "pubmed23n0032_1356", "title": "Alteration of tumor growth by aspirin and indomethacin: studies with two transplantable tumors in mouse.", "content": "Oral daily administration of aspirin or indomethacin retarded growth of experimental tumors in mouse. Aspirin treatment, 150 mg/kg twice daily, inhibited growth of a transplantable mast-cell ascites tumor (P815) by 39-43% (p less than 0.001) and of a s.c. transplanted Lewis lung carcinoma by 52% (p less than 0.025) without adversely affecting body growth. The total serotonin, histamine and histidine decarboxylase content of the ascites tumor was also reduced as was the urinary excretion of the amines. Treatment with 3 and 5 mg/kg indomethacin resulted in 40% (p less than 0.01) and 80% (p less than 0.001) reduction, respectively, in ascites tumor growth. With the higher dose of indomethacin, no tumor was observed in half of the animals inoculated with tumor, although signs of indomethacin toxicity (reduced body growth, gastric lesion) was evident in the animals.", "contents": "Alteration of tumor growth by aspirin and indomethacin: studies with two transplantable tumors in mouse. Oral daily administration of aspirin or indomethacin retarded growth of experimental tumors in mouse. Aspirin treatment, 150 mg/kg twice daily, inhibited growth of a transplantable mast-cell ascites tumor (P815) by 39-43% (p less than 0.001) and of a s.c. transplanted Lewis lung carcinoma by 52% (p less than 0.025) without adversely affecting body growth. The total serotonin, histamine and histidine decarboxylase content of the ascites tumor was also reduced as was the urinary excretion of the amines. Treatment with 3 and 5 mg/kg indomethacin resulted in 40% (p less than 0.01) and 80% (p less than 0.001) reduction, respectively, in ascites tumor growth. With the higher dose of indomethacin, no tumor was observed in half of the animals inoculated with tumor, although signs of indomethacin toxicity (reduced body growth, gastric lesion) was evident in the animals.", "PMID": 954814}
{"id": "pubmed23n0032_1357", "title": "Studies on the site of action of clonidine utilizing a sympathetic-cholinergic system.", "content": "Clonidine (30 mug/kg, i.v.) reduced centrally evoked electrodermal responses (EDR) following stimulation of reactive loci in the hypothalamus and medulla. The responses were most depressed following low frequency stimulation. Similar results were observed on the EDR evoked by stimulation of the cervical cord in the spinal cat. Little effect was seen following peripheral nerve stimulation. These results demonstrate that clonidine depresses the reactivity of this sympathetic-cholinergic system at all central levels including the cervical cord.", "contents": "Studies on the site of action of clonidine utilizing a sympathetic-cholinergic system. Clonidine (30 mug/kg, i.v.) reduced centrally evoked electrodermal responses (EDR) following stimulation of reactive loci in the hypothalamus and medulla. The responses were most depressed following low frequency stimulation. Similar results were observed on the EDR evoked by stimulation of the cervical cord in the spinal cat. Little effect was seen following peripheral nerve stimulation. These results demonstrate that clonidine depresses the reactivity of this sympathetic-cholinergic system at all central levels including the cervical cord.", "PMID": 954815}
{"id": "pubmed23n0032_1358", "title": "Effects of chlorprothixene isomers on platelet 5-hydroxytryptamine receptors: evidence for different 5-hydroxytryptamine conformations at uptake and stimulatory sites.", "content": "The thioxanthene neuroleptic, cis-chlorprothixene, was approximately 200 times more potent than its transisomer as an inhibitor of the aggregation of human blood platelets induced by 5-hydroxytryptamine (5HT). Against the active uptake of 5HT by these cells, however, trans-chlorprothixene was twice as inhibitory as its cisisomer, and this inhibition was found to be competitive. It is suggested that 5HT adopts different conformations for binding to its two platelet receptors.", "contents": "Effects of chlorprothixene isomers on platelet 5-hydroxytryptamine receptors: evidence for different 5-hydroxytryptamine conformations at uptake and stimulatory sites. The thioxanthene neuroleptic, cis-chlorprothixene, was approximately 200 times more potent than its transisomer as an inhibitor of the aggregation of human blood platelets induced by 5-hydroxytryptamine (5HT). Against the active uptake of 5HT by these cells, however, trans-chlorprothixene was twice as inhibitory as its cisisomer, and this inhibition was found to be competitive. It is suggested that 5HT adopts different conformations for binding to its two platelet receptors.", "PMID": 954816}
{"id": "pubmed23n0032_1359", "title": "The positive inotropic and chronotropic actions of some amino acids and their effects in mobilizing noradrenaline.", "content": "The effects of methionine, cysteine and serine on the rate and strength of contractions of isolated Langendorff preparations of perfused dog heart and on spontaneous release of catecholamine (CA) from these hearts were investigated. These neutral amino acids increased the amplitude of ventricular contractions. Methionine and cysteine also stimulated the ventricular rate. They induced release of noradrenaline (NA) from the isolated heart. Their inotropic and chronotropic effects were significantly inhibited by pretreatment of the heart with propranolol.", "contents": "The positive inotropic and chronotropic actions of some amino acids and their effects in mobilizing noradrenaline. The effects of methionine, cysteine and serine on the rate and strength of contractions of isolated Langendorff preparations of perfused dog heart and on spontaneous release of catecholamine (CA) from these hearts were investigated. These neutral amino acids increased the amplitude of ventricular contractions. Methionine and cysteine also stimulated the ventricular rate. They induced release of noradrenaline (NA) from the isolated heart. Their inotropic and chronotropic effects were significantly inhibited by pretreatment of the heart with propranolol.", "PMID": 954817}
{"id": "pubmed23n0032_1360", "title": "Cardiovascular responses to acute hypoxia in dogs pretreated with benserazide and L-DOPA.", "content": "In the control dogs, hypoxia induced different hemodynamic modifications (i.e. an increase of arterial blood pressure, heart rate, cardiac output, regional blood flow) which seem to be related to a release of epinephrine. Pretreatment with benserazide (20 mg/kg i.v.) and L-Dopa (50 mg/kg i.p.) either lessened or completely suppressed any sign of sympathetic stimulation. These results suggest that chemoreceptors are centrally inhibited as had been previously proved for baroreceptors.", "contents": "Cardiovascular responses to acute hypoxia in dogs pretreated with benserazide and L-DOPA. In the control dogs, hypoxia induced different hemodynamic modifications (i.e. an increase of arterial blood pressure, heart rate, cardiac output, regional blood flow) which seem to be related to a release of epinephrine. Pretreatment with benserazide (20 mg/kg i.v.) and L-Dopa (50 mg/kg i.p.) either lessened or completely suppressed any sign of sympathetic stimulation. These results suggest that chemoreceptors are centrally inhibited as had been previously proved for baroreceptors.", "PMID": 954818}
{"id": "pubmed23n0032_1361", "title": "Recovery of central respiratory function following anticholinesterase intoxication.", "content": "Spontaneous recovery of central respiratory function was studied in anesthetized guinea pigs intoxicated with pinacolyl methylphosphonofluoridate (Soman) or isopropyl methylphosphonofluoridate (Sarin). I.v adiministration of either agent produced an immediate disruption phrenic nerve activiity and resulting ventilatory failure. Animals were maintained on artifical respiration until spontaneous functional recovery was complete, as evidenced by the re-establishment of synchronized burst activity on the phrenic nerve and return of tracheal airflow. This usually occurred within 1 h. Animals were sacrificed at predetermined intervals after intoxication, and the brainstem homogenates were analyzed for AChE activity. Results showed no significant return of AChE activity after 1 h, although functional recovery of respiration was complete within this time. Additional doses of the agents were administered at various times after recovery from the respiratory blockade. Following spontaneous restoration of ventilatory function, subsequent injections of the organophosphorus compounds failed to reinstate central respiratory paralysis, although they further depressed brainstem AChE levels. These data suggest that spontaneous recovery of central respiratory function after intoxication with Soman or Sarin may not be related to the return of AChE activity.", "contents": "Recovery of central respiratory function following anticholinesterase intoxication. Spontaneous recovery of central respiratory function was studied in anesthetized guinea pigs intoxicated with pinacolyl methylphosphonofluoridate (Soman) or isopropyl methylphosphonofluoridate (Sarin). I.v adiministration of either agent produced an immediate disruption phrenic nerve activiity and resulting ventilatory failure. Animals were maintained on artifical respiration until spontaneous functional recovery was complete, as evidenced by the re-establishment of synchronized burst activity on the phrenic nerve and return of tracheal airflow. This usually occurred within 1 h. Animals were sacrificed at predetermined intervals after intoxication, and the brainstem homogenates were analyzed for AChE activity. Results showed no significant return of AChE activity after 1 h, although functional recovery of respiration was complete within this time. Additional doses of the agents were administered at various times after recovery from the respiratory blockade. Following spontaneous restoration of ventilatory function, subsequent injections of the organophosphorus compounds failed to reinstate central respiratory paralysis, although they further depressed brainstem AChE levels. These data suggest that spontaneous recovery of central respiratory function after intoxication with Soman or Sarin may not be related to the return of AChE activity.", "PMID": 954819}
{"id": "pubmed23n0032_1362", "title": "Des-Asp1-angiotensin I: a metabolite of angiotensin I in the perfused feline adrenal.", "content": "The administration of radioactive angiotensin I to the retrogradely perfused feline adrenal gland caused a brisk discharge of catecholamines. Recovery of the labelled decapeptide and metabolites in the adrenal effluent fluid was complete in 5 min. Radioimmunoassay of this perfusate revealed that most of the peptide remained as angiotensin I, but chromatographic and electrophoretic evaluation indicated that  greater than 68% of the peptide had been metabolized to des-asp1 -angiotensin I. The absence of des-asp1  -angiotensin II, angiotensin II or his-3H-leu in adrenal effluent fluid suggested minimal dipeptidyl carboxypeptidase activity in this preparation. In addition, the profile of angiotensin I metabolites from the perfused adrenal was not altered by treatment with a converting enzyme inhibitor B. jararaca nonapeptide. The des-asp1-angiotensin I peptide was a very weak secretagogue in the adrenal medulla. If metabolism of the decapeptide to the nonapeptide occurs in the medulla, this may represent a pathway to limit the secretory action of angiotensin I. These results suggest a high degree of adrenal aminopeptidase activity which may be primarily localized in the adrenal cortex.", "contents": "Des-Asp1-angiotensin I: a metabolite of angiotensin I in the perfused feline adrenal. The administration of radioactive angiotensin I to the retrogradely perfused feline adrenal gland caused a brisk discharge of catecholamines. Recovery of the labelled decapeptide and metabolites in the adrenal effluent fluid was complete in 5 min. Radioimmunoassay of this perfusate revealed that most of the peptide remained as angiotensin I, but chromatographic and electrophoretic evaluation indicated that  greater than 68% of the peptide had been metabolized to des-asp1 -angiotensin I. The absence of des-asp1  -angiotensin II, angiotensin II or his-3H-leu in adrenal effluent fluid suggested minimal dipeptidyl carboxypeptidase activity in this preparation. In addition, the profile of angiotensin I metabolites from the perfused adrenal was not altered by treatment with a converting enzyme inhibitor B. jararaca nonapeptide. The des-asp1-angiotensin I peptide was a very weak secretagogue in the adrenal medulla. If metabolism of the decapeptide to the nonapeptide occurs in the medulla, this may represent a pathway to limit the secretory action of angiotensin I. These results suggest a high degree of adrenal aminopeptidase activity which may be primarily localized in the adrenal cortex.", "PMID": 954820}
{"id": "pubmed23n0032_1363", "title": "Tolerance to hexobarbital and supersensitivity to pilocarpine after chronic barbital treatments in the rat.", "content": "Tolerance and physical dependence induced by chronic treatments with depressant drugs has been proposed as being due to denervation or disuse supersensitivity in the central nervous system. To illustrate this hypothesis, tolerance to barbiturates and the sensitivity to central cholinergic stimulation were recorded in the same male rats. Tolerance to barbiturates was recorded with a hexobarbital threshold. The dose needed to obtain an EEG criterion was measured. Sensitivity in central cholinergic structures was recorded by a temperature threshold method. A subthreshold dose of pilocarpine (3.5 mg/kg) was given s.c. together with 1.0 mg/kg methylscopolamine i.p. An increase in sensitivity to pilocarpine was recorded as a decrease in body temperature. In the abstinence period after 42 weeks of oral treatment with barbital, tolerance was recorded with the hexobarbital threshold method. The tolerance had two peaks, one after the end of the treatment and one approximately 3 weeks later. An increased sensitivity to pilocarpine was also recorded in the abstinence period. It had the same biphasic time pattern as the tolerance. Cholinergic neurones are thus probably involved in the changes induced by chronic barbital treatment. The denervation supersensitivity hypothesis of tolerance was not refuted by the present experiments.", "contents": "Tolerance to hexobarbital and supersensitivity to pilocarpine after chronic barbital treatments in the rat. Tolerance and physical dependence induced by chronic treatments with depressant drugs has been proposed as being due to denervation or disuse supersensitivity in the central nervous system. To illustrate this hypothesis, tolerance to barbiturates and the sensitivity to central cholinergic stimulation were recorded in the same male rats. Tolerance to barbiturates was recorded with a hexobarbital threshold. The dose needed to obtain an EEG criterion was measured. Sensitivity in central cholinergic structures was recorded by a temperature threshold method. A subthreshold dose of pilocarpine (3.5 mg/kg) was given s.c. together with 1.0 mg/kg methylscopolamine i.p. An increase in sensitivity to pilocarpine was recorded as a decrease in body temperature. In the abstinence period after 42 weeks of oral treatment with barbital, tolerance was recorded with the hexobarbital threshold method. The tolerance had two peaks, one after the end of the treatment and one approximately 3 weeks later. An increased sensitivity to pilocarpine was also recorded in the abstinence period. It had the same biphasic time pattern as the tolerance. Cholinergic neurones are thus probably involved in the changes induced by chronic barbital treatment. The denervation supersensitivity hypothesis of tolerance was not refuted by the present experiments.", "PMID": 954821}
{"id": "pubmed23n0032_1364", "title": "A study of muscarinic receptor heterogeneity with weak antagonists.", "content": "A study of heterogeneity among muscarinic receptors was carried out with new rigid molecules, comprising structures in the fused quinuclidine-valerolactone, quinuclidine-cyclohexenone, quinuclidine-cyclohexanone and quinuclidine-cyclohexane derivatives. These are structurally related to the potent muscarinic agent, 3-acetoxyquinuclidine but substantially different from in it conformation. All proved to antagonize acetylcholine-like activity, but to a different extent in different systems. The equipotent molar ratio with respect to atropine (as 1) was: isolated guinea pig ileum, 10,000-1,000; salivary gland (mouse), 1,000-100; superior cervical ganglion (cat), 100-10; CNS (mouse), approximately 10. It is suggested that the rigid structure induces a three-point constrained fit in the receptor (onium group, hydrophobic moiety and carbonyl group), but that not all muscarinic receptors are capable of responding equally. In this case, receptor specificity of the drug is a direct consequence of its graded departure from the preferred conformation of acetycholine and, therefore, is necessarily associated with partial loss of potency.", "contents": "A study of muscarinic receptor heterogeneity with weak antagonists. A study of heterogeneity among muscarinic receptors was carried out with new rigid molecules, comprising structures in the fused quinuclidine-valerolactone, quinuclidine-cyclohexenone, quinuclidine-cyclohexanone and quinuclidine-cyclohexane derivatives. These are structurally related to the potent muscarinic agent, 3-acetoxyquinuclidine but substantially different from in it conformation. All proved to antagonize acetylcholine-like activity, but to a different extent in different systems. The equipotent molar ratio with respect to atropine (as 1) was: isolated guinea pig ileum, 10,000-1,000; salivary gland (mouse), 1,000-100; superior cervical ganglion (cat), 100-10; CNS (mouse), approximately 10. It is suggested that the rigid structure induces a three-point constrained fit in the receptor (onium group, hydrophobic moiety and carbonyl group), but that not all muscarinic receptors are capable of responding equally. In this case, receptor specificity of the drug is a direct consequence of its graded departure from the preferred conformation of acetycholine and, therefore, is necessarily associated with partial loss of potency.", "PMID": 954822}
{"id": "pubmed23n0032_1365", "title": "Radioimmunoassay for the simultaneous determination of morphine and codeine.", "content": "Antiserum against morphine was produced in rabbits immunized with morphine hapten conjugated to bovine serum albumin. The carrier protein was conjugated to the nitrogen atom of the opiate alkaloid in order to make the phenolic hydroxy group on C3 and the alcoholic group on C6 as determinant groups. The antibody does not recognize codeine or the major metabolite of morphine, 3-O-monoglucuronide. This antibody was used in conjunction with an antibody prepared against 3-O-carboxymethylmorphine to develop a radioimmunoassay which can measure codeine in the presence of morphine. The assay was used to follow both the plasma and brain levels of codeine and its biotransformation to morphine. Codeine when administered at a dose of 5 mg/kg i.v. showed a biphasic plasma decay curve the first phase of which had a +1/2 of 26 min. Peak concentrations of morphine were detected in the plasma following that dose of codeine at 0.5 h. 30 min after the injection of 20 mg/kg i.p. codeine, the brain levels of morphine were only 2% that of codeine. Thereafter, the brain levels of morphine slowly declined.", "contents": "Radioimmunoassay for the simultaneous determination of morphine and codeine. Antiserum against morphine was produced in rabbits immunized with morphine hapten conjugated to bovine serum albumin. The carrier protein was conjugated to the nitrogen atom of the opiate alkaloid in order to make the phenolic hydroxy group on C3 and the alcoholic group on C6 as determinant groups. The antibody does not recognize codeine or the major metabolite of morphine, 3-O-monoglucuronide. This antibody was used in conjunction with an antibody prepared against 3-O-carboxymethylmorphine to develop a radioimmunoassay which can measure codeine in the presence of morphine. The assay was used to follow both the plasma and brain levels of codeine and its biotransformation to morphine. Codeine when administered at a dose of 5 mg/kg i.v. showed a biphasic plasma decay curve the first phase of which had a +1/2 of 26 min. Peak concentrations of morphine were detected in the plasma following that dose of codeine at 0.5 h. 30 min after the injection of 20 mg/kg i.p. codeine, the brain levels of morphine were only 2% that of codeine. Thereafter, the brain levels of morphine slowly declined.", "PMID": 954823}
{"id": "pubmed23n0032_1366", "title": "Influence of chloride and sodium pump activity on carbachol- and acetylaholine-induced depolarizations in denervated rat diphragm.", "content": "The effect was measured of low chloride medium and ouabain on carbachol- and acetylcholine-induced depolarizations in denervated rat diaphragm. Membrane potentials were measured in rat diaphragm muscle fibers 10-14 days after denervation. Depolarizations induced by carbachol and acetycholine were increased when the extracellular chloride concentration was diminished from 110 to 40 or 10 mM. Sodium pump inhibition by ouabain (10(-4) M) dramatically enhanced the carbachol depolarizations. In 40 mM Cl- solutions, dose-response relations were determineed with ACh in the absence of presence of dTc. For ACh a pD2 value of 5.2 was found; the pA2 value for dTC appeared to be about 6.4. Therefore, the binding properties of the post-denervational receptors do not appear to be influenced by the chloride gradient. It was concluded that drug-induced depolarizations of the denervated rat diaphragm are short-circuited by chloride ions and counteracted by sodium pumping.", "contents": "Influence of chloride and sodium pump activity on carbachol- and acetylaholine-induced depolarizations in denervated rat diphragm. The effect was measured of low chloride medium and ouabain on carbachol- and acetylcholine-induced depolarizations in denervated rat diaphragm. Membrane potentials were measured in rat diaphragm muscle fibers 10-14 days after denervation. Depolarizations induced by carbachol and acetycholine were increased when the extracellular chloride concentration was diminished from 110 to 40 or 10 mM. Sodium pump inhibition by ouabain (10(-4) M) dramatically enhanced the carbachol depolarizations. In 40 mM Cl- solutions, dose-response relations were determineed with ACh in the absence of presence of dTc. For ACh a pD2 value of 5.2 was found; the pA2 value for dTC appeared to be about 6.4. Therefore, the binding properties of the post-denervational receptors do not appear to be influenced by the chloride gradient. It was concluded that drug-induced depolarizations of the denervated rat diaphragm are short-circuited by chloride ions and counteracted by sodium pumping.", "PMID": 954824}
{"id": "pubmed23n0032_1367", "title": "Steroid anaesthesia (Althesin - CT 1341) and dorsal horn cell activities in feline spinal cord.", "content": "The effects of steroid anaesthesia (Althesin -CT 1341) on dorsal horn cells (laminae 4 and 5) were studied by extracellular recordings in the spinal cat. I.v. administration of Althesin (0.2 ml/kg) strongly depressed the spontaneous and evoked activities of both types of cells. No differences were found between cells activated by noxious or innocuous stimuli. These results emphasize the fact that the transmission of afferent messages is depressed by various anaesthetics at the level of the first synapses in the CNS. The depressive effects on lamina 5 cells could explain in part the analgesic effects of Althesin.", "contents": "Steroid anaesthesia (Althesin - CT 1341) and dorsal horn cell activities in feline spinal cord. The effects of steroid anaesthesia (Althesin -CT 1341) on dorsal horn cells (laminae 4 and 5) were studied by extracellular recordings in the spinal cat. I.v. administration of Althesin (0.2 ml/kg) strongly depressed the spontaneous and evoked activities of both types of cells. No differences were found between cells activated by noxious or innocuous stimuli. These results emphasize the fact that the transmission of afferent messages is depressed by various anaesthetics at the level of the first synapses in the CNS. The depressive effects on lamina 5 cells could explain in part the analgesic effects of Althesin.", "PMID": 954825}
{"id": "pubmed23n0032_1368", "title": "Effects of apomorphine and of pimozide on renin secretion in the anaesthetized dog.", "content": "This paper presents the results of a study of the receptors involved in renin hypersecretion induced by dopamine. Apomorphine induces renin hypersecretion without significantly modifying the renal blood flow. This renin hypersecretion is inhibited by haloperidol. However, pimozide, a selective dopaminolytic agent, is incapable of inhibiting the renin hypersecretion induced by dopamine. These results provide supplementary evidence for the existence of renal dopaminergic receptors which would appear to differ from the central dopaminergic receptors.", "contents": "Effects of apomorphine and of pimozide on renin secretion in the anaesthetized dog. This paper presents the results of a study of the receptors involved in renin hypersecretion induced by dopamine. Apomorphine induces renin hypersecretion without significantly modifying the renal blood flow. This renin hypersecretion is inhibited by haloperidol. However, pimozide, a selective dopaminolytic agent, is incapable of inhibiting the renin hypersecretion induced by dopamine. These results provide supplementary evidence for the existence of renal dopaminergic receptors which would appear to differ from the central dopaminergic receptors.", "PMID": 954826}
{"id": "pubmed23n0032_1369", "title": "Effects of acute administration of delta9-tetrahydrocannabinol on pulmonary hemodynamics of anesthetized dogs.", "content": "I.v. administration of delta9-THC (2.5 mg/kg) To anesthetized dogs resulted in a decrease in heart rate, pulmonary blood flow (PBF), and a significant increase in pulmonary artery pressure (PAP) and total pulmonary vascular resistance (PVR). The increase in PVR to delta9-THC was significantly reduced by cardiac pacing, and was virtually abolished either by bilateral vagotomy or by pretreatment with hexamethonium. The data indicated the delta9-THC induced elevation of PVR was mediated via reflexogenic mechanisms involving afferent vagi and efferent autonomic pathways.", "contents": "Effects of acute administration of delta9-tetrahydrocannabinol on pulmonary hemodynamics of anesthetized dogs. I.v. administration of delta9-THC (2.5 mg/kg) To anesthetized dogs resulted in a decrease in heart rate, pulmonary blood flow (PBF), and a significant increase in pulmonary artery pressure (PAP) and total pulmonary vascular resistance (PVR). The increase in PVR to delta9-THC was significantly reduced by cardiac pacing, and was virtually abolished either by bilateral vagotomy or by pretreatment with hexamethonium. The data indicated the delta9-THC induced elevation of PVR was mediated via reflexogenic mechanisms involving afferent vagi and efferent autonomic pathways.", "PMID": 954827}
{"id": "pubmed23n0032_1370", "title": "L-alpha-amino-beta-chloropropionic acid hydroxamide: an inhibitor of GABA-lapha-oxoglutarate aminotrarsferase.", "content": "The intramuscular administration of L-alpha-amino-beta-chloropropinonic acid hydroxamide (2 mmol/kg) to mice strongly inhibited the activity of GABA-T, but not GAD, in the brain of the animals. Adminstration of the compound 3 h prior to isonicotinic acid hydrazide treatment significantly delayed the onset of seizures induced by the hydrazide.", "contents": "L-alpha-amino-beta-chloropropionic acid hydroxamide: an inhibitor of GABA-lapha-oxoglutarate aminotrarsferase. The intramuscular administration of L-alpha-amino-beta-chloropropinonic acid hydroxamide (2 mmol/kg) to mice strongly inhibited the activity of GABA-T, but not GAD, in the brain of the animals. Adminstration of the compound 3 h prior to isonicotinic acid hydrazide treatment significantly delayed the onset of seizures induced by the hydrazide.", "PMID": 954828}
{"id": "pubmed23n0032_1371", "title": "Reduced hypotensive effect of clonidine after lesions of the nucleus tractus solitarii in rats.", "content": "Bilateral electrolytic lesion of the area of the nucleus tractus solitarii (NTS) in rats produced a sustained increase in blood pressure and reduced the hypotensive response to a single dose of clonidine (30 mug/kg, i.v.). The same dose of clonidine evoked a much larger drop in blood pressure in another group of rats in which an equialent increase in blood pressure was produced by bilateral section of the vagosympathetic trunks and occlusion of both carotid arteries. It is concluded that the dorsal part of the medulla oblongata in the area of NTS is an important link in the hypotensive action of clonidine.", "contents": "Reduced hypotensive effect of clonidine after lesions of the nucleus tractus solitarii in rats. Bilateral electrolytic lesion of the area of the nucleus tractus solitarii (NTS) in rats produced a sustained increase in blood pressure and reduced the hypotensive response to a single dose of clonidine (30 mug/kg, i.v.). The same dose of clonidine evoked a much larger drop in blood pressure in another group of rats in which an equialent increase in blood pressure was produced by bilateral section of the vagosympathetic trunks and occlusion of both carotid arteries. It is concluded that the dorsal part of the medulla oblongata in the area of NTS is an important link in the hypotensive action of clonidine.", "PMID": 954829}
{"id": "pubmed23n0032_1372", "title": "Inhibitory effect of a caerulein-like peptide on human gastric secretion.", "content": "A synthetic heptapeptide corresponding to the C-terminal heptapeptide of caerulein but characterized by a nor-leucyl residue replacing the methyonyl residue of caerulein, when given by i.v. infusion (2 mug/kg/h), inhibited by 70-80% pentagastrin (4 mug/kg/h)-stimulated gastric secretion...", "contents": "Inhibitory effect of a caerulein-like peptide on human gastric secretion. A synthetic heptapeptide corresponding to the C-terminal heptapeptide of caerulein but characterized by a nor-leucyl residue replacing the methyonyl residue of caerulein, when given by i.v. infusion (2 mug/kg/h), inhibited by 70-80% pentagastrin (4 mug/kg/h)-stimulated gastric secretion...", "PMID": 954830}
{"id": "pubmed23n0032_1373", "title": "Clozapine: reduction of the initial dopamine turnover increase by repeated treatment.", "content": "The levels of homovarillic acid and 3,4-dihydroxyphenylacetic acid in rat c. striatum after acute and 10 day administration of clozapine (30 and 100 mg/kg p.o.), thioridazine (100 mg/kg p.o.), haloperidol (1 mg/kg p.o.), and chlorpromazine (30 mg/kg p.o.), were estimated. With clozapine and haloperidol, the mesolimbic area was also investigated. With all these neuroleptics, the levels of both dopamine metabolites were reduced after a 10 day treatment as compared to acute administration, sometimes almost to control values. With clozapine, however, such a reduction occurred only with the higher dose of 100 mg/kg p.o., acting for a period longer than 24 h. This tolerance phenomenon was also observed with clozapine and haloperidol in the mesolimbic area. We conclude that clozapine is not qualitatively different from classical neuroleptics with respect to development of biochemical tolerance.", "contents": "Clozapine: reduction of the initial dopamine turnover increase by repeated treatment. The levels of homovarillic acid and 3,4-dihydroxyphenylacetic acid in rat c. striatum after acute and 10 day administration of clozapine (30 and 100 mg/kg p.o.), thioridazine (100 mg/kg p.o.), haloperidol (1 mg/kg p.o.), and chlorpromazine (30 mg/kg p.o.), were estimated. With clozapine and haloperidol, the mesolimbic area was also investigated. With all these neuroleptics, the levels of both dopamine metabolites were reduced after a 10 day treatment as compared to acute administration, sometimes almost to control values. With clozapine, however, such a reduction occurred only with the higher dose of 100 mg/kg p.o., acting for a period longer than 24 h. This tolerance phenomenon was also observed with clozapine and haloperidol in the mesolimbic area. We conclude that clozapine is not qualitatively different from classical neuroleptics with respect to development of biochemical tolerance.", "PMID": 954831}
{"id": "pubmed23n0032_1374", "title": "Dehydrobenzperidol and disopyramide in A-V conduction.", "content": "The effects on A-V conduction of dehydrobenzperidol (Droperidol) 0.35 and 1.0 mg/kg i.v.) and disopyramide (1, 5 and 10 mg/kg i.v.) were tested in anesthetized, open chest and paced dogs. His's bundle electrograms were recorded by means of a catheter electrode or by a sutured electrode-bearing plaque in the A-V nodal region. Droperidol at the lower dose did not modify conduction time, while 1.0 mg/kg (3 times the usual clinical dose), prolonged atrial-His conduction without modifying H-V interval. Disopyramide 1 mg/kg caused a non-significant decrease in atrial-His concuction time, while in doses of 5 and 10 mg/kg it prolonged both conduction times (S-H and H-V), due to a predominant direct depressant effect, which is opposite to the atropinic actions of the drug.", "contents": "Dehydrobenzperidol and disopyramide in A-V conduction. The effects on A-V conduction of dehydrobenzperidol (Droperidol) 0.35 and 1.0 mg/kg i.v.) and disopyramide (1, 5 and 10 mg/kg i.v.) were tested in anesthetized, open chest and paced dogs. His's bundle electrograms were recorded by means of a catheter electrode or by a sutured electrode-bearing plaque in the A-V nodal region. Droperidol at the lower dose did not modify conduction time, while 1.0 mg/kg (3 times the usual clinical dose), prolonged atrial-His conduction without modifying H-V interval. Disopyramide 1 mg/kg caused a non-significant decrease in atrial-His concuction time, while in doses of 5 and 10 mg/kg it prolonged both conduction times (S-H and H-V), due to a predominant direct depressant effect, which is opposite to the atropinic actions of the drug.", "PMID": 954832}
{"id": "pubmed23n0032_1375", "title": "5-Hydroxytryptamine as a sensitizer of somatic nociceptors for pain-producing substances.", "content": "Experiments were performed on loosely restrained, conscious dogs in the femoral artery of which a chronic catheter was implanted. All substances were administered through the catheter and vocalization responses to them were used as a principal measure of nociception. In 10 experiments done on 7 dogs, after a single injection of 3 or 10 mumol of 5-hydroxytryptamine, threshold doses (about 0.5 mumol) of acetylcholine for vocalization produced vocalization responses comparable with those to about 3 times threshold doses. In 5 experiments on 5 dogs, after 10 mumol of 5-hydroxytryptamine, threshold doses (about 0.3 nmol) of bradykinin for vocalization produced vocalization responses comparable with those to about twice threshold doses. These doses of 5-hydroxytryptamine produced small vocalization responses in only 3 of the 12 dogs. These results indicate that 5-hydroxytryptamine, although almost devoid of the activity producing noceceptive responses, sensitizes somatic nociceptors for pain-producing substances.", "contents": "5-Hydroxytryptamine as a sensitizer of somatic nociceptors for pain-producing substances. Experiments were performed on loosely restrained, conscious dogs in the femoral artery of which a chronic catheter was implanted. All substances were administered through the catheter and vocalization responses to them were used as a principal measure of nociception. In 10 experiments done on 7 dogs, after a single injection of 3 or 10 mumol of 5-hydroxytryptamine, threshold doses (about 0.5 mumol) of acetylcholine for vocalization produced vocalization responses comparable with those to about 3 times threshold doses. In 5 experiments on 5 dogs, after 10 mumol of 5-hydroxytryptamine, threshold doses (about 0.3 nmol) of bradykinin for vocalization produced vocalization responses comparable with those to about twice threshold doses. These doses of 5-hydroxytryptamine produced small vocalization responses in only 3 of the 12 dogs. These results indicate that 5-hydroxytryptamine, although almost devoid of the activity producing noceceptive responses, sensitizes somatic nociceptors for pain-producing substances.", "PMID": 954833}
{"id": "pubmed23n0032_1376", "title": "Nicotine-induced weight loss in rats without an effect on appetite.", "content": "Rats were administered 0.8 mg/kg nicotine (as base) i.p. 2 or 3 times a day for 5 weeks, and their mean body weights were observed to be significantly lower than those of control groups administered 0.9% saline on a similar regimen. The reduction in body weight after chronic nicotine administration proved highly replicable and was produced in the absence of a significant decrease in food consumption. Upon cessation of nicotine administration, the mean body weights of the rats returned to control group levels. Administration of phenylbenzoquinone, an agent that stimulated sensory pain receptors, failed to produce a similar decrease in body weight. Likewise, pretreatment with cholinergic blocking drugs, mecamylamine and hexamethonium did not antagonize the nicotine-induced decrease in body weight. Application of these results to the human smoking habit and possible mechanisms of actions are discussed.", "contents": "Nicotine-induced weight loss in rats without an effect on appetite. Rats were administered 0.8 mg/kg nicotine (as base) i.p. 2 or 3 times a day for 5 weeks, and their mean body weights were observed to be significantly lower than those of control groups administered 0.9% saline on a similar regimen. The reduction in body weight after chronic nicotine administration proved highly replicable and was produced in the absence of a significant decrease in food consumption. Upon cessation of nicotine administration, the mean body weights of the rats returned to control group levels. Administration of phenylbenzoquinone, an agent that stimulated sensory pain receptors, failed to produce a similar decrease in body weight. Likewise, pretreatment with cholinergic blocking drugs, mecamylamine and hexamethonium did not antagonize the nicotine-induced decrease in body weight. Application of these results to the human smoking habit and possible mechanisms of actions are discussed.", "PMID": 954834}
{"id": "pubmed23n0032_1377", "title": "Dog platelets fail to aggregate when they form aggregating substances upon stimulation with arachidonic acid.", "content": "Dog platelets are refractory to aggregation by arachidonic acid (AA) but generate an unstable activity that aggregates rabbit platelets. Formation of this activity is inhibited by indomethacin, by the peroxide scavenging enzyme catalase, by two chelating agents that bind Cu+ and Cu2+ ions, by the -SH agent dithiothreitol and is stimulated by cysteine. Agitation of dog platelets is followed by spontaneous aggregation and uncovers aggregation by AA, which is blocked by indomethacin. Neither indomethacin nor apyrase prevent spontaneous aggregation, ruling out both activation of prostaglandin synthetase and leakage of ADP as possible explanations. Complexation of plasma Ca2+ by citrate as an explanation for refractoriness to AA was ruled out by replacing citrate with heparin. Dog platelets are also refractory to PGH2 formed from AA by the cyclo oxygenase component of prostaglandin synthetase. Aggregation of rabbit platelets by PGH2 is not inhibited by indomethacin, by catalase, by dithiothreitol or by metal chelating agents and is not potentiated by cysteine. This confirms that the reagents act before PGH2 is formed. Aggregating activity generated by dog platelets is probably due to an unstable lipoperoxide whose generation involves mechanisms similar to those responsible for aggregation of rabbit platelets, since similar antagonists block both processes.", "contents": "Dog platelets fail to aggregate when they form aggregating substances upon stimulation with arachidonic acid. Dog platelets are refractory to aggregation by arachidonic acid (AA) but generate an unstable activity that aggregates rabbit platelets. Formation of this activity is inhibited by indomethacin, by the peroxide scavenging enzyme catalase, by two chelating agents that bind Cu+ and Cu2+ ions, by the -SH agent dithiothreitol and is stimulated by cysteine. Agitation of dog platelets is followed by spontaneous aggregation and uncovers aggregation by AA, which is blocked by indomethacin. Neither indomethacin nor apyrase prevent spontaneous aggregation, ruling out both activation of prostaglandin synthetase and leakage of ADP as possible explanations. Complexation of plasma Ca2+ by citrate as an explanation for refractoriness to AA was ruled out by replacing citrate with heparin. Dog platelets are also refractory to PGH2 formed from AA by the cyclo oxygenase component of prostaglandin synthetase. Aggregation of rabbit platelets by PGH2 is not inhibited by indomethacin, by catalase, by dithiothreitol or by metal chelating agents and is not potentiated by cysteine. This confirms that the reagents act before PGH2 is formed. Aggregating activity generated by dog platelets is probably due to an unstable lipoperoxide whose generation involves mechanisms similar to those responsible for aggregation of rabbit platelets, since similar antagonists block both processes.", "PMID": 954835}
{"id": "pubmed23n0032_1378", "title": "Different chronotropic and inotropic effects of pentobarbital in the blood-perfused, isolated dog atrium.", "content": "The isolated right atrium of the dog heart was perfused with arterial blood introduced from a carotid artery of a support dog. The selective administration of pentobarbital into the cannulated sinus node artery induced a negative inotropic effect without any significant change in chronotropism at relatively small doses. At higher doses, pentobarbital caused both negative chronotropic and inotropic effects. Even at that time, the negative chronotropic response to pentobarbital was much less than the negative inotropic response. Accordingly, pentobarbital could show a dissociation of electrical and mechanical activity in this preparation. On the other hand, either verapamil or manganese caused a negative chronotropic effect at the threshold dose for inducing a negative inotropic effect. It is suggested that the mode of cardiac action of pentobarbital may differ from calcium antagonists such as manganese or verapamil, although pentobarbital-induced effects may be associated with an impaired removal of calcium ion.", "contents": "Different chronotropic and inotropic effects of pentobarbital in the blood-perfused, isolated dog atrium. The isolated right atrium of the dog heart was perfused with arterial blood introduced from a carotid artery of a support dog. The selective administration of pentobarbital into the cannulated sinus node artery induced a negative inotropic effect without any significant change in chronotropism at relatively small doses. At higher doses, pentobarbital caused both negative chronotropic and inotropic effects. Even at that time, the negative chronotropic response to pentobarbital was much less than the negative inotropic response. Accordingly, pentobarbital could show a dissociation of electrical and mechanical activity in this preparation. On the other hand, either verapamil or manganese caused a negative chronotropic effect at the threshold dose for inducing a negative inotropic effect. It is suggested that the mode of cardiac action of pentobarbital may differ from calcium antagonists such as manganese or verapamil, although pentobarbital-induced effects may be associated with an impaired removal of calcium ion.", "PMID": 954836}
{"id": "pubmed23n0032_1379", "title": "Reduction by indomethacin of furosemide effects in the rabbit.", "content": "The effects of furosemide on urine flow, sodium and potassium excretion and on plasma renin activity (PRA) were studied in anesthetized rabbits with and without pretreatment with indomethacin 5 mg/kg. Furosemide caused a 10-fold increase in urine flow and in sodium excretion, and a 2-3 fold increase in PRA. Pretreatment with the prostaglandin synthesis inhibitor, indomethacin, reduced the effects of furosemide on diuresis and on electrolyte excretion by over 80% (p less than 0.01) and PRA did not increase over the initial level. The results suggest that the effects of furosemide on PRA and on urinary sodium excretion may be related to the intrarenal activation of the prostaglandin system.", "contents": "Reduction by indomethacin of furosemide effects in the rabbit. The effects of furosemide on urine flow, sodium and potassium excretion and on plasma renin activity (PRA) were studied in anesthetized rabbits with and without pretreatment with indomethacin 5 mg/kg. Furosemide caused a 10-fold increase in urine flow and in sodium excretion, and a 2-3 fold increase in PRA. Pretreatment with the prostaglandin synthesis inhibitor, indomethacin, reduced the effects of furosemide on diuresis and on electrolyte excretion by over 80% (p less than 0.01) and PRA did not increase over the initial level. The results suggest that the effects of furosemide on PRA and on urinary sodium excretion may be related to the intrarenal activation of the prostaglandin system.", "PMID": 954837}
{"id": "pubmed23n0032_1380", "title": "Binding of 14C-imipramine by pigmented and non-pigmented tissues.", "content": "When pigmented and non-pigmented rabbit irides were incubated with various concentrations of 14C-imipramine at equilibrium (120 min), the accumulation of the drug by the pigmented iris was 1.5 times as great as that by the non-pigmented iris. The accumulated drug is lost from both types of irides in a complex fashion. However, even after 120 min of washing, the differences in accumulation remain nearly constant. When accumulation of the drug in the non-pigmented iris was analyzed by discontinuous sucrose density gradient, it was observed that the drug was bound mainly by the low density sucrose fractions where the synaptosomes separate. On the contrary, in the pigmented iris approximately 70% of the drug was found in the melanin-containing fraction. The homogenate from the substantia nigra accumulated 1.5 times more than that from the human brain cortex. The affinity of the drug for bovine iris melanin granules and the synthetic L-dopa melanin was 9.9 X 10(5) M-1 and 3.8 X 10(3) M-1, respectively. On the rabbit iris sphincter muscles, imipramine was evaluated for antimuscarinic effects. The apparent dissociation constants, KB values, for the antagonist in the non-pigmented and pigmented iris were 1.7 X 10(-7) M and 3.8 X 10(-6) M, respectively. The low antimuscarinic activity in the pigmented iris is attributed to the loss of the drug to the pigment. On this basis, relevancy of the drug binding by pigmented tissues to the effects of this tricyclic drug is discussed.", "contents": "Binding of 14C-imipramine by pigmented and non-pigmented tissues. When pigmented and non-pigmented rabbit irides were incubated with various concentrations of 14C-imipramine at equilibrium (120 min), the accumulation of the drug by the pigmented iris was 1.5 times as great as that by the non-pigmented iris. The accumulated drug is lost from both types of irides in a complex fashion. However, even after 120 min of washing, the differences in accumulation remain nearly constant. When accumulation of the drug in the non-pigmented iris was analyzed by discontinuous sucrose density gradient, it was observed that the drug was bound mainly by the low density sucrose fractions where the synaptosomes separate. On the contrary, in the pigmented iris approximately 70% of the drug was found in the melanin-containing fraction. The homogenate from the substantia nigra accumulated 1.5 times more than that from the human brain cortex. The affinity of the drug for bovine iris melanin granules and the synthetic L-dopa melanin was 9.9 X 10(5) M-1 and 3.8 X 10(3) M-1, respectively. On the rabbit iris sphincter muscles, imipramine was evaluated for antimuscarinic effects. The apparent dissociation constants, KB values, for the antagonist in the non-pigmented and pigmented iris were 1.7 X 10(-7) M and 3.8 X 10(-6) M, respectively. The low antimuscarinic activity in the pigmented iris is attributed to the loss of the drug to the pigment. On this basis, relevancy of the drug binding by pigmented tissues to the effects of this tricyclic drug is discussed.", "PMID": 954838}
{"id": "pubmed23n0032_1381", "title": "The effect of local anaesthetics on the vasoconstrictor response of the isolated perfused artery to adrenaline and noradrenaline.", "content": "The vascular response to intraluminally and extraluminally administered catecholamines mixed in combination with local anaesthetics has been studied using the isolated perfused rabbit ear artery as the model system. Potentiation of the vascular response to extraluminal adrenaline was observed when prilocaine, lignocaine or cocaine were combined in low concentrations with the vasoconstrictor. No potentiation of the vascular response to extraluminally administered noradrenaline could be demonstrated in the presence of prilocaine or lignocaine. Further, no potentiation to either catecholamine was found if the artery had been denervated or the drugs were applied intraluminally. Possible mechanisms for the potentiation of extraluminally applied adrenaline are discussed.", "contents": "The effect of local anaesthetics on the vasoconstrictor response of the isolated perfused artery to adrenaline and noradrenaline. The vascular response to intraluminally and extraluminally administered catecholamines mixed in combination with local anaesthetics has been studied using the isolated perfused rabbit ear artery as the model system. Potentiation of the vascular response to extraluminal adrenaline was observed when prilocaine, lignocaine or cocaine were combined in low concentrations with the vasoconstrictor. No potentiation of the vascular response to extraluminally administered noradrenaline could be demonstrated in the presence of prilocaine or lignocaine. Further, no potentiation to either catecholamine was found if the artery had been denervated or the drugs were applied intraluminally. Possible mechanisms for the potentiation of extraluminally applied adrenaline are discussed.", "PMID": 954839}
{"id": "pubmed23n0032_1382", "title": "The interaction of delta9-tetrahydrocannabinol with cholinomimetic drugs in an agonist-antagonist paradigm.", "content": "In rabbits, i.v. delta9-tetrahydrocannabinol (delta9-THC) at 0.5 mg/kg was challenged with i.v. arecoline (0.025 mg/kg), nicotine (0.02 mg/kg) or physostigmine (PHYSO, 0.05 mg/kg), and observations were made on quantified EEG and behavior. The cholinomimetics effectively reversed the cortical and hippocampal EEG alterations induced by delta9-THC. Arecoline and PHYSO temporarily reversed the behavioral-sedative effects of delta9-THC whereas the combination of delta9-THC with nicotine produced behavioral collapse preceded by behavioral disturbance.", "contents": "The interaction of delta9-tetrahydrocannabinol with cholinomimetic drugs in an agonist-antagonist paradigm. In rabbits, i.v. delta9-tetrahydrocannabinol (delta9-THC) at 0.5 mg/kg was challenged with i.v. arecoline (0.025 mg/kg), nicotine (0.02 mg/kg) or physostigmine (PHYSO, 0.05 mg/kg), and observations were made on quantified EEG and behavior. The cholinomimetics effectively reversed the cortical and hippocampal EEG alterations induced by delta9-THC. Arecoline and PHYSO temporarily reversed the behavioral-sedative effects of delta9-THC whereas the combination of delta9-THC with nicotine produced behavioral collapse preceded by behavioral disturbance.", "PMID": 954840}
{"id": "pubmed23n0032_1383", "title": "Depression of rat cerebral cortical neurones by H1 and H2 histamine receptor agonists.", "content": "Histamine (H) and H1 agonists 2-pyridylethylamine (PEA) and 2-methylhistamine (2-MH) produced a greater depression of the corticospinal and unidentified rat cerebral cortical neurones than did 4-methylhistamine (4-MH), an H2 agonist. Mepyramine antagonized the effects of 2-MH, PEA and H, and partially antagonized the depression induced by 4-MH. Metiamide and cimetidine, H2 antagonists, blocked 4-MH and H but not 2-MH- and PEA-induced depression. These results indicate that H-induced depression of cortical neurones involves activation of H1 and H2 receptors.", "contents": "Depression of rat cerebral cortical neurones by H1 and H2 histamine receptor agonists. Histamine (H) and H1 agonists 2-pyridylethylamine (PEA) and 2-methylhistamine (2-MH) produced a greater depression of the corticospinal and unidentified rat cerebral cortical neurones than did 4-methylhistamine (4-MH), an H2 agonist. Mepyramine antagonized the effects of 2-MH, PEA and H, and partially antagonized the depression induced by 4-MH. Metiamide and cimetidine, H2 antagonists, blocked 4-MH and H but not 2-MH- and PEA-induced depression. These results indicate that H-induced depression of cortical neurones involves activation of H1 and H2 receptors.", "PMID": 954841}
{"id": "pubmed23n0032_1384", "title": "Regional rat brain levels of 3,4-dihydroxyphenylacetic acid and homovanillic acid: concurrent fluorometric measurement and influence of drugs.", "content": "A concurrent semi-automatic fluorometric assay technique for 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), is described. The method is based on a rapid manually performed isolation of DOPAC and HVA on small columns of Sephadex G-10 followed by an automated fluorometric detection with a continuous flow system. DOPAC and HVA were measured in the corpus striatum, nucleus accumbens and olfactory tubercle of the rat, under normal conditions and after treatment with amphetamine, apomorphine, clozapine, haloperidol, morphine, oxotremorine, pargyline, probenecid, sulpiride and thioridazine. Clozapine, morphine, sulpiride and oxotremorine induced the most pronounced rise of DA metabolites in the nucleus accumbens. Probenecid produced a DOPAC accumulation in the nucleus accumbens. Striking differences were observed between the DOPAC/HVA ratios in the different structures of control animals. The concurrent assay enables a rapid screening of the action of drugs in regional DA metabolism.", "contents": "Regional rat brain levels of 3,4-dihydroxyphenylacetic acid and homovanillic acid: concurrent fluorometric measurement and influence of drugs. A concurrent semi-automatic fluorometric assay technique for 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), is described. The method is based on a rapid manually performed isolation of DOPAC and HVA on small columns of Sephadex G-10 followed by an automated fluorometric detection with a continuous flow system. DOPAC and HVA were measured in the corpus striatum, nucleus accumbens and olfactory tubercle of the rat, under normal conditions and after treatment with amphetamine, apomorphine, clozapine, haloperidol, morphine, oxotremorine, pargyline, probenecid, sulpiride and thioridazine. Clozapine, morphine, sulpiride and oxotremorine induced the most pronounced rise of DA metabolites in the nucleus accumbens. Probenecid produced a DOPAC accumulation in the nucleus accumbens. Striking differences were observed between the DOPAC/HVA ratios in the different structures of control animals. The concurrent assay enables a rapid screening of the action of drugs in regional DA metabolism.", "PMID": 954842}
{"id": "pubmed23n0032_1385", "title": "Action of clonidine on the baroreceptor pathway and medullary sites mediating vagal bradycardia.", "content": "In anaesthetized dogs, clonidine (10 mug/kg i.v.) increased the spontaneous firing of the carotid sinus nerve and decreased blood pressure and heart rate. After transection of the spinal cord, clonidine decreased heart rate and this bradycardia was abolished by selective baroreceptor denervation. Clonidine (1 mug/kg) injected into the vertebral artery of anaesthetized dogs, pretreated with a beta-adrenoceptor blocking agent (S 2395: 50 mug/kg i.v.) potentiated the bradycardia induced by stimulation of the carotid sinus nerve but did not change the hypotension and bradycardia produced by stimulation of the nucleus tractus solitarius or of the nucleus ambiguus. In anaesthetized cats with bilateral destruction of nuclei tractus solitarii, clonidine (10 mug/kg i.v.) decreased blood pressure and heart rate. Clonidine (2 mug/kg), injected into the vertebral artery of anaesthetized dogs pretreated with a beta-adrenergic blocking agent (S 2395: 50 mug/kg i.v.) or guanethidine, induced a bradycardia but the discharges of the carotid sinus nerve were not increased. Selective baroreceptor denervation abolished this bradycardia. In conclusion, these experiments provide direct evidence that the central facilitory effect of clonidine on baroreceptor impulses play a role in the bradycardic effect of the drug. This facilitation is likely localized in the nucleus tractus solitarius at the first synapse of baroreceptor fibres. The vagally mediated bradycardia can be explained by an increase in baroreceptor discharges and by the central facilitation of baroreceptor impulses. The site of the hypotensive effect of clonidine did not seem to be localized in the nucleus tractus solitarius.", "contents": "Action of clonidine on the baroreceptor pathway and medullary sites mediating vagal bradycardia. In anaesthetized dogs, clonidine (10 mug/kg i.v.) increased the spontaneous firing of the carotid sinus nerve and decreased blood pressure and heart rate. After transection of the spinal cord, clonidine decreased heart rate and this bradycardia was abolished by selective baroreceptor denervation. Clonidine (1 mug/kg) injected into the vertebral artery of anaesthetized dogs, pretreated with a beta-adrenoceptor blocking agent (S 2395: 50 mug/kg i.v.) potentiated the bradycardia induced by stimulation of the carotid sinus nerve but did not change the hypotension and bradycardia produced by stimulation of the nucleus tractus solitarius or of the nucleus ambiguus. In anaesthetized cats with bilateral destruction of nuclei tractus solitarii, clonidine (10 mug/kg i.v.) decreased blood pressure and heart rate. Clonidine (2 mug/kg), injected into the vertebral artery of anaesthetized dogs pretreated with a beta-adrenergic blocking agent (S 2395: 50 mug/kg i.v.) or guanethidine, induced a bradycardia but the discharges of the carotid sinus nerve were not increased. Selective baroreceptor denervation abolished this bradycardia. In conclusion, these experiments provide direct evidence that the central facilitory effect of clonidine on baroreceptor impulses play a role in the bradycardic effect of the drug. This facilitation is likely localized in the nucleus tractus solitarius at the first synapse of baroreceptor fibres. The vagally mediated bradycardia can be explained by an increase in baroreceptor discharges and by the central facilitation of baroreceptor impulses. The site of the hypotensive effect of clonidine did not seem to be localized in the nucleus tractus solitarius.", "PMID": 954843}
{"id": "pubmed23n0032_1386", "title": "Discriminative pentobarbital stimulus in rats immediately after intravenous administration.", "content": "Rats with chronically implanted venous cannulas were trained to make differential active or passive shock-avoidance responses in a 2-compartment box at 15 sec after i.v. adminstration of pentobarbital (5 mg/kg) or saline. The discriminative pentobarbital stimulus persisted for several min after adminstration. The pentobarbital response also was elicited in tests at 15 sec after i.v. injection of chlordiazepoxide (2.5 and 5 mg/kg) or alcohol (500 mg/kg). Subsequent training at 10 min after i.p. injection of pentobarbital (10 mg/kg), followed by tests after i.p. administration of other drugs, indicated that the discriminative drug stimulus is qualitatively similar with this different route and time interval. A general preference for the passive over the active response developed only in the second stage of training, during i.p. injections.", "contents": "Discriminative pentobarbital stimulus in rats immediately after intravenous administration. Rats with chronically implanted venous cannulas were trained to make differential active or passive shock-avoidance responses in a 2-compartment box at 15 sec after i.v. adminstration of pentobarbital (5 mg/kg) or saline. The discriminative pentobarbital stimulus persisted for several min after adminstration. The pentobarbital response also was elicited in tests at 15 sec after i.v. injection of chlordiazepoxide (2.5 and 5 mg/kg) or alcohol (500 mg/kg). Subsequent training at 10 min after i.p. injection of pentobarbital (10 mg/kg), followed by tests after i.p. administration of other drugs, indicated that the discriminative drug stimulus is qualitatively similar with this different route and time interval. A general preference for the passive over the active response developed only in the second stage of training, during i.p. injections.", "PMID": 954844}
{"id": "pubmed23n0032_1387", "title": "Studies relating morphine hypothermia with serotonin reuptake in the rat hypothalamus.", "content": "Experiments were conducted to determine if the acute hypothermia elicited by morphine in the rat resulted from morphine-induced inhibition of serotonin reuptake into hypothalamic nerve-endings. The acute hypothermia elicited by morphine (30 mg/kg, s.c.) was reversed by pretreatment with naloxone (10 mg/kg, s.c.), a narcotic antagonist, which alone did not alter body temperature. In vitro uptake of serotonin into synaptosomes prepared from rat hypothalamus was inhibited 53% by the addition of morphine (7 X 10(-4) M) and 53.9% by naloxone (1 X 10(-3) M). Simultaneous addition of both drugs in these same concentrations further inhibited synaptosomal serotonin uptake 77.8%. Uptake of serotonin uptake into synaptosomes isolated from rats injected with morphine (30 mg/kg, s.c.) was not altered as compared to controls. These data suggest that the acute hypothermic acition in morphine in the rat is not related to an inhibition of the serotonin reuptake mechanism in hypothalamic nerve-endings.", "contents": "Studies relating morphine hypothermia with serotonin reuptake in the rat hypothalamus. Experiments were conducted to determine if the acute hypothermia elicited by morphine in the rat resulted from morphine-induced inhibition of serotonin reuptake into hypothalamic nerve-endings. The acute hypothermia elicited by morphine (30 mg/kg, s.c.) was reversed by pretreatment with naloxone (10 mg/kg, s.c.), a narcotic antagonist, which alone did not alter body temperature. In vitro uptake of serotonin into synaptosomes prepared from rat hypothalamus was inhibited 53% by the addition of morphine (7 X 10(-4) M) and 53.9% by naloxone (1 X 10(-3) M). Simultaneous addition of both drugs in these same concentrations further inhibited synaptosomal serotonin uptake 77.8%. Uptake of serotonin uptake into synaptosomes isolated from rats injected with morphine (30 mg/kg, s.c.) was not altered as compared to controls. These data suggest that the acute hypothermic acition in morphine in the rat is not related to an inhibition of the serotonin reuptake mechanism in hypothalamic nerve-endings.", "PMID": 954845}
{"id": "pubmed23n0032_1388", "title": "Effects of zinc chloride on gastric secretion and ulcer formation in pylorus-occluded rats.", "content": "The effects of 10-day pretreatment with i.p. injections of zinc chloride, 16 mg/kg, on gastric secretion and on gastric ulceration induced by stress or by acid accumulation were examined in pylorus-occluded rats. Zinc chloride pretreatment significantly reduced the volume of gastric secretion and the total acid output as well as the incidence of gastric ulcers induced either by stress or by acid accumulation. The findings support the idea zinc compounds may be useful in the treatment of gastric ulcers.", "contents": "Effects of zinc chloride on gastric secretion and ulcer formation in pylorus-occluded rats. The effects of 10-day pretreatment with i.p. injections of zinc chloride, 16 mg/kg, on gastric secretion and on gastric ulceration induced by stress or by acid accumulation were examined in pylorus-occluded rats. Zinc chloride pretreatment significantly reduced the volume of gastric secretion and the total acid output as well as the incidence of gastric ulcers induced either by stress or by acid accumulation. The findings support the idea zinc compounds may be useful in the treatment of gastric ulcers.", "PMID": 954846}
{"id": "pubmed23n0032_1389", "title": "The effect of tricyclic antidepressants and neuroleptics on the peripheral and central action of norepinephrine in reserpine-treated mice.", "content": "The effect of exogenous norepinephrine on the ptosis induced by reserpine and its modification by tricyclic antidepressants and neuroleptics were studied in reserpine-pretreated mice. S.c injection of norepinephrine (0.3-5 mg/kg) reversed dose-dependently the ptosis induced by reserpine. The maximal effect was obtained 15 min after norepinephrine administration. Tricyclic antidepressants (2.5 and 5 mg/kg i.p.) potentiated the effect of norepinephrine. In contrast neuroleptics (1 and 5 mg/kg i.p.) antagonized it. Intracerebral injection of norepinephrine (5-20 mug) also reversed dose-dependently the ptosis induced by reserpine, and the maximal effect was obtained within 5 min. Tricyclic antidepressants potentiated the effect of norepinephrine, but neuroleptics antagonized it. Among tricyclic antidepressants, the potentiating action of secondary amines was stronger than that of tertiary amines. Chlorpromazine blocked the action of norepinephrine more strongly than did the same dose of haloperidol.", "contents": "The effect of tricyclic antidepressants and neuroleptics on the peripheral and central action of norepinephrine in reserpine-treated mice. The effect of exogenous norepinephrine on the ptosis induced by reserpine and its modification by tricyclic antidepressants and neuroleptics were studied in reserpine-pretreated mice. S.c injection of norepinephrine (0.3-5 mg/kg) reversed dose-dependently the ptosis induced by reserpine. The maximal effect was obtained 15 min after norepinephrine administration. Tricyclic antidepressants (2.5 and 5 mg/kg i.p.) potentiated the effect of norepinephrine. In contrast neuroleptics (1 and 5 mg/kg i.p.) antagonized it. Intracerebral injection of norepinephrine (5-20 mug) also reversed dose-dependently the ptosis induced by reserpine, and the maximal effect was obtained within 5 min. Tricyclic antidepressants potentiated the effect of norepinephrine, but neuroleptics antagonized it. Among tricyclic antidepressants, the potentiating action of secondary amines was stronger than that of tertiary amines. Chlorpromazine blocked the action of norepinephrine more strongly than did the same dose of haloperidol.", "PMID": 954847}
{"id": "pubmed23n0032_1390", "title": "Effect of antihypertensive therapy on lysine incorporation into vascular protein of the spontaneously hypertensive rat.", "content": "Young spontaneously hypertensive rats (SHR) were either treated with hydralazine or hexamethonium or splanchnicotomized, so that the development of hypertension was effectively arrested for two weeks. The rate of incorporation of 3H-lysine into non-collagenous proteins in vivo of the heart, aorta and mesenteric arteries was determined in the treated SHR, as well as control SHR and normal Wistar/Kyoto (WK) rats. The lysine incorporation into the non-collagenous protein of mesenteric arteries was increased in 8-week-old SHR as compared with WK rats. Teh elevated lysine incorporation in the SHR was abolished by treatment with hexamethonium or by splanchnicotomy, but was not affected by treatment with hydralazine. It is suggested that sympathetic innervation is important fot the increased synthesis of vascular non-collagenous protein during the early hypertensive phase in the SHR.", "contents": "Effect of antihypertensive therapy on lysine incorporation into vascular protein of the spontaneously hypertensive rat. Young spontaneously hypertensive rats (SHR) were either treated with hydralazine or hexamethonium or splanchnicotomized, so that the development of hypertension was effectively arrested for two weeks. The rate of incorporation of 3H-lysine into non-collagenous proteins in vivo of the heart, aorta and mesenteric arteries was determined in the treated SHR, as well as control SHR and normal Wistar/Kyoto (WK) rats. The lysine incorporation into the non-collagenous protein of mesenteric arteries was increased in 8-week-old SHR as compared with WK rats. Teh elevated lysine incorporation in the SHR was abolished by treatment with hexamethonium or by splanchnicotomy, but was not affected by treatment with hydralazine. It is suggested that sympathetic innervation is important fot the increased synthesis of vascular non-collagenous protein during the early hypertensive phase in the SHR.", "PMID": 954848}
{"id": "pubmed23n0032_1391", "title": "Loss of an adrenergic effect in swiss mice.", "content": "The effect of adrenaline on the amount of blood eosinophils (eosinophilia) which had been found in Swiss mice in earlier experiments was lost after a few generations. The hyperglycemic response to adrenaline had not been changed. Sensitivities to noradrenaline and adrenaline were as found earlier, but the sensitivity to isoprenaline was reduced. The reduction of beta-adrenergic responsiveness suggests variations in biochemical parameters of mice or the appearance of mice genetically less responsive to beta-adrenergic drugs in only a few generations.", "contents": "Loss of an adrenergic effect in swiss mice. The effect of adrenaline on the amount of blood eosinophils (eosinophilia) which had been found in Swiss mice in earlier experiments was lost after a few generations. The hyperglycemic response to adrenaline had not been changed. Sensitivities to noradrenaline and adrenaline were as found earlier, but the sensitivity to isoprenaline was reduced. The reduction of beta-adrenergic responsiveness suggests variations in biochemical parameters of mice or the appearance of mice genetically less responsive to beta-adrenergic drugs in only a few generations.", "PMID": 954849}
{"id": "pubmed23n0032_1392", "title": "Enkephalin: a potential modulator of noradrenaline release in rat brain.", "content": "In slices of rat occipital cortex preincubated with 3H-noradrenaline, methioine-enkephalin diminished the overflow of tritium evoked by electrical field stimulation or by 20 mM potassium. The effect was not modified by phentolamine, but was antagonized by naloxone. Giver alone, naloxone slightly increased the potassium-evoked overflow. It is concluded that enkephalin reduces transmitter release from cerebrocortical noradrenergic nerve endings. One-function of endogenous opiate receptor ligands may be presynaptic inhibition of central noradrenergic transmission.", "contents": "Enkephalin: a potential modulator of noradrenaline release in rat brain. In slices of rat occipital cortex preincubated with 3H-noradrenaline, methioine-enkephalin diminished the overflow of tritium evoked by electrical field stimulation or by 20 mM potassium. The effect was not modified by phentolamine, but was antagonized by naloxone. Giver alone, naloxone slightly increased the potassium-evoked overflow. It is concluded that enkephalin reduces transmitter release from cerebrocortical noradrenergic nerve endings. One-function of endogenous opiate receptor ligands may be presynaptic inhibition of central noradrenergic transmission.", "PMID": 954850}
{"id": "pubmed23n0032_1393", "title": "[Analysis of digoxin by a radio-immunologic].", "content": "A more accurate knowledge of the effects of cardiotonic heterosides can be gained using radiocompetition. The present investigation aimed at: -comparing two commercial kits, the usual quality criteria being applied; -estimating cross-reaction between the immune systems and the endogenous as well as exogenous structural analogues; -establishing the therapeutical or toxic levels in man.", "contents": "[Analysis of digoxin by a radio-immunologic]. A more accurate knowledge of the effects of cardiotonic heterosides can be gained using radiocompetition. The present investigation aimed at: -comparing two commercial kits, the usual quality criteria being applied; -estimating cross-reaction between the immune systems and the endogenous as well as exogenous structural analogues; -establishing the therapeutical or toxic levels in man.", "PMID": 954851}
{"id": "pubmed23n0032_1394", "title": "[Determination of acetylator phenotype of isoniazid by superimposed sinusodial tension polarography].", "content": "Alternative current polarography has been applied to specific determination of INH and acetyl-isoniazide in serum and urine. Determinations can be achieved directly on diluted urines. The method allows measure of pharmacocinetic parameters and determination of acetylation phenotype following I.V. administration of the drug.", "contents": "[Determination of acetylator phenotype of isoniazid by superimposed sinusodial tension polarography]. Alternative current polarography has been applied to specific determination of INH and acetyl-isoniazide in serum and urine. Determinations can be achieved directly on diluted urines. The method allows measure of pharmacocinetic parameters and determination of acetylation phenotype following I.V. administration of the drug.", "PMID": 954852}
{"id": "pubmed23n0032_1395", "title": "[Evoluation of biologic balance as a function of radiologic status in a population living in a endemic fluoride zone of southern Algeria].", "content": "Chronic fluoride intoxication in subjects living in an endemic South Algerian zone: El Oued (drinking water fluor: 3 to 5 mg/l), provokes some blood and urinary levels modifications, when compared to normals living in Algiers (drinking water fluor: 0,6 mg/l). These modifications are the more often present in stade 0 (normal radiologic aspect) and do not increase with radiological evolution (stades I, II, III). Fluoremy and fluorury increase. Phosphocalcic metabolism is altered. Tubular reabsorption coefficient, particularly, decreases strongly. Using renal functional exploration, a pretty soon tubular failure is founded, which preceeds glomerular failure. Blood levels of certain products and enzymes are studied.", "contents": "[Evoluation of biologic balance as a function of radiologic status in a population living in a endemic fluoride zone of southern Algeria]. Chronic fluoride intoxication in subjects living in an endemic South Algerian zone: El Oued (drinking water fluor: 3 to 5 mg/l), provokes some blood and urinary levels modifications, when compared to normals living in Algiers (drinking water fluor: 0,6 mg/l). These modifications are the more often present in stade 0 (normal radiologic aspect) and do not increase with radiological evolution (stades I, II, III). Fluoremy and fluorury increase. Phosphocalcic metabolism is altered. Tubular reabsorption coefficient, particularly, decreases strongly. Using renal functional exploration, a pretty soon tubular failure is founded, which preceeds glomerular failure. Blood levels of certain products and enzymes are studied.", "PMID": 954855}
{"id": "pubmed23n0032_1396", "title": "[Irritant power of the principle chlorinated aliphatic solvents on the skin and ocular mucosa of the rabbit].", "content": "A study was carried out in the rabbit in order to compare the cutaneous and ocular mucosa irritant powers of the principal aliphatic chloride solvents and of pure hexachlorobutadiene. Using Draize's method, that of the official technique for the assessment of cosmetic products and the interpretation of Kay and Calandra, a study was made of the effects of a single application or instillation of the common aliphatic chloride solvents. Macroscopic observations were followed by histological examination of the lesions and their course. With the exception of trichloro trifluoro-1,2,3-ethane, these substances are primary irritants but their effects on rabbit skin and ocular mucosa differ.", "contents": "[Irritant power of the principle chlorinated aliphatic solvents on the skin and ocular mucosa of the rabbit]. A study was carried out in the rabbit in order to compare the cutaneous and ocular mucosa irritant powers of the principal aliphatic chloride solvents and of pure hexachlorobutadiene. Using Draize's method, that of the official technique for the assessment of cosmetic products and the interpretation of Kay and Calandra, a study was made of the effects of a single application or instillation of the common aliphatic chloride solvents. Macroscopic observations were followed by histological examination of the lesions and their course. With the exception of trichloro trifluoro-1,2,3-ethane, these substances are primary irritants but their effects on rabbit skin and ocular mucosa differ.", "PMID": 954854}
{"id": "pubmed23n0032_1397", "title": "[Poisoning by anorexigenics. Information from the data bank of the Anti-Poison Center of Marseille (1973)].", "content": "Using the data bank developed by the Computer Service of the Lyon Hospitals, the Anti-Poisons Centre of Marseille presents an initial study of intoxications by appetite suppressants. The work was complemented by a simultaneous study using conventional manual techniques. An epidemiologic review of 1973 and an approximate assessment of toxic doses in the adult and child were possible. They should be of particular use in answering telephone enquiries at anti-poison centres.", "contents": "[Poisoning by anorexigenics. Information from the data bank of the Anti-Poison Center of Marseille (1973)]. Using the data bank developed by the Computer Service of the Lyon Hospitals, the Anti-Poisons Centre of Marseille presents an initial study of intoxications by appetite suppressants. The work was complemented by a simultaneous study using conventional manual techniques. An epidemiologic review of 1973 and an approximate assessment of toxic doses in the adult and child were possible. They should be of particular use in answering telephone enquiries at anti-poison centres.", "PMID": 954856}
{"id": "pubmed23n0032_1398", "title": "[Role of the laboratory in the diagnosis and treatment of lead poisoning].", "content": "A case of therapeutic lead poisoning in a six-week old child is reported, this aetiology being very common in paediatrics. The role of the laboratory in treatment is described, the latter having involved the addition of disodium calcitetracemate to the peritoneal dialysis fluid.", "contents": "[Role of the laboratory in the diagnosis and treatment of lead poisoning]. A case of therapeutic lead poisoning in a six-week old child is reported, this aetiology being very common in paediatrics. The role of the laboratory in treatment is described, the latter having involved the addition of disodium calcitetracemate to the peritoneal dialysis fluid.", "PMID": 954853}
{"id": "pubmed23n0032_1399", "title": "On the mechanism of erythropoietin-induced differentiation. XIV. The apparent effect of etiocholanolone on initiation of erythropoiesis.", "content": "Etiocholanolone, when tested in normal mice or in mice that have been out of an hypoxic atmosphere for only a few days, stimulates erythropoiesis, but appears to have no effect on erythropoiesis when tested in plethoric mice that have very low residual red cell formation. The steroid, similarly, stimulates hemoglobin synthesis by marrow cells in culture if they are from normal mice, but has no effect on cells from transfused mice. Out data suggest that 5 beta-H androstane and pregnane derivatives do not have a primary action on the induction of erythropoiesis, but act on cells that are already differentiated.", "contents": "On the mechanism of erythropoietin-induced differentiation. XIV. The apparent effect of etiocholanolone on initiation of erythropoiesis. Etiocholanolone, when tested in normal mice or in mice that have been out of an hypoxic atmosphere for only a few days, stimulates erythropoiesis, but appears to have no effect on erythropoiesis when tested in plethoric mice that have very low residual red cell formation. The steroid, similarly, stimulates hemoglobin synthesis by marrow cells in culture if they are from normal mice, but has no effect on cells from transfused mice. Out data suggest that 5 beta-H androstane and pregnane derivatives do not have a primary action on the induction of erythropoiesis, but act on cells that are already differentiated.", "PMID": 954881}
{"id": "pubmed23n0032_1400", "title": "Nonbursal origin of humoral immunity: immune capacity and cytomorphological changes in chickens bursectomized as 52- to 64-hours-old embryos.", "content": "Chickens sham-bursectomized as 52- to 64-hours-old embryos (SBx), chickens bursectomized at hatching (NeoBx) and chickens from which the bursal primordium was removed at 52-64 h of embryonation (EBx) were immunized with guinea pig red blood cells when 21 days old. Following the second injection of antigen, EBx chickens were found to elaborate ME-sensitive and ME-resistant hemagglutinins, to produce direct and indirect plaque-forming cells, and to contain lymphocytes bearing a specific bursa (Bu) antigen. These immune properties were more often expressed in sham-bursectomized and neonatally bursectomized chickens. Cytomorphological changes in the spleen, bone marrow and cecal tonsil of EBx chickens were typical for birds lacking the bursa. The number of lymphocytes and plasma cells in the bone marrow and liver of SBx, NeoBx and EBx chickens was very low. These results suggest that a very early embryonic bursectomy of chickens does not abolish humoral immunity completely and does not prevent the differentiation of Bu antigen-bearing lymphocytes.", "contents": "Nonbursal origin of humoral immunity: immune capacity and cytomorphological changes in chickens bursectomized as 52- to 64-hours-old embryos. Chickens sham-bursectomized as 52- to 64-hours-old embryos (SBx), chickens bursectomized at hatching (NeoBx) and chickens from which the bursal primordium was removed at 52-64 h of embryonation (EBx) were immunized with guinea pig red blood cells when 21 days old. Following the second injection of antigen, EBx chickens were found to elaborate ME-sensitive and ME-resistant hemagglutinins, to produce direct and indirect plaque-forming cells, and to contain lymphocytes bearing a specific bursa (Bu) antigen. These immune properties were more often expressed in sham-bursectomized and neonatally bursectomized chickens. Cytomorphological changes in the spleen, bone marrow and cecal tonsil of EBx chickens were typical for birds lacking the bursa. The number of lymphocytes and plasma cells in the bone marrow and liver of SBx, NeoBx and EBx chickens was very low. These results suggest that a very early embryonic bursectomy of chickens does not abolish humoral immunity completely and does not prevent the differentiation of Bu antigen-bearing lymphocytes.", "PMID": 954882}
{"id": "pubmed23n0032_1401", "title": "Galactolipid fatty acids in brain of pyridoxine-deficient young rats.", "content": "The non-hydroxy fatty acids of the galactolipid fraction of brain lipids of pyridoxine-deficient rats have been analyzed. The results suggest an impairment in the pathway for the synthesis of very long chain fatty acids in the brain of pyridoxine-deficient rats.", "contents": "Galactolipid fatty acids in brain of pyridoxine-deficient young rats. The non-hydroxy fatty acids of the galactolipid fraction of brain lipids of pyridoxine-deficient rats have been analyzed. The results suggest an impairment in the pathway for the synthesis of very long chain fatty acids in the brain of pyridoxine-deficient rats.", "PMID": 954883}
{"id": "pubmed23n0032_1402", "title": "[Quantitative ultrastructural study of the perikaryon of the Purkinje cell and of the adjacent area in normal and hypothyroid rats aged 21 days].", "content": "The effects of neonatal hypothyroidism on synaptic organization, glial surrounding and cytoplasmic structures of the Purkinje cell perikaryon of the 21 days old rat were studied by electron microscopy. Hypothyroidism decreases the size of the perikaryon but does not change the nucleo-cytoplasmic ratio of the cell. At 21 days the axonal endings that make synapse on the Purkinje cell perikaryon, already show the adult morphological features and hypothyroidism does not change these. The general synaptic density on the Purkinje perikaryon is not significantly altered, but the cells of hypothyroid animals still recieve 1/3 of their axo-somatic synapses on somatic spines, whereas euthyroid animals have practically no synapses on somatic spines. Hypothyroidism leads the disappearance of the synchronism which normally exists between the translocation or resorption of the Purkinje cell somatic spines and the establishment of the basket cell synapses. The basket cell axons of the normal animals form synapses only on the smooth surfaces of the Purkinje cell perikaryon while they also establish it on the somatic spines of the hypothyroid animals. The density of the axon terminals of basket cells is decreased, while that of the endings of Purkinje cell axon collaterals and of climbing fibers are increased. Hypothyroidism produces an increase in the size of the glial sheath around the Purkinje perikaryon. It does not alter the proportion of the cytoplasmic area occupied by mitochondria, but in thyroid deficiency there is an increase in the numbers of mitochondria which are reduced in average size. The Golgi apparatus is only slightly affected. Thyroid defiency does not alter the density of the cytoplasmic agreggates of free ribosomes but there is a decrease in density of the ergastoplasm and a disorganization of the Nissl bodies.", "contents": "[Quantitative ultrastructural study of the perikaryon of the Purkinje cell and of the adjacent area in normal and hypothyroid rats aged 21 days]. The effects of neonatal hypothyroidism on synaptic organization, glial surrounding and cytoplasmic structures of the Purkinje cell perikaryon of the 21 days old rat were studied by electron microscopy. Hypothyroidism decreases the size of the perikaryon but does not change the nucleo-cytoplasmic ratio of the cell. At 21 days the axonal endings that make synapse on the Purkinje cell perikaryon, already show the adult morphological features and hypothyroidism does not change these. The general synaptic density on the Purkinje perikaryon is not significantly altered, but the cells of hypothyroid animals still recieve 1/3 of their axo-somatic synapses on somatic spines, whereas euthyroid animals have practically no synapses on somatic spines. Hypothyroidism leads the disappearance of the synchronism which normally exists between the translocation or resorption of the Purkinje cell somatic spines and the establishment of the basket cell synapses. The basket cell axons of the normal animals form synapses only on the smooth surfaces of the Purkinje cell perikaryon while they also establish it on the somatic spines of the hypothyroid animals. The density of the axon terminals of basket cells is decreased, while that of the endings of Purkinje cell axon collaterals and of climbing fibers are increased. Hypothyroidism produces an increase in the size of the glial sheath around the Purkinje perikaryon. It does not alter the proportion of the cytoplasmic area occupied by mitochondria, but in thyroid deficiency there is an increase in the numbers of mitochondria which are reduced in average size. The Golgi apparatus is only slightly affected. Thyroid defiency does not alter the density of the cytoplasmic agreggates of free ribosomes but there is a decrease in density of the ergastoplasm and a disorganization of the Nissl bodies.", "PMID": 954884}
{"id": "pubmed23n0032_1403", "title": "Direct neural connection from the medial preoptic area to the hypothalamic arcuate nucleus of the rat.", "content": "Direct neural connections from the medial preoptic area (MPOA) to the arcuate nucleus were studied on light and electron microscope level by a multistep experimental procedure. The hypothalamic deafferentation technique of Hal\u00e1sz and Pupp (1965) was used in itself or was combined with electrolytic lesions. In order to eliminate all fibers of more rostral source traversing the medial preoptic area an extended rostral preoptic deafferentation was made, and a survival time of at least 3 weeks was chosen to allow for the complete disappearance of the degenerated fragments from the arcuate nucleus. In the main experimental group the medial preoptic area was destroyed by an electrolytic lesion 3 or more weeks following such rostral preoptic deafferentations. In these animals degenerated fibers and terminals certainly of preoptic origin were found distributed bilaterally, with a predominance on the side of the lesion. - These data suggest that axons originating from medial preoptic neurons terminate in the arcuate nucleus, thus constituting a preoptico-tuberal pathway.", "contents": "Direct neural connection from the medial preoptic area to the hypothalamic arcuate nucleus of the rat. Direct neural connections from the medial preoptic area (MPOA) to the arcuate nucleus were studied on light and electron microscope level by a multistep experimental procedure. The hypothalamic deafferentation technique of Hal\u00e1sz and Pupp (1965) was used in itself or was combined with electrolytic lesions. In order to eliminate all fibers of more rostral source traversing the medial preoptic area an extended rostral preoptic deafferentation was made, and a survival time of at least 3 weeks was chosen to allow for the complete disappearance of the degenerated fragments from the arcuate nucleus. In the main experimental group the medial preoptic area was destroyed by an electrolytic lesion 3 or more weeks following such rostral preoptic deafferentations. In these animals degenerated fibers and terminals certainly of preoptic origin were found distributed bilaterally, with a predominance on the side of the lesion. - These data suggest that axons originating from medial preoptic neurons terminate in the arcuate nucleus, thus constituting a preoptico-tuberal pathway.", "PMID": 954885}
{"id": "pubmed23n0032_1404", "title": "Cat retinal ganglion cells projecting to the superior colliculus as shown by the horseradish peroxidase method.", "content": "1. Cat retinal ganglion cells projecting to the superior colliculus (SC) were detected in flat whole-mounts of Nissl-stained retinas by means of unilateral intracollicular injections of horseradish peroxidase (HRP). 2. With the purpose of drawing a comparison between HRP-labeled ganglion cells and the remaining population of unlabeled ganglion cells, the latter were classified according to their perikaryal characteristics into three groups: large, medium and small ganglion cells. Measurements of perikaryal sizes of ganglion cells distributed from the central area to the periphery of the retina showed that large cells ranged from 20 to 44 mum, medium cells from 15 to 30 mum and small cells from 8 to 17 mum. 3. Peroxidase-labeled retinal ganglion cells (HRP-cells) occurred among representatives of each of the three groups into which retinal ganglion cells had been classified. Small HRP-cells clearly constituted the majority of the HRP-cell population. When the injection site was restricted to the superior colliculus, medium HRP-cells as large as 19 mum were only occasionally observed, the 15 to 18 mum range being predominant over the medium cell population. 4. Medium HRP-cells larger than 20 mum were a common finding only when the injection site involved the pretectal nuclei and were never observed when the injection site was restricted to superior colliculus. 5. Possibility of a correspondence between the large, medium and small HRP-cells of the present study with, respectively, the physiological types Y-, X- and W- of cat retinal ganglion cells is discussed.", "contents": "Cat retinal ganglion cells projecting to the superior colliculus as shown by the horseradish peroxidase method. 1. Cat retinal ganglion cells projecting to the superior colliculus (SC) were detected in flat whole-mounts of Nissl-stained retinas by means of unilateral intracollicular injections of horseradish peroxidase (HRP). 2. With the purpose of drawing a comparison between HRP-labeled ganglion cells and the remaining population of unlabeled ganglion cells, the latter were classified according to their perikaryal characteristics into three groups: large, medium and small ganglion cells. Measurements of perikaryal sizes of ganglion cells distributed from the central area to the periphery of the retina showed that large cells ranged from 20 to 44 mum, medium cells from 15 to 30 mum and small cells from 8 to 17 mum. 3. Peroxidase-labeled retinal ganglion cells (HRP-cells) occurred among representatives of each of the three groups into which retinal ganglion cells had been classified. Small HRP-cells clearly constituted the majority of the HRP-cell population. When the injection site was restricted to the superior colliculus, medium HRP-cells as large as 19 mum were only occasionally observed, the 15 to 18 mum range being predominant over the medium cell population. 4. Medium HRP-cells larger than 20 mum were a common finding only when the injection site involved the pretectal nuclei and were never observed when the injection site was restricted to superior colliculus. 5. Possibility of a correspondence between the large, medium and small HRP-cells of the present study with, respectively, the physiological types Y-, X- and W- of cat retinal ganglion cells is discussed.", "PMID": 954886}
{"id": "pubmed23n0032_1405", "title": "Transcerebellar inhibitory interaction between the bilateral vestibular nuclei and its modulation by cerebellocortical activity.", "content": "In decerebrate, unanesthetized cats, the brain stem was longitudinally cut at the midline from its dorsal to ventral surface with the cerebellum kept intact, eliminating neural interactions between the bilateral vestibular nuclei through the brain stem. Extracellular spike potentials of vestibular type I neurons identified by horizontal rotation were distinctly inhibited by contralateral vestibular nerve stimulation. This crossed inhibition was abolished by removal of the medial part of the cerebellum, indicating that the inhibition was mediated through the cerebellum. Neither aspiration of the flocculus on the recording side nor intravenous administration of picrotoxin eliminated transcerebellar crossed inhibition, suggesting that it is mediated through the cerebellar nuclei. When the fastigial, interposite and dentate nuclei were stimulated, inhibition of vestibular type I neurons was produced only from the contralateral fastigal nucleus. Cerebellocortical stimulation which inhibited fastigial type I neurons suppressed transcerebellar crossed inhibition. Effective sites for suppression of transcerebellar crossed inhibition were localized to lobules VI and VIIa in the vermal cortex on the side of labyrinthine stimulation. Intracellular recordings were made from type I neurons in the medial vestibular nucleus. Stimulation of the contralateral vestibular nerve and the contralateral fastigial nucleus produced IPSPs in these neurons with the shortest latency of 3.8 msec and 1.8 msec, respectively. The difference between these two latency values approximates the shortest latency of spike initiation of fastigial type I neurons in response to vestibular nerve stimulation. It is postulated that transcerebellar crossed inhibition is mediated through the fastigial nucleus on the side of labyrinthine stimulation.", "contents": "Transcerebellar inhibitory interaction between the bilateral vestibular nuclei and its modulation by cerebellocortical activity. In decerebrate, unanesthetized cats, the brain stem was longitudinally cut at the midline from its dorsal to ventral surface with the cerebellum kept intact, eliminating neural interactions between the bilateral vestibular nuclei through the brain stem. Extracellular spike potentials of vestibular type I neurons identified by horizontal rotation were distinctly inhibited by contralateral vestibular nerve stimulation. This crossed inhibition was abolished by removal of the medial part of the cerebellum, indicating that the inhibition was mediated through the cerebellum. Neither aspiration of the flocculus on the recording side nor intravenous administration of picrotoxin eliminated transcerebellar crossed inhibition, suggesting that it is mediated through the cerebellar nuclei. When the fastigial, interposite and dentate nuclei were stimulated, inhibition of vestibular type I neurons was produced only from the contralateral fastigal nucleus. Cerebellocortical stimulation which inhibited fastigial type I neurons suppressed transcerebellar crossed inhibition. Effective sites for suppression of transcerebellar crossed inhibition were localized to lobules VI and VIIa in the vermal cortex on the side of labyrinthine stimulation. Intracellular recordings were made from type I neurons in the medial vestibular nucleus. Stimulation of the contralateral vestibular nerve and the contralateral fastigial nucleus produced IPSPs in these neurons with the shortest latency of 3.8 msec and 1.8 msec, respectively. The difference between these two latency values approximates the shortest latency of spike initiation of fastigial type I neurons in response to vestibular nerve stimulation. It is postulated that transcerebellar crossed inhibition is mediated through the fastigial nucleus on the side of labyrinthine stimulation.", "PMID": 954888}
{"id": "pubmed23n0032_1406", "title": "Effects of tectal slits on the anomalous corticotectal fibres.", "content": "Following cortical lesion and a mid-saggital cut through the tectum in newborn albino rats, the remaining intact cortex was seen to project bilaterally to the superior colliculus with anomalous fibres running across the mid-line to the contralateral superior colliculus. In animals lesioned at day 5 postnatally and later, the anomalous fibres were mostly deflected by the scar tissue resulting from the tectal injury. In animals lesioned within the first 2 days after birth, a good proportion of the anomalous fibres coursed through the scar tissue. In all animals studied except one the pattern of terminations of the anomalous fibres was in agreement with the observations of Leong and Lund.", "contents": "Effects of tectal slits on the anomalous corticotectal fibres. Following cortical lesion and a mid-saggital cut through the tectum in newborn albino rats, the remaining intact cortex was seen to project bilaterally to the superior colliculus with anomalous fibres running across the mid-line to the contralateral superior colliculus. In animals lesioned at day 5 postnatally and later, the anomalous fibres were mostly deflected by the scar tissue resulting from the tectal injury. In animals lesioned within the first 2 days after birth, a good proportion of the anomalous fibres coursed through the scar tissue. In all animals studied except one the pattern of terminations of the anomalous fibres was in agreement with the observations of Leong and Lund.", "PMID": 954889}
{"id": "pubmed23n0032_1407", "title": "The integrative role of the intralaminar system of the thalamus in visual orientation and perception in the cat.", "content": "Electrical stimulation with repetitive pulses (6/sec) was applied to the internal medullary lamina and produced tonic contraversive turning movements of eyes and head which were associated with recruiting responses in the visual cortex. Optical gratings of a spatial frequency of 0.5 c/deg which alternated with diffuse illumination of equal intensity were then presented to the animal during conjugated adversive eye movements induced by intralaminar stimulation with continuous sine-wave alternating currents of 5000 c.p.s. The head of the animal was fixed except for rotation in the horizontal plane. Prior to intralaminar stimulation it was turned in the direction opposite to the induced eye movements so that the animal always viewed the central area of the screen. The potential recorded in the visual cortex in response to the gratings consisted of an initial positive-negative potential of the order of 20 muV followed by later deflections of irregular shape. The size of this initial potential was measured during induced gazing and in the absence of intralaminar stimulation. A significantly greater potential in response to the gratings was found during gazing. Diminishing the visual acuity of the animal resulted in a marked decrease of the initial potential and in this condition very little increase was obtained during gazing. No enhancement of the potential was seen when stimulation was applied to other thalamic structures that yielded no visual orientation. It is concluded that perception of contrast is improved during visual orientation and that the effect is due to a concomitant activation of unspecific intralaminar afferents to the visual cortex.", "contents": "The integrative role of the intralaminar system of the thalamus in visual orientation and perception in the cat. Electrical stimulation with repetitive pulses (6/sec) was applied to the internal medullary lamina and produced tonic contraversive turning movements of eyes and head which were associated with recruiting responses in the visual cortex. Optical gratings of a spatial frequency of 0.5 c/deg which alternated with diffuse illumination of equal intensity were then presented to the animal during conjugated adversive eye movements induced by intralaminar stimulation with continuous sine-wave alternating currents of 5000 c.p.s. The head of the animal was fixed except for rotation in the horizontal plane. Prior to intralaminar stimulation it was turned in the direction opposite to the induced eye movements so that the animal always viewed the central area of the screen. The potential recorded in the visual cortex in response to the gratings consisted of an initial positive-negative potential of the order of 20 muV followed by later deflections of irregular shape. The size of this initial potential was measured during induced gazing and in the absence of intralaminar stimulation. A significantly greater potential in response to the gratings was found during gazing. Diminishing the visual acuity of the animal resulted in a marked decrease of the initial potential and in this condition very little increase was obtained during gazing. No enhancement of the potential was seen when stimulation was applied to other thalamic structures that yielded no visual orientation. It is concluded that perception of contrast is improved during visual orientation and that the effect is due to a concomitant activation of unspecific intralaminar afferents to the visual cortex.", "PMID": 954890}
{"id": "pubmed23n0032_1408", "title": "Field potentials in the olfactory bulb of the rainbow trout (Salmo gairdneri): evidence for a dendrodendritic inhibitory pathway.", "content": "Field potentials have been recorded from the olfactory bulb of the teleost fish, Salmo gairdneri. Stimulation of the olfactory nerve generates a complex wave pattern, which when recorded near the surface in the centre of the bulb consists of one positive and three negative components, P, N1, N2 and N3. The N1 has been attributed to depolarization of mitral cell dendrites, N2 to depolarization of the superficial dendrites of granules cells, and P to subsequent hyperpolarization of mitral cells. Possible origins of the N3 wave are discussed. Spikes from single units recorded in the mitral cell layer usually occur superimposed on the N1 wave, and single units recorded in the region of granule cells are usually found coincident with the trailling edge of the deep positive wave. With the exception of N3 the patterns of activity observed are very similar to previously published results obtained from other vertebrates. It is suggested that the dendrodendritic inhibitory pathway proposed for the mammalian olfactory bulb (Rall et al., 1966; Rall and Shepherd, 1968) is common to all vertebrates.", "contents": "Field potentials in the olfactory bulb of the rainbow trout (Salmo gairdneri): evidence for a dendrodendritic inhibitory pathway. Field potentials have been recorded from the olfactory bulb of the teleost fish, Salmo gairdneri. Stimulation of the olfactory nerve generates a complex wave pattern, which when recorded near the surface in the centre of the bulb consists of one positive and three negative components, P, N1, N2 and N3. The N1 has been attributed to depolarization of mitral cell dendrites, N2 to depolarization of the superficial dendrites of granules cells, and P to subsequent hyperpolarization of mitral cells. Possible origins of the N3 wave are discussed. Spikes from single units recorded in the mitral cell layer usually occur superimposed on the N1 wave, and single units recorded in the region of granule cells are usually found coincident with the trailling edge of the deep positive wave. With the exception of N3 the patterns of activity observed are very similar to previously published results obtained from other vertebrates. It is suggested that the dendrodendritic inhibitory pathway proposed for the mammalian olfactory bulb (Rall et al., 1966; Rall and Shepherd, 1968) is common to all vertebrates.", "PMID": 954891}
{"id": "pubmed23n0032_1409", "title": "Spontaneous activity of single neurons in the olfactory bulb of the rainbow trout (Salmo gairdneri) and its modulation by olfactory stimulation with amino acids.", "content": "Spontaneous action potentials recorded from 126 neurons in the olfactory bulb of Salmo gairdneri show a higher tendency towards bursting patterns of activity than those shown in previous reports about other fish. Granule cells and periglomerular cells are more likely to fire in bursts than mitral cells. Natural chemical stimulation of the olfactory mucosa with amino acid solutions produced a unique pattern of excitatory and inhibitory responses across all units. Chi-square values were calculated for stimulatory effectiveness between forty-five pairs of odours. L-serine and L-alanine consistently showed a high  degree of similarity with several other odours. The converse was true for GABA and L-histidine, although this pair had a high chi-square value when mutually compared. Enantiomeric pairs of amino acids were often found to have opposite stimulatory effects on bulbar units. These results are discussed in relation to the possible properties and configurations of odorant receptor sites for amino acids in the fish olfactory mucosa.", "contents": "Spontaneous activity of single neurons in the olfactory bulb of the rainbow trout (Salmo gairdneri) and its modulation by olfactory stimulation with amino acids. Spontaneous action potentials recorded from 126 neurons in the olfactory bulb of Salmo gairdneri show a higher tendency towards bursting patterns of activity than those shown in previous reports about other fish. Granule cells and periglomerular cells are more likely to fire in bursts than mitral cells. Natural chemical stimulation of the olfactory mucosa with amino acid solutions produced a unique pattern of excitatory and inhibitory responses across all units. Chi-square values were calculated for stimulatory effectiveness between forty-five pairs of odours. L-serine and L-alanine consistently showed a high  degree of similarity with several other odours. The converse was true for GABA and L-histidine, although this pair had a high chi-square value when mutually compared. Enantiomeric pairs of amino acids were often found to have opposite stimulatory effects on bulbar units. These results are discussed in relation to the possible properties and configurations of odorant receptor sites for amino acids in the fish olfactory mucosa.", "PMID": 954892}
{"id": "pubmed23n0032_1410", "title": "Maintained activity of cells in the three shrew's optic tract.", "content": "The maintained activity of different types of ganglion cells in the tree shrew's retina has been investigated in darkness and different adaptation luminances, with particular reference to on-centre sustained and transient cells. The firing rate of on-centre sustained cells rises with increasing luminance up to 5.10(-2) W/m2 (human photopic equivalent 5.10(4) cd/m2) whereas, for on-centre transient cells no simple relationship between luminance and activity could be found. The ratio of mean and standard deviation (regularity) of the interval length of sustained cells increases with light intensity, while in most transient cells a constant ratio is observed. Various kinds of interval histograms are observed for sustained units: exponential, gamma, bimodal and other types. Transient cells tend to fire in bursts with correspondent biomodal interval histograms. The first order serial correlation coefficient is positive for the majority of sustained cells and negative for most transient cells. It is argued that the final statistical properties of the maintained activity of retinal ganglion cells are mainly determined by the retinal circuitry between photoreceptors and ganglion cells.", "contents": "Maintained activity of cells in the three shrew's optic tract. The maintained activity of different types of ganglion cells in the tree shrew's retina has been investigated in darkness and different adaptation luminances, with particular reference to on-centre sustained and transient cells. The firing rate of on-centre sustained cells rises with increasing luminance up to 5.10(-2) W/m2 (human photopic equivalent 5.10(4) cd/m2) whereas, for on-centre transient cells no simple relationship between luminance and activity could be found. The ratio of mean and standard deviation (regularity) of the interval length of sustained cells increases with light intensity, while in most transient cells a constant ratio is observed. Various kinds of interval histograms are observed for sustained units: exponential, gamma, bimodal and other types. Transient cells tend to fire in bursts with correspondent biomodal interval histograms. The first order serial correlation coefficient is positive for the majority of sustained cells and negative for most transient cells. It is argued that the final statistical properties of the maintained activity of retinal ganglion cells are mainly determined by the retinal circuitry between photoreceptors and ganglion cells.", "PMID": 954893}
{"id": "pubmed23n0032_1411", "title": "Effects of frontal eye field stimulation upon activities of the lateral geniculate body of the cat.", "content": "Effects of electrical stimulation of the frontal eye field (FEF) upon activites of the lateral geniculate body (LG) were studied in encephale isole cats. In some experiments the effects were examined by recording field responses of the dorsal nucleus of LG (LGd) and the visual cortex (VC) to electrical stimulation of the optic chiasm (OX). Conditioning  repetitive stimulation of FEF exerted no significant effects on the r1 wave of LGd responses but had a facilitatory effect on the r2 wave. FEF-induced facilitation of VC responses was prominent in the late postsynaptic components. These effects had latencies of 50-100 msec and durations of 200-500 msec. Transection of the midbrain showed that most of the FEF-effect was not mediated via the brainstem reticular formation. Extracellular unitary recordings were made from 125 neurons, of which 91 were LGd neurons, 23 neurons of the caudal part of the thalamic reticular nucleus (TRc) and 11 neurons of the ventral nucleus of LG (LGv). In 30 to 87 LGd relay neurons FEF stimuli increased response probabilities to OX stimuli and their spontaneous discharges. These FEF-facilitated LGd neurons were distinguished from the non-affected ones in that the former had longer OX-latencies than the latter. The FEF-facilitated neurons probably correspond to \"X\" neurons of LGd. In 17 TRc neurons the effects were inhibitory. Their time courses were similar to those of the facilitation in the LGd relay neurons. Seven LGv neurons recieved facilitaroy effects from FEF. Among them 5 neurons showed short-latency (6.7-17 msec) responses to FEF single shocks. The FEF sites inducing conjugate lateral eye movements exerted stronger facilitatory effects than those inducing upward or centering eye movements did. It is suggested that the effects may subserve to cancel the inhibitory convergence onto X-cells just after saccadic eye movements so as to improve visual information transmission through LGd during the eye fixation.", "contents": "Effects of frontal eye field stimulation upon activities of the lateral geniculate body of the cat. Effects of electrical stimulation of the frontal eye field (FEF) upon activites of the lateral geniculate body (LG) were studied in encephale isole cats. In some experiments the effects were examined by recording field responses of the dorsal nucleus of LG (LGd) and the visual cortex (VC) to electrical stimulation of the optic chiasm (OX). Conditioning  repetitive stimulation of FEF exerted no significant effects on the r1 wave of LGd responses but had a facilitatory effect on the r2 wave. FEF-induced facilitation of VC responses was prominent in the late postsynaptic components. These effects had latencies of 50-100 msec and durations of 200-500 msec. Transection of the midbrain showed that most of the FEF-effect was not mediated via the brainstem reticular formation. Extracellular unitary recordings were made from 125 neurons, of which 91 were LGd neurons, 23 neurons of the caudal part of the thalamic reticular nucleus (TRc) and 11 neurons of the ventral nucleus of LG (LGv). In 30 to 87 LGd relay neurons FEF stimuli increased response probabilities to OX stimuli and their spontaneous discharges. These FEF-facilitated LGd neurons were distinguished from the non-affected ones in that the former had longer OX-latencies than the latter. The FEF-facilitated neurons probably correspond to \"X\" neurons of LGd. In 17 TRc neurons the effects were inhibitory. Their time courses were similar to those of the facilitation in the LGd relay neurons. Seven LGv neurons recieved facilitaroy effects from FEF. Among them 5 neurons showed short-latency (6.7-17 msec) responses to FEF single shocks. The FEF sites inducing conjugate lateral eye movements exerted stronger facilitatory effects than those inducing upward or centering eye movements did. It is suggested that the effects may subserve to cancel the inhibitory convergence onto X-cells just after saccadic eye movements so as to improve visual information transmission through LGd during the eye fixation.", "PMID": 954894}
{"id": "pubmed23n0032_1412", "title": "Dynamic properties of excitation and two-tone inhibition in the cochlear nucleus studied using amplitude-modulated tones.", "content": "The dynamic properties of excitation and two-tone inhibition in the cochlear nucleus were studied from extracellularly recorded unit responses to two simultaneously presented tones. One tone was presented at the unit's characteristic frequency, CF, the other at the unit's best inhibitory frequency, BIF. One or both of the tones were amplitude-modulated with pseudorandom noise. The system under study is in general nonlinear, but can be considered to function as a linear system for small changes in sound intensity around a certain operating point. The dynamic properties are likely to be different at different operating points. A suitable method for the study of dynamic properties of such a system employs tones that are amplitude-modulated with pseudorandom noise. In the present study, the dynamic properties were assessed by cross-correlating the unit discharge rate with the modulation. This was accomplished by computing the cross-covariance function between a period of noise and a period histogram of the discharges, the histogram being locked to the periodicity of the pseudorandom noise. In this way, it has been shown in previous works (Moller, 1973, 1974b), that the cross-covariance function is a valid approximation of the system's impulse response function at a certain sound intensity, provided the modulation is kept at a low value. In the present study the computed cross-covariance function is thus an approximation of the change in discharge rate of the cochlear nucleus units in response to a brief increase in stimulus intensity. As the response of the system under the given circumstances is approximately that of a linear system, the integrated cross-covariance is an approximation of the system's step response function, i.e the change in discharge rate that resulte from a hypothetical step increase in stimulus intensity. The results of the present study can be summarized as follows: 1. The impulse and step response functions computed from the responses to the modulated inhibitory tone of the great majority of units from which recording was made were found to be virtual mirror images of those obtained when the excitatory tone was modulated, the inhibitory response being somewhat smaller in amplitude than the excitatory. 2. When both tones were modulated simultaneously, the step response function was approximately the algebraic sum of the two responses obtained when the tones were modulated singly, further indicating that the system functions as a linear system when the stimulus amplitude is varied slightly around a certain operating point. 3. The shape of the cross-covariance functions is similar for all three stimulus situations, but varies with stimulus intensity and is different in different units. 4. The implication of the results is that the inhibition studied may either originate from the inhibition (suppression) seen in primary fibers or it may be the result of a true neural inhibition in the cochlear nucleus that occurs without any interneurons.", "contents": "Dynamic properties of excitation and two-tone inhibition in the cochlear nucleus studied using amplitude-modulated tones. The dynamic properties of excitation and two-tone inhibition in the cochlear nucleus were studied from extracellularly recorded unit responses to two simultaneously presented tones. One tone was presented at the unit's characteristic frequency, CF, the other at the unit's best inhibitory frequency, BIF. One or both of the tones were amplitude-modulated with pseudorandom noise. The system under study is in general nonlinear, but can be considered to function as a linear system for small changes in sound intensity around a certain operating point. The dynamic properties are likely to be different at different operating points. A suitable method for the study of dynamic properties of such a system employs tones that are amplitude-modulated with pseudorandom noise. In the present study, the dynamic properties were assessed by cross-correlating the unit discharge rate with the modulation. This was accomplished by computing the cross-covariance function between a period of noise and a period histogram of the discharges, the histogram being locked to the periodicity of the pseudorandom noise. In this way, it has been shown in previous works (Moller, 1973, 1974b), that the cross-covariance function is a valid approximation of the system's impulse response function at a certain sound intensity, provided the modulation is kept at a low value. In the present study the computed cross-covariance function is thus an approximation of the change in discharge rate of the cochlear nucleus units in response to a brief increase in stimulus intensity. As the response of the system under the given circumstances is approximately that of a linear system, the integrated cross-covariance is an approximation of the system's step response function, i.e the change in discharge rate that resulte from a hypothetical step increase in stimulus intensity. The results of the present study can be summarized as follows: 1. The impulse and step response functions computed from the responses to the modulated inhibitory tone of the great majority of units from which recording was made were found to be virtual mirror images of those obtained when the excitatory tone was modulated, the inhibitory response being somewhat smaller in amplitude than the excitatory. 2. When both tones were modulated simultaneously, the step response function was approximately the algebraic sum of the two responses obtained when the tones were modulated singly, further indicating that the system functions as a linear system when the stimulus amplitude is varied slightly around a certain operating point. 3. The shape of the cross-covariance functions is similar for all three stimulus situations, but varies with stimulus intensity and is different in different units. 4. The implication of the results is that the inhibition studied may either originate from the inhibition (suppression) seen in primary fibers or it may be the result of a true neural inhibition in the cochlear nucleus that occurs without any interneurons.", "PMID": 954895}
{"id": "pubmed23n0032_1413", "title": "The lateral reticular nucleus in the cat. V. Does collateral activation from the dorsal spinocerebellar tract occur?", "content": "1. The assertion made by Burton, Bloedel and Gregory (1971) that neurones in the mLRN (major portion of lateral reticular nucleus comprising its parvi- and magnocellular parts) receive collateral excitation from the dorsal spinocerebellar tract (DSCT) has been tested. The activation of mLRN neurones on stimulation of limb nerves was studied in preparations with the spinal cord interrupted at C3 sparing only the dorsal part of the ipsilateral lateral funiculus (iDLF preparations), only the ipsilateral ventral quadrant (iVQ preparations), or only the ipsilateral lateral and ventral funiculi (iLFVF preparations). 2. In iDLF preparations (with the DSCT intact) stimulation of limb nerves evoked responses in only occasional mLRN neurones. These responses could be attributed to activation from some dorsally located fibres belonging to the bilateral ventral flexor reflex tract (bVFRT) and to activation from a previously unknown path activated after a long latency from ipsilateral forelimb and hindlimb afferents. 3. In iLFVF preparations (with the DSCT intact) and iVQ preparations (with the DSCT interrupted) most mLRN neurones responsed to limb nerve stimulation. The responses in two preparations were similar and could be attributed largely or exclusively to activation from the bVFRT. 4 It is concluded that the DSCT does not activate any major group of mLRN neurones.", "contents": "The lateral reticular nucleus in the cat. V. Does collateral activation from the dorsal spinocerebellar tract occur? 1. The assertion made by Burton, Bloedel and Gregory (1971) that neurones in the mLRN (major portion of lateral reticular nucleus comprising its parvi- and magnocellular parts) receive collateral excitation from the dorsal spinocerebellar tract (DSCT) has been tested. The activation of mLRN neurones on stimulation of limb nerves was studied in preparations with the spinal cord interrupted at C3 sparing only the dorsal part of the ipsilateral lateral funiculus (iDLF preparations), only the ipsilateral ventral quadrant (iVQ preparations), or only the ipsilateral lateral and ventral funiculi (iLFVF preparations). 2. In iDLF preparations (with the DSCT intact) stimulation of limb nerves evoked responses in only occasional mLRN neurones. These responses could be attributed to activation from some dorsally located fibres belonging to the bilateral ventral flexor reflex tract (bVFRT) and to activation from a previously unknown path activated after a long latency from ipsilateral forelimb and hindlimb afferents. 3. In iLFVF preparations (with the DSCT intact) and iVQ preparations (with the DSCT interrupted) most mLRN neurones responsed to limb nerve stimulation. The responses in two preparations were similar and could be attributed largely or exclusively to activation from the bVFRT. 4 It is concluded that the DSCT does not activate any major group of mLRN neurones.", "PMID": 954897}
{"id": "pubmed23n0032_1414", "title": "Conduction velocity groupings among axons of cat retinal ganglion cells, and their relationship to retinal topography.", "content": "Three unresolved issues in the conduction velocity groupings among the axons of cat retinal ganglion cells have been investigated. These are (i) the degree of separation of the velocity ranges of the axons of X-and W-type ganglion cells; (ii) the question whether the velocities of X-and Y-cell axons (i.e. of t1 and t2 axons have faster velocities than contralaterally projecting axons. Evidence is presented that the velocity ranges of W- and X-cell axons are almost completely separate; that the velocities of X- and Y-cell axons are not graded with retinal eccentricity (except in the immediate vicinity of the area centralis) and that ipsilaterally projecting axons are not faster conducting that contralaterally projecting axons. These results provide a context for summarising present understanding of conduction velocity groupings among the axons of cat retinal ganglion cells.", "contents": "Conduction velocity groupings among axons of cat retinal ganglion cells, and their relationship to retinal topography. Three unresolved issues in the conduction velocity groupings among the axons of cat retinal ganglion cells have been investigated. These are (i) the degree of separation of the velocity ranges of the axons of X-and W-type ganglion cells; (ii) the question whether the velocities of X-and Y-cell axons (i.e. of t1 and t2 axons have faster velocities than contralaterally projecting axons. Evidence is presented that the velocity ranges of W- and X-cell axons are almost completely separate; that the velocities of X- and Y-cell axons are not graded with retinal eccentricity (except in the immediate vicinity of the area centralis) and that ipsilaterally projecting axons are not faster conducting that contralaterally projecting axons. These results provide a context for summarising present understanding of conduction velocity groupings among the axons of cat retinal ganglion cells.", "PMID": 954898}
{"id": "pubmed23n0032_1415", "title": "Functional characterization of primary vestibular afferents in the frog.", "content": "1. In order to more accurately identify the nature of the vestibular input to central neurons, the response properties of single semicircular canal and otolith units in the frog VIIth nerve were studied in curarized preparations. 2. An equation describing the response plane was calculated for each canal on the basis of null point measurements. These results show that the ipsilateral canal planes are orthogonal within 2-5 degrees, and the pairs of right-left synergists are essentially coplanar. A head position of 10-20 degrees maxilla nose up produces optimal horizontal canal and minimal vertical canal activation with horizontal rotation. 3. The frequency response of the horizontal canal was examined in the range 0.025-0.5 Hz. Comparatively shorter phase-lags and a 10 fold greater acceleration gain in this frequency range distinguish the frog from the mammalian species studied. 4. Otolithic responses were tonic, phasic-tonic, and phasic in nature. The preponderance of the latter two groups is stressed (94%). Tonic responses were proportional to the gravitational vector change. Phasic responses were proportional to velocity during transitions in head position and phase-led displacement (30-80%) with sinusoidal acceleration in roll and pitch. 5. Efferent vestibular neurons respond to rotation in the horizontal (usually Type III) as well as vertical planes. Responses in the vertical planes result from canal and/or otolithic input to these neurons indicating that the vestibular efferent system receives extensive multi-labyrinthine convergence.", "contents": "Functional characterization of primary vestibular afferents in the frog. 1. In order to more accurately identify the nature of the vestibular input to central neurons, the response properties of single semicircular canal and otolith units in the frog VIIth nerve were studied in curarized preparations. 2. An equation describing the response plane was calculated for each canal on the basis of null point measurements. These results show that the ipsilateral canal planes are orthogonal within 2-5 degrees, and the pairs of right-left synergists are essentially coplanar. A head position of 10-20 degrees maxilla nose up produces optimal horizontal canal and minimal vertical canal activation with horizontal rotation. 3. The frequency response of the horizontal canal was examined in the range 0.025-0.5 Hz. Comparatively shorter phase-lags and a 10 fold greater acceleration gain in this frequency range distinguish the frog from the mammalian species studied. 4. Otolithic responses were tonic, phasic-tonic, and phasic in nature. The preponderance of the latter two groups is stressed (94%). Tonic responses were proportional to the gravitational vector change. Phasic responses were proportional to velocity during transitions in head position and phase-led displacement (30-80%) with sinusoidal acceleration in roll and pitch. 5. Efferent vestibular neurons respond to rotation in the horizontal (usually Type III) as well as vertical planes. Responses in the vertical planes result from canal and/or otolithic input to these neurons indicating that the vestibular efferent system receives extensive multi-labyrinthine convergence.", "PMID": 954899}
{"id": "pubmed23n0032_1416", "title": "The in vivo inactivation of GABA and other inhibitory amino acids in the cat nervous system.", "content": "In cats anaesthetised with pentobarbitone, the effect of inhibitors of the in vitro cellular uptake of GABA were tested on the responses of single central neurones to GABA and other depressant amino acids. (4)- AND (-)-nepecotic acid, (4)-2,4-diaminobutyric acid (DABA) and 2,2-dimethyl-beta-alanine, enhanced the action of GABA on spinal, cerebellar and cerebral cortical neurones. In the spinal cord DABA, and to a less estent (-)-nipecotic acid, enhanced the action of beta-alanine, whereas the actions of glycine and taurine were unaffected by DABA and reduced by (-)-nipecotic acid. In the cerebellum and cerebral cortex, these two substances enhanced the action of GABA, usually to a greater extent than that of beta-alanine and taurine, although this specificity was not marked. The GABA-mediated basket cell inhibition of Purkinje cells in the cerebellum was unaffected by DABA and (-)-nipecotic acid, and neither substance appears suitable for determining the role of uptake processes in the inactivation of synapitcally released GABA. Quantitatively these in vivo results agree more closely with recent vitro uptake studies in cat tissue than the previously published data on rat cerebral cortex and dorsal root ganglia, and the observations provide further evidence for the importance of cellular uptake in maintaining low extraneuronal concentrations of inhibitory amino acid transmitters.", "contents": "The in vivo inactivation of GABA and other inhibitory amino acids in the cat nervous system. In cats anaesthetised with pentobarbitone, the effect of inhibitors of the in vitro cellular uptake of GABA were tested on the responses of single central neurones to GABA and other depressant amino acids. (4)- AND (-)-nepecotic acid, (4)-2,4-diaminobutyric acid (DABA) and 2,2-dimethyl-beta-alanine, enhanced the action of GABA on spinal, cerebellar and cerebral cortical neurones. In the spinal cord DABA, and to a less estent (-)-nipecotic acid, enhanced the action of beta-alanine, whereas the actions of glycine and taurine were unaffected by DABA and reduced by (-)-nipecotic acid. In the cerebellum and cerebral cortex, these two substances enhanced the action of GABA, usually to a greater extent than that of beta-alanine and taurine, although this specificity was not marked. The GABA-mediated basket cell inhibition of Purkinje cells in the cerebellum was unaffected by DABA and (-)-nipecotic acid, and neither substance appears suitable for determining the role of uptake processes in the inactivation of synapitcally released GABA. Quantitatively these in vivo results agree more closely with recent vitro uptake studies in cat tissue than the previously published data on rat cerebral cortex and dorsal root ganglia, and the observations provide further evidence for the importance of cellular uptake in maintaining low extraneuronal concentrations of inhibitory amino acid transmitters.", "PMID": 954900}
{"id": "pubmed23n0032_1417", "title": "Calcium uptake in preterminal central synapses: importance of mitochondria.", "content": "Energy dependent 45Ca2+ uptake in the synaptosomal preparation from guinea pig cortex has been investigated. 45Ca2+ uptake was stimulated by ATP, the absolute value of uptake being dependent on the extent of synaptosomal disruption caused by osmotic shock. A quantitative comparison of microsomal and mitochondrial ATP-dependent 45Ca2+ uptake showed that only mitochondria had a large enough capacity to account for the Ca uptake levels observed in the synaptosomal preparation. ATP-stimulated 45Ca2+ uptake in mitochondria, 'intact' and 'shocked' synaptosomes was inhibited by atractyloside, DNP, oligomycin and ruthenium red but unaffected by antimycin A and rotenone. This was interpreted as evidence that mitochondria were responsible for ATP-dependent synaptosomal Ca2+ uptake, the increase in uptake seen on osmotic lysis being due to the deocclusion of intraterminal mitochondria. Synaptosomal and mitochondrial 45Ca2+ uptake was also stimulated by the mitochondrial respiratory substrate glutamate; this uptake was sensitive to antimycin A, DNP, rotenone and ruthenium red but insensitive to atractyloside or oligomycin thus indicating it was of mitochondrial origin. No change in glutamate-dependent 45Ca2+ uptake was seen on osmotic lysis of the synaptosomes as the expected increase due to the release of occluded mitochondria was counterbalanced by the damaging effect of hypo-osmotic shock on the glutamate-stimulated 45Ca2+ uptake process.", "contents": "Calcium uptake in preterminal central synapses: importance of mitochondria. Energy dependent 45Ca2+ uptake in the synaptosomal preparation from guinea pig cortex has been investigated. 45Ca2+ uptake was stimulated by ATP, the absolute value of uptake being dependent on the extent of synaptosomal disruption caused by osmotic shock. A quantitative comparison of microsomal and mitochondrial ATP-dependent 45Ca2+ uptake showed that only mitochondria had a large enough capacity to account for the Ca uptake levels observed in the synaptosomal preparation. ATP-stimulated 45Ca2+ uptake in mitochondria, 'intact' and 'shocked' synaptosomes was inhibited by atractyloside, DNP, oligomycin and ruthenium red but unaffected by antimycin A and rotenone. This was interpreted as evidence that mitochondria were responsible for ATP-dependent synaptosomal Ca2+ uptake, the increase in uptake seen on osmotic lysis being due to the deocclusion of intraterminal mitochondria. Synaptosomal and mitochondrial 45Ca2+ uptake was also stimulated by the mitochondrial respiratory substrate glutamate; this uptake was sensitive to antimycin A, DNP, rotenone and ruthenium red but insensitive to atractyloside or oligomycin thus indicating it was of mitochondrial origin. No change in glutamate-dependent 45Ca2+ uptake was seen on osmotic lysis of the synaptosomes as the expected increase due to the release of occluded mitochondria was counterbalanced by the damaging effect of hypo-osmotic shock on the glutamate-stimulated 45Ca2+ uptake process.", "PMID": 954901}
{"id": "pubmed23n0032_1418", "title": "Hepatic and cerebral coenzyme A contents after intravenous injection of coenzyme A in rats.", "content": "Hepatic CoA concentrations and contents were significantly higher in rats having received i.v. CoA injections that in control rats. Maximum hepatic CoA concentrations were found 0.5-1 h after injection. In rat brain, no increase in CoA concentration was detected after i.v. injection of CoA.", "contents": "Hepatic and cerebral coenzyme A contents after intravenous injection of coenzyme A in rats. Hepatic CoA concentrations and contents were significantly higher in rats having received i.v. CoA injections that in control rats. Maximum hepatic CoA concentrations were found 0.5-1 h after injection. In rat brain, no increase in CoA concentration was detected after i.v. injection of CoA.", "PMID": 954956}
{"id": "pubmed23n0032_1419", "title": "[Isoenzymes of alkaline phosphatase - reference values in young people and effects of protein diet (author's transl)].", "content": "In 260 normal students, 20-25 years old, the variation in the activities of serum alkaline phosphatase and its isoenzymes with sex, ABO blood groups, and protein intake were studied. The values are on the whole higher in males than in females. The activity of the intestinal isoenzyme was higher in subjects taking protein-rich diet than in those taking protein poor diet.", "contents": "[Isoenzymes of alkaline phosphatase - reference values in young people and effects of protein diet (author's transl)]. In 260 normal students, 20-25 years old, the variation in the activities of serum alkaline phosphatase and its isoenzymes with sex, ABO blood groups, and protein intake were studied. The values are on the whole higher in males than in females. The activity of the intestinal isoenzyme was higher in subjects taking protein-rich diet than in those taking protein poor diet.", "PMID": 954957}
{"id": "pubmed23n0032_1420", "title": "Cholesterol esterification activities in the intestines and pancreas of the albino rat.", "content": "Cholesterol esterification activities in intestines and pancreas are much greater with unsaturated fatty acids than with the saturated ones; the maximum activity is with arachidonic acid in intestines and with oleic acid in pancreas. The pancreatic cholesterol esterification activity is higher than the intestinal one.", "contents": "Cholesterol esterification activities in the intestines and pancreas of the albino rat. Cholesterol esterification activities in intestines and pancreas are much greater with unsaturated fatty acids than with the saturated ones; the maximum activity is with arachidonic acid in intestines and with oleic acid in pancreas. The pancreatic cholesterol esterification activity is higher than the intestinal one.", "PMID": 954958}
{"id": "pubmed23n0032_1421", "title": "Lipolytic response of \"diabetic\" mice (db/db) to isoproterenol and propranolol in vivo.", "content": "The genetically diabetic and obese db/db mice responded lipolytically to isoproterenol and propranolol similarly to normal mice in vivo. However, considering the large amount of triglyceride in a db/db mouse, we conclude that the in vivo response of db/db adipose tissue is deficient in magnitude.", "contents": "Lipolytic response of \"diabetic\" mice (db/db) to isoproterenol and propranolol in vivo. The genetically diabetic and obese db/db mice responded lipolytically to isoproterenol and propranolol similarly to normal mice in vivo. However, considering the large amount of triglyceride in a db/db mouse, we conclude that the in vivo response of db/db adipose tissue is deficient in magnitude.", "PMID": 954959}
{"id": "pubmed23n0032_1422", "title": "The effects of various agents in vitro on homocarnosine-carnosine synthetase from rat brain.", "content": "The present paper reports the first evidence that homocarnosine-carnosine synthetase from rat brain requires free sulfhydryl groups for activity. The activity of the synthetase can be stabilized by dithioerythritol and inhibited strongly by Cu2+, Cd2+, Hg2+, Zn2+, and to a lesser extent by Ca2+, Ni2+, Li, chlorpromazine, alpha-methyl DOPA and norepinephrine at the concentrations tested.", "contents": "The effects of various agents in vitro on homocarnosine-carnosine synthetase from rat brain. The present paper reports the first evidence that homocarnosine-carnosine synthetase from rat brain requires free sulfhydryl groups for activity. The activity of the synthetase can be stabilized by dithioerythritol and inhibited strongly by Cu2+, Cd2+, Hg2+, Zn2+, and to a lesser extent by Ca2+, Ni2+, Li, chlorpromazine, alpha-methyl DOPA and norepinephrine at the concentrations tested.", "PMID": 954961}
{"id": "pubmed23n0032_1423", "title": "Effects of pre- and simultaneous treatment with urea on the induction of chromatid aberrations in Vicia faba.", "content": "Pre- and simultaneous treatments of Vicia faba root tip meristems with urea and ethyl alcohol, mitomycin C, maleic hydrazide, 3H-thymidine and X-rays, respectively, were found to result in mutagen-specific changes of the spectrum and yield of induced chromatid aberrations.", "contents": "Effects of pre- and simultaneous treatment with urea on the induction of chromatid aberrations in Vicia faba. Pre- and simultaneous treatments of Vicia faba root tip meristems with urea and ethyl alcohol, mitomycin C, maleic hydrazide, 3H-thymidine and X-rays, respectively, were found to result in mutagen-specific changes of the spectrum and yield of induced chromatid aberrations.", "PMID": 954962}
{"id": "pubmed23n0032_1424", "title": "Nuclear projections in tumour cells and large chromosome markers.", "content": "The application of banding techniques on cytological smears from pleural effusion in a case of histiocytic sarcoma has provided direct evidence for correspondence between nuclear projections in tumour cells and extra large chromosome markers observed in the neoplastic karyotype obtained by direct preparations.", "contents": "Nuclear projections in tumour cells and large chromosome markers. The application of banding techniques on cytological smears from pleural effusion in a case of histiocytic sarcoma has provided direct evidence for correspondence between nuclear projections in tumour cells and extra large chromosome markers observed in the neoplastic karyotype obtained by direct preparations.", "PMID": 954963}
{"id": "pubmed23n0032_1425", "title": "Genetic variation in constant environments.", "content": "Allozymic variation in proteins encoded by 32 gene loci was analyzed electrophoretically in 64 specimens from 6 localities representing 2 species of the spadefoot toads Pelobates syriacus and P. cultripes from Israel and Portugal, respectively. Out of the 32 loci examined, Esterase-1 was the only locus that proved strongly polymorphic in all 6 localities and in the 2 species. The pattern of genetic variation in Pelobates is best explained by the environmental variability model. Selection for homozygosity as an adaptive strategy seems to operate in the relatively constant and narrow subterranean niche.", "contents": "Genetic variation in constant environments. Allozymic variation in proteins encoded by 32 gene loci was analyzed electrophoretically in 64 specimens from 6 localities representing 2 species of the spadefoot toads Pelobates syriacus and P. cultripes from Israel and Portugal, respectively. Out of the 32 loci examined, Esterase-1 was the only locus that proved strongly polymorphic in all 6 localities and in the 2 species. The pattern of genetic variation in Pelobates is best explained by the environmental variability model. Selection for homozygosity as an adaptive strategy seems to operate in the relatively constant and narrow subterranean niche.", "PMID": 954964}
{"id": "pubmed23n0032_1426", "title": "The behavior of alloxan in the Hill reaction.", "content": "Hill activity induced by alloxan is characterized by non-stoichiometric oxygen evolution and cyclic electron flow due to autoxidation of dialuric acid and alloxantin, the reduction products of alloxan.", "contents": "The behavior of alloxan in the Hill reaction. Hill activity induced by alloxan is characterized by non-stoichiometric oxygen evolution and cyclic electron flow due to autoxidation of dialuric acid and alloxantin, the reduction products of alloxan.", "PMID": 954965}
{"id": "pubmed23n0032_1427", "title": "Poly(A) associated RNA from mitochondria and microsomes of rat brain.", "content": "Rat brain mitochondria contain a significant proportion of poly(A) associated RNA which is higher than that found in microsomes from the same source. When steady state poly(A) RNA of brain mitochondria was analyzed by microelectrophoresis, it displayed a characteristic separation pattern with a large amount of \"free\" poly(A).", "contents": "Poly(A) associated RNA from mitochondria and microsomes of rat brain. Rat brain mitochondria contain a significant proportion of poly(A) associated RNA which is higher than that found in microsomes from the same source. When steady state poly(A) RNA of brain mitochondria was analyzed by microelectrophoresis, it displayed a characteristic separation pattern with a large amount of \"free\" poly(A).", "PMID": 954966}
{"id": "pubmed23n0032_1428", "title": "Spontaneous thermoregulatory oscillations in cutaneous efferent sympathetic activity.", "content": "Patterns of changes in cutaneous efferent sympathetic activity which have previously been shown to occur during experimental manipulation of the temperature of various thermosensitive body sites, have now been shown to accompany spontaneous thermoregulatory activity. That is, under thermoneural conditions, concurrent spontaneous oscillations in skin blood flow and efferent sympathetic activity were observed.", "contents": "Spontaneous thermoregulatory oscillations in cutaneous efferent sympathetic activity. Patterns of changes in cutaneous efferent sympathetic activity which have previously been shown to occur during experimental manipulation of the temperature of various thermosensitive body sites, have now been shown to accompany spontaneous thermoregulatory activity. That is, under thermoneural conditions, concurrent spontaneous oscillations in skin blood flow and efferent sympathetic activity were observed.", "PMID": 954967}
{"id": "pubmed23n0032_1429", "title": "The time course of plasma enzyme changes accompanying skeletal muscle stimulation.", "content": "30 min electrical stimulation of hind limb skeletal muscle in nembutal anaesthetised dogs was accompanied by increases in arterial haematocrit, plasma GOT and plasma LDH, which were almost completed within the first 10 min of stimulation. This fast response indicated that a rapid change in either the entry and/or clearance of enzyme from the plasma must have occurred.", "contents": "The time course of plasma enzyme changes accompanying skeletal muscle stimulation. 30 min electrical stimulation of hind limb skeletal muscle in nembutal anaesthetised dogs was accompanied by increases in arterial haematocrit, plasma GOT and plasma LDH, which were almost completed within the first 10 min of stimulation. This fast response indicated that a rapid change in either the entry and/or clearance of enzyme from the plasma must have occurred.", "PMID": 954969}
{"id": "pubmed23n0032_1430", "title": "On the role of the decreased renal vascular resistance in the mechanism of volume natriuresis.", "content": "Acute hypophysectomy (AH) prevented the increase of the cardiac output, renal cortical blood flow (e.g. the decrease of the renal vascular resistance) and renal sodium excretion during the ECFV expansion. The non-occurrence of natriuresis in AH rats is suggested as being partly in causal relation to the inability to decrease the renal vascular resistance and thus to increase the peritubular hydrostatic pressure.", "contents": "On the role of the decreased renal vascular resistance in the mechanism of volume natriuresis. Acute hypophysectomy (AH) prevented the increase of the cardiac output, renal cortical blood flow (e.g. the decrease of the renal vascular resistance) and renal sodium excretion during the ECFV expansion. The non-occurrence of natriuresis in AH rats is suggested as being partly in causal relation to the inability to decrease the renal vascular resistance and thus to increase the peritubular hydrostatic pressure.", "PMID": 954970}
{"id": "pubmed23n0032_1431", "title": "A presynaptic effect of whole venom of Dendroaspis jamesoni on the hemidiaphragm of rat.", "content": "Dendroaspis jamesoni venom in a dose of 12.5 mug/ml restricts the uptake of Ca+ leading to inhibition of release of Ach from the motor nerve terminals of the hemidiaphragms of rats.", "contents": "A presynaptic effect of whole venom of Dendroaspis jamesoni on the hemidiaphragm of rat. Dendroaspis jamesoni venom in a dose of 12.5 mug/ml restricts the uptake of Ca+ leading to inhibition of release of Ach from the motor nerve terminals of the hemidiaphragms of rats.", "PMID": 954971}
{"id": "pubmed23n0032_1432", "title": "The influence of salt intake on glomerular count in compensatory kidney hypertrophy in rats of different ages.", "content": "Drinking saline instead of water elevated the glomerular count in hypertrophied kidneys of rats uninephrectomized as adults. No changes occurred in glomerular concentration in kidney tissue indicating a more marked increase of other kidney structures. This procedure was ineffective in immature animals.", "contents": "The influence of salt intake on glomerular count in compensatory kidney hypertrophy in rats of different ages. Drinking saline instead of water elevated the glomerular count in hypertrophied kidneys of rats uninephrectomized as adults. No changes occurred in glomerular concentration in kidney tissue indicating a more marked increase of other kidney structures. This procedure was ineffective in immature animals.", "PMID": 954972}
{"id": "pubmed23n0032_1433", "title": "Exocrine pancreatic enzymes in cycloheximide treated rats.", "content": "Cycloheximide, even in a dose of 0.25 mg/kg administered s.c. to rats stimulated by pancreozymin and secretin, inhibited lipase activity in pancreatic juice. Lipase activity in serum of control animals was inhibited by cycloheximide. The secretion of trypsin and chymotrypsin was also decreased.", "contents": "Exocrine pancreatic enzymes in cycloheximide treated rats. Cycloheximide, even in a dose of 0.25 mg/kg administered s.c. to rats stimulated by pancreozymin and secretin, inhibited lipase activity in pancreatic juice. Lipase activity in serum of control animals was inhibited by cycloheximide. The secretion of trypsin and chymotrypsin was also decreased.", "PMID": 954974}
{"id": "pubmed23n0032_1434", "title": "[3H]-Noncocaine and [3H]-pseudococaine: effect of N-demethylation and C2-epimerization of cocaine on its pharmacokinetics in the rat.", "content": "After i.v. injections of cocaine, norcocaine, pseudococaine to the rat, the T 1/2 in brain were 0.4, 0.6, 0.2 h respectively and in plasma 0.4, 0.5, 0.2 h respectively. Benzoylnorecgonine and norecgonine were the metabolites of norcocaine in brain. Pseudonorcocaine, pseudobenzoylnorecgonine, pseudobenzoylecgonine and pseudoecgonine were the metabolites of pseudococaine in rat brain. Benzolynorecgonine and pseudobenzoylecgonine had potent stimulant activity intracisternally in the rats.", "contents": "[3H]-Noncocaine and [3H]-pseudococaine: effect of N-demethylation and C2-epimerization of cocaine on its pharmacokinetics in the rat. After i.v. injections of cocaine, norcocaine, pseudococaine to the rat, the T 1/2 in brain were 0.4, 0.6, 0.2 h respectively and in plasma 0.4, 0.5, 0.2 h respectively. Benzoylnorecgonine and norecgonine were the metabolites of norcocaine in brain. Pseudonorcocaine, pseudobenzoylnorecgonine, pseudobenzoylecgonine and pseudoecgonine were the metabolites of pseudococaine in rat brain. Benzolynorecgonine and pseudobenzoylecgonine had potent stimulant activity intracisternally in the rats.", "PMID": 954975}
{"id": "pubmed23n0032_1435", "title": "On the mechanism of pyrogenic action of ricin.", "content": "The incubation of rabbit white blood cells with ricin, the toxic protein of castor oil seeds, leads to the production of endogenous pyrogens. This induction can be inhibited by the antibiotics actinomycin D or cycloheximide. The results are discussed in terms of disturbed corticosteroid- and Mg2+-levels.", "contents": "On the mechanism of pyrogenic action of ricin. The incubation of rabbit white blood cells with ricin, the toxic protein of castor oil seeds, leads to the production of endogenous pyrogens. This induction can be inhibited by the antibiotics actinomycin D or cycloheximide. The results are discussed in terms of disturbed corticosteroid- and Mg2+-levels.", "PMID": 954976}
{"id": "pubmed23n0032_1436", "title": "Monamines in brain and urine of rats with hereditary hypothalamic diabetes insipidus.", "content": "Monoamine levels in brain and urine of homozygous and heterozygous diabetes insipidus (DI) rats (Brattleboro strain) were assessed. Homozygous DI rats had a higher whole brain content of serotonin than their heterozygous littermates. However, when corrected for differences in brain weight, homozygous DI also appeared to have higher brain concentrations of noradrenaline, tyrosine and GABA. The total 24 h excretion of all amines and their precursors was greater in the homozygous than in the heterozygous rats.", "contents": "Monamines in brain and urine of rats with hereditary hypothalamic diabetes insipidus. Monoamine levels in brain and urine of homozygous and heterozygous diabetes insipidus (DI) rats (Brattleboro strain) were assessed. Homozygous DI rats had a higher whole brain content of serotonin than their heterozygous littermates. However, when corrected for differences in brain weight, homozygous DI also appeared to have higher brain concentrations of noradrenaline, tyrosine and GABA. The total 24 h excretion of all amines and their precursors was greater in the homozygous than in the heterozygous rats.", "PMID": 954977}
{"id": "pubmed23n0032_1437", "title": "Inhibition of high affinity uptake of GABA by branched fatty acids.", "content": "Several branched fatty acids including an antiepileptic agent nDPA were tested as potential inhibitors of high affinity uptake of GABA by brain slices and synaptosomes. Only three compounds (2-butyl-3-propylhexanoic acid, 5-propyloctanoic acid, 2-propylpenten-2-oic acid) were found to be relatively weak inhibitors of the uptake system. There was no correlation between anticonvulsant properties of the branched fatty acids and their potencies as inhibitors of high affinity uptake of GABA.", "contents": "Inhibition of high affinity uptake of GABA by branched fatty acids. Several branched fatty acids including an antiepileptic agent nDPA were tested as potential inhibitors of high affinity uptake of GABA by brain slices and synaptosomes. Only three compounds (2-butyl-3-propylhexanoic acid, 5-propyloctanoic acid, 2-propylpenten-2-oic acid) were found to be relatively weak inhibitors of the uptake system. There was no correlation between anticonvulsant properties of the branched fatty acids and their potencies as inhibitors of high affinity uptake of GABA.", "PMID": 954978}
{"id": "pubmed23n0032_1438", "title": "The electrophysiological effects of ionophore X-537A on cardiac purkinje fibres.", "content": "Using classical microelectrode techniques in canine cardiac Purkinje fibres, calcium ionophore X-537A was shown to shorten the action potential, hyperpolarizing the membrane and lowering the plateau, suggesting that intracellular calcium controls membrane permeability to potassium in this preparation.", "contents": "The electrophysiological effects of ionophore X-537A on cardiac purkinje fibres. Using classical microelectrode techniques in canine cardiac Purkinje fibres, calcium ionophore X-537A was shown to shorten the action potential, hyperpolarizing the membrane and lowering the plateau, suggesting that intracellular calcium controls membrane permeability to potassium in this preparation.", "PMID": 954979}
{"id": "pubmed23n0032_1439", "title": "Ultrastructural study of binucleation in cells of the rat adrenal glomerular zone after a prolonged low-sodium diet.", "content": "Binucleate cells have been found in the glomerular zone of the adrenal cortex in rats subjected to low-sodium diets. By considering the various possibilities for their production, both the findings of nuclei in process of constriction and nuclei identical in form, confronted and smaller in size than those of neighbour cells, are in agreement with an amitotic nuclear division as the possible mechanism for the formation of these cells.", "contents": "Ultrastructural study of binucleation in cells of the rat adrenal glomerular zone after a prolonged low-sodium diet. Binucleate cells have been found in the glomerular zone of the adrenal cortex in rats subjected to low-sodium diets. By considering the various possibilities for their production, both the findings of nuclei in process of constriction and nuclei identical in form, confronted and smaller in size than those of neighbour cells, are in agreement with an amitotic nuclear division as the possible mechanism for the formation of these cells.", "PMID": 954980}
{"id": "pubmed23n0032_1440", "title": "Epidermal growth factor enhancement of skin tumor induction in mice.", "content": "Subcutaneous injection of epidermal growth factor 1. significantly shortened the latency period for the appearance of methylcholanthrene induced skin tumors and 2. increased the average number of papillomas elicited per mouse in both the Swiss Webster and C3HeB/FeJ strains.", "contents": "Epidermal growth factor enhancement of skin tumor induction in mice. Subcutaneous injection of epidermal growth factor 1. significantly shortened the latency period for the appearance of methylcholanthrene induced skin tumors and 2. increased the average number of papillomas elicited per mouse in both the Swiss Webster and C3HeB/FeJ strains.", "PMID": 954982}
{"id": "pubmed23n0032_1441", "title": "Calcium and magnesium deficiency-induced atrophy of muscle and calcium accumulation in the spinal cord.", "content": "After severe dietary calcium-magnesium deficiency in rats, succinic dehydrogenase and acetylcholinesterase enzyme activity of gastrocnemius muscle showed a neurogenic atrophy. This alteration was associated with a high concentration of calcium in the spinal cord.", "contents": "Calcium and magnesium deficiency-induced atrophy of muscle and calcium accumulation in the spinal cord. After severe dietary calcium-magnesium deficiency in rats, succinic dehydrogenase and acetylcholinesterase enzyme activity of gastrocnemius muscle showed a neurogenic atrophy. This alteration was associated with a high concentration of calcium in the spinal cord.", "PMID": 954983}
{"id": "pubmed23n0032_1442", "title": "Sister chromatid differential staining pattern in prematurely condensed chromosomes.", "content": "Application of sister chromatid differential (SCD) procedure on G1, S and G2 prematurely condensed chromosomes (PCC) of cells in the second and third cycle of DNA replication in medium containing BrdU reveals differential staining patterns characteristic of their respective stages in the cell cycle. These findings also suggest a structural similarity between PCC and metaphase chromosomes.", "contents": "Sister chromatid differential staining pattern in prematurely condensed chromosomes. Application of sister chromatid differential (SCD) procedure on G1, S and G2 prematurely condensed chromosomes (PCC) of cells in the second and third cycle of DNA replication in medium containing BrdU reveals differential staining patterns characteristic of their respective stages in the cell cycle. These findings also suggest a structural similarity between PCC and metaphase chromosomes.", "PMID": 954984}
{"id": "pubmed23n0032_1443", "title": "Chemical modification of genetic damage from continuous irradiation in mice.", "content": "It has been demonstrated that Adeturon (S-2-aminoethyl-iso-thiuronium adenosine triphosphate) showed a marked protective effect against translocations induction in mice germ cells after chronic gamma-or neutron-irradiation.", "contents": "Chemical modification of genetic damage from continuous irradiation in mice. It has been demonstrated that Adeturon (S-2-aminoethyl-iso-thiuronium adenosine triphosphate) showed a marked protective effect against translocations induction in mice germ cells after chronic gamma-or neutron-irradiation.", "PMID": 954985}
{"id": "pubmed23n0032_1444", "title": "Unstable L-forms of micrococci in human foetal blood.", "content": "In human foetal blood the presence of Micrococcaceae in the unstable L-form, probably taking origin from the placental transmission of minimal reproductive units, has been recognized by means of microscopic and cultural methods.", "contents": "Unstable L-forms of micrococci in human foetal blood. In human foetal blood the presence of Micrococcaceae in the unstable L-form, probably taking origin from the placental transmission of minimal reproductive units, has been recognized by means of microscopic and cultural methods.", "PMID": 954986}
{"id": "pubmed23n0032_1445", "title": "High levels of free fatty acids and their esters in lymphoid cells resistant to cortisone or cyclophosphamide.", "content": "The lymphoid cells from thymus, spleen or mesenteric lymph node of mice treated with hydrocortisone or cyclophosphamide contained the significantly high levels of free fatty acids, triglycerides and cholesterol esters as compared to the corresponding cells from untreated animals.", "contents": "High levels of free fatty acids and their esters in lymphoid cells resistant to cortisone or cyclophosphamide. The lymphoid cells from thymus, spleen or mesenteric lymph node of mice treated with hydrocortisone or cyclophosphamide contained the significantly high levels of free fatty acids, triglycerides and cholesterol esters as compared to the corresponding cells from untreated animals.", "PMID": 954987}
{"id": "pubmed23n0032_1446", "title": "Distribution of the A blood-group activity in porcine serum.", "content": "The A blood-group activity of pig serum is bound to a lipid, in some cases also to a nonlipid fraction. The major lipidic A activity (roughly 50%) is carried with the HDL class, while the VLDL and LDL classes contain roughly 25% each.", "contents": "Distribution of the A blood-group activity in porcine serum. The A blood-group activity of pig serum is bound to a lipid, in some cases also to a nonlipid fraction. The major lipidic A activity (roughly 50%) is carried with the HDL class, while the VLDL and LDL classes contain roughly 25% each.", "PMID": 954988}
{"id": "pubmed23n0032_1447", "title": "The effect of renal hydrodynamics on immune complex deposition.", "content": "The role of renal hydrodynamics on renal deposition of immune complexes was evaluated in acute serum sickness. Using i.v. radiolabelled antigen in rabbits under a variety of hydrodynamic alterations, these studies suggested that although intrarenal hydrodynamics influence renal deposition of immune complexes factors other than intrarenal hydrostatic pressure may be important.", "contents": "The effect of renal hydrodynamics on immune complex deposition. The role of renal hydrodynamics on renal deposition of immune complexes was evaluated in acute serum sickness. Using i.v. radiolabelled antigen in rabbits under a variety of hydrodynamic alterations, these studies suggested that although intrarenal hydrodynamics influence renal deposition of immune complexes factors other than intrarenal hydrostatic pressure may be important.", "PMID": 954990}
{"id": "pubmed23n0032_1448", "title": "Subcellular steroid distribution in the rat adrenal cortex.", "content": "The subcellular distribution of main steroids (pregnenolone, progesterone, corticosterone) and of cholesterol -- this last after correction according to exchanges occuring during differential centrifugation -- is presented in the rat adrenal cortex. The distribution patterns are not necessarily the same as established according to the localization of hydroxylating enzymes.", "contents": "Subcellular steroid distribution in the rat adrenal cortex. The subcellular distribution of main steroids (pregnenolone, progesterone, corticosterone) and of cholesterol -- this last after correction according to exchanges occuring during differential centrifugation -- is presented in the rat adrenal cortex. The distribution patterns are not necessarily the same as established according to the localization of hydroxylating enzymes.", "PMID": 954991}
{"id": "pubmed23n0032_1449", "title": "Effects of thyroxine and exercise on the glandular and plasma levels of corticosterone in the male rat.", "content": "The results presented here demonstrates that the thyroid gland is essential for normal corticosterone production. They further show that exercise stimulates this production whether the thyroid gland is present or not. The release or metabolism of corticosterone seems dependent upon an intact thyroid gland since plasma levels of corticosterone are decreased during exercise if the thyroid is absent. The administration of thyroxine is not sufficient to renew these levels.", "contents": "Effects of thyroxine and exercise on the glandular and plasma levels of corticosterone in the male rat. The results presented here demonstrates that the thyroid gland is essential for normal corticosterone production. They further show that exercise stimulates this production whether the thyroid gland is present or not. The release or metabolism of corticosterone seems dependent upon an intact thyroid gland since plasma levels of corticosterone are decreased during exercise if the thyroid is absent. The administration of thyroxine is not sufficient to renew these levels.", "PMID": 954993}
{"id": "pubmed23n0032_1450", "title": "Testosterone in human saliva.", "content": "Testosterone has been detected in whole human saliva. Levels averaged (+/- SE) 295 +/- 36 and 195 +/- 25 pg/ml in adult males and females, respectively, and usually were undetectable in children. In adult males, the excretion of testosterone in saliva appeared to follow a circadian rhythm.", "contents": "Testosterone in human saliva. Testosterone has been detected in whole human saliva. Levels averaged (+/- SE) 295 +/- 36 and 195 +/- 25 pg/ml in adult males and females, respectively, and usually were undetectable in children. In adult males, the excretion of testosterone in saliva appeared to follow a circadian rhythm.", "PMID": 954994}
{"id": "pubmed23n0032_1451", "title": "Monoamine oxidase localization in the ependyma and infundibular recess in the catfish Clarias batrachus and its probable significance.", "content": "The presence of monoamine oxidase (MAO) in the cerebrospinal fluid (CSF) and MAO positive tracts bridging the CSF and the subependyma strongly suggest the involvement of CSF in the neuroendocrine control of hypophysial function.", "contents": "Monoamine oxidase localization in the ependyma and infundibular recess in the catfish Clarias batrachus and its probable significance. The presence of monoamine oxidase (MAO) in the cerebrospinal fluid (CSF) and MAO positive tracts bridging the CSF and the subependyma strongly suggest the involvement of CSF in the neuroendocrine control of hypophysial function.", "PMID": 954995}
{"id": "pubmed23n0032_1452", "title": "New karyological data of Rhinoderma: the chromosomes of Rhinoderma rufum.", "content": "The chromosomes of the Chilean frog Rhinoderma rufum are described for the first time. This chromosome set is compared with the karyotype of R. darwinii. The importance of the karyological data applied to the phylogeny and systematics of this genus are discussed. A tentative hypothesis of karyological evolution of Rhinoderma is given.", "contents": "New karyological data of Rhinoderma: the chromosomes of Rhinoderma rufum. The chromosomes of the Chilean frog Rhinoderma rufum are described for the first time. This chromosome set is compared with the karyotype of R. darwinii. The importance of the karyological data applied to the phylogeny and systematics of this genus are discussed. A tentative hypothesis of karyological evolution of Rhinoderma is given.", "PMID": 954998}
{"id": "pubmed23n0032_1453", "title": "Thermodynamic aspects of development for Tenebrio molitor L.", "content": "Predictions of the thermodynamics of irreversible processes are tested for the development and aging of an insect. Specific heat production and specific respiration rate decrease towards a steady state with deviations for the time of hatching of the imago.", "contents": "Thermodynamic aspects of development for Tenebrio molitor L. Predictions of the thermodynamics of irreversible processes are tested for the development and aging of an insect. Specific heat production and specific respiration rate decrease towards a steady state with deviations for the time of hatching of the imago.", "PMID": 954999}
{"id": "pubmed23n0032_1454", "title": "Growth and development of the American bollworm Heliothis armigera Hubn. under laboratory mass rearing conditions.", "content": "A kidney bean meal diet was the most satisfactory laboratory diet for the larvae of the American bollworm Heliothis armigera Hubn. Optimum rates of survival (63.5%) occurred and the larval growth was better than that of larvae reared on castor oil plant leaves. The pupal weight and the fecundity of the resulting adults were also much better than those on the control host plant.", "contents": "Growth and development of the American bollworm Heliothis armigera Hubn. under laboratory mass rearing conditions. A kidney bean meal diet was the most satisfactory laboratory diet for the larvae of the American bollworm Heliothis armigera Hubn. Optimum rates of survival (63.5%) occurred and the larval growth was better than that of larvae reared on castor oil plant leaves. The pupal weight and the fecundity of the resulting adults were also much better than those on the control host plant.", "PMID": 955000}
{"id": "pubmed23n0032_1455", "title": "Electric organ discharges of the weakly electric fish Gymnarchus niloticus (Mormyriformes) in its natural habitat.", "content": "Electric organ discharges (EODs) of Gymnarchus niloticus in its natural habitat (Chari River, Chad Basin) and accompanying ecological data (pH, conductivity, temperature, turbidity, O2 dissolved) were recorded. The EOD frequencies ranged from 204 to 313 Hz (day) and 196-326 Hz (night). In social swimming the range of interfish EOD frequency differences was from 4 to 82 Hz. The EOD frequency seems to decrease with the age of the fish.", "contents": "Electric organ discharges of the weakly electric fish Gymnarchus niloticus (Mormyriformes) in its natural habitat. Electric organ discharges (EODs) of Gymnarchus niloticus in its natural habitat (Chari River, Chad Basin) and accompanying ecological data (pH, conductivity, temperature, turbidity, O2 dissolved) were recorded. The EOD frequencies ranged from 204 to 313 Hz (day) and 196-326 Hz (night). In social swimming the range of interfish EOD frequency differences was from 4 to 82 Hz. The EOD frequency seems to decrease with the age of the fish.", "PMID": 955001}
{"id": "pubmed23n0032_1456", "title": "Primary nucleolus and amphinucleoli in the oocytes of Patella coerulea L. (Moll. Gast.).", "content": "Electron microscope observations show in the primary nucleolus some granulations with dimensions less 70 A than those of the amphinucleoli (90 A). Even though the primary nucleolus has a high RNA content, this has not a very active turnover except at the periphery, probably in relation to the emission of 'daughter-nucleoli'. The amphinucleoli, even though they do not have RNA which is cytochemically discloseable, possess, however, RNA at a very high rate of turnover.", "contents": "Primary nucleolus and amphinucleoli in the oocytes of Patella coerulea L. (Moll. Gast.). Electron microscope observations show in the primary nucleolus some granulations with dimensions less 70 A than those of the amphinucleoli (90 A). Even though the primary nucleolus has a high RNA content, this has not a very active turnover except at the periphery, probably in relation to the emission of 'daughter-nucleoli'. The amphinucleoli, even though they do not have RNA which is cytochemically discloseable, possess, however, RNA at a very high rate of turnover.", "PMID": 955002}
{"id": "pubmed23n0032_1457", "title": "Relation of efferent impulse activity in splenic nerve to reflexly induced reactions of resistance and capacitance vessels to spleen.", "content": "Constrictory responses of splenic resistance vessels arising under pressor reflexes were abolished by hexonium (2 mg/kg) as well as the high amplitude (above 15 muV) impulses in sympathetic splenic nerves. Constrictory and dilatory responses of splenic capacitance vessels were preserved after administration of the same dose of hexonium and correlated as to the directivity with the changes of the low amplitude (15 muV and lower) impulsation in the splenic nerve.", "contents": "Relation of efferent impulse activity in splenic nerve to reflexly induced reactions of resistance and capacitance vessels to spleen. Constrictory responses of splenic resistance vessels arising under pressor reflexes were abolished by hexonium (2 mg/kg) as well as the high amplitude (above 15 muV) impulses in sympathetic splenic nerves. Constrictory and dilatory responses of splenic capacitance vessels were preserved after administration of the same dose of hexonium and correlated as to the directivity with the changes of the low amplitude (15 muV and lower) impulsation in the splenic nerve.", "PMID": 955004}
{"id": "pubmed23n0032_1458", "title": "'Binding' of glutamate and asparatate to synaptosomal fractions of six regions of the feline brain.", "content": "The order of potency of 'binding' of both glutamate and aspartate to synaptosomal fractions of brain regions was: cerebellar cortex greater than caudate nucleus greater than or equal to cerebral cortex greater than medulla approximately pons greater than corona radiata. Glutamate was bound to a greater extent than aspartate to particles of all regions studied, except for cerebral cortex.", "contents": "'Binding' of glutamate and asparatate to synaptosomal fractions of six regions of the feline brain. The order of potency of 'binding' of both glutamate and aspartate to synaptosomal fractions of brain regions was: cerebellar cortex greater than caudate nucleus greater than or equal to cerebral cortex greater than medulla approximately pons greater than corona radiata. Glutamate was bound to a greater extent than aspartate to particles of all regions studied, except for cerebral cortex.", "PMID": 955005}
{"id": "pubmed23n0032_1459", "title": "Deficiency in renomedullary prostaglandin synthesis related to the evolution of essential hypertension.", "content": "Continued PG synthesis in the early stages of essential hypertension might reflect an activation of the renal antihypertensive function in respect to neurogenic and/or hormonal pression stimuli, subsequently a deficiency of renal PG synthesis related to irreversible changes with the kidney would lead to the prepoderance of a pressure mechanism, resulting to a further increase of blood pressure.", "contents": "Deficiency in renomedullary prostaglandin synthesis related to the evolution of essential hypertension. Continued PG synthesis in the early stages of essential hypertension might reflect an activation of the renal antihypertensive function in respect to neurogenic and/or hormonal pression stimuli, subsequently a deficiency of renal PG synthesis related to irreversible changes with the kidney would lead to the prepoderance of a pressure mechanism, resulting to a further increase of blood pressure.", "PMID": 955006}
{"id": "pubmed23n0032_1460", "title": "The Fusus coli of the rabbit as a pacemaker area.", "content": "Section of the rabbit's proximal colon orad to the fusus coli resulted in a marked reduction in both slow-wave frequency and differentiation of the two kinds of faeces produced. This indicates the existence of a pacemaker area and a mechanical role for the reversed oral-aboral gradient of the proximal colon.", "contents": "The Fusus coli of the rabbit as a pacemaker area. Section of the rabbit's proximal colon orad to the fusus coli resulted in a marked reduction in both slow-wave frequency and differentiation of the two kinds of faeces produced. This indicates the existence of a pacemaker area and a mechanical role for the reversed oral-aboral gradient of the proximal colon.", "PMID": 955009}
{"id": "pubmed23n0032_1461", "title": "High altitude influence on the level and turn-over time of cardiac norepinephrine in rats.", "content": "In rats exposed to an altitude of 2,900 meters the level of endogenous cardiac norepinephrine decreases after the 5th day of exposure. The turner-over time of the amine proceeds in 3 steps which are probably related to periods of stress and adaptation: initial increase, important decrease from the 5th day and return to a normal value from the 12th day.", "contents": "High altitude influence on the level and turn-over time of cardiac norepinephrine in rats. In rats exposed to an altitude of 2,900 meters the level of endogenous cardiac norepinephrine decreases after the 5th day of exposure. The turner-over time of the amine proceeds in 3 steps which are probably related to periods of stress and adaptation: initial increase, important decrease from the 5th day and return to a normal value from the 12th day.", "PMID": 955010}
{"id": "pubmed23n0032_1462", "title": "A new quinoline-carbamate aphicide.", "content": "We found that it was nearly impossible to apply the quinoline-carbamate aphicide per os by means of synthetic diets, owing to its high feeding-deterrent-effect. After application via the roots of the host plant, this systemic compound is deposited on the leaf surface. The results suggest that the toxic effect is not the result of the oral uptake of phloem sap, but of the tarsal contact with the toxicant. Sensitivity of aphids to this compound and LD50-values were determined after topical applications.", "contents": "A new quinoline-carbamate aphicide. We found that it was nearly impossible to apply the quinoline-carbamate aphicide per os by means of synthetic diets, owing to its high feeding-deterrent-effect. After application via the roots of the host plant, this systemic compound is deposited on the leaf surface. The results suggest that the toxic effect is not the result of the oral uptake of phloem sap, but of the tarsal contact with the toxicant. Sensitivity of aphids to this compound and LD50-values were determined after topical applications.", "PMID": 955011}
{"id": "pubmed23n0032_1463", "title": "3-hydroxy-3-methylglutaric acid and experimental atherosclerosis in rats.", "content": "In rats 3-hydroxy-3-methylglutaric acid effectively counteracts the lipemic and atherosclerotic response of massive doses of vitamin D2. It regressed the formation of atheromatous arterial lesions. Furthermore the significant decrease in serum beta-lipoprotein levels on HMG treatment could be due to decrease in VLDL triglyceride and cholesterol levels.", "contents": "3-hydroxy-3-methylglutaric acid and experimental atherosclerosis in rats. In rats 3-hydroxy-3-methylglutaric acid effectively counteracts the lipemic and atherosclerotic response of massive doses of vitamin D2. It regressed the formation of atheromatous arterial lesions. Furthermore the significant decrease in serum beta-lipoprotein levels on HMG treatment could be due to decrease in VLDL triglyceride and cholesterol levels.", "PMID": 955013}
{"id": "pubmed23n0032_1464", "title": "Effect of acetylcholine on melanophores of Rana tigrina.", "content": "Acetylcholine produced melanin aggregation and blanching of skin colour in Rana tigrina, the common Indian frog. The effects were more prolonged in frogs pretreated with an anticholinesterase agent. Acetylcholine effects were not antagonized by either m-cholinolytic (atropine) or n-cholinolytic (pentolinium) agents, but were markedly inhibited by procaine. The results have been discussed in the light of the well-known membrane-stabilizing effect of procaine.", "contents": "Effect of acetylcholine on melanophores of Rana tigrina. Acetylcholine produced melanin aggregation and blanching of skin colour in Rana tigrina, the common Indian frog. The effects were more prolonged in frogs pretreated with an anticholinesterase agent. Acetylcholine effects were not antagonized by either m-cholinolytic (atropine) or n-cholinolytic (pentolinium) agents, but were markedly inhibited by procaine. The results have been discussed in the light of the well-known membrane-stabilizing effect of procaine.", "PMID": 955014}
{"id": "pubmed23n0032_1465", "title": "Action of stannous and stannic chlorides on bacteria.", "content": "Stannous or stannic chlorides reduced the growth rate of K. aerogenes, Ps. reptilovora and an unidentified bacterium in a minimal liquid medium and on agar plates. The greatest effect was observed with K. aerogenes and was accompanied by a decreased viability, but 100% survival occurred with other strains. The metal was loosely bound to the cells and there was no direct correlation between the amount adsorbed and the biological response.", "contents": "Action of stannous and stannic chlorides on bacteria. Stannous or stannic chlorides reduced the growth rate of K. aerogenes, Ps. reptilovora and an unidentified bacterium in a minimal liquid medium and on agar plates. The greatest effect was observed with K. aerogenes and was accompanied by a decreased viability, but 100% survival occurred with other strains. The metal was loosely bound to the cells and there was no direct correlation between the amount adsorbed and the biological response.", "PMID": 955015}
{"id": "pubmed23n0032_1466", "title": "Nerves and muscles regulating feather follicle movements in the chicken.", "content": "It was found that most feather follicles posses more than a single muscle system but only single nerve endings were observed in a feather follicle. It seems that muscles exist which antagonize each other. Anastomoses of nerves originating from different sources and flowing of mixed nerves into the feather follicle could explain the antagonistic action of the muscles on the feather follicle.", "contents": "Nerves and muscles regulating feather follicle movements in the chicken. It was found that most feather follicles posses more than a single muscle system but only single nerve endings were observed in a feather follicle. It seems that muscles exist which antagonize each other. Anastomoses of nerves originating from different sources and flowing of mixed nerves into the feather follicle could explain the antagonistic action of the muscles on the feather follicle.", "PMID": 955016}
{"id": "pubmed23n0032_1467", "title": "DNA-mediated transformation in the platyfish-swordtail melanoma system.", "content": "A genetic marker, the tumor gene Tu, which causes the formation of abnormal melanophores, the T-melanophores, in the skin of Xiphophorine fish has been transferred by donor DNA from a Tu genotype to recipient embryos lacking Tu. Abnormal melanophores which are identical to the T-melanophores of the donor genotype occurred only in recipients treated with Tu-DNA and not in those treated with Tu-free control DNA.", "contents": "DNA-mediated transformation in the platyfish-swordtail melanoma system. A genetic marker, the tumor gene Tu, which causes the formation of abnormal melanophores, the T-melanophores, in the skin of Xiphophorine fish has been transferred by donor DNA from a Tu genotype to recipient embryos lacking Tu. Abnormal melanophores which are identical to the T-melanophores of the donor genotype occurred only in recipients treated with Tu-DNA and not in those treated with Tu-free control DNA.", "PMID": 955017}
{"id": "pubmed23n0032_1468", "title": "Reappearance in vivo of neuraminidase-sensitive sialic acid in L 5222 rat leukemia cells.", "content": "Cell electrophoretic data and quantitative sialic acid determination show that, 16 to 20 h after i.p. implantation of neuraminidase-treated L 5222 rat leukemia cells, the original sialic acid content at the cell periphery is reconstituted.", "contents": "Reappearance in vivo of neuraminidase-sensitive sialic acid in L 5222 rat leukemia cells. Cell electrophoretic data and quantitative sialic acid determination show that, 16 to 20 h after i.p. implantation of neuraminidase-treated L 5222 rat leukemia cells, the original sialic acid content at the cell periphery is reconstituted.", "PMID": 955018}
{"id": "pubmed23n0032_1469", "title": "Extravesicular noradrenaline in developing peripheral adrenergic nerves.", "content": "Using a fluorescence technique numerous developing noradrenergic nerve terminals were observed in the muscle coat of the rat ductus deferens between 2 and 6 days postpartum. In the electron microscope similar developing nerve terminals possessed an extensive system of tubular endoplasmic reticulum but did not contain the small dense cored vesicles characteristic of mature noradrenergic nerve terminals. Thus the tubular reticulum is proposed as an alternative storage site for noradrenaline in developing adrenergic nerves.", "contents": "Extravesicular noradrenaline in developing peripheral adrenergic nerves. Using a fluorescence technique numerous developing noradrenergic nerve terminals were observed in the muscle coat of the rat ductus deferens between 2 and 6 days postpartum. In the electron microscope similar developing nerve terminals possessed an extensive system of tubular endoplasmic reticulum but did not contain the small dense cored vesicles characteristic of mature noradrenergic nerve terminals. Thus the tubular reticulum is proposed as an alternative storage site for noradrenaline in developing adrenergic nerves.", "PMID": 955020}
{"id": "pubmed23n0032_1470", "title": "Plating efficiency of mouse embryo cells as a function of gestational age.", "content": "Mouse embryo cells can be plated directly from single-cell suspensions of fresh embryos. For randomly bred Swiss mice, there is an increase in plating efficiency as a function of gestational age. Colony-forming units appear at about day 10 and there is an exponential increase in colony-forming ability up to day 16, after which there is no further increase.", "contents": "Plating efficiency of mouse embryo cells as a function of gestational age. Mouse embryo cells can be plated directly from single-cell suspensions of fresh embryos. For randomly bred Swiss mice, there is an increase in plating efficiency as a function of gestational age. Colony-forming units appear at about day 10 and there is an exponential increase in colony-forming ability up to day 16, after which there is no further increase.", "PMID": 955021}
{"id": "pubmed23n0032_1471", "title": "Kinetics of DNA repair synthesis in guinea-pig pancreatic slices following in vitro exposure to N-methyl-N-nitrosourethane.", "content": "In vitro exposure of guinea-pig pancreatic slices to NMUT resulted in an increase in hydroxyurea-insensitive 3H-TdR incorporation into DNA; this represents DNA repair synthesis following NMUT-induced DNA damage. The kinetics of this hydroxyurea-insensitive 3H-TdR incorporation suggest that the NMUT-induced DNA damage is largely repaired within 2 hours.", "contents": "Kinetics of DNA repair synthesis in guinea-pig pancreatic slices following in vitro exposure to N-methyl-N-nitrosourethane. In vitro exposure of guinea-pig pancreatic slices to NMUT resulted in an increase in hydroxyurea-insensitive 3H-TdR incorporation into DNA; this represents DNA repair synthesis following NMUT-induced DNA damage. The kinetics of this hydroxyurea-insensitive 3H-TdR incorporation suggest that the NMUT-induced DNA damage is largely repaired within 2 hours.", "PMID": 955022}
{"id": "pubmed23n0032_1472", "title": "Peripheral sympathetic innervation of the deep pineal gland of the golden hamster.", "content": "Both the superficial and deep pineal components of the intact hamster contain a rich network of green to yellow-green fluorescent nerve fibres. After either superior cervical ganglionectomy or after transection of the nervi conarii the majority of the fluorescing fibres disappeared from both the superficial and deep pineal masses. Although the deep pineal remained intact after surgical removal of the superficial pineal, it was devoid of any green or yellow-green fluorescent fibres.", "contents": "Peripheral sympathetic innervation of the deep pineal gland of the golden hamster. Both the superficial and deep pineal components of the intact hamster contain a rich network of green to yellow-green fluorescent nerve fibres. After either superior cervical ganglionectomy or after transection of the nervi conarii the majority of the fluorescing fibres disappeared from both the superficial and deep pineal masses. Although the deep pineal remained intact after surgical removal of the superficial pineal, it was devoid of any green or yellow-green fluorescent fibres.", "PMID": 955024}
{"id": "pubmed23n0032_1473", "title": "[Development of the rudimentary prostates of Ellobius lutescens (Microtinae) in organ culture; effects of androgens (author's transl)].", "content": "The structure of the rudimentary prostates of Ellobius lutescens is maintained intact after 6 days of organotypic culture in the absence of male hormones. Comparison with controls even shows a noticeable increase in the size of the epithelial cells. Adding male hormones to the culture medium does not modify the morphology of adult prostates, while it induces a sharp stimulation of immature prostates. In accordance with our previous results, these experiments show that the prostates of Ellobius lutescens lose their sensivity to androgens after puberty.", "contents": "[Development of the rudimentary prostates of Ellobius lutescens (Microtinae) in organ culture; effects of androgens (author's transl)]. The structure of the rudimentary prostates of Ellobius lutescens is maintained intact after 6 days of organotypic culture in the absence of male hormones. Comparison with controls even shows a noticeable increase in the size of the epithelial cells. Adding male hormones to the culture medium does not modify the morphology of adult prostates, while it induces a sharp stimulation of immature prostates. In accordance with our previous results, these experiments show that the prostates of Ellobius lutescens lose their sensivity to androgens after puberty.", "PMID": 955025}
{"id": "pubmed23n0032_1474", "title": "Large scale cultivation of a free-living nematode (Caenorhabditis elegans).", "content": "A method is presented for the large scale cultivation of the free-living nematode Caenorhabditis elegans, using continuous aeration and agitation in glass ware (stirrer flasks) developed for the continuous culture of suspended cells. With this technique, populations up to 10(9) nematodes may be obtained in a 10 1 culture in less than 6 weeks with an inoculum of some 50 worms. Costs can be reduced by using an inexpensive yeast extract, available from the food industry.", "contents": "Large scale cultivation of a free-living nematode (Caenorhabditis elegans). A method is presented for the large scale cultivation of the free-living nematode Caenorhabditis elegans, using continuous aeration and agitation in glass ware (stirrer flasks) developed for the continuous culture of suspended cells. With this technique, populations up to 10(9) nematodes may be obtained in a 10 1 culture in less than 6 weeks with an inoculum of some 50 worms. Costs can be reduced by using an inexpensive yeast extract, available from the food industry.", "PMID": 955027}
{"id": "pubmed23n0032_1475", "title": "Continuous labelling method for autoradiographic analysis of cell cycle parameters in steady state cell systems.", "content": "Under certain conditions, continuous availability of 3H-thymidine in steady-state cell systems results in a linear increase of the fraction of labelled cells, the equation of which can be used to determine cell cycle parameters of the system investigated.", "contents": "Continuous labelling method for autoradiographic analysis of cell cycle parameters in steady state cell systems. Under certain conditions, continuous availability of 3H-thymidine in steady-state cell systems results in a linear increase of the fraction of labelled cells, the equation of which can be used to determine cell cycle parameters of the system investigated.", "PMID": 955028}
{"id": "pubmed23n0032_1476", "title": "Phaeomelanic pigments from a human melanoma.", "content": "The pigments in melanomas from 2 patients were studied with regard to solubility and chemical composition. Melanoma pigment from a patient with red-blonde hair was alkali-soluble and contained 9 or 10% sulfur and was thus of phaeomelanic type. Melanoma pigment from a patient with red-brown hair was insoluble in 0.2 N NaOH. Its sulfur content was 6%. This pigment was eumelanic with regard to solubility characteristics but the sulfur content was higher than previously observed for eumelanin.", "contents": "Phaeomelanic pigments from a human melanoma. The pigments in melanomas from 2 patients were studied with regard to solubility and chemical composition. Melanoma pigment from a patient with red-blonde hair was alkali-soluble and contained 9 or 10% sulfur and was thus of phaeomelanic type. Melanoma pigment from a patient with red-brown hair was insoluble in 0.2 N NaOH. Its sulfur content was 6%. This pigment was eumelanic with regard to solubility characteristics but the sulfur content was higher than previously observed for eumelanin.", "PMID": 955030}
{"id": "pubmed23n0032_1477", "title": "Resistance of purified cholera toxin to enzymatic treatment with pancreatic elastase and papain.", "content": "Treatment of Cholera toxin with pancreatic elastase and papain in vitro showed a high resistance of the toxin molecule to these enzymes, under non-denaturing conditions or in the presence of 2 M urea. These experiments support the hypothesis of a particularly stable molecular structure of the toxin, as an explanation of its activity in the intestinal lumen where the pancreatic proteases are active.", "contents": "Resistance of purified cholera toxin to enzymatic treatment with pancreatic elastase and papain. Treatment of Cholera toxin with pancreatic elastase and papain in vitro showed a high resistance of the toxin molecule to these enzymes, under non-denaturing conditions or in the presence of 2 M urea. These experiments support the hypothesis of a particularly stable molecular structure of the toxin, as an explanation of its activity in the intestinal lumen where the pancreatic proteases are active.", "PMID": 955031}
{"id": "pubmed23n0032_1478", "title": "Genotype dependence of monoamine oxidase in inbred strains of mice.", "content": "MAO activity was found to be influenced by the genotype or strain of mouse up to 20 days of age. The strain differences observed may derive from different rates of brain development. A number of neurological mutations comprizing three pathological classes had no effect on MAO.", "contents": "Genotype dependence of monoamine oxidase in inbred strains of mice. MAO activity was found to be influenced by the genotype or strain of mouse up to 20 days of age. The strain differences observed may derive from different rates of brain development. A number of neurological mutations comprizing three pathological classes had no effect on MAO.", "PMID": 955032}
{"id": "pubmed23n0032_1479", "title": "Serum enzyme activities in the African elephant (loxodonta africana).", "content": "The serum activities of aspartate aminotransferase, alanine aminotransferase, alpha-hydroxybutyrate dehydrogenase and creatine phosphokinase have been measured in the African elephant. In general, the values were broadly comparable with those of man except that alanine aminotransferase was much lower and creatine phosphokinase higher. No variation due to age, sex, season or location was observed.", "contents": "Serum enzyme activities in the African elephant (loxodonta africana). The serum activities of aspartate aminotransferase, alanine aminotransferase, alpha-hydroxybutyrate dehydrogenase and creatine phosphokinase have been measured in the African elephant. In general, the values were broadly comparable with those of man except that alanine aminotransferase was much lower and creatine phosphokinase higher. No variation due to age, sex, season or location was observed.", "PMID": 955033}
{"id": "pubmed23n0032_1480", "title": "Labile protein-methyl ester: comparison between chemically and enzymatically synthesized.", "content": "The rate of hydrolysis of protein-methyl ester, the enzymatic product of S-adenosylmethionine: protein-carboxyl methyltransferase (EC.2.1.1.24) acting on oxidized ribonuclease, was measured at pH 7.1 and 8.6 at 37 degrees C. The half-life of the hydrolysis of the ester is 25 min at pH 7.1, and 4 min at 8.6. The rate of hydrolysis of the enzymatically formed esters at pH 7.0, in 0.1 M phosphate buffer, was about 25 times faster than that of esters formed chemically by reaction with methanol in HCl. The lability of the enzymatically synthesized protein-methyl ester suggests that the esterification is specific to sites such that ionization of neighboring amino acid side chains enhances the rate of the hydrolysis.", "contents": "Labile protein-methyl ester: comparison between chemically and enzymatically synthesized. The rate of hydrolysis of protein-methyl ester, the enzymatic product of S-adenosylmethionine: protein-carboxyl methyltransferase (EC.2.1.1.24) acting on oxidized ribonuclease, was measured at pH 7.1 and 8.6 at 37 degrees C. The half-life of the hydrolysis of the ester is 25 min at pH 7.1, and 4 min at 8.6. The rate of hydrolysis of the enzymatically formed esters at pH 7.0, in 0.1 M phosphate buffer, was about 25 times faster than that of esters formed chemically by reaction with methanol in HCl. The lability of the enzymatically synthesized protein-methyl ester suggests that the esterification is specific to sites such that ionization of neighboring amino acid side chains enhances the rate of the hydrolysis.", "PMID": 955034}
{"id": "pubmed23n0032_1481", "title": "Early development of gap junctions between the mouse embryonic myocardial cells. A freeze-etching study.", "content": "Using the freeze-etch technique, nexuses have been shown to exist at a very early stage in developing mouse hearts (10 dpc). At this time they are rare, but become more progressively frequent and extensive at 12 and 14 dpc. Special arrangements of particles progressively observed on the fracture faces PF (linear arrays, small associated groups of linear arrays, then hexagonal arrays with 'arms' formed by linear clusters) suggest that in ontogenesis the gap junctions may be built up by successive aggregation of the linear arrays of particles.", "contents": "Early development of gap junctions between the mouse embryonic myocardial cells. A freeze-etching study. Using the freeze-etch technique, nexuses have been shown to exist at a very early stage in developing mouse hearts (10 dpc). At this time they are rare, but become more progressively frequent and extensive at 12 and 14 dpc. Special arrangements of particles progressively observed on the fracture faces PF (linear arrays, small associated groups of linear arrays, then hexagonal arrays with 'arms' formed by linear clusters) suggest that in ontogenesis the gap junctions may be built up by successive aggregation of the linear arrays of particles.", "PMID": 955039}
{"id": "pubmed23n0032_1482", "title": "Synthesis of new alpha-hydrazinoarylacetic acids and derivatives.", "content": "The synthesis of some alpha-hydrazinoarylacetic acids (I) by reaction of alpha-bromoarylacetic acids with hydrazine, alkylhydrazines and carbobenzyloxyhydrazines is described. Reduction of the hydrazones of 2- and 3-thienylglyoxylic acids provided a general and effective route to the thienylic series. In view of the use of compounds (I) for the preparation of new penicillins, the experimental conditions for their conversion into cyclohexylamides (XI) via the corresponding carbobenzyloxyderivatives (III) were also investigated.", "contents": "Synthesis of new alpha-hydrazinoarylacetic acids and derivatives. The synthesis of some alpha-hydrazinoarylacetic acids (I) by reaction of alpha-bromoarylacetic acids with hydrazine, alkylhydrazines and carbobenzyloxyhydrazines is described. Reduction of the hydrazones of 2- and 3-thienylglyoxylic acids provided a general and effective route to the thienylic series. In view of the use of compounds (I) for the preparation of new penicillins, the experimental conditions for their conversion into cyclohexylamides (XI) via the corresponding carbobenzyloxyderivatives (III) were also investigated.", "PMID": 955057}
{"id": "pubmed23n0032_1483", "title": "Circular dichroism studies of some aralkylamines.", "content": "Circular dichroism curves are reported for a number of aralkylamines having a general formula R--CH(NH2)--R' (in which R and R' are alkyl-, aryl- or aralkyl-groups) and for 1-aminobenzocyclobutene, 1-aminoindane, 1-amino-1,2,3,4-tetrahydronaphthalene and 2-amino-1,2,3,4-tetrahydronaphthalene. The Cotton effects due to aromatic chromophore 1Lb and 1La absorption bands are discussed and simple correlations between absolute configuration and the signs of Cotton effects are deduced.", "contents": "Circular dichroism studies of some aralkylamines. Circular dichroism curves are reported for a number of aralkylamines having a general formula R--CH(NH2)--R' (in which R and R' are alkyl-, aryl- or aralkyl-groups) and for 1-aminobenzocyclobutene, 1-aminoindane, 1-amino-1,2,3,4-tetrahydronaphthalene and 2-amino-1,2,3,4-tetrahydronaphthalene. The Cotton effects due to aromatic chromophore 1Lb and 1La absorption bands are discussed and simple correlations between absolute configuration and the signs of Cotton effects are deduced.", "PMID": 955058}
{"id": "pubmed23n0032_1484", "title": "Synthesis and pharmacological activity of some 3-amino-11H-indolo[3,2-c] [1,8]naphthyridines.", "content": "The preparation of some 3-amino-11H-indolo [3,2-c]-[1,8] naphthyridines using the Fischer indole synthesis on the appropriate phenylhydrazones is described. Some compounds (IV b, c, d) were effective in inhibiting  the reactions of delayed hypersensitivity, but the testing has been discontinued because of toxicity observed.", "contents": "Synthesis and pharmacological activity of some 3-amino-11H-indolo[3,2-c] [1,8]naphthyridines. The preparation of some 3-amino-11H-indolo [3,2-c]-[1,8] naphthyridines using the Fischer indole synthesis on the appropriate phenylhydrazones is described. Some compounds (IV b, c, d) were effective in inhibiting  the reactions of delayed hypersensitivity, but the testing has been discontinued because of toxicity observed.", "PMID": 955059}
{"id": "pubmed23n0032_1485", "title": "Structure-activity relationships for homoserine derivatives as inhibitors of the enzymatic synthesis of S-adenosyl-L-methionine.", "content": "The adenosyltransferase inhibitory activities of a series of homoserine derivatives were studied using Hansch equations and CNDO/2 calculations. Structure-activity relationships are established involving electronic and lipophilic parameters.", "contents": "Structure-activity relationships for homoserine derivatives as inhibitors of the enzymatic synthesis of S-adenosyl-L-methionine. The adenosyltransferase inhibitory activities of a series of homoserine derivatives were studied using Hansch equations and CNDO/2 calculations. Structure-activity relationships are established involving electronic and lipophilic parameters.", "PMID": 955060}
{"id": "pubmed23n0032_1486", "title": "[Research on substances with antiviral activity. V. Structural analogs of arginine].", "content": "Some arginine analogues were synthesized and tested for antiviral activity. Two compounds showed weak activity in vitro against vaccinia virus.", "contents": "[Research on substances with antiviral activity. V. Structural analogs of arginine]. Some arginine analogues were synthesized and tested for antiviral activity. Two compounds showed weak activity in vitro against vaccinia virus.", "PMID": 955061}
{"id": "pubmed23n0032_1487", "title": "[\"De novo substituent constants\" in the study of quantitative structure-activity relationship].", "content": "The basic features of the Free-Wilson method for assigning additivity constants to structural features of related compounds is described. The method is presented in considerable operational detail with special emphasis on its development. An original example is discussed. Showing that the substituent  constants can be related to more fundamental physico-chemical substituent parameters such as the hydrophobic constants pi.", "contents": "[\"De novo substituent constants\" in the study of quantitative structure-activity relationship]. The basic features of the Free-Wilson method for assigning additivity constants to structural features of related compounds is described. The method is presented in considerable operational detail with special emphasis on its development. An original example is discussed. Showing that the substituent  constants can be related to more fundamental physico-chemical substituent parameters such as the hydrophobic constants pi.", "PMID": 955062}
{"id": "pubmed23n0032_1488", "title": "A simple method for the perfusion of isolated liver cells.", "content": "The perfusion technique described in this paper offers a new and simple method for studying liver cell metabolism in a more dynamic way. It has potential use in hormonal response studies, pulsechase experiments and many other types of investigations. Finally, the perfusion technique may be applicable to other isolated cell preparations as well. The method combines some advantages of the flow-through technique with the great advantages of using isolated liver cells, apparently without disturbing the viability of the cells.", "contents": "A simple method for the perfusion of isolated liver cells. The perfusion technique described in this paper offers a new and simple method for studying liver cell metabolism in a more dynamic way. It has potential use in hormonal response studies, pulsechase experiments and many other types of investigations. Finally, the perfusion technique may be applicable to other isolated cell preparations as well. The method combines some advantages of the flow-through technique with the great advantages of using isolated liver cells, apparently without disturbing the viability of the cells.", "PMID": 955103}
{"id": "pubmed23n0032_1489", "title": "The victim is guilty.", "content": "Our society in many situations tends to blame the victim. Questions are raised whether this occurs more commonly with women and what its effects are. An examination of the treatment of women as patients may help answer these questions. In 1971 twice as many men as women were on chronic hemodialysis, raising the possibility of different allotment of medical resources and different admission criterion for this very expensive therapy. This example only indicates that women may be treated differently. The following commentary indicates women may be blamed for their illness. A prestigious medical journal has published articles claiming the clinical syndrome, Iceland disease, is really epidemic hysteria. One of the three main reasons given for such a claim is that it occurs primarily in women. Aside from the fact that many physiologic diseases do have skewed sex ratios, one wonders why it is considered inappropriate for a physiologic disease to have a male/femal ratio different from 1.0 yet not so for a psychological disease. Once women are put into this category, in essence blamed for their illness, there will be no more search for pathology that might be corrected. they may undergo detrimental therapy, in this case removal of their uteri-a hysterectomy, probably in the true sense of the word.", "contents": "The victim is guilty. Our society in many situations tends to blame the victim. Questions are raised whether this occurs more commonly with women and what its effects are. An examination of the treatment of women as patients may help answer these questions. In 1971 twice as many men as women were on chronic hemodialysis, raising the possibility of different allotment of medical resources and different admission criterion for this very expensive therapy. This example only indicates that women may be treated differently. The following commentary indicates women may be blamed for their illness. A prestigious medical journal has published articles claiming the clinical syndrome, Iceland disease, is really epidemic hysteria. One of the three main reasons given for such a claim is that it occurs primarily in women. Aside from the fact that many physiologic diseases do have skewed sex ratios, one wonders why it is considered inappropriate for a physiologic disease to have a male/femal ratio different from 1.0 yet not so for a psychological disease. Once women are put into this category, in essence blamed for their illness, there will be no more search for pathology that might be corrected. they may undergo detrimental therapy, in this case removal of their uteri-a hysterectomy, probably in the true sense of the word.", "PMID": 955123}
{"id": "pubmed23n0032_1490", "title": "Adventures of a woman in science.", "content": "This paper is divided into two parts. The first part describes the difficulties encountered in entering the scientific profession and staying there if one is a woman; the difficulties in combating the degrading sterotypes about women that are commonly held (women are believed to be submissive, unintelligent, uncreative, etc.); and the discrimination that accompanies beliefs in these sterotypes. The second part of the paper examines the evidence for the truth or falsity of the proposition that these stereotypes reflect woman's \"innate\" nature, and shows that there is no evidence to suggest that the proposition is true. On the other hand, there is evidence from social psychology that, for the most part, people act the way people around them expect them to act, and they believe that they are what others expect them to be. So if the scientific profession holds the outragious and erroneous belief that women can't be scientists, it is likely that few women will become scientists. This must change: the integrity of the progession is at stake.", "contents": "Adventures of a woman in science. This paper is divided into two parts. The first part describes the difficulties encountered in entering the scientific profession and staying there if one is a woman; the difficulties in combating the degrading sterotypes about women that are commonly held (women are believed to be submissive, unintelligent, uncreative, etc.); and the discrimination that accompanies beliefs in these sterotypes. The second part of the paper examines the evidence for the truth or falsity of the proposition that these stereotypes reflect woman's \"innate\" nature, and shows that there is no evidence to suggest that the proposition is true. On the other hand, there is evidence from social psychology that, for the most part, people act the way people around them expect them to act, and they believe that they are what others expect them to be. So if the scientific profession holds the outragious and erroneous belief that women can't be scientists, it is likely that few women will become scientists. This must change: the integrity of the progession is at stake.", "PMID": 955124}
{"id": "pubmed23n0032_1491", "title": "Women and discrimination in academe.", "content": "Affirmative action on the campus has led to some changes, but women and minorities have made little headway, especially in terms of being hired. At some institutions, after a year or two of affirmative action, the number and percentage of women has decreased while the number and percentage of men increased. The greatest progress has been in the areas of new legislation, general awareness, and correction of salary inequities. Various laws and court decisions relative to discrimination are discussed, as well as the relationship between women's and minority issues--Sandler, B. Women and discrimination in academe.", "contents": "Women and discrimination in academe. Affirmative action on the campus has led to some changes, but women and minorities have made little headway, especially in terms of being hired. At some institutions, after a year or two of affirmative action, the number and percentage of women has decreased while the number and percentage of men increased. The greatest progress has been in the areas of new legislation, general awareness, and correction of salary inequities. Various laws and court decisions relative to discrimination are discussed, as well as the relationship between women's and minority issues--Sandler, B. Women and discrimination in academe.", "PMID": 955125}
{"id": "pubmed23n0032_1492", "title": "Genetic influence on nutritional aspects of metabolic regulation I.", "content": "Examples of two genetic models of growth and development are described. Mice selected for growth rate from 3 to 6 weeks of age show an increase in mature body size and an increase in tissue cellularity. Manipulation of dietary intake at various ages resulted in different responses in the control and growth strain mice. Futher trials are needed to determine metabolic or endocrine adaptations that are responsible for enhanced growth in these mice. The second model discussed was the genetically obese Zucker rat. When compared to its lean littermates, the obese rat is hyperphagic and metabolically efficient in storing calories as fat, and has impaired development of muscle tissue. The shift in the growth of muscle tissue to the growth of adipose tissue cannot be prevented by dietary manipulation or exercise. Increases in liver lipogenic rate can be prevented by feeding a high fat diet. It appears from the nutritional studies presented hear that many of the metabolic lesions associated with the development of spontaneous obesity in this animal model are secondary to the primary causal event.", "contents": "Genetic influence on nutritional aspects of metabolic regulation I. Examples of two genetic models of growth and development are described. Mice selected for growth rate from 3 to 6 weeks of age show an increase in mature body size and an increase in tissue cellularity. Manipulation of dietary intake at various ages resulted in different responses in the control and growth strain mice. Futher trials are needed to determine metabolic or endocrine adaptations that are responsible for enhanced growth in these mice. The second model discussed was the genetically obese Zucker rat. When compared to its lean littermates, the obese rat is hyperphagic and metabolically efficient in storing calories as fat, and has impaired development of muscle tissue. The shift in the growth of muscle tissue to the growth of adipose tissue cannot be prevented by dietary manipulation or exercise. Increases in liver lipogenic rate can be prevented by feeding a high fat diet. It appears from the nutritional studies presented hear that many of the metabolic lesions associated with the development of spontaneous obesity in this animal model are secondary to the primary causal event.", "PMID": 955126}
{"id": "pubmed23n0032_1493", "title": "Inadequate cervical mucus--a cause of \"idiopathic\" infertility.", "content": "The purpose of this study was to investigate and treat a group of patients referred for \"idiopathic\" infertility in whom no apparent cause for infertility, apart from inadequate cervical mucus, was found. Hormone investigations revealed that these patients could be divided into two groups: those with low sex steroid profiles despite apparent ovulation, and a second group with normal sex steroid profiles. All patients were treated with ovulation-inducing agents in an attempt to produce \"controlled\" ovarian hyperstimulation and an improved cervical mucus. Four of six patients conceived. The rationale behind the use of ovulation-inducing agents in this situation is discussed.", "contents": "Inadequate cervical mucus--a cause of \"idiopathic\" infertility. The purpose of this study was to investigate and treat a group of patients referred for \"idiopathic\" infertility in whom no apparent cause for infertility, apart from inadequate cervical mucus, was found. Hormone investigations revealed that these patients could be divided into two groups: those with low sex steroid profiles despite apparent ovulation, and a second group with normal sex steroid profiles. All patients were treated with ovulation-inducing agents in an attempt to produce \"controlled\" ovarian hyperstimulation and an improved cervical mucus. Four of six patients conceived. The rationale behind the use of ovulation-inducing agents in this situation is discussed.", "PMID": 955130}
{"id": "pubmed23n0032_1494", "title": "Studies on low-dose oral contraceptives: cervical mucus and plasma hormone changes in relation to circulating D-norgestrel and 17alpha ethynyl-estradiol concentrations.", "content": "A study was undertaken to determine the effects of a low-dose oral contraceptive comprising 150 mug of D-norgestrel and 30 mug of 17alpha-ethynyl estradiol (Microgynon) on the plasma levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17BETA-ESTRADIOL, AND PROGESTERONE AND ON THE PHYSICAl properties of cervical mucus. Samples of blood and cervical mucus were obtained from three women during a treated cycle and the immediately-following \"withdrawal\" cycle. Specific radioimmunoassay methods were used to determine LH, FSH, 17beta-estradiol, and progesterone levels in treated and withdrawal cycles, and D-norgestrel and 17alpha-ethynyl estradiol in samples obtained during treated cycles. The concentration of synthetic steroids was also measured in blood samples obtained before and 1 hour after ingestion of the contraceptive to determine the maximal daily variation. The results indicated that the contraceptive action of this combined low-dose oral contraceptive is mediated through suppression of ovulation and by rendering the cervical mucus impenetrable to sperm. Plasma FSH levels appeared to be one of the most sensitive indices of suppression. Determination of D-norgestrel and 17alpha-ethynyl estradiol showed that 3 to 4 days were required to reach maximal plasma levels and that daily fluctuations were considerable. Withdrawal of the pill resulted in an immediate return to ovulatory cycles in all three subjects studied.", "contents": "Studies on low-dose oral contraceptives: cervical mucus and plasma hormone changes in relation to circulating D-norgestrel and 17alpha ethynyl-estradiol concentrations. A study was undertaken to determine the effects of a low-dose oral contraceptive comprising 150 mug of D-norgestrel and 30 mug of 17alpha-ethynyl estradiol (Microgynon) on the plasma levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17BETA-ESTRADIOL, AND PROGESTERONE AND ON THE PHYSICAl properties of cervical mucus. Samples of blood and cervical mucus were obtained from three women during a treated cycle and the immediately-following \"withdrawal\" cycle. Specific radioimmunoassay methods were used to determine LH, FSH, 17beta-estradiol, and progesterone levels in treated and withdrawal cycles, and D-norgestrel and 17alpha-ethynyl estradiol in samples obtained during treated cycles. The concentration of synthetic steroids was also measured in blood samples obtained before and 1 hour after ingestion of the contraceptive to determine the maximal daily variation. The results indicated that the contraceptive action of this combined low-dose oral contraceptive is mediated through suppression of ovulation and by rendering the cervical mucus impenetrable to sperm. Plasma FSH levels appeared to be one of the most sensitive indices of suppression. Determination of D-norgestrel and 17alpha-ethynyl estradiol showed that 3 to 4 days were required to reach maximal plasma levels and that daily fluctuations were considerable. Withdrawal of the pill resulted in an immediate return to ovulatory cycles in all three subjects studied.", "PMID": 955131}
{"id": "pubmed23n0032_1495", "title": "Carbohydrate and lipid metabolic studies before and after one year of treatment with ethynodiol diacetate in \"normal\" women.", "content": "A 3-hour oral glucose tolerance test was conducted in \"normal\" women before and after 12 months of contraceptive treatment with daily 0.25-mg ethynodiol diacetate tablets. There was no significant change in the subjects' weight between the two tests. All but the 3-hour individual group glucose values were significantly elevated at the 1-year test, and 16.7% of the tolerance curves became \"borderline abnormal\". The group plasma insulin values for the 0.5-, 1-, and 2-hour samples during the 1-year test were also significantly elevated. There was a significant decrease in the group fasting triglyceride levels but no change in the fasting cholesterol levels. These data suggest that some of the 19-norprogestogens may adversely affect carbohydrate metabolism.", "contents": "Carbohydrate and lipid metabolic studies before and after one year of treatment with ethynodiol diacetate in \"normal\" women. A 3-hour oral glucose tolerance test was conducted in \"normal\" women before and after 12 months of contraceptive treatment with daily 0.25-mg ethynodiol diacetate tablets. There was no significant change in the subjects' weight between the two tests. All but the 3-hour individual group glucose values were significantly elevated at the 1-year test, and 16.7% of the tolerance curves became \"borderline abnormal\". The group plasma insulin values for the 0.5-, 1-, and 2-hour samples during the 1-year test were also significantly elevated. There was a significant decrease in the group fasting triglyceride levels but no change in the fasting cholesterol levels. These data suggest that some of the 19-norprogestogens may adversely affect carbohydrate metabolism.", "PMID": 955132}
{"id": "pubmed23n0032_1496", "title": "Histology of the endometrium in long-term use of a sequential oral contraceptive.", "content": "Non-atypical adenomatous hyperplasia was found in 13.5% of 111 biopsies obtained from long-term, predominantly black, users of Oracon. An association between length of time of use (95 cycles for those with adenomatous hyperplasia versus 73 for those without) and the development of adenomatous hyperplasia was noted, but there was no association with hypertension or obesity. Cystic glandular dilatation was seen in 63% but was not thought to represent hyperplasia. No more advanced lesions were seen.", "contents": "Histology of the endometrium in long-term use of a sequential oral contraceptive. Non-atypical adenomatous hyperplasia was found in 13.5% of 111 biopsies obtained from long-term, predominantly black, users of Oracon. An association between length of time of use (95 cycles for those with adenomatous hyperplasia versus 73 for those without) and the development of adenomatous hyperplasia was noted, but there was no association with hypertension or obesity. Cystic glandular dilatation was seen in 63% but was not thought to represent hyperplasia. No more advanced lesions were seen.", "PMID": 955133}
{"id": "pubmed23n0032_1497", "title": "17beta-ol androgens and free index in hirsute and hirsute obese women.", "content": "We have found the mean levels of combined serum testosterone and dihydrotestosterone (T+DHT) and the free index (FI) to be significantly higher and the mean dihydrotestosterone precipitation index (DHT-PI) to be significantly lower in hirsute women than in normal women. Although the mean T+DHT values of the different groups of hirsute patients were comparable, the FI value of the oligomenorrheic and/or obese patient was higher than that of the nonobese, normally menstruating group. In addition, the mean DHT-PI level of obese patients was significantly lower than that of nonobese patients. The lowest androgen binding was found in obese patients with oligomenorrhea. In our experience, hirsutism is associated with T+DHT values of 150 ng/dl or lower. Measurement of androgen binding and androgen levels in unchromatographed serum extracts provides valuable information in the treatment of hirsute women.", "contents": "17beta-ol androgens and free index in hirsute and hirsute obese women. We have found the mean levels of combined serum testosterone and dihydrotestosterone (T+DHT) and the free index (FI) to be significantly higher and the mean dihydrotestosterone precipitation index (DHT-PI) to be significantly lower in hirsute women than in normal women. Although the mean T+DHT values of the different groups of hirsute patients were comparable, the FI value of the oligomenorrheic and/or obese patient was higher than that of the nonobese, normally menstruating group. In addition, the mean DHT-PI level of obese patients was significantly lower than that of nonobese patients. The lowest androgen binding was found in obese patients with oligomenorrhea. In our experience, hirsutism is associated with T+DHT values of 150 ng/dl or lower. Measurement of androgen binding and androgen levels in unchromatographed serum extracts provides valuable information in the treatment of hirsute women.", "PMID": 955134}
{"id": "pubmed23n0032_1498", "title": "Levels of luteinizing hormone in semen of fertile and infertile men and possible significance of luteinizing hormone in sperm metabolism.", "content": "Luteinizing hormone (LH) levels were measured in the seminal plasma of 68 fertile and infertile men. LH levels in the seminal plasma were severalfold higher than those normally found in serum and were significantly higher in oligospermic and normospermic samples than in azoospermic samples. However, no significant difference was observed in LH levels of oligospermic and normospermic men. The effects of LH on fructose utilization, glucose oxidation, and adenyl cyclase activity of spermatozoa were also examined. The results indicate a possible role of seminal plasma LH in sperm motility and metabolism.", "contents": "Levels of luteinizing hormone in semen of fertile and infertile men and possible significance of luteinizing hormone in sperm metabolism. Luteinizing hormone (LH) levels were measured in the seminal plasma of 68 fertile and infertile men. LH levels in the seminal plasma were severalfold higher than those normally found in serum and were significantly higher in oligospermic and normospermic samples than in azoospermic samples. However, no significant difference was observed in LH levels of oligospermic and normospermic men. The effects of LH on fructose utilization, glucose oxidation, and adenyl cyclase activity of spermatozoa were also examined. The results indicate a possible role of seminal plasma LH in sperm motility and metabolism.", "PMID": 955135}
{"id": "pubmed23n0032_1499", "title": "Vasectomy and vasovasostomy. I. Timing of histologic changes in immature and mature dog testis after vasectomy.", "content": "The effects of vasectomy on the development and maintenance of spermatogenesis was studied using immature and mature dog testes. Bilateral vasectomy in immature dogs delayed the development of advanced spermatocytes, spermatids, and spermatozoa for about 3 months postsurgery. Spermatogenesis appeared to be recovered to control levels by 4 months postvasectomy. Spermatogenesis in mature dogs was also altered after bilateral vasectomy. Decreased numbers of advanced spermatocytes as well as maturation arrest was observed by 3 weeks postvasectomy. Seminiferous tubule cell layers quickly decreased to one to three layers as the lumina became occluded with sloughed cells by 3 to 6 weeks postvasectomy. Recovery in terms of the numbers of spermatocytes, spermatids, and spermatozoa was evident by 13 weeks postvasectomy, although occasional dog testes did not recover and appeared to be more sensitive to vasectomy-induced damage. It thus appears that vasectomy temporarily inhibits both the development and maintenance of spermatogenesis in immature as well as mature dog testes. Spermatogenesis does recover but may be maintained at somewhat lower levels after vasectomy. Changes are rapid in onset and take several weeks to be reversed. Some dog testes appear to be more sensitive to the damage and it may be irreversible in these testes.", "contents": "Vasectomy and vasovasostomy. I. Timing of histologic changes in immature and mature dog testis after vasectomy. The effects of vasectomy on the development and maintenance of spermatogenesis was studied using immature and mature dog testes. Bilateral vasectomy in immature dogs delayed the development of advanced spermatocytes, spermatids, and spermatozoa for about 3 months postsurgery. Spermatogenesis appeared to be recovered to control levels by 4 months postvasectomy. Spermatogenesis in mature dogs was also altered after bilateral vasectomy. Decreased numbers of advanced spermatocytes as well as maturation arrest was observed by 3 weeks postvasectomy. Seminiferous tubule cell layers quickly decreased to one to three layers as the lumina became occluded with sloughed cells by 3 to 6 weeks postvasectomy. Recovery in terms of the numbers of spermatocytes, spermatids, and spermatozoa was evident by 13 weeks postvasectomy, although occasional dog testes did not recover and appeared to be more sensitive to vasectomy-induced damage. It thus appears that vasectomy temporarily inhibits both the development and maintenance of spermatogenesis in immature as well as mature dog testes. Spermatogenesis does recover but may be maintained at somewhat lower levels after vasectomy. Changes are rapid in onset and take several weeks to be reversed. Some dog testes appear to be more sensitive to the damage and it may be irreversible in these testes.", "PMID": 955136}
{"id": "pubmed23n0032_1500", "title": "Vasectomy and vasovasostomy. II. A comparison of two methods of vasovasostomy: silastic versus chromic stents.", "content": "Twelve adult, bilaterally vasectomized dogs were used to compare two different procedures for reuniting the severed vas deferens. Approximately 5 months postvasectomy six dogs had vasovasostomies utilizing Silastic stents and the vasa deferentia of six additional dogs were rejoined with intravasal chromic stents. Ejaculates were subsequently obtained at 2-week intervals and evaluated for sperm count, motility, viability, and seminal ion concentrations. All of the dogs in which Silastic had been utilized as the stent had sperm reappear in the ejaculate, whereas only 40% of the dogs in which chromic catgut had been utilized had reappearance of spermatozoa. The over-all quality of the ejaculate was also better in the animals in which Silastic had been utilized. Testicular and epididymal histology was normal in most dogs of both groups, but three of the dogs with chromic stents in which the anastomosis had not been a success had abnormal histology, with reduced numbers of spermatocytes, spermatids, and spermatozoa. The data suggest that Silastic stents are better than chromic stents for vas reanastomosis. They also suggest that some dog testes react negatively to vasectomy and do not recover by 1 year postvasectomy when vas patency is not reversed.", "contents": "Vasectomy and vasovasostomy. II. A comparison of two methods of vasovasostomy: silastic versus chromic stents. Twelve adult, bilaterally vasectomized dogs were used to compare two different procedures for reuniting the severed vas deferens. Approximately 5 months postvasectomy six dogs had vasovasostomies utilizing Silastic stents and the vasa deferentia of six additional dogs were rejoined with intravasal chromic stents. Ejaculates were subsequently obtained at 2-week intervals and evaluated for sperm count, motility, viability, and seminal ion concentrations. All of the dogs in which Silastic had been utilized as the stent had sperm reappear in the ejaculate, whereas only 40% of the dogs in which chromic catgut had been utilized had reappearance of spermatozoa. The over-all quality of the ejaculate was also better in the animals in which Silastic had been utilized. Testicular and epididymal histology was normal in most dogs of both groups, but three of the dogs with chromic stents in which the anastomosis had not been a success had abnormal histology, with reduced numbers of spermatocytes, spermatids, and spermatozoa. The data suggest that Silastic stents are better than chromic stents for vas reanastomosis. They also suggest that some dog testes react negatively to vasectomy and do not recover by 1 year postvasectomy when vas patency is not reversed.", "PMID": 955137}
{"id": "pubmed23n0032_1501", "title": "The molecular weight of aspermatogenic antigen as determined by equilibrium ultracentrifugation.", "content": "The extraction of antigen from testis and sperm is described. This antigen, when administered in microgram amounts in a single injection, induces aspermatogenesis in 100% of guinea pigs so treated. The deletion of the seminiferous cell line is specific (no cross-reactions) and provides a tool for investigating inhibition of cell replication as well as reproductive control. The antigen is a polypeptide-polysaccharide complex and has been determined to have a molecular weight of 10,520, using the equilibrium ultracentrifugation method.", "contents": "The molecular weight of aspermatogenic antigen as determined by equilibrium ultracentrifugation. The extraction of antigen from testis and sperm is described. This antigen, when administered in microgram amounts in a single injection, induces aspermatogenesis in 100% of guinea pigs so treated. The deletion of the seminiferous cell line is specific (no cross-reactions) and provides a tool for investigating inhibition of cell replication as well as reproductive control. The antigen is a polypeptide-polysaccharide complex and has been determined to have a molecular weight of 10,520, using the equilibrium ultracentrifugation method.", "PMID": 955138}
{"id": "pubmed23n0032_1502", "title": "[Changes in the pulmonary ventilation of cats with a combination of increased respiratory resistance and hypercapnia before and after vagotomy].", "content": "In cat+s, increased resistance against breathing at the background of hyperventilation with a hypercapnic mixture reduces the 1-min ventilation. However, the bioelectric activity of the diaphragmatic nerve becomes augmented, thus indicating an increase in the respiratory center inspiratory activity. Vagotomy was followed by a decreased ventilatory response to CO2. Suppression of ventilation and augmentation of the diaphragmatic nerve activity during additional resistance against breathing in vagotomized animals were preserved on account of a considerably greater activity of the respiratory center. The data obtained suggest that, in conditions of combination of increased resistance against breathing with hypercapnia, the lungs afferent system fulfills an optimization of activity of the ventilatory apparatus.", "contents": "[Changes in the pulmonary ventilation of cats with a combination of increased respiratory resistance and hypercapnia before and after vagotomy]. In cat+s, increased resistance against breathing at the background of hyperventilation with a hypercapnic mixture reduces the 1-min ventilation. However, the bioelectric activity of the diaphragmatic nerve becomes augmented, thus indicating an increase in the respiratory center inspiratory activity. Vagotomy was followed by a decreased ventilatory response to CO2. Suppression of ventilation and augmentation of the diaphragmatic nerve activity during additional resistance against breathing in vagotomized animals were preserved on account of a considerably greater activity of the respiratory center. The data obtained suggest that, in conditions of combination of increased resistance against breathing with hypercapnia, the lungs afferent system fulfills an optimization of activity of the ventilatory apparatus.", "PMID": 955140}
{"id": "pubmed23n0032_1503", "title": "An enzymologic study of corpora lutea in early pregnant rats treated with abortifacient agents.", "content": "Luteal metabolism was investigated in corpora lutea of early pregnant rats treated with four abortifacient agents. In corpora lutea of rats treated with prostaglandin F2alpha or of rats 1 day postpartum, glucose-6-phosphate dehydrogenase (G6PDH) activity increased 140 to 170% and 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) was activated to significantly high levels, whereas malic enzyme activity decreased to 29% of control values. In aminoglutethimide-treated rats, the activities of G6PDH and malic enzyme decreased, while 20alpha-HSD activity was maintained at a very low level. With the increased dose, complete abortion was observed. In corpora lutea of these aborted rats, 20alpha-HSD was activated moderately and G6PDH values were slightly higher than control values, whereas malic enzyme activity fell to lower levels. All rats treated with clomiphene citrate aborted within 63 hours after the last injection. The activities of G6PDH, malic enzyme, and ATP citrate lyase in these corpora lutea decreased to 66, 68, and 72% of control levels, respectively; 20alpha-HSD activity was maintained at a very low level, and activities of 3beta-hydroxysteroid dehydrogenase, 6-phosphogluconate dehydrogenase, and pyruvate kinase were not appreciably altered. These findings indicated that, at the beginning of luteolysis and fetal resorption, the activities of steroidogenic enzymes decreased and 20alpha-HSD was not yet activated. Therefore, we could gauge the early changes of luteolysis by measuring the activities of G6PDH, MALIC ENZYME, AND ATP citrate lyase as well as 20alpha-HSD.", "contents": "An enzymologic study of corpora lutea in early pregnant rats treated with abortifacient agents. Luteal metabolism was investigated in corpora lutea of early pregnant rats treated with four abortifacient agents. In corpora lutea of rats treated with prostaglandin F2alpha or of rats 1 day postpartum, glucose-6-phosphate dehydrogenase (G6PDH) activity increased 140 to 170% and 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) was activated to significantly high levels, whereas malic enzyme activity decreased to 29% of control values. In aminoglutethimide-treated rats, the activities of G6PDH and malic enzyme decreased, while 20alpha-HSD activity was maintained at a very low level. With the increased dose, complete abortion was observed. In corpora lutea of these aborted rats, 20alpha-HSD was activated moderately and G6PDH values were slightly higher than control values, whereas malic enzyme activity fell to lower levels. All rats treated with clomiphene citrate aborted within 63 hours after the last injection. The activities of G6PDH, malic enzyme, and ATP citrate lyase in these corpora lutea decreased to 66, 68, and 72% of control levels, respectively; 20alpha-HSD activity was maintained at a very low level, and activities of 3beta-hydroxysteroid dehydrogenase, 6-phosphogluconate dehydrogenase, and pyruvate kinase were not appreciably altered. These findings indicated that, at the beginning of luteolysis and fetal resorption, the activities of steroidogenic enzymes decreased and 20alpha-HSD was not yet activated. Therefore, we could gauge the early changes of luteolysis by measuring the activities of G6PDH, MALIC ENZYME, AND ATP citrate lyase as well as 20alpha-HSD.", "PMID": 955139}
{"id": "pubmed23n0032_1504", "title": "[Reaction of the human ventilatory apparatus to supplementary respiratory resistance upon inhalation of normo- and hypercapnic mixtures].", "content": "In 5 healthy young subjects, the intrathoracic pressure, pneumotachogram, and CO2 tension in exhaled air were recorded--under conditions of breathing with additional resistance (15 cm water/1/sec). While breathing with air, starting from the 1st respiratory cycle after switching on of the additional resistance, a respiratory regimen had been formed (decreased ventilation and increased respiratory activity) which did not alter during next 10 min. When breathing with hypercapnic mixture (4% CO2 in air), the respiratory regimen changed in two stages: a stepwise increase of respiratory activity and reducing of ventilation, and further gradual increase of these parameters. Increase in the respiratory activity was invariably followed by an increase in the CO2 alveolar tension. Role of chemo- and mechanoreceptors in formation of the ventilatory apparatus response to additional resistance against breathing, is discussed.", "contents": "[Reaction of the human ventilatory apparatus to supplementary respiratory resistance upon inhalation of normo- and hypercapnic mixtures]. In 5 healthy young subjects, the intrathoracic pressure, pneumotachogram, and CO2 tension in exhaled air were recorded--under conditions of breathing with additional resistance (15 cm water/1/sec). While breathing with air, starting from the 1st respiratory cycle after switching on of the additional resistance, a respiratory regimen had been formed (decreased ventilation and increased respiratory activity) which did not alter during next 10 min. When breathing with hypercapnic mixture (4% CO2 in air), the respiratory regimen changed in two stages: a stepwise increase of respiratory activity and reducing of ventilation, and further gradual increase of these parameters. Increase in the respiratory activity was invariably followed by an increase in the CO2 alveolar tension. Role of chemo- and mechanoreceptors in formation of the ventilatory apparatus response to additional resistance against breathing, is discussed.", "PMID": 955141}
{"id": "pubmed23n0032_1505", "title": "[Response of the human ventilatory apparatus to additional respiratory resistance upon inhalation of normo- and hypercapnic mixtures].", "content": "In 78 healthy subjects, at 760 m (Frunze) and 3600 m (the Pamirs) above sea level, apneic points and ventilatory responses to increasing hypercapnia were assessed (rebreathing method). At 3600 m (4 to 11-month adaptation), regulation of breathing was achieved at lesser threshold CO2 values than in lowlanders, with no quantitative difference in ventilatory responses. On days 5 to 7 of deadaptation (at 760 m) following 45 days of adaptation at 3870 m, there was a decrease in ventilatory responses to a 1 mm Hg increment in PACO2, while apneic point values approached base-line values (prior to ascent to the high altitude).", "contents": "[Response of the human ventilatory apparatus to additional respiratory resistance upon inhalation of normo- and hypercapnic mixtures]. In 78 healthy subjects, at 760 m (Frunze) and 3600 m (the Pamirs) above sea level, apneic points and ventilatory responses to increasing hypercapnia were assessed (rebreathing method). At 3600 m (4 to 11-month adaptation), regulation of breathing was achieved at lesser threshold CO2 values than in lowlanders, with no quantitative difference in ventilatory responses. On days 5 to 7 of deadaptation (at 760 m) following 45 days of adaptation at 3870 m, there was a decrease in ventilatory responses to a 1 mm Hg increment in PACO2, while apneic point values approached base-line values (prior to ascent to the high altitude).", "PMID": 955142}
{"id": "pubmed23n0032_1506", "title": "[Ventilatory responses in man to hypoxia and hypercapnia under conditions of decreased respiratory resistance].", "content": "In young healthy male adults, a decrease in the mechanical load of the ventilatory apparatus was followed by readjustment in the respiratory cycle. During hyperventilation due to an enhanced hypercapnic stimulus, a reduction of resistance against breathing tells on the value of ventilatory response, the latter being greater than when breathing with mixtures of ordinary density. The data obtained confirm the significance of value of the mechanical load of respiratory apparatus for formation of ventilatory response to hypoxia and hypercapnia.", "contents": "[Ventilatory responses in man to hypoxia and hypercapnia under conditions of decreased respiratory resistance]. In young healthy male adults, a decrease in the mechanical load of the ventilatory apparatus was followed by readjustment in the respiratory cycle. During hyperventilation due to an enhanced hypercapnic stimulus, a reduction of resistance against breathing tells on the value of ventilatory response, the latter being greater than when breathing with mixtures of ordinary density. The data obtained confirm the significance of value of the mechanical load of respiratory apparatus for formation of ventilatory response to hypoxia and hypercapnia.", "PMID": 955143}
{"id": "pubmed23n0032_1507", "title": "[Reorganization of the function of several intrinsic laryngeal muscles as a result of exclusion of one of them during respiration].", "content": "Electromyographic data on the effect of unilateral extirpation of the cricoarytenoid posterior muscle on other intrinsic muscles activity and on the vocal fold mobility were obtained in a series of chronic experiments in dogs,--before the extirpation, immediately after it, and in 1.5, 3, 6, and more months. The respiration was recorded simultaneously with the EMG of the intrinsic laryngeal muscles and with laryngoscopy and photography of the larynx. The data obtained showed a decrease in the electric activity of the lateral and vocal muscles on the side of the extirpated cricoarytenoid posterior muscle within 1.5-3 months after the operation, and traces of this activity sometimes not only in expiration as well. In about 6 months or more after the extirpation, the electric activity of the lateral and vocal muscles on the side of the extirpation occurred not in expiration, as before the operation, but in inspiration only. The vocal fold on this side of the larynx was in the paramedian position and immobile at the state of respiration during the whole period after the extirpation. The reconstruction of activity of the larynx sphincter muscles after extirpation of dilatatory muscles may be a result of the central reconstruction of coordination of some motoneurons connected with different muscles of the larynx and may have a compensatory character. This reconstruction occurring after the cricoarytenoid posterior muscle extirpation provides most favourable conditions for the respiratory function of the larynx.", "contents": "[Reorganization of the function of several intrinsic laryngeal muscles as a result of exclusion of one of them during respiration]. Electromyographic data on the effect of unilateral extirpation of the cricoarytenoid posterior muscle on other intrinsic muscles activity and on the vocal fold mobility were obtained in a series of chronic experiments in dogs,--before the extirpation, immediately after it, and in 1.5, 3, 6, and more months. The respiration was recorded simultaneously with the EMG of the intrinsic laryngeal muscles and with laryngoscopy and photography of the larynx. The data obtained showed a decrease in the electric activity of the lateral and vocal muscles on the side of the extirpated cricoarytenoid posterior muscle within 1.5-3 months after the operation, and traces of this activity sometimes not only in expiration as well. In about 6 months or more after the extirpation, the electric activity of the lateral and vocal muscles on the side of the extirpation occurred not in expiration, as before the operation, but in inspiration only. The vocal fold on this side of the larynx was in the paramedian position and immobile at the state of respiration during the whole period after the extirpation. The reconstruction of activity of the larynx sphincter muscles after extirpation of dilatatory muscles may be a result of the central reconstruction of coordination of some motoneurons connected with different muscles of the larynx and may have a compensatory character. This reconstruction occurring after the cricoarytenoid posterior muscle extirpation provides most favourable conditions for the respiratory function of the larynx.", "PMID": 955144}
{"id": "pubmed23n0032_1508", "title": "[Dynamics of the process of gas transport into the body using a mathematical model].", "content": "Dynamics of mass-transport of oxygen, carbon dioxide, and inert gases in lungs, blood, and tissues, as well as gas transport through alveolar capillary and erythrocyte membranes at rest and during exercise under normal and increased ambient pressures, were studied on a mathematical model. The model consists of 34 differential and 58 algebraic equations and makes it possible to estimate the dynamics of changes of over 90 parameters. The effect of various factors: duration of the respiratory cycle, tidal volume, airways resistance, the surface of diffusion, the resistance of alveolar-capillary wall, erythrocyte membrane, ventilation-perfusion relations, pulmonary blood shunts, blood supply to the tissues, Haldane and Verigo-Bohr effect, buffer capacity of the blood, and others) on the mass-transport of gases were quantitatively estimated.", "contents": "[Dynamics of the process of gas transport into the body using a mathematical model]. Dynamics of mass-transport of oxygen, carbon dioxide, and inert gases in lungs, blood, and tissues, as well as gas transport through alveolar capillary and erythrocyte membranes at rest and during exercise under normal and increased ambient pressures, were studied on a mathematical model. The model consists of 34 differential and 58 algebraic equations and makes it possible to estimate the dynamics of changes of over 90 parameters. The effect of various factors: duration of the respiratory cycle, tidal volume, airways resistance, the surface of diffusion, the resistance of alveolar-capillary wall, erythrocyte membrane, ventilation-perfusion relations, pulmonary blood shunts, blood supply to the tissues, Haldane and Verigo-Bohr effect, buffer capacity of the blood, and others) on the mass-transport of gases were quantitatively estimated.", "PMID": 955147}
{"id": "pubmed23n0032_1509", "title": "[Changes in the oxygen tension of rat cerebral cortex subsequent to hyperoxia and the protective effect of urea].", "content": "When breathing with air, pO2 in anesthetized rats was 24.1+/-1.8 mm Hg, while after urea administration it was 19.4+/-1.7 mm Hg. A sharp increase in pO2 was revealed in hyperoxia(911+/-48 mm Hg at 6 atm during first 5 min, and 924+/-38 mm Hg--by the 7th min). The high pO2 was only reduced in 10-15 min after decompression. At a combined action of urea and hyperoxia, pO2 was significantly lower (714+/-52 mm Hg); pO2 in successive cortical layers (0-2400 mcm with a 200-mcm step) was progressively decreasing (from 40.7+/-4.9 mm Hg to 16.0+/-1.0 mm Hg in control, and from 928+/-35 mm Hg to 274+/-64 mm Hg in hyperoxia).", "contents": "[Changes in the oxygen tension of rat cerebral cortex subsequent to hyperoxia and the protective effect of urea]. When breathing with air, pO2 in anesthetized rats was 24.1+/-1.8 mm Hg, while after urea administration it was 19.4+/-1.7 mm Hg. A sharp increase in pO2 was revealed in hyperoxia(911+/-48 mm Hg at 6 atm during first 5 min, and 924+/-38 mm Hg--by the 7th min). The high pO2 was only reduced in 10-15 min after decompression. At a combined action of urea and hyperoxia, pO2 was significantly lower (714+/-52 mm Hg); pO2 in successive cortical layers (0-2400 mcm with a 200-mcm step) was progressively decreasing (from 40.7+/-4.9 mm Hg to 16.0+/-1.0 mm Hg in control, and from 928+/-35 mm Hg to 274+/-64 mm Hg in hyperoxia).", "PMID": 955149}
{"id": "pubmed23n0032_1510", "title": "[External respiration and oxygenation of arterial blood in animals in an atmosphere with an increased concentration of oxygen].", "content": "In 8 unanesthetized rabbits, the breathing rate, oxygenation of blood, and some other parameters were recorded during 1-hr stay in hyperoxic gas milieau with 40, 60, and 80% oxygen in nitrogen. In 40% mixture, the parameters changed insignificantly. In 60% oxygen, the 1-min respiratory volume first decreased then normalized while oxygenation of blood increased; in 80% mixture, both these parameters decreased due probably, to reduced ventilation, vasoconstrictive effect of oxygen, and shunting of blood in the lungs.", "contents": "[External respiration and oxygenation of arterial blood in animals in an atmosphere with an increased concentration of oxygen]. In 8 unanesthetized rabbits, the breathing rate, oxygenation of blood, and some other parameters were recorded during 1-hr stay in hyperoxic gas milieau with 40, 60, and 80% oxygen in nitrogen. In 40% mixture, the parameters changed insignificantly. In 60% oxygen, the 1-min respiratory volume first decreased then normalized while oxygenation of blood increased; in 80% mixture, both these parameters decreased due probably, to reduced ventilation, vasoconstrictive effect of oxygen, and shunting of blood in the lungs.", "PMID": 955150}
{"id": "pubmed23n0032_1511", "title": "[Functional capabilities of the papillary muscles of the left heart in rats during adaptation to high altitude conditions].", "content": "The amplitude of papillary muscles shortening at gradual increase of the rate of stimulation from 12 to 60, 120 180/min in conditions of permanent high altitude hypoxia on 3rd, 15th, 30th, and 45th day of adaptation noticeably increased at 180/min stimulation starting from the 15th day of mountain exposure and remaining till the 30th and 45th day. The performance of the muscles had been considerably decreased as compared with the control tests, on the 3rd day of adaptation.", "contents": "[Functional capabilities of the papillary muscles of the left heart in rats during adaptation to high altitude conditions]. The amplitude of papillary muscles shortening at gradual increase of the rate of stimulation from 12 to 60, 120 180/min in conditions of permanent high altitude hypoxia on 3rd, 15th, 30th, and 45th day of adaptation noticeably increased at 180/min stimulation starting from the 15th day of mountain exposure and remaining till the 30th and 45th day. The performance of the muscles had been considerably decreased as compared with the control tests, on the 3rd day of adaptation.", "PMID": 955153}
{"id": "pubmed23n0032_1512", "title": "[Regional redistribution of blood during air cooling of unanesthetized rats].", "content": "3-hr exposure to air at +5 degrees C of unrestrained rats evoked a relative reduction of blood volume in vessels of the skin, skeletal muscles, abdominal and pelvic organs (except liver). Increase of the blood volume occurred in the brain, thoracic organs, and in the liver. No significant decrease of the rectal temperature was noticed at that, in spite of cooling of the skin of different parts of the body and tail. Cooling of restrained rats at +5 degrees C during 1 and 3 hrs decreased the rectal temperature. The main direction of the blood redistribution involved its removal from the liver to the deep muscle tissues. At the beginning of the cooling and immediately after self--warming the relative increase of the blood volume was more obvious in the skeletal muscles of the anterior body parts.", "contents": "[Regional redistribution of blood during air cooling of unanesthetized rats]. 3-hr exposure to air at +5 degrees C of unrestrained rats evoked a relative reduction of blood volume in vessels of the skin, skeletal muscles, abdominal and pelvic organs (except liver). Increase of the blood volume occurred in the brain, thoracic organs, and in the liver. No significant decrease of the rectal temperature was noticed at that, in spite of cooling of the skin of different parts of the body and tail. Cooling of restrained rats at +5 degrees C during 1 and 3 hrs decreased the rectal temperature. The main direction of the blood redistribution involved its removal from the liver to the deep muscle tissues. At the beginning of the cooling and immediately after self--warming the relative increase of the blood volume was more obvious in the skeletal muscles of the anterior body parts.", "PMID": 955154}
{"id": "pubmed23n0032_1513", "title": "[Reactions of respiratory center neurons to local cooling of the ventral surface of the medulla oblongata].", "content": "In anesthetized cats, in conditions of vagal and sinus deafferentation, the effect of bilateral cooling of portions of the medulla oblongata ventral surface on electric activity of the respiratory volley neurons, was studied. The main types of the responses were as follows: cessation or reduction of firing, transformation of the volley discharges into continuous those or continuous with respiratory modulation. These responses were followed by cessation or sharp depression of respiration. Data on  localization of fields whose cooling entails maximal inhibitory responses of the respiratory neurons, are presented.", "contents": "[Reactions of respiratory center neurons to local cooling of the ventral surface of the medulla oblongata]. In anesthetized cats, in conditions of vagal and sinus deafferentation, the effect of bilateral cooling of portions of the medulla oblongata ventral surface on electric activity of the respiratory volley neurons, was studied. The main types of the responses were as follows: cessation or reduction of firing, transformation of the volley discharges into continuous those or continuous with respiratory modulation. These responses were followed by cessation or sharp depression of respiration. Data on  localization of fields whose cooling entails maximal inhibitory responses of the respiratory neurons, are presented.", "PMID": 955155}
{"id": "pubmed23n0032_1514", "title": "[Effect of transection of the forebrain, diencephalon and midbrain on respiration in cats].", "content": "In vagotomized cats, after removal of the brain portion 5 mm rostral to the visual nerves crossing, the eupnoea type of respiration was preserved. Transection in the area of the visual nerves crossing was followed by a stable acceleration of respiration. Starting from the level of the posterior border of the visual nerves crossing and more caudal, successive transections of the brain entailed sharp decrease in the breathing rate, increase of the respiratory volume, and complicated changes of the lungs ventilation.", "contents": "[Effect of transection of the forebrain, diencephalon and midbrain on respiration in cats]. In vagotomized cats, after removal of the brain portion 5 mm rostral to the visual nerves crossing, the eupnoea type of respiration was preserved. Transection in the area of the visual nerves crossing was followed by a stable acceleration of respiration. Starting from the level of the posterior border of the visual nerves crossing and more caudal, successive transections of the brain entailed sharp decrease in the breathing rate, increase of the respiratory volume, and complicated changes of the lungs ventilation.", "PMID": 955156}
{"id": "pubmed23n0032_1515", "title": "[Regulation of respiration under conditions of inhibition of carbonic anhydrase activity by diamox].", "content": "The functional state of the respiratory center and the mechanism of increase in pulmonary ventilation before and after inhibition of activity of carbonic anhydrase by diamox were studied in anesthetized cats. During the inhibition, activity of bulbar inspiratory neurons functionally connected with motoneurons of the diaphragm was markedly reduced and that of the neurons connected with motoneurons of intercostal muscles was sharply increased. A steady thoracic respiration occurred. The aferents from the carotid sinus nerve and vagus nerve did not affect the steady thoracic respiration. Increased efferent influence upon the intercostal muscles led to forced thoracic respiration which ensured a high pulmonary ventilation during the whole period of action of the diamox.", "contents": "[Regulation of respiration under conditions of inhibition of carbonic anhydrase activity by diamox]. The functional state of the respiratory center and the mechanism of increase in pulmonary ventilation before and after inhibition of activity of carbonic anhydrase by diamox were studied in anesthetized cats. During the inhibition, activity of bulbar inspiratory neurons functionally connected with motoneurons of the diaphragm was markedly reduced and that of the neurons connected with motoneurons of intercostal muscles was sharply increased. A steady thoracic respiration occurred. The aferents from the carotid sinus nerve and vagus nerve did not affect the steady thoracic respiration. Increased efferent influence upon the intercostal muscles led to forced thoracic respiration which ensured a high pulmonary ventilation during the whole period of action of the diamox.", "PMID": 955157}
{"id": "pubmed23n0032_1516", "title": "[Analysis of the imperative stimulus restricting voluntary breath holding].", "content": "In 5 healthy young men, the maximal voluntary breath holding--was studied under conditions of different initial values of PACO2--after oxygen inhalation at ordinary minute volume of respiration or at hyperventilation, or after breathing with oxygen mixtures with 2, 4, 6, 8 or 10% CO2. PACO2 was recorded at the moment of resuming of the intercostal muscles electric activity and at the point of breaking up the apnoea. A mathematical model was constructed. The hypothesis of participation of two independent factors (chemo- and mechanoreceptive) in genesis of the imperative stimulus for breathing was checked. The data obtained suggest that, in fact, the only factor restricting the duration of voluntary breath holding--is the summed up in time chemoreceptive stimulation. The involuntary contractions of respiratory muscles observed during apnoea are, apparently, a connecting link in formation of the imperative stimulus which breaks up the cessation of breathing.", "contents": "[Analysis of the imperative stimulus restricting voluntary breath holding]. In 5 healthy young men, the maximal voluntary breath holding--was studied under conditions of different initial values of PACO2--after oxygen inhalation at ordinary minute volume of respiration or at hyperventilation, or after breathing with oxygen mixtures with 2, 4, 6, 8 or 10% CO2. PACO2 was recorded at the moment of resuming of the intercostal muscles electric activity and at the point of breaking up the apnoea. A mathematical model was constructed. The hypothesis of participation of two independent factors (chemo- and mechanoreceptive) in genesis of the imperative stimulus for breathing was checked. The data obtained suggest that, in fact, the only factor restricting the duration of voluntary breath holding--is the summed up in time chemoreceptive stimulation. The involuntary contractions of respiratory muscles observed during apnoea are, apparently, a connecting link in formation of the imperative stimulus which breaks up the cessation of breathing.", "PMID": 955158}
{"id": "pubmed23n0032_1517", "title": "Length of residence, social ties, and economic opportunities,.", "content": "This study examines length of residence for movers--that subsection of the population which accounts for the high mobility rates in the United States. The propensity for repeated migration is studied in relation to economic opportunities and previous familial and personal contacts. The results indicate no influence of economic opportunities on duration of residence. This finding is consistent with recent research that indicates economic conditions are not a general stimulus for out-migration, although they are for inmigration. On the other hand, length of residence is found to be longer where previous familial and personal contacts existed. Overall, the results provide additional evidence of the importance of social, as opposed to strictly economic variables in the migration process.", "contents": "Length of residence, social ties, and economic opportunities,. This study examines length of residence for movers--that subsection of the population which accounts for the high mobility rates in the United States. The propensity for repeated migration is studied in relation to economic opportunities and previous familial and personal contacts. The results indicate no influence of economic opportunities on duration of residence. This finding is consistent with recent research that indicates economic conditions are not a general stimulus for out-migration, although they are for inmigration. On the other hand, length of residence is found to be longer where previous familial and personal contacts existed. Overall, the results provide additional evidence of the importance of social, as opposed to strictly economic variables in the migration process.", "PMID": 955169}
{"id": "pubmed23n0032_1518", "title": "The relation of psychological, social, and economic variables to fertility-related decisions.", "content": "To test a recent model of social behavior, a questionnaire was designed and administered to 270 married women in a midwestern city. A stratified random sample was obtained, comprising a 2 X 3 factorial design (Catholic vs. Protestant and high vs. middle vs. low socioeconomic status). It was hypothesized that an individual's intention to engage in a fertility-related behavior would be highly correlated with (a) her beliefs about the consequences of performing that behavior weighted by the value of those consequences, and/or (b) her beliefs about what relevant others think she should do and her motivation to comply with those others. The data strongly supported this hypothesis for each of three different behaviors. Analysis of variance revealed a significant religious differential for intentions to have a two-child family (Catholics were less likely to intend to have a two-child family than Protestants). This differential was further examined in terms of the proposed model of behavior.", "contents": "The relation of psychological, social, and economic variables to fertility-related decisions. To test a recent model of social behavior, a questionnaire was designed and administered to 270 married women in a midwestern city. A stratified random sample was obtained, comprising a 2 X 3 factorial design (Catholic vs. Protestant and high vs. middle vs. low socioeconomic status). It was hypothesized that an individual's intention to engage in a fertility-related behavior would be highly correlated with (a) her beliefs about the consequences of performing that behavior weighted by the value of those consequences, and/or (b) her beliefs about what relevant others think she should do and her motivation to comply with those others. The data strongly supported this hypothesis for each of three different behaviors. Analysis of variance revealed a significant religious differential for intentions to have a two-child family (Catholics were less likely to intend to have a two-child family than Protestants). This differential was further examined in terms of the proposed model of behavior.", "PMID": 955170}
{"id": "pubmed23n0032_1519", "title": "Female work experience, employment status, and birth expectations: sequential decision-making in the Philippines.", "content": "The influence of women's birth parity and accumulated market skills on their current labor force participation and birth expectations is examined within a sequential choice framework. Analysis of household data from the 1973 Philippines National Demographic Survey suggests these patterns: (a) women who have accumulated larger families work less in the current period and anticipate fewer additional births; (b) women with more past work experience tend to work more hours in the current period; and (c) work experience appears to have only a weak negative effect on birth expectations among older women.", "contents": "Female work experience, employment status, and birth expectations: sequential decision-making in the Philippines. The influence of women's birth parity and accumulated market skills on their current labor force participation and birth expectations is examined within a sequential choice framework. Analysis of household data from the 1973 Philippines National Demographic Survey suggests these patterns: (a) women who have accumulated larger families work less in the current period and anticipate fewer additional births; (b) women with more past work experience tend to work more hours in the current period; and (c) work experience appears to have only a weak negative effect on birth expectations among older women.", "PMID": 955171}
{"id": "pubmed23n0032_1520", "title": "Compensating changes in fertility and mortality.", "content": "This paper illustrates a method of studying changes in vital rate schedules which have no effect on the intrinsic rate of population growth. These changes are described as compensating changes in fertility and mortality. The analysis proceeds from the discrete perspective of Leslie matrices, wherein the central idea is to establish the set of all compensating changes by identifying that class of Leslie matrices which possess the same positive eigenvalue, lambda1. A root-squaring technique is adapted for the purpose of estimating lambda1. Finally, a variety of compensating fertility and mortality changes is illustrated using data from Japan.", "contents": "Compensating changes in fertility and mortality. This paper illustrates a method of studying changes in vital rate schedules which have no effect on the intrinsic rate of population growth. These changes are described as compensating changes in fertility and mortality. The analysis proceeds from the discrete perspective of Leslie matrices, wherein the central idea is to establish the set of all compensating changes by identifying that class of Leslie matrices which possess the same positive eigenvalue, lambda1. A root-squaring technique is adapted for the purpose of estimating lambda1. Finally, a variety of compensating fertility and mortality changes is illustrated using data from Japan.", "PMID": 955172}
{"id": "pubmed23n0032_1521", "title": "Report on a multiple regression method for making population estimates.", "content": "A new method of making population estimates is introduced and contrasted to the standard ratio-correlation method. The new method, called the \"difference-correlation method,\" produced population estimates for the counties of Michigan which had a smaller mean percentage error than estimates produced by the ratio-correlation method. The differences of proportions which are used in the difference-correlation method will always have means of zero, while the ratios used in the ratio-correlation method have means which vary. Higher intercorrelations among the variables and increased temporal stability of the intercorrelations are two advantages of using differences rather than ratios.", "contents": "Report on a multiple regression method for making population estimates. A new method of making population estimates is introduced and contrasted to the standard ratio-correlation method. The new method, called the \"difference-correlation method,\" produced population estimates for the counties of Michigan which had a smaller mean percentage error than estimates produced by the ratio-correlation method. The differences of proportions which are used in the difference-correlation method will always have means of zero, while the ratios used in the ratio-correlation method have means which vary. Higher intercorrelations among the variables and increased temporal stability of the intercorrelations are two advantages of using differences rather than ratios.", "PMID": 955173}
{"id": "pubmed23n0032_1522", "title": "Report on matching procedures of a dual record system in the southern Philippines.", "content": "There are generally three stages to the development of rules for matching vital events data from two sources covering the same population: (a) establishing a set of \"true\" matches and nonmatches; (b) determining the best tolerance limits for each single characteristic which might be used in matching; and (c) experimenting to determine the set or sets of characteristics and the weights to be used in classifying a pair of records as matched or nonmatched. Specific examples, based on early matching experiments with data from the dual record system of the Mindanao Center for Population Studies (MCPS), are presented. Successive application of different sets of characteristics (differential valence rule) to the remaining unmatched events produced an acceptable rule for matching in this study.", "contents": "Report on matching procedures of a dual record system in the southern Philippines. There are generally three stages to the development of rules for matching vital events data from two sources covering the same population: (a) establishing a set of \"true\" matches and nonmatches; (b) determining the best tolerance limits for each single characteristic which might be used in matching; and (c) experimenting to determine the set or sets of characteristics and the weights to be used in classifying a pair of records as matched or nonmatched. Specific examples, based on early matching experiments with data from the dual record system of the Mindanao Center for Population Studies (MCPS), are presented. Successive application of different sets of characteristics (differential valence rule) to the remaining unmatched events produced an acceptable rule for matching in this study.", "PMID": 955174}
{"id": "pubmed23n0032_1523", "title": "Provisional evaluation of the 1970 census count of American Indians.", "content": "Estimates of the American Indian population under 20 years of age on April 1, 1970, based on birth and death statistics for a 20-year period, show a possible net undercount of 6.9 percent for this age group in the 1970 census. However, for some particular ages the estimates indicate net overcounts in the census. Likewise, the net increase of the entire American Indian population as measured by the difference between the 1960 and 1970 censuses is 67,000 greater than the natural increase for the decade. Detailed analysis of cohort data with respect to the possible causes of the differences between the estimates and the census figures indicate that a portion of the estimated net overcounts can be attributed to classification, as well as coverage, problems. The estimated net overcounts offer support for the hypothesis that many individuals who were registered as white at birth and who were counted as white in the 1960 census shifted their racial self-identification from white to American Indian during the 1960's.", "contents": "Provisional evaluation of the 1970 census count of American Indians. Estimates of the American Indian population under 20 years of age on April 1, 1970, based on birth and death statistics for a 20-year period, show a possible net undercount of 6.9 percent for this age group in the 1970 census. However, for some particular ages the estimates indicate net overcounts in the census. Likewise, the net increase of the entire American Indian population as measured by the difference between the 1960 and 1970 censuses is 67,000 greater than the natural increase for the decade. Detailed analysis of cohort data with respect to the possible causes of the differences between the estimates and the census figures indicate that a portion of the estimated net overcounts can be attributed to classification, as well as coverage, problems. The estimated net overcounts offer support for the hypothesis that many individuals who were registered as white at birth and who were counted as white in the 1960 census shifted their racial self-identification from white to American Indian during the 1960's.", "PMID": 955175}
{"id": "pubmed23n0032_1524", "title": "Sweat studies in hyperhidrosis palmaris and plantaris. A survey of 60 patients before and after cervical sympathectomy.", "content": "60 patients suffering from excessive sweating in the hands underwent cervical sympathectomy. At follow-up 1-7 years after operation the hands were dry in 55. No differences in electrolyte concentrations were found in the sweat from palms, arms, or body prior to and after the surgical intervention. Similarly no differences were found in electrolyte concentrations between healthy subjects and hyperhidrotics. Palmar sweat was hypertonic in comparison to arm or body sweat. The Na and K sweat concentrations were: palmar 50 +/- 20 and 16 +/- 7 mEq/1; arm 30 +/- 11 and 8 +/- 2 mEq/1; and body 21 +/- 20 and 4 +/- 3 mEq/1, respectively. Na/K ratio was: palmar 3.2; arms 3.6 and body 5.8. Total sweat loss prior to the operation was 5.7 +/- 4.2 g/kg BW/h; afterwards it was 5.9 +/- 4 g/kg BW/h. These equal sweat rates were associated with compensatory sweating in areas of the body not affected prior to the operation.", "contents": "Sweat studies in hyperhidrosis palmaris and plantaris. A survey of 60 patients before and after cervical sympathectomy. 60 patients suffering from excessive sweating in the hands underwent cervical sympathectomy. At follow-up 1-7 years after operation the hands were dry in 55. No differences in electrolyte concentrations were found in the sweat from palms, arms, or body prior to and after the surgical intervention. Similarly no differences were found in electrolyte concentrations between healthy subjects and hyperhidrotics. Palmar sweat was hypertonic in comparison to arm or body sweat. The Na and K sweat concentrations were: palmar 50 +/- 20 and 16 +/- 7 mEq/1; arm 30 +/- 11 and 8 +/- 2 mEq/1; and body 21 +/- 20 and 4 +/- 3 mEq/1, respectively. Na/K ratio was: palmar 3.2; arms 3.6 and body 5.8. Total sweat loss prior to the operation was 5.7 +/- 4.2 g/kg BW/h; afterwards it was 5.9 +/- 4 g/kg BW/h. These equal sweat rates were associated with compensatory sweating in areas of the body not affected prior to the operation.", "PMID": 955224}
{"id": "pubmed23n0032_1525", "title": "[Pattern of epithelium formation in human and guinea-pig skin (author's transl)].", "content": "Comparative histometry of human and guinea-pig epidermis revealed that in the latter development of the (subcorneal) superficial epithelium is significantly less, and that of the (mostly follicular) deep epithelium is markedly more pronounced than in man.", "contents": "[Pattern of epithelium formation in human and guinea-pig skin (author's transl)]. Comparative histometry of human and guinea-pig epidermis revealed that in the latter development of the (subcorneal) superficial epithelium is significantly less, and that of the (mostly follicular) deep epithelium is markedly more pronounced than in man.", "PMID": 955225}
{"id": "pubmed23n0032_1526", "title": "[Basophils in the infiltrate of guinea pig experimental contact dermatitis. Dinitrochlorobenzene and citraconic anhydride (author's transl)].", "content": "We observed, in addition to the lymphocytes of the classical descriptions, large numbers of basophils in the infiltrate of the specific sensitization reaction with dinitrochlorobenzene and citraconic acid, a few eosinophils in the reaction with dinitrochlorobenzene and rather more eosinophils in the reaction with citraconic acid.", "contents": "[Basophils in the infiltrate of guinea pig experimental contact dermatitis. Dinitrochlorobenzene and citraconic anhydride (author's transl)]. We observed, in addition to the lymphocytes of the classical descriptions, large numbers of basophils in the infiltrate of the specific sensitization reaction with dinitrochlorobenzene and citraconic acid, a few eosinophils in the reaction with dinitrochlorobenzene and rather more eosinophils in the reaction with citraconic acid.", "PMID": 955226}
{"id": "pubmed23n0032_1527", "title": "The mechanism of photochemotherapy.", "content": "As a contribution to the mechanisms of photochemotherapy, human skin homogenates were irradiated in the presence or absence of methoxsalen. The changes induced in LDH-, G-6-PDH-, GAPDH-, and GOT-activities were registered. Methoxsalen (50 mug/ml) failed to produce any significant effect. On pure G-6-PDH, methoxsalen exhibited a photoprotective action.", "contents": "The mechanism of photochemotherapy. As a contribution to the mechanisms of photochemotherapy, human skin homogenates were irradiated in the presence or absence of methoxsalen. The changes induced in LDH-, G-6-PDH-, GAPDH-, and GOT-activities were registered. Methoxsalen (50 mug/ml) failed to produce any significant effect. On pure G-6-PDH, methoxsalen exhibited a photoprotective action.", "PMID": 955227}
{"id": "pubmed23n0032_1528", "title": "[In vitro and in vivo experiments on the immunosuppressive properties of cytostatic substances (author's transl)].", "content": "Various cystostatic substances are tested with the lymphocyte transformation test and compared with the effect on the experimental contact eczema of the guinea pig. Only methotrexate influences the eczematous reaction. The in vitro and in vivo effects are not identical.", "contents": "[In vitro and in vivo experiments on the immunosuppressive properties of cytostatic substances (author's transl)]. Various cystostatic substances are tested with the lymphocyte transformation test and compared with the effect on the experimental contact eczema of the guinea pig. Only methotrexate influences the eczematous reaction. The in vitro and in vivo effects are not identical.", "PMID": 955228}
{"id": "pubmed23n0032_1529", "title": "[Photosensitization of the skin and fluorescence spectra (author's transl)].", "content": "We have carried out spectrofluorometric determinations of numerous substances such as drugs, chemicals and plants in order to find out whether there is a correlation between fluorescence data and photosensitization of the skin. A hypothesis on the mechanism of photosensitization is discussed and the data on the fluorescent properties of some 50 products is given.", "contents": "[Photosensitization of the skin and fluorescence spectra (author's transl)]. We have carried out spectrofluorometric determinations of numerous substances such as drugs, chemicals and plants in order to find out whether there is a correlation between fluorescence data and photosensitization of the skin. A hypothesis on the mechanism of photosensitization is discussed and the data on the fluorescent properties of some 50 products is given.", "PMID": 955229}
{"id": "pubmed23n0032_1530", "title": "Therapy of resistant psoriasis with topical corticosteroids and dimethylsulfoxide.", "content": "The sequential application of full-strength dimethylsulfoxide (DMSO) and potent topical corticosteroid preparations was very effective in resistant plaque-type psoriasis. Complete clearing may be achieved in 3-4 weeks. Lower strengths of DMSO were less beneficial. Irritation from the solvent was effectively controlled by corticosteroids and was limited to transient burning or stinging. Resistance to topical corticosteroids may be overcome by the concomitant use of DMSO.", "contents": "Therapy of resistant psoriasis with topical corticosteroids and dimethylsulfoxide. The sequential application of full-strength dimethylsulfoxide (DMSO) and potent topical corticosteroid preparations was very effective in resistant plaque-type psoriasis. Complete clearing may be achieved in 3-4 weeks. Lower strengths of DMSO were less beneficial. Irritation from the solvent was effectively controlled by corticosteroids and was limited to transient burning or stinging. Resistance to topical corticosteroids may be overcome by the concomitant use of DMSO.", "PMID": 955230}
{"id": "pubmed23n0032_1531", "title": "Particle size of dexamethasone incorporated in ointment and its behaviour during penetration in the stratum corneum of living skin.", "content": "The particle size of dexamethasone was microscopically determined in polarized light in two emulsion ointments: a standard water-in-old formula (aqueous wool alcohol ointment), and a standard oil-in-water formula (aqueous hydrophilic ointment), both containing 0.023% dexamethasone. The distribution of dexamethasone was on average smaller and finer in the water-in-oil emulsion than in the oil-in-water emulsion. In a further series of experiments we studied by means of the adhesive tape-stripping method the proportions of the particle size and counted the total number of particles in various layers of the stratum corneum in vivo. The difference between the two emulsion types once again proved to be significant, but only as far as the epidermal barrier. A new, uniform particle size spectrum was formed at this level, having a maximum value of 24 mum, which, irrespective of the type of ointment, allows a release of the desamethasone in the live epidermis and dermis. The original particle size distribution is thus qualitatively insignificant in so far as the therapeutic effect of dexamethasone below the barrier zone is concerned. Since the particle size has a strongly distorted frequency distribution, with amarked shift to the left, only about 0.5% of the particles in the w/o emulsion and only about 2% in the o/w emulsion are in fact larger than 56mum, the maximum value measured lying just below the surface of the horny layer. Any loss during further penetration is probably negligible.", "contents": "Particle size of dexamethasone incorporated in ointment and its behaviour during penetration in the stratum corneum of living skin. The particle size of dexamethasone was microscopically determined in polarized light in two emulsion ointments: a standard water-in-old formula (aqueous wool alcohol ointment), and a standard oil-in-water formula (aqueous hydrophilic ointment), both containing 0.023% dexamethasone. The distribution of dexamethasone was on average smaller and finer in the water-in-oil emulsion than in the oil-in-water emulsion. In a further series of experiments we studied by means of the adhesive tape-stripping method the proportions of the particle size and counted the total number of particles in various layers of the stratum corneum in vivo. The difference between the two emulsion types once again proved to be significant, but only as far as the epidermal barrier. A new, uniform particle size spectrum was formed at this level, having a maximum value of 24 mum, which, irrespective of the type of ointment, allows a release of the desamethasone in the live epidermis and dermis. The original particle size distribution is thus qualitatively insignificant in so far as the therapeutic effect of dexamethasone below the barrier zone is concerned. Since the particle size has a strongly distorted frequency distribution, with amarked shift to the left, only about 0.5% of the particles in the w/o emulsion and only about 2% in the o/w emulsion are in fact larger than 56mum, the maximum value measured lying just below the surface of the horny layer. Any loss during further penetration is probably negligible.", "PMID": 955231}
{"id": "pubmed23n0032_1532", "title": "In vivo percutaneous penetration of corticoids in man and unresolved problems in their efficacy.", "content": "This paper review the percutaneous penetration of corticosteroids in vivo in man utilizing urinary excretion of 14C-hydrocortisone as the experimental method. In general corticosteroids are only minimally absorbed from most areas of glabrous skin. Increasing the amount that penetrates-by use of occlusion-and increasing concentration provides the most practical method currently available for increasing efficacy in treatment-resistant dermatoses. Some practical research problems relevant to topical corticoid efficacy are discussed.", "contents": "In vivo percutaneous penetration of corticoids in man and unresolved problems in their efficacy. This paper review the percutaneous penetration of corticosteroids in vivo in man utilizing urinary excretion of 14C-hydrocortisone as the experimental method. In general corticosteroids are only minimally absorbed from most areas of glabrous skin. Increasing the amount that penetrates-by use of occlusion-and increasing concentration provides the most practical method currently available for increasing efficacy in treatment-resistant dermatoses. Some practical research problems relevant to topical corticoid efficacy are discussed.", "PMID": 955232}
{"id": "pubmed23n0032_1533", "title": "Objective measurement of topically applied corticosteroids.", "content": "The vasoconstriction caused by four topical corticosteroid preparations was compared according to subjective ranking and according to measurement by a tristimulus colourimeter (Momcolor). The measured results agreed well with visual evaluation. The colourimeter was proved to be measuring the degree of vasoconstriction. Among the preparations examined, the vasoconstriction effected by hydrocortisone 17-butyrate cream (Locoid) and by betamethasone dipropionate ointment (Diprosone) was stronger than that exerted by triamcinolone acetonide (Ftorocort) and fluocinolone acetonide (Flucinar). With the aid of colourimetric measurement of the vasoconstriction, differences could be demonstrated between clinically effective topical corticosteroid preparations. An open question remains, what the predictive significance is of the objectively measured vasoconstriction test, for clinical practice.", "contents": "Objective measurement of topically applied corticosteroids. The vasoconstriction caused by four topical corticosteroid preparations was compared according to subjective ranking and according to measurement by a tristimulus colourimeter (Momcolor). The measured results agreed well with visual evaluation. The colourimeter was proved to be measuring the degree of vasoconstriction. Among the preparations examined, the vasoconstriction effected by hydrocortisone 17-butyrate cream (Locoid) and by betamethasone dipropionate ointment (Diprosone) was stronger than that exerted by triamcinolone acetonide (Ftorocort) and fluocinolone acetonide (Flucinar). With the aid of colourimetric measurement of the vasoconstriction, differences could be demonstrated between clinically effective topical corticosteroid preparations. An open question remains, what the predictive significance is of the objectively measured vasoconstriction test, for clinical practice.", "PMID": 955233}
{"id": "pubmed23n0032_1534", "title": "Topical and systemic effects of hydrocortisone 17-butyrate.", "content": "In a series of hydrocortisone 17-esters, hydrocortisone 17-butyrate (HC-17B) has almost optimal lipophilicity resulting in a topical effect comparable to that of the fluorinated corticosteroids as measured in the McKenzie test on the human skin. In rats its systemic effects are weak, being in the order of those of hydrocortisone. In man systemic effects have been demonstrated, but no exact comparative. In man systemic effects have been demonstrated, but no exact comparative data are available. Important FACTORS controlling the intensity of systemic effects are: rate of liberation into plasma from the depot in the horny LAYER, RATE of metabolic degradation, and rate of plasma clearance. In rats the main factor seems to be rapid clearance from plasma (t 1/2 = 0.5h) by selective concentration in the liver and, to a lesser extent, in the kidneys. Rapid hydrolysis by esterases in plasma and liver plays an additional role. In man hydrolysis by esterases appears to be rather slow; the rate of clearance from plasma is still unknown, whereas the absorption from the intact skin into the blood seems to be very slow (t 1/2 = 25 h).", "contents": "Topical and systemic effects of hydrocortisone 17-butyrate. In a series of hydrocortisone 17-esters, hydrocortisone 17-butyrate (HC-17B) has almost optimal lipophilicity resulting in a topical effect comparable to that of the fluorinated corticosteroids as measured in the McKenzie test on the human skin. In rats its systemic effects are weak, being in the order of those of hydrocortisone. In man systemic effects have been demonstrated, but no exact comparative. In man systemic effects have been demonstrated, but no exact comparative data are available. Important FACTORS controlling the intensity of systemic effects are: rate of liberation into plasma from the depot in the horny LAYER, RATE of metabolic degradation, and rate of plasma clearance. In rats the main factor seems to be rapid clearance from plasma (t 1/2 = 0.5h) by selective concentration in the liver and, to a lesser extent, in the kidneys. Rapid hydrolysis by esterases in plasma and liver plays an additional role. In man hydrolysis by esterases appears to be rather slow; the rate of clearance from plasma is still unknown, whereas the absorption from the intact skin into the blood seems to be very slow (t 1/2 = 25 h).", "PMID": 955234}
{"id": "pubmed23n0032_1535", "title": "Systemic effects and percutaneous absorption of topically applied 0.1% hydrocortisone 17-butyrate.", "content": "The systemic effects of topically applied 0.1% hydrocortisone 17-butyrate during treatment with and without occlusion were measured in terms of the functions of the pituitary-adrenal axis. Corticosteroid levels in the blood were determined by the modified method of MURPHY, a competitive protein-binding radioassay. Circulating eosinophils were counted and the urinary 17-OHCS and 17-KS excretion were also determined. Percutaneous absorption following the topical application of 14C hydrocortisone 17-butyrate 1 (HC 17-B, Locoid) was measured by means of autoradiography. The application of the corticosteroid to the normal skin without occlusion did not induce systemic effects, whilst the application of the drug under occlusive dressing showed that systemic effects may possibly be induced. The number of circulating eosinophils, corticosteroid levels in the blood and urinary 17-OHCS excretion all decreased. However, these values returned to normal about 2 days after the withdrawal of the application, so we consider the systemic effect induced by the topical corticosteroid to be of a temporary nature. The percutaneous absorption of the corticosteroid was proved by an autoradiographic technique using 14C-labelled HC 17-B. At various intervals after the application, radioactive substances were observed as silver grains in the horny layer of the epidermis and skin appendages, and the density of the silver grains gradually increased with the course of time. It may be surmised that locally applied HC 17-B will be retained in the horny layer of the epidermis at a very early stage after the application and then absorbed in the blood via the epidermis and skin appendages. The reservoir of the corticosteroid in the skin was demonstrated 24 h after the withdrawal of the application.", "contents": "Systemic effects and percutaneous absorption of topically applied 0.1% hydrocortisone 17-butyrate. The systemic effects of topically applied 0.1% hydrocortisone 17-butyrate during treatment with and without occlusion were measured in terms of the functions of the pituitary-adrenal axis. Corticosteroid levels in the blood were determined by the modified method of MURPHY, a competitive protein-binding radioassay. Circulating eosinophils were counted and the urinary 17-OHCS and 17-KS excretion were also determined. Percutaneous absorption following the topical application of 14C hydrocortisone 17-butyrate 1 (HC 17-B, Locoid) was measured by means of autoradiography. The application of the corticosteroid to the normal skin without occlusion did not induce systemic effects, whilst the application of the drug under occlusive dressing showed that systemic effects may possibly be induced. The number of circulating eosinophils, corticosteroid levels in the blood and urinary 17-OHCS excretion all decreased. However, these values returned to normal about 2 days after the withdrawal of the application, so we consider the systemic effect induced by the topical corticosteroid to be of a temporary nature. The percutaneous absorption of the corticosteroid was proved by an autoradiographic technique using 14C-labelled HC 17-B. At various intervals after the application, radioactive substances were observed as silver grains in the horny layer of the epidermis and skin appendages, and the density of the silver grains gradually increased with the course of time. It may be surmised that locally applied HC 17-B will be retained in the horny layer of the epidermis at a very early stage after the application and then absorbed in the blood via the epidermis and skin appendages. The reservoir of the corticosteroid in the skin was demonstrated 24 h after the withdrawal of the application.", "PMID": 955235}
{"id": "pubmed23n0032_1536", "title": "Effect of hydrocortisone 17-butyrate on the percutaneous absorption of antibiotics.", "content": "Abrption of antibiotics from combined preparations containing oxytetracycline, chlortetracycline, doxycycline or gentamicin and hydrocortisone 17-butyrate into the skin of rats was examined. The penetration of antibiotics from the ointment into the skin was inhibited by 0.2% hydrocortisone 17-butyrate to a significant degree. It is suggested that this effect of hydrocortisone 17-butyrate is the consequence of its vasoconstrictor action.", "contents": "Effect of hydrocortisone 17-butyrate on the percutaneous absorption of antibiotics. Abrption of antibiotics from combined preparations containing oxytetracycline, chlortetracycline, doxycycline or gentamicin and hydrocortisone 17-butyrate into the skin of rats was examined. The penetration of antibiotics from the ointment into the skin was inhibited by 0.2% hydrocortisone 17-butyrate to a significant degree. It is suggested that this effect of hydrocortisone 17-butyrate is the consequence of its vasoconstrictor action.", "PMID": 955236}
{"id": "pubmed23n0032_1537", "title": "Penetration of corticosteroids through the skin in relation to the vehicle.", "content": "Results of in vitro studies on the penetration of a corticosteroid hydrocortisone 17-butyrate (HCN) in relation to the vehicle are reported. When HCB is dissolved completely in the vehicle, penetration is enhanced, and only under this condition an increase in HCB concentration (in the range 0.05-0.2%) leads to an increase in the penetration. Evaluation of the efficiency of various vehicles (an oil/water cream, Plastibase or gels) in which HCB was dissolved in propylene glycol or ethanol, showed that Plastibase with propylene glycol was the most effective for both 0.1 and 0.2% HCB. The results of experiments in which HCB was applied in ethanolic solutions suggests that at least initially HCB and ethanol together penetrate through the epidermis.", "contents": "Penetration of corticosteroids through the skin in relation to the vehicle. Results of in vitro studies on the penetration of a corticosteroid hydrocortisone 17-butyrate (HCN) in relation to the vehicle are reported. When HCB is dissolved completely in the vehicle, penetration is enhanced, and only under this condition an increase in HCB concentration (in the range 0.05-0.2%) leads to an increase in the penetration. Evaluation of the efficiency of various vehicles (an oil/water cream, Plastibase or gels) in which HCB was dissolved in propylene glycol or ethanol, showed that Plastibase with propylene glycol was the most effective for both 0.1 and 0.2% HCB. The results of experiments in which HCB was applied in ethanolic solutions suggests that at least initially HCB and ethanol together penetrate through the epidermis.", "PMID": 955237}
{"id": "pubmed23n0032_1538", "title": "Bioavailability of topical steroids.", "content": "This paper review our modification of the vasoconstrictor (blanching) assay for topical corticosteroids using human volunteers, which we have improved and extended so that it may be used as a useful screening test for clinical efficacy besides being employed to determine the bioavailability of steroids from topical bases. Work is reported on the blanching activities of (1) 14 novel steroids in ethanol, (2)hydrocortisone 17-butyrate in eight topical vehicles, (3) betamethasone 17-benzoate in foam, ointment, cream and gel bases, and (4) 61 commercial formulations used in Great Britain. Pharmacokinetic/pharmacodynamic relationships are discussed as aids to optimizing topical steroid treatment.", "contents": "Bioavailability of topical steroids. This paper review our modification of the vasoconstrictor (blanching) assay for topical corticosteroids using human volunteers, which we have improved and extended so that it may be used as a useful screening test for clinical efficacy besides being employed to determine the bioavailability of steroids from topical bases. Work is reported on the blanching activities of (1) 14 novel steroids in ethanol, (2)hydrocortisone 17-butyrate in eight topical vehicles, (3) betamethasone 17-benzoate in foam, ointment, cream and gel bases, and (4) 61 commercial formulations used in Great Britain. Pharmacokinetic/pharmacodynamic relationships are discussed as aids to optimizing topical steroid treatment.", "PMID": 955238}
{"id": "pubmed23n0032_1539", "title": "Effects of local corticosteroids in skin infections.", "content": "Glucocorticoids exert valuable therapeutic activities in skin infections, although their use is limited by the occurrence of undesiderable effects: glucocorticoids depress the local defense mechanisms and in low concentrations, stimulate microbiol metabolism. For these reasons, combined preparations containing an antimicrobiol substance besides the glucocorticoid are recommended. However, interactions between the steroids and the antimicrobiols must be ruled out beforehand, i.e. the unimpaired activity of both steroid and antimicrobial must be assured. Examples of such investigations are reported for the most widely used compounds for topical therapy. The simultaneous use of a glucocorticoid and an antimicrobial is not recommended in only a few instances.", "contents": "Effects of local corticosteroids in skin infections. Glucocorticoids exert valuable therapeutic activities in skin infections, although their use is limited by the occurrence of undesiderable effects: glucocorticoids depress the local defense mechanisms and in low concentrations, stimulate microbiol metabolism. For these reasons, combined preparations containing an antimicrobiol substance besides the glucocorticoid are recommended. However, interactions between the steroids and the antimicrobiols must be ruled out beforehand, i.e. the unimpaired activity of both steroid and antimicrobial must be assured. Examples of such investigations are reported for the most widely used compounds for topical therapy. The simultaneous use of a glucocorticoid and an antimicrobial is not recommended in only a few instances.", "PMID": 955239}
{"id": "pubmed23n0032_1540", "title": "Effect of bases and accelerants on the anti-inflammatory activity of topical corticosteroids.", "content": "The use of measurement of skin thickness in guinea pigs has been compared with other techniques for assessment of topical applications. This method has provided us with an accurate evaluation of the corticosteroid dose-response curve and the effect of bases. The method has shown that non-steroid prostaglandin synthesis inhibitors can also be demonstrated to possess topical anti-inflammatory activity. As expected, indomethacin acts synergistically when formulated with hydrocortisone. Contrary to our expectation, both salicylic acid and acetylsalicylic acid show antagonism with hydrocortisone. This finding may be of clinical significance. We have also found that a combination of salicylic acid and indomethacin produces striking inflammatory changes in guinea pig skin which points to interaction of these two drugs in the guinea pig. The relevance of this phenomenon to both the mode of action of the two drugs and their use in clinical practice remains to be elucidated, but preliminary tests suggest that similar changes take place in human skin.", "contents": "Effect of bases and accelerants on the anti-inflammatory activity of topical corticosteroids. The use of measurement of skin thickness in guinea pigs has been compared with other techniques for assessment of topical applications. This method has provided us with an accurate evaluation of the corticosteroid dose-response curve and the effect of bases. The method has shown that non-steroid prostaglandin synthesis inhibitors can also be demonstrated to possess topical anti-inflammatory activity. As expected, indomethacin acts synergistically when formulated with hydrocortisone. Contrary to our expectation, both salicylic acid and acetylsalicylic acid show antagonism with hydrocortisone. This finding may be of clinical significance. We have also found that a combination of salicylic acid and indomethacin produces striking inflammatory changes in guinea pig skin which points to interaction of these two drugs in the guinea pig. The relevance of this phenomenon to both the mode of action of the two drugs and their use in clinical practice remains to be elucidated, but preliminary tests suggest that similar changes take place in human skin.", "PMID": 955240}
{"id": "pubmed23n0032_1541", "title": "Vasoconstriction in response to corticosteroids observed in human lips.", "content": "Following epimucous application of corticosteroids on the lips, narrowing and shortening of the terminal network of vessels occurs within 5-7 min. The decrease in infrared radiation needs a few more minutes. Contrary to the application of epinephrine-like substances, the change of the vessels occurs first in the superficial layers and only later on in the deeper layers. In addition to the effect on the muscles of the vessels, a narrowing of the capillaries can also be influenced by the effect of corticosteroids on the endothelial cells. Changes of the ground substance of the capillary vessel bed are also a possibility.", "contents": "Vasoconstriction in response to corticosteroids observed in human lips. Following epimucous application of corticosteroids on the lips, narrowing and shortening of the terminal network of vessels occurs within 5-7 min. The decrease in infrared radiation needs a few more minutes. Contrary to the application of epinephrine-like substances, the change of the vessels occurs first in the superficial layers and only later on in the deeper layers. In addition to the effect on the muscles of the vessels, a narrowing of the capillaries can also be influenced by the effect of corticosteroids on the endothelial cells. Changes of the ground substance of the capillary vessel bed are also a possibility.", "PMID": 955241}
{"id": "pubmed23n0032_1542", "title": "Thyroid thermogenesis: minimal contribution of energy requirement for protein synthesis.", "content": "Cycloheximide (5.0 mug/ml) had no significant effect on respiration of liver slices prepared from euthyroid rats (i.e., QO2 of 15.5 +/- 0.8 vs. 14.9 +/- 0.8 mul/mg protein) despite an 83.8 +/- 2.1% inhibition of protein synthesis as judged by [3H]leucine incorporation into the trichloroacetic acid precipitable fraction. Injection of triiodothyronine increased the QO2 of rat liver slices to 22.6 +/- 1.1 mul/h/mg protein. The QO2 of hyperthyroid slices remained high in the presence of cycloheximide although protein synthesis was inhibited by 84.4 +/- 2.0%. These results imply that the energy requirement for protein synthesis contributes little to QO2 of euthyroid liver and does not account for a significant fraction of the increase in hepatic QO2 obtained in the transition from the euthyroid to the hyperthyroid state.", "contents": "Thyroid thermogenesis: minimal contribution of energy requirement for protein synthesis. Cycloheximide (5.0 mug/ml) had no significant effect on respiration of liver slices prepared from euthyroid rats (i.e., QO2 of 15.5 +/- 0.8 vs. 14.9 +/- 0.8 mul/mg protein) despite an 83.8 +/- 2.1% inhibition of protein synthesis as judged by [3H]leucine incorporation into the trichloroacetic acid precipitable fraction. Injection of triiodothyronine increased the QO2 of rat liver slices to 22.6 +/- 1.1 mul/h/mg protein. The QO2 of hyperthyroid slices remained high in the presence of cycloheximide although protein synthesis was inhibited by 84.4 +/- 2.0%. These results imply that the energy requirement for protein synthesis contributes little to QO2 of euthyroid liver and does not account for a significant fraction of the increase in hepatic QO2 obtained in the transition from the euthyroid to the hyperthyroid state.", "PMID": 955245}
{"id": "pubmed23n0032_1543", "title": "Dynamics of steroid uptake in rat testis studied by quantitative autoradiography.", "content": "Localization of radioactive steroids in rat testis was studied by autoradiography of tissue section. For autoradiography small tissue samples were frozen, freeze-dried under vacuum, fixed with osmium vapor and embedded in epon. The transfer of radioactive steroids was studied after in vitro perfusion of radioactive steroids in the testes isolated from hypophysectomized animals, thereby excluding the interference of endogenous steroids. Quantitative autoradiography on the basis of grain densities after perfusion of testes with tritiated pregnenolone or testosterone, revealed an accumulation of the label in the Leydig cell cytoplasm. After longer perfusion periods the amount of label in the seminiferous tubules increased and a preferential localization was observed in the basal cytoplasm of Sertoli cells and in lipid droplets. Perfusion of testes with estradiol-17 beta resulted in a distinctly different pattern of radioactivity in the autoradiographs. A high labeling of the Leydig cell nuclei was observed in combination with a low general labeling of all the other cell structures. The results suggest that different steroids are localized in different specific areas of the rat testes in vivo.", "contents": "Dynamics of steroid uptake in rat testis studied by quantitative autoradiography. Localization of radioactive steroids in rat testis was studied by autoradiography of tissue section. For autoradiography small tissue samples were frozen, freeze-dried under vacuum, fixed with osmium vapor and embedded in epon. The transfer of radioactive steroids was studied after in vitro perfusion of radioactive steroids in the testes isolated from hypophysectomized animals, thereby excluding the interference of endogenous steroids. Quantitative autoradiography on the basis of grain densities after perfusion of testes with tritiated pregnenolone or testosterone, revealed an accumulation of the label in the Leydig cell cytoplasm. After longer perfusion periods the amount of label in the seminiferous tubules increased and a preferential localization was observed in the basal cytoplasm of Sertoli cells and in lipid droplets. Perfusion of testes with estradiol-17 beta resulted in a distinctly different pattern of radioactivity in the autoradiographs. A high labeling of the Leydig cell nuclei was observed in combination with a low general labeling of all the other cell structures. The results suggest that different steroids are localized in different specific areas of the rat testes in vivo.", "PMID": 955244}
{"id": "pubmed23n0032_1544", "title": "Transport of thyroglobulin and peroxidase in the thyroid follicle cell.", "content": "The purpose of this study was to explore the nature of the protein(s) in the exocytotic vesicles in the thyroid follicle cells and to ascertain whether or not thyroglobulin and peroxidase are transported by the same vesicles through the apical region of the cells to the follicle lumen. The study was performed on rats pretreated with thyroxine for 2 days in order to inhibit endocytosis. A fraction of exocytotic vesicles was isolated by centrifugation in continuous and discontinuous sucrose density gradients. The protein content of the vesicles were analysed by electrophoresis in continuous polyacrylamide gradient gels. The vesicles contained (uniodinated) thyroglobulin, 12-S protein and thyralbumin. Parallel histochemical studies in the electron microscope. These observations have important bearings on the mechanisms for thyroglobulin iodination, since it has been demonstrated that iodination does not occur in the exocytotic vesicles but in connection with the opening of the vesicles at the apical cell surface.", "contents": "Transport of thyroglobulin and peroxidase in the thyroid follicle cell. The purpose of this study was to explore the nature of the protein(s) in the exocytotic vesicles in the thyroid follicle cells and to ascertain whether or not thyroglobulin and peroxidase are transported by the same vesicles through the apical region of the cells to the follicle lumen. The study was performed on rats pretreated with thyroxine for 2 days in order to inhibit endocytosis. A fraction of exocytotic vesicles was isolated by centrifugation in continuous and discontinuous sucrose density gradients. The protein content of the vesicles were analysed by electrophoresis in continuous polyacrylamide gradient gels. The vesicles contained (uniodinated) thyroglobulin, 12-S protein and thyralbumin. Parallel histochemical studies in the electron microscope. These observations have important bearings on the mechanisms for thyroglobulin iodination, since it has been demonstrated that iodination does not occur in the exocytotic vesicles but in connection with the opening of the vesicles at the apical cell surface.", "PMID": 955246}
{"id": "pubmed23n0032_1545", "title": "Hormonal control of vitellogenin synthesis in avian liver.", "content": "Estradiol induces the synthesis of vitellogenin in the avian liver. We describe the precursor-product relationship between vitellogenin and the yolk proteins phosvitin and lipovitellin. The high rate of vitellogenin synthesis is a consequence of the accumulation of a stable messenger RNA. We suggest that estradiol acts at the level of the genome by opening a hitherto non-transcribed gene.", "contents": "Hormonal control of vitellogenin synthesis in avian liver. Estradiol induces the synthesis of vitellogenin in the avian liver. We describe the precursor-product relationship between vitellogenin and the yolk proteins phosvitin and lipovitellin. The high rate of vitellogenin synthesis is a consequence of the accumulation of a stable messenger RNA. We suggest that estradiol acts at the level of the genome by opening a hitherto non-transcribed gene.", "PMID": 955247}
{"id": "pubmed23n0032_1546", "title": "Effect of prolactin on vitamin D metabolism.", "content": "The effect of ovine prolactin on the renal 25-hydroxycholecalciferol-1-hydroxylase was studied in the chick. Prolactin was found to increase the activity of this enzyme in both long-term and short-term experiments. In the long term, 7 days treatment with prolactin caused a marked stimulation of the 1-hydroxylase activity, however this effect was only seen when the enzyme was assayed 2-3 hours after the final injection of prolactin. A single subcutaneous injection of prolactin was also effective in increasing the 1-hydroxylase activity, this effect was maximal at one hour and had largely disappeared 3 hours after prolactin administration.", "contents": "Effect of prolactin on vitamin D metabolism. The effect of ovine prolactin on the renal 25-hydroxycholecalciferol-1-hydroxylase was studied in the chick. Prolactin was found to increase the activity of this enzyme in both long-term and short-term experiments. In the long term, 7 days treatment with prolactin caused a marked stimulation of the 1-hydroxylase activity, however this effect was only seen when the enzyme was assayed 2-3 hours after the final injection of prolactin. A single subcutaneous injection of prolactin was also effective in increasing the 1-hydroxylase activity, this effect was maximal at one hour and had largely disappeared 3 hours after prolactin administration.", "PMID": 955248}
{"id": "pubmed23n0032_1547", "title": "RNA synthesis in different stages of rat seminiferous epithelial cycle.", "content": "In vitro RNA synthesis has been analyzed using autoradiographic and electrophoretic methods in isolated 1-2 mm segments of rat seminiferous tubules. The cellular composition of each segment was accurately identified using microscopic analysis of the transillumination pattern of the freshly isolated, unstained, seminiferous tubules combined with phase-contrast microscopy of the living spermatogenic cells. The RNA synthesized in the seminiferous tubules was found to be mostly heterogenous nuclear RNA (HnRNA), which appeared to have a long lifetime. It was most actively formed in the stages which contain mid-pachytene spermatocytes. Formation of rRNA was slow in all stages and it was first observed when a 2-h pulse with [3H]uridine was followed by a 6-h chase. A very low RNA synthetic rate was observed in the stage containing the meiotic reduction divisions. The function of the meiotic RNA in regulation of spermiogenesis is discussed.", "contents": "RNA synthesis in different stages of rat seminiferous epithelial cycle. In vitro RNA synthesis has been analyzed using autoradiographic and electrophoretic methods in isolated 1-2 mm segments of rat seminiferous tubules. The cellular composition of each segment was accurately identified using microscopic analysis of the transillumination pattern of the freshly isolated, unstained, seminiferous tubules combined with phase-contrast microscopy of the living spermatogenic cells. The RNA synthesized in the seminiferous tubules was found to be mostly heterogenous nuclear RNA (HnRNA), which appeared to have a long lifetime. It was most actively formed in the stages which contain mid-pachytene spermatocytes. Formation of rRNA was slow in all stages and it was first observed when a 2-h pulse with [3H]uridine was followed by a 6-h chase. A very low RNA synthetic rate was observed in the stage containing the meiotic reduction divisions. The function of the meiotic RNA in regulation of spermiogenesis is discussed.", "PMID": 955250}
{"id": "pubmed23n0032_1548", "title": "Uptake of biogenic amines by thyroid glands.", "content": "The biogenic amines, serotonin (5-HT) and norepinephrine, activate follicular cells and are endogenous thyroid constitutents. Both amines are taken up by throid lobes incubated in vitro. Uptake of 5-HT is temperature and concentration dependent, is inhibited by chlorimipramine more than by desmethylimipramine, and although it is inhibited by norepinephrine, 5-HT uptake is unaffected by chemical sympathetomy with 6-hydroxydopamine. Electron microscopic radioautography reveals that follicular cells are responsible for uptake of 5-HT and adrenergic nerves for uptake of norepinephrine. These data are consistent with the hypothesis that biogenic amines are intrathyroidal transmitters acting on follicular cells, and that uptake mechanisms may contribute to their inactivation.", "contents": "Uptake of biogenic amines by thyroid glands. The biogenic amines, serotonin (5-HT) and norepinephrine, activate follicular cells and are endogenous thyroid constitutents. Both amines are taken up by throid lobes incubated in vitro. Uptake of 5-HT is temperature and concentration dependent, is inhibited by chlorimipramine more than by desmethylimipramine, and although it is inhibited by norepinephrine, 5-HT uptake is unaffected by chemical sympathetomy with 6-hydroxydopamine. Electron microscopic radioautography reveals that follicular cells are responsible for uptake of 5-HT and adrenergic nerves for uptake of norepinephrine. These data are consistent with the hypothesis that biogenic amines are intrathyroidal transmitters acting on follicular cells, and that uptake mechanisms may contribute to their inactivation.", "PMID": 955249}
{"id": "pubmed23n0032_1549", "title": "Induction of avidin in the chick oviduct by tissue damage. Effect of promethazine chloride, CaCl2 and hydrocortisone on local induction.", "content": "The local effect of the mechanical induction of avidin by ligature was studied in diethylstilbestrol-primed chicks. The highest induction of avidin was always found in the immediate vicinity of the silk ligature of the oviduct. The locality of the induction was highly dependent on the position of the ligature. The nonligated parts of the ligated oviduct also showed a slight avidin induction. These results indicate a strictly local effect of avidin induction by ligature. An antihistamine, promethazine chloride, has a potentiating effect on the avidin induction by ligature when administered after the ligature. On the other hand, membrane stabilization by hydrocortisone or CaCl2 did not influence the ligature-induced avidin synthesis. On the basis of these results it is concluded that the avidin induction is not mediated by histamine activation or membrane damage.", "contents": "Induction of avidin in the chick oviduct by tissue damage. Effect of promethazine chloride, CaCl2 and hydrocortisone on local induction. The local effect of the mechanical induction of avidin by ligature was studied in diethylstilbestrol-primed chicks. The highest induction of avidin was always found in the immediate vicinity of the silk ligature of the oviduct. The locality of the induction was highly dependent on the position of the ligature. The nonligated parts of the ligated oviduct also showed a slight avidin induction. These results indicate a strictly local effect of avidin induction by ligature. An antihistamine, promethazine chloride, has a potentiating effect on the avidin induction by ligature when administered after the ligature. On the other hand, membrane stabilization by hydrocortisone or CaCl2 did not influence the ligature-induced avidin synthesis. On the basis of these results it is concluded that the avidin induction is not mediated by histamine activation or membrane damage.", "PMID": 955251}
{"id": "pubmed23n0032_1550", "title": "Chemical composition of porcine thyroid cell plasma membranes.", "content": "Plasma membranes were isolated from thyroid cells obtained by trypsinization of porcine glands and maintained in culture conditions in the presence or absence of thyrotropin or dibutyryl cyclic AMP. The protein, phospholipid, cholesterol and sialic acid content of the 3 types of cell plasma membranes were very similar. High cholesterol and sialic acid content characterized these membranes. The amino acid and carbohydrate composition was similar to that shown for other eukaryotic plasma membranes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis disclosed the presence of more than 20 protein bands, of which six corresponded to glycoproteins.", "contents": "Chemical composition of porcine thyroid cell plasma membranes. Plasma membranes were isolated from thyroid cells obtained by trypsinization of porcine glands and maintained in culture conditions in the presence or absence of thyrotropin or dibutyryl cyclic AMP. The protein, phospholipid, cholesterol and sialic acid content of the 3 types of cell plasma membranes were very similar. High cholesterol and sialic acid content characterized these membranes. The amino acid and carbohydrate composition was similar to that shown for other eukaryotic plasma membranes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis disclosed the presence of more than 20 protein bands, of which six corresponded to glycoproteins.", "PMID": 955252}
{"id": "pubmed23n0032_1551", "title": "Possibilities of immunization against cholera and related enterotoxic enteropathies.", "content": "Scientifically controlled field studies have established that parenterally administered killed vibrio vaccines or somatic antigen preparations offer only limited degrees of protection in certain population groups and have  made it obvious that new approaches to the immunoprophylaxis of cholera are needed. It has now also been established that the symptoms of cholera result from the action of the cholera enterotoxin (choleragen) on the epithelial cells of the small intestine.  Immulogically related enterotoxins have been incriminated in other newly recognized diarrheal diseases (e.g., those caused by Escherichia coli and \"non-agglutinable\" (NAG)vibrios). Additionally, volunteer studies have shown that induced cholera results in rather solid and lasting immunity against homologous re-challenge thus proving that immunity against cholera is feasible. Although numerous studies have demonstrated the efficacy of purely antitoxic immunity in experimental animal models, the protective effect of parenterally administered glutaraldehyde treated toxoid in man has shown to be limited, at best. The protection attained following an attack of cholera suggests that local immune  mechanism may be of predominant importance. Immunity has  been stimulated, experimentally in mice, by toxin antigen administered per os on a single occasion. Choleragenoid, which is non-toxic but binds to the same receptors as cholera toxin, has been shown to provide immediate resistance as well as later immunity to toxin challenge. More ideal, however, would be a colonizing mutant of V. cholerae which elaborates a non-toxic cross-reactive material (CRM) like choleragenoid and which could stimulate local antitoxic as well as anti-vibrio immune mechanisms. A tox-mutant of V. cholerae which is avirulent for man has been shown to elicit substantial immunity in man but the ideal CRM-mutant has yet to be found.", "contents": "Possibilities of immunization against cholera and related enterotoxic enteropathies. Scientifically controlled field studies have established that parenterally administered killed vibrio vaccines or somatic antigen preparations offer only limited degrees of protection in certain population groups and have  made it obvious that new approaches to the immunoprophylaxis of cholera are needed. It has now also been established that the symptoms of cholera result from the action of the cholera enterotoxin (choleragen) on the epithelial cells of the small intestine.  Immulogically related enterotoxins have been incriminated in other newly recognized diarrheal diseases (e.g., those caused by Escherichia coli and \"non-agglutinable\" (NAG)vibrios). Additionally, volunteer studies have shown that induced cholera results in rather solid and lasting immunity against homologous re-challenge thus proving that immunity against cholera is feasible. Although numerous studies have demonstrated the efficacy of purely antitoxic immunity in experimental animal models, the protective effect of parenterally administered glutaraldehyde treated toxoid in man has shown to be limited, at best. The protection attained following an attack of cholera suggests that local immune  mechanism may be of predominant importance. Immunity has  been stimulated, experimentally in mice, by toxin antigen administered per os on a single occasion. Choleragenoid, which is non-toxic but binds to the same receptors as cholera toxin, has been shown to provide immediate resistance as well as later immunity to toxin challenge. More ideal, however, would be a colonizing mutant of V. cholerae which elaborates a non-toxic cross-reactive material (CRM) like choleragenoid and which could stimulate local antitoxic as well as anti-vibrio immune mechanisms. A tox-mutant of V. cholerae which is avirulent for man has been shown to elicit substantial immunity in man but the ideal CRM-mutant has yet to be found.", "PMID": 955264}
{"id": "pubmed23n0032_1552", "title": "Influenza.", "content": "The author reminds us of the significance of hemagglutinin and neuraminidase, as well as their respective antibodies, in the development of influenza in man and other organisms. These factors are certainly not the only ones responsible for clinical symptomatology. The value of killed or attenuated virus vaccines is then discussed, together with the application of the viruses at the portal of entry, antigenic recombinants and particularly the elaboration of a \"master strain\" of virus (for example \"Alice\", WRL 105). Particular attention is devoted to the ts mutants, their genetic stability, transmissibility and protecting power against wild strains. The virus which Chanock and his associates have developed during the last few years offers considerable advantages; nevertheless continued research by several teams is suggested in order to improve still further the qualities of this virus.", "contents": "Influenza. The author reminds us of the significance of hemagglutinin and neuraminidase, as well as their respective antibodies, in the development of influenza in man and other organisms. These factors are certainly not the only ones responsible for clinical symptomatology. The value of killed or attenuated virus vaccines is then discussed, together with the application of the viruses at the portal of entry, antigenic recombinants and particularly the elaboration of a \"master strain\" of virus (for example \"Alice\", WRL 105). Particular attention is devoted to the ts mutants, their genetic stability, transmissibility and protecting power against wild strains. The virus which Chanock and his associates have developed during the last few years offers considerable advantages; nevertheless continued research by several teams is suggested in order to improve still further the qualities of this virus.", "PMID": 955265}
{"id": "pubmed23n0032_1553", "title": "Pathogenicity, immunogenicity and genetic stability in mice and ferrets of a cold inhibitor-resistant mutant of influenza H3N2 virus.", "content": "A cold inhibitor-resistant mutant was derived from MRC-2 virus by means of serial passages in the presence of rabbit serum and at gradually decreasing temperatures. This virus, denoted MRC-2 IRc, grew well at both 26 and 37 degree C. The pathogenicity and immunogenicity of the virus was examined in mice and ferrets. No clinical reactions were detected in either species. Both mice and ferrets developed high levels of hemagglutination inhibition and neuraminidase inhibition antibodies. The virus was reisolated from the lungs of intranasally inoculated mice; in ferrets it was recovered from nasal washing and trachea but not from lungs. The properties of the isolated viruses were examined. The viruses reisolated from mice lost their reproductive capacity at 26 degree C and were less resistant to inhibitors than the original virus. The virus isolated from the ferret nose was identical with the original virus, however the tracheal isolate exhibited a certain degree of reversion in both the markers tested.", "contents": "Pathogenicity, immunogenicity and genetic stability in mice and ferrets of a cold inhibitor-resistant mutant of influenza H3N2 virus. A cold inhibitor-resistant mutant was derived from MRC-2 virus by means of serial passages in the presence of rabbit serum and at gradually decreasing temperatures. This virus, denoted MRC-2 IRc, grew well at both 26 and 37 degree C. The pathogenicity and immunogenicity of the virus was examined in mice and ferrets. No clinical reactions were detected in either species. Both mice and ferrets developed high levels of hemagglutination inhibition and neuraminidase inhibition antibodies. The virus was reisolated from the lungs of intranasally inoculated mice; in ferrets it was recovered from nasal washing and trachea but not from lungs. The properties of the isolated viruses were examined. The viruses reisolated from mice lost their reproductive capacity at 26 degree C and were less resistant to inhibitors than the original virus. The virus isolated from the ferret nose was identical with the original virus, however the tracheal isolate exhibited a certain degree of reversion in both the markers tested.", "PMID": 955266}
{"id": "pubmed23n0032_1554", "title": "Preparation and characterization of live recombinant influenza vaccine.", "content": "Strain WRL 105 was prepared by recombination between the A/Finland/4/74 (H3N2) and attenuated A/Okuda/57 (H2N2) strains by a method previously described. A single intranasal dose of WRL 105 strain live attenuated influenza vaccine was administered to volunteers at three dose levels and homologous serum antibody titres and virus excretion were monitored. Doses greater than 10(6.3)EID50 gave 4-fold or greater increases in antibody titre in 80% of seronegative volunteers.", "contents": "Preparation and characterization of live recombinant influenza vaccine. Strain WRL 105 was prepared by recombination between the A/Finland/4/74 (H3N2) and attenuated A/Okuda/57 (H2N2) strains by a method previously described. A single intranasal dose of WRL 105 strain live attenuated influenza vaccine was administered to volunteers at three dose levels and homologous serum antibody titres and virus excretion were monitored. Doses greater than 10(6.3)EID50 gave 4-fold or greater increases in antibody titre in 80% of seronegative volunteers.", "PMID": 955267}
{"id": "pubmed23n0032_1555", "title": "Recombination of influenza A virus strains: effect on pathogenicity.", "content": "Influenza viruses can recombine genetic information, and progeny virus can be selected for desired genetic traits. A newly isolated strain can develop the ability to grow to higher titer in embryonated eggs by acquiring this trait from the AOPR8(HON1) strain, and can be selected by the terminal dilution technique and by treatment with anti-HON1 antisera. In addition to acquiring the ability to grow to high titer, surface antigens  may also be transferred, and the progeny can be selected by immunologic methods. It appeared likely that other genes, including those coding for virulence or attenuation might also be exchanged during the recombination procedure. We explored this  question by infecting mice with recent strains of H3N2 influenza virus, and with some of their progeny which had been selected for high virus yields from eggs, after recombination with the AOPR8(HON1) virus, a strain known to be virulent for mice. Influenza (H3N2) virus strains A/Scotland, A/Port Chalmers and A/England which  were isolated and passaged in embryonated eggs did not cause death when administered intranasally to three-week-old Swiss mice; however, after recombination with the A/PR8(HON1) virus, these viruses become lethal in mice. This acquisition of virulence appears to be secondary to exchange of genetic information from the parent AOPR8 virus. Virus isolated from  mice infected with these recombinants is antigenically H3N2, the mice develop anti-H3N2 antibodies.", "contents": "Recombination of influenza A virus strains: effect on pathogenicity. Influenza viruses can recombine genetic information, and progeny virus can be selected for desired genetic traits. A newly isolated strain can develop the ability to grow to higher titer in embryonated eggs by acquiring this trait from the AOPR8(HON1) strain, and can be selected by the terminal dilution technique and by treatment with anti-HON1 antisera. In addition to acquiring the ability to grow to high titer, surface antigens  may also be transferred, and the progeny can be selected by immunologic methods. It appeared likely that other genes, including those coding for virulence or attenuation might also be exchanged during the recombination procedure. We explored this  question by infecting mice with recent strains of H3N2 influenza virus, and with some of their progeny which had been selected for high virus yields from eggs, after recombination with the AOPR8(HON1) virus, a strain known to be virulent for mice. Influenza (H3N2) virus strains A/Scotland, A/Port Chalmers and A/England which  were isolated and passaged in embryonated eggs did not cause death when administered intranasally to three-week-old Swiss mice; however, after recombination with the A/PR8(HON1) virus, these viruses become lethal in mice. This acquisition of virulence appears to be secondary to exchange of genetic information from the parent AOPR8 virus. Virus isolated from  mice infected with these recombinants is antigenically H3N2, the mice develop anti-H3N2 antibodies.", "PMID": 955268}
{"id": "pubmed23n0032_1556", "title": "Response of ferrets  to infection with a live attenuated influenza virus and to subsequent heterologous challenge.", "content": "Groups of three ferrets were inoculated with a live influenza vaccine (\"Alice\" strain) and the degree of protection afforded against a challenge inoculation was measured by several parameters. After vaccination, virus excretion was studied and the genetic characteristics of the virus isolates were investigated. After 5-8 weeks, the challenge inoculations were  performed with antigenic drifts of the H3N2 virus: A/Dunedin/4/73  (Port-Chalmers-like), A/England/635/74 and A/Scotland/840/74. The following parameters of infection were evaluated: temperature rises, clinical symptoms, viral excretion, serological responses and increases in the nasal protein contents (Potter et. al(1972). Immunity to influenza in ferrets. I. Response to live and killed virus.  Brit. J. Exp. Path., 53, 153-167.) A very high degree of protection was observed in the immunized animals.", "contents": "Response of ferrets  to infection with a live attenuated influenza virus and to subsequent heterologous challenge. Groups of three ferrets were inoculated with a live influenza vaccine (\"Alice\" strain) and the degree of protection afforded against a challenge inoculation was measured by several parameters. After vaccination, virus excretion was studied and the genetic characteristics of the virus isolates were investigated. After 5-8 weeks, the challenge inoculations were  performed with antigenic drifts of the H3N2 virus: A/Dunedin/4/73  (Port-Chalmers-like), A/England/635/74 and A/Scotland/840/74. The following parameters of infection were evaluated: temperature rises, clinical symptoms, viral excretion, serological responses and increases in the nasal protein contents (Potter et. al(1972). Immunity to influenza in ferrets. I. Response to live and killed virus.  Brit. J. Exp. Path., 53, 153-167.) A very high degree of protection was observed in the immunized animals.", "PMID": 955269}
{"id": "pubmed23n0032_1557", "title": "Intranasal versus subcutaneous rubella vaccination in schoolgirls.", "content": "In this study a venous blood specimen was drawn and, at the same time, rubella vaccine was given to 1906 schoolgirls mostly in the pre-pubertal age. To assess the pre-vaccination immune status and the effect of the vaccine, a  second  blood sample was taken 6 weeks later and tested for rubella HAI antibody simultaneously with the first one. RA 27/3 rubella vaccine administered by intranasal route to 81 girls produced a 100% seroconversion rate and the same vaccine strain, given by subcutaneous route to 460 girls,  produced a 94.73% seroconversion rate. Among the 1, 365 schoolgirls who received subcutaneous Cendehill vaccine, the seroconversion rate was 86.68%. Side effects were mild and transient and occurred, as headache, most frequently among the girls who received RA 27/3 vaccine by intranasal route.", "contents": "Intranasal versus subcutaneous rubella vaccination in schoolgirls. In this study a venous blood specimen was drawn and, at the same time, rubella vaccine was given to 1906 schoolgirls mostly in the pre-pubertal age. To assess the pre-vaccination immune status and the effect of the vaccine, a  second  blood sample was taken 6 weeks later and tested for rubella HAI antibody simultaneously with the first one. RA 27/3 rubella vaccine administered by intranasal route to 81 girls produced a 100% seroconversion rate and the same vaccine strain, given by subcutaneous route to 460 girls,  produced a 94.73% seroconversion rate. Among the 1, 365 schoolgirls who received subcutaneous Cendehill vaccine, the seroconversion rate was 86.68%. Side effects were mild and transient and occurred, as headache, most frequently among the girls who received RA 27/3 vaccine by intranasal route.", "PMID": 955270}
{"id": "pubmed23n0032_1558", "title": "Dry aerosol vaccination against Newcastle disease. I. Safety and activity controls on chickens.", "content": "A new technic or immunization against Newcastle disease in chicken was developed: this method  consists of dispersing lyophilized products by aerosols. One to fourteen day old chickens, with and without parental antibodies, were vaccinated with increasing amounts (10(-1) to 10(5)EID50) of HB1 and LaSota attenuated viruses. 10(4)EID50, inhaled theoretical dose, gave better results when the animals were challenged with the virulent strain. Under laboratory conditions, no respiratory problems  occurred in vaccinated animals; but there is 10 to 20% mortality among animals treated by aerosols in poultry houses, because of respiratory diseases developed after vaccination.", "contents": "Dry aerosol vaccination against Newcastle disease. I. Safety and activity controls on chickens. A new technic or immunization against Newcastle disease in chicken was developed: this method  consists of dispersing lyophilized products by aerosols. One to fourteen day old chickens, with and without parental antibodies, were vaccinated with increasing amounts (10(-1) to 10(5)EID50) of HB1 and LaSota attenuated viruses. 10(4)EID50, inhaled theoretical dose, gave better results when the animals were challenged with the virulent strain. Under laboratory conditions, no respiratory problems  occurred in vaccinated animals; but there is 10 to 20% mortality among animals treated by aerosols in poultry houses, because of respiratory diseases developed after vaccination.", "PMID": 955271}
{"id": "pubmed23n0032_1559", "title": "Research and development of unconventional systems for immunization.", "content": "The problems raised by the ever expanding world population justify the work of USAID (U.S. Agency for International Development). Continual efforts must be made to develop and efficiently operate methods of control of the microbial and parasitic infections which menace our sources of protein. This programme includes immunization, technical methods in bacterial and viral genetics to obtain attenuated strains, and improvements in production, preservation and distribution of preparations. An example of  an unconventional but promising system of application is the pilot study recently begun in the Missouri-Mississippi River region.", "contents": "Research and development of unconventional systems for immunization. The problems raised by the ever expanding world population justify the work of USAID (U.S. Agency for International Development). Continual efforts must be made to develop and efficiently operate methods of control of the microbial and parasitic infections which menace our sources of protein. This programme includes immunization, technical methods in bacterial and viral genetics to obtain attenuated strains, and improvements in production, preservation and distribution of preparations. An example of  an unconventional but promising system of application is the pilot study recently begun in the Missouri-Mississippi River region.", "PMID": 955272}
{"id": "pubmed23n0032_1560", "title": "Dry aerosol vaccination against Newcastle disease: II. Serological response in chicks.", "content": "Day-old chickens with Newcastle disease maternal antibodies are separated in two batches. One batch (S) is vaccinated by liquid spray method with HB1 strain: the other one (NS) is not vaccinated. At 2, 3 or 4 weeks subgroups from S and NS batches are vaccinated with HB1 strain by dry aerosol method. Serological controls reveal an important increase of Newcastle antibodies within two weeks after dry aerosol vaccination. Then a drop of the antibody quantities is observed during weeks 2 to 4 post dry aerosol vaccination. In the following weeks, the antibody level increases very slowly.", "contents": "Dry aerosol vaccination against Newcastle disease: II. Serological response in chicks. Day-old chickens with Newcastle disease maternal antibodies are separated in two batches. One batch (S) is vaccinated by liquid spray method with HB1 strain: the other one (NS) is not vaccinated. At 2, 3 or 4 weeks subgroups from S and NS batches are vaccinated with HB1 strain by dry aerosol method. Serological controls reveal an important increase of Newcastle antibodies within two weeks after dry aerosol vaccination. Then a drop of the antibody quantities is observed during weeks 2 to 4 post dry aerosol vaccination. In the following weeks, the antibody level increases very slowly.", "PMID": 955273}
{"id": "pubmed23n0032_1561", "title": "Aerosol administration of Newcastle disease vaccines at one day of age.", "content": "Newcastle disease vaccines were administered at one day of age by 3 general procedures: 1) discharge of a vaccine aerosol over chicks confined in an enclosed space, 2) aerosol adminstration in a poultry house, 3) a commercial device which emits a fine spray into the nasopharynx while the chick is being debeaked. From this work the following conclusions have been drawn: 1) All 3 methods are of some value with respect to the development of antibody titers and resistance to challenge. 2) The efficacy is universely proportional to the maternal antibody titer. 3) Aerosol vaccination into an enclosed air space is superior to the other methods tested. 4) Day-old vaccination is useful primarily in conferring some early resistance until a subsequent aerosol vaccination at 9-14 days of age confers additional protection. Other important factors include strain of vaccine, vaccine titer, the nature of the diluent, and site of the aerolized particles.", "contents": "Aerosol administration of Newcastle disease vaccines at one day of age. Newcastle disease vaccines were administered at one day of age by 3 general procedures: 1) discharge of a vaccine aerosol over chicks confined in an enclosed space, 2) aerosol adminstration in a poultry house, 3) a commercial device which emits a fine spray into the nasopharynx while the chick is being debeaked. From this work the following conclusions have been drawn: 1) All 3 methods are of some value with respect to the development of antibody titers and resistance to challenge. 2) The efficacy is universely proportional to the maternal antibody titer. 3) Aerosol vaccination into an enclosed air space is superior to the other methods tested. 4) Day-old vaccination is useful primarily in conferring some early resistance until a subsequent aerosol vaccination at 9-14 days of age confers additional protection. Other important factors include strain of vaccine, vaccine titer, the nature of the diluent, and site of the aerolized particles.", "PMID": 955274}
{"id": "pubmed23n0032_1562", "title": "Studies on vaccination against Newcastle disease with the \"Komarov\" strain of virus by the drinking water.", "content": "After a brief reference to the ordinary use of the \"Komarov\" mesogenic vaccinal strain of Newcastle disease virus for the secondary  vaccination of domestic fowls, the authors deal with laboratory experiments conducted to ascertain that this strain can be used for the same purpose by the drinking  water method. Three groups of chickens 45 days old previously vaccinated with LaSota strain in the drinking water at 20 days old, were revaccinated with the normal dose by the \"Komarov\" strain, as follows: a) intramuscularly, b) by the drinking water and c) per os. A fourth group was left as negative control. To investigate the effect of the revaccination by the two different routes of administration, the hemagglutination-inhibition test was used. The results of these tests are finally compared and discussed.", "contents": "Studies on vaccination against Newcastle disease with the \"Komarov\" strain of virus by the drinking water. After a brief reference to the ordinary use of the \"Komarov\" mesogenic vaccinal strain of Newcastle disease virus for the secondary  vaccination of domestic fowls, the authors deal with laboratory experiments conducted to ascertain that this strain can be used for the same purpose by the drinking  water method. Three groups of chickens 45 days old previously vaccinated with LaSota strain in the drinking water at 20 days old, were revaccinated with the normal dose by the \"Komarov\" strain, as follows: a) intramuscularly, b) by the drinking water and c) per os. A fourth group was left as negative control. To investigate the effect of the revaccination by the two different routes of administration, the hemagglutination-inhibition test was used. The results of these tests are finally compared and discussed.", "PMID": 955275}
{"id": "pubmed23n0032_1563", "title": "Antibodies in blood and secretions of chickens immunized parenterally and locally with killed Newcastle disease virus vaccine.", "content": "In mammals the antibody activity in secretions is associated with the secretory IgA. This unique immunoglobulin plays a major role in immunity against infectious diseases of the alimentary and the respiratory tracts. We found a similar system of local immunity against Newcastle disease virus (NDV) in the fowl. Airway washings and bile globulins of chickens were examined by immunodiffusion and immunoelectrophoretic methods. No antigenic differences were found between the serum IgA  and the secretory IgA of airway washings and of bile. The chicken IgA differs from mammalian IgA by lacking secretory component. Antibodies in the airway washings and in tears of chickens immunized by aerosol ir i.m. NDV vaccine (attenuated or inactive) were compared to serum antibodies produced by similar methods of administration. HI antibodies in secretions reached higher levels after aerosol vaccination than after i.m. administration, whereas in serum the situation was reversed. The antibody activity in airway washings and in tears is associated with IgA, while in serum the main antibody is IgG.", "contents": "Antibodies in blood and secretions of chickens immunized parenterally and locally with killed Newcastle disease virus vaccine. In mammals the antibody activity in secretions is associated with the secretory IgA. This unique immunoglobulin plays a major role in immunity against infectious diseases of the alimentary and the respiratory tracts. We found a similar system of local immunity against Newcastle disease virus (NDV) in the fowl. Airway washings and bile globulins of chickens were examined by immunodiffusion and immunoelectrophoretic methods. No antigenic differences were found between the serum IgA  and the secretory IgA of airway washings and of bile. The chicken IgA differs from mammalian IgA by lacking secretory component. Antibodies in the airway washings and in tears of chickens immunized by aerosol ir i.m. NDV vaccine (attenuated or inactive) were compared to serum antibodies produced by similar methods of administration. HI antibodies in secretions reached higher levels after aerosol vaccination than after i.m. administration, whereas in serum the situation was reversed. The antibody activity in airway washings and in tears is associated with IgA, while in serum the main antibody is IgG.", "PMID": 955276}
{"id": "pubmed23n0032_1564", "title": "An interim report on the work of the Avian Products Standardization Committee.", "content": "This Committee was established during the course of the IABS International Symposium on Requirements for Poultry Virus Vaccines held in conjunction with the Foundation M\u00e9rieux at Lyon, France, from 28-30 August, 1973. The sub-committees appointed by this Committee have now submitted resports of their individual activities and it is the purpose of this communication to present in summary form the work which they have so far accomplished.", "contents": "An interim report on the work of the Avian Products Standardization Committee. This Committee was established during the course of the IABS International Symposium on Requirements for Poultry Virus Vaccines held in conjunction with the Foundation M\u00e9rieux at Lyon, France, from 28-30 August, 1973. The sub-committees appointed by this Committee have now submitted resports of their individual activities and it is the purpose of this communication to present in summary form the work which they have so far accomplished.", "PMID": 955278}
{"id": "pubmed23n0032_1565", "title": "The oral rabies immunization of foxes and dogs with sausage baits.", "content": "Foxes wer immunized orally with an attenuated rabies vaccine, ERA, grown on BHK cells. The liquid vaccine was placed in plastic straws, which in turn were incorporated into smoked sausage baits, acceptable to and readily ingested by the animals. When the baits were bitten and the meat swallowed, an oral immunizing dose of vaccine resulted in circulating antibody titers in foxes (and dogs); the animals with antibody resisted a \"street\" rabies virus challenge that killed unvaccinated controls. The immunization was strictly lingual and buccal, and foxes with interrupted esophagi developed antibody only if the vaccine was deposited in the mouth, while those given a similar dose in the ventral esophagostomy opening (below the interruption and close to the stomach) failed to develop antibody. A casein hydrolysate derivative resulted in such stabilization of the liquid that even when baits were held at 35 degree C for 3 days, similar to extreme field conditions, an immunizing titer for foxes (greater than or equal to 10(4.5)LD50) was still maintained.", "contents": "The oral rabies immunization of foxes and dogs with sausage baits. Foxes wer immunized orally with an attenuated rabies vaccine, ERA, grown on BHK cells. The liquid vaccine was placed in plastic straws, which in turn were incorporated into smoked sausage baits, acceptable to and readily ingested by the animals. When the baits were bitten and the meat swallowed, an oral immunizing dose of vaccine resulted in circulating antibody titers in foxes (and dogs); the animals with antibody resisted a \"street\" rabies virus challenge that killed unvaccinated controls. The immunization was strictly lingual and buccal, and foxes with interrupted esophagi developed antibody only if the vaccine was deposited in the mouth, while those given a similar dose in the ventral esophagostomy opening (below the interruption and close to the stomach) failed to develop antibody. A casein hydrolysate derivative resulted in such stabilization of the liquid that even when baits were held at 35 degree C for 3 days, similar to extreme field conditions, an immunizing titer for foxes (greater than or equal to 10(4.5)LD50) was still maintained.", "PMID": 955279}
{"id": "pubmed23n0032_1566", "title": "Experiments with a model of aerosol immunization of mice and swine against Erysipelothrix insidiosa (E. i.).", "content": "Whole antigens  as well as antigen fractions from E.i. were tested as aerosol vaccines. The aerosol produced by a jet nebulizer (Pari, Standard 088) with a capacity of 15 1 air per min and about 10g vaccine per hour was tested by size measuring of the aerosol particles and bacteriological evidence of E.i. in lung tissue. The main concern of the studies was to test the immunogenicity for laboratory animals which were exposed to the vaccines in a modified Henderson apparatus or in boxes of 30 1 respectively. Vaccination trials with conventional mice and piglets did not produce 100% protection against challenge infection by aerosol or subcutaneous route. Experiments with gnotobiotic piglets resulted in sufficient immunity against aerosol challenge with 100 LD50  when aerosols with living avirulent bacteria (strain B 10) were applied three times for one hour or once during three hours. A commercial vaccine from lyzed bacteria immunized only when aerosols were applied three times for three hours. For further immunological evaluation the sera of piglets were tested by means of an indirect fluorescent technique and by other serological methods.", "contents": "Experiments with a model of aerosol immunization of mice and swine against Erysipelothrix insidiosa (E. i.). Whole antigens  as well as antigen fractions from E.i. were tested as aerosol vaccines. The aerosol produced by a jet nebulizer (Pari, Standard 088) with a capacity of 15 1 air per min and about 10g vaccine per hour was tested by size measuring of the aerosol particles and bacteriological evidence of E.i. in lung tissue. The main concern of the studies was to test the immunogenicity for laboratory animals which were exposed to the vaccines in a modified Henderson apparatus or in boxes of 30 1 respectively. Vaccination trials with conventional mice and piglets did not produce 100% protection against challenge infection by aerosol or subcutaneous route. Experiments with gnotobiotic piglets resulted in sufficient immunity against aerosol challenge with 100 LD50  when aerosols with living avirulent bacteria (strain B 10) were applied three times for one hour or once during three hours. A commercial vaccine from lyzed bacteria immunized only when aerosols were applied three times for three hours. For further immunological evaluation the sera of piglets were tested by means of an indirect fluorescent technique and by other serological methods.", "PMID": 955280}
{"id": "pubmed23n0032_1567", "title": "Oral immunization against tetanus.", "content": "Model experiments in mice were used for the investigation of oral immunization against tetanus. The efficacy of oral tetanus toxoid application was tested by subcutaneous challenge (10 LD50 tetanus toxin) and antitoxin determination by L+-method. Reasonable protection could only be achieved when toxoid was in intensive contact with the mucous membranes of the oral cavity, whereas immunization by way of the gastrointestinal tract failed completely. After a single oral vaccination with 200 Lf fluid tetanus toxoid onto mucous membranes of the oral  cavity the efficiency index was 75.  A 100% protection was obtained in challenge after 3 applications of 200 Lf toxoid. In comparison, a single subcutaneous dose of 2 Lf tetanus toxoid resulted in an efficiency index of 100 and a  single intranasal immunization with 100 Lf in an efficiency index of 93. Development of immunity after oral immunization was much accelerated if compared with parenteral immunization. 20% of the orally treated animals were immune  5 days after vaccination. After the same period subcutaneously treated animals had failed to develop any protection at all. Antitoxin titers after one oral vaccination (200 Lf) were similar to the ones after one subcutaneous dose (2 Lf). A distinct increase in antitoxin titers could only be observed when revaccination was done subcutaneously. The results obtained with mice apply analogically to guinea pigs.", "contents": "Oral immunization against tetanus. Model experiments in mice were used for the investigation of oral immunization against tetanus. The efficacy of oral tetanus toxoid application was tested by subcutaneous challenge (10 LD50 tetanus toxin) and antitoxin determination by L+-method. Reasonable protection could only be achieved when toxoid was in intensive contact with the mucous membranes of the oral cavity, whereas immunization by way of the gastrointestinal tract failed completely. After a single oral vaccination with 200 Lf fluid tetanus toxoid onto mucous membranes of the oral  cavity the efficiency index was 75.  A 100% protection was obtained in challenge after 3 applications of 200 Lf toxoid. In comparison, a single subcutaneous dose of 2 Lf tetanus toxoid resulted in an efficiency index of 100 and a  single intranasal immunization with 100 Lf in an efficiency index of 93. Development of immunity after oral immunization was much accelerated if compared with parenteral immunization. 20% of the orally treated animals were immune  5 days after vaccination. After the same period subcutaneously treated animals had failed to develop any protection at all. Antitoxin titers after one oral vaccination (200 Lf) were similar to the ones after one subcutaneous dose (2 Lf). A distinct increase in antitoxin titers could only be observed when revaccination was done subcutaneously. The results obtained with mice apply analogically to guinea pigs.", "PMID": 955281}
{"id": "pubmed23n0032_1568", "title": "Active avoidance and brain DNA after postnatal food deprivation in rats.", "content": "Fond deprivation was produced in infant rats, from 2 to 20 days of age, by a procedure designed to minimize reduction in maternal care. Body weights in the deprived animals were 60% of control weights at 7 days, 65% at 20 days, and, after ad lib feeding, 89% at 4 months. No change was observed in the rate of acquistion of a conditioned active avoidance response in the food-deprived rats when compared with their littermate controls when tested as adults. No lasting reduction in cerebral desoxynucleic acid (DNA) was observed despote a 9% reduction in cerebellar DNA.", "contents": "Active avoidance and brain DNA after postnatal food deprivation in rats. Fond deprivation was produced in infant rats, from 2 to 20 days of age, by a procedure designed to minimize reduction in maternal care. Body weights in the deprived animals were 60% of control weights at 7 days, 65% at 20 days, and, after ad lib feeding, 89% at 4 months. No change was observed in the rate of acquistion of a conditioned active avoidance response in the food-deprived rats when compared with their littermate controls when tested as adults. No lasting reduction in cerebral desoxynucleic acid (DNA) was observed despote a 9% reduction in cerebellar DNA.", "PMID": 955282}
{"id": "pubmed23n0032_1569", "title": "Depth perception after infant and adult visual neocortical lesions in light- and dark-reared rats.", "content": "In 2 experiments the behavior of light- and dark-reared infant- and adult-operated striate rats were compared at 20-160 days of age on a visual cliff apparatus in which the depth of the deep side could be varied. Differential depth thresholds revealed that depth discriminative ability did not develop normally following removal of the striate cortex in infancy. Further, infant-operates who were reared in darkness following their operations performed less well than their light-reared, infant-operated counterparts. The infant-operated animals, regardless of their postoperative rearing condition, performed significantly better than did adult-operated animals after comparable post-operative recovery periods and testing. The results are discussed in terms of further specification of the role played by age of operation and by the interaction between visual experience and age of operation.", "contents": "Depth perception after infant and adult visual neocortical lesions in light- and dark-reared rats. In 2 experiments the behavior of light- and dark-reared infant- and adult-operated striate rats were compared at 20-160 days of age on a visual cliff apparatus in which the depth of the deep side could be varied. Differential depth thresholds revealed that depth discriminative ability did not develop normally following removal of the striate cortex in infancy. Further, infant-operates who were reared in darkness following their operations performed less well than their light-reared, infant-operated counterparts. The infant-operated animals, regardless of their postoperative rearing condition, performed significantly better than did adult-operated animals after comparable post-operative recovery periods and testing. The results are discussed in terms of further specification of the role played by age of operation and by the interaction between visual experience and age of operation.", "PMID": 955283}
{"id": "pubmed23n0032_1570", "title": "Effects of differential cue availability in an active avoidance CS for young and adult rats.", "content": "In Experiment I, groups of 22-and 140+-day rats were trained in acquisition and extinction of 1-way avoidance with a CS that consisted of the opening of a guilotine door 5 sec before US onset or a combination of door-opening plus a tonal signal that remained on until the occurrence of the motor response. Under both CS conditions, avoidance acquistion was similar at each age level. The extinction date indicated comparable performance for the young subjects but differential performance and greater resistance to extinction for the adult subjects. Adults trained with the door-opening CS persisted in responding for an entire series of 100 extinction trials, whereas the adults trained with the compound CS extinguished well within the 100-trial, whereas the adults trained with the compound CS extinguished well within the 100-trial limit. A 2nd experiment included 10 pre-exposures of the simple or compound CS's prior to avoidance training. Although the pups were insensitive to pre-exposure effects, the adults that were pre-exposed and trained with identical CS's showed evidence of pre-exposure effects. Results of both experiments were interpreted as indicative of differential cue saliency between ages.", "contents": "Effects of differential cue availability in an active avoidance CS for young and adult rats. In Experiment I, groups of 22-and 140+-day rats were trained in acquisition and extinction of 1-way avoidance with a CS that consisted of the opening of a guilotine door 5 sec before US onset or a combination of door-opening plus a tonal signal that remained on until the occurrence of the motor response. Under both CS conditions, avoidance acquistion was similar at each age level. The extinction date indicated comparable performance for the young subjects but differential performance and greater resistance to extinction for the adult subjects. Adults trained with the door-opening CS persisted in responding for an entire series of 100 extinction trials, whereas the adults trained with the compound CS extinguished well within the 100-trial, whereas the adults trained with the compound CS extinguished well within the 100-trial limit. A 2nd experiment included 10 pre-exposures of the simple or compound CS's prior to avoidance training. Although the pups were insensitive to pre-exposure effects, the adults that were pre-exposed and trained with identical CS's showed evidence of pre-exposure effects. Results of both experiments were interpreted as indicative of differential cue saliency between ages.", "PMID": 955284}
{"id": "pubmed23n0032_1571", "title": "Scopolamine's effect on passive avoidance behavior in immature rats.", "content": "Male albion rats ranging in age from 15-30 days were injected with either scopolamine hydorbromide or saline, prior to training and retention testing on a black-white passive avoidance (PA) task. Pretraining administration of a 1.0-mg/kg dose of scopolamine significantly increased the median number of trails to criterion for 18-, 21-, and 30-day-old rat pups when compared with their saline controls. Fifteen-day-olds showed drug-related PA deficits when a 2.0-mg/kg dose was given. Retention data reflect characteristic age-dependent memory loss over the 1-week acquistionretention period with no apparent state-dependent effects. The data suggest the presence of cholinergic inhibitory mediation of PA responding in preweanling and postweanling pups.", "contents": "Scopolamine's effect on passive avoidance behavior in immature rats. Male albion rats ranging in age from 15-30 days were injected with either scopolamine hydorbromide or saline, prior to training and retention testing on a black-white passive avoidance (PA) task. Pretraining administration of a 1.0-mg/kg dose of scopolamine significantly increased the median number of trails to criterion for 18-, 21-, and 30-day-old rat pups when compared with their saline controls. Fifteen-day-olds showed drug-related PA deficits when a 2.0-mg/kg dose was given. Retention data reflect characteristic age-dependent memory loss over the 1-week acquistionretention period with no apparent state-dependent effects. The data suggest the presence of cholinergic inhibitory mediation of PA responding in preweanling and postweanling pups.", "PMID": 955285}
{"id": "pubmed23n0032_1572", "title": "The influence of early auditory experience on later auditory and tactual variation seeking in the rat.", "content": "Albino rats were raised under 1 of 4 conditions of auditory stimulation from 22-35 days of age. At 40 days, preferences for auditory and tactual variation were assessed in separate runway tests. Results were analyzed in a split-plot design for each of 2 measures of variation seeking. Subjects experiencing more-variable early stimulation scored higher than subjects receiving less-variable stimulation on 1 measure of variation-seeking and lower on the 2nd measure. The effect was intermodal since these results were obtained for tactual as well as auditory variation seeking.", "contents": "The influence of early auditory experience on later auditory and tactual variation seeking in the rat. Albino rats were raised under 1 of 4 conditions of auditory stimulation from 22-35 days of age. At 40 days, preferences for auditory and tactual variation were assessed in separate runway tests. Results were analyzed in a split-plot design for each of 2 measures of variation seeking. Subjects experiencing more-variable early stimulation scored higher than subjects receiving less-variable stimulation on 1 measure of variation-seeking and lower on the 2nd measure. The effect was intermodal since these results were obtained for tactual as well as auditory variation seeking.", "PMID": 955286}
{"id": "pubmed23n0032_1573", "title": "A comparison of live and videorecord viewing of infant behavior under sound stimulation. III. Six-month-old babies.", "content": "Ten clinically normal 6-month-old infants were presented with a series of sound stimuli while their behavior was simultaneously videotaperecorded and assessed live by 2 sound-masked observers. Two weeks later the same observers assessed the behavior from the videorecords. In both situations observers were permitted to see the babies for 13 sec at each trial. The first 5 sec formed a prestimulus observation period and the following 8 sec possibly contained a sound stimulus. Between trials the observers were allowed 20 sec in which to not (1) prestimulus activity, (2) confidence in response, and (3) facial, digit, and limb movements and \"wholistic\"impressions (e.g., \"stilling\"). The agreement between the live and video situations was modest for prestimulus activity and confidence in response, and fair to good for movement item. The outcome was rather similar to that of our earlier work on neonates and 6-week infants, although it did suggest a small loss of information with the videorecording of 6-month infants.", "contents": "A comparison of live and videorecord viewing of infant behavior under sound stimulation. III. Six-month-old babies. Ten clinically normal 6-month-old infants were presented with a series of sound stimuli while their behavior was simultaneously videotaperecorded and assessed live by 2 sound-masked observers. Two weeks later the same observers assessed the behavior from the videorecords. In both situations observers were permitted to see the babies for 13 sec at each trial. The first 5 sec formed a prestimulus observation period and the following 8 sec possibly contained a sound stimulus. Between trials the observers were allowed 20 sec in which to not (1) prestimulus activity, (2) confidence in response, and (3) facial, digit, and limb movements and \"wholistic\"impressions (e.g., \"stilling\"). The agreement between the live and video situations was modest for prestimulus activity and confidence in response, and fair to good for movement item. The outcome was rather similar to that of our earlier work on neonates and 6-week infants, although it did suggest a small loss of information with the videorecording of 6-month infants.", "PMID": 955287}
{"id": "pubmed23n0032_1574", "title": "The stability and interrelationships of newborn sucking and heart rate.", "content": "The sucking behavior of 44 newborns was recorded along with heart rate (HR) and respiration. These 3 systems showed stability over a 24-hr period. Sucking parameters varied markedly depending upon whether the infant was sucking for sucrose or under a no-fluid condition. Moreover, HR was strikingly affected by sweetness. The direction of HR change was toward increasing rates when sucking for sweet, even though sucking for sweet substances occurs more slowly than for no fluid.", "contents": "The stability and interrelationships of newborn sucking and heart rate. The sucking behavior of 44 newborns was recorded along with heart rate (HR) and respiration. These 3 systems showed stability over a 24-hr period. Sucking parameters varied markedly depending upon whether the infant was sucking for sucrose or under a no-fluid condition. Moreover, HR was strikingly affected by sweetness. The direction of HR change was toward increasing rates when sucking for sweet, even though sucking for sweet substances occurs more slowly than for no fluid.", "PMID": 955288}
{"id": "pubmed23n0032_1575", "title": "Position reversal deficit in young ferrets.", "content": "Young ferrets (6 and 10 weeks old at the start of the experiments) were compared to adult ferrets on learning (1) a Lashley III maze, (2) a left-right discrimination in T-maze with identical choice arms, and (3) a reversal of the left-right discrimination. The young were not inferior to the adults in learning either the Lashley III maze or the initial discrimination in the T-maze. However, the young were markedly (and significantly) inferior to the adults in learning to reverse.", "contents": "Position reversal deficit in young ferrets. Young ferrets (6 and 10 weeks old at the start of the experiments) were compared to adult ferrets on learning (1) a Lashley III maze, (2) a left-right discrimination in T-maze with identical choice arms, and (3) a reversal of the left-right discrimination. The young were not inferior to the adults in learning either the Lashley III maze or the initial discrimination in the T-maze. However, the young were markedly (and significantly) inferior to the adults in learning to reverse.", "PMID": 955289}
{"id": "pubmed23n0032_1576", "title": "Behavioral development in three hamster species.", "content": "The preweaning behavioral development was compared in 3 hamster species: the golden (Mesocricetus auratus), Chinese (Cricetulus griseus), and Djungarian (Phodopus sungorus). Differences in the age of appearance of action patterns could not be summarized by simply ranking species on either precocity or developmental speed, nor were they eliminated by measuring age from conception. Problems in the interpretation of species differences in the age of appearance of action patterns are discussed.", "contents": "Behavioral development in three hamster species. The preweaning behavioral development was compared in 3 hamster species: the golden (Mesocricetus auratus), Chinese (Cricetulus griseus), and Djungarian (Phodopus sungorus). Differences in the age of appearance of action patterns could not be summarized by simply ranking species on either precocity or developmental speed, nor were they eliminated by measuring age from conception. Problems in the interpretation of species differences in the age of appearance of action patterns are discussed.", "PMID": 955290}
{"id": "pubmed23n0032_1577", "title": "The effect of reinstatement stimulus conditions on the maintenance of long-term memory.", "content": "Weanling rats trained on an appetitive SDSdelta discrimination were subsequently exposed to different components of the training stimulus for 15 min each week for 10 successive weeks. On the week following the final stimulus exposure retention was measured by a 2-hr relearning test. Those subjects exposed to all acquisition cues (SDSdelta with reinforcement), as well as those exposed to Sdelta conditions alone, demonstrated enhanced retention relative to a control group receiving no reinstatement experience. All other exposure conditions including presentation of SD with reinforecement were either ineffective or impaired retention.", "contents": "The effect of reinstatement stimulus conditions on the maintenance of long-term memory. Weanling rats trained on an appetitive SDSdelta discrimination were subsequently exposed to different components of the training stimulus for 15 min each week for 10 successive weeks. On the week following the final stimulus exposure retention was measured by a 2-hr relearning test. Those subjects exposed to all acquisition cues (SDSdelta with reinforcement), as well as those exposed to Sdelta conditions alone, demonstrated enhanced retention relative to a control group receiving no reinstatement experience. All other exposure conditions including presentation of SD with reinforecement were either ineffective or impaired retention.", "PMID": 955291}
{"id": "pubmed23n0032_1578", "title": "Motor reactions during the antenatal period correlated with the periodic change in the activity of the cardiovascular system.", "content": "A connection between the periodic appearance of vagal bradycardia and of intrauterine large-amplitude respiratory movements, as well as the appearance of generalized jerky-type and more sustained extensor motor reactions, has been established in experiments on dog fetuses connected with the mother through the umbilical cord. Brief vagal bradycardia also arises immediately after completion of these reactions and is an analogue of the vagal phase in adults after motor activity. These correlated phenomena are caused by periodic reversible mild hypoxemia which through the chemoreceptors of the sinocarotid zone stimulates the centers of vagal innervation of the heart and the centers of innervation of skeletal muscles. These phenomena disappear when the mother inhales a gas mixture containing less than 14-15% oxygen or greater than 50-60% oxygen, as well as when the mother is under deep anesthesia.", "contents": "Motor reactions during the antenatal period correlated with the periodic change in the activity of the cardiovascular system. A connection between the periodic appearance of vagal bradycardia and of intrauterine large-amplitude respiratory movements, as well as the appearance of generalized jerky-type and more sustained extensor motor reactions, has been established in experiments on dog fetuses connected with the mother through the umbilical cord. Brief vagal bradycardia also arises immediately after completion of these reactions and is an analogue of the vagal phase in adults after motor activity. These correlated phenomena are caused by periodic reversible mild hypoxemia which through the chemoreceptors of the sinocarotid zone stimulates the centers of vagal innervation of the heart and the centers of innervation of skeletal muscles. These phenomena disappear when the mother inhales a gas mixture containing less than 14-15% oxygen or greater than 50-60% oxygen, as well as when the mother is under deep anesthesia.", "PMID": 955292}
{"id": "pubmed23n0032_1579", "title": "Stability of motor OR and heart rate habituation rates of infancy.", "content": "The stability of the OR habituation rate as an individual difference trait was investigated in 2 studies. The 1st focused on motor OR's to auditory stimuli at 3 ages within the 1st month of life, and the 2nd examined the HR responses to auditory stimuli at 4 and 8 months. The results indicate that motor OR's did not differ significantly with age and were moderately stable across ages. The stability decreased with age across consecutive days but increased across consecutive weeks. The cross-age correlation for HR responses was moderate. The results were compared with similar findings on other parameters of the OR.", "contents": "Stability of motor OR and heart rate habituation rates of infancy. The stability of the OR habituation rate as an individual difference trait was investigated in 2 studies. The 1st focused on motor OR's to auditory stimuli at 3 ages within the 1st month of life, and the 2nd examined the HR responses to auditory stimuli at 4 and 8 months. The results indicate that motor OR's did not differ significantly with age and were moderately stable across ages. The stability decreased with age across consecutive days but increased across consecutive weeks. The cross-age correlation for HR responses was moderate. The results were compared with similar findings on other parameters of the OR.", "PMID": 955293}
{"id": "pubmed23n0032_1580", "title": "Effects of pre- and postnatal litter size reduction on development and behavior of rat offspring.", "content": "Litter size was reduced to 2-5 rat pups either prenatally by unilateral maternal oviduct ligation (Group PRN) or postnatally by removing pups (Group PST). Normal size litters (8-10 pups) of sham ligated (SHM) and intact (CON) mothers served as controls. Weights at 30 days were increased by prenatal or postnatal reduction and reduced by prenatal stress (SHM); the sex difference in weight was most pronounced in PRN rats. At 75 days PRN rats were heaviest, with no differences between the other groups. Relative ovarian weights were reduced in PRN females and absolute testes weights increased in PST males. The PRN and SHM females had smaller relative adrenal weights than CON and PST females. Open-field activity was generally increased by prior avoidance conditioning and effects of treatments were found only in groups tested after avoidance-conditioning: PRN and SHM rats were more active than PST and CON rats, particularly on Days 1 (SHM) and 4 (SHM and PRN) of testing. Passive-avoidance behavior of PRN rats was also more susceptible to previous test experience: they emerged more slowly if they had prior open-field experience. The PST animals, in contrast, emerged more rapidly after prior test experience. Plasma corticosterone levels and shuttlebox conditioning and extinction were unaffected by treatments.", "contents": "Effects of pre- and postnatal litter size reduction on development and behavior of rat offspring. Litter size was reduced to 2-5 rat pups either prenatally by unilateral maternal oviduct ligation (Group PRN) or postnatally by removing pups (Group PST). Normal size litters (8-10 pups) of sham ligated (SHM) and intact (CON) mothers served as controls. Weights at 30 days were increased by prenatal or postnatal reduction and reduced by prenatal stress (SHM); the sex difference in weight was most pronounced in PRN rats. At 75 days PRN rats were heaviest, with no differences between the other groups. Relative ovarian weights were reduced in PRN females and absolute testes weights increased in PST males. The PRN and SHM females had smaller relative adrenal weights than CON and PST females. Open-field activity was generally increased by prior avoidance conditioning and effects of treatments were found only in groups tested after avoidance-conditioning: PRN and SHM rats were more active than PST and CON rats, particularly on Days 1 (SHM) and 4 (SHM and PRN) of testing. Passive-avoidance behavior of PRN rats was also more susceptible to previous test experience: they emerged more slowly if they had prior open-field experience. The PST animals, in contrast, emerged more rapidly after prior test experience. Plasma corticosterone levels and shuttlebox conditioning and extinction were unaffected by treatments.", "PMID": 955294}
{"id": "pubmed23n0032_1581", "title": "Escape learning in infant mice as a function of drive level and drive shifts during acquisition.", "content": "Separate groups of 9-day-old Swiss-Webster mice began straight-alley escape training at .1 or .4 mA. After 12 trials, half of the mice in each group were shifted to .4 or .1 mA, respectively, whereas the remaining half continued at their original level for an additional 12 trials. Twenty-four hours later, half of each of the 4 shock-level groups were retested at .1 mA, half at .4 mA. The results indicated that those groups which made a large number of competing responses during early trials and showed a gradual reduction over training trials (.1-.1 and .1-.4) emitted the fewest number during retest at either shock level. In contrast, those groups with either limited (.4-.1) or no (.4-.4) opportunity to decrease competing responses during training showed evidence of poor (.4-.1) and no (.4-.4) retention of learned inhibition of that response. Running speed was clearly a performance measure, as it only reflected existing shock levels during both training and retention trials.", "contents": "Escape learning in infant mice as a function of drive level and drive shifts during acquisition. Separate groups of 9-day-old Swiss-Webster mice began straight-alley escape training at .1 or .4 mA. After 12 trials, half of the mice in each group were shifted to .4 or .1 mA, respectively, whereas the remaining half continued at their original level for an additional 12 trials. Twenty-four hours later, half of each of the 4 shock-level groups were retested at .1 mA, half at .4 mA. The results indicated that those groups which made a large number of competing responses during early trials and showed a gradual reduction over training trials (.1-.1 and .1-.4) emitted the fewest number during retest at either shock level. In contrast, those groups with either limited (.4-.1) or no (.4-.4) opportunity to decrease competing responses during training showed evidence of poor (.4-.1) and no (.4-.4) retention of learned inhibition of that response. Running speed was clearly a performance measure, as it only reflected existing shock levels during both training and retention trials.", "PMID": 955295}
{"id": "pubmed23n0032_1582", "title": "Carbohydrate metabolism and capillary basement-membrane thickness in children. I. cross-sectional studies.", "content": "Thirty-nine children from three to 16 years of age were included in this study. Nineteen were diabetics, seven were \"suspected\" diabetics (with evidence of glucose intolerance but without repeated fasting hyperglycemia), and 13 were controls. Mean glucose disappearance rates (K) during intravenous glucose tolerance tests (IVGTTs) were 2.19 for the controls, 1.23 for the suspected diabetics, and 0.70 for 14 diabetics tested; the differences were statistically significant. Diabetics had the smallest and suspected diabetics the greatest plasma insulin responses during IVGTTs. Fasting plasma growth hormone (GH) varied widely. GH generally decreased or remained unchanged during IVGTTs, but two diabetics exhibited slight increases and two sustpected diabetics pronounced increases. Basement-membrane thickness (BMT) was examined in 42 quadriceps femoris needle biopsies. Average BMT (ABMT) and minimum BMT (MBMT) correlated well (r = 0.91). BMT did not correlate well with age or, in the diabetics, with duration of clinical disease. Diabetics had the greatest and controls the least mean ABMT and MBMT, but the differences were not statistically significant. High values (those exceeding mean control values by more than two standard deviations) were much more common among the suspected diabetics and the diabetics. One of 13 controls, three of six suspected diabetics, and six of 19 diabetics had high ABMT values; two suspected diabetics and five diabetics had high MBMT values. Correlations between BMT and K were negative for the most part, but correlation coefficients were small. Serial studies in four of the children suggest that BMT and glucose tolerance tend to change in opposite directions.", "contents": "Carbohydrate metabolism and capillary basement-membrane thickness in children. I. cross-sectional studies. Thirty-nine children from three to 16 years of age were included in this study. Nineteen were diabetics, seven were \"suspected\" diabetics (with evidence of glucose intolerance but without repeated fasting hyperglycemia), and 13 were controls. Mean glucose disappearance rates (K) during intravenous glucose tolerance tests (IVGTTs) were 2.19 for the controls, 1.23 for the suspected diabetics, and 0.70 for 14 diabetics tested; the differences were statistically significant. Diabetics had the smallest and suspected diabetics the greatest plasma insulin responses during IVGTTs. Fasting plasma growth hormone (GH) varied widely. GH generally decreased or remained unchanged during IVGTTs, but two diabetics exhibited slight increases and two sustpected diabetics pronounced increases. Basement-membrane thickness (BMT) was examined in 42 quadriceps femoris needle biopsies. Average BMT (ABMT) and minimum BMT (MBMT) correlated well (r = 0.91). BMT did not correlate well with age or, in the diabetics, with duration of clinical disease. Diabetics had the greatest and controls the least mean ABMT and MBMT, but the differences were not statistically significant. High values (those exceeding mean control values by more than two standard deviations) were much more common among the suspected diabetics and the diabetics. One of 13 controls, three of six suspected diabetics, and six of 19 diabetics had high ABMT values; two suspected diabetics and five diabetics had high MBMT values. Correlations between BMT and K were negative for the most part, but correlation coefficients were small. Serial studies in four of the children suggest that BMT and glucose tolerance tend to change in opposite directions.", "PMID": 955296}
{"id": "pubmed23n0032_1583", "title": "Carbohydrate metabolism and capillary basement-membrane thickness in children. II. Longitudinal studies.", "content": "Longitudinal biochemical and histologic studies were carried out in 11 children receiving oral hypoglycemic agents. There were five \"suspected\" diabetics (with evidence of glucose intolerance but without repeated fasting hyperglycemia) and six diabetics. Mean fasting plasma glucose (FPG) values showed no significant change during treatment with phenformin alone. The mean FPG decreased significantly within six to 10 weeks after addition of tolazamide to the regimen, but the decrease was not sustained during long-term observation (seven months to four years). Glucose disappearance rate (K) generally increased as FPG decreased, but the number of observations was smaller, and mean values showed no significant change. Mean values for fasting plasma insulin and for peak insulin response to intravenously administered glucose did not change significantly. Changes in capillary basement membrane thickness (BMT) were found to be statistically significant in a number of individual instances. Decreasing BMTs were associated with increasing Ks and vice versa. A similar trend was apparent among other patients, in whom individual changes in BMT were not statistically significant. The pooled data were therefore subjected to chi-square analysis; K and average BMT were found to change in opposite directions (P less than 0.001); a similar relationship held for K and minimum BMT (P less than 0.05). But K and BMT did not correlate well as regards magnitude of change. Influences of phenformin and tolazamide on these changes could not be evaluated. Both BMT and K may be influenced by many complex variables, but the present findings indicate that glucose tolerance and BMT have a close interdependence.", "contents": "Carbohydrate metabolism and capillary basement-membrane thickness in children. II. Longitudinal studies. Longitudinal biochemical and histologic studies were carried out in 11 children receiving oral hypoglycemic agents. There were five \"suspected\" diabetics (with evidence of glucose intolerance but without repeated fasting hyperglycemia) and six diabetics. Mean fasting plasma glucose (FPG) values showed no significant change during treatment with phenformin alone. The mean FPG decreased significantly within six to 10 weeks after addition of tolazamide to the regimen, but the decrease was not sustained during long-term observation (seven months to four years). Glucose disappearance rate (K) generally increased as FPG decreased, but the number of observations was smaller, and mean values showed no significant change. Mean values for fasting plasma insulin and for peak insulin response to intravenously administered glucose did not change significantly. Changes in capillary basement membrane thickness (BMT) were found to be statistically significant in a number of individual instances. Decreasing BMTs were associated with increasing Ks and vice versa. A similar trend was apparent among other patients, in whom individual changes in BMT were not statistically significant. The pooled data were therefore subjected to chi-square analysis; K and average BMT were found to change in opposite directions (P less than 0.001); a similar relationship held for K and minimum BMT (P less than 0.05). But K and BMT did not correlate well as regards magnitude of change. Influences of phenformin and tolazamide on these changes could not be evaluated. Both BMT and K may be influenced by many complex variables, but the present findings indicate that glucose tolerance and BMT have a close interdependence.", "PMID": 955297}
{"id": "pubmed23n0032_1584", "title": "Locating the site(s) of insulin resistance in patients with nonketotic diabetes mellitus.", "content": "Insulin resistance and the ability of insulin to inhibit hepatic glucose production and to increas efficiency of glucose uptake were determined in 24 nonobese individuals: eight subjects with normal oral glucose tolerance, eight patients with chemical diabetes, and eight nonketotic patients with fasting hyperglycemia (greater than 150 mg. per cent). Insulin resistance was estimated by measuring the steady-state plasma glucose response to a continuous infusion of insulin, glucose, epinephrine, and propranolol. This approach permits us to inhibit levels of exogenous insulin, and use the height of the steady-state plasma glucose response as a direct estimate of insulin resistance. The ability of insulin to inhibit hepatic glucose production and to increase efficiency of glucose uptake was calculated from the results of two studies in which a continuous infusion of 3H-2-glucose was used to measure glucose turnover rate. The first study was performed after an overnight fast, under conditions of basal insulin levels, while the second was conducted during the infusion of insulin, glucose, epinephrine, and propranolol. Hepatic glucose production is equal to glucose turnover rate during the basal study and is equal to glucose turnover rate minus the infusion rate of cold glucose during the second study. Glucose uptake in both studies is equal to glucose turnover rate minus urinary glucose loss, and the efficiency of glucose uptake is calculated by dividing glucose uptake by the plasma glucose pool size. The mean (+/- S.E.) steady-state plasma glucose response was 113 +/- 9 mg. per cent in normal subjects, 205 +/- 14 mg. per cent in chemical diabetics, and 346 +/- 30 mg. per cent in patients with fasting hyperglycemia. Thus, insulin resistance exists in monoketotic diabetes, and the greater the degree of glucose intolerance, the greater the insulin resistance. The resistance to the insulin infusion in patients with chemical diabetes seemed to be mainly a function of the inability of insulin to increase efficiency of glucose uptake, since insulin did retain its ability to inhibit glucose production (although not to normal levels). In contrast, the infusion of insulin neither inhibited hepatic glucose production nor increased efficienty of glucose uptake in patients with fasting hyperglycemia. Thus, the insulin resistance that exists in patients with nonketotic diabetes cannot be considered to be a global phenomenon. Significant differences exist in the responsiveness of various tissues to the two general aspects of insulin's action on glucose homeostasis, and these differences provide a physiologic basis for the variations in degree of over-all insulin resistance that are present in the three groups of subjects.", "contents": "Locating the site(s) of insulin resistance in patients with nonketotic diabetes mellitus. Insulin resistance and the ability of insulin to inhibit hepatic glucose production and to increas efficiency of glucose uptake were determined in 24 nonobese individuals: eight subjects with normal oral glucose tolerance, eight patients with chemical diabetes, and eight nonketotic patients with fasting hyperglycemia (greater than 150 mg. per cent). Insulin resistance was estimated by measuring the steady-state plasma glucose response to a continuous infusion of insulin, glucose, epinephrine, and propranolol. This approach permits us to inhibit levels of exogenous insulin, and use the height of the steady-state plasma glucose response as a direct estimate of insulin resistance. The ability of insulin to inhibit hepatic glucose production and to increase efficiency of glucose uptake was calculated from the results of two studies in which a continuous infusion of 3H-2-glucose was used to measure glucose turnover rate. The first study was performed after an overnight fast, under conditions of basal insulin levels, while the second was conducted during the infusion of insulin, glucose, epinephrine, and propranolol. Hepatic glucose production is equal to glucose turnover rate during the basal study and is equal to glucose turnover rate minus the infusion rate of cold glucose during the second study. Glucose uptake in both studies is equal to glucose turnover rate minus urinary glucose loss, and the efficiency of glucose uptake is calculated by dividing glucose uptake by the plasma glucose pool size. The mean (+/- S.E.) steady-state plasma glucose response was 113 +/- 9 mg. per cent in normal subjects, 205 +/- 14 mg. per cent in chemical diabetics, and 346 +/- 30 mg. per cent in patients with fasting hyperglycemia. Thus, insulin resistance exists in monoketotic diabetes, and the greater the degree of glucose intolerance, the greater the insulin resistance. The resistance to the insulin infusion in patients with chemical diabetes seemed to be mainly a function of the inability of insulin to increase efficiency of glucose uptake, since insulin did retain its ability to inhibit glucose production (although not to normal levels). In contrast, the infusion of insulin neither inhibited hepatic glucose production nor increased efficienty of glucose uptake in patients with fasting hyperglycemia. Thus, the insulin resistance that exists in patients with nonketotic diabetes cannot be considered to be a global phenomenon. Significant differences exist in the responsiveness of various tissues to the two general aspects of insulin's action on glucose homeostasis, and these differences provide a physiologic basis for the variations in degree of over-all insulin resistance that are present in the three groups of subjects.", "PMID": 955298}
{"id": "pubmed23n0032_1585", "title": "Nodular intercapillary glomeruloscerosis in diabetes secondary to chronic calcific pancreatitis.", "content": "Nodular (specific) intercapillary glomerulosclerosis (Kimmelstiel-Wilson) was found at autopsy in a 47-year-old man who had been diabetic for 20 years. The family history for this disease had been negative. Both the clinical course and the autopsy findings strongly suggest that this patient's diabetes was secondary to chronic fibrocalcific pancreatitis. This is only the fourth recorded case of histologically documented nodular glomerulosclerosis occurring in a patient with pancreatogenic diabetes.", "contents": "Nodular intercapillary glomeruloscerosis in diabetes secondary to chronic calcific pancreatitis. Nodular (specific) intercapillary glomerulosclerosis (Kimmelstiel-Wilson) was found at autopsy in a 47-year-old man who had been diabetic for 20 years. The family history for this disease had been negative. Both the clinical course and the autopsy findings strongly suggest that this patient's diabetes was secondary to chronic fibrocalcific pancreatitis. This is only the fourth recorded case of histologically documented nodular glomerulosclerosis occurring in a patient with pancreatogenic diabetes.", "PMID": 955299}
{"id": "pubmed23n0032_1586", "title": "Is cigarette smoking more frequent among insulin-treated diabetics?", "content": "During epidemiologic investigations of vascular complications of diabetes in Warsaw the habit of cigarette smoking was analyzed in the subjects studied (4,530 diabetics). It was found that in the group of diabetics the number of ex-smokers was much greater (p less than 0.01) and that of present smokers lower (p less tan 0.01) than in a representative sample of the Warsaw population. Furthermore, it was observed that in the subgroup of insulin-treated diabetics the number of smokers was highest and the number of ex-smokers and nonsmokers was lowest in relations to other treatment subgroups.", "contents": "Is cigarette smoking more frequent among insulin-treated diabetics? During epidemiologic investigations of vascular complications of diabetes in Warsaw the habit of cigarette smoking was analyzed in the subjects studied (4,530 diabetics). It was found that in the group of diabetics the number of ex-smokers was much greater (p less than 0.01) and that of present smokers lower (p less tan 0.01) than in a representative sample of the Warsaw population. Furthermore, it was observed that in the subgroup of insulin-treated diabetics the number of smokers was highest and the number of ex-smokers and nonsmokers was lowest in relations to other treatment subgroups.", "PMID": 955300}
{"id": "pubmed23n0032_1587", "title": "Plasma glucose and insulin responses to orally administered simple and complex carbohydrates.", "content": "We have studied the effects of glucose, sucrose, and various starches on postprandial plasma glucose and insulin responses in 19 subjects. All carbohydrate loads were calculated to contain 50 gm. of glucose, and the response to each carbohydrate was tested twice: when given alone in a drink or when given in combination with other nutrients as a meal. The data demonstrate: (1) Glucose and sucrose elicited similar plasma glucose response curves, but sucrose elicited a somewhat greater (20 per cent) plasma insulin response. (2) Raw starch ingestion resulted in a 44 per cent lower glucose response and a 35-65 per cent lower insulin response than did either glucose or sucrose ingestion. (3) When carbohydrate was given as a meal the plasma glucose responses were 40-60 per cent lower than when the same carbohydrate was given as a drink, while the insulin responses were generally similar, and (4) when different cooked starches were compared, the plasma glucose and insulin responses to rice were significantly lower (50 per cent) than to potato. In conclusion, the size of the carbohydrate molecule appears to influence the postprandial glucose and insulin responses such that more complex carbohydrates (starches) elicit lower responses. This effect may be related to differences in digestion rather than to differences in absorption.", "contents": "Plasma glucose and insulin responses to orally administered simple and complex carbohydrates. We have studied the effects of glucose, sucrose, and various starches on postprandial plasma glucose and insulin responses in 19 subjects. All carbohydrate loads were calculated to contain 50 gm. of glucose, and the response to each carbohydrate was tested twice: when given alone in a drink or when given in combination with other nutrients as a meal. The data demonstrate: (1) Glucose and sucrose elicited similar plasma glucose response curves, but sucrose elicited a somewhat greater (20 per cent) plasma insulin response. (2) Raw starch ingestion resulted in a 44 per cent lower glucose response and a 35-65 per cent lower insulin response than did either glucose or sucrose ingestion. (3) When carbohydrate was given as a meal the plasma glucose responses were 40-60 per cent lower than when the same carbohydrate was given as a drink, while the insulin responses were generally similar, and (4) when different cooked starches were compared, the plasma glucose and insulin responses to rice were significantly lower (50 per cent) than to potato. In conclusion, the size of the carbohydrate molecule appears to influence the postprandial glucose and insulin responses such that more complex carbohydrates (starches) elicit lower responses. This effect may be related to differences in digestion rather than to differences in absorption.", "PMID": 955301}
{"id": "pubmed23n0032_1588", "title": "Total cardiac denervation in diabetic autonomic neuropathy.", "content": "Total loss of the autonomic regulation of heart rate is described in a 28-year-old diabetic with extensive autonomic neuropathy. The patient had an almost fixed heart rate that barely responded to any of the tests that stimulate or inhibit the autonomic nerves. Its behavior was similar to that of the transplanted heart.", "contents": "Total cardiac denervation in diabetic autonomic neuropathy. Total loss of the autonomic regulation of heart rate is described in a 28-year-old diabetic with extensive autonomic neuropathy. The patient had an almost fixed heart rate that barely responded to any of the tests that stimulate or inhibit the autonomic nerves. Its behavior was similar to that of the transplanted heart.", "PMID": 955302}
{"id": "pubmed23n0032_1589", "title": "Effect of ethanol on the glucose-mediated insulin release in triply catheterized anesthetized pigs.", "content": "In order to further elucidate the potentiating effect of ethanol on the glucose-mediated insulin response, triply catheterized anesthetized pigs were submitted to an intravenous glucose infusion test after a four-hour preinfusion with ethanol (11 pigs) or saline (six pigs, control experiment). During the tests portal, hepatic, and peripheral venous blood was drawn simultaneously. Two series of ethanol-preinfusion experiments were carried out: in one series the serum ethanol concentration was maintained at approximately 10 mM and in the other at 19 mM. Compared with saline, 10 mM of serum ethanol induced a more than threefold increment in the insulin secretory response to glucose as estimated in the portal blood (p less than 0.01). Likewise, hepatic and peripheral venous blood insulin levels were enhanced (p less than 0.01). In contrast, 19 mM of serum ethanol did not elevate serum insulin levels above those found in the control experiments. When individual incremental portal insulin areas were plotted against the corresponding average value of serum ethanol in the preinfusion period, a significant inverse relationship was found (p less than 0.02), indicating a decrease in the potentiating effect of ethanol on the glucose-mediated insulin response with increasing levels of serum ethanol. Comparison of portal and hepatic incremental insulin areas revealed that ethanol did not, in the concentration range investigated, influence the hepatic insulin degradation rate. In conclusion, ethanol seems to potentiate, in an inverse concentration-dependent manner, the glucose-mediated insulin response through an action directly on the pancreas.", "contents": "Effect of ethanol on the glucose-mediated insulin release in triply catheterized anesthetized pigs. In order to further elucidate the potentiating effect of ethanol on the glucose-mediated insulin response, triply catheterized anesthetized pigs were submitted to an intravenous glucose infusion test after a four-hour preinfusion with ethanol (11 pigs) or saline (six pigs, control experiment). During the tests portal, hepatic, and peripheral venous blood was drawn simultaneously. Two series of ethanol-preinfusion experiments were carried out: in one series the serum ethanol concentration was maintained at approximately 10 mM and in the other at 19 mM. Compared with saline, 10 mM of serum ethanol induced a more than threefold increment in the insulin secretory response to glucose as estimated in the portal blood (p less than 0.01). Likewise, hepatic and peripheral venous blood insulin levels were enhanced (p less than 0.01). In contrast, 19 mM of serum ethanol did not elevate serum insulin levels above those found in the control experiments. When individual incremental portal insulin areas were plotted against the corresponding average value of serum ethanol in the preinfusion period, a significant inverse relationship was found (p less than 0.02), indicating a decrease in the potentiating effect of ethanol on the glucose-mediated insulin response with increasing levels of serum ethanol. Comparison of portal and hepatic incremental insulin areas revealed that ethanol did not, in the concentration range investigated, influence the hepatic insulin degradation rate. In conclusion, ethanol seems to potentiate, in an inverse concentration-dependent manner, the glucose-mediated insulin response through an action directly on the pancreas.", "PMID": 955303}
{"id": "pubmed23n0032_1590", "title": "Insulin and brain metabolism. Absence of direct action of insulin on K+ and Na+ transport in mouse brain.", "content": "This is a study of the effect of insulin on the transport of K+ and Na+ from the blood into the brains of normal mice. Despite profound reductions in plasma and brain glucose levels, reduction of plasma K+ concentration and progressive deterioration of neurologic function 30-120 minutes after insulin injection, in 20-22-day-old animals there was no increase in brain K+ and Na+ concentrations. In fact, at 120 minutes, when the brain water content increased 0.7 per cent, brain K+ concentration was significantly reduced, not elevated. The effect of insulin on brain electrolyte and water content in adult mice was also studied. Although brain water increased 0.5 per cent at 120 minutes, there was no changes in brain Na+ or K+ concentrations at any time after insulin injection. The data from mice do not support a role of insulin in electrolyte transport in brain.", "contents": "Insulin and brain metabolism. Absence of direct action of insulin on K+ and Na+ transport in mouse brain. This is a study of the effect of insulin on the transport of K+ and Na+ from the blood into the brains of normal mice. Despite profound reductions in plasma and brain glucose levels, reduction of plasma K+ concentration and progressive deterioration of neurologic function 30-120 minutes after insulin injection, in 20-22-day-old animals there was no increase in brain K+ and Na+ concentrations. In fact, at 120 minutes, when the brain water content increased 0.7 per cent, brain K+ concentration was significantly reduced, not elevated. The effect of insulin on brain electrolyte and water content in adult mice was also studied. Although brain water increased 0.5 per cent at 120 minutes, there was no changes in brain Na+ or K+ concentrations at any time after insulin injection. The data from mice do not support a role of insulin in electrolyte transport in brain.", "PMID": 955304}
{"id": "pubmed23n0032_1591", "title": "Effect of diabetes mellitus and insulin on the turnover and metabolic response to ketones in man.", "content": "To determine the effect of diabetes mellitus on ketone removal rates, Na DL-beta-hydroxybutyrate was administered as a continuous three-hour infusion (3 mg./kg./min.) to healthy volunteers and insulin-dependent diabetics in the postabsorptive state. An additional group of healthy controls received intravenous glucose (50 gm.) or glucose and insulin during the ketone infusion to determine the effect of hyperinsulinemia on ketone removal. Following ketone infusion, total blood ketone levels in the diabetics were twofold greater than in controls (p less than 0.001). The metabolic clearance rate of ketones (MCRk) in the diabetics was reduced by 42% from that of controls (p less than 0.001). In contrast, the calculated production rate of ketones (PRk) in diabetics was not consistently different from that observed in controls. In diabetics with normal PRk, MCRk remained significantly below control values (p less than 0.001). The ketone infusion resulted in a fall in plasma glucose and alanine levels in the normals as well as diabetics. However, the decline in plasma glucose induced by the ketone infusion was five- to sixfold greater in the diabetics than in controls (p less than 0.005) and correlated linearly with the decline in plasma alanine (p less than 0.02). Administration of intravenous glucose during an ongoing ketone infusion in normal subjects resulted in 37 +/- 5% reduction in beta-hydroxybutyrate, but no change in acetoacetate concentration. The decline in beta-hydroxybutyrate was two- to threefold greater than would be expected if glucose had acted solely to inhibit endogenous ketone production. Similar results were observed when hyperinsulinemia without hyperglycemia was produced by simultaneous administration of insulin and glucose. It is concluded that (1) ketone disposal is reduced in diabetes even when ketone production is normal, suggesting the rate of ketone utilization may be a more sensitive index of insulin deficiency than is ketone production; (2) hyperinsulinemia stimulates beta-hydroxybutyrate utilization without influencing acetoacetate concentration; and (3) increased blood ketone levels induced by infusion of Na DL-beta-hydroxybutyrate reduce plasma glucose and alanine concentrations in diabetes. These findings thus support a role for insulin in influencing ketone disposal in normal as well as diabetic man and a role for ketones in influencing substrate availability for gluconeogenesis in diabetes.", "contents": "Effect of diabetes mellitus and insulin on the turnover and metabolic response to ketones in man. To determine the effect of diabetes mellitus on ketone removal rates, Na DL-beta-hydroxybutyrate was administered as a continuous three-hour infusion (3 mg./kg./min.) to healthy volunteers and insulin-dependent diabetics in the postabsorptive state. An additional group of healthy controls received intravenous glucose (50 gm.) or glucose and insulin during the ketone infusion to determine the effect of hyperinsulinemia on ketone removal. Following ketone infusion, total blood ketone levels in the diabetics were twofold greater than in controls (p less than 0.001). The metabolic clearance rate of ketones (MCRk) in the diabetics was reduced by 42% from that of controls (p less than 0.001). In contrast, the calculated production rate of ketones (PRk) in diabetics was not consistently different from that observed in controls. In diabetics with normal PRk, MCRk remained significantly below control values (p less than 0.001). The ketone infusion resulted in a fall in plasma glucose and alanine levels in the normals as well as diabetics. However, the decline in plasma glucose induced by the ketone infusion was five- to sixfold greater in the diabetics than in controls (p less than 0.005) and correlated linearly with the decline in plasma alanine (p less than 0.02). Administration of intravenous glucose during an ongoing ketone infusion in normal subjects resulted in 37 +/- 5% reduction in beta-hydroxybutyrate, but no change in acetoacetate concentration. The decline in beta-hydroxybutyrate was two- to threefold greater than would be expected if glucose had acted solely to inhibit endogenous ketone production. Similar results were observed when hyperinsulinemia without hyperglycemia was produced by simultaneous administration of insulin and glucose. It is concluded that (1) ketone disposal is reduced in diabetes even when ketone production is normal, suggesting the rate of ketone utilization may be a more sensitive index of insulin deficiency than is ketone production; (2) hyperinsulinemia stimulates beta-hydroxybutyrate utilization without influencing acetoacetate concentration; and (3) increased blood ketone levels induced by infusion of Na DL-beta-hydroxybutyrate reduce plasma glucose and alanine concentrations in diabetes. These findings thus support a role for insulin in influencing ketone disposal in normal as well as diabetic man and a role for ketones in influencing substrate availability for gluconeogenesis in diabetes.", "PMID": 955305}
{"id": "pubmed23n0032_1592", "title": "Birth order, family size and school failure.", "content": "The effect of birth order on educational outcome in the Netherlands is reported for two major social classes, manual and non-manual. The rates of school failure (those who attended schools for the mentally retarded and who failed lower school) were studied in a population of some 200,000 young adult Dutch males born between 1944 and 1946 and whose families of origin had from one to six children. The data used were the records of the Dutch military pre-induction examination. Rates of school failure rose both with increased birth order and with increased family-size, with the exception of one-child families. School failure rates for the first, middle-and last-born were examined for the two social classes, with family size controlled. In general, school-failure rates were significantly related to birth-order position. For each family size and in both social classes, the last-born were at greater risk of school failure than were the first-born.", "contents": "Birth order, family size and school failure. The effect of birth order on educational outcome in the Netherlands is reported for two major social classes, manual and non-manual. The rates of school failure (those who attended schools for the mentally retarded and who failed lower school) were studied in a population of some 200,000 young adult Dutch males born between 1944 and 1946 and whose families of origin had from one to six children. The data used were the records of the Dutch military pre-induction examination. Rates of school failure rose both with increased birth order and with increased family-size, with the exception of one-child families. School failure rates for the first, middle-and last-born were examined for the two social classes, with family size controlled. In general, school-failure rates were significantly related to birth-order position. For each family size and in both social classes, the last-born were at greater risk of school failure than were the first-born.", "PMID": 955306}
{"id": "pubmed23n0032_1593", "title": "The prevalence of language delay in a population of three-year-old children and its association with general retardation.", "content": "Using a simple assessment of language and a behaviour screening questionnaire, a one-in-four random sample of a total population of three-year-olds was screened for the presence of language delay and behaviour problems. A full psycho-developmental assessment was carried out in children with suspected language delay or a behaviour problem, and in a group of control children, and the sensitivity and specificity of the language screening-test was examined. The results showed the importance of investigating language delay in relation to nonverbal abilities. Of those children with a language age less than two-thirds of their chronological age, 37 per cent also had similarly delayed non-verbal mental abilities. 50 per cent of the children with a language age of less than 30 months were generally retarded in their non-verbal abilities. The estimated prevalence of delayed language development is 31 per 1000, of specific language delay 5-7per 1000, and of severe retardation 4-2 per 1000. Boys were most likely than girls to have language and general developmental delay.", "contents": "The prevalence of language delay in a population of three-year-old children and its association with general retardation. Using a simple assessment of language and a behaviour screening questionnaire, a one-in-four random sample of a total population of three-year-olds was screened for the presence of language delay and behaviour problems. A full psycho-developmental assessment was carried out in children with suspected language delay or a behaviour problem, and in a group of control children, and the sensitivity and specificity of the language screening-test was examined. The results showed the importance of investigating language delay in relation to nonverbal abilities. Of those children with a language age less than two-thirds of their chronological age, 37 per cent also had similarly delayed non-verbal mental abilities. 50 per cent of the children with a language age of less than 30 months were generally retarded in their non-verbal abilities. The estimated prevalence of delayed language development is 31 per 1000, of specific language delay 5-7per 1000, and of severe retardation 4-2 per 1000. Boys were most likely than girls to have language and general developmental delay.", "PMID": 955307}
{"id": "pubmed23n0032_1594", "title": "Tactile-perceptual functioning in relation to intellectual, cognitive and reading skills in younger and older normal children.", "content": "Forty-eight 12- to 14-years-olds and 40 six- to eight-year-old normal boys and girls were distributed into two groups on the basis of the number of errors they made on several tests of tactile-perceptual ability. The groups were then compared on measures of intellectual, cognitive and reading ability. In the older group, children with poor tactile-perceptual skills performed significantly less well on the other measures than children with few tactile-perceptual errors. This relationship was not so clearly demonstrated in the younger groups. The results are discussed in terms of individual differences in brain function.", "contents": "Tactile-perceptual functioning in relation to intellectual, cognitive and reading skills in younger and older normal children. Forty-eight 12- to 14-years-olds and 40 six- to eight-year-old normal boys and girls were distributed into two groups on the basis of the number of errors they made on several tests of tactile-perceptual ability. The groups were then compared on measures of intellectual, cognitive and reading ability. In the older group, children with poor tactile-perceptual skills performed significantly less well on the other measures than children with few tactile-perceptual errors. This relationship was not so clearly demonstrated in the younger groups. The results are discussed in terms of individual differences in brain function.", "PMID": 955308}
{"id": "pubmed23n0032_1595", "title": "Early hospital admissions and later disturbances of behaviour: an attempted replication of Douglas' findings.", "content": "This study confirms the findings of Douglas (1975) that single hospital admissions of children for up to a week carry no increased risk of later emotional or behavioural disturbance. The study also confirms Douglas' finding that repeated hospital admissions are significantly associated with disturbance in later childhood. The association probably applies to both emotional and conduct disorders, is most marked in the case of children from disadvantaged homes, and it may well reflect a causal influence. The validity of the finding is strengthened by the fact that detailed psychiatric assessments gave rise to much the same findings as did teacher questionnaire scores. However, in both cases, repeated hospital admissions are associated with only a small minority of disorers and account for little of the variance in children's behaviour.", "contents": "Early hospital admissions and later disturbances of behaviour: an attempted replication of Douglas' findings. This study confirms the findings of Douglas (1975) that single hospital admissions of children for up to a week carry no increased risk of later emotional or behavioural disturbance. The study also confirms Douglas' finding that repeated hospital admissions are significantly associated with disturbance in later childhood. The association probably applies to both emotional and conduct disorders, is most marked in the case of children from disadvantaged homes, and it may well reflect a causal influence. The validity of the finding is strengthened by the fact that detailed psychiatric assessments gave rise to much the same findings as did teacher questionnaire scores. However, in both cases, repeated hospital admissions are associated with only a small minority of disorers and account for little of the variance in children's behaviour.", "PMID": 955309}
{"id": "pubmed23n0032_1596", "title": "Obstetric history and the heart-rate response of newborns to sound.", "content": "Two groups of clinically normal newborns, differing in the number of non-optimal factors in their obstetric history, were compared by measuring heart-rate response to a series of auditory stimuli. There was a significant difference between the groups in the direction of the average heart-rate response. The high risk\" group showed more heart-rate acceleration and less deceleration compared with the \"low-risk\" group. The heart-rate responses were significantly related to the number of non-optimal obstetric conditions and to parity, but not to the maternal pre-delivery medication score. The greater the \"risk\" at birth, the less was the deceleration; the greater the maternal parity, the less did acceleration occur in response to auditory stimulation. There was no significant difference between the high-risk and low-rsik groups in heart-rate response trends over trials. Both groups showed reliable diminution of deceleration heart-rate response over trials.", "contents": "Obstetric history and the heart-rate response of newborns to sound. Two groups of clinically normal newborns, differing in the number of non-optimal factors in their obstetric history, were compared by measuring heart-rate response to a series of auditory stimuli. There was a significant difference between the groups in the direction of the average heart-rate response. The high risk\" group showed more heart-rate acceleration and less deceleration compared with the \"low-risk\" group. The heart-rate responses were significantly related to the number of non-optimal obstetric conditions and to parity, but not to the maternal pre-delivery medication score. The greater the \"risk\" at birth, the less was the deceleration; the greater the maternal parity, the less did acceleration occur in response to auditory stimulation. There was no significant difference between the high-risk and low-rsik groups in heart-rate response trends over trials. Both groups showed reliable diminution of deceleration heart-rate response over trials.", "PMID": 955310}
{"id": "pubmed23n0032_1597", "title": "Prognosis of infants of diabetic mothers in relation to neonatal hypoglycaemia.", "content": "A prospective follow-up study was conducted to determine whether neonatal hypoglycaemia in infants of diabetic mothers affects subsequent neurological and intellectual performance. 37 such infants (25 hypoglycaemic and 12 non-hypoglycaemic) were examined for physical, neurological and developmental performance at an average age of 4 1/2 years. 11 children were abnormal, with generalised retardation and neurological abnormalities, or delays in particular areas of development; three children were possibly abnormal; and 23 children were normal. Abnormality at follow-up could not be related to neonatal blood glucose level, to the duration of hypoglycaemia or to any other measurement made in the neonatal period, nor to any factor relating to the maternal diabetes. Compared with the normal children, the abnormal group had slightly small head-circumferences at birth relative to their gestational age, but a follow-up there was no difference in head size. At follow-up the children of diabetic mothers tended to be shorter than average. The poor prognosis of the infants in this study was not due to brain damage caused by neonatal hypoglycaemia.", "contents": "Prognosis of infants of diabetic mothers in relation to neonatal hypoglycaemia. A prospective follow-up study was conducted to determine whether neonatal hypoglycaemia in infants of diabetic mothers affects subsequent neurological and intellectual performance. 37 such infants (25 hypoglycaemic and 12 non-hypoglycaemic) were examined for physical, neurological and developmental performance at an average age of 4 1/2 years. 11 children were abnormal, with generalised retardation and neurological abnormalities, or delays in particular areas of development; three children were possibly abnormal; and 23 children were normal. Abnormality at follow-up could not be related to neonatal blood glucose level, to the duration of hypoglycaemia or to any other measurement made in the neonatal period, nor to any factor relating to the maternal diabetes. Compared with the normal children, the abnormal group had slightly small head-circumferences at birth relative to their gestational age, but a follow-up there was no difference in head size. At follow-up the children of diabetic mothers tended to be shorter than average. The poor prognosis of the infants in this study was not due to brain damage caused by neonatal hypoglycaemia.", "PMID": 955311}
{"id": "pubmed23n0032_1598", "title": "Central nervous system damage as a possible component of unexpected deaths in infancy.", "content": "A longitudinal study of 1553 infants, designed to provide early indicators of gross developmental problems by using a behavioral assessment scale, included 12 infants who later died suddenly and unexpectedly. In a retrospective examination of neonatal records, considerable evidence of central nervous system damage was found. Anoxic conditions and even possible seizure activity were implicated. Abnormalities of muscle tonus, skin color and cry were found, together with some visual problems. These findings on behavioral assessment in the neonatal period appear to identify a population which is at greater risk of sudden death in infancy. The need for oxygen therapy as a high-risk indicator of sudden unexpected death has been reported in a previous study and is further substantiated by the present findings: they are also compatible with thos of Naeye (1973), who found long-term hypoxic conditions in autopsy studies on the sudden infant death syndrome. It is suggested that the possibility of central nervous system involvement in the aetiology of the sudden infant death syndrome should be more thoroughly investigated.", "contents": "Central nervous system damage as a possible component of unexpected deaths in infancy. A longitudinal study of 1553 infants, designed to provide early indicators of gross developmental problems by using a behavioral assessment scale, included 12 infants who later died suddenly and unexpectedly. In a retrospective examination of neonatal records, considerable evidence of central nervous system damage was found. Anoxic conditions and even possible seizure activity were implicated. Abnormalities of muscle tonus, skin color and cry were found, together with some visual problems. These findings on behavioral assessment in the neonatal period appear to identify a population which is at greater risk of sudden death in infancy. The need for oxygen therapy as a high-risk indicator of sudden unexpected death has been reported in a previous study and is further substantiated by the present findings: they are also compatible with thos of Naeye (1973), who found long-term hypoxic conditions in autopsy studies on the sudden infant death syndrome. It is suggested that the possibility of central nervous system involvement in the aetiology of the sudden infant death syndrome should be more thoroughly investigated.", "PMID": 955312}
{"id": "pubmed23n0032_1599", "title": "Immunological status in tuberous sclerosis.", "content": "Cellular and humoral immunity, and lymphoid organ pathology, have been investigated in 10 institutionalized patients with tuberous sclerosis and 10 institutionalized matched controls without the disease. Type and incidence of infections and tumours were reviewed for each group, as was current medication. Elevated serum IgM levels were found in the patients with tuberous sclerosis, but no immunological deficiency of either cellular of humoral immunity was found, nor was there a difference in infection between the groups. Only patients with tuberous sclerosis had evidence of neoplasia. No morphological or histological abnormalities of lymph nodes, spleen or thymus were present. Explanations for the difference between tuberous sclerosis and ataxia telangiectasia are discussed, together with the effect of immuno-surveillance on the development of malignancy.", "contents": "Immunological status in tuberous sclerosis. Cellular and humoral immunity, and lymphoid organ pathology, have been investigated in 10 institutionalized patients with tuberous sclerosis and 10 institutionalized matched controls without the disease. Type and incidence of infections and tumours were reviewed for each group, as was current medication. Elevated serum IgM levels were found in the patients with tuberous sclerosis, but no immunological deficiency of either cellular of humoral immunity was found, nor was there a difference in infection between the groups. Only patients with tuberous sclerosis had evidence of neoplasia. No morphological or histological abnormalities of lymph nodes, spleen or thymus were present. Explanations for the difference between tuberous sclerosis and ataxia telangiectasia are discussed, together with the effect of immuno-surveillance on the development of malignancy.", "PMID": 955313}
{"id": "pubmed23n0032_1600", "title": "Hydrocephalus and congenital clasped thumbs: a case report with electromyographic evaluation.", "content": "A one-month-old male infant with hydrocephalus, aqueductal stenosis and congenital clasped thumbs is reported. Electromyographic evaluation supported the hypothesis that the clasped thumbs were a consequence of flexion-extension imbalance, with subsequent muscle contracture secondary to hypoplasia or aplasia of the thumb extensors.", "contents": "Hydrocephalus and congenital clasped thumbs: a case report with electromyographic evaluation. A one-month-old male infant with hydrocephalus, aqueductal stenosis and congenital clasped thumbs is reported. Electromyographic evaluation supported the hypothesis that the clasped thumbs were a consequence of flexion-extension imbalance, with subsequent muscle contracture secondary to hypoplasia or aplasia of the thumb extensors.", "PMID": 955314}
{"id": "pubmed23n0032_1601", "title": "A comparison of the acid and gastrin secretory responses to hypoglycaemia and meals in duodenal ulcer with and without acid hypersecretion to pentagastrin.", "content": "In 60 uncomplicated duodenal ulcers (DU), maximum acid output (MAO) and acid output following insulin hypoglycaemia (IAO) were measured, and in 30 patients also the serum gastrin responses to insulin and to standard meals, In 7 normal controls, all 4 items were measured. Thirty-six of the DU had MAO within normal limits (normosecretors) and 24 were hypersecretors. Hypoglycaemic vagal response was higher in all DU than in normals and was, in normo-secreting DU associated with an abnormally large functioning G cell mass. This mass is not increased in hypersecreting DU and the greater vagal capacity is related to a supranormal parietal cell mass. An unknown mechanism, perhaps genetic, directs the trophic effects of an increased vagal drive to the gastrin cell mass in one group of DU and to the parietal cell mass in the other.", "contents": "A comparison of the acid and gastrin secretory responses to hypoglycaemia and meals in duodenal ulcer with and without acid hypersecretion to pentagastrin. In 60 uncomplicated duodenal ulcers (DU), maximum acid output (MAO) and acid output following insulin hypoglycaemia (IAO) were measured, and in 30 patients also the serum gastrin responses to insulin and to standard meals, In 7 normal controls, all 4 items were measured. Thirty-six of the DU had MAO within normal limits (normosecretors) and 24 were hypersecretors. Hypoglycaemic vagal response was higher in all DU than in normals and was, in normo-secreting DU associated with an abnormally large functioning G cell mass. This mass is not increased in hypersecreting DU and the greater vagal capacity is related to a supranormal parietal cell mass. An unknown mechanism, perhaps genetic, directs the trophic effects of an increased vagal drive to the gastrin cell mass in one group of DU and to the parietal cell mass in the other.", "PMID": 955321}
{"id": "pubmed23n0032_1602", "title": "Serum and intestinal fluid immunoglobulins and jejunal IgA secretion in Crohn's disease.", "content": "Immunoglobulins in serum and proximal intestinal fluids and secretion of IgA by cultured jejunal mucosa were measured in 12 healthy subjects and 36 patients with Crohn's disease. Concentrations of IgA, IgG, IgM, and IgE in serum and intestinal fluids were similar in the two groups, except for increased serum IgA concentrations in the patients. Elevation of IgA and chronicity of disease were correlated, which suggests that the IgA alteration was a response to duration of disease rather than a primary pathogenetic factor. IgA secretion by cultured jejunum was similar in control and patient groups. Thus, no evidence was found that abnormalities of secretory immunoglobulins are pathogenetically involved in Crohn's disease.", "contents": "Serum and intestinal fluid immunoglobulins and jejunal IgA secretion in Crohn's disease. Immunoglobulins in serum and proximal intestinal fluids and secretion of IgA by cultured jejunal mucosa were measured in 12 healthy subjects and 36 patients with Crohn's disease. Concentrations of IgA, IgG, IgM, and IgE in serum and intestinal fluids were similar in the two groups, except for increased serum IgA concentrations in the patients. Elevation of IgA and chronicity of disease were correlated, which suggests that the IgA alteration was a response to duration of disease rather than a primary pathogenetic factor. IgA secretion by cultured jejunum was similar in control and patient groups. Thus, no evidence was found that abnormalities of secretory immunoglobulins are pathogenetically involved in Crohn's disease.", "PMID": 955322}
{"id": "pubmed23n0032_1603", "title": "LDH isoenzyme pattern of uninvolved gastric mucosa of patients with gastric carcinoma and benign gastric disease.", "content": "The LDH isoenzyme pattern of the uninvolved mucosa of gastric cancer patients differs as regards the LDH isoenzyme pattern from that of similar tissue of patients with benign gastric disease; the former tissue is characterised by a high M/H ratio of the LDH isoenzymes (M and H sub-units). A high M/H ratio characterises antral mucosa when the latter is compared with fundic mucosa. Mucosa showing superficial and atrophic gastritis also has a higher M/H ratio, whereas the presence of intestinal metaplasia does not appear to influence the M/H ratio. These observations are consistent with the concept that the tissue from which the cancer arose may possess a pre-malignant biochemical lesion.", "contents": "LDH isoenzyme pattern of uninvolved gastric mucosa of patients with gastric carcinoma and benign gastric disease. The LDH isoenzyme pattern of the uninvolved mucosa of gastric cancer patients differs as regards the LDH isoenzyme pattern from that of similar tissue of patients with benign gastric disease; the former tissue is characterised by a high M/H ratio of the LDH isoenzymes (M and H sub-units). A high M/H ratio characterises antral mucosa when the latter is compared with fundic mucosa. Mucosa showing superficial and atrophic gastritis also has a higher M/H ratio, whereas the presence of intestinal metaplasia does not appear to influence the M/H ratio. These observations are consistent with the concept that the tissue from which the cancer arose may possess a pre-malignant biochemical lesion.", "PMID": 955323}
{"id": "pubmed23n0032_1604", "title": "The distribution and enteric loss of 51Cr-labelled lymphocytes in normal subjects and in patients with coeliac disease and other disorders of the small intestine.", "content": "Peripherally harvested lymphocytes have been labelled with 51Cr, reinjected into human subjects and their distribution then studied. Evidence is presented which suggests faecal loss of 51Cr represents loss of T lymphocytes and that there is normally a pathway of lymphocyte removal into the gut of probable importance in lymphocyte migration streams. In 9 normal subjects, without structural intestinal disease, faecal loss of lymphocytes over 5 days was 0.20% (SEM +/- 0.06) whereas in 5 patients with untreated coeliac disease faecal loss was 1.13 +/- 0.34%, in 7 with Crohn's disease it was 1.01 +/- 0.21% and in 5 with intestinal lymphangiectasia loss was 0.61 +/- 0.10%. In 1 patient with acute tropical sprue, enteric loss was 0.97%. By contrast, faecal loss was normal in 3 coeliac patients in remission on a gluten-free diet. Measurements were also made using an external counter. In contrast to the normals, where count rates steadily diminished, an increasing activity was recorded over the umbilicus over 7 days after dose administration in all the disease categories studied with the exception of the treated coeliacs. The finding of an increased enteric loss of lymphocytes may explain many of the immunological abnormalities in the conditions studied.", "contents": "The distribution and enteric loss of 51Cr-labelled lymphocytes in normal subjects and in patients with coeliac disease and other disorders of the small intestine. Peripherally harvested lymphocytes have been labelled with 51Cr, reinjected into human subjects and their distribution then studied. Evidence is presented which suggests faecal loss of 51Cr represents loss of T lymphocytes and that there is normally a pathway of lymphocyte removal into the gut of probable importance in lymphocyte migration streams. In 9 normal subjects, without structural intestinal disease, faecal loss of lymphocytes over 5 days was 0.20% (SEM +/- 0.06) whereas in 5 patients with untreated coeliac disease faecal loss was 1.13 +/- 0.34%, in 7 with Crohn's disease it was 1.01 +/- 0.21% and in 5 with intestinal lymphangiectasia loss was 0.61 +/- 0.10%. In 1 patient with acute tropical sprue, enteric loss was 0.97%. By contrast, faecal loss was normal in 3 coeliac patients in remission on a gluten-free diet. Measurements were also made using an external counter. In contrast to the normals, where count rates steadily diminished, an increasing activity was recorded over the umbilicus over 7 days after dose administration in all the disease categories studied with the exception of the treated coeliacs. The finding of an increased enteric loss of lymphocytes may explain many of the immunological abnormalities in the conditions studied.", "PMID": 955324}
{"id": "pubmed23n0032_1605", "title": "Effect of secretin, glucagon and VIP on gallbladder contraction.", "content": "The effects on gallbladder contraction of three structurally related peptides, secretin, glucagon and the vasoactive intestinal peptide (VIP) have been compared in urethane-anesthetized guinea pigs. Secretin and glucagon had no effect alone but augmented cholecystokinin (CCK)-induced contractions. VIP decreased CCK-induced contractions.", "contents": "Effect of secretin, glucagon and VIP on gallbladder contraction. The effects on gallbladder contraction of three structurally related peptides, secretin, glucagon and the vasoactive intestinal peptide (VIP) have been compared in urethane-anesthetized guinea pigs. Secretin and glucagon had no effect alone but augmented cholecystokinin (CCK)-induced contractions. VIP decreased CCK-induced contractions.", "PMID": 955325}
{"id": "pubmed23n0032_1606", "title": "Effects of acetylsalicylic acid (ASA), ASA plus L-glutamine and L-glutamine on healing of chronic gastric ulcer in the rat.", "content": "A chronic gastric ulcer model was produced in rats by the subserosal injection of 20% acetic acid solution (0.015 ml) in order to examine whether (1) acetylsalicylic acid (ASA) irritates the chronic gastric ulcer in active or healed or diminished stage, (2) L-glutamine, given together with ASA, inhibits the adverse effect of ASA. Oral ASA 200 mg/kg/day, given in two divided doses for 10 consecutive days, apparently delayed the healing of the gastric ulcer and irritated the healed ulcer to reulcerate. L-Glutamine, 1,500 mg/kg/day, which was given together with ASA in two divided doses, markedly protected the gastric ulcer both in active and healed stages from the deleterious activity of ASA.", "contents": "Effects of acetylsalicylic acid (ASA), ASA plus L-glutamine and L-glutamine on healing of chronic gastric ulcer in the rat. A chronic gastric ulcer model was produced in rats by the subserosal injection of 20% acetic acid solution (0.015 ml) in order to examine whether (1) acetylsalicylic acid (ASA) irritates the chronic gastric ulcer in active or healed or diminished stage, (2) L-glutamine, given together with ASA, inhibits the adverse effect of ASA. Oral ASA 200 mg/kg/day, given in two divided doses for 10 consecutive days, apparently delayed the healing of the gastric ulcer and irritated the healed ulcer to reulcerate. L-Glutamine, 1,500 mg/kg/day, which was given together with ASA in two divided doses, markedly protected the gastric ulcer both in active and healed stages from the deleterious activity of ASA.", "PMID": 955326}
{"id": "pubmed23n0032_1607", "title": "Double wave cohort increase for oesophageal and laryngeal cancer in France in relation to reduced alcohol consumption during the second world war.", "content": "Mortality from oesophageal cancer is increasing in France. A cohort analysis indicates that there were two successive waves of increase separated by a non-increase interval for the cohorts born between 1902 and 1916. A similar effect was observed for laryngeal cancer and liver cirrhosis but not for lung and pancreas cancer. This might be related to the reduction of alcohol consumption during the second world war.", "contents": "Double wave cohort increase for oesophageal and laryngeal cancer in France in relation to reduced alcohol consumption during the second world war. Mortality from oesophageal cancer is increasing in France. A cohort analysis indicates that there were two successive waves of increase separated by a non-increase interval for the cohorts born between 1902 and 1916. A similar effect was observed for laryngeal cancer and liver cirrhosis but not for lung and pancreas cancer. This might be related to the reduction of alcohol consumption during the second world war.", "PMID": 955327}
{"id": "pubmed23n0032_1608", "title": "The medical treatment of cholesterol gallstones: experience with chenodeoxycholic acid.", "content": "Chenodeoxycholic acid has been administered to 50 patients with cholelithiasis and 8 with choledocholithiasis for a period of 15 months at doses varying between 2- and 15 mg/kg/day. Among the 50 patients with cholelithiasis, complete dissolution occurred in 16 and partial dissolution in 15 cases. Among the 8 patients with choledocholithiasis, complete dissolution occurred in 3 and partial dissolution in 2 cases. Lower doses were adopted in an attempt to determine the minimum effective daily dose required to produce gallstone dissolution and to lower the cholesterol saturation index (mean 1.52 +/- 0.73 before and 0.98 +/- 0.36 after treatment). Routine liver function tests were carried out before, during and after treatment; no significant alterations were produced by therapy. Studies of the antipyrine half-life, to estimate hepatic microsomial function were found to be normal (mean 13.61 +/- 2.62 before and 13.21 +/- 3.04 after treatment). In addition, histological investigations of the liver were described. This study confirms the efficacy of chenodeoxycholic acid in the treatment of cholesterol gallstones and gives an indication of the minimum effective dose (7.8 mg/kg/day). At the present doses, it has been shown that there are no substantial modifications in fundamental liver function.", "contents": "The medical treatment of cholesterol gallstones: experience with chenodeoxycholic acid. Chenodeoxycholic acid has been administered to 50 patients with cholelithiasis and 8 with choledocholithiasis for a period of 15 months at doses varying between 2- and 15 mg/kg/day. Among the 50 patients with cholelithiasis, complete dissolution occurred in 16 and partial dissolution in 15 cases. Among the 8 patients with choledocholithiasis, complete dissolution occurred in 3 and partial dissolution in 2 cases. Lower doses were adopted in an attempt to determine the minimum effective daily dose required to produce gallstone dissolution and to lower the cholesterol saturation index (mean 1.52 +/- 0.73 before and 0.98 +/- 0.36 after treatment). Routine liver function tests were carried out before, during and after treatment; no significant alterations were produced by therapy. Studies of the antipyrine half-life, to estimate hepatic microsomial function were found to be normal (mean 13.61 +/- 2.62 before and 13.21 +/- 3.04 after treatment). In addition, histological investigations of the liver were described. This study confirms the efficacy of chenodeoxycholic acid in the treatment of cholesterol gallstones and gives an indication of the minimum effective dose (7.8 mg/kg/day). At the present doses, it has been shown that there are no substantial modifications in fundamental liver function.", "PMID": 955328}
{"id": "pubmed23n0032_1609", "title": "The effect of intravenous theophylline ethylenediamine on serum gastrin concentration in control subjects and patients with duodenal ulcers and Zollinger-Ellison syndrome.", "content": "Serum gastrin concentrations were determined after an intravenous infusion of theophylline ethylenediamine (aminophylline) in 10 patients with duodenal ulcer, 9 patients with Zollinger-Ellison syndrome, 7 control subjects and 3 patients with pernicious anemia. Increases of serum gastrin levels were observed in each group. It is concluded that an aminophylline test would not be useful in the differential diagnosis of the Zollinger-Ellison syndrome.", "contents": "The effect of intravenous theophylline ethylenediamine on serum gastrin concentration in control subjects and patients with duodenal ulcers and Zollinger-Ellison syndrome. Serum gastrin concentrations were determined after an intravenous infusion of theophylline ethylenediamine (aminophylline) in 10 patients with duodenal ulcer, 9 patients with Zollinger-Ellison syndrome, 7 control subjects and 3 patients with pernicious anemia. Increases of serum gastrin levels were observed in each group. It is concluded that an aminophylline test would not be useful in the differential diagnosis of the Zollinger-Ellison syndrome.", "PMID": 955329}
{"id": "pubmed23n0032_1610", "title": "A demonstration that the ratios between human pancreatic enzymes can change in 4 days.", "content": "Electrophoretic studies of inactive ductal pancreatic secretions, collected on five successive days following operation, demonstrated up to 18 protein bands. Comparison of the densitometric scans on the first and fourth days was as follows: the patterns from different individuals appeared similar during the periods of slow secretion immediately following surgery, there were obvious changes in the proportions of the slower-moving enzymes between the first and fourth days; the most apparent alteration was in the trypsinogen 2 band which was markedly higher on the fourth as opposed to the first day, and during the 4th-6th days, the patterns for a given individual resembled one another, showing only minor changes among the 8-hour collections.", "contents": "A demonstration that the ratios between human pancreatic enzymes can change in 4 days. Electrophoretic studies of inactive ductal pancreatic secretions, collected on five successive days following operation, demonstrated up to 18 protein bands. Comparison of the densitometric scans on the first and fourth days was as follows: the patterns from different individuals appeared similar during the periods of slow secretion immediately following surgery, there were obvious changes in the proportions of the slower-moving enzymes between the first and fourth days; the most apparent alteration was in the trypsinogen 2 band which was markedly higher on the fourth as opposed to the first day, and during the 4th-6th days, the patterns for a given individual resembled one another, showing only minor changes among the 8-hour collections.", "PMID": 955330}
{"id": "pubmed23n0032_1611", "title": "Effect of heparin on lecithin: cholesterol acyltransferase and lipids in cholestasis.", "content": "The effect of heparin on the proportion of cholesterol esters, lecithin/lysolecithin ratio, and lecithin: cholesterol acyltransferase (LCAT) activity was studied in twelve patients with cholestasis. A good correlation was observed between a decreased LCAT activity and the proportion of cholesterol esters, and no consistent variation was noted after heparin. The lecithin/lysolecithin ratio was increased in 7 patients and became normal after heparin in 6 cases. The clearance of lipoprotein-X after heparin seems to be not related to LCAT activity. These results demonstrate that heparin does not modify the LCAT activity, and suggest that heparin induces an increase of phospholipase activity in some patients with cholestasis.", "contents": "Effect of heparin on lecithin: cholesterol acyltransferase and lipids in cholestasis. The effect of heparin on the proportion of cholesterol esters, lecithin/lysolecithin ratio, and lecithin: cholesterol acyltransferase (LCAT) activity was studied in twelve patients with cholestasis. A good correlation was observed between a decreased LCAT activity and the proportion of cholesterol esters, and no consistent variation was noted after heparin. The lecithin/lysolecithin ratio was increased in 7 patients and became normal after heparin in 6 cases. The clearance of lipoprotein-X after heparin seems to be not related to LCAT activity. These results demonstrate that heparin does not modify the LCAT activity, and suggest that heparin induces an increase of phospholipase activity in some patients with cholestasis.", "PMID": 955331}
{"id": "pubmed23n0032_1612", "title": "The effects of alterations in villus shape on the intestinal mucosal surface of the albino rat; the relationship between mucosal surface area and the crypts.", "content": "The villi and crypts of the small intestine of the albino rat (Rattus norvigicus) have been enumerated and measured at different ages from 2 weeks to 8 months. The number of villi increased up to the third month of age and the number of crypts up to the eighth month.  In the proximal intestine, the mean length of the villus base increased  up to the fifth month as ridge-shaped villi were formed. Villus height was greater proximally than distally and this, and the crypt depth, remained constant from the end of the first month of age. The total villus circumference per unit area of intestine, and the villus circumference per crypt was the same proximally and distally, and was relatively constant after the first month of age. The total circumference of the crypt mouths per square millimeter of intestine was the same proximally and distally and, at all ages, was greater than the total villus circumference. The villus surface area per square millimeter of intestine, or per crypt, remained relatively constant after the first month and was greater proximally than distally, due only to the taller villi proximally. The change from finger-shaped to ridge-shaped villi did not affect the villus mucosal surface area. The changes in villus shape are probably not determined by differences in the rate of crypt cell production.", "contents": "The effects of alterations in villus shape on the intestinal mucosal surface of the albino rat; the relationship between mucosal surface area and the crypts. The villi and crypts of the small intestine of the albino rat (Rattus norvigicus) have been enumerated and measured at different ages from 2 weeks to 8 months. The number of villi increased up to the third month of age and the number of crypts up to the eighth month.  In the proximal intestine, the mean length of the villus base increased  up to the fifth month as ridge-shaped villi were formed. Villus height was greater proximally than distally and this, and the crypt depth, remained constant from the end of the first month of age. The total villus circumference per unit area of intestine, and the villus circumference per crypt was the same proximally and distally, and was relatively constant after the first month of age. The total circumference of the crypt mouths per square millimeter of intestine was the same proximally and distally and, at all ages, was greater than the total villus circumference. The villus surface area per square millimeter of intestine, or per crypt, remained relatively constant after the first month and was greater proximally than distally, due only to the taller villi proximally. The change from finger-shaped to ridge-shaped villi did not affect the villus mucosal surface area. The changes in villus shape are probably not determined by differences in the rate of crypt cell production.", "PMID": 955332}
{"id": "pubmed23n0032_1613", "title": "Combined lactose-D-xylose tolerance test in infancy.", "content": "A modified combined oral lactose-D-xylose tolerance test done on ten normal infants showed peak levels of blood glucose and D-xylose levels comparable to the peaks obtained when these tests were done separately in the same subjects. The combined test is recommended to evaluate digestive absorptive function in preference to two separate tolerance tests.", "contents": "Combined lactose-D-xylose tolerance test in infancy. A modified combined oral lactose-D-xylose tolerance test done on ten normal infants showed peak levels of blood glucose and D-xylose levels comparable to the peaks obtained when these tests were done separately in the same subjects. The combined test is recommended to evaluate digestive absorptive function in preference to two separate tolerance tests.", "PMID": 955333}
{"id": "pubmed23n0032_1614", "title": "The effect of vasopressin and triglycyl lysine vasopressin (glypressin) on the splanchnic circulation in cirrhotic patients with portal hypertension.", "content": "In four cirrhotic patients with portal hypertension we have measured some hemodynamic parameters over 60-120 min; portal venous pressure, hepatic venous pressure, mean arterial blood pressure, hepatic blood flow, transsinusoidal vascular resistance and splanchnic oxygen uptake under treatment with Vasopressin (1 IU/min for 10 min) and glypressin (50 mug/kg body weight). The effect on the parameters was less pronounced with glypressin but of longer duration.", "contents": "The effect of vasopressin and triglycyl lysine vasopressin (glypressin) on the splanchnic circulation in cirrhotic patients with portal hypertension. In four cirrhotic patients with portal hypertension we have measured some hemodynamic parameters over 60-120 min; portal venous pressure, hepatic venous pressure, mean arterial blood pressure, hepatic blood flow, transsinusoidal vascular resistance and splanchnic oxygen uptake under treatment with Vasopressin (1 IU/min for 10 min) and glypressin (50 mug/kg body weight). The effect on the parameters was less pronounced with glypressin but of longer duration.", "PMID": 955334}
{"id": "pubmed23n0032_1615", "title": "A reassessment of vascular factors in relation to intravascular coagulation.", "content": "Sural nerve biopsy was performed in twenty-four diabetic patients, with clinical and electrophysiological evidence of diabetic neuropathy. Material from an autopsy case was also examined. Vessels plugged with fibrin were seen within nerve in nine cases. In three cases fibrin was observed tracking into the vessel wall and in four, older thrombus was observed in vessels. Areas of necrosis in nerve bundles were seen in two of the latter. In two patients there had been a preceding episode of intravascular coagulation. Fibrin deposition within small vessels could well play a part in damaging the diabetic nerve and a disturbance of the balance between deposition and removal by fibrinolysis could explain phasic variation in the symptoms of neuropathy.", "contents": "A reassessment of vascular factors in relation to intravascular coagulation. Sural nerve biopsy was performed in twenty-four diabetic patients, with clinical and electrophysiological evidence of diabetic neuropathy. Material from an autopsy case was also examined. Vessels plugged with fibrin were seen within nerve in nine cases. In three cases fibrin was observed tracking into the vessel wall and in four, older thrombus was observed in vessels. Areas of necrosis in nerve bundles were seen in two of the latter. In two patients there had been a preceding episode of intravascular coagulation. Fibrin deposition within small vessels could well play a part in damaging the diabetic nerve and a disturbance of the balance between deposition and removal by fibrinolysis could explain phasic variation in the symptoms of neuropathy.", "PMID": 955335}
{"id": "pubmed23n0032_1616", "title": "Bladder dysfunction and neuropathy in diabetes.", "content": "Established urodynamic and electrophysiological techniques have been applied to assess the frequency and extent of autonomic and peripheral neuropathy in 60 subjects with diabetes mellitus; 38 were diabetics with suggestive symptoms and the others were representative newly diagnosed (11) or treated (11) diabetics. Objective evidence neuropathic bladder dysfunction was detected in 43 of them (71.7%). The commonest abnormality was a hypotonic, insensitive large capacity bladder, which condition was usually asymptomatic. Less freuqently (15%) was this complicated by bladder decompensation and sphincter involvement, resulting in excessive residual urine and infection; some of these had bladder paralysis with chronic painless retention of urine (7%). Electrophysiological studies found a sensory defect in the lower limbs in all tested patients (100%), and in 41 patients (69%) as associated motor conduction abnormality, which was more frequent and marked in the lower than the upper limb. These functional abnormalities appeared to be related to the severity of diabetes, but less to its duration. Indeed of 11 newly diagnosed diabetics tested 7 had a peripheral neuropathy and 4 urodynamic abnormalities. The high incidence of bladder dysfunction and peripheral neuropathy in this series indicates the frequency of subclinical diabetic neuropathy and a factor needing more emphasis in diabetic uropathy.", "contents": "Bladder dysfunction and neuropathy in diabetes. Established urodynamic and electrophysiological techniques have been applied to assess the frequency and extent of autonomic and peripheral neuropathy in 60 subjects with diabetes mellitus; 38 were diabetics with suggestive symptoms and the others were representative newly diagnosed (11) or treated (11) diabetics. Objective evidence neuropathic bladder dysfunction was detected in 43 of them (71.7%). The commonest abnormality was a hypotonic, insensitive large capacity bladder, which condition was usually asymptomatic. Less freuqently (15%) was this complicated by bladder decompensation and sphincter involvement, resulting in excessive residual urine and infection; some of these had bladder paralysis with chronic painless retention of urine (7%). Electrophysiological studies found a sensory defect in the lower limbs in all tested patients (100%), and in 41 patients (69%) as associated motor conduction abnormality, which was more frequent and marked in the lower than the upper limb. These functional abnormalities appeared to be related to the severity of diabetes, but less to its duration. Indeed of 11 newly diagnosed diabetics tested 7 had a peripheral neuropathy and 4 urodynamic abnormalities. The high incidence of bladder dysfunction and peripheral neuropathy in this series indicates the frequency of subclinical diabetic neuropathy and a factor needing more emphasis in diabetic uropathy.", "PMID": 955336}
{"id": "pubmed23n0032_1617", "title": "Humoral immunodeficiency to bacterial antigens in patients with juvenile onset diabetes mellitus.", "content": "Humoral immunity to bacterial antigens was tested in 49 tissue typed patients with juvenile onset diabetes mellitus (JOD) and in 50 healthy controls. The number of patients with agglutinins to E. coli and staphylococci was significantly lower compared to controls (p less than 0.001, p less than 0.01 respectively). Missing antibody formation to pertussis and diphtheria toxoid could also be detected in a higher percentage of JOD patients than of controls (p less than 0.05; p congruent to 0.05, respectively). By contrast heteroagglutinins to sheep and rabbit erythrocytes were found in similar proportion in both groups and the values of immunoglobulin serum concentrations showed no difference between patients and controls. In addition no correlation between antibody formation and genes of the HLA complex was found. It is suggested that the severely reduced agglutinin formation to bacteria antigens might be partly responsible for susceptibility to bacterial infections in juvenile diabetics.", "contents": "Humoral immunodeficiency to bacterial antigens in patients with juvenile onset diabetes mellitus. Humoral immunity to bacterial antigens was tested in 49 tissue typed patients with juvenile onset diabetes mellitus (JOD) and in 50 healthy controls. The number of patients with agglutinins to E. coli and staphylococci was significantly lower compared to controls (p less than 0.001, p less than 0.01 respectively). Missing antibody formation to pertussis and diphtheria toxoid could also be detected in a higher percentage of JOD patients than of controls (p less than 0.05; p congruent to 0.05, respectively). By contrast heteroagglutinins to sheep and rabbit erythrocytes were found in similar proportion in both groups and the values of immunoglobulin serum concentrations showed no difference between patients and controls. In addition no correlation between antibody formation and genes of the HLA complex was found. It is suggested that the severely reduced agglutinin formation to bacteria antigens might be partly responsible for susceptibility to bacterial infections in juvenile diabetics.", "PMID": 955337}
{"id": "pubmed23n0032_1618", "title": "Gastric inhibitory polypeptide (GIP), gastrin and insulin: response to test meal in coeliac disease and after duodeno-pancreatectomy.", "content": "The response of serum immunoreactive gastric inhibitory polypeptide (IR-GIP), gastrin (IRG) and insulin (IRI) to a mixed standard meal was measured in 15 controls, 6 patients with coeliac disease, 26 patients with chronic pancreatitis and partial duodenopancreatectomy (Whipple's procedure). Serum levels of IR-GIP, IRG and IRI were significantly reduced in patients with coeliac disease. The serum glucose increase was significantly smaller only during the first hour after the meal. Since small intestinal GIP- and G-cells are situated mainly in the glands of duodenal and jejunal mucosa their absolute number is not significantly reduced in coeliac disease. It is suggested that the release of IR-GIP and duodenal IRG is influenced by the rate of absorption of nutrients. In patients with chronic pancreatitis the IR-GIP release is significantly greater than in controls, the IRG release normal and the IRI response delayed. After Whipple's procedure the IR-GIP response is increased significantly while the IRG secretion is abolished. This demonstrates that the duodenum is not necessary for GIP release and that pancreatic and jejunal gastrin are without clinical significance.", "contents": "Gastric inhibitory polypeptide (GIP), gastrin and insulin: response to test meal in coeliac disease and after duodeno-pancreatectomy. The response of serum immunoreactive gastric inhibitory polypeptide (IR-GIP), gastrin (IRG) and insulin (IRI) to a mixed standard meal was measured in 15 controls, 6 patients with coeliac disease, 26 patients with chronic pancreatitis and partial duodenopancreatectomy (Whipple's procedure). Serum levels of IR-GIP, IRG and IRI were significantly reduced in patients with coeliac disease. The serum glucose increase was significantly smaller only during the first hour after the meal. Since small intestinal GIP- and G-cells are situated mainly in the glands of duodenal and jejunal mucosa their absolute number is not significantly reduced in coeliac disease. It is suggested that the release of IR-GIP and duodenal IRG is influenced by the rate of absorption of nutrients. In patients with chronic pancreatitis the IR-GIP release is significantly greater than in controls, the IRG release normal and the IRI response delayed. After Whipple's procedure the IR-GIP response is increased significantly while the IRG secretion is abolished. This demonstrates that the duodenum is not necessary for GIP release and that pancreatic and jejunal gastrin are without clinical significance.", "PMID": 955338}
{"id": "pubmed23n0032_1619", "title": "Lower esophageal sphincter and gastric acid response to intravenous infusions of synthetic human gastrin I heptadecapeptide.", "content": "Human gastrin I heptadecapeptide was administered by constant intravenous infusion to 7 adult male volunteers without gastrointestinal disease, and serum gastrin concentrations and responses of the lower esophageal sphincter (LES) pressure and gastric acid output were measured and compared. Significant responses in LES pressure were found at gastrin infusion rates which produced submaximal gastric acid output. These LES pressure responses were quantitatively smaller than those occurring concomitantly with similar total immunoreactive gastrin levels evoked by administration of a protein meal in the same subjects. These observations suggest that, although increases in serum gastrin concentrations may contribute to increases in LES pressure seen under physiological conditions after feeding, the increase in LES pressure produced by feeding cannot be explained exclusively on the basis of endogenous gastrin release.", "contents": "Lower esophageal sphincter and gastric acid response to intravenous infusions of synthetic human gastrin I heptadecapeptide. Human gastrin I heptadecapeptide was administered by constant intravenous infusion to 7 adult male volunteers without gastrointestinal disease, and serum gastrin concentrations and responses of the lower esophageal sphincter (LES) pressure and gastric acid output were measured and compared. Significant responses in LES pressure were found at gastrin infusion rates which produced submaximal gastric acid output. These LES pressure responses were quantitatively smaller than those occurring concomitantly with similar total immunoreactive gastrin levels evoked by administration of a protein meal in the same subjects. These observations suggest that, although increases in serum gastrin concentrations may contribute to increases in LES pressure seen under physiological conditions after feeding, the increase in LES pressure produced by feeding cannot be explained exclusively on the basis of endogenous gastrin release.", "PMID": 955342}
{"id": "pubmed23n0032_1620", "title": "Characterization of a hyperactive segment at the rectosigmoid junction.", "content": "During a study of intraluminal motor patterns of the colon and rectum, spontaneous wave activity of a continuous complex type was observed at the rectosigmoid junction in constipated subjects. To assess the frequency and characteristics of this hyperactive segment, 36 subjects with colonic motor disorders and 12 healthy controls were studied. Eighteen of 24 patients with constipation (75%) and 1 of 7 subjects with asymptomatic diverticulosis exhibited a persistent hyperactive segment at the rectosigmoid junction. Neither secretin nor cholecystokinin influenced the wave activity of the hyperactive segment. In contrast, atropine and glucagon inhibited markedly all wave activity and decreased the motility index of this segment significantly, suggesting overactivity of the muscarinic effector cells. It is concluded that a segmental area of overactivity exists at the rectosigmoid junction in most constipated subjects regardless of their underlying disorders.", "contents": "Characterization of a hyperactive segment at the rectosigmoid junction. During a study of intraluminal motor patterns of the colon and rectum, spontaneous wave activity of a continuous complex type was observed at the rectosigmoid junction in constipated subjects. To assess the frequency and characteristics of this hyperactive segment, 36 subjects with colonic motor disorders and 12 healthy controls were studied. Eighteen of 24 patients with constipation (75%) and 1 of 7 subjects with asymptomatic diverticulosis exhibited a persistent hyperactive segment at the rectosigmoid junction. Neither secretin nor cholecystokinin influenced the wave activity of the hyperactive segment. In contrast, atropine and glucagon inhibited markedly all wave activity and decreased the motility index of this segment significantly, suggesting overactivity of the muscarinic effector cells. It is concluded that a segmental area of overactivity exists at the rectosigmoid junction in most constipated subjects regardless of their underlying disorders.", "PMID": 955344}
{"id": "pubmed23n0032_1621", "title": "Amylase isoenzyme clearances in normal subjects and in patients with acute pancreatitis.", "content": "Renal clearances of amylase isoenzymes, expressed as percentages of creatinine clearances, were determined in 20 normal subjects and in 8 patients with acute pancreatitis. The isoenzyme assay employed thin layer isoelectric focusing, starch iodine staining, and densitometry. Normal clearance of pancreatic-like amylase (mean +/- SE: 3.00 +/- 0.40%) was greater than the clearance of salivary-like amylase (0.51 +/- 0.06%) in ea ch individual (P less than 0.001). However, the amount of pancreatic amylase in the serum was not the major determinant of amylase clearance. Normal clearance of pancreatic-like amylase was significantly (P less than 0.001) less than the clearance of total serum amylase in acute pancreatitis (6.49 +/- 1.07%). In pancreatitis the clearance of pancreatic-like amylase (7.29 +/- 1.19%) and the clearance of salivary-like amylase (4.55 +/- 1.02%) were both elevated compared to normal (P less than 0.001). These findings indicate that the increased clearance of amylase in pancreatitis results from a change in renal function rather than a change in serum amylase. Renal changes not reflected by increased serum creatinine or more than mild proteinuria may be manifestations of the systemic toxicity of acute pancreatitis.", "contents": "Amylase isoenzyme clearances in normal subjects and in patients with acute pancreatitis. Renal clearances of amylase isoenzymes, expressed as percentages of creatinine clearances, were determined in 20 normal subjects and in 8 patients with acute pancreatitis. The isoenzyme assay employed thin layer isoelectric focusing, starch iodine staining, and densitometry. Normal clearance of pancreatic-like amylase (mean +/- SE: 3.00 +/- 0.40%) was greater than the clearance of salivary-like amylase (0.51 +/- 0.06%) in ea ch individual (P less than 0.001). However, the amount of pancreatic amylase in the serum was not the major determinant of amylase clearance. Normal clearance of pancreatic-like amylase was significantly (P less than 0.001) less than the clearance of total serum amylase in acute pancreatitis (6.49 +/- 1.07%). In pancreatitis the clearance of pancreatic-like amylase (7.29 +/- 1.19%) and the clearance of salivary-like amylase (4.55 +/- 1.02%) were both elevated compared to normal (P less than 0.001). These findings indicate that the increased clearance of amylase in pancreatitis results from a change in renal function rather than a change in serum amylase. Renal changes not reflected by increased serum creatinine or more than mild proteinuria may be manifestations of the systemic toxicity of acute pancreatitis.", "PMID": 955345}
{"id": "pubmed23n0032_1622", "title": "Serum amylase determinations and amylase to creatinine clearance ratios in patients with chronic renal insufficiency.", "content": "Patients with severe chronic renal failure may have significant hyperamylasemia in the absence of clinical symptoms or signs of acute pancreatitis. Amylase to creatinine clearance (CA/CC) ratios were usually elevated in patients with chronic renal failure and were not helpful in evaluating the possibility of acute pancreatitis. The mean amylase to creatinine clearance ratio for the controls with normal renal function was 1.24 +/- 0.13. In patients with chronic renal failure, it was 3.17 +/- 0.42 (P less than 0.001). Serum amylase isoenzyme patterns revealed no difference in salivary to pancreatic isoenzyme ratios between normals (1.04 +/- 0.12) and patients with severe renal insufficiency without evidence of pancreatic disease (1.07 +/- 0.13). The isoenzymes were helpful in excluding the diagnosis of pancreatic in 1 renal failure patient whose hyperamylasemia was primarily salivary in origin and in confirming the diagnosis in another who had only a pancreatic band.", "contents": "Serum amylase determinations and amylase to creatinine clearance ratios in patients with chronic renal insufficiency. Patients with severe chronic renal failure may have significant hyperamylasemia in the absence of clinical symptoms or signs of acute pancreatitis. Amylase to creatinine clearance (CA/CC) ratios were usually elevated in patients with chronic renal failure and were not helpful in evaluating the possibility of acute pancreatitis. The mean amylase to creatinine clearance ratio for the controls with normal renal function was 1.24 +/- 0.13. In patients with chronic renal failure, it was 3.17 +/- 0.42 (P less than 0.001). Serum amylase isoenzyme patterns revealed no difference in salivary to pancreatic isoenzyme ratios between normals (1.04 +/- 0.12) and patients with severe renal insufficiency without evidence of pancreatic disease (1.07 +/- 0.13). The isoenzymes were helpful in excluding the diagnosis of pancreatic in 1 renal failure patient whose hyperamylasemia was primarily salivary in origin and in confirming the diagnosis in another who had only a pancreatic band.", "PMID": 955346}
{"id": "pubmed23n0032_1623", "title": "Stimulation of DNA synthesis by big and little gastrin (G-34 and G-17).", "content": "Fasted rats were injected six times over a 48-hr period with G-17 I, G-17 II, G-34 II in doses doubling from 3.38 nmoles per kg to 54 nmoles per kg. Twelve rats were studied at each dose. NaCl-injected animals were used as controls. The rats were killed and the in vitro incorporation of [3H]thymidine into DNA as well as the total DNA content of the mucosa of the duodenum and oxyntic gland area of the stomach were determined. All gastrins, as well as pentagastrin, stimulated DNA synthesis. Peak stimulation occurred at 13.5 nmoles per kg for G-17 I and G-17 II and at 6.75 nmoles per kg for G-34 II. Pentagastrin's peak trophic effect was produced by 325 nmoles per kg. In order to compare the efficacies of the various types of gastrins, 60 rats were divided into groups of 12. One group received saline and the other four groups received the maximally effective dose of one of the four types of gastrins. The responses to each of the gastrins were not significantly different, and DNA synthesis was doubled in each tissue. Total DNA content increased slightly, but significantly, in response to each gastrin. Several conclusions can be drawn from these data: (1) G-17 and G-34 possess trophic activity; (2) the efficacies of pentagastrin, G-17 I, G-17 II, and G-34 II for the stimulation of DNA synthesis are not significantly different; (3) sulfation of G-17 has no significant effect on its trophic activity; (4) based on the effects of exogenous molar doses and their respective half-lives, both G-17 and G-34 would be expected to contribute to the trophic effect of endogenous gastrin; and (5) G-17 and G-34 are at least as effective in stimulating DNA synthesis as they are in stimulating gastric acid secretion.", "contents": "Stimulation of DNA synthesis by big and little gastrin (G-34 and G-17). Fasted rats were injected six times over a 48-hr period with G-17 I, G-17 II, G-34 II in doses doubling from 3.38 nmoles per kg to 54 nmoles per kg. Twelve rats were studied at each dose. NaCl-injected animals were used as controls. The rats were killed and the in vitro incorporation of [3H]thymidine into DNA as well as the total DNA content of the mucosa of the duodenum and oxyntic gland area of the stomach were determined. All gastrins, as well as pentagastrin, stimulated DNA synthesis. Peak stimulation occurred at 13.5 nmoles per kg for G-17 I and G-17 II and at 6.75 nmoles per kg for G-34 II. Pentagastrin's peak trophic effect was produced by 325 nmoles per kg. In order to compare the efficacies of the various types of gastrins, 60 rats were divided into groups of 12. One group received saline and the other four groups received the maximally effective dose of one of the four types of gastrins. The responses to each of the gastrins were not significantly different, and DNA synthesis was doubled in each tissue. Total DNA content increased slightly, but significantly, in response to each gastrin. Several conclusions can be drawn from these data: (1) G-17 and G-34 possess trophic activity; (2) the efficacies of pentagastrin, G-17 I, G-17 II, and G-34 II for the stimulation of DNA synthesis are not significantly different; (3) sulfation of G-17 has no significant effect on its trophic activity; (4) based on the effects of exogenous molar doses and their respective half-lives, both G-17 and G-34 would be expected to contribute to the trophic effect of endogenous gastrin; and (5) G-17 and G-34 are at least as effective in stimulating DNA synthesis as they are in stimulating gastric acid secretion.", "PMID": 955347}
{"id": "pubmed23n0032_1624", "title": "Distribution of excitatory vagal fibers in canine gastric wall to control motility.", "content": "The distribution of cholinergic excitatory neurones in the canine gastric wall was studied by recording electrical and mechanical responses to electrical stimulation of the nerves of Latarjet and close intraarterial perfusions of drugs which stimulate nerve and muscle. Electrical stimulation of the anterior nerve of Latarjet produced contractile activity on the anterior side, followed rapidly by activity on the posterior side and vice versa, indicating that the axons of the neurones from each side crossed over to the other. This was not due to the propagation or conduction of electrical response activity, because electrical response activity and contractions induced locally by acetylcholine given into a small artery supplying a segment of stomach did not propagate. The cross-over innervation and the direct innervation were both segmental, in that the response to nerve stimulation dropped out in segments as the branches of the nerves of Latarjet were cut successively. Mainly, preganglionic axons crossed over from the anterior side to the posterior side, but both pre- and postganglionic axons crossed over from the posterior side. This was demonstrated by observing the spread of electrical and mechanical responses, or lack of it, in response to local injections of acetylcholine and dimethylpeprazinium. The study shows that there is a segmental distribution of excitatory neurones in the gastric wall which is appropriate to produce a ring of contraction to control gastric emptying and mixing.", "contents": "Distribution of excitatory vagal fibers in canine gastric wall to control motility. The distribution of cholinergic excitatory neurones in the canine gastric wall was studied by recording electrical and mechanical responses to electrical stimulation of the nerves of Latarjet and close intraarterial perfusions of drugs which stimulate nerve and muscle. Electrical stimulation of the anterior nerve of Latarjet produced contractile activity on the anterior side, followed rapidly by activity on the posterior side and vice versa, indicating that the axons of the neurones from each side crossed over to the other. This was not due to the propagation or conduction of electrical response activity, because electrical response activity and contractions induced locally by acetylcholine given into a small artery supplying a segment of stomach did not propagate. The cross-over innervation and the direct innervation were both segmental, in that the response to nerve stimulation dropped out in segments as the branches of the nerves of Latarjet were cut successively. Mainly, preganglionic axons crossed over from the anterior side to the posterior side, but both pre- and postganglionic axons crossed over from the posterior side. This was demonstrated by observing the spread of electrical and mechanical responses, or lack of it, in response to local injections of acetylcholine and dimethylpeprazinium. The study shows that there is a segmental distribution of excitatory neurones in the gastric wall which is appropriate to produce a ring of contraction to control gastric emptying and mixing.", "PMID": 955348}
{"id": "pubmed23n0032_1625", "title": "Population kinetics of antral gastrin cells in the mouse.", "content": "The proliferation of antral gastrin cells has been studied in adult mice by combining their immunoperoxidase identification on semithin Epon sections with radioautography after single or multiple injections of [3H]thymidine. After a single injection, nuclear uptake of [3H]thymidine was observed in 0.25% of the well-differentiated gastrin cells. Ten days after six successive injections of the precursor, the labeling index reached 4.5%. This indicated that the renewal of the gastrin cell population was very slow. The turnover time was roughly estimated at 2 to 4 months. Analysis of the labeling index curves obtained during and after the administration of [3H]thymidine confirmed that the majority of the gastrin cells were renewed through replication of other gastrin cells. However, differentiation of a fraction of this gastrin cell population from \"other\" cells in the antrum could not be excluded.", "contents": "Population kinetics of antral gastrin cells in the mouse. The proliferation of antral gastrin cells has been studied in adult mice by combining their immunoperoxidase identification on semithin Epon sections with radioautography after single or multiple injections of [3H]thymidine. After a single injection, nuclear uptake of [3H]thymidine was observed in 0.25% of the well-differentiated gastrin cells. Ten days after six successive injections of the precursor, the labeling index reached 4.5%. This indicated that the renewal of the gastrin cell population was very slow. The turnover time was roughly estimated at 2 to 4 months. Analysis of the labeling index curves obtained during and after the administration of [3H]thymidine confirmed that the majority of the gastrin cells were renewed through replication of other gastrin cells. However, differentiation of a fraction of this gastrin cell population from \"other\" cells in the antrum could not be excluded.", "PMID": 955349}
{"id": "pubmed23n0032_1626", "title": "Altered small bowel propulsion associated with parasitism.", "content": "Experiments were designed to test the hypothesis that alterations of intestinal motility accompany enteric helminth infections. Motility was measured in rats during the intestinal phase of infection with the nematode Trichinella spiralis by following the transit of radioactive chromium through the gut. Intraduodenal catheters were surgically implanted in rats. One week after the operation one group of animals was infected with 8 x 10(3) T. spiralis larvae and a second group was infected with 16 x10(3) larvae. A third group of uninfected rats served as controls. Three, four, or five days postinfection, Na2 51CrO4 was injected through the catheter into the duodenum or fasted animals and its propulsion through the small bowel was allowed to progress for 15 min. The distribution of radioactivity throughout the small intestine and cecum was then plotted as a function of gut length. Intestinal transit was increased significantly by infection. The leading edge of radioactivity transversed 100% of the gut length in infected rats and 70% in controls. The amount of injected radioactivity transversing the midpoint of the small intestine was estimated by regression equations to be 38% of the injected dose for control rats and 59 and 57% for infected groups. Increased propulsive activity in parasitized rats was associated with inflammatory changes and a significant reduction in disaccharidase levels in the gut mucosa. The latter conditions were most marked in the proximal small bowel where the majority of worms resided.", "contents": "Altered small bowel propulsion associated with parasitism. Experiments were designed to test the hypothesis that alterations of intestinal motility accompany enteric helminth infections. Motility was measured in rats during the intestinal phase of infection with the nematode Trichinella spiralis by following the transit of radioactive chromium through the gut. Intraduodenal catheters were surgically implanted in rats. One week after the operation one group of animals was infected with 8 x 10(3) T. spiralis larvae and a second group was infected with 16 x10(3) larvae. A third group of uninfected rats served as controls. Three, four, or five days postinfection, Na2 51CrO4 was injected through the catheter into the duodenum or fasted animals and its propulsion through the small bowel was allowed to progress for 15 min. The distribution of radioactivity throughout the small intestine and cecum was then plotted as a function of gut length. Intestinal transit was increased significantly by infection. The leading edge of radioactivity transversed 100% of the gut length in infected rats and 70% in controls. The amount of injected radioactivity transversing the midpoint of the small intestine was estimated by regression equations to be 38% of the injected dose for control rats and 59 and 57% for infected groups. Increased propulsive activity in parasitized rats was associated with inflammatory changes and a significant reduction in disaccharidase levels in the gut mucosa. The latter conditions were most marked in the proximal small bowel where the majority of worms resided.", "PMID": 955350}
{"id": "pubmed23n0032_1627", "title": "Hepatitis B surface antigen carrier state in hepatosplenic schistosomiasis.", "content": "The prevalence of hepatitis B surface antigen (HBsAg) was studied in 103 cases of hepatosplenic schistosomiasis (HSS), 134 control cases with a variety of illnesses including hepatointestinal schistosomiasis, and 600 blood donors, in an area endemic for both schisfosomiasis and viral hepatitis. The patients with HSS proved to be persistent carriers for HBsAg in a significantly higher proportion than the other two groups of cases. The HSS cases who were carriers of HBsAg had more clinical signs of chronic liver disease and strikingly more chronic inflammation of the portal spaces on liver biopsy. It is suggested that abnormal immunological responses in patients with HSS makes them more susceptible to become carriers of HBsAg and that the addition of this injurious factor makes their basic disease worse, and may be responsible for the development of cirrhosis in some cases.", "contents": "Hepatitis B surface antigen carrier state in hepatosplenic schistosomiasis. The prevalence of hepatitis B surface antigen (HBsAg) was studied in 103 cases of hepatosplenic schistosomiasis (HSS), 134 control cases with a variety of illnesses including hepatointestinal schistosomiasis, and 600 blood donors, in an area endemic for both schisfosomiasis and viral hepatitis. The patients with HSS proved to be persistent carriers for HBsAg in a significantly higher proportion than the other two groups of cases. The HSS cases who were carriers of HBsAg had more clinical signs of chronic liver disease and strikingly more chronic inflammation of the portal spaces on liver biopsy. It is suggested that abnormal immunological responses in patients with HSS makes them more susceptible to become carriers of HBsAg and that the addition of this injurious factor makes their basic disease worse, and may be responsible for the development of cirrhosis in some cases.", "PMID": 955351}
{"id": "pubmed23n0032_1628", "title": "Necrotizing granulomatous vasculitis (allergic granulomatosis) of the gallbladder.", "content": "A case of necrotizing granulomatous vasculitis limited to the gallbladder and manifesting as acute cholecystitis is presented. The different types of vasculitis are discussed and the characteristics of necrotizing granulomatous vasculitis (allergic granulomatosis) are described.", "contents": "Necrotizing granulomatous vasculitis (allergic granulomatosis) of the gallbladder. A case of necrotizing granulomatous vasculitis limited to the gallbladder and manifesting as acute cholecystitis is presented. The different types of vasculitis are discussed and the characteristics of necrotizing granulomatous vasculitis (allergic granulomatosis) are described.", "PMID": 955352}
{"id": "pubmed23n0032_1629", "title": "Double duodenal diaphragms in an adult: endoscopic, radiographic, and operative findings.", "content": "Hypotonic duodenography, duodenoscopy, and operative exploration were performed in an adult with double duodenal webs and symptoms suggesting gastric outlet obstruction. The spectrum of diagnostic features associated with this rare entity is described in this report.", "contents": "Double duodenal diaphragms in an adult: endoscopic, radiographic, and operative findings. Hypotonic duodenography, duodenoscopy, and operative exploration were performed in an adult with double duodenal webs and symptoms suggesting gastric outlet obstruction. The spectrum of diagnostic features associated with this rare entity is described in this report.", "PMID": 955353}
{"id": "pubmed23n0032_1630", "title": "Ileojejunitis in a pair of identical twins.", "content": "A pair of identical twin brothers with identical white and red blood cell antigens and diffuse ileojejunitis is reported. One brother initially presented with granulomatous gastritis, presumably Crohn's disease of the stomach. The relationship of diffuse ileojejunitis to Crohn's disease and the suspected genetic predisposition to develop Crohn's disease are briefly discussed.", "contents": "Ileojejunitis in a pair of identical twins. A pair of identical twin brothers with identical white and red blood cell antigens and diffuse ileojejunitis is reported. One brother initially presented with granulomatous gastritis, presumably Crohn's disease of the stomach. The relationship of diffuse ileojejunitis to Crohn's disease and the suspected genetic predisposition to develop Crohn's disease are briefly discussed.", "PMID": 955354}
{"id": "pubmed23n0032_1631", "title": "Progressive intrahepatic cholestasis of infancy and childhood. A clinicopathological study of patient surviving to the age of 18 years.", "content": "A patient who developed chronic cholestatic jaundice during the 1st year of life and eventually died of liver cell failure at the age of 18 years is described. During the terminal illness Kayser-Fleischer-like rings were observed and the serum concentrations of total copper and ceruloplasmin were elevated. At autopsy, a mixed macronodular and micronodular cirrhosis was found and cholangiography and dissection of bile ducts revealed no obstructive lesion of the biliary tract. There was no family history of hepatobiliary disease. Liver biopsies obtained at the ages of 5 and 7 years showed accumulation of bile droplets in hepatocytes, normal-appearing bile ducts, no significant fibrosis, and intact lobular architecture. Striking features of the terminal cirrhosis were the presence of Mallory bodies and a marked excess of copper in the liver (2,175 mug per g dry weight). The latter two findings, as well as the elevated serum concentrations of total copper and ceruloplasmin, may be attributable to chronic cholestasis per se. This study emphasizes the clinical and therapeutic problems posed by chronic cholestasis of unknown etiology in childhood.", "contents": "Progressive intrahepatic cholestasis of infancy and childhood. A clinicopathological study of patient surviving to the age of 18 years. A patient who developed chronic cholestatic jaundice during the 1st year of life and eventually died of liver cell failure at the age of 18 years is described. During the terminal illness Kayser-Fleischer-like rings were observed and the serum concentrations of total copper and ceruloplasmin were elevated. At autopsy, a mixed macronodular and micronodular cirrhosis was found and cholangiography and dissection of bile ducts revealed no obstructive lesion of the biliary tract. There was no family history of hepatobiliary disease. Liver biopsies obtained at the ages of 5 and 7 years showed accumulation of bile droplets in hepatocytes, normal-appearing bile ducts, no significant fibrosis, and intact lobular architecture. Striking features of the terminal cirrhosis were the presence of Mallory bodies and a marked excess of copper in the liver (2,175 mug per g dry weight). The latter two findings, as well as the elevated serum concentrations of total copper and ceruloplasmin, may be attributable to chronic cholestasis per se. This study emphasizes the clinical and therapeutic problems posed by chronic cholestasis of unknown etiology in childhood.", "PMID": 955355}
{"id": "pubmed23n0032_1632", "title": "Lesions brought on by vomiting: the effect of hiatus hernia of the site of injury.", "content": "From an extensive review of the published cases of lesions of the esophagus and stomach brought about by vomiting and a supplemental review of local experience with the Mallory-Weiss syndrome, the influence of hiatus hernia on the lesion site can now be defined. Mallory-Weiss lesions located in the gastric cardia are commonly associated with hiatus hernia. By contrast, those occurring in the distal esophagus and those that overlie the cardioesophageal junction are only rarely associated with hernia. The Boerhaave lesion occurs most probably in the absence of hiatus hernia and in 2 of 3 previously reported cases wherein a hernia was present, a combined gastroesophageal rupture occurred. A newly recognized postemetic form of gastric rupture is clearly distinguished from other causes of this lesion and its characteristic location is described. A unifying hypothesis is proposed in which the location of lesions occurring during emesis is determined by the presence of a hiatus hernia during retching.", "contents": "Lesions brought on by vomiting: the effect of hiatus hernia of the site of injury. From an extensive review of the published cases of lesions of the esophagus and stomach brought about by vomiting and a supplemental review of local experience with the Mallory-Weiss syndrome, the influence of hiatus hernia on the lesion site can now be defined. Mallory-Weiss lesions located in the gastric cardia are commonly associated with hiatus hernia. By contrast, those occurring in the distal esophagus and those that overlie the cardioesophageal junction are only rarely associated with hernia. The Boerhaave lesion occurs most probably in the absence of hiatus hernia and in 2 of 3 previously reported cases wherein a hernia was present, a combined gastroesophageal rupture occurred. A newly recognized postemetic form of gastric rupture is clearly distinguished from other causes of this lesion and its characteristic location is described. A unifying hypothesis is proposed in which the location of lesions occurring during emesis is determined by the presence of a hiatus hernia during retching.", "PMID": 955356}
{"id": "pubmed23n0032_1633", "title": "[Stars in mammography--cancers and radial scars (author's transl)].", "content": "Among 50 little starlike shadows in about 13,500 mammograms during the years 1973-1975 there were seven pictures proved histologically not to be cancers but \"radial scars\". We found no X-ray features to differentiate between scars and cancers. The uniform histologic picture of a centrally placed elastotic periductal scar with radial processes allowed however the exact differentiation. Especially significant for these radial scars is the storage of elastotic amorphous material around obliterated central ducts. Cystic atrophy or highgrade papillary epithelial proliferation in the surroundings may be combined with the central scar. Cancerous growth in this area is diagnosed without difficulty by the infiltration beyond the border of the star area.", "contents": "[Stars in mammography--cancers and radial scars (author's transl)]. Among 50 little starlike shadows in about 13,500 mammograms during the years 1973-1975 there were seven pictures proved histologically not to be cancers but \"radial scars\". We found no X-ray features to differentiate between scars and cancers. The uniform histologic picture of a centrally placed elastotic periductal scar with radial processes allowed however the exact differentiation. Especially significant for these radial scars is the storage of elastotic amorphous material around obliterated central ducts. Cystic atrophy or highgrade papillary epithelial proliferation in the surroundings may be combined with the central scar. Cancerous growth in this area is diagnosed without difficulty by the infiltration beyond the border of the star area.", "PMID": 955361}
{"id": "pubmed23n0032_1634", "title": "[Negative smear in cervical carcinoma in situ (author's transl)].", "content": "In 100 patients which had shown ca-in-situ in the conization specimen during a preoperative colposcopic and cytologic investigation separate smears were taken from the anterior lip, the posterior lip and from the cervical canal. The three slides in each case have been examined to determine the frequency of negative smears, especially those of the endocervix, and to evaluate any relationship between those findings and the localization and the extent of the lesion. 22% of these patients belonged to the age group of 20-29 years, 3% were older than 60 years. Depending on the microscopic description we differentiated between small, circumscript lesions (17 cases), intracervical lesions (24) and extended lesions (59). In only 49% of the cases all three smears had been positive (Pap III-V). In 23% of the cases the endocervical smear was negative. In small, circumscript lesions at least one negative smear was found in 80% of the cases. In the extended lesions one third had at least one negative slide. In 25% of the patients with an intracervical lesion the endocervical smear was negative. Re-evaluation of the negative smears has shown an examiner's failure in about one third of these, due to several factors. In 24 patients the triple cervical smear technique had been applied at least twice. One third of the slides exhibited differences concerning negativity and positivity between the first and the second preparation.", "contents": "[Negative smear in cervical carcinoma in situ (author's transl)]. In 100 patients which had shown ca-in-situ in the conization specimen during a preoperative colposcopic and cytologic investigation separate smears were taken from the anterior lip, the posterior lip and from the cervical canal. The three slides in each case have been examined to determine the frequency of negative smears, especially those of the endocervix, and to evaluate any relationship between those findings and the localization and the extent of the lesion. 22% of these patients belonged to the age group of 20-29 years, 3% were older than 60 years. Depending on the microscopic description we differentiated between small, circumscript lesions (17 cases), intracervical lesions (24) and extended lesions (59). In only 49% of the cases all three smears had been positive (Pap III-V). In 23% of the cases the endocervical smear was negative. In small, circumscript lesions at least one negative smear was found in 80% of the cases. In the extended lesions one third had at least one negative slide. In 25% of the patients with an intracervical lesion the endocervical smear was negative. Re-evaluation of the negative smears has shown an examiner's failure in about one third of these, due to several factors. In 24 patients the triple cervical smear technique had been applied at least twice. One third of the slides exhibited differences concerning negativity and positivity between the first and the second preparation.", "PMID": 955363}
{"id": "pubmed23n0032_1635", "title": "[Prognostic factors in early endometrial carcinoma (FIGO stage I)].", "content": "The evidence concerning the most important factors related to prognosis in endometrial carcinoma stage I has been reviewed in 500 cases. Histologic differentiation of the tumor and depth of myometrial invasion have been found to be definitely correlated with survival, whereas uterine site in stage I lesions treated by hysterectomy shows no correlation between depth of uterine sounding and survival. Obesity and hypertension influence outcome in cases treated by radiation alone.", "contents": "[Prognostic factors in early endometrial carcinoma (FIGO stage I)]. The evidence concerning the most important factors related to prognosis in endometrial carcinoma stage I has been reviewed in 500 cases. Histologic differentiation of the tumor and depth of myometrial invasion have been found to be definitely correlated with survival, whereas uterine site in stage I lesions treated by hysterectomy shows no correlation between depth of uterine sounding and survival. Obesity and hypertension influence outcome in cases treated by radiation alone.", "PMID": 955364}
{"id": "pubmed23n0032_1636", "title": "[A new obstetric forceps: the Bamberger divergent forceps (author's transl)].", "content": "A new obstetric forceps is presented which has been used since April 1975 in 52 cases for outlet forceps deliveries. The model is a divergent forceps with the rotational axis in the posterior 1/3. The forceps is applied as usual. The shanks do not cross. The distance between the blades is variable by a mechanism in the handle with an automatic stopping mechanism. Pressure on the fetal head is largely avoided. Our experience has shown that this forceps has advantages over the types of forceps in common usage.", "contents": "[A new obstetric forceps: the Bamberger divergent forceps (author's transl)]. A new obstetric forceps is presented which has been used since April 1975 in 52 cases for outlet forceps deliveries. The model is a divergent forceps with the rotational axis in the posterior 1/3. The forceps is applied as usual. The shanks do not cross. The distance between the blades is variable by a mechanism in the handle with an automatic stopping mechanism. Pressure on the fetal head is largely avoided. Our experience has shown that this forceps has advantages over the types of forceps in common usage.", "PMID": 955365}
{"id": "pubmed23n0032_1637", "title": "[Isolated tortion of the fallopian tube. Report on a recurrent tortion of a hematosalpinx due to genital tuberculosis (author's transl)].", "content": "A case of recurrent contralateral isolated tortion of tubercle hematosalpinx four years after the first event is reported. The clinical picture and the pathogenesis of the isolated tortion of the fallopian tube is discussed. This disease is considered to be rare and the etiology of isolated tortion of the tube is largely unknown. As shown in this case, tortion of the tube can occur in cases of genital tuberculosis. Since acute bleeding into the fallopian tube may be pathognomonic for tortion this factor should be considered when the findings at laparotomy are interpreted. Tortion of a hematosalpinx requires additional diagnostic measures to detect the underlying disease.", "contents": "[Isolated tortion of the fallopian tube. Report on a recurrent tortion of a hematosalpinx due to genital tuberculosis (author's transl)]. A case of recurrent contralateral isolated tortion of tubercle hematosalpinx four years after the first event is reported. The clinical picture and the pathogenesis of the isolated tortion of the fallopian tube is discussed. This disease is considered to be rare and the etiology of isolated tortion of the tube is largely unknown. As shown in this case, tortion of the tube can occur in cases of genital tuberculosis. Since acute bleeding into the fallopian tube may be pathognomonic for tortion this factor should be considered when the findings at laparotomy are interpreted. Tortion of a hematosalpinx requires additional diagnostic measures to detect the underlying disease.", "PMID": 955367}
{"id": "pubmed23n0032_1638", "title": "[Observations concerning the moment of delivery in 144 gestational diabetics (author's transl)].", "content": "Report is made, in a comparative examination, about infant mortality and early morbidity, as well as about several further perinatal criteria in gestational diabetics, 85 of whom were delivered before/on the calculated date and 59 after the term. With the exception of weight and length of the newborn, no significant differences are occurring in any of the symptoms selected. The same situation appears in a control group consisting of people with healthy metabolism. Conclusion is made thereof that gestational diabetes per se does not represent an indication to terminate pregnancy at any rate on the calculated date.", "contents": "[Observations concerning the moment of delivery in 144 gestational diabetics (author's transl)]. Report is made, in a comparative examination, about infant mortality and early morbidity, as well as about several further perinatal criteria in gestational diabetics, 85 of whom were delivered before/on the calculated date and 59 after the term. With the exception of weight and length of the newborn, no significant differences are occurring in any of the symptoms selected. The same situation appears in a control group consisting of people with healthy metabolism. Conclusion is made thereof that gestational diabetes per se does not represent an indication to terminate pregnancy at any rate on the calculated date.", "PMID": 955368}
{"id": "pubmed23n0032_1639", "title": "[Experiments of electro-acupuncture-analgesia during delivery (author's transl)].", "content": "We report about 20 patients who underwent electroacupuncture therapy during delivery with intent to reduce labor pains. Acupuncture steel needles situated in the thoracal, lumbal, sacral region and in the region above the symphysis were stimulated by a rectangular impulse current. A good effect for the reduction of labor pains could be observed in 5 cases, a moderate one in 6 cases. An absolute analgetic effect could not be achieved in any case, and in 9 cases no analgetic effect of acupuncture therapy on labor pains was visible. Acupuncture had no influence on labor or fetal heart rate, monitored by cardiotocography.", "contents": "[Experiments of electro-acupuncture-analgesia during delivery (author's transl)]. We report about 20 patients who underwent electroacupuncture therapy during delivery with intent to reduce labor pains. Acupuncture steel needles situated in the thoracal, lumbal, sacral region and in the region above the symphysis were stimulated by a rectangular impulse current. A good effect for the reduction of labor pains could be observed in 5 cases, a moderate one in 6 cases. An absolute analgetic effect could not be achieved in any case, and in 9 cases no analgetic effect of acupuncture therapy on labor pains was visible. Acupuncture had no influence on labor or fetal heart rate, monitored by cardiotocography.", "PMID": 955369}
{"id": "pubmed23n0032_1640", "title": "Rehabilitation performance and adjustment in stroke patients: a study of social class factors.", "content": "In a prospective follow-up study comparing the adjustment of hemiplegics in rehabilitation programs in a public institution (N = 100) and a private university facility (N = 105) measures were taken one month after admission and 18-24 months after discharge. Initially, the groups differed in social profiles (social class, ethnicity, marital status), incidence of secondary medical conditions and attitudes, including expectancies and F scale and sick role questionnaire. They did not differ in performance and motivation on the programs. At follow-up treatment. Many more went to nursing homes. Implications for rehabilitation programs are presented.", "contents": "Rehabilitation performance and adjustment in stroke patients: a study of social class factors. In a prospective follow-up study comparing the adjustment of hemiplegics in rehabilitation programs in a public institution (N = 100) and a private university facility (N = 105) measures were taken one month after admission and 18-24 months after discharge. Initially, the groups differed in social profiles (social class, ethnicity, marital status), incidence of secondary medical conditions and attitudes, including expectancies and F scale and sick role questionnaire. They did not differ in performance and motivation on the programs. At follow-up treatment. Many more went to nursing homes. Implications for rehabilitation programs are presented.", "PMID": 955401}
{"id": "pubmed23n0032_1641", "title": "Characterization of T4 mutants that partially suppress the inability of T4rII to grow in Lambda lysogens.", "content": "In the course of isolating viable T4 deletions that affect plaque morphology (HOMYK and WEIL 1974), two closely linked point mutant, sip1 and sip2, were obtained. They map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rII mutants for growth in lambda lysogens. The characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by FREEDMAN and BRENNER(1972). In addition, D. Hall (personal communication) has shown that sip1 and sip2 are similar to the mutant farP85, which affects the regulation of a number of early genes (Chace and Hall 1975).--Sip suppression of rII mutants can be demonstrated in one-step growth experiments, even when both rII genes are completely deleted. This indicates that sip mutants do not simply reduce the level of rII gene products required for growth in a lambda lysogen. Instead, they alter the growth cycle so as to partially circumvent the need for any rII products.--Mutations at two other sites, designated L1 and L2, reverse the poor phage growth caused by sip and, in the one case tested, reverse the rII-suppressing ability of sip.", "contents": "Characterization of T4 mutants that partially suppress the inability of T4rII to grow in Lambda lysogens. In the course of isolating viable T4 deletions that affect plaque morphology (HOMYK and WEIL 1974), two closely linked point mutant, sip1 and sip2, were obtained. They map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rII mutants for growth in lambda lysogens. The characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by FREEDMAN and BRENNER(1972). In addition, D. Hall (personal communication) has shown that sip1 and sip2 are similar to the mutant farP85, which affects the regulation of a number of early genes (Chace and Hall 1975).--Sip suppression of rII mutants can be demonstrated in one-step growth experiments, even when both rII genes are completely deleted. This indicates that sip mutants do not simply reduce the level of rII gene products required for growth in a lambda lysogen. Instead, they alter the growth cycle so as to partially circumvent the need for any rII products.--Mutations at two other sites, designated L1 and L2, reverse the poor phage growth caused by sip and, in the one case tested, reverse the rII-suppressing ability of sip.", "PMID": 955402}
{"id": "pubmed23n0032_1642", "title": "Inducible monooxygenase activities and 3-methyl-cholanthrene-initiated tumorigenesis in mouse recombinant inbred sublines.", "content": "The induction of a certain group of hepatic monooxygenase activities by polycyclic aromatic compounds is regulated by the same locus or gene cluster controlling the formation of cytochrome P1-450 (P-448) in mice. Certain inbred strains of mice are \"responsive\" (Ahb) to such induction, whereas others are \"nonresponsive\" (Ahd). A pair of closely related sublines that differ with respect to the Ah locus (for aromatic hydrocarbon responsiveness) were used to identify or confirm the pleiotropic effects of this gene. The lines were derived by sibling-mating without selection from (C57L/J x AKR/J)F2 mice; the two sublines were separated at the F12 generation. Ten microsomal monooxygenase activities and one cytosol enzyme activity known to be associated with the Ah locus were similarly associated with cytochrome P1-450 formation in these recombinant inbred sublines as well. Nine additional hepatic monooxygenase activities studied were found not to be associated with the Ah locus; certain of these activities were increased slightly, following treatment of nonresponsive as well as responsive mice with polycyclic aromatic compounds. The Ahb-containing subline was highly susceptible to 3-methylcholanthrene-induced subcutaneous sarcomas, whereas the Ah-d-containing subline was relatively resistant. These results emphasize the potential importance of this particular enzyme for the study of coordinated regulation in mammals.", "contents": "Inducible monooxygenase activities and 3-methyl-cholanthrene-initiated tumorigenesis in mouse recombinant inbred sublines. The induction of a certain group of hepatic monooxygenase activities by polycyclic aromatic compounds is regulated by the same locus or gene cluster controlling the formation of cytochrome P1-450 (P-448) in mice. Certain inbred strains of mice are \"responsive\" (Ahb) to such induction, whereas others are \"nonresponsive\" (Ahd). A pair of closely related sublines that differ with respect to the Ah locus (for aromatic hydrocarbon responsiveness) were used to identify or confirm the pleiotropic effects of this gene. The lines were derived by sibling-mating without selection from (C57L/J x AKR/J)F2 mice; the two sublines were separated at the F12 generation. Ten microsomal monooxygenase activities and one cytosol enzyme activity known to be associated with the Ah locus were similarly associated with cytochrome P1-450 formation in these recombinant inbred sublines as well. Nine additional hepatic monooxygenase activities studied were found not to be associated with the Ah locus; certain of these activities were increased slightly, following treatment of nonresponsive as well as responsive mice with polycyclic aromatic compounds. The Ahb-containing subline was highly susceptible to 3-methylcholanthrene-induced subcutaneous sarcomas, whereas the Ah-d-containing subline was relatively resistant. These results emphasize the potential importance of this particular enzyme for the study of coordinated regulation in mammals.", "PMID": 955403}
{"id": "pubmed23n0032_1643", "title": "Simulation of X-linked selection in Drosophila.", "content": "The change in gene frequency for two X-linked mutants, gamma and omega, in a number of experiments was compared to that predicted from a genetic simulation program which utilized estimated differences in relative mating ability, fecundity, and viability. The simulation gave excellent predictions of gene frequency change even when experiments were started with different initial gene frequencies in the males and females or when the two loci were segregating simultaneously. The rate of elimination was slower when there were unequal initial gene frequencies than when males and females had equal initial gene frequencies. Simulation demonstrated that this was a general phenomenon when there is strong selection but that the opposite is true for weak selection. In two other experiments, the mating advantage of wild-type males was balanced by a fecundity advantage in mutant females. In all four replicates of both experiments, the mutant was maintained for several generations at the high initial frequency but then decreased quickly and was eliminated. Results obtained restarting one of these experiments with flies from a generation after the decline in gene frequency indicated that a linked gene and not frequency-dependent selection was responsible for the unpredictable gene-frequency change in the mutant. Using a least squares technique, it was found that a recessive fecundity locus 15 map units from the omega locus gave the best fit for both experiments.", "contents": "Simulation of X-linked selection in Drosophila. The change in gene frequency for two X-linked mutants, gamma and omega, in a number of experiments was compared to that predicted from a genetic simulation program which utilized estimated differences in relative mating ability, fecundity, and viability. The simulation gave excellent predictions of gene frequency change even when experiments were started with different initial gene frequencies in the males and females or when the two loci were segregating simultaneously. The rate of elimination was slower when there were unequal initial gene frequencies than when males and females had equal initial gene frequencies. Simulation demonstrated that this was a general phenomenon when there is strong selection but that the opposite is true for weak selection. In two other experiments, the mating advantage of wild-type males was balanced by a fecundity advantage in mutant females. In all four replicates of both experiments, the mutant was maintained for several generations at the high initial frequency but then decreased quickly and was eliminated. Results obtained restarting one of these experiments with flies from a generation after the decline in gene frequency indicated that a linked gene and not frequency-dependent selection was responsible for the unpredictable gene-frequency change in the mutant. Using a least squares technique, it was found that a recessive fecundity locus 15 map units from the omega locus gave the best fit for both experiments.", "PMID": 955404}
{"id": "pubmed23n0032_1644", "title": "The evolution of one- and two-locus systems.", "content": "Assuming age-independent fertilities and mortalities and random mating, continuous-time models for a monoecious population are investigated for weak selection. A single locus with multiple alleles and two alleles at each of two loci are considered. A slow-selection analysis of diallelic and multiallelic two-locus models with discrete nonoverlapping generations is also presented. The selective differences may be functions of genotypic frequencies, but their rate of change due to their explicit dependence on time (if any) must be at most of the second order in s, (i.e., O(s2), where s is the intensity of natural selection. Then, after several generations have elapsed, in the continuous time models the time-derivative of the deviations from Hardy-Weinberg proportions is of O(s2), and in the two-locus models the rate of change of the linkage disequilibrium is of O(s2). It follows that, if the rate of change of the genotypic fitnesses is smaller than second order in s (i.e., o(s2)), then to O(s2) the rate of change of the mean fitness of the population is equal to the genic variance. For a fixed value of s, however, no matter how small, the genic variance may occasionally be smaller in absolute value than the (possibly negative) lower order terms in the change in fitness, and hence the mean fitness may decrease. This happens if the allelic frequencies are changing extremely slowly, and hence occurs often very close to equilibrium. Some new expressions are derived for the change in mean fitness. It is shown that, with an error of O(s), the genotypic frequencies evolve as if the population were in Hardy-Weinberg proportions and linkage equilibrium. Thus, at least for the deterministic behaviour of one and two loci, deviations from random combination appear to have very little evolutionary significance.", "contents": "The evolution of one- and two-locus systems. Assuming age-independent fertilities and mortalities and random mating, continuous-time models for a monoecious population are investigated for weak selection. A single locus with multiple alleles and two alleles at each of two loci are considered. A slow-selection analysis of diallelic and multiallelic two-locus models with discrete nonoverlapping generations is also presented. The selective differences may be functions of genotypic frequencies, but their rate of change due to their explicit dependence on time (if any) must be at most of the second order in s, (i.e., O(s2), where s is the intensity of natural selection. Then, after several generations have elapsed, in the continuous time models the time-derivative of the deviations from Hardy-Weinberg proportions is of O(s2), and in the two-locus models the rate of change of the linkage disequilibrium is of O(s2). It follows that, if the rate of change of the genotypic fitnesses is smaller than second order in s (i.e., o(s2)), then to O(s2) the rate of change of the mean fitness of the population is equal to the genic variance. For a fixed value of s, however, no matter how small, the genic variance may occasionally be smaller in absolute value than the (possibly negative) lower order terms in the change in fitness, and hence the mean fitness may decrease. This happens if the allelic frequencies are changing extremely slowly, and hence occurs often very close to equilibrium. Some new expressions are derived for the change in mean fitness. It is shown that, with an error of O(s), the genotypic frequencies evolve as if the population were in Hardy-Weinberg proportions and linkage equilibrium. Thus, at least for the deterministic behaviour of one and two loci, deviations from random combination appear to have very little evolutionary significance.", "PMID": 955405}
{"id": "pubmed23n0032_1645", "title": "Remarks on the evolutionary effect of natural selection.", "content": "The so-called \"Fundamental Theorem of Natural Selectiion\", than the mean fitness of a population increases with time under natural selection, is known not to be true, as a mathematical theorem, when fitnesses depend on more than one locus. Although this observation may not have particular biological relevance, (so that mean fitness may well increase in the great majority of interesting situations), it does suggest that it is of interest to find an evolutionary result which is correct as a mathematical theorem, no matter how many loci are involved. The aim of the present note is to prove an evolutionary theorem relating to the variance in fitness, rather that the mean: this theorem is true for an arbitrary number of loci, as well as for arbitrary (fixed) fitness parameters and arbitrary linkage between loci. Connections are briefly discussed between this theorem and the principle of quasi-linkage equilibrium.", "contents": "Remarks on the evolutionary effect of natural selection. The so-called \"Fundamental Theorem of Natural Selectiion\", than the mean fitness of a population increases with time under natural selection, is known not to be true, as a mathematical theorem, when fitnesses depend on more than one locus. Although this observation may not have particular biological relevance, (so that mean fitness may well increase in the great majority of interesting situations), it does suggest that it is of interest to find an evolutionary result which is correct as a mathematical theorem, no matter how many loci are involved. The aim of the present note is to prove an evolutionary theorem relating to the variance in fitness, rather that the mean: this theorem is true for an arbitrary number of loci, as well as for arbitrary (fixed) fitness parameters and arbitrary linkage between loci. Connections are briefly discussed between this theorem and the principle of quasi-linkage equilibrium.", "PMID": 955406}
{"id": "pubmed23n0032_1646", "title": "[Mutagenic effect of small doses of diethylsulfate in laboratory mice studied by specific locus method].", "content": "Diethylsulphate solution was injected intraperitoneally twice a week to male mice B10.C3H having the wild-type colour at a 5 mg/kg dose. The treatment lasted 10 weeks, so that the total dose administered was 5-20 = 100 mg/kg. In fifth week after the end of the treatment these males were crossed to YT females homozygous for seven recessive mutant genes. In the control group all the F1 descendants were of wild-type colour, while in the experimental group among 5042 F1 mice derived from DES-treated spermatogonia there was one mutant d and 4 mosaics (3 with respect to the locus c and 1 with respect to the locusa). These results are interpreted as the evidence of the mutagenic effect of DES.", "contents": "[Mutagenic effect of small doses of diethylsulfate in laboratory mice studied by specific locus method]. Diethylsulphate solution was injected intraperitoneally twice a week to male mice B10.C3H having the wild-type colour at a 5 mg/kg dose. The treatment lasted 10 weeks, so that the total dose administered was 5-20 = 100 mg/kg. In fifth week after the end of the treatment these males were crossed to YT females homozygous for seven recessive mutant genes. In the control group all the F1 descendants were of wild-type colour, while in the experimental group among 5042 F1 mice derived from DES-treated spermatogonia there was one mutant d and 4 mosaics (3 with respect to the locus c and 1 with respect to the locusa). These results are interpreted as the evidence of the mutagenic effect of DES.", "PMID": 955407}
{"id": "pubmed23n0032_1647", "title": "[Possibility of using dermatoglyphic markers for assessment of genetic hypotheses in schizophrenia].", "content": "Dermatoglyphic alterations in schizophrenic patients are considered in virtue of literature data and the author's own investigations. The absence of obvious and regular difference between healthy people and schizophrenic patients in the incidence of finger and palm patterns is revealed. Only statistically insignificant tendency of different manifestations of some features in patients and healthy persons is observed. Skin patterns doubtly may be used as additional diagnostic paraclinical method and applied to work out genetic aspects of schizophrenia. The addiitonal investigations and particularly and the investigations of \"family forms\" of schizophrenia are suggested for suggested for complete solution of this problem.", "contents": "[Possibility of using dermatoglyphic markers for assessment of genetic hypotheses in schizophrenia]. Dermatoglyphic alterations in schizophrenic patients are considered in virtue of literature data and the author's own investigations. The absence of obvious and regular difference between healthy people and schizophrenic patients in the incidence of finger and palm patterns is revealed. Only statistically insignificant tendency of different manifestations of some features in patients and healthy persons is observed. Skin patterns doubtly may be used as additional diagnostic paraclinical method and applied to work out genetic aspects of schizophrenia. The addiitonal investigations and particularly and the investigations of \"family forms\" of schizophrenia are suggested for suggested for complete solution of this problem.", "PMID": 955408}
{"id": "pubmed23n0032_1648", "title": "[Polymorphism of near-centromeric heterochromatin in a population of normal humans].", "content": "Polymorphism of constitutive heterochromatin of metaphase chromosomes 1,9 and 16 in 6 normal humans (both men and women) in studied by means of C-staining. Only slight variability in size of C-blocks in one individium (from cell to cell) and a high frequency of C-chromation heteromorphism variants within the population studied is observed. 8 variants of C-heterochromatin (heteromorphic homologues) are found: 4 individuals had 1-2 variants and 1 individual - 3 variants. Quantitative analysis based on measurements of C-blocks confirmed the reliability of the existence of these variants.", "contents": "[Polymorphism of near-centromeric heterochromatin in a population of normal humans]. Polymorphism of constitutive heterochromatin of metaphase chromosomes 1,9 and 16 in 6 normal humans (both men and women) in studied by means of C-staining. Only slight variability in size of C-blocks in one individium (from cell to cell) and a high frequency of C-chromation heteromorphism variants within the population studied is observed. 8 variants of C-heterochromatin (heteromorphic homologues) are found: 4 individuals had 1-2 variants and 1 individual - 3 variants. Quantitative analysis based on measurements of C-blocks confirmed the reliability of the existence of these variants.", "PMID": 955410}
{"id": "pubmed23n0032_1649", "title": "[Stimulation of ribonuclease activity under the influence of nitrosoethylurea].", "content": "Nutrosoethyl urea-induced activation of alkaline ribonuclease was accelerated with the increase of the temperature from 22 to 32 degrees C. The reaction rate at 32 degrees C was more than 8 fold increased as compared with the control. Further increase of temperature to 42 degrees C decreased the stimulating effect of nitrosoethyl urea. The effect observed may be taken into consideration when studying a molecular mechanism of the effect of chemical mutagens on cell metabolism.", "contents": "[Stimulation of ribonuclease activity under the influence of nitrosoethylurea]. Nutrosoethyl urea-induced activation of alkaline ribonuclease was accelerated with the increase of the temperature from 22 to 32 degrees C. The reaction rate at 32 degrees C was more than 8 fold increased as compared with the control. Further increase of temperature to 42 degrees C decreased the stimulating effect of nitrosoethyl urea. The effect observed may be taken into consideration when studying a molecular mechanism of the effect of chemical mutagens on cell metabolism.", "PMID": 955411}
{"id": "pubmed23n0032_1650", "title": "[Comparison of the radiosensitivity of chromosomes during the S and G2 periods in regenerating rat liver cells].", "content": "Radiosensitivity of chromosomes at S and G2 periods was studied by means of autoradiography technique in cells or regenerating liver of rats treated with X-rays at the doses of 150 and 300 r. The experiments were made so that the interval between irradiation and mitosis in S and G2 cells was equal. The results have shown that under the experimental conditions there are significant differences in the yield of chromosome aberrations between the labelled and intact cells. This withnesses for the existence of differences in radiosensitivity of chromosomes at different periods of mitotic cycle.", "contents": "[Comparison of the radiosensitivity of chromosomes during the S and G2 periods in regenerating rat liver cells]. Radiosensitivity of chromosomes at S and G2 periods was studied by means of autoradiography technique in cells or regenerating liver of rats treated with X-rays at the doses of 150 and 300 r. The experiments were made so that the interval between irradiation and mitosis in S and G2 cells was equal. The results have shown that under the experimental conditions there are significant differences in the yield of chromosome aberrations between the labelled and intact cells. This withnesses for the existence of differences in radiosensitivity of chromosomes at different periods of mitotic cycle.", "PMID": 955412}
{"id": "pubmed23n0032_1651", "title": "The types of headache that affect the elderly.", "content": "Older people often describe their headaches as starting with vague neck discomfort and eventually moving to the temples and forehead. These are muscle-tension headaches, by far the most common type in the elderly. Although cervical osteoarthritis often is at fault, depression can be a significant factor, patricularly when headaches are chronic. There is no sure cure for tension headache, and often, several of the many remedies-ethyl chloride spray, moist heat, massage, antidepressant drugs, analgesics, local anesthetics, etc.-must be tried before an effective one is found. But just as important to successful therapy are concern, compassion, and a willingness to listen on the part of the physician. True migraine headaches are rare in the elderly. More prevalent is the type of vascular headache associated with giant cell arteritis, which is severe and resistant to any form of analgesic except the strongest narcotics. Vascular headaches also may result from congestive heart failure (which produces venous congestion in the cranial cavity), transient ischemia, increased intracranial pressure, and a variety of metabolic disturbances.", "contents": "The types of headache that affect the elderly. Older people often describe their headaches as starting with vague neck discomfort and eventually moving to the temples and forehead. These are muscle-tension headaches, by far the most common type in the elderly. Although cervical osteoarthritis often is at fault, depression can be a significant factor, patricularly when headaches are chronic. There is no sure cure for tension headache, and often, several of the many remedies-ethyl chloride spray, moist heat, massage, antidepressant drugs, analgesics, local anesthetics, etc.-must be tried before an effective one is found. But just as important to successful therapy are concern, compassion, and a willingness to listen on the part of the physician. True migraine headaches are rare in the elderly. More prevalent is the type of vascular headache associated with giant cell arteritis, which is severe and resistant to any form of analgesic except the strongest narcotics. Vascular headaches also may result from congestive heart failure (which produces venous congestion in the cranial cavity), transient ischemia, increased intracranial pressure, and a variety of metabolic disturbances.", "PMID": 955413}
{"id": "pubmed23n0032_1652", "title": "Conservative management of chronic renal failure.", "content": "Failing kidneys can play havoc with other parts of the body. Specific treatment of these associated problems may help ward off uremia and preserve whatever renal function remains. Sodium levels may drop if too much water is mistakenly given to counteract kidney failure. Hyperkalemia can lead to cardiac arrest if potassium levels aren't reduced without delay. Acidosis also may reach life-threatening proportions, especially if diarrhea occurs. Almost all patients with chronic renal failure have a bleeding tendency and anemia, with the hematocrit dipping as low as 20 percent. Over half have decreased tolerance to carbohydrares, although severe hyperglycemia is rare. Disorders of calcium metabolism also are common, ranging from asymptomatic hypocalcemia to osteomalacia. The kidneys' impaired filtration ability should be kept in mind when drugs are prescribed. Dosages may need to be cut to avoid an adverse reaction.", "contents": "Conservative management of chronic renal failure. Failing kidneys can play havoc with other parts of the body. Specific treatment of these associated problems may help ward off uremia and preserve whatever renal function remains. Sodium levels may drop if too much water is mistakenly given to counteract kidney failure. Hyperkalemia can lead to cardiac arrest if potassium levels aren't reduced without delay. Acidosis also may reach life-threatening proportions, especially if diarrhea occurs. Almost all patients with chronic renal failure have a bleeding tendency and anemia, with the hematocrit dipping as low as 20 percent. Over half have decreased tolerance to carbohydrares, although severe hyperglycemia is rare. Disorders of calcium metabolism also are common, ranging from asymptomatic hypocalcemia to osteomalacia. The kidneys' impaired filtration ability should be kept in mind when drugs are prescribed. Dosages may need to be cut to avoid an adverse reaction.", "PMID": 955417}
{"id": "pubmed23n0032_1653", "title": "Outpatient management of chronic bronchitis and emphysema.", "content": "Proper treatment relieves the symptoms of chronic bronchitis and emphysema to a large extent and helps patients remain active and useful until the last stages of the disease. Not much can be done to halt the natural course, since the disease is well advanced by the time that symptoms appear. Respiratory infections pose a constant threat. They are the primary cause of increased morbidity and mortality in these patients and may well accelerate the disease process. Annual prophylaxis against influenza is recommended, and antibiotic suppression should be considered for patients who have repeated bouts of acute bronchitis. Airways obstruction in emphysema is irreversible, but oral bronchodilators may remedy bronchospasm in chronic bronchitis and in emphysema with a bronchitic component. If sputum is thick and tenacious, postural drainage and chest physiotherapy may be helpful. Corticosteroids should be used only as a last resort. Patients tend to become inactive as the disease progresses. Exercise is important to increase exercise tolerance and overall physical fitness, and the physician should prescribe a specific daily program within the patient's limitations.", "contents": "Outpatient management of chronic bronchitis and emphysema. Proper treatment relieves the symptoms of chronic bronchitis and emphysema to a large extent and helps patients remain active and useful until the last stages of the disease. Not much can be done to halt the natural course, since the disease is well advanced by the time that symptoms appear. Respiratory infections pose a constant threat. They are the primary cause of increased morbidity and mortality in these patients and may well accelerate the disease process. Annual prophylaxis against influenza is recommended, and antibiotic suppression should be considered for patients who have repeated bouts of acute bronchitis. Airways obstruction in emphysema is irreversible, but oral bronchodilators may remedy bronchospasm in chronic bronchitis and in emphysema with a bronchitic component. If sputum is thick and tenacious, postural drainage and chest physiotherapy may be helpful. Corticosteroids should be used only as a last resort. Patients tend to become inactive as the disease progresses. Exercise is important to increase exercise tolerance and overall physical fitness, and the physician should prescribe a specific daily program within the patient's limitations.", "PMID": 955418}
{"id": "pubmed23n0032_1654", "title": "Gastrointestinal carcinoma: diagnosis, staging, and follow-up.", "content": "When cancer of the gastrointestinal tract is suspected, thorough studies should be made to determine the presence, type, and severity of the primary tumor and whether it has spread. Besides routine x-ray studies, angiography, scanning, and sonography may furnish valuable information. Endoscopy, which has revolutionized diagnosis of these tumors, not only makes it possible to obtain a specimen for histologic analysis without laparotomy but also reveals gross involvement as a guide to surgery. Staging of the lesion helps the physician select which patients would benefit from surgery and which instead should be treated with palliative chemotherapy or irradiation, or both. The patient's quality of life during treatment also must be considered. The side effects of multiple-agent chemotherapy, for example, may take too great a toll on the patient's ability to function. Continuous, long-term follow-up is essential. Metastases may develop after three to five years in particularly indolent tumors, and new primary lesions are far from rare.", "contents": "Gastrointestinal carcinoma: diagnosis, staging, and follow-up. When cancer of the gastrointestinal tract is suspected, thorough studies should be made to determine the presence, type, and severity of the primary tumor and whether it has spread. Besides routine x-ray studies, angiography, scanning, and sonography may furnish valuable information. Endoscopy, which has revolutionized diagnosis of these tumors, not only makes it possible to obtain a specimen for histologic analysis without laparotomy but also reveals gross involvement as a guide to surgery. Staging of the lesion helps the physician select which patients would benefit from surgery and which instead should be treated with palliative chemotherapy or irradiation, or both. The patient's quality of life during treatment also must be considered. The side effects of multiple-agent chemotherapy, for example, may take too great a toll on the patient's ability to function. Continuous, long-term follow-up is essential. Metastases may develop after three to five years in particularly indolent tumors, and new primary lesions are far from rare.", "PMID": 955419}
{"id": "pubmed23n0032_1655", "title": "Factors influencing food choices and intake.", "content": "To find out what elderly people eat and why, researchers interviewed 303 noninstitutionalized persons over 65. In addition to questions about the kinds and amounts of foods eaten, they quizzed the subjects on their eating patterns and their attitudes and knowledge about food and nutrition. The intakes of eight essential nutrients, calculated and compared with the Recommended Daily Dietary Allowances, were high overall. Some subjects, however, thought they ate better than they did. Eighty-three percent rated their own intake as good or excellent, but only about 60 percent actually fit this category. Diet did not correlate significantly with income, education, age, religion, or culture-probably due in part to the homogeneity of the sample-but some trends emerged. Even though the ratio of women to men was 3:1, the percentage of men who ate an excellent diet was higher than that of women in all four age groups. Also, each successive age group had a higher percentage of good or excellent scores.", "contents": "Factors influencing food choices and intake. To find out what elderly people eat and why, researchers interviewed 303 noninstitutionalized persons over 65. In addition to questions about the kinds and amounts of foods eaten, they quizzed the subjects on their eating patterns and their attitudes and knowledge about food and nutrition. The intakes of eight essential nutrients, calculated and compared with the Recommended Daily Dietary Allowances, were high overall. Some subjects, however, thought they ate better than they did. Eighty-three percent rated their own intake as good or excellent, but only about 60 percent actually fit this category. Diet did not correlate significantly with income, education, age, religion, or culture-probably due in part to the homogeneity of the sample-but some trends emerged. Even though the ratio of women to men was 3:1, the percentage of men who ate an excellent diet was higher than that of women in all four age groups. Also, each successive age group had a higher percentage of good or excellent scores.", "PMID": 955420}
{"id": "pubmed23n0032_1656", "title": "Recognizing spontaneous spinal epidural hematoma.", "content": "Sudden, often severe pain in the spinal region that is followed-sometimes within minutes-by paraplegia may signal spontaneous spinal epidural hematoma. This is a true surgical emergency calling for immediate laminectomy. Otherwise, the neurologic deficit becomes irreversible and the patient probably will die. The cause may be a fall or straining but in most cases there's no history of trauma. A myelogram reveals a block at the level of the hematoma, usually in the thoracic region or in the cervicothoracic or thoracolumbar area. Manometric tests shouldn't be done because they can increase the neurologic deficit. Urinary retention and sensitivity to pinprick at the level of the hematoma are additional diagnostic clues. The case reported here and 13 others from the literature point up the importance of prompt surgical treatment. Eight of the 12 patients who had laminectomy improved and five of them recovered completly, but both of the patients who were not treated surgically died.", "contents": "Recognizing spontaneous spinal epidural hematoma. Sudden, often severe pain in the spinal region that is followed-sometimes within minutes-by paraplegia may signal spontaneous spinal epidural hematoma. This is a true surgical emergency calling for immediate laminectomy. Otherwise, the neurologic deficit becomes irreversible and the patient probably will die. The cause may be a fall or straining but in most cases there's no history of trauma. A myelogram reveals a block at the level of the hematoma, usually in the thoracic region or in the cervicothoracic or thoracolumbar area. Manometric tests shouldn't be done because they can increase the neurologic deficit. Urinary retention and sensitivity to pinprick at the level of the hematoma are additional diagnostic clues. The case reported here and 13 others from the literature point up the importance of prompt surgical treatment. Eight of the 12 patients who had laminectomy improved and five of them recovered completly, but both of the patients who were not treated surgically died.", "PMID": 955421}
{"id": "pubmed23n0032_1657", "title": "Lysosomal acid hydrolase activities in the lungs of fetal, neonatal, adult, and senile mice.", "content": "The activities of four lysosomal acid hydrolases (LAH) in the lungs of two strains of mice changed significantly throughout the life cycle. In the CK7B1/6J animals, acid phosphatase (AP) and beta-glucuronidase (beta-G) were maximally active during early neonatal life then gradually declined the adult levels by 4-5 weeks of age. After reaching the adult level, acid phosphatase activities did not change significantly tcreased markedly with advanced age. N-Acetyl-beta-D-glucosaminidase (GAD) activities did not change significantly duringlate fetal, neonatal or young adult stages but increased significantly with advancing age. In the lungs of the CFW animals, the increase in activities of beta-G and GAD between young adult life and advanced age was highly significant, whereas there was no notable change in the activities of acid phosphatase or arylsulfatase (AS). The specific activities of the hydrolases in the lungs of the C57B1/6J strain were quite similar to those in the lungs of the CFW strain. The activities of all four hydrolases were markedly elevated in two spontaneous adenomatous tumors found in the lungs of old mice. The data indicate that LAH play a significant role in lung growth and maturation, and in changes associated with aging.", "contents": "Lysosomal acid hydrolase activities in the lungs of fetal, neonatal, adult, and senile mice. The activities of four lysosomal acid hydrolases (LAH) in the lungs of two strains of mice changed significantly throughout the life cycle. In the CK7B1/6J animals, acid phosphatase (AP) and beta-glucuronidase (beta-G) were maximally active during early neonatal life then gradually declined the adult levels by 4-5 weeks of age. After reaching the adult level, acid phosphatase activities did not change significantly tcreased markedly with advanced age. N-Acetyl-beta-D-glucosaminidase (GAD) activities did not change significantly duringlate fetal, neonatal or young adult stages but increased significantly with advancing age. In the lungs of the CFW animals, the increase in activities of beta-G and GAD between young adult life and advanced age was highly significant, whereas there was no notable change in the activities of acid phosphatase or arylsulfatase (AS). The specific activities of the hydrolases in the lungs of the C57B1/6J strain were quite similar to those in the lungs of the CFW strain. The activities of all four hydrolases were markedly elevated in two spontaneous adenomatous tumors found in the lungs of old mice. The data indicate that LAH play a significant role in lung growth and maturation, and in changes associated with aging.", "PMID": 955422}
{"id": "pubmed23n0032_1658", "title": "Concerning the estimation of biological age.", "content": "The conditions which must be satisfied by an index of biological age are discussed, and a high correlation with chronological age is shown to be neither a necessary nor a sufficient condition to be satisfied by any such index.", "contents": "Concerning the estimation of biological age. The conditions which must be satisfied by an index of biological age are discussed, and a high correlation with chronological age is shown to be neither a necessary nor a sufficient condition to be satisfied by any such index.", "PMID": 955423}
{"id": "pubmed23n0032_1659", "title": "Aging and accuracy of protein synthesis in man: search for inactive enzymatic cross-reacting material in granulocytes of aged people.", "content": "Enzyme activities and immunological reactivities of seven different enzymes have been assayed in the polymorphonuclear leukocytes from 10 newborns, 10 aged (over 80 years old) and 10 young adults (20-30 years old). Polymorphonuclears have been chosen because both in the newborns, and in the aged people, they are young cells whose life span is a few hours only. Five cytoplasmic enzymes have been immunologically studied by means of monospecific antienzyme sera: leukocyte pyruvate kinase by immunoinactivation, glucose-6-phosphate dehydrogenase and glucose phosphate-isomerase by electroimmunodiffusion, lactic dehydrogenase (muscle-type) by radial immunodiffusion. Two lysosomal enzymes (alpha-mannosidase and beta-glycuronidase) have been titrated immunologically with the same polyvalent antihuman leukocyte rabbit serum. Small but significant differences in activity were observed for several of the enzymes. By contrast, no significant difference was found, for any of the seven enzymes, as regards the ratio enzyme activity/immunological reactivity, between newborns, young adults and aged people. These results of not support the theory that a decreased accuracy of the protein synthesis machinery is responsible for aging and death. By contrast, the authors emphasize that the enzyme abnormalities reported by various authors in old animals or in old fibroblast cultures closely resemble those demonstrated to be \"post-translational modifications\" in the case of human glucose-6-phosphate dehydrogenase.", "contents": "Aging and accuracy of protein synthesis in man: search for inactive enzymatic cross-reacting material in granulocytes of aged people. Enzyme activities and immunological reactivities of seven different enzymes have been assayed in the polymorphonuclear leukocytes from 10 newborns, 10 aged (over 80 years old) and 10 young adults (20-30 years old). Polymorphonuclears have been chosen because both in the newborns, and in the aged people, they are young cells whose life span is a few hours only. Five cytoplasmic enzymes have been immunologically studied by means of monospecific antienzyme sera: leukocyte pyruvate kinase by immunoinactivation, glucose-6-phosphate dehydrogenase and glucose phosphate-isomerase by electroimmunodiffusion, lactic dehydrogenase (muscle-type) by radial immunodiffusion. Two lysosomal enzymes (alpha-mannosidase and beta-glycuronidase) have been titrated immunologically with the same polyvalent antihuman leukocyte rabbit serum. Small but significant differences in activity were observed for several of the enzymes. By contrast, no significant difference was found, for any of the seven enzymes, as regards the ratio enzyme activity/immunological reactivity, between newborns, young adults and aged people. These results of not support the theory that a decreased accuracy of the protein synthesis machinery is responsible for aging and death. By contrast, the authors emphasize that the enzyme abnormalities reported by various authors in old animals or in old fibroblast cultures closely resemble those demonstrated to be \"post-translational modifications\" in the case of human glucose-6-phosphate dehydrogenase.", "PMID": 955424}
{"id": "pubmed23n0032_1660", "title": "[Swelling of collagen fibers in neutral salt solutions (author's transl)].", "content": "When applying various concentrations of NaC104 (0.15-7 M) to tendon fibres from rat tails, we found denaturation beginning at 2M, and with increasing concentration a linear increase of the maximal contraction tension and an expoential shortening of the contraction time down to a minimal value of 7.5 min. In a second group of experiments we investigated the weight changes of tendons of old (26-30 months) and young (8 months) rats treated with 5M NaC104 and compared the weight curves and the tension curves. We found an increase in weight up to 900% as the tension decreased. This indicates a connection between the swelling and the relaxation of collagen fibres. Other solutions of neutral salts (5M NaC103, NaC1; 0.15 M NaC104, NaC103, NaC1) tested on young and old tendons did not produce any tension nor other visible changes. The weight curves show an immediate increase up to about 200% which remains constant and which is low compaired with the effect of 5M NaC104. The reason for the contraction of tendon fivres seems to be the denaturation caused by the increased lipophilia of the aqueous salt solutions, while the relaxation is merely the result of the subsequent swelling of the collagen fibre.", "contents": "[Swelling of collagen fibers in neutral salt solutions (author's transl)]. When applying various concentrations of NaC104 (0.15-7 M) to tendon fibres from rat tails, we found denaturation beginning at 2M, and with increasing concentration a linear increase of the maximal contraction tension and an expoential shortening of the contraction time down to a minimal value of 7.5 min. In a second group of experiments we investigated the weight changes of tendons of old (26-30 months) and young (8 months) rats treated with 5M NaC104 and compared the weight curves and the tension curves. We found an increase in weight up to 900% as the tension decreased. This indicates a connection between the swelling and the relaxation of collagen fibres. Other solutions of neutral salts (5M NaC103, NaC1; 0.15 M NaC104, NaC103, NaC1) tested on young and old tendons did not produce any tension nor other visible changes. The weight curves show an immediate increase up to about 200% which remains constant and which is low compaired with the effect of 5M NaC104. The reason for the contraction of tendon fivres seems to be the denaturation caused by the increased lipophilia of the aqueous salt solutions, while the relaxation is merely the result of the subsequent swelling of the collagen fibre.", "PMID": 955425}
{"id": "pubmed23n0032_1661", "title": "Lithocholate metabolism during chenotherapy for gallstone dissolution. 1. Serum levels of sulphated and unsulphated lithocholates.", "content": "Serum levels of total sulphated and total unsulphated lithocholates were each measured by a specific radioimmunoassay in 66 patients ingesting chenodeoxycholic (chenic) acid for gallstone dissoultion and in 35 gallstone patients ingesting either cholic acid or placebo. No changes occurred in serum lithocholate levels in the control groups. In patients ingesting chenic acid, there was a twofold increase in serum levels of total lithocholate, but the percent sulphation (greater than 75%) remained unchanged during chenotherapy. There was no correlation in the chenic acid treated group  between serum lithocholate levels and the proportion of lithocholate in biliary bile acids or changes in serum SGOT. The data suggest that there is effective sulphation of lithocholate in such patients; this may explain the lack of hepatotoxicity observed during ingestion of chenic acid.", "contents": "Lithocholate metabolism during chenotherapy for gallstone dissolution. 1. Serum levels of sulphated and unsulphated lithocholates. Serum levels of total sulphated and total unsulphated lithocholates were each measured by a specific radioimmunoassay in 66 patients ingesting chenodeoxycholic (chenic) acid for gallstone dissoultion and in 35 gallstone patients ingesting either cholic acid or placebo. No changes occurred in serum lithocholate levels in the control groups. In patients ingesting chenic acid, there was a twofold increase in serum levels of total lithocholate, but the percent sulphation (greater than 75%) remained unchanged during chenotherapy. There was no correlation in the chenic acid treated group  between serum lithocholate levels and the proportion of lithocholate in biliary bile acids or changes in serum SGOT. The data suggest that there is effective sulphation of lithocholate in such patients; this may explain the lack of hepatotoxicity observed during ingestion of chenic acid.", "PMID": 955495}
{"id": "pubmed23n0032_1662", "title": "Lithocholate metabolism during chemotherapy for gallstone dissolution. 2. Absorption and sulphation.", "content": "The amount of lithocholate input and the size of the total lithocholate exchangeable pool were measured using isotope dilution in three patients ingesting chenodeoxycholic acid for gallstone dissolution and two healthy control subjects.  Because the specific activity decay curve was biexponential in appearance, input was calculated  using a stochastic analysis employing the  Stewart-Hamilton equation. By this method, the lithocholate input and the size of the exchangeable pool in gallstone patients during chenic acid ingestion was four to five times that observed in the healthy control subjects. It was calculated that about one-fifth of the newly formed lithocholate was absorbed in both gallstone patients and healthy control subjects. The extent of sulphation of absorbed lithocholate was then measured in seven gallstone patients whto had been ingesting chenodeoxycholic acid for gallstone dissolution by defining the chemical form of radioactivity in bile after intravenous administration of labelled lithocholate. Similar studies were carried out in eight healthy control subjects, but, in addition the extent of sulphation of injected lithocholylglycine and lithocholyltaurine was also defined. When lithocholate was injected, the majority of radioactivity in bile (50-60%) was present as sulphated conjugates, both in gallstone patients and healthy control subjects. The degree of sulphation was greater for glycine than taurine conjugates, and these findings, which suggested preferential sulphation of the glycine conjugates of lithocholate, were confirmed by showing that injected lithocholylglycine was sulphated to a much greater extent than injected lithocholyltaurine. These findings indicate that in patients receiving chenic acid there is effective sulphation of lithocholate, especially its glycine conjugates, despite a considerable increase in lithocholate absorption; they may provide an explanation for the lack of hepatotoxicity of chenic acid in man.", "contents": "Lithocholate metabolism during chemotherapy for gallstone dissolution. 2. Absorption and sulphation. The amount of lithocholate input and the size of the total lithocholate exchangeable pool were measured using isotope dilution in three patients ingesting chenodeoxycholic acid for gallstone dissolution and two healthy control subjects.  Because the specific activity decay curve was biexponential in appearance, input was calculated  using a stochastic analysis employing the  Stewart-Hamilton equation. By this method, the lithocholate input and the size of the exchangeable pool in gallstone patients during chenic acid ingestion was four to five times that observed in the healthy control subjects. It was calculated that about one-fifth of the newly formed lithocholate was absorbed in both gallstone patients and healthy control subjects. The extent of sulphation of absorbed lithocholate was then measured in seven gallstone patients whto had been ingesting chenodeoxycholic acid for gallstone dissolution by defining the chemical form of radioactivity in bile after intravenous administration of labelled lithocholate. Similar studies were carried out in eight healthy control subjects, but, in addition the extent of sulphation of injected lithocholylglycine and lithocholyltaurine was also defined. When lithocholate was injected, the majority of radioactivity in bile (50-60%) was present as sulphated conjugates, both in gallstone patients and healthy control subjects. The degree of sulphation was greater for glycine than taurine conjugates, and these findings, which suggested preferential sulphation of the glycine conjugates of lithocholate, were confirmed by showing that injected lithocholylglycine was sulphated to a much greater extent than injected lithocholyltaurine. These findings indicate that in patients receiving chenic acid there is effective sulphation of lithocholate, especially its glycine conjugates, despite a considerable increase in lithocholate absorption; they may provide an explanation for the lack of hepatotoxicity of chenic acid in man.", "PMID": 955496}
{"id": "pubmed23n0032_1663", "title": "Plasma oestrogens in men with chronic liver disease.", "content": "A highly specific radioimmunoassay was used to measure the total plasma concentrations of the three principal unconjugated oestrogens: oestrone E1, oestradiol E2, and oestriol E3 in normal males and in 21 males with various forms of chronic liver disease. In addition, the unbound concentration of plasma E2 was established in the same group. About half of the patients with liver disease had overt feminising changes. Total and unbound plasma E2 concentrations were within the normal range in all patients. Total plasma E1 was significantly elevated only in those patients with liver disease and gynaecomastia, and a similar trend was seen for total plasma E3.", "contents": "Plasma oestrogens in men with chronic liver disease. A highly specific radioimmunoassay was used to measure the total plasma concentrations of the three principal unconjugated oestrogens: oestrone E1, oestradiol E2, and oestriol E3 in normal males and in 21 males with various forms of chronic liver disease. In addition, the unbound concentration of plasma E2 was established in the same group. About half of the patients with liver disease had overt feminising changes. Total and unbound plasma E2 concentrations were within the normal range in all patients. Total plasma E1 was significantly elevated only in those patients with liver disease and gynaecomastia, and a similar trend was seen for total plasma E3.", "PMID": 955497}
{"id": "pubmed23n0032_1664", "title": "Use of caerulein with submaximal doses of secretin as a test of pancreatic function in man.", "content": "The pancreatic exocrine secretory response to different combinations of the pancreozymin-like peptide caerulein and secretin was studied in 110 healthy control subjects and 32 patients with pancreatic disease. A combination of 0-5 CU/kg-h secretin and 75 ng/kg-h caerulein provided maximal or near-maximal stimulation of the secretion of both bicarbonate and pancreatic enzymes. The combination of stimulant peptides in the above doses was found to give excellent separation between the secretion of bicarbonate, lipase, and chymotrypsin of the control group and patients with chronic pancreatitis. A combination of caerulein and secretin represents a safe, reliable, and effective mean of stimulating pancreatic exocrine secretion for the purpose of testing pancreatic function.", "contents": "Use of caerulein with submaximal doses of secretin as a test of pancreatic function in man. The pancreatic exocrine secretory response to different combinations of the pancreozymin-like peptide caerulein and secretin was studied in 110 healthy control subjects and 32 patients with pancreatic disease. A combination of 0-5 CU/kg-h secretin and 75 ng/kg-h caerulein provided maximal or near-maximal stimulation of the secretion of both bicarbonate and pancreatic enzymes. The combination of stimulant peptides in the above doses was found to give excellent separation between the secretion of bicarbonate, lipase, and chymotrypsin of the control group and patients with chronic pancreatitis. A combination of caerulein and secretin represents a safe, reliable, and effective mean of stimulating pancreatic exocrine secretion for the purpose of testing pancreatic function.", "PMID": 955498}
{"id": "pubmed23n0032_1665", "title": "Effect of intraduodenal magnesium sulphate on pancreas and gallbladder of man.", "content": "Magnesium sulphate has previously been used as a purgative in a test involving the measurement of the faecal excretion of pancreatic enzymes. In order to validate the use of magnesium sulphate for this purpose, in 18 individuals the pancreatic and biliary response to intravenous infusion of secretin (1 CU/kg-h) plus CCK(1IU/kg-h) were compared with the responses to one of three dose-rates of magnesium sulphate infused into the duodenum. The effect of magnesium sulhphate was  also studied during the coincident intravenous administration of the hormones. Intraduodenal magnesium sulphate did not stimulate the secretion of bicarbonate into the duodenum but did  evoke the secretion of pancreatic enzymes and discharge of bile. The pancreatic response to the exogenous hormones was not altered by coincident intraduodenal infusion of magnesium sulphate. We conclude that magnesium sulphate is a satisfactory purgative for speeding the intestinal transit of pancreatic enzymes.", "contents": "Effect of intraduodenal magnesium sulphate on pancreas and gallbladder of man. Magnesium sulphate has previously been used as a purgative in a test involving the measurement of the faecal excretion of pancreatic enzymes. In order to validate the use of magnesium sulphate for this purpose, in 18 individuals the pancreatic and biliary response to intravenous infusion of secretin (1 CU/kg-h) plus CCK(1IU/kg-h) were compared with the responses to one of three dose-rates of magnesium sulphate infused into the duodenum. The effect of magnesium sulhphate was  also studied during the coincident intravenous administration of the hormones. Intraduodenal magnesium sulphate did not stimulate the secretion of bicarbonate into the duodenum but did  evoke the secretion of pancreatic enzymes and discharge of bile. The pancreatic response to the exogenous hormones was not altered by coincident intraduodenal infusion of magnesium sulphate. We conclude that magnesium sulphate is a satisfactory purgative for speeding the intestinal transit of pancreatic enzymes.", "PMID": 955499}
{"id": "pubmed23n0032_1666", "title": "Radiographic evaluation of pancreatico-jejunal shunts.", "content": "This paper describes opacification and identification of two surgically constructed pancreatico-jujunal shunts. A fibreoptic panendoscope was used with retrograde injection via the ampulla of  Vater (ERCP). This procedure makes possible more accurate anatomical evaluation and so more precise clinical appraisal of both pre- and post-surgical states.", "contents": "Radiographic evaluation of pancreatico-jejunal shunts. This paper describes opacification and identification of two surgically constructed pancreatico-jujunal shunts. A fibreoptic panendoscope was used with retrograde injection via the ampulla of  Vater (ERCP). This procedure makes possible more accurate anatomical evaluation and so more precise clinical appraisal of both pre- and post-surgical states.", "PMID": 955500}
{"id": "pubmed23n0032_1667", "title": "Electrogenic glucose absorption in untreated and treated coeliac disease.", "content": "Using a method for measuring changes in transmural potential difference across the human jejunum in vivo, the operational kinetic parameters of 'Apparent Km' and PD max for the active electrogenic component of glucose absorption were estimated in a group of healthy volunteers and in patients with coeliac disease. Both the 'Apparent Km' (17+/2mM; mean +/SEM) and the PD max (8.6+/0.7 mV) in nine patients with untreated coeliac disease were significantly lower (p less than 0.005) than in the control group ('Apparent Km' = 74+/5mM; PD max 12.8+/0.9mV, n=20). Treatment of five coeliac patients by gluten withdrawal for less than three months  increased significantly the values of both the \"Apparent Km (35+/6mM) and the TPD max (11.4+/1.2mV). Treatment of five patients for more than six months caused a further increase in the values of both kinetic parameters ('Apparent Km' = 108+/13mM; PD max  =15.6+/2.7mV) to levels which exceeded those in healthy subjects. The possible interpretations  of the differences in the kinetic characteristics of electrogenic glucose transport between coeliac patients and healthy subjects are discussed.", "contents": "Electrogenic glucose absorption in untreated and treated coeliac disease. Using a method for measuring changes in transmural potential difference across the human jejunum in vivo, the operational kinetic parameters of 'Apparent Km' and PD max for the active electrogenic component of glucose absorption were estimated in a group of healthy volunteers and in patients with coeliac disease. Both the 'Apparent Km' (17+/2mM; mean +/SEM) and the PD max (8.6+/0.7 mV) in nine patients with untreated coeliac disease were significantly lower (p less than 0.005) than in the control group ('Apparent Km' = 74+/5mM; PD max 12.8+/0.9mV, n=20). Treatment of five coeliac patients by gluten withdrawal for less than three months  increased significantly the values of both the \"Apparent Km (35+/6mM) and the TPD max (11.4+/1.2mV). Treatment of five patients for more than six months caused a further increase in the values of both kinetic parameters ('Apparent Km' = 108+/13mM; PD max  =15.6+/2.7mV) to levels which exceeded those in healthy subjects. The possible interpretations  of the differences in the kinetic characteristics of electrogenic glucose transport between coeliac patients and healthy subjects are discussed.", "PMID": 955501}
{"id": "pubmed23n0032_1668", "title": "Factors affecting the absorption of vitamin K-1 in vitro.", "content": "Factors which might affect the absorption of vitamin K of dietary origin were investigated using everted small bowel sacs. Increasing the bile salt concentration to 20 mM or the addition of long chain fatty acids, monoolein, or lecithin all resulted in significant (P less than 0-05) decrease in the absorption rate of the vitamin. The addition of 2-5 mM short and medium chain fatty acids did not change the absorption rate of vitamin K-1 (P greater than 0-05). The absorption rate of vitamin K-1 appears to be modified by the presence of compounds in the incubation medium which either alter the partition of the vitamin between the micelle and the cell membrane or which change the permeation characteristics of the compound through the unstirred water layer or modify the physical characteristics of the cell membrane itself. It is possible that some of the above factors modify the absorption of lipid soluble compounds in general.", "contents": "Factors affecting the absorption of vitamin K-1 in vitro. Factors which might affect the absorption of vitamin K of dietary origin were investigated using everted small bowel sacs. Increasing the bile salt concentration to 20 mM or the addition of long chain fatty acids, monoolein, or lecithin all resulted in significant (P less than 0-05) decrease in the absorption rate of the vitamin. The addition of 2-5 mM short and medium chain fatty acids did not change the absorption rate of vitamin K-1 (P greater than 0-05). The absorption rate of vitamin K-1 appears to be modified by the presence of compounds in the incubation medium which either alter the partition of the vitamin between the micelle and the cell membrane or which change the permeation characteristics of the compound through the unstirred water layer or modify the physical characteristics of the cell membrane itself. It is possible that some of the above factors modify the absorption of lipid soluble compounds in general.", "PMID": 955502}
{"id": "pubmed23n0032_1669", "title": "Relation between body weight and the gastric and intestinal handling of an oral caloric load.", "content": "The gastric and intestinal handling of a standard liquid 300-ml meal (0.89 kcal/ml) was examined in 10 healthy, non-obese subjects by a quantitative multiple-indicator dilution technique. Such a meal contains about one-tenth of the daily energy requirements. The heavier the subject the more energy was transferred from the stomach to the intestine during the first 80 minutes after taking the meal. A high body weight was also associated with a rapid transit through the proximal 70 cm of intestine and the passing of a substantial part of the meal calories to the lower intestine. These circumstances favour a fast incorporation of fed calories. In subjects with lower body weights eneryg  was delivered more slowly from the stomach. The absorbed an equal amount of energy in the proximal intestine as did the heavier subjects, but during the longer transit times. The percentage absorption in the investigated segment was therefore higher.", "contents": "Relation between body weight and the gastric and intestinal handling of an oral caloric load. The gastric and intestinal handling of a standard liquid 300-ml meal (0.89 kcal/ml) was examined in 10 healthy, non-obese subjects by a quantitative multiple-indicator dilution technique. Such a meal contains about one-tenth of the daily energy requirements. The heavier the subject the more energy was transferred from the stomach to the intestine during the first 80 minutes after taking the meal. A high body weight was also associated with a rapid transit through the proximal 70 cm of intestine and the passing of a substantial part of the meal calories to the lower intestine. These circumstances favour a fast incorporation of fed calories. In subjects with lower body weights eneryg  was delivered more slowly from the stomach. The absorbed an equal amount of energy in the proximal intestine as did the heavier subjects, but during the longer transit times. The percentage absorption in the investigated segment was therefore higher.", "PMID": 955503}
{"id": "pubmed23n0032_1670", "title": "Gastric mucosa after partial gastrectomy.", "content": "A partial gastrectomy of Billroth I  or II type was performed in a series of 146 patients with peptic ulcer. Gastric biopsy was carried out two years later and the histology of the specimens compared with that of the body mucosa at the time of operation. In 138 patients without body atrophic  gastritis (AG) before operation this condition was found in 74 (54%) two years after (46% of DU patients and 73% of GU patients). Those with antral or pyloric  canal ulcers were particularly liable to develop AG (81%). Apart from site of ulcer various other factors possibly associated with the development of AG were examined: no positive correlations were found with the possible exception of anaemia. Gastric parietal cell antibodies were not found in any patient with AG tested. The cause of gastritis after partial gastrectomy and its possible relationship with gastric carcinoma are discussed.", "contents": "Gastric mucosa after partial gastrectomy. A partial gastrectomy of Billroth I  or II type was performed in a series of 146 patients with peptic ulcer. Gastric biopsy was carried out two years later and the histology of the specimens compared with that of the body mucosa at the time of operation. In 138 patients without body atrophic  gastritis (AG) before operation this condition was found in 74 (54%) two years after (46% of DU patients and 73% of GU patients). Those with antral or pyloric  canal ulcers were particularly liable to develop AG (81%). Apart from site of ulcer various other factors possibly associated with the development of AG were examined: no positive correlations were found with the possible exception of anaemia. Gastric parietal cell antibodies were not found in any patient with AG tested. The cause of gastritis after partial gastrectomy and its possible relationship with gastric carcinoma are discussed.", "PMID": 955504}
{"id": "pubmed23n0032_1671", "title": "Highly selective vagotomy plus dilatation of the stenosis compared with truncal vagotomy and drainage in the treatment of pyloric stenosis secondary to duodenal ulceration.", "content": "Twenty-three consecutive patients with duodenal ulceration complicated by pyloric stenosis who came under the care of one surgeon were treated by highly selective vagotomy (HSV) combined with digital dilatation of the stenosis through  a gastrotomy. No form of drainage procedure was used. Thus the antral \"mill\" and the pyloric sphineter were left intact. Since the stenosis is usually distal to the pylorus rather than truly pyloric such dilatation does not damage the pyloric ring, although it may on occasion lead to perforation of the first part of the duodenum. The subsequent progress of these patients was compared with that of a similar, consecutive series of 23 patients with pyloric stenosis who were treated by truncal vagotomy with a drainage procedure by other surgeons on the same surgical unit. Patients were followed up for between four months and five years. The clinical assessment was carried out in \"blind\" fashion at a special gastric follow-up clinic. No evidence of recurrent ulceration was found in either group of patients. Two patients from each group subsequently  came to reoperation for the relief of gastric stasis. Twenty-two of the 23 patients (96%) who had undergone HSV plus dilatation eventually achieved a good-to-excellent clinical result (Visick grades 1+2), wheras only 17 of the 23 patients (74%) who had undergone truncal vagotomy with drainage achieved such a result. The main clinical difference between the two groups was that side effects such as diarrhoea and abdominal pain or discomfort were more common after vagotomy with drainage than after HSV. These results bear witness to the remarkable propulsive powers of the gastric antrum after HSV,  which were evidently sufficient to overcome any tendency to re-stenosis in more than 90% of patients. The 9% incidence of failure due to re-stenosis could perhaps be avoided if a small duodenoplasty were performed instead of simple digital dilatation. The results support the hypothesis that damage to the antral mill and pyloric sphincter can be avoided in the course of operations for \"pyloric\" stenosis secondary to duodenal ulceration. Avoidance of the drainage procedure is of benefit to the patient, just  as it is in patients who have duodenal ulceration without stenosis.", "contents": "Highly selective vagotomy plus dilatation of the stenosis compared with truncal vagotomy and drainage in the treatment of pyloric stenosis secondary to duodenal ulceration. Twenty-three consecutive patients with duodenal ulceration complicated by pyloric stenosis who came under the care of one surgeon were treated by highly selective vagotomy (HSV) combined with digital dilatation of the stenosis through  a gastrotomy. No form of drainage procedure was used. Thus the antral \"mill\" and the pyloric sphineter were left intact. Since the stenosis is usually distal to the pylorus rather than truly pyloric such dilatation does not damage the pyloric ring, although it may on occasion lead to perforation of the first part of the duodenum. The subsequent progress of these patients was compared with that of a similar, consecutive series of 23 patients with pyloric stenosis who were treated by truncal vagotomy with a drainage procedure by other surgeons on the same surgical unit. Patients were followed up for between four months and five years. The clinical assessment was carried out in \"blind\" fashion at a special gastric follow-up clinic. No evidence of recurrent ulceration was found in either group of patients. Two patients from each group subsequently  came to reoperation for the relief of gastric stasis. Twenty-two of the 23 patients (96%) who had undergone HSV plus dilatation eventually achieved a good-to-excellent clinical result (Visick grades 1+2), wheras only 17 of the 23 patients (74%) who had undergone truncal vagotomy with drainage achieved such a result. The main clinical difference between the two groups was that side effects such as diarrhoea and abdominal pain or discomfort were more common after vagotomy with drainage than after HSV. These results bear witness to the remarkable propulsive powers of the gastric antrum after HSV,  which were evidently sufficient to overcome any tendency to re-stenosis in more than 90% of patients. The 9% incidence of failure due to re-stenosis could perhaps be avoided if a small duodenoplasty were performed instead of simple digital dilatation. The results support the hypothesis that damage to the antral mill and pyloric sphincter can be avoided in the course of operations for \"pyloric\" stenosis secondary to duodenal ulceration. Avoidance of the drainage procedure is of benefit to the patient, just  as it is in patients who have duodenal ulceration without stenosis.", "PMID": 955505}
{"id": "pubmed23n0032_1672", "title": "Proctocolitis and Crohn's disease of the colon: a comparison of the clinical course.", "content": "This study suggests that proctocolitis is an illness with an acute and potentially dangerous onset but which appears to become less severe after survival for one year, whereas Crohn's colitis tends to be a more chronic and progressive illness over several years with a greater need for surgical treatment and a greater tendency to anal complications.", "contents": "Proctocolitis and Crohn's disease of the colon: a comparison of the clinical course. This study suggests that proctocolitis is an illness with an acute and potentially dangerous onset but which appears to become less severe after survival for one year, whereas Crohn's colitis tends to be a more chronic and progressive illness over several years with a greater need for surgical treatment and a greater tendency to anal complications.", "PMID": 955506}
{"id": "pubmed23n0032_1673", "title": "Fibrin thrombi, a cause of clindamycin-associated colitis?", "content": "Rectal biopsies from five patients with clindamycin-associated colitis were studied with the intention of identifying fibrin thrombi in capillaries. Capillary thrombosis was present in all five cases, three with pseudomembranous colitis and two with non-specific colitis. The significance of capillary thrombosis and its relationship to other ischaemic lesions of the gut are discussed.", "contents": "Fibrin thrombi, a cause of clindamycin-associated colitis? Rectal biopsies from five patients with clindamycin-associated colitis were studied with the intention of identifying fibrin thrombi in capillaries. Capillary thrombosis was present in all five cases, three with pseudomembranous colitis and two with non-specific colitis. The significance of capillary thrombosis and its relationship to other ischaemic lesions of the gut are discussed.", "PMID": 955507}
{"id": "pubmed23n0032_1674", "title": "New early tertiary primates and a reappraisal of some Plesiadapiformes.", "content": "A new study of specimens of paromomyid and microsyopid primates clarifies evolution in these primates and reuslts in new concepts of each family. The paromomyid Phenacolemur and its former synonym, Ignacius, are distinct genera, each with new Early Wasatchian species, Plesiolestes, Palaechthon, Torrejonia, Palenochtha, Navajovius, Micromomys, and Tinimomys are removed from the Paromomyidae and transferred to the Microsyopidae. The Plesiadapiformes are classified in three groups which may be differentiated on incisor and molor structure: Plesiadapoidea (Plesiadapidae, Carpolestidae, and Saxonellidae), Microsyopidae, and Paromomyidae. The Paromomyidae, Microsyopidae (and its two subfamilies), and several genera are rediagnosed in the light of new comparative evidence.", "contents": "New early tertiary primates and a reappraisal of some Plesiadapiformes. A new study of specimens of paromomyid and microsyopid primates clarifies evolution in these primates and reuslts in new concepts of each family. The paromomyid Phenacolemur and its former synonym, Ignacius, are distinct genera, each with new Early Wasatchian species, Plesiolestes, Palaechthon, Torrejonia, Palenochtha, Navajovius, Micromomys, and Tinimomys are removed from the Paromomyidae and transferred to the Microsyopidae. The Plesiadapiformes are classified in three groups which may be differentiated on incisor and molor structure: Plesiadapoidea (Plesiadapidae, Carpolestidae, and Saxonellidae), Microsyopidae, and Paromomyidae. The Paromomyidae, Microsyopidae (and its two subfamilies), and several genera are rediagnosed in the light of new comparative evidence.", "PMID": 955516}
{"id": "pubmed23n0032_1675", "title": "[Surgical use of the laser].", "content": "The suitability of the laser for use in surgery is discussed. The mode of action as well as the advantages and disadvantages of electro-cautery are described together with a discussion about the use of the laser as an alternative. After preliminary remarks about the physical aspects, the effects of laser rays on biological tissue are illustrated and the most important properties of the laser with regard to its use in surgery are pointed out. An outline is given of the present use of the laser in the experimental and clinical areas; the properties required of a cutting instrument in surgery are listed. Finally the influence of the rays on the cutting characteristics of a Nd-high-power-laser is investigated in experiments using a rat liver model. This laser has substantial advantages over the CO2-laser in its practical application. The cutting mechanism and the development of the necrotic areas are discussed, together with a description of the cessation of hemorrhage and wound healing. The results obtained indicate that as soon as technical problems can be solved, the use of a pulsed high power YAG laser as a cutting instrument in surgery is highly promising.", "contents": "[Surgical use of the laser]. The suitability of the laser for use in surgery is discussed. The mode of action as well as the advantages and disadvantages of electro-cautery are described together with a discussion about the use of the laser as an alternative. After preliminary remarks about the physical aspects, the effects of laser rays on biological tissue are illustrated and the most important properties of the laser with regard to its use in surgery are pointed out. An outline is given of the present use of the laser in the experimental and clinical areas; the properties required of a cutting instrument in surgery are listed. Finally the influence of the rays on the cutting characteristics of a Nd-high-power-laser is investigated in experiments using a rat liver model. This laser has substantial advantages over the CO2-laser in its practical application. The cutting mechanism and the development of the necrotic areas are discussed, together with a description of the cessation of hemorrhage and wound healing. The results obtained indicate that as soon as technical problems can be solved, the use of a pulsed high power YAG laser as a cutting instrument in surgery is highly promising.", "PMID": 955531}
{"id": "pubmed23n0032_1676", "title": "[Imprint cytology. Advantages and possibilities].", "content": "Imprint cytology is a special variation of applied cytology, which can be used for quite different purposes. In scientific research cytologic imprints are very apt for cytophotometric measurements of DNA contents in nuclei as well as for karyologic studies, for instance on the different distribution of chromatin in the nuclei of benign and malignant tumors. Furthermore, imprint cytology is of great importance in teaching and learning results of aspiration biopsies, as the cellular patterns obtained with both methods are looking identical and moreover every imprint can be compared with the corresponding histologic slide. Last but not least in diagnostic procedures cytologic imprints are very helpful as an adjuvant to frozen and paraffin sections: They set off also very discrete changes and make possible diagnostic statements in a shorter time than it is possible with histologic slides.", "contents": "[Imprint cytology. Advantages and possibilities]. Imprint cytology is a special variation of applied cytology, which can be used for quite different purposes. In scientific research cytologic imprints are very apt for cytophotometric measurements of DNA contents in nuclei as well as for karyologic studies, for instance on the different distribution of chromatin in the nuclei of benign and malignant tumors. Furthermore, imprint cytology is of great importance in teaching and learning results of aspiration biopsies, as the cellular patterns obtained with both methods are looking identical and moreover every imprint can be compared with the corresponding histologic slide. Last but not least in diagnostic procedures cytologic imprints are very helpful as an adjuvant to frozen and paraffin sections: They set off also very discrete changes and make possible diagnostic statements in a shorter time than it is possible with histologic slides.", "PMID": 955532}
{"id": "pubmed23n0032_1677", "title": "[Fibrinogen degradation products in gynecologic neoplasms].", "content": "Concentration of fibrinogen degradation products in sera of 12 healthy women and 163 patients with gynecological tumors was determined by Staphylococcal-clumping-test. In sera of healthy women and patients with benign tumors the highest titer was 8. On the other hand in 53 of 97 cases with malign tumors higher concentrations were evaluated.", "contents": "[Fibrinogen degradation products in gynecologic neoplasms]. Concentration of fibrinogen degradation products in sera of 12 healthy women and 163 patients with gynecological tumors was determined by Staphylococcal-clumping-test. In sera of healthy women and patients with benign tumors the highest titer was 8. On the other hand in 53 of 97 cases with malign tumors higher concentrations were evaluated.", "PMID": 955534}
{"id": "pubmed23n0032_1678", "title": "[Radiotherapy in the treatment of breast carcinoma].", "content": "In the treatment of breast cancer a radiation therapy is indicated under the following conditions: 1. Postoperative irradiation only of the regional lymph-nodes also in stage I (T1, N0). 2. Postoperative irradiation of the regional lymph-nodes and the thorax wall in cases with great primary tumours (T2), in cases with involved axillary lymph-nodes and, of course, in all cases with \"grave signs\". 3. Preoperative irradiation only in those cases when it seems possible that an inoperable tumour would become operable. 4. As the sole local treatment only in cases with very large inoperable tumours or in special cases (e.g. very high risc or refusal of the operation). 5. As local treatment of a local recidive or of isolated metastases. 6. As supporting local therapy (e.g. threatening fracturation of our fracturated bone metastases; brain metastases) in cases of generalized metastatic disease treated by hormonal or cytostatic therapy.", "contents": "[Radiotherapy in the treatment of breast carcinoma]. In the treatment of breast cancer a radiation therapy is indicated under the following conditions: 1. Postoperative irradiation only of the regional lymph-nodes also in stage I (T1, N0). 2. Postoperative irradiation of the regional lymph-nodes and the thorax wall in cases with great primary tumours (T2), in cases with involved axillary lymph-nodes and, of course, in all cases with \"grave signs\". 3. Preoperative irradiation only in those cases when it seems possible that an inoperable tumour would become operable. 4. As the sole local treatment only in cases with very large inoperable tumours or in special cases (e.g. very high risc or refusal of the operation). 5. As local treatment of a local recidive or of isolated metastases. 6. As supporting local therapy (e.g. threatening fracturation of our fracturated bone metastases; brain metastases) in cases of generalized metastatic disease treated by hormonal or cytostatic therapy.", "PMID": 955535}
{"id": "pubmed23n0032_1679", "title": "[Polychemotherapy of the advanced and metastasizing carcinoma of the breast].", "content": "The course of treatment with polychemotherapy for the progressed and metastasized breast-carcinoma is discussed. The therapy with cytostatics must be continued for the patients entire life. A sample of 27 patients from the Chir; Univ.-Klinik Innsbruck with a course of treatment lasting from 15 to 29 months (October 1973 resp; December 1974 to February 1976) showed a success rate of 81%.", "contents": "[Polychemotherapy of the advanced and metastasizing carcinoma of the breast]. The course of treatment with polychemotherapy for the progressed and metastasized breast-carcinoma is discussed. The therapy with cytostatics must be continued for the patients entire life. A sample of 27 patients from the Chir; Univ.-Klinik Innsbruck with a course of treatment lasting from 15 to 29 months (October 1973 resp; December 1974 to February 1976) showed a success rate of 81%.", "PMID": 955536}
{"id": "pubmed23n0032_1680", "title": "[Cancer therapy using intermittent high doses of cytostatics].", "content": "The results of intermittent high-dose-therapy, especially with Doxorubicin and Amethopterin, are encouraging, particularly, against those malignant tumors which were difficult to influence until now. The damage to normal tissue and especially the therapy-limiting cumulative toxicity, seem to be lower if compared with the continuous low-dose-treatment because of the possibility for complete recovery of normal tissue during therapy-free intervals. Correlations between tumor, normal tissue and cytotoxic agent are discussed on the basis of cellkinetic findings to contribute to a better understanding of the way of action of a high-dose-therapy.", "contents": "[Cancer therapy using intermittent high doses of cytostatics]. The results of intermittent high-dose-therapy, especially with Doxorubicin and Amethopterin, are encouraging, particularly, against those malignant tumors which were difficult to influence until now. The damage to normal tissue and especially the therapy-limiting cumulative toxicity, seem to be lower if compared with the continuous low-dose-treatment because of the possibility for complete recovery of normal tissue during therapy-free intervals. Correlations between tumor, normal tissue and cytotoxic agent are discussed on the basis of cellkinetic findings to contribute to a better understanding of the way of action of a high-dose-therapy.", "PMID": 955537}
{"id": "pubmed23n0032_1681", "title": "[Unusual angiographic examination methods in bronchial carcinoma. Attempt at critical evaluation].", "content": "The examination of mediastinal and pulmonary processes with angiographic methods play a decisive role in diagnosis. The results could be improved by combination of the procedures.", "contents": "[Unusual angiographic examination methods in bronchial carcinoma. Attempt at critical evaluation]. The examination of mediastinal and pulmonary processes with angiographic methods play a decisive role in diagnosis. The results could be improved by combination of the procedures.", "PMID": 955538}
{"id": "pubmed23n0032_1682", "title": "[Changes in the mouth mucosa during pregnancy and hormonal contraceptive treatment].", "content": "The oral mucous membrane, especially the gingiva, may undergo changes during pregnancy which, from clinical and histological observations, should be termed pregnancy gingival hyperplasia. Such rare cases of hyperplasia mimic pregnancy gingival tumours (granuloma gravidarum) within the buccal cavity. The gingival changes observed occasionally in women taking oral contraceptives may also be due to hormonal causes.", "contents": "[Changes in the mouth mucosa during pregnancy and hormonal contraceptive treatment]. The oral mucous membrane, especially the gingiva, may undergo changes during pregnancy which, from clinical and histological observations, should be termed pregnancy gingival hyperplasia. Such rare cases of hyperplasia mimic pregnancy gingival tumours (granuloma gravidarum) within the buccal cavity. The gingival changes observed occasionally in women taking oral contraceptives may also be due to hormonal causes.", "PMID": 955542}
{"id": "pubmed23n0032_1683", "title": "[Frequency of epithelial changes in the uterine cervix in adolescents and young women].", "content": "From 1967 to 1974 vaginal and cervical smears of about 46,000 teenage girls and young women up to the age of 25 were examined. Among these were 250 doubtful and positive cases (0,54%). Of the 67 positive ones 46 cases were known to be histologically examined (69%); 8 times an invasive carcinoma and 38 times a carcinoma in situ were found. Women under the age of 25 should therefore be included in routine check-up with cervical and vaginal smears.", "contents": "[Frequency of epithelial changes in the uterine cervix in adolescents and young women]. From 1967 to 1974 vaginal and cervical smears of about 46,000 teenage girls and young women up to the age of 25 were examined. Among these were 250 doubtful and positive cases (0,54%). Of the 67 positive ones 46 cases were known to be histologically examined (69%); 8 times an invasive carcinoma and 38 times a carcinoma in situ were found. Women under the age of 25 should therefore be included in routine check-up with cervical and vaginal smears.", "PMID": 955543}
{"id": "pubmed23n0032_1684", "title": "[Consultation for abortion. Experiences and results].", "content": "During the 12 month period June 1974-June 1975, 170 patients were advised on termination of pregnancy in the University Department of Obstetrics and Gynaecology, Mannheim, the results from 145 women are discussed. 74% of the women were married, the majority of whom were between 34-39 years old. 39% lived in bad, 40% in average and 21% in good social conditions. In 77 women the pregnancy was terminated after permission was granted from the health authority. In 43 patients the termination was not granted and 25 women did not return to the clinic when an expert opinion was demanded. In 50 women a definitive sterilisation was carried out at the same time as termination of the pregnancy.", "contents": "[Consultation for abortion. Experiences and results]. During the 12 month period June 1974-June 1975, 170 patients were advised on termination of pregnancy in the University Department of Obstetrics and Gynaecology, Mannheim, the results from 145 women are discussed. 74% of the women were married, the majority of whom were between 34-39 years old. 39% lived in bad, 40% in average and 21% in good social conditions. In 77 women the pregnancy was terminated after permission was granted from the health authority. In 43 patients the termination was not granted and 25 women did not return to the clinic when an expert opinion was demanded. In 50 women a definitive sterilisation was carried out at the same time as termination of the pregnancy.", "PMID": 955545}
{"id": "pubmed23n0032_1685", "title": "[Probability of malignant degeneration of ovarian tumors. Clinical study on the probability of malignant degeneration].", "content": "More than 600 cases of ovarian tumors were analyzed for histological type, age of patient, bilateral occurrence, mobility and malignant degeneration in restant ovaries. Tumors of germinal epithelial origin show the highest incidence (24%) of malignant degeneration, tumors of germ cells the lowest (3,5%). Incidence of malignant degeneration increases in relation to the age of the patient from 2% (and under 30 years) to 36,5% (60-70 years). Each form of tumor has its typical age peak, which is about 20 years higher for carcinomas than for their benign counterpart. Bilateral ovarian tumors are malignant in 29%, one-sided only in 13%. Among freely movable ovarian tumors, only 6% enclose a malignant tumor, among adhaerent ones 34%. The examination of the prognosis of granulosacell-tumors, dysgerminomas and teratoblastomas is based on a larger material.", "contents": "[Probability of malignant degeneration of ovarian tumors. Clinical study on the probability of malignant degeneration]. More than 600 cases of ovarian tumors were analyzed for histological type, age of patient, bilateral occurrence, mobility and malignant degeneration in restant ovaries. Tumors of germinal epithelial origin show the highest incidence (24%) of malignant degeneration, tumors of germ cells the lowest (3,5%). Incidence of malignant degeneration increases in relation to the age of the patient from 2% (and under 30 years) to 36,5% (60-70 years). Each form of tumor has its typical age peak, which is about 20 years higher for carcinomas than for their benign counterpart. Bilateral ovarian tumors are malignant in 29%, one-sided only in 13%. Among freely movable ovarian tumors, only 6% enclose a malignant tumor, among adhaerent ones 34%. The examination of the prognosis of granulosacell-tumors, dysgerminomas and teratoblastomas is based on a larger material.", "PMID": 955547}
{"id": "pubmed23n0032_1686", "title": "[Results of therapy in ovarian neoplasms].", "content": "35 patients with carcinoma of the ovary were treated at the University Hospital Department of Gynaecology and Obstetrics Ulm between January 1st, 1967 and March 1st, 1970. Stage Ia patients had the highest five year crude survival rate. Histological diagnosis did not play any role in these patients. A 20% crude survival rate was noted in stage III-patients. In this group of carcinoma of the ovary the best results with a survival rate of 38 months on average were found in those women who were treated by \"Second look method\".", "contents": "[Results of therapy in ovarian neoplasms]. 35 patients with carcinoma of the ovary were treated at the University Hospital Department of Gynaecology and Obstetrics Ulm between January 1st, 1967 and March 1st, 1970. Stage Ia patients had the highest five year crude survival rate. Histological diagnosis did not play any role in these patients. A 20% crude survival rate was noted in stage III-patients. In this group of carcinoma of the ovary the best results with a survival rate of 38 months on average were found in those women who were treated by \"Second look method\".", "PMID": 955548}
{"id": "pubmed23n0032_1687", "title": "[Heart injury due to blunt violence].", "content": "One case of non-penetrating injury of the heart is reported. The problem of the causal relation between accident and injury of the heart as well as the problems of the pathophysiological mechanisms of non-penetrating traumatic injury of the heart are discussed. The localisation of the non-penetrating traumatic injury, the volume and the energy of the object causing the injury as well as the preexisting degree of coronary sclerosis is of some importance for the severity of heart damages after non-penetrating traumatic injuries of the heart.", "contents": "[Heart injury due to blunt violence]. One case of non-penetrating injury of the heart is reported. The problem of the causal relation between accident and injury of the heart as well as the problems of the pathophysiological mechanisms of non-penetrating traumatic injury of the heart are discussed. The localisation of the non-penetrating traumatic injury, the volume and the energy of the object causing the injury as well as the preexisting degree of coronary sclerosis is of some importance for the severity of heart damages after non-penetrating traumatic injuries of the heart.", "PMID": 955551}
{"id": "pubmed23n0032_1688", "title": "[Urate nephrolithiasis. Cause of consequence?].", "content": "After World War II the incidence of urolithiasis increased consistently among the general population in this country. Nearly 25% of all examined renal calculi contain uric acid, sodium acid urate or ammonium acid urate as constituents. There are two peaks in lifespan of occurring urate stones: in the adolescence and in the age between 40 and 60 years. The following conditions are due to the formation of uric acid-containing stones: 1. Gout and primary hyperuricemia; 2. secondary hyperuricemia; 3. idiopathic cases with normal renal excretion of uric acid and normouricemia, but with a higher degree of acidity of the urine than normal considering the total renal excretion of acid products; 4. iatrogenic hyperuricemia during insufficient uricosuric therapy. Up to more than 30% of all the patients with recurrent formation of oxalate stones show a clear association with hyperuricemia, hyperuricosuria and increased renal excretion of calcium. In the presence of sodium urate a considerable promotion of precipitation of crystals consisting of calcium oxalate from a meta-stable solution may occur (so-called epitaxy). Frequently the existence of uric acid stones is without any symptoms. Modern views with regard to prophylactic procedures, diet, general and specific medical management including surgical intervention are presented.", "contents": "[Urate nephrolithiasis. Cause of consequence?]. After World War II the incidence of urolithiasis increased consistently among the general population in this country. Nearly 25% of all examined renal calculi contain uric acid, sodium acid urate or ammonium acid urate as constituents. There are two peaks in lifespan of occurring urate stones: in the adolescence and in the age between 40 and 60 years. The following conditions are due to the formation of uric acid-containing stones: 1. Gout and primary hyperuricemia; 2. secondary hyperuricemia; 3. idiopathic cases with normal renal excretion of uric acid and normouricemia, but with a higher degree of acidity of the urine than normal considering the total renal excretion of acid products; 4. iatrogenic hyperuricemia during insufficient uricosuric therapy. Up to more than 30% of all the patients with recurrent formation of oxalate stones show a clear association with hyperuricemia, hyperuricosuria and increased renal excretion of calcium. In the presence of sodium urate a considerable promotion of precipitation of crystals consisting of calcium oxalate from a meta-stable solution may occur (so-called epitaxy). Frequently the existence of uric acid stones is without any symptoms. Modern views with regard to prophylactic procedures, diet, general and specific medical management including surgical intervention are presented.", "PMID": 955552}
{"id": "pubmed23n0032_1689", "title": "[Worm-diseases].", "content": "Intestinal parasites are common among labourers from foreign countries and in immigrants. Some worm-infections like ascaridiasis or enterobiasis occur in children quite often even in the cold climates. In cases of ascaridiasis only a heavy infection causes clinical symptoms. The diagnosis is made through the detection of eggs in the stools, as all the other worm-infections are diagnosed from the stool-test. Piperazine-derivatives are highly effective against ascaris; another useful drug is Pyrantel-Pamoate. Toxocara canis, which affects normally only dogs, can occur occasionally in humans. Its larvae migrate in the body and cause granulomas. The main clinical symptom of threadworms (enterobiasis) is itching around the anus. This parasite can be eradicated through Pyrvinium-Pamoate. The first symptom a patient notices in cases of taeniasis are proglottides--parts of the worms--which are passed with the stools. The drug of choice against all kinds of tapeworms is Niclosamide. Hookworms are rampant in tropical countries. Heavy infections can cause severe anemias, especially in children. Bephenium-Naphthoate and Bitoscanate are effective drugs against this parasite. The whipworm (Trichuris trichura) is very common in the warm countries. It can be treated by Tiabendazole and nowadays also with Mebendazole, which is virtually atoxic. Infections with Strongyloides stercoralis are difficult to eradicate, as there are different ways of reinfection. In case an infection with strongyloides is accompanied by other serious diseases cachexia and even fatal outcome has been seen.", "contents": "[Worm-diseases]. Intestinal parasites are common among labourers from foreign countries and in immigrants. Some worm-infections like ascaridiasis or enterobiasis occur in children quite often even in the cold climates. In cases of ascaridiasis only a heavy infection causes clinical symptoms. The diagnosis is made through the detection of eggs in the stools, as all the other worm-infections are diagnosed from the stool-test. Piperazine-derivatives are highly effective against ascaris; another useful drug is Pyrantel-Pamoate. Toxocara canis, which affects normally only dogs, can occur occasionally in humans. Its larvae migrate in the body and cause granulomas. The main clinical symptom of threadworms (enterobiasis) is itching around the anus. This parasite can be eradicated through Pyrvinium-Pamoate. The first symptom a patient notices in cases of taeniasis are proglottides--parts of the worms--which are passed with the stools. The drug of choice against all kinds of tapeworms is Niclosamide. Hookworms are rampant in tropical countries. Heavy infections can cause severe anemias, especially in children. Bephenium-Naphthoate and Bitoscanate are effective drugs against this parasite. The whipworm (Trichuris trichura) is very common in the warm countries. It can be treated by Tiabendazole and nowadays also with Mebendazole, which is virtually atoxic. Infections with Strongyloides stercoralis are difficult to eradicate, as there are different ways of reinfection. In case an infection with strongyloides is accompanied by other serious diseases cachexia and even fatal outcome has been seen.", "PMID": 955553}
{"id": "pubmed23n0032_1690", "title": "[Therapy and prevention of chronic respiratory tract diseases].", "content": "196 patients suffering from chronic infections of the respiratory tract (bronchial asthma, spastic bronchitis, sinobronchitis, increased liability to infections during childhood) were treated with non-specific hyposensitization by Paspat as no improvements had been obtained with other methods. The patients were observed for at least one year after the termination of the therapy: Recovery in 62,2%, clear improvements in 23% of the cases; only 14,8% did not respond. The author can now rely on a total of 466 cases, who have been treated by this method for 21 years. The results obtained after the childhood were significantly improved by modifying the treatment and the remedies and by increasing the numbers of injections. Consequently, repeated applications of Paspat for treating and preventing chronic infections of the respiratory tract are considered a real progress.", "contents": "[Therapy and prevention of chronic respiratory tract diseases]. 196 patients suffering from chronic infections of the respiratory tract (bronchial asthma, spastic bronchitis, sinobronchitis, increased liability to infections during childhood) were treated with non-specific hyposensitization by Paspat as no improvements had been obtained with other methods. The patients were observed for at least one year after the termination of the therapy: Recovery in 62,2%, clear improvements in 23% of the cases; only 14,8% did not respond. The author can now rely on a total of 466 cases, who have been treated by this method for 21 years. The results obtained after the childhood were significantly improved by modifying the treatment and the remedies and by increasing the numbers of injections. Consequently, repeated applications of Paspat for treating and preventing chronic infections of the respiratory tract are considered a real progress.", "PMID": 955554}
{"id": "pubmed23n0032_1691", "title": "The influence of thyroid function on the diabetogenic action of triamcinolone in man. Glucose, insulin and growth hormone patterns after on oral glucose load.", "content": "In subjects with varying thyroid function (12 hypothyroid, 11 euthyroid and 12 hyperthyroid) an oral glucose tolerance test was performed without sensitization (OGTT) and after sensitizing with triamcinolone (TGTT). The blood glucose, serum immunoreactive insulin (IRI) and growth hormone (HGH) levels were assessed. In hypothyroid subjects a marked deterioration of the glucose tolerance was found during the TGTT as compared with OGTT, a reduction of the glucose disappearance index (GDI) and almost unchanged IRI values. The decrease of insulinogenic index (II) was not significant. In euthyroid subjects the decrease of glucose tolerance after triamcinolone was only mild, GDI values remained unaltered, IRI values and II increased. In hyperthyroid persons the decrease of glucose tolerance during TGTT was even less pronounced than in the euthyroid group, although the IRI value increased only on fasting. No significant differences of HGH pattern were observed in any group. In our patients the diabetogenic effect of triamcinolone was inversely related to the saturation with thyroid hormones and an increased response of insulin to glucose load was observed only in euthyroidism. In hyperthyroidism the participation of non-insular factors in the mechanism of maintaining the glucose tolerance after triamcinolone should be assumed.", "contents": "The influence of thyroid function on the diabetogenic action of triamcinolone in man. Glucose, insulin and growth hormone patterns after on oral glucose load. In subjects with varying thyroid function (12 hypothyroid, 11 euthyroid and 12 hyperthyroid) an oral glucose tolerance test was performed without sensitization (OGTT) and after sensitizing with triamcinolone (TGTT). The blood glucose, serum immunoreactive insulin (IRI) and growth hormone (HGH) levels were assessed. In hypothyroid subjects a marked deterioration of the glucose tolerance was found during the TGTT as compared with OGTT, a reduction of the glucose disappearance index (GDI) and almost unchanged IRI values. The decrease of insulinogenic index (II) was not significant. In euthyroid subjects the decrease of glucose tolerance after triamcinolone was only mild, GDI values remained unaltered, IRI values and II increased. In hyperthyroid persons the decrease of glucose tolerance during TGTT was even less pronounced than in the euthyroid group, although the IRI value increased only on fasting. No significant differences of HGH pattern were observed in any group. In our patients the diabetogenic effect of triamcinolone was inversely related to the saturation with thyroid hormones and an increased response of insulin to glucose load was observed only in euthyroidism. In hyperthyroidism the participation of non-insular factors in the mechanism of maintaining the glucose tolerance after triamcinolone should be assumed.", "PMID": 955557}
{"id": "pubmed23n0032_1692", "title": "Hypoglobulinemia in acromegaly.", "content": "We have observed an apparent hypoglobulinemia in 17 of 35 patients (48.6%) with acromegaly. This unexpected finding was persistent and reproducible up to six years for five acromegalic patients, and more than one year for nine other patients. Serum globulin was analyzed by three different methods, and the deficiency was most noticeable in the alpha globulin fraction (alpha1 greater than alpha2). When hypoglobulinemia occurred in control hospital in-patients (11%) it was associated with chronic or severe illnesses, and limited nutritional intake, but similar medical problems were absent in the acromegalic patients. There was no correlation of the hypoglobulinemia in the 35 acromegalic patients to their growth hormone (GH) concentration (r = 0.07), ages, sex, treatment status, or to the seriousness or duration of the acromegaly. The pathophysiology of the apparent hypoglobulinemia in acromegaly is unknown, but may be related to transport and/or disposal of excess growth hormone, or a defect in protein synthesis.", "contents": "Hypoglobulinemia in acromegaly. We have observed an apparent hypoglobulinemia in 17 of 35 patients (48.6%) with acromegaly. This unexpected finding was persistent and reproducible up to six years for five acromegalic patients, and more than one year for nine other patients. Serum globulin was analyzed by three different methods, and the deficiency was most noticeable in the alpha globulin fraction (alpha1 greater than alpha2). When hypoglobulinemia occurred in control hospital in-patients (11%) it was associated with chronic or severe illnesses, and limited nutritional intake, but similar medical problems were absent in the acromegalic patients. There was no correlation of the hypoglobulinemia in the 35 acromegalic patients to their growth hormone (GH) concentration (r = 0.07), ages, sex, treatment status, or to the seriousness or duration of the acromegaly. The pathophysiology of the apparent hypoglobulinemia in acromegaly is unknown, but may be related to transport and/or disposal of excess growth hormone, or a defect in protein synthesis.", "PMID": 955558}
{"id": "pubmed23n0032_1693", "title": "Circulating levels of immunoreactive parathyroid hormone in endemic genu valgum.", "content": "Circulating levels of immunoreactive parathyroid hormone (PTH) were estimated in fifteen normal subjects and twenty-five subjects suffering from the skeletal fluorosis, ten of whom had associated endemic genu valgum. Levels of PTH were high in all the subjects with fluorosis as compared to normals, but among fluorosis subjects, those who had endemic genu valgum had values strikingly higher than those without. It is suggested that secondary hyperparathyroidism may have a significant role to play in the pathogenesis of osteoporosis seen in patients with endemic genu valgum.", "contents": "Circulating levels of immunoreactive parathyroid hormone in endemic genu valgum. Circulating levels of immunoreactive parathyroid hormone (PTH) were estimated in fifteen normal subjects and twenty-five subjects suffering from the skeletal fluorosis, ten of whom had associated endemic genu valgum. Levels of PTH were high in all the subjects with fluorosis as compared to normals, but among fluorosis subjects, those who had endemic genu valgum had values strikingly higher than those without. It is suggested that secondary hyperparathyroidism may have a significant role to play in the pathogenesis of osteoporosis seen in patients with endemic genu valgum.", "PMID": 955559}
{"id": "pubmed23n0032_1694", "title": "Autoregulatory shift from fructolysis to lactate gluconeogenisis in rat hepatocyte suspensions. The problem of metabolic zonation of liver parenchyma.", "content": "Hepatocytes were isolated from fed rats with glucose and insulin and freom fasted rats with glucagon in all media in an attempt to obtain cells which might be fixed preferentially in either the glycolytic or gluconeogenic state. When tested enzymatically, both \"fed\" and fasted\" cells catalyzed glucose formation from lactate (gluconeogenesis) and lactate formation from fructose (fructolysis); lactate formation from glucose may have occurred in \"fed\" cells. Thus it was impossible, at least in the C3 part of the metabolic pathways between triosephosphate and pyruvate, to fix the hepatocytes in either metabolic state. The shift from glycolysis to gluconeogenesis could be investigated for the C3 part in \"fasted\" cells with fructose as the glycolytic and lactate as the gluconeogenic substrate. Lactate was first formed from fructose and later reutilized to a large extent. This reconsumption was blocked by the gluconeogenesis inhibitor quinolinate, both when tested enzymatically and radiochemically. Thus fructolysis was shifted to lactate gluconeogenesis. This shift at the assumed phosphoenolpyruvate/pyruvate cycle was autoregulatory, i.e. dependent on substrates and independent of circulating horomes. Maximal velocities and half saturating concentrations were determined for fructose and for lactate as substrates. The kinetic data obtained, especially the sigmoidal pattern of fructolysis, could nicely explain phenomenologically the rather sudden slow-down of lactate production and the shift to lactate consumption. The levels of the metabolites ATP, ADP, AMP, fructose bisphosphate and alanine, which control the enzymes of the assumed phosphoenolypyruvate/pyruvate cycle, were determined in the cytosol and in the mitochondria before and after the shift from fructose glycolysis to lactate gluconeogenesis. The changes observed could not explain the shift. Experiments with [14C] fructose plus unlabelled lactate and reciprocally, with unlabelled fructose plus [14C] lactate, clearly reveled that within the C3 part, glycolysis and gluconeogenesis were catalyzed simultaneously. The simultaneity of and the shift between fructolysis and gluconeogenesis by the liver cell suspension can best be explained by assuming two metabolically different types of hepatocytes rather than one type of hepatocyte with metabolically equal or different cell compartment. In vivo, the different types of hepatocytes would form a gluconeogenic and a glycolytic zone within the liver parenchyma. Since, under normal conditions, the size of these metabolic zones should remain unaltered, the shift from net glycolysis to net gluconeogenesis would be dependent primarily on substrate concentrations (autoregulation).", "contents": "Autoregulatory shift from fructolysis to lactate gluconeogenisis in rat hepatocyte suspensions. The problem of metabolic zonation of liver parenchyma. Hepatocytes were isolated from fed rats with glucose and insulin and freom fasted rats with glucagon in all media in an attempt to obtain cells which might be fixed preferentially in either the glycolytic or gluconeogenic state. When tested enzymatically, both \"fed\" and fasted\" cells catalyzed glucose formation from lactate (gluconeogenesis) and lactate formation from fructose (fructolysis); lactate formation from glucose may have occurred in \"fed\" cells. Thus it was impossible, at least in the C3 part of the metabolic pathways between triosephosphate and pyruvate, to fix the hepatocytes in either metabolic state. The shift from glycolysis to gluconeogenesis could be investigated for the C3 part in \"fasted\" cells with fructose as the glycolytic and lactate as the gluconeogenic substrate. Lactate was first formed from fructose and later reutilized to a large extent. This reconsumption was blocked by the gluconeogenesis inhibitor quinolinate, both when tested enzymatically and radiochemically. Thus fructolysis was shifted to lactate gluconeogenesis. This shift at the assumed phosphoenolpyruvate/pyruvate cycle was autoregulatory, i.e. dependent on substrates and independent of circulating horomes. Maximal velocities and half saturating concentrations were determined for fructose and for lactate as substrates. The kinetic data obtained, especially the sigmoidal pattern of fructolysis, could nicely explain phenomenologically the rather sudden slow-down of lactate production and the shift to lactate consumption. The levels of the metabolites ATP, ADP, AMP, fructose bisphosphate and alanine, which control the enzymes of the assumed phosphoenolypyruvate/pyruvate cycle, were determined in the cytosol and in the mitochondria before and after the shift from fructose glycolysis to lactate gluconeogenesis. The changes observed could not explain the shift. Experiments with [14C] fructose plus unlabelled lactate and reciprocally, with unlabelled fructose plus [14C] lactate, clearly reveled that within the C3 part, glycolysis and gluconeogenesis were catalyzed simultaneously. The simultaneity of and the shift between fructolysis and gluconeogenesis by the liver cell suspension can best be explained by assuming two metabolically different types of hepatocytes rather than one type of hepatocyte with metabolically equal or different cell compartment. In vivo, the different types of hepatocytes would form a gluconeogenic and a glycolytic zone within the liver parenchyma. Since, under normal conditions, the size of these metabolic zones should remain unaltered, the shift from net glycolysis to net gluconeogenesis would be dependent primarily on substrate concentrations (autoregulation).", "PMID": 955564}
{"id": "pubmed23n0032_1695", "title": "Plant microbody proteins, III. Labelling of the peroxisomal membrane protein SP-63 in vitro and in vivo.", "content": "Methods were developed to charcterize membranes and membrane components of leaf peroxisomes from Lens culinaris. While microbodies from etiolated young leaves exhibited an equilibrium density of 1.19 g/cm3, older leaves or leaves exposed to light for increasing periods of time contained microbodies banding at higher densities up to 1.235 g/cm3. Similar values were also found for the corresponding microbody membranes, which could be labelled with diazotized [35S]sulphanilic acid. Labelling was also performed using proteins extracted from the membranes. Their main structural protein (SP-63) was solubilized with sodium dodecylsulphate and labelled with fluorescent compounds as well as with diazotized [35S]sulphanilic acid or [3H]iodoacetic acid. These conversions greatly facilitate all analytical procedures, e.g. tracing the migration of SP-63 in gels or the movement in sucrose gradients containing sodium dodecylsulphate during zonal centrifugation. Also, labelling of SP-63 in vivo was accomplished when labelled amino acids were infused into etiolated leaves while exposing them to light.", "contents": "Plant microbody proteins, III. Labelling of the peroxisomal membrane protein SP-63 in vitro and in vivo. Methods were developed to charcterize membranes and membrane components of leaf peroxisomes from Lens culinaris. While microbodies from etiolated young leaves exhibited an equilibrium density of 1.19 g/cm3, older leaves or leaves exposed to light for increasing periods of time contained microbodies banding at higher densities up to 1.235 g/cm3. Similar values were also found for the corresponding microbody membranes, which could be labelled with diazotized [35S]sulphanilic acid. Labelling was also performed using proteins extracted from the membranes. Their main structural protein (SP-63) was solubilized with sodium dodecylsulphate and labelled with fluorescent compounds as well as with diazotized [35S]sulphanilic acid or [3H]iodoacetic acid. These conversions greatly facilitate all analytical procedures, e.g. tracing the migration of SP-63 in gels or the movement in sucrose gradients containing sodium dodecylsulphate during zonal centrifugation. Also, labelling of SP-63 in vivo was accomplished when labelled amino acids were infused into etiolated leaves while exposing them to light.", "PMID": 955565}
{"id": "pubmed23n0032_1696", "title": "Response of isolated rat hepatocytes to D-galactosamine and uridine.", "content": "Isolated rat hepatocytes preserved for several hours their contents of ATP, CTP, GTP, UTP and UDPG. Leakage of intracellular and membrane-bound enzymes remained in the range observed with the isolated perfused rat liver. D-Galactosamine led to a rapid decrease of the UTP and UDPG contents while ATP and GTP levels remained unaffected. The sum of acid-soluble uracil nucleotides increased immediately after the addition of D-galactosamine, indicating release of the UTP-mediated feed-back inhibition of pyrimidine nucleotide de novo biosynthesis. Addition of uridine to a hepatocyte suspension resulted in a rapid increase in the levels of uridylate derivatives; the concentrations of UTP and UDPG, previously depleted by D-galactosamine, were restored within 1 h. Isolated hepatocytes appear suitable for studies on the pathogenic sequence of events elicited by D-galactosamine and on the regulation of pyrimidine biosynthesis.", "contents": "Response of isolated rat hepatocytes to D-galactosamine and uridine. Isolated rat hepatocytes preserved for several hours their contents of ATP, CTP, GTP, UTP and UDPG. Leakage of intracellular and membrane-bound enzymes remained in the range observed with the isolated perfused rat liver. D-Galactosamine led to a rapid decrease of the UTP and UDPG contents while ATP and GTP levels remained unaffected. The sum of acid-soluble uracil nucleotides increased immediately after the addition of D-galactosamine, indicating release of the UTP-mediated feed-back inhibition of pyrimidine nucleotide de novo biosynthesis. Addition of uridine to a hepatocyte suspension resulted in a rapid increase in the levels of uridylate derivatives; the concentrations of UTP and UDPG, previously depleted by D-galactosamine, were restored within 1 h. Isolated hepatocytes appear suitable for studies on the pathogenic sequence of events elicited by D-galactosamine and on the regulation of pyrimidine biosynthesis.", "PMID": 955566}
{"id": "pubmed23n0032_1697", "title": "Crystallographic structural studies of a human Fc-fragment. I. An electron-density map at 4 A resolution and a partial model.", "content": "The crystal structure of a human Fc fragment was analysed at 4 A resolution. A partial interpretation of the electron-density map in terms of domain structure was possible. The molecule has the shape of a mickey mouse. The spherical domain was interpreted visually and by domain Patterson function interpretation as the CH3 dimer. This dimer resembles closely the CH1-CL dimer found in Fab structures. The ellipsoidal \"ears\" of the molecule represent the CH2 domains. They are widely separated from each other, but closely connected to CH3. Their tertiary structure must be different from CH1, as Patterson domain interpretations were unsuccessful. A chain tracing in CH2 was not yet possible.", "contents": "Crystallographic structural studies of a human Fc-fragment. I. An electron-density map at 4 A resolution and a partial model. The crystal structure of a human Fc fragment was analysed at 4 A resolution. A partial interpretation of the electron-density map in terms of domain structure was possible. The molecule has the shape of a mickey mouse. The spherical domain was interpreted visually and by domain Patterson function interpretation as the CH3 dimer. This dimer resembles closely the CH1-CL dimer found in Fab structures. The ellipsoidal \"ears\" of the molecule represent the CH2 domains. They are widely separated from each other, but closely connected to CH3. Their tertiary structure must be different from CH1, as Patterson domain interpretations were unsuccessful. A chain tracing in CH2 was not yet possible.", "PMID": 955567}
{"id": "pubmed23n0032_1698", "title": "[The synthesis of motilin, I: Preparation of the sequence fragments 9 - 22 of [13-norleucine]motilin and [13-leucine]motilin (author's transl)].", "content": "The syntheses of two suitably protected tetradecapeptides corresponding to the sequences 9 - 22 of [13-norlecucine] motilin [H-Glu(OBut)-Leu-Gln-Arg(HBr)-Nle-Glu(OBut)-Glu(OBut)-Lys(Boc)-Glu(OBut)-Arg(HBr-Asn-Lys(Boc)-Gly-Gln-OBu] and [13-leucine] motilin, respectively, are described. The two building blocks for the total syntheses of the motilin analogs were obtained by fragment-condensation of smaller units, prepared by stepwise procedure and corresponding to the sequences 18 - 22, 14 - 17, 12 - 13 (norleucine- and leucine-derivatives) and 9 - 11.", "contents": "[The synthesis of motilin, I: Preparation of the sequence fragments 9 - 22 of [13-norleucine]motilin and [13-leucine]motilin (author's transl)]. The syntheses of two suitably protected tetradecapeptides corresponding to the sequences 9 - 22 of [13-norlecucine] motilin [H-Glu(OBut)-Leu-Gln-Arg(HBr)-Nle-Glu(OBut)-Glu(OBut)-Lys(Boc)-Glu(OBut)-Arg(HBr-Asn-Lys(Boc)-Gly-Gln-OBu] and [13-leucine] motilin, respectively, are described. The two building blocks for the total syntheses of the motilin analogs were obtained by fragment-condensation of smaller units, prepared by stepwise procedure and corresponding to the sequences 18 - 22, 14 - 17, 12 - 13 (norleucine- and leucine-derivatives) and 9 - 11.", "PMID": 955568}
{"id": "pubmed23n0032_1699", "title": "[The synthesis of motilin, II: Preparation of the complete sequences of [13-norleucine] motilin and [13-leucine] motilin (author's transl)].", "content": "Syntheses of two analogs of the intestinal hormone motilin containing in position 13 norleucine and leucine, respectively, are described. For this purpose a suitably protected octapeptide-derivative, corresponding to the sequence 1-8, was prepared and condensed with the tetradecapeptides 9-22 of [13-norleucine]-motilin and [13-leucine] motilin (described in the preceding paper) to give to overall protectdd docosapeptides Boc-Phe-Val-Pro-IIe-Phe-Thr-(But)-Tyr(But)-Gly-Glu(OBut)-Leu-Gln-Arg-(HBr)-Nle-Glu(OBut)-Glu(OBut)-Lys(Boc)-Glu-(OBut)-Arg(HBr)-Asn-Lys(Boc)-Gly-Gln-OBut and its 13-leucine analog.", "contents": "[The synthesis of motilin, II: Preparation of the complete sequences of [13-norleucine] motilin and [13-leucine] motilin (author's transl)]. Syntheses of two analogs of the intestinal hormone motilin containing in position 13 norleucine and leucine, respectively, are described. For this purpose a suitably protected octapeptide-derivative, corresponding to the sequence 1-8, was prepared and condensed with the tetradecapeptides 9-22 of [13-norleucine]-motilin and [13-leucine] motilin (described in the preceding paper) to give to overall protectdd docosapeptides Boc-Phe-Val-Pro-IIe-Phe-Thr-(But)-Tyr(But)-Gly-Glu(OBut)-Leu-Gln-Arg-(HBr)-Nle-Glu(OBut)-Glu(OBut)-Lys(Boc)-Glu-(OBut)-Arg(HBr)-Asn-Lys(Boc)-Gly-Gln-OBut and its 13-leucine analog.", "PMID": 955569}
{"id": "pubmed23n0032_1700", "title": "[The synthesis of motilin, III: Purification and characterization of (13-norleucine) motilin and (13-leucine) motilin (author's transl)].", "content": "The preparation of the pure docosapeptide H-Phe-Val-Pro-I1e-Phe-Thr-Tyr-Gly-Glu-Leu-Gln-Arg-Nle-Glu-Glu-Lys-Glu-Arg-Asn-Lys-Gly-Gln-OH ([Nle13]motilin) and of the analogous [Leu13]motilin from the crude synthetic materials obtained after deblocking of the overall protected docosapeptide derivatives by means of trifluoroacetic acid is described. In a preliminary experiment the separation of crude [Nle13]-motilin into several components, some of which being indistinguishable by thin layer chromatography, was achieved by repeated ion-exchange chromatography on QAE-Sephadex A-25 and SP-Sephadex C-25. Subsequent characterization of some of the isolated side-products, using amino acid analysis as well as spectroscopic (UV, CD) and enzymatic methods, in comparison to the major product enabled conclusions on the reasons of their formation. The undesired formation of des-(Thr6 -Tyr7 Gly8)[Nle13] motilin and of [D-Phe5-Nle13]motilin could be avoided during the subsequent major synthesis of [Nle13] motilin and during the preparation of [Leu13] motilin by changing certain synthetic conditions and, respectively, by using a specially purified protected fragment of the sequence 1-8, being free of diastereomers. Single ion-exchange chromatography on SP-Sephadex C-25 was sufficient for the purification of the so obtained crude synthetic end-products.", "contents": "[The synthesis of motilin, III: Purification and characterization of (13-norleucine) motilin and (13-leucine) motilin (author's transl)]. The preparation of the pure docosapeptide H-Phe-Val-Pro-I1e-Phe-Thr-Tyr-Gly-Glu-Leu-Gln-Arg-Nle-Glu-Glu-Lys-Glu-Arg-Asn-Lys-Gly-Gln-OH ([Nle13]motilin) and of the analogous [Leu13]motilin from the crude synthetic materials obtained after deblocking of the overall protected docosapeptide derivatives by means of trifluoroacetic acid is described. In a preliminary experiment the separation of crude [Nle13]-motilin into several components, some of which being indistinguishable by thin layer chromatography, was achieved by repeated ion-exchange chromatography on QAE-Sephadex A-25 and SP-Sephadex C-25. Subsequent characterization of some of the isolated side-products, using amino acid analysis as well as spectroscopic (UV, CD) and enzymatic methods, in comparison to the major product enabled conclusions on the reasons of their formation. The undesired formation of des-(Thr6 -Tyr7 Gly8)[Nle13] motilin and of [D-Phe5-Nle13]motilin could be avoided during the subsequent major synthesis of [Nle13] motilin and during the preparation of [Leu13] motilin by changing certain synthetic conditions and, respectively, by using a specially purified protected fragment of the sequence 1-8, being free of diastereomers. Single ion-exchange chromatography on SP-Sephadex C-25 was sufficient for the purification of the so obtained crude synthetic end-products.", "PMID": 955570}
{"id": "pubmed23n0032_1701", "title": "A new semisynthesis of human insulin.", "content": "A new semisynthesis of human insulin starting from porcine insulin is described. NalphaA1, NalphaB1-di-Boc-desoctapeptide-(B23-30)-insulin bearing 6 free carboxyl groups is coupled with the suitably protected human insulin-(B23-30)-octapeptide. The preferred reaction site is the carboxyl group of ArgB22. Side products are eliminated by extensive chromatographic purification.", "contents": "A new semisynthesis of human insulin. A new semisynthesis of human insulin starting from porcine insulin is described. NalphaA1, NalphaB1-di-Boc-desoctapeptide-(B23-30)-insulin bearing 6 free carboxyl groups is coupled with the suitably protected human insulin-(B23-30)-octapeptide. The preferred reaction site is the carboxyl group of ArgB22. Side products are eliminated by extensive chromatographic purification.", "PMID": 955572}
{"id": "pubmed23n0032_1702", "title": "[Large scale purification of the acetylcholinesterase from bovine caudate nucleus by affinity chromatography (author's transl)].", "content": "The acetylcholinesterase from bovine caudate nucleus was solubilized with 0.6-0.8% Triton X-100. It was purified and freed from Triton X-100 by means of a two-fold affinity chromatography procedure. A simple three-step synthesis of the inhibitor with attached spacer arm used in affinity chromatography is described. Starting from 3 kg of caudate nucleus, nearly 11 mg of the purified acetylcholinesterase with a spec. act of 4250 U/mg (13 500-fold purification) could be obtained in an over-all yield of 49%. On gel electrophoresis the enzyme produces several enzymatically active glycoproteinbands of oligomeres. On sodium dodecylsulfate gel electrophoresis in the presence of a disulfide-reducing agent it yields a molecular weight of 72000 +/- 2000 for the smallest subunit. In the absence of a disulfide-reducing agent a band for a dimer besides the band for the monomer could be detected and a molecular weight of 142000 +/- 3000 was estimated for this species.", "contents": "[Large scale purification of the acetylcholinesterase from bovine caudate nucleus by affinity chromatography (author's transl)]. The acetylcholinesterase from bovine caudate nucleus was solubilized with 0.6-0.8% Triton X-100. It was purified and freed from Triton X-100 by means of a two-fold affinity chromatography procedure. A simple three-step synthesis of the inhibitor with attached spacer arm used in affinity chromatography is described. Starting from 3 kg of caudate nucleus, nearly 11 mg of the purified acetylcholinesterase with a spec. act of 4250 U/mg (13 500-fold purification) could be obtained in an over-all yield of 49%. On gel electrophoresis the enzyme produces several enzymatically active glycoproteinbands of oligomeres. On sodium dodecylsulfate gel electrophoresis in the presence of a disulfide-reducing agent it yields a molecular weight of 72000 +/- 2000 for the smallest subunit. In the absence of a disulfide-reducing agent a band for a dimer besides the band for the monomer could be detected and a molecular weight of 142000 +/- 3000 was estimated for this species.", "PMID": 955573}
{"id": "pubmed23n0032_1703", "title": "[Physico-chemical characterisation and crystallisation of immunoglobulins and their fragments: a contribution to the three dimensional structural determination of antibodies, III. Human immunoglobulin Kol, a myeloma protein of the type IgG, gamma2 kappa2, Gm (f)+. Comparative studies on dissolved and crystalline material (author's transl)].", "content": "Immunoglobulin Kol has been isolated and characterised as the intact molecule, which consists of an antigen binding part (Fab) and the C-terminal fragment (Fc). Crystals of the protein are suitable for X-ray diffraction resolution of the fine structure, down to atomic dimensions; but this does not permit the representation of the Fc-part of the molecule. The following studies were performed on the protein in solution before and after crystallisation; the results were the same in both cases, thus showing that crystallisation does not alter the molecule: After enzymic cleavage of the protein, the products were isolated and identified by immunological reactions and by analytical ultracentrifugation; the behaviour of the protein in disc electrophoresis following reductive cleavage was also studied.", "contents": "[Physico-chemical characterisation and crystallisation of immunoglobulins and their fragments: a contribution to the three dimensional structural determination of antibodies, III. Human immunoglobulin Kol, a myeloma protein of the type IgG, gamma2 kappa2, Gm (f)+. Comparative studies on dissolved and crystalline material (author's transl)]. Immunoglobulin Kol has been isolated and characterised as the intact molecule, which consists of an antigen binding part (Fab) and the C-terminal fragment (Fc). Crystals of the protein are suitable for X-ray diffraction resolution of the fine structure, down to atomic dimensions; but this does not permit the representation of the Fc-part of the molecule. The following studies were performed on the protein in solution before and after crystallisation; the results were the same in both cases, thus showing that crystallisation does not alter the molecule: After enzymic cleavage of the protein, the products were isolated and identified by immunological reactions and by analytical ultracentrifugation; the behaviour of the protein in disc electrophoresis following reductive cleavage was also studied.", "PMID": 955574}
{"id": "pubmed23n0032_1704", "title": "[Physico-chemical characterisation and crystallisation of immunoglobulins and their fragments: a contribution to the three-dimensional structural elucidation of antibodies, IV. The Fab fragment and the Fc fragment of human immunoglobulin Kol; isolation and crystallisation (author's transl)].", "content": "The X-ray fine structure analysis of intact crystalline immunoglobulin Kol indicates that the conformation of the Fab region differs essentially from that determined for the free Fab fragment. For this investigation, the free Fab fragment Kol was prepared and characterised, and crystalline preparations of the whole protein were obtained, that were suitable for an elucidation of atomic structure, and the appropriate measurements were made. In the crystal structure analysis of immunoglobulin Kol, it has not been possible to determine the Fc region. The Fc fragment was therefore separated from the whole molecule to facilitate its study. The isolation of this Fc fragment, its characterisation and subsequent crystallisation are described.", "contents": "[Physico-chemical characterisation and crystallisation of immunoglobulins and their fragments: a contribution to the three-dimensional structural elucidation of antibodies, IV. The Fab fragment and the Fc fragment of human immunoglobulin Kol; isolation and crystallisation (author's transl)]. The X-ray fine structure analysis of intact crystalline immunoglobulin Kol indicates that the conformation of the Fab region differs essentially from that determined for the free Fab fragment. For this investigation, the free Fab fragment Kol was prepared and characterised, and crystalline preparations of the whole protein were obtained, that were suitable for an elucidation of atomic structure, and the appropriate measurements were made. In the crystal structure analysis of immunoglobulin Kol, it has not been possible to determine the Fc region. The Fc fragment was therefore separated from the whole molecule to facilitate its study. The isolation of this Fc fragment, its characterisation and subsequent crystallisation are described.", "PMID": 955575}
{"id": "pubmed23n0032_1705", "title": "[Bis(monoacylglycero)phosphate--a marker lipid of secondary lysosomes? (AUTHOR'S TRANSL)].", "content": "Rats were treated with triton, dextran, silver and iron. The lysosomal fractions from their livers were isolated and the lipids analysed. (MAG)2P could be detected in every phospholipid fraction, accounting for 6-28 per cent of the total lipid phosphorus. The composition of the fatty acids from this acidic glycerophospholipid shows an extraordinarily high amount of polyenoic fatty acids (58-82 per cent of the total), of which docosahexaenoic acid is a main compound.", "contents": "[Bis(monoacylglycero)phosphate--a marker lipid of secondary lysosomes? (AUTHOR'S TRANSL)]. Rats were treated with triton, dextran, silver and iron. The lysosomal fractions from their livers were isolated and the lipids analysed. (MAG)2P could be detected in every phospholipid fraction, accounting for 6-28 per cent of the total lipid phosphorus. The composition of the fatty acids from this acidic glycerophospholipid shows an extraordinarily high amount of polyenoic fatty acids (58-82 per cent of the total), of which docosahexaenoic acid is a main compound.", "PMID": 955576}
{"id": "pubmed23n0032_1706", "title": "1,8-disubstituted analogues of (Ile5) and (Val5)angiotensin II: difference in potency and specificity of angiotensin II-antagonistic activity.", "content": "Depending on the species, position 5 in angiotensin II is occupied by isoleucine or valine. 1,8-disubstituted analogues of [Ile5]angiotensin II show distinct differences from the corresponding [Val5]angiotensin II analogues in the potency and specificity of their inhibitory action. The syntheses of new analogues are described.", "contents": "1,8-disubstituted analogues of (Ile5) and (Val5)angiotensin II: difference in potency and specificity of angiotensin II-antagonistic activity. Depending on the species, position 5 in angiotensin II is occupied by isoleucine or valine. 1,8-disubstituted analogues of [Ile5]angiotensin II show distinct differences from the corresponding [Val5]angiotensin II analogues in the potency and specificity of their inhibitory action. The syntheses of new analogues are described.", "PMID": 955577}
{"id": "pubmed23n0032_1707", "title": "Carboxypeptidase N from pig serum.", "content": "Carboxypeptidase N has been purified 865-fold from pig serum. The enzyme has a molecular weight of approximately 315 000. In the presence of dodecylsulfate and mercaptoethanol, it dissociates into three subunits of Mr = 90 000, 50 000, 30 000, respectively. The native enzyme and the subunit of Mr = 90 000 contain carbohydrate; no carbohydrate is found in the subunits of Mr = 50 000 and 30 000. Trypsin transforms carboxypeptidase N into a form having a smaller molecular weight and enhanced activity.", "contents": "Carboxypeptidase N from pig serum. Carboxypeptidase N has been purified 865-fold from pig serum. The enzyme has a molecular weight of approximately 315 000. In the presence of dodecylsulfate and mercaptoethanol, it dissociates into three subunits of Mr = 90 000, 50 000, 30 000, respectively. The native enzyme and the subunit of Mr = 90 000 contain carbohydrate; no carbohydrate is found in the subunits of Mr = 50 000 and 30 000. Trypsin transforms carboxypeptidase N into a form having a smaller molecular weight and enhanced activity.", "PMID": 955578}
{"id": "pubmed23n0032_1708", "title": "System links nurse call/locator, patient intercom, emergency call.", "content": "The communications system has improved patient care; nursing personnel have found it to be extremely valuable and easy to use; and physicians are pleased with it, because their patients are receiving better care and because communications are speeded when they call a floor for information or to give instructions. Installation and use of the system have proved that, with proper planning, hospitals can incorporate custom-designed systems and features into new construction at reasonable cost. Memorial Hospital now is in the process of proving that these innovations also can be incorporated into a remodeling and modernization program. Of course, among the key elements in the success of such endeavors are the willingness and the ability of equipment designers and manufacturers to satisfy specialized needs.", "contents": "System links nurse call/locator, patient intercom, emergency call. The communications system has improved patient care; nursing personnel have found it to be extremely valuable and easy to use; and physicians are pleased with it, because their patients are receiving better care and because communications are speeded when they call a floor for information or to give instructions. Installation and use of the system have proved that, with proper planning, hospitals can incorporate custom-designed systems and features into new construction at reasonable cost. Memorial Hospital now is in the process of proving that these innovations also can be incorporated into a remodeling and modernization program. Of course, among the key elements in the success of such endeavors are the willingness and the ability of equipment designers and manufacturers to satisfy specialized needs.", "PMID": 955614}
{"id": "pubmed23n0032_1709", "title": "Structural variability of human chromosome 9 in relation to its evolution.", "content": "Human chromosome 9 shows a high susceptibility for structural rearrangements, particularly pericentric inversions, which often are transmitted. Three types of pericentric inversions can be observed on No. 9: 1) Type I, showing the total constitutive heterochromatin in the short arm. 2) Type II with part of the C heterochromatin on the short arm, the rest located on the long arm proximal to the centromere. 3) Type III: a subtelocentric chromosome with part of the C heterochromatin in the very short arm and the rest located interstitially on the long arm. With these inversions as well as with other structural rearrangements, e.g. translocations, the break-points are located preferentially within the C heterochromatin or close to the heterochromatic-euchromatic junctions. These findings are in contrast to the findings in lymphocytes from 5 patients with fancomi's and after irradiation in vitro, reported in the literature. In lymphocytes break-points seem to be distributed more or less by chance. These observations together led us to speculate that human chromosome 9 primarily was an acrocentric chrosome; in morphology and at least in some functions similar to D- and G-group chromosomes. During evolution this acrocentric chromsome changed to a submetacentric one due to a pericentric inversion.", "contents": "Structural variability of human chromosome 9 in relation to its evolution. Human chromosome 9 shows a high susceptibility for structural rearrangements, particularly pericentric inversions, which often are transmitted. Three types of pericentric inversions can be observed on No. 9: 1) Type I, showing the total constitutive heterochromatin in the short arm. 2) Type II with part of the C heterochromatin on the short arm, the rest located on the long arm proximal to the centromere. 3) Type III: a subtelocentric chromosome with part of the C heterochromatin in the very short arm and the rest located interstitially on the long arm. With these inversions as well as with other structural rearrangements, e.g. translocations, the break-points are located preferentially within the C heterochromatin or close to the heterochromatic-euchromatic junctions. These findings are in contrast to the findings in lymphocytes from 5 patients with fancomi's and after irradiation in vitro, reported in the literature. In lymphocytes break-points seem to be distributed more or less by chance. These observations together led us to speculate that human chromosome 9 primarily was an acrocentric chrosome; in morphology and at least in some functions similar to D- and G-group chromosomes. During evolution this acrocentric chromsome changed to a submetacentric one due to a pericentric inversion.", "PMID": 955624}
{"id": "pubmed23n0032_1710", "title": "[Karyotype-phenotype correlation in a 46,Xdel(X) (p22) diagnosis (author's transl)].", "content": "In a patient with a height of 1.46 m, short neck and cubitus valgus the unbalanced karyotype 46,Xdel(X)(p22) was found. The mother of the proband has the balanced karyotype 46,Xt(X;15)(p22;p1). The mother is 1.56 m tall and has a short neck and cubitus valgus too. For this reason the deletion of the Xp22 band seems to cause the short stature of Turner patients. Our proband has had 2 pregnancies which ended as abortions in the 2nd and 4th month. As Giraud et al. (1974) have shown the deletion of the dark middle bande of the short X chromosome arm induces a slight dysgenesie of the gonade.", "contents": "[Karyotype-phenotype correlation in a 46,Xdel(X) (p22) diagnosis (author's transl)]. In a patient with a height of 1.46 m, short neck and cubitus valgus the unbalanced karyotype 46,Xdel(X)(p22) was found. The mother of the proband has the balanced karyotype 46,Xt(X;15)(p22;p1). The mother is 1.56 m tall and has a short neck and cubitus valgus too. For this reason the deletion of the Xp22 band seems to cause the short stature of Turner patients. Our proband has had 2 pregnancies which ended as abortions in the 2nd and 4th month. As Giraud et al. (1974) have shown the deletion of the dark middle bande of the short X chromosome arm induces a slight dysgenesie of the gonade.", "PMID": 955625}
{"id": "pubmed23n0032_1711", "title": "47,+(9q-) in unrelated three children with plasma growth hormone deficiency.", "content": "Marker chromosomes carried by unrelated 3 cases were identified as a part of No. 9 chromosome through the analysis of the chromatid fine structure after trypsin-giemsa treatment. They showed characteristic features of that 9p trisomic syndrome which were described by Rethor\u00e9 et al. (1973). In addition to those features, some clinical and laboratory findings on growth hormon deficiency were disclosed in this report.", "contents": "47,+(9q-) in unrelated three children with plasma growth hormone deficiency. Marker chromosomes carried by unrelated 3 cases were identified as a part of No. 9 chromosome through the analysis of the chromatid fine structure after trypsin-giemsa treatment. They showed characteristic features of that 9p trisomic syndrome which were described by Rethor\u00e9 et al. (1973). In addition to those features, some clinical and laboratory findings on growth hormon deficiency were disclosed in this report.", "PMID": 955626}
{"id": "pubmed23n0032_1712", "title": "Serum immunoglobulin M, G, and A concentration levels in Turner's syndrome compared with normal women and men.", "content": "Serum concentrations of immunoglobulin G (IgG), immunoglobulin M (IgM) and immunoglobulin A (IgA) were determined in 15 women with a lack of X chromsome material (Turner's syndrome), and compared with the immunoglobulin concentrations in normal men and women. Further, the investigation is supplemented by a comparison of normal women and the Turner group matched according to age. The serum concentrations of IgG and IgA in women with Turner's syndrome were very close to the concentration in serum from normal men, whereas the concentration of IgM was significantly lower. Compared to normal women the concentrations of IgG and IgM were significantly lower, and the concentration of IgA significantly higher in the Turner group. Whether these differences in serum immunoglobulins are determined by hormonal factors or under direct genetic control linked to the X chromosomes, is discussed.", "contents": "Serum immunoglobulin M, G, and A concentration levels in Turner's syndrome compared with normal women and men. Serum concentrations of immunoglobulin G (IgG), immunoglobulin M (IgM) and immunoglobulin A (IgA) were determined in 15 women with a lack of X chromsome material (Turner's syndrome), and compared with the immunoglobulin concentrations in normal men and women. Further, the investigation is supplemented by a comparison of normal women and the Turner group matched according to age. The serum concentrations of IgG and IgA in women with Turner's syndrome were very close to the concentration in serum from normal men, whereas the concentration of IgM was significantly lower. Compared to normal women the concentrations of IgG and IgM were significantly lower, and the concentration of IgA significantly higher in the Turner group. Whether these differences in serum immunoglobulins are determined by hormonal factors or under direct genetic control linked to the X chromosomes, is discussed.", "PMID": 955627}
{"id": "pubmed23n0032_1713", "title": "Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells.", "content": "The synthesis of the most common type I of collagen was proven in human amniotic fluid cell culture with fibroblast morphology in the cells as well as in the culture medium. Mesenchymal origin of long term cultivated amniotic fluid cells is indicated and the possibility of prenatal investigation of hereditary disorders of connective tissue is pointed out.", "contents": "Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells. The synthesis of the most common type I of collagen was proven in human amniotic fluid cell culture with fibroblast morphology in the cells as well as in the culture medium. Mesenchymal origin of long term cultivated amniotic fluid cells is indicated and the possibility of prenatal investigation of hereditary disorders of connective tissue is pointed out.", "PMID": 955628}
{"id": "pubmed23n0032_1714", "title": "The trisomy 18 syndrome with an E/G translocation.", "content": "An infant with a typical Edwards syndrome and a modal chromosome number of 46 is reported. In all cells analyzed one chromosome G was missing and an additional chromosome similar to a pair No. 16 was present. The phenotype of the child indicates that the extra element is a translocation between G and 18 chromosomes as in one case described previously.", "contents": "The trisomy 18 syndrome with an E/G translocation. An infant with a typical Edwards syndrome and a modal chromosome number of 46 is reported. In all cells analyzed one chromosome G was missing and an additional chromosome similar to a pair No. 16 was present. The phenotype of the child indicates that the extra element is a translocation between G and 18 chromosomes as in one case described previously.", "PMID": 955629}
{"id": "pubmed23n0032_1715", "title": "A female infant with monosomy 21.", "content": "A female infant with total monosomy 21 identified by Q banding is described. The main clinical features were hypertonia, prominent occiput, hypertelorism, antimongoloid slant of the eyes, broad nose, \"antimongoloid\", character of dermatoglyphics. Both parents are phenotypically as well as karyotypically normal.", "contents": "A female infant with monosomy 21. A female infant with total monosomy 21 identified by Q banding is described. The main clinical features were hypertonia, prominent occiput, hypertelorism, antimongoloid slant of the eyes, broad nose, \"antimongoloid\", character of dermatoglyphics. Both parents are phenotypically as well as karyotypically normal.", "PMID": 955630}
{"id": "pubmed23n0032_1716", "title": "9p trisomy identified by Giemsa-11.", "content": "The Giemsa-11 technique specifically stains the secondary constriction region of chromosome 9. We report cytologic evidence for 9p trisomy in a 4 1/2-year-old girl with moderate retardation and multiple anomalies, using the Giemsa-11 technique.", "contents": "9p trisomy identified by Giemsa-11. The Giemsa-11 technique specifically stains the secondary constriction region of chromosome 9. We report cytologic evidence for 9p trisomy in a 4 1/2-year-old girl with moderate retardation and multiple anomalies, using the Giemsa-11 technique.", "PMID": 955631}
{"id": "pubmed23n0032_1717", "title": "Studies of haptoglobin and transferrin types in four castes of the Panjab, Northern India.", "content": "Serum samples from 197 individuals belonging to the caste groups of Brahmin (37), Bania (39), Khatri (73) and Jat (48) were electrophoretically examined for the haptoglobin and transferrin systems. Intercaste variation was considered and comparisons made between these North Indian populations and those in other parts of the Indian subcontinent. A single Khatri individual was typed CB.", "contents": "Studies of haptoglobin and transferrin types in four castes of the Panjab, Northern India. Serum samples from 197 individuals belonging to the caste groups of Brahmin (37), Bania (39), Khatri (73) and Jat (48) were electrophoretically examined for the haptoglobin and transferrin systems. Intercaste variation was considered and comparisons made between these North Indian populations and those in other parts of the Indian subcontinent. A single Khatri individual was typed CB.", "PMID": 955633}
{"id": "pubmed23n0032_1718", "title": "On the geographical variability of the red cell PGM1 and acid phosphatase gene frequencies.", "content": "Based on the hitherto published population data of the human red cell PGM1 and acid phosphatase polymorphisms, the geographical distributions of their gene frequencies were analyzed. As far as the acid phosphatase alleles are concerned, a marked geographical gradient was found as the Pa and Pb alleles showed significant correlations with the mean annual temperatures of the various human biotopes (Pa:r = -0.706; Pb:r = +0.812). Against that, the world distribution of the PGM1 alleles did not show a comparable correlation (PGM1 1:R = +0.063; PGM2 1:R = -0.063). The possible reasons for the distribution pattern of the acid phosphatase alleles are discussed.", "contents": "On the geographical variability of the red cell PGM1 and acid phosphatase gene frequencies. Based on the hitherto published population data of the human red cell PGM1 and acid phosphatase polymorphisms, the geographical distributions of their gene frequencies were analyzed. As far as the acid phosphatase alleles are concerned, a marked geographical gradient was found as the Pa and Pb alleles showed significant correlations with the mean annual temperatures of the various human biotopes (Pa:r = -0.706; Pb:r = +0.812). Against that, the world distribution of the PGM1 alleles did not show a comparable correlation (PGM1 1:R = +0.063; PGM2 1:R = -0.063). The possible reasons for the distribution pattern of the acid phosphatase alleles are discussed.", "PMID": 955634}
{"id": "pubmed23n0032_1719", "title": "Superoxide dismutase variants in Newfoundland--a gene from Scandinavia?", "content": "An electrophoretic survey of variant red cell enzyme phenotypes in an isolated community in Newfoundland gave gene frequencies in marked contrast to those found in the capital, St. John's. In particular, a variant of red cell superoxide dismutase (SOD) gave a high variant frequency. Possible origins of the variant SOD allele are discussed.", "contents": "Superoxide dismutase variants in Newfoundland--a gene from Scandinavia? An electrophoretic survey of variant red cell enzyme phenotypes in an isolated community in Newfoundland gave gene frequencies in marked contrast to those found in the capital, St. John's. In particular, a variant of red cell superoxide dismutase (SOD) gave a high variant frequency. Possible origins of the variant SOD allele are discussed.", "PMID": 955635}
{"id": "pubmed23n0032_1720", "title": "Human red cell acid phosphatase: quantitative evidence of a silent gene PO, and a Danish population study.", "content": "In a forensic case of disputed paternity an apparent mother/child incompatibility with respect to red cell acid phosphatase was found, the mother appearing as type A and the child as type B. Determination of electrophoretic type and of acid phosphatase activity in 8 of the family members strongly suggested the presence of a silent gene PO in 4 of the individuals. The phosphatase levels in the four heterozygotes were about half the values expected from normal values determined in 100 healthy adults representing the different phenotypes. The distribution of red cell acid phosphatase types in 3,735 unrelated Danish adults and in 1,109 mother/child pairs is reported; gene frequencies Pa = 0.369, Pb = 0.566 and Pc = 0.065. The PO gene frequency was roughly estimated as 0.001. Results are reported on the application of the red cell acid phosphatase system to 300 2-men cases of disputed paternity.", "contents": "Human red cell acid phosphatase: quantitative evidence of a silent gene PO, and a Danish population study. In a forensic case of disputed paternity an apparent mother/child incompatibility with respect to red cell acid phosphatase was found, the mother appearing as type A and the child as type B. Determination of electrophoretic type and of acid phosphatase activity in 8 of the family members strongly suggested the presence of a silent gene PO in 4 of the individuals. The phosphatase levels in the four heterozygotes were about half the values expected from normal values determined in 100 healthy adults representing the different phenotypes. The distribution of red cell acid phosphatase types in 3,735 unrelated Danish adults and in 1,109 mother/child pairs is reported; gene frequencies Pa = 0.369, Pb = 0.566 and Pc = 0.065. The PO gene frequency was roughly estimated as 0.001. Results are reported on the application of the red cell acid phosphatase system to 300 2-men cases of disputed paternity.", "PMID": 955636}
{"id": "pubmed23n0032_1721", "title": "Pancreatic amylase polymorphism: another example of a distinctive gene frequency among Sardinians.", "content": "The authors investigate the distribution of electrophoretic patterns of pancreatic amylase in 319 Sardinians and in 476 blood donors living in Naples. The results obtained show a difference in the phenotype frequency of the Amy2 duplication variant between Sardinians (1.25%) and Neapolitans (5.25%) which is statistically significant (p less than 0.01). This further confirms that Sardinians show peculiar ethnic characteristics as compared to other Caucasian groups, even to those living in the Mediterranean area.", "contents": "Pancreatic amylase polymorphism: another example of a distinctive gene frequency among Sardinians. The authors investigate the distribution of electrophoretic patterns of pancreatic amylase in 319 Sardinians and in 476 blood donors living in Naples. The results obtained show a difference in the phenotype frequency of the Amy2 duplication variant between Sardinians (1.25%) and Neapolitans (5.25%) which is statistically significant (p less than 0.01). This further confirms that Sardinians show peculiar ethnic characteristics as compared to other Caucasian groups, even to those living in the Mediterranean area.", "PMID": 955637}
{"id": "pubmed23n0032_1722", "title": "The G syndrome. A four-generation family study.", "content": "A male infant with hypertelorism, hypospadias, swallowing difficulties with tendency to regurgitation and cough, high arched palate, and a delicate voice, consistent with the G syndrome, is reported. In the family the same symptoms in addition to cleft lip and palate were known in several family members through four generations. In the females only slight manifestations of the syndrome were found, and in the males variable expression of symptoms was observed. Autosomal dominant inheritance is likely, but X-linked inheritance cannot be ruled out.", "contents": "The G syndrome. A four-generation family study. A male infant with hypertelorism, hypospadias, swallowing difficulties with tendency to regurgitation and cough, high arched palate, and a delicate voice, consistent with the G syndrome, is reported. In the family the same symptoms in addition to cleft lip and palate were known in several family members through four generations. In the females only slight manifestations of the syndrome were found, and in the males variable expression of symptoms was observed. Autosomal dominant inheritance is likely, but X-linked inheritance cannot be ruled out.", "PMID": 955638}
{"id": "pubmed23n0032_1723", "title": "On the genetics and population genetics of Gm(4).", "content": "Investigations on 70 German families with 142 children confirmed the autosomal-dominant inheritance of Gm(4). However, in our material as well as in the materials published so far, Gm(4) X Gm(-4) parental combinations show clear surplusses of Gm(4) and deficits of Gm(-4) children, which might indicate prenatal selection. The world distribution of Gm4 alleles reveals a remarkable racial heterogeneity. Furthermore, marked North-South distribution gradients in Caucasoids and Mongoloids were found. The genetical and population genetical observations are discussed.", "contents": "On the genetics and population genetics of Gm(4). Investigations on 70 German families with 142 children confirmed the autosomal-dominant inheritance of Gm(4). However, in our material as well as in the materials published so far, Gm(4) X Gm(-4) parental combinations show clear surplusses of Gm(4) and deficits of Gm(-4) children, which might indicate prenatal selection. The world distribution of Gm4 alleles reveals a remarkable racial heterogeneity. Furthermore, marked North-South distribution gradients in Caucasoids and Mongoloids were found. The genetical and population genetical observations are discussed.", "PMID": 955639}
{"id": "pubmed23n0032_1724", "title": "Two kindred with the rare dominant inhibitor of the lutheran and p1 red cell antigens.", "content": "Two kindred each showing the dominant inheritance of the rare phenotype lu(a-b-) in the Lutheran blood group system are recorded. The pedigrees illustrate the expected upset in the occurrence of the P1 antigen in the Lu(a-b-) members. A third Lu(a-b-) propositus of the dominant type was found after testing a further 3,197 donors on the East Anglian panel. The phenotype Lu(a-b-) may not be quite as rare as previously supposed.", "contents": "Two kindred with the rare dominant inhibitor of the lutheran and p1 red cell antigens. Two kindred each showing the dominant inheritance of the rare phenotype lu(a-b-) in the Lutheran blood group system are recorded. The pedigrees illustrate the expected upset in the occurrence of the P1 antigen in the Lu(a-b-) members. A third Lu(a-b-) propositus of the dominant type was found after testing a further 3,197 donors on the East Anglian panel. The phenotype Lu(a-b-) may not be quite as rare as previously supposed.", "PMID": 955641}
{"id": "pubmed23n0032_1725", "title": "Population genetic studies in kerala and the nilgiris (South West India).", "content": "A total of nearly 1,000 persons belonging to a number of caste, religious and tribal groupings in Kerala and the Nilgiri Hills of South India have been tested for genetic variation in 4 blood group, 5 serum protein and 17 enzyme systems as well as haemoglobin. The distribution of blood groups, serum protein and enzyme groups is similar to that reported for other South Indian populations. Abnormal  haemoglobins were detected in several populations. Abnormal haemoglobins were detected in several populations, HbS being present in more than 20% of the Irula and Kurumba in the Nilgiri Hills. In the Kerala populations there were 4 examples of Hb AD and 1 of Hb AE. Genetic distance estimates using the gene frequency data indicate that the closest groups are the Nayar and Izhava and the Brahmin and Nayar. The tribal populations are approximately twice as far from the Nayar as they are from the Izhava. The Todas of the Nilgiri Hills are somewhat closer to the Brahmin of Kerala than they are to the other tribal populations.", "contents": "Population genetic studies in kerala and the nilgiris (South West India). A total of nearly 1,000 persons belonging to a number of caste, religious and tribal groupings in Kerala and the Nilgiri Hills of South India have been tested for genetic variation in 4 blood group, 5 serum protein and 17 enzyme systems as well as haemoglobin. The distribution of blood groups, serum protein and enzyme groups is similar to that reported for other South Indian populations. Abnormal  haemoglobins were detected in several populations. Abnormal haemoglobins were detected in several populations, HbS being present in more than 20% of the Irula and Kurumba in the Nilgiri Hills. In the Kerala populations there were 4 examples of Hb AD and 1 of Hb AE. Genetic distance estimates using the gene frequency data indicate that the closest groups are the Nayar and Izhava and the Brahmin and Nayar. The tribal populations are approximately twice as far from the Nayar as they are from the Izhava. The Todas of the Nilgiri Hills are somewhat closer to the Brahmin of Kerala than they are to the other tribal populations.", "PMID": 955642}
{"id": "pubmed23n0032_1726", "title": "The study of genetic variation in Nigeria. II. The genetics of polydactyly.", "content": "The analysis of polydactyly in the Nigerian population has revealed a frequency of 22.78 per thousand, with slight variations from one region of the country to another. All polydactyly was post-axial and most was of type B. Although we expected this frequency to be the same in both males and females, we observed that females have a significantly lower frequency (17.92 per thousand) than the males (27.08 per thousand). This condition appears to be inherited as an autosomal dominant gene with a penetrance of 64.9%, and highly variable expressivity. There does not seem to be any phenotypic difference between individuals who are homozygous and those who are heterozygous for the gene.", "contents": "The study of genetic variation in Nigeria. II. The genetics of polydactyly. The analysis of polydactyly in the Nigerian population has revealed a frequency of 22.78 per thousand, with slight variations from one region of the country to another. All polydactyly was post-axial and most was of type B. Although we expected this frequency to be the same in both males and females, we observed that females have a significantly lower frequency (17.92 per thousand) than the males (27.08 per thousand). This condition appears to be inherited as an autosomal dominant gene with a penetrance of 64.9%, and highly variable expressivity. There does not seem to be any phenotypic difference between individuals who are homozygous and those who are heterozygous for the gene.", "PMID": 955643}
{"id": "pubmed23n0032_1727", "title": "Human saliva esterases: genetic studies.", "content": "Esterases from human saliva have been demonstrated by the zymogram technique. Three phenotypes were found in region 1. Family and population studies suggested that these phenotypes are the products of an autosomal locus with two alleles, Set-1F and Set-1S. Region 1 esterases are carboxylesterases.", "contents": "Human saliva esterases: genetic studies. Esterases from human saliva have been demonstrated by the zymogram technique. Three phenotypes were found in region 1. Family and population studies suggested that these phenotypes are the products of an autosomal locus with two alleles, Set-1F and Set-1S. Region 1 esterases are carboxylesterases.", "PMID": 955645}
{"id": "pubmed23n0032_1728", "title": "Lymphocytocidal lymphocyte trapping by human lymph node cells: a tissue culture-ultrastructural study.", "content": "An ultrastructural study was carried out on 25 lymphocyte-trapping cells selected from tissue cultures of human axillary lymph nodes. The trapping cells contained several hundred intravacuolar lymphocytes, most of which showed degenerative changes. The principal findings are: (a) a braod spectrum of lymphocyte degeneration; (b) a consistent pattern of lymphocyte degeneration beginning with perinuclear vacuoles and ending with breakdown of the nuclear envelope; (c) the viable lymphocytes tended to be located in a  juxtanuclear region; (d) a lysosomal relationship was suggested for lymphocyte degeneration but not for lymphocyte  trapping; and (e) degeneration of the trapping cell, or lymphocytes associated with other cells, was not observed. The sequence of degenerative  changes differs from those reported for several classes of lymphocytocidal agents. There were no morphologic properties of the trapping cell which served to identify it more specifically. The findings, together with previous time-lapse film observations, warrant further investigation of the hypothesis that lymphocytocidal lymphocyte trapping may be involved in the control of lymphocyte populations.", "contents": "Lymphocytocidal lymphocyte trapping by human lymph node cells: a tissue culture-ultrastructural study. An ultrastructural study was carried out on 25 lymphocyte-trapping cells selected from tissue cultures of human axillary lymph nodes. The trapping cells contained several hundred intravacuolar lymphocytes, most of which showed degenerative changes. The principal findings are: (a) a braod spectrum of lymphocyte degeneration; (b) a consistent pattern of lymphocyte degeneration beginning with perinuclear vacuoles and ending with breakdown of the nuclear envelope; (c) the viable lymphocytes tended to be located in a  juxtanuclear region; (d) a lysosomal relationship was suggested for lymphocyte degeneration but not for lymphocyte  trapping; and (e) degeneration of the trapping cell, or lymphocytes associated with other cells, was not observed. The sequence of degenerative  changes differs from those reported for several classes of lymphocytocidal agents. There were no morphologic properties of the trapping cell which served to identify it more specifically. The findings, together with previous time-lapse film observations, warrant further investigation of the hypothesis that lymphocytocidal lymphocyte trapping may be involved in the control of lymphocyte populations.", "PMID": 955646}
{"id": "pubmed23n0032_1729", "title": "Occurrence of minute ring-shaped nucleoprotein particles in culture media conditioned by mammalian cells.", "content": "Conditioned media of 21 mammalian cell lines have been examined by electron microscopic and biochemical techniques for the presence of a minute ring-shaped nucleoprotein particle (RSP). Electron microscopy showed the presence of RSP in 20 of the 21 cell lines tested. Comparative analyses of sex cell cultures for radioactive RSP, following 3H-thymidine incorporation, suggested that quantitative differences exist in the amount of RSP detectable in conditioned media of normal and pathologic cells. The cell lines tested included cells of different morphologies, origin and function", "contents": "Occurrence of minute ring-shaped nucleoprotein particles in culture media conditioned by mammalian cells. Conditioned media of 21 mammalian cell lines have been examined by electron microscopic and biochemical techniques for the presence of a minute ring-shaped nucleoprotein particle (RSP). Electron microscopy showed the presence of RSP in 20 of the 21 cell lines tested. Comparative analyses of sex cell cultures for radioactive RSP, following 3H-thymidine incorporation, suggested that quantitative differences exist in the amount of RSP detectable in conditioned media of normal and pathologic cells. The cell lines tested included cells of different morphologies, origin and function", "PMID": 955647}
{"id": "pubmed23n0032_1730", "title": "Incorporation and uptake of thymidine and uridine by HeLa cells: effect of DNA extracts prepared from normal human fibroblasts.", "content": "The incorporation and uptake of (3H) thymidine into HeLa cells markedly decreased in the presence of nuclear homogenates and DNA extracts that have been derived from normal diploid cell cultures. On the other hand, uridine uptake and incorporation were stimulated under the same conditions. The inhibition could be reversed immediately upon removal of the exogenous fraction from the culture medium. The inhibitory properties of the extracts are propagated by excreted cellular components as well as after DNAase treatment. The inhibitory factor is thermostable, resistant to pronase treatment, and seems to be related to nucleic acid.", "contents": "Incorporation and uptake of thymidine and uridine by HeLa cells: effect of DNA extracts prepared from normal human fibroblasts. The incorporation and uptake of (3H) thymidine into HeLa cells markedly decreased in the presence of nuclear homogenates and DNA extracts that have been derived from normal diploid cell cultures. On the other hand, uridine uptake and incorporation were stimulated under the same conditions. The inhibition could be reversed immediately upon removal of the exogenous fraction from the culture medium. The inhibitory properties of the extracts are propagated by excreted cellular components as well as after DNAase treatment. The inhibitory factor is thermostable, resistant to pronase treatment, and seems to be related to nucleic acid.", "PMID": 955648}
{"id": "pubmed23n0032_1731", "title": "Comparative study of extracellular amino acids in culture of human liver and fibroblastic cells.", "content": "Amino acid concentration are studied in the extracellular media of ten series of human fibroblast and liver cell monolayer cultures. These two cell types consume and produce ostensively the same amino acids. Among the nonessential amino acids, the most significant variations involve serine and aspartate which are decreased; alpha-alanine, glutamate, ornithine and proline are, on the contrary, increased. Among the essential amino acids, leucine, isoleucine and glutamine are preferentially decreased. The variations of some amino acids are correlated with the cell density. The interrelations which may exist between the variations of these different amino acids are discussed. Furthermore, the glycolytic acitivity of the cells studied is very high: 85% of glucose consumed is found in the form of lactate.", "contents": "Comparative study of extracellular amino acids in culture of human liver and fibroblastic cells. Amino acid concentration are studied in the extracellular media of ten series of human fibroblast and liver cell monolayer cultures. These two cell types consume and produce ostensively the same amino acids. Among the nonessential amino acids, the most significant variations involve serine and aspartate which are decreased; alpha-alanine, glutamate, ornithine and proline are, on the contrary, increased. Among the essential amino acids, leucine, isoleucine and glutamine are preferentially decreased. The variations of some amino acids are correlated with the cell density. The interrelations which may exist between the variations of these different amino acids are discussed. Furthermore, the glycolytic acitivity of the cells studied is very high: 85% of glucose consumed is found in the form of lactate.", "PMID": 955649}
{"id": "pubmed23n0032_1732", "title": "Effect of prolactin on maintenance of prelactating human mammary gland in vitro.", "content": "Human mammary tissue from a female at the end of the second trimester of pregnancy was studied in organ culture in a chemically defined medium. Sampling was carried out at 1, 2 and 3 weeks. Without hormones, there was nearly total lobuloalveolar degeneration in all specimens at all times. Addition of insulin, hydrocortisone and ovine prolactin, in combination at a concentration of 5 mug per ml each, did not affect the extent of degeneration. Raising the concentration of prolactin to 50 mug per ml resulted in greatly improved lobulo-alveolar maintenance in all specimens and continued epithelial cell DNA synthesis for up to 3 weeks in vitro.", "contents": "Effect of prolactin on maintenance of prelactating human mammary gland in vitro. Human mammary tissue from a female at the end of the second trimester of pregnancy was studied in organ culture in a chemically defined medium. Sampling was carried out at 1, 2 and 3 weeks. Without hormones, there was nearly total lobuloalveolar degeneration in all specimens at all times. Addition of insulin, hydrocortisone and ovine prolactin, in combination at a concentration of 5 mug per ml each, did not affect the extent of degeneration. Raising the concentration of prolactin to 50 mug per ml resulted in greatly improved lobulo-alveolar maintenance in all specimens and continued epithelial cell DNA synthesis for up to 3 weeks in vitro.", "PMID": 955650}
{"id": "pubmed23n0032_1733", "title": "The effect of cytophilic IgG2 on phagocytosis by ovine polymorphonuclear leucocytes.", "content": "Non-lactating, multiparous ewes were immunized either by subcutaneous infection with live Staphylococcus aureus (seventeen ewes) or by intramuscular injections of a killed S. aureus-oil adjuvant vaccine (seventeen ewes). Polymorphs which were subsequently collected from the mammary glands of the animals were used in in vitro phagocytosis assays against Pseudomonas sp. or S. aureus. There was no difference between polymorphs from the two groups of ewes in their ability to phagocytose Pseudomonas organisms. Polymorphs from the infected ewes showed significant phagocytic superiority over cells from ewes given the killed vaccine when S. aureus was the target organism. This phagocytic superiority could be abrogated by removal of cytophilic immunoglobulin from polymorphs and restored by replacement of cytophilic immunoglobulin. It was shown by staining polymorphs with FITC-conjugated anti-immunoglobulin sera that cytophilic immunoglobulin on the surface of polymorphs belonged to the IgG2 class of immunoglobulins. When 'neutral' polymorphs (from non-immunized ewes) were coated with IgG2 purified from the sera of infected ewes, they exhibited enhanced phagocytosis of staphylococci compared with 'neutral' polymorphs carying IgG2 from the sera of ewes given the killed vaccine.", "contents": "The effect of cytophilic IgG2 on phagocytosis by ovine polymorphonuclear leucocytes. Non-lactating, multiparous ewes were immunized either by subcutaneous infection with live Staphylococcus aureus (seventeen ewes) or by intramuscular injections of a killed S. aureus-oil adjuvant vaccine (seventeen ewes). Polymorphs which were subsequently collected from the mammary glands of the animals were used in in vitro phagocytosis assays against Pseudomonas sp. or S. aureus. There was no difference between polymorphs from the two groups of ewes in their ability to phagocytose Pseudomonas organisms. Polymorphs from the infected ewes showed significant phagocytic superiority over cells from ewes given the killed vaccine when S. aureus was the target organism. This phagocytic superiority could be abrogated by removal of cytophilic immunoglobulin from polymorphs and restored by replacement of cytophilic immunoglobulin. It was shown by staining polymorphs with FITC-conjugated anti-immunoglobulin sera that cytophilic immunoglobulin on the surface of polymorphs belonged to the IgG2 class of immunoglobulins. When 'neutral' polymorphs (from non-immunized ewes) were coated with IgG2 purified from the sera of infected ewes, they exhibited enhanced phagocytosis of staphylococci compared with 'neutral' polymorphs carying IgG2 from the sera of ewes given the killed vaccine.", "PMID": 955674}
{"id": "pubmed23n0032_1734", "title": "Secondary cytotoxic cell response to lymphocytic choriomeningitis virus. I. Kinetics of induction in vitro and yields of effector cells.", "content": "Secondary (memory) cell-mediated cytotoxic responses in lymphoid cells from CBA/H mice pre-primed with lymphocytic choriomeningitis virus (LCM) 5-7 weeks previously were induced by culturing these cells in vitro with syngeneic, infected peritoneal cells at 37 degrees for periods of up to 5 days. Cytotoxic effectors were assayed against LCM infected, H-2 compatible target cells in a 51Cr release assay. Response was greater with a higher ratio (1:10) of infected peritoneal cells:pre-primed cells than with lower ratios (e.g. 1:250). Separating responders from infected cells by a 450 mmum nucleopore membrane (coarse enough to allow passage of virus particles) still permitted induction of a secondary response whilst interposition of a 50 mmum nucleopore membrane (which apparently prevented transit of virus particles) virtually abolished the secondary response. Removal of phagocytic cells from responders prior to setting up memory cultures greatly reduced responders' capacity to be induced. Fixed, infected stimulators still induced strong secondary responses. Secondary response was maximal with spleen cells, peripheral blood lymphocytes, or pooled iliac and lumbar lymph node cells. Thymocytes responded less well, whilst mesenteric lymphoid cells and peritoneal cells gave minimal responses. Effector cells from memory cultures killed targets with single-hit kinetics and a rectilinear log effectors: log targets lysed relation held. Memory spleen cells developed increasing cytolytic activity from 2 to 5 days in culture. Memory-generated effectors were markedly potent by day 5, e.g. giving 70 per cent specific release at a killer:target ratio of 0-8:1. Peak DNA synthesis occurred on day 4. We conclude that memory effectors as a population differ in kinetics and potency from effectors obtained by primary viral challenge in the mouse.", "contents": "Secondary cytotoxic cell response to lymphocytic choriomeningitis virus. I. Kinetics of induction in vitro and yields of effector cells. Secondary (memory) cell-mediated cytotoxic responses in lymphoid cells from CBA/H mice pre-primed with lymphocytic choriomeningitis virus (LCM) 5-7 weeks previously were induced by culturing these cells in vitro with syngeneic, infected peritoneal cells at 37 degrees for periods of up to 5 days. Cytotoxic effectors were assayed against LCM infected, H-2 compatible target cells in a 51Cr release assay. Response was greater with a higher ratio (1:10) of infected peritoneal cells:pre-primed cells than with lower ratios (e.g. 1:250). Separating responders from infected cells by a 450 mmum nucleopore membrane (coarse enough to allow passage of virus particles) still permitted induction of a secondary response whilst interposition of a 50 mmum nucleopore membrane (which apparently prevented transit of virus particles) virtually abolished the secondary response. Removal of phagocytic cells from responders prior to setting up memory cultures greatly reduced responders' capacity to be induced. Fixed, infected stimulators still induced strong secondary responses. Secondary response was maximal with spleen cells, peripheral blood lymphocytes, or pooled iliac and lumbar lymph node cells. Thymocytes responded less well, whilst mesenteric lymphoid cells and peritoneal cells gave minimal responses. Effector cells from memory cultures killed targets with single-hit kinetics and a rectilinear log effectors: log targets lysed relation held. Memory spleen cells developed increasing cytolytic activity from 2 to 5 days in culture. Memory-generated effectors were markedly potent by day 5, e.g. giving 70 per cent specific release at a killer:target ratio of 0-8:1. Peak DNA synthesis occurred on day 4. We conclude that memory effectors as a population differ in kinetics and potency from effectors obtained by primary viral challenge in the mouse.", "PMID": 955675}
{"id": "pubmed23n0032_1735", "title": "Studies on the interaction of the Sophora japonica lectin and concanavalin A with erythrocytes and lymphocytes.", "content": "The agglutinating activity of lectins from the seeds of Sophora japonica and Canavalia ensiformis (concanavalin A) with human and murine erythrocytes and lymphocytes have been compared to one another and related to the mitogenic and immunosuppressive properties of these purified proteins. The S. japonica lectin, which demonstrates blood group specificity, is more active than concanavalin A with human erythrocytes, but has a much lower reactivity than concanavalin A with murine red blood cells. Ficin treatment of human erythrocytes results in an increase in agglutinability by both lectins as well as causing the appearance of S. japonica lectin receptors on type O cells. Treatment of murine reythrocytes with ficin alone or followed by beta-galactosidase causes the cells to be more reactive with concanavalin A. Beta-Galactosidase alone has no observable affect on the cells. In contrast, the agglutinability of cells by the S. japonica lectin increases after ficin treatment but is not affected by beta-galaetosidose treatment either after or in the absence of ficinization. Murine lymphocytes react with both lectins in a manner paralleling the agglutination patterns of murine erythrocytes. The S. japonica lectin appears to be devoid of mitogenic and immuno-suppressive activity, in contrast to concanavalin A which suppresses the T helper-dependent antibody response to sheep erythrocytes. These results are discussed in terms of the types of lectin receptors on lymphocytes related to agglutination, induction of blastogenesis and immuno-suppression.", "contents": "Studies on the interaction of the Sophora japonica lectin and concanavalin A with erythrocytes and lymphocytes. The agglutinating activity of lectins from the seeds of Sophora japonica and Canavalia ensiformis (concanavalin A) with human and murine erythrocytes and lymphocytes have been compared to one another and related to the mitogenic and immunosuppressive properties of these purified proteins. The S. japonica lectin, which demonstrates blood group specificity, is more active than concanavalin A with human erythrocytes, but has a much lower reactivity than concanavalin A with murine red blood cells. Ficin treatment of human erythrocytes results in an increase in agglutinability by both lectins as well as causing the appearance of S. japonica lectin receptors on type O cells. Treatment of murine reythrocytes with ficin alone or followed by beta-galactosidase causes the cells to be more reactive with concanavalin A. Beta-Galactosidase alone has no observable affect on the cells. In contrast, the agglutinability of cells by the S. japonica lectin increases after ficin treatment but is not affected by beta-galaetosidose treatment either after or in the absence of ficinization. Murine lymphocytes react with both lectins in a manner paralleling the agglutination patterns of murine erythrocytes. The S. japonica lectin appears to be devoid of mitogenic and immuno-suppressive activity, in contrast to concanavalin A which suppresses the T helper-dependent antibody response to sheep erythrocytes. These results are discussed in terms of the types of lectin receptors on lymphocytes related to agglutination, induction of blastogenesis and immuno-suppression.", "PMID": 955676}
{"id": "pubmed23n0032_1736", "title": "Cell division and deoxyribonucleic acid (DNA) synthesis in cultures of stimulated lymphocytes.", "content": "The responses of lymphocytes cultured with various stimulants were analysed with respect to DNA synthesis and cell division. Autoradiographic labelling with [3H]thymidine indicated that similar proportions of cells had incorporated this labelled precursor for DNA synthesis during both short and long periods of exposure to this specific precursor for DNA synthesis. Changes in labelling index (LI) after pulsing these cells with [3H]thymidine showed that exchange of labelled material, which could not be chased out with unlabelled thymidine, was responsible for the increases of LI seen. Failure to prevent this increase with excess unlabelled thymidine indicated that direct incorporation of [3H]thymidine did not account for this exchange. Using hydroxyurea and colcemid arrest to analyse cell cycle events in these cultures, it was shown that approximately 70 per cent of the responding cells in cultures of stimulated lymphocytes, while actively synthesizing DNA, were not in cell cycle for division. It was concluded that DNA turnover, involving synthesis and exchange of newly synthesized material, possibly DNA, was occurring in these cells.", "contents": "Cell division and deoxyribonucleic acid (DNA) synthesis in cultures of stimulated lymphocytes. The responses of lymphocytes cultured with various stimulants were analysed with respect to DNA synthesis and cell division. Autoradiographic labelling with [3H]thymidine indicated that similar proportions of cells had incorporated this labelled precursor for DNA synthesis during both short and long periods of exposure to this specific precursor for DNA synthesis. Changes in labelling index (LI) after pulsing these cells with [3H]thymidine showed that exchange of labelled material, which could not be chased out with unlabelled thymidine, was responsible for the increases of LI seen. Failure to prevent this increase with excess unlabelled thymidine indicated that direct incorporation of [3H]thymidine did not account for this exchange. Using hydroxyurea and colcemid arrest to analyse cell cycle events in these cultures, it was shown that approximately 70 per cent of the responding cells in cultures of stimulated lymphocytes, while actively synthesizing DNA, were not in cell cycle for division. It was concluded that DNA turnover, involving synthesis and exchange of newly synthesized material, possibly DNA, was occurring in these cells.", "PMID": 955677}
{"id": "pubmed23n0032_1737", "title": "Inhibitory effect of carrier and hapten preimmunization on delayed hypersensitivity to the carrier.", "content": "The different components of the immune response to hapten-carrier conjugates have been supposed to interact with each other. In order to elucidate the interrelationship between hapten and carrier moieties of the antigen molecule at the cellular level, the homologous carrier alone or the hapten conjugated to an heterologous carrier were injected before immunization with the hapten-carrier conjugate. Delayed hypersensitivity to the carrier could be depressed by pretreatment with the hapten or with the carrier depending on which time interval was chosen to elapse between the two injections. The results could be explained by antigenic competition, occurring at the cellular level, between hapten and carrier moieties of the antigen molecule.", "contents": "Inhibitory effect of carrier and hapten preimmunization on delayed hypersensitivity to the carrier. The different components of the immune response to hapten-carrier conjugates have been supposed to interact with each other. In order to elucidate the interrelationship between hapten and carrier moieties of the antigen molecule at the cellular level, the homologous carrier alone or the hapten conjugated to an heterologous carrier were injected before immunization with the hapten-carrier conjugate. Delayed hypersensitivity to the carrier could be depressed by pretreatment with the hapten or with the carrier depending on which time interval was chosen to elapse between the two injections. The results could be explained by antigenic competition, occurring at the cellular level, between hapten and carrier moieties of the antigen molecule.", "PMID": 955678}
{"id": "pubmed23n0032_1738", "title": "The mediation of tissue eosinophilia in hypersensitivity reactions. II. Separation of a delayed eosinophil chemotactic factor from macrophage chemotactic factors.", "content": "In anaphylactic cutaneous lesions induced by DNP-ascaris extract in the guinea-pig, the time-course of delayed tissue eosinophilia was found to parallel that of the macrophage reaction, reaching its peak in 24 h. Macrophages could be differentiated from lymphocytes by the numerous lysosomal granules which stained for acid phosphatase. Extracts from such skin lesions contained a delayed eosinophil chemotactic factor and two different macrophage chemotactic factors. Most of the delayed eosinophil chemotactic factor was separated from the two macrophage chemotactic factors by gel filtration on Sephadex G-100 and Sephadex G-200 in that order. The eosinophil chemotactic factor after re-chromatography on Sephadex G-I99 showed no or little chemotactic activity for macrophages.", "contents": "The mediation of tissue eosinophilia in hypersensitivity reactions. II. Separation of a delayed eosinophil chemotactic factor from macrophage chemotactic factors. In anaphylactic cutaneous lesions induced by DNP-ascaris extract in the guinea-pig, the time-course of delayed tissue eosinophilia was found to parallel that of the macrophage reaction, reaching its peak in 24 h. Macrophages could be differentiated from lymphocytes by the numerous lysosomal granules which stained for acid phosphatase. Extracts from such skin lesions contained a delayed eosinophil chemotactic factor and two different macrophage chemotactic factors. Most of the delayed eosinophil chemotactic factor was separated from the two macrophage chemotactic factors by gel filtration on Sephadex G-100 and Sephadex G-200 in that order. The eosinophil chemotactic factor after re-chromatography on Sephadex G-I99 showed no or little chemotactic activity for macrophages.", "PMID": 955679}
{"id": "pubmed23n0032_1739", "title": "Lectin-dependent neutrophil-mediated cytotoxicity. I. Characteristics.", "content": "Isolated normal human peripheral neutrophils became cytotoxic to chicken red blood cells (CRBC) in the presence of phytohaemagglutinin (PHA) and concanavalin A (Con A), a phenomenon which we have termed lectin-dependent neutrophilmediated cytotoxicity (LDNMC). Substantial cytotoxicity could be demonstrated by 1 h of incubation at 37 degrees. Isolated human peripheral lymphocytes were not cytotoxic to CRBC in the presence of these lectins, even after 18 h of incubation. Both PHA and Con A exhibited dose responses over a wide concentration range and displayed progressive, time-dependent cytotoxicity. Cytotoxicity for both PHA and Con A was greater at 37 degrees than at 22 degrees, and was undetectable at 4 degrees. CRBC as target cells were much more readily lysed than either sheep or human erythrocytes. Erythrophagocytosis did not appear to play a role.", "contents": "Lectin-dependent neutrophil-mediated cytotoxicity. I. Characteristics. Isolated normal human peripheral neutrophils became cytotoxic to chicken red blood cells (CRBC) in the presence of phytohaemagglutinin (PHA) and concanavalin A (Con A), a phenomenon which we have termed lectin-dependent neutrophilmediated cytotoxicity (LDNMC). Substantial cytotoxicity could be demonstrated by 1 h of incubation at 37 degrees. Isolated human peripheral lymphocytes were not cytotoxic to CRBC in the presence of these lectins, even after 18 h of incubation. Both PHA and Con A exhibited dose responses over a wide concentration range and displayed progressive, time-dependent cytotoxicity. Cytotoxicity for both PHA and Con A was greater at 37 degrees than at 22 degrees, and was undetectable at 4 degrees. CRBC as target cells were much more readily lysed than either sheep or human erythrocytes. Erythrophagocytosis did not appear to play a role.", "PMID": 955680}
{"id": "pubmed23n0032_1740", "title": "Lectin-dependent neutrophil-mediated cytotoxicity. II. Possible mechanisms.", "content": "The mechanisms whereby neutrophils become cytotoxic to chicken erythrocyte (CRBC) target cells were investigated in a system of lectin-dependent neutrophil-mediated cytotoxicity (LDNMC). Through the use of drugs and specific metabolic inhibitors, LDNMC was found to be dependent on energy supplied by anaerobic glycolysis and on other active metabolic functions of the neutrophil. 2-Iodoacetamide, 2-deoxy-D-glucose, di-isopropyl-fluorophosphate, colchicine, cytochalasin B, and dibutyryl cyclic AMP all caused dose-dependent inhibition of cytotoxicity, while inhibitors of protein and nucleic acid synthesis were without effect. Cell surface membrane-active agents, such as chloroquine, hydrocortisone and chlorpromazine inhibited cytotoxicity, while vitamin A caused enhancement. Lectins which agglutinated neutrophils, but not necessarily CRBC, such as phytohaemagglutinin (PHA-P), concanavalin A (Con A), soybean agglutinin (SBA), and Ricinus communis agglutinin (RCA), mediated cytotoxicity while lectins which did not cause agglutination, such as pokeweed mitogen (PWM), did not mediate cytotoxicity. Preincubation of neutrophils, but not CRBC with PHA-P, resulted in time-dependent enhancement of cytotoxicity, while pre-incubation with Con A yielded progressive inhibition of cytotoxicity. These studies suggest that lectin binding to the cell surface causes alterations of the membrane, that LDNMC requires cell to cell surface contact, and that cytotoxicity depends on active metabolic processes.", "contents": "Lectin-dependent neutrophil-mediated cytotoxicity. II. Possible mechanisms. The mechanisms whereby neutrophils become cytotoxic to chicken erythrocyte (CRBC) target cells were investigated in a system of lectin-dependent neutrophil-mediated cytotoxicity (LDNMC). Through the use of drugs and specific metabolic inhibitors, LDNMC was found to be dependent on energy supplied by anaerobic glycolysis and on other active metabolic functions of the neutrophil. 2-Iodoacetamide, 2-deoxy-D-glucose, di-isopropyl-fluorophosphate, colchicine, cytochalasin B, and dibutyryl cyclic AMP all caused dose-dependent inhibition of cytotoxicity, while inhibitors of protein and nucleic acid synthesis were without effect. Cell surface membrane-active agents, such as chloroquine, hydrocortisone and chlorpromazine inhibited cytotoxicity, while vitamin A caused enhancement. Lectins which agglutinated neutrophils, but not necessarily CRBC, such as phytohaemagglutinin (PHA-P), concanavalin A (Con A), soybean agglutinin (SBA), and Ricinus communis agglutinin (RCA), mediated cytotoxicity while lectins which did not cause agglutination, such as pokeweed mitogen (PWM), did not mediate cytotoxicity. Preincubation of neutrophils, but not CRBC with PHA-P, resulted in time-dependent enhancement of cytotoxicity, while pre-incubation with Con A yielded progressive inhibition of cytotoxicity. These studies suggest that lectin binding to the cell surface causes alterations of the membrane, that LDNMC requires cell to cell surface contact, and that cytotoxicity depends on active metabolic processes.", "PMID": 955681}
{"id": "pubmed23n0032_1741", "title": "Miliary mycoplasmal pneumonia: a report of six cases.", "content": "Six cases of miliary pneumonia are reported, in which the marked increase in antibody titers showed that Mycoplasma pneumoniae was the probable cause. The clinical picture of these patients showed an acute and severe onset, with the appearance of diffuse alveolitis. The outcome of the disease depended on the time of initiation of treatment in relationship to the onset of symptoms. One case was seen to progress to fibrosing alveolitis with persistent impairment of diffusion. This observation suggests the possibility that some cases of pulmonary fibrosis of unknown aetiology may be due to previous mycoplasmal infection.", "contents": "Miliary mycoplasmal pneumonia: a report of six cases. Six cases of miliary pneumonia are reported, in which the marked increase in antibody titers showed that Mycoplasma pneumoniae was the probable cause. The clinical picture of these patients showed an acute and severe onset, with the appearance of diffuse alveolitis. The outcome of the disease depended on the time of initiation of treatment in relationship to the onset of symptoms. One case was seen to progress to fibrosing alveolitis with persistent impairment of diffusion. This observation suggests the possibility that some cases of pulmonary fibrosis of unknown aetiology may be due to previous mycoplasmal infection.", "PMID": 955695}
{"id": "pubmed23n0032_1742", "title": "Effect of Mycoplasma pheumoniae infection on cell-mediated immunity.", "content": "The effect of Mycoplasma pneumoniae infection on cell-mediated immunity was examined by two methods: the first method was the tuberculin skin test and the other was the measurement of the in vitro lymphocyte stimulation response to purified protein derivative (PPD) of tuberculin. Twenty-nine patients out of a total of 47 patients with a lower respiratory tract illness caused by M. pneumoniae had a negative tuberculin skin test when first tested. Twenty-three out of 26 patients with a negative tuberculin reaction in the early phase of the illness had a positive skin test when they were retested several weeks or months after the illness. The lymphocyte stimulation response to PPD was examined in 13 patients. In eight of these cases the lymphocyte response to PPD was significantly lower during illness than after recovery. Six control subjects showed no significant difference in their lymphocyte responsiveness to PPD when examined on two different occasions. These studies show that M.pneumoniae infection causes transient depression of cell-mediated immunity.", "contents": "Effect of Mycoplasma pheumoniae infection on cell-mediated immunity. The effect of Mycoplasma pneumoniae infection on cell-mediated immunity was examined by two methods: the first method was the tuberculin skin test and the other was the measurement of the in vitro lymphocyte stimulation response to purified protein derivative (PPD) of tuberculin. Twenty-nine patients out of a total of 47 patients with a lower respiratory tract illness caused by M. pneumoniae had a negative tuberculin skin test when first tested. Twenty-three out of 26 patients with a negative tuberculin reaction in the early phase of the illness had a positive skin test when they were retested several weeks or months after the illness. The lymphocyte stimulation response to PPD was examined in 13 patients. In eight of these cases the lymphocyte response to PPD was significantly lower during illness than after recovery. Six control subjects showed no significant difference in their lymphocyte responsiveness to PPD when examined on two different occasions. These studies show that M.pneumoniae infection causes transient depression of cell-mediated immunity.", "PMID": 955696}
{"id": "pubmed23n0032_1743", "title": "Lymphocyte transformation studies in Mycoplasma pneumoniae infections.", "content": "The in vitro transformation of lymphocytes stimulated by a Mycoplasma pneumoniae preparation was measured by the uptake of 14C-thymidine. The lymphocytes from five patients with M.pneumoniae pneumonia showed a high degree of responsiveness when they were compared to the lymphocytes taken from eleven healthy control subjects who lacked M.pneumoniae antibodies. Another four patients with an acute affection of the central nervous system and serological evidence of an actual or recent M.pneumoniae infection had a lymphocyte response within the same range as that of the controls. The transformation of lymphocytes was studied at intervals for seven months after the onset of the illness in one of the patients with pneumonia. These studies showed an increasing response to a small dose of mycoplasma antigen. Lymphocyte transformation induced by other microbial antigens was studied in three pneumonia patients during and after convalescence. The first responses were low and increased more steeply than the response to M.pneumoniae. The later responses to the mycoplasmal and to the other microbial antigens increased in parallel. The usefulness of incorporating other microbial antigens in the evaluation of the patient's immune response to a relevant antigen in this type of experiment is discussed.", "contents": "Lymphocyte transformation studies in Mycoplasma pneumoniae infections. The in vitro transformation of lymphocytes stimulated by a Mycoplasma pneumoniae preparation was measured by the uptake of 14C-thymidine. The lymphocytes from five patients with M.pneumoniae pneumonia showed a high degree of responsiveness when they were compared to the lymphocytes taken from eleven healthy control subjects who lacked M.pneumoniae antibodies. Another four patients with an acute affection of the central nervous system and serological evidence of an actual or recent M.pneumoniae infection had a lymphocyte response within the same range as that of the controls. The transformation of lymphocytes was studied at intervals for seven months after the onset of the illness in one of the patients with pneumonia. These studies showed an increasing response to a small dose of mycoplasma antigen. Lymphocyte transformation induced by other microbial antigens was studied in three pneumonia patients during and after convalescence. The first responses were low and increased more steeply than the response to M.pneumoniae. The later responses to the mycoplasmal and to the other microbial antigens increased in parallel. The usefulness of incorporating other microbial antigens in the evaluation of the patient's immune response to a relevant antigen in this type of experiment is discussed.", "PMID": 955697}
{"id": "pubmed23n0032_1744", "title": "Epidemiology of Mycoplasma pneumoniae infection in the United Kingdom: an analysis of reports to the Public Health Laboratory Service of England and Wales.", "content": "Infection with Mycoplasma pneumoniae appears to follow a cyclic pattern in the United Kindom, with periods of increased prevalence lasting about two years. Outbreaks occurred in 1971-1972 and 1974-75, and probably also in 1967-68. Infection confirmed by laboratory tests appeared to be commonest in older children, especially those aged 5-14, and least common in adults over 65 years of age. Lower respiratory tract infection was the clinical feature most frequently reported.", "contents": "Epidemiology of Mycoplasma pneumoniae infection in the United Kingdom: an analysis of reports to the Public Health Laboratory Service of England and Wales. Infection with Mycoplasma pneumoniae appears to follow a cyclic pattern in the United Kindom, with periods of increased prevalence lasting about two years. Outbreaks occurred in 1971-1972 and 1974-75, and probably also in 1967-68. Infection confirmed by laboratory tests appeared to be commonest in older children, especially those aged 5-14, and least common in adults over 65 years of age. Lower respiratory tract infection was the clinical feature most frequently reported.", "PMID": 955698}
{"id": "pubmed23n0032_1745", "title": "The laboratory diagnosis and epidemiology of Mycoplasma pneumoniae in Switzerland.", "content": "The analysis of approximately 60,000 serum samples collected from patients with different clinical symptoms between 1965 and 1975 in Switzerland yielded positive reactions indicating a recent infection with Mycoplasma pneumoniae in 2-30% during this period. Among 1,555 patients with clinical symptoms of an acute respiratory infection, 359 patients or 23% had antibody titers indicating a recent infection with M.pneumoniae. On the basis of these results it is concluded that M.pneumoniae is the most frequent cause of acute infections of the respiratory tract.", "contents": "The laboratory diagnosis and epidemiology of Mycoplasma pneumoniae in Switzerland. The analysis of approximately 60,000 serum samples collected from patients with different clinical symptoms between 1965 and 1975 in Switzerland yielded positive reactions indicating a recent infection with Mycoplasma pneumoniae in 2-30% during this period. Among 1,555 patients with clinical symptoms of an acute respiratory infection, 359 patients or 23% had antibody titers indicating a recent infection with M.pneumoniae. On the basis of these results it is concluded that M.pneumoniae is the most frequent cause of acute infections of the respiratory tract.", "PMID": 955699}
{"id": "pubmed23n0032_1746", "title": "The frequency of Mycoplasma pneumoniae antibodies in a Calabrian population.", "content": "The epidemiological survey was carried out in a Calabrian population in order to ascertain the incidence of antibodies to Mycoplasma pneumoniae. In the past it has been felt that this infection does not occur in Southern Italy. We have found an incidence in our survey equal to that of the other countries. It was impossible to tell when these infections had occurred and the possibility of a continuing survey is discussed.", "contents": "The frequency of Mycoplasma pneumoniae antibodies in a Calabrian population. The epidemiological survey was carried out in a Calabrian population in order to ascertain the incidence of antibodies to Mycoplasma pneumoniae. In the past it has been felt that this infection does not occur in Southern Italy. We have found an incidence in our survey equal to that of the other countries. It was impossible to tell when these infections had occurred and the possibility of a continuing survey is discussed.", "PMID": 955700}
{"id": "pubmed23n0032_1747", "title": "A general practice survey of the incidence of Mycoplasma pneumoniae infections.", "content": "An epidemiological survey was carried out at two centres in the north of England (Liverpool and Sunderland). One hundred and twenty patients were included in this survey to determine the relative incidence of Mycoplasma pneumoniae infections in patients who present to their physician with an acute lower respiratory tract infection. Data were available at the end of the survey in 115 patients. Only one patient had a positive test for M.pneumoniae. There were nine patients who had a positive antibody test for either influenza or para-influenza. Four patients had a positive test for respiratory syncytial viruses. This survey points out the problems in the clinical diagnosis of respiratory infections in general practice.", "contents": "A general practice survey of the incidence of Mycoplasma pneumoniae infections. An epidemiological survey was carried out at two centres in the north of England (Liverpool and Sunderland). One hundred and twenty patients were included in this survey to determine the relative incidence of Mycoplasma pneumoniae infections in patients who present to their physician with an acute lower respiratory tract infection. Data were available at the end of the survey in 115 patients. Only one patient had a positive test for M.pneumoniae. There were nine patients who had a positive antibody test for either influenza or para-influenza. Four patients had a positive test for respiratory syncytial viruses. This survey points out the problems in the clinical diagnosis of respiratory infections in general practice.", "PMID": 955701}
{"id": "pubmed23n0032_1748", "title": "The treatment of primary atypical pneumonia with intravenous doxycycline: a report of clinical observations.", "content": "Seventeen cases of primary atypical pneumonia which were probably caused by Mycoplasma pneumoniae were studied as in-patients in hospital. All of these patients were treated with intravenous doxycycline and responded quickly to therapy. It has been shown that approximately 30-50% of the cases with primary atypical pneumonia are caused by M. pneumoniae. The remainder of the cases are caused by either viruses or possibly rickettsiae.", "contents": "The treatment of primary atypical pneumonia with intravenous doxycycline: a report of clinical observations. Seventeen cases of primary atypical pneumonia which were probably caused by Mycoplasma pneumoniae were studied as in-patients in hospital. All of these patients were treated with intravenous doxycycline and responded quickly to therapy. It has been shown that approximately 30-50% of the cases with primary atypical pneumonia are caused by M. pneumoniae. The remainder of the cases are caused by either viruses or possibly rickettsiae.", "PMID": 955702}
{"id": "pubmed23n0032_1749", "title": "Hospital treatment and diagnosis of Mycoplasma pneumoniae pneumonia.", "content": "A study was started in 1972 in order to determine the true etiology of patients who presented with obscure pulmonary infiltrates. Patients who were included in this study had sera drawn on two occasions. Complete testing was performed for the various viruses and also for Mycoplasma pneumoniae antibodies. The study continued through 1975 and a total of 159 patients were included. Of these 159 patients, 28 had positive complement fixation tests for M. pneumoniae antibodies. The clinical profile and treatment course is outlined and discussed.", "contents": "Hospital treatment and diagnosis of Mycoplasma pneumoniae pneumonia. A study was started in 1972 in order to determine the true etiology of patients who presented with obscure pulmonary infiltrates. Patients who were included in this study had sera drawn on two occasions. Complete testing was performed for the various viruses and also for Mycoplasma pneumoniae antibodies. The study continued through 1975 and a total of 159 patients were included. Of these 159 patients, 28 had positive complement fixation tests for M. pneumoniae antibodies. The clinical profile and treatment course is outlined and discussed.", "PMID": 955703}
{"id": "pubmed23n0032_1750", "title": "Mycoplasma pneumoniae as a cause of pneumonia, lung abscess and pleural effusion: a case report.", "content": "A case report of a 26 year-old asthmatic male is outlined in order to show the clinical features and unusual development pattern of pulmonary disorders and pleural effusion with a Mycoplasma pneumoniae pneumonia. A literature review is included in order to put this unusual case into perspective.", "contents": "Mycoplasma pneumoniae as a cause of pneumonia, lung abscess and pleural effusion: a case report. A case report of a 26 year-old asthmatic male is outlined in order to show the clinical features and unusual development pattern of pulmonary disorders and pleural effusion with a Mycoplasma pneumoniae pneumonia. A literature review is included in order to put this unusual case into perspective.", "PMID": 955704}
{"id": "pubmed23n0032_1751", "title": "Myocarditis and severe bilateral bronchopneumonia caused by Mycoplasma pneumoniae.", "content": "A man with severe bilateral bronchopneumonia and a right lobar consolidation caused by Mycoplasma pneumoniae, had a concurrent myocarditis. The possible aetiology of the patchy pulmonary opacities is discussed. A literature review of cases of myocarditis associated with M.pneumoniae is presented.", "contents": "Myocarditis and severe bilateral bronchopneumonia caused by Mycoplasma pneumoniae. A man with severe bilateral bronchopneumonia and a right lobar consolidation caused by Mycoplasma pneumoniae, had a concurrent myocarditis. The possible aetiology of the patchy pulmonary opacities is discussed. A literature review of cases of myocarditis associated with M.pneumoniae is presented.", "PMID": 955705}
{"id": "pubmed23n0032_1752", "title": "The results of pulmonary function tests in patients infected with Mycoplasma pneumoniae.", "content": "A comparative study was undertaken in order to ascertain the effects of a Mycoplasma pneumoniae infection on pulmonary function. It appears that the literature has many articles devoted to the clinical symptomatology of this type of infection but there is very little information available on the abnormalities which occur in the lungs with this infection. The patients who were used as a comparative model had lung lesions which gave a similar radiographic appearance. The significant finding was that an obstructive syndrome with increased airway resistance was noted in the M.pneumoniae pneumonia patients.", "contents": "The results of pulmonary function tests in patients infected with Mycoplasma pneumoniae. A comparative study was undertaken in order to ascertain the effects of a Mycoplasma pneumoniae infection on pulmonary function. It appears that the literature has many articles devoted to the clinical symptomatology of this type of infection but there is very little information available on the abnormalities which occur in the lungs with this infection. The patients who were used as a comparative model had lung lesions which gave a similar radiographic appearance. The significant finding was that an obstructive syndrome with increased airway resistance was noted in the M.pneumoniae pneumonia patients.", "PMID": 955706}
{"id": "pubmed23n0032_1753", "title": "Pulmonary function in children with Mycoplasma pneumoniae pneumonia.", "content": "Nine children between the ages of seven to 12 were studied. All of these children had an acute unilateral pneumonia caused by Mycoplasma pneumoniae. Regional pulmonary function studies were performed with the aid of an Xe133 radio-spirometry. It was shown with this technique that the ventilation of the infected part was more reduced than the perfusion during the acute stage. Fourteen to 22 days after the onset of the disease three children had normal chest X-rays but abnormalities of the pulmonary function tests. In one child this abnormal function persisted at the third follow-up some months later. By the use of regional pulmonary function studies subtle abnormalities can be observed which would normally be overlooked when examining the pulmonary function of both lungs.", "contents": "Pulmonary function in children with Mycoplasma pneumoniae pneumonia. Nine children between the ages of seven to 12 were studied. All of these children had an acute unilateral pneumonia caused by Mycoplasma pneumoniae. Regional pulmonary function studies were performed with the aid of an Xe133 radio-spirometry. It was shown with this technique that the ventilation of the infected part was more reduced than the perfusion during the acute stage. Fourteen to 22 days after the onset of the disease three children had normal chest X-rays but abnormalities of the pulmonary function tests. In one child this abnormal function persisted at the third follow-up some months later. By the use of regional pulmonary function studies subtle abnormalities can be observed which would normally be overlooked when examining the pulmonary function of both lungs.", "PMID": 955707}
{"id": "pubmed23n0032_1754", "title": "Patterns of personality developement in middle-aged women: a longitudinal study.", "content": "Personality development was examined in two groups of women studied since adolescence who were judged psychologically healthy at age fifty: 1) Independants, whose health improved from forty to fifty, were ambitious and intellectual. 2) Traditionals, healthy at both ages, were gregarious and nurturant. Traditionals showed steady personality growth since adolescence. Independents were constricted at age forty but recovered by fifty. These patterns are compared in terms of the fit between personality and sex role. Traditional personalitites fit conventional feminine roles, accounting for their health throughout the middle years. Independents improved when disengaging from mothering freed them to develop their more assertive skills.", "contents": "Patterns of personality developement in middle-aged women: a longitudinal study. Personality development was examined in two groups of women studied since adolescence who were judged psychologically healthy at age fifty: 1) Independants, whose health improved from forty to fifty, were ambitious and intellectual. 2) Traditionals, healthy at both ages, were gregarious and nurturant. Traditionals showed steady personality growth since adolescence. Independents were constricted at age forty but recovered by fifty. These patterns are compared in terms of the fit between personality and sex role. Traditional personalitites fit conventional feminine roles, accounting for their health throughout the middle years. Independents improved when disengaging from mothering freed them to develop their more assertive skills.", "PMID": 955717}
{"id": "pubmed23n0032_1755", "title": "Personality organizations of well-functioning younger people and older adults.", "content": "Person clustering of Q sort items for two longitudinal samples, studied at four periods from adolescence to middle adulthood, were generated for the sexes separately. After correlations between the resulting sixty-eight clusters showed that common personality organizations existed across time, sex, and samples, a second order clustering of the first sixty-eight was done which reduced the number of clusters to four. The first cluster represented a well-functioning organization, and comparisons were made of its hierarchial ordering of attributes between the longitudinal samples and a geriatric sample. Although both groups were dependable and productive, they diverged in the importance of intimate interpersonal relations and in their self concepts. The older people were more tender and intimate, but conserving of their own integrity, while the younger groups were more assertive and cognitively invested, as they must be to deal with their different life situations.", "contents": "Personality organizations of well-functioning younger people and older adults. Person clustering of Q sort items for two longitudinal samples, studied at four periods from adolescence to middle adulthood, were generated for the sexes separately. After correlations between the resulting sixty-eight clusters showed that common personality organizations existed across time, sex, and samples, a second order clustering of the first sixty-eight was done which reduced the number of clusters to four. The first cluster represented a well-functioning organization, and comparisons were made of its hierarchial ordering of attributes between the longitudinal samples and a geriatric sample. Although both groups were dependable and productive, they diverged in the importance of intimate interpersonal relations and in their self concepts. The older people were more tender and intimate, but conserving of their own integrity, while the younger groups were more assertive and cognitively invested, as they must be to deal with their different life situations.", "PMID": 955718}
{"id": "pubmed23n0032_1756", "title": "Midlife marriage: sex differences in evaluation and perspectives.", "content": "Perceptions of marital relations are examined in a white middle and lower-middle class sample representing three life stages: newlyweds, middle-aged parents facing the empty nest, and persons about to retire. Descriptions of spouses are analyed in terms of positive/negative evaluations and the respective importance given to role and interpersonal components of the marital relationship. Middle aged respondents were shown to place greater emphasis on role performance than did the remaining groups, and middle-aged women gave the least positive evaluations of marriage. Midlife marital satisfaction is examined in relationship to affect toward children, socioeconomic status, value orientations, and life satisfaction.", "contents": "Midlife marriage: sex differences in evaluation and perspectives. Perceptions of marital relations are examined in a white middle and lower-middle class sample representing three life stages: newlyweds, middle-aged parents facing the empty nest, and persons about to retire. Descriptions of spouses are analyed in terms of positive/negative evaluations and the respective importance given to role and interpersonal components of the marital relationship. Middle aged respondents were shown to place greater emphasis on role performance than did the remaining groups, and middle-aged women gave the least positive evaluations of marriage. Midlife marital satisfaction is examined in relationship to affect toward children, socioeconomic status, value orientations, and life satisfaction.", "PMID": 955719}
{"id": "pubmed23n0032_1757", "title": "Middle age: some early returns-a commentary.", "content": "Four papers, differing in research design and using both longitudinal and cross-sectional data, came to a number of convergent conclusions: there was little evidence for a midlife crisis; stability was most prominent during the forty-to fifty-year decade; sex differences were striking, this period being most stressful for women; stable personality characteristics show interactions with role-stage expectations. Caveats as to generalizability of conclusions and lack of cohort comparison were stated. Certain theoretical issues were discussed related to the meaning of \"middle age,\" developmental dynamics, and sources of stability and change.", "contents": "Middle age: some early returns-a commentary. Four papers, differing in research design and using both longitudinal and cross-sectional data, came to a number of convergent conclusions: there was little evidence for a midlife crisis; stability was most prominent during the forty-to fifty-year decade; sex differences were striking, this period being most stressful for women; stable personality characteristics show interactions with role-stage expectations. Caveats as to generalizability of conclusions and lack of cohort comparison were stated. Certain theoretical issues were discussed related to the meaning of \"middle age,\" developmental dynamics, and sources of stability and change.", "PMID": 955720}
{"id": "pubmed23n0032_1758", "title": "Masculinity/femininity of the generations: as self-reported and as stereotypically appraised.", "content": "Three hundred seventeen adults divided among the younger adult, middle aged, and old generations were asked (a) to judge which of the three generations was most and least masculine/feminine in overall personality style, masculine/feminine in interests and possession of gender skills, and pressured by society to do masculine/feminine sorts of things; and (b) to rate self along each of these dimensions on a Likert scale. Self-reports and beliefs about the generations did not vary as a function of membership in a generation. Young adults and middle aged were belived to be the most feminine and middle aged the most masculine in overall personality style. The middle aged were judged as possessed of the most feminine and masculine interests and the most skilled in feminine endeavors. Middle aged and young adults were judged equally possessed of masculine skills. Social pressure to do masculine and feminine activities was seen as falling equally heavily on younger adults and middle aged. The old were judged as next-most possessed of feminine skills while judged least along the other dimensions.", "contents": "Masculinity/femininity of the generations: as self-reported and as stereotypically appraised. Three hundred seventeen adults divided among the younger adult, middle aged, and old generations were asked (a) to judge which of the three generations was most and least masculine/feminine in overall personality style, masculine/feminine in interests and possession of gender skills, and pressured by society to do masculine/feminine sorts of things; and (b) to rate self along each of these dimensions on a Likert scale. Self-reports and beliefs about the generations did not vary as a function of membership in a generation. Young adults and middle aged were belived to be the most feminine and middle aged the most masculine in overall personality style. The middle aged were judged as possessed of the most feminine and masculine interests and the most skilled in feminine endeavors. Middle aged and young adults were judged equally possessed of masculine skills. Social pressure to do masculine and feminine activities was seen as falling equally heavily on younger adults and middle aged. The old were judged as next-most possessed of feminine skills while judged least along the other dimensions.", "PMID": 955721}
{"id": "pubmed23n0032_1759", "title": "Identity and crisis in middle aged men.", "content": "The authors selectively review disparate literature bearing on the issue of the male mid-life transition. There is considerable evidence that the entrance into middle age is associated with stress and often maladaptive patterns of reaction. There is little available information, however, on the specific nature of the stressors, their differential impact, or the factors associated with the individual's attempted style of adaptation to them. Through the process of exploring relevant findings from sociology, social and clinical psychology, and popular fiction we attempt to specify a working model of the experience of entering middle age. The authors see this model as being of heuristic value in that it specifies a number of dimensions to be operationalized which may, together, provide a more holistic view of the changes in the self-system undergone by the middle aged male.", "contents": "Identity and crisis in middle aged men. The authors selectively review disparate literature bearing on the issue of the male mid-life transition. There is considerable evidence that the entrance into middle age is associated with stress and often maladaptive patterns of reaction. There is little available information, however, on the specific nature of the stressors, their differential impact, or the factors associated with the individual's attempted style of adaptation to them. Through the process of exploring relevant findings from sociology, social and clinical psychology, and popular fiction we attempt to specify a working model of the experience of entering middle age. The authors see this model as being of heuristic value in that it specifies a number of dimensions to be operationalized which may, together, provide a more holistic view of the changes in the self-system undergone by the middle aged male.", "PMID": 955722}
{"id": "pubmed23n0032_1760", "title": "Are aged blacks who manifest differences in community security also different in coping reactions?", "content": "Seventy-two aged Blacks, equally divided between those residing in a high-rise public housing project and those living in the community awaiting admission were interviewed to test the theory that differences in community security would predict differences in coping reactions. Data on perceived community security and reported medical, social and mental coping reactions were colleted, using a simply and directly worded, precoded, stimulus-response instrument developed by the author. Respondents were assigned to either a high or to a low community security group by two methods: first, they were assigned a place of residence; next, they were assigned according to their obtained community security score. Results in general supported the theory. Of the two empirical indicators of community security, however, larger differences in coping reaction scores were found in the high scoring and low scoring community security groups than in the high-rise and community groups.", "contents": "Are aged blacks who manifest differences in community security also different in coping reactions? Seventy-two aged Blacks, equally divided between those residing in a high-rise public housing project and those living in the community awaiting admission were interviewed to test the theory that differences in community security would predict differences in coping reactions. Data on perceived community security and reported medical, social and mental coping reactions were colleted, using a simply and directly worded, precoded, stimulus-response instrument developed by the author. Respondents were assigned to either a high or to a low community security group by two methods: first, they were assigned a place of residence; next, they were assigned according to their obtained community security score. Results in general supported the theory. Of the two empirical indicators of community security, however, larger differences in coping reaction scores were found in the high scoring and low scoring community security groups than in the high-rise and community groups.", "PMID": 955723}
{"id": "pubmed23n0032_1761", "title": "Glimpses into the social world of middle age.", "content": "This paper describes briefly the two cohorts recently followed up in the long term longitudinal research program at the Institute of Human Development at Berkeley, and notes the characteristics of those members who remain under study. It then presents a few findings on the occupational carreers and work orientations of both men and women, notes certain personality correlates of career patterns in the middle years, and briefly touches on marital satisfaction as related to work status.", "contents": "Glimpses into the social world of middle age. This paper describes briefly the two cohorts recently followed up in the long term longitudinal research program at the Institute of Human Development at Berkeley, and notes the characteristics of those members who remain under study. It then presents a few findings on the occupational carreers and work orientations of both men and women, notes certain personality correlates of career patterns in the middle years, and briefly touches on marital satisfaction as related to work status.", "PMID": 955725}
{"id": "pubmed23n0032_1762", "title": "Impaired general cell-mediated immune functions in vivo and in vitro in patients with nasopharyngeal carcinoma.", "content": "General cell-mediated immune (CMI) functions in NPC patients were investigated by the in vivo Mantoux and in vitro lymphocyte response to PHA assays. Thirty-eight (50%) of 76 untreated NPC patients were hyporesponsive in the Mantoux assay compared to 27 (25%) of 110 control patients. Forty-three (65.2%) of 66 untreated NPC patients also showed lymphocyte hyporesponsiveness to PHA compared to 15 (15.5%) of 97 control patients. Combined deficiencies were observed in 35 (42.2%) of 83 NPC patients compared to only 2 (3.3%) of 61 control patients. No difference in the frequency of immunodeficiency was observed between \"early\" and \"late\" disease patients. CMI functions of treated \"remission\" NPC patients were found to be impaired to the same extent as those of untreated NPC patients.", "contents": "Impaired general cell-mediated immune functions in vivo and in vitro in patients with nasopharyngeal carcinoma. General cell-mediated immune (CMI) functions in NPC patients were investigated by the in vivo Mantoux and in vitro lymphocyte response to PHA assays. Thirty-eight (50%) of 76 untreated NPC patients were hyporesponsive in the Mantoux assay compared to 27 (25%) of 110 control patients. Forty-three (65.2%) of 66 untreated NPC patients also showed lymphocyte hyporesponsiveness to PHA compared to 15 (15.5%) of 97 control patients. Combined deficiencies were observed in 35 (42.2%) of 83 NPC patients compared to only 2 (3.3%) of 61 control patients. No difference in the frequency of immunodeficiency was observed between \"early\" and \"late\" disease patients. CMI functions of treated \"remission\" NPC patients were found to be impaired to the same extent as those of untreated NPC patients.", "PMID": 955740}
{"id": "pubmed23n0032_1763", "title": "Design of experiments using the microcytotoxicity assay.", "content": "Examination of data from the microcytotoxicity assay revealed that the number of surviving target cells varies systematically both between microtest plates and within plates. If the layout of treatments on the plate is not planned carefully, these effects decreased precision and introduce bias. It is recommended that treatments which are to be compared be placed close together on the plate; for example, a plate with 96 wells might be considered as 24 blocks of 4 wells, with the treatments placed within these blocks. Data can be analyzed by means of paired t-tests or randomized block analysis of variance. These design changes remove the effect of systematic variation and increase the precision of the assay.", "contents": "Design of experiments using the microcytotoxicity assay. Examination of data from the microcytotoxicity assay revealed that the number of surviving target cells varies systematically both between microtest plates and within plates. If the layout of treatments on the plate is not planned carefully, these effects decreased precision and introduce bias. It is recommended that treatments which are to be compared be placed close together on the plate; for example, a plate with 96 wells might be considered as 24 blocks of 4 wells, with the treatments placed within these blocks. Data can be analyzed by means of paired t-tests or randomized block analysis of variance. These design changes remove the effect of systematic variation and increase the precision of the assay.", "PMID": 955741}
{"id": "pubmed23n0032_1764", "title": "Production of tumor-specific inducer of cellular cytotoxicity by lethally irradiated mice.", "content": "Antisera taken 1 or 2 days after inoculation of BALB/c mice with transplantable sarcoma cells or Moloney sarcoma virus (MSV) induce tumor-specific cell-dependent cytotoxicity in vitro. In the present experiments, lethally irradiated (\"immunosuppressed\") mice were tested for the early appearance of the serum factor responsible for this anti-serum-dependent cell-mediated cytotoxicity (E-ADC). BALB/c mice were infected with MSV, syngeneic sarcoma cells or sheep red blood cells 24 h following irradiation with 900 R. Sera were obtained from MSV and tumor cell recipients 48 or 72 h later and tested for E-ADC activity. Spleen cells from SRBC recipients were tested at day 4 or 5 for ability to form direct plaques in a modified Jerne plague assay. Although the anti-SRBC response was obliterated in the irradiated mice, the E-ADC response appeared to be unimpaired. These studies indicate that newly synthesized immunoglobulin is not required for the formation of the E-ADC factor.", "contents": "Production of tumor-specific inducer of cellular cytotoxicity by lethally irradiated mice. Antisera taken 1 or 2 days after inoculation of BALB/c mice with transplantable sarcoma cells or Moloney sarcoma virus (MSV) induce tumor-specific cell-dependent cytotoxicity in vitro. In the present experiments, lethally irradiated (\"immunosuppressed\") mice were tested for the early appearance of the serum factor responsible for this anti-serum-dependent cell-mediated cytotoxicity (E-ADC). BALB/c mice were infected with MSV, syngeneic sarcoma cells or sheep red blood cells 24 h following irradiation with 900 R. Sera were obtained from MSV and tumor cell recipients 48 or 72 h later and tested for E-ADC activity. Spleen cells from SRBC recipients were tested at day 4 or 5 for ability to form direct plaques in a modified Jerne plague assay. Although the anti-SRBC response was obliterated in the irradiated mice, the E-ADC response appeared to be unimpaired. These studies indicate that newly synthesized immunoglobulin is not required for the formation of the E-ADC factor.", "PMID": 955742}
{"id": "pubmed23n0032_1765", "title": "The distribution and fate of blood-borne 125IUdR-labelled tumour cells in immune syngeneic rats.", "content": "Radiolabelled sarcoma cells injected into the tail veins of normal rats were held up almost exclusively in the lung, and were not observed to pass through into the systemic circulation. Intramuscularly injected tumour cells were retained at the site of injection. Radioactivity was lost from both sites though more rapidly from the lung than from muscular tissue and was probably the result of tumour-cell death. Alveolar macrophages did not take part in the destruction of tumour cells in the lung. There was an increased rate of radiolabel loss from the lungs of hyperimmune, post-excision and tumour-bearing rats, as compared with normal rats. The destruction was immunologically specific; it was detected earlier, was more comprehensive in the hyperimmune and post-excision animals than in tumour-bearing animals, and correlated with the ability of the hyperimmune and post-excision animals to reject larger numbers of intravenous unlabelled tumour cells, than the tumour-bearing rats.", "contents": "The distribution and fate of blood-borne 125IUdR-labelled tumour cells in immune syngeneic rats. Radiolabelled sarcoma cells injected into the tail veins of normal rats were held up almost exclusively in the lung, and were not observed to pass through into the systemic circulation. Intramuscularly injected tumour cells were retained at the site of injection. Radioactivity was lost from both sites though more rapidly from the lung than from muscular tissue and was probably the result of tumour-cell death. Alveolar macrophages did not take part in the destruction of tumour cells in the lung. There was an increased rate of radiolabel loss from the lungs of hyperimmune, post-excision and tumour-bearing rats, as compared with normal rats. The destruction was immunologically specific; it was detected earlier, was more comprehensive in the hyperimmune and post-excision animals than in tumour-bearing animals, and correlated with the ability of the hyperimmune and post-excision animals to reject larger numbers of intravenous unlabelled tumour cells, than the tumour-bearing rats.", "PMID": 955743}
{"id": "pubmed23n0032_1766", "title": "Some international differences in treatment and survival in breast cancer.", "content": "In a recent study, 5-year survival rates for breast cancer patients in Boston (Massachusetts), Glamorgan (Wales), Slovenia (Yugoslavia) and Tokyo (Japan) were 57.3%, 49.5%, 41.9% and 74.9%, respectively. In this report, data are presented on the types of treatment used in the four areas and on the relationship of differences in treatment practices to the differences in survival rates. Generally, surgically treated patients who also had radiotherapy had lower survival rates than patients in the same area who had similar operations without radiotherapy. In each area, the survival rate was higher for patients who had radical mastectomy than for those who had simple mastectomy. The Japanese patients had the highest survival rate within nearly every treatment and extent-treatment category. Thus, the high survival rate of these patients was not explained by the variables considered. Survival differences between Boston, Glamorgan and Slovenia were largely explained by differences in extent of disease and type of treatment. As the nature of the treatment--survival trends was consistent with the interpretation that treatment tended to be selected according to apparent prognosis, the degree to which treatment customs were determinants of the differences in survival rate among the three areas is uncertain.", "contents": "Some international differences in treatment and survival in breast cancer. In a recent study, 5-year survival rates for breast cancer patients in Boston (Massachusetts), Glamorgan (Wales), Slovenia (Yugoslavia) and Tokyo (Japan) were 57.3%, 49.5%, 41.9% and 74.9%, respectively. In this report, data are presented on the types of treatment used in the four areas and on the relationship of differences in treatment practices to the differences in survival rates. Generally, surgically treated patients who also had radiotherapy had lower survival rates than patients in the same area who had similar operations without radiotherapy. In each area, the survival rate was higher for patients who had radical mastectomy than for those who had simple mastectomy. The Japanese patients had the highest survival rate within nearly every treatment and extent-treatment category. Thus, the high survival rate of these patients was not explained by the variables considered. Survival differences between Boston, Glamorgan and Slovenia were largely explained by differences in extent of disease and type of treatment. As the nature of the treatment--survival trends was consistent with the interpretation that treatment tended to be selected according to apparent prognosis, the degree to which treatment customs were determinants of the differences in survival rate among the three areas is uncertain.", "PMID": 955744}
{"id": "pubmed23n0032_1767", "title": "The role of 9-hydroxybenzo(a)pyrene in the microsome mediated binding of benzo(a)pyrene to DNA.", "content": "A study of the liver microsome-mediated binding to added DNA of the phenol metabolites of benzo(a)pyrene (BP-OH) and of 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene (BP-7,8-diol) suggested that as in the case of BP itself the reaction was catalysed by the enzyme aryl hydrocarbon hydroxylase. The addition of glutathione to the microsomal incubation inhibited the binding of BP and BP-OH more than that of BP-7,8-diol. Analysis by LH20 chromatography of the deoxyribonucleoside products from BP-DNA showed greater inhibition by glutathione of formation of the major product believed to result from further metabolism of BP-OH, than of the product arising by metabolism of BP-7,8-diol. The chromatographic behaviour and fluorescence spectrum of this major product were consistent with its derivation from 9-hydroxybenzo(a)pyrene (BP-9-OH) and furthermore suggested that BP-9-OH-4,5-oxide was the derivative whose reaction with DNA yielded this microsome-mediated BP-DNA product.", "contents": "The role of 9-hydroxybenzo(a)pyrene in the microsome mediated binding of benzo(a)pyrene to DNA. A study of the liver microsome-mediated binding to added DNA of the phenol metabolites of benzo(a)pyrene (BP-OH) and of 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene (BP-7,8-diol) suggested that as in the case of BP itself the reaction was catalysed by the enzyme aryl hydrocarbon hydroxylase. The addition of glutathione to the microsomal incubation inhibited the binding of BP and BP-OH more than that of BP-7,8-diol. Analysis by LH20 chromatography of the deoxyribonucleoside products from BP-DNA showed greater inhibition by glutathione of formation of the major product believed to result from further metabolism of BP-OH, than of the product arising by metabolism of BP-7,8-diol. The chromatographic behaviour and fluorescence spectrum of this major product were consistent with its derivation from 9-hydroxybenzo(a)pyrene (BP-9-OH) and furthermore suggested that BP-9-OH-4,5-oxide was the derivative whose reaction with DNA yielded this microsome-mediated BP-DNA product.", "PMID": 955745}
{"id": "pubmed23n0032_1768", "title": "Demonstration of tumor-associated immunity with a leukocyte adherence inhibition (LAI) assay.", "content": "A modification of the leukocyte adherence inhibition (LAI) assay of Halliday was used to search for immune reactions against tumor-associated antigens of mouse tumors as well as against embryonic antigens in such neoplasms. Soluble antigen extracts were prepared from transplanted (BALB/c) methylcholanthrene-induced sarcomas and carcinomas, from normal BALB/c embryos taken at 14-18 days' gestation and from kidneys and livers of adult BALB/c mice. Peritoneal cells (PC) from mice immunized against syngeneic tumors gave leukocyte adherence inhibition more commonly when exposed to antigens prepared from the same tumor than did PC from normal (untreated) mice or from mice immunized against a different tumor. However, different tumor antigen preparations varied vastly in their ability to give specific adherence inhibition; some preparations consistently gave a high tumor-specific inhibition, while others did not. This may explain why the degree of reactivity observed was low and its tumor specificity not absolute when data obtained with all different antigen extracts were pooled. PC from multiparous mice gave adherence inhibition when exposed to antigenic extracts from syngenic mouse embryos or from tumors, as compared to PC from virgin mice exposed to the same extracts. Furthermore, PC from tumor-immunized mice reacted more commonly against antigen extracts from mouse embryos than did peritoneal cells from normal untreated mice. Adherence inhibition was not observed when PC from tumor-immunized or multiparous mice were exposed to antigen extracts from adult syngeneic livers or kidneys.", "contents": "Demonstration of tumor-associated immunity with a leukocyte adherence inhibition (LAI) assay. A modification of the leukocyte adherence inhibition (LAI) assay of Halliday was used to search for immune reactions against tumor-associated antigens of mouse tumors as well as against embryonic antigens in such neoplasms. Soluble antigen extracts were prepared from transplanted (BALB/c) methylcholanthrene-induced sarcomas and carcinomas, from normal BALB/c embryos taken at 14-18 days' gestation and from kidneys and livers of adult BALB/c mice. Peritoneal cells (PC) from mice immunized against syngeneic tumors gave leukocyte adherence inhibition more commonly when exposed to antigens prepared from the same tumor than did PC from normal (untreated) mice or from mice immunized against a different tumor. However, different tumor antigen preparations varied vastly in their ability to give specific adherence inhibition; some preparations consistently gave a high tumor-specific inhibition, while others did not. This may explain why the degree of reactivity observed was low and its tumor specificity not absolute when data obtained with all different antigen extracts were pooled. PC from multiparous mice gave adherence inhibition when exposed to antigenic extracts from syngenic mouse embryos or from tumors, as compared to PC from virgin mice exposed to the same extracts. Furthermore, PC from tumor-immunized mice reacted more commonly against antigen extracts from mouse embryos than did peritoneal cells from normal untreated mice. Adherence inhibition was not observed when PC from tumor-immunized or multiparous mice were exposed to antigen extracts from adult syngeneic livers or kidneys.", "PMID": 955746}
{"id": "pubmed23n0032_1769", "title": "The reaction of (+/-)-7alpha, 8beta-dihydroxy-9beta, 10beta-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene with DNA.", "content": "The reaction of (+/-)-7alpha,8beta-dihydroxy-9beta,10beta-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BP diol epoxide) with DNA, followed by enzymatic hydrolysis and LH 20 Sephadex column chromatography, yielded products containing a purine deoxyribonucleoside linked to a 7,8,9,10-tetrahydrobenzo(a)pyrene derivative. DNA containing tritiated adenine or pyrimidines gave little or no tritiated products, showing that reaction occurred chiefly with the guanine residues. Results with 8-(3H)-guanine-labelled DNA, and studies on the protonation and ionization of the products in acid or alkaline media, led to the formulation of the main product as an N2-substituted guanine derivative. The reaction of BP diol epoxide with deoxyadenosine and deoxyguanosine gave further nucleoside derivatives which were probably minor products in the reaction with DNA. Treatment with strong acid unexpectedly converted each of the isolated products to free guanine or adenine.", "contents": "The reaction of (+/-)-7alpha, 8beta-dihydroxy-9beta, 10beta-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene with DNA. The reaction of (+/-)-7alpha,8beta-dihydroxy-9beta,10beta-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BP diol epoxide) with DNA, followed by enzymatic hydrolysis and LH 20 Sephadex column chromatography, yielded products containing a purine deoxyribonucleoside linked to a 7,8,9,10-tetrahydrobenzo(a)pyrene derivative. DNA containing tritiated adenine or pyrimidines gave little or no tritiated products, showing that reaction occurred chiefly with the guanine residues. Results with 8-(3H)-guanine-labelled DNA, and studies on the protonation and ionization of the products in acid or alkaline media, led to the formulation of the main product as an N2-substituted guanine derivative. The reaction of BP diol epoxide with deoxyadenosine and deoxyguanosine gave further nucleoside derivatives which were probably minor products in the reaction with DNA. Treatment with strong acid unexpectedly converted each of the isolated products to free guanine or adenine.", "PMID": 955747}
{"id": "pubmed23n0032_1770", "title": "Intra-lesional injection of immunostimulants in bilateral rat tumors.", "content": "Rats grafted with either two or six fragments of isogenic methylcholanthrene-induced transplantable fibrosarcomas were treated IT1 with living BCG or killed C. parvum or a mixture of both immunostimulants. Various tumor combination and therapeutic agent dosages were compared. When two fragments of the same tumor McFiFi2 (S) were grafted simultaneously, IT treatment of one of these with 2 mg of BCG induced cure of both in 50% of the animals, but IT injection of 2 X 10(9) C. parvum was completely without effect in this situation. The prognosis was however, improved when the dose of immunostimulant was increased. The best results were obtained when each individual tumor in rats with two or six simultaneous identical grafts were treated with a mixture of BCG and C. parvum. The combination of IT immunostimulant treatment and surgical excision of the treated lesion demonstrated a persistence of the curative effect on the remote untreated tumor. Double grafting with isologous non-identical tumors revealed the influence of tumor burden and of the specificity of anti-tumor action of the treatment. The distant specific regression obtained in this system implies a specific immunological mechanism mediated by effector cells and/or antibodies which can circulate, identify the target cell and destroy it. This is in accordance with morphological observations. The intimate contact between BCG and the growing structured tumor is necessary for the therapeutic phenomenon.", "contents": "Intra-lesional injection of immunostimulants in bilateral rat tumors. Rats grafted with either two or six fragments of isogenic methylcholanthrene-induced transplantable fibrosarcomas were treated IT1 with living BCG or killed C. parvum or a mixture of both immunostimulants. Various tumor combination and therapeutic agent dosages were compared. When two fragments of the same tumor McFiFi2 (S) were grafted simultaneously, IT treatment of one of these with 2 mg of BCG induced cure of both in 50% of the animals, but IT injection of 2 X 10(9) C. parvum was completely without effect in this situation. The prognosis was however, improved when the dose of immunostimulant was increased. The best results were obtained when each individual tumor in rats with two or six simultaneous identical grafts were treated with a mixture of BCG and C. parvum. The combination of IT immunostimulant treatment and surgical excision of the treated lesion demonstrated a persistence of the curative effect on the remote untreated tumor. Double grafting with isologous non-identical tumors revealed the influence of tumor burden and of the specificity of anti-tumor action of the treatment. The distant specific regression obtained in this system implies a specific immunological mechanism mediated by effector cells and/or antibodies which can circulate, identify the target cell and destroy it. This is in accordance with morphological observations. The intimate contact between BCG and the growing structured tumor is necessary for the therapeutic phenomenon.", "PMID": 955748}
{"id": "pubmed23n0032_1771", "title": "The sickness impact profile: conceptual formulation and methodology for the development of a health status measure.", "content": "The development of a health status measure, the Sickness Impact Profile (SIP), is described in terms of both its conceptualization and methodology. The need for a health status measure that is sensitive and appropriate, based on sickness-related behavior, and culturally unbiased, is discussed. A model of sickness behavior is presented as a guide for methodological development. The description of the initial developmental stage of the SIP includeds detailed discussion and documentation of the collection, sorting and grouping of items that comprise the SIP, scaling of the items, scoring of the instrument, and testing and revision of the prototype instrument. Results of preliminary tests of reliability, validity, and administrative feasibility are presented. Subsequent steps in revision and finalization, now under way, are outlined.", "contents": "The sickness impact profile: conceptual formulation and methodology for the development of a health status measure. The development of a health status measure, the Sickness Impact Profile (SIP), is described in terms of both its conceptualization and methodology. The need for a health status measure that is sensitive and appropriate, based on sickness-related behavior, and culturally unbiased, is discussed. A model of sickness behavior is presented as a guide for methodological development. The description of the initial developmental stage of the SIP includeds detailed discussion and documentation of the collection, sorting and grouping of items that comprise the SIP, scaling of the items, scoring of the instrument, and testing and revision of the prototype instrument. Results of preliminary tests of reliability, validity, and administrative feasibility are presented. Subsequent steps in revision and finalization, now under way, are outlined.", "PMID": 955750}
{"id": "pubmed23n0032_1772", "title": "Unmet needs as sociomedical indicators.", "content": "This paper discusses the Meharry Medical College Study of Unmet Needs designed to measure the effectiveness of alternative health care delivery systems: (a) comprehensive care with broad outreach, (b) comprehensive care with limited outreach, and (c) traditional care. Unmet needs are defined as the differences between services judged necessary to deal appropriately with health problems and services actually received. The central hypothesis is that comprehensive health programs will be more effective than traditional care in reducing unmet needs. Unmet needs are viewed as measures of program outcome and are one of several types of sociomedical indicators which use factors other than biomedical or biological states as measures of outcome. The distinction is made between unmet needs are discussed and the relatively limited focus of these is contrasted with the more comprehensive Meharry approach. Household interviews and clinical examinations provide the data base for deriving professional judgements of unmet needs in the medical, dental, nursing, and social services areas. The Meharry work suggests several areas in which further work on unmet needs would be useful.", "contents": "Unmet needs as sociomedical indicators. This paper discusses the Meharry Medical College Study of Unmet Needs designed to measure the effectiveness of alternative health care delivery systems: (a) comprehensive care with broad outreach, (b) comprehensive care with limited outreach, and (c) traditional care. Unmet needs are defined as the differences between services judged necessary to deal appropriately with health problems and services actually received. The central hypothesis is that comprehensive health programs will be more effective than traditional care in reducing unmet needs. Unmet needs are viewed as measures of program outcome and are one of several types of sociomedical indicators which use factors other than biomedical or biological states as measures of outcome. The distinction is made between unmet needs are discussed and the relatively limited focus of these is contrasted with the more comprehensive Meharry approach. Household interviews and clinical examinations provide the data base for deriving professional judgements of unmet needs in the medical, dental, nursing, and social services areas. The Meharry work suggests several areas in which further work on unmet needs would be useful.", "PMID": 955751}
{"id": "pubmed23n0032_1773", "title": "Evaluation of health care quality by consumers.", "content": "Ideological and conceptual issues arising out of the involvement of consumers in health care quality determination are identified. Historically and currently, criteria and procedures for quality review and assessment have reflected the values and interests of the professional providers of health care. This perspective restricts the role of the consumer to that of either a nonparticipant or a data source, at best. If the social values and merits for health care provision of consumer involvement in quality determination are to be realized, then this perspective will need to be modified, and our knowledge base of consumer criteria of health care quality will need to be enlarged. Although the perspectives of probiders and consumers are thought to the antagonistic, they may not, in fact, be so. Some suggested areas of needed research, necessary to policy development and implementation, are offered.", "contents": "Evaluation of health care quality by consumers. Ideological and conceptual issues arising out of the involvement of consumers in health care quality determination are identified. Historically and currently, criteria and procedures for quality review and assessment have reflected the values and interests of the professional providers of health care. This perspective restricts the role of the consumer to that of either a nonparticipant or a data source, at best. If the social values and merits for health care provision of consumer involvement in quality determination are to be realized, then this perspective will need to be modified, and our knowledge base of consumer criteria of health care quality will need to be enlarged. Although the perspectives of probiders and consumers are thought to the antagonistic, they may not, in fact, be so. Some suggested areas of needed research, necessary to policy development and implementation, are offered.", "PMID": 955752}
{"id": "pubmed23n0032_1774", "title": "Constructing social metrics for health status indexes.", "content": "Health status indexes used to make collective decisions satisfying the principles of equality and social minimum must incorporate a social metric for health. Any index or indicator applied to populations for determining health status or to health programs for evaluating outcome must confront the question of who prefers which states of health under which circumstances? Utility models, psychometric scaling, and empirical social decision valuation have been used to measure preferences for states of health. Efforts should be directed toward constructing social metrics for health that are prospective, context-independent, relevant, community-wide, ratio scalled, sensitive, empirically validated, and applicable to program evaluation. These efforts represent the application of normative social theory to research, an important advance in uncovering the mysteries of social action and its consequences.", "contents": "Constructing social metrics for health status indexes. Health status indexes used to make collective decisions satisfying the principles of equality and social minimum must incorporate a social metric for health. Any index or indicator applied to populations for determining health status or to health programs for evaluating outcome must confront the question of who prefers which states of health under which circumstances? Utility models, psychometric scaling, and empirical social decision valuation have been used to measure preferences for states of health. Efforts should be directed toward constructing social metrics for health that are prospective, context-independent, relevant, community-wide, ratio scalled, sensitive, empirically validated, and applicable to program evaluation. These efforts represent the application of normative social theory to research, an important advance in uncovering the mysteries of social action and its consequences.", "PMID": 955753}
{"id": "pubmed23n0032_1775", "title": "Reproductive efficiency as a social indicator.", "content": "An index of reproductive effiency (RE) is proposed as a social indicator that will meet the need to consider various forms of pregnancy wastage, to compare their relative costs, and to guide reproductive health policy accordingly. This article discusses conceptual and measurement aspects of RE. Conversion of wanted to unwanted pregnancies and the reverse, interpretation of abortion in relation to other pregnancy outcomes, defining the end point for the reproductive process and criteria for the events to be included as significant outcomes are conceptual issues. Measurement problems include: whether aggregation is justified, prospective and retrospective tracking of outcomes, record limitations, duplication of adversities in a single pregnancy, and selection of optimal rate for comparison. A measurement of RE for the entire United States based on the National Natality Survey of 1964-1966 is presented, showing 74.5 percent of pregnancies resulting in healthy liveborn infants. For those years, data on abortions could not be included. Within the group of reported pregnancy losses, the importance of congenital abnormalities and low-birth-weight babies is enhanced by application of economic weights based on associated medical care costs. Changing opportunities for birth timing, prenatal and infant care, and control of family size are social means of reducing adverse outcomes associated with teenage pregnancy and high-parity births, often found together with poverty. Successive increments in RE may be progressively more expensive to achieve, and cost effectiveness comparison will be necessary.", "contents": "Reproductive efficiency as a social indicator. An index of reproductive effiency (RE) is proposed as a social indicator that will meet the need to consider various forms of pregnancy wastage, to compare their relative costs, and to guide reproductive health policy accordingly. This article discusses conceptual and measurement aspects of RE. Conversion of wanted to unwanted pregnancies and the reverse, interpretation of abortion in relation to other pregnancy outcomes, defining the end point for the reproductive process and criteria for the events to be included as significant outcomes are conceptual issues. Measurement problems include: whether aggregation is justified, prospective and retrospective tracking of outcomes, record limitations, duplication of adversities in a single pregnancy, and selection of optimal rate for comparison. A measurement of RE for the entire United States based on the National Natality Survey of 1964-1966 is presented, showing 74.5 percent of pregnancies resulting in healthy liveborn infants. For those years, data on abortions could not be included. Within the group of reported pregnancy losses, the importance of congenital abnormalities and low-birth-weight babies is enhanced by application of economic weights based on associated medical care costs. Changing opportunities for birth timing, prenatal and infant care, and control of family size are social means of reducing adverse outcomes associated with teenage pregnancy and high-parity births, often found together with poverty. Successive increments in RE may be progressively more expensive to achieve, and cost effectiveness comparison will be necessary.", "PMID": 955754}
{"id": "pubmed23n0032_1776", "title": "Indicators of health status in adolescence.", "content": "The study reported here is based on data obtained in 1968-1970 from a representative community sample of urban black youths in the United States aged 12-17 years, inclusive. Analysis is directed at conceptual and methodological issues in measureing health status. It suggests the need for greater attention to subjective self-evaluated self-reported components of health status, specified here as \"ontological\" health. This is related to health and illness behavior generally, to utilization of health services more particularly. The case is made for a multiple-indicator approach to measuring health status as being more consistent with the multidimensional phenomenon to which it refers. The method used in this study for deriving a composite health status index from four component self-reported indicators is described. The distribution of the sample on this composite was used to identify self-reported health conditions that warrant attention from providers of adolescent health services. Since subjective evaluations influence experienced severity of health problems, the health status composite index was applied in this study as a means of discriminating differntial seriousness in self-reported health problems. Finally, some differences between indications of \"ontological\" health and \"medical\" health also are analyzed for commonalities and differences between them.", "contents": "Indicators of health status in adolescence. The study reported here is based on data obtained in 1968-1970 from a representative community sample of urban black youths in the United States aged 12-17 years, inclusive. Analysis is directed at conceptual and methodological issues in measureing health status. It suggests the need for greater attention to subjective self-evaluated self-reported components of health status, specified here as \"ontological\" health. This is related to health and illness behavior generally, to utilization of health services more particularly. The case is made for a multiple-indicator approach to measuring health status as being more consistent with the multidimensional phenomenon to which it refers. The method used in this study for deriving a composite health status index from four component self-reported indicators is described. The distribution of the sample on this composite was used to identify self-reported health conditions that warrant attention from providers of adolescent health services. Since subjective evaluations influence experienced severity of health problems, the health status composite index was applied in this study as a means of discriminating differntial seriousness in self-reported health problems. Finally, some differences between indications of \"ontological\" health and \"medical\" health also are analyzed for commonalities and differences between them.", "PMID": 955755}
{"id": "pubmed23n0032_1777", "title": "Comments on health indicators: methodological perspectives.", "content": "This paper discusses several basic conceptual and methodological problems in the development and use of health indicators. We observe that two tendencies of the health indicators movement may deter progress toward producing informations of health status indexes and the tendency to conceptualize \"health\" in terms of expansive definitions should measure variables specified by a social system model and should be scaled according to units that are relevant to decision-making criteria.", "contents": "Comments on health indicators: methodological perspectives. This paper discusses several basic conceptual and methodological problems in the development and use of health indicators. We observe that two tendencies of the health indicators movement may deter progress toward producing informations of health status indexes and the tendency to conceptualize \"health\" in terms of expansive definitions should measure variables specified by a social system model and should be scaled according to units that are relevant to decision-making criteria.", "PMID": 955756}
{"id": "pubmed23n0032_1778", "title": "Social and behavioural aspects of venereal disease among resident male university students.", "content": "A study of 1500 male students at Banaras Hindu University, Varanasi, India was conducted to establish the prevalence and related social and behavioural aspects of venereal diseases. The prevalence was found to be 3.93 per cent. The majority of the students (86.4 per cent) belonged to the Hindu religion which is based on the caste system. Students from the Vaishya caste were more affected with venereal diseases. The social acceptability of having more than one wife had a definite impact on the incidence of venereal diseases. Students who practised masturbation and homosexuality were also more affected with venereal diseases. Prostitutes were the main source of infection. It was found that 28.8 per cent of these students had been infected on a previous occasion.", "contents": "Social and behavioural aspects of venereal disease among resident male university students. A study of 1500 male students at Banaras Hindu University, Varanasi, India was conducted to establish the prevalence and related social and behavioural aspects of venereal diseases. The prevalence was found to be 3.93 per cent. The majority of the students (86.4 per cent) belonged to the Hindu religion which is based on the caste system. Students from the Vaishya caste were more affected with venereal diseases. The social acceptability of having more than one wife had a definite impact on the incidence of venereal diseases. Students who practised masturbation and homosexuality were also more affected with venereal diseases. Prostitutes were the main source of infection. It was found that 28.8 per cent of these students had been infected on a previous occasion.", "PMID": 955759}
{"id": "pubmed23n0032_1779", "title": "Response variation and location of questions within a questionnaire.", "content": "In a questionnaire administered to a general population sample of 1673 adults, a 14-item scale of social desirability was divided into two groups of questions, almost evenly divided between desirable and undesirable behaviour, the percentage answering 'true' was higher when the question was asked early than when it was asked late. The difference was statistically significant for five of the 11, four of them relating to socially undesirable behaviour. The results indicate that responses can be dependent on the location of questions in a questionnaire, and that the effect of question location may not be the same for groups with different personal characteristics. For this reason, it seems unwise to alter the administration or design of a questionnaire in any way if the results are to be compared directly to those of other studies, unless the altered questionnaire is standardized against the original version.", "contents": "Response variation and location of questions within a questionnaire. In a questionnaire administered to a general population sample of 1673 adults, a 14-item scale of social desirability was divided into two groups of questions, almost evenly divided between desirable and undesirable behaviour, the percentage answering 'true' was higher when the question was asked early than when it was asked late. The difference was statistically significant for five of the 11, four of them relating to socially undesirable behaviour. The results indicate that responses can be dependent on the location of questions in a questionnaire, and that the effect of question location may not be the same for groups with different personal characteristics. For this reason, it seems unwise to alter the administration or design of a questionnaire in any way if the results are to be compared directly to those of other studies, unless the altered questionnaire is standardized against the original version.", "PMID": 955760}
{"id": "pubmed23n0032_1780", "title": "Birth order, maternal age and birth interval in epidemiology.", "content": "Foetal wastage and congenital malformations are not so closely associated with birth  order, maternal age and birth interval as is suggested by data pooled across sibships of different sizes. The point could only have been established by data on large numbers of individual sibships. Maternal age, parity and birth interval are convenient but confusing variables to work with. However if they are to be used, it is essential to recognize that if the condition being studied may be associated with fertility of the parents, one needs to ascertain numbers of completed sibships containing affected cases. Incidence rates by age, parity and birth interval imply nothing by themselves.", "contents": "Birth order, maternal age and birth interval in epidemiology. Foetal wastage and congenital malformations are not so closely associated with birth  order, maternal age and birth interval as is suggested by data pooled across sibships of different sizes. The point could only have been established by data on large numbers of individual sibships. Maternal age, parity and birth interval are convenient but confusing variables to work with. However if they are to be used, it is essential to recognize that if the condition being studied may be associated with fertility of the parents, one needs to ascertain numbers of completed sibships containing affected cases. Incidence rates by age, parity and birth interval imply nothing by themselves.", "PMID": 955761}
{"id": "pubmed23n0032_1781", "title": "Aspects of the epidemiology of suicide in England and Wales.", "content": "This paper is concerned with the variations in suicide rates in the 161-171 local authorities in England and Wales during the 11-year period 1961-71. Analysis of the overall secular trends shows that the rates for both males and females reached a peak in 1963 and subsequently declined at a steady rate. Virtually the whole of this reduction is associated with the fall in the number of poisonings by domestic gas. Suicide rates were about 50 per cent higher in males than in females and tended to increase with increasing age and, in general, were higher in the county boroughs than in the (more rural) county authorities. There is a marked variation in terms of geographical area, with the highest rates being reported in the London and South-East Region and the lowest rates in the South-East Wales and Northern Regions. There are also systematic differences between the various conurbations, with the highest rates in London and South-East Lancashire and the lowest in Merseyside and the West Midlands. The variations between individual local authorities are then considered and it is shown that as much as 99 per cent of the total variation can be explained in terms of a small number of variables which represent the general characteristics of the the local authority and the population. The most important of these variables is the proportion of single-person housegolds, which accounts for about three-quarters of the variation in the male suicide rates and more than half of the variation in female suicide rates. The significance of these results is discussed, with particular reference to the effect of the detoxification of the domestic gas supply during the period of study.", "contents": "Aspects of the epidemiology of suicide in England and Wales. This paper is concerned with the variations in suicide rates in the 161-171 local authorities in England and Wales during the 11-year period 1961-71. Analysis of the overall secular trends shows that the rates for both males and females reached a peak in 1963 and subsequently declined at a steady rate. Virtually the whole of this reduction is associated with the fall in the number of poisonings by domestic gas. Suicide rates were about 50 per cent higher in males than in females and tended to increase with increasing age and, in general, were higher in the county boroughs than in the (more rural) county authorities. There is a marked variation in terms of geographical area, with the highest rates being reported in the London and South-East Region and the lowest rates in the South-East Wales and Northern Regions. There are also systematic differences between the various conurbations, with the highest rates in London and South-East Lancashire and the lowest in Merseyside and the West Midlands. The variations between individual local authorities are then considered and it is shown that as much as 99 per cent of the total variation can be explained in terms of a small number of variables which represent the general characteristics of the the local authority and the population. The most important of these variables is the proportion of single-person housegolds, which accounts for about three-quarters of the variation in the male suicide rates and more than half of the variation in female suicide rates. The significance of these results is discussed, with particular reference to the effect of the detoxification of the domestic gas supply during the period of study.", "PMID": 955762}
{"id": "pubmed23n0032_1782", "title": "Suicide and self-poisoning in Great Britain and Ireland.", "content": "Reported national rates of suicide remain fairly stable from year to year and considerable differences persist between countries. Reported rates of suicide also differ from actual rates according to the social influences and the legal procedure both in attributing suicide and also in how the death is recorded and coded. This paper compares the rates of suicide and non-fatal self-poisoning in Scotland, England and Wales, and Ireland, and also examines suicide rates in people born in Ireland but now resident in England and Wales. The rise in overdose admissions to hospital which is continuing in relative degrees in all communities of Great Britain and Ireland, except Northern Ireland, does not seem to be correlated with trends in fatal suicide behaviour. Further studies on all aspects of the subject are needed to clarify the social determinants involved and to identify ways in which further increases can be contained.", "contents": "Suicide and self-poisoning in Great Britain and Ireland. Reported national rates of suicide remain fairly stable from year to year and considerable differences persist between countries. Reported rates of suicide also differ from actual rates according to the social influences and the legal procedure both in attributing suicide and also in how the death is recorded and coded. This paper compares the rates of suicide and non-fatal self-poisoning in Scotland, England and Wales, and Ireland, and also examines suicide rates in people born in Ireland but now resident in England and Wales. The rise in overdose admissions to hospital which is continuing in relative degrees in all communities of Great Britain and Ireland, except Northern Ireland, does not seem to be correlated with trends in fatal suicide behaviour. Further studies on all aspects of the subject are needed to clarify the social determinants involved and to identify ways in which further increases can be contained.", "PMID": 955763}
{"id": "pubmed23n0032_1783", "title": "Occurrence of gout in Copenhagen males aged 40-59.", "content": "As part of a cardiovascular survey study on the relationships between physical fitness and coronary heart disease, 5249 Copenhagen males aged between 40 and 59 were interviewed to identify a history of gout. Subjects who had experienced attacks of painful swelling, with abrupt onset and remission in one to two weeks, diagnosed and treated as gout by their own physician, were regarded as having experienced gout. In an initial cross-sectional examination, 86 men fulfilled the criteria. At a one year follow-up examination it was discovered that 56 men had had gout during the year of observation. Among these 18 were new cases. At the end of the study a total of 104 men had experienced gout and these were compared, with respect to the continuously distributed variables, those 104 gout subjects were compared to 208 computer selected age-matched controls drawn at random from the entire sample. The gout cases were found to have higher relative weights, higher diastolic blood pressure and lower levels of physical fitness estimated by use of a bicycle ergometer test. Angina pectoris occurred more frequently among the gout cases than among controls. No significant differences in the occurrence of myocardial infarction, intermittent claudication or renal stones were found. The habits of smoking and coffee consumption were equal in gout cases and controls. Alcohol consumption and consumption of drugs were higher in gout cases than controls. Gout was the most frequent in the lowest social classes.", "contents": "Occurrence of gout in Copenhagen males aged 40-59. As part of a cardiovascular survey study on the relationships between physical fitness and coronary heart disease, 5249 Copenhagen males aged between 40 and 59 were interviewed to identify a history of gout. Subjects who had experienced attacks of painful swelling, with abrupt onset and remission in one to two weeks, diagnosed and treated as gout by their own physician, were regarded as having experienced gout. In an initial cross-sectional examination, 86 men fulfilled the criteria. At a one year follow-up examination it was discovered that 56 men had had gout during the year of observation. Among these 18 were new cases. At the end of the study a total of 104 men had experienced gout and these were compared, with respect to the continuously distributed variables, those 104 gout subjects were compared to 208 computer selected age-matched controls drawn at random from the entire sample. The gout cases were found to have higher relative weights, higher diastolic blood pressure and lower levels of physical fitness estimated by use of a bicycle ergometer test. Angina pectoris occurred more frequently among the gout cases than among controls. No significant differences in the occurrence of myocardial infarction, intermittent claudication or renal stones were found. The habits of smoking and coffee consumption were equal in gout cases and controls. Alcohol consumption and consumption of drugs were higher in gout cases than controls. Gout was the most frequent in the lowest social classes.", "PMID": 955764}
{"id": "pubmed23n0032_1784", "title": "Psycho-social effects of home care: results of a randomized controlled trial.", "content": "A randomized controlled trial was conducted to evaluate the efficacy of the early transfer of hospital in-patients to home care. This paper reports rates of return to normal role functions in work, leisure and household roles for patients who received any of five surgical procedures. With regard to return to work or leisure tasks, no statistically significant differences were observed between groups for any of the procedures. The rates of return to household activities were greater for home care patients than they were for controls in the cases of cholecystectomy and procedures for anal and rectal fistula.", "contents": "Psycho-social effects of home care: results of a randomized controlled trial. A randomized controlled trial was conducted to evaluate the efficacy of the early transfer of hospital in-patients to home care. This paper reports rates of return to normal role functions in work, leisure and household roles for patients who received any of five surgical procedures. With regard to return to work or leisure tasks, no statistically significant differences were observed between groups for any of the procedures. The rates of return to household activities were greater for home care patients than they were for controls in the cases of cholecystectomy and procedures for anal and rectal fistula.", "PMID": 955765}
{"id": "pubmed23n0032_1785", "title": "A classification of sociomedical health indicators: perspectives for health administrators and health planners.", "content": "The conceptualization and operationalization of measures of health status are considered. Health indicators are conceived as a subset of social indicators, and therefore, as any social indicator, they are viewed as derivative from social issues. The interrelationships of different frames of reference for defining and measuring health that have accompained three distinct health problem patterns in the United States are viewed from a developmental perspective. Mortality and morbidity rates, the traditional health indicators, by themselves no longer serve to assess health status in developed nations. Their deficiencies as indicators serve as background for a classification schema for sociomedical health status indicators that relates health definition frames of reference, measures of health status, and health problems. The role of a group of health indicators-sociomedical heath indicators-in the current formulation of health status measures is assessed.", "contents": "A classification of sociomedical health indicators: perspectives for health administrators and health planners. The conceptualization and operationalization of measures of health status are considered. Health indicators are conceived as a subset of social indicators, and therefore, as any social indicator, they are viewed as derivative from social issues. The interrelationships of different frames of reference for defining and measuring health that have accompained three distinct health problem patterns in the United States are viewed from a developmental perspective. Mortality and morbidity rates, the traditional health indicators, by themselves no longer serve to assess health status in developed nations. Their deficiencies as indicators serve as background for a classification schema for sociomedical health status indicators that relates health definition frames of reference, measures of health status, and health problems. The role of a group of health indicators-sociomedical heath indicators-in the current formulation of health status measures is assessed.", "PMID": 955757}
{"id": "pubmed23n0032_1786", "title": "Familial aggregation of chronic respiratory disease: use of National Health Interview Survey data for specific hypothesis testing.", "content": "The 1970 National Health Interview Survey included questions on respiratory disease and smoking habits. The new data were released in July 1974. Data consisted of information on approximately 116,000 persons from 37,000 households selected randomly from 357 primary sampling units. To test a hypothesis about familial clustering of chronic respiratory disease in households, we selected as index households those having an adult (aged 35-54 years) reporting a diagnosis of asthma, bronchitis, or emphysema and also having first order relatiaves less than age 35. Index households were matched with households from the same neighbourhood having an adult aged 35-54, of the same sex as the diseased person in the index household, without disease and with all other adults 35-54 without disease, and which had first order relatives less than 35 living in the same household. Analysis was carried out using Cochran's d-test to compare frequency of respiratory disease in persons less than 35 in each group. There was a strong association (P less than .001) between persons over 35 with chronic respiratory diseases and the disease rate in their first order relatives. The association could not be explained by differences in demographic variables and smoking habits.", "contents": "Familial aggregation of chronic respiratory disease: use of National Health Interview Survey data for specific hypothesis testing. The 1970 National Health Interview Survey included questions on respiratory disease and smoking habits. The new data were released in July 1974. Data consisted of information on approximately 116,000 persons from 37,000 households selected randomly from 357 primary sampling units. To test a hypothesis about familial clustering of chronic respiratory disease in households, we selected as index households those having an adult (aged 35-54 years) reporting a diagnosis of asthma, bronchitis, or emphysema and also having first order relatiaves less than age 35. Index households were matched with households from the same neighbourhood having an adult aged 35-54, of the same sex as the diseased person in the index household, without disease and with all other adults 35-54 without disease, and which had first order relatives less than 35 living in the same household. Analysis was carried out using Cochran's d-test to compare frequency of respiratory disease in persons less than 35 in each group. There was a strong association (P less than .001) between persons over 35 with chronic respiratory diseases and the disease rate in their first order relatives. The association could not be explained by differences in demographic variables and smoking habits.", "PMID": 955766}
{"id": "pubmed23n0032_1787", "title": "Changes in respiratory symptoms related to smoking in a teenage population: the results of two linked surveys separated by one year.", "content": "Data on respiratory symptoms and smoking for 2749 white American high school students from two linked surveys, separated by a one year lapse, are presented. There was a significantly higher likelihood of smoking at second survey among those who had initial symptoms. (There had been no educational efforts linking smoking and respiratory symptoms in the interim.) This difference was almost entirely contributed by those who had been ex-smokers and light (less than or equal to four cigarettes/day) smokers at first survey. Initial ex-smokers and light smokers also had, overall, least fixed smoking habits. Whatever the initial smoking or symptom status, symptoms at second survey were far more likely with current smoking. Controlling for initial symptom and smoking status, the gradient of symptoms between current smokers and non-smokers was over two and a half fold, except among those who had had symptoms and had not been smoking, and whose initial symptoms presumably arose from causes other than current cigarette smoking. Even among that group, symptoms at second survey were half as likely again among those who reported having become smokers (p less than-001). There were also strong linear trends of increased likelihood of symptoms with increased numbers of cigarettes smoked. Symptom rates at second survey of those who had symptoms a year earlier, and who stopped smoking during the year, were not as low as for those who also stopped smoking, but who had not had initial symptoms, suggesting that symptoms associated with smoking were not completely reversible in the time interval of this study. The difference, however, was not significant.", "contents": "Changes in respiratory symptoms related to smoking in a teenage population: the results of two linked surveys separated by one year. Data on respiratory symptoms and smoking for 2749 white American high school students from two linked surveys, separated by a one year lapse, are presented. There was a significantly higher likelihood of smoking at second survey among those who had initial symptoms. (There had been no educational efforts linking smoking and respiratory symptoms in the interim.) This difference was almost entirely contributed by those who had been ex-smokers and light (less than or equal to four cigarettes/day) smokers at first survey. Initial ex-smokers and light smokers also had, overall, least fixed smoking habits. Whatever the initial smoking or symptom status, symptoms at second survey were far more likely with current smoking. Controlling for initial symptom and smoking status, the gradient of symptoms between current smokers and non-smokers was over two and a half fold, except among those who had had symptoms and had not been smoking, and whose initial symptoms presumably arose from causes other than current cigarette smoking. Even among that group, symptoms at second survey were half as likely again among those who reported having become smokers (p less than-001). There were also strong linear trends of increased likelihood of symptoms with increased numbers of cigarettes smoked. Symptom rates at second survey of those who had symptoms a year earlier, and who stopped smoking during the year, were not as low as for those who also stopped smoking, but who had not had initial symptoms, suggesting that symptoms associated with smoking were not completely reversible in the time interval of this study. The difference, however, was not significant.", "PMID": 955767}
{"id": "pubmed23n0032_1788", "title": "Survival experience of a cohort of carbon disulphide exposed workers from an eight-year prospective follow-up period.", "content": "A cohort of 343 men from a viscose rayon factory in Finland with at least five years of exposure in carbon disulphide (CS2) was followed from 1967 to 1975 with respect to coronary heart disease (CHD) mortality. The exposed cohort had over twofold excess death rate from CHD relative to a matched reference group. Statistical separation of causes other than CHD revealed a difference of two years in the life expectancies of the two populations for the age interval of 25 to 54 years. The coronary mortality had, however, decreased since protective measures were instituted at the plant after the fifth year of follow-up, leaving only one-fifth of the original group exposed and reducing the level of CS2 exposure for them to less than 10 ppm. This trend, although tentative due to the limited experience after the intervention, seems to suggest that the risk of coronary death for the formerly excessively exposed workers can be influenced and lessened to a normal level.", "contents": "Survival experience of a cohort of carbon disulphide exposed workers from an eight-year prospective follow-up period. A cohort of 343 men from a viscose rayon factory in Finland with at least five years of exposure in carbon disulphide (CS2) was followed from 1967 to 1975 with respect to coronary heart disease (CHD) mortality. The exposed cohort had over twofold excess death rate from CHD relative to a matched reference group. Statistical separation of causes other than CHD revealed a difference of two years in the life expectancies of the two populations for the age interval of 25 to 54 years. The coronary mortality had, however, decreased since protective measures were instituted at the plant after the fifth year of follow-up, leaving only one-fifth of the original group exposed and reducing the level of CS2 exposure for them to less than 10 ppm. This trend, although tentative due to the limited experience after the intervention, seems to suggest that the risk of coronary death for the formerly excessively exposed workers can be influenced and lessened to a normal level.", "PMID": 955768}
{"id": "pubmed23n0032_1789", "title": "Recent trends of large bowel cancer in Japan compared to United States and England and Wales.", "content": "The death rate from tumours of the colon and rectum has risen rapidly in Japan since World War II. The rate of increase is greater in tumours of colon than in tumours of rectum. It is similar in males and females. It is large among the elderly, and this may be due to improvements in certification. But the difference between the Japanese and the US population is substantial at all ages. It is not known whether the current increase in the mortality of Japanese from tumours of the large bowel is due to differences between birth cohorts established early in life, or whether the differences developed after the end of World War II. Japanese who die of these tumours are younger than Americans, even when the age distribution of the populations is allowed for. This effect is produced by the differences between cohorts, and the age distributions within cohorts are closely similar in the two populations. The sex ratio for tumours of rectum rises with age in Japan in the same way that it does in the West, but the sex ratio of tumours of colon is apparently independent of age.", "contents": "Recent trends of large bowel cancer in Japan compared to United States and England and Wales. The death rate from tumours of the colon and rectum has risen rapidly in Japan since World War II. The rate of increase is greater in tumours of colon than in tumours of rectum. It is similar in males and females. It is large among the elderly, and this may be due to improvements in certification. But the difference between the Japanese and the US population is substantial at all ages. It is not known whether the current increase in the mortality of Japanese from tumours of the large bowel is due to differences between birth cohorts established early in life, or whether the differences developed after the end of World War II. Japanese who die of these tumours are younger than Americans, even when the age distribution of the populations is allowed for. This effect is produced by the differences between cohorts, and the age distributions within cohorts are closely similar in the two populations. The sex ratio for tumours of rectum rises with age in Japan in the same way that it does in the West, but the sex ratio of tumours of colon is apparently independent of age.", "PMID": 955769}
{"id": "pubmed23n0032_1790", "title": "Mortality from ischaemic heart disease--inter-town variation and its association with climate in England and Wales.", "content": "In both seasonal and inter-town variation, the mortality from ischaemic heart disease in England and Wales in 1969-71 is very highly correlated with temperature. Also in the inter-town variation, IHD mortality is highly correlated with rainfall and with socio-economic index. It is postulated that the same mechanism of body cooling underlies seasonal, inter-town and socio-economic variations in IHD mortality. The first law of thermodynamics states the heat and energy are equivalent. Therefore to maintain body temperature against an increased thermal gradient (caused by a lower environmental temperature as in seasonal variation, or a temperature-rainfall interaction as in inter-town variation) more energy must be expended by the body or, alternatively, the body must reduce heat loss by increasing thermal insulation. It is to be expected that there will be many accompanying changes in physiological parameters and several of these are discussed.", "contents": "Mortality from ischaemic heart disease--inter-town variation and its association with climate in England and Wales. In both seasonal and inter-town variation, the mortality from ischaemic heart disease in England and Wales in 1969-71 is very highly correlated with temperature. Also in the inter-town variation, IHD mortality is highly correlated with rainfall and with socio-economic index. It is postulated that the same mechanism of body cooling underlies seasonal, inter-town and socio-economic variations in IHD mortality. The first law of thermodynamics states the heat and energy are equivalent. Therefore to maintain body temperature against an increased thermal gradient (caused by a lower environmental temperature as in seasonal variation, or a temperature-rainfall interaction as in inter-town variation) more energy must be expended by the body or, alternatively, the body must reduce heat loss by increasing thermal insulation. It is to be expected that there will be many accompanying changes in physiological parameters and several of these are discussed.", "PMID": 955770}
{"id": "pubmed23n0032_1791", "title": "Laser Raman spectroscopy of snake venom neurotoxins: conformation.", "content": "Laser Raman spectra of neurotoxins of Pelamis platurus (yellow-bellied sea snake) and Laticauda semifasciata (broad-banded blue sea snake) were investigated. The amide I band appeared at 1672 cm-1 for both toxins, which presents an indication of anti-parallel beta structure. Since this agrees well with the result from the CD-ORD studies of snake neurotoxin, it was concluded that snake neurotoxins mainly consist of beta structure. The amide III band appeared at 1245 cm-1 for P. platurus toxin and 1248 cm-1 for L. semifasciata toxin. The four disulfide bonds present in the toxin have a very similar geometry. After vigorous heat treatment, the backbone configuration of the toxin molecule basically remained the same although it was partially denatured. The major peak at 512 cm-1 was not altered by the heat treatment but a new shoulder appeared at 546 cm-1. This suggests that a new type of S-S stretching vibration (trans-gauche-trans) was produced as a result of heat treatment. However, the majority of the S-S vibrations remained in the gauche-gauche-gauche orientation. A substantial change in the interactions between a tyrosine aromatic ring and neighboring residues was apparently the alteration caused by the heat treatment.", "contents": "Laser Raman spectroscopy of snake venom neurotoxins: conformation. Laser Raman spectra of neurotoxins of Pelamis platurus (yellow-bellied sea snake) and Laticauda semifasciata (broad-banded blue sea snake) were investigated. The amide I band appeared at 1672 cm-1 for both toxins, which presents an indication of anti-parallel beta structure. Since this agrees well with the result from the CD-ORD studies of snake neurotoxin, it was concluded that snake neurotoxins mainly consist of beta structure. The amide III band appeared at 1245 cm-1 for P. platurus toxin and 1248 cm-1 for L. semifasciata toxin. The four disulfide bonds present in the toxin have a very similar geometry. After vigorous heat treatment, the backbone configuration of the toxin molecule basically remained the same although it was partially denatured. The major peak at 512 cm-1 was not altered by the heat treatment but a new shoulder appeared at 546 cm-1. This suggests that a new type of S-S stretching vibration (trans-gauche-trans) was produced as a result of heat treatment. However, the majority of the S-S vibrations remained in the gauche-gauche-gauche orientation. A substantial change in the interactions between a tyrosine aromatic ring and neighboring residues was apparently the alteration caused by the heat treatment.", "PMID": 955780}
{"id": "pubmed23n0032_1792", "title": "Studies on the primary structure of bison pancreatic ribonuclease.", "content": "Bison pancreatic ribonuclease was isolated by affinity chromatography. Thermolysin and tryptic digestion of denaturated protein, and subtilisin digestion of native protein yielded peptides, which were purified and submitted to amino acid analysis. These peptides, together with partial sequence data obtained by Stewart & Stevenson (16) overlap the entire amino acid sequence of bison ribonuclease. No differences with bovine ribonuclease were found, although there may be differences in state of amidation of some residues.", "contents": "Studies on the primary structure of bison pancreatic ribonuclease. Bison pancreatic ribonuclease was isolated by affinity chromatography. Thermolysin and tryptic digestion of denaturated protein, and subtilisin digestion of native protein yielded peptides, which were purified and submitted to amino acid analysis. These peptides, together with partial sequence data obtained by Stewart & Stevenson (16) overlap the entire amino acid sequence of bison ribonuclease. No differences with bovine ribonuclease were found, although there may be differences in state of amidation of some residues.", "PMID": 955781}
{"id": "pubmed23n0032_1793", "title": "Structure-activity studies on mammalian tissue lytic enzymes: chemical characterization and substrate specificity of rat kidney nuclear lysozyme.", "content": "Studies on the structure and substrate specificity of purified rat kidney nuclear (RKN) lysozyme are reported. The carboxyl and amino terminal residues of RKN-lysozyme were found to be leucine and alanine respectively. The amino acid composition indicated similarities and differences as compared with that of hen egg white (HEW) lysozyme. There were alterations in the nine amino acid residues, Lys, His, Arg, Asp, Glu, Pro, 1/2 Cys, Tyr and Trp. The other nine residues were present in identical proportions to those of HEW-lysozyme. The decrease in the arginine and aspartic acid residues was found to be compensated by the increase in the number of lysine, histidine and glutamic acid residues. The overall ratio of the acidic to basic amino acids has thus been conserved in the mammalian enzyme. In addition, RKN-lysozyme contained decreased numbers of Trp, Tyr and 1/2 Cys, and increased numbers of proline residues as found in HEW-lysozyme. RKN-lysozyme did not cross react with heterologous antibodies produced against HEW-lysozyme, and vice versa. RKN-lysozyme showed distinct specificity towards the lysis of M. luteus. Against this substrate, it was three times more efficient than HEW-lysozyme. It also cleaved E. coli B, but its efficiency was half as much as with M. luteus. However, it cleaved P. septica and B. subtilis at a rate similar to HEW-lysozyme under identical conditions.", "contents": "Structure-activity studies on mammalian tissue lytic enzymes: chemical characterization and substrate specificity of rat kidney nuclear lysozyme. Studies on the structure and substrate specificity of purified rat kidney nuclear (RKN) lysozyme are reported. The carboxyl and amino terminal residues of RKN-lysozyme were found to be leucine and alanine respectively. The amino acid composition indicated similarities and differences as compared with that of hen egg white (HEW) lysozyme. There were alterations in the nine amino acid residues, Lys, His, Arg, Asp, Glu, Pro, 1/2 Cys, Tyr and Trp. The other nine residues were present in identical proportions to those of HEW-lysozyme. The decrease in the arginine and aspartic acid residues was found to be compensated by the increase in the number of lysine, histidine and glutamic acid residues. The overall ratio of the acidic to basic amino acids has thus been conserved in the mammalian enzyme. In addition, RKN-lysozyme contained decreased numbers of Trp, Tyr and 1/2 Cys, and increased numbers of proline residues as found in HEW-lysozyme. RKN-lysozyme did not cross react with heterologous antibodies produced against HEW-lysozyme, and vice versa. RKN-lysozyme showed distinct specificity towards the lysis of M. luteus. Against this substrate, it was three times more efficient than HEW-lysozyme. It also cleaved E. coli B, but its efficiency was half as much as with M. luteus. However, it cleaved P. septica and B. subtilis at a rate similar to HEW-lysozyme under identical conditions.", "PMID": 955782}
{"id": "pubmed23n0032_1794", "title": "Polypeptide models of collagen: synthesis of (Pro-Pro-Ala)n and (Pro-Pro-Val)n.", "content": "The synthesis of two collagen models (Pro-Pro-Ala)n and (Pro-Pro-Val)n is reported. Preliminary examination suggests that (Pro-Pro-Ala)n may exhibit some properties of collagen-like polypeptides whereas (Pro-Pro-Val)n, does not have a collagen-like character.", "contents": "Polypeptide models of collagen: synthesis of (Pro-Pro-Ala)n and (Pro-Pro-Val)n. The synthesis of two collagen models (Pro-Pro-Ala)n and (Pro-Pro-Val)n is reported. Preliminary examination suggests that (Pro-Pro-Ala)n may exhibit some properties of collagen-like polypeptides whereas (Pro-Pro-Val)n, does not have a collagen-like character.", "PMID": 955783}
{"id": "pubmed23n0032_1795", "title": "Ego functioning during paranoid regression.", "content": "This paper is based on the psychotherapeutic work with a paranoid schizophrenic man during a period of acute psychotic regression lasting approximately two weeks. The author reviews what seemed to be the precipitating factors in the decompensation, then elaborates descriptively and speculatively on two major aspects of ego functioning during the regression, namely, the experiences of fragmentation and of dedifferentiation. The role that seemed to evolve for the therapist is also discussed as it relates to the aforementioned aspects of ego functioning. Finally, a tentative hypothesis is developed concerning pretense as one possible genetic factor in these ego disturbances.", "contents": "Ego functioning during paranoid regression. This paper is based on the psychotherapeutic work with a paranoid schizophrenic man during a period of acute psychotic regression lasting approximately two weeks. The author reviews what seemed to be the precipitating factors in the decompensation, then elaborates descriptively and speculatively on two major aspects of ego functioning during the regression, namely, the experiences of fragmentation and of dedifferentiation. The role that seemed to evolve for the therapist is also discussed as it relates to the aforementioned aspects of ego functioning. Finally, a tentative hypothesis is developed concerning pretense as one possible genetic factor in these ego disturbances.", "PMID": 955784}
{"id": "pubmed23n0032_1796", "title": "Victim, victimizer: interaction in the psychotherapy of borderline patients.", "content": "Borderline and narcissistic patients suffuse relationships with intense pregenital aggression to which the therapist, despite his level of training or self-understanding, is not totally immune. Aggression is considered an energetic expression of the narcissistic defense against vulnerability. The therapist is subject to a continuing siege of projections and projective identifications. Vigilance against repetitive sadomasochistic interactions is a necessary and often wearying extension of therapeutic concern. Most necessary, too, is the willingness to make second efforts at understanding the patient's vulnerability. Such understanding comes from attentiveness to countertransference feelings.", "contents": "Victim, victimizer: interaction in the psychotherapy of borderline patients. Borderline and narcissistic patients suffuse relationships with intense pregenital aggression to which the therapist, despite his level of training or self-understanding, is not totally immune. Aggression is considered an energetic expression of the narcissistic defense against vulnerability. The therapist is subject to a continuing siege of projections and projective identifications. Vigilance against repetitive sadomasochistic interactions is a necessary and often wearying extension of therapeutic concern. Most necessary, too, is the willingness to make second efforts at understanding the patient's vulnerability. Such understanding comes from attentiveness to countertransference feelings.", "PMID": 955785}
{"id": "pubmed23n0032_1797", "title": "The self as a transitional object: its relationship to narcissism and homosexuality.", "content": "Men with significant narcissistic and homosexual trends can show a tendency to experience a part of the self as a transitional object. Specifically, these men regard themselves as dolls. Excerpts from three psychoanalytic cases are offered to demonstrate this clinical finding. It is believed that this occurrence represents a significant arrest in the development of the self. The recognition, interpretation, and reduction of this transitional phenomenon constitutes a significant therapeutic experience in the analyses of these men.", "contents": "The self as a transitional object: its relationship to narcissism and homosexuality. Men with significant narcissistic and homosexual trends can show a tendency to experience a part of the self as a transitional object. Specifically, these men regard themselves as dolls. Excerpts from three psychoanalytic cases are offered to demonstrate this clinical finding. It is believed that this occurrence represents a significant arrest in the development of the self. The recognition, interpretation, and reduction of this transitional phenomenon constitutes a significant therapeutic experience in the analyses of these men.", "PMID": 955786}
{"id": "pubmed23n0032_1798", "title": "Transitional phenomena and therapeutic symbiosis.", "content": "This paper suggests that the analyst (or therapist) can best understand transitional-object phenomena as being tributary to, or consisting in various different facets of, the--for him in his work with patients--more comprehensive realm of therapeutic symobiosis. The author highlights data concerning objects or phenomena which are transitional for both patient and analyst concomitantly. He suggests that the patient's symptoms have become, early in the phase of therapeutic symbiosis, transitional objects for both patient and analyst simultaneously. As with the patient's symptoms, so with his transference images of the analyst: it is suggested that, in order for any effective transference analysis to occur with any patient, whether neurotic, borderline, or psychotic, the analyst must have come to accept at least a transitional-object degree--if not more deeply symbiotic degree--of relatedness with the particular transference image which is holding sway presently in the analysis.", "contents": "Transitional phenomena and therapeutic symbiosis. This paper suggests that the analyst (or therapist) can best understand transitional-object phenomena as being tributary to, or consisting in various different facets of, the--for him in his work with patients--more comprehensive realm of therapeutic symobiosis. The author highlights data concerning objects or phenomena which are transitional for both patient and analyst concomitantly. He suggests that the patient's symptoms have become, early in the phase of therapeutic symbiosis, transitional objects for both patient and analyst simultaneously. As with the patient's symptoms, so with his transference images of the analyst: it is suggested that, in order for any effective transference analysis to occur with any patient, whether neurotic, borderline, or psychotic, the analyst must have come to accept at least a transitional-object degree--if not more deeply symbiotic degree--of relatedness with the particular transference image which is holding sway presently in the analysis.", "PMID": 955787}
{"id": "pubmed23n0032_1799", "title": "Object choice and actual bisexuality.", "content": "Actual bisexuality is to be distinguished from homosexuality in a latent state and from conscious bisexual fantasies. Contemporary social changes have caused an increased demand for help for those men and women capable of engaging in protracted heterosexual and homosexual relations. Among such people narcissistic and borderline states are common. Clinical material is presented in some detail. The author suggests that the condition is associated with a tendency to be caught up between the anaclitic and narcissistic types of object choice. The concurrent involvement with a male and female love object against a background of pseudogenitality creates the illusory appearance of two objects being involved, covering up the fact that there is splitting of the original love object together with severe preoedipal disturbance.", "contents": "Object choice and actual bisexuality. Actual bisexuality is to be distinguished from homosexuality in a latent state and from conscious bisexual fantasies. Contemporary social changes have caused an increased demand for help for those men and women capable of engaging in protracted heterosexual and homosexual relations. Among such people narcissistic and borderline states are common. Clinical material is presented in some detail. The author suggests that the condition is associated with a tendency to be caught up between the anaclitic and narcissistic types of object choice. The concurrent involvement with a male and female love object against a background of pseudogenitality creates the illusory appearance of two objects being involved, covering up the fact that there is splitting of the original love object together with severe preoedipal disturbance.", "PMID": 955788}
{"id": "pubmed23n0032_1800", "title": "Transvertism: a disorder of the sense of self.", "content": "Transvestism is not simply a sexual disorder, but is best understood as primarily a disorder of the sense of self. A descriptive background of transvestism is provided through a review of developmental history, clinical course, presonality structure, and family history. The predominant transvestic fantasies and their modes of enactment are described. The disorder of the sence of self that gives rise to transvestic behavior is identified as a split in the ego into incompatible male and female gender identities. The split is attributed to an early identification with the mother as a defense against unresolved separation anxiety engendered during the separation-individuation phase of infantile development. The effect of this split upon the sense of reality, object relations, and adaptation to stress in transvestism are examined for a psychodynamic point of view.", "contents": "Transvertism: a disorder of the sense of self. Transvestism is not simply a sexual disorder, but is best understood as primarily a disorder of the sense of self. A descriptive background of transvestism is provided through a review of developmental history, clinical course, presonality structure, and family history. The predominant transvestic fantasies and their modes of enactment are described. The disorder of the sence of self that gives rise to transvestic behavior is identified as a split in the ego into incompatible male and female gender identities. The split is attributed to an early identification with the mother as a defense against unresolved separation anxiety engendered during the separation-individuation phase of infantile development. The effect of this split upon the sense of reality, object relations, and adaptation to stress in transvestism are examined for a psychodynamic point of view.", "PMID": 955789}
{"id": "pubmed23n0032_1801", "title": "Empathy and intuition in becoming a psychiatrist: a case study.", "content": "This paper, through the use of the case study, presents the idea that the beginning psychiatrist must often depend on his own native intuition and emphathic skills during his early clinical work. A case is presented in detail to show that amide the anxiety, freshness, and inexperience typifying a beginning psychiatrist, a psychotherapeutic treatment that benefits both the patient and the psychiatrist can be carried out. The growth of the patient and the therapist is described over the course of a three-month hospitalization.", "contents": "Empathy and intuition in becoming a psychiatrist: a case study. This paper, through the use of the case study, presents the idea that the beginning psychiatrist must often depend on his own native intuition and emphathic skills during his early clinical work. A case is presented in detail to show that amide the anxiety, freshness, and inexperience typifying a beginning psychiatrist, a psychotherapeutic treatment that benefits both the patient and the psychiatrist can be carried out. The growth of the patient and the therapist is described over the course of a three-month hospitalization.", "PMID": 955790}
{"id": "pubmed23n0032_1802", "title": "On becomine a psychiatrist: discussion of \"Empathy and intuition in becoming a psychiatrist,\" by Ronald J. Blank.", "content": "Using the data from Blank's (1976) description of his clinical efforts with his first patient, selected tissues on becoming a psychiatrist and psychotherapist are explored. Considered among the motives for entering this profession are opportunities for the therapist to projectively identify into his patients, and to introjectively identify with and contain his patients' psychopathology. The relationship between empathy and intuition on the one hand, and projection and projective identification on the other, is also studied, as is the need for the application of the validating process in confirming all so-called empathic and intuitive responses on the part of the therapist. Counter-transference influences on the experience and use of empathy and intuition are also investigated. The development of therapeutic misalliances and framework \"cures,\" the distinction between transference and nontransference, the constructive elements contained in essentially countertransference-based interventions, the mastery of countertransference difficulties, and the choice of insight-oriented versus noninsightful therapeutic modalities are discussed.", "contents": "On becomine a psychiatrist: discussion of \"Empathy and intuition in becoming a psychiatrist,\" by Ronald J. Blank. Using the data from Blank's (1976) description of his clinical efforts with his first patient, selected tissues on becoming a psychiatrist and psychotherapist are explored. Considered among the motives for entering this profession are opportunities for the therapist to projectively identify into his patients, and to introjectively identify with and contain his patients' psychopathology. The relationship between empathy and intuition on the one hand, and projection and projective identification on the other, is also studied, as is the need for the application of the validating process in confirming all so-called empathic and intuitive responses on the part of the therapist. Counter-transference influences on the experience and use of empathy and intuition are also investigated. The development of therapeutic misalliances and framework \"cures,\" the distinction between transference and nontransference, the constructive elements contained in essentially countertransference-based interventions, the mastery of countertransference difficulties, and the choice of insight-oriented versus noninsightful therapeutic modalities are discussed.", "PMID": 955791}
{"id": "pubmed23n0032_1803", "title": "Toward a general concept of the therapeutic process.", "content": "Using clinical psychoanalytic theory as a unifying concept, an attempt is made to observe, interpret, and integrate the therapeutic process in a wide variety of psychotherapies. All psychotherapy, regardless of specific form or technique, is viewed as an interpersonal or intrapersonal process, and should be understandable from a psychoanalytic perspective. Ten common factors are selected: structure of the therapeutic situation; the therapeutic relationship; management of anxiety; drives and their derivatives; mechanisms of defense; identification; regression; catharsis and abbreaction; external reinforcement; structural change. The various psychotherapies are compared regarding each of the ten factors.", "contents": "Toward a general concept of the therapeutic process. Using clinical psychoanalytic theory as a unifying concept, an attempt is made to observe, interpret, and integrate the therapeutic process in a wide variety of psychotherapies. All psychotherapy, regardless of specific form or technique, is viewed as an interpersonal or intrapersonal process, and should be understandable from a psychoanalytic perspective. Ten common factors are selected: structure of the therapeutic situation; the therapeutic relationship; management of anxiety; drives and their derivatives; mechanisms of defense; identification; regression; catharsis and abbreaction; external reinforcement; structural change. The various psychotherapies are compared regarding each of the ten factors.", "PMID": 955792}
{"id": "pubmed23n0032_1804", "title": "Psychotherapists' passivity--a major training problem.", "content": "A major problem encountered in the early psychotherapy training of psychiatric residents, psychologists, and social workers is the tendency for the student to take an inappropriately passive and silent role. Rather than actively engage patients in a therapeutic alliance, beginning students have the tendency to remain withdrawn and inarticulate. This often reaches extreme proportions and may later significantly block treatment progress. Clinical vignettes are presented to show the range and variety of hyperpassive behavior in psychotherapy training and the potential crippling and destructive effects on the treatment process. This is not to imply that there is no danger in excess activity on the part of the therapist. Indeed, not all silence need be destructive. However, detachment, withdrawal, and excess silence--attitudes often fostered by our training programs--can be markedly destructive to both patients and treatment. Psychodynamic factors causing this situation are reviewed. A variety of methods and strategies are recommended for dealing with it. If the supervisor remains alert, the problem can be significantly resolved before it interferes with treatment. In this way, the student will be helped to overcome one of the major ealry hurdles of training.", "contents": "Psychotherapists' passivity--a major training problem. A major problem encountered in the early psychotherapy training of psychiatric residents, psychologists, and social workers is the tendency for the student to take an inappropriately passive and silent role. Rather than actively engage patients in a therapeutic alliance, beginning students have the tendency to remain withdrawn and inarticulate. This often reaches extreme proportions and may later significantly block treatment progress. Clinical vignettes are presented to show the range and variety of hyperpassive behavior in psychotherapy training and the potential crippling and destructive effects on the treatment process. This is not to imply that there is no danger in excess activity on the part of the therapist. Indeed, not all silence need be destructive. However, detachment, withdrawal, and excess silence--attitudes often fostered by our training programs--can be markedly destructive to both patients and treatment. Psychodynamic factors causing this situation are reviewed. A variety of methods and strategies are recommended for dealing with it. If the supervisor remains alert, the problem can be significantly resolved before it interferes with treatment. In this way, the student will be helped to overcome one of the major ealry hurdles of training.", "PMID": 955793}
{"id": "pubmed23n0032_1805", "title": "The Ellsberg psychoanalytic situation.", "content": "The author proposes to explore an event that received nationwide publicity through the Watergate investigation: the burglary of the office of a Los Angeles psychoanalyst believed to be treating Dr. Daniel Ellsberg. A brief questionnaire, with an accompanying letter, was mailed to all members of the American Psychoanalytic Association. It requested voluntary information on the reaction of their patients to the aforesaid particular event. The results of the study was then evaluated as serving hopefully an indirect inquiry into psychoanalytic practice. This paper is based on an adjusted sample of 861 respondent psychoanalysts, who reported on a total of 5,074 analysands. The resultant figures indicate that a high percentage of the analysand sample was silent concerning the office break-in during the eight-week period following the national publicity given the event. Of the small percentage of analysands that did present material concerning the event, the survey found that they were the patients of only 84 analysts of the entire sample of 861. The absence of reference to the Ellsberg Affair in so many patients may have been due to (1) the fact that analysts create an analytic situation which has a basic quality of interference such that events of this kind do not get communicated, and (2), more important, the fact that analysts through some defensive need failed to take cognizance of references to the Ellsberg Affair in dreams or in the latent content of the associations of their patients. A \"natural\" psychoanalytic experiment has been studied, and the results are offered for consideration.", "contents": "The Ellsberg psychoanalytic situation. The author proposes to explore an event that received nationwide publicity through the Watergate investigation: the burglary of the office of a Los Angeles psychoanalyst believed to be treating Dr. Daniel Ellsberg. A brief questionnaire, with an accompanying letter, was mailed to all members of the American Psychoanalytic Association. It requested voluntary information on the reaction of their patients to the aforesaid particular event. The results of the study was then evaluated as serving hopefully an indirect inquiry into psychoanalytic practice. This paper is based on an adjusted sample of 861 respondent psychoanalysts, who reported on a total of 5,074 analysands. The resultant figures indicate that a high percentage of the analysand sample was silent concerning the office break-in during the eight-week period following the national publicity given the event. Of the small percentage of analysands that did present material concerning the event, the survey found that they were the patients of only 84 analysts of the entire sample of 861. The absence of reference to the Ellsberg Affair in so many patients may have been due to (1) the fact that analysts create an analytic situation which has a basic quality of interference such that events of this kind do not get communicated, and (2), more important, the fact that analysts through some defensive need failed to take cognizance of references to the Ellsberg Affair in dreams or in the latent content of the associations of their patients. A \"natural\" psychoanalytic experiment has been studied, and the results are offered for consideration.", "PMID": 955794}
{"id": "pubmed23n0032_1806", "title": "Conservatism and liberalism: a psychoanalytic examination of political belief.", "content": "A review of the historical development of conservative and liberal through reveals contradictory basic assumptions: the former views man as essentially evil by nature, and places its priorities upon self-restraint; the latter views man as essentially good, and with umlimited capabilities, and celebrates his efforts at creating a perfect society for himself. Three clinical vignettes are recounted in which the patient's intrapyschic organization found support from a hormonious political philosophy. It is suggested that conservatism and liberalism offer the individual an ideological resolution to the narcissistic injuries he experiences as a consequence of life's inevitable and developmentally determined disappointments.", "contents": "Conservatism and liberalism: a psychoanalytic examination of political belief. A review of the historical development of conservative and liberal through reveals contradictory basic assumptions: the former views man as essentially evil by nature, and places its priorities upon self-restraint; the latter views man as essentially good, and with umlimited capabilities, and celebrates his efforts at creating a perfect society for himself. Three clinical vignettes are recounted in which the patient's intrapyschic organization found support from a hormonious political philosophy. It is suggested that conservatism and liberalism offer the individual an ideological resolution to the narcissistic injuries he experiences as a consequence of life's inevitable and developmentally determined disappointments.", "PMID": 955795}
{"id": "pubmed23n0032_1807", "title": "Homogamous and heterogamous marriages.", "content": "This paper explores two marital constructs set forth by Peter Giovacchini. The first is based on a symbiotic reenactment in which the investment of both partners is tenacious and reciprocal, an attachment which Giovacchini refers to as a \"character object\" relationship. It represents intrapsychic homogramy. In the second construct, this deep form of attraction is absent and the dyadic bond is superficial, frequently transitory. The latter couples do not require an elementary, intrapsychic bond but do share particular defensive traits or symptoms. Thus these unions are termed \"symptom object\" relationships and are characterologically heterogamous. By means of a group case study approach, this distinction was statistically supported. Furthermore, it was found that homogamous marriages stay in treatment longer, maintain a lower divorce rate, and respond best to individual psychoanalytic psychotherapy. Heterogamous marriages, by contrast, show a higher divorce rate and tend to leave therapy prior to termination. The research also suggests that the initial treatment of choice for this type of marriage may well be conjoint or group psychotherapy.", "contents": "Homogamous and heterogamous marriages. This paper explores two marital constructs set forth by Peter Giovacchini. The first is based on a symbiotic reenactment in which the investment of both partners is tenacious and reciprocal, an attachment which Giovacchini refers to as a \"character object\" relationship. It represents intrapsychic homogramy. In the second construct, this deep form of attraction is absent and the dyadic bond is superficial, frequently transitory. The latter couples do not require an elementary, intrapsychic bond but do share particular defensive traits or symptoms. Thus these unions are termed \"symptom object\" relationships and are characterologically heterogamous. By means of a group case study approach, this distinction was statistically supported. Furthermore, it was found that homogamous marriages stay in treatment longer, maintain a lower divorce rate, and respond best to individual psychoanalytic psychotherapy. Heterogamous marriages, by contrast, show a higher divorce rate and tend to leave therapy prior to termination. The research also suggests that the initial treatment of choice for this type of marriage may well be conjoint or group psychotherapy.", "PMID": 955796}
{"id": "pubmed23n0032_1808", "title": "Symbiosis and intimacy.", "content": "The symbiotic phase of development is crucially involved in determing the nature of psychopathology as well as promoting psychic processes that are involved in creative activity and intimate object relationships. In a well-established object relationship, the partners relate to each other in a symbiotic fashion. From the analysis of married persons (and some clinical material is presented in this paper), the author concludes that the fundamental character structure, psychopathological or otherwise, of each spouse is identical. Exceptions to this conclusion exist, but here one is dealing with an object relationship that is superficial and transitory. Symbiotic fusion regularly occurs in creative activity and empathic intimate relationships.", "contents": "Symbiosis and intimacy. The symbiotic phase of development is crucially involved in determing the nature of psychopathology as well as promoting psychic processes that are involved in creative activity and intimate object relationships. In a well-established object relationship, the partners relate to each other in a symbiotic fashion. From the analysis of married persons (and some clinical material is presented in this paper), the author concludes that the fundamental character structure, psychopathological or otherwise, of each spouse is identical. Exceptions to this conclusion exist, but here one is dealing with an object relationship that is superficial and transitory. Symbiotic fusion regularly occurs in creative activity and empathic intimate relationships.", "PMID": 955797}
{"id": "pubmed23n0032_1809", "title": "Psychological unevenness in the academically successful student.", "content": "Among students seen is psychoanalytic psychotherapy, there is a group who present the paradoxical clinical picture of great academic success alongside of quite primitive features in other aspects of their psychological functioning. This article offers an early developmental formulation of this aspect of these students. A clinical description of these patients is presented, including the observation that for each the early mother-child relationship seems to have been characterized by a predominant focus on maternal needs. A case history and summary of therapy of one of these students is discussed. Aspects of the mother-child relationship are explored by means of historical data, memories, and most important, through various transference and countertransference manifestations. The nature of this early relationship is then discussed in terms of the work of Donald Winnicott and Masud Khan. Finally, an overview of therapy is presented along with a discussion of some forms of resistance and some countertransference problems that often arise in the course of therapy with such students.", "contents": "Psychological unevenness in the academically successful student. Among students seen is psychoanalytic psychotherapy, there is a group who present the paradoxical clinical picture of great academic success alongside of quite primitive features in other aspects of their psychological functioning. This article offers an early developmental formulation of this aspect of these students. A clinical description of these patients is presented, including the observation that for each the early mother-child relationship seems to have been characterized by a predominant focus on maternal needs. A case history and summary of therapy of one of these students is discussed. Aspects of the mother-child relationship are explored by means of historical data, memories, and most important, through various transference and countertransference manifestations. The nature of this early relationship is then discussed in terms of the work of Donald Winnicott and Masud Khan. Finally, an overview of therapy is presented along with a discussion of some forms of resistance and some countertransference problems that often arise in the course of therapy with such students.", "PMID": 955798}
{"id": "pubmed23n0032_1810", "title": "Alan Strange as an adolescent: a discussion of Peter Shaffer's Equus.", "content": "Psychoanalytic knowledge of adolescence helps us to understand the protagonists of Peter Shaffer's play Equus. Alan Strang, the seventeen-year-old who maimed six horses, had undergone a retreat from oedipal wishes to narcissism typical of teenagers. His fragile attempts at achieving a sound sense of identity are undermined when his seeking of a noninestuous object produces panic and impotence. Dr. Dysart, the psychiatrist who envies his patient's passion, and fears hurting him through treatment, displays in an exaggerated way countertransferences often seen in therapists of adolescents. In addition, his doubts have some foundation in that the treatment he employs, catharsis, can encourage primitive defenses and the outpouring of uncontrolled, unneutralized drive derivatives. This is more likely to prevent adaptive creativity than the cure the doctor dreads. Finally, some technical problems in dealing with sublimations in psychoanalysis (which is not Dr. Dysart's form of treatment) are discussed.", "contents": "Alan Strange as an adolescent: a discussion of Peter Shaffer's Equus. Psychoanalytic knowledge of adolescence helps us to understand the protagonists of Peter Shaffer's play Equus. Alan Strang, the seventeen-year-old who maimed six horses, had undergone a retreat from oedipal wishes to narcissism typical of teenagers. His fragile attempts at achieving a sound sense of identity are undermined when his seeking of a noninestuous object produces panic and impotence. Dr. Dysart, the psychiatrist who envies his patient's passion, and fears hurting him through treatment, displays in an exaggerated way countertransferences often seen in therapists of adolescents. In addition, his doubts have some foundation in that the treatment he employs, catharsis, can encourage primitive defenses and the outpouring of uncontrolled, unneutralized drive derivatives. This is more likely to prevent adaptive creativity than the cure the doctor dreads. Finally, some technical problems in dealing with sublimations in psychoanalysis (which is not Dr. Dysart's form of treatment) are discussed.", "PMID": 955799}
{"id": "pubmed23n0032_1811", "title": "Equus and the psychopathology of passion.", "content": "Peter Shaffer's play Equus is discussed in terms of its views on the concept of passion. The major characters show clear indications of psychopathological expressions of passion. Alan's pathology is highlighted by his psychotic identification with Equus and his binding of the horses. Dr. Dysart's pathology is seen in his greatly inhibited sense of passion. He envies Alan's passion and shows \"countertransference\" toward treating him. Both characters are studied in terms of level of object relations, ego functions, and unconscious fantasy.", "contents": "Equus and the psychopathology of passion. Peter Shaffer's play Equus is discussed in terms of its views on the concept of passion. The major characters show clear indications of psychopathological expressions of passion. Alan's pathology is highlighted by his psychotic identification with Equus and his binding of the horses. Dr. Dysart's pathology is seen in his greatly inhibited sense of passion. He envies Alan's passion and shows \"countertransference\" toward treating him. Both characters are studied in terms of level of object relations, ego functions, and unconscious fantasy.", "PMID": 955800}
{"id": "pubmed23n0032_1812", "title": "Discussion of papers on Equus.", "content": "Three points are discussed concerning Equus and the preceding papers: (1) Because a play is not a free association obtained within the context of the analytic situation it is most difficult to \"analyze.\" (2) The central theme of \"passion\" in Equus would seem to relate to the vicissitudes of infantile omnipotence, as noted in both the content of the play and the process of playwrighting. And (3) in Equus, the playwright not only creates a reality out of a fantasy, but \"tricks\" the audience into being a participant in the scopophilic activities depicted.", "contents": "Discussion of papers on Equus. Three points are discussed concerning Equus and the preceding papers: (1) Because a play is not a free association obtained within the context of the analytic situation it is most difficult to \"analyze.\" (2) The central theme of \"passion\" in Equus would seem to relate to the vicissitudes of infantile omnipotence, as noted in both the content of the play and the process of playwrighting. And (3) in Equus, the playwright not only creates a reality out of a fantasy, but \"tricks\" the audience into being a participant in the scopophilic activities depicted.", "PMID": 955801}
{"id": "pubmed23n0032_1813", "title": "Intersecting languages in psychoanalysis and philosophy.", "content": "The introduction establishes a general view of the literature in which philosophers have profited from their encounters with analysis. It provides a frame within which to present more specific ideas about the method and language of psychoanalysis as viewed by philosophers. The method of interpretive reconstruction is unfolded from its original context of Freud's archaeological analogy. Further, the vocabulary of reconstruction, which is an intimate part of this analogy, is employed by Anna Freud is her discussion of defense mechanisms. Texts are cited and explicated. Meanwhile, the method of reconstruction is given independent, though related, application in the work of R. G. Collingwood, an archaeologist-philosopher-historian. The juxtaposition of Freud and Collingwood suggests that the methods of philosophy and analysis are more alike than the particular problems they try to solve. Both methods are oriented toward solving the problem of discovering meaning amid absurdity. The introduction of two specific examples lends substances to this claim. In the final section on the practice of interpretation, the question is raised as to how the introduction of the method of reconstruction affects the debate about the epistemological status of psychoanalysis as a science. Psychoanalytic knowledge shows itself to be more like that available to the historian than that accessible through physical theories. Still, physics and analysis can be compared. One must look to the interpretation of symbols. In psychoanalysis, giving an interpretation--in which nonsense becomes understandable--is a form of explanation. This methodological result suggests a conclusion about the relation between metapsychology and clinical practice.", "contents": "Intersecting languages in psychoanalysis and philosophy. The introduction establishes a general view of the literature in which philosophers have profited from their encounters with analysis. It provides a frame within which to present more specific ideas about the method and language of psychoanalysis as viewed by philosophers. The method of interpretive reconstruction is unfolded from its original context of Freud's archaeological analogy. Further, the vocabulary of reconstruction, which is an intimate part of this analogy, is employed by Anna Freud is her discussion of defense mechanisms. Texts are cited and explicated. Meanwhile, the method of reconstruction is given independent, though related, application in the work of R. G. Collingwood, an archaeologist-philosopher-historian. The juxtaposition of Freud and Collingwood suggests that the methods of philosophy and analysis are more alike than the particular problems they try to solve. Both methods are oriented toward solving the problem of discovering meaning amid absurdity. The introduction of two specific examples lends substances to this claim. In the final section on the practice of interpretation, the question is raised as to how the introduction of the method of reconstruction affects the debate about the epistemological status of psychoanalysis as a science. Psychoanalytic knowledge shows itself to be more like that available to the historian than that accessible through physical theories. Still, physics and analysis can be compared. One must look to the interpretation of symbols. In psychoanalysis, giving an interpretation--in which nonsense becomes understandable--is a form of explanation. This methodological result suggests a conclusion about the relation between metapsychology and clinical practice.", "PMID": 955802}
{"id": "pubmed23n0032_1814", "title": "Sartre's contribution to the understanding of narcissism.", "content": "As a means for presenting Sartre's insights into the narcissistic problems of the self, we have used his phenomenological system as articulated in Being and Nothingness (1943) to illuminate these issues in the personality of Roquentin, the hero of his novel Nausea (1938). Roquentin attempts to stabilize his fragmenting self and to avoid \"nausea\" by using three mechanisms which Sartre argues maintain the self from drowning in the objects of the self. These are \"reflection,\" \"temporality\" (continuity through time), and \"being-for-others\" (how we experience another's view of ourselves). In Sartre's conception of being-for-others lies many clinically useful insights which can be used to explain both the structure and the instability of the transferences seen in the treatment of narcissistic personality disorders. Sartre demonstrates by implication that the patient must maintain (by using bad faith, i.e., disavowal) that the therapist is acting freely, or these transferences collapse. Thus, the patient must feel he is the unique and special \"occasion,\" of any warmth, empathy, or compliments. A case example is included to illustrate these issues of freedom and bad faith.", "contents": "Sartre's contribution to the understanding of narcissism. As a means for presenting Sartre's insights into the narcissistic problems of the self, we have used his phenomenological system as articulated in Being and Nothingness (1943) to illuminate these issues in the personality of Roquentin, the hero of his novel Nausea (1938). Roquentin attempts to stabilize his fragmenting self and to avoid \"nausea\" by using three mechanisms which Sartre argues maintain the self from drowning in the objects of the self. These are \"reflection,\" \"temporality\" (continuity through time), and \"being-for-others\" (how we experience another's view of ourselves). In Sartre's conception of being-for-others lies many clinically useful insights which can be used to explain both the structure and the instability of the transferences seen in the treatment of narcissistic personality disorders. Sartre demonstrates by implication that the patient must maintain (by using bad faith, i.e., disavowal) that the therapist is acting freely, or these transferences collapse. Thus, the patient must feel he is the unique and special \"occasion,\" of any warmth, empathy, or compliments. A case example is included to illustrate these issues of freedom and bad faith.", "PMID": 955803}
{"id": "pubmed23n0032_1815", "title": "The mirror transference in the psychoanalytic psychotherapy of alcoholism: a case report.", "content": "The development of a mirror transference in the successful outpatient psychoanalytic psychotherapy of an alcoholic patient is described in this paper. Important features of the case were a very severe initial period of resistance, a dramatic shift from a climate of bitterness and rancor to one of quiet and satisfaction that coincided with the establishment of the mirroring relationship, then recall of important genetic material, and finally, a period of many gains in the patient's contemporary relationships. Very few interpretations were given. The psychotherapy was chiefly an attempt to provide the object relationship that would be therapeutic, an archaic relationship of the grandiose self and the mirroring self-object. The theoretical work of Balint and Kohut was applied to this problem (with the alcoholic patient) and proved extremely helpful in predicting the major turning points in the psychotherapy and in guiding the strategy of the therapist. The principal difficulty not fully predicted was the extent and depth of the resistance to a positive transferace; once this resistance was overcome, the psychotherapy proceeded in a way similar to that generally described by Kohut with regard to patients who became involved in a mirror transference.", "contents": "The mirror transference in the psychoanalytic psychotherapy of alcoholism: a case report. The development of a mirror transference in the successful outpatient psychoanalytic psychotherapy of an alcoholic patient is described in this paper. Important features of the case were a very severe initial period of resistance, a dramatic shift from a climate of bitterness and rancor to one of quiet and satisfaction that coincided with the establishment of the mirroring relationship, then recall of important genetic material, and finally, a period of many gains in the patient's contemporary relationships. Very few interpretations were given. The psychotherapy was chiefly an attempt to provide the object relationship that would be therapeutic, an archaic relationship of the grandiose self and the mirroring self-object. The theoretical work of Balint and Kohut was applied to this problem (with the alcoholic patient) and proved extremely helpful in predicting the major turning points in the psychotherapy and in guiding the strategy of the therapist. The principal difficulty not fully predicted was the extent and depth of the resistance to a positive transferace; once this resistance was overcome, the psychotherapy proceeded in a way similar to that generally described by Kohut with regard to patients who became involved in a mirror transference.", "PMID": 955804}
{"id": "pubmed23n0032_1816", "title": "Psychotherapeutic schema based on the paranoid process.", "content": "A psychotherapeutic schema is proposed based on the analysis of the paranoid process. The paranoid process involves a paranoid construction of the patient's view of reality and his environment, particularly the social environment. The paranoid construction sustains and makes sense out of the patient's projective system. These projective elements reflect and are derived from the introjects--the internalized objects around which the patient's inner world and his experience of himself are organized. The schema aims at focusing the therapy on these introjects as central to the psychopathology. The following steps of the schema are outlined and discussed: establishing the therapeutic alliance, defining the projective system, testing reality, clarification of the introjects, derivation of the introjects, motivation of the introjects, mourning of infantile attachments, emergence of transference dependence, transference resolution, and termination.", "contents": "Psychotherapeutic schema based on the paranoid process. A psychotherapeutic schema is proposed based on the analysis of the paranoid process. The paranoid process involves a paranoid construction of the patient's view of reality and his environment, particularly the social environment. The paranoid construction sustains and makes sense out of the patient's projective system. These projective elements reflect and are derived from the introjects--the internalized objects around which the patient's inner world and his experience of himself are organized. The schema aims at focusing the therapy on these introjects as central to the psychopathology. The following steps of the schema are outlined and discussed: establishing the therapeutic alliance, defining the projective system, testing reality, clarification of the introjects, derivation of the introjects, motivation of the introjects, mourning of infantile attachments, emergence of transference dependence, transference resolution, and termination.", "PMID": 955805}
{"id": "pubmed23n0032_1817", "title": "Development of neurophysiological indices of retardation: interhemispheric asymmetry of the visual evoked cortical response.", "content": "Visual evoked cortical responses (VECRs) were recorded from the left (O1) and right (02) hemispheres at varying interstimulus intervals (ISIs) in children who were either: Normal (dextral), Dextral Retardates, or Sinistral Retardates. Latencies of N1 and P2 components of the VECR were stable and similar for both hemispheres in all groups. There was an asymmetry between the hemispheres for N1-P2 amplitude: right greater than left in Normals; this direction of asymmetry was also present in Sinistral Retardates, but was reversed in Dextral Retardates. The direction of normal VECR asymmetry was the reverse of that previously established for the auditory evoked cortical response (AECR) amplitude (Richlin, et al., 1973; Matsumiya, et al., 1972; Morrell & Salamy, 1971; Wood, et al., 1971). It is suggested that some neural mechanism of the retardates may allow excessive non-verbal stimulation of the normally \"verbal\" hemisphere. The electrical activity of the brain consequent to auditory or visual stimulation appears to depend upon: the modality of stimulation, the mental status of the individual, and his handedness. These factors have led to the establishment of an additional index to provide differential diagnosis of the mental status of the child.", "contents": "Development of neurophysiological indices of retardation: interhemispheric asymmetry of the visual evoked cortical response. Visual evoked cortical responses (VECRs) were recorded from the left (O1) and right (02) hemispheres at varying interstimulus intervals (ISIs) in children who were either: Normal (dextral), Dextral Retardates, or Sinistral Retardates. Latencies of N1 and P2 components of the VECR were stable and similar for both hemispheres in all groups. There was an asymmetry between the hemispheres for N1-P2 amplitude: right greater than left in Normals; this direction of asymmetry was also present in Sinistral Retardates, but was reversed in Dextral Retardates. The direction of normal VECR asymmetry was the reverse of that previously established for the auditory evoked cortical response (AECR) amplitude (Richlin, et al., 1973; Matsumiya, et al., 1972; Morrell & Salamy, 1971; Wood, et al., 1971). It is suggested that some neural mechanism of the retardates may allow excessive non-verbal stimulation of the normally \"verbal\" hemisphere. The electrical activity of the brain consequent to auditory or visual stimulation appears to depend upon: the modality of stimulation, the mental status of the individual, and his handedness. These factors have led to the establishment of an additional index to provide differential diagnosis of the mental status of the child.", "PMID": 955806}
{"id": "pubmed23n0032_1818", "title": "Levels of norepinephrine in rat cerebellum after administration of the convulsant 3-mercaptopropionic acid.", "content": "The level of norepinephrine in rat cerebellum was assayed after the administration of the convulsant 3-mercaptopropionic acid. Decrease in norepinephrine was observed after the onset of seizure. The results were compared with those of GABA which were diminished both during and after convulsions. The decrease of GABA was prior to that of norepinephrine.", "contents": "Levels of norepinephrine in rat cerebellum after administration of the convulsant 3-mercaptopropionic acid. The level of norepinephrine in rat cerebellum was assayed after the administration of the convulsant 3-mercaptopropionic acid. Decrease in norepinephrine was observed after the onset of seizure. The results were compared with those of GABA which were diminished both during and after convulsions. The decrease of GABA was prior to that of norepinephrine.", "PMID": 955807}
{"id": "pubmed23n0032_1819", "title": "The differential effects of semantic, acoustic, and nonverbal distraction on Korsakoff patients' verbal retention performance.", "content": "Korsakoff and alcoholic control patients' verbal retention performance was assessed following either acoustic, semantic, or nonverbal distractor activity. Both patient groups were similarly affected by the degree of relatedness between the distractor and the memoranda. Semantic distraction produced more verbal forgetting than acoustic, which in turn, caused more forgetting than the nonverbal tasks. The Korsakoff patients' verbal retention, however, became increasingly impaired, relative to the controls, as the level of similarity between the distraction and the memoranda increased. These results suggest that Korsakoff patients have  difficulty performing verbal analyses which incorporated the distinctive physical, phonemic and semantic features of the memoranda into the development of their verbal memory traces.", "contents": "The differential effects of semantic, acoustic, and nonverbal distraction on Korsakoff patients' verbal retention performance. Korsakoff and alcoholic control patients' verbal retention performance was assessed following either acoustic, semantic, or nonverbal distractor activity. Both patient groups were similarly affected by the degree of relatedness between the distractor and the memoranda. Semantic distraction produced more verbal forgetting than acoustic, which in turn, caused more forgetting than the nonverbal tasks. The Korsakoff patients' verbal retention, however, became increasingly impaired, relative to the controls, as the level of similarity between the distraction and the memoranda increased. These results suggest that Korsakoff patients have  difficulty performing verbal analyses which incorporated the distinctive physical, phonemic and semantic features of the memoranda into the development of their verbal memory traces.", "PMID": 955808}
{"id": "pubmed23n0032_1820", "title": "A neuropsychodiagnostic model.", "content": "Central Nervous System pathology in children is regarded as a neurophysiological and/or neuroanatomical disorder leading to the production of a broad spectrum of symptomatic indicators, many of which are psychological in nature. This fundamental relationship between neurological integrity and various categories of psychopathology represents the theoretical core of a specific diagnostic rationale elaborated in the context of a neuropsychodiagnostic model. A battery of psychological tests, as one component of a more general assessment approach, is proposed as a psychoeducational method complementing the more traditional medical approach to the diagnosis of various neuropathological conditions in children, especially minimal brain damage/dysfunction.", "contents": "A neuropsychodiagnostic model. Central Nervous System pathology in children is regarded as a neurophysiological and/or neuroanatomical disorder leading to the production of a broad spectrum of symptomatic indicators, many of which are psychological in nature. This fundamental relationship between neurological integrity and various categories of psychopathology represents the theoretical core of a specific diagnostic rationale elaborated in the context of a neuropsychodiagnostic model. A battery of psychological tests, as one component of a more general assessment approach, is proposed as a psychoeducational method complementing the more traditional medical approach to the diagnosis of various neuropathological conditions in children, especially minimal brain damage/dysfunction.", "PMID": 955809}
{"id": "pubmed23n0032_1821", "title": "Fucosidosis: ultrastructural study of conjunctiva and skin and enzyme analysis of tears.", "content": "Conjunctival and skin biopsies from two new patients with fucosidosis were studied by electron microscopy. In both tissues, the connective tissue cells and the capillary endothelial cells were filled with single membrane limited inclusions of two types: (1) Clear inclusions containing a fibrillogranular reticulum. (2) Dark inclusions with a dense granular material. Specific stainings in ultrastructure suggest that these inclusions contain oligosaccharide chains. The ultrastructural aspect is characteristic for fucosidosis. Enzyme studies on tears realized an easy and secure technique for the diagnosis of the disease.", "contents": "Fucosidosis: ultrastructural study of conjunctiva and skin and enzyme analysis of tears. Conjunctival and skin biopsies from two new patients with fucosidosis were studied by electron microscopy. In both tissues, the connective tissue cells and the capillary endothelial cells were filled with single membrane limited inclusions of two types: (1) Clear inclusions containing a fibrillogranular reticulum. (2) Dark inclusions with a dense granular material. Specific stainings in ultrastructure suggest that these inclusions contain oligosaccharide chains. The ultrastructural aspect is characteristic for fucosidosis. Enzyme studies on tears realized an easy and secure technique for the diagnosis of the disease.", "PMID": 955828}
{"id": "pubmed23n0032_1822", "title": "Analog vs. digital photogrammetry for optic cup analysis.", "content": "This report compares the reproducibility of analog and digital photogrammetry for topographical analysis of the optic cup. Ten simultaneous stereophotographs of each of three optic discs were taken with the Zeiss fundus camera plus a twin-prism stereoseparator. Digital processing produced photogrammetric contours of the optic cup and estimates of its geometry. Optic cup contours from the same stereopairs were also drawn with an analog stereoplotter and were analyzed by manual planimetry. The reproducibility of digital photogrammetry and analysis for estimation of cup depth, cup width, cup cross sectional area, and cup volume was concluded to be generally superior to the reproducibility of analog photogrammetry and manual analysis.", "contents": "Analog vs. digital photogrammetry for optic cup analysis. This report compares the reproducibility of analog and digital photogrammetry for topographical analysis of the optic cup. Ten simultaneous stereophotographs of each of three optic discs were taken with the Zeiss fundus camera plus a twin-prism stereoseparator. Digital processing produced photogrammetric contours of the optic cup and estimates of its geometry. Optic cup contours from the same stereopairs were also drawn with an analog stereoplotter and were analyzed by manual planimetry. The reproducibility of digital photogrammetry and analysis for estimation of cup depth, cup width, cup cross sectional area, and cup volume was concluded to be generally superior to the reproducibility of analog photogrammetry and manual analysis.", "PMID": 955829}
{"id": "pubmed23n0032_1823", "title": "Morphological fiber types of retractor bulbi muscle in mouse and rat.", "content": "Retractor bulbi muscles of mouse and rat were examined by light and electron microscopy. Two morphological fiber types were observed, analogous to Type I and Type II cells of skeletal musculature and comparable to fibers observed in the global region of the rectus extraocular muscles of these species.", "contents": "Morphological fiber types of retractor bulbi muscle in mouse and rat. Retractor bulbi muscles of mouse and rat were examined by light and electron microscopy. Two morphological fiber types were observed, analogous to Type I and Type II cells of skeletal musculature and comparable to fibers observed in the global region of the rectus extraocular muscles of these species.", "PMID": 955830}
{"id": "pubmed23n0032_1824", "title": "Adaptive programming of phasic and tonic components in saccadic eye movements. Investigations of patients with abducens palsy.", "content": "Two patients with unilateral abducens palsy used their paretic-but visually superior-eye for fixation. In these patients, adaptive changes of saccadic amplitude and of the relation between phasic and tonic components of saccades were found. These changes led to a reduction of foveation time.", "contents": "Adaptive programming of phasic and tonic components in saccadic eye movements. Investigations of patients with abducens palsy. Two patients with unilateral abducens palsy used their paretic-but visually superior-eye for fixation. In these patients, adaptive changes of saccadic amplitude and of the relation between phasic and tonic components of saccades were found. These changes led to a reduction of foveation time.", "PMID": 955831}
{"id": "pubmed23n0032_1825", "title": "On the site of strabismic suppression.", "content": "A grating pattern presented to one eye of an individual capable of alternating suppression induces a visual aftereffect (contrast threshold elevation), even when the pattern is phenomenally suppressed from vision. This finding indicates that strabismic suppression occurs within the visual system after the site of the aftereffect, and points out a similarity between strabismic suppression and binocular rivalry suppression.", "contents": "On the site of strabismic suppression. A grating pattern presented to one eye of an individual capable of alternating suppression induces a visual aftereffect (contrast threshold elevation), even when the pattern is phenomenally suppressed from vision. This finding indicates that strabismic suppression occurs within the visual system after the site of the aftereffect, and points out a similarity between strabismic suppression and binocular rivalry suppression.", "PMID": 955832}
{"id": "pubmed23n0032_1826", "title": "Rabbit corneal epithelial cells grown in vitro without serum.", "content": "Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.", "contents": "Rabbit corneal epithelial cells grown in vitro without serum. Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.", "PMID": 955833}
{"id": "pubmed23n0032_1827", "title": "The measurement of ocular transmittance and irradiation distribution in argon laser irradiated rabbit eyes.", "content": "The transmittance of the ocular media has been measured in vivo in rabbit eyes using an argon laser light source and microthermocouple sensor. The intensity profiles at the cornea and retina were measured with the thermocouple and the ratio of the power associated with the intensity profiles indicated the fraction of light power reaching the retina. Measurements from 10 rabbits indicated a transmittance coefficient of 0.85 for wavelengths between 488 and 514.5 nm.", "contents": "The measurement of ocular transmittance and irradiation distribution in argon laser irradiated rabbit eyes. The transmittance of the ocular media has been measured in vivo in rabbit eyes using an argon laser light source and microthermocouple sensor. The intensity profiles at the cornea and retina were measured with the thermocouple and the ratio of the power associated with the intensity profiles indicated the fraction of light power reaching the retina. Measurements from 10 rabbits indicated a transmittance coefficient of 0.85 for wavelengths between 488 and 514.5 nm.", "PMID": 955834}
{"id": "pubmed23n0032_1828", "title": "An explanation for the long duration of mydriatic effect of atropine in eye.", "content": "The mydriatic effect of topically applied 3H-atropine (2%) was compared in the pigmented rabbits (black fur and dark brown irides) and nonpigmented (albino) atropinesterase-negative rabbits. The duration, t1/2, of the mydriatic effect in the nonpigmented and pigmented rabbit was 43.5 and greater than 96 hours, respectively. At hour 96, the tissue 3H-atropine in the pigmented iris was greater than that in the nonpigmented iris by the factor of eight. The longer duration of mydriatic effect in the pigmented iris is explained by the slow release of the bound drug from the pigment onto the muscarinic receptors.", "contents": "An explanation for the long duration of mydriatic effect of atropine in eye. The mydriatic effect of topically applied 3H-atropine (2%) was compared in the pigmented rabbits (black fur and dark brown irides) and nonpigmented (albino) atropinesterase-negative rabbits. The duration, t1/2, of the mydriatic effect in the nonpigmented and pigmented rabbit was 43.5 and greater than 96 hours, respectively. At hour 96, the tissue 3H-atropine in the pigmented iris was greater than that in the nonpigmented iris by the factor of eight. The longer duration of mydriatic effect in the pigmented iris is explained by the slow release of the bound drug from the pigment onto the muscarinic receptors.", "PMID": 955835}
{"id": "pubmed23n0032_1829", "title": "Origin of ghost cell in Coats' disease.", "content": "A morphological study of Coats' disease was performed with light and electron microscopy. The choroid was intact throughout. The pigment epithelial cells showed proliferative changes, being thickened in two or three rows. The transitional form of ghost cell was observed in contact with the pigment epithelial layer, then migrated toward the subretinal space, and finally invaded the detached retina. It was concluded that the origin of ghost cell in Coats' disease was the pigment epithelium.", "contents": "Origin of ghost cell in Coats' disease. A morphological study of Coats' disease was performed with light and electron microscopy. The choroid was intact throughout. The pigment epithelial cells showed proliferative changes, being thickened in two or three rows. The transitional form of ghost cell was observed in contact with the pigment epithelial layer, then migrated toward the subretinal space, and finally invaded the detached retina. It was concluded that the origin of ghost cell in Coats' disease was the pigment epithelium.", "PMID": 955836}
{"id": "pubmed23n0032_1830", "title": "An analysis of the value of additional diagnostic procedures.", "content": "When several diagnostic procedures are performed on a patient, the probability of his having or not having a specific ailment will change as the result of each procedure is known. This paper describes how such a change is readily calculated using Bayes' theorem. Information theory is also used to find the increment in information from each successive test. Numerical estimates are made using data pertinent to lung X-ray examinations. Finally, some implications for diagnostic examinations in general are drawn.", "contents": "An analysis of the value of additional diagnostic procedures. When several diagnostic procedures are performed on a patient, the probability of his having or not having a specific ailment will change as the result of each procedure is known. This paper describes how such a change is readily calculated using Bayes' theorem. Information theory is also used to find the increment in information from each successive test. Numerical estimates are made using data pertinent to lung X-ray examinations. Finally, some implications for diagnostic examinations in general are drawn.", "PMID": 955837}
{"id": "pubmed23n0032_1831", "title": "Cardiac volume measurement errors.", "content": "Measurements of cardiac volumes based on biplane radiographic data exhibit a cyclical variation as the heart is rotated with respect to the radiologic instrumentation. This study develops a theoretical basis for evaluating the measurement errors due to orientation, that explains the cyclical variation. Since this type of measurement error is always one of overestimation, better accuracy will be obtained if several views are performed at varying orientations and the smallest of the resulting values is used as the measurement.", "contents": "Cardiac volume measurement errors. Measurements of cardiac volumes based on biplane radiographic data exhibit a cyclical variation as the heart is rotated with respect to the radiologic instrumentation. This study develops a theoretical basis for evaluating the measurement errors due to orientation, that explains the cyclical variation. Since this type of measurement error is always one of overestimation, better accuracy will be obtained if several views are performed at varying orientations and the smallest of the resulting values is used as the measurement.", "PMID": 955838}
{"id": "pubmed23n0032_1832", "title": "Organ culture of rodent prostate. Effects of polyamines and testosterone.", "content": "Prostate organs were maintained in culture for 14 days on a plasma clot to which insulin, testosterone, spermine, and spermidine were added. Explants cultured in the control medium (embryo extract and chicken plasma) showed regressive changes and there was complete necrosis by the 5th day.  When cultured in the presence of testosterone plus the control medium the explants continued to maintain their epithelial height and stromal characteristics after 5 days. By the 7th day regressive changes similar to those seen for explants cultured in the absence of testosterone were observed. With spermine and spermidine in the medium along with insulin and testosterone, the stromal and epithelial integrity of the explants was kept intact for 14 days. When either testosterone or spermine was omitted from this list of additives, survival and maintenance of normal morphology were greatly impaired. Omission of spermidine did not affect results if the other chemicals were added. No attempts were made to maintain cultures beyond 14 days.", "contents": "Organ culture of rodent prostate. Effects of polyamines and testosterone. Prostate organs were maintained in culture for 14 days on a plasma clot to which insulin, testosterone, spermine, and spermidine were added. Explants cultured in the control medium (embryo extract and chicken plasma) showed regressive changes and there was complete necrosis by the 5th day.  When cultured in the presence of testosterone plus the control medium the explants continued to maintain their epithelial height and stromal characteristics after 5 days. By the 7th day regressive changes similar to those seen for explants cultured in the absence of testosterone were observed. With spermine and spermidine in the medium along with insulin and testosterone, the stromal and epithelial integrity of the explants was kept intact for 14 days. When either testosterone or spermine was omitted from this list of additives, survival and maintenance of normal morphology were greatly impaired. Omission of spermidine did not affect results if the other chemicals were added. No attempts were made to maintain cultures beyond 14 days.", "PMID": 955844}
{"id": "pubmed23n0032_1833", "title": "Activation of serum complement by contrast media.", "content": "Evidence is presented for the activation of serum complement by contrast media, in vitro and in vivo. Activation as a function of concentration was measured and the increasing order of effectiveness was found to be metrizamide, iothalamate, diatrizoate, acetrizoate, iodipamide and iopanoate. This order is the same as for protein binding and enzyme inhibition. The activation mechanism for iodipamide, and by inference for the other compounds, does not involve gamma-globulin aggregation. Serial daily injections in normal dogs resulted in substantial declines in serum complement over several days. Guinea pigs which were depleted of serum complement with cobra venom factor were found to be no less sensitive to lethal doses of iodipamide than those with normal complement. Implications of these findings are discussed.", "contents": "Activation of serum complement by contrast media. Evidence is presented for the activation of serum complement by contrast media, in vitro and in vivo. Activation as a function of concentration was measured and the increasing order of effectiveness was found to be metrizamide, iothalamate, diatrizoate, acetrizoate, iodipamide and iopanoate. This order is the same as for protein binding and enzyme inhibition. The activation mechanism for iodipamide, and by inference for the other compounds, does not involve gamma-globulin aggregation. Serial daily injections in normal dogs resulted in substantial declines in serum complement over several days. Guinea pigs which were depleted of serum complement with cobra venom factor were found to be no less sensitive to lethal doses of iodipamide than those with normal complement. Implications of these findings are discussed.", "PMID": 955839}
{"id": "pubmed23n0032_1834", "title": "Effect of prolactin on the metabolism of androgens by the rat ventral prostate gland in vitro.", "content": "When homogenates of rat ventral prostate glands were incubated with varying amounts of prolactin (0.2, 0.8, or 2.0 IU per ml), there were significant alterations in the bioconversion of [3H]testosterone and its principal radiometabolites. A 2.0-IU per ml dose of prolactin significantly reduced the metabolism of labeled testosterone in the prostate gland (P less than or equal to 0.01). Similarly, the amounts of [3H]5alpha-androstane-3beta,-17beta-diol ([3H]androstanediol) formed from [3H]testosterone were significantly lower (P less than or equal to 0.05) in the prolactin-treated groups than they were in the controls. Only the highest dose of prolactin (namely, 2.0 IU per ml) impaired the conversion of [3H]testosterone to [3H]17beta-hydroxy-5alpha-androstan-3-one and to [3H]-dihydrotestosterone in the homogenates of rat ventral prostate glands. These findings suggest that prolactin is capable of exerting intracellular actions as evidenced by its ability to interfere with steroidogenesis in androgen-dependent tissues.", "contents": "Effect of prolactin on the metabolism of androgens by the rat ventral prostate gland in vitro. When homogenates of rat ventral prostate glands were incubated with varying amounts of prolactin (0.2, 0.8, or 2.0 IU per ml), there were significant alterations in the bioconversion of [3H]testosterone and its principal radiometabolites. A 2.0-IU per ml dose of prolactin significantly reduced the metabolism of labeled testosterone in the prostate gland (P less than or equal to 0.01). Similarly, the amounts of [3H]5alpha-androstane-3beta,-17beta-diol ([3H]androstanediol) formed from [3H]testosterone were significantly lower (P less than or equal to 0.05) in the prolactin-treated groups than they were in the controls. Only the highest dose of prolactin (namely, 2.0 IU per ml) impaired the conversion of [3H]testosterone to [3H]17beta-hydroxy-5alpha-androstan-3-one and to [3H]-dihydrotestosterone in the homogenates of rat ventral prostate glands. These findings suggest that prolactin is capable of exerting intracellular actions as evidenced by its ability to interfere with steroidogenesis in androgen-dependent tissues.", "PMID": 955845}
{"id": "pubmed23n0032_1835", "title": "Antibiotics and chemotherapeutics in renal lymph. An experimental study in dogs.", "content": "Renal lymph concentrations of ampicillin, carbenicillin, cephacetrile, cephaloridine, and nitrofurantoin were determined in a total of 28 dogs during constant infusion of the drugs together with [125I]iothalamate and [131I]o-iodohippurate for simultaneous determination of glomerular filtration rate and effective renal plasma flow. The lymph concentrations of the radiolabeled clearance substances, serving as a standard were compared to those of the antimicrobial agents. The lymph concentrations of the four antibiotics were all significantly lower than the respective simultaneous arterial plasma concentrations. The nitrofurantoin lymph concentrations were equal to the simultaneous plasma concentrations. It is concluded that the renal lymph concentrations of antibiotics and chemotherapeutics do not exceed the simultaneous plasma concentrations unless the urine flow is obstructed.", "contents": "Antibiotics and chemotherapeutics in renal lymph. An experimental study in dogs. Renal lymph concentrations of ampicillin, carbenicillin, cephacetrile, cephaloridine, and nitrofurantoin were determined in a total of 28 dogs during constant infusion of the drugs together with [125I]iothalamate and [131I]o-iodohippurate for simultaneous determination of glomerular filtration rate and effective renal plasma flow. The lymph concentrations of the radiolabeled clearance substances, serving as a standard were compared to those of the antimicrobial agents. The lymph concentrations of the four antibiotics were all significantly lower than the respective simultaneous arterial plasma concentrations. The nitrofurantoin lymph concentrations were equal to the simultaneous plasma concentrations. It is concluded that the renal lymph concentrations of antibiotics and chemotherapeutics do not exceed the simultaneous plasma concentrations unless the urine flow is obstructed.", "PMID": 955846}
{"id": "pubmed23n0032_1836", "title": "Influences on the rate of absorption of the cholecystographic contrast medium iosumetic acid in man.", "content": "Groups of 5 fasting male and female patients aged 32-83 years were each given 3 gm of the cholecystographic contrast medium iosumetic acid in micronized form (particle size less than 20 mum); Group I with 150 ml tea (standard administration);  Group II with 500 ml tea; Group III with 150 ml tea plus 10 mg metoclopramide (Paspertin) 5 min p. admin. i.v.; and Group IV with 150 ml tea and 3 gm sodium bicarbonate. Blood samples were taken in the period up to 6 hours after administration in order to determine the iodine concentration according to the method of Juegst and Stauch. In Groups I and II maximum blood levels were reached 2.1 +/- 0.4 or 2.0 +/- 0.4 h p. admin. respectively, in Group III 1.6 +/- 0.2 h p. admin (in comparison to Group I: p less than 0.05) and in Group IV the maximum level was reached after only 0.9 +/- 0.2 h p. admin. The difference between Group IV and the other three groups is statistically significant (p less than 0.01). A half-life of 0.3 +/- 0.1 h was calculated for absorption in Group IV.", "contents": "Influences on the rate of absorption of the cholecystographic contrast medium iosumetic acid in man. Groups of 5 fasting male and female patients aged 32-83 years were each given 3 gm of the cholecystographic contrast medium iosumetic acid in micronized form (particle size less than 20 mum); Group I with 150 ml tea (standard administration);  Group II with 500 ml tea; Group III with 150 ml tea plus 10 mg metoclopramide (Paspertin) 5 min p. admin. i.v.; and Group IV with 150 ml tea and 3 gm sodium bicarbonate. Blood samples were taken in the period up to 6 hours after administration in order to determine the iodine concentration according to the method of Juegst and Stauch. In Groups I and II maximum blood levels were reached 2.1 +/- 0.4 or 2.0 +/- 0.4 h p. admin. respectively, in Group III 1.6 +/- 0.2 h p. admin (in comparison to Group I: p less than 0.05) and in Group IV the maximum level was reached after only 0.9 +/- 0.2 h p. admin. The difference between Group IV and the other three groups is statistically significant (p less than 0.01). A half-life of 0.3 +/- 0.1 h was calculated for absorption in Group IV.", "PMID": 955840}
{"id": "pubmed23n0032_1837", "title": "Innervation of pelvic viscera in the rat. Evoked potentials in nerves to bladder and penis (clitoris).", "content": "In 29 rats, responses evoked by pelvic and hypogastric nerve stimulation were recorded from postganglionic nerves to bladder and penis (clitoris). Responses to pelvic nerve stimulation had nonsynapsing and synapsing components. The nonsynapsing component was relatively large in main nerve to penis and small in lateral nerve to penis and nerves to bladder. Pelvic nerve fiber synapsing on pelvic ganglion neurons to bladder had a large subliminal fringe, while fibers synapsing on neurons supplying penis (clitoris) had a small subliminal fringe. Recruitment was greater in nerves to bladder and lateral nerve to penis (clitoris) compared to main nerve to penis (clitoris), indicating more synapsing fibers in the former nerves. Almost all hypogastric fibers to bladder were direct. A small subliminal fringe was demonstrated for hypogastric fibers synapsing on neurons supplying penis. No subliminal fringe was evident for the bladder. Pelvic and hypogastric nerve interaction on pelvic ganglion neurons could not be demonstrated with either single shock or tetanic trains of conditioning stimuli. With antidromic stimulation, conduction velocities of afferent fibers in pelvic nerve ranged from 0.15 m per sec to 2.9 m per sec. In hypogastric nerve they ranged from 0.35 m per sec to 2.8 m per sec.", "contents": "Innervation of pelvic viscera in the rat. Evoked potentials in nerves to bladder and penis (clitoris). In 29 rats, responses evoked by pelvic and hypogastric nerve stimulation were recorded from postganglionic nerves to bladder and penis (clitoris). Responses to pelvic nerve stimulation had nonsynapsing and synapsing components. The nonsynapsing component was relatively large in main nerve to penis and small in lateral nerve to penis and nerves to bladder. Pelvic nerve fiber synapsing on pelvic ganglion neurons to bladder had a large subliminal fringe, while fibers synapsing on neurons supplying penis (clitoris) had a small subliminal fringe. Recruitment was greater in nerves to bladder and lateral nerve to penis (clitoris) compared to main nerve to penis (clitoris), indicating more synapsing fibers in the former nerves. Almost all hypogastric fibers to bladder were direct. A small subliminal fringe was demonstrated for hypogastric fibers synapsing on neurons supplying penis. No subliminal fringe was evident for the bladder. Pelvic and hypogastric nerve interaction on pelvic ganglion neurons could not be demonstrated with either single shock or tetanic trains of conditioning stimuli. With antidromic stimulation, conduction velocities of afferent fibers in pelvic nerve ranged from 0.15 m per sec to 2.9 m per sec. In hypogastric nerve they ranged from 0.35 m per sec to 2.8 m per sec.", "PMID": 955847}
{"id": "pubmed23n0032_1838", "title": "Ureteric vascular and muscle coat innervation in the rat. A quantitative ultrastructural study.", "content": "The application of a quantitative ultrastructural approach to the study of the innervation of the smooth muscle in the wall of the ureter of the rat has revealed major differences in the structure of the nerves in the muscle coat and in the walls of the arterioles. The high density of adrenergic terminals in the periarteriolar nerves is consistent with the distribution of many of the postganglionic sympathetic axons that run in the ureteric nerves to blood vessels and the low density of both adrenergic and cholinergic terminals in the intramuscular nerves implies that the autonomic nervous system plays only a secondary role in controlling the activity of the muscle. The mean size of the intramuscular nerves corresponds to that of the nerves in the submucosa and terminals of the kind that occur in the submucosal nerves are numerous. The function of axons with such terminals and their possible involvement in the transmission to the central nervous system of pain impulses is discussed.", "contents": "Ureteric vascular and muscle coat innervation in the rat. A quantitative ultrastructural study. The application of a quantitative ultrastructural approach to the study of the innervation of the smooth muscle in the wall of the ureter of the rat has revealed major differences in the structure of the nerves in the muscle coat and in the walls of the arterioles. The high density of adrenergic terminals in the periarteriolar nerves is consistent with the distribution of many of the postganglionic sympathetic axons that run in the ureteric nerves to blood vessels and the low density of both adrenergic and cholinergic terminals in the intramuscular nerves implies that the autonomic nervous system plays only a secondary role in controlling the activity of the muscle. The mean size of the intramuscular nerves corresponds to that of the nerves in the submucosa and terminals of the kind that occur in the submucosal nerves are numerous. The function of axons with such terminals and their possible involvement in the transmission to the central nervous system of pain impulses is discussed.", "PMID": 955848}
{"id": "pubmed23n0032_1839", "title": "The autoimmune response to male reproductive tissues of rabbits. IV. Induction of autosensitization to central accessory tissue by the intraabdominal release of endogenous secretions.", "content": "A study is described in which tissue- and secretion-specific autosensitization was induced in adult male rabbits through intraabdominal cannulation of one or more of their central accessory glands. Both primary and secondary humoral antibodies were elicited by this procedure, as determined by tanned cell hemagglutination and passive hemolysis, i.e., complement fixation. The secondary antibody responses were elicited by (i) cannulation of animals which had been previously stimulated by cryosurgery or combined cryosurgery and injection of pooled accessory tissue extract, and (ii) isoinjection of previously cannulated animals with accessory tissue extract. Primary antibody responses were less in animals castrated at the time of cannulation compared to those in noncastrated animals; but, paradoxically, subsequent challenges with tissue extract induced higher secondary antibody titers in castrated animals. These observations may be explained in castrates by (i) reduced uptake of antibodies--a result of the sparing effect on circulating antibodies in castrated animals because of accessory tissue atrophy, or (ii) increased production of antibodies through postcastration immune enhancement. In noncastrates secondary antibody depression may be related to (i) increased absorption of antibodies by an accessory gland complex which is much larger than that found in castrates, or (ii) tolerance, due to exposure of the antibody-producing system to excess accessory tissue antigen.", "contents": "The autoimmune response to male reproductive tissues of rabbits. IV. Induction of autosensitization to central accessory tissue by the intraabdominal release of endogenous secretions. A study is described in which tissue- and secretion-specific autosensitization was induced in adult male rabbits through intraabdominal cannulation of one or more of their central accessory glands. Both primary and secondary humoral antibodies were elicited by this procedure, as determined by tanned cell hemagglutination and passive hemolysis, i.e., complement fixation. The secondary antibody responses were elicited by (i) cannulation of animals which had been previously stimulated by cryosurgery or combined cryosurgery and injection of pooled accessory tissue extract, and (ii) isoinjection of previously cannulated animals with accessory tissue extract. Primary antibody responses were less in animals castrated at the time of cannulation compared to those in noncastrated animals; but, paradoxically, subsequent challenges with tissue extract induced higher secondary antibody titers in castrated animals. These observations may be explained in castrates by (i) reduced uptake of antibodies--a result of the sparing effect on circulating antibodies in castrated animals because of accessory tissue atrophy, or (ii) increased production of antibodies through postcastration immune enhancement. In noncastrates secondary antibody depression may be related to (i) increased absorption of antibodies by an accessory gland complex which is much larger than that found in castrates, or (ii) tolerance, due to exposure of the antibody-producing system to excess accessory tissue antigen.", "PMID": 955849}
{"id": "pubmed23n0032_1840", "title": "Prostatic fluid and tissue concentrations of ampicillin after administration of hetacillin ester (BL-P1761).", "content": "Ampicillin concentrations in prostatic fluid and tissue were studied in dogs after constant intravenous infusion of ampicillin (three dogs) and a lipid-soluble methoxymethyl ester of hetacillin, BL-P1761 (six dogs). Prostatic fluid ampicillin concentrations as determined by bioassay were found to be approximately 15 times higher after administration of hetacillin than after a similar amount of ampicillin. The prostatic fluid concentrations after hetacillin ester administration were approximately one-half of the simultaneous plasma concentrations. This hetacillin ester is thus the first penicillin compound shown to be penetrating the blood-prostatic fluid barrier, resulting in clinically relevant prostatic fluid concentrations.", "contents": "Prostatic fluid and tissue concentrations of ampicillin after administration of hetacillin ester (BL-P1761). Ampicillin concentrations in prostatic fluid and tissue were studied in dogs after constant intravenous infusion of ampicillin (three dogs) and a lipid-soluble methoxymethyl ester of hetacillin, BL-P1761 (six dogs). Prostatic fluid ampicillin concentrations as determined by bioassay were found to be approximately 15 times higher after administration of hetacillin than after a similar amount of ampicillin. The prostatic fluid concentrations after hetacillin ester administration were approximately one-half of the simultaneous plasma concentrations. This hetacillin ester is thus the first penicillin compound shown to be penetrating the blood-prostatic fluid barrier, resulting in clinically relevant prostatic fluid concentrations.", "PMID": 955850}
{"id": "pubmed23n0032_1841", "title": "Structure and function of the rabbit bladder altered by chronic obstruction or cystitis.", "content": "Decreased muscle contractility has been observed in association with intercellular collagen deposition after acute urinary obstruction. Chronically induced (2 months) bladder neck obstruction in rabbits produced similar marked changes in the ultrastructure of the bladder wall, with widening of the intercellular spaces and formation of collagen. Resting intravesical pressure was greater in animals with bladder neck obstruction than in the controls, yet response to stimulation was not impaired. Bacterial cystitis, in contrast, produced no consistent change in ultrastructure but the response of intravesical pressure to pelvic nerve stimulation was reduced. It is concluded that intercellular collagen deposition is not responsible per se for impaired conduction of nerve stimuli; perhaps discrete conduction pathways exist in the detrusor and the intercellular electrotonic spread of action potentials is not essential for contraction.", "contents": "Structure and function of the rabbit bladder altered by chronic obstruction or cystitis. Decreased muscle contractility has been observed in association with intercellular collagen deposition after acute urinary obstruction. Chronically induced (2 months) bladder neck obstruction in rabbits produced similar marked changes in the ultrastructure of the bladder wall, with widening of the intercellular spaces and formation of collagen. Resting intravesical pressure was greater in animals with bladder neck obstruction than in the controls, yet response to stimulation was not impaired. Bacterial cystitis, in contrast, produced no consistent change in ultrastructure but the response of intravesical pressure to pelvic nerve stimulation was reduced. It is concluded that intercellular collagen deposition is not responsible per se for impaired conduction of nerve stimuli; perhaps discrete conduction pathways exist in the detrusor and the intercellular electrotonic spread of action potentials is not essential for contraction.", "PMID": 955851}
{"id": "pubmed23n0032_1842", "title": "Effect of testosterone, dihydrotestosterone, estradiol, and prolactin on the weight and citric acid content of the lateral lobe of the rat prostate.", "content": "This study has been designed to investigate possible potentiation of testosterone and dihydrotestosterone by estradiol and prolactin in augmenting weight and citric acid content of the rat lateral prostate. Using these parameters, we hoped to indirectly assess the effect of estradiol and prolactin on 5alpha-reduction as it acts on the conversion of testosterone to dihydrotesterone. In the first part of the study, 87 castrate and 14 control rats were used to assess the effect of testosterone or dihydrotestosterone when supplemented with estradiol; in the second part of the study, 121 castrate-hypophysectomized rats separated into three groups according to age and stage of maturation were used to assess the effect of prolactin in supplementing testosterone and dihydrotestosterone. Estradiol appears to have a greater effect on 5alpha-reduction than does prolactin; however, other pathways of androgen metabolism are altered by these agents. These alternate pathways are discussed.", "contents": "Effect of testosterone, dihydrotestosterone, estradiol, and prolactin on the weight and citric acid content of the lateral lobe of the rat prostate. This study has been designed to investigate possible potentiation of testosterone and dihydrotestosterone by estradiol and prolactin in augmenting weight and citric acid content of the rat lateral prostate. Using these parameters, we hoped to indirectly assess the effect of estradiol and prolactin on 5alpha-reduction as it acts on the conversion of testosterone to dihydrotesterone. In the first part of the study, 87 castrate and 14 control rats were used to assess the effect of testosterone or dihydrotestosterone when supplemented with estradiol; in the second part of the study, 121 castrate-hypophysectomized rats separated into three groups according to age and stage of maturation were used to assess the effect of prolactin in supplementing testosterone and dihydrotestosterone. Estradiol appears to have a greater effect on 5alpha-reduction than does prolactin; however, other pathways of androgen metabolism are altered by these agents. These alternate pathways are discussed.", "PMID": 955852}
{"id": "pubmed23n0032_1843", "title": "In vitro dog detrusor strip contractile responses and calcium ion activity.", "content": "In vitro detrusor strip experiments show that the presence of calcium ion is essential for spontaneous and bethanechol-induced detrusor contractile activity. 45Ca uptake experiments show that detrusor strips subjected to bethanechol show increased 45Ca uptake with significant decrease in total muscle strip calcium. There is a significant increase in the percentage of calcium exchange, suggesting that calcium efflux has exceeded influx. Kinetic analysis of 45Ca uptake by detrusor strips shows more 45Ca to reside in the slow component of strips following exposure to bethanechol. This suggests that calcium associated with bethanechol-induced contractile activity comes to occupy intracellular or more tightly bound membrane sites.", "contents": "In vitro dog detrusor strip contractile responses and calcium ion activity. In vitro detrusor strip experiments show that the presence of calcium ion is essential for spontaneous and bethanechol-induced detrusor contractile activity. 45Ca uptake experiments show that detrusor strips subjected to bethanechol show increased 45Ca uptake with significant decrease in total muscle strip calcium. There is a significant increase in the percentage of calcium exchange, suggesting that calcium efflux has exceeded influx. Kinetic analysis of 45Ca uptake by detrusor strips shows more 45Ca to reside in the slow component of strips following exposure to bethanechol. This suggests that calcium associated with bethanechol-induced contractile activity comes to occupy intracellular or more tightly bound membrane sites.", "PMID": 955853}
{"id": "pubmed23n0032_1844", "title": "Cathechol-O-methyl transferase activity in the individual human platelet populations.", "content": "COMT activity is present in many animal tissues, but there is no evidence on the presence or the functional role of COMT activity in platelets. The present work reports the results obtained on COMT activity in the individual platelet populations separated by a discontinuous sucrose density gradient. In the young platelet populations the activity is remarkable higher than in the old and light populations. Low values of COMT activity in the old platelets may reflect platelet senescence.", "contents": "Cathechol-O-methyl transferase activity in the individual human platelet populations. COMT activity is present in many animal tissues, but there is no evidence on the presence or the functional role of COMT activity in platelets. The present work reports the results obtained on COMT activity in the individual platelet populations separated by a discontinuous sucrose density gradient. In the young platelet populations the activity is remarkable higher than in the old and light populations. Low values of COMT activity in the old platelets may reflect platelet senescence.", "PMID": 955854}
{"id": "pubmed23n0032_1845", "title": "Urea cycle enzymes in the liver of a dolphin Platanista indi.", "content": "Urea cycle enzymes are all shown to be active in dolphin liver. Acetylglutamate-independent cytoplasmic carbamylphosphate synthase is also present. Arginase is a basic protein, although less markedly basic than the dog enzyme. It is 118 per cent activated by heating at 50 degrees. Optimum pH is 10.5. Co++ and Ni++ inhibit the enzyme. AMP deaminase, glutamicoxaloacetic transaminase, glutamate dehydrogenase and ornithine transaminase are also active in dolphin liver.", "contents": "Urea cycle enzymes in the liver of a dolphin Platanista indi. Urea cycle enzymes are all shown to be active in dolphin liver. Acetylglutamate-independent cytoplasmic carbamylphosphate synthase is also present. Arginase is a basic protein, although less markedly basic than the dog enzyme. It is 118 per cent activated by heating at 50 degrees. Optimum pH is 10.5. Co++ and Ni++ inhibit the enzyme. AMP deaminase, glutamicoxaloacetic transaminase, glutamate dehydrogenase and ornithine transaminase are also active in dolphin liver.", "PMID": 955855}
{"id": "pubmed23n0032_1846", "title": "DNA synthesis in isolated mitochondria: effect of thyroidectomy and of triiodothyronine administration in vivo.", "content": "The incorporation was studied of labelled deoxy-ribonucleosides triphosphates into acid-insoluble material by isolated liver mitochondria, obtained from either normal young male rats, or thyroidectomized at weaning, or thyroidectomized and then treated with triiodothyronine (T3). The incorporation extents in normal animals decreased with the age starting from the weaning until the 210th day. Thyroidectomy reduced the incorporation  extent to about 70% of the values observed in normal animals. During the T3 treatment of thyroidectomized rats a sharp increase of incorporation was observed one day after the first administration, followed by a decrease from the 1st to the 3rd day; successively the incorporation extent remained unchanged until the 12th day. Finally, evidence was provided that T3 doses used were thyreomimetic and definitely lower than the thyreotoxic ones.", "contents": "DNA synthesis in isolated mitochondria: effect of thyroidectomy and of triiodothyronine administration in vivo. The incorporation was studied of labelled deoxy-ribonucleosides triphosphates into acid-insoluble material by isolated liver mitochondria, obtained from either normal young male rats, or thyroidectomized at weaning, or thyroidectomized and then treated with triiodothyronine (T3). The incorporation extents in normal animals decreased with the age starting from the weaning until the 210th day. Thyroidectomy reduced the incorporation  extent to about 70% of the values observed in normal animals. During the T3 treatment of thyroidectomized rats a sharp increase of incorporation was observed one day after the first administration, followed by a decrease from the 1st to the 3rd day; successively the incorporation extent remained unchanged until the 12th day. Finally, evidence was provided that T3 doses used were thyreomimetic and definitely lower than the thyreotoxic ones.", "PMID": 955856}
{"id": "pubmed23n0032_1847", "title": "Changes in the renin-angiotensin system in normotensive and hypertensive women during pregnancy and parturition.", "content": "Plasma renin activity, plasma renin concentration and plasma angiotensin II were measured in 18 primigravid women and 17 multiparous women between 37 and 42 weeks of gestation. Seven of the primigravid and seven of the multiparous women were admitted to hospital because they developed hypertension during pregnancy. In the primigravid group, the mean angiotensin II level was significantly higher in the hypertensive than in the normotensive women. A highly significant correlation was found between diastolic blood pressure and plasma angiotensin II levels. Although a similar trend was seen in the multiparous women, there was a wide scatter of values and the relationship between diastolic blood pressure and plasma angiotensin II was not significant. No correlation could be established between plasma renin concentration and angiotensin II levels. However, there was a trend towards a negative correlation between plasma renin activity and plasma angiotensin II, suggesting that high angiotensin levels may suppress renal renin release. The influence of parturition on plasma angiotensin II levels was investigated in 12 women. Maternal plasma angiotensin II levels were significantly higher in the second stage of labor than in women delivered by elective cesarean section.", "contents": "Changes in the renin-angiotensin system in normotensive and hypertensive women during pregnancy and parturition. Plasma renin activity, plasma renin concentration and plasma angiotensin II were measured in 18 primigravid women and 17 multiparous women between 37 and 42 weeks of gestation. Seven of the primigravid and seven of the multiparous women were admitted to hospital because they developed hypertension during pregnancy. In the primigravid group, the mean angiotensin II level was significantly higher in the hypertensive than in the normotensive women. A highly significant correlation was found between diastolic blood pressure and plasma angiotensin II levels. Although a similar trend was seen in the multiparous women, there was a wide scatter of values and the relationship between diastolic blood pressure and plasma angiotensin II was not significant. No correlation could be established between plasma renin concentration and angiotensin II levels. However, there was a trend towards a negative correlation between plasma renin activity and plasma angiotensin II, suggesting that high angiotensin levels may suppress renal renin release. The influence of parturition on plasma angiotensin II levels was investigated in 12 women. Maternal plasma angiotensin II levels were significantly higher in the second stage of labor than in women delivered by elective cesarean section.", "PMID": 955863}
{"id": "pubmed23n0032_1848", "title": "Is mild preeclampsia (EPH gestosis) harmful to the baby?", "content": "Mild preeclampsia, as defined by a rise in blood pressure to 140/90 mm Hg or more in the third trimester of pregnancy, does not affect the baby either in terms of increased perinatal mortality or a reduction in birth weight. Higher maternal weight gains are associated with greater birth weight of the babies in primigravidae, provided that proteinuria does not occur. Reduction of water retention by diuretic treatment does not lower the incidence of preeclampsia, does reduce the weight of the baby.", "contents": "Is mild preeclampsia (EPH gestosis) harmful to the baby? Mild preeclampsia, as defined by a rise in blood pressure to 140/90 mm Hg or more in the third trimester of pregnancy, does not affect the baby either in terms of increased perinatal mortality or a reduction in birth weight. Higher maternal weight gains are associated with greater birth weight of the babies in primigravidae, provided that proteinuria does not occur. Reduction of water retention by diuretic treatment does not lower the incidence of preeclampsia, does reduce the weight of the baby.", "PMID": 955864}
{"id": "pubmed23n0032_1849", "title": "Changes preceding the development of preeclamptic toxemia.", "content": "No differences were found at the 30th week of pregnancy in total body water, serum sodium, potassium, chloride and osmolality, plasma volume, total protein concentration, intravascular protein mass, serum albumin concentration, intravascular albumin mass, and urinary estriol and pregnanediol in 94 primigravidae who remained normotensive, 35 who developed mild preeclampsia, and 23 who developed severe preeclampsia (i.e. hypertension and significant proteinuria in the third trimester). In twin pregnancies no differences were found between 13 primigravidae who remained normotensive and nine who subsequently developed proteinuria and hypertension.", "contents": "Changes preceding the development of preeclamptic toxemia. No differences were found at the 30th week of pregnancy in total body water, serum sodium, potassium, chloride and osmolality, plasma volume, total protein concentration, intravascular protein mass, serum albumin concentration, intravascular albumin mass, and urinary estriol and pregnanediol in 94 primigravidae who remained normotensive, 35 who developed mild preeclampsia, and 23 who developed severe preeclampsia (i.e. hypertension and significant proteinuria in the third trimester). In twin pregnancies no differences were found between 13 primigravidae who remained normotensive and nine who subsequently developed proteinuria and hypertension.", "PMID": 955865}
{"id": "pubmed23n0032_1850", "title": "Toxemia of pregnancy in Jerusalem. II. The role of diet.", "content": "A dietary history was obtained from 180 patients with preeclampsia and eclampsia, representing 92% of all cases occurring in the pregnant population of West Jerusalem over a defined period. The intake of nutrients and 78 items of diet was compared for each patient and two healthy control subjects who were matched for country of origin, parity, month of delivery, age, year of immigration and years of schooling. All diagnoses were made by the same research team and dietary histories were elicited by one of two experienced dieticians. Comparisons between the 180 patients and their respective control pairs showed a significantly reduced intake of calories, proteins and fats and an increase in consumption of sugar and sweets by the patients. The food items involved, including cheese, eggs, olives and nuts, did not appear consistently, however, in population subgroups defined by age, period of immigration and country of origin. Sixty-six patients reported not having changed their diets during pregnancy and these showed no significant differences in consumption of any of the foods or nutrients at the 1% level, when compared with their matched control subjects. All differences observed were confined to the 114 patients who, for one reason or another, had changed their diets. It is concluded that the differences in diet between patients and control subjects are the result, and not the cause, of the toxemia of pregnancy. Supporting and contradictory evidence in the literature is analyzed, and possible sources of the discrepancies are pointed out.", "contents": "Toxemia of pregnancy in Jerusalem. II. The role of diet. A dietary history was obtained from 180 patients with preeclampsia and eclampsia, representing 92% of all cases occurring in the pregnant population of West Jerusalem over a defined period. The intake of nutrients and 78 items of diet was compared for each patient and two healthy control subjects who were matched for country of origin, parity, month of delivery, age, year of immigration and years of schooling. All diagnoses were made by the same research team and dietary histories were elicited by one of two experienced dieticians. Comparisons between the 180 patients and their respective control pairs showed a significantly reduced intake of calories, proteins and fats and an increase in consumption of sugar and sweets by the patients. The food items involved, including cheese, eggs, olives and nuts, did not appear consistently, however, in population subgroups defined by age, period of immigration and country of origin. Sixty-six patients reported not having changed their diets during pregnancy and these showed no significant differences in consumption of any of the foods or nutrients at the 1% level, when compared with their matched control subjects. All differences observed were confined to the 114 patients who, for one reason or another, had changed their diets. It is concluded that the differences in diet between patients and control subjects are the result, and not the cause, of the toxemia of pregnancy. Supporting and contradictory evidence in the literature is analyzed, and possible sources of the discrepancies are pointed out.", "PMID": 955866}
{"id": "pubmed23n0032_1851", "title": "Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver.", "content": "Valyl-tRNA synthetase from rat liver catalyzes the aminoacylation of tRNA in the presence of a) Mg2+, b) monovalent cations and c) spermine. The degree of aminoacylation with Na+ is equal to that with Mg2+ but the ATP required with Na+ is 25 times lower than that with Mg2+. The aminoacylation becomes almost undetectable in the presence of Na+ when tRNA deprived of Mg2+ is used.", "contents": "Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver. Valyl-tRNA synthetase from rat liver catalyzes the aminoacylation of tRNA in the presence of a) Mg2+, b) monovalent cations and c) spermine. The degree of aminoacylation with Na+ is equal to that with Mg2+ but the ATP required with Na+ is 25 times lower than that with Mg2+. The aminoacylation becomes almost undetectable in the presence of Na+ when tRNA deprived of Mg2+ is used.", "PMID": 955857}
{"id": "pubmed23n0032_1852", "title": "Metabolic aspects of a hypoxia test for placental sufficiency.", "content": "A hypoxia test for placental sufficiency was performed on 24 healthy pregnant women nearing term and 23 high-risk pregnant women in advanced stages of gestation. Hypoxia was induced by inhalation of a breathing mixture containing 7% oxygen through a semi-open system which permitted partial rebreathing, thus preventing hypocapnia. The changes in fetal heart rate (FHR) resulting from maternal hypoxia were recorded continuously by external or internal monitoring. The changes observed in FHR were more marked in high-risk cases. Maternal blood gases were tested before and after 10 min of hypoxia in 10 normal and 10 high-risk patients. In no case was maternal acidosis observed. All pregnant patients regarded as high-risk because of hypertensive disorders or chronic renal disease manifested metabolic alkalosis. This finding cannot be explained. It is regarded as being responsible for the more severe FHR changes through a negative Bohr effect, which reduces the dynamic exchange of oxygen in the placenta. Metabolic alkalosis does not appear in high-risk pregnant women exposed to milder hypoxia by breathing 12% oxygen. The changes in FHR observed during severe maternal hypoxia, i.e., 7% oxygen, are probably due more to maternal metabolic alkalosis than to placental insufficiency. Consequently, the \"severe hypoxia test\" cannot be used as a test for placental insufficiency.", "contents": "Metabolic aspects of a hypoxia test for placental sufficiency. A hypoxia test for placental sufficiency was performed on 24 healthy pregnant women nearing term and 23 high-risk pregnant women in advanced stages of gestation. Hypoxia was induced by inhalation of a breathing mixture containing 7% oxygen through a semi-open system which permitted partial rebreathing, thus preventing hypocapnia. The changes in fetal heart rate (FHR) resulting from maternal hypoxia were recorded continuously by external or internal monitoring. The changes observed in FHR were more marked in high-risk cases. Maternal blood gases were tested before and after 10 min of hypoxia in 10 normal and 10 high-risk patients. In no case was maternal acidosis observed. All pregnant patients regarded as high-risk because of hypertensive disorders or chronic renal disease manifested metabolic alkalosis. This finding cannot be explained. It is regarded as being responsible for the more severe FHR changes through a negative Bohr effect, which reduces the dynamic exchange of oxygen in the placenta. Metabolic alkalosis does not appear in high-risk pregnant women exposed to milder hypoxia by breathing 12% oxygen. The changes in FHR observed during severe maternal hypoxia, i.e., 7% oxygen, are probably due more to maternal metabolic alkalosis than to placental insufficiency. Consequently, the \"severe hypoxia test\" cannot be used as a test for placental insufficiency.", "PMID": 955867}
{"id": "pubmed23n0032_1853", "title": "Preeclampsia and fetal well-being.", "content": "Thirty-three women with preeclamptic toxemia were retrospectively divided into three groups, according to clinical data, urinary estriol excretion, fetal growth, fetal movements and fetal heart recordings. In group 1 (six patients) there was no fetal growth retardation, and fetal motor function and heart rate were normal. In group 2 (17 patients) there was fetal growth retardation, estriol values were usually low, and fetal movements and heart rate were normal. In group 3 (10 patients) fetal movements decreased markedly almost until complete cessation for 12 to 24 h, and pathological changes were present in fetal heart recordings. A classification of preeclamptic toxemia according to these criteria is suggested. Patients who manifest placental metabolic failure, such as the women in group 2, should be hospitalized for observation only, as no immediate danger to the fetus is apparent. If there are signs of severe fetal distress and cardiomotor failure, such as in group 3, prompt delivery is essential to prevent fetal death in utero.", "contents": "Preeclampsia and fetal well-being. Thirty-three women with preeclamptic toxemia were retrospectively divided into three groups, according to clinical data, urinary estriol excretion, fetal growth, fetal movements and fetal heart recordings. In group 1 (six patients) there was no fetal growth retardation, and fetal motor function and heart rate were normal. In group 2 (17 patients) there was fetal growth retardation, estriol values were usually low, and fetal movements and heart rate were normal. In group 3 (10 patients) fetal movements decreased markedly almost until complete cessation for 12 to 24 h, and pathological changes were present in fetal heart recordings. A classification of preeclamptic toxemia according to these criteria is suggested. Patients who manifest placental metabolic failure, such as the women in group 2, should be hospitalized for observation only, as no immediate danger to the fetus is apparent. If there are signs of severe fetal distress and cardiomotor failure, such as in group 3, prompt delivery is essential to prevent fetal death in utero.", "PMID": 955868}
{"id": "pubmed23n0032_1854", "title": "Prognostic parameters in myeloid metaplasia: agnogenic versus postpolycythemic.", "content": "Thirty-nine patients with myelofibrosis and myeloid metaplasia were diagnosed and followed during the period 1955-74. Twenty-eight suffered from agnogenic myeloid metaplasia and 11 from myeloid metaplasia following polycythemia vera. In an attempt to compare the two groups and to define prognostic guidelines, an analysis of survival with respect to various clinical parameters was made. The median survival time of patients with agnogenic myeloid metaplasia was seven years as opposed to only 28 months for the poltpolycythemic group. Older age at diagnosis, a large spleen, or its rapid enlargement, anemia and leukocytosis, but not thrombocytopenia, had a marked negative effect on survival. The clinical condition of most patients was remarkably stable during the entire course of their disease. If a significant drop in hemoglobin occurred, it was sudden, and caused by gastrointestinal hemorrhage or the development of acute leukemia. No demonstrable effect of therapy on survival was found.", "contents": "Prognostic parameters in myeloid metaplasia: agnogenic versus postpolycythemic. Thirty-nine patients with myelofibrosis and myeloid metaplasia were diagnosed and followed during the period 1955-74. Twenty-eight suffered from agnogenic myeloid metaplasia and 11 from myeloid metaplasia following polycythemia vera. In an attempt to compare the two groups and to define prognostic guidelines, an analysis of survival with respect to various clinical parameters was made. The median survival time of patients with agnogenic myeloid metaplasia was seven years as opposed to only 28 months for the poltpolycythemic group. Older age at diagnosis, a large spleen, or its rapid enlargement, anemia and leukocytosis, but not thrombocytopenia, had a marked negative effect on survival. The clinical condition of most patients was remarkably stable during the entire course of their disease. If a significant drop in hemoglobin occurred, it was sudden, and caused by gastrointestinal hemorrhage or the development of acute leukemia. No demonstrable effect of therapy on survival was found.", "PMID": 955869}
{"id": "pubmed23n0032_1855", "title": "Sickle cell thalassemia in an Israeli family.", "content": "Sickle cell thalassemia in an Israeli family of Indian extraction is described. The family demonstrates the segregation of the betaS- and beta-thal genes and the interaction phenomenon known to exist in this condition is demonstrated clearly in the propositus.", "contents": "Sickle cell thalassemia in an Israeli family. Sickle cell thalassemia in an Israeli family of Indian extraction is described. The family demonstrates the segregation of the betaS- and beta-thal genes and the interaction phenomenon known to exist in this condition is demonstrated clearly in the propositus.", "PMID": 955870}
{"id": "pubmed23n0032_1856", "title": "Acute acoustic trauma - a clinical investigation and prognosis in 433 symptomatic soldiers.", "content": "An audiological and clinical study was carried out on 433 soldiers suffering from symptomatic acute acoustic trauma (AAT). The severity of AAT was similar following exposure to different types of firearms. The typical subjective complaints were tinnitus and hearing loss and the more severe the objective hearing loss, the more frequent were the typical subjective symptoms. It seems that there are critical sound pressure levels and critical frequencies below which the remnants of infectious processes in the middle ear may have a protective effect on the cochlea in cases of exposure to noise, whereas above these critical levels and frequencies the injurious influence of noise appears to be enhanced. The best known treatment for AAT seems to be removal from further exposure to noise. Further exposure to noise had a significant worsening influence on the average hearing status, while removal from noise significantly improved the audiometric status.", "contents": "Acute acoustic trauma - a clinical investigation and prognosis in 433 symptomatic soldiers. An audiological and clinical study was carried out on 433 soldiers suffering from symptomatic acute acoustic trauma (AAT). The severity of AAT was similar following exposure to different types of firearms. The typical subjective complaints were tinnitus and hearing loss and the more severe the objective hearing loss, the more frequent were the typical subjective symptoms. It seems that there are critical sound pressure levels and critical frequencies below which the remnants of infectious processes in the middle ear may have a protective effect on the cochlea in cases of exposure to noise, whereas above these critical levels and frequencies the injurious influence of noise appears to be enhanced. The best known treatment for AAT seems to be removal from further exposure to noise. Further exposure to noise had a significant worsening influence on the average hearing status, while removal from noise significantly improved the audiometric status.", "PMID": 955871}
{"id": "pubmed23n0032_1857", "title": "Aplasia of toe phalanges and nails.", "content": "An infant born without toenails and without the middle and distal phalanges of the toes was otherwise normal, on clinical and radiological examination. This is the second case of this entity to be described. It is speculated that an environmental insult at the critical period of fetal toe development may be the cause of the anomaly, but a genetic factor cannot be excluded.", "contents": "Aplasia of toe phalanges and nails. An infant born without toenails and without the middle and distal phalanges of the toes was otherwise normal, on clinical and radiological examination. This is the second case of this entity to be described. It is speculated that an environmental insult at the critical period of fetal toe development may be the cause of the anomaly, but a genetic factor cannot be excluded.", "PMID": 955872}
{"id": "pubmed23n0032_1858", "title": "[The polymorphism of collagen. New viewpoints on the structure and function of connective tissue].", "content": "Recent studies have established that connective tissue contains several chemically and genetically distinct collagens. Here we discuss the nature of these proteins, their occurrence in different tissues and their involvement in disease processes. Types I, II and II collagens show some similarities indicating a common origin. Morphological studies using antibodies against the various collagens indicate that the different collagens occur in different anatomical structures. Alterations in the distribution of these collagens are seen in tissues in certain disease states and may cause the loss of normal tissue function.", "contents": "[The polymorphism of collagen. New viewpoints on the structure and function of connective tissue]. Recent studies have established that connective tissue contains several chemically and genetically distinct collagens. Here we discuss the nature of these proteins, their occurrence in different tissues and their involvement in disease processes. Types I, II and II collagens show some similarities indicating a common origin. Morphological studies using antibodies against the various collagens indicate that the different collagens occur in different anatomical structures. Alterations in the distribution of these collagens are seen in tissues in certain disease states and may cause the loss of normal tissue function.", "PMID": 955889}
{"id": "pubmed23n0032_1859", "title": "[Animal experiments on the relationship between urinary excretion of hydroxyproline and serum hydroxyproline fractions].", "content": "Hydroxyproline excreted withh the urine and the free and peptide-bound hydroxyproline in the serum originate from collagen catabolism in the connective tissue. The relation of protein-bound hydroxyproline in the serum to the collagen metabolism is still contradictory. In normal and lathyritic rats the time dependent changes of specific hydroxyproline activity in the urine and serum after intraperitoneal application of C-14-proline were determined. In normal rats the main part of hydroxyproline excreted in urine and of the protein-bound hydroxyproline in serum have corresponding half-life periods of 0,6-0,7 days and co-ordinate with the neutral salt soluble collagen. Also in lathyritic rats with their increased amount of neutral salt soluble collagen due to an increase collagen synthesis and a retarded transition from soluble to insoluble collagen these two parameters of collagen metabolisms are in agreement.", "contents": "[Animal experiments on the relationship between urinary excretion of hydroxyproline and serum hydroxyproline fractions]. Hydroxyproline excreted withh the urine and the free and peptide-bound hydroxyproline in the serum originate from collagen catabolism in the connective tissue. The relation of protein-bound hydroxyproline in the serum to the collagen metabolism is still contradictory. In normal and lathyritic rats the time dependent changes of specific hydroxyproline activity in the urine and serum after intraperitoneal application of C-14-proline were determined. In normal rats the main part of hydroxyproline excreted in urine and of the protein-bound hydroxyproline in serum have corresponding half-life periods of 0,6-0,7 days and co-ordinate with the neutral salt soluble collagen. Also in lathyritic rats with their increased amount of neutral salt soluble collagen due to an increase collagen synthesis and a retarded transition from soluble to insoluble collagen these two parameters of collagen metabolisms are in agreement.", "PMID": 955890}
{"id": "pubmed23n0032_1860", "title": "[Multiple subcutaneous Trichophyton rubrum abscesses. Pathomorphosis of a generalized superficial tinea due to impaired immunological resistance].", "content": "A patient with chronic polyarthritis is reported who was treated with corticosteroids over several years and also received azathioprine. The patient developed extensive tinea pedum and corporis which was suddenly complicated by multiple subcutaneous abscesses caused by the same organism, Trichophyton rubrum. This type of dermatophytosis has only rarely been described. It shares some characteristics with the maladie dermatophytique of Hadida and Schousboe. Chronic granulomatous dermatophytoses such as granuloma trichophyticum Majocchi or tinea granulomatosa nodularis cruris or acute suppurative dermatophytoses of the kerion Celsi type present different clinical and histological pictures and develop under different pathogenetic conditions.", "contents": "[Multiple subcutaneous Trichophyton rubrum abscesses. Pathomorphosis of a generalized superficial tinea due to impaired immunological resistance]. A patient with chronic polyarthritis is reported who was treated with corticosteroids over several years and also received azathioprine. The patient developed extensive tinea pedum and corporis which was suddenly complicated by multiple subcutaneous abscesses caused by the same organism, Trichophyton rubrum. This type of dermatophytosis has only rarely been described. It shares some characteristics with the maladie dermatophytique of Hadida and Schousboe. Chronic granulomatous dermatophytoses such as granuloma trichophyticum Majocchi or tinea granulomatosa nodularis cruris or acute suppurative dermatophytoses of the kerion Celsi type present different clinical and histological pictures and develop under different pathogenetic conditions.", "PMID": 955893}
{"id": "pubmed23n0032_1861", "title": "[The nodular necrotizing dermatitis].", "content": "Preferring to the antecedent sparse literature an observation of \"dermatitis nodularis necrotica\" is described in a 15 year old girl with constipation showing the typical coarse-nodular necrotizing exanthema especially on the extremities. Histologically there was a perivasculitis with prevailing excessive cellular infiltrates (reticulum cells, granulocytes). Immunological tests (immunoelectrophoresis, fluorescence-histology in skin and intestinal biopsy) were negative. On the other hand staphylococcus aureus (as detected in skin scratches and by intracutaneous tests) seemed to play an etiologic role. Thus \"dermatitis nodularis necrotica\", on clinical grounds somewhat similar to papulo-necrotic tuberculids, seems to be a bacteride with secundary alterations of the skin vessels and not a variant of primary vasculitis.", "contents": "[The nodular necrotizing dermatitis]. Preferring to the antecedent sparse literature an observation of \"dermatitis nodularis necrotica\" is described in a 15 year old girl with constipation showing the typical coarse-nodular necrotizing exanthema especially on the extremities. Histologically there was a perivasculitis with prevailing excessive cellular infiltrates (reticulum cells, granulocytes). Immunological tests (immunoelectrophoresis, fluorescence-histology in skin and intestinal biopsy) were negative. On the other hand staphylococcus aureus (as detected in skin scratches and by intracutaneous tests) seemed to play an etiologic role. Thus \"dermatitis nodularis necrotica\", on clinical grounds somewhat similar to papulo-necrotic tuberculids, seems to be a bacteride with secundary alterations of the skin vessels and not a variant of primary vasculitis.", "PMID": 955894}
{"id": "pubmed23n0032_1862", "title": "[Pigmented cysts. Pigmented epidermal cysts and pigmented trichilemmal cyst].", "content": "The clinical and histopathological picture of the pigmented epidermal cyst and the pigmented trichilemmcyst is illustrated by three case-reports. The pigment is melanin, which is located in the epithelium of the cyst-wall, the corneocytes of the lumen and in macrophages in the dermis. The clinical appearance of melanin pigmented cysts is blue due to the Tyndall-phenomenon.", "contents": "[Pigmented cysts. Pigmented epidermal cysts and pigmented trichilemmal cyst]. The clinical and histopathological picture of the pigmented epidermal cyst and the pigmented trichilemmcyst is illustrated by three case-reports. The pigment is melanin, which is located in the epithelium of the cyst-wall, the corneocytes of the lumen and in macrophages in the dermis. The clinical appearance of melanin pigmented cysts is blue due to the Tyndall-phenomenon.", "PMID": 955895}
{"id": "pubmed23n0032_1863", "title": "[Peticulate acropigmentation, a world-wide disease].", "content": "Acropigmentatio reticularis is a peculiar reticulate pigmentation, which consists of small, angular, somewhat depressed, brown macules of uniform color and involves mainly the extenser surfaces of the distal parts of extremities. It occurs sometimes familial and is regarded as an autosomal dominant disease. Acropigmentatio reticularis was published in 1953 by us. Since then, no other reports of Japan appeared until Griffiths recently found the familial occurrence of a reticulate acropigmentation in South Iran and England. It was then confirmed that this acropigmentation is identical with acropigmentatio reticularis and accordingly proves the appearance of the latter outside of Japan.", "contents": "[Peticulate acropigmentation, a world-wide disease]. Acropigmentatio reticularis is a peculiar reticulate pigmentation, which consists of small, angular, somewhat depressed, brown macules of uniform color and involves mainly the extenser surfaces of the distal parts of extremities. It occurs sometimes familial and is regarded as an autosomal dominant disease. Acropigmentatio reticularis was published in 1953 by us. Since then, no other reports of Japan appeared until Griffiths recently found the familial occurrence of a reticulate acropigmentation in South Iran and England. It was then confirmed that this acropigmentation is identical with acropigmentatio reticularis and accordingly proves the appearance of the latter outside of Japan.", "PMID": 955896}
{"id": "pubmed23n0032_1864", "title": "[Clinical picture of pretibial myxedema and new aspects of diagnosis and pathogenesis].", "content": "We have reported on two cases of pretibial myxedema. In one patient, the pretibial myxedema developed symmetrically half a year after the appearance of endocrine exophthalmos. Recently, acropachy developed as well. In the second case, the pretibial myxedema arose unilaterally in an old cicatricial area, six years after the onset of endocrine exophthalmos. In this syndrome, the development of the whole group of symptoms, dermopathy, ophthalmopathy, and osteopathy at the same time is very rare. The clinical picture is seen in thyroid disorders, as a rule occuring in hyperthyroidism, rarely in hypothyroid or euthyroid states. Apparently, the development of the symptoms does not depend on thyroid hormone production. One of our patients showed conspicuous hyperthyroidism, the other, thyroxin deficiency. The thyreohypophyseal feedback mechanism in this syndrome was examined for the first time by means of TRH stimulation tests. In both patients, thyroid auto-antibodies indicative of autoimmune thyroiditis were demonstrated. A high activity of long acting thyroid stimulator was measured in the one patient's serum.", "contents": "[Clinical picture of pretibial myxedema and new aspects of diagnosis and pathogenesis]. We have reported on two cases of pretibial myxedema. In one patient, the pretibial myxedema developed symmetrically half a year after the appearance of endocrine exophthalmos. Recently, acropachy developed as well. In the second case, the pretibial myxedema arose unilaterally in an old cicatricial area, six years after the onset of endocrine exophthalmos. In this syndrome, the development of the whole group of symptoms, dermopathy, ophthalmopathy, and osteopathy at the same time is very rare. The clinical picture is seen in thyroid disorders, as a rule occuring in hyperthyroidism, rarely in hypothyroid or euthyroid states. Apparently, the development of the symptoms does not depend on thyroid hormone production. One of our patients showed conspicuous hyperthyroidism, the other, thyroxin deficiency. The thyreohypophyseal feedback mechanism in this syndrome was examined for the first time by means of TRH stimulation tests. In both patients, thyroid auto-antibodies indicative of autoimmune thyroiditis were demonstrated. A high activity of long acting thyroid stimulator was measured in the one patient's serum.", "PMID": 955898}
{"id": "pubmed23n0032_1865", "title": "Zinc therapy in acrodermatitis enteropathica.", "content": "An infant is described with acrodermatitis enteropathica, who initially presented with severe and intractable watery diarrhea. Diagnosis was established at the age of eleven weeks. Serum-zinc concentrations were extremely low and urinary zinc excretion was diminished. Eleven days after oral zinc supplement (100 mg elemental zinc per day), the skin lesions had healed. The high therapeutic doses of zinc required for healing are suggestive that zinc malabsorption is an important pathogenetical factor of this disease.", "contents": "Zinc therapy in acrodermatitis enteropathica. An infant is described with acrodermatitis enteropathica, who initially presented with severe and intractable watery diarrhea. Diagnosis was established at the age of eleven weeks. Serum-zinc concentrations were extremely low and urinary zinc excretion was diminished. Eleven days after oral zinc supplement (100 mg elemental zinc per day), the skin lesions had healed. The high therapeutic doses of zinc required for healing are suggestive that zinc malabsorption is an important pathogenetical factor of this disease.", "PMID": 955933}
{"id": "pubmed23n0032_1866", "title": "Treatment of acrodermatitis enteropathica with zinc sulphate. Rerport of 3 cases.", "content": "The therapeutic effect of orally administered zinc in 3 patients affected with Acrodermatitis enteropathica is reported. Single daily doses of zinc resulted in rapid improvement of the general condition of these patients and in clinical remission within a week. Serum, urine and hair zinc levels as well as alkaline phosphatase, which were very low before treatment, returned to normal after therapy. This brief report confirms the efficacy of oral zinc in the treatment of Acrodermatitis enteropathica.", "contents": "Treatment of acrodermatitis enteropathica with zinc sulphate. Rerport of 3 cases. The therapeutic effect of orally administered zinc in 3 patients affected with Acrodermatitis enteropathica is reported. Single daily doses of zinc resulted in rapid improvement of the general condition of these patients and in clinical remission within a week. Serum, urine and hair zinc levels as well as alkaline phosphatase, which were very low before treatment, returned to normal after therapy. This brief report confirms the efficacy of oral zinc in the treatment of Acrodermatitis enteropathica.", "PMID": 955934}
{"id": "pubmed23n0032_1867", "title": "[Blood pressure in adolescence. Measurements and their relation to body development in 745 healthy school children in Basel].", "content": "The average blood pressure measured in a representative sample of 8th grade juveniles (14-16 years) was 120/70 mm Hg. Mean systolic pressure was 5 mm Hg higher in boys. The 90th percentile was 135/80 mm Hg for both sexes. Systolic and diastolic pressure rose with increasing body weight. Correlation with height was less obvious. Difference in weight could not account for all the sex difference in blood pressure. Association of sexual maturation with higher blood pressure values was more pronounced in boys.", "contents": "[Blood pressure in adolescence. Measurements and their relation to body development in 745 healthy school children in Basel]. The average blood pressure measured in a representative sample of 8th grade juveniles (14-16 years) was 120/70 mm Hg. Mean systolic pressure was 5 mm Hg higher in boys. The 90th percentile was 135/80 mm Hg for both sexes. Systolic and diastolic pressure rose with increasing body weight. Correlation with height was less obvious. Difference in weight could not account for all the sex difference in blood pressure. Association of sexual maturation with higher blood pressure values was more pronounced in boys.", "PMID": 955935}
{"id": "pubmed23n0032_1868", "title": "[Peak expiratory flow rate among Swiss boys and girls between 4 to 19 1/2 years of age domiciled in the Canton of Geneva in 1972].", "content": "Peak expiratory flow rate (PEFR) was measured by means of a Wright peak flow meter on a sample of 2865 boys and 2712 girls selected at random in the Canton of Geneva, Switzerland (resident population of Swiss children and adolescents aged 4-19 1/2 in 1972). Technique of measurements and statistical analysis are described with an aim at standardization. Expected percentiles of PEFR (50, 25, 10 and 2.5) versus standing height are presented in tabular form for boys and girls separately. Nomograms are given allowing graphical determination of expected PEFR percentiles in either sex for any given standing height.", "contents": "[Peak expiratory flow rate among Swiss boys and girls between 4 to 19 1/2 years of age domiciled in the Canton of Geneva in 1972]. Peak expiratory flow rate (PEFR) was measured by means of a Wright peak flow meter on a sample of 2865 boys and 2712 girls selected at random in the Canton of Geneva, Switzerland (resident population of Swiss children and adolescents aged 4-19 1/2 in 1972). Technique of measurements and statistical analysis are described with an aim at standardization. Expected percentiles of PEFR (50, 25, 10 and 2.5) versus standing height are presented in tabular form for boys and girls separately. Nomograms are given allowing graphical determination of expected PEFR percentiles in either sex for any given standing height.", "PMID": 955936}
{"id": "pubmed23n0032_1869", "title": "[Reflections on the anorectal pressure profile].", "content": "The results of electromanometric studies of the anorectum in 40 children are presented. Owing to a constant flow of rinsing water through the recording tube and owing to a repeated tracing during the same session, there is a more sensitive picture of the anorectal pressure profile than reported hitherto. In addition, the anorectal pressure values are higher. In healthy infants and in most children with constipation and/or encopresis, the anorectal pressure profile can be divided in 2 different parts. These can be differentiated by their amplitude and according to their shape. In a few patients with constipation, there is no difference between the two parts concerning the shape of the anorectal pressure profile, and the values of the anorectal pressures are below normal. But all children with anal sphincter incontinence have a distinctively abnormal anorectal pressure profile, there is not even a numerical difference between the two parts of the anorectal pressure profile, and the anorectal pressures are very low. The relationship between these two parts of the anorectal pressure profile and the muscles of the pelvic floor are discussed and the significance of the mentioned technique evaluated with regard to the diagnosis and treatment of the anorectal disturbances in childhood.", "contents": "[Reflections on the anorectal pressure profile]. The results of electromanometric studies of the anorectum in 40 children are presented. Owing to a constant flow of rinsing water through the recording tube and owing to a repeated tracing during the same session, there is a more sensitive picture of the anorectal pressure profile than reported hitherto. In addition, the anorectal pressure values are higher. In healthy infants and in most children with constipation and/or encopresis, the anorectal pressure profile can be divided in 2 different parts. These can be differentiated by their amplitude and according to their shape. In a few patients with constipation, there is no difference between the two parts concerning the shape of the anorectal pressure profile, and the values of the anorectal pressures are below normal. But all children with anal sphincter incontinence have a distinctively abnormal anorectal pressure profile, there is not even a numerical difference between the two parts of the anorectal pressure profile, and the anorectal pressures are very low. The relationship between these two parts of the anorectal pressure profile and the muscles of the pelvic floor are discussed and the significance of the mentioned technique evaluated with regard to the diagnosis and treatment of the anorectal disturbances in childhood.", "PMID": 955937}
{"id": "pubmed23n0032_1870", "title": "[Coagulation studies from capillary blood in newborn infants].", "content": "Coagulation studies from capillary blood, consisting of the determination of fibrinogen, factors II and V and proactivator-plasminogen, were done in 104 newborns. In healthy newborns fibrinogen and factor V was found near the adult level, while factor II and proactivator-plasminogen showed a severe deficit. None of the coagulation factors was dependent of gestational age. Fibrinogen was significantly lower in the group of children with perinatal asphyxia. In ill newborns there was a significant correlation between fibrinogen and factor II and fibrinogen and factor V as was between factors II and V. No correlation could be demonstrated between proactivator-plasminogen and any of the other coagulation factors.", "contents": "[Coagulation studies from capillary blood in newborn infants]. Coagulation studies from capillary blood, consisting of the determination of fibrinogen, factors II and V and proactivator-plasminogen, were done in 104 newborns. In healthy newborns fibrinogen and factor V was found near the adult level, while factor II and proactivator-plasminogen showed a severe deficit. None of the coagulation factors was dependent of gestational age. Fibrinogen was significantly lower in the group of children with perinatal asphyxia. In ill newborns there was a significant correlation between fibrinogen and factor II and fibrinogen and factor V as was between factors II and V. No correlation could be demonstrated between proactivator-plasminogen and any of the other coagulation factors.", "PMID": 955938}
{"id": "pubmed23n0032_1871", "title": "Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency in Switzerland. Demonstration of a new variant (G-6-PD Aarau) with chronic nonsphaerocytic haemolytic anaemia.", "content": "A new variant of erythrocytic glucose-6-phosphate dehydrogenase has been found in a family of Swiss origin. It is associated with chronic nonsphaerocytic haemolytic anaemia. The enzyme from the erythrocytes of a young boy of this family was partially purified 110-fold and characterized. It revealed reduced catalytic activity, increased thermolability and two maxima of the pH activity curve at pH 7.0 and 8.5. The Km value for glucose-6-phosphate was reduced, that for NADP was normal. The enzyme showed an increased inhibitor constant for NADPH with respect to NADP. Electrophoretic mobility was normal (B+). 2-Desoxyglucose-6-phosphate and galactose-6-phosphate were utilized at normal rates, whereas the analogue deamino-NADP gave an increased utilization rate. The mother of the propositus could be identified as heterozygous for this enzyme deficiency. Chronic haemolysis is possibly due to the increased thermolability of the variant enzyme.", "contents": "Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency in Switzerland. Demonstration of a new variant (G-6-PD Aarau) with chronic nonsphaerocytic haemolytic anaemia. A new variant of erythrocytic glucose-6-phosphate dehydrogenase has been found in a family of Swiss origin. It is associated with chronic nonsphaerocytic haemolytic anaemia. The enzyme from the erythrocytes of a young boy of this family was partially purified 110-fold and characterized. It revealed reduced catalytic activity, increased thermolability and two maxima of the pH activity curve at pH 7.0 and 8.5. The Km value for glucose-6-phosphate was reduced, that for NADP was normal. The enzyme showed an increased inhibitor constant for NADPH with respect to NADP. Electrophoretic mobility was normal (B+). 2-Desoxyglucose-6-phosphate and galactose-6-phosphate were utilized at normal rates, whereas the analogue deamino-NADP gave an increased utilization rate. The mother of the propositus could be identified as heterozygous for this enzyme deficiency. Chronic haemolysis is possibly due to the increased thermolability of the variant enzyme.", "PMID": 955939}
{"id": "pubmed23n0032_1872", "title": "[Intestinal mucosa in rats with iron deficiency anemia].", "content": "Eighteen albino rats at the age of 15 days were separated in 2 groups of 9 and fed cow's milk exclusively. The milk supplied to the control rats (group I) was supplemented with iron and copper. The months later, the rats were scarificed. The animals of group II were severely anaemic. In each rat, a histological examination of gastric (in group II, only in 2 rats) and jejunal mucosa as well as measurements of total thickness, villous height, epithelial cell height and mitotic index were made. Histology was normal in all the rats. Measurements gave similar results in both groups except for the epithelial cell height which was significantly higher in anaemic rats than in controls. The authors take issue with the methodology followed in previous studies concerning iron-deficient children and suggest that the causal relation between iron deficiency and structural changes of intestinal mucosa in children has not yet been proven.", "contents": "[Intestinal mucosa in rats with iron deficiency anemia]. Eighteen albino rats at the age of 15 days were separated in 2 groups of 9 and fed cow's milk exclusively. The milk supplied to the control rats (group I) was supplemented with iron and copper. The months later, the rats were scarificed. The animals of group II were severely anaemic. In each rat, a histological examination of gastric (in group II, only in 2 rats) and jejunal mucosa as well as measurements of total thickness, villous height, epithelial cell height and mitotic index were made. Histology was normal in all the rats. Measurements gave similar results in both groups except for the epithelial cell height which was significantly higher in anaemic rats than in controls. The authors take issue with the methodology followed in previous studies concerning iron-deficient children and suggest that the causal relation between iron deficiency and structural changes of intestinal mucosa in children has not yet been proven.", "PMID": 955940}
{"id": "pubmed23n0032_1873", "title": "Familial iminoglycinuria with normal intestinal absorption of glycine and imino acids in association with profound mental retardation, a possible \"cerebral phenotype\".", "content": "Spasticity and severe psychomotor retardation in a 2-year-old girl, apparently in expression of chronic progressive encephalopathy, was found in association with prolinuria, hydroxyprolinuria and hyperglycinuria. The pattern and levels of these amino acids in plasma proved to be normal, however, as was their intestinal absorption as well as their concentration in cerebrospinal fluid. The proband appears to be homozygous for iminoglycinuria with an apparent inborn defect of the renal tubular transport system specific to these amino acids. An isolated hyperglycinuria, however, was traced in several obligate heterozygotes in this family. The possibility of a more direct causative link between this anomaly and her clinical manifestations is discussed. Where instances of iminoglycinuria occur in association with cerebral pathology of this type, an analogous impairment of a correspondingly specific cerebral amino acid transport system is postulated.", "contents": "Familial iminoglycinuria with normal intestinal absorption of glycine and imino acids in association with profound mental retardation, a possible \"cerebral phenotype\". Spasticity and severe psychomotor retardation in a 2-year-old girl, apparently in expression of chronic progressive encephalopathy, was found in association with prolinuria, hydroxyprolinuria and hyperglycinuria. The pattern and levels of these amino acids in plasma proved to be normal, however, as was their intestinal absorption as well as their concentration in cerebrospinal fluid. The proband appears to be homozygous for iminoglycinuria with an apparent inborn defect of the renal tubular transport system specific to these amino acids. An isolated hyperglycinuria, however, was traced in several obligate heterozygotes in this family. The possibility of a more direct causative link between this anomaly and her clinical manifestations is discussed. Where instances of iminoglycinuria occur in association with cerebral pathology of this type, an analogous impairment of a correspondingly specific cerebral amino acid transport system is postulated.", "PMID": 955941}
{"id": "pubmed23n0032_1874", "title": "[Happy-puppet syndrome].", "content": "Six cases of this syndrome previously reported in Great Britain and in Canada are reviewed in relation to a personal case, a 17-month-old boy. The syndrome's main features are microbrachycephaly and peculiar face, mental deficiency with euphoria and laughter, ataxia and epilepsy consisting in infantile spasms and grand-mal attacks. Though its precise nature and cause still remain unknown, the syndrome seems neither familial nor progressive.", "contents": "[Happy-puppet syndrome]. Six cases of this syndrome previously reported in Great Britain and in Canada are reviewed in relation to a personal case, a 17-month-old boy. The syndrome's main features are microbrachycephaly and peculiar face, mental deficiency with euphoria and laughter, ataxia and epilepsy consisting in infantile spasms and grand-mal attacks. Though its precise nature and cause still remain unknown, the syndrome seems neither familial nor progressive.", "PMID": 955942}
{"id": "pubmed23n0032_1875", "title": "Quantification and identification of particle movement in epidermis after thermal injury.", "content": "Skin samples from a rabbit hind limb were taken from controls and at 5 min, 2 and 6 hr after a mild thermal injury (60 degrees C for 1 min). Large aggregates of intercellular particles, usually ribosomal in appearance, were seen in 6 hr samples and were accompanied by some peripheral aggregates of particles and by granule-coated vesicles. These structures were present in earlier samples to a lesser extent and were absent from control material. Quantitative assessment showed that intercellular particles apparently increased up to 6 hr whereas peripheral aggregation was maximum at 2 hr. Histochemical analysis confirmed that the particles contained ribonucleoprotein. Other larger particles were seen occasionally and contained carbohydrate. Lymph draining the site showed cellular changes, little change in enzyme activities, and no aggregates of particles.", "contents": "Quantification and identification of particle movement in epidermis after thermal injury. Skin samples from a rabbit hind limb were taken from controls and at 5 min, 2 and 6 hr after a mild thermal injury (60 degrees C for 1 min). Large aggregates of intercellular particles, usually ribosomal in appearance, were seen in 6 hr samples and were accompanied by some peripheral aggregates of particles and by granule-coated vesicles. These structures were present in earlier samples to a lesser extent and were absent from control material. Quantitative assessment showed that intercellular particles apparently increased up to 6 hr whereas peripheral aggregation was maximum at 2 hr. Histochemical analysis confirmed that the particles contained ribonucleoprotein. Other larger particles were seen occasionally and contained carbohydrate. Lymph draining the site showed cellular changes, little change in enzyme activities, and no aggregates of particles.", "PMID": 955969}
{"id": "pubmed23n0032_1876", "title": "Further studies on section thickness measurement.", "content": "A rapid, simple, accurate and reproducible technique for measuring section thickness using an instrument known as a surfometer is described. It has shown that microtome settings cannot be relied upon as a means of monitoring the thickness of sections of quenched or wax-embedded tissue. However, there appeared to be a linear relationship between microtome setting and the thickness of dewaxed sections. Microtome settings seem to provide an accurate indication of the thickness of araldite sections.", "contents": "Further studies on section thickness measurement. A rapid, simple, accurate and reproducible technique for measuring section thickness using an instrument known as a surfometer is described. It has shown that microtome settings cannot be relied upon as a means of monitoring the thickness of sections of quenched or wax-embedded tissue. However, there appeared to be a linear relationship between microtome setting and the thickness of dewaxed sections. Microtome settings seem to provide an accurate indication of the thickness of araldite sections.", "PMID": 955970}
{"id": "pubmed23n0032_1877", "title": "The laser in the Lowry technique for microdissection of freeze-dried tissue slices.", "content": "A new technique for tissue microdissection is described. This procedure, using an u.v.-laser micropreparation instrument, overcomes the extremely time-consuming manual preparation. The u.v.-laser micropreparation design allows fast, precise, reproducible and smear-contamination-free tissue microdissection. The preparation of the tissue sample can be programmed by tracing out the area to be sampled with a non-destructive 0.5 mW He-Ne-laser aiming-beam. The tract is stored in a small electronic unit, which then guides the motor-driven stepping stage on the microscope in the actual dissection run with the u.v.-laser. The laser power is adjustable in the range 4 to 40 kW and controlled by a photo diode displayed on an oscilloscope screen. In the tissue slice, prepared according to Lowry, an unlimited number of cells or tissue compartments can be dissected and afterwards weighed. The procedure described offers a broader use of the quantitative microhistochemical techniques of Lowry and of Neuhoff.", "contents": "The laser in the Lowry technique for microdissection of freeze-dried tissue slices. A new technique for tissue microdissection is described. This procedure, using an u.v.-laser micropreparation instrument, overcomes the extremely time-consuming manual preparation. The u.v.-laser micropreparation design allows fast, precise, reproducible and smear-contamination-free tissue microdissection. The preparation of the tissue sample can be programmed by tracing out the area to be sampled with a non-destructive 0.5 mW He-Ne-laser aiming-beam. The tract is stored in a small electronic unit, which then guides the motor-driven stepping stage on the microscope in the actual dissection run with the u.v.-laser. The laser power is adjustable in the range 4 to 40 kW and controlled by a photo diode displayed on an oscilloscope screen. In the tissue slice, prepared according to Lowry, an unlimited number of cells or tissue compartments can be dissected and afterwards weighed. The procedure described offers a broader use of the quantitative microhistochemical techniques of Lowry and of Neuhoff.", "PMID": 955971}
{"id": "pubmed23n0032_1878", "title": "On the histochemistry of neuronal and glial TPPase.", "content": "Thiamine pyrophosphatase (TPPase) activity in the neuronal Golgi apparatus and in glia cells was investigated using different tissue preparation techniques such as fresh frozen and fixed frozen sections, the effect of ATP as an activator and the fine structural localization of the enzyme activity in glia cell processes and the glio-vascular contact point was observed under the electron microscope. TPPase was demonstrated in the glia cell using only a fixed frozen tissue preparation, activated by ATP with the concentration as low as 0.1 mM. On  the contrary, enzyme activity was detected in the Golgi apparatus of the neuron regardless of tissue preparation, fresh or fixed frozen, or by the addition of ATP to the incubation medium. The identity of the glia cell type showing a positive reaction for TPPase is thought to be part astrocyte and part microglia. The fine structural observation of TPPase localization at the glia cell process ending, particularly at the glio-vascular and glio-fibral (medullated fiber) contact point suggests a functional role of this enzyme for transport of metabolites through glia cell processes to neurons and/or the blood capillary, and also may play a role in regulation of co-carboxylase.", "contents": "On the histochemistry of neuronal and glial TPPase. Thiamine pyrophosphatase (TPPase) activity in the neuronal Golgi apparatus and in glia cells was investigated using different tissue preparation techniques such as fresh frozen and fixed frozen sections, the effect of ATP as an activator and the fine structural localization of the enzyme activity in glia cell processes and the glio-vascular contact point was observed under the electron microscope. TPPase was demonstrated in the glia cell using only a fixed frozen tissue preparation, activated by ATP with the concentration as low as 0.1 mM. On  the contrary, enzyme activity was detected in the Golgi apparatus of the neuron regardless of tissue preparation, fresh or fixed frozen, or by the addition of ATP to the incubation medium. The identity of the glia cell type showing a positive reaction for TPPase is thought to be part astrocyte and part microglia. The fine structural observation of TPPase localization at the glia cell process ending, particularly at the glio-vascular and glio-fibral (medullated fiber) contact point suggests a functional role of this enzyme for transport of metabolites through glia cell processes to neurons and/or the blood capillary, and also may play a role in regulation of co-carboxylase.", "PMID": 955972}
{"id": "pubmed23n0032_1879", "title": "Application of histochemistry to a living organ: polyglucose histochemically synthesized in the paraboloid of the chick accessory cone in vivo by phosphorylase and branching glycosyltransferase.", "content": "Histochemical application in a living animal was tested on the paraboloid of the accessory cone of the chick retina. After the anterior part of the eye had been cut with a Graefe's knife under ether anesthesia, the posterior part was filled with the medium for phosphorylase under exposure to light. The specimens were embedded for routine electron microscopy and the paraboloid of the accessory cone was observed by electron microscopy. Polyglucose particles were synthesized from glucose-l-phosphate in the paraboloid by the activities of phosphorylase and branching glycosyl transferase and found to be in the cytoplasmic matrices. These particles were larger in size and better stainable with lead citrate than those found in the paraboloid of the retina incubated in the medium in vitro by the conventional histochemical method. Overproduction of polyglucose particles was not found in the paraboloid of the retina incubated in the medium in vivo. These findings suggest that polyglucose particles synthesized in vivo have a close resemblance to native glycogen particles and that glycogen metabolism is regulated by the living cell. Glycolysis may not be related to the membranous structures. Therefore, application of enzyme histochemical techniques to the living organ can demonstrate more accurate morphological aspects of metabolism in the cell.", "contents": "Application of histochemistry to a living organ: polyglucose histochemically synthesized in the paraboloid of the chick accessory cone in vivo by phosphorylase and branching glycosyltransferase. Histochemical application in a living animal was tested on the paraboloid of the accessory cone of the chick retina. After the anterior part of the eye had been cut with a Graefe's knife under ether anesthesia, the posterior part was filled with the medium for phosphorylase under exposure to light. The specimens were embedded for routine electron microscopy and the paraboloid of the accessory cone was observed by electron microscopy. Polyglucose particles were synthesized from glucose-l-phosphate in the paraboloid by the activities of phosphorylase and branching glycosyl transferase and found to be in the cytoplasmic matrices. These particles were larger in size and better stainable with lead citrate than those found in the paraboloid of the retina incubated in the medium in vitro by the conventional histochemical method. Overproduction of polyglucose particles was not found in the paraboloid of the retina incubated in the medium in vivo. These findings suggest that polyglucose particles synthesized in vivo have a close resemblance to native glycogen particles and that glycogen metabolism is regulated by the living cell. Glycolysis may not be related to the membranous structures. Therefore, application of enzyme histochemical techniques to the living organ can demonstrate more accurate morphological aspects of metabolism in the cell.", "PMID": 955973}
{"id": "pubmed23n0032_1880", "title": "Histochemical demonstration of cholinesterase activity in the cornea of the rat and the effect of various denervations on the corneal nerves.", "content": "Cholinesterase (ChE) activities of the rat cornea were demonstrated histochemically by using both light and electron microscopes. Acetylcholinesterase (AChE) reaction was localized in the axolemma of the nerves in the corneal stroma. The epithelial cell membranes and the intraepithelial nerve endings also showed AChE reaction. Non-specific cholinesterase (NsChE) activity was observed only in the endothelial cell membranes. Cervical sympathectomy, ciliary ganglionectomy and stereotactic coagulation of the ophthalmic division of the trigeminal nerve were performed in order to study the routes of the AChE-containing nerves to the cornea. The disappearance of AChE-containing nerves was observed only after ophthalmic neurotomy. It is suggested that the AChE-containing nerves are distributed to the rat cornea exclusively via the ophthalamic nerve. They seem to be sensory nerves.", "contents": "Histochemical demonstration of cholinesterase activity in the cornea of the rat and the effect of various denervations on the corneal nerves. Cholinesterase (ChE) activities of the rat cornea were demonstrated histochemically by using both light and electron microscopes. Acetylcholinesterase (AChE) reaction was localized in the axolemma of the nerves in the corneal stroma. The epithelial cell membranes and the intraepithelial nerve endings also showed AChE reaction. Non-specific cholinesterase (NsChE) activity was observed only in the endothelial cell membranes. Cervical sympathectomy, ciliary ganglionectomy and stereotactic coagulation of the ophthalmic division of the trigeminal nerve were performed in order to study the routes of the AChE-containing nerves to the cornea. The disappearance of AChE-containing nerves was observed only after ophthalmic neurotomy. It is suggested that the AChE-containing nerves are distributed to the rat cornea exclusively via the ophthalamic nerve. They seem to be sensory nerves.", "PMID": 955974}
{"id": "pubmed23n0032_1881", "title": "The localization of thiamine pyrophosphatase activity in the acinar cells of stimulated and non-stimulated sublingual glands of the rat.", "content": "The distribution of thiamine pyrophosphatase (TPPase) activity in the acinar cells of the rat sublingual gland has been studied at various stages of the secretory cycle following stimulated secretion. The rats were stimulated to secrete by an intraperitoneal injection of isoproterenol and pilocarpine. In non-stimulated glands, TPPase activity is detected mainly in 3--4 cisternae at the inner concave side of the Golgi complex and in some adjacent condensing vacuoles as in other cells. In the acinar cells 1 to 2 h after stimulation, however, reaction product for the same enzyme activity is detected in the cisternae at the outer aspect, as well as the inner aspect, of the Golgi complex and even in the cisternae of the endoplasmic reticulum (ER). About 4 h after stimulation, TPPase activity becomes concentrated in 3--4 cisternae at the inner concave side of the Golgi complex as in the acinar cells under non-stimulated conditions. Morphological observations of the acinar cells 1 to 2 h after the stimulation have indicated that the reorganization of the Golgi complex and ER is a major event which occurs at this stage. It is possible that this cellular event is related to the occurrence of TPPase activity in those sites which normally show negative reaction in non-stimulated state.", "contents": "The localization of thiamine pyrophosphatase activity in the acinar cells of stimulated and non-stimulated sublingual glands of the rat. The distribution of thiamine pyrophosphatase (TPPase) activity in the acinar cells of the rat sublingual gland has been studied at various stages of the secretory cycle following stimulated secretion. The rats were stimulated to secrete by an intraperitoneal injection of isoproterenol and pilocarpine. In non-stimulated glands, TPPase activity is detected mainly in 3--4 cisternae at the inner concave side of the Golgi complex and in some adjacent condensing vacuoles as in other cells. In the acinar cells 1 to 2 h after stimulation, however, reaction product for the same enzyme activity is detected in the cisternae at the outer aspect, as well as the inner aspect, of the Golgi complex and even in the cisternae of the endoplasmic reticulum (ER). About 4 h after stimulation, TPPase activity becomes concentrated in 3--4 cisternae at the inner concave side of the Golgi complex as in the acinar cells under non-stimulated conditions. Morphological observations of the acinar cells 1 to 2 h after the stimulation have indicated that the reorganization of the Golgi complex and ER is a major event which occurs at this stage. It is possible that this cellular event is related to the occurrence of TPPase activity in those sites which normally show negative reaction in non-stimulated state.", "PMID": 955975}
{"id": "pubmed23n0032_1882", "title": "The cytochemical localization of cholinesterase activity in the developing chick heart.", "content": "The electronhistochemical localization of the cholinesterases of developing chick heart muscle cells has been studied with the aid of a substrate which incorporates an enzyme-susceptible thiolester group and a diazonium group into the same molecule. The embryonic chick heart exhibits cholinesterase activity from Hamilton-Hambruger stage 3 through to four days post hatching. Although enzyme activity is not demonstrated in every location at all stages studied, it has been observed on the nuclear envelope, golgi complex, rough and smooth endoplasmic reticulum, mitochondria and myofilaments. A change in the type of activity has been demonstrated, acetylcholinesterase is found during the first fourteen days of development but thereafter, non-specific cholinesterase is seen instead. As nerves have not been found in relation to the working myocardium, further support is given to the concept that an acetylcholine-cholinesterase system of myogenic origin is involved in spontaneous contraction. Consideration of the distribution of enzyme within the myocardial cell, raises the possibility that cholinesterase may be concerned in a regulatory mechanism of protein synthesis, a suggestion made previously in connection with liver cells.", "contents": "The cytochemical localization of cholinesterase activity in the developing chick heart. The electronhistochemical localization of the cholinesterases of developing chick heart muscle cells has been studied with the aid of a substrate which incorporates an enzyme-susceptible thiolester group and a diazonium group into the same molecule. The embryonic chick heart exhibits cholinesterase activity from Hamilton-Hambruger stage 3 through to four days post hatching. Although enzyme activity is not demonstrated in every location at all stages studied, it has been observed on the nuclear envelope, golgi complex, rough and smooth endoplasmic reticulum, mitochondria and myofilaments. A change in the type of activity has been demonstrated, acetylcholinesterase is found during the first fourteen days of development but thereafter, non-specific cholinesterase is seen instead. As nerves have not been found in relation to the working myocardium, further support is given to the concept that an acetylcholine-cholinesterase system of myogenic origin is involved in spontaneous contraction. Consideration of the distribution of enzyme within the myocardial cell, raises the possibility that cholinesterase may be concerned in a regulatory mechanism of protein synthesis, a suggestion made previously in connection with liver cells.", "PMID": 955976}
{"id": "pubmed23n0032_1883", "title": "On the vegetative network of guinea pig thoracic spinal cord.", "content": "A dense AChE-positive network was visualized by light microscopy in the thoracic spinal cord of grown-up guinea pigs of both sexes (bodyweight 250-300 g). This network connects in a horizontal and vertical direction the preganglionic sympathetic nuclei (n. intermediolateralis pars principalis (ILp), n. intermediolateralis pars funicularis (ILf), n intercalatus spinals (IC), n. intercalatus pars paraependymalis (ICpe), (Petras and Cummings, 1972) all along the thoracic spinal cord. In addition to AChE activity, the bundles of fibers of this network also show a strong formaldehyde-induced NA fluorescence. Electron microscopy demonstrated granular vesicles in the cytoplasm of ILp cells. The surface of the ILp and IC neurons is almost entirely covered with synaptic bottons which have clear and granulated synaptic vesicles. The bundles of fibers consists of parallel myelin-free axones and dendrites. On their cource the axones form varicosities. In the varicosities and in the synaptic enlargements there are also clear and granulated (40-100 nm) vesicles. The probable origin of the vegetative network fibers of guinea pig thoracic spinal cord is discussed.", "contents": "On the vegetative network of guinea pig thoracic spinal cord. A dense AChE-positive network was visualized by light microscopy in the thoracic spinal cord of grown-up guinea pigs of both sexes (bodyweight 250-300 g). This network connects in a horizontal and vertical direction the preganglionic sympathetic nuclei (n. intermediolateralis pars principalis (ILp), n. intermediolateralis pars funicularis (ILf), n intercalatus spinals (IC), n. intercalatus pars paraependymalis (ICpe), (Petras and Cummings, 1972) all along the thoracic spinal cord. In addition to AChE activity, the bundles of fibers of this network also show a strong formaldehyde-induced NA fluorescence. Electron microscopy demonstrated granular vesicles in the cytoplasm of ILp cells. The surface of the ILp and IC neurons is almost entirely covered with synaptic bottons which have clear and granulated synaptic vesicles. The bundles of fibers consists of parallel myelin-free axones and dendrites. On their cource the axones form varicosities. In the varicosities and in the synaptic enlargements there are also clear and granulated (40-100 nm) vesicles. The probable origin of the vegetative network fibers of guinea pig thoracic spinal cord is discussed.", "PMID": 955977}
{"id": "pubmed23n0032_1884", "title": "Membrane alteration of trypsin-treated smooth muscle cells and penetration by antibodies to myosin.", "content": "Smooth muscle cells dispersed by low concentration of trypsin (0.125%) and grown in tissue culture will not bind antibodies against smooth muscle myosin added to the culture medium. These cells will attach, flatten and contract normally. When the trypsin concentration is raised to 0.25%, many of the attached cells will not flatten. Such round cells show uptake of the myosin antibody at the periphery and in the cytoplasm, using the indirect immunofluorescent technique. At a trypsin concentration of 1%, viable cells are no longer observed and all cells show uptake of myosin antibody. It is concluded from these experiments that above a crucial trypsin concentration, the membrane becomes altered sufficiently to permit the penetration of antibodies into the cell interior.", "contents": "Membrane alteration of trypsin-treated smooth muscle cells and penetration by antibodies to myosin. Smooth muscle cells dispersed by low concentration of trypsin (0.125%) and grown in tissue culture will not bind antibodies against smooth muscle myosin added to the culture medium. These cells will attach, flatten and contract normally. When the trypsin concentration is raised to 0.25%, many of the attached cells will not flatten. Such round cells show uptake of the myosin antibody at the periphery and in the cytoplasm, using the indirect immunofluorescent technique. At a trypsin concentration of 1%, viable cells are no longer observed and all cells show uptake of myosin antibody. It is concluded from these experiments that above a crucial trypsin concentration, the membrane becomes altered sufficiently to permit the penetration of antibodies into the cell interior.", "PMID": 955978}
{"id": "pubmed23n0032_1885", "title": "The differentiation of proximal and distal tubules in the male rat kidney: the appearance of aldolase isozymes, aminopeptidase and alkaline phosphatase during ontogeny.", "content": "1. The specific activities of aminopeptidase, alkaline phosphatase and aldolase isozymes were measured in homogenates of kidneys taken at different stages of ontogeny. The cellular localization of these enzymes was studied in cryostat tissue sections using substrate linked assays for aminopeptidase and alkaline phosphatase and the mixed aggregation immuno-cytochemical technique for aldolase isozymes; local enzyme concentrations were estimated photometrically. 2. The presence of both aldolase-A and aldolase-B was demonstrated in all metanephrogenic cells (and at still higher concentrations in collecting tubule cells) of the rat fetus 16 days after conception and in the undifferentiated cells of the neogenetic zone of kidney up to 8 days after birth; no aminopeptidase or alkaline phosphatase could be found in these cells. 3. Measurements made on stained tissue sections show that the shift towards aldolase-B, seen in homogenate analyses, is due to a change in the relative amounts of proximal tubules. No evidence was seen for repression in the synthesis of aldolase-A or aldolase-B monomers in the different kidney cells during ontogeny. 4. Two transitions in the mode of nephron differentiation were observed: one was shortly after birth, the other followed weaning. Before the first transition the concentrations of the enzymes increased to different degrees, such that the enzymes reached concentrations comparable with those as in the cells of adult rats by 2 to 4 days post partum. After the second transition proximal tubule size and specific activity of brush border membrane enzymes increased 3 fold. In contrast, the distal tubules did not increase significantly in size, but their aldolase-A concentration increased 3 fold. 5. Evidence based on enzyme quantification and morphometry in kidney sections is presented to demonstrate that the proximal tubule cells show functional adaptation by two independent mechanisms: specific amplification of gene expression and hypertrophy. In contrast, the distal tubule shows functional adaptation only by specific amplification of gene expression.", "contents": "The differentiation of proximal and distal tubules in the male rat kidney: the appearance of aldolase isozymes, aminopeptidase and alkaline phosphatase during ontogeny. 1. The specific activities of aminopeptidase, alkaline phosphatase and aldolase isozymes were measured in homogenates of kidneys taken at different stages of ontogeny. The cellular localization of these enzymes was studied in cryostat tissue sections using substrate linked assays for aminopeptidase and alkaline phosphatase and the mixed aggregation immuno-cytochemical technique for aldolase isozymes; local enzyme concentrations were estimated photometrically. 2. The presence of both aldolase-A and aldolase-B was demonstrated in all metanephrogenic cells (and at still higher concentrations in collecting tubule cells) of the rat fetus 16 days after conception and in the undifferentiated cells of the neogenetic zone of kidney up to 8 days after birth; no aminopeptidase or alkaline phosphatase could be found in these cells. 3. Measurements made on stained tissue sections show that the shift towards aldolase-B, seen in homogenate analyses, is due to a change in the relative amounts of proximal tubules. No evidence was seen for repression in the synthesis of aldolase-A or aldolase-B monomers in the different kidney cells during ontogeny. 4. Two transitions in the mode of nephron differentiation were observed: one was shortly after birth, the other followed weaning. Before the first transition the concentrations of the enzymes increased to different degrees, such that the enzymes reached concentrations comparable with those as in the cells of adult rats by 2 to 4 days post partum. After the second transition proximal tubule size and specific activity of brush border membrane enzymes increased 3 fold. In contrast, the distal tubules did not increase significantly in size, but their aldolase-A concentration increased 3 fold. 5. Evidence based on enzyme quantification and morphometry in kidney sections is presented to demonstrate that the proximal tubule cells show functional adaptation by two independent mechanisms: specific amplification of gene expression and hypertrophy. In contrast, the distal tubule shows functional adaptation only by specific amplification of gene expression.", "PMID": 955979}
{"id": "pubmed23n0032_1886", "title": "Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds.", "content": "The contribution of tissue tryptophyl residues, with both amino and carboxyl groups linked to the peptide bonds, to visible fluorescence was studied following various histochemical methods. Tryptophan residues of chymotrypsinogen and trypsinogen exhibited visible fluorescence after (1) combined formaldehyde-HCl vapour, (2) combined formaldehyde and acetyl chloride vapour, and (3) glyoxylic acid vapour treatment.", "contents": "Reactivity in tissue tryptophyl units in histofluorescence methods using carbonyl compounds. The contribution of tissue tryptophyl residues, with both amino and carboxyl groups linked to the peptide bonds, to visible fluorescence was studied following various histochemical methods. Tryptophan residues of chymotrypsinogen and trypsinogen exhibited visible fluorescence after (1) combined formaldehyde-HCl vapour, (2) combined formaldehyde and acetyl chloride vapour, and (3) glyoxylic acid vapour treatment.", "PMID": 955980}
{"id": "pubmed23n0032_1887", "title": "Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections.", "content": "Sections from human jejunum were stained histochemically for aminopeptidase and alkaline phosphatase and the aldolase isozymes were detected with the mixed aggregation immuno-cytochemical technique. All enzyme concentrations increased from the bottom to the upper part of the crypt. The concentration of aldolase-A per cell was the same in the upper part of the crypt and the villus, whereas the concentration of the other three enzymes was still higher. Therefore, high amounts of aldolase-B, aminopeptidase and alkaline phosphatase are present in cells highly active in absorption in a fashion similar to that found in the proximal tubule cells of kidney. The relatively undifferentiated cells of the crypts contained both aldolase-A and aldolase-B. Alkaline phosphatase gains its full activity later than aminopeptidase. The synthesis of microvillar membrane enzymes comes to an end earlier than that of the cytosol enzymes.", "contents": "Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections. Sections from human jejunum were stained histochemically for aminopeptidase and alkaline phosphatase and the aldolase isozymes were detected with the mixed aggregation immuno-cytochemical technique. All enzyme concentrations increased from the bottom to the upper part of the crypt. The concentration of aldolase-A per cell was the same in the upper part of the crypt and the villus, whereas the concentration of the other three enzymes was still higher. Therefore, high amounts of aldolase-B, aminopeptidase and alkaline phosphatase are present in cells highly active in absorption in a fashion similar to that found in the proximal tubule cells of kidney. The relatively undifferentiated cells of the crypts contained both aldolase-A and aldolase-B. Alkaline phosphatase gains its full activity later than aminopeptidase. The synthesis of microvillar membrane enzymes comes to an end earlier than that of the cytosol enzymes.", "PMID": 955981}
{"id": "pubmed23n0032_1888", "title": "The presence of aromatic L-amino acid decarboxylase in certain intestinal nerve cells.", "content": "The presence of aromatic 1-amino acid decarboxylase (AADC) in nerve cell bodies of the intrinsic plexuses of the guinea-pig small intestine was demonstrated by incubating segments of intestine with 1-dopa in the presence of an inhibitor of monoamine oxidase, pargyline. After such incubation, some nerve cell bodies gave a fluorescence histochemical reaction indicative of the presence of a decarboxylated product of 1-dopa, probably dopamine. No fluorescence reaction occurred in the unincubated control or if the inhibitor of AADC, RO 4-4602, was included in the incubation mixture. The AADC-containing cell bodies apparently do not take up and store dopamine, because no fluorescence could be detected after incubation with dopamine and a monoamine oxidase inhibitor. The AADC-containing cells were found in about half of the ganglia of the submucous plexus of the guinea-pig small intestine, but were considerably less frequent in the myenteric plexus. They were also found in the other areas examined in this study, that is, in both enteric plexuses of the guinea-pig distal colon and of the small intestines of rabbits and rats.", "contents": "The presence of aromatic L-amino acid decarboxylase in certain intestinal nerve cells. The presence of aromatic 1-amino acid decarboxylase (AADC) in nerve cell bodies of the intrinsic plexuses of the guinea-pig small intestine was demonstrated by incubating segments of intestine with 1-dopa in the presence of an inhibitor of monoamine oxidase, pargyline. After such incubation, some nerve cell bodies gave a fluorescence histochemical reaction indicative of the presence of a decarboxylated product of 1-dopa, probably dopamine. No fluorescence reaction occurred in the unincubated control or if the inhibitor of AADC, RO 4-4602, was included in the incubation mixture. The AADC-containing cell bodies apparently do not take up and store dopamine, because no fluorescence could be detected after incubation with dopamine and a monoamine oxidase inhibitor. The AADC-containing cells were found in about half of the ganglia of the submucous plexus of the guinea-pig small intestine, but were considerably less frequent in the myenteric plexus. They were also found in the other areas examined in this study, that is, in both enteric plexuses of the guinea-pig distal colon and of the small intestines of rabbits and rats.", "PMID": 955982}
{"id": "pubmed23n0032_1889", "title": "Lipids in the skin of a cat-fish Heteropneustes fossilis (Bloch) (Heteropneustidae, Pisces). A histochemical investigation.", "content": "Localization and characterization of different lipids in the cellular constituents of the skin of Heteropneustes fossilis has been made using several histochemical techniques. High contents of cholesterol, its esters and phospholipids have been correlated with the metabolically active state of the basal cells undergoing cell proliferation and differentiation. The polygonal cells in the outermost layer of the epidermis, though rich in phospholipid contain small amounts of cholesterol and its esters. This has been correlated with the metabolically less active state of theses cells. Neutral lipids and phospholipids in the polygonal cells of the outermost layer may contribute to the contents of surface slime and act as an efficient barrier for the penetration of water through the skin. The deposits of neutral lipids in the subcutis may provide energy during the period of fasting, act as a barrier for water diffusion through the skin and serve as shock absorbing pads protecting the fish from mechanical injury.", "contents": "Lipids in the skin of a cat-fish Heteropneustes fossilis (Bloch) (Heteropneustidae, Pisces). A histochemical investigation. Localization and characterization of different lipids in the cellular constituents of the skin of Heteropneustes fossilis has been made using several histochemical techniques. High contents of cholesterol, its esters and phospholipids have been correlated with the metabolically active state of the basal cells undergoing cell proliferation and differentiation. The polygonal cells in the outermost layer of the epidermis, though rich in phospholipid contain small amounts of cholesterol and its esters. This has been correlated with the metabolically less active state of theses cells. Neutral lipids and phospholipids in the polygonal cells of the outermost layer may contribute to the contents of surface slime and act as an efficient barrier for the penetration of water through the skin. The deposits of neutral lipids in the subcutis may provide energy during the period of fasting, act as a barrier for water diffusion through the skin and serve as shock absorbing pads protecting the fish from mechanical injury.", "PMID": 955983}
{"id": "pubmed23n0032_1890", "title": "Acetylcholinesterase distribution in chick spinal cord cultures. A light and electron microscope study.", "content": "Explants of 10--12 day chick embryo spinal cord were cultured by coverslip-roller tube method for 3-80 days. The cellular and subcellular localization of acetylcholinesterase activity in cultured neurons was studied by the thiocholine techniques of Karnovsky and Roots and Lewis and Shute. At the light microscopic level, acetylcholinesterase was demonstrated in the neurons of both ventral and dorsal horn regions. Occasionally neurons migrated in the outgrowth zone exhibited strong intracellular activity. At the electron microscopic level, acetylcholinesterase activity was found in the nuclear envelope, granular endoplasmic reticulum and the Golgi apparatus of the neurons. No enzyme reaction was detected in the glial cell cytoplasm.", "contents": "Acetylcholinesterase distribution in chick spinal cord cultures. A light and electron microscope study. Explants of 10--12 day chick embryo spinal cord were cultured by coverslip-roller tube method for 3-80 days. The cellular and subcellular localization of acetylcholinesterase activity in cultured neurons was studied by the thiocholine techniques of Karnovsky and Roots and Lewis and Shute. At the light microscopic level, acetylcholinesterase was demonstrated in the neurons of both ventral and dorsal horn regions. Occasionally neurons migrated in the outgrowth zone exhibited strong intracellular activity. At the electron microscopic level, acetylcholinesterase activity was found in the nuclear envelope, granular endoplasmic reticulum and the Golgi apparatus of the neurons. No enzyme reaction was detected in the glial cell cytoplasm.", "PMID": 955984}
{"id": "pubmed23n0032_1891", "title": "Ultrastructural identification of monoaminergic synaptosomes from one day old rat brain.", "content": "Synaptosomes from one day old and adult rat brain were studied. Specific cytochemical methods for demonstrating monoaminergic (MA) nerve endings were used. Permanganate fixation after preincubation with 5-OHDA or alpha-methyl-NA demonstrated MA synaptosomes. Their number was small in the adult (less than 1 %) and still smaller in the one day old rat brain. The MA synaptosomes from developing rats were different from the adult ones. The large amount of endoplasmic reticulum in developing synaptosomes suggests that granular vesicles are formed from endoplasmic reticulum in nerve endings.", "contents": "Ultrastructural identification of monoaminergic synaptosomes from one day old rat brain. Synaptosomes from one day old and adult rat brain were studied. Specific cytochemical methods for demonstrating monoaminergic (MA) nerve endings were used. Permanganate fixation after preincubation with 5-OHDA or alpha-methyl-NA demonstrated MA synaptosomes. Their number was small in the adult (less than 1 %) and still smaller in the one day old rat brain. The MA synaptosomes from developing rats were different from the adult ones. The large amount of endoplasmic reticulum in developing synaptosomes suggests that granular vesicles are formed from endoplasmic reticulum in nerve endings.", "PMID": 955985}
{"id": "pubmed23n0032_1892", "title": "[The significance of the CNS for the physiology of hearing (author's transl)].", "content": "Some general aspects of auditory perception (funnelling, contrast, pattern recognition, form perception, methodological limitations) are discussed in the first part of this presentation. The modern concept of the hydrodynamics of the inner ear, including electronic modelling of the function of the basilar membrane is their dealth with. For the encoding and decoding processes of the auditory system the different grades of intensity function at the different neuronal levels is compared with that of the envelope of the basilar membranes deflection. The influence of the efferent system is discussed. In a third section of the paper some new results from our department are reported concerning the single unit activity at geniculate level recorded from the awake cat by means of a telemetric system. Neurophysiological correlates to vowel- and consonant detectors and their relations to be the phonem recognition of speech could be demonstrated. Finally, the most recent state of objective audiometry is discussed (DC-potentials, simultaneous records of electrocochleogram and cortically evoked activity, relations to EEG's power spectrum, and brains changes in vigilance) with a view of future approaches to ERA.", "contents": "[The significance of the CNS for the physiology of hearing (author's transl)]. Some general aspects of auditory perception (funnelling, contrast, pattern recognition, form perception, methodological limitations) are discussed in the first part of this presentation. The modern concept of the hydrodynamics of the inner ear, including electronic modelling of the function of the basilar membrane is their dealth with. For the encoding and decoding processes of the auditory system the different grades of intensity function at the different neuronal levels is compared with that of the envelope of the basilar membranes deflection. The influence of the efferent system is discussed. In a third section of the paper some new results from our department are reported concerning the single unit activity at geniculate level recorded from the awake cat by means of a telemetric system. Neurophysiological correlates to vowel- and consonant detectors and their relations to be the phonem recognition of speech could be demonstrated. Finally, the most recent state of objective audiometry is discussed (DC-potentials, simultaneous records of electrocochleogram and cortically evoked activity, relations to EEG's power spectrum, and brains changes in vigilance) with a view of future approaches to ERA.", "PMID": 955986}
{"id": "pubmed23n0032_1893", "title": "[An oesophagotracheal Fistula following prolonged intubation (author's transl)].", "content": "Attention is drawn to the case of an oesophagotracheal fistula following prolonged intubation. A combination of physical factors, the use of unsuitable cuffs and a nasogastric feeding tube, are considered to be the cause. Surgical repair of the fistula is advised as soon as possible. While primary closure of the oesophageal defect is always possible, suture of the membraneous trachea may be impossible and a graft may be necessary to cover the defect. For this is recommended oral mucosa, which achieved very good result in this reported case.", "contents": "[An oesophagotracheal Fistula following prolonged intubation (author's transl)]. Attention is drawn to the case of an oesophagotracheal fistula following prolonged intubation. A combination of physical factors, the use of unsuitable cuffs and a nasogastric feeding tube, are considered to be the cause. Surgical repair of the fistula is advised as soon as possible. While primary closure of the oesophageal defect is always possible, suture of the membraneous trachea may be impossible and a graft may be necessary to cover the defect. For this is recommended oral mucosa, which achieved very good result in this reported case.", "PMID": 955987}
{"id": "pubmed23n0032_1894", "title": "[I V. Vocal disturbances in neuro-psychiatric illnesses and in laryngeal paralyses (author's transl)].", "content": "In the fourth part of a series on applied phoniatrics, a review of vocal disorders as symptoms of neurological and psychiatric diseases is given. Clinical characteristics, diagnosis, as well as laryngologic and phoniatric aspects of therapy of vocal cord paralysis are also described.", "contents": "[I V. Vocal disturbances in neuro-psychiatric illnesses and in laryngeal paralyses (author's transl)]. In the fourth part of a series on applied phoniatrics, a review of vocal disorders as symptoms of neurological and psychiatric diseases is given. Clinical characteristics, diagnosis, as well as laryngologic and phoniatric aspects of therapy of vocal cord paralysis are also described.", "PMID": 955988}
{"id": "pubmed23n0032_1895", "title": "[Prefabricated complex wire replacement of middle ear ossicles (author's transl)].", "content": "This article comments on two prefabricated and linked complexes of wire that were used to replace the malleus and incus, and the malleus, incus and stapes.", "contents": "[Prefabricated complex wire replacement of middle ear ossicles (author's transl)]. This article comments on two prefabricated and linked complexes of wire that were used to replace the malleus and incus, and the malleus, incus and stapes.", "PMID": 955989}
{"id": "pubmed23n0032_1896", "title": "[Narcosis as anesthesia in middle ear surgery (author's transl)].", "content": "Four essential points exist in the use of narcosis for surgery of the middle ear: 1. In addition to local anesthesia, any method of narcosis for anesthesia with endotracheal intubation is suitable for surgery. 2. Surgical procedures utilizing the operating microscope make immobility of the patient mandatory. 3. Establishment of a relatively bloodless surgical field by special techniques (such as use of local vasoconstrictors or controlled hypotension with ganglionic blocking agents or halothane) can be employed. 4. Altered middle ear pressure by nitrous oxide (causing a rise in pressure by 3-4 cm H20) can impede operative procedures.", "contents": "[Narcosis as anesthesia in middle ear surgery (author's transl)]. Four essential points exist in the use of narcosis for surgery of the middle ear: 1. In addition to local anesthesia, any method of narcosis for anesthesia with endotracheal intubation is suitable for surgery. 2. Surgical procedures utilizing the operating microscope make immobility of the patient mandatory. 3. Establishment of a relatively bloodless surgical field by special techniques (such as use of local vasoconstrictors or controlled hypotension with ganglionic blocking agents or halothane) can be employed. 4. Altered middle ear pressure by nitrous oxide (causing a rise in pressure by 3-4 cm H20) can impede operative procedures.", "PMID": 955990}
{"id": "pubmed23n0032_1897", "title": "[Pathology and pathogenesis of chronic secretory otitis media in childhood (author's transl)].", "content": "The pathology and pathogenesis of chronic secretory otitis media is described on the bais of quantitative studies of the mucosa, using whole mounts as well as serial sections of normal and biopsy specimens. The pathogenetic is divided into three stages. The initial stage, i.e. the period from the action of the aetiological factor on the middle ear mucosa till the accumulation of mucous secretion, is characterized by inflammatory processes by way of vascular proliferation and dilatation and lymphocytic infiltration. These processes trigger hyperplasia and differentiation of the epithelial cells with subsequent metaplasia of the epithelium into pseudostratified columnar ciliated epithelium with significantly increased goblet cell density and formation of mucous glands. The secretion is predominantly exudative with an admixture of mucus. The secretory stage is dominated by secretion of mucus from active mucous glands and goblet cells, the number of which has been grossly raised. The secretion accumulating in the middle ear is chiefly mucous. During the degenerative stage mucus production abates, and the middle ear returns to normal provided that irreversible adhesive changes have not occurred. The decline in mucus production is due to degeneration and inactivation of the glands combined with decreasing goblet cell density. The epithelium normalizes into cubical epithelium in one or two layers, or through metaplasia changes into secretorily inactive stratified squamous epithelium. The aetiological role of the auditory tube and the importance of tubal function is discussed. The relationship between the ability of the mucosa to produce mucus and the capability of the tube to carry it away is found to be decisive for the clinical course and the severity of the disease.", "contents": "[Pathology and pathogenesis of chronic secretory otitis media in childhood (author's transl)]. The pathology and pathogenesis of chronic secretory otitis media is described on the bais of quantitative studies of the mucosa, using whole mounts as well as serial sections of normal and biopsy specimens. The pathogenetic is divided into three stages. The initial stage, i.e. the period from the action of the aetiological factor on the middle ear mucosa till the accumulation of mucous secretion, is characterized by inflammatory processes by way of vascular proliferation and dilatation and lymphocytic infiltration. These processes trigger hyperplasia and differentiation of the epithelial cells with subsequent metaplasia of the epithelium into pseudostratified columnar ciliated epithelium with significantly increased goblet cell density and formation of mucous glands. The secretion is predominantly exudative with an admixture of mucus. The secretory stage is dominated by secretion of mucus from active mucous glands and goblet cells, the number of which has been grossly raised. The secretion accumulating in the middle ear is chiefly mucous. During the degenerative stage mucus production abates, and the middle ear returns to normal provided that irreversible adhesive changes have not occurred. The decline in mucus production is due to degeneration and inactivation of the glands combined with decreasing goblet cell density. The epithelium normalizes into cubical epithelium in one or two layers, or through metaplasia changes into secretorily inactive stratified squamous epithelium. The aetiological role of the auditory tube and the importance of tubal function is discussed. The relationship between the ability of the mucosa to produce mucus and the capability of the tube to carry it away is found to be decisive for the clinical course and the severity of the disease.", "PMID": 955991}
{"id": "pubmed23n0032_1898", "title": "[Secondary radiogenic cancer of the larynx (author's transl)].", "content": "Based on a series of 32 cases of late recurrence of early stage vocal cord cancer, the question of the pathogenesis of these lesions is discussed. Late recurrence, 5 to 18 years after treatment of the first tumour was observed in 3 cases after a laryngofissure procedure only and in 29 cases after laryngofissure combined with postoperative irradiation or after pure irradiation therapy. The clinical and statistical data and the histological picture of the second tumour show with a high degree of certainty that these second tumours are caused by the cancerogenic side-effect of the primary irradation, which destroyed the primary tumour. The danger that a patient, cured from his first cancer by irradiation, develops a second radiogenic cancer rises from year to year that the patient survives and certainly is much higher than had been suspected. Irradiation of a vocal cord cancer in a young patient with a relatively high life expectancy should no longer by used. Irradiated patients must be reviewed until the end of their lifes.", "contents": "[Secondary radiogenic cancer of the larynx (author's transl)]. Based on a series of 32 cases of late recurrence of early stage vocal cord cancer, the question of the pathogenesis of these lesions is discussed. Late recurrence, 5 to 18 years after treatment of the first tumour was observed in 3 cases after a laryngofissure procedure only and in 29 cases after laryngofissure combined with postoperative irradiation or after pure irradiation therapy. The clinical and statistical data and the histological picture of the second tumour show with a high degree of certainty that these second tumours are caused by the cancerogenic side-effect of the primary irradation, which destroyed the primary tumour. The danger that a patient, cured from his first cancer by irradiation, develops a second radiogenic cancer rises from year to year that the patient survives and certainly is much higher than had been suspected. Irradiation of a vocal cord cancer in a young patient with a relatively high life expectancy should no longer by used. Irradiated patients must be reviewed until the end of their lifes.", "PMID": 955992}
{"id": "pubmed23n0032_1899", "title": "[Reconstruction of the larynx following laryngectomy (author's transl)].", "content": "A pedicled flap of chest skin is sutured to a tube and interposed between tracheostome and hypopharynx, similar to Asai's method of laryngeal reconstruction. The technique is described, and the dangers and disadvantages are mentioned.", "contents": "[Reconstruction of the larynx following laryngectomy (author's transl)]. A pedicled flap of chest skin is sutured to a tube and interposed between tracheostome and hypopharynx, similar to Asai's method of laryngeal reconstruction. The technique is described, and the dangers and disadvantages are mentioned.", "PMID": 955993}
{"id": "pubmed23n0032_1900", "title": "[Applied phoniatry. V. Laryngeal diagnosis and rehabilitation after laryngeal surgery (author's transl)].", "content": "In the last of 5 articles on the benefits of applied phoniatrics hints are given on the differentiation between laryngeal inflammations and tumours. Voice rehabiliation after laryngeal surgery (laryngofissure, partial laryngectomy, total laryngetomy) is demonstrated. Disorders of voice caused by intubation are also described.", "contents": "[Applied phoniatry. V. Laryngeal diagnosis and rehabilitation after laryngeal surgery (author's transl)]. In the last of 5 articles on the benefits of applied phoniatrics hints are given on the differentiation between laryngeal inflammations and tumours. Voice rehabiliation after laryngeal surgery (laryngofissure, partial laryngectomy, total laryngetomy) is demonstrated. Disorders of voice caused by intubation are also described.", "PMID": 955994}
{"id": "pubmed23n0032_1901", "title": "[Intensive care in ENT medicine, head and neck surgery (author's transl)].", "content": "In intensive medicine we distinguish between intensive care units which have mostly observatory and monitoring functions and intensive therapy units. For the surgical patients of the ear, nose, and throat department the intensive care units are probably of greater significance. The following play an important role: Preoperative work-up including laboratory fluoroscopy and x-rays, pulmonary function tests and blood gas analysis; induction of anesthesia, access to good veins, gastric intubation, urinary bladder catheterization; maintenance of anesthesia and postoperative observation, fluid balance, oral and parenteral feeding, tracheostomy care, bronchial toilet; inhalation therapy. The last receives an extensive discussion.", "contents": "[Intensive care in ENT medicine, head and neck surgery (author's transl)]. In intensive medicine we distinguish between intensive care units which have mostly observatory and monitoring functions and intensive therapy units. For the surgical patients of the ear, nose, and throat department the intensive care units are probably of greater significance. The following play an important role: Preoperative work-up including laboratory fluoroscopy and x-rays, pulmonary function tests and blood gas analysis; induction of anesthesia, access to good veins, gastric intubation, urinary bladder catheterization; maintenance of anesthesia and postoperative observation, fluid balance, oral and parenteral feeding, tracheostomy care, bronchial toilet; inhalation therapy. The last receives an extensive discussion.", "PMID": 955996}
{"id": "pubmed23n0032_1902", "title": "[Round window membrane pathology in sudden deafness (author's transl)].", "content": "Exploration of the middle ear was performed on 15 patients with sudden deafness that had failed to improve after intravenous infusion of Eupaverin and Rheomacrodex. Three patients had abnormalities of the round window membrane, in 2 there was a perilymphatic fistula and in 1 an abnormal bulging of the membrane with no transmission of perilymphatic fluid movement. These 3 patients presented with a history of exertion or stress prior to the onset of symptoms. Surgical closure ofthe 2 fistulae led to improvement of hearing, and perilymphatic decompression in the third patient improved the tinnitus but not the deafness. The possible causes of round window pathology are discussed.", "contents": "[Round window membrane pathology in sudden deafness (author's transl)]. Exploration of the middle ear was performed on 15 patients with sudden deafness that had failed to improve after intravenous infusion of Eupaverin and Rheomacrodex. Three patients had abnormalities of the round window membrane, in 2 there was a perilymphatic fistula and in 1 an abnormal bulging of the membrane with no transmission of perilymphatic fluid movement. These 3 patients presented with a history of exertion or stress prior to the onset of symptoms. Surgical closure ofthe 2 fistulae led to improvement of hearing, and perilymphatic decompression in the third patient improved the tinnitus but not the deafness. The possible causes of round window pathology are discussed.", "PMID": 955997}
{"id": "pubmed23n0032_1903", "title": "[Epidural haematoma (Hygroma) with a mastoid extension (author's transl)].", "content": "A traumatic epidural haematoma, which extended both infra- and supra-tentorially is reported. It also extended into the mastoid antrum and air cell system and was diagnosed by surgical exploration. The radiological findings and treatment are discussed.", "contents": "[Epidural haematoma (Hygroma) with a mastoid extension (author's transl)]. A traumatic epidural haematoma, which extended both infra- and supra-tentorially is reported. It also extended into the mastoid antrum and air cell system and was diagnosed by surgical exploration. The radiological findings and treatment are discussed.", "PMID": 955998}
{"id": "pubmed23n0032_1904", "title": "[Experiences with polyethylene ventilation tubes in children with recurrent middle ear inflammation (author's transl)].", "content": "Middle-ear ventilation tubes (of the Sheehy modification) were placed in 401 ears of 209 children under 16 years of age as treatment for recurrent middle-ear infection. The same procedure was later required in 26% of the cases studied because of further recurrence of middle-ear infection. In four per cent of all patients studied, the ventilating tube failed to cure middle ear disease in spite of controlled concomitant antibacterial therapy. In these cases, an early extrusion of the tube was commonly found. Among all patients, the tube was spontaneously extruded in 76 per cent of the ears, whereas the intubation time was longer than half a year in 53 per cent of the cases. No complications from insertion of the ventilation tubes was seen in the present series of patients. The tympanic membrane was found to be normal in 71 per cent of all cases when follow-up examination was obtained three months after completion in intubation. However, a permanent tympanic membrane perforation was found in five ears (1% of patients). The present study has confirmed the usefulness of middle-ear ventilation tubes, particularly in patients with recurrent middle-ear infections.", "contents": "[Experiences with polyethylene ventilation tubes in children with recurrent middle ear inflammation (author's transl)]. Middle-ear ventilation tubes (of the Sheehy modification) were placed in 401 ears of 209 children under 16 years of age as treatment for recurrent middle-ear infection. The same procedure was later required in 26% of the cases studied because of further recurrence of middle-ear infection. In four per cent of all patients studied, the ventilating tube failed to cure middle ear disease in spite of controlled concomitant antibacterial therapy. In these cases, an early extrusion of the tube was commonly found. Among all patients, the tube was spontaneously extruded in 76 per cent of the ears, whereas the intubation time was longer than half a year in 53 per cent of the cases. No complications from insertion of the ventilation tubes was seen in the present series of patients. The tympanic membrane was found to be normal in 71 per cent of all cases when follow-up examination was obtained three months after completion in intubation. However, a permanent tympanic membrane perforation was found in five ears (1% of patients). The present study has confirmed the usefulness of middle-ear ventilation tubes, particularly in patients with recurrent middle-ear infections.", "PMID": 955999}
{"id": "pubmed23n0032_1905", "title": "[A new pneumatic nasal tamponade (author's transl)].", "content": "In order to control severe bleeding in the nose and nasopharynx, a pneumatic nasal tamponade has been devised. The tube is available in three sizes, and can be easily introduced into the nasal cavity to sufficiently block bleeding areas.", "contents": "[A new pneumatic nasal tamponade (author's transl)]. In order to control severe bleeding in the nose and nasopharynx, a pneumatic nasal tamponade has been devised. The tube is available in three sizes, and can be easily introduced into the nasal cavity to sufficiently block bleeding areas.", "PMID": 956000}
{"id": "pubmed23n0032_1906", "title": "[Oral mucosal grafts in laryngeal reconstruction (author's transl)].", "content": "In many laryngofissure procedures large mucosal resections are necessary. Free grafts of oral mucosa have been used in over 50 patients over the past 3 years for glottic reconstruction, and these experiences are reported in detail. Its use in the management of papillomata of the adult larynx is stressed.", "contents": "[Oral mucosal grafts in laryngeal reconstruction (author's transl)]. In many laryngofissure procedures large mucosal resections are necessary. Free grafts of oral mucosa have been used in over 50 patients over the past 3 years for glottic reconstruction, and these experiences are reported in detail. Its use in the management of papillomata of the adult larynx is stressed.", "PMID": 956001}
{"id": "pubmed23n0032_1907", "title": "Evaluation of the effects of presentation rate and speaking rate on children's auditory perception of two levels of linguistic material.", "content": "Twenty-four first- and second-order sentential approximations of four words each were presented auditorily to two groups of eight normal children. Eight first- and eight second-order approximations were spoken at three rates: 90 (slow); 140 (normal); and 175 (fast) words/min. The 48 approximations were randomized and rates altered as necessary so that one group heard the material at a slow rate (90 words/min) of presentation and the other group at a normal rate (140 words/min) of presentation. The results indicated that although presentation rate did not significantly effect auditory perception, speaking rate did. It was concluded that a slow speaking rate can be expected to enhance the perception of low-level linguistic material regardless of the presentation rate, whereas a normal speaking rate would be best for higher level linguistic material.", "contents": "Evaluation of the effects of presentation rate and speaking rate on children's auditory perception of two levels of linguistic material. Twenty-four first- and second-order sentential approximations of four words each were presented auditorily to two groups of eight normal children. Eight first- and eight second-order approximations were spoken at three rates: 90 (slow); 140 (normal); and 175 (fast) words/min. The 48 approximations were randomized and rates altered as necessary so that one group heard the material at a slow rate (90 words/min) of presentation and the other group at a normal rate (140 words/min) of presentation. The results indicated that although presentation rate did not significantly effect auditory perception, speaking rate did. It was concluded that a slow speaking rate can be expected to enhance the perception of low-level linguistic material regardless of the presentation rate, whereas a normal speaking rate would be best for higher level linguistic material.", "PMID": 956002}
{"id": "pubmed23n0032_1908", "title": "Development of absolute auditory thresholds in the house mouse (Mus musculus).", "content": "The development of hearing in the house mouse (Mus musculus) was behaviorally tested from the first postnatally measurable thresholds to those of young adults. An unconditioned stop reaction on tones was used in 9- to 11-day-old mice, then an unconditioned pinna reflex that can be elicited at low intensities and is not equal to the Preyer reflex. In addition, thresholds from 17- to 19-day-old and adult mice were obtained by a conditioned eyelid reflex. Frequencies between 1 and 80 kHz were tested. First reactions on tones were found at day 10 after birth in the frequency range of greatest sensitivity in the adults (10-20 kHz). The sensitivity optimum at 15 kHz becomes evident at day 14. Up to day 15 a sensitivity increase is noticeable for frequencies below the optimum, whereas high frequency sensitivity increases up to day 18. A second maximum of sensitivity at 50 kHz is measurable in 2- to 3-month-old mice, the threshold curve of which is defined as the standard for Mus musculus. The present behavioral data can well be correlated with existing electrophysiological results in mice and rats.", "contents": "Development of absolute auditory thresholds in the house mouse (Mus musculus). The development of hearing in the house mouse (Mus musculus) was behaviorally tested from the first postnatally measurable thresholds to those of young adults. An unconditioned stop reaction on tones was used in 9- to 11-day-old mice, then an unconditioned pinna reflex that can be elicited at low intensities and is not equal to the Preyer reflex. In addition, thresholds from 17- to 19-day-old and adult mice were obtained by a conditioned eyelid reflex. Frequencies between 1 and 80 kHz were tested. First reactions on tones were found at day 10 after birth in the frequency range of greatest sensitivity in the adults (10-20 kHz). The sensitivity optimum at 15 kHz becomes evident at day 14. Up to day 15 a sensitivity increase is noticeable for frequencies below the optimum, whereas high frequency sensitivity increases up to day 18. A second maximum of sensitivity at 50 kHz is measurable in 2- to 3-month-old mice, the threshold curve of which is defined as the standard for Mus musculus. The present behavioral data can well be correlated with existing electrophysiological results in mice and rats.", "PMID": 956003}
{"id": "pubmed23n0032_1909", "title": "Idiosyncratic cardiovascular response of human neonates to acoustic stimuli.", "content": "The heart rate response of acoustic stimuli was assssed for 15 newborns between the ages of 24 and 48 hr. Results of the analyses of covariance demonstrated that although the subjects responded to the stimuli, their responses were idiosyncratic in magnitude, direction, and pattern of rate change. Although spectrum and duration of the stimuli were varied, they were found not to be significant sources of response variation.", "contents": "Idiosyncratic cardiovascular response of human neonates to acoustic stimuli. The heart rate response of acoustic stimuli was assssed for 15 newborns between the ages of 24 and 48 hr. Results of the analyses of covariance demonstrated that although the subjects responded to the stimuli, their responses were idiosyncratic in magnitude, direction, and pattern of rate change. Although spectrum and duration of the stimuli were varied, they were found not to be significant sources of response variation.", "PMID": 956004}
{"id": "pubmed23n0032_1910", "title": "The crab louse-review of physiology and study of anatomy as seen by the scanning electron microscope.", "content": "Effective control of a parasitic infestation is aided by a thorough understanding of the parasite and it interaction with the host. The external anatomy of Phthirus pubis and its egg were studied by scanning electron microscopy and the structures visualized were reviewed to determine their function. The pathophysiology of the insect-host interaction and current treatment recommendations were also reviewed.", "contents": "The crab louse-review of physiology and study of anatomy as seen by the scanning electron microscope. Effective control of a parasitic infestation is aided by a thorough understanding of the parasite and it interaction with the host. The external anatomy of Phthirus pubis and its egg were studied by scanning electron microscopy and the structures visualized were reviewed to determine their function. The pathophysiology of the insect-host interaction and current treatment recommendations were also reviewed.", "PMID": 956012}
{"id": "pubmed23n0032_1911", "title": "Admittance values associated with the acoustic reflex and reflex decay.", "content": "Acoustic stapedial reflexes were elicited from 10 normal S's in each of three age groups. Susceptance and conductance values were measured for each subject in both the unreflexed and reflexed states and at 10-sec intervals during a continuous, reflex-sustaining stimulation with pure tones. Percentage of reflex decay over time was noted for each group and as a function of stimulus frequency and measurement mode. No significant age group differences were noted. Significant stimulus frequency and measurement mode differences were presented to represent \"normal\" reflex decay.", "contents": "Admittance values associated with the acoustic reflex and reflex decay. Acoustic stapedial reflexes were elicited from 10 normal S's in each of three age groups. Susceptance and conductance values were measured for each subject in both the unreflexed and reflexed states and at 10-sec intervals during a continuous, reflex-sustaining stimulation with pure tones. Percentage of reflex decay over time was noted for each group and as a function of stimulus frequency and measurement mode. No significant age group differences were noted. Significant stimulus frequency and measurement mode differences were presented to represent \"normal\" reflex decay.", "PMID": 956006}
{"id": "pubmed23n0032_1912", "title": "Pure tone delayed auditory feedback: development of criteria of performance deterioration.", "content": "Delayed auditory feedback audiometry, employing pure tones and a keytapping task, was administered at sensation levels from -10 to +50 dB to a sample of the general adult population. Absolute and relative time error as well as pattern error criteria were established on the basis of the -10-dB performances. Ninety percent of the subjects demonstrated tapping errors at sensation levels within 10 dB of auditory threshold.", "contents": "Pure tone delayed auditory feedback: development of criteria of performance deterioration. Delayed auditory feedback audiometry, employing pure tones and a keytapping task, was administered at sensation levels from -10 to +50 dB to a sample of the general adult population. Absolute and relative time error as well as pattern error criteria were established on the basis of the -10-dB performances. Ninety percent of the subjects demonstrated tapping errors at sensation levels within 10 dB of auditory threshold.", "PMID": 956005}
{"id": "pubmed23n0032_1913", "title": "An artifact in acoustic reflex measurement: some further observations.", "content": "This study was designed to clarify characteristics of an artifact that has been reported to be a potential source of misleading information when determing acoustic reflex thresholds elicited by low-frequency tones. The study was conducted in two parts. Experiment 1 was concerned with an investigation of acoustic interactions in a sealed cavity between the probe tone from a commercial electroacoustic impedance bridge and interfering tones of various frequencies. Experiment 2 consisted of measurements of sound pressure levels in the sealed external ear canal of the probe ear when the contralateral ear was stimulated by air-conducted pure tones. The results indicated that acoustic reflex thresholds in 250-Hz tones probably cannot be determined for most persons unless special measures are taken to prevent the artifact. The artifact should not occur when the eliciting stimulus is 500 Hz, however.", "contents": "An artifact in acoustic reflex measurement: some further observations. This study was designed to clarify characteristics of an artifact that has been reported to be a potential source of misleading information when determing acoustic reflex thresholds elicited by low-frequency tones. The study was conducted in two parts. Experiment 1 was concerned with an investigation of acoustic interactions in a sealed cavity between the probe tone from a commercial electroacoustic impedance bridge and interfering tones of various frequencies. Experiment 2 consisted of measurements of sound pressure levels in the sealed external ear canal of the probe ear when the contralateral ear was stimulated by air-conducted pure tones. The results indicated that acoustic reflex thresholds in 250-Hz tones probably cannot be determined for most persons unless special measures are taken to prevent the artifact. The artifact should not occur when the eliciting stimulus is 500 Hz, however.", "PMID": 956008}
{"id": "pubmed23n0032_1914", "title": "Minocycline in the treatment of lymphogranuloma venereum.", "content": "In a study of 80 cases of lymphogranuloma venereum (LGV), minocycline hydrochloride (Minocin) was found to be an effective drug in the treatment of all stages of LGV, including complicated ones. In late cases adjuvant treatment was used in addition to the antibiotic. Healing time in uncomplicated cases was less than 10 days. In complicated cases, both early and late, healing took about 2 to 3 weeks. Reactions to the drug were not significant.", "contents": "Minocycline in the treatment of lymphogranuloma venereum. In a study of 80 cases of lymphogranuloma venereum (LGV), minocycline hydrochloride (Minocin) was found to be an effective drug in the treatment of all stages of LGV, including complicated ones. In late cases adjuvant treatment was used in addition to the antibiotic. Healing time in uncomplicated cases was less than 10 days. In complicated cases, both early and late, healing took about 2 to 3 weeks. Reactions to the drug were not significant.", "PMID": 956013}
{"id": "pubmed23n0032_1915", "title": "Levamisole resinate as a Dirofilaria immitis microfilaricide in dogs.", "content": "Dogs infected naturally or experimentally with Dirofilaria immitis were given levamisole resinate at the rate of 5.5 or 11 mg/kg of body weight/day for 10 days. All dogs had been treated with thiacetarsamide sodium approximately 6 weeks earlier. When given at the dosage of 5.5 mg/kg for 10 days, levamisole resinate was not effective as a microfilaricide in either naturally or experimentally infected dogs. Microfilaria counts were reduced in all dogs at this dosage, but never to zero. In all cases where the higher dosage (11 mg/kg) was used, microfilaria counts were reduced to zero within 10 days. Signs of toxicosis were noticed in only 2 experimentally infected dogs, both of which were found to be harboring live adult male D immitis at necropsy.", "contents": "Levamisole resinate as a Dirofilaria immitis microfilaricide in dogs. Dogs infected naturally or experimentally with Dirofilaria immitis were given levamisole resinate at the rate of 5.5 or 11 mg/kg of body weight/day for 10 days. All dogs had been treated with thiacetarsamide sodium approximately 6 weeks earlier. When given at the dosage of 5.5 mg/kg for 10 days, levamisole resinate was not effective as a microfilaricide in either naturally or experimentally infected dogs. Microfilaria counts were reduced in all dogs at this dosage, but never to zero. In all cases where the higher dosage (11 mg/kg) was used, microfilaria counts were reduced to zero within 10 days. Signs of toxicosis were noticed in only 2 experimentally infected dogs, both of which were found to be harboring live adult male D immitis at necropsy.", "PMID": 956014}
{"id": "pubmed23n0032_1916", "title": "Lack of reliability of the SISI procedure for determining inner ear threshold.", "content": "Ten normally hearing subjects were tested using the conductive short increment sensitivity index procedure to evaluate the 60-dB constant as used in the test described by Byers (1974). Also investigated were the effects of frequency and order of presentation on the short increment sensitivity index at high sensation levels. Test frequencies included 1, 2, 4, and 6 kHz in random order. Means of all frequencies exceeded the 60-dB constant implemented by Byers (1974). Intersubject variability was large. Frequency and order of presentation were not statistically significant factors. It was recommended that the conductive short increment sensitivity index be used with caution.", "contents": "Lack of reliability of the SISI procedure for determining inner ear threshold. Ten normally hearing subjects were tested using the conductive short increment sensitivity index procedure to evaluate the 60-dB constant as used in the test described by Byers (1974). Also investigated were the effects of frequency and order of presentation on the short increment sensitivity index at high sensation levels. Test frequencies included 1, 2, 4, and 6 kHz in random order. Means of all frequencies exceeded the 60-dB constant implemented by Byers (1974). Intersubject variability was large. Frequency and order of presentation were not statistically significant factors. It was recommended that the conductive short increment sensitivity index be used with caution.", "PMID": 956007}
{"id": "pubmed23n0032_1917", "title": "Middle ear muscle activity during speech in stapedectomized and laryngectomized subjects.", "content": "Middle ear muscle responses associated with speech production were observed in normal-hearing, stapedectomized, and laryngectomized subjects. Impedance changes associated with speech production were monitored by an electroacoustic impedance bridge simultaneously with vocal output. Results from stapedectomized subjects indicate that the tensor tympani muscle contracts prior to vocalization and is part of the neurological pattern of speech production. Data collected from laryngectomized subjects suggest that the presence of sensory fibers from the larynx is not a prerequisite for middle ear muscle activity during speech production.", "contents": "Middle ear muscle activity during speech in stapedectomized and laryngectomized subjects. Middle ear muscle responses associated with speech production were observed in normal-hearing, stapedectomized, and laryngectomized subjects. Impedance changes associated with speech production were monitored by an electroacoustic impedance bridge simultaneously with vocal output. Results from stapedectomized subjects indicate that the tensor tympani muscle contracts prior to vocalization and is part of the neurological pattern of speech production. Data collected from laryngectomized subjects suggest that the presence of sensory fibers from the larynx is not a prerequisite for middle ear muscle activity during speech production.", "PMID": 956009}
{"id": "pubmed23n0032_1918", "title": "Northwestern University Auditory Test No. 6: normative and comparative intelligibility functions.", "content": "A commercially available recording of Northwestern University Auditory Test No. 6 (N.U. No. 6) produced by Auditec of St. Louis was evaluated in a series of four studies. Interlist equivalence of this version was initially investigated, and then normative intelligibility functions were developed using listerners with normal hearing. Intelligibility functions for the original version of N.U. No. 6. prepared by Northwestern University, were then derived, concurrently with functions of the Auditec version, using (1) a group of listeners with normal hearing; and (2) a group with sensorineural hearing loss. The results demonstrated good interlist equivalence for the Auditec version of N.U. No. 6. The comparative intelligibility functions for the Auditec and Northwestern versions of N.U. No 6 furthermore indicated a difference between the two recordings, but it was concluded that for clinical purposes the two versions may be considered equivalent.", "contents": "Northwestern University Auditory Test No. 6: normative and comparative intelligibility functions. A commercially available recording of Northwestern University Auditory Test No. 6 (N.U. No. 6) produced by Auditec of St. Louis was evaluated in a series of four studies. Interlist equivalence of this version was initially investigated, and then normative intelligibility functions were developed using listerners with normal hearing. Intelligibility functions for the original version of N.U. No. 6. prepared by Northwestern University, were then derived, concurrently with functions of the Auditec version, using (1) a group of listeners with normal hearing; and (2) a group with sensorineural hearing loss. The results demonstrated good interlist equivalence for the Auditec version of N.U. No. 6. The comparative intelligibility functions for the Auditec and Northwestern versions of N.U. No 6 furthermore indicated a difference between the two recordings, but it was concluded that for clinical purposes the two versions may be considered equivalent.", "PMID": 956010}
{"id": "pubmed23n0032_1919", "title": "Effects of signal processing on intelligibility of speech in noise for persons with sensorineural hearing loss.", "content": "A person with sensorineural hearing loss accompanied by recruitment has difficulty in understanding amplified speech, especially when speech is in the presence o competition. Two groups of subjects with sensorineural hearing loss with recruitment were tested at +6 and at O dB signal-to-noise (S/N) ratio at preferred levels for unprocessed speech and for speech processed through a two-band compression/equalization of circuit. In addition, unprocessed key word identification scores were compared to those from use of a hearing aid and of a one-band compression/equalization circuit. Processing a two-band compression/equalization circuit improved key word identification scores dramatically for both groups of subjects. The range of improvement for both groups was from 6 to 74%, mean 37.4%, for the S/N ratio of O dB. Subjects who achieved higher scores had high scores for unprocessed tests and had less recruitment present throughout the frequency range. Scores from one-band compression/equalization were compared to those from unprocessed scores; there were no significant differences in key word identification. Design characteristics and an excellent rationale for using two-band compression/equalization are generated by the data of this study.", "contents": "Effects of signal processing on intelligibility of speech in noise for persons with sensorineural hearing loss. A person with sensorineural hearing loss accompanied by recruitment has difficulty in understanding amplified speech, especially when speech is in the presence o competition. Two groups of subjects with sensorineural hearing loss with recruitment were tested at +6 and at O dB signal-to-noise (S/N) ratio at preferred levels for unprocessed speech and for speech processed through a two-band compression/equalization of circuit. In addition, unprocessed key word identification scores were compared to those from use of a hearing aid and of a one-band compression/equalization circuit. Processing a two-band compression/equalization circuit improved key word identification scores dramatically for both groups of subjects. The range of improvement for both groups was from 6 to 74%, mean 37.4%, for the S/N ratio of O dB. Subjects who achieved higher scores had high scores for unprocessed tests and had less recruitment present throughout the frequency range. Scores from one-band compression/equalization were compared to those from unprocessed scores; there were no significant differences in key word identification. Design characteristics and an excellent rationale for using two-band compression/equalization are generated by the data of this study.", "PMID": 956011}
{"id": "pubmed23n0032_1920", "title": "Basal cell tumors in dogs.", "content": "In a review of neoplasm registry records at Kansas State University (1961 through 1971), 140 basal cell tumors were reported in 32 breeds of dogs, representing 4.3% of 3,230 neoplasms recorded. Mean age of the affected dogs was 6.4 years. The tumors were removed surgically for biopsy in all dogs, 10 of which then had recurrences without metastasis, and required further surgery. The head, neck, and shoulder were a principal site (61%) of basal cell tumors in the dog.", "contents": "Basal cell tumors in dogs. In a review of neoplasm registry records at Kansas State University (1961 through 1971), 140 basal cell tumors were reported in 32 breeds of dogs, representing 4.3% of 3,230 neoplasms recorded. Mean age of the affected dogs was 6.4 years. The tumors were removed surgically for biopsy in all dogs, 10 of which then had recurrences without metastasis, and required further surgery. The head, neck, and shoulder were a principal site (61%) of basal cell tumors in the dog.", "PMID": 956018}
{"id": "pubmed23n0032_1921", "title": "Phenazopyridine toxicosis in the cat.", "content": "Severe illness developed after the oral administration of several drugs, including large doses of phenazopyridine (100 mg TID for 4 days) to a cat with dysuria and hematuria. Hemolysis and icterus were evident in blood serum and plasma after day 4 of drug administration, and many hemolyzed red blood cell \"ghosts\" containing Heinz bodies were observed on a stained blood smear. The cat became anemic and died within 48 hours after the last dose was administered. In an attempt to confirm a cause-and-effect relationship between drug administration and disease, 100 mg of phenazopyridine was given TID (65 mg/kg/day) for 3 days to a clinically normal cat. Nearly 50% of the hemoglobin was oxidized to methemoglobin during the course of phenazopyridine administration. Lower dosages of phenazopyridine (10 and 20 mg/kg/day) for longer periods of administration to 2 other clinically normal cats did not result in illness or anemia; however, the number and size of Heinz bodies and blood methemoglobin content were increased. Evidence of hepatic injury was observed in the clinically affected cat and in 2 of the experimental cats. The relationship between hepatic injury and toxic signs was not determined. Combination products recommeneded for treatment of cystitis in man often contain phenazopyridine. Such products should be avoided in cats unless a safe, effective dosage for phenazopyridine can be established.", "contents": "Phenazopyridine toxicosis in the cat. Severe illness developed after the oral administration of several drugs, including large doses of phenazopyridine (100 mg TID for 4 days) to a cat with dysuria and hematuria. Hemolysis and icterus were evident in blood serum and plasma after day 4 of drug administration, and many hemolyzed red blood cell \"ghosts\" containing Heinz bodies were observed on a stained blood smear. The cat became anemic and died within 48 hours after the last dose was administered. In an attempt to confirm a cause-and-effect relationship between drug administration and disease, 100 mg of phenazopyridine was given TID (65 mg/kg/day) for 3 days to a clinically normal cat. Nearly 50% of the hemoglobin was oxidized to methemoglobin during the course of phenazopyridine administration. Lower dosages of phenazopyridine (10 and 20 mg/kg/day) for longer periods of administration to 2 other clinically normal cats did not result in illness or anemia; however, the number and size of Heinz bodies and blood methemoglobin content were increased. Evidence of hepatic injury was observed in the clinically affected cat and in 2 of the experimental cats. The relationship between hepatic injury and toxic signs was not determined. Combination products recommeneded for treatment of cystitis in man often contain phenazopyridine. Such products should be avoided in cats unless a safe, effective dosage for phenazopyridine can be established.", "PMID": 956020}
{"id": "pubmed23n0032_1922", "title": "Cheyletiella infestation in cats.", "content": "While routinely examining a fecal specimen of a cat for evidence of intestinal parasitism, large eggs (230 by 110 mu) were observed. tfurther examination of the fecal specimen revealed an adult Cheyletiella sp. mite. Of 42 cats received from the same source, 11 had Cheyletiella mites, found either on the skin or in fecal specimens. Grossly visible skin changes were not observed in any of the cats. The conically shaped sensory organ on genu I of the mite resembled that of Cheyletiella blakei.", "contents": "Cheyletiella infestation in cats. While routinely examining a fecal specimen of a cat for evidence of intestinal parasitism, large eggs (230 by 110 mu) were observed. tfurther examination of the fecal specimen revealed an adult Cheyletiella sp. mite. Of 42 cats received from the same source, 11 had Cheyletiella mites, found either on the skin or in fecal specimens. Grossly visible skin changes were not observed in any of the cats. The conically shaped sensory organ on genu I of the mite resembled that of Cheyletiella blakei.", "PMID": 956021}
{"id": "pubmed23n0032_1923", "title": "Lissencephaly in two Lhasa Apso dogs.", "content": "Lissencephaly, a congenital absence of cerebrocortical convolutions, was discovered in 2 Lhasa Apso dogs by clinical and pathologic evaluation. Neurologic abnormalities, which became evident within the 1st year of life, included behavioral, visual, and convulsive disorders. The neurologic abnormalities were mild or delayed in onset after birth, indicating the dog is less dependent on the cerebral cortex for sensorimotor function than is man.", "contents": "Lissencephaly in two Lhasa Apso dogs. Lissencephaly, a congenital absence of cerebrocortical convolutions, was discovered in 2 Lhasa Apso dogs by clinical and pathologic evaluation. Neurologic abnormalities, which became evident within the 1st year of life, included behavioral, visual, and convulsive disorders. The neurologic abnormalities were mild or delayed in onset after birth, indicating the dog is less dependent on the cerebral cortex for sensorimotor function than is man.", "PMID": 956022}
{"id": "pubmed23n0032_1924", "title": "Naturally occurring Tyzzer's disease as a complication of distemper and mycotic pneumonia in a dog.", "content": "Tyzzer's disease, distemper, and mycotic pneumonia were diagnosed at necropsy of a 6-week-old mixed breed pup. The illness was characterized by lethargy, poor appetite, dysentery, and convulsions, the latter developing just prior to death. The necropsy findings included pale, dilated large intestine and swollen, dark liver. Histologically, multifocal hepatic necrosis, similar to that of Tyzzer's disease in other species of animals, was observed. Bacillus piliformis was found in hepatocytes and necrotic areas of the liver.", "contents": "Naturally occurring Tyzzer's disease as a complication of distemper and mycotic pneumonia in a dog. Tyzzer's disease, distemper, and mycotic pneumonia were diagnosed at necropsy of a 6-week-old mixed breed pup. The illness was characterized by lethargy, poor appetite, dysentery, and convulsions, the latter developing just prior to death. The necropsy findings included pale, dilated large intestine and swollen, dark liver. Histologically, multifocal hepatic necrosis, similar to that of Tyzzer's disease in other species of animals, was observed. Bacillus piliformis was found in hepatocytes and necrotic areas of the liver.", "PMID": 956024}
{"id": "pubmed23n0032_1925", "title": "Diseases of yearling feedlot cattle in Colorado.", "content": "During all of 1974 we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. The morbidity was 5.1%, with a case mortality of 18.9% and a population mortality of 1.0%. Both morbidity and mortality were higher during fall and winter than during spring and summer. Of the 3,943 dead cattle, 1,988 were necropsied. The most prevalent diseases were: pneumonia, 48%; diphtheria, 6%; brisket disease, 6%; hemorrhagic colitis, 5%; riding injury, 4%; bloat, 3%; calculosis, 2%; endocarditis, 2%; abomasal ulcers, 2%; bovine viral diarrhea, 2%; embolic pulmonary aneurysm, 1%; and pulmonary edema, 1%.", "contents": "Diseases of yearling feedlot cattle in Colorado. During all of 1974 we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. The morbidity was 5.1%, with a case mortality of 18.9% and a population mortality of 1.0%. Both morbidity and mortality were higher during fall and winter than during spring and summer. Of the 3,943 dead cattle, 1,988 were necropsied. The most prevalent diseases were: pneumonia, 48%; diphtheria, 6%; brisket disease, 6%; hemorrhagic colitis, 5%; riding injury, 4%; bloat, 3%; calculosis, 2%; endocarditis, 2%; abomasal ulcers, 2%; bovine viral diarrhea, 2%; embolic pulmonary aneurysm, 1%; and pulmonary edema, 1%.", "PMID": 956027}
{"id": "pubmed23n0032_1926", "title": "Atypical interstitial pneumonia in yearling feedlot cattle.", "content": "During each week of 1974, we surveyed, for illnesses and deaths, a constantly changing population of yearling feedlot cattle that, for the year, totaled 407,000 animals. From 3,943 fatalities, 1,988 necropsies were made; of this number, 106 (5.3%) had atypical interstitial pneumonia. The death rate was higher during summer and fall than during the other seasons and was evenly distributed throughout each of the 4 stages of fattening. Gross pulmonary lesions involved both lungs and were prominent throughout the caudal (diaphragmatic) lobes. Epithelialization, hemorrhage, fibrin, hyaline membranes, emphysema, bronchiolitis, and interstitial edema were common, whereas chromatin strands, bronchiolar edema, interstitial emphysema, and obliterating bronchiolitis were less common histopathologic features.", "contents": "Atypical interstitial pneumonia in yearling feedlot cattle. During each week of 1974, we surveyed, for illnesses and deaths, a constantly changing population of yearling feedlot cattle that, for the year, totaled 407,000 animals. From 3,943 fatalities, 1,988 necropsies were made; of this number, 106 (5.3%) had atypical interstitial pneumonia. The death rate was higher during summer and fall than during the other seasons and was evenly distributed throughout each of the 4 stages of fattening. Gross pulmonary lesions involved both lungs and were prominent throughout the caudal (diaphragmatic) lobes. Epithelialization, hemorrhage, fibrin, hyaline membranes, emphysema, bronchiolitis, and interstitial edema were common, whereas chromatin strands, bronchiolar edema, interstitial emphysema, and obliterating bronchiolitis were less common histopathologic features.", "PMID": 956028}
{"id": "pubmed23n0032_1927", "title": "Brisket disease in yearling feedlot cattle.", "content": "During all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle maintained at 1,600 m altitude. Of 1,988 cattle necropsied, 116 (5.8%) had brisket disease. The malady occurred during all seasons but was most common throughout fall and winter. The gross changes were hypertrophy and dilatation of the right ventricle and generalizaed passive congestion. The postulated causative factors were high genetic susceptibility, rapid growth rate, previous mountain grazing, and hypoventilation with airway hypoxia.", "contents": "Brisket disease in yearling feedlot cattle. During all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle maintained at 1,600 m altitude. Of 1,988 cattle necropsied, 116 (5.8%) had brisket disease. The malady occurred during all seasons but was most common throughout fall and winter. The gross changes were hypertrophy and dilatation of the right ventricle and generalizaed passive congestion. The postulated causative factors were high genetic susceptibility, rapid growth rate, previous mountain grazing, and hypoventilation with airway hypoxia.", "PMID": 956029}
{"id": "pubmed23n0032_1928", "title": "Bulling among yearling feedlot steers.", "content": "In a survey to determine the cause of illness and deaths among yearling feedlot cattle, bulling was found to be one of the major problems. During the years 1971-1974, 54,913 (2.88%) steers became bullers and represented an annual loss of around +325,000. Some of the causes of bulling were found to be hormones, either as implants or in the feed. In 1974, from 1,988 necropsies, it was determined that 83 steers died from riding injuries.", "contents": "Bulling among yearling feedlot steers. In a survey to determine the cause of illness and deaths among yearling feedlot cattle, bulling was found to be one of the major problems. During the years 1971-1974, 54,913 (2.88%) steers became bullers and represented an annual loss of around +325,000. Some of the causes of bulling were found to be hormones, either as implants or in the feed. In 1974, from 1,988 necropsies, it was determined that 83 steers died from riding injuries.", "PMID": 956030}
{"id": "pubmed23n0032_1929", "title": "Fetal abomasal ulcers in yearling feedlot cattle.", "content": "During all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. From the 3,943 dead cattle, 1,988 necropsies were made; of this number, 31 (1.6%) had ulcers with fatal perforations or hemorrhages; a similar additional number of necropsied cattle had innocuous ulcers as incidental findings. Ulcers developed during all seasons and all stages of fattening, but were more common during the first 45 days of winter-initiated fattening than during other times. Perforations of the abomasal wall resulted in spillage of abomasal contents into the abdominal cavity and the development of peritonitis.", "contents": "Fetal abomasal ulcers in yearling feedlot cattle. During all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. From the 3,943 dead cattle, 1,988 necropsies were made; of this number, 31 (1.6%) had ulcers with fatal perforations or hemorrhages; a similar additional number of necropsied cattle had innocuous ulcers as incidental findings. Ulcers developed during all seasons and all stages of fattening, but were more common during the first 45 days of winter-initiated fattening than during other times. Perforations of the abomasal wall resulted in spillage of abomasal contents into the abdominal cavity and the development of peritonitis.", "PMID": 956031}
{"id": "pubmed23n0032_1930", "title": "Sudden deaths in yearling feedlot cattle.", "content": "A survey of the causes for fatal diseases of yearling feedlot cattle was conducted on more than 407,000 cattle during a 14-month period. Of the 4,260 (1%) cattle that died during this period, 1,358 (32%) were categorized as cases of \"sudden death syndrome.\" Of the 11 most frequent causes of the syndrome, as determined at necropsy, only 4--bloat, pulmonary aneurysms, riding injury, and hemopericardium--were considered as short-course problems and true causes of sudden death. The largest number of cases of sudden death were attributed to pneumonia (113 animals). Consequently, the sudden death syndrome is a misnomer for many long-course diseases and, in some instances, a mask for neglect because, as clinically used, the name frequently includes cattle that have been sick, often with pneumonia, for several days.", "contents": "Sudden deaths in yearling feedlot cattle. A survey of the causes for fatal diseases of yearling feedlot cattle was conducted on more than 407,000 cattle during a 14-month period. Of the 4,260 (1%) cattle that died during this period, 1,358 (32%) were categorized as cases of \"sudden death syndrome.\" Of the 11 most frequent causes of the syndrome, as determined at necropsy, only 4--bloat, pulmonary aneurysms, riding injury, and hemopericardium--were considered as short-course problems and true causes of sudden death. The largest number of cases of sudden death were attributed to pneumonia (113 animals). Consequently, the sudden death syndrome is a misnomer for many long-course diseases and, in some instances, a mask for neglect because, as clinically used, the name frequently includes cattle that have been sick, often with pneumonia, for several days.", "PMID": 956032}
{"id": "pubmed23n0032_1931", "title": "Bronchiectasis in yearling feedlot cattle.", "content": "Thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. From the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. In this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including Pasteurella hemolytica, Pasteurella multocida, Corynebacterium pyogenes, Escherichia coli, Salmonella anatum, Staphylococcus spp, and Mycoplasma arginini.", "contents": "Bronchiectasis in yearling feedlot cattle. Thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. From the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. In this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including Pasteurella hemolytica, Pasteurella multocida, Corynebacterium pyogenes, Escherichia coli, Salmonella anatum, Staphylococcus spp, and Mycoplasma arginini.", "PMID": 956033}
{"id": "pubmed23n0032_1932", "title": "A new broad spectrum aminoglycoside antibiotic, G-52, produced by Micromonospora zionensis.", "content": "G-52 is a new broad spectrum aminoglycoside produced by a species of the genus Micromonospora, Micromonospora zionensis. It has been differentiated from other known related antibiotics by a variety of chemical and biological methods. Its in vitro and in vivo spectrum of activity appears to be quite similar to that of verdamicin and gentamicin but is differentiated from them by its increased activity against 6'-N-acetylating strains.", "contents": "A new broad spectrum aminoglycoside antibiotic, G-52, produced by Micromonospora zionensis. G-52 is a new broad spectrum aminoglycoside produced by a species of the genus Micromonospora, Micromonospora zionensis. It has been differentiated from other known related antibiotics by a variety of chemical and biological methods. Its in vitro and in vivo spectrum of activity appears to be quite similar to that of verdamicin and gentamicin but is differentiated from them by its increased activity against 6'-N-acetylating strains.", "PMID": 956034}
{"id": "pubmed23n0032_1933", "title": "The structure of antibiotic G-52, a new aminocyclitol-aminoglycoside antibiotic produced by Micromonospora zionensis.", "content": "Antibiotic G-52, a new aminocyclitol-aminoglycoside antibiotic produced in the fermentation of Micromonospora zionensis, has been shown to be 6'-N-methylsisomicin on the basis of its spectral characteristics. This assignment was confirmed by synthesis of the antibiotic from sisomicin.", "contents": "The structure of antibiotic G-52, a new aminocyclitol-aminoglycoside antibiotic produced by Micromonospora zionensis. Antibiotic G-52, a new aminocyclitol-aminoglycoside antibiotic produced in the fermentation of Micromonospora zionensis, has been shown to be 6'-N-methylsisomicin on the basis of its spectral characteristics. This assignment was confirmed by synthesis of the antibiotic from sisomicin.", "PMID": 956035}
{"id": "pubmed23n0032_1934", "title": "Nocardicin A, a new monocyclic beta-lactam antibiotic. I. Discovery, isolation and characterization.", "content": "Nocardicin A is a new monocyclic beta-lactam antibiotic obtained from the fermentation broth of a strain of actinomycetes. The producing organism, strain WS 1571, was identified as Nocardia uniformis subsp. tsuyamanensis ATCC 21806. The antibiotic, obtained as colorless crystals, exhibits moderate in vitro antibacterial activity against a broad-spectrum of Gram-negative bacteria including Proteus and Pseudomonas. It has low toxicity in laboratory animals.", "contents": "Nocardicin A, a new monocyclic beta-lactam antibiotic. I. Discovery, isolation and characterization. Nocardicin A is a new monocyclic beta-lactam antibiotic obtained from the fermentation broth of a strain of actinomycetes. The producing organism, strain WS 1571, was identified as Nocardia uniformis subsp. tsuyamanensis ATCC 21806. The antibiotic, obtained as colorless crystals, exhibits moderate in vitro antibacterial activity against a broad-spectrum of Gram-negative bacteria including Proteus and Pseudomonas. It has low toxicity in laboratory animals.", "PMID": 956036}
{"id": "pubmed23n0032_1935", "title": "Gardimycin, a new antibiotic from Actinoplanes. I. Description of the producer strain and fermentation studies.", "content": "Two strains of Actinoplanes have been isolated that produce a new peptide antibiotic named gardimycin. A detailed taxonomical study of such strains indicates that they differ between themselves and from all the described species of Actinoplanes. For this reason they are considered to be new species; for which the names A. garbadinensis nov. sp. and A. liguriae nov. sp. are proposed. The type strain of A. garbadinensis is A/10889(=ATCC 31049), the type strain of A. liguriae is A/6353 (=ATCC 31048). Studies on the medium and fermentation conditions are reported.", "contents": "Gardimycin, a new antibiotic from Actinoplanes. I. Description of the producer strain and fermentation studies. Two strains of Actinoplanes have been isolated that produce a new peptide antibiotic named gardimycin. A detailed taxonomical study of such strains indicates that they differ between themselves and from all the described species of Actinoplanes. For this reason they are considered to be new species; for which the names A. garbadinensis nov. sp. and A. liguriae nov. sp. are proposed. The type strain of A. garbadinensis is A/10889(=ATCC 31049), the type strain of A. liguriae is A/6353 (=ATCC 31048). Studies on the medium and fermentation conditions are reported.", "PMID": 956037}
{"id": "pubmed23n0032_1936", "title": "Gardimycin, a new antibiotic from Actinoplanes. II. Isolation and preliminary characterization.", "content": "The strain Actinoplanes garbadinensis nov. sp. produces a peptide antibiotic, named gardimycin, which is active in vitro and in vivo against Gram-positive bacteria. Isolation and purification of the product have been accomplished by extraction from the broth with butanol and dialysis of the crude extract, followed by counter-current distribution. Gardimycin is an open chain peptide with an approximate minimal formula C84H138N18S3-4O34Na. The following amino acids have been identified by column chromatography of an acid hydrolysate: serine, glutamic acid, alanine, leucine, isoleucine, glycine, valine and two sulphur-containing amino acids whose structure is presently under study. Tryptophan has been identified in an alkaline hydrolysate.", "contents": "Gardimycin, a new antibiotic from Actinoplanes. II. Isolation and preliminary characterization. The strain Actinoplanes garbadinensis nov. sp. produces a peptide antibiotic, named gardimycin, which is active in vitro and in vivo against Gram-positive bacteria. Isolation and purification of the product have been accomplished by extraction from the broth with butanol and dialysis of the crude extract, followed by counter-current distribution. Gardimycin is an open chain peptide with an approximate minimal formula C84H138N18S3-4O34Na. The following amino acids have been identified by column chromatography of an acid hydrolysate: serine, glutamic acid, alanine, leucine, isoleucine, glycine, valine and two sulphur-containing amino acids whose structure is presently under study. Tryptophan has been identified in an alkaline hydrolysate.", "PMID": 956038}
{"id": "pubmed23n0032_1937", "title": "Isolation of a new antibiotic 333-25, related to antibiotic EM 49. (Studies on antibiotics from the genus Bacillus. XI).", "content": "A new antibiotic, 333-25, active against Gram-positive and Gram-negative bacteria, was isolated from the culture broth of Bacillus circulans 333-25. The antibiotic is a basic acylpeptide containing 2,4-diaminobutyric acid (5), leucine (2), phenylalanine (1) and a fatty acid. It is closely related to antibiotic EM 49, but can be differentiated by chromatographic behaviour.", "contents": "Isolation of a new antibiotic 333-25, related to antibiotic EM 49. (Studies on antibiotics from the genus Bacillus. XI). A new antibiotic, 333-25, active against Gram-positive and Gram-negative bacteria, was isolated from the culture broth of Bacillus circulans 333-25. The antibiotic is a basic acylpeptide containing 2,4-diaminobutyric acid (5), leucine (2), phenylalanine (1) and a fatty acid. It is closely related to antibiotic EM 49, but can be differentiated by chromatographic behaviour.", "PMID": 956039}
{"id": "pubmed23n0032_1938", "title": "The constituent amino acids and fatty acid of antibiotic 333-25. (Studies on antibiotics from the genus Bacillus. XII.", "content": "Measurement of the optical rotational activities of the constituent amino acids of antibiotic 333-25 identified 2,4-diaminobutyric acid (D-form 1, L-form 4), L-leucine (2) and D-phenylalanine (1). The fatty acid constituent was determined to be beta-hydroxy anteisononanoic acid by gas chromatography, nuclear magnetic resonance and mass spectra. Differentiation from the structure of antibiotic EM 49 is discussed.", "contents": "The constituent amino acids and fatty acid of antibiotic 333-25. (Studies on antibiotics from the genus Bacillus. XII. Measurement of the optical rotational activities of the constituent amino acids of antibiotic 333-25 identified 2,4-diaminobutyric acid (D-form 1, L-form 4), L-leucine (2) and D-phenylalanine (1). The fatty acid constituent was determined to be beta-hydroxy anteisononanoic acid by gas chromatography, nuclear magnetic resonance and mass spectra. Differentiation from the structure of antibiotic EM 49 is discussed.", "PMID": 956040}
{"id": "pubmed23n0032_1939", "title": "A butyrylcholinesterase inhibitor produced by Penicillium sp. no. C-81 and its identity with mycelianamide.", "content": "A screening aimed at obtaining a cholinesterase inhibitor of microbial origin was carried out using Pseudomonas butyrylcholinesterase. A mycelium-extract of a fungus strain, belonging to the genus Penicillium, was found to produce such an enzyme inhibitor. The inhibitor was purified and crystallized as colorless leaflets. From physical and chemical studies, the inhibitor was identified as being identical with an antibiotic, mycelianamide, though this compound was not known to have enzyme inhibitor activity. The kinetics of the inhibition of Pseudomonas butyrylcholinesterase were also studied. Horse serum cholinesterase and hog liver carboxylesterase were also inhibited by the isolated Penicillium C-81 inhibitor, but lipase and acetylcholinesterase were not.", "contents": "A butyrylcholinesterase inhibitor produced by Penicillium sp. no. C-81 and its identity with mycelianamide. A screening aimed at obtaining a cholinesterase inhibitor of microbial origin was carried out using Pseudomonas butyrylcholinesterase. A mycelium-extract of a fungus strain, belonging to the genus Penicillium, was found to produce such an enzyme inhibitor. The inhibitor was purified and crystallized as colorless leaflets. From physical and chemical studies, the inhibitor was identified as being identical with an antibiotic, mycelianamide, though this compound was not known to have enzyme inhibitor activity. The kinetics of the inhibition of Pseudomonas butyrylcholinesterase were also studied. Horse serum cholinesterase and hog liver carboxylesterase were also inhibited by the isolated Penicillium C-81 inhibitor, but lipase and acetylcholinesterase were not.", "PMID": 956041}
{"id": "pubmed23n0032_1940", "title": "Modifications of a macrolide antibiotic midecamycin (SF-837). I. Synthesis and structure of 9,3''-diacetylmidecamycin.", "content": "9,3''-Diacetylmidecamycin (12) was synthesized from 4''-depropionyl-9,2',4''-triacetylmidecamycin (8) by heating the latter with propionic anhydride in pyridine followed by removal of 2'-acetyl group, with or without 18-enolpropionyl group. Direct acetylation of midecamycin (1) led to the formation of the 3'',4''-positional isomer (6). The structure of 12 was determined by mass, NMR and chemical degradation. The location of 3''-acetyl group was shown by the stereospecific 3 leads to 1 acetyl migration catalyzed by a base of 3-O-acetyl-4-O-propionyl-L-mycarose (13), and comparison of NMR and mass fragmentation with the 3,4-positional isomer (15). The latter's structure was independently supported by the nuclear Overhauser effect between methyl and propionyl group at C-3. The intramolecular 4 leads to 3 acyl shift that was taken place in the forced acylation of the mycarose moiety was found to be affected by the anomeric configuration, nature of aglycones and reaction temperature. Reverse 3 leads to 4 acyl migration occurred in acidic hydrolysis.", "contents": "Modifications of a macrolide antibiotic midecamycin (SF-837). I. Synthesis and structure of 9,3''-diacetylmidecamycin. 9,3''-Diacetylmidecamycin (12) was synthesized from 4''-depropionyl-9,2',4''-triacetylmidecamycin (8) by heating the latter with propionic anhydride in pyridine followed by removal of 2'-acetyl group, with or without 18-enolpropionyl group. Direct acetylation of midecamycin (1) led to the formation of the 3'',4''-positional isomer (6). The structure of 12 was determined by mass, NMR and chemical degradation. The location of 3''-acetyl group was shown by the stereospecific 3 leads to 1 acetyl migration catalyzed by a base of 3-O-acetyl-4-O-propionyl-L-mycarose (13), and comparison of NMR and mass fragmentation with the 3,4-positional isomer (15). The latter's structure was independently supported by the nuclear Overhauser effect between methyl and propionyl group at C-3. The intramolecular 4 leads to 3 acyl shift that was taken place in the forced acylation of the mycarose moiety was found to be affected by the anomeric configuration, nature of aglycones and reaction temperature. Reverse 3 leads to 4 acyl migration occurred in acidic hydrolysis.", "PMID": 956042}
{"id": "pubmed23n0032_1941", "title": "High resolution mass spectra of malformin and related cyclic peptides.", "content": "A comparison of the high resolution mass spectra of synthetic and natural preparations of malformin provided new evidence for the revised structure: (see article): The interpretation of the mass spectra was aided by the examination of the spectra of the synthetic peptides, desthiomalformin and enantio-5-valine malformin.", "contents": "High resolution mass spectra of malformin and related cyclic peptides. A comparison of the high resolution mass spectra of synthetic and natural preparations of malformin provided new evidence for the revised structure: (see article): The interpretation of the mass spectra was aided by the examination of the spectra of the synthetic peptides, desthiomalformin and enantio-5-valine malformin.", "PMID": 956043}
{"id": "pubmed23n0032_1942", "title": "A new semisynthetic 7alpha-methoxycephalosporin, CS-1170: 7beta-((cyanomethyl)thio)acetamido)-7alpha-methoxy-3-((1-methyl-1H-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid.", "content": "The synthesis and antimicrobial activity of a new semisynthetic 7alpha-methoxycephalosporin,7beta [[(cyanomethyl)thiol]acetamidol]-7alpha-methoxy-3-[[(1-methyl-1H-tetrazol-5-yl)thio]-methyl]-3-cephem-4-carboxylic acid (CS-1170), are described. This compound shows interesting antibacterial activity when compared to cefoxitin and cephalothin.", "contents": "A new semisynthetic 7alpha-methoxycephalosporin, CS-1170: 7beta-((cyanomethyl)thio)acetamido)-7alpha-methoxy-3-((1-methyl-1H-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid. The synthesis and antimicrobial activity of a new semisynthetic 7alpha-methoxycephalosporin,7beta [[(cyanomethyl)thiol]acetamidol]-7alpha-methoxy-3-[[(1-methyl-1H-tetrazol-5-yl)thio]-methyl]-3-cephem-4-carboxylic acid (CS-1170), are described. This compound shows interesting antibacterial activity when compared to cefoxitin and cephalothin.", "PMID": 956044}
{"id": "pubmed23n0032_1943", "title": "3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100), a new semisynthetic cephalosporin. I. Comparative in vitro antibacterial activities of SCE-100 and cephalexin (CEX).", "content": "3-Deacetoxy-7(alpha-amino)-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100) has a potent antibacterial activity against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration of SCE-100 is 0.2 approximately 12.5 mug/ml against Gram-positive bacteria including penicillin G-resistant Staphylococcus aureus and 6.25 approximately 100 mug/ml against Gram-negative bacteria. SCE-100 inhibits clinically isolated strains of penicillin G-resistant S. aureus and ampicillin-resistant Escherichia coli, as well as the corresponding sensitive strains. The activity of SCE-100 is enhanced by decreasing the inoculum size and is only slightly influenced by medium pH, difference of medium composition and the addition of horse serum. SCE-100 has bactericidal activity against S. aureus and E. coli. There is a stepwise development of in vitro bacterial resistance to SCE-100 by serial transfer of organisms into a liquid medium containing SCE-100. Cross resistance was observed between SCE-100 and other tested cephalosporins.", "contents": "3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100), a new semisynthetic cephalosporin. I. Comparative in vitro antibacterial activities of SCE-100 and cephalexin (CEX). 3-Deacetoxy-7(alpha-amino)-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100) has a potent antibacterial activity against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration of SCE-100 is 0.2 approximately 12.5 mug/ml against Gram-positive bacteria including penicillin G-resistant Staphylococcus aureus and 6.25 approximately 100 mug/ml against Gram-negative bacteria. SCE-100 inhibits clinically isolated strains of penicillin G-resistant S. aureus and ampicillin-resistant Escherichia coli, as well as the corresponding sensitive strains. The activity of SCE-100 is enhanced by decreasing the inoculum size and is only slightly influenced by medium pH, difference of medium composition and the addition of horse serum. SCE-100 has bactericidal activity against S. aureus and E. coli. There is a stepwise development of in vitro bacterial resistance to SCE-100 by serial transfer of organisms into a liquid medium containing SCE-100. Cross resistance was observed between SCE-100 and other tested cephalosporins.", "PMID": 956045}
{"id": "pubmed23n0032_1944", "title": "3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100), a new semisynthetic cephalosporin. III. Comparative studies on absorption, distribution and excretion of SCE-100 and cephalexin (CEX) in laboratory animals.", "content": "3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetoamido) cephalosporanic acid (SCE-100) was compared to cephalexin with respect to absorption, tissue distribution, and urinary and biliary excretion in mice, rats, rabbits and dogs. The absorption of SCE-100 after oral administration is generally slower than that of cephalexin, but its disappearance from blood and tissues is delayed. The concentration of SCE-100 in the liver is distinctly higher than that of cephalexin. These properties of SCE-100 are most clearly manifested in rats. There is no significant difference in urinary excretion of the two cephalosporins in rabbits and dogs, while in rabbits, biliary excretion of SCE-100 is more intensive than that of cephalexin.", "contents": "3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetamido) cephalosporanic acid (SCE-100), a new semisynthetic cephalosporin. III. Comparative studies on absorption, distribution and excretion of SCE-100 and cephalexin (CEX) in laboratory animals. 3-Deacetoxy-7-(alpha-amino-1-cyclohexenylacetoamido) cephalosporanic acid (SCE-100) was compared to cephalexin with respect to absorption, tissue distribution, and urinary and biliary excretion in mice, rats, rabbits and dogs. The absorption of SCE-100 after oral administration is generally slower than that of cephalexin, but its disappearance from blood and tissues is delayed. The concentration of SCE-100 in the liver is distinctly higher than that of cephalexin. These properties of SCE-100 are most clearly manifested in rats. There is no significant difference in urinary excretion of the two cephalosporins in rabbits and dogs, while in rabbits, biliary excretion of SCE-100 is more intensive than that of cephalexin.", "PMID": 956046}
{"id": "pubmed23n0032_1945", "title": "Micromonospora-produced sisomicin components.", "content": "A sisomicin fermentation carried out in the presence of (methyl-14C)-L-methionine resulted in a crude mixture, composed of methyl-14C-labeled sisomicin as a major component; and two 4''-C-desmethylsisomicin (66-40B and 66-40D) isomer-like components, an unidentified component and a gentamicin A-like antibiotic as minor components. When (methyl-14C)-L-methionine was added in an early stage of the fermentation (24 hours), incorporation of methyl-14C-label into polar components (e.g., gentamicin A-like antibiotic) preceded that into sisomicin. Chromatographic evidence for the bioconversion of (methyl-14C)-gentamicin A to a radioactive sisomicin-like product (possibly (3''-N-methyl-14C)-sisomicin) was seen, when a Micromonospora blocked mutant was incubated in the presence of the former antibiotic.", "contents": "Micromonospora-produced sisomicin components. A sisomicin fermentation carried out in the presence of (methyl-14C)-L-methionine resulted in a crude mixture, composed of methyl-14C-labeled sisomicin as a major component; and two 4''-C-desmethylsisomicin (66-40B and 66-40D) isomer-like components, an unidentified component and a gentamicin A-like antibiotic as minor components. When (methyl-14C)-L-methionine was added in an early stage of the fermentation (24 hours), incorporation of methyl-14C-label into polar components (e.g., gentamicin A-like antibiotic) preceded that into sisomicin. Chromatographic evidence for the bioconversion of (methyl-14C)-gentamicin A to a radioactive sisomicin-like product (possibly (3''-N-methyl-14C)-sisomicin) was seen, when a Micromonospora blocked mutant was incubated in the presence of the former antibiotic.", "PMID": 956052}
{"id": "pubmed23n0032_1946", "title": "A new metabolite from Streptomyces hygroscopicus I. Fermentation and isolation.", "content": "A deoxypentulose has been isolated from the fermentations of a new soil isolate of Streptomyces hygroscopicus (UC-5601). It was found to inhibit weakly and specifically the growth of one strain of Mycobacterium avium (UC-159).", "contents": "A new metabolite from Streptomyces hygroscopicus I. Fermentation and isolation. A deoxypentulose has been isolated from the fermentations of a new soil isolate of Streptomyces hygroscopicus (UC-5601). It was found to inhibit weakly and specifically the growth of one strain of Mycobacterium avium (UC-159).", "PMID": 956053}
{"id": "pubmed23n0032_1947", "title": "A new metabolite from Streptomyces hygroscopicus II. Identification as 1-deoxy-D-threo-pentulose.", "content": "Mass spectrometric studies show this metabolite to be a 1-deoxy-pentulose. The sterochemistry was established by reduction to the corresponding 1-deoxy-D-pentitols.", "contents": "A new metabolite from Streptomyces hygroscopicus II. Identification as 1-deoxy-D-threo-pentulose. Mass spectrometric studies show this metabolite to be a 1-deoxy-pentulose. The sterochemistry was established by reduction to the corresponding 1-deoxy-D-pentitols.", "PMID": 956054}
{"id": "pubmed23n0032_1948", "title": "Alborixin, a new antibiotic ionophore: taxonomy, isolation and biological properties.", "content": "Alborixin is an ionophorous antibiotic of the nigericin group isolated from cultures of a strain of Streptomyces albus. It is active against Gram-postive bacteria and is coccidiostatic, but it is very toxic. The antibacterial principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named alborixin.", "contents": "Alborixin, a new antibiotic ionophore: taxonomy, isolation and biological properties. Alborixin is an ionophorous antibiotic of the nigericin group isolated from cultures of a strain of Streptomyces albus. It is active against Gram-postive bacteria and is coccidiostatic, but it is very toxic. The antibacterial principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named alborixin.", "PMID": 956055}
{"id": "pubmed23n0032_1949", "title": "Kalafungin. II. Chemical transformations and the absolute configuration.", "content": "The properties of number of kalafungin derivatives and analogues are consistent with the proposed structure. The absolute stereochemistry of the three active centers was determined to be RRR by optical rotatory dispersion comparisons.", "contents": "Kalafungin. II. Chemical transformations and the absolute configuration. The properties of number of kalafungin derivatives and analogues are consistent with the proposed structure. The absolute stereochemistry of the three active centers was determined to be RRR by optical rotatory dispersion comparisons.", "PMID": 956056}
{"id": "pubmed23n0032_1950", "title": "The C-13 NMR spectrum of novobiocin.", "content": "The carbons of novobiocin have been assigned to peaks in the CMR spectrum on the basis of chemical shifts and off-resonance experiments on novobiocin and related compounds.", "contents": "The C-13 NMR spectrum of novobiocin. The carbons of novobiocin have been assigned to peaks in the CMR spectrum on the basis of chemical shifts and off-resonance experiments on novobiocin and related compounds.", "PMID": 956057}
{"id": "pubmed23n0032_1951", "title": "Social-skills training to modify abusive verbal outbursts in adults.", "content": "Social-skills training was used to modify abusive verbal outbursts displayed by two adult psychiatric patients. Five target behaviors--looking, irrelevant comments, hostile comments, inappropriate requests, and appropriate requests were monitored during role-played situations. Social-skills training, consisting of behavior rehearsal with modelling, focused instructions, and feedback, was introduced in a multiple-baseline design across individuals. Training improved all target behaviors. The improved behavior generalized to: (1) novel scenes role-played with the original respondent, (2) training and novel scenes role-played with a different respondent, and (3) interpersonal situations on the hospital ward.", "contents": "Social-skills training to modify abusive verbal outbursts in adults. Social-skills training was used to modify abusive verbal outbursts displayed by two adult psychiatric patients. Five target behaviors--looking, irrelevant comments, hostile comments, inappropriate requests, and appropriate requests were monitored during role-played situations. Social-skills training, consisting of behavior rehearsal with modelling, focused instructions, and feedback, was introduced in a multiple-baseline design across individuals. Training improved all target behaviors. The improved behavior generalized to: (1) novel scenes role-played with the original respondent, (2) training and novel scenes role-played with a different respondent, and (3) interpersonal situations on the hospital ward.", "PMID": 956071}
{"id": "pubmed23n0032_1952", "title": "Prompts to increase attendance in a community mental-health center.", "content": "The effect of a brief appointment reminder for mental-health center applicants is reported. A standardized phone message was delivered by an administrative staff member one to three days before the appointment and a sequential experimental design alternating baseline and phone message conditions was used to assess the effects of the procedure. The no-show rate averages were 32%, 11%, 25%, and 14% respectively. The cost of the procedure was $162.00, which was totally recovered when only six appointments were kept. Since over 1000 patients were involved in the study, the return on the investment of +162.00 is considerable. Implications for this procedure are discussed for other community mental-health centers and for other community care-giving agencies.", "contents": "Prompts to increase attendance in a community mental-health center. The effect of a brief appointment reminder for mental-health center applicants is reported. A standardized phone message was delivered by an administrative staff member one to three days before the appointment and a sequential experimental design alternating baseline and phone message conditions was used to assess the effects of the procedure. The no-show rate averages were 32%, 11%, 25%, and 14% respectively. The cost of the procedure was $162.00, which was totally recovered when only six appointments were kept. Since over 1000 patients were involved in the study, the return on the investment of +162.00 is considerable. Implications for this procedure are discussed for other community mental-health centers and for other community care-giving agencies.", "PMID": 956072}
{"id": "pubmed23n0032_1953", "title": "Classical conditioning of decreases in human systolic blood pressure.", "content": "Decrease in human systolic blood pressure of 4.35 mm Hg (range: 0 to 12 mm Hg) were classically conditioned in normal and hypertensive subjects using a delayed conditioning paradigm in which a 30-sec auditory stimulus (the conditioned stimulus) was followed immediately by tilting the subject 15degrees head-down to elicit small decreases in blood pressure. Conditioning occurred within five trials. A control group demonstrated that sensitization of the reflex by repeated tilting could not account for the blood-pressure decreases associated with the conditioned stimulus in experimental subjects.", "contents": "Classical conditioning of decreases in human systolic blood pressure. Decrease in human systolic blood pressure of 4.35 mm Hg (range: 0 to 12 mm Hg) were classically conditioned in normal and hypertensive subjects using a delayed conditioning paradigm in which a 30-sec auditory stimulus (the conditioned stimulus) was followed immediately by tilting the subject 15degrees head-down to elicit small decreases in blood pressure. Conditioning occurred within five trials. A control group demonstrated that sensitization of the reflex by repeated tilting could not account for the blood-pressure decreases associated with the conditioned stimulus in experimental subjects.", "PMID": 956073}
{"id": "pubmed23n0032_1954", "title": "The effects of a self-instructional package on overactive preschool boys.", "content": "The effects of a self-instructional package on three overactive preschool boys were investigated using a multiple-baseline design across subjects. Behavioral observations of the three target subjects indicated transfer of training effects from the experimental tasks to the classroom. On-task behaviors increased dramatically concomitant with the introduction of the self-instructional package, and treatment gains were maintained 22.5 weeks after baseline was initiated. In addition, the use of an observer-expectancy control condition gave further credibility to the demonstration of a cusal relationship.", "contents": "The effects of a self-instructional package on overactive preschool boys. The effects of a self-instructional package on three overactive preschool boys were investigated using a multiple-baseline design across subjects. Behavioral observations of the three target subjects indicated transfer of training effects from the experimental tasks to the classroom. On-task behaviors increased dramatically concomitant with the introduction of the self-instructional package, and treatment gains were maintained 22.5 weeks after baseline was initiated. In addition, the use of an observer-expectancy control condition gave further credibility to the demonstration of a cusal relationship.", "PMID": 956074}
{"id": "pubmed23n0032_1955", "title": "The effects of token reinforcement on the behavior of delinquents in cottage settings.", "content": "A token economy designed to modify the behavior of 125 adolescent males committed to a state correctional institution was implemented in the boys' cottages, focusing on social behavior (peer interaction), rule following, and task completion. The program was sequentially introduced in a multiple-baseline design in three independent cottages; a fourth group served as a comparison cottage. Appropriate behavior increased when the token program was introduced in each cottage. Data were collected for 14 months. Thus, the long-term effects of initial behavior change were assessed.", "contents": "The effects of token reinforcement on the behavior of delinquents in cottage settings. A token economy designed to modify the behavior of 125 adolescent males committed to a state correctional institution was implemented in the boys' cottages, focusing on social behavior (peer interaction), rule following, and task completion. The program was sequentially introduced in a multiple-baseline design in three independent cottages; a fourth group served as a comparison cottage. Appropriate behavior increased when the token program was introduced in each cottage. Data were collected for 14 months. Thus, the long-term effects of initial behavior change were assessed.", "PMID": 956075}
{"id": "pubmed23n0032_1956", "title": "Sarcomere lengthening and tension drop in the latent period of isolated frog skeletal muscle fibers.", "content": "A laser diffraction technique has been developed for registering small changes in sarcomere length. The technique is capable of resolving changes as small as 0.2 A in isolated frog skeletal muscle fibers. The small sarcomere lengthening that accompanies the drop in tension in the latent period of contraction was investigated. We suggest this lengthening be named latency elongation (LE). The LE is present in a completely slack fiber and must, therefore, be caused by a forcible lengthening process. Furthermore, the LE is dependent on the existence of an overlap between thin and tick filaments. The rate of elongation and the time interval between stimulation and maximum elongation may vary along the fiber. The maximum elongation was 3-5 A per sarcomere. At any instant the drop in tension is a product of the sum of sarcomere lengthenings along the fiber and the slope stiffness of the series elasticity. The latency relaxation (LR) could be registered in the sarcomere length range from 2.2 mum to 3.6-3.7 mum. The amplitude went through a sharp maximum at 3.0-3.1 mum. In the sarcomere length range from 2.2 to 2.8 mum the delay from onset to maximum LR was nearly proportional to the distance from the Z-line to the overlap zone. A working hypothesis is presented. It is suggested that the LE is caused by a lengthening of the thin filaments.", "contents": "Sarcomere lengthening and tension drop in the latent period of isolated frog skeletal muscle fibers. A laser diffraction technique has been developed for registering small changes in sarcomere length. The technique is capable of resolving changes as small as 0.2 A in isolated frog skeletal muscle fibers. The small sarcomere lengthening that accompanies the drop in tension in the latent period of contraction was investigated. We suggest this lengthening be named latency elongation (LE). The LE is present in a completely slack fiber and must, therefore, be caused by a forcible lengthening process. Furthermore, the LE is dependent on the existence of an overlap between thin and tick filaments. The rate of elongation and the time interval between stimulation and maximum elongation may vary along the fiber. The maximum elongation was 3-5 A per sarcomere. At any instant the drop in tension is a product of the sum of sarcomere lengthenings along the fiber and the slope stiffness of the series elasticity. The latency relaxation (LR) could be registered in the sarcomere length range from 2.2 mum to 3.6-3.7 mum. The amplitude went through a sharp maximum at 3.0-3.1 mum. In the sarcomere length range from 2.2 to 2.8 mum the delay from onset to maximum LR was nearly proportional to the distance from the Z-line to the overlap zone. A working hypothesis is presented. It is suggested that the LE is caused by a lengthening of the thin filaments.", "PMID": 956088}
{"id": "pubmed23n0032_1957", "title": "Intracellular Na+, K+, and C1- activities in Balanus photoreceptors.", "content": "Ion-sensitive microelectrodes were used to measure intracellular activities (aix) of Na+, K+, and C-1 in Balanus photoreceptors. Average values of aiNa, aiK, and aiCl were 28 mM, 120 mM, and 65 mM, respectively. Equilibrium potentials calculated from these average values were: Na+ +64 mV, K+ - 77 mV, and and Cl- -42 mV; ther average value of the resting potential for all cells examined was -41 mV. Long exposure to intense illumination produced measurable increases in aiNa. Classical Na+ - K+ reciprocal dilution experiments were analyzed with and without observed changes in aiK. As aoK was increased, the membrane depolarized, and aiK increased. Better agreement was found between the membrane potential and the directly determined EK than expected from the standard relation between Em and aoK. The latter produced pNa:pK estimates of the resting photoreceptor membrane that were higher than estimates based on data from the ion electrodes. Generally, Em was more negative than EK as aoK was increased. This is consistent with a significant chloride permeability in the dark-adapted photoreceptor.", "contents": "Intracellular Na+, K+, and C1- activities in Balanus photoreceptors. Ion-sensitive microelectrodes were used to measure intracellular activities (aix) of Na+, K+, and C-1 in Balanus photoreceptors. Average values of aiNa, aiK, and aiCl were 28 mM, 120 mM, and 65 mM, respectively. Equilibrium potentials calculated from these average values were: Na+ +64 mV, K+ - 77 mV, and and Cl- -42 mV; ther average value of the resting potential for all cells examined was -41 mV. Long exposure to intense illumination produced measurable increases in aiNa. Classical Na+ - K+ reciprocal dilution experiments were analyzed with and without observed changes in aiK. As aoK was increased, the membrane depolarized, and aiK increased. Better agreement was found between the membrane potential and the directly determined EK than expected from the standard relation between Em and aoK. The latter produced pNa:pK estimates of the resting photoreceptor membrane that were higher than estimates based on data from the ion electrodes. Generally, Em was more negative than EK as aoK was increased. This is consistent with a significant chloride permeability in the dark-adapted photoreceptor.", "PMID": 956089}
{"id": "pubmed23n0032_1958", "title": "The permeability of the sodium channel in Myxicola to the alkali cations.", "content": "Relative permeabilities to the alkali cations were determined, from the reversal potential (VRev), for the Na channel of internally perfused voltage-clamped Myxicola giant axons. PLi/PNa and PK/PNa are 0.94 and 0.076, respectively. Rb and Cs are not measurably permeant. VRev vs. the internal Na activity was well described by the constant field equation over a 300-fold range of internal Na concentrations. In agreement with findings on squid axons, the PK/PNa was found to increase when the K content of the internal perfusate was reduced (equivalent per equivalent substitution with TMA). Internal Rb and Cs also decreased the PK/PNa. The order of effectiveness of internal K, Rb, and Cs in increasing the Na selectivity of the Na channel was Cs greater than Rb greater than or equal to K. External Li increases the PK/PNa but this may be due to the formation of LiF internally. It may be that substances do not have to traverse the channel in order to affect the selectivity filter. Evidence is presented which suggests that the selectivity of the Na channel may be higher for Na in intact as compared to perfused giant axons. It was concluded that the channel selectivity properities do not reflect only some fixed structural features of the channel, but the selectivity filter has a labile organization.", "contents": "The permeability of the sodium channel in Myxicola to the alkali cations. Relative permeabilities to the alkali cations were determined, from the reversal potential (VRev), for the Na channel of internally perfused voltage-clamped Myxicola giant axons. PLi/PNa and PK/PNa are 0.94 and 0.076, respectively. Rb and Cs are not measurably permeant. VRev vs. the internal Na activity was well described by the constant field equation over a 300-fold range of internal Na concentrations. In agreement with findings on squid axons, the PK/PNa was found to increase when the K content of the internal perfusate was reduced (equivalent per equivalent substitution with TMA). Internal Rb and Cs also decreased the PK/PNa. The order of effectiveness of internal K, Rb, and Cs in increasing the Na selectivity of the Na channel was Cs greater than Rb greater than or equal to K. External Li increases the PK/PNa but this may be due to the formation of LiF internally. It may be that substances do not have to traverse the channel in order to affect the selectivity filter. Evidence is presented which suggests that the selectivity of the Na channel may be higher for Na in intact as compared to perfused giant axons. It was concluded that the channel selectivity properities do not reflect only some fixed structural features of the channel, but the selectivity filter has a labile organization.", "PMID": 956090}
{"id": "pubmed23n0032_1959", "title": "Neural modification by paired sensory stimuli.", "content": "With repetitive stimulation of two sensory pathways which are intact within the isolated nervous system of Hermissenda, features of a cellular conditioning paradigm were identified. Type A photoreceptors, unlike type B photoreceptors, produce fewer impulses in response to light following temporally specific pairing of light stimuli with rotation stimuli. Type A photoreceptor impulse wave-forms are also specifically changed by such stimulus regimens. These findings can be explained, at least in part, by increased inhibition of type A cells by type B cells after stimulus pairing.", "contents": "Neural modification by paired sensory stimuli. With repetitive stimulation of two sensory pathways which are intact within the isolated nervous system of Hermissenda, features of a cellular conditioning paradigm were identified. Type A photoreceptors, unlike type B photoreceptors, produce fewer impulses in response to light following temporally specific pairing of light stimuli with rotation stimuli. Type A photoreceptor impulse wave-forms are also specifically changed by such stimulus regimens. These findings can be explained, at least in part, by increased inhibition of type A cells by type B cells after stimulus pairing.", "PMID": 956091}
{"id": "pubmed23n0032_1960", "title": "Age and sex differences in lung elasticity, and in closing capacity in nonsmokers.", "content": "Static volume-pressure characteristics of the human lung, closing capacity (CC), closing pressure, and subdivisions of lung volumes were measured in 66 adult nonsmokers, aged 24-58 yr. There were systemic differences between the sexes as well as with age. Young females had less elastic recoil at any lung volume than young males. However, males lost elastic recoil with age faster than females so that in the older age groups the recoil was similar. There were no significant changes in compliance over the volume range containing most values of CC in either males or females. By comparing the age regression of CC and of elastic recoil pressures at 40 and 50% TLC we conclude that the increase in CC with age in males was attributable almost entirely to loss of recoil. In females none of the increase in closing capacity with age was attributable to loss of recoil. By exclusion, it is probably attributable to a change in the intrinsic properties of small airways or an increase in the pleural pressure gradient with age.", "contents": "Age and sex differences in lung elasticity, and in closing capacity in nonsmokers. Static volume-pressure characteristics of the human lung, closing capacity (CC), closing pressure, and subdivisions of lung volumes were measured in 66 adult nonsmokers, aged 24-58 yr. There were systemic differences between the sexes as well as with age. Young females had less elastic recoil at any lung volume than young males. However, males lost elastic recoil with age faster than females so that in the older age groups the recoil was similar. There were no significant changes in compliance over the volume range containing most values of CC in either males or females. By comparing the age regression of CC and of elastic recoil pressures at 40 and 50% TLC we conclude that the increase in CC with age in males was attributable almost entirely to loss of recoil. In females none of the increase in closing capacity with age was attributable to loss of recoil. By exclusion, it is probably attributable to a change in the intrinsic properties of small airways or an increase in the pleural pressure gradient with age.", "PMID": 956092}
{"id": "pubmed23n0032_1961", "title": "Energetics of isometric exercise in man.", "content": "In isometric contractions of the plantar flexors (5-40% of maximal tension, Tmax), VO2 is linearly related to the force exerted, averaging 2 ml/min-kg of tension. At tension levels above 5% Tmax the lactic acid contribution to the overall energy demand is constant at about 20%. Therefore, up to at least 40% Tmax,-muscle blood flow is not totally occluded, though it is impaired even at low force levels. Below 5% Tmax no lactic acid (LA) accumulates in blood. The energy required for the development of the tension is linearly related to the force exerted up to 33% Tmax, thereafter increasing disproportionately. In the transition from rest to static contractions of the plantar and forearm flexors (30 and 40% Tmax, respectively) VO2 increases initially to 200% of the controls, leveling off later at 150%. During recovery, VO2 increases up to 200% of the initial resting level, due to the payment of a large O2 debt, decreasing then with a t 1/2 of about 30 s. The glycolytic component is relatively more important in isometric contractions of the forearm than of the plantar flexors. No LA accumulates in static contractions of the plantar flexors of 5-10 s duration interrupted by equal pauses.", "contents": "Energetics of isometric exercise in man. In isometric contractions of the plantar flexors (5-40% of maximal tension, Tmax), VO2 is linearly related to the force exerted, averaging 2 ml/min-kg of tension. At tension levels above 5% Tmax the lactic acid contribution to the overall energy demand is constant at about 20%. Therefore, up to at least 40% Tmax,-muscle blood flow is not totally occluded, though it is impaired even at low force levels. Below 5% Tmax no lactic acid (LA) accumulates in blood. The energy required for the development of the tension is linearly related to the force exerted up to 33% Tmax, thereafter increasing disproportionately. In the transition from rest to static contractions of the plantar and forearm flexors (30 and 40% Tmax, respectively) VO2 increases initially to 200% of the controls, leveling off later at 150%. During recovery, VO2 increases up to 200% of the initial resting level, due to the payment of a large O2 debt, decreasing then with a t 1/2 of about 30 s. The glycolytic component is relatively more important in isometric contractions of the forearm than of the plantar flexors. No LA accumulates in static contractions of the plantar flexors of 5-10 s duration interrupted by equal pauses.", "PMID": 956093}
{"id": "pubmed23n0032_1962", "title": "Cold-induced shivering in men with thermoneutral skin temperatures.", "content": "Twenty-two male Caucasians, aged 20-47 yr, were exposed in a cold room to air temperatures of -33 degrees C while lying in sleeping bags for 2 h. Skin and rectal temperatures as well as electromyographic activity of the chin, forearm, and thigh, were recorded. Shivering occurred in all the subjects, even though skin temperatures were maintained between 31 and 33 degrees C. It is suggested that a counter-current heat exchange occurs whereby the warm blood of the common carotid artery is cooled by cool venous blood in the jugular veins. This cooled arterial blood, in irrigating the hypothalamus, causes shivering.", "contents": "Cold-induced shivering in men with thermoneutral skin temperatures. Twenty-two male Caucasians, aged 20-47 yr, were exposed in a cold room to air temperatures of -33 degrees C while lying in sleeping bags for 2 h. Skin and rectal temperatures as well as electromyographic activity of the chin, forearm, and thigh, were recorded. Shivering occurred in all the subjects, even though skin temperatures were maintained between 31 and 33 degrees C. It is suggested that a counter-current heat exchange occurs whereby the warm blood of the common carotid artery is cooled by cool venous blood in the jugular veins. This cooled arterial blood, in irrigating the hypothalamus, causes shivering.", "PMID": 956094}
{"id": "pubmed23n0032_1963", "title": "Effect of adrenergic agents and their mode of action on mucociliary clearance in man.", "content": "The aerosolized adrenergic (ADR) agent, isoproterenol (ISO), was found markedly to accelerate mucus clearance within the human tracheobronchial tree. Mucus transport was measured by external gamma counting of aerosolized Fe2O3 particles deposited on the mucous membrane during inhalation. Aerosolized epinephrine (EPI), despite its alpha-ADR bronchial vasoconstrictor activity, increased mucus clearance to the same degree as did aerosolized ISO, with its beta-ADR bronchial vasodilator activity. The vehicle used for delivery of the ADR agents, i.e., a H2O aerosol by intermittent positive-pressure breathing (IPPB), itself increased mucus clearance slightly, but did not elicit the enormous increases produced by the ADR agents. Parenteral ISO caused increases in clearances similar to aerosolized ISO and EPI (consistent with different tissue drug levels achieved). Oral atropine delayed clearance, but atropine prior to an ADR aerosol did not alter the mucus transport effect of the ADR agent. ISO and atropine, despite opposite effects on mucus clearance, caused equal bronchodilation. Thus, increased clearance following aerosolized ISO was not dependent on bronchial vasodilation, aqueous aerosol droplets, reflex parasympathetic activation, or bronchodilation, and seems best attributable to increased ciliary beat rate.", "contents": "Effect of adrenergic agents and their mode of action on mucociliary clearance in man. The aerosolized adrenergic (ADR) agent, isoproterenol (ISO), was found markedly to accelerate mucus clearance within the human tracheobronchial tree. Mucus transport was measured by external gamma counting of aerosolized Fe2O3 particles deposited on the mucous membrane during inhalation. Aerosolized epinephrine (EPI), despite its alpha-ADR bronchial vasoconstrictor activity, increased mucus clearance to the same degree as did aerosolized ISO, with its beta-ADR bronchial vasodilator activity. The vehicle used for delivery of the ADR agents, i.e., a H2O aerosol by intermittent positive-pressure breathing (IPPB), itself increased mucus clearance slightly, but did not elicit the enormous increases produced by the ADR agents. Parenteral ISO caused increases in clearances similar to aerosolized ISO and EPI (consistent with different tissue drug levels achieved). Oral atropine delayed clearance, but atropine prior to an ADR aerosol did not alter the mucus transport effect of the ADR agent. ISO and atropine, despite opposite effects on mucus clearance, caused equal bronchodilation. Thus, increased clearance following aerosolized ISO was not dependent on bronchial vasodilation, aqueous aerosol droplets, reflex parasympathetic activation, or bronchodilation, and seems best attributable to increased ciliary beat rate.", "PMID": 956095}
{"id": "pubmed23n0032_1964", "title": "Effect of volume history on successive partial expiratory flow-volume maneuvers.", "content": "In normal subjects, the second of two successive partial expiratory flow-volume (PEFV 2) curves often had higher isovolume maximal expiratory flow rates (Vmax) than the first (PEFV 1) (mean increase 30.2 +/- 13%). The higher Vmax on PEFV 2 was present only when there was a greater lung elastic recoil pressure (Pst(L)). In eight subjects the Pst(L) derived from sequential partial quasi-static pressure-volume curves, from interruption of the flow-volume maneuvers and at the start of the PEFV curves showed that isovolume upstream resistance increased although Vmax also increased after going to residual volume (RV). In four subjects the RV volume history did not change the pressure flow relationship across the upstream airways. If airways dimensions were the sole determinant of Vmax, then Vmax on PEFV 2 would be the same or smaller than on PEFV 1. That the opposite was observed in our study indicates that the increase in Pst(L), which results from parenchymal hysteresis, offsets any dimensional decrease in upstream airways due to airways hysteresis.", "contents": "Effect of volume history on successive partial expiratory flow-volume maneuvers. In normal subjects, the second of two successive partial expiratory flow-volume (PEFV 2) curves often had higher isovolume maximal expiratory flow rates (Vmax) than the first (PEFV 1) (mean increase 30.2 +/- 13%). The higher Vmax on PEFV 2 was present only when there was a greater lung elastic recoil pressure (Pst(L)). In eight subjects the Pst(L) derived from sequential partial quasi-static pressure-volume curves, from interruption of the flow-volume maneuvers and at the start of the PEFV curves showed that isovolume upstream resistance increased although Vmax also increased after going to residual volume (RV). In four subjects the RV volume history did not change the pressure flow relationship across the upstream airways. If airways dimensions were the sole determinant of Vmax, then Vmax on PEFV 2 would be the same or smaller than on PEFV 1. That the opposite was observed in our study indicates that the increase in Pst(L), which results from parenchymal hysteresis, offsets any dimensional decrease in upstream airways due to airways hysteresis.", "PMID": 956096}
{"id": "pubmed23n0032_1965", "title": "Respiratory, circulatory, and ECG changes during acute exposure to high altitude.", "content": "Quantitative electrocardiographic and accessory circulatory and respiratory mass spectrographic and accessory circulatory and respiratory mass spectrographic studies were carried out on 30 young, healthy subjects at rest during moderately acute, standardized stepwise exposure to simulated altitude up to 7,000 m (PAO2 26.9 mmHg). The ECG (Einthoven and Wilson leads) and the respiratory gases were recorded synchronously during ascent and descent. The extensive data assembled, summarized in six tables and illustrated in seven graphs, represent the basis for future investigations on the single factors which underlie hypoxia-induced ECG changes during exposure to high altitude.", "contents": "Respiratory, circulatory, and ECG changes during acute exposure to high altitude. Quantitative electrocardiographic and accessory circulatory and respiratory mass spectrographic and accessory circulatory and respiratory mass spectrographic studies were carried out on 30 young, healthy subjects at rest during moderately acute, standardized stepwise exposure to simulated altitude up to 7,000 m (PAO2 26.9 mmHg). The ECG (Einthoven and Wilson leads) and the respiratory gases were recorded synchronously during ascent and descent. The extensive data assembled, summarized in six tables and illustrated in seven graphs, represent the basis for future investigations on the single factors which underlie hypoxia-induced ECG changes during exposure to high altitude.", "PMID": 956097}
{"id": "pubmed23n0032_1966", "title": "Papain inhibition by serum.", "content": "Sera from seven animal species (rat, cow, cat, dog, human, rabbit, and hamster) were tested and found to inhibit the papain-catalyzed hydrolysis of alpha-N-benzoyl-L-arginine-p-nitroaniline-HCl (L-BAPA). The relative concentration of inhibitor in each serum sample was expressed in terms of its papain inhibitory capacity (PIC) defined as the number of units of papain inhibited per ml of serum. Rat serum contained the highest concentration of inhibitor while hamster serum contained the lowest concentration. The inhibitor appears to be competitive with respect to L-BAPA and is heat labile and nondialyzable. Antipapain activity can be separated from antitrypsin activity by (NH4)2SO4 fractionation.", "contents": "Papain inhibition by serum. Sera from seven animal species (rat, cow, cat, dog, human, rabbit, and hamster) were tested and found to inhibit the papain-catalyzed hydrolysis of alpha-N-benzoyl-L-arginine-p-nitroaniline-HCl (L-BAPA). The relative concentration of inhibitor in each serum sample was expressed in terms of its papain inhibitory capacity (PIC) defined as the number of units of papain inhibited per ml of serum. Rat serum contained the highest concentration of inhibitor while hamster serum contained the lowest concentration. The inhibitor appears to be competitive with respect to L-BAPA and is heat labile and nondialyzable. Antipapain activity can be separated from antitrypsin activity by (NH4)2SO4 fractionation.", "PMID": 956098}
{"id": "pubmed23n0032_1967", "title": "PO2 in the carotid body perfused and/or superfused with cell-free media.", "content": "We measured with a micro-O2 electrode the tissue PO2 (PtO2) in the cat carotid body (CB) to see whether it was adequately oxygenated when perfused or superfused with artificial, cell-free (c-f) solutions (pH = 7.4; temp = 35-38 degrees C). To obtain a relative measure of O2 consumption (VO2), we also measured the rate of disappearance of O2 following stoppage of the blood flow, and compared these disappearance curves with those during stoppage of the c-f perfusion solutions. In 14 cats normal (blood perfusion) PtO2 values ranged from 10 to 104 mmHG; chi = 72 +/- 4 (SE--as used throughout). During 3 h of c-f perfusion with air-equilibrated Locke's solution, PtO2 ranged from 62 to about 160 mmHg; chi = 133 +/- 4. When perfused with Fay's equilibrated with 98% O2-2% CO2 no PtO2 values in the CB were below 300 mmHg (4 cats). In eight additional cats the CB was cleared of blood then superfused with saline equilibrated with 50% O2 underneath and air-equilibrated saline over. Less than 5% of the PtO2 values found were below 5 mmHg. We conclude that most studies on the artificially perfused or superfused CB cannot be invalidated on the basis that the preparations were hypoxic. O2 disappearance curves taken during blood perfusion were significantly faster than during c-f perfusion indicating a marked reduction in VO2 with c-f perfusion.", "contents": "PO2 in the carotid body perfused and/or superfused with cell-free media. We measured with a micro-O2 electrode the tissue PO2 (PtO2) in the cat carotid body (CB) to see whether it was adequately oxygenated when perfused or superfused with artificial, cell-free (c-f) solutions (pH = 7.4; temp = 35-38 degrees C). To obtain a relative measure of O2 consumption (VO2), we also measured the rate of disappearance of O2 following stoppage of the blood flow, and compared these disappearance curves with those during stoppage of the c-f perfusion solutions. In 14 cats normal (blood perfusion) PtO2 values ranged from 10 to 104 mmHG; chi = 72 +/- 4 (SE--as used throughout). During 3 h of c-f perfusion with air-equilibrated Locke's solution, PtO2 ranged from 62 to about 160 mmHg; chi = 133 +/- 4. When perfused with Fay's equilibrated with 98% O2-2% CO2 no PtO2 values in the CB were below 300 mmHg (4 cats). In eight additional cats the CB was cleared of blood then superfused with saline equilibrated with 50% O2 underneath and air-equilibrated saline over. Less than 5% of the PtO2 values found were below 5 mmHg. We conclude that most studies on the artificially perfused or superfused CB cannot be invalidated on the basis that the preparations were hypoxic. O2 disappearance curves taken during blood perfusion were significantly faster than during c-f perfusion indicating a marked reduction in VO2 with c-f perfusion.", "PMID": 956099}
{"id": "pubmed23n0032_1968", "title": "Xenon and nitrogen single-breath washout curves in patients with airway obstruction.", "content": "In patients with chronic obstructive lung disease, we determined single-breath N2 and 133 Xe washout curves, and regional distributions of volumes (Vr) and of 133Xe boluses inhaled at residual volume (VIRV). Patients suffering from emphysema with minimal airway obstruction demonstrated large closing volumes and apicobasal distribution gradients, apparently because of a steep pulmonary recoil pressure-volume curve. In one subject with basal small airway disease there was no vertical gradient in regional residual volume; closing volume was increased with the 133Xe technique but almost absent with the N2 technique. Patients with moderate-to-severe airway obstruction had upward-sloping alveolar plateaus without distinct phase IV, and small apicobasal differences in Vr and VIRV. The latter resulted probably from increased regional differences in time constants counteracting the influence of gravity. Finally, patients with severe airway obstruction and basal emphysema demonstrated a rising N2 but a descending 133Xe plateau; the gradient for VIRV was normal, and reversed for Vr. This pattern was attributed to nongravitational differences in time constants causing a first in-first out distribution.", "contents": "Xenon and nitrogen single-breath washout curves in patients with airway obstruction. In patients with chronic obstructive lung disease, we determined single-breath N2 and 133 Xe washout curves, and regional distributions of volumes (Vr) and of 133Xe boluses inhaled at residual volume (VIRV). Patients suffering from emphysema with minimal airway obstruction demonstrated large closing volumes and apicobasal distribution gradients, apparently because of a steep pulmonary recoil pressure-volume curve. In one subject with basal small airway disease there was no vertical gradient in regional residual volume; closing volume was increased with the 133Xe technique but almost absent with the N2 technique. Patients with moderate-to-severe airway obstruction had upward-sloping alveolar plateaus without distinct phase IV, and small apicobasal differences in Vr and VIRV. The latter resulted probably from increased regional differences in time constants counteracting the influence of gravity. Finally, patients with severe airway obstruction and basal emphysema demonstrated a rising N2 but a descending 133Xe plateau; the gradient for VIRV was normal, and reversed for Vr. This pattern was attributed to nongravitational differences in time constants causing a first in-first out distribution.", "PMID": 956100}
{"id": "pubmed23n0032_1969", "title": "Maximal oxygen uptake during exercise with various combinations of arm and leg work.", "content": "Oxygen uptake (VO2) was determined in 10 males during the following types of maximal exercise (work time: about 5 min): uphill running, bicycling, arm work (cranking), and combined arm work and bicycling (A + L). The A + L exercise was performed in four different ways, the arms doing 10%, 20%, 30%, or 40% of the same total rate of work; and also with the maximal bicycle work load plus either maximal or submaximal arm work. VO2 was the same in running as in all types of A + L exercise, except when the arm work load was 10% and 40% of the total rate of work, where VO2 was 2.5% (P less than 0.05) and 9.4% (P less than 0.001) lower, respectively. Bicycle VO2 was lower than VO2 in running but equal to A + L VO2 when arm work intensity was 40% of the total rate of work. It is concluded that VO2 during maximal exercise a) to a certain extent depends on the exercising muscle mass, b) is lower than the oxygen-consuming potential of the muscles involved in A + L exercise, and c) in A + L exercise is influenced by the ratio of arm work to total rate of work and the subject's fitness for arm work and bicycling.", "contents": "Maximal oxygen uptake during exercise with various combinations of arm and leg work. Oxygen uptake (VO2) was determined in 10 males during the following types of maximal exercise (work time: about 5 min): uphill running, bicycling, arm work (cranking), and combined arm work and bicycling (A + L). The A + L exercise was performed in four different ways, the arms doing 10%, 20%, 30%, or 40% of the same total rate of work; and also with the maximal bicycle work load plus either maximal or submaximal arm work. VO2 was the same in running as in all types of A + L exercise, except when the arm work load was 10% and 40% of the total rate of work, where VO2 was 2.5% (P less than 0.05) and 9.4% (P less than 0.001) lower, respectively. Bicycle VO2 was lower than VO2 in running but equal to A + L VO2 when arm work intensity was 40% of the total rate of work. It is concluded that VO2 during maximal exercise a) to a certain extent depends on the exercising muscle mass, b) is lower than the oxygen-consuming potential of the muscles involved in A + L exercise, and c) in A + L exercise is influenced by the ratio of arm work to total rate of work and the subject's fitness for arm work and bicycling.", "PMID": 956101}
{"id": "pubmed23n0032_1970", "title": "Uteroplacental blood flow during hyperthermia with and without respiratory alkalosis.", "content": "The effect of induced maternal hyperthermia (1.5 degrees C rise over 60 min) on the uterine and umbilical circulations was examined in two groups of chronically instrumented pregnant sheep. Hyperventilation occurred in both groups. In the group in which the resultant respiratory alkalosis was untreated (N = 5), uterine blood flow decreased to 53 +/- 3% (mean +/- SE; P less than 0.01) of control at maximal maternal temperature. Umbilical blood flow also decreased 30 +/- 6% (P less than 0.01) below control levels. In the other group, normocapnia was maintained (N = 4). Uterine blood flow decreased in this group to 75% +/- 2% (P less than 0.01) of control levels, but umbilical blood flow did not significantly change. There was no significant change in maternal or fetal mean arterial pressure during hyperthermia in either group. Thus, maternal hyperthermia per se results in a significant decrease in uterine blood flow primarily through an effect on uterine vascular resistance, but without a concomitant change in umbilical blood flow.", "contents": "Uteroplacental blood flow during hyperthermia with and without respiratory alkalosis. The effect of induced maternal hyperthermia (1.5 degrees C rise over 60 min) on the uterine and umbilical circulations was examined in two groups of chronically instrumented pregnant sheep. Hyperventilation occurred in both groups. In the group in which the resultant respiratory alkalosis was untreated (N = 5), uterine blood flow decreased to 53 +/- 3% (mean +/- SE; P less than 0.01) of control at maximal maternal temperature. Umbilical blood flow also decreased 30 +/- 6% (P less than 0.01) below control levels. In the other group, normocapnia was maintained (N = 4). Uterine blood flow decreased in this group to 75% +/- 2% (P less than 0.01) of control levels, but umbilical blood flow did not significantly change. There was no significant change in maternal or fetal mean arterial pressure during hyperthermia in either group. Thus, maternal hyperthermia per se results in a significant decrease in uterine blood flow primarily through an effect on uterine vascular resistance, but without a concomitant change in umbilical blood flow.", "PMID": 956102}
{"id": "pubmed23n0032_1971", "title": "Effect of ascorbic acid on rate of heat acclimatization.", "content": "There is some indication in the literature that ascorbic acid (vitamin C) may reduce the physiological responses to heat stress. Consequently, the effect of ascorbic acid ingestion on heat-strain indicators has been studied on a group of 60 mining recruits undergoing climatic room acclimatization. Of the 60 men, 19 received a daily dose of 250 mg ascorbic acid; 21 a daily dose of 500 mg ascorbic acid; and 20 received a placebo daily. Measurements of rectal temperature, heart rate, and hourly sweat rate were made on all subjects during the 4 h of heat exposure per day for 10 days. The wet bulb temperature was 32.2 degrees C, the dry bulb 33.9 degrees C, the air movement 0.4 m/s, and the work rate 35 W. The results indicate that the rate and degree of acclimatization, as assessed by 4th-h rectal temperature, is enhanced by ascorbic acid supplementation and that no differences in response could be shown between daily dosages of 250 and 500 mg of vitamin C.", "contents": "Effect of ascorbic acid on rate of heat acclimatization. There is some indication in the literature that ascorbic acid (vitamin C) may reduce the physiological responses to heat stress. Consequently, the effect of ascorbic acid ingestion on heat-strain indicators has been studied on a group of 60 mining recruits undergoing climatic room acclimatization. Of the 60 men, 19 received a daily dose of 250 mg ascorbic acid; 21 a daily dose of 500 mg ascorbic acid; and 20 received a placebo daily. Measurements of rectal temperature, heart rate, and hourly sweat rate were made on all subjects during the 4 h of heat exposure per day for 10 days. The wet bulb temperature was 32.2 degrees C, the dry bulb 33.9 degrees C, the air movement 0.4 m/s, and the work rate 35 W. The results indicate that the rate and degree of acclimatization, as assessed by 4th-h rectal temperature, is enhanced by ascorbic acid supplementation and that no differences in response could be shown between daily dosages of 250 and 500 mg of vitamin C.", "PMID": 956103}
{"id": "pubmed23n0032_1972", "title": "Platelet antiserum inhibits hypoxic pulmonary vasoconstriction in the dog.", "content": "The literature suggests that platelets might help mediate the pulmonary vascular pressor response to hypoxia. This study evaluated the hypoxic response in dogs rendered acutely thrombocytopenic by the administration of platelet antiserum. Between 30 and 90 min after the antiserum the pulmonary vasoconstrictor response to hypoxia was virtually abolished, but subsequently returned at a time when the number of circulating platelets remained very low. The prior administration of meclofenamate completely preserved the hypoxic response, though the platelet count still fell precipitously. We conclude that circulating platelets are not necessary for hypoxic vasoconstriction. It is possible that the reaction between platelets and antiserum evokes the synthesis of a dilator prostaglandin which might be responsible for the temporary inhibition of the pressor response to hypoxia but this remains to be proven.", "contents": "Platelet antiserum inhibits hypoxic pulmonary vasoconstriction in the dog. The literature suggests that platelets might help mediate the pulmonary vascular pressor response to hypoxia. This study evaluated the hypoxic response in dogs rendered acutely thrombocytopenic by the administration of platelet antiserum. Between 30 and 90 min after the antiserum the pulmonary vasoconstrictor response to hypoxia was virtually abolished, but subsequently returned at a time when the number of circulating platelets remained very low. The prior administration of meclofenamate completely preserved the hypoxic response, though the platelet count still fell precipitously. We conclude that circulating platelets are not necessary for hypoxic vasoconstriction. It is possible that the reaction between platelets and antiserum evokes the synthesis of a dilator prostaglandin which might be responsible for the temporary inhibition of the pressor response to hypoxia but this remains to be proven.", "PMID": 956104}
{"id": "pubmed23n0032_1973", "title": "Influence of muscular development, obesity, and age on the fat-free mass of adults.", "content": "Body fat and the fat-free mass (FFM) were estimated in 36 men and 43 women deliberately chosen to represent a variety of physical types; these were 1) young sedentary, 2) \"muscular,\" 3) younger obese, 4) older obese, and 5) older nonobese individuals of both sexes. The body fat and the FFM were estimated from measurements of body density (by total immersion in water, measurement being made of the residual volume of air present in the lungs at immersion) and from measurements of total body potassium (using a whole-body monitor to assess the natural 40K isotope present in the body). The muscular men and women and the younger obese men and women had a considerably greater FFM and thus had greater quantities of potassium than the corresponding sedentary groups. There were significantly different estimates of the FFM calculated from density and from total body K in three groups, the sedentary young men, the muscular, and the younger obese women. The density and the potassium content of the FFM appear to decline with obesity and aging. Muscular development is associated with a decrease in the density but an increase in the potassium content of the FFM.", "contents": "Influence of muscular development, obesity, and age on the fat-free mass of adults. Body fat and the fat-free mass (FFM) were estimated in 36 men and 43 women deliberately chosen to represent a variety of physical types; these were 1) young sedentary, 2) \"muscular,\" 3) younger obese, 4) older obese, and 5) older nonobese individuals of both sexes. The body fat and the FFM were estimated from measurements of body density (by total immersion in water, measurement being made of the residual volume of air present in the lungs at immersion) and from measurements of total body potassium (using a whole-body monitor to assess the natural 40K isotope present in the body). The muscular men and women and the younger obese men and women had a considerably greater FFM and thus had greater quantities of potassium than the corresponding sedentary groups. There were significantly different estimates of the FFM calculated from density and from total body K in three groups, the sedentary young men, the muscular, and the younger obese women. The density and the potassium content of the FFM appear to decline with obesity and aging. Muscular development is associated with a decrease in the density but an increase in the potassium content of the FFM.", "PMID": 956105}
{"id": "pubmed23n0032_1974", "title": "Effects of water immersion on renal hemodynamics in normal man.", "content": "Although previous studies have demonstrated that water immersion to the neck (NI) results in a significant natriuresis and diuresis, the mechanisms are incompletely delineated. Since recent studies have demonstrated that NI induces a marked increase in cardiac index, it is possible that, as a consequence, renal hemodynamics may be augmented markedly during NI thereby mediating the encountered renal changes. The present study was undertaken to delineate the effects of NI on renal plasma flow and glomerular filtration rate as assessed by the clearance of PAH (CPAH) and inulin (CIn), respectively. Nine normal male subjects were studied on two occasions, control and NI. The conditions of seated posture and time of day were identical. Immersion did not alter CIn or CPAH at a time when sodium excretion was increasing markedly. The constancy of CPAH during NI suggests that renal blood flow is unaltered and that the natriuresis of NI is mediated independently of alterations in overall renal perfusion. The sluggish decline of a naturiuresis during recovery is consistent with the presence of a humoral factor contributing to the encountered natriuresis.", "contents": "Effects of water immersion on renal hemodynamics in normal man. Although previous studies have demonstrated that water immersion to the neck (NI) results in a significant natriuresis and diuresis, the mechanisms are incompletely delineated. Since recent studies have demonstrated that NI induces a marked increase in cardiac index, it is possible that, as a consequence, renal hemodynamics may be augmented markedly during NI thereby mediating the encountered renal changes. The present study was undertaken to delineate the effects of NI on renal plasma flow and glomerular filtration rate as assessed by the clearance of PAH (CPAH) and inulin (CIn), respectively. Nine normal male subjects were studied on two occasions, control and NI. The conditions of seated posture and time of day were identical. Immersion did not alter CIn or CPAH at a time when sodium excretion was increasing markedly. The constancy of CPAH during NI suggests that renal blood flow is unaltered and that the natriuresis of NI is mediated independently of alterations in overall renal perfusion. The sluggish decline of a naturiuresis during recovery is consistent with the presence of a humoral factor contributing to the encountered natriuresis.", "PMID": 956106}
{"id": "pubmed23n0032_1975", "title": "Effect of gas physical properties and flow on lower pulmonary resistance.", "content": "We measured lower pulmonary resistance (Rlp) in eight dogs and three men breathing gas mixtures having different densities (p) and similar viscosities (mu). Rlp increased with gas density and with flow rate (V). In the dogs, these effects were not observed in lung segments subtended from 4-mm-ID bronchi; in more central airways, resistance varied approximately as (mup V)0.5. These results are compatible with Poiseuille flow in peripheral airways, and, in central airways, with flow resistance described by the equation of boundary layer growth. Rather than two discrete flow regimes, it is likely that flow patterns undergo a continual metamorphosis as Reynolds' numbers (Re) decrease between trachea and alveoli. Accordingly, the airways pressure-flow relationship is not described by any single fluid dynamics equation, but may be explained by the general equation, P = Kmu2-apa-1Va, where a reflects the proportion of inertial to viscous pressure losses and varies between 1 and 2 according to Re. Rohrer's equation described the observed pressure-flow relationships and predicted the change in Rlp with gas physical properties, suggesting a physical basis underlying this adequate mathematical description.", "contents": "Effect of gas physical properties and flow on lower pulmonary resistance. We measured lower pulmonary resistance (Rlp) in eight dogs and three men breathing gas mixtures having different densities (p) and similar viscosities (mu). Rlp increased with gas density and with flow rate (V). In the dogs, these effects were not observed in lung segments subtended from 4-mm-ID bronchi; in more central airways, resistance varied approximately as (mup V)0.5. These results are compatible with Poiseuille flow in peripheral airways, and, in central airways, with flow resistance described by the equation of boundary layer growth. Rather than two discrete flow regimes, it is likely that flow patterns undergo a continual metamorphosis as Reynolds' numbers (Re) decrease between trachea and alveoli. Accordingly, the airways pressure-flow relationship is not described by any single fluid dynamics equation, but may be explained by the general equation, P = Kmu2-apa-1Va, where a reflects the proportion of inertial to viscous pressure losses and varies between 1 and 2 according to Re. Rohrer's equation described the observed pressure-flow relationships and predicted the change in Rlp with gas physical properties, suggesting a physical basis underlying this adequate mathematical description.", "PMID": 956107}
{"id": "pubmed23n0032_1976", "title": "Tissue oxygen tension in rabbits measured with a galvanic electrode.", "content": "A galvanic electrode which generates current in response to oxygen was used to measure tissue PO2 in 10 rabbits. Sixteen electrodes were calibrated in vitro at PO2 = 0 mmHg and PO2 = 100 mmHg before implantation into muscle and peritoneal cavity. Electrode calibration was checked after removal 7-20 days later; mean +/- SE reading at PO2 = 0 was 1.5 +/- 1.83 mmHg and at PO2 = 100 was 98.1 +/- 6.49 mmHg. Continuous recordings of tissue PO2 showed little fluctuation in unanesthetized rabbits resting quietly. Tissue PO2 was lower than arterial or venous PO2 during air breathing but frequently exceeded venous PO2 during O2 breathing. Highly significant correlations (P less than 0.001) were found between blood and tissue PO2 over a wide range of values obtained during air breathing and during 10%, 50%, and 100% O2 breathing. Thus, the galvanic electrode provided a reliable measure of tissue PO2 and maintained its stability in vivo for periods as long as 20 days.", "contents": "Tissue oxygen tension in rabbits measured with a galvanic electrode. A galvanic electrode which generates current in response to oxygen was used to measure tissue PO2 in 10 rabbits. Sixteen electrodes were calibrated in vitro at PO2 = 0 mmHg and PO2 = 100 mmHg before implantation into muscle and peritoneal cavity. Electrode calibration was checked after removal 7-20 days later; mean +/- SE reading at PO2 = 0 was 1.5 +/- 1.83 mmHg and at PO2 = 100 was 98.1 +/- 6.49 mmHg. Continuous recordings of tissue PO2 showed little fluctuation in unanesthetized rabbits resting quietly. Tissue PO2 was lower than arterial or venous PO2 during air breathing but frequently exceeded venous PO2 during O2 breathing. Highly significant correlations (P less than 0.001) were found between blood and tissue PO2 over a wide range of values obtained during air breathing and during 10%, 50%, and 100% O2 breathing. Thus, the galvanic electrode provided a reliable measure of tissue PO2 and maintained its stability in vivo for periods as long as 20 days.", "PMID": 956108}
{"id": "pubmed23n0032_1977", "title": "Correction for cardiac output calculations by the nitrous oxide technique.", "content": "Calculations of cardiac output using the N2O technique as described by Becklake et. al. (J. Appl. Physiol. 17: 579, 1962) result in spurious values when barometric pressure or body temperature deviates from 760 Torr or 37.0 degrees C, respectively. Nitrous oxide (N2O) uptake (VN2O) must be corrected to standard pressure, dry at 37.0 degrees C.", "contents": "Correction for cardiac output calculations by the nitrous oxide technique. Calculations of cardiac output using the N2O technique as described by Becklake et. al. (J. Appl. Physiol. 17: 579, 1962) result in spurious values when barometric pressure or body temperature deviates from 760 Torr or 37.0 degrees C, respectively. Nitrous oxide (N2O) uptake (VN2O) must be corrected to standard pressure, dry at 37.0 degrees C.", "PMID": 956109}
{"id": "pubmed23n0032_1978", "title": "A new method of measuring skin humidity.", "content": "A device suitable for the long-time measurement of relative skin humidity is described. This is a simple circuit with a resistance bridge for lithium chloride sensors connected to a digitally steered logic circuit, which causes dried air to stream intermittently through a measuring chamber placed on the skin in such a way that the relative humidity over the skin is maintained at a constant level. The number of switching time periods is proportional to the relative humidity (secretion performance) of the skin and can be counted, recorded, or directly fed into a digital calculator. The apparatus, including a two-channel version with skin temperature recording, has proved useful in sleep investigations under extreme climatic conditions.", "contents": "A new method of measuring skin humidity. A device suitable for the long-time measurement of relative skin humidity is described. This is a simple circuit with a resistance bridge for lithium chloride sensors connected to a digitally steered logic circuit, which causes dried air to stream intermittently through a measuring chamber placed on the skin in such a way that the relative humidity over the skin is maintained at a constant level. The number of switching time periods is proportional to the relative humidity (secretion performance) of the skin and can be counted, recorded, or directly fed into a digital calculator. The apparatus, including a two-channel version with skin temperature recording, has proved useful in sleep investigations under extreme climatic conditions.", "PMID": 956110}
{"id": "pubmed23n0032_1979", "title": "A development of the oxyhemoglobin dissociation curve analyzer.", "content": "The development and improvement of an oxyhemoglobin dissociation curve analyzer is described. PO2 electrode performance was improved by electrochemical means and circuits are described for processing the PO2 and pH signals from the analyzer. A circuit for automatically correcting the curve for Bohr shifts from pH 7.40 is described, and the performance of the Bohr shift unit is verified by experiment. The analyzer produces curves under standard conditions of PCO2 40 mmHg, pH 7.40, and 37 degrees C.", "contents": "A development of the oxyhemoglobin dissociation curve analyzer. The development and improvement of an oxyhemoglobin dissociation curve analyzer is described. PO2 electrode performance was improved by electrochemical means and circuits are described for processing the PO2 and pH signals from the analyzer. A circuit for automatically correcting the curve for Bohr shifts from pH 7.40 is described, and the performance of the Bohr shift unit is verified by experiment. The analyzer produces curves under standard conditions of PCO2 40 mmHg, pH 7.40, and 37 degrees C.", "PMID": 956111}
{"id": "pubmed23n0032_1980", "title": "Method for chronic recording of electroencephalographic activity in conscious dogs.", "content": "The difficulties involved in recording high-quality electroencephalograms chronically from conscious dogs have been resolved by devising an implantable pure silver electrode assembly that can be screwed permanently into the skull. The assembly eliminates electromyographic artifacts and provides high-fidelity tracings. Construction is simple and commercially available, inexpensive parts are used. Highly satisfactory records can be obtained over periods of months.", "contents": "Method for chronic recording of electroencephalographic activity in conscious dogs. The difficulties involved in recording high-quality electroencephalograms chronically from conscious dogs have been resolved by devising an implantable pure silver electrode assembly that can be screwed permanently into the skull. The assembly eliminates electromyographic artifacts and provides high-fidelity tracings. Construction is simple and commercially available, inexpensive parts are used. Highly satisfactory records can be obtained over periods of months.", "PMID": 956112}
{"id": "pubmed23n0032_1981", "title": "Identification of several unique, low-molecular-weight basic proteins in dormant spores of clastridium bifermentans and their degradation during spore germination.", "content": "Two acid-soluble, low-molecular-weight basic proteins comprise approximately 20% of the protein in dormant spores of Clostridium bifermentans. Both of these proteins are rapidly degraded during spore germination.", "contents": "Identification of several unique, low-molecular-weight basic proteins in dormant spores of clastridium bifermentans and their degradation during spore germination. Two acid-soluble, low-molecular-weight basic proteins comprise approximately 20% of the protein in dormant spores of Clostridium bifermentans. Both of these proteins are rapidly degraded during spore germination.", "PMID": 956113}
{"id": "pubmed23n0032_1982", "title": "Bacterial characteristics determining the potential host range of bacteriophage T4.", "content": "Many members of the Enterobacteriaceae, but not other gram-negative organisms, apparently carry a specific recognition site for the T4 tail tube on their cytoplasmic membranes.", "contents": "Bacterial characteristics determining the potential host range of bacteriophage T4. Many members of the Enterobacteriaceae, but not other gram-negative organisms, apparently carry a specific recognition site for the T4 tail tube on their cytoplasmic membranes.", "PMID": 956114}
{"id": "pubmed23n0032_1983", "title": "Cellular localization of uridine 5'-diphosphate-N-acetyl-D-glucosamine oxidoreductase activity in Citrobacter freundii.", "content": "The uridine 5'-diphosphate-N-acetyl-d-glucosamine oxidoreductase activity of Citrobacter freundii ATCC 10053 was found to be located in the soluble cytoplasmic fraction of lysed spheroplasts.", "contents": "Cellular localization of uridine 5'-diphosphate-N-acetyl-D-glucosamine oxidoreductase activity in Citrobacter freundii. The uridine 5'-diphosphate-N-acetyl-d-glucosamine oxidoreductase activity of Citrobacter freundii ATCC 10053 was found to be located in the soluble cytoplasmic fraction of lysed spheroplasts.", "PMID": 956115}
{"id": "pubmed23n0032_1984", "title": "Hydrogenase and ribulose diphosphate carboxylase during autotrophic, heterotrophic, and mixotrophic growth of scotochromogenic mycobacteria.", "content": "Two key autotrophic enzyme systems, hydrogenase and ribulose diphosphate carboxylase, were examined in Mycobacterium gordonae and two other chemolithotrophic, scotochromogenic mycobacteria under different cultural conditions. In all three organisms both enzymes were inducible and were produced in significant levels only in the presence of the specific substrate, hydrogen or carbon dioxide. M. gordonae exhibited increased growth rates and yields, indicating mixotrophic growth, in the presence of a number of single organic substrates, including acetate, pyruvate, glucose, fructose, and glycerol. In contrast to other aerobic hydrogen autotrophs, the presence of either acetate or pyruvate did not repress ribulose diphosphate carboxylase, and mixotrophic growth was rapid with these substrates. In the absence of carbon dioxide, growth in glycerol medium under an atmosphere of hydrogen and oxygen was severely inhibited, even with cells preadapted to heterotrophic growth on glycerol. Cyclic adenosine monophosphate was not effective in inducing hydrogenase or carboxylase in heterotrophic, mixotrophic, or hydrogen-inhibited cultures.", "contents": "Hydrogenase and ribulose diphosphate carboxylase during autotrophic, heterotrophic, and mixotrophic growth of scotochromogenic mycobacteria. Two key autotrophic enzyme systems, hydrogenase and ribulose diphosphate carboxylase, were examined in Mycobacterium gordonae and two other chemolithotrophic, scotochromogenic mycobacteria under different cultural conditions. In all three organisms both enzymes were inducible and were produced in significant levels only in the presence of the specific substrate, hydrogen or carbon dioxide. M. gordonae exhibited increased growth rates and yields, indicating mixotrophic growth, in the presence of a number of single organic substrates, including acetate, pyruvate, glucose, fructose, and glycerol. In contrast to other aerobic hydrogen autotrophs, the presence of either acetate or pyruvate did not repress ribulose diphosphate carboxylase, and mixotrophic growth was rapid with these substrates. In the absence of carbon dioxide, growth in glycerol medium under an atmosphere of hydrogen and oxygen was severely inhibited, even with cells preadapted to heterotrophic growth on glycerol. Cyclic adenosine monophosphate was not effective in inducing hydrogenase or carboxylase in heterotrophic, mixotrophic, or hydrogen-inhibited cultures.", "PMID": 956116}
{"id": "pubmed23n0032_1985", "title": "Phosphorylation of glycerol and dihydroxyacetone in Acetobacter xylinum and its possible regulatory role.", "content": "Extracts of Acetobacter xylinum catalyze the phosphorylation of glycerol and dihydroxyacetone (DHA) by adenosine 5'-triphosphate (ATP) to form, respectively, L-alpha-glycerophosphate and DHA phosphate. The ability to promote phosphorylation of glycerol and DHA was higher in glycerol-grown cells than in glucose- or succinate-grown cells. The activity of glycerol kinase in extracts is compatible with the overall rate of glycerol oxidation in vivo. The glycerol-DHA kinase has been purified 210-fold from extracts, and its molecular weight was determined to be 50,000 by gel filtration. The glycerol kinase to DHA kinase activity ratio remained essentially constant at 1.6 at all stages of purification. The optimal pH for both reactions was 8.4 to 9.2. Reaction rates with the purified enzyme were hyperbolic functions of glycerol, DHA, and ATP. The Km for glycerol is 0.5 mM and that for DHA is 5 mM; both are independent of the ATP concentration. The Km for ATP in both kinase reactions is 0.5 mM and is independent of glycerol and DHA concentrations. Glycerol and DHA are competitive substrates with Ki values equal to their respective Km values as substrates. D-Glyceraldehyde and l-Glyceraldehyde were not phosphorylated and did not inhibit the enzyme. Among the nucleotide triphosphates tested, only ATP was active as the phosphoryl group donor. Fructose diphosphate (FDP) inhibited both kinase activities competitively with respect to ATP (Ki= 0.02 mM) and noncompetitively with respect to glycerol and DHA. Adenosine 5'-diphosphate (ADP) and adenosine 5'-monophosphate (AMP) inhibited both enzymic activities competitively with respect to ATP (Ki (ADP) = 0.4 mM; Ki (AMP) =0.25 mM). A. xylinum cells with a high FDP content did not grow on glycerol. Depletion of cellular FDP by starvation enabled rapid growth on glycerol. It is concluded that a single enzyme from A. xylinum is responsible for the phosphorylation of both glycerol and DHA. This as well as the sensitivity of the enzyme to inhibition by FDP and AMP suggest that it has a regulatory role in glycerol metabolism.", "contents": "Phosphorylation of glycerol and dihydroxyacetone in Acetobacter xylinum and its possible regulatory role. Extracts of Acetobacter xylinum catalyze the phosphorylation of glycerol and dihydroxyacetone (DHA) by adenosine 5'-triphosphate (ATP) to form, respectively, L-alpha-glycerophosphate and DHA phosphate. The ability to promote phosphorylation of glycerol and DHA was higher in glycerol-grown cells than in glucose- or succinate-grown cells. The activity of glycerol kinase in extracts is compatible with the overall rate of glycerol oxidation in vivo. The glycerol-DHA kinase has been purified 210-fold from extracts, and its molecular weight was determined to be 50,000 by gel filtration. The glycerol kinase to DHA kinase activity ratio remained essentially constant at 1.6 at all stages of purification. The optimal pH for both reactions was 8.4 to 9.2. Reaction rates with the purified enzyme were hyperbolic functions of glycerol, DHA, and ATP. The Km for glycerol is 0.5 mM and that for DHA is 5 mM; both are independent of the ATP concentration. The Km for ATP in both kinase reactions is 0.5 mM and is independent of glycerol and DHA concentrations. Glycerol and DHA are competitive substrates with Ki values equal to their respective Km values as substrates. D-Glyceraldehyde and l-Glyceraldehyde were not phosphorylated and did not inhibit the enzyme. Among the nucleotide triphosphates tested, only ATP was active as the phosphoryl group donor. Fructose diphosphate (FDP) inhibited both kinase activities competitively with respect to ATP (Ki= 0.02 mM) and noncompetitively with respect to glycerol and DHA. Adenosine 5'-diphosphate (ADP) and adenosine 5'-monophosphate (AMP) inhibited both enzymic activities competitively with respect to ATP (Ki (ADP) = 0.4 mM; Ki (AMP) =0.25 mM). A. xylinum cells with a high FDP content did not grow on glycerol. Depletion of cellular FDP by starvation enabled rapid growth on glycerol. It is concluded that a single enzyme from A. xylinum is responsible for the phosphorylation of both glycerol and DHA. This as well as the sensitivity of the enzyme to inhibition by FDP and AMP suggest that it has a regulatory role in glycerol metabolism.", "PMID": 956117}
{"id": "pubmed23n0032_1986", "title": "Regulation of molybdate transport by Clostridium pasteurianum.", "content": "The regulation of the molybdate (MoO42-) transport activity of Clostridium pasteurianum has been studied by observing the effects of NH3, carbamyl phosphate, MoO42-, and chloramphenicol on the ability of cells to take up MoO42-. Compared with cells fixing N2, cells grown in the presence of 1 mM NH3 are greater than 95% repressed for MoO42- transport. Uptake activity begins to increase just before NH exhaustion (under Ar or N2) and continues to increase throughout the lag period as cells shift from NH3-growing to N2-fixing conditions. When cells are shifted from N2-fixing to NH3-growing conditions the transport activity per fixed number of cells decreases by increase of bells in absence of transport synthesis. Carbamyl phosphate (greater than or equal to 15 mM) but not NH3 inhibits 58% of the in vitro uptake activity. When 1 mM carbamyl phosphate is added just before the exhaustion of NH3, the transport activity, measured 2 h later, is 100% repressed. Cells grown in the presence of high MoO42- (1mM) are 80% repressed for MoO42- transport. Synthesis of the MoO42- transport system is also completely stopped when chloramphenicol (300 mug/ml) is added just before the exhaustion oNH 3 from the medium. These findings demonstrate that the ability of cells to transport MoO42- is dependent upon new protein synthesis and can be repressed by high levels of substrate. The regulation of MoO42- uptake by NH3 or carbamyl phosphate closely parallels the regulation of nitrogenase activity. Activity of neither nitrogenase component (Fe protein or MoFe protein) was detected even 3 h after the exhaustion of the NH3 if either MoO42- was absent or if WO42- was present in place of MoO42-. The duration of the diauxic lag increases with decreasing concentration of MoO42- in the medium. If no MoO42- is present the lag continues indefinitely. If MoO42- is added late in the lag period, growth under N2-fixing conditions resumes but only after a normal induction period.", "contents": "Regulation of molybdate transport by Clostridium pasteurianum. The regulation of the molybdate (MoO42-) transport activity of Clostridium pasteurianum has been studied by observing the effects of NH3, carbamyl phosphate, MoO42-, and chloramphenicol on the ability of cells to take up MoO42-. Compared with cells fixing N2, cells grown in the presence of 1 mM NH3 are greater than 95% repressed for MoO42- transport. Uptake activity begins to increase just before NH exhaustion (under Ar or N2) and continues to increase throughout the lag period as cells shift from NH3-growing to N2-fixing conditions. When cells are shifted from N2-fixing to NH3-growing conditions the transport activity per fixed number of cells decreases by increase of bells in absence of transport synthesis. Carbamyl phosphate (greater than or equal to 15 mM) but not NH3 inhibits 58% of the in vitro uptake activity. When 1 mM carbamyl phosphate is added just before the exhaustion of NH3, the transport activity, measured 2 h later, is 100% repressed. Cells grown in the presence of high MoO42- (1mM) are 80% repressed for MoO42- transport. Synthesis of the MoO42- transport system is also completely stopped when chloramphenicol (300 mug/ml) is added just before the exhaustion oNH 3 from the medium. These findings demonstrate that the ability of cells to transport MoO42- is dependent upon new protein synthesis and can be repressed by high levels of substrate. The regulation of MoO42- uptake by NH3 or carbamyl phosphate closely parallels the regulation of nitrogenase activity. Activity of neither nitrogenase component (Fe protein or MoFe protein) was detected even 3 h after the exhaustion of the NH3 if either MoO42- was absent or if WO42- was present in place of MoO42-. The duration of the diauxic lag increases with decreasing concentration of MoO42- in the medium. If no MoO42- is present the lag continues indefinitely. If MoO42- is added late in the lag period, growth under N2-fixing conditions resumes but only after a normal induction period.", "PMID": 956118}
{"id": "pubmed23n0032_1987", "title": "Characterization of an Acholeplasma laidlawii variant with a REP- phenotype.", "content": "An Acholeplasma laidlawii variant has been isolated that has a REP- phenotype. The properties of this variant, relative to parental cells, are: (i) it exhibits no change in cell growth kinetics; (ii) it does not propagate single-stranded deoxyribonucleic acid (DNA) mycoplasmaviruses but does propagate double-stranded DNA mycoplasmaviruses; (iii) it converts parental circular single-stranded mycoplasmavirus DNA to double-stranded replicative forms that are not replicated further; (iv) it exhibits no change in host modification and restriction; and (v) it has an increased ultraviolet light sensitivity. The REP- isolate is the first stable mycoplasma variant to which a physiological defect has been attributed.", "contents": "Characterization of an Acholeplasma laidlawii variant with a REP- phenotype. An Acholeplasma laidlawii variant has been isolated that has a REP- phenotype. The properties of this variant, relative to parental cells, are: (i) it exhibits no change in cell growth kinetics; (ii) it does not propagate single-stranded deoxyribonucleic acid (DNA) mycoplasmaviruses but does propagate double-stranded DNA mycoplasmaviruses; (iii) it converts parental circular single-stranded mycoplasmavirus DNA to double-stranded replicative forms that are not replicated further; (iv) it exhibits no change in host modification and restriction; and (v) it has an increased ultraviolet light sensitivity. The REP- isolate is the first stable mycoplasma variant to which a physiological defect has been attributed.", "PMID": 956119}
{"id": "pubmed23n0032_1988", "title": "Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842.", "content": "pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, although at a greatly reduced frequency. pGC1 has a molecular weight of about 33.0 x 10(6), has a base composition of about 44 mol% guanine plus cytosine, and is fi-.", "contents": "Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842. pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, although at a greatly reduced frequency. pGC1 has a molecular weight of about 33.0 x 10(6), has a base composition of about 44 mol% guanine plus cytosine, and is fi-.", "PMID": 956120}
{"id": "pubmed23n0032_1989", "title": "Catechol oxygenases of Pseudomonas putida mutant strains.", "content": "Investigation of a mutant strain of Pseudomonas putida NCIB 10015, strain PsU-E1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. Additional mutants of both wild-type and mutant strains PsU-E1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. These findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate inducers for the one catechol 1,2-oxygenase enzyme. Comparisons of the physical properties of the catechol 1,2-oxygenases formed in response to the two different inducers show no significant differences, so it is more probable that the two proteins are the product of the same gene. Sufficient enzymes of the ortho-fission pathway are induced in the wild-type strain by the initial substrate benzoate (or an early intermediate) to commit that substrate to metabolism by ortho fission exclusively. A mechanism exists that permits metabolism of catechol by meta fission if the ortho-fission enzymes are unable to prevent its intracellular accumulation.", "contents": "Catechol oxygenases of Pseudomonas putida mutant strains. Investigation of a mutant strain of Pseudomonas putida NCIB 10015, strain PsU-E1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. Additional mutants of both wild-type and mutant strains PsU-E1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. These findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate inducers for the one catechol 1,2-oxygenase enzyme. Comparisons of the physical properties of the catechol 1,2-oxygenases formed in response to the two different inducers show no significant differences, so it is more probable that the two proteins are the product of the same gene. Sufficient enzymes of the ortho-fission pathway are induced in the wild-type strain by the initial substrate benzoate (or an early intermediate) to commit that substrate to metabolism by ortho fission exclusively. A mechanism exists that permits metabolism of catechol by meta fission if the ortho-fission enzymes are unable to prevent its intracellular accumulation.", "PMID": 956121}
{"id": "pubmed23n0032_1990", "title": "Involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by Pseudomonas convexa.", "content": "A microorganism capable of degrading DL-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as Pseudomonas convexa. It was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. All the enzymes of the pathway were demonstrated in cell-free extracts. L-Mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicotinamide adenine dinucleotide phosphate, reduced form, and Fe2+ for its activity. The next enzyme, L-4-hydroxymandelate oxidase (decarboxylating), a particulate enzyme, requires flavine adenine dinucleotide and Mn2+ for its activity. A nicotinamide adenine dinucleotide-dependent, as well as a nicotinamide adenine dinucleotide phosphate-dependent, benzaldehyde dehydrogenase has been resolved and partially purified.", "contents": "Involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by Pseudomonas convexa. A microorganism capable of degrading DL-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as Pseudomonas convexa. It was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. All the enzymes of the pathway were demonstrated in cell-free extracts. L-Mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicotinamide adenine dinucleotide phosphate, reduced form, and Fe2+ for its activity. The next enzyme, L-4-hydroxymandelate oxidase (decarboxylating), a particulate enzyme, requires flavine adenine dinucleotide and Mn2+ for its activity. A nicotinamide adenine dinucleotide-dependent, as well as a nicotinamide adenine dinucleotide phosphate-dependent, benzaldehyde dehydrogenase has been resolved and partially purified.", "PMID": 956122}
{"id": "pubmed23n0032_1991", "title": "Genetic linkage of chromosomal tetracycline resistance and pigmentation to a purine auxotrophic marker and the isoleucine-valine-leucine structural genes in Staphylococcus aureus.", "content": "Three tetracycline resistance determinants (tmn-3106, tmn-3110, and tmn-3511) reported by Asheshov (1975) to be chromosomal in Staphylococcus aureus have been linked by transformation to a purine auxotrophic marker (pur-110), a cluster of eight genes involved in the biosynthesis of isoleucine, valine, and leucine (the ilv-leu region), a marker (ilvR10) that may be involved in the regulation of the ilv-leu region, and a gene involved in pigmentation (pig-131). The linkage group thus defined is tmn-3106-pur-110-ilvR10-(ilv-leu)-pig-131. The orientation of the ilv-leu region relative to ilvR10 and pig-131 was not determined. The tmn-3106, tmn-3110, and tmn-3511 determinants exhibit the same linkage relationships to the other markers. It is concluded that this linkage group represents a portion of the chromosome of S. aureus.", "contents": "Genetic linkage of chromosomal tetracycline resistance and pigmentation to a purine auxotrophic marker and the isoleucine-valine-leucine structural genes in Staphylococcus aureus. Three tetracycline resistance determinants (tmn-3106, tmn-3110, and tmn-3511) reported by Asheshov (1975) to be chromosomal in Staphylococcus aureus have been linked by transformation to a purine auxotrophic marker (pur-110), a cluster of eight genes involved in the biosynthesis of isoleucine, valine, and leucine (the ilv-leu region), a marker (ilvR10) that may be involved in the regulation of the ilv-leu region, and a gene involved in pigmentation (pig-131). The linkage group thus defined is tmn-3106-pur-110-ilvR10-(ilv-leu)-pig-131. The orientation of the ilv-leu region relative to ilvR10 and pig-131 was not determined. The tmn-3106, tmn-3110, and tmn-3511 determinants exhibit the same linkage relationships to the other markers. It is concluded that this linkage group represents a portion of the chromosome of S. aureus.", "PMID": 956123}
{"id": "pubmed23n0032_1992", "title": "Plasmid-protein relaxation complexes in Staphylococcus aureus.", "content": "Protein-deoxyribonucleic acid relaxation complexes have been demonstrated for six Staphylococcus aureus plasmids out of sixteen examined. Four of these encode stretomycin resistence, have molecular weights of about 2.7 x 10(6), and are isolated as supercoiled molecules that are virtally 100% relaxable by treatment with sodium dodecyl sulfate. It is probable that these four isolates represent a single widely disseminated plasmid species. The other two plasmids showing relaxation complexes have molecular weights of about 3 x 10(6) and encode chloramphenicol resistance. The complexes in these cases are unstable, and it has not been possible to induce more than 50% relaxation by any of the standard treatments. Ten other plasmids do not show detectable complexes. These include three penicillinase plasmids, four tetracycline-resistance plasmids, one plasmid carrying kanamycin-neomycin resistance, and finally, two chloramphenicol-resistance plasmids.", "contents": "Plasmid-protein relaxation complexes in Staphylococcus aureus. Protein-deoxyribonucleic acid relaxation complexes have been demonstrated for six Staphylococcus aureus plasmids out of sixteen examined. Four of these encode stretomycin resistence, have molecular weights of about 2.7 x 10(6), and are isolated as supercoiled molecules that are virtally 100% relaxable by treatment with sodium dodecyl sulfate. It is probable that these four isolates represent a single widely disseminated plasmid species. The other two plasmids showing relaxation complexes have molecular weights of about 3 x 10(6) and encode chloramphenicol resistance. The complexes in these cases are unstable, and it has not been possible to induce more than 50% relaxation by any of the standard treatments. Ten other plasmids do not show detectable complexes. These include three penicillinase plasmids, four tetracycline-resistance plasmids, one plasmid carrying kanamycin-neomycin resistance, and finally, two chloramphenicol-resistance plasmids.", "PMID": 956124}
{"id": "pubmed23n0032_1993", "title": "XYL, a nonconjugative xylene-degradative plasmid in Pseudomonas Pxy.", "content": "Pseudomanas Pxy metabolizes p- or m-xylene through intermediate formation of the corresponding methylbenzyl alcohol and toluic acid via the meta pathway. The strain Pseudomonas Pxy spontaneously loses its ability to grow with xylene or toluate, and the rate of loss of this ability is greatly enhanced by treatment of the cells with mitomycin C. The assay of enzymes involved in xylene degradation in xylene-negative Pxy cells indicates the loss of the entire enzyme complement of the pathway. The genes specifying all the xylene-degradative enzymes, including those of the meta pathway, appear to be borne on a nonconjugative plasmid and can be transferred to xylene-negative Pxy or P. putida strain PpG1 cells only in the presence of a transfer plasmid termed factor K. When transferred to strain PpG1, the xylene-degradative plasmid, termed XYL, coexists stably with factor K, but transduction of XYL is not accompanied by a cotransfer of factor K. XYL appears to be compatible wit- all the other known degradative plasmids in P. putida. The xylene pathway is inducible in wild-type Pxy as well as in Pxy and PpG1 exconjugants, suggesting the cotransfer of regulatory genes along with the plasmid. The enzymes converting xylene to toluate are induced by xylene, methylbenzyl alcohol, or the aldehyde derivatives but not significantly by toluate, whereas catechol dioxygenase and other enzymes are induced by toluates and presumable by xylene as well.", "contents": "XYL, a nonconjugative xylene-degradative plasmid in Pseudomonas Pxy. Pseudomanas Pxy metabolizes p- or m-xylene through intermediate formation of the corresponding methylbenzyl alcohol and toluic acid via the meta pathway. The strain Pseudomonas Pxy spontaneously loses its ability to grow with xylene or toluate, and the rate of loss of this ability is greatly enhanced by treatment of the cells with mitomycin C. The assay of enzymes involved in xylene degradation in xylene-negative Pxy cells indicates the loss of the entire enzyme complement of the pathway. The genes specifying all the xylene-degradative enzymes, including those of the meta pathway, appear to be borne on a nonconjugative plasmid and can be transferred to xylene-negative Pxy or P. putida strain PpG1 cells only in the presence of a transfer plasmid termed factor K. When transferred to strain PpG1, the xylene-degradative plasmid, termed XYL, coexists stably with factor K, but transduction of XYL is not accompanied by a cotransfer of factor K. XYL appears to be compatible wit- all the other known degradative plasmids in P. putida. The xylene pathway is inducible in wild-type Pxy as well as in Pxy and PpG1 exconjugants, suggesting the cotransfer of regulatory genes along with the plasmid. The enzymes converting xylene to toluate are induced by xylene, methylbenzyl alcohol, or the aldehyde derivatives but not significantly by toluate, whereas catechol dioxygenase and other enzymes are induced by toluates and presumable by xylene as well.", "PMID": 956125}
{"id": "pubmed23n0032_1994", "title": "Ultrastructure and elemental composition of dormant and germinating Diplodia maydis spores.", "content": "The ultrastructure of Diplodia maydis spores was studied in thin sections with a transmission electron microscope. Storage vacuoles were evenly distributed in the two cells. Some of the vacuoles that contained a dense osmiophilic sphere(s) were surrounded by a membrane, and had membranous aggregates around their periphery. The sport wall was composed of an electron-dense layer and an electron-translucent layer. An inner cytoplasmic membrane was present. Dormant and germinating spores were studied with scanning electron microscopy and also with a Si (Li) energy-dispersive X-ray analyzer. The dormant spore was ovate and usually two-celled with a central septum. Germination proceeded via a germ tube from the side of one end of the cell. Of several methods for preparation of specimens for X-ray analysis studied, freeze-dried spores mounted on carbon stubs and then further carbon coated gave the best results. X-ray analyses revealed that spore populations contained large amounts of Si, P, Cl, and K, smaller amounts of S and Ca, and trace amounts of Mg and Al. Analyses of single spores revealed high K and Cl and low P and Mg at one end of the cell with concomitant low K and Cl and high P and Mg in the central portion and other end of the cell. In two-celled germinating spores, high K and Cl occurred in the end of the nongerminating spore cell, whereas the germinating cell contained high P and Mg and low K and Cl. X-ray image maps revealed that K and Cl were located together at one end of the spore.", "contents": "Ultrastructure and elemental composition of dormant and germinating Diplodia maydis spores. The ultrastructure of Diplodia maydis spores was studied in thin sections with a transmission electron microscope. Storage vacuoles were evenly distributed in the two cells. Some of the vacuoles that contained a dense osmiophilic sphere(s) were surrounded by a membrane, and had membranous aggregates around their periphery. The sport wall was composed of an electron-dense layer and an electron-translucent layer. An inner cytoplasmic membrane was present. Dormant and germinating spores were studied with scanning electron microscopy and also with a Si (Li) energy-dispersive X-ray analyzer. The dormant spore was ovate and usually two-celled with a central septum. Germination proceeded via a germ tube from the side of one end of the cell. Of several methods for preparation of specimens for X-ray analysis studied, freeze-dried spores mounted on carbon stubs and then further carbon coated gave the best results. X-ray analyses revealed that spore populations contained large amounts of Si, P, Cl, and K, smaller amounts of S and Ca, and trace amounts of Mg and Al. Analyses of single spores revealed high K and Cl and low P and Mg at one end of the cell with concomitant low K and Cl and high P and Mg in the central portion and other end of the cell. In two-celled germinating spores, high K and Cl occurred in the end of the nongerminating spore cell, whereas the germinating cell contained high P and Mg and low K and Cl. X-ray image maps revealed that K and Cl were located together at one end of the spore.", "PMID": 956127}
{"id": "pubmed23n0032_1995", "title": "Energy coupling in the active transport of proline and glutamate by the photosynthetic halophile Ectothiorhodospira halophila.", "content": "When illuminated, washed cell suspensions of Ectothiorhodospira halophila carry out a concentrative uptake of glutamate or proline. Dark-exposed cells accumulate glutamate but not proline. Proline transport was strongly inhibited by carbonylcyanide-m-chlorophenylhydrazone (CCCP), a proton permeant that uncouples photophosphorylation, and by 2-heptyl-4-hydroxyquinoline-n-oxide (HQNO), an inhibitor of photosynthetic electron transport. A stimulation of proline uptake was effected by N,N'-dicyclohexylcarbodiimide (DCCD), an inhibitor of membrane adenosine triphosphatase (ATPase) which catalyzes the phosphorylation. These findings suggest that the driving force for proline transport is the proton-motive force established during photosynthetic electron transport. Glutamate uptake in the light was inhibited by CCCP and HQNO, but to a lesser extent than was the proline system. DCCD caused a mild inhibition of glutamate uptake in the light, but strongly inhibited the uptake by dark-exposed cells. CCCP strongly inhibited glutamate uptake in the dark. The light-dependent transport of glutamate is apparently driven by the proton-motive force established during photosynthetic electron transport. Hydrolysis of adenosine triphosphate (ATP) by membrane ATPase apparently establishes the proton-motive force to drive the light-independent transport. These conclusions were supported by demonstrating that light- or dark-exposed cells accumulate [3H]triphenylmethylphosphonium, a lipid-soluble cation. Several lines of indirect evidence indicated that the proline system required higher levels of energy than did the glutamate system(s). This could explain why ATP hydrolysis does not drive proline transport in the dark. Membrane vesicles were prepared by the sonic treatment of E. halophila spheroplasts. The vesicles contained active systems for the uptake of proline and glutamate.", "contents": "Energy coupling in the active transport of proline and glutamate by the photosynthetic halophile Ectothiorhodospira halophila. When illuminated, washed cell suspensions of Ectothiorhodospira halophila carry out a concentrative uptake of glutamate or proline. Dark-exposed cells accumulate glutamate but not proline. Proline transport was strongly inhibited by carbonylcyanide-m-chlorophenylhydrazone (CCCP), a proton permeant that uncouples photophosphorylation, and by 2-heptyl-4-hydroxyquinoline-n-oxide (HQNO), an inhibitor of photosynthetic electron transport. A stimulation of proline uptake was effected by N,N'-dicyclohexylcarbodiimide (DCCD), an inhibitor of membrane adenosine triphosphatase (ATPase) which catalyzes the phosphorylation. These findings suggest that the driving force for proline transport is the proton-motive force established during photosynthetic electron transport. Glutamate uptake in the light was inhibited by CCCP and HQNO, but to a lesser extent than was the proline system. DCCD caused a mild inhibition of glutamate uptake in the light, but strongly inhibited the uptake by dark-exposed cells. CCCP strongly inhibited glutamate uptake in the dark. The light-dependent transport of glutamate is apparently driven by the proton-motive force established during photosynthetic electron transport. Hydrolysis of adenosine triphosphate (ATP) by membrane ATPase apparently establishes the proton-motive force to drive the light-independent transport. These conclusions were supported by demonstrating that light- or dark-exposed cells accumulate [3H]triphenylmethylphosphonium, a lipid-soluble cation. Several lines of indirect evidence indicated that the proline system required higher levels of energy than did the glutamate system(s). This could explain why ATP hydrolysis does not drive proline transport in the dark. Membrane vesicles were prepared by the sonic treatment of E. halophila spheroplasts. The vesicles contained active systems for the uptake of proline and glutamate.", "PMID": 956126}
{"id": "pubmed23n0032_1996", "title": "Motility as a morphogenic character in the genus Arthrobacter.", "content": "Motility in Arthrobacter atrocyaneus, A. citreus, and A. simplex was found to correlate with the morphogenic cycle of these organisms. The percentage of the A. atrocyaneus and A. simplex populations that were flagellated at a given time during the growth cycle differed significantly from that of the normorphogenic Pseudomonas aeruginosa population. Flagellation in A. atrocyaneus was shown to be dependent upon the morphogenic cycle rather than upon growth. The commitment to flagellar synthesis in A. atrocyaneus was found to occur only after induction to the rod morphology. Flagellar synthesis in A. atrocyaneus was shown to be restricted to only a small segment of the morphogenic cycle.", "contents": "Motility as a morphogenic character in the genus Arthrobacter. Motility in Arthrobacter atrocyaneus, A. citreus, and A. simplex was found to correlate with the morphogenic cycle of these organisms. The percentage of the A. atrocyaneus and A. simplex populations that were flagellated at a given time during the growth cycle differed significantly from that of the normorphogenic Pseudomonas aeruginosa population. Flagellation in A. atrocyaneus was shown to be dependent upon the morphogenic cycle rather than upon growth. The commitment to flagellar synthesis in A. atrocyaneus was found to occur only after induction to the rod morphology. Flagellar synthesis in A. atrocyaneus was shown to be restricted to only a small segment of the morphogenic cycle.", "PMID": 956128}
{"id": "pubmed23n0032_1997", "title": "Isolation and characterization of the rodlet layer of Trichophyton mentagrophytes microconidial wall.", "content": "The rodlet layer of the microconidial wall of Trichophyton mentagrophytes was isolated and partially characterized. The purified microconidial walls were first extracted with urea (8M), mercaptoethanol (1%), and sodium dodecyl sulfate (1%) followed by enzymatic digestion with glusulase (snail intestinal enzymes) and purified (1 leads to 3)-beta-D-glucanase and chitinase. The purified rodlet layer was 15 to 30 nm thick and accounted for approximately 10% of the original wall weight. The pattern of rodlet patches, as revealed by electron microscopy of freeze-etched preparations of the isolated layer, was essentially the same as that observed on the intact microconidial wall. The rodlet layer was found to be resistant to most of the common organic solvents, cell wall lytic enzymes, mild acid treatments, and surface-active agents, but was solubilized in boiling 1 N NaOH with concomitant disorientation of the rodlet patterns. A melanin or melanin-like pigment appeared to be intimately associated with this rodlet layer and was solubilized during a hot-alkali treatment. Protein (80 to 85%) and glucomannan (7 to 10%) were the major components of the rodlet layer. The rodlet layer did not contain any appreciable amounts of lipid or phosphorus.", "contents": "Isolation and characterization of the rodlet layer of Trichophyton mentagrophytes microconidial wall. The rodlet layer of the microconidial wall of Trichophyton mentagrophytes was isolated and partially characterized. The purified microconidial walls were first extracted with urea (8M), mercaptoethanol (1%), and sodium dodecyl sulfate (1%) followed by enzymatic digestion with glusulase (snail intestinal enzymes) and purified (1 leads to 3)-beta-D-glucanase and chitinase. The purified rodlet layer was 15 to 30 nm thick and accounted for approximately 10% of the original wall weight. The pattern of rodlet patches, as revealed by electron microscopy of freeze-etched preparations of the isolated layer, was essentially the same as that observed on the intact microconidial wall. The rodlet layer was found to be resistant to most of the common organic solvents, cell wall lytic enzymes, mild acid treatments, and surface-active agents, but was solubilized in boiling 1 N NaOH with concomitant disorientation of the rodlet patterns. A melanin or melanin-like pigment appeared to be intimately associated with this rodlet layer and was solubilized during a hot-alkali treatment. Protein (80 to 85%) and glucomannan (7 to 10%) were the major components of the rodlet layer. The rodlet layer did not contain any appreciable amounts of lipid or phosphorus.", "PMID": 956129}
{"id": "pubmed23n0032_1998", "title": "Electron microscope studies of conditional spore cortexless mutants of Bacillus sphaericus.", "content": "Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,epsilon-diaminopimelic acid.", "contents": "Electron microscope studies of conditional spore cortexless mutants of Bacillus sphaericus. Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,epsilon-diaminopimelic acid.", "PMID": 956130}
{"id": "pubmed23n0032_1999", "title": "Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F.", "content": "The replication region fragments from Flac(+) cloned in plasmids pSC138 and pML31 are identical with each other and with EcoRI fragment 5 of plasmid F.", "contents": "Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F. The replication region fragments from Flac(+) cloned in plasmids pSC138 and pML31 are identical with each other and with EcoRI fragment 5 of plasmid F.", "PMID": 956131}
{"id": "pubmed23n0032_2000", "title": "Influence of secretory immunoglobulin A and purified secretory component on dextran-sucrase activity of Streptococcus mutans.", "content": "The net stimulation of dextransucrase EC 2.4.1.5) activity from Streptococcus mutans HS6 by dextran, secretory immunoglobulin A, or secretory component was investigated. Approximately equal stimulation resulted from treatment with these three components.", "contents": "Influence of secretory immunoglobulin A and purified secretory component on dextran-sucrase activity of Streptococcus mutans. The net stimulation of dextransucrase EC 2.4.1.5) activity from Streptococcus mutans HS6 by dextran, secretory immunoglobulin A, or secretory component was investigated. Approximately equal stimulation resulted from treatment with these three components.", "PMID": 956132}
{"id": "pubmed23n0032_2001", "title": "Studies on the subsite structure of amylases. III. Inhibition by gluconolactone of the hydrolysis of maltodextrin catalyzed by glucoamylase from Rhizopus niveus.", "content": "Inhibition by gluconic acid-1 : 5-lactone (gluconolactone) and phenyl alpha-glucoside of the hydrolysis of maltodextrin catalyzed by glucoamylase [EC 3.2.1.3] from Rhizopus niveus was investigated in relation to the subsite structure of the enzyme. Inhibition by gluconolactone was of the mixed type, whereas that by phenyl alpha-glucoside was purely competitive. These inhibition types were consistent with a theoretical prediction based on the assumption that gluconolactone and phenyl alpha-glucoside bind mainly to Subsites 1 and 2, respectively. The inhibitor constant of gluconolatone was determined to be 1.5 mM, which is in good agreement with the dissociation constant estimated by difference spectrophotometry (1.5 mM) (Ohnishi, M, et al. (1975) J. Biochem, 77, 695-703).", "contents": "Studies on the subsite structure of amylases. III. Inhibition by gluconolactone of the hydrolysis of maltodextrin catalyzed by glucoamylase from Rhizopus niveus. Inhibition by gluconic acid-1 : 5-lactone (gluconolactone) and phenyl alpha-glucoside of the hydrolysis of maltodextrin catalyzed by glucoamylase [EC 3.2.1.3] from Rhizopus niveus was investigated in relation to the subsite structure of the enzyme. Inhibition by gluconolactone was of the mixed type, whereas that by phenyl alpha-glucoside was purely competitive. These inhibition types were consistent with a theoretical prediction based on the assumption that gluconolactone and phenyl alpha-glucoside bind mainly to Subsites 1 and 2, respectively. The inhibitor constant of gluconolatone was determined to be 1.5 mM, which is in good agreement with the dissociation constant estimated by difference spectrophotometry (1.5 mM) (Ohnishi, M, et al. (1975) J. Biochem, 77, 695-703).", "PMID": 956133}
{"id": "pubmed23n0032_2002", "title": "Separation of myosin subfragment-1 into fractions containing g1 chani and g2 chain by Sepharose-adipic acid hydrazide-ATP column chromatography.", "content": "Subfragment-1 prepared by chymotryptic digestion of myosin was applied to a column of Sepharose-adipic acid hydrazide-ATP in 1 mM EDTA, 10 mM Tris-HCL (PH 7.6), and 40 mM KCL. Ninety-nine per cent of subfragment-1 was adsorbed on the column in this medium. Fourty-three per cent of the applied protein was eluted with 6 mM ADP in the above buffer and then 52% was eluted with 1 mM EDTA, 10 mM Tris-HCL (pH 7.6), AND 0.7 M KCL. The former fraction contained g3 chain and the latter g1 chain. These fractions were apparently the same as the components, p2 and p1, respectively, isolated by ion-exchange chromatography using DEAE-cellulose (Yagi & Otani (1974) J. Biochem. 76, 365-373). No significant difference of ADP binding was found between the two fractions, both could bind about 0.5 mole per 10(5) g of protein. The preparation of the two subfragment-1 fractions is described.", "contents": "Separation of myosin subfragment-1 into fractions containing g1 chani and g2 chain by Sepharose-adipic acid hydrazide-ATP column chromatography. Subfragment-1 prepared by chymotryptic digestion of myosin was applied to a column of Sepharose-adipic acid hydrazide-ATP in 1 mM EDTA, 10 mM Tris-HCL (PH 7.6), and 40 mM KCL. Ninety-nine per cent of subfragment-1 was adsorbed on the column in this medium. Fourty-three per cent of the applied protein was eluted with 6 mM ADP in the above buffer and then 52% was eluted with 1 mM EDTA, 10 mM Tris-HCL (pH 7.6), AND 0.7 M KCL. The former fraction contained g3 chain and the latter g1 chain. These fractions were apparently the same as the components, p2 and p1, respectively, isolated by ion-exchange chromatography using DEAE-cellulose (Yagi & Otani (1974) J. Biochem. 76, 365-373). No significant difference of ADP binding was found between the two fractions, both could bind about 0.5 mole per 10(5) g of protein. The preparation of the two subfragment-1 fractions is described.", "PMID": 956134}
{"id": "pubmed23n0032_2003", "title": "Spectral intermediates during the reduction of hepatic microsomal cytochrome P-450.", "content": "The slow reduction of microsomal cytochrome P-450 by dithionite consists of an initial fast and then a slow phase. During reduction of aniline and azide complexes with cytochrome P-450, an intermediate spectrum developed in the fast phase and changed to that of the reduced form in the slow phase. Only the spectra in the slow phase had an isosbestic point. No intermediate spectrum detectable during reduction of the cyanide complex and native-cytochrome P-450. Carbon monoxide accelerated the reaction, causing complete reduction in the initial phase. The electron spin resonance spectrum of cytochrome P450 was greatly reduced in the initial phase of reduction with dithionite. These results indicate that reduction of the aniline and azide complexes of cytochrome P-450 involves two steps: first reduction of cytochrome P-450 and then some changes in reduced state. The aniline and cyanide difference spectra of reduced cytochrome P-450 showed peaks at 423 nm and 429 nm, respectively, while that of azide had a peak at 445 nm and a trough at 404 nm. An essay method to obtain the difference spectrum of reduced minus oxidized cytochrome P-450 using a spectral data processor is reported. The effects of other NADH-nonreducible pigments on the spectrum is eliminated by this procedure, provided these pigments are rapidly reduced by dithionite. Therefore, the spectrum obtained was slightly differed from that measured by the usual method, especially in the region of 425 nm.", "contents": "Spectral intermediates during the reduction of hepatic microsomal cytochrome P-450. The slow reduction of microsomal cytochrome P-450 by dithionite consists of an initial fast and then a slow phase. During reduction of aniline and azide complexes with cytochrome P-450, an intermediate spectrum developed in the fast phase and changed to that of the reduced form in the slow phase. Only the spectra in the slow phase had an isosbestic point. No intermediate spectrum detectable during reduction of the cyanide complex and native-cytochrome P-450. Carbon monoxide accelerated the reaction, causing complete reduction in the initial phase. The electron spin resonance spectrum of cytochrome P450 was greatly reduced in the initial phase of reduction with dithionite. These results indicate that reduction of the aniline and azide complexes of cytochrome P-450 involves two steps: first reduction of cytochrome P-450 and then some changes in reduced state. The aniline and cyanide difference spectra of reduced cytochrome P-450 showed peaks at 423 nm and 429 nm, respectively, while that of azide had a peak at 445 nm and a trough at 404 nm. An essay method to obtain the difference spectrum of reduced minus oxidized cytochrome P-450 using a spectral data processor is reported. The effects of other NADH-nonreducible pigments on the spectrum is eliminated by this procedure, provided these pigments are rapidly reduced by dithionite. Therefore, the spectrum obtained was slightly differed from that measured by the usual method, especially in the region of 425 nm.", "PMID": 956135}
{"id": "pubmed23n0032_2004", "title": "The mechanism of activation of bovine prothrombin by an activator isolated from Echis carinatus venon and characterization of the new active intermediates.", "content": "Bovine prothrombin was activated, in both the absence and presence of dissopropyphosphofluoridate (DEP) and benzamidine, by an activator which was highly purified from the venom of Echis carinatus (saw-scaled viper, ECV). The process of activation was monitored by sodium dodecysulfate (SDS)-polyacrylamide gel electrophoresis, and the reaction products were isolated and chemically characterized. In the absence of the inhibitors, prothrombin yielded two fragments with molecular weights of 28,000 and 57,000, of which the former was the N-terminal fragment of the zymogen and the latter was intermediate 1, consisting of a single polypeptide chain. Intermediate 1 was subsequently converted to an active intermediate, named intermediate ECV, without decrease of molecular weight. This new intermediate ECV, which showed little clotting activity but a strong alpha-N-tosyl-L-arginine methyl ester (TAME)-esterolytic activity and which bound with hirudin or antithrombin III, consisted of two polypeptide chains with molecular weights of 35,000 of 27,000 daltons. The former was indentified as the thrombin B chain with the N-terminal sequence Ile-Val-Glu-Gly and C-terminal serine, and the latter was a fragment with N-terminal Ser-Gly-Gly, linked to the thrombin A chain. On prolonged incubation, intermediate ECV autocaralytically yielded a fragment (inner fragment) of 14,000 daltons with N-terminal serine and the clotting enzyme alpha-thrombin [EC 3.4.21.5], which consists of A and B chains. In the presence of the inhibitors, intermediate ECV and the N-terminal fragment were accumulated in the activation mixture. On the other hand, when prothrombin was activated by the venom activator in the presence of hirudin, antithrombin III, or p-nitrophenyl p'-guanidinobenzoate, it did not yield any fragments but was converted to a derivative with two polypeptide chains having molecular weights of 51,000 and 34,000 daltons, of which the former consisted of N-terminal fragment, the inner fragment, and thrombin A chain, and the latter was thrombin B chain. This new prothrombin derivative, named prothrombin ECV, formed a high-molecular-weight complex, associating with antithrombin III. The complex was not dissociable even in the presence of SDS. Moreover, prothrombin ECV reacted with p-nitrophenyl p'-guanidinobenzoate. On the basis of the results described above, the mechanism of activaton of prothrombin by Echis carinatus venom activator can be summarized as follows: The venom activator first cleaves an Arg-Ile bond liniking thrombin A and B chains in the zymogen molecule, forming an active derivative, prothrombin ECV. This active derivative converts autocatalytically to intermediate ECV, liberating the N-terminal fragment, and active intermediate ECV generates alpha-thrombin, releasing the inner fragment. Thus, only a single peptide bond cleavage along the polypeptide chain of prothrombin is associated with activation by the venom activator...", "contents": "The mechanism of activation of bovine prothrombin by an activator isolated from Echis carinatus venon and characterization of the new active intermediates. Bovine prothrombin was activated, in both the absence and presence of dissopropyphosphofluoridate (DEP) and benzamidine, by an activator which was highly purified from the venom of Echis carinatus (saw-scaled viper, ECV). The process of activation was monitored by sodium dodecysulfate (SDS)-polyacrylamide gel electrophoresis, and the reaction products were isolated and chemically characterized. In the absence of the inhibitors, prothrombin yielded two fragments with molecular weights of 28,000 and 57,000, of which the former was the N-terminal fragment of the zymogen and the latter was intermediate 1, consisting of a single polypeptide chain. Intermediate 1 was subsequently converted to an active intermediate, named intermediate ECV, without decrease of molecular weight. This new intermediate ECV, which showed little clotting activity but a strong alpha-N-tosyl-L-arginine methyl ester (TAME)-esterolytic activity and which bound with hirudin or antithrombin III, consisted of two polypeptide chains with molecular weights of 35,000 of 27,000 daltons. The former was indentified as the thrombin B chain with the N-terminal sequence Ile-Val-Glu-Gly and C-terminal serine, and the latter was a fragment with N-terminal Ser-Gly-Gly, linked to the thrombin A chain. On prolonged incubation, intermediate ECV autocaralytically yielded a fragment (inner fragment) of 14,000 daltons with N-terminal serine and the clotting enzyme alpha-thrombin [EC 3.4.21.5], which consists of A and B chains. In the presence of the inhibitors, intermediate ECV and the N-terminal fragment were accumulated in the activation mixture. On the other hand, when prothrombin was activated by the venom activator in the presence of hirudin, antithrombin III, or p-nitrophenyl p'-guanidinobenzoate, it did not yield any fragments but was converted to a derivative with two polypeptide chains having molecular weights of 51,000 and 34,000 daltons, of which the former consisted of N-terminal fragment, the inner fragment, and thrombin A chain, and the latter was thrombin B chain. This new prothrombin derivative, named prothrombin ECV, formed a high-molecular-weight complex, associating with antithrombin III. The complex was not dissociable even in the presence of SDS. Moreover, prothrombin ECV reacted with p-nitrophenyl p'-guanidinobenzoate. On the basis of the results described above, the mechanism of activaton of prothrombin by Echis carinatus venom activator can be summarized as follows: The venom activator first cleaves an Arg-Ile bond liniking thrombin A and B chains in the zymogen molecule, forming an active derivative, prothrombin ECV. This active derivative converts autocatalytically to intermediate ECV, liberating the N-terminal fragment, and active intermediate ECV generates alpha-thrombin, releasing the inner fragment. Thus, only a single peptide bond cleavage along the polypeptide chain of prothrombin is associated with activation by the venom activator...", "PMID": 956136}
{"id": "pubmed23n0032_2005", "title": "Hybrid enzyme of liver phosphorylase and phosphorylase I.", "content": "A hybrid enzyme (LI) of liver phosphorylase [EC 2.4.1.1.] (L) and phosphorylase I, which is mainly located in brain, was isolated and its enzymatic and immunological properties were examined. The following results were obtained: (1) AMP stimulated the b forms of the hybrid (LIb), I(Ib), and L(Lb); (2) in the presence of AMP, SO42- stimulated Lb more than LIb and inhibited Ib; (3) in the absence of AMP, SO42- stimulated all three isozymes in the order: Ibeta less than LI less than Lbeta; (4) on conversion to the a forms, the activities of L, LI, and I increased 35.5-fold, 3-fold, and 1.2-fold, respectively; (5) the relative inhibition potencies of anti-Lb antibody with LIa and LIb were 63% and 4%, respectively of that with La, and those of anti-Ib antibody with LIa and LIb were 42% and 88%, respectively of that with Ia. Since the ratios of the specific activities of purified La and Ia and of Lbeta and Ibeta are 70: 82 and 2 : 70, respectively (Schliselfeld, 1973), the present findings suggest a 1 : 1 association of I and L subunits in the hybrid molecule.", "contents": "Hybrid enzyme of liver phosphorylase and phosphorylase I. A hybrid enzyme (LI) of liver phosphorylase [EC 2.4.1.1.] (L) and phosphorylase I, which is mainly located in brain, was isolated and its enzymatic and immunological properties were examined. The following results were obtained: (1) AMP stimulated the b forms of the hybrid (LIb), I(Ib), and L(Lb); (2) in the presence of AMP, SO42- stimulated Lb more than LIb and inhibited Ib; (3) in the absence of AMP, SO42- stimulated all three isozymes in the order: Ibeta less than LI less than Lbeta; (4) on conversion to the a forms, the activities of L, LI, and I increased 35.5-fold, 3-fold, and 1.2-fold, respectively; (5) the relative inhibition potencies of anti-Lb antibody with LIa and LIb were 63% and 4%, respectively of that with La, and those of anti-Ib antibody with LIa and LIb were 42% and 88%, respectively of that with Ia. Since the ratios of the specific activities of purified La and Ia and of Lbeta and Ibeta are 70: 82 and 2 : 70, respectively (Schliselfeld, 1973), the present findings suggest a 1 : 1 association of I and L subunits in the hybrid molecule.", "PMID": 956137}
{"id": "pubmed23n0032_2006", "title": "Preparation of monovalent succinyl-concanavalin A and its mitogenic activity.", "content": "Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl a-D-mannopyranoside. This concanavalin A derivate showed a weak but definite mitogenic acitivity against mouse splenic lymphocytes and human peripheral lymphocytes, suggesting that direct receptor cross-linkage on the cell surface is not an obligatory prerequisite for the activation of lymphocytes.", "contents": "Preparation of monovalent succinyl-concanavalin A and its mitogenic activity. Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl a-D-mannopyranoside. This concanavalin A derivate showed a weak but definite mitogenic acitivity against mouse splenic lymphocytes and human peripheral lymphocytes, suggesting that direct receptor cross-linkage on the cell surface is not an obligatory prerequisite for the activation of lymphocytes.", "PMID": 956138}
{"id": "pubmed23n0032_2007", "title": "Ovalbumin synthesis in a homologous cell-free system prepared from hen's oviduct.", "content": "Hen's oviduct polysomes are present in the precipitates prepared from the oviduct homogenates by low-speed centrifugation, in constrast other eukaryotic polysomes. The polysomes possessed synthesizing activity for ovalbumin. The amount of the released form of ovalbumin (soluble ovalbumin) synthesized in cell-free system A or B, consisting of the cell sap and the total ribosomal fraction or the polysomes, respectively, was about a half of that bound to the polysomes (nascent ovalbumin). The amount of soluble ovalbumin synthesized in cell-free system C, consisting of the pH 5 fraction and the polysomes, was only about 5% of that of nascnet ovalbumin. These results indicate that factors required to release nascent ovalbumin from polysomes are present in the pH 5 supernatant fraction. The soluble and nascent ovalbumins, which were purified by chromatography on a CM-cellulose column and by the use of antiovalbumin antiserum, respectively, seemed to be elongation products the initiations of which were supposed to occur in the oviducts before preparation of the cell-free system. The initiated chains in vitro were found to exist as nascent peptides bound to polysomes. Thus, the cell-free systems prepared in the present study lacked the ability to complete initiated peptide chains. The soluble ovalbumin synthesized in the cell-free systems was indentical with ovalbumin A1 containing two residues of phosphates, which was crystallized from hen's egg-white and was different soluble ovalbumin devoid of the prosthetic group (ovalbumin A3) prepared in the oviduct minces. This result suggests that an enzyme necessary for incorporation of the phosphate is present in the cell sap.", "contents": "Ovalbumin synthesis in a homologous cell-free system prepared from hen's oviduct. Hen's oviduct polysomes are present in the precipitates prepared from the oviduct homogenates by low-speed centrifugation, in constrast other eukaryotic polysomes. The polysomes possessed synthesizing activity for ovalbumin. The amount of the released form of ovalbumin (soluble ovalbumin) synthesized in cell-free system A or B, consisting of the cell sap and the total ribosomal fraction or the polysomes, respectively, was about a half of that bound to the polysomes (nascent ovalbumin). The amount of soluble ovalbumin synthesized in cell-free system C, consisting of the pH 5 fraction and the polysomes, was only about 5% of that of nascnet ovalbumin. These results indicate that factors required to release nascent ovalbumin from polysomes are present in the pH 5 supernatant fraction. The soluble and nascent ovalbumins, which were purified by chromatography on a CM-cellulose column and by the use of antiovalbumin antiserum, respectively, seemed to be elongation products the initiations of which were supposed to occur in the oviducts before preparation of the cell-free system. The initiated chains in vitro were found to exist as nascent peptides bound to polysomes. Thus, the cell-free systems prepared in the present study lacked the ability to complete initiated peptide chains. The soluble ovalbumin synthesized in the cell-free systems was indentical with ovalbumin A1 containing two residues of phosphates, which was crystallized from hen's egg-white and was different soluble ovalbumin devoid of the prosthetic group (ovalbumin A3) prepared in the oviduct minces. This result suggests that an enzyme necessary for incorporation of the phosphate is present in the cell sap.", "PMID": 956139}
{"id": "pubmed23n0032_2008", "title": "Biological process of carbohydrate attachment to ovalbumin.", "content": "Dithiothreitol or glutathione, essential for amino acid incorporation into polypeptides in cell-free systems, inhibited glucosamine incorporation into ovalbumin, a glycoprotein containing about three residues of N-acetylucosamine and about five residues of mannose. However, the thiol was necessary for mannose incorporation into ovalbumin in cell-free system prepared from hen's oviducts. The two monosaccharides were incorporated into soluble ovalbumin (intracellular ovalbumin released from the polysomes, corresponding to ovalbumin A3 which contains no phosphate and probably no carbohydrate) present in cell sap which was prepared from homogenates of minced oviducts by ultracentrifugation. It was difficult to study the incorporation of the carbohydrates into ovalbumin in connection with synthesis of the protein using the crude cell-free system. The incorporation of glucosamine and mannose into various forms of ovalbumin was therefore studied using minced oviducts. It was concluded from the incorporation of the two radioactive monsaccharides and of amino acids into the extracellular, soluble and nascent protein fractions that all the carbohydrate moieties were not incorporated into the growing polypeptides bound to polysomes, but were incorporated into ovalbumin molecules released from polysomes after synthesis had been completed.", "contents": "Biological process of carbohydrate attachment to ovalbumin. Dithiothreitol or glutathione, essential for amino acid incorporation into polypeptides in cell-free systems, inhibited glucosamine incorporation into ovalbumin, a glycoprotein containing about three residues of N-acetylucosamine and about five residues of mannose. However, the thiol was necessary for mannose incorporation into ovalbumin in cell-free system prepared from hen's oviducts. The two monosaccharides were incorporated into soluble ovalbumin (intracellular ovalbumin released from the polysomes, corresponding to ovalbumin A3 which contains no phosphate and probably no carbohydrate) present in cell sap which was prepared from homogenates of minced oviducts by ultracentrifugation. It was difficult to study the incorporation of the carbohydrates into ovalbumin in connection with synthesis of the protein using the crude cell-free system. The incorporation of glucosamine and mannose into various forms of ovalbumin was therefore studied using minced oviducts. It was concluded from the incorporation of the two radioactive monsaccharides and of amino acids into the extracellular, soluble and nascent protein fractions that all the carbohydrate moieties were not incorporated into the growing polypeptides bound to polysomes, but were incorporated into ovalbumin molecules released from polysomes after synthesis had been completed.", "PMID": 956140}
{"id": "pubmed23n0032_2009", "title": "Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver.", "content": "1. When the postmitochondrial supernatant (PM-supernatant) from regenerating rat liver was incubated with [3H]methionine, the incorporation of [3H]methinoine into the N-terminal residues of nascent peptides on ribosomes was observed. This incorporation was sensitive to a low cocentration (2X10(-6) M) of pactamycin. The results suggest that PM-supernatant has low but definite activity for the initiation of nascent protein synthesis. Polysomes and cell sap from regenerating rat liver showed negligible pactamycin-sensitive incorporation of [3H]methionine into N-terminal, residues of nascent peptides. 2. PM-supernatant from regenerating rat liver was incubated with [35S]methionine in the complete reaction mixture. After addition of ribosomal proteins labelled with [3H]methionine in vivo, ribosomal structural proteins were prepared from the incubation mixture by acetic acid extraction, CM-cellulose column chromatography, Sephadex G-200 gel filtration and finally by two-dimensional acrylamide gel electrophoresis. Incorporation was observed in the greater part of ribosomal proteins on the two dimensional gel. From the 35S-to-3H ratios of ribsomal protein fractions during the purification procedures, it appeared that the incorporation of labelled methionine into the ribosomal proteins by PM-supernatant was about 3% of that into the total proteins. When [3H]leucine was used, the values were about 4% in the same cell-free system and 5 to 6% in in vivo labelling. The results indicate that ribosomal proteins are synthesized with high efficiency by PM-supernatant from regenerating rat liver.", "contents": "Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. 1. When the postmitochondrial supernatant (PM-supernatant) from regenerating rat liver was incubated with [3H]methionine, the incorporation of [3H]methinoine into the N-terminal residues of nascent peptides on ribosomes was observed. This incorporation was sensitive to a low cocentration (2X10(-6) M) of pactamycin. The results suggest that PM-supernatant has low but definite activity for the initiation of nascent protein synthesis. Polysomes and cell sap from regenerating rat liver showed negligible pactamycin-sensitive incorporation of [3H]methionine into N-terminal, residues of nascent peptides. 2. PM-supernatant from regenerating rat liver was incubated with [35S]methionine in the complete reaction mixture. After addition of ribosomal proteins labelled with [3H]methionine in vivo, ribosomal structural proteins were prepared from the incubation mixture by acetic acid extraction, CM-cellulose column chromatography, Sephadex G-200 gel filtration and finally by two-dimensional acrylamide gel electrophoresis. Incorporation was observed in the greater part of ribosomal proteins on the two dimensional gel. From the 35S-to-3H ratios of ribsomal protein fractions during the purification procedures, it appeared that the incorporation of labelled methionine into the ribosomal proteins by PM-supernatant was about 3% of that into the total proteins. When [3H]leucine was used, the values were about 4% in the same cell-free system and 5 to 6% in in vivo labelling. The results indicate that ribosomal proteins are synthesized with high efficiency by PM-supernatant from regenerating rat liver.", "PMID": 956141}
{"id": "pubmed23n0032_2010", "title": "Effects of polyamines on in vitro dna synthesis by DNA polymerases from calf thymus.", "content": "DNA synthesizing reactions catalyzed both large and small species of calf thymus DNA polymerase (DNA polymerase-alpha and -beta) [EC 2.7.7.7] were stimulated to comparable extents by the presence of spermidine or spermine, and it was not possible to differentiate these two species in terms of their sensitivities to polyamines. Optimal concentrations for stimulation were 0.5-1.0 mM for spermidine and 2-10 mjM for spermine. Excess polyamines strongly inhibited the reactions. The modes of stimulation were as follows: 1) Stimulation was observed with templates bearing long single-stranded sections when either natural DNA or synthetic homopolymer-oligomer duplex was used. 2) The nautral DNA-dependent reaction was stimulated by polyamines at suboptimal concentrations of Mg2+; the apparent Km value for Mg2+ was lowered on adding polyamines, while the Vmax value was unchanged. When synthetic homopolymer-oligomer duplex was used as a template, the reaction was stimulated spermidine even at the optimal concentration of Mn2+. 3) Polyamines markedly influenced the salt requirements of the reactions of DNA polymerase. Spermidine could replace salts such as KC1 or NaC1 at concentrations less than 1/100 of those of salts.", "contents": "Effects of polyamines on in vitro dna synthesis by DNA polymerases from calf thymus. DNA synthesizing reactions catalyzed both large and small species of calf thymus DNA polymerase (DNA polymerase-alpha and -beta) [EC 2.7.7.7] were stimulated to comparable extents by the presence of spermidine or spermine, and it was not possible to differentiate these two species in terms of their sensitivities to polyamines. Optimal concentrations for stimulation were 0.5-1.0 mM for spermidine and 2-10 mjM for spermine. Excess polyamines strongly inhibited the reactions. The modes of stimulation were as follows: 1) Stimulation was observed with templates bearing long single-stranded sections when either natural DNA or synthetic homopolymer-oligomer duplex was used. 2) The nautral DNA-dependent reaction was stimulated by polyamines at suboptimal concentrations of Mg2+; the apparent Km value for Mg2+ was lowered on adding polyamines, while the Vmax value was unchanged. When synthetic homopolymer-oligomer duplex was used as a template, the reaction was stimulated spermidine even at the optimal concentration of Mn2+. 3) Polyamines markedly influenced the salt requirements of the reactions of DNA polymerase. Spermidine could replace salts such as KC1 or NaC1 at concentrations less than 1/100 of those of salts.", "PMID": 956142}
{"id": "pubmed23n0032_2011", "title": "Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens.", "content": "The 3beta-hydroxysteroid oxidase produced by Streptomyces violascens was purified from the culture broth by procedures including batch-wise treatment on DEAE-cellulose, ammonium sulfate fraction, gel filtration on Sephadex G-75, and column chromatography on DEAE-cellulose. The highly purified enzyme preparation exhibited no significant absorption maxima in the visible region other than a maximum at 280 nm. Optimum pH and temperature for the enzyme activity were approximately pH 7.5 and 50 degree, respectively. The Michaelis constant (Km) for cholesterol determined under two different experimental conditions were 4.5 and 6.7 X 10(-4) M. The enzymatic activity was remarkably inhibited by various metal salts such as FeC1(3), FeSO4, AgNO3, etc. On the other hand, neither EDTA nor Fe-chelating agents had any inhibitory effect on the enzymatic activity, while other metal-binding agents, KCN and NaN3, caused significant inhibition. The enzyme activity was inhibited almost completely by N-bromosuccinimide and iodine but not p-chloromercuribenzoate. The highly purified enzyme did not require any external electron acceptors other than oxygen. In addition, the activity was not influenced by the addition of external electron donors. The enzyme showed a high substrate specificity for 3beta-hydroxysteroids and the relative oxidation rates were 100 for cholesterol, 91 for 5alpha-cholestan-3beta-ol, 83 for pregn-5-en3beta-ol-one, 80 for androst-5-en-3beta-ol-17-one, 64 for 5alpha-androstan-3beta-ol-17-one, ect. The oxidation of cholesterol by the enzyme was remarkably inhibited by the addition of 5alpha-cholestan-3beta-ol, 5alpha-cholestan-3-one, 5beta-cholestan-3beta-ol, 5alpha-cholestane3beta, 5alpha-doil or 5alpha-lanosta-8, 24-den 3beta-ol. These findings indicate the present enzyme belongs to the class of 3beta-hydroxysteroid oxidase but some of its physical and enzymatic properties obviously differ from those of 3beta-hyroxysteroid oxidase of Brevibacterium.", "contents": "Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens. The 3beta-hydroxysteroid oxidase produced by Streptomyces violascens was purified from the culture broth by procedures including batch-wise treatment on DEAE-cellulose, ammonium sulfate fraction, gel filtration on Sephadex G-75, and column chromatography on DEAE-cellulose. The highly purified enzyme preparation exhibited no significant absorption maxima in the visible region other than a maximum at 280 nm. Optimum pH and temperature for the enzyme activity were approximately pH 7.5 and 50 degree, respectively. The Michaelis constant (Km) for cholesterol determined under two different experimental conditions were 4.5 and 6.7 X 10(-4) M. The enzymatic activity was remarkably inhibited by various metal salts such as FeC1(3), FeSO4, AgNO3, etc. On the other hand, neither EDTA nor Fe-chelating agents had any inhibitory effect on the enzymatic activity, while other metal-binding agents, KCN and NaN3, caused significant inhibition. The enzyme activity was inhibited almost completely by N-bromosuccinimide and iodine but not p-chloromercuribenzoate. The highly purified enzyme did not require any external electron acceptors other than oxygen. In addition, the activity was not influenced by the addition of external electron donors. The enzyme showed a high substrate specificity for 3beta-hydroxysteroids and the relative oxidation rates were 100 for cholesterol, 91 for 5alpha-cholestan-3beta-ol, 83 for pregn-5-en3beta-ol-one, 80 for androst-5-en-3beta-ol-17-one, 64 for 5alpha-androstan-3beta-ol-17-one, ect. The oxidation of cholesterol by the enzyme was remarkably inhibited by the addition of 5alpha-cholestan-3beta-ol, 5alpha-cholestan-3-one, 5beta-cholestan-3beta-ol, 5alpha-cholestane3beta, 5alpha-doil or 5alpha-lanosta-8, 24-den 3beta-ol. These findings indicate the present enzyme belongs to the class of 3beta-hydroxysteroid oxidase but some of its physical and enzymatic properties obviously differ from those of 3beta-hyroxysteroid oxidase of Brevibacterium.", "PMID": 956143}
{"id": "pubmed23n0032_2012", "title": "Purification and properties of a four iron-four sulfur protein from a Pseudomonas species.", "content": "We have isolated an iron-sulfur proteins from a Pseudomonas species grown on glucose. This protein has different properties from the two known iron-sulfur proteins isolated from other Pseudomonas species: rubredoxin and putidaredoxin. The iron-sulfur protein was purified to homogeneity by DEAE-cellulose column chromatography and Sephadex G-75 gel filtration. The absorption spectrum of the oxidized iron-sulfur protein shows a peak at 283 nm with shoulders at about 290, 320, and 410 nm. The protein contains 4 g atoms of iron and 4 moles of labile sulfur per mole of protein, and has a molecular weight of approximately 14,000. The amino acid composition of the protein shows a predominance of acidic amino acids. The Pseudomonas protein was found to be active for both photosynthetic nicotinamide nucleotide reduction by chloroplasts and cytochrome c reduction by spinach ferredoxin-NADP+ reductase [EC 1.6.7.1]. On the basis of these results, this protein appears to be unique among all known ferredoxins. From an evolutionary point of view, it appears to be more closely related to Azotobacter ferredoxin than to Desulfovibrio ferredoxin.", "contents": "Purification and properties of a four iron-four sulfur protein from a Pseudomonas species. We have isolated an iron-sulfur proteins from a Pseudomonas species grown on glucose. This protein has different properties from the two known iron-sulfur proteins isolated from other Pseudomonas species: rubredoxin and putidaredoxin. The iron-sulfur protein was purified to homogeneity by DEAE-cellulose column chromatography and Sephadex G-75 gel filtration. The absorption spectrum of the oxidized iron-sulfur protein shows a peak at 283 nm with shoulders at about 290, 320, and 410 nm. The protein contains 4 g atoms of iron and 4 moles of labile sulfur per mole of protein, and has a molecular weight of approximately 14,000. The amino acid composition of the protein shows a predominance of acidic amino acids. The Pseudomonas protein was found to be active for both photosynthetic nicotinamide nucleotide reduction by chloroplasts and cytochrome c reduction by spinach ferredoxin-NADP+ reductase [EC 1.6.7.1]. On the basis of these results, this protein appears to be unique among all known ferredoxins. From an evolutionary point of view, it appears to be more closely related to Azotobacter ferredoxin than to Desulfovibrio ferredoxin.", "PMID": 956144}
{"id": "pubmed23n0032_2013", "title": "Interaction of hydrophobic probes with the apoenzyme of pig heart lipoamide dehydrogenase.", "content": "The interaction of hydrophobic probes, 8-anilinonaphthalene-1-sulfonate (ANS) and 4-benzoylamido-4'-aminostilbene-2, 2'-disulfonate (MBAS), with pig heart lipoamide dehydrogenase [NADH: lipoamide oxidoreductase, EC 1.6.4.3] was investigated. When ANS or MBAS was mixed with the apoenzyme of lipoamide dehydrogenase, the fluorescence quantum yield, of each dye was enhancedd markedly and the emission maxima concurrently shifted to the blue. The quantum yield, 0.038, of ANS bound to the apoenzyme, calculated from the corrected emission spectrum, was eight times higher than that in buffer solution, and the value, 0.0090, for bound MBAS was eighteen times higher than that in buffer solution. Moreover, the absortion bands of both ANS and MBAS shifted to the red upon binding with the apoenzyme. A general feature of the absorption spectra of these dyes observed on changing the solvent from polar to apolar was a red shift of the absorption bands. These results indicate that ANS or MBAS bound to the apoenzyme of lipoamide dehydrogenase is situated in a hydrophobic region of the apoenzyme molecule. It was found that 2 moles of each dye was bound per mole of the apoenzyme, which contains two polypeptide chains. The dissociation constants for the ANS- and MBAS-apoenzyme complexes were estimated to be 1.03X10(-5) and 1.54X10(-5) M, respectively. The enhanced fluorescence of both dyes bound to the apoenzyme decreased linearly upon adding FAD and disappeared at about 2 moles of FAD per mole of the apoenzyme. This suggests that both ANS and MBAS were displaced from their binding sites on the apoenzyme by FAD. The protein fluorescence spectrum of the apoenzyme had a maximum at 352 nm, which was blue-shifted by 6 nm from that of tryptophan in the buffer. Upon binding ANS or MBAS, the maximum of the protein fluorescence of the apoenzyme returned to 350 nm for the holoenzyme, and the fluorescence intensity decreased. Thus, the conformation around some tryptophan residues was affected by the binding of the dyes. When guanidine hydrochloride (GuHCl) was added to the ANS-apoenzyme complex solution, the enhanced fluorescence due to the bound ANS decreased and the emission maximum concurrently shifted to the red. Further, the maximum of the protein fluorescence of the apoenzyme shifted to the red, indicating the exposure of some tryptophan residues buried in an apolar region of the apoenzyme. Thus the binding of ANS to the apoenzyme was inhibited by protein denaturation due to GuHCL. In contrast, the holoenzyme of lipoamide dehydrogenase did not bind ANS or MBAS at all.", "contents": "Interaction of hydrophobic probes with the apoenzyme of pig heart lipoamide dehydrogenase. The interaction of hydrophobic probes, 8-anilinonaphthalene-1-sulfonate (ANS) and 4-benzoylamido-4'-aminostilbene-2, 2'-disulfonate (MBAS), with pig heart lipoamide dehydrogenase [NADH: lipoamide oxidoreductase, EC 1.6.4.3] was investigated. When ANS or MBAS was mixed with the apoenzyme of lipoamide dehydrogenase, the fluorescence quantum yield, of each dye was enhancedd markedly and the emission maxima concurrently shifted to the blue. The quantum yield, 0.038, of ANS bound to the apoenzyme, calculated from the corrected emission spectrum, was eight times higher than that in buffer solution, and the value, 0.0090, for bound MBAS was eighteen times higher than that in buffer solution. Moreover, the absortion bands of both ANS and MBAS shifted to the red upon binding with the apoenzyme. A general feature of the absorption spectra of these dyes observed on changing the solvent from polar to apolar was a red shift of the absorption bands. These results indicate that ANS or MBAS bound to the apoenzyme of lipoamide dehydrogenase is situated in a hydrophobic region of the apoenzyme molecule. It was found that 2 moles of each dye was bound per mole of the apoenzyme, which contains two polypeptide chains. The dissociation constants for the ANS- and MBAS-apoenzyme complexes were estimated to be 1.03X10(-5) and 1.54X10(-5) M, respectively. The enhanced fluorescence of both dyes bound to the apoenzyme decreased linearly upon adding FAD and disappeared at about 2 moles of FAD per mole of the apoenzyme. This suggests that both ANS and MBAS were displaced from their binding sites on the apoenzyme by FAD. The protein fluorescence spectrum of the apoenzyme had a maximum at 352 nm, which was blue-shifted by 6 nm from that of tryptophan in the buffer. Upon binding ANS or MBAS, the maximum of the protein fluorescence of the apoenzyme returned to 350 nm for the holoenzyme, and the fluorescence intensity decreased. Thus, the conformation around some tryptophan residues was affected by the binding of the dyes. When guanidine hydrochloride (GuHCl) was added to the ANS-apoenzyme complex solution, the enhanced fluorescence due to the bound ANS decreased and the emission maximum concurrently shifted to the red. Further, the maximum of the protein fluorescence of the apoenzyme shifted to the red, indicating the exposure of some tryptophan residues buried in an apolar region of the apoenzyme. Thus the binding of ANS to the apoenzyme was inhibited by protein denaturation due to GuHCL. In contrast, the holoenzyme of lipoamide dehydrogenase did not bind ANS or MBAS at all.", "PMID": 956145}
{"id": "pubmed23n0032_2014", "title": "Synergistic action of two different types of endo-cellulase components from Irpex lacteus (Polyporus tulipiferae) in the hydrolysis of some insoluble celluloses.", "content": "The substrate specificities of three endo-cellulase [EC 3.2.1.4] components, F-1, F-2, and S-1, obtained from the culture filtrate of Irpex lacteus (Polyporus tulipiferae), were investigated in detail. It was confirmed that the former is of a more random type, belonging to the carboxymethyl-cellulase (CMCcase) group, and the latter two are of a less random type, beloning to the Avicelase group. It was found that a mixture of CMCase and Avicelase shows a remarkable synergistic action in the degradation of cotton and Avicel and that CMCase lowers the degree of polymerization of both cotton and CM-cellulose faster than Avicelases, relative to the production of reducing sugar. Thus, it was assumed that cotton and similar cellulosic substrates were degraded mainly by the synergistic action of these cellulase components produced by this celluloytic fungus.", "contents": "Synergistic action of two different types of endo-cellulase components from Irpex lacteus (Polyporus tulipiferae) in the hydrolysis of some insoluble celluloses. The substrate specificities of three endo-cellulase [EC 3.2.1.4] components, F-1, F-2, and S-1, obtained from the culture filtrate of Irpex lacteus (Polyporus tulipiferae), were investigated in detail. It was confirmed that the former is of a more random type, belonging to the carboxymethyl-cellulase (CMCcase) group, and the latter two are of a less random type, beloning to the Avicelase group. It was found that a mixture of CMCase and Avicelase shows a remarkable synergistic action in the degradation of cotton and Avicel and that CMCase lowers the degree of polymerization of both cotton and CM-cellulose faster than Avicelases, relative to the production of reducing sugar. Thus, it was assumed that cotton and similar cellulosic substrates were degraded mainly by the synergistic action of these cellulase components produced by this celluloytic fungus.", "PMID": 956146}
{"id": "pubmed23n0032_2015", "title": "Effect of albumin and methylated albumin on the glucose permeability of lipid membranes.", "content": "The diffusion of glucose from phospholipid membranes has been measured in the presence of serum albumin or methylated serum albumin. At neutral pH, serum albumin enhanced the rate at which glucose diffused from liposomes containing more than a certain amount of lysolecithin. Net charge of the membrane is not important for the reaction, since positively charged membranes containing stearylamine showed almost the same reactivity as negatively charged liposomes containing dicetyl phosphate. Carboxylmethylated albumin showed enhancement of the diffusion rate of glucose from negatively but not from positively charged liposomes. The amount of methylated albumin required to affect liposomes was much smaller than the amount of albumin required to damage liposomes containing lysolecithin. Cholesterol incorporation suppressed the sensitivity of liposomes to both proteins, albumin and methylated albumin. The effect of temperature and fatty acid composition of phospholipids on the sensitivity of liposomes to proteins suggests the importance of the fluidity of the membrane, especially in the case of methylated albumin.", "contents": "Effect of albumin and methylated albumin on the glucose permeability of lipid membranes. The diffusion of glucose from phospholipid membranes has been measured in the presence of serum albumin or methylated serum albumin. At neutral pH, serum albumin enhanced the rate at which glucose diffused from liposomes containing more than a certain amount of lysolecithin. Net charge of the membrane is not important for the reaction, since positively charged membranes containing stearylamine showed almost the same reactivity as negatively charged liposomes containing dicetyl phosphate. Carboxylmethylated albumin showed enhancement of the diffusion rate of glucose from negatively but not from positively charged liposomes. The amount of methylated albumin required to affect liposomes was much smaller than the amount of albumin required to damage liposomes containing lysolecithin. Cholesterol incorporation suppressed the sensitivity of liposomes to both proteins, albumin and methylated albumin. The effect of temperature and fatty acid composition of phospholipids on the sensitivity of liposomes to proteins suggests the importance of the fluidity of the membrane, especially in the case of methylated albumin.", "PMID": 956147}
{"id": "pubmed23n0032_2016", "title": "Properties of liposomal membranes composed of short-chain lecithins.", "content": "Permeability properties of multilamellar liposomes prepared from synthetic, saturated short-chain (C8, C10, C12) lecithins (3-sn-phosphatidyl choline) were studied. Dioctanoyllecithin, didecanoyllecithin, and dilauroyllecithin form \"stable\" bilayers which are practically impermeable to glucose when prepared with proportions of more than 1.2, 0.75, and 0.6 of cholesterol (molar ratio to phospholipid), respectively. Dioctanoyllecithin liposomes were rather leaky above 30 degrees, even when a proportion of 1.5 of cholesterol (molar ratio to lecithin) was incorporated. Judging from their sensitivities to temperature, Triton X-100, serum albumin, and other reagents, short-chain lecithin bilayers show characteristic properties, different from those of both saturated long-chain lecithins (dipalmitoyllecithin and dimyristoyllecithin) and lecithins having unsaturated fatty acid residues (egg lecithin and dioleoyllecithin).", "contents": "Properties of liposomal membranes composed of short-chain lecithins. Permeability properties of multilamellar liposomes prepared from synthetic, saturated short-chain (C8, C10, C12) lecithins (3-sn-phosphatidyl choline) were studied. Dioctanoyllecithin, didecanoyllecithin, and dilauroyllecithin form \"stable\" bilayers which are practically impermeable to glucose when prepared with proportions of more than 1.2, 0.75, and 0.6 of cholesterol (molar ratio to phospholipid), respectively. Dioctanoyllecithin liposomes were rather leaky above 30 degrees, even when a proportion of 1.5 of cholesterol (molar ratio to lecithin) was incorporated. Judging from their sensitivities to temperature, Triton X-100, serum albumin, and other reagents, short-chain lecithin bilayers show characteristic properties, different from those of both saturated long-chain lecithins (dipalmitoyllecithin and dimyristoyllecithin) and lecithins having unsaturated fatty acid residues (egg lecithin and dioleoyllecithin).", "PMID": 956148}
{"id": "pubmed23n0032_2017", "title": "Fragmentation of rat serum albumin by cyanogen bromide cleavage and the amino acid sequences of four fragments.", "content": "Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.", "contents": "Fragmentation of rat serum albumin by cyanogen bromide cleavage and the amino acid sequences of four fragments. Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.", "PMID": 956149}
{"id": "pubmed23n0032_2018", "title": "Fluorometric method for assay of kallikrein-like arginine esterases.", "content": "The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-a-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromotropic acid, and 3-methyl-2-benzothiazolone hydrazone and the present method was more sensitive than these colorimetric methods.", "contents": "Fluorometric method for assay of kallikrein-like arginine esterases. The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-a-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromotropic acid, and 3-methyl-2-benzothiazolone hydrazone and the present method was more sensitive than these colorimetric methods.", "PMID": 956150}
{"id": "pubmed23n0032_2019", "title": "Primary structure of bovine plasma high-molecular-weight kininogen. The amino acid sequence of a glycopeptide portion (fragment 1) following the C-terminus ot the bradykinin moiety.", "content": "On incubation of bovine plasma high-molecular-weight (HMW) kininogen with purified plasma kallikrein [EC 3.4.21.8], a large glycopeptide fragment and the vasoactive peptide, bradykinin, were initially liberated; the former, named fragment 1-2, was subsequently cleaved into fragment 1 (glycopeptide) and the previously established fragment 2 (histidine-rich peptide). The isolated fragment 1-2 contained a total of 108 to 110 amino acid residues and carbohydrates, and the amino-terminal sequence Ser-Val-Gln was established. The other fragment, fragment 1, consisted of a total of 69 amino acid residues with serine and arginine (and lysine) at the amino and carboxyl termini, respectively. It contained eleven residues each of histidine and glycine, together with an oligosaccharide chain consisting of galactosamine, hexose and sialic acid. The complete amino acid sequence of fragment 1 was determined by Edman degradation and standard enzymatic and chemical techniques. These results established the following sequence: H-Ser-Val-Gln-Val-Met-Lys-Thr-Glu-Gly-Ser-Thr-Pro/Thr-Val-Ser(CHO)-Val/Leu-Pro-His-Ser-Ala-Met-Ser-Pro-Val-Gln-Asp-Glu-Glu-Arg-Asp-Ser-Gly-Lys-Glu-Gln-Gly-Pro-Thr-His-Gly-His-Gly-Trp-Asp-His-Gly-Lys-Gln-Ile-Lys-Leu-His-Gly-Leu-Gly-Leu-Gly-His-Lys-His-Lys-His-Asp-Gln-Gly-His-Gly-His-His-Lys/ArgOH.", "contents": "Primary structure of bovine plasma high-molecular-weight kininogen. The amino acid sequence of a glycopeptide portion (fragment 1) following the C-terminus ot the bradykinin moiety. On incubation of bovine plasma high-molecular-weight (HMW) kininogen with purified plasma kallikrein [EC 3.4.21.8], a large glycopeptide fragment and the vasoactive peptide, bradykinin, were initially liberated; the former, named fragment 1-2, was subsequently cleaved into fragment 1 (glycopeptide) and the previously established fragment 2 (histidine-rich peptide). The isolated fragment 1-2 contained a total of 108 to 110 amino acid residues and carbohydrates, and the amino-terminal sequence Ser-Val-Gln was established. The other fragment, fragment 1, consisted of a total of 69 amino acid residues with serine and arginine (and lysine) at the amino and carboxyl termini, respectively. It contained eleven residues each of histidine and glycine, together with an oligosaccharide chain consisting of galactosamine, hexose and sialic acid. The complete amino acid sequence of fragment 1 was determined by Edman degradation and standard enzymatic and chemical techniques. These results established the following sequence: H-Ser-Val-Gln-Val-Met-Lys-Thr-Glu-Gly-Ser-Thr-Pro/Thr-Val-Ser(CHO)-Val/Leu-Pro-His-Ser-Ala-Met-Ser-Pro-Val-Gln-Asp-Glu-Glu-Arg-Asp-Ser-Gly-Lys-Glu-Gln-Gly-Pro-Thr-His-Gly-His-Gly-Trp-Asp-His-Gly-Lys-Gln-Ile-Lys-Leu-His-Gly-Leu-Gly-Leu-Gly-His-Lys-His-Lys-His-Asp-Gln-Gly-His-Gly-His-His-Lys/ArgOH.", "PMID": 956151}
{"id": "pubmed23n0032_2020", "title": "Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.", "content": "Fruit bromelain FA2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized. 1. Efficient extraction of this enzyme from the crude material was possible using \"Cellulosin AP,\" a microbial polysaccharidase preparation containing cellulase, hemicellulase, and pectinase. The enzyme was purified mainly by successive applications of anion-exchange chromatography, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunochemical criteria. Properties of FA2 include: molecular weight, 31,000; isoelectric point, pH 4.6; absorbance at 280 nm of a 1% solution at pH 7.0 per cm, 19.2. 2. FA2 gave only alanine phenylthiohydantoin upon amino-terminal group analysis by the Edman procedure. Stepwise degradation yielded the amino-terminal sequence Ala-Val-Pro-Gln-Ser-Ile-Asp-Trp-Arg-Asp-Tyr-Gly-Ala. The amino acid composition of FA2 was not markedly different from that of stem bromelain, except for a much smaller lysine content and a smaller alanine content relative to glycine in FA2. FA2 contained neither amino sugars nor neutral carbohydrates as determined by several methods, so FA2 is not a glycoprotein. 3. By labeling the reactive cysteine residue (CYS) with [14C]iodoacetate, the following partial amino acid sequence has been determined. Asn-Glx-Asn-Pro-Cys-Gly-Ala-CYS.", "contents": "Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2. Fruit bromelain FA2, the main proteinase component of the juice of pineapple fruit, has been purified and characterized. 1. Efficient extraction of this enzyme from the crude material was possible using \"Cellulosin AP,\" a microbial polysaccharidase preparation containing cellulase, hemicellulase, and pectinase. The enzyme was purified mainly by successive applications of anion-exchange chromatography, yielding an apparently homogeneous protein as judged by several physical, chemical, and immunochemical criteria. Properties of FA2 include: molecular weight, 31,000; isoelectric point, pH 4.6; absorbance at 280 nm of a 1% solution at pH 7.0 per cm, 19.2. 2. FA2 gave only alanine phenylthiohydantoin upon amino-terminal group analysis by the Edman procedure. Stepwise degradation yielded the amino-terminal sequence Ala-Val-Pro-Gln-Ser-Ile-Asp-Trp-Arg-Asp-Tyr-Gly-Ala. The amino acid composition of FA2 was not markedly different from that of stem bromelain, except for a much smaller lysine content and a smaller alanine content relative to glycine in FA2. FA2 contained neither amino sugars nor neutral carbohydrates as determined by several methods, so FA2 is not a glycoprotein. 3. By labeling the reactive cysteine residue (CYS) with [14C]iodoacetate, the following partial amino acid sequence has been determined. Asn-Glx-Asn-Pro-Cys-Gly-Ala-CYS.", "PMID": 956152}
{"id": "pubmed23n0032_2021", "title": "Protein synthesis in a cell-free system from an extreme thermophile. Effects of preincubation in the cold on polyuridylic acid-dependent polyphenylalanine synthesis at high temperature.", "content": "1. It was found that preincubation of the reaction mixture in the cold enhanced polyuridylic acid-directed polyphenylalanine synthesis by a cell-free extract of Thermus thermophilus HB8 at high temperature. 2. The effect of preincubation was most marked at 10-25 degrees in the presence of 20 mM Mg2+. Preincubation at 65 degrees failed to stimulate the incorporation. 3. The presence of phenylalanyl-tRNA, polyuridylic acid, and ribosomes was essential for preincubation in the cold to be effective. 4. A ternary complex of amino acyl-tRNA, polyuridylic acid, and a ribosome formed at low temperature was isolated by CPG-10 column chromatography; the isolated complex initiated polyphenylalanine synthesis effectively at high temperature. 5. The amount of the ternary complex formed depends on the preincubation time and the concentration of Mg2+. Since the amount of the complex correlated positively to the rate of polyphenylalanine synthesis at high temperature, the effectiveness of preincubation in the cold is presumably due to the formation of the ternary complex of phenylalanyl-tRNA, polyuridylic acid, and a ribosome.", "contents": "Protein synthesis in a cell-free system from an extreme thermophile. Effects of preincubation in the cold on polyuridylic acid-dependent polyphenylalanine synthesis at high temperature. 1. It was found that preincubation of the reaction mixture in the cold enhanced polyuridylic acid-directed polyphenylalanine synthesis by a cell-free extract of Thermus thermophilus HB8 at high temperature. 2. The effect of preincubation was most marked at 10-25 degrees in the presence of 20 mM Mg2+. Preincubation at 65 degrees failed to stimulate the incorporation. 3. The presence of phenylalanyl-tRNA, polyuridylic acid, and ribosomes was essential for preincubation in the cold to be effective. 4. A ternary complex of amino acyl-tRNA, polyuridylic acid, and a ribosome formed at low temperature was isolated by CPG-10 column chromatography; the isolated complex initiated polyphenylalanine synthesis effectively at high temperature. 5. The amount of the ternary complex formed depends on the preincubation time and the concentration of Mg2+. Since the amount of the complex correlated positively to the rate of polyphenylalanine synthesis at high temperature, the effectiveness of preincubation in the cold is presumably due to the formation of the ternary complex of phenylalanyl-tRNA, polyuridylic acid, and a ribosome.", "PMID": 956153}
{"id": "pubmed23n0032_2022", "title": "Properties and subunit structure of pig heart pyruvate dehydrogenase.", "content": "Pyruvate dehydrogenase [EC 1.2.4.1] was separated from the pyruvate dehydrogenase complex and its molecular weight was estimated to be about 150,000 by sedimentation equilibrium methods. The enzyme was dissociated into two subunits (alpha and beta), with estimated molecular weights of 41,000 (alpha) and 36,000 (beta), respectively, by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The subunits were separated by phosphocellulose column chromatography and their chemical properties were examined. The subunit structure of the pyruvate dehydrogenase was assigned as alpha2beta2. The content of right-handed alpha-helix in the enzyme molecule was estimated to be about 29 and 28% by optical rotatory dispersion and by circular dichroism, respectively. The enzyme contained no thiamine-PP, and its dehydrogenase activity was completely dependent on added thiamine-PP and partially dependent on added Mg2+ and Ca2+. The Km value of pyruvate dehydrogenase for thiamine diphosphate was estimated to be 6.5 X 10(-5) M in the presence of Mg2+ or Ca2+. The enzyme showed highly specific activity for thiamine-PP dependent oxidation of both pyruvate and alpha-ketobutyrate, but it also showed some activity with alpha-ketovalerate, alpha-ketoisocaproate, and alpha-ketoisovalerate. The pyruvate dehydrogenase activity was strongly inhibited by bivalent heavy metal ions and by sulfhydryl inhibitors; and the enzyme molecule contained 27 moles of 5,5'-dithiobis(2-nitrobenzoic acid)-reactive sulfhydryl groups and a total of 36 moles of sulfhydryl groups. The inhibitory effect of p-chloromercuribenzoate was prevented by preincubating the enzyme with thiamine-PP plus pyruvate. The structure of pyruvate dehydrogenase necessary for formation of the complex is also reported.", "contents": "Properties and subunit structure of pig heart pyruvate dehydrogenase. Pyruvate dehydrogenase [EC 1.2.4.1] was separated from the pyruvate dehydrogenase complex and its molecular weight was estimated to be about 150,000 by sedimentation equilibrium methods. The enzyme was dissociated into two subunits (alpha and beta), with estimated molecular weights of 41,000 (alpha) and 36,000 (beta), respectively, by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The subunits were separated by phosphocellulose column chromatography and their chemical properties were examined. The subunit structure of the pyruvate dehydrogenase was assigned as alpha2beta2. The content of right-handed alpha-helix in the enzyme molecule was estimated to be about 29 and 28% by optical rotatory dispersion and by circular dichroism, respectively. The enzyme contained no thiamine-PP, and its dehydrogenase activity was completely dependent on added thiamine-PP and partially dependent on added Mg2+ and Ca2+. The Km value of pyruvate dehydrogenase for thiamine diphosphate was estimated to be 6.5 X 10(-5) M in the presence of Mg2+ or Ca2+. The enzyme showed highly specific activity for thiamine-PP dependent oxidation of both pyruvate and alpha-ketobutyrate, but it also showed some activity with alpha-ketovalerate, alpha-ketoisocaproate, and alpha-ketoisovalerate. The pyruvate dehydrogenase activity was strongly inhibited by bivalent heavy metal ions and by sulfhydryl inhibitors; and the enzyme molecule contained 27 moles of 5,5'-dithiobis(2-nitrobenzoic acid)-reactive sulfhydryl groups and a total of 36 moles of sulfhydryl groups. The inhibitory effect of p-chloromercuribenzoate was prevented by preincubating the enzyme with thiamine-PP plus pyruvate. The structure of pyruvate dehydrogenase necessary for formation of the complex is also reported.", "PMID": 956154}
{"id": "pubmed23n0032_2023", "title": "Hybridization of glyceraldehyde-3-phosphate dehydrogenase in borate.", "content": "Conditions for the hybridization of glyceraldehyde-3-phosphate dehydrogenase (GPD) [EC 1.2.1.12] in the presence of dilute borate were examined with horseshoe crab and rabbit GPDs. Hybridization was strongly dependent upon pH, borate concentration, and temperature. The optimum medium for hybridization was 10mM Tris-HCl-1 mM 2-mercaptoethanol-1mM EDTA containing 10-20 mM borate, pH 8.5-9.0. Hybridization was performed by incubation of two electrophoretically distinct GPDs at 30 degrees for 3-15 hr in the above medium at a protein concentration of 1-2 mg/ml. The time course of hybridization was analyzed under the optimized conditions. Symmetrical A2B2-type hybrid appeared only 5 min after incubation for 1 hr. Hybridization of GPDs from 7 different species was examined under the optimal conditions. Hybridization was detected with rabbit-horseshoe crab, yeast-rabbit, yeast-chicken, and chicken-horseshoe crab combinations. Subunit-subunit interaction, the mechanism of hybridization, and the structure of GPD are discussed based on the results obtained.", "contents": "Hybridization of glyceraldehyde-3-phosphate dehydrogenase in borate. Conditions for the hybridization of glyceraldehyde-3-phosphate dehydrogenase (GPD) [EC 1.2.1.12] in the presence of dilute borate were examined with horseshoe crab and rabbit GPDs. Hybridization was strongly dependent upon pH, borate concentration, and temperature. The optimum medium for hybridization was 10mM Tris-HCl-1 mM 2-mercaptoethanol-1mM EDTA containing 10-20 mM borate, pH 8.5-9.0. Hybridization was performed by incubation of two electrophoretically distinct GPDs at 30 degrees for 3-15 hr in the above medium at a protein concentration of 1-2 mg/ml. The time course of hybridization was analyzed under the optimized conditions. Symmetrical A2B2-type hybrid appeared only 5 min after incubation for 1 hr. Hybridization of GPDs from 7 different species was examined under the optimal conditions. Hybridization was detected with rabbit-horseshoe crab, yeast-rabbit, yeast-chicken, and chicken-horseshoe crab combinations. Subunit-subunit interaction, the mechanism of hybridization, and the structure of GPD are discussed based on the results obtained.", "PMID": 956155}
{"id": "pubmed23n0032_2024", "title": "A simple fluorometric assay for type B monoamine oxidase activity in rat tissues.", "content": "A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4] activity towards beta-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10 mg to 100 mg of liver tissue in 3 ml of reaction mixture for up to 90 min of incubation. Using this method, the V max values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).", "contents": "A simple fluorometric assay for type B monoamine oxidase activity in rat tissues. A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4] activity towards beta-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10 mg to 100 mg of liver tissue in 3 ml of reaction mixture for up to 90 min of incubation. Using this method, the V max values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).", "PMID": 956156}
{"id": "pubmed23n0032_2025", "title": "Effect of priming doses of chemically modified antigen on helper activity.", "content": "Mice primed with chemically modified bacterial alpha-amylase (BalphaA) [EC 3.2.1.1 alpha-amylase, B. subtilis], which was neither cross-reactive with anti-BalphaA antibody nor able to induce a humoral anti-BalphaA antibody response, developed enhanced responses to a subsequent challenge with native BalphaA (Nakashima et al. (1974) J. Biochem. 76, 349-357). The present studies were designed to examine the relationship of priming doses of BalphaA derivatives to the level of enhancement of the helper activity. Increasing the priming dose of modified antigens resulted in a greater degree of helper cell response until the maximal level of enhancement was reached. When injections for priming and challenge were given intraperitoneally, priming doses of D-BalphaA, M-BalphaA, and RM-BalphaA required for the maximal enhancement of helper activity were about 15, 50, and 15 mug, respectively. Further increase in the priming dose, conversely, resulted in suppressin of the enhanced helper activity, irrespective of whether the time interval between priming and challenge was 10 or 28 days. Suppression of the enhanced helper activity upon excessive dose priming with modified BalphaA derivatives was not specific for the anti-BalphaA antibody response. On the basis of these results it is suggested that this phenomenon of suppression might be partly accounted for by the regulatory mechanism functioning in antigenic competition.", "contents": "Effect of priming doses of chemically modified antigen on helper activity. Mice primed with chemically modified bacterial alpha-amylase (BalphaA) [EC 3.2.1.1 alpha-amylase, B. subtilis], which was neither cross-reactive with anti-BalphaA antibody nor able to induce a humoral anti-BalphaA antibody response, developed enhanced responses to a subsequent challenge with native BalphaA (Nakashima et al. (1974) J. Biochem. 76, 349-357). The present studies were designed to examine the relationship of priming doses of BalphaA derivatives to the level of enhancement of the helper activity. Increasing the priming dose of modified antigens resulted in a greater degree of helper cell response until the maximal level of enhancement was reached. When injections for priming and challenge were given intraperitoneally, priming doses of D-BalphaA, M-BalphaA, and RM-BalphaA required for the maximal enhancement of helper activity were about 15, 50, and 15 mug, respectively. Further increase in the priming dose, conversely, resulted in suppressin of the enhanced helper activity, irrespective of whether the time interval between priming and challenge was 10 or 28 days. Suppression of the enhanced helper activity upon excessive dose priming with modified BalphaA derivatives was not specific for the anti-BalphaA antibody response. On the basis of these results it is suggested that this phenomenon of suppression might be partly accounted for by the regulatory mechanism functioning in antigenic competition.", "PMID": 956157}
{"id": "pubmed23n0032_2026", "title": "The effect of temperature on ribose-5-phosphate isomerase from a mesophile, Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus.", "content": "The enzyme ribose-5-phosphate isomerase [EC 5.3.1.6] was partially purified from a mesophilic organism, Thiobacillus thioparus, and from an extreme thermophile, Bacillus caldolyticus. The stability and kinetics of the two enzymes were compared with regard to temperature in the presence of a series of neutral salts and alcohols. The thermal stability of both enzymes was altered such that the salts (NH4)2SO4, NaCl, KCl, and LiCl increased stability, while LiBr, CaCl2, methanol, ethanol, and 1-propanol decreased stability. Ethylene glycol had little effect on the mesophilic enzyme, but increased the stability of the thermophilic protein. The kinetics of both enzymes were also affected by the salts and alcohols, and Arrhenius plots of two kinetic parameters, Km and Vmax, displayed discontinuities, or sharp changes in slope, at characteristic temperatures, TD. Neutral salts and alcohols altered the temperature of discontinuity in a sequence similar to that observed in studies of thermal stability. It is suggested that the slope change is due to temperature-dependent alterations in the enzymes at specific, but undefined, loci at the active site, although no evidence is offered for the absence of a larger conformation change in the entire enzyme.", "contents": "The effect of temperature on ribose-5-phosphate isomerase from a mesophile, Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus. The enzyme ribose-5-phosphate isomerase [EC 5.3.1.6] was partially purified from a mesophilic organism, Thiobacillus thioparus, and from an extreme thermophile, Bacillus caldolyticus. The stability and kinetics of the two enzymes were compared with regard to temperature in the presence of a series of neutral salts and alcohols. The thermal stability of both enzymes was altered such that the salts (NH4)2SO4, NaCl, KCl, and LiCl increased stability, while LiBr, CaCl2, methanol, ethanol, and 1-propanol decreased stability. Ethylene glycol had little effect on the mesophilic enzyme, but increased the stability of the thermophilic protein. The kinetics of both enzymes were also affected by the salts and alcohols, and Arrhenius plots of two kinetic parameters, Km and Vmax, displayed discontinuities, or sharp changes in slope, at characteristic temperatures, TD. Neutral salts and alcohols altered the temperature of discontinuity in a sequence similar to that observed in studies of thermal stability. It is suggested that the slope change is due to temperature-dependent alterations in the enzymes at specific, but undefined, loci at the active site, although no evidence is offered for the absence of a larger conformation change in the entire enzyme.", "PMID": 956158}
{"id": "pubmed23n0032_2027", "title": "Macromolecular components of the vitelline membrane of hen's egg. II. Physicochemical properties of glycoprotein I.", "content": "Of the three major macromolecular components of the vitelline membrane of hen's egg, the lowest molecular weight component (previously designated component I) has been studied and its physicochemical properties clarified. The molecular weight of this component is 27,000 and its chemical composition is typical of a glycoprotein, consisting of protein (91%), total hexose (4.4%), hexosamine (glucosamine 2.3%; galactosamine 0.7%), and sialic acid (1.7%). Uronic acid was not found. The molar ratios of the constituent neutral sugars of this glycoprotein (GP-1) are as follows: fucose 3, mannose 5, galactose 5, glucose 1, and xylose 1. The amino acid profile shows a relatively high proportion of hydrophobic amino acids (39%), which may partly account for the insolubility of GP-I in water.", "contents": "Macromolecular components of the vitelline membrane of hen's egg. II. Physicochemical properties of glycoprotein I. Of the three major macromolecular components of the vitelline membrane of hen's egg, the lowest molecular weight component (previously designated component I) has been studied and its physicochemical properties clarified. The molecular weight of this component is 27,000 and its chemical composition is typical of a glycoprotein, consisting of protein (91%), total hexose (4.4%), hexosamine (glucosamine 2.3%; galactosamine 0.7%), and sialic acid (1.7%). Uronic acid was not found. The molar ratios of the constituent neutral sugars of this glycoprotein (GP-1) are as follows: fucose 3, mannose 5, galactose 5, glucose 1, and xylose 1. The amino acid profile shows a relatively high proportion of hydrophobic amino acids (39%), which may partly account for the insolubility of GP-I in water.", "PMID": 956159}
{"id": "pubmed23n0032_2028", "title": "Roles of nucleoside triphosphates in microtubule assembly.", "content": "Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruvate and pyruvate kinase [EC 2.7.1.40] also induced polymerization. Depolymerization of microtubules in the GTP-reassembly buffer yielded GTPETNGTP, which was capable of polymerizing into microtubules even in the absence of free GTP. In the presence of 4 M glycerol, GDPETNGTP assembled into microtubules with no change in the bound nucleotides.", "contents": "Roles of nucleoside triphosphates in microtubule assembly. Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruvate and pyruvate kinase [EC 2.7.1.40] also induced polymerization. Depolymerization of microtubules in the GTP-reassembly buffer yielded GTPETNGTP, which was capable of polymerizing into microtubules even in the absence of free GTP. In the presence of 4 M glycerol, GDPETNGTP assembled into microtubules with no change in the bound nucleotides.", "PMID": 956160}
{"id": "pubmed23n0032_2029", "title": "Biostereometrics and the communication of biological form.", "content": "Biostereometrics-the spatial and spatio-temporal analysis of biological form and function based on principles geometry-is a modern approach to the measurement and analysis of biological form which recognizes that organic structures are irregular and three-dimensional (or four-dimensional, as in movement or growth). Stereometric sensors of various types are used to determine the coordinates of points distributed over the surface (internal or external). The resulting data provide a more comprehensive, parsimonious and unambiguous spatial quantification than heretofore possible using traditional lengths, breadths, and circumferences. Recent and prospective biomedical applications are described.", "contents": "Biostereometrics and the communication of biological form. Biostereometrics-the spatial and spatio-temporal analysis of biological form and function based on principles geometry-is a modern approach to the measurement and analysis of biological form which recognizes that organic structures are irregular and three-dimensional (or four-dimensional, as in movement or growth). Stereometric sensors of various types are used to determine the coordinates of points distributed over the surface (internal or external). The resulting data provide a more comprehensive, parsimonious and unambiguous spatial quantification than heretofore possible using traditional lengths, breadths, and circumferences. Recent and prospective biomedical applications are described.", "PMID": 956161}
{"id": "pubmed23n0032_2030", "title": "Steroidogenesis in the zona glomerulosa of the adrenal coretx. I. Isolation and some properties of mitochondria from the zona glomerulosa of the bovine adrenal cortex.", "content": "Isolation procedures for mitochondria from the zona glomerulosa of the bovine adrenal cortex are described and the properties of the mitochondria thus prepared are compared with those isolated from the zona fasciculoreticularis. The cristal membranes of mitochondria in the zona glomerulosa in situ are tubular or tubulovesicular, whereas those of mitochondria in the zona fasciculoreticularis in situ are vesicular. When mitochondria are isolated from the former zone, they invariably showed the condensed configuration regardless of isolation media, whereas those isolated from the latter zone in an ST medium showed the orthodox configuration. When Ca2+ was added to mitochondria isolated either from the zona glomerulosa or the zona fasciculoreticularis in an STE medium in the condensed configuration, a transition from the condensed to the orthodox configuration tool place; the cristal membranes of mitochondria from the zona glomerulosa became tubular or tubulovesicular and those of mitochondria from the zona fasciculoreticularis became vesicular. Contaminations of mitrochondria of the zona glomerulosa with other cellular organelles were examined using various marker enzymes. There was no difference in cytochrome content between mitochondria of the two zones specified above. The coupling efficiency of mitochondria of the zona glomerulosa was found to be remarkably effected by temperature during the isolation procedures. Effects of various substrates, isolation media, and bovine serum albumin on the coupling efficiency of mitochondria of the zona glomerulosa are also described.", "contents": "Steroidogenesis in the zona glomerulosa of the adrenal coretx. I. Isolation and some properties of mitochondria from the zona glomerulosa of the bovine adrenal cortex. Isolation procedures for mitochondria from the zona glomerulosa of the bovine adrenal cortex are described and the properties of the mitochondria thus prepared are compared with those isolated from the zona fasciculoreticularis. The cristal membranes of mitochondria in the zona glomerulosa in situ are tubular or tubulovesicular, whereas those of mitochondria in the zona fasciculoreticularis in situ are vesicular. When mitochondria are isolated from the former zone, they invariably showed the condensed configuration regardless of isolation media, whereas those isolated from the latter zone in an ST medium showed the orthodox configuration. When Ca2+ was added to mitochondria isolated either from the zona glomerulosa or the zona fasciculoreticularis in an STE medium in the condensed configuration, a transition from the condensed to the orthodox configuration tool place; the cristal membranes of mitochondria from the zona glomerulosa became tubular or tubulovesicular and those of mitochondria from the zona fasciculoreticularis became vesicular. Contaminations of mitrochondria of the zona glomerulosa with other cellular organelles were examined using various marker enzymes. There was no difference in cytochrome content between mitochondria of the two zones specified above. The coupling efficiency of mitochondria of the zona glomerulosa was found to be remarkably effected by temperature during the isolation procedures. Effects of various substrates, isolation media, and bovine serum albumin on the coupling efficiency of mitochondria of the zona glomerulosa are also described.", "PMID": 956163}
{"id": "pubmed23n0032_2031", "title": "Mechanism of activation of bovine factor VII. Products of cleavage by factor Xa.", "content": "Coagulation Factor VII from bovine plasma is a glycoprotein containing a single peptide chain. The NH2-terminal sequence of Ala-Asx-Gly-Phe-Leu- is homologous with the NH2 termini of prothrombin, Factor IX, and the light chain of Factor X. Factor Xa in the presence of calcium ions and phospholipid cleaves Factor VII at an Arg-Ile bond in the sequence Arg-Ile-Val-Gly-Gly-, producing a two-chain molecule with at least 85 times the coagulant activity of single-chain Factor VII and a new NH2-terminal sequence homologous with the corresponding chains of thrombin, Factor IXa and Factor Xa. A second slower cleavage at an Arg-Gly bond destroys Factor VII activity. Bovine Factor VII, unlike prothrombin, Factor IX, and Factor X, is rapidly inhibited by diisopropylphosphorofluoridate (iPr2PF). [3H]iPr2PF is readily incorporated into one-chain, two-chain, and three-chain forms of Factor VII up to ratios of approximately 0.9 moles of [3H]diisopropylphosphate per mole of protein. The radioactive peptides generated from each form of [32P]iPr2PF-inhibited Factor VII by tryptic and thermolytic digestion were found to migrate together on paper electrophoresis. This indicates that the iPr2PF is incorporated stoichiometrically into the same specific site in each form.", "contents": "Mechanism of activation of bovine factor VII. Products of cleavage by factor Xa. Coagulation Factor VII from bovine plasma is a glycoprotein containing a single peptide chain. The NH2-terminal sequence of Ala-Asx-Gly-Phe-Leu- is homologous with the NH2 termini of prothrombin, Factor IX, and the light chain of Factor X. Factor Xa in the presence of calcium ions and phospholipid cleaves Factor VII at an Arg-Ile bond in the sequence Arg-Ile-Val-Gly-Gly-, producing a two-chain molecule with at least 85 times the coagulant activity of single-chain Factor VII and a new NH2-terminal sequence homologous with the corresponding chains of thrombin, Factor IXa and Factor Xa. A second slower cleavage at an Arg-Gly bond destroys Factor VII activity. Bovine Factor VII, unlike prothrombin, Factor IX, and Factor X, is rapidly inhibited by diisopropylphosphorofluoridate (iPr2PF). [3H]iPr2PF is readily incorporated into one-chain, two-chain, and three-chain forms of Factor VII up to ratios of approximately 0.9 moles of [3H]diisopropylphosphate per mole of protein. The radioactive peptides generated from each form of [32P]iPr2PF-inhibited Factor VII by tryptic and thermolytic digestion were found to migrate together on paper electrophoresis. This indicates that the iPr2PF is incorporated stoichiometrically into the same specific site in each form.", "PMID": 956165}
{"id": "pubmed23n0032_2032", "title": "Effect of estradiol on rat uterus DNA-dependent RNA polymerases. Studies on solubilized enzymes.", "content": "DNA-dependent RNA polymerases of immature and castrated rat uteri were studied after estradiol administration. The enzymes were solubilized from either whole uterus homogenate or nuclei and their activities were measured on an exogenous DNA template. alpha-Amanitin was used to distinguish alpha-amanitin-resistant from alpha-amanitin-senitive forms of the enzyme. The number of alpha-amanitin-sensitive RNA polymerase molecules was measured by a binding assay using labeled amanitin. In the first series of experiments RNA polymerases were solubilized from whole uterus homogenate. alpha-Amanitin-sensitive and -resistant activities were constant during the first 6 hours after estradiol treatment, followed by a late and moderate increase in their activities (50% at 12 hours for the resistant form and 40% at 24 hours for the sensitive form). The number of sensitive polymerase molecules evolved in an identical manner to its activity (+40% at 24 hours), suggesting that the increase in activity is due to the synthesis of new enzyme molecules. For both forms, no diffusible stimulatory factor was detected in the uterus of hormone-treated animals. In the second series of experiments, disrupted nuclei were washed with 0.15 M (NH4)2SO4 in order to release only enzyme molecules which were not firmly bound to DNA in a transcription complex. The amount of the sensitive form of polymerase which remains firmly bound to chromatin, was constant for 6 hours after estradiol administration and was doubled by 24 hours. The firmly bound alpha-amanitin-resistant activity was solubilized and was measured in the presence of an exogenous template. There was a progressive increase in activity first detectable in 1 to 2 hours, amounting to 50% at 6 hours and 100% at 24 hours. The reported results show that during the first 6 hours of hormone treatment: (a) the total content of RNA polymerases remains unchanged in the uterus; (b) the number of alpha-amanitin resistant molecules tightly bound to DNA increases progressively while the alpha-amanitin sensitive remains constant. At a later time (24 hours), an increase is observed both for the total amount of enzymes and for their fraction engaged in a transcription complex.", "contents": "Effect of estradiol on rat uterus DNA-dependent RNA polymerases. Studies on solubilized enzymes. DNA-dependent RNA polymerases of immature and castrated rat uteri were studied after estradiol administration. The enzymes were solubilized from either whole uterus homogenate or nuclei and their activities were measured on an exogenous DNA template. alpha-Amanitin was used to distinguish alpha-amanitin-resistant from alpha-amanitin-senitive forms of the enzyme. The number of alpha-amanitin-sensitive RNA polymerase molecules was measured by a binding assay using labeled amanitin. In the first series of experiments RNA polymerases were solubilized from whole uterus homogenate. alpha-Amanitin-sensitive and -resistant activities were constant during the first 6 hours after estradiol treatment, followed by a late and moderate increase in their activities (50% at 12 hours for the resistant form and 40% at 24 hours for the sensitive form). The number of sensitive polymerase molecules evolved in an identical manner to its activity (+40% at 24 hours), suggesting that the increase in activity is due to the synthesis of new enzyme molecules. For both forms, no diffusible stimulatory factor was detected in the uterus of hormone-treated animals. In the second series of experiments, disrupted nuclei were washed with 0.15 M (NH4)2SO4 in order to release only enzyme molecules which were not firmly bound to DNA in a transcription complex. The amount of the sensitive form of polymerase which remains firmly bound to chromatin, was constant for 6 hours after estradiol administration and was doubled by 24 hours. The firmly bound alpha-amanitin-resistant activity was solubilized and was measured in the presence of an exogenous template. There was a progressive increase in activity first detectable in 1 to 2 hours, amounting to 50% at 6 hours and 100% at 24 hours. The reported results show that during the first 6 hours of hormone treatment: (a) the total content of RNA polymerases remains unchanged in the uterus; (b) the number of alpha-amanitin resistant molecules tightly bound to DNA increases progressively while the alpha-amanitin sensitive remains constant. At a later time (24 hours), an increase is observed both for the total amount of enzymes and for their fraction engaged in a transcription complex.", "PMID": 956166}
{"id": "pubmed23n0032_2033", "title": "Free ribosomes in physiologically nondividing cells. Human peripheral lymphocytes.", "content": "Ribosomes of physiologically nondividing human peripheral blood lymphocytes were studied by ultraviolet absorbance measurements of cytoplasmic extracts subjected to ultracentrifugation in sucrose gradients at high and low ionic strength. At least 70% of the total cytoplasmic ribosomes were free ribosomes which sedimented at 80 S in low salt and dissociated to 40 S and 60 S subunits in high salt. These particles were not involved in protein synthesis. The remaining ribosomes were equally divided among native subunits, active monosomes, and larger polysomes. Free ribosomes were shown to exist as 80 S particles in the intact cell, and labeling studies indicated that they did not freely return to the pools of protein-synthesizing components. New ribosomes appeared first as native subunits and in polysomes. After a lag of 15 to 20 min, the particles began to enter the free ribosome pool. Thus, free ribosomes arise in the resting cell by a unidirectional flow which continuously removes particles from the protein-synthesizing pool and sequesters them as an accumulation of inactive 80 S particles. The transition from native subunits to free ribosomes is accompanied by functional changes in association behavior of subunits and by alteration of sedimentation behavior of the subunits. These changes may be due to absence of a protein or proteins from the free ribosomes which is required to permit effective dissociation of subunits prior to initiation of translation. Deficiency of this dissociation factor may be responsible for the continuous formation of free ribosomes in resting cells. Our data also suggest a limitation of the rate of initiation of protein synthesis which may result from deficiency of an initiation factor.", "contents": "Free ribosomes in physiologically nondividing cells. Human peripheral lymphocytes. Ribosomes of physiologically nondividing human peripheral blood lymphocytes were studied by ultraviolet absorbance measurements of cytoplasmic extracts subjected to ultracentrifugation in sucrose gradients at high and low ionic strength. At least 70% of the total cytoplasmic ribosomes were free ribosomes which sedimented at 80 S in low salt and dissociated to 40 S and 60 S subunits in high salt. These particles were not involved in protein synthesis. The remaining ribosomes were equally divided among native subunits, active monosomes, and larger polysomes. Free ribosomes were shown to exist as 80 S particles in the intact cell, and labeling studies indicated that they did not freely return to the pools of protein-synthesizing components. New ribosomes appeared first as native subunits and in polysomes. After a lag of 15 to 20 min, the particles began to enter the free ribosome pool. Thus, free ribosomes arise in the resting cell by a unidirectional flow which continuously removes particles from the protein-synthesizing pool and sequesters them as an accumulation of inactive 80 S particles. The transition from native subunits to free ribosomes is accompanied by functional changes in association behavior of subunits and by alteration of sedimentation behavior of the subunits. These changes may be due to absence of a protein or proteins from the free ribosomes which is required to permit effective dissociation of subunits prior to initiation of translation. Deficiency of this dissociation factor may be responsible for the continuous formation of free ribosomes in resting cells. Our data also suggest a limitation of the rate of initiation of protein synthesis which may result from deficiency of an initiation factor.", "PMID": 956167}
{"id": "pubmed23n0032_2034", "title": "Characteristics of acetaldehyde oxidation in rat liver mitochondria.", "content": "Rat liver mitochondria oxidized acetaldehyde (180 muM) at the rate of approximately 12 nmol/min/mg of protein at 37 degrees. This was stimulated by 88% with the addition of ADP. The ADP/O ratio (2.6) was similar to that with glutamate as substrate. 2,4-Dinitrophenol and phenazine methosulfate also stimulated the rate of acetaldehyde oxidation in the mitochondria. By contrast, acetaldehyde metabolism was virtually abolished by rotenone and antimycin A. These results indicate that acetaldehyde oxidation is linked to the mitochondrial respiratory chain and coupled with mitochondrial oxidative phosphorylation. Indeed, little acetaldehyde was metabolized when mitochondrial membranes were disrupted by sodium deoxycholate. In the disrupted mitochondria, however, acetaldehyde oxidation was fully recovered by addition of NAD+, suggesting that the ability of mitochondria to supply NAD+ controls the rate of acetaldehyde oxidation in intact mitochondria. The stimulatory effect of ADP on mitochondrial acetaldehyde oxidation was diminished by increasing the acetaldehyde concentration. Concomitantly, the ADP/O ratio decreased, suggesting an inhibitory effect of high concentrations of acetaldehyde on mitochondrial respiration. Chronic feeding of ethanol significantly reduced the capacity of intact liver mitochondria to oxidize acetaldehyde. This was associated with a significant reduction of the mitochondrial respiration. By contrast, the activity of aldehyde dehydrogenase in disrupted mitochondria remained unchanged.", "contents": "Characteristics of acetaldehyde oxidation in rat liver mitochondria. Rat liver mitochondria oxidized acetaldehyde (180 muM) at the rate of approximately 12 nmol/min/mg of protein at 37 degrees. This was stimulated by 88% with the addition of ADP. The ADP/O ratio (2.6) was similar to that with glutamate as substrate. 2,4-Dinitrophenol and phenazine methosulfate also stimulated the rate of acetaldehyde oxidation in the mitochondria. By contrast, acetaldehyde metabolism was virtually abolished by rotenone and antimycin A. These results indicate that acetaldehyde oxidation is linked to the mitochondrial respiratory chain and coupled with mitochondrial oxidative phosphorylation. Indeed, little acetaldehyde was metabolized when mitochondrial membranes were disrupted by sodium deoxycholate. In the disrupted mitochondria, however, acetaldehyde oxidation was fully recovered by addition of NAD+, suggesting that the ability of mitochondria to supply NAD+ controls the rate of acetaldehyde oxidation in intact mitochondria. The stimulatory effect of ADP on mitochondrial acetaldehyde oxidation was diminished by increasing the acetaldehyde concentration. Concomitantly, the ADP/O ratio decreased, suggesting an inhibitory effect of high concentrations of acetaldehyde on mitochondrial respiration. Chronic feeding of ethanol significantly reduced the capacity of intact liver mitochondria to oxidize acetaldehyde. This was associated with a significant reduction of the mitochondrial respiration. By contrast, the activity of aldehyde dehydrogenase in disrupted mitochondria remained unchanged.", "PMID": 956168}
{"id": "pubmed23n0032_2035", "title": "Stereospecific, high affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by hepatic cytosol. Evidence that the binding species is receptor for induction of aryl hydrocarbon hydroxylase.", "content": "We previously hypothesized that the genetic trait of aromatic hydrocarbon nonresponsiveness (the failure in certain inbred strains of mice of polycyclic hydrocarbons to induce aryl hydrocarbon hydroxylase activity, and the diminished sensitivity to the more potent inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is due to mutation which results in an induction receptor with a diminished affinity for the inducing compound. Following the intraperitoneal administration of [14C]TCDD (6 nmol/kg), hepatic accumulation of the radiolabel was greatest in C57BL/6J mice, intermediate in the hybrid B6D2F1/J mice, and least in DBA/2J mice, a pattern which mirrors the strain sensitivity to hydroxylase induction by TCDD (C57BL/6J greater than B6D2F1/J greater than DBA/2J). These data are compatible with receptor mutation theory and suggested that the hepatic uptake of TCDD is determined by the affinity of the receptor. In vitro experiments on the binding of [3H]TCDD to hepatic cytosol from C57BL/6J mice revealed a small pool of high affinity sites which stereospecifically and reversibly bind TCDD. The specific binding of [3H]TCDD to hepatic cytosol had an equilibrium dissociation constant KD of 0.27 nM and a maximum binding capacity of 84 fmol/mg of cytosol protein. Much less high affinity specific binding of [3H]TCDD was observed in hepatic cytosol from DBA/2J mice, but the KD was not estimated because of the limited aqueous solubility of the ligand. The binding affinity of 23 halogenated dibenzo-p-dioxins and dibenzofurans for this hepatic cytosol-binding species closely correlated with the potencies of these compounds as inducers of hepatic aryl hydrocarbon hydroxylase activity. The polycyclic hydrocarbons that induce hepatic hydroxylase activity competed with [3H]TCDD for hepatic cytosol binding, but phenobarbital, pregnenolone-16alpha-carbonitrile, and the steroid hormones had no specific binding. The data suggest that the hepatic cytosol species which binds TCDD is the receptor for the induction of hepatic aryl hydrocarbon hydroxylase activity, and that the mutation in nonresponsive mice results in an altered receptor with a diminished affinity for inducing compounds.", "contents": "Stereospecific, high affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by hepatic cytosol. Evidence that the binding species is receptor for induction of aryl hydrocarbon hydroxylase. We previously hypothesized that the genetic trait of aromatic hydrocarbon nonresponsiveness (the failure in certain inbred strains of mice of polycyclic hydrocarbons to induce aryl hydrocarbon hydroxylase activity, and the diminished sensitivity to the more potent inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is due to mutation which results in an induction receptor with a diminished affinity for the inducing compound. Following the intraperitoneal administration of [14C]TCDD (6 nmol/kg), hepatic accumulation of the radiolabel was greatest in C57BL/6J mice, intermediate in the hybrid B6D2F1/J mice, and least in DBA/2J mice, a pattern which mirrors the strain sensitivity to hydroxylase induction by TCDD (C57BL/6J greater than B6D2F1/J greater than DBA/2J). These data are compatible with receptor mutation theory and suggested that the hepatic uptake of TCDD is determined by the affinity of the receptor. In vitro experiments on the binding of [3H]TCDD to hepatic cytosol from C57BL/6J mice revealed a small pool of high affinity sites which stereospecifically and reversibly bind TCDD. The specific binding of [3H]TCDD to hepatic cytosol had an equilibrium dissociation constant KD of 0.27 nM and a maximum binding capacity of 84 fmol/mg of cytosol protein. Much less high affinity specific binding of [3H]TCDD was observed in hepatic cytosol from DBA/2J mice, but the KD was not estimated because of the limited aqueous solubility of the ligand. The binding affinity of 23 halogenated dibenzo-p-dioxins and dibenzofurans for this hepatic cytosol-binding species closely correlated with the potencies of these compounds as inducers of hepatic aryl hydrocarbon hydroxylase activity. The polycyclic hydrocarbons that induce hepatic hydroxylase activity competed with [3H]TCDD for hepatic cytosol binding, but phenobarbital, pregnenolone-16alpha-carbonitrile, and the steroid hormones had no specific binding. The data suggest that the hepatic cytosol species which binds TCDD is the receptor for the induction of hepatic aryl hydrocarbon hydroxylase activity, and that the mutation in nonresponsive mice results in an altered receptor with a diminished affinity for inducing compounds.", "PMID": 956169}
{"id": "pubmed23n0032_2036", "title": "Conformational relevance of the beta6Glu replaced by Val mutation in the beta subunits and in the beta(1-55) and beta(1-30) peptides of hemoglobin S.", "content": "The beta subunits of hemoglobin S showed a higher ellipticity than the beta subunits of the hemoglobin A, both in the Soret and near-ultraviolet regions. The apoderivatives of the beta subunits of hemoglobin S showed a lower helical content and a larger amount of beta conformation than the apoderivatives of the beta subunits of hemoglobin A. The beta(1-55) peptides and beta(1-30) peptides from the beta subunits of hemoglobin A and S have been separated and analyzed. The betaS(1-55) peptide showed a higher content of beta conformation and lower amount of alpha helix when compared to the betaA(1-55) peptide. This difference was present also in different concentrations of methanol. The apoderivative from the beta subunits of hemoglobin S and the betaS(1-55) peptide aggregated with increasing ionic strength. The CD measurement showed that their secondary structure did not change upon a 10-fold dilution of the sample. Very little secondary structure was present in the betaS(1-30) peptide, and the CD spectrum was very similar to that of the betaA(1-30) peptide. No significant difference in aggregation was found between the betaS(1-30) and betaA(1-30) peptides.", "contents": "Conformational relevance of the beta6Glu replaced by Val mutation in the beta subunits and in the beta(1-55) and beta(1-30) peptides of hemoglobin S. The beta subunits of hemoglobin S showed a higher ellipticity than the beta subunits of the hemoglobin A, both in the Soret and near-ultraviolet regions. The apoderivatives of the beta subunits of hemoglobin S showed a lower helical content and a larger amount of beta conformation than the apoderivatives of the beta subunits of hemoglobin A. The beta(1-55) peptides and beta(1-30) peptides from the beta subunits of hemoglobin A and S have been separated and analyzed. The betaS(1-55) peptide showed a higher content of beta conformation and lower amount of alpha helix when compared to the betaA(1-55) peptide. This difference was present also in different concentrations of methanol. The apoderivative from the beta subunits of hemoglobin S and the betaS(1-55) peptide aggregated with increasing ionic strength. The CD measurement showed that their secondary structure did not change upon a 10-fold dilution of the sample. Very little secondary structure was present in the betaS(1-30) peptide, and the CD spectrum was very similar to that of the betaA(1-30) peptide. No significant difference in aggregation was found between the betaS(1-30) and betaA(1-30) peptides.", "PMID": 956170}
{"id": "pubmed23n0032_2037", "title": "Lipid peroxidation and alteration of membrane lipids in isolated hepatocytes exposed to carbon tetrachloride.", "content": "Lipid peroxidation, determined by malondialdehyde formation, occurs at a low, but detectable, rate in parenchymal cells isolated from livers of fasted rats. Pretreatment of rats with phenobarbital increased malondialdehyde formation about 2-fold, probably because of the increased amount of endoplasmic reticulum. Lipid peroxidation was increased in the cells by the addition of either NADPH or CCl4, and the effect of the two agents together was more than additive. Phenobarbital pretreatment increased peroxidation due to exposure of the cells to CCl4 but not that associated with NADPH addition. The amount of CCl4 producing a 50% increase in malondialdehyde formation was about 3-fold less for cells from phenobarbital-treated rats than for those from control rats. Decreased cytochrome P450 levels in isolated hepatocytes produced by prior treatment of rats with allylisopropylacetamide enhanced lipid peroxidation from endogenous substrates but did not affect lipid peroxidation caused by the addition of NADPH. Allylisopropylacetamide markedly lowered lipid peroxidation caused by CCl4 in proportion to the loss of cytochrome P450. Malondialdehyde production associated with metabolism of endogenous substrates in the cells, or resulting from incubation of the cells with NADPH was not accompanied by changes in the fatty acid or protein content of three membranous fractions (microsomal mitochondrial, and cell debris) isolated from homogenates of these cells. Exposure of liver cells to CCl4, however, caused major losses in all fatty acids and of protein from the microsomal fraction, but only polyunsaturated fatty acids were decreased in the cellular debris fraction. Incubation with NADPH and CCl4 together enhanced malondialdehyde formation, but caused no further decrease in fatty acid content in these two fractions. Mitochondrial fatty acids were not decreased by any treatments described.", "contents": "Lipid peroxidation and alteration of membrane lipids in isolated hepatocytes exposed to carbon tetrachloride. Lipid peroxidation, determined by malondialdehyde formation, occurs at a low, but detectable, rate in parenchymal cells isolated from livers of fasted rats. Pretreatment of rats with phenobarbital increased malondialdehyde formation about 2-fold, probably because of the increased amount of endoplasmic reticulum. Lipid peroxidation was increased in the cells by the addition of either NADPH or CCl4, and the effect of the two agents together was more than additive. Phenobarbital pretreatment increased peroxidation due to exposure of the cells to CCl4 but not that associated with NADPH addition. The amount of CCl4 producing a 50% increase in malondialdehyde formation was about 3-fold less for cells from phenobarbital-treated rats than for those from control rats. Decreased cytochrome P450 levels in isolated hepatocytes produced by prior treatment of rats with allylisopropylacetamide enhanced lipid peroxidation from endogenous substrates but did not affect lipid peroxidation caused by the addition of NADPH. Allylisopropylacetamide markedly lowered lipid peroxidation caused by CCl4 in proportion to the loss of cytochrome P450. Malondialdehyde production associated with metabolism of endogenous substrates in the cells, or resulting from incubation of the cells with NADPH was not accompanied by changes in the fatty acid or protein content of three membranous fractions (microsomal mitochondrial, and cell debris) isolated from homogenates of these cells. Exposure of liver cells to CCl4, however, caused major losses in all fatty acids and of protein from the microsomal fraction, but only polyunsaturated fatty acids were decreased in the cellular debris fraction. Incubation with NADPH and CCl4 together enhanced malondialdehyde formation, but caused no further decrease in fatty acid content in these two fractions. Mitochondrial fatty acids were not decreased by any treatments described.", "PMID": 956171}
{"id": "pubmed23n0032_2038", "title": "Delta-Aminolevulinic acid synthetase in the heart.", "content": "The regultion of cardiac delta-aminolevulinic acid synthetase activity was studied in rat heart homogenates. Optimal conditions were determined for the measurement of delta-aminolevulinic acid and an appropriate assay was established for the heart. The activity of cardiac delta-aminolevulinic acid synthetase was determined in rats either fed ad libitum or starved for 24 or 48 h. Marked decreases in delta-aminolevulinic acid synthetase activity were observed in homogenates or mitochondrial fractions prepared from hearts of fasted animals and an explanation for previous findings that the enzyme is undetectable in heart tissue is provided. Dexamethasone treatment was effective in reversing the decreases brought about by fasting but had no effect on the delta-aminolevulinic acid synthetase activity in heart homogenates from fed rats. ACTH treatment had no effect in fed or starved rats. Decreases in delta-aminolevulinic acid synthetase activity induced by fasting were not reversed in homogenates or mitochondrial preparations by succinyl-CoA-generating systems or when alpha-ketoglutarate was substituted for succinate in homogenate preparations. Cardiac delta-aminolevulinic acid dehydratase levels are not altered by fasting. Agents such as allylisopropylacetamide or 3,5-dicarbethoxy-1,4-dihydrocollidine, which produce marked increases in hepatic delta-aminolevulinic acid synthetase activity, have no effects on the activity of this enzyme in the heart.", "contents": "Delta-Aminolevulinic acid synthetase in the heart. The regultion of cardiac delta-aminolevulinic acid synthetase activity was studied in rat heart homogenates. Optimal conditions were determined for the measurement of delta-aminolevulinic acid and an appropriate assay was established for the heart. The activity of cardiac delta-aminolevulinic acid synthetase was determined in rats either fed ad libitum or starved for 24 or 48 h. Marked decreases in delta-aminolevulinic acid synthetase activity were observed in homogenates or mitochondrial fractions prepared from hearts of fasted animals and an explanation for previous findings that the enzyme is undetectable in heart tissue is provided. Dexamethasone treatment was effective in reversing the decreases brought about by fasting but had no effect on the delta-aminolevulinic acid synthetase activity in heart homogenates from fed rats. ACTH treatment had no effect in fed or starved rats. Decreases in delta-aminolevulinic acid synthetase activity induced by fasting were not reversed in homogenates or mitochondrial preparations by succinyl-CoA-generating systems or when alpha-ketoglutarate was substituted for succinate in homogenate preparations. Cardiac delta-aminolevulinic acid dehydratase levels are not altered by fasting. Agents such as allylisopropylacetamide or 3,5-dicarbethoxy-1,4-dihydrocollidine, which produce marked increases in hepatic delta-aminolevulinic acid synthetase activity, have no effects on the activity of this enzyme in the heart.", "PMID": 956172}
{"id": "pubmed23n0032_2039", "title": "Effect of delta9-tetrahydrocannabinol on mitochondrial NADH-oxidase activity.", "content": "Delta9-Tetrahydrocannabinol (delta9-THC), the active ingredient of marihuana was found to be a highly effective inhibitor in vitro of the NADH-oxidase activity of rat brain and heart mitochondria. The degree of inhibition of the enzyme system obtained from rat brain tissue varied with the region from which it was derived as follows, in the presence of 10(-5) M delta9-THC: hypothalamus plus thalamus plus midbrain, 73 +/- 4%; cerebellum, 66 +/- 4%; medulla oblongata plus pons, 63 +/- 6%; cerebral cortex, 50 +/- 8%. The same concentration inhibited rat heart NADH-oxidase activity 69 +/- 9%. Inhibition of NADH-oxidase activity by a corresponding concentration of deoxycorticosterone was significantly less in all tissue preparations tested, ranging from 11% to 26%. The inhibition of delta9-THC appeared to be competitive and near the amytal-sensitive site of the electron transport system. Suggestive evidence was also obtained for a second site of action, above the cytochrome c site.", "contents": "Effect of delta9-tetrahydrocannabinol on mitochondrial NADH-oxidase activity. Delta9-Tetrahydrocannabinol (delta9-THC), the active ingredient of marihuana was found to be a highly effective inhibitor in vitro of the NADH-oxidase activity of rat brain and heart mitochondria. The degree of inhibition of the enzyme system obtained from rat brain tissue varied with the region from which it was derived as follows, in the presence of 10(-5) M delta9-THC: hypothalamus plus thalamus plus midbrain, 73 +/- 4%; cerebellum, 66 +/- 4%; medulla oblongata plus pons, 63 +/- 6%; cerebral cortex, 50 +/- 8%. The same concentration inhibited rat heart NADH-oxidase activity 69 +/- 9%. Inhibition of NADH-oxidase activity by a corresponding concentration of deoxycorticosterone was significantly less in all tissue preparations tested, ranging from 11% to 26%. The inhibition of delta9-THC appeared to be competitive and near the amytal-sensitive site of the electron transport system. Suggestive evidence was also obtained for a second site of action, above the cytochrome c site.", "PMID": 956173}
{"id": "pubmed23n0032_2040", "title": "Negative cooperativity among beta-adrenergic receptors in frog erythrocyte membranes.", "content": "(-)-[3H]Dihydroalprenolol, a potent competitive beta-adrenergic antagonist, has been previously documented to bind to the adenylate cyclase-coupled beta-adrenergic receptor sites in mammalian and non-mammalian tissues. Steady state binding of (-)-[3H]dihydroalprenolol to sites in frog erythrocyte membranes, a model system for adenylate cyclase-coupled beta-adrenergic receptors, displays characteristics consistent with negative cooperativity among the beta-adrenergic receptors: Scatchard plots are curvilinear with upward concavity and slopes of Hill plots are consistently less than 1.0. The existence of site-site interactions of the negatively cooperative type were demonstrated directly by the ability of unlabeled (-)-alprenolol to accelerate the dissociation of (-)-[3H]dihydroalprenolol under conditions were no rebinding of radioligand occurred. The dissociation rate of (-)-[3H]dihydroalprenolol alone is directly related to temperature and increases with increases in temperature from 4-37 degrees. (-)-[3H]Dihydroalprenolol dissociation is enhanced by unlabeled (-)-alprenolol at all temperatures studied; however, at 4 degrees, the time required to observe an enhancement of radioligand dissociated is greater than the time required for unlabeled (-)-alprenolol to occupy the empty receptor sites, suggesting that increased rigidity of the biomembrane at 4 degrees may be responsible for the absence of readily observable site-site interactions. The ability of a number of beta-adrenergic agonists and antagonists to induce negative cooperativity among the beta-adrenergic receptors was directly related to their affinity for the receptor sites rather than their intrinsic activity in the adenylate cyclase-coupled beta-adrenergic system. The ability to induce site-site interactions among the beta-adrenergic receptors occurs at physiological concentrations of beta-adrenergic agents, since occupancy of less than 10% of the receptor sites is sufficient to reduce receptor affinity. Changes in pH from 6.5 to 9.0 did not significantly alter the negatively cooperative site-site interactions among the receptor sites. The negatively cooperative phenomenon was also independent of Mg2+, Ca2+, and NaF concentrations in the buffer medium. The presence of guanyl-5'-yl imidodiphosphate, a nonhydrolyzable nucleotide analog which enhances adenylate cyclase stimulation (Vmax) by beta-adrenergic agonists and decreases the concentration of agonist required to half-maximally stimulate adenylate cyclase, did not alter the ability of either agonists or antagonists to induce negatively cooperative site-site interactions among the beta-adrenergic receptors.", "contents": "Negative cooperativity among beta-adrenergic receptors in frog erythrocyte membranes. (-)-[3H]Dihydroalprenolol, a potent competitive beta-adrenergic antagonist, has been previously documented to bind to the adenylate cyclase-coupled beta-adrenergic receptor sites in mammalian and non-mammalian tissues. Steady state binding of (-)-[3H]dihydroalprenolol to sites in frog erythrocyte membranes, a model system for adenylate cyclase-coupled beta-adrenergic receptors, displays characteristics consistent with negative cooperativity among the beta-adrenergic receptors: Scatchard plots are curvilinear with upward concavity and slopes of Hill plots are consistently less than 1.0. The existence of site-site interactions of the negatively cooperative type were demonstrated directly by the ability of unlabeled (-)-alprenolol to accelerate the dissociation of (-)-[3H]dihydroalprenolol under conditions were no rebinding of radioligand occurred. The dissociation rate of (-)-[3H]dihydroalprenolol alone is directly related to temperature and increases with increases in temperature from 4-37 degrees. (-)-[3H]Dihydroalprenolol dissociation is enhanced by unlabeled (-)-alprenolol at all temperatures studied; however, at 4 degrees, the time required to observe an enhancement of radioligand dissociated is greater than the time required for unlabeled (-)-alprenolol to occupy the empty receptor sites, suggesting that increased rigidity of the biomembrane at 4 degrees may be responsible for the absence of readily observable site-site interactions. The ability of a number of beta-adrenergic agonists and antagonists to induce negative cooperativity among the beta-adrenergic receptors was directly related to their affinity for the receptor sites rather than their intrinsic activity in the adenylate cyclase-coupled beta-adrenergic system. The ability to induce site-site interactions among the beta-adrenergic receptors occurs at physiological concentrations of beta-adrenergic agents, since occupancy of less than 10% of the receptor sites is sufficient to reduce receptor affinity. Changes in pH from 6.5 to 9.0 did not significantly alter the negatively cooperative site-site interactions among the receptor sites. The negatively cooperative phenomenon was also independent of Mg2+, Ca2+, and NaF concentrations in the buffer medium. The presence of guanyl-5'-yl imidodiphosphate, a nonhydrolyzable nucleotide analog which enhances adenylate cyclase stimulation (Vmax) by beta-adrenergic agonists and decreases the concentration of agonist required to half-maximally stimulate adenylate cyclase, did not alter the ability of either agonists or antagonists to induce negatively cooperative site-site interactions among the beta-adrenergic receptors.", "PMID": 956174}
{"id": "pubmed23n0032_2041", "title": "Isolation and characterization of subcellular membranes with altered phospholipid composition from cultured fibroblasts.", "content": "Plasma membranes, microsomes, and mitochondria were isolated from mouse fibroblast (LM) suspension cells by modification of several established procedures. Choline analogues such as N,N'-dimethylethanolamine, N-monomethylethanolamine, or ethanolamine were incorporated in vivo into phospholipids of all three cell fractions studied, but to varying degrees depending on the type of analogue used. The in vivo incorporation of these bases into membrane phospholipids produced no significant effect on the activities of seven membrane-bound enzymes: (Na+, K+)-ATPase, 5'-nucleotidase (plasma membranes); TPNH-cytochrome c reductase, glucose-6-phosphatase, inosine diphosphatase (microsomes); and succinate cytochrome c reductase (mitochondria). The incorporation of base analogues into phospholipids was accompanied by several compensatory mechanisms. (a) The quantity of both phosphatidylcholine and phosphatidylethanolamine decreased up to 75% and 50% respectively in 3 days. (b) The molar ratio of desmosterol/phospholipid in the plasma membranes of LM cells grown in suspension culture in the presence of choline analogues decreased from 0.65 to 0.45. (c) The percentage of lysophosphatidylcholine increased over 2-fold in the phospholipid of all subcellular fractions studied. The quantity of lysophosphatidylcholine was directly proportional to the number of methyl groups on the nitrogen atom of the base analogue supplemented to the cells. This was a specific effect since the quantity of lysophosphatidylethanolamine, the other major lysophospholipid, remained unchanged. (d) The ratio of zwitterionic phospholipids to acidic phospholipids remained relatively constant in all isolated membrane fractions regardless of analogue supplementation. Neither increase in the degree of unsaturation nor shortening of fatty acid chain length was noted in response to analogue supplementation.", "contents": "Isolation and characterization of subcellular membranes with altered phospholipid composition from cultured fibroblasts. Plasma membranes, microsomes, and mitochondria were isolated from mouse fibroblast (LM) suspension cells by modification of several established procedures. Choline analogues such as N,N'-dimethylethanolamine, N-monomethylethanolamine, or ethanolamine were incorporated in vivo into phospholipids of all three cell fractions studied, but to varying degrees depending on the type of analogue used. The in vivo incorporation of these bases into membrane phospholipids produced no significant effect on the activities of seven membrane-bound enzymes: (Na+, K+)-ATPase, 5'-nucleotidase (plasma membranes); TPNH-cytochrome c reductase, glucose-6-phosphatase, inosine diphosphatase (microsomes); and succinate cytochrome c reductase (mitochondria). The incorporation of base analogues into phospholipids was accompanied by several compensatory mechanisms. (a) The quantity of both phosphatidylcholine and phosphatidylethanolamine decreased up to 75% and 50% respectively in 3 days. (b) The molar ratio of desmosterol/phospholipid in the plasma membranes of LM cells grown in suspension culture in the presence of choline analogues decreased from 0.65 to 0.45. (c) The percentage of lysophosphatidylcholine increased over 2-fold in the phospholipid of all subcellular fractions studied. The quantity of lysophosphatidylcholine was directly proportional to the number of methyl groups on the nitrogen atom of the base analogue supplemented to the cells. This was a specific effect since the quantity of lysophosphatidylethanolamine, the other major lysophospholipid, remained unchanged. (d) The ratio of zwitterionic phospholipids to acidic phospholipids remained relatively constant in all isolated membrane fractions regardless of analogue supplementation. Neither increase in the degree of unsaturation nor shortening of fatty acid chain length was noted in response to analogue supplementation.", "PMID": 956175}
{"id": "pubmed23n0032_2042", "title": "Synthesis in vitro of intrinsic membrane proteins by free, membrane-bound, and Golgi apparatus-associated polyribosomes from rat liver.", "content": "A fraction of intrinsic membrane proteins was prepared from the major membranous cell components of rat liver by extraction of the membranes with KCl and deoxycholate. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the compositions of the intrinsic protein fractions from rough and endoplasmic reticulum, smooth endoplasmic reticulum. Golgi apparatus, plasma membrane, and nuclear envelope were similar to each other but distinct from that of mitochondria. Among endomembranes, differences were in the ratios of protein constituents plus a few protein bands of Golgi apparatus and plasma membranes not found in endoplasmic reticulum or nuclear envelope. The abilities of total rough endoplasmic reticulum, polysomes released from rough endoplasmic reticulum, and free polysomes to incorporate amino acids into the intrinsic protein fraction were tested in vitro. Polysomes bound to endoplasmic reticulum has the greatest capacity to synthesize proteins of this fraction as shown by co-purification of radioactive products and by immunoprecipitation. Although the majority of the radioactive products synthesized by bound polysomes were distinct from those synthesized by free polysomes, certain radioactive products synthesized by free polysomes also co-purified with intrinsic membrane proteins. The results show no absolute segregation between free and bound polysomes in the synthesis of intrinsic membrane proteins. However, the majority of these proteins appear to be synthesized by polysomes bound to the endoplasmic reticulum. Several intrinsic proteins found in plasma membranes do not appear in rough endoplasmic reticulum. To determine where these proteins were synthesized, the ability of other endomembrane components to support in vitro incorporation of [14C]leucine into protein was examined. In contrast to plasma membranes, isolated Golgi apparatus fractions did incorporate [14C]leucine to an extent greater than could be explained by contamination with rough endoplasmic reticulum. Golgi apparatus in situ and isolated from rat liver have polyribosomes associated with a zone of cytoplasm at the Golgi apparatus periphery occupied by tubules and vesicles. The polysomes are not directly attached to membranes as with rough endoplasmic reticulum and may represent a special class of \"Golgi apparatus-associated\" polysomes. The polysomes, when associated with Golgi apparatus membranes, incorporated amino acids in vitro. The products synthesized in vitro were analyzed by treatment with KCl and deoxycholate and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Certain proteins synthesized by the Golgi apparatus-associated polysomes remained insoluble after the treatment with KCl and deoxycholate. The proteins synthesized by the Golgi apparatus fraction had mobilities similar to proteins in plasma membranes which were absent from endoplasmic reticulum, and which were relatively minor components of Golgi apparatus...", "contents": "Synthesis in vitro of intrinsic membrane proteins by free, membrane-bound, and Golgi apparatus-associated polyribosomes from rat liver. A fraction of intrinsic membrane proteins was prepared from the major membranous cell components of rat liver by extraction of the membranes with KCl and deoxycholate. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the compositions of the intrinsic protein fractions from rough and endoplasmic reticulum, smooth endoplasmic reticulum. Golgi apparatus, plasma membrane, and nuclear envelope were similar to each other but distinct from that of mitochondria. Among endomembranes, differences were in the ratios of protein constituents plus a few protein bands of Golgi apparatus and plasma membranes not found in endoplasmic reticulum or nuclear envelope. The abilities of total rough endoplasmic reticulum, polysomes released from rough endoplasmic reticulum, and free polysomes to incorporate amino acids into the intrinsic protein fraction were tested in vitro. Polysomes bound to endoplasmic reticulum has the greatest capacity to synthesize proteins of this fraction as shown by co-purification of radioactive products and by immunoprecipitation. Although the majority of the radioactive products synthesized by bound polysomes were distinct from those synthesized by free polysomes, certain radioactive products synthesized by free polysomes also co-purified with intrinsic membrane proteins. The results show no absolute segregation between free and bound polysomes in the synthesis of intrinsic membrane proteins. However, the majority of these proteins appear to be synthesized by polysomes bound to the endoplasmic reticulum. Several intrinsic proteins found in plasma membranes do not appear in rough endoplasmic reticulum. To determine where these proteins were synthesized, the ability of other endomembrane components to support in vitro incorporation of [14C]leucine into protein was examined. In contrast to plasma membranes, isolated Golgi apparatus fractions did incorporate [14C]leucine to an extent greater than could be explained by contamination with rough endoplasmic reticulum. Golgi apparatus in situ and isolated from rat liver have polyribosomes associated with a zone of cytoplasm at the Golgi apparatus periphery occupied by tubules and vesicles. The polysomes are not directly attached to membranes as with rough endoplasmic reticulum and may represent a special class of \"Golgi apparatus-associated\" polysomes. The polysomes, when associated with Golgi apparatus membranes, incorporated amino acids in vitro. The products synthesized in vitro were analyzed by treatment with KCl and deoxycholate and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Certain proteins synthesized by the Golgi apparatus-associated polysomes remained insoluble after the treatment with KCl and deoxycholate. The proteins synthesized by the Golgi apparatus fraction had mobilities similar to proteins in plasma membranes which were absent from endoplasmic reticulum, and which were relatively minor components of Golgi apparatus...", "PMID": 956176}
{"id": "pubmed23n0032_2043", "title": "High uptake of myo-inositol by rat pancreatic tissue in vitro stimulates secretion.", "content": "A recent study by Hokin-Neaverson, M., Sadeghian, K., Majumder, A.L., and Eisenberg, F. (1975) Biochem. Biophys. Res. Commun. 67, 1537-1544, demonstrates that free myo-inositol in the pancreas is significantly increased during intense cholinergic stimulation of secretion. Incubation of rat pancreatic tissue in medium with 100 mM myo-inositol increases 10-fold the endogenous content of free myo-inositol and elicits a prompt and sustained 50% increase in the rate of release of amylase activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that the electrophoretic pattern of the protein mixture released in the presence of 100 mM myo-inositol is the same as that of the secretory output released in the presence of 10 muM carbamylcholine. Microscopic examination of tissue pieces indicates that there is no significant decrease in zymogen granule content of the pancreatic acinar cells during incubation in medium with 100 mM myo-inositol. Jamieson, J.D., and Palade, G.E. (1967) J. Cell Biol. 34, 597-615, have shown that pulse-labeled secretory proteins in guinea pig pancreas first appear in zymogen granules 1 hour postpulse, becoming maximally accumulated in these storage sites by 2 hours postpulse. myo-Inositol (100 mM) stimulates release of pulse-labeled secretory proteins only if incubation in medium with 100 mM myo-inositol is initiated anytime during the first 80 min postpulse. The findings thus indicate that a high uptake of myo-inositol by rat pancreatic tissue in vitro selectively stimulates the release of just those secretory proteins being packaged in newly forming zymogen granules.", "contents": "High uptake of myo-inositol by rat pancreatic tissue in vitro stimulates secretion. A recent study by Hokin-Neaverson, M., Sadeghian, K., Majumder, A.L., and Eisenberg, F. (1975) Biochem. Biophys. Res. Commun. 67, 1537-1544, demonstrates that free myo-inositol in the pancreas is significantly increased during intense cholinergic stimulation of secretion. Incubation of rat pancreatic tissue in medium with 100 mM myo-inositol increases 10-fold the endogenous content of free myo-inositol and elicits a prompt and sustained 50% increase in the rate of release of amylase activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that the electrophoretic pattern of the protein mixture released in the presence of 100 mM myo-inositol is the same as that of the secretory output released in the presence of 10 muM carbamylcholine. Microscopic examination of tissue pieces indicates that there is no significant decrease in zymogen granule content of the pancreatic acinar cells during incubation in medium with 100 mM myo-inositol. Jamieson, J.D., and Palade, G.E. (1967) J. Cell Biol. 34, 597-615, have shown that pulse-labeled secretory proteins in guinea pig pancreas first appear in zymogen granules 1 hour postpulse, becoming maximally accumulated in these storage sites by 2 hours postpulse. myo-Inositol (100 mM) stimulates release of pulse-labeled secretory proteins only if incubation in medium with 100 mM myo-inositol is initiated anytime during the first 80 min postpulse. The findings thus indicate that a high uptake of myo-inositol by rat pancreatic tissue in vitro selectively stimulates the release of just those secretory proteins being packaged in newly forming zymogen granules.", "PMID": 956177}
{"id": "pubmed23n0032_2044", "title": "Formyl-methyl-methylenetetrahydrofolate synthetase-(combined). An ovine protein with multiple catalytic activities.", "content": "Formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase, and methylenetetrahydrofolate dehydrogenase in sheep liver co-purify 200-fold to yield a homogeneous preparation containing a single protein species observed on discontinuous polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. The synthetase and dehydrogenase activities migrate with the single protein band observed on polyacrylamide gel electrophoresis in two discontinuous buffer systems near pH 8. The protein sediments with a single symmetrical boundary with sedimentation coefficient (S20,w) = 8.30 S and elutes from molecular exclusion chromatography columns at a position corresponding to a diffusion coefficient (D20,w) = 3.99 X 10(-7) cm2 sec-1. Dodecyl sulfate-polyacrylamide gel electrophoresis of the protein intramolecularly cross-linked with dimethylsuberimidate shows one protein species of Mr = 218,000 in addition to the species of Mr = 108,500 characteristic of the unmodified protein. NH2-terminal analysis of the protein using 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) chloride in the presence of dodecyl sulfate results in the recovery of a single dansyl amino acid, alanine. The three activities are thus shown to reside in a protein composed of two apparently identical subunits, and the trivial name formyl-methenyl-methylenetetrahydrofolate synthetase(combined) is proposed for this enzyme to suggest the multiple catalytic activities associated with the single protein species.", "contents": "Formyl-methyl-methylenetetrahydrofolate synthetase-(combined). An ovine protein with multiple catalytic activities. Formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase, and methylenetetrahydrofolate dehydrogenase in sheep liver co-purify 200-fold to yield a homogeneous preparation containing a single protein species observed on discontinuous polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. The synthetase and dehydrogenase activities migrate with the single protein band observed on polyacrylamide gel electrophoresis in two discontinuous buffer systems near pH 8. The protein sediments with a single symmetrical boundary with sedimentation coefficient (S20,w) = 8.30 S and elutes from molecular exclusion chromatography columns at a position corresponding to a diffusion coefficient (D20,w) = 3.99 X 10(-7) cm2 sec-1. Dodecyl sulfate-polyacrylamide gel electrophoresis of the protein intramolecularly cross-linked with dimethylsuberimidate shows one protein species of Mr = 218,000 in addition to the species of Mr = 108,500 characteristic of the unmodified protein. NH2-terminal analysis of the protein using 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) chloride in the presence of dodecyl sulfate results in the recovery of a single dansyl amino acid, alanine. The three activities are thus shown to reside in a protein composed of two apparently identical subunits, and the trivial name formyl-methenyl-methylenetetrahydrofolate synthetase(combined) is proposed for this enzyme to suggest the multiple catalytic activities associated with the single protein species.", "PMID": 956178}
{"id": "pubmed23n0032_2045", "title": "Fragmentation of the 95,000-dalton transmembrane polypeptide in human erythrocyte membranes.", "content": "The 95,000-dalton polypeptide in human red blood cell membranes constitutes about 25% of the membrane protein. Previous labeling studies have shown that different regions of this polypeptide are exposed to the inside and outside of the cell and have suggested a role for the protein in anion exchange across the membrane. This polypeptide has been fragmented by chymotrypsin digestion of intact red cells and by treatment of purified polypeptide with 2-nitro-5-thiocyanobenzoic acid, hydroxylamine, and N-bromosuccinimide. The sites of cleavage by each of these reagents have been located relative to the NH-2 and COOH-terminals of the intact 95,000-dalton polypeptide. Polypeptide obtained from cells labeled with 1-isothiocyanate-4-benzene [35S]sulfonic acid (an inhibitor of anion transport), 125I and lactoperoxidase, or 32P has been similarly fragmented and these labels have been assigned to specific regions of the polypeptide. There are at least two sites of phosphorylation of the polypeptide; the major sites lies within 10,000 daltons of the NH2-terminal requiring that this portion of the polypeptide lie inside the cell. Sites of chymotrypsin cleavage and 125I and lactoperoxidase labeling are in a 7,000-dalton region toward the COOH-terminal of the polypeptide; this region must lie outside the cell. Between these two regions the polypeptide must traverse the lipid bilayer an odd number of times. 1-Isothiocyanate-4-benzenesulfonic acid also labels the protein near the site of chymotrypsin cleavage.", "contents": "Fragmentation of the 95,000-dalton transmembrane polypeptide in human erythrocyte membranes. The 95,000-dalton polypeptide in human red blood cell membranes constitutes about 25% of the membrane protein. Previous labeling studies have shown that different regions of this polypeptide are exposed to the inside and outside of the cell and have suggested a role for the protein in anion exchange across the membrane. This polypeptide has been fragmented by chymotrypsin digestion of intact red cells and by treatment of purified polypeptide with 2-nitro-5-thiocyanobenzoic acid, hydroxylamine, and N-bromosuccinimide. The sites of cleavage by each of these reagents have been located relative to the NH-2 and COOH-terminals of the intact 95,000-dalton polypeptide. Polypeptide obtained from cells labeled with 1-isothiocyanate-4-benzene [35S]sulfonic acid (an inhibitor of anion transport), 125I and lactoperoxidase, or 32P has been similarly fragmented and these labels have been assigned to specific regions of the polypeptide. There are at least two sites of phosphorylation of the polypeptide; the major sites lies within 10,000 daltons of the NH2-terminal requiring that this portion of the polypeptide lie inside the cell. Sites of chymotrypsin cleavage and 125I and lactoperoxidase labeling are in a 7,000-dalton region toward the COOH-terminal of the polypeptide; this region must lie outside the cell. Between these two regions the polypeptide must traverse the lipid bilayer an odd number of times. 1-Isothiocyanate-4-benzenesulfonic acid also labels the protein near the site of chymotrypsin cleavage.", "PMID": 956179}
{"id": "pubmed23n0032_2046", "title": "Reversible inhibition of glucose transport in human erythrocytes by a series of pyridine derivatives.", "content": "A series of pyridine derivatives was chosen on the basis of structural similarity to glucose and to the hydrogen bonding capacity of this sugar. These compounds were found to reversibly inhibit glucose transport in a systematic fashion. The inhibition data can be rationalized by assuming that both competitive and noncompetitive binding sites exist on the cell membrane. The competitive site appears to have a much lower dissociation constant than the noncompetitive site for most derivatives. The effects of changes in substituents on the pyridine ring closely match, both in ring position and in the magnitude of the change in binding constant, those reported for derivatives of glucose. The stimulation of fluorodinitrobenzene inactivation of glucose transport by 2-amino-5-chloropyridine is in accord with this analysis. The strongest inhibitors bind about 100-fold more tightly than glucose itself. This series of pyridine compounds would appear to present a useful starting point for the development of site-specific irreversible inhibitors.", "contents": "Reversible inhibition of glucose transport in human erythrocytes by a series of pyridine derivatives. A series of pyridine derivatives was chosen on the basis of structural similarity to glucose and to the hydrogen bonding capacity of this sugar. These compounds were found to reversibly inhibit glucose transport in a systematic fashion. The inhibition data can be rationalized by assuming that both competitive and noncompetitive binding sites exist on the cell membrane. The competitive site appears to have a much lower dissociation constant than the noncompetitive site for most derivatives. The effects of changes in substituents on the pyridine ring closely match, both in ring position and in the magnitude of the change in binding constant, those reported for derivatives of glucose. The stimulation of fluorodinitrobenzene inactivation of glucose transport by 2-amino-5-chloropyridine is in accord with this analysis. The strongest inhibitors bind about 100-fold more tightly than glucose itself. This series of pyridine compounds would appear to present a useful starting point for the development of site-specific irreversible inhibitors.", "PMID": 956180}
{"id": "pubmed23n0032_2047", "title": "Effect of a supernatant protein on microsomal squalene epoxidase and 2,3-oxidosqualene-lanosterol cyclase.", "content": "Squalene epoxidation catalyzed by rat liver microsomes requires oxygen NADPH, and the 105,000 x g supernatant (S105). The supernatant can be replaced by a partially purified S105 protein (SPF) and phospholipids (Tai, H., and Bloch, K. (1972) J. Biol. Chem. 247, 3767). When washed microsomes are preincubated anaerobically with [14C]squalene and S105 without NADPH, followed by centrifugation and washing to remove the unbound squalene and S105, epoxidation in the presence of O2 and NADPH occurs subsequently at the same rate as in direct assays containing all required components from the start. Partially purified SPF (65-fold) shows the same effect. Washed microsomes preincubated anaerobically with squalene alone, or with bovine serum albumin instead of S105, also take up large amounts of squalene, but the squalene so incorporated is only poorly converted to epoxide. The epoxidation of endogenous squalene formed in liver homogenates from [14C]mevalonate is also stimulated by S105. The incorporation of squalene into microsomes is temperature dependent. 2,3-Oxidosqualene-lanosterol cyclase (cyclase) also requires S105 for optimal activity. It is suggested that the S105 protein acts internally within the microsomal membrane system facilitating the access of substrate to specific enzyme sites.", "contents": "Effect of a supernatant protein on microsomal squalene epoxidase and 2,3-oxidosqualene-lanosterol cyclase. Squalene epoxidation catalyzed by rat liver microsomes requires oxygen NADPH, and the 105,000 x g supernatant (S105). The supernatant can be replaced by a partially purified S105 protein (SPF) and phospholipids (Tai, H., and Bloch, K. (1972) J. Biol. Chem. 247, 3767). When washed microsomes are preincubated anaerobically with [14C]squalene and S105 without NADPH, followed by centrifugation and washing to remove the unbound squalene and S105, epoxidation in the presence of O2 and NADPH occurs subsequently at the same rate as in direct assays containing all required components from the start. Partially purified SPF (65-fold) shows the same effect. Washed microsomes preincubated anaerobically with squalene alone, or with bovine serum albumin instead of S105, also take up large amounts of squalene, but the squalene so incorporated is only poorly converted to epoxide. The epoxidation of endogenous squalene formed in liver homogenates from [14C]mevalonate is also stimulated by S105. The incorporation of squalene into microsomes is temperature dependent. 2,3-Oxidosqualene-lanosterol cyclase (cyclase) also requires S105 for optimal activity. It is suggested that the S105 protein acts internally within the microsomal membrane system facilitating the access of substrate to specific enzyme sites.", "PMID": 956181}
{"id": "pubmed23n0032_2048", "title": "Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement.", "content": "The ts CB1200 (antimutator) mutation in bacteriophage T4 DNA polymerase increases the accuracy of DNA replication since it results in a decrease in the frequency of mutations in other phage genes. The CB120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand, even in the presence of the T4 DNA unwinding protein (gene 32-protein). The ratio of nucleotides turned over (DNA-dependent conversion of deoxynucleoside triphosphate to deoxynucleoside monophosphate) to nucleotides stably incorporated into product is 10 to 100 times higher with the mutant than wild type enzyme, depending on the DNA used as the template. This high turnover rate may increase the efficiency of removal of noncomplementary nucleotides by the antimutator enzyme and is in agreement with the findings of Muzyczka et al, (Muzyczka, N., Poland, R. L., and Bessman, M. J. (1972) J. Biol, Cehm. 247, 7116-7122) with the L141 and L42 antimutator T4 DNA polymerases. Since the 3'- to 5'-exonuclease activity of the CB120 mutant polymerase is not higher than that of the wild type enzyme, it is suggested that the high turnover rate may result from increased opportunity to remove newly incorporated nucleotides due to the slow rate at which the mutant enzyme moves to the next template nucleotide. In the accompanying paper we show that the CB120 antimutator polymerase also initially selects incorrect nucleotides for incorporation less frequently than the wild type enzyme. Thus this antimutator polymerase appears to have both greater accuracy in nucleotide selection and an enhanced ability to remove incorrect nucleotides.", "contents": "Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement. The ts CB1200 (antimutator) mutation in bacteriophage T4 DNA polymerase increases the accuracy of DNA replication since it results in a decrease in the frequency of mutations in other phage genes. The CB120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand, even in the presence of the T4 DNA unwinding protein (gene 32-protein). The ratio of nucleotides turned over (DNA-dependent conversion of deoxynucleoside triphosphate to deoxynucleoside monophosphate) to nucleotides stably incorporated into product is 10 to 100 times higher with the mutant than wild type enzyme, depending on the DNA used as the template. This high turnover rate may increase the efficiency of removal of noncomplementary nucleotides by the antimutator enzyme and is in agreement with the findings of Muzyczka et al, (Muzyczka, N., Poland, R. L., and Bessman, M. J. (1972) J. Biol, Cehm. 247, 7116-7122) with the L141 and L42 antimutator T4 DNA polymerases. Since the 3'- to 5'-exonuclease activity of the CB120 mutant polymerase is not higher than that of the wild type enzyme, it is suggested that the high turnover rate may result from increased opportunity to remove newly incorporated nucleotides due to the slow rate at which the mutant enzyme moves to the next template nucleotide. In the accompanying paper we show that the CB120 antimutator polymerase also initially selects incorrect nucleotides for incorporation less frequently than the wild type enzyme. Thus this antimutator polymerase appears to have both greater accuracy in nucleotide selection and an enhanced ability to remove incorrect nucleotides.", "PMID": 956182}
{"id": "pubmed23n0032_2049", "title": "Control of mutation frequency by bacteriophage T4 DNA polymerase. II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases.", "content": "The accuracy of nucleotide selection by wild type, L88 mutator, and CB120 antimutator T4 DNA polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the DNA-dependent conversion of deoxynucleoside triphosphate to monophosphate. The increased accuracy of the CB120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(dA)-poly(dT) as template which is only 10 to 30% of that of the wild type enzyme. In contrast, the ratio of incorrect to correct nucleotide utilized by the L88 mutator enzyme with this template was higher than that of the wild type enzyme, in agreement with the report of Hershfield (Hershfield, M.S. (1973) J. Biol, Chem. 248, 1417-1423). The antimutator, mutator, and wild type enzymes each have a much higher apparent Km for the noncomplementary nucleotides than for complementary nucleotides with this template. The L88 mutator polymerase has a higher \"Km\" for poly(dA)-poly(dT) than the wild type enzyme in reactions with both complementary and noncomplementary nucleotides. The antimutator polymerase has an elevated \"Km\" for polymer only for reactions with noncomplementary nucleotides. The wild type and L88 mutator enzymes also utilized both noncomplementary nucleotides much more frequently than the CB120 antimutator enzymes with poly [d(A-T)] as the template-primer. The T4 gene 32DNA unwinding protein appears to facilitate the correct reading of this template since it decreases the ratio of incorrect nucleotides utilized by both the wild type and L88 mutator polymerases.", "contents": "Control of mutation frequency by bacteriophage T4 DNA polymerase. II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases. The accuracy of nucleotide selection by wild type, L88 mutator, and CB120 antimutator T4 DNA polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the DNA-dependent conversion of deoxynucleoside triphosphate to monophosphate. The increased accuracy of the CB120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(dA)-poly(dT) as template which is only 10 to 30% of that of the wild type enzyme. In contrast, the ratio of incorrect to correct nucleotide utilized by the L88 mutator enzyme with this template was higher than that of the wild type enzyme, in agreement with the report of Hershfield (Hershfield, M.S. (1973) J. Biol, Chem. 248, 1417-1423). The antimutator, mutator, and wild type enzymes each have a much higher apparent Km for the noncomplementary nucleotides than for complementary nucleotides with this template. The L88 mutator polymerase has a higher \"Km\" for poly(dA)-poly(dT) than the wild type enzyme in reactions with both complementary and noncomplementary nucleotides. The antimutator polymerase has an elevated \"Km\" for polymer only for reactions with noncomplementary nucleotides. The wild type and L88 mutator enzymes also utilized both noncomplementary nucleotides much more frequently than the CB120 antimutator enzymes with poly [d(A-T)] as the template-primer. The T4 gene 32DNA unwinding protein appears to facilitate the correct reading of this template since it decreases the ratio of incorrect nucleotides utilized by both the wild type and L88 mutator polymerases.", "PMID": 956183}
{"id": "pubmed23n0032_2050", "title": "Alpha-Hydroxylation of lignoceric and nervonic acids in the brain. Effects of altered thyroid function on postnatal development of the hydroxylase activity.", "content": "Rat brain postnuclear preparations catalyzed the alpha-hydroxylation of nervonic acid with an apparent Km of 3 muM. Evidence has been presented which suggests that nervonic acid in the brain is hydroxylated by the same enzyme system which hydroxylates lignoceric acid. The hydroxylase activity in brains of normal (euthyroid) rats increased rapidly from a low in the period immediately following birth to a maximum at the 23rd day and then declined to a low level characteristic of the mature brain. Neonatal hypothyroidism retarded the development of the activity and shifted its peak to the 39th day after birth. Conversely, neonatal hyperthyroidism accelerated the entire developmental pattern and shifted the peak to the 16th day after birth. The hydroxylase activity in mouse brain was also increased by thyroid hormone administration from the 13th through the 18th day after birth. Unlike normal mice, the low activity in jimpy mice was not affected by this treatment. It is concluded that thyroid hormones play an important role in the control of brain fatty acid alpha-hydroxylation. The stimulation of alpha-hydroxy fatty acid synthesis in response to hyperthyroidism during the early postnatal period may be one of the major effects of thyroid hormones in accelerating myelination of the central nervous system.", "contents": "Alpha-Hydroxylation of lignoceric and nervonic acids in the brain. Effects of altered thyroid function on postnatal development of the hydroxylase activity. Rat brain postnuclear preparations catalyzed the alpha-hydroxylation of nervonic acid with an apparent Km of 3 muM. Evidence has been presented which suggests that nervonic acid in the brain is hydroxylated by the same enzyme system which hydroxylates lignoceric acid. The hydroxylase activity in brains of normal (euthyroid) rats increased rapidly from a low in the period immediately following birth to a maximum at the 23rd day and then declined to a low level characteristic of the mature brain. Neonatal hypothyroidism retarded the development of the activity and shifted its peak to the 39th day after birth. Conversely, neonatal hyperthyroidism accelerated the entire developmental pattern and shifted the peak to the 16th day after birth. The hydroxylase activity in mouse brain was also increased by thyroid hormone administration from the 13th through the 18th day after birth. Unlike normal mice, the low activity in jimpy mice was not affected by this treatment. It is concluded that thyroid hormones play an important role in the control of brain fatty acid alpha-hydroxylation. The stimulation of alpha-hydroxy fatty acid synthesis in response to hyperthyroidism during the early postnatal period may be one of the major effects of thyroid hormones in accelerating myelination of the central nervous system.", "PMID": 956184}
{"id": "pubmed23n0032_2051", "title": "On the mechanism of regulation of omega oxidation of fatty acids.", "content": "The stimulatory effect of starvation on omega oxidation of stearate by the 20,000 X g supernatant fluid of rat liver homogenates was studied. The effect was obtained after starvation for 24 hours. Starvation for longer times did not further increase omega oxidation. The stimulatory effect of starvation on omega oxidation of stearic acid was accompanied by a reduced incorporation of stearic acid into phosphatidic acid, diglycerides, and triglycerides. Substitution of the 100,000 X g supernatant fluid from liver homogenate of starved rats with 100,000 X g supernatant fluid from liver homogenates of control rats reduced the microsomal omega oxidation of stearic acid with a simultaneous increase in incorporation of stearic acid into the different glycerides. Under the latter conditions almost no free stearic acid could be isolated from the incubation mixture after the incubation. Of three different soluble factors necessary for glyceride formation, ATP appeared to be the most important from a regulatory point of view. Thus the soluble fraction of liver homogenate from a starved rat was shown to contain suboptimal concentrations of ATP. Addition of physiological amounts of ATP to the 20,000 X g supernatant fluid of homogenate of liver of starved rats had the same effect as addition of 100, 000 X g supernatant fluid from liver homogenate of control rats, i.e. decrease in omega oxidation and increase in formation of glycerides. Addition of sn-glycerol 3-phosphate and CoA-SH in amounts optimal for glyceride formation to the 20,000 X g supernatant fluid of liver homogenate of starved rats had only small effects on omega oxidation and glyceride formation. The results are consistent with a competition for free fatty acids between the acyl-CoA synthetases involved in biosynthesis of glycerides and the microsomal hydroxylase(s) involved in omega oxidation of fatty acids. The concentration of ATP in the soluble fraction is of importance in this competition. The possibility is discussed that this competition is of importance also under in vivo conditions and that a decreased rate of esterification in the starved state is responsible for the higher excretion of omega-oxidized fatty acids in urine in the ketotic state.", "contents": "On the mechanism of regulation of omega oxidation of fatty acids. The stimulatory effect of starvation on omega oxidation of stearate by the 20,000 X g supernatant fluid of rat liver homogenates was studied. The effect was obtained after starvation for 24 hours. Starvation for longer times did not further increase omega oxidation. The stimulatory effect of starvation on omega oxidation of stearic acid was accompanied by a reduced incorporation of stearic acid into phosphatidic acid, diglycerides, and triglycerides. Substitution of the 100,000 X g supernatant fluid from liver homogenate of starved rats with 100,000 X g supernatant fluid from liver homogenates of control rats reduced the microsomal omega oxidation of stearic acid with a simultaneous increase in incorporation of stearic acid into the different glycerides. Under the latter conditions almost no free stearic acid could be isolated from the incubation mixture after the incubation. Of three different soluble factors necessary for glyceride formation, ATP appeared to be the most important from a regulatory point of view. Thus the soluble fraction of liver homogenate from a starved rat was shown to contain suboptimal concentrations of ATP. Addition of physiological amounts of ATP to the 20,000 X g supernatant fluid of homogenate of liver of starved rats had the same effect as addition of 100, 000 X g supernatant fluid from liver homogenate of control rats, i.e. decrease in omega oxidation and increase in formation of glycerides. Addition of sn-glycerol 3-phosphate and CoA-SH in amounts optimal for glyceride formation to the 20,000 X g supernatant fluid of liver homogenate of starved rats had only small effects on omega oxidation and glyceride formation. The results are consistent with a competition for free fatty acids between the acyl-CoA synthetases involved in biosynthesis of glycerides and the microsomal hydroxylase(s) involved in omega oxidation of fatty acids. The concentration of ATP in the soluble fraction is of importance in this competition. The possibility is discussed that this competition is of importance also under in vivo conditions and that a decreased rate of esterification in the starved state is responsible for the higher excretion of omega-oxidized fatty acids in urine in the ketotic state.", "PMID": 956185}
{"id": "pubmed23n0032_2052", "title": "Modification of the 5' terminus of mRNA by an RNA (guanine-7-)-methyltransferase from HeLa cells.", "content": "The 5' termini of many viral and cellular mRNAs contain sequences of the type m7G(5\")pppNm. An RNA (guanine-7-)-methyltransferase that specifically methylates the 5'-terminal guanosine residue of RNAs ending in the dinucleoside triphosphate G(5')pppN- has been purified from the cytoplasm of HeLa cells. Approximately two-thirds of the methyltransferase activity detected in an assay employing umnethylated vaccinia virus mRNA as acceptor was located in the cytoplasm when cells were disrupted by Dounce homogenization; 30% of the cytoplasmic activity was associated with ribosomes but was removed by washing with 0.5 M KCl. The enzyme was purified 165-fold from the cytoplasm by removing nucleic acid by phase partition followed by ammonium sulfate precipitation and column chromatography on DEAE-cellulose, denatured DNA-agarose, and CM-Sephadex. The partially purified enzyme preparation methylated heterologous tRNAs as well as vaccinia mRNA, but the tRNA methyltransferases could be separated from the mRNA activity by sucrose gradient sedimentation and gel filtration on Sephadex G-200. The product of the partially purified enzyme using vaccinia mRNA as substrate was exclusively 7-methylguanosine located in the terminal dinucleoside triphosphate. In addition to RNAs and synthetic polyribonucleotides terminating in a dinucleoside triphosphate, free G(5')pppG could be methylated but GTP, GDP, and G(5')pppG could not. The enzyme also methylated the dinucleoside diphosphate G(5')pppG but much less efficiently than G(5')pppG. An S20, W of 3.8, a Stokes radius of 3.6 nm, and a molecular weight of 56,000 were obtained from sucrose gradient sedimentation and Sephadex G-200 column chromatography.", "contents": "Modification of the 5' terminus of mRNA by an RNA (guanine-7-)-methyltransferase from HeLa cells. The 5' termini of many viral and cellular mRNAs contain sequences of the type m7G(5\")pppNm. An RNA (guanine-7-)-methyltransferase that specifically methylates the 5'-terminal guanosine residue of RNAs ending in the dinucleoside triphosphate G(5')pppN- has been purified from the cytoplasm of HeLa cells. Approximately two-thirds of the methyltransferase activity detected in an assay employing umnethylated vaccinia virus mRNA as acceptor was located in the cytoplasm when cells were disrupted by Dounce homogenization; 30% of the cytoplasmic activity was associated with ribosomes but was removed by washing with 0.5 M KCl. The enzyme was purified 165-fold from the cytoplasm by removing nucleic acid by phase partition followed by ammonium sulfate precipitation and column chromatography on DEAE-cellulose, denatured DNA-agarose, and CM-Sephadex. The partially purified enzyme preparation methylated heterologous tRNAs as well as vaccinia mRNA, but the tRNA methyltransferases could be separated from the mRNA activity by sucrose gradient sedimentation and gel filtration on Sephadex G-200. The product of the partially purified enzyme using vaccinia mRNA as substrate was exclusively 7-methylguanosine located in the terminal dinucleoside triphosphate. In addition to RNAs and synthetic polyribonucleotides terminating in a dinucleoside triphosphate, free G(5')pppG could be methylated but GTP, GDP, and G(5')pppG could not. The enzyme also methylated the dinucleoside diphosphate G(5')pppG but much less efficiently than G(5')pppG. An S20, W of 3.8, a Stokes radius of 3.6 nm, and a molecular weight of 56,000 were obtained from sucrose gradient sedimentation and Sephadex G-200 column chromatography.", "PMID": 956186}
{"id": "pubmed23n0032_2053", "title": "Carbohydrate structure of glycopeptides isolated from an hepatic membrane-binding protein specific for asialoglycoproteins.", "content": "Two variant glycopeptide constituents of an hepatic membrane protein responsible for the clearance of circulating asialoglycoproteins have been isolated and characterized. Their relative abundance has been estimated to be 14 and 2 mol, respectively, per mol of intact protein. The larger component, glycopeptide I, is comprised of sialic acid, galactose, mannose, glucosamine, and aspartic acid in a molar ratio of 3:3:2:5:1. The smaller neutral fraction contains only two sugars and the molar relationship of mannose, glucosamine, and aspartic acid is 8:2:1. Based on the results of sequential digestion with specific glycosidases and periodate degradation, a carbohydrate sequence for both glycopeptides is proposed.", "contents": "Carbohydrate structure of glycopeptides isolated from an hepatic membrane-binding protein specific for asialoglycoproteins. Two variant glycopeptide constituents of an hepatic membrane protein responsible for the clearance of circulating asialoglycoproteins have been isolated and characterized. Their relative abundance has been estimated to be 14 and 2 mol, respectively, per mol of intact protein. The larger component, glycopeptide I, is comprised of sialic acid, galactose, mannose, glucosamine, and aspartic acid in a molar ratio of 3:3:2:5:1. The smaller neutral fraction contains only two sugars and the molar relationship of mannose, glucosamine, and aspartic acid is 8:2:1. Based on the results of sequential digestion with specific glycosidases and periodate degradation, a carbohydrate sequence for both glycopeptides is proposed.", "PMID": 956187}
{"id": "pubmed23n0032_2054", "title": "Deacetylation reaction catalyzed by Salmonella phage c341 and its baseplate parts.", "content": "Phage c341 was shown to cleave off 14C-acetyl groups from a 14C-acetylated polysaccharide, prepared by the enzymatic acetylation of alkali-treated Salmonella anatum O-polysaccharide catalyzed by a membrane fraction from S. anatum (O-10 transacetylase). No deacetylation reaction was detected if the substate was prepared from alkali-treated O-polysaccharide of Salmonella newington. The structural difference of these O-polysaccharides is the anomeric configuration of the linkage between the common mannosyl-rhamnosyl-galactose repeating units. A soluble protein obtained from phage c341 lysate, which has previously been identified as the free form of the baseplate parts of this phage, showed the deacetylase activity, a result indicating that the baseplate is responsible for the enzymatic activity of the phage particles. These results suggest that as in the case of other Salmonella phages such as epsilon15, epsilon34, and P22 that contain glycosidases as baseplates, the baseplate deacetylase of c341 plays a role for phage adsorption through the formation of enzyme-substrate type complexes with the receptor O-polysaccharide.", "contents": "Deacetylation reaction catalyzed by Salmonella phage c341 and its baseplate parts. Phage c341 was shown to cleave off 14C-acetyl groups from a 14C-acetylated polysaccharide, prepared by the enzymatic acetylation of alkali-treated Salmonella anatum O-polysaccharide catalyzed by a membrane fraction from S. anatum (O-10 transacetylase). No deacetylation reaction was detected if the substate was prepared from alkali-treated O-polysaccharide of Salmonella newington. The structural difference of these O-polysaccharides is the anomeric configuration of the linkage between the common mannosyl-rhamnosyl-galactose repeating units. A soluble protein obtained from phage c341 lysate, which has previously been identified as the free form of the baseplate parts of this phage, showed the deacetylase activity, a result indicating that the baseplate is responsible for the enzymatic activity of the phage particles. These results suggest that as in the case of other Salmonella phages such as epsilon15, epsilon34, and P22 that contain glycosidases as baseplates, the baseplate deacetylase of c341 plays a role for phage adsorption through the formation of enzyme-substrate type complexes with the receptor O-polysaccharide.", "PMID": 956188}
{"id": "pubmed23n0032_2055", "title": "Physical properties of antitumor glutaminase-asparaginase from Pseudomonas 7A.", "content": "Glutaminase-asparaginase from Pseudomonas 7A appears to have four subunits with a molecular weight of 36,000 +/- 500 by sedimentation equilibrium in 5.9 M guanidine HCl and 34,000 by amino acid analysis. Analytic sedimentation equilibrium of the native enzyme showed a molecular weight of 140,000 +/- 3,300 with no signs of association or dissociation. Moving boundary and zone sedimentation in buffer showed normal behavior with sedimentation coefficients of 7.92 and 7.75 S, respectively. In contrast, the enzyme appeared to polymerize during zone sedimentation when the initial protein concentration was greater than 1 mg/ml and the buffer contained asparagine, glutamine, or 5-diazo-4-oxonorvaline. An extension of our method for active enzyme sedimentation is described which utilizes the changes in absorption during hydrolysis of asparagine by low concentrations of enzyme. Polymerization was not seen under these conditions.", "contents": "Physical properties of antitumor glutaminase-asparaginase from Pseudomonas 7A. Glutaminase-asparaginase from Pseudomonas 7A appears to have four subunits with a molecular weight of 36,000 +/- 500 by sedimentation equilibrium in 5.9 M guanidine HCl and 34,000 by amino acid analysis. Analytic sedimentation equilibrium of the native enzyme showed a molecular weight of 140,000 +/- 3,300 with no signs of association or dissociation. Moving boundary and zone sedimentation in buffer showed normal behavior with sedimentation coefficients of 7.92 and 7.75 S, respectively. In contrast, the enzyme appeared to polymerize during zone sedimentation when the initial protein concentration was greater than 1 mg/ml and the buffer contained asparagine, glutamine, or 5-diazo-4-oxonorvaline. An extension of our method for active enzyme sedimentation is described which utilizes the changes in absorption during hydrolysis of asparagine by low concentrations of enzyme. Polymerization was not seen under these conditions.", "PMID": 956189}
{"id": "pubmed23n0032_2056", "title": "Interaction of human interferons with immobilized hydrophobic amino acids and dipeptides.", "content": "Human fibroblast interferon binds to L-tryptophan, D-tryptophan, L-phenylalanine, and L-tyrosine, all immobilized directly to cyanogen bromide-activated agarose, as well as to L-tryptophan and D-tryptophan methyl ester, both immobilized via molecular arms. The retention of fibroblast interferon is selective and results in a 2300-fold purification. Human leukocyte interferon binds neither to L-tryptophan attached directly to an agarose matrix nor to L-tryptophan immobilized via a molecular arm; it binds, however, to immobilized L-tryptophyl-L-tryptophan and L-tryptophyl-L-tryrosine. When retained, both interferons cannot be displaced unless ethylene glycol is included in the eluant, indicating a hydrophobic interaction. The interaction takes place under physiologic solvent conditions, thus revealing the high intrinsic hydrophobicity of both interferons.", "contents": "Interaction of human interferons with immobilized hydrophobic amino acids and dipeptides. Human fibroblast interferon binds to L-tryptophan, D-tryptophan, L-phenylalanine, and L-tyrosine, all immobilized directly to cyanogen bromide-activated agarose, as well as to L-tryptophan and D-tryptophan methyl ester, both immobilized via molecular arms. The retention of fibroblast interferon is selective and results in a 2300-fold purification. Human leukocyte interferon binds neither to L-tryptophan attached directly to an agarose matrix nor to L-tryptophan immobilized via a molecular arm; it binds, however, to immobilized L-tryptophyl-L-tryptophan and L-tryptophyl-L-tryrosine. When retained, both interferons cannot be displaced unless ethylene glycol is included in the eluant, indicating a hydrophobic interaction. The interaction takes place under physiologic solvent conditions, thus revealing the high intrinsic hydrophobicity of both interferons.", "PMID": 956190}
{"id": "pubmed23n0032_2057", "title": "Chemical properties of two antigens controlled by the major histocompatibility complex of the chicken.", "content": "The chemistry of antigens controlled by the major histocompatibility complex of the chicken has been investigated. Peripheral blood leukocytes of five chicken strains were radioactively labeled by incorporation of tritiated amino acids, enzymatic iodination, or mild periodate oxidation followed by reduction with tritiated sodium borohydride. Membrane-bound antigens were solubilized with Nonidet P-40 and purified by a two-step immunoprecipitation procedure. The resulting immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing or nonreducing conditions. The chicken major histocompatibility antigens (B antigens) are composed of polypeptide chains with molecular weights (in the reduced form) of 40,000 to 43,000 and 11,000 to 12,000, as deduced from their mobilities in internally calibrated gels. The larger chains, when isolated from different chicken strains, can have slightly different mobilities in such gels; thus the mobilities of the large polypeptides from strains WA and WB correspond to molecular weights of 40,000 and 43,000, respectively. Gels run under nonreducing conditions give similar results; in particular no dimers or oligomers of the larger chains are detectable, thus ruling out an immunoglobulin-like structure for the B antigens. The large chains are labeled by the periodate oxidation-reduction procedure, suggesting that they are glycoproteins (probably containing sialic acid), while the small polypeptides cannot be labeled by this method. An additional protein or proteins of apparent molecular weight about 30,000 could be precipitated from 3H-amino-acid-labeled leukocyte lysates by one particular anti-B serum. This protein(s), named B-L, differs from the B major histocompatibility antigens in that it is not associated with the small chain of molecular weight 11,000 to 12,000 and does not occur on erythrocytes. Thus, at least two protein chains are coded for (or have their synthesis controlled by) genes in the chicken major histocompatibility complex.", "contents": "Chemical properties of two antigens controlled by the major histocompatibility complex of the chicken. The chemistry of antigens controlled by the major histocompatibility complex of the chicken has been investigated. Peripheral blood leukocytes of five chicken strains were radioactively labeled by incorporation of tritiated amino acids, enzymatic iodination, or mild periodate oxidation followed by reduction with tritiated sodium borohydride. Membrane-bound antigens were solubilized with Nonidet P-40 and purified by a two-step immunoprecipitation procedure. The resulting immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing or nonreducing conditions. The chicken major histocompatibility antigens (B antigens) are composed of polypeptide chains with molecular weights (in the reduced form) of 40,000 to 43,000 and 11,000 to 12,000, as deduced from their mobilities in internally calibrated gels. The larger chains, when isolated from different chicken strains, can have slightly different mobilities in such gels; thus the mobilities of the large polypeptides from strains WA and WB correspond to molecular weights of 40,000 and 43,000, respectively. Gels run under nonreducing conditions give similar results; in particular no dimers or oligomers of the larger chains are detectable, thus ruling out an immunoglobulin-like structure for the B antigens. The large chains are labeled by the periodate oxidation-reduction procedure, suggesting that they are glycoproteins (probably containing sialic acid), while the small polypeptides cannot be labeled by this method. An additional protein or proteins of apparent molecular weight about 30,000 could be precipitated from 3H-amino-acid-labeled leukocyte lysates by one particular anti-B serum. This protein(s), named B-L, differs from the B major histocompatibility antigens in that it is not associated with the small chain of molecular weight 11,000 to 12,000 and does not occur on erythrocytes. Thus, at least two protein chains are coded for (or have their synthesis controlled by) genes in the chicken major histocompatibility complex.", "PMID": 956191}
{"id": "pubmed23n0032_2058", "title": "Polycentric total knee arthroplasty. A two-year follow-up study.", "content": "Polycentric total knee arthroplasty provided significant relief of pain in 86 per cent of 500 knees. The independence and activity levels of the patients increased dramatically. The frequency of major complications as reflected by reoperation was 10 per cent in this series. There was a 2.8 per cent deep infection rate. One-third of the infected knees were salvaged and two-thirds required arthrodesis. Loosening of a component was noted in 2.4 per cent. After operation the average range of motion was from 6 to 101 degrees of flexion, for a range of 95 degrees; this was a 5-degree increase over average preoperative motion. Ninety-six per cent of the patients expressed satisfaction with the surgical result.", "contents": "Polycentric total knee arthroplasty. A two-year follow-up study. Polycentric total knee arthroplasty provided significant relief of pain in 86 per cent of 500 knees. The independence and activity levels of the patients increased dramatically. The frequency of major complications as reflected by reoperation was 10 per cent in this series. There was a 2.8 per cent deep infection rate. One-third of the infected knees were salvaged and two-thirds required arthrodesis. Loosening of a component was noted in 2.4 per cent. After operation the average range of motion was from 6 to 101 degrees of flexion, for a range of 95 degrees; this was a 5-degree increase over average preoperative motion. Ninety-six per cent of the patients expressed satisfaction with the surgical result.", "PMID": 956217}
{"id": "pubmed23n0032_2059", "title": "Geometric total knee arthroplasty. A two-year follow-up study.", "content": "In a study of our experience with the first 119 geometric arthroplasties of the knee done at the Mayo Clinic, reported after a minimum follow-up period of two years, we noted that relief of pain was the primary goal in 84 per cent of the cases and this was achieved in the majority. The average total motion was relatively unchanged but it became more useful postoperatively.", "contents": "Geometric total knee arthroplasty. A two-year follow-up study. In a study of our experience with the first 119 geometric arthroplasties of the knee done at the Mayo Clinic, reported after a minimum follow-up period of two years, we noted that relief of pain was the primary goal in 84 per cent of the cases and this was achieved in the majority. The average total motion was relatively unchanged but it became more useful postoperatively.", "PMID": 956218}
{"id": "pubmed23n0032_2060", "title": "A comparison of four models of total knee-replacement prostheses.", "content": "Twenty-nine knees with unicondylar, sixty-four with duocondylar, fifty with Guepar, and fifty with geometric prostheses were studied. The follow-up ranged from two to three and one-half years. The unicondylar prosthesis was used in the mildest cases and gave the least complications, but the quality of results was not superior to that achieved with the other prostheses. The duocondylar model was best suited for knees with rheumatoid arthritis and mild deformity. The geometric prosthesis was the best condylar prosthesis for osteoarthritis with moderate to severe deformity, but gave the worst results in knees with rheumatoid arthritis. The Guepar prosthesis was used in the worst knees and gave the best results, but it had the highest infection rate and was the most difficult to salvage. A radiolucency was observed in about 60 per cent of the condylar replacements around the tibial component and in 45 per cent of the Geupar replacements around the femoral component. The significance of this cannot yet be determined but it suggest that the fixation may not be ideal. In all types, residual pain was most frequently attributed to the patellar compartment. Patellectomy was not a solution.", "contents": "A comparison of four models of total knee-replacement prostheses. Twenty-nine knees with unicondylar, sixty-four with duocondylar, fifty with Guepar, and fifty with geometric prostheses were studied. The follow-up ranged from two to three and one-half years. The unicondylar prosthesis was used in the mildest cases and gave the least complications, but the quality of results was not superior to that achieved with the other prostheses. The duocondylar model was best suited for knees with rheumatoid arthritis and mild deformity. The geometric prosthesis was the best condylar prosthesis for osteoarthritis with moderate to severe deformity, but gave the worst results in knees with rheumatoid arthritis. The Guepar prosthesis was used in the worst knees and gave the best results, but it had the highest infection rate and was the most difficult to salvage. A radiolucency was observed in about 60 per cent of the condylar replacements around the tibial component and in 45 per cent of the Geupar replacements around the femoral component. The significance of this cannot yet be determined but it suggest that the fixation may not be ideal. In all types, residual pain was most frequently attributed to the patellar compartment. Patellectomy was not a solution.", "PMID": 956219}
{"id": "pubmed23n0032_2061", "title": "Modular total knee-replacement arthroplasty. A review of eighty-nine patients.", "content": "Fifty-eight osteoarthritic and thirty-one rheumatoid patients underwent modular total knee-replacement arthroplasty. The major indication for the operation was relief of pain. Contraindications to this resurfacing arthroplasty included varus-valgus instability of over 20 degrees, combined varus-valgus instability with flexion contracture of over 40 degrees, marked recurvatum, and predominant patellofemoral symptoms. In 59 per cent of the osteoarthritic and 58 per cent of the rheumatoid patients, complete relief of pain was evident when they were evaluated twenty-four months after surgery, while another 35 per cent of each group had only mild pain related to inclement weather. Their ability to walk long distances without support or limp was increased. Range of motion and ability to climb stairs were not significantly improved.", "contents": "Modular total knee-replacement arthroplasty. A review of eighty-nine patients. Fifty-eight osteoarthritic and thirty-one rheumatoid patients underwent modular total knee-replacement arthroplasty. The major indication for the operation was relief of pain. Contraindications to this resurfacing arthroplasty included varus-valgus instability of over 20 degrees, combined varus-valgus instability with flexion contracture of over 40 degrees, marked recurvatum, and predominant patellofemoral symptoms. In 59 per cent of the osteoarthritic and 58 per cent of the rheumatoid patients, complete relief of pain was evident when they were evaluated twenty-four months after surgery, while another 35 per cent of each group had only mild pain related to inclement weather. Their ability to walk long distances without support or limp was increased. Range of motion and ability to climb stairs were not significantly improved.", "PMID": 956220}
{"id": "pubmed23n0032_2062", "title": "Failed polycentric total knee prostheses.", "content": "Four failed polycentric total knee prostheses were evaluated by clinical examination of the patients and surface analysis of the removed prosthetic components. The failures were secondary to a poor relationship of the prosthetic components in three cases and to abutment of the tibial spine against the femoral condyle in the fourth. A poor relationship of the prosthetic components was not necessarily secondary to malpositioning, because the positioning may have been accurate but the relationships became abnormal when the knee was unstable. Scanning electron-microscope examination revealed various wear patterns but no significant correlation was noted between the morphological type and the degree of wear. Accurate placement of the components and restoration of knee stability were critical requirements for the polycentric total knee prosthesis to be successful.", "contents": "Failed polycentric total knee prostheses. Four failed polycentric total knee prostheses were evaluated by clinical examination of the patients and surface analysis of the removed prosthetic components. The failures were secondary to a poor relationship of the prosthetic components in three cases and to abutment of the tibial spine against the femoral condyle in the fourth. A poor relationship of the prosthetic components was not necessarily secondary to malpositioning, because the positioning may have been accurate but the relationships became abnormal when the knee was unstable. Scanning electron-microscope examination revealed various wear patterns but no significant correlation was noted between the morphological type and the degree of wear. Accurate placement of the components and restoration of knee stability were critical requirements for the polycentric total knee prosthesis to be successful.", "PMID": 956221}
{"id": "pubmed23n0032_2063", "title": "Patterns of movement of totally intrinsic-minus fingers based on a study of one hundred and forty-one fingers.", "content": "A detailed study of metacarpophalangeal flexion and interphalangeal extension movements of 141 fingers with complete intrinsic-muscle paralysis due to leprosy showed that long flexors and long extensors produce movement at the metacarpophalangeal and proximal interphalangeal joints simultaneously, and not successively as is generally believed. The amounts of flexion resulting from long flexor activity are almost equal at the two joints and metacarpophalangeal flexion is achieved without excessive flexion of the proximal interphalangeal joint, but this is masked by the claw-finger deformity. The movement resulting from activity of the long extensor is complex and there are three or more qualitatively different patterns of extension. Although the long extensor produces simultaneous extension at the metacarpophalangeal and proximal interphalangeal joints, the latter consistently lags behind the former so that full extension is not achieved at the proximal interphalangeal joint even when the metacarpophalangeal joint is maximally extended. The diverse patterns of extension are not related to duration of degree of clawing or to any particular finger.", "contents": "Patterns of movement of totally intrinsic-minus fingers based on a study of one hundred and forty-one fingers. A detailed study of metacarpophalangeal flexion and interphalangeal extension movements of 141 fingers with complete intrinsic-muscle paralysis due to leprosy showed that long flexors and long extensors produce movement at the metacarpophalangeal and proximal interphalangeal joints simultaneously, and not successively as is generally believed. The amounts of flexion resulting from long flexor activity are almost equal at the two joints and metacarpophalangeal flexion is achieved without excessive flexion of the proximal interphalangeal joint, but this is masked by the claw-finger deformity. The movement resulting from activity of the long extensor is complex and there are three or more qualitatively different patterns of extension. Although the long extensor produces simultaneous extension at the metacarpophalangeal and proximal interphalangeal joints, the latter consistently lags behind the former so that full extension is not achieved at the proximal interphalangeal joint even when the metacarpophalangeal joint is maximally extended. The diverse patterns of extension are not related to duration of degree of clawing or to any particular finger.", "PMID": 956222}
{"id": "pubmed23n0032_2064", "title": "Evaluation of the two-stage flexor-tendon reconstruction in severely damaged digits.", "content": "From July 1971 to July 1974, the two-stage tendon-grafting procedure of Hunter and Salisbury was performed in thirty-two severely damaged digits in twenty-five patients. Of these digits, twenty-three fingers and five thumbs could be evaluated for gain in total active flexion (expressed as per cent of preoperative passive flexion) and for gain in total active motion (expressed as per cent of total preoperative passive motion) after follow-ups ranging from six to fifty months. The results for total active flexion were 60.9 per cent good, 21.7 per cent fair, and 17.4 per cent poor, and for total active motion 21.7 per cent good, 56.5 per cent fair, and 21.7 per cent poor. Complications were frequent after both stages and included infection, migration of the rod, and adhesions within the proximal end of the newly formed sheath. Flexion contractures were a significant problem.", "contents": "Evaluation of the two-stage flexor-tendon reconstruction in severely damaged digits. From July 1971 to July 1974, the two-stage tendon-grafting procedure of Hunter and Salisbury was performed in thirty-two severely damaged digits in twenty-five patients. Of these digits, twenty-three fingers and five thumbs could be evaluated for gain in total active flexion (expressed as per cent of preoperative passive flexion) and for gain in total active motion (expressed as per cent of total preoperative passive motion) after follow-ups ranging from six to fifty months. The results for total active flexion were 60.9 per cent good, 21.7 per cent fair, and 17.4 per cent poor, and for total active motion 21.7 per cent good, 56.5 per cent fair, and 21.7 per cent poor. Complications were frequent after both stages and included infection, migration of the rod, and adhesions within the proximal end of the newly formed sheath. Flexion contractures were a significant problem.", "PMID": 956223}
{"id": "pubmed23n0032_2065", "title": "Late reconstruction of the ligaments following acromioclavicular separation.", "content": "Complete separation of the acromion from the clavicle in nine young patients was repaired by a new technique with use of a tendon graft. The operation was performed, on average, twenty-nine weeks after the injury. After surgery seven patients were enabled to engage in strenuous sports or construction work and the other two to return to daily activities with occasional heavy lifting.", "contents": "Late reconstruction of the ligaments following acromioclavicular separation. Complete separation of the acromion from the clavicle in nine young patients was repaired by a new technique with use of a tendon graft. The operation was performed, on average, twenty-nine weeks after the injury. After surgery seven patients were enabled to engage in strenuous sports or construction work and the other two to return to daily activities with occasional heavy lifting.", "PMID": 956224}
{"id": "pubmed23n0032_2066", "title": "Compensating osteotomy at the base of the femoral neck for slipped capital femoral epiphysis.", "content": "A compensating osteotomy for correction of the varus and retroversion components of severe slipped capital femoral epiphysis is presented. It is safer than a cuneiform osteotomy done through the callus of the slipped epiphysis because the line of osteotomy is distal to the major blood supply of the posterior retinaculum. The threaded Steinmann pins used for fixation sross both the osteotomy site and the epiphyseal plate, and therefore not only are the mechanical relationships restored, but further slipping is prevented also.", "contents": "Compensating osteotomy at the base of the femoral neck for slipped capital femoral epiphysis. A compensating osteotomy for correction of the varus and retroversion components of severe slipped capital femoral epiphysis is presented. It is safer than a cuneiform osteotomy done through the callus of the slipped epiphysis because the line of osteotomy is distal to the major blood supply of the posterior retinaculum. The threaded Steinmann pins used for fixation sross both the osteotomy site and the epiphyseal plate, and therefore not only are the mechanical relationships restored, but further slipping is prevented also.", "PMID": 956225}
{"id": "pubmed23n0032_2067", "title": "Stiffness of the proximal interphalangeal joints in rheumatoid arthritis. The role of flexor tenosynovitis.", "content": "In nineteen hands (seventy-four fingers) of eleven women and one man with rheumatoid arthritis there was restriction of active and passive motion of the proximal interphalangeal joints, with signs of flexor tenosynovitis but no clinical or roentgenographic evidence of involvement of the joint. The nineteen hands were treated by flexor tenosynovectomy (palm only in nine, palm and carpal tunnel in five, both palm and digits in four, and digit, palm, and wrist in one) combined with manipulation of the joint under regional anesthesia. After an average follow-up of twenty-one months (range, six to thirty-six months), the average range of active motion had increased from 40 to 84 degrees and the average range of passive motions, from 57 to 87 degrees. Only three patients had unsatisfactory results, one because of persistent unexplained swelling and two because of recurrence of the tenosynovitis.", "contents": "Stiffness of the proximal interphalangeal joints in rheumatoid arthritis. The role of flexor tenosynovitis. In nineteen hands (seventy-four fingers) of eleven women and one man with rheumatoid arthritis there was restriction of active and passive motion of the proximal interphalangeal joints, with signs of flexor tenosynovitis but no clinical or roentgenographic evidence of involvement of the joint. The nineteen hands were treated by flexor tenosynovectomy (palm only in nine, palm and carpal tunnel in five, both palm and digits in four, and digit, palm, and wrist in one) combined with manipulation of the joint under regional anesthesia. After an average follow-up of twenty-one months (range, six to thirty-six months), the average range of active motion had increased from 40 to 84 degrees and the average range of passive motions, from 57 to 87 degrees. Only three patients had unsatisfactory results, one because of persistent unexplained swelling and two because of recurrence of the tenosynovitis.", "PMID": 956226}
{"id": "pubmed23n0032_2068", "title": "Milwaukee brace correction of idiopathic scoliosis. A biomechanical analysis and a restrospective study.", "content": "We analyzed the biomechanics of Milwaukee brace treatment of idiopathic scoliotic patients through simulation in five computer-constructed model spines. The contributions to correction of each component of the brace were examined in these model spines, and some of the mechanical principles that determine the outcome of brace treatment were studied. The validity of the stimulation findings was then tested by a retrospective analysis. Simulation was used to predict the outcome of milwaukee brace treatment in sixty-eight patients. In 81 per cent of these patients, the actual outcome agreed with the prediction. The study suggests that careful adherence to mechanical principles in the use of a Milwaukee brace will result in successful treatment of more patients.", "contents": "Milwaukee brace correction of idiopathic scoliosis. A biomechanical analysis and a restrospective study. We analyzed the biomechanics of Milwaukee brace treatment of idiopathic scoliotic patients through simulation in five computer-constructed model spines. The contributions to correction of each component of the brace were examined in these model spines, and some of the mechanical principles that determine the outcome of brace treatment were studied. The validity of the stimulation findings was then tested by a retrospective analysis. Simulation was used to predict the outcome of milwaukee brace treatment in sixty-eight patients. In 81 per cent of these patients, the actual outcome agreed with the prediction. The study suggests that careful adherence to mechanical principles in the use of a Milwaukee brace will result in successful treatment of more patients.", "PMID": 956227}
{"id": "pubmed23n0032_2069", "title": "Tenderness at motor points. A diagnostic and prognostic aid for low-back injury.", "content": "In patients with low-back injury the motor points of some muscles may be tender. Of fifty patients with low-back \"strain\", twenty-six had tender motor points and twenty-four did not, while forty-nine of fifty patients with radicular signs and symptoms suggesting disc involvement had tender motor points, and the one without such tender points had a hamstring contusion which limited straight leg raising. Of fifty controls with no back disability, only seven had mild tender points after strenuous activity, while forty-six of another fifty controls with occasional back discomfort had mild motor-point tenderness. In all instances the tender motor points were located in the myotomes corresponding to the probable segmental levels of spinal injury and of root involvement, when present. Patients with low-back strain and no tender motor points were disabled for an average of 6.9 weeks, while those with the same diagnosis but tender motor points were disabled for an average of 19.7 weeks, or almost as long as the patients with signs of radicular involvement, who were disabled for an average of 25.7 weeks. Tender motor points may therefore be of diagnostic and prognostic value, serving as sensitive localizers of radicular involvement and differentiating a simple mechanical low-back strain from one with neural involvement.", "contents": "Tenderness at motor points. A diagnostic and prognostic aid for low-back injury. In patients with low-back injury the motor points of some muscles may be tender. Of fifty patients with low-back \"strain\", twenty-six had tender motor points and twenty-four did not, while forty-nine of fifty patients with radicular signs and symptoms suggesting disc involvement had tender motor points, and the one without such tender points had a hamstring contusion which limited straight leg raising. Of fifty controls with no back disability, only seven had mild tender points after strenuous activity, while forty-six of another fifty controls with occasional back discomfort had mild motor-point tenderness. In all instances the tender motor points were located in the myotomes corresponding to the probable segmental levels of spinal injury and of root involvement, when present. Patients with low-back strain and no tender motor points were disabled for an average of 6.9 weeks, while those with the same diagnosis but tender motor points were disabled for an average of 19.7 weeks, or almost as long as the patients with signs of radicular involvement, who were disabled for an average of 25.7 weeks. Tender motor points may therefore be of diagnostic and prognostic value, serving as sensitive localizers of radicular involvement and differentiating a simple mechanical low-back strain from one with neural involvement.", "PMID": 956228}
{"id": "pubmed23n0032_2070", "title": "Total hip reconstruction in chronically dislocated hips.", "content": "The pathological anatomy of chronically dislocated hips makes reconstruction for more difficult in them than in most cases. The acetabular component must be seated at the site of the original triradiate cartilage and the femur must be shortened four or more centimeters to prevent excessive limb lengthening. This means that the femoral component must be seated in the smallest, strightest portion of the intramedullary canal. A specially designed prosthesis is often needed. Twenty-two hips were operated on in this series, and all patients had relief of pain and improvement of gait. One major complication occured: a sciatic-nerve palsy due to overlengthening of the femur and improper postoperative positioning.", "contents": "Total hip reconstruction in chronically dislocated hips. The pathological anatomy of chronically dislocated hips makes reconstruction for more difficult in them than in most cases. The acetabular component must be seated at the site of the original triradiate cartilage and the femur must be shortened four or more centimeters to prevent excessive limb lengthening. This means that the femoral component must be seated in the smallest, strightest portion of the intramedullary canal. A specially designed prosthesis is often needed. Twenty-two hips were operated on in this series, and all patients had relief of pain and improvement of gait. One major complication occured: a sciatic-nerve palsy due to overlengthening of the femur and improper postoperative positioning.", "PMID": 956229}
{"id": "pubmed23n0032_2071", "title": "Pyogenic arthritis of the sacro-iliac joint. Long-term follow-up.", "content": "Nine cases of sacro-iliac pyarthrosis are presented. The difficulty in localizing the infection is attributable mostly to failure to appreciate the posteriorly situated physical findings. This, and the difficulty with early roentgenographic demonstration of the lesion, may lead to unnecessary abdominal exploration (as in two of our patients) or to prolonged delay in diagnosis and hence spread of the infection. Awareness of the usual physical findings and prompt use of radioisotope scanning to localize the infection led to earlier diagnosis and avoidance of surgery in three patients seen recently. Antibiotic therapy, with or without surgery, led to cure in all patients, with minimum sequelae.", "contents": "Pyogenic arthritis of the sacro-iliac joint. Long-term follow-up. Nine cases of sacro-iliac pyarthrosis are presented. The difficulty in localizing the infection is attributable mostly to failure to appreciate the posteriorly situated physical findings. This, and the difficulty with early roentgenographic demonstration of the lesion, may lead to unnecessary abdominal exploration (as in two of our patients) or to prolonged delay in diagnosis and hence spread of the infection. Awareness of the usual physical findings and prompt use of radioisotope scanning to localize the infection led to earlier diagnosis and avoidance of surgery in three patients seen recently. Antibiotic therapy, with or without surgery, led to cure in all patients, with minimum sequelae.", "PMID": 956230}
{"id": "pubmed23n0032_2072", "title": "The use of the Zuelzer hook plate in fixation of olecranon fractures.", "content": "Twenty-five cases of fracture of the olecranon process were treated using a modification of the Zuelzer hook plate, and uniformly excellent or good results were obtained in all cases in which there were no other associated traumatic lesions about the elbow joint. Regardless of the degree of comminution of the fracture, obliquity of the fracture line, or age of the patient, almost a full range of motion was obtained in every case. No failure of the appliance or loss of position occurred in any case despite the fact that active motion was usually instituted in one to two weeks.", "contents": "The use of the Zuelzer hook plate in fixation of olecranon fractures. Twenty-five cases of fracture of the olecranon process were treated using a modification of the Zuelzer hook plate, and uniformly excellent or good results were obtained in all cases in which there were no other associated traumatic lesions about the elbow joint. Regardless of the degree of comminution of the fracture, obliquity of the fracture line, or age of the patient, almost a full range of motion was obtained in every case. No failure of the appliance or loss of position occurred in any case despite the fact that active motion was usually instituted in one to two weeks.", "PMID": 956231}
{"id": "pubmed23n0032_2073", "title": "An intramedullary fixation device for the proximal part of the femur. Nine years' experience.", "content": "Nine years' experience with a combined intramedullary rod and triflanged nail in eighty-four non-pathological fractures in the subtrochanteric region of the femur is reported and a system of classifying these fractures based on morphology is offered. The appliance was successful in permitting early mobilization of patients and afforded a high rate of union of the fractures. The problems of varus displacement of the distal fragment and protrusion of the device into the joint were not encountered, and there was only one mechanical failure.", "contents": "An intramedullary fixation device for the proximal part of the femur. Nine years' experience. Nine years' experience with a combined intramedullary rod and triflanged nail in eighty-four non-pathological fractures in the subtrochanteric region of the femur is reported and a system of classifying these fractures based on morphology is offered. The appliance was successful in permitting early mobilization of patients and afforded a high rate of union of the fractures. The problems of varus displacement of the distal fragment and protrusion of the device into the joint were not encountered, and there was only one mechanical failure.", "PMID": 956232}
{"id": "pubmed23n0032_2074", "title": "Primary synovial chondromatosis of the ankle. A case report.", "content": "A case of primary diffuse synovial chondromatosis of the ankle with long-term follow-up is reported. Trauma is implicated as a precipitating factor. This unusual, distinctive synovial neoplasm presents readily recognizable pathological features (Fig. 2). Specific diagnostic criteria are presented. The differential diagnosis of primary synovial chondromatosis includes secondary reactive synovial chondromatosis and synovial chondrosarcoma. The usual sources of osteochondritic loose bodies, on the other hand, are traumatic joint disruption, osteoarthritis, and infectious disorders. Acute primary synovial chondromatosis is adequately treated by removal of the loose bodies and synovectomy. Postoperative prognosis is dependent on the stage of the disease at the time of surgery. In this acute case, the patient had minimum impairment, and ankle roentgenograms twenty years after the arthrotomy were normal (Fig. 3).", "contents": "Primary synovial chondromatosis of the ankle. A case report. A case of primary diffuse synovial chondromatosis of the ankle with long-term follow-up is reported. Trauma is implicated as a precipitating factor. This unusual, distinctive synovial neoplasm presents readily recognizable pathological features (Fig. 2). Specific diagnostic criteria are presented. The differential diagnosis of primary synovial chondromatosis includes secondary reactive synovial chondromatosis and synovial chondrosarcoma. The usual sources of osteochondritic loose bodies, on the other hand, are traumatic joint disruption, osteoarthritis, and infectious disorders. Acute primary synovial chondromatosis is adequately treated by removal of the loose bodies and synovectomy. Postoperative prognosis is dependent on the stage of the disease at the time of surgery. In this acute case, the patient had minimum impairment, and ankle roentgenograms twenty years after the arthrotomy were normal (Fig. 3).", "PMID": 956234}
{"id": "pubmed23n0032_2075", "title": "The results of early treatment of typical congenital dislocation of the hip in Malm\u00f6.", "content": "The late results of early treatment of congenital dislocation of the hip in the von Rosen splint have been recorded in a follow-up study of 111 children with an average age of ten years (range eight to sixteen). The clinical diagnosis had been made within two days of birth and in most cases confirmed by radiological examination. Splintage was in all cases begun within the first few days and maintained for an average period of ten weeks. Two cases of failure of the initial treatment were encountered but had good final results. The follow-up study included a radiological examination in which were recorded the general configuration of the joint, the CE-angle described by Wiberg, the presence or absence of an os acetabuli and the roundness of the femoral head. The findings were compared with those in a control group of 222 normal children. All the treated hips developed normally except for a single case of minor dysplasia and a single case of slight enlargement of the femoral head and neck following appearances suggestive of avascular necrosis at the age of two years.", "contents": "The results of early treatment of typical congenital dislocation of the hip in Malm\u00f6. The late results of early treatment of congenital dislocation of the hip in the von Rosen splint have been recorded in a follow-up study of 111 children with an average age of ten years (range eight to sixteen). The clinical diagnosis had been made within two days of birth and in most cases confirmed by radiological examination. Splintage was in all cases begun within the first few days and maintained for an average period of ten weeks. Two cases of failure of the initial treatment were encountered but had good final results. The follow-up study included a radiological examination in which were recorded the general configuration of the joint, the CE-angle described by Wiberg, the presence or absence of an os acetabuli and the roundness of the femoral head. The findings were compared with those in a control group of 222 normal children. All the treated hips developed normally except for a single case of minor dysplasia and a single case of slight enlargement of the femoral head and neck following appearances suggestive of avascular necrosis at the age of two years.", "PMID": 956242}
{"id": "pubmed23n0032_2076", "title": "Patello-femoral joint mechanics and pathology. 1. Functional anatomy of the patello-femoral joint.", "content": "Cadaver knee joints were mounted so that life-like forces of weight-bearing were simulated. The patello-femoral contact areas were defined under load throughout the range of movement by the dye method. During movement from extension to 90 degrees of flexion a band of contact sweeps across the patella from inferior to superior pole, but the odd facet makes no contact. At about 135 degrees of flexion separate medial and lateral contact areas form, the medial one limited to the odd facet. From extension to 90 degrees of flexion the patella holds the quadriceps tendon away from the femur, but in further degrees of flexion an extensive \"tendo-femoral\" contact area forms. Between 90 degrees and 135 degrees of flexion the patella rotates and the ridge between the medial and odd facets engages the femoral condyle. The odd facet is shown to be a habitual non-contact area and the ridge to be subject to high load, observations which correlate with cartilage lesions described in Part 2 of the paper.", "contents": "Patello-femoral joint mechanics and pathology. 1. Functional anatomy of the patello-femoral joint. Cadaver knee joints were mounted so that life-like forces of weight-bearing were simulated. The patello-femoral contact areas were defined under load throughout the range of movement by the dye method. During movement from extension to 90 degrees of flexion a band of contact sweeps across the patella from inferior to superior pole, but the odd facet makes no contact. At about 135 degrees of flexion separate medial and lateral contact areas form, the medial one limited to the odd facet. From extension to 90 degrees of flexion the patella holds the quadriceps tendon away from the femur, but in further degrees of flexion an extensive \"tendo-femoral\" contact area forms. Between 90 degrees and 135 degrees of flexion the patella rotates and the ridge between the medial and odd facets engages the femoral condyle. The odd facet is shown to be a habitual non-contact area and the ridge to be subject to high load, observations which correlate with cartilage lesions described in Part 2 of the paper.", "PMID": 956243}
{"id": "pubmed23n0032_2077", "title": "Patello-femoral joint mechanics and pathology. 2. Chondromalacia patellae.", "content": "Two distinct lesions affect the articular cartilage of the patella. Surface degeneration occurs particularly on the odd facet; it is age dependent, often present in youth and it becomes more frequent with increasing age. It probably does not occasion patello-femoral pain in youth, but may predispose to degenerative arthritis in that joint in later years and is regarded as a consequence of habitual disuse. The term \"basal degeneration\" is used to describe a lesion in which there is a fasciculation of collagen in the middle and deep zones of cartilage without, at first, affecting the surface. It was found astride the ridge separating the medial from the odd facet in twenty-three adolescents who had complained of prolonged patello-femoral pain. They were treated by excision of the disc of affected cartilage, with relief of pain in most cases. The pathogenesis of basal degeneration is related to the functional anatomy of the patella.", "contents": "Patello-femoral joint mechanics and pathology. 2. Chondromalacia patellae. Two distinct lesions affect the articular cartilage of the patella. Surface degeneration occurs particularly on the odd facet; it is age dependent, often present in youth and it becomes more frequent with increasing age. It probably does not occasion patello-femoral pain in youth, but may predispose to degenerative arthritis in that joint in later years and is regarded as a consequence of habitual disuse. The term \"basal degeneration\" is used to describe a lesion in which there is a fasciculation of collagen in the middle and deep zones of cartilage without, at first, affecting the surface. It was found astride the ridge separating the medial from the odd facet in twenty-three adolescents who had complained of prolonged patello-femoral pain. They were treated by excision of the disc of affected cartilage, with relief of pain in most cases. The pathogenesis of basal degeneration is related to the functional anatomy of the patella.", "PMID": 956244}
{"id": "pubmed23n0032_2078", "title": "The Shiers arthroplasty of the knee.", "content": "Thirty-one patients with rheumatoid arthritis were reviewed after Shiers arthroplasty of the knee joint for which the main indication was intractable pain. Seven patients had the operation done to both knees. This review was done to assess the long-term results two to seven years later. All patients were clinically and radiologically assessed, and our results showed that pain and instability recurred within eighteen months. Thus it is likely that a prosthesis which allows rotation, and in which the bearing surfaces are metal on plastic, will replace the Shiers prosthesis.", "contents": "The Shiers arthroplasty of the knee. Thirty-one patients with rheumatoid arthritis were reviewed after Shiers arthroplasty of the knee joint for which the main indication was intractable pain. Seven patients had the operation done to both knees. This review was done to assess the long-term results two to seven years later. All patients were clinically and radiologically assessed, and our results showed that pain and instability recurred within eighteen months. Thus it is likely that a prosthesis which allows rotation, and in which the bearing surfaces are metal on plastic, will replace the Shiers prosthesis.", "PMID": 956245}
{"id": "pubmed23n0032_2079", "title": "Pseudoarthrosis after spinal fusion for scoliosis.", "content": "A series of 246 patients with scoliosis and attempted fusion had exploration performed six months later in order to detect and treat any pseudarthrosis at an early stage and so prevent subsequent loss of correction. Bilateral or unilateral pseudarthroses occurred in 25 per cent and were of three types--definite, hairline and doubtful. Single unilateral pseudarthroses accounted for 6 per cent and were of little if any clinical significance. The hairline pseudarthroses could not be seen radiologically and were easily missed at exploration. In general the pseudarthroses were least common in the more rigid parts of the spine and in curves which by nature of their aetiology or long duration had become most rigid. Neither the initial severity of the curve nor the degree of correction obtained before the initial attempted fusion had any apparent effect on the incidence. Follow-up for an average of four years has shown that a pseudarthrosis is of little significance with regard to the ultimate result provided it is recognised early and repaired.", "contents": "Pseudoarthrosis after spinal fusion for scoliosis. A series of 246 patients with scoliosis and attempted fusion had exploration performed six months later in order to detect and treat any pseudarthrosis at an early stage and so prevent subsequent loss of correction. Bilateral or unilateral pseudarthroses occurred in 25 per cent and were of three types--definite, hairline and doubtful. Single unilateral pseudarthroses accounted for 6 per cent and were of little if any clinical significance. The hairline pseudarthroses could not be seen radiologically and were easily missed at exploration. In general the pseudarthroses were least common in the more rigid parts of the spine and in curves which by nature of their aetiology or long duration had become most rigid. Neither the initial severity of the curve nor the degree of correction obtained before the initial attempted fusion had any apparent effect on the incidence. Follow-up for an average of four years has shown that a pseudarthrosis is of little significance with regard to the ultimate result provided it is recognised early and repaired.", "PMID": 956246}
{"id": "pubmed23n0032_2080", "title": "Steroid-induced avascular necrosis of the head of the humerus. Natural history and management.", "content": "Ninety-five patients with steroid-induced avascular necrosis of bone have been personally treated by the author. Of these, eighteen had a lesion of the head of the humerus, on one or both sides. The conditions for which the steroids were given included post-transplantation, lupus erythematosus, glomerulonephritis and asthma. The characteristic lesion began as a subchondral osteolytic area which frequently progressed to collapse. The articular cartilage divided from the subchondral bone, either becoming detached as a free cap or at a later stage reattaching. In some cases the lesion was minimal and the symptoms were slight. Conservative treatment has consisted of pendulum exercises and avoidance of abduction, particularly against resistance. In fourteen patients this led to satisfactory function with only intermittent symptoms. Four patients required replacement of five humeral heads with Neer's prostheses. After one to seven years the results of all five were classified as excellent in terms of absence of symptoms and a free range of movement.", "contents": "Steroid-induced avascular necrosis of the head of the humerus. Natural history and management. Ninety-five patients with steroid-induced avascular necrosis of bone have been personally treated by the author. Of these, eighteen had a lesion of the head of the humerus, on one or both sides. The conditions for which the steroids were given included post-transplantation, lupus erythematosus, glomerulonephritis and asthma. The characteristic lesion began as a subchondral osteolytic area which frequently progressed to collapse. The articular cartilage divided from the subchondral bone, either becoming detached as a free cap or at a later stage reattaching. In some cases the lesion was minimal and the symptoms were slight. Conservative treatment has consisted of pendulum exercises and avoidance of abduction, particularly against resistance. In fourteen patients this led to satisfactory function with only intermittent symptoms. Four patients required replacement of five humeral heads with Neer's prostheses. After one to seven years the results of all five were classified as excellent in terms of absence of symptoms and a free range of movement.", "PMID": 956247}
{"id": "pubmed23n0032_2081", "title": "Anterior cervical subluxation.", "content": "Anterior subluxation of the cervical spine from flexion-rotational violence is often overlooked. Either radiography is omitted or the slight displacement visible on the films is not recognised. A method for recognising these injuries is suggested. Five cases are described showing that in these subluxations the soft-tissue damage is severe, that late increasing displacement can occur, with varying neurological sequelae, and that instability can persist indefinitely. Four of the patients required stabilisation by operation, in contrast with complete dislocations in which 80 to 90 per cent fuse spontaneously.", "contents": "Anterior cervical subluxation. Anterior subluxation of the cervical spine from flexion-rotational violence is often overlooked. Either radiography is omitted or the slight displacement visible on the films is not recognised. A method for recognising these injuries is suggested. Five cases are described showing that in these subluxations the soft-tissue damage is severe, that late increasing displacement can occur, with varying neurological sequelae, and that instability can persist indefinitely. Four of the patients required stabilisation by operation, in contrast with complete dislocations in which 80 to 90 per cent fuse spontaneously.", "PMID": 956248}
{"id": "pubmed23n0032_2082", "title": "Hidden flexion injury of the cervical spine.", "content": "This paper describes seven patients who developed late vertebral deformity after flexion injuries of the cervical spine. In four the clinical and radiological features were subtle and because the patients walked into an emergency department the severity of the injury was not initially appreciated. Certain specific clinical and radiological features of flexion injury are described and emphasis is placed on the importance of correct management. A radiological tetrad is described which should alert the surgeon to the possibility of damage to the posterior interspinous complex of the cervical spine and so lead to further radiological investigations. Despite the frequency of flexion injuries the alarming complications described in this paper are rare.", "contents": "Hidden flexion injury of the cervical spine. This paper describes seven patients who developed late vertebral deformity after flexion injuries of the cervical spine. In four the clinical and radiological features were subtle and because the patients walked into an emergency department the severity of the injury was not initially appreciated. Certain specific clinical and radiological features of flexion injury are described and emphasis is placed on the importance of correct management. A radiological tetrad is described which should alert the surgeon to the possibility of damage to the posterior interspinous complex of the cervical spine and so lead to further radiological investigations. Despite the frequency of flexion injuries the alarming complications described in this paper are rare.", "PMID": 956249}
{"id": "pubmed23n0032_2083", "title": "Seat belt fractures of the cervical spine.", "content": "Three unusual injuries of the cervical spine in wearers of safety belts are reported. In each case, the presence of diagonal bruising and abrasions in the line of the sash strap indicated its involvement in the mechanism of the injury.", "contents": "Seat belt fractures of the cervical spine. Three unusual injuries of the cervical spine in wearers of safety belts are reported. In each case, the presence of diagonal bruising and abrasions in the line of the sash strap indicated its involvement in the mechanism of the injury.", "PMID": 956250}
{"id": "pubmed23n0032_2084", "title": "Legg-Calv\u00e9-Perthes' disease. Morphological studies in two cases examined at necropsy.", "content": "Specimens of femoral heads were studied at necropsy in two cases of Legg-Calve-Perthes' disease. One was that of a boy aged four years ten months who died from appendicitis; the other was from a boy aged six years who died from a malignant glioma. Both had been treated for one and a half years for Legg-Calve-Perthes' disease which was in a stage of repair at the time of death. The diseased femoral heads were moderately flattened but the surface cartilage was intact. Epiphysial bone and bone marrow were partly replaced by cartilage, fibrous tissue and granulation tissue, and new bone was being formed. Inflammatory reaction was inconspicuous. Enchondral bone formation was only slightly decreased, and the structure of the growth plate was undisturbed. There was no sign of systemic bone disease. In the first case the changes indicated that more than one episode of ischaemia had occurred, and an occlusion--probably from an old thrombus--was demonstrated in the posterior inferior retinacular artery of the femoral head. The last episode of ischaemia, furthermore, had caused infarction of part of the metaphysial bone. In both cases, the central area of the metaphysial bone of the affected femur contained fat, but there were few haemopoietic cells and it therefore looked pale. The findings are discussed in relation to previous work on the pathology in Legg-Calve-Perthes' disease, recent information on the vascularisation of the femoral head in children, and experimental and comparative animal studies.", "contents": "Legg-Calv\u00e9-Perthes' disease. Morphological studies in two cases examined at necropsy. Specimens of femoral heads were studied at necropsy in two cases of Legg-Calve-Perthes' disease. One was that of a boy aged four years ten months who died from appendicitis; the other was from a boy aged six years who died from a malignant glioma. Both had been treated for one and a half years for Legg-Calve-Perthes' disease which was in a stage of repair at the time of death. The diseased femoral heads were moderately flattened but the surface cartilage was intact. Epiphysial bone and bone marrow were partly replaced by cartilage, fibrous tissue and granulation tissue, and new bone was being formed. Inflammatory reaction was inconspicuous. Enchondral bone formation was only slightly decreased, and the structure of the growth plate was undisturbed. There was no sign of systemic bone disease. In the first case the changes indicated that more than one episode of ischaemia had occurred, and an occlusion--probably from an old thrombus--was demonstrated in the posterior inferior retinacular artery of the femoral head. The last episode of ischaemia, furthermore, had caused infarction of part of the metaphysial bone. In both cases, the central area of the metaphysial bone of the affected femur contained fat, but there were few haemopoietic cells and it therefore looked pale. The findings are discussed in relation to previous work on the pathology in Legg-Calve-Perthes' disease, recent information on the vascularisation of the femoral head in children, and experimental and comparative animal studies.", "PMID": 956251}
{"id": "pubmed23n0032_2085", "title": "Overgrowth of the femur after fracture of the shaft in childhood.", "content": "Twenty-six children conservatively treated for fracture of the femoral shaft have been reviewed with regard to differences in limb length seven to ten years after the injury. In nine patients the clinical measurements were checked against radiographic measurements of both femora and tibiae. The following conclusions were reached. Nearly two-thirds of the patients had overgrowth of the femur of 10 millimetres or more. Shortening of 15 to 20 millimetres at the fracture site was well compensated for by accelerated growth. Growth acceleration seemed to take place during the healing period and the difference at the end of healing was permanent. Overgrowth was promoted by comminuted and long oblique fractures and by overriding of the fracture ends, but was not influenced by the age at fracture, the duration of treatment or the level of fracture of the shaft. Growth of the tibia was not affected by the femoral fracture.", "contents": "Overgrowth of the femur after fracture of the shaft in childhood. Twenty-six children conservatively treated for fracture of the femoral shaft have been reviewed with regard to differences in limb length seven to ten years after the injury. In nine patients the clinical measurements were checked against radiographic measurements of both femora and tibiae. The following conclusions were reached. Nearly two-thirds of the patients had overgrowth of the femur of 10 millimetres or more. Shortening of 15 to 20 millimetres at the fracture site was well compensated for by accelerated growth. Growth acceleration seemed to take place during the healing period and the difference at the end of healing was permanent. Overgrowth was promoted by comminuted and long oblique fractures and by overriding of the fracture ends, but was not influenced by the age at fracture, the duration of treatment or the level of fracture of the shaft. Growth of the tibia was not affected by the femoral fracture.", "PMID": 956252}
{"id": "pubmed23n0032_2086", "title": "Parastremmatic dwarfism.", "content": "A girl aged ten, of Cape Coloured stock, with typical features of parastremmatic dwarfism has been investigated. The clinical manifestations included disproportionate dwarfism, limb deformity, a short stiff neck, and marked thoracic kyphosis. The radiographic changes were dramatic, the skeleton having a \"flocky\" appearance due to patches of radiolucency in an irregular lattice of sclerosis. The metaphyses and epiphyses of the long bones were grossly expanded, and the vertebrae were flattened and distorted. The clinical and radiographic features of five other previously reported individuals with parastremmatic dwarfism were very similar to those of our patient. The differential diagnosis of this condition includes metatrophic dwarfism, diatrophic dwarfism and the spondylo-epiphysial dysplasias. However, the unique \"flocky\" radiographic appearance of the bones permits diagnostic precision. There is some evidence to indicate that parastremmatic dwarfism might be transmitted as an autosomal dominant, although this is by no means certain. The fact that our patient had seven normal siblings and unaffected parents would be compatible woth autosomal recessive inheritance.", "contents": "Parastremmatic dwarfism. A girl aged ten, of Cape Coloured stock, with typical features of parastremmatic dwarfism has been investigated. The clinical manifestations included disproportionate dwarfism, limb deformity, a short stiff neck, and marked thoracic kyphosis. The radiographic changes were dramatic, the skeleton having a \"flocky\" appearance due to patches of radiolucency in an irregular lattice of sclerosis. The metaphyses and epiphyses of the long bones were grossly expanded, and the vertebrae were flattened and distorted. The clinical and radiographic features of five other previously reported individuals with parastremmatic dwarfism were very similar to those of our patient. The differential diagnosis of this condition includes metatrophic dwarfism, diatrophic dwarfism and the spondylo-epiphysial dysplasias. However, the unique \"flocky\" radiographic appearance of the bones permits diagnostic precision. There is some evidence to indicate that parastremmatic dwarfism might be transmitted as an autosomal dominant, although this is by no means certain. The fact that our patient had seven normal siblings and unaffected parents would be compatible woth autosomal recessive inheritance.", "PMID": 956253}
{"id": "pubmed23n0032_2087", "title": "Massive osteolysis complicated by chylothorax successfully treated by pleurodesis.", "content": "A rare complication of massive osteolysis (disappearing bones, Gorham's disease)is presented--namely involvement and invasion of the thoracic duct by the angiomatous tissue spreading locally from involved upper thoracic vertebrae. The resultant chylothorax in our patient was investigated and the site of the lymphatic fistula established by lymphography. Operative obliteration of the chylous leak was successfully achieved and the patient has survived. A review of the literature shows this complication to have been fatal in six other cases, including Gorham's first patient.", "contents": "Massive osteolysis complicated by chylothorax successfully treated by pleurodesis. A rare complication of massive osteolysis (disappearing bones, Gorham's disease)is presented--namely involvement and invasion of the thoracic duct by the angiomatous tissue spreading locally from involved upper thoracic vertebrae. The resultant chylothorax in our patient was investigated and the site of the lymphatic fistula established by lymphography. Operative obliteration of the chylous leak was successfully achieved and the patient has survived. A review of the literature shows this complication to have been fatal in six other cases, including Gorham's first patient.", "PMID": 956254}
{"id": "pubmed23n0032_2088", "title": "Madelung's disease of the wrist and dyschondrosteosis.", "content": "Twenty-six index patients with primary Madelung's deformity of the wrist were examined, as were sixty-five of their relatives. Standard radiographs were taken and measured to determine whether mesomelic dwarfism, a feature of dyschondrosteosis, was present. No case of mesomelia was found. No evidence of Madelung's deformity was found in the relatives of the index patients, who were all female. It is concluded that primary Madelung's deformity of the wrist is not an expressions of dyschondrosteosis, that it is not inherited, and that it does not occur in the male. One index patient with dyschondrosteosis was examined. Eight of her relatives were similarly affected, and three of these were male. It is suggested that previously recorded male cases of Madelung's deformity of the wrist were expressions of dyschondrosteosis or of secondary deformity.", "contents": "Madelung's disease of the wrist and dyschondrosteosis. Twenty-six index patients with primary Madelung's deformity of the wrist were examined, as were sixty-five of their relatives. Standard radiographs were taken and measured to determine whether mesomelic dwarfism, a feature of dyschondrosteosis, was present. No case of mesomelia was found. No evidence of Madelung's deformity was found in the relatives of the index patients, who were all female. It is concluded that primary Madelung's deformity of the wrist is not an expressions of dyschondrosteosis, that it is not inherited, and that it does not occur in the male. One index patient with dyschondrosteosis was examined. Eight of her relatives were similarly affected, and three of these were male. It is suggested that previously recorded male cases of Madelung's deformity of the wrist were expressions of dyschondrosteosis or of secondary deformity.", "PMID": 956255}
{"id": "pubmed23n0032_2089", "title": "A radiological sign of entrapment of the median nerve in the elbow joint after posterior dislocation. A report of two cases.", "content": "Two boys with entrapment of the median nerve in the elbow joint after closed reduction of a posterior dislocation with fracture of the medial epicondyle showed a characteristic radiological sign in the anteroposterior radiograph after two to three months. The sign was a depression in the cortex on the ulnar side of the distal humeral metaphysis, with interruption of the local periosteal reaction. At operation in both patients the depression was found to correspond with the place where the median nerve reached the posterior surface of the humerus. Radiographs taken after transverse section of the nerve above and below the joint capsule and end-to-end suture showed gradual disappearance of the cortical depression.", "contents": "A radiological sign of entrapment of the median nerve in the elbow joint after posterior dislocation. A report of two cases. Two boys with entrapment of the median nerve in the elbow joint after closed reduction of a posterior dislocation with fracture of the medial epicondyle showed a characteristic radiological sign in the anteroposterior radiograph after two to three months. The sign was a depression in the cortex on the ulnar side of the distal humeral metaphysis, with interruption of the local periosteal reaction. At operation in both patients the depression was found to correspond with the place where the median nerve reached the posterior surface of the humerus. Radiographs taken after transverse section of the nerve above and below the joint capsule and end-to-end suture showed gradual disappearance of the cortical depression.", "PMID": 956256}
{"id": "pubmed23n0032_2090", "title": "The control of pain after Keller's operation by the instillation of local anaesthetic before closure. A prospective controlled trial.", "content": "With the aim of controlling pain after Keller's operation, a solution of a long-acting local anaesthetic was instilled into the field before closure in a series of thirty patients. In a controlled prospective trial this simple technique by itself proved to be significantly better than either foot block at the ankle or the usual analgesics and was free from local or systemic complications.", "contents": "The control of pain after Keller's operation by the instillation of local anaesthetic before closure. A prospective controlled trial. With the aim of controlling pain after Keller's operation, a solution of a long-acting local anaesthetic was instilled into the field before closure in a series of thirty patients. In a controlled prospective trial this simple technique by itself proved to be significantly better than either foot block at the ankle or the usual analgesics and was free from local or systemic complications.", "PMID": 956257}
{"id": "pubmed23n0032_2091", "title": "Waldeyer's hernia.", "content": "One of the rare sites of internal abdominal hernia is Waldeyer's peritoneal fossa. A case report in a young adult male is presented with preoperative roentgen diagnosis on a small bowel follow-through. Anatomic types of paraduodenal fossae are described and some points of surgical importance are stressed.", "contents": "Waldeyer's hernia. One of the rare sites of internal abdominal hernia is Waldeyer's peritoneal fossa. A case report in a young adult male is presented with preoperative roentgen diagnosis on a small bowel follow-through. Anatomic types of paraduodenal fossae are described and some points of surgical importance are stressed.", "PMID": 956258}
{"id": "pubmed23n0032_2092", "title": "Venous intravasation of pantopaque during myelography. Report of two cases and a review of the literature.", "content": "Pantopaque venous intravasation, an uncommon complication of myelogram, has been reported since 1945. Traumatic spinal puncture has been known to be associated with Pantopaque venous intravasation, but its close relationship with the level of spinal puncture at the L5-S1 intervertebral disc space has not been emphasized. Spinal puncture at that level should be discouraged in order to prevent Pantopaque venous intravasation and pulmonary embolism during myelography.", "contents": "Venous intravasation of pantopaque during myelography. Report of two cases and a review of the literature. Pantopaque venous intravasation, an uncommon complication of myelogram, has been reported since 1945. Traumatic spinal puncture has been known to be associated with Pantopaque venous intravasation, but its close relationship with the level of spinal puncture at the L5-S1 intervertebral disc space has not been emphasized. Spinal puncture at that level should be discouraged in order to prevent Pantopaque venous intravasation and pulmonary embolism during myelography.", "PMID": 956259}
{"id": "pubmed23n0032_2093", "title": "Gastric leiomyoblastoma in a child.", "content": "A gastric leiomyoblastoma can occur in any age group, but the prognosis and sex distribution are different in the child-adolescent group than in adults. In the adult, the lesion is generally considered to be benign, though in the child-adolescent group it may metastasize to regional lymph nodes. The possibility of a leiomyoblastoma is suggested by multicentric intramural gastric tumors discovered in a female child or adolescent who has had gastrointestinal hemorrhage.", "contents": "Gastric leiomyoblastoma in a child. A gastric leiomyoblastoma can occur in any age group, but the prognosis and sex distribution are different in the child-adolescent group than in adults. In the adult, the lesion is generally considered to be benign, though in the child-adolescent group it may metastasize to regional lymph nodes. The possibility of a leiomyoblastoma is suggested by multicentric intramural gastric tumors discovered in a female child or adolescent who has had gastrointestinal hemorrhage.", "PMID": 956260}
{"id": "pubmed23n0032_2094", "title": "Spontaneous nephro-duodenal fistula.", "content": "Nephroduodenal fistulas are an uncommon clinical entity characterized by upper abdominal pain and associated with pyelonephritis and renal calculus formation. Most of the recent cases reported are secondary to inflammatory changes in the kidney. Two cases are reported here, one of a nephroduodenal fistula and another of a combined nephroduodenal and nephrocolic fistulae. Carefully monitored X-ray studies determined in both cases the exact site and extent of the fistulae. The optimal treatment is nephrectomy.", "contents": "Spontaneous nephro-duodenal fistula. Nephroduodenal fistulas are an uncommon clinical entity characterized by upper abdominal pain and associated with pyelonephritis and renal calculus formation. Most of the recent cases reported are secondary to inflammatory changes in the kidney. Two cases are reported here, one of a nephroduodenal fistula and another of a combined nephroduodenal and nephrocolic fistulae. Carefully monitored X-ray studies determined in both cases the exact site and extent of the fistulae. The optimal treatment is nephrectomy.", "PMID": 956261}
{"id": "pubmed23n0032_2095", "title": "Resident evaluation - is our method effective?", "content": "In an earlier paper, \"Resident Evaluation--Is Your Method Effective?\", it was emphasized that the objectives for the residency program in Radiology should be clearly delineated and used to judge the performance of the resident in all stages of the training program. After having had approximately three years' experience in the use of the procedures outlined in the original paper, we are recommending some changes and modifications. The purpose of the evaluation is to provide clear and detailed evidence of the performance of the resident over the period of his training program. As outlined in the previous paper, the basic principles -- learning is a very individual matter, learning depends on the motivation of the individual, and learning depends on the satisfaction in the learning task--remain unaltered. Changes have been made in the design, execution and details of evaluation. The final sucess of any training program will necessarily be measured by the degree to which the residents attain the outlined objectives of the program and the degree to which the graduates of the program are accepted into the specialty by the accrediting bodies.", "contents": "Resident evaluation - is our method effective? In an earlier paper, \"Resident Evaluation--Is Your Method Effective?\", it was emphasized that the objectives for the residency program in Radiology should be clearly delineated and used to judge the performance of the resident in all stages of the training program. After having had approximately three years' experience in the use of the procedures outlined in the original paper, we are recommending some changes and modifications. The purpose of the evaluation is to provide clear and detailed evidence of the performance of the resident over the period of his training program. As outlined in the previous paper, the basic principles -- learning is a very individual matter, learning depends on the motivation of the individual, and learning depends on the satisfaction in the learning task--remain unaltered. Changes have been made in the design, execution and details of evaluation. The final sucess of any training program will necessarily be measured by the degree to which the residents attain the outlined objectives of the program and the degree to which the graduates of the program are accepted into the specialty by the accrediting bodies.", "PMID": 956262}
{"id": "pubmed23n0032_2096", "title": "Half-body radiotherapy of advanced cancer.", "content": "Half-body radiotherapy to deliver doses up to 1000 rads in a single exposure evolved to meet a need in treating patients with advanced cancer. Large fields are required and a high radiation output. Treatments are well tolerated, although radiation sickness occurs in 80% of upper half and 33% of lower half-body treatments. Marrow depression is not a problem and the second half body can be treated when the peripheral blood count has returned to normal. Subjective improvement occurs in most patients and this group had a median survival of six months.", "contents": "Half-body radiotherapy of advanced cancer. Half-body radiotherapy to deliver doses up to 1000 rads in a single exposure evolved to meet a need in treating patients with advanced cancer. Large fields are required and a high radiation output. Treatments are well tolerated, although radiation sickness occurs in 80% of upper half and 33% of lower half-body treatments. Marrow depression is not a problem and the second half body can be treated when the peripheral blood count has returned to normal. Subjective improvement occurs in most patients and this group had a median survival of six months.", "PMID": 956263}
{"id": "pubmed23n0032_2097", "title": "Localization of gallium-67 citrate in salivary glands following radiation therapy.", "content": "Gallium-67 citrate was found to localize in the salivary glands of eight patients after therapeutic doses of radiation to a treatment field including the glands. In two patients, autopsy evidence to the effect that no primary disease was present to explain such uptake was obtained. In another two patients the abnormal uptake was not observed on follow-up. The recognition that radiation sialadenitis is a potential cause of false-positive gallium scintiscans is important in their interpretation.", "contents": "Localization of gallium-67 citrate in salivary glands following radiation therapy. Gallium-67 citrate was found to localize in the salivary glands of eight patients after therapeutic doses of radiation to a treatment field including the glands. In two patients, autopsy evidence to the effect that no primary disease was present to explain such uptake was obtained. In another two patients the abnormal uptake was not observed on follow-up. The recognition that radiation sialadenitis is a potential cause of false-positive gallium scintiscans is important in their interpretation.", "PMID": 956264}
{"id": "pubmed23n0032_2098", "title": "Ataxia-telangiectasia.", "content": "The clinical and radiographic features of twenty-two cases of ataxia-telangiectasia are reviewed. Severity of pulmonary manifestations tended to correlate closely with severity of immunodeficiency. Observations are made upon the problem of intercurrent malignancy in ataxia-telangiectasia. The radiographic studies of patients seen at the National Institutes of Health with A-T were reviewed retrospectively and correlated with degrees of immunodeficiency determined clinically. Three fairly distinct groups with high, intermediate and low incidence of sinopulmonary disease were found. Comment is made on the problem of increased malignancy in A-T and possible clues in the detection by the radiologist.", "contents": "Ataxia-telangiectasia. The clinical and radiographic features of twenty-two cases of ataxia-telangiectasia are reviewed. Severity of pulmonary manifestations tended to correlate closely with severity of immunodeficiency. Observations are made upon the problem of intercurrent malignancy in ataxia-telangiectasia. The radiographic studies of patients seen at the National Institutes of Health with A-T were reviewed retrospectively and correlated with degrees of immunodeficiency determined clinically. Three fairly distinct groups with high, intermediate and low incidence of sinopulmonary disease were found. Comment is made on the problem of increased malignancy in A-T and possible clues in the detection by the radiologist.", "PMID": 956265}
{"id": "pubmed23n0032_2099", "title": "Angiographic demonstration of a thoracic aortic aneurysm with rupture into the pulmonary artery.", "content": "A case of rupture of a thoracic aortic aneurysm into the left pulmonary artery is presented. This is an unusual complication of an aortic aneurysm. The importance of angiography in its accurate and early diagnosis is described.", "contents": "Angiographic demonstration of a thoracic aortic aneurysm with rupture into the pulmonary artery. A case of rupture of a thoracic aortic aneurysm into the left pulmonary artery is presented. This is an unusual complication of an aortic aneurysm. The importance of angiography in its accurate and early diagnosis is described.", "PMID": 956266}
{"id": "pubmed23n0032_2100", "title": "Roentgen features of the rheumatoid mid- and hindfoot.", "content": "The roentgen features of rheumatoid involvement of the mid- and hindfoot are analyzed utilizing anatomic and pathologic material from \"control\" and rheumatoid cadavers and a detailed radiographic study of a rheumatoid population. Characteristic findings include diffuse abnormalities predilecting the calcaneus, talocalcaneonavicular and tarsometatarsal joints with joint space narrowing, bony fusion and reactive sclerosis. Rheumatoid localization in the mid- and hindfoot is not unusual, particularly in long-standing disease, and is almost universally associated with metatarsophalangeal alterations.", "contents": "Roentgen features of the rheumatoid mid- and hindfoot. The roentgen features of rheumatoid involvement of the mid- and hindfoot are analyzed utilizing anatomic and pathologic material from \"control\" and rheumatoid cadavers and a detailed radiographic study of a rheumatoid population. Characteristic findings include diffuse abnormalities predilecting the calcaneus, talocalcaneonavicular and tarsometatarsal joints with joint space narrowing, bony fusion and reactive sclerosis. Rheumatoid localization in the mid- and hindfoot is not unusual, particularly in long-standing disease, and is almost universally associated with metatarsophalangeal alterations.", "PMID": 956267}
{"id": "pubmed23n0032_2101", "title": "Lipid vesicle-cell interactions. I. Hemagglutination and hemolysis.", "content": "The interaction of lipid vesicles (liposomes) of several different compositions with erythrocytes has been investigated. Lecithin liposomes, rendered positively charged with stearylamine, exhibit potent hemagglutination activity in media containing low concentrations of electrolytes. The hemagglutination titer is found to be a linear function of the zeta potential of the lipid vesicles. Hemagglutination is reduced when the surface potential of the cells is made more positive by pH adjustment or enzyme treatment. Similarly, hemagglutination is reduced by increasing concentrations of electrolytes. Hemagglutination is examined theoretically and is shown to be consistent with vesicle-cell interactions that are due to only electrostatic forces. Vesicles containing lysolecithin in addition to lecithin and stearylamine cause lysis of erythrocytes, provided the lipids of the vesicles are above the crystal-liquid crystal phase transition temperature. In addition, hemolysis requires close juxtaposition of the vesicle to the cell membrane; vesicles precoated with antibodies exhibit severely diminished hemolytic activities, only a small fraction of which can be attributed to a reduction in hemagglutination titer. Evidence is presented indicating that a single vesicle is sufficient to lyse one cell. With regard to hemagglutination and hemolysis, lipid vesicles of simple composition mimic paramyxoviruses such as Sendai virus.", "contents": "Lipid vesicle-cell interactions. I. Hemagglutination and hemolysis. The interaction of lipid vesicles (liposomes) of several different compositions with erythrocytes has been investigated. Lecithin liposomes, rendered positively charged with stearylamine, exhibit potent hemagglutination activity in media containing low concentrations of electrolytes. The hemagglutination titer is found to be a linear function of the zeta potential of the lipid vesicles. Hemagglutination is reduced when the surface potential of the cells is made more positive by pH adjustment or enzyme treatment. Similarly, hemagglutination is reduced by increasing concentrations of electrolytes. Hemagglutination is examined theoretically and is shown to be consistent with vesicle-cell interactions that are due to only electrostatic forces. Vesicles containing lysolecithin in addition to lecithin and stearylamine cause lysis of erythrocytes, provided the lipids of the vesicles are above the crystal-liquid crystal phase transition temperature. In addition, hemolysis requires close juxtaposition of the vesicle to the cell membrane; vesicles precoated with antibodies exhibit severely diminished hemolytic activities, only a small fraction of which can be attributed to a reduction in hemagglutination titer. Evidence is presented indicating that a single vesicle is sufficient to lyse one cell. With regard to hemagglutination and hemolysis, lipid vesicles of simple composition mimic paramyxoviruses such as Sendai virus.", "PMID": 956268}
{"id": "pubmed23n0032_2102", "title": "Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var. Vignae.", "content": "Aspects of the ultrastructure of mitotic nuclei of the fungus Uromyces phaseoli var. vignae are described from both intercellular hyphae in the cowpea host and infection structures induced to differentiate in vitro. The interphase nucleus-associated organelle (NAO) consists of two trilamellar acircular disks connceted by an osmiophilic bar. The intranuclear spindle develops between these disks when they separate. The spindle contains pole to pole, interdigitating, chromosomal, and fragmentary microtubules arranged to form a central bundle along the surface of which lie the metaphase chromosomes. No metaphase plate is found. There are up to three microtubules per kinetochore and approximately 14 chromosomes on the haploid spindle. Telophase elongation appears to involve extension of pole to pole microtubules with no evidence for the remaining presence of interdigitating microtubules. Concomitantly, numerous cytoplasmic microtubules develop from each NAO disk where few or none are present in other phases. Reformation of the interphase NAO involves the formation of a sausage-shaped intermediate at late telophase. The nuclear envelope remains intact and the nucleolus persists throughtout division. Various aspects of the spindle and NAOs appear to be evolutionary intermediates between Ascomycetes and higher Basidiomycetes, thus supporting the theory of Basidiomycete evolution from the former group and demonstrating an encouraging correlation between mitotic characteristics and other phylogenetic markers.", "contents": "Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var. Vignae. Aspects of the ultrastructure of mitotic nuclei of the fungus Uromyces phaseoli var. vignae are described from both intercellular hyphae in the cowpea host and infection structures induced to differentiate in vitro. The interphase nucleus-associated organelle (NAO) consists of two trilamellar acircular disks connceted by an osmiophilic bar. The intranuclear spindle develops between these disks when they separate. The spindle contains pole to pole, interdigitating, chromosomal, and fragmentary microtubules arranged to form a central bundle along the surface of which lie the metaphase chromosomes. No metaphase plate is found. There are up to three microtubules per kinetochore and approximately 14 chromosomes on the haploid spindle. Telophase elongation appears to involve extension of pole to pole microtubules with no evidence for the remaining presence of interdigitating microtubules. Concomitantly, numerous cytoplasmic microtubules develop from each NAO disk where few or none are present in other phases. Reformation of the interphase NAO involves the formation of a sausage-shaped intermediate at late telophase. The nuclear envelope remains intact and the nucleolus persists throughtout division. Various aspects of the spindle and NAOs appear to be evolutionary intermediates between Ascomycetes and higher Basidiomycetes, thus supporting the theory of Basidiomycete evolution from the former group and demonstrating an encouraging correlation between mitotic characteristics and other phylogenetic markers.", "PMID": 956269}
{"id": "pubmed23n0032_2103", "title": "Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. II. Cytochemical localization within Golgi and ER membranes.", "content": "The presence of adenylate cyclase (AC) in liver Golgi and microsomal fractions from ethanol-treated rats was tested cytochemically using 5'-adenylyl imidodiphosphate (AMP-PNP) lead phosphate method. Parallel biochemical assays showed that rat liver Golgi AC was only partially inhibited by lead: in the presence of 1 mM Pb++ 80% of the enzyme was preserved, while when 2 mM Pb++ was used 25% remained. No cAMP was formed when the AMP-PNP medium was incubated in the presence of 1 or 2 mM Pb++ but in the absence of cell fractions, indicating that at these concentrations Pb++ does not cause the nonenzymatic hydrolysis of AMP-PNP. Therefore, the reaction product observed by cytochemical localization is not due to the nonenzymatic hydrolysis of AMP-PNP by Pb++. In Golgi subfractions, lead phosphate reaction product was widely distributed among Golgi elements: it was seen in association with the majority of the very low density lipoprotein-filled secretory droplets which predominated in the two lightest Golgi fractions (GF1 and GF2) as well as within the majority of the cisternae found in the heaviest Golgi fraction (GF3). In the latter, reaction product was heaviest along the dilated peripheral rims of the cisternae. In all cases, the reaction product was localized to the outside or cytoplasmic face of the Golgi membranes. When microsomes were incubated cytochemically for AC, deposits were found on the cytoplasmic surface of smooth endoplasmic reticulum (ER) membranes, but none were observed on rough ER membranes. The results confirm the biochemical data reported previously indicating the presence of AC in Golgi and smooth microsomal fractions from rat liver and further demonstrate that the activity is indeed indigenous to Golgi elements and not due to plasma membrane contaminants. They also indicate that AC is widely distributed among Golgi and smooth ER elements. Thus, AC is not restricted in its distribution to plasma membranes as usually assumed.", "contents": "Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. II. Cytochemical localization within Golgi and ER membranes. The presence of adenylate cyclase (AC) in liver Golgi and microsomal fractions from ethanol-treated rats was tested cytochemically using 5'-adenylyl imidodiphosphate (AMP-PNP) lead phosphate method. Parallel biochemical assays showed that rat liver Golgi AC was only partially inhibited by lead: in the presence of 1 mM Pb++ 80% of the enzyme was preserved, while when 2 mM Pb++ was used 25% remained. No cAMP was formed when the AMP-PNP medium was incubated in the presence of 1 or 2 mM Pb++ but in the absence of cell fractions, indicating that at these concentrations Pb++ does not cause the nonenzymatic hydrolysis of AMP-PNP. Therefore, the reaction product observed by cytochemical localization is not due to the nonenzymatic hydrolysis of AMP-PNP by Pb++. In Golgi subfractions, lead phosphate reaction product was widely distributed among Golgi elements: it was seen in association with the majority of the very low density lipoprotein-filled secretory droplets which predominated in the two lightest Golgi fractions (GF1 and GF2) as well as within the majority of the cisternae found in the heaviest Golgi fraction (GF3). In the latter, reaction product was heaviest along the dilated peripheral rims of the cisternae. In all cases, the reaction product was localized to the outside or cytoplasmic face of the Golgi membranes. When microsomes were incubated cytochemically for AC, deposits were found on the cytoplasmic surface of smooth endoplasmic reticulum (ER) membranes, but none were observed on rough ER membranes. The results confirm the biochemical data reported previously indicating the presence of AC in Golgi and smooth microsomal fractions from rat liver and further demonstrate that the activity is indeed indigenous to Golgi elements and not due to plasma membrane contaminants. They also indicate that AC is widely distributed among Golgi and smooth ER elements. Thus, AC is not restricted in its distribution to plasma membranes as usually assumed.", "PMID": 956270}
{"id": "pubmed23n0032_2104", "title": "Exocytosis in secretory cells of rat lacrimal gland. Peroxidase release from lobules and isolated cells upon cholinergic stimulation.", "content": "Release of peroxidase from secretory cells of rat lacrimal gland upon cholinergic stimulation was studied in vitro with single lobules and isolated cells (lacrimocytes). Isolated lobules, kept in Eagle's medium, remain structurally intact and reaction product of peroxidase is confined to cisternae of rough endoplasmic reticulum, elements of the Golgi apparatus, and all secretory granules. Morphologically, exocytosis occurs by membrane fusion and discharge of granule content. The highest rate of peroxidase released from lobules is observed at 10(-4) M carbamylcholine. The specific activity of peroxidase released into the medium is fourfold higher as compared to the lobules. Release of peroxidase is suppressed by atropine when added before or after the addition of carbamylcholine. At 4 degrees C, no peroxidase release occurs upon cholinergic stimulation. The exocytotic release of peroxidase is dependent on energy supply, as indicated by substantial inhibition (at 37 degrees C) under anoxic conditions or in the presence of dinitrophenol, KCN, or carboxyatractyloside. Furthermore, the process is sensitive to colchicine and vinblastine. Isolated lacrimocytes, consiting of 95% secretory acinar cells, are prepared by digestion with collagenase, hyaluronidase, and trypsin. They retain the characteristic polarity of secretory cells in situ, and localization of peroxidase is the same as in lobules. Since isolated lacrimocytes respond to cholinergic stimulation in the same way as lobules, the receptors are not damaged by the isolation procedure and appear to be associated directly with the exocrine cell. Oxygen uptake by isolated lacrimocytes is about 14 nmol O2 X min-1 X 10(-6) cells; it is about doubled by uncoupling with dinitrophenol. Oxygen uptake rises by 20-30% above the resting rate upon cholinergic stimulation. This additional uptake is suppressed by atropine or by added cholinesterase, indicating that continuous receptor occupancy may be required for the energy demand by exocytosis. On the basis of the specific activity of peroxidase in the medium, the energy demand resulting from cholinergic stimulation is estimated to be 0.08 mumol ATP (or energy-rich phosphate bonds) per microgram of protein released from the lacrimocytes.", "contents": "Exocytosis in secretory cells of rat lacrimal gland. Peroxidase release from lobules and isolated cells upon cholinergic stimulation. Release of peroxidase from secretory cells of rat lacrimal gland upon cholinergic stimulation was studied in vitro with single lobules and isolated cells (lacrimocytes). Isolated lobules, kept in Eagle's medium, remain structurally intact and reaction product of peroxidase is confined to cisternae of rough endoplasmic reticulum, elements of the Golgi apparatus, and all secretory granules. Morphologically, exocytosis occurs by membrane fusion and discharge of granule content. The highest rate of peroxidase released from lobules is observed at 10(-4) M carbamylcholine. The specific activity of peroxidase released into the medium is fourfold higher as compared to the lobules. Release of peroxidase is suppressed by atropine when added before or after the addition of carbamylcholine. At 4 degrees C, no peroxidase release occurs upon cholinergic stimulation. The exocytotic release of peroxidase is dependent on energy supply, as indicated by substantial inhibition (at 37 degrees C) under anoxic conditions or in the presence of dinitrophenol, KCN, or carboxyatractyloside. Furthermore, the process is sensitive to colchicine and vinblastine. Isolated lacrimocytes, consiting of 95% secretory acinar cells, are prepared by digestion with collagenase, hyaluronidase, and trypsin. They retain the characteristic polarity of secretory cells in situ, and localization of peroxidase is the same as in lobules. Since isolated lacrimocytes respond to cholinergic stimulation in the same way as lobules, the receptors are not damaged by the isolation procedure and appear to be associated directly with the exocrine cell. Oxygen uptake by isolated lacrimocytes is about 14 nmol O2 X min-1 X 10(-6) cells; it is about doubled by uncoupling with dinitrophenol. Oxygen uptake rises by 20-30% above the resting rate upon cholinergic stimulation. This additional uptake is suppressed by atropine or by added cholinesterase, indicating that continuous receptor occupancy may be required for the energy demand by exocytosis. On the basis of the specific activity of peroxidase in the medium, the energy demand resulting from cholinergic stimulation is estimated to be 0.08 mumol ATP (or energy-rich phosphate bonds) per microgram of protein released from the lacrimocytes.", "PMID": 956271}
{"id": "pubmed23n0032_2105", "title": "Initial attachment of baby hamster kidney cells to an epoxy substratum. Ultrastructural analysis.", "content": "In the presence of serum-containing medium, BHK cells attached and spread during a 1-h period onto a 3-5 nm thick serum layer absorbed on the substratum surface. The closest approach of the plasma membrane to the serum layer was observed to be about 9nm, which was determined by tilting the sectioned cells in a goniometer holder. Bundles of microfilaments or other cytoplasmic specializations were not observed in association with the regions of close contact. However, in the space between the plasma membrane and the adsorbed serum layer, a diffusely stained material could be visualized after fixation/staining by the tannic acid-glutaraldehyde technique. This technique also permitted increased clarity of visualization of trilaminar appearance of the plasma membrane. The distribution and mobility of anionic sites on the surfaces of attached and spreading cells was determined by labeling with polycationic ferritin. We observed movement of polycationic ferritin into large clusters on the cell surface, collapse of cell surface microextensions, and endocytosis, all of which were similar to our previous findings utilizing cells in suspension. However, the absolute amount of ferritin bound to the upper cell surface was less than that previously observed when suspended cells were put under similar labeling conditions. Also, polycationic ferritin did not appear to penetrate between the lower cell surface and the substratum.", "contents": "Initial attachment of baby hamster kidney cells to an epoxy substratum. Ultrastructural analysis. In the presence of serum-containing medium, BHK cells attached and spread during a 1-h period onto a 3-5 nm thick serum layer absorbed on the substratum surface. The closest approach of the plasma membrane to the serum layer was observed to be about 9nm, which was determined by tilting the sectioned cells in a goniometer holder. Bundles of microfilaments or other cytoplasmic specializations were not observed in association with the regions of close contact. However, in the space between the plasma membrane and the adsorbed serum layer, a diffusely stained material could be visualized after fixation/staining by the tannic acid-glutaraldehyde technique. This technique also permitted increased clarity of visualization of trilaminar appearance of the plasma membrane. The distribution and mobility of anionic sites on the surfaces of attached and spreading cells was determined by labeling with polycationic ferritin. We observed movement of polycationic ferritin into large clusters on the cell surface, collapse of cell surface microextensions, and endocytosis, all of which were similar to our previous findings utilizing cells in suspension. However, the absolute amount of ferritin bound to the upper cell surface was less than that previously observed when suspended cells were put under similar labeling conditions. Also, polycationic ferritin did not appear to penetrate between the lower cell surface and the substratum.", "PMID": 956272}
{"id": "pubmed23n0032_2106", "title": "Transport and countertransport of thymidine in ATP depleted and thymidine kinase-deficient Novikoff rat hepatoma and mouse L cells: evidence of a high Km facilitated diffusion system with wide nucleoside specificity.", "content": "Incubation of cultured Novikoff rat hepatoma and mouse L cells in a glucose-free basal medium containing 5 mM KCN and 5 mM iodoacetate for about 10 minutes resulted in a complete depletion of the cells of ATP. ATP-depleted wild type cells or thymidine kinase-deficient sublines of Novikoff or L cells took up thymidine rapidly from the medium without concentrating it intracellularly, and exhibited countertransport of thymidine. Thus uptake was by facilitated diffusion. This transport system differs from the substrate-specific, low-Km (0.5 muM] thymidine transport system previously described for various types of cultured cells in that it exhibits an at least 100-fold higher Km and transports equally well various ribo- and deoxyribonucleosides. The results suggest that the rate-limiting step in thymidine incorporation into the nucleotide pool by wild type cells is phosphorylation rather than transport, or that the cells possess two transport systems, a facilitated diffusion system with low substrate specificity and a second system which involves substrate phosphorylation by thymidine kinase.", "contents": "Transport and countertransport of thymidine in ATP depleted and thymidine kinase-deficient Novikoff rat hepatoma and mouse L cells: evidence of a high Km facilitated diffusion system with wide nucleoside specificity. Incubation of cultured Novikoff rat hepatoma and mouse L cells in a glucose-free basal medium containing 5 mM KCN and 5 mM iodoacetate for about 10 minutes resulted in a complete depletion of the cells of ATP. ATP-depleted wild type cells or thymidine kinase-deficient sublines of Novikoff or L cells took up thymidine rapidly from the medium without concentrating it intracellularly, and exhibited countertransport of thymidine. Thus uptake was by facilitated diffusion. This transport system differs from the substrate-specific, low-Km (0.5 muM] thymidine transport system previously described for various types of cultured cells in that it exhibits an at least 100-fold higher Km and transports equally well various ribo- and deoxyribonucleosides. The results suggest that the rate-limiting step in thymidine incorporation into the nucleotide pool by wild type cells is phosphorylation rather than transport, or that the cells possess two transport systems, a facilitated diffusion system with low substrate specificity and a second system which involves substrate phosphorylation by thymidine kinase.", "PMID": 956273}
{"id": "pubmed23n0032_2107", "title": "Density regulation of amino acid transport in cultured, androgen-responsive tumor cells.", "content": "In androgen-responsive cells, activity of a sodium-dependent transport system for neutral amino acids is shown to be density-dependent, whereas activity of a sodium-independent system is not.  Transport of the non-metabolizable amino acids, alpha-aminoisobutyric acid )alpha-aib) was largely sodium-dependent, wherease transport of cycloleucine (cyleu) was by  both sodium-dependent and sodium-independent systems.  The km for cyleu transport changed with density because only the sodium-dependent portion of this transport was density-dependent.  By contrast, the Vmax for alpha-aib transport changed with density, indicating that either amount of functional membrane carrier of the activity of the sodium pump was affected.", "contents": "Density regulation of amino acid transport in cultured, androgen-responsive tumor cells. In androgen-responsive cells, activity of a sodium-dependent transport system for neutral amino acids is shown to be density-dependent, whereas activity of a sodium-independent system is not.  Transport of the non-metabolizable amino acids, alpha-aminoisobutyric acid )alpha-aib) was largely sodium-dependent, wherease transport of cycloleucine (cyleu) was by  both sodium-dependent and sodium-independent systems.  The km for cyleu transport changed with density because only the sodium-dependent portion of this transport was density-dependent.  By contrast, the Vmax for alpha-aib transport changed with density, indicating that either amount of functional membrane carrier of the activity of the sodium pump was affected.", "PMID": 956274}
{"id": "pubmed23n0032_2108", "title": "Density regulation of amino acid transport in cultured, androgen-responsive tumour cells.", "content": "In androgen-responsive cells, activity of a sodium-dependent transport system for neutral amino acids is shown to be density-dependent, whereas activity of a sodium-independent system is not. Transport of the non-metabolizable amino acid, alpha-aminoisobutyric acid (alpha-aib) was largely sodium-dependent, whereas transport of cycloleucine (cyleu) was by both sodium-dependent and sodium-independent systems. The Km for cyleu transport changed with density because only the sodium-dependent portion of this transport was density-dependent. By contrast, the Vmax for alpha-aib transport changed with density, indicating that either the amount of functional membrane carrier or the activity of the sodium pump was affected.", "contents": "Density regulation of amino acid transport in cultured, androgen-responsive tumour cells. In androgen-responsive cells, activity of a sodium-dependent transport system for neutral amino acids is shown to be density-dependent, whereas activity of a sodium-independent system is not. Transport of the non-metabolizable amino acid, alpha-aminoisobutyric acid (alpha-aib) was largely sodium-dependent, whereas transport of cycloleucine (cyleu) was by both sodium-dependent and sodium-independent systems. The Km for cyleu transport changed with density because only the sodium-dependent portion of this transport was density-dependent. By contrast, the Vmax for alpha-aib transport changed with density, indicating that either the amount of functional membrane carrier or the activity of the sodium pump was affected.", "PMID": 956275}
{"id": "pubmed23n0032_2109", "title": "Density-dependent regulation of proliferation rate in cultured, androgen-responsive, tumour cells.", "content": "Short-term cultures of androgen-responsive Shionogi 115 (S115) cells exhibited density-dependent regulation of proliferation rate in the presence or absence of testosterone. The average surface area per cell exposed to the growth medium was inversely proportional to population density. By contrast, long-term cultures (serially passaged in testosterone-containing medium for several months) did not exhibit density-dependent regulation of proliferation rate when grown in testosterone-containing medium. In this medium, cells became elongated and no longer exhibited any obvious decrease in exposed surface area with increasing density. Nevertheless, when subcultured into testosterone-free medium, these cells reverted to an epithelial morphology and exhibited density-dependent regulation of proliferation rate. These relationships suggested that the proliferation rate of cells decreased with density in proportion to the decrease in exposed surface area...", "contents": "Density-dependent regulation of proliferation rate in cultured, androgen-responsive, tumour cells. Short-term cultures of androgen-responsive Shionogi 115 (S115) cells exhibited density-dependent regulation of proliferation rate in the presence or absence of testosterone. The average surface area per cell exposed to the growth medium was inversely proportional to population density. By contrast, long-term cultures (serially passaged in testosterone-containing medium for several months) did not exhibit density-dependent regulation of proliferation rate when grown in testosterone-containing medium. In this medium, cells became elongated and no longer exhibited any obvious decrease in exposed surface area with increasing density. Nevertheless, when subcultured into testosterone-free medium, these cells reverted to an epithelial morphology and exhibited density-dependent regulation of proliferation rate. These relationships suggested that the proliferation rate of cells decreased with density in proportion to the decrease in exposed surface area...", "PMID": 956276}
{"id": "pubmed23n0032_2110", "title": "Cell cycle dependent changes in potassium transport.", "content": "K transport has been investigated during progression of cultured Ehrlich ascites tumor cells through the cell cycle. Using a double thymidine block technique, Ehrlich cells carried in continuous culture have been synchronized, as verified by simultaneous monitoring of cell number, cell volume, 3H-thymidine incorporation and mitotic index. Unidirectional influx, efflux and cell content of K have been monitored throughout the cell cycle. The nature of the pump mediated, ouabain-sensitive K flux and the furosemide-sensitive component of K flux, presumably representing K-K exchange, have also been evaluated. In early S period the ouabain sensitive component, representing the Na-K pump, comprises 52% of the total unidirectional K influx and subsequently declines during G2 period to a minimum of 40% in mid G2. During M and early S the activity again rises. As the ouabain sensitive component becomes maximal in late S period, the furosemide sensitive component diminishes from approximately 30% of the total influx to approximately 10%. The same pattern is observed in the G2 period. As the pump component diminishes, the furosemide sensitive component increases. Furosemide sensitive K efflux has also been monitored and the pattern is equivalent to that observed in the invlux studies. No change in net K flux is observed in the presence of furosemide. This indicates that the furosemide sensitive component represents an exchange component for K. These results are consistent with the conclusion that the alterations in exchange and pump fluxes are physiological events associated with progression of the cell cycle.", "contents": "Cell cycle dependent changes in potassium transport. K transport has been investigated during progression of cultured Ehrlich ascites tumor cells through the cell cycle. Using a double thymidine block technique, Ehrlich cells carried in continuous culture have been synchronized, as verified by simultaneous monitoring of cell number, cell volume, 3H-thymidine incorporation and mitotic index. Unidirectional influx, efflux and cell content of K have been monitored throughout the cell cycle. The nature of the pump mediated, ouabain-sensitive K flux and the furosemide-sensitive component of K flux, presumably representing K-K exchange, have also been evaluated. In early S period the ouabain sensitive component, representing the Na-K pump, comprises 52% of the total unidirectional K influx and subsequently declines during G2 period to a minimum of 40% in mid G2. During M and early S the activity again rises. As the ouabain sensitive component becomes maximal in late S period, the furosemide sensitive component diminishes from approximately 30% of the total influx to approximately 10%. The same pattern is observed in the G2 period. As the pump component diminishes, the furosemide sensitive component increases. Furosemide sensitive K efflux has also been monitored and the pattern is equivalent to that observed in the invlux studies. No change in net K flux is observed in the presence of furosemide. This indicates that the furosemide sensitive component represents an exchange component for K. These results are consistent with the conclusion that the alterations in exchange and pump fluxes are physiological events associated with progression of the cell cycle.", "PMID": 956277}
{"id": "pubmed23n0032_2111", "title": "Reversible independent alterations in glucose transport and metabolism in cultured human cells deprived of glucose.", "content": "We have measured uptake of 3H-hexoses into diploid human cells by exposing them to brief pulses of isotopic sugar during the log-growth, subconfluent-growth, and confluent-growth (contact inhibited) phases of the strain HSWP derived from human skin. 3H-deoxyglucose appears to be taken up three times faster than 3H-glucose. After exposure to 3H-glucose for longer than one minute, the cells excrete approximately 70% of the isotope into the medium as lactate. If lactate production (and hence excretion) is abolished by treating the cellls with iodoacetic acid or dinitrofluorobenzene, neither of which inhibits transport, the uptake of 3H-glucose is found to be in fact somewhat larger than that of 3H-deoxyglucose. If cells are deprived of glucose for 24 hours, apparent uptake of 3H-glucose is enhanced 10-fold or more. This latter increase is accounted for by 2- to 3-fold enhancement of true transport plus retention of greater than 90% of the radioactivity, since little lactate is formed or excreted in glucose-deprived cells. Deoxyglucose, galactose, or pyruvate when present during glucose deprivation each have quantitatively different effects on the cells' capacity to produce lactate from a short pulse of glucose, but none of them prevents the enhancement of hexose transport. After restoration of 5 mM glucose to starved cells, their metabolsim returns to normal (in the sense that approximately 70% of the glucose taken up in a pulse is again excreted as lactate), with a half-time of 0.5 hour; but the transport of hexoses returns to control levels much more slowly, with a half-time of approximately 6 hours. The two processes appear to be independently regulated.", "contents": "Reversible independent alterations in glucose transport and metabolism in cultured human cells deprived of glucose. We have measured uptake of 3H-hexoses into diploid human cells by exposing them to brief pulses of isotopic sugar during the log-growth, subconfluent-growth, and confluent-growth (contact inhibited) phases of the strain HSWP derived from human skin. 3H-deoxyglucose appears to be taken up three times faster than 3H-glucose. After exposure to 3H-glucose for longer than one minute, the cells excrete approximately 70% of the isotope into the medium as lactate. If lactate production (and hence excretion) is abolished by treating the cellls with iodoacetic acid or dinitrofluorobenzene, neither of which inhibits transport, the uptake of 3H-glucose is found to be in fact somewhat larger than that of 3H-deoxyglucose. If cells are deprived of glucose for 24 hours, apparent uptake of 3H-glucose is enhanced 10-fold or more. This latter increase is accounted for by 2- to 3-fold enhancement of true transport plus retention of greater than 90% of the radioactivity, since little lactate is formed or excreted in glucose-deprived cells. Deoxyglucose, galactose, or pyruvate when present during glucose deprivation each have quantitatively different effects on the cells' capacity to produce lactate from a short pulse of glucose, but none of them prevents the enhancement of hexose transport. After restoration of 5 mM glucose to starved cells, their metabolsim returns to normal (in the sense that approximately 70% of the glucose taken up in a pulse is again excreted as lactate), with a half-time of 0.5 hour; but the transport of hexoses returns to control levels much more slowly, with a half-time of approximately 6 hours. The two processes appear to be independently regulated.", "PMID": 956278}
{"id": "pubmed23n0032_2112", "title": "Effect of ESF preparations on different types of erythroblasts.", "content": "A new method of quantitative (14)C-autoradiography was applied for evaluating possible effects of erythropoietin(ESF) on the DNA synthesis rate of differentiated erythroid murine bone marrow cells identified as proerythroblasts, basophilic and polychromatic erythroblasts...", "contents": "Effect of ESF preparations on different types of erythroblasts. A new method of quantitative (14)C-autoradiography was applied for evaluating possible effects of erythropoietin(ESF) on the DNA synthesis rate of differentiated erythroid murine bone marrow cells identified as proerythroblasts, basophilic and polychromatic erythroblasts...", "PMID": 956279}
{"id": "pubmed23n0032_2113", "title": "Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA.", "content": "Cytoplasmic poly-A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5-bromodeoxyuridine. The kinetics of 3H-adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly-A segment and non-poly-A portion of poly-A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells.", "contents": "Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA. Cytoplasmic poly-A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5-bromodeoxyuridine. The kinetics of 3H-adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly-A segment and non-poly-A portion of poly-A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells.", "PMID": 956280}
{"id": "pubmed23n0032_2114", "title": "Growth abnormalities of cultured human skin fibroblasts derived from individuals with hereditary adenomatosis of the colon and rectum.", "content": "A heritable propensity to develop malignant lesions is found in individuals with familial adenomatosis of the colon an rectum (ACR) and the Gardner's syndrome variant, an autosomal dominant trait. In the present study, the growth characteristics of cultured skin fibroblasts (SF) derived from normal-appearing flat skin biopsies of ACR families, representing all phenotypes, and appropriate controls were investigated. SF were obtained from stocks between the second and fifth passages and growth to confluency in Eagle's Minimal Essential Medium (EMEM) supplemented with 15% fetal calf serum (FCS). Following trypsinization, cells were replanted in EMEM supplemented with either 1% or 15% FCS at an initial density of 4 x 10(3) cells/cm2 and counted daily for five days. Normal SF representing several age groups (both sexes) and those obtained from non-afflicted individuals of ACR families grew only in 15% FCS. In contrast, SF from ACR subjects and from embryonal skin grew both in 1% and 15% FCS. SF from several clinically asymptomatic adults, children or ACR patients, grew in 1% FCS as well. Cell cultures from ACR individuals showed regions of criss-crossed arrays and multilayered pattern. These growth properties were not observed in normal cell cultures. The SF from ACR individuals did not grow in methocel, nor did they form tumors in athymic mice. These results suggest the occurrence of previously undetected biochemical alterations in SF taken from ACR genotypes.", "contents": "Growth abnormalities of cultured human skin fibroblasts derived from individuals with hereditary adenomatosis of the colon and rectum. A heritable propensity to develop malignant lesions is found in individuals with familial adenomatosis of the colon an rectum (ACR) and the Gardner's syndrome variant, an autosomal dominant trait. In the present study, the growth characteristics of cultured skin fibroblasts (SF) derived from normal-appearing flat skin biopsies of ACR families, representing all phenotypes, and appropriate controls were investigated. SF were obtained from stocks between the second and fifth passages and growth to confluency in Eagle's Minimal Essential Medium (EMEM) supplemented with 15% fetal calf serum (FCS). Following trypsinization, cells were replanted in EMEM supplemented with either 1% or 15% FCS at an initial density of 4 x 10(3) cells/cm2 and counted daily for five days. Normal SF representing several age groups (both sexes) and those obtained from non-afflicted individuals of ACR families grew only in 15% FCS. In contrast, SF from ACR subjects and from embryonal skin grew both in 1% and 15% FCS. SF from several clinically asymptomatic adults, children or ACR patients, grew in 1% FCS as well. Cell cultures from ACR individuals showed regions of criss-crossed arrays and multilayered pattern. These growth properties were not observed in normal cell cultures. The SF from ACR individuals did not grow in methocel, nor did they form tumors in athymic mice. These results suggest the occurrence of previously undetected biochemical alterations in SF taken from ACR genotypes.", "PMID": 956281}
{"id": "pubmed23n0032_2115", "title": "Ornithine decarboxylase inactivation in HeLa cells.", "content": "Ornithine decarboxylase (ODC) can be induced up to 100-fold over basal levels four hours after addition of glutamine to the medium of HeLa cells growing in suspension culture. As demonstrated in several other cell types, ODC is inactivated very rapidly in HeLa cells, and the rate of inactivation is seen to vary with a half life of 9-15 minutes in uninduced cells and rises to ca. 60 minutes at the peak of induction. Quantitatively, the change in rate of inactivation cannot completely account for the observed rise in activity, thus synthesis or activation of ODC must also be involved in the induction process. The inactivation process requires metabolic energy and it can be sustained by glycolytic derived energy. Other factors which are known to inhibit protein breakdown in mammalian cells, such as sodium fluoride, insulin, or tosyl phenylalanyl chloromethyl ketone, had no effect on the rate of inactivation of ODC. Attempts to demonstrate ODC inactivation in a cell free system at neutral pH were unsuccessful.", "contents": "Ornithine decarboxylase inactivation in HeLa cells. Ornithine decarboxylase (ODC) can be induced up to 100-fold over basal levels four hours after addition of glutamine to the medium of HeLa cells growing in suspension culture. As demonstrated in several other cell types, ODC is inactivated very rapidly in HeLa cells, and the rate of inactivation is seen to vary with a half life of 9-15 minutes in uninduced cells and rises to ca. 60 minutes at the peak of induction. Quantitatively, the change in rate of inactivation cannot completely account for the observed rise in activity, thus synthesis or activation of ODC must also be involved in the induction process. The inactivation process requires metabolic energy and it can be sustained by glycolytic derived energy. Other factors which are known to inhibit protein breakdown in mammalian cells, such as sodium fluoride, insulin, or tosyl phenylalanyl chloromethyl ketone, had no effect on the rate of inactivation of ODC. Attempts to demonstrate ODC inactivation in a cell free system at neutral pH were unsuccessful.", "PMID": 956282}
{"id": "pubmed23n0032_2116", "title": "Selective isolation of reversible cold sensitive variants from Chinese hamster ovary cell cultures.", "content": "Variants of the Chinese hamster ovary cell line CHO-KI (ATTCCCL61)which grow almost normally at 38.5C but very poorly or not at all at 30C were obtained after treatment with mutagens and application of an indirect selection procedure.  Two kinds of variants were recovered.  In the first of these, the cold sensitive phenotypw is expressed completely only at low cell densities.  At higher cell desity, growth continues at the nonpermissive temperature, but at a reduced rate.  In the second class, the cold snesitive phenotype is independent of cell density.  Two members of the  latter class were studied in detail.  In both lines, after shift to the nonpermissive temperature, the rateof H-thymidine incorporation declines markedly; the rates of H-uridine and H-phenylalanine uptake are less drastically reduced.  Autoradiographs indicate that the decline in thymidine uptake at the nonpermissive temperature is due to an elongation of part of the cell cycle, so that a smaller proportion of the cells lie in the synthetic (S) phase of the cell cycle with a consequent reduction in the fraction labeled cells.  The uridine labeling patterns of the mutants appear to rule out a ribsomal lesion.  Low temperature growth inhibition of both cell strains was reversible.  In one of the cell lines, an apparent stretching of the cells at the low temperature produces substantial alterations in cell shape.", "contents": "Selective isolation of reversible cold sensitive variants from Chinese hamster ovary cell cultures. Variants of the Chinese hamster ovary cell line CHO-KI (ATTCCCL61)which grow almost normally at 38.5C but very poorly or not at all at 30C were obtained after treatment with mutagens and application of an indirect selection procedure.  Two kinds of variants were recovered.  In the first of these, the cold sensitive phenotypw is expressed completely only at low cell densities.  At higher cell desity, growth continues at the nonpermissive temperature, but at a reduced rate.  In the second class, the cold snesitive phenotype is independent of cell density.  Two members of the  latter class were studied in detail.  In both lines, after shift to the nonpermissive temperature, the rateof H-thymidine incorporation declines markedly; the rates of H-uridine and H-phenylalanine uptake are less drastically reduced.  Autoradiographs indicate that the decline in thymidine uptake at the nonpermissive temperature is due to an elongation of part of the cell cycle, so that a smaller proportion of the cells lie in the synthetic (S) phase of the cell cycle with a consequent reduction in the fraction labeled cells.  The uridine labeling patterns of the mutants appear to rule out a ribsomal lesion.  Low temperature growth inhibition of both cell strains was reversible.  In one of the cell lines, an apparent stretching of the cells at the low temperature produces substantial alterations in cell shape.", "PMID": 956283}
{"id": "pubmed23n0032_2117", "title": "Growth of in vitro colony-forming cells from normal human peripheral blood leukocytes cultured in diffusion chambers.", "content": "The growth of granulopoietic progenitor cells (CFU-C) in diffusion chambers during culture of peripheral blood leukocytes from 10 normal subjects has been studied. At various times after initiation of diffusion chamber culture, cells harvested from the chambers were transferred to agar culture for measurement of CFU-C concentration. Under these conditions colonies could be grown successfully in agar culture provided pronase, necessary for the chamber harvesting procedure, was first removed by careful washing. A marked increase in the number of CFU-C, up to 25-fold the initial value, was observed in 8 out of 10 subjects. Here the growth pattern was similar, independent of the initial CFU-C throughout the diffusion chamber culture period was very poor. The growth of CFU-C from a given individual's blood was shown to be reproducible in repeated studies in 2 subjects, one of whom showed a proliferative and the other a non-proliferative pattern. Evidence suggests that the increase in CFU-C in diffusion chambers is the result of both self-renewal of these cells and influx from a more primitive compartment, although the present data do not allow an estimate of the relative magnitude of each.", "contents": "Growth of in vitro colony-forming cells from normal human peripheral blood leukocytes cultured in diffusion chambers. The growth of granulopoietic progenitor cells (CFU-C) in diffusion chambers during culture of peripheral blood leukocytes from 10 normal subjects has been studied. At various times after initiation of diffusion chamber culture, cells harvested from the chambers were transferred to agar culture for measurement of CFU-C concentration. Under these conditions colonies could be grown successfully in agar culture provided pronase, necessary for the chamber harvesting procedure, was first removed by careful washing. A marked increase in the number of CFU-C, up to 25-fold the initial value, was observed in 8 out of 10 subjects. Here the growth pattern was similar, independent of the initial CFU-C throughout the diffusion chamber culture period was very poor. The growth of CFU-C from a given individual's blood was shown to be reproducible in repeated studies in 2 subjects, one of whom showed a proliferative and the other a non-proliferative pattern. Evidence suggests that the increase in CFU-C in diffusion chambers is the result of both self-renewal of these cells and influx from a more primitive compartment, although the present data do not allow an estimate of the relative magnitude of each.", "PMID": 956284}
{"id": "pubmed23n0032_2118", "title": "[Wirsung's duct and chronic pancreatitis. Changes in the morphology of the pancreatic ducts after pancreatic duct shunts. 23 cases followed-up].", "content": "The authors study in chronic pancreatitis the morphology of Wirsung's duct in 31 patients who had undergone repeated operations, 23 of them were submitted in a first stage to an anastomosis between the pancreatic duct and the digestive tract. The main causes of failure were obstructions of the anastomosis, biliary complications and continuation of the pancreatic disease. The difference in prognosis between pancreatitis with a dilated pancreatic duct, and those with a filiform duct, is perhaps due to lesions of different histological appearance and course. The best results were obtained in patients able to give up alcohol and in whom it was possible to carry out a broader anastomosis on a dilated and unobstructed pancreatic duct.", "contents": "[Wirsung's duct and chronic pancreatitis. Changes in the morphology of the pancreatic ducts after pancreatic duct shunts. 23 cases followed-up]. The authors study in chronic pancreatitis the morphology of Wirsung's duct in 31 patients who had undergone repeated operations, 23 of them were submitted in a first stage to an anastomosis between the pancreatic duct and the digestive tract. The main causes of failure were obstructions of the anastomosis, biliary complications and continuation of the pancreatic disease. The difference in prognosis between pancreatitis with a dilated pancreatic duct, and those with a filiform duct, is perhaps due to lesions of different histological appearance and course. The best results were obtained in patients able to give up alcohol and in whom it was possible to carry out a broader anastomosis on a dilated and unobstructed pancreatic duct.", "PMID": 956285}
{"id": "pubmed23n0032_2119", "title": "[Surgical treatment of stage II arteriopathies of the lower limbs].", "content": "The authors report their results of 218, operations carried out in patients at stage II of peripheral arterial disease during the period 1970 to 1974. In 138 patients who were submitted to direct surgery, the results appeared specially satisfactory in cases of aorto-iliac and ilio-femoral obstruction (83 and 87 p. 100 good results at 4 years). They were still 76 p. 100 in cases of femoro-popliteal obstruction.", "contents": "[Surgical treatment of stage II arteriopathies of the lower limbs]. The authors report their results of 218, operations carried out in patients at stage II of peripheral arterial disease during the period 1970 to 1974. In 138 patients who were submitted to direct surgery, the results appeared specially satisfactory in cases of aorto-iliac and ilio-femoral obstruction (83 and 87 p. 100 good results at 4 years). They were still 76 p. 100 in cases of femoro-popliteal obstruction.", "PMID": 956286}
{"id": "pubmed23n0032_2120", "title": "[Contribution of scintigraphy in acute and chronic pancreatitis. Apropos of 200 examinations].", "content": "The authors report 200 examinations and discuss the role of radio-isotope scans of the pancreas with electronic subtraction of the liver image in acute and chronic pancreatitis. As an emergency, the examination is of definite diagnostic value, the absence of fixation with a suggestive clinical context, is strong evidence of pancreatic disease and normal uptake eliminates the latter. On the other hand, in painful syndromes in which chronic pancreatitis is suggested, the diagnostic interest is very limited for the low uptake on radio-isotope scan occurs at a late stage and is not specific. Radio-isotope scans are worth a place in exploration of the pancreas in acute pancreatitis.", "contents": "[Contribution of scintigraphy in acute and chronic pancreatitis. Apropos of 200 examinations]. The authors report 200 examinations and discuss the role of radio-isotope scans of the pancreas with electronic subtraction of the liver image in acute and chronic pancreatitis. As an emergency, the examination is of definite diagnostic value, the absence of fixation with a suggestive clinical context, is strong evidence of pancreatic disease and normal uptake eliminates the latter. On the other hand, in painful syndromes in which chronic pancreatitis is suggested, the diagnostic interest is very limited for the low uptake on radio-isotope scan occurs at a late stage and is not specific. Radio-isotope scans are worth a place in exploration of the pancreas in acute pancreatitis.", "PMID": 956287}
{"id": "pubmed23n0032_2121", "title": "[Revascularization of arteries of the leg by bypass in chronic arteriopathies of the lower limbs. Apropos of 50 cases].", "content": "The authors report 50 by-pass operations followed up for from 6 months to 4 years, which were designed to restore the blood supply via the leg arteries in peripheral arterial disease of the lower limbs. 40 patients were at stages III and IV; acute ischemia required emergency operation in 6 cases. 2 Patients died immediately after operation. 30 by-passes remained permeable. A cure of the trophic disorder and a functional improvement were obtained in 39 patients with only 8 subsequent amputations. In the light of the world literature and their personal experience they discuss the main pronostic factors and the indications for this method which has proved to be a remarkable progress in the treatment of severe arterial disease of the lower limbs.", "contents": "[Revascularization of arteries of the leg by bypass in chronic arteriopathies of the lower limbs. Apropos of 50 cases]. The authors report 50 by-pass operations followed up for from 6 months to 4 years, which were designed to restore the blood supply via the leg arteries in peripheral arterial disease of the lower limbs. 40 patients were at stages III and IV; acute ischemia required emergency operation in 6 cases. 2 Patients died immediately after operation. 30 by-passes remained permeable. A cure of the trophic disorder and a functional improvement were obtained in 39 patients with only 8 subsequent amputations. In the light of the world literature and their personal experience they discuss the main pronostic factors and the indications for this method which has proved to be a remarkable progress in the treatment of severe arterial disease of the lower limbs.", "PMID": 956288}
{"id": "pubmed23n0032_2122", "title": "[Amputations for arteritis. Criteria of choice of the level of the amputation].", "content": "The practice in arteritis, of economic amputations conserving the knee, is justified by the better quality of the functional results obtained and, also, the risk of later bilateral amputation. This risk, in the series studied here was estimated at 21.6 p. 100. This conservative attitude does, however, have certain risks, the main risk being the necessity for a new amputation at a higher level. The proportion of failures leading to high mid-thigh amputation was of the order of 22 p. 100. To ensure the best possible security in economic amputations, it is important to respect a certain number of criteria which are studied here: the general and psychological condition of the patient, the arteriographic findings, the functional investigations and, finally, the operative test which assesses the vitality of the tissues at the level of the area of section.", "contents": "[Amputations for arteritis. Criteria of choice of the level of the amputation]. The practice in arteritis, of economic amputations conserving the knee, is justified by the better quality of the functional results obtained and, also, the risk of later bilateral amputation. This risk, in the series studied here was estimated at 21.6 p. 100. This conservative attitude does, however, have certain risks, the main risk being the necessity for a new amputation at a higher level. The proportion of failures leading to high mid-thigh amputation was of the order of 22 p. 100. To ensure the best possible security in economic amputations, it is important to respect a certain number of criteria which are studied here: the general and psychological condition of the patient, the arteriographic findings, the functional investigations and, finally, the operative test which assesses the vitality of the tissues at the level of the area of section.", "PMID": 956289}
{"id": "pubmed23n0032_2123", "title": "[Etiopathogenesis of varicocele and therapeutic conclusions].", "content": "The many pathogenic theories for varicocele, mostly hypothetic, have shown the uncertainty of the cause of the disease including the use of the word \"essential\". Anatomical, phlebomanometric and phlebographic studies determined the main cause of varicocele, the short course of the aorto-mesenteric artery which, by stricture of the left renal vein in the standing position, produces reno-spermatic venous reflux producing varicose dilatation of the left spermatic plexus and, thus, varicocele. In addition to this main cause, the vascular background with a qualitative deficiency of the connective tissue and prolonged standing during work, are the main factors which favour the onset of varicocele. An active, early therapeutic attitude and high ligature of the left spermatic vein give the best results.", "contents": "[Etiopathogenesis of varicocele and therapeutic conclusions]. The many pathogenic theories for varicocele, mostly hypothetic, have shown the uncertainty of the cause of the disease including the use of the word \"essential\". Anatomical, phlebomanometric and phlebographic studies determined the main cause of varicocele, the short course of the aorto-mesenteric artery which, by stricture of the left renal vein in the standing position, produces reno-spermatic venous reflux producing varicose dilatation of the left spermatic plexus and, thus, varicocele. In addition to this main cause, the vascular background with a qualitative deficiency of the connective tissue and prolonged standing during work, are the main factors which favour the onset of varicocele. An active, early therapeutic attitude and high ligature of the left spermatic vein give the best results.", "PMID": 956290}
{"id": "pubmed23n0032_2124", "title": "[Jejuno and ileo-ileal invaginations revealing mutiple melanic metastases of the small intestine. Case report].", "content": "The authors report the case of 60 year old man with multiple jeuno-ileal metastases from a malignant melanoma of the skin revealed by jejuno-jejunal intussusceptions. In the light of this case, the authors emphasize the frequency and preferential localisation of these metastases at the level of the jejunum and ileum, the usual mildness of their symptoms until the day where acute obstruction requires laparotomy. The multiple lesions, their diversity and their very rapid course.", "contents": "[Jejuno and ileo-ileal invaginations revealing mutiple melanic metastases of the small intestine. Case report]. The authors report the case of 60 year old man with multiple jeuno-ileal metastases from a malignant melanoma of the skin revealed by jejuno-jejunal intussusceptions. In the light of this case, the authors emphasize the frequency and preferential localisation of these metastases at the level of the jejunum and ileum, the usual mildness of their symptoms until the day where acute obstruction requires laparotomy. The multiple lesions, their diversity and their very rapid course.", "PMID": 956291}
{"id": "pubmed23n0032_2125", "title": "[Treatment of cutaneous nevocarcinomas].", "content": "For long, malignant melanoma of the skin was never treated other than surgically; in spite of considerable effort in this field, a large number of them remained with a very poor prognosis. In the severest cases, it was noted that the prognosis was little influenced by recent therapeutic progress. The latter, however, in the light of recent experience, seemed to justify the hope which was placed in it initially. The object of this report is to relate modern progress in the treatment based on clinical and pathological findings. It is the result of work undertaken during recent years by the research group on malignant melanomas of the Lariboisi\u00e9re-Saint-Louis hospitals.", "contents": "[Treatment of cutaneous nevocarcinomas]. For long, malignant melanoma of the skin was never treated other than surgically; in spite of considerable effort in this field, a large number of them remained with a very poor prognosis. In the severest cases, it was noted that the prognosis was little influenced by recent therapeutic progress. The latter, however, in the light of recent experience, seemed to justify the hope which was placed in it initially. The object of this report is to relate modern progress in the treatment based on clinical and pathological findings. It is the result of work undertaken during recent years by the research group on malignant melanomas of the Lariboisi\u00e9re-Saint-Louis hospitals.", "PMID": 956292}
{"id": "pubmed23n0032_2126", "title": "[Research on intrazygoportal disconnection by use of Vosschulte's ring. Experimental study in the dog].", "content": "The disappointing results of porta-caval anastomosis in the treatment of portal hypertension have led surgeons to treat directly oesphageal varices by interazygoportal disconnection through Vosschulte's ring. An experimental study on 29 dogs, permitted the authors to make several discoveries. The ring introduced through the pylorotomy should have a caliber identical to that of the oesphagus. The course of the stricture then occurs towards mutilant annular fibrosis of all the oesphageal wall. The marked morbidity due to regurgitation linked to stenosis, may be reduced by early instrumental dilatation.", "contents": "[Research on intrazygoportal disconnection by use of Vosschulte's ring. Experimental study in the dog]. The disappointing results of porta-caval anastomosis in the treatment of portal hypertension have led surgeons to treat directly oesphageal varices by interazygoportal disconnection through Vosschulte's ring. An experimental study on 29 dogs, permitted the authors to make several discoveries. The ring introduced through the pylorotomy should have a caliber identical to that of the oesphagus. The course of the stricture then occurs towards mutilant annular fibrosis of all the oesphageal wall. The marked morbidity due to regurgitation linked to stenosis, may be reduced by early instrumental dilatation.", "PMID": 956293}
{"id": "pubmed23n0032_2127", "title": "[Pre-operative irradiation in the treatment of cancers of thoracic esophagus].", "content": "The authors analyse an homogeneous series (2 operators) of 650 patients operated on for thoracic oesophageal carcinoma, of which 185 were submitted to pre-operative irradiation with cobalt. Irradiation led to a definite increase in the resectability rate 74 p. 100 as against 62 p. 100. There was an increase in the mid-term survival rate, especially an increase of 5 p. 100 at 2 years, 8 p. 100 at 3 years, 6 p. 100 at 4 years; The 5 survival rate was little modified. It was 12 p. 100 for 290 cases, resection alone, and 15 p. 100 for 137 resections after irradiation.", "contents": "[Pre-operative irradiation in the treatment of cancers of thoracic esophagus]. The authors analyse an homogeneous series (2 operators) of 650 patients operated on for thoracic oesophageal carcinoma, of which 185 were submitted to pre-operative irradiation with cobalt. Irradiation led to a definite increase in the resectability rate 74 p. 100 as against 62 p. 100. There was an increase in the mid-term survival rate, especially an increase of 5 p. 100 at 2 years, 8 p. 100 at 3 years, 6 p. 100 at 4 years; The 5 survival rate was little modified. It was 12 p. 100 for 290 cases, resection alone, and 15 p. 100 for 137 resections after irradiation.", "PMID": 956294}
{"id": "pubmed23n0032_2128", "title": "[Surgical treatment of villous tumors of the rectum].", "content": "Out of 36 villous tumours observed in 31 patients, 50 p. 100 were sessile and 1/3rd were degenerated, their degeneration was much more frequent in the case of sessile villous tumours. There were 5 biopsy errors. Out of 23 villous tumours, class A, 11 underwent local removal with two relapses, 7 recto-sigmoidal resections and 3 amputations of the rectum and 2 Hartmann operations. Out of 12 villous tumours classified as B and C, 5 underwent local removal, 4 resections, 3 palliative amputations. In all, there were 5 relapses. Tumours class C are severe and have the same prognosis as carcinoma of the rectum. Certain benign villous tumours are very difficult to remove locally owing to their broad base and their volume, which may necessitate mutilation. The present therapeutic attitude of the authors is due to the severity of relapses which may be seen late and possible biopsy errors. Broad removal is recommended for benign villous tumours which are broadly implanted and very voluminous. If the villous tumour is above the pouch of Douglas, resection is preferable to local removal. On the other hand, local removal is justified for benign villous tumours placed above the pouch of Douglas, which may be pediculated or sessile with a narrow base and with normal neighbouring mucosa.", "contents": "[Surgical treatment of villous tumors of the rectum]. Out of 36 villous tumours observed in 31 patients, 50 p. 100 were sessile and 1/3rd were degenerated, their degeneration was much more frequent in the case of sessile villous tumours. There were 5 biopsy errors. Out of 23 villous tumours, class A, 11 underwent local removal with two relapses, 7 recto-sigmoidal resections and 3 amputations of the rectum and 2 Hartmann operations. Out of 12 villous tumours classified as B and C, 5 underwent local removal, 4 resections, 3 palliative amputations. In all, there were 5 relapses. Tumours class C are severe and have the same prognosis as carcinoma of the rectum. Certain benign villous tumours are very difficult to remove locally owing to their broad base and their volume, which may necessitate mutilation. The present therapeutic attitude of the authors is due to the severity of relapses which may be seen late and possible biopsy errors. Broad removal is recommended for benign villous tumours which are broadly implanted and very voluminous. If the villous tumour is above the pouch of Douglas, resection is preferable to local removal. On the other hand, local removal is justified for benign villous tumours placed above the pouch of Douglas, which may be pediculated or sessile with a narrow base and with normal neighbouring mucosa.", "PMID": 956295}
{"id": "pubmed23n0032_2129", "title": "[Uretero-ileoplasty in bilharzian uretero-hydronephrosis].", "content": "The authors report 22 cases of uretero-ileo-plasty for uretero-hydronephrosis due to schistosomiasis. The indications for operation depend on the following criteria: the degree of dilatation which varies from simple atonia to very large hydronephrosis which one must not wait for, ureteral stenosis, vesico-ureteral reflux, the degree of renal failure assessed by studies of creatinine and urea clearance and the resistance to treatment. The operative technique which is not specific for bilharziasis includes uretero-ileo-plasty which is often bilateral, for even in asymmetrical cases, the least affected ureter is often of poor quality. There were failures in two cases due to irreversible renal failure, and in two cases, due to peritonitis. The late results of the other cases appear very favourable: increased vesical capacity, diminution of cystalgia, comfort and improved, general health, were the main factors. Stenosis of the anastomosis, vesico-ureteral reflux and urinary infection, acidosis, lithiasis are rare or not very severe.", "contents": "[Uretero-ileoplasty in bilharzian uretero-hydronephrosis]. The authors report 22 cases of uretero-ileo-plasty for uretero-hydronephrosis due to schistosomiasis. The indications for operation depend on the following criteria: the degree of dilatation which varies from simple atonia to very large hydronephrosis which one must not wait for, ureteral stenosis, vesico-ureteral reflux, the degree of renal failure assessed by studies of creatinine and urea clearance and the resistance to treatment. The operative technique which is not specific for bilharziasis includes uretero-ileo-plasty which is often bilateral, for even in asymmetrical cases, the least affected ureter is often of poor quality. There were failures in two cases due to irreversible renal failure, and in two cases, due to peritonitis. The late results of the other cases appear very favourable: increased vesical capacity, diminution of cystalgia, comfort and improved, general health, were the main factors. Stenosis of the anastomosis, vesico-ureteral reflux and urinary infection, acidosis, lithiasis are rare or not very severe.", "PMID": 956296}
{"id": "pubmed23n0032_2130", "title": "[Diagnostic traps and procedure to follow in radio-invisible biliary calculi].", "content": "Instead of the term biliary microlithiasis, the authors prefer the general concept of radio-invisible biliary lithiasis. The size, the homogeneity, the site and chemical composition of the gall stones, which are the usual criteria of biliary microlithiasis, also are valid for radio-invisibility; thus the diagnostic traps are the only original characteristics of a pathology with hazy outlines, defined as one which escapes well conducted a radiological exploration. Biliary micro-lithiasis therefore raises a triple problem. The indications for biliary surgical exploration, in spite of normal cholangiography, seem to us undoubted in acute relapsing pancreatitis, once the usual medical causes have been eliminated. It should be more relative in pure biliary pain which first requires, not only repeated cholangiography, but also constant clinical and laboratory signs which suggest that the symptoms are organic and that gall stones are in formation. After laparotomy, cholecystectomy is required, not only in perceptible lithiasis, but also when the gall bladder bile contains mud, d\u00e9bris, sand or cholesterol spheroids. In the other cases, the choice between abstention or cholecystectomy depends on the clinical and laboratory context. The surgical attitude with regard to the common bile duct is discussed in three possibilities depending on the case, e.g. routine exploration, abstention or exploration in certain cases.", "contents": "[Diagnostic traps and procedure to follow in radio-invisible biliary calculi]. Instead of the term biliary microlithiasis, the authors prefer the general concept of radio-invisible biliary lithiasis. The size, the homogeneity, the site and chemical composition of the gall stones, which are the usual criteria of biliary microlithiasis, also are valid for radio-invisibility; thus the diagnostic traps are the only original characteristics of a pathology with hazy outlines, defined as one which escapes well conducted a radiological exploration. Biliary micro-lithiasis therefore raises a triple problem. The indications for biliary surgical exploration, in spite of normal cholangiography, seem to us undoubted in acute relapsing pancreatitis, once the usual medical causes have been eliminated. It should be more relative in pure biliary pain which first requires, not only repeated cholangiography, but also constant clinical and laboratory signs which suggest that the symptoms are organic and that gall stones are in formation. After laparotomy, cholecystectomy is required, not only in perceptible lithiasis, but also when the gall bladder bile contains mud, d\u00e9bris, sand or cholesterol spheroids. In the other cases, the choice between abstention or cholecystectomy depends on the clinical and laboratory context. The surgical attitude with regard to the common bile duct is discussed in three possibilities depending on the case, e.g. routine exploration, abstention or exploration in certain cases.", "PMID": 956297}
{"id": "pubmed23n0032_2131", "title": "[Synovectomies of the knee. Apropos of 47 cases tuberculous synovitis].", "content": "The authors carried out synovectomy of the knee in 70 cases, including 47 for tuberculous synovitis, with follow up periods of 2 years and half to 18 years. The subjects included: -30 children from 2 to 15 years; -17 adults from 16 to 50 years, with 3 cases of more than 50 years. Very good: greater or equal to 110 degrees in 2 cases; -Good: greater or equal to 90 degrees in 26 cases; -Average: greater or equal to 45 to 90 degrees in 11 cases; -Poor: less than 45 degrees in 8 cases. The authors believe that synovectomy in tuberculous synovitis of the knee without an osteocartilagenous lesion, or with slight lesions of the joint is more efficacious than medical treatment alone and may shorten the duration of treatment.", "contents": "[Synovectomies of the knee. Apropos of 47 cases tuberculous synovitis]. The authors carried out synovectomy of the knee in 70 cases, including 47 for tuberculous synovitis, with follow up periods of 2 years and half to 18 years. The subjects included: -30 children from 2 to 15 years; -17 adults from 16 to 50 years, with 3 cases of more than 50 years. Very good: greater or equal to 110 degrees in 2 cases; -Good: greater or equal to 90 degrees in 26 cases; -Average: greater or equal to 45 to 90 degrees in 11 cases; -Poor: less than 45 degrees in 8 cases. The authors believe that synovectomy in tuberculous synovitis of the knee without an osteocartilagenous lesion, or with slight lesions of the joint is more efficacious than medical treatment alone and may shorten the duration of treatment.", "PMID": 956298}
{"id": "pubmed23n0032_2132", "title": "[Stenosis of the small intestine after strangulated hernia].", "content": "Return to the abdominal cavity of an intestinal loop which had suffered from ischemia, may induce, after a free interval, disordered transit or frank intestinal obstruction, sometimes peritonitis. This is always dangerous, especially in elderly patients. The authors report 5 cases which illustrate this danger, in daily surgery, and recall the rules for treatment of strangulated hernia.", "contents": "[Stenosis of the small intestine after strangulated hernia]. Return to the abdominal cavity of an intestinal loop which had suffered from ischemia, may induce, after a free interval, disordered transit or frank intestinal obstruction, sometimes peritonitis. This is always dangerous, especially in elderly patients. The authors report 5 cases which illustrate this danger, in daily surgery, and recall the rules for treatment of strangulated hernia.", "PMID": 956299}
{"id": "pubmed23n0032_2133", "title": "[5 kg sternal chondroma, treated by resection and silastic prosthesis. Satisfactory result].", "content": "Resection of the sternum and adjacent, cartilages raises difficult problems of reconstruction. The tumour, which, exceeding 5 kg was considered histologically as a chondroma, was replaced by a silastic prosthesis with an excellent result 13 months later.", "contents": "[5 kg sternal chondroma, treated by resection and silastic prosthesis. Satisfactory result]. Resection of the sternum and adjacent, cartilages raises difficult problems of reconstruction. The tumour, which, exceeding 5 kg was considered histologically as a chondroma, was replaced by a silastic prosthesis with an excellent result 13 months later.", "PMID": 956300}
{"id": "pubmed23n0032_2134", "title": "[Route of approach of distal deep femoral artery].", "content": "Proximal implantation of by-passes between the femoral and popliteal or femoral and leg arteries, may be carried out on the middle portion of the profunda femoris artery, between the first and second perforating arteries. An approach some distance from Scarpa's triangle, permits one to reach fairly easily, within a muscular triangle between the vastus medialis and the adductor longus. Two cases illustrate the interest of this route of approach described in Lyons for the first time in 1970.", "contents": "[Route of approach of distal deep femoral artery]. Proximal implantation of by-passes between the femoral and popliteal or femoral and leg arteries, may be carried out on the middle portion of the profunda femoris artery, between the first and second perforating arteries. An approach some distance from Scarpa's triangle, permits one to reach fairly easily, within a muscular triangle between the vastus medialis and the adductor longus. Two cases illustrate the interest of this route of approach described in Lyons for the first time in 1970.", "PMID": 956303}
{"id": "pubmed23n0032_2135", "title": "[Our indications in surgical treatment of supra-condylar fractures of the femur].", "content": "The treatment of supracondylar fractures of the femur should be surgical. The authors consider the requirements of a strict technic of osteosynthesis. The use for this purpose: either a plate and strip, or a plate and screw, and justify their respective indications.", "contents": "[Our indications in surgical treatment of supra-condylar fractures of the femur]. The treatment of supracondylar fractures of the femur should be surgical. The authors consider the requirements of a strict technic of osteosynthesis. The use for this purpose: either a plate and strip, or a plate and screw, and justify their respective indications.", "PMID": 956304}
{"id": "pubmed23n0032_2136", "title": "Gas chromatographic analysis of heptafluorobutyryl derivatives of some carbamate insecticides.", "content": "The gas chromatography (GC) of a number of carbamate insecticides as their heptafluorobutyryl derivatives has been studied. The reaction makes use of heptafluorobutyric anhydride with trimethylamine in benzene as a catalyst. Conversion of the insecticides to their products is complete in 15-20 min at room temperature. The same reaction proved satisfactory for trifluoroacetylation. The excess reagent and trimethylamine are removed by partitioning with water in the reaction test tube. An aliquot of the organic phase is used for analysis. The heptafluorobutyryl derivatives are stable for several days on the lab bench without removal of the aqueous phase from the test tube. GC detection was carried out with a Coulson conductivity detector in the halogen (reductive) mode. About 1.0 ng produced a 50% full-scale response for most of the carbamates (3 min retention time) on 3% OV-1. This method was applied to the analysis of several carbamates spiked in corn, mustard greens, turnip greens, lettuce and cabbage.", "contents": "Gas chromatographic analysis of heptafluorobutyryl derivatives of some carbamate insecticides. The gas chromatography (GC) of a number of carbamate insecticides as their heptafluorobutyryl derivatives has been studied. The reaction makes use of heptafluorobutyric anhydride with trimethylamine in benzene as a catalyst. Conversion of the insecticides to their products is complete in 15-20 min at room temperature. The same reaction proved satisfactory for trifluoroacetylation. The excess reagent and trimethylamine are removed by partitioning with water in the reaction test tube. An aliquot of the organic phase is used for analysis. The heptafluorobutyryl derivatives are stable for several days on the lab bench without removal of the aqueous phase from the test tube. GC detection was carried out with a Coulson conductivity detector in the halogen (reductive) mode. About 1.0 ng produced a 50% full-scale response for most of the carbamates (3 min retention time) on 3% OV-1. This method was applied to the analysis of several carbamates spiked in corn, mustard greens, turnip greens, lettuce and cabbage.", "PMID": 956309}
{"id": "pubmed23n0032_2137", "title": "Sephadex LH-20 multiple-column chromatography for the simultaneous separation of progesterone, deoxycorticosterone and 17alpha-hydroxyprogesterone from small plasma samples.", "content": "A simple and convenient chromatographic method is described for the simultaneous and complete separation of the unconjugated steroids progesterone, deoxycorticosterone and 17 alpha-hydroxyprogesterone on 40-cm columns of Sephadex LH-20 using a water-saturated solvent system containing n-heptane-chloroform (1:1) plus 0.25% of ethanol. Manual operation of up to ten columns run in parallel is facilitated by the use of graduated, cylindrical, solvent reservoirs on top of the columns. Thus, negligible column-to-column, and only limited day-to-day, variations occur in the elution patterns, and constant high recoveries are obtained. When combined with a previously described 60-cm LH-20 chromatography, eight important corticosteroids can be isolated individually from 16 to 20 small plasma samples per day.", "contents": "Sephadex LH-20 multiple-column chromatography for the simultaneous separation of progesterone, deoxycorticosterone and 17alpha-hydroxyprogesterone from small plasma samples. A simple and convenient chromatographic method is described for the simultaneous and complete separation of the unconjugated steroids progesterone, deoxycorticosterone and 17 alpha-hydroxyprogesterone on 40-cm columns of Sephadex LH-20 using a water-saturated solvent system containing n-heptane-chloroform (1:1) plus 0.25% of ethanol. Manual operation of up to ten columns run in parallel is facilitated by the use of graduated, cylindrical, solvent reservoirs on top of the columns. Thus, negligible column-to-column, and only limited day-to-day, variations occur in the elution patterns, and constant high recoveries are obtained. When combined with a previously described 60-cm LH-20 chromatography, eight important corticosteroids can be isolated individually from 16 to 20 small plasma samples per day.", "PMID": 956310}
{"id": "pubmed23n0032_2138", "title": "[Injuries of Lisfranc's joint: severe sprains, dislocations, fractures. Study of 39 personal cases and biomechanical classification].", "content": "A study of 39 cases permitted us an analysis of the lesions of Lisfranc's joint, from severe fracture dislocation to fractures which are rarely reported in this joint. Standard XRay views seem to us insufficient and we propose carrying out oblique views and, sometimes, tomographic views. We were thus able to obtain information permitting a mechanical functional classification, and thus interpret the lesions in relation to the cause and thus adapt treatment. A long-term follow up of 23 cases suggests frequently invaliding sequelae after involvement of the tarso-metatarsal joints. The authors suggest resection and arthrodesis straightaway in dislocations.", "contents": "[Injuries of Lisfranc's joint: severe sprains, dislocations, fractures. Study of 39 personal cases and biomechanical classification]. A study of 39 cases permitted us an analysis of the lesions of Lisfranc's joint, from severe fracture dislocation to fractures which are rarely reported in this joint. Standard XRay views seem to us insufficient and we propose carrying out oblique views and, sometimes, tomographic views. We were thus able to obtain information permitting a mechanical functional classification, and thus interpret the lesions in relation to the cause and thus adapt treatment. A long-term follow up of 23 cases suggests frequently invaliding sequelae after involvement of the tarso-metatarsal joints. The authors suggest resection and arthrodesis straightaway in dislocations.", "PMID": 956305}
{"id": "pubmed23n0032_2139", "title": "Thin-layer chromatography for detection of peptide cleavage or integrity during reactions of the Z-alanylglycines with aniline or phenylhydrazine under papain catalysis.", "content": "Z-l-Ala-gly and Z-dl-ala-gly can yield anilides and phenylhydrazides through peptide cleavage or peptide integrity during papain-catalyzed reactions with aniline (NH2Ph) or phenylhydrazine (NH2NHPh). Since Z-d-ala-gly yielded only uncleaved Z-d-ala-gly-NHPh or Z-d-ala-gly-NHNHPh, these were used as standards in thinlayer chromatography (TLC) for detecting integrity. Known Z-l-ala-NHPh and Z-l-ala-NHNHPh were the standards for cleavage. Depending on the time of incubation, cleaved or unsplit products, or both, were readily detected. The solvent systems that were used for TLC were also effectively employed for separations of reasonable amounts of mixtures of cleaved and uncleaved products through thick-layer chromatography on Chrom AR. This was followed by isolation of the separated components.", "contents": "Thin-layer chromatography for detection of peptide cleavage or integrity during reactions of the Z-alanylglycines with aniline or phenylhydrazine under papain catalysis. Z-l-Ala-gly and Z-dl-ala-gly can yield anilides and phenylhydrazides through peptide cleavage or peptide integrity during papain-catalyzed reactions with aniline (NH2Ph) or phenylhydrazine (NH2NHPh). Since Z-d-ala-gly yielded only uncleaved Z-d-ala-gly-NHPh or Z-d-ala-gly-NHNHPh, these were used as standards in thinlayer chromatography (TLC) for detecting integrity. Known Z-l-ala-NHPh and Z-l-ala-NHNHPh were the standards for cleavage. Depending on the time of incubation, cleaved or unsplit products, or both, were readily detected. The solvent systems that were used for TLC were also effectively employed for separations of reasonable amounts of mixtures of cleaved and uncleaved products through thick-layer chromatography on Chrom AR. This was followed by isolation of the separated components.", "PMID": 956311}
{"id": "pubmed23n0032_2140", "title": "Gas-liquid chromatography of nucleosides. Derivatization and chromatography.", "content": "The aims of this investigation were to establish the optimum reaction conditions for silylation of nucleotides with bis(trimethylsilyl)trifluoroacetamide (BSTFA) and to investigate the chromatographic properties of the following nucleosides: adenosine, guanosine, cytidine, thymine, inosine, xanthosine, and uridine. Closed tube silylations were performed with a 1000 molar excess of BSTFA at 25, 75, 120, 150, and 175 degrees for 15, 30, 60, 120 and 240 min. The optimal time and temperature for derivatization were found to be 150 degrees and 15 min. Using these reaction conditions, samples were then silylated with 50, 100, 200, 500, and 1000 molar excess of BSTFA; a molar excess of 225 was best. The stability of the nucleoside derivatives on standing at room temperature for 1-7 days was investigated. Quantitative gas-liquid chromatography of trimethylsilyl (TMS) nucleosides can be performed if samples are analyzed within 48 h, after which time the relative weight response for the nucleosides decreased somewhat. Chromatographic column studies were made using various liquid phases and supports. With methylsiloxanes as the liquid phase, Supelcoport as support was found to be superior. Resolution of TMS guanosine from TMS cytidine was attempted at different column lengths using 3% (w/w) SE-30 on Supelcoport, different loadings (%, w/w) of SE-30 on Supelcoport, and different polarity liquid phases, 4% (w/w) OV-11 or 3% (w/w) OV-17 or 4% (w/w) Dexsil-300 on Supelcoport. A complete separation of the six ribonucleosides including guanosine and cytidine was obtained with 1 m x 4 mm I.D. glass columns of 4% (w/w) OV-11 and 3% (w/w) OV-17 on 100-120 mesh Supelcoport. It was observed that with 2'-deoxycytidine, one obtains the TMS cytosine peak (retention temperature 150 degrees) plus another peak with a retention temperature of 120 degrees, under all derivatization conditions with BSTFA and bis(trimethylsilyl)-acetamide. Similar formation of bases from other 2'-deoxyribonucleosides occurred when the molar excess of BSTFA was greater than 500. The minimal detectable amounts obtained for all the nucleosides ranged from 5 to 10 ng injected with a signal-to-noise ratio of 3. The relative standard deviations for all nucleosides and deoxynucleosides ranged from 1.2 to 4.8% on different methylsiloxanes on Supelcoport columns.", "contents": "Gas-liquid chromatography of nucleosides. Derivatization and chromatography. The aims of this investigation were to establish the optimum reaction conditions for silylation of nucleotides with bis(trimethylsilyl)trifluoroacetamide (BSTFA) and to investigate the chromatographic properties of the following nucleosides: adenosine, guanosine, cytidine, thymine, inosine, xanthosine, and uridine. Closed tube silylations were performed with a 1000 molar excess of BSTFA at 25, 75, 120, 150, and 175 degrees for 15, 30, 60, 120 and 240 min. The optimal time and temperature for derivatization were found to be 150 degrees and 15 min. Using these reaction conditions, samples were then silylated with 50, 100, 200, 500, and 1000 molar excess of BSTFA; a molar excess of 225 was best. The stability of the nucleoside derivatives on standing at room temperature for 1-7 days was investigated. Quantitative gas-liquid chromatography of trimethylsilyl (TMS) nucleosides can be performed if samples are analyzed within 48 h, after which time the relative weight response for the nucleosides decreased somewhat. Chromatographic column studies were made using various liquid phases and supports. With methylsiloxanes as the liquid phase, Supelcoport as support was found to be superior. Resolution of TMS guanosine from TMS cytidine was attempted at different column lengths using 3% (w/w) SE-30 on Supelcoport, different loadings (%, w/w) of SE-30 on Supelcoport, and different polarity liquid phases, 4% (w/w) OV-11 or 3% (w/w) OV-17 or 4% (w/w) Dexsil-300 on Supelcoport. A complete separation of the six ribonucleosides including guanosine and cytidine was obtained with 1 m x 4 mm I.D. glass columns of 4% (w/w) OV-11 and 3% (w/w) OV-17 on 100-120 mesh Supelcoport. It was observed that with 2'-deoxycytidine, one obtains the TMS cytosine peak (retention temperature 150 degrees) plus another peak with a retention temperature of 120 degrees, under all derivatization conditions with BSTFA and bis(trimethylsilyl)-acetamide. Similar formation of bases from other 2'-deoxyribonucleosides occurred when the molar excess of BSTFA was greater than 500. The minimal detectable amounts obtained for all the nucleosides ranged from 5 to 10 ng injected with a signal-to-noise ratio of 3. The relative standard deviations for all nucleosides and deoxynucleosides ranged from 1.2 to 4.8% on different methylsiloxanes on Supelcoport columns.", "PMID": 956312}
{"id": "pubmed23n0032_2141", "title": "[A new methods of gastroplasty after total gastrectomy].", "content": "The author describes a new procedure which he has studied himself in order to restore alimentary transit after total gastrectomy for carcinoma. This requires raising a jejunal loop using Roux' technique in the retrocolic position and anastomosis to the end of the duodenum below, and end-to-side anastomosis is then made with the end of the esophagus. Finally, the author discusses the advantages of this operation.", "contents": "[A new methods of gastroplasty after total gastrectomy]. The author describes a new procedure which he has studied himself in order to restore alimentary transit after total gastrectomy for carcinoma. This requires raising a jejunal loop using Roux' technique in the retrocolic position and anastomosis to the end of the duodenum below, and end-to-side anastomosis is then made with the end of the esophagus. Finally, the author discusses the advantages of this operation.", "PMID": 956308}
{"id": "pubmed23n0032_2142", "title": "Separation of nucleic acid hydrolysis products, purines, pyrimidines, nucleosides, nucleotides, ribonucleic acid hydrolyzates, and mixtures from nucleotide syntheses by column chromatography on amberlite XAD-4.", "content": "Amberlite XAD-4 resin has been studied as a support for liquid-solid column chromatography. By coating the resin with triethylammonium bicarbonate, a new and unique separation of nucleic acid components has been achieved. Separations are accomplished with a linear gradient of this buffer from 0.1 to 0.4 M. Separation occurs in the following order: inorganic phosphate, purine or pyrimidine bases, 5'-monophosphates, nucleosides and 5'-diphosphates or 5'-triphosphates; the 2'(3')-monophosphates are eluted after either the 5'mono-, di-or triphosphates. The bases and nucleosides are separated in the order: cytosine, uracil, guanine and adenine. Inorganic phosphate and the nucleotides are eluted in the order: inorganic phosphate 5'-mono, di- and tri-phosphates. Excellent separation of the 5'-monophosphates and the 2'(3')-monophosphates is now possible. In each series of 5'-mono-, di- and tri-phosphates or 2'(3')-monophosphates, the elution order is generally cytidine, uridine, guanosine and adenosine. By use of water instead of coating the resin with triethylammonium bicarbonate, the nucleotides and inorganic phosphate are found in the void volume; adenine is eluted very slowly, whereas adenosine is not eluted. Adenosine is eluted only with ethanol-water (1:3). The method is advantageous in that the recovery is quantitative, the buffer is easily removed, the capacity of the column is large (35 mugmoles pergram of resin), flow-rates are high, the time required is short and separations of combinations of inorganic phosphate, bases, nucleosides and nucleotides are now possible that previously could not be accomplished.", "contents": "Separation of nucleic acid hydrolysis products, purines, pyrimidines, nucleosides, nucleotides, ribonucleic acid hydrolyzates, and mixtures from nucleotide syntheses by column chromatography on amberlite XAD-4. Amberlite XAD-4 resin has been studied as a support for liquid-solid column chromatography. By coating the resin with triethylammonium bicarbonate, a new and unique separation of nucleic acid components has been achieved. Separations are accomplished with a linear gradient of this buffer from 0.1 to 0.4 M. Separation occurs in the following order: inorganic phosphate, purine or pyrimidine bases, 5'-monophosphates, nucleosides and 5'-diphosphates or 5'-triphosphates; the 2'(3')-monophosphates are eluted after either the 5'mono-, di-or triphosphates. The bases and nucleosides are separated in the order: cytosine, uracil, guanine and adenine. Inorganic phosphate and the nucleotides are eluted in the order: inorganic phosphate 5'-mono, di- and tri-phosphates. Excellent separation of the 5'-monophosphates and the 2'(3')-monophosphates is now possible. In each series of 5'-mono-, di- and tri-phosphates or 2'(3')-monophosphates, the elution order is generally cytidine, uridine, guanosine and adenosine. By use of water instead of coating the resin with triethylammonium bicarbonate, the nucleotides and inorganic phosphate are found in the void volume; adenine is eluted very slowly, whereas adenosine is not eluted. Adenosine is eluted only with ethanol-water (1:3). The method is advantageous in that the recovery is quantitative, the buffer is easily removed, the capacity of the column is large (35 mugmoles pergram of resin), flow-rates are high, the time required is short and separations of combinations of inorganic phosphate, bases, nucleosides and nucleotides are now possible that previously could not be accomplished.", "PMID": 956313}
{"id": "pubmed23n0032_2143", "title": "Gas-liquid chromatographic determination of tienilic acid (SKF 62.698) in human plasma and urine.", "content": "A gas-liquid chromatographic method is described for the determination of tienilic acid (SKF 62.698), a diuretic with uricosuric properties, in human  plasma and urine. The method, which is based on the methylation of the compound, is rapid, specific and sensitive. The lowest level accurately determined is about 50 ng/ml in plasma and 1 mug/ml in urine. The first results from a human volunteer are given.", "contents": "Gas-liquid chromatographic determination of tienilic acid (SKF 62.698) in human plasma and urine. A gas-liquid chromatographic method is described for the determination of tienilic acid (SKF 62.698), a diuretic with uricosuric properties, in human  plasma and urine. The method, which is based on the methylation of the compound, is rapid, specific and sensitive. The lowest level accurately determined is about 50 ng/ml in plasma and 1 mug/ml in urine. The first results from a human volunteer are given.", "PMID": 956314}
{"id": "pubmed23n0032_2144", "title": "[Duodeno-colic fistula due to cancer of the right colon].", "content": "The author presents a case of duodeno-colic fistula revealing carcinoma of the right hepatic flexure showing the difficulty of surgery. A right hemi-colectomy enlarged to the duodenum and pancreas is the ideal operation from a curative point of view, but rarely possible owing to the age of the patient and the constitutional background. The risk of breakdown of duodenal sutures, which is sometimes technically difficult after colonic removal, is far from negligible. The results of by-pass operations, with or without exclusion of the fistula, are poor and uncertain.", "contents": "[Duodeno-colic fistula due to cancer of the right colon]. The author presents a case of duodeno-colic fistula revealing carcinoma of the right hepatic flexure showing the difficulty of surgery. A right hemi-colectomy enlarged to the duodenum and pancreas is the ideal operation from a curative point of view, but rarely possible owing to the age of the patient and the constitutional background. The risk of breakdown of duodenal sutures, which is sometimes technically difficult after colonic removal, is far from negligible. The results of by-pass operations, with or without exclusion of the fistula, are poor and uncertain.", "PMID": 956307}
{"id": "pubmed23n0032_2145", "title": "Glycerolpropylsilane bonded phases in the steric exclusion chromatography of biological macromolecules.", "content": "Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18-19A while the amount of glycerol moiety varies from 80 to 150 mumoles/g depending on support pore diameter. It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.", "contents": "Glycerolpropylsilane bonded phases in the steric exclusion chromatography of biological macromolecules. Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18-19A while the amount of glycerol moiety varies from 80 to 150 mumoles/g depending on support pore diameter. It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.", "PMID": 956322}
{"id": "pubmed23n0032_2146", "title": "The determination of BHT in resmethrin by high-pressure liquid chromatography.", "content": "A method was developed to determine the amount of butylated hydroxy toluene in resmethrin and resmethrin formulations by high-pressure liquid chromatography.", "contents": "The determination of BHT in resmethrin by high-pressure liquid chromatography. A method was developed to determine the amount of butylated hydroxy toluene in resmethrin and resmethrin formulations by high-pressure liquid chromatography.", "PMID": 956323}
{"id": "pubmed23n0032_2147", "title": "Pyrolysis GLC identification of food and drug ingredients. I. Saccharin.", "content": "Pyrolysis gas-liquid chromatography, used in the qualitative analysis of saccharin, has provided reproducible pyrograms which permit direct characterization of these sweetners in a variety of commerical saccharin tablets and diet beverages. This method was used also to identify the salt, after conversion to the free imide, in a commercial multivitamin product. The tablet analysis required isolation by solvent extraction. Analyses were carried out on quantities ranging from 1 to 100 mug. The sensitivity of the method could be increased by addition of a pure sample of the compound sought to the unknown.", "contents": "Pyrolysis GLC identification of food and drug ingredients. I. Saccharin. Pyrolysis gas-liquid chromatography, used in the qualitative analysis of saccharin, has provided reproducible pyrograms which permit direct characterization of these sweetners in a variety of commerical saccharin tablets and diet beverages. This method was used also to identify the salt, after conversion to the free imide, in a commercial multivitamin product. The tablet analysis required isolation by solvent extraction. Analyses were carried out on quantities ranging from 1 to 100 mug. The sensitivity of the method could be increased by addition of a pure sample of the compound sought to the unknown.", "PMID": 956324}
{"id": "pubmed23n0032_2148", "title": "Separation of 5'-mono-, di- and triphosphates of 1-beta-D-arabinofuranosylcytosine and of 1-beta-D-arabinofuranosyluracil by thin-layer and high pressure liquid chromatography.", "content": "The separation of all the 5'-mono-, di- and triphosphates of ara-C and ara-U by thin-layer (TLC) and high pressure liquid chromatography (HPLC) is described. Whereas for the TLC two different systems are necessary, HPLC allows a rapid separation of all the nucleotides with high resolution, reproducibility, and sensitivity within 15 minutes. An example of an HPLC separation of ara-C and ara-U nucleotides derived from a biological sample is presented.", "contents": "Separation of 5'-mono-, di- and triphosphates of 1-beta-D-arabinofuranosylcytosine and of 1-beta-D-arabinofuranosyluracil by thin-layer and high pressure liquid chromatography. The separation of all the 5'-mono-, di- and triphosphates of ara-C and ara-U by thin-layer (TLC) and high pressure liquid chromatography (HPLC) is described. Whereas for the TLC two different systems are necessary, HPLC allows a rapid separation of all the nucleotides with high resolution, reproducibility, and sensitivity within 15 minutes. An example of an HPLC separation of ara-C and ara-U nucleotides derived from a biological sample is presented.", "PMID": 956325}
{"id": "pubmed23n0032_2149", "title": "Rapid, quantitative determination of tetrahydrocannabinol in marihuana by gas chromatography.", "content": "Using a simple injection adapter of our own design, the tetrahydrocannabinol content of marlhuana was determined by gas chromatography. The injection method obviates the need for preliminary extractions and allows analyses to be conducted on milligram quantities in less than thirty minutes.", "contents": "Rapid, quantitative determination of tetrahydrocannabinol in marihuana by gas chromatography. Using a simple injection adapter of our own design, the tetrahydrocannabinol content of marlhuana was determined by gas chromatography. The injection method obviates the need for preliminary extractions and allows analyses to be conducted on milligram quantities in less than thirty minutes.", "PMID": 956326}
{"id": "pubmed23n0032_2150", "title": "Structure determination of polyunsaturated fatty acids by gas chromatography-mass spectrometry-- a comparison of fragmentation patterns of various derivatives.", "content": "GC-MS has been applied to the characterization of mixtures of polyunsaturated fatty acids by way of their isopropylidene, alkylboronate, methyl ether, and trimethylsilyl ether derivatives. In our studies we found a convenient and efficient method for the oxidation of mixtures of polyunsaturated fatty acid methyl esters by osmium tetroxide. The resulting polyhdroxy compounds are easily converted into apolar derivatives for gas chromatographic separation. The mass spectra of these individual compounds were recorded as to their usefulness for complete structure elucidation. Only the trimethylsilyl ethers proved to yield all information necessary for determination of chain length as well as numbers and positions of double bonds of polyunsaturated fatty acids.", "contents": "Structure determination of polyunsaturated fatty acids by gas chromatography-mass spectrometry-- a comparison of fragmentation patterns of various derivatives. GC-MS has been applied to the characterization of mixtures of polyunsaturated fatty acids by way of their isopropylidene, alkylboronate, methyl ether, and trimethylsilyl ether derivatives. In our studies we found a convenient and efficient method for the oxidation of mixtures of polyunsaturated fatty acid methyl esters by osmium tetroxide. The resulting polyhdroxy compounds are easily converted into apolar derivatives for gas chromatographic separation. The mass spectra of these individual compounds were recorded as to their usefulness for complete structure elucidation. Only the trimethylsilyl ethers proved to yield all information necessary for determination of chain length as well as numbers and positions of double bonds of polyunsaturated fatty acids.", "PMID": 956327}
{"id": "pubmed23n0032_2151", "title": "Separation of benefin and trifluralin by gas chromatography using a liquid crystal column.", "content": "Benefin (N-[n-butyl]-N-ethyl-2,6-dinitro-alpha, alpha, alpha-trifluoro-p-toluidine) and trifluralin (2,6-dinitro-N,N-di-n-propyl-alpha, alpha, alpha-trifluoro-p-toluidine) (Figure 1) are isomeric preemergent herbicides (1,2). As they possess different activities, their use necessitates the development of a routine analytical method which differentiates between them. The separation of these isomers by gas-liquid chromatography (GLC) using conventional column packings has proved extremely difficult. Two partly successful separations have been previously reported to date. A partial separation was obtained using a Durapak-Carbowax 400/Poracil C column (3) and a complete separation using a column packed with a monomolecular layer of Carbowax 20M on Chromosorb W (4). Unfortunately, this latter column has a very limited life and the analysis time is 25 minutes. This paper reports a rapid and reproducible separation of benefin and trifluralin using a liquid crystal stationary phase.", "contents": "Separation of benefin and trifluralin by gas chromatography using a liquid crystal column. Benefin (N-[n-butyl]-N-ethyl-2,6-dinitro-alpha, alpha, alpha-trifluoro-p-toluidine) and trifluralin (2,6-dinitro-N,N-di-n-propyl-alpha, alpha, alpha-trifluoro-p-toluidine) (Figure 1) are isomeric preemergent herbicides (1,2). As they possess different activities, their use necessitates the development of a routine analytical method which differentiates between them. The separation of these isomers by gas-liquid chromatography (GLC) using conventional column packings has proved extremely difficult. Two partly successful separations have been previously reported to date. A partial separation was obtained using a Durapak-Carbowax 400/Poracil C column (3) and a complete separation using a column packed with a monomolecular layer of Carbowax 20M on Chromosorb W (4). Unfortunately, this latter column has a very limited life and the analysis time is 25 minutes. This paper reports a rapid and reproducible separation of benefin and trifluralin using a liquid crystal stationary phase.", "PMID": 956328}
{"id": "pubmed23n0032_2152", "title": "Intestinal metabolism of estrogens.", "content": "Estrogen metabolism in the human intestine was studied in two ways. Firstly, by measuring the excretion of 12 estrogens in pooled human late pregnancy feces before and during the administration of ampicillin (2 g/day). Secondly, by administering 5.4 and 20 mg of 16alpha-hydroxyestrone orally to two postmenopausal women and analyzing the estrogens in simultaneously drawn portal and peripheral venous blood samples at time intervals from 0 to 150 min after steroid administration. The majority of the estrogens in normal pregnancy feces were unconjugated. The amounts of estradiol, estreon and 16-epiestriol excreted, relative to the principal estrogen estriol, were greater than in pregnancy bile or urine and 16alpha-hydroxyestrone, an important biliary estrogen, was only present in trace amounts. Considerable quantities of 15alpha-hydroxyestradiol-17beta were also found. Ampicillin administration, which decreases intestinal bacterial steroid metabolism, caused a huge increase in the fecal excretion of conjugated estrogens. In particular it caused very striking increases in the excretion of both unconjugated and conjugated, estriol, 15alpha-hydroxyestrone, 15alpha-hydroxyestradiol and 2-methoxyestrone. These findings emphasize the active role played by the intestinal microflora in estrogen metabolism under normal conditions. Administration of 16alpha-hydroxyestrone resulted in increases in portal venous unconjugated and conjugated 16alpha-hydroxyestrone, 16-oxoestradiol-17beta, 15alpha-hydroxyestrone, 16-epiestriol and conjugated estriol levels. The most significant finding in both subjects was the large increase in portal venous unconjugated 15alpha-hydroxyestrone. This would suggest that the human intestine (or intestinal contents) has the ability to carry out the transformation, 16alpha-hydroxyestrone leads to 15alpha-hydroxyestrone. Increases in the same estrogens were found in peripheral plasma, with the increase in conjugated estriol occurring in peripheral blood before it was seen in portal blood. The largest elevations in peripheral plasma values were seen in unconjugated estriol and conjugated 16alpha-hydroxyestrone in the subject who received the 20 mg dose and in unconjugated 16alpha-hydroxyestrone and 16-oxoestradiol-17beta in the subject who had the 5.4 mg dose. The intestinal and enterohepatic metabolism of estrogens is discussed in relation to these findings.", "contents": "Intestinal metabolism of estrogens. Estrogen metabolism in the human intestine was studied in two ways. Firstly, by measuring the excretion of 12 estrogens in pooled human late pregnancy feces before and during the administration of ampicillin (2 g/day). Secondly, by administering 5.4 and 20 mg of 16alpha-hydroxyestrone orally to two postmenopausal women and analyzing the estrogens in simultaneously drawn portal and peripheral venous blood samples at time intervals from 0 to 150 min after steroid administration. The majority of the estrogens in normal pregnancy feces were unconjugated. The amounts of estradiol, estreon and 16-epiestriol excreted, relative to the principal estrogen estriol, were greater than in pregnancy bile or urine and 16alpha-hydroxyestrone, an important biliary estrogen, was only present in trace amounts. Considerable quantities of 15alpha-hydroxyestradiol-17beta were also found. Ampicillin administration, which decreases intestinal bacterial steroid metabolism, caused a huge increase in the fecal excretion of conjugated estrogens. In particular it caused very striking increases in the excretion of both unconjugated and conjugated, estriol, 15alpha-hydroxyestrone, 15alpha-hydroxyestradiol and 2-methoxyestrone. These findings emphasize the active role played by the intestinal microflora in estrogen metabolism under normal conditions. Administration of 16alpha-hydroxyestrone resulted in increases in portal venous unconjugated and conjugated 16alpha-hydroxyestrone, 16-oxoestradiol-17beta, 15alpha-hydroxyestrone, 16-epiestriol and conjugated estriol levels. The most significant finding in both subjects was the large increase in portal venous unconjugated 15alpha-hydroxyestrone. This would suggest that the human intestine (or intestinal contents) has the ability to carry out the transformation, 16alpha-hydroxyestrone leads to 15alpha-hydroxyestrone. Increases in the same estrogens were found in peripheral plasma, with the increase in conjugated estriol occurring in peripheral blood before it was seen in portal blood. The largest elevations in peripheral plasma values were seen in unconjugated estriol and conjugated 16alpha-hydroxyestrone in the subject who received the 20 mg dose and in unconjugated 16alpha-hydroxyestrone and 16-oxoestradiol-17beta in the subject who had the 5.4 mg dose. The intestinal and enterohepatic metabolism of estrogens is discussed in relation to these findings.", "PMID": 956337}
{"id": "pubmed23n0032_2153", "title": "Endocrine studies in a phenotypic girl with XY gonadal agenesis.", "content": "An 18-year-old phenotypic girl with XY gonadal agenesis had endocrine studies. The patient had a small amount of sexual hair, a slightly enlarged clitoris, normal labia and no posterior fusion. Her vagina was of normal length, but no cervix was palpable. At laparoscopy, no gonads, uterus or fallopian tubes were identified. The buccal smear was chromatin negative and the karyotype was 46XY. The 24 h LH plasma pattern was qualitatively similar to that found in normal late pubertal subjects, but the values were in the castrate range. The 24h mean plasma concentrations of testosterone and dihydrotestosterone were 16.4 ng/dl and 14.2 ng/dl, respectively. Urinary estrogen excretion was 15 and 19 mug/day. These clinical and endocrine findings suggest that the patient was a genetic male in whom Mullerian regression occurred normally; however, the testes probably ceased functioning early in gestation preventing normal development of the Wolffian system. This set of events resulted in agenesis of the gonaductal system and female external genitalia. The patient represents the second example of this variation of male pseudohermaphroditism associated with XY gonadal agenesis.", "contents": "Endocrine studies in a phenotypic girl with XY gonadal agenesis. An 18-year-old phenotypic girl with XY gonadal agenesis had endocrine studies. The patient had a small amount of sexual hair, a slightly enlarged clitoris, normal labia and no posterior fusion. Her vagina was of normal length, but no cervix was palpable. At laparoscopy, no gonads, uterus or fallopian tubes were identified. The buccal smear was chromatin negative and the karyotype was 46XY. The 24 h LH plasma pattern was qualitatively similar to that found in normal late pubertal subjects, but the values were in the castrate range. The 24h mean plasma concentrations of testosterone and dihydrotestosterone were 16.4 ng/dl and 14.2 ng/dl, respectively. Urinary estrogen excretion was 15 and 19 mug/day. These clinical and endocrine findings suggest that the patient was a genetic male in whom Mullerian regression occurred normally; however, the testes probably ceased functioning early in gestation preventing normal development of the Wolffian system. This set of events resulted in agenesis of the gonaductal system and female external genitalia. The patient represents the second example of this variation of male pseudohermaphroditism associated with XY gonadal agenesis.", "PMID": 956338}
{"id": "pubmed23n0032_2154", "title": "Serum thyroglobulin in inhabitants of an endemic goiter region of New Guinea.", "content": "Serum thyroglobulin Tg(RIA) was studied in 161 residents of the Nomane region of New Guinea. The incidence of antithyroglobulin antibodies (ATA) and antimicrosomal antibodies (anti-M) was also studied to assess the role of autoimmunity in goitrogenesis. ATA were detected in only 4 sera; these sera were excluded from the study since ATA interfere in the Tg radioimmunoassay. Anti-M antibodies were undetectable in all of 105 subjects whose sera were analyzed. Mean (+/-se) serum Tg(RIA) in the 84 nongoitrous (NG) subjects was 163.1 +/- 17.2 ng/ml, whereas that in 77 goitrous (G) subjects was 208.1 +/- 19.8 ng/ml; both values were much higher (P is less than 0.001) than that (5.1 +/- 0.49 ng/ml) in normal Californian subjects. The mean serum thyroid stimulating hormone (TSH) in the NG group (12.1 +/- 2.1 muU/ml) was not statistically different from that in the G group (10.1 +/- 1.5 muU/ml). Serum Tg(RIA) correlated positively with log TSH (r equals 0.38 P is less than 0.001). Intrigued by the finding of goiters in some residents of an endemic goiter region and its absence in other residents exposed to the same environmental factors, we evaluated the possibility that the thyroid glands of subjects who develop goiters may be inherently more responsive to any given level of TSH than those of the inhabitants without goiters. However, the slope of the correlation between serum Tg(RIA) and log TSH was only slightly (0.1 is less than P is greater than .05) higher in G than in the NG group. These studies suggested that factors other than thyroidal responsiveness to TSH must also be important in goiterogenesis of endemic goiter regions. We conclude that 1) serum thyroglobulin is a sensitive parameter of chronic as well as acute thyroidal stimulation; 2) Thyroid autoimmunity and increased thyroidal response to TSH do not explain goiterogenesis in New Guinea and attention should be focused on other possibilities.", "contents": "Serum thyroglobulin in inhabitants of an endemic goiter region of New Guinea. Serum thyroglobulin Tg(RIA) was studied in 161 residents of the Nomane region of New Guinea. The incidence of antithyroglobulin antibodies (ATA) and antimicrosomal antibodies (anti-M) was also studied to assess the role of autoimmunity in goitrogenesis. ATA were detected in only 4 sera; these sera were excluded from the study since ATA interfere in the Tg radioimmunoassay. Anti-M antibodies were undetectable in all of 105 subjects whose sera were analyzed. Mean (+/-se) serum Tg(RIA) in the 84 nongoitrous (NG) subjects was 163.1 +/- 17.2 ng/ml, whereas that in 77 goitrous (G) subjects was 208.1 +/- 19.8 ng/ml; both values were much higher (P is less than 0.001) than that (5.1 +/- 0.49 ng/ml) in normal Californian subjects. The mean serum thyroid stimulating hormone (TSH) in the NG group (12.1 +/- 2.1 muU/ml) was not statistically different from that in the G group (10.1 +/- 1.5 muU/ml). Serum Tg(RIA) correlated positively with log TSH (r equals 0.38 P is less than 0.001). Intrigued by the finding of goiters in some residents of an endemic goiter region and its absence in other residents exposed to the same environmental factors, we evaluated the possibility that the thyroid glands of subjects who develop goiters may be inherently more responsive to any given level of TSH than those of the inhabitants without goiters. However, the slope of the correlation between serum Tg(RIA) and log TSH was only slightly (0.1 is less than P is greater than .05) higher in G than in the NG group. These studies suggested that factors other than thyroidal responsiveness to TSH must also be important in goiterogenesis of endemic goiter regions. We conclude that 1) serum thyroglobulin is a sensitive parameter of chronic as well as acute thyroidal stimulation; 2) Thyroid autoimmunity and increased thyroidal response to TSH do not explain goiterogenesis in New Guinea and attention should be focused on other possibilities.", "PMID": 956339}
{"id": "pubmed23n0032_2155", "title": "Effects of phosphorus supplementation on serum parathyroid hormone and bone morphology in osteoporosis.", "content": "The effect of phosphorus (inorganic phosphate) supplementation was studied in seven postmenopausal women with osteoporosis. Prior to supplementation, all chemical parameters studied in serum and urine were normal. Bone density was below the fifth percentile for age in all but one patient, and the percentage of bone surface involved in resorption was higher than normal. During administration of the phosphorus supplement, fasting serum concentrations of calcium and immunoreactive parathyroid hormone showed no significant changes, while serum phosphorus, urinary calcium, and tubular reabsorption of phosphorus decreased. In four patients studied by balance techniques, calcium balance became positive or less negative. Bone-forming surface decreased and bone-resorbing surface increased in all patients. Bone-resorbing surface was highly correlated with total phosphorus intake. Density of the distal radius changed variably, while density of the midradius increased slightly in all patients.", "contents": "Effects of phosphorus supplementation on serum parathyroid hormone and bone morphology in osteoporosis. The effect of phosphorus (inorganic phosphate) supplementation was studied in seven postmenopausal women with osteoporosis. Prior to supplementation, all chemical parameters studied in serum and urine were normal. Bone density was below the fifth percentile for age in all but one patient, and the percentage of bone surface involved in resorption was higher than normal. During administration of the phosphorus supplement, fasting serum concentrations of calcium and immunoreactive parathyroid hormone showed no significant changes, while serum phosphorus, urinary calcium, and tubular reabsorption of phosphorus decreased. In four patients studied by balance techniques, calcium balance became positive or less negative. Bone-forming surface decreased and bone-resorbing surface increased in all patients. Bone-resorbing surface was highly correlated with total phosphorus intake. Density of the distal radius changed variably, while density of the midradius increased slightly in all patients.", "PMID": 956340}
{"id": "pubmed23n0032_2156", "title": "Plasma thyrotropin, thyroxine, and triiodothyronine relationships in man.", "content": "The physiologic relationships of plasma TSH, T4 and T3 levels measured every 20 min in seven healthy young men and one healthy young woman have been investigated. A nocturnal TSH surge was observed in all subjects on both nights of the 36-48 h baseline observation period. In males the maximum plasma TSH value occurred at 2300 h. The mean peak TSH level was 2.0 +/- 0.3 (se) muU/ml compared with a mean of 1.3 +/- 0.9 muU/ml for the entire baseline records of the 8 subjects. The effect of iv infusion of 32-1000 mug of somatostatin (SRIF) for 1 1/2-3 h was investigated in four of the male subjects during 2 or 4 consecutive nights following the control period. Temporal relationships between the hormonal fluctuations observed throughout the control period and during the nights of SRIF infusion were investigated using time series analysis and Student's t test. Rapid fluctuations of plasma T4 and T3 concentration were noted, even when corrected for changes in total protein concentration, with an average coefficient of variation of 10% for T3 and 12% for T4. No increment of plasma T4 or T3 followed the nocturnal TSH surge nor were the rapid fluctuations of the thyroid hormones altered by the TSH surge. SRIF infusion commencing at 2300 h suppressed the elevated TSH levels (P is less than 0.01) while similar infusions begun at 2100 h blocked the expected nocturnal TSH rise observed during control periods in male subjects. Plasma T4 and T3 levels were not significantly affected by the administration of SRIF. The relationship of the rapid plasma T4 and T3 variations to postural changes was investigated in four euthyroid male subjects. Serum levels of TSH, T4 and T3 and total protein were determined at 15 min intervals while postural changes were carefully monitored. The ratios of T4 and T3 to total protein were relatively stable (3-4% coefficient of variation) when the subjects were kept in a supine and motionless position. A 50 mug bolus infusion of T4 raised the basal T4 level by only 1-2 mug/dl. The data suggest that short-term fluctuation of plasma T4 and T3 result from changes in protein concentration due to hemodynamic responses to alteration of posture and physical activity and not to pulsatile secretion of T4 and T3.", "contents": "Plasma thyrotropin, thyroxine, and triiodothyronine relationships in man. The physiologic relationships of plasma TSH, T4 and T3 levels measured every 20 min in seven healthy young men and one healthy young woman have been investigated. A nocturnal TSH surge was observed in all subjects on both nights of the 36-48 h baseline observation period. In males the maximum plasma TSH value occurred at 2300 h. The mean peak TSH level was 2.0 +/- 0.3 (se) muU/ml compared with a mean of 1.3 +/- 0.9 muU/ml for the entire baseline records of the 8 subjects. The effect of iv infusion of 32-1000 mug of somatostatin (SRIF) for 1 1/2-3 h was investigated in four of the male subjects during 2 or 4 consecutive nights following the control period. Temporal relationships between the hormonal fluctuations observed throughout the control period and during the nights of SRIF infusion were investigated using time series analysis and Student's t test. Rapid fluctuations of plasma T4 and T3 concentration were noted, even when corrected for changes in total protein concentration, with an average coefficient of variation of 10% for T3 and 12% for T4. No increment of plasma T4 or T3 followed the nocturnal TSH surge nor were the rapid fluctuations of the thyroid hormones altered by the TSH surge. SRIF infusion commencing at 2300 h suppressed the elevated TSH levels (P is less than 0.01) while similar infusions begun at 2100 h blocked the expected nocturnal TSH rise observed during control periods in male subjects. Plasma T4 and T3 levels were not significantly affected by the administration of SRIF. The relationship of the rapid plasma T4 and T3 variations to postural changes was investigated in four euthyroid male subjects. Serum levels of TSH, T4 and T3 and total protein were determined at 15 min intervals while postural changes were carefully monitored. The ratios of T4 and T3 to total protein were relatively stable (3-4% coefficient of variation) when the subjects were kept in a supine and motionless position. A 50 mug bolus infusion of T4 raised the basal T4 level by only 1-2 mug/dl. The data suggest that short-term fluctuation of plasma T4 and T3 result from changes in protein concentration due to hemodynamic responses to alteration of posture and physical activity and not to pulsatile secretion of T4 and T3.", "PMID": 956341}
{"id": "pubmed23n0032_2157", "title": "Hypothyroid-like alterations in testosterone metabolism in anorexia nervosa.", "content": "The metabolism of 14C-testosterone was studied in 8 severely underweight young women with anorexia nervosa. The urinary androsterone/etiocholanolone (A/E) ratio was uniformly low, in a range characteristic of hypothyroidism; the patients also showed low plasma concentrations of triiodothyronine (T3). Clinical remission as manifested by weight gain was accompanied by concomitant increases of the A/E ratio and the plasma T3 concentration to or toward normal. The administration of  T3 also resulted in a shift of the A/E ratio toward normal. These data demonstrate that the low plasma T3 concentrations in patients with anorexia nervosa may be related to the development of one of the characteristic biochemical abnormalities found in clinical hypothyroidism, namely a decreased A/E ratio. These data suggest that the \"low T3 syndrome\" may be associated with biochemical hypothyroidism.", "contents": "Hypothyroid-like alterations in testosterone metabolism in anorexia nervosa. The metabolism of 14C-testosterone was studied in 8 severely underweight young women with anorexia nervosa. The urinary androsterone/etiocholanolone (A/E) ratio was uniformly low, in a range characteristic of hypothyroidism; the patients also showed low plasma concentrations of triiodothyronine (T3). Clinical remission as manifested by weight gain was accompanied by concomitant increases of the A/E ratio and the plasma T3 concentration to or toward normal. The administration of  T3 also resulted in a shift of the A/E ratio toward normal. These data demonstrate that the low plasma T3 concentrations in patients with anorexia nervosa may be related to the development of one of the characteristic biochemical abnormalities found in clinical hypothyroidism, namely a decreased A/E ratio. These data suggest that the \"low T3 syndrome\" may be associated with biochemical hypothyroidism.", "PMID": 956342}
{"id": "pubmed23n0032_2158", "title": "Effect of the antihistaminic agents meclastine and dexchlorpheniramine on the response of human growth hormone to arginine infusion and insulin hypoglycemia.", "content": "Histamine is found in most tissues including the central nervous system. Here it reaches the highest concentrations in the hypothalamus and in the median eminence. In order to evaluate the possible role of endogenous histamine in the control of human growth hormone (hGH) secretion, we investigated the effect of two anti-histamine drugs, meclastine and dexchlorpheniramine, on the hGH response to arginine infusion and to insulin hypoglycemia in 30 normal subjects. The oral administration of meclastine for three days or the intravenous infusion of dexchlorpheniramine significantly reduced the hGH response to arginine infusion. Neither drug affected the secretion of hGH following insulin hypoglycemia. These results suggest that histamine is involved in the control of hGH release, at least in response to arginine. Our data are consistent with the finding that histamine stimulates GH release in the baboon (Meyer and Knobil, Endocrinology 80: 163, 1967) and with previous results indicating that the release of hGH in response to different stimuli is subject to different regulatory mechanisms.", "contents": "Effect of the antihistaminic agents meclastine and dexchlorpheniramine on the response of human growth hormone to arginine infusion and insulin hypoglycemia. Histamine is found in most tissues including the central nervous system. Here it reaches the highest concentrations in the hypothalamus and in the median eminence. In order to evaluate the possible role of endogenous histamine in the control of human growth hormone (hGH) secretion, we investigated the effect of two anti-histamine drugs, meclastine and dexchlorpheniramine, on the hGH response to arginine infusion and to insulin hypoglycemia in 30 normal subjects. The oral administration of meclastine for three days or the intravenous infusion of dexchlorpheniramine significantly reduced the hGH response to arginine infusion. Neither drug affected the secretion of hGH following insulin hypoglycemia. These results suggest that histamine is involved in the control of hGH release, at least in response to arginine. Our data are consistent with the finding that histamine stimulates GH release in the baboon (Meyer and Knobil, Endocrinology 80: 163, 1967) and with previous results indicating that the release of hGH in response to different stimuli is subject to different regulatory mechanisms.", "PMID": 956343}
{"id": "pubmed23n0032_2159", "title": "Reversal of decreased human adipose tissue lipoprotein lipase and hypertriglyceridemia after treatment of hypothyroidism.", "content": "To determine whether adipose tissue lipoprotein lipase (LPL) plays a role in the regulation of triglyceride (TG) metabolism in hypothyroidism, the activity of the enzyme was measured in the subcutaneous adipose tissue of six hypothyroid patients before and during therapy with L-thyroxine. The activity of the activated form of the enzyme, measured as heparin elutable LPL, was lower in hypothyroid patients (1.54 +/- 0.93; mU/10(6) cells; mean +/- SD) than in controls (3.26 +/- 1.49; P less than .02) and increased (163 +/- 89%; P less than .01) with treatment to levels comparable to the controls. The total activity of LPL, measured in ammonium hydroxide extracts of acetone ether tissue powders, was in the low normal range in the hypothyroid patients (0.68 +/- 0.42), but not significantly different from normal (1.10 +/- 0.58) and did not increase significantly (92 +/- 105%), with treatment. Plasma post heparin lipolytic activity (PHLA) was low in hypothyroidism and increased (111 +/- 78%; P less than .05) with treatment. These increases in PHLA correlated with the increases in the activity of heparin elutable LPL (r = .88, P less than .05). In all patients fasting plasma TG levels decreased (-43 +/- 25%; P less than .02) after treatment. Serial determination of heparin elutable LPL activity, PHLA, and plasma TG during L-thyroxine treatment revealed a correlation between the per cent changes in PHLA and heparin elutable LPL activity (r = .68, P less than .05), an inverse correlation between plasma TG levels and heparin elutable LPL (r = -0.53,P less than .05) and no correlation between plasma TG and PHLA (r = -0.05). These results suggest that the low PHLA and hypertriglyceridemia of hypothyroidism are related to low adipose tissue LPL activity. All these parameters return to normal after treatment with L-thyroxine and attainment of euthyroidism.", "contents": "Reversal of decreased human adipose tissue lipoprotein lipase and hypertriglyceridemia after treatment of hypothyroidism. To determine whether adipose tissue lipoprotein lipase (LPL) plays a role in the regulation of triglyceride (TG) metabolism in hypothyroidism, the activity of the enzyme was measured in the subcutaneous adipose tissue of six hypothyroid patients before and during therapy with L-thyroxine. The activity of the activated form of the enzyme, measured as heparin elutable LPL, was lower in hypothyroid patients (1.54 +/- 0.93; mU/10(6) cells; mean +/- SD) than in controls (3.26 +/- 1.49; P less than .02) and increased (163 +/- 89%; P less than .01) with treatment to levels comparable to the controls. The total activity of LPL, measured in ammonium hydroxide extracts of acetone ether tissue powders, was in the low normal range in the hypothyroid patients (0.68 +/- 0.42), but not significantly different from normal (1.10 +/- 0.58) and did not increase significantly (92 +/- 105%), with treatment. Plasma post heparin lipolytic activity (PHLA) was low in hypothyroidism and increased (111 +/- 78%; P less than .05) with treatment. These increases in PHLA correlated with the increases in the activity of heparin elutable LPL (r = .88, P less than .05). In all patients fasting plasma TG levels decreased (-43 +/- 25%; P less than .02) after treatment. Serial determination of heparin elutable LPL activity, PHLA, and plasma TG during L-thyroxine treatment revealed a correlation between the per cent changes in PHLA and heparin elutable LPL activity (r = .68, P less than .05), an inverse correlation between plasma TG levels and heparin elutable LPL (r = -0.53,P less than .05) and no correlation between plasma TG and PHLA (r = -0.05). These results suggest that the low PHLA and hypertriglyceridemia of hypothyroidism are related to low adipose tissue LPL activity. All these parameters return to normal after treatment with L-thyroxine and attainment of euthyroidism.", "PMID": 956344}
{"id": "pubmed23n0032_2160", "title": "Thyroid-pituitary feedback during iodine repletion.", "content": "The changes in serum triiodothyronine (T3), thyroxine (T4), and thyrotropin (TSH) were measured during iodine repletion in a woman who was severely iodine-deficient because of a congenital iodide-trapping defect. Serum T3 became detectable 12 h after iodine was begun and reached 68 ng/dl, a level within the normal range, by 36 h. It rose progressively during the first 9 days reaching a supra-normal level (200 ng/dl) and then fell slowly to 130 ng/dl. Serum T4 was not detected (less than 1 mug/dl) until the 9th day and then rose to 6.8 mug/dl. Serum TSH fell rapidly during the first 9 days (disappearance rate was 0.17/day); there was a significant negative correlation with serum T3 (P less than 0.01). From the 10th through the 32nd day serum TSH fell more slowly (disappearance rate was 0.05/day) and correlation was with serum T4 (P less than 0.001), not T3. It appears that either T3 or T4 may regulate TSH secretion by direct effects on the thyrotroph, although their intracellular mode of action has not been defined.", "contents": "Thyroid-pituitary feedback during iodine repletion. The changes in serum triiodothyronine (T3), thyroxine (T4), and thyrotropin (TSH) were measured during iodine repletion in a woman who was severely iodine-deficient because of a congenital iodide-trapping defect. Serum T3 became detectable 12 h after iodine was begun and reached 68 ng/dl, a level within the normal range, by 36 h. It rose progressively during the first 9 days reaching a supra-normal level (200 ng/dl) and then fell slowly to 130 ng/dl. Serum T4 was not detected (less than 1 mug/dl) until the 9th day and then rose to 6.8 mug/dl. Serum TSH fell rapidly during the first 9 days (disappearance rate was 0.17/day); there was a significant negative correlation with serum T3 (P less than 0.01). From the 10th through the 32nd day serum TSH fell more slowly (disappearance rate was 0.05/day) and correlation was with serum T4 (P less than 0.001), not T3. It appears that either T3 or T4 may regulate TSH secretion by direct effects on the thyrotroph, although their intracellular mode of action has not been defined.", "PMID": 956345}
{"id": "pubmed23n0032_2161", "title": "Blood pressure fall by angiotensin II antagonist in patients with Bartter's syndrome.", "content": "Intravenous infusion of 600 ng/kg/min of 1-sarcosine, 8-isoleucine-angiotensin II, an angiotensin II antagonist, caused a marked blood pressure fall and a decrease in plasma aldosterone in 3 patients with Bartter's syndrome. These results indicate that proximal cause of Bartter's syndrome is an arteriolar hyporesponsiveness to angiotensin II and that this angiotensin II analogue has an antagonist activity on peripheral arterioles as well as adrenal cortex.", "contents": "Blood pressure fall by angiotensin II antagonist in patients with Bartter's syndrome. Intravenous infusion of 600 ng/kg/min of 1-sarcosine, 8-isoleucine-angiotensin II, an angiotensin II antagonist, caused a marked blood pressure fall and a decrease in plasma aldosterone in 3 patients with Bartter's syndrome. These results indicate that proximal cause of Bartter's syndrome is an arteriolar hyporesponsiveness to angiotensin II and that this angiotensin II analogue has an antagonist activity on peripheral arterioles as well as adrenal cortex.", "PMID": 956346}
{"id": "pubmed23n0032_2162", "title": "Growth hormone, prolactin and chorionic somatomammotropin in normal and molar pregnancy.", "content": "Twenty patients with molar pregnancy, ten normal pregnant women and ten healthy non-pregnant women were given 30 g of arginine intravenously. The serum concentration of growth hormone, prolactin and chorionic somatomammotropin (CS) was determined by radioimmunoassay. In addition, serum 17beta-estradiol, estriol and progesterone were also measured. Arginine infusion induced a sharp rise of GH in patients with molar pregnancy and in nonpregnant subjects, but the response in normal pregnancy was blunted. The response of PRL was high in patients with molar pregnancy, blunted in normal pregnancy and very small in nonpregnant subjects. CS did not respond at all to arginine infusion both in normal pregnancy and molar pregnancy. The high response to argine of PRL, normal response of GH and low baseline secretion and no response of CS may be characteristic of molar pregnancy.", "contents": "Growth hormone, prolactin and chorionic somatomammotropin in normal and molar pregnancy. Twenty patients with molar pregnancy, ten normal pregnant women and ten healthy non-pregnant women were given 30 g of arginine intravenously. The serum concentration of growth hormone, prolactin and chorionic somatomammotropin (CS) was determined by radioimmunoassay. In addition, serum 17beta-estradiol, estriol and progesterone were also measured. Arginine infusion induced a sharp rise of GH in patients with molar pregnancy and in nonpregnant subjects, but the response in normal pregnancy was blunted. The response of PRL was high in patients with molar pregnancy, blunted in normal pregnancy and very small in nonpregnant subjects. CS did not respond at all to arginine infusion both in normal pregnancy and molar pregnancy. The high response to argine of PRL, normal response of GH and low baseline secretion and no response of CS may be characteristic of molar pregnancy.", "PMID": 956347}
{"id": "pubmed23n0032_2163", "title": "Cortisol-induced suppression of plasma testosterone in normal adult males.", "content": "The response of plasma testosterone, LH and FSH to oral administration of cortisol or dexamethasone was studied for 26 h in 6 groups of 12 normal adult males. Twenty-four hour treatment with cortisol (60 mg at 1000 h, followed by 30 mg at 2 h intervals) or with dexamethasone (6 mg at 1000 h, followed by 3 mg at 6 h intervals) was without effect on testosterone during the day but suppressed the nocturnal rise completely. LH and FSH did not decrease at any time during the day and showed a highly significant increase during the second part of the night. Short-term treatment (1000-1600 h, 1600-2200 h or 2400 h-0800 h) with cortisol was less effective in suppressing the nocturnal rise in testosterone. It was concluded that administration of cortisol leading to plasma levels as seen under treatment with ACTH suppresses testosterone by abolishing or flattening the nocturnal rise. This effect was not mediated by changes in LH or FSH. Our data suggest that the ACTH-induced suppression of testosterone is due to an action of cortisol.", "contents": "Cortisol-induced suppression of plasma testosterone in normal adult males. The response of plasma testosterone, LH and FSH to oral administration of cortisol or dexamethasone was studied for 26 h in 6 groups of 12 normal adult males. Twenty-four hour treatment with cortisol (60 mg at 1000 h, followed by 30 mg at 2 h intervals) or with dexamethasone (6 mg at 1000 h, followed by 3 mg at 6 h intervals) was without effect on testosterone during the day but suppressed the nocturnal rise completely. LH and FSH did not decrease at any time during the day and showed a highly significant increase during the second part of the night. Short-term treatment (1000-1600 h, 1600-2200 h or 2400 h-0800 h) with cortisol was less effective in suppressing the nocturnal rise in testosterone. It was concluded that administration of cortisol leading to plasma levels as seen under treatment with ACTH suppresses testosterone by abolishing or flattening the nocturnal rise. This effect was not mediated by changes in LH or FSH. Our data suggest that the ACTH-induced suppression of testosterone is due to an action of cortisol.", "PMID": 956348}
{"id": "pubmed23n0032_2164", "title": "FSH-secreting pituitary adenomas: stimulation and suppression studies in two patients.", "content": "Two patients with pituitary adenomas, partial hypopituitarism and abnormally high serum concentrations of FSH are described. In neither patient was FSH increased after the administration of LH releasing hormone and in one patient tested, there was no FSH response to clomiphene citrate. The decrease in serum FSH in response to high concentrations of testosterone (greater than 1100 ng/dl) was less than in normal men. These results indicate partial autonomy of FSH producing adenomas. Since the elevated serum FSH produced no obvious clinical symptoms, it may be undetected unless serum FSH concentrations are measured routinely in patients with pituitary tumors.", "contents": "FSH-secreting pituitary adenomas: stimulation and suppression studies in two patients. Two patients with pituitary adenomas, partial hypopituitarism and abnormally high serum concentrations of FSH are described. In neither patient was FSH increased after the administration of LH releasing hormone and in one patient tested, there was no FSH response to clomiphene citrate. The decrease in serum FSH in response to high concentrations of testosterone (greater than 1100 ng/dl) was less than in normal men. These results indicate partial autonomy of FSH producing adenomas. Since the elevated serum FSH produced no obvious clinical symptoms, it may be undetected unless serum FSH concentrations are measured routinely in patients with pituitary tumors.", "PMID": 956349}
{"id": "pubmed23n0032_2165", "title": "Effects of dopamine infusion on pituitary hormone secretion in humans.", "content": "Dopamine, infused at a rate of 4 mug/kg/min for 3-4 h unaccompanied by any significant changes in cardiovascular dynamics, induced a prompt and sustained suppression of circulating prolactin (PRL) levels in normal men and women as well as in patients with hyperprolactinemia. At the discontinuation of the infusion, there was a marked rebound in PRL levels in normal subjects and a rapid return to basal levels in hyperprolactinemic patients. Dopamine infusion also induced a significant fall in LH levels in the normal subjects with a marked rebound in LH levels following the infusion. No significant changes in GH, TSH, and FSH levels were observed. These data indicate that in man a dopaminergic mechanism ixists in the regulation of PRL secretion and that dopamine also exerts an inhibitory effect of LH release.", "contents": "Effects of dopamine infusion on pituitary hormone secretion in humans. Dopamine, infused at a rate of 4 mug/kg/min for 3-4 h unaccompanied by any significant changes in cardiovascular dynamics, induced a prompt and sustained suppression of circulating prolactin (PRL) levels in normal men and women as well as in patients with hyperprolactinemia. At the discontinuation of the infusion, there was a marked rebound in PRL levels in normal subjects and a rapid return to basal levels in hyperprolactinemic patients. Dopamine infusion also induced a significant fall in LH levels in the normal subjects with a marked rebound in LH levels following the infusion. No significant changes in GH, TSH, and FSH levels were observed. These data indicate that in man a dopaminergic mechanism ixists in the regulation of PRL secretion and that dopamine also exerts an inhibitory effect of LH release.", "PMID": 956350}
{"id": "pubmed23n0032_2166", "title": "A radioimmunoassay for measurement of 3,3'-L-diiodothyronine (T2).", "content": "A sensitive, specific and reproducible radioimmunoassay (RIA) for measurement of 3,3'-L-diiodothyoride (T2) in concentrated ethanol extracts of serum and amniotic fluid is described.  The T2 binding antiserum was prepared aby immunization of rabbits with T2-human serum albumin conjugate.  Of various thyroid hormone derivatives tested, only 3-L-monoiodothyonine cross-reacted significantly with T2 binding sites on the antiserum...", "contents": "A radioimmunoassay for measurement of 3,3'-L-diiodothyronine (T2). A sensitive, specific and reproducible radioimmunoassay (RIA) for measurement of 3,3'-L-diiodothyoride (T2) in concentrated ethanol extracts of serum and amniotic fluid is described.  The T2 binding antiserum was prepared aby immunization of rabbits with T2-human serum albumin conjugate.  Of various thyroid hormone derivatives tested, only 3-L-monoiodothyonine cross-reacted significantly with T2 binding sites on the antiserum...", "PMID": 956351}
{"id": "pubmed23n0032_2167", "title": "17-Deoxygenation: a new pathway of cortisol metabolism. Isolation of 17-deoxycortolonic acids.", "content": "The existence of a 17-deoxylation pathway in the metabolism of cortisol has been established by isolation of two 17-deoxy metabolites of cortisol. They have been identified as the 20alpha and 20beta-hydroxy isomers of 3alpha,20-dihydroxy, 11-oxo-5beta-pregnan-21-oic acids by comparison of the nuclear magnetic resonance spectra (nmr) and high resolution mass spectra of their methyl esters with authentic samples and confirmed by reverse isotope dilution.", "contents": "17-Deoxygenation: a new pathway of cortisol metabolism. Isolation of 17-deoxycortolonic acids. The existence of a 17-deoxylation pathway in the metabolism of cortisol has been established by isolation of two 17-deoxy metabolites of cortisol. They have been identified as the 20alpha and 20beta-hydroxy isomers of 3alpha,20-dihydroxy, 11-oxo-5beta-pregnan-21-oic acids by comparison of the nuclear magnetic resonance spectra (nmr) and high resolution mass spectra of their methyl esters with authentic samples and confirmed by reverse isotope dilution.", "PMID": 956352}
{"id": "pubmed23n0032_2168", "title": "Effect of sulpiride-induced hyperprolactinemia on serum testosterone response to HCG in normal men.", "content": "In six normal volunteers hyperprolactinemia was induced by sulpiride (150 mg/day) for 10 days. Both before and during sulpiride hCG was injected; the higher testosterone response to hCG, when PRL levels were enhanced, suggests a possible stimulatory role of PRL on Leydig cells.", "contents": "Effect of sulpiride-induced hyperprolactinemia on serum testosterone response to HCG in normal men. In six normal volunteers hyperprolactinemia was induced by sulpiride (150 mg/day) for 10 days. Both before and during sulpiride hCG was injected; the higher testosterone response to hCG, when PRL levels were enhanced, suggests a possible stimulatory role of PRL on Leydig cells.", "PMID": 956353}
{"id": "pubmed23n0032_2169", "title": "Measurement of glucose turnover in the human newborn with glucose-1-13C.", "content": "Systemic glucose production rates were measured in 6 normal newborn infants by dilution of glucose-1-13C tracer according to the prime constant-rate infusion technique. Glucose production rates were 4.4 +/- 0.39 mg/kg. min. (Mean +/- S.D.) in 4 infants at 2 hours of age, and were 3.83 and 3.86 mg/kg. min. in 2 infants at 1 day of age. Systemic glucose production accounts for 50% of the substrate utilized for oxidative metabolism in newborn infants.", "contents": "Measurement of glucose turnover in the human newborn with glucose-1-13C. Systemic glucose production rates were measured in 6 normal newborn infants by dilution of glucose-1-13C tracer according to the prime constant-rate infusion technique. Glucose production rates were 4.4 +/- 0.39 mg/kg. min. (Mean +/- S.D.) in 4 infants at 2 hours of age, and were 3.83 and 3.86 mg/kg. min. in 2 infants at 1 day of age. Systemic glucose production accounts for 50% of the substrate utilized for oxidative metabolism in newborn infants.", "PMID": 956354}
{"id": "pubmed23n0032_2170", "title": "Competition by 24,25-dihydroxycholecalciferol in the competitive protein binding radioassay of 25-hydroxycalciferol.", "content": "24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25-hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD. Serum 24,25-(OH)2D content was measured following 24,25-(OH)2D3 isolation from lipid extracts by liquid-gel partition chromatography. Since normal serum 24,25-(OH)2D concentration is appreciable, non-chromatographic 25-OHD assays most probably overestimate serum 25-OHD levels as a result of their recognition of 24,25-(OH)2D3.", "contents": "Competition by 24,25-dihydroxycholecalciferol in the competitive protein binding radioassay of 25-hydroxycalciferol. 24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25-hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD. Serum 24,25-(OH)2D content was measured following 24,25-(OH)2D3 isolation from lipid extracts by liquid-gel partition chromatography. Since normal serum 24,25-(OH)2D concentration is appreciable, non-chromatographic 25-OHD assays most probably overestimate serum 25-OHD levels as a result of their recognition of 24,25-(OH)2D3.", "PMID": 956355}
{"id": "pubmed23n0032_2171", "title": "Effect of delay on culture of urine.", "content": "One hundred random urine specimens which were submitted for culture were planted using a calibrated loop within 2 h of collection and 2 and 4 h later after standing at room temperature. Colonies were counted after an 18- to 24-h incubation at 37 degrees C. Fifteen of the specimens demonstrated increases in counts exceeding 1 X log10; four increased from less than 10(5)/ml to greater than 10(5)/ml (three by the 4-h culture and a fourth by the 6-h culture), supporting the concept that delays of greater than 2 h in inoculating cultures may produce results which could cause errors in diagnosis.", "contents": "Effect of delay on culture of urine. One hundred random urine specimens which were submitted for culture were planted using a calibrated loop within 2 h of collection and 2 and 4 h later after standing at room temperature. Colonies were counted after an 18- to 24-h incubation at 37 degrees C. Fifteen of the specimens demonstrated increases in counts exceeding 1 X log10; four increased from less than 10(5)/ml to greater than 10(5)/ml (three by the 4-h culture and a fourth by the 6-h culture), supporting the concept that delays of greater than 2 h in inoculating cultures may produce results which could cause errors in diagnosis.", "PMID": 956356}
{"id": "pubmed23n0032_2172", "title": "Simplified method for efficient intravascular inoculation of chicken embryos.", "content": "The simple syringe-stabilizer unit described in this note provides a means for rapid intravascular inoculation of embryonated chicken eggs with minimal embryonic death from vascular trauma.", "contents": "Simplified method for efficient intravascular inoculation of chicken embryos. The simple syringe-stabilizer unit described in this note provides a means for rapid intravascular inoculation of embryonated chicken eggs with minimal embryonic death from vascular trauma.", "PMID": 956357}
{"id": "pubmed23n0032_2173", "title": "Differential recovery of Streptococcus mutans from various mitis-salivarius agar preparations.", "content": "Recoveries of Streptococcus mutans from human dental plaque were lower when plated on mitis-salivarius agar obtained from Baltimore Biological Laboratories as compared with mitis-salivarius agar obtained from Difco Laboratories. However, no difference in recoveries of established laboratory strains of S. mutans was observed between these two agar preparations.", "contents": "Differential recovery of Streptococcus mutans from various mitis-salivarius agar preparations. Recoveries of Streptococcus mutans from human dental plaque were lower when plated on mitis-salivarius agar obtained from Baltimore Biological Laboratories as compared with mitis-salivarius agar obtained from Difco Laboratories. However, no difference in recoveries of established laboratory strains of S. mutans was observed between these two agar preparations.", "PMID": 956358}
{"id": "pubmed23n0032_2174", "title": "Evaluation of a hypertonic sucrose medium for the detection of fungi in blood cultures.", "content": "A comparison was made between biphasic brain heart infusion medium without and with 15% sucrose in vented blood culture bottles. A higher recovery rate (P less than 0.01) of fungi was noted in the medium without sucrose.", "contents": "Evaluation of a hypertonic sucrose medium for the detection of fungi in blood cultures. A comparison was made between biphasic brain heart infusion medium without and with 15% sucrose in vented blood culture bottles. A higher recovery rate (P less than 0.01) of fungi was noted in the medium without sucrose.", "PMID": 956359}
{"id": "pubmed23n0032_2175", "title": "Virological and serological studies of Venezuelan equine encephalomyelitis in humans.", "content": "During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illness in 50 (75%) of the confirmed cases. Serological confirmation was obtained in 17 cases (25%). Virus isolations were most often obtained from sera collected during the first 3 days of illness. Peak serum virus titers (algebraic mean, 10(5-7) suckling mouse intracranial 50% lethal doses [SMICLD50] per ml) occurred on day 2 of illness. One-half of the sera from which virus was isolated contained at least 10(5) SMICLD50/ml, which has been shown to be sufficient to infect some vector mosquitoes. Blood from 13 virus-positive VEE cases was obtained 1 and 11 months after illness. Hemagglutination-inhibiting, complement-fixing, and neutralizing antibodies were formed by all 13 patients 1 month after illness. Hemagglutination-inhibiting antibody titers were essentially unchanged 11 months after illness. Complement-fixing antibody was undetectable 11 months after illness in 23% of cases and was detectable at dilutions of 1:8 or 1:6 in 77%. Neutralizing antibody (measured by log neutralization index) was not detectable 1 year after illness in one person (8%); titers had declined from 1.0 to 2.0 in 46%, were unchanged in 39%, and were not tested in one person (8%). No evidence of intrafamilial spread of VEE virus was obtained in either of two illness and antibody surveys. A randomized household illness and antibody survey of 681 Port Isabel residents revealed an inapparent infection ratio of 1:11 and an overall antibody prevalence of 3.2%.", "contents": "Virological and serological studies of Venezuelan equine encephalomyelitis in humans. During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illness in 50 (75%) of the confirmed cases. Serological confirmation was obtained in 17 cases (25%). Virus isolations were most often obtained from sera collected during the first 3 days of illness. Peak serum virus titers (algebraic mean, 10(5-7) suckling mouse intracranial 50% lethal doses [SMICLD50] per ml) occurred on day 2 of illness. One-half of the sera from which virus was isolated contained at least 10(5) SMICLD50/ml, which has been shown to be sufficient to infect some vector mosquitoes. Blood from 13 virus-positive VEE cases was obtained 1 and 11 months after illness. Hemagglutination-inhibiting, complement-fixing, and neutralizing antibodies were formed by all 13 patients 1 month after illness. Hemagglutination-inhibiting antibody titers were essentially unchanged 11 months after illness. Complement-fixing antibody was undetectable 11 months after illness in 23% of cases and was detectable at dilutions of 1:8 or 1:6 in 77%. Neutralizing antibody (measured by log neutralization index) was not detectable 1 year after illness in one person (8%); titers had declined from 1.0 to 2.0 in 46%, were unchanged in 39%, and were not tested in one person (8%). No evidence of intrafamilial spread of VEE virus was obtained in either of two illness and antibody surveys. A randomized household illness and antibody survey of 681 Port Isabel residents revealed an inapparent infection ratio of 1:11 and an overall antibody prevalence of 3.2%.", "PMID": 956360}
{"id": "pubmed23n0032_2176", "title": "Comparison of three methods for grouping streptococci.", "content": "Two new methods for serological grouping of beta-hemolytic streptococci, the nitrous acid extraction procedure of El Kholy et al. and the slide agglutination method of Christensen et al., were compared with the Lancefield hot-hydrochloric acid extraction method in classifying 92 strains of groups A, B, C, and G. The nitrous acid extraction method was easily performed, specific, and sensitive when highly potent antisera were used. For the Christensen method these highly potent antisera had to be diluted to avoid cross-reactions between groups A and C and groups B and G, respectively. A few strains, most of them group B, could not be grouped by the latter method. Using these three grouping methods, two sets of commercial sera were compared with the more potent sera supplied by R. C. Lancefield. The low antibody content of these commercial sera, especially anti-group B and G sera, contributed to the inferior results obtained in some of the grouping reactions.", "contents": "Comparison of three methods for grouping streptococci. Two new methods for serological grouping of beta-hemolytic streptococci, the nitrous acid extraction procedure of El Kholy et al. and the slide agglutination method of Christensen et al., were compared with the Lancefield hot-hydrochloric acid extraction method in classifying 92 strains of groups A, B, C, and G. The nitrous acid extraction method was easily performed, specific, and sensitive when highly potent antisera were used. For the Christensen method these highly potent antisera had to be diluted to avoid cross-reactions between groups A and C and groups B and G, respectively. A few strains, most of them group B, could not be grouped by the latter method. Using these three grouping methods, two sets of commercial sera were compared with the more potent sera supplied by R. C. Lancefield. The low antibody content of these commercial sera, especially anti-group B and G sera, contributed to the inferior results obtained in some of the grouping reactions.", "PMID": 956361}
{"id": "pubmed23n0032_2177", "title": "Evaluation of commercial systems for the identification of clinical yeast isolates.", "content": "The Analytab Products Inc. (API), Micro-Drop (MD), and Uni-Yeast-Tek (UYT) systems for the presumptive identification of common clinical yeast isolates were compared with the oxidation-fermentation (OF) and a conventional procedure. With 229 coded isolates, the identification accuracies were API 94, MD 83, OF 82, and UYT 99%. The API system required the greatest technical ability. The MD materials were prone to malfunction. OF media, if incubated beyond 14 days, gave an accuracy of 87%, but this offered no advantage over the conventional procedure. The UYT system was the easiest to use.", "contents": "Evaluation of commercial systems for the identification of clinical yeast isolates. The Analytab Products Inc. (API), Micro-Drop (MD), and Uni-Yeast-Tek (UYT) systems for the presumptive identification of common clinical yeast isolates were compared with the oxidation-fermentation (OF) and a conventional procedure. With 229 coded isolates, the identification accuracies were API 94, MD 83, OF 82, and UYT 99%. The API system required the greatest technical ability. The MD materials were prone to malfunction. OF media, if incubated beyond 14 days, gave an accuracy of 87%, but this offered no advantage over the conventional procedure. The UYT system was the easiest to use.", "PMID": 956362}
{"id": "pubmed23n0032_2178", "title": "Comparison of human fecal and serum parvo-like viruses.", "content": "Parvovirus-like particles found in the sera of two blood donors had the size and appearance on electron microscopy of a virus (B19) found in the serum of a blood donor by Cossart et al. (1975), and those of a virus found in the feces of a normal subject. Antibody to these viruses was detected by immune electron microscopy and immunoelectro-osmophoresis in the sera of 50 children aged 10 to 15 years. Of these, 36% had antibody to the fecal virus, 36% had antibody to B19, and 54% had antibody to the two other serum viruses. The results of these tests suggest that serologically the three serum viruses were similar to one another, but that the fecal virus was distinct. The two blood donors had nonspecific symptoms at the time of viremia. Both donors had developed immunoglobulin M antibody to the virus when tested 3.5 and 4.5 weeks later, but no viruses were detected in the feces or urine.", "contents": "Comparison of human fecal and serum parvo-like viruses. Parvovirus-like particles found in the sera of two blood donors had the size and appearance on electron microscopy of a virus (B19) found in the serum of a blood donor by Cossart et al. (1975), and those of a virus found in the feces of a normal subject. Antibody to these viruses was detected by immune electron microscopy and immunoelectro-osmophoresis in the sera of 50 children aged 10 to 15 years. Of these, 36% had antibody to the fecal virus, 36% had antibody to B19, and 54% had antibody to the two other serum viruses. The results of these tests suggest that serologically the three serum viruses were similar to one another, but that the fecal virus was distinct. The two blood donors had nonspecific symptoms at the time of viremia. Both donors had developed immunoglobulin M antibody to the virus when tested 3.5 and 4.5 weeks later, but no viruses were detected in the feces or urine.", "PMID": 956363}
{"id": "pubmed23n0032_2179", "title": "Growth of Streptococcus mutans on various selective media.", "content": "The ability of Streptococcus mutans to grow on mitis-salivarius (MS) agar, MC agar, mitis-sucrose-bacitracin (MSB), BCY agar, and MM10 sucrose agar was studied. Batch cultures of S. mutans serotype a demonstrated no growth on MSB agar. Certain serotype d and g strains did not grow on MC agar. The yield for most strains of other serotypes on these selective media was lower compared with that on MS agar. The number of total colony-forming units on BCY and MM10 sucrose agar was similar to the blood agar results. Similar data were obtained when fermenter-grown strains, harvested in the middle or the end of the logarithmic growth phase, were used for inoculation of the various media. Enumeration of S. mutans from plaque samples plated on MC and MSB agar yielded about 75% of the counts obtained on MS or the nonselective medium. When the proportions of S. mutans were expressed as a percentage of the total cultivable flora, the selective media (MC and MSB agar) showed approximately 10% lower values than the MS, BCY, and MM10 sucrose agar.", "contents": "Growth of Streptococcus mutans on various selective media. The ability of Streptococcus mutans to grow on mitis-salivarius (MS) agar, MC agar, mitis-sucrose-bacitracin (MSB), BCY agar, and MM10 sucrose agar was studied. Batch cultures of S. mutans serotype a demonstrated no growth on MSB agar. Certain serotype d and g strains did not grow on MC agar. The yield for most strains of other serotypes on these selective media was lower compared with that on MS agar. The number of total colony-forming units on BCY and MM10 sucrose agar was similar to the blood agar results. Similar data were obtained when fermenter-grown strains, harvested in the middle or the end of the logarithmic growth phase, were used for inoculation of the various media. Enumeration of S. mutans from plaque samples plated on MC and MSB agar yielded about 75% of the counts obtained on MS or the nonselective medium. When the proportions of S. mutans were expressed as a percentage of the total cultivable flora, the selective media (MC and MSB agar) showed approximately 10% lower values than the MS, BCY, and MM10 sucrose agar.", "PMID": 956364}
{"id": "pubmed23n0032_2180", "title": "Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci.", "content": "A total of 98.7% of streptococci, groups A,B,C, and G, isolated from various sources was correctly identified by the co-agglutination technique. The active components in this technique are protein A-containing staphylococci coated with antibodies specific for group A,B,C, and G streptococci. A suspension of streptococci belonging to one of these four groups co-agglutinates with the antibody-sensitized staphylococci specific for this group. The technique is extremely rapid and simple and requires no special equipment. It should therefore be a valuable alternative to other techniques used in the grouping of streptococci and is shown here to be as reliable as the Lancefield technique.", "contents": "Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. A total of 98.7% of streptococci, groups A,B,C, and G, isolated from various sources was correctly identified by the co-agglutination technique. The active components in this technique are protein A-containing staphylococci coated with antibodies specific for group A,B,C, and G streptococci. A suspension of streptococci belonging to one of these four groups co-agglutinates with the antibody-sensitized staphylococci specific for this group. The technique is extremely rapid and simple and requires no special equipment. It should therefore be a valuable alternative to other techniques used in the grouping of streptococci and is shown here to be as reliable as the Lancefield technique.", "PMID": 956365}
{"id": "pubmed23n0032_2181", "title": "Enhancement of bactericidal capacity of alveolar macrophages by human alveolar lining material.", "content": "In vivo studies have shown a major role for the alveolar macrophage in the killing of inhaled bacteria. This contrasted with earlier work which showed a preservation of phagocytic properties but a loss of bactericidal capacity when alveolar macrophages were studied in vitro. Recently, alveolar lining material (ALM) from rats has been shown to enhance the in vitro bactericidal capacity of alveolar macrophages from homologous animals against Staphylococcus aureus. Utilizing a similar system, we have confirmed that rat alveolar macrophages do not kill S. aureus in vitro unless the bacteria have been incubated with rat ALM (R-ALM) before phagocytosis. In addition, human ALM (H-ALM) from 7 of 11 patients assayed showed an enhancement of bactericidal capacity by rat alveolar macrophages which was not significantly different from the results utilizing R-ALM. H-ALM from the other four patients gave results which differed significantly from results with H-ALM from the first seven patients and R-ALM (P less than 0.001). Preliminary results suggest that the factor enhancing the bactericidal capacity of rat alveolar macrophages is present in the lipid fraction of the ALM. Further characterization of the ALM is warranted in an effort to explain the enhancement of the bactericidal capacity of alveolar macrophages by most, but not all, H-ALM tested.", "contents": "Enhancement of bactericidal capacity of alveolar macrophages by human alveolar lining material. In vivo studies have shown a major role for the alveolar macrophage in the killing of inhaled bacteria. This contrasted with earlier work which showed a preservation of phagocytic properties but a loss of bactericidal capacity when alveolar macrophages were studied in vitro. Recently, alveolar lining material (ALM) from rats has been shown to enhance the in vitro bactericidal capacity of alveolar macrophages from homologous animals against Staphylococcus aureus. Utilizing a similar system, we have confirmed that rat alveolar macrophages do not kill S. aureus in vitro unless the bacteria have been incubated with rat ALM (R-ALM) before phagocytosis. In addition, human ALM (H-ALM) from 7 of 11 patients assayed showed an enhancement of bactericidal capacity by rat alveolar macrophages which was not significantly different from the results utilizing R-ALM. H-ALM from the other four patients gave results which differed significantly from results with H-ALM from the first seven patients and R-ALM (P less than 0.001). Preliminary results suggest that the factor enhancing the bactericidal capacity of rat alveolar macrophages is present in the lipid fraction of the ALM. Further characterization of the ALM is warranted in an effort to explain the enhancement of the bactericidal capacity of alveolar macrophages by most, but not all, H-ALM tested.", "PMID": 956366}
{"id": "pubmed23n0032_2182", "title": "The effect of indomethacin and other anti-inflammatory drugs on the renin-angiotensin system.", "content": "The administration of two different doses of indomethacin, 9 and 18 mg/kg, to two different groups of rabbits was followed 6 h later by a significant decrease in plasma renin activity, and these levels were not increased by hemorrhage. The administration of 2 mg/kg of indomethacin did not alter the basal levels of plasma renin activity, but it was effective in diminishing the peripheral increase of renin produced by hemorrhage. Similar effects were obtained in other groups of rabbits treated with 9 mg/kg of meclofenamate or 18 mg or aspirin. The lowering effect of indomethacin on plasma renin activity is not specifically related to hemorrhage because it also prevented the increase in plasma renin activity elicited by 5 mg/kg of furosemide. Further studies showed that indomethacin did not exert any significant effect in vivo on the plasma level of renin substrate or on the generation of angiotensin from normal plasma by exogenous renin. And indomethacin did not interfere with the binding capacity of anti-angiotensin I for angiotensin I in the radioimmunoassay reaction or with the in vitro formation of angiotensin from hog renin-nephrectomized rabbit plasma reaction. The results thus indicate that the lowering effect of indomethacin on plasma renin activity is due to the interference with renal renin release. That this effect may be related to the blockade of prostaglandin synthesis is suggested by the similar effect exhibited by other blockers of prostaglandin synthesis.", "contents": "The effect of indomethacin and other anti-inflammatory drugs on the renin-angiotensin system. The administration of two different doses of indomethacin, 9 and 18 mg/kg, to two different groups of rabbits was followed 6 h later by a significant decrease in plasma renin activity, and these levels were not increased by hemorrhage. The administration of 2 mg/kg of indomethacin did not alter the basal levels of plasma renin activity, but it was effective in diminishing the peripheral increase of renin produced by hemorrhage. Similar effects were obtained in other groups of rabbits treated with 9 mg/kg of meclofenamate or 18 mg or aspirin. The lowering effect of indomethacin on plasma renin activity is not specifically related to hemorrhage because it also prevented the increase in plasma renin activity elicited by 5 mg/kg of furosemide. Further studies showed that indomethacin did not exert any significant effect in vivo on the plasma level of renin substrate or on the generation of angiotensin from normal plasma by exogenous renin. And indomethacin did not interfere with the binding capacity of anti-angiotensin I for angiotensin I in the radioimmunoassay reaction or with the in vitro formation of angiotensin from hog renin-nephrectomized rabbit plasma reaction. The results thus indicate that the lowering effect of indomethacin on plasma renin activity is due to the interference with renal renin release. That this effect may be related to the blockade of prostaglandin synthesis is suggested by the similar effect exhibited by other blockers of prostaglandin synthesis.", "PMID": 956367}
{"id": "pubmed23n0032_2183", "title": "De novo synthesis of purine nucleotides in human peripheral blood leukocytes. Excessive activity of the pathway in hypoxanthine-guanine phosphoribosyltransferase deficiency.", "content": "Human peripheral blood leukocytes were studied for the presence and the regulatory properties of the pathway of de novo synthesis of purine nucleotides. The cells were found to incorporate the labeled precursors formate and glycine into purines. The rate of [14C]-formate incorporation was decreased by several compounds known to inhibit purine synthesis by affecting the activity by glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase, the first committed enzyme in the pathway, either through decreasing the availability of PRPP, a substrate for this enzyme, or through exerting inhibition on this enzyme. PRPP availability in the leukocyte was found to be limiting for purine synthesis. Increased PRPP availability resulting from activation of PRPP synthetase by increasing inorganic phosphate (Pi) concentration caused acceleration of purine synthesis. On the other hand, no clear-cut evidence was obtained for the availability of ribose-5-phosphate in the leukocyte being rate limiting at physiological extracellular Pi concentration for PRPP generation, and thus for purine synthesis. However, the addition of methylene blue, which accelerates the oxidative pentose shunt that produces ribose-5-phosphate, resulted in acceleration of PRPP generation and of purine synthesis only when PRPP synthetase was largely activated at high Pi concentration. These results may be taken to suggest that ribose-5-phosphate availability is indeed not limiting for PRPP generation under physiological conditions. Purine synthesis de novo was accelerated more than 13-fold in the leukocytes of two gouty patients affected with partial deficiency of hypoxanthine-guanine phosphoribosyltransferase, but was normal in the leukocytes of an obligate heterozygote for this enzyme abnormality. The results domonstrate in peripheral human leukocytes the presence of the complete pathway of de novo synthesis of purine nucleotides and the manifestation in these cells of the biochemical consequences of hypoxanthine-guanine phosphoribosyltransferase deficiency, i.e., increased availability of PRPP and acceleration of purine synthesis de novo. The results indicate the usefulness of leukocytes as a model tissue for the study of purine metabolism in man.", "contents": "De novo synthesis of purine nucleotides in human peripheral blood leukocytes. Excessive activity of the pathway in hypoxanthine-guanine phosphoribosyltransferase deficiency. Human peripheral blood leukocytes were studied for the presence and the regulatory properties of the pathway of de novo synthesis of purine nucleotides. The cells were found to incorporate the labeled precursors formate and glycine into purines. The rate of [14C]-formate incorporation was decreased by several compounds known to inhibit purine synthesis by affecting the activity by glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase, the first committed enzyme in the pathway, either through decreasing the availability of PRPP, a substrate for this enzyme, or through exerting inhibition on this enzyme. PRPP availability in the leukocyte was found to be limiting for purine synthesis. Increased PRPP availability resulting from activation of PRPP synthetase by increasing inorganic phosphate (Pi) concentration caused acceleration of purine synthesis. On the other hand, no clear-cut evidence was obtained for the availability of ribose-5-phosphate in the leukocyte being rate limiting at physiological extracellular Pi concentration for PRPP generation, and thus for purine synthesis. However, the addition of methylene blue, which accelerates the oxidative pentose shunt that produces ribose-5-phosphate, resulted in acceleration of PRPP generation and of purine synthesis only when PRPP synthetase was largely activated at high Pi concentration. These results may be taken to suggest that ribose-5-phosphate availability is indeed not limiting for PRPP generation under physiological conditions. Purine synthesis de novo was accelerated more than 13-fold in the leukocytes of two gouty patients affected with partial deficiency of hypoxanthine-guanine phosphoribosyltransferase, but was normal in the leukocytes of an obligate heterozygote for this enzyme abnormality. The results domonstrate in peripheral human leukocytes the presence of the complete pathway of de novo synthesis of purine nucleotides and the manifestation in these cells of the biochemical consequences of hypoxanthine-guanine phosphoribosyltransferase deficiency, i.e., increased availability of PRPP and acceleration of purine synthesis de novo. The results indicate the usefulness of leukocytes as a model tissue for the study of purine metabolism in man.", "PMID": 956368}
{"id": "pubmed23n0032_2184", "title": "A micropuncture study of the effect of parathyroid hormone on renal bicarbonate reabsorption.", "content": "Renal micropuncture and clearance experiments were carried out in rats to study the effect of parathyroid hormone (PTH) on renal tubular HCO-/3 reabsorption. The rats were studied during an initial period of parathyroid deficiency (acute thyroidparathyroidectomy, TPTX) and during infusion of large amounts of bovine PTH. Under normal acid-base conditions, PTH administration to TPTX rats caused a significant rise in proximal tubular fluid HCO-/3 concentration (TFHCO-/3), a decrease in fluid reabsorption, and a fall in proximal HCO-/3 reabsorption from 94.0 to 88.2% (P less than 0.01). In control experiments with mannitol infusion, a comparable reduction in proximal fluid reabsorption occurred without any significant effect on intraluminal HCO-/3 concentration. During acute intravenous HCO-/3 loading, PTH inhibited proximal HCO-/3 reabsorption. However, no change in whole kidney HCO-/3 reabsorption was observed in these experiments or in the animals studied under normal acid-base conditions. The findings are consistent with the view that PTH inhibits proximal tubular HCO-/3 reabsorption with normal or high filtered loads of HCO-/3, but distal segments of the nephron are able to reabsorb the excess delivered from the proximal tubule. Measurements of urinary ammonium and titratable acid indicate that net acid excretion (NH+/4 + TA -- HCO-/3) increases significantly after PTH administration. These results do not provide support for the view that PTH excess causes metabolic acidosis by reducing renal acid excretion.", "contents": "A micropuncture study of the effect of parathyroid hormone on renal bicarbonate reabsorption. Renal micropuncture and clearance experiments were carried out in rats to study the effect of parathyroid hormone (PTH) on renal tubular HCO-/3 reabsorption. The rats were studied during an initial period of parathyroid deficiency (acute thyroidparathyroidectomy, TPTX) and during infusion of large amounts of bovine PTH. Under normal acid-base conditions, PTH administration to TPTX rats caused a significant rise in proximal tubular fluid HCO-/3 concentration (TFHCO-/3), a decrease in fluid reabsorption, and a fall in proximal HCO-/3 reabsorption from 94.0 to 88.2% (P less than 0.01). In control experiments with mannitol infusion, a comparable reduction in proximal fluid reabsorption occurred without any significant effect on intraluminal HCO-/3 concentration. During acute intravenous HCO-/3 loading, PTH inhibited proximal HCO-/3 reabsorption. However, no change in whole kidney HCO-/3 reabsorption was observed in these experiments or in the animals studied under normal acid-base conditions. The findings are consistent with the view that PTH inhibits proximal tubular HCO-/3 reabsorption with normal or high filtered loads of HCO-/3, but distal segments of the nephron are able to reabsorb the excess delivered from the proximal tubule. Measurements of urinary ammonium and titratable acid indicate that net acid excretion (NH+/4 + TA -- HCO-/3) increases significantly after PTH administration. These results do not provide support for the view that PTH excess causes metabolic acidosis by reducing renal acid excretion.", "PMID": 956369}
{"id": "pubmed23n0032_2185", "title": "Characteristics of stimulation of H+ transport by aldosterone in turtle urinary bladder.", "content": "Aldosterone stimulates not only Na+ absorption but also urinary acidification. In this investigation the effects of aldosterone on H+ transport are examined in vitro in turtle bladder, a urinary membrane in which several of the factors controlling H+ transport have been defined. H+ transport was increased in bladder halves  exposed to aldosterone compared to control halves. Stimulation of H+ secretion was observed as early as 1 h after addition of aldosterone and occurred before that of Na+ transport. In bladders depleted of endogenous substrate addition of glucose increased H+ transport more in aldosterone-treated halves (10.0+/-1.3 nmol/min) than in control halves (6.8+/-2.3). Addition of pyruvate failed to increase H+ transport (--0.3+/-0.7) in control halves but caused significant increments (2.4+/-0.5) in aldosterone-treated halves. In aldosterone-treated bladders glucose caused larger increments (16.5+/-2.7) in H+ transport than pyruvate (9.3+/-2.0) when halves of the same bladders were compared. Na+ transport, however, was equally increased by the two substrates. Despite the differences in time course and substrate requirements between the stimulation of H+ and Na+ transport, both increases were abolished by actinomycin-D. To examine the effect of aldosterone on the force of the H+ pump, protonmotive force, the pH gradient that would nullify the transport rate was determined with and without aldosterone. Aldosterone did not alter protonmotive force but significantly increased the slope of the H+ transport rate on the applied pH gradient. It is concluded that aldosterone stimulates H+ transport independently of Na+ transport. It increases the responsiveness of the transport rate to glucose and to a lesser extent pyruvate, an effect probably secondary to the increased transport rate. Equivalent circuit analysis indicates that aldosterone facilitates the flow of protons through the active transport pathway but does not increase the force of the pump.", "contents": "Characteristics of stimulation of H+ transport by aldosterone in turtle urinary bladder. Aldosterone stimulates not only Na+ absorption but also urinary acidification. In this investigation the effects of aldosterone on H+ transport are examined in vitro in turtle bladder, a urinary membrane in which several of the factors controlling H+ transport have been defined. H+ transport was increased in bladder halves  exposed to aldosterone compared to control halves. Stimulation of H+ secretion was observed as early as 1 h after addition of aldosterone and occurred before that of Na+ transport. In bladders depleted of endogenous substrate addition of glucose increased H+ transport more in aldosterone-treated halves (10.0+/-1.3 nmol/min) than in control halves (6.8+/-2.3). Addition of pyruvate failed to increase H+ transport (--0.3+/-0.7) in control halves but caused significant increments (2.4+/-0.5) in aldosterone-treated halves. In aldosterone-treated bladders glucose caused larger increments (16.5+/-2.7) in H+ transport than pyruvate (9.3+/-2.0) when halves of the same bladders were compared. Na+ transport, however, was equally increased by the two substrates. Despite the differences in time course and substrate requirements between the stimulation of H+ and Na+ transport, both increases were abolished by actinomycin-D. To examine the effect of aldosterone on the force of the H+ pump, protonmotive force, the pH gradient that would nullify the transport rate was determined with and without aldosterone. Aldosterone did not alter protonmotive force but significantly increased the slope of the H+ transport rate on the applied pH gradient. It is concluded that aldosterone stimulates H+ transport independently of Na+ transport. It increases the responsiveness of the transport rate to glucose and to a lesser extent pyruvate, an effect probably secondary to the increased transport rate. Equivalent circuit analysis indicates that aldosterone facilitates the flow of protons through the active transport pathway but does not increase the force of the pump.", "PMID": 956370}
{"id": "pubmed23n0032_2186", "title": "The relative roles of calcium, phosphorus, and parathyroid hormone in glucose- and tolbutamide-mediated insulin release.", "content": "The relative contributions of Ca++, phosphorus, and parathyroid hormone (PTH) on insulin secretion were evaluated in three groups of dogs. Dogs were studied with glucose infusions (group I) or standard intravenous glucose tolerance tests (IVGTT) (group II) before and after the development of diet-induced hypophosphatemia. Mean serum phosphorus levels for both groups fell from 4.1 to 1.1 mg/100 ml. Animals in group I demonstrated a fall in glucose disappearance rates (Kg) from 5.3+/-0.6% min to 3.5+/-0.5% after induction of hypophosphatemia (P less than 0.001). Mean insulin response was significantly greater in the hypophosphatemic animals than in controls in this group. In group II animals, mean insulin areas obtained during the IVGTT increased from 1,426+/-223 to 2,561+/-141 muU/ml/60 min after induction of hypophosphatemia, and were unaffected by Ca++ or PTH administration. Ca++ administration, but not hypophosphatemia or PTH infusion, increased significantly the mean insulin response to tolbutamide. Secondary hyperparathyroidism was induced by dietary manipulation in four dogs (group III). Mean PTH values increased from 71.4+/-2.1 to 3,012+/-372 pg/ml (P less than 0.001). Mean insulin response to an IVGTT was similar to group III animals, but increased from 1,352+/-128 to 1,894+/-360 muU/ml/60 min after the excessive dietary phosphorus was reduced for 3 mo, and plasma phosphorus fell from 3.2+/-0.1 to 2.8+/-0.3 mg/100 ml. PTH values decreased to 647+/-53 pg/ml. The insulin response to tolbutamide was comparable to that in group II animals, but increased significantly after calcium administration. Immunoreactive insulin disappearance rates were unaffected by hypophosphatemia or diet-induced secondary hyperparathyroidism. These data demonstrate that hypophosphatemia is associated with an augmented glucose-stimulated insulin release, without any effect on tolbutamide-stimulated insulin release. Hypercalcemia produces an augmented tolbutamide-stimulated insulin release with no apparent effect on glucose-stimulated insulin release. Finally, PTH does not appear to be an insulin antagonist and has no apparent effect on either glucose- or tolbutamide-stimulated insulin release in animals with dietary-induced secondary hyperparathyroidism.", "contents": "The relative roles of calcium, phosphorus, and parathyroid hormone in glucose- and tolbutamide-mediated insulin release. The relative contributions of Ca++, phosphorus, and parathyroid hormone (PTH) on insulin secretion were evaluated in three groups of dogs. Dogs were studied with glucose infusions (group I) or standard intravenous glucose tolerance tests (IVGTT) (group II) before and after the development of diet-induced hypophosphatemia. Mean serum phosphorus levels for both groups fell from 4.1 to 1.1 mg/100 ml. Animals in group I demonstrated a fall in glucose disappearance rates (Kg) from 5.3+/-0.6% min to 3.5+/-0.5% after induction of hypophosphatemia (P less than 0.001). Mean insulin response was significantly greater in the hypophosphatemic animals than in controls in this group. In group II animals, mean insulin areas obtained during the IVGTT increased from 1,426+/-223 to 2,561+/-141 muU/ml/60 min after induction of hypophosphatemia, and were unaffected by Ca++ or PTH administration. Ca++ administration, but not hypophosphatemia or PTH infusion, increased significantly the mean insulin response to tolbutamide. Secondary hyperparathyroidism was induced by dietary manipulation in four dogs (group III). Mean PTH values increased from 71.4+/-2.1 to 3,012+/-372 pg/ml (P less than 0.001). Mean insulin response to an IVGTT was similar to group III animals, but increased from 1,352+/-128 to 1,894+/-360 muU/ml/60 min after the excessive dietary phosphorus was reduced for 3 mo, and plasma phosphorus fell from 3.2+/-0.1 to 2.8+/-0.3 mg/100 ml. PTH values decreased to 647+/-53 pg/ml. The insulin response to tolbutamide was comparable to that in group II animals, but increased significantly after calcium administration. Immunoreactive insulin disappearance rates were unaffected by hypophosphatemia or diet-induced secondary hyperparathyroidism. These data demonstrate that hypophosphatemia is associated with an augmented glucose-stimulated insulin release, without any effect on tolbutamide-stimulated insulin release. Hypercalcemia produces an augmented tolbutamide-stimulated insulin release with no apparent effect on glucose-stimulated insulin release. Finally, PTH does not appear to be an insulin antagonist and has no apparent effect on either glucose- or tolbutamide-stimulated insulin release in animals with dietary-induced secondary hyperparathyroidism.", "PMID": 956371}
{"id": "pubmed23n0032_2187", "title": "The effects of sodium ricinoleate on small intestinal function and structure.", "content": "The mechanism of hydroxy fatty acid-induced secretion was investigated in perfused hamster small intestine in vivo. Sodium ricinoleate at an 8-mM concentration resulted in not only secretion of water and sodium, but an increase in intestinal clearance of inulin and a 16,000 mol wt dextran as well. A concentration of ricinoleate (2 mM) which did not affect water transport, however, did not alter intestinal permeability. Ricinoleate-induced intestinal secretion was also accompanied by increased mucosal cell exfoliation as measured by the appearance of DNA in the perfusate and by apparent injury to epithelial cell membranes as judged by measurement of sucrase activity and phospholipid in cell-free aliquots of luminal fluid. Light and electron microscopic studies demonstrated substantial mucosal architectural changes with 8 mM ricinoleate with villus shortening and injury to epithelial cells at the villus tips. In contrast, cholera enterotoxin caused marked secretion of sodium and water, presumably by a cyclic AMP mechanism, but did not alter inulin clearance or enhance DNA or sucrase appearance in the lumen. These studies suggest that at least a component of ricinoleate-induced intestinal secretion is related to structural alterations of the mucosa.", "contents": "The effects of sodium ricinoleate on small intestinal function and structure. The mechanism of hydroxy fatty acid-induced secretion was investigated in perfused hamster small intestine in vivo. Sodium ricinoleate at an 8-mM concentration resulted in not only secretion of water and sodium, but an increase in intestinal clearance of inulin and a 16,000 mol wt dextran as well. A concentration of ricinoleate (2 mM) which did not affect water transport, however, did not alter intestinal permeability. Ricinoleate-induced intestinal secretion was also accompanied by increased mucosal cell exfoliation as measured by the appearance of DNA in the perfusate and by apparent injury to epithelial cell membranes as judged by measurement of sucrase activity and phospholipid in cell-free aliquots of luminal fluid. Light and electron microscopic studies demonstrated substantial mucosal architectural changes with 8 mM ricinoleate with villus shortening and injury to epithelial cells at the villus tips. In contrast, cholera enterotoxin caused marked secretion of sodium and water, presumably by a cyclic AMP mechanism, but did not alter inulin clearance or enhance DNA or sucrase appearance in the lumen. These studies suggest that at least a component of ricinoleate-induced intestinal secretion is related to structural alterations of the mucosa.", "PMID": 956372}
{"id": "pubmed23n0032_2188", "title": "Effects of antihistamines on the lung vascular response to histamine in unanesthetized sheep. Diphenhydramine prevention of pulmonary edema and increased permeability.", "content": "To see whether antihistamines could prevent and reverse histamine-induced pulmonary edema and increased lung vascular permeability, we compared the effects of a 4-h intravenous infusion of 4 mug/kg per min histamine phosphate on pulmonary hemodynamics, lung lymph flow, lymph and plasma protein content, arterial blood gases, hematocrit, and lung water with the effects of an identical histamine infusion given during an infusion of diphenhydramine or metiamide on the same variables in unanesthetized sheep. Histamine caused lymph flow to increase from 6.0+/-0.5 to 27.0+/-5.5 (SEM) ml/h (P less than 0.05), lymph; plasma globulin concentration ratio to increase from 0.62+/-0.01 to 0.67+/-0.02 (P less than 0.05), left atrial pressure to fall from 1+/-1 to -3+/-1 cm H2O (P less than 0.05), and lung lymph clearance of eight protein fractions ranging from 36 to 96 A molecular radius to increase significantly. Histamine also caused increases in lung water, pulmonary vascular resistance, arterial PCO2, pH, and hematocrit, and decreases in cardiac output and arterial PO2. Diphenhydramine (3 mg/kg before histamine followed by 1.5 mg/kg per h intravenous infusion) completely prevented the histamine effect on hematocrit, lung lymph flow, lymph protein clearance, and lung water content, and reduced histamine effects on arterial blood gases and pH. 6 mg/kg diphenhydramine given at the peak histamine response caused lymph flow and lymph: plasma protein concentration ratios to fall. Metiamide (10 mg/kg per h) did not affect the histamine lymph response. We conclude that diphenhydramine can prevent histamine-induced pulmonary edema and can prevent and reverse increased lung vascular permeability caused by histamine, and that histamine effects on lung vascular permeability are H1 actions.", "contents": "Effects of antihistamines on the lung vascular response to histamine in unanesthetized sheep. Diphenhydramine prevention of pulmonary edema and increased permeability. To see whether antihistamines could prevent and reverse histamine-induced pulmonary edema and increased lung vascular permeability, we compared the effects of a 4-h intravenous infusion of 4 mug/kg per min histamine phosphate on pulmonary hemodynamics, lung lymph flow, lymph and plasma protein content, arterial blood gases, hematocrit, and lung water with the effects of an identical histamine infusion given during an infusion of diphenhydramine or metiamide on the same variables in unanesthetized sheep. Histamine caused lymph flow to increase from 6.0+/-0.5 to 27.0+/-5.5 (SEM) ml/h (P less than 0.05), lymph; plasma globulin concentration ratio to increase from 0.62+/-0.01 to 0.67+/-0.02 (P less than 0.05), left atrial pressure to fall from 1+/-1 to -3+/-1 cm H2O (P less than 0.05), and lung lymph clearance of eight protein fractions ranging from 36 to 96 A molecular radius to increase significantly. Histamine also caused increases in lung water, pulmonary vascular resistance, arterial PCO2, pH, and hematocrit, and decreases in cardiac output and arterial PO2. Diphenhydramine (3 mg/kg before histamine followed by 1.5 mg/kg per h intravenous infusion) completely prevented the histamine effect on hematocrit, lung lymph flow, lymph protein clearance, and lung water content, and reduced histamine effects on arterial blood gases and pH. 6 mg/kg diphenhydramine given at the peak histamine response caused lymph flow and lymph: plasma protein concentration ratios to fall. Metiamide (10 mg/kg per h) did not affect the histamine lymph response. We conclude that diphenhydramine can prevent histamine-induced pulmonary edema and can prevent and reverse increased lung vascular permeability caused by histamine, and that histamine effects on lung vascular permeability are H1 actions.", "PMID": 956373}
{"id": "pubmed23n0032_2189", "title": "A role for adenosine deaminase in human monocyte maturation.", "content": "The occurrence of a deficiency of adenosine deaminase (ADA) activity in some patients with severe combined immunodeficiency suggests a possible relationship between the activity of ADA and the aberration of the immune system. To help delineate the function of ADA in the immune response we have examined its role in monocyte maturation. When incubated in vitro, peripheral blood monocytes transformed, within 3 days, to macrophagea as assessed by phase-contrast microscopy and an increase in the specific activity of the lysosomal enzyme acid phosphatase. The specific activity of ADA increased as much as ninefold, reaching a peak after the 1st day in culture, while the activities of other enzymes involved in the purine salvage pathway were not altered. Sucrose density ultracentrifugation of extracts prepared immediately after the isolation of monocytes revealed the presence of two forms of ADA with molecular weights of approximately 30,000 and 110,000. The increase in ADA specific activity during monocyte cultivation correlated with an increase in the activity of the smaller molecular species. A specific inhibitor ADA, erythro-9-(2-hydroxy-3-nonyl) adenine, prevented the increase in acid phosphatase activity, as well as the morphological changes associated with the monocyte maturation. These data suggest a role for ADA in monocyte to macrophage maturation. In view of the central role of macrophages in immune function, this observation may relate to the association of combined immunodeficiency and a deficiency of this enzyme.", "contents": "A role for adenosine deaminase in human monocyte maturation. The occurrence of a deficiency of adenosine deaminase (ADA) activity in some patients with severe combined immunodeficiency suggests a possible relationship between the activity of ADA and the aberration of the immune system. To help delineate the function of ADA in the immune response we have examined its role in monocyte maturation. When incubated in vitro, peripheral blood monocytes transformed, within 3 days, to macrophagea as assessed by phase-contrast microscopy and an increase in the specific activity of the lysosomal enzyme acid phosphatase. The specific activity of ADA increased as much as ninefold, reaching a peak after the 1st day in culture, while the activities of other enzymes involved in the purine salvage pathway were not altered. Sucrose density ultracentrifugation of extracts prepared immediately after the isolation of monocytes revealed the presence of two forms of ADA with molecular weights of approximately 30,000 and 110,000. The increase in ADA specific activity during monocyte cultivation correlated with an increase in the activity of the smaller molecular species. A specific inhibitor ADA, erythro-9-(2-hydroxy-3-nonyl) adenine, prevented the increase in acid phosphatase activity, as well as the morphological changes associated with the monocyte maturation. These data suggest a role for ADA in monocyte to macrophage maturation. In view of the central role of macrophages in immune function, this observation may relate to the association of combined immunodeficiency and a deficiency of this enzyme.", "PMID": 956374}
{"id": "pubmed23n0032_2190", "title": "Free fatty acid oxidation by forearm muscle at rest, and evidence for an intramuscular lipid pool in the human forearm.", "content": "The objects of these experiments were to determine to what extent oleic acid, removed from plasma by forearm muscles, was oxidized immediately, and to search for evidence of an intramuscular lipid pool which may be composed to triglycerides synthesized from plasma free fatty acids and which may supply substantial portions of lipid substrates for oxidation by muscle. To these ends (1-14C]oleic acid was infused at constant rate into the brachial artery of seven healthy young men at rest in the postabsorptive state. Results were: (1) muscle respiratory quotient (0.76) implied that about 80% of the oxygen consumed was for the oxidation of lipid. (2) Muscle free fatty acid uptake, had it been oxidized directly, could account for more than 100% of observed oxygen uptake. (3) There was a lag of at least 30 min before 14CO2 appeared in forearm venous blood. (4) 14CO2 release from forearm muscle tended to reach an apparent plateau after 3 h of infusion of [14C]oleic acid. (5) During the time of plateau 14CO2 release, oleic acid extracted from plasma could account for only 20% of oxygen consumption; most of the oleic acid taken up was not oxidized directly. (6) 14CO2 release persisted at a high level during the 1-3 h follow-up period after the infusion ended. (7) Neither the delay in initial appearance of 14CO2 nor its continued release after cessation of infusion was due to delay and distribution in a forearm CO2 pool, since intra-arterial infusion of NaH14CO3 in additional subjects demonstrated much more rapid distribution of 14CO2 in the forearm. Results show that most, if not all, free fatty acids taken up by resting muscle are not oxidized directly, but probably enter an intramuscular pool which has a slow turnover during resting metabolism and is the immediate source of oxidized lipid substrate.", "contents": "Free fatty acid oxidation by forearm muscle at rest, and evidence for an intramuscular lipid pool in the human forearm. The objects of these experiments were to determine to what extent oleic acid, removed from plasma by forearm muscles, was oxidized immediately, and to search for evidence of an intramuscular lipid pool which may be composed to triglycerides synthesized from plasma free fatty acids and which may supply substantial portions of lipid substrates for oxidation by muscle. To these ends (1-14C]oleic acid was infused at constant rate into the brachial artery of seven healthy young men at rest in the postabsorptive state. Results were: (1) muscle respiratory quotient (0.76) implied that about 80% of the oxygen consumed was for the oxidation of lipid. (2) Muscle free fatty acid uptake, had it been oxidized directly, could account for more than 100% of observed oxygen uptake. (3) There was a lag of at least 30 min before 14CO2 appeared in forearm venous blood. (4) 14CO2 release from forearm muscle tended to reach an apparent plateau after 3 h of infusion of [14C]oleic acid. (5) During the time of plateau 14CO2 release, oleic acid extracted from plasma could account for only 20% of oxygen consumption; most of the oleic acid taken up was not oxidized directly. (6) 14CO2 release persisted at a high level during the 1-3 h follow-up period after the infusion ended. (7) Neither the delay in initial appearance of 14CO2 nor its continued release after cessation of infusion was due to delay and distribution in a forearm CO2 pool, since intra-arterial infusion of NaH14CO3 in additional subjects demonstrated much more rapid distribution of 14CO2 in the forearm. Results show that most, if not all, free fatty acids taken up by resting muscle are not oxidized directly, but probably enter an intramuscular pool which has a slow turnover during resting metabolism and is the immediate source of oxidized lipid substrate.", "PMID": 956375}
{"id": "pubmed23n0032_2191", "title": "Heterogeneity of antibody response to human platelet transfusion.", "content": "To study the antibody response to human platelet transfusions, nine thrombocytopenia patients with bone marrow failure were given 6 U (3X10(11)) of random platelet concentrates twice a week. Before transfusion, none of the patients had preexisting antibodies detectable with lymphocytotoxicity, platelet aggregation, or capillary leukoagglutination techniques. After receiving 18-78 U of platelets, they became refractory to further transfusions of random platelets and alloantibodies were detectable. Two patterns of antibody response could be identified. In three patients, the sera were not lymphocytotoxic with a panel of standard cells in which all the known HLA antigens in the first and second series were represented at least once. Yet, they caused platelet aggregation with 30, 24, and 60%, respectively, of a donor population studied. The aggregating activities were inhibited by antihuman IgG but not by antihuman IgA or antihuman IgM antiserum. The aggregating antibodies could be absorbed out with donor platelets but not lymphocytes or granulocytes. Antibodies from two of these patients aggregated platelets of their respective siblings matched for both HLA haplotypes. Transfusion of platelets from these two siblings did not increase  the platelet count while platelets obtained from aggregation-negative donors did. The sera from the remaining six patients were lymphocytotoxic with 15-100% of the panel of standard cells. They also had aggregating antibodies, which could be absorbed out by both platelets and lymphocytes, suggesting that they were HLA antibodies. These data suggest that the development of platelet-specific antibodies may play an important role in the immunological rejection of isologous platelets, and should be considered in the selection of donors for patients who are refractory to platelets from random donors.", "contents": "Heterogeneity of antibody response to human platelet transfusion. To study the antibody response to human platelet transfusions, nine thrombocytopenia patients with bone marrow failure were given 6 U (3X10(11)) of random platelet concentrates twice a week. Before transfusion, none of the patients had preexisting antibodies detectable with lymphocytotoxicity, platelet aggregation, or capillary leukoagglutination techniques. After receiving 18-78 U of platelets, they became refractory to further transfusions of random platelets and alloantibodies were detectable. Two patterns of antibody response could be identified. In three patients, the sera were not lymphocytotoxic with a panel of standard cells in which all the known HLA antigens in the first and second series were represented at least once. Yet, they caused platelet aggregation with 30, 24, and 60%, respectively, of a donor population studied. The aggregating activities were inhibited by antihuman IgG but not by antihuman IgA or antihuman IgM antiserum. The aggregating antibodies could be absorbed out with donor platelets but not lymphocytes or granulocytes. Antibodies from two of these patients aggregated platelets of their respective siblings matched for both HLA haplotypes. Transfusion of platelets from these two siblings did not increase  the platelet count while platelets obtained from aggregation-negative donors did. The sera from the remaining six patients were lymphocytotoxic with 15-100% of the panel of standard cells. They also had aggregating antibodies, which could be absorbed out by both platelets and lymphocytes, suggesting that they were HLA antibodies. These data suggest that the development of platelet-specific antibodies may play an important role in the immunological rejection of isologous platelets, and should be considered in the selection of donors for patients who are refractory to platelets from random donors.", "PMID": 956376}
{"id": "pubmed23n0032_2192", "title": "Localization of cardiac myosin-specific antibody in myocardial infarction.", "content": "Specific localization of purified antibody against cardiac myosin has been demonstrated in areas of altered myocardial membrane permeability after experimental myocardial infarction. Intravenously administered radioiodine-labeled antimyosin was selectively localized in infarcted myocardium of seven dogs 24 h after coronary occlusion. The mean ratio (+/-SE) of antimyosin antibody in infarcted to normal myocardium in the center of the infarct was 4.2+/-0.4 for endocardial and 2.9+/-0.3 for epicardial layers. By utilizing (Fab')2 fragments of antimyosin obtained by pepsin digestion of purified antibody, the ratio of uptake was increased in eight dogs to 6.1+/-0.6 in the endocardial and 3.3+/-0.4 in the epicardial layers at the infarct center 24 h after occlusion. These ratios were further increased in the infarct center to 13.8+/-1.2 in the endocardial and 7.3+/-0.8 in the epicardial layers when eight dogs were sacrificed 72 h after coronary occlusion. The specificity of antimyosin (Fab')2 localization in infarcted myocardium was demonstrated in four dogs by simultaneous intravenous administration of 125I-labeled antimyosin (Fab')2 and 131I-labeled normal rabbit gamma globulin (Fab')2. Nonspecific trapping of normal rabbit IgG (Fab')2 was observed to be about 38% of total antimyosin (Fab')2 uptake in the central zone of infarction. Regional blood flow was related to antimyosin (Fab')2 uptake in infarcted myocardium by utilizing simultaneous administration of 85Sr-labeled microspheres. An inverse exponential relationship between antimyosin (Fab')2 uptake and regional blood flow was observed (r=0.85). The specific localization of antimyosin antibody or its (Fab')2 components in infarcted myocardium suggests a conceptually new approach to myocardial infarct localization and sizing.", "contents": "Localization of cardiac myosin-specific antibody in myocardial infarction. Specific localization of purified antibody against cardiac myosin has been demonstrated in areas of altered myocardial membrane permeability after experimental myocardial infarction. Intravenously administered radioiodine-labeled antimyosin was selectively localized in infarcted myocardium of seven dogs 24 h after coronary occlusion. The mean ratio (+/-SE) of antimyosin antibody in infarcted to normal myocardium in the center of the infarct was 4.2+/-0.4 for endocardial and 2.9+/-0.3 for epicardial layers. By utilizing (Fab')2 fragments of antimyosin obtained by pepsin digestion of purified antibody, the ratio of uptake was increased in eight dogs to 6.1+/-0.6 in the endocardial and 3.3+/-0.4 in the epicardial layers at the infarct center 24 h after occlusion. These ratios were further increased in the infarct center to 13.8+/-1.2 in the endocardial and 7.3+/-0.8 in the epicardial layers when eight dogs were sacrificed 72 h after coronary occlusion. The specificity of antimyosin (Fab')2 localization in infarcted myocardium was demonstrated in four dogs by simultaneous intravenous administration of 125I-labeled antimyosin (Fab')2 and 131I-labeled normal rabbit gamma globulin (Fab')2. Nonspecific trapping of normal rabbit IgG (Fab')2 was observed to be about 38% of total antimyosin (Fab')2 uptake in the central zone of infarction. Regional blood flow was related to antimyosin (Fab')2 uptake in infarcted myocardium by utilizing simultaneous administration of 85Sr-labeled microspheres. An inverse exponential relationship between antimyosin (Fab')2 uptake and regional blood flow was observed (r=0.85). The specific localization of antimyosin antibody or its (Fab')2 components in infarcted myocardium suggests a conceptually new approach to myocardial infarct localization and sizing.", "PMID": 956377}
{"id": "pubmed23n0032_2193", "title": "Iron deficiency in the rat. Physiological and biochemical studies of muscle dysfunction.", "content": "Work performance on a treadmill has been evaluated in normal and iron-deficient rats. Anemia was removed as a variable by adjusting the hemoglobin of all animals to the same concentration. At a hemoglobin compatible with normal work performance, iron-deficient animals showed a marked impairment of running ability as compared to control animals. Iron therapy corrected the disability within 4 days. Concentrations of the cytochrome pigments and myoglobin, and rates of oxidative phosphorylation with pyruvate-malate, succinate, and alpha-glycerophosphate as substrates were all reduced in mitochondrial preparations from skeletal muscle of iron-deficient rats, but only the rate of phosphorylation with alpha-glycerophosphate as substrate increased significantly and in parallel with the recovery in work performance of the iron-deficient rats treated with iron dextran.", "contents": "Iron deficiency in the rat. Physiological and biochemical studies of muscle dysfunction. Work performance on a treadmill has been evaluated in normal and iron-deficient rats. Anemia was removed as a variable by adjusting the hemoglobin of all animals to the same concentration. At a hemoglobin compatible with normal work performance, iron-deficient animals showed a marked impairment of running ability as compared to control animals. Iron therapy corrected the disability within 4 days. Concentrations of the cytochrome pigments and myoglobin, and rates of oxidative phosphorylation with pyruvate-malate, succinate, and alpha-glycerophosphate as substrates were all reduced in mitochondrial preparations from skeletal muscle of iron-deficient rats, but only the rate of phosphorylation with alpha-glycerophosphate as substrate increased significantly and in parallel with the recovery in work performance of the iron-deficient rats treated with iron dextran.", "PMID": 956378}
{"id": "pubmed23n0032_2194", "title": "The relationship between right duct lymph flow and extravascular lung water in dogs given alpha-naphthylthiourea.", "content": "The relationship between right duct lymph flow and extravascular lung water was studied in 3 normal dogs and 15 dogs with pulmonary edema induced by alpha-naphthylthiourea (ANTU). Right duct lymph was collected in a pouch created by ligating jugular, subclavian, and brachiocephalic veins. Extravascular lung water was measured in vivo by double indicator dilution and post-mortem by weighting lungs before and after drying. Cardiac output, pulmonary artery and pulmonary artery wedge pressures, and the concentration of protein and electrolytes in plasma and right duct lymph were determined. Eight lungs were examined by light and electron microscopy. There was a direct relationship between right duct lymph flow (RDLF in milliters per hour per gram dry lung) and extravascular lung water (Qwl in milliliters per gram dry lung) which was best described by the equation RDLF=0.75-0.26 Qwl+0.03 (Qwl).2 Dogs with severe ANTU-induced edema had extensive lung capillary endothelial destruction but only mild interstitial swelling and no visible damage to type I alveolar epithelial cells. Cardiac output, pulmonary artery and wedge pressures, and protein and electrolyte concentrations did not correlate with either extravascular water or right duct flow. Thus, in ANTU-induced pulmonary edema right duct lymph flow was directly related to extravascular lung water with the highest flows occurring with severe edema. The absence of a rapid increase in lymph flow with small increases in extravascular water may be due to early sequestration of fluid in the alveolar space. Hemodynamic changes did not account for changes in lung water or lymph flow. The pulmonary interstitial factors relating increased extravascular water to lymph drainage remain to be determined.", "contents": "The relationship between right duct lymph flow and extravascular lung water in dogs given alpha-naphthylthiourea. The relationship between right duct lymph flow and extravascular lung water was studied in 3 normal dogs and 15 dogs with pulmonary edema induced by alpha-naphthylthiourea (ANTU). Right duct lymph was collected in a pouch created by ligating jugular, subclavian, and brachiocephalic veins. Extravascular lung water was measured in vivo by double indicator dilution and post-mortem by weighting lungs before and after drying. Cardiac output, pulmonary artery and pulmonary artery wedge pressures, and the concentration of protein and electrolytes in plasma and right duct lymph were determined. Eight lungs were examined by light and electron microscopy. There was a direct relationship between right duct lymph flow (RDLF in milliters per hour per gram dry lung) and extravascular lung water (Qwl in milliliters per gram dry lung) which was best described by the equation RDLF=0.75-0.26 Qwl+0.03 (Qwl).2 Dogs with severe ANTU-induced edema had extensive lung capillary endothelial destruction but only mild interstitial swelling and no visible damage to type I alveolar epithelial cells. Cardiac output, pulmonary artery and wedge pressures, and protein and electrolyte concentrations did not correlate with either extravascular water or right duct flow. Thus, in ANTU-induced pulmonary edema right duct lymph flow was directly related to extravascular lung water with the highest flows occurring with severe edema. The absence of a rapid increase in lymph flow with small increases in extravascular water may be due to early sequestration of fluid in the alveolar space. Hemodynamic changes did not account for changes in lung water or lymph flow. The pulmonary interstitial factors relating increased extravascular water to lymph drainage remain to be determined.", "PMID": 956379}
{"id": "pubmed23n0032_2195", "title": "Regulation of pancreatic and gallbladder functions by intraluminal fatty acids and bile acids in man.", "content": "The effects of intraduodenal glycerol, fatty acid (FA) chain length and FA loads, and bile acid (BA) concentrations on pancreatic and gallbladder function were investigated in 31 healthy volunteers by a perfusion method. FA absorption rates in the duodenum and proximal jejunum were measured simultaneously. Pancreatic and gallbladder responses were augmented by increasing FA chain length and FA loads until the \"maximal\" secretory capacity of the pancreas and gallbladder emptying was attained. Glycerol had no effect. Raising BA concentrations above the critical micellar concentration accelerated FA absorption rates but decreased the magnitude of pancreatic and gallbladder responses to FA. Higher BA concentrations exerted an opposite effect, slowing FA absorption and increasing pancreatic and gallbladder responses. Indeed, a significant, inverse correlation was found between FA absorption and pancreatic and gallbladder responses to FA, suggesting a relationship between the length of intestine exposed to FA and the amount of cholecystokinin (and/or other neurohormonal factors) released, which stimulates pancreatic secretion and gallbladder contraction.", "contents": "Regulation of pancreatic and gallbladder functions by intraluminal fatty acids and bile acids in man. The effects of intraduodenal glycerol, fatty acid (FA) chain length and FA loads, and bile acid (BA) concentrations on pancreatic and gallbladder function were investigated in 31 healthy volunteers by a perfusion method. FA absorption rates in the duodenum and proximal jejunum were measured simultaneously. Pancreatic and gallbladder responses were augmented by increasing FA chain length and FA loads until the \"maximal\" secretory capacity of the pancreas and gallbladder emptying was attained. Glycerol had no effect. Raising BA concentrations above the critical micellar concentration accelerated FA absorption rates but decreased the magnitude of pancreatic and gallbladder responses to FA. Higher BA concentrations exerted an opposite effect, slowing FA absorption and increasing pancreatic and gallbladder responses. Indeed, a significant, inverse correlation was found between FA absorption and pancreatic and gallbladder responses to FA, suggesting a relationship between the length of intestine exposed to FA and the amount of cholecystokinin (and/or other neurohormonal factors) released, which stimulates pancreatic secretion and gallbladder contraction.", "PMID": 956380}
{"id": "pubmed23n0032_2196", "title": "Anion transport processes in the mammalian superficial proximal straight tubule.", "content": "The experiments reported in this paper were designed to evaluate some of the characteristics of anion transport processes during fluid absorption from superficial proximal straight tubules isolated from rabbit kidney. We measured net chemical C1- flux during fluid absorption from tubules perfused and bathed with Krebs-Ringer buffers containing 113.6 mM C1-, 10 mM acetate, and 25 mM HCO-/3 at pH 7.4; assessed the effects of carbonic anhydrase inhibitors on net fluid absorption in the presence and absence of CO2; and evaluated the influx and efflux coefficients for [14C]-acetate transport at 37degreesC, at 21degreesC, and in the presence of carbonic anhydrase inhibitors. The experimental data shown that, for this nephron segment, net C1- flux accompanies approximately 27.5% of net Na+ absorption; and net C1- absorption may be accounted for by a passive transport process, primarily diffusional in nature. Fluid absorption in this nephron segment is reduced 40-60% by carbonic anhydrase inhibitors, but only when the tubules are exposed to 95% O2-5% CO2 rather than 100% O2. Thus, it seems probably that approximately half of Na+ absorption in these tubules may be rationalized in terms of a carbonic anhydrase-dependent CO2 hydration process. In addition, there may occur in these isolated proximal tubules an acetazolamide-insensitive moiety of HCO-/3 absorption comparable to that observed for proximal tubules in vivo. Finally, we provide evidence that net efflux of luminal acetate is due to metabolic energy-dependent processes other than CO2 hydration and may, under appropriate conditions, account for approximately one-fourth of net Na+ absorption.", "contents": "Anion transport processes in the mammalian superficial proximal straight tubule. The experiments reported in this paper were designed to evaluate some of the characteristics of anion transport processes during fluid absorption from superficial proximal straight tubules isolated from rabbit kidney. We measured net chemical C1- flux during fluid absorption from tubules perfused and bathed with Krebs-Ringer buffers containing 113.6 mM C1-, 10 mM acetate, and 25 mM HCO-/3 at pH 7.4; assessed the effects of carbonic anhydrase inhibitors on net fluid absorption in the presence and absence of CO2; and evaluated the influx and efflux coefficients for [14C]-acetate transport at 37degreesC, at 21degreesC, and in the presence of carbonic anhydrase inhibitors. The experimental data shown that, for this nephron segment, net C1- flux accompanies approximately 27.5% of net Na+ absorption; and net C1- absorption may be accounted for by a passive transport process, primarily diffusional in nature. Fluid absorption in this nephron segment is reduced 40-60% by carbonic anhydrase inhibitors, but only when the tubules are exposed to 95% O2-5% CO2 rather than 100% O2. Thus, it seems probably that approximately half of Na+ absorption in these tubules may be rationalized in terms of a carbonic anhydrase-dependent CO2 hydration process. In addition, there may occur in these isolated proximal tubules an acetazolamide-insensitive moiety of HCO-/3 absorption comparable to that observed for proximal tubules in vivo. Finally, we provide evidence that net efflux of luminal acetate is due to metabolic energy-dependent processes other than CO2 hydration and may, under appropriate conditions, account for approximately one-fourth of net Na+ absorption.", "PMID": 956381}
{"id": "pubmed23n0032_2197", "title": "The transport of vitamin D and its 25-hydroxy metabolite in human plasma. Isolation and partial characterization of vitamin D and 25-hydroxyvitamin D binding protein.", "content": "This study reports the isolation and partial characterization of vitamin D and 25-hydroxyvitamin D binding protein (DBP), the specific transport protein for vitamin D and its 25-hydroxy metabolite in human plasma. DBP was labeled by the addition of a tracer amount of 3H-labeled 25-OH-D3 to the original plasma used for protein fractionation. Previous experiments have shown that such 25-OH-D3 added in vitro binds to the same protein normally responsible for the transport of endogenous 25-OH-D and of vitamin D. The isolation of human DBP was achieved by an extensive sequence of procedures which resulted in a final yield of only approximately 4 mg of purified DBP from a starting volume of 34 liters of plasma. Purified DBP was homogeneous in the analytical ultracentrifuge and showed a single band of protein on analytical polyacrylamide gel electrophoresis. DBP had a sedimentation constant of 3.49s and a mol wt of approximately 52,000. The molecular weight was assessed by sedimentation equilibrium analysis and also by sodium dodecyl sulfate-disc-gel electrophoresis and by gel filtration on a standardized column of Sephadex G-150. The amino acid composition of DBP was determined and was generally consistent with the estimated extinction coefficient (E1cm1% at 280 nm) of about 9.1. The isoelectric point of DBP was estimated as 4.8 from isoelectric focusing experiments. Direct study of the binding capacity of the purified DBP for added 25-OH-D3 showed that the isolated DBP had a high affinity for 25-OH-D3, with an apparent maximum binding capacity of one molecule of 25-OH-D3 per molecule of protein.", "contents": "The transport of vitamin D and its 25-hydroxy metabolite in human plasma. Isolation and partial characterization of vitamin D and 25-hydroxyvitamin D binding protein. This study reports the isolation and partial characterization of vitamin D and 25-hydroxyvitamin D binding protein (DBP), the specific transport protein for vitamin D and its 25-hydroxy metabolite in human plasma. DBP was labeled by the addition of a tracer amount of 3H-labeled 25-OH-D3 to the original plasma used for protein fractionation. Previous experiments have shown that such 25-OH-D3 added in vitro binds to the same protein normally responsible for the transport of endogenous 25-OH-D and of vitamin D. The isolation of human DBP was achieved by an extensive sequence of procedures which resulted in a final yield of only approximately 4 mg of purified DBP from a starting volume of 34 liters of plasma. Purified DBP was homogeneous in the analytical ultracentrifuge and showed a single band of protein on analytical polyacrylamide gel electrophoresis. DBP had a sedimentation constant of 3.49s and a mol wt of approximately 52,000. The molecular weight was assessed by sedimentation equilibrium analysis and also by sodium dodecyl sulfate-disc-gel electrophoresis and by gel filtration on a standardized column of Sephadex G-150. The amino acid composition of DBP was determined and was generally consistent with the estimated extinction coefficient (E1cm1% at 280 nm) of about 9.1. The isoelectric point of DBP was estimated as 4.8 from isoelectric focusing experiments. Direct study of the binding capacity of the purified DBP for added 25-OH-D3 showed that the isolated DBP had a high affinity for 25-OH-D3, with an apparent maximum binding capacity of one molecule of 25-OH-D3 per molecule of protein.", "PMID": 956382}
{"id": "pubmed23n0032_2198", "title": "Purification and properties of sulfite oxidase from human liver.", "content": "Sulfite oxidase has been purified to near homogeneity from human liver. Properties of the molecule have been investigated and compared to those of the rat liver enzyme which has been isolated in a pure form. Both proteins exist as dimeric molecules with one molybdenum and one cytochrome b5-type heme per sub-unit. The human enzyme has a slightly larger subunit molecular weight (61,100 vs. 57,200 daltons) and is a more negatively charged molecule. Decreased reactivity of the human enzyme with various electron acceptors suggests the presence of nonfunctional molybdenum centers in a portion of the molecules isolated. Human liver sulfite oxidase cross-reacts strongly with antibody prepared against the rat liver enzyme. Human enzyme activity is precipitated by antibody at concentrations about twofold greater than required for comparable complexation of rat sulfite oxidase.", "contents": "Purification and properties of sulfite oxidase from human liver. Sulfite oxidase has been purified to near homogeneity from human liver. Properties of the molecule have been investigated and compared to those of the rat liver enzyme which has been isolated in a pure form. Both proteins exist as dimeric molecules with one molybdenum and one cytochrome b5-type heme per sub-unit. The human enzyme has a slightly larger subunit molecular weight (61,100 vs. 57,200 daltons) and is a more negatively charged molecule. Decreased reactivity of the human enzyme with various electron acceptors suggests the presence of nonfunctional molybdenum centers in a portion of the molecules isolated. Human liver sulfite oxidase cross-reacts strongly with antibody prepared against the rat liver enzyme. Human enzyme activity is precipitated by antibody at concentrations about twofold greater than required for comparable complexation of rat sulfite oxidase.", "PMID": 956383}
{"id": "pubmed23n0032_2199", "title": "Human sulfite oxidase deficiency. Characterization of the molecular defect in a multicomponent system.", "content": "Frozen liver tissue from an individual identified several years ago as sulfite oxidase deficient has been reexamined in light of new knowledge which has been obtained regarding the enzyme. It has been established that hepatic molybdenum levels and xanthine oxidase activity were within normal values and comparable to those observed in control samples preserved from the original study along with the deficient tissue sample. The ability of the patient's liver to synthesize the specific molybdenum cofactor required for activation of de-molybdo sulfite oxidase also appears to have been unimpaired. Using an antibody preparation directed against rat liver sulfite oxidase which also inhibits and precipitates the human enzyme, it has been determined that cross-reacting material with determinants recognized by inhibiting antibodies is absent in the liver sample from the deficient patient. Immunodiffusion experiments gave strong precipitin bands against the control liver extracts, but showed no detectable precipitin reaction between the deficient liver extract and the antibody preparation. The relationship of these findings to a second patient recently identified as sulfite oxidase deficient and to an animal model of the disease are discussed.", "contents": "Human sulfite oxidase deficiency. Characterization of the molecular defect in a multicomponent system. Frozen liver tissue from an individual identified several years ago as sulfite oxidase deficient has been reexamined in light of new knowledge which has been obtained regarding the enzyme. It has been established that hepatic molybdenum levels and xanthine oxidase activity were within normal values and comparable to those observed in control samples preserved from the original study along with the deficient tissue sample. The ability of the patient's liver to synthesize the specific molybdenum cofactor required for activation of de-molybdo sulfite oxidase also appears to have been unimpaired. Using an antibody preparation directed against rat liver sulfite oxidase which also inhibits and precipitates the human enzyme, it has been determined that cross-reacting material with determinants recognized by inhibiting antibodies is absent in the liver sample from the deficient patient. Immunodiffusion experiments gave strong precipitin bands against the control liver extracts, but showed no detectable precipitin reaction between the deficient liver extract and the antibody preparation. The relationship of these findings to a second patient recently identified as sulfite oxidase deficient and to an animal model of the disease are discussed.", "PMID": 956384}
{"id": "pubmed23n0032_2200", "title": "Stimulation of ammonia production and excretion in the rabbit by inorganic phosphate. Study of control mechanisms.", "content": "The purpose of this study was to clarify the mechanism (s) responsible for regulation of ammonia production and excretion in the rabbit. The normally low ammonia excretion rate during acute metabolic acidosis was stimulated acutely and increased approximately ninefold after infusion of sodium phosphate, but remained low if sodium sulphate or Tris was substituted for phosphate. Ammonia production was increased significantly by phosphate in rabbit renal cortex slices and in isolated renal cortex mitochondria. In isolated mitochondria, mersalyl, an inhibitor of both the phosphate/hydroxyl and phosphate/dicarboxylate mitochondrial carriers, inhibited the phosphate-induced stimulation, indicating that phosphate must enter the mitochondrion for stimulation. A malate/phosphate exchange seemed to be involved since N-ethylmaleimide, an inhibitor of the phosphate/hydroxyl exchange, did not inhibit phosphate-stimulated ammonia production, whereas there was inhibition by 2-n-butylmalonate, a competitive inhibitor of the dicarboxylate carrier. Phosphate itself was not essential since malonate stimulated ammoniagenesis in the absence of added phosphate. Similarly, citrate stimulated ammoniagenesis in isolated mitochondria in the absence of inorganic phosphate provided that it induced L-malate exit on the citrate transporter associated with inhibition of citrate oxidation by fluoroacetate. Similar results were also seen in mitochondria from rat renal cortex. A fall in mitochondrial alpha-ketoglutarate level resulted in an increase in ammonia production. This could be achieved directly with malonate or indirectly via L-malate exit. Simultaneous measurements of glutamate showed that the rate of ammonia production was reciprocally related to the glutamate content. We conclude that phosphate-induced stimulation of ammoniagenesis in the rabbit kidney is mediated by removal of glutamate, the feedback inhibitor of phosphate-dependent glutaminase. Glutamate removal is linked to phosphate-induced dicarboxylate exit across the mitochondrial membrane.", "contents": "Stimulation of ammonia production and excretion in the rabbit by inorganic phosphate. Study of control mechanisms. The purpose of this study was to clarify the mechanism (s) responsible for regulation of ammonia production and excretion in the rabbit. The normally low ammonia excretion rate during acute metabolic acidosis was stimulated acutely and increased approximately ninefold after infusion of sodium phosphate, but remained low if sodium sulphate or Tris was substituted for phosphate. Ammonia production was increased significantly by phosphate in rabbit renal cortex slices and in isolated renal cortex mitochondria. In isolated mitochondria, mersalyl, an inhibitor of both the phosphate/hydroxyl and phosphate/dicarboxylate mitochondrial carriers, inhibited the phosphate-induced stimulation, indicating that phosphate must enter the mitochondrion for stimulation. A malate/phosphate exchange seemed to be involved since N-ethylmaleimide, an inhibitor of the phosphate/hydroxyl exchange, did not inhibit phosphate-stimulated ammonia production, whereas there was inhibition by 2-n-butylmalonate, a competitive inhibitor of the dicarboxylate carrier. Phosphate itself was not essential since malonate stimulated ammoniagenesis in the absence of added phosphate. Similarly, citrate stimulated ammoniagenesis in isolated mitochondria in the absence of inorganic phosphate provided that it induced L-malate exit on the citrate transporter associated with inhibition of citrate oxidation by fluoroacetate. Similar results were also seen in mitochondria from rat renal cortex. A fall in mitochondrial alpha-ketoglutarate level resulted in an increase in ammonia production. This could be achieved directly with malonate or indirectly via L-malate exit. Simultaneous measurements of glutamate showed that the rate of ammonia production was reciprocally related to the glutamate content. We conclude that phosphate-induced stimulation of ammoniagenesis in the rabbit kidney is mediated by removal of glutamate, the feedback inhibitor of phosphate-dependent glutaminase. Glutamate removal is linked to phosphate-induced dicarboxylate exit across the mitochondrial membrane.", "PMID": 956385}
{"id": "pubmed23n0032_2201", "title": "Development of glucagon sensitivity in neonatal rat liver.", "content": "The ontogenesis of the hepatic glucagon-sensitive adenylate cyclase system has been studied in the rat. With a partially purified liver membrane preparation, fetal adenylate cyclase was less responsive to glucagon than the enzyme from neonatal or adult livers. Similar results were obtained in gently prepared liver homogenates, suggesting that destruction of essential components of the fetal liver membrane did not account for the relative unresponsiveness of the adenylate cyclase enzyme to glucagon. Investigation of other factors that might account for diminished fetal hepatic responsiveness to glucagon indicate (a) minimal glucagon degradation by fetal membranes relative to 8-day or adult tissue; and (b) available adenylate cyclase enzyme, as suggested by a 13-fold increase over basal cyclic AMP formation with NaF in fetal liver membranes. These results indicate that neither enhanced glucagon degradation nor adenylate cyclase enzyme deficiency accounts for the relative insensitivity of the fetal hepatic adenylate cyclase system to glucagon. In early neonatal life, hepatic adenylate cyclase responsiveness to glucagon rapidly developed and was maximal 6 days after birth. These changes were closely paralleled by a fivefold increase in glucagon binding and the kinetically determined Vmax for cyclic AMP formation. These observations suggest that (a) fetal hepatic unresponsiveness to glucagon may be explained by a limited number of glucagon receptor sites; (b) during the neonatal period, the development of glucagon binding is expressed primarily as an increase in adenylate cyclase Vmax; (c) the ontogenesis of hepatic responsiveness to glucagon may be important in the resolution of neonatal hypoglycemia.", "contents": "Development of glucagon sensitivity in neonatal rat liver. The ontogenesis of the hepatic glucagon-sensitive adenylate cyclase system has been studied in the rat. With a partially purified liver membrane preparation, fetal adenylate cyclase was less responsive to glucagon than the enzyme from neonatal or adult livers. Similar results were obtained in gently prepared liver homogenates, suggesting that destruction of essential components of the fetal liver membrane did not account for the relative unresponsiveness of the adenylate cyclase enzyme to glucagon. Investigation of other factors that might account for diminished fetal hepatic responsiveness to glucagon indicate (a) minimal glucagon degradation by fetal membranes relative to 8-day or adult tissue; and (b) available adenylate cyclase enzyme, as suggested by a 13-fold increase over basal cyclic AMP formation with NaF in fetal liver membranes. These results indicate that neither enhanced glucagon degradation nor adenylate cyclase enzyme deficiency accounts for the relative insensitivity of the fetal hepatic adenylate cyclase system to glucagon. In early neonatal life, hepatic adenylate cyclase responsiveness to glucagon rapidly developed and was maximal 6 days after birth. These changes were closely paralleled by a fivefold increase in glucagon binding and the kinetically determined Vmax for cyclic AMP formation. These observations suggest that (a) fetal hepatic unresponsiveness to glucagon may be explained by a limited number of glucagon receptor sites; (b) during the neonatal period, the development of glucagon binding is expressed primarily as an increase in adenylate cyclase Vmax; (c) the ontogenesis of hepatic responsiveness to glucagon may be important in the resolution of neonatal hypoglycemia.", "PMID": 956386}
{"id": "pubmed23n0032_2202", "title": "On the influence of the natriuretic factor from patients with chronic uremia on the bioelectric properties and sodium transport of the isolated mammalian collecting tubule.", "content": "A gel filtration fraction of urine from patients with chronic renal disease (natriuretic factor) has been shown previously to cause natriuresis in rats and to inhibit sodium transport in the isolated toad bladder. The effect of this fraction on transtubular potential difference and sodium transport was examined on the isolated perfused cortical collecting tubule of the rabbit. A rapid inhibition of potential difference from -22.5 mV to -12 mV (P less than 0.001) was observed when the fraction was applied to the peritubular surface. This effect was accompanied by a decrease in net sodium flux from 6.29 to 3.21 pmol/cm per s (P less than 0.001). Unidirectional fluxes using isotopic sodium revealed that the inhibition of net sodium transport was due to a decrease in flux from the lumen to the peritubular surface, i.e., an inhibition of active sodium transport. There was no change in sodium flux in the reverse direction. These changes were all rapidly reversed by removal of the fraction from the peritubular surface. The addition of the fraction to the lumen had no effect on potential difference or net sodium flux. Control studies using the same fraction from the urine of normal subjects had no effect on any of the parameters studies. Where both a uremic and a normal fraction were sequentially applied to the peritubular surface of the same tubule, inhibition of potential difference was obtained only with the former. In the light of evidence implicating the collecting duct fraction from normal animals, the data are consistent with the view that the natriuretic factor may be biologically important in the regulation of sodium balance via it's regulatory role in active sodium transport in the collecting tubule.", "contents": "On the influence of the natriuretic factor from patients with chronic uremia on the bioelectric properties and sodium transport of the isolated mammalian collecting tubule. A gel filtration fraction of urine from patients with chronic renal disease (natriuretic factor) has been shown previously to cause natriuresis in rats and to inhibit sodium transport in the isolated toad bladder. The effect of this fraction on transtubular potential difference and sodium transport was examined on the isolated perfused cortical collecting tubule of the rabbit. A rapid inhibition of potential difference from -22.5 mV to -12 mV (P less than 0.001) was observed when the fraction was applied to the peritubular surface. This effect was accompanied by a decrease in net sodium flux from 6.29 to 3.21 pmol/cm per s (P less than 0.001). Unidirectional fluxes using isotopic sodium revealed that the inhibition of net sodium transport was due to a decrease in flux from the lumen to the peritubular surface, i.e., an inhibition of active sodium transport. There was no change in sodium flux in the reverse direction. These changes were all rapidly reversed by removal of the fraction from the peritubular surface. The addition of the fraction to the lumen had no effect on potential difference or net sodium flux. Control studies using the same fraction from the urine of normal subjects had no effect on any of the parameters studies. Where both a uremic and a normal fraction were sequentially applied to the peritubular surface of the same tubule, inhibition of potential difference was obtained only with the former. In the light of evidence implicating the collecting duct fraction from normal animals, the data are consistent with the view that the natriuretic factor may be biologically important in the regulation of sodium balance via it's regulatory role in active sodium transport in the collecting tubule.", "PMID": 956387}
{"id": "pubmed23n0032_2203", "title": "Type II hyperprolinemia. Delta1-pyrroline-5-carboxylic acid dehydrogenase deficiency in cultured skin fibroblasts and circulating lymphocytes.", "content": "Type II hyperprolinemia is an inherited abnormality in amino acid metabolism characterized by elevated plasma proline concentrations, iminoglycinuria, and the urinary excretion of delta1-pyrroline compounds. To define the enzymologic defect of this biochemical disorder, we developed a specific, sensitive radioisotopic assay for the proline degradative enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase. Using this assay, we have shown an absence of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in the cultured fibroblasts from three patients with type II hyperprolinemia. We confirmed this result on cultured cells by demonstrating a similar absence of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in extracts prepared from the peripheral leukocytes of these patients. Additionally, we found significantly decreased levels of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in the leukocyte extracts from five obligate heterozygotes for type II hyperprolinemia. We also demonstrated a reduction in leukocyte delta1-pyrroline-5-carboxylic acid dehydrogenase activity in three successive generations of a family. These results prove that an absence of delta1-pyrroline-5-carboxylic acid dehydrogenase is the enzymologic defect in type II hyperprolinemia and that this defect is inherited in an autosomal recessive fashion.", "contents": "Type II hyperprolinemia. Delta1-pyrroline-5-carboxylic acid dehydrogenase deficiency in cultured skin fibroblasts and circulating lymphocytes. Type II hyperprolinemia is an inherited abnormality in amino acid metabolism characterized by elevated plasma proline concentrations, iminoglycinuria, and the urinary excretion of delta1-pyrroline compounds. To define the enzymologic defect of this biochemical disorder, we developed a specific, sensitive radioisotopic assay for the proline degradative enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase. Using this assay, we have shown an absence of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in the cultured fibroblasts from three patients with type II hyperprolinemia. We confirmed this result on cultured cells by demonstrating a similar absence of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in extracts prepared from the peripheral leukocytes of these patients. Additionally, we found significantly decreased levels of delta1-pyrroline-5-carboxylic acid dehydrogenase activity in the leukocyte extracts from five obligate heterozygotes for type II hyperprolinemia. We also demonstrated a reduction in leukocyte delta1-pyrroline-5-carboxylic acid dehydrogenase activity in three successive generations of a family. These results prove that an absence of delta1-pyrroline-5-carboxylic acid dehydrogenase is the enzymologic defect in type II hyperprolinemia and that this defect is inherited in an autosomal recessive fashion.", "PMID": 956388}
{"id": "pubmed23n0032_2204", "title": "Urea secretion by the straight segment of the proximal tubule.", "content": "Studies utilizing in vitro microperfusion were designed to examine whether urea is actively or passively transported across superficial and juxtamedullary straight segments of rabbit proximal tubules. With perfusate and bath solutions containing 1 mM urea and electrolytes similar to normal plasma, the efflux (lumen-to-bath) isotopic permeability (X 10(-5) cm s-1) of superficial segments was 1.37 +/- 0.16 and of juxtamedullary segments was 2.14 +/- 0.20. In the same tubules, the influx (bath-to-lumen) isotopic permeability was 3.70 +/- 0.35 in superficial segments and 4.75 +/- 0.37 in juxtamedullary segments. Despite net water movement in the opposite direction (0.5 nl mm-1 min-1), the influx rate was significantly higher than the efflux rate of urea in both groups. With a low perfusion rate (2 nl/min) and equivalent specific activities of [14C]urea in bath and perfusate, the collected-to-perfused ratio of [14C]urea, corrected for volume marker change, was 1.07 +/- 0.01 in superficial and 1.09 +/- 0.01 in juxtamedullary nephrons, thus indicating net secretion in both segments. In separate studies urea influx was inhibited by hypothermia (decrease from 37 degrees to 28 degrees C), by phloretin (0.1 mM in bath), by cyanide (1 mM), but not by probenecid (0.2 mM). In each case the inhibition was highly significant and reversible. These data suggest that urea is actively secreted by the straight segments of both the superficial and juxtamedullary proximal tubules. These segments may, therefore, contribute significantly to the high urea concentration found at the bend of Henle's loop by micropuncture.", "contents": "Urea secretion by the straight segment of the proximal tubule. Studies utilizing in vitro microperfusion were designed to examine whether urea is actively or passively transported across superficial and juxtamedullary straight segments of rabbit proximal tubules. With perfusate and bath solutions containing 1 mM urea and electrolytes similar to normal plasma, the efflux (lumen-to-bath) isotopic permeability (X 10(-5) cm s-1) of superficial segments was 1.37 +/- 0.16 and of juxtamedullary segments was 2.14 +/- 0.20. In the same tubules, the influx (bath-to-lumen) isotopic permeability was 3.70 +/- 0.35 in superficial segments and 4.75 +/- 0.37 in juxtamedullary segments. Despite net water movement in the opposite direction (0.5 nl mm-1 min-1), the influx rate was significantly higher than the efflux rate of urea in both groups. With a low perfusion rate (2 nl/min) and equivalent specific activities of [14C]urea in bath and perfusate, the collected-to-perfused ratio of [14C]urea, corrected for volume marker change, was 1.07 +/- 0.01 in superficial and 1.09 +/- 0.01 in juxtamedullary nephrons, thus indicating net secretion in both segments. In separate studies urea influx was inhibited by hypothermia (decrease from 37 degrees to 28 degrees C), by phloretin (0.1 mM in bath), by cyanide (1 mM), but not by probenecid (0.2 mM). In each case the inhibition was highly significant and reversible. These data suggest that urea is actively secreted by the straight segments of both the superficial and juxtamedullary proximal tubules. These segments may, therefore, contribute significantly to the high urea concentration found at the bend of Henle's loop by micropuncture.", "PMID": 956389}
{"id": "pubmed23n0032_2205", "title": "Cytotoxic activity of rheumatoid and normal lymphocytes against allogeneic and autologous synovial cells in vitro.", "content": "The possibility that lymphocytes from patients with rheumatoid arthritis (RA) might be sensitized to RA synovial cell antigens was investigated with a 51Cr release cytotoxicity assay. Peripheral blood lymphocytes from rheumatoid and normal donors were tested for cytotoxic activity against their own synovial cells and against allogeneic rheumatoid and nonrhemuatoid synovial cells. In the allogeneic studies, the degree of cytotoxicity was significantly influenced by the age in culture (passage number) of the synovial target cells (P less than 0.001). When the passage number of the target cells was considered in the analysis, rheumatoid lymphocytes were found to have greater cytotoxic activity than normal lymphocytes against young cultures (low passage number) of both RA and non-RA synovial cells (P = 0.0042). Differences in susceptibility to lysis between RA and non-RA synovial cells were more susceptible to both RA and normal lymphocyte-induced lysis than were non-RA synovial cells (P = 0.0048). No evidence of cytotoxicity was detected when lymphocytes from nine RA patients and two osteoarthritis patients were reacted against their own synovial cells. Although the data demonstrated an increased cytotoxic activity of peripheral blood lymphocytes from some RA patients against allogeneic synovial cells, the fact that this reactivity was seen against both non-RA and RA synovial cells and was not demonstrated against autologous synovial cells argues against the presence of an immunospecific response of RA lymphocytes to RA synovial cell antigens.", "contents": "Cytotoxic activity of rheumatoid and normal lymphocytes against allogeneic and autologous synovial cells in vitro. The possibility that lymphocytes from patients with rheumatoid arthritis (RA) might be sensitized to RA synovial cell antigens was investigated with a 51Cr release cytotoxicity assay. Peripheral blood lymphocytes from rheumatoid and normal donors were tested for cytotoxic activity against their own synovial cells and against allogeneic rheumatoid and nonrhemuatoid synovial cells. In the allogeneic studies, the degree of cytotoxicity was significantly influenced by the age in culture (passage number) of the synovial target cells (P less than 0.001). When the passage number of the target cells was considered in the analysis, rheumatoid lymphocytes were found to have greater cytotoxic activity than normal lymphocytes against young cultures (low passage number) of both RA and non-RA synovial cells (P = 0.0042). Differences in susceptibility to lysis between RA and non-RA synovial cells were more susceptible to both RA and normal lymphocyte-induced lysis than were non-RA synovial cells (P = 0.0048). No evidence of cytotoxicity was detected when lymphocytes from nine RA patients and two osteoarthritis patients were reacted against their own synovial cells. Although the data demonstrated an increased cytotoxic activity of peripheral blood lymphocytes from some RA patients against allogeneic synovial cells, the fact that this reactivity was seen against both non-RA and RA synovial cells and was not demonstrated against autologous synovial cells argues against the presence of an immunospecific response of RA lymphocytes to RA synovial cell antigens.", "PMID": 956390}
{"id": "pubmed23n0032_2206", "title": "Studies on the mechanisms of food-stimulated gastric acid secretion in normal human subjects.", "content": "Liquid test meals were infused into the stomach and acid secretion was measured by intragastric titration at pH 5.0 Acid secretion after 500 or 750-ml sodium chloride meals was two to three times higher than basal secretion rates and was equivalent to 25-30% of the peak acid output in response to histamine. Since these meals did not cause a rise in serum gastrin concentration, it is assumed that they stimulate acid secretion by causing distention of the body and fundus of the stomach. Compared with this distention stimulus, glucose meals had no effect on acid secretion and fat-inhibited acid secretion; however, both glucose and fat caused an increase in serum gastrin concentration. Amino acids caused a much greater increase in serum gastrin concentration and enhanced acid secretion above that noted with distention alone. In contrast, albumin did not enhance the serum gastrin concentration or stimulate acid secretion to a statistically significant extent. There was a close correlation between the rise in serum gastrin concentration and rate of acid secretion after different test meals when average results for each test meal were plotted. However, there was a poor correlation between acid secretion and serum gastrin concentration when the responses of the individual subjects with a given test meal were compared. Our interpretations are: (a) Distention is an important stimulant of the acid-secretory response to a meal, and this is not mediated by gastrin release. (b) Gastrin is one but probably not the only mediator of the chemical phase of acid secretion, i.e., acid secretion noted with amino acids that cannot be explained by distention. (c) Glucose and fat also release gastrin; however, with glucose the rise in serum gastrin is too small and too transient to enhance acid secretion, and fat probably releases unmeasured inhibitors that overwhelm the effect of gastrin on acid secretion. (d) Albumin is not a stimulant of acid secretion.", "contents": "Studies on the mechanisms of food-stimulated gastric acid secretion in normal human subjects. Liquid test meals were infused into the stomach and acid secretion was measured by intragastric titration at pH 5.0 Acid secretion after 500 or 750-ml sodium chloride meals was two to three times higher than basal secretion rates and was equivalent to 25-30% of the peak acid output in response to histamine. Since these meals did not cause a rise in serum gastrin concentration, it is assumed that they stimulate acid secretion by causing distention of the body and fundus of the stomach. Compared with this distention stimulus, glucose meals had no effect on acid secretion and fat-inhibited acid secretion; however, both glucose and fat caused an increase in serum gastrin concentration. Amino acids caused a much greater increase in serum gastrin concentration and enhanced acid secretion above that noted with distention alone. In contrast, albumin did not enhance the serum gastrin concentration or stimulate acid secretion to a statistically significant extent. There was a close correlation between the rise in serum gastrin concentration and rate of acid secretion after different test meals when average results for each test meal were plotted. However, there was a poor correlation between acid secretion and serum gastrin concentration when the responses of the individual subjects with a given test meal were compared. Our interpretations are: (a) Distention is an important stimulant of the acid-secretory response to a meal, and this is not mediated by gastrin release. (b) Gastrin is one but probably not the only mediator of the chemical phase of acid secretion, i.e., acid secretion noted with amino acids that cannot be explained by distention. (c) Glucose and fat also release gastrin; however, with glucose the rise in serum gastrin is too small and too transient to enhance acid secretion, and fat probably releases unmeasured inhibitors that overwhelm the effect of gastrin on acid secretion. (d) Albumin is not a stimulant of acid secretion.", "PMID": 956391}
{"id": "pubmed23n0032_2207", "title": "Hematologic and clinical responses in patients with sickle cell anemia after chronic extracorporeal red cell carbamylation.", "content": "In eight patients with sickle cell anemia, weekly extracorporeal carbamylation of about 20% of the circulating red cell mass was carried out for 2 yr or longer. At each visit, a mean of 1.3+/-0.2 mol of cyanate were incorporated per mole of hemoglobin in the carbamylated erythrocytes. Within 3 mo, a stable level of about 35-50% of the circulating erythrocytes was carbamylated. This quantity and degree of hemoglobin carbamylation produced a decrease in mean whole blood P50 from 33 to 26 mm Hg. During the first 3 mo of carbamylation, the mean hemoglobin increased from 6.4 to 9.1 g/100 ml, while mean absolute reticulocytes decreased by 58% and circulating irreversibly sickled erythrocytes decreased by 65%. The mean red cell life span increased from 13 days before treatment to 21.6 days after 3 mo of carbamylation. Beyond the 3rd mo of carbamylation, blood P50, hemoglobin, and reticulocytes remained quite stable. No toxic effects of extracorporeal carbamylation of erythrocytes were noted. The capacity of blood to release oxygen at 30 mm Hg PO2 increased from 4.3 to 5.0 cm3/100 ml blood during carbamylation. The overall frequency of severe painful crises decreased by about 80% during carbamylation. Before carbamylation, 34% of the crises were induced by a concomitant illness, usually an infection. During carbamylation, the incidence of induced crises decreased 50% while spontaneous crises virtually disappeared. The marked improvements in hematologic parameters and the decreased frequency of severe painful crises observed during this study offer sufficient promise to warrant further exploration, hopefully using more efficient techniques, of the clinical efficacy of extracorporeal erythrocyte carbamylation in sickle cell anemia.", "contents": "Hematologic and clinical responses in patients with sickle cell anemia after chronic extracorporeal red cell carbamylation. In eight patients with sickle cell anemia, weekly extracorporeal carbamylation of about 20% of the circulating red cell mass was carried out for 2 yr or longer. At each visit, a mean of 1.3+/-0.2 mol of cyanate were incorporated per mole of hemoglobin in the carbamylated erythrocytes. Within 3 mo, a stable level of about 35-50% of the circulating erythrocytes was carbamylated. This quantity and degree of hemoglobin carbamylation produced a decrease in mean whole blood P50 from 33 to 26 mm Hg. During the first 3 mo of carbamylation, the mean hemoglobin increased from 6.4 to 9.1 g/100 ml, while mean absolute reticulocytes decreased by 58% and circulating irreversibly sickled erythrocytes decreased by 65%. The mean red cell life span increased from 13 days before treatment to 21.6 days after 3 mo of carbamylation. Beyond the 3rd mo of carbamylation, blood P50, hemoglobin, and reticulocytes remained quite stable. No toxic effects of extracorporeal carbamylation of erythrocytes were noted. The capacity of blood to release oxygen at 30 mm Hg PO2 increased from 4.3 to 5.0 cm3/100 ml blood during carbamylation. The overall frequency of severe painful crises decreased by about 80% during carbamylation. Before carbamylation, 34% of the crises were induced by a concomitant illness, usually an infection. During carbamylation, the incidence of induced crises decreased 50% while spontaneous crises virtually disappeared. The marked improvements in hematologic parameters and the decreased frequency of severe painful crises observed during this study offer sufficient promise to warrant further exploration, hopefully using more efficient techniques, of the clinical efficacy of extracorporeal erythrocyte carbamylation in sickle cell anemia.", "PMID": 956392}
{"id": "pubmed23n0032_2208", "title": "Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes.", "content": "The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human lymphocyte transformation and a detailed study of adenosine metabolism throughout phytohemagglutinin-induced blastogenesis. The adenosine deaminase inhibitor, coformycin, at a concentration that inhibited adenosine deaminase activity more than 95%, or 50 muM adenosine, did not prevent blastogenesis by criteria of morphology or thymidine incorporation into acid-precipitable material. The combination of coformycin and adenosine, however, substantially reduced both the viable cell count and the incorporation of thymidine into DNA in phytohemagglutinin-stimulated lymphocytes. Incubation of lymphocytes with phytohemagglutinin for 72 h produced a 12-fold increase in the rate of deamination and a 6-fold increase in phosphorylation of adenosine by intact lymphocytes. There was no change in the apparent affinity for adenosine with either deamination or phosphorylation. The increased rates of metabolism, apparent as early as 3 h after addition of mitogen, may be due to increased entry of the nucleoside into stimulated lymphocytes. Increased adenosine metabolism was not due to changes in total enzyme activity; after 72 h in culture, the ratios of specific activities in extracts of stimulated to unstimulated lymphocytes were essentially unchanged for adenosine kinase, 0.92, and decreased for adenosine deaminase, 0.44. As much as 38% of the initial lymphocyte adenosine deaminase activity accumulated extracellularly after a 72-h culture with phytohemagglutinin. In phytohemagglutinin-stimulated lymphocytes, the principal route of adenosine metabolism was phosphorylation at less than 5 muM adenosine, and deamination at concentrations greater than 5 muM. In unstimulated lymphocytes, deamination was the principal route of adenosine metabolism over the range of adenosine concentrations studied (0.5-250 muM). These studies demonstrate the dependence of both the unstimulated and stimulated lymphocyte on adenosine and may account for the observed sensitivity of mitogen-stimulated lymphocytes to the toxic effects of exogenously supplied adenosine in the presence of the adenosine deaminase inhibitor coformycin. A single case of immunodeficiency disease has been reported in association with purine nucleoside phosphorylase deficiency. The catabolism of guanosine was also found to be enhanced in stimulated normal lymphocytes; phosphorolysis of guanosine to guanine by intact lymphocytes increased six fold after 72-h culture with phytohemagglutinin. The specific activity of purine nucleoside phosphorylase in extracts, with guanosine as substrate, was essentially the same in stimulated and unstimulated lymphocytes after 72 h of culture.", "contents": "Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes. The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human lymphocyte transformation and a detailed study of adenosine metabolism throughout phytohemagglutinin-induced blastogenesis. The adenosine deaminase inhibitor, coformycin, at a concentration that inhibited adenosine deaminase activity more than 95%, or 50 muM adenosine, did not prevent blastogenesis by criteria of morphology or thymidine incorporation into acid-precipitable material. The combination of coformycin and adenosine, however, substantially reduced both the viable cell count and the incorporation of thymidine into DNA in phytohemagglutinin-stimulated lymphocytes. Incubation of lymphocytes with phytohemagglutinin for 72 h produced a 12-fold increase in the rate of deamination and a 6-fold increase in phosphorylation of adenosine by intact lymphocytes. There was no change in the apparent affinity for adenosine with either deamination or phosphorylation. The increased rates of metabolism, apparent as early as 3 h after addition of mitogen, may be due to increased entry of the nucleoside into stimulated lymphocytes. Increased adenosine metabolism was not due to changes in total enzyme activity; after 72 h in culture, the ratios of specific activities in extracts of stimulated to unstimulated lymphocytes were essentially unchanged for adenosine kinase, 0.92, and decreased for adenosine deaminase, 0.44. As much as 38% of the initial lymphocyte adenosine deaminase activity accumulated extracellularly after a 72-h culture with phytohemagglutinin. In phytohemagglutinin-stimulated lymphocytes, the principal route of adenosine metabolism was phosphorylation at less than 5 muM adenosine, and deamination at concentrations greater than 5 muM. In unstimulated lymphocytes, deamination was the principal route of adenosine metabolism over the range of adenosine concentrations studied (0.5-250 muM). These studies demonstrate the dependence of both the unstimulated and stimulated lymphocyte on adenosine and may account for the observed sensitivity of mitogen-stimulated lymphocytes to the toxic effects of exogenously supplied adenosine in the presence of the adenosine deaminase inhibitor coformycin. A single case of immunodeficiency disease has been reported in association with purine nucleoside phosphorylase deficiency. The catabolism of guanosine was also found to be enhanced in stimulated normal lymphocytes; phosphorolysis of guanosine to guanine by intact lymphocytes increased six fold after 72-h culture with phytohemagglutinin. The specific activity of purine nucleoside phosphorylase in extracts, with guanosine as substrate, was essentially the same in stimulated and unstimulated lymphocytes after 72 h of culture.", "PMID": 956393}
{"id": "pubmed23n0032_2209", "title": "A micropuncture study of the early phase of acute urate nephropathy.", "content": "The early pathophysiological changes in acute urate nephropathy were investigated in a rat model using micropuncture, clearance, and morphologic methods. Plasma urate was increased from 1.2 +/- 0.6 to 20.1 +/- 3.1 mg/100 ml (P less than 0.001). Urinary urate rose from 24.3 +/- 5.1 to 142.2 +/- 21.0 mg/100 ml (P less than 0.001). Renal plasma flow and glomerular filtration rate fell to 17 and 14% of control values, respectively, and urine flow rate decreased from 11.3 +/- 4.8 to 4.2 +/- 2.2 mul/min (all P less than 0.005) Superficial nephron filtration rate fell less than that of the whole kidney (70 vs. 86%). Both proximal and distal tubular pressures were increased from 10.6 to 26.1 mm Hg and from 7.2 to 24.7 mm Hg, respectively (P less than 0.005). Efferent arteriolar and peritubular capillary pressures were increased twofold. Vascular resistance beyond the peritubular capillaries increased from 4.8 X 10(9) to 21.6 X 10(9) dynes s/cm5. Extensive deposits of uric acid and urate were found in the tubular system and vasa recti from the corticomedullary junction to the tip of the papilla. It is concluded from these experiments that not only tubular obstruction in the collecting ducts, but also obstruction of the distal renal vasculature, are the primary early pathogenetic events in acute urate nephropathy.", "contents": "A micropuncture study of the early phase of acute urate nephropathy. The early pathophysiological changes in acute urate nephropathy were investigated in a rat model using micropuncture, clearance, and morphologic methods. Plasma urate was increased from 1.2 +/- 0.6 to 20.1 +/- 3.1 mg/100 ml (P less than 0.001). Urinary urate rose from 24.3 +/- 5.1 to 142.2 +/- 21.0 mg/100 ml (P less than 0.001). Renal plasma flow and glomerular filtration rate fell to 17 and 14% of control values, respectively, and urine flow rate decreased from 11.3 +/- 4.8 to 4.2 +/- 2.2 mul/min (all P less than 0.005) Superficial nephron filtration rate fell less than that of the whole kidney (70 vs. 86%). Both proximal and distal tubular pressures were increased from 10.6 to 26.1 mm Hg and from 7.2 to 24.7 mm Hg, respectively (P less than 0.005). Efferent arteriolar and peritubular capillary pressures were increased twofold. Vascular resistance beyond the peritubular capillaries increased from 4.8 X 10(9) to 21.6 X 10(9) dynes s/cm5. Extensive deposits of uric acid and urate were found in the tubular system and vasa recti from the corticomedullary junction to the tip of the papilla. It is concluded from these experiments that not only tubular obstruction in the collecting ducts, but also obstruction of the distal renal vasculature, are the primary early pathogenetic events in acute urate nephropathy.", "PMID": 956394}
{"id": "pubmed23n0032_2210", "title": "The cytotoxicity of leukocytes and lymphocytes from patients with rheumatoid arthritis for synovial cells.", "content": "Unseparated peripheral blood leukocytes obtained from patients with rheumatoid arthritis (RA) were cytotoxic for synovial cells. The cytotoxic reactions produced by RA leukocytes were more frequent and of greater magnitude than cytotoxicity induced by leukocytes from normal persons and patients with other diseases, primarily connective tissue diseases. Furthermore, the cytotoxic activity of RA leukocytes was greater for RA synovial cells than for nonrheumatoid synovial cells, in contrast to the cytotoxicity of other leukocytes, which did not discriminate between synovial cells according to their origin. Tests with purified lymphocytes showed that the cytotoxicity of unseparated leukocytes directed against RA synovial cells was due to lymphocyte cytotoxicity. These data are consistent with the possibility that sensitized lymphocytes from patients with RA recognize a distinctive antigen present on rheumatoid synovial cells.", "contents": "The cytotoxicity of leukocytes and lymphocytes from patients with rheumatoid arthritis for synovial cells. Unseparated peripheral blood leukocytes obtained from patients with rheumatoid arthritis (RA) were cytotoxic for synovial cells. The cytotoxic reactions produced by RA leukocytes were more frequent and of greater magnitude than cytotoxicity induced by leukocytes from normal persons and patients with other diseases, primarily connective tissue diseases. Furthermore, the cytotoxic activity of RA leukocytes was greater for RA synovial cells than for nonrheumatoid synovial cells, in contrast to the cytotoxicity of other leukocytes, which did not discriminate between synovial cells according to their origin. Tests with purified lymphocytes showed that the cytotoxicity of unseparated leukocytes directed against RA synovial cells was due to lymphocyte cytotoxicity. These data are consistent with the possibility that sensitized lymphocytes from patients with RA recognize a distinctive antigen present on rheumatoid synovial cells.", "PMID": 956395}
{"id": "pubmed23n0032_2211", "title": "Cytological and enzymological characterization of adult human adipocyte precursors in culture.", "content": "Cell strains were derived from the stromal-vascular fraction of human omental adipose tissue and grown in culture. Since the purpose of this study was to isolate adipocyte precursors from adults, the cells were obtained from nonobese patients 40-60 yr of age. After treatment of adipose tissue with collagenase, mature adipocytes were separated from stromal-vascular fraction cells, and cell strains of the latter replicated in culture with a doubling time of 40-60 h. They were initially fusiform; upon reaching monolayer confluency, they accumulated lipid and became rounder. Skin fibroblasts from the same patients and grown under the same culture conditions remained fusiform and did not accumulate lipid. The stromal-vascular fraction cells of adipose tissue may be fibroblasts with the potential to become adipocyte precursors. Subcellular preparations of the cells grown from the stromal-vascular fraction revealed lipoprotein lipase activity (characterized by such properties as inhibition by 1 M NaCl) that was not detectable in skin fibroblasts. The overall specific activity of the enzymes that catalyze triglyceride synthesis was 15 times higher and that of fatty acid synthetase was 2 times higher in the cells cultured from the stromal-vascular fraction. The difference was significant in each case. Conversely, when isolated mature adipocytes were cultured, they lost considerable lipid and acquired morphological characteristics similar to those of stromal-vascular fraction cells. Thus, adipose tissue stromal-vascular fraction cells acquire in culture many of the morphological and enzymological characteristics of mature fat cells.", "contents": "Cytological and enzymological characterization of adult human adipocyte precursors in culture. Cell strains were derived from the stromal-vascular fraction of human omental adipose tissue and grown in culture. Since the purpose of this study was to isolate adipocyte precursors from adults, the cells were obtained from nonobese patients 40-60 yr of age. After treatment of adipose tissue with collagenase, mature adipocytes were separated from stromal-vascular fraction cells, and cell strains of the latter replicated in culture with a doubling time of 40-60 h. They were initially fusiform; upon reaching monolayer confluency, they accumulated lipid and became rounder. Skin fibroblasts from the same patients and grown under the same culture conditions remained fusiform and did not accumulate lipid. The stromal-vascular fraction cells of adipose tissue may be fibroblasts with the potential to become adipocyte precursors. Subcellular preparations of the cells grown from the stromal-vascular fraction revealed lipoprotein lipase activity (characterized by such properties as inhibition by 1 M NaCl) that was not detectable in skin fibroblasts. The overall specific activity of the enzymes that catalyze triglyceride synthesis was 15 times higher and that of fatty acid synthetase was 2 times higher in the cells cultured from the stromal-vascular fraction. The difference was significant in each case. Conversely, when isolated mature adipocytes were cultured, they lost considerable lipid and acquired morphological characteristics similar to those of stromal-vascular fraction cells. Thus, adipose tissue stromal-vascular fraction cells acquire in culture many of the morphological and enzymological characteristics of mature fat cells.", "PMID": 956396}
{"id": "pubmed23n0032_2212", "title": "Neutrophil kinetics in man.", "content": "A method has been developed for measuring neutrophil cellularity in normal human bone marrow, in which the neutrophil-erythroid ratio was determined from marrow sections and marrow normoblasts were estimated by the erythron iron turnover. Neutrophil maturational categories, defined by morphologic criteria, were supported by autoradiographs of marrow flashed-labeled with 3H-thymidine. Correction for multiple counting error was empirically derived by counting serial sections through cells of each maturational category. The normal neutrophil-erythroid ratio in 13 normal human subjects was 1.5 +/- 0.07. The mean number of normoblasts in the same subjects was estimated to be 5.07 +/- 0.84 X 10(9) cells/kg. Total marrow neutrophils (X 10(9) cells/kg) were 7.70 +/- 1.20, the postmitotic pool (metamyelocytes, bands, and segmented forms) was 5.59 +/- 0.90 and the mitotic pool (promyelocytes + myelocytes) was 2.11 +/- 0.36. Marrow neutrophil (\"total\") production has been determined from the number of neutrophils comprising the postmitotic marrow pool divided by their transit time Transit time was derived from the appearance in circulating neutrophils of injected 3H-thymidine. The postmitotic pool comprised 5.59 +/- 0.90 X 10(9) neutrophils/kg, and the transit time was 6.60 +/- 0.03 days. From these data marrow neutrophil production was calculated to be 0.85 X 10(9) cells/kg per day. Effective production, measured as the turnover of circulating neutrophils labeled with 3H-thymidine, was 0.87 +/- 0.13 X 10(9) cells/kg per day. This value correlated well with the calculation of marrow neutrophil production. A larger turnover of 1.62 +/- 0.46 X 10(9) cells/kg per day was obtained when diisopropylfluorophosphate-32P was used to label circulating neutrophils. Studies using isologous cells doubly labeled with 3H-thymidine and diisopropylfluorophosphate-32P demonstrated a lower recovery and shorter t1/2 of the 32P label.", "contents": "Neutrophil kinetics in man. A method has been developed for measuring neutrophil cellularity in normal human bone marrow, in which the neutrophil-erythroid ratio was determined from marrow sections and marrow normoblasts were estimated by the erythron iron turnover. Neutrophil maturational categories, defined by morphologic criteria, were supported by autoradiographs of marrow flashed-labeled with 3H-thymidine. Correction for multiple counting error was empirically derived by counting serial sections through cells of each maturational category. The normal neutrophil-erythroid ratio in 13 normal human subjects was 1.5 +/- 0.07. The mean number of normoblasts in the same subjects was estimated to be 5.07 +/- 0.84 X 10(9) cells/kg. Total marrow neutrophils (X 10(9) cells/kg) were 7.70 +/- 1.20, the postmitotic pool (metamyelocytes, bands, and segmented forms) was 5.59 +/- 0.90 and the mitotic pool (promyelocytes + myelocytes) was 2.11 +/- 0.36. Marrow neutrophil (\"total\") production has been determined from the number of neutrophils comprising the postmitotic marrow pool divided by their transit time Transit time was derived from the appearance in circulating neutrophils of injected 3H-thymidine. The postmitotic pool comprised 5.59 +/- 0.90 X 10(9) neutrophils/kg, and the transit time was 6.60 +/- 0.03 days. From these data marrow neutrophil production was calculated to be 0.85 X 10(9) cells/kg per day. Effective production, measured as the turnover of circulating neutrophils labeled with 3H-thymidine, was 0.87 +/- 0.13 X 10(9) cells/kg per day. This value correlated well with the calculation of marrow neutrophil production. A larger turnover of 1.62 +/- 0.46 X 10(9) cells/kg per day was obtained when diisopropylfluorophosphate-32P was used to label circulating neutrophils. Studies using isologous cells doubly labeled with 3H-thymidine and diisopropylfluorophosphate-32P demonstrated a lower recovery and shorter t1/2 of the 32P label.", "PMID": 956397}
{"id": "pubmed23n0032_2213", "title": "Composition of weight lost during short-term weight reduction. Metabolic responses of obese subjects to starvation and low-calorie ketogenic and nonketogenic diets.", "content": "The effects of starvation, an 800-kcal mixed diet and an 800-kcal ketogenic (low carbohydrate-high fat) diet on the composition of weight lost were determined in each of six obese subjects during three 10-day periods. The energy-nitrogen balance method was used to quantify the three measurable components of weight loss; protein, fat, and water. On the 800-kcal ketogenic diet, subjects lost (mean +/- SE) 466.6 +/-51.3 g/day; on the isocaloric mixed diet, which provided carbohydrate and fat in conventional proportions, they lost 277.9+/- 32.1 g/day. Composition of weight lost (percentage) during the ketogenic diet was water 61.2, fat 35.0, protein 3.8. During the mixed diet, composition of loss was water 37.1, fat 59.5, protein 3.4...", "contents": "Composition of weight lost during short-term weight reduction. Metabolic responses of obese subjects to starvation and low-calorie ketogenic and nonketogenic diets. The effects of starvation, an 800-kcal mixed diet and an 800-kcal ketogenic (low carbohydrate-high fat) diet on the composition of weight lost were determined in each of six obese subjects during three 10-day periods. The energy-nitrogen balance method was used to quantify the three measurable components of weight loss; protein, fat, and water. On the 800-kcal ketogenic diet, subjects lost (mean +/- SE) 466.6 +/-51.3 g/day; on the isocaloric mixed diet, which provided carbohydrate and fat in conventional proportions, they lost 277.9+/- 32.1 g/day. Composition of weight lost (percentage) during the ketogenic diet was water 61.2, fat 35.0, protein 3.8. During the mixed diet, composition of loss was water 37.1, fat 59.5, protein 3.4...", "PMID": 956398}
{"id": "pubmed23n0032_2214", "title": "Heterogeneity of plasma glucagon. Circulating components in normal subjects and patients with chronic renal failure.", "content": "Plasma immunoreactive glucagon (IRG) concentrations were measured in 36 patients with chronic renal failure (CRF) and 32 normal subjects. In addition, the components of circulating IRG were analyzed by gel filtration in the fasting state and after physiological stimuli. Fasting IRG was elevated (P less than 0.001) in CRF patients (534 +/- 32 pg/ml) compared with the levels found in healthy subjects (113 +/- 9 pg/ml). Oral glucose suppressed plasma IRG in CRF patients from a basal level of 568 +/- 52 to a nadir of 354 +/- 57 pg/ml (120 min). This degree of suppression (38%) was comparable to that found in normal subjects (basal = 154 +/- 20 to 100 +/- 23 pg/ml) at 120 min (35%). Intravenous infusion of arginine (250 mg/kg) resulted in a 71% rise in IRG in CRF patients and a 166% increase in normal subjects. Gel filtration of fasting plasma from CRF patients showed three major peaks. The earliest (A) was found in the void volume (mol wt greater than 40,000) and constituted 16.5 +/- 4.7% of the elution profile. The middle peak (B) eluted just beyond the proinsulin marker (approximately 9,000 mol wt) and constituted the largest proportion of the elution profile (56.5 +/- 3.4%). The third peak (C) coincided with the standard glucagon and [125I]glucagon markers (3,485 mol wt) and comprised 27.0 +/- 4% of the IRG profile. In contrast, only peaks A and C were found in fasting plasma of normal subjects (53.6 +/- 10.4% in A and 46.4 +/- 10.4 in C). After oral glucose, glucagon immunoreactivity in the 3,500 mol wt peak (C) was markedly suppressed, while the B peak in patients with CRF declined to a lesser extent. The A peak in both groups was unchanged. After an arginine infusion only the C peak increased in both groups of subjects. Gel filtration of plasma in 3 M acetic acid gave similar profiles to those obtained in glycine albumin buffer. Exposure of serum to trypsin indicated that the B and C peaks were digestible, while the A peak was resistant to the action of the enzyme. In one sample, peak C increased after a 2-h exposure of serum to trypsin. We conclude that circulating IRG in normal subjects and patients with CRF is heterogenous. The hyperglucagonemia of renal failure is largely due to an increase in IRG material of approximately 9,000 mol wt, consistent with proglucagon, although the 3,500 mol wt component is also considerably elevated (threefold). The significance of circulating IRG levels should be interpreted with caution until the relative biological activity of the three components is established.", "contents": "Heterogeneity of plasma glucagon. Circulating components in normal subjects and patients with chronic renal failure. Plasma immunoreactive glucagon (IRG) concentrations were measured in 36 patients with chronic renal failure (CRF) and 32 normal subjects. In addition, the components of circulating IRG were analyzed by gel filtration in the fasting state and after physiological stimuli. Fasting IRG was elevated (P less than 0.001) in CRF patients (534 +/- 32 pg/ml) compared with the levels found in healthy subjects (113 +/- 9 pg/ml). Oral glucose suppressed plasma IRG in CRF patients from a basal level of 568 +/- 52 to a nadir of 354 +/- 57 pg/ml (120 min). This degree of suppression (38%) was comparable to that found in normal subjects (basal = 154 +/- 20 to 100 +/- 23 pg/ml) at 120 min (35%). Intravenous infusion of arginine (250 mg/kg) resulted in a 71% rise in IRG in CRF patients and a 166% increase in normal subjects. Gel filtration of fasting plasma from CRF patients showed three major peaks. The earliest (A) was found in the void volume (mol wt greater than 40,000) and constituted 16.5 +/- 4.7% of the elution profile. The middle peak (B) eluted just beyond the proinsulin marker (approximately 9,000 mol wt) and constituted the largest proportion of the elution profile (56.5 +/- 3.4%). The third peak (C) coincided with the standard glucagon and [125I]glucagon markers (3,485 mol wt) and comprised 27.0 +/- 4% of the IRG profile. In contrast, only peaks A and C were found in fasting plasma of normal subjects (53.6 +/- 10.4% in A and 46.4 +/- 10.4 in C). After oral glucose, glucagon immunoreactivity in the 3,500 mol wt peak (C) was markedly suppressed, while the B peak in patients with CRF declined to a lesser extent. The A peak in both groups was unchanged. After an arginine infusion only the C peak increased in both groups of subjects. Gel filtration of plasma in 3 M acetic acid gave similar profiles to those obtained in glycine albumin buffer. Exposure of serum to trypsin indicated that the B and C peaks were digestible, while the A peak was resistant to the action of the enzyme. In one sample, peak C increased after a 2-h exposure of serum to trypsin. We conclude that circulating IRG in normal subjects and patients with CRF is heterogenous. The hyperglucagonemia of renal failure is largely due to an increase in IRG material of approximately 9,000 mol wt, consistent with proglucagon, although the 3,500 mol wt component is also considerably elevated (threefold). The significance of circulating IRG levels should be interpreted with caution until the relative biological activity of the three components is established.", "PMID": 956399}
{"id": "pubmed23n0032_2215", "title": "Hemodynamic determinants of the time-course of fall in canine left ventricular pressure.", "content": "The hemodynamic determinants of the time-course of fall in isovolumic left ventricular pressure were assessed in isolated canine left ventricular preparations. Pressure fall was studied in isovolumic beats or during prolonged isovolumic diastole after ejection. Pressure fall was studied in isovolumic relaxation for isovolumic and ejecting beats (r less than or equal to 0.98) and was therefore characterized by a time constant, T. Higher heart rates shortened T slightly from 52.6 +/- 4.5 ms at 110/min to 48.2 +/- 6.0 ms at 160/min (P less than 0.01, n = 8). Higher ventricular volumes under isovolumic conditions resulted in higher peak left ventricular pressure but no significant change in T. T did shorten from 67.1 +/- 5.0 ms in isovolumic beats to 45.8 +/- 2.9 ms in the ejecting beats (P less than 0.001, n = 14). In the ejecting beats, peak systolic pressure was lower, and end-systolic volume smaller. To differentiate the effects of systolic shortening during ejection from those of lower systolic pressure and smaller end-systolic volume, beats with large end-diastolic volumes were compared to beats with smaller end-diastolic volumes. The beats with smaller end-diastolic volumes exhibited less shortening but similar end-systolic volumes and peak systolic pressure. T again shortened to a greater extent in the beats with greater systolic shortening. Calcium chloride and acetylstrophanthidin resulted in no significant change in T, but norepinephrine, which accelerates active relaxation, resulted in a significant shortening of T (65.6 +/- 13.4 vs. 46.3 +/- 7.0 ms, P less than 0.02). During recovery from ischemia, T increased significantly from 59.3 +/- 9.6 to 76.8 +/- 13.1 ms when compared with the preischemic control beat (P less than 0.05). Thus, the present studies show that the time-course of isovolumic pressure fall subsequent to maximum negative dP/dt is exponential, independent of systolic stress and end-systolic fiber length, and minimally dependent on heart rate. T may be an index of the activity of the active cardiac relaxing system and appears dependent on systolic fiber shortening.", "contents": "Hemodynamic determinants of the time-course of fall in canine left ventricular pressure. The hemodynamic determinants of the time-course of fall in isovolumic left ventricular pressure were assessed in isolated canine left ventricular preparations. Pressure fall was studied in isovolumic beats or during prolonged isovolumic diastole after ejection. Pressure fall was studied in isovolumic relaxation for isovolumic and ejecting beats (r less than or equal to 0.98) and was therefore characterized by a time constant, T. Higher heart rates shortened T slightly from 52.6 +/- 4.5 ms at 110/min to 48.2 +/- 6.0 ms at 160/min (P less than 0.01, n = 8). Higher ventricular volumes under isovolumic conditions resulted in higher peak left ventricular pressure but no significant change in T. T did shorten from 67.1 +/- 5.0 ms in isovolumic beats to 45.8 +/- 2.9 ms in the ejecting beats (P less than 0.001, n = 14). In the ejecting beats, peak systolic pressure was lower, and end-systolic volume smaller. To differentiate the effects of systolic shortening during ejection from those of lower systolic pressure and smaller end-systolic volume, beats with large end-diastolic volumes were compared to beats with smaller end-diastolic volumes. The beats with smaller end-diastolic volumes exhibited less shortening but similar end-systolic volumes and peak systolic pressure. T again shortened to a greater extent in the beats with greater systolic shortening. Calcium chloride and acetylstrophanthidin resulted in no significant change in T, but norepinephrine, which accelerates active relaxation, resulted in a significant shortening of T (65.6 +/- 13.4 vs. 46.3 +/- 7.0 ms, P less than 0.02). During recovery from ischemia, T increased significantly from 59.3 +/- 9.6 to 76.8 +/- 13.1 ms when compared with the preischemic control beat (P less than 0.05). Thus, the present studies show that the time-course of isovolumic pressure fall subsequent to maximum negative dP/dt is exponential, independent of systolic stress and end-systolic fiber length, and minimally dependent on heart rate. T may be an index of the activity of the active cardiac relaxing system and appears dependent on systolic fiber shortening.", "PMID": 956400}
{"id": "pubmed23n0032_2216", "title": "Influence of physiologic hyperglucagonemia on basal and insulin-inhibited splanchnic glucose output in normal man.", "content": "To evaluate the effects of physiologic hyperglucagonemia on splanchnic glucose output, glucagon was infused in a dose of 3 ng/kg per min to healthy subjects in the basal state and after splanchnic glucose output had been inhibited by an infusion of glucose (2 mg/kg per min). In the basal state, infusion of glucagon causing a 309 +/- 25 pg/ml rise in plasma concentration was accompanied by a rapid increase in splanchnic glucose output to values two to three times basal by 7-15 min. The rise in arterial blood glucose (0.5-1.5 mM) correlated directly with the increment in splanchnic glucose output. Despite continued glucagon infusion, and in the face of stable insulin levels, splanchnic glucose output declined after 22 min, returning to basal levels by 30-45 min. In the subjects initially receiving the glucose infusion, arterial insulin concentration rose by 5-12 muU/ml, while splanchnic glucose output fell by 85-100%. Infusion of glucagon causing an increment in plasma glucagon concentration of 272 +/- 30 pg/ml reversed the inhibition in splanchnic glucose production within 5 min. Splanchnic glucose output reached a peak increment 60% above basal levels at 10 min, and subsequently declined to levels 20-25% below basal at 30-45 min. These findings provide direct evidence that physiologic increments in plasma glucagon stimulate splanchnic glucose output in the basal state and reverse insulin-mediated inhibition of splanchnic glucose production in normal man. The transient nature of the stimulatory effect of glucagon on splanchnic glucose output suggests the rapid development of inhibition or reversal of glucagon action. This inhibition does not appear to depend on increased insulin secretio.", "contents": "Influence of physiologic hyperglucagonemia on basal and insulin-inhibited splanchnic glucose output in normal man. To evaluate the effects of physiologic hyperglucagonemia on splanchnic glucose output, glucagon was infused in a dose of 3 ng/kg per min to healthy subjects in the basal state and after splanchnic glucose output had been inhibited by an infusion of glucose (2 mg/kg per min). In the basal state, infusion of glucagon causing a 309 +/- 25 pg/ml rise in plasma concentration was accompanied by a rapid increase in splanchnic glucose output to values two to three times basal by 7-15 min. The rise in arterial blood glucose (0.5-1.5 mM) correlated directly with the increment in splanchnic glucose output. Despite continued glucagon infusion, and in the face of stable insulin levels, splanchnic glucose output declined after 22 min, returning to basal levels by 30-45 min. In the subjects initially receiving the glucose infusion, arterial insulin concentration rose by 5-12 muU/ml, while splanchnic glucose output fell by 85-100%. Infusion of glucagon causing an increment in plasma glucagon concentration of 272 +/- 30 pg/ml reversed the inhibition in splanchnic glucose production within 5 min. Splanchnic glucose output reached a peak increment 60% above basal levels at 10 min, and subsequently declined to levels 20-25% below basal at 30-45 min. These findings provide direct evidence that physiologic increments in plasma glucagon stimulate splanchnic glucose output in the basal state and reverse insulin-mediated inhibition of splanchnic glucose production in normal man. The transient nature of the stimulatory effect of glucagon on splanchnic glucose output suggests the rapid development of inhibition or reversal of glucagon action. This inhibition does not appear to depend on increased insulin secretio.", "PMID": 956401}
{"id": "pubmed23n0032_2217", "title": "Arterial wall lesions after pulmonary embolism, especially ruptures and aneurysms.", "content": "During an histological study of pulmonary thromboembolism, arterial wall splits, many associated with saccular microaneurysms, were observed in association with emboli or their fibrous residue. Other aneurysmal lesions, non-inflammatory focal medial necrosis, and medial scars were also seen in a few cases. The nature of the arterial rents indicates that they arose by mechanical splitting after acute stretching of the artery at the time of embolic impaction. Saccular aneurysms then developed in some. Impaction could also have caused the foci of medial necrosis while the medial scars could represent healed former necrosis of rupture. Though the observed incidence of lesions was relatively low, this seems to be due to their small sizes and the sampling inherent in conventional histological analysis. It is concluded that arterial splits are probably a common effect of pulmonary embolism and often give rise to small aneurysms.", "contents": "Arterial wall lesions after pulmonary embolism, especially ruptures and aneurysms. During an histological study of pulmonary thromboembolism, arterial wall splits, many associated with saccular microaneurysms, were observed in association with emboli or their fibrous residue. Other aneurysmal lesions, non-inflammatory focal medial necrosis, and medial scars were also seen in a few cases. The nature of the arterial rents indicates that they arose by mechanical splitting after acute stretching of the artery at the time of embolic impaction. Saccular aneurysms then developed in some. Impaction could also have caused the foci of medial necrosis while the medial scars could represent healed former necrosis of rupture. Though the observed incidence of lesions was relatively low, this seems to be due to their small sizes and the sampling inherent in conventional histological analysis. It is concluded that arterial splits are probably a common effect of pulmonary embolism and often give rise to small aneurysms.", "PMID": 956448}
{"id": "pubmed23n0032_2218", "title": "Development of germinal centres in the spleen in infants related to birth and unexpected death.", "content": "The development of germinal centres in the spleen has been estimated in a series of 266 children dying in hospital and 205 children presenting as unexpected death in infancy. The prevalence of germinal centres in the spleen increases rapidly after birth. The percentage of Malpighian bodies showing germinal centres in the spleen is greater in all categories of our classification of death in children dying unexpectedly at home when these are compared with hospital deaths. It is concluded that the children presenting as unexpected death in infancy show no evidence of any deficiency in cellular immunological response and may well have been subjected to increased or precocious antigenic stimulation.", "contents": "Development of germinal centres in the spleen in infants related to birth and unexpected death. The development of germinal centres in the spleen has been estimated in a series of 266 children dying in hospital and 205 children presenting as unexpected death in infancy. The prevalence of germinal centres in the spleen increases rapidly after birth. The percentage of Malpighian bodies showing germinal centres in the spleen is greater in all categories of our classification of death in children dying unexpectedly at home when these are compared with hospital deaths. It is concluded that the children presenting as unexpected death in infancy show no evidence of any deficiency in cellular immunological response and may well have been subjected to increased or precocious antigenic stimulation.", "PMID": 956449}
{"id": "pubmed23n0032_2219", "title": "Carcinomas and carcinoid tumours of the appendix in a district general hospital.", "content": "In a retrospective series of 40 appendiceal tumours occurring over a 10-year period, 30 were carcinoid tumours of classical histological pattern, five were adenocarcinomas, and two were tumours intermediate in pattern between carcinoid and carcinoma. The remaining three tumours were secondaries from primary colonic tumours. The implications of the findings are discussed.", "contents": "Carcinomas and carcinoid tumours of the appendix in a district general hospital. In a retrospective series of 40 appendiceal tumours occurring over a 10-year period, 30 were carcinoid tumours of classical histological pattern, five were adenocarcinomas, and two were tumours intermediate in pattern between carcinoid and carcinoma. The remaining three tumours were secondaries from primary colonic tumours. The implications of the findings are discussed.", "PMID": 956450}
{"id": "pubmed23n0032_2220", "title": "Effect of methotrexate therapy in psoriatics on the Ito cells in liver biopsies, assessed by point-counting.", "content": "To evaluate the relationship, both quantitative and qualitative, between the Ito cells and methotrexate (MTX) therapy Ito cells were studied by light microscopy in 1 mum toluidine blue stained sections and by electron microscopy in 24 pairs of Menghini needle biopsies before and after MTX therapy of 24 consenting patients with severe psoriasis. Light microscopy showed a statistically significant increase in pathological findings (P less than 0-05) and in the number of Ito cells and their size (P less than 0-0001) after MTX therapy. It was not possible to show a statistically significant correlation between the increase in the number of Ito cells and the cumulative dose of MTX. Ultrastructural analysis of Ito cells showed no marked difference from pre to post-MTX specimens. The fibrosis and cirrhosis seen after MTX therapy in some liver biopsies from psoriatics and the post-MTX increase in the number of Ito cells direct attention to the possible role of Ito cells as fibroblast precursors.", "contents": "Effect of methotrexate therapy in psoriatics on the Ito cells in liver biopsies, assessed by point-counting. To evaluate the relationship, both quantitative and qualitative, between the Ito cells and methotrexate (MTX) therapy Ito cells were studied by light microscopy in 1 mum toluidine blue stained sections and by electron microscopy in 24 pairs of Menghini needle biopsies before and after MTX therapy of 24 consenting patients with severe psoriasis. Light microscopy showed a statistically significant increase in pathological findings (P less than 0-05) and in the number of Ito cells and their size (P less than 0-0001) after MTX therapy. It was not possible to show a statistically significant correlation between the increase in the number of Ito cells and the cumulative dose of MTX. Ultrastructural analysis of Ito cells showed no marked difference from pre to post-MTX specimens. The fibrosis and cirrhosis seen after MTX therapy in some liver biopsies from psoriatics and the post-MTX increase in the number of Ito cells direct attention to the possible role of Ito cells as fibroblast precursors.", "PMID": 956451}
{"id": "pubmed23n0032_2221", "title": "Computerization of a large antenatal serology service.", "content": "A computer scheme for a large antenatal serology service is described. A primary request form (for mother, father or child) has been developed. Standard reports are produced by the computer and are issued with a tear-off slip which should accompany subsequent specimens. Complicated reports are made by special letter. An instruction booklet is issued to all users. Information on patients and clinically significant antibodies is held in a special computer file and is regularly updated. The system provides an efficient and reliable day-to-day service and readily accessible data for retrospective research. A brief note on the computer elements of the system is provided.", "contents": "Computerization of a large antenatal serology service. A computer scheme for a large antenatal serology service is described. A primary request form (for mother, father or child) has been developed. Standard reports are produced by the computer and are issued with a tear-off slip which should accompany subsequent specimens. Complicated reports are made by special letter. An instruction booklet is issued to all users. Information on patients and clinically significant antibodies is held in a special computer file and is regularly updated. The system provides an efficient and reliable day-to-day service and readily accessible data for retrospective research. A brief note on the computer elements of the system is provided.", "PMID": 956452}
{"id": "pubmed23n0032_2222", "title": "Anisocytosis and the C-1000 Channelyzer in macrocytic anaemia.", "content": "Red cell anisocytosis as assessed using the Coulter Channelyzer C-1000 showed an increase with progressive anaemia in 25 patients with macrocytosis due to B12 and/or folate deficiency. In deficiency of a single factor, the degree of anisocytosis increased with progressive anaemia. In five cases with B12 and folate deficiency combined, anisocytosis was markedly increased out of proportion to the degree of anaemia present. Iron stores were also reduced in four of these cases. It is suggested that objective measurement of anisocytosis is of early diagnostic value in the assessment of multiple haematinic factor deficiency, for example, in macrocytic anaemia associated with malabsorption states and unexpected multiple deficiency states.", "contents": "Anisocytosis and the C-1000 Channelyzer in macrocytic anaemia. Red cell anisocytosis as assessed using the Coulter Channelyzer C-1000 showed an increase with progressive anaemia in 25 patients with macrocytosis due to B12 and/or folate deficiency. In deficiency of a single factor, the degree of anisocytosis increased with progressive anaemia. In five cases with B12 and folate deficiency combined, anisocytosis was markedly increased out of proportion to the degree of anaemia present. Iron stores were also reduced in four of these cases. It is suggested that objective measurement of anisocytosis is of early diagnostic value in the assessment of multiple haematinic factor deficiency, for example, in macrocytic anaemia associated with malabsorption states and unexpected multiple deficiency states.", "PMID": 956453}
{"id": "pubmed23n0032_2223", "title": "Regional quality control.", "content": "Methods of quality control of some major haematological techniques are described. These methods have been applied in haematology laboratories serving a population of 2 million using existing facilities for preparation and transport of the necessary materials.", "contents": "Regional quality control. Methods of quality control of some major haematological techniques are described. These methods have been applied in haematology laboratories serving a population of 2 million using existing facilities for preparation and transport of the necessary materials.", "PMID": 956454}
{"id": "pubmed23n0032_2224", "title": "Comparison of uridine uptake at 24 hours with thymidine uptake at 72 hours in phytohaemagglutinin-stimulated cultures of pregnant and other subjects.", "content": "As a possible more rapid test of lymphocyte reactivity to phytohaemagglutinin (PHA) than the standard estimation of thymidine uptake after 72 hours (T72), uridine uptake after 24 hours (U24) was studied in a total of 96 individuals. No reliable correlation between the two estimations was found in 29 outpatients showing a wide range of reactivity to PHA nor in 30 healthy control subjects nor in 16 pregnant subjects. Conflicting reports have appeared in the literature as to whether lymphocyte reactivity to PHA is normal, depressed or enhanced in pregnancy. Our group of pregnant subjects showed a significant depression of both U24 (P less than 0-01) and T72 (P less than 0-02) results as compared to normal female controls. Cultures of semipurified lymphocytes prepared by gradient centrifugation on a further 21 normal control subjects showed no better correlation between U24 and T72 results than the above unpurified-leucocyte cultures.", "contents": "Comparison of uridine uptake at 24 hours with thymidine uptake at 72 hours in phytohaemagglutinin-stimulated cultures of pregnant and other subjects. As a possible more rapid test of lymphocyte reactivity to phytohaemagglutinin (PHA) than the standard estimation of thymidine uptake after 72 hours (T72), uridine uptake after 24 hours (U24) was studied in a total of 96 individuals. No reliable correlation between the two estimations was found in 29 outpatients showing a wide range of reactivity to PHA nor in 30 healthy control subjects nor in 16 pregnant subjects. Conflicting reports have appeared in the literature as to whether lymphocyte reactivity to PHA is normal, depressed or enhanced in pregnancy. Our group of pregnant subjects showed a significant depression of both U24 (P less than 0-01) and T72 (P less than 0-02) results as compared to normal female controls. Cultures of semipurified lymphocytes prepared by gradient centrifugation on a further 21 normal control subjects showed no better correlation between U24 and T72 results than the above unpurified-leucocyte cultures.", "PMID": 956455}
{"id": "pubmed23n0032_2225", "title": "Infection with minute-colony-forming beta-haemolytic streptococci.", "content": "One hundred and thirty-one strains of minute-colony-forming beta-haemolytic streptococci were isolated during the course of routine investigations in clinical bacteriology. Each strain was examined for the presence of polysaccharide antigens of Lancefield's groups A, C, G, and F and characterized in detail as to biochemical and cultural features. On the results of these tests it is concluded that the strains should be placed in the species Streptococcus milleri. The clinical details relevant to the various strains are summarized according to the site of isolation, and their pathological significance is discussed.", "contents": "Infection with minute-colony-forming beta-haemolytic streptococci. One hundred and thirty-one strains of minute-colony-forming beta-haemolytic streptococci were isolated during the course of routine investigations in clinical bacteriology. Each strain was examined for the presence of polysaccharide antigens of Lancefield's groups A, C, G, and F and characterized in detail as to biochemical and cultural features. On the results of these tests it is concluded that the strains should be placed in the species Streptococcus milleri. The clinical details relevant to the various strains are summarized according to the site of isolation, and their pathological significance is discussed.", "PMID": 956456}
{"id": "pubmed23n0032_2226", "title": "Assay of gentamicin in cerebrospinal fluid.", "content": "A comparison of standard curves obtained from a conventional plate diffusion assay method revealed significant differences when gentamicin standards were made up in different media. Standards made up in distilled water resulted in a curve which differed from that of standards made up in pooled human cerebrospinal fluid by a factor of up to 4. When the assay medium was supplemented with 0-5% sodium chloride, the difference between the two standard curves was reduced to a factor of about 1-5. The curve obtained from standards made up in 150 mM sodium chloride/4-5 mM calcium chloride correlated well with that from standards made up in cerebrospinal fluid. There was no evidence of gentamicin being bound to protein in the cerebrospinal fluid.", "contents": "Assay of gentamicin in cerebrospinal fluid. A comparison of standard curves obtained from a conventional plate diffusion assay method revealed significant differences when gentamicin standards were made up in different media. Standards made up in distilled water resulted in a curve which differed from that of standards made up in pooled human cerebrospinal fluid by a factor of up to 4. When the assay medium was supplemented with 0-5% sodium chloride, the difference between the two standard curves was reduced to a factor of about 1-5. The curve obtained from standards made up in 150 mM sodium chloride/4-5 mM calcium chloride correlated well with that from standards made up in cerebrospinal fluid. There was no evidence of gentamicin being bound to protein in the cerebrospinal fluid.", "PMID": 956457}
{"id": "pubmed23n0032_2227", "title": "Regeneration of retinal axons into the goldfish optic tectum.", "content": "The growth of regenerating retinal axons into the central portion of the optic tectum of adult goldfish was examined with the light and electron microscopes. Optic tracts were cut and, two days to five months later, the animals were perfused and the tecta prepared for microscopy. Regenerating axons first reached central regions of the tectum seven to ten days postoperatively. Regenerating axons appear in very large numbers and travel in fascicles in the stratum opticum (SO) and in the adjacent neuropil, the stratum fibrosum et griseum superficiale (SFGS). In the SO, the fascicles are bordered by glial cells and degenerating debris. Within the SFGS, however, the fascicles do not seem to be similarly associated with glial cells and degenerating debris. The youngest regenerating axons are very slender processes, containing microtubules but few or no neurofilaments or dense granular material. By 10 to 14 days postoperatively, neurofilaments can be seen and, in addition, large numbers of vesicles with dense cores appear. The vesicles with dense cores increase in numbers until about 28 days postoperatively and then become quite rare. That vesicles with dense cores were seen in regenerating axons in both SO and SFGS during the period of growth into the tectum but were not seen in axon terminals at any time, suggests that they may be concerned with axon elongation. During the period one month to five months postoperatively, the regenerating axons gradually increase in diameter but do not reach preoperative sizes, suggesting that the regenerative changes may still be occurring. Remyelination is delayed and proceeds slowly. Many axons remain unmyelinated for as long as five months postoperatively.", "contents": "Regeneration of retinal axons into the goldfish optic tectum. The growth of regenerating retinal axons into the central portion of the optic tectum of adult goldfish was examined with the light and electron microscopes. Optic tracts were cut and, two days to five months later, the animals were perfused and the tecta prepared for microscopy. Regenerating axons first reached central regions of the tectum seven to ten days postoperatively. Regenerating axons appear in very large numbers and travel in fascicles in the stratum opticum (SO) and in the adjacent neuropil, the stratum fibrosum et griseum superficiale (SFGS). In the SO, the fascicles are bordered by glial cells and degenerating debris. Within the SFGS, however, the fascicles do not seem to be similarly associated with glial cells and degenerating debris. The youngest regenerating axons are very slender processes, containing microtubules but few or no neurofilaments or dense granular material. By 10 to 14 days postoperatively, neurofilaments can be seen and, in addition, large numbers of vesicles with dense cores appear. The vesicles with dense cores increase in numbers until about 28 days postoperatively and then become quite rare. That vesicles with dense cores were seen in regenerating axons in both SO and SFGS during the period of growth into the tectum but were not seen in axon terminals at any time, suggests that they may be concerned with axon elongation. During the period one month to five months postoperatively, the regenerating axons gradually increase in diameter but do not reach preoperative sizes, suggesting that the regenerative changes may still be occurring. Remyelination is delayed and proceeds slowly. Many axons remain unmyelinated for as long as five months postoperatively.", "PMID": 956460}
{"id": "pubmed23n0032_2228", "title": "Reconstruction of neurons of dorsal horn proper using Golgi-stained serial sections.", "content": "Three neurons located in the dorsal horn (Lamina III) have been reconstructed using Golgi-stained serial sections. These cells including dendritic and axonal branchings appear to remain within the dorsal horn and to belong to small-sized local interneurons of the dorsal horn proper. The measurements have been made with various criteria: the medio-lateral, dorso-ventral and rostro-caudal extents of the dendritic tree as well as the axonal branchings, total dendritic and axonal length, number of axonal endings and distance from the starting point of the axon at the dendrite to the point where the dendrite joins the soma (S-A distance).", "contents": "Reconstruction of neurons of dorsal horn proper using Golgi-stained serial sections. Three neurons located in the dorsal horn (Lamina III) have been reconstructed using Golgi-stained serial sections. These cells including dendritic and axonal branchings appear to remain within the dorsal horn and to belong to small-sized local interneurons of the dorsal horn proper. The measurements have been made with various criteria: the medio-lateral, dorso-ventral and rostro-caudal extents of the dendritic tree as well as the axonal branchings, total dendritic and axonal length, number of axonal endings and distance from the starting point of the axon at the dendrite to the point where the dendrite joins the soma (S-A distance).", "PMID": 956461}
{"id": "pubmed23n0032_2229", "title": "Trigeminal projections in snakes possessing infrared sensitivity.", "content": "The first order of projections of the trigeminal nerve were studied in four species of snakes, three of which are infrared sensitive, utilizing silver degeneration and cobalt iontophoresis techniques. The trigeminal nerve projected ipsilaterally to a main sensory nucleus, descending spinal nucleus, ventral trigeminal field and solitary nucleus with a contralateral projection to the base of the descending spinal nucleus in all four species. A major additional projection to the lateral nucleus of the trigeminal was present in all three infra-red sensitive species but absent in the other. Iontophoresis of cobalt chloride into a trigeminal branch composed entirely of infrared sensitive fibers and into a trigeminal branch containing no infrared fibers indicated that the lateral nucleus of the trigeminal nerve was the sole first target of the infrared system.", "contents": "Trigeminal projections in snakes possessing infrared sensitivity. The first order of projections of the trigeminal nerve were studied in four species of snakes, three of which are infrared sensitive, utilizing silver degeneration and cobalt iontophoresis techniques. The trigeminal nerve projected ipsilaterally to a main sensory nucleus, descending spinal nucleus, ventral trigeminal field and solitary nucleus with a contralateral projection to the base of the descending spinal nucleus in all four species. A major additional projection to the lateral nucleus of the trigeminal was present in all three infra-red sensitive species but absent in the other. Iontophoresis of cobalt chloride into a trigeminal branch composed entirely of infrared sensitive fibers and into a trigeminal branch containing no infrared fibers indicated that the lateral nucleus of the trigeminal nerve was the sole first target of the infrared system.", "PMID": 956462}
{"id": "pubmed23n0032_2230", "title": "Synapse formation in the mouse olfactory bulb. I. Quantitative studies.", "content": "A quantitative study of synapse formation in the mouse olfactory bulb has been carried out using serial sections. Volumetric synaptic density as well as absolute number of synapses per olfactory bulb for eight distinct synaptic types have been determined at 15 different ages, from the beginning of synapse formation at embryonic day 14 (E14) to postnatal day 44 (P44). Synapses are first found in appreciable numbers at E15 when both axo-dendritic and a few dendro-dendritic synapses occur in the presumptive glomerular layer. Initial synapse formation correlates closely with the reorientation of mitral cells from a primitive tangenital to a definitive radial direction. Synapse formation by mitral cell dendrites occurs after mitral cell axons have grown into the future olfactory cortical areas, either simultaneous with or before synapse formation by these axons. Virtually all synaptic types detected in adults have been found on the day of birth, consistent with the idea that olfaction is an important sensory modality for newborn mice. Volumeric density of a given synaptic type generally increases 50--100 times during development while the absolute number increases about 1,000 times. Synapses in glomeruli develop more precociously than those in the time of origin and differentiation of the principal postsynaptic elements of these two divisions (mitral cells and internal granule cells). Correlation of the time of synapse formation of various synaptic types with their putative excitary or inhibitory role determined in adult studies suggests that excitatory synapses generally form somewhat earlier, although throughout nearly all of synaptic development, both excitatory and inhibitory synapses are present. Reciprocal dendro-dendritic synapses in the external plexiform layer appear to have a special mode of formation. It is suggested that a granule-to-mitral dendro-dendritic synapse only forms next to an already existing mitral-to-granule synapse on the same gemmule.", "contents": "Synapse formation in the mouse olfactory bulb. I. Quantitative studies. A quantitative study of synapse formation in the mouse olfactory bulb has been carried out using serial sections. Volumetric synaptic density as well as absolute number of synapses per olfactory bulb for eight distinct synaptic types have been determined at 15 different ages, from the beginning of synapse formation at embryonic day 14 (E14) to postnatal day 44 (P44). Synapses are first found in appreciable numbers at E15 when both axo-dendritic and a few dendro-dendritic synapses occur in the presumptive glomerular layer. Initial synapse formation correlates closely with the reorientation of mitral cells from a primitive tangenital to a definitive radial direction. Synapse formation by mitral cell dendrites occurs after mitral cell axons have grown into the future olfactory cortical areas, either simultaneous with or before synapse formation by these axons. Virtually all synaptic types detected in adults have been found on the day of birth, consistent with the idea that olfaction is an important sensory modality for newborn mice. Volumeric density of a given synaptic type generally increases 50--100 times during development while the absolute number increases about 1,000 times. Synapses in glomeruli develop more precociously than those in the time of origin and differentiation of the principal postsynaptic elements of these two divisions (mitral cells and internal granule cells). Correlation of the time of synapse formation of various synaptic types with their putative excitary or inhibitory role determined in adult studies suggests that excitatory synapses generally form somewhat earlier, although throughout nearly all of synaptic development, both excitatory and inhibitory synapses are present. Reciprocal dendro-dendritic synapses in the external plexiform layer appear to have a special mode of formation. It is suggested that a granule-to-mitral dendro-dendritic synapse only forms next to an already existing mitral-to-granule synapse on the same gemmule.", "PMID": 956463}
{"id": "pubmed23n0032_2231", "title": "Synapse formation in the mouse olfactory bulb. II. Morphogenesis.", "content": "Serial thin sections of the mouse olfactory bulb from the fourteenth day of gestation (E14) to postnatal to 44 (P44) have been examined in order to study morphogenesis of individual synaptic junctions. At the initiation of synapse formation structures are found that resemble postsynaptic densities but are facing extracellular space or unmodified processes. Transition forms between these isolated postsynaptic densities and undoubted synapses have been found. These observations as well as quantitative studies support the hypothesis that isolated postsynaptic densities can form independently and be converted to synapses when a presynaptic specialization develops opposite them. Detailed studies of olfactory axodendritic synaptogenesis throughout the entire developmental period suggests strongly that these asymmetrical synapses pass through an immature symmetrical phase: (1) symmetrical olfactory axodendritic synapses are found in significantly higher concentration on axonal and dendritic growth cones than on more common processes; (2) the number of symmetrical synapses is correlated with the rate of formation of new synapses determined previously. The time for a recognizable symmetrical synapse to be transformed into a recognizable asymmetrical one has been calculated to be 9--10 hours. A scheme of synapse formation in the CNS has been proposed in which a post-synaptic structure forms independently followed by aggregation of pre-existing presynaptic components into a presynaptic specialization. Different morphogenetic sequences of synapse formation from region to region are attributed simply to different relative rates in the development of the postsynaptic density and the presynaptic specialization.", "contents": "Synapse formation in the mouse olfactory bulb. II. Morphogenesis. Serial thin sections of the mouse olfactory bulb from the fourteenth day of gestation (E14) to postnatal to 44 (P44) have been examined in order to study morphogenesis of individual synaptic junctions. At the initiation of synapse formation structures are found that resemble postsynaptic densities but are facing extracellular space or unmodified processes. Transition forms between these isolated postsynaptic densities and undoubted synapses have been found. These observations as well as quantitative studies support the hypothesis that isolated postsynaptic densities can form independently and be converted to synapses when a presynaptic specialization develops opposite them. Detailed studies of olfactory axodendritic synaptogenesis throughout the entire developmental period suggests strongly that these asymmetrical synapses pass through an immature symmetrical phase: (1) symmetrical olfactory axodendritic synapses are found in significantly higher concentration on axonal and dendritic growth cones than on more common processes; (2) the number of symmetrical synapses is correlated with the rate of formation of new synapses determined previously. The time for a recognizable symmetrical synapse to be transformed into a recognizable asymmetrical one has been calculated to be 9--10 hours. A scheme of synapse formation in the CNS has been proposed in which a post-synaptic structure forms independently followed by aggregation of pre-existing presynaptic components into a presynaptic specialization. Different morphogenetic sequences of synapse formation from region to region are attributed simply to different relative rates in the development of the postsynaptic density and the presynaptic specialization.", "PMID": 956464}
{"id": "pubmed23n0032_2232", "title": "Properties of ganglion cells in the visual streak of the cat's retina.", "content": "The properties of ganglion cells in the visual streak of the cat's retina have been investigated. Evidence is presented that the streak is formed principally, but not entirely, by a concentration of small-bodied ganglion cells with the receptive field properties and slow-conducting axons typical of W-cells.", "contents": "Properties of ganglion cells in the visual streak of the cat's retina. The properties of ganglion cells in the visual streak of the cat's retina have been investigated. Evidence is presented that the streak is formed principally, but not entirely, by a concentration of small-bodied ganglion cells with the receptive field properties and slow-conducting axons typical of W-cells.", "PMID": 956465}
{"id": "pubmed23n0032_2233", "title": "Differential effects of unilateral and bilateral caudate lesions on side preferences and timing behavior in rats.", "content": "Rats were trained to bar press on a differential-reinformcement-of-low-rate 16-sec (DRL 16) schedule for water reinforcement. On alternate days, rats were tested in the presence or absence of a light that signaled the availability of reinforcement. Rats were allowed to bar press on either of two levers (left and right). All rats showed consistent side preferences. For the non-signaled condition but not for the signaled condition, normal baseline rates were related to the strength of side preferences; lower rates and better timing performance were significantly correlated with greater preferences. Unilateral lesions in the caudate nucleus ipsilateral to side preferences facilitated timing performance during nonsignaled test sessions and increased side preferences during both nonsignaled and signaled sessions. Unilateral caudate lesions contralateral to side preferences impaired timing performance during nonsignaled test sessions and decreased side preferences during all sessions. Bilateral caudate lesions transiently depressed response rates without significantly affecting timing performance or side preferences. It is suggested that side preferences are intimately involved in the control of behavior by internal stimuli and that an inherent asymmetry in nigrostriatal function underlies side preferences; the effect of a unilateral striatal lesion will depend upon whether the lesion is placed in the more or less active striatum.", "contents": "Differential effects of unilateral and bilateral caudate lesions on side preferences and timing behavior in rats. Rats were trained to bar press on a differential-reinformcement-of-low-rate 16-sec (DRL 16) schedule for water reinforcement. On alternate days, rats were tested in the presence or absence of a light that signaled the availability of reinforcement. Rats were allowed to bar press on either of two levers (left and right). All rats showed consistent side preferences. For the non-signaled condition but not for the signaled condition, normal baseline rates were related to the strength of side preferences; lower rates and better timing performance were significantly correlated with greater preferences. Unilateral lesions in the caudate nucleus ipsilateral to side preferences facilitated timing performance during nonsignaled test sessions and increased side preferences during both nonsignaled and signaled sessions. Unilateral caudate lesions contralateral to side preferences impaired timing performance during nonsignaled test sessions and decreased side preferences during all sessions. Bilateral caudate lesions transiently depressed response rates without significantly affecting timing performance or side preferences. It is suggested that side preferences are intimately involved in the control of behavior by internal stimuli and that an inherent asymmetry in nigrostriatal function underlies side preferences; the effect of a unilateral striatal lesion will depend upon whether the lesion is placed in the more or less active striatum.", "PMID": 956466}
{"id": "pubmed23n0032_2234", "title": "Evidence against the response-shift account of hyperstriatal function in the pigeon (Columba livia).", "content": "Pigeons with hyperstriatal lesions and unoperated controls were given minimal or extended side-key pretraining prior to acquisition of a position discrimination. Operated birds were impaired following extended, but not minimal, pretraining. The birds then acquired a simultaneous color discrimination with posiversals of the color discrimination, operated birds were impaired, and this was primarily due to an exaggeration of perseverative responding to the former positive stimulus. Analysis of choice latencies found no tendency towards an exaggerated \"Mahut effect\" in hyperstriatals and indicated that operated subjects used the same solution strategies as normals. These findings directly contradict the response-shift account of hyperstriatal function and indicate a return to the response-inhibition hypothesis.", "contents": "Evidence against the response-shift account of hyperstriatal function in the pigeon (Columba livia). Pigeons with hyperstriatal lesions and unoperated controls were given minimal or extended side-key pretraining prior to acquisition of a position discrimination. Operated birds were impaired following extended, but not minimal, pretraining. The birds then acquired a simultaneous color discrimination with posiversals of the color discrimination, operated birds were impaired, and this was primarily due to an exaggeration of perseverative responding to the former positive stimulus. Analysis of choice latencies found no tendency towards an exaggerated \"Mahut effect\" in hyperstriatals and indicated that operated subjects used the same solution strategies as normals. These findings directly contradict the response-shift account of hyperstriatal function and indicate a return to the response-inhibition hypothesis.", "PMID": 956467}
{"id": "pubmed23n0032_2235", "title": "Polarized light discrimination by pigeons and an electroretinographic correlate.", "content": "Pigeons placed in a multiple-key Skinner-box could be trained to choose reliably keys that were aligned in a specific way with the polarization axis of an overhead, randomly rotating light source. On the basis of these results and those of additional control experiments, it is concluded that pigeons can discriminate the axis orientation of linearly polarized light and, furthermore, that they can orient themselves spatially by this cue. Electrophysiological recording experiments showed that the shape of the b-wave of the pigeons' electroretinogram is affected by the axis orientation of linearly polarized flash stimuli. This phenomenon seems to be due to the presence of retinal polarization analyzers that may be tied to color vision mechanisms.", "contents": "Polarized light discrimination by pigeons and an electroretinographic correlate. Pigeons placed in a multiple-key Skinner-box could be trained to choose reliably keys that were aligned in a specific way with the polarization axis of an overhead, randomly rotating light source. On the basis of these results and those of additional control experiments, it is concluded that pigeons can discriminate the axis orientation of linearly polarized light and, furthermore, that they can orient themselves spatially by this cue. Electrophysiological recording experiments showed that the shape of the b-wave of the pigeons' electroretinogram is affected by the axis orientation of linearly polarized flash stimuli. This phenomenon seems to be due to the presence of retinal polarization analyzers that may be tied to color vision mechanisms.", "PMID": 956468}
{"id": "pubmed23n0032_2236", "title": "Experiential and hormonal conditions affect squab-egg choice in ring doves (Streptopelia risoria).", "content": "Noncycling, progesterone-injected, experienced male ring doves prefer eggs to squabs in a simultaneous squab-egg choice test, while experienced male doves injected with both progesterone and prolactin prefer squabs to eggs. When exogenous prolactin is administered to either naive or experienced male ring doves during the early phase of a normal incubation cycle, squab choice is significantly increased. Squab choice is also significantly increased in incubating doves by 24 hr of exposure to a squab in the home nest. This effect is greater in naive than in experienced doves. Squabs introduced during late incubation have more of a positive effect on squab choice than when introduced during early incubation. It is concluded that intracyclic experience organizes successive behavioral changes within a reproductive cycle and that naive doves are more affected by some changes in intracyclic events than are experienced doves.", "contents": "Experiential and hormonal conditions affect squab-egg choice in ring doves (Streptopelia risoria). Noncycling, progesterone-injected, experienced male ring doves prefer eggs to squabs in a simultaneous squab-egg choice test, while experienced male doves injected with both progesterone and prolactin prefer squabs to eggs. When exogenous prolactin is administered to either naive or experienced male ring doves during the early phase of a normal incubation cycle, squab choice is significantly increased. Squab choice is also significantly increased in incubating doves by 24 hr of exposure to a squab in the home nest. This effect is greater in naive than in experienced doves. Squabs introduced during late incubation have more of a positive effect on squab choice than when introduced during early incubation. It is concluded that intracyclic experience organizes successive behavioral changes within a reproductive cycle and that naive doves are more affected by some changes in intracyclic events than are experienced doves.", "PMID": 956469}
{"id": "pubmed23n0032_2237", "title": "Facilitation of receptivity in estrogen-primed rats during successive mating tests with progestins and methysergide.", "content": "The role of hormonal and experiential variables in mediating the gradual increase of receptivity occurring during weekly tests was evaluated in adult ovariectomized females. Hormone treatment consisted of estradiol benzoate (EB) injection followed by progesterone (P), 20alpha-hydroxyprogesterone (20alpha-OHP), methysergide maleate (Me), or oil during weekly mating tests. The experiential conditions were vaginal stimulation, mounts only, or no interaction with males. There was a gradual increase in receptivity in all groups even en the one receiving oil. Progressive facilitation occurred even when no interaction with the male was permitted. Interchanging the synergists on the sixth and seventh tests was not detrimental to the display of receptivity attained with synergists given previously. Thus, we concluded that successive injections of EB rather than synergists or experience mediate this increase in receptivity.", "contents": "Facilitation of receptivity in estrogen-primed rats during successive mating tests with progestins and methysergide. The role of hormonal and experiential variables in mediating the gradual increase of receptivity occurring during weekly tests was evaluated in adult ovariectomized females. Hormone treatment consisted of estradiol benzoate (EB) injection followed by progesterone (P), 20alpha-hydroxyprogesterone (20alpha-OHP), methysergide maleate (Me), or oil during weekly mating tests. The experiential conditions were vaginal stimulation, mounts only, or no interaction with males. There was a gradual increase in receptivity in all groups even en the one receiving oil. Progressive facilitation occurred even when no interaction with the male was permitted. Interchanging the synergists on the sixth and seventh tests was not detrimental to the display of receptivity attained with synergists given previously. Thus, we concluded that successive injections of EB rather than synergists or experience mediate this increase in receptivity.", "PMID": 956470}
{"id": "pubmed23n0032_2238", "title": "Carotene and immunoglobulin concentrations in the colostrum and milk of pasture-fed cows.", "content": "The carotene and immunoglobulin concentrations in colostra and milks of 6 Jersey, 4 Friesian and 6 Jersey x Friesian cows were determined during the first 21 d of lactation. Carotene concentrations of between 50 and 300ug/g milk fat were found at first milking and these declined to normal concentrations, in the range 9-21ug/g milk fat, by the eighth to tenth day of lactation. Immunoglobulin concentrations at first milking were in the range 4-40g/l milk and decreased to normal concentrations, in the range 0.3-0.5g/l, by the fifth to seventh day of lactation. For both substances the data from each cow's milk fitted an exponential decay model. The fractional rates of decrease in concentration of carotene were 0.19-0.57/d and of immunoglobulin were 0.40-0.73/d. Breed-group differences were observed in both carotene and immunoglobulin concentrations in milks after the fourteenth day of lactation. In Jersey milks, the average carotene and immunoglobulin concentrations were respectively 18.5 ug/g milk fat and 0.32g/l of milk, while in Friesian milks the concentrations were respectively 11.4 ug/g milk fat and 0.46 g/l milk. There was also a difference between breed groups in the daily rates of decrease of the immunoglobulin concentrations. The average rate of decrease for the Friesians was about 1.5 times the rate for the Jerseys.", "contents": "Carotene and immunoglobulin concentrations in the colostrum and milk of pasture-fed cows. The carotene and immunoglobulin concentrations in colostra and milks of 6 Jersey, 4 Friesian and 6 Jersey x Friesian cows were determined during the first 21 d of lactation. Carotene concentrations of between 50 and 300ug/g milk fat were found at first milking and these declined to normal concentrations, in the range 9-21ug/g milk fat, by the eighth to tenth day of lactation. Immunoglobulin concentrations at first milking were in the range 4-40g/l milk and decreased to normal concentrations, in the range 0.3-0.5g/l, by the fifth to seventh day of lactation. For both substances the data from each cow's milk fitted an exponential decay model. The fractional rates of decrease in concentration of carotene were 0.19-0.57/d and of immunoglobulin were 0.40-0.73/d. Breed-group differences were observed in both carotene and immunoglobulin concentrations in milks after the fourteenth day of lactation. In Jersey milks, the average carotene and immunoglobulin concentrations were respectively 18.5 ug/g milk fat and 0.32g/l of milk, while in Friesian milks the concentrations were respectively 11.4 ug/g milk fat and 0.46 g/l milk. There was also a difference between breed groups in the daily rates of decrease of the immunoglobulin concentrations. The average rate of decrease for the Friesians was about 1.5 times the rate for the Jerseys.", "PMID": 956471}
{"id": "pubmed23n0032_2239", "title": "Autoxidation in milk rich in linoleic acid. II. Modification of the initiation system and control of oxidation.", "content": "Factors contributing to the initiation of lipid oxidation in cow's and mare's milk containing high levels of polyunsaturated fatty acids were studied. Addition of H2O2 just after milking, in slight excess of the stoichiometric amounts required to destroy ascorbic acid, delayed the development of oxidized flavours in cow's milk high in linoleic acid. Hydrogen peroxide treatment followed by the addition of alpha-or gamma-tocopherols prevented lipid oxidation in cow's milk even when 0.1 mg Cu/l milk was added. When used separately in the presence of Cu these treatments were ineffective as was butylated hydroxyanisole treatment. The lipid and ascorbic acid in mare's milk were remarkably stable to oxidation. Addition of 0.05 or 0.1 mg Cu/l, ethylenediamine tetraacetic acid, neocuproine, or H2O2 had very little effect on the loss of ascorbic acid and lipid oxidation in mare's milk.", "contents": "Autoxidation in milk rich in linoleic acid. II. Modification of the initiation system and control of oxidation. Factors contributing to the initiation of lipid oxidation in cow's and mare's milk containing high levels of polyunsaturated fatty acids were studied. Addition of H2O2 just after milking, in slight excess of the stoichiometric amounts required to destroy ascorbic acid, delayed the development of oxidized flavours in cow's milk high in linoleic acid. Hydrogen peroxide treatment followed by the addition of alpha-or gamma-tocopherols prevented lipid oxidation in cow's milk even when 0.1 mg Cu/l milk was added. When used separately in the presence of Cu these treatments were ineffective as was butylated hydroxyanisole treatment. The lipid and ascorbic acid in mare's milk were remarkably stable to oxidation. Addition of 0.05 or 0.1 mg Cu/l, ethylenediamine tetraacetic acid, neocuproine, or H2O2 had very little effect on the loss of ascorbic acid and lipid oxidation in mare's milk.", "PMID": 956472}
{"id": "pubmed23n0032_2240", "title": "Enzymic methods for estimation of the somatic cell count in bovine milk. 1. Development of assay techniques and a study of their usefulness in evaluating the somatic cell content of milk.", "content": "Assay procedures were developed for a number of enzymes in milk which apparently originate from leucocytes. The enzymes studied were acid phosphatase, N-acetyl-beta-D-glucosaminidase, beta-glucuronidase, arylsulphatase, alpha-mannosidase, and catalase. Quarter-milk samples were analysed for enzyme activity and results compared with the electronic cell count and the Wisconsin Mastitis Test. All enzymes measured except acid phosphatase and alpha-mannosidase showed good correlation with the electronic cell count. Of the other 4 enzymes tested, beta-glucuronidase and arylsulphatase were unsuitable as diagnostic aids owing to the lengthy incubation periods required in their assay procedures. The assay of catalase, which involved the measurement of the initial rate of release of O2 using an O2 analyser apparatus, was rapid, sensitive and reasonably reliable, if fresh milk samples were used. The assay procedure for N-acetyl-beta-D-glucosaminidase was considered to be the most reliable, simple and rapid enzymic method for estimating the number of somatic cells in milk.", "contents": "Enzymic methods for estimation of the somatic cell count in bovine milk. 1. Development of assay techniques and a study of their usefulness in evaluating the somatic cell content of milk. Assay procedures were developed for a number of enzymes in milk which apparently originate from leucocytes. The enzymes studied were acid phosphatase, N-acetyl-beta-D-glucosaminidase, beta-glucuronidase, arylsulphatase, alpha-mannosidase, and catalase. Quarter-milk samples were analysed for enzyme activity and results compared with the electronic cell count and the Wisconsin Mastitis Test. All enzymes measured except acid phosphatase and alpha-mannosidase showed good correlation with the electronic cell count. Of the other 4 enzymes tested, beta-glucuronidase and arylsulphatase were unsuitable as diagnostic aids owing to the lengthy incubation periods required in their assay procedures. The assay of catalase, which involved the measurement of the initial rate of release of O2 using an O2 analyser apparatus, was rapid, sensitive and reasonably reliable, if fresh milk samples were used. The assay procedure for N-acetyl-beta-D-glucosaminidase was considered to be the most reliable, simple and rapid enzymic method for estimating the number of somatic cells in milk.", "PMID": 956473}
{"id": "pubmed23n0032_2241", "title": "Autolysis of Streptococcus cremoris.", "content": "The autolysin of Streptococcus cremoris had the specificity of an endo-N-acetylmuramidase as it hydrolysed the linkage between N-acetylmuramic acid and N-acetylglucosamine. The enzyme had no amidase or endopeptidase action. It reached highest activity in the exponential phase of growth and in the electron microscope seemed to fragment the coccal wall at the equatorial ring.", "contents": "Autolysis of Streptococcus cremoris. The autolysin of Streptococcus cremoris had the specificity of an endo-N-acetylmuramidase as it hydrolysed the linkage between N-acetylmuramic acid and N-acetylglucosamine. The enzyme had no amidase or endopeptidase action. It reached highest activity in the exponential phase of growth and in the electron microscope seemed to fragment the coccal wall at the equatorial ring.", "PMID": 956474}
{"id": "pubmed23n0032_2242", "title": "Factors that influence milk cholesterol and lipid phosphorus: content and distribution.", "content": "Average cholesterol content of 356 raw milk samples was 152.2 mug/ml and upon centrifugation (3000 X g for 8 min), 16.9% was distributed in the skim milk phase. Lipid phosphorus averaged 19.0 mug/ml and was partitioned 50:50 between cream and skim milk phases. Weight ratios of cholesterol to lipid phosphorus for milk and skim milk were 8.30:1 and 2.94:1. When variation due to milk yield, fat percent, and somatic cell numbers (deoxyribonucleic acid percent reflectance) was accounted for by least squares, cholesterol content and distribution did not differ among breeds (Holstein, Jersey, and Guernsey). Breed differences in lipid phosphorus content of whole milk could not be detected. However, Holsteins had a significantly lower content of lipid phosphorus and a higher weight ratio for skim milk. Milk yield, fat content, and somatic cells affected responses of cholesterol and lipid phosphorus. This supports a multiple origin concept for membrane material in skim milk.", "contents": "Factors that influence milk cholesterol and lipid phosphorus: content and distribution. Average cholesterol content of 356 raw milk samples was 152.2 mug/ml and upon centrifugation (3000 X g for 8 min), 16.9% was distributed in the skim milk phase. Lipid phosphorus averaged 19.0 mug/ml and was partitioned 50:50 between cream and skim milk phases. Weight ratios of cholesterol to lipid phosphorus for milk and skim milk were 8.30:1 and 2.94:1. When variation due to milk yield, fat percent, and somatic cell numbers (deoxyribonucleic acid percent reflectance) was accounted for by least squares, cholesterol content and distribution did not differ among breeds (Holstein, Jersey, and Guernsey). Breed differences in lipid phosphorus content of whole milk could not be detected. However, Holsteins had a significantly lower content of lipid phosphorus and a higher weight ratio for skim milk. Milk yield, fat content, and somatic cells affected responses of cholesterol and lipid phosphorus. This supports a multiple origin concept for membrane material in skim milk.", "PMID": 956477}
{"id": "pubmed23n0032_2243", "title": "Adenosine triphosphate in milk.", "content": "Freshly secreted goat's milk contains a number of viable metabolic pathways including those for the synthesis of triglycerides and phospholipids. Toward understanding this matter, amounts of the fundamentally important energy substrate, adenosine triphosphate, in goat's milk were evaluated. Milk left in the goat udder overnight had less adenosine triphosphate (12.4 muM) than fresh secreted milk (37.6 muM). In similar experiments the skim milk derived from whole accumulating in the udder overnight was lower in adenosine triphosphate (14.2 muM) than skim milk from freshly secreted milk (26.0 muM). To determine changes in quantities of adenosine triphosphate after milking, milks were divided into two parts, one containing only milk and the other milk plus 2,4-dinitrophenol and sodium arsenate, inhibitors of oxidative and substrate level phosphorylation. Adenosine triphosphate in milk decreased during 4-h in vitro incubations, and the rate of decline was markedly greater in the presence of the inhibitors. Thus, freshly secreted goat's milk contains significant amounts of adenosine triphosphate, and more can be synthesized therein after removal from the udder. In limited samples human and bovine milks contained much lower concentrations of the compound than those in goat's milk.", "contents": "Adenosine triphosphate in milk. Freshly secreted goat's milk contains a number of viable metabolic pathways including those for the synthesis of triglycerides and phospholipids. Toward understanding this matter, amounts of the fundamentally important energy substrate, adenosine triphosphate, in goat's milk were evaluated. Milk left in the goat udder overnight had less adenosine triphosphate (12.4 muM) than fresh secreted milk (37.6 muM). In similar experiments the skim milk derived from whole accumulating in the udder overnight was lower in adenosine triphosphate (14.2 muM) than skim milk from freshly secreted milk (26.0 muM). To determine changes in quantities of adenosine triphosphate after milking, milks were divided into two parts, one containing only milk and the other milk plus 2,4-dinitrophenol and sodium arsenate, inhibitors of oxidative and substrate level phosphorylation. Adenosine triphosphate in milk decreased during 4-h in vitro incubations, and the rate of decline was markedly greater in the presence of the inhibitors. Thus, freshly secreted goat's milk contains significant amounts of adenosine triphosphate, and more can be synthesized therein after removal from the udder. In limited samples human and bovine milks contained much lower concentrations of the compound than those in goat's milk.", "PMID": 956478}
{"id": "pubmed23n0032_2244", "title": "Relative activation of milk lipoprotein lipase by serum of cows fed varying amounts of fat.", "content": "A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag ime of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the assay. Inhibition of activation was indicated at high amounts (10%) of serum. Activation from plasma was half that from serum, presumably caused by an increase in substrate Km in the presence of heparin. Use of glyceryl tri[9,10-3H] oleate yielded excessively high blanks; [2-3H] glyceryl triolein is suggested for routine assay. Relative amounts of activator were not different between dry and lactating cows fed \"conventional\" diets. Activator concentration increased linearly with increasing dietary fat and was related to concentration of total lipid in plasma. The assay may provide a useful adjunct in studies on lipoprotein metabolism.", "contents": "Relative activation of milk lipoprotein lipase by serum of cows fed varying amounts of fat. A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag ime of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the assay. Inhibition of activation was indicated at high amounts (10%) of serum. Activation from plasma was half that from serum, presumably caused by an increase in substrate Km in the presence of heparin. Use of glyceryl tri[9,10-3H] oleate yielded excessively high blanks; [2-3H] glyceryl triolein is suggested for routine assay. Relative amounts of activator were not different between dry and lactating cows fed \"conventional\" diets. Activator concentration increased linearly with increasing dietary fat and was related to concentration of total lipid in plasma. The assay may provide a useful adjunct in studies on lipoprotein metabolism.", "PMID": 956479}
{"id": "pubmed23n0032_2245", "title": "Mechanisms of secretion: effects of colchicine and vincristine on composition and flow of milk in the goat.", "content": "Colchicine, the plant alkaloid, produced a dramatic decrease in milk flow when infused into the udder of the goat. The compound (1 to 5 mg) dissolved in 5 ml of water was inserted into one side of the under via the teat canal. Such treatments consistently caused a depression in milk yield from the infused side with maximum at 36 h and substantial reversal by 72 to 96 h. Milks from both the infused and uninfused sides of the udder were essentially normal in composition (fat, protein, and lactose). However, globulins and riboflavin were elevated in milks from the infused side. The plant alkaloid, vincristine, produced effects on milk secretion similar to those of colchicine but at dosages roughly one-tenth the latter. The two substances had no effect on the amount of milk from the uninfused side of the udder. Experiments employing [carbon-14] colchicine revealed that less than 20% of the infused colchicine is secreted in the milk. Both the secretion of fat globules and the emptying of secretory vesicles by the lactating cell are inhibited by colchicine indicating that a portion of the cell population is turned off from secretion. Plant substances such as colchicine and vincristine may at times limit yields of milk, especially in grazing ruminants.", "contents": "Mechanisms of secretion: effects of colchicine and vincristine on composition and flow of milk in the goat. Colchicine, the plant alkaloid, produced a dramatic decrease in milk flow when infused into the udder of the goat. The compound (1 to 5 mg) dissolved in 5 ml of water was inserted into one side of the under via the teat canal. Such treatments consistently caused a depression in milk yield from the infused side with maximum at 36 h and substantial reversal by 72 to 96 h. Milks from both the infused and uninfused sides of the udder were essentially normal in composition (fat, protein, and lactose). However, globulins and riboflavin were elevated in milks from the infused side. The plant alkaloid, vincristine, produced effects on milk secretion similar to those of colchicine but at dosages roughly one-tenth the latter. The two substances had no effect on the amount of milk from the uninfused side of the udder. Experiments employing [carbon-14] colchicine revealed that less than 20% of the infused colchicine is secreted in the milk. Both the secretion of fat globules and the emptying of secretory vesicles by the lactating cell are inhibited by colchicine indicating that a portion of the cell population is turned off from secretion. Plant substances such as colchicine and vincristine may at times limit yields of milk, especially in grazing ruminants.", "PMID": 956480}
{"id": "pubmed23n0032_2246", "title": "Distribution and clearance of polybrominated biphenyls in cows and calves.", "content": "The distribution and clearance of polybrominated biphenyls were determined in two cows and a calf fed polybrominated biphenyls by boluses, by placental transport to a calf from a contaminated dam, and to another calf via milk from the contaminated dam. Feces were an important route of excretion of polybrominated biphenyls during exposure and the period when the contaminated feed was cleared from the digestive tract. Approximately 50% of single intraruminal doses of these hydrocarbons were cleared by 168 h. Free polybrominated biphenyls in urine could not be quantitated. However, 24% of carbon-14 from tetrachlorobiphenyl was recovered, suggesting that significant quantities of halogenated biphenyl may be excreted in urine. Milk was also an important excretion route with one cow excreting 23% of the polybrominated biphenyl dose in 168 days. Concentrations of polybrominated biphenyls in the fat of most tissues were similar, with higher concentrations in liver and lower in the nervous system. Gross or histopathologic lesions of tissues collected at necropsy could not be related specifically to polybrominated biphenyl toxicity.", "contents": "Distribution and clearance of polybrominated biphenyls in cows and calves. The distribution and clearance of polybrominated biphenyls were determined in two cows and a calf fed polybrominated biphenyls by boluses, by placental transport to a calf from a contaminated dam, and to another calf via milk from the contaminated dam. Feces were an important route of excretion of polybrominated biphenyls during exposure and the period when the contaminated feed was cleared from the digestive tract. Approximately 50% of single intraruminal doses of these hydrocarbons were cleared by 168 h. Free polybrominated biphenyls in urine could not be quantitated. However, 24% of carbon-14 from tetrachlorobiphenyl was recovered, suggesting that significant quantities of halogenated biphenyl may be excreted in urine. Milk was also an important excretion route with one cow excreting 23% of the polybrominated biphenyl dose in 168 days. Concentrations of polybrominated biphenyls in the fat of most tissues were similar, with higher concentrations in liver and lower in the nervous system. Gross or histopathologic lesions of tissues collected at necropsy could not be related specifically to polybrominated biphenyl toxicity.", "PMID": 956481}
{"id": "pubmed23n0032_2247", "title": "Excretion of levamisole in milk from cows treated with various formulations.", "content": "The rate of disappearance of levamisole in milk from cows given levamisole hydrochloride drench, levamisole resinate in feed pellets, levamisole hydrochloride boluses, or levamisole phosphate injectable was determined. Each formulation was given as a single treatment to each of five cows at a rate equivalent to 8 mg of levamisole hydrochloride/kg of body weight. Levamisole hydrochloride residues in milk averaged .50, .55, .58, and .32 ppm at 12 hr after the administration of levamisole drench, feed, bolus, and injectable formulations. Levamisole hydrochloride residues were below .01 ppm in milk at 48 h after drench treatment and at 60 h after treatment with other three formulations. Toxicity symptoms were not observed in any cows following treatment.", "contents": "Excretion of levamisole in milk from cows treated with various formulations. The rate of disappearance of levamisole in milk from cows given levamisole hydrochloride drench, levamisole resinate in feed pellets, levamisole hydrochloride boluses, or levamisole phosphate injectable was determined. Each formulation was given as a single treatment to each of five cows at a rate equivalent to 8 mg of levamisole hydrochloride/kg of body weight. Levamisole hydrochloride residues in milk averaged .50, .55, .58, and .32 ppm at 12 hr after the administration of levamisole drench, feed, bolus, and injectable formulations. Levamisole hydrochloride residues were below .01 ppm in milk at 48 h after drench treatment and at 60 h after treatment with other three formulations. Toxicity symptoms were not observed in any cows following treatment.", "PMID": 956482}
{"id": "pubmed23n0032_2248", "title": "Factors influencing rumen fermentation: effect of hydrogen on formation of propionate.", "content": "The effect of hydrogen on fermentation of lactate, pyruvate, fumarate, and succinate by resting rumen microorganisms has been investigated. Under an atmosphere of nitrogen, lactate was fermented to yield acetate as the major product (85 to 100 mole %) and propionate (0 to 17 mole %) and butyrate (0 to 3%) as secondary products. Under hydrogen, there was increased formation of both propionate and total volatile fatty acids. The amount of propionate increased 4 to 8 times and total volatile fatty acids 2.5 to 3.2 times. Propionate formation was proportional to the hydrogen concentration and reached a maximum at a partial pressure of hydrogen of .2 N/m2. With [2-carbon-14] lactate, propionate was formed via the dicarboxylic acid pathway under both nitrogen or hydrogen. Hydrogen did not affect significantly the fermentation of pyruvate or succinate. With fumarate under hydrogen, propionate and total volatile fatty acids increased 6.8 and 2 times while acetate was unchanged. The mechanism by which hydrogen exerts these effects is discussed in relation to the role of methanogenesis in the rumen.", "contents": "Factors influencing rumen fermentation: effect of hydrogen on formation of propionate. The effect of hydrogen on fermentation of lactate, pyruvate, fumarate, and succinate by resting rumen microorganisms has been investigated. Under an atmosphere of nitrogen, lactate was fermented to yield acetate as the major product (85 to 100 mole %) and propionate (0 to 17 mole %) and butyrate (0 to 3%) as secondary products. Under hydrogen, there was increased formation of both propionate and total volatile fatty acids. The amount of propionate increased 4 to 8 times and total volatile fatty acids 2.5 to 3.2 times. Propionate formation was proportional to the hydrogen concentration and reached a maximum at a partial pressure of hydrogen of .2 N/m2. With [2-carbon-14] lactate, propionate was formed via the dicarboxylic acid pathway under both nitrogen or hydrogen. Hydrogen did not affect significantly the fermentation of pyruvate or succinate. With fumarate under hydrogen, propionate and total volatile fatty acids increased 6.8 and 2 times while acetate was unchanged. The mechanism by which hydrogen exerts these effects is discussed in relation to the role of methanogenesis in the rumen.", "PMID": 956483}
{"id": "pubmed23n0032_2249", "title": "Coffee grounds. I. Effects of coffee grounds on ration digestibility and diuresis in cattle, on in vitro rumen fermentation, and on rat growth.", "content": "Holstein steers were used to compare rations in which coffee grounds replaced grain at rates of 0, 5, 10, or 20%. The coffee grounds analyzed (%) dry matter 91.1, protein 11.8, fat 23.1, fiber 42.5, ash .7, and caffeine .13. Digestibility coefficients for dry matter, crude protein, and energy decreased, and ether extract increased progressively as concentrations of coffee grounds in the ration were increased. There was a significant decrease in nitrogen retention and utilization when the rations contained 10 or 20% coffee grounds. Coffee grounds depressed feed intake, increased urinary output, and increased water intake (based on dry matter intake). Coffee grounds caused diuresis and renal, urethral, and bladder irritation. Adding coffee grounds up to 35% of the substrate did not depress rumen microbial fermentation, as measured by in vitro gas production. Weight gain of rats fed rations containing 22.5% untreated coffee grounds was reduced significantly. Water extraction removed some undersirable factors in coffee grounds for rats, and ether extraction removed most of them. Incremental additions of coffee oil (1, 3, or 5%) to the basal diet resulted in incremental decreases in rat weight gain.", "contents": "Coffee grounds. I. Effects of coffee grounds on ration digestibility and diuresis in cattle, on in vitro rumen fermentation, and on rat growth. Holstein steers were used to compare rations in which coffee grounds replaced grain at rates of 0, 5, 10, or 20%. The coffee grounds analyzed (%) dry matter 91.1, protein 11.8, fat 23.1, fiber 42.5, ash .7, and caffeine .13. Digestibility coefficients for dry matter, crude protein, and energy decreased, and ether extract increased progressively as concentrations of coffee grounds in the ration were increased. There was a significant decrease in nitrogen retention and utilization when the rations contained 10 or 20% coffee grounds. Coffee grounds depressed feed intake, increased urinary output, and increased water intake (based on dry matter intake). Coffee grounds caused diuresis and renal, urethral, and bladder irritation. Adding coffee grounds up to 35% of the substrate did not depress rumen microbial fermentation, as measured by in vitro gas production. Weight gain of rats fed rations containing 22.5% untreated coffee grounds was reduced significantly. Water extraction removed some undersirable factors in coffee grounds for rats, and ether extraction removed most of them. Incremental additions of coffee oil (1, 3, or 5%) to the basal diet resulted in incremental decreases in rat weight gain.", "PMID": 956484}
{"id": "pubmed23n0032_2250", "title": "Corn grain preserved with ammonium isobutyrate for lactating dairy cattle.", "content": "Twenty-four lactating cows were fed one of three complete rations containing dry corn, ensiled corn, or corn treated with ammonium isobutyrate in a Latin square arrangement of treatments. Two weeks were used for adjustments to rations and 3 wk for collection of data. There were no differences in average daily yield of milk or fat-corrected milk, but fat percentage of milk from cows fed ensiled corn was lower than that of those given dry corn. Fat test of cows fed treated corn was intermediate and not different from the other groups. Molar proportions of volatile fatty acids and concentrations of rumen ammonia were not different among treatments.", "contents": "Corn grain preserved with ammonium isobutyrate for lactating dairy cattle. Twenty-four lactating cows were fed one of three complete rations containing dry corn, ensiled corn, or corn treated with ammonium isobutyrate in a Latin square arrangement of treatments. Two weeks were used for adjustments to rations and 3 wk for collection of data. There were no differences in average daily yield of milk or fat-corrected milk, but fat percentage of milk from cows fed ensiled corn was lower than that of those given dry corn. Fat test of cows fed treated corn was intermediate and not different from the other groups. Molar proportions of volatile fatty acids and concentrations of rumen ammonia were not different among treatments.", "PMID": 956485}
{"id": "pubmed23n0032_2251", "title": "Responses of young calves to low doses of lead.", "content": "Twenty calves, week-old male Holsteins, 42.8 kg body weight, were assigned to four treatments by oral dose 0, 1.5, 3.0, and 6.0 mg lead carbonate/kg body weight given three times weekly until calf fetal hemoglobin had been converted to 100% adult hemoglobin for 2 consecutive wk. Analyses were for the first 7 wk when data were complete. Variables included lead in blood, body weight changes, erythrocyte delta-aminolevulinic acid dehydrase activity, packed cell volume, hemoglobin, conversion of fetal to adult hemoglobin, and erythrocyte 2,3-diphosphoglyceric acid. Early changes due to lead treatment were inhibition of delta-aminolevulinic acid dehydrase at all doses and increased loss of hemoglobin at 3.0 and 6.0 mg doses. Growth rate was not impaired seriously by lead treatment. The 2,3-diphosphoglyceric acid in erythrocytes reached peak value at 3 to 4 wk of age and then decreased with age, but 2,3-diphosphoglyceric acid of the treated calves was not changed by the low lead dosing. Low lead dosing will induce early metabolic changes in the young calf prior to inhibition of growth.", "contents": "Responses of young calves to low doses of lead. Twenty calves, week-old male Holsteins, 42.8 kg body weight, were assigned to four treatments by oral dose 0, 1.5, 3.0, and 6.0 mg lead carbonate/kg body weight given three times weekly until calf fetal hemoglobin had been converted to 100% adult hemoglobin for 2 consecutive wk. Analyses were for the first 7 wk when data were complete. Variables included lead in blood, body weight changes, erythrocyte delta-aminolevulinic acid dehydrase activity, packed cell volume, hemoglobin, conversion of fetal to adult hemoglobin, and erythrocyte 2,3-diphosphoglyceric acid. Early changes due to lead treatment were inhibition of delta-aminolevulinic acid dehydrase at all doses and increased loss of hemoglobin at 3.0 and 6.0 mg doses. Growth rate was not impaired seriously by lead treatment. The 2,3-diphosphoglyceric acid in erythrocytes reached peak value at 3 to 4 wk of age and then decreased with age, but 2,3-diphosphoglyceric acid of the treated calves was not changed by the low lead dosing. Low lead dosing will induce early metabolic changes in the young calf prior to inhibition of growth.", "PMID": 956486}
{"id": "pubmed23n0032_2252", "title": "Characterization of fatty acid synthesis by cow mammary subcellular fractions.", "content": "Cellular site of fatty acid synthesis was investigated with mammary tissue from lactating cows. Cytosol, mitochondrial, and microsomal fractions were obtained by differential centrifugation and characterized by measurement of marker enzymes. Two incubation media were utilized to quantitate acetate incorporation into fatty acids by the subcellular fractions. The cytosol fraction synthesized fatty acids from 4 to 16-carbons in chain length with the pattern similar to those synthesized in vivo. No significant acetate incorporation into fatty acids was obtained with mitochondria or microsomal incubations. The malonyl-CoA pathway was the predominant pathway of fatty acid synthesis in cow mammary cytosol as evidenced by inhibitory studies with avidin.", "contents": "Characterization of fatty acid synthesis by cow mammary subcellular fractions. Cellular site of fatty acid synthesis was investigated with mammary tissue from lactating cows. Cytosol, mitochondrial, and microsomal fractions were obtained by differential centrifugation and characterized by measurement of marker enzymes. Two incubation media were utilized to quantitate acetate incorporation into fatty acids by the subcellular fractions. The cytosol fraction synthesized fatty acids from 4 to 16-carbons in chain length with the pattern similar to those synthesized in vivo. No significant acetate incorporation into fatty acids was obtained with mitochondria or microsomal incubations. The malonyl-CoA pathway was the predominant pathway of fatty acid synthesis in cow mammary cytosol as evidenced by inhibitory studies with avidin.", "PMID": 956487}
{"id": "pubmed23n0032_2253", "title": "Relation between mammary gland development and body weight.", "content": "Mammary gland indices including deoxyribonucleic  acid, dry fat-free tissue, and wet weight were correlated with body weight in species having a range of 20,000 fold in body weight. Included were mice, hamsters, rats, guinea pigs, rabbits, sheep, and cattle. From limited data accumulated at Missouri, the relation of deoxyribonucleic acid of mammary gland (Y, mg) to bodyweight (X, g) was Y = .53X-79 at the peak of lactation, Y = .29X-82 at the end of pregnancy, and Y = .056X-84 at the beginning of pregnancy. Comparing mammary dry fat-free tissue (mg) to body weight resulted in: Y = 20X-80, Y = 9.7X-85, and Y = 2.6X-91 in the same order while equations for mammary gland wet weight (mg) and body weight were: Y = 135X-82, Y = 123X-79, and Y = 38X-85. The composite value for regression (b) when all data were combined was .80. These mammary indices relate to but are slightly higher than metabolic body weight which is accepted generally as body weight to the three-quarter power.", "contents": "Relation between mammary gland development and body weight. Mammary gland indices including deoxyribonucleic  acid, dry fat-free tissue, and wet weight were correlated with body weight in species having a range of 20,000 fold in body weight. Included were mice, hamsters, rats, guinea pigs, rabbits, sheep, and cattle. From limited data accumulated at Missouri, the relation of deoxyribonucleic acid of mammary gland (Y, mg) to bodyweight (X, g) was Y = .53X-79 at the peak of lactation, Y = .29X-82 at the end of pregnancy, and Y = .056X-84 at the beginning of pregnancy. Comparing mammary dry fat-free tissue (mg) to body weight resulted in: Y = 20X-80, Y = 9.7X-85, and Y = 2.6X-91 in the same order while equations for mammary gland wet weight (mg) and body weight were: Y = 135X-82, Y = 123X-79, and Y = 38X-85. The composite value for regression (b) when all data were combined was .80. These mammary indices relate to but are slightly higher than metabolic body weight which is accepted generally as body weight to the three-quarter power.", "PMID": 956488}
{"id": "pubmed23n0032_2254", "title": "Measurement of 25-hydroxycholecalciferol in the bovine.", "content": "Bovine plasma 25-hydroxycholecalciferol was estimated by rapid protein binding assay. Serum-binding proteins in the assay were from rats deficient in vitamin D and from normal rats; no differences existed in standard curves. Nanograms 25-hydroxycholecalciferol in eight cows averaged 45.5 +/- 12.2 ml of plasma. Results from the rapid protein binding assay compared favorably (greater than 10% deviation) with values from a 25-hydroxycholecalciferol assay involving high pressure liquid chromatography.", "contents": "Measurement of 25-hydroxycholecalciferol in the bovine. Bovine plasma 25-hydroxycholecalciferol was estimated by rapid protein binding assay. Serum-binding proteins in the assay were from rats deficient in vitamin D and from normal rats; no differences existed in standard curves. Nanograms 25-hydroxycholecalciferol in eight cows averaged 45.5 +/- 12.2 ml of plasma. Results from the rapid protein binding assay compared favorably (greater than 10% deviation) with values from a 25-hydroxycholecalciferol assay involving high pressure liquid chromatography.", "PMID": 956489}
{"id": "pubmed23n0032_2255", "title": "Prenancy diagnosis in dairy cattle by progesterone concentration in milk.", "content": "Concentration of progesterone in whole milk was used to diagnose pregnancy in lactating dairy cows. Milk samples were taken 21 to 22 days postbreeding and analyzed for progesterone by radioimmunoassay. Progesterone in milk greater than 11 ng/ml, 8 to 11 ng/ml, and less than 8 ng/ml was used classify animals as pregnant, questionable, and not pregnant. In this classification for 123 cows, 67 were classified pregnant, 7 questionable, and 49 not pregnant. A comparison of progesterone diagnosis with actual calving results showed agreement of 73 and 98% for the cows classified as pregnant and not pregnant. Thirteen of 19 animals, in which there were discrepancies between diagnoses by milk progesterone and calving results, were not detected in estrus within 30 days after breeding. One animal classified not pregnant by milk progesterone was pregnant.", "contents": "Prenancy diagnosis in dairy cattle by progesterone concentration in milk. Concentration of progesterone in whole milk was used to diagnose pregnancy in lactating dairy cows. Milk samples were taken 21 to 22 days postbreeding and analyzed for progesterone by radioimmunoassay. Progesterone in milk greater than 11 ng/ml, 8 to 11 ng/ml, and less than 8 ng/ml was used classify animals as pregnant, questionable, and not pregnant. In this classification for 123 cows, 67 were classified pregnant, 7 questionable, and 49 not pregnant. A comparison of progesterone diagnosis with actual calving results showed agreement of 73 and 98% for the cows classified as pregnant and not pregnant. Thirteen of 19 animals, in which there were discrepancies between diagnoses by milk progesterone and calving results, were not detected in estrus within 30 days after breeding. One animal classified not pregnant by milk progesterone was pregnant.", "PMID": 956490}
{"id": "pubmed23n0032_2256", "title": "The cross-reactivity of IgE antibodies with pollen allergens. I. Analyses of various species of grass pollens.", "content": "Atopic patients with histories of grass pollen allergy often are sensitive to a variety of species of grasses. Using a serum pool from patients sensitive to June grass, we analyzed the reactivity of IgE antibodies to seven grasses by the radioallergosorbent test. Extracts were analyzed for their inhibitory activities with solid-phase allergens prepared from all of the grass pollen. Also samples of serum were exhaustively absorved with solid-phase allergens and the supernatants tested to determine the reactivity of the remaining IgE antibodies. Three patterns of reactivity were observed: (1) June, orchard, meadow fescue, and perennial rye grasses displayed similar reactivity in both inhibition and absorption studies; (2) sweet vernal and Bermuda grasses were considerably less reactive with the serum pool, indicating that they lacked antigenic determinants possessed by the other grasses; and (3) timothy grass possessed unique antigenic determinants. Knowledge of these patterns of cross-allergenicity is of importance for diagnosis and treatment of sensitive patients as well as for in vitro standardization of extracts.", "contents": "The cross-reactivity of IgE antibodies with pollen allergens. I. Analyses of various species of grass pollens. Atopic patients with histories of grass pollen allergy often are sensitive to a variety of species of grasses. Using a serum pool from patients sensitive to June grass, we analyzed the reactivity of IgE antibodies to seven grasses by the radioallergosorbent test. Extracts were analyzed for their inhibitory activities with solid-phase allergens prepared from all of the grass pollen. Also samples of serum were exhaustively absorved with solid-phase allergens and the supernatants tested to determine the reactivity of the remaining IgE antibodies. Three patterns of reactivity were observed: (1) June, orchard, meadow fescue, and perennial rye grasses displayed similar reactivity in both inhibition and absorption studies; (2) sweet vernal and Bermuda grasses were considerably less reactive with the serum pool, indicating that they lacked antigenic determinants possessed by the other grasses; and (3) timothy grass possessed unique antigenic determinants. Knowledge of these patterns of cross-allergenicity is of importance for diagnosis and treatment of sensitive patients as well as for in vitro standardization of extracts.", "PMID": 956553}
{"id": "pubmed23n0032_2257", "title": "The cross-reactivity of IgE antibodies with pollen allergens. II. Analyses of various species of ragweed and other fall weed pollens.", "content": "Although the antigenic composition of short ragweed pollen has been extensively investigated, very few studies have analyzed the allergenic cross-reactivity among various types of ragweed pollens. Using a serum pool from patients sensitive to short ragweed, we studied the cross-reactivity of IgE antibodies to six ragweeds by the radioallergosorbent test. Extracts were analyzed for their inhibitory activities with solid-phase allergens prepared from all of the ragweed pollens. Also, samples of serum were absorbed with the various solid-phase allergens and the reactivity of the remaining IgE antibodies was determined. Two patterns of reactivity were observed. Short, giant, western, and false ragweeds displayed comparable reactivity in both inhibition and absorption experiments. Slender and southern ragweed were considerably less active, indicating that they lacked allergenic groupings possessed by the other species. These same patterns of cross-reactivity were found using ragweed pollens from four commercial sources. Knowledge of these patterns of cross-allergenicity is of importance for diagnosis and treatment of sensitive patients as well as for in vitro standardization of extracts.", "contents": "The cross-reactivity of IgE antibodies with pollen allergens. II. Analyses of various species of ragweed and other fall weed pollens. Although the antigenic composition of short ragweed pollen has been extensively investigated, very few studies have analyzed the allergenic cross-reactivity among various types of ragweed pollens. Using a serum pool from patients sensitive to short ragweed, we studied the cross-reactivity of IgE antibodies to six ragweeds by the radioallergosorbent test. Extracts were analyzed for their inhibitory activities with solid-phase allergens prepared from all of the ragweed pollens. Also, samples of serum were absorbed with the various solid-phase allergens and the reactivity of the remaining IgE antibodies was determined. Two patterns of reactivity were observed. Short, giant, western, and false ragweeds displayed comparable reactivity in both inhibition and absorption experiments. Slender and southern ragweed were considerably less active, indicating that they lacked allergenic groupings possessed by the other species. These same patterns of cross-reactivity were found using ragweed pollens from four commercial sources. Knowledge of these patterns of cross-allergenicity is of importance for diagnosis and treatment of sensitive patients as well as for in vitro standardization of extracts.", "PMID": 956554}
{"id": "pubmed23n0032_2258", "title": "Transfer factor: hypoxanthine is a major component of a fraction with in vivo activity.", "content": "Transfer factor was prepared from the leukocyte lysates of four donors with known skin test reactivity. After ultrafiltration and double-gel filtration on polyacrylamide gels, fraction IV of the preparation was found to have biologic activity. This fraction contained one major and occasionally one minor ultraviolet-absorbing and zero to one ninhydrin-detectable spots on thin-layer chromatography. The major ultraviolet spot was identified as hypoxanthine. Hypoxanthine was demonstrated to be responsible for the high 260 nm/280 nm ratio of preparations with biologic activity in vivo. It was not determined if hypoxanthine is required for transfer factor activity. In addition, an orcinol-negative preparation also had biologic activity.", "contents": "Transfer factor: hypoxanthine is a major component of a fraction with in vivo activity. Transfer factor was prepared from the leukocyte lysates of four donors with known skin test reactivity. After ultrafiltration and double-gel filtration on polyacrylamide gels, fraction IV of the preparation was found to have biologic activity. This fraction contained one major and occasionally one minor ultraviolet-absorbing and zero to one ninhydrin-detectable spots on thin-layer chromatography. The major ultraviolet spot was identified as hypoxanthine. Hypoxanthine was demonstrated to be responsible for the high 260 nm/280 nm ratio of preparations with biologic activity in vivo. It was not determined if hypoxanthine is required for transfer factor activity. In addition, an orcinol-negative preparation also had biologic activity.", "PMID": 956555}
{"id": "pubmed23n0032_2259", "title": "Nonorganic adverse reactions to aeroallergen immunotherapy.", "content": "Ten patients are presented who exhibited adverse reactions to immunotherapy (IT) which mimicked immunologically mediated reactions but were thought to be nonorganic in etiology. The nonorganic nature of the reactions was considered to be established in 6 of these patients and highly likely in the remaining 4. These reactions tended to occur in young patients, particularly women, at relatively low allergen extract doses and were associated with minimal or no objective findings. There was a high incidence of coexisting or contributory psychiatric problems in these patients, particularly involving hysterical or hypochondriacal personality traits. In some patients presenting with nonorganic adverse reactions to immunotherapy, it appears that the immunotherapy may be successfully continued after the patient gains insight into the nature of the reactions. In other patients, however, these reactions may necessitate discontinuing immunotherapy, and in still other patients, these reactions may confirm the need for psychiatric consultations.", "contents": "Nonorganic adverse reactions to aeroallergen immunotherapy. Ten patients are presented who exhibited adverse reactions to immunotherapy (IT) which mimicked immunologically mediated reactions but were thought to be nonorganic in etiology. The nonorganic nature of the reactions was considered to be established in 6 of these patients and highly likely in the remaining 4. These reactions tended to occur in young patients, particularly women, at relatively low allergen extract doses and were associated with minimal or no objective findings. There was a high incidence of coexisting or contributory psychiatric problems in these patients, particularly involving hysterical or hypochondriacal personality traits. In some patients presenting with nonorganic adverse reactions to immunotherapy, it appears that the immunotherapy may be successfully continued after the patient gains insight into the nature of the reactions. In other patients, however, these reactions may necessitate discontinuing immunotherapy, and in still other patients, these reactions may confirm the need for psychiatric consultations.", "PMID": 956556}
{"id": "pubmed23n0032_2260", "title": "Cardiopulmonary effects of long-term bronchodilator administration.", "content": "Because of reports of drug tolerance occurring with chronic use of adrenergic agents in asthma, we investigated cardiopulmonary responsiveness to ephedrine and to the beta-2 agent, tebutaline, following chronic drug administration for periods up to one year. With chronic drug administration baseline (prior to daily medication) FEV increased 19% in the terbutaline group and 3.5% in the ephedrine group. An additional increase of FEV above baseline of 23% for terbutaline and 12% for ephedrine was noted after daily medication. Terbutaline also had a longer duration of effect; peak response after ephedrine occurred at 2 hr while terbutaline effect was maximal at 3 to 4 hr. There was no evidence for reduced responsiveness to the bronchodilator actions of either drug at any time. Despite prolonged use, both drugs continue to increase heart rate and pulse pressure moderately following ingestion, with a peak effect occurring at 3 hr; however, pulse pressure widened after terbutaline because of a diastolic fall, while the major effect of ephedrine was to increase systolic pressure. Baseline blood pressure, particularly diastolic, declined progressively with continued drug use. No evidence of cardiac, ehpatic, renal, or ophthalmologic toxicity or change in need for other bronchodilator agents was noted during the study with either drug.", "contents": "Cardiopulmonary effects of long-term bronchodilator administration. Because of reports of drug tolerance occurring with chronic use of adrenergic agents in asthma, we investigated cardiopulmonary responsiveness to ephedrine and to the beta-2 agent, tebutaline, following chronic drug administration for periods up to one year. With chronic drug administration baseline (prior to daily medication) FEV increased 19% in the terbutaline group and 3.5% in the ephedrine group. An additional increase of FEV above baseline of 23% for terbutaline and 12% for ephedrine was noted after daily medication. Terbutaline also had a longer duration of effect; peak response after ephedrine occurred at 2 hr while terbutaline effect was maximal at 3 to 4 hr. There was no evidence for reduced responsiveness to the bronchodilator actions of either drug at any time. Despite prolonged use, both drugs continue to increase heart rate and pulse pressure moderately following ingestion, with a peak effect occurring at 3 hr; however, pulse pressure widened after terbutaline because of a diastolic fall, while the major effect of ephedrine was to increase systolic pressure. Baseline blood pressure, particularly diastolic, declined progressively with continued drug use. No evidence of cardiac, ehpatic, renal, or ophthalmologic toxicity or change in need for other bronchodilator agents was noted during the study with either drug.", "PMID": 956557}
{"id": "pubmed23n0032_2261", "title": "Group dynamics--a point of view. People, interaction, and change.", "content": "Group dynamics is analyzed both as a science and as an art. Three major elements--people, their interaction, and the change emanating from that interaction--are discussed. People come together as a group with basic needs, such as the need to feel important. Ideally, they will feel some cooperative sense of unity; inevitably, they will interact. Passive, verbal, or restless--as the case may be--they will influence one another toward a positive or a negative goal. For a successful, total group experience, good leadership is essential, with planning to insure a climate of discovery, properly budgeted time, and realistic goals, with allowances for limitations. Group participants help each other learn, and spectators become doers who achieve goals. Guidelines, techniques, and recommended reading accompany the article.", "contents": "Group dynamics--a point of view. People, interaction, and change. Group dynamics is analyzed both as a science and as an art. Three major elements--people, their interaction, and the change emanating from that interaction--are discussed. People come together as a group with basic needs, such as the need to feel important. Ideally, they will feel some cooperative sense of unity; inevitably, they will interact. Passive, verbal, or restless--as the case may be--they will influence one another toward a positive or a negative goal. For a successful, total group experience, good leadership is essential, with planning to insure a climate of discovery, properly budgeted time, and realistic goals, with allowances for limitations. Group participants help each other learn, and spectators become doers who achieve goals. Guidelines, techniques, and recommended reading accompany the article.", "PMID": 956578}
{"id": "pubmed23n0032_2262", "title": "Sociologic study of food habits--a review. II. Differentiation, accessibility and solidarity.", "content": "The process of changing food habits is more complex than would appear to most nutritionists; it involves much more than the collection of information. Change must be studied from a multidisciplinary, chiefly a sociologic, point of view, since the food habits of families within a community are formed in a matrix of human relationships. Three major variables are presented and discussed: (a)differentiation, the capacity to process information; (b)accessibility, the measured distance between a family and an information source; and (c)solidarity, the ability of a family group to react cohesively to proposed change. To effect change in his dietary habits, an individual needs his family's cooperation. The nutritionist will do well to rely on the findings of social scientists if proposed changes in family food habits are to be implemented.", "contents": "Sociologic study of food habits--a review. II. Differentiation, accessibility and solidarity. The process of changing food habits is more complex than would appear to most nutritionists; it involves much more than the collection of information. Change must be studied from a multidisciplinary, chiefly a sociologic, point of view, since the food habits of families within a community are formed in a matrix of human relationships. Three major variables are presented and discussed: (a)differentiation, the capacity to process information; (b)accessibility, the measured distance between a family and an information source; and (c)solidarity, the ability of a family group to react cohesively to proposed change. To effect change in his dietary habits, an individual needs his family's cooperation. The nutritionist will do well to rely on the findings of social scientists if proposed changes in family food habits are to be implemented.", "PMID": 956579}
{"id": "pubmed23n0032_2263", "title": "A computer-based dietary counseling system.", "content": "A computer-based dietary counseling system is described. Designed to interact directly with patients,  the program interviews people about their dietary behavior and plans with them a weight-reducing diet of approximately 1,500 kcal. In a preliminary trial, twenty-five volunteers gave critical commentary en route through the interviews. The results provided insight into the process of dietary interviewing by computer and helped in the preparation of an experimental study (10). It is our hope that a revised and expanded version of the program will be helpful to both patients and nutritionists in the management of a variety of clinically important dietary problems.", "contents": "A computer-based dietary counseling system. A computer-based dietary counseling system is described. Designed to interact directly with patients,  the program interviews people about their dietary behavior and plans with them a weight-reducing diet of approximately 1,500 kcal. In a preliminary trial, twenty-five volunteers gave critical commentary en route through the interviews. The results provided insight into the process of dietary interviewing by computer and helped in the preparation of an experimental study (10). It is our hope that a revised and expanded version of the program will be helpful to both patients and nutritionists in the management of a variety of clinically important dietary problems.", "PMID": 956580}
{"id": "pubmed23n0032_2264", "title": "Clinical dietitians use computer-generated worksheet.", "content": "Clinical dietitians at the UMC Medical Center expressed a desire for a computer-generated listing of patients which could be used to record pertinent information obtained during patient visitation. An existing computer-stored census file was used as the data base for a computer program developed to print a dietitan's worksheet, which included the patient's name, location, and birth date. The form was printed on 8 1/2", "contents": "Clinical dietitians use computer-generated worksheet. Clinical dietitians at the UMC Medical Center expressed a desire for a computer-generated listing of patients which could be used to record pertinent information obtained during patient visitation. An existing computer-stored census file was used as the data base for a computer program developed to print a dietitan's worksheet, which included the patient's name, location, and birth date. The form was printed on 8 1/2", "PMID": 956582}
{"id": "pubmed23n0032_2265", "title": "Aging.", "content": "Aging, a wearing-out process which affects everything inanimate and animate, is accelerated by abuse of decelerated by care. The psychologic reaction of aging persons vary considerably. The concept of growing older is much more satisfactory and pleasant than the concept of being old, since the former is applicable to everyone regardless of age whereas the latter carries a finite significance as deterioration progresses. Chronologic age is much less important than biologic age. The desideratum is not longevity but rather a long, useful and meaningful life. Although heredity is of importance in longevity, it is a factor over which at present we have no control. However, we can influence the aging process according to whether we care for or abuse the body. The principal factors in accelerated aging, in order of importance, are: 1) tobacco use (particularly cigarette smoking), 2) lack of exercise, and 3) obesity. Conversely, abstinence from tobacco, exercise within tolerance, and avoidance of obesity not only decelerate aging but result in a better quality of life.", "contents": "Aging. Aging, a wearing-out process which affects everything inanimate and animate, is accelerated by abuse of decelerated by care. The psychologic reaction of aging persons vary considerably. The concept of growing older is much more satisfactory and pleasant than the concept of being old, since the former is applicable to everyone regardless of age whereas the latter carries a finite significance as deterioration progresses. Chronologic age is much less important than biologic age. The desideratum is not longevity but rather a long, useful and meaningful life. Although heredity is of importance in longevity, it is a factor over which at present we have no control. However, we can influence the aging process according to whether we care for or abuse the body. The principal factors in accelerated aging, in order of importance, are: 1) tobacco use (particularly cigarette smoking), 2) lack of exercise, and 3) obesity. Conversely, abstinence from tobacco, exercise within tolerance, and avoidance of obesity not only decelerate aging but result in a better quality of life.", "PMID": 956584}
{"id": "pubmed23n0032_2266", "title": "Postmortem study of a case of Werner's syndrome.", "content": "The postmortem study of a classic case of Werner's syndrome is presented. The subject was a Japanese man who died at the age of 39. Major findings included general microsplanchnia, extreme atrophy of the testes and skin, calcified aortic atherosclerosis, an increase of basophils in the pituitary, aspiration pneumonia, chronic pyelonephritis and a meningioma in the occipital area of the brain. Histologically, no osteoporosis was evident in the lumbar spine and iliac bone. The findings suggest that in Werner's syndrome the dominant pathologic factor may be found in connective tissue other than bone.", "contents": "Postmortem study of a case of Werner's syndrome. The postmortem study of a classic case of Werner's syndrome is presented. The subject was a Japanese man who died at the age of 39. Major findings included general microsplanchnia, extreme atrophy of the testes and skin, calcified aortic atherosclerosis, an increase of basophils in the pituitary, aspiration pneumonia, chronic pyelonephritis and a meningioma in the occipital area of the brain. Histologically, no osteoporosis was evident in the lumbar spine and iliac bone. The findings suggest that in Werner's syndrome the dominant pathologic factor may be found in connective tissue other than bone.", "PMID": 956586}
{"id": "pubmed23n0032_2267", "title": "Tachyarrhythmias in old age.", "content": "The electrocardiograms of 1,171 patients above the age of 65 in a predominantly geriatric institution were reviewed to determine the incidence of tachyarrhythmias. Data on the overall incidence and the individual types of arrhythmias are presented. Atrial fibrillation was the most common arrhythmia observed, followed by atrial flutter and supraventricular tachycardia. Atrial fibrillation often was associated with other evidence of myocardial damage. The significance of sinus bradycardia and grade I A-V block in the pathogenesis of atrial fibrillation and the significance of the tachyarrhythmias are discussed.", "contents": "Tachyarrhythmias in old age. The electrocardiograms of 1,171 patients above the age of 65 in a predominantly geriatric institution were reviewed to determine the incidence of tachyarrhythmias. Data on the overall incidence and the individual types of arrhythmias are presented. Atrial fibrillation was the most common arrhythmia observed, followed by atrial flutter and supraventricular tachycardia. Atrial fibrillation often was associated with other evidence of myocardial damage. The significance of sinus bradycardia and grade I A-V block in the pathogenesis of atrial fibrillation and the significance of the tachyarrhythmias are discussed.", "PMID": 956587}
{"id": "pubmed23n0032_2268", "title": "Hypochondriasis associated with organic brain syndrome: a new approach to therapy.", "content": "Symptoms of hypochondriasis in patients with organic brain disease usually disappear during the course of an anticoagulant-psychotherapy regimen for the treatment of dementia.", "contents": "Hypochondriasis associated with organic brain syndrome: a new approach to therapy. Symptoms of hypochondriasis in patients with organic brain disease usually disappear during the course of an anticoagulant-psychotherapy regimen for the treatment of dementia.", "PMID": 956588}
{"id": "pubmed23n0032_2269", "title": "[Acupunctural analgesia in labor. Preliminary study].", "content": "The authors report their preliminary results of the use of acupuncture analgesia in normal delivery. They obtained 22 very good results out of 31 cases of low lumbar pain, especially in posterior positions. The figures are not significant yet, but they are encouraging, as far as uterine contraction pain is concerned. The very good results obtained during caesarean section encourage us to persist with the method.", "contents": "[Acupunctural analgesia in labor. Preliminary study]. The authors report their preliminary results of the use of acupuncture analgesia in normal delivery. They obtained 22 very good results out of 31 cases of low lumbar pain, especially in posterior positions. The figures are not significant yet, but they are encouraging, as far as uterine contraction pain is concerned. The very good results obtained during caesarean section encourage us to persist with the method.", "PMID": 956607}
{"id": "pubmed23n0032_2270", "title": "[Appendicular and ureteral adenomyomas. Problem of induction of the stroma in the endometriosis foci].", "content": "The authors discuss the contents and interpretation of the theories of pathogenicity of the condition of endometriosis after having defined it in its different forms and sites. Adenomyosis (internal endometriosis) and less than adenomyomata greater than (enternal endometriosis accompanied by smooth muscle hyperplasia) give rise to special problems of histogensis. The induction of fibro-muscular stroma into the area could be brought about by two different mechanisms. There is no need for ectopic endometrial glands to induce stroma in mulleriam organs. On the other hand non-mulleriam organs under the diaphragm seem to need that the glands are present and that they induce the surrounding stroma.", "contents": "[Appendicular and ureteral adenomyomas. Problem of induction of the stroma in the endometriosis foci]. The authors discuss the contents and interpretation of the theories of pathogenicity of the condition of endometriosis after having defined it in its different forms and sites. Adenomyosis (internal endometriosis) and less than adenomyomata greater than (enternal endometriosis accompanied by smooth muscle hyperplasia) give rise to special problems of histogensis. The induction of fibro-muscular stroma into the area could be brought about by two different mechanisms. There is no need for ectopic endometrial glands to induce stroma in mulleriam organs. On the other hand non-mulleriam organs under the diaphragm seem to need that the glands are present and that they induce the surrounding stroma.", "PMID": 956609}
{"id": "pubmed23n0032_2271", "title": "[Measurement of plasma oxytocinase. Comparison with urinary excretion of estrogens].", "content": "Plasma cystinylaminopeptidase (CAP) activity and 24 h urinary excretion of oestrogens were measured simultaneously in 102 pathological pregnancies. The correlation between the two chemical assays is weak (p = 0,13, to 0,47). Plasma CAP levels were significantly (p less than 0,01) lowered in cases of fetal death; but urinary oestrogen assays were within the normal limits (p greater than 0,1) in this group. Each assay suggested an equal number of correct or incorrect prognoses. Their association resulted in 9 correct prognoses in 12 pregnancies with unfavourable outcome, but led to 12 unduly bad prognoses in a group of 40 normal births. The association of the two assays allows a more adequate diagnosis of high-risk pregnancies, but has the disadvantage of offering an unduly bad prognosis in a greater number of normal cases.", "contents": "[Measurement of plasma oxytocinase. Comparison with urinary excretion of estrogens]. Plasma cystinylaminopeptidase (CAP) activity and 24 h urinary excretion of oestrogens were measured simultaneously in 102 pathological pregnancies. The correlation between the two chemical assays is weak (p = 0,13, to 0,47). Plasma CAP levels were significantly (p less than 0,01) lowered in cases of fetal death; but urinary oestrogen assays were within the normal limits (p greater than 0,1) in this group. Each assay suggested an equal number of correct or incorrect prognoses. Their association resulted in 9 correct prognoses in 12 pregnancies with unfavourable outcome, but led to 12 unduly bad prognoses in a group of 40 normal births. The association of the two assays allows a more adequate diagnosis of high-risk pregnancies, but has the disadvantage of offering an unduly bad prognosis in a greater number of normal cases.", "PMID": 956610}
{"id": "pubmed23n0032_2272", "title": "[An original and effective technic for trachelorrhaphy. Preliminary note].", "content": "Extensive commissural tears in the region of the isthmus accompanied by incompetence of the cervix can be easily and successfully treated by an original procedure carried out through the vagina. The technique uses at the same time the general ideas and advantages of the freshening of the surfaces technique uses at the same time the general ideas and advantages of the freshening of the surfaces technique of Emmet, the repair of the isthmus technique of Lash and of Palmer and Shirodkar's cerclage technique for stitching the isthmus of the uterus.", "contents": "[An original and effective technic for trachelorrhaphy. Preliminary note]. Extensive commissural tears in the region of the isthmus accompanied by incompetence of the cervix can be easily and successfully treated by an original procedure carried out through the vagina. The technique uses at the same time the general ideas and advantages of the freshening of the surfaces technique uses at the same time the general ideas and advantages of the freshening of the surfaces technique of Emmet, the repair of the isthmus technique of Lash and of Palmer and Shirodkar's cerclage technique for stitching the isthmus of the uterus.", "PMID": 956605}
{"id": "pubmed23n0032_2273", "title": "[Activity of serum leucine aminopeptidase lamp in normal and pathologic pregnancies].", "content": "The activity of the enzyme leucine aminopeptidase (LAmP) in serum was examined in 305 pregnant women (174 women with normal pregnancy, and 131 women with disturbed pregnancy). The activity of this enzyme increases during the normal development of pregnancy. In disturbed pregnancies, the LAmP activity is lower as compared to normal pregnancies. A peculiar case: toxemia of pregnancy, where the activity of the enzyme is considerably increased. This fact may be compared with the increase of LAmP activity in cases of liver damage. The LAmP activity test in pregnancy is reliable and can be performed practically in every laboratory, thus deserving its place among the many tests meant to assess the normal function of the foeto-placental unit. This test may also help in the diagnostic of some specific pregnancies such as twin pregnancy, hydatidiform mole and extra-uterine pregnancy.", "contents": "[Activity of serum leucine aminopeptidase lamp in normal and pathologic pregnancies]. The activity of the enzyme leucine aminopeptidase (LAmP) in serum was examined in 305 pregnant women (174 women with normal pregnancy, and 131 women with disturbed pregnancy). The activity of this enzyme increases during the normal development of pregnancy. In disturbed pregnancies, the LAmP activity is lower as compared to normal pregnancies. A peculiar case: toxemia of pregnancy, where the activity of the enzyme is considerably increased. This fact may be compared with the increase of LAmP activity in cases of liver damage. The LAmP activity test in pregnancy is reliable and can be performed practically in every laboratory, thus deserving its place among the many tests meant to assess the normal function of the foeto-placental unit. This test may also help in the diagnostic of some specific pregnancies such as twin pregnancy, hydatidiform mole and extra-uterine pregnancy.", "PMID": 956611}
{"id": "pubmed23n0032_2274", "title": "[Surgical treatment of cancer of the vulva. Our experience apropos of 21 cases].", "content": "The authors report the anatomoclinical aspects and the treatment of 21 cases of cancer of vulva at the Danang General Hosptial from 1961 to 1974. The treatment is only surgical : total vulvectomy and bilateral femoral, inguinal and pelvic lymphadenectomies (radical vulvectomy). The 5 year survival is 63,3 p. 100. Stages I and II have a better survival rate. Metastasis in nodes determine a poor prognosis.", "contents": "[Surgical treatment of cancer of the vulva. Our experience apropos of 21 cases]. The authors report the anatomoclinical aspects and the treatment of 21 cases of cancer of vulva at the Danang General Hosptial from 1961 to 1974. The treatment is only surgical : total vulvectomy and bilateral femoral, inguinal and pelvic lymphadenectomies (radical vulvectomy). The 5 year survival is 63,3 p. 100. Stages I and II have a better survival rate. Metastasis in nodes determine a poor prognosis.", "PMID": 956606}
{"id": "pubmed23n0032_2275", "title": "[Obstetrical tocography. I. Didactic summary].", "content": "The importance and value of manometry are presented with examples of patients in labour. It is important to distinguish between the pressures and the forces working on the fetus, especially as far as the causes of fetal bradycardia are concerned. Graphs of magnetic lag are shown in order to demonstrate that the tension on the fetus and sometimes on the placenta and the cord precedes and lasts longer than the increase in hydrostatic pressure which accompanies a contraction. The paper includes the following subjects: Introduction and general observations. The patient and the monitoring device and the tolerance to external and internal manometry by the patient. The concepts of pressure and force. The distrubution of pressures and forces in obstetrics. The measure of forces by their conversion into pressure in a manometric system. The interpretation of the results of the measure of pressure and force in obstetrics.", "contents": "[Obstetrical tocography. I. Didactic summary]. The importance and value of manometry are presented with examples of patients in labour. It is important to distinguish between the pressures and the forces working on the fetus, especially as far as the causes of fetal bradycardia are concerned. Graphs of magnetic lag are shown in order to demonstrate that the tension on the fetus and sometimes on the placenta and the cord precedes and lasts longer than the increase in hydrostatic pressure which accompanies a contraction. The paper includes the following subjects: Introduction and general observations. The patient and the monitoring device and the tolerance to external and internal manometry by the patient. The concepts of pressure and force. The distrubution of pressures and forces in obstetrics. The measure of forces by their conversion into pressure in a manometric system. The interpretation of the results of the measure of pressure and force in obstetrics.", "PMID": 956612}
{"id": "pubmed23n0032_2276", "title": "[Histologic and immunologic study of the testis after ligation of the vas deferens in dogs].", "content": "We wanted to study the effect of bilateral ligature and section of the deferentia of 12 dogs from the histological and immunological aspects. We looked for circulating anti-sperm antibodies and anti-basal membrane of the testes antibodies at the same time as histological study to find out what happened to these lesions after epididymo-deferential anastomosis had been carried out 16 weeks later. The histological lesions (disappearance of exfoliation of the germ cells and of necrobiosis of these cells) disapperared after anastomosis. Anti-basal membrane antibodies were never found. Anti-testicular circulating auto-antibodies were never found. Anti-sperm antibodies appeared between 1 and 5 months after ligature and then disappeared a few months later. Failures to fertilise after epiddidymo-deferential anastomoses therefore do not seem to be due to these two factors.", "contents": "[Histologic and immunologic study of the testis after ligation of the vas deferens in dogs]. We wanted to study the effect of bilateral ligature and section of the deferentia of 12 dogs from the histological and immunological aspects. We looked for circulating anti-sperm antibodies and anti-basal membrane of the testes antibodies at the same time as histological study to find out what happened to these lesions after epididymo-deferential anastomosis had been carried out 16 weeks later. The histological lesions (disappearance of exfoliation of the germ cells and of necrobiosis of these cells) disapperared after anastomosis. Anti-basal membrane antibodies were never found. Anti-testicular circulating auto-antibodies were never found. Anti-sperm antibodies appeared between 1 and 5 months after ligature and then disappeared a few months later. Failures to fertilise after epiddidymo-deferential anastomoses therefore do not seem to be due to these two factors.", "PMID": 956613}
{"id": "pubmed23n0032_2277", "title": "[Automatic analyses of rapid fluctuations of basal fetal heart rate during delivery. Verification of parameters].", "content": "The authors using an electronic calculator have measured 3 parameters, which allowed them to carry out quantitative evaluations of rapid variations in basal fetal cardiac rhythms (fluctuation) of 29 samples of fetal heart rate obtained during labour. The three parameters are: 1. Mean variation by intervals. 2. Values for differences in intervals approaching zero. 3. Zero changes in the derived curve. Each of these parameters was compared with the values of fluctuations which were obtained by visual analysis quantified on the same samples of tracings. The results of these coefficients of correlation show that the chosen parameters enable quantitative values for these fluctuations to be obtained.", "contents": "[Automatic analyses of rapid fluctuations of basal fetal heart rate during delivery. Verification of parameters]. The authors using an electronic calculator have measured 3 parameters, which allowed them to carry out quantitative evaluations of rapid variations in basal fetal cardiac rhythms (fluctuation) of 29 samples of fetal heart rate obtained during labour. The three parameters are: 1. Mean variation by intervals. 2. Values for differences in intervals approaching zero. 3. Zero changes in the derived curve. Each of these parameters was compared with the values of fluctuations which were obtained by visual analysis quantified on the same samples of tracings. The results of these coefficients of correlation show that the chosen parameters enable quantitative values for these fluctuations to be obtained.", "PMID": 956614}
{"id": "pubmed23n0032_2278", "title": "[Laparotomies during pregnancy. Apropos of 32 cases].", "content": "Among the 5612 pregnancies followed at the 2nd Obstetrical and Gynaecological Clinic of the University Hospital of Athens, from July 1969 to December 1974, laparotomy was undertaken 32 times for conditions not associated with pregnancy, an incidence of 0,58%. The laparotomy was performed because of ovarian cysts, fibroids, acute appendicitis and bowel obstruction. The accurate diagnosis, the fetal loss rate, maternal morbidity and mortality were discussed.", "contents": "[Laparotomies during pregnancy. Apropos of 32 cases]. Among the 5612 pregnancies followed at the 2nd Obstetrical and Gynaecological Clinic of the University Hospital of Athens, from July 1969 to December 1974, laparotomy was undertaken 32 times for conditions not associated with pregnancy, an incidence of 0,58%. The laparotomy was performed because of ovarian cysts, fibroids, acute appendicitis and bowel obstruction. The accurate diagnosis, the fetal loss rate, maternal morbidity and mortality were discussed.", "PMID": 956616}
{"id": "pubmed23n0032_2279", "title": "[Intraepithelial carcinoma of the cervix uteri. 1) Cytologic detection in gynecologic practice. Our exerience with 15,000 smears].", "content": "The best results in screening for subclinical cancer of the cervix are given by cytological studies. The authors in reviewing their experience in this method of screening studied the result of 15,000 smears which gave a diagnosis of 37 cases of intra-epithelial carcinoma of the cervix, which means 1 case of carcinoma in situ diagnosed in every 400 smears. Although the technique is very simple it has to be carried out according to strict criteria at the time of taking the smear, of fixing it immediately and of staining it. There is a group of patients who are at high risk about the age of forty, when there is a marked influence due to parity. But only a systematic policy will bring about diagnosis of pre-invasive carcinoma in cases where the cervix is clinically healthy. The cytology is usually characteristic, though the diagnosis may be difficult during pregnancy, or when hormone contraception is being used or when there is a trichomonas infection present. This explains why we find false positives in 0.03 per cent of cases in this study and false negatives in 0.03 per cent of cases. Their rarity means that the method is 99.94 per cent reliable. Finally, the cervical smear gives an opportunity for studying the vaginal microbial flora as well as the cyto-hormonal state.", "contents": "[Intraepithelial carcinoma of the cervix uteri. 1) Cytologic detection in gynecologic practice. Our exerience with 15,000 smears]. The best results in screening for subclinical cancer of the cervix are given by cytological studies. The authors in reviewing their experience in this method of screening studied the result of 15,000 smears which gave a diagnosis of 37 cases of intra-epithelial carcinoma of the cervix, which means 1 case of carcinoma in situ diagnosed in every 400 smears. Although the technique is very simple it has to be carried out according to strict criteria at the time of taking the smear, of fixing it immediately and of staining it. There is a group of patients who are at high risk about the age of forty, when there is a marked influence due to parity. But only a systematic policy will bring about diagnosis of pre-invasive carcinoma in cases where the cervix is clinically healthy. The cytology is usually characteristic, though the diagnosis may be difficult during pregnancy, or when hormone contraception is being used or when there is a trichomonas infection present. This explains why we find false positives in 0.03 per cent of cases in this study and false negatives in 0.03 per cent of cases. Their rarity means that the method is 99.94 per cent reliable. Finally, the cervical smear gives an opportunity for studying the vaginal microbial flora as well as the cyto-hormonal state.", "PMID": 956615}
{"id": "pubmed23n0032_2280", "title": "[Development of ultrastructural changes in human cervix mucus during the ovarian cycle. Scanning electron microscope study].", "content": "The use of the Scanning Electron Microscope has made it possible through observation to study the human cervical mucus through the various stages of the ovarian cycle, as well as to describe the significant variations of the meshed woof making up the ultrastructure during the ovarian cycle. While the slackening of the woof and the dimension of the meshes are minimal at both the beginning and end of the cycle, they reach a maximum on forteenth day. In the ovulatory period, lateral expansions from the filaments are numerous. On the other hand, median and terminal thicknesses are almost inexistant during the same period : their frequency decreases during the first part of the cycle, then increases during the luteal phase. During both the preceding and following days of ovulation, one can observe numerous twistings at the level of the filaments which probably express the relaxation and then contraction of the latter. The preparatory technique as well as the method of observation used appear reliable enough to allow a comparison of the cervical mucus ultrastructure in varied physiological, pathological and experimental situations.", "contents": "[Development of ultrastructural changes in human cervix mucus during the ovarian cycle. Scanning electron microscope study]. The use of the Scanning Electron Microscope has made it possible through observation to study the human cervical mucus through the various stages of the ovarian cycle, as well as to describe the significant variations of the meshed woof making up the ultrastructure during the ovarian cycle. While the slackening of the woof and the dimension of the meshes are minimal at both the beginning and end of the cycle, they reach a maximum on forteenth day. In the ovulatory period, lateral expansions from the filaments are numerous. On the other hand, median and terminal thicknesses are almost inexistant during the same period : their frequency decreases during the first part of the cycle, then increases during the luteal phase. During both the preceding and following days of ovulation, one can observe numerous twistings at the level of the filaments which probably express the relaxation and then contraction of the latter. The preparatory technique as well as the method of observation used appear reliable enough to allow a comparison of the cervical mucus ultrastructure in varied physiological, pathological and experimental situations.", "PMID": 956619}
{"id": "pubmed23n0032_2281", "title": "[Acute pancreatitis and pregnancy].", "content": "When a pregnant woman presented with a syndrome suggesting obstruction and fever together with jaundice and oliguria the authors remembered the difficulty of clinical diagnosis and the biology of pancreatitis. They studied the connection of this condition with pregnancy, its pathogenesis, its prognosis and its treatment, as well as the various factors that were favourable (treatment with diuretics, the right ovarian vein syndrome).", "contents": "[Acute pancreatitis and pregnancy]. When a pregnant woman presented with a syndrome suggesting obstruction and fever together with jaundice and oliguria the authors remembered the difficulty of clinical diagnosis and the biology of pancreatitis. They studied the connection of this condition with pregnancy, its pathogenesis, its prognosis and its treatment, as well as the various factors that were favourable (treatment with diuretics, the right ovarian vein syndrome).", "PMID": 956617}
{"id": "pubmed23n0032_2282", "title": "[Listerosis and pregnancy without fetal disease].", "content": "A case of vaginal listeriosis occurring in the sixth month of pregnancy is reported. It was discovered when the patient suffered from symptoms of appendicitis complicated by jaundice and an acute nephritis. It was investigated by vaginal smears and swabs, and by amniocentesis. Treatment was general and local. The fetus was not attacked, the pregnancy continued, resulting in the delivery and birth of an undamaged infant.", "contents": "[Listerosis and pregnancy without fetal disease]. A case of vaginal listeriosis occurring in the sixth month of pregnancy is reported. It was discovered when the patient suffered from symptoms of appendicitis complicated by jaundice and an acute nephritis. It was investigated by vaginal smears and swabs, and by amniocentesis. Treatment was general and local. The fetus was not attacked, the pregnancy continued, resulting in the delivery and birth of an undamaged infant.", "PMID": 956623}
{"id": "pubmed23n0032_2283", "title": "[Attempt at a standardized interpretation of testicular histopathology in male sterility].", "content": "The authors present a standard for interpreting the histopathology of testicular biopsies carried out during the framework of investigation of male sterility. The standard is based on a study of the alterations in the seminiferous tubes (T), the peritubular membranes (M) and the interstitial tissue (I). This method of histological assessment has been used to study 35 subjects on whom bilateral bipolar biopsies were carried out (130 specimens). The statistical study showed: a) the absence of any difference between the pieces taken from the two poles in each testis; b) the level of concentration of spermatozoa could not be tied up with any of the three histological parameters. This applied also for hormone levels with the exception of GUT which seem to correlate directly with M; c) the presence of a varicocoele does not reflect in any way the membrane or the interstitial tissue; d) spermograms carried out after the biopsies showed that testicular biopsy does not cause permanent trauma to the testis. The advantages and disadvantages of this quantitative method are discussed.", "contents": "[Attempt at a standardized interpretation of testicular histopathology in male sterility]. The authors present a standard for interpreting the histopathology of testicular biopsies carried out during the framework of investigation of male sterility. The standard is based on a study of the alterations in the seminiferous tubes (T), the peritubular membranes (M) and the interstitial tissue (I). This method of histological assessment has been used to study 35 subjects on whom bilateral bipolar biopsies were carried out (130 specimens). The statistical study showed: a) the absence of any difference between the pieces taken from the two poles in each testis; b) the level of concentration of spermatozoa could not be tied up with any of the three histological parameters. This applied also for hormone levels with the exception of GUT which seem to correlate directly with M; c) the presence of a varicocoele does not reflect in any way the membrane or the interstitial tissue; d) spermograms carried out after the biopsies showed that testicular biopsy does not cause permanent trauma to the testis. The advantages and disadvantages of this quantitative method are discussed.", "PMID": 956629}
{"id": "pubmed23n0032_2284", "title": "[Bismuth encephalopathy in pregnant women. Apropos of a case].", "content": "A case of bismuth encephalopathy in a pregnant woman is reported. The neurological picture, characterized especially by myoclonic attacks, is no different from that found in the non-pregnant state. The principal observations are on three points: - the need to think of a toxic origin of convulsions in pregnancy, - the onset of threatened premature labour during repeated convulsive crises, - the passage of bismuth across the placenta : this has been proved by the levels found in the amniotic fluid (55 mcg per 1,000) and in the cord blood (320 mcg per 1,000). The child, who was normal at birth, developed a transient hypotonus which seemed attributable more to the treatment of the mother with Diazepam and pheno-barbitone than to her having taken bismuth during the pregnancy.", "contents": "[Bismuth encephalopathy in pregnant women. Apropos of a case]. A case of bismuth encephalopathy in a pregnant woman is reported. The neurological picture, characterized especially by myoclonic attacks, is no different from that found in the non-pregnant state. The principal observations are on three points: - the need to think of a toxic origin of convulsions in pregnancy, - the onset of threatened premature labour during repeated convulsive crises, - the passage of bismuth across the placenta : this has been proved by the levels found in the amniotic fluid (55 mcg per 1,000) and in the cord blood (320 mcg per 1,000). The child, who was normal at birth, developed a transient hypotonus which seemed attributable more to the treatment of the mother with Diazepam and pheno-barbitone than to her having taken bismuth during the pregnancy.", "PMID": 956618}
{"id": "pubmed23n0032_2285", "title": "[Intra-epithelial carcinoma of the cervix uteri. Our experience with treatment, the role of conization].", "content": "The authors re-emphasize that the sure diagnosis of carcinoma of the cervix depends on histology; having considered a series of 15,000 smears in an article on cytological screening for carcinoma of the cervix. They show that the main prop of this histological examination should definitely be conisation. They furthermore prepare an inventory of the different possible methods of treatment of intra-epithelial carcinoma, stating for each method the advantages and disadvantages of the method. Their attitude to treatment is conservative, and they believe that conisation in every case is sufficient when complete removal of the in situ lesion is carried out whatever the age of the patient is. They state the limits of this method and define clearly the contra-indications for such a scheme of treatment, which can only be carried out routinely with the absolutely necessary cooperation of a cytology laboratory and faultless histology.", "contents": "[Intra-epithelial carcinoma of the cervix uteri. Our experience with treatment, the role of conization]. The authors re-emphasize that the sure diagnosis of carcinoma of the cervix depends on histology; having considered a series of 15,000 smears in an article on cytological screening for carcinoma of the cervix. They show that the main prop of this histological examination should definitely be conisation. They furthermore prepare an inventory of the different possible methods of treatment of intra-epithelial carcinoma, stating for each method the advantages and disadvantages of the method. Their attitude to treatment is conservative, and they believe that conisation in every case is sufficient when complete removal of the in situ lesion is carried out whatever the age of the patient is. They state the limits of this method and define clearly the contra-indications for such a scheme of treatment, which can only be carried out routinely with the absolutely necessary cooperation of a cytology laboratory and faultless histology.", "PMID": 956625}
{"id": "pubmed23n0032_2286", "title": "[Simultaneous study of the placental, myometrial and cervical circulations (indium 113 and anemometric thermometry). 2. Effect of xanthinol nicotinate].", "content": "The authors have studied the action of nicotinate of xantinol on the placental, myometrial and cervical circulations using an isotope technique (Indium 113) and anemometric thermometry. They show that this product in every case increases the flow through the cervix and has little action on the flow through the placenta, except that in rare cases it lessens it. The action on the myometrial circulation varies from woman to woman. There is a significant negative statistical correlation between variation in the circulation through the cervix and the placenta.", "contents": "[Simultaneous study of the placental, myometrial and cervical circulations (indium 113 and anemometric thermometry). 2. Effect of xanthinol nicotinate]. The authors have studied the action of nicotinate of xantinol on the placental, myometrial and cervical circulations using an isotope technique (Indium 113) and anemometric thermometry. They show that this product in every case increases the flow through the cervix and has little action on the flow through the placenta, except that in rare cases it lessens it. The action on the myometrial circulation varies from woman to woman. There is a significant negative statistical correlation between variation in the circulation through the cervix and the placenta.", "PMID": 956630}
{"id": "pubmed23n0032_2287", "title": "[Contractile force, work of the uterus, power developed and resistance of the cervix uteri during labor. Measurements in Poissy units].", "content": "Apart from records made of uterine contractions in labour and the progress in dilatation of the cervix, the energy exerted by the uterus to open the cervix can be calculated. The power that is developed and the resistance of the factors that combine to inhibit dilatation of the cervix can also be calculated. Examples are given of promiparous women with a dilatation of 5 cm.", "contents": "[Contractile force, work of the uterus, power developed and resistance of the cervix uteri during labor. Measurements in Poissy units]. Apart from records made of uterine contractions in labour and the progress in dilatation of the cervix, the energy exerted by the uterus to open the cervix can be calculated. The power that is developed and the resistance of the factors that combine to inhibit dilatation of the cervix can also be calculated. Examples are given of promiparous women with a dilatation of 5 cm.", "PMID": 956620}
{"id": "pubmed23n0032_2288", "title": "[Electronic surveillance during delivery. A method of reading the tracings].", "content": "A method for reading the foetal heart rate tracings and a guide of management advise in each case have been established from a review of the literature and the study of 500 records. The tracings are schematically divided into three categories according to the presumed risk of foetal distress: normal or \"tolerable\" tracings, alarm symptoms and danger symptoms. For each categorie, and according to the duration of the variations, a type of management is proposed. Other diagnostic means, such as capillary blood pH measurement are desirable to improve the foetal state diagnostic and make the treatment more precise.", "contents": "[Electronic surveillance during delivery. A method of reading the tracings]. A method for reading the foetal heart rate tracings and a guide of management advise in each case have been established from a review of the literature and the study of 500 records. The tracings are schematically divided into three categories according to the presumed risk of foetal distress: normal or \"tolerable\" tracings, alarm symptoms and danger symptoms. For each categorie, and according to the duration of the variations, a type of management is proposed. Other diagnostic means, such as capillary blood pH measurement are desirable to improve the foetal state diagnostic and make the treatment more precise.", "PMID": 956631}
{"id": "pubmed23n0032_2289", "title": "[Obstetrical tocography. II. Review of its methodology and a new concept].", "content": "The methods employed for measuring pressure have been analysed from the historical and practical points of view. The systems at present in use are criticised and a modification based on catheters with inflatable balloons is described. By this method precise manometry is obtained using a strong material. The patient can unplug the monitoring system without it having to be recalibrated later. Furthermore this system avoids errors which come from movements of the catheter, changes of temperature and accidental increases in pressures. Equally it will stand humidity and sterilisation by heat.", "contents": "[Obstetrical tocography. II. Review of its methodology and a new concept]. The methods employed for measuring pressure have been analysed from the historical and practical points of view. The systems at present in use are criticised and a modification based on catheters with inflatable balloons is described. By this method precise manometry is obtained using a strong material. The patient can unplug the monitoring system without it having to be recalibrated later. Furthermore this system avoids errors which come from movements of the catheter, changes of temperature and accidental increases in pressures. Equally it will stand humidity and sterilisation by heat.", "PMID": 956621}
{"id": "pubmed23n0032_2290", "title": "[Uncommon form of vesico-uterine fistula].", "content": "A vesico-uterine fistula has been seen after a caesarean section. Unusually, the discharge ran from the bladder to the uterus. The problems of the aetiology, the diagnosis and the treatment are discussed.", "contents": "[Uncommon form of vesico-uterine fistula]. A vesico-uterine fistula has been seen after a caesarean section. Unusually, the discharge ran from the bladder to the uterus. The problems of the aetiology, the diagnosis and the treatment are discussed.", "PMID": 956632}
{"id": "pubmed23n0032_2291", "title": "[Loco-regional analgesia by electronic stimulation during labor].", "content": "This is a preliminary report on an original method of local regional electronic analgesia. From it the first results can be deduced. The threshold for pain which was appreciated at 25 mmHg without electronic stimulation was raised to 48 mm Hg under electronic stimulation. Fetal ill-effects are nil.", "contents": "[Loco-regional analgesia by electronic stimulation during labor]. This is a preliminary report on an original method of local regional electronic analgesia. From it the first results can be deduced. The threshold for pain which was appreciated at 25 mmHg without electronic stimulation was raised to 48 mm Hg under electronic stimulation. Fetal ill-effects are nil.", "PMID": 956626}
{"id": "pubmed23n0032_2292", "title": "[Measurements by ultrasonics of the gestation sac in threatened abortion].", "content": "The embryonic sac has been measured and its dimensions compared with normal measurements in cases where symptoms suggest threatened abortion. This has been done with the object of trying to complete visual exploration of the uterine contents in such cases. The distribution of the diameters that have been measured in 148 pathological cases show that the number of those below the lower limit of normal is significantly higher when pregnancy will result in abortion or in premature labour.", "contents": "[Measurements by ultrasonics of the gestation sac in threatened abortion]. The embryonic sac has been measured and its dimensions compared with normal measurements in cases where symptoms suggest threatened abortion. This has been done with the object of trying to complete visual exploration of the uterine contents in such cases. The distribution of the diameters that have been measured in 148 pathological cases show that the number of those below the lower limit of normal is significantly higher when pregnancy will result in abortion or in premature labour.", "PMID": 956622}
{"id": "pubmed23n0032_2293", "title": "[Benign mesothelioma of the genital area].", "content": "A case of benign mesothelioma of the genital tract which required two operations to cure is reported. The patient is in a satisfactory condition ten years after the second operation. A bibliography is given.", "contents": "[Benign mesothelioma of the genital area]. A case of benign mesothelioma of the genital tract which required two operations to cure is reported. The patient is in a satisfactory condition ten years after the second operation. A bibliography is given.", "PMID": 956628}
{"id": "pubmed23n0032_2294", "title": "[Detection of phthiriasis by colposcopy].", "content": "The colposcope need not be used solely to examine the mucosa of the cervix and vagina. It can help in the diagnosis of the etiology of genito-pubic phthiriasis by the finding of louse eggs firmly fixed to the hairs, whereas usually only the low magnification microscope shows them up.", "contents": "[Detection of phthiriasis by colposcopy]. The colposcope need not be used solely to examine the mucosa of the cervix and vagina. It can help in the diagnosis of the etiology of genito-pubic phthiriasis by the finding of louse eggs firmly fixed to the hairs, whereas usually only the low magnification microscope shows them up.", "PMID": 956633}
{"id": "pubmed23n0032_2295", "title": "[Tetanus and pregnancy. Labor with assisted respiration].", "content": "The authors report a rare case in which tetanus occurred with a pregnancy near term. In this particular case of pregnancy with risk for the fetus and mother associated with one another, curative treatment with monitoring of the state of the fetus and assisted respiration for the mother resulted in the birth of a live child who could be brought up normally and a cure for the mother without having to resort to caesarean section.", "contents": "[Tetanus and pregnancy. Labor with assisted respiration]. The authors report a rare case in which tetanus occurred with a pregnancy near term. In this particular case of pregnancy with risk for the fetus and mother associated with one another, curative treatment with monitoring of the state of the fetus and assisted respiration for the mother resulted in the birth of a live child who could be brought up normally and a cure for the mother without having to resort to caesarean section.", "PMID": 956634}
{"id": "pubmed23n0032_2296", "title": "[Macroscopic examination of the fresh placenta. Statistical analysis of 1,200 cases. Comparison with maternal and neonatal pathology].", "content": "We have examined 1,200 placentas in a fresh state and have compared the results of these examinations with maternal pathology on the one hand and the neo-natal pathology on the other hand. It turns out that most abnormalities of pregnancy are associated in a significant way with the pathological findings in the placenta, and that the good or pathological health of the newborn is significantly associated with the normal of pathological character of the placenta. The principal weakness in this method of examination is the absence of information about infection. This is why it seems important to us to carry out smears from the placenta before examining it macroscopically.", "contents": "[Macroscopic examination of the fresh placenta. Statistical analysis of 1,200 cases. Comparison with maternal and neonatal pathology]. We have examined 1,200 placentas in a fresh state and have compared the results of these examinations with maternal pathology on the one hand and the neo-natal pathology on the other hand. It turns out that most abnormalities of pregnancy are associated in a significant way with the pathological findings in the placenta, and that the good or pathological health of the newborn is significantly associated with the normal of pathological character of the placenta. The principal weakness in this method of examination is the absence of information about infection. This is why it seems important to us to carry out smears from the placenta before examining it macroscopically.", "PMID": 956624}
{"id": "pubmed23n0032_2297", "title": "[Development of the breasts of the fetus. Effects of the administration of hormonal preparations during pregnancy].", "content": "The mammary glands of ten still-birth premature fetuses were studied histologically. In seven cases a combination of oestrogen-progesterone preparations were administered to the mother in early pregnancy for a duration of at least 3 months. In three cases no hormones were administered. According to our observations and the experimental work of other investigators, we may conclude that oestrogenic hormones inhibit the development of the mammary gland.", "contents": "[Development of the breasts of the fetus. Effects of the administration of hormonal preparations during pregnancy]. The mammary glands of ten still-birth premature fetuses were studied histologically. In seven cases a combination of oestrogen-progesterone preparations were administered to the mother in early pregnancy for a duration of at least 3 months. In three cases no hormones were administered. According to our observations and the experimental work of other investigators, we may conclude that oestrogenic hormones inhibit the development of the mammary gland.", "PMID": 956635}
{"id": "pubmed23n0032_2298", "title": "[Indications of choice for extra-amniotic perfusion of physiologic serum].", "content": "After a preliminary report on 20 cases, we have used extra-amniotic perfusion of isotonic saline, in association or not with syntocinon, for performing:--56 2nd trimester therapeutic abortions,--16 labor inductions in interrupted 2nd and 3rd trimester pregnancies, with macerated fetus (missed abortion and missed labor). This method, relatively simple, permits the discharge of the conceptus within a reasonable time. Our experience allowed us to draw the indications of choice in the use of this method, which are the following: 1. When the amniotic puncture may be difficult (at the beginning of the 4th month or if the age of pregnancy is uncertain). 2. When there is an increased risk of hemorrhage with coagulation disorder macerated fetus or some systemic diseases). 3. When there are counter-indications for the use of hypertonic saline solution or prostaglandins (some renal, cardiac or respiratory diseases). 4. When one needs a fetus in a relatively good state for embryologic, histologic or genetic studies, which are important in case of abortion for rubella or chromosomic anomaly. In view of the innocuousness of this method, its use for labor induction in evolutive pregnancies is suggested.", "contents": "[Indications of choice for extra-amniotic perfusion of physiologic serum]. After a preliminary report on 20 cases, we have used extra-amniotic perfusion of isotonic saline, in association or not with syntocinon, for performing:--56 2nd trimester therapeutic abortions,--16 labor inductions in interrupted 2nd and 3rd trimester pregnancies, with macerated fetus (missed abortion and missed labor). This method, relatively simple, permits the discharge of the conceptus within a reasonable time. Our experience allowed us to draw the indications of choice in the use of this method, which are the following: 1. When the amniotic puncture may be difficult (at the beginning of the 4th month or if the age of pregnancy is uncertain). 2. When there is an increased risk of hemorrhage with coagulation disorder macerated fetus or some systemic diseases). 3. When there are counter-indications for the use of hypertonic saline solution or prostaglandins (some renal, cardiac or respiratory diseases). 4. When one needs a fetus in a relatively good state for embryologic, histologic or genetic studies, which are important in case of abortion for rubella or chromosomic anomaly. In view of the innocuousness of this method, its use for labor induction in evolutive pregnancies is suggested.", "PMID": 956636}
{"id": "pubmed23n0032_2299", "title": "[Transverse laparotomy by supre-pubic detachment of the recti muscles in gynecologic surgery. Apropos of 266 cases].", "content": "The authors have studied 266 case histories where supra-pubic detachment of rectus muscles was used according to Cherny's method. This allowed aseptic gynaecological surgery to be carried out in all its forms by a true transverse incision. The advantages are great : it is aesthetic and the incision can be enlarged if necessary indefinitely. This means that surgery can be carried out easily and explains the great rarity of intra-abdominal complications. On the other hand two relatively rare complications do occur with this incision : peritoneal haematomata and lateral herniations. Details have been given of the methods necessary to avoid these complications.", "contents": "[Transverse laparotomy by supre-pubic detachment of the recti muscles in gynecologic surgery. Apropos of 266 cases]. The authors have studied 266 case histories where supra-pubic detachment of rectus muscles was used according to Cherny's method. This allowed aseptic gynaecological surgery to be carried out in all its forms by a true transverse incision. The advantages are great : it is aesthetic and the incision can be enlarged if necessary indefinitely. This means that surgery can be carried out easily and explains the great rarity of intra-abdominal complications. On the other hand two relatively rare complications do occur with this incision : peritoneal haematomata and lateral herniations. Details have been given of the methods necessary to avoid these complications.", "PMID": 956627}
{"id": "pubmed23n0032_2300", "title": "Sequential protein analysis from single identified neurons of Aplysia californica. A microelectrophoretic technique involving polyacrylamide gradient gels and isoelectric focusing.", "content": "Electrophoresis in polyacrylamide gradient gels and isoelectric focusing in polyacrylamide gels of capillary size are powerful tools for the analysis of molecular weight and charge properties of small protein samples. This is demonstrated using identified neurons from the abdominal ganglion of the sea hare Aplysia californica. Certain cell-specific peptides, which are considered to be neurosecretory, have been shown to be water soluble when ethylene glycol was employed as a mobilizing agent. Although the mode of action of ethylene glycol is not yet understood, this method may be of value for various extraction procedures. The application of a new staining method that is preferential for separations of sodium dodecyl sulfate-proteins yields information about the charge of water-insoluble proteins which has so far been inaccessible. Preliminary results gained by a small, two-dimensional mapping procedure as well as optical density separation patterns of two different nuclear protein fractionation from a single isolated nucleus outline further possibilties of the microgel techniques.", "contents": "Sequential protein analysis from single identified neurons of Aplysia californica. A microelectrophoretic technique involving polyacrylamide gradient gels and isoelectric focusing. Electrophoresis in polyacrylamide gradient gels and isoelectric focusing in polyacrylamide gels of capillary size are powerful tools for the analysis of molecular weight and charge properties of small protein samples. This is demonstrated using identified neurons from the abdominal ganglion of the sea hare Aplysia californica. Certain cell-specific peptides, which are considered to be neurosecretory, have been shown to be water soluble when ethylene glycol was employed as a mobilizing agent. Although the mode of action of ethylene glycol is not yet understood, this method may be of value for various extraction procedures. The application of a new staining method that is preferential for separations of sodium dodecyl sulfate-proteins yields information about the charge of water-insoluble proteins which has so far been inaccessible. Preliminary results gained by a small, two-dimensional mapping procedure as well as optical density separation patterns of two different nuclear protein fractionation from a single isolated nucleus outline further possibilties of the microgel techniques.", "PMID": 956643}
{"id": "pubmed23n0032_2301", "title": "Ultrastructural localization of catalase and L-alpha-hydroxy acid oxidase in microperoxisomes of Hydra.", "content": "The ultrastructural localization of catalase and L-alpha-hydroxy acid oxidase (LalphaHAO) was studied in two species of Hydra. Diaminobenzidine reaction product of catalase activity was present in small round or elongated bodies resembling microperoxisomes in the epitheliomuscular, digestive and gland cells. They were closely related to the endoplasmic reticulum, and were often found in proximity to deposits of lipid and glycogen. Reaction product of LalphaHAO activity was also associated with the microperoxisomes. With rapidly oxidized substrates, such as L-lactic acid, reaction product diffused into the cytoplasm around the microperoxisomes. With slowly oxidized substrates, such as DL-alpha-hydroxyisovaleric acid, reaction product was restricted to the matrix of the microperoxisomes. No reaction product was present in the microperoxisomes in the absence of substrate or with D-lactic acid. The rate of substrate oxidation measured biochemically roughly paralleled the amount of cytochemical reaction product deposited with different substrates. Microperoxisome-like bodies reactive for LalphaHAO were also found in the epidermal cnidoblasts; however, catalase could not be demonstrated in them. This study provides the first cytochemical evidence for the presence of an H2O2-producing oxidase in microperoxisomes.", "contents": "Ultrastructural localization of catalase and L-alpha-hydroxy acid oxidase in microperoxisomes of Hydra. The ultrastructural localization of catalase and L-alpha-hydroxy acid oxidase (LalphaHAO) was studied in two species of Hydra. Diaminobenzidine reaction product of catalase activity was present in small round or elongated bodies resembling microperoxisomes in the epitheliomuscular, digestive and gland cells. They were closely related to the endoplasmic reticulum, and were often found in proximity to deposits of lipid and glycogen. Reaction product of LalphaHAO activity was also associated with the microperoxisomes. With rapidly oxidized substrates, such as L-lactic acid, reaction product diffused into the cytoplasm around the microperoxisomes. With slowly oxidized substrates, such as DL-alpha-hydroxyisovaleric acid, reaction product was restricted to the matrix of the microperoxisomes. No reaction product was present in the microperoxisomes in the absence of substrate or with D-lactic acid. The rate of substrate oxidation measured biochemically roughly paralleled the amount of cytochemical reaction product deposited with different substrates. Microperoxisome-like bodies reactive for LalphaHAO were also found in the epidermal cnidoblasts; however, catalase could not be demonstrated in them. This study provides the first cytochemical evidence for the presence of an H2O2-producing oxidase in microperoxisomes.", "PMID": 956644}
{"id": "pubmed23n0032_2302", "title": "Lymphocytic choriomeningitis virus-induced immunosuppression: a virus-induced macrophage defect.", "content": "Primary immunizing infections with LCM virus result in a transient depression of the in vitro proliferative responses of splenic lymphocytes to mitogens specifically reactive with T cells or B cells. This depression of lymphocyte function is the result of a virus-induced defect in an adherent, phagocytic cell population required for in vitro lymphocyte activation. Depressed responses persist for about 1 week after virus clearance and can be corrected by the addition of normal PEM or 2-ME to infected spleen cell cultures. Although the precise nature of this defect remains unclear, it is postulated that it is due to a productive infection of macrophages and their precursors that renders them dysfunctional. Secondary LCM virus infections do not result in depressed in vitro responses to mitogens, presumably because of rapid virus clearance and limited numbers of infected cells. Primary infections of immunologically immature mice, mice rendered functionally athymic, or mice treated with nonspecific immunosuppressive agents result in LCM virus persistence. Shortly after infection, these animals show a similar depression of immunologic reactivity that returns to normal as the virus carrier state becomes established. Despite virus persistence, few PEM from established LCM virus carrier mice contain viral antigens and these cells function normally. Thus, LCM virus-induced immunosuppression appears to reflect a subtle cytopathic effect of LCM virus replication that is not mediated by the virus-specific cell-mediated immune mechanisms responsible for acute LCM virus disease.", "contents": "Lymphocytic choriomeningitis virus-induced immunosuppression: a virus-induced macrophage defect. Primary immunizing infections with LCM virus result in a transient depression of the in vitro proliferative responses of splenic lymphocytes to mitogens specifically reactive with T cells or B cells. This depression of lymphocyte function is the result of a virus-induced defect in an adherent, phagocytic cell population required for in vitro lymphocyte activation. Depressed responses persist for about 1 week after virus clearance and can be corrected by the addition of normal PEM or 2-ME to infected spleen cell cultures. Although the precise nature of this defect remains unclear, it is postulated that it is due to a productive infection of macrophages and their precursors that renders them dysfunctional. Secondary LCM virus infections do not result in depressed in vitro responses to mitogens, presumably because of rapid virus clearance and limited numbers of infected cells. Primary infections of immunologically immature mice, mice rendered functionally athymic, or mice treated with nonspecific immunosuppressive agents result in LCM virus persistence. Shortly after infection, these animals show a similar depression of immunologic reactivity that returns to normal as the virus carrier state becomes established. Despite virus persistence, few PEM from established LCM virus carrier mice contain viral antigens and these cells function normally. Thus, LCM virus-induced immunosuppression appears to reflect a subtle cytopathic effect of LCM virus replication that is not mediated by the virus-specific cell-mediated immune mechanisms responsible for acute LCM virus disease.", "PMID": 956645}
{"id": "pubmed23n0032_2303", "title": "Colchicine-sensitive structures and lymphocyte activation.", "content": "The possible modulatory role of microtubules or closely related colchicine-sensitive structures in the response of human lymphocytes to mitogenic lectins was investigated. Colchicine (0.1 to 10 muM) AND VINBLASTINE (0.1 TO 10 MUM) inhibited early [14C]-aminoisobutyric acid and late [3H]-thymidine uptake in phytohemagglutinin-and concanavalin A-stimulated human lymphocytes but failed to alter 45Ca uptake. Lumicolchicine, an inactive congener of colchicine, was ineffective in all three systems. Both microtubular agents accentuated and prolonged the early cyclic AMP response to lectin. Little or no alteration in cyclic AMP levels was seen with colchicine or vinblastine alone or in combination with PGE (10MUM) or epinephrine (1muM) suggesting that the effect on cyclic AMP metabolism is largely selective for lectin stimulation. Neither microtunular agent altered 125I-concanacalin A binding. Since the inhibition of DNA synthesis was throughout the culture period and early aminoisobutyric acid uptake is affected, it appears that these agents are acting on an early event, or events, in the activation sequence. Although the mechanism of the inhibition is not known, the effect of colchicine and vinblastine in prolonging the cyclic AMP response to lectin may be involved. Alternatively, alterations in microtubules assembly may exert effects on membrane architecture interfering with propagation of the stimulus from the membrane to the cell interior.", "contents": "Colchicine-sensitive structures and lymphocyte activation. The possible modulatory role of microtubules or closely related colchicine-sensitive structures in the response of human lymphocytes to mitogenic lectins was investigated. Colchicine (0.1 to 10 muM) AND VINBLASTINE (0.1 TO 10 MUM) inhibited early [14C]-aminoisobutyric acid and late [3H]-thymidine uptake in phytohemagglutinin-and concanavalin A-stimulated human lymphocytes but failed to alter 45Ca uptake. Lumicolchicine, an inactive congener of colchicine, was ineffective in all three systems. Both microtubular agents accentuated and prolonged the early cyclic AMP response to lectin. Little or no alteration in cyclic AMP levels was seen with colchicine or vinblastine alone or in combination with PGE (10MUM) or epinephrine (1muM) suggesting that the effect on cyclic AMP metabolism is largely selective for lectin stimulation. Neither microtunular agent altered 125I-concanacalin A binding. Since the inhibition of DNA synthesis was throughout the culture period and early aminoisobutyric acid uptake is affected, it appears that these agents are acting on an early event, or events, in the activation sequence. Although the mechanism of the inhibition is not known, the effect of colchicine and vinblastine in prolonging the cyclic AMP response to lectin may be involved. Alternatively, alterations in microtubules assembly may exert effects on membrane architecture interfering with propagation of the stimulus from the membrane to the cell interior.", "PMID": 956646}
{"id": "pubmed23n0032_2304", "title": "Intestinal uptake of macromolecules. V. Comparison of the in vitro uptake by rat small intestine of antigen-antibody complexes prepared in antibody or antigen excess.", "content": "The in vitro absorption by rat jejunal and ileal gut sacs of soluble antigen-antibody complexes and of antigen alone was compared. Complexes prepared in 2-fold antibody excess were absorbed in significantly smaller quantities than was antigen alone. Complexes prepared in 50-fold antigen excess were absorbed by jejunal gut sacs in quantities equivalent to that antigen alone, whereas the absorption of such complexes by ileal sacs was somewhat decreased compared to that of antigen. There was less radiolabeled antigen tightly bound to the intestinal mucosa of gut sacs exposed to complexes prepared in antigen or antibody excess compared to antigen alone. Complexes prepared in antibody excess appeared to stimulate secretion of mucus and complexes were associated with the mucus fraction. It was suggested that the large size of complexes prepared in antibody excess may result in their trapping in the mucus coat of the gut, thereby preventing contact with the surface of the enterocyte from whence uptake by pinocytosis is initiated. In addition, complexes appear to be shed from the surface with mucus by a mechanism still to be elucidated.", "contents": "Intestinal uptake of macromolecules. V. Comparison of the in vitro uptake by rat small intestine of antigen-antibody complexes prepared in antibody or antigen excess. The in vitro absorption by rat jejunal and ileal gut sacs of soluble antigen-antibody complexes and of antigen alone was compared. Complexes prepared in 2-fold antibody excess were absorbed in significantly smaller quantities than was antigen alone. Complexes prepared in 50-fold antigen excess were absorbed by jejunal gut sacs in quantities equivalent to that antigen alone, whereas the absorption of such complexes by ileal sacs was somewhat decreased compared to that of antigen. There was less radiolabeled antigen tightly bound to the intestinal mucosa of gut sacs exposed to complexes prepared in antigen or antibody excess compared to antigen alone. Complexes prepared in antibody excess appeared to stimulate secretion of mucus and complexes were associated with the mucus fraction. It was suggested that the large size of complexes prepared in antibody excess may result in their trapping in the mucus coat of the gut, thereby preventing contact with the surface of the enterocyte from whence uptake by pinocytosis is initiated. In addition, complexes appear to be shed from the surface with mucus by a mechanism still to be elucidated.", "PMID": 956647}
{"id": "pubmed23n0032_2305", "title": "Immunochemical studies of infectious mononucleosis. V. Isolation and characterization of a glycoprotein from goat erythrocyte membranes.", "content": "A glycoprotein was isolated from goat erythrocyte membranes by extraction with hot 75% ethanol. The glycoprotein was purified by ethanol precipitation, phosphocellulose chromatography gel filtration, ethyl:ether and chloroform:methanol extraction. In aqueous phosphate-buffered saline, pH 7, the glycoprotein was in an aggregated state with a sedimentation coefficient (S(obs)) to 1.5. Electrophoresis of the glycoprotein on polyacrylamide gels containing phosphate-buffered 0.1% SDS gave a single band, staining with both periodic acid Schiff (PAS) and Coomassie Blue (CB). The apparent m.w., calculated from retardation coefficient, was 25,000. Electrophoresis of the glycoprotein on 1% SDS gels buffered with Tris-acetate (pH 7.4) showed a major band of similar (23,500) apparent m.w. plus four other PAS- and CB-staining bands of lower mobility. With 131I-labeled glycoprotein, recovery of bands from gels, sialic acid analysis, heterophile antigen activity, and re-electrophoresis, it was shown that these additional bands were aggregated forms of a single or closely related glycoprotein species. The purified glycoprotein contained 50% carbohydrate with molar ratios of sialic acid:galactose:mannose:galactosamine:glucosamine of 3.1:2.1:0.1:1.6:1. The glycoprotein was highly reactive with the Paul-Bunnell heterophile antibody in the sera of patients with infectious mononucleosis, with Limulus polyphemus lectin and weakly ractive with wheat germ agglutinin. These reactivities were destroyed by neuraminidase treatment or by alkaline sodium borohydride. The native glycoprotein did not react with lectins from Canavalia enisformis, Phaseolus vulgaris, Ricinus communis, or Vicia graminea although it was reactive with the latter two after neuraminidase treatment.", "contents": "Immunochemical studies of infectious mononucleosis. V. Isolation and characterization of a glycoprotein from goat erythrocyte membranes. A glycoprotein was isolated from goat erythrocyte membranes by extraction with hot 75% ethanol. The glycoprotein was purified by ethanol precipitation, phosphocellulose chromatography gel filtration, ethyl:ether and chloroform:methanol extraction. In aqueous phosphate-buffered saline, pH 7, the glycoprotein was in an aggregated state with a sedimentation coefficient (S(obs)) to 1.5. Electrophoresis of the glycoprotein on polyacrylamide gels containing phosphate-buffered 0.1% SDS gave a single band, staining with both periodic acid Schiff (PAS) and Coomassie Blue (CB). The apparent m.w., calculated from retardation coefficient, was 25,000. Electrophoresis of the glycoprotein on 1% SDS gels buffered with Tris-acetate (pH 7.4) showed a major band of similar (23,500) apparent m.w. plus four other PAS- and CB-staining bands of lower mobility. With 131I-labeled glycoprotein, recovery of bands from gels, sialic acid analysis, heterophile antigen activity, and re-electrophoresis, it was shown that these additional bands were aggregated forms of a single or closely related glycoprotein species. The purified glycoprotein contained 50% carbohydrate with molar ratios of sialic acid:galactose:mannose:galactosamine:glucosamine of 3.1:2.1:0.1:1.6:1. The glycoprotein was highly reactive with the Paul-Bunnell heterophile antibody in the sera of patients with infectious mononucleosis, with Limulus polyphemus lectin and weakly ractive with wheat germ agglutinin. These reactivities were destroyed by neuraminidase treatment or by alkaline sodium borohydride. The native glycoprotein did not react with lectins from Canavalia enisformis, Phaseolus vulgaris, Ricinus communis, or Vicia graminea although it was reactive with the latter two after neuraminidase treatment.", "PMID": 956650}
{"id": "pubmed23n0032_2306", "title": "In vitro induction of F1 hybrid anti-parent cell-mediated cytotoxicity.", "content": "Spleen cells from normal (C57BL/6 X DBA/2)F1 mice were sensitized in vitro for 5 days with irradiated C57BL/6 or DBA/2 parental stimulating cells. Effector cells were generated which specifically lysed 51Cr-labeled targets (leukemia or mitogen-stimulated lymphoid cells) H-2-matched with the parental genotype used for sensitization. The response of F1 spleen cells to the C57BL/6 parent was stronger and more reproducible than that to the DBA/2 parent. The kinetics of generation of effector cells were similar for the F1 anti-parent and an F1 anti-allogeneic response. However, the magnitude of the F1 anti-C57BL/6 cytotoxic response was considerably lower than the F1 response to allogeneic cells. The ratio of responder to stimulator cells in the cultures was more critical for the former than for the latter response. Several lots of fetal bovine serum were found to be adequate for supplementing the medium in the induction of J1 hybrid anti-parent and anti-allogeneic cytotoxic effector cells. Based on these and other studies, it would appear that the F1 hybrid anti-parent cytotoxic response provides an in vitro model of murine hemopoietic graft rejection in vivo. This response may be elicited by a mechanism distinct from T cell-mediated cytotoxicity and involve different subpopulations of spleen cells.", "contents": "In vitro induction of F1 hybrid anti-parent cell-mediated cytotoxicity. Spleen cells from normal (C57BL/6 X DBA/2)F1 mice were sensitized in vitro for 5 days with irradiated C57BL/6 or DBA/2 parental stimulating cells. Effector cells were generated which specifically lysed 51Cr-labeled targets (leukemia or mitogen-stimulated lymphoid cells) H-2-matched with the parental genotype used for sensitization. The response of F1 spleen cells to the C57BL/6 parent was stronger and more reproducible than that to the DBA/2 parent. The kinetics of generation of effector cells were similar for the F1 anti-parent and an F1 anti-allogeneic response. However, the magnitude of the F1 anti-C57BL/6 cytotoxic response was considerably lower than the F1 response to allogeneic cells. The ratio of responder to stimulator cells in the cultures was more critical for the former than for the latter response. Several lots of fetal bovine serum were found to be adequate for supplementing the medium in the induction of J1 hybrid anti-parent and anti-allogeneic cytotoxic effector cells. Based on these and other studies, it would appear that the F1 hybrid anti-parent cytotoxic response provides an in vitro model of murine hemopoietic graft rejection in vivo. This response may be elicited by a mechanism distinct from T cell-mediated cytotoxicity and involve different subpopulations of spleen cells.", "PMID": 956651}
{"id": "pubmed23n0032_2307", "title": "HLA-related control of spontaneous and antibody-dependent cell-mediated cytotoxic activity in humans.", "content": "Normal human lymphocyte preparations were tested for their ability to lyse both antibody-coated and unsensitized human target cell lines. The capacity to induce these two types of cell-mediated cytotoxicity, antibody-dependent (Ab-CMC) and spontaneous (Sp-CMC), was detected by a 51Cr release assay. The reactivity of lymphocytes from 1. individual donors showed a positive and highly significanlty correlation between Ab-CMC and Sp-CMC, leading to the hypothesis that the same type of effector cell is involved in the two cytotoxic mechanisms. Lymphocytes from male donors were about twice as effective as those from female donors in both systems. Moreover, the effector cells from male donors carrying HLA antigens A3 and B7 displayed a significantly lower reactivity in both Sp-CMC and Ab-CMC when compared with lymphocytes from male donors bearing any other HLA haplotype. The possible significance of the hyporeactivity of lymphocytes from normal subjects with HLA-A3,B7 haplotype in relation to an increased susceptibility to multiple sclerosis (MS) is discussed.", "contents": "HLA-related control of spontaneous and antibody-dependent cell-mediated cytotoxic activity in humans. Normal human lymphocyte preparations were tested for their ability to lyse both antibody-coated and unsensitized human target cell lines. The capacity to induce these two types of cell-mediated cytotoxicity, antibody-dependent (Ab-CMC) and spontaneous (Sp-CMC), was detected by a 51Cr release assay. The reactivity of lymphocytes from 1. individual donors showed a positive and highly significanlty correlation between Ab-CMC and Sp-CMC, leading to the hypothesis that the same type of effector cell is involved in the two cytotoxic mechanisms. Lymphocytes from male donors were about twice as effective as those from female donors in both systems. Moreover, the effector cells from male donors carrying HLA antigens A3 and B7 displayed a significantly lower reactivity in both Sp-CMC and Ab-CMC when compared with lymphocytes from male donors bearing any other HLA haplotype. The possible significance of the hyporeactivity of lymphocytes from normal subjects with HLA-A3,B7 haplotype in relation to an increased susceptibility to multiple sclerosis (MS) is discussed.", "PMID": 956652}
{"id": "pubmed23n0032_2308", "title": "Human transfer factor: effects on lymphocyte transformation.", "content": "Transfer factor preparations from 57 different donors have been compared for effects on mitogen- and antigen-induced lymphocyte transformation. Nine of the preparations were mitogenic when added to cultured lymphocytes although the magnitude of this activity was relatively low. The majority of the preparations (48/57) did not affect PHA-induced lymphocyte transformation although augmentation (6 of 57) and suppression (3 of 57) was observed with some. In addition we observed that most of the preparations tested suppressed ConA stimulation and augmented the PWM response. When selected preparations were evaluated on antigen-responsive cells, there was a correlation between the magnitude of antigen responsiveness and the magnitude of TF augmentation of antigen-induced lymphocyte transformation (p less than 0.005). Cultures that were not responsive to antigen (KLH-negative or BUdR-treated) could not be stimulated by TF from immune donors and antigen. These data suggest that TF preparations contain either stimulatory or inhibitory components and that TF is not capable of activating naive lymphocytes to undergo transformation in response to antigen.", "contents": "Human transfer factor: effects on lymphocyte transformation. Transfer factor preparations from 57 different donors have been compared for effects on mitogen- and antigen-induced lymphocyte transformation. Nine of the preparations were mitogenic when added to cultured lymphocytes although the magnitude of this activity was relatively low. The majority of the preparations (48/57) did not affect PHA-induced lymphocyte transformation although augmentation (6 of 57) and suppression (3 of 57) was observed with some. In addition we observed that most of the preparations tested suppressed ConA stimulation and augmented the PWM response. When selected preparations were evaluated on antigen-responsive cells, there was a correlation between the magnitude of antigen responsiveness and the magnitude of TF augmentation of antigen-induced lymphocyte transformation (p less than 0.005). Cultures that were not responsive to antigen (KLH-negative or BUdR-treated) could not be stimulated by TF from immune donors and antigen. These data suggest that TF preparations contain either stimulatory or inhibitory components and that TF is not capable of activating naive lymphocytes to undergo transformation in response to antigen.", "PMID": 956653}
{"id": "pubmed23n0032_2309", "title": "Antibody-induced killing in vivo of L1210/MTX-R cells quantitated in passively immunized mice with 131I-iododeoxyuridine-labeled cells and whole-body measurement of retained radioactivity.", "content": "The killing of the LR subline of the DBA/2J leukemia L1210/MTX by passive antibody was followed in vivo with 131I-iododeoxyuridine-labeled cells and whole-body measurement of retained radioactivity. The in vivo killing of LR cells was proportional to the in vitro 2-mercaptoethanol resistant titer, independent of the complement system, and radioresistant. Although a large percentage of the leukemic cells was killed in passively immunized mice, the protective effect of the passive antiserum was dependent on the active immune response of the host.", "contents": "Antibody-induced killing in vivo of L1210/MTX-R cells quantitated in passively immunized mice with 131I-iododeoxyuridine-labeled cells and whole-body measurement of retained radioactivity. The killing of the LR subline of the DBA/2J leukemia L1210/MTX by passive antibody was followed in vivo with 131I-iododeoxyuridine-labeled cells and whole-body measurement of retained radioactivity. The in vivo killing of LR cells was proportional to the in vitro 2-mercaptoethanol resistant titer, independent of the complement system, and radioresistant. Although a large percentage of the leukemic cells was killed in passively immunized mice, the protective effect of the passive antiserum was dependent on the active immune response of the host.", "PMID": 956654}
{"id": "pubmed23n0032_2310", "title": "A new activity of complement component C3: cell-bound C3b potentiates lysis of erythrocytes by C5b,6 and terminal components.", "content": "EAC4b,3b (sheep erythrocytes carrying rabbit antibody and guinea pig complement component fragments C4b and C3) adsorb human C5b,6 reversibly; the avidity of binding varies inversely with ionic strength. We believe that the receptor of C5b,6 is contributed by the cell-bound C3b because the binding capacity of EAC4b,3b varies with C3b multiplicity and can be blocked with rabbit antibody to guinea pig C3. The fixation of C5b,6 to the erythrocyte-bound C3b serves to concentrate C5b,6 on the cell surface; as a consequence, the hemolytic efficiency of C5b,6 is almost 100 times greater when assayed with EAC4b,3b than with plain erythrocytes. This potentiation represents a hitherto unrecognized function of cell-bound C3b.", "contents": "A new activity of complement component C3: cell-bound C3b potentiates lysis of erythrocytes by C5b,6 and terminal components. EAC4b,3b (sheep erythrocytes carrying rabbit antibody and guinea pig complement component fragments C4b and C3) adsorb human C5b,6 reversibly; the avidity of binding varies inversely with ionic strength. We believe that the receptor of C5b,6 is contributed by the cell-bound C3b because the binding capacity of EAC4b,3b varies with C3b multiplicity and can be blocked with rabbit antibody to guinea pig C3. The fixation of C5b,6 to the erythrocyte-bound C3b serves to concentrate C5b,6 on the cell surface; as a consequence, the hemolytic efficiency of C5b,6 is almost 100 times greater when assayed with EAC4b,3b than with plain erythrocytes. This potentiation represents a hitherto unrecognized function of cell-bound C3b.", "PMID": 956655}
{"id": "pubmed23n0032_2311", "title": "Immune response in the hamster. VIII. Ig class differences in susceptibility to tolerance induction.", "content": "A hemolytic plaque assay was used to quantitate the antibody response of the Syrian hamster after immunization with hen egg albumin (HEA). Whereas HEA in complete Freund's adjuvant (HEA-CF) induced a prolonged heterogeneous (IgM, IgG1 and IgG2) antibody response, the response to soluble HEA in saline (HEA-S) differed in that: 1)both the primary and secondary responses were restricted to the IgG1 class; 2)the IgG1 primary response was cyclical with PFC peaks on days 9 and 16; 3)although an anamnestic secondary response was demonstrated, no further augmentation was noted after tertiary and quaternary boosters; 4)the booster response was transient reaching a peak after 48 hr and declining to low levels within 7 days. Adoptive transfer of lymph node cells to irradiated recipients followed by challenge with HEA-CF revealed: 1)that HEA-S-treated donor cells were primed for an IgG1 response because anamnesis was seen 7 days after challenge, yet on day 21, IgG1 PFC were 20-fold less than that of controls; 2)recipients of HEA-S treated cells showed profound suppression of both IgM and IgG2 PFC on days 14 and 21. These studies indicate that soluble antigen induced in hamsters a state of complete tolerance of IgM and IgG2 classes whereas the anamnestic response of the IgG1 class remained intact.", "contents": "Immune response in the hamster. VIII. Ig class differences in susceptibility to tolerance induction. A hemolytic plaque assay was used to quantitate the antibody response of the Syrian hamster after immunization with hen egg albumin (HEA). Whereas HEA in complete Freund's adjuvant (HEA-CF) induced a prolonged heterogeneous (IgM, IgG1 and IgG2) antibody response, the response to soluble HEA in saline (HEA-S) differed in that: 1)both the primary and secondary responses were restricted to the IgG1 class; 2)the IgG1 primary response was cyclical with PFC peaks on days 9 and 16; 3)although an anamnestic secondary response was demonstrated, no further augmentation was noted after tertiary and quaternary boosters; 4)the booster response was transient reaching a peak after 48 hr and declining to low levels within 7 days. Adoptive transfer of lymph node cells to irradiated recipients followed by challenge with HEA-CF revealed: 1)that HEA-S-treated donor cells were primed for an IgG1 response because anamnesis was seen 7 days after challenge, yet on day 21, IgG1 PFC were 20-fold less than that of controls; 2)recipients of HEA-S treated cells showed profound suppression of both IgM and IgG2 PFC on days 14 and 21. These studies indicate that soluble antigen induced in hamsters a state of complete tolerance of IgM and IgG2 classes whereas the anamnestic response of the IgG1 class remained intact.", "PMID": 956656}
{"id": "pubmed23n0032_2312", "title": "Influenza A virus interaction with murine lymphocytes. II. Changes in lymphocyte surface properties induced by influenza virus A/Japan 305 (H2N2).", "content": "This report describes changes in surface properties of rat thoracic duct lymphocytes (TDL) induced by influenza virus A/Japan 305 (H2N2). After incubation with virus at 37 degrees C, sialic acids were released from the membranes of TDL WITH CONCOMItant reduction in their mean electrophoretic mobility. Autoradiographs of TDL which had been incubated with 125I virus at 4 degrees C showed that the particles attached to nearly all the lymphocytes although there was variation in the amount of virus bound per cell. Attachment occurred rapidly with maximum labeling evident after incubation at 4 degrees C for 5 min. Incubation at 37 degrees C for 1 hr resulted in a 10 to 18% reduction in the incidence of labeled TDL suggesting that some virus eluted spontaneously. When 125I-virus-treated TDL were incubated in syngeneic serum, substantial amounts of virus eluted reducing the incidence of labeled cells by 50%. In addition, evidence was obtained by indirect immunofluorescence that syngeneic serum contains antibodies which react with sites on the lymphocyte surface exposed by the viral neuraminidase.", "contents": "Influenza A virus interaction with murine lymphocytes. II. Changes in lymphocyte surface properties induced by influenza virus A/Japan 305 (H2N2). This report describes changes in surface properties of rat thoracic duct lymphocytes (TDL) induced by influenza virus A/Japan 305 (H2N2). After incubation with virus at 37 degrees C, sialic acids were released from the membranes of TDL WITH CONCOMItant reduction in their mean electrophoretic mobility. Autoradiographs of TDL which had been incubated with 125I virus at 4 degrees C showed that the particles attached to nearly all the lymphocytes although there was variation in the amount of virus bound per cell. Attachment occurred rapidly with maximum labeling evident after incubation at 4 degrees C for 5 min. Incubation at 37 degrees C for 1 hr resulted in a 10 to 18% reduction in the incidence of labeled TDL suggesting that some virus eluted spontaneously. When 125I-virus-treated TDL were incubated in syngeneic serum, substantial amounts of virus eluted reducing the incidence of labeled cells by 50%. In addition, evidence was obtained by indirect immunofluorescence that syngeneic serum contains antibodies which react with sites on the lymphocyte surface exposed by the viral neuraminidase.", "PMID": 956657}
{"id": "pubmed23n0032_2313", "title": "Structure and function of immunoglobulin domains. V. Binding, University of immunoglobulin G and fragments to placental membrane preparations.", "content": "Fc receptors have been shown to be present in human placental tissue with properties distinct from those on macrophages and lymphocytes. A single class of receptor was observed with an intrinsic affinity 4X 10(6) M-(1) for human IgG1. The order of affinity for IgG subclasses was IgG1 = IGG1 greater than IgG3 greater than IgG4. IgA and IgM were not bound. Fc from IgG1 bound with the same affinity as the whole molecule and to the same number of receptor sites, 2 X 10 (12)/mg placental protein. IgG1 was no longer boung after mild reduction and alkylation whereas the binding of Fc was unaffected by this treatment. Neithe C3 nor C3, the two domains which comprise the Fc region of IgG1, bound to the placental receptor. This implies that this Fc receptor is unlike those found on most cell surfaces and that plasental binding is an exception to the theory that each domain has evoked to perform independent functions.", "contents": "Structure and function of immunoglobulin domains. V. Binding, University of immunoglobulin G and fragments to placental membrane preparations. Fc receptors have been shown to be present in human placental tissue with properties distinct from those on macrophages and lymphocytes. A single class of receptor was observed with an intrinsic affinity 4X 10(6) M-(1) for human IgG1. The order of affinity for IgG subclasses was IgG1 = IGG1 greater than IgG3 greater than IgG4. IgA and IgM were not bound. Fc from IgG1 bound with the same affinity as the whole molecule and to the same number of receptor sites, 2 X 10 (12)/mg placental protein. IgG1 was no longer boung after mild reduction and alkylation whereas the binding of Fc was unaffected by this treatment. Neithe C3 nor C3, the two domains which comprise the Fc region of IgG1, bound to the placental receptor. This implies that this Fc receptor is unlike those found on most cell surfaces and that plasental binding is an exception to the theory that each domain has evoked to perform independent functions.", "PMID": 956658}
{"id": "pubmed23n0032_2314", "title": "Evidence for the presence of components of the alternative (properdin) pathway of complement activation in respiratory secretions.", "content": "Activation of the alternative complement pathway by respiratory secretory IgA was demonstrated by incubating purified, aggregated preparations of serum and secretory IgA with neat human serum. No depletion of the early components (C1-4) was observed, but 63 and 70% of C3-9, respectively, were consumed. The C3-9-consuming capacity of heat-aggregated nasal secretions from an IgA-deficient volunteer was compared with heat-aggregated nasal secretions from a normal volunteer known to have secretory IgA. The deficient secretions consumed C3-9, whereas the IgA deficient secretions did not. Reconstitution of the nasal secretions from the IgA-deficient volunteer with purified secretory IgA produced alternative pathway activation. Factor B of the alternative complement pathway was found to be present in 16 of 18 bronchoalveolar lavage samples (BALF) from normal volunteers. Simultaneous measurement of lavage and serum albumin and Factor B concentrations rendered it unlikely that Factor B was merely a transudative product from serum in half the samples but rather suggested that it may be a component of lower respiratory tract secretions. The presence of an intact alternative complement pathway in BALF was indicated by showing that cobra venom factor and endotoxin cleaved functionally pure human C3 when mixed with BALF, but had no effect on C3 in the absence of BALF.", "contents": "Evidence for the presence of components of the alternative (properdin) pathway of complement activation in respiratory secretions. Activation of the alternative complement pathway by respiratory secretory IgA was demonstrated by incubating purified, aggregated preparations of serum and secretory IgA with neat human serum. No depletion of the early components (C1-4) was observed, but 63 and 70% of C3-9, respectively, were consumed. The C3-9-consuming capacity of heat-aggregated nasal secretions from an IgA-deficient volunteer was compared with heat-aggregated nasal secretions from a normal volunteer known to have secretory IgA. The deficient secretions consumed C3-9, whereas the IgA deficient secretions did not. Reconstitution of the nasal secretions from the IgA-deficient volunteer with purified secretory IgA produced alternative pathway activation. Factor B of the alternative complement pathway was found to be present in 16 of 18 bronchoalveolar lavage samples (BALF) from normal volunteers. Simultaneous measurement of lavage and serum albumin and Factor B concentrations rendered it unlikely that Factor B was merely a transudative product from serum in half the samples but rather suggested that it may be a component of lower respiratory tract secretions. The presence of an intact alternative complement pathway in BALF was indicated by showing that cobra venom factor and endotoxin cleaved functionally pure human C3 when mixed with BALF, but had no effect on C3 in the absence of BALF.", "PMID": 956659}
{"id": "pubmed23n0032_2315", "title": "An indirect radioimmunoassay for thymus leukemia (TL) antigens.", "content": "An indirect radioimmunassay for thymus leukemia (TL)2 antigens has been developed and its specificity documented. The assay makes use of anti-TL antibodies produced in congenic mice (A-Tla(b)) and radioiodinated purified rabbit anti-mouse IgG. Using this assay, differences can be detected in the amounts of antigen expressed on thymocytes of the three known phenotypes (TL.1,2,3;TL.2;TL-) of inbred mouse strains. Significant differences are also detected in comparison of the thymocytes from homozygous TL.1,2,3 mice (A-Tla(a)) and heterozyhotes from Tla(a) and Tla(b) parents. Optimum conditions for the assay have been established. Its ability to detect antigens on glutaraldehyde-fixed cells and the binding of noncytolytic antibodies on both viable and fixed cells are documented. The assay has also been used to quantitate the changes in TL antigen expression on cells incubated in anti-TL antisera under conditions of antigenic modulation.", "contents": "An indirect radioimmunoassay for thymus leukemia (TL) antigens. An indirect radioimmunassay for thymus leukemia (TL)2 antigens has been developed and its specificity documented. The assay makes use of anti-TL antibodies produced in congenic mice (A-Tla(b)) and radioiodinated purified rabbit anti-mouse IgG. Using this assay, differences can be detected in the amounts of antigen expressed on thymocytes of the three known phenotypes (TL.1,2,3;TL.2;TL-) of inbred mouse strains. Significant differences are also detected in comparison of the thymocytes from homozygous TL.1,2,3 mice (A-Tla(a)) and heterozyhotes from Tla(a) and Tla(b) parents. Optimum conditions for the assay have been established. Its ability to detect antigens on glutaraldehyde-fixed cells and the binding of noncytolytic antibodies on both viable and fixed cells are documented. The assay has also been used to quantitate the changes in TL antigen expression on cells incubated in anti-TL antisera under conditions of antigenic modulation.", "PMID": 956660}
{"id": "pubmed23n0032_2316", "title": "The complement profile in babesiosis.", "content": "Rats have been infected with Babesia rodhaini, a protozoan agent that induces immune complex disease. Assays for complement have revealed depletion of C2, C3, C4, C5, and whole complement in the course of infection. No evidence of depletion in the alternative (properdin) pathway was found. These findings are consistent with the conclusion that the classical complement pathway is activated during the course of the protozoan infection.", "contents": "The complement profile in babesiosis. Rats have been infected with Babesia rodhaini, a protozoan agent that induces immune complex disease. Assays for complement have revealed depletion of C2, C3, C4, C5, and whole complement in the course of infection. No evidence of depletion in the alternative (properdin) pathway was found. These findings are consistent with the conclusion that the classical complement pathway is activated during the course of the protozoan infection.", "PMID": 956661}
{"id": "pubmed23n0032_2317", "title": "Humoral immunostimulation. VI. Increased calcium uptake by cells treated with antibody and complement.", "content": "When L cells were treated with anti-L cell antibody in medium depleted of complement, rapid increases in calcium uptake were obtained over a wide range of antiserum concentrations. Concomitant cell growth and viability studies demonstrated that stimulation of cell growth occurred at higher dilutions of antiserum whereas cytotoxicity occurred at lower dilutions. The stimulatory and toxic effects of antibody on cell growth were potentiated by complement as was the enhancement in calcium uptake. Sera deficient in C1R, C2,4D, C4, C3-C9 did not increase the calcium uptake response to antibody whereas augmentation did occur with C6-deficient serum. A specific role for complement was further indicated by the ability of purified complement components to restore the response to complement in complement-deficient sera. C3 with C3-C9 deficient serum, but not C2, C5, and C6 with C3-C9 deficient serum restored augmentation effects. Taken together with the results of previous studies it is apparent that complement augments both calcium and nucleoside uptake and that the effect is primarily via the classical complement pathway through C3. Substrate saturation studies demonstrated that antibody activated the facilitated diffusion of calcium altering the Vmax but not the Km of transport whereas addition of complement altered both the Vmax and Km. These findings suggest that one of the early effects of enhancing antibody upon tumor cell metabolism in vitro is to stimulate uptake of calcium. In view of the suspected role of Ca++ in cell proliferation the increase in cell-associated calcium may be important in the subsequent proliferative response.", "contents": "Humoral immunostimulation. VI. Increased calcium uptake by cells treated with antibody and complement. When L cells were treated with anti-L cell antibody in medium depleted of complement, rapid increases in calcium uptake were obtained over a wide range of antiserum concentrations. Concomitant cell growth and viability studies demonstrated that stimulation of cell growth occurred at higher dilutions of antiserum whereas cytotoxicity occurred at lower dilutions. The stimulatory and toxic effects of antibody on cell growth were potentiated by complement as was the enhancement in calcium uptake. Sera deficient in C1R, C2,4D, C4, C3-C9 did not increase the calcium uptake response to antibody whereas augmentation did occur with C6-deficient serum. A specific role for complement was further indicated by the ability of purified complement components to restore the response to complement in complement-deficient sera. C3 with C3-C9 deficient serum, but not C2, C5, and C6 with C3-C9 deficient serum restored augmentation effects. Taken together with the results of previous studies it is apparent that complement augments both calcium and nucleoside uptake and that the effect is primarily via the classical complement pathway through C3. Substrate saturation studies demonstrated that antibody activated the facilitated diffusion of calcium altering the Vmax but not the Km of transport whereas addition of complement altered both the Vmax and Km. These findings suggest that one of the early effects of enhancing antibody upon tumor cell metabolism in vitro is to stimulate uptake of calcium. In view of the suspected role of Ca++ in cell proliferation the increase in cell-associated calcium may be important in the subsequent proliferative response.", "PMID": 956662}
{"id": "pubmed23n0032_2318", "title": "The primary structure of porcine C3a anaphylatoxin.", "content": "Porcine C3a was generated in whole porcine serum by inulin activation of enzymes of the alternative complement pathway. The C3a anaphylatoxin was isolated according to the procedures previously described by Hugli. The complete amino acid sequence for porcine C3a was determined utilizing automatic sequencing techniques in addition to manual subtractive Edman degradation and carboxypeptidase A, B, or Y digestion of isolated peptides. Porcine C3a is composed of a polypeptide chain containing 77 amino acid residues and has a m.w. of approximately 9,000 daltons. This C3a molecule is devoid of threonine, tryptophan, and carbohydrates. The proposed primary structure for porcine C3a is as follows: (see article) Comparisons between the amino acid sequences of human and porcine C3a reveal that the six half-cystinyl and five aromatic residue positions are conserved. Conservation of these six half-cystinyl residue positions suggest that the disulfide arrangement remains identical in both anaphylatoxin molecules. Maintenance of three interconnected disulfide linkages helps to explain a near identity between the secondary structures of human and porcine C3a as indicated by circular dichroism measurements. Particular attention was focused on the COOH-terminal region of the anaphylatoxins since an arginyl residue at position 77 is functionally essential in both human and porcine C3a. Five residue positions at the carboxy termini were conserved in both C3a molecules, and the sequence Leu-Gly-Leu-Ala-Arg probably relates directly to anaphylatoxin activity. A total of 23 residue replacements occur between human and porcine C3a which accounts for a 30% difference in primary structure. Although the C3a molecules exhibit identical biologic activity, this rather large structural difference readily explains the absence of a detectable immunologic cross-reactivity.", "contents": "The primary structure of porcine C3a anaphylatoxin. Porcine C3a was generated in whole porcine serum by inulin activation of enzymes of the alternative complement pathway. The C3a anaphylatoxin was isolated according to the procedures previously described by Hugli. The complete amino acid sequence for porcine C3a was determined utilizing automatic sequencing techniques in addition to manual subtractive Edman degradation and carboxypeptidase A, B, or Y digestion of isolated peptides. Porcine C3a is composed of a polypeptide chain containing 77 amino acid residues and has a m.w. of approximately 9,000 daltons. This C3a molecule is devoid of threonine, tryptophan, and carbohydrates. The proposed primary structure for porcine C3a is as follows: (see article) Comparisons between the amino acid sequences of human and porcine C3a reveal that the six half-cystinyl and five aromatic residue positions are conserved. Conservation of these six half-cystinyl residue positions suggest that the disulfide arrangement remains identical in both anaphylatoxin molecules. Maintenance of three interconnected disulfide linkages helps to explain a near identity between the secondary structures of human and porcine C3a as indicated by circular dichroism measurements. Particular attention was focused on the COOH-terminal region of the anaphylatoxins since an arginyl residue at position 77 is functionally essential in both human and porcine C3a. Five residue positions at the carboxy termini were conserved in both C3a molecules, and the sequence Leu-Gly-Leu-Ala-Arg probably relates directly to anaphylatoxin activity. A total of 23 residue replacements occur between human and porcine C3a which accounts for a 30% difference in primary structure. Although the C3a molecules exhibit identical biologic activity, this rather large structural difference readily explains the absence of a detectable immunologic cross-reactivity.", "PMID": 956663}
{"id": "pubmed23n0032_2319", "title": "Production and characterisation of antisera to diphenylhydantoin suitable for radioimmunoassay.", "content": "The effect of carrier protein, nature of hapten-carrier bridge, and density of hapten substitution on the immunogenicity of diphenylhydantoin (DPH) derivatives in rabbits is described. DPH-3-valerate-bovine serum albumin (BSA) with a hapten: protein ratio of 27: 1 yields antisera of high titre and specificity for DPH. An antiserum to DPH-valerate-BSA was employed to develop a rapid, sensitive, one stage double antibody radioimmunoassay suitable for clinical application to serum, saliva, and urine samples. The assay, using 14C-DPH as tracer, is accurate and precise over the range 0.5--50 mug DPH per ml. Results by radioimmunoassay correlate closely with those obtained by gas-liquid chromatography (r = 0.97).", "contents": "Production and characterisation of antisera to diphenylhydantoin suitable for radioimmunoassay. The effect of carrier protein, nature of hapten-carrier bridge, and density of hapten substitution on the immunogenicity of diphenylhydantoin (DPH) derivatives in rabbits is described. DPH-3-valerate-bovine serum albumin (BSA) with a hapten: protein ratio of 27: 1 yields antisera of high titre and specificity for DPH. An antiserum to DPH-valerate-BSA was employed to develop a rapid, sensitive, one stage double antibody radioimmunoassay suitable for clinical application to serum, saliva, and urine samples. The assay, using 14C-DPH as tracer, is accurate and precise over the range 0.5--50 mug DPH per ml. Results by radioimmunoassay correlate closely with those obtained by gas-liquid chromatography (r = 0.97).", "PMID": 956664}
{"id": "pubmed23n0032_2320", "title": "An evaluation of an assay for DNA synthesis in lymphocytes with [3H]thymidine and harvesting on to glass fibre filter discs.", "content": "Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 mjCi or [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.", "contents": "An evaluation of an assay for DNA synthesis in lymphocytes with [3H]thymidine and harvesting on to glass fibre filter discs. Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 mjCi or [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.", "PMID": 956665}
{"id": "pubmed23n0032_2321", "title": "Marihuana-induced antibody response.", "content": "Utilizing an indirect Coombs' technique, humoral antibodies to marihuana and its major components were found in 34 subjects with known marihuana exposure. Similar antibodies were absent in the sera of a control population of 34 subjects with no known marihuana exposure. The specificity of the antibodies was confirmed by differential absorption studies in six seropositive subjects. Routine laboratory tests were abnormal in 22 of 34 seropositive subjects and in 9 of 34 control subjects. The relationship of seropositivity to abnormal laboratory tests is not certain.", "contents": "Marihuana-induced antibody response. Utilizing an indirect Coombs' technique, humoral antibodies to marihuana and its major components were found in 34 subjects with known marihuana exposure. Similar antibodies were absent in the sera of a control population of 34 subjects with no known marihuana exposure. The specificity of the antibodies was confirmed by differential absorption studies in six seropositive subjects. Routine laboratory tests were abnormal in 22 of 34 seropositive subjects and in 9 of 34 control subjects. The relationship of seropositivity to abnormal laboratory tests is not certain.", "PMID": 956682}
{"id": "pubmed23n0032_2322", "title": "Interference by 2, 3-dimercapto-1-propanol (BAL) in angiotensin I radioimmunoassay.", "content": "The angiotensinase inhibitor 2,3-dimercaptopropanol (BAL) interferes with peptide-antibody binding when certain sensitive antisera are used in angiotensin I radioimmunoassay systems. Three of nine antisera tested showed sufficient interference to produce serious errors in data obtained using these antisera together with BAL. For PRA determinations in human plasma, at both pH 5.7 and pH 7.3, relationships between different PRA'S are altered, and results of renin stimulation tests are changed in unpredictable ways. Determination of renin concentration in rat plasma does not require use of BAL as inhibitor, and it is best avoided. For human plasma renin determinations, use of BAL-sensitive antisera should be avoided, since there is no satisfactory way to correct data for the resulting error. BAL itself, rather than its oxidation products, is probably the interfering substance. The interference appears to be due to an interaction between BAL and the BAL-sensitive antiserum. It is not related to the known actions of BAL as chelating or reducing agent.", "contents": "Interference by 2, 3-dimercapto-1-propanol (BAL) in angiotensin I radioimmunoassay. The angiotensinase inhibitor 2,3-dimercaptopropanol (BAL) interferes with peptide-antibody binding when certain sensitive antisera are used in angiotensin I radioimmunoassay systems. Three of nine antisera tested showed sufficient interference to produce serious errors in data obtained using these antisera together with BAL. For PRA determinations in human plasma, at both pH 5.7 and pH 7.3, relationships between different PRA'S are altered, and results of renin stimulation tests are changed in unpredictable ways. Determination of renin concentration in rat plasma does not require use of BAL as inhibitor, and it is best avoided. For human plasma renin determinations, use of BAL-sensitive antisera should be avoided, since there is no satisfactory way to correct data for the resulting error. BAL itself, rather than its oxidation products, is probably the interfering substance. The interference appears to be due to an interaction between BAL and the BAL-sensitive antiserum. It is not related to the known actions of BAL as chelating or reducing agent.", "PMID": 956683}
{"id": "pubmed23n0032_2323", "title": "Studies on platelet plasma membranes. II. Characterization of surface proteins of rabbit platelets in vitro and during circulation in vivo using diazotized (125i)-diiodosulfanilic acid as a label.", "content": "Diazotized (125I)-diiodosulfanilic acid (DD125ISA) binds specifically to the exposed proteins on the surface of the rabbit platelet plasma membrane. This was demonstrated by the following observations with the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole platelets and the isolated plasma membrane fraction: (1) the specific activity of isolated membrane protein was sevenfold that of whole platelet protein, (2) no proteins of intact platelets were labeled which were not represented in the isolated plasma membrane, (3) DD125ISA-labeled proteins were altered by trypsin treatment of intact, labeled platelets, and (4) the pattern of labeling produced by reaction of isolated membranes with DD125ISA differed from that produced by the labeling of intact platelets. Reaction of DD125ISA with intact platelets produced labeling of only the three membrane glycoproteins (molecular weights: 180,000, 125,000, and 92,000 daltons) with greatest labeling of the largest glycoprotein and least labeling of the smallest glycoprotein. When rabbit platelets were labeled simultaneously with DD125ISA and 51Cr, the two isotopes were similarly distributed in various density populations of platelets. Some DD125ISA was solubilized from labeled and washed platelets by sonication, but all platelet DD125ISA was recovered in the plasma membrane fraction after 30 minutes' circulation in vivo. In vivo 51Cr recovery and survival were not altered by simultaneous labeling of platelets with DD125ISA. The disappearance of DD125ISA from circulating platelets (T 1/2 = 17 hours) was more rapid than 51Cr (T 1/2 = 30 hours) and appeared exponential in contrast to the linear 51Cr disappearance. On the other hand, DD125ISA did not disappear from platelets faster than 51Cr when doubly labeled platelets were harvested after 3 hours' circulation and incubated in autologous plasma (T 1/2 of DD125ISA elution = 43 hours, 51Cr = 33 hours). SDS-PAGE analysis of DD125ISA-labeled platelets after 14 to 20 hours' circulation in vivo demonstrated the same pattern of DD125ISA distribution on membrane glycoproteins as on the platelets prior to infusion. We interpret this symmetrical loss of the membrane label to indicate symmetrical loss of membrane proteins, suggesting that the platelet may lose pieces of membrane and not specific surface proteins during circulation. This could occur during reversible adhesion encounters during the process of hemostasis and cause the smaller size and decreased effectiveness of older platelets.", "contents": "Studies on platelet plasma membranes. II. Characterization of surface proteins of rabbit platelets in vitro and during circulation in vivo using diazotized (125i)-diiodosulfanilic acid as a label. Diazotized (125I)-diiodosulfanilic acid (DD125ISA) binds specifically to the exposed proteins on the surface of the rabbit platelet plasma membrane. This was demonstrated by the following observations with the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole platelets and the isolated plasma membrane fraction: (1) the specific activity of isolated membrane protein was sevenfold that of whole platelet protein, (2) no proteins of intact platelets were labeled which were not represented in the isolated plasma membrane, (3) DD125ISA-labeled proteins were altered by trypsin treatment of intact, labeled platelets, and (4) the pattern of labeling produced by reaction of isolated membranes with DD125ISA differed from that produced by the labeling of intact platelets. Reaction of DD125ISA with intact platelets produced labeling of only the three membrane glycoproteins (molecular weights: 180,000, 125,000, and 92,000 daltons) with greatest labeling of the largest glycoprotein and least labeling of the smallest glycoprotein. When rabbit platelets were labeled simultaneously with DD125ISA and 51Cr, the two isotopes were similarly distributed in various density populations of platelets. Some DD125ISA was solubilized from labeled and washed platelets by sonication, but all platelet DD125ISA was recovered in the plasma membrane fraction after 30 minutes' circulation in vivo. In vivo 51Cr recovery and survival were not altered by simultaneous labeling of platelets with DD125ISA. The disappearance of DD125ISA from circulating platelets (T 1/2 = 17 hours) was more rapid than 51Cr (T 1/2 = 30 hours) and appeared exponential in contrast to the linear 51Cr disappearance. On the other hand, DD125ISA did not disappear from platelets faster than 51Cr when doubly labeled platelets were harvested after 3 hours' circulation and incubated in autologous plasma (T 1/2 of DD125ISA elution = 43 hours, 51Cr = 33 hours). SDS-PAGE analysis of DD125ISA-labeled platelets after 14 to 20 hours' circulation in vivo demonstrated the same pattern of DD125ISA distribution on membrane glycoproteins as on the platelets prior to infusion. We interpret this symmetrical loss of the membrane label to indicate symmetrical loss of membrane proteins, suggesting that the platelet may lose pieces of membrane and not specific surface proteins during circulation. This could occur during reversible adhesion encounters during the process of hemostasis and cause the smaller size and decreased effectiveness of older platelets.", "PMID": 956684}
{"id": "pubmed23n0032_2324", "title": "Sarcoidosis with salivary gland involvement: biochemical studies on parotid saliva.", "content": "Parotid saliva from a patient suffering from sarcoidosis with salivary gland involvement has been shown to have a decreased level of alpha-amylase but increased levels of albumin and lysozyme. These observations suggest that in addition to impaired gland function, gland damage as a result of inflammation had occurred which permitted increased passage of constituents from serum into the gland secretion.", "contents": "Sarcoidosis with salivary gland involvement: biochemical studies on parotid saliva. Parotid saliva from a patient suffering from sarcoidosis with salivary gland involvement has been shown to have a decreased level of alpha-amylase but increased levels of albumin and lysozyme. These observations suggest that in addition to impaired gland function, gland damage as a result of inflammation had occurred which permitted increased passage of constituents from serum into the gland secretion.", "PMID": 956685}
{"id": "pubmed23n0032_2325", "title": "Halofenate: a potent inhibitor of normal and hypersensitive platelets.", "content": "Platelet hypersensitivity has been documented in diabetes and angina pectoris and can be partially reversed in hyperbetalipoproteinemia by clofibrate. We therefore examined the effects of incubating another lipid-lowering agent, halofenate, with both normal platelets and platelets made hypersensitive in vitro by incorporation of 55 per cent excess cholesterol into their membranes. At therapeutic concentrations, halofenate caused a time- and dose-dependent inhibition of the aggregation of normal platelets by epinephrine. After 30 minutes' incubation at 37 degrees C., halofenate significantly inhibited the extent of aggregation by 88 per cent (p less than 0.01), whereas clofibrate inhibited aggregation by 44 per cent (p less than 0.01). Halofenate was a more potent inhibitor of platelets than clofibrate (p less than 0.01). The mean threshold concentration of epinephrine necessary for aggregation of normal platelets (4.2 muM) was not significatnly increased with clofibrate (10 muM) but was markedly elevated with halofenate (245 muM; p less than 0.001). Significant but less dramatic increases in threshold concentration of ADP and collagen were found with halofenate but no clofibrate. Cholesterol-rich platelets were 114-fold more sensitive to epinephrine and twofold more sensitive to ADP than normal platelets but after incubation with halofenate became even less sensitive than normal. Clofibrate inhibited the extent of aggregation of hypersensitive platelets but did not alter the threshold concentration of epinephrine necessary for aggregation. Thus, halofenate is more potent than clofibrate in reducing the sensitivity of normal platelets to aggregating agents in vitro and can completely reverse experimentally produced platelet hypersensitivity. These data suggest that halofenate might be useful in reversing increased platelet sensitivity in cardiovascular diseases.", "contents": "Halofenate: a potent inhibitor of normal and hypersensitive platelets. Platelet hypersensitivity has been documented in diabetes and angina pectoris and can be partially reversed in hyperbetalipoproteinemia by clofibrate. We therefore examined the effects of incubating another lipid-lowering agent, halofenate, with both normal platelets and platelets made hypersensitive in vitro by incorporation of 55 per cent excess cholesterol into their membranes. At therapeutic concentrations, halofenate caused a time- and dose-dependent inhibition of the aggregation of normal platelets by epinephrine. After 30 minutes' incubation at 37 degrees C., halofenate significantly inhibited the extent of aggregation by 88 per cent (p less than 0.01), whereas clofibrate inhibited aggregation by 44 per cent (p less than 0.01). Halofenate was a more potent inhibitor of platelets than clofibrate (p less than 0.01). The mean threshold concentration of epinephrine necessary for aggregation of normal platelets (4.2 muM) was not significatnly increased with clofibrate (10 muM) but was markedly elevated with halofenate (245 muM; p less than 0.001). Significant but less dramatic increases in threshold concentration of ADP and collagen were found with halofenate but no clofibrate. Cholesterol-rich platelets were 114-fold more sensitive to epinephrine and twofold more sensitive to ADP than normal platelets but after incubation with halofenate became even less sensitive than normal. Clofibrate inhibited the extent of aggregation of hypersensitive platelets but did not alter the threshold concentration of epinephrine necessary for aggregation. Thus, halofenate is more potent than clofibrate in reducing the sensitivity of normal platelets to aggregating agents in vitro and can completely reverse experimentally produced platelet hypersensitivity. These data suggest that halofenate might be useful in reversing increased platelet sensitivity in cardiovascular diseases.", "PMID": 956686}
{"id": "pubmed23n0032_2326", "title": "Quantitation of maximal bactericidal capability in human neutrophils.", "content": "The quantitative capacity of normal human neutrophils to ingest and kill increasing multiples of bacteria up to a level of saturation was evaluated. The in vitro system, a modification of classic bactericidal assay, employed carefully controlled ratios of bacteria per neutrophil between 1.25 and 400:1 to stress neutrophil function. Morphologic examination and measurement of bacterial survival by quantitative plate culturing indicated that at a challenge of about 100 Staphylococcus aureus per neutrophil bactericidal activity reached its peak with each average cell killing 48 bacteria. The efficiency of bactericidal function fell as the challenge was increased while ingestion capabilities kept pace with the numbers of bacteria presented to the neutrophils. Various key test parameters were evaluated from which evolved a system which provides a highly reproducible, statistically based, method for clinical assessment of subtle or partial neutrophil dysfunctions which may escape detection by the currently employed techniques of bactericidal assay.", "contents": "Quantitation of maximal bactericidal capability in human neutrophils. The quantitative capacity of normal human neutrophils to ingest and kill increasing multiples of bacteria up to a level of saturation was evaluated. The in vitro system, a modification of classic bactericidal assay, employed carefully controlled ratios of bacteria per neutrophil between 1.25 and 400:1 to stress neutrophil function. Morphologic examination and measurement of bacterial survival by quantitative plate culturing indicated that at a challenge of about 100 Staphylococcus aureus per neutrophil bactericidal activity reached its peak with each average cell killing 48 bacteria. The efficiency of bactericidal function fell as the challenge was increased while ingestion capabilities kept pace with the numbers of bacteria presented to the neutrophils. Various key test parameters were evaluated from which evolved a system which provides a highly reproducible, statistically based, method for clinical assessment of subtle or partial neutrophil dysfunctions which may escape detection by the currently employed techniques of bactericidal assay.", "PMID": 956687}
{"id": "pubmed23n0032_2327", "title": "The removal of leukocytes and platelets from whole blood.", "content": "Various methods for the removal of leukocytes from whole blood have been compared and a new technique has been devised. This procedure consists of passing whole blood through a bed composed of microcrystalline cellulose and alpha-cellulose. The method is rapid, reliable, removes over 99.75 per cent of the leukocytes from blood, and does not seem selectively to retain reticulocytes or to release a significant proportion of leukocyte enzymes. Most of the platelets are also removed from anticoagulated blood, and platelet-free red cells can be obtained by passing defibrinated blood over the microcrystalline cellulose-alpha-cellulose bed.", "contents": "The removal of leukocytes and platelets from whole blood. Various methods for the removal of leukocytes from whole blood have been compared and a new technique has been devised. This procedure consists of passing whole blood through a bed composed of microcrystalline cellulose and alpha-cellulose. The method is rapid, reliable, removes over 99.75 per cent of the leukocytes from blood, and does not seem selectively to retain reticulocytes or to release a significant proportion of leukocyte enzymes. Most of the platelets are also removed from anticoagulated blood, and platelet-free red cells can be obtained by passing defibrinated blood over the microcrystalline cellulose-alpha-cellulose bed.", "PMID": 956688}
{"id": "pubmed23n0032_2328", "title": "Lipid, glucose, and insulin interrelationships in normal, prediabetic, and chemical diabetic subjects.", "content": "Fasting levels of plasma cholersterol and triglyceride, lipoprotein patterns, and fasting and postintravenous glucose levels of blood glucose, serum insulin, serum growth hormone, and plasma free fatty acids in a genetic potential diabetic population were compared to those in a similar normal control population. THe potential diabetic population was further divided into groups of patients with a normal (prediabetic) or abnormal (chemical diabetic) glucose tolerance test. Although no clear-cut lipid differences were noted, certain trends appeared. More type IV hyperlipoproteinemia was seen in male prediabetic (21%) and male chemical diabetic patients (19%) than in normal male subjects (5%); in female subjects only a few type IV patterns were seen. Type II hyperlipoproteinemia was not seen in any normal subject, but was noted in nearly 9% of those with chemical diabetes. Fasting cholesterol levels correlated better with age than did fasting triglyceride levels in most of the patient groups. Fasting triglyceride levels showed a significant positive correlation with the serum insulin area of the oral glucose tolerance test in the normals wna prediabetic persons, and also showed a significant positive correlation with the blood glucose area of the prediabetic and chemical diabetic patients. It is suggested that a normal relationship between triglyceride concentration and insulin response to glucose is lost in chemical diabetes. Sex differences were also noted in the inslin response and the insulin-glucose relationships during the oral glucose tolerance test, with normal menstruation women showing a significantly lower insulin-glucose relationship than the age-related men.", "contents": "Lipid, glucose, and insulin interrelationships in normal, prediabetic, and chemical diabetic subjects. Fasting levels of plasma cholersterol and triglyceride, lipoprotein patterns, and fasting and postintravenous glucose levels of blood glucose, serum insulin, serum growth hormone, and plasma free fatty acids in a genetic potential diabetic population were compared to those in a similar normal control population. THe potential diabetic population was further divided into groups of patients with a normal (prediabetic) or abnormal (chemical diabetic) glucose tolerance test. Although no clear-cut lipid differences were noted, certain trends appeared. More type IV hyperlipoproteinemia was seen in male prediabetic (21%) and male chemical diabetic patients (19%) than in normal male subjects (5%); in female subjects only a few type IV patterns were seen. Type II hyperlipoproteinemia was not seen in any normal subject, but was noted in nearly 9% of those with chemical diabetes. Fasting cholesterol levels correlated better with age than did fasting triglyceride levels in most of the patient groups. Fasting triglyceride levels showed a significant positive correlation with the serum insulin area of the oral glucose tolerance test in the normals wna prediabetic persons, and also showed a significant positive correlation with the blood glucose area of the prediabetic and chemical diabetic patients. It is suggested that a normal relationship between triglyceride concentration and insulin response to glucose is lost in chemical diabetes. Sex differences were also noted in the inslin response and the insulin-glucose relationships during the oral glucose tolerance test, with normal menstruation women showing a significantly lower insulin-glucose relationship than the age-related men.", "PMID": 956689}
{"id": "pubmed23n0032_2329", "title": "The effect of age on plasma proinsulin-like material after oral glucose.", "content": "The total plasma insulin and proinsulin-like material after oral glucose was studied in 68 lean subjects of varying ages with normal glucose tolerance tests. Although each subject had a normal test, the mean glucose levels increased with increasing age. When younger subjects (ages 15 to 44) were compared with older (45 to 74), no significant differences in total insulin responses were seen, but plasma proinsulin-like material was significantly higher in the older age subjects. All values after stimulation were significantly greater in subjects 45 to 74 years of age than in those 15 to 44  years of age (p less than 0.01). A significant correlation between the amount of proinsulin and the age of the subject was seen. These findings may reflect a decreased conversion of proinsulin to insulin in the aging pancreas or could reflect decreased clearance of proinsulin in older subjects.", "contents": "The effect of age on plasma proinsulin-like material after oral glucose. The total plasma insulin and proinsulin-like material after oral glucose was studied in 68 lean subjects of varying ages with normal glucose tolerance tests. Although each subject had a normal test, the mean glucose levels increased with increasing age. When younger subjects (ages 15 to 44) were compared with older (45 to 74), no significant differences in total insulin responses were seen, but plasma proinsulin-like material was significantly higher in the older age subjects. All values after stimulation were significantly greater in subjects 45 to 74 years of age than in those 15 to 44  years of age (p less than 0.01). A significant correlation between the amount of proinsulin and the age of the subject was seen. These findings may reflect a decreased conversion of proinsulin to insulin in the aging pancreas or could reflect decreased clearance of proinsulin in older subjects.", "PMID": 956690}
{"id": "pubmed23n0032_2330", "title": "Human vs. dog platelet adhesion to Cuprophane under controlled conditions of whole blood flow.", "content": "It is commonly assumed that a candidate biomaterial to which nonhuman (especially dog) platelets do not readily adhere is promising for human use. This assumption has been tested in a lucite chamber in which Cuprophane membrane (Bemberg PT-150), comprising one of the chamber surfaces, is exposed to citrated or heparinized flowing dog or human blood for 10 or 20 minutes and flowing buffer for 1 minute. Following exposure, membrane specimens are carefully removed, fixed in glutaraldehyde, and mounted on a slide. Platelets at a particular axial location are counted by phase-contrast microscopy (625 X). At a shear rate of 986 sec.-1 and at 10 minutes, platelets per square millimeter (mean +/- S.E.(N) in citrate were 28,400 +/- 5,000 (6), dog; 18 +/- 3.8 (4), human. Under the same conditions, platelets per squar millimeter in heparin were 32,600 +/- 8,400 (4), dog; 7.6 +/- 1.0 (3), human. A qualitatively similar species difference was found in citrate at one shear rate and at one other exposure time. Those observations suggest that evaluations of candidate human biomaterials carried out with dog (and perhaps other nonhuman) blood must be interpreted with caution.", "contents": "Human vs. dog platelet adhesion to Cuprophane under controlled conditions of whole blood flow. It is commonly assumed that a candidate biomaterial to which nonhuman (especially dog) platelets do not readily adhere is promising for human use. This assumption has been tested in a lucite chamber in which Cuprophane membrane (Bemberg PT-150), comprising one of the chamber surfaces, is exposed to citrated or heparinized flowing dog or human blood for 10 or 20 minutes and flowing buffer for 1 minute. Following exposure, membrane specimens are carefully removed, fixed in glutaraldehyde, and mounted on a slide. Platelets at a particular axial location are counted by phase-contrast microscopy (625 X). At a shear rate of 986 sec.-1 and at 10 minutes, platelets per square millimeter (mean +/- S.E.(N) in citrate were 28,400 +/- 5,000 (6), dog; 18 +/- 3.8 (4), human. Under the same conditions, platelets per squar millimeter in heparin were 32,600 +/- 8,400 (4), dog; 7.6 +/- 1.0 (3), human. A qualitatively similar species difference was found in citrate at one shear rate and at one other exposure time. Those observations suggest that evaluations of candidate human biomaterials carried out with dog (and perhaps other nonhuman) blood must be interpreted with caution.", "PMID": 956691}
{"id": "pubmed23n0032_2331", "title": "Renal function in experimental cystic disease of the rat.", "content": "The administration of diphenylamine to rats induces an acquired form of cystic disease. In order to examine the early changes in this model of experimental cystic disease prior to the development of the more severe structural alterations, clearance, micropuncture, and morphologic studies were performed in rats fed DPA for 3 to 6 weeks. A significant defect in maximal urine concentrating ability (Umax) was manifest by the second week and averaged 50% of control values. Further studies were undertaken to examine the cause of the defect in Umax. Whole-kidney glomerular filtration rate (GFR), single-nephron GFR, end-proximal TF/Pinulin, glucose and bicarbonate reabsorption were all normal, indicating normal function of the proximal tubule. Free water clearance and free water reabsorption were not significantly different in DPA-treated rats as compared to controls, suggesting normal function of the ascending limb of the loop of Henle and collecting duct. Morphologic examination revealed gross cysts in less than 10% of the kidneys but structural changes were consistently demonstrated in the collecting ducts of DPA-treated rats. These studies indicate that the decrease in Umax in DPA-treated animals is the result of a defect located at the terminal portion of the collecting duct.", "contents": "Renal function in experimental cystic disease of the rat. The administration of diphenylamine to rats induces an acquired form of cystic disease. In order to examine the early changes in this model of experimental cystic disease prior to the development of the more severe structural alterations, clearance, micropuncture, and morphologic studies were performed in rats fed DPA for 3 to 6 weeks. A significant defect in maximal urine concentrating ability (Umax) was manifest by the second week and averaged 50% of control values. Further studies were undertaken to examine the cause of the defect in Umax. Whole-kidney glomerular filtration rate (GFR), single-nephron GFR, end-proximal TF/Pinulin, glucose and bicarbonate reabsorption were all normal, indicating normal function of the proximal tubule. Free water clearance and free water reabsorption were not significantly different in DPA-treated rats as compared to controls, suggesting normal function of the ascending limb of the loop of Henle and collecting duct. Morphologic examination revealed gross cysts in less than 10% of the kidneys but structural changes were consistently demonstrated in the collecting ducts of DPA-treated rats. These studies indicate that the decrease in Umax in DPA-treated animals is the result of a defect located at the terminal portion of the collecting duct.", "PMID": 956692}
{"id": "pubmed23n0032_2332", "title": "Platelets are not essential for the pulmonary vascular pressor response to hypoxia.", "content": "The literature suggests that platelets might help mediate the pulmonary vascular pressor response to hypoxia. This study evaluated the hypoxic response in thrombocytopenic dogs. Platet depletion was achieved in five dogs by the use of platelet antiserum. In the normoxic state these dogs had lower cardiac outputs and higher pulmonary and systemic vascular resistances than five control dogs. The pressor response to hypoxia in these dogs was not only preserved but considerably enhanced in comparison to the control dogs. Hypoxia increased the pulmonary vascular resistance 146 +/- 17% above its normoxic value in the thrombocytopenic dogs and 64 +/- 21% in the control dogs. Thus platelets may normally produce a dilator substance or inactivate a pressor substance during hypoxia. The mechanism of the effect is not apparent but it is clear tha the pulmonary pressor response to hypoxia in the dog is not mediated by platelets.", "contents": "Platelets are not essential for the pulmonary vascular pressor response to hypoxia. The literature suggests that platelets might help mediate the pulmonary vascular pressor response to hypoxia. This study evaluated the hypoxic response in thrombocytopenic dogs. Platet depletion was achieved in five dogs by the use of platelet antiserum. In the normoxic state these dogs had lower cardiac outputs and higher pulmonary and systemic vascular resistances than five control dogs. The pressor response to hypoxia in these dogs was not only preserved but considerably enhanced in comparison to the control dogs. Hypoxia increased the pulmonary vascular resistance 146 +/- 17% above its normoxic value in the thrombocytopenic dogs and 64 +/- 21% in the control dogs. Thus platelets may normally produce a dilator substance or inactivate a pressor substance during hypoxia. The mechanism of the effect is not apparent but it is clear tha the pulmonary pressor response to hypoxia in the dog is not mediated by platelets.", "PMID": 956693}
{"id": "pubmed23n0032_2333", "title": "Selective diphenylhydantoin-induced suppression of lymphocyte reactivity in vitro.", "content": "Diphenylhydantoin (DPH) in concentrations approximating therapeutic blood levels was found to inhibit mixed lymphocyte culture (MLC) proliferation as measured by uptake of 3H-thymidine when compared to alkaline diluent required to solubilize the DPH. Control cultures containing diluent alone manifested inhibition of 3H-thymidine uptake relative to non-diluent-containing cultures. Both reactor and stimulator cells were independently capable of effecting this inhibition after treatment with DPH. An inconsistent effect of DPH on lymphocyte reactivity to graded doses of phytohemagglutinin and to pokeweed mitogen was observed. A possible association between the effect of DPH on MLC reactivity and the phenomenon of dilantin-induced lymphadenopathy is suggested.", "contents": "Selective diphenylhydantoin-induced suppression of lymphocyte reactivity in vitro. Diphenylhydantoin (DPH) in concentrations approximating therapeutic blood levels was found to inhibit mixed lymphocyte culture (MLC) proliferation as measured by uptake of 3H-thymidine when compared to alkaline diluent required to solubilize the DPH. Control cultures containing diluent alone manifested inhibition of 3H-thymidine uptake relative to non-diluent-containing cultures. Both reactor and stimulator cells were independently capable of effecting this inhibition after treatment with DPH. An inconsistent effect of DPH on lymphocyte reactivity to graded doses of phytohemagglutinin and to pokeweed mitogen was observed. A possible association between the effect of DPH on MLC reactivity and the phenomenon of dilantin-induced lymphadenopathy is suggested.", "PMID": 956694}
{"id": "pubmed23n0032_2334", "title": "Hypercatabolism of the third component of complement in patients with primary biliary cirrhosis.", "content": "The possibliity that the disturbed immunity associated with primary biliary cirrhosis (PBC) includes abnormal metabolism of the complement system has been investigated by conducting simultaneous studies of the turnover of highly purified, hemolytically active, 125-i-labeled C3 (third component of complement) and 131i-albumin in seven control subjects and nine patients with PBC. Aliquots of each 125i-C3 preparation were injected, together with 131i-albumin, into one or two normal subjects and one to three patients with PBC and plasma and urine radioactivity data were analyzed. In all patients with PBC disappearance of 125i from plasma was much more rapid than in controls. In five of these patients no terminal monexponential decline of 125i occurred. The mean fractional catabolic rate (FCR) of C3 was higher in PBC (4.23 +/- 0.32 [S.E.M.] per cent IV pool per hour) than in controls (2.02 +/- 0.08% IV pool per hour, p less than 0.0005). In contrast, the mean FCR of albumin was similar in PBC (11.4 +/- 1.29 per cent IV pool per day) and controls (11.5 +/- 0.76 per cent IV pool per day), suggesting that the increased FCR of C3 in PBC was not due to a nonspecific process. There was no correlation between values for the FCR of C3 and indices of cholestasis. The mean synthetic rate of C3 was higher in PBC (2.94 +/- 0.48 MG. PER KILOGRAM PER HOUR) THAN IN CONTROls (1.03 +/- 0.11 mg. per kilogram per hour, p less than 0.0025). In five patients relatively more C3 was extravascular than in controls. The results are consistent with PBC being associated with chronic activation of the complement system and increased tissue attachement of C3, phenomena which could be related to a process of pathogenic significance in this disease.", "contents": "Hypercatabolism of the third component of complement in patients with primary biliary cirrhosis. The possibliity that the disturbed immunity associated with primary biliary cirrhosis (PBC) includes abnormal metabolism of the complement system has been investigated by conducting simultaneous studies of the turnover of highly purified, hemolytically active, 125-i-labeled C3 (third component of complement) and 131i-albumin in seven control subjects and nine patients with PBC. Aliquots of each 125i-C3 preparation were injected, together with 131i-albumin, into one or two normal subjects and one to three patients with PBC and plasma and urine radioactivity data were analyzed. In all patients with PBC disappearance of 125i from plasma was much more rapid than in controls. In five of these patients no terminal monexponential decline of 125i occurred. The mean fractional catabolic rate (FCR) of C3 was higher in PBC (4.23 +/- 0.32 [S.E.M.] per cent IV pool per hour) than in controls (2.02 +/- 0.08% IV pool per hour, p less than 0.0005). In contrast, the mean FCR of albumin was similar in PBC (11.4 +/- 1.29 per cent IV pool per day) and controls (11.5 +/- 0.76 per cent IV pool per day), suggesting that the increased FCR of C3 in PBC was not due to a nonspecific process. There was no correlation between values for the FCR of C3 and indices of cholestasis. The mean synthetic rate of C3 was higher in PBC (2.94 +/- 0.48 MG. PER KILOGRAM PER HOUR) THAN IN CONTROls (1.03 +/- 0.11 mg. per kilogram per hour, p less than 0.0025). In five patients relatively more C3 was extravascular than in controls. The results are consistent with PBC being associated with chronic activation of the complement system and increased tissue attachement of C3, phenomena which could be related to a process of pathogenic significance in this disease.", "PMID": 956695}
{"id": "pubmed23n0032_2335", "title": "Hemostatic alterations accompanying sickle cell pain crises.", "content": "Three patients with sickle-cell disease (SSD) were followed, weekly, for 1 1/2 to 2 years, during which time they experienced one or more episodes of crisis. Crisis was associated with reproducible sequential hemostatic alterations indicating intravascular fibrin formation and a marked disturbance in platelet economy. With crisis onset, or possibly before, there was an increase in plasma high-molecular-weight fibrinogen complexes and a transient fall in platelet count, with a subsequent rise in both fibrinogen concentration and platelet count; plasma fibrinogen peaked 1 week after crisis onset and platelet count approximately 2 weeks after onset. Subsidence of crisis was associated with a fall in high-molecular-weight fibrinogen complexes and a subsequent increase in fibrinogen first derivative, an early fibrinogen breakdown product. Hemostatic findings and patient clinical status were generally correlated, the findings during asymptomatic periods being essentially normal. Agents affecting platelet function (aspirin alone or in combination with dipyridamole) appeared to reduce the extent of laboratory abnormality, suggesting potential clinical usefulness in this disorder.", "contents": "Hemostatic alterations accompanying sickle cell pain crises. Three patients with sickle-cell disease (SSD) were followed, weekly, for 1 1/2 to 2 years, during which time they experienced one or more episodes of crisis. Crisis was associated with reproducible sequential hemostatic alterations indicating intravascular fibrin formation and a marked disturbance in platelet economy. With crisis onset, or possibly before, there was an increase in plasma high-molecular-weight fibrinogen complexes and a transient fall in platelet count, with a subsequent rise in both fibrinogen concentration and platelet count; plasma fibrinogen peaked 1 week after crisis onset and platelet count approximately 2 weeks after onset. Subsidence of crisis was associated with a fall in high-molecular-weight fibrinogen complexes and a subsequent increase in fibrinogen first derivative, an early fibrinogen breakdown product. Hemostatic findings and patient clinical status were generally correlated, the findings during asymptomatic periods being essentially normal. Agents affecting platelet function (aspirin alone or in combination with dipyridamole) appeared to reduce the extent of laboratory abnormality, suggesting potential clinical usefulness in this disorder.", "PMID": 956696}
{"id": "pubmed23n0032_2336", "title": "The in vitro effect of primaquine on red cell phospholipid metabolism.", "content": "Primaquine converts erythrocytes into osmotically fragile spherocytes. Since primaquine increased the rate of acylation of erythrocyte lysophosphatidylcholine (LPC), it has been suggested that a deficiency of LPC is responsible for its hemolytic action. We have examined the validity of this hypothesis. In the absence of added lysophosphatides, primaquine slightly decreased the incorporation of albumin-bound palmitic acid-14C into phosphatidylcholine (PC) whereas that into phosphatidylethanolamine (PE) was more depressed. When unbound dispersions of fatty acid were used, red cells exposed to primaquine manifested a slight increase in palmitic acid-14C incorporation into PC whereas that into PE remained decreased. In the presence of exogenous LPC, control cells increased their incorporation of bound palmitic acid-14C into PC while decreasing it into PE. In these same experiments, red cells incubated with primaquine demonstrated a much greater enhancement of LPC acylation than control cells. Lysophosphatidylethanolamine acylation, while depressed, was still slightly greater than that of cells incubated with drug alone. The concentration of LPC in control and primaquine-treated erythrocytes incubated with exogenous LPC was comparable and was approximately five times that of cells not incubated with LPC. The osmotic fragility of erythrocytes correlated poorly with their concentration of LPC. The effect of primaquine on the incorporation of bound palmitic acid into erythrocyte PC was influenced by exogenous LPC with the reaction markedly increased in its presence but slightly depressed in its absence. The proposal that the hemolytic action of primaquine is due to a decrease in red cell LPC is not supported by the data.", "contents": "The in vitro effect of primaquine on red cell phospholipid metabolism. Primaquine converts erythrocytes into osmotically fragile spherocytes. Since primaquine increased the rate of acylation of erythrocyte lysophosphatidylcholine (LPC), it has been suggested that a deficiency of LPC is responsible for its hemolytic action. We have examined the validity of this hypothesis. In the absence of added lysophosphatides, primaquine slightly decreased the incorporation of albumin-bound palmitic acid-14C into phosphatidylcholine (PC) whereas that into phosphatidylethanolamine (PE) was more depressed. When unbound dispersions of fatty acid were used, red cells exposed to primaquine manifested a slight increase in palmitic acid-14C incorporation into PC whereas that into PE remained decreased. In the presence of exogenous LPC, control cells increased their incorporation of bound palmitic acid-14C into PC while decreasing it into PE. In these same experiments, red cells incubated with primaquine demonstrated a much greater enhancement of LPC acylation than control cells. Lysophosphatidylethanolamine acylation, while depressed, was still slightly greater than that of cells incubated with drug alone. The concentration of LPC in control and primaquine-treated erythrocytes incubated with exogenous LPC was comparable and was approximately five times that of cells not incubated with LPC. The osmotic fragility of erythrocytes correlated poorly with their concentration of LPC. The effect of primaquine on the incorporation of bound palmitic acid into erythrocyte PC was influenced by exogenous LPC with the reaction markedly increased in its presence but slightly depressed in its absence. The proposal that the hemolytic action of primaquine is due to a decrease in red cell LPC is not supported by the data.", "PMID": 956697}
{"id": "pubmed23n0032_2337", "title": "Plasma alpha-L-fucosidase: presence of a low activity variant in some normal individuals.", "content": "The expression of a variant alpha-L-fucosidase has been determined in both plasma and leukocytes. This variant enzyme is characterized by unusually tow actuvity in plasma, an altered pH activity profile, and increased heat lability. Individuals possessing this low activity variant in plasma have usual levels of alpha-L-fucosidase activity in leukocytes. The leukocyte enzyme also shows increased heat lability and has an altered pH activity profile. Gel filtration of plasma alpha-L-fucosidase revealed both a high molecular weight form, alpha-L-fucosidase I, and a low molecular weight form, alpha-L-fucosidase II. Only alpha-L-fucosidase II could be detected upon gel filtration of the variant enzyme from plasma. Both control and variant leukocyte alpha-L-fucosidase revealed two peaks by gel filtration but the proportion of alpha-L-fucosidase I was reduced in the variant enzyme sample. Alpha-L-fucosidase II from both control and variant plasma and leukocyte samples was heat labile and possessed little activity at pH 4.0 Alpha-L-fucosidase I from control plasma and leukocytes was more stable to heating and possessed more activity at pH 4.0 than alpha-L-fucosidase II. In contrast, alpha-L-fucosidase I from the variant leukocyte sample was heat labile and had little activity at pH 4.0 thus resembling alpha-L-fucosidase II. These results suggest that the variant is the consequence of an altered allele which affects alpha-L-fucosidase expression in all tissues.", "contents": "Plasma alpha-L-fucosidase: presence of a low activity variant in some normal individuals. The expression of a variant alpha-L-fucosidase has been determined in both plasma and leukocytes. This variant enzyme is characterized by unusually tow actuvity in plasma, an altered pH activity profile, and increased heat lability. Individuals possessing this low activity variant in plasma have usual levels of alpha-L-fucosidase activity in leukocytes. The leukocyte enzyme also shows increased heat lability and has an altered pH activity profile. Gel filtration of plasma alpha-L-fucosidase revealed both a high molecular weight form, alpha-L-fucosidase I, and a low molecular weight form, alpha-L-fucosidase II. Only alpha-L-fucosidase II could be detected upon gel filtration of the variant enzyme from plasma. Both control and variant leukocyte alpha-L-fucosidase revealed two peaks by gel filtration but the proportion of alpha-L-fucosidase I was reduced in the variant enzyme sample. Alpha-L-fucosidase II from both control and variant plasma and leukocyte samples was heat labile and possessed little activity at pH 4.0 Alpha-L-fucosidase I from control plasma and leukocytes was more stable to heating and possessed more activity at pH 4.0 than alpha-L-fucosidase II. In contrast, alpha-L-fucosidase I from the variant leukocyte sample was heat labile and had little activity at pH 4.0 thus resembling alpha-L-fucosidase II. These results suggest that the variant is the consequence of an altered allele which affects alpha-L-fucosidase expression in all tissues.", "PMID": 956698}
{"id": "pubmed23n0032_2338", "title": "Approaches to the standardization of serum unsaturated iron-binding capacity.", "content": "An important aspect in the development and use of serum unsaturated iron-binding capacity (UIBC) procedures is the standardization of reference serum. Spectrophotometric titration of serum transferrin is the oldest of the UIBC methods; however, the literature on this subject indicates that the technique is not being fully exploited and describes a variety of suboptimal conditions. A spectrophotometric titration protocol is described which gives excellent reproducibility with fresh, frozen, lyophilized, and stored serum samples. An indirect UIBC method utilizing differential chelation and ultrafiltration is also described. In this method Fe3+ -NTA (nitrilotriacetic acid), in excess of the anticipated UIBC, is added to a serum sample supplemented with NaHC03. The addition of EDTA (ethelenediaminetetraacetic acid) allows the mobilization of iron from nonspecific macromolecular sites. The ultrafiltrate is analyzed for iron, and the UIBC is calculated. The results were validated via an ultrafiltration titration which was compared with a spectrophotometric titration. The precision of this method is excellent.", "contents": "Approaches to the standardization of serum unsaturated iron-binding capacity. An important aspect in the development and use of serum unsaturated iron-binding capacity (UIBC) procedures is the standardization of reference serum. Spectrophotometric titration of serum transferrin is the oldest of the UIBC methods; however, the literature on this subject indicates that the technique is not being fully exploited and describes a variety of suboptimal conditions. A spectrophotometric titration protocol is described which gives excellent reproducibility with fresh, frozen, lyophilized, and stored serum samples. An indirect UIBC method utilizing differential chelation and ultrafiltration is also described. In this method Fe3+ -NTA (nitrilotriacetic acid), in excess of the anticipated UIBC, is added to a serum sample supplemented with NaHC03. The addition of EDTA (ethelenediaminetetraacetic acid) allows the mobilization of iron from nonspecific macromolecular sites. The ultrafiltrate is analyzed for iron, and the UIBC is calculated. The results were validated via an ultrafiltration titration which was compared with a spectrophotometric titration. The precision of this method is excellent.", "PMID": 956699}
{"id": "pubmed23n0032_2339", "title": "A computer-based method for the quantification of leukocyte chemotaxis.", "content": "A computerized method for the counting of polymorphonuclear leukocytes (PMN's) on chemotactic membranes is described. Through the use of an optical scanner-computer accurate objective quantification is obtained which is similar to the results generated by visual tabulation of the same membranes. Futhermore, the electronic quantification method is both less time consuming and highly reproducible.", "contents": "A computer-based method for the quantification of leukocyte chemotaxis. A computerized method for the counting of polymorphonuclear leukocytes (PMN's) on chemotactic membranes is described. Through the use of an optical scanner-computer accurate objective quantification is obtained which is similar to the results generated by visual tabulation of the same membranes. Futhermore, the electronic quantification method is both less time consuming and highly reproducible.", "PMID": 956700}
{"id": "pubmed23n0032_2340", "title": "Radioimmunoassay of human Hageman factor (factor XII).", "content": "A secific, sensitive, and reproducible radioimmunoassay for human Hageman factor (HF, factory XII) has been developed with purified human HF and monospecific rabbit antibody. Precise measurements of HF antigen were possible for concentrations as low as 0.1% of that in normal pooled plasma. A good correlation (correlation co-efficient = 0.82) existed between the titers of HF measured by clot-promoting assays and radioimmunoassays among 42 normal adults. Confirming earlier studies, HF antigen was absent in Hageman trait plasma, but other congenital deficient plasmas, including those of individuals with Fletcher trait and Fitzgerald trait, contained normal amounts of HF antigen. HF antigen was reduced in the plasmas of patients with disseminated intravascular coagulation or advanced liver cirrhosis, but it was normal in those of patients with chronic renal failure or patients under treatment with warfarin. HF antigen was detected by this assay in plasmas of primates, but not detectable in plasmas of 11 nonprimate mammalian and one avian species.", "contents": "Radioimmunoassay of human Hageman factor (factor XII). A secific, sensitive, and reproducible radioimmunoassay for human Hageman factor (HF, factory XII) has been developed with purified human HF and monospecific rabbit antibody. Precise measurements of HF antigen were possible for concentrations as low as 0.1% of that in normal pooled plasma. A good correlation (correlation co-efficient = 0.82) existed between the titers of HF measured by clot-promoting assays and radioimmunoassays among 42 normal adults. Confirming earlier studies, HF antigen was absent in Hageman trait plasma, but other congenital deficient plasmas, including those of individuals with Fletcher trait and Fitzgerald trait, contained normal amounts of HF antigen. HF antigen was reduced in the plasmas of patients with disseminated intravascular coagulation or advanced liver cirrhosis, but it was normal in those of patients with chronic renal failure or patients under treatment with warfarin. HF antigen was detected by this assay in plasmas of primates, but not detectable in plasmas of 11 nonprimate mammalian and one avian species.", "PMID": 956701}
{"id": "pubmed23n0032_2341", "title": "Estimation of galactose-1-phosphate in blood spotted on filter paper.", "content": "A method is described for the enzymic estimation of galactose-1-phosphate (Gal-1-P) in blood which has been applied to filter paper and allowed to dry. The successful clinical management of patients with galactosemia depends upon exclusion of galactose from their diet. Earlier studies demonstrated that red cell Gal-1-P is a sensitive indicator of exposure of such patients to galactose. These earlier methods, however, required venipuncture, preparation of washed, packed red cells, and shipment of the sample in dry ice to a central laboratory. With the present method, capillary blood can be drawn by a nonphysician, applied to filter paper and mailed in a conventional envelope at ambient temperature. From this sample, the Gal-1-P content of the red cells can be determined, if the hematocrit is known. These conveniences should allow estimates of Gal-1-P at a frequency more appropirate for optimal dietary control.", "contents": "Estimation of galactose-1-phosphate in blood spotted on filter paper. A method is described for the enzymic estimation of galactose-1-phosphate (Gal-1-P) in blood which has been applied to filter paper and allowed to dry. The successful clinical management of patients with galactosemia depends upon exclusion of galactose from their diet. Earlier studies demonstrated that red cell Gal-1-P is a sensitive indicator of exposure of such patients to galactose. These earlier methods, however, required venipuncture, preparation of washed, packed red cells, and shipment of the sample in dry ice to a central laboratory. With the present method, capillary blood can be drawn by a nonphysician, applied to filter paper and mailed in a conventional envelope at ambient temperature. From this sample, the Gal-1-P content of the red cells can be determined, if the hematocrit is known. These conveniences should allow estimates of Gal-1-P at a frequency more appropirate for optimal dietary control.", "PMID": 956702}
{"id": "pubmed23n0032_2342", "title": "A simple, rapid, efficient method for the preparation of gamma 32P-labeled guanosine triphosphate (GTP) and adenosine triphosphate (ATP).", "content": "A simplified new method for synthesis fo gamma 32P GTP and gamma 32P ATP is described. Radiophosphorus is incorporated into GDP and ADP to form GTP and ATP, respectively, in the phosphoglycerate kinase reaction. The product is separated by ion exchange resin chromatography. The procedure is simple, gives a product a high purity, and the label is confined to the gamma position.", "contents": "A simple, rapid, efficient method for the preparation of gamma 32P-labeled guanosine triphosphate (GTP) and adenosine triphosphate (ATP). A simplified new method for synthesis fo gamma 32P GTP and gamma 32P ATP is described. Radiophosphorus is incorporated into GDP and ADP to form GTP and ATP, respectively, in the phosphoglycerate kinase reaction. The product is separated by ion exchange resin chromatography. The procedure is simple, gives a product a high purity, and the label is confined to the gamma position.", "PMID": 956703}
{"id": "pubmed23n0032_2343", "title": "A multiple-electrode array for a cochlear implant.", "content": "A satisfactory multiple-electrode array for a cochlear implant has been developed. This can be passed around the turns of the cochlea if it is first introduced into the scala tympani through an opening in the apical turn.", "contents": "A multiple-electrode array for a cochlear implant. A satisfactory multiple-electrode array for a cochlear implant has been developed. This can be passed around the turns of the cochlea if it is first introduced into the scala tympani through an opening in the apical turn.", "PMID": 956705}
{"id": "pubmed23n0032_2344", "title": "X-ray video-tape studies of laryngectomized patients.", "content": "The voices of forty-one laryngectomized patients were studied by X-ray video-tape. Three-quarters had good and one-quarter poor voices. A high-seated and short pseudoglottis affords the best results. The causes of the poor voices are discussed.", "contents": "X-ray video-tape studies of laryngectomized patients. The voices of forty-one laryngectomized patients were studied by X-ray video-tape. Three-quarters had good and one-quarter poor voices. A high-seated and short pseudoglottis affords the best results. The causes of the poor voices are discussed.", "PMID": 956706}
{"id": "pubmed23n0032_2345", "title": "Hereditary progressive sensorineural deafness.", "content": "Progressive sensorineural hearing losses found in seven members of three families are presented. Genetic transmission patterns in the study appeared to be autosomal dominant in two families and recessive in one family. The common audiological features of these cases include bilaterally symmetrical audiometric configuration and fairly good speech discrimination. The hearing losses of most of the cases appeared to begin at high frequencies progressing later to involve lower frequencies. The main histopathological changes in the temporal bones of one of the cases were degeneration of the organ of Corti and of the spiral ganglion in the lower cochlear coils and cystic degeneration of the stria vascularis in the upper coils.", "contents": "Hereditary progressive sensorineural deafness. Progressive sensorineural hearing losses found in seven members of three families are presented. Genetic transmission patterns in the study appeared to be autosomal dominant in two families and recessive in one family. The common audiological features of these cases include bilaterally symmetrical audiometric configuration and fairly good speech discrimination. The hearing losses of most of the cases appeared to begin at high frequencies progressing later to involve lower frequencies. The main histopathological changes in the temporal bones of one of the cases were degeneration of the organ of Corti and of the spiral ganglion in the lower cochlear coils and cystic degeneration of the stria vascularis in the upper coils.", "PMID": 956707}
{"id": "pubmed23n0032_2346", "title": "Pleomorphic  rhabdomyosarcoma of the larynx. A case report and review of the literature.", "content": "A case of pleomorphic rhabdomyosarcoma of the larynx occurring in a 33-year-old male is reported. The clinical and histopathological aspects of this undoubtedly rare neoplasm are described. After a quick review of the most important classifications of rhabdomyosarcoma so far made, the authors suggest a division into two basic groups, i.e. pleomorphic and fetal rhabdomyosarcoma, while the botryoid sarcoma and the alveolar rhabdomyosarcoma should be considered only as two subgroups of fetal rhabdomyosarcoma. From a comprehensive review of world literature on the subject, the well-documented cases up to now described are only seven, including the authors. The present case is the third one after the instances of pleomorphic rhabdomyosarcoma reported by Filipo and Crifo (1964 and by Rodriquez and Ziskind (1970).", "contents": "Pleomorphic  rhabdomyosarcoma of the larynx. A case report and review of the literature. A case of pleomorphic rhabdomyosarcoma of the larynx occurring in a 33-year-old male is reported. The clinical and histopathological aspects of this undoubtedly rare neoplasm are described. After a quick review of the most important classifications of rhabdomyosarcoma so far made, the authors suggest a division into two basic groups, i.e. pleomorphic and fetal rhabdomyosarcoma, while the botryoid sarcoma and the alveolar rhabdomyosarcoma should be considered only as two subgroups of fetal rhabdomyosarcoma. From a comprehensive review of world literature on the subject, the well-documented cases up to now described are only seven, including the authors. The present case is the third one after the instances of pleomorphic rhabdomyosarcoma reported by Filipo and Crifo (1964 and by Rodriquez and Ziskind (1970).", "PMID": 956708}
{"id": "pubmed23n0032_2347", "title": "Gelfilm and blowout fractures.", "content": "A variety of alloplastic and natural substances have been utilized for support of the orbital floor following a blowout fracture. The employment of Gelfilm is herein recommended for the smaller floor defects. Sterile Gelfilm is manufactured from denaturated collagen, is 0.075 mm. thick, and is known to be tolerated well by ocular tissues. Complete absorbsion of Gelfilm occurs in two to three months, obviating the need for removal of a prosthetic material or the lifetime presence of an alloplast in the orbit. In contrast to autografts no additional surgery is required for its preocurement.", "contents": "Gelfilm and blowout fractures. A variety of alloplastic and natural substances have been utilized for support of the orbital floor following a blowout fracture. The employment of Gelfilm is herein recommended for the smaller floor defects. Sterile Gelfilm is manufactured from denaturated collagen, is 0.075 mm. thick, and is known to be tolerated well by ocular tissues. Complete absorbsion of Gelfilm occurs in two to three months, obviating the need for removal of a prosthetic material or the lifetime presence of an alloplast in the orbit. In contrast to autografts no additional surgery is required for its preocurement.", "PMID": 956709}
{"id": "pubmed23n0032_2348", "title": "The natural history of chronic otitis media.", "content": "A retrospective study was made of 200 chronic otitis media patients. Simple chronic otitis media was observed in 76 per cent of cases; the rest were associated with cholesteatoma. In about one third of the patients, the contralateral ear showed some inflammatory middle ear disease as well. The average time lapse between initial symptoms and hospitalization was about 10 years. The events leading to the tympanic perforation were difficult to ascertain, but included probably acute otitis media, possibly external otitis, trauma, and a rather large group (35-40 per cent) of insidious 'essential perforations'. The aetiology of the 'essential perforations' is so far not known, but might be non-inflammatory in nature but related to insufficient middle ear aeration and hypo-pneumatization as well as to what is termed atelectatic ears. The bacteria isolated from chronic otitis media ears (usually gram negative bacteria and staphylococcus aureus) are usually not the types of micro-organisms found in association with any primary or acute otitis media. It is proposed that the bacterial infection encountered in what is termed 'chronic otitis media' is often a secondary infection of a primary perforated tympanic membrane, the perforation originating or persisting in underventilated ears, and having arisen from various causes--some of them as yet unknown.", "contents": "The natural history of chronic otitis media. A retrospective study was made of 200 chronic otitis media patients. Simple chronic otitis media was observed in 76 per cent of cases; the rest were associated with cholesteatoma. In about one third of the patients, the contralateral ear showed some inflammatory middle ear disease as well. The average time lapse between initial symptoms and hospitalization was about 10 years. The events leading to the tympanic perforation were difficult to ascertain, but included probably acute otitis media, possibly external otitis, trauma, and a rather large group (35-40 per cent) of insidious 'essential perforations'. The aetiology of the 'essential perforations' is so far not known, but might be non-inflammatory in nature but related to insufficient middle ear aeration and hypo-pneumatization as well as to what is termed atelectatic ears. The bacteria isolated from chronic otitis media ears (usually gram negative bacteria and staphylococcus aureus) are usually not the types of micro-organisms found in association with any primary or acute otitis media. It is proposed that the bacterial infection encountered in what is termed 'chronic otitis media' is often a secondary infection of a primary perforated tympanic membrane, the perforation originating or persisting in underventilated ears, and having arisen from various causes--some of them as yet unknown.", "PMID": 956712}
{"id": "pubmed23n0032_2349", "title": "Dacryocystantrostomy. A preliminary report.", "content": "A new operative technique is described in which the lacrimal sac is drained into the maxillary sinus. The advantages of the procedure, especially of avoiding a facial incision, are emphasized. Five patients suffering from long-standing epiphora underwent surgery. Two patients had chronic dacryocystitis. Two other patients presented with a mucocele of the lacrimal sac. In the fifth patient, there was obstruction in the nasolacrimal duct. Short term follow-up results suggest its usefulness as a first line of surgical treatment in cases of epiphora due to obstruction in the lacrimal sac or the naso-lacrimal duct.", "contents": "Dacryocystantrostomy. A preliminary report. A new operative technique is described in which the lacrimal sac is drained into the maxillary sinus. The advantages of the procedure, especially of avoiding a facial incision, are emphasized. Five patients suffering from long-standing epiphora underwent surgery. Two patients had chronic dacryocystitis. Two other patients presented with a mucocele of the lacrimal sac. In the fifth patient, there was obstruction in the nasolacrimal duct. Short term follow-up results suggest its usefulness as a first line of surgical treatment in cases of epiphora due to obstruction in the lacrimal sac or the naso-lacrimal duct.", "PMID": 956713}
{"id": "pubmed23n0032_2350", "title": "Improvement of recalcitrant psoriasis vulgaris after tonsillectomy.", "content": "To study the effect of tonsillectomy as a possible part of the treatment of infection-released, relapsing and recalcitrant (to topical therapy and courses of penicillin) psoriasis vulgaris in children and adolescents and to test a possible correlation between tonsillitis and exacerbations of psoriasis vulgaris, a retrospective study (charts and questionnaires) of the course of psoriasis after tonsillectomy was undertaken in 74 patients with such psoriasis. Each patient served as his own control. At tonsillectomy the average age of patients and the duration of psoriasis were 14-2 years and 4-5 years, respectively, while the average follow-up period was 4-5 years. The clearing of psoriasis vulgaris was stastically significant, p less than 0-01, as 1/3 of the patients obtained clearing of psoriasis throughout the entire follow-up period, while an additional 1/3 noticed considerable improvement of their psoriasis. After having tried both topical therapy of various sorts and courses of penicillin, tonsillectomy might be taken into consideration in relapsing, recalcitrant psoriasis vulgaris in children and adolescents.", "contents": "Improvement of recalcitrant psoriasis vulgaris after tonsillectomy. To study the effect of tonsillectomy as a possible part of the treatment of infection-released, relapsing and recalcitrant (to topical therapy and courses of penicillin) psoriasis vulgaris in children and adolescents and to test a possible correlation between tonsillitis and exacerbations of psoriasis vulgaris, a retrospective study (charts and questionnaires) of the course of psoriasis after tonsillectomy was undertaken in 74 patients with such psoriasis. Each patient served as his own control. At tonsillectomy the average age of patients and the duration of psoriasis were 14-2 years and 4-5 years, respectively, while the average follow-up period was 4-5 years. The clearing of psoriasis vulgaris was stastically significant, p less than 0-01, as 1/3 of the patients obtained clearing of psoriasis throughout the entire follow-up period, while an additional 1/3 noticed considerable improvement of their psoriasis. After having tried both topical therapy of various sorts and courses of penicillin, tonsillectomy might be taken into consideration in relapsing, recalcitrant psoriasis vulgaris in children and adolescents.", "PMID": 956714}
{"id": "pubmed23n0032_2351", "title": "Positive 'fistula sign' with intact ear-drum in a patient with eosinophil granuloma of the mastoid.", "content": "A case is presented of a positive 'fistula sign' in a patient with an intact ear-drum and no history of previous ear-disease. The cause proved to be an eosinophil granuloma of the temporal bone which responded well to radiotherapy.", "contents": "Positive 'fistula sign' with intact ear-drum in a patient with eosinophil granuloma of the mastoid. A case is presented of a positive 'fistula sign' in a patient with an intact ear-drum and no history of previous ear-disease. The cause proved to be an eosinophil granuloma of the temporal bone which responded well to radiotherapy.", "PMID": 956716}
{"id": "pubmed23n0032_2352", "title": "Sensorineural deafness due to compression chamber noise.", "content": "A case of unilateral sensorineural deafness following exposure to compression chamber noise is described. A review of the current literature concerning the otological hazards of compression chambers is made. The possible pathological basis is discussed.", "contents": "Sensorineural deafness due to compression chamber noise. A case of unilateral sensorineural deafness following exposure to compression chamber noise is described. A review of the current literature concerning the otological hazards of compression chambers is made. The possible pathological basis is discussed.", "PMID": 956717}
{"id": "pubmed23n0032_2353", "title": "Cervicoauricular fistula.", "content": "(I) A rare case of cervicoauricular fistula with a review of available literature has been presented. (2) The cephalic end of a fistulous tract showing elevations and depressions probably represents the duplication of tubercles taking part in the formation of external ear.", "contents": "Cervicoauricular fistula. (I) A rare case of cervicoauricular fistula with a review of available literature has been presented. (2) The cephalic end of a fistulous tract showing elevations and depressions probably represents the duplication of tubercles taking part in the formation of external ear.", "PMID": 956718}
{"id": "pubmed23n0032_2354", "title": "Regulation by the H-2 gene complex of macrophage-lymphoid cell interactions in secondary antibody responses in vitro.", "content": "The ability of antigen-bearing syngeneic and allogeneic peptone-induced peritoneal exudate macrophages to support development of primary and secondary antibody responses by murine lymphoid or spleen cells in vitro has been investigated. The antigen used was the terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). Syngeneic and allogeneic macrophages supported development of comparable primary antibody responses to GAT, indicating that genetic restrictions do not limit efficient macrophage-lymphocyte interactions in primary responses. By contrast, immunized spleen or lymphoid cells developed secondary antibody responses preferentially when stimulated in vitro with GAT on macrophages syngeneic to the macrophages used to present GAT during in vivo immunization. Thus, genetic restrictions regulate efficient macrophage-lymphocyte interactions in secondary antibody responses. These restrictions have been demonstrated from 2 to 8 wk after a single immunization with limiting quantities of GAT and are controlled by the H-2 gene complex. The implications that immune lymphocytes selectively recognize and respond to antigen presented in the context of the macrophage membrane-antigen complex which sensitized the lymphocytes initially are considered.", "contents": "Regulation by the H-2 gene complex of macrophage-lymphoid cell interactions in secondary antibody responses in vitro. The ability of antigen-bearing syngeneic and allogeneic peptone-induced peritoneal exudate macrophages to support development of primary and secondary antibody responses by murine lymphoid or spleen cells in vitro has been investigated. The antigen used was the terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). Syngeneic and allogeneic macrophages supported development of comparable primary antibody responses to GAT, indicating that genetic restrictions do not limit efficient macrophage-lymphocyte interactions in primary responses. By contrast, immunized spleen or lymphoid cells developed secondary antibody responses preferentially when stimulated in vitro with GAT on macrophages syngeneic to the macrophages used to present GAT during in vivo immunization. Thus, genetic restrictions regulate efficient macrophage-lymphocyte interactions in secondary antibody responses. These restrictions have been demonstrated from 2 to 8 wk after a single immunization with limiting quantities of GAT and are controlled by the H-2 gene complex. The implications that immune lymphocytes selectively recognize and respond to antigen presented in the context of the macrophage membrane-antigen complex which sensitized the lymphocytes initially are considered.", "PMID": 956724}
{"id": "pubmed23n0032_2355", "title": "Association of serum hemolytic complement levels with the major histocompatibility complex in chickens.", "content": "The total hemolytic complement (C) levels in inbred line 7 chicks and adults were lower than C levels in inbred lines 2 and 3 and in outbred chickens of the same age. In all birds, adult levels of C were obtained in 5- to 6-wk-old chickens. Analysis of F1 and F3 generations clearly showed that the C level in chickens was determined by a dominant gene(s) associated with the major histocompatibility complex. Finding this association in a nonmammal strengthens the importance of the relationship between closely linked genes controlling histocompatibility, immune responsiveness, mixed leukocyte reaction, and C activity.", "contents": "Association of serum hemolytic complement levels with the major histocompatibility complex in chickens. The total hemolytic complement (C) levels in inbred line 7 chicks and adults were lower than C levels in inbred lines 2 and 3 and in outbred chickens of the same age. In all birds, adult levels of C were obtained in 5- to 6-wk-old chickens. Analysis of F1 and F3 generations clearly showed that the C level in chickens was determined by a dominant gene(s) associated with the major histocompatibility complex. Finding this association in a nonmammal strengthens the importance of the relationship between closely linked genes controlling histocompatibility, immune responsiveness, mixed leukocyte reaction, and C activity.", "PMID": 956725}
{"id": "pubmed23n0032_2356", "title": "The synthesis and secretion of immunoglobulins by lymphoid cells in the sheep. The primary response to salmonella lipopolysaccharide.", "content": "The primary response of the popliteal node to Salmonella lipopolysaccharide was studied in the sheep. All three classes of immunoglobulin IgG1, igG2, and IgM were produced by both free-floating cells in the lymph and by cells within the pymph node throughout the immune responce which extended over a period of at least 20 days.. Most of the immunoglobulins were found to be nonspecific for the antigen when tested by a binding assay. It was calculated from the binding assay that far more antigen-specific IgG molecules were produced than IgM molecules. The proportion of IgM and IgG1 which showed affinity for Salmonella organisms increased throughout the response. IgG2 had no affinity for the antigen until around 480 h after challenge. When a hemagglutination assay was used to measure antibody production, most of the specific antibody produced during the response was found to be IgM. Blast cells produced most of the immunoglobulin during the first 4 days of the response, and these cells were responsible for almost all of the IgM production. Differences were observed in the relative amounts of IgG and IgM produced by the cells within the node and by free-floating cells in the efferent lymph. The free-floating cells in lymph synthesized and secreted relatively more IgM and relatively less IgG than did cells within the lymph node. Both populations of cells, however, secreted much more IgG than IgM.", "contents": "The synthesis and secretion of immunoglobulins by lymphoid cells in the sheep. The primary response to salmonella lipopolysaccharide. The primary response of the popliteal node to Salmonella lipopolysaccharide was studied in the sheep. All three classes of immunoglobulin IgG1, igG2, and IgM were produced by both free-floating cells in the lymph and by cells within the pymph node throughout the immune responce which extended over a period of at least 20 days.. Most of the immunoglobulins were found to be nonspecific for the antigen when tested by a binding assay. It was calculated from the binding assay that far more antigen-specific IgG molecules were produced than IgM molecules. The proportion of IgM and IgG1 which showed affinity for Salmonella organisms increased throughout the response. IgG2 had no affinity for the antigen until around 480 h after challenge. When a hemagglutination assay was used to measure antibody production, most of the specific antibody produced during the response was found to be IgM. Blast cells produced most of the immunoglobulin during the first 4 days of the response, and these cells were responsible for almost all of the IgM production. Differences were observed in the relative amounts of IgG and IgM produced by the cells within the node and by free-floating cells in the efferent lymph. The free-floating cells in lymph synthesized and secreted relatively more IgM and relatively less IgG than did cells within the lymph node. Both populations of cells, however, secreted much more IgG than IgM.", "PMID": 956726}
{"id": "pubmed23n0032_2357", "title": "Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules.", "content": "An in vitro model is described for studying the interaction between lymphocytes and high-endothelial venules (HEV) of lymph nodes. Rat or mouse lymphocytes which were layered over fixed sections of syngeneic lymph nodes adhered selectively to the endothelium of HEV but did not bind to other vascular endothelia. Evidence is presented that adherence to HEV in vitro is a property of recirculating lymphocytes and not a characteristic of cells which are unable to home into lymph nodes in vivo.", "contents": "Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. An in vitro model is described for studying the interaction between lymphocytes and high-endothelial venules (HEV) of lymph nodes. Rat or mouse lymphocytes which were layered over fixed sections of syngeneic lymph nodes adhered selectively to the endothelium of HEV but did not bind to other vascular endothelia. Evidence is presented that adherence to HEV in vitro is a property of recirculating lymphocytes and not a characteristic of cells which are unable to home into lymph nodes in vivo.", "PMID": 956727}
{"id": "pubmed23n0032_2358", "title": "Total plasma porphyrins in renal disease.", "content": "Thirty patients suffering from chronic kidney disease were examined for urinary, fecal and total plasma porphyrins. Twenty patients had nitrogen retention whereas the remaining ten patients displayed normal values. Urinary coproporphyrin excretion and total plasma porphyrin levels were comparatively less pronounced in patients with nitrogen retention. Such changes in porphyrin metabolism could be explained by a diminished capacity for kidney excretion. It is concluded that the total amount of porphyrins synthesized in the body is decreased as a result of nitrogen retention.", "contents": "Total plasma porphyrins in renal disease. Thirty patients suffering from chronic kidney disease were examined for urinary, fecal and total plasma porphyrins. Twenty patients had nitrogen retention whereas the remaining ten patients displayed normal values. Urinary coproporphyrin excretion and total plasma porphyrin levels were comparatively less pronounced in patients with nitrogen retention. Such changes in porphyrin metabolism could be explained by a diminished capacity for kidney excretion. It is concluded that the total amount of porphyrins synthesized in the body is decreased as a result of nitrogen retention.", "PMID": 956728}
{"id": "pubmed23n0032_2359", "title": "Evaluation of the Abbott Bichromatic Analyzer 100 (A proposal for an evaluation scheme).", "content": "This report summarizes a one year evaluation of Abbott's ABA 100, with respect to mechanical parts (syringe plates, precision and linearity of photometry, band width of several filters, multicuvet precision, temperature control) and the reliability of several methods (endpoint procedures: determination of the glucose concentration with hexokinase- and the glucose dehydrogenase method, and of the protein concentration; enzyme activities: alanine and aspartate aminotransferase, creatine kinase, alkaline phosphatase). The critical batch size was estimated as an indicator of economy (about 40 samples per day for the glucose concentration). Various aspects of the instrument are discussed with respect to its use in clinical chemistry.", "contents": "Evaluation of the Abbott Bichromatic Analyzer 100 (A proposal for an evaluation scheme). This report summarizes a one year evaluation of Abbott's ABA 100, with respect to mechanical parts (syringe plates, precision and linearity of photometry, band width of several filters, multicuvet precision, temperature control) and the reliability of several methods (endpoint procedures: determination of the glucose concentration with hexokinase- and the glucose dehydrogenase method, and of the protein concentration; enzyme activities: alanine and aspartate aminotransferase, creatine kinase, alkaline phosphatase). The critical batch size was estimated as an indicator of economy (about 40 samples per day for the glucose concentration). Various aspects of the instrument are discussed with respect to its use in clinical chemistry.", "PMID": 956729}
{"id": "pubmed23n0032_2360", "title": "[Further characterisation of trypsin inhibitors in the polychaet Sabellastarte indica (Savigny), II (author's transl)].", "content": "In this paper we discribe a method for the isolation of trypsin inhibitors from the tentacles of the annelid Sabellastarte indica Savigny. These inhibitors - now homogeneous in their molecular weight - can be characterised by sodium dodecylsulfate-acrylamide gel-electrophoresis, in their inhibitory activity towards trypsin, plasmin and chymotrypsin. The inhibitors from Sabellastarte indica possess a stoichiometric binding relation of 2:1 for trypsin, lysine being the amino-acid in the reactive centre of the inhibitor responsible for interaction with trypsin. The reactive centre for trypsin is not identical with the reactive centre which binds chymotrypsin and does not influence the binding of chymotrypsin. These newly described inhibitors are therefore multiheaded, a type not previously described for invertebrates.", "contents": "[Further characterisation of trypsin inhibitors in the polychaet Sabellastarte indica (Savigny), II (author's transl)]. In this paper we discribe a method for the isolation of trypsin inhibitors from the tentacles of the annelid Sabellastarte indica Savigny. These inhibitors - now homogeneous in their molecular weight - can be characterised by sodium dodecylsulfate-acrylamide gel-electrophoresis, in their inhibitory activity towards trypsin, plasmin and chymotrypsin. The inhibitors from Sabellastarte indica possess a stoichiometric binding relation of 2:1 for trypsin, lysine being the amino-acid in the reactive centre of the inhibitor responsible for interaction with trypsin. The reactive centre for trypsin is not identical with the reactive centre which binds chymotrypsin and does not influence the binding of chymotrypsin. These newly described inhibitors are therefore multiheaded, a type not previously described for invertebrates.", "PMID": 956730}
{"id": "pubmed23n0032_2361", "title": "[Calculation of radioimmunochemical determinations by \"spline approximation\" (author's transl)].", "content": "A simplified method, based on the \"spline approximation\", is reported for the calculation of the standard curves of radioimmunochemical determinations. It is possible to manipulate the mathematical function with a pocket calculator, thus making it available for a large number of users. It was shown that, in contrast to the usual procedures, it is possible to achieve optimal quality control in the preparation of the standard curves and in the interpolation of unknown plasma samples. The recaluculation of interpolated values from their own standard curve revealed an error of 4.9% which would normally be an error of interpolation. The new method was compared with two established methods for 8 different radioimmunochemical determinations. The measured values of the standard curve showed a weighting, and there was a resulting quality control of these values, which, according to their statistical evalution, were more accurate than those of the others models (Ekins et al., Yalow et al., (1968), in: Radioisotopes in Medicine: in vitro studies (Hayes, R. L., Goswitz, F.A. & Murphy, B. E. P., eds) USA EC, Oak Ridge) and Rodbard et al. (1971), in: Competitive protein Binding Assys(Odell, W. D. & Danghedy, W. H., eds.) Lipincott, Philadelphia and Toronto). In contrast with these other models, the described method makes no mathematical or kinetic preconditions with respect to the dose-response relationship. To achieve optimal reaction conditions, experimentally determined reaction data are preferable to model theories.", "contents": "[Calculation of radioimmunochemical determinations by \"spline approximation\" (author's transl)]. A simplified method, based on the \"spline approximation\", is reported for the calculation of the standard curves of radioimmunochemical determinations. It is possible to manipulate the mathematical function with a pocket calculator, thus making it available for a large number of users. It was shown that, in contrast to the usual procedures, it is possible to achieve optimal quality control in the preparation of the standard curves and in the interpolation of unknown plasma samples. The recaluculation of interpolated values from their own standard curve revealed an error of 4.9% which would normally be an error of interpolation. The new method was compared with two established methods for 8 different radioimmunochemical determinations. The measured values of the standard curve showed a weighting, and there was a resulting quality control of these values, which, according to their statistical evalution, were more accurate than those of the others models (Ekins et al., Yalow et al., (1968), in: Radioisotopes in Medicine: in vitro studies (Hayes, R. L., Goswitz, F.A. & Murphy, B. E. P., eds) USA EC, Oak Ridge) and Rodbard et al. (1971), in: Competitive protein Binding Assys(Odell, W. D. & Danghedy, W. H., eds.) Lipincott, Philadelphia and Toronto). In contrast with these other models, the described method makes no mathematical or kinetic preconditions with respect to the dose-response relationship. To achieve optimal reaction conditions, experimentally determined reaction data are preferable to model theories.", "PMID": 956731}
{"id": "pubmed23n0032_2362", "title": "[Kinetic determination of urea with the LKB system (author's transl)].", "content": "The kinetic determination of urea based on the urea/glutamate dehydrogenase method was adapted for the LKB Reaction Rate Analyzer System 8600. A ratio of sample to reagent volume of 1:50 ensures linearity up to 33.3 mmol/l with a day to day precision of 5%. Parallel studies with the urease/glutamate dehydrogenase method were performed with the CentrifiChem System and with the manual Berthelot/Salicylate method.", "contents": "[Kinetic determination of urea with the LKB system (author's transl)]. The kinetic determination of urea based on the urea/glutamate dehydrogenase method was adapted for the LKB Reaction Rate Analyzer System 8600. A ratio of sample to reagent volume of 1:50 ensures linearity up to 33.3 mmol/l with a day to day precision of 5%. Parallel studies with the urease/glutamate dehydrogenase method were performed with the CentrifiChem System and with the manual Berthelot/Salicylate method.", "PMID": 956732}
{"id": "pubmed23n0032_2363", "title": "Cooperative study on the radioimmunological thyrotropin determination (hTSH) in serum (author's transl).", "content": "This paper deals with a cooperative survey on the radioimmunoassay of thyrotropin in human serum, which was organized by the Thyroid Group of the German Society for Endocrinology. Eighteen serum specimens were to be measured in triplicates. Among the latter were 10 specimens of a hTSH standard curve, prepared in low hTSH serum, which contained approximately 1 mU/l. One of these pool sera was divided in half, and one half was enriched with 10 kU/l human chorionic gonadotropin to test for cross reactivity. One specimen las for the measurement of the non-specific binding. The count rates and the dose levels of the standard curve of each participating laboratory (N = 32), normally performed in albumin-buffer, and the count rates of the 18 unknowns were requested as results. THE CALCULATION OF THE STANDARD CURVES AND THE RECOVERy curves, the evaluation of the unknowns and the statistics were performed by spline functions, using a 48 kb FORTRAN-program on a Siemens 404/3 computer. The results demonstrated that the interlaboratory precision of 128% and the recovery rates between 50 and 926% are very unsatisfactory. However, the coefficient of variation was lowered to 31%, when the controls were read from the recovery curves, as included in the samples. In addition to this improvement of the interlaboratory precision and of the accuracy, this study showed, that the sensitivity was markedly improved by using delayed addition of tracer.", "contents": "Cooperative study on the radioimmunological thyrotropin determination (hTSH) in serum (author's transl). This paper deals with a cooperative survey on the radioimmunoassay of thyrotropin in human serum, which was organized by the Thyroid Group of the German Society for Endocrinology. Eighteen serum specimens were to be measured in triplicates. Among the latter were 10 specimens of a hTSH standard curve, prepared in low hTSH serum, which contained approximately 1 mU/l. One of these pool sera was divided in half, and one half was enriched with 10 kU/l human chorionic gonadotropin to test for cross reactivity. One specimen las for the measurement of the non-specific binding. The count rates and the dose levels of the standard curve of each participating laboratory (N = 32), normally performed in albumin-buffer, and the count rates of the 18 unknowns were requested as results. THE CALCULATION OF THE STANDARD CURVES AND THE RECOVERy curves, the evaluation of the unknowns and the statistics were performed by spline functions, using a 48 kb FORTRAN-program on a Siemens 404/3 computer. The results demonstrated that the interlaboratory precision of 128% and the recovery rates between 50 and 926% are very unsatisfactory. However, the coefficient of variation was lowered to 31%, when the controls were read from the recovery curves, as included in the samples. In addition to this improvement of the interlaboratory precision and of the accuracy, this study showed, that the sensitivity was markedly improved by using delayed addition of tracer.", "PMID": 956733}
{"id": "pubmed23n0032_2364", "title": "Automated amino acid analysis with sensitive fluorescence detection.", "content": "The fluorigenic o-phthalaldehyde-mercaptoethanol reagent gives good reproducibility with a very stable baseline when applied to the automated analysis of amino acids at the nanomole level. Determination of even smaller quantities is possible; basic amino acids are then preferably eluted separately at constant pH (for example pH 6.0); this eliminates the baseline irregularities that occur with single column systems at high sensitivity settings. The reagent gives excellent results in the assay of small quantities of biological fluids such as blood plasma.", "contents": "Automated amino acid analysis with sensitive fluorescence detection. The fluorigenic o-phthalaldehyde-mercaptoethanol reagent gives good reproducibility with a very stable baseline when applied to the automated analysis of amino acids at the nanomole level. Determination of even smaller quantities is possible; basic amino acids are then preferably eluted separately at constant pH (for example pH 6.0); this eliminates the baseline irregularities that occur with single column systems at high sensitivity settings. The reagent gives excellent results in the assay of small quantities of biological fluids such as blood plasma.", "PMID": 956734}
{"id": "pubmed23n0032_2365", "title": "[Action of the \"Ca-ionophore\" X-537A on the electrolyte content and metabolism of Yoshida ascites tumour cells: a model for the analysis of Mg deficiency (author's transl)].", "content": "X-537A, an ionophore for divalent cations, causes a rapid replacement of extracellular Na+ by intracellular K+ in Yoshida ascites tumour cells. The loss of K+ is prevented by high extracellular concentrations of K+. In incubation solutions containing Ca2+, the Ca2+ content of the tumour cells increases in the presence of X-537A, but the Mg2+ content is not affected. The intracellular level of cyclic AMP is also increased. In all the incubation media used, the presence of X-537A resulted in a decrease in biosynthesis in the order RNA greater than DNA greater than protein, at constant ATP concentration. The observed changes in the content of electrolytes and cyclic AMP, and on the metabolism of DNA, RNA and protein in Yoshida ascites tumour cells in response to X-537A, correspond to those observed during Mg deficiency in these cells. These changes are caused by changes in the intracellular concentrations of Ca2+ and/or K+.", "contents": "[Action of the \"Ca-ionophore\" X-537A on the electrolyte content and metabolism of Yoshida ascites tumour cells: a model for the analysis of Mg deficiency (author's transl)]. X-537A, an ionophore for divalent cations, causes a rapid replacement of extracellular Na+ by intracellular K+ in Yoshida ascites tumour cells. The loss of K+ is prevented by high extracellular concentrations of K+. In incubation solutions containing Ca2+, the Ca2+ content of the tumour cells increases in the presence of X-537A, but the Mg2+ content is not affected. The intracellular level of cyclic AMP is also increased. In all the incubation media used, the presence of X-537A resulted in a decrease in biosynthesis in the order RNA greater than DNA greater than protein, at constant ATP concentration. The observed changes in the content of electrolytes and cyclic AMP, and on the metabolism of DNA, RNA and protein in Yoshida ascites tumour cells in response to X-537A, correspond to those observed during Mg deficiency in these cells. These changes are caused by changes in the intracellular concentrations of Ca2+ and/or K+.", "PMID": 956735}
{"id": "pubmed23n0032_2366", "title": "[Determination of glucose with glucoseoxidase-UV, using hexokinase as the reference method (author's transl)].", "content": "Results obtained with the commercial test-set \"GOD-UV\" (Dr. Haury, M\u00fcnchen) are reported. This method permits the determination of serum-glucose within 3 minutes. The procedure is satisfactory in normal and hyperglycaemic patients. In the hypoglycaemic state, however, there is a danger of misinterpretation. The glucose oxidase-UV is compared with the hexokinase reaction.", "contents": "[Determination of glucose with glucoseoxidase-UV, using hexokinase as the reference method (author's transl)]. Results obtained with the commercial test-set \"GOD-UV\" (Dr. Haury, M\u00fcnchen) are reported. This method permits the determination of serum-glucose within 3 minutes. The procedure is satisfactory in normal and hyperglycaemic patients. In the hypoglycaemic state, however, there is a danger of misinterpretation. The glucose oxidase-UV is compared with the hexokinase reaction.", "PMID": 956736}
{"id": "pubmed23n0032_2367", "title": "[Gaschromatographic method for routine determination of pregnanediol and pregnanetriol in urine (author's transl)].", "content": "A rapid, highly sensitive and simple method for the routine measurement of urinary pregnanediol and pregnanetriol involving enzymatic hydrolysis, extraction with toluene and gas liquid chromatography is described.", "contents": "[Gaschromatographic method for routine determination of pregnanediol and pregnanetriol in urine (author's transl)]. A rapid, highly sensitive and simple method for the routine measurement of urinary pregnanediol and pregnanetriol involving enzymatic hydrolysis, extraction with toluene and gas liquid chromatography is described.", "PMID": 956737}
{"id": "pubmed23n0032_2368", "title": "Death from intravenously administered narcotics: a study of 114 cases;.", "content": "A careful study of more than 100 fatal cases due to intravenously administered narcotics provides further evidence of the complexity involved in the certification of death. The wide range of blood morphine concentrations found in these cases indicates that tissue concentrations alone will not always provide the necessary information. High concentrations of morphine, the major metabolite of heroin, in blood and other tissues may be consistent with overdose. But in those cases involving very low concentrations at the time of death, other criteria must be considered.", "contents": "Death from intravenously administered narcotics: a study of 114 cases;. A careful study of more than 100 fatal cases due to intravenously administered narcotics provides further evidence of the complexity involved in the certification of death. The wide range of blood morphine concentrations found in these cases indicates that tissue concentrations alone will not always provide the necessary information. High concentrations of morphine, the major metabolite of heroin, in blood and other tissues may be consistent with overdose. But in those cases involving very low concentrations at the time of death, other criteria must be considered.", "PMID": 956738}
{"id": "pubmed23n0032_2369", "title": "Accidental asphyxial deaths involving infants and young children.", "content": "Forty-five cases of accidental asphyxial deaths in infants and young children below 2 years of age are described. The mechanism involved, the available historical information, and the possible preventive measures are discussed. Dangers such as bed- and tub-sharing, diaper and cleaning pails, plastic wrappers, balloons, small beds, toys on strings, broken or poorly designed cribs, and poorly positioned adult beds must be brought to the attention of the parent as consumer. The public heatlh considerations and the educational aspects relating to the community at large are cornerstones in the effort to reduce these tragic and untimely deaths.", "contents": "Accidental asphyxial deaths involving infants and young children. Forty-five cases of accidental asphyxial deaths in infants and young children below 2 years of age are described. The mechanism involved, the available historical information, and the possible preventive measures are discussed. Dangers such as bed- and tub-sharing, diaper and cleaning pails, plastic wrappers, balloons, small beds, toys on strings, broken or poorly designed cribs, and poorly positioned adult beds must be brought to the attention of the parent as consumer. The public heatlh considerations and the educational aspects relating to the community at large are cornerstones in the effort to reduce these tragic and untimely deaths.", "PMID": 956739}
{"id": "pubmed23n0032_2370", "title": "Studies by crossed electroimmunodiffusion on the individuality and sexual orgin of bloodstains.", "content": "Crossed electroimmunodiffusion (CEID) was evaluated as a means for individualizing human blood stains by studying variations within and among individuals in 22 serum antigens in ten subjects over a four-month period. The extent of variation within an individual was determined by making CEID runs on blood stains obtained on ten different occasions and measuring the precipitin peak heights produced by each of the 22 antigens. When the range in height of any particular peak was completely different in subject-subject comparisons, the peak was judged to be of value in individualization. By this criterion, each of the ten subjects could be distinguished from all others (65 subject-subject distinctions), but in most cases the distinction had to be based on difference in 5 or less of the 22 antigens. The antigens of value in distinguishing among males were largely different from those of value in distinguishing among females. Overall, the antigens of greatest value in individualization were 8, 9, 10, and 11. Only one of these (10, ceruloplasmin) could be identified as a particular serum proteins.", "contents": "Studies by crossed electroimmunodiffusion on the individuality and sexual orgin of bloodstains. Crossed electroimmunodiffusion (CEID) was evaluated as a means for individualizing human blood stains by studying variations within and among individuals in 22 serum antigens in ten subjects over a four-month period. The extent of variation within an individual was determined by making CEID runs on blood stains obtained on ten different occasions and measuring the precipitin peak heights produced by each of the 22 antigens. When the range in height of any particular peak was completely different in subject-subject comparisons, the peak was judged to be of value in individualization. By this criterion, each of the ten subjects could be distinguished from all others (65 subject-subject distinctions), but in most cases the distinction had to be based on difference in 5 or less of the 22 antigens. The antigens of value in distinguishing among males were largely different from those of value in distinguishing among females. Overall, the antigens of greatest value in individualization were 8, 9, 10, and 11. Only one of these (10, ceruloplasmin) could be identified as a particular serum proteins.", "PMID": 956740}
{"id": "pubmed23n0032_2371", "title": "Probabilities and human pubic hair comparisons.", "content": "By use of a card coding system for some macroscopic and microscopic characteristics of human pubic hairs, 101 368 comparisons were made between 454 hairs from 60 different individuals. Of these, 16 pairs were found to be similar. For a Caucasian population, it is estimated that if one human pubic hair is found to be similar to at least one of a group of six to eleven mutually dissimilar hairs from a given source, the probability that it could have originated from another source is small, about 1 in 800.", "contents": "Probabilities and human pubic hair comparisons. By use of a card coding system for some macroscopic and microscopic characteristics of human pubic hairs, 101 368 comparisons were made between 454 hairs from 60 different individuals. Of these, 16 pairs were found to be similar. For a Caucasian population, it is estimated that if one human pubic hair is found to be similar to at least one of a group of six to eleven mutually dissimilar hairs from a given source, the probability that it could have originated from another source is small, about 1 in 800.", "PMID": 956741}
{"id": "pubmed23n0032_2372", "title": "Antemortem conversion of codeine to morphine in man.", "content": "Forty-five drug overdose cases involving codeine were investigated. Concentrations of codeine and morphine were determined in blood, bile, liver, kidney, and urine. Ratios of codeine to morphine values for each of these specimens were compared and evidence was developed that codeine was metabolized partially into morphine in the antemortem stage. Morphine concentration was less than that of codeine in blood, liver, kidney, and urine. However, bile analyses showed that the amount of morphine exceeded that of codeine, suggesting a more active demethylation activity in the hepatic system than in the blood and other tissues studied. Controlled-in-vitro studies showed that no codeine demethylation occurred in postmortem tissues during cold storage for a period as long as 30 days.", "contents": "Antemortem conversion of codeine to morphine in man. Forty-five drug overdose cases involving codeine were investigated. Concentrations of codeine and morphine were determined in blood, bile, liver, kidney, and urine. Ratios of codeine to morphine values for each of these specimens were compared and evidence was developed that codeine was metabolized partially into morphine in the antemortem stage. Morphine concentration was less than that of codeine in blood, liver, kidney, and urine. However, bile analyses showed that the amount of morphine exceeded that of codeine, suggesting a more active demethylation activity in the hepatic system than in the blood and other tissues studied. Controlled-in-vitro studies showed that no codeine demethylation occurred in postmortem tissues during cold storage for a period as long as 30 days.", "PMID": 956742}
{"id": "pubmed23n0032_2373", "title": "Identification of drugs and other toxic compounds from their ultraviolet spectra. Part III: Ultraviolet absorption properties of 22 structural groups.", "content": "The ultraviolet absorption spectra of 22 different chemical (structural) groups of drugs and toxic compounds were studied. This paper completes a three-part series in which more than 500 individual compounds have been grouped according to structure as it pertains to characteristics of the ultraviolet absorption scan. Each group has a typical absorption profile with respect to the number of bands between 200 and 340 nm, the intensity of the band(s), and the changes in absorption pattern with solvent and pH changes. Phenothiazines, xanthines, coumarins, quinolines, naphthalene derivatives. O-alkyl benzene derivatives, opiates, ergot alkaloids, benzodiazepines, and various heterocyclic compounds are among the groups of compounds covered in this paper.", "contents": "Identification of drugs and other toxic compounds from their ultraviolet spectra. Part III: Ultraviolet absorption properties of 22 structural groups. The ultraviolet absorption spectra of 22 different chemical (structural) groups of drugs and toxic compounds were studied. This paper completes a three-part series in which more than 500 individual compounds have been grouped according to structure as it pertains to characteristics of the ultraviolet absorption scan. Each group has a typical absorption profile with respect to the number of bands between 200 and 340 nm, the intensity of the band(s), and the changes in absorption pattern with solvent and pH changes. Phenothiazines, xanthines, coumarins, quinolines, naphthalene derivatives. O-alkyl benzene derivatives, opiates, ergot alkaloids, benzodiazepines, and various heterocyclic compounds are among the groups of compounds covered in this paper.", "PMID": 956743}
{"id": "pubmed23n0032_2374", "title": "Identification of drugs and their derivatives.", "content": "This paper represents a brief review of traditional procedures for the analysis of confiscated drugs and the application of these procedures to the products formed after reductive fragmentation and halogen-acetylated derivatization of the drugs. Confiscated pills, already tentatively identified by some previous procedure, were analyzed by the use of infrared spectroscopy, ultraviolet spectroscopy, and gas chromatography. The same drugs were confirmed by analyzing the alcohols obtained after reductive fragmentation with LiA1H4 as well as the acetylated derivative fractions. Infrared spectra were determined from salt plate films after the evaporation of acidic or basic chloroform extracts of the pill; similarly, ultraviolet spectra were obtained by processing each drug in acidic and basic aqueous solutions. Gas chromatographic analyses involved the determination of characteristic retention times as a function of temperature and the preparation of, and determination of retention times for, derivatives of the drugs. No procedures were specifically included to remove filler material, save its sparing solubility in the extracting solvents; likewise, no special attempts were made to perfect special microtechniques which would allow all analyses to be performed on a single pill. Desired, rather, were the ease, simplicity, and relative speed realized from analysis of approximately five confiscated pills.", "contents": "Identification of drugs and their derivatives. This paper represents a brief review of traditional procedures for the analysis of confiscated drugs and the application of these procedures to the products formed after reductive fragmentation and halogen-acetylated derivatization of the drugs. Confiscated pills, already tentatively identified by some previous procedure, were analyzed by the use of infrared spectroscopy, ultraviolet spectroscopy, and gas chromatography. The same drugs were confirmed by analyzing the alcohols obtained after reductive fragmentation with LiA1H4 as well as the acetylated derivative fractions. Infrared spectra were determined from salt plate films after the evaporation of acidic or basic chloroform extracts of the pill; similarly, ultraviolet spectra were obtained by processing each drug in acidic and basic aqueous solutions. Gas chromatographic analyses involved the determination of characteristic retention times as a function of temperature and the preparation of, and determination of retention times for, derivatives of the drugs. No procedures were specifically included to remove filler material, save its sparing solubility in the extracting solvents; likewise, no special attempts were made to perfect special microtechniques which would allow all analyses to be performed on a single pill. Desired, rather, were the ease, simplicity, and relative speed realized from analysis of approximately five confiscated pills.", "PMID": 956744}
{"id": "pubmed23n0032_2375", "title": "Contaminants in illicit amphetamine preparations.", "content": "Samples from illicit preparations of amphetamine and methamphetamine were investigated by combined gas chromatography-mass spectrometry (GC/MS) to detect and identify contaminants, excipients, and by-products from manufacture. In addition, these samples were subjected to energy-dispersive X-ray (EDX), and it was determined that the preparations of these compounds were made in the present of zinc, probably as a reductant. In addition to identifying polycondensation products from these preparations, the illicit origin of amphetamine-type drugs can generally be determined by the detection of contaminants and by-products present in submitted samples. Thus, a multidisciplinary analytical approach yields data of potential value for comparative examinations and possibly for legal purposes.", "contents": "Contaminants in illicit amphetamine preparations. Samples from illicit preparations of amphetamine and methamphetamine were investigated by combined gas chromatography-mass spectrometry (GC/MS) to detect and identify contaminants, excipients, and by-products from manufacture. In addition, these samples were subjected to energy-dispersive X-ray (EDX), and it was determined that the preparations of these compounds were made in the present of zinc, probably as a reductant. In addition to identifying polycondensation products from these preparations, the illicit origin of amphetamine-type drugs can generally be determined by the detection of contaminants and by-products present in submitted samples. Thus, a multidisciplinary analytical approach yields data of potential value for comparative examinations and possibly for legal purposes.", "PMID": 956745}
{"id": "pubmed23n0032_2376", "title": "Comparative postmortem chemistries of vitreous humor before and after embalming.", "content": "Examination for common biochemical substances in the vitreous humor of embalmed bodies indicates that individuals with significant nitrogen retention or diabetics with marked elevation in vitreous glucose will be recognized by standard laboratory procedures on postembalming specimens. The work of Scott et al [3] on vitreous alcohol in the embalmed body has been substantiated with good general agreement found between specimens obtained before and after the embalming process.", "contents": "Comparative postmortem chemistries of vitreous humor before and after embalming. Examination for common biochemical substances in the vitreous humor of embalmed bodies indicates that individuals with significant nitrogen retention or diabetics with marked elevation in vitreous glucose will be recognized by standard laboratory procedures on postembalming specimens. The work of Scott et al [3] on vitreous alcohol in the embalmed body has been substantiated with good general agreement found between specimens obtained before and after the embalming process.", "PMID": 956746}
{"id": "pubmed23n0032_2377", "title": "Latent fingerprints on cartridges and expended cartridge casings.", "content": "The effects of time and detonation on latent fingerprints from cartridges and cartridge castings were explored. Time alone did not appear to seriously degrade latent fingerprint quality over the three-week period of experimental trials. The greatest effect of detonation seemed to stem from hot gaseous blowback on the external surface of the cartridge casings. Nickel-plated casings, because of their greater chemical resistance and more polished surface, were found less satisfactory as a substrate for latent impressions than brass casings. An interesting technique for development and permanent preservation of latent fingerprint impressions on castridge casings was also described.", "contents": "Latent fingerprints on cartridges and expended cartridge casings. The effects of time and detonation on latent fingerprints from cartridges and cartridge castings were explored. Time alone did not appear to seriously degrade latent fingerprint quality over the three-week period of experimental trials. The greatest effect of detonation seemed to stem from hot gaseous blowback on the external surface of the cartridge casings. Nickel-plated casings, because of their greater chemical resistance and more polished surface, were found less satisfactory as a substrate for latent impressions than brass casings. An interesting technique for development and permanent preservation of latent fingerprint impressions on castridge casings was also described.", "PMID": 956747}
{"id": "pubmed23n0032_2378", "title": "Detection of gunshot residue by use of the scanning electron microscope.", "content": "Particle analysis techniques provide much more information useful for identification of gunshot residue than the conventional analytical techniques that measure only the concentration of elements averaged over the entire specimen. By combining the morphological information by microscopy with elemental analysis by X-ray fluorescence, the SEM provides definitive identification of residue particles. Therefore, the particle analysis technique should be more revealing in situations where conventional methods fail as the quantity of residue approaches the background level.", "contents": "Detection of gunshot residue by use of the scanning electron microscope. Particle analysis techniques provide much more information useful for identification of gunshot residue than the conventional analytical techniques that measure only the concentration of elements averaged over the entire specimen. By combining the morphological information by microscopy with elemental analysis by X-ray fluorescence, the SEM provides definitive identification of residue particles. Therefore, the particle analysis technique should be more revealing in situations where conventional methods fail as the quantity of residue approaches the background level.", "PMID": 956748}
{"id": "pubmed23n0032_2379", "title": "The application of a standard color coding system to paint in forensic science.", "content": "The procedure described for the measurement of paint colors represents an attempt to standardize the measurement of color in the forensic science service and provides a means of communicating colors between individuals. The effect of sample size on color comparisons has been discussed. It has been shown that color data collected from many control samples of paint flakes can provide the frequency of occurrence of paint colors. The errors involved in color comparisons have been assessed and the system has been shown to be a practicable one.", "contents": "The application of a standard color coding system to paint in forensic science. The procedure described for the measurement of paint colors represents an attempt to standardize the measurement of color in the forensic science service and provides a means of communicating colors between individuals. The effect of sample size on color comparisons has been discussed. It has been shown that color data collected from many control samples of paint flakes can provide the frequency of occurrence of paint colors. The errors involved in color comparisons have been assessed and the system has been shown to be a practicable one.", "PMID": 956751}
{"id": "pubmed23n0032_2380", "title": "Unusual three-dimensional bite mark evidence in a homicide case.", "content": "A comparison has been made between bite marks observed on the nose of a female homicide victim and the configuration of the dental structures of a male suspect. The victim's bite marks showed a number of demonstrable characteristics which could be reproduced by models of the dentition of the suspect. Special peculiarities within and between individual teeth, together with the upper and lower jaw relationship, were demonstrably consistent with the bite mark patterns on the victim's nose. Detailed examination of the scientific evidence led the dental investigators to the conclusion that there was a positive match between the suspect's dentition and the bite mark on the decedent's nose. The dental investigation and subsequent dental testimony were prime factors leading to the defendant's conviction of manslaughter in the first case in California involving the major use of bite mark evidence.", "contents": "Unusual three-dimensional bite mark evidence in a homicide case. A comparison has been made between bite marks observed on the nose of a female homicide victim and the configuration of the dental structures of a male suspect. The victim's bite marks showed a number of demonstrable characteristics which could be reproduced by models of the dentition of the suspect. Special peculiarities within and between individual teeth, together with the upper and lower jaw relationship, were demonstrably consistent with the bite mark patterns on the victim's nose. Detailed examination of the scientific evidence led the dental investigators to the conclusion that there was a positive match between the suspect's dentition and the bite mark on the decedent's nose. The dental investigation and subsequent dental testimony were prime factors leading to the defendant's conviction of manslaughter in the first case in California involving the major use of bite mark evidence.", "PMID": 956753}
{"id": "pubmed23n0032_2381", "title": "A suicidal gunshot wound of the back.", "content": "An 18-year-old woman committed suicide by shooting herself in the right temple, presternal region, and lower thoracic back. The anatomic and other investigative data on this case are reported and discussed in the perspective of our experience with suicides by firearms.", "contents": "A suicidal gunshot wound of the back. An 18-year-old woman committed suicide by shooting herself in the right temple, presternal region, and lower thoracic back. The anatomic and other investigative data on this case are reported and discussed in the perspective of our experience with suicides by firearms.", "PMID": 956755}
{"id": "pubmed23n0032_2382", "title": "Heat stroke and fluphenazine therapy: report of a case.", "content": "A fatal case of heat stroke in a 21-year-old man with chronic schizophrenia is reported. Phenothiazine therapy is thought to have been a factor of major importance in the induction of this syndrome. These cases may become the responsibility of the medical examiner, and the importance of obtaining a complete drug history is stressed.", "contents": "Heat stroke and fluphenazine therapy: report of a case. A fatal case of heat stroke in a 21-year-old man with chronic schizophrenia is reported. Phenothiazine therapy is thought to have been a factor of major importance in the induction of this syndrome. These cases may become the responsibility of the medical examiner, and the importance of obtaining a complete drug history is stressed.", "PMID": 956756}
{"id": "pubmed23n0032_2383", "title": "Sodium channel selectivity. Dependence on internal permeant ion concentration.", "content": "The selectivity of sodium channels in squid axon membranes was investigated with widely varying concentrations of internal ions. The selectivity ratio, PNa/PK, determined from reversal potentials decreases from 12.8 to 5.7 to 3.5 as the concentration of internal potassium is reduced from 530 to 180 to 50 mM, respectively. The internal KF perfusion medium can be diluted by tetramethylammonium (TMA), Tris, or sucrose solutions with the same decrease in PNa/PK. The changes in the selectivity ratio depend upon internal permeant ion concentration rather than ionic strength, membrane potential, or chloride permeability. Lowering the internal concentration of cesium, rubidium, guanidnium, or ammonium also reduces PNa/Pion. The selective sequence of the sodium channel is: Na greater than guanidinium greater than ammonium greater than K greater than Rb greater than Cs.", "contents": "Sodium channel selectivity. Dependence on internal permeant ion concentration. The selectivity of sodium channels in squid axon membranes was investigated with widely varying concentrations of internal ions. The selectivity ratio, PNa/PK, determined from reversal potentials decreases from 12.8 to 5.7 to 3.5 as the concentration of internal potassium is reduced from 530 to 180 to 50 mM, respectively. The internal KF perfusion medium can be diluted by tetramethylammonium (TMA), Tris, or sucrose solutions with the same decrease in PNa/PK. The changes in the selectivity ratio depend upon internal permeant ion concentration rather than ionic strength, membrane potential, or chloride permeability. Lowering the internal concentration of cesium, rubidium, guanidnium, or ammonium also reduces PNa/Pion. The selective sequence of the sodium channel is: Na greater than guanidinium greater than ammonium greater than K greater than Rb greater than Cs.", "PMID": 956766}
{"id": "pubmed23n0032_2384", "title": "Water and nonelectrolyte permeability of lipid bilayer membranes.", "content": "Both the permeability coefficients (Pd's) through lipid bilayer membranes of varying composition (lecithin [L], lecithin:cholesterol [LC], and spingomyelin:cholesterol [SC]) and the n-hexadecane:water partition coefficients (Knc's) of H2O and seven nonelectrolytes (1,6 hexanediol, 1,4 butanediol, n-butyramide, isobutyramide, acetamide, formamide, and urea) were measured. For a given membrane compositiin, Pd/DKnc (where D is the diffusion constant in water) is the same for most of the molecules tested. There is no extraordinary dependence of Pd on molecular weight; thus, given Pd(acetamide), Pd(1,6 hexanediol) is correctly predicted from the Knc and D values for the two molecules. The major exceptions are H2O, whose value of Pd/DKnc is about 10-fold larger, and urea, whose value is about 5-fold smaller than the general average. In a \"tight\" membrane such as SC, Pd(n-butyramide)/Pd(isobutyramide)=2.5; thus this bilayer manifests the same sort of discrimination between branched and straight chain molecules as occurs in many plasma membranes. Although the absolute values of the Pd's change by more than a factor of 100 in going from the tightest membrane (SC) to the loosest (L), the relative values remain approximately constant. The general conclusion of this study is that H2O and nonelectrolytes cross lipid bilayer membranes by a solubility-diffusion mechanism, and that the bilayer interior is much more like an oil (a la Overton) than a rubber-like polymer (a la Lieb and Stein).", "contents": "Water and nonelectrolyte permeability of lipid bilayer membranes. Both the permeability coefficients (Pd's) through lipid bilayer membranes of varying composition (lecithin [L], lecithin:cholesterol [LC], and spingomyelin:cholesterol [SC]) and the n-hexadecane:water partition coefficients (Knc's) of H2O and seven nonelectrolytes (1,6 hexanediol, 1,4 butanediol, n-butyramide, isobutyramide, acetamide, formamide, and urea) were measured. For a given membrane compositiin, Pd/DKnc (where D is the diffusion constant in water) is the same for most of the molecules tested. There is no extraordinary dependence of Pd on molecular weight; thus, given Pd(acetamide), Pd(1,6 hexanediol) is correctly predicted from the Knc and D values for the two molecules. The major exceptions are H2O, whose value of Pd/DKnc is about 10-fold larger, and urea, whose value is about 5-fold smaller than the general average. In a \"tight\" membrane such as SC, Pd(n-butyramide)/Pd(isobutyramide)=2.5; thus this bilayer manifests the same sort of discrimination between branched and straight chain molecules as occurs in many plasma membranes. Although the absolute values of the Pd's change by more than a factor of 100 in going from the tightest membrane (SC) to the loosest (L), the relative values remain approximately constant. The general conclusion of this study is that H2O and nonelectrolytes cross lipid bilayer membranes by a solubility-diffusion mechanism, and that the bilayer interior is much more like an oil (a la Overton) than a rubber-like polymer (a la Lieb and Stein).", "PMID": 956767}
{"id": "pubmed23n0032_2385", "title": "Nature of the water permeability increase induced by antidiuretic hormone (ADH) in toad urinary bladder and related tissues.", "content": "In artificial  lipid bilayer membranes, the ratio of the water permeability coefficient (Pd(water)) to the permeability coefficient of an arbitrary nonelectrolyte such as n-butyramide (Pd(n-butyramide)) remains relatively constant with changes in lipid composition and temperature, even though the individual Pd's increase more than 100-fold. I propose that this is a general rule that also holds for the lipid bilayers of cells and tissues, and that therefore if Pd(water)/Pd(solute greatly exceeds the value found for artifical lipid bilayers (where \"solute\" is a molecule, such as 1,6 hexanediol or n-butyramide, that crosses the cell membrane by a solubility-diffusion mechanism without the aid of a special transporting system), then water crosses the cell membrane via aqueous pores. Applying this criterion to the toad urinary bladder, we find that even in the unstimulated bladder, water probably crosses the luminal membrane primarily through small aqueous pores, and that this almost certainly the case after antidiuretic hormone (ADH) stimulation. I suggest that ADH stimulation ultimately leads either to formation (or enlargement) of pores, by the rearrangement of preexisting subunits, or to an unplugging of these pores.", "contents": "Nature of the water permeability increase induced by antidiuretic hormone (ADH) in toad urinary bladder and related tissues. In artificial  lipid bilayer membranes, the ratio of the water permeability coefficient (Pd(water)) to the permeability coefficient of an arbitrary nonelectrolyte such as n-butyramide (Pd(n-butyramide)) remains relatively constant with changes in lipid composition and temperature, even though the individual Pd's increase more than 100-fold. I propose that this is a general rule that also holds for the lipid bilayers of cells and tissues, and that therefore if Pd(water)/Pd(solute greatly exceeds the value found for artifical lipid bilayers (where \"solute\" is a molecule, such as 1,6 hexanediol or n-butyramide, that crosses the cell membrane by a solubility-diffusion mechanism without the aid of a special transporting system), then water crosses the cell membrane via aqueous pores. Applying this criterion to the toad urinary bladder, we find that even in the unstimulated bladder, water probably crosses the luminal membrane primarily through small aqueous pores, and that this almost certainly the case after antidiuretic hormone (ADH) stimulation. I suggest that ADH stimulation ultimately leads either to formation (or enlargement) of pores, by the rearrangement of preexisting subunits, or to an unplugging of these pores.", "PMID": 956768}
{"id": "pubmed23n0032_2386", "title": "Axoplasmic free magnesium levels and magnesium extrusion from squid giant axons.", "content": "The free magnesium concentration in the axoplasm of the giant axon of the squid, Loligo pealei, was estimated by exploting the known sensitivity of the sodium pump to intracellular Mg2+ levels. The Mg-citrate buffer which, when injected into the axon, resulted in no change in sodium efflux was in equilibrium with a Mg2+ level of about 3--4 mM. Optimal [Mg2+] for the sodium pump is somewhat higher. Total magnesium content of axoplasm was 6.7 mmol/kg, and that of hemolymph was 44 mM. The rate coefficient for 28Mg efflux was about 2 X 10(-3) min-u for a 500-mum axon at 22-25degreesC, with a very high temperature coefficient (Q10=4-5). This efflux is inhibited 95% by injection of apyrase and 75% by removal of external sodium, and seems unaffected by membrane potential or potassium ions. Increased intracellular ADP levels do not affect Mg efflux nor its requirement for Na+/o, but extracellularl magnesium ions do. Activation of 28Mg efflux by Na+/o follows hyperbolic kinetics, with Mg2+/o reducing the affinity of the system for Na+/o. Lanthanum and D600 reversibly inhibit Mg efflux. In the absence of both Na+ and Mg2+, but not in their presence, removal of Ca2+ from the seawater vastly increased 28Mg efflux; this efflux was also strongly inhibited by lanthanum. A small (10(-14) mol cm-2) extra Mg efflux accompanies the conduction of an action potential.", "contents": "Axoplasmic free magnesium levels and magnesium extrusion from squid giant axons. The free magnesium concentration in the axoplasm of the giant axon of the squid, Loligo pealei, was estimated by exploting the known sensitivity of the sodium pump to intracellular Mg2+ levels. The Mg-citrate buffer which, when injected into the axon, resulted in no change in sodium efflux was in equilibrium with a Mg2+ level of about 3--4 mM. Optimal [Mg2+] for the sodium pump is somewhat higher. Total magnesium content of axoplasm was 6.7 mmol/kg, and that of hemolymph was 44 mM. The rate coefficient for 28Mg efflux was about 2 X 10(-3) min-u for a 500-mum axon at 22-25degreesC, with a very high temperature coefficient (Q10=4-5). This efflux is inhibited 95% by injection of apyrase and 75% by removal of external sodium, and seems unaffected by membrane potential or potassium ions. Increased intracellular ADP levels do not affect Mg efflux nor its requirement for Na+/o, but extracellularl magnesium ions do. Activation of 28Mg efflux by Na+/o follows hyperbolic kinetics, with Mg2+/o reducing the affinity of the system for Na+/o. Lanthanum and D600 reversibly inhibit Mg efflux. In the absence of both Na+ and Mg2+, but not in their presence, removal of Ca2+ from the seawater vastly increased 28Mg efflux; this efflux was also strongly inhibited by lanthanum. A small (10(-14) mol cm-2) extra Mg efflux accompanies the conduction of an action potential.", "PMID": 956769}
{"id": "pubmed23n0032_2387", "title": "Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.", "content": "It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.", "contents": "Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica. It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.", "PMID": 956770}
{"id": "pubmed23n0032_2388", "title": "Precursor and product processing in the bag cell neurons of Aplysia californica.", "content": "Posttranslational processing in the biosynthesis of the egg-laying hormone (ELH) by the bag cell neurons of Aplysia californica was studied. The precursor (pro-ELH) to ELH was found to be resistant to solubilization in denaturant-free media throughout its lifetime. Its principle products show a similar insolubility for 3 h, but two of these, ca. 6,000 daltons, subsequently become readily recoverable in the low-speed supernatant of a homogenate of the cells. The remaining product shows no change in solubility characteristics. From studies employing ultracentrifugation and examination of axoplasmic transport, the solubility shift for the lower molecular weight products is interpreted to represent the liberation of secretory vesicles into the cytoplasm from larger membranous associations. This event is accompanied by, but does appear to be dependent upon, a 15% reduction in the molecular weight of one of the products. These findings are considered in the light of the extensively studied posttranslational processing regimen for the production of insulin in the pancreatic beta cell.", "contents": "Precursor and product processing in the bag cell neurons of Aplysia californica. Posttranslational processing in the biosynthesis of the egg-laying hormone (ELH) by the bag cell neurons of Aplysia californica was studied. The precursor (pro-ELH) to ELH was found to be resistant to solubilization in denaturant-free media throughout its lifetime. Its principle products show a similar insolubility for 3 h, but two of these, ca. 6,000 daltons, subsequently become readily recoverable in the low-speed supernatant of a homogenate of the cells. The remaining product shows no change in solubility characteristics. From studies employing ultracentrifugation and examination of axoplasmic transport, the solubility shift for the lower molecular weight products is interpreted to represent the liberation of secretory vesicles into the cytoplasm from larger membranous associations. This event is accompanied by, but does appear to be dependent upon, a 15% reduction in the molecular weight of one of the products. These findings are considered in the light of the extensively studied posttranslational processing regimen for the production of insulin in the pancreatic beta cell.", "PMID": 956771}
{"id": "pubmed23n0032_2389", "title": "Genetic studies on SCP1-prime strains of Streptomyces coelicolor A3(2).", "content": "SCP1'-cysB carries a short chromosomal insertion: cysB was the only chromosomal locus identified on this plasmid; the plasmid was seldom lost; and a comparatively low frequency of crossing-over between the exo- and endogenote in a cysB+/cysB+ heterozygote. SCP1'-argA, uraB carries a longer chromosomal insertion: five chromosomal loci have been detected on the plasmid; it was very often lost; and there was frequent crossing-over between exo- and endogenote in heterozygous strains. Both SCP1' plasmids gave rise to plasmids closely resembling, or perhaps identical with, the original SCP1 by deletion of the chromosomal insertion. Markers argA and uraB were always deleted together from SCP1'-argA, uraB, whereas random deletion should more often have removed uraB alone. This suggests that special DNA sequences are involved in SCP1' formation. An analysis of recombination between exo- and endogenote within a double heterozygote for argA and uraB supports the hypothesis that single copies of plasmid and chromosome assort from a pool of interacting molecules at, or immediately before, spore formation. A strain carrying the SCP1'-cysB plasmid gave rise to a 'bidirectional' donor of chromosomal markers in which the plasmid had apparently integrated into the chromosome at or close to the cysB locus.", "contents": "Genetic studies on SCP1-prime strains of Streptomyces coelicolor A3(2). SCP1'-cysB carries a short chromosomal insertion: cysB was the only chromosomal locus identified on this plasmid; the plasmid was seldom lost; and a comparatively low frequency of crossing-over between the exo- and endogenote in a cysB+/cysB+ heterozygote. SCP1'-argA, uraB carries a longer chromosomal insertion: five chromosomal loci have been detected on the plasmid; it was very often lost; and there was frequent crossing-over between exo- and endogenote in heterozygous strains. Both SCP1' plasmids gave rise to plasmids closely resembling, or perhaps identical with, the original SCP1 by deletion of the chromosomal insertion. Markers argA and uraB were always deleted together from SCP1'-argA, uraB, whereas random deletion should more often have removed uraB alone. This suggests that special DNA sequences are involved in SCP1' formation. An analysis of recombination between exo- and endogenote within a double heterozygote for argA and uraB supports the hypothesis that single copies of plasmid and chromosome assort from a pool of interacting molecules at, or immediately before, spore formation. A strain carrying the SCP1'-cysB plasmid gave rise to a 'bidirectional' donor of chromosomal markers in which the plasmid had apparently integrated into the chromosome at or close to the cysB locus.", "PMID": 956772}
{"id": "pubmed23n0032_2390", "title": "Synthesis and hydrolysis of malyl-coenzyme A by Pseudomonas AM1: an apparent malate synthase activity.", "content": "The malate synthase activity detectable in crude extracts of Pseudomonas AM1 has been shown to be due to a coupling of a malyl-CoA hydrolase with malyl-CoA lyase and not due to a discrete malate synthase enzyme. The partial purification of this malyl-CoA hydrolase from Pseudomonas AM1 has shown that it is distinct from citrate synthase which also hydrolyses malyl-CoA. The malyl-CoA hydrolase has a low Km for malyl-CoA (7-0 muM). A mutant of Pseudomonas AM1, ICT51 (Taylor & Anthony, 1975), which is unable to grow on ethanol, malonate or 3-hydroxybutyrate, has been shown to have an altered malyl-CoA hydrolase with a Km for malyl-CoA 30 times higher than that of the enzyme present in the wild-type organism. Two classes of revertants to growth on these substrates have been isolated: (i) those with a malyl-CoA hydrolase of similar Km to the wild-type and (ii) those in which the malyl-CoA hydrolase activity remains the same as in the mutant ICT51. The nature of the mutation leading to the latter class of revertants is unknown.", "contents": "Synthesis and hydrolysis of malyl-coenzyme A by Pseudomonas AM1: an apparent malate synthase activity. The malate synthase activity detectable in crude extracts of Pseudomonas AM1 has been shown to be due to a coupling of a malyl-CoA hydrolase with malyl-CoA lyase and not due to a discrete malate synthase enzyme. The partial purification of this malyl-CoA hydrolase from Pseudomonas AM1 has shown that it is distinct from citrate synthase which also hydrolyses malyl-CoA. The malyl-CoA hydrolase has a low Km for malyl-CoA (7-0 muM). A mutant of Pseudomonas AM1, ICT51 (Taylor & Anthony, 1975), which is unable to grow on ethanol, malonate or 3-hydroxybutyrate, has been shown to have an altered malyl-CoA hydrolase with a Km for malyl-CoA 30 times higher than that of the enzyme present in the wild-type organism. Two classes of revertants to growth on these substrates have been isolated: (i) those with a malyl-CoA hydrolase of similar Km to the wild-type and (ii) those in which the malyl-CoA hydrolase activity remains the same as in the mutant ICT51. The nature of the mutation leading to the latter class of revertants is unknown.", "PMID": 956773}
{"id": "pubmed23n0032_2391", "title": "Existence of a surface configuration on the aerial spore and aerial mycelium of Micropolyspora.", "content": "We report the existence of a surface configuration in a true spore-forming genus having cell wall constituents of type IV. Micropolyspora angiospora, M. caesia and M. faeni freeze-fractured along the wall surface and only had a surface configuration on their aerial mycelium and aerial spores, with none on the substrate mycelium and substrate spore. The surface configuration of Micropolyspora was distinctly more complicated than that of Nocardia. The aerial spores of M. angiospora were characteristic in that they possessed ridges, two kinds of surface configurations (i.e. rodlets and fibres), and a complex pattern on the surface. Some rodlets of this organism were formed of a two-stranded helix, each strand having a diameter similar to that of a fibre.", "contents": "Existence of a surface configuration on the aerial spore and aerial mycelium of Micropolyspora. We report the existence of a surface configuration in a true spore-forming genus having cell wall constituents of type IV. Micropolyspora angiospora, M. caesia and M. faeni freeze-fractured along the wall surface and only had a surface configuration on their aerial mycelium and aerial spores, with none on the substrate mycelium and substrate spore. The surface configuration of Micropolyspora was distinctly more complicated than that of Nocardia. The aerial spores of M. angiospora were characteristic in that they possessed ridges, two kinds of surface configurations (i.e. rodlets and fibres), and a complex pattern on the surface. Some rodlets of this organism were formed of a two-stranded helix, each strand having a diameter similar to that of a fibre.", "PMID": 956774}
{"id": "pubmed23n0032_2392", "title": "Phytochrome system of the yeast Candida guilliermondii and recovery from ultraviolet injury.", "content": "The effect of red (660 nm) and far-red (730 nm) light on the stability of the yeast Candida guilliermondii to lethal u.v. radiation has been studied. Reactivation and protection were exhibited for 30 min after treatment with red light and were abolished by far-red exposure applied within this time period. The temperature dependence of the reactivation effect was also studied. The data obtained showed that the properties of recovery and protection against u.v. exposure are associated with the phytochrome system of the yeast.", "contents": "Phytochrome system of the yeast Candida guilliermondii and recovery from ultraviolet injury. The effect of red (660 nm) and far-red (730 nm) light on the stability of the yeast Candida guilliermondii to lethal u.v. radiation has been studied. Reactivation and protection were exhibited for 30 min after treatment with red light and were abolished by far-red exposure applied within this time period. The temperature dependence of the reactivation effect was also studied. The data obtained showed that the properties of recovery and protection against u.v. exposure are associated with the phytochrome system of the yeast.", "PMID": 956776}
{"id": "pubmed23n0032_2393", "title": "Isolation and structure of an extracellular polysaccharide from streptomyces sp. FERM-P1185.", "content": "A streptomycete strain (FERM-P1185), isolated from soil, secreted a slime on glucose-asparagine agar, and produced viscous growth in liquid media containing peptone as nitrogen source. A purified polysaccharide isolated from the culture broth was composed of glucose and mannose units (molar ratio 1-87:1). Periodate oxidation, Smith degradation, methylation analysis, infrared and 13c nuclear magnetic resonance spectra indicated that this mannoglucan had a linear structure consisting of alpha-1,3- and alpha-1,4-linked glucopyranose and mannopyranose units.", "contents": "Isolation and structure of an extracellular polysaccharide from streptomyces sp. FERM-P1185. A streptomycete strain (FERM-P1185), isolated from soil, secreted a slime on glucose-asparagine agar, and produced viscous growth in liquid media containing peptone as nitrogen source. A purified polysaccharide isolated from the culture broth was composed of glucose and mannose units (molar ratio 1-87:1). Periodate oxidation, Smith degradation, methylation analysis, infrared and 13c nuclear magnetic resonance spectra indicated that this mannoglucan had a linear structure consisting of alpha-1,3- and alpha-1,4-linked glucopyranose and mannopyranose units.", "PMID": 956777}
{"id": "pubmed23n0032_2394", "title": "Effect of light on growth and sporulation of Aspergillus ornatus.", "content": "Aspergillus ornatus produces conidia when grown in continuous light but few, if any, when grown in continuous darkness. A minimum of 3 h of exposure to light is needed for induction. Light inhibits growth, glucose uptake and phosphorylation but does not inhibit the uptake of lysine. A low molecular weight substance produced or accumulating in the light inhibits the phosphorylation of glucose. It is suggested that the inhibition of glucose uptake and phosphorylation precedes conidiation and that conidiation may be the result of starvation caused by this light-induced inhibition.", "contents": "Effect of light on growth and sporulation of Aspergillus ornatus. Aspergillus ornatus produces conidia when grown in continuous light but few, if any, when grown in continuous darkness. A minimum of 3 h of exposure to light is needed for induction. Light inhibits growth, glucose uptake and phosphorylation but does not inhibit the uptake of lysine. A low molecular weight substance produced or accumulating in the light inhibits the phosphorylation of glucose. It is suggested that the inhibition of glucose uptake and phosphorylation precedes conidiation and that conidiation may be the result of starvation caused by this light-induced inhibition.", "PMID": 956778}
{"id": "pubmed23n0032_2395", "title": "Ultrastructure of Rothia dentocariosa.", "content": "Rothia dentocariosa was seen as a typical prokaryotic cell, lacking nuclear envelope, mitochondria and a reticulum with ribosomes. The plasma membrane was located close to and parallel to the wall. The outer limits of the wall were associated with what may be capsular or slime material. Chain-like filaments of thick walled coccoid cells underwent septation both transverse and parallel to the long axis of the chain. Side branching and terminal clavate forms were also present. These clavate forms may represent specialized cells during the life-cycle. Fragmentation of the chain resulted when the outer wall ruptured to release the coccoid bodies.", "contents": "Ultrastructure of Rothia dentocariosa. Rothia dentocariosa was seen as a typical prokaryotic cell, lacking nuclear envelope, mitochondria and a reticulum with ribosomes. The plasma membrane was located close to and parallel to the wall. The outer limits of the wall were associated with what may be capsular or slime material. Chain-like filaments of thick walled coccoid cells underwent septation both transverse and parallel to the long axis of the chain. Side branching and terminal clavate forms were also present. These clavate forms may represent specialized cells during the life-cycle. Fragmentation of the chain resulted when the outer wall ruptured to release the coccoid bodies.", "PMID": 956779}
{"id": "pubmed23n0032_2396", "title": "Butyricin 7423: a bacteriocin produced by Clostridium butyricum NCIB7423.", "content": "Butyricin 7423 is a trypsin-sensitive bacteriocin produced by Clostridium butyricum NCIB7423 and active against some other species of Clostridium. It has a bactericidal but non-lytic action on growing cultures of Clostridium pasteurianum. The primary action of butyricin 7423 appears to be at the cell membrane. Thus treatment of C. pasteurianum with an excess of butyricin altered the permeability of its cell membrane, allowing the release of several metabolites and ions. Efflux of rubidium ions from organisms preloaded with 86Rb+ was particularly rapid and extensive. The action of butyricin 7423 on Clostridium species seems to differ from that of other clostridocins, such as the boticins and perfringocin 28, and more closely resembles the mode of action on susceptible (aerobic) organisms of bacteriocins such as staphylococcin 1580 or colicins A, E1, I and K.", "contents": "Butyricin 7423: a bacteriocin produced by Clostridium butyricum NCIB7423. Butyricin 7423 is a trypsin-sensitive bacteriocin produced by Clostridium butyricum NCIB7423 and active against some other species of Clostridium. It has a bactericidal but non-lytic action on growing cultures of Clostridium pasteurianum. The primary action of butyricin 7423 appears to be at the cell membrane. Thus treatment of C. pasteurianum with an excess of butyricin altered the permeability of its cell membrane, allowing the release of several metabolites and ions. Efflux of rubidium ions from organisms preloaded with 86Rb+ was particularly rapid and extensive. The action of butyricin 7423 on Clostridium species seems to differ from that of other clostridocins, such as the boticins and perfringocin 28, and more closely resembles the mode of action on susceptible (aerobic) organisms of bacteriocins such as staphylococcin 1580 or colicins A, E1, I and K.", "PMID": 956780}
{"id": "pubmed23n0032_2397", "title": "Nitrogen fixation by bacteria from the hindgut of termites.", "content": "Anaerobically grown bacteria isolated from the hindgut contents of the termites Coptotermes lacteus (Froggatt), Mastotermes darwiniensis Froggatt and Nasutitermes exitiosus (Hill) were nitrogenase-positive as assayed by acetylene reduction. Nitrogen fixation, confirmed with 15N2, was highest in the isolate from M. darwiniensis. All isolates were identified as Citrobacter freundii (Braak) Werkman & Gillen.", "contents": "Nitrogen fixation by bacteria from the hindgut of termites. Anaerobically grown bacteria isolated from the hindgut contents of the termites Coptotermes lacteus (Froggatt), Mastotermes darwiniensis Froggatt and Nasutitermes exitiosus (Hill) were nitrogenase-positive as assayed by acetylene reduction. Nitrogen fixation, confirmed with 15N2, was highest in the isolate from M. darwiniensis. All isolates were identified as Citrobacter freundii (Braak) Werkman & Gillen.", "PMID": 956782}
{"id": "pubmed23n0032_2398", "title": "An approach to numerical identification of bacterial species.", "content": "The distribution of matching coefficients (M values) for strains of a species to their own 'hypothetical mean organism' (HMO) or to HMO patterns of other organisms was studied in 754 strains of 19 mycobacterial species, testing for 91 discriminating characters. The M values of strains of a species to the HMO of other species usually showed a normal distribution, and M values to their own HMO showed either a normal distribution of a binomial distribution, depending on the mean of M values. If the number of test characters was large, the binomial distribution usually resembled the normal distribution. After preparation of the HMO for every species and estimation of the mean of the M values (M) and the standard deviation (s), numerical identification could be carried out: if a test strain had an M value to the HMO of species chi that only fell within the range (M +/2s) for species chi, the strain would be identified as a member of that species.", "contents": "An approach to numerical identification of bacterial species. The distribution of matching coefficients (M values) for strains of a species to their own 'hypothetical mean organism' (HMO) or to HMO patterns of other organisms was studied in 754 strains of 19 mycobacterial species, testing for 91 discriminating characters. The M values of strains of a species to the HMO of other species usually showed a normal distribution, and M values to their own HMO showed either a normal distribution of a binomial distribution, depending on the mean of M values. If the number of test characters was large, the binomial distribution usually resembled the normal distribution. After preparation of the HMO for every species and estimation of the mean of the M values (M) and the standard deviation (s), numerical identification could be carried out: if a test strain had an M value to the HMO of species chi that only fell within the range (M +/2s) for species chi, the strain would be identified as a member of that species.", "PMID": 956783}
{"id": "pubmed23n0032_2399", "title": "Synthesis of ribonucleic acids during the germination of Botryodiplodia theobromae pycnidiospores.", "content": "During the initial phases of germination of Botryodiplodia theobromae pycnidiospores, protein synthesis was initiated in the absence of detectable ribonucleic acid (RNA) synthesis. The lack of RNA synthesis during the early stages of germination was not an artefact due to reduced precursor permeability or to changes in the nucleotide pool. Further studies showed that the synthesis of messenger RNA and transfer RNA began about 30 min after the initiation of germination and that of 5S RNA after 45 min, whereas ribosomal RNA synthesis was not detected until after 1 h of germination. Approximately 45 to 55% of the messenger-like RNA synthesized during the first hour of germination was devoid of polyadenylate segments.", "contents": "Synthesis of ribonucleic acids during the germination of Botryodiplodia theobromae pycnidiospores. During the initial phases of germination of Botryodiplodia theobromae pycnidiospores, protein synthesis was initiated in the absence of detectable ribonucleic acid (RNA) synthesis. The lack of RNA synthesis during the early stages of germination was not an artefact due to reduced precursor permeability or to changes in the nucleotide pool. Further studies showed that the synthesis of messenger RNA and transfer RNA began about 30 min after the initiation of germination and that of 5S RNA after 45 min, whereas ribosomal RNA synthesis was not detected until after 1 h of germination. Approximately 45 to 55% of the messenger-like RNA synthesized during the first hour of germination was devoid of polyadenylate segments.", "PMID": 956784}
{"id": "pubmed23n0032_2400", "title": "Virulence heterogeneity of a predominantly avirulent Western equine encephalitis virus population.", "content": "Selective removal of small plaque (SP) Western equine encephalitis (WEE) virus from a population heterogeneous with respect to virulence and plaque morphology permitted direct detection of a small sub-population of virulent large plaque (LP) WEE virus. Selective removal of SP-WEE virus was achieved by intracardiac (i.c.a.) inoculation of hamsters; plasma obtained 60 min after inoculation was proportionately enriched for LP-WEE virus since only the SP-WEE virus was cleared. By this method, the proportion of LP- to SP-WEE virus, in a population of SP-WEE virus which appeared to be homogeneous by conventional plaquing methods, was calculated to be 1 LP- to 250000 SP-WEE virions. The presence of a virulent LP-WEE virus sub-population explains why a single passage of a high but not low dose of SP-WEE virus in hamsters resulted in the emergence of an LP-WEE virus population with enhanced virulence.", "contents": "Virulence heterogeneity of a predominantly avirulent Western equine encephalitis virus population. Selective removal of small plaque (SP) Western equine encephalitis (WEE) virus from a population heterogeneous with respect to virulence and plaque morphology permitted direct detection of a small sub-population of virulent large plaque (LP) WEE virus. Selective removal of SP-WEE virus was achieved by intracardiac (i.c.a.) inoculation of hamsters; plasma obtained 60 min after inoculation was proportionately enriched for LP-WEE virus since only the SP-WEE virus was cleared. By this method, the proportion of LP- to SP-WEE virus, in a population of SP-WEE virus which appeared to be homogeneous by conventional plaquing methods, was calculated to be 1 LP- to 250000 SP-WEE virions. The presence of a virulent LP-WEE virus sub-population explains why a single passage of a high but not low dose of SP-WEE virus in hamsters resulted in the emergence of an LP-WEE virus population with enhanced virulence.", "PMID": 956785}
{"id": "pubmed23n0032_2401", "title": "Suppression of interferon production by vitamin A.", "content": "Vitamin A (retinoic acid) suppressed interferon production by L cells infected with Newcastle disease virus (NDV). This suppression was maximal when cells were treated with retinoic acid for 2 h after NDV adsorption, indicating that the inhibitory step was an early event. It was not due to inhibition of total RNA synthesis. Retinoic acid treatment caused both a delay in appearance of interferon and a reduced rate of synthesis thereafter.", "contents": "Suppression of interferon production by vitamin A. Vitamin A (retinoic acid) suppressed interferon production by L cells infected with Newcastle disease virus (NDV). This suppression was maximal when cells were treated with retinoic acid for 2 h after NDV adsorption, indicating that the inhibitory step was an early event. It was not due to inhibition of total RNA synthesis. Retinoic acid treatment caused both a delay in appearance of interferon and a reduced rate of synthesis thereafter.", "PMID": 956786}
{"id": "pubmed23n0032_2402", "title": "Bacteriophage growth on stationary phase Achromobacter cells.", "content": "A new phage-host system is described in which phage alpha 3a grows on stationary phase Achromobacter mutant strains. Characteristic clear plaques are formed, at an e.o.p. 10(-1) to 10(-2), on already confluent bacterial lawns of the mutant strains. Phage growth is sensitive to aeration and growth only occurs under micro-aerophilic conditions. Lysates prepared on the mutant strains cannot transduce in contrast to transducing lysates prepared from wild type Achromobacter strains.", "contents": "Bacteriophage growth on stationary phase Achromobacter cells. A new phage-host system is described in which phage alpha 3a grows on stationary phase Achromobacter mutant strains. Characteristic clear plaques are formed, at an e.o.p. 10(-1) to 10(-2), on already confluent bacterial lawns of the mutant strains. Phage growth is sensitive to aeration and growth only occurs under micro-aerophilic conditions. Lysates prepared on the mutant strains cannot transduce in contrast to transducing lysates prepared from wild type Achromobacter strains.", "PMID": 956787}
{"id": "pubmed23n0032_2403", "title": "Establishment of a secondary drive based on thirst: a replication.", "content": "In order to establish a secondary drive based on thirst, 16 male albino rats were deprived of water for 23 hours per day for 14 days in square cages. At the end of each 23-hour period, the Ss were placed in triangular cages with free access to water for 1/2 hour followed by 1/2 hour in a circular cage with no water available. On day 15 the Ss were divided into two groups following satiation in the triangular cage. The control Ss were placed in the circular cages with water present for the first time, and experimental Ss were placed in the square cages with water present for the first time. During this 1/2-hour period the experimental Ss drank significantly more than the control Ss which indicated the presence of thirst fear in the experimental group.", "contents": "Establishment of a secondary drive based on thirst: a replication. In order to establish a secondary drive based on thirst, 16 male albino rats were deprived of water for 23 hours per day for 14 days in square cages. At the end of each 23-hour period, the Ss were placed in triangular cages with free access to water for 1/2 hour followed by 1/2 hour in a circular cage with no water available. On day 15 the Ss were divided into two groups following satiation in the triangular cage. The control Ss were placed in the circular cages with water present for the first time, and experimental Ss were placed in the square cages with water present for the first time. During this 1/2-hour period the experimental Ss drank significantly more than the control Ss which indicated the presence of thirst fear in the experimental group.", "PMID": 956788}
{"id": "pubmed23n0032_2404", "title": "Acoustic image in visual detection for deaf and hearing college students.", "content": "To explore the role of acoustic factors in visual detection, this study employed 40 deaf and hearing Ss. Ss were requested to cancel all the letters \"e\" in a passage from Treasure Island. Results were analyzed in terms of probabilities of missing a pronounced or silent e and the e in the word \"the.\" Hearing and hard of hearing Ss were more likely to miss silent e's than pronounced e's. There was no significant difference between silent and pronounced e's for the profoundly deaf. Deaf and hearing Ss missed significantly more e's in \"the\" than pronounced or silent e's. The deaf, when compared to the hearing Ss, were more efficient in detecting pronounced and silent e's. They did not differ significantly from the hearing Ss in detecting the e in \"the.\"", "contents": "Acoustic image in visual detection for deaf and hearing college students. To explore the role of acoustic factors in visual detection, this study employed 40 deaf and hearing Ss. Ss were requested to cancel all the letters \"e\" in a passage from Treasure Island. Results were analyzed in terms of probabilities of missing a pronounced or silent e and the e in the word \"the.\" Hearing and hard of hearing Ss were more likely to miss silent e's than pronounced e's. There was no significant difference between silent and pronounced e's for the profoundly deaf. Deaf and hearing Ss missed significantly more e's in \"the\" than pronounced or silent e's. The deaf, when compared to the hearing Ss, were more efficient in detecting pronounced and silent e's. They did not differ significantly from the hearing Ss in detecting the e in \"the.\"", "PMID": 956789}
{"id": "pubmed23n0032_2405", "title": "An experimental analysis of the Chevreul pendulum illusion.", "content": "An investigation was performed, with the use of 60 male and female college students, to quantify the Chevreul pendulum illusory effect, the tendency of a small pendulum, when suspended from the hand and imaginatively concentrated upon, to oscillate seemingly of its own accord. By means of a time exposure photographic measurement technique, strong parametric influences of the pendulum's sinusoidal motion were isolated. It was found that the pendulum effect was enhanced when (a) attentional capacity remained undivided, (b) the amount of musculature used to suspend the pendulum was at a maximum, (c) oscillating visual and auditory external stimuli were present, and (d) females were Ss. In addition, the visual stimulus was found to be superior to its auditory counterpart. The relevance of ideomotor and visual capture interpretations of covert muscle processes in the pendulum illusion was discussed.", "contents": "An experimental analysis of the Chevreul pendulum illusion. An investigation was performed, with the use of 60 male and female college students, to quantify the Chevreul pendulum illusory effect, the tendency of a small pendulum, when suspended from the hand and imaginatively concentrated upon, to oscillate seemingly of its own accord. By means of a time exposure photographic measurement technique, strong parametric influences of the pendulum's sinusoidal motion were isolated. It was found that the pendulum effect was enhanced when (a) attentional capacity remained undivided, (b) the amount of musculature used to suspend the pendulum was at a maximum, (c) oscillating visual and auditory external stimuli were present, and (d) females were Ss. In addition, the visual stimulus was found to be superior to its auditory counterpart. The relevance of ideomotor and visual capture interpretations of covert muscle processes in the pendulum illusion was discussed.", "PMID": 956790}
{"id": "pubmed23n0032_2406", "title": "Chronic methylphenidate effects on learning in an F2 generation.", "content": "This study investigated chronic methylphenidate effects on the learning of undrugged second generation offspring. Seventy-two albino rats were tested on avoidance conditioning. The results indicated that (a) the learning of the undrugged offspring was affected, (b) differential sex effects were obtained, and (c) some difficulty was encountered in reproduction.", "contents": "Chronic methylphenidate effects on learning in an F2 generation. This study investigated chronic methylphenidate effects on the learning of undrugged second generation offspring. Seventy-two albino rats were tested on avoidance conditioning. The results indicated that (a) the learning of the undrugged offspring was affected, (b) differential sex effects were obtained, and (c) some difficulty was encountered in reproduction.", "PMID": 956791}
{"id": "pubmed23n0032_2407", "title": "Unconditioned response diminution in the skin resistance response.", "content": "An experiment was carried out to investigate unconditioned response diminution in the skin resistance response. By using an alternating series of paired and unpaired stimuli the differential predictions of the hypotheses of response interference, conditioned inhibition and expectancy could be tested. Ss were 27 young adult male students. The unconditioned stimulus was a tone of 105 db. For the conditioned stimulus the Ss were divided into two groups, one receiving a tone at 65 db and the other a tone at 85 db. Unconditioned response diminution was found. This diminution showed no significant change over trials and showed no correlation with amplitude of the conditioned response. This result is best explained in terms of expectancy.", "contents": "Unconditioned response diminution in the skin resistance response. An experiment was carried out to investigate unconditioned response diminution in the skin resistance response. By using an alternating series of paired and unpaired stimuli the differential predictions of the hypotheses of response interference, conditioned inhibition and expectancy could be tested. Ss were 27 young adult male students. The unconditioned stimulus was a tone of 105 db. For the conditioned stimulus the Ss were divided into two groups, one receiving a tone at 65 db and the other a tone at 85 db. Unconditioned response diminution was found. This diminution showed no significant change over trials and showed no correlation with amplitude of the conditioned response. This result is best explained in terms of expectancy.", "PMID": 956792}
{"id": "pubmed23n0032_2408", "title": "Preferences by northern grasshopper mice for solutions of sugars, acids, and salts in Richter-type drinking tests.", "content": "Taste preferences, as measured in 48-hour, Richter-type drinking tests (test solution opposite distilled water), were determined for northern grasshopper mice (Onychomys leucogaster, ssp. breviauritus). The Ss were nine males and nine females which were individually housed within an environmental chamber. The test solutions were prepared from five sugars (fructose, glucose, lactose, maltose, sucrose) three salts (magnesium sulfate, potassium chloride, sodium chloride), and two acids (citric acid and hydrochloric acid). In randomly assigned order, each sugar and each salt solution was presented at five molar concentrations, and each acid was paired with distilled water at six levels of pH. Strong drinking preferences were shown for all concentrations of the sugars above .05-.10 M, and sugars ranked in order of preference as follows: Maltose = sucrose greater than glucose = fructose greater than lactose. Preferences were also shown for hypotonic concentrations of NaCl. The other salts and both acids, however, were indifferently preferred at low concentrations and were rejected at the higher concentrations. Taste preferences by grasshopper mice for these chemicals were similar to those exhibited by Monogolian gerbils tested with the same items. The similar patterns of preference shown by New World cricetid rodents (grasshopper mice) and Old World cricetid rodents (gerbils) suggest that conclusions concerning disparity in taste sensibilities among animal forms may be premature.", "contents": "Preferences by northern grasshopper mice for solutions of sugars, acids, and salts in Richter-type drinking tests. Taste preferences, as measured in 48-hour, Richter-type drinking tests (test solution opposite distilled water), were determined for northern grasshopper mice (Onychomys leucogaster, ssp. breviauritus). The Ss were nine males and nine females which were individually housed within an environmental chamber. The test solutions were prepared from five sugars (fructose, glucose, lactose, maltose, sucrose) three salts (magnesium sulfate, potassium chloride, sodium chloride), and two acids (citric acid and hydrochloric acid). In randomly assigned order, each sugar and each salt solution was presented at five molar concentrations, and each acid was paired with distilled water at six levels of pH. Strong drinking preferences were shown for all concentrations of the sugars above .05-.10 M, and sugars ranked in order of preference as follows: Maltose = sucrose greater than glucose = fructose greater than lactose. Preferences were also shown for hypotonic concentrations of NaCl. The other salts and both acids, however, were indifferently preferred at low concentrations and were rejected at the higher concentrations. Taste preferences by grasshopper mice for these chemicals were similar to those exhibited by Monogolian gerbils tested with the same items. The similar patterns of preference shown by New World cricetid rodents (grasshopper mice) and Old World cricetid rodents (gerbils) suggest that conclusions concerning disparity in taste sensibilities among animal forms may be premature.", "PMID": 956793}
{"id": "pubmed23n0032_2409", "title": "Utilization review, attempted suicide, and involuntary hospitalization.", "content": "A patient's refusal to participate in recommended treatment is a problem faced in all branches of medicine. However, psychiatry faces special problems because of its authority to impose hospitialization of suicide attempters, cases were identified where there was indepdent agreement between both the experts' standards and the treating resident psychiatrists that hospitalization was required. Despite this agreement, the patients were not hospitalized because the patients refused. By most criteria, these patients were a high risk group. They had made repeated suicide attempts, used lethal means which eventuated in serious medical consequences, and were still suicidal when referred for treatment. The emergency room psychiatrists reported feeling confused, anxious, and annoyed in dealing with these patients, and the patients signed out against medical advice. A review of these cases indicated that discussions of social control vs. medical responsibility and clear criteria for hospitalization should be incorporated into residency training programs since the emergency room resident faces these tension-producing issues frequently with several different types of patients. Moreover, utilization review criteria may help to set standards which will assist the psychiatrist in making these difficult decisions.", "contents": "Utilization review, attempted suicide, and involuntary hospitalization. A patient's refusal to participate in recommended treatment is a problem faced in all branches of medicine. However, psychiatry faces special problems because of its authority to impose hospitialization of suicide attempters, cases were identified where there was indepdent agreement between both the experts' standards and the treating resident psychiatrists that hospitalization was required. Despite this agreement, the patients were not hospitalized because the patients refused. By most criteria, these patients were a high risk group. They had made repeated suicide attempts, used lethal means which eventuated in serious medical consequences, and were still suicidal when referred for treatment. The emergency room psychiatrists reported feeling confused, anxious, and annoyed in dealing with these patients, and the patients signed out against medical advice. A review of these cases indicated that discussions of social control vs. medical responsibility and clear criteria for hospitalization should be incorporated into residency training programs since the emergency room resident faces these tension-producing issues frequently with several different types of patients. Moreover, utilization review criteria may help to set standards which will assist the psychiatrist in making these difficult decisions.", "PMID": 956794}
{"id": "pubmed23n0032_2410", "title": "Phantom limb phenomenon in patients with leprosy.", "content": "The phantom limb phenomenon (PLP) was studied in 42 patients with leprosy who had limb amputation and/or digital shortening; some of the patients also had another type of limb deformity, such as claw-hand or foot-drop, which did not involve significant loss of tissue. Thirty-eight (90 per cent) of the patients reported having at least one phantom limb (PL) for a missing or deformed part. Associated with each of the three types of limb defects, two descriptively and experientially distinct types of PLs were found: the paresthetic or typical (TPL) and the painless or natural (NPL). Descriptions of the TPL and the NPL are given. The findings were compared to various reports, particularly those of Stetter and Frederiks. The leprosy TPL was similar both to the PL of amputees as frequently described in the literature, and to what Stetter and Frederiks termed the PL sensations. A close correspondence, if not identity, was noted between the NPL of leprosy patients and the equivalent categories of Stetter's PL experience and Frederiks' PL as such. Included in these three designations are aspects of the PLP that are associated with the normal, intact limb; these aspects are thought to be usual concomitants of the PLP and to constitute manifestations of the persistence of the normal body image. From our findings, we conclude that for the appearance of a PLP, the actual loss of a part of a limb, whether slow or sudden, is not required, but the loss of its sensorimotor functions is sufficient.", "contents": "Phantom limb phenomenon in patients with leprosy. The phantom limb phenomenon (PLP) was studied in 42 patients with leprosy who had limb amputation and/or digital shortening; some of the patients also had another type of limb deformity, such as claw-hand or foot-drop, which did not involve significant loss of tissue. Thirty-eight (90 per cent) of the patients reported having at least one phantom limb (PL) for a missing or deformed part. Associated with each of the three types of limb defects, two descriptively and experientially distinct types of PLs were found: the paresthetic or typical (TPL) and the painless or natural (NPL). Descriptions of the TPL and the NPL are given. The findings were compared to various reports, particularly those of Stetter and Frederiks. The leprosy TPL was similar both to the PL of amputees as frequently described in the literature, and to what Stetter and Frederiks termed the PL sensations. A close correspondence, if not identity, was noted between the NPL of leprosy patients and the equivalent categories of Stetter's PL experience and Frederiks' PL as such. Included in these three designations are aspects of the PLP that are associated with the normal, intact limb; these aspects are thought to be usual concomitants of the PLP and to constitute manifestations of the persistence of the normal body image. From our findings, we conclude that for the appearance of a PLP, the actual loss of a part of a limb, whether slow or sudden, is not required, but the loss of its sensorimotor functions is sufficient.", "PMID": 956795}
{"id": "pubmed23n0032_2411", "title": "Psychological characteristics of women with sexual inhibition (frigidity) in six clinics.", "content": "Traditional psychiatric thought has equated the inability of a female to obtain an orgasm (particularly a vaginal orgasm during intercourse) with psychological maladjustment. The research literature generally does not support this position. The present paper describes the psychological characteristics of 44 frigid women who applied for treatment of their sexual problem at two university hospital sex clinics and 26 women selected for treatment by the same criterion, but treated in private practice. Presented for comparison are 53 female neurotics from a Temple University Hospital psychotherapy study, a sample of 65 consecutive female walk-ins of mixed psychiatric diagnosis from the Psychiatric Outpatient Clinic of Temple Hospital, and a group of 35 female college student sophomores from Temple University who comprised the normal sample. A battery of psychological tests including the MMPI, the Institute for Personality and Ability Testing--Self-Evaluation Form (IPAT), the Symptom Check List, and the Eysenck Personality Inventory were given to each group. The data indicate that female patients who apply to a sex dysfunction clinic, complaining primarily of sexual inhibition, appear as a group identical to a normal control group in terms of their psychological profile and less neurotic than psychiatric outpatients, with the exception that the normals were less depressed. When women with primary orgasmic dysfunction from the above three samples were combined and compared to those with secondary orgasmic dysfunction (using Masters and Johnson's criterion), the groups were identical, at least from a global psychological perspective. We suggest that little else can be gained by assessing global personality characteristics. Without discarding the primary and secondary classifications a potentially more fruitful approach would be to develop instruments that would measure specific dimensions, such as sexual misinformation, specific sexual anxiety or guilt, or resentment or hostility directed toward the immediate sexual partner. A scale to measure specific phobic-like sexual anxiety, in addition, would have treatment implications because of the recently demonstrated effectiveness of specific anxiety-reducing techniques, such as systematic desensitization. Specific scales can also be useful in the assessment and prediction of outcome in psychotherapy.", "contents": "Psychological characteristics of women with sexual inhibition (frigidity) in six clinics. Traditional psychiatric thought has equated the inability of a female to obtain an orgasm (particularly a vaginal orgasm during intercourse) with psychological maladjustment. The research literature generally does not support this position. The present paper describes the psychological characteristics of 44 frigid women who applied for treatment of their sexual problem at two university hospital sex clinics and 26 women selected for treatment by the same criterion, but treated in private practice. Presented for comparison are 53 female neurotics from a Temple University Hospital psychotherapy study, a sample of 65 consecutive female walk-ins of mixed psychiatric diagnosis from the Psychiatric Outpatient Clinic of Temple Hospital, and a group of 35 female college student sophomores from Temple University who comprised the normal sample. A battery of psychological tests including the MMPI, the Institute for Personality and Ability Testing--Self-Evaluation Form (IPAT), the Symptom Check List, and the Eysenck Personality Inventory were given to each group. The data indicate that female patients who apply to a sex dysfunction clinic, complaining primarily of sexual inhibition, appear as a group identical to a normal control group in terms of their psychological profile and less neurotic than psychiatric outpatients, with the exception that the normals were less depressed. When women with primary orgasmic dysfunction from the above three samples were combined and compared to those with secondary orgasmic dysfunction (using Masters and Johnson's criterion), the groups were identical, at least from a global psychological perspective. We suggest that little else can be gained by assessing global personality characteristics. Without discarding the primary and secondary classifications a potentially more fruitful approach would be to develop instruments that would measure specific dimensions, such as sexual misinformation, specific sexual anxiety or guilt, or resentment or hostility directed toward the immediate sexual partner. A scale to measure specific phobic-like sexual anxiety, in addition, would have treatment implications because of the recently demonstrated effectiveness of specific anxiety-reducing techniques, such as systematic desensitization. Specific scales can also be useful in the assessment and prediction of outcome in psychotherapy.", "PMID": 956796}
{"id": "pubmed23n0032_2412", "title": "Truax factors, speech characteristics, and therapeutic outcome.", "content": "Initial interview ratings of therapist accurate empathy, unconditional positive regard, and self-congruence were correlated with nonlexical speech characteristics and outcome of individual psychotherapy. Speech characteristics of both patients (greater total speech time, shorter pause time following therapists' comments, and faster reaction times) and therapists (fewer speech units) were significantly related to improved outcome. While Rogers-Truax factors were unrelated to outcome, they were related to speech characteristics of both patient and therapist. Furthermore, patients whose therapists showed high levels of the Truax factors tended to increase their average speech durations and total speech time during the course of the initial interview.", "contents": "Truax factors, speech characteristics, and therapeutic outcome. Initial interview ratings of therapist accurate empathy, unconditional positive regard, and self-congruence were correlated with nonlexical speech characteristics and outcome of individual psychotherapy. Speech characteristics of both patients (greater total speech time, shorter pause time following therapists' comments, and faster reaction times) and therapists (fewer speech units) were significantly related to improved outcome. While Rogers-Truax factors were unrelated to outcome, they were related to speech characteristics of both patient and therapist. Furthermore, patients whose therapists showed high levels of the Truax factors tended to increase their average speech durations and total speech time during the course of the initial interview.", "PMID": 956797}
{"id": "pubmed23n0032_2413", "title": "Therapists' physiological activation and patient difficulty.", "content": "The differential effects of an easy and difficult-to-interview patient on therapist self-reported behavioral arousal and physiological activation were observed. Eighteen experienced white male psychiatrists each interviewed two patient simulators believing they were real patients. Each actress was programed to act the part of a difficult-to-interview patient and an easy-to-interview patient. Each randomly played either part as assigned and the order of patient and role was counterbalanced. Differences in patient roles were validated by the participating therapists and independent raters. Fifteen-minute rest periods with soft music preceded each therapy session such that a complete experimental session consisted of rest 1, therapy 1, rest 2, and therapy 2. Self-reported arousal was assessed during each of the four periods by Thayer's factors from the Nowlis Mood Adjective List. Physiological activation was measured by electromyogram, heart rate, blood pressure (systolic and diastolic), and skin conductance. Therapist self-reported behavioral arousal and measured physiological activation during therapy were significantly greater than during rest. The difficulty of the patient did not appear to affect either behavioral or physiological activation level of the therapists. Several explanations for this are explored.", "contents": "Therapists' physiological activation and patient difficulty. The differential effects of an easy and difficult-to-interview patient on therapist self-reported behavioral arousal and physiological activation were observed. Eighteen experienced white male psychiatrists each interviewed two patient simulators believing they were real patients. Each actress was programed to act the part of a difficult-to-interview patient and an easy-to-interview patient. Each randomly played either part as assigned and the order of patient and role was counterbalanced. Differences in patient roles were validated by the participating therapists and independent raters. Fifteen-minute rest periods with soft music preceded each therapy session such that a complete experimental session consisted of rest 1, therapy 1, rest 2, and therapy 2. Self-reported arousal was assessed during each of the four periods by Thayer's factors from the Nowlis Mood Adjective List. Physiological activation was measured by electromyogram, heart rate, blood pressure (systolic and diastolic), and skin conductance. Therapist self-reported behavioral arousal and measured physiological activation during therapy were significantly greater than during rest. The difficulty of the patient did not appear to affect either behavioral or physiological activation level of the therapists. Several explanations for this are explored.", "PMID": 956798}
{"id": "pubmed23n0032_2414", "title": "Short term memory for numerousness in schizophrenic young adults.", "content": "The short term memory of schizophrenics for random dot patterns was examined by a delayed comparison procedure. In experiment 1, the 10 schizophrenics and 10 normals compared a fixed dot pattern (standard) with a variable dot pattern (compairson) appearing 2 or 8 seconds later and decided whether the comparison stimulus had \"more\" or \"less\" dots than the standard. Memory strength, indexed by the d' value of signal-detection theory, showed neither group difference nor decay over time. In experiment 2, the interstimulus interval was filled with an unjudged dot pattern (storage interference), and the standard stimulus followed the variable comparison stimulus (encoding difficulty). The memory strength of 17 schizophrenics and 17 normals was severely impaired, and their memory strength weakened over time, but again, no group differences were found. In both experiments, the two groups showed a strong bias (beta) in underestimating the first (to-be-remembered) stimulus. It was concluded that schizophrenics' short term perceptual memory for nonverbal stimuli remains good.", "contents": "Short term memory for numerousness in schizophrenic young adults. The short term memory of schizophrenics for random dot patterns was examined by a delayed comparison procedure. In experiment 1, the 10 schizophrenics and 10 normals compared a fixed dot pattern (standard) with a variable dot pattern (compairson) appearing 2 or 8 seconds later and decided whether the comparison stimulus had \"more\" or \"less\" dots than the standard. Memory strength, indexed by the d' value of signal-detection theory, showed neither group difference nor decay over time. In experiment 2, the interstimulus interval was filled with an unjudged dot pattern (storage interference), and the standard stimulus followed the variable comparison stimulus (encoding difficulty). The memory strength of 17 schizophrenics and 17 normals was severely impaired, and their memory strength weakened over time, but again, no group differences were found. In both experiments, the two groups showed a strong bias (beta) in underestimating the first (to-be-remembered) stimulus. It was concluded that schizophrenics' short term perceptual memory for nonverbal stimuli remains good.", "PMID": 956799}
{"id": "pubmed23n0032_2415", "title": "Voice predicts affect during psychotherapy.", "content": "This study examined the relationship between emotions and the frequency and power characteristics of the voice in psychotherapy. The intensity of fear, anger, depression, and total affect in each of four interviews with one patient was rated every 20 seconds on a nine-point scale. Significant agreement among judges was achieved. Voice samples from each epoch in which there was sufficient speech were subjected to spectral analysis of the frequencies between 0 and 1000 HZ. These spectra were scored for nine frequency and power parameters. Multiple linear regression equations were then developed from two interviews, using the nine voice spectral variables as predictors and the mean ratings for each affect as the criterion variables. Significant multiple correlations were achieved between every rated affect and various combinations of voice variables. The beta weights and constants from these equations were then employed in the successful prediction of levels of anger, fear, depression, and total affect in one interview, and the levels of depression and total affect in another interview. In addition, epochs of conflict differed from \"pure\" affect epochs, and pure epochs of anger, fear, and depression differed from each other in various frequency and power characteristics of the voice. Voice spectral measures may be an objective means of identifying and quantifying affect in psychotherapy.", "contents": "Voice predicts affect during psychotherapy. This study examined the relationship between emotions and the frequency and power characteristics of the voice in psychotherapy. The intensity of fear, anger, depression, and total affect in each of four interviews with one patient was rated every 20 seconds on a nine-point scale. Significant agreement among judges was achieved. Voice samples from each epoch in which there was sufficient speech were subjected to spectral analysis of the frequencies between 0 and 1000 HZ. These spectra were scored for nine frequency and power parameters. Multiple linear regression equations were then developed from two interviews, using the nine voice spectral variables as predictors and the mean ratings for each affect as the criterion variables. Significant multiple correlations were achieved between every rated affect and various combinations of voice variables. The beta weights and constants from these equations were then employed in the successful prediction of levels of anger, fear, depression, and total affect in one interview, and the levels of depression and total affect in another interview. In addition, epochs of conflict differed from \"pure\" affect epochs, and pure epochs of anger, fear, and depression differed from each other in various frequency and power characteristics of the voice. Voice spectral measures may be an objective means of identifying and quantifying affect in psychotherapy.", "PMID": 956800}
{"id": "pubmed23n0032_2416", "title": "Depressive disorders in Vietnam returnees.", "content": "A random sample of 470 Army enlisted men who served tours of duty in Vietnam and returned to the United States in September, 1971 was selected from military records. Between May and September, 1972, these servicemen were located and personally interviewed. Additional information was obtained from their military records and from Veterans Administration files. Interviews were obtained for 95% and military records for 99%. Veterans Administration records were available for 22% of the sample. Depressive disorders appeared to be a significant problem in these enlistees after their return. Twenty-six per cent of the total sample reported at least some symptoms of depression, and 7 per cent reported a full affective syndrome. A third of those with depressive syndromes have had psychiatric care since their return. The association of depression with combat, use of illicit drugs, and other pre- and post-Vietnam variables is discussed.", "contents": "Depressive disorders in Vietnam returnees. A random sample of 470 Army enlisted men who served tours of duty in Vietnam and returned to the United States in September, 1971 was selected from military records. Between May and September, 1972, these servicemen were located and personally interviewed. Additional information was obtained from their military records and from Veterans Administration files. Interviews were obtained for 95% and military records for 99%. Veterans Administration records were available for 22% of the sample. Depressive disorders appeared to be a significant problem in these enlistees after their return. Twenty-six per cent of the total sample reported at least some symptoms of depression, and 7 per cent reported a full affective syndrome. A third of those with depressive syndromes have had psychiatric care since their return. The association of depression with combat, use of illicit drugs, and other pre- and post-Vietnam variables is discussed.", "PMID": 956801}
{"id": "pubmed23n0032_2417", "title": "Do depressed patients show thought disorder?", "content": "Twenty-one patients suffering from major depressive disorder are compared for evidence of thought disorder on admission and at the time of discharge. Two aspects of cognition are evaluated: the ability to abstract, and the quality of association. These aspects of cognition were tested using the Shipley-Hartford, the Raven Progressive Matrices, and the Goldstein-Scheerer Object Sorting Test. Althouh the patients showed some improvement in ability to abstract, and although their associations became somewhat less underinclusive or improverished, these improvements did not achieve statistical significance. Consequently, this group of depressed patients did not appear to show any thought disorder of the type under investigation.", "contents": "Do depressed patients show thought disorder? Twenty-one patients suffering from major depressive disorder are compared for evidence of thought disorder on admission and at the time of discharge. Two aspects of cognition are evaluated: the ability to abstract, and the quality of association. These aspects of cognition were tested using the Shipley-Hartford, the Raven Progressive Matrices, and the Goldstein-Scheerer Object Sorting Test. Althouh the patients showed some improvement in ability to abstract, and although their associations became somewhat less underinclusive or improverished, these improvements did not achieve statistical significance. Consequently, this group of depressed patients did not appear to show any thought disorder of the type under investigation.", "PMID": 956802}
{"id": "pubmed23n0032_2418", "title": "A comparison of abstractive and nonabstractive deficits in schizophrenics and psychiatric controls.", "content": "Chapman and Chapman (Disordered Thought in Schizophrenia, Appleton-Century-Crofts, New York, 1973) have suggested that findings of abstract thinking deficits in schizophrenia could be functions of control task artifacts. This paper describes two projects in which the abilities of schizophrenics and psychiatric hospital controls who had been closely matched on a wide variety of vocabulary and nonvocabulary control tasks to perform several types of abstract thinking tasks were compared. No differences on the mean scores of the schizophrenic and control patients appeared on four of five measures used. On the fifth, a logical reasoning test, the schizophrenics were inferior to controls. The results suggest that many of the differences reported earlier between schizophrenics and nonschizophrenics on abstraction tasks may result from the heavy reliance of researchers on vocabulary as a control matching test, and its peculiar resistance to deterioration in schizophrenia. The results also suggest that inability to use logic properly may be.", "contents": "A comparison of abstractive and nonabstractive deficits in schizophrenics and psychiatric controls. Chapman and Chapman (Disordered Thought in Schizophrenia, Appleton-Century-Crofts, New York, 1973) have suggested that findings of abstract thinking deficits in schizophrenia could be functions of control task artifacts. This paper describes two projects in which the abilities of schizophrenics and psychiatric hospital controls who had been closely matched on a wide variety of vocabulary and nonvocabulary control tasks to perform several types of abstract thinking tasks were compared. No differences on the mean scores of the schizophrenic and control patients appeared on four of five measures used. On the fifth, a logical reasoning test, the schizophrenics were inferior to controls. The results suggest that many of the differences reported earlier between schizophrenics and nonschizophrenics on abstraction tasks may result from the heavy reliance of researchers on vocabulary as a control matching test, and its peculiar resistance to deterioration in schizophrenia. The results also suggest that inability to use logic properly may be.", "PMID": 956803}
{"id": "pubmed23n0032_2419", "title": "Skin conductance responding/nonresponding and pupiliometrics in chronic schizophrenia. A confirmation of Gruzelier and Venables.", "content": "In a number of reports, Gruzelier and Venables have demonstrated that about 50% of the schizophrenic population do not show the skin conductance orienting response (S.C.O.R.), while those that do respond, do not show normal habituation. Zahn (Orienting response in schizophrenics. J. Nerv. Ment. Dis., 162: 195-199, 1976) has questioned the bimodality of the S.C.O.R. in schizophrenia and suggests that responding/nonresponding could well be due to differential effects of phenothiazine medication. In two experiments (with equally medicated patients), in two different hospitals, the present report confirms the finding of a large proportion of nonresponding in schizophrenia but also suggests that both normal and nonhabituation are seen within schizophrenics and normals. Differential pupillary dilation and constriction parameters are seen in responders and nonresponders although both of these patient groups show tachycardia (the most usual effect of phenothiazines) in comparison to the normal group. The results do not fit easily into the differential effect of phenothiazines hypothesis proposed by Zahn.", "contents": "Skin conductance responding/nonresponding and pupiliometrics in chronic schizophrenia. A confirmation of Gruzelier and Venables. In a number of reports, Gruzelier and Venables have demonstrated that about 50% of the schizophrenic population do not show the skin conductance orienting response (S.C.O.R.), while those that do respond, do not show normal habituation. Zahn (Orienting response in schizophrenics. J. Nerv. Ment. Dis., 162: 195-199, 1976) has questioned the bimodality of the S.C.O.R. in schizophrenia and suggests that responding/nonresponding could well be due to differential effects of phenothiazine medication. In two experiments (with equally medicated patients), in two different hospitals, the present report confirms the finding of a large proportion of nonresponding in schizophrenia but also suggests that both normal and nonhabituation are seen within schizophrenics and normals. Differential pupillary dilation and constriction parameters are seen in responders and nonresponders although both of these patient groups show tachycardia (the most usual effect of phenothiazines) in comparison to the normal group. The results do not fit easily into the differential effect of phenothiazines hypothesis proposed by Zahn.", "PMID": 956804}
{"id": "pubmed23n0032_2420", "title": "Phenothiazine death: an unusual case report.", "content": "This paper is a case report of sudden, unexpected autopsy-negative death in a hyperactive, hopspitalized patient being treated with a modified schedule of rapid tranquilization. The data of the study suggest that death was caused by phenothiazines and that phenothiazines can cause clinically significant depression of the medullary respiratory centers of the brain stem. Clinicians should monitor rate and quality of respirations as well as blood pressure closely when schedules of rapid tranquilization are implemented.", "contents": "Phenothiazine death: an unusual case report. This paper is a case report of sudden, unexpected autopsy-negative death in a hyperactive, hopspitalized patient being treated with a modified schedule of rapid tranquilization. The data of the study suggest that death was caused by phenothiazines and that phenothiazines can cause clinically significant depression of the medullary respiratory centers of the brain stem. Clinicians should monitor rate and quality of respirations as well as blood pressure closely when schedules of rapid tranquilization are implemented.", "PMID": 956805}
{"id": "pubmed23n0032_2421", "title": "Changes in environmental life events and their relationship to psychiatric hospital admissions.", "content": "This study was designed to determine if differences exist in the number of stressful life events experienced by individuals precipitating a first admission vs. readmission to an inpatient psychiatric facility based on diagnostic classification and sex. The subjects consisted of adult patients diagnosed as schizophrenic, depressive neurotic, or chemically addicted, who had not been hospitalized for psychiatric problems for at least 12 months prior to the current hospitalization. The Holmes and Rahe Social Readjustment Rating Scale was used to quantify the life stresses experienced. A 3 X 2 X 2 analysis of variance showed no significant differences between groups.", "contents": "Changes in environmental life events and their relationship to psychiatric hospital admissions. This study was designed to determine if differences exist in the number of stressful life events experienced by individuals precipitating a first admission vs. readmission to an inpatient psychiatric facility based on diagnostic classification and sex. The subjects consisted of adult patients diagnosed as schizophrenic, depressive neurotic, or chemically addicted, who had not been hospitalized for psychiatric problems for at least 12 months prior to the current hospitalization. The Holmes and Rahe Social Readjustment Rating Scale was used to quantify the life stresses experienced. A 3 X 2 X 2 analysis of variance showed no significant differences between groups.", "PMID": 956806}
{"id": "pubmed23n0032_2422", "title": "Patient variables supporting chronic illness. A scale for measuring attitudes toward respiratory illness and hospitalization.", "content": "To enable the study of attitudes as one basis for the maintenance of chronic illness, a scale of patient attitudes toward chronic respiratory illness and hospitalization was developed. Key cluster analysis was performed on a large set of statements which had been rated by patients hospitalized for chronic asthma in a residential treatment setting. The analyses identified six attitudinal clusters: Optimism, Negative Staff Regard, Specific Internal Awareness, External Control, Psychological Stigma, and Authoritarian Attitudes toward Illness and Hospitalization. The clusters showed good internal consistency, reliability, and generality. The low to moderate inter-relationships among clusters suggest the existence of identifiable patterns of attitudes which may be related to differences in coping styles to chronic illness.", "contents": "Patient variables supporting chronic illness. A scale for measuring attitudes toward respiratory illness and hospitalization. To enable the study of attitudes as one basis for the maintenance of chronic illness, a scale of patient attitudes toward chronic respiratory illness and hospitalization was developed. Key cluster analysis was performed on a large set of statements which had been rated by patients hospitalized for chronic asthma in a residential treatment setting. The analyses identified six attitudinal clusters: Optimism, Negative Staff Regard, Specific Internal Awareness, External Control, Psychological Stigma, and Authoritarian Attitudes toward Illness and Hospitalization. The clusters showed good internal consistency, reliability, and generality. The low to moderate inter-relationships among clusters suggest the existence of identifiable patterns of attitudes which may be related to differences in coping styles to chronic illness.", "PMID": 956807}
{"id": "pubmed23n0032_2423", "title": "Distribution of dopamine in the rat cerebral cortex.", "content": "The rat cerebral neocortex was sectioned by 3 transverse cuts and analyzed for dopamine and noradrenaline. Dopamine concentration decreased gradually from the rostral (97 +/- 4 ng/g) to the caudal (59 +/- 2 ng/g) end of the neocortex. Also, the noradrenaline concentration was highest in the rostral part (269 +/- 6 ng/g) and decreased gradually reaching a concentration of 238 +/- 3 ng/g in the caudal part. Since the quotient dopamine/noradrenaline showed similar changes in the neocortex parts investigated the data support the view that dopamine serves as a transmitter in cortical neurons apart from being a precursor for noradrenaline.", "contents": "Distribution of dopamine in the rat cerebral cortex. The rat cerebral neocortex was sectioned by 3 transverse cuts and analyzed for dopamine and noradrenaline. Dopamine concentration decreased gradually from the rostral (97 +/- 4 ng/g) to the caudal (59 +/- 2 ng/g) end of the neocortex. Also, the noradrenaline concentration was highest in the rostral part (269 +/- 6 ng/g) and decreased gradually reaching a concentration of 238 +/- 3 ng/g in the caudal part. Since the quotient dopamine/noradrenaline showed similar changes in the neocortex parts investigated the data support the view that dopamine serves as a transmitter in cortical neurons apart from being a precursor for noradrenaline.", "PMID": 956808}
{"id": "pubmed23n0032_2424", "title": "In vivo assessment of decarboxylase inhibition or potentiation: urinary dopamine and L-dopa output after L-dopa administration.", "content": "In the L-Dopa treated rat, a decreased urinary output of free and conjugated dopamine and an increase in free and conjugated L-Dopa excretion after administration of decarboxylase-inhibiting drugs provide a good in vivo index of Dopa decarboxylase inhibition. With the exception of free dopamine output, which showed an equivocal change, these measurements appear to provide a good yardstick of decarboxylase status in man also. Using this approach, it was not possible to find any evidence of facilitation of decarboxylase action, in L-Dopa-treated parkinsonians given pyridoxine supplements, to account for the ability of this compound to neutralize the beneficial effect of L-Dopa.", "contents": "In vivo assessment of decarboxylase inhibition or potentiation: urinary dopamine and L-dopa output after L-dopa administration. In the L-Dopa treated rat, a decreased urinary output of free and conjugated dopamine and an increase in free and conjugated L-Dopa excretion after administration of decarboxylase-inhibiting drugs provide a good in vivo index of Dopa decarboxylase inhibition. With the exception of free dopamine output, which showed an equivocal change, these measurements appear to provide a good yardstick of decarboxylase status in man also. Using this approach, it was not possible to find any evidence of facilitation of decarboxylase action, in L-Dopa-treated parkinsonians given pyridoxine supplements, to account for the ability of this compound to neutralize the beneficial effect of L-Dopa.", "PMID": 956809}
{"id": "pubmed23n0032_2425", "title": "Separation of pineal extracts by gelfiltration. V. Location by paper chromatography of a sheep pineal principle inhibiting hypophyseal gonadotropic activity.", "content": "Previously we were able to isolate by simple and mild methods from an aqueous sheep pineal extract an inhibiting principle action on the anterior hypophysis of male rats in vitro. This substance could be located by paper electrophoresis. In the present paper we describe the further purification of this active principle by paper chromatography in two different solvents. In two regions possessing a special fluorescence an inhibiting factor of the gonadotropic activity of the anterior pituitary was found in vitro.", "contents": "Separation of pineal extracts by gelfiltration. V. Location by paper chromatography of a sheep pineal principle inhibiting hypophyseal gonadotropic activity. Previously we were able to isolate by simple and mild methods from an aqueous sheep pineal extract an inhibiting principle action on the anterior hypophysis of male rats in vitro. This substance could be located by paper electrophoresis. In the present paper we describe the further purification of this active principle by paper chromatography in two different solvents. In two regions possessing a special fluorescence an inhibiting factor of the gonadotropic activity of the anterior pituitary was found in vitro.", "PMID": 956810}
{"id": "pubmed23n0032_2426", "title": "Study of deterioration in long-term treatment of parkinsonism with L-dopa plus decarboxylase inhibitor.", "content": "35 parkinsonian patients have been treated over 3 years with L-Dopa combined with benserazide. After an impressive improvement during the first months of treatment a slow but significant deterioration of the patient's condition was observed. At the end of the observation period however their condition was still significantly better than before starting the treatment. A reduced mean L-Dopa dosage was ruled out as the cause of this deterioration. Withdrawal of the L-Dopa therapy for a few days in 13 patients provided strong evidence that it is due to the progression of the disease and to a partial loss of L-Dopa efficacy.", "contents": "Study of deterioration in long-term treatment of parkinsonism with L-dopa plus decarboxylase inhibitor. 35 parkinsonian patients have been treated over 3 years with L-Dopa combined with benserazide. After an impressive improvement during the first months of treatment a slow but significant deterioration of the patient's condition was observed. At the end of the observation period however their condition was still significantly better than before starting the treatment. A reduced mean L-Dopa dosage was ruled out as the cause of this deterioration. Withdrawal of the L-Dopa therapy for a few days in 13 patients provided strong evidence that it is due to the progression of the disease and to a partial loss of L-Dopa efficacy.", "PMID": 956811}
{"id": "pubmed23n0032_2427", "title": "Present morality in parkinson's disease: the ratio of observed to expected deaths with a method to calculate expected deaths.", "content": "Excess mortality, i.e. more deaths than expected in a similar normal population, has been reduced in Parkinson's disease by levodopa. A California series and a large collaborative study throughout the United States show the ratio of observed to expected deaths to be normal. In contrast, a New York study was similar patients and a Montreal group of much more severely disabled patients showed greater mortality. The reasons for these differences are unclear, but some variations in results may be attributed to different methods of calculating expected deaths to derive the ratio of observed to expected deaths.", "contents": "Present morality in parkinson's disease: the ratio of observed to expected deaths with a method to calculate expected deaths. Excess mortality, i.e. more deaths than expected in a similar normal population, has been reduced in Parkinson's disease by levodopa. A California series and a large collaborative study throughout the United States show the ratio of observed to expected deaths to be normal. In contrast, a New York study was similar patients and a Montreal group of much more severely disabled patients showed greater mortality. The reasons for these differences are unclear, but some variations in results may be attributed to different methods of calculating expected deaths to derive the ratio of observed to expected deaths.", "PMID": 956812}
{"id": "pubmed23n0032_2428", "title": "Influence of age and time interval between death and autopsy on dopamine and 3-methoxytyramine levels in human basal ganglia.", "content": "The caudate nuclei and putamens of 30 human brains were analyzed for dopamine and its metabolite 3-methoxytyramine, most of which was presumably formed from dopamine postmortally. It was found that dopamine and the sum (dopamine + methoxytyramine) but not methoxytyramine declined with the time interval between death and autopsy as well as with age.", "contents": "Influence of age and time interval between death and autopsy on dopamine and 3-methoxytyramine levels in human basal ganglia. The caudate nuclei and putamens of 30 human brains were analyzed for dopamine and its metabolite 3-methoxytyramine, most of which was presumably formed from dopamine postmortally. It was found that dopamine and the sum (dopamine + methoxytyramine) but not methoxytyramine declined with the time interval between death and autopsy as well as with age.", "PMID": 956813}
{"id": "pubmed23n0032_2429", "title": "Time course of nigrostriatal degeneration in parkinson's disease. A detailed study of influential factors in human brain amine analysis.", "content": "It could be shown that the post mortem analysis of biogenic amines, precursors and metabolites in the human brain are influenced by various parameters. 1. The patient's medical history; long term observations of the course of the disease; age; sex. 2. Terminal illness; duration of terminal illness. 3. Previous treatment with drugs; last drugs. 4. Time interval between last drug treatment and death; time of day and date of last drug consumption. 5. Rapidity of death; time of death; duration of coma. 6. Changes occurring in tissues before death; patients' constitution during terminal illness. 7. Changes in concentration of the biogenic amines, precursors, and metabolites depending on the patient's age. 8. Time between death and necropsy. 9 Dissection of specimen. 10. Period of storage; temperature of storage. 11. Chronbiological rhythm of substances. 12. Methods of assayL 13. Homogeneity of all mentioned parameters in the control group and patient's group. For the first time it could be demonstrated that the time course of nigrostriatal degeneration, independent of the age of the parkinsonian at the beginning of the illness, is linear for the last stage and the denervation progressively increases as the duration of illness progresses.", "contents": "Time course of nigrostriatal degeneration in parkinson's disease. A detailed study of influential factors in human brain amine analysis. It could be shown that the post mortem analysis of biogenic amines, precursors and metabolites in the human brain are influenced by various parameters. 1. The patient's medical history; long term observations of the course of the disease; age; sex. 2. Terminal illness; duration of terminal illness. 3. Previous treatment with drugs; last drugs. 4. Time interval between last drug treatment and death; time of day and date of last drug consumption. 5. Rapidity of death; time of death; duration of coma. 6. Changes occurring in tissues before death; patients' constitution during terminal illness. 7. Changes in concentration of the biogenic amines, precursors, and metabolites depending on the patient's age. 8. Time between death and necropsy. 9 Dissection of specimen. 10. Period of storage; temperature of storage. 11. Chronbiological rhythm of substances. 12. Methods of assayL 13. Homogeneity of all mentioned parameters in the control group and patient's group. For the first time it could be demonstrated that the time course of nigrostriatal degeneration, independent of the age of the parkinsonian at the beginning of the illness, is linear for the last stage and the denervation progressively increases as the duration of illness progresses.", "PMID": 956814}
{"id": "pubmed23n0032_2430", "title": "Synaptic development in the crayfish opener muscle.", "content": "Nerve terminal regions in walking leg opener muscles of several crayfish of different ages (0 to 245 days after hatching) were examined by means of electron microscopy. This muscle is innervated by two axons (excitatory and inhibitory) and at maturity contains three classes of synapse: excitatory and inhibitory neuromuscular synapses, and inhibitory axo-axonal synapses. The muscle itself is initially a syncytium, which gradually becomes subdivided into distinct \"muscle fibers\" as the animal matures. Innervation was not found in the opener muscle just before or just after hatching, but was present in restricted locations on the inner side of the muscle within a few days of hatching. As the muscle enlarged and became subdivided, innervation appeared in various other locations. Synaptic contacts were located in young stages soon after hatching, and in later stages. Morphological differences characteristic of excitatory nerve terminals could be found even at the earliest stages of innervation. Both excitatory and inhibitory synapses, but particularly the former, showed evidence of progressive enlargement to a final size within the first two months, and no evidence for further enlargement of existing synapses thereafter. Synaptic maturation also involved the appearance of presynaptic \"dense bodies\" though to be regions at which transmitter substance is preferentially released. Nerve terminals at different levels of maturation were observed in opener muscles of young crayfish. Clear evidence for differential maturation of the three types of synapse present in this muscle was obtained. The inhibitory neuromuscular synapses attained their final average size and developed their dense bodies sooner than the excitatory neuromuscular synapses. The inhibitory axo-axonal synapses were the last to appear and to mature.", "contents": "Synaptic development in the crayfish opener muscle. Nerve terminal regions in walking leg opener muscles of several crayfish of different ages (0 to 245 days after hatching) were examined by means of electron microscopy. This muscle is innervated by two axons (excitatory and inhibitory) and at maturity contains three classes of synapse: excitatory and inhibitory neuromuscular synapses, and inhibitory axo-axonal synapses. The muscle itself is initially a syncytium, which gradually becomes subdivided into distinct \"muscle fibers\" as the animal matures. Innervation was not found in the opener muscle just before or just after hatching, but was present in restricted locations on the inner side of the muscle within a few days of hatching. As the muscle enlarged and became subdivided, innervation appeared in various other locations. Synaptic contacts were located in young stages soon after hatching, and in later stages. Morphological differences characteristic of excitatory nerve terminals could be found even at the earliest stages of innervation. Both excitatory and inhibitory synapses, but particularly the former, showed evidence of progressive enlargement to a final size within the first two months, and no evidence for further enlargement of existing synapses thereafter. Synaptic maturation also involved the appearance of presynaptic \"dense bodies\" though to be regions at which transmitter substance is preferentially released. Nerve terminals at different levels of maturation were observed in opener muscles of young crayfish. Clear evidence for differential maturation of the three types of synapse present in this muscle was obtained. The inhibitory neuromuscular synapses attained their final average size and developed their dense bodies sooner than the excitatory neuromuscular synapses. The inhibitory axo-axonal synapses were the last to appear and to mature.", "PMID": 956815}
{"id": "pubmed23n0032_2431", "title": "A regional study of developing rat brain: the accumulation and distribution of proteolipid proteins.", "content": "The accumulation and distribution of proteolipid proteins in rat brain and selected brain regions (cerebellum, cerebral cortex, basal ganglia, and hippocampus) were studied during early postnatal development. In whole brain an eightfold increase of proteolipid was observed between ten and 33 days after birth. This was reflected in the separate regions examined where the proteolipid protein content increased six- to ten-fold during the same period. The basal ganglia and cerebral cortex contributed the greatest amount to the total proteolipid present. However, at 28-33 days the greatest concentration (mg/g tissue) was observed in the basal ganglia and hippocampus. When the proteolipid protein preparations were examined by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, distinctive, heterogeneous patterns for each brain region were obtained. Proteolipid from basal ganglia (the region richest in white matter) consisted primarily of two major protein bands with apparent molecular weights of approximately 21,500 and 26,000. Both of these bands dramatically increased in quantity during myelination, and the larger protein coelectrophoresed with isolated myelin proteolipid protein. Both bands were also found present in proteolipid preparations from the other brain regions but in varying amounts relative to the total. The data suggest that the increase in proteolipid observed during this developmental period was due in large measure to the accumulation of myelin-specific proteolipids, but also that a significant proportion of the increase was due to the accumulation of nonmyelin components.", "contents": "A regional study of developing rat brain: the accumulation and distribution of proteolipid proteins. The accumulation and distribution of proteolipid proteins in rat brain and selected brain regions (cerebellum, cerebral cortex, basal ganglia, and hippocampus) were studied during early postnatal development. In whole brain an eightfold increase of proteolipid was observed between ten and 33 days after birth. This was reflected in the separate regions examined where the proteolipid protein content increased six- to ten-fold during the same period. The basal ganglia and cerebral cortex contributed the greatest amount to the total proteolipid present. However, at 28-33 days the greatest concentration (mg/g tissue) was observed in the basal ganglia and hippocampus. When the proteolipid protein preparations were examined by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, distinctive, heterogeneous patterns for each brain region were obtained. Proteolipid from basal ganglia (the region richest in white matter) consisted primarily of two major protein bands with apparent molecular weights of approximately 21,500 and 26,000. Both of these bands dramatically increased in quantity during myelination, and the larger protein coelectrophoresed with isolated myelin proteolipid protein. Both bands were also found present in proteolipid preparations from the other brain regions but in varying amounts relative to the total. The data suggest that the increase in proteolipid observed during this developmental period was due in large measure to the accumulation of myelin-specific proteolipids, but also that a significant proportion of the increase was due to the accumulation of nonmyelin components.", "PMID": 956816}
{"id": "pubmed23n0032_2432", "title": "A mutant of Paramecium with increased relative resting potassium permeability.", "content": "Fast-2, a membrane mutant of Paramecium aurelia, is due to a single-gene mutation and has behavioral abnormalities. Intracellular recordings through changes of external solutions were made. The mutant membrane hyperpolarized when it encountered solutions with low K+ concentration. This hyperpolarization and other associated activities were best observed in Ca- or Na-solutions devoid of K+. Membrane potential was plotted against the concentration of K+ (0.5 to 16 mM) in solutions of fixed Na+ or Ca++ concentration. The slopes of the curves for the mutant membrane were steeper than those for the wild type at the lower concentrations of K+. Inclusion of 2 mM tetraethylammonium chloride (TEA-Cl) counteracted the mutational effects. Spontaneous action potentials in Ba-solution and the electrically evoked action potentials in various solutions are normal in this mutant. We conclude that the resting permeability to K+ relative to the permeabilities to Na+ and Ca++ has been increased by the mutation.", "contents": "A mutant of Paramecium with increased relative resting potassium permeability. Fast-2, a membrane mutant of Paramecium aurelia, is due to a single-gene mutation and has behavioral abnormalities. Intracellular recordings through changes of external solutions were made. The mutant membrane hyperpolarized when it encountered solutions with low K+ concentration. This hyperpolarization and other associated activities were best observed in Ca- or Na-solutions devoid of K+. Membrane potential was plotted against the concentration of K+ (0.5 to 16 mM) in solutions of fixed Na+ or Ca++ concentration. The slopes of the curves for the mutant membrane were steeper than those for the wild type at the lower concentrations of K+. Inclusion of 2 mM tetraethylammonium chloride (TEA-Cl) counteracted the mutational effects. Spontaneous action potentials in Ba-solution and the electrically evoked action potentials in various solutions are normal in this mutant. We conclude that the resting permeability to K+ relative to the permeabilities to Na+ and Ca++ has been increased by the mutation.", "PMID": 956817}
{"id": "pubmed23n0032_2433", "title": "Discharge pattern of single motor units in the tonic vibration reflex of human triceps surae.", "content": "Using a single fibre EMG electrode the firing pattern of 46 motor units in the triceps surae has been studied during vibration of the Achilles tendon at frequencies of 25--200 Hz. Potentials activated in the tonic vibration reflex (TVR) were phase-locked to the vibration cycle but tended to become somewhat less so with continued vibration. The firing pattern of voluntarily activated motor units became locked to the waveform by the application of the vibrator. The discharges of 21 motor units were studied during low threshold (sub-M wave) tetanic stimulation of the tibial nerve at 25--100 Hz. No evidence was found of synchronization of potentials activated in the resulting tonic contraction. During weak voluntary contractions, stimulation also failed to regularize voluntarily activated motor units. The findings can be reconciled by postulating that, in normal man, vibration activates monosynaptic and polysynaptic pathways, the latter circuit being adequate to generate reflex contraction, while the former merely affects the temporal patterning of the motor outflow.", "contents": "Discharge pattern of single motor units in the tonic vibration reflex of human triceps surae. Using a single fibre EMG electrode the firing pattern of 46 motor units in the triceps surae has been studied during vibration of the Achilles tendon at frequencies of 25--200 Hz. Potentials activated in the tonic vibration reflex (TVR) were phase-locked to the vibration cycle but tended to become somewhat less so with continued vibration. The firing pattern of voluntarily activated motor units became locked to the waveform by the application of the vibrator. The discharges of 21 motor units were studied during low threshold (sub-M wave) tetanic stimulation of the tibial nerve at 25--100 Hz. No evidence was found of synchronization of potentials activated in the resulting tonic contraction. During weak voluntary contractions, stimulation also failed to regularize voluntarily activated motor units. The findings can be reconciled by postulating that, in normal man, vibration activates monosynaptic and polysynaptic pathways, the latter circuit being adequate to generate reflex contraction, while the former merely affects the temporal patterning of the motor outflow.", "PMID": 956859}
{"id": "pubmed23n0032_2434", "title": "Diagnostic yield of analysis of the pattern of electrical activity and of individual motor unit potentials in myopathy.", "content": "The analysis of the pattern of electrical activity and of individual motor unit potentials in the same muscle both identified about 90% of 41 patients as having a myopathy. The pattern of electrical activity was analysed during a force which was a fixed fraction of maximum; individual motor unit potentials were analysed during weak effort. The two methods supplement each other as some of the patients were identified only by one or by the other of the two procedures. The parameter of the pattern of electrical activity which was most often abnormal was the ratio: numbers of turns to mean amplitude between turns.", "contents": "Diagnostic yield of analysis of the pattern of electrical activity and of individual motor unit potentials in myopathy. The analysis of the pattern of electrical activity and of individual motor unit potentials in the same muscle both identified about 90% of 41 patients as having a myopathy. The pattern of electrical activity was analysed during a force which was a fixed fraction of maximum; individual motor unit potentials were analysed during weak effort. The two methods supplement each other as some of the patients were identified only by one or by the other of the two procedures. The parameter of the pattern of electrical activity which was most often abnormal was the ratio: numbers of turns to mean amplitude between turns.", "PMID": 956860}
{"id": "pubmed23n0032_2435", "title": "Correlation between diffuse EEG abnormalities and cerebral atrophy in senile dementia.", "content": "Thirty-five elderly patients were investigated because of clinical signs of dementia. The presence or diffuse cerebral atrophy, and its severity, were determined by the use of computed tomography (CT scan). All of the patients were also examined by electroencephalography (EEG), and the presence of diffuse abnormalities, especially diffuse slowing, was noted. Specifically, patients with normal or near-normal EEGs were compared with those with severe diffuse slowing. No correlation between the presence or severity of diffuse EEG abnormalities and the degree of cerebral atrophy as measured by CT scan was found. Though the EEG is clearly identifying physiological dysfunction of nerve cells in demented patients it does not appear to be reliable tool for the prediction of diffuse cerebral atrophy in this population.", "contents": "Correlation between diffuse EEG abnormalities and cerebral atrophy in senile dementia. Thirty-five elderly patients were investigated because of clinical signs of dementia. The presence or diffuse cerebral atrophy, and its severity, were determined by the use of computed tomography (CT scan). All of the patients were also examined by electroencephalography (EEG), and the presence of diffuse abnormalities, especially diffuse slowing, was noted. Specifically, patients with normal or near-normal EEGs were compared with those with severe diffuse slowing. No correlation between the presence or severity of diffuse EEG abnormalities and the degree of cerebral atrophy as measured by CT scan was found. Though the EEG is clearly identifying physiological dysfunction of nerve cells in demented patients it does not appear to be reliable tool for the prediction of diffuse cerebral atrophy in this population.", "PMID": 956861}
{"id": "pubmed23n0032_2436", "title": "Evidence for the presence of a vasoactive substance (possibly involved in the aetiology of cerebral arterial spasm) in cerebrospinal fluid from patients with subarachnoid haemorrhage.", "content": "Cerebrospinal fluid from patients with subarachnoid haemorrhage and cerebral arterial spasm contracted the isolated basilar artery and potentiated contractions produced by 5-hydroxytryptamine, noradrenaline, and six prostaglandins. The unidentified substance in cerebrospinal fluid probably plays a role in the aetiology of cerebral arterial spasm.", "contents": "Evidence for the presence of a vasoactive substance (possibly involved in the aetiology of cerebral arterial spasm) in cerebrospinal fluid from patients with subarachnoid haemorrhage. Cerebrospinal fluid from patients with subarachnoid haemorrhage and cerebral arterial spasm contracted the isolated basilar artery and potentiated contractions produced by 5-hydroxytryptamine, noradrenaline, and six prostaglandins. The unidentified substance in cerebrospinal fluid probably plays a role in the aetiology of cerebral arterial spasm.", "PMID": 956862}
{"id": "pubmed23n0032_2437", "title": "Relationship between plasma and lymphocyte linoleate in multiple sclerosis.", "content": "The linoleate level in total lipids was measured in lymphocytes from control subjects and patients with multiple sclerosis. A small but significant decrease was found in cases of multiple sclerosis. The percentage composition of lymphocyte fatty acids was determined in rats fed diets with various linoleate contents. There was a correlation between lymphocyte linoleate and plasma linoleate in both humans and rats.", "contents": "Relationship between plasma and lymphocyte linoleate in multiple sclerosis. The linoleate level in total lipids was measured in lymphocytes from control subjects and patients with multiple sclerosis. A small but significant decrease was found in cases of multiple sclerosis. The percentage composition of lymphocyte fatty acids was determined in rats fed diets with various linoleate contents. There was a correlation between lymphocyte linoleate and plasma linoleate in both humans and rats.", "PMID": 956863}
{"id": "pubmed23n0032_2438", "title": "Orbicularis oculi reflex in brain death.", "content": "The early and late components of the orbicularis oculi reflex were absent bilaterally in three patients who fulfilled the clinical and electroencephalographic criteria of brain death. A response to peripheral facial nerve stimulation was obtained in all. These preliminary observations suggest that the study of the orbicularis oculi reflex is a simple, objective, bedside neurophysiological test of brain stem function, complementing the electroencephalogram in the diagnosis of brain death.", "contents": "Orbicularis oculi reflex in brain death. The early and late components of the orbicularis oculi reflex were absent bilaterally in three patients who fulfilled the clinical and electroencephalographic criteria of brain death. A response to peripheral facial nerve stimulation was obtained in all. These preliminary observations suggest that the study of the orbicularis oculi reflex is a simple, objective, bedside neurophysiological test of brain stem function, complementing the electroencephalogram in the diagnosis of brain death.", "PMID": 956864}
{"id": "pubmed23n0032_2439", "title": "The Munich Coma Scle (MCS).", "content": "The level of coma can be measured by the assessment of changes of behaviour after different intensities of stimulation. Two additive scales (Guttman scales) for susceptibility to stimulation and reactivity were developed, constituting the Munich Coma Scale (MCS). The findings of previous investigations were repeated in a new sample of 67 comatose patients. Implications of the additivity of reactivity are discussed and related to the concept of 'behavioural arousal' advanced in the recent literature.", "contents": "The Munich Coma Scle (MCS). The level of coma can be measured by the assessment of changes of behaviour after different intensities of stimulation. Two additive scales (Guttman scales) for susceptibility to stimulation and reactivity were developed, constituting the Munich Coma Scale (MCS). The findings of previous investigations were repeated in a new sample of 67 comatose patients. Implications of the additivity of reactivity are discussed and related to the concept of 'behavioural arousal' advanced in the recent literature.", "PMID": 956865}
{"id": "pubmed23n0032_2440", "title": "Alexander's disease presenting as astrocytoma.", "content": "A case of progressive fibrinoid degeneration of astrocytes is described. Symptoms began with convulsions at the age of 6 weeks and cerebral tumour was suspected because of enlargement of the head, increased intracranial tension, and marked proliferation of astrocytes in the brain biopsy which was interpreted as an astrocytoma. Fibres and granules staining deeply with PTAH are a constant feature of the few cases so far described. The aetiology is unknown but an inherited metabolic defect of astrocytes may be responsible.", "contents": "Alexander's disease presenting as astrocytoma. A case of progressive fibrinoid degeneration of astrocytes is described. Symptoms began with convulsions at the age of 6 weeks and cerebral tumour was suspected because of enlargement of the head, increased intracranial tension, and marked proliferation of astrocytes in the brain biopsy which was interpreted as an astrocytoma. Fibres and granules staining deeply with PTAH are a constant feature of the few cases so far described. The aetiology is unknown but an inherited metabolic defect of astrocytes may be responsible.", "PMID": 956866}
{"id": "pubmed23n0032_2441", "title": "The shoulder girdle disc.", "content": "Weakness and wasting of shoulder girdle muscles occurred in 40 cases of cervical spondylosis at the level C 3/4 intervertebral space with spinal cord, and lower with nerve root compression. Often there was no sensory loss. Prompt recovery followed Cloward's operation in more than half the cases. The pathogenesis and the need for early diagnosis and radical treatment of the condition are considered.", "contents": "The shoulder girdle disc. Weakness and wasting of shoulder girdle muscles occurred in 40 cases of cervical spondylosis at the level C 3/4 intervertebral space with spinal cord, and lower with nerve root compression. Often there was no sensory loss. Prompt recovery followed Cloward's operation in more than half the cases. The pathogenesis and the need for early diagnosis and radical treatment of the condition are considered.", "PMID": 956868}
{"id": "pubmed23n0032_2442", "title": "Hypertrophy of the branchial muscles. A case with unusual features.", "content": "A detailed morphological and histochemical description of pathological changes previously unreported in hypertrophy of the masseter muscle is presented. The patient differed from earlier cases in reacting adversely to anaesthetic agents.", "contents": "Hypertrophy of the branchial muscles. A case with unusual features. A detailed morphological and histochemical description of pathological changes previously unreported in hypertrophy of the masseter muscle is presented. The patient differed from earlier cases in reacting adversely to anaesthetic agents.", "PMID": 956867}
{"id": "pubmed23n0032_2443", "title": "Lateral canthal advancement of the supraorbital margin. A new corrective technique in the treatment of coronal synostosis.", "content": "In coronal synostosis, in addition to fusion of the coronal suture, the frontosphenoidal and frontoethmoidal sutures are usually closed. A linear craniectomy along the coronal sutures does not affect the synostotic process at the base of the skull. The facility with which the supraorbital margin could be mobilized in Tessier's method of craniofacial repair suggested to us that we could easily modify our approach to coronal synostosis and advance the supraorbital margin, creating an artificial suture at the base of the skull and allowing for proper correction of this disorder. During the past 3 years, we have treated 15 patients with coronal synostosis by this technique, which we have termed lateral canthal advancement. The method of this form of surgical management and its results are discussed.", "contents": "Lateral canthal advancement of the supraorbital margin. A new corrective technique in the treatment of coronal synostosis. In coronal synostosis, in addition to fusion of the coronal suture, the frontosphenoidal and frontoethmoidal sutures are usually closed. A linear craniectomy along the coronal sutures does not affect the synostotic process at the base of the skull. The facility with which the supraorbital margin could be mobilized in Tessier's method of craniofacial repair suggested to us that we could easily modify our approach to coronal synostosis and advance the supraorbital margin, creating an artificial suture at the base of the skull and allowing for proper correction of this disorder. During the past 3 years, we have treated 15 patients with coronal synostosis by this technique, which we have termed lateral canthal advancement. The method of this form of surgical management and its results are discussed.", "PMID": 956873}
{"id": "pubmed23n0032_2444", "title": "Physiopathogenesis of subdural hematomas. Part 1: Histological and biochemical comparisons of subcutaneous hematoma in rats with subdural hematoma in man.", "content": "The dorsal subcutaneous injection in rats of 12 ml or more of autologous hemolyzed whole blood clotted in situ induced the formation of sterile, hemispheric lesions, 47% of which showed an initial decrease in volume followed by a progressive enlargement. The behavior, histology, and biochemical characteristics of the liquid contents of these lesions were found to be remarkably similar to those of subdural hematomas in man. To evaluate the role of the various blood components that may have influenced the formation and growth of these lesions, more than 150 clots composed of human platelet-free plasma, autologous hemolyzed blood, or autologous whole blood were implanted subcutaneously in rats by either surgical or injection techniques. The in vitro behavior of the different clots used was also assessed. This systematic approach led to the following conclusions: 1) the composition and volumes of the clots are critical variables; 2) plasma-fibrin provides the matrix shape of the lesions; 3) the breakdown products derived from erythrocytes, hemoglobin, leukocytes, and other solid blood elements induce neomembrane formation and contribute to the lesion's subsequent growth; and 4) inflammation mechanisms appear to be essential, while cerebrospinal fluid plays no discernable role in this process.", "contents": "Physiopathogenesis of subdural hematomas. Part 1: Histological and biochemical comparisons of subcutaneous hematoma in rats with subdural hematoma in man. The dorsal subcutaneous injection in rats of 12 ml or more of autologous hemolyzed whole blood clotted in situ induced the formation of sterile, hemispheric lesions, 47% of which showed an initial decrease in volume followed by a progressive enlargement. The behavior, histology, and biochemical characteristics of the liquid contents of these lesions were found to be remarkably similar to those of subdural hematomas in man. To evaluate the role of the various blood components that may have influenced the formation and growth of these lesions, more than 150 clots composed of human platelet-free plasma, autologous hemolyzed blood, or autologous whole blood were implanted subcutaneously in rats by either surgical or injection techniques. The in vitro behavior of the different clots used was also assessed. This systematic approach led to the following conclusions: 1) the composition and volumes of the clots are critical variables; 2) plasma-fibrin provides the matrix shape of the lesions; 3) the breakdown products derived from erythrocytes, hemoglobin, leukocytes, and other solid blood elements induce neomembrane formation and contribute to the lesion's subsequent growth; and 4) inflammation mechanisms appear to be essential, while cerebrospinal fluid plays no discernable role in this process.", "PMID": 956874}
{"id": "pubmed23n0032_2445", "title": "Physiopathogenesis of subdural hematomas. Part 2: Inhibition of growth of experimental hematomas with dexamethasone.", "content": "A previously described experimental hematoma model, achieved by the subcutaneous injection of 12 ml of autologous hemolyzed blood clotted in situ, was made in 33 rats. Sventeen animals served as controls; the other 16 received daily intra-muscular injections of dexamethasone. After an initial decrease in size, 47% of the lesions in the control animals enlarged to a mean weight of 12.1 +/- 2.5 gm, while the lesions in the 16 steroid-treated rats weighted 3.2 +/- 0.70 gm (p less than 0.01). Histologically, lesions from the steroid group showed absence of neomembrane formation. These data offer further support to the theory that the neomembrane development and subsequent enlargement of subdural hematomas is due to inflammatory reactions of tissues in contact with large blood clots.", "contents": "Physiopathogenesis of subdural hematomas. Part 2: Inhibition of growth of experimental hematomas with dexamethasone. A previously described experimental hematoma model, achieved by the subcutaneous injection of 12 ml of autologous hemolyzed blood clotted in situ, was made in 33 rats. Sventeen animals served as controls; the other 16 received daily intra-muscular injections of dexamethasone. After an initial decrease in size, 47% of the lesions in the control animals enlarged to a mean weight of 12.1 +/- 2.5 gm, while the lesions in the 16 steroid-treated rats weighted 3.2 +/- 0.70 gm (p less than 0.01). Histologically, lesions from the steroid group showed absence of neomembrane formation. These data offer further support to the theory that the neomembrane development and subsequent enlargement of subdural hematomas is due to inflammatory reactions of tissues in contact with large blood clots.", "PMID": 956875}
{"id": "pubmed23n0032_2446", "title": "Electron microscopic study on the satellite cell in the muscle of Duchenne muscular dystrophy.", "content": "The ultrastructure and the distribution frequency of the satellite cells of biopsied muscle fibers from 10 patients with Duchenne muscular dystrophy (PMD-D) and 10 patients free of neuromuscular diseases were studied. The increase in the number of satellite cells in muscles of patients with PMD-D over those in the normal control cases were noted. Markedly increased pinocytotic vesicles or the thin and thick filaments were found in these satellite cells. The possible role of satellite cells was discussed.", "contents": "Electron microscopic study on the satellite cell in the muscle of Duchenne muscular dystrophy. The ultrastructure and the distribution frequency of the satellite cells of biopsied muscle fibers from 10 patients with Duchenne muscular dystrophy (PMD-D) and 10 patients free of neuromuscular diseases were studied. The increase in the number of satellite cells in muscles of patients with PMD-D over those in the normal control cases were noted. Markedly increased pinocytotic vesicles or the thin and thick filaments were found in these satellite cells. The possible role of satellite cells was discussed.", "PMID": 956871}
{"id": "pubmed23n0032_2447", "title": "Clinical and pathological study of 24 cases of gliosarcoma.", "content": "The authors review the clinical and pathological features of 24 patients with gliosarcoma. The study revealed the following findings. Gliosarcoma occurs more frequently than is indicated in the literature, and in our series was present in 8% of all cases of glioblastoma multiforme. The presenting features are not significantly different from those of glioblastoma multiforme. The lesion often presents itself at surgery as a firm, well circumscribed mass within the temporal lobe, and at surgery it is commonly mistaken for a meningioma. There is an increased likelihood of metastasis compared to that of glioblastoma. The prognosis is no worse than that of glioblastoma, in spite of the addition of sarcomatous elements.", "contents": "Clinical and pathological study of 24 cases of gliosarcoma. The authors review the clinical and pathological features of 24 patients with gliosarcoma. The study revealed the following findings. Gliosarcoma occurs more frequently than is indicated in the literature, and in our series was present in 8% of all cases of glioblastoma multiforme. The presenting features are not significantly different from those of glioblastoma multiforme. The lesion often presents itself at surgery as a firm, well circumscribed mass within the temporal lobe, and at surgery it is commonly mistaken for a meningioma. There is an increased likelihood of metastasis compared to that of glioblastoma. The prognosis is no worse than that of glioblastoma, in spite of the addition of sarcomatous elements.", "PMID": 956876}
{"id": "pubmed23n0032_2448", "title": "Experimental autoimmune myasthenia gravis: a sequential and quantitative study of the neuromuscular junction ultrastructure and electrophysiologic correlations.", "content": "Neuromuscular junction ultrastructure in rat forelimb digit extensor muscle was sequentially and quantitatively investigated in experimental autoimmune myasthenia gravis (EAMG). Experimental animals were immunized with highly purified eel electroplax acetylcholine receptor protein plus complete Freund's adjuvant and B pertussis vaccine; control animals received only adjuvant and vaccine. During the first 7 days (latent period) after immunization end-plate structure and neuromuscular transmission remained normal in the experimental group. Between day 7 and 11 (acute phase) mononuclear cells infiltrated those regions of muscle where the end-plates were located and there was intense degeneration of the postsynaptic regions with splitting away of abnormal junctional folds from the underlying muscle fibers. Macrophages entered the gaps thus formed and removed the degenerating folds by phagocytosis. The nerve terminals were displaced from their usual location but maintained their structural integrity. Neuromuscular transmission was blocked in many muscle fibers. Miniature end-plate potentias (MEPPs), detectable in only a few fibers, were of abnormally low amplitude. After day 11 (chronic phase) the nerve terminals returned to the highly simplified postsynaptic folds became reconstituted and again degenerated. Immature junctions with poorly differentiated postsynaptic regions and nerve sprouts near end-plates were also observed. In two animals relapsing during the chronic phase degeneration of the postsynaptic folds was more intense than in the other chronic-phase animals. The posysynaptic membrane length and length per unit area and the MEPP amplitudes were significantly decreased in all chronic phase animals and the decreases were greater in the relapsing than in the non-lapsing animals. Minor morphometric alterations were also observed in the nerve terminals. These might have been secondary to the postsynaptic changes. The postsynaptic region is the primary target of the autoimmune reaction in EAMG. The ultrastructural, morphometric and electrophysiological abnormalities of the end-plate in chronic EAMG resemble those which have been observed in human myasthenia gravis.", "contents": "Experimental autoimmune myasthenia gravis: a sequential and quantitative study of the neuromuscular junction ultrastructure and electrophysiologic correlations. Neuromuscular junction ultrastructure in rat forelimb digit extensor muscle was sequentially and quantitatively investigated in experimental autoimmune myasthenia gravis (EAMG). Experimental animals were immunized with highly purified eel electroplax acetylcholine receptor protein plus complete Freund's adjuvant and B pertussis vaccine; control animals received only adjuvant and vaccine. During the first 7 days (latent period) after immunization end-plate structure and neuromuscular transmission remained normal in the experimental group. Between day 7 and 11 (acute phase) mononuclear cells infiltrated those regions of muscle where the end-plates were located and there was intense degeneration of the postsynaptic regions with splitting away of abnormal junctional folds from the underlying muscle fibers. Macrophages entered the gaps thus formed and removed the degenerating folds by phagocytosis. The nerve terminals were displaced from their usual location but maintained their structural integrity. Neuromuscular transmission was blocked in many muscle fibers. Miniature end-plate potentias (MEPPs), detectable in only a few fibers, were of abnormally low amplitude. After day 11 (chronic phase) the nerve terminals returned to the highly simplified postsynaptic folds became reconstituted and again degenerated. Immature junctions with poorly differentiated postsynaptic regions and nerve sprouts near end-plates were also observed. In two animals relapsing during the chronic phase degeneration of the postsynaptic folds was more intense than in the other chronic-phase animals. The posysynaptic membrane length and length per unit area and the MEPP amplitudes were significantly decreased in all chronic phase animals and the decreases were greater in the relapsing than in the non-lapsing animals. Minor morphometric alterations were also observed in the nerve terminals. These might have been secondary to the postsynaptic changes. The postsynaptic region is the primary target of the autoimmune reaction in EAMG. The ultrastructural, morphometric and electrophysiological abnormalities of the end-plate in chronic EAMG resemble those which have been observed in human myasthenia gravis.", "PMID": 956872}
{"id": "pubmed23n0032_2449", "title": "Ultrastructural evidence for CSF production by a choroid plexus papilloma.", "content": "A choroid plexus papilloma (CPP) removed from a child with communicating hydrocephalus was studied with the electron microscope. In addition to the usual organelles, the neoplastic epithelial cells contained polypoid microvilli, cilia, coated pinocytotic vesicles, and apical tight junctions. The basal surfaces were consistently juxtaposed to large capillaries lined by fenestrated endothelium. The fine structural details of the tumor were essentially the same as those of normal CP and thus appeared ideally suited for an active secretory function. Two additional features in this tumor were tubular bodies in the endothelium and well developed pericytes. The present findings provide strong evidence for cerebrospinal fluid production by CPP, a phenomenon that has long been suggested by clinical observation.", "contents": "Ultrastructural evidence for CSF production by a choroid plexus papilloma. A choroid plexus papilloma (CPP) removed from a child with communicating hydrocephalus was studied with the electron microscope. In addition to the usual organelles, the neoplastic epithelial cells contained polypoid microvilli, cilia, coated pinocytotic vesicles, and apical tight junctions. The basal surfaces were consistently juxtaposed to large capillaries lined by fenestrated endothelium. The fine structural details of the tumor were essentially the same as those of normal CP and thus appeared ideally suited for an active secretory function. Two additional features in this tumor were tubular bodies in the endothelium and well developed pericytes. The present findings provide strong evidence for cerebrospinal fluid production by CPP, a phenomenon that has long been suggested by clinical observation.", "PMID": 956877}
{"id": "pubmed23n0032_2450", "title": "Experience with Arnold-Chiari malformation, 1960 to 1970.", "content": "A retrospective study of 60 adult patients with Arnold-Chiari malformation revealed that certain presenting clinical syndromes, although not pathognomonic, seemed to have definite prognostic significance. Surgical management by suboccipital decompression led to remarkable and enduring improvement in 65% of patients followed for as long as 14 years. In some patients, however, the initial postoperative benefit tended to fade into an insidious progression of neurological deficit. Despite operation, 18.6% of patients eventually experienced progressive neurological deterioration. Patients who presented with paroxysmal intracranial hypertension or cerebellar dysfunction had the best prognosis. Evidence of central cord involvement was the single most detrimental factor to neurological recovery.", "contents": "Experience with Arnold-Chiari malformation, 1960 to 1970. A retrospective study of 60 adult patients with Arnold-Chiari malformation revealed that certain presenting clinical syndromes, although not pathognomonic, seemed to have definite prognostic significance. Surgical management by suboccipital decompression led to remarkable and enduring improvement in 65% of patients followed for as long as 14 years. In some patients, however, the initial postoperative benefit tended to fade into an insidious progression of neurological deficit. Despite operation, 18.6% of patients eventually experienced progressive neurological deterioration. Patients who presented with paroxysmal intracranial hypertension or cerebellar dysfunction had the best prognosis. Evidence of central cord involvement was the single most detrimental factor to neurological recovery.", "PMID": 956878}
{"id": "pubmed23n0032_2451", "title": "Restricted fluid intake. Rational management of the neurosurgical patient.", "content": "Water balance studies in postcraniotomy patients indicate that restriction of fluid intake to 1 liter daily maintains the patient in homeostatic balance. A larger fluid intake will expand the extracellular space and presumably unfavorably influence cerebral edema. Daily observation of serum sodium and osmolarity and blood urea nitrogen, and preserving their normalcy, is a rational way of regulating fluid intake of the brain-injured patient. Fluid restriction should be used with caution if hyperosmolar agents, diuretics, or dexamethasone are also administered.", "contents": "Restricted fluid intake. Rational management of the neurosurgical patient. Water balance studies in postcraniotomy patients indicate that restriction of fluid intake to 1 liter daily maintains the patient in homeostatic balance. A larger fluid intake will expand the extracellular space and presumably unfavorably influence cerebral edema. Daily observation of serum sodium and osmolarity and blood urea nitrogen, and preserving their normalcy, is a rational way of regulating fluid intake of the brain-injured patient. Fluid restriction should be used with caution if hyperosmolar agents, diuretics, or dexamethasone are also administered.", "PMID": 956879}
{"id": "pubmed23n0032_2452", "title": "Meningiomas causing spontaneous intracranial hematomas.", "content": "Four cases of spontaneous intracranial hematomas caused by unsuspected meningiomas are reported. Nine previous cases were found in the literature and the entire series of 13 cases is reviewed. Because of their unusual clinical presentation, these cases were not diagnosed correctly prior to radiological studies. Hypertension, trauma, and blood dyscrasia played no significant pathogenetic role. The histological features of these hemorrhagic meningiomas are described, and the importance of adequate radiological investigations and early radical surgery is stressed.", "contents": "Meningiomas causing spontaneous intracranial hematomas. Four cases of spontaneous intracranial hematomas caused by unsuspected meningiomas are reported. Nine previous cases were found in the literature and the entire series of 13 cases is reviewed. Because of their unusual clinical presentation, these cases were not diagnosed correctly prior to radiological studies. Hypertension, trauma, and blood dyscrasia played no significant pathogenetic role. The histological features of these hemorrhagic meningiomas are described, and the importance of adequate radiological investigations and early radical surgery is stressed.", "PMID": 956880}
{"id": "pubmed23n0032_2453", "title": "Reopening some occluded carotid arteries. Report of four cases.", "content": "Four cases are presented in which intermittent ischemic symptomatology was referable to a cerebral hemisphere. Arteriography in each case showed complete occlusion of the ipsilateral internal carotid artery in the neck with collateral filling of the ipsilateral intracranial carotid artery into the cavernous portion. Endarterectomy was performed successfully in each case from 1 to 5 weeks after the demonstrated complete occlusion. The collateral circulation to the intracranial portion of the internal carotid artery and the probable reasons for the technical success of these operations are discussed.", "contents": "Reopening some occluded carotid arteries. Report of four cases. Four cases are presented in which intermittent ischemic symptomatology was referable to a cerebral hemisphere. Arteriography in each case showed complete occlusion of the ipsilateral internal carotid artery in the neck with collateral filling of the ipsilateral intracranial carotid artery into the cavernous portion. Endarterectomy was performed successfully in each case from 1 to 5 weeks after the demonstrated complete occlusion. The collateral circulation to the intracranial portion of the internal carotid artery and the probable reasons for the technical success of these operations are discussed.", "PMID": 956881}
{"id": "pubmed23n0032_2454", "title": "Treatment of hydrocephalus and bilateral subdural effusions in a patient with closed sutures. Case report.", "content": "For management of bilateral subdural hematomas in a hydrocephalic child with fused sutures, a simple technique is described that involves minimal cranial procedures for modification of an already installed ventriculoperitoneal shunt system.", "contents": "Treatment of hydrocephalus and bilateral subdural effusions in a patient with closed sutures. Case report. For management of bilateral subdural hematomas in a hydrocephalic child with fused sutures, a simple technique is described that involves minimal cranial procedures for modification of an already installed ventriculoperitoneal shunt system.", "PMID": 956882}
{"id": "pubmed23n0032_2455", "title": "Persistent hypoglossal artery associated with aneurysms. Report of two cases.", "content": "Two cases of persistent hypoglossal artery associated with aneurysm are presented. In one case the aneurysm arose from the anterior communicating artery and in the other from the persistent hypoglossal artery itself. Both aneurysms were treated successfully with direct surgery.", "contents": "Persistent hypoglossal artery associated with aneurysms. Report of two cases. Two cases of persistent hypoglossal artery associated with aneurysm are presented. In one case the aneurysm arose from the anterior communicating artery and in the other from the persistent hypoglossal artery itself. Both aneurysms were treated successfully with direct surgery.", "PMID": 956883}
{"id": "pubmed23n0032_2456", "title": "Value of routine cerebral radionuclide angiography in pediatric brain imaging.", "content": "In addition to static brain images, cerebral radionuclide angiograms (CRAGs) were performed in 1,051 children to determine the value of routine radionuclide angiography. The CRAG resulted in a statistically significant increase in detection of abnormalities (p less than 0.01), as it provided the only evidence of confirmed abnormalities in 60 children (5.7%). The CRAG helped detect subdural fluid collections, cerebrovascular disease, and cerebral cysts, but it was of little value in detecting hydrocephalus. For maximum diagnostic yield, a CRAG should be performed with all pediatric brain-imaging studies.", "contents": "Value of routine cerebral radionuclide angiography in pediatric brain imaging. In addition to static brain images, cerebral radionuclide angiograms (CRAGs) were performed in 1,051 children to determine the value of routine radionuclide angiography. The CRAG resulted in a statistically significant increase in detection of abnormalities (p less than 0.01), as it provided the only evidence of confirmed abnormalities in 60 children (5.7%). The CRAG helped detect subdural fluid collections, cerebrovascular disease, and cerebral cysts, but it was of little value in detecting hydrocephalus. For maximum diagnostic yield, a CRAG should be performed with all pediatric brain-imaging studies.", "PMID": 956891}
{"id": "pubmed23n0032_2457", "title": "Routine renal imaging after 99mTc-glucoheptonate brain scans.", "content": "Immediately after brain scanning with 99mTc-glucoheptonate, the kidneys were imaged in 200 prospectively studied cases. Abnormalities were found in 22 cases (11%); they included renal metastases, renal cysts, and kidney displacement or obstruction by masses. In five instances, significant previously unknown abnormalities were found. The renal contours were usually better seen than on intravenous urograms or bone scans. Most kidney studies could be completed in less than 2 min, making renal imaging worthwhile as a low-cost high-yield routine addition to brain scanning.", "contents": "Routine renal imaging after 99mTc-glucoheptonate brain scans. Immediately after brain scanning with 99mTc-glucoheptonate, the kidneys were imaged in 200 prospectively studied cases. Abnormalities were found in 22 cases (11%); they included renal metastases, renal cysts, and kidney displacement or obstruction by masses. In five instances, significant previously unknown abnormalities were found. The renal contours were usually better seen than on intravenous urograms or bone scans. Most kidney studies could be completed in less than 2 min, making renal imaging worthwhile as a low-cost high-yield routine addition to brain scanning.", "PMID": 956892}
{"id": "pubmed23n0032_2458", "title": "Persistent dural cerebrospinal fluid leak shown by retrograde radionuclide myelography: case report.", "content": "Following inadvertent spinal anesthesia for delivery, a patient developed incapacitating post-lumbar puncture headache that persisted for 9 weeks. Scintigrams of the lumbar region, obtained after injection of 99mTC-human serum albumin into the cisterna magna, showed the cerebrospinal fluid leak. Blood patch repair was carried out, with immediate relief of all symptoms. Because of subsequent atypical headaches, a second cisternogram was done by the same technique. This study confirmed that there was no further dural leak, and other evidence indicated that the recurrent headache was related to functional problems.", "contents": "Persistent dural cerebrospinal fluid leak shown by retrograde radionuclide myelography: case report. Following inadvertent spinal anesthesia for delivery, a patient developed incapacitating post-lumbar puncture headache that persisted for 9 weeks. Scintigrams of the lumbar region, obtained after injection of 99mTC-human serum albumin into the cisterna magna, showed the cerebrospinal fluid leak. Blood patch repair was carried out, with immediate relief of all symptoms. Because of subsequent atypical headaches, a second cisternogram was done by the same technique. This study confirmed that there was no further dural leak, and other evidence indicated that the recurrent headache was related to functional problems.", "PMID": 956893}
{"id": "pubmed23n0032_2459", "title": "Tissue distribution of 203 Pb-acetate: comparison with 67 Ga-citrate as an abscess-localizing agent.", "content": "Since 203Pb-acetate accumulates in necrotic tumor tissue, the possibility was raised that it might also accumulate in other necrotic tissue such as abscess. We first studied the tissue distribution and excretion of 203Pb-acetate in control rats at 4, 24, 48, 72, and 96 hr. An enterohepatic circulation for lead is suggested. We then compared the uptakes of 203Pb-acetate and 67Ga-citrate in experimental abscesses in rats. The mean gallium accumulation in the abscess was 10 times that of lead at 24 hr and 12 times that of lead at 72 hr. The abscess-to-tissue ratios were greater for gallium for every tissue examined, although the abscessed areas were clearly visualized by scanning at 24 and 72 hr with both agents. With the exception of blood, abscess-to-tissue ratios for 67Ga at 24 hr were higher than or equal to those at 72 hr. However, the 67Ga ratios for the inflamed tissue surrounding the abscess to muscle and blood were higher at 72 hr than at 24 hr, which suggests that inflammation without abscess might be better identified by gallium scanning at 72 hr.", "contents": "Tissue distribution of 203 Pb-acetate: comparison with 67 Ga-citrate as an abscess-localizing agent. Since 203Pb-acetate accumulates in necrotic tumor tissue, the possibility was raised that it might also accumulate in other necrotic tissue such as abscess. We first studied the tissue distribution and excretion of 203Pb-acetate in control rats at 4, 24, 48, 72, and 96 hr. An enterohepatic circulation for lead is suggested. We then compared the uptakes of 203Pb-acetate and 67Ga-citrate in experimental abscesses in rats. The mean gallium accumulation in the abscess was 10 times that of lead at 24 hr and 12 times that of lead at 72 hr. The abscess-to-tissue ratios were greater for gallium for every tissue examined, although the abscessed areas were clearly visualized by scanning at 24 and 72 hr with both agents. With the exception of blood, abscess-to-tissue ratios for 67Ga at 24 hr were higher than or equal to those at 72 hr. However, the 67Ga ratios for the inflamed tissue surrounding the abscess to muscle and blood were higher at 72 hr than at 24 hr, which suggests that inflammation without abscess might be better identified by gallium scanning at 72 hr.", "PMID": 956894}
{"id": "pubmed23n0032_2460", "title": "Preparation of a chemically characterized 99mTc-penicillamine complex.", "content": "In the reaction labeling penicillamine with 99mTc, serveral different chemical states of 99mTc were observed to occur with slight changes in the labeling conditions, such as the concentrations of penicillamine, 99mTcO4-, stannous chloride, and hydrogen ion. We have studied the effects of these conditions on the formation of the monomer complex that we call Complex I, in which 99mTc is coordinated with the penicillamine as 99mTcO+2. Our approach is based on the thesis that Complex I formation competes with 99mTcO+2 hydrolysis, which leads to other 99mTc complexes. On this basis, the specific labeling conditions for Complex I formation have been established. Highly reproducible in vivo behavior in mice and rats was obtained with this chemically controlled Complex I.", "contents": "Preparation of a chemically characterized 99mTc-penicillamine complex. In the reaction labeling penicillamine with 99mTc, serveral different chemical states of 99mTc were observed to occur with slight changes in the labeling conditions, such as the concentrations of penicillamine, 99mTcO4-, stannous chloride, and hydrogen ion. We have studied the effects of these conditions on the formation of the monomer complex that we call Complex I, in which 99mTc is coordinated with the penicillamine as 99mTcO+2. Our approach is based on the thesis that Complex I formation competes with 99mTcO+2 hydrolysis, which leads to other 99mTc complexes. On this basis, the specific labeling conditions for Complex I formation have been established. Highly reproducible in vivo behavior in mice and rats was obtained with this chemically controlled Complex I.", "PMID": 956895}
{"id": "pubmed23n0032_2461", "title": "Technetium-99m-kethoxal-bis(thiosemicarbazone), an uncharged complex with a tetravalent 99mTc state, and its excretion into the bile.", "content": "Technetium-99m-kethoxal-bis (thiosemicarbazone) complex (99mTc-KTS), a potentially useful radiopharmaceutical for cholescintigraphy, is prepared by the reaction of KTS with 99mTcO4- in the presence of stannous chloride. Under the described conditions, a complex extractable with organic solvent was detected. Thin-layer chromatography, electrophoresis, and spectrometric analysis indicate the formation of an uncharged 99mTc(IV) complex. Organ distribution and biliary excretion studies are described. The complex showed a marked highly reproducible accumulation in the gallbladder.", "contents": "Technetium-99m-kethoxal-bis(thiosemicarbazone), an uncharged complex with a tetravalent 99mTc state, and its excretion into the bile. Technetium-99m-kethoxal-bis (thiosemicarbazone) complex (99mTc-KTS), a potentially useful radiopharmaceutical for cholescintigraphy, is prepared by the reaction of KTS with 99mTcO4- in the presence of stannous chloride. Under the described conditions, a complex extractable with organic solvent was detected. Thin-layer chromatography, electrophoresis, and spectrometric analysis indicate the formation of an uncharged 99mTc(IV) complex. Organ distribution and biliary excretion studies are described. The complex showed a marked highly reproducible accumulation in the gallbladder.", "PMID": 956896}
{"id": "pubmed23n0032_2462", "title": "Gonadal radiation dose and its genetic significance in radioiodine therapy of hyperthyroidism.", "content": "Published estimates of radiation dose to the gonads from 131I therapy of Graves' disease vary widely, largely because of differences in assumptions regarding the details of iodine kinetics. The calculations described in this paper show that hyperthyroid patients treated with 10 mCi of 131I will usually receive a total radiation dose to the ovaries or testes of less than 3 rad. Several common roentgenographic diagnostic procedures may involve a greater radiation dose and a greater genetic hazard than does the usual 131I treatment for hyperthyroidism. It is important to minimize total exposure to radiation, but it seems unreasonable to deny 131I treatment for hyperthyroidism to young men and nonpregnant young women on the grounds of genetic hazard alone.", "contents": "Gonadal radiation dose and its genetic significance in radioiodine therapy of hyperthyroidism. Published estimates of radiation dose to the gonads from 131I therapy of Graves' disease vary widely, largely because of differences in assumptions regarding the details of iodine kinetics. The calculations described in this paper show that hyperthyroid patients treated with 10 mCi of 131I will usually receive a total radiation dose to the ovaries or testes of less than 3 rad. Several common roentgenographic diagnostic procedures may involve a greater radiation dose and a greater genetic hazard than does the usual 131I treatment for hyperthyroidism. It is important to minimize total exposure to radiation, but it seems unreasonable to deny 131I treatment for hyperthyroidism to young men and nonpregnant young women on the grounds of genetic hazard alone.", "PMID": 956897}
{"id": "pubmed23n0032_2463", "title": "Estimates of radiation dose to the embryo from nuclear medicine procedures.", "content": "These phantom studies and computer calculations provide a direct means of estimating the radiation dose to the embryo resulting from the administration of a radiopharmaceutical to the mother during organogenesis. The specific absorbed fractions to the embryo from 19 source organs were computed for 12 monoenergetic photon energies. Tables of absorbed dose per unit cumulated activity, S, for the embryo as a target organ have been assembled for 99mTc, 111In, 113mIn, 123I, 131I, and 133Xe. In addition, the dose to the embryo was calculated for several of the radiopharmaceuticals for which the MIRD Committee has published dose estimate reports.", "contents": "Estimates of radiation dose to the embryo from nuclear medicine procedures. These phantom studies and computer calculations provide a direct means of estimating the radiation dose to the embryo resulting from the administration of a radiopharmaceutical to the mother during organogenesis. The specific absorbed fractions to the embryo from 19 source organs were computed for 12 monoenergetic photon energies. Tables of absorbed dose per unit cumulated activity, S, for the embryo as a target organ have been assembled for 99mTc, 111In, 113mIn, 123I, 131I, and 133Xe. In addition, the dose to the embryo was calculated for several of the radiopharmaceuticals for which the MIRD Committee has published dose estimate reports.", "PMID": 956898}
{"id": "pubmed23n0032_2464", "title": "Contamination from 131I, 103Ru, and 239Np in the eluate of 99Mo-99mTc generators loaded with (n, gamma)-produced 99Mo.", "content": "Iodine-131, ruthenium-103, and neptunium-239 are present as contaminants in the eluate of 99Mo-99mTc generators loaded with 99Mo prepared by thermal-neutron irradiation of enriched 98Mo. The elution pattern of each of these contaminants is determined, together with the amounts found in the eluate of all generators tested over a 7-month period.", "contents": "Contamination from 131I, 103Ru, and 239Np in the eluate of 99Mo-99mTc generators loaded with (n, gamma)-produced 99Mo. Iodine-131, ruthenium-103, and neptunium-239 are present as contaminants in the eluate of 99Mo-99mTc generators loaded with 99Mo prepared by thermal-neutron irradiation of enriched 98Mo. The elution pattern of each of these contaminants is determined, together with the amounts found in the eluate of all generators tested over a 7-month period.", "PMID": 956899}
{"id": "pubmed23n0032_2465", "title": "Digital low-pass filtering.", "content": "Random noise beyond the maximum signal frequency of the imaging system can be removed by low-pass filtering without compromising signal transmission.", "contents": "Digital low-pass filtering. Random noise beyond the maximum signal frequency of the imaging system can be removed by low-pass filtering without compromising signal transmission.", "PMID": 956900}
{"id": "pubmed23n0032_2466", "title": "Iodine-125-digoxin radioimmunoassay: comparison of commercial kits.", "content": "Iodine-125-digoxin radioimmunoassay kits available from Abbott Diagnostics (AD), Dade Division (D), Schwarz/Mann (SM), and Clinical Assays (CA) were evaluated with respect to assay quality. The kit accuracies did not differ significantly at 2.0 ng/ml and the interassay coefficients of variation ranged from 9% (AD) to 21.4% (CA) The accuracy for all kits above 4 ng/ml is questionable, and since serum-dilution values correlated well with undiluted serum values, the dilution method of dose quantitation is preferable for levels above 4 ng/ml. Although all the kits were adequate, for evaluating digoxin at the 2ng/ml level, the Abott kit seems to be of slightly better quality.", "contents": "Iodine-125-digoxin radioimmunoassay: comparison of commercial kits. Iodine-125-digoxin radioimmunoassay kits available from Abbott Diagnostics (AD), Dade Division (D), Schwarz/Mann (SM), and Clinical Assays (CA) were evaluated with respect to assay quality. The kit accuracies did not differ significantly at 2.0 ng/ml and the interassay coefficients of variation ranged from 9% (AD) to 21.4% (CA) The accuracy for all kits above 4 ng/ml is questionable, and since serum-dilution values correlated well with undiluted serum values, the dilution method of dose quantitation is preferable for levels above 4 ng/ml. Although all the kits were adequate, for evaluating digoxin at the 2ng/ml level, the Abott kit seems to be of slightly better quality.", "PMID": 956901}
{"id": "pubmed23n0032_2467", "title": "Dietary influence on bile acid conjugation in the cat.", "content": "Cats fed a semipurified diet containing casein as the source of protein develop taurine deficiency. In order to establish whether this had an adverse effect on bile, kittens and adult cats were fed the casein diet or that diet with a supplement of taurine, cystine, or methionine, and gall bladder bile was characterized for its taurine-glycine conjugation and the cholesterol: phospholipid: bile acid ratio. The data indicate that cats conjugate their bile acids almost exclusively with taurine, and though plasma and retinal pools of taurine are largely depleted by feeding the casein diet, the conjugation of bile acids is only moderately affected. A major conversion to glycine conjugation did not occur, but free cholic acid did increase with decreased taurine conjugation. Dietary supplements of methionine or cystine, precursors of taurine, failed to satisfy the taurine requirement for bile acids in kittens whereas methionine appeared to satisfy this requirement in adult cats. The cholesterol: phospholipid: bile acid profile was not appreciably altered by these dietary circumstances. It would appear that kittens may require dietary taurine and that taurine pools in cats may be biologically independent of one another.", "contents": "Dietary influence on bile acid conjugation in the cat. Cats fed a semipurified diet containing casein as the source of protein develop taurine deficiency. In order to establish whether this had an adverse effect on bile, kittens and adult cats were fed the casein diet or that diet with a supplement of taurine, cystine, or methionine, and gall bladder bile was characterized for its taurine-glycine conjugation and the cholesterol: phospholipid: bile acid ratio. The data indicate that cats conjugate their bile acids almost exclusively with taurine, and though plasma and retinal pools of taurine are largely depleted by feeding the casein diet, the conjugation of bile acids is only moderately affected. A major conversion to glycine conjugation did not occur, but free cholic acid did increase with decreased taurine conjugation. Dietary supplements of methionine or cystine, precursors of taurine, failed to satisfy the taurine requirement for bile acids in kittens whereas methionine appeared to satisfy this requirement in adult cats. The cholesterol: phospholipid: bile acid profile was not appreciably altered by these dietary circumstances. It would appear that kittens may require dietary taurine and that taurine pools in cats may be biologically independent of one another.", "PMID": 956906}
{"id": "pubmed23n0032_2468", "title": "Decreased plasma insulin but normal glucagon in rats fed low protein diets.", "content": "Plasma glucagon and insulin were determined in rats fed three diets, one control and two low protein (LP 1 & LP 2). In LP 1, the protein omitted was replaced by carbohydrate while in LP 2, fat and alpha-cellulose replaced the omitted protein. Among rats fed LP diets ad libitum, food consumption decreased and body weight loss occurred. In order to separate the effects of reduced food intake and weight loss from the effects of LP diet alone, paired feeding and paired weight experiments were conducted. In another experiment, ingestion of a LP diet for 8 to 10 days was followed by refeeding the control diet for 5 days. The results demonstrate that plasma insulin was reduced in LP rats compared to the full-fed controls and pair-fed controls, the lowest levels being observed in rats fed LP 2 diet. Furthermore, the paired feeding experiment revealed that the diminished food consumption plays no significant role in lowering plasma insulin in LP rats. The refeeding experiment showed that the decline in plasma insulin in LP rats is a transient phenomenon and the plasma insulin promptly reverts to normal upon resumption of feeding the control diet. Plasma glucagon was unaltered throughout these dietary manipulations.", "contents": "Decreased plasma insulin but normal glucagon in rats fed low protein diets. Plasma glucagon and insulin were determined in rats fed three diets, one control and two low protein (LP 1 & LP 2). In LP 1, the protein omitted was replaced by carbohydrate while in LP 2, fat and alpha-cellulose replaced the omitted protein. Among rats fed LP diets ad libitum, food consumption decreased and body weight loss occurred. In order to separate the effects of reduced food intake and weight loss from the effects of LP diet alone, paired feeding and paired weight experiments were conducted. In another experiment, ingestion of a LP diet for 8 to 10 days was followed by refeeding the control diet for 5 days. The results demonstrate that plasma insulin was reduced in LP rats compared to the full-fed controls and pair-fed controls, the lowest levels being observed in rats fed LP 2 diet. Furthermore, the paired feeding experiment revealed that the diminished food consumption plays no significant role in lowering plasma insulin in LP rats. The refeeding experiment showed that the decline in plasma insulin in LP rats is a transient phenomenon and the plasma insulin promptly reverts to normal upon resumption of feeding the control diet. Plasma glucagon was unaltered throughout these dietary manipulations.", "PMID": 956907}
{"id": "pubmed23n0032_2469", "title": "Teratogenic effects of magnesium deficiency in rats.", "content": "The effect of severe magnesium deficiency on plasma magnesium levels in pregnant and nonpregnant rats and on reproduction was investigated. Magnesium deficiency produced a rapid fall in plasma magnesium concentration, in both pregnant and nonpregnant rats. At term, all implantation sites of pregnant rats fed a diet severely deficient in magnesium showed total fetal resorption. When pregnant females were fed the magnesium deficient diet only between days 6 and 14 of gestation, there was a high incidence of resorptions and gross malformations were seen in full term fetuses. The results demonstrate the rapidity of the effects of severe magnesium deficiency in pregnant rats and indicate the importance of the element for embryonic development.", "contents": "Teratogenic effects of magnesium deficiency in rats. The effect of severe magnesium deficiency on plasma magnesium levels in pregnant and nonpregnant rats and on reproduction was investigated. Magnesium deficiency produced a rapid fall in plasma magnesium concentration, in both pregnant and nonpregnant rats. At term, all implantation sites of pregnant rats fed a diet severely deficient in magnesium showed total fetal resorption. When pregnant females were fed the magnesium deficient diet only between days 6 and 14 of gestation, there was a high incidence of resorptions and gross malformations were seen in full term fetuses. The results demonstrate the rapidity of the effects of severe magnesium deficiency in pregnant rats and indicate the importance of the element for embryonic development.", "PMID": 956908}
{"id": "pubmed23n0032_2470", "title": "Magnesium, calcium and zinc levels of maternal and fetal tissues in magnesium deficient rats.", "content": "The interaction of magnesium with two other mineral elements, calcium and zinc, was studied in maternal tissues and fetuses of pregnant rats fed a magnesium deficient diet throughout gestation. Reduction in maternal femur magnesium and a trend for increased kidney calcium reflected the dietary magnesium deficiency. In fetuses, however, total magnesium content was reduced and , in addition, fetal zinc content was also lower than normal in the group most severly deficient in magnesium. The possible interpretation of this magnesium-zinc interaction is discussed.", "contents": "Magnesium, calcium and zinc levels of maternal and fetal tissues in magnesium deficient rats. The interaction of magnesium with two other mineral elements, calcium and zinc, was studied in maternal tissues and fetuses of pregnant rats fed a magnesium deficient diet throughout gestation. Reduction in maternal femur magnesium and a trend for increased kidney calcium reflected the dietary magnesium deficiency. In fetuses, however, total magnesium content was reduced and , in addition, fetal zinc content was also lower than normal in the group most severly deficient in magnesium. The possible interpretation of this magnesium-zinc interaction is discussed.", "PMID": 956909}
{"id": "pubmed23n0032_2471", "title": "Enhancement of Ca++ uptake by lactose in the rat small intestine.", "content": "In previous experiments, lactose was shown to increase the absorption of Ca++ by the small intestine of the rat and other mammals. To further investigate the mechanism of the lactose effect, Ca++ uptake was studied in everted gut sac preparations. Gut sacs from the rat ileum were preincubated with or without lactose for 45 minutes, and then the tissue uptake of 45Ca over the first 3 minutes was measured in the presence or absence of lactose. The presence of 160 mM lactose increased the initial rate of Ca++ uptake in the first minute by 64% compared to the NaCl control. The lactose effect was dependent on the presence of lactose in the preincubation medium only and not on the presence of lactose during the measurement of Ca++ uptake. Lactose increased Ca++ absorption when the Ca++ concentrations ranged from 0.1 to 10 mM. However, the magnitude of the enhancement was dependent on the lactose concentration and was reduced below 160 mM lactose. When Ca++ and lactose uptake during a 45 minute period was measured in parallel experiments, no evidence for the co-transport of lactose and Ca++ into the tissue was found. These and other data indicated that lactose is not interacting directly with Ca++ in solution but is interacting with the absorptive cells of the intestine to increase their permeability to Ca++.", "contents": "Enhancement of Ca++ uptake by lactose in the rat small intestine. In previous experiments, lactose was shown to increase the absorption of Ca++ by the small intestine of the rat and other mammals. To further investigate the mechanism of the lactose effect, Ca++ uptake was studied in everted gut sac preparations. Gut sacs from the rat ileum were preincubated with or without lactose for 45 minutes, and then the tissue uptake of 45Ca over the first 3 minutes was measured in the presence or absence of lactose. The presence of 160 mM lactose increased the initial rate of Ca++ uptake in the first minute by 64% compared to the NaCl control. The lactose effect was dependent on the presence of lactose in the preincubation medium only and not on the presence of lactose during the measurement of Ca++ uptake. Lactose increased Ca++ absorption when the Ca++ concentrations ranged from 0.1 to 10 mM. However, the magnitude of the enhancement was dependent on the lactose concentration and was reduced below 160 mM lactose. When Ca++ and lactose uptake during a 45 minute period was measured in parallel experiments, no evidence for the co-transport of lactose and Ca++ into the tissue was found. These and other data indicated that lactose is not interacting directly with Ca++ in solution but is interacting with the absorptive cells of the intestine to increase their permeability to Ca++.", "PMID": 956910}
{"id": "pubmed23n0032_2472", "title": "Fractors affecting the rate of gluconeogenesis from L-cysteine in the perfused rat liver.", "content": "It has been shown that the net rate of gluconeogenesis from cysteine was only 10% the rate observed from pyruvate. This suggested that the rate limiting step in gluconeogenesis from cysteine was between cysteine and pyruvate. Evidence is presented showing that the cysteine-sulfinate pathway does not play a regulating role in the conversion of cysteine to glucose. Thus, liver cysteine desulfhydrase (CDS) activity and hydrogen sulfide production were evaluated for their potential effects. Liver CDS activity was increased by a 3 day starvation, by feeding a 90% casein diet or a 4% cysteine + 86% casein diet. In all cases the activity of the enzyme was in excess of that required to account for the rate of conversion of cysteine to glucose observed, thus the potential activity of this enzyme was not a rate limiting factor. The possible effect of H2S, an end product of the CDS reaction, on gluconeogenesis from cysteine was evaluated. The addition of NaHS abolished the glucogenic response observed from cysteine, but had very little effect on glucoeogenesis from lactate, suggesting that accumulated H2S may inhibit CDS, marking CDS rate limiting in the conversion of cysteine to pyruvate.", "contents": "Fractors affecting the rate of gluconeogenesis from L-cysteine in the perfused rat liver. It has been shown that the net rate of gluconeogenesis from cysteine was only 10% the rate observed from pyruvate. This suggested that the rate limiting step in gluconeogenesis from cysteine was between cysteine and pyruvate. Evidence is presented showing that the cysteine-sulfinate pathway does not play a regulating role in the conversion of cysteine to glucose. Thus, liver cysteine desulfhydrase (CDS) activity and hydrogen sulfide production were evaluated for their potential effects. Liver CDS activity was increased by a 3 day starvation, by feeding a 90% casein diet or a 4% cysteine + 86% casein diet. In all cases the activity of the enzyme was in excess of that required to account for the rate of conversion of cysteine to glucose observed, thus the potential activity of this enzyme was not a rate limiting factor. The possible effect of H2S, an end product of the CDS reaction, on gluconeogenesis from cysteine was evaluated. The addition of NaHS abolished the glucogenic response observed from cysteine, but had very little effect on glucoeogenesis from lactate, suggesting that accumulated H2S may inhibit CDS, marking CDS rate limiting in the conversion of cysteine to pyruvate.", "PMID": 956911}
{"id": "pubmed23n0032_2473", "title": "Comparison of the binding of various bile acids and bile salts in vitro by several types of fiber.", "content": "The binding in vitro of the sodium salts of cholic acid, chenodeoxycholic acid, deoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, glycocholic acid, glycochenodeoxycholic acid, and glycodeoxycholic acid by alfalfa, bran, cellulose, lignin, and cholestyramine was measured. Cholestyramine bound an average of 81.3% of all the bile acids and salts tested whereas cellulose bound only negligible amounts (1.4%). Of the other substances tested, lignin bound 29.2%, alfalfa, 15.9% and bran, 9.0%. No distinct pattern of binding was discerned. It is therefore apparent that the validity of statements concerning the effect of fiber on bile salf metabolism rests upon the specificity of the composition of the fiber involved and the bile acids or salts tested.", "contents": "Comparison of the binding of various bile acids and bile salts in vitro by several types of fiber. The binding in vitro of the sodium salts of cholic acid, chenodeoxycholic acid, deoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, glycocholic acid, glycochenodeoxycholic acid, and glycodeoxycholic acid by alfalfa, bran, cellulose, lignin, and cholestyramine was measured. Cholestyramine bound an average of 81.3% of all the bile acids and salts tested whereas cellulose bound only negligible amounts (1.4%). Of the other substances tested, lignin bound 29.2%, alfalfa, 15.9% and bran, 9.0%. No distinct pattern of binding was discerned. It is therefore apparent that the validity of statements concerning the effect of fiber on bile salf metabolism rests upon the specificity of the composition of the fiber involved and the bile acids or salts tested.", "PMID": 956912}
{"id": "pubmed23n0032_2474", "title": "Changes in patterns of feeding activity, parotid secretory enzymes and plasma corticosterone in developing rats.", "content": "The diet and feeding patterns of developing rats were determined from feed bin weight losses and analysis of stomach contents (details in a previous report). Parotid gland development in the same rats was assessed from the specific activities of the secretory enzymes alpha-amylase, RNase and DNase, with particular attention to the occurrence of circadian variations. The results indicate that during the first week post partum, rats suckle much more by day than by night but have no circadian cycles in their parotid glands. Between 10 and 20 days, there were no consistent cycles either in feeding activity or in parotid enzymes. The progressive change from milk to stock diet between 14 and 30 days appears to promote the maturation of the parotid gland. The halfway point in the dietary change at 21 days coincides with the onset of inversely related circadian cycles in feeding and the parotid enzymes. Premature weaning at 21 days accentuates both cycles and accelerates parotid maturation. The findings indicate that the physical consistency of the diet has an imporant regulatory role in the developmental patterns of feeding activity and parotid glands of rats, but they also hint that other dietary qualities may be involved. Chronologic fluctuations in plasma corticosterone suggest an intricate relationship among this hormone, feeding behavior, and parotid glands in developing rats.", "contents": "Changes in patterns of feeding activity, parotid secretory enzymes and plasma corticosterone in developing rats. The diet and feeding patterns of developing rats were determined from feed bin weight losses and analysis of stomach contents (details in a previous report). Parotid gland development in the same rats was assessed from the specific activities of the secretory enzymes alpha-amylase, RNase and DNase, with particular attention to the occurrence of circadian variations. The results indicate that during the first week post partum, rats suckle much more by day than by night but have no circadian cycles in their parotid glands. Between 10 and 20 days, there were no consistent cycles either in feeding activity or in parotid enzymes. The progressive change from milk to stock diet between 14 and 30 days appears to promote the maturation of the parotid gland. The halfway point in the dietary change at 21 days coincides with the onset of inversely related circadian cycles in feeding and the parotid enzymes. Premature weaning at 21 days accentuates both cycles and accelerates parotid maturation. The findings indicate that the physical consistency of the diet has an imporant regulatory role in the developmental patterns of feeding activity and parotid glands of rats, but they also hint that other dietary qualities may be involved. Chronologic fluctuations in plasma corticosterone suggest an intricate relationship among this hormone, feeding behavior, and parotid glands in developing rats.", "PMID": 956913}
{"id": "pubmed23n0032_2475", "title": "Effects in the rat of environmental temperature, diet dilution and treadmill running on voluntary food intake, body composition and endocrine organ mass: a multiple regression analysis.", "content": "The effects upon weight gain, food intake, carcass composition, and weight of pituitary, adrenals, and testes of male rats subjected to three environmental temperatures (T; 16.0 degrees, 23.5 degrees, and 31.0 degrees), five diet dilution rates (D; 0%, 12.5% 25.0%, 37.5%, and 50.0%), and five daily treadmill running periods (E; 10 minutes, 40 minutes, 70 minutes, 100 minutes, and 130 minutes) were studied by multiple regression techniques. Equations describing regression surfaces could contain significant linear and/or quadratic coefficients of the independent variables (T, D, and E) and their first order interactions. Body weight attained was reduced by increased D and T. Total food intake increased linearly with increases in D, but this increase was smaller for the rats in the higher environmental temperature since there was a significant DT interaction. Total food intake was decreased by increases in T. The intake of food minus diluent was maintained until D attained a value of 37.5%; beyond this point the rats at all three temperatures failed to maintain digestible energy intake. The data strongly suggested that the energy intake was not limited by the gastrointestinal tract capacity. E did not significantly affect food intake. Significant regression coefficients were found for the dependent variables of body dry matter, nitrogen, ash, ether extract, and energy and of the weight of depot fat pads, adrenals, and testes, as follows: (1) For D, there was no linear coefficient for body nitrogen. All remaining linear coefficients were negative except for body ash. The only quadratic coefficients (both positive) were for adrenal and testes weights (2) For E, all linear coeffcients were negative except those for body nitrogen, body ash, and adrenal weight. There were not quadratic coefficients for body nitrogen, body ash, adrenal weight, and testes weight. The remaining quadratic coefficients were all positive; (3) For T, there was no quadratic coefficient for body energy. The remaining linear coefficients were positive except those for fat deposit size, body ether extract, and adrenal weight. The remaining quadratic coefficients were negative except those for fat deposit size, body ether extract, and adrenal weight; (4) For the DE interaction, no coefficients were significant; (5) For the DT interaction, there were no significant coefficients for body nitrogen, fat depot size, or adrenal weight. The remaining coefficients were positive except for body ash; (6) For the ET interaction, the only significant coefficients were negative ones for body ether extract and body energy and a positive one for testes weight.", "contents": "Effects in the rat of environmental temperature, diet dilution and treadmill running on voluntary food intake, body composition and endocrine organ mass: a multiple regression analysis. The effects upon weight gain, food intake, carcass composition, and weight of pituitary, adrenals, and testes of male rats subjected to three environmental temperatures (T; 16.0 degrees, 23.5 degrees, and 31.0 degrees), five diet dilution rates (D; 0%, 12.5% 25.0%, 37.5%, and 50.0%), and five daily treadmill running periods (E; 10 minutes, 40 minutes, 70 minutes, 100 minutes, and 130 minutes) were studied by multiple regression techniques. Equations describing regression surfaces could contain significant linear and/or quadratic coefficients of the independent variables (T, D, and E) and their first order interactions. Body weight attained was reduced by increased D and T. Total food intake increased linearly with increases in D, but this increase was smaller for the rats in the higher environmental temperature since there was a significant DT interaction. Total food intake was decreased by increases in T. The intake of food minus diluent was maintained until D attained a value of 37.5%; beyond this point the rats at all three temperatures failed to maintain digestible energy intake. The data strongly suggested that the energy intake was not limited by the gastrointestinal tract capacity. E did not significantly affect food intake. Significant regression coefficients were found for the dependent variables of body dry matter, nitrogen, ash, ether extract, and energy and of the weight of depot fat pads, adrenals, and testes, as follows: (1) For D, there was no linear coefficient for body nitrogen. All remaining linear coefficients were negative except for body ash. The only quadratic coefficients (both positive) were for adrenal and testes weights (2) For E, all linear coeffcients were negative except those for body nitrogen, body ash, and adrenal weight. There were not quadratic coefficients for body nitrogen, body ash, adrenal weight, and testes weight. The remaining quadratic coefficients were all positive; (3) For T, there was no quadratic coefficient for body energy. The remaining linear coefficients were positive except those for fat deposit size, body ether extract, and adrenal weight. The remaining quadratic coefficients were negative except those for fat deposit size, body ether extract, and adrenal weight; (4) For the DE interaction, no coefficients were significant; (5) For the DT interaction, there were no significant coefficients for body nitrogen, fat depot size, or adrenal weight. The remaining coefficients were positive except for body ash; (6) For the ET interaction, the only significant coefficients were negative ones for body ether extract and body energy and a positive one for testes weight.", "PMID": 956914}
{"id": "pubmed23n0032_2476", "title": "Controlling factors in the development of ceruloplasmin in pigs during the neonatal growth period.", "content": "The sequence of ceruloplasmin development and limiting factors controlling this process in neonatal piglets was studied in a randomized block design. Guided by the change in serum ceruloplasmin levels, the liver copper concentrations of piglets fed copper-deficient and copper-supplemented diets were compared in three different periods. The plasma of piglets was devoid of both apo- and holo-ceruloplasmin at birth. The system responsible for the synthesis of apoceruloplasmin developed shortly after birth as indicated by a steady increase in serum ceruloplasmin activity during the first week without being affected by the diet. Copper apparently was not the limiting factor at this stage since very high concentrations of copper were found in the livers of newborn piglets. However, the liver copper was depleted quickly within 1 to 2 weeks and became the limiting factor if the diet was deficient in copper. The livers of these piglets were fractionated by differential centrifugation to compare the liver copper distribution in the two dietary groups with and without copper supplementation. The treatments did not affect the copper distribution on a percentage of total liver copper basis. However, fractionation study revealed that, unlike adult pig livers, about 70% to 80% of copper was present in the heavier particle fractions of piglet livers.", "contents": "Controlling factors in the development of ceruloplasmin in pigs during the neonatal growth period. The sequence of ceruloplasmin development and limiting factors controlling this process in neonatal piglets was studied in a randomized block design. Guided by the change in serum ceruloplasmin levels, the liver copper concentrations of piglets fed copper-deficient and copper-supplemented diets were compared in three different periods. The plasma of piglets was devoid of both apo- and holo-ceruloplasmin at birth. The system responsible for the synthesis of apoceruloplasmin developed shortly after birth as indicated by a steady increase in serum ceruloplasmin activity during the first week without being affected by the diet. Copper apparently was not the limiting factor at this stage since very high concentrations of copper were found in the livers of newborn piglets. However, the liver copper was depleted quickly within 1 to 2 weeks and became the limiting factor if the diet was deficient in copper. The livers of these piglets were fractionated by differential centrifugation to compare the liver copper distribution in the two dietary groups with and without copper supplementation. The treatments did not affect the copper distribution on a percentage of total liver copper basis. However, fractionation study revealed that, unlike adult pig livers, about 70% to 80% of copper was present in the heavier particle fractions of piglet livers.", "PMID": 956915}
{"id": "pubmed23n0032_2477", "title": "Adipose tissue cell size and lipolysis in the rat: response to exercise intensity and food restriction.", "content": "This experiment was designed to determine if the adaptive increase in adipose tissue epinephrine-stimulated lipolysis (ESL) observed in exercise trained rats is related to decreased adipocyte size or a direct response to exercise. Two levels of treadmill exercise and three levels of food restriction were imposed on male rats over a 12 week experimental period to create a distribution of adipose tissue cell sizes. Epinephrine-stimulated lipolysis was subsequently measured in the isolated adipocytes from rats trained at two different exercise levels and in untrained rats fed either ad libitum or 16%, 27%, or 35% dietary restriction. Energy restriction was effective in reducing body weight and to some extent epididymal fat pad weight; however, adipocyte size and number were not significantly affected. Exercise in both groups of trained rats was effective in reducing adipocyte size; however, cell size did not differ between training groups. The group receiving the greatest amount of daily exercise had significantly greater ESL indicating that the adaptive increase in lipolytic potential seen in adipose tissue of exercise trained rats is a true metabolic adaptation not secondary to reduced cell size.", "contents": "Adipose tissue cell size and lipolysis in the rat: response to exercise intensity and food restriction. This experiment was designed to determine if the adaptive increase in adipose tissue epinephrine-stimulated lipolysis (ESL) observed in exercise trained rats is related to decreased adipocyte size or a direct response to exercise. Two levels of treadmill exercise and three levels of food restriction were imposed on male rats over a 12 week experimental period to create a distribution of adipose tissue cell sizes. Epinephrine-stimulated lipolysis was subsequently measured in the isolated adipocytes from rats trained at two different exercise levels and in untrained rats fed either ad libitum or 16%, 27%, or 35% dietary restriction. Energy restriction was effective in reducing body weight and to some extent epididymal fat pad weight; however, adipocyte size and number were not significantly affected. Exercise in both groups of trained rats was effective in reducing adipocyte size; however, cell size did not differ between training groups. The group receiving the greatest amount of daily exercise had significantly greater ESL indicating that the adaptive increase in lipolytic potential seen in adipose tissue of exercise trained rats is a true metabolic adaptation not secondary to reduced cell size.", "PMID": 956916}
{"id": "pubmed23n0032_2478", "title": "Amino acid requirements of the growing mouse.", "content": "Using diets based on rat amino acid requirements, containing 2.0% nitrogen and 4.1 digestible kcal/g, the dietary levels of ten essential amino acids required for normal post-weaning growth of Swiss X Carworth Farms No. 1 crossbred mice were estimated. The response criteria used were growth rate, dry matter intake, energy and protein contents of body weight gain, protein efficiency ratio and apparent biological value. Maximum responses were obtained when the dietary dry matter contained less than or equal 0.3% arginine, 0.2% histidine, 0.4% isoleucine, 0.7% leucine, less than or equal 0.4% lysine, 0.5% methionine, less than or equal 0.4% phenylalanine, 0.4% threonine, less than or equal 0.1% tryptophan and 0.5% valine.", "contents": "Amino acid requirements of the growing mouse. Using diets based on rat amino acid requirements, containing 2.0% nitrogen and 4.1 digestible kcal/g, the dietary levels of ten essential amino acids required for normal post-weaning growth of Swiss X Carworth Farms No. 1 crossbred mice were estimated. The response criteria used were growth rate, dry matter intake, energy and protein contents of body weight gain, protein efficiency ratio and apparent biological value. Maximum responses were obtained when the dietary dry matter contained less than or equal 0.3% arginine, 0.2% histidine, 0.4% isoleucine, 0.7% leucine, less than or equal 0.4% lysine, 0.5% methionine, less than or equal 0.4% phenylalanine, 0.4% threonine, less than or equal 0.1% tryptophan and 0.5% valine.", "PMID": 956917}
{"id": "pubmed23n0032_2479", "title": "Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus.", "content": "Twenty-one semipurified diets were formulated to determine the optimum protein:energy ratio (P/E) for channel catfish fingerlings. Seven crude protein levels and three energy levels at each protein level were utilized. The protein, lipid, and digestible carbohydrate sources were hexane-extracted whole egg powder, salmon-corn oil mixture, and white dextrin, respectively. After an initial 2 week conditioning period, the experimental diets were fed in triplicate to groups of 30 +/- 3 channel catfish fingerlings weighing 205 +/- 10 g and kept in 110 liter flow-through aquaria at 26.7 +/- 1.1 degrees. The diets were fed at a rate equalling 3% of the total wet body weight/day divided into three equal feedings and adjusted weekly for 9 weeks. An approximate fivefold increase in body weight was observed. These data indicate that the concept of P/E ratios must be restricted to diets containing adequate levels of protein and total energy. Based on average daily gain, diets containing energy levels between 275 and 341 kcal/100 g had an optimum P/E ratio of approximately 88 (24-36% crude protein). Diets containing 24% crude protein and 275 kcal/100 g diet appeared to be utilized more efficiently in terms of percent protein deposited than diets containing higher crude protein levels.", "contents": "Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. Twenty-one semipurified diets were formulated to determine the optimum protein:energy ratio (P/E) for channel catfish fingerlings. Seven crude protein levels and three energy levels at each protein level were utilized. The protein, lipid, and digestible carbohydrate sources were hexane-extracted whole egg powder, salmon-corn oil mixture, and white dextrin, respectively. After an initial 2 week conditioning period, the experimental diets were fed in triplicate to groups of 30 +/- 3 channel catfish fingerlings weighing 205 +/- 10 g and kept in 110 liter flow-through aquaria at 26.7 +/- 1.1 degrees. The diets were fed at a rate equalling 3% of the total wet body weight/day divided into three equal feedings and adjusted weekly for 9 weeks. An approximate fivefold increase in body weight was observed. These data indicate that the concept of P/E ratios must be restricted to diets containing adequate levels of protein and total energy. Based on average daily gain, diets containing energy levels between 275 and 341 kcal/100 g had an optimum P/E ratio of approximately 88 (24-36% crude protein). Diets containing 24% crude protein and 275 kcal/100 g diet appeared to be utilized more efficiently in terms of percent protein deposited than diets containing higher crude protein levels.", "PMID": 956918}
{"id": "pubmed23n0032_2480", "title": "Convulsive seizure induced by intracerebral injection of semicarbazide (an anti-vitamin B6) in the mouse.", "content": "The direct injection of semicarbazide (SC), an antivitamin B6 (anti-B6), into the lateral ventricle of the mouse brain induced convulsion and tremors at a smaller dose after a shorter latent period than that in systemic administration. The symptoms were prevented by pyridoxine, aminooxyacetic acid or acetone, while they enhanced by pyridoxal, pyridoxal phosphate, or some other anti-B6. In mice fed a vitamin B6 (B6)-deficient diet, convulsion and tremors occurred at smaller doses of SC than those in mice given control food, and were counteracted by pyridoxine. On the other hand, mice into which SC had been injected in the neighboring site of the lambda first showed running fits, which was followed by convulsion and tremors.", "contents": "Convulsive seizure induced by intracerebral injection of semicarbazide (an anti-vitamin B6) in the mouse. The direct injection of semicarbazide (SC), an antivitamin B6 (anti-B6), into the lateral ventricle of the mouse brain induced convulsion and tremors at a smaller dose after a shorter latent period than that in systemic administration. The symptoms were prevented by pyridoxine, aminooxyacetic acid or acetone, while they enhanced by pyridoxal, pyridoxal phosphate, or some other anti-B6. In mice fed a vitamin B6 (B6)-deficient diet, convulsion and tremors occurred at smaller doses of SC than those in mice given control food, and were counteracted by pyridoxine. On the other hand, mice into which SC had been injected in the neighboring site of the lambda first showed running fits, which was followed by convulsion and tremors.", "PMID": 956921}
{"id": "pubmed23n0032_2481", "title": "Cardiac action of thiamine derivatives in guinea pig atria.", "content": "Pharmacological studies were performed on allithiamine (TAD), thiamine propyl disulfide (TPD), and thiamine tetrahydrofurfuryl disulfide (TTFD) to investigate positive inotropic and negative chronotropic effects seen when they are applied to spontaneous beats in isolated guinea pig atria at concentrations higher than 10-5 g/ml. 1. The effects of thiamine 8-(methyl-6-acetyldihydrothioacetate) disulfide (TATD) and thiamine hydroxyethyl disulfide (TOED) at 5 x 10-4 were slight, and those of dibenzoyl thiamine (DBT) and thiamine were limited at any concentration. 2. Dimethyl propyl disulfide (DMPD) which has anti-thiamine activity, showed these effects at concentrations higher than 10-5. 3. The negative chronotropic effect of TAD, TPD, and TTFD was not influenced by the prior application of atropine, and the positive inotropic effect was not influenced by propranolol. 4. The effects of TTFD on the electrically driven left atrial muscle were remarkable when the muscle was driven at low frequency, while they were less remarkable at high frequency. 5. The decrease in tension of the electrically driven left atria induced by mersalyl at 5 x 10-4 g/ml was recovered by the subsequent addition of TTFD or TPD at 5 x 10-4 as well as dimercaprol at 5 x 10-5. From the results, it was assumed that (a) the effects of TAD, TPD and TTFD might relate to their common chemical structure of the disulfide, especially to the alkyldisulfide chain, (b) the effects are irrevelant to their common activity as an vitamin B1 and to either cholinergic or adrenergic effect, and (c) a mutual dependence is seen between the positive inotropic effect and the negative chronotropic effect.", "contents": "Cardiac action of thiamine derivatives in guinea pig atria. Pharmacological studies were performed on allithiamine (TAD), thiamine propyl disulfide (TPD), and thiamine tetrahydrofurfuryl disulfide (TTFD) to investigate positive inotropic and negative chronotropic effects seen when they are applied to spontaneous beats in isolated guinea pig atria at concentrations higher than 10-5 g/ml. 1. The effects of thiamine 8-(methyl-6-acetyldihydrothioacetate) disulfide (TATD) and thiamine hydroxyethyl disulfide (TOED) at 5 x 10-4 were slight, and those of dibenzoyl thiamine (DBT) and thiamine were limited at any concentration. 2. Dimethyl propyl disulfide (DMPD) which has anti-thiamine activity, showed these effects at concentrations higher than 10-5. 3. The negative chronotropic effect of TAD, TPD, and TTFD was not influenced by the prior application of atropine, and the positive inotropic effect was not influenced by propranolol. 4. The effects of TTFD on the electrically driven left atrial muscle were remarkable when the muscle was driven at low frequency, while they were less remarkable at high frequency. 5. The decrease in tension of the electrically driven left atria induced by mersalyl at 5 x 10-4 g/ml was recovered by the subsequent addition of TTFD or TPD at 5 x 10-4 as well as dimercaprol at 5 x 10-5. From the results, it was assumed that (a) the effects of TAD, TPD and TTFD might relate to their common chemical structure of the disulfide, especially to the alkyldisulfide chain, (b) the effects are irrevelant to their common activity as an vitamin B1 and to either cholinergic or adrenergic effect, and (c) a mutual dependence is seen between the positive inotropic effect and the negative chronotropic effect.", "PMID": 956922}
{"id": "pubmed23n0032_2482", "title": "Effects of low temperature and electrical (square wave) stimulation on spontaneous contractions in isolated guinea pig atria and influence of thiamine tetrahydrofurfuryl disulfide (TTFD) on these effects.", "content": "The spontaneous contractions of isolated guinea pig atria were arrested by a temperature change in the medium for 30 to 20 degrees C within 20 min. 2. When thiamine tetrahydrofurfuryl sulfide (TTFD) at concentration of 10-5 g/ml was added in the medium, the arrest was not seen for more than 30 min. 3. Arrhythmic contractions induced by the electrical square wave stimulation of threshold intensity were prevented by TTFD at 10-4 g/ml which was added to the medium. 4. These effects of TTFD at respective concentrations were seen even 30 min after the drug was removed from the medium, when the atria had been pre-incubated with the drug for one hour before the removal. 5. From these results, it was assumed that TTFD might show these effects against the extrinsic physical invasions through the stabilization of the tissue membrane.", "contents": "Effects of low temperature and electrical (square wave) stimulation on spontaneous contractions in isolated guinea pig atria and influence of thiamine tetrahydrofurfuryl disulfide (TTFD) on these effects. The spontaneous contractions of isolated guinea pig atria were arrested by a temperature change in the medium for 30 to 20 degrees C within 20 min. 2. When thiamine tetrahydrofurfuryl sulfide (TTFD) at concentration of 10-5 g/ml was added in the medium, the arrest was not seen for more than 30 min. 3. Arrhythmic contractions induced by the electrical square wave stimulation of threshold intensity were prevented by TTFD at 10-4 g/ml which was added to the medium. 4. These effects of TTFD at respective concentrations were seen even 30 min after the drug was removed from the medium, when the atria had been pre-incubated with the drug for one hour before the removal. 5. From these results, it was assumed that TTFD might show these effects against the extrinsic physical invasions through the stabilization of the tissue membrane.", "PMID": 956923}
{"id": "pubmed23n0032_2483", "title": "Relationship between serum vitamin E levels and serum cholesterol in normal adults in rural regions of Yamagata Prefecture, Japan.", "content": "Serum vitamin E and cholesterol levels were determined for the residents in rural regions of Yamagata Prefecture, Japan. The average serum vitamin E were 0.99 +/- 0.25 mg/100ml in 166 men, and 1.06 +/- 0.26 mg/100ml in 178 women. Only 0.6% of the subjects were below 0.5 mg/100 ml. There was a significant difference between the serum vitamin E levels of men and women. However there were no statistically significant differences among serum vitamin E of various age groups. The best correlation for serum vitamin E was found with serum cholesterol.", "contents": "Relationship between serum vitamin E levels and serum cholesterol in normal adults in rural regions of Yamagata Prefecture, Japan. Serum vitamin E and cholesterol levels were determined for the residents in rural regions of Yamagata Prefecture, Japan. The average serum vitamin E were 0.99 +/- 0.25 mg/100ml in 166 men, and 1.06 +/- 0.26 mg/100ml in 178 women. Only 0.6% of the subjects were below 0.5 mg/100 ml. There was a significant difference between the serum vitamin E levels of men and women. However there were no statistically significant differences among serum vitamin E of various age groups. The best correlation for serum vitamin E was found with serum cholesterol.", "PMID": 956925}
{"id": "pubmed23n0032_2484", "title": "Effect of vitamin B6 deficiency on collagen metabolism in rats.", "content": "The effects of VB6 deficiency on collagen metabolism were studied using young and adult rats. It was observed in VB6 deficient rats, that both the amount of urinary hydroxyproline and salt soluble fraction of skin collagen decreased. Furthermore, decrease of the alpha-component and increase of the beta-component were also found in the soluble collagen. However, the activity of plasma amine oxidase was lower and the aldehyde content in the acid soluble collagen were also lower than the control group. These changes were revealed more clearly in young growing rats than in adult rats. Based on these results, it may be that VB6 participates in the allysine (alpha-amino-adipic-delta-semialdehyde) formation which is considered as the first step of collagen maturation and also in the synthesis of protocollagen peptide chains.", "contents": "Effect of vitamin B6 deficiency on collagen metabolism in rats. The effects of VB6 deficiency on collagen metabolism were studied using young and adult rats. It was observed in VB6 deficient rats, that both the amount of urinary hydroxyproline and salt soluble fraction of skin collagen decreased. Furthermore, decrease of the alpha-component and increase of the beta-component were also found in the soluble collagen. However, the activity of plasma amine oxidase was lower and the aldehyde content in the acid soluble collagen were also lower than the control group. These changes were revealed more clearly in young growing rats than in adult rats. Based on these results, it may be that VB6 participates in the allysine (alpha-amino-adipic-delta-semialdehyde) formation which is considered as the first step of collagen maturation and also in the synthesis of protocollagen peptide chains.", "PMID": 956926}
{"id": "pubmed23n0032_2485", "title": "Effects of riboflavin deficiency on the lipids of rat liver.", "content": "Newly weaned male rats were maintained on a riboflavin-free diet for 5 weeks, and a study was made on the effect of the deficiency upon liver lipids. The content of glyceride in the livers varied among the deficient rats. High contents of glyceride were demonstrated in one-third of the deficient rats, whereas the similar level as that of control was shown in the remaining deficient rats. Contents of phospholipids and relative amounts of individual phospholipids were not altered significantly by the deficiency. Riboflavin deficiency exerted effects on fatty acid components of liver lipids. The composition of fatty acids of triglycerides varied in the deficient rats depending on the content of glycerides. However trends of increase in linoleic acid and decrease in palmitic acid towards fatty liver were observed in the deficiency in comparison with the control. On the other hand, the changes in phospholipid fatty acids were similar in all deficient rats, and the increase in linoleic acid and the decrease in arachidonic acid were brought about by the deficiency compared with controls, respectively. In liver homogenates, the incorporation of 14C-palmitate into triglycerides was higher in the deficient rats irrespective of the presence or absence of fluorde but incorporation into phospholipids was slightly lower in the deficient rats than in control animals.", "contents": "Effects of riboflavin deficiency on the lipids of rat liver. Newly weaned male rats were maintained on a riboflavin-free diet for 5 weeks, and a study was made on the effect of the deficiency upon liver lipids. The content of glyceride in the livers varied among the deficient rats. High contents of glyceride were demonstrated in one-third of the deficient rats, whereas the similar level as that of control was shown in the remaining deficient rats. Contents of phospholipids and relative amounts of individual phospholipids were not altered significantly by the deficiency. Riboflavin deficiency exerted effects on fatty acid components of liver lipids. The composition of fatty acids of triglycerides varied in the deficient rats depending on the content of glycerides. However trends of increase in linoleic acid and decrease in palmitic acid towards fatty liver were observed in the deficiency in comparison with the control. On the other hand, the changes in phospholipid fatty acids were similar in all deficient rats, and the increase in linoleic acid and the decrease in arachidonic acid were brought about by the deficiency compared with controls, respectively. In liver homogenates, the incorporation of 14C-palmitate into triglycerides was higher in the deficient rats irrespective of the presence or absence of fluorde but incorporation into phospholipids was slightly lower in the deficient rats than in control animals.", "PMID": 956927}
{"id": "pubmed23n0032_2486", "title": "Intestinal absorption of beta-alanine, anserine and carnosine in rats.", "content": "Absorption beta-alanine, anserine or carnosine from rat intestine was studied in vivo by a force feeding method and in vitro using an everted sac method. Possibility of anserine and carnosine hydrolysis prior to intestinal absorption was also investigated using a glycylleucine dipeptidase-containing fraction prepared from rat intestine. The following results were obtained. 1) Anserine and carnosine were absorbed as they were from rat small intestine. 2) Both anserine and carnosine were partially hydrolyzed in vitro by the glycylleucine dipeptidase-containing fraction. Carnosine was hydrolyzed faster than anserine. The above rather conflicting results suggest that physiological amounts of anserine and carnosine might be absorbed from rat small intestine in dipeptide forms.", "contents": "Intestinal absorption of beta-alanine, anserine and carnosine in rats. Absorption beta-alanine, anserine or carnosine from rat intestine was studied in vivo by a force feeding method and in vitro using an everted sac method. Possibility of anserine and carnosine hydrolysis prior to intestinal absorption was also investigated using a glycylleucine dipeptidase-containing fraction prepared from rat intestine. The following results were obtained. 1) Anserine and carnosine were absorbed as they were from rat small intestine. 2) Both anserine and carnosine were partially hydrolyzed in vitro by the glycylleucine dipeptidase-containing fraction. Carnosine was hydrolyzed faster than anserine. The above rather conflicting results suggest that physiological amounts of anserine and carnosine might be absorbed from rat small intestine in dipeptide forms.", "PMID": 956928}
{"id": "pubmed23n0032_2487", "title": "Alanine metabolism in pyridoxine-depleted rat liver.", "content": "Alanine metabolism in normal and pyridoxine-deficient rats was studied in vivo and in vitro. Incorporation of 14C-alanine into various liver components was determined and no difference was shown between normal and deficient animals in the incorporation into liver homogenates, lipid, protein and plasma glucose. Using the liver slice syste, gluconeogenic activity from alanine or pyruvate was 40% lower in deficient rats compared with the activity of normal rats. However, inhibition was completely removed by the addition of 2-oxoglutarate to alanine. Penicillamine did not affect glucose formation alanine in the liver slice.", "contents": "Alanine metabolism in pyridoxine-depleted rat liver. Alanine metabolism in normal and pyridoxine-deficient rats was studied in vivo and in vitro. Incorporation of 14C-alanine into various liver components was determined and no difference was shown between normal and deficient animals in the incorporation into liver homogenates, lipid, protein and plasma glucose. Using the liver slice syste, gluconeogenic activity from alanine or pyruvate was 40% lower in deficient rats compared with the activity of normal rats. However, inhibition was completely removed by the addition of 2-oxoglutarate to alanine. Penicillamine did not affect glucose formation alanine in the liver slice.", "PMID": 956929}
{"id": "pubmed23n0032_2488", "title": "Effects of carnitine administration, fasting, and exercise on urinary carnitine excretion in man.", "content": "1. In this study, the carnitine level in 24-hr urine has been determined in males, (a) before and after a single dose of 500 mg (as carnitine) of DL-carnitine chloride, (b) during fasting, and (c) before and after a severe running program (3 x 2,000 m/8-8.5 min). 2. After the administration of DL-carnitine chloride, the urinary carnitine excretion was increased by only approximately 10% of the dose, suggesting a large body pool size of carnitine. 3. Urinary carnitine excretion was significantly increased during five-day fasting; a maximum level of 2.17 +/- 0.24 (mean and SD) mmoles/day was 4.6 times higher than the usual level of 0.47 +/- 0.10 mmoles/day (p less than 0.001). Respiratory quotients (RQ) decreased significantly (p less than 0.01) from the control value of 0.81 +/- 0.03 to the value of 0.75 +/- .02 after five-day fasting and was significantly correlated with urinary carnitine levels (r = 0.62, p less than 0.05). 4. The urinary excretion of carnitine increased slightly with heavy running exercise. 5. The results are discussed in relation to the physiologic regulation of the rate of carnitine synthesis.", "contents": "Effects of carnitine administration, fasting, and exercise on urinary carnitine excretion in man. 1. In this study, the carnitine level in 24-hr urine has been determined in males, (a) before and after a single dose of 500 mg (as carnitine) of DL-carnitine chloride, (b) during fasting, and (c) before and after a severe running program (3 x 2,000 m/8-8.5 min). 2. After the administration of DL-carnitine chloride, the urinary carnitine excretion was increased by only approximately 10% of the dose, suggesting a large body pool size of carnitine. 3. Urinary carnitine excretion was significantly increased during five-day fasting; a maximum level of 2.17 +/- 0.24 (mean and SD) mmoles/day was 4.6 times higher than the usual level of 0.47 +/- 0.10 mmoles/day (p less than 0.001). Respiratory quotients (RQ) decreased significantly (p less than 0.01) from the control value of 0.81 +/- 0.03 to the value of 0.75 +/- .02 after five-day fasting and was significantly correlated with urinary carnitine levels (r = 0.62, p less than 0.05). 4. The urinary excretion of carnitine increased slightly with heavy running exercise. 5. The results are discussed in relation to the physiologic regulation of the rate of carnitine synthesis.", "PMID": 956930}
{"id": "pubmed23n0032_2489", "title": "Ultracytochemical studies on the effects of thiamine and thiamine tetrahydrofurfuryl disulfide on acetylcholinesterase activity in guinea pig atria.", "content": "As a result of histological and ultracytochemical investigations concerning the influence of thiamine and its disulfide derivative, thiamine tetrahydrofurfuryl disulfide (TTFD), on acetylcholinesterase (AChE) in extirpated guinea pig atria, it was found that there was a clear increase in the AChE activity by 10(-4) M TTFD, a weak increase with 10(-5) M and an extremely weak increase, about the same as that in only the nutrient (Locke's solution), with 5 x 10(-4) M. However, with 10(-4) M of thiamine there was a tendency for the activity to be the same degree as with Lock's solution alone or be even inhibited at times.", "contents": "Ultracytochemical studies on the effects of thiamine and thiamine tetrahydrofurfuryl disulfide on acetylcholinesterase activity in guinea pig atria. As a result of histological and ultracytochemical investigations concerning the influence of thiamine and its disulfide derivative, thiamine tetrahydrofurfuryl disulfide (TTFD), on acetylcholinesterase (AChE) in extirpated guinea pig atria, it was found that there was a clear increase in the AChE activity by 10(-4) M TTFD, a weak increase with 10(-5) M and an extremely weak increase, about the same as that in only the nutrient (Locke's solution), with 5 x 10(-4) M. However, with 10(-4) M of thiamine there was a tendency for the activity to be the same degree as with Lock's solution alone or be even inhibited at times.", "PMID": 956931}
{"id": "pubmed23n0032_2490", "title": "In vivo incorporation of glucose carbon into brain and liver proteins in biotin-deficient rats.", "content": "The in vivo utilization of D[U-14C]glucose with particular reference to its incorporation into brain and liver proteins was studied in biotin-deficient rats. The data show a significant reduction in the incorporation of glucose carbon into brain and liver proteins. A disturbance in the intermediary metabolism of glucose to be one of the factors responsible for the decreased incorporation of the label into proteins in biotin deficiency.", "contents": "In vivo incorporation of glucose carbon into brain and liver proteins in biotin-deficient rats. The in vivo utilization of D[U-14C]glucose with particular reference to its incorporation into brain and liver proteins was studied in biotin-deficient rats. The data show a significant reduction in the incorporation of glucose carbon into brain and liver proteins. A disturbance in the intermediary metabolism of glucose to be one of the factors responsible for the decreased incorporation of the label into proteins in biotin deficiency.", "PMID": 956932}
{"id": "pubmed23n0032_2491", "title": "Legal aspects of medical confidentiality in the occupational setting.", "content": "The law of confidentiality of medical information in the occupational setting is undeveloped and subject to change. Nevertheless, when a physician-patient relationship is established, all of the traditional trappings, including the duty to maintain confidences, go along with it. In other settings, such as preemployment physicals, examinees implicitly consent for their \"fitness' for the job to be disclosed, but do not assume that specific findings will be disclosed without their informed and written consent. To promote physician automony and worker confidence in the occupational physician, an explicit policy of confidentiality should be developed and agreed to by both management and workers. This policy should spell out the conditions under which specific information will be made available to the employer and should guarantee the confidentiality of all other medical information. Workers should also be given routine access to their medical files, and offered the chance to refute and have amended information in them that they can demonstrate is inaccurate. In short, workers in the occupational setting should, in general, be treated like private patients. Such a policy will satisfy both the law and the ethical standards of physicians, and will promote worker trust in the occupational physician.", "contents": "Legal aspects of medical confidentiality in the occupational setting. The law of confidentiality of medical information in the occupational setting is undeveloped and subject to change. Nevertheless, when a physician-patient relationship is established, all of the traditional trappings, including the duty to maintain confidences, go along with it. In other settings, such as preemployment physicals, examinees implicitly consent for their \"fitness' for the job to be disclosed, but do not assume that specific findings will be disclosed without their informed and written consent. To promote physician automony and worker confidence in the occupational physician, an explicit policy of confidentiality should be developed and agreed to by both management and workers. This policy should spell out the conditions under which specific information will be made available to the employer and should guarantee the confidentiality of all other medical information. Workers should also be given routine access to their medical files, and offered the chance to refute and have amended information in them that they can demonstrate is inaccurate. In short, workers in the occupational setting should, in general, be treated like private patients. Such a policy will satisfy both the law and the ethical standards of physicians, and will promote worker trust in the occupational physician.", "PMID": 956937}
{"id": "pubmed23n0032_2492", "title": "Long-term mortality study of steelworkers.", "content": "This study examines the cause-specific mortality of men employed as masons in the steel industry. Their mortality experience is compared with the mortality predicted by the age--and race--specific death rates for a control group consisting of those steelworkers whose first job in 1953 was not in the mason department. The relative risks of dying from selected causes were highly dependent on race. The most important observations are: (1) The nonwhites have an overall excess risk when compared to the nonmason group. This excess is not confined to any particular category and appears to be due to the cumulative effect of an excess in many different categories. (2) The white masons have an excess risk for nonmalignant respiratory disease. Furthermore, this risk increases with length of exposure. (3) The whites have an excess risk for respiratory cancer when compared to the nonmason group. This excess is not present in the later years of the follow-up period. (4) The interpretation of the findings for cardiovascular disease remain unclear. Results for cerebral vascular disease and hypertensive disease in nonwhites are suggestive, but need further investigation before any strong statements can be made.", "contents": "Long-term mortality study of steelworkers. This study examines the cause-specific mortality of men employed as masons in the steel industry. Their mortality experience is compared with the mortality predicted by the age--and race--specific death rates for a control group consisting of those steelworkers whose first job in 1953 was not in the mason department. The relative risks of dying from selected causes were highly dependent on race. The most important observations are: (1) The nonwhites have an overall excess risk when compared to the nonmason group. This excess is not confined to any particular category and appears to be due to the cumulative effect of an excess in many different categories. (2) The white masons have an excess risk for nonmalignant respiratory disease. Furthermore, this risk increases with length of exposure. (3) The whites have an excess risk for respiratory cancer when compared to the nonmason group. This excess is not present in the later years of the follow-up period. (4) The interpretation of the findings for cardiovascular disease remain unclear. Results for cerebral vascular disease and hypertensive disease in nonwhites are suggestive, but need further investigation before any strong statements can be made.", "PMID": 956938}
{"id": "pubmed23n0032_2493", "title": "Variations of pulmonary function amongst workers in cotton mills.", "content": "Lung function studies and clinical examinations of 493 workers in eight cotton mills in New South Wales revealed 12 workers with byssinosis. The reasons for the low incidence of byssinosis in view of relatively high cotton dust in air concentrations are discussed briefly.", "contents": "Variations of pulmonary function amongst workers in cotton mills. Lung function studies and clinical examinations of 493 workers in eight cotton mills in New South Wales revealed 12 workers with byssinosis. The reasons for the low incidence of byssinosis in view of relatively high cotton dust in air concentrations are discussed briefly.", "PMID": 956940}
{"id": "pubmed23n0032_2494", "title": "Experimental allergic sialoadenitis. VIII. Acute parotitis following intraductal instillation of antiserum to salivary antigens in the rat.", "content": "Antiserum to ammonium sulphate-soluble proteins of rat saliva was raised in rabbits. The introduction of this antiserum, via the excretory duct, into the parotid gland of rats induced severe acute sialoadentis. It is suggested that the inflammatory response developed as a result of formation of immune complexes, composed of acinar epithelial antigens and the antibodies introduced from without.", "contents": "Experimental allergic sialoadenitis. VIII. Acute parotitis following intraductal instillation of antiserum to salivary antigens in the rat. Antiserum to ammonium sulphate-soluble proteins of rat saliva was raised in rabbits. The introduction of this antiserum, via the excretory duct, into the parotid gland of rats induced severe acute sialoadentis. It is suggested that the inflammatory response developed as a result of formation of immune complexes, composed of acinar epithelial antigens and the antibodies introduced from without.", "PMID": 956953}
{"id": "pubmed23n0032_2495", "title": "The ultrastructure of benign pigmented naevi and melanocarcinomas in man.", "content": "Nine benign pigmented naevi and 16 melanocarcinomas (malignant melanomas) were studied by light and transmission electron microscopy and the fine structure of benign and malignant melanocytes is described. It was found that malignant melanocytes generally exhibit a range of abnormalities of structure of nuclei and cytoplasmic organelles which distinguish them clearly from their benign counterparts. Of the abnormalities, those affecting the specific organelle of the melanocyte, the melanosome, were the most constantly present. No firm evidence was found for the existence of two types of melanosome or two types of melanocarcinoma. Although electron microscopy is capable of showing in great detail within individual melanocytes many changes which are clearly associated with malignancy, the technique does not appear to offer significant advantages over light microscopy in determining the diagnosis and prognosis in this group of conditions.", "contents": "The ultrastructure of benign pigmented naevi and melanocarcinomas in man. Nine benign pigmented naevi and 16 melanocarcinomas (malignant melanomas) were studied by light and transmission electron microscopy and the fine structure of benign and malignant melanocytes is described. It was found that malignant melanocytes generally exhibit a range of abnormalities of structure of nuclei and cytoplasmic organelles which distinguish them clearly from their benign counterparts. Of the abnormalities, those affecting the specific organelle of the melanocyte, the melanosome, were the most constantly present. No firm evidence was found for the existence of two types of melanosome or two types of melanocarcinoma. Although electron microscopy is capable of showing in great detail within individual melanocytes many changes which are clearly associated with malignancy, the technique does not appear to offer significant advantages over light microscopy in determining the diagnosis and prognosis in this group of conditions.", "PMID": 956954}
{"id": "pubmed23n0032_2496", "title": "Atrophy and apoptosis in the cyclical human endometrium.", "content": "Electron microscopy of human cyclical endometrium indicates that there is considerable loss of gland epithelial cells with formation of apoptotic bodies in the late secretory, premenstrual and menstrual phases, but also to a lesser extent in the proliferative phase. Apoptotic bodies containing nuclear fragments may be recognised on light microscopy as basophilic or Feulgen positive granules. These granules accumulate in greatest numbers towards the end of the cycle and may be used as an additional aid in dating curettings.", "contents": "Atrophy and apoptosis in the cyclical human endometrium. Electron microscopy of human cyclical endometrium indicates that there is considerable loss of gland epithelial cells with formation of apoptotic bodies in the late secretory, premenstrual and menstrual phases, but also to a lesser extent in the proliferative phase. Apoptotic bodies containing nuclear fragments may be recognised on light microscopy as basophilic or Feulgen positive granules. These granules accumulate in greatest numbers towards the end of the cycle and may be used as an additional aid in dating curettings.", "PMID": 956955}
{"id": "pubmed23n0032_2497", "title": "The state of knee meniscal fibrocartilage in Liverpool necropsies.", "content": "The state of the fibrocartilaginous menisci of the adult knee joint has been studied at necropsies in the city of Liverpool on a random series of 94 white Europeans (55 men, 39 women) aged 21 to 94 yr. The left lateral meniscus was selected for particular attention; in 20 of the subjects the left medial meniscus was also examined. The gross and histological appearance of the superior and inferior surfaces of the lateral meniscus and of its free inner edge is described and illustrated at various ages. The findings from necropsy specimens indicate that the fibrocartilage of the adult meniscus is susceptible to a focal process of matrix fraying and splitting which has several features similar to those seen in fibrillation of hyaline articular cartilage. Certain differences in detail between meniscal and hyaline cartilage in the pattern of cell changes at sites of overt splitting are, however, apparent. The implications of the findings are discussed in terms of an hypothesis that cartilage fibrillation represents a disruption of the collagen framework, and that \"wear\", of the matrix due to mechanical factors plays a major part in its development.", "contents": "The state of knee meniscal fibrocartilage in Liverpool necropsies. The state of the fibrocartilaginous menisci of the adult knee joint has been studied at necropsies in the city of Liverpool on a random series of 94 white Europeans (55 men, 39 women) aged 21 to 94 yr. The left lateral meniscus was selected for particular attention; in 20 of the subjects the left medial meniscus was also examined. The gross and histological appearance of the superior and inferior surfaces of the lateral meniscus and of its free inner edge is described and illustrated at various ages. The findings from necropsy specimens indicate that the fibrocartilage of the adult meniscus is susceptible to a focal process of matrix fraying and splitting which has several features similar to those seen in fibrillation of hyaline articular cartilage. Certain differences in detail between meniscal and hyaline cartilage in the pattern of cell changes at sites of overt splitting are, however, apparent. The implications of the findings are discussed in terms of an hypothesis that cartilage fibrillation represents a disruption of the collagen framework, and that \"wear\", of the matrix due to mechanical factors plays a major part in its development.", "PMID": 956956}
{"id": "pubmed23n0032_2498", "title": "Some effects of phenacetin on the rat.", "content": "Rats aged from 2 to 4 mth were given phenacetin 100 mg/kg/day and 1-14 g/kg/day, orally, for up to 26 1/2 wk and 3 wk, respectively. Signs of premature ageing such as senile plagues, neurofibrillary tangles and lipofucsin pigment were not demonstrated in the brains of these animals.", "contents": "Some effects of phenacetin on the rat. Rats aged from 2 to 4 mth were given phenacetin 100 mg/kg/day and 1-14 g/kg/day, orally, for up to 26 1/2 wk and 3 wk, respectively. Signs of premature ageing such as senile plagues, neurofibrillary tangles and lipofucsin pigment were not demonstrated in the brains of these animals.", "PMID": 956957}
{"id": "pubmed23n0032_2499", "title": "Systemic lupus erythematosus in childhood correlations between changes in disease activity and serum complement levels.", "content": "Serial complement component (C3 and C4) determinations were performed in 26 children with systemic lupus erythematosus. Twenty-one children with SLE had 52 episodes of C3 depression (mean duration 25 weeks); only 11 of these children had active nephritis when serum concentrations of complement were depressed. Fourteen children had active rash associated with low C3; in seven of these children rash was the only clinical evidence of disease activity. Ten children had active CNS disease; in seven children the CNS involvement correlated with low C3. In general, variations in serum concentrations of C4 did not reflect changes in SLE activity which were not reflected by changes in serum concentrations of C3. Serum C4 occasionally remained depressed longer than C3, perhaps reflecting continuing subclinical disease activity. Increased C3 occurred in 18 of 26 children as doses of corticosteroid were increased, in six of 14 when cyclophosphamide was added, and in two children when hydroxychloroquine was added. Our findings suggest that a wide variety of manifestations of childhood SLE may produce hypocomplementemia. In addition to renal disease, variations in serum concentrations of C3 and C4 can reflect, or occasionally predict, changes in rash and CNS disease.", "contents": "Systemic lupus erythematosus in childhood correlations between changes in disease activity and serum complement levels. Serial complement component (C3 and C4) determinations were performed in 26 children with systemic lupus erythematosus. Twenty-one children with SLE had 52 episodes of C3 depression (mean duration 25 weeks); only 11 of these children had active nephritis when serum concentrations of complement were depressed. Fourteen children had active rash associated with low C3; in seven of these children rash was the only clinical evidence of disease activity. Ten children had active CNS disease; in seven children the CNS involvement correlated with low C3. In general, variations in serum concentrations of C4 did not reflect changes in SLE activity which were not reflected by changes in serum concentrations of C3. Serum C4 occasionally remained depressed longer than C3, perhaps reflecting continuing subclinical disease activity. Increased C3 occurred in 18 of 26 children as doses of corticosteroid were increased, in six of 14 when cyclophosphamide was added, and in two children when hydroxychloroquine was added. Our findings suggest that a wide variety of manifestations of childhood SLE may produce hypocomplementemia. In addition to renal disease, variations in serum concentrations of C3 and C4 can reflect, or occasionally predict, changes in rash and CNS disease.", "PMID": 956959}
{"id": "pubmed23n0032_2500", "title": "Nephritis in systemic lupus erythematosus in children.", "content": "Twenty-five patients, aged six to 18 years, with lupus nephritis, followed for two to 103 months (median 22 months), have been classified according to renal histology into groups with diffuse proliferative glomerulonephritis (18), focal proliferative nephritis (2), and membranous nephropathy (5). Correlations have been made among specific histologic groups, clinical findings, renal function studies, urinary findings, and response to therapy. In sequential renal biopsies, lesions progressed in most patients with diffuse proliferative and membranous changes; however, chronic renal failure occurred in only one patient and the five-year survival rate (60.9%) is better than previously reported in pediatric patients.", "contents": "Nephritis in systemic lupus erythematosus in children. Twenty-five patients, aged six to 18 years, with lupus nephritis, followed for two to 103 months (median 22 months), have been classified according to renal histology into groups with diffuse proliferative glomerulonephritis (18), focal proliferative nephritis (2), and membranous nephropathy (5). Correlations have been made among specific histologic groups, clinical findings, renal function studies, urinary findings, and response to therapy. In sequential renal biopsies, lesions progressed in most patients with diffuse proliferative and membranous changes; however, chronic renal failure occurred in only one patient and the five-year survival rate (60.9%) is better than previously reported in pediatric patients.", "PMID": 956960}
{"id": "pubmed23n0032_2501", "title": "Distal renal tubular acidosis in children with chronic hydronephrosis.", "content": "The difference between the PCO2 of alkalinized urine and the PCO2 of blood has been proposed as an indirect means of assessing distal nephron H+ secretion. Following oral bicarbonate loading and alkalinization of the urine to pH greater than or equal to 7.2, eight of nine children with chronic hydronephrosis and four children with idiopathic distal RTA had low (U-B)PCO2 values when compared with seven normal subjects. This findings leads us to conclude that chronic hydronephrosis may cause a form of distal RTA which persists many years after surgical diversion procedures have been carried out.", "contents": "Distal renal tubular acidosis in children with chronic hydronephrosis. The difference between the PCO2 of alkalinized urine and the PCO2 of blood has been proposed as an indirect means of assessing distal nephron H+ secretion. Following oral bicarbonate loading and alkalinization of the urine to pH greater than or equal to 7.2, eight of nine children with chronic hydronephrosis and four children with idiopathic distal RTA had low (U-B)PCO2 values when compared with seven normal subjects. This findings leads us to conclude that chronic hydronephrosis may cause a form of distal RTA which persists many years after surgical diversion procedures have been carried out.", "PMID": 956961}
{"id": "pubmed23n0032_2502", "title": "Severe combined immunodeficiency with leukopenia (reticular dysgenesis) in siblings: immunologic and histopathologic findings.", "content": "The hematologic and histologic features of two, nontwin, male siblings with severe combined immunodeficiency and variable granulocytopenia are compared to the four previously reported cases of reticular dysgenesis. These sibs died at 50 and 3 days of age, respectively, with Pseudomonas sepsis and congenital cytomegalovirus infection, respectively. A maternal uncle has selective IgA deficiency. Cord blood from the second sib contained a normal percentage of E-rosetting lymphocytes; however, these lymphocytes failed to respond to mitogenic stimulation in vitro. Erythrocyte and lymphocyte levels of adenosine deaminase were elevated in the father and the second sib. Serum immunoglobulin concentrations were low in both siblings.", "contents": "Severe combined immunodeficiency with leukopenia (reticular dysgenesis) in siblings: immunologic and histopathologic findings. The hematologic and histologic features of two, nontwin, male siblings with severe combined immunodeficiency and variable granulocytopenia are compared to the four previously reported cases of reticular dysgenesis. These sibs died at 50 and 3 days of age, respectively, with Pseudomonas sepsis and congenital cytomegalovirus infection, respectively. A maternal uncle has selective IgA deficiency. Cord blood from the second sib contained a normal percentage of E-rosetting lymphocytes; however, these lymphocytes failed to respond to mitogenic stimulation in vitro. Erythrocyte and lymphocyte levels of adenosine deaminase were elevated in the father and the second sib. Serum immunoglobulin concentrations were low in both siblings.", "PMID": 956962}
{"id": "pubmed23n0032_2503", "title": "Neutrophil oxidative metabolism in sickle cell disease.", "content": "Bacterial infections are the most frequent cause of death and an important factor of morbidity in sickle cell disease. A defect in oxidative metabolism of neutrophils from these patients has been reported as a possible cause for these infections. Since normal neutrophil functions are essential in the defense against bacteria, it seemed important to reassess the capacity of neutrophils from patients with sickle cell disease to undergo the metabolic events associated with phagocytic bactericidal activity. Accordingly, neutrophils from patients and controls were compared for their ability to reduce nitroblue tetrazolium dye, to activate the hexose monophosphate shunt, and to generate superoxide anion, hydrogen peroxide, and chemiluminescence. Patients' cells performed normally in each of these assays and, in addition, killed Staphylococcus aureus as well as did cells from controls. Thus, an abnormality of neutrophil oxidative metabolism cannot explain the propensity to bacterial infections in sickle cell disease.", "contents": "Neutrophil oxidative metabolism in sickle cell disease. Bacterial infections are the most frequent cause of death and an important factor of morbidity in sickle cell disease. A defect in oxidative metabolism of neutrophils from these patients has been reported as a possible cause for these infections. Since normal neutrophil functions are essential in the defense against bacteria, it seemed important to reassess the capacity of neutrophils from patients with sickle cell disease to undergo the metabolic events associated with phagocytic bactericidal activity. Accordingly, neutrophils from patients and controls were compared for their ability to reduce nitroblue tetrazolium dye, to activate the hexose monophosphate shunt, and to generate superoxide anion, hydrogen peroxide, and chemiluminescence. Patients' cells performed normally in each of these assays and, in addition, killed Staphylococcus aureus as well as did cells from controls. Thus, an abnormality of neutrophil oxidative metabolism cannot explain the propensity to bacterial infections in sickle cell disease.", "PMID": 956963}
{"id": "pubmed23n0032_2504", "title": "Course of cystic fibrosis in 95 patients.", "content": "The course of 95 patients with cystic fibrosis is presented. Survivors have a mean follow-up period of over 14 years (minimum: 13 years). Of 45 patients diagnosed prior to extensive irreversible pulmonary involvement, only one has died and none is disabled. In contrast, of the other 50 patients diagnosed after substantial irreversible pulmonary disease was present, 26 have died. Mortality and morbidity has been greater in females. Possible factors contributing to the improving prognosis include early diagnosis, aggressive management with comprehensive care, easy access to specialized care, and improved antimicrobial therapy.", "contents": "Course of cystic fibrosis in 95 patients. The course of 95 patients with cystic fibrosis is presented. Survivors have a mean follow-up period of over 14 years (minimum: 13 years). Of 45 patients diagnosed prior to extensive irreversible pulmonary involvement, only one has died and none is disabled. In contrast, of the other 50 patients diagnosed after substantial irreversible pulmonary disease was present, 26 have died. Mortality and morbidity has been greater in females. Possible factors contributing to the improving prognosis include early diagnosis, aggressive management with comprehensive care, easy access to specialized care, and improved antimicrobial therapy.", "PMID": 956964}
{"id": "pubmed23n0032_2505", "title": "Course of cystic fibrosis in black patients.", "content": "Seventeen black patients showed typical sweat gland, gastrointestinal, and pulmonary manifestations of cystic fibrosis. There was an unusually high incidence of meconium ileus equivalent (35%). Three infant siblings of patients in this study had died of cystic fibrosis prior to referral of a family member to this center. One patient died at age four years after emotional problems interfered with the treatment program; status asthmaticus played a major role in his terminal respiratory failure. The remainder of the patients have had substantially less morbidity from pulmonary disease than a comparable, much larger group of white patients. Only one patient, age 28, who is married and employed full-time has severe pulmonary involvement as assessed by pulmonary function studies and chest roentgenogram. The 16 surviving patients have a mean age of 13 1/2 years after a mean follow-up period of 10 years. If black patients with cystic fibrosis survive infancy, they then, as a group, may have a relatively good prognosis.", "contents": "Course of cystic fibrosis in black patients. Seventeen black patients showed typical sweat gland, gastrointestinal, and pulmonary manifestations of cystic fibrosis. There was an unusually high incidence of meconium ileus equivalent (35%). Three infant siblings of patients in this study had died of cystic fibrosis prior to referral of a family member to this center. One patient died at age four years after emotional problems interfered with the treatment program; status asthmaticus played a major role in his terminal respiratory failure. The remainder of the patients have had substantially less morbidity from pulmonary disease than a comparable, much larger group of white patients. Only one patient, age 28, who is married and employed full-time has severe pulmonary involvement as assessed by pulmonary function studies and chest roentgenogram. The 16 surviving patients have a mean age of 13 1/2 years after a mean follow-up period of 10 years. If black patients with cystic fibrosis survive infancy, they then, as a group, may have a relatively good prognosis.", "PMID": 956965}
{"id": "pubmed23n0032_2506", "title": "Mucosal recovery in treated childhood celiac disease (gluten-sensitive enteropathy).", "content": "Follow-up studies on 36 children, in whom celiac disease (gluten-sensitive enteropathy) was established by gluten challenge, were carried out after management on gluten-free diets for a mean of six years. Evaluations included measurement of height and weight, which for the group approximated normal distributions, and histologic examination of the duodenal or jejunal mucosa. Mucosal morphology was  regarded as normal in 16, and there were minimal changes in 20. Epithelial cell height was within the  normal range in all the children. Interepithelial lymphocytes were within normal range in the majority and lymphoid cells in the lamina propria were not different from those in control subjects. Mucosal lactase was significantly lower in patients than in control subjects in the duodenum and the jejunum, whereas sucrase and alkaline phosphatase values were significantly lower in the jejunum but not in the duodenum. Low content of mucosal lactase and increased numbers of interepithelial lymphocytes may be sensitive indicators of persisting ingestion of gluten in mucosa that is otherwise normal or approximately so in appearance.", "contents": "Mucosal recovery in treated childhood celiac disease (gluten-sensitive enteropathy). Follow-up studies on 36 children, in whom celiac disease (gluten-sensitive enteropathy) was established by gluten challenge, were carried out after management on gluten-free diets for a mean of six years. Evaluations included measurement of height and weight, which for the group approximated normal distributions, and histologic examination of the duodenal or jejunal mucosa. Mucosal morphology was  regarded as normal in 16, and there were minimal changes in 20. Epithelial cell height was within the  normal range in all the children. Interepithelial lymphocytes were within normal range in the majority and lymphoid cells in the lamina propria were not different from those in control subjects. Mucosal lactase was significantly lower in patients than in control subjects in the duodenum and the jejunum, whereas sucrase and alkaline phosphatase values were significantly lower in the jejunum but not in the duodenum. Low content of mucosal lactase and increased numbers of interepithelial lymphocytes may be sensitive indicators of persisting ingestion of gluten in mucosa that is otherwise normal or approximately so in appearance.", "PMID": 956966}
{"id": "pubmed23n0032_2507", "title": "Complex alpha-thalassemia-like syndrome: a cause of neonatal normoblastemia.", "content": "A previously unrecognized hypochromic anemia associated  with marked normoblastemia during the newborn period is reported. One male and two female siblings and a first cousin had a hypochromic anemia and marked normoblastemia (300 to 900 normoblast index per 100 white blood cells) at birth. Globin chain synthesis studies on peripheral blood of the proband at birth indicated the presence of alpha-thalassemia trait with possible reduced gamma chain synthesis. Studies of globin chain synthesis on the father, two older affected siblings of the proband, and the proband at 1.5 years of age revealed alpha-thalassemia trait. The data suggest this complex alpha-thalassemia-like condition as a new syndrome associated with marked neonatal normoblastemia.", "contents": "Complex alpha-thalassemia-like syndrome: a cause of neonatal normoblastemia. A previously unrecognized hypochromic anemia associated  with marked normoblastemia during the newborn period is reported. One male and two female siblings and a first cousin had a hypochromic anemia and marked normoblastemia (300 to 900 normoblast index per 100 white blood cells) at birth. Globin chain synthesis studies on peripheral blood of the proband at birth indicated the presence of alpha-thalassemia trait with possible reduced gamma chain synthesis. Studies of globin chain synthesis on the father, two older affected siblings of the proband, and the proband at 1.5 years of age revealed alpha-thalassemia trait. The data suggest this complex alpha-thalassemia-like condition as a new syndrome associated with marked neonatal normoblastemia.", "PMID": 956973}
{"id": "pubmed23n0032_2508", "title": "Neonatal pneumatosis coli: a mild form of neonatal necrotizing enterocolitis.", "content": "A distinctive form of necrotizing enterocolitis was observed in seven newborn  infants in a 4 1/2-year period. Characteristically these patients had gross blood in the stools, but other local and systemic signs were mild or absent. Radiography mainly revealed submucosal intramural intestinal gas which was limited to the colon (pneumatosis coli). There was no evidence of small bowel involvement as judged by the absence of small intestinal pneumatosis or distension. This form of colonic disease should be recognized as a benign variety of neonatal necrotizing enterocolitis that carries a favorable prognosis and responds to medical management without sequelae.", "contents": "Neonatal pneumatosis coli: a mild form of neonatal necrotizing enterocolitis. A distinctive form of necrotizing enterocolitis was observed in seven newborn  infants in a 4 1/2-year period. Characteristically these patients had gross blood in the stools, but other local and systemic signs were mild or absent. Radiography mainly revealed submucosal intramural intestinal gas which was limited to the colon (pneumatosis coli). There was no evidence of small bowel involvement as judged by the absence of small intestinal pneumatosis or distension. This form of colonic disease should be recognized as a benign variety of neonatal necrotizing enterocolitis that carries a favorable prognosis and responds to medical management without sequelae.", "PMID": 956974}
{"id": "pubmed23n0032_2509", "title": "Necrotizing enterocolitis in term infants.", "content": "Despite the fact that necrotizing enterocolitis is considered a disease of premature infants, 20% of all affected infants at Babies Hospital over the past 20 years were products of term gestations. Two distinct subgroups of such infants were noted (1) five infants with congenital heart disease and/or congestive heart failure (e.g.hypoplastic left heart syndrome), all but one of whom developed the disease in the first week of life; (2) eight infants who developed the disease at a much later age after a protracted period of diarrhea. This histopathologic features of the disease in term infants are the same as those in premature infants. Further, the pathogenesis of the disease in term infants does not appear to differ basically from that in premature infants. These facts, lead away from the concept of NEC as a disease of simple etiology.", "contents": "Necrotizing enterocolitis in term infants. Despite the fact that necrotizing enterocolitis is considered a disease of premature infants, 20% of all affected infants at Babies Hospital over the past 20 years were products of term gestations. Two distinct subgroups of such infants were noted (1) five infants with congenital heart disease and/or congestive heart failure (e.g.hypoplastic left heart syndrome), all but one of whom developed the disease in the first week of life; (2) eight infants who developed the disease at a much later age after a protracted period of diarrhea. This histopathologic features of the disease in term infants are the same as those in premature infants. Further, the pathogenesis of the disease in term infants does not appear to differ basically from that in premature infants. These facts, lead away from the concept of NEC as a disease of simple etiology.", "PMID": 956975}
{"id": "pubmed23n0032_2510", "title": "The effect of paraben preservatives on albumin binding of bilirubin.", "content": "The interaction of methylparaben and propylparaben with bilirubin-albumin complexes was studied using difference spectra, Sephadex gel filtration, red blood cell uptake of bilirubin, and the peroxidase assay. Methylparaben was found to be a weak competitor with bilirubin for binding to primary albumin-binding sites but a strong binding competitor (similar to sulfisoxazole) at secondary sites. The displacing effects of methylparaben and sulfisoxazole were additive. Propylparaben bound to albumin but did not displace bilirubin. Drugs and injectable saline and water preparations which contain methylparaben should be avoided in jaundiced newborn infants when the high-affinity albumin-binding sites approach saturation.", "contents": "The effect of paraben preservatives on albumin binding of bilirubin. The interaction of methylparaben and propylparaben with bilirubin-albumin complexes was studied using difference spectra, Sephadex gel filtration, red blood cell uptake of bilirubin, and the peroxidase assay. Methylparaben was found to be a weak competitor with bilirubin for binding to primary albumin-binding sites but a strong binding competitor (similar to sulfisoxazole) at secondary sites. The displacing effects of methylparaben and sulfisoxazole were additive. Propylparaben bound to albumin but did not displace bilirubin. Drugs and injectable saline and water preparations which contain methylparaben should be avoided in jaundiced newborn infants when the high-affinity albumin-binding sites approach saturation.", "PMID": 956978}
{"id": "pubmed23n0032_2511", "title": "Growth, behavioral, and psychologic measurements of adopted children: the influences of genetic and socioeconomic factors in a prospective study.", "content": "Perinatal, growth, social, and psychologic data, collected through seven years of age on 144 adopted children and on 288 matched controls as part of a prospective study of 3,120 children, were subjected to statistical analysis. Only single births and healthy children were included. The adopted subjects were  divided into two groups: 94 children who were adopted by nonrelatives (77 prior to one year of age and 17 after one year of age); and 50 children who remained with their biologic mothers and were subsequently adopted by stepfathers. Children who were adopted prior to one year of age had significantly higher IQs at four years of age then did their controls; at seven years of age, however, their IQs were similar even though the adopted children were raised in a higher socioeconomic environment. The adopted children had better academic achievement and were taller at four and seven years of age than were the controls. The performance of the children adopted after one year of age, even though they were raised in a higher socioeconomic environment, were, were like those of their controls at both four and seven years  of age. The performance of the children who remained with their biologic mothers was similar to that of their controls. Maternal intelligence generally did not correlate well with adopted children's intelligence; however, a specific significant positive correlation could be shown between maternal intelligence and the intelligence of children who remained with their biologic mothers.", "contents": "Growth, behavioral, and psychologic measurements of adopted children: the influences of genetic and socioeconomic factors in a prospective study. Perinatal, growth, social, and psychologic data, collected through seven years of age on 144 adopted children and on 288 matched controls as part of a prospective study of 3,120 children, were subjected to statistical analysis. Only single births and healthy children were included. The adopted subjects were  divided into two groups: 94 children who were adopted by nonrelatives (77 prior to one year of age and 17 after one year of age); and 50 children who remained with their biologic mothers and were subsequently adopted by stepfathers. Children who were adopted prior to one year of age had significantly higher IQs at four years of age then did their controls; at seven years of age, however, their IQs were similar even though the adopted children were raised in a higher socioeconomic environment. The adopted children had better academic achievement and were taller at four and seven years of age than were the controls. The performance of the children adopted after one year of age, even though they were raised in a higher socioeconomic environment, were, were like those of their controls at both four and seven years  of age. The performance of the children who remained with their biologic mothers was similar to that of their controls. Maternal intelligence generally did not correlate well with adopted children's intelligence; however, a specific significant positive correlation could be shown between maternal intelligence and the intelligence of children who remained with their biologic mothers.", "PMID": 956980}
{"id": "pubmed23n0032_2512", "title": "Thyroid function in neonatal hypothyroidism.", "content": "Various aspects of the thyroid function have been measured in 28 cases of neonatal hypothyroidism detected by means of the Quebec Screening Program for Metabolic Diseases. In all instances the T4 value in the blood of filter paper spot was below 2 SD of the mean of the day, averaging 0.39 +/- 0.04 ng/40 mul (mean +/- SEM) of eluted blood. The T4 value of a second similar sample averaged 0.22 +/- 0.04 ng/mul of eluted blood; this value was significantly lower than the first one. The serum T4 concentration was decreased in all the infants, whereas three of them had a normal serum TSH concentration. At least three groups of patients could be identified: (1) patients with primary thyroid failure, (2) those with secondary or tertiary hypothyrodism, and (3) those with abnormal synthesis of thyroid hormone.", "contents": "Thyroid function in neonatal hypothyroidism. Various aspects of the thyroid function have been measured in 28 cases of neonatal hypothyroidism detected by means of the Quebec Screening Program for Metabolic Diseases. In all instances the T4 value in the blood of filter paper spot was below 2 SD of the mean of the day, averaging 0.39 +/- 0.04 ng/40 mul (mean +/- SEM) of eluted blood. The T4 value of a second similar sample averaged 0.22 +/- 0.04 ng/mul of eluted blood; this value was significantly lower than the first one. The serum T4 concentration was decreased in all the infants, whereas three of them had a normal serum TSH concentration. At least three groups of patients could be identified: (1) patients with primary thyroid failure, (2) those with secondary or tertiary hypothyrodism, and (3) those with abnormal synthesis of thyroid hormone.", "PMID": 956995}
{"id": "pubmed23n0032_2513", "title": "TSH measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism.", "content": "A sensitive radioimmunoassay for the measurement of TSH in the eluate of blood spotted on filter paper has been developed. The method is consistently sensitive to 0.1 to 0.25 muU of TSH and enables the detection of values equivalent to 6 to 15 muU/ml of serum. The measurement of TSH in the filter paper spot in all infants with low filter paper spot T4 has permitted rapid confirmation of 10 cases of neonatal hypothyroidism. However, cases of hypothalamic hypothyroidism with low or normal filter paper spot TSH concentrations would have been missed using only this method. Since these patients represent approximately 10% of our neonatal hypothyroid population, we do not recommend this method as a primary screening procedure, but rather as a confirmatory test which will accelerate the diagnosis and therefore the onset of therapy.", "contents": "TSH measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism. A sensitive radioimmunoassay for the measurement of TSH in the eluate of blood spotted on filter paper has been developed. The method is consistently sensitive to 0.1 to 0.25 muU of TSH and enables the detection of values equivalent to 6 to 15 muU/ml of serum. The measurement of TSH in the filter paper spot in all infants with low filter paper spot T4 has permitted rapid confirmation of 10 cases of neonatal hypothyroidism. However, cases of hypothalamic hypothyroidism with low or normal filter paper spot TSH concentrations would have been missed using only this method. Since these patients represent approximately 10% of our neonatal hypothyroid population, we do not recommend this method as a primary screening procedure, but rather as a confirmatory test which will accelerate the diagnosis and therefore the onset of therapy.", "PMID": 956996}
{"id": "pubmed23n0032_2514", "title": "Delayed onset of hypopituitarism: sequelae of therapeutic irradiation of central nervous system, eye, and middle ear tumors.", "content": "Four children with short stature who received irradiation to the head in conventional doses had clinical and laboratory evidence of hypothalamic-pituitary hormone deficiencies several years later. Growth hormone was deficient in all. One patient also had evidence of TSH, ACTH, and gonadotropin deficiency. Basal prolactin levels and prolactin response to synthetic TRF were normal in all patients tested. Treatment with human growth hormone significantly increased growth rate. We suggest that children should have the hypothalamic-pituitary area shielded from irradiation. Periodic measurements of hypothalamic-pituitary function should be performed in children who have had irradiation to the head, in order to detect and treat hormonal deficiencies before growth and development are seriously compromised.", "contents": "Delayed onset of hypopituitarism: sequelae of therapeutic irradiation of central nervous system, eye, and middle ear tumors. Four children with short stature who received irradiation to the head in conventional doses had clinical and laboratory evidence of hypothalamic-pituitary hormone deficiencies several years later. Growth hormone was deficient in all. One patient also had evidence of TSH, ACTH, and gonadotropin deficiency. Basal prolactin levels and prolactin response to synthetic TRF were normal in all patients tested. Treatment with human growth hormone significantly increased growth rate. We suggest that children should have the hypothalamic-pituitary area shielded from irradiation. Periodic measurements of hypothalamic-pituitary function should be performed in children who have had irradiation to the head, in order to detect and treat hormonal deficiencies before growth and development are seriously compromised.", "PMID": 956997}
{"id": "pubmed23n0032_2515", "title": "Juvenile diabetes mellitus, optic atrophy, sensory nerve deafness, and diabetes insipidus--a syndrome.", "content": "Four patients with diabetes mellitus, optic atrophy, and high-frequency neurosensory hearing loss, two of whom also had diabetes insipidus, are described. The frequency of this syndrome among patients with juvenile diabetes appears to be between 1/148 and 1/175. Because of the progressive nature of the disabilities and the autosomal recessive mode of inheritance, careful monitoring of all juvenile diabetic patients for other signs of the syndrome is warranted.", "contents": "Juvenile diabetes mellitus, optic atrophy, sensory nerve deafness, and diabetes insipidus--a syndrome. Four patients with diabetes mellitus, optic atrophy, and high-frequency neurosensory hearing loss, two of whom also had diabetes insipidus, are described. The frequency of this syndrome among patients with juvenile diabetes appears to be between 1/148 and 1/175. Because of the progressive nature of the disabilities and the autosomal recessive mode of inheritance, careful monitoring of all juvenile diabetic patients for other signs of the syndrome is warranted.", "PMID": 956998}
{"id": "pubmed23n0032_2516", "title": "Developmental change in red blood cell volume: implication in screening infants and children for iron deficiency and thalassemia trait.", "content": "The mean corpuscular volumen when determined by electronic counter is an accurate tool for identification of children with microcytosis due to either iron deficiency or thalassemia trait. The purpose of this report is to describe the normal developmental changes in MCV that occur in children afler 6 months of age. In 211 healthy infants and children screened to exclude those with borderline or overt iron deficiency, thalassemia trait, or hemoglobinopathy, we found that the lower limit of normal for MCV is 70 ft between 10 and 17 months of age and that there is a gradual increase of MCV with age; the lower limit is 74 between 1 1/2 and 4 years and 76 between 4 and 7 years. All of these values are well below the minimum adult level of 80 fl.", "contents": "Developmental change in red blood cell volume: implication in screening infants and children for iron deficiency and thalassemia trait. The mean corpuscular volumen when determined by electronic counter is an accurate tool for identification of children with microcytosis due to either iron deficiency or thalassemia trait. The purpose of this report is to describe the normal developmental changes in MCV that occur in children afler 6 months of age. In 211 healthy infants and children screened to exclude those with borderline or overt iron deficiency, thalassemia trait, or hemoglobinopathy, we found that the lower limit of normal for MCV is 70 ft between 10 and 17 months of age and that there is a gradual increase of MCV with age; the lower limit is 74 between 1 1/2 and 4 years and 76 between 4 and 7 years. All of these values are well below the minimum adult level of 80 fl.", "PMID": 956999}
{"id": "pubmed23n0032_2517", "title": "Percutaneous cardiac catheterization and atrial balloon septostomy in pediatrics.", "content": "Between November, 1971, and July, 1975, 688 patients ranging in age from six hours to 21 years have undergone percutaneous transfemoral venous and/or arterial catheterization at the University of Oregon Health Sciences Center. This represents 93% of all venous catheterizations of the right side of the heart and 97% of all arterial catheterizations of the left side of the heart in the pediatric age range. One hundred and ninety-five patients (29% of the group) weighed 5 kg or less and 133 (20% of the group) were neonates. There were virtually no complications following the venous studies. The complication rate following percutaneous arterial studies is lower than is the reported experience with cut-down arteriotomies. Long-term survival following percutaneous atrial balloon septostomy in D-transposition of the great arteries was comparable to that following cut-down balloon septostomy. The ease of accomplishment, significantly lower complication rate, and successful accomplishment of atrial balloon septostomy prompt us to advocate that the percutaneous technique of cardiac catheterization be extended to include the neonate and young infant who weighsless than 5 kg.", "contents": "Percutaneous cardiac catheterization and atrial balloon septostomy in pediatrics. Between November, 1971, and July, 1975, 688 patients ranging in age from six hours to 21 years have undergone percutaneous transfemoral venous and/or arterial catheterization at the University of Oregon Health Sciences Center. This represents 93% of all venous catheterizations of the right side of the heart and 97% of all arterial catheterizations of the left side of the heart in the pediatric age range. One hundred and ninety-five patients (29% of the group) weighed 5 kg or less and 133 (20% of the group) were neonates. There were virtually no complications following the venous studies. The complication rate following percutaneous arterial studies is lower than is the reported experience with cut-down arteriotomies. Long-term survival following percutaneous atrial balloon septostomy in D-transposition of the great arteries was comparable to that following cut-down balloon septostomy. The ease of accomplishment, significantly lower complication rate, and successful accomplishment of atrial balloon septostomy prompt us to advocate that the percutaneous technique of cardiac catheterization be extended to include the neonate and young infant who weighsless than 5 kg.", "PMID": 957000}
{"id": "pubmed23n0032_2518", "title": "Tracheal aspirate lecithin/sphingomyelin ratios as predictors of recovery from respiratory distress syndrome.", "content": "In thirty infants with severe respiratory distress syndrome who required endotracheal intubation, serial tracheal aspirate lecithin/sphingomyelin ratios were determined. There was 19 survivors and 11 nonsurvivors. The L/S ratios were plotted as a function of postnatal age; the ratios of the survivors demonstrated a significant increase between 24 and 96 hours. Nonsurvivors did not show this change. There was a significant difference on Days 3, 4, and 5 between survivors and nonsurvivors in the percentage of L/S ratios greater than 2.5. Daily mean L/S ratios correctly identified the outcome of 23 of 24 infants alive on Days 4 and/or 5 and were a more accurate prediction of survival than a single L/S ratio. The changes in the L/S ratios of the tracheal aspirates were not related to gestational age.", "contents": "Tracheal aspirate lecithin/sphingomyelin ratios as predictors of recovery from respiratory distress syndrome. In thirty infants with severe respiratory distress syndrome who required endotracheal intubation, serial tracheal aspirate lecithin/sphingomyelin ratios were determined. There was 19 survivors and 11 nonsurvivors. The L/S ratios were plotted as a function of postnatal age; the ratios of the survivors demonstrated a significant increase between 24 and 96 hours. Nonsurvivors did not show this change. There was a significant difference on Days 3, 4, and 5 between survivors and nonsurvivors in the percentage of L/S ratios greater than 2.5. Daily mean L/S ratios correctly identified the outcome of 23 of 24 infants alive on Days 4 and/or 5 and were a more accurate prediction of survival than a single L/S ratio. The changes in the L/S ratios of the tracheal aspirates were not related to gestational age.", "PMID": 957008}
{"id": "pubmed23n0032_2519", "title": "Disturbances of the transitional circulation: spectrum of pulmonary hypertension and myocardial dysfunction.", "content": "Thirteen neonates presented with central cyanosis due to right-to-left shunting across the foramen ovale or the ductus arteriosus or both. In three infants shunting occurred secondary to pulmonary vascular obstruction, presumably related to pulmonary vasospasm (persistence of the fetal or transitional circulation). In ten neonates right-to-left shunting was associated with heart failure; seven of these neonates had systemic hypotension.", "contents": "Disturbances of the transitional circulation: spectrum of pulmonary hypertension and myocardial dysfunction. Thirteen neonates presented with central cyanosis due to right-to-left shunting across the foramen ovale or the ductus arteriosus or both. In three infants shunting occurred secondary to pulmonary vascular obstruction, presumably related to pulmonary vasospasm (persistence of the fetal or transitional circulation). In ten neonates right-to-left shunting was associated with heart failure; seven of these neonates had systemic hypotension.", "PMID": 957009}
{"id": "pubmed23n0032_2520", "title": "Socioeconomic factors in relation to fetal growth in white infants.", "content": "Over 1,200 white mothers who were delivered consecutively at this medical center were classified in four different socioeconomic classes according to family affluence and occupations of the heat of the household. The frequency of low-birth-weight infants was highest in the lowest socioeconomic class. The high incidence of LBW infants in the lowest socioeconiomic group was not affected by any significant increase in number of mothers with medical problems or medical complications of pregnancy; it was dependent on the large number of mothers who were involved in four specific practices, largely of their own choosing, including low-weight gains, cigarette smoking, use of certain drugs during pregnancy, and refraining from all prenatal care. Mothers in the four socioeconomic classes who were not involved with these four specific practices (smoking, etc.) and whose pregnancies were free of medical problems and complications had uniformly low incidences of LBW infants and the mean birth weights of their infants were uniformly high and not significantly different. Unfavorable outcome of pregnancy with respect to fetal growth in this study appeared to depend less directly on socioeconomic circumstances than on the four specific maternal practices listed above.", "contents": "Socioeconomic factors in relation to fetal growth in white infants. Over 1,200 white mothers who were delivered consecutively at this medical center were classified in four different socioeconomic classes according to family affluence and occupations of the heat of the household. The frequency of low-birth-weight infants was highest in the lowest socioeconomic class. The high incidence of LBW infants in the lowest socioeconiomic group was not affected by any significant increase in number of mothers with medical problems or medical complications of pregnancy; it was dependent on the large number of mothers who were involved in four specific practices, largely of their own choosing, including low-weight gains, cigarette smoking, use of certain drugs during pregnancy, and refraining from all prenatal care. Mothers in the four socioeconomic classes who were not involved with these four specific practices (smoking, etc.) and whose pregnancies were free of medical problems and complications had uniformly low incidences of LBW infants and the mean birth weights of their infants were uniformly high and not significantly different. Unfavorable outcome of pregnancy with respect to fetal growth in this study appeared to depend less directly on socioeconomic circumstances than on the four specific maternal practices listed above.", "PMID": 957011}
{"id": "pubmed23n0032_2521", "title": "The effects of four commonly used drugs on platelet function.", "content": "A number of commonly used drugs have been reportd to inter", "contents": "The effects of four commonly used drugs on platelet function. A number of commonly used drugs have been reportd to inter", "PMID": 957014}
{"id": "pubmed23n0032_2522", "title": "The effect of maternally administered drugs on bilirubin concentrations in the newborn infant.", "content": "The effects of drugs administered to pregnant women on bilirubin concentrations in 1,107 consecutively born infants are presented. Administration of narcotic agents, barbiturates, aspirin, chloral hydrate, reserpine, and phenytoin sodium all resulted in lowering of infant serum bilirubin concentrations. Diazepam and, to a lesser extent, oxytocin caused an elevation of infant serum bilirubin concentrations. Although many drugs were shown to alter serum bilirubin levels significantly, the clinical importance of such alterations was not dramatic except possibly in special circumstances. The phenothiazine derivatives, general or local anesthesia, sulfadimidine, ampicillin, and penicillin had no such effect on the newborn infant when given to the mother before delivery.", "contents": "The effect of maternally administered drugs on bilirubin concentrations in the newborn infant. The effects of drugs administered to pregnant women on bilirubin concentrations in 1,107 consecutively born infants are presented. Administration of narcotic agents, barbiturates, aspirin, chloral hydrate, reserpine, and phenytoin sodium all resulted in lowering of infant serum bilirubin concentrations. Diazepam and, to a lesser extent, oxytocin caused an elevation of infant serum bilirubin concentrations. Although many drugs were shown to alter serum bilirubin levels significantly, the clinical importance of such alterations was not dramatic except possibly in special circumstances. The phenothiazine derivatives, general or local anesthesia, sulfadimidine, ampicillin, and penicillin had no such effect on the newborn infant when given to the mother before delivery.", "PMID": 957015}
{"id": "pubmed23n0032_2523", "title": "Risks to the offspring of women treated with hydantoin anticonvulsants, with emphasis on the fetal hydantoin syndrome.", "content": "The fetal hydantoin syndrome is a variable pattern of altered growth and performance which includes unusual facies, distal phalangeal hyoplasia, and other defects occurring in some infants exposed in utero to hydantoins. A prospective study of 35 infants exposed prenatally to this class of anticonvulsants showed that 11% had sufficient features to be classified as having the fetal hydantoin syndrome. An additional 31% displayed some features compatible with the prenatal effects of hydantoins. A case-control study of 104 infants whose mothers received hydantoins during pregnancy supports these conclusions. Reduction of intellectual ability in infants with the fetal hydantoin syndrome is the area of greatest concern. Women being treated with hydantoin anticonvulsants should be told of the nature and magnitude of risks to the developing fetus before considering a pregnancy.", "contents": "Risks to the offspring of women treated with hydantoin anticonvulsants, with emphasis on the fetal hydantoin syndrome. The fetal hydantoin syndrome is a variable pattern of altered growth and performance which includes unusual facies, distal phalangeal hyoplasia, and other defects occurring in some infants exposed in utero to hydantoins. A prospective study of 35 infants exposed prenatally to this class of anticonvulsants showed that 11% had sufficient features to be classified as having the fetal hydantoin syndrome. An additional 31% displayed some features compatible with the prenatal effects of hydantoins. A case-control study of 104 infants whose mothers received hydantoins during pregnancy supports these conclusions. Reduction of intellectual ability in infants with the fetal hydantoin syndrome is the area of greatest concern. Women being treated with hydantoin anticonvulsants should be told of the nature and magnitude of risks to the developing fetus before considering a pregnancy.", "PMID": 957016}
{"id": "pubmed23n0032_2524", "title": "Intellectual and social development following prolonged isolation.", "content": "A patient with severe combined immunodeficiency required protective isolation for the first year of life. He was discharged after an effective treatment program was developed and has remained well. Developmental testing at 14 and 16 months revealed near age level social and intellectual functioning. Optimal nursing care, frequent and prolonged maternal contact, and the patient's adaptive capacities permitted rapid adjustment to normal life following prolonged isolation.", "contents": "Intellectual and social development following prolonged isolation. A patient with severe combined immunodeficiency required protective isolation for the first year of life. He was discharged after an effective treatment program was developed and has remained well. Developmental testing at 14 and 16 months revealed near age level social and intellectual functioning. Optimal nursing care, frequent and prolonged maternal contact, and the patient's adaptive capacities permitted rapid adjustment to normal life following prolonged isolation.", "PMID": 957018}
{"id": "pubmed23n0032_2525", "title": "Differentiation of Herpetomonas megaseliae: effects of hydroxyurea on morphology and growth.", "content": "Herpetomonas megaseliae grown in the presence of hydroxyurea showed a decline in population during the first 24 hr, eventually increasing slightly over the 7-day incubation. Throughtout the 7-day incubation period the population of the treated organisms never equaled that of the control organisms. The percentage of promastigotes, as opposed to para-and opisthomastigotes, in the treated cultures did not differ from that in the control cultures through the first 3 days in incubation. Thereafter, the percentage of promastigotes in the treated cultures dropped significantly below that of the controls. At the light microscope level, the treated organisms showed an increase in size with associated striations at the anterior end. A multiflagellate (nondividing) condition was also observed in the hydroxyurea-treated organisms. TEM and SEM studies showed pellicular convolutions, which were interpreted as the striations observed under the light microscope. The differnces in population number, kinetoplast number and position and pellicular morphology between control and experimental cultures suggests that enhanced differentiation and abortive cytokinesis are the net effects of hydroxyurea on Herpetomonas megaseliae.", "contents": "Differentiation of Herpetomonas megaseliae: effects of hydroxyurea on morphology and growth. Herpetomonas megaseliae grown in the presence of hydroxyurea showed a decline in population during the first 24 hr, eventually increasing slightly over the 7-day incubation. Throughtout the 7-day incubation period the population of the treated organisms never equaled that of the control organisms. The percentage of promastigotes, as opposed to para-and opisthomastigotes, in the treated cultures did not differ from that in the control cultures through the first 3 days in incubation. Thereafter, the percentage of promastigotes in the treated cultures dropped significantly below that of the controls. At the light microscope level, the treated organisms showed an increase in size with associated striations at the anterior end. A multiflagellate (nondividing) condition was also observed in the hydroxyurea-treated organisms. TEM and SEM studies showed pellicular convolutions, which were interpreted as the striations observed under the light microscope. The differnces in population number, kinetoplast number and position and pellicular morphology between control and experimental cultures suggests that enhanced differentiation and abortive cytokinesis are the net effects of hydroxyurea on Herpetomonas megaseliae.", "PMID": 957031}
{"id": "pubmed23n0032_2526", "title": "Plasmodium (Giovannolaia) pedioecetii from gallinaceous birds of Colorado.", "content": "Forty-nine Colorado birds of 6 galliform species were positive for Plasmodium (Giovannolaia) pedioecetii which we feel to be the same parasite described by Wetmore (1939) from a shaptailed grouse from North Dakota. Except for its presence in Darwin's tinamou (Nothura darwinii) from Colorado, it has been reported only from gallinaceous birds. Most stages were predominantly subpolar to polar. There were a daily 8 am to noon peak of merozoite production, but no synchronicity. Merozoite number ranged from 8 to 22, with a mean of 10.9. Gametocytes were long and slender, some curving around one end of the host nucleus.", "contents": "Plasmodium (Giovannolaia) pedioecetii from gallinaceous birds of Colorado. Forty-nine Colorado birds of 6 galliform species were positive for Plasmodium (Giovannolaia) pedioecetii which we feel to be the same parasite described by Wetmore (1939) from a shaptailed grouse from North Dakota. Except for its presence in Darwin's tinamou (Nothura darwinii) from Colorado, it has been reported only from gallinaceous birds. Most stages were predominantly subpolar to polar. There were a daily 8 am to noon peak of merozoite production, but no synchronicity. Merozoite number ranged from 8 to 22, with a mean of 10.9. Gametocytes were long and slender, some curving around one end of the host nucleus.", "PMID": 957032}
{"id": "pubmed23n0032_2527", "title": "Parabothriocephalus sagitticeps (Sleggs 1927) comb. n. (Cestoda: Parabothriocephalidae) from Sebastes paucispinis of southern and central California.", "content": "Parabothriocephalus sagitticeps (Sleggs 1927) from Sebastes paucispinis is redescribed and given a generic reassignment. The new combination is based on the dorsal, submarginal genital attrium and tests arrangement. The tapeworm prevalence was 84% of 354 rockfish in the coastal waters of Southern and Central California.", "contents": "Parabothriocephalus sagitticeps (Sleggs 1927) comb. n. (Cestoda: Parabothriocephalidae) from Sebastes paucispinis of southern and central California. Parabothriocephalus sagitticeps (Sleggs 1927) from Sebastes paucispinis is redescribed and given a generic reassignment. The new combination is based on the dorsal, submarginal genital attrium and tests arrangement. The tapeworm prevalence was 84% of 354 rockfish in the coastal waters of Southern and Central California.", "PMID": 957033}
{"id": "pubmed23n0032_2528", "title": "Immunological response to experimental echnococcosis in Meriones unguiculatus.", "content": "Varying doses of protoscolices of Echinococcus granulosus were injected intraperitoneally into Mongolian jirds, Swiss mice, and golden hamsters. After 4 months the animals were killed and the numbers of developing cysts counted. Jirds were by far the most susceptible hosts. Doses of 500 protoscolices produced 100% infection rates in both weanlings and adults. Comparable infection rates in weanling mice required inocula of 5,000 protoscolices, but even at this dose hamsters were refractory to infection. Three of 5 jirds developed pulmonary cysts after intravenous administration of 500 protoscolices. Serologic responses in infected jirds were followed using the indirect hemagglutination tests and a purified fraction of hydatid cyst fluid (HCF) as antigen. Titers reached a plateau after 15 weeks and were maintained for several months. Much brisker serologic responses occurred in animals sensitized with the antigenic fraction in various adjuvant vehicles. At no time during infection did jirds show any evidence of immediate hypersensitivity to HCF in direct skin tests, passive cutaneous anaphylactic tests, or after intravenous challenge with antigen. After artificial immunization with hapten-conjugated antigens or HCF, jirds underwent a fatal shock following intravenous challenge with antigen. HCF provoked the appearance of circulating antibodies which were capable of sensitizing normal recipients for passive systemic anaphylaxis after a latent period of 72 hr. It is concluded that the failure of the infected  jird to develop immediate hypersensitivity responses to E. granulosus represents a marked deviation from the parrern of the immune response in echinococcosis in man and domestic animals and must be considered in the future use of this animal in experimental studies on the host-parasite relationship.", "contents": "Immunological response to experimental echnococcosis in Meriones unguiculatus. Varying doses of protoscolices of Echinococcus granulosus were injected intraperitoneally into Mongolian jirds, Swiss mice, and golden hamsters. After 4 months the animals were killed and the numbers of developing cysts counted. Jirds were by far the most susceptible hosts. Doses of 500 protoscolices produced 100% infection rates in both weanlings and adults. Comparable infection rates in weanling mice required inocula of 5,000 protoscolices, but even at this dose hamsters were refractory to infection. Three of 5 jirds developed pulmonary cysts after intravenous administration of 500 protoscolices. Serologic responses in infected jirds were followed using the indirect hemagglutination tests and a purified fraction of hydatid cyst fluid (HCF) as antigen. Titers reached a plateau after 15 weeks and were maintained for several months. Much brisker serologic responses occurred in animals sensitized with the antigenic fraction in various adjuvant vehicles. At no time during infection did jirds show any evidence of immediate hypersensitivity to HCF in direct skin tests, passive cutaneous anaphylactic tests, or after intravenous challenge with antigen. After artificial immunization with hapten-conjugated antigens or HCF, jirds underwent a fatal shock following intravenous challenge with antigen. HCF provoked the appearance of circulating antibodies which were capable of sensitizing normal recipients for passive systemic anaphylaxis after a latent period of 72 hr. It is concluded that the failure of the infected  jird to develop immediate hypersensitivity responses to E. granulosus represents a marked deviation from the parrern of the immune response in echinococcosis in man and domestic animals and must be considered in the future use of this animal in experimental studies on the host-parasite relationship.", "PMID": 957034}
{"id": "pubmed23n0032_2529", "title": "Eucyathostomum webbi sp. n. (Strongyloidea: Cloacinidae) from white-tailed deer (Odocoileus virginianus).", "content": "The new species is described from white-tailed deer of McIntosh County, Georgia, and Pushmataha County, Oklahoma. Eucyathostomum webbi sp. n. differs from E. longesubulatum (type) in possessing more internal leaf crown elements, unbarbed spiculte tips, variability in the configuration of the dorsal ray of the male bursa, and shorter vagina and ovejectors; from E. copulatum in being much larger and not having males and females permanently united; from E. spinulosum in the origin of the externo-dorsal ray of the male bursa; and from E. dentatum in having more internal leaf crown elements, shorter spicules, a distinct cephalic vesicle, no dorsal gutter, and a pectinated flange posterior to the vulva. Problems in the taxonomy of the genus Eucyathostomum are discussed. Specimens previously identified as E. longesubulatum from southeastern white-tailed deer were reexamined and found to be conspecific with E. webbi.", "contents": "Eucyathostomum webbi sp. n. (Strongyloidea: Cloacinidae) from white-tailed deer (Odocoileus virginianus). The new species is described from white-tailed deer of McIntosh County, Georgia, and Pushmataha County, Oklahoma. Eucyathostomum webbi sp. n. differs from E. longesubulatum (type) in possessing more internal leaf crown elements, unbarbed spiculte tips, variability in the configuration of the dorsal ray of the male bursa, and shorter vagina and ovejectors; from E. copulatum in being much larger and not having males and females permanently united; from E. spinulosum in the origin of the externo-dorsal ray of the male bursa; and from E. dentatum in having more internal leaf crown elements, shorter spicules, a distinct cephalic vesicle, no dorsal gutter, and a pectinated flange posterior to the vulva. Problems in the taxonomy of the genus Eucyathostomum are discussed. Specimens previously identified as E. longesubulatum from southeastern white-tailed deer were reexamined and found to be conspecific with E. webbi.", "PMID": 957035}
{"id": "pubmed23n0032_2530", "title": "Post-labial sensory structures on the cecal worm, Heterakis gallinarum.", "content": "Sense organs that lie just posterior to the lips on the sides of the body of Heterakis gallinarum have been examined by scanning and transmission electron microscopy. The sense organs appear externally as a short peg surrounded by a collarlike elevation of cuticle. Each sense organ contains the endings of 2 sensory dendrites; 1 extending to the tip of the peg is probably chemosensitive, while the other ending just within the cuticular cylinder that projects internally from the peg, is probably mechanosensitive.", "contents": "Post-labial sensory structures on the cecal worm, Heterakis gallinarum. Sense organs that lie just posterior to the lips on the sides of the body of Heterakis gallinarum have been examined by scanning and transmission electron microscopy. The sense organs appear externally as a short peg surrounded by a collarlike elevation of cuticle. Each sense organ contains the endings of 2 sensory dendrites; 1 extending to the tip of the peg is probably chemosensitive, while the other ending just within the cuticular cylinder that projects internally from the peg, is probably mechanosensitive.", "PMID": 957036}
{"id": "pubmed23n0032_2531", "title": "A simple method for collecting infective larvae of Litomosoides carinii.", "content": "A method is described whereby substantial quantities of infective larvae of Litomosoides carinii can be obtained with relative ease. In each of 2 separate experiments, over 5,000 infective larvae were recovered by soaking the pelts of 5 or 7 Mongolian jirds (Meriones unguiculatus) that had been exposed for 6 or 7 hr to infected tropical rat mites (Ornithonyssus bacoti). The mites had been infected either 7 to 8 or 9 to 10 days earlier by exposure to jirds with patent infections. Ninety-four percent of the total number of infective larvae recovered and migrated out of the pelts within 2 1/2 hr. Jirds inoculated subcutaneously with infective larvae collected in this manner exhibited high mean percent recoveries at necropsy, i.e. 79 and 60 at 5 and 29 days postinoculation, respectively. In addition, the ranges in worm recoveries were narrow.", "contents": "A simple method for collecting infective larvae of Litomosoides carinii. A method is described whereby substantial quantities of infective larvae of Litomosoides carinii can be obtained with relative ease. In each of 2 separate experiments, over 5,000 infective larvae were recovered by soaking the pelts of 5 or 7 Mongolian jirds (Meriones unguiculatus) that had been exposed for 6 or 7 hr to infected tropical rat mites (Ornithonyssus bacoti). The mites had been infected either 7 to 8 or 9 to 10 days earlier by exposure to jirds with patent infections. Ninety-four percent of the total number of infective larvae recovered and migrated out of the pelts within 2 1/2 hr. Jirds inoculated subcutaneously with infective larvae collected in this manner exhibited high mean percent recoveries at necropsy, i.e. 79 and 60 at 5 and 29 days postinoculation, respectively. In addition, the ranges in worm recoveries were narrow.", "PMID": 957037}
{"id": "pubmed23n0032_2532", "title": "Cruorifilaria tuberocauda gen. et sp. n. (Nematoda: Filarioidea) from the capybara, Hydrochoerus hydrochaeris in colombia.", "content": "Cruorifilaria tuberocauda gen. et sp. n. is described from blood vessels of the kidney, heart, and lungs of the capybara, Hyrochoerus hydrochaeris, in Colombia, S.A. The adult worms are robust, of moderate size (females approximately 42 mm long, males about 27 mm long), and tapered at both ends. The males have unequal, dissimilar spicules, lack a gubernaculum and possess both pre- and postanal papillae. The unsheathed microfilaria is found in the peripheral blood. Even though a severe tissue response is seen in the walls of the blood vessels occupied by the adult worms, it appears to be a common parasite in the population of capybaras surveyed.", "contents": "Cruorifilaria tuberocauda gen. et sp. n. (Nematoda: Filarioidea) from the capybara, Hydrochoerus hydrochaeris in colombia. Cruorifilaria tuberocauda gen. et sp. n. is described from blood vessels of the kidney, heart, and lungs of the capybara, Hyrochoerus hydrochaeris, in Colombia, S.A. The adult worms are robust, of moderate size (females approximately 42 mm long, males about 27 mm long), and tapered at both ends. The males have unequal, dissimilar spicules, lack a gubernaculum and possess both pre- and postanal papillae. The unsheathed microfilaria is found in the peripheral blood. Even though a severe tissue response is seen in the walls of the blood vessels occupied by the adult worms, it appears to be a common parasite in the population of capybaras surveyed.", "PMID": 957038}
{"id": "pubmed23n0032_2533", "title": "Studies on resistance in snails. 7. Evidence of interference with the defense reaction in Biomphalaria glabrata by trematode larvae.", "content": "Echinostoma lindoense sporocysts that develop from irradiated miracidia normally are destroyed by amebocyte capsules in the ventricle of Biomphalaria glabrata within 10 days postexposure. The survival period of these ventricular sporocysts was considerably longer in snails that also harbored normal sporocysts of E. lindoense, Paryphostomum segregatum, or Schistosoma mansoni. Protection of irradiated E. lindoense sporocysts by the same of different trematode species is presumed to be the result of an active process by which normal sporocysts interfere with capsule formation and protect themselves and other trematode larvae from encapsulation. Homologous protection was stronger than heterologous.", "contents": "Studies on resistance in snails. 7. Evidence of interference with the defense reaction in Biomphalaria glabrata by trematode larvae. Echinostoma lindoense sporocysts that develop from irradiated miracidia normally are destroyed by amebocyte capsules in the ventricle of Biomphalaria glabrata within 10 days postexposure. The survival period of these ventricular sporocysts was considerably longer in snails that also harbored normal sporocysts of E. lindoense, Paryphostomum segregatum, or Schistosoma mansoni. Protection of irradiated E. lindoense sporocysts by the same of different trematode species is presumed to be the result of an active process by which normal sporocysts interfere with capsule formation and protect themselves and other trematode larvae from encapsulation. Homologous protection was stronger than heterologous.", "PMID": 957039}
{"id": "pubmed23n0032_2534", "title": "New Oncomelania hupensis foci on Taiwan.", "content": "Six new areas for Oncomelania hupensis were found on Taiwan: three in Nan-tou, and 1 each in Chia-i, Hua-lien and Tai-tung counties. Most of the new habitats, unlike previously known areas, are not in alluvial plains, but are at higher elevations. The Nan-shan-chi colony in Nan-tou county is located at the altitude of 740 m, which is the highest of all known Oncomelania localities on the island. Oncomelania has now been found in 9 out of 15 counties on Taiwan. Shell features of the newly-discovered snails resemble those of Oncomelania hupensis formosana but further study will be required for final subspecies designation. Nearly 60,000 oncomelanids from both old and new localities were examined for schistosome infection with negative results.", "contents": "New Oncomelania hupensis foci on Taiwan. Six new areas for Oncomelania hupensis were found on Taiwan: three in Nan-tou, and 1 each in Chia-i, Hua-lien and Tai-tung counties. Most of the new habitats, unlike previously known areas, are not in alluvial plains, but are at higher elevations. The Nan-shan-chi colony in Nan-tou county is located at the altitude of 740 m, which is the highest of all known Oncomelania localities on the island. Oncomelania has now been found in 9 out of 15 counties on Taiwan. Shell features of the newly-discovered snails resemble those of Oncomelania hupensis formosana but further study will be required for final subspecies designation. Nearly 60,000 oncomelanids from both old and new localities were examined for schistosome infection with negative results.", "PMID": 957040}
{"id": "pubmed23n0032_2535", "title": "Cystobranchus meyeri sp. n. (Hirudinea: Piscicolidae) from Catostomus commersoni Lac\u00e9p\u00e8de in North America.", "content": "Cystobranchus meyeri sp. n. (Hirudinea: Piscicolidae) is described from the white sucker, Catostomus commersoni Lac\u00e9p\u00e8de (Osteichthyes: Catostomidae) from the Mohawk River drainage of eastern New York. Size, details of internal anatomy, and the presence of 8 ocelli on the posterior sucker distinguish it from the 3 other species in North America. A table compares the members of this genus and a key to the species is included. Problems of classification, host records, and host specificity are discussed.", "contents": "Cystobranchus meyeri sp. n. (Hirudinea: Piscicolidae) from Catostomus commersoni Lac\u00e9p\u00e8de in North America. Cystobranchus meyeri sp. n. (Hirudinea: Piscicolidae) is described from the white sucker, Catostomus commersoni Lac\u00e9p\u00e8de (Osteichthyes: Catostomidae) from the Mohawk River drainage of eastern New York. Size, details of internal anatomy, and the presence of 8 ocelli on the posterior sucker distinguish it from the 3 other species in North America. A table compares the members of this genus and a key to the species is included. Problems of classification, host records, and host specificity are discussed.", "PMID": 957041}
{"id": "pubmed23n0032_2536", "title": "Winter ecology of ectoparasites collected from hibernating Myotis velifer (Allen) in southwestern Oklahoma (Chiroptera: Vespertilionidae.", "content": "During the winter of 1971-72, Trichobius major, T, corynorhini, Mydopsylla collinsi, Macronyssus crosbyi, M. unidens, Paraspinturnix globosus, Spinturnix carloshoffmani, Ornithodoros sp., Albeckia senase, Nycteriglyphus sp. A, and Olabidocarpus sp. were quantitatively collected from hibernating Myotis velifer, Plecotus townsendii, and Pipistrellus subflavus. Significantly greater numbers of S.  carloshoffmani and M. crosbyi were found on female M. velifer, while greater numbers of P. globosus were found on males. With the exception of T. major and P. globosus which were radomly distributed, all ectoparasites exhibited a contagious distribution on M. velifer. Only T. major and S. carloshoffmani had a 1:1 sex ratio, the remaining species had significantly fewer males. M. unidens, M. crosbyi, and N. species A were positively associated, while M. collinsi was negatively associated with both Macronyssus; the remaining species were not signficantly associated. With the exception of T. major and P. globosus, ectoparasite densities decreased during the winter.", "contents": "Winter ecology of ectoparasites collected from hibernating Myotis velifer (Allen) in southwestern Oklahoma (Chiroptera: Vespertilionidae. During the winter of 1971-72, Trichobius major, T, corynorhini, Mydopsylla collinsi, Macronyssus crosbyi, M. unidens, Paraspinturnix globosus, Spinturnix carloshoffmani, Ornithodoros sp., Albeckia senase, Nycteriglyphus sp. A, and Olabidocarpus sp. were quantitatively collected from hibernating Myotis velifer, Plecotus townsendii, and Pipistrellus subflavus. Significantly greater numbers of S.  carloshoffmani and M. crosbyi were found on female M. velifer, while greater numbers of P. globosus were found on males. With the exception of T. major and P. globosus which were radomly distributed, all ectoparasites exhibited a contagious distribution on M. velifer. Only T. major and S. carloshoffmani had a 1:1 sex ratio, the remaining species had significantly fewer males. M. unidens, M. crosbyi, and N. species A were positively associated, while M. collinsi was negatively associated with both Macronyssus; the remaining species were not signficantly associated. With the exception of T. major and P. globosus, ectoparasite densities decreased during the winter.", "PMID": 957042}
{"id": "pubmed23n0032_2537", "title": "Prognostic factors in neuroblastic tumors.", "content": "Data of 101 patients with neuroblastic tumors from 1950 to1974 at The Johns Hopkins Hospital have been abstracted onto specially designed summary sheets, and subjected to multifactorial analysis. One-third of the 21 benign ganglioneuromas showed evidence for spontaneous maturation. The overall cure rate among the malignant tumors was 39%. Cures were higher with an extraabdominal primary, with more  mature histologic differentiation, and if excision, even partial, was carried out. Neither radiotherapy nor evolving modalities of chemotherapy appear to have had benefit over this 25-yr period. An age at diagnosis less than 2 yr, and a less extensive clinical stage both were independent predictors of survival. The apparent effects of a favorable site of tumor origin, or more mature histologic differentiation were not of major impact in and of themselves, but correlated better with clinical stage. Two prognostic schemata, based on stage plus age, and stage plus histologic differentiation plus age respectively, proved to be 95% accurate in  our experience. With more extensive evaluation and experience, either or both may be helpful in the management of malignant neuroblastic tumors, and assessment of therapeutic regimens.", "contents": "Prognostic factors in neuroblastic tumors. Data of 101 patients with neuroblastic tumors from 1950 to1974 at The Johns Hopkins Hospital have been abstracted onto specially designed summary sheets, and subjected to multifactorial analysis. One-third of the 21 benign ganglioneuromas showed evidence for spontaneous maturation. The overall cure rate among the malignant tumors was 39%. Cures were higher with an extraabdominal primary, with more  mature histologic differentiation, and if excision, even partial, was carried out. Neither radiotherapy nor evolving modalities of chemotherapy appear to have had benefit over this 25-yr period. An age at diagnosis less than 2 yr, and a less extensive clinical stage both were independent predictors of survival. The apparent effects of a favorable site of tumor origin, or more mature histologic differentiation were not of major impact in and of themselves, but correlated better with clinical stage. Two prognostic schemata, based on stage plus age, and stage plus histologic differentiation plus age respectively, proved to be 95% accurate in  our experience. With more extensive evaluation and experience, either or both may be helpful in the management of malignant neuroblastic tumors, and assessment of therapeutic regimens.", "PMID": 957051}
{"id": "pubmed23n0032_2538", "title": "Esophageal atresia and associated anomalies.", "content": "One hundred and two patients with esophageal  atresia possessed 237 additional anomalies. Risk grouping of these patients was useful and showed a direct relationship between the increased incidence of anomalies, low birth weight, and nonsurvival. Fifty-seven per cent of the patients with additional anomalies possessed multiple anomalies, most frequently occurring in the gastrointestinal, cardiovascular, and musculosketetal organ systems. Fifty-five per cent of the deaths appeared to result from the coexistent severe anomaly, while the remaining 45% of the deaths appeared to occur as a result of the presence of esophageal atresia. It is our suspicion that the insult producing esophageal atresia with a high incidence of associated anomalies occurs at a more critical time in organogenesis than that event that produces the esophageal atresis alone or with one moderate anomaly.", "contents": "Esophageal atresia and associated anomalies. One hundred and two patients with esophageal  atresia possessed 237 additional anomalies. Risk grouping of these patients was useful and showed a direct relationship between the increased incidence of anomalies, low birth weight, and nonsurvival. Fifty-seven per cent of the patients with additional anomalies possessed multiple anomalies, most frequently occurring in the gastrointestinal, cardiovascular, and musculosketetal organ systems. Fifty-five per cent of the deaths appeared to result from the coexistent severe anomaly, while the remaining 45% of the deaths appeared to occur as a result of the presence of esophageal atresia. It is our suspicion that the insult producing esophageal atresia with a high incidence of associated anomalies occurs at a more critical time in organogenesis than that event that produces the esophageal atresis alone or with one moderate anomaly.", "PMID": 957052}
{"id": "pubmed23n0032_2539", "title": "Continuous dynamic monitoring of pressure and flow patterns during assisted ventilation.", "content": "A system for continuous, dynamic monitoring of children requiring ventilatory assistance  has been developed and used in a critical care setting. Breath by breath airway pressure changes are measured on a specially adapted meter with high-and low-pressure limit alarms, and are displayed graphically on a bedside oscilloscope. This system has proved to be beneficial in detecting ventilator system malfunction; in adjusting ventilator settings, such as inspiratory/expiratory ratios, end-expiratory pressure, and phase duration and flow  ratios; in assessing compliance of a child requiring ventilatory assistance and his capability for spontaneous breathing; and in the early recognition of potentially harmful problems attendant upon assisted ventilation. The system provides additional educational benefit for the I.C.U. personnel, who become more knowledgeable regarding ventilatory problems, and more apt to discover them promptly. The simplicity of the system, compatibility with other I.C.U. monitoring equipment, capability for continuous, dynamic monitoring, and broad scope recommended it for infants and children requiring assisted ventilation.", "contents": "Continuous dynamic monitoring of pressure and flow patterns during assisted ventilation. A system for continuous, dynamic monitoring of children requiring ventilatory assistance  has been developed and used in a critical care setting. Breath by breath airway pressure changes are measured on a specially adapted meter with high-and low-pressure limit alarms, and are displayed graphically on a bedside oscilloscope. This system has proved to be beneficial in detecting ventilator system malfunction; in adjusting ventilator settings, such as inspiratory/expiratory ratios, end-expiratory pressure, and phase duration and flow  ratios; in assessing compliance of a child requiring ventilatory assistance and his capability for spontaneous breathing; and in the early recognition of potentially harmful problems attendant upon assisted ventilation. The system provides additional educational benefit for the I.C.U. personnel, who become more knowledgeable regarding ventilatory problems, and more apt to discover them promptly. The simplicity of the system, compatibility with other I.C.U. monitoring equipment, capability for continuous, dynamic monitoring, and broad scope recommended it for infants and children requiring assisted ventilation.", "PMID": 957053}
{"id": "pubmed23n0032_2540", "title": "Serum gastrin concentrations in infants with short gut syndrome.", "content": "Seven babies with 100 cm or less remaining small bowel have been evaluated for evidence of gastric hyperacidity and/or hypergastrinemia. Two babies were also studied after feeding. No patient demonstrated hyperacidity or hypergastrinemia. This infant data is discussed in regards to reported contradictory data in adult studies.", "contents": "Serum gastrin concentrations in infants with short gut syndrome. Seven babies with 100 cm or less remaining small bowel have been evaluated for evidence of gastric hyperacidity and/or hypergastrinemia. Two babies were also studied after feeding. No patient demonstrated hyperacidity or hypergastrinemia. This infant data is discussed in regards to reported contradictory data in adult studies.", "PMID": 957054}
{"id": "pubmed23n0032_2541", "title": "Intestinal stricture in necrotizing enterocolitis.", "content": "In a 3-yr period, eight infants among 43 survivors of acute NEC developed intestinal stricture. Four infants developed multiple stricture after proximal diversion procedures, and four had single strictures after medical therapy. Nineteen cases of intestinal stricture after NEC were collected from the literature. Radiographic examinations at the time of the acute disease were not predictive of the risk of subsequent stricture. Histologic examination showed various stages of wound healing, most prominently in the submucosa. Stricture should be considered as the cause of intestinal malfunction in any child who survives acute NEC.", "contents": "Intestinal stricture in necrotizing enterocolitis. In a 3-yr period, eight infants among 43 survivors of acute NEC developed intestinal stricture. Four infants developed multiple stricture after proximal diversion procedures, and four had single strictures after medical therapy. Nineteen cases of intestinal stricture after NEC were collected from the literature. Radiographic examinations at the time of the acute disease were not predictive of the risk of subsequent stricture. Histologic examination showed various stages of wound healing, most prominently in the submucosa. Stricture should be considered as the cause of intestinal malfunction in any child who survives acute NEC.", "PMID": 957055}
{"id": "pubmed23n0032_2542", "title": "Primary subacute pyogenic osteomyelitis in long bones of children.", "content": "Ten cases of primary subacute osteomyelitis illustrate its clinical resemblance to various bone neoplasms. Diagnosis was established by open biopsy. Treatment with curettage, cast immobilization, and antibiotics, when indicated, resulted in radiographic healing and resolution of symptoms.", "contents": "Primary subacute pyogenic osteomyelitis in long bones of children. Ten cases of primary subacute osteomyelitis illustrate its clinical resemblance to various bone neoplasms. Diagnosis was established by open biopsy. Treatment with curettage, cast immobilization, and antibiotics, when indicated, resulted in radiographic healing and resolution of symptoms.", "PMID": 957057}
{"id": "pubmed23n0032_2543", "title": "Primary surgical management of the deeply burned hand in children.", "content": "Primary burn excision with immediate wound closure by skin grafting was used as the initial definitive treatment for deep burns of the hand in 30 consecutive  children. The results are compared with an additional group of 30 children whose burns were treated with AgNO3 alone. There is a significant difference in all categories examined between the excised and the nonexcised group. Hand deformities, secondary reconstructive procedures, and morbidity  were significantly reduced in the excised group. Excision provided improved functional and cosmetic results.", "contents": "Primary surgical management of the deeply burned hand in children. Primary burn excision with immediate wound closure by skin grafting was used as the initial definitive treatment for deep burns of the hand in 30 consecutive  children. The results are compared with an additional group of 30 children whose burns were treated with AgNO3 alone. There is a significant difference in all categories examined between the excised and the nonexcised group. Hand deformities, secondary reconstructive procedures, and morbidity  were significantly reduced in the excised group. Excision provided improved functional and cosmetic results.", "PMID": 957058}
{"id": "pubmed23n0032_2544", "title": "Wringer arm injuries.", "content": "During a 20-yr period, 483 children 16 yr of age or under were hospitalized for management of a wringer arm injury. Although the majority were indigent, black males, such trauma was frequently sustained by all classes and races as well as by both sexes. Principles of treatment consisted of cleansing the abrasion wound, application of a pressure dressing from axilla to finger tips, elevation, and close observation. With the exception of a destroyed brachial vein in one patien, significant vascular injuries  did not occur despite a \"medical fixation\" on evidences of impaired circulation. Likewise, no fractures were noted even though all patients had x-rays of the full extremity. However, there were six metacarpophalangeal dislocations, five instances of neuropathy, and one traumatic amputation. Major skin sloughs occurred in 18 children, yet only 11 of them required a skin graft.", "contents": "Wringer arm injuries. During a 20-yr period, 483 children 16 yr of age or under were hospitalized for management of a wringer arm injury. Although the majority were indigent, black males, such trauma was frequently sustained by all classes and races as well as by both sexes. Principles of treatment consisted of cleansing the abrasion wound, application of a pressure dressing from axilla to finger tips, elevation, and close observation. With the exception of a destroyed brachial vein in one patien, significant vascular injuries  did not occur despite a \"medical fixation\" on evidences of impaired circulation. Likewise, no fractures were noted even though all patients had x-rays of the full extremity. However, there were six metacarpophalangeal dislocations, five instances of neuropathy, and one traumatic amputation. Major skin sloughs occurred in 18 children, yet only 11 of them required a skin graft.", "PMID": 957061}
{"id": "pubmed23n0032_2545", "title": "Modified Duhamel procedure for treatment of total aganglionic colon in childhood.", "content": "Based on this series of six patients with aganglionosis  of the entire colon we conclude: (1) Radiographic findings of a shortened colon of normal caliber or the presence of \"jejunalization\" of the colon suggest total colonic aganglionosis in patients with a suggestive history. (2) All infants with persistent obstipation, distention, and poor weight gain should have a punch biopsy of the rectum even if the barium enema is normal. (3) The Martin modification of Duhamel's operation gives functional results comparable to those achievable in children with short-segment Hirschsprung's disease. (4) The use of a stapling device to divide the septum between aganglionic colon and pulled-through  ileum is less satisfactory than using crushing clamps.", "contents": "Modified Duhamel procedure for treatment of total aganglionic colon in childhood. Based on this series of six patients with aganglionosis  of the entire colon we conclude: (1) Radiographic findings of a shortened colon of normal caliber or the presence of \"jejunalization\" of the colon suggest total colonic aganglionosis in patients with a suggestive history. (2) All infants with persistent obstipation, distention, and poor weight gain should have a punch biopsy of the rectum even if the barium enema is normal. (3) The Martin modification of Duhamel's operation gives functional results comparable to those achievable in children with short-segment Hirschsprung's disease. (4) The use of a stapling device to divide the septum between aganglionic colon and pulled-through  ileum is less satisfactory than using crushing clamps.", "PMID": 957063}
{"id": "pubmed23n0032_2546", "title": "Jejunoileal atresia: a proposed classification.", "content": "An experience with 59 infants with jejunal and ileal atresia is presented along with a proposed classification based on a combination of morphology and clinical characteristics. It would appear that the characteristics of each type are sufficiently distinct as to render this worthwhile classification.", "contents": "Jejunoileal atresia: a proposed classification. An experience with 59 infants with jejunal and ileal atresia is presented along with a proposed classification based on a combination of morphology and clinical characteristics. It would appear that the characteristics of each type are sufficiently distinct as to render this worthwhile classification.", "PMID": 957064}
{"id": "pubmed23n0032_2547", "title": "Ruptured appendicitis in children: continuing controversy over antibiotic combinations.", "content": "A prospective study has been made of 36 children with ruptured appendicitis. Alternate patients were managed with a standard protocol of therapy differing only in the antibiotics used. One group received a combination of penicillin/streptomycin/sulfadiazine while the other children were treated with cephalothin (Keflin) and cephalexin (Keflex). No major infections complications occurred in the P/S/S study group but there were four in the C/C patients. No serious adverse reactions due to the antibiotics occurred in either group.", "contents": "Ruptured appendicitis in children: continuing controversy over antibiotic combinations. A prospective study has been made of 36 children with ruptured appendicitis. Alternate patients were managed with a standard protocol of therapy differing only in the antibiotics used. One group received a combination of penicillin/streptomycin/sulfadiazine while the other children were treated with cephalothin (Keflin) and cephalexin (Keflex). No major infections complications occurred in the P/S/S study group but there were four in the C/C patients. No serious adverse reactions due to the antibiotics occurred in either group.", "PMID": 957065}
{"id": "pubmed23n0032_2548", "title": "Are hemisplenectomy and/or primary splenic repair feasible?", "content": "Following splenectomy, infant rats are at risk when challenged with pneumococcus. Mortality was 85% in splenectomized rats and 6.6% in controls. Rats subjected to a standardized splenic trauma had a 16% mortality when left untreated and a zero mortality with operative therapy by splenectomy, hemisplenectomy, or primary splenic repair. Hemisplenectomy and primary splenic repair are indeed feasible in rats with resultant splenic tissue showing a normal histologic appearance. Hemisplenectomized infant rats, when subjected to pneumococcal challenge, had a 15% mortality compared to 85% for splenectomized infant rats. This suggests that the repaired spleen retains its very important immunologic abiltiy to resist infection. Despite differences in species and organ sizes and shapes, these data suggest that partial splenectomy and primary splenic repair may be attractive alternatives to splenectomy in instances of trauma and  inadvertant operative injury.", "contents": "Are hemisplenectomy and/or primary splenic repair feasible? Following splenectomy, infant rats are at risk when challenged with pneumococcus. Mortality was 85% in splenectomized rats and 6.6% in controls. Rats subjected to a standardized splenic trauma had a 16% mortality when left untreated and a zero mortality with operative therapy by splenectomy, hemisplenectomy, or primary splenic repair. Hemisplenectomy and primary splenic repair are indeed feasible in rats with resultant splenic tissue showing a normal histologic appearance. Hemisplenectomized infant rats, when subjected to pneumococcal challenge, had a 15% mortality compared to 85% for splenectomized infant rats. This suggests that the repaired spleen retains its very important immunologic abiltiy to resist infection. Despite differences in species and organ sizes and shapes, these data suggest that partial splenectomy and primary splenic repair may be attractive alternatives to splenectomy in instances of trauma and  inadvertant operative injury.", "PMID": 957066}
{"id": "pubmed23n0032_2549", "title": "Repair of laryngotracheoesophageal cleft using interposition of a strap muscle.", "content": "Two  children with laryngotracheoesophageal clefts were operated with success. In one case the cleft resulted from trauma. The second case was of  congenital origin and had been operated twice previously. Interposition of the sternohyoid muscle was an important adjuct contributing to success in repair.", "contents": "Repair of laryngotracheoesophageal cleft using interposition of a strap muscle. Two  children with laryngotracheoesophageal clefts were operated with success. In one case the cleft resulted from trauma. The second case was of  congenital origin and had been operated twice previously. Interposition of the sternohyoid muscle was an important adjuct contributing to success in repair.", "PMID": 957067}
{"id": "pubmed23n0032_2550", "title": "Endoscopic resection of obstructing airway lesions in children.", "content": "In the past 8 yrs, 14 children were treated with obstructing lesions of the larynx, some of then acquired from prolonged intubation. Endoscopic resection of the obstructions proved feasible in selected cases. The use of a wire stylette of fine needle within a small ureteral catheter sheath provides a  readily available and accurate means for dealing with some  of these lesions.", "contents": "Endoscopic resection of obstructing airway lesions in children. In the past 8 yrs, 14 children were treated with obstructing lesions of the larynx, some of then acquired from prolonged intubation. Endoscopic resection of the obstructions proved feasible in selected cases. The use of a wire stylette of fine needle within a small ureteral catheter sheath provides a  readily available and accurate means for dealing with some  of these lesions.", "PMID": 957068}
{"id": "pubmed23n0032_2551", "title": "Clinical technique and success of the esophageal stent to prevent corrosive strictures.", "content": "The Reyes technique for the management of severe, acute  corrosive injuries of the esophagus has been modified and successfully applied to two children and one adult whose cases are reported. None developed stricture and two were uncomplicated. Esophagomegaly, motility dysfunction, upper gastrointestinal bleeding, and pneumonitis complicated the hospitalization of one child whose subsequent course has been asymptomatic. Until more experience can be recorded, widespread use is not yet recommended.", "contents": "Clinical technique and success of the esophageal stent to prevent corrosive strictures. The Reyes technique for the management of severe, acute  corrosive injuries of the esophagus has been modified and successfully applied to two children and one adult whose cases are reported. None developed stricture and two were uncomplicated. Esophagomegaly, motility dysfunction, upper gastrointestinal bleeding, and pneumonitis complicated the hospitalization of one child whose subsequent course has been asymptomatic. Until more experience can be recorded, widespread use is not yet recommended.", "PMID": 957069}
{"id": "pubmed23n0032_2552", "title": "Changes in appearance of amniotic fluid during pregnancy - the macroscore.", "content": "During the first two trimesters of pregnancy the amniotic fluid is clear and yellow; during the third trimester the amniotic fluid becomes colourless; then, approximately from the 33rd-34rd week on, cloudiness and flocculation occur, at first very slowly, after the 36th-37th week steadily faster (Tab. I). At term, the amniotic fluid is moderately cloudy and contains a moderate number of flakes of vernix. The appearance of the amniotic fluid depending on the degree of cloudiness and on the number of flakes, has been expressed by means of a score system, the socalled macroscore (Tab. II). Relationships were observed: a) between the disappearance of the yellow colour (bilirubin) and the initial occurrence of cloudiness and flocculation; b) between the duration of pregnancy and the macroscore; from the 32nd-36th week of pregnancy the mean macroscore increases until the second half of the 40th week; then in the 41 st week there is a drop in the mean macroscore, after which a new increase occurs (Fig. 1,2 and 3). c) between the total gestation period at birth and the progression of the macroscore (Fig. 5); when birth takes place earlier (later), the macroscore will increase earlier (later). d) between the total duration of gestation at birth and the macroscore at the end of pregnancy; with an earlier (later) birth, the macroscore is lower (higher) (Fig. 5 and 6). With the macroscore it is possible to determine the duration of pregnancy (b) and the time before birth even more accurately (c). The fairly large standard deviation of the macroscore per pregnancy week (Fig. 1) also in case of a given duration of gestation at birth (Fig. 7) points to a fairly large interindividual variation in the appearance of the amniotic fluid at a certain duration of pregnancy. The macroscore is determined by elements originating from the fetal skin; the cloudiness and flocculation are caused by release of vernix and the flaking off of cells from the stratum corneum. Hence the macroscore reflects changes in the function of the fetal skin and is an indicator of the functional maturation of the fetal skin. The considerable variation of the macroscore at a given duration of pregnancy indicates a great variation of fetal maturation. The fetus that is maturing faster, will be delivered earlier; the fetus that  is maturing slower, later (c). This points to a correlation between the degree of fetal maturation and the start of labour. The higher macroscore during the last days before birth in pregnancies of longer duration (d) (Fig. 5 and 6) may be explained by a less sensitive uterus, requiring a greater maturity of the fetus for delivery to start. The drop of the mean macroscore in the 41 st week of pregnancy is due to a sudden increase of lower scores in this week (Fig. 4). A lower score at a given stage of pregnancy means a later birth (Tab. VI and VII). Thus in the 41 st week of pregnancy a considerable group of pregnant women appears, that has a total duration of gestation that is, on the average, two weeks longer than normally...", "contents": "Changes in appearance of amniotic fluid during pregnancy - the macroscore. During the first two trimesters of pregnancy the amniotic fluid is clear and yellow; during the third trimester the amniotic fluid becomes colourless; then, approximately from the 33rd-34rd week on, cloudiness and flocculation occur, at first very slowly, after the 36th-37th week steadily faster (Tab. I). At term, the amniotic fluid is moderately cloudy and contains a moderate number of flakes of vernix. The appearance of the amniotic fluid depending on the degree of cloudiness and on the number of flakes, has been expressed by means of a score system, the socalled macroscore (Tab. II). Relationships were observed: a) between the disappearance of the yellow colour (bilirubin) and the initial occurrence of cloudiness and flocculation; b) between the duration of pregnancy and the macroscore; from the 32nd-36th week of pregnancy the mean macroscore increases until the second half of the 40th week; then in the 41 st week there is a drop in the mean macroscore, after which a new increase occurs (Fig. 1,2 and 3). c) between the total gestation period at birth and the progression of the macroscore (Fig. 5); when birth takes place earlier (later), the macroscore will increase earlier (later). d) between the total duration of gestation at birth and the macroscore at the end of pregnancy; with an earlier (later) birth, the macroscore is lower (higher) (Fig. 5 and 6). With the macroscore it is possible to determine the duration of pregnancy (b) and the time before birth even more accurately (c). The fairly large standard deviation of the macroscore per pregnancy week (Fig. 1) also in case of a given duration of gestation at birth (Fig. 7) points to a fairly large interindividual variation in the appearance of the amniotic fluid at a certain duration of pregnancy. The macroscore is determined by elements originating from the fetal skin; the cloudiness and flocculation are caused by release of vernix and the flaking off of cells from the stratum corneum. Hence the macroscore reflects changes in the function of the fetal skin and is an indicator of the functional maturation of the fetal skin. The considerable variation of the macroscore at a given duration of pregnancy indicates a great variation of fetal maturation. The fetus that is maturing faster, will be delivered earlier; the fetus that  is maturing slower, later (c). This points to a correlation between the degree of fetal maturation and the start of labour. The higher macroscore during the last days before birth in pregnancies of longer duration (d) (Fig. 5 and 6) may be explained by a less sensitive uterus, requiring a greater maturity of the fetus for delivery to start. The drop of the mean macroscore in the 41 st week of pregnancy is due to a sudden increase of lower scores in this week (Fig. 4). A lower score at a given stage of pregnancy means a later birth (Tab. VI and VII). Thus in the 41 st week of pregnancy a considerable group of pregnant women appears, that has a total duration of gestation that is, on the average, two weeks longer than normally...", "PMID": 957078}
{"id": "pubmed23n0032_2553", "title": "Problems in using linear ultrasound parameters for the determination of fetal weight.", "content": "We have developed three formulas for the calculation of fetal weight by means of ultrasound which have proved clinically useful   (Formula I for normal size fetuses, Formula II for very small fetuses, and Formula III as a control). From 621 controlled calculations of the fetal weight and the general experience from our ultrasound clinic (over 20,000 examinations) we discuss the inherent problems. We used the Vidoson apparatus manufactured by Siemens (West Germany) which is a rapid imaging B-scan. We have learned from many years of studying means of calculating fetal weight that a regression equation from linear fetal measurements can be used: namely the biparietelllll skull diameter, the sagittal and the transverse thoracic diameter and the length of the trunk (Tab. I)...", "contents": "Problems in using linear ultrasound parameters for the determination of fetal weight. We have developed three formulas for the calculation of fetal weight by means of ultrasound which have proved clinically useful   (Formula I for normal size fetuses, Formula II for very small fetuses, and Formula III as a control). From 621 controlled calculations of the fetal weight and the general experience from our ultrasound clinic (over 20,000 examinations) we discuss the inherent problems. We used the Vidoson apparatus manufactured by Siemens (West Germany) which is a rapid imaging B-scan. We have learned from many years of studying means of calculating fetal weight that a regression equation from linear fetal measurements can be used: namely the biparietelllll skull diameter, the sagittal and the transverse thoracic diameter and the length of the trunk (Tab. I)...", "PMID": 957079}
{"id": "pubmed23n0032_2554", "title": "Prospective and retrospective examination of an easily applicable score to predict the probability of premature birth defined by weight.", "content": "An easily applicable score to predict the risk of prematurity (Tab. I) (defined by weight) is examined prospectively (scoring during 6 th month of pregnancy) in 431 and retrospectively (obtained after delivery) in 1183 pregnancies. In the prospective study (Tab. II) 71.4% of all pregnancies resulting in babies below 2501 g exceed the proposed 50 points risk probabilty limit whereas only 18.7% of pregnancies with babies of more than 3000 g do so. Excluding pregnancies with 20 or more risk points and excellent prenatal care (8 or more consultations) - which should change the outcome of risk-pregnancies - the percentages are 77.8% and 12.2% respectively (Tab. III). Pregnancies resulting in babies with birth weight of 2501 g -2750 g exceeded the limit in 38.9% and those with babies of 2751 g-3000 g in 20.7%. If 60 risk points are used as the limit the percentages for more than 3000 g until less than 2501 g would be 8.2%, 6.9%, 33.3% and 66.7%. In the resrospective study (Tab. V) 14.7% of all pregnancies with babies above 3000 g exceeded the 50 risk points limit compared with 57.2 of those with babies below 2501 g. Excluding pregnancies with 20 or more risk points and excellent prental care the percentages are 7.6 and 59.4 respectively. In the retrospective study the influence of the quality of prenatal care by the number of consultations (3-4; 5-7; 8 or more) is clearly demonstrable: Pregnancies with more than 50 risk points resulted in 80.7%, 57.1% and 19.8% depending on the quality of care in babies below 2501 g. Pregnancies with 31-50 risk points did so in 47.2%, 20.4% and 11.8%. In 334 women the score could be applied twice, in the 6th month and at delivery. Comparing both scores it was found that only 1.8% of these women exceeded the 50 risk points limit by events occurring after the 6th month scoring (Tab. IV). The score, simple enough to be applied by nurses and midwives, seems to be able to select 77.8% of pregnancies resulting in babies below 2501 g already during the 6th month of pregnancy, i.e. early enough for preventive measures to be taken that decrease the frequency of underweight births by three quarters.", "contents": "Prospective and retrospective examination of an easily applicable score to predict the probability of premature birth defined by weight. An easily applicable score to predict the risk of prematurity (Tab. I) (defined by weight) is examined prospectively (scoring during 6 th month of pregnancy) in 431 and retrospectively (obtained after delivery) in 1183 pregnancies. In the prospective study (Tab. II) 71.4% of all pregnancies resulting in babies below 2501 g exceed the proposed 50 points risk probabilty limit whereas only 18.7% of pregnancies with babies of more than 3000 g do so. Excluding pregnancies with 20 or more risk points and excellent prenatal care (8 or more consultations) - which should change the outcome of risk-pregnancies - the percentages are 77.8% and 12.2% respectively (Tab. III). Pregnancies resulting in babies with birth weight of 2501 g -2750 g exceeded the limit in 38.9% and those with babies of 2751 g-3000 g in 20.7%. If 60 risk points are used as the limit the percentages for more than 3000 g until less than 2501 g would be 8.2%, 6.9%, 33.3% and 66.7%. In the resrospective study (Tab. V) 14.7% of all pregnancies with babies above 3000 g exceeded the 50 risk points limit compared with 57.2 of those with babies below 2501 g. Excluding pregnancies with 20 or more risk points and excellent prental care the percentages are 7.6 and 59.4 respectively. In the retrospective study the influence of the quality of prenatal care by the number of consultations (3-4; 5-7; 8 or more) is clearly demonstrable: Pregnancies with more than 50 risk points resulted in 80.7%, 57.1% and 19.8% depending on the quality of care in babies below 2501 g. Pregnancies with 31-50 risk points did so in 47.2%, 20.4% and 11.8%. In 334 women the score could be applied twice, in the 6th month and at delivery. Comparing both scores it was found that only 1.8% of these women exceeded the 50 risk points limit by events occurring after the 6th month scoring (Tab. IV). The score, simple enough to be applied by nurses and midwives, seems to be able to select 77.8% of pregnancies resulting in babies below 2501 g already during the 6th month of pregnancy, i.e. early enough for preventive measures to be taken that decrease the frequency of underweight births by three quarters.", "PMID": 957080}
{"id": "pubmed23n0032_2555", "title": "Fetal paroxysaml supraventricular tachycardia recorded by intrauterine scalp electrocardiography.", "content": "Fetal and neonatal paroxysmal supraventricular tachycardia (PST) requires attention because it may be associated with cardiac pathology and neonatal demise. Continuous monitoring of fetal heart rate and the ability to record clearly interpretable electrocardiograms from fetal scalp electrodes makes discovery and diagnoses of these fetal arrhythmias relatively simple. This report describes the discovery of this arrhythmia during routine DOPPLER ultrasound fetal monitoring and its documentation as PST by fetal scalp electrocardiography. (Fig. 1) The fetal heart rate during PST was 300 BPM, higher than previously reported. In contrast to previous reports, it did not appear post-natally and no pathology was found. Although the significance of transient fetal PST is not known, prudence indicates close observation of the neonate. This case demonstrates the value of fetal monitoring for the discovery, diagnosis and documentation of fetal arrhythmias during labor.", "contents": "Fetal paroxysaml supraventricular tachycardia recorded by intrauterine scalp electrocardiography. Fetal and neonatal paroxysmal supraventricular tachycardia (PST) requires attention because it may be associated with cardiac pathology and neonatal demise. Continuous monitoring of fetal heart rate and the ability to record clearly interpretable electrocardiograms from fetal scalp electrodes makes discovery and diagnoses of these fetal arrhythmias relatively simple. This report describes the discovery of this arrhythmia during routine DOPPLER ultrasound fetal monitoring and its documentation as PST by fetal scalp electrocardiography. (Fig. 1) The fetal heart rate during PST was 300 BPM, higher than previously reported. In contrast to previous reports, it did not appear post-natally and no pathology was found. Although the significance of transient fetal PST is not known, prudence indicates close observation of the neonate. This case demonstrates the value of fetal monitoring for the discovery, diagnosis and documentation of fetal arrhythmias during labor.", "PMID": 957081}
{"id": "pubmed23n0032_2556", "title": "Perceptual development on the Rorschach.", "content": "The Rorschach was given to 60 school children in two designs: CA and MA orthogonal (9 and 12 years) and CA =MA (for 6, 9 and 12 years). Responses were scored for Form Accuracy, Complexity, Movement, and Friedman's Developmental Level (DL) Scoring System. There were significant MA effects independent of CA on Form Accuracy, Complexity and DL, and significant CA effects on Complexity and Movement. Form Accuracy was highly correlated with the DL score and correlated with MA at a slightly higher level than DL with MA. The Complexity score provided some additional information about development. Movement decreased with age but was higher in intelligent subjects. The results suggested that the DL system does assess MA independently of CA, but this is accounted for primarily by Form Accuracy.", "contents": "Perceptual development on the Rorschach. The Rorschach was given to 60 school children in two designs: CA and MA orthogonal (9 and 12 years) and CA =MA (for 6, 9 and 12 years). Responses were scored for Form Accuracy, Complexity, Movement, and Friedman's Developmental Level (DL) Scoring System. There were significant MA effects independent of CA on Form Accuracy, Complexity and DL, and significant CA effects on Complexity and Movement. Form Accuracy was highly correlated with the DL score and correlated with MA at a slightly higher level than DL with MA. The Complexity score provided some additional information about development. Movement decreased with age but was higher in intelligent subjects. The results suggested that the DL system does assess MA independently of CA, but this is accounted for primarily by Form Accuracy.", "PMID": 957082}
{"id": "pubmed23n0032_2557", "title": "The relationship between behavioral indices of aggression and hostile content on the TAT.", "content": "The role played by fantasy in mitigating the overt expression of aggression was investigated by examining the relationship between aggressive behavior and aggressive story content on the Thematic Apperception Test. A rating scale, checklist, and offense scale were used to measure the aggressive behavior of 76 juvenile offenders. While only one measure correlated significantly with hostile content on the TAT, all three correlations were negative, suggesting an inverse relationship between measure of aggression and aggressive content on the TAT.", "contents": "The relationship between behavioral indices of aggression and hostile content on the TAT. The role played by fantasy in mitigating the overt expression of aggression was investigated by examining the relationship between aggressive behavior and aggressive story content on the Thematic Apperception Test. A rating scale, checklist, and offense scale were used to measure the aggressive behavior of 76 juvenile offenders. While only one measure correlated significantly with hostile content on the TAT, all three correlations were negative, suggesting an inverse relationship between measure of aggression and aggressive content on the TAT.", "PMID": 957083}
{"id": "pubmed23n0032_2558", "title": "Interrelationships of psychopathology and adience-abience on the HABGT.", "content": "Using two distinctively different populations, 40 hospitalized schizophrenics and 100 outpatient therapy cases, the interrelationships of two measures based on the HABGT, one of psychopathology and the other of perceptual adience-abience, were investigated. The relationships of each of these measures with sex, age, and educational level were examined. Both measures significantly differentiate the psychotic from the non-psychotic population. Although there is a statistically significant correlation between the two measures in each population, it is shown that the commonality between them varies from a considerable degree of commonality in the schizophrenic group to only a limited degree in the outpatient group. Both measures are uncorrelated with sex, age, and level of educational attainment. The study offers additional evidence of the values of these two measures.", "contents": "Interrelationships of psychopathology and adience-abience on the HABGT. Using two distinctively different populations, 40 hospitalized schizophrenics and 100 outpatient therapy cases, the interrelationships of two measures based on the HABGT, one of psychopathology and the other of perceptual adience-abience, were investigated. The relationships of each of these measures with sex, age, and educational level were examined. Both measures significantly differentiate the psychotic from the non-psychotic population. Although there is a statistically significant correlation between the two measures in each population, it is shown that the commonality between them varies from a considerable degree of commonality in the schizophrenic group to only a limited degree in the outpatient group. Both measures are uncorrelated with sex, age, and level of educational attainment. The study offers additional evidence of the values of these two measures.", "PMID": 957084}
{"id": "pubmed23n0032_2559", "title": "Anxiety and defense against anxiety as reflected in percept-genetic formations.", "content": "Two percept-genetic methods, the Meta-Contrast Technique (MCT) and the Serial Afterimage Test (AI), were used to study adaptive aspects of perception in a group of 37 subjects suffering from manifest anxiety. The perceptual processes laid bare by these methods reflected not only anxiety but also defensive formations. MCT and AI correlated with each other in these respects and, when combined, correlated with rated symptoms. In accordance with the theory behind percept-genetic methods, anxiety signs appearing late in the test serials were more likely to correspond to manifest symptoms than signs which appeared early. The AI test scores seemed to reflect a difference between anxiety experienced as an inherent quality of life and anxiety as a foreign force struggling for dominance.", "contents": "Anxiety and defense against anxiety as reflected in percept-genetic formations. Two percept-genetic methods, the Meta-Contrast Technique (MCT) and the Serial Afterimage Test (AI), were used to study adaptive aspects of perception in a group of 37 subjects suffering from manifest anxiety. The perceptual processes laid bare by these methods reflected not only anxiety but also defensive formations. MCT and AI correlated with each other in these respects and, when combined, correlated with rated symptoms. In accordance with the theory behind percept-genetic methods, anxiety signs appearing late in the test serials were more likely to correspond to manifest symptoms than signs which appeared early. The AI test scores seemed to reflect a difference between anxiety experienced as an inherent quality of life and anxiety as a foreign force struggling for dominance.", "PMID": 957085}
{"id": "pubmed23n0032_2560", "title": "Associative elaboration and integration scales for evaluating TAT protocols.", "content": "An Associative Elaboration Scale and an Integration Scale were constructed for evaluating Thematic Apperception Test (TAT) protocols. Interrater reliabilities and the correlation between the two scales were determined for a selection of TAT cards. The Integration Scale was dropped from further analyses, because its interrater reliabilities were low and it correlated highly with the Associative Elaboration Scale. The relation between the Associative Elaboration Scale and age was then investigated for randomly selected patients aged 7, 9, 11, 13, and 15. There tended to be a rapid increase in Associative Elaboration scores up to age 11 and then little or no increase in scores. There was no significant relation between Associative Elaboration and IQ.", "contents": "Associative elaboration and integration scales for evaluating TAT protocols. An Associative Elaboration Scale and an Integration Scale were constructed for evaluating Thematic Apperception Test (TAT) protocols. Interrater reliabilities and the correlation between the two scales were determined for a selection of TAT cards. The Integration Scale was dropped from further analyses, because its interrater reliabilities were low and it correlated highly with the Associative Elaboration Scale. The relation between the Associative Elaboration Scale and age was then investigated for randomly selected patients aged 7, 9, 11, 13, and 15. There tended to be a rapid increase in Associative Elaboration scores up to age 11 and then little or no increase in scores. There was no significant relation between Associative Elaboration and IQ.", "PMID": 957086}
{"id": "pubmed23n0032_2561", "title": "Decisiveness and psychological adjustment.", "content": "Decisiveness, defined as the ability of the individual to engage in the decision-making process, was examined in relation to a number of parameters of psychological adjustment. Six decisiveness instruments, a series of adjustment measures, and peer rating assessments were obtained for 228 volunteer subjects. The pattern of relationships between the decisiveness measures suggested that decisiveness is not a monolithic trait, but rather is multidimensional. The relationship between the decisiveness and adjustment, with the impressionistic, global measures of decisiveness showing a stronger relationship to adjustment than the behavioral, objective ones. However, contamination of the data due to methods factors was observed, and sex was seen to be a factor in the interaction between decisiveness and adjustment. Methodological improvements and suggestions for further research are offered.", "contents": "Decisiveness and psychological adjustment. Decisiveness, defined as the ability of the individual to engage in the decision-making process, was examined in relation to a number of parameters of psychological adjustment. Six decisiveness instruments, a series of adjustment measures, and peer rating assessments were obtained for 228 volunteer subjects. The pattern of relationships between the decisiveness measures suggested that decisiveness is not a monolithic trait, but rather is multidimensional. The relationship between the decisiveness and adjustment, with the impressionistic, global measures of decisiveness showing a stronger relationship to adjustment than the behavioral, objective ones. However, contamination of the data due to methods factors was observed, and sex was seen to be a factor in the interaction between decisiveness and adjustment. Methodological improvements and suggestions for further research are offered.", "PMID": 957087}
{"id": "pubmed23n0032_2562", "title": "Correlates of risky decision-making.", "content": "The purpose of this investigation was to develop a personality pattern which could be used for both the explication and prediction of risky behavior in a variety of decision-making situations. Two months after the completion of a battery of psychological examinations, 240 randomly selected subjects responded to three problems adapted from the Kogan and Wallach Choice Dilemma Problems. Analyses of the data (correlational, factor analytic, and stepwise multiple regression) revealed a stable personality index representative of individuals exhibiting riskiness in decision-making. Variables contributing to this pattern characterized a dynamic task oriented individual. The results of this study should prove useful to future decision-making research by providing a framework for prediction.", "contents": "Correlates of risky decision-making. The purpose of this investigation was to develop a personality pattern which could be used for both the explication and prediction of risky behavior in a variety of decision-making situations. Two months after the completion of a battery of psychological examinations, 240 randomly selected subjects responded to three problems adapted from the Kogan and Wallach Choice Dilemma Problems. Analyses of the data (correlational, factor analytic, and stepwise multiple regression) revealed a stable personality index representative of individuals exhibiting riskiness in decision-making. Variables contributing to this pattern characterized a dynamic task oriented individual. The results of this study should prove useful to future decision-making research by providing a framework for prediction.", "PMID": 957088}
{"id": "pubmed23n0032_2563", "title": "An evaluation of an MMPI response consistency measure.", "content": "Response inconsistency as an index for invalidating MMPI protocols was investigated by measuring the number of inconsistent responses given to 14 pairs of identical items by brain-damaged and nonbrain-damaged schizophrenic, alcoholic, neurotic and normal patients (n = 22 in each of the 8 groups). In addition the patient's number of inconsistent responses were correlated with the usual MMPI validity measures (?, L, F and K). Although all the response consistency scores of the groups were high it was found that the brain-damaged were more response inconsistent than the nonbrain-damaged patients (regardless of psychiatric diagnoses). The correlations between response inconsistency and the MMPI validity measures were significant but low. Because of the over-all high level of response consistency found for all the groups, it was concluded that MMPI protocol validity should be based on the validity scale measures, rather than upon response inconsistency.", "contents": "An evaluation of an MMPI response consistency measure. Response inconsistency as an index for invalidating MMPI protocols was investigated by measuring the number of inconsistent responses given to 14 pairs of identical items by brain-damaged and nonbrain-damaged schizophrenic, alcoholic, neurotic and normal patients (n = 22 in each of the 8 groups). In addition the patient's number of inconsistent responses were correlated with the usual MMPI validity measures (?, L, F and K). Although all the response consistency scores of the groups were high it was found that the brain-damaged were more response inconsistent than the nonbrain-damaged patients (regardless of psychiatric diagnoses). The correlations between response inconsistency and the MMPI validity measures were significant but low. Because of the over-all high level of response consistency found for all the groups, it was concluded that MMPI protocol validity should be based on the validity scale measures, rather than upon response inconsistency.", "PMID": 957089}
{"id": "pubmed23n0032_2564", "title": "An objective measure of fear of success: construction and validation.", "content": "A 27-item Fear of Success Scale (FOSS) was developed to assess individual differences in the motive to avoid success. Females scored significantly higher on the FOSS than did males; the FOSS was positively related to Horner's projective measure of fear of success, and negatively related to Mehrabian's measure of achievement motivation; subjects (both males and females) with high scores on the FOSS (a) performed less well on an anagram test, (b) attributed success more to external factors, and (c) attributed failure more to internal factors than subjects with low scores on the FOSS. These results supported the validity of the FOSS with regard to its use in future research.", "contents": "An objective measure of fear of success: construction and validation. A 27-item Fear of Success Scale (FOSS) was developed to assess individual differences in the motive to avoid success. Females scored significantly higher on the FOSS than did males; the FOSS was positively related to Horner's projective measure of fear of success, and negatively related to Mehrabian's measure of achievement motivation; subjects (both males and females) with high scores on the FOSS (a) performed less well on an anagram test, (b) attributed success more to external factors, and (c) attributed failure more to internal factors than subjects with low scores on the FOSS. These results supported the validity of the FOSS with regard to its use in future research.", "PMID": 957090}
{"id": "pubmed23n0032_2565", "title": "The Stanford Preschool Internal-External Scale: extension to kindergarteners.", "content": "To investigate whether the characteristics of the recently developed Stanford Preschool Internal-External Scale (SPIES; Mischel, Zeiss, & Zeiss, 1974) would remain stable for older children of apparently different socioeconomic status than the normative sample, the SPIES was administered to 104 kindergarteners of essentially middle class background in an ordinary elementary school. The findings indicate that the SPIES may be useful in research on perceived locus of control in kindergarteners as well as preschool children.", "contents": "The Stanford Preschool Internal-External Scale: extension to kindergarteners. To investigate whether the characteristics of the recently developed Stanford Preschool Internal-External Scale (SPIES; Mischel, Zeiss, & Zeiss, 1974) would remain stable for older children of apparently different socioeconomic status than the normative sample, the SPIES was administered to 104 kindergarteners of essentially middle class background in an ordinary elementary school. The findings indicate that the SPIES may be useful in research on perceived locus of control in kindergarteners as well as preschool children.", "PMID": 957091}
{"id": "pubmed23n0032_2566", "title": "Kinesthetic aftereffect and personality: a case study of issues involved in construct validation.", "content": "Kinesthetic Aftereffect (KAE), once a promising personality index, has been abandoned by many investigators because of poor retest reliability and intermittent validity. In challenging this current consensus, we argue that (a) first-session KAE is valid; (b) poor retest reliability simply reflects later-session bias; (c) hence, multisession studies should not be used to assess validity without taking this bias into account. Those recent studies which failed to support KAE validity were each multisession in design. If our bias contention is correct, these studies should be ignored, and the claim of intermittent validity is thus rebutted. Reanalysis of the most recent major multisession, nonsupportive validity study indicates (a) Session 1 validity, (b) later-session bias, and (c) later-session valdiity when multisession scores are combined to avoid bias. Thus, KAE validly measures personality.", "contents": "Kinesthetic aftereffect and personality: a case study of issues involved in construct validation. Kinesthetic Aftereffect (KAE), once a promising personality index, has been abandoned by many investigators because of poor retest reliability and intermittent validity. In challenging this current consensus, we argue that (a) first-session KAE is valid; (b) poor retest reliability simply reflects later-session bias; (c) hence, multisession studies should not be used to assess validity without taking this bias into account. Those recent studies which failed to support KAE validity were each multisession in design. If our bias contention is correct, these studies should be ignored, and the claim of intermittent validity is thus rebutted. Reanalysis of the most recent major multisession, nonsupportive validity study indicates (a) Session 1 validity, (b) later-session bias, and (c) later-session valdiity when multisession scores are combined to avoid bias. Thus, KAE validly measures personality.", "PMID": 957097}
{"id": "pubmed23n0032_2567", "title": "Stability of adult personality traits: fact or fable?", "content": "Theoretical positions for and against a stability model for adult personality traits are examined. Results of a sequential study of 19 personality factors over a 7-year period and with participants ranging in age from 22 to 84 years suggest that stability within generations appears to be the rule, albeit combined with many differences between successive population cohorts and subject to transient secular trends. A typology of 13 possible models is offered to account for the different developmental trends observed for the various personality factors.", "contents": "Stability of adult personality traits: fact or fable? Theoretical positions for and against a stability model for adult personality traits are examined. Results of a sequential study of 19 personality factors over a 7-year period and with participants ranging in age from 22 to 84 years suggest that stability within generations appears to be the rule, albeit combined with many differences between successive population cohorts and subject to transient secular trends. A typology of 13 possible models is offered to account for the different developmental trends observed for the various personality factors.", "PMID": 957098}
{"id": "pubmed23n0032_2568", "title": "The experience of crowding: a two-factor theory.", "content": "It was proposed that the experience of crowding occurs in a two-step process: First, the individual becomes aroused by violations of his personal space, and then he attributes the cause of this arousal to other people in his environment. Based on this model it was predicted that violations of personal space rather than simple density is the spatial variable associated with crowding. Further, it was predicted that the experience of crowding can be alleviated if the individual is distracted from making the attribution that his arousal is caused by other people. In order to test these prediction, interaction distance (close and far) and density (high and low) were varied separately. In addition, for half of the conditions, pictures (attribution inhibitors) were placed on the walls of the experimental room, and in the other half, the walls were bare. Subjects worked on two tasks and were then questioned about their experience of crowding. The results showed that interaction distance was more closely related to crowding than was density and that the addition of pictures reduced the experience of crowding only in the close interaction conditions. The results were interpreted as supporting the attribution analysis of crowding.", "contents": "The experience of crowding: a two-factor theory. It was proposed that the experience of crowding occurs in a two-step process: First, the individual becomes aroused by violations of his personal space, and then he attributes the cause of this arousal to other people in his environment. Based on this model it was predicted that violations of personal space rather than simple density is the spatial variable associated with crowding. Further, it was predicted that the experience of crowding can be alleviated if the individual is distracted from making the attribution that his arousal is caused by other people. In order to test these prediction, interaction distance (close and far) and density (high and low) were varied separately. In addition, for half of the conditions, pictures (attribution inhibitors) were placed on the walls of the experimental room, and in the other half, the walls were bare. Subjects worked on two tasks and were then questioned about their experience of crowding. The results showed that interaction distance was more closely related to crowding than was density and that the addition of pictures reduced the experience of crowding only in the close interaction conditions. The results were interpreted as supporting the attribution analysis of crowding.", "PMID": 957099}
{"id": "pubmed23n0032_2569", "title": "Role-taking standpoint and adolescent usage of Kohlberg's conventional stages of moral reasoning.", "content": "By taking into account a respondent's role-taking standpoint, this study proposes that moral judgments coded at Kohlberg's conventional stages can be expected to vary depending upon (a) the identity of the protagonist implicated in the moral dilemma and (b) the nature of the issues raised by the moral dilemmas used. Different groups of respondents were asked to answer three versions of Kohlberg's moral judgment questionnaire. One version implicated strangers in the dilemmas used (fictitious-other questionnaire), while the other two versions implicated respondents' best friends or mothers (primary-other questionnaires). A comparison of these questionnaire treatments indicates that Stage 3 response rate significantly increases and Stage 4 response rate significantly decreases when respondents answer primary-other questionnaires. It is suggested that this finding casts doubt on the adequacy of the displacement view of moral stage acquisition. The displacement perspective argues that as more cognitively complex structures are acquired, earlier acquired structures are displaced. In contrast, the present study proposes that a \"nondisplacement\" view of stage acquisition is required in order to explain variation observed at Stage 3 when it may be assumed that a Stage 4 reasoning capability has been acquired.", "contents": "Role-taking standpoint and adolescent usage of Kohlberg's conventional stages of moral reasoning. By taking into account a respondent's role-taking standpoint, this study proposes that moral judgments coded at Kohlberg's conventional stages can be expected to vary depending upon (a) the identity of the protagonist implicated in the moral dilemma and (b) the nature of the issues raised by the moral dilemmas used. Different groups of respondents were asked to answer three versions of Kohlberg's moral judgment questionnaire. One version implicated strangers in the dilemmas used (fictitious-other questionnaire), while the other two versions implicated respondents' best friends or mothers (primary-other questionnaires). A comparison of these questionnaire treatments indicates that Stage 3 response rate significantly increases and Stage 4 response rate significantly decreases when respondents answer primary-other questionnaires. It is suggested that this finding casts doubt on the adequacy of the displacement view of moral stage acquisition. The displacement perspective argues that as more cognitively complex structures are acquired, earlier acquired structures are displaced. In contrast, the present study proposes that a \"nondisplacement\" view of stage acquisition is required in order to explain variation observed at Stage 3 when it may be assumed that a Stage 4 reasoning capability has been acquired.", "PMID": 957100}
{"id": "pubmed23n0032_2570", "title": "Vicarious eyelid conditioning.", "content": "This study attempted to demonstrate that elicited electromyographic (EMG) reactions in observers may become conditioned to environmental events during the observation of a modeling sequence. Observers were shown a videotape of a model in a simulated direct eyelid discriminative conditioning situation involving adaptation, acquisition, and extinction trials. Various EMG measures were obtained in order to determine the occurrence of vicarious instigation and conditioning. The results demonstrated vicarious EMG eyelid responses. Also, the motor reactions were found to be localized to specific muscular activity rather than a result of general arousal. Some theoretical and clinical implications of these findings are discussed.", "contents": "Vicarious eyelid conditioning. This study attempted to demonstrate that elicited electromyographic (EMG) reactions in observers may become conditioned to environmental events during the observation of a modeling sequence. Observers were shown a videotape of a model in a simulated direct eyelid discriminative conditioning situation involving adaptation, acquisition, and extinction trials. Various EMG measures were obtained in order to determine the occurrence of vicarious instigation and conditioning. The results demonstrated vicarious EMG eyelid responses. Also, the motor reactions were found to be localized to specific muscular activity rather than a result of general arousal. Some theoretical and clinical implications of these findings are discussed.", "PMID": 957101}
{"id": "pubmed23n0032_2571", "title": "Nonlinear pharmacokinetic model of intravenous anesthesia.", "content": "A nonlinear pharmacokinetic model was constructed to describe the body distribution of intravenous anesthetics, using the physiological modeling approach for drug distribution kinetics. The model considers the physiological parameters of tissue volumes and blood flow rates for the standard four phases of blood, viscera, lean tissue, and adipose tissue and also the associated drug parameters known to influence drug distribution. A simple ramp function having a characteristic time (volume per flow rate) is used to describe the rate of approach to equilibrium conditions for each tissue phase. The model was evaluated for the distribution of methohexital and thiopental by comparing calculated values to experimental drug concentrations taken from the literature. The physiological alteration of obesity also was programmed into the model to evaluate its capability for predicting the influence of body alterations on drug distribution. The results indicated that a preliminary mathematical model of relatively simple design is capable of at least a semiquantitative prediction of intravenous anesthetic drug concentrations in body tissues and has the potential of accounting for differences in drug distribution in the presence of selected physiological alterations.", "contents": "Nonlinear pharmacokinetic model of intravenous anesthesia. A nonlinear pharmacokinetic model was constructed to describe the body distribution of intravenous anesthetics, using the physiological modeling approach for drug distribution kinetics. The model considers the physiological parameters of tissue volumes and blood flow rates for the standard four phases of blood, viscera, lean tissue, and adipose tissue and also the associated drug parameters known to influence drug distribution. A simple ramp function having a characteristic time (volume per flow rate) is used to describe the rate of approach to equilibrium conditions for each tissue phase. The model was evaluated for the distribution of methohexital and thiopental by comparing calculated values to experimental drug concentrations taken from the literature. The physiological alteration of obesity also was programmed into the model to evaluate its capability for predicting the influence of body alterations on drug distribution. The results indicated that a preliminary mathematical model of relatively simple design is capable of at least a semiquantitative prediction of intravenous anesthetic drug concentrations in body tissues and has the potential of accounting for differences in drug distribution in the presence of selected physiological alterations.", "PMID": 957106}
{"id": "pubmed23n0032_2572", "title": "Anticonvulsant activity and inhibition of cellular respiratory activity by substituted imidazolocarbamides.", "content": "Several 1-(1-aryl-2-mercaptoacetylimidazole)-3-alkylcarbamides were synthesized and characterized by their sharp melting points, elemental analyses, and IR spectra. These substituted imidazolocarbamides possessed anticonvulsant activity, which was reflected by the 20-80% protection observed with these compounds against pentylenetetrazol-induced convulsions in mice. These substituted imidazolocarbamides selectively inhibited the in vitro oxidation of nicotinamide adenine dinucleotide (NAD)-dependent oxidations of pyruvate, alpha-ketoglutarate, beta-hydroxybutyrate, and NADH by rat brain homogenates. However, NAD-independent oxidation of succinate was not affected. The anticonvulsant activity possessed by 1-(1-aryl-2-mercaptoacetylimidazole)-3-alkylcarbamides had no relationship to their ability to inhibit cellular respiratory activity.", "contents": "Anticonvulsant activity and inhibition of cellular respiratory activity by substituted imidazolocarbamides. Several 1-(1-aryl-2-mercaptoacetylimidazole)-3-alkylcarbamides were synthesized and characterized by their sharp melting points, elemental analyses, and IR spectra. These substituted imidazolocarbamides possessed anticonvulsant activity, which was reflected by the 20-80% protection observed with these compounds against pentylenetetrazol-induced convulsions in mice. These substituted imidazolocarbamides selectively inhibited the in vitro oxidation of nicotinamide adenine dinucleotide (NAD)-dependent oxidations of pyruvate, alpha-ketoglutarate, beta-hydroxybutyrate, and NADH by rat brain homogenates. However, NAD-independent oxidation of succinate was not affected. The anticonvulsant activity possessed by 1-(1-aryl-2-mercaptoacetylimidazole)-3-alkylcarbamides had no relationship to their ability to inhibit cellular respiratory activity.", "PMID": 957107}
{"id": "pubmed23n0032_2573", "title": "Quantum chemical studies on drug action V: Involvement of structure-activity, quantum chemical, and hydrophobicity factors in thrombocyte uptake of 5-hydroxytryptamine.", "content": "Inhibition of the uptake of 5-hydroxytryptamine (serotonin) in the thrombocyte by various tryptamine derivatives was investigated. The activity depended on the nature and position of the substituent. This activity was correlated with the total orbital energy and hydrophobicity factors. Other quantum parameters, such as the highest occupied molecular orbital energy and the lowest empty molecular orbital energy, failed to correlate. The possible involvement of two receptor sites that are sterically and electronically dissimilar is postulated because compounds fell into two distinct groups. The hydrophobicity factor was important in only one group of compounds, while the electronic factor was important in both.", "contents": "Quantum chemical studies on drug action V: Involvement of structure-activity, quantum chemical, and hydrophobicity factors in thrombocyte uptake of 5-hydroxytryptamine. Inhibition of the uptake of 5-hydroxytryptamine (serotonin) in the thrombocyte by various tryptamine derivatives was investigated. The activity depended on the nature and position of the substituent. This activity was correlated with the total orbital energy and hydrophobicity factors. Other quantum parameters, such as the highest occupied molecular orbital energy and the lowest empty molecular orbital energy, failed to correlate. The possible involvement of two receptor sites that are sterically and electronically dissimilar is postulated because compounds fell into two distinct groups. The hydrophobicity factor was important in only one group of compounds, while the electronic factor was important in both.", "PMID": 957108}
{"id": "pubmed23n0032_2574", "title": "Coumarins XIV: high-resolution mass spectra of 3',4'-disubstituted 3',4'-dihydroseselins.", "content": "High-resolution mass spectra of 14 3',4'-disubstituted 3',4'-dihydroseselins were examined. The nature of the substituents determinines the mode of fragmentation. Compounds having one or two acyloxy substituents fragment mainly by a pathway leading to the stable coumarinopyrilium ion. Coumarins with alkoxy or hydroxy substituents proceed by way of fission of the chroman ring, accompanied by the loss of two ring carbon atoms. Several generalizations are formulated which will aid in the interpretation of the mass spectra of this class of coumarins from a structural standpoint.", "contents": "Coumarins XIV: high-resolution mass spectra of 3',4'-disubstituted 3',4'-dihydroseselins. High-resolution mass spectra of 14 3',4'-disubstituted 3',4'-dihydroseselins were examined. The nature of the substituents determinines the mode of fragmentation. Compounds having one or two acyloxy substituents fragment mainly by a pathway leading to the stable coumarinopyrilium ion. Coumarins with alkoxy or hydroxy substituents proceed by way of fission of the chroman ring, accompanied by the loss of two ring carbon atoms. Several generalizations are formulated which will aid in the interpretation of the mass spectra of this class of coumarins from a structural standpoint.", "PMID": 957109}
{"id": "pubmed23n0032_2575", "title": "Pharmacokinetic studies of pentylenetetrazol in dogs.", "content": "Pharmacokinetic profiles of pentylenetetrazol in the dog were studied following rapid intravenous and oral administrations of a convulsant dose (15-20 mg/kg). Plasma level-time curves after a rapid intravenous injection showed biexponential decline, indicating that the disposition of this drug in the dog follows a two-compartment body model. Pharmacokinetic parameters were calculated from the intravenous data. After oral administration of the solution dose, the peak plasma level appeared at about 30 min postdose, indicating that the absorption occurs rapidly. Areas under the oral plasma level-time curves s howed that the drug was absorbed completely and that the first-pass metabolism effect was minimal. The ligation studies of the kidney and the liver suggested that the main elimination pathway of this drug was biotransformation in the liver. The average plasma half-life was 1.4 hr. At steady state, the volume of distribution was approximately equivalent to the volume of the total body water.", "contents": "Pharmacokinetic studies of pentylenetetrazol in dogs. Pharmacokinetic profiles of pentylenetetrazol in the dog were studied following rapid intravenous and oral administrations of a convulsant dose (15-20 mg/kg). Plasma level-time curves after a rapid intravenous injection showed biexponential decline, indicating that the disposition of this drug in the dog follows a two-compartment body model. Pharmacokinetic parameters were calculated from the intravenous data. After oral administration of the solution dose, the peak plasma level appeared at about 30 min postdose, indicating that the absorption occurs rapidly. Areas under the oral plasma level-time curves s howed that the drug was absorbed completely and that the first-pass metabolism effect was minimal. The ligation studies of the kidney and the liver suggested that the main elimination pathway of this drug was biotransformation in the liver. The average plasma half-life was 1.4 hr. At steady state, the volume of distribution was approximately equivalent to the volume of the total body water.", "PMID": 957110}
{"id": "pubmed23n0032_2576", "title": "Model systems for dissolution of finely divided (multisized) drug powders.", "content": "Several reports have dealt with model systems for the dissolution of log-normally distributed powders. A numerical solution is presented for the previously published Higuchi-Hiestand equations for a log-normal particle-size distribution. This solution was realized via an application of the System 360 Continuous System Modeling Program (CSMP). The resulting computer-aided calculations were utilized in the comparisons between the Higuchi-Hiestand model and other existing models. These comparisons provided a basis for the development of an adaptation of the Nielsen moving sphere model for log-normally distributed powders. The adaptation of the Nielsen equation for multisized particle systems is suggested as being potentially useful for treating experimental data where hydrodynamic effects must be considered.", "contents": "Model systems for dissolution of finely divided (multisized) drug powders. Several reports have dealt with model systems for the dissolution of log-normally distributed powders. A numerical solution is presented for the previously published Higuchi-Hiestand equations for a log-normal particle-size distribution. This solution was realized via an application of the System 360 Continuous System Modeling Program (CSMP). The resulting computer-aided calculations were utilized in the comparisons between the Higuchi-Hiestand model and other existing models. These comparisons provided a basis for the development of an adaptation of the Nielsen moving sphere model for log-normally distributed powders. The adaptation of the Nielsen equation for multisized particle systems is suggested as being potentially useful for treating experimental data where hydrodynamic effects must be considered.", "PMID": 957111}
{"id": "pubmed23n0032_2577", "title": "Binding characteristics of drugs to synthetic levodopa melanin.", "content": "To define binding characteristics of drugs, levodopa melanin was prepared with the aid of mushroom tyrosinase. The binding of radiolabeled substances was studied with increasing concentrations of melanin in a fixed volume of potassium phosphate buffer (pH 7.4) at 37 degrees. The affinity and capacity of the drug binding were calculated according to Langmuir's adsorption isotherm. The affinity constant of various sympathomimetic amines such as (-)-amphetamine, (+)-amphetamine, (-)-ephedrine, (+/-)-octopamine, and (+)-norepinephrine ranged from 1.1 to 2.8 X 10(5) M-1. The binding capacity for the amines ranged from 1.4 to 3.2 X 10(-9) mole/mg. Although the capacity of (+/-)-cocaine for binding was similar to that of the amines, the affinity was slightly higher, 8.9 X 10(5) M-1. The binding of atropine to the synthetic melanin appeared to be a saturable process with the affinity and capacity values of 0.2 X 10(5) M-1 and 7.6 X 10(-9) mole/mg, respectively. Although the binding lacks stereoselectivity, the drugs vary in their capacity and affinity to bind with melanin. The observed differential pharmacological and toxicologic properties of drugs in the pigmented tissues may in part be related to their differential bi binding characteristics.", "contents": "Binding characteristics of drugs to synthetic levodopa melanin. To define binding characteristics of drugs, levodopa melanin was prepared with the aid of mushroom tyrosinase. The binding of radiolabeled substances was studied with increasing concentrations of melanin in a fixed volume of potassium phosphate buffer (pH 7.4) at 37 degrees. The affinity and capacity of the drug binding were calculated according to Langmuir's adsorption isotherm. The affinity constant of various sympathomimetic amines such as (-)-amphetamine, (+)-amphetamine, (-)-ephedrine, (+/-)-octopamine, and (+)-norepinephrine ranged from 1.1 to 2.8 X 10(5) M-1. The binding capacity for the amines ranged from 1.4 to 3.2 X 10(-9) mole/mg. Although the capacity of (+/-)-cocaine for binding was similar to that of the amines, the affinity was slightly higher, 8.9 X 10(5) M-1. The binding of atropine to the synthetic melanin appeared to be a saturable process with the affinity and capacity values of 0.2 X 10(5) M-1 and 7.6 X 10(-9) mole/mg, respectively. Although the binding lacks stereoselectivity, the drugs vary in their capacity and affinity to bind with melanin. The observed differential pharmacological and toxicologic properties of drugs in the pigmented tissues may in part be related to their differential bi binding characteristics.", "PMID": 957112}
{"id": "pubmed23n0032_2578", "title": "Hermetic packaging of drugs: optimized sealing of foil pouches.", "content": "Factors affecting the sealing of foil packages were studied in the sealing of foil packages were studied in three laboratories. The relationship of sealing temperature (with machine speed and pressure kept constant) to the incidence of defective packages was determined. The maximum acceptable limit for defective pouches was 1%. Three tests were employed to detect defects: vacuum-dye, seal strength, and pressurized ammonia vapor. Only the last was sensitive enough to determine the optimum sealing conditions. This test also was capable of detecting leakage sites. Replacement of the cellophane layer of the foil laminate with polyvinylidene chloride-coated polyester improved the barrier properties of the package.", "contents": "Hermetic packaging of drugs: optimized sealing of foil pouches. Factors affecting the sealing of foil packages were studied in the sealing of foil packages were studied in three laboratories. The relationship of sealing temperature (with machine speed and pressure kept constant) to the incidence of defective packages was determined. The maximum acceptable limit for defective pouches was 1%. Three tests were employed to detect defects: vacuum-dye, seal strength, and pressurized ammonia vapor. Only the last was sensitive enough to determine the optimum sealing conditions. This test also was capable of detecting leakage sites. Replacement of the cellophane layer of the foil laminate with polyvinylidene chloride-coated polyester improved the barrier properties of the package.", "PMID": 957113}
{"id": "pubmed23n0032_2579", "title": "Comparison of observed and predicted first-pass metabolism of imipramine in humans.", "content": "The first-pass metabolism of imipramine was calculated based on the dose, hepatic blood flow, and total area under the plasma-time curve after oral administration of 0.71 +/- 0.03 mg/kg of imipramine to four individuals suffering from mild depression. The predicted values of first-pass metabolism ranged from 37 to 68%, consistent with experimentally derived estimates.", "contents": "Comparison of observed and predicted first-pass metabolism of imipramine in humans. The first-pass metabolism of imipramine was calculated based on the dose, hepatic blood flow, and total area under the plasma-time curve after oral administration of 0.71 +/- 0.03 mg/kg of imipramine to four individuals suffering from mild depression. The predicted values of first-pass metabolism ranged from 37 to 68%, consistent with experimentally derived estimates.", "PMID": 957114}
{"id": "pubmed23n0032_2580", "title": "High-pressure liquid chromatographic determination of acetaminophen in biological fluids.", "content": "A method for the rapid estimation of free acetaminophen in biological fluids is described. The assay involves either extraction and high-pressure liquid chromatographic analysis on a 10-mum particle-size silica gel column, using a mobile phase of 10% chloroform in tetrahydrofuran. The procedure was used to determine acetaminophen levels in urine from two healthy volunteers who ingested 650 mg of 14C-acetaminophen (20 muCi), and the accuracy of the method was compared with the carbon-14 determination. The limit of detectability for acetaminophen is 1 mug/ml.", "contents": "High-pressure liquid chromatographic determination of acetaminophen in biological fluids. A method for the rapid estimation of free acetaminophen in biological fluids is described. The assay involves either extraction and high-pressure liquid chromatographic analysis on a 10-mum particle-size silica gel column, using a mobile phase of 10% chloroform in tetrahydrofuran. The procedure was used to determine acetaminophen levels in urine from two healthy volunteers who ingested 650 mg of 14C-acetaminophen (20 muCi), and the accuracy of the method was compared with the carbon-14 determination. The limit of detectability for acetaminophen is 1 mug/ml.", "PMID": 957115}
{"id": "pubmed23n0032_2581", "title": "GLC determination of doxepin plasma levels.", "content": "A procedure is described for extracting doxepin, a tricyclic antidepressant, from plasma and subsequently measuring its concentration by GLC. The developed technique permits the resolution and quantitative determination of the cis- and trans-isomers of doxepin as well as its desmethyl metabolite. The method allows precise, reliable measurement of the drug and one of its metabolities in concentrations as low as 10 ng/ml of plasma.", "contents": "GLC determination of doxepin plasma levels. A procedure is described for extracting doxepin, a tricyclic antidepressant, from plasma and subsequently measuring its concentration by GLC. The developed technique permits the resolution and quantitative determination of the cis- and trans-isomers of doxepin as well as its desmethyl metabolite. The method allows precise, reliable measurement of the drug and one of its metabolities in concentrations as low as 10 ng/ml of plasma.", "PMID": 957116}
{"id": "pubmed23n0032_2582", "title": "Calibration of a horizontal pendulum-type tablet breaking strength tester.", "content": "A rapid, simplified method of checking the calibration of a new motorized pendulum type of tablet hardness tester and the accuracy and precision of three testers were determined. Two force gauges were calibrated, and both gauges were used by two operators to determine the accuracy of the new testers. The calibration method using the force gauge was reliable for checking the accuracy. The results show the various new testers to be in calibration and to give reproducible results.", "contents": "Calibration of a horizontal pendulum-type tablet breaking strength tester. A rapid, simplified method of checking the calibration of a new motorized pendulum type of tablet hardness tester and the accuracy and precision of three testers were determined. Two force gauges were calibrated, and both gauges were used by two operators to determine the accuracy of the new testers. The calibration method using the force gauge was reliable for checking the accuracy. The results show the various new testers to be in calibration and to give reproducible results.", "PMID": 957117}
{"id": "pubmed23n0032_2583", "title": "Antimicrobial agents: synthesis and antimicrobial activity of new aryloxyalkyl esters of p-hydroxybenzoic acid.", "content": "Several new aryloxyalkyl esters of p-hydroxybenzoic acid were synthesized and screened for in vitro antimicrobial activity. Although a few compounds showed low antifungal activity, many possessed appreciable in vitro antibacterial activity. However, none of these compounds was active against Mycobacterium tuberculosis (H37Rv).", "contents": "Antimicrobial agents: synthesis and antimicrobial activity of new aryloxyalkyl esters of p-hydroxybenzoic acid. Several new aryloxyalkyl esters of p-hydroxybenzoic acid were synthesized and screened for in vitro antimicrobial activity. Although a few compounds showed low antifungal activity, many possessed appreciable in vitro antibacterial activity. However, none of these compounds was active against Mycobacterium tuberculosis (H37Rv).", "PMID": 957118}
{"id": "pubmed23n0032_2584", "title": "Anti-inflammatory and antiproteolytic properties of 1-(1-naphthylacetyl)-3-substituted carbamides.", "content": "Several 1-(1-naphthylacetyl)-3-substituted carbamides were synthesized, characterized, and evaluated for anti-inflammatory and antiproteolytic activity. The protection afforded by most of these carbamides against carrageenan-induced edema in rats at a dose of 100 mg/kg ranged from 4.4 to 50%. Some of these carbamides, which showed higher protection against carrageenan-induced edema, were further evaluated for their antigranulation effect against cotton pellet-induced granuloma formation in rats. All carbamides showed a poor degree of protection against granuloma formation. The antiproteolytic activity of these carbamides, as reflected by their ability to inhibit trypsin-induced hydrolysis of the bovine serum albumin, was of a low order and was unrelated to their anti-inflammatory activity.", "contents": "Anti-inflammatory and antiproteolytic properties of 1-(1-naphthylacetyl)-3-substituted carbamides. Several 1-(1-naphthylacetyl)-3-substituted carbamides were synthesized, characterized, and evaluated for anti-inflammatory and antiproteolytic activity. The protection afforded by most of these carbamides against carrageenan-induced edema in rats at a dose of 100 mg/kg ranged from 4.4 to 50%. Some of these carbamides, which showed higher protection against carrageenan-induced edema, were further evaluated for their antigranulation effect against cotton pellet-induced granuloma formation in rats. All carbamides showed a poor degree of protection against granuloma formation. The antiproteolytic activity of these carbamides, as reflected by their ability to inhibit trypsin-induced hydrolysis of the bovine serum albumin, was of a low order and was unrelated to their anti-inflammatory activity.", "PMID": 957119}
{"id": "pubmed23n0032_2585", "title": "Isolation of cannabisativine, an alkaloid, from Cannabis sativa L. root.", "content": "An ethanol extract of the root of a Mexican variant of Cannabis sativa L.(marijuana) afforded, after partitioning and chromatography, the new spermidine alkaloid cannabisativine.", "contents": "Isolation of cannabisativine, an alkaloid, from Cannabis sativa L. root. An ethanol extract of the root of a Mexican variant of Cannabis sativa L.(marijuana) afforded, after partitioning and chromatography, the new spermidine alkaloid cannabisativine.", "PMID": 957120}
{"id": "pubmed23n0032_2586", "title": "Synthesis and antibacterial and antifungal activities of alkyl and polyhalophenyl esters of benzo[b]-3-methyl-2-furancarbamic acid.", "content": "Several alkyl and polyhalophenyl esters of benzo[b]-3-methyl-2-furancarbamic acid were prepared and tested for antifungal activity against Candida albicans, Penicillium notatum, and Aspergillus niger. The pentachlorophenyl ester was the most active substance and the only compound to show antibacterial activity against Staphylococcus aureus.", "contents": "Synthesis and antibacterial and antifungal activities of alkyl and polyhalophenyl esters of benzo[b]-3-methyl-2-furancarbamic acid. Several alkyl and polyhalophenyl esters of benzo[b]-3-methyl-2-furancarbamic acid were prepared and tested for antifungal activity against Candida albicans, Penicillium notatum, and Aspergillus niger. The pentachlorophenyl ester was the most active substance and the only compound to show antibacterial activity against Staphylococcus aureus.", "PMID": 957121}
{"id": "pubmed23n0032_2587", "title": "Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high-pressure liquid chromatography: reversed-phase, ion-pairing approach.", "content": "The reversed-phase, ion-pairing approach to high-pressure liquid chromatography was applied to the simultaneous analysis of hydrocortisone and its phosphate ester in laboratory-prepared samples and injectable solutions. Results of this technique were evaluated and compared with results of the official procedure.", "contents": "Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high-pressure liquid chromatography: reversed-phase, ion-pairing approach. The reversed-phase, ion-pairing approach to high-pressure liquid chromatography was applied to the simultaneous analysis of hydrocortisone and its phosphate ester in laboratory-prepared samples and injectable solutions. Results of this technique were evaluated and compared with results of the official procedure.", "PMID": 957122}
{"id": "pubmed23n0032_2588", "title": "High-pressure liquid chromatographic determination of thiothixene in pharmaceutical formulations.", "content": "A method was developed for the quantitative determination of thiothixene in pharmaceutical formulations by high-pressure liquid chromatography after dissolution of the sample in methanol.", "contents": "High-pressure liquid chromatographic determination of thiothixene in pharmaceutical formulations. A method was developed for the quantitative determination of thiothixene in pharmaceutical formulations by high-pressure liquid chromatography after dissolution of the sample in methanol.", "PMID": 957123}
{"id": "pubmed23n0032_2589", "title": "Structural features and protective activity of dexamethasone and pregnenolone-16 alpha-carbonitrile.", "content": "Pretreatment with dexamethasone acetate or pregnenolone-16 alpha-carbonitrile markedly diminished the pharmacological effect of zoxazolamine in rats. This prophylactic action was associated with significantly decreased plasma drug levels, which, in turn, were correlated with enhanced hepatic drug biotransformation, induced by the steroids. Dexamethasone proved to be more active than pregnenolone-16 alpha-carbonitrile in this respect. The A-ring conformation as well as the distances of O-3-O-11, O-11-O-17, and the O-3-mean plane C-5-C-17 may be key factors in glucocorticoid activity, and the longer the distances, the greater the potency. These characteristics have no bearing on catatoxic activity for which the 16 alpha-substituent appears to be a structural prerequisite.", "contents": "Structural features and protective activity of dexamethasone and pregnenolone-16 alpha-carbonitrile. Pretreatment with dexamethasone acetate or pregnenolone-16 alpha-carbonitrile markedly diminished the pharmacological effect of zoxazolamine in rats. This prophylactic action was associated with significantly decreased plasma drug levels, which, in turn, were correlated with enhanced hepatic drug biotransformation, induced by the steroids. Dexamethasone proved to be more active than pregnenolone-16 alpha-carbonitrile in this respect. The A-ring conformation as well as the distances of O-3-O-11, O-11-O-17, and the O-3-mean plane C-5-C-17 may be key factors in glucocorticoid activity, and the longer the distances, the greater the potency. These characteristics have no bearing on catatoxic activity for which the 16 alpha-substituent appears to be a structural prerequisite.", "PMID": 957124}
{"id": "pubmed23n0032_2590", "title": "Stability of vitamin C (ascorbic acid) in tablets.", "content": "Stability of ascorbic acid (vitamin C) in various tablet formulations and the nature and extent of formation of decomposition products (dehydroascorbic acid, diketogulonic acid, and oxalic acid) were determined under normal conditions of storage and in simulated use tests. IR spectrophotometric, colorimetric, fluorometric, titrimetric, polarographic, and chromatographic methods were applied. Recent implications concerning the instability of ascorbic acid in tablets and the potentially harmful nature of the breakdown products are shown to be unfounded. Under normal storage conditions, commerical-type ascorbic acid tablets are stable for over 5 years (greater than 95% potency retention). The amounts of all three breakdown products formed under the various storage conditions constitute a small percentage of the ascorbic acid content and pose no dietary hazard. IR spectroscopy was inadequate as a quantitative method for evaluating ascorbic acid potency in tablet formulations. The official titration methods and TLC, colorimetric, and polarographic determinations correlate well and define accurately the stability of ascorbic acid in these dosage forms.", "contents": "Stability of vitamin C (ascorbic acid) in tablets. Stability of ascorbic acid (vitamin C) in various tablet formulations and the nature and extent of formation of decomposition products (dehydroascorbic acid, diketogulonic acid, and oxalic acid) were determined under normal conditions of storage and in simulated use tests. IR spectrophotometric, colorimetric, fluorometric, titrimetric, polarographic, and chromatographic methods were applied. Recent implications concerning the instability of ascorbic acid in tablets and the potentially harmful nature of the breakdown products are shown to be unfounded. Under normal storage conditions, commerical-type ascorbic acid tablets are stable for over 5 years (greater than 95% potency retention). The amounts of all three breakdown products formed under the various storage conditions constitute a small percentage of the ascorbic acid content and pose no dietary hazard. IR spectroscopy was inadequate as a quantitative method for evaluating ascorbic acid potency in tablet formulations. The official titration methods and TLC, colorimetric, and polarographic determinations correlate well and define accurately the stability of ascorbic acid in these dosage forms.", "PMID": 957128}
{"id": "pubmed23n0032_2591", "title": "Combined assays for vitamins A, D (ergocalciferol), and E in multivitamin preparations with separation by reversed-phase partition chromatography.", "content": "A rapid method was developed whereby vitamins A, D (ergocalciferol), and E in multivitamin products are measured in a common assay sample. The method depends on reversed-phase partition chromatography by which the vitamin alcohols are eluted in separate fractions from a column. Vitamins A and E are then determined by their UV absorption, while vitamin D is measured colorimetrically with an antimony trichloride reagent. The column consists of diatomaceous earth impregnated with dimethyl polysiloxane, with n-heptane as the immobile solvent, and 90% methanol followed by 95% methanol as the mobile solvents. Vitamins A and D elute in that order in the 90% methanol, and finally vitamin E elutes in the 95% methanol fraction. The method is widely applicable to various types of multivitamin and vitamin-mineral products including oil-based, water-based, and dry formulations.", "contents": "Combined assays for vitamins A, D (ergocalciferol), and E in multivitamin preparations with separation by reversed-phase partition chromatography. A rapid method was developed whereby vitamins A, D (ergocalciferol), and E in multivitamin products are measured in a common assay sample. The method depends on reversed-phase partition chromatography by which the vitamin alcohols are eluted in separate fractions from a column. Vitamins A and E are then determined by their UV absorption, while vitamin D is measured colorimetrically with an antimony trichloride reagent. The column consists of diatomaceous earth impregnated with dimethyl polysiloxane, with n-heptane as the immobile solvent, and 90% methanol followed by 95% methanol as the mobile solvents. Vitamins A and D elute in that order in the 90% methanol, and finally vitamin E elutes in the 95% methanol fraction. The method is widely applicable to various types of multivitamin and vitamin-mineral products including oil-based, water-based, and dry formulations.", "PMID": 957129}
{"id": "pubmed23n0032_2592", "title": "Effect of inorganic additives on solutions of nonionic surfactants III: CMC's and surface properties.", "content": "Continuing work on the interaction of inorganic additives with nonionic surfactants in aqueous solution dealt with their effect on the CMC and surface tension. The surfactants were octoxynol and polyoxyethylated oleyl alcohol, containing an average of 9.5 and 10 ethylene oxide units, respectively. Their CMC values were lowered by most electrolytes studied, representing salting out of the surfactants. The steepest reductions in the CMC were produced by the nitrates of sodium and potassium, which had been found to lower the cloud points of nonionic surfactants, salting them out because of the inability of their cations to form complexes with the ether oxygen linkages of the polyoxyethylene moieties. However, even electrolytes with cations such as hydrogen, lithium, calcium, nickel, lead, and aluminum capable of forming complexes with the ether oxygens, thereby increasing the cloud points of the surfactants, lowered their CMC values. In the presence of increasing concentrations of the latter electrolytes, the CMC values frequently went through minima and approached the CMC of the surfactant in the absence of additives. Increases in the CMC over the entire range of additive concentrations investigated were produced by cadmium nitrate for octoxynol, urea for polyoxyethylated oleyl alcohol, and magnesium nitrate for both. Net increases in the plateau or micellar surface tension of polyoxyethylated oleyl alcohol, i.e., in the constant surface tension of surfactant solutions above the CMC, were brought about by the nitrates of cadmium, aluminum, and magnesium at low concentrations only and by urea at all concentrations. This increase is interpreted as salting in. The area per surfactant molecule adsorbed at the air-water interface was reduced by all added electrolytes. Urea caused no such reduction.", "contents": "Effect of inorganic additives on solutions of nonionic surfactants III: CMC's and surface properties. Continuing work on the interaction of inorganic additives with nonionic surfactants in aqueous solution dealt with their effect on the CMC and surface tension. The surfactants were octoxynol and polyoxyethylated oleyl alcohol, containing an average of 9.5 and 10 ethylene oxide units, respectively. Their CMC values were lowered by most electrolytes studied, representing salting out of the surfactants. The steepest reductions in the CMC were produced by the nitrates of sodium and potassium, which had been found to lower the cloud points of nonionic surfactants, salting them out because of the inability of their cations to form complexes with the ether oxygen linkages of the polyoxyethylene moieties. However, even electrolytes with cations such as hydrogen, lithium, calcium, nickel, lead, and aluminum capable of forming complexes with the ether oxygens, thereby increasing the cloud points of the surfactants, lowered their CMC values. In the presence of increasing concentrations of the latter electrolytes, the CMC values frequently went through minima and approached the CMC of the surfactant in the absence of additives. Increases in the CMC over the entire range of additive concentrations investigated were produced by cadmium nitrate for octoxynol, urea for polyoxyethylated oleyl alcohol, and magnesium nitrate for both. Net increases in the plateau or micellar surface tension of polyoxyethylated oleyl alcohol, i.e., in the constant surface tension of surfactant solutions above the CMC, were brought about by the nitrates of cadmium, aluminum, and magnesium at low concentrations only and by urea at all concentrations. This increase is interpreted as salting in. The area per surfactant molecule adsorbed at the air-water interface was reduced by all added electrolytes. Urea caused no such reduction.", "PMID": 957130}
{"id": "pubmed23n0032_2593", "title": "Effect of inorganic additives on solutions of nonionic surfactants IV: Krafft points.", "content": "Two polydisperse polyoxyethylated nonionic surfactants were found to possess Krafft points as well as cloud points. The significance of the Krafft point and the reason why it is rarely observed in nonionic surfactants are discussed. The values of the Krafft and cloud points of the two surfactants underwent only small changes as a function of surfactant concentration in the range of 0.5-7.5% (w/w). All electrolytes investigated, as well as urea, raised the Krafft points by between 1 and 4 degrees in the concentration range of 0.5-4 molal. Included were salting-in electrolytes, which raised the cloud points, as well as salting-out electrolytes, which lowered them. Electrolytes that salt out nonionic surfactants strongly may depress the cloud point to the Krafft point temperature, rendering a water-soluble surfactant insoluble in salt solutions at all temperatures.", "contents": "Effect of inorganic additives on solutions of nonionic surfactants IV: Krafft points. Two polydisperse polyoxyethylated nonionic surfactants were found to possess Krafft points as well as cloud points. The significance of the Krafft point and the reason why it is rarely observed in nonionic surfactants are discussed. The values of the Krafft and cloud points of the two surfactants underwent only small changes as a function of surfactant concentration in the range of 0.5-7.5% (w/w). All electrolytes investigated, as well as urea, raised the Krafft points by between 1 and 4 degrees in the concentration range of 0.5-4 molal. Included were salting-in electrolytes, which raised the cloud points, as well as salting-out electrolytes, which lowered them. Electrolytes that salt out nonionic surfactants strongly may depress the cloud point to the Krafft point temperature, rendering a water-soluble surfactant insoluble in salt solutions at all temperatures.", "PMID": 957131}
{"id": "pubmed23n0032_2594", "title": "Interactions of adenine, thymine, and uracil with epinephrine: UV studies.", "content": "Based upon UV absorption studies, charge transfer complex formations by the nucleic acid bases adenine, thymine, and uracil with epinephrine were demonstrated. The pertient equilibrium constants were calculated by assuming 1:1 complexes using the Benesi-Hildebrand equation and were found to decrease in the following order: uracil-epinephrine greater than thymine-epinephrine greater than adenine-epinephrine. The values of delta G degrees, delta H degrees, and delta S degrees were calculated for the various interactions. Theoretical arguments are presented as to the possible stoichiometries of the various complexes existing in aqueous solutions. The electron-donating or electron-accepting abilities of the interacting molecular species are discussed.", "contents": "Interactions of adenine, thymine, and uracil with epinephrine: UV studies. Based upon UV absorption studies, charge transfer complex formations by the nucleic acid bases adenine, thymine, and uracil with epinephrine were demonstrated. The pertient equilibrium constants were calculated by assuming 1:1 complexes using the Benesi-Hildebrand equation and were found to decrease in the following order: uracil-epinephrine greater than thymine-epinephrine greater than adenine-epinephrine. The values of delta G degrees, delta H degrees, and delta S degrees were calculated for the various interactions. Theoretical arguments are presented as to the possible stoichiometries of the various complexes existing in aqueous solutions. The electron-donating or electron-accepting abilities of the interacting molecular species are discussed.", "PMID": 957132}
{"id": "pubmed23n0032_2595", "title": "Analysis of alkaloid mixtures by charge-transfer complexation.", "content": "Binary mixtures of weak and strong UV-absorbing alkaloids were analyzed by a charge-transfer spectrophotometric method, utilizing iodine in ethylene dichloride as the acceptor. In the uncomplexed form, the strong absorbing alkaloid (papaverine, quinine, ergotamine, or reserpine) was measured at a wavelength where there was no interference from weak absorbers (at 335, 332, 315, or 300 nm, respectively). The weak absorbing alkaloid (ephedrine, codeine, atropine, or homatropine methylbromide) was determined by computing its contribution to the total charge-transfer band at 295 nm where absorbance was linearly additive for mixtures. The greater increase in the original epsilon-values of the weak absorbers upon complexation with iodine relative to the corresponding increase in the epsilon-values of the strong absorbers led to good recoveries even at the low dose ratios of the weakly absorbing, and often more potent, alkaloids.", "contents": "Analysis of alkaloid mixtures by charge-transfer complexation. Binary mixtures of weak and strong UV-absorbing alkaloids were analyzed by a charge-transfer spectrophotometric method, utilizing iodine in ethylene dichloride as the acceptor. In the uncomplexed form, the strong absorbing alkaloid (papaverine, quinine, ergotamine, or reserpine) was measured at a wavelength where there was no interference from weak absorbers (at 335, 332, 315, or 300 nm, respectively). The weak absorbing alkaloid (ephedrine, codeine, atropine, or homatropine methylbromide) was determined by computing its contribution to the total charge-transfer band at 295 nm where absorbance was linearly additive for mixtures. The greater increase in the original epsilon-values of the weak absorbers upon complexation with iodine relative to the corresponding increase in the epsilon-values of the strong absorbers led to good recoveries even at the low dose ratios of the weakly absorbing, and often more potent, alkaloids.", "PMID": 957133}
{"id": "pubmed23n0032_2596", "title": "Physical aspects of wet granulations I: distribution kinetics of water.", "content": "The kinetics of wetting during the process of making tablet granulations were studied; the process progresses through an intermediate (overwetted) nonequilibrium granule to a final, equilibrated granule. In systematic formulation changes, there is an optimum composition from the point of view of hardness and content of equilibrium granules.", "contents": "Physical aspects of wet granulations I: distribution kinetics of water. The kinetics of wetting during the process of making tablet granulations were studied; the process progresses through an intermediate (overwetted) nonequilibrium granule to a final, equilibrated granule. In systematic formulation changes, there is an optimum composition from the point of view of hardness and content of equilibrium granules.", "PMID": 957134}
{"id": "pubmed23n0032_2597", "title": "Comparative serum prednisone and prednisolone concentrations following prednisone or prednisolone administration to beagle dogs.", "content": "The relative serum prednisone and prednisolone concentrations were determined following the administration of prednisone or prednisolone as 5-mg tablets to male beagle dogs. Serum prednisone concentrations were significantly greater following prednisone administration than they were following prednisolone administration. Serum prednisolone concentrations were significantly greater following treatment with prednisolone than with prednisone. The combined prednisone and prednisolone areas under the serum concentration-time curves were similar for the two treatments.", "contents": "Comparative serum prednisone and prednisolone concentrations following prednisone or prednisolone administration to beagle dogs. The relative serum prednisone and prednisolone concentrations were determined following the administration of prednisone or prednisolone as 5-mg tablets to male beagle dogs. Serum prednisone concentrations were significantly greater following prednisone administration than they were following prednisolone administration. Serum prednisolone concentrations were significantly greater following treatment with prednisolone than with prednisone. The combined prednisone and prednisolone areas under the serum concentration-time curves were similar for the two treatments.", "PMID": 957135}
{"id": "pubmed23n0032_2598", "title": "Rectification in the smooth muscle cell membrane of rabbit aorta.", "content": "1. The current-voltage relation of the smooth muscle cell membrane of rabbit aorta was determined by the partition method. 2. No anomalous rectification was observed in any of the following solutions: normal Krebs, Na free choline, Na sulphate, and high K-Na free sulphate. 3. Delayed rectification was seen on application of depolarizing current in both normal Krebs solution and Na free choline solution. 4. High concentration of K made the steady-state current-voltage relation almost linear in a voltage range of about 0 to -20mV. This effect, and steady-state cathodal rectification which was seen in physiological solution, could be explained qualitatively by constant field theory without involving channels capable of anomalous rectification. 5. A slow decrease in K conductance, during application of large and long-lasting hyperpolarizing currents, which occurs in skeletal muscle and is attributed to the tubule system, was never observed in the arteries either in Krebs, Na-free choline, or Na sulphate solution.", "contents": "Rectification in the smooth muscle cell membrane of rabbit aorta. 1. The current-voltage relation of the smooth muscle cell membrane of rabbit aorta was determined by the partition method. 2. No anomalous rectification was observed in any of the following solutions: normal Krebs, Na free choline, Na sulphate, and high K-Na free sulphate. 3. Delayed rectification was seen on application of depolarizing current in both normal Krebs solution and Na free choline solution. 4. High concentration of K made the steady-state current-voltage relation almost linear in a voltage range of about 0 to -20mV. This effect, and steady-state cathodal rectification which was seen in physiological solution, could be explained qualitatively by constant field theory without involving channels capable of anomalous rectification. 5. A slow decrease in K conductance, during application of large and long-lasting hyperpolarizing currents, which occurs in skeletal muscle and is attributed to the tubule system, was never observed in the arteries either in Krebs, Na-free choline, or Na sulphate solution.", "PMID": 957157}
{"id": "pubmed23n0032_2599", "title": "A quantitative description of end-plate currents in the presence of two lidocaine derivatives.", "content": "1. Possible ways in which local anaesthetics may act to produce their characteristic alterations of end-plate current (e.p.c.s) are considered. 2. Following Magleby & Stevens (1972b), it is proposed that when acetylcholine (ACh) binds to its end-plate receptor it induces a conformational change which causes an increased ionic permeability and, thus, the measured e.p.c.; the reverse conformational change which returns the permeability to its resting state is postulated to be the rate-limiting event for e.p.c. decay whether or not local anaesthetics are present. Moreover, it is proposed that the ACh receptor had multiple binding sites and the local anaesthetics can bind to one or more of these sites thereby altering the conformational changes induced when ACh binds to remaining sites. 3. Equations based on this proposal are developed and are shown to provide an accurate description of the entire e.p.c. time couse as modified by eith QX-222 or QX-314. 4. Other models are also discussed; models in which local anaesthetic molecules bind only after receptor activation appear to be ruled out.", "contents": "A quantitative description of end-plate currents in the presence of two lidocaine derivatives. 1. Possible ways in which local anaesthetics may act to produce their characteristic alterations of end-plate current (e.p.c.s) are considered. 2. Following Magleby & Stevens (1972b), it is proposed that when acetylcholine (ACh) binds to its end-plate receptor it induces a conformational change which causes an increased ionic permeability and, thus, the measured e.p.c.; the reverse conformational change which returns the permeability to its resting state is postulated to be the rate-limiting event for e.p.c. decay whether or not local anaesthetics are present. Moreover, it is proposed that the ACh receptor had multiple binding sites and the local anaesthetics can bind to one or more of these sites thereby altering the conformational changes induced when ACh binds to remaining sites. 3. Equations based on this proposal are developed and are shown to provide an accurate description of the entire e.p.c. time couse as modified by eith QX-222 or QX-314. 4. Other models are also discussed; models in which local anaesthetic molecules bind only after receptor activation appear to be ruled out.", "PMID": 957158}
{"id": "pubmed23n0032_2600", "title": "Analysis of spontaneous activity of auditory neurones in the spiral ganglion of the guinea-pig cochlea.", "content": "1. Spontaneous activity of single afferent neurones in the cochlea of the guinea-pig was studied by measurement of mean rates and computation of interspike interval histograms. 2. The shapes of interval histograms agreed with those found in cat cochlear nerve fibres. Modes of the histograms were less than 7 msec for all cells with mean rates ranging from 20 to 150 spikes/sec. 3. The distribution of mean rates showed higher maximum rates than those found in cat. Cells from animals with abnormally high thresholds showed lower mean rates than those from sensitive animals. 4. No correlation was found between mean spontaneous rate and sensitivity to acoustic stimulation. No discrete populations of cells could be discerned on any of the criteria used. 5. The simultaneous effects of hypoxia on cell thresholds, mean spontaneous rate and scala media potentials are reported. These and other results are discussed in relation to possible sites of generation of spontaneous activity in primary auditory afferents.", "contents": "Analysis of spontaneous activity of auditory neurones in the spiral ganglion of the guinea-pig cochlea. 1. Spontaneous activity of single afferent neurones in the cochlea of the guinea-pig was studied by measurement of mean rates and computation of interspike interval histograms. 2. The shapes of interval histograms agreed with those found in cat cochlear nerve fibres. Modes of the histograms were less than 7 msec for all cells with mean rates ranging from 20 to 150 spikes/sec. 3. The distribution of mean rates showed higher maximum rates than those found in cat. Cells from animals with abnormally high thresholds showed lower mean rates than those from sensitive animals. 4. No correlation was found between mean spontaneous rate and sensitivity to acoustic stimulation. No discrete populations of cells could be discerned on any of the criteria used. 5. The simultaneous effects of hypoxia on cell thresholds, mean spontaneous rate and scala media potentials are reported. These and other results are discussed in relation to possible sites of generation of spontaneous activity in primary auditory afferents.", "PMID": 957159}
{"id": "pubmed23n0032_2601", "title": "Vasodepressor effects obtained by drugs acting on the ventral surface of the brain stem.", "content": "1. In cats anaesthetized with I.P. pentobarbitone sodium and atropinized with intravenous atropine methyl nitrate, the effects on arterial blood pressure were examined of nicotine, physostigmine, carbachol, glycine and pentobarbitone sodium applied to the exposed ventral surface of the brain stem by means of paired Perspex rings placed across the medulla. The drugs were placed inside each ring in a volume of 10mul. 2. Nicotine (0-5-6mg/ml.) produced a fall in blood pressure when the upper limit of the areas covered by the rings was about 5-6 mm caudal, but not when it was just caudal to the trapezoid bodies. The depressor effect was obtained both on bilateral and unilateral application. After application the nicotine sensitive area became for several minutes insensitive to its renewed application. 3. The depressor effect of nicotine was sensitive to hexamethonium (50 mg/ml.) but resistant to atropine (50 mg/ml.) similarly applied. 4. When the nicotine sensitive areas had become insensitive to nicotine, bilateral carotid occulusion produced its normal sustained pressor response. 5. By applying the nicotine through a single Perspex ring which could be moved stepwise along and across the medulla, the nicotine sensitive area was localized and the highest sensitivity was found in a region around and a little caudal to the rootlets of the XIIth cranial nerve. 6. Physostigmine (25 and 50 mg/ml.) and carbachol (6 mg/ml.) produced a fall in blood pressure when the uppermost limits of the areas covered by the paired rings were 5-6 mm caudal to and also when they were just caudal to the trapezoid bodies. From both regions the effects were obtained on bilateral and unilateral application. Their depressor effects were sensitive to atropine but resistant to hexamethonium similarly applied. 7. Glycine (200 mg/ml.) and pentobarbitone sodium (100 mg/ml.) produced scarcely any blood pressure effects when applied bilaterally to the nicotine sensitive areas but produced strong depressor effects on more rostral application, i.e. when the uppermost limit of the areas covered by the rings were just caudal to the trapezoid bodies. 8. It is concluded that the ventral surface of the medulla contains at least two bilateral areas from which vasodepressor effects are obtained on topical application of drugs: a more caudally situated one which is sensitive to nicotine, but insensitive to glycine and pentobarbitone sodium; a more rostrally situated one which is insensitive to nicotine but sensitive to glycine and pentobarbitone sodium; and both areas are sensitive to carbachol and physostigmine.", "contents": "Vasodepressor effects obtained by drugs acting on the ventral surface of the brain stem. 1. In cats anaesthetized with I.P. pentobarbitone sodium and atropinized with intravenous atropine methyl nitrate, the effects on arterial blood pressure were examined of nicotine, physostigmine, carbachol, glycine and pentobarbitone sodium applied to the exposed ventral surface of the brain stem by means of paired Perspex rings placed across the medulla. The drugs were placed inside each ring in a volume of 10mul. 2. Nicotine (0-5-6mg/ml.) produced a fall in blood pressure when the upper limit of the areas covered by the rings was about 5-6 mm caudal, but not when it was just caudal to the trapezoid bodies. The depressor effect was obtained both on bilateral and unilateral application. After application the nicotine sensitive area became for several minutes insensitive to its renewed application. 3. The depressor effect of nicotine was sensitive to hexamethonium (50 mg/ml.) but resistant to atropine (50 mg/ml.) similarly applied. 4. When the nicotine sensitive areas had become insensitive to nicotine, bilateral carotid occulusion produced its normal sustained pressor response. 5. By applying the nicotine through a single Perspex ring which could be moved stepwise along and across the medulla, the nicotine sensitive area was localized and the highest sensitivity was found in a region around and a little caudal to the rootlets of the XIIth cranial nerve. 6. Physostigmine (25 and 50 mg/ml.) and carbachol (6 mg/ml.) produced a fall in blood pressure when the uppermost limits of the areas covered by the paired rings were 5-6 mm caudal to and also when they were just caudal to the trapezoid bodies. From both regions the effects were obtained on bilateral and unilateral application. Their depressor effects were sensitive to atropine but resistant to hexamethonium similarly applied. 7. Glycine (200 mg/ml.) and pentobarbitone sodium (100 mg/ml.) produced scarcely any blood pressure effects when applied bilaterally to the nicotine sensitive areas but produced strong depressor effects on more rostral application, i.e. when the uppermost limit of the areas covered by the rings were just caudal to the trapezoid bodies. 8. It is concluded that the ventral surface of the medulla contains at least two bilateral areas from which vasodepressor effects are obtained on topical application of drugs: a more caudally situated one which is sensitive to nicotine, but insensitive to glycine and pentobarbitone sodium; a more rostrally situated one which is insensitive to nicotine but sensitive to glycine and pentobarbitone sodium; and both areas are sensitive to carbachol and physostigmine.", "PMID": 957160}
{"id": "pubmed23n0032_2602", "title": "The effect of temperature on potassium chloride contracture in cat myocardium.", "content": "1. Contracture was induced in cat myocardium by exposure to 140 mM-KC1 In isotonic Tyrode solution. Force of contracture expressed as mg/mm2 (muscle cross-sectional area) falls with increasing cross-sectional area. 2. The effect of temperature on isometric force developed during contracture was evaluated both in normal (untreated) atrial and ventricular muscle and following treatment with sympatholytic drugs. 3. The force of contracture was not significantly affected by sympatholytic drugs at 36 degrees C. 4. In normal atrial and ventricular muscle, force of contracture decreased when the muscle was cooled from 36 to either 29 or 20 degrees C. 5. In atrial muscle, the effect of temperature was not changed by sympatholytic drugs. In contrast, exposure to sympatholytic drugs increased contracture force developed by ventricular muscle at 20 degrees C. Also, contracture force was significantly greater at 20 than at 36 degrees C in ventricular muscle from reserpine-pretreated cats. 6. It is suggested that ventricular muscle becomes more sensitive to the relaxing effects of endogenous catecholamines at temperature is lowered. 7. The differences shown between atrial and ventricular muscle with respect to the effect of temperature and sympatholytic drugs on contracture force may result from the differing amounts of sarcoplasmic reticulum found in these types of cardiac muscle and also from different mechanisms of \"excitation-contracture\" coupling in atrial and ventricular muscle.", "contents": "The effect of temperature on potassium chloride contracture in cat myocardium. 1. Contracture was induced in cat myocardium by exposure to 140 mM-KC1 In isotonic Tyrode solution. Force of contracture expressed as mg/mm2 (muscle cross-sectional area) falls with increasing cross-sectional area. 2. The effect of temperature on isometric force developed during contracture was evaluated both in normal (untreated) atrial and ventricular muscle and following treatment with sympatholytic drugs. 3. The force of contracture was not significantly affected by sympatholytic drugs at 36 degrees C. 4. In normal atrial and ventricular muscle, force of contracture decreased when the muscle was cooled from 36 to either 29 or 20 degrees C. 5. In atrial muscle, the effect of temperature was not changed by sympatholytic drugs. In contrast, exposure to sympatholytic drugs increased contracture force developed by ventricular muscle at 20 degrees C. Also, contracture force was significantly greater at 20 than at 36 degrees C in ventricular muscle from reserpine-pretreated cats. 6. It is suggested that ventricular muscle becomes more sensitive to the relaxing effects of endogenous catecholamines at temperature is lowered. 7. The differences shown between atrial and ventricular muscle with respect to the effect of temperature and sympatholytic drugs on contracture force may result from the differing amounts of sarcoplasmic reticulum found in these types of cardiac muscle and also from different mechanisms of \"excitation-contracture\" coupling in atrial and ventricular muscle.", "PMID": 957161}
{"id": "pubmed23n0032_2603", "title": "Active sodium uptake by the skin of foetal sheep and pigs.", "content": "1. Simultaneous measurements of unidirectional sodium fluxes across foetal skin incubated in vitro with identical solutions ([Na] = 150 mM) bathing either side showed a flux ratio (influx/efflux) of 1-40+/-0-08 in twenty-seven sheep skins, which was significantly different from unity (P less than 0-001). The gestational ages ranged from 47 to 98 days (term = 147 days). Similar experiments on eight foetal pig skins at 58 days gestation (term = 114-118 days) gave a mean flux ratio of 1-10 +/- 0-03 (P less than 0-02). 2. Unidirectional sodium fluxes measured with dilute Ringer solution on the outside (mucosal) surface ([Na]0 = 100mM) gave influx to efflux ratios of 0-86 +/- 0-09 in seventeen sheep (P less than 0-05) and 1-07 +/- 0-26 in five foetal pigs; the value predicted for passive movement was 0-67. 3. Incubation with inhibitors, ouabain (10-4 M) or dinitrophenol (DNP) (10-4 M) gave a flux ratio for sodium which was not significantly different from unity in the absence of a gradient, or from 0-67 when the concentration gradient was applied. 4. Sequential measurement of unidirectional diffusional fluxes of tritiated water across foetal skin gave flux ratios of 0-98 +/- 0-02 in six sheep skins and 1-06 +/- 0-11 for four pig skins in control conditions. When the outside solution was diluted to give an osmotic gradient of 100 m-osmole. kg-1 across the skin a flux ratio of 0-95 +/- 0-07 was obtained for seven sheep and was not measured in pig skin. Hormones and inhibitors had no effect on the diffusional flux ratio for water in the presence or absence of an osmotic gradient. 5. Lysine vasopressin (ADH) (200 mu./ml.) increased influx and efflux of water in the presence and, to a lesser extent in the absence of an osmotic gradient in sheep skin. In pig skin prolactin (1 u./ml.) increased both influx and efflux, but ADH had no effect on diffusional water fluxes. 6. ADH increased sodium influx in sheep skin slightly but vasotocin (5-5 mu./ml.) was more potent, particularly in the presence of an opposing diffusion gradient. Vasotocin (55 mu./ml.) reduced sodium influx in pig skin ADH had no effect on influx or efflux and prolactin reduced sodium influx and efflux. Ouabain and DNP generally reduced permeability to both sodium and water in sheep skin but had no effect in pig skin.", "contents": "Active sodium uptake by the skin of foetal sheep and pigs. 1. Simultaneous measurements of unidirectional sodium fluxes across foetal skin incubated in vitro with identical solutions ([Na] = 150 mM) bathing either side showed a flux ratio (influx/efflux) of 1-40+/-0-08 in twenty-seven sheep skins, which was significantly different from unity (P less than 0-001). The gestational ages ranged from 47 to 98 days (term = 147 days). Similar experiments on eight foetal pig skins at 58 days gestation (term = 114-118 days) gave a mean flux ratio of 1-10 +/- 0-03 (P less than 0-02). 2. Unidirectional sodium fluxes measured with dilute Ringer solution on the outside (mucosal) surface ([Na]0 = 100mM) gave influx to efflux ratios of 0-86 +/- 0-09 in seventeen sheep (P less than 0-05) and 1-07 +/- 0-26 in five foetal pigs; the value predicted for passive movement was 0-67. 3. Incubation with inhibitors, ouabain (10-4 M) or dinitrophenol (DNP) (10-4 M) gave a flux ratio for sodium which was not significantly different from unity in the absence of a gradient, or from 0-67 when the concentration gradient was applied. 4. Sequential measurement of unidirectional diffusional fluxes of tritiated water across foetal skin gave flux ratios of 0-98 +/- 0-02 in six sheep skins and 1-06 +/- 0-11 for four pig skins in control conditions. When the outside solution was diluted to give an osmotic gradient of 100 m-osmole. kg-1 across the skin a flux ratio of 0-95 +/- 0-07 was obtained for seven sheep and was not measured in pig skin. Hormones and inhibitors had no effect on the diffusional flux ratio for water in the presence or absence of an osmotic gradient. 5. Lysine vasopressin (ADH) (200 mu./ml.) increased influx and efflux of water in the presence and, to a lesser extent in the absence of an osmotic gradient in sheep skin. In pig skin prolactin (1 u./ml.) increased both influx and efflux, but ADH had no effect on diffusional water fluxes. 6. ADH increased sodium influx in sheep skin slightly but vasotocin (5-5 mu./ml.) was more potent, particularly in the presence of an opposing diffusion gradient. Vasotocin (55 mu./ml.) reduced sodium influx in pig skin ADH had no effect on influx or efflux and prolactin reduced sodium influx and efflux. Ouabain and DNP generally reduced permeability to both sodium and water in sheep skin but had no effect in pig skin.", "PMID": 957162}
{"id": "pubmed23n0032_2604", "title": "The effect of hormones and of an osmotic gradient on the structure and properties of mammalian foetal urinary bladder in vitro.", "content": "1. Water and isotope fluxes were measured by incubating urinary bladders of foetal pigs and sheep in vitro in the presence and absence of a concentration and osmotic gradient. The structure of the urinary bladder of foetal pigs under various conditions was studied by electron microscopy. Its ultrastructure was found to be closely similar to that of foetal sheep. 2. Antidiuretic hormone (ADH) (0-2 U. ml-1) enhanced the enlargement of intercellular spaces caused by dilute mucosal medium in pig bladders; prolactin (1 u. ml-1) prevented osmotic dilatation of the intercellular spaces. 3. The hydraulic conductivity, Lp, was estimated to be 0-5 X 10 (-7) cm.s-1atm-1 in sheep and pigs at about 100 days gestation; the ratio of osomotic to diffusional permeability, (LpRT/VW)/PD, in the presence and absence of ADH, was 2-1 and 1-6 respectively. These are similar to the values found in fish gills. 4. Prolactin reduced bulk flow of water to zero in seven out of eight bladders investigated. Incubation with ADH or vasotocin (55 mu. ml-1) in the presence of prolactin restored water flux to 22% and 45% of control values respectively. 5. There was no significant net flux of sodium from mucosa to serosa in pig bladder except in the presence of prolactin. No net flux of sodium occurred from mucosal to serosal side of pig or sheep bladders in the presence of an adverse electrochemical gradient, although in sheep the permeability ratio was significantly greater than one. 6. The diffusional flux ratio for water remained unity under all conditions; vasotocin increased unidirectional fluxes and prolactin reduced them. The flux ratios were unaffected by the direction of bulk fluid flow, probably because diffusion was rapid compared to flow: the ratio of diffusional flux to volume flow was between 11 and 18.", "contents": "The effect of hormones and of an osmotic gradient on the structure and properties of mammalian foetal urinary bladder in vitro. 1. Water and isotope fluxes were measured by incubating urinary bladders of foetal pigs and sheep in vitro in the presence and absence of a concentration and osmotic gradient. The structure of the urinary bladder of foetal pigs under various conditions was studied by electron microscopy. Its ultrastructure was found to be closely similar to that of foetal sheep. 2. Antidiuretic hormone (ADH) (0-2 U. ml-1) enhanced the enlargement of intercellular spaces caused by dilute mucosal medium in pig bladders; prolactin (1 u. ml-1) prevented osmotic dilatation of the intercellular spaces. 3. The hydraulic conductivity, Lp, was estimated to be 0-5 X 10 (-7) cm.s-1atm-1 in sheep and pigs at about 100 days gestation; the ratio of osomotic to diffusional permeability, (LpRT/VW)/PD, in the presence and absence of ADH, was 2-1 and 1-6 respectively. These are similar to the values found in fish gills. 4. Prolactin reduced bulk flow of water to zero in seven out of eight bladders investigated. Incubation with ADH or vasotocin (55 mu. ml-1) in the presence of prolactin restored water flux to 22% and 45% of control values respectively. 5. There was no significant net flux of sodium from mucosa to serosa in pig bladder except in the presence of prolactin. No net flux of sodium occurred from mucosal to serosal side of pig or sheep bladders in the presence of an adverse electrochemical gradient, although in sheep the permeability ratio was significantly greater than one. 6. The diffusional flux ratio for water remained unity under all conditions; vasotocin increased unidirectional fluxes and prolactin reduced them. The flux ratios were unaffected by the direction of bulk fluid flow, probably because diffusion was rapid compared to flow: the ratio of diffusional flux to volume flow was between 11 and 18.", "PMID": 957164}
{"id": "pubmed23n0032_2605", "title": "Location of stretch receptors in the trachea and bronchi of the dog.", "content": "1. Using single fibre vagal afferent recording techniques, we have investigated the longitudinal and circumferential location of slowly adapting stretch receptors in a functionally isolated, in situ segment of the trachea in dogs. 2. We have also studied the depth of these receptors within the airway wall and their response to reflex and drug induced contraction of the airway smooth muscle. 3. Thirty-four per cent of the receptors studied were in the extrathoracic trachea; calculations indicate that about 17-1% of all airway stretch receptors lie outside the thorax. 4. All the receptors were located in the membranous posterior wall of the trachea, and those tested responded more to transverse than to longitudinal stretching of the wall. 5. Receptors in the extrathoracic trachea continued to function after the regional mucosa had been widely resected; subsequent removal of histolocially proven smooth muscle tissue caused the abrupt cessation of receptor discharge. 6. Contraction of airway smooth muscle brought about an increase in receptor discharge frequency. 7. Similar results were found for a smaller number of stretch receptors located in the mainstem and lobar bronchi. 8. These findings procided evidence that stretch receptors are anatomically located within the smooth muscle layer of the airways and are functionally aranged in series with the muscle fibres.", "contents": "Location of stretch receptors in the trachea and bronchi of the dog. 1. Using single fibre vagal afferent recording techniques, we have investigated the longitudinal and circumferential location of slowly adapting stretch receptors in a functionally isolated, in situ segment of the trachea in dogs. 2. We have also studied the depth of these receptors within the airway wall and their response to reflex and drug induced contraction of the airway smooth muscle. 3. Thirty-four per cent of the receptors studied were in the extrathoracic trachea; calculations indicate that about 17-1% of all airway stretch receptors lie outside the thorax. 4. All the receptors were located in the membranous posterior wall of the trachea, and those tested responded more to transverse than to longitudinal stretching of the wall. 5. Receptors in the extrathoracic trachea continued to function after the regional mucosa had been widely resected; subsequent removal of histolocially proven smooth muscle tissue caused the abrupt cessation of receptor discharge. 6. Contraction of airway smooth muscle brought about an increase in receptor discharge frequency. 7. Similar results were found for a smaller number of stretch receptors located in the mainstem and lobar bronchi. 8. These findings procided evidence that stretch receptors are anatomically located within the smooth muscle layer of the airways and are functionally aranged in series with the muscle fibres.", "PMID": 957165}
{"id": "pubmed23n0032_2606", "title": "Transduction properties of tracheal stretch receptors.", "content": "1. Using single fibre vagal afferent recording, we have studied the behavior of slowly adapting stretch receptors located in an isolated, in situ, segment of the trachea in dogs. Responses to positive and negative steady and oscillating transmural pressures were investigated. 2. Seventy-eight per cent of the receptors studied were tonically active at resting tracheal volume. Ninety per cent showed a more pronounced response to positive than to negative transmural pressures. 3. During pressure oscillations the majority of the receptors had a higher discharge frequency at any given pressure during the ascending phase of the pressure wave than at the same pressure under static conditions. During most of the ensuing descent of pressure toward zero the discharge frequency led transmural pressure. 4. With increasing frequency of oscillation the differences from the static responses increased (dP/dt sensitivity), especially during the ascending limb of the pressure oscillation (rectifying behavior). 5. In a small number of receptors, discharge frequency lagged behind transmural pressure or was in phase with it (\"no loop\" pattern). 6. In three cases the same receptor exhibited dP/dt sensitivity during positive pressure oscillations, whereas discharge frequency lagged behind pressure during negative pressure oscillations. This indicates that the lack of dP/dt sensitivity exhibited under negative pressure conditions does not represent an intrinsic property of these receptors, but reflects some aspect of their mechanical arrangement within the airway wall. 7. THESE PATTERNS OF RESPONSE ARE DISCUSSed in terms of intrinsic and extrinsic characteristics of the receptors. 8. The physiological implications  of stretch receptor behaviour are also considered.", "contents": "Transduction properties of tracheal stretch receptors. 1. Using single fibre vagal afferent recording, we have studied the behavior of slowly adapting stretch receptors located in an isolated, in situ, segment of the trachea in dogs. Responses to positive and negative steady and oscillating transmural pressures were investigated. 2. Seventy-eight per cent of the receptors studied were tonically active at resting tracheal volume. Ninety per cent showed a more pronounced response to positive than to negative transmural pressures. 3. During pressure oscillations the majority of the receptors had a higher discharge frequency at any given pressure during the ascending phase of the pressure wave than at the same pressure under static conditions. During most of the ensuing descent of pressure toward zero the discharge frequency led transmural pressure. 4. With increasing frequency of oscillation the differences from the static responses increased (dP/dt sensitivity), especially during the ascending limb of the pressure oscillation (rectifying behavior). 5. In a small number of receptors, discharge frequency lagged behind transmural pressure or was in phase with it (\"no loop\" pattern). 6. In three cases the same receptor exhibited dP/dt sensitivity during positive pressure oscillations, whereas discharge frequency lagged behind pressure during negative pressure oscillations. This indicates that the lack of dP/dt sensitivity exhibited under negative pressure conditions does not represent an intrinsic property of these receptors, but reflects some aspect of their mechanical arrangement within the airway wall. 7. THESE PATTERNS OF RESPONSE ARE DISCUSSed in terms of intrinsic and extrinsic characteristics of the receptors. 8. The physiological implications  of stretch receptor behaviour are also considered.", "PMID": 957167}
{"id": "pubmed23n0032_2607", "title": "Release of peptide hydrolases during incubation of intact intestinal segments in vitro.", "content": "1. Rat intestinal segments have been incubated in isotonic saline in vitro and release of peptide hydrolase enzymes into the incubation media examined over a 90 min study period. 2. Chemical assay data, as well as analysis of electrophoretic mobilities of release enzymes on starch gel, indicate that peptide hydrolase enzymes in the incubation media originate predominantly from the cytoplasm of the mucosal cells. 3. Peptide hydrolases were released rapidly from intact intestinal segments. Release occurred from the start of the in vitro incubations and was not affected by temperature and shaking. 4. It is concluded that compared to the in vivo situation, cytoplasmic peptide hydrolases are released from intestinal tissue very rapidly in vitro. Caution is therefore required when comparing results of in vivo and in vitro peptide absorption experiments.", "contents": "Release of peptide hydrolases during incubation of intact intestinal segments in vitro. 1. Rat intestinal segments have been incubated in isotonic saline in vitro and release of peptide hydrolase enzymes into the incubation media examined over a 90 min study period. 2. Chemical assay data, as well as analysis of electrophoretic mobilities of release enzymes on starch gel, indicate that peptide hydrolase enzymes in the incubation media originate predominantly from the cytoplasm of the mucosal cells. 3. Peptide hydrolases were released rapidly from intact intestinal segments. Release occurred from the start of the in vitro incubations and was not affected by temperature and shaking. 4. It is concluded that compared to the in vivo situation, cytoplasmic peptide hydrolases are released from intestinal tissue very rapidly in vitro. Caution is therefore required when comparing results of in vivo and in vitro peptide absorption experiments.", "PMID": 957171}
{"id": "pubmed23n0032_2608", "title": "Sites of autoregulatory escape of blood flow in the mesenteric vascular bed.", "content": "1. Stimulation of the sympathetic nerves to the intestinal vascular bed results in an initial decrease in blood flow followed by a recovery towards the control level. This recovery was termed autoregulatory escape by Folkow and his co-workers and they suggested it was associated with a redistribution of blood flow within the intestinal wall. This theory has been examined in cats anaesthetized with pentobarbitone sodium. 2. The sympathetic nerves to the intestinal vascular bed were stimulated for 4 min periods at a submaximal frequency (4 Hz). The blood flows to individual parts of the superior mesenteric arterial bed (whole intestine, mucosal and submucosal layer, muscle layer of intestine, mesentery and lymph nodes, appendix and colon) were measured using radioactive microspheres before, at the peak of the vasoconstriction (30 sec), after autoregulatory escape had occurred (3-5 min) and during the hyperaemia after cessation of nerve stimulation. 3. All parts of the mesenteric vascular bed showed a significant initial vasoconstriction followed by a recovery in the flow to a level not significantly different from the pre-stimulation control flow. All parts showed a significant hyperaemia after cessation of stimulation. The distribution of the superior mesenteric flow at the peak of the vasoconstriction, after autoregulatory escape had occurred and during the hyperaemia after cessation of nerve stimulation was not significantly different from that during the control period. 4. It is concluded that all parts of the mesenteric vascular bed show autoregulatory escape and that this phenomenon is not associated with a redistribution of blood flow within the intestinal wall. Autoregulatory escape must involve relaxation of the same vessels which were originally constricted and various theories on the mechanism of the escape are discussed.", "contents": "Sites of autoregulatory escape of blood flow in the mesenteric vascular bed. 1. Stimulation of the sympathetic nerves to the intestinal vascular bed results in an initial decrease in blood flow followed by a recovery towards the control level. This recovery was termed autoregulatory escape by Folkow and his co-workers and they suggested it was associated with a redistribution of blood flow within the intestinal wall. This theory has been examined in cats anaesthetized with pentobarbitone sodium. 2. The sympathetic nerves to the intestinal vascular bed were stimulated for 4 min periods at a submaximal frequency (4 Hz). The blood flows to individual parts of the superior mesenteric arterial bed (whole intestine, mucosal and submucosal layer, muscle layer of intestine, mesentery and lymph nodes, appendix and colon) were measured using radioactive microspheres before, at the peak of the vasoconstriction (30 sec), after autoregulatory escape had occurred (3-5 min) and during the hyperaemia after cessation of nerve stimulation. 3. All parts of the mesenteric vascular bed showed a significant initial vasoconstriction followed by a recovery in the flow to a level not significantly different from the pre-stimulation control flow. All parts showed a significant hyperaemia after cessation of stimulation. The distribution of the superior mesenteric flow at the peak of the vasoconstriction, after autoregulatory escape had occurred and during the hyperaemia after cessation of nerve stimulation was not significantly different from that during the control period. 4. It is concluded that all parts of the mesenteric vascular bed show autoregulatory escape and that this phenomenon is not associated with a redistribution of blood flow within the intestinal wall. Autoregulatory escape must involve relaxation of the same vessels which were originally constricted and various theories on the mechanism of the escape are discussed.", "PMID": 957204}
{"id": "pubmed23n0032_2609", "title": "Functional and structural changes in mammalian sympathetic neurones following colchicine application to post-ganglionic nerves.", "content": "1. The effects of post-ganglionic colchicine application on neurones of the guinea-pig superior cervical ganglion were studied with intracellular recording and electron microscopy. 2. Local colchicine application for 30 min to one of the major post-ganglionic nerves caused several electrophysiological changes after 4-7 days in many neurones whose axons run in this nerve. These changes include: (a) a reduction in the amplitude of synaptic potentials elicited by supramaximal preganglionic stimulation; (b) a decrease in the number of preganglionic fibres innervating individual neurones; (c) the development of regenerative responses in dendrites; and (d) the failure of antidromic action potentials to fully invade the neuronal soma. These functional changes occurred in the absence of impaired impulse conduction or axon degeneration, and were not observed in nearby neurones whose axons ran in an untreated post-ganglionic nerve. The effects of colchicine are similar to the changes produced by axotomy. 3. Counts of synapses in thin sections from the region of the ganglion where the affected neurones were located showed a reduction, compared to the number of synapses in other regions of the colchicine treated ganglia, or normal control ganglia. This finding indicates that synaptic depression after colchicine treatment, like that after axotomy, is due primarily to a loss of synaptic contacts from the dendrites of affected nerve cells. Unusual profiles containing numerous vesicular and tubular organelles frequently seen after interruption of the axons were also observed in thin sections after colchicine treatment. 4. The similarity of the electrophysiological and ultrastructural effects of colchicine treatment and axon interruption offers further support for the view that synaptic contacts on sympathetic neurones are normally regulated by an interaction of the neuronal soma with its axonal extension to the periphery.", "contents": "Functional and structural changes in mammalian sympathetic neurones following colchicine application to post-ganglionic nerves. 1. The effects of post-ganglionic colchicine application on neurones of the guinea-pig superior cervical ganglion were studied with intracellular recording and electron microscopy. 2. Local colchicine application for 30 min to one of the major post-ganglionic nerves caused several electrophysiological changes after 4-7 days in many neurones whose axons run in this nerve. These changes include: (a) a reduction in the amplitude of synaptic potentials elicited by supramaximal preganglionic stimulation; (b) a decrease in the number of preganglionic fibres innervating individual neurones; (c) the development of regenerative responses in dendrites; and (d) the failure of antidromic action potentials to fully invade the neuronal soma. These functional changes occurred in the absence of impaired impulse conduction or axon degeneration, and were not observed in nearby neurones whose axons ran in an untreated post-ganglionic nerve. The effects of colchicine are similar to the changes produced by axotomy. 3. Counts of synapses in thin sections from the region of the ganglion where the affected neurones were located showed a reduction, compared to the number of synapses in other regions of the colchicine treated ganglia, or normal control ganglia. This finding indicates that synaptic depression after colchicine treatment, like that after axotomy, is due primarily to a loss of synaptic contacts from the dendrites of affected nerve cells. Unusual profiles containing numerous vesicular and tubular organelles frequently seen after interruption of the axons were also observed in thin sections after colchicine treatment. 4. The similarity of the electrophysiological and ultrastructural effects of colchicine treatment and axon interruption offers further support for the view that synaptic contacts on sympathetic neurones are normally regulated by an interaction of the neuronal soma with its axonal extension to the periphery.", "PMID": 957208}
{"id": "pubmed23n0032_2610", "title": "Activation of two types of fibres in rat extraocular muscles.", "content": "1. The contractile responses of the inferior rectus, one of the extraocular muscles of the rat, to a depolarization induced by an elevation of the potassium concentration in the external medium ([K]O) have been studied 'in vitro'. 2. The elevation of [K]O to 20 and 30 mM-K produced contractures that consisted of a sustained or tonic tension. When [K]O was increased to 50 mM or more a well-defined transient or phasic tension appeared before the tonic response. The increment of [K]O above 50 mM enhanced the phasic component and depressed the tonic tension. The maximal tonic tension, usually evoked by 50 mM-K, is about 50% of the tetanic tension, shows a gradual decline with time and lasts for hours. Control experiments performed in diaphragm showed that this muscle only responds with phasic tensions. 3. The difference in the repriming of the phasic and tonic responses when tensions were induced with salines containing low or normal [Cl] suggests that the muscle fibres responsible for the tonic tension are poorly permeable to Cl-. 4. The amplitude of the tonic tension was reduced by Ca deprivation and by an elevation of [Ca] in the saline to 10 mM. 5. It is concluded that in rat extraocular muscles, an increase in [K]O activates two types of muscle fibres: singly and multiply innervated. These appear to be functionally equivalent to the twitch and slow fibres of amphibian and avian muscle and would give rise to the phasic and tonic components of the contracture, respectively.", "contents": "Activation of two types of fibres in rat extraocular muscles. 1. The contractile responses of the inferior rectus, one of the extraocular muscles of the rat, to a depolarization induced by an elevation of the potassium concentration in the external medium ([K]O) have been studied 'in vitro'. 2. The elevation of [K]O to 20 and 30 mM-K produced contractures that consisted of a sustained or tonic tension. When [K]O was increased to 50 mM or more a well-defined transient or phasic tension appeared before the tonic response. The increment of [K]O above 50 mM enhanced the phasic component and depressed the tonic tension. The maximal tonic tension, usually evoked by 50 mM-K, is about 50% of the tetanic tension, shows a gradual decline with time and lasts for hours. Control experiments performed in diaphragm showed that this muscle only responds with phasic tensions. 3. The difference in the repriming of the phasic and tonic responses when tensions were induced with salines containing low or normal [Cl] suggests that the muscle fibres responsible for the tonic tension are poorly permeable to Cl-. 4. The amplitude of the tonic tension was reduced by Ca deprivation and by an elevation of [Ca] in the saline to 10 mM. 5. It is concluded that in rat extraocular muscles, an increase in [K]O activates two types of muscle fibres: singly and multiply innervated. These appear to be functionally equivalent to the twitch and slow fibres of amphibian and avian muscle and would give rise to the phasic and tonic components of the contracture, respectively.", "PMID": 957210}
{"id": "pubmed23n0032_2611", "title": "Regulation of cerebrospinal fluid potassium by the cat choroid plexus.", "content": "1. The regulation of cerebrospinal fluid (c.s.f.) potassium concentration was studied using the cat choroid plexus isolated in a chamber in situ. 2. Hyperkalaemia (plasma potassium concentration greater than 6 m-equiv/l.) caused relatively small increases in c.s.f. potassium concentration. 3. Alterations in c.s.f. potassium concentration (c.s.f. K = 0-15 or 6-56 m-equiv/l.) were countered by changes in potassium concentration of the produced fluid or in the rate of potassium transport which returned c.s.f. potassium towards normal. 4. The data indicate that potassium concentration in c.s.f. secreted by the choroid plexus is actively regulated by the plexus whether the primary alteration in potassium occurs in plasma or c.s.f.", "contents": "Regulation of cerebrospinal fluid potassium by the cat choroid plexus. 1. The regulation of cerebrospinal fluid (c.s.f.) potassium concentration was studied using the cat choroid plexus isolated in a chamber in situ. 2. Hyperkalaemia (plasma potassium concentration greater than 6 m-equiv/l.) caused relatively small increases in c.s.f. potassium concentration. 3. Alterations in c.s.f. potassium concentration (c.s.f. K = 0-15 or 6-56 m-equiv/l.) were countered by changes in potassium concentration of the produced fluid or in the rate of potassium transport which returned c.s.f. potassium towards normal. 4. The data indicate that potassium concentration in c.s.f. secreted by the choroid plexus is actively regulated by the plexus whether the primary alteration in potassium occurs in plasma or c.s.f.", "PMID": 957213}
{"id": "pubmed23n0032_2612", "title": "Reflex activation of sympathetic pathways to vesical smooth muscle and parasympathetic ganglia by electrical stimulation of vesical afferents.", "content": "1. Activation of the sympathetic input to the urinary bladder by electrical stimulation of afferent fibres in the pelvic nerve evoked three responses: (1) an initial transient rise in intravesical pressure, (2) an inhibition of neurally evoked bladder contractions and (3) an inhibition of transmission in vesical parasympathetic ganglia. Similar responses were elicited by stimulation of the hypogastric nerve. 2. The reflex responses were observed in acute spinal preparations (T10-T12) and in cats with intact spinal cords, but were abolished by bilateral transection of the hypogastric nerves. 3. The inhibition of bladder contractions was antagonized by the administration of propranolol (200-400 mug, I.A.), a beta-adrenergic blocking agent. The inhibition of ganglionic transmission was antagonized by dihydroergotamine (30-75 mug, I.A.), an alpha-adrenergic blocking agent. The initial rise in intravesical pressure was not antagonized by either agent. 4. Electrical stimulation of other afferents (carotid sinus nerve and sciatic nerve) did not consistently elicit responses in the urinary bladder. However, stimulation of these afferents as well as pelvic nerve afferents evoked reflex firing in nerve filaments on the surface of the urinary bladder. The firing was abolished by transection of the ipsilateral hypogastric nerve. 5. It is concluded that stimulation of vesical afferents activates a spinal sympathetic reflex which results in closing of the internal urethral sphincter and a depression of bladder activity. The latter occurs by a direct depression of detrusor smooth muscle as well as a block of the neural input to the bladder. This vesico-sympathetic reflex represents a negative feed-back mechanism which may have an important role in the maintenance of urinary continence.", "contents": "Reflex activation of sympathetic pathways to vesical smooth muscle and parasympathetic ganglia by electrical stimulation of vesical afferents. 1. Activation of the sympathetic input to the urinary bladder by electrical stimulation of afferent fibres in the pelvic nerve evoked three responses: (1) an initial transient rise in intravesical pressure, (2) an inhibition of neurally evoked bladder contractions and (3) an inhibition of transmission in vesical parasympathetic ganglia. Similar responses were elicited by stimulation of the hypogastric nerve. 2. The reflex responses were observed in acute spinal preparations (T10-T12) and in cats with intact spinal cords, but were abolished by bilateral transection of the hypogastric nerves. 3. The inhibition of bladder contractions was antagonized by the administration of propranolol (200-400 mug, I.A.), a beta-adrenergic blocking agent. The inhibition of ganglionic transmission was antagonized by dihydroergotamine (30-75 mug, I.A.), an alpha-adrenergic blocking agent. The initial rise in intravesical pressure was not antagonized by either agent. 4. Electrical stimulation of other afferents (carotid sinus nerve and sciatic nerve) did not consistently elicit responses in the urinary bladder. However, stimulation of these afferents as well as pelvic nerve afferents evoked reflex firing in nerve filaments on the surface of the urinary bladder. The firing was abolished by transection of the ipsilateral hypogastric nerve. 5. It is concluded that stimulation of vesical afferents activates a spinal sympathetic reflex which results in closing of the internal urethral sphincter and a depression of bladder activity. The latter occurs by a direct depression of detrusor smooth muscle as well as a block of the neural input to the bladder. This vesico-sympathetic reflex represents a negative feed-back mechanism which may have an important role in the maintenance of urinary continence.", "PMID": 957214}
{"id": "pubmed23n0032_2613", "title": "Dynamic properties of the responses of single neurones in the cochlear nucleus of the rat.", "content": "1. The dynamic properties of unit responses to amplitude-modulated tones were studied using modulation with pseudorandom noise and described by cross-covariance and integrated cross-covariance functions between the discharge rate and the modulation. Under the experimental conditions used, these two functions are valid approximations of the system's impulse and step response functions respectively. 2. On the basis of their impulse response functions units could be classified into two groups, Type I with a low adaptation and Type II with a large degree of adaptation as well as a damped oscillation in their impulse response functions. 3. The response pattern of the Type II units is most likely the result of a negative feed-back striving to keep the discharge rate at a nearly constant level. 4. The cross-covariance functions are shown to remain unchanged during long duration recordings from the same unit.", "contents": "Dynamic properties of the responses of single neurones in the cochlear nucleus of the rat. 1. The dynamic properties of unit responses to amplitude-modulated tones were studied using modulation with pseudorandom noise and described by cross-covariance and integrated cross-covariance functions between the discharge rate and the modulation. Under the experimental conditions used, these two functions are valid approximations of the system's impulse and step response functions respectively. 2. On the basis of their impulse response functions units could be classified into two groups, Type I with a low adaptation and Type II with a large degree of adaptation as well as a damped oscillation in their impulse response functions. 3. The response pattern of the Type II units is most likely the result of a negative feed-back striving to keep the discharge rate at a nearly constant level. 4. The cross-covariance functions are shown to remain unchanged during long duration recordings from the same unit.", "PMID": 957247}
{"id": "pubmed23n0032_2614", "title": "Evidence for genetic control of glycine uptake in cultured cells, regulated by the amino acid concentration of the growth medium.", "content": "1. Cultured cells were grown in various concentrations of amino acids for periods up to 3 days and the characteristics of the glycine transport system measured under fixed experimental conditions. During this time, the effect of enucleation, using cytochalasin B, and the effects of protein synthesis inhibitors (cycloheximide and actinomycin D) were investigated. 2. Glycine influx is regulated by the prior growth concentration of similarly transported amino acids. 3. The modification in transport involves primarily a change in Vmax (but also a change in Km in HeLa cells) and is effected within 2-10 hr after media change. Increased transport activity is calculated to be sufficient to compensate for the reduction in extracellular amino acid concentration, so that nearly normal influx values from media are maintained. Regulation over the range of concentrations studied is shown to be very accurate. 4. The nucleus is essential for the regulatory mechanism to function. It seems probable that mRNA synthesis is required for acquisition of increased transport activity and mRNA translation required for maintenance of normal activity. 5. The controlling factor in the regulatory mechanism appears unlikely to be intracellular pool size. Other possible signals are discussed.", "contents": "Evidence for genetic control of glycine uptake in cultured cells, regulated by the amino acid concentration of the growth medium. 1. Cultured cells were grown in various concentrations of amino acids for periods up to 3 days and the characteristics of the glycine transport system measured under fixed experimental conditions. During this time, the effect of enucleation, using cytochalasin B, and the effects of protein synthesis inhibitors (cycloheximide and actinomycin D) were investigated. 2. Glycine influx is regulated by the prior growth concentration of similarly transported amino acids. 3. The modification in transport involves primarily a change in Vmax (but also a change in Km in HeLa cells) and is effected within 2-10 hr after media change. Increased transport activity is calculated to be sufficient to compensate for the reduction in extracellular amino acid concentration, so that nearly normal influx values from media are maintained. Regulation over the range of concentrations studied is shown to be very accurate. 4. The nucleus is essential for the regulatory mechanism to function. It seems probable that mRNA synthesis is required for acquisition of increased transport activity and mRNA translation required for maintenance of normal activity. 5. The controlling factor in the regulatory mechanism appears unlikely to be intracellular pool size. Other possible signals are discussed.", "PMID": 957248}
{"id": "pubmed23n0032_2615", "title": "Sensitivity of renin secretion to volume depletion in the anaesthetized dog: comparison between urinary drainage and slow haemorrhage.", "content": "1. An experimental technique utilizing 'denervation diuresis' from one kidney with measurement of renin release from the contralateral innervated kidney was developed to study the sensitivity of renin secretion to volume depletion. 2. With urine excretion, release of renin increased progressively from the innervated kidney. The increase was significant at a sodium deficit of 0-23 mole.kg-1. At a sodium deficit of 0-6 m-mole.kg-1 renin release had doubled. 3. Bilateral vagotomy did not alter this response. 4. Precise replacement of sodium loss with isotonic saline but without replacement of other urinary components returned renin release to control levels. 5. Slow haemorrhage causing a rate of volume and sodium loss equivalent to urinary drainage did not alter the rate of renin release. 6. With a single denervated kidney and contralateral nephrectomy, renin release fell progressively to minimal levels despite sodium deficits up to 2-6 m-mole.kg-1. 7. It is concluded that renin secretion is sensitive to at most a 0-5% change in body fluid volume and should be considered a primary response to volume depletion. The sensitivity of the response depends upon normal renal innervation but is not mediated via vascular volume receptors nor via receptors innervated by the vagus. 8. It is proposed that the extreme sensitivity of the renin-secreting system in these experiments results from the combination of volume depletion and slight hypotonicity of extracellular fluid acting on the renal afferent arteriole without the mediation of the macula densa,", "contents": "Sensitivity of renin secretion to volume depletion in the anaesthetized dog: comparison between urinary drainage and slow haemorrhage. 1. An experimental technique utilizing 'denervation diuresis' from one kidney with measurement of renin release from the contralateral innervated kidney was developed to study the sensitivity of renin secretion to volume depletion. 2. With urine excretion, release of renin increased progressively from the innervated kidney. The increase was significant at a sodium deficit of 0-23 mole.kg-1. At a sodium deficit of 0-6 m-mole.kg-1 renin release had doubled. 3. Bilateral vagotomy did not alter this response. 4. Precise replacement of sodium loss with isotonic saline but without replacement of other urinary components returned renin release to control levels. 5. Slow haemorrhage causing a rate of volume and sodium loss equivalent to urinary drainage did not alter the rate of renin release. 6. With a single denervated kidney and contralateral nephrectomy, renin release fell progressively to minimal levels despite sodium deficits up to 2-6 m-mole.kg-1. 7. It is concluded that renin secretion is sensitive to at most a 0-5% change in body fluid volume and should be considered a primary response to volume depletion. The sensitivity of the response depends upon normal renal innervation but is not mediated via vascular volume receptors nor via receptors innervated by the vagus. 8. It is proposed that the extreme sensitivity of the renin-secreting system in these experiments results from the combination of volume depletion and slight hypotonicity of extracellular fluid acting on the renal afferent arteriole without the mediation of the macula densa,", "PMID": 957249}
{"id": "pubmed23n0032_2616", "title": "Relationships between the spike components and the delayed depolarization in cat spinal neurones.", "content": "1. Changes in the delayed depolarization (DD) following composite (IS-SD) intracellular spikes in motoneurones and neurones of the ventral spinocerebellar tract were recorded in a variety of experimental conditions. Cell activation was either antidromic or by direct intracellular stimulation. 2. It was observed that under all conditions in which IS-SD coupling changes took place (as a consequence of spontaneous fluctuations, membrane conductance variations, variations of direct-stimulation parameters, changes in steady membrane polarization), SD spike delays were always accompanied by a progressive concomitant reduction of the DD depolarizing hump amplitude. 3. Under the same conditions the latency of the DD peak from the stimulus artifact remained constant. Accordingly, any increase of the SD delay was accompanied by a reciprocal reduction of the time interval between the SD spike and the DD peak. This variability of temporal relationships between SD spike and DD would appear to contradict the hypothesis that the DD might represent the image of the excitation spreading from the soma to the dendrites (Kernell, 1964; Nelson & Burke, 1967). 4. as the gradual reduction of the DD hump progressed, the time course of the decay phase of the afterhyperpotential more and more closely approximated the decay phase of the IS spike. As an alternative hypothesis it is suggested that the DD might originate from the current which generates the IS spike.", "contents": "Relationships between the spike components and the delayed depolarization in cat spinal neurones. 1. Changes in the delayed depolarization (DD) following composite (IS-SD) intracellular spikes in motoneurones and neurones of the ventral spinocerebellar tract were recorded in a variety of experimental conditions. Cell activation was either antidromic or by direct intracellular stimulation. 2. It was observed that under all conditions in which IS-SD coupling changes took place (as a consequence of spontaneous fluctuations, membrane conductance variations, variations of direct-stimulation parameters, changes in steady membrane polarization), SD spike delays were always accompanied by a progressive concomitant reduction of the DD depolarizing hump amplitude. 3. Under the same conditions the latency of the DD peak from the stimulus artifact remained constant. Accordingly, any increase of the SD delay was accompanied by a reciprocal reduction of the time interval between the SD spike and the DD peak. This variability of temporal relationships between SD spike and DD would appear to contradict the hypothesis that the DD might represent the image of the excitation spreading from the soma to the dendrites (Kernell, 1964; Nelson & Burke, 1967). 4. as the gradual reduction of the DD hump progressed, the time course of the decay phase of the afterhyperpotential more and more closely approximated the decay phase of the IS spike. As an alternative hypothesis it is suggested that the DD might originate from the current which generates the IS spike.", "PMID": 957250}
{"id": "pubmed23n0032_2617", "title": "Unit responses in the medulla oblongata of rabbit to changes in local and cutaneous temperature.", "content": "1. In anaesthetized rabbits which were stereotaxically implanted with a thermode following decerebellation, thermal sensitivity of neurones in the medulla oblongata to local temperature changes was examined systematically. The medullary temperature was changed by circulating water at various temperatures through the thermode. 2. Eighty-seven temperature-responsive neurones were found in the medulla, especially in the medullary reticular formation. Out of these eighty-seven, forty-eight neurones were cold-responsive neurones, while the rest were warm-responsive. Both types of neurones included not only neurones with linear or continuous relations between discharge rate and local temperature but also neurones with non-linear or discontinuous thermoresponse curves. 3. In the medulla a few of the temperature-responsive neurones showed a dynamic reaction with static discharge rate changes to changes of local temperature. 4. Seven neurones out of the fifteen respiratory neurones encountered during the exploration had thermal sensitivity to respiratory alteration of the discharge. 5. with thermal stimulation of a limited area of the skin, over 50% of the temperature-responsive neurones recorded in the medulla responded positively or negatively to changes in the skin temperature.", "contents": "Unit responses in the medulla oblongata of rabbit to changes in local and cutaneous temperature. 1. In anaesthetized rabbits which were stereotaxically implanted with a thermode following decerebellation, thermal sensitivity of neurones in the medulla oblongata to local temperature changes was examined systematically. The medullary temperature was changed by circulating water at various temperatures through the thermode. 2. Eighty-seven temperature-responsive neurones were found in the medulla, especially in the medullary reticular formation. Out of these eighty-seven, forty-eight neurones were cold-responsive neurones, while the rest were warm-responsive. Both types of neurones included not only neurones with linear or continuous relations between discharge rate and local temperature but also neurones with non-linear or discontinuous thermoresponse curves. 3. In the medulla a few of the temperature-responsive neurones showed a dynamic reaction with static discharge rate changes to changes of local temperature. 4. Seven neurones out of the fifteen respiratory neurones encountered during the exploration had thermal sensitivity to respiratory alteration of the discharge. 5. with thermal stimulation of a limited area of the skin, over 50% of the temperature-responsive neurones recorded in the medulla responded positively or negatively to changes in the skin temperature.", "PMID": 957251}
{"id": "pubmed23n0032_2618", "title": "Strain sensitivity and directionality in cat atrial mechanoreceptors in vitro.", "content": "1. Afferent discharges from single atrial mechanoreceptors were studied in vitro in isolated, perfused cat hearts, by balloon inflation and by localized endocardial stretching with step and ramp wave forms. 2. Receptor excitability could be maintained for several hours with perfusion, but failed reversibly within a half hour without it. 3. Responses to pure step stimuli were purely phasic, adapting within a few seconds as negative fractional powers of time. Ramp responses showed corresponding behaviour; 4. Median discharge sensitivities were 12 Hz ml.-1 for step balloon inflation and 110 Hz strain-1 for step local stretching, so that 1 ml. balloon expansion was equivalent to 10% local stretch. 5. Ramp stimulation indicated two distinct receptor groups, a 'high-pass latency' (HPL) group for which threshold decreased with increasing ramp velocity, and a larger 'low-pass latency' (LPL) group with the opposite behaviour. 6. The endocardial deformation due to local stretching comprised about half simple extension and half shear, so that half the effective lineal stretch would occur in end filaments oriented within 20 degrees of the stretch axis. 7. Of five receptors tested for directionality by local stretching in two perpendicular directions, four showed significant directionality in step sensitivity and/or ramp response latency, indicating the capability of receptive fields to encode changes in endocardial shape.", "contents": "Strain sensitivity and directionality in cat atrial mechanoreceptors in vitro. 1. Afferent discharges from single atrial mechanoreceptors were studied in vitro in isolated, perfused cat hearts, by balloon inflation and by localized endocardial stretching with step and ramp wave forms. 2. Receptor excitability could be maintained for several hours with perfusion, but failed reversibly within a half hour without it. 3. Responses to pure step stimuli were purely phasic, adapting within a few seconds as negative fractional powers of time. Ramp responses showed corresponding behaviour; 4. Median discharge sensitivities were 12 Hz ml.-1 for step balloon inflation and 110 Hz strain-1 for step local stretching, so that 1 ml. balloon expansion was equivalent to 10% local stretch. 5. Ramp stimulation indicated two distinct receptor groups, a 'high-pass latency' (HPL) group for which threshold decreased with increasing ramp velocity, and a larger 'low-pass latency' (LPL) group with the opposite behaviour. 6. The endocardial deformation due to local stretching comprised about half simple extension and half shear, so that half the effective lineal stretch would occur in end filaments oriented within 20 degrees of the stretch axis. 7. Of five receptors tested for directionality by local stretching in two perpendicular directions, four showed significant directionality in step sensitivity and/or ramp response latency, indicating the capability of receptive fields to encode changes in endocardial shape.", "PMID": 957252}
{"id": "pubmed23n0032_2619", "title": "An octopus toxin, maculotoxin, selectively blocks sodium current in squid axons.", "content": "1. A low molecular weight, stable, cationic neurotoxin (maculotoxin, MTX) extracted from the posterior salivary glands of the octopus Hapalochlaena maculosa, blocked sodium current in voltage-clamped squid axons without affecting potassium current. 2. The effectiveness of MTX was increased by repetitive, brief, depolarizing pulses but not by a single prolonged depolarization. 3. The potency of MTX decreased at pHs from 8 to 9. Effectiveness could be restored be restored by lowering the pH to 7-1 again. It was concluded that MTX is active in its cationic form. 4. MTX affected sodium conductance kinetics, slowing the turn-on of sodium current. This effect was most noticeable with small deploarizations but became progressively less with larger depolarizations. Neither the turn-off of sodium current nor sodium inactivation kinetics were affected by the toxin. 5. MTX inhibited sodium current without inhibiting sodium gating current. 6. The effectiveness of MTX was not detectably changed when calcium concentration was varied from 50 to 10 mM, or sodium concentration was varied from 225 to 750 mM.", "contents": "An octopus toxin, maculotoxin, selectively blocks sodium current in squid axons. 1. A low molecular weight, stable, cationic neurotoxin (maculotoxin, MTX) extracted from the posterior salivary glands of the octopus Hapalochlaena maculosa, blocked sodium current in voltage-clamped squid axons without affecting potassium current. 2. The effectiveness of MTX was increased by repetitive, brief, depolarizing pulses but not by a single prolonged depolarization. 3. The potency of MTX decreased at pHs from 8 to 9. Effectiveness could be restored be restored by lowering the pH to 7-1 again. It was concluded that MTX is active in its cationic form. 4. MTX affected sodium conductance kinetics, slowing the turn-on of sodium current. This effect was most noticeable with small deploarizations but became progressively less with larger depolarizations. Neither the turn-off of sodium current nor sodium inactivation kinetics were affected by the toxin. 5. MTX inhibited sodium current without inhibiting sodium gating current. 6. The effectiveness of MTX was not detectably changed when calcium concentration was varied from 50 to 10 mM, or sodium concentration was varied from 225 to 750 mM.", "PMID": 957253}
{"id": "pubmed23n0032_2620", "title": "The dark adaptation curve of rods measured by their after-image.", "content": "1. The common dark adaptation curve exhibits two branches; the course of the rod branch cannot normally be measured at early times since it lies above the observed cone thresholds. In this paper we measure it. 2. this is done by observing the negative after-image against a uniform background critically adjusted in luminance. 3. adjacent to the bleached area to be studied is a second area more strongly bleached. If the background intensity is below threshold for the less bleached area it will not be seen there; but if the background is above that threshold, this area will be seen brighter than the other. 4. the dark adapted threshold on the less bleached area is therefore the background luminance which just permits the two areas to be distinguished in the after-image. 5. after 5 min cones have quite recovered, and thus have no after-image to contaminate the rod image. 6. the rod curve measured by after-image is traced over 5 units of log threshold: it is an exponential with half life of 4.5 min, and coincides with the time course of regeneration of rhodopsin in man.", "contents": "The dark adaptation curve of rods measured by their after-image. 1. The common dark adaptation curve exhibits two branches; the course of the rod branch cannot normally be measured at early times since it lies above the observed cone thresholds. In this paper we measure it. 2. this is done by observing the negative after-image against a uniform background critically adjusted in luminance. 3. adjacent to the bleached area to be studied is a second area more strongly bleached. If the background intensity is below threshold for the less bleached area it will not be seen there; but if the background is above that threshold, this area will be seen brighter than the other. 4. the dark adapted threshold on the less bleached area is therefore the background luminance which just permits the two areas to be distinguished in the after-image. 5. after 5 min cones have quite recovered, and thus have no after-image to contaminate the rod image. 6. the rod curve measured by after-image is traced over 5 units of log threshold: it is an exponential with half life of 4.5 min, and coincides with the time course of regeneration of rhodopsin in man.", "PMID": 957254}
{"id": "pubmed23n0032_2621", "title": "Inhibition of food intake in the rat following complete absorption of glucose delivered into the stomach, intestine or liver.", "content": "1. Solutions of glucose or other carbohydrates were administered during the dark or light period of the circadian cycle to rats which had been only briefly deprived of food. 2. food was restored to the animals at various times after administration of a glucose load by stomach tube. With delays between loading and access to food of up to 3 hr by night and 2 hr by day, subsequent food intake was less than intake after non-nutritive loads. 3. measurement of the glucose content of the gastrointestinal tract at various times after glucose loading showed that this depression of intake was still apparent even when the rat was offered food some time after complete absorption of the stomach load. 4. infusion of a glucose solution into the duodenum or the hepatic protal vein also inhibited subsequent food intake. 5. in all cases, the inhibition of food intake was expressed as a decrease in the size of the first meal after restoring access to food. 6. these results provide the first demonstration that the entry of normal amounts of carbohydrate into the body by the physiological route is followed by depression of food intake which lasts until after absorption is complete.", "contents": "Inhibition of food intake in the rat following complete absorption of glucose delivered into the stomach, intestine or liver. 1. Solutions of glucose or other carbohydrates were administered during the dark or light period of the circadian cycle to rats which had been only briefly deprived of food. 2. food was restored to the animals at various times after administration of a glucose load by stomach tube. With delays between loading and access to food of up to 3 hr by night and 2 hr by day, subsequent food intake was less than intake after non-nutritive loads. 3. measurement of the glucose content of the gastrointestinal tract at various times after glucose loading showed that this depression of intake was still apparent even when the rat was offered food some time after complete absorption of the stomach load. 4. infusion of a glucose solution into the duodenum or the hepatic protal vein also inhibited subsequent food intake. 5. in all cases, the inhibition of food intake was expressed as a decrease in the size of the first meal after restoring access to food. 6. these results provide the first demonstration that the entry of normal amounts of carbohydrate into the body by the physiological route is followed by depression of food intake which lasts until after absorption is complete.", "PMID": 957255}
{"id": "pubmed23n0032_2622", "title": "Respiratory modulation of barareceptor and chemoreceptor reflexes affecting heart rate and cardiac vagal efferent nerve activity.", "content": "1. Brief stimuli were delivered to the carotid chemoreceptors or baroreceptors in dogs anaesthetized with chloralose. Chemoreceptor stimulation was achieved by rapid retrograde injection of 0.2-0.5 ml. CO2 equilibrated saline through a cannula in the external carotid artery. Baroreceptor stimulation was achieved by forceful retrograde injection of 2-5 ml. air-equilibrated saline into the external carotid artery after first clamping the common carotid artery. 2. prompt decreases in heart rate were elicited by brief sudden chemoreceptor or baroreceptor stimuli when these were delivered during the expiratory phase of respiration. The stimuli did not modify the control heart rate pattern when delivered in the inspiratory phase of respiration. This respiratory modulation of reflex effectiveness persisted when the animals were completely paralysed and the phase of the respiratory cycle was monitored through a phrenic electroneurogram. 3. single cardiac vagal efferent nerve fibres were dissected from the cut central end of the right cervical vagus nerve. They were classified as cardiac efferents by their cardiac and respiratory rhythmicity, and by their increased activity in response to stimulation of a carotid sinus nerve or to mechanical elevation of the systemic arterial pressure. These efferent fibres increased their activity in response to brief chemoreceptor or baroreceptor stimuli delivered in expiration, but did not respond to stimuli delivered in inspiration. This respiratory modulation of both reflexes persisted after bilateral cervical vagotomy.", "contents": "Respiratory modulation of barareceptor and chemoreceptor reflexes affecting heart rate and cardiac vagal efferent nerve activity. 1. Brief stimuli were delivered to the carotid chemoreceptors or baroreceptors in dogs anaesthetized with chloralose. Chemoreceptor stimulation was achieved by rapid retrograde injection of 0.2-0.5 ml. CO2 equilibrated saline through a cannula in the external carotid artery. Baroreceptor stimulation was achieved by forceful retrograde injection of 2-5 ml. air-equilibrated saline into the external carotid artery after first clamping the common carotid artery. 2. prompt decreases in heart rate were elicited by brief sudden chemoreceptor or baroreceptor stimuli when these were delivered during the expiratory phase of respiration. The stimuli did not modify the control heart rate pattern when delivered in the inspiratory phase of respiration. This respiratory modulation of reflex effectiveness persisted when the animals were completely paralysed and the phase of the respiratory cycle was monitored through a phrenic electroneurogram. 3. single cardiac vagal efferent nerve fibres were dissected from the cut central end of the right cervical vagus nerve. They were classified as cardiac efferents by their cardiac and respiratory rhythmicity, and by their increased activity in response to stimulation of a carotid sinus nerve or to mechanical elevation of the systemic arterial pressure. These efferent fibres increased their activity in response to brief chemoreceptor or baroreceptor stimuli delivered in expiration, but did not respond to stimuli delivered in inspiration. This respiratory modulation of both reflexes persisted after bilateral cervical vagotomy.", "PMID": 957256}
{"id": "pubmed23n0032_2623", "title": "Stretch reflex and servo action in a variety of human muscles.", "content": "1. In the long flexor of the thumb the latency of the stretch reflex and of other manifestations of servo action is some 45 msec, roughly double the latency of a finger jerk. 2. Tendon jerks are feeble or absent in the long flexor of the thumb even in subjects with brisk long-latency stretch reflexes in this muscle. This, and other facts, suggests that the nervous mechanism of the tendon jerk is different from that of the stretch reflex. 3. A muscle that has feeble tendon jerks may show a late component in the response to a tendon tap, with a latency similar to that of the long-latency stretch reflex. 4. On the hypothesis that the excess latency of the stretch reflex over that of a tendon jerk is because the stretch reflex employs a cortical rather than a spinal arc, the excess would be expected to be larger in magnitude for the long flexor of the big toe and smaller for the jaw closing muscles. This is confirmed, 5. An alternative hypothesis that the long latency of stretch reflexes in thumb and toe is because they are excited by slow-conducting afferents is made improbable by the finding that stretch reflexes with an equal or greater excess latency are also found in proximal arm muscles. 6. The long-latency stretch reflex in proximal muscles was seen most distinctly in a healthy subject who happened to have feeble or absent tendon jerks. In ordinary subjects there is often a large, short-latency, presumably spinal component of the stretch reflex in proximal muscles; and short-latency responses to halt and release are also seen, The significance of this spinal latency servo action in proximal muscles remains to be explored. 7. The Discussion argues that the available data on conduction time to and from the cerebral cortex are compatible with the hypothesis that the long-latency component of the stretch reflex uses a transcortical reflex arc, and that none of the experiments described in the present paper are inimical to this view.", "contents": "Stretch reflex and servo action in a variety of human muscles. 1. In the long flexor of the thumb the latency of the stretch reflex and of other manifestations of servo action is some 45 msec, roughly double the latency of a finger jerk. 2. Tendon jerks are feeble or absent in the long flexor of the thumb even in subjects with brisk long-latency stretch reflexes in this muscle. This, and other facts, suggests that the nervous mechanism of the tendon jerk is different from that of the stretch reflex. 3. A muscle that has feeble tendon jerks may show a late component in the response to a tendon tap, with a latency similar to that of the long-latency stretch reflex. 4. On the hypothesis that the excess latency of the stretch reflex over that of a tendon jerk is because the stretch reflex employs a cortical rather than a spinal arc, the excess would be expected to be larger in magnitude for the long flexor of the big toe and smaller for the jaw closing muscles. This is confirmed, 5. An alternative hypothesis that the long latency of stretch reflexes in thumb and toe is because they are excited by slow-conducting afferents is made improbable by the finding that stretch reflexes with an equal or greater excess latency are also found in proximal arm muscles. 6. The long-latency stretch reflex in proximal muscles was seen most distinctly in a healthy subject who happened to have feeble or absent tendon jerks. In ordinary subjects there is often a large, short-latency, presumably spinal component of the stretch reflex in proximal muscles; and short-latency responses to halt and release are also seen, The significance of this spinal latency servo action in proximal muscles remains to be explored. 7. The Discussion argues that the available data on conduction time to and from the cerebral cortex are compatible with the hypothesis that the long-latency component of the stretch reflex uses a transcortical reflex arc, and that none of the experiments described in the present paper are inimical to this view.", "PMID": 957257}
{"id": "pubmed23n0032_2624", "title": "Respiratory and cardiovascular interactions in ducks: the effect of lung denervation on the initation of and recovery from some cardiovascular responses to submergence.", "content": "Lung denervation in ducks, by sectioning all vagal branches to one lung following mid-cervical vagotomy on the other side, resulted in immediate bradycardia and fall in breathing frequency. Some 3-5 weeks after lung denervation breathing frequency was within the normal range but the lung inflation reflex, present in unilaterally vagotomized sham-operated ducks, was abolished. During 2 min dives there were no significant differences between sham-operated and denervated ducks in heart rate, arterial blood pressure, blood gas tensions and pH(a). However, during recovery from diving heart rate increased more slowly in denervates and breathing rate was significantly below that attained by shams, although tidal volume rose to a maximum increase of 139% to a maximum of 225% of the pre-dive value in denervates in contrast to a maximum increase of 139% of pre-dive in sham-operated ducks. Both sham-operated and denervated ducks exhibited a significant fall in diastolic blood pressure 60 sec after emergence...", "contents": "Respiratory and cardiovascular interactions in ducks: the effect of lung denervation on the initation of and recovery from some cardiovascular responses to submergence. Lung denervation in ducks, by sectioning all vagal branches to one lung following mid-cervical vagotomy on the other side, resulted in immediate bradycardia and fall in breathing frequency. Some 3-5 weeks after lung denervation breathing frequency was within the normal range but the lung inflation reflex, present in unilaterally vagotomized sham-operated ducks, was abolished. During 2 min dives there were no significant differences between sham-operated and denervated ducks in heart rate, arterial blood pressure, blood gas tensions and pH(a). However, during recovery from diving heart rate increased more slowly in denervates and breathing rate was significantly below that attained by shams, although tidal volume rose to a maximum increase of 139% to a maximum of 225% of the pre-dive value in denervates in contrast to a maximum increase of 139% of pre-dive in sham-operated ducks. Both sham-operated and denervated ducks exhibited a significant fall in diastolic blood pressure 60 sec after emergence...", "PMID": 957258}
{"id": "pubmed23n0032_2625", "title": "Calcium conductance in relation to contractility in frog myocardium.", "content": "1. Ca inward current and the corresponding phasic component of tension were measured in frog atrial muscle under voltage-clamp conditions in Na-free (Li) Ringer solution with tetrodotoxin (TTX) added. 2. The quantity of Ca ions entering the cell upon depolarization, delta[Ca]i, was linearly related to peak phasic tension. 3. The voltage dependence of the steady-state inactivation of the Ca-carrying system, f infinity, against voltage yielded similar relationships whether determined directly from variations of Ca inward current or peak phasic tension. The Ca system was almost fully available at potentials more negative than -45 mV and almost fully inactivated at potentials more positive than +10 mV. 4. It was established that the time- and voltage-dependence of Ca current and of phasic tension are directly related. The time constants of Ca activation, tau f, were comparable in the range of membrane potential investigated (-20 to +25 mV), whether determined directly from the decay of Ca current or indirectly from peak phasic tension. 5. It was concluded that the Ca current, ICa, directly activates phasic contraction and that either parameter can be used as an indicator of the kinetics of the Ca-carrying system. Peak phasic tension was used to determine tau f further in the membrane potential range in which interference by other membrane currents renders direct analysis of Ca current difficult. 6. The tau f against voltage relationship determined from phasic tension showed that the inactivation process of the Ca-carrying system is slowest at membrane potentials around -13 mV (tau f = 55 msec) and that the rate of inactivation increases with both increasing and decreasing depolarizations. 7. It is suggested that normal repolarization in frog myocardium depends mainly on the decay of Ca inward current rather than on an increase of outward current.", "contents": "Calcium conductance in relation to contractility in frog myocardium. 1. Ca inward current and the corresponding phasic component of tension were measured in frog atrial muscle under voltage-clamp conditions in Na-free (Li) Ringer solution with tetrodotoxin (TTX) added. 2. The quantity of Ca ions entering the cell upon depolarization, delta[Ca]i, was linearly related to peak phasic tension. 3. The voltage dependence of the steady-state inactivation of the Ca-carrying system, f infinity, against voltage yielded similar relationships whether determined directly from variations of Ca inward current or peak phasic tension. The Ca system was almost fully available at potentials more negative than -45 mV and almost fully inactivated at potentials more positive than +10 mV. 4. It was established that the time- and voltage-dependence of Ca current and of phasic tension are directly related. The time constants of Ca activation, tau f, were comparable in the range of membrane potential investigated (-20 to +25 mV), whether determined directly from the decay of Ca current or indirectly from peak phasic tension. 5. It was concluded that the Ca current, ICa, directly activates phasic contraction and that either parameter can be used as an indicator of the kinetics of the Ca-carrying system. Peak phasic tension was used to determine tau f further in the membrane potential range in which interference by other membrane currents renders direct analysis of Ca current difficult. 6. The tau f against voltage relationship determined from phasic tension showed that the inactivation process of the Ca-carrying system is slowest at membrane potentials around -13 mV (tau f = 55 msec) and that the rate of inactivation increases with both increasing and decreasing depolarizations. 7. It is suggested that normal repolarization in frog myocardium depends mainly on the decay of Ca inward current rather than on an increase of outward current.", "PMID": 957259}
{"id": "pubmed23n0032_2626", "title": "Calcium-sodium antagonism on the frog's heart: a voltage-clamp study.", "content": "1. In double sucrose-gap voltage-clamped frog atrial fibres the influence of [Ca]o and [Na]o on membrane current and contraction was investigated. 2. The slow (secondary) inward current varied with [Ca]o but was almost insensitive to changes in [Na]o. In contrast, the phasic (transient) contraction initiated by the slow inward current was affected by both [Ca]o and [Na]o. 3. With moderate changes of [Ca]o and [Na]o from normal, the strength of phasic contraction at a given depolarization followed the [Ca]o/[Na]2o ratio approximately. This was best seen at membrane potentials near zero level. 4. Under the same conditions, tonic (sustained) contractions associated with prolonged depolarizations were strictly correlated to the [Ca]o/[Na]2o ratio at any potential. No interrelation between tonic tension and steady-state current was found. 5. With extensive changes in [Ca]o and [Na]o, the sensitivity of both phasic and tonic tension to the [Ca]o/[Na]2o ratio declined, the negative effect of [Na]o becoming smaller than was expected from this ratio. 6. In Na-free choline-Ringer, a strong contracture developed followed by a spontaneous relaxation. Starting from the relaxed state, application of depolarizing clamps gave rise to phasic contractions with a very slow relaxation while tonic contractions were apparently lacking. 7. The results are interpreted in terms of an energy-dependent carrier mechanism exchanging one Ca for two Na ions across the cell membrane. The model implies a strong asymmetry in the rate constants governing the chemical reactions on both sides of the membrane. The system is thought to operate close to equilibrium at any potential, thereby determining the steady level of myoplasmic Ca. The equilibrium itself is considered to shift upon depolarization. Assuming that [Na]i is constant, the steady level of [Ca]i is expected to be proportional to the [Ca]o/[Na]2o ratio, the scale factor being a function of membrane potential. 8. The carrier model suggests the occurrence of a depolarization-induced inward transfer of Ca which might be involved in the generation of tonic contractions. 9. The apparent lack of tonic contractions in the absence of external Na ions may be explained by a suppression of carrier-mediated Ca influx normally occurring upon depolarization. 10. The antagonistic effects of [Ca]o and [Na]o on phasic contraction are understood as being due to alterations of the Ca pumping system rather than changes in slow inward current.", "contents": "Calcium-sodium antagonism on the frog's heart: a voltage-clamp study. 1. In double sucrose-gap voltage-clamped frog atrial fibres the influence of [Ca]o and [Na]o on membrane current and contraction was investigated. 2. The slow (secondary) inward current varied with [Ca]o but was almost insensitive to changes in [Na]o. In contrast, the phasic (transient) contraction initiated by the slow inward current was affected by both [Ca]o and [Na]o. 3. With moderate changes of [Ca]o and [Na]o from normal, the strength of phasic contraction at a given depolarization followed the [Ca]o/[Na]2o ratio approximately. This was best seen at membrane potentials near zero level. 4. Under the same conditions, tonic (sustained) contractions associated with prolonged depolarizations were strictly correlated to the [Ca]o/[Na]2o ratio at any potential. No interrelation between tonic tension and steady-state current was found. 5. With extensive changes in [Ca]o and [Na]o, the sensitivity of both phasic and tonic tension to the [Ca]o/[Na]2o ratio declined, the negative effect of [Na]o becoming smaller than was expected from this ratio. 6. In Na-free choline-Ringer, a strong contracture developed followed by a spontaneous relaxation. Starting from the relaxed state, application of depolarizing clamps gave rise to phasic contractions with a very slow relaxation while tonic contractions were apparently lacking. 7. The results are interpreted in terms of an energy-dependent carrier mechanism exchanging one Ca for two Na ions across the cell membrane. The model implies a strong asymmetry in the rate constants governing the chemical reactions on both sides of the membrane. The system is thought to operate close to equilibrium at any potential, thereby determining the steady level of myoplasmic Ca. The equilibrium itself is considered to shift upon depolarization. Assuming that [Na]i is constant, the steady level of [Ca]i is expected to be proportional to the [Ca]o/[Na]2o ratio, the scale factor being a function of membrane potential. 8. The carrier model suggests the occurrence of a depolarization-induced inward transfer of Ca which might be involved in the generation of tonic contractions. 9. The apparent lack of tonic contractions in the absence of external Na ions may be explained by a suppression of carrier-mediated Ca influx normally occurring upon depolarization. 10. The antagonistic effects of [Ca]o and [Na]o on phasic contraction are understood as being due to alterations of the Ca pumping system rather than changes in slow inward current.", "PMID": 957260}
{"id": "pubmed23n0032_2627", "title": "Threshold setting by the surround of cat retinal ganglion cells.", "content": "1. The slope of curves relating the log increment threshold to log background luminance in cat retinal ganglion cells is affected by the area and duration of the test stimulus, as it is in human pyschophysical experiments. 2. Using large area, long duration stimuli the slopes average 0-82 and approach close to 1 (Weber's Law) in the steepest cases. Small stimuli gave an average of 0-53 for on-centre units using brief stimuli, and 0-56 for off-centre units, using long stimuli. Slopes under 0-5 (square root law) were not found over an extended range of luminances. 3. On individual units the slope was generally greater for larger and longer test stimulus, but no unit showed the full extent of change from slope of 0-5 to slope of 1. 4. The above differences hold for objective measures of quantum/spike ratio, as well as for thresholds either judged by ear or assessed by calculation. 5. The steeper slope of the curves for large area, long duration test stimuli compared with small, long duration stimuli, is associated with the increased effectiveness of antagonism from the surround at high backgrounds. This change may be less pronounced in off-centre units, one of which (probably transient Y-type) showed no difference of slope, and gave parallel area-threshold curves at widely separated background luminances, confirming the importance of differential surround effectiveness in changing the slope of the curves. 6. In on-centre units, the increased relative effectiveness of the surround is associated with the part of the raised background light that falls on the receptive field centre. 7. It is suggested that the variable surround functions as a zero-offset control that sets the threshold excitation required for generating impulses, and that this is separate from gain-setting adaptive mechanisms. This may be how ganglion cells maintain high incremental sensitivity in spite of a strong maintained excitatory drive that would otherwise cause compressive response non-linearities.", "contents": "Threshold setting by the surround of cat retinal ganglion cells. 1. The slope of curves relating the log increment threshold to log background luminance in cat retinal ganglion cells is affected by the area and duration of the test stimulus, as it is in human pyschophysical experiments. 2. Using large area, long duration stimuli the slopes average 0-82 and approach close to 1 (Weber's Law) in the steepest cases. Small stimuli gave an average of 0-53 for on-centre units using brief stimuli, and 0-56 for off-centre units, using long stimuli. Slopes under 0-5 (square root law) were not found over an extended range of luminances. 3. On individual units the slope was generally greater for larger and longer test stimulus, but no unit showed the full extent of change from slope of 0-5 to slope of 1. 4. The above differences hold for objective measures of quantum/spike ratio, as well as for thresholds either judged by ear or assessed by calculation. 5. The steeper slope of the curves for large area, long duration test stimuli compared with small, long duration stimuli, is associated with the increased effectiveness of antagonism from the surround at high backgrounds. This change may be less pronounced in off-centre units, one of which (probably transient Y-type) showed no difference of slope, and gave parallel area-threshold curves at widely separated background luminances, confirming the importance of differential surround effectiveness in changing the slope of the curves. 6. In on-centre units, the increased relative effectiveness of the surround is associated with the part of the raised background light that falls on the receptive field centre. 7. It is suggested that the variable surround functions as a zero-offset control that sets the threshold excitation required for generating impulses, and that this is separate from gain-setting adaptive mechanisms. This may be how ganglion cells maintain high incremental sensitivity in spite of a strong maintained excitatory drive that would otherwise cause compressive response non-linearities.", "PMID": 957261}
{"id": "pubmed23n0032_2628", "title": "Lithium transport by the colon of normal and sodium-depleted rats.", "content": "1. The transport of Li by colonic epithelium has been examined in normal and Na-depleted rats. 2. Substitution of Li for Na with lumen of the conon causes the transepithelial electrical potential difference (p.d.) and short-circuit current to fall to low levels and the electrical resistance of fall moderately. Recovery occurs by fairly slowly after removal of Li. 3. Li absorption increases linearly with increasing concentration in the lumen and is significantly faster in Na-depleted rats. Increasing the luminal Na concentration reduces Li absorption from solutions of low Li concentration. 4. Comparison of absorption rates with secretion rates in rats given Li systemically, together with measurements of Li distribution across the epithelium in relationship to the transepithelial p.d. indicate that Li transport is predominatly or entirely passive. Interference with Li absorption by Na suggests, however a mucosal membrane carrier which, since Li absorption rises after Na depletion, may be increased in the Na-depleted state.", "contents": "Lithium transport by the colon of normal and sodium-depleted rats. 1. The transport of Li by colonic epithelium has been examined in normal and Na-depleted rats. 2. Substitution of Li for Na with lumen of the conon causes the transepithelial electrical potential difference (p.d.) and short-circuit current to fall to low levels and the electrical resistance of fall moderately. Recovery occurs by fairly slowly after removal of Li. 3. Li absorption increases linearly with increasing concentration in the lumen and is significantly faster in Na-depleted rats. Increasing the luminal Na concentration reduces Li absorption from solutions of low Li concentration. 4. Comparison of absorption rates with secretion rates in rats given Li systemically, together with measurements of Li distribution across the epithelium in relationship to the transepithelial p.d. indicate that Li transport is predominatly or entirely passive. Interference with Li absorption by Na suggests, however a mucosal membrane carrier which, since Li absorption rises after Na depletion, may be increased in the Na-depleted state.", "PMID": 957262}
{"id": "pubmed23n0032_2629", "title": "The effect of lithium on the transport of sodium, potassium and chloride by the colon of normal and sodium-depleted rats.", "content": "1. The effect of Li, given systemically or placed in the gut lumen, on the transport of Na, K and C1 and on the transepithelial electrical potential difference (p.d.) was studied in vivo in the distal colon of normal and Na-depleted rats. 2. The specific effect of Li appeared to be on the Na transport system with K and C1 transport affected only indirectly. Active Na absorption was impaired and p.d. reduced when either Li was in the lumen or given systemically. In addition with Li in the lumen, a considerable rise in the plasma-to-lumen Na flux was observed, the flux increasing progressively with rising intraluminal Li concentration. The effects were greater in Na-depleted rats. 3. The greater part of Li absorption from the colon of the rat takes place by exchange for Na, the secretion of which is much enhanced while the p.d. is reduced. This contrasts with human colon in which potassium is the ion exchanged for Li with the p.d. increased.", "contents": "The effect of lithium on the transport of sodium, potassium and chloride by the colon of normal and sodium-depleted rats. 1. The effect of Li, given systemically or placed in the gut lumen, on the transport of Na, K and C1 and on the transepithelial electrical potential difference (p.d.) was studied in vivo in the distal colon of normal and Na-depleted rats. 2. The specific effect of Li appeared to be on the Na transport system with K and C1 transport affected only indirectly. Active Na absorption was impaired and p.d. reduced when either Li was in the lumen or given systemically. In addition with Li in the lumen, a considerable rise in the plasma-to-lumen Na flux was observed, the flux increasing progressively with rising intraluminal Li concentration. The effects were greater in Na-depleted rats. 3. The greater part of Li absorption from the colon of the rat takes place by exchange for Na, the secretion of which is much enhanced while the p.d. is reduced. This contrasts with human colon in which potassium is the ion exchanged for Li with the p.d. increased.", "PMID": 957263}
{"id": "pubmed23n0032_2630", "title": "Effects of antrectomy or porta-caval shunting on the histamine-storing endocrine-like cells in oxyntic mucosa of rat stomach. A fluorescence histochemical, electron microscopic and chemical study.", "content": "1. The argyrophil (enterochromaffin-like) cells in the oxyntic gland area of the rat stomach contain histamine, which can be demonstrated fluorescence microscopically after exposure to gaseous OPT. After administration of L-dopa (or L-5-hydroxytryptophan), these cells produce and temporarily store dopamine (or 5-hydroxytryptamine), demonstrable by its characteristic formaldehyde-induced fluorescence. Ultrastructurally, the enterochromaffin-like cells, which have the appearance of polypeptide hormone-secreting cells, comprise two main cell types, the most predominant one having vesicular type granules (EGL cells), the second most predominant one having smaller, uniformly electron dense granules (A-like cells). 2. Rats were subjected to the following surgical treatments: antrectomy; porta-caval shunting; antrectomy+porta-caval shunting; or sham-operation. Three to eight weeks after surgery the histamine-storing cells (enterochromaffin-like cells) of the oxyntic mucosa were analysed by fluorescence histochemistry, light and (quantitative) electron microscopy, and fluorometric determination of amines. 3. After antrectomy, fluorescence histochemistry and silver staining revealed a reduced number of enterochromaffin-like cells. The histamine content in the oxyntic mucosa was reduced by about 50%. As in unoperated injection of pentagastrin seemed to mobilize histamine. Feeding or injection of insulin failed to do so in antrectomized as opposed to control rats. Ultrastructurally, the cytoplasmic granules of both endocrine-like cell types were less numerous than in the unoperated rats. The reduction in cell number and granularity was particularly conspicuous with regard to the EGL cells. 4. After porta-caval shunting the number of enterochromaffin-like cells increased markedly. Chemical determination revealed a twofold increase in the histamine concentration of the oxyntic mucosa. Feeding or injection of insulin or pentagastrin lowered the histamine concentration. As judged by electron microscopy, the proliferation of endocrine-like cells induced by porta-caval shunting was restricted to the ECL cell type. Besides occurring in greater number, these cells were larger than those in unoperated controls, and their cytoplasm was densely packed with granules that were increased in size. 5. Following antrectomy of the porta-caval shunted rats the number of enterochromaffin-like cells and the oxyntic histamine concentration was reduced. 6. The results support the idea that gastrin exerts trophic as well as excitatory effects on oxyntic endocrine-like cells.", "contents": "Effects of antrectomy or porta-caval shunting on the histamine-storing endocrine-like cells in oxyntic mucosa of rat stomach. A fluorescence histochemical, electron microscopic and chemical study. 1. The argyrophil (enterochromaffin-like) cells in the oxyntic gland area of the rat stomach contain histamine, which can be demonstrated fluorescence microscopically after exposure to gaseous OPT. After administration of L-dopa (or L-5-hydroxytryptophan), these cells produce and temporarily store dopamine (or 5-hydroxytryptamine), demonstrable by its characteristic formaldehyde-induced fluorescence. Ultrastructurally, the enterochromaffin-like cells, which have the appearance of polypeptide hormone-secreting cells, comprise two main cell types, the most predominant one having vesicular type granules (EGL cells), the second most predominant one having smaller, uniformly electron dense granules (A-like cells). 2. Rats were subjected to the following surgical treatments: antrectomy; porta-caval shunting; antrectomy+porta-caval shunting; or sham-operation. Three to eight weeks after surgery the histamine-storing cells (enterochromaffin-like cells) of the oxyntic mucosa were analysed by fluorescence histochemistry, light and (quantitative) electron microscopy, and fluorometric determination of amines. 3. After antrectomy, fluorescence histochemistry and silver staining revealed a reduced number of enterochromaffin-like cells. The histamine content in the oxyntic mucosa was reduced by about 50%. As in unoperated injection of pentagastrin seemed to mobilize histamine. Feeding or injection of insulin failed to do so in antrectomized as opposed to control rats. Ultrastructurally, the cytoplasmic granules of both endocrine-like cell types were less numerous than in the unoperated rats. The reduction in cell number and granularity was particularly conspicuous with regard to the EGL cells. 4. After porta-caval shunting the number of enterochromaffin-like cells increased markedly. Chemical determination revealed a twofold increase in the histamine concentration of the oxyntic mucosa. Feeding or injection of insulin or pentagastrin lowered the histamine concentration. As judged by electron microscopy, the proliferation of endocrine-like cells induced by porta-caval shunting was restricted to the ECL cell type. Besides occurring in greater number, these cells were larger than those in unoperated controls, and their cytoplasm was densely packed with granules that were increased in size. 5. Following antrectomy of the porta-caval shunted rats the number of enterochromaffin-like cells and the oxyntic histamine concentration was reduced. 6. The results support the idea that gastrin exerts trophic as well as excitatory effects on oxyntic endocrine-like cells.", "PMID": 957264}
{"id": "pubmed23n0032_2631", "title": "Functional adaptation of sarcomere number of normal cat muscle.", "content": "1. Physiological and histological data were obtained from soleus and tibialis anterior muscles of normal adult cats to study to what extent fibre length and sarcomere number varied between animals and how this was related to the physiological characteristics of the muscles. 2. For a given muscle, the variation in the sarcomere number of individual muscle fibres between animals was found to be about 25%. These difference could partly be explained by comparing the number of sarcomeres and the length of the fibula, which was chosen as an index of the size of the animal. The average sarcomere number in the tibialis anterior muscle was about 60% greater than in the soleus. The variations between animals and between the anterior tibialis and soleus muscles were significantly greater than the variations observed within the same muscle. 3. The sarcomere length is dependent upon the articular angle, that is to say, the length change imposed on the muscle. These length changes are more extensive in the soleus muscle (100%) than in the tibialis anterior muscle (60%). A very significant correlation was found between articular angle and sarcomere length (for soleus r = 0.98, for tibialis anterior r = 0.94). 4. Although fibre length did vary considerably within a given muscle, the sarcomere length showed only minor variations at any given angle. This suggests that sarcomere number is determined in each individual muscle fibre. Such an adaptation implies a concomitant adaptation of the tendinous part of the fibre. This adaptation, resulting in definite sarcomere length at a definite angle, has obvious physiological implications. 5. Conventional length vs. active tension curves were established for the soleus and the tibialis anterior muscles. It is suggested that the difference between the sarcomere number of the two muscles may result in the difference between the shape of curves of these two muscles. 6. Active torque-angle curves were established for the two muscles in situ. The shapes of the curves for soleus and tibialis anterior are similar in spite of the different mechanical conditions of the two muscles. This fact helps to explain why the two muscles, despite their similar articular range, had very different sarcomere number.", "contents": "Functional adaptation of sarcomere number of normal cat muscle. 1. Physiological and histological data were obtained from soleus and tibialis anterior muscles of normal adult cats to study to what extent fibre length and sarcomere number varied between animals and how this was related to the physiological characteristics of the muscles. 2. For a given muscle, the variation in the sarcomere number of individual muscle fibres between animals was found to be about 25%. These difference could partly be explained by comparing the number of sarcomeres and the length of the fibula, which was chosen as an index of the size of the animal. The average sarcomere number in the tibialis anterior muscle was about 60% greater than in the soleus. The variations between animals and between the anterior tibialis and soleus muscles were significantly greater than the variations observed within the same muscle. 3. The sarcomere length is dependent upon the articular angle, that is to say, the length change imposed on the muscle. These length changes are more extensive in the soleus muscle (100%) than in the tibialis anterior muscle (60%). A very significant correlation was found between articular angle and sarcomere length (for soleus r = 0.98, for tibialis anterior r = 0.94). 4. Although fibre length did vary considerably within a given muscle, the sarcomere length showed only minor variations at any given angle. This suggests that sarcomere number is determined in each individual muscle fibre. Such an adaptation implies a concomitant adaptation of the tendinous part of the fibre. This adaptation, resulting in definite sarcomere length at a definite angle, has obvious physiological implications. 5. Conventional length vs. active tension curves were established for the soleus and the tibialis anterior muscles. It is suggested that the difference between the sarcomere number of the two muscles may result in the difference between the shape of curves of these two muscles. 6. Active torque-angle curves were established for the two muscles in situ. The shapes of the curves for soleus and tibialis anterior are similar in spite of the different mechanical conditions of the two muscles. This fact helps to explain why the two muscles, despite their similar articular range, had very different sarcomere number.", "PMID": 957266}
{"id": "pubmed23n0032_2632", "title": "The oxidation of glucose, ketone bodies and acetate by the brain of normal and ketonaemic sheep.", "content": "1. The utilization and oxidation of glucose, acetate and ketone bodies by the brain of sheep has been determined from measurements of arteriovenous (A-V) differences and cerebral blood flow, as well as by infusing 14C-labelled metabolites. 2. The A-V difference for glucose was generally more than one sixth, on a molar basis, that of oxygen. 3. The mean rate of glucose utilization by the brain of conscious sheep (0-508 +/- 0-063 mumole/g per minute) was maintained even when the capillary glucose concentration was below 1-4 mM. 4. The amount of 14CO2 produced from [U-14C]glucose by the brain was consistent with glucose being the only energy source for the brain, even during hypoglycaemia and hyperketonaemia. 5. There was no appreciable production of lactate or pyruvate by the brain. 6. There was no significant A-V difference for acetate across the brain in normal or undernourished pregnant sheep. The small A-V differences that were measured show that less than 5% of the CO2 produced could be derived from acetate, a conclusion that is supported by experiments using [U-14C]acetate. 7. No significant A-V difference was detectable across the brain for 3-hydroxybutyrate or acetoacetate in normal fed, pregnant ketonaemic or even anaesthetized sheep infused with acetoacetate. Experiments in which [U-14C]-D(-)-3-hydroxybutyrate was infused also showed that less than 5% of CO2 was derived from ketone bodies. 8. In anaesthetized sheep infused with acetoacetate, measurements were made simultaneously across brain, heart and skeletal muscle. In contrast to the non-significant uptake of ketone bodies by the brain, uptake by heart and skeletal muscle was sufficient to account for nearly 60% of their oxygen consumption. 9. Experiments using [14C]hydroxybutyrate confirmed that during infusion of acetoacetate most of the CO2 produced by the heart, but not by the brain, was derived from ketone bodies. 10. In anaesthetized sheep ketone bodies penetrate only slowly into cerebrospinal fluid. 11. It is proposed that mechanisms for the utilization of ketones by the sheep brain have not evolved because glucose utilization by the brain is a smaller fraction of whole body glucose utilization than in man and rats.", "contents": "The oxidation of glucose, ketone bodies and acetate by the brain of normal and ketonaemic sheep. 1. The utilization and oxidation of glucose, acetate and ketone bodies by the brain of sheep has been determined from measurements of arteriovenous (A-V) differences and cerebral blood flow, as well as by infusing 14C-labelled metabolites. 2. The A-V difference for glucose was generally more than one sixth, on a molar basis, that of oxygen. 3. The mean rate of glucose utilization by the brain of conscious sheep (0-508 +/- 0-063 mumole/g per minute) was maintained even when the capillary glucose concentration was below 1-4 mM. 4. The amount of 14CO2 produced from [U-14C]glucose by the brain was consistent with glucose being the only energy source for the brain, even during hypoglycaemia and hyperketonaemia. 5. There was no appreciable production of lactate or pyruvate by the brain. 6. There was no significant A-V difference for acetate across the brain in normal or undernourished pregnant sheep. The small A-V differences that were measured show that less than 5% of the CO2 produced could be derived from acetate, a conclusion that is supported by experiments using [U-14C]acetate. 7. No significant A-V difference was detectable across the brain for 3-hydroxybutyrate or acetoacetate in normal fed, pregnant ketonaemic or even anaesthetized sheep infused with acetoacetate. Experiments in which [U-14C]-D(-)-3-hydroxybutyrate was infused also showed that less than 5% of CO2 was derived from ketone bodies. 8. In anaesthetized sheep infused with acetoacetate, measurements were made simultaneously across brain, heart and skeletal muscle. In contrast to the non-significant uptake of ketone bodies by the brain, uptake by heart and skeletal muscle was sufficient to account for nearly 60% of their oxygen consumption. 9. Experiments using [14C]hydroxybutyrate confirmed that during infusion of acetoacetate most of the CO2 produced by the heart, but not by the brain, was derived from ketone bodies. 10. In anaesthetized sheep ketone bodies penetrate only slowly into cerebrospinal fluid. 11. It is proposed that mechanisms for the utilization of ketones by the sheep brain have not evolved because glucose utilization by the brain is a smaller fraction of whole body glucose utilization than in man and rats.", "PMID": 957265}
{"id": "pubmed23n0032_2633", "title": "Horseradish peroxidase localization of masticatory muscle motoneurons in cat.", "content": "Horseradish peroxidase has been injected into individual masticatory muscles in young and adult cats in order to determine the topography of the corresponding groups of motoneurons in the motor nucleus of the Vth nerve. The results obtained show a clear dorsoventral somatotopic distribution; the superior muscles have their motoneurons located dorsally in the nucleus and the inferior muscles ventrally; the two main jaw closers, temporalis and masseter, are represented in the dorsal and central parts of the nucleus; located more ventrally are the motoneurons for the pterygoideus medialis and lateralis, the jaw closers and abductor muscles; finally motoneurons for the jaw openers, and the anterior belly of the digastricus and mylohyoideus, occupy the ventromedial part of the nucleus. All muscles have been found to be represented along the entire length of the nucleus, with the same dorsoventral layering.", "contents": "Horseradish peroxidase localization of masticatory muscle motoneurons in cat. Horseradish peroxidase has been injected into individual masticatory muscles in young and adult cats in order to determine the topography of the corresponding groups of motoneurons in the motor nucleus of the Vth nerve. The results obtained show a clear dorsoventral somatotopic distribution; the superior muscles have their motoneurons located dorsally in the nucleus and the inferior muscles ventrally; the two main jaw closers, temporalis and masseter, are represented in the dorsal and central parts of the nucleus; located more ventrally are the motoneurons for the pterygoideus medialis and lateralis, the jaw closers and abductor muscles; finally motoneurons for the jaw openers, and the anterior belly of the digastricus and mylohyoideus, occupy the ventromedial part of the nucleus. All muscles have been found to be represented along the entire length of the nucleus, with the same dorsoventral layering.", "PMID": 957267}
{"id": "pubmed23n0032_2634", "title": "[Technic of long term neuron unit-recording on the free animal].", "content": "Technique of long-term recording from single neurons in brain of freely moving animals with floating mobile microelectrodes.", "contents": "[Technic of long term neuron unit-recording on the free animal]. Technique of long-term recording from single neurons in brain of freely moving animals with floating mobile microelectrodes.", "PMID": 957268}
{"id": "pubmed23n0032_2635", "title": "A cognitive model of musical sight-reading.", "content": "The author, a professional flutist and psychologist, interviewed four pianists noted for their sight-reading abilities. The results of the interviews are considered from several points of view. Sight-reading is analyzed as a problem in pattern recognition: a movement from a sonata by Handel is used to illustrate the principle of scanning for familiar patterns. The close relationship between musical sight-reading and the reading of conventional texts is also suggested. Finally, drawing on the findings of other studies, a congnitive model of musical sight-reading is proposed. The schematic model of interlocking information-processing systems explains the differences between skilled and unskilled sight-readers: it also explains why some experienced, professional musicians are poor sight-readers. Verification of the model is provided in an additional section in which conversations with unskilled sight-readers are reported.", "contents": "A cognitive model of musical sight-reading. The author, a professional flutist and psychologist, interviewed four pianists noted for their sight-reading abilities. The results of the interviews are considered from several points of view. Sight-reading is analyzed as a problem in pattern recognition: a movement from a sonata by Handel is used to illustrate the principle of scanning for familiar patterns. The close relationship between musical sight-reading and the reading of conventional texts is also suggested. Finally, drawing on the findings of other studies, a congnitive model of musical sight-reading is proposed. The schematic model of interlocking information-processing systems explains the differences between skilled and unskilled sight-readers: it also explains why some experienced, professional musicians are poor sight-readers. Verification of the model is provided in an additional section in which conversations with unskilled sight-readers are reported.", "PMID": 957269}
{"id": "pubmed23n0032_2636", "title": "[The development of arterial thrombi].", "content": "The factors involved in arterial thrombosis include the endothelial and subendothelial cells of the arterial wall, platelets and other blood cells, plasma factors and blood flow. An arterial thrombus arises from an interaction between platelets and the subendothelium (basement membrane and microfibrils) which has been shown by recent experiments to have a thrombogenic propensity. The particular structure of the subendothelium and its glycoprotein membrane receptors for platelets is implicated on thrombogenesis which requires the intervention of von Willebrand's factor; the well-known role of collagen is also important. An arterial thrombosis may evolve into a mural thrombus or may spread or form an embolus.", "contents": "[The development of arterial thrombi]. The factors involved in arterial thrombosis include the endothelial and subendothelial cells of the arterial wall, platelets and other blood cells, plasma factors and blood flow. An arterial thrombus arises from an interaction between platelets and the subendothelium (basement membrane and microfibrils) which has been shown by recent experiments to have a thrombogenic propensity. The particular structure of the subendothelium and its glycoprotein membrane receptors for platelets is implicated on thrombogenesis which requires the intervention of von Willebrand's factor; the well-known role of collagen is also important. An arterial thrombosis may evolve into a mural thrombus or may spread or form an embolus.", "PMID": 957275}
{"id": "pubmed23n0032_2637", "title": "[\"Cirrhotic's lund\". A new radiological entity? 182 CASES (AUTHOR'S TRANSL)].", "content": "The authors studied the chest X-rays of 182 patients with alcoholic cirrhosis. They eliminated the abnormal films of patients with severe clinical or biological changes. After double reading, they isolated ten cases in which chest X-ray revealed changes in the pulmonary interstitium. The question as to the nature of this interstitial pneumopathy is posed.", "contents": "[\"Cirrhotic's lund\". A new radiological entity? 182 CASES (AUTHOR'S TRANSL)]. The authors studied the chest X-rays of 182 patients with alcoholic cirrhosis. They eliminated the abnormal films of patients with severe clinical or biological changes. After double reading, they isolated ten cases in which chest X-ray revealed changes in the pulmonary interstitium. The question as to the nature of this interstitial pneumopathy is posed.", "PMID": 957282}
{"id": "pubmed23n0032_2638", "title": "[The treatment of hemoptysis by embolization of the systemic arteries (author's transl)].", "content": "The authors present their still limited experience of embolization of systemic arteries as the treatment of hemoptysis. Their cases illustrate the indications, contra-indications, causes of failure and effectiveness of this therapeutic method in pneumology. They had to refuse who patients: in the first, the pathological bronchial artery arose from a common trunk with the intercostal which gave rise to an anterior spinal artery. The second patient showed signs of medullary involvement on injection of a pathological right intercostal artery, which in their opinion is also a contra-indication of embolization, although no anterior spinal artery was demonstrated on the arteriogrammes. Six patients were treated by embolization. In the case of one patient, treatment was a failure owing to incomplete embolization. A main esophageal pedicle supplied a high flow bronchial artery anastomosis. These important collateral esophageal vessels compromize the effectiveness of bronchial embolization.", "contents": "[The treatment of hemoptysis by embolization of the systemic arteries (author's transl)]. The authors present their still limited experience of embolization of systemic arteries as the treatment of hemoptysis. Their cases illustrate the indications, contra-indications, causes of failure and effectiveness of this therapeutic method in pneumology. They had to refuse who patients: in the first, the pathological bronchial artery arose from a common trunk with the intercostal which gave rise to an anterior spinal artery. The second patient showed signs of medullary involvement on injection of a pathological right intercostal artery, which in their opinion is also a contra-indication of embolization, although no anterior spinal artery was demonstrated on the arteriogrammes. Six patients were treated by embolization. In the case of one patient, treatment was a failure owing to incomplete embolization. A main esophageal pedicle supplied a high flow bronchial artery anastomosis. These important collateral esophageal vessels compromize the effectiveness of bronchial embolization.", "PMID": 957284}
{"id": "pubmed23n0032_2639", "title": "[Arterial radio-anatomy of the extrahepatic biliary system (author's transl)].", "content": "The uni-arterial vascularisation of the lateral bilary duct makes it impossible to systematise the arteries which supply the common bile duct. Only the cystic artery is seen at coelio-mesenteric catheterization. The techniques essential for visualisation of the cystic artery are discussed, on the basis of one hundred coelio-mesenteric arteriograms: frequency of demonstration, origin, course, termination, calibre and length. The frequent finding of opacification of the wall of the gall bladder under normal conditions is stressed.", "contents": "[Arterial radio-anatomy of the extrahepatic biliary system (author's transl)]. The uni-arterial vascularisation of the lateral bilary duct makes it impossible to systematise the arteries which supply the common bile duct. Only the cystic artery is seen at coelio-mesenteric catheterization. The techniques essential for visualisation of the cystic artery are discussed, on the basis of one hundred coelio-mesenteric arteriograms: frequency of demonstration, origin, course, termination, calibre and length. The frequent finding of opacification of the wall of the gall bladder under normal conditions is stressed.", "PMID": 957285}
{"id": "pubmed23n0032_2640", "title": "[Radiological exploration of pulmonary systemic hypervascularization. Study of our results concerning 83 cases. Confrontation of the angiographic results with the existence of hemoptysis (author's transl)].", "content": "The authors present the results of selective bronchial and parietal arteriography of pulmonary systemic hypervascularization, in 83 cases. They emphasize the advantages of previous pulmonary venous angiography and overall aortography for the success of selective injections. Failures are evaluated at 6 to 7%. On occasions, a medullary branch was opacified. No medullary accidents were noted. The angiographic appearances of the lesions encountered are described. A comparative study of the angiographic results of non-tumoral acquired lesions was carried out in those patients with hemoptysis and in those who were free from them.", "contents": "[Radiological exploration of pulmonary systemic hypervascularization. Study of our results concerning 83 cases. Confrontation of the angiographic results with the existence of hemoptysis (author's transl)]. The authors present the results of selective bronchial and parietal arteriography of pulmonary systemic hypervascularization, in 83 cases. They emphasize the advantages of previous pulmonary venous angiography and overall aortography for the success of selective injections. Failures are evaluated at 6 to 7%. On occasions, a medullary branch was opacified. No medullary accidents were noted. The angiographic appearances of the lesions encountered are described. A comparative study of the angiographic results of non-tumoral acquired lesions was carried out in those patients with hemoptysis and in those who were free from them.", "PMID": 957283}
{"id": "pubmed23n0032_2641", "title": "[Varicoid carcinoma of oesophagus (author's transl)].", "content": "Two cases of diffuse squamous cell carcinoma of the oesophagus simulating the roentgenographic appearance of oesophageal varices are presented. Careful fluoroscopic examination may permit accurate diagnosis; however if the lesions are equivocally demonstrated by X-ray examination, oesophagoscopy confirms the diagnosis of carcinoma. R94.", "contents": "[Varicoid carcinoma of oesophagus (author's transl)]. Two cases of diffuse squamous cell carcinoma of the oesophagus simulating the roentgenographic appearance of oesophageal varices are presented. Careful fluoroscopic examination may permit accurate diagnosis; however if the lesions are equivocally demonstrated by X-ray examination, oesophagoscopy confirms the diagnosis of carcinoma. R94.", "PMID": 957286}
{"id": "pubmed23n0032_2642", "title": "[A rare hemopathy: urographic diagnosis (author's transl)].", "content": "A case of papillary necrosis was discovered by chance when doing a systematic urography  on a black woman suffering from subacute pains in the abdomen. This radiological information leads us to the finding of two hemoglobinopathies associated with a heterozygous shate: drepanocytemia and hemoglobin C disease. Clinically this list, nearly asymptomatic, is closed up by distinctive osseous lesions, the pains in the abdomen were due to a biliary lithiasis.", "contents": "[A rare hemopathy: urographic diagnosis (author's transl)]. A case of papillary necrosis was discovered by chance when doing a systematic urography  on a black woman suffering from subacute pains in the abdomen. This radiological information leads us to the finding of two hemoglobinopathies associated with a heterozygous shate: drepanocytemia and hemoglobin C disease. Clinically this list, nearly asymptomatic, is closed up by distinctive osseous lesions, the pains in the abdomen were due to a biliary lithiasis.", "PMID": 957288}
{"id": "pubmed23n0032_2643", "title": "[A case of ascaridiosis of the common bile duct].", "content": "The authors present a case of ascariasis of the common bile duct in a 45 year old Portugese woman. Perfusion cholangiography showed a lacunar ribbon shaped image of the common bile duct and radiolucent gall-stones. The diagnosis of intracholedochal ascariasis was made and the patient operated: there was in fact an ascaris which was extracted by choledochotomy. Cholecystectomy. Straightforward aftermath.", "contents": "[A case of ascaridiosis of the common bile duct]. The authors present a case of ascariasis of the common bile duct in a 45 year old Portugese woman. Perfusion cholangiography showed a lacunar ribbon shaped image of the common bile duct and radiolucent gall-stones. The diagnosis of intracholedochal ascariasis was made and the patient operated: there was in fact an ascaris which was extracted by choledochotomy. Cholecystectomy. Straightforward aftermath.", "PMID": 957289}
{"id": "pubmed23n0032_2644", "title": "[Secreting paragangliomas of the organ of Zuckerkandl. A report on two cases (author's transl)].", "content": "The authors describe two cases of secreting paragangliomas of the organ of Zuckerkandl, beinging the number of known cases to 78. They underline the interest of arteriographic and phlebographic explorations which define more accurately the origin and spread of these tumours.", "contents": "[Secreting paragangliomas of the organ of Zuckerkandl. A report on two cases (author's transl)]. The authors describe two cases of secreting paragangliomas of the organ of Zuckerkandl, beinging the number of known cases to 78. They underline the interest of arteriographic and phlebographic explorations which define more accurately the origin and spread of these tumours.", "PMID": 957287}
{"id": "pubmed23n0032_2645", "title": "[A simplified radiological technique for measurement of the angle of anteversion of the femur and of the cervico-diaphyseal angle (author's transl)].", "content": "Description of a technique for measurement of the angles of the upper extremity of the femur, applicable to all patients, without mobilisation, and using a remote controlled table with two localised films in a frontal projection, and a simple construction using a ruler and protractor.", "contents": "[A simplified radiological technique for measurement of the angle of anteversion of the femur and of the cervico-diaphyseal angle (author's transl)]. Description of a technique for measurement of the angles of the upper extremity of the femur, applicable to all patients, without mobilisation, and using a remote controlled table with two localised films in a frontal projection, and a simple construction using a ruler and protractor.", "PMID": 957290}
{"id": "pubmed23n0032_2646", "title": "A study of bereavement in general practice.", "content": "Forty six bereaved relatives were assessed by a general practitioner four to eight weeks after the bereavement. In 36 (78.3 per cent) the immediate reaction to bereavement was one of numbness or stupefaction; in seven (15.2 per cent) emotional relief occurred; and in three cases (6.5 per cent) there was no obvious immediate reaction. The numbness reaction was limited in duration to a week or less in 31 of the 36 instances.At four to eight weeks after bereavement 29 (63.0 per cent) of the subjects continued to experience difficulty in coming to terms with their loss. Twenty subjects reported guilt feelings and a similar number expressed aggressive reactions. The bereaved subjects tended to increase their consumption of cigarettes and alcohol, while their appetite and weight tended to be reduced. Thirty six (78.3 per cent) of the subjects reported physical symptoms, notably headache, dizziness, generalised aches, and abdominal complaints.THE MOST PROMINENT PSYCHOLOGICAL FEATURES OF BEREAVEMENT WERE FOUND TO BE: preoccupation with thoughts of the deceased, idealisation of the lost person, depressive mood, and loneliness.The findings are discussed and reference made to the role of the family doctor in the management of bereavement reactions.", "contents": "A study of bereavement in general practice. Forty six bereaved relatives were assessed by a general practitioner four to eight weeks after the bereavement. In 36 (78.3 per cent) the immediate reaction to bereavement was one of numbness or stupefaction; in seven (15.2 per cent) emotional relief occurred; and in three cases (6.5 per cent) there was no obvious immediate reaction. The numbness reaction was limited in duration to a week or less in 31 of the 36 instances.At four to eight weeks after bereavement 29 (63.0 per cent) of the subjects continued to experience difficulty in coming to terms with their loss. Twenty subjects reported guilt feelings and a similar number expressed aggressive reactions. The bereaved subjects tended to increase their consumption of cigarettes and alcohol, while their appetite and weight tended to be reduced. Thirty six (78.3 per cent) of the subjects reported physical symptoms, notably headache, dizziness, generalised aches, and abdominal complaints.THE MOST PROMINENT PSYCHOLOGICAL FEATURES OF BEREAVEMENT WERE FOUND TO BE: preoccupation with thoughts of the deceased, idealisation of the lost person, depressive mood, and loneliness.The findings are discussed and reference made to the role of the family doctor in the management of bereavement reactions.", "PMID": 957295}
{"id": "pubmed23n0032_2647", "title": "[Techniques for opacification of the lumbar spinal venous plexuses (author's transl)].", "content": "No single technique exists for lumbar phlebography. Whilst catheterisation of the ilio-lumbar veins is always the first step, it should be routinely followed by injection of the lateral sacral veins whenever the leel L5-S1 is clinically suspicious. Finally, when only poor opacification of the longitudinal epidural plexuses is obtained by filateral injection of the ilio-lumbar or ilio-lumbar and sacral systems, catheterisation of the vein in L2 on the right or L3 on the left makes it possible in virtually all cases to obtain satisfactory filling of the lumbar plexuses. It may be considered at the present time that, from a technical standpoint, failures of lumbar phlebography by bilateral femoral catheterisation do not exceed 1% of cases.", "contents": "[Techniques for opacification of the lumbar spinal venous plexuses (author's transl)]. No single technique exists for lumbar phlebography. Whilst catheterisation of the ilio-lumbar veins is always the first step, it should be routinely followed by injection of the lateral sacral veins whenever the leel L5-S1 is clinically suspicious. Finally, when only poor opacification of the longitudinal epidural plexuses is obtained by filateral injection of the ilio-lumbar or ilio-lumbar and sacral systems, catheterisation of the vein in L2 on the right or L3 on the left makes it possible in virtually all cases to obtain satisfactory filling of the lumbar plexuses. It may be considered at the present time that, from a technical standpoint, failures of lumbar phlebography by bilateral femoral catheterisation do not exceed 1% of cases.", "PMID": 957292}
{"id": "pubmed23n0032_2648", "title": "[The treatment of herpes zoster by ultraviolet actinotherapy. Study of 200 cases (author's transl)].", "content": "The authors analysed the cases of 200 patients treated for early herpes zoster by ultraviolet actinotherapy. This simple treatment should ideally be applied: - to clean skin, without dye nor ointment; - over the entire root area, and not only to the vesicles; - obtaining from the outset frank erythema, uniform, and without a healthy skin interval. Under these conditions, 95% of successful results were obtained in 3 to 8 days. The occasional failures appear to be related either to faulty technique or to a deep-seated localisation beyond the fied of action of the ultraviolet rays.", "contents": "[The treatment of herpes zoster by ultraviolet actinotherapy. Study of 200 cases (author's transl)]. The authors analysed the cases of 200 patients treated for early herpes zoster by ultraviolet actinotherapy. This simple treatment should ideally be applied: - to clean skin, without dye nor ointment; - over the entire root area, and not only to the vesicles; - obtaining from the outset frank erythema, uniform, and without a healthy skin interval. Under these conditions, 95% of successful results were obtained in 3 to 8 days. The occasional failures appear to be related either to faulty technique or to a deep-seated localisation beyond the fied of action of the ultraviolet rays.", "PMID": 957291}
{"id": "pubmed23n0032_2649", "title": "Examination of the P wave in lead V1 of the electrocardiogram: its value in routine electrocardiography.", "content": "While reporting on the electrocardiograms recorded in this Department during the past four-and-a-half years, interest was aroused by the occasional appearance of a terminal negative component in the P wave in lead V(1). I tried to find out what significance, if any, it had. In the ensuing study a relationship between such a negative component-expressed as a terminal negative force (-ve Ptf V(1) for short)-and the presence of ischaemic heart disease emerged. Such a negative Ptf V(1) is not an in-fallible marker of ischaemic heart disease nor does it necessarily persist in the repeat electrocardiogram of any given patient. It is, however, suggested that attention be routinely paid to the morphology of the P wave in lead V(1) and that when a negative terminal component is present in this wave follow-up study of this feature in serial electrocardiograms may help in the assessment of prognosis in any given patient.", "contents": "Examination of the P wave in lead V1 of the electrocardiogram: its value in routine electrocardiography. While reporting on the electrocardiograms recorded in this Department during the past four-and-a-half years, interest was aroused by the occasional appearance of a terminal negative component in the P wave in lead V(1). I tried to find out what significance, if any, it had. In the ensuing study a relationship between such a negative component-expressed as a terminal negative force (-ve Ptf V(1) for short)-and the presence of ischaemic heart disease emerged. Such a negative Ptf V(1) is not an in-fallible marker of ischaemic heart disease nor does it necessarily persist in the repeat electrocardiogram of any given patient. It is, however, suggested that attention be routinely paid to the morphology of the P wave in lead V(1) and that when a negative terminal component is present in this wave follow-up study of this feature in serial electrocardiograms may help in the assessment of prognosis in any given patient.", "PMID": 957296}
{"id": "pubmed23n0032_2650", "title": "The treatment of pain trigger areas in migraine.", "content": "In many patients with migraine, several localised areas usually on the scalp or neck can be identified. The local injection of lignocaine one per cent and adrenaline 1/200,000 often leads to considerable relief of the symptoms of migraine including visual phenomena. The common sites of the trigger areas are shown and several uncontrolled case reports of the effect of the treatment are described.", "contents": "The treatment of pain trigger areas in migraine. In many patients with migraine, several localised areas usually on the scalp or neck can be identified. The local injection of lignocaine one per cent and adrenaline 1/200,000 often leads to considerable relief of the symptoms of migraine including visual phenomena. The common sites of the trigger areas are shown and several uncontrolled case reports of the effect of the treatment are described.", "PMID": 957299}
{"id": "pubmed23n0032_2651", "title": "A survey of the effects of a drug promotion campaign.", "content": "A postal survey which tried to test the impact of a drug-promotion campaign is described. One hundred and twenty randomly-selected general practitioners were circulated and a 74.8 per cent response was obtained. Of the responders, 92 per cent were aware of the existence of the preparation, and of these responders 69 per cent were unaware either of the pharmacologically approved name of the drug, or the fact that its pharmacologically active constituent was already available under another brand name.", "contents": "A survey of the effects of a drug promotion campaign. A postal survey which tried to test the impact of a drug-promotion campaign is described. One hundred and twenty randomly-selected general practitioners were circulated and a 74.8 per cent response was obtained. Of the responders, 92 per cent were aware of the existence of the preparation, and of these responders 69 per cent were unaware either of the pharmacologically approved name of the drug, or the fact that its pharmacologically active constituent was already available under another brand name.", "PMID": 957301}
{"id": "pubmed23n0032_2652", "title": "A research project as a means for continuing education.", "content": "A project designed to encourage general practitioners to provide care in the earliest phases of coronary heart disease is reported from South Africa. A total of 129 general practitioners were actively involved and over 3,000 practitioners showed interest and attended at least one meeting.", "contents": "A research project as a means for continuing education. A project designed to encourage general practitioners to provide care in the earliest phases of coronary heart disease is reported from South Africa. A total of 129 general practitioners were actively involved and over 3,000 practitioners showed interest and attended at least one meeting.", "PMID": 957302}
{"id": "pubmed23n0032_2653", "title": "Symptoms perceived and recorded by patients.", "content": "Health diaries were kept by 198 randomly-chosen women between the ages of 20 and 44. Symptoms were recorded on one day in three and on 57 per cent of symptom-days self-medication occurred. Even a minor shift from self-care to doctor care could make intolerable demands on the general-practitioner service in this country.Only a minority of symptoms (one in 37) are taken to the doctor and patients are highly selective in deciding which symptoms are appropriate for medical care. The perception of symptoms and the response of seeking medical advice are both significantly related to anxiety as measured by a personality questionnaire.", "contents": "Symptoms perceived and recorded by patients. Health diaries were kept by 198 randomly-chosen women between the ages of 20 and 44. Symptoms were recorded on one day in three and on 57 per cent of symptom-days self-medication occurred. Even a minor shift from self-care to doctor care could make intolerable demands on the general-practitioner service in this country.Only a minority of symptoms (one in 37) are taken to the doctor and patients are highly selective in deciding which symptoms are appropriate for medical care. The perception of symptoms and the response of seeking medical advice are both significantly related to anxiety as measured by a personality questionnaire.", "PMID": 957305}
{"id": "pubmed23n0032_2654", "title": "Non-attenders in general practice.", "content": "Non-attenders for five years or longer (77 men and 27 women; i.e. 1.9 per cent of the practice) in a general practice of 5,750 patients were identified and paired with controls in the same practice. A survey by questionnaire was carried out.The conclusions were:(1) A higher proportion of non-attenders are self-employed,(2) A lower proportion of them had retired than their attending counterparts,(3) They are spread over the whole age-range, but with a higher concentration in the ages 35 and over,(4) They are evenly spread over the social classes,(5) They seldom seek other sources of medical advice or treatment,(6) They are significantly slimmer than their fellows,(7) Their non-attendance does not, on the whole, cloak serious but remediable illness.", "contents": "Non-attenders in general practice. Non-attenders for five years or longer (77 men and 27 women; i.e. 1.9 per cent of the practice) in a general practice of 5,750 patients were identified and paired with controls in the same practice. A survey by questionnaire was carried out.The conclusions were:(1) A higher proportion of non-attenders are self-employed,(2) A lower proportion of them had retired than their attending counterparts,(3) They are spread over the whole age-range, but with a higher concentration in the ages 35 and over,(4) They are evenly spread over the social classes,(5) They seldom seek other sources of medical advice or treatment,(6) They are significantly slimmer than their fellows,(7) Their non-attendance does not, on the whole, cloak serious but remediable illness.", "PMID": 957306}
{"id": "pubmed23n0032_2655", "title": "Self-medication in a small community.", "content": "(1) Simultaneous recording of doctor/patient contacts and chemists' sales were made during a period of two weeks in a small self-contained community.(2) The conclusion of previous studies, using different methods, that non-prescribed medicines are taken twice as often as those prescribed by doctors, is confirmed.(3) The possibility that self-medication may conceal the onset of serious disease is discussed.", "contents": "Self-medication in a small community. (1) Simultaneous recording of doctor/patient contacts and chemists' sales were made during a period of two weeks in a small self-contained community.(2) The conclusion of previous studies, using different methods, that non-prescribed medicines are taken twice as often as those prescribed by doctors, is confirmed.(3) The possibility that self-medication may conceal the onset of serious disease is discussed.", "PMID": 957307}
{"id": "pubmed23n0032_2656", "title": "Measuring learning by trainees in general practice.", "content": "Twenty simulated consultations with patients having a respiratory illness were carried out by 20 trainees at the start and finish of a training year in general practice, using the same method as used in a previous study of principals in general practice.During the course of the year, the trainees as a group closely approached the behaviour-in the defined terms of the study-of principals as a group. The trend was more marked for doctors on a three-year training programme than for those on a one-year programme.In 11 cases direct comparison between trainee and trainer was possible. It was difficult to identify changes in behaviour as being due to either group influences or individual trainer influences, but it appeared that atypical trainers do not necessarily produce atypical trainees and typical trainers do not prevent the development of individuality in trainees.The technique of simulated consultation may assist the difficult task of evaluating training for general practice.", "contents": "Measuring learning by trainees in general practice. Twenty simulated consultations with patients having a respiratory illness were carried out by 20 trainees at the start and finish of a training year in general practice, using the same method as used in a previous study of principals in general practice.During the course of the year, the trainees as a group closely approached the behaviour-in the defined terms of the study-of principals as a group. The trend was more marked for doctors on a three-year training programme than for those on a one-year programme.In 11 cases direct comparison between trainee and trainer was possible. It was difficult to identify changes in behaviour as being due to either group influences or individual trainer influences, but it appeared that atypical trainers do not necessarily produce atypical trainees and typical trainers do not prevent the development of individuality in trainees.The technique of simulated consultation may assist the difficult task of evaluating training for general practice.", "PMID": 957308}
{"id": "pubmed23n0032_2657", "title": "Hospital admission rates and the primary health team.", "content": "The use of hospital beds was studied for a period of one year in a practice under the care of a closely co-ordinated team composed of the family physician, the family nurse, and a medical social worker. Admission rates and mean duration of stay in hospital are analysed and discussed in relation to selected socio-demographic variables.The results showed that the study population used only half as many hospital beds when compared with national rates in Israel. We consider that this was achieved by the provision of planned co-ordinated comprehensive medical nursing and social services for patients suffering from long-term illness. Such a team is able to provide a high quality medical care and can significanlty reduce the use of in-patient hospital services.", "contents": "Hospital admission rates and the primary health team. The use of hospital beds was studied for a period of one year in a practice under the care of a closely co-ordinated team composed of the family physician, the family nurse, and a medical social worker. Admission rates and mean duration of stay in hospital are analysed and discussed in relation to selected socio-demographic variables.The results showed that the study population used only half as many hospital beds when compared with national rates in Israel. We consider that this was achieved by the provision of planned co-ordinated comprehensive medical nursing and social services for patients suffering from long-term illness. Such a team is able to provide a high quality medical care and can significanlty reduce the use of in-patient hospital services.", "PMID": 957309}
{"id": "pubmed23n0032_2658", "title": "Cold sores-an epidemiological survey.", "content": "A survey was carried out in a general practice in North Wales to determine the incidence, frequency, duration, and severity of cold sores. Of the 1,855 adults interviewed, 46 per cent gave a definite history of having had a cold sore at some time while 28 per cent continued to have one or more a year. More than half of the latter considered their sores of moderate or severe inconvenience.", "contents": "Cold sores-an epidemiological survey. A survey was carried out in a general practice in North Wales to determine the incidence, frequency, duration, and severity of cold sores. Of the 1,855 adults interviewed, 46 per cent gave a definite history of having had a cold sore at some time while 28 per cent continued to have one or more a year. More than half of the latter considered their sores of moderate or severe inconvenience.", "PMID": 957310}
{"id": "pubmed23n0032_2659", "title": "Second opinion. A study of medical referrals in a seminar for general practitioners at the Tavistock Clinic, London.", "content": "An experienced seminar made a special study of the psychological aspects of referrals for second opinions, investigations, or hospital admission. The cases were a random selection, incidentally forcing the seminar to examine some of the poor medical practice that is rarely published and to recognise the disturbing force of the doctor-illness relationship, as distinct from the doctor-patient relationship. General practitioners use referrals in many ways and select consultants for quite unexpected qualities, often to preserve the relationship with their patients, and contrary to many superficial impressions otherwise. Referrals are part of the continuous flux of patients in and out of a doctor's life and this movement is important to his sense of identity and to his defences against anxiety.", "contents": "Second opinion. A study of medical referrals in a seminar for general practitioners at the Tavistock Clinic, London. An experienced seminar made a special study of the psychological aspects of referrals for second opinions, investigations, or hospital admission. The cases were a random selection, incidentally forcing the seminar to examine some of the poor medical practice that is rarely published and to recognise the disturbing force of the doctor-illness relationship, as distinct from the doctor-patient relationship. General practitioners use referrals in many ways and select consultants for quite unexpected qualities, often to preserve the relationship with their patients, and contrary to many superficial impressions otherwise. Referrals are part of the continuous flux of patients in and out of a doctor's life and this movement is important to his sense of identity and to his defences against anxiety.", "PMID": 957313}
{"id": "pubmed23n0032_2660", "title": "First encounters.", "content": "Some conclusions are reported from a series of seminars involving several experienced general practitioners over a period of three years. The aim was to examine first consultations in general practice.We found that these were often handled superficially and that both doctors and patients seemed hesitant in their new relationship. We believe that the loss of a trusted family doctor can create a bereavement reaction in patients, especially where the relationship has been long and when the doctor leaves or dies suddenly.We are investigating the possibility that the death rate is increased among patients who have recently lost their general practitioner suddenly.", "contents": "First encounters. Some conclusions are reported from a series of seminars involving several experienced general practitioners over a period of three years. The aim was to examine first consultations in general practice.We found that these were often handled superficially and that both doctors and patients seemed hesitant in their new relationship. We believe that the loss of a trusted family doctor can create a bereavement reaction in patients, especially where the relationship has been long and when the doctor leaves or dies suddenly.We are investigating the possibility that the death rate is increased among patients who have recently lost their general practitioner suddenly.", "PMID": 957314}
{"id": "pubmed23n0032_2661", "title": "Breast feeding--a year's experience of one group practice.", "content": "Dissatisfaction at the lack of support and encouragement in the immediate post-partum period of mothers in hospital wishing to breast feed is expressed in their own words. Analysis indicates that mothers in the upper social classes are more likely to breast feed and that those who manage to sustain breast feeding for one week are likely to continue this method of feeding for at least six weeks and possibly for much longer. There is also evidence indicating that women who are encouraged during pregnancy to breast feed have a high success rate. Those responsible for supervising infant feeding need to be better educated in all its aspects.", "contents": "Breast feeding--a year's experience of one group practice. Dissatisfaction at the lack of support and encouragement in the immediate post-partum period of mothers in hospital wishing to breast feed is expressed in their own words. Analysis indicates that mothers in the upper social classes are more likely to breast feed and that those who manage to sustain breast feeding for one week are likely to continue this method of feeding for at least six weeks and possibly for much longer. There is also evidence indicating that women who are encouraged during pregnancy to breast feed have a high success rate. Those responsible for supervising infant feeding need to be better educated in all its aspects.", "PMID": 957315}
{"id": "pubmed23n0032_2662", "title": "Treating sexual dysfunction in general practice.", "content": "A method of training general practitioners in the treatment of sexual dysfunction is described, using fortnightly seminars at which the doctors discussed the continuing care of their patients.Doctors took on patients presenting in their practices and treated couples together, where possible, using a mixture of insight-directed and behavioural techniques similar to those used by Masters and Johnson (1970). Interviews were reported back to the group which gave advice and support. The doctors, all beginners in this type of work, were able to help substantially 72 per cent of 47 couples treated.", "contents": "Treating sexual dysfunction in general practice. A method of training general practitioners in the treatment of sexual dysfunction is described, using fortnightly seminars at which the doctors discussed the continuing care of their patients.Doctors took on patients presenting in their practices and treated couples together, where possible, using a mixture of insight-directed and behavioural techniques similar to those used by Masters and Johnson (1970). Interviews were reported back to the group which gave advice and support. The doctors, all beginners in this type of work, were able to help substantially 72 per cent of 47 couples treated.", "PMID": 957316}
{"id": "pubmed23n0032_2663", "title": "A retrospective survey of over 1,000 patients on oral contraceptives in a group practice.", "content": "The side-effects encountered by 1,090 patients taking the oral contraceptive pill were reviewed. The main side-effects were migraine, headaches, weight gain, depression, and irregular bleeding. Reasons for changing the Pill are discussed, and recommendations are suggested for a way of monitoring patients taking the Pill. Analysis of the results show that 50 per cent of patients are happy with their first Pill, and that the 30 microgram pill produces fewer side-effects.", "contents": "A retrospective survey of over 1,000 patients on oral contraceptives in a group practice. The side-effects encountered by 1,090 patients taking the oral contraceptive pill were reviewed. The main side-effects were migraine, headaches, weight gain, depression, and irregular bleeding. Reasons for changing the Pill are discussed, and recommendations are suggested for a way of monitoring patients taking the Pill. Analysis of the results show that 50 per cent of patients are happy with their first Pill, and that the 30 microgram pill produces fewer side-effects.", "PMID": 957317}
{"id": "pubmed23n0032_2664", "title": "The first three months of free contraception in a market-town group practice.", "content": "Contraceptive services became free to patients of general practitioners in the British National Health Service on 1 July 1975. We report the numbers of patients advised in a practice of 6,612 patients during the first three-month period and found that in a sample of 211 women, just over half had received medical advice. The financial implications are discussed.", "contents": "The first three months of free contraception in a market-town group practice. Contraceptive services became free to patients of general practitioners in the British National Health Service on 1 July 1975. We report the numbers of patients advised in a practice of 6,612 patients during the first three-month period and found that in a sample of 211 women, just over half had received medical advice. The financial implications are discussed.", "PMID": 957318}
{"id": "pubmed23n0032_2665", "title": "Hormonal changes associated with experimentally produced cystic ovaries in the cow.", "content": "Cystic ovaries were experimentally produced by i.m. injection of 5 mg oestradiol valerate on Day 16 of the oestrous cycle or by s.c. injection of 100 ml antiserum to bovine LH before the onset of oestrus. The length of the cycle after both treatments was altered. The cysts formed after oestradiol injection were relatively small (2-5-3 cm diameter) and thin-walled, and were accompanied by relatively low plasma oestrone and high oestradiol levels; those after LH antiserum treatment were large (5-0-6-0 cm) and firm and associated with relatively low oestradiol and high oestrone levels. The results of the hormone assays after treatment support the theory that cystic ovaries could result from premature LH release or an insufficiency of LH at the time of ovulation.", "contents": "Hormonal changes associated with experimentally produced cystic ovaries in the cow. Cystic ovaries were experimentally produced by i.m. injection of 5 mg oestradiol valerate on Day 16 of the oestrous cycle or by s.c. injection of 100 ml antiserum to bovine LH before the onset of oestrus. The length of the cycle after both treatments was altered. The cysts formed after oestradiol injection were relatively small (2-5-3 cm diameter) and thin-walled, and were accompanied by relatively low plasma oestrone and high oestradiol levels; those after LH antiserum treatment were large (5-0-6-0 cm) and firm and associated with relatively low oestradiol and high oestrone levels. The results of the hormone assays after treatment support the theory that cystic ovaries could result from premature LH release or an insufficiency of LH at the time of ovulation.", "PMID": 957319}
{"id": "pubmed23n0032_2666", "title": "The ultrastructure of the thecal cell of the teleost, Oryzias latipes, during ovulation in vitro.", "content": "Ultrastructural examination of the follicular envelope of the teleost, Oryzias latipes, during ovulation in vitro showed that the follicle cell layer became increasingly dissociated. Microfilaments, measuring 50-70 A, were sparse in the thecal cells at the start of incubation, but were organized into bands after 5 hr, and into bundles after 10 hr of incubation. It is suggested that these microfilaments are contractile and are involved in cell movement during ovulation.", "contents": "The ultrastructure of the thecal cell of the teleost, Oryzias latipes, during ovulation in vitro. Ultrastructural examination of the follicular envelope of the teleost, Oryzias latipes, during ovulation in vitro showed that the follicle cell layer became increasingly dissociated. Microfilaments, measuring 50-70 A, were sparse in the thecal cells at the start of incubation, but were organized into bands after 5 hr, and into bundles after 10 hr of incubation. It is suggested that these microfilaments are contractile and are involved in cell movement during ovulation.", "PMID": 957320}
{"id": "pubmed23n0032_2667", "title": "Effects of dietary caffeine on the testis of the domestic fowl, Gallus domesticus.", "content": "Roosters were fed 0-1% caffeine mixed by weight into a standard ration. With continued dietary caffeine administration, the average fertility of eggs collected for 2 weeks from untreated pullets inseminated with semen from the treated males at 0, 7 and 14 days after the start of treatment was 30-8, 33-5 and 3-3%, respectively. After 14 days of treatment fertility was significantly lower (P less than 0-001) than before (0 days) or 7 days after treatment. Semen output and sperm concentration were markedly reduced 17-21 days after treatment, and no semen could be collected from the roosters after they had received caffeine for 30 days. Removal of dietary caffeine resulted in resumption of semen production and a return of fertility to the control level. Testicular histology showed that spermatocyte divisions ceased and spermiogenesis was abnormal, although Leydig tissue and the response of the males to massage for semen collection was not affected. The effects on spermatogenesis and fertility were reversible after treatment for 30 days.", "contents": "Effects of dietary caffeine on the testis of the domestic fowl, Gallus domesticus. Roosters were fed 0-1% caffeine mixed by weight into a standard ration. With continued dietary caffeine administration, the average fertility of eggs collected for 2 weeks from untreated pullets inseminated with semen from the treated males at 0, 7 and 14 days after the start of treatment was 30-8, 33-5 and 3-3%, respectively. After 14 days of treatment fertility was significantly lower (P less than 0-001) than before (0 days) or 7 days after treatment. Semen output and sperm concentration were markedly reduced 17-21 days after treatment, and no semen could be collected from the roosters after they had received caffeine for 30 days. Removal of dietary caffeine resulted in resumption of semen production and a return of fertility to the control level. Testicular histology showed that spermatocyte divisions ceased and spermiogenesis was abnormal, although Leydig tissue and the response of the males to massage for semen collection was not affected. The effects on spermatogenesis and fertility were reversible after treatment for 30 days.", "PMID": 957321}
{"id": "pubmed23n0032_2668", "title": "Effects of androgens on sexual behaviour and somatic variables in the male golden hamster.", "content": "Comparisons were made of the sexual behaviour of sham-operated male hamsters and castrated males receiving testosterone, dihydrotestosterone or androstenedione (1-5 mg/week), or oil alone. Tests of short duration (10 min) were conducted at Week 3 (when the animals were sexually naive), Week 6 and Week 9. Sham-operated males showed marked increases in many elements of behaviour between Weeks 3 and 9, while castrated males receiving no androgen replacement showed marked decreases. Males receiving each of the three androgens showed marked increases in behaviour, but androstenedione-treated males showed less facilitation of sexual behaviour than controls. Dihydrotestosterone was as effective as testosterone. The three androgens were comparable in maintaining seminal vesicle weight after castration and in preventing the customary rise in pituitary and body weights. These data suggest that, unlike the situation in the rat, aromatization of androgens is unnecessary for the display of sexual behaviour in the hamster.", "contents": "Effects of androgens on sexual behaviour and somatic variables in the male golden hamster. Comparisons were made of the sexual behaviour of sham-operated male hamsters and castrated males receiving testosterone, dihydrotestosterone or androstenedione (1-5 mg/week), or oil alone. Tests of short duration (10 min) were conducted at Week 3 (when the animals were sexually naive), Week 6 and Week 9. Sham-operated males showed marked increases in many elements of behaviour between Weeks 3 and 9, while castrated males receiving no androgen replacement showed marked decreases. Males receiving each of the three androgens showed marked increases in behaviour, but androstenedione-treated males showed less facilitation of sexual behaviour than controls. Dihydrotestosterone was as effective as testosterone. The three androgens were comparable in maintaining seminal vesicle weight after castration and in preventing the customary rise in pituitary and body weights. These data suggest that, unlike the situation in the rat, aromatization of androgens is unnecessary for the display of sexual behaviour in the hamster.", "PMID": 957322}
{"id": "pubmed23n0032_2669", "title": "Mechanisms of action of the antifertility agents U11, 100A and U11,555A in the rat.", "content": "Mature female rats were given U11,555A and U11,100A on orally on Day 2 of pregnancy and the number of implantations counted on Day 9. Using doses which reduced the implantation number to approximately 40% that of the controls, it was shown that the reduced number was not due to an effect on egg transport or to a delay of implantation.", "contents": "Mechanisms of action of the antifertility agents U11, 100A and U11,555A in the rat. Mature female rats were given U11,555A and U11,100A on orally on Day 2 of pregnancy and the number of implantations counted on Day 9. Using doses which reduced the implantation number to approximately 40% that of the controls, it was shown that the reduced number was not due to an effect on egg transport or to a delay of implantation.", "PMID": 957323}
{"id": "pubmed23n0032_2670", "title": "The lipids of buffalo spermatozoa and seminal plasma.", "content": "Lipids were extracted from the spermatozoa and seminal plasma of buffalo and were analysed by column and thin-layer chromatography. Buffalo spermatozoa (10(9) cells) contained 1-15 mg total lipids, 0-285 mg neutral lipids, 0-396 mg glycolipids, 0-547 mg phospholipids and 0-015 mg gangliosides. The corresponding values for 1 ml seminal plasma were: 1-50, 0-439, 0-581, 0-594 and 0-010 mg. The sperm neutral lipids were comprised of 3-2% hydrocarbons, 4-5% cholesterol esters, 46-7% triglycerides, 17-5% 1,3-diglycerides, 3-1% 1,2-diglycerides, 17-6% cholesterol, 0-6% free fatty acids and 2-4% monoglycerides. The corresponding values for seminal plasma were: 4-2, 6-2, 33-0, 19-8, 4-2, 18-5, 3-8 and 4-7%. In the sperm phospholipids, there was 30-4% phosphatidyl choline; 19-4% phosphatidal choline; 10-8% phosphatidyl ethanolamine; 3-4% phosphatidal ethanolamine; 11-3% sphingomyelin; 1-5% phosphatidyl serine; 0-6% phosphatidyl inositol; 3-9% lysophosphatidyl choline; 4-4% lysophosphatidyl ethanolamine; 0-7% lysophosphatidyl serine; 5-5% diphosphatidyl glycerol and 0-3% phosphatidic acid. The corresponding values for seminal plasma were: 21-7, 17-3, 11-7, 4-1, 13-1, 2-8, 3-9, 5-6, 1-0, 7-4 and 0-5%. Comparative data for bull spermatozoa and seminal plasma were also obtained.", "contents": "The lipids of buffalo spermatozoa and seminal plasma. Lipids were extracted from the spermatozoa and seminal plasma of buffalo and were analysed by column and thin-layer chromatography. Buffalo spermatozoa (10(9) cells) contained 1-15 mg total lipids, 0-285 mg neutral lipids, 0-396 mg glycolipids, 0-547 mg phospholipids and 0-015 mg gangliosides. The corresponding values for 1 ml seminal plasma were: 1-50, 0-439, 0-581, 0-594 and 0-010 mg. The sperm neutral lipids were comprised of 3-2% hydrocarbons, 4-5% cholesterol esters, 46-7% triglycerides, 17-5% 1,3-diglycerides, 3-1% 1,2-diglycerides, 17-6% cholesterol, 0-6% free fatty acids and 2-4% monoglycerides. The corresponding values for seminal plasma were: 4-2, 6-2, 33-0, 19-8, 4-2, 18-5, 3-8 and 4-7%. In the sperm phospholipids, there was 30-4% phosphatidyl choline; 19-4% phosphatidal choline; 10-8% phosphatidyl ethanolamine; 3-4% phosphatidal ethanolamine; 11-3% sphingomyelin; 1-5% phosphatidyl serine; 0-6% phosphatidyl inositol; 3-9% lysophosphatidyl choline; 4-4% lysophosphatidyl ethanolamine; 0-7% lysophosphatidyl serine; 5-5% diphosphatidyl glycerol and 0-3% phosphatidic acid. The corresponding values for seminal plasma were: 21-7, 17-3, 11-7, 4-1, 13-1, 2-8, 3-9, 5-6, 1-0, 7-4 and 0-5%. Comparative data for bull spermatozoa and seminal plasma were also obtained.", "PMID": 957324}
{"id": "pubmed23n0032_2671", "title": "Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid.", "content": "Analysis of the fatty acids of total and neutral lipids, glycolipids, phospholipids and gangliosides of buffalo spermatozoa and seminal plasma showed that there were high levels of polyunsaturated acids. Neutral lipids were the richest in polyunsaturated acids (55% in spermatozoa and 61% in seminal plasma). The major saturated acid of all the principal classes was stearic acid and the major unsaturated acid was docosahexaenoic acid (22:6omega3) except in the neutral lipids in which it was arachidonic acid (20:4omega6). The major aldehyde was palmitaldehyde (16:0) in buffalo sperm lipids and docosanal (22:0) in seminal plasma. More than 50% of the total aldehydes was contributed by aldehydes with a chain length greater than 18 carbon atoms.", "contents": "Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid. Analysis of the fatty acids of total and neutral lipids, glycolipids, phospholipids and gangliosides of buffalo spermatozoa and seminal plasma showed that there were high levels of polyunsaturated acids. Neutral lipids were the richest in polyunsaturated acids (55% in spermatozoa and 61% in seminal plasma). The major saturated acid of all the principal classes was stearic acid and the major unsaturated acid was docosahexaenoic acid (22:6omega3) except in the neutral lipids in which it was arachidonic acid (20:4omega6). The major aldehyde was palmitaldehyde (16:0) in buffalo sperm lipids and docosanal (22:0) in seminal plasma. More than 50% of the total aldehydes was contributed by aldehydes with a chain length greater than 18 carbon atoms.", "PMID": 957325}
{"id": "pubmed23n0032_2672", "title": "Low temperature preservation of rabbit embryos.", "content": "Rabbit embryos (4-cell, 8-cell and morulae) survived freezing and thawing in the presence of 1-5 M-DMSO. Highest survival rates, assessed by continued development in vitro for 48 to 72 hr, were obtained with cooling rates of 0-74 to 0-90 degrees C/min and thawing rates of approximately 12 and approximately 15 degrees C/min (76%, 83%, 77% and 69% respectively). Transfer of embryos directly to liquid nitrogen after cooling at optimal rates to -110 degrees C instead of -80 degrees C significantly reduced the incidence of rupture of the zona pellucida and mucin coat and increased the rate of recovery. Viability after transfer was low (7% liveborn) and was not correlated with the higher rates of development in vitro to the morula and blastocyst stage.", "contents": "Low temperature preservation of rabbit embryos. Rabbit embryos (4-cell, 8-cell and morulae) survived freezing and thawing in the presence of 1-5 M-DMSO. Highest survival rates, assessed by continued development in vitro for 48 to 72 hr, were obtained with cooling rates of 0-74 to 0-90 degrees C/min and thawing rates of approximately 12 and approximately 15 degrees C/min (76%, 83%, 77% and 69% respectively). Transfer of embryos directly to liquid nitrogen after cooling at optimal rates to -110 degrees C instead of -80 degrees C significantly reduced the incidence of rupture of the zona pellucida and mucin coat and increased the rate of recovery. Viability after transfer was low (7% liveborn) and was not correlated with the higher rates of development in vitro to the morula and blastocyst stage.", "PMID": 957326}
{"id": "pubmed23n0032_2673", "title": "The influence of prostaglandin F-2alpha on pregnenolone metabolism by the autotransplanted ovary of the ewe.", "content": "Eight ewes each with an autotransplanted ovary received infusions of tritium-labelled pregnenolone (41 muCi/hr) for 8 hr into the artery supplying the ovary, together with prostaglandin (PG) F-2alpha (30 mug/hr) for 3 hr beginning 2 hr after the start of the pregnenolone infusion. All animals exhibited oestrus 2-3 days after the start of the experiment. During the PGF-2alpha infusion blood flow through the ovaries was increased by 13%, but subsequently returned to pre-infusion levels. Secretion rates of endogenous progesterone and 20alpha-hydroxypregn-4-en-3-one dropped rapidly 5 hr after the PGF-2alpha infusion had started from values of 250 mug/hr and 25 mug/hr to values below 60 mug/hr and 8 mug/hr, respectively. At this time the conversion of radioactive pregnenolone to progesterone was reduced by 50% of its initial value, but the secretion of endogenous pregnenolone and the formation of radioactive metabolites other than progesterone were not diminished. In 4 control animals, which received pregnenolone only, no changes in ovarian blood flow, steroid secretion rates, or in the conversion of labelled pregnenolone were observed. These results suggest a possible involvement of PGF-2alpha in the regulation of progesterone biosynthesis by an action on the 3beta-hydroxysteroid oxidoreductase-delta5(-4) isomerase enzyme system.", "contents": "The influence of prostaglandin F-2alpha on pregnenolone metabolism by the autotransplanted ovary of the ewe. Eight ewes each with an autotransplanted ovary received infusions of tritium-labelled pregnenolone (41 muCi/hr) for 8 hr into the artery supplying the ovary, together with prostaglandin (PG) F-2alpha (30 mug/hr) for 3 hr beginning 2 hr after the start of the pregnenolone infusion. All animals exhibited oestrus 2-3 days after the start of the experiment. During the PGF-2alpha infusion blood flow through the ovaries was increased by 13%, but subsequently returned to pre-infusion levels. Secretion rates of endogenous progesterone and 20alpha-hydroxypregn-4-en-3-one dropped rapidly 5 hr after the PGF-2alpha infusion had started from values of 250 mug/hr and 25 mug/hr to values below 60 mug/hr and 8 mug/hr, respectively. At this time the conversion of radioactive pregnenolone to progesterone was reduced by 50% of its initial value, but the secretion of endogenous pregnenolone and the formation of radioactive metabolites other than progesterone were not diminished. In 4 control animals, which received pregnenolone only, no changes in ovarian blood flow, steroid secretion rates, or in the conversion of labelled pregnenolone were observed. These results suggest a possible involvement of PGF-2alpha in the regulation of progesterone biosynthesis by an action on the 3beta-hydroxysteroid oxidoreductase-delta5(-4) isomerase enzyme system.", "PMID": 957327}
{"id": "pubmed23n0032_2674", "title": "The relationship between semen evaluation methods and fertility in the bull.", "content": "The results are presented of two experiments in which the quality of samples of bull semen was assessed by a number of laboratory tests and then correlated with the 112-day non-return percentage obtained by the use of those samples in A.I. The results reveal the inadequacy of the laboratory tests used as methods of predicting the fertility of semen samples but show that limits may be set outside which poor semen samples could be discarded.", "contents": "The relationship between semen evaluation methods and fertility in the bull. The results are presented of two experiments in which the quality of samples of bull semen was assessed by a number of laboratory tests and then correlated with the 112-day non-return percentage obtained by the use of those samples in A.I. The results reveal the inadequacy of the laboratory tests used as methods of predicting the fertility of semen samples but show that limits may be set outside which poor semen samples could be discarded.", "PMID": 957328}
{"id": "pubmed23n0032_2675", "title": "Isolation and characterization of the perforatorium of rat spermatozoa.", "content": "The perforatorium of rat spermatozoa was isolated and its protein composition determined. SDS-polyacrylamide gel electrophoresis showed that the organelle is composed of a single polypeptide component with a molecular weight of 13,000. The perforatorium becomes more resistant to solubilization during epididymal transit due to an apparent increase in disulphide bond content. Amino acid analysis of the perforatorium polypeptide revealed a content of 6-5% cysteine.", "contents": "Isolation and characterization of the perforatorium of rat spermatozoa. The perforatorium of rat spermatozoa was isolated and its protein composition determined. SDS-polyacrylamide gel electrophoresis showed that the organelle is composed of a single polypeptide component with a molecular weight of 13,000. The perforatorium becomes more resistant to solubilization during epididymal transit due to an apparent increase in disulphide bond content. Amino acid analysis of the perforatorium polypeptide revealed a content of 6-5% cysteine.", "PMID": 957329}
{"id": "pubmed23n0032_2676", "title": "Effects of oviducal cells on the survival and fertilizing ability of fowl spermatozoa.", "content": "Oviducal epithelial cells were cultured and the effects of the cells on the survival and fertilizing ability of fowl spermatozoa were investigated. When spermatozoa were incubated at 41 degrees C with cells from the infundibulum, shell gland or uterovaginal junction, their motility, assessed at room temperature (20-23 degrees C), was maintained for 5 to 6 days. Fertilizing ability was also retained after culture at 41 degrees C for at least 4 days. During incubation at this temperature, the spermatozoa were almost immotile and rested in close proximity to the oviducal cells. At 23 degrees C, the same spermatozoa were highly motile.", "contents": "Effects of oviducal cells on the survival and fertilizing ability of fowl spermatozoa. Oviducal epithelial cells were cultured and the effects of the cells on the survival and fertilizing ability of fowl spermatozoa were investigated. When spermatozoa were incubated at 41 degrees C with cells from the infundibulum, shell gland or uterovaginal junction, their motility, assessed at room temperature (20-23 degrees C), was maintained for 5 to 6 days. Fertilizing ability was also retained after culture at 41 degrees C for at least 4 days. During incubation at this temperature, the spermatozoa were almost immotile and rested in close proximity to the oviducal cells. At 23 degrees C, the same spermatozoa were highly motile.", "PMID": 957330}
{"id": "pubmed23n0032_2677", "title": "Differentiation of cholesterol compartments in the immature chick testis.", "content": "The behaviour of testicular free and esterified cholesterol during early posthatching development and after stimulation with human chorionic gonadotrophin was studied in the immature chick using combined electron microscope and biochemical techniques. The interstitial cells represented about 65% of the testicular tissue and the lipid droplets in the cytoplasm of these cells were considerably depleted after HCG treatment. The lipid droplets isolated from 7-day-old testis contained about 95% of the total esterified cholesterol which was significantly reduced in the testis and isolated lipid droplets after HCG stimulation. The bulk of free cholesterol was associated with the microsomal fraction whose membranes and cholesterol content increased during normal maturation and after gonadotrophin-induced differentiation.", "contents": "Differentiation of cholesterol compartments in the immature chick testis. The behaviour of testicular free and esterified cholesterol during early posthatching development and after stimulation with human chorionic gonadotrophin was studied in the immature chick using combined electron microscope and biochemical techniques. The interstitial cells represented about 65% of the testicular tissue and the lipid droplets in the cytoplasm of these cells were considerably depleted after HCG treatment. The lipid droplets isolated from 7-day-old testis contained about 95% of the total esterified cholesterol which was significantly reduced in the testis and isolated lipid droplets after HCG stimulation. The bulk of free cholesterol was associated with the microsomal fraction whose membranes and cholesterol content increased during normal maturation and after gonadotrophin-induced differentiation.", "PMID": 957331}
{"id": "pubmed23n0032_2678", "title": "Uterine secretion-like proteins in the seminal plasma of the rabbit.", "content": "The results of polyacrylamide disc electrophoretic, gel chromatographic and agar gel immunoelectrophoretic analyses of rabbit seminal plasma were compared to those for uterine secretions and serum. Two of the seminal plasma proteins seemed to be identical with the uterine proteins uteroglobin and prealbumin.", "contents": "Uterine secretion-like proteins in the seminal plasma of the rabbit. The results of polyacrylamide disc electrophoretic, gel chromatographic and agar gel immunoelectrophoretic analyses of rabbit seminal plasma were compared to those for uterine secretions and serum. Two of the seminal plasma proteins seemed to be identical with the uterine proteins uteroglobin and prealbumin.", "PMID": 957332}
{"id": "pubmed23n0032_2679", "title": "Ovarian blood flow in conscious and anaesthetized pregnant rabbits near term and the influence of arterial blood gas tensions.", "content": "Total ovarian, luteal and stromal blood flows were measured with radioactive microspheres (25 mum diameter) in 7 rabbits anaesthetized with sodium pentobarbitone and 23 conscious rabbits at Day 28 of gestation. Despite major differences in cardiac output, arterial PO2, PCO2, pH and base status, ovarian tissue blood flows were similar in both groups. In the conscious rabbits total ovarian blood flow was negatively related to arterial pressure, and luteal blood flow was negatively related to pressure and arterial pH. In the anaesthetized rabbits ovarian stromal blood flow was positively related to arterial PO2.", "contents": "Ovarian blood flow in conscious and anaesthetized pregnant rabbits near term and the influence of arterial blood gas tensions. Total ovarian, luteal and stromal blood flows were measured with radioactive microspheres (25 mum diameter) in 7 rabbits anaesthetized with sodium pentobarbitone and 23 conscious rabbits at Day 28 of gestation. Despite major differences in cardiac output, arterial PO2, PCO2, pH and base status, ovarian tissue blood flows were similar in both groups. In the conscious rabbits total ovarian blood flow was negatively related to arterial pressure, and luteal blood flow was negatively related to pressure and arterial pH. In the anaesthetized rabbits ovarian stromal blood flow was positively related to arterial PO2.", "PMID": 957334}
{"id": "pubmed23n0032_2680", "title": "The influence of in-vitro culture and cooling on the survival and development of cow embryos.", "content": "Cow morulae cultured in a phosphate-buffered medium containing serum developed normally and retained viability when transferred to recipients. Unlike earlier cleavage stages, cow blastocysts tolerated cooling to 0 degrees C and retained viability after storage for 48 hr at 0 degrees C when transferred to recipients.", "contents": "The influence of in-vitro culture and cooling on the survival and development of cow embryos. Cow morulae cultured in a phosphate-buffered medium containing serum developed normally and retained viability when transferred to recipients. Unlike earlier cleavage stages, cow blastocysts tolerated cooling to 0 degrees C and retained viability after storage for 48 hr at 0 degrees C when transferred to recipients.", "PMID": 957337}
{"id": "pubmed23n0032_2681", "title": "Inhibition of multiple ovulation in hypophysectomized hens treated with gonadotrophins.", "content": "Administration of PMSG or FSH to hypophysectomized hens prevented the multiple ovulation (2-3 ova) induced by an OIH. Inhibition of multiple ovulation did not occur when FSH was injected 1/2-2 hr before the OIH injection. The results are believed to support the theory that withdrawal of FSH stimulation sensitizes a follicle to the OIH in the normal hen.", "contents": "Inhibition of multiple ovulation in hypophysectomized hens treated with gonadotrophins. Administration of PMSG or FSH to hypophysectomized hens prevented the multiple ovulation (2-3 ova) induced by an OIH. Inhibition of multiple ovulation did not occur when FSH was injected 1/2-2 hr before the OIH injection. The results are believed to support the theory that withdrawal of FSH stimulation sensitizes a follicle to the OIH in the normal hen.", "PMID": 957339}
{"id": "pubmed23n0032_2682", "title": "The management of DES-exposed women: one physician's approach.", "content": "The female offspring of DES-treated mothers are examined cytologically and colposcopically annually, starting at the age of seven years no treatment is indicated until a colposcopic atypical transformation zone occurs. With its occurrence all the abnormal epithelium(squamous or columnar) is excised from the vagina and cervix under colposcopic guidance in the hospital or in the office under local anesthesia. The procedures are done in stages to minimize scarring and to eliminate the possibility of vaginal-cervical adhesions or vaginal shortening. The finding of an incidence of 47% of cervical or vaginal intraepithelial neoplasia in 34 patients aged 10 to 26 years so treated to date indicates a high risk of potential candidacy for squamous carcinoma of the vagina or cervix in later years.", "contents": "The management of DES-exposed women: one physician's approach. The female offspring of DES-treated mothers are examined cytologically and colposcopically annually, starting at the age of seven years no treatment is indicated until a colposcopic atypical transformation zone occurs. With its occurrence all the abnormal epithelium(squamous or columnar) is excised from the vagina and cervix under colposcopic guidance in the hospital or in the office under local anesthesia. The procedures are done in stages to minimize scarring and to eliminate the possibility of vaginal-cervical adhesions or vaginal shortening. The finding of an incidence of 47% of cervical or vaginal intraepithelial neoplasia in 34 patients aged 10 to 26 years so treated to date indicates a high risk of potential candidacy for squamous carcinoma of the vagina or cervix in later years.", "PMID": 957347}
{"id": "pubmed23n0032_2683", "title": "Sex ratio in the progeny of mothers with toxemia of pregnancy.", "content": "Maternal-fetal incompatibility may be an essential component in the etiology of toxemia of pregnancy. A prior study found an abnormally high sex (male:female) ratio, suggestuve of Y-dependent histoincompatibility. We studied the records of 373 patients alleged to have some indication of toxemia among 3,246 births and applied standard criteria for classification. There were no significant differences for any group compared with the sexondary sex ratio expected of the population(94.6% Black).", "contents": "Sex ratio in the progeny of mothers with toxemia of pregnancy. Maternal-fetal incompatibility may be an essential component in the etiology of toxemia of pregnancy. A prior study found an abnormally high sex (male:female) ratio, suggestuve of Y-dependent histoincompatibility. We studied the records of 373 patients alleged to have some indication of toxemia among 3,246 births and applied standard criteria for classification. There were no significant differences for any group compared with the sexondary sex ratio expected of the population(94.6% Black).", "PMID": 957354}
{"id": "pubmed23n0032_2684", "title": "Ovarian dysgerminoma: an ultrastructural study.", "content": "A case of ovarian dysgerminoma, found in a 14-year-old girl, is described. The ultrastructural study showed three different types of cells-two kinds of tumor cells(the main tumor cells of the germ-cell type and the second type with deeply indented nuclei and scanty cytoplasm) and stromal cells. The tumor also showed a typical lymphocytic infiltration, and the cells appeared to be embedded in a matrix of collagen fibers and ground substance.", "contents": "Ovarian dysgerminoma: an ultrastructural study. A case of ovarian dysgerminoma, found in a 14-year-old girl, is described. The ultrastructural study showed three different types of cells-two kinds of tumor cells(the main tumor cells of the germ-cell type and the second type with deeply indented nuclei and scanty cytoplasm) and stromal cells. The tumor also showed a typical lymphocytic infiltration, and the cells appeared to be embedded in a matrix of collagen fibers and ground substance.", "PMID": 957355}
{"id": "pubmed23n0032_2685", "title": "Management of cervical neoplasia with colposcopy.", "content": "During a 44-month period the introduction of colposcopy to a metropolitan dysplasia clinic resulted in 1, 144 colposcopic examinations on 442 patients. Agreement between colposcopically directed biopsy and final diagnosis was found in 84%, and directed biopsy revealed the most advanced lesion in 42.5%. Satisfactory colposcopy was performed on 93% of patients, and some degree of histologic abnormality was obtained in 86% of patients with colposcopic abnormalities. Undetected invasive cervical cancer occurred in both cervical conization and colposcopic examinations with equal frequency. Endocervical curettage detected two cases of occult invasive cancer, and increased use of this procedure is recommended. Guidelines for management of cervical neoplasia are suggested.", "contents": "Management of cervical neoplasia with colposcopy. During a 44-month period the introduction of colposcopy to a metropolitan dysplasia clinic resulted in 1, 144 colposcopic examinations on 442 patients. Agreement between colposcopically directed biopsy and final diagnosis was found in 84%, and directed biopsy revealed the most advanced lesion in 42.5%. Satisfactory colposcopy was performed on 93% of patients, and some degree of histologic abnormality was obtained in 86% of patients with colposcopic abnormalities. Undetected invasive cervical cancer occurred in both cervical conization and colposcopic examinations with equal frequency. Endocervical curettage detected two cases of occult invasive cancer, and increased use of this procedure is recommended. Guidelines for management of cervical neoplasia are suggested.", "PMID": 957356}
{"id": "pubmed23n0032_2686", "title": "Intrauterine spontaneous depression of fetal skull: a case report and review of literature.", "content": "Intrauterine depression of fetal skull, with or without fracture, unassociated with any known trauma during pregnancy or delivery, is extremely rare in Western countries though not so rare in Africa among African women. Usually fetal skull depression is caused by forceps or digital pressure of the obstetrician during manual rotation. Forty such cases are reported in the literature-nine in Western countries and the remaining 31 over a period of three years at Harare Hospital Maternity Centre, Salisbury, Rhodesia, Africa. There, an incidence of one in 4,000 deliveries was observed among the African women but none in 6,000 deliveries of European women during the same period at a nearby hospital. The presentation of this paper is made in view of the rarity of intrauterine spontaneous fetal skull depression in Western countries and the not so infrequent occurrence in African and possibly other  developing countries and because of the persistent controversy about the treatment of this condition.", "contents": "Intrauterine spontaneous depression of fetal skull: a case report and review of literature. Intrauterine depression of fetal skull, with or without fracture, unassociated with any known trauma during pregnancy or delivery, is extremely rare in Western countries though not so rare in Africa among African women. Usually fetal skull depression is caused by forceps or digital pressure of the obstetrician during manual rotation. Forty such cases are reported in the literature-nine in Western countries and the remaining 31 over a period of three years at Harare Hospital Maternity Centre, Salisbury, Rhodesia, Africa. There, an incidence of one in 4,000 deliveries was observed among the African women but none in 6,000 deliveries of European women during the same period at a nearby hospital. The presentation of this paper is made in view of the rarity of intrauterine spontaneous fetal skull depression in Western countries and the not so infrequent occurrence in African and possibly other  developing countries and because of the persistent controversy about the treatment of this condition.", "PMID": 957357}
{"id": "pubmed23n0032_2687", "title": "Laminaria augmentation of midtrimester pregnancy termination by intramuscular prostaglandin 15 (S) 15-methyl F2 alpha.", "content": "A group of 15 midtrimester gravid patients underwent intracervical laminaria tent insertion prior to repeated intramuscular injections of 250mu g of prostaglandin 15 (S) 15-methyl F2 alpha at two-hour intervals for elective termination of pregnancy. This group was compared with a previously reported group of 80 patients who had received identical treatment without laminaria insertion. A significant reduction in mean abortion time and an increased 24-hour abortion rate were observed with the addition of laminaria. There was a proportionate decrease in gastrointestinal side effects as well as a reduced need for analgesia in the laminaria group with no complications attributable to the laminaria insertion.", "contents": "Laminaria augmentation of midtrimester pregnancy termination by intramuscular prostaglandin 15 (S) 15-methyl F2 alpha. A group of 15 midtrimester gravid patients underwent intracervical laminaria tent insertion prior to repeated intramuscular injections of 250mu g of prostaglandin 15 (S) 15-methyl F2 alpha at two-hour intervals for elective termination of pregnancy. This group was compared with a previously reported group of 80 patients who had received identical treatment without laminaria insertion. A significant reduction in mean abortion time and an increased 24-hour abortion rate were observed with the addition of laminaria. There was a proportionate decrease in gastrointestinal side effects as well as a reduced need for analgesia in the laminaria group with no complications attributable to the laminaria insertion.", "PMID": 957359}
{"id": "pubmed23n0032_2688", "title": "Three and one-half years' experience with a lower-dose combination oral contraceptive.", "content": "Updated data are presented that span 43 cycles of use of norgestrel 0.030 mg as a lower-dose combination oral contraceptive. Active participation by 1,633 women in 20,312 treatment cycles has resulted in a use-effectiveness rate of 0.13 per 100 woman-years. Satisfactory cycle control, minimal menstrual irregularities and a low incidence of side effects produced high acceptability among users. No drug toxicity was demonstrated. The incidence of discontinuance for medical reasons was low.", "contents": "Three and one-half years' experience with a lower-dose combination oral contraceptive. Updated data are presented that span 43 cycles of use of norgestrel 0.030 mg as a lower-dose combination oral contraceptive. Active participation by 1,633 women in 20,312 treatment cycles has resulted in a use-effectiveness rate of 0.13 per 100 woman-years. Satisfactory cycle control, minimal menstrual irregularities and a low incidence of side effects produced high acceptability among users. No drug toxicity was demonstrated. The incidence of discontinuance for medical reasons was low.", "PMID": 957360}
{"id": "pubmed23n0032_2689", "title": "Female sterilization via minilaparotomy.", "content": "A simple, safe, economical and potentially reversible technique for female sterilization via minilaparotomy is evaluated in 204 patients (not recently pregnant and postpartum). Only light general anesthesia is required, and the procedure is performed with only equipment normally available in any hospital or outpatient clinic. A 2cm circumumbilical or suprapubic incision is made, and the physician manipulates the uterus to bring the fallopian tube in line with the incision. As the cornu passes the incision, the release of intraabdominal pressure at the site of the wound usually pushes the tube out of the peritoneal cavity. A majority of the procedures were performed in less than five minutes. No surgical difficulties were encountered, and no immediate complications or complaints were reported. Patients were usually hospitalized for one or two nights. Early postoperative complications or complaints were reported for 8.4% of the not-recently-pregnant patients and for 4.1% of the postpartum patients at the 7-to-21-day follow-up visit. The most frequently reported complications and complaints were fever and lower abdominal pain. Further studies to determine the effectiveness of this type of sterilization and whether the procedure can be performed safely with local anesthetics and on an outpatient basis are being planned.", "contents": "Female sterilization via minilaparotomy. A simple, safe, economical and potentially reversible technique for female sterilization via minilaparotomy is evaluated in 204 patients (not recently pregnant and postpartum). Only light general anesthesia is required, and the procedure is performed with only equipment normally available in any hospital or outpatient clinic. A 2cm circumumbilical or suprapubic incision is made, and the physician manipulates the uterus to bring the fallopian tube in line with the incision. As the cornu passes the incision, the release of intraabdominal pressure at the site of the wound usually pushes the tube out of the peritoneal cavity. A majority of the procedures were performed in less than five minutes. No surgical difficulties were encountered, and no immediate complications or complaints were reported. Patients were usually hospitalized for one or two nights. Early postoperative complications or complaints were reported for 8.4% of the not-recently-pregnant patients and for 4.1% of the postpartum patients at the 7-to-21-day follow-up visit. The most frequently reported complications and complaints were fever and lower abdominal pain. Further studies to determine the effectiveness of this type of sterilization and whether the procedure can be performed safely with local anesthetics and on an outpatient basis are being planned.", "PMID": 957361}
{"id": "pubmed23n0032_2690", "title": "Steroid delivery systems for contraception.", "content": "Scientific disciplines not normally associated with pharmaceutical development have been employed to enhance the effectiveness and attractiveness of two approaches to steroidal contraception. The use of a bioerodible polymeric carrier for predictable systemic steroidal administration has removed the largest hurdle to the development of a subcutaneous contraceptive implant. A polymeric progesterone delivery system has provided a method for localizing hormonal contraception in the uterine cavity. Extensions of these technologies may provide two useful tools in limiting fertility in an already overcrowded world.", "contents": "Steroid delivery systems for contraception. Scientific disciplines not normally associated with pharmaceutical development have been employed to enhance the effectiveness and attractiveness of two approaches to steroidal contraception. The use of a bioerodible polymeric carrier for predictable systemic steroidal administration has removed the largest hurdle to the development of a subcutaneous contraceptive implant. A polymeric progesterone delivery system has provided a method for localizing hormonal contraception in the uterine cavity. Extensions of these technologies may provide two useful tools in limiting fertility in an already overcrowded world.", "PMID": 957363}
{"id": "pubmed23n0032_2691", "title": "Ethical issues arising from human genetics.", "content": "Advances in understanding genetic disorders have been rapid in the last few years and with them the need and desire for genetic counselling have grown. Almost simultaneously, particularly in the USA, several large screening programmes have been initiated to screen large numbers of people who may be carriers of such deleterious genes as those of Tay-Sachs disease and sickle cell anaemia. The authors of this paper, clinical medical students at University College Hospital, London, spent some time studying the ethical issues raised. The first part of their study, which is not published here, relates to the biochemistry of certain genetic disorders, so leading up to the aspect of the subject which must concern readers of this journal, genetic counselling. At present genetic counselling is generally the province of the medical practitioner working with clinical biochemists, and in this paper their function is described and how programmes of screening for carriers are designed. Whether the subjects of the screening tests are found to be 'innocent' or 'guilty' psychological problems confront them, and of these the genetic counsellor must be aware. In fact the range of ethical problems raised by such counselling is wide and can only be sketched in this article.", "contents": "Ethical issues arising from human genetics. Advances in understanding genetic disorders have been rapid in the last few years and with them the need and desire for genetic counselling have grown. Almost simultaneously, particularly in the USA, several large screening programmes have been initiated to screen large numbers of people who may be carriers of such deleterious genes as those of Tay-Sachs disease and sickle cell anaemia. The authors of this paper, clinical medical students at University College Hospital, London, spent some time studying the ethical issues raised. The first part of their study, which is not published here, relates to the biochemistry of certain genetic disorders, so leading up to the aspect of the subject which must concern readers of this journal, genetic counselling. At present genetic counselling is generally the province of the medical practitioner working with clinical biochemists, and in this paper their function is described and how programmes of screening for carriers are designed. Whether the subjects of the screening tests are found to be 'innocent' or 'guilty' psychological problems confront them, and of these the genetic counsellor must be aware. In fact the range of ethical problems raised by such counselling is wide and can only be sketched in this article.", "PMID": 957367}
{"id": "pubmed23n0032_2692", "title": "Is a moral consensus in medical ethics possible?", "content": "At the moment in Britain and elsewhere the debate inside and outside of Parliament on various medical issues which are essentially moral never ends. Everybody has his own point of view--or principles. But what emerges for society to adopt can often be called in lay terminology 'compromise'. Professor Mitchell argues in this paper that a moral consensus is possible and indeed ought to be achieved, as today the medical practitioner can no longer make his decision only in accordance with the strict code of ethics of the medical profession. The task of the philosopher, says Professor Mitchell, is to interpret the actions and attitudes demanded by modern medical practice.", "contents": "Is a moral consensus in medical ethics possible? At the moment in Britain and elsewhere the debate inside and outside of Parliament on various medical issues which are essentially moral never ends. Everybody has his own point of view--or principles. But what emerges for society to adopt can often be called in lay terminology 'compromise'. Professor Mitchell argues in this paper that a moral consensus is possible and indeed ought to be achieved, as today the medical practitioner can no longer make his decision only in accordance with the strict code of ethics of the medical profession. The task of the philosopher, says Professor Mitchell, is to interpret the actions and attitudes demanded by modern medical practice.", "PMID": 957368}
{"id": "pubmed23n0032_2693", "title": "Limits of confidentiality.", "content": "A patient is described, a woman of 60, who at that age was diagnosed as having Huntington's chorea, a genetic disease which is transmitted as an autosomal dominant. She had one daughter, who was married and lived abroad. The patient's mother and maternal aunt, it emerged during the consultations, had had the disease, and shortly after the diagnosis had been made the patient's sister was admitted to hospital with a repiratory condition, and in the course of treatment Huntington's chorea was diagnosed in her also. She had one daughter. Throughout, the problem was not only that of treating the patient herself, but the situation of all those of the family affected in any way. The patient's husband had not known of her medical history at the time of the marriage. At no time had the daughter been told. The discussion, in which seven doctors, a moral philosopher, and a social worker took part, centered on the basic issue of confidentiality in the case of a disease, from which would stem problems for the affected family through several generations. All were agreed that in these circumstances those affected must be told, but differed as to who should make some of the subsequent decisions. Some thought that having told of the diagnosis and its implications, the doctor should leave further decisions to those concerned; others that in a disease such as Huntington's chorea one is looking at part of a whole chain of genetic material, and, although the individual patient or potential patient is of course important, he or she must be seen in the genetic setting of the community. The participants also considered the psychological.", "contents": "Limits of confidentiality. A patient is described, a woman of 60, who at that age was diagnosed as having Huntington's chorea, a genetic disease which is transmitted as an autosomal dominant. She had one daughter, who was married and lived abroad. The patient's mother and maternal aunt, it emerged during the consultations, had had the disease, and shortly after the diagnosis had been made the patient's sister was admitted to hospital with a repiratory condition, and in the course of treatment Huntington's chorea was diagnosed in her also. She had one daughter. Throughout, the problem was not only that of treating the patient herself, but the situation of all those of the family affected in any way. The patient's husband had not known of her medical history at the time of the marriage. At no time had the daughter been told. The discussion, in which seven doctors, a moral philosopher, and a social worker took part, centered on the basic issue of confidentiality in the case of a disease, from which would stem problems for the affected family through several generations. All were agreed that in these circumstances those affected must be told, but differed as to who should make some of the subsequent decisions. Some thought that having told of the diagnosis and its implications, the doctor should leave further decisions to those concerned; others that in a disease such as Huntington's chorea one is looking at part of a whole chain of genetic material, and, although the individual patient or potential patient is of course important, he or she must be seen in the genetic setting of the community. The participants also considered the psychological.", "PMID": 957370}
{"id": "pubmed23n0032_2694", "title": "The Karen Quinlan case: problems and proposals.", "content": "Karen Quinlan, a young American girl, has lain in hospital since 15 April 1975 without any prospect of recovering consciousness. Her breathing is assisted by means of a respirator and she is fed through a tube inserted in her stomach. Her adoptive parents applied to the courts for permission for the respirator to be switched off. The judge refused permission. Using the Quinlan case as an exemplar, Mr. Kennedy analyses the medical points one by one against the legal background. He would like to see established a code of practice to assist doctors in such cases who at present have no legal guidance. A set of rules arising as a consequence of a series of court decisions would be undesirable; rather a code should be drawn up as the result of discussion between the many people concerned and the consensus so arrived at.", "contents": "The Karen Quinlan case: problems and proposals. Karen Quinlan, a young American girl, has lain in hospital since 15 April 1975 without any prospect of recovering consciousness. Her breathing is assisted by means of a respirator and she is fed through a tube inserted in her stomach. Her adoptive parents applied to the courts for permission for the respirator to be switched off. The judge refused permission. Using the Quinlan case as an exemplar, Mr. Kennedy analyses the medical points one by one against the legal background. He would like to see established a code of practice to assist doctors in such cases who at present have no legal guidance. A set of rules arising as a consequence of a series of court decisions would be undesirable; rather a code should be drawn up as the result of discussion between the many people concerned and the consensus so arrived at.", "PMID": 957371}
{"id": "pubmed23n0032_2695", "title": "Diaphragmatic hernia in the south-west of England.", "content": "A retrospective anatomical, family, and epidemiological study was made of 143 patients (81 female and 62 male) with diaphragmatic hernia who were born in the south-west of England between 1943 and 1974. Thirty-nine cases were stillborn. Seventy-five per cent of patients had a left-sided diaphragmatic defect, 22% had a right-sided defect, and 3% had a bilateral defect. Fifty per cent of the patients had other congenital malformations, most frequently of the nervous system. No maternal age or birth order effect was noted. Cases of diaphragmatic hernia without other malformations had in general a normal fetal growth rate. Eight per cent of the cases were illegitimate. There were two pairs of twins discordant for diaphragmatic hernia, one pair being dizygotic and the other monozygotic. In no case of diaphragmatic hernia was there a relative affected with a diaphragmatic hernia. The most common type of diaphragmatic defect was a posterolateral hernia (92%), followed in frequency by an eventration of the diaphragm (5%), the least common defect being a retrocostosternal hernia (2%). Diaphragmatic hernia appears to be aetiologically as well as anatomically heterogeneous. In this series there were two cases of trisomy 18, one case of trisomy 21, one case trisomic for a small part of chromosome 20, and two cases with the Pierre Robin syndrome. It seems likely that diaphragmatic hernia is a non-specific consequence of several teratological processes.", "contents": "Diaphragmatic hernia in the south-west of England. A retrospective anatomical, family, and epidemiological study was made of 143 patients (81 female and 62 male) with diaphragmatic hernia who were born in the south-west of England between 1943 and 1974. Thirty-nine cases were stillborn. Seventy-five per cent of patients had a left-sided diaphragmatic defect, 22% had a right-sided defect, and 3% had a bilateral defect. Fifty per cent of the patients had other congenital malformations, most frequently of the nervous system. No maternal age or birth order effect was noted. Cases of diaphragmatic hernia without other malformations had in general a normal fetal growth rate. Eight per cent of the cases were illegitimate. There were two pairs of twins discordant for diaphragmatic hernia, one pair being dizygotic and the other monozygotic. In no case of diaphragmatic hernia was there a relative affected with a diaphragmatic hernia. The most common type of diaphragmatic defect was a posterolateral hernia (92%), followed in frequency by an eventration of the diaphragm (5%), the least common defect being a retrocostosternal hernia (2%). Diaphragmatic hernia appears to be aetiologically as well as anatomically heterogeneous. In this series there were two cases of trisomy 18, one case of trisomy 21, one case trisomic for a small part of chromosome 20, and two cases with the Pierre Robin syndrome. It seems likely that diaphragmatic hernia is a non-specific consequence of several teratological processes.", "PMID": 957374}
{"id": "pubmed23n0032_2696", "title": "Syndrome designations.", "content": "Because syndrome designations permit the collection of data, they are much more than just lables. As new syndromes become delineated, their names connote (1) their phenotypic spectra, (2) their natural histories, and (3) their modes of inheritance or risk of recurrence. Various methods for designating new syndromes are reviewed, including naming them after (1) the basic defect, (2) an eponym, (3) one or more striking features, (4) an acronym, (5) a numeral, (6) a geographic term, and (7) some combination of the above. None of these systems of nomenclature is without fault. The advantages and disadvantages of each are discussed.", "contents": "Syndrome designations. Because syndrome designations permit the collection of data, they are much more than just lables. As new syndromes become delineated, their names connote (1) their phenotypic spectra, (2) their natural histories, and (3) their modes of inheritance or risk of recurrence. Various methods for designating new syndromes are reviewed, including naming them after (1) the basic defect, (2) an eponym, (3) one or more striking features, (4) an acronym, (5) a numeral, (6) a geographic term, and (7) some combination of the above. None of these systems of nomenclature is without fault. The advantages and disadvantages of each are discussed.", "PMID": 957375}
{"id": "pubmed23n0032_2697", "title": "Pfeiffer syndrome: report of a family and review of the literature.", "content": "A 5-year-old boy and his father with Pfeiffer syndrome are described. They had acrocephaly, hypertelorism, antimongoloid slant of the palpebral fissures, protrusion of the eyes, large and broad nose, small mandible, irregularly placed teeth, additional upper canine, high-arched palate, partial syndactyly of fingers and toes, brachydactyly of toes, valgus deformity of hypertrophied triangular great toes, broad phalanges of the great toes and broad first metatarsals, accessory epiphyses lateral to the interphalangeal joint of the great toes, and normal intelligence. To our knowledge, this is the first family in which the syndrome is almost totally confined to the head and feet--it spares the upper limbs except for partial skin syndactyly between the fingers--and the third family showing inheritance through three successive generations suggesting an autosomal dominant mode of inheritance. The published papers are reviewed and the clinical and x-ray signs are tabulated.", "contents": "Pfeiffer syndrome: report of a family and review of the literature. A 5-year-old boy and his father with Pfeiffer syndrome are described. They had acrocephaly, hypertelorism, antimongoloid slant of the palpebral fissures, protrusion of the eyes, large and broad nose, small mandible, irregularly placed teeth, additional upper canine, high-arched palate, partial syndactyly of fingers and toes, brachydactyly of toes, valgus deformity of hypertrophied triangular great toes, broad phalanges of the great toes and broad first metatarsals, accessory epiphyses lateral to the interphalangeal joint of the great toes, and normal intelligence. To our knowledge, this is the first family in which the syndrome is almost totally confined to the head and feet--it spares the upper limbs except for partial skin syndactyly between the fingers--and the third family showing inheritance through three successive generations suggesting an autosomal dominant mode of inheritance. The published papers are reviewed and the clinical and x-ray signs are tabulated.", "PMID": 957376}
{"id": "pubmed23n0032_2698", "title": "Ectrodactyly-ectodermal dysplasia-clefting (EEC) syndrome: dominant inheritance and variable expression.", "content": "An infant is reported with a complete form of the ectrodactyly-ectodermal dysplasia clefting (EEC) syndrome, inherited from his mother, who has a partial expression of the condition, without clefting. This observation stresses the phenotypic variability of the EEC syndrome, which in most cases is inherited as an autosomal dominant with reduced penetrance.", "contents": "Ectrodactyly-ectodermal dysplasia-clefting (EEC) syndrome: dominant inheritance and variable expression. An infant is reported with a complete form of the ectrodactyly-ectodermal dysplasia clefting (EEC) syndrome, inherited from his mother, who has a partial expression of the condition, without clefting. This observation stresses the phenotypic variability of the EEC syndrome, which in most cases is inherited as an autosomal dominant with reduced penetrance.", "PMID": 957377}
{"id": "pubmed23n0032_2699", "title": "Chronic spinal muscular atrophy of facioscapulohumeral type.", "content": "Chronic spinal muscular atrophy of FSH type affecting a mother and her son and daughter is reported. The relevant literature is reviewed and the relation between this conditon and Kugelberg-Welander (K-W) disease is discussed. Chronic spinal muscular atrophy of FSH type is considered to be a different entity from the eponymous K-W disease. Each type of muscular dystrophy, e.g. limb-girdle, FSH, distal, ocular, or oculopharyngeal type, has its counterpart of nuclear origin. A classification of the chronic spinal muscular atrophies is suggested following the classification of muscular dystrophy.", "contents": "Chronic spinal muscular atrophy of facioscapulohumeral type. Chronic spinal muscular atrophy of FSH type affecting a mother and her son and daughter is reported. The relevant literature is reviewed and the relation between this conditon and Kugelberg-Welander (K-W) disease is discussed. Chronic spinal muscular atrophy of FSH type is considered to be a different entity from the eponymous K-W disease. Each type of muscular dystrophy, e.g. limb-girdle, FSH, distal, ocular, or oculopharyngeal type, has its counterpart of nuclear origin. A classification of the chronic spinal muscular atrophies is suggested following the classification of muscular dystrophy.", "PMID": 957378}
{"id": "pubmed23n0032_2700", "title": "Alkaline phosphatase activity in cultured skin fibroblasts from fibrodysplasia ossificans progressiva.", "content": "Alkaline phosphatase activity in four strains of cultured skin fibroblasts obtained from a patient with fibrodysplasia ossificans progressiva was at the low normal range. The enzyme activity in normal fibroblasts significantly increased at late confluency. It appears that the high levels of alkaline phosphatase activity reported in biopsies of lesions are not genetically determined but are secondary events of local tissue reaction.", "contents": "Alkaline phosphatase activity in cultured skin fibroblasts from fibrodysplasia ossificans progressiva. Alkaline phosphatase activity in four strains of cultured skin fibroblasts obtained from a patient with fibrodysplasia ossificans progressiva was at the low normal range. The enzyme activity in normal fibroblasts significantly increased at late confluency. It appears that the high levels of alkaline phosphatase activity reported in biopsies of lesions are not genetically determined but are secondary events of local tissue reaction.", "PMID": 957379}
{"id": "pubmed23n0032_2701", "title": "Topographic approach for analysis of palm crease variants.", "content": "As the variability and possible clinical significance of palm increase abnormalities receive greater attention, an accurate and objective method for evaluating the palm increase variants is required. A new method is described employing a topographic approach. A network of co-ordinates drawn on the palm prints enables an accurate and reproducible description to be made of unlimited numbers of variants of palm crease configurations. This method makes possible quantitative studies rather than merely qualitative descriptions of the palm crease patterns. To determine its usefulness, this method was employed in a comparative study of 100 individuals with Down's syndrome and 100 controls. Significantly higher total degree of transversality (T-DoT), as defined in this paper, was found in the Down's syndrome group. T-DoT may be a useful parameter in the evaluation of crease patterns of patients with congenital and genetic disorders.", "contents": "Topographic approach for analysis of palm crease variants. As the variability and possible clinical significance of palm increase abnormalities receive greater attention, an accurate and objective method for evaluating the palm increase variants is required. A new method is described employing a topographic approach. A network of co-ordinates drawn on the palm prints enables an accurate and reproducible description to be made of unlimited numbers of variants of palm crease configurations. This method makes possible quantitative studies rather than merely qualitative descriptions of the palm crease patterns. To determine its usefulness, this method was employed in a comparative study of 100 individuals with Down's syndrome and 100 controls. Significantly higher total degree of transversality (T-DoT), as defined in this paper, was found in the Down's syndrome group. T-DoT may be a useful parameter in the evaluation of crease patterns of patients with congenital and genetic disorders.", "PMID": 957380}
{"id": "pubmed23n0032_2702", "title": "Further studies on the genetic heterogeneity of cebocephaly.", "content": "The detailed morphological description of 4 cases with cebocephaly, 3 of which were karotyped (one with D trisomy and 2 with normal karyotypes), are presented. Analysis of all cytogenetically studied cases with this malformation reveals that cebocephaly with a normal karyotype may result from more than one mutant gene, and so it may be accompanied by different extracranial abnormalities. On the other hand an absence of visceral malformations does not exclude chromosomal aberrations; thus 18p- syndrome, where cebocephaly is frequent, may have no visceral abnormalities.", "contents": "Further studies on the genetic heterogeneity of cebocephaly. The detailed morphological description of 4 cases with cebocephaly, 3 of which were karotyped (one with D trisomy and 2 with normal karyotypes), are presented. Analysis of all cytogenetically studied cases with this malformation reveals that cebocephaly with a normal karyotype may result from more than one mutant gene, and so it may be accompanied by different extracranial abnormalities. On the other hand an absence of visceral malformations does not exclude chromosomal aberrations; thus 18p- syndrome, where cebocephaly is frequent, may have no visceral abnormalities.", "PMID": 957381}
{"id": "pubmed23n0032_2703", "title": "Non-disjunction of an unusual X chromosome.", "content": "Because of multiple abnormalities in her children, a young mother was investigated and shown to have a 47,XXX chromosome constitution. Additional C group chromosomes without visible centromeric constrictions were found in a number of cells from the peripheral blood, and using C and Q banding techniques these chromosomes were identified as X chromosomes. Analysis of the banding karyotypes of 300 cells revealed that the acentric X chromosomes had the ability to replicate and that this replication was associated with non-disjunction leading to aneuploid cells. Even though cultured skin cells did not have acentric or extra chromosomes in addition to the triple-X, examination of buccal mucosa cells for the presence of X-bodies suggested that the phenomenon of non-disjunction was present in the epithelial cells of the patient. In addition to the X without a visible centromeric constriction, either acentric D or E chromosomes were found. The data suggest that a functional defect in the cells per se is responsible for the appearance of the acentric chromosomes.", "contents": "Non-disjunction of an unusual X chromosome. Because of multiple abnormalities in her children, a young mother was investigated and shown to have a 47,XXX chromosome constitution. Additional C group chromosomes without visible centromeric constrictions were found in a number of cells from the peripheral blood, and using C and Q banding techniques these chromosomes were identified as X chromosomes. Analysis of the banding karyotypes of 300 cells revealed that the acentric X chromosomes had the ability to replicate and that this replication was associated with non-disjunction leading to aneuploid cells. Even though cultured skin cells did not have acentric or extra chromosomes in addition to the triple-X, examination of buccal mucosa cells for the presence of X-bodies suggested that the phenomenon of non-disjunction was present in the epithelial cells of the patient. In addition to the X without a visible centromeric constriction, either acentric D or E chromosomes were found. The data suggest that a functional defect in the cells per se is responsible for the appearance of the acentric chromosomes.", "PMID": 957382}
{"id": "pubmed23n0032_2704", "title": "Multiple congenital defects associated with trisomy for long arm of No. 4.", "content": "The clinical and cytogenetic findings of a male infant with multiple congenital anomalies and trisomy for the distal third of the long arm of No. 4 are described. The abnormal chromosome was inherited from the mother who had a balanced translocation, t(4;9)(q31;q34). Trisomy for the long arm of No. 4 has previously been described in only 3 patients.", "contents": "Multiple congenital defects associated with trisomy for long arm of No. 4. The clinical and cytogenetic findings of a male infant with multiple congenital anomalies and trisomy for the distal third of the long arm of No. 4 are described. The abnormal chromosome was inherited from the mother who had a balanced translocation, t(4;9)(q31;q34). Trisomy for the long arm of No. 4 has previously been described in only 3 patients.", "PMID": 957383}
{"id": "pubmed23n0032_2705", "title": "Tetraploidy in a liveborn infant.", "content": "A congenitally malformed infant with a tetraploid chromosome complement who survived to 1 year of age is reported. The relationship of the polyploidy and the anomalies is discussed.", "contents": "Tetraploidy in a liveborn infant. A congenitally malformed infant with a tetraploid chromosome complement who survived to 1 year of age is reported. The relationship of the polyploidy and the anomalies is discussed.", "PMID": 957384}
{"id": "pubmed23n0032_2706", "title": "46,XY/46,XY,21q- mosaicism in an infant with neutropenia and properdin deficiency.", "content": "An infant with neutropenia, properdin deficiency, and a 46,XY/46,XY,21q- mosaicism is described. It is not known whether these two findings are related to the missing 21q material. The propositus is normal in appearance, and has none of the phenotypic features associated with the G-group deletion syndromes.", "contents": "46,XY/46,XY,21q- mosaicism in an infant with neutropenia and properdin deficiency. An infant with neutropenia, properdin deficiency, and a 46,XY/46,XY,21q- mosaicism is described. It is not known whether these two findings are related to the missing 21q material. The propositus is normal in appearance, and has none of the phenotypic features associated with the G-group deletion syndromes.", "PMID": 957385}
{"id": "pubmed23n0032_2707", "title": "Graduate medical education viewed from the national intern and resident matching program.", "content": "The total number of applicants for first-year programs in graduate medical education through the National Intern and Resident Matching Program in 1976 exceeded the number of positions offered for the second consecutive year. The number of positions available for U.S. medical school graduates in 1976 was approximately 1.22. Based on student preferences for their first-year graduate medical education programs, there were deficits in the number of openings offered in the primary care specialties and surfeits in medical and surgical subspecialties, pathology, psychiatry, and radiology.", "contents": "Graduate medical education viewed from the national intern and resident matching program. The total number of applicants for first-year programs in graduate medical education through the National Intern and Resident Matching Program in 1976 exceeded the number of positions offered for the second consecutive year. The number of positions available for U.S. medical school graduates in 1976 was approximately 1.22. Based on student preferences for their first-year graduate medical education programs, there were deficits in the number of openings offered in the primary care specialties and surfeits in medical and surgical subspecialties, pathology, psychiatry, and radiology.", "PMID": 957386}
{"id": "pubmed23n0032_2708", "title": "The mystification of meaning: doctor-patient encounters.", "content": "The symbolic and communicative aspects of illness and treatment are examined. A number of factors that complicate interpersonal communication are identified and found to exist in their most extreme form in medical settings. New directions in research and training are suggested that might prepare medical personnel to respond more sensitively and flexibly to the symbolic aspects of illness.", "contents": "The mystification of meaning: doctor-patient encounters. The symbolic and communicative aspects of illness and treatment are examined. A number of factors that complicate interpersonal communication are identified and found to exist in their most extreme form in medical settings. New directions in research and training are suggested that might prepare medical personnel to respond more sensitively and flexibly to the symbolic aspects of illness.", "PMID": 957387}
{"id": "pubmed23n0032_2709", "title": "Marital satisfaction as perceived by the medical student's spouse.", "content": "This is a report of student marriages as perceived by spouses of medical students at the Southwestern Medical School, University of Texas Health Science Center at Dallas. Between August and November 1974 a questionnaire was sent to all spouses (239) of sophomore, junior, and senior students and 1974 graduates. The questionnaire examined: areas of stress within marriage, congruence between expected and actual behavior in the marital partner's role performance, and the spouse's attitudes toward marital counseling and whether a marital counseling service should be made available. Stress factors frequently cited were student's preoccupation due to school pressures, limited finances for recreation and nonessentials, student's inaccessibility due to school and loneliness. Most respondents stated satisfaction with their marriage; however, 38 percent indicated that their marriage could have benefited from counseling some time during medical school.", "contents": "Marital satisfaction as perceived by the medical student's spouse. This is a report of student marriages as perceived by spouses of medical students at the Southwestern Medical School, University of Texas Health Science Center at Dallas. Between August and November 1974 a questionnaire was sent to all spouses (239) of sophomore, junior, and senior students and 1974 graduates. The questionnaire examined: areas of stress within marriage, congruence between expected and actual behavior in the marital partner's role performance, and the spouse's attitudes toward marital counseling and whether a marital counseling service should be made available. Stress factors frequently cited were student's preoccupation due to school pressures, limited finances for recreation and nonessentials, student's inaccessibility due to school and loneliness. Most respondents stated satisfaction with their marriage; however, 38 percent indicated that their marriage could have benefited from counseling some time during medical school.", "PMID": 957388}
{"id": "pubmed23n0032_2710", "title": "Six years of experience with a summer program for minority students.", "content": "This artical summarizes six years of experience of the University of Texas Medical Branch at Galveston (UTMB) with a summer program for the recruitment of minority students. During the six years, UTMB conducted six summer experiences for 112 Mexican-American and black premedical students and made numerous contacts with undergraduate college faculty and administrative staff for the purpose of increasing minority student enrollment in medical schools in general and in UTMB in particular. The program, which emphasized experiences which would increase the potential student's motivations, information, and academic skills, has resulted in numerous minority students entering medical school; but the impact on UTMB enrollments has been disappointing. Implications of the results of the program on future efforts to recruit minority students are discussed.", "contents": "Six years of experience with a summer program for minority students. This artical summarizes six years of experience of the University of Texas Medical Branch at Galveston (UTMB) with a summer program for the recruitment of minority students. During the six years, UTMB conducted six summer experiences for 112 Mexican-American and black premedical students and made numerous contacts with undergraduate college faculty and administrative staff for the purpose of increasing minority student enrollment in medical schools in general and in UTMB in particular. The program, which emphasized experiences which would increase the potential student's motivations, information, and academic skills, has resulted in numerous minority students entering medical school; but the impact on UTMB enrollments has been disappointing. Implications of the results of the program on future efforts to recruit minority students are discussed.", "PMID": 957389}
{"id": "pubmed23n0032_2711", "title": "Training medical students in interpersonal relationship skills.", "content": "A review of the literature on training medical students in interviewing skills reveals a neglect of experimentally controlled studies as well as methods based on the most thoroughly researched approach to training, the Rogerian \"core facilitative qualities.\" This paper reports a description and experimental evaluation of a training method designed to increase the student's comfort in dealing with emotionally intense material and to increase his skills in the core facilitative qualities. Comparison of the experimental and control groups reveals a training effect on the student's ability to recognize and communicate empathic understanding and on attitude and personality variables.", "contents": "Training medical students in interpersonal relationship skills. A review of the literature on training medical students in interviewing skills reveals a neglect of experimentally controlled studies as well as methods based on the most thoroughly researched approach to training, the Rogerian \"core facilitative qualities.\" This paper reports a description and experimental evaluation of a training method designed to increase the student's comfort in dealing with emotionally intense material and to increase his skills in the core facilitative qualities. Comparison of the experimental and control groups reveals a training effect on the student's ability to recognize and communicate empathic understanding and on attitude and personality variables.", "PMID": 957390}
{"id": "pubmed23n0032_2712", "title": "Comparison of the psychiatry learning system and traditional teaching of psychiatry.", "content": "Pressures of time, changing curricula, and increased enrollment in medical schools require the development of more efficient teaching methods to replace or supplement the traditional lecture format. The faculty of the Department of Psychiatry and Behavioral Sciences at the Medical University of South Carolina has developed an individualized course in basic psychiatry which can replace approximately 50 hours of lectures ordinarily delivered to students during the rotation on clinical psychiatry. The individualized course, referred to as the Psychiatry Learning System (PLS), was received by half the class during the year 1973-74, and the lecture course was delivered to the other half. These two groups were compared on several learning outcomes, including attainment of theoretical knowledge about psychiatry, observational skills, interviewing ability, and problem-solving ability. Overall performance ratings by clinical supervisors were also obtained. The PLS group scored no worse on any of the measures but statistically better on half of them than did the lecture group.", "contents": "Comparison of the psychiatry learning system and traditional teaching of psychiatry. Pressures of time, changing curricula, and increased enrollment in medical schools require the development of more efficient teaching methods to replace or supplement the traditional lecture format. The faculty of the Department of Psychiatry and Behavioral Sciences at the Medical University of South Carolina has developed an individualized course in basic psychiatry which can replace approximately 50 hours of lectures ordinarily delivered to students during the rotation on clinical psychiatry. The individualized course, referred to as the Psychiatry Learning System (PLS), was received by half the class during the year 1973-74, and the lecture course was delivered to the other half. These two groups were compared on several learning outcomes, including attainment of theoretical knowledge about psychiatry, observational skills, interviewing ability, and problem-solving ability. Overall performance ratings by clinical supervisors were also obtained. The PLS group scored no worse on any of the measures but statistically better on half of them than did the lecture group.", "PMID": 957391}
{"id": "pubmed23n0032_2713", "title": "Behavioral self-analysis in the medical curriculum.", "content": "Medical students in a first-year behavioral science course were required to conduct a behavior change project in accordance with a model based upon behavioristic principles. Most students conducted projects designed to alter their own behaviors, such as studying, eating, sleeping, and exercising. Student feedback, as well as formal evaluation of the projects that were conducted, provided a basis for the view that such procedures can make a significant contribution to the teaching of behavioral change technology in the medical curriculum.", "contents": "Behavioral self-analysis in the medical curriculum. Medical students in a first-year behavioral science course were required to conduct a behavior change project in accordance with a model based upon behavioristic principles. Most students conducted projects designed to alter their own behaviors, such as studying, eating, sleeping, and exercising. Student feedback, as well as formal evaluation of the projects that were conducted, provided a basis for the view that such procedures can make a significant contribution to the teaching of behavioral change technology in the medical curriculum.", "PMID": 957392}
{"id": "pubmed23n0032_2714", "title": "Problems and concerns among medical students--1975.", "content": "Students at the University of Colorado School of Medicine were surveyed using an inventory of problems and conerns relating to their personal and academic lives. Results were to provide a data base for improvement of their educational environment via the ecosystem design process. The survey revealed that chief among student conerns are a lack of personal freedom, excessive academic pressures, and feelings of dehumanization. A marked trend toward increasing difficulty with thoughts of having chosen the wrong profession is noted over the four class levels. The authors offer several hypotheses relating to the underlying conditions responsible for these findings and discuss their possible relation to the current malpractice insurance crisis.", "contents": "Problems and concerns among medical students--1975. Students at the University of Colorado School of Medicine were surveyed using an inventory of problems and conerns relating to their personal and academic lives. Results were to provide a data base for improvement of their educational environment via the ecosystem design process. The survey revealed that chief among student conerns are a lack of personal freedom, excessive academic pressures, and feelings of dehumanization. A marked trend toward increasing difficulty with thoughts of having chosen the wrong profession is noted over the four class levels. The authors offer several hypotheses relating to the underlying conditions responsible for these findings and discuss their possible relation to the current malpractice insurance crisis.", "PMID": 957400}
{"id": "pubmed23n0032_2715", "title": "Prediction of screening decisions in a medical school admission process.", "content": "To study and predict a set of screening decisions in a medical school admission process, a stratified sample (n = 864) was drawn to represent the range of applicants to the Yale University School of Medicine during a single year. A smaller sample from the following year's applicants was drawn in a similar fashion for purposes of cross-validation. Prior to the prediction of screening decisions, a set of independent variables was selected by a factor analytic procedure from credentials in an applicant's admission folder. These folder variables ranged in nature from quantitative measures of academic performance to demographic information and types of extracurricular activity. Two multivariate statistical procedures, Sonquist's Automatic Interaction Detection (AID) and linear discriminant analysis (LDA), were used to predict screening decisions. Measures of academic performance and ability proved to be the most effective predictors of screening decision, as evidenced in the final AID tree and the discriminant function.", "contents": "Prediction of screening decisions in a medical school admission process. To study and predict a set of screening decisions in a medical school admission process, a stratified sample (n = 864) was drawn to represent the range of applicants to the Yale University School of Medicine during a single year. A smaller sample from the following year's applicants was drawn in a similar fashion for purposes of cross-validation. Prior to the prediction of screening decisions, a set of independent variables was selected by a factor analytic procedure from credentials in an applicant's admission folder. These folder variables ranged in nature from quantitative measures of academic performance to demographic information and types of extracurricular activity. Two multivariate statistical procedures, Sonquist's Automatic Interaction Detection (AID) and linear discriminant analysis (LDA), were used to predict screening decisions. Measures of academic performance and ability proved to be the most effective predictors of screening decision, as evidenced in the final AID tree and the discriminant function.", "PMID": 957401}
{"id": "pubmed23n0032_2716", "title": "The Medical Polyclinic: an approach to conflicting needs in a teaching hospital.", "content": "University teaching hospitals have become increasingly aware of their responsibility to improve both the teaching of ambulatory care and the quality of care provided in their clinics. This paper describes how one department of medicine met this challenge by forming a \"Medical Polyclinic.\" The majority of the department's faculty and house staff, at all academic and training levels, participate in a system of ambulatory care with the following objectives: each patient has a single physician whom he sees by appointment and who coordinates his care; all medical subspecialties are available in the same clinic session; the clinic is attractive and efficient. While these goals are not infrequently met in private group practices, they are unusual in a university teaching hospital, where faculty, house staff, students, and patients each have unique needs, not always compatible. The success and problems of the polyclinic approach are discussed.", "contents": "The Medical Polyclinic: an approach to conflicting needs in a teaching hospital. University teaching hospitals have become increasingly aware of their responsibility to improve both the teaching of ambulatory care and the quality of care provided in their clinics. This paper describes how one department of medicine met this challenge by forming a \"Medical Polyclinic.\" The majority of the department's faculty and house staff, at all academic and training levels, participate in a system of ambulatory care with the following objectives: each patient has a single physician whom he sees by appointment and who coordinates his care; all medical subspecialties are available in the same clinic session; the clinic is attractive and efficient. While these goals are not infrequently met in private group practices, they are unusual in a university teaching hospital, where faculty, house staff, students, and patients each have unique needs, not always compatible. The success and problems of the polyclinic approach are discussed.", "PMID": 957402}
{"id": "pubmed23n0032_2717", "title": "Systematic instruction in interpretive aspects of laboratory medicine.", "content": "It is increasingly important for physicians to develop competence in ordering and interpreting laboratory tests. A review of the literature revealed no formal discussion of systematic instruction in the interpretive aspects of laboratory medicine. The goals, organization, instructional approaches, and assessment of a course offering such instruction are described here. Students are expected to develop an algorithmic approach to ordering and interpreting laboratory tests in the course of diagnosing and managing clinical problems in the major areas of medicine. Instructional approaches include lectures, case problem-solving sessions, and independent study. There has been an exponential growth in the number of students electing to take this course. Students respond enthusiastically to most aspects of the course. They are rated significantly higher on appropriate criterion measures in subsequent clinical electives than students who have not had such formal instruction in laboratory medicine during the senior years.", "contents": "Systematic instruction in interpretive aspects of laboratory medicine. It is increasingly important for physicians to develop competence in ordering and interpreting laboratory tests. A review of the literature revealed no formal discussion of systematic instruction in the interpretive aspects of laboratory medicine. The goals, organization, instructional approaches, and assessment of a course offering such instruction are described here. Students are expected to develop an algorithmic approach to ordering and interpreting laboratory tests in the course of diagnosing and managing clinical problems in the major areas of medicine. Instructional approaches include lectures, case problem-solving sessions, and independent study. There has been an exponential growth in the number of students electing to take this course. Students respond enthusiastically to most aspects of the course. They are rated significantly higher on appropriate criterion measures in subsequent clinical electives than students who have not had such formal instruction in laboratory medicine during the senior years.", "PMID": 957403}
{"id": "pubmed23n0032_2718", "title": "Validity and use of written simulation tests of clinical performance.", "content": "The outcome of a simulated clinical problem (drug-induced agranulocytosis) was compared with the outcome of similar cases in real medical service. The total performance of 100 sixth-year medical students at the end of their clerkship in internal medicine resulted in a mortality of 10 per cent in the simulation exercise. This mortality rate is the same as that which occured in the medical service during a four-year period. The total performance of the students was at about the same level at the end of the first clinical term and at the beginning of the clerkship in internal medicine (sixth term) but significantly better at the end of the latter.", "contents": "Validity and use of written simulation tests of clinical performance. The outcome of a simulated clinical problem (drug-induced agranulocytosis) was compared with the outcome of similar cases in real medical service. The total performance of 100 sixth-year medical students at the end of their clerkship in internal medicine resulted in a mortality of 10 per cent in the simulation exercise. This mortality rate is the same as that which occured in the medical service during a four-year period. The total performance of the students was at about the same level at the end of the first clinical term and at the beginning of the clerkship in internal medicine (sixth term) but significantly better at the end of the latter.", "PMID": 957404}
{"id": "pubmed23n0032_2719", "title": "The results of a 13-year training program in maternal and child health for physicians.", "content": "This paper describes the 13-year experience of one school of public health in preparing physicians for the field of maternal and child health. Some 256 (87.7 percent) of the 292 graduates have remained in community health work. Of the 292 graduates, 106 (36.3 percent) are engaged in some teaching responsibilities.", "contents": "The results of a 13-year training program in maternal and child health for physicians. This paper describes the 13-year experience of one school of public health in preparing physicians for the field of maternal and child health. Some 256 (87.7 percent) of the 292 graduates have remained in community health work. Of the 292 graduates, 106 (36.3 percent) are engaged in some teaching responsibilities.", "PMID": 957405}
{"id": "pubmed23n0032_2720", "title": "Acinetobacter and similar organisms in ear infections.", "content": "Fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. After comparison with eight reference strains, 32 of the isolates were identified as Acinetobacter anitratus, 22 as Acinetobacter Iwoffii, three as Moraxella spp. and one as Achromobacter sp. Analysis of the clinical findings suggests that although most of these organisms played little part in the disease process, a few strains were probably pathogenic in this situation.", "contents": "Acinetobacter and similar organisms in ear infections. Fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. After comparison with eight reference strains, 32 of the isolates were identified as Acinetobacter anitratus, 22 as Acinetobacter Iwoffii, three as Moraxella spp. and one as Achromobacter sp. Analysis of the clinical findings suggests that although most of these organisms played little part in the disease process, a few strains were probably pathogenic in this situation.", "PMID": 957420}
{"id": "pubmed23n0032_2721", "title": "The detection and avoidance of false-positive reactions in tests for rubella-specific IgM.", "content": "Serum specimens tested for rubella-specific IgM by the haemagglutination-inhibition technique may give false-positive results due to (a) storage at -20 degrees C, (b) bacterial contamination, or (c) inactivation at 56 degrees C. These false-positive reactions can be distinguished from rubella-specific IgM activity by their resistance to 2-ME.", "contents": "The detection and avoidance of false-positive reactions in tests for rubella-specific IgM. Serum specimens tested for rubella-specific IgM by the haemagglutination-inhibition technique may give false-positive results due to (a) storage at -20 degrees C, (b) bacterial contamination, or (c) inactivation at 56 degrees C. These false-positive reactions can be distinguished from rubella-specific IgM activity by their resistance to 2-ME.", "PMID": 957421}
{"id": "pubmed23n0032_2722", "title": "Anatomy and ultrastructure of the excretory system of the lizard, Sceloporus cyanogenys.", "content": "The anatomy and ultrastructure of the lizard kidney (Sceloporus cyanogenys) have been studied by light and electron microscopy. The number of glomeruli was counted in serial sections and estimated to be 2,000 (in the two kidneys). Beginning with the glomerulus and Bowman's capsule the nephron segments are sequentially: (a) proximal tubule; (b) intermediate ciliated segment consisting of a proximal and distal part; (c) distal tubule, which can be divided into two segments, followed by (d) connecting tubule and (e) initial collecting duct. The initial collecting ducts from several nephrons open into the collecting duct. Tubular epithelium in this lizard has similarities to that of other reptiles. The lateral borders do not overlap like in mammals, but interdigitate by fingerlike projections. The length of the nephron segments was measured in disected tubules and the diameter was measured on light and electron micrographs. From these measurements estimates of inner tubular surface area were made. Together with data from physiological studies (Stolte et al., '76; Schmidt-Nielsen, '76) the estimated surface area was used to calculate transport rates per unit area across the epithelium. Comparisons of structure and transport rates per unit area across the epithelium. Comparisons of structure and transport rates were made between S. cyanogenys and other reptiles and mammals.", "contents": "Anatomy and ultrastructure of the excretory system of the lizard, Sceloporus cyanogenys. The anatomy and ultrastructure of the lizard kidney (Sceloporus cyanogenys) have been studied by light and electron microscopy. The number of glomeruli was counted in serial sections and estimated to be 2,000 (in the two kidneys). Beginning with the glomerulus and Bowman's capsule the nephron segments are sequentially: (a) proximal tubule; (b) intermediate ciliated segment consisting of a proximal and distal part; (c) distal tubule, which can be divided into two segments, followed by (d) connecting tubule and (e) initial collecting duct. The initial collecting ducts from several nephrons open into the collecting duct. Tubular epithelium in this lizard has similarities to that of other reptiles. The lateral borders do not overlap like in mammals, but interdigitate by fingerlike projections. The length of the nephron segments was measured in disected tubules and the diameter was measured on light and electron micrographs. From these measurements estimates of inner tubular surface area were made. Together with data from physiological studies (Stolte et al., '76; Schmidt-Nielsen, '76) the estimated surface area was used to calculate transport rates per unit area across the epithelium. Comparisons of structure and transport rates per unit area across the epithelium. Comparisons of structure and transport rates were made between S. cyanogenys and other reptiles and mammals.", "PMID": 957443}
{"id": "pubmed23n0032_2723", "title": "Laser microbeam irradiation of rat kangaroo cells (PTK2) following selective sensitization with bromodeoxyuridine and ethidium bromide.", "content": "Ethidium bromide (10 mug/ml) and bromodeoxyuridine (25 mug/ml) were used to sensitize selective cell organelles to visible wavelengths of an argon ion laser (488 and 514 nanometers). Ethidium bromide was shown to be selective in sensitizing nucleoli, chromosomes, and the centriolar region of PTK2 cells to the laser microbeam. Similarly, BrDU sensitized chromosomes to the microbeam irradiation. The lesions produced on the chromosomes when either agent was used appeared as a phase paling of the irradiated segment. Nucleolar lesions also appeared as a phase paling, and the centriolar region alteration appeared either as a phase paling or a phase darkening.", "contents": "Laser microbeam irradiation of rat kangaroo cells (PTK2) following selective sensitization with bromodeoxyuridine and ethidium bromide. Ethidium bromide (10 mug/ml) and bromodeoxyuridine (25 mug/ml) were used to sensitize selective cell organelles to visible wavelengths of an argon ion laser (488 and 514 nanometers). Ethidium bromide was shown to be selective in sensitizing nucleoli, chromosomes, and the centriolar region of PTK2 cells to the laser microbeam. Similarly, BrDU sensitized chromosomes to the microbeam irradiation. The lesions produced on the chromosomes when either agent was used appeared as a phase paling of the irradiated segment. Nucleolar lesions also appeared as a phase paling, and the centriolar region alteration appeared either as a phase paling or a phase darkening.", "PMID": 957444}
{"id": "pubmed23n0032_2724", "title": "Ultrastructural investigation of the mechanism of muscle attachment to the gastropod shell.", "content": "The ultrastructure of the muscle-shell attachment was investigated in the land pulmonate snails Helix aspersa, Anguispira alternata, in the freshwater pulmonate Laevipex sp., and in the freshwater prosobranch Pomacea paludosa. In all cases, a collagenous intercellular matrix and a specialized epithelium (tendon cells) intervene between the columellar muscle and the shell. These tendon cells are characterized by hemidesmosomes at both apical and basal ends, connected by thick bundles of microfilaments. The tendon cells do not insert into the shell directly by microvilli, as formerly thought, but by an extensive network of extracellular organic fibers.", "contents": "Ultrastructural investigation of the mechanism of muscle attachment to the gastropod shell. The ultrastructure of the muscle-shell attachment was investigated in the land pulmonate snails Helix aspersa, Anguispira alternata, in the freshwater pulmonate Laevipex sp., and in the freshwater prosobranch Pomacea paludosa. In all cases, a collagenous intercellular matrix and a specialized epithelium (tendon cells) intervene between the columellar muscle and the shell. These tendon cells are characterized by hemidesmosomes at both apical and basal ends, connected by thick bundles of microfilaments. The tendon cells do not insert into the shell directly by microvilli, as formerly thought, but by an extensive network of extracellular organic fibers.", "PMID": 957445}
{"id": "pubmed23n0032_2725", "title": "The development of the septum primum relative to atrial septation in the mouse heart.", "content": "The septum primum in the mouse originates as a thickened primordium with a straight rather than a sickle-shaped ventral border. It is covered on its ventral border by anterior cushion material which is continuous over the roof of the atrium with the principal anterior cushion mass. A process of cavitation thins the septum primum and precedes actual fenestration. This process shifts the membranous septum to the left thereby providing room for the septum secundum to overlap on the right side. The septum primum cannot contact the posterior cushion until closure of the sinus venosus gutter which is described. The closure of the interatrial foramen, later the foramen primum, is accomplished by cell growth of the anterior cushion material. The ventral thick border of the septum primum contributes to the ventral limbus and the caudal thickened boundary of the fossa ovalis with some contribution from the left venous valve. These boundaries as well as the membranous portion of the interatrial septum are derived from the same primordium, namely the septum primum.", "contents": "The development of the septum primum relative to atrial septation in the mouse heart. The septum primum in the mouse originates as a thickened primordium with a straight rather than a sickle-shaped ventral border. It is covered on its ventral border by anterior cushion material which is continuous over the roof of the atrium with the principal anterior cushion mass. A process of cavitation thins the septum primum and precedes actual fenestration. This process shifts the membranous septum to the left thereby providing room for the septum secundum to overlap on the right side. The septum primum cannot contact the posterior cushion until closure of the sinus venosus gutter which is described. The closure of the interatrial foramen, later the foramen primum, is accomplished by cell growth of the anterior cushion material. The ventral thick border of the septum primum contributes to the ventral limbus and the caudal thickened boundary of the fossa ovalis with some contribution from the left venous valve. These boundaries as well as the membranous portion of the interatrial septum are derived from the same primordium, namely the septum primum.", "PMID": 957446}
{"id": "pubmed23n0032_2726", "title": "The response of transplants of cultured fetal mouse ovaries in kidneys of ovariectomized adult mice.", "content": "Whole ovaries from 16-day fetal mice were cultured for 6 to 20 days and then transplanted to the kidneys of ovariectomized adult mice where they remained for one to four weeks. After three weeks in the host's kidneys, many follicles developed within the transplants and became vesiculated. Many of the oocytes within these follicles had formed the first metaphase spindle of meiosis and several had completed the first polar body. Host mice bearing transplants that contained vesiculated follicles showed uterine stimulation and keratinization of their vaginae. However, ovaries that had been in culture for more than ten days before transplantation showed a limited response to the gonadotropins and never matured sufficiently to stimulate the host's reproductive tract. No ovulations occurred in any of the transplants.", "contents": "The response of transplants of cultured fetal mouse ovaries in kidneys of ovariectomized adult mice. Whole ovaries from 16-day fetal mice were cultured for 6 to 20 days and then transplanted to the kidneys of ovariectomized adult mice where they remained for one to four weeks. After three weeks in the host's kidneys, many follicles developed within the transplants and became vesiculated. Many of the oocytes within these follicles had formed the first metaphase spindle of meiosis and several had completed the first polar body. Host mice bearing transplants that contained vesiculated follicles showed uterine stimulation and keratinization of their vaginae. However, ovaries that had been in culture for more than ten days before transplantation showed a limited response to the gonadotropins and never matured sufficiently to stimulate the host's reproductive tract. No ovulations occurred in any of the transplants.", "PMID": 957447}
{"id": "pubmed23n0032_2727", "title": "The application of the Maatz-spreading-nail in operative treatment of distal tibial fractures.", "content": "The treatment of fractures located in the distal two fifths of the tibia offers some difficulties for anatomic and physiologic reasons. For operative treatment the conical form of the medullary cavity requires a special form of nail. Maatz developed a spreading-nail in 1943, which was applied in 107 cases between 1956 and 1974 by the present authors. Retrospective analysis of 64 cases showed advantages and limitations of this nail. If applied with proper indications very satisfactory results were seen. The operative technique described is simple.", "contents": "The application of the Maatz-spreading-nail in operative treatment of distal tibial fractures. The treatment of fractures located in the distal two fifths of the tibia offers some difficulties for anatomic and physiologic reasons. For operative treatment the conical form of the medullary cavity requires a special form of nail. Maatz developed a spreading-nail in 1943, which was applied in 107 cases between 1956 and 1974 by the present authors. Retrospective analysis of 64 cases showed advantages and limitations of this nail. If applied with proper indications very satisfactory results were seen. The operative technique described is simple.", "PMID": 957455}
{"id": "pubmed23n0032_2728", "title": "The role of thoracic aortic occlusion for massive hemoperitoneum.", "content": "Forty patients with abdominal injury and massive hemoperitoneum had left thoracotomy and thoracic aortic occlusion. All 40 patients had tense abdominal distention and 37 patients were hypotensive at the time of skin incision despite aggressive resuscitation with blood and crystalloid solution. Laparotomy was performed initially in 11 patients; seven patients had sudden cardiovascular collapse as the abdominal wall tamponade was released and four patients remained hypotensive. With thoracotomy and thoracic aortic occlusion six of the 11 patients were resuscitated and had their injuries repaired. Thoracotomy and thoracic aortic occlusion were performed before laparotomy in 29 patients: seven patients remained hypotensive and expired; blood pressure was promptly restored in 22 patients and 11 of them survived the operative procedure. Left thoracotomy and thoracic aortic occlusion, before laparotomy, is offered as an alternative approach in patients with refractory hypotension and tense, abdominal distention. This technique aids in rapid restoration of vital signs, insures continued perfusion of the brain and myocardium, provides proximal arterial control, and prevents sudden cardiac arrest as the abdominal wall tamponade is released.", "contents": "The role of thoracic aortic occlusion for massive hemoperitoneum. Forty patients with abdominal injury and massive hemoperitoneum had left thoracotomy and thoracic aortic occlusion. All 40 patients had tense abdominal distention and 37 patients were hypotensive at the time of skin incision despite aggressive resuscitation with blood and crystalloid solution. Laparotomy was performed initially in 11 patients; seven patients had sudden cardiovascular collapse as the abdominal wall tamponade was released and four patients remained hypotensive. With thoracotomy and thoracic aortic occlusion six of the 11 patients were resuscitated and had their injuries repaired. Thoracotomy and thoracic aortic occlusion were performed before laparotomy in 29 patients: seven patients remained hypotensive and expired; blood pressure was promptly restored in 22 patients and 11 of them survived the operative procedure. Left thoracotomy and thoracic aortic occlusion, before laparotomy, is offered as an alternative approach in patients with refractory hypotension and tense, abdominal distention. This technique aids in rapid restoration of vital signs, insures continued perfusion of the brain and myocardium, provides proximal arterial control, and prevents sudden cardiac arrest as the abdominal wall tamponade is released.", "PMID": 957456}
{"id": "pubmed23n0032_2729", "title": "Intraoperative autotransfusion: equipment, protocols, and guidelines.", "content": "Blood obtained by intraoperative autotransfusion is: 1) readily available 2) sterile 3) compatible 4) normothermic 5) inexpensive and may be infused rapidly for volume support. We have made extensive modifications to commercially available equipment in order to provide a safe, effective IAT. The effects of IAT in our series of 85 patients are outlined below. Red Cell Mass is reduced after IAT because of irretrievable blood loss and hemolysis, and may be controlled by homologous transfusion when necessary. Red Cell Survival is normal after IAT. Hemolysis. Plasma free hemoglobin is consistently elevated after IAT, but clears within 24 hours. Platelets are normal for patients autotransfused less than 3,500 ml; micropore filters should not be used in cases where greater than 3,500 ml blood is expected to be reinfused; in cases where greater than 3,500 ml is reinfused, 10 units of platelets are recommended for every 3,000 ml of blood reinfused; IAT does effect platelets function; however, platelets circulating within the patient function normally. Coagulation. We use local ACD to eliminate extracorporeal surface clotting. Even with massive IAT we have never demonstrated any clinical or laboratory evidence of intravascular coagulopathy. \"Dilutional coagulopathy\" may be procuced when greater than 5,000 ml are reinfused, and may be controlled with fresh frozen plasma and platelet concentrates. Bilirubin levels were normal after IAT despite gross hemoglobinuria. Fat emboli were not noted after IAT. Air emboli must be a concern in IAT; HOWEVER, PROPER OPERATION AND EQUIPMENT MODIFICATION MAY ELIMINATE EMBOLI. Renal Failure was not noted after IAT. Alveolar-arterial Oxygen Difference and Blood Gases were normal after IAT. We feel IAT is not necessary if a blood loss less than 1,000 ml is expected. Also, if greater than 3,500 ml is expected additional backup (i.e. homologous transfusions, platelets, fresh frozen plasma) may be required. As banked donor blood reserves become more limited, IAT may become a routine part of general surgical procedures.", "contents": "Intraoperative autotransfusion: equipment, protocols, and guidelines. Blood obtained by intraoperative autotransfusion is: 1) readily available 2) sterile 3) compatible 4) normothermic 5) inexpensive and may be infused rapidly for volume support. We have made extensive modifications to commercially available equipment in order to provide a safe, effective IAT. The effects of IAT in our series of 85 patients are outlined below. Red Cell Mass is reduced after IAT because of irretrievable blood loss and hemolysis, and may be controlled by homologous transfusion when necessary. Red Cell Survival is normal after IAT. Hemolysis. Plasma free hemoglobin is consistently elevated after IAT, but clears within 24 hours. Platelets are normal for patients autotransfused less than 3,500 ml; micropore filters should not be used in cases where greater than 3,500 ml blood is expected to be reinfused; in cases where greater than 3,500 ml is reinfused, 10 units of platelets are recommended for every 3,000 ml of blood reinfused; IAT does effect platelets function; however, platelets circulating within the patient function normally. Coagulation. We use local ACD to eliminate extracorporeal surface clotting. Even with massive IAT we have never demonstrated any clinical or laboratory evidence of intravascular coagulopathy. \"Dilutional coagulopathy\" may be procuced when greater than 5,000 ml are reinfused, and may be controlled with fresh frozen plasma and platelet concentrates. Bilirubin levels were normal after IAT despite gross hemoglobinuria. Fat emboli were not noted after IAT. Air emboli must be a concern in IAT; HOWEVER, PROPER OPERATION AND EQUIPMENT MODIFICATION MAY ELIMINATE EMBOLI. Renal Failure was not noted after IAT. Alveolar-arterial Oxygen Difference and Blood Gases were normal after IAT. We feel IAT is not necessary if a blood loss less than 1,000 ml is expected. Also, if greater than 3,500 ml is expected additional backup (i.e. homologous transfusions, platelets, fresh frozen plasma) may be required. As banked donor blood reserves become more limited, IAT may become a routine part of general surgical procedures.", "PMID": 957457}
{"id": "pubmed23n0032_2730", "title": "Mandibular fractures: the problem patient.", "content": "Of 110 adult patients with 163 mandibular fractures treated at University of Pennsylvania Graduate Hospital from 1964 to 1974, 17% had a history of previous mandibular fractures. All patients' medical records and X-rays were reviewed. Because of the characteristics of this patient population, it seems well suited for discussion as a problem group. Mandibular fractures in this urban area are most commonly caused by blows of a fist and the patient is often intoxicated, which impedes diagnosis, delays treatment, and probably results in further complications from injuries. Patients frequently do not comply with treatment recommendations, do discontinue immobilization, and fail to return for followup, even for removal of intermaxillary fixation. Almost one third had insufficient teeth for fixation; many had poor oral hygiene, and teeth in the fracture line, which led to complications. Osteomyelitis resulting in nonunion and requiring bone grafting occurred in seven patients. Recommended are: simplest fixation methods, minimal amounts of surgery, and as few extractions as possible. Experience from this patient group may be of help whenever a problem patient is seen with a mandibular fracture.", "contents": "Mandibular fractures: the problem patient. Of 110 adult patients with 163 mandibular fractures treated at University of Pennsylvania Graduate Hospital from 1964 to 1974, 17% had a history of previous mandibular fractures. All patients' medical records and X-rays were reviewed. Because of the characteristics of this patient population, it seems well suited for discussion as a problem group. Mandibular fractures in this urban area are most commonly caused by blows of a fist and the patient is often intoxicated, which impedes diagnosis, delays treatment, and probably results in further complications from injuries. Patients frequently do not comply with treatment recommendations, do discontinue immobilization, and fail to return for followup, even for removal of intermaxillary fixation. Almost one third had insufficient teeth for fixation; many had poor oral hygiene, and teeth in the fracture line, which led to complications. Osteomyelitis resulting in nonunion and requiring bone grafting occurred in seven patients. Recommended are: simplest fixation methods, minimal amounts of surgery, and as few extractions as possible. Experience from this patient group may be of help whenever a problem patient is seen with a mandibular fracture.", "PMID": 957459}
{"id": "pubmed23n0032_2731", "title": "Vertical fracture dislocation of the tarsal navicular.", "content": "An isolated vertical fracture dislocation of the left tarsal navicular with some comminution was successfully treated with open reduction and internal fixation with a screw. The surgical treatment is described, and early mobilization strongly recommended.", "contents": "Vertical fracture dislocation of the tarsal navicular. An isolated vertical fracture dislocation of the left tarsal navicular with some comminution was successfully treated with open reduction and internal fixation with a screw. The surgical treatment is described, and early mobilization strongly recommended.", "PMID": 957460}
{"id": "pubmed23n0032_2732", "title": "Volar fracture dislocations of the second and third carpometacarpal joints associated with acute carpal tunnel syndrome.", "content": "Volar fracture dislocation of the second and third metacarpal bases associated with acute carpal tunnel syndrome in a 17-year-old football player were treated with open reduction, via volar incision to decompress the carpal tunnel, and via a second dorsal incision for internal fixation of the fracture dislocations with Kirschner wire fixation and reinsertion of the avulsed extensor carpi radialis:longus tendon. Anatomic reduction by closed or open reduction is recommended to avoid leaving patients with a weak grip and pain over the site of the fracture.", "contents": "Volar fracture dislocations of the second and third carpometacarpal joints associated with acute carpal tunnel syndrome. Volar fracture dislocation of the second and third metacarpal bases associated with acute carpal tunnel syndrome in a 17-year-old football player were treated with open reduction, via volar incision to decompress the carpal tunnel, and via a second dorsal incision for internal fixation of the fracture dislocations with Kirschner wire fixation and reinsertion of the avulsed extensor carpi radialis:longus tendon. Anatomic reduction by closed or open reduction is recommended to avoid leaving patients with a weak grip and pain over the site of the fracture.", "PMID": 957461}
{"id": "pubmed23n0032_2733", "title": "Infection with Schistosoma mansoni in the Gezire area of the Sudan.", "content": "A survey for schistosomiasis in a village in the Gezira area of the Sudan in 1973 showed that there has probably been a great increase in the incidence of S. mansoni infection in the last 25 years. Severe morbidity was uncommon in this sample but overt infection was associated with the symptoms of bloody diarrhoea, tiredness, and possibly abdominal pain, and with a reduced level of haemoglobin. Hepatosplenomegaly was common and schistosomiasis is considered to have contributed to this, although hyperendemic malaria must also have been important in its causation.", "contents": "Infection with Schistosoma mansoni in the Gezire area of the Sudan. A survey for schistosomiasis in a village in the Gezira area of the Sudan in 1973 showed that there has probably been a great increase in the incidence of S. mansoni infection in the last 25 years. Severe morbidity was uncommon in this sample but overt infection was associated with the symptoms of bloody diarrhoea, tiredness, and possibly abdominal pain, and with a reduced level of haemoglobin. Hepatosplenomegaly was common and schistosomiasis is considered to have contributed to this, although hyperendemic malaria must also have been important in its causation.", "PMID": 957463}
{"id": "pubmed23n0032_2734", "title": "Patterns of acute renal failure in leptospirosis.", "content": "The renal profile was studied in 61 patients having leptospirosis. Leptospira-induced acute renal failure conformed to one of two distinct clinical patterns, either oliguric on non-oliguric renal failure. The prognosis was excellent in the latter variety of renal failure and mortality was confined to patients with oliguric renal failure, where severe glomerular and widespread tubulo-interstitial lesions were invariably present. These patients had unfavourable prognostic features during life, such as prolonged oliguria and anuria, absence of a diruetic phase, persistent elevations in BUN and persistently low urea excretion. The prognostic significance of the age of the patients, hypotension and jaundice in relation to the occurrence and type of acute renal failure have been discussed. Retrospective observations indicate that both a vasculotoxic or haemorrhagic state and oliguric acute renal failure are important causes for mortality in human leptospiral infections.", "contents": "Patterns of acute renal failure in leptospirosis. The renal profile was studied in 61 patients having leptospirosis. Leptospira-induced acute renal failure conformed to one of two distinct clinical patterns, either oliguric on non-oliguric renal failure. The prognosis was excellent in the latter variety of renal failure and mortality was confined to patients with oliguric renal failure, where severe glomerular and widespread tubulo-interstitial lesions were invariably present. These patients had unfavourable prognostic features during life, such as prolonged oliguria and anuria, absence of a diruetic phase, persistent elevations in BUN and persistently low urea excretion. The prognostic significance of the age of the patients, hypotension and jaundice in relation to the occurrence and type of acute renal failure have been discussed. Retrospective observations indicate that both a vasculotoxic or haemorrhagic state and oliguric acute renal failure are important causes for mortality in human leptospiral infections.", "PMID": 957464}
{"id": "pubmed23n0032_2735", "title": "Immunological response to tetanus toxoid during a schistosomal infection in mice.", "content": "Mice infected with Schistosoma mansoni are unable to mount the same antibody response to tetanus toxoid as uninfected mice; the reduction in response does not occur during the prepatent period.", "contents": "Immunological response to tetanus toxoid during a schistosomal infection in mice. Mice infected with Schistosoma mansoni are unable to mount the same antibody response to tetanus toxoid as uninfected mice; the reduction in response does not occur during the prepatent period.", "PMID": 957465}
{"id": "pubmed23n0032_2736", "title": "Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.", "content": "A DNA protein complex that is a precursor of mature phi X174 phage was isolated. The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA. The viral-strand DNA ranged from one to two genomes in length. Proteins coded on the phi X174 genome as well as the host genome were associated with the viral DNA in the 50S precursor complex. Our results indicated that both viral DNA synthesis and cleavage of the growing viral-strand DNA occurred in the 50S complex.", "contents": "Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174. A DNA protein complex that is a precursor of mature phi X174 phage was isolated. The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA. The viral-strand DNA ranged from one to two genomes in length. Proteins coded on the phi X174 genome as well as the host genome were associated with the viral DNA in the 50S precursor complex. Our results indicated that both viral DNA synthesis and cleavage of the growing viral-strand DNA occurred in the 50S complex.", "PMID": 957476}
{"id": "pubmed23n0032_2737", "title": "Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo.", "content": "A functional gene A product of phi X174 was found to be required at the stage of single-stranded DNA synthesis. A precursor complex that synthesizes single-stranded DNA (50S complex [Fujisawa and Hayashi, 1976]) was isolated from cells infected with wild-type or with temperature-sensitive gene A mutant phage. Proper cleavage of the single-stranded viral DNA did not occur in cells infected with the temperature-sensitive gene A mutant under restrictive conditions. This resulted in (i) accumulation of linear viral DNA molecules of 2 units in length in the 50S complex and (ii) cessation of elongation of viral-strand DNA after one complete cycle of single-stranded DNA synthesis.", "contents": "Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo. A functional gene A product of phi X174 was found to be required at the stage of single-stranded DNA synthesis. A precursor complex that synthesizes single-stranded DNA (50S complex [Fujisawa and Hayashi, 1976]) was isolated from cells infected with wild-type or with temperature-sensitive gene A mutant phage. Proper cleavage of the single-stranded viral DNA did not occur in cells infected with the temperature-sensitive gene A mutant under restrictive conditions. This resulted in (i) accumulation of linear viral DNA molecules of 2 units in length in the 50S complex and (ii) cessation of elongation of viral-strand DNA after one complete cycle of single-stranded DNA synthesis.", "PMID": 957477}
{"id": "pubmed23n0032_2738", "title": "Nature of the molecular heterogeneity of human leukocyte interferon.", "content": "The existence of two components of human leukocyte interferon has been recently reported. In the present study, the nature of this molecular heterogeneity was explored by affinity chromatography on immobilized micro- and macroligands, ion-exchange chromatography, and molecular sieving. Chromatography on a series of alkyl-agarose adsorbents shows, for the first time, the intrinsic hydrophobicity of human leukocyte interferon. Additionally, the separation of two interferon components is achieved by use of the alkyl-agarose as well as by the omega-aminoalkyl-agarose adsorbents. Clear-cut separation of the two components was also achieved by chromatography on BSA-CH-Sepharose and on DEAE-Bio Gel A. An important feature of these separations is that they do not require the use of denaturing conditions. The molecular weights of the leukocyte interferon components, as determined on Sephadex G-75, are quite similar or identical, approximately 26,000. Thus, the molecular heterogeneity of human leukocyte interferon can be attributed, at least in part, to differences in the hydrophobicity and ionic properties of its two components.", "contents": "Nature of the molecular heterogeneity of human leukocyte interferon. The existence of two components of human leukocyte interferon has been recently reported. In the present study, the nature of this molecular heterogeneity was explored by affinity chromatography on immobilized micro- and macroligands, ion-exchange chromatography, and molecular sieving. Chromatography on a series of alkyl-agarose adsorbents shows, for the first time, the intrinsic hydrophobicity of human leukocyte interferon. Additionally, the separation of two interferon components is achieved by use of the alkyl-agarose as well as by the omega-aminoalkyl-agarose adsorbents. Clear-cut separation of the two components was also achieved by chromatography on BSA-CH-Sepharose and on DEAE-Bio Gel A. An important feature of these separations is that they do not require the use of denaturing conditions. The molecular weights of the leukocyte interferon components, as determined on Sephadex G-75, are quite similar or identical, approximately 26,000. Thus, the molecular heterogeneity of human leukocyte interferon can be attributed, at least in part, to differences in the hydrophobicity and ionic properties of its two components.", "PMID": 957478}
{"id": "pubmed23n0032_2739", "title": "Phospholipid metabolism in Pseudomonas BAL-31 infected with lipid-containing bacteriophage PM2.", "content": "Infection of Pseudomonas BAL-31 with the lipid-containing bacteriophage PM2 resulted in no detectable change in the rate of phosphatidylglycerol (PG) or phosphatidylethanolamine (PE) biosynthesis. An increase in the PG content of infected cultures was not seen until the cultures began to lyse, and this increase was in fact only a relative increase resulting from the extensive turnover of PE at the onset of culture lysis. Turnover studies revealed that the glycerol, phosphorus fatty acid, and ethanolamine moieties of PE turned over simultaneously at the time of lysis, and therefore made it unlikely that there was a PE to PG conversion during the latent period of the phage. The lipid found in the bacteriophage did not reflect a preferential selection for lipid synthesized before or after infection, but in fact reflected the composition of the host membrane at the time the phage were assembled. The use of a modified medium that allowed the cultivation of Pseudomonas BAL-31 as a prototroph and resulted in reliable lysis times of infected cultures led us to the conclusion that PM2 infection effects little change in host phospholipid metabolism, and that there is sufficient PG in the host cytoplasmic membrane to account for a full burst of phage. As a result of the reliable lysis times that we have achieved, we concluded that certain metabolic events, i.e., PE turnover, are lytic phenomena and must not be confused with events relevant to the biosynthesis and maturation of the phage.", "contents": "Phospholipid metabolism in Pseudomonas BAL-31 infected with lipid-containing bacteriophage PM2. Infection of Pseudomonas BAL-31 with the lipid-containing bacteriophage PM2 resulted in no detectable change in the rate of phosphatidylglycerol (PG) or phosphatidylethanolamine (PE) biosynthesis. An increase in the PG content of infected cultures was not seen until the cultures began to lyse, and this increase was in fact only a relative increase resulting from the extensive turnover of PE at the onset of culture lysis. Turnover studies revealed that the glycerol, phosphorus fatty acid, and ethanolamine moieties of PE turned over simultaneously at the time of lysis, and therefore made it unlikely that there was a PE to PG conversion during the latent period of the phage. The lipid found in the bacteriophage did not reflect a preferential selection for lipid synthesized before or after infection, but in fact reflected the composition of the host membrane at the time the phage were assembled. The use of a modified medium that allowed the cultivation of Pseudomonas BAL-31 as a prototroph and resulted in reliable lysis times of infected cultures led us to the conclusion that PM2 infection effects little change in host phospholipid metabolism, and that there is sufficient PG in the host cytoplasmic membrane to account for a full burst of phage. As a result of the reliable lysis times that we have achieved, we concluded that certain metabolic events, i.e., PE turnover, are lytic phenomena and must not be confused with events relevant to the biosynthesis and maturation of the phage.", "PMID": 957479}
{"id": "pubmed23n0032_2740", "title": "Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein).", "content": "Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates.", "contents": "Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein). Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates.", "PMID": 957480}
{"id": "pubmed23n0032_2741", "title": "Intermediates in adenovirus assembly.", "content": "Three intermediates in adenovirus assembly have been defined; nuclear intermediates, young virions, and mature virions. The nuclear intermediates are fragile and heterogenous in size (550S-670S) and withstand separation on ficoll gradients but fall apart upon CsCl gradient centrifugation unless prefixed with glutaraldehyde. They contain both capsid and core structures, and the core structures are preferentially released during purification in CsCl. The precursor polypeptides pVI and pVII are present in the intermediates without any corresponding mature polypeptide. The young virions (Ishibashi and Maizel, 1974) are stable and preferentially confined to the nuclei after cell fractionation. They contain both uncleaved precursor polypeptides and their cleavage products. The mature virions accumulate in the cytoplasm during cell fractionation and contain the final mature polypeptides. Pulse-chase labeling kinetics, focusing on the precursor polypeptides, suggest that these three classes participate in assembly of adenovirus. Tryptic peptide maps establish that polypeptide pVI is the precursor of polypeptide VI, but only a small fraction of polypeptide 26K can in vivo account for polypeptide VIII.", "contents": "Intermediates in adenovirus assembly. Three intermediates in adenovirus assembly have been defined; nuclear intermediates, young virions, and mature virions. The nuclear intermediates are fragile and heterogenous in size (550S-670S) and withstand separation on ficoll gradients but fall apart upon CsCl gradient centrifugation unless prefixed with glutaraldehyde. They contain both capsid and core structures, and the core structures are preferentially released during purification in CsCl. The precursor polypeptides pVI and pVII are present in the intermediates without any corresponding mature polypeptide. The young virions (Ishibashi and Maizel, 1974) are stable and preferentially confined to the nuclei after cell fractionation. They contain both uncleaved precursor polypeptides and their cleavage products. The mature virions accumulate in the cytoplasm during cell fractionation and contain the final mature polypeptides. Pulse-chase labeling kinetics, focusing on the precursor polypeptides, suggest that these three classes participate in assembly of adenovirus. Tryptic peptide maps establish that polypeptide pVI is the precursor of polypeptide VI, but only a small fraction of polypeptide 26K can in vivo account for polypeptide VIII.", "PMID": 957481}
{"id": "pubmed23n0032_2742", "title": "Defective bud formation in human cells chronically infected with subacute sclerosing panencephalitis virus.", "content": "Human prostate cells chronically infected with the Mantooth strain of subacute sclerosing panencephalitis (SSPE) virus multiply normally, fuse only occasionally to form giant cells, and yet have twisted intracytoplasmic nucleocapsids. These cells are able to support replication of vesicular stomatitis virus, although they release only small amounts of SSPE virus. To determine why carrier cells do not produce virus, they were examined with techniques for surface replication, freeze-fracturing, and immunoperoxidase labeling with SSPE antibody. The surface of carrier cells, like that of productive cells, is characterized by ridges crowned with viral antigens and devoid of the intramembrane particles revealed by freeze-fracture techniques. Since surface ridges form where nucleocapsids attach to the membrane, the shape and length of ridges are indicative of the shape and length of the underlying nucleocapsid. Whereas ridges on productive cells are serpentine in shape, those on carrier cells are typically straight or hairpin shaped, and the hairpin ridges are twice as long as serpentine ridges on productive cells. Furthermore, the spacing between ridges on carrier cells is never as small as that in productive infections, so that continuous sheets of viral membrane are never formed. The majority of carrier cells lack the round viral buds observed in productive cells but have, instead, many elongated processes attached to the cell surface. Each of these processes contains one or two hairpin ridges overlying hairpin-shaped nucleocapsids. These \"hairpin buds\" are restricted to a single region of the carrier cell surface, whereas viral buds are distributed over the entire surface of productive cells. Thus, there are several structural defects in carrier cells that depend on the specific interaction of a certain viral strain with a certain cell type. These defects prevent the deployment of viral antigen in some regions of the cell surface, the formation of nucleocapsids of normal length, the coiling of attached nucleocapsids, and the consolidation of sheets of viral membrane into spherical buds with the nucleocapsids coiled inside. These defects may account for the failure of carrier cells to shed infectious virus.", "contents": "Defective bud formation in human cells chronically infected with subacute sclerosing panencephalitis virus. Human prostate cells chronically infected with the Mantooth strain of subacute sclerosing panencephalitis (SSPE) virus multiply normally, fuse only occasionally to form giant cells, and yet have twisted intracytoplasmic nucleocapsids. These cells are able to support replication of vesicular stomatitis virus, although they release only small amounts of SSPE virus. To determine why carrier cells do not produce virus, they were examined with techniques for surface replication, freeze-fracturing, and immunoperoxidase labeling with SSPE antibody. The surface of carrier cells, like that of productive cells, is characterized by ridges crowned with viral antigens and devoid of the intramembrane particles revealed by freeze-fracture techniques. Since surface ridges form where nucleocapsids attach to the membrane, the shape and length of ridges are indicative of the shape and length of the underlying nucleocapsid. Whereas ridges on productive cells are serpentine in shape, those on carrier cells are typically straight or hairpin shaped, and the hairpin ridges are twice as long as serpentine ridges on productive cells. Furthermore, the spacing between ridges on carrier cells is never as small as that in productive infections, so that continuous sheets of viral membrane are never formed. The majority of carrier cells lack the round viral buds observed in productive cells but have, instead, many elongated processes attached to the cell surface. Each of these processes contains one or two hairpin ridges overlying hairpin-shaped nucleocapsids. These \"hairpin buds\" are restricted to a single region of the carrier cell surface, whereas viral buds are distributed over the entire surface of productive cells. Thus, there are several structural defects in carrier cells that depend on the specific interaction of a certain viral strain with a certain cell type. These defects prevent the deployment of viral antigen in some regions of the cell surface, the formation of nucleocapsids of normal length, the coiling of attached nucleocapsids, and the consolidation of sheets of viral membrane into spherical buds with the nucleocapsids coiled inside. These defects may account for the failure of carrier cells to shed infectious virus.", "PMID": 957482}
{"id": "pubmed23n0032_2743", "title": "7-Methyl-guanosine and efficiency of RNA translation.", "content": "Brome mosaic virus RNAs 3 and 4 were chemically modified to remove the terminal 7-methyl-guanosine (m7G) structure, and the modified RNAs were tested for their messenger activity in a cell-free system derived from wheat embryo. Amino acid incorporation and ribosome-binding data show that removal of m7G results in reduction, but not complete abolition, of the messenger activity of the RNA. This suggests that the function of m7G may be related to efficient translation of messenger RNA. Possible involvement of other structural factors in RNA translation is discussed.", "contents": "7-Methyl-guanosine and efficiency of RNA translation. Brome mosaic virus RNAs 3 and 4 were chemically modified to remove the terminal 7-methyl-guanosine (m7G) structure, and the modified RNAs were tested for their messenger activity in a cell-free system derived from wheat embryo. Amino acid incorporation and ribosome-binding data show that removal of m7G results in reduction, but not complete abolition, of the messenger activity of the RNA. This suggests that the function of m7G may be related to efficient translation of messenger RNA. Possible involvement of other structural factors in RNA translation is discussed.", "PMID": 957483}
{"id": "pubmed23n0032_2744", "title": "Characterization of a temperature-sensitive, hexon transport mutant of type 5 adenovirus.", "content": "Infection of KB cells at 39.5 degrees C with H5ts147, a temperature-sensitive (ts) mutant of type 5 adenovirus, resulted in the cytoplasmic accumulation of hexon antigen; all other virion proteins measured, however, were normally transported into the nucleus. Immunofluorescence techniques were used to study the intracellular location of viral proteins. Genetic studies revealed that H5ts147 was the single member of a nonoverlapping complementation group and occupied a unique locus on the adenovirus genetic map, distinct from mutants that failed to produce immunologically reactive hexons at 39.5 degrees C (\"hexon-minus\" mutants). Sedimentation studies of extracts of H5ts147-infected cells cultured and labeled at 39.5 degrees C revealed the production of 12S hexon capsomers (the native, trimeric structures), which were immunoprecipitable to the same extent as hexons synthesized in wild type (WT)-infected cells. In contrast, only 3.4S polypeptide chains were found in extracts of cells infected with the class of mutants unable to produce immunologically reactive hexon protein at 39.5 degrees C. Hexons synthesized in H5ts147-infected cells at 39.5 degrees C were capable of being assembled into virions, to the same extent as hexons synthesized in WT-infected cells, when the temperature was shifted down to the permissive temperature, 32 degrees C. Infectious virus production was initiated within 2 to 6 h after shift-down to 32 degrees C; de novo protein synthesis was required to allow this increase in viral titer. If ts147-infected cells were shifted up to 39.5 degrees C late in the viral multiplication cycle, viral production was arrested within 1 to 2 h. The kinetics of shutoff was similar to that of a WT-infected culture treated with cycloheximide at the time of shift-up. The P-VI nonvirion polypeptide, the precursor to virion protein VI, was unstable at 39.5 degrees C, whereas the hexon polypeptide was not degraded during the chase. It appears that there is a structural requirement for the transport of hexons into the nucleus more stringent than the acquisition of immunological reactivity and folding into the 12S form.", "contents": "Characterization of a temperature-sensitive, hexon transport mutant of type 5 adenovirus. Infection of KB cells at 39.5 degrees C with H5ts147, a temperature-sensitive (ts) mutant of type 5 adenovirus, resulted in the cytoplasmic accumulation of hexon antigen; all other virion proteins measured, however, were normally transported into the nucleus. Immunofluorescence techniques were used to study the intracellular location of viral proteins. Genetic studies revealed that H5ts147 was the single member of a nonoverlapping complementation group and occupied a unique locus on the adenovirus genetic map, distinct from mutants that failed to produce immunologically reactive hexons at 39.5 degrees C (\"hexon-minus\" mutants). Sedimentation studies of extracts of H5ts147-infected cells cultured and labeled at 39.5 degrees C revealed the production of 12S hexon capsomers (the native, trimeric structures), which were immunoprecipitable to the same extent as hexons synthesized in wild type (WT)-infected cells. In contrast, only 3.4S polypeptide chains were found in extracts of cells infected with the class of mutants unable to produce immunologically reactive hexon protein at 39.5 degrees C. Hexons synthesized in H5ts147-infected cells at 39.5 degrees C were capable of being assembled into virions, to the same extent as hexons synthesized in WT-infected cells, when the temperature was shifted down to the permissive temperature, 32 degrees C. Infectious virus production was initiated within 2 to 6 h after shift-down to 32 degrees C; de novo protein synthesis was required to allow this increase in viral titer. If ts147-infected cells were shifted up to 39.5 degrees C late in the viral multiplication cycle, viral production was arrested within 1 to 2 h. The kinetics of shutoff was similar to that of a WT-infected culture treated with cycloheximide at the time of shift-up. The P-VI nonvirion polypeptide, the precursor to virion protein VI, was unstable at 39.5 degrees C, whereas the hexon polypeptide was not degraded during the chase. It appears that there is a structural requirement for the transport of hexons into the nucleus more stringent than the acquisition of immunological reactivity and folding into the 12S form.", "PMID": 957484}
{"id": "pubmed23n0032_2745", "title": "Utilization of early promotors in mutant far P85 of bacteriophage T4.", "content": "We show that farP85 is a recessive mutant of T4 incapable of activating the delayed early promotors for genes 43 and 45 and that the farP85 mutation is in the same complementation group as the ts G1 mutation, which is located in the \"modifier of transcription\" (mot) gene.", "contents": "Utilization of early promotors in mutant far P85 of bacteriophage T4. We show that farP85 is a recessive mutant of T4 incapable of activating the delayed early promotors for genes 43 and 45 and that the farP85 mutation is in the same complementation group as the ts G1 mutation, which is located in the \"modifier of transcription\" (mot) gene.", "PMID": 957485}
{"id": "pubmed23n0032_2746", "title": "Genome structure of incomplete particles of adenovirus.", "content": "Incomplete particles arising during productive growth of adenovirus were separated from infectious particles by density gradient centrifugation. The DNA contained in particles of low density was characterized by restriction enzyme analysis and by electron microscopy and heteroduplexing techniques. The DNA is heterogeneous in length, ranging in size from 15% of the normal genome to full length. Many individual molecules contain long, inverted terminal repetitions, which consist of the sequences extending from the normal left-hand end of the viral genome inward; the normal right end sequences appear to be missing from these molecules. The region of the genome reiterated in these molecules is that which has been implicated in transformation of rat cells by adenovirus (Gallimore, Sharp, and Sambrook, 1974; Graham, van der Eb, and Heijneker, 1974). A model for adenovirus replication is presented that accounts for the aberrant structures observed.", "contents": "Genome structure of incomplete particles of adenovirus. Incomplete particles arising during productive growth of adenovirus were separated from infectious particles by density gradient centrifugation. The DNA contained in particles of low density was characterized by restriction enzyme analysis and by electron microscopy and heteroduplexing techniques. The DNA is heterogeneous in length, ranging in size from 15% of the normal genome to full length. Many individual molecules contain long, inverted terminal repetitions, which consist of the sequences extending from the normal left-hand end of the viral genome inward; the normal right end sequences appear to be missing from these molecules. The region of the genome reiterated in these molecules is that which has been implicated in transformation of rat cells by adenovirus (Gallimore, Sharp, and Sambrook, 1974; Graham, van der Eb, and Heijneker, 1974). A model for adenovirus replication is presented that accounts for the aberrant structures observed.", "PMID": 957486}
{"id": "pubmed23n0032_2747", "title": "Radiculitis distress as a mimic of renal pain.", "content": "It is the experience of the urological author that radiculitis secondary to costovertebral joint derangement is the most common cause of lower abdominal pain. However, this pain is sometimes made worse when the patient is subjected to a flank incision for presumed renal disease, since the aftermath of a flank incision may be a downward pull on a rib owing to detachments of muscles attached to its superior surface. Emotional problems, too, befall many patients with radiculitis--despondency over delayed diagnoses or sensitivity at having been told their complaints are psychosomatic. Most often these difficulties disappear spontaneously once the pain is relieved. Definitive diagnosis requires orthopedic techniques. Unfortunately, few orthopedists are well versed or interested in the syndrome of renal pain. When they are, erroneous diagnosis can be corrected and a course of conservative or surgical treatment prescribed, with excellent results.", "contents": "Radiculitis distress as a mimic of renal pain. It is the experience of the urological author that radiculitis secondary to costovertebral joint derangement is the most common cause of lower abdominal pain. However, this pain is sometimes made worse when the patient is subjected to a flank incision for presumed renal disease, since the aftermath of a flank incision may be a downward pull on a rib owing to detachments of muscles attached to its superior surface. Emotional problems, too, befall many patients with radiculitis--despondency over delayed diagnoses or sensitivity at having been told their complaints are psychosomatic. Most often these difficulties disappear spontaneously once the pain is relieved. Definitive diagnosis requires orthopedic techniques. Unfortunately, few orthopedists are well versed or interested in the syndrome of renal pain. When they are, erroneous diagnosis can be corrected and a course of conservative or surgical treatment prescribed, with excellent results.", "PMID": 957487}
{"id": "pubmed23n0032_2748", "title": "Evidence for a new mammalian organ: a preliminary report.", "content": "During an investigation on the mechanisms by which mannitol increases renal blood flow, using an in vitro perfused rat kidney preparation, it was observed that an osmotic challenge (320 mOsm) was followed by the accumulation of chloride and calcium ions in the renal medulla. Although the chloride was derived from the perfusate, the source of calcium appeared to be localized in the renal sinus fatty tissue. A morphologic study of this area reveals the existence of a tissue complex, having the characteristics of a hitherto unrecognized organ or gland communicating with the renal parenchyma via a portal vascular system. The possible physiologic significance of this complex is discussed.", "contents": "Evidence for a new mammalian organ: a preliminary report. During an investigation on the mechanisms by which mannitol increases renal blood flow, using an in vitro perfused rat kidney preparation, it was observed that an osmotic challenge (320 mOsm) was followed by the accumulation of chloride and calcium ions in the renal medulla. Although the chloride was derived from the perfusate, the source of calcium appeared to be localized in the renal sinus fatty tissue. A morphologic study of this area reveals the existence of a tissue complex, having the characteristics of a hitherto unrecognized organ or gland communicating with the renal parenchyma via a portal vascular system. The possible physiologic significance of this complex is discussed.", "PMID": 957488}
{"id": "pubmed23n0032_2749", "title": "The use of tetrazolium to determine hydronephrotic damage in human kidneys.", "content": "A study detailing an assay for evaluating hydronephrotic damage is presented. We used nitroblue tetrazolium, which is reduced by intact renal tissue. When marked hydronephrotic damage has occurred little evidence of tetrazolium reducing activity is present. The clinical application has been helpful in determining the extent of damage and the subsequent need for removal of the kidney in question. When the test indicated functioning renal tissue a repair of the obstruction was performed with subsequent improvement in function.", "contents": "The use of tetrazolium to determine hydronephrotic damage in human kidneys. A study detailing an assay for evaluating hydronephrotic damage is presented. We used nitroblue tetrazolium, which is reduced by intact renal tissue. When marked hydronephrotic damage has occurred little evidence of tetrazolium reducing activity is present. The clinical application has been helpful in determining the extent of damage and the subsequent need for removal of the kidney in question. When the test indicated functioning renal tissue a repair of the obstruction was performed with subsequent improvement in function.", "PMID": 957489}
{"id": "pubmed23n0032_2750", "title": "Experiences with 16 cases of pararenal pseudocyst.", "content": "Pararenal pseudocysts developed after an operation, after blunt and penetrating trauma or after contiguous disease processes in 16 patients. Nephrectomy was necessary in 8 cases. Although the duration of the pseudocyst and the extent of conduit injury did to an extent determine fate of the kidney, the location of the encapsulated extravasate and its propensity for curtailment of urine formation appeared to be more significant factors. The cases were reviewed in light of the individualization in management required for the complex problems they present.", "contents": "Experiences with 16 cases of pararenal pseudocyst. Pararenal pseudocysts developed after an operation, after blunt and penetrating trauma or after contiguous disease processes in 16 patients. Nephrectomy was necessary in 8 cases. Although the duration of the pseudocyst and the extent of conduit injury did to an extent determine fate of the kidney, the location of the encapsulated extravasate and its propensity for curtailment of urine formation appeared to be more significant factors. The cases were reviewed in light of the individualization in management required for the complex problems they present.", "PMID": 957490}
{"id": "pubmed23n0032_2751", "title": "Sonography of the Page kidney.", "content": "We used sonography to investigate 3 patients with hypertension owing to a Page kidney phenomenon. In 2 cases a perinephric cystic collection was compressing the kidney, while in 1 case there was a large perinephric organized hematoma. When a Page kidney is suspected in a hypertensive patient sonography is a reasonable next step in the evaluation, after the excretory urogram, and may obviate the need for angiography.", "contents": "Sonography of the Page kidney. We used sonography to investigate 3 patients with hypertension owing to a Page kidney phenomenon. In 2 cases a perinephric cystic collection was compressing the kidney, while in 1 case there was a large perinephric organized hematoma. When a Page kidney is suspected in a hypertensive patient sonography is a reasonable next step in the evaluation, after the excretory urogram, and may obviate the need for angiography.", "PMID": 957491}
{"id": "pubmed23n0032_2752", "title": "Infusion pyelography in anuric patients.", "content": "The spectrum for the use of retrograde pyelography in renal failure has been narrowed further by extending the facility of infusion urography to anuric patients. The latter study has helped greatly in sifting apart pre-renal and renal anuria patients from those with obstructive uropathy. In obstructive anuria infusion urography has proved comprehensive in influencing judgment about the exact site and nature of obstruction, in affixing laterality towards the better functioning renal unit and in deciding a suitable definitive operation or planned staged procedures.", "contents": "Infusion pyelography in anuric patients. The spectrum for the use of retrograde pyelography in renal failure has been narrowed further by extending the facility of infusion urography to anuric patients. The latter study has helped greatly in sifting apart pre-renal and renal anuria patients from those with obstructive uropathy. In obstructive anuria infusion urography has proved comprehensive in influencing judgment about the exact site and nature of obstruction, in affixing laterality towards the better functioning renal unit and in deciding a suitable definitive operation or planned staged procedures.", "PMID": 957492}
{"id": "pubmed23n0032_2753", "title": "Peripelvic extravasation, urinoma formation and tumor obstruction of the ureter.", "content": "We report 4 cases of peripelvic extravasation caused by tumor obstruction of the ureter. A urinoma developed in 3 of the 4 cases. Review of the literature reveals only 5 previously reported cases of peripelvic extravasation secondary to tumor obstruction of the urinary tract. We discuss the mechanics of peripelvic extravasation and urinoma formation, and conclude that tumor obstruction of the ureter must be considered in all cases of peripelvic extravasation.", "contents": "Peripelvic extravasation, urinoma formation and tumor obstruction of the ureter. We report 4 cases of peripelvic extravasation caused by tumor obstruction of the ureter. A urinoma developed in 3 of the 4 cases. Review of the literature reveals only 5 previously reported cases of peripelvic extravasation secondary to tumor obstruction of the urinary tract. We discuss the mechanics of peripelvic extravasation and urinoma formation, and conclude that tumor obstruction of the ureter must be considered in all cases of peripelvic extravasation.", "PMID": 957493}
{"id": "pubmed23n0032_2754", "title": "Six years of experience with retrograde biopsy of intraureteral carcinoma using the Dormia stone basket.", "content": "To confirm the cause of a small defect of the ureter and to determine preoperatively the histological grade of ureteral carcinoma a new technique of intraureteral biopsy was attempted with a Dormia stone basket. Of 8 biopsies performed on 7 patients with ureteral or pelvioureteral carcinoma only 1 failed to produce a fragment of tissue. Histological diagnosis was made in each of the 7 patients and histological grading in 5. Four patients with non-malignant defects of the lower ureter also were biopsied with this technique to ascertain the feasibility of this biopsy on minimal filling defects. In each patient a tiny piece of tissue was removed. Histological diagnosis could be made in 3 cases.", "contents": "Six years of experience with retrograde biopsy of intraureteral carcinoma using the Dormia stone basket. To confirm the cause of a small defect of the ureter and to determine preoperatively the histological grade of ureteral carcinoma a new technique of intraureteral biopsy was attempted with a Dormia stone basket. Of 8 biopsies performed on 7 patients with ureteral or pelvioureteral carcinoma only 1 failed to produce a fragment of tissue. Histological diagnosis was made in each of the 7 patients and histological grading in 5. Four patients with non-malignant defects of the lower ureter also were biopsied with this technique to ascertain the feasibility of this biopsy on minimal filling defects. In each patient a tiny piece of tissue was removed. Histological diagnosis could be made in 3 cases.", "PMID": 957494}
{"id": "pubmed23n0032_2755", "title": "Long-term results of cutaneous omento-ureterostomy.", "content": "Six of 11 patients have had 2 to 7 1/2 years of followup evaluation after cutaneous omento-ureterostomy. Results were excellent in 3 cases and good in 1, while ureteral stomal stenosis developed in the 2 patients irradiated for bladder cancer. Complete prevention of cutaneous ureterostomy stoma stenosis remains unachieved but the use of a plastic meatal dilator seems useful.", "contents": "Long-term results of cutaneous omento-ureterostomy. Six of 11 patients have had 2 to 7 1/2 years of followup evaluation after cutaneous omento-ureterostomy. Results were excellent in 3 cases and good in 1, while ureteral stomal stenosis developed in the 2 patients irradiated for bladder cancer. Complete prevention of cutaneous ureterostomy stoma stenosis remains unachieved but the use of a plastic meatal dilator seems useful.", "PMID": 957495}
{"id": "pubmed23n0032_2756", "title": "External sphincterotomy: its effect on erections.", "content": "External urethral sphincterotomy on 129 paraplegics had little effect on erections. In the 101 patients with post-injury erections only 2 had cessation of erectile capacity. Subjectively, 16 per cent had partial loss and 13 per cent had an increase. The remaining 69 per cent had no change. When the data were correlated with level and completeness of injury, age, time since injury, time since sphincterotomy, postoperative delayed hemorrhage and effect on leg and bladder spasms, no conclusions could be drawn concerning the etiology of altered erections.", "contents": "External sphincterotomy: its effect on erections. External urethral sphincterotomy on 129 paraplegics had little effect on erections. In the 101 patients with post-injury erections only 2 had cessation of erectile capacity. Subjectively, 16 per cent had partial loss and 13 per cent had an increase. The remaining 69 per cent had no change. When the data were correlated with level and completeness of injury, age, time since injury, time since sphincterotomy, postoperative delayed hemorrhage and effect on leg and bladder spasms, no conclusions could be drawn concerning the etiology of altered erections.", "PMID": 957497}
{"id": "pubmed23n0032_2757", "title": "Correlation between follicle stimulating hormone, luteinizing hormone, testosterone and 5-hydroxyindole acetic acid with sperm cell concentration.", "content": "Plasma follicle stimulating hormone, luteinizing hormone, testosterone, urinary 5-hydroxyindole acetic acid and 17-ketosteroids were measured in patients seen at an infertility clinic. Plasma follicle stimulating hormone and luteinizing hormone levels, and urinary 5-hydroxyindole acetic acid levels were increased in patients with sperm concentrations less than 10 times 10(6) per ml. The results suggest that in patients with sperm counts less than 10 times 10(6) per ml. there is not only impaired spermatogenesis but also decreased Leydig cell function. Urinary 17-ketosteroid levels were not related to sperm cell concentration.", "contents": "Correlation between follicle stimulating hormone, luteinizing hormone, testosterone and 5-hydroxyindole acetic acid with sperm cell concentration. Plasma follicle stimulating hormone, luteinizing hormone, testosterone, urinary 5-hydroxyindole acetic acid and 17-ketosteroids were measured in patients seen at an infertility clinic. Plasma follicle stimulating hormone and luteinizing hormone levels, and urinary 5-hydroxyindole acetic acid levels were increased in patients with sperm concentrations less than 10 times 10(6) per ml. The results suggest that in patients with sperm counts less than 10 times 10(6) per ml. there is not only impaired spermatogenesis but also decreased Leydig cell function. Urinary 17-ketosteroid levels were not related to sperm cell concentration.", "PMID": 957498}
{"id": "pubmed23n0032_2758", "title": "Extratesticular lipoma: report of 2 cases and a new classification.", "content": "We report 2 cases of intrascrotal extratesticular lipoma and propose a new classification. If the possible anatomic sites of origin and the histology of this neoplasm are considered during dissection the tumor can be excised without unnecessary orchiectomy when the blood supply of the testicle can be preserved.", "contents": "Extratesticular lipoma: report of 2 cases and a new classification. We report 2 cases of intrascrotal extratesticular lipoma and propose a new classification. If the possible anatomic sites of origin and the histology of this neoplasm are considered during dissection the tumor can be excised without unnecessary orchiectomy when the blood supply of the testicle can be preserved.", "PMID": 957499}
{"id": "pubmed23n0032_2759", "title": "Outpatient needle aspiration biopsy of the prostate.", "content": "The use of aspiration needle biopsy and needle biopsy of the prostate is described in 38 cases. Aspiration needle biopsy is a fast, safe and reliable procedure that requires no special equipment. It can be used as a preliminary office screening procedure in the diagnosis of carcinoma of the prostate.", "contents": "Outpatient needle aspiration biopsy of the prostate. The use of aspiration needle biopsy and needle biopsy of the prostate is described in 38 cases. Aspiration needle biopsy is a fast, safe and reliable procedure that requires no special equipment. It can be used as a preliminary office screening procedure in the diagnosis of carcinoma of the prostate.", "PMID": 957500}
{"id": "pubmed23n0032_2760", "title": "A study of 48 cases of incidental carcinoma of the prostate followed 10 years or longer.", "content": "We reviewed 48 cases of incidental carcinoma treated conservatively. Patients with poorly differentiated, multiple foci or with well differentiated, multiple foci had a poor prognosis, whereas those with well differentiated, few foci (1 to 3) had a good prognosis. We believe that patients with multiple foci (poorly or well differentiated) should be treated aggressively, either by radical prostatectomy or radiotherapy. However, we recommend conservative treatment for patients with well differentiated, few foci.", "contents": "A study of 48 cases of incidental carcinoma of the prostate followed 10 years or longer. We reviewed 48 cases of incidental carcinoma treated conservatively. Patients with poorly differentiated, multiple foci or with well differentiated, multiple foci had a poor prognosis, whereas those with well differentiated, few foci (1 to 3) had a good prognosis. We believe that patients with multiple foci (poorly or well differentiated) should be treated aggressively, either by radical prostatectomy or radiotherapy. However, we recommend conservative treatment for patients with well differentiated, few foci.", "PMID": 957501}
{"id": "pubmed23n0032_2761", "title": "Factors influencing sexual activity after prostatectomy: a prospective study.", "content": "Between 16 and 30 per cent of all prostatectomy patients become impotent after an operation for benign prostatic hyperplasia. Since the surgical technique does not seem to be the factor responsible for such a serious problem, more accentuated by the fact that this operation is becoming increasingly frequent with the increase in life expectancy, an assessment of 15 patients before and after prostatectomy is presented. With a statistical analysis of a structured interview (including a mini-Minnesota Multiphasic Personality Inventory test before and after the operation) 3 main differentiating factors emerged between the potent and the impotent group: 1) the level of anxiety exhibited by the patient, 2) whether the patients received an explanation about the surgery and its outcome prior to the operation and 3) the patient's general satisfaction with life.", "contents": "Factors influencing sexual activity after prostatectomy: a prospective study. Between 16 and 30 per cent of all prostatectomy patients become impotent after an operation for benign prostatic hyperplasia. Since the surgical technique does not seem to be the factor responsible for such a serious problem, more accentuated by the fact that this operation is becoming increasingly frequent with the increase in life expectancy, an assessment of 15 patients before and after prostatectomy is presented. With a statistical analysis of a structured interview (including a mini-Minnesota Multiphasic Personality Inventory test before and after the operation) 3 main differentiating factors emerged between the potent and the impotent group: 1) the level of anxiety exhibited by the patient, 2) whether the patients received an explanation about the surgery and its outcome prior to the operation and 3) the patient's general satisfaction with life.", "PMID": 957502}
{"id": "pubmed23n0032_2762", "title": "Contribution to therapeutic decisions of ratios, absolute values and other measures of calcium, magnesium, urate or oxalate balance in stone formers.", "content": "Investigation of multiple serum and urinary factors in 44 patients with calcium urinary stone disease confirmed a number of defects that have been described previously: elevation of mean serum calcium and uric acid above normal, and depression of mean serum magnesium. Urinary excretion of calcium and uric acid was increased and was increased and was probably related to food ingestion. Urinary magnesium also increased after eating but less than calcium, with the result that for most patients the magnesium to calcium x 100 ratio approached levels observed in stone formation. Urinary oxalate excretion was constant during the entire observation period and apparently was not affected by ingestion of a defined diet. Nine additional patients had persistent hypercalcemia owing to hyperparathyroidism (5 confirmed, 1 suspected), malignancy (2) and drug ingestion (1). Metabolic evaluation of patients with calcium urinary calculi continues to contribute to decisions regarding their best therapeutic regimen.", "contents": "Contribution to therapeutic decisions of ratios, absolute values and other measures of calcium, magnesium, urate or oxalate balance in stone formers. Investigation of multiple serum and urinary factors in 44 patients with calcium urinary stone disease confirmed a number of defects that have been described previously: elevation of mean serum calcium and uric acid above normal, and depression of mean serum magnesium. Urinary excretion of calcium and uric acid was increased and was increased and was probably related to food ingestion. Urinary magnesium also increased after eating but less than calcium, with the result that for most patients the magnesium to calcium x 100 ratio approached levels observed in stone formation. Urinary oxalate excretion was constant during the entire observation period and apparently was not affected by ingestion of a defined diet. Nine additional patients had persistent hypercalcemia owing to hyperparathyroidism (5 confirmed, 1 suspected), malignancy (2) and drug ingestion (1). Metabolic evaluation of patients with calcium urinary calculi continues to contribute to decisions regarding their best therapeutic regimen.", "PMID": 957503}
{"id": "pubmed23n0032_2763", "title": "Pentolinium in the management of autonomic hyperreflexia.", "content": "Patients with high spinal cord injuries frequently will develop a syndrome of autonomic hyperreflexia when stimulated by urological instrumentation. Severe sustained hypertension is the most hazardous component encountered during this reaction. Herein we report a series of patients exhibiting this condition who were managed safely and successfully by the use of the ganglionic blocking agent, pentolinium.", "contents": "Pentolinium in the management of autonomic hyperreflexia. Patients with high spinal cord injuries frequently will develop a syndrome of autonomic hyperreflexia when stimulated by urological instrumentation. Severe sustained hypertension is the most hazardous component encountered during this reaction. Herein we report a series of patients exhibiting this condition who were managed safely and successfully by the use of the ganglionic blocking agent, pentolinium.", "PMID": 957505}
{"id": "pubmed23n0032_2764", "title": "Hypoplasia of the major renal conduits.", "content": "An unusual case of a patient surviving a bilateral congenital anomaly of the major renal conduits is presented. Anatomical features of this case, considered with present embryological ideas in mind, support the concept of a continuum of disorders of the major renal conduits. The literature is reviewed and interesting microsurgical techniques with hypothermia enabling reconstruction of the collecting systems and renal preservation are discussed.", "contents": "Hypoplasia of the major renal conduits. An unusual case of a patient surviving a bilateral congenital anomaly of the major renal conduits is presented. Anatomical features of this case, considered with present embryological ideas in mind, support the concept of a continuum of disorders of the major renal conduits. The literature is reviewed and interesting microsurgical techniques with hypothermia enabling reconstruction of the collecting systems and renal preservation are discussed.", "PMID": 957506}
{"id": "pubmed23n0032_2765", "title": "Simultaneous adrenal and multiple bilateral renal cysts.", "content": "Benign cysts of the adrenal gland are rare. This is the first reported case of the simultaneous occurrence of an asymptomatic right adrenal cyst and bilateral multiple renal cysts. Excretory urograms with tomograms, renal aortography and ultrasound scanning were used to establish a non-invasive definitive diagnosis. There was no need for an operation since the lesions were benign.", "contents": "Simultaneous adrenal and multiple bilateral renal cysts. Benign cysts of the adrenal gland are rare. This is the first reported case of the simultaneous occurrence of an asymptomatic right adrenal cyst and bilateral multiple renal cysts. Excretory urograms with tomograms, renal aortography and ultrasound scanning were used to establish a non-invasive definitive diagnosis. There was no need for an operation since the lesions were benign.", "PMID": 957509}
{"id": "pubmed23n0032_2766", "title": "A renal cause for massive retroperitoneal hemorrhage--renal angiomyolipoma.", "content": "In any case of intra-abdominal trauma or hemorrhage a preoperative IVP is essential to determine the renal status. Preoperative catheter occlusion of the renal artery in these vascular tumors may be attempted to decrease blood loss. Stigmas of tuberous sclerosis should be sought in patients with vascular renal tumors, especially if they are bilateral. Conservation of renal tissue in tuberous sclerotic patients harboring renal angiomyolipomas is important.", "contents": "A renal cause for massive retroperitoneal hemorrhage--renal angiomyolipoma. In any case of intra-abdominal trauma or hemorrhage a preoperative IVP is essential to determine the renal status. Preoperative catheter occlusion of the renal artery in these vascular tumors may be attempted to decrease blood loss. Stigmas of tuberous sclerosis should be sought in patients with vascular renal tumors, especially if they are bilateral. Conservation of renal tissue in tuberous sclerotic patients harboring renal angiomyolipomas is important.", "PMID": 957510}
{"id": "pubmed23n0032_2767", "title": "[A case of psittacosis treated with rifampicin (author's transl)].", "content": "A 45-year-old male who had looked over pet-birds at a bird shop 11 days before, developed a high fever with chills. Without any response to cephalexin, he was admitted to the hospital, with the chief complaints of high fever and sever headache on the 8th day of illness. The chest X-ray films taken then revealed a fun-shaped ground glass-like shadow extending over S10 of the right lung. After the oral administration of 450 mg rifampicin on the 9th day of illness, he became a febrile within one day and was cured with the same daily doses for the following 10 days. Chlamydia was isolated from the peritoneal exudate of the mice inoculated with throat mucus of the patient taken prior to the administration of rifampicin. Complement fixation reaction for psittacosis was positive in a titer of 1 : 16 on the 11th day of illness and rose to 1 : 64 in a week.", "contents": "[A case of psittacosis treated with rifampicin (author's transl)]. A 45-year-old male who had looked over pet-birds at a bird shop 11 days before, developed a high fever with chills. Without any response to cephalexin, he was admitted to the hospital, with the chief complaints of high fever and sever headache on the 8th day of illness. The chest X-ray films taken then revealed a fun-shaped ground glass-like shadow extending over S10 of the right lung. After the oral administration of 450 mg rifampicin on the 9th day of illness, he became a febrile within one day and was cured with the same daily doses for the following 10 days. Chlamydia was isolated from the peritoneal exudate of the mice inoculated with throat mucus of the patient taken prior to the administration of rifampicin. Complement fixation reaction for psittacosis was positive in a titer of 1 : 16 on the 11th day of illness and rose to 1 : 64 in a week.", "PMID": 957515}
{"id": "pubmed23n0032_2768", "title": "[Studies on absorption and excretion of cyclacillin granules (vastcillin granules 'Takeda') (author's transl)].", "content": "Vastcillin granules 'Takeda', a cyclacillin (AC-PC) preparation for children were orally administered to nine children at a single dose of 10 mg/kg and its blood concentrations and urinary excretion were studied. From the results obtained, the following conclusions were obtained as to clinical dose and indications. (1) Absorption of AC-PC of Vastcillin granules is excellent . At 15 minutes after administration, 9.3 mcg/ml of AC-PC blood level was attained and its peak appeared 30 minutes after administration. The mean peak level was 17.6 mcg/ml.", "contents": "[Studies on absorption and excretion of cyclacillin granules (vastcillin granules 'Takeda') (author's transl)]. Vastcillin granules 'Takeda', a cyclacillin (AC-PC) preparation for children were orally administered to nine children at a single dose of 10 mg/kg and its blood concentrations and urinary excretion were studied. From the results obtained, the following conclusions were obtained as to clinical dose and indications. (1) Absorption of AC-PC of Vastcillin granules is excellent . At 15 minutes after administration, 9.3 mcg/ml of AC-PC blood level was attained and its peak appeared 30 minutes after administration. The mean peak level was 17.6 mcg/ml.", "PMID": 957520}
{"id": "pubmed23n0032_2769", "title": "[Studies on the disc method for the determination of bacterial sensitivity to ribostamycin (author's transl)].", "content": "The MICs of ribostamycin (RSM) were determined by the two-fold serial agar-dilution method of 109 bacterial strains of 20 species. The diameters of inhibition zones of these bacterial strains by the 50 mug RSM disc were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 approximately 6 hours and 3 approximately 4 hours culture methods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of RSM. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.", "contents": "[Studies on the disc method for the determination of bacterial sensitivity to ribostamycin (author's transl)]. The MICs of ribostamycin (RSM) were determined by the two-fold serial agar-dilution method of 109 bacterial strains of 20 species. The diameters of inhibition zones of these bacterial strains by the 50 mug RSM disc were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 approximately 6 hours and 3 approximately 4 hours culture methods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of RSM. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.", "PMID": 957521}
{"id": "pubmed23n0032_2770", "title": "Detection of DNA polymerase activity in Chlamydia psittaci.", "content": "DNA polymerase activities of intact and disrupted suspensions of the mature infectious, extracellular elementary bodies of the meningopneumonitis strain of Chlamydia psittaci were studied. Intact elementary bodies failed to incorporate labeled thymidine triphosphate (TTP), but homogenates of the organisms did incorporate TTP into the acid insoluble fraction; the reaction continued at a linear rate for 60 min and the newly synthesized DNA hybridized exclusively with DNA derived from C. psittaci elementary bodies. Synthesized DNA sedimented in 2 fractions in alkaline sucrose gradients, one in the 50S region and the other in the 10S region, corresponding to molecular weights of 2 X 10(7) and 5 X 10(5) daltons, respectively.", "contents": "Detection of DNA polymerase activity in Chlamydia psittaci. DNA polymerase activities of intact and disrupted suspensions of the mature infectious, extracellular elementary bodies of the meningopneumonitis strain of Chlamydia psittaci were studied. Intact elementary bodies failed to incorporate labeled thymidine triphosphate (TTP), but homogenates of the organisms did incorporate TTP into the acid insoluble fraction; the reaction continued at a linear rate for 60 min and the newly synthesized DNA hybridized exclusively with DNA derived from C. psittaci elementary bodies. Synthesized DNA sedimented in 2 fractions in alkaline sucrose gradients, one in the 50S region and the other in the 10S region, corresponding to molecular weights of 2 X 10(7) and 5 X 10(5) daltons, respectively.", "PMID": 957524}
{"id": "pubmed23n0032_2771", "title": "The endogenous secretin in chicken: minor physiological role in exocrine pancreatic secretion.", "content": "The action of secretin (SN) on the exocrine pancreas in the chicken was studied in comparison with the rat and dog. Hydrochloric acid (0.1 N), pepton (5%) and olive oil were introduced into the small intestine of conscious chickens with chronic pancreatic fistula and caused the volume and protein output to increase slightly in about two-thirds of the chickens tested. The percent increase of the volume output in anesthetized chickens by HCl instillation was only one-tenth of that in the rat. Acid extracts of the intestinal mucosa (80 mg) from chickens when given intravenously to rats caused the volume of secretion from the rat pancreas to increase about twofold, whereas the extracts from the dogs' and rats' intestine increased the volume output 5.9 and 5.5 times respectively. The increase of pancreatic secretion in chickens by intravenous injection of commercial SN was about one-third of that in the rat. The present studies indicate that SN or a SN-like hormone may be present in the chicken, but it plays only a minor physiological role.", "contents": "The endogenous secretin in chicken: minor physiological role in exocrine pancreatic secretion. The action of secretin (SN) on the exocrine pancreas in the chicken was studied in comparison with the rat and dog. Hydrochloric acid (0.1 N), pepton (5%) and olive oil were introduced into the small intestine of conscious chickens with chronic pancreatic fistula and caused the volume and protein output to increase slightly in about two-thirds of the chickens tested. The percent increase of the volume output in anesthetized chickens by HCl instillation was only one-tenth of that in the rat. Acid extracts of the intestinal mucosa (80 mg) from chickens when given intravenously to rats caused the volume of secretion from the rat pancreas to increase about twofold, whereas the extracts from the dogs' and rats' intestine increased the volume output 5.9 and 5.5 times respectively. The increase of pancreatic secretion in chickens by intravenous injection of commercial SN was about one-third of that in the rat. The present studies indicate that SN or a SN-like hormone may be present in the chicken, but it plays only a minor physiological role.", "PMID": 957528}
{"id": "pubmed23n0032_2772", "title": "Effect of dantrolene sodium on excitation-contraction coupling of frog toe muscle.", "content": "Dantrolene sodium (1.0 to 10.0 mug/ml) inhibited twitch tension, tetanus tension, and contracture induced by potassium and low concentration of caffeine in the frog toe muscles. The drug also inhibited rapid cooling contracture (RCC), i.e., contracture caused by rapidly cooling the muscle from room temperature to 0 degrees C after immersing it in Ringer's solution containing a subthreshold concentration of caffein. The drug also suppressed RCC in depolarized muscle fibres which were treated with high concentration of potassium. Comparing the effect of the drug on twitches augmented with nitrate and with caffeine, the former was more markedly inhibited than the latter. On the basis of these results, the possibility that the drug acts mainly by inhibiting the movement of \"trigger calcium\" which in turn releases calcium from the sarcoplasmic reticulum is discussed.", "contents": "Effect of dantrolene sodium on excitation-contraction coupling of frog toe muscle. Dantrolene sodium (1.0 to 10.0 mug/ml) inhibited twitch tension, tetanus tension, and contracture induced by potassium and low concentration of caffeine in the frog toe muscles. The drug also inhibited rapid cooling contracture (RCC), i.e., contracture caused by rapidly cooling the muscle from room temperature to 0 degrees C after immersing it in Ringer's solution containing a subthreshold concentration of caffein. The drug also suppressed RCC in depolarized muscle fibres which were treated with high concentration of potassium. Comparing the effect of the drug on twitches augmented with nitrate and with caffeine, the former was more markedly inhibited than the latter. On the basis of these results, the possibility that the drug acts mainly by inhibiting the movement of \"trigger calcium\" which in turn releases calcium from the sarcoplasmic reticulum is discussed.", "PMID": 957529}
{"id": "pubmed23n0032_2773", "title": "Interpretation of disaccharide-dependent electrical potential differences in the small intestine.", "content": "The nature of maltose- and trehalose-induced electrical potential increments across everted small intestines of toads were investigated. A Michaelis-Menten-like relation was seen between the amplitude of PD increments (deltaPD) and the mucosal concentration of disaccharides over a wide range of concentration, but, at a higher concentration range, Lineweaver-Burk type plot of data always deviated from the linear line for the low and moderate concentration range. The extrapolation of the linear segment of the plots intercepted the ordinate at the same point as that of the line for the glucose-induced potential increments. Both the disaccharide- and the glucose-evoked potentials were not additive and were equally sensitive to phlorizin. Tris depressed the disaccharide-evoked potentials to about the same extent as that of inhibition of enzyme activities. The amplitude and time course of the disaccharide-induced potentials, however, could not be accounted for by the mucosal concentration of liberated glucose. These qualitative and quantitative characteristics were explained properly on the basis of a simple well-type local pool for liberated glucose assumed to exist at the brush border. In conclusion, a close functional linkage between brush border membrane disaccharidase activities and the electrogenic hexose transport is well reflected in the disaccharide-evoked potentials in the small intestine.", "contents": "Interpretation of disaccharide-dependent electrical potential differences in the small intestine. The nature of maltose- and trehalose-induced electrical potential increments across everted small intestines of toads were investigated. A Michaelis-Menten-like relation was seen between the amplitude of PD increments (deltaPD) and the mucosal concentration of disaccharides over a wide range of concentration, but, at a higher concentration range, Lineweaver-Burk type plot of data always deviated from the linear line for the low and moderate concentration range. The extrapolation of the linear segment of the plots intercepted the ordinate at the same point as that of the line for the glucose-induced potential increments. Both the disaccharide- and the glucose-evoked potentials were not additive and were equally sensitive to phlorizin. Tris depressed the disaccharide-evoked potentials to about the same extent as that of inhibition of enzyme activities. The amplitude and time course of the disaccharide-induced potentials, however, could not be accounted for by the mucosal concentration of liberated glucose. These qualitative and quantitative characteristics were explained properly on the basis of a simple well-type local pool for liberated glucose assumed to exist at the brush border. In conclusion, a close functional linkage between brush border membrane disaccharidase activities and the electrogenic hexose transport is well reflected in the disaccharide-evoked potentials in the small intestine.", "PMID": 957530}
{"id": "pubmed23n0032_2774", "title": "A mechanochemical model for the steady and transient contractions of the skeletal muscle.", "content": "A mechanochemical model for muscle contraction was presented which consisted of three subsystems: the regulatory mechanism of contraction by Ca ion, the cross-bridge cycle coupled with actin-myosin interaction and the dynamics of contraction with an external load. It was assumed that both the rate constant of the cross-bridge cycle and the net force of the cross-bridge were linear functions of the sliding velocity. Most parameters in the model were determined from the experimental data, but several were estimated by simulation techniques. The model adequately described the force-load-velocity relation, the rates of energy and heat output as well as some basic mechanical properties of muscle. Not only the steady-state contraction but also the twitch response could be explained by the model. Time courses of tension and shortening during isometric and isotonic twitches were calculated by the model on a digital computer. The calculated curves agreed satisfactorily with the experimental ones obtained from the frog semitendinosus muscle. The rate of tension rise of the isometric twitch was shown to attain the peak at nearly the same time as does the calculated Ca concentration curve.", "contents": "A mechanochemical model for the steady and transient contractions of the skeletal muscle. A mechanochemical model for muscle contraction was presented which consisted of three subsystems: the regulatory mechanism of contraction by Ca ion, the cross-bridge cycle coupled with actin-myosin interaction and the dynamics of contraction with an external load. It was assumed that both the rate constant of the cross-bridge cycle and the net force of the cross-bridge were linear functions of the sliding velocity. Most parameters in the model were determined from the experimental data, but several were estimated by simulation techniques. The model adequately described the force-load-velocity relation, the rates of energy and heat output as well as some basic mechanical properties of muscle. Not only the steady-state contraction but also the twitch response could be explained by the model. Time courses of tension and shortening during isometric and isotonic twitches were calculated by the model on a digital computer. The calculated curves agreed satisfactorily with the experimental ones obtained from the frog semitendinosus muscle. The rate of tension rise of the isometric twitch was shown to attain the peak at nearly the same time as does the calculated Ca concentration curve.", "PMID": 957531}
{"id": "pubmed23n0032_2775", "title": "Effects of calcium ion on the rising phase of the action potential in rabbit sinoatrial node cells.", "content": "To clarify the role of Ca ion in the rising phase of the sinoatrial (S-A) node action potential, the sigmoidal relationship between the maximum rate of rise of the action potential and the maximum diastolic potential was examined at various concentrations of Ca. The membrane potential was changed by applying a current across a single sucrose gap. The sigmoidal curve shifted toward the positive potential without a change in maximum value when the Ca concentration was increased from nominal \"zero\" to 10 mM. Therefore, it is concluded that Ca ion modifies the inactivation process of Na current which is responsible for the rapid rising phase of the S-A node action potential. The duration of the action potential and the maximum diastolic potential were decreased with an increase in Ca concentration. The observation that the overshoot of the action potential increased by 12 mV for a tenfold increase in concentration of Ca (within the range of 0.1-5.0 mM) suggests that the inward current of Ca ion may be responsible for the overshoot of the S-A node action potential.", "contents": "Effects of calcium ion on the rising phase of the action potential in rabbit sinoatrial node cells. To clarify the role of Ca ion in the rising phase of the sinoatrial (S-A) node action potential, the sigmoidal relationship between the maximum rate of rise of the action potential and the maximum diastolic potential was examined at various concentrations of Ca. The membrane potential was changed by applying a current across a single sucrose gap. The sigmoidal curve shifted toward the positive potential without a change in maximum value when the Ca concentration was increased from nominal \"zero\" to 10 mM. Therefore, it is concluded that Ca ion modifies the inactivation process of Na current which is responsible for the rapid rising phase of the S-A node action potential. The duration of the action potential and the maximum diastolic potential were decreased with an increase in Ca concentration. The observation that the overshoot of the action potential increased by 12 mV for a tenfold increase in concentration of Ca (within the range of 0.1-5.0 mM) suggests that the inward current of Ca ion may be responsible for the overshoot of the S-A node action potential.", "PMID": 957532}
{"id": "pubmed23n0032_2776", "title": "Rauwolfia derivatives and breast cancer.", "content": "During the course of a collaborative study initiated in investigate the relationship of breast cancer and the use of hormones, it was possible to study the possible relationship of rauwolfia derivatives, mainly reserpine, to breast cancer. Interviews were administered to breast cancer patients admitted to five hospitals in the Baltimore metropolitan area. The study of reserpine use was conducted for a period of approximately one and a half years and information on reserpine use was obtained on 164 breast cancer cases. Each case was matched with a control with respect to age, color, hospital of admission, and admission date. An additional set of controls was drawn from women residing in the same neighborhood as the cases. Neither reserpine, methyldopa, nor thiazides were found to be significantly associated with breast cancer. The exclusion from the analysis of hospital controls with a diagnosis of hypertensive disease and their matched cases did not significantly alter the results. Those cases who used reserpine, used more for longer periods of time than did the controls, however this was judged inconclusive due to a considerable amount of missing data. After comparison of these results with previously reported studies, it did not appear that reserpine was strongly associated with breast cancer. However, because of methodological problems and differences between the studies, a possible small overall relationship could not be definitely excluded.", "contents": "Rauwolfia derivatives and breast cancer. During the course of a collaborative study initiated in investigate the relationship of breast cancer and the use of hormones, it was possible to study the possible relationship of rauwolfia derivatives, mainly reserpine, to breast cancer. Interviews were administered to breast cancer patients admitted to five hospitals in the Baltimore metropolitan area. The study of reserpine use was conducted for a period of approximately one and a half years and information on reserpine use was obtained on 164 breast cancer cases. Each case was matched with a control with respect to age, color, hospital of admission, and admission date. An additional set of controls was drawn from women residing in the same neighborhood as the cases. Neither reserpine, methyldopa, nor thiazides were found to be significantly associated with breast cancer. The exclusion from the analysis of hospital controls with a diagnosis of hypertensive disease and their matched cases did not significantly alter the results. Those cases who used reserpine, used more for longer periods of time than did the controls, however this was judged inconclusive due to a considerable amount of missing data. After comparison of these results with previously reported studies, it did not appear that reserpine was strongly associated with breast cancer. However, because of methodological problems and differences between the studies, a possible small overall relationship could not be definitely excluded.", "PMID": 957533}
{"id": "pubmed23n0032_2777", "title": "[Adenoma of the ciliary epithelium of unusual structure and localisation (authors transl)].", "content": "The authors describe an adenoma of the ciliary epithelium of low degree of malignacy. The tumor is clinically and histologically remarkable because of its uncommom site behind the iris and the abundance of mucoid stroma. The clinical data, the course as well as the histological findings are described and compared with the rare similar cases in the literature. Electron microscopic data of this kind of tumor are reported for the first time. The tumor are characterised by basal membranes, microvilli, desmosomes, occasional melanin granules, a well differentiated RER and specially by the massive content of intracytoplasmatic filaments.", "contents": "[Adenoma of the ciliary epithelium of unusual structure and localisation (authors transl)]. The authors describe an adenoma of the ciliary epithelium of low degree of malignacy. The tumor is clinically and histologically remarkable because of its uncommom site behind the iris and the abundance of mucoid stroma. The clinical data, the course as well as the histological findings are described and compared with the rare similar cases in the literature. Electron microscopic data of this kind of tumor are reported for the first time. The tumor are characterised by basal membranes, microvilli, desmosomes, occasional melanin granules, a well differentiated RER and specially by the massive content of intracytoplasmatic filaments.", "PMID": 957534}
{"id": "pubmed23n0032_2778", "title": "[Colloid bodies. A cause of vascular complications (author's transl)].", "content": "Optic disc colloid bodies are more common than previously supposed. They were noted to occur in 2.04% of unselected globes obtained at autopsy. Hemorrhages of the disc and juxtapapillary fundus are not infrequently found in association with disc colloid bodies. Superficial hemorrhages may be due to erosion of existing disc blood vessels by enlarging colloid bodies, on the other hand, juxtapapillary hemorrhages may occur from neovascularization induced by the colloid bodies. Ultrasound examination may be useful as an aid in diagnosing disc colloid bodies.", "contents": "[Colloid bodies. A cause of vascular complications (author's transl)]. Optic disc colloid bodies are more common than previously supposed. They were noted to occur in 2.04% of unselected globes obtained at autopsy. Hemorrhages of the disc and juxtapapillary fundus are not infrequently found in association with disc colloid bodies. Superficial hemorrhages may be due to erosion of existing disc blood vessels by enlarging colloid bodies, on the other hand, juxtapapillary hemorrhages may occur from neovascularization induced by the colloid bodies. Ultrasound examination may be useful as an aid in diagnosing disc colloid bodies.", "PMID": 957535}
{"id": "pubmed23n0032_2779", "title": "[The clinical misdiagnosis of chalazion (author's transl)].", "content": "Between 1966 and 1974, 1260 lidtumours were sent to the histological laboratory of the University Eye Hospital Hamburg. The clinical diagnosis \"chalazion\" in 138 cases could not be verified histologically in 33 cases. Other benign lesions were diagnosed 23 times and malignant lesions in 10 cases (4 times a Meibom's sebaceous cell carcinoma). The symptoms of all 7 Meibom's sebaceous cell carcinomatas diagnosed histologically during this time (the most common metastasizing lid-tumor in this series) were also analysed as they present the gravest diagnostic error for chalazia. Histologic examination of excised \"chalazion\"-material must be considered mandatory in all patients with a typical symptomatology, recurrence at the same site, high age, unilateral therapy-resistant keratoconjunctivities and involved regional lymphnodes.", "contents": "[The clinical misdiagnosis of chalazion (author's transl)]. Between 1966 and 1974, 1260 lidtumours were sent to the histological laboratory of the University Eye Hospital Hamburg. The clinical diagnosis \"chalazion\" in 138 cases could not be verified histologically in 33 cases. Other benign lesions were diagnosed 23 times and malignant lesions in 10 cases (4 times a Meibom's sebaceous cell carcinoma). The symptoms of all 7 Meibom's sebaceous cell carcinomatas diagnosed histologically during this time (the most common metastasizing lid-tumor in this series) were also analysed as they present the gravest diagnostic error for chalazia. Histologic examination of excised \"chalazion\"-material must be considered mandatory in all patients with a typical symptomatology, recurrence at the same site, high age, unilateral therapy-resistant keratoconjunctivities and involved regional lymphnodes.", "PMID": 957536}
{"id": "pubmed23n0032_2780", "title": "[Diabetic retinopathy, problems of documentation and evaluation (author's transl)].", "content": "Follow-up studies of diabetic retinopathy cause difficulties through the altering numbers and figures of the retinal changes. If the various pictures of retinopathy are changed furthermore by coagulation, we have to distinguish, between spontaneous improvements or deteriorations and changes which are due to therapy. Follow-up studies with various evaluation methods have shown that the chosen criteria do not lead to corresponding judgements. The visual acuity seems to be the only criteria with decisive and relatively objective statements.", "contents": "[Diabetic retinopathy, problems of documentation and evaluation (author's transl)]. Follow-up studies of diabetic retinopathy cause difficulties through the altering numbers and figures of the retinal changes. If the various pictures of retinopathy are changed furthermore by coagulation, we have to distinguish, between spontaneous improvements or deteriorations and changes which are due to therapy. Follow-up studies with various evaluation methods have shown that the chosen criteria do not lead to corresponding judgements. The visual acuity seems to be the only criteria with decisive and relatively objective statements.", "PMID": 957537}
{"id": "pubmed23n0032_2781", "title": "[The retinopathy of the childhood diabetic (author's transl)].", "content": "From investigations of 141 patients, diabetic since childhood, diabetic retinopathy (angiopathy), shows strikingly contrasting forms of progress: (a) thoroughly benign (retinopathia simplex; partly regressive); (b) malign (retinopathia proliferans and glomerulosclerosis). The tendency to progression of diabetic retino- and angiopathy appears to be hereditarily determined and is modified, later, only to a certain extent by environmental factors.", "contents": "[The retinopathy of the childhood diabetic (author's transl)]. From investigations of 141 patients, diabetic since childhood, diabetic retinopathy (angiopathy), shows strikingly contrasting forms of progress: (a) thoroughly benign (retinopathia simplex; partly regressive); (b) malign (retinopathia proliferans and glomerulosclerosis). The tendency to progression of diabetic retino- and angiopathy appears to be hereditarily determined and is modified, later, only to a certain extent by environmental factors.", "PMID": 957538}
{"id": "pubmed23n0032_2782", "title": "[Bilateral marked fundus changes with neovascularisation and subsequent detachment in association with thrombocythaemia (author's transl)].", "content": "Following radical hysterectomy a 65-year old patient developed massive preretinal vascular proliferation, similar in appearance to advanced proliferative diabetic retinopathy, which led to a traction detachment. Diabetes mellitus could not be established, and a thrombocyte count up to one million is discussed as a possible cause.", "contents": "[Bilateral marked fundus changes with neovascularisation and subsequent detachment in association with thrombocythaemia (author's transl)]. Following radical hysterectomy a 65-year old patient developed massive preretinal vascular proliferation, similar in appearance to advanced proliferative diabetic retinopathy, which led to a traction detachment. Diabetes mellitus could not be established, and a thrombocyte count up to one million is discussed as a possible cause.", "PMID": 957539}
{"id": "pubmed23n0032_2783", "title": "[Secondary disciform macular degeneration (author's transl)].", "content": "The disciform macular degeneration is in rare cases preceded by well-known diseases which have in common the destruction of the Bruch's membrane, recurrent haemorrhages with exudation from the choriocapillaris and later on ingrown of fibrovascular tissue into the subretinal space. The authors report on cases with chorioidal ruptures, vascularlike stripes and focal chorioiditis.", "contents": "[Secondary disciform macular degeneration (author's transl)]. The disciform macular degeneration is in rare cases preceded by well-known diseases which have in common the destruction of the Bruch's membrane, recurrent haemorrhages with exudation from the choriocapillaris and later on ingrown of fibrovascular tissue into the subretinal space. The authors report on cases with chorioidal ruptures, vascularlike stripes and focal chorioiditis.", "PMID": 957540}
{"id": "pubmed23n0032_2784", "title": "[Feeling of sand in the eyes and hormonal contraceptives (authors transl)].", "content": "By questionnaire forms the relation between the admission of taking antibaby pills and the subjective feeling of having sand in the eyes was checked. Out of 2201 patients from our group, aged between 9 and 84 years, 372 (16.9%) took hormonal contraceptives, 1812 (82.3%) denied the taking of contraceptives and 17 (0.8%) could not say. From the 372 patients who took hormonal contraceptives 37 (10%) complained of having the feeling of sand in the eyes, the other 335 (90%) denied having a feeling of sand in the eyes. The frequency of a subjective feeling of sand in the eyes was in the individual age groups independent of the subjective statement, of \"take hormonal contraceptives\" (chi2-test; P greater than 10%).", "contents": "[Feeling of sand in the eyes and hormonal contraceptives (authors transl)]. By questionnaire forms the relation between the admission of taking antibaby pills and the subjective feeling of having sand in the eyes was checked. Out of 2201 patients from our group, aged between 9 and 84 years, 372 (16.9%) took hormonal contraceptives, 1812 (82.3%) denied the taking of contraceptives and 17 (0.8%) could not say. From the 372 patients who took hormonal contraceptives 37 (10%) complained of having the feeling of sand in the eyes, the other 335 (90%) denied having a feeling of sand in the eyes. The frequency of a subjective feeling of sand in the eyes was in the individual age groups independent of the subjective statement, of \"take hormonal contraceptives\" (chi2-test; P greater than 10%).", "PMID": 957541}
{"id": "pubmed23n0032_2785", "title": "[Contusion and luxation of the eyeball with rupture of the optic fascicle in fronto-basal trauma (author's transl)].", "content": "A report is given of the pathological changes in two eyes which were enucleated because of anterior luxation with rupture of the orbital optic nerve two hours after a traffic accident with frontal head injury. In the first case there was rupture of the trabecular meshwork and Schlemm's canal, the ciliary body and epithelium of the pars plana, a giant retinal tear at the ora serrata and a retinal avulsion at the optic disc, a partial rupture of the peripheral and a complete one of the central lamina cribrosa with a longitudinal tear in the optic nerve which lead to a complete rupture of the orbital optic nerve together with its sheats 22 mm behind the globe. The lesions produced arised probably from a slightly oblique course of the frontal trauma with a suggested additional rotating force followed by a sudden anterior movement of the eye with subsequent luxation. In the second case there was a giant tear at the ora serrata and at the pars plana with dislocation of unpigmented epithelium of the pars plana over the ciliary body, a rupture of the optic fascicle behind the lamina cribrosa, and a rupture of the optic dura mater 16.5 mm behind the globe.", "contents": "[Contusion and luxation of the eyeball with rupture of the optic fascicle in fronto-basal trauma (author's transl)]. A report is given of the pathological changes in two eyes which were enucleated because of anterior luxation with rupture of the orbital optic nerve two hours after a traffic accident with frontal head injury. In the first case there was rupture of the trabecular meshwork and Schlemm's canal, the ciliary body and epithelium of the pars plana, a giant retinal tear at the ora serrata and a retinal avulsion at the optic disc, a partial rupture of the peripheral and a complete one of the central lamina cribrosa with a longitudinal tear in the optic nerve which lead to a complete rupture of the orbital optic nerve together with its sheats 22 mm behind the globe. The lesions produced arised probably from a slightly oblique course of the frontal trauma with a suggested additional rotating force followed by a sudden anterior movement of the eye with subsequent luxation. In the second case there was a giant tear at the ora serrata and at the pars plana with dislocation of unpigmented epithelium of the pars plana over the ciliary body, a rupture of the optic fascicle behind the lamina cribrosa, and a rupture of the optic dura mater 16.5 mm behind the globe.", "PMID": 957542}
{"id": "pubmed23n0032_2786", "title": "[The use of A-scan echography in the clinical diagnoses of persistent hyperplastic primary vitreous (PHPV) (AUTHOR'S TRANSL)].", "content": "In 15 eyes with PHPV (3 suspected cases) patterns of rudimentary hyaloid vessels were found by A-scan echography in 9 cases all under 7 years of age; although these vessels were seen ophthalmoscopically in only 1 case. This ultrasonographic finding appears to add an important contribution to the clinical diagnosis of PHPV, when the monolateral cataract is so dense as to observe other signs (elongated ciliary processes, retrolental fibrovascular tissue). It could also be demonstrated that the axial diameter of the lens remains smaller than that of the partner eye even when the cataract is intumescent with fattening of the anterior chamber. Contrary to our expectations, the axial length of the bulbus was increased in one third of the cases; while a relative microcornea was absent only in 2 eyes!", "contents": "[The use of A-scan echography in the clinical diagnoses of persistent hyperplastic primary vitreous (PHPV) (AUTHOR'S TRANSL)]. In 15 eyes with PHPV (3 suspected cases) patterns of rudimentary hyaloid vessels were found by A-scan echography in 9 cases all under 7 years of age; although these vessels were seen ophthalmoscopically in only 1 case. This ultrasonographic finding appears to add an important contribution to the clinical diagnosis of PHPV, when the monolateral cataract is so dense as to observe other signs (elongated ciliary processes, retrolental fibrovascular tissue). It could also be demonstrated that the axial diameter of the lens remains smaller than that of the partner eye even when the cataract is intumescent with fattening of the anterior chamber. Contrary to our expectations, the axial length of the bulbus was increased in one third of the cases; while a relative microcornea was absent only in 2 eyes!", "PMID": 957543}
{"id": "pubmed23n0032_2787", "title": "[Changes in the ocular muscles in progressive scleroderma (author's transl)].", "content": "One patient out of 13 with generalized scleroderma displayed related changes of ocular motility. Histologic examination of the external ocular muscles of one of our patients who had died, revealed a massive increase of the interstitial collagen, and various stages of muscular degeneration. These changes correspond closely with the histopathological findings in the myocardium.", "contents": "[Changes in the ocular muscles in progressive scleroderma (author's transl)]. One patient out of 13 with generalized scleroderma displayed related changes of ocular motility. Histologic examination of the external ocular muscles of one of our patients who had died, revealed a massive increase of the interstitial collagen, and various stages of muscular degeneration. These changes correspond closely with the histopathological findings in the myocardium.", "PMID": 957544}
{"id": "pubmed23n0032_2788", "title": "[Experiments on the toxicity of intra-ocular administered Gentamycin (author's transl)].", "content": "The compatibility with Gentamycin solution used for irrigation of the anterior chamber of the eye was studied in experiments performed on rabbits. The degree of damage was measured by counting the number of the undestroyed cells of the corneal endothelium after perfusion. We compared the number of undestroyed cells after perfusion with 0.25% Gentamycin solution, 0.50% Gentamycin solution and Ringer solution. The result of these experiments showed a clear reduction of the endothelial cells using a 0.50% solution of Gentamycin. On the other hand there was not found any considerable difference between the number of cells using a 0.25% solution of Gentamycin or using Ringer solution. Using both solutions there was only a very slight degree of damage to be observed.", "contents": "[Experiments on the toxicity of intra-ocular administered Gentamycin (author's transl)]. The compatibility with Gentamycin solution used for irrigation of the anterior chamber of the eye was studied in experiments performed on rabbits. The degree of damage was measured by counting the number of the undestroyed cells of the corneal endothelium after perfusion. We compared the number of undestroyed cells after perfusion with 0.25% Gentamycin solution, 0.50% Gentamycin solution and Ringer solution. The result of these experiments showed a clear reduction of the endothelial cells using a 0.50% solution of Gentamycin. On the other hand there was not found any considerable difference between the number of cells using a 0.25% solution of Gentamycin or using Ringer solution. Using both solutions there was only a very slight degree of damage to be observed.", "PMID": 957545}
{"id": "pubmed23n0032_2789", "title": "[Senile retinoschisis and cryocoagulation (author's transl)].", "content": "This is a report of experiences with the cryotherapy of senile retinoschisis. In spite of the prophylaxis retinal detachment occurred in 2 out of 30 eyes. The critical question, whether the prophylactic treatment is worthwhile or not, can be positively answered, when compared to the results of non treated retinoschisis (Shea et al., Okun and Cibis). A macular puckering was not seen.", "contents": "[Senile retinoschisis and cryocoagulation (author's transl)]. This is a report of experiences with the cryotherapy of senile retinoschisis. In spite of the prophylaxis retinal detachment occurred in 2 out of 30 eyes. The critical question, whether the prophylactic treatment is worthwhile or not, can be positively answered, when compared to the results of non treated retinoschisis (Shea et al., Okun and Cibis). A macular puckering was not seen.", "PMID": 957546}
{"id": "pubmed23n0032_2790", "title": "[Iridoschisis (author's transl)].", "content": "The alterations characteristic for Iridoschisis (type I and II) are discussed in the light of our own observations. Iridoschisis must be differentiated from the essential progressive iris atrophy, from the dysgenesis mesodermalis corneae et iridis (Rieger), the Chandler syndrome and from the genuine polycoria. Because of the genetic inter-relationships between Iridoschisis, essential promalis corneae and iridis, a predisposition for the occurrence of these alterations has to be taken into account.", "contents": "[Iridoschisis (author's transl)]. The alterations characteristic for Iridoschisis (type I and II) are discussed in the light of our own observations. Iridoschisis must be differentiated from the essential progressive iris atrophy, from the dysgenesis mesodermalis corneae et iridis (Rieger), the Chandler syndrome and from the genuine polycoria. Because of the genetic inter-relationships between Iridoschisis, essential promalis corneae and iridis, a predisposition for the occurrence of these alterations has to be taken into account.", "PMID": 957547}
{"id": "pubmed23n0032_2791", "title": "[Clinical contribution to Jaffe's syndrome (author's transl)].", "content": "The vitreo-retinal traction syndrome, described by Jaffe in 1967 represents a rare complication of idiopathic posterior vitreal detachment. In this vitreo-retinal disease process, which is ophthalmoscopically so characteristic and in the early phase so surprisingly symptom-free, and which only occurs in this form and to this extent following spontaneous intravitreal fibroplasia, we can discern 3 distinct stages, although transitional phases also occur. Vitreal and retinal changes occur which are developed to a varying extent in the 3 stages. Reported are 2 cases which were followed clinically through all 3 stages.", "contents": "[Clinical contribution to Jaffe's syndrome (author's transl)]. The vitreo-retinal traction syndrome, described by Jaffe in 1967 represents a rare complication of idiopathic posterior vitreal detachment. In this vitreo-retinal disease process, which is ophthalmoscopically so characteristic and in the early phase so surprisingly symptom-free, and which only occurs in this form and to this extent following spontaneous intravitreal fibroplasia, we can discern 3 distinct stages, although transitional phases also occur. Vitreal and retinal changes occur which are developed to a varying extent in the 3 stages. Reported are 2 cases which were followed clinically through all 3 stages.", "PMID": 957548}
{"id": "pubmed23n0032_2792", "title": "[A simple method to check alternating fixation in penalisation (author's transl)].", "content": "In addition to his glasses worn for penalisation, the patient receives spectacles with a red and green glass. Asked to fix the lamp of the examinator, the patient sees the light either red or green, according to which eye is fixing on the light.", "contents": "[A simple method to check alternating fixation in penalisation (author's transl)]. In addition to his glasses worn for penalisation, the patient receives spectacles with a red and green glass. Asked to fix the lamp of the examinator, the patient sees the light either red or green, according to which eye is fixing on the light.", "PMID": 957549}
{"id": "pubmed23n0032_2793", "title": "[The bilateral paralysis of the abducens nerve. A contribution to industrial medical judgement (author's transl)].", "content": "After a brief statistical survey the anotomic basis is explained in a broad outline. In the description of a case with bilateral paralysis of the abducens nerve, the suffering of the patient in his working life is examined and the phenomenon of inacurrate depth projection, of \"pointing past\", is discussed. As a result the suitability for employment is more highly reduced for those suffering from bilateral paralysis of the abducens nerve, as compared to persons with one eye. During the report a 16 mm Color film was shown, showing the patient before and after the O'Connor operation. The possibility of keeping a progress or control report, of patients suffering from inacurrate depth perception, was demonstrated.", "contents": "[The bilateral paralysis of the abducens nerve. A contribution to industrial medical judgement (author's transl)]. After a brief statistical survey the anotomic basis is explained in a broad outline. In the description of a case with bilateral paralysis of the abducens nerve, the suffering of the patient in his working life is examined and the phenomenon of inacurrate depth projection, of \"pointing past\", is discussed. As a result the suitability for employment is more highly reduced for those suffering from bilateral paralysis of the abducens nerve, as compared to persons with one eye. During the report a 16 mm Color film was shown, showing the patient before and after the O'Connor operation. The possibility of keeping a progress or control report, of patients suffering from inacurrate depth perception, was demonstrated.", "PMID": 957550}
{"id": "pubmed23n0032_2794", "title": "[Spastic entropion of the upperlid. A case observed in practice (author's transl)].", "content": "A 53-years-old patient was observed because of spastic entropion. This was incontestably a nonscarring rolling-inwards of the upper lid.", "contents": "[Spastic entropion of the upperlid. A case observed in practice (author's transl)]. A 53-years-old patient was observed because of spastic entropion. This was incontestably a nonscarring rolling-inwards of the upper lid.", "PMID": 957551}
{"id": "pubmed23n0032_2795", "title": "[Testing of macular function (author's transl)].", "content": "The readaptation time was used to test the function of the macula. After illumination of the macula with an ophthalmoscope the time was measured until the initial visual acuity was reached. Our findings are that the readaptation time of patients with degenerative lesions of the macula is significantly prolonged in comparison with normal controls. Furthermore the readaptation time in cases of exsudative-proliferative processes was longer than in cases of dry lesions of the macula. In clinical practice this method gives a good estimate of the functional state of the macula.", "contents": "[Testing of macular function (author's transl)]. The readaptation time was used to test the function of the macula. After illumination of the macula with an ophthalmoscope the time was measured until the initial visual acuity was reached. Our findings are that the readaptation time of patients with degenerative lesions of the macula is significantly prolonged in comparison with normal controls. Furthermore the readaptation time in cases of exsudative-proliferative processes was longer than in cases of dry lesions of the macula. In clinical practice this method gives a good estimate of the functional state of the macula.", "PMID": 957552}
{"id": "pubmed23n0032_2796", "title": "[Intraocular pressure in snorkling and diving (author's transl)].", "content": "The reactions of the intraocular pressure (i.o.p) in snorkling and scuba diving were studies on 29 healthy subjects. A patient with chronic glaucoma simplex was examined in a pressure tank. The results confirm the author's opinion, that a well compensated chronic glaucoma simplex with intact discs and fields does not exclude fitness to dive. However, a diver with narrow angel glaucoma risks sudden rise in i.o.p. and acute glaucoma while ascending to the surface. Persons, who are examined for fitness to dive, should be seen by an ophthalmologist, if they have a glaucoma or if they are hypermetropic and over 40 years of age.", "contents": "[Intraocular pressure in snorkling and diving (author's transl)]. The reactions of the intraocular pressure (i.o.p) in snorkling and scuba diving were studies on 29 healthy subjects. A patient with chronic glaucoma simplex was examined in a pressure tank. The results confirm the author's opinion, that a well compensated chronic glaucoma simplex with intact discs and fields does not exclude fitness to dive. However, a diver with narrow angel glaucoma risks sudden rise in i.o.p. and acute glaucoma while ascending to the surface. Persons, who are examined for fitness to dive, should be seen by an ophthalmologist, if they have a glaucoma or if they are hypermetropic and over 40 years of age.", "PMID": 957553}
{"id": "pubmed23n0032_2797", "title": "[Two cases of external ophthalmomyasis (author's transl)].", "content": "After a brief survey of Hungarian references the author reports on two cases of ophthalmomyasis externa. The organism was shown to be present oestrus ovis in one instance. The treatment with carbon-tetrachloride might be considered as a new and efficacious therapy.", "contents": "[Two cases of external ophthalmomyasis (author's transl)]. After a brief survey of Hungarian references the author reports on two cases of ophthalmomyasis externa. The organism was shown to be present oestrus ovis in one instance. The treatment with carbon-tetrachloride might be considered as a new and efficacious therapy.", "PMID": 957554}
{"id": "pubmed23n0032_2798", "title": "[A further modification of the Flieringa-ring (author's transl)].", "content": "A modified Flieringa-ring is demonstrated for the performance of a combined cataract-glaucoma surgery, by using a corneal incision and the Fronimopoulos modification of the Elliot trepanation.", "contents": "[A further modification of the Flieringa-ring (author's transl)]. A modified Flieringa-ring is demonstrated for the performance of a combined cataract-glaucoma surgery, by using a corneal incision and the Fronimopoulos modification of the Elliot trepanation.", "PMID": 957555}
{"id": "pubmed23n0032_2799", "title": "[Angled micro-scissors for after-cataract surgery (author's transl)].", "content": "A report is given on new micro-scissors for after-cataract operations. Its shape permits a perfect direction of incision in the pupillary region not only form a temporally placed corneal incision but also from all other meridians without its handling being impeded by the orbital border or th nose of the patient. Especially in certain injury sequels and other postoperative conditions the scissors extend the operative possibilities.", "contents": "[Angled micro-scissors for after-cataract surgery (author's transl)]. A report is given on new micro-scissors for after-cataract operations. Its shape permits a perfect direction of incision in the pupillary region not only form a temporally placed corneal incision but also from all other meridians without its handling being impeded by the orbital border or th nose of the patient. Especially in certain injury sequels and other postoperative conditions the scissors extend the operative possibilities.", "PMID": 957556}
{"id": "pubmed23n0032_2800", "title": "[Reading aids for blind persons. Technical possibilities, practice up to now, and future development (author's transl)].", "content": "This report deals with electronic reading aids enabling patients with a visual acuity of less than 1/50 to read black-and-white-printed matter. A survey on experience with the Optacon from 1971 on is furnished and the chances of vocational training and the use of supplementary aids. The very handy Optacon (weight: 2 kg) consists of a miniature opto-electronic camera, and a tactile stimulator area consisting of 144 tiny metal rods. The development of another very useful instrument in the hands of the blind looks toward completion: The Braille-Converter. Its functions in short: A TV camera with an intermediate computer which transfers optical information into braille or spoken language.", "contents": "[Reading aids for blind persons. Technical possibilities, practice up to now, and future development (author's transl)]. This report deals with electronic reading aids enabling patients with a visual acuity of less than 1/50 to read black-and-white-printed matter. A survey on experience with the Optacon from 1971 on is furnished and the chances of vocational training and the use of supplementary aids. The very handy Optacon (weight: 2 kg) consists of a miniature opto-electronic camera, and a tactile stimulator area consisting of 144 tiny metal rods. The development of another very useful instrument in the hands of the blind looks toward completion: The Braille-Converter. Its functions in short: A TV camera with an intermediate computer which transfers optical information into braille or spoken language.", "PMID": 957558}
{"id": "pubmed23n0032_2801", "title": "[Etiology and final clinical cause for 1000 enucleations. (A clinico-pathologic study) (author's transl)].", "content": "From 1969-1974 1000 unselected enucleated globes have been examined histopathologically. 277 derive from the University Eye Hospital in Hamburg, 723 from various Eye Hospitals in northern and southern Germany. They originate from 589 men and 408 women, three times the sex was unknown. 86 globes had to be removed from children less than 15 years old. 6 groups of etiologies have been distinguished: trauma (308), histologically confirmed neoplastic disease (281), ocular manifestations of systemic diseases (diabetes mellitus, occlusions of central retinal vessels presumably following generalized vascular disease etc.: 128), \"operative ocular disease\" (164), primary inflammatory disease (71), miscellaneous (malformations, high myopia, pseudo-glioma and pseudo-melanoma: 48). The etiology \"operative ocular disease\" consists of 67 primary glaucomas (57 adults, 10 buphthalmus), 41 idiopathic cataracts (7 of these congenital) and 3 primary corneal dystrophies, as well as 53 cases of primary retinal detachment. Among the 281 neoplastic diseases, there are 238 primary intraocular malignant melanomas of the uvea, 18 retinoblastomas, 4 primary reticulumcellsarcomas of the retina, 2 choroidal nevi, 10 intraocular metastases and 9 orbital tumors. 16 enucleations among the 1000 enucleations have been performed for pseudo-gliomas (5 x Coats disease, 5 x persistent primary hyperplastic vitreous, 2 x retrolental fibroplasia, others 4 x). The manifestations of systemic disease are consisting of 68 central retinal vein-occlusions, 30 complications of diabetes mellitus and 10 central retinal artery occlusions as well as 20 other generalized diseases. A primary inflammatory disease led to enucleation 50 times due to an intraocular process, 5 times due to scleritis and 18 times as a consequence of keratitis (including 13 times herpes simplex). As the final clinical cause for enucleation the following categories have been elaborated: secondary glaucomas (416), clinical diagnosis of \"tumor\" (275), atrophy and phthisis bulbi (118), inflammation (112), acute trauma to 4 weeks after the accident (72), others (7). In conclusion the central role of rubeosis iridis leading to secondary angle closure glaucoma is emphasized. This process presents a challenge to ophthalmologic research. Finally the significance of early surgery for primary angle closure glaucomas and for complete restoration of the anterior chamber after trauma and any intraocular procedure is stressed.", "contents": "[Etiology and final clinical cause for 1000 enucleations. (A clinico-pathologic study) (author's transl)]. From 1969-1974 1000 unselected enucleated globes have been examined histopathologically. 277 derive from the University Eye Hospital in Hamburg, 723 from various Eye Hospitals in northern and southern Germany. They originate from 589 men and 408 women, three times the sex was unknown. 86 globes had to be removed from children less than 15 years old. 6 groups of etiologies have been distinguished: trauma (308), histologically confirmed neoplastic disease (281), ocular manifestations of systemic diseases (diabetes mellitus, occlusions of central retinal vessels presumably following generalized vascular disease etc.: 128), \"operative ocular disease\" (164), primary inflammatory disease (71), miscellaneous (malformations, high myopia, pseudo-glioma and pseudo-melanoma: 48). The etiology \"operative ocular disease\" consists of 67 primary glaucomas (57 adults, 10 buphthalmus), 41 idiopathic cataracts (7 of these congenital) and 3 primary corneal dystrophies, as well as 53 cases of primary retinal detachment. Among the 281 neoplastic diseases, there are 238 primary intraocular malignant melanomas of the uvea, 18 retinoblastomas, 4 primary reticulumcellsarcomas of the retina, 2 choroidal nevi, 10 intraocular metastases and 9 orbital tumors. 16 enucleations among the 1000 enucleations have been performed for pseudo-gliomas (5 x Coats disease, 5 x persistent primary hyperplastic vitreous, 2 x retrolental fibroplasia, others 4 x). The manifestations of systemic disease are consisting of 68 central retinal vein-occlusions, 30 complications of diabetes mellitus and 10 central retinal artery occlusions as well as 20 other generalized diseases. A primary inflammatory disease led to enucleation 50 times due to an intraocular process, 5 times due to scleritis and 18 times as a consequence of keratitis (including 13 times herpes simplex). As the final clinical cause for enucleation the following categories have been elaborated: secondary glaucomas (416), clinical diagnosis of \"tumor\" (275), atrophy and phthisis bulbi (118), inflammation (112), acute trauma to 4 weeks after the accident (72), others (7). In conclusion the central role of rubeosis iridis leading to secondary angle closure glaucoma is emphasized. This process presents a challenge to ophthalmologic research. Finally the significance of early surgery for primary angle closure glaucomas and for complete restoration of the anterior chamber after trauma and any intraocular procedure is stressed.", "PMID": 957559}
{"id": "pubmed23n0032_2802", "title": "[Auto-allo-keratoplasty (with special reference to chondrokeratoprosthesis procedure) (author's transl)].", "content": "Biological, rather then purely technical approach seems to be very essential to ensure better results in alloplasty of the cornea. The diseased corneal tissue (especially, after corneal burns) usually fails to accept a keratoprosthesis permanently. The cornea must, therefore, be biologically reconstructed by transplantation of autogenous tissue. Autogenous fascia lata, periosteum, sclera and auricular cartilage have been tried in the present work to surve the purpose. Autogenous sclera and cartilage proved to be best materials, the latter having an important advantage of being easily obtainable. Experimental studies (including hystological control) proved the method of \"Chondrokeratoplasty\" to be well founded. During the past four years the method has been used on the eyes of 18 patients with most severe corneal lesions unmanageable by the usual graft technique. The Keratoprosthesis must be buried under the cartilage disk until the adequate nutrition is developed and full vitality of the graft is achieved. Only then the cartilage disk is trephined in its centre and the optical part of the keratoprosthesis is exposed. Technically, the procedure is much simpler as compared to the procedure of odonto-keratoprosthesis by strampelly. The results seem to be highly encouraging. So far there was no extrusion after chondrokeratoprosthesis procedure.", "contents": "[Auto-allo-keratoplasty (with special reference to chondrokeratoprosthesis procedure) (author's transl)]. Biological, rather then purely technical approach seems to be very essential to ensure better results in alloplasty of the cornea. The diseased corneal tissue (especially, after corneal burns) usually fails to accept a keratoprosthesis permanently. The cornea must, therefore, be biologically reconstructed by transplantation of autogenous tissue. Autogenous fascia lata, periosteum, sclera and auricular cartilage have been tried in the present work to surve the purpose. Autogenous sclera and cartilage proved to be best materials, the latter having an important advantage of being easily obtainable. Experimental studies (including hystological control) proved the method of \"Chondrokeratoplasty\" to be well founded. During the past four years the method has been used on the eyes of 18 patients with most severe corneal lesions unmanageable by the usual graft technique. The Keratoprosthesis must be buried under the cartilage disk until the adequate nutrition is developed and full vitality of the graft is achieved. Only then the cartilage disk is trephined in its centre and the optical part of the keratoprosthesis is exposed. Technically, the procedure is much simpler as compared to the procedure of odonto-keratoprosthesis by strampelly. The results seem to be highly encouraging. So far there was no extrusion after chondrokeratoprosthesis procedure.", "PMID": 957560}
{"id": "pubmed23n0032_2803", "title": "[Facts for the muscle-reflex-hypothesis of squint. (The importance of asymmetry in the structure of the eyes for the pathogenesis of concomitant squint) (author's transl)].", "content": "Concomitant squint is regarded as result of a congenital dissymmetry in the structure of the eyes or of the oculomotor apparatus and of an insufficiency of the optomotor reflexes. Squint develops, when 1. the anomalies of the structure of the eye-muscles or of their insertions are so considerable that binocular vision cannot be maintained by fusional and convergence-reflexes; 2. the optomotor reflexes are too weak in order to compensate functionally even insignificant structural deviations of the extraocular muscles or 3. the optomotor reflex-mechanisms are disturbed to such a degree that binocular cooperation becomes impossible although the anatomical conditions are normal. Children with the symptoms of \"potential squint\" (ametropia, heterophoria, pseudo-ectopia of the macula) need special ophthalmological care. Using prisms early and operating in time one can stop the monocular vision becoming established.", "contents": "[Facts for the muscle-reflex-hypothesis of squint. (The importance of asymmetry in the structure of the eyes for the pathogenesis of concomitant squint) (author's transl)]. Concomitant squint is regarded as result of a congenital dissymmetry in the structure of the eyes or of the oculomotor apparatus and of an insufficiency of the optomotor reflexes. Squint develops, when 1. the anomalies of the structure of the eye-muscles or of their insertions are so considerable that binocular vision cannot be maintained by fusional and convergence-reflexes; 2. the optomotor reflexes are too weak in order to compensate functionally even insignificant structural deviations of the extraocular muscles or 3. the optomotor reflex-mechanisms are disturbed to such a degree that binocular cooperation becomes impossible although the anatomical conditions are normal. Children with the symptoms of \"potential squint\" (ametropia, heterophoria, pseudo-ectopia of the macula) need special ophthalmological care. Using prisms early and operating in time one can stop the monocular vision becoming established.", "PMID": 957561}
{"id": "pubmed23n0032_2804", "title": "[Pathogenesis and treatment of diabetic retinopathy (author's transl)].", "content": "The initial disorder leading to any kind of diabetic retinopathy is hypoxia. After exhaustion of the compensatory autoregulation (vasodilatation and increase of the volume flow), the blood-retina barrier breaks down, and parts of the central retinal capillary network fall out (pre-retinopathy). In the non-proliferative and pre-proliferative stage, either disturbance of permeability or occlusion of capillaries is the prevailing process. The stage and predominance of one of the two pathogenic factors, which eventually may be jointed by vascular proliferation, are decisive for the application of light coagulation. The plan of the procedure must be based upon fluorescence angiography. If all progressive changes are treated, and fluorescence angiographic controls are made in intervals of several weeks or months, according to the stage and form (primary exsudative or primary occlusive) of the retinopathy, this procedure is preferable to the coagulation of big retinal areas (retinal ablation), and a maximum of retina with normal vascularisation can be saved. Medical treatment comprising calcium phosphate, the vasoprotecting Doxium, and clofibrate as a depressor of the lipid level in the blood, play a secondary role. Finally, hypophysectomy could not maintain its place in modern treatment of diabetic retinopathy.", "contents": "[Pathogenesis and treatment of diabetic retinopathy (author's transl)]. The initial disorder leading to any kind of diabetic retinopathy is hypoxia. After exhaustion of the compensatory autoregulation (vasodilatation and increase of the volume flow), the blood-retina barrier breaks down, and parts of the central retinal capillary network fall out (pre-retinopathy). In the non-proliferative and pre-proliferative stage, either disturbance of permeability or occlusion of capillaries is the prevailing process. The stage and predominance of one of the two pathogenic factors, which eventually may be jointed by vascular proliferation, are decisive for the application of light coagulation. The plan of the procedure must be based upon fluorescence angiography. If all progressive changes are treated, and fluorescence angiographic controls are made in intervals of several weeks or months, according to the stage and form (primary exsudative or primary occlusive) of the retinopathy, this procedure is preferable to the coagulation of big retinal areas (retinal ablation), and a maximum of retina with normal vascularisation can be saved. Medical treatment comprising calcium phosphate, the vasoprotecting Doxium, and clofibrate as a depressor of the lipid level in the blood, play a secondary role. Finally, hypophysectomy could not maintain its place in modern treatment of diabetic retinopathy.", "PMID": 957562}
{"id": "pubmed23n0032_2805", "title": "[Congenital grouped pigmentation of the retina (author's transl)].", "content": "In this disease entity we usually encounter black, sharply demarcated pigmented patches, occurring in groups and increasing in size toward the periphery. In the majority of cases the anomaly is unilateral (84%). The pigmentation may be found in one single sector (59%), in several separate sectors (27%) or distributed over the entire fundus (11%). Histology reveals pigment epithelial cells with hypertrophy and distinct irregularity in size and shape being arranged in one or two layers. Hyperpigmentation also exists with pigment granules of various size and distribution.", "contents": "[Congenital grouped pigmentation of the retina (author's transl)]. In this disease entity we usually encounter black, sharply demarcated pigmented patches, occurring in groups and increasing in size toward the periphery. In the majority of cases the anomaly is unilateral (84%). The pigmentation may be found in one single sector (59%), in several separate sectors (27%) or distributed over the entire fundus (11%). Histology reveals pigment epithelial cells with hypertrophy and distinct irregularity in size and shape being arranged in one or two layers. Hyperpigmentation also exists with pigment granules of various size and distribution.", "PMID": 957563}
{"id": "pubmed23n0032_2806", "title": "[A comport instrument for infrared examination of the eye (author's transl)].", "content": "A description is given of a comport infrared viewer recommended for clinical use in Ophthalmology. With this infrared viewer, an infrared slit lamp for advanced infrared diagnosis was developed.", "contents": "[A comport instrument for infrared examination of the eye (author's transl)]. A description is given of a comport infrared viewer recommended for clinical use in Ophthalmology. With this infrared viewer, an infrared slit lamp for advanced infrared diagnosis was developed.", "PMID": 957564}
{"id": "pubmed23n0032_2807", "title": "[Application of infrared radiometry on the eye (author's transl)].", "content": "The non-contact determination of temperature by infrared radiometry allows the focal measuring of the surface temperature of the radiation source. In 119 patients the surface temperature in the middle of the cornea was measured by this method. The average temperatures were 33.50 degrees C (+/-0.91) in men and 33.74 degrees C (+/-0.91) in women. Further clinical testing is suggested first in diseases of the oculo-orbital region and secondly by means of a connected recorder to study the dynamic temperature reaction under the influence of different parameters.", "contents": "[Application of infrared radiometry on the eye (author's transl)]. The non-contact determination of temperature by infrared radiometry allows the focal measuring of the surface temperature of the radiation source. In 119 patients the surface temperature in the middle of the cornea was measured by this method. The average temperatures were 33.50 degrees C (+/-0.91) in men and 33.74 degrees C (+/-0.91) in women. Further clinical testing is suggested first in diseases of the oculo-orbital region and secondly by means of a connected recorder to study the dynamic temperature reaction under the influence of different parameters.", "PMID": 957565}
{"id": "pubmed23n0032_2808", "title": "[Contribution to the problem of optic nerve excavation (author's transl)].", "content": "To study opinions on the formation of optic nerve head excavation we had the opportunity of examining microscopic sections of the papilla of a 10-month-old baby who showed bilateral considerable border excavation without the presence of hydrophthalmus or increased intraocular pressure. The thickened lamina cribrosa showed centrally no perforations so that the nerve fibres had to avoid the unperforated parts of the lamina. According to the size and position of such unperforated laminar areas, types of excavation with various defects on the optic nerve head resulted. By coincidence of both components in the optic nerve stem (increased connective tissue growth, and formation of a small vascular bundle), considerable collections of mesenchyme could occur which then resulted in a spreading apart of the optic nerve fibres. As long as neuroglia formation has not filled the gaps in the nervous tissue originally, a secondary growth of glial tissue can follow an original connective tissue-caused defect formation. Hence the deposits of glia in the excavated optic nerve head would be not the cause, but rather the effect of defect formation.", "contents": "[Contribution to the problem of optic nerve excavation (author's transl)]. To study opinions on the formation of optic nerve head excavation we had the opportunity of examining microscopic sections of the papilla of a 10-month-old baby who showed bilateral considerable border excavation without the presence of hydrophthalmus or increased intraocular pressure. The thickened lamina cribrosa showed centrally no perforations so that the nerve fibres had to avoid the unperforated parts of the lamina. According to the size and position of such unperforated laminar areas, types of excavation with various defects on the optic nerve head resulted. By coincidence of both components in the optic nerve stem (increased connective tissue growth, and formation of a small vascular bundle), considerable collections of mesenchyme could occur which then resulted in a spreading apart of the optic nerve fibres. As long as neuroglia formation has not filled the gaps in the nervous tissue originally, a secondary growth of glial tissue can follow an original connective tissue-caused defect formation. Hence the deposits of glia in the excavated optic nerve head would be not the cause, but rather the effect of defect formation.", "PMID": 957566}
{"id": "pubmed23n0032_2809", "title": "[A rat holder for experimental fundus and anterior segment photography (author's transl)].", "content": "We conceived and manufactured a holder for rats. It is a useful little apparatus for some experiments on rats as well as for its fundus photography, angiographies included. The utility of our device is discussed and illustrated.", "contents": "[A rat holder for experimental fundus and anterior segment photography (author's transl)]. We conceived and manufactured a holder for rats. It is a useful little apparatus for some experiments on rats as well as for its fundus photography, angiographies included. The utility of our device is discussed and illustrated.", "PMID": 957567}
{"id": "pubmed23n0032_2810", "title": "[Mycosis fungoides affecting the eye and internal organs (author's transl)].", "content": "In two patients with mycosis fungoides the following were found in the region of the eye: Infiltration of the eyelid (sometimes board-like), infiltrations and swellings of the connective tissue, orbital infiltrations (hard as a board) with secondary glaucoma, atrophy of the optic nerve, amaurosis, Descemet spots, cells in the aqueous humour, preretinal opacities of the vitreous body), intussusception of retinal vessels, grey infiltration of the retina.", "contents": "[Mycosis fungoides affecting the eye and internal organs (author's transl)]. In two patients with mycosis fungoides the following were found in the region of the eye: Infiltration of the eyelid (sometimes board-like), infiltrations and swellings of the connective tissue, orbital infiltrations (hard as a board) with secondary glaucoma, atrophy of the optic nerve, amaurosis, Descemet spots, cells in the aqueous humour, preretinal opacities of the vitreous body), intussusception of retinal vessels, grey infiltration of the retina.", "PMID": 957568}
{"id": "pubmed23n0032_2811", "title": "[Therapy of recurring herpetic corneal lesions with Vidarabin (author's transl)].", "content": "Vidarabin (Dr. Thilo) is an effective virostatic agent in recurring herpetic corneal lesions.", "contents": "[Therapy of recurring herpetic corneal lesions with Vidarabin (author's transl)]. Vidarabin (Dr. Thilo) is an effective virostatic agent in recurring herpetic corneal lesions.", "PMID": 957569}
{"id": "pubmed23n0032_2812", "title": "[Malignancies in relatives of retinoblastoma patients (author's transl)].", "content": "In 14 out of 25 patients with retinoblastoma there was a family history of malignancies. Most of the malignant tumours had been carcinomas. In 2 families relatives died because of leukaemia; in these cases the tumour was a so-called diffuse retinoblastoma occurring in elder children. A third family with a retinoblastoma in one child and leukaemia in another is mentioned. This unusual coincidence of two tumourous conditions of which one might be of viral origin led to detailed electron microscopic examination of the retinoblastoma in one case; but there were no signs of reduplicating viruses.", "contents": "[Malignancies in relatives of retinoblastoma patients (author's transl)]. In 14 out of 25 patients with retinoblastoma there was a family history of malignancies. Most of the malignant tumours had been carcinomas. In 2 families relatives died because of leukaemia; in these cases the tumour was a so-called diffuse retinoblastoma occurring in elder children. A third family with a retinoblastoma in one child and leukaemia in another is mentioned. This unusual coincidence of two tumourous conditions of which one might be of viral origin led to detailed electron microscopic examination of the retinoblastoma in one case; but there were no signs of reduplicating viruses.", "PMID": 957570}
{"id": "pubmed23n0032_2813", "title": "[Corneal dermoid cysts with other abnormalities (author's transl)].", "content": "Out of 22 patients with corneal dermoids, 9 showed other abnormalities consistent with the 1st branchial arch syndrome. In 4 cases there was hare lip, divided jaw and /or cleft palate along-side other abnormalities which made impossible a sure classification into one of the recognised syndromes. It is assumed that the anomalies arise in the 5th and 6th intrauterine week. The corneal dermoids were always unilateral and in only one case confined in position to the cornea.", "contents": "[Corneal dermoid cysts with other abnormalities (author's transl)]. Out of 22 patients with corneal dermoids, 9 showed other abnormalities consistent with the 1st branchial arch syndrome. In 4 cases there was hare lip, divided jaw and /or cleft palate along-side other abnormalities which made impossible a sure classification into one of the recognised syndromes. It is assumed that the anomalies arise in the 5th and 6th intrauterine week. The corneal dermoids were always unilateral and in only one case confined in position to the cornea.", "PMID": 957571}
{"id": "pubmed23n0032_2814", "title": "[Intravitreous cyst probably arising from ciliary body epithelium (author's transl)].", "content": "A description is given of a pigmented cyst situated within the anterior part of the vitreous body in the otherwise healthy eye of a girl aged 9 years.", "contents": "[Intravitreous cyst probably arising from ciliary body epithelium (author's transl)]. A description is given of a pigmented cyst situated within the anterior part of the vitreous body in the otherwise healthy eye of a girl aged 9 years.", "PMID": 957572}
{"id": "pubmed23n0032_2815", "title": "[Operative refractive correction of keratoconus with a modified Passow cautery apparatus (author's transl)].", "content": "The thermokeratoplasty was developed by Gasset for treatment of keratoconus. The flattening of the cornea is induced by heat. Indications and contra-indications are discussed. This is a report on our first 10 cases which were treated with a modified Passow-cauter. The procedure is simple and harmless and it seems to restrict the range of indications for corneal transplantation.", "contents": "[Operative refractive correction of keratoconus with a modified Passow cautery apparatus (author's transl)]. The thermokeratoplasty was developed by Gasset for treatment of keratoconus. The flattening of the cornea is induced by heat. Indications and contra-indications are discussed. This is a report on our first 10 cases which were treated with a modified Passow-cauter. The procedure is simple and harmless and it seems to restrict the range of indications for corneal transplantation.", "PMID": 957573}
{"id": "pubmed23n0032_2816", "title": "[Late choroidal detachment after i.c. cryoextraction of the lens (author's transl)].", "content": "Eighty days after i.c. cryoextraction of the lens without any complications, intra- or postoperative, choroidal detachment was observed in a 68-year-old woman (hypertension, diabetes m..amaurosis of the right eye after central artery embolism). -Conventional decongestive drugs (Reparil, Tantum, Tanderil) had no effect. Within 6 days of treatment with microwaves, 2.5% Hydrocortisone and atropine eye-drops and Prednisolone (10 mg/die) internally there was complete regression of the detachment.", "contents": "[Late choroidal detachment after i.c. cryoextraction of the lens (author's transl)]. Eighty days after i.c. cryoextraction of the lens without any complications, intra- or postoperative, choroidal detachment was observed in a 68-year-old woman (hypertension, diabetes m..amaurosis of the right eye after central artery embolism). -Conventional decongestive drugs (Reparil, Tantum, Tanderil) had no effect. Within 6 days of treatment with microwaves, 2.5% Hydrocortisone and atropine eye-drops and Prednisolone (10 mg/die) internally there was complete regression of the detachment.", "PMID": 957574}
{"id": "pubmed23n0032_2817", "title": "[Low-vision aids in visually handicapped children (author's transl)].", "content": "It is reported on 71 visually handicapped pupils. The visual acuity of 48 of these children could be tested with low-vision aids and the electronic television reading apparatus (Reinecker). With telescopic glasses (Zeiss, Keeler) 31 children had an average improvement of their visual acuity of two lines on a test board for near vision (Birkh\u00e4user). With the reading apparatus 43 children showed an improvement of the visual acuity of 5 more lines. Only 3 to 21 pupils, who had been offered a regular use of the telescopic glasses, decided to have one. Finally our own experiences are compared with those of other authors.", "contents": "[Low-vision aids in visually handicapped children (author's transl)]. It is reported on 71 visually handicapped pupils. The visual acuity of 48 of these children could be tested with low-vision aids and the electronic television reading apparatus (Reinecker). With telescopic glasses (Zeiss, Keeler) 31 children had an average improvement of their visual acuity of two lines on a test board for near vision (Birkh\u00e4user). With the reading apparatus 43 children showed an improvement of the visual acuity of 5 more lines. Only 3 to 21 pupils, who had been offered a regular use of the telescopic glasses, decided to have one. Finally our own experiences are compared with those of other authors.", "PMID": 957575}
{"id": "pubmed23n0032_2818", "title": "[A contribution to the pseudo-LE-syndrome. Investigations of the cardiovascular system, abdominal organs, lymphatic system and drug histories (author's transl)].", "content": "13 of 30 patients suffering from pseudo-LE-syndrome showed a usually reversible enlargement of the heart during the acute stage of the disease. In two patients carditis occurring in pseudo-LE-syndrome lead to congestive heart failure and an additional patient died in the acute stage of carditis. As opposed to Systemic Lupus Erythematosus hypertension did not occur in a higher frequency than normal. In five cases cardiac catheter examinations showed slight elevation of the end-diastolic pressure in the right ventricle, in two cases an abnormal high mean pressure in the right atrium and a systolic gradient at the pulmonic valve was found. -Scintigrams showed definite enlargement of the spleen and to a lesser degree enlargement of the liver was seen. Laparascopy showed multiple concretions after peritonitis. Lymphographic changes in the retroperitoneal lymph nodes and lymphatic ducts were not observed in contrast to rheumatic diseases. Drug histories in most cases discovered intake of Venopyronum dragees prior to onset of the disease. But recurrent attacks of the disease also occurred without further intake of the drug.", "contents": "[A contribution to the pseudo-LE-syndrome. Investigations of the cardiovascular system, abdominal organs, lymphatic system and drug histories (author's transl)]. 13 of 30 patients suffering from pseudo-LE-syndrome showed a usually reversible enlargement of the heart during the acute stage of the disease. In two patients carditis occurring in pseudo-LE-syndrome lead to congestive heart failure and an additional patient died in the acute stage of carditis. As opposed to Systemic Lupus Erythematosus hypertension did not occur in a higher frequency than normal. In five cases cardiac catheter examinations showed slight elevation of the end-diastolic pressure in the right ventricle, in two cases an abnormal high mean pressure in the right atrium and a systolic gradient at the pulmonic valve was found. -Scintigrams showed definite enlargement of the spleen and to a lesser degree enlargement of the liver was seen. Laparascopy showed multiple concretions after peritonitis. Lymphographic changes in the retroperitoneal lymph nodes and lymphatic ducts were not observed in contrast to rheumatic diseases. Drug histories in most cases discovered intake of Venopyronum dragees prior to onset of the disease. But recurrent attacks of the disease also occurred without further intake of the drug.", "PMID": 957598}
{"id": "pubmed23n0032_2819", "title": "Renin, aldosterone and arterial pressure responses to acute beta-adrenergic receptor blockage in hypertensive patients.", "content": "The effect of acute (intravenous) beta-adrenergic blockade with propranolol or pindolol on arterial pressure (BP), plasma renin activity (PRA), and plasma concentration of aldosterone (PA) was evaluated in 20 essential hypertensive men. BP, PRA and PA were determined during continuous recumbency over-night (8 p.m. to 6 a.m.) every 30 min. Two groups of patients were observed. Patients of group 1 exhibited a characteristic day-night rhythm of PRA with low values before midnight and large increases early in the morning. Conversely, no rhythm and very low PRA values were observed in patients of group II. BP was higher in group II than in group I. In group I following intravenous propranolol or pindolol, BP fell within minutes and levels as well as rhythms of PRA were converted to those of group II without treatment. In group II day-night profiles of PRA and BP remained unchanged. Rhythm and concentration of PA in the two groups were not influenced by either drug. In 4 patients of group I infusion of angiotensin II inhibitor did not lower BP. The observations suggest that in the two groups dissimilarities in rhythms of PRA as well as in BP responses to beta-blockade may reflect differences in neuro-adrenergic tone.", "contents": "Renin, aldosterone and arterial pressure responses to acute beta-adrenergic receptor blockage in hypertensive patients. The effect of acute (intravenous) beta-adrenergic blockade with propranolol or pindolol on arterial pressure (BP), plasma renin activity (PRA), and plasma concentration of aldosterone (PA) was evaluated in 20 essential hypertensive men. BP, PRA and PA were determined during continuous recumbency over-night (8 p.m. to 6 a.m.) every 30 min. Two groups of patients were observed. Patients of group 1 exhibited a characteristic day-night rhythm of PRA with low values before midnight and large increases early in the morning. Conversely, no rhythm and very low PRA values were observed in patients of group II. BP was higher in group II than in group I. In group I following intravenous propranolol or pindolol, BP fell within minutes and levels as well as rhythms of PRA were converted to those of group II without treatment. In group II day-night profiles of PRA and BP remained unchanged. Rhythm and concentration of PA in the two groups were not influenced by either drug. In 4 patients of group I infusion of angiotensin II inhibitor did not lower BP. The observations suggest that in the two groups dissimilarities in rhythms of PRA as well as in BP responses to beta-blockade may reflect differences in neuro-adrenergic tone.", "PMID": 957599}
{"id": "pubmed23n0032_2820", "title": "Influence of dehydrocholate sodium on the biliary reabsorption of sulfobromophtalein sodium from the rat biliary tree after retrograde intrabilary injection.", "content": "Bilary re-excretion of sulfobromophthalein sodium (BSP) (1.2 mumol/rat) after retrograde intrabilary injection is markedly inhibited by dehydrocholate sodium (Decholin) given intravenously as a constant infusion (3.1 mumol/min/kg body weight) or as a bolus injection (24.8 mumol/rat) to rats. Most interestingly Decholin demonstrates the same inhibitory effects on the biliary re-excretion of BSP when it is administered by retrograde intrabiliary injection (24.8 mumol/rat) together with the dye. In contrast to the inhibition of biliary re-excretion of BSP its biliary reabsorption from the biliary tract seems to be rather increased: about 80-85% of 1.2 mumol BSP are reabsorbed in all sets of experiments with Decholin in comparison to about 65% to the control group. Combined with histological data it is suggested that reabosorption of BSP after retrograde intrabiliary injection occurs at the ductular site whereas re-excretion takes place at the cannalicular membrane of the hepatocyte.", "contents": "Influence of dehydrocholate sodium on the biliary reabsorption of sulfobromophtalein sodium from the rat biliary tree after retrograde intrabilary injection. Bilary re-excretion of sulfobromophthalein sodium (BSP) (1.2 mumol/rat) after retrograde intrabilary injection is markedly inhibited by dehydrocholate sodium (Decholin) given intravenously as a constant infusion (3.1 mumol/min/kg body weight) or as a bolus injection (24.8 mumol/rat) to rats. Most interestingly Decholin demonstrates the same inhibitory effects on the biliary re-excretion of BSP when it is administered by retrograde intrabiliary injection (24.8 mumol/rat) together with the dye. In contrast to the inhibition of biliary re-excretion of BSP its biliary reabsorption from the biliary tract seems to be rather increased: about 80-85% of 1.2 mumol BSP are reabsorbed in all sets of experiments with Decholin in comparison to about 65% to the control group. Combined with histological data it is suggested that reabosorption of BSP after retrograde intrabiliary injection occurs at the ductular site whereas re-excretion takes place at the cannalicular membrane of the hepatocyte.", "PMID": 957600}
{"id": "pubmed23n0032_2821", "title": "Ultrastructural localization of concanavalin A in the perfused rat heart.", "content": "Staining of the surface coat of heart capillaries and aorta was achieved by perfusing concanavalin A and phytohemagglutinin coupled to horseradish peroxidase through the rat heart. The binding of these lectins provides evidence for the presence of glycoproteins containing mannose, glucose, and N-acetylgalactosamine residues in the endothelial surface coat of rat aorta and heart capillaries. The binding of concanavalin A to capillary endothelium was further followed with radioautography of 125I-labeled concanavalin A in the in vitro perfused rat heart. Following the shorter time intervals of perfusion more than 80 per cent of labeled concanavalin A was releasable by alpha-methyl-D-mannoside and the radioautographic reaction was confined to the endothelial cell surface. Following 60 minutes of perfusion with concanavalin A there was translocation of part of the lectin to the extravascular space and evidence of endothelial cell swelling. A tentative estimate of the concanavalin A binding sites in capillary endothelium was determined to be approximately 5000 per sq. mum.", "contents": "Ultrastructural localization of concanavalin A in the perfused rat heart. Staining of the surface coat of heart capillaries and aorta was achieved by perfusing concanavalin A and phytohemagglutinin coupled to horseradish peroxidase through the rat heart. The binding of these lectins provides evidence for the presence of glycoproteins containing mannose, glucose, and N-acetylgalactosamine residues in the endothelial surface coat of rat aorta and heart capillaries. The binding of concanavalin A to capillary endothelium was further followed with radioautography of 125I-labeled concanavalin A in the in vitro perfused rat heart. Following the shorter time intervals of perfusion more than 80 per cent of labeled concanavalin A was releasable by alpha-methyl-D-mannoside and the radioautographic reaction was confined to the endothelial cell surface. Following 60 minutes of perfusion with concanavalin A there was translocation of part of the lectin to the extravascular space and evidence of endothelial cell swelling. A tentative estimate of the concanavalin A binding sites in capillary endothelium was determined to be approximately 5000 per sq. mum.", "PMID": 957601}
{"id": "pubmed23n0032_2822", "title": "Lysosomal alterations in cultured macrophages exposed to anorexigenic and psychotropic drugs.", "content": "Cultured rat macrophages were used for an in vitro study of drug-induced lipidosis. Cells were exposed for 24 hours to equimolar concentrations (5 X 10(-5) and 1 X 10(-4) M) of the following amphiphilic (amphipathic) cationic drugs: chlorphentermine, amitriptyline, 1-chloro-amitriptyline, iprindole, noxiptiline, chlorpromazine. In addition the less amphiphilic drug phentermine was used. Ultrastructurally, the cytologic changes essentially consisted of formation of multilamellated cytoplasmic inclusions, which possessed acid phosphatase activity. The abnormal inclusions are interpreted to result from intralysosomal accumulation of polar lipids. Under the present in vitro conditions all drugs except phentermine, had similar potencies to induce such lysosomal alterations, quite in contrast to the great quantitative differences previously observed under in vivo conditions. The present results lend further support to a concept that regards a pronounced amphiphilic (amphipathic) character to be responsible for the lipidosis-inducing action of various cationic compounds. Cultured macrophages are suggested as a useful tool to investigate this structure-activity relationship, which under in vivo conditions may be obscured by superimposed parameters such as drug metabolism.", "contents": "Lysosomal alterations in cultured macrophages exposed to anorexigenic and psychotropic drugs. Cultured rat macrophages were used for an in vitro study of drug-induced lipidosis. Cells were exposed for 24 hours to equimolar concentrations (5 X 10(-5) and 1 X 10(-4) M) of the following amphiphilic (amphipathic) cationic drugs: chlorphentermine, amitriptyline, 1-chloro-amitriptyline, iprindole, noxiptiline, chlorpromazine. In addition the less amphiphilic drug phentermine was used. Ultrastructurally, the cytologic changes essentially consisted of formation of multilamellated cytoplasmic inclusions, which possessed acid phosphatase activity. The abnormal inclusions are interpreted to result from intralysosomal accumulation of polar lipids. Under the present in vitro conditions all drugs except phentermine, had similar potencies to induce such lysosomal alterations, quite in contrast to the great quantitative differences previously observed under in vivo conditions. The present results lend further support to a concept that regards a pronounced amphiphilic (amphipathic) character to be responsible for the lipidosis-inducing action of various cationic compounds. Cultured macrophages are suggested as a useful tool to investigate this structure-activity relationship, which under in vivo conditions may be obscured by superimposed parameters such as drug metabolism.", "PMID": 957602}
{"id": "pubmed23n0032_2823", "title": "Ultrastructural alterations of rat lung exposed to pyrolysis products of polytetrafluoroethylene (PTFE, Teflon).", "content": "Ultrastructural changes of the lung tissues were evaluated in rats exposed to the pyrolysis products of Teflon at 380 degrees C., 400 degrees C;, and 450 degrees C., respectively, for 4 hours. At 450 degrees C. Teflon evolved numerous tiny particles (0.02 to 0.04 mum.), which were considered to be the toxic material, and at 550 degrees C., in addition to the tiny particles, large spherical particles (0.7 to 5.0 mum) were found. No particles were observed in the pyrolysis product produced at temperatures between 380 degrees C. and 425 degrees C. There were no clinical symptoms or lung alterations induced at temperatures below 425 degrees C colloidal carbon injected as a marker of increased vascular permeability did not pass through the alveolar capillary endothelium. At 450 degrees C. (approximate lethal temperature) rats revealed severe respiratory difficulty, pulmonary edema, hemorrhage, and necrosis of the tracheobronchial epithelium. The membranous pneumocytes appear to be vulnerable to the Teflon fume causing cytoplasmic swelling, bleb formation, fragmentation, and denudation. Similar changes were found in the endothelial cells, but to a much lesser extent. When Teflon fume generated at 450 degrees CnWAS FILTERED THROUGH A Millipore filter (pore size 0.2 mum.), rats revealed only slight degenerative changes in the superficial tracheobronchial epithelium, but no clinical signs.", "contents": "Ultrastructural alterations of rat lung exposed to pyrolysis products of polytetrafluoroethylene (PTFE, Teflon). Ultrastructural changes of the lung tissues were evaluated in rats exposed to the pyrolysis products of Teflon at 380 degrees C., 400 degrees C;, and 450 degrees C., respectively, for 4 hours. At 450 degrees C. Teflon evolved numerous tiny particles (0.02 to 0.04 mum.), which were considered to be the toxic material, and at 550 degrees C., in addition to the tiny particles, large spherical particles (0.7 to 5.0 mum) were found. No particles were observed in the pyrolysis product produced at temperatures between 380 degrees C. and 425 degrees C. There were no clinical symptoms or lung alterations induced at temperatures below 425 degrees C colloidal carbon injected as a marker of increased vascular permeability did not pass through the alveolar capillary endothelium. At 450 degrees C. (approximate lethal temperature) rats revealed severe respiratory difficulty, pulmonary edema, hemorrhage, and necrosis of the tracheobronchial epithelium. The membranous pneumocytes appear to be vulnerable to the Teflon fume causing cytoplasmic swelling, bleb formation, fragmentation, and denudation. Similar changes were found in the endothelial cells, but to a much lesser extent. When Teflon fume generated at 450 degrees CnWAS FILTERED THROUGH A Millipore filter (pore size 0.2 mum.), rats revealed only slight degenerative changes in the superficial tracheobronchial epithelium, but no clinical signs.", "PMID": 957603}
{"id": "pubmed23n0032_2824", "title": "Ultrastructural differences between rat inner medullary descending and ascending vasa recta;.", "content": "The ultrastructure of rat inner medullary vasa recta was studied by both conventional transmission and freeze-fracture electron microscopy. The identify of descending and ascending vasa recta in the inner medulla was established by tracing outer medullary descending vasa recta and ascending vasa recta into the inner medulla, as well as by the incomplete carbon labeling technique to identify isolated descending vessels or loops. As in the outer medulla, descending vessels possess thick continuous endothelium with pinocytotic vesicles budding off the luminal and basal plasma membranes (more numerous in the latter location), but no fenestrae. Ascending vasa show thin, attenuated endothelium with numerous (500 to 800 A fenestrae bridged by 40 A thick fenestral diaphragms. Intercellular junctions in both vessels are zonulae occludens with usually one, and uncommonly two or three zones of outer leaflet membrane fusion. Intramembranous particles are numerous in endothelial plasma membranes of descending vasa recta; similar particles are much fewer in corresponding ascending vasa recta endothelial plasma membranes. The ultrastructural differences between descending vasa recta and ascending vasa recta may reflect markedly different permeability properties, particularly to macromolecules, and may be relevant to recent functional studies on rat inner medullary vasa recta.", "contents": "Ultrastructural differences between rat inner medullary descending and ascending vasa recta;. The ultrastructure of rat inner medullary vasa recta was studied by both conventional transmission and freeze-fracture electron microscopy. The identify of descending and ascending vasa recta in the inner medulla was established by tracing outer medullary descending vasa recta and ascending vasa recta into the inner medulla, as well as by the incomplete carbon labeling technique to identify isolated descending vessels or loops. As in the outer medulla, descending vessels possess thick continuous endothelium with pinocytotic vesicles budding off the luminal and basal plasma membranes (more numerous in the latter location), but no fenestrae. Ascending vasa show thin, attenuated endothelium with numerous (500 to 800 A fenestrae bridged by 40 A thick fenestral diaphragms. Intercellular junctions in both vessels are zonulae occludens with usually one, and uncommonly two or three zones of outer leaflet membrane fusion. Intramembranous particles are numerous in endothelial plasma membranes of descending vasa recta; similar particles are much fewer in corresponding ascending vasa recta endothelial plasma membranes. The ultrastructural differences between descending vasa recta and ascending vasa recta may reflect markedly different permeability properties, particularly to macromolecules, and may be relevant to recent functional studies on rat inner medullary vasa recta.", "PMID": 957604}
{"id": "pubmed23n0032_2825", "title": "Injury of the cell's respiratory system by heat and by formaldehyde. Thermokinetics and early molecular events.", "content": "This is a study of the manner in which the respiratory system of the cell is injured either by elevated temperature or by exposure to diluted formaldehyde. Molecular mechanisms were identified by thermokinetic measurements. The rates at which respiratory failure developed in mouse liver slices in an injurious environment were measured at various temperatures. The data were fitted to the Arrhenius equation, and the effective activation energies of the injury processes were calculated. These data show that (1) the thermokinetics of injury to the cell's respiratory system, whether by thermal or chemical means, follows the Arrhenius law. (2) Thermal injury of the cell's respiratory system has a high activation energy, indicating that the critical, rate-determining event is a protein denaturation. Other mechanisms such as imbalance of metabolic reaction rates and thermal liquefaction of membrane lipids can be ruled out. (3) Repression of cell respiration by diluted formaldehyde has an activation energy compatible with a chemical reaction but low enough to exclude protein denaturation as a mechanism.", "contents": "Injury of the cell's respiratory system by heat and by formaldehyde. Thermokinetics and early molecular events. This is a study of the manner in which the respiratory system of the cell is injured either by elevated temperature or by exposure to diluted formaldehyde. Molecular mechanisms were identified by thermokinetic measurements. The rates at which respiratory failure developed in mouse liver slices in an injurious environment were measured at various temperatures. The data were fitted to the Arrhenius equation, and the effective activation energies of the injury processes were calculated. These data show that (1) the thermokinetics of injury to the cell's respiratory system, whether by thermal or chemical means, follows the Arrhenius law. (2) Thermal injury of the cell's respiratory system has a high activation energy, indicating that the critical, rate-determining event is a protein denaturation. Other mechanisms such as imbalance of metabolic reaction rates and thermal liquefaction of membrane lipids can be ruled out. (3) Repression of cell respiration by diluted formaldehyde has an activation energy compatible with a chemical reaction but low enough to exclude protein denaturation as a mechanism.", "PMID": 957605}
{"id": "pubmed23n0032_2826", "title": "Ultrastructural and morphometric studies of platelets from cattle with the Chediak-Higashi syndrome.", "content": "This study revealed that the lesions in platelets from cattle with the Chediak-Higashi syndrome (CHS) are not morphologically analogousto those present in the other granule-containing cells in cattle and other species with CHS.  There was no evidence of enlargement of the lysosomal granules in bovine CHS platelets...", "contents": "Ultrastructural and morphometric studies of platelets from cattle with the Chediak-Higashi syndrome. This study revealed that the lesions in platelets from cattle with the Chediak-Higashi syndrome (CHS) are not morphologically analogousto those present in the other granule-containing cells in cattle and other species with CHS.  There was no evidence of enlargement of the lysosomal granules in bovine CHS platelets...", "PMID": 957606}
{"id": "pubmed23n0032_2827", "title": "Renewal of the terminal bronchiolar epithelium in the rat following exposure to NO2 or O3.", "content": "Rats were exposed to either NO2 or O3 to determine whether nonciliated cells (Clara cells) could divide and differentiate into ciliated cells in the terminal bronchioles. Dividing cells were labeled with tritiated thymidine, visualized in the light and electron microscopes using autoradiographic techniques, and studied for up to 15 days after labeling. Electron microscopic autoradiography 1 hour after injection of tritiated thymidine showed that all labeled cells in the terminal bronchioles were nonciliated. However, 4 days after injection of tritiated thymidine, 67.8 per cent of the labeled cells were nonciliated and 32.2 per cent were ciliated. Light microscopic autoradiography showed that the new labeled ciliated cell population was stable for up to 15 days. These results indicate that nonciliated cells divide and the sister cells may form new ciliated and nonciliated cells. Thus, nonciliated cells can act as progenitor cells for the terminal bronchiolar epithelium.", "contents": "Renewal of the terminal bronchiolar epithelium in the rat following exposure to NO2 or O3. Rats were exposed to either NO2 or O3 to determine whether nonciliated cells (Clara cells) could divide and differentiate into ciliated cells in the terminal bronchioles. Dividing cells were labeled with tritiated thymidine, visualized in the light and electron microscopes using autoradiographic techniques, and studied for up to 15 days after labeling. Electron microscopic autoradiography 1 hour after injection of tritiated thymidine showed that all labeled cells in the terminal bronchioles were nonciliated. However, 4 days after injection of tritiated thymidine, 67.8 per cent of the labeled cells were nonciliated and 32.2 per cent were ciliated. Light microscopic autoradiography showed that the new labeled ciliated cell population was stable for up to 15 days. These results indicate that nonciliated cells divide and the sister cells may form new ciliated and nonciliated cells. Thus, nonciliated cells can act as progenitor cells for the terminal bronchiolar epithelium.", "PMID": 957607}
{"id": "pubmed23n0032_2828", "title": "Performance of an abdominal left ventricular assist device during induced tachycardias and dysrhythmias.", "content": "Experiments were designed to assess the performance of an intracorporeal (abdominal) left ventricular assist device (ALVAD) in the presence of induced tachycardias, multiple premature ventricular contractions (PVC's), and ventricular fibrillation in calves. Performance criteria were the degree of left ventricular unloading and the per cent cardiac output assumed by the ALVAD. During synchronous pumping, left ventricular unloading was complete and the entire cardiac output was captured by the device. During induced tachycardias up to rates of 120 beats per minute, these degrees of performance were maintained. At rates in excess of 120 beats per minute, performance declined due to decreased biologic stroke volumes and prosthetic filling times. In the presence of induced PVC's, performance during synchronous pumping decreased because of erratic R-wave sensing. Left ventricular unloading was complete but irregular, and the total cardiac output was captured. When asynchronous pumping was utilized, mean left ventricular systolic pressures increased, but total cardiac output was still captured. During induced ventricular fibrillation, ALVAD actuation maintained cardiac outputs equal to control values for periods up to 5 1/2 hours. These experiments indicate that, during normal sinus rhythm, synchronous pumping is optimal; asynchronous pumping is optimal during complex dysrhythmias; and either can be utilized to support the circulation with varying degrees of left ventricular unloading.", "contents": "Performance of an abdominal left ventricular assist device during induced tachycardias and dysrhythmias. Experiments were designed to assess the performance of an intracorporeal (abdominal) left ventricular assist device (ALVAD) in the presence of induced tachycardias, multiple premature ventricular contractions (PVC's), and ventricular fibrillation in calves. Performance criteria were the degree of left ventricular unloading and the per cent cardiac output assumed by the ALVAD. During synchronous pumping, left ventricular unloading was complete and the entire cardiac output was captured by the device. During induced tachycardias up to rates of 120 beats per minute, these degrees of performance were maintained. At rates in excess of 120 beats per minute, performance declined due to decreased biologic stroke volumes and prosthetic filling times. In the presence of induced PVC's, performance during synchronous pumping decreased because of erratic R-wave sensing. Left ventricular unloading was complete but irregular, and the total cardiac output was captured. When asynchronous pumping was utilized, mean left ventricular systolic pressures increased, but total cardiac output was still captured. During induced ventricular fibrillation, ALVAD actuation maintained cardiac outputs equal to control values for periods up to 5 1/2 hours. These experiments indicate that, during normal sinus rhythm, synchronous pumping is optimal; asynchronous pumping is optimal during complex dysrhythmias; and either can be utilized to support the circulation with varying degrees of left ventricular unloading.", "PMID": 957732}
{"id": "pubmed23n0032_2829", "title": "Congenital aortic stenosis. Experience with 43 patients.", "content": "Between September, 1967, and January, 1975, 43 patients underwent intracardiac repair for congenital aortic stenosis at the Buffalo Children's Hospital. The patients ranged in age from 2 days to 24 years, 6 of them being below one year of age. Valvular aortic stenosis was found in 21 cases (4 infants [Group I-A] and 17 older patients [Group I-B]), discrete subaortic membranous diaphragm in 11 (Group II); diffuse subvalvular muscular obstruction in 3 (Group III), supravalvular stenosis in 4 (Group IV), and multiple-level obstruction in 4 (2 infants [Group V-A] and 2 older patients [Group V-B]). Preoperatively, 58 per cent of the patients were symptomatic and 67 per cent had abnormal electrocardiograms. Associated congenital cardiac defects were found in 28 per cent of the cases. The over-all hospital mortality rate was 9 per cent (3 patients in Group I-A and one in Group V-A), with no deaths occurring in patients older than 3 months of age at the time of operation. Two late deaths occurred (Groups I-B and V-B). A complete heart block developed in one patient (Group III). The average intraoperative peak systolic left ventricular-aortic gradient decreased in all groups after repair but progressively increased in the late hemodynamic studies obtained in symptomatic patients. Six patients were reoperated upon for recurrent obstruction. Late results were evaluated on the basis of symptoms, electrocardiographic findings, valve function, and hemodynamic data. They showed excellent or satisfactory results in 59 per cent of the patients in Group I-B, in 45 per cent in Group II, in 66 per cent in Group III, and in 25 per cent in Group I-V. Results were fair or poor in Groups, I-A, V-A, and V-B. In children and adolescents, effective relief of the obstruction and of the symptoms can be obtained with minimal operative risk and minimal morbidity. In symptomatic infants, despite the high operative mortality rate, surgical intervention is indicated because of the poor prognosis.", "contents": "Congenital aortic stenosis. Experience with 43 patients. Between September, 1967, and January, 1975, 43 patients underwent intracardiac repair for congenital aortic stenosis at the Buffalo Children's Hospital. The patients ranged in age from 2 days to 24 years, 6 of them being below one year of age. Valvular aortic stenosis was found in 21 cases (4 infants [Group I-A] and 17 older patients [Group I-B]), discrete subaortic membranous diaphragm in 11 (Group II); diffuse subvalvular muscular obstruction in 3 (Group III), supravalvular stenosis in 4 (Group IV), and multiple-level obstruction in 4 (2 infants [Group V-A] and 2 older patients [Group V-B]). Preoperatively, 58 per cent of the patients were symptomatic and 67 per cent had abnormal electrocardiograms. Associated congenital cardiac defects were found in 28 per cent of the cases. The over-all hospital mortality rate was 9 per cent (3 patients in Group I-A and one in Group V-A), with no deaths occurring in patients older than 3 months of age at the time of operation. Two late deaths occurred (Groups I-B and V-B). A complete heart block developed in one patient (Group III). The average intraoperative peak systolic left ventricular-aortic gradient decreased in all groups after repair but progressively increased in the late hemodynamic studies obtained in symptomatic patients. Six patients were reoperated upon for recurrent obstruction. Late results were evaluated on the basis of symptoms, electrocardiographic findings, valve function, and hemodynamic data. They showed excellent or satisfactory results in 59 per cent of the patients in Group I-B, in 45 per cent in Group II, in 66 per cent in Group III, and in 25 per cent in Group I-V. Results were fair or poor in Groups, I-A, V-A, and V-B. In children and adolescents, effective relief of the obstruction and of the symptoms can be obtained with minimal operative risk and minimal morbidity. In symptomatic infants, despite the high operative mortality rate, surgical intervention is indicated because of the poor prognosis.", "PMID": 957733}
{"id": "pubmed23n0032_2830", "title": "The conduction system in simple, regular (D-), complete transposition with ventricular septal defect.", "content": "This is a serial section study of the conduction system in 5 cases of complete (d-) transposition with ventricular septal defect (VSD) . In 3 of the cases with infracristal or intracristal VSD, the bundle lay in the posterior (inferior) wall on the left side of the defect. In the fourth case with infracristal VSD it was on the right side of this wall. In the case with a more distally situated VSD, the bundle branches skirted the anterior and distal walls of the defect. This study shows that in closure of the VSD in complete transposition, the posterior wall of the defect in some cases, and in others the anterior and distal walls of the defect are to be handled judiciously. The right side of this wall is probably safer to handle from the standpoint of block than the left side in most cases. His bundle recording during open-heart surgery may be indicated in large or unusually located VSD's to delineate the course of the conduction system and thereby to avoid atrioventricular (A-V) block.", "contents": "The conduction system in simple, regular (D-), complete transposition with ventricular septal defect. This is a serial section study of the conduction system in 5 cases of complete (d-) transposition with ventricular septal defect (VSD) . In 3 of the cases with infracristal or intracristal VSD, the bundle lay in the posterior (inferior) wall on the left side of the defect. In the fourth case with infracristal VSD it was on the right side of this wall. In the case with a more distally situated VSD, the bundle branches skirted the anterior and distal walls of the defect. This study shows that in closure of the VSD in complete transposition, the posterior wall of the defect in some cases, and in others the anterior and distal walls of the defect are to be handled judiciously. The right side of this wall is probably safer to handle from the standpoint of block than the left side in most cases. His bundle recording during open-heart surgery may be indicated in large or unusually located VSD's to delineate the course of the conduction system and thereby to avoid atrioventricular (A-V) block.", "PMID": 957734}
{"id": "pubmed23n0032_2831", "title": "Evaluating techniques of myocardial preservation for aortic valve replacement: operative risk.", "content": "In a two-year period 105 patients underwent isolated aortic valve replacement without hospital death. Eighty-three patients were operated on using hypothermic ischemic arrest; 22 patients had coronary perfusion. The early postoperative cardiac performance and incidence of complications did not differ between groups, although cardiopulmonary bypass time was shorter in the ischemic arrest group. These data suggest that ischemic arrest is a satisfactory method for myocardial preservation. However, because of the low hospital mortality rate with either method, these results and those of others cannot be taken to indicate the superiority of any method of myocardial preservation.", "contents": "Evaluating techniques of myocardial preservation for aortic valve replacement: operative risk. In a two-year period 105 patients underwent isolated aortic valve replacement without hospital death. Eighty-three patients were operated on using hypothermic ischemic arrest; 22 patients had coronary perfusion. The early postoperative cardiac performance and incidence of complications did not differ between groups, although cardiopulmonary bypass time was shorter in the ischemic arrest group. These data suggest that ischemic arrest is a satisfactory method for myocardial preservation. However, because of the low hospital mortality rate with either method, these results and those of others cannot be taken to indicate the superiority of any method of myocardial preservation.", "PMID": 957735}
{"id": "pubmed23n0032_2832", "title": "A different approach for the total correction of tricuspid atresia.", "content": "A new surgical procedure has been used successfully to correct tricuspid atresia in a 9-year-old girl. An external conduit containing a porcine aortic valve was positioned to lead from the right atrium to the underdeveloped right ventricle. The right ventricle was reconstructed with a large Dacron patch, thus providing a large cavity for the small hypoplastic right ventricle. The atrial and ventricular septal defects were closed. The patient made a satisfactory recovery and is doing well four months after the operation.", "contents": "A different approach for the total correction of tricuspid atresia. A new surgical procedure has been used successfully to correct tricuspid atresia in a 9-year-old girl. An external conduit containing a porcine aortic valve was positioned to lead from the right atrium to the underdeveloped right ventricle. The right ventricle was reconstructed with a large Dacron patch, thus providing a large cavity for the small hypoplastic right ventricle. The atrial and ventricular septal defects were closed. The patient made a satisfactory recovery and is doing well four months after the operation.", "PMID": 957736}
{"id": "pubmed23n0032_2833", "title": "Complete repair of transposition of the great arteries with pulmonary atresia.", "content": "In this series of 13 patients undergoing repair of transposition of the great arteries with ventricular septal defect and pulmonary atresia, the operative risk and postoperative complications were greater than for repair of either transposition of the great arteries with ventricular septal defect and pulmonary stenosis or pulmonary arterial atresia with ventricular defect. Nevertheless, 6 of the 8 survivors improved clinically. Because operative and late mortality and morbidity rates are related to persistent right ventricular hypertension caused primarily by restricted pulmonary arterial outflow, results should be improved by performance of a preliminary systemic-pulmonary artery shunt for patients with hypoplastic pulmonary arteries and by use of the Hancock prosthesis, which has yielded lower gradients at both the proximal and distal anastomoses of the conduit. These improvements and the relief from cyanosis, exercise intolerance, and other symptoms seem to justify the continued application of surgical correction of transposition of the great arteries when associated with pulmonary atresia.", "contents": "Complete repair of transposition of the great arteries with pulmonary atresia. In this series of 13 patients undergoing repair of transposition of the great arteries with ventricular septal defect and pulmonary atresia, the operative risk and postoperative complications were greater than for repair of either transposition of the great arteries with ventricular septal defect and pulmonary stenosis or pulmonary arterial atresia with ventricular defect. Nevertheless, 6 of the 8 survivors improved clinically. Because operative and late mortality and morbidity rates are related to persistent right ventricular hypertension caused primarily by restricted pulmonary arterial outflow, results should be improved by performance of a preliminary systemic-pulmonary artery shunt for patients with hypoplastic pulmonary arteries and by use of the Hancock prosthesis, which has yielded lower gradients at both the proximal and distal anastomoses of the conduit. These improvements and the relief from cyanosis, exercise intolerance, and other symptoms seem to justify the continued application of surgical correction of transposition of the great arteries when associated with pulmonary atresia.", "PMID": 957737}
{"id": "pubmed23n0032_2834", "title": "Ventricular pacing with epigastric transdiaphragmatic electrodes. Long-term follow-up and comparison with other implantation methods.", "content": "One hundred eighty-seven electrode systems were implanted in 158 consecutive patients--30 systems implanted by thoracotomy, 98 transvenous, and 52 epigastric transdiaphragmatic systems--and the long-term results were compared. With electrodes placed by thoracotomy there was a high rate of complication (76 per cent), prolonging the initial hospitalization (33 per cent) and necessitating frequent rehospitalizations (56 per cent). With transvenous electrodes the complication rate was 39 per cent (16 per cent during the first hospitalization, 28 per cent necessitating rehospitalization). With epigastric transdiaphragmatic electrodes these figures were, respectively, 15, 12, and 6 per cent. The most frequent complications with transvenous electrodes were displacement (15 per cent), ulcerations, and local infection; all were abolished when the epigastric approach was used. Threshold elevation was not more frequent with epigastric electrodes (sutured to the right ventricle) than with the other techniques. Four per cent of the epigastric electrodes caused complications severe enough to require another mode of pacing, contrasting with 18 and 26 per cent, respectively, for the transvenous and transthoracic electrodes.", "contents": "Ventricular pacing with epigastric transdiaphragmatic electrodes. Long-term follow-up and comparison with other implantation methods. One hundred eighty-seven electrode systems were implanted in 158 consecutive patients--30 systems implanted by thoracotomy, 98 transvenous, and 52 epigastric transdiaphragmatic systems--and the long-term results were compared. With electrodes placed by thoracotomy there was a high rate of complication (76 per cent), prolonging the initial hospitalization (33 per cent) and necessitating frequent rehospitalizations (56 per cent). With transvenous electrodes the complication rate was 39 per cent (16 per cent during the first hospitalization, 28 per cent necessitating rehospitalization). With epigastric transdiaphragmatic electrodes these figures were, respectively, 15, 12, and 6 per cent. The most frequent complications with transvenous electrodes were displacement (15 per cent), ulcerations, and local infection; all were abolished when the epigastric approach was used. Threshold elevation was not more frequent with epigastric electrodes (sutured to the right ventricle) than with the other techniques. Four per cent of the epigastric electrodes caused complications severe enough to require another mode of pacing, contrasting with 18 and 26 per cent, respectively, for the transvenous and transthoracic electrodes.", "PMID": 957738}
{"id": "pubmed23n0032_2835", "title": "A safe technique for closure of the recurrent patent ductus arteriosus.", "content": "In an early series of 73 operations for patent ductus arteriosus, closure was by the triple ligation method. Over a short period, recurrence of patency was observed in 2 cases and after that the technique of division and suture rather than ligation became standard. For closure of the recurrent ductus, a modification of the usual technique is necessary to minimize danger of injury to the recurrent nerve and the great vessels. A safe method was devised which involves an intrapericardial approach to the superior aspect of the ductus and dissection inferiorly which avoids denudation of the aorta. This technique was used with satisfaction in 6 cases of recurrent patent ductus arteriosus.", "contents": "A safe technique for closure of the recurrent patent ductus arteriosus. In an early series of 73 operations for patent ductus arteriosus, closure was by the triple ligation method. Over a short period, recurrence of patency was observed in 2 cases and after that the technique of division and suture rather than ligation became standard. For closure of the recurrent ductus, a modification of the usual technique is necessary to minimize danger of injury to the recurrent nerve and the great vessels. A safe method was devised which involves an intrapericardial approach to the superior aspect of the ductus and dissection inferiorly which avoids denudation of the aorta. This technique was used with satisfaction in 6 cases of recurrent patent ductus arteriosus.", "PMID": 957739}
{"id": "pubmed23n0032_2836", "title": "Definitive surgical treatment of anomalous origin of left coronary artery. A new technical approach used successfully in a seven-month-old male infant.", "content": "Anomalous origin of left coronary artery from the pulmonary artery is a very rare cardiac anomaly. Most patients with this defect die in the first year of life. Reconstructive surgery of the left coronary artery is the only saving measure in the management of this disease. A radical operation was successfully carried out in a seven-month-old boy by interposing a homologous saphenous vein (from his mother) between the aorta and left coronary artery. The operation was performed by use of deep hypothermia with surface cooling combined with limited cardiopulmonary bypass. The postoperative clinical course was uneventful and the patient relatively asymptomatic. Postoperative coronary angiograms revealed that the interposed homologous vein graft was patent, although it was slightly stenotic. Following the operation the patient showed good development and growth and has now reached 2 years of age. The purpose of this paper is to present the case by discussing our new operative technique.", "contents": "Definitive surgical treatment of anomalous origin of left coronary artery. A new technical approach used successfully in a seven-month-old male infant. Anomalous origin of left coronary artery from the pulmonary artery is a very rare cardiac anomaly. Most patients with this defect die in the first year of life. Reconstructive surgery of the left coronary artery is the only saving measure in the management of this disease. A radical operation was successfully carried out in a seven-month-old boy by interposing a homologous saphenous vein (from his mother) between the aorta and left coronary artery. The operation was performed by use of deep hypothermia with surface cooling combined with limited cardiopulmonary bypass. The postoperative clinical course was uneventful and the patient relatively asymptomatic. Postoperative coronary angiograms revealed that the interposed homologous vein graft was patent, although it was slightly stenotic. Following the operation the patient showed good development and growth and has now reached 2 years of age. The purpose of this paper is to present the case by discussing our new operative technique.", "PMID": 957740}
{"id": "pubmed23n0032_2837", "title": "A low-threshold, non-dislocating endocardial electrode.", "content": "Threshold curves with large and small surface intracardiac pacemaker electrodes are compared. The 2 msec. impulse threshold with a 47 sq. mm. electrode was 3.6 v. (4.3 mA.) on the fourteenth postoperative day, when it reached its maximum, and 2.8 v. (3.1 mA.) one month after the operation. These values were 45 and 30 per cent lower with a 6 sq. mm. electrode. Thresholds increased by about 20 per cent when the impulse duration was shortened from 2 to 0.5 msec. The small surface electrode consumed about 35 per cent less current than the 47 sq. mm. one. A newly designed large area-small surface electrode with the shape of an open cage, seems to have the advantages of less increase in postoperative thresholds and good attachment to the endocardial wall.", "contents": "A low-threshold, non-dislocating endocardial electrode. Threshold curves with large and small surface intracardiac pacemaker electrodes are compared. The 2 msec. impulse threshold with a 47 sq. mm. electrode was 3.6 v. (4.3 mA.) on the fourteenth postoperative day, when it reached its maximum, and 2.8 v. (3.1 mA.) one month after the operation. These values were 45 and 30 per cent lower with a 6 sq. mm. electrode. Thresholds increased by about 20 per cent when the impulse duration was shortened from 2 to 0.5 msec. The small surface electrode consumed about 35 per cent less current than the 47 sq. mm. one. A newly designed large area-small surface electrode with the shape of an open cage, seems to have the advantages of less increase in postoperative thresholds and good attachment to the endocardial wall.", "PMID": 957741}
{"id": "pubmed23n0032_2838", "title": "Infection of cardiac suture line after ventricular aneurysmectomy. Report of two cases.", "content": "Two cases of sepsis following ventricular aneurysmectomy are presented. In both, the source of sepsis was an infected caridac suture line in which Teflon felt strips were used to reinforce the closure. One patient had a pseudoaneurysm and a chronic empyema of the left side of the chest, and the second had a ventriculocutaneous fistula. Early operation with removal of the foreign body is warranted in this major complication of a ventricular aneurysmectomy.", "contents": "Infection of cardiac suture line after ventricular aneurysmectomy. Report of two cases. Two cases of sepsis following ventricular aneurysmectomy are presented. In both, the source of sepsis was an infected caridac suture line in which Teflon felt strips were used to reinforce the closure. One patient had a pseudoaneurysm and a chronic empyema of the left side of the chest, and the second had a ventriculocutaneous fistula. Early operation with removal of the foreign body is warranted in this major complication of a ventricular aneurysmectomy.", "PMID": 957742}
{"id": "pubmed23n0032_2839", "title": "Doppler ultrasonic flow detection as an adjunct in the diagnosis of patent ductus arteriosus in premature infants.", "content": "Since ligation of a patent ductus arteriosus in the neonate with idiopathic respiratory distress syndrome seems to improve the clinical condition of the infant, we have attempted to identify those neonates earlier with the noninvasive Doppler ultrasonic technique, recording brachial arterial flow signals. Sixteen patients were studied. In the 5 with reversed brachial flow who were operated upon, ligation of a PDA resulted in improvement and postoperative correction of brachial flow reversal as evidenced by the Doppler ultrasonic technique.", "contents": "Doppler ultrasonic flow detection as an adjunct in the diagnosis of patent ductus arteriosus in premature infants. Since ligation of a patent ductus arteriosus in the neonate with idiopathic respiratory distress syndrome seems to improve the clinical condition of the infant, we have attempted to identify those neonates earlier with the noninvasive Doppler ultrasonic technique, recording brachial arterial flow signals. Sixteen patients were studied. In the 5 with reversed brachial flow who were operated upon, ligation of a PDA resulted in improvement and postoperative correction of brachial flow reversal as evidenced by the Doppler ultrasonic technique.", "PMID": 957743}
{"id": "pubmed23n0032_2840", "title": "Total correction of double-outlet ventricle with concordant atrioventricular relation, dextrocardia, pulmonary stenosis, and situs inversus.", "content": "A case of successful surgical correction of double-outlet right ventricle with concordant atrioventricular relation, pulmonic stenosis, and situs inversus is described. Transposition of the great vessels was not present. Intracardiac repair with a Teflon tunnel conduit gave an excellent result. Proper resection of the subpulmonic obstructing fibromuscular band required no patch reconstruction of the outflow tract.", "contents": "Total correction of double-outlet ventricle with concordant atrioventricular relation, dextrocardia, pulmonary stenosis, and situs inversus. A case of successful surgical correction of double-outlet right ventricle with concordant atrioventricular relation, pulmonic stenosis, and situs inversus is described. Transposition of the great vessels was not present. Intracardiac repair with a Teflon tunnel conduit gave an excellent result. Proper resection of the subpulmonic obstructing fibromuscular band required no patch reconstruction of the outflow tract.", "PMID": 957744}
{"id": "pubmed23n0032_2841", "title": "Effects of assisted ventilation on echocardiographic findings in two infants with patent ductus arteriosus.", "content": "Echocardiography is a useful means of determining whether or not to ligate the patent ductus arteriosus (PDA) in premature infants with respiratory distress syndrome and PDA. The magnitude of the left-to-right ductal shunt can be gauged by measuring the diameter of the left atrium (LA) and aortic root (AO) echocardiographically and by determining the LA/AO ratio. An increase in this ratio during weaning from assisted ventilation is an indication for ligation of the PDA. We recently used this criterion in treating 2 infants with respiratory distress syndrome and PDA.", "contents": "Effects of assisted ventilation on echocardiographic findings in two infants with patent ductus arteriosus. Echocardiography is a useful means of determining whether or not to ligate the patent ductus arteriosus (PDA) in premature infants with respiratory distress syndrome and PDA. The magnitude of the left-to-right ductal shunt can be gauged by measuring the diameter of the left atrium (LA) and aortic root (AO) echocardiographically and by determining the LA/AO ratio. An increase in this ratio during weaning from assisted ventilation is an indication for ligation of the PDA. We recently used this criterion in treating 2 infants with respiratory distress syndrome and PDA.", "PMID": 957745}
{"id": "pubmed23n0032_2842", "title": "Fatal air embolism following gunshot wound of the lung.", "content": "Air embolism following penetrating lung trauma has been reported infrequently and its existence is questioned. A death resulting from air embolism following a high-velocity gunshot wound is presented. Appropriate treatment and preventive measures are discussed.", "contents": "Fatal air embolism following gunshot wound of the lung. Air embolism following penetrating lung trauma has been reported infrequently and its existence is questioned. A death resulting from air embolism following a high-velocity gunshot wound is presented. Appropriate treatment and preventive measures are discussed.", "PMID": 957746}
{"id": "pubmed23n0032_2843", "title": "Perfusion lung scan patterns in 100 patients with bronchogenic carcinoma.", "content": "The relationship between size of the perfusion defect as seen by perfusion lung scan (PLS) and size of the mass lesion as seen radiologically was correlated with the presence of regional adenopathy in 100 consecutive patients with bronchogenic carcinoma. All patients underwent scanning before open thoracotomy or mediastinotomy and had histologic documentation of the disease. Each perfusion lung scan was classified as follows: (1) perfusion defect larger than the mass lesion. (2) perfusion defect of the same size as the mass lesion, or (3) no focal defect seen. Among patients with a larger perfusion defect 84 per cent were found to have regional lymph node involvements, whereas among patients in whom a larger defect was not present only 23 per cent had such extensions of the disease.", "contents": "Perfusion lung scan patterns in 100 patients with bronchogenic carcinoma. The relationship between size of the perfusion defect as seen by perfusion lung scan (PLS) and size of the mass lesion as seen radiologically was correlated with the presence of regional adenopathy in 100 consecutive patients with bronchogenic carcinoma. All patients underwent scanning before open thoracotomy or mediastinotomy and had histologic documentation of the disease. Each perfusion lung scan was classified as follows: (1) perfusion defect larger than the mass lesion. (2) perfusion defect of the same size as the mass lesion, or (3) no focal defect seen. Among patients with a larger perfusion defect 84 per cent were found to have regional lymph node involvements, whereas among patients in whom a larger defect was not present only 23 per cent had such extensions of the disease.", "PMID": 957747}
{"id": "pubmed23n0032_2844", "title": "Pulmonary embolism: surgery in a hyperbaric chamber.", "content": "A 20-year-old man with a known history of thrombophlebitis experienced massive pulmonary embolism with hypoxia and shock. Embolectomy was performed with hyperbaric oxygenation for cardiopulmonary support. Ligation of the inferior vena cava was required postoperatively to prevent further embolism. Hyperbaric oxygenation is an effective method of cardiopulmonary support for pulmonary embolectomy.", "contents": "Pulmonary embolism: surgery in a hyperbaric chamber. A 20-year-old man with a known history of thrombophlebitis experienced massive pulmonary embolism with hypoxia and shock. Embolectomy was performed with hyperbaric oxygenation for cardiopulmonary support. Ligation of the inferior vena cava was required postoperatively to prevent further embolism. Hyperbaric oxygenation is an effective method of cardiopulmonary support for pulmonary embolectomy.", "PMID": 957748}
{"id": "pubmed23n0032_2845", "title": "Postpneumonectomy empyema in pulmonary carcinoma patients. Treatment with antibiotic irrigation and closed-chest drainage.", "content": "Seven patients with postpneumonectomy empyema who had had pulmonary carcinoma were treated with intrapleural antibiotic irrigation and closed-chest drainage. Two tubes were used to irrigate and drain the cavity. Although most patients had a bronchopleural or esophagopleural fistula, the treatment was successful in every case. Three of the 7 patients died of far-advanced carcinoma 1 to 2 years postoperatively, but none died of sequela of the empyema. In 3 patients with bronchopleural fistula, empyema recurred during the first postoperative year. However, it responded well to repeated irrigation and drainage. This simple, time-saving, and easily repeatable regimen proved to be both effective and also very comfortable for the patient. It has none of the disadvantages of open thoracic drainage or mutilating thoracoplasty.", "contents": "Postpneumonectomy empyema in pulmonary carcinoma patients. Treatment with antibiotic irrigation and closed-chest drainage. Seven patients with postpneumonectomy empyema who had had pulmonary carcinoma were treated with intrapleural antibiotic irrigation and closed-chest drainage. Two tubes were used to irrigate and drain the cavity. Although most patients had a bronchopleural or esophagopleural fistula, the treatment was successful in every case. Three of the 7 patients died of far-advanced carcinoma 1 to 2 years postoperatively, but none died of sequela of the empyema. In 3 patients with bronchopleural fistula, empyema recurred during the first postoperative year. However, it responded well to repeated irrigation and drainage. This simple, time-saving, and easily repeatable regimen proved to be both effective and also very comfortable for the patient. It has none of the disadvantages of open thoracic drainage or mutilating thoracoplasty.", "PMID": 957749}
{"id": "pubmed23n0032_2846", "title": "A safe method of thoracentesis.", "content": "A simple and safe method for therapeutic and diagnostic thoracentesis is described. The application of this technique of thoracentesis may decrease the chance of injury to the lung during the procedure. The complete evacuation of the pleural cavity may reduce the chance of development of adhesions. Repeated aspirations and instillation of medications with the same system left in the thoracic cavity may also be possible.", "contents": "A safe method of thoracentesis. A simple and safe method for therapeutic and diagnostic thoracentesis is described. The application of this technique of thoracentesis may decrease the chance of injury to the lung during the procedure. The complete evacuation of the pleural cavity may reduce the chance of development of adhesions. Repeated aspirations and instillation of medications with the same system left in the thoracic cavity may also be possible.", "PMID": 957750}
{"id": "pubmed23n0032_2847", "title": "Pulmonary artery conduits in infants younger than six months of age.", "content": "Ten infants under 6 months of age with right ventricular-pulmonary artery discontinuity have undergone reparative operations. Five infants had either Type I or Type II truncus and 5 had pulmonary atersia with some discontinuity between right and left pulmonary arteries. There have been 4 survivors in each group. The one death in each category was due to pulmonary hypertension. The remaining 8 patients have low pulmonary vascular resistance. The survival rate after the reparative technique in infancy appears better than that with other forms of palliative treatment, such as banding of shunts, in these severly ill patients.", "contents": "Pulmonary artery conduits in infants younger than six months of age. Ten infants under 6 months of age with right ventricular-pulmonary artery discontinuity have undergone reparative operations. Five infants had either Type I or Type II truncus and 5 had pulmonary atersia with some discontinuity between right and left pulmonary arteries. There have been 4 survivors in each group. The one death in each category was due to pulmonary hypertension. The remaining 8 patients have low pulmonary vascular resistance. The survival rate after the reparative technique in infancy appears better than that with other forms of palliative treatment, such as banding of shunts, in these severly ill patients.", "PMID": 957752}
{"id": "pubmed23n0032_2848", "title": "Arterial prosthesis of microporous expanded polytetrafluoroethylene for construction of aorta-pulmonary shunts.", "content": "A new arterial prosthesis made of polytetrafluoroethylene (OTFE) was evaluated in 10 infants with complex cyanotic congenital heart disease. All grafts used were 4 mm. in diameter and varied in length from 0.8 to 6 cm. The grafts were anastomosed to the main pulmonary artery or its bifurcation in 8 infants and to the right and left pulmonary arteries in one each. There were two early deaths, one of which was related to shunt failure. The advantages of a shunt to the main pulmonary artery are obvious, and the intraoperative procedure is facilitated with the prosthesis. Follow-up averages 9 months in the 8 survivors, and the patients have nearly doubled their preoperative weight. A shunt murmur is present in each case and the children have mild-to-moderate cyanosis at rest. Repeat aortograms in 2 patients, 8 and 10 months postoperatively, show a smooth graft without luminal narrowing. The aortic oxygen saturations were 73 per cent. The PTFE graft and/or anastomoses will not grow with the growth of the child and therefore may not accommodate growth by increased flow. This may prove to be a limiting factor in its long-term use in fants. We reserve the use of these grafts for infants with complex cyanotic defects undergoing emergency surgery or in older children in whom a conventional shunt is not possible or a previous shunt has failed.", "contents": "Arterial prosthesis of microporous expanded polytetrafluoroethylene for construction of aorta-pulmonary shunts. A new arterial prosthesis made of polytetrafluoroethylene (OTFE) was evaluated in 10 infants with complex cyanotic congenital heart disease. All grafts used were 4 mm. in diameter and varied in length from 0.8 to 6 cm. The grafts were anastomosed to the main pulmonary artery or its bifurcation in 8 infants and to the right and left pulmonary arteries in one each. There were two early deaths, one of which was related to shunt failure. The advantages of a shunt to the main pulmonary artery are obvious, and the intraoperative procedure is facilitated with the prosthesis. Follow-up averages 9 months in the 8 survivors, and the patients have nearly doubled their preoperative weight. A shunt murmur is present in each case and the children have mild-to-moderate cyanosis at rest. Repeat aortograms in 2 patients, 8 and 10 months postoperatively, show a smooth graft without luminal narrowing. The aortic oxygen saturations were 73 per cent. The PTFE graft and/or anastomoses will not grow with the growth of the child and therefore may not accommodate growth by increased flow. This may prove to be a limiting factor in its long-term use in fants. We reserve the use of these grafts for infants with complex cyanotic defects undergoing emergency surgery or in older children in whom a conventional shunt is not possible or a previous shunt has failed.", "PMID": 957753}
{"id": "pubmed23n0032_2849", "title": "Anatomic correction of transposition of the great vessels.", "content": "We present a new approach for anatomic correction of transposition of the great arteries. The two coronary arteries, with a piece of the aortic wall attached, are transposed to the posterior artery. The two aortic openings are closed with a patch. The aorta and pulmonary artery are transected, contraposed, ant then anastomosed. The interventricular septal defect is closed with a patch, through a right ventriculotomy approach, because the right ventricle is no longer part of the systemic circulation. Two patients, aged 3 months and 40 days weighing 4,200 and 3,700 grams, respectively, were operated upon with deep hypothermia and total circulatory arrest. There was good recovery from the operation, with normal cardiocirculatory conditions. Renal failure developed in the first patient, and she died on the third postoperative day. During this time the cardiocirculatory conditions were good. The second patient made an uneventful recovery. Hemodynamic studies 20 days after the operation showed complete correction of the malformation. Five and one-half months after the operation, he weighs 7,500 grams, and his development is very good. We believe that this operation will be reproducible by most cardiovascular septal defect and pulmonary hypertension.", "contents": "Anatomic correction of transposition of the great vessels. We present a new approach for anatomic correction of transposition of the great arteries. The two coronary arteries, with a piece of the aortic wall attached, are transposed to the posterior artery. The two aortic openings are closed with a patch. The aorta and pulmonary artery are transected, contraposed, ant then anastomosed. The interventricular septal defect is closed with a patch, through a right ventriculotomy approach, because the right ventricle is no longer part of the systemic circulation. Two patients, aged 3 months and 40 days weighing 4,200 and 3,700 grams, respectively, were operated upon with deep hypothermia and total circulatory arrest. There was good recovery from the operation, with normal cardiocirculatory conditions. Renal failure developed in the first patient, and she died on the third postoperative day. During this time the cardiocirculatory conditions were good. The second patient made an uneventful recovery. Hemodynamic studies 20 days after the operation showed complete correction of the malformation. Five and one-half months after the operation, he weighs 7,500 grams, and his development is very good. We believe that this operation will be reproducible by most cardiovascular septal defect and pulmonary hypertension.", "PMID": 957754}
{"id": "pubmed23n0032_2850", "title": "Transient protein-losing enteropathy secondary to elevated caval pressures and caval obstruction after the Mustard procedure.", "content": "Protein-losing enteropathy, documented by 51Cr-labeled albumin excretion studies, was demonstrated in a 24-month-old girl following the Mustard operation for complete transposition. Apparent postoperative inferior vena cava obstruction had been diagnosed by angiocardiograms and both caval pressure were found to be elevated. There was no evidence of primary renal or hepatic disease or cardiac failure. This problem may occur more frequently than has been recognized.", "contents": "Transient protein-losing enteropathy secondary to elevated caval pressures and caval obstruction after the Mustard procedure. Protein-losing enteropathy, documented by 51Cr-labeled albumin excretion studies, was demonstrated in a 24-month-old girl following the Mustard operation for complete transposition. Apparent postoperative inferior vena cava obstruction had been diagnosed by angiocardiograms and both caval pressure were found to be elevated. There was no evidence of primary renal or hepatic disease or cardiac failure. This problem may occur more frequently than has been recognized.", "PMID": 957755}
{"id": "pubmed23n0032_2851", "title": "Anatomic variations in underdeveloped right ventricle related to tricuspid atresia and stenosis.", "content": "In the anatomy of 416 hearts, the seat of tricuspid stenosis or atresia is examined, with special reference to Fontan-like surgical procedures. A classification is offered which includes cases with and without regular or inverted transposition, and with decreased or increased pulmonary flow. The size and thickness of the right atrium, the size and architecture of the right ventricle, the size of the pulmonary tree, the types of atrial and ventricular septal defects, the condition of the mitral valve, and the size and thickness of the left atrium and left ventricle are analyzed. In addition the various intracardiac and extracardiac abnormalities are enumerated. Reference is also made to the tendency of the aorta and pulmonary trunk to override the septum, in some cases producing double-outlet left ventricle. It is considered that many cases of tricuspid valve atresia and stenosis with or without transposition may be amenable to Fontan-like procedures in the proper age group. All the above anatomic considerations have a bearing on the suitability and type of operative tricuspid bypass procedures, and they may influence the prognosis of surgical therapy.", "contents": "Anatomic variations in underdeveloped right ventricle related to tricuspid atresia and stenosis. In the anatomy of 416 hearts, the seat of tricuspid stenosis or atresia is examined, with special reference to Fontan-like surgical procedures. A classification is offered which includes cases with and without regular or inverted transposition, and with decreased or increased pulmonary flow. The size and thickness of the right atrium, the size and architecture of the right ventricle, the size of the pulmonary tree, the types of atrial and ventricular septal defects, the condition of the mitral valve, and the size and thickness of the left atrium and left ventricle are analyzed. In addition the various intracardiac and extracardiac abnormalities are enumerated. Reference is also made to the tendency of the aorta and pulmonary trunk to override the septum, in some cases producing double-outlet left ventricle. It is considered that many cases of tricuspid valve atresia and stenosis with or without transposition may be amenable to Fontan-like procedures in the proper age group. All the above anatomic considerations have a bearing on the suitability and type of operative tricuspid bypass procedures, and they may influence the prognosis of surgical therapy.", "PMID": 957756}
{"id": "pubmed23n0032_2852", "title": "Leiomyosarcoma of the superior vena cava. A first case with resection.", "content": "Leiomysarcomas, rare malignant tumors of the veins, are located predominantly in the inferior vena cava. We report our experience with a leiomyosarcoma in the superior vena cava of a 44-year-old white man. The lesion had been locally excised and then recurred 2 1/2 years later. The recurrent tumor was widely excised and the superior vena cava was repaired with a saphenous vein patch graft. The area of the recurrence was treated with cobalt 60 therapy. Four years later, 6 1/2 years after initial treatment, the patient is alive and well without evidence of recurrence.", "contents": "Leiomyosarcoma of the superior vena cava. A first case with resection. Leiomysarcomas, rare malignant tumors of the veins, are located predominantly in the inferior vena cava. We report our experience with a leiomyosarcoma in the superior vena cava of a 44-year-old white man. The lesion had been locally excised and then recurred 2 1/2 years later. The recurrent tumor was widely excised and the superior vena cava was repaired with a saphenous vein patch graft. The area of the recurrence was treated with cobalt 60 therapy. Four years later, 6 1/2 years after initial treatment, the patient is alive and well without evidence of recurrence.", "PMID": 957757}
{"id": "pubmed23n0032_2853", "title": "The dynamic aortic root. Its role in aortic valve function.", "content": "By attaching appropriate measuring devices to the wall of an intact aortic root at the level of leaflet coaptation, we have measured a 16 per cent diameter change during each cardiac cycle. The dimensional changes observed can by themselves explain aortic valve function and obviate the postulation that the leaflets shorten and lengthen during each cardiac cycle. The tissue composition of the aortic root and leaflets is more compatible with this theory than with other postulations. Such a dynamic aortic root may explain the longevity of the actual aortic leaflets, in that leaflet fatigue stress is minimized by changes in aortic root dimension.", "contents": "The dynamic aortic root. Its role in aortic valve function. By attaching appropriate measuring devices to the wall of an intact aortic root at the level of leaflet coaptation, we have measured a 16 per cent diameter change during each cardiac cycle. The dimensional changes observed can by themselves explain aortic valve function and obviate the postulation that the leaflets shorten and lengthen during each cardiac cycle. The tissue composition of the aortic root and leaflets is more compatible with this theory than with other postulations. Such a dynamic aortic root may explain the longevity of the actual aortic leaflets, in that leaflet fatigue stress is minimized by changes in aortic root dimension.", "PMID": 957758}
{"id": "pubmed23n0032_2854", "title": "Thrombus of inferior vena cava presenting as right atrial tumor. Roentgenographic, phonoechocardiographic, angiographic, and surgical findings.", "content": "A large tumor of the right atrium was found to be only the precimal end of a bland thrombus of the inferior vena cava. Of special interest were certain echocardiographic findings, including persistent delay in closure of the tricuspid valve after successful removal of the thrombus.", "contents": "Thrombus of inferior vena cava presenting as right atrial tumor. Roentgenographic, phonoechocardiographic, angiographic, and surgical findings. A large tumor of the right atrium was found to be only the precimal end of a bland thrombus of the inferior vena cava. Of special interest were certain echocardiographic findings, including persistent delay in closure of the tricuspid valve after successful removal of the thrombus.", "PMID": 957759}
{"id": "pubmed23n0032_2855", "title": "Pulmonary valve cyst in an adult. Case report.", "content": "A case is described of an asymptomatic woman who had typical findings of mild pulmonic stenosis. Right heart catheterization revealed a 44 mm. Hg gradient across the pulmonary outflow tract, and a filling defect was discovered by angiography. On surgical exploration, the defect was found to be a benign cyst attached to the anterior leaflet of the pulmonary valve.", "contents": "Pulmonary valve cyst in an adult. Case report. A case is described of an asymptomatic woman who had typical findings of mild pulmonic stenosis. Right heart catheterization revealed a 44 mm. Hg gradient across the pulmonary outflow tract, and a filling defect was discovered by angiography. On surgical exploration, the defect was found to be a benign cyst attached to the anterior leaflet of the pulmonary valve.", "PMID": 957760}
{"id": "pubmed23n0032_2856", "title": "Rapid overdrive pacing of refractory tachyarrhythmias in patients after open-heart surgery.", "content": "The efficacy of rapid ventricular pacemaker overdrive in the treatment of supraventricular and ventricular tachyarrhythmias is presented as a new approach to the management of these rhythm disorders inpatients after cardiac surgery. This mode of therapy is exemplified in the control of heart rate and return of normal sinus rhythm in patients with both types of tachyarrhythmias refractory to conventional antiarrhythmic agents. In addition, the pathogenesis and mechanisms of pacemaker overdrive in termination these rhythm disturbances are delineated.", "contents": "Rapid overdrive pacing of refractory tachyarrhythmias in patients after open-heart surgery. The efficacy of rapid ventricular pacemaker overdrive in the treatment of supraventricular and ventricular tachyarrhythmias is presented as a new approach to the management of these rhythm disorders inpatients after cardiac surgery. This mode of therapy is exemplified in the control of heart rate and return of normal sinus rhythm in patients with both types of tachyarrhythmias refractory to conventional antiarrhythmic agents. In addition, the pathogenesis and mechanisms of pacemaker overdrive in termination these rhythm disturbances are delineated.", "PMID": 957761}
{"id": "pubmed23n0032_2857", "title": "Quinidine syncope. A report of successful treatment with bretylium tosylate.", "content": "Quinidine syncope is an unpredictable side effect of quinidine administrateion which has a mortality rate of 11.5 per cent when managed with conventional drugs and direct-current countershock. In 2 patients with quindine syncope, the arrhythmias were effectively controlled within 30 minutes following the slow infusion of 150 mg. of bretylium tosylate. This control is contrasted with 2 cases managed in the conventional manner. Bretylium tosylate is a quaternary amine with unique antifibrillatory properties and appears to have a significant role in the management of these arrhylmias.", "contents": "Quinidine syncope. A report of successful treatment with bretylium tosylate. Quinidine syncope is an unpredictable side effect of quinidine administrateion which has a mortality rate of 11.5 per cent when managed with conventional drugs and direct-current countershock. In 2 patients with quindine syncope, the arrhythmias were effectively controlled within 30 minutes following the slow infusion of 150 mg. of bretylium tosylate. This control is contrasted with 2 cases managed in the conventional manner. Bretylium tosylate is a quaternary amine with unique antifibrillatory properties and appears to have a significant role in the management of these arrhylmias.", "PMID": 957762}
{"id": "pubmed23n0032_2858", "title": "The role of lymphatics in the formation of ascites complicating schistosomal hepatic fibrosis.", "content": "The total protein content in plasma, ascitic fluid, thoracic duct lymph, hepatic and intestinal lymph was studied in a series of 15 patients suffering from schistosomal hepatic fibrosis and intractable ascites. Pure schistosomal cases with presinusoidal resistance to portal blood flow have excessive thoracic duct lymph low in protein. The main source of such excess lymph is the extra-hepatic portal bed. Ascitic fluid in such patients has a low protein content and has the character of a transudate. The bulk of such peritoneal fluid seems to originate largely from the excess extrahepatic portal lymph.", "contents": "The role of lymphatics in the formation of ascites complicating schistosomal hepatic fibrosis. The total protein content in plasma, ascitic fluid, thoracic duct lymph, hepatic and intestinal lymph was studied in a series of 15 patients suffering from schistosomal hepatic fibrosis and intractable ascites. Pure schistosomal cases with presinusoidal resistance to portal blood flow have excessive thoracic duct lymph low in protein. The main source of such excess lymph is the extra-hepatic portal bed. Ascitic fluid in such patients has a low protein content and has the character of a transudate. The bulk of such peritoneal fluid seems to originate largely from the excess extrahepatic portal lymph.", "PMID": 957764}
{"id": "pubmed23n0032_2859", "title": "Percutaneous trans hepatic lymphography: evaluation in schistosomal hepatic fibrosis.", "content": "The increased interest in lymph and lymphatics has cast its mantle over the portal circulation. Lymphography has contributed greately to our knowledge. In the present study percutaneous transhepatic lymphography showed some of the factors sharing in the production of portal hypertension in schistosomal hepatic fibrosis, and gave a further evidence that the liver is not a source of excess lymph production in hepatic lesions associated with presinusoidal block to portal blood flow. Hepatic lymphatics were opacified in cases with mixed cirrhosis and schistosomal hepatic fibrosis as the sinusoidal pressure is elevated with subsequent cases increase in hepatic lymph production.", "contents": "Percutaneous trans hepatic lymphography: evaluation in schistosomal hepatic fibrosis. The increased interest in lymph and lymphatics has cast its mantle over the portal circulation. Lymphography has contributed greately to our knowledge. In the present study percutaneous transhepatic lymphography showed some of the factors sharing in the production of portal hypertension in schistosomal hepatic fibrosis, and gave a further evidence that the liver is not a source of excess lymph production in hepatic lesions associated with presinusoidal block to portal blood flow. Hepatic lymphatics were opacified in cases with mixed cirrhosis and schistosomal hepatic fibrosis as the sinusoidal pressure is elevated with subsequent cases increase in hepatic lymph production.", "PMID": 957765}
{"id": "pubmed23n0032_2860", "title": "Cervical lymphato-venous shunt in treatment of ascites in caval-constricted dogs and in patients with hepatic cirrhosis. Experimental observations and 7 years clinical experience.", "content": "In experimental ascites produced by inferior vena caval constriction there is a pressure gradient at the thoracic duct-venous junction suggesting a functional obstacle to lymph flow. Abdominal fluid and protein are transported mainly by the right lymph trunk and not by the thoracic duct. External drainage of the thoracic duct or construction of a new, wider thoracic duct-venous anastomosis facilitates, however, transport of excess capillary filtrate formed in the liver and decreases fluid spillage into the peritoneal cavity. Construction of a cervical lymphato-venous anastomosis was attempted in 21 patients, 20 of whom had cirrhosis, and the operation was technically successful in 16 cases. Operation was helpful in cirrhotic patients with rapid ascites formation, who required less frequent abdominal paracenteses operation. The shunt was without benefit in prevention of oesophageal varix bleedings, and it should not be attempted in patients with icterus and severe deterioration of liver function.", "contents": "Cervical lymphato-venous shunt in treatment of ascites in caval-constricted dogs and in patients with hepatic cirrhosis. Experimental observations and 7 years clinical experience. In experimental ascites produced by inferior vena caval constriction there is a pressure gradient at the thoracic duct-venous junction suggesting a functional obstacle to lymph flow. Abdominal fluid and protein are transported mainly by the right lymph trunk and not by the thoracic duct. External drainage of the thoracic duct or construction of a new, wider thoracic duct-venous anastomosis facilitates, however, transport of excess capillary filtrate formed in the liver and decreases fluid spillage into the peritoneal cavity. Construction of a cervical lymphato-venous anastomosis was attempted in 21 patients, 20 of whom had cirrhosis, and the operation was technically successful in 16 cases. Operation was helpful in cirrhotic patients with rapid ascites formation, who required less frequent abdominal paracenteses operation. The shunt was without benefit in prevention of oesophageal varix bleedings, and it should not be attempted in patients with icterus and severe deterioration of liver function.", "PMID": 957766}
{"id": "pubmed23n0032_2861", "title": "Lymphographical findings in disseminated tuberculosis without radiological lung manifestations.", "content": "Lymphographical findings are presented in three patients with verified tuberculosis. The diagnosis of tuberculosis was difficult in these patients because there were no actual changes in chest radiographs and the clinical findings were generally nonspecific. In each patient, lymphographic changes in lumbar lymph nodes were established. These changes corresponded to those described in the literature in connection with tuberculosis. Thus, the lymphographical findings can here be considered to be a further criterion for the diagnosis of tuberculosis. Lymphographical follow-up also proved beneficial in the follow-up of treatment results in one patient. Suspected tuberculosis, in which local findings cannot be made available by other means, can be regarded as one of the indications for a lymphography.", "contents": "Lymphographical findings in disseminated tuberculosis without radiological lung manifestations. Lymphographical findings are presented in three patients with verified tuberculosis. The diagnosis of tuberculosis was difficult in these patients because there were no actual changes in chest radiographs and the clinical findings were generally nonspecific. In each patient, lymphographic changes in lumbar lymph nodes were established. These changes corresponded to those described in the literature in connection with tuberculosis. Thus, the lymphographical findings can here be considered to be a further criterion for the diagnosis of tuberculosis. Lymphographical follow-up also proved beneficial in the follow-up of treatment results in one patient. Suspected tuberculosis, in which local findings cannot be made available by other means, can be regarded as one of the indications for a lymphography.", "PMID": 957767}
{"id": "pubmed23n0032_2862", "title": "Lymphatics of the canine papillary muscles.", "content": "A relatively dense concentration of lymphatics in the region of the papillary muscles of the canine left ventricle has been described. It is suggested that these channels may play a role in the development of pathologic changes affecting the mitral valve apparatus which could subsequently cause valve dysfunction.", "contents": "Lymphatics of the canine papillary muscles. A relatively dense concentration of lymphatics in the region of the papillary muscles of the canine left ventricle has been described. It is suggested that these channels may play a role in the development of pathologic changes affecting the mitral valve apparatus which could subsequently cause valve dysfunction.", "PMID": 957768}
{"id": "pubmed23n0032_2863", "title": "The bicuspid nature of the valves of the peripheral collecting lymphatic vessels of the dog.", "content": "A controversy presently exists about the structure of lymphatic valves, the bicuspid structure vs. the funnel-like architechture. This present study, along with a review of the literature was undertaken in an attempt to clarify this controversy. Twenty lymphatic valves from the collecting lymphatic channels draining the back paw region in four dogs were examined and several were photographed. The bicuspid nature and the three dimensional anatomy of these valves is clearly demonstrated.", "contents": "The bicuspid nature of the valves of the peripheral collecting lymphatic vessels of the dog. A controversy presently exists about the structure of lymphatic valves, the bicuspid structure vs. the funnel-like architechture. This present study, along with a review of the literature was undertaken in an attempt to clarify this controversy. Twenty lymphatic valves from the collecting lymphatic channels draining the back paw region in four dogs were examined and several were photographed. The bicuspid nature and the three dimensional anatomy of these valves is clearly demonstrated.", "PMID": 957769}
{"id": "pubmed23n0032_2864", "title": "The early postprandial dumping syndrome: clinical manifestations and pathogenesis.", "content": "Our present concept of the pathogenesis of the early postprandial dumping syndrome is well summarized by Jesseph. Resection, division or bypass of the sphincter mechanism at the gastric outlet permits rapid passage of hyperosmolar material into the upper small intestine. This provides direct stimulation of the enterochromaffin (argentaffin) cells in the mucosa, which are highly concentrated here. The hyperosmolarity pulls fluid into the intestine resulting in a fall in plasma volume and distention of the intestine, further stimulating secretion by the argentaffin tissue. The plasma volume per se probably has little, if anything, to do with the symptoms produced although the outpouring of intravascular fluid into the intestinal lumen probably contributes to intestinal hyperperistalsis and the resultant symptoms of intestinal hurry. Although other sources are possible, studies to date would indicate that the argentaffin cells are the major source of humoral agents. In addition to serotonin, at least one vasoactive polypeptide, bradykinin, has been identified. It is likely that others are present and pharmacologic therapy will probably not be successful until these are more completely identified and characterized. The known biologic effects of serotonin and the kinins can certainly account for all the vasomotor and gastrointestinal symptoms characterizing the early postprandial dumping syndrome.", "contents": "The early postprandial dumping syndrome: clinical manifestations and pathogenesis. Our present concept of the pathogenesis of the early postprandial dumping syndrome is well summarized by Jesseph. Resection, division or bypass of the sphincter mechanism at the gastric outlet permits rapid passage of hyperosmolar material into the upper small intestine. This provides direct stimulation of the enterochromaffin (argentaffin) cells in the mucosa, which are highly concentrated here. The hyperosmolarity pulls fluid into the intestine resulting in a fall in plasma volume and distention of the intestine, further stimulating secretion by the argentaffin tissue. The plasma volume per se probably has little, if anything, to do with the symptoms produced although the outpouring of intravascular fluid into the intestinal lumen probably contributes to intestinal hyperperistalsis and the resultant symptoms of intestinal hurry. Although other sources are possible, studies to date would indicate that the argentaffin cells are the major source of humoral agents. In addition to serotonin, at least one vasoactive polypeptide, bradykinin, has been identified. It is likely that others are present and pharmacologic therapy will probably not be successful until these are more completely identified and characterized. The known biologic effects of serotonin and the kinins can certainly account for all the vasomotor and gastrointestinal symptoms characterizing the early postprandial dumping syndrome.", "PMID": 957773}
{"id": "pubmed23n0032_2865", "title": "Gastric remnant carcinoma.", "content": "Over 1200 cases of carcinoma of the gastric remnant have been reported in the literature. There is an increase of this type of carcinoma in postoperative stomachs with atrophic gastritis and intestinal metaplasia. The cause and effect relationships remain to be fully elucidated. In patients with late postgastrectomy symptoms, carcinoma of the gastric remnant should be considered in the differential diagnosis. In a study of 350 asymptomatic patients who were more than 20 years from Billroth II gastric resection, 14 carcinomas were discovered in the region of the stoma. Preoperatively, gross endoscopic appearance and multiple biopsies will usually provide the diagnosis. At the time of revisional surgery, frozen section of gastric biopsies or the resected specimen may be necessary to exclude the diagnosis. At present there is widespread interest in several procedures in the treatment of benign ulcer disease. In selected patients, proximal gastric vagotomy is receiving particular interest. It remains to be determined what, if any, gastric mucosal alterations occur. Since the pyloric mechanism is intact, no stoma is created and no portion of the stomach resected; long-term followup of these patients will be of interest. Information as to the cause of gastric remnant carcinoma can be forthcoming only by evaluation of all groups of patients requiring gastric surgery for benign disease. At the same time, further investigation of patients with gastric carcinoma without prior resection who have atrophic gastritis and intestinal metaplasia is also necessary. The histologic type of carcinoma that develops in the gastric remnant is usually more favorable for surgical cure than those seen in the intact stomach. This means that early diagnosis by radiologic and endoscopic study of postgastrectomy patients developing symptoms is highly desirable. Because of the long interval between gastrectomy and gastric remnant carcinoma these patients are often in the older age group. The location of the lesion in the remaining proximal stomach will nearly always require total gastrectomy. This plus the age factor means that the operative mortality will be rather high. We are unable to explain why in 22 years of observing postgastrectomy patients we have seen only one case of gastric remnant carcinoma. This patient was successfully treated by left transpleural transdiaphragmatic total gastrectomy with Roux-en-Y esophagojejunostomy. This method is particulary easy in the patient who has has an antecolic Billroth II gastrectomy. If the jejunum cannot be adequately mobilized through a radial incision extending laterally from the esophageal hiatus, we use a peripheral diaphragmatic incision in circumferential fashion. This gives excellent exposure of the upper abdominal contents and also preserves the phrenic nerve. As a result, ventilatory function of the left leaf of the diaphragm is preserved postoperatively.", "contents": "Gastric remnant carcinoma. Over 1200 cases of carcinoma of the gastric remnant have been reported in the literature. There is an increase of this type of carcinoma in postoperative stomachs with atrophic gastritis and intestinal metaplasia. The cause and effect relationships remain to be fully elucidated. In patients with late postgastrectomy symptoms, carcinoma of the gastric remnant should be considered in the differential diagnosis. In a study of 350 asymptomatic patients who were more than 20 years from Billroth II gastric resection, 14 carcinomas were discovered in the region of the stoma. Preoperatively, gross endoscopic appearance and multiple biopsies will usually provide the diagnosis. At the time of revisional surgery, frozen section of gastric biopsies or the resected specimen may be necessary to exclude the diagnosis. At present there is widespread interest in several procedures in the treatment of benign ulcer disease. In selected patients, proximal gastric vagotomy is receiving particular interest. It remains to be determined what, if any, gastric mucosal alterations occur. Since the pyloric mechanism is intact, no stoma is created and no portion of the stomach resected; long-term followup of these patients will be of interest. Information as to the cause of gastric remnant carcinoma can be forthcoming only by evaluation of all groups of patients requiring gastric surgery for benign disease. At the same time, further investigation of patients with gastric carcinoma without prior resection who have atrophic gastritis and intestinal metaplasia is also necessary. The histologic type of carcinoma that develops in the gastric remnant is usually more favorable for surgical cure than those seen in the intact stomach. This means that early diagnosis by radiologic and endoscopic study of postgastrectomy patients developing symptoms is highly desirable. Because of the long interval between gastrectomy and gastric remnant carcinoma these patients are often in the older age group. The location of the lesion in the remaining proximal stomach will nearly always require total gastrectomy. This plus the age factor means that the operative mortality will be rather high. We are unable to explain why in 22 years of observing postgastrectomy patients we have seen only one case of gastric remnant carcinoma. This patient was successfully treated by left transpleural transdiaphragmatic total gastrectomy with Roux-en-Y esophagojejunostomy. This method is particulary easy in the patient who has has an antecolic Billroth II gastrectomy. If the jejunum cannot be adequately mobilized through a radial incision extending laterally from the esophageal hiatus, we use a peripheral diaphragmatic incision in circumferential fashion. This gives excellent exposure of the upper abdominal contents and also preserves the phrenic nerve. As a result, ventilatory function of the left leaf of the diaphragm is preserved postoperatively.", "PMID": 957774}
{"id": "pubmed23n0032_2866", "title": "Conformational transitions of polypeptides in ternary solvent systems.", "content": "Phase diagrams for conformational transitions of polypeptides in ternary organic solvent systems containing two active and one inert component are calculated for a model which takes into account possible interaction of the two active components with each other as well as with the polypeptide backbone. The results of experimental determinations of conformational transitions and isothermal phase boundaries for poly(beta-benzyl L-asparate) (PBA) in three-model solvent systems are in generally good accord with the theory. The solvent systems are: (a) 1-chloropropionic acid-dichloroacetic acid (DCA)-1,1,2,2-tetrachloroethane (TCE), (b) monochloroacetic acid-DCA-TCE, and (c) dimethyl sulfoxide-DCA-TCE.", "contents": "Conformational transitions of polypeptides in ternary solvent systems. Phase diagrams for conformational transitions of polypeptides in ternary organic solvent systems containing two active and one inert component are calculated for a model which takes into account possible interaction of the two active components with each other as well as with the polypeptide backbone. The results of experimental determinations of conformational transitions and isothermal phase boundaries for poly(beta-benzyl L-asparate) (PBA) in three-model solvent systems are in generally good accord with the theory. The solvent systems are: (a) 1-chloropropionic acid-dichloroacetic acid (DCA)-1,1,2,2-tetrachloroethane (TCE), (b) monochloroacetic acid-DCA-TCE, and (c) dimethyl sulfoxide-DCA-TCE.", "PMID": 957770}
{"id": "pubmed23n0032_2867", "title": "Hematologic abnormalities following gastric resection.", "content": "The anemia observed in patients with partial gastric resection results from a complex interrelationship of deficiencies of these three important hematemics-iron, vitamin B12, and folic acid. Reliance upon morphological evidence of anemia in the peripheral blood smear may be difficult and confusing since deficiency of one hematemic may mask the coexisting deficiency of another. It is common for deficiencies of more than one hematemic to occur in these patients. A number of studies have demonstrated the masking effect of iron deficiency on concurrent vitamin B12 or folic acid deficiency. In addition, the morphologic hallmarks of iron deficiency may be modified by the presence of deficiencies of either vitamin B12 or folate or both. Full hematologic recovery may not occur until more than one hematemic is given to the patient. It is our policy at the University of Florida to rely on serum levels of these three hematemics, especially vitamin B12 and iron, to detect the cause of the anemia in a patient with partial gastric resection. Less reliance is placed upon the appearance of the peripheral smear because of the masking effect described above. If either the serum iron level or vitamin B12 level is decreased, we treat the patient with a preparation such as ferrous sulfate (300 mg. orally three times a day) and vitamin B12 (100 mug. intramuscularly once a month). We are less concerned with folic acid deficiency because of its relatively infrequent occurrence in this setting and because a good diet will usually suffice as adequate therapy for the folic acid deficiency when present. In patients who have had partial gastric resection but who are not anemic, we assess vitamin B12 absorption by the conventional vitamin B12 urinary excretion test (Schilling test) on a yearly basis since deficiency of this hematemic may lead to serious hematologic and neurologic sequelae. If the patient manifests decreased vitamin B12 absorption uncorrected by the administration of pancreatic extract or antibiotics, this patient is also treated with 100 mug. of vitamin B12 intramuscularly on a monthly basis. We have not evaluated the absorption of food B12 as suggested by Doscherholmen. Perhaps more attention should be paid to this aspect of vitamin B12 absorption in these patients. Indeed, because of the serious complications of vitamin B12 deficiency and the observations that deficiencies of this vitamin may occur even when the absorption of crystalline vitamin B12 is normal in the fasting state (the conventional Schilling test), some authors, such as Rygvold, have suggested that prophylactic vitamin B12 be administered to all patients with partial gastric resection.", "contents": "Hematologic abnormalities following gastric resection. The anemia observed in patients with partial gastric resection results from a complex interrelationship of deficiencies of these three important hematemics-iron, vitamin B12, and folic acid. Reliance upon morphological evidence of anemia in the peripheral blood smear may be difficult and confusing since deficiency of one hematemic may mask the coexisting deficiency of another. It is common for deficiencies of more than one hematemic to occur in these patients. A number of studies have demonstrated the masking effect of iron deficiency on concurrent vitamin B12 or folic acid deficiency. In addition, the morphologic hallmarks of iron deficiency may be modified by the presence of deficiencies of either vitamin B12 or folate or both. Full hematologic recovery may not occur until more than one hematemic is given to the patient. It is our policy at the University of Florida to rely on serum levels of these three hematemics, especially vitamin B12 and iron, to detect the cause of the anemia in a patient with partial gastric resection. Less reliance is placed upon the appearance of the peripheral smear because of the masking effect described above. If either the serum iron level or vitamin B12 level is decreased, we treat the patient with a preparation such as ferrous sulfate (300 mg. orally three times a day) and vitamin B12 (100 mug. intramuscularly once a month). We are less concerned with folic acid deficiency because of its relatively infrequent occurrence in this setting and because a good diet will usually suffice as adequate therapy for the folic acid deficiency when present. In patients who have had partial gastric resection but who are not anemic, we assess vitamin B12 absorption by the conventional vitamin B12 urinary excretion test (Schilling test) on a yearly basis since deficiency of this hematemic may lead to serious hematologic and neurologic sequelae. If the patient manifests decreased vitamin B12 absorption uncorrected by the administration of pancreatic extract or antibiotics, this patient is also treated with 100 mug. of vitamin B12 intramuscularly on a monthly basis. We have not evaluated the absorption of food B12 as suggested by Doscherholmen. Perhaps more attention should be paid to this aspect of vitamin B12 absorption in these patients. Indeed, because of the serious complications of vitamin B12 deficiency and the observations that deficiencies of this vitamin may occur even when the absorption of crystalline vitamin B12 is normal in the fasting state (the conventional Schilling test), some authors, such as Rygvold, have suggested that prophylactic vitamin B12 be administered to all patients with partial gastric resection.", "PMID": 957776}
{"id": "pubmed23n0032_2868", "title": "Malabsorption following gastric resection.", "content": "We have tried to stress the complexity of the bacterial ecology that may exist in the intestine of patients and experimental animals with small intestinal bacterial overgrowth. The multiplicity of organisms often makes the management of these patients quite frustrating. A number of metabolic derangements of varying severity may occur in any given patient. Although many of the observed abnormalities are secondary to disturbed events with the luminal environment of the small intestine, the significance of direct damage to the small intestinal epithelium has been emphasized. Since intestinal cultures are both cumbersome and difficult to perform on a routine basis, the use of labeled substrate breath tests will allow guided, outpatient therapy more easily than in the past. Since full correction of the malabsorption is seldom achieved with antibiotic therapy, nutritional supplementation deserves more attention than it has previously received.", "contents": "Malabsorption following gastric resection. We have tried to stress the complexity of the bacterial ecology that may exist in the intestine of patients and experimental animals with small intestinal bacterial overgrowth. The multiplicity of organisms often makes the management of these patients quite frustrating. A number of metabolic derangements of varying severity may occur in any given patient. Although many of the observed abnormalities are secondary to disturbed events with the luminal environment of the small intestine, the significance of direct damage to the small intestinal epithelium has been emphasized. Since intestinal cultures are both cumbersome and difficult to perform on a routine basis, the use of labeled substrate breath tests will allow guided, outpatient therapy more easily than in the past. Since full correction of the malabsorption is seldom achieved with antibiotic therapy, nutritional supplementation deserves more attention than it has previously received.", "PMID": 957777}
{"id": "pubmed23n0032_2869", "title": "The early postprandial dumping syndrome: prevention and treatment.", "content": "The early postprandial dumping syndrome can be prevented or minimized by the appropriate selection of the operative procedure to fit the patient and the peptic ulcer problem he presents, and by proper attention to diet in the early postoperative period. When it does occur, the syndrome usually responds favorably to dietary management and tends to spontaneously regress in severity with time. For these reasons further surgery is seldom required for the early postprandial dumping syndrome. In the patient who fails to improve with diet therapy and time and has disabling symptoms often accompanied by progressive malnutrition, revisional surgery should be undertaken. It is the objective of the surgeon to alter the reconstruction in such a way that emptying from the stomach or gastric remnant is delayed. Therefore, the upper small intestine dose not receive a large, rapidly introduced hyperosmolar bolus to initiate the release of humoral substances causing the syndrome. All revisions utilized are potentially ulcerogenic and if vagotomy has not been a part of the original procedure, it should routinely be performed at the time of revision. Patients who have primarily has a Billroth II gastrectomy will frequently improve markedly with simple conversion to a Billroth I reconstruction. This is particularly true when the residual stomach is moderately large (i.e., after antrectomy) and when the gastrojejunal stoma is larger in diameter than the normal jejunum. Under such circumstances approximately 80 per cent of patients will improve sufficiently so that a more complex procedure need not be utilized at once. Under all other conditions we prefer a 10 cm. segment of reversed jejunum anastomosed proximally to the gastric stump and distally to a 40 cm. isoperistaltic Roux-en-Y jejunal limb. This procedure is so successful that one can justify its use as first recourse even in the anatomically favorable Billroth II patient. It should be pointed out emphatically that an isoperistaltic jejunal interposition (Henley loop) has little or no effect on the early postprandial dumping syndrome and should not be considered. Plicated loops of intestine to recreate a gastric reservoir frequently fail to empty satisfactorily and the incidence of satisfactory results is too low to consider their utilization in surgical treatment of the dumping syndrome.", "contents": "The early postprandial dumping syndrome: prevention and treatment. The early postprandial dumping syndrome can be prevented or minimized by the appropriate selection of the operative procedure to fit the patient and the peptic ulcer problem he presents, and by proper attention to diet in the early postoperative period. When it does occur, the syndrome usually responds favorably to dietary management and tends to spontaneously regress in severity with time. For these reasons further surgery is seldom required for the early postprandial dumping syndrome. In the patient who fails to improve with diet therapy and time and has disabling symptoms often accompanied by progressive malnutrition, revisional surgery should be undertaken. It is the objective of the surgeon to alter the reconstruction in such a way that emptying from the stomach or gastric remnant is delayed. Therefore, the upper small intestine dose not receive a large, rapidly introduced hyperosmolar bolus to initiate the release of humoral substances causing the syndrome. All revisions utilized are potentially ulcerogenic and if vagotomy has not been a part of the original procedure, it should routinely be performed at the time of revision. Patients who have primarily has a Billroth II gastrectomy will frequently improve markedly with simple conversion to a Billroth I reconstruction. This is particularly true when the residual stomach is moderately large (i.e., after antrectomy) and when the gastrojejunal stoma is larger in diameter than the normal jejunum. Under such circumstances approximately 80 per cent of patients will improve sufficiently so that a more complex procedure need not be utilized at once. Under all other conditions we prefer a 10 cm. segment of reversed jejunum anastomosed proximally to the gastric stump and distally to a 40 cm. isoperistaltic Roux-en-Y jejunal limb. This procedure is so successful that one can justify its use as first recourse even in the anatomically favorable Billroth II patient. It should be pointed out emphatically that an isoperistaltic jejunal interposition (Henley loop) has little or no effect on the early postprandial dumping syndrome and should not be considered. Plicated loops of intestine to recreate a gastric reservoir frequently fail to empty satisfactorily and the incidence of satisfactory results is too low to consider their utilization in surgical treatment of the dumping syndrome.", "PMID": 957778}
{"id": "pubmed23n0032_2870", "title": "Complications following gastric surgery in children.", "content": "Although the diagnosis of peptic ulcer disease may be difficult in the pediatric age group, this problem is being faced increasingly by the physicians charged with the care of these children. Chronicity of symptoms has been documented in over 50 per cent of the patients demonstrating early peptic ulcers and therefore an aggressive approach to management has been advocated. Although the numbers are small, postgastrectomy syndromes of mechanical and nutritional nature appear less common in the young patient than in adults. Satisfactory growth and development are reported in nearly all series of patients followed after various gastric procedures. Both pyloroplasty and vagotomy and vagotomy and antrectomy appear to be extraordinarily well tolerated by the pediatric patient. Experimental evidence suggests that these patients should enjoy normal growth and development.", "contents": "Complications following gastric surgery in children. Although the diagnosis of peptic ulcer disease may be difficult in the pediatric age group, this problem is being faced increasingly by the physicians charged with the care of these children. Chronicity of symptoms has been documented in over 50 per cent of the patients demonstrating early peptic ulcers and therefore an aggressive approach to management has been advocated. Although the numbers are small, postgastrectomy syndromes of mechanical and nutritional nature appear less common in the young patient than in adults. Satisfactory growth and development are reported in nearly all series of patients followed after various gastric procedures. Both pyloroplasty and vagotomy and vagotomy and antrectomy appear to be extraordinarily well tolerated by the pediatric patient. Experimental evidence suggests that these patients should enjoy normal growth and development.", "PMID": 957779}
{"id": "pubmed23n0032_2871", "title": "Monte Carlo calculations on polypeptide chains. IX. A study of the effect of long-range interactions on the helix-coil transition.", "content": "A Monte Carlo statistical mechanical study of the helix-coil transition for a hard-sphere model of poly(L-alanine) has been conducted base on the theory of Lifson and Roig but including the effects of long-range interactions. A stochastic model of the kinetics of the helix-coil transition is presented, and a Monte Carlo stimulation of the kinetics based on this model was used to generate equilibrium chain samples, each chain of which consisted of Lifson-Roig weighted sequences of helix and coil residues. Each of the chains in this sample was then used many times by assigning at random specific sterically allowed coil states from a hard-sphere Ramachandran dipeptide map. Unperturbed properties were then calculated using this sample and perturbed properties by using only the non-self-conflicting subset. The properties calculated were the average degree of hydrogen bonding, the average length of a helical sequence, the mean-square end-to-end distance, the mean-square radius of gyration, and the distribution functions for the end-to-end distance and radius of gyration. This study was conducted at chain lengths 10, 34, and 85 residues. Helix-coil transition theory was fit to the perturbed transition curves in an attempt to ascertain if theory could then predict the perturbed values of the dimensions. For the hard-sphere model used in these calculations, it was found that current helix-coil transition theory does not predict the correct perturbed dimensions.", "contents": "Monte Carlo calculations on polypeptide chains. IX. A study of the effect of long-range interactions on the helix-coil transition. A Monte Carlo statistical mechanical study of the helix-coil transition for a hard-sphere model of poly(L-alanine) has been conducted base on the theory of Lifson and Roig but including the effects of long-range interactions. A stochastic model of the kinetics of the helix-coil transition is presented, and a Monte Carlo stimulation of the kinetics based on this model was used to generate equilibrium chain samples, each chain of which consisted of Lifson-Roig weighted sequences of helix and coil residues. Each of the chains in this sample was then used many times by assigning at random specific sterically allowed coil states from a hard-sphere Ramachandran dipeptide map. Unperturbed properties were then calculated using this sample and perturbed properties by using only the non-self-conflicting subset. The properties calculated were the average degree of hydrogen bonding, the average length of a helical sequence, the mean-square end-to-end distance, the mean-square radius of gyration, and the distribution functions for the end-to-end distance and radius of gyration. This study was conducted at chain lengths 10, 34, and 85 residues. Helix-coil transition theory was fit to the perturbed transition curves in an attempt to ascertain if theory could then predict the perturbed values of the dimensions. For the hard-sphere model used in these calculations, it was found that current helix-coil transition theory does not predict the correct perturbed dimensions.", "PMID": 957771}
{"id": "pubmed23n0032_2872", "title": "The afferent loop syndrome.", "content": "The afferent loop syndromes result from obstruction to the afferent jejunal loop. Acute ALS results from complete obstruction, usually occurs early after surgery and runs a devastatingly lethal course unless promptly treated by reoperation. In chronic ALS the obstruction is intermittent and produces a clinical syndrome from which a diagnostic histroy can usually be obtained. Although the exact incidence is unknown, it is certainly not rare, especially in antecolic Billroth II gastrectomies. Treatment consists of doing away with the afferent loop. In gastroenterostomy alone takedown of the anastomosis with a Weinberg pyloroplasty is the treatment of choice. The safest and simplest treatment for patients whose original operation was Billroth II gastrectomy is conversion to a Roux-en-Y procedure. In all cases vagotomy should be added unless previously performed. No medical treatment is available and patients with no other contraindication should have revisional surgery if symptoms are clinically significant. Both acute and chronic afferent loop syndromes should be completely prevented by appropriate choice of the initial operative procedure. The vagotomized stomach should be drained by pyloroplasty, not gastrojejunostomy. Vagotomy and antrectomy should be reconstructed with a Billroth I gastroduodenostomy. The Braun enteroanastomosis should be utilized after subtotal gastrectomy for carcinoma. The wider application of parietal cell vagotomy for duodenal ulcer deserves close observation and further consideration.", "contents": "The afferent loop syndrome. The afferent loop syndromes result from obstruction to the afferent jejunal loop. Acute ALS results from complete obstruction, usually occurs early after surgery and runs a devastatingly lethal course unless promptly treated by reoperation. In chronic ALS the obstruction is intermittent and produces a clinical syndrome from which a diagnostic histroy can usually be obtained. Although the exact incidence is unknown, it is certainly not rare, especially in antecolic Billroth II gastrectomies. Treatment consists of doing away with the afferent loop. In gastroenterostomy alone takedown of the anastomosis with a Weinberg pyloroplasty is the treatment of choice. The safest and simplest treatment for patients whose original operation was Billroth II gastrectomy is conversion to a Roux-en-Y procedure. In all cases vagotomy should be added unless previously performed. No medical treatment is available and patients with no other contraindication should have revisional surgery if symptoms are clinically significant. Both acute and chronic afferent loop syndromes should be completely prevented by appropriate choice of the initial operative procedure. The vagotomized stomach should be drained by pyloroplasty, not gastrojejunostomy. Vagotomy and antrectomy should be reconstructed with a Billroth I gastroduodenostomy. The Braun enteroanastomosis should be utilized after subtotal gastrectomy for carcinoma. The wider application of parietal cell vagotomy for duodenal ulcer deserves close observation and further consideration.", "PMID": 957782}
{"id": "pubmed23n0032_2873", "title": "Alkaline reflux gastritis.", "content": "Any surgical procedure that ablates the pyloric sphincter mechanism permits increased reflux of duodenal fluid into the stomach or gastric remnant. Although it is reported as most common with Billroth II gastrectomy, our experience indicates that reflux is nearly as frequent after Billroth I gastroduodenostomy and is not at all infrequent after pyloroplasty. The precise constituents of duodenal fluid which damage the gastric mucosa remain controversial. The best present evidence is that the bile acids are probably essential, but that one or more other constituents of duodenal content are also necessary. The clinical history differs significantly from chronic afferent loop syndrome in that the quality of pain is different, pain tends to be more continuous and less closely related to food-taking, and bile vomiting does not provide dramatic relief, often containing food due to coexistent interference with gastric emptying. Diagnosis is confirmed by gross endoscopic findings and characteristic histopathologic changes in the endoscopic biopsies. Treatment with an interposed isoperistaltic jejunal segment has been disappointing. Only four of ten patients experienced lasting relief, indicating that the relatively short 10 to 12 cm. of jejunum does not adequately prevent duodenogastric reflux. We have, therefore, shifted to the Roux-en-Y duodenal diversion implanting the afferent limb 40 cm. caudad to the gastrojejunostomy. Results have been excellent in 24 of 25 cases with prompt improvement in gastric emptying, absence of bile vomiting, progressive regression in abdominal distress and progressive improvement in nutrition. Endoscopic evaluation at three to four months has indicated marked gross improvement and striking histologic improvement in 23 of 25 cases. The question is raised whether the Roux-en-Y reconstruction should not be used primarily, particularly if both vagotomy and antrectomy are to be performed for peptic ulcer. Both the afferent loop syndrome and alkaline reflux gastritis would be prevented, and it is doubted that the incidence of marginal ulcer would increase appreciably.", "contents": "Alkaline reflux gastritis. Any surgical procedure that ablates the pyloric sphincter mechanism permits increased reflux of duodenal fluid into the stomach or gastric remnant. Although it is reported as most common with Billroth II gastrectomy, our experience indicates that reflux is nearly as frequent after Billroth I gastroduodenostomy and is not at all infrequent after pyloroplasty. The precise constituents of duodenal fluid which damage the gastric mucosa remain controversial. The best present evidence is that the bile acids are probably essential, but that one or more other constituents of duodenal content are also necessary. The clinical history differs significantly from chronic afferent loop syndrome in that the quality of pain is different, pain tends to be more continuous and less closely related to food-taking, and bile vomiting does not provide dramatic relief, often containing food due to coexistent interference with gastric emptying. Diagnosis is confirmed by gross endoscopic findings and characteristic histopathologic changes in the endoscopic biopsies. Treatment with an interposed isoperistaltic jejunal segment has been disappointing. Only four of ten patients experienced lasting relief, indicating that the relatively short 10 to 12 cm. of jejunum does not adequately prevent duodenogastric reflux. We have, therefore, shifted to the Roux-en-Y duodenal diversion implanting the afferent limb 40 cm. caudad to the gastrojejunostomy. Results have been excellent in 24 of 25 cases with prompt improvement in gastric emptying, absence of bile vomiting, progressive regression in abdominal distress and progressive improvement in nutrition. Endoscopic evaluation at three to four months has indicated marked gross improvement and striking histologic improvement in 23 of 25 cases. The question is raised whether the Roux-en-Y reconstruction should not be used primarily, particularly if both vagotomy and antrectomy are to be performed for peptic ulcer. Both the afferent loop syndrome and alkaline reflux gastritis would be prevented, and it is doubted that the incidence of marginal ulcer would increase appreciably.", "PMID": 957783}
{"id": "pubmed23n0032_2874", "title": "Gastrin and gastric surgery.", "content": "The development of the radioimmunoassay for gastrin has resulted in significant increases in our knowledge of the physiology of the stomach and antrum, and in an objective recognition of the interaction of the gastrin and vagus mechanisms. Recent identification of multiple species of gastrin in the circulation, however, raises questions as to the significance of early experimental results. Until the various aspects of gastrin and their relative contributions in the normal state and in pathologic processes are identified, the significance of gastrin levels in the evaluation of patients with uncomplicated ulcer disease is unclear. Although many investigators have attempted to correlate changes in serum gastrin levels in response to various stimuli with the completeness of vagotomy or the likelihood of recurrence, it is too early to give any clinical significance to these reports. Several points in particular seem worthy of emphasis: 1. Preoperative serum gastrin levels are currently of no value in selecting an operation for the treatment of duodenal ulcer disease. 2. The difference in serum gastrin levels in response to feeding that may be shown to exist between groups of normal subjects and duodenal ulcer patients is not a value in diagnosing ulcer disease in a specific patient, nor in differentiating duodenal ulcer from other conditions. 3. The measurement of serum gastrin levels in association with Hollander tests, while perhaps of potential future benefit, does not improve the accuracy of the Hollander test nor do results necessarily relate to vagal innervation. 4. Postoperative serum gastrin levels are increased after vagotomy. The degree of hypergastrinemia after vagotomy does not correlate with risk of ulcer recurrence. 5. Hypergastrinemia (greater than 1000 pg. per ml.) in the presence of hyperacidity is essentially pathognomonic of the Zollinger-Ellison syndrome. Calcium and secretin infusions do not add to the diagnosis if clear-cut clinical and laboratory data are present. These differential tests are of value in identifying the Zollinger-Ellison patient who has borderline serum gastrin levels and in differentiation from the syndrome of the retained antrum. 6. In a patient with a recurrent ulcer following surgery in whom a drug-induced ulcer can be excluded and gastric outlet obstruction cannot be demonstrated, a serum gastrin level may be indicated. A serum gastrin value greater than 300 pg. per ml. (normal less than 200 pg. per ml.) in a fasting morning serum sample is significantly elevated, even after vagotomy, and warrants further investigation. Provocative testing of the gastrin response to calcium and secretin should elucidate the etiology of the recurrent ulceration in this type of patient.", "contents": "Gastrin and gastric surgery. The development of the radioimmunoassay for gastrin has resulted in significant increases in our knowledge of the physiology of the stomach and antrum, and in an objective recognition of the interaction of the gastrin and vagus mechanisms. Recent identification of multiple species of gastrin in the circulation, however, raises questions as to the significance of early experimental results. Until the various aspects of gastrin and their relative contributions in the normal state and in pathologic processes are identified, the significance of gastrin levels in the evaluation of patients with uncomplicated ulcer disease is unclear. Although many investigators have attempted to correlate changes in serum gastrin levels in response to various stimuli with the completeness of vagotomy or the likelihood of recurrence, it is too early to give any clinical significance to these reports. Several points in particular seem worthy of emphasis: 1. Preoperative serum gastrin levels are currently of no value in selecting an operation for the treatment of duodenal ulcer disease. 2. The difference in serum gastrin levels in response to feeding that may be shown to exist between groups of normal subjects and duodenal ulcer patients is not a value in diagnosing ulcer disease in a specific patient, nor in differentiating duodenal ulcer from other conditions. 3. The measurement of serum gastrin levels in association with Hollander tests, while perhaps of potential future benefit, does not improve the accuracy of the Hollander test nor do results necessarily relate to vagal innervation. 4. Postoperative serum gastrin levels are increased after vagotomy. The degree of hypergastrinemia after vagotomy does not correlate with risk of ulcer recurrence. 5. Hypergastrinemia (greater than 1000 pg. per ml.) in the presence of hyperacidity is essentially pathognomonic of the Zollinger-Ellison syndrome. Calcium and secretin infusions do not add to the diagnosis if clear-cut clinical and laboratory data are present. These differential tests are of value in identifying the Zollinger-Ellison patient who has borderline serum gastrin levels and in differentiation from the syndrome of the retained antrum. 6. In a patient with a recurrent ulcer following surgery in whom a drug-induced ulcer can be excluded and gastric outlet obstruction cannot be demonstrated, a serum gastrin level may be indicated. A serum gastrin value greater than 300 pg. per ml. (normal less than 200 pg. per ml.) in a fasting morning serum sample is significantly elevated, even after vagotomy, and warrants further investigation. Provocative testing of the gastrin response to calcium and secretin should elucidate the etiology of the recurrent ulceration in this type of patient.", "PMID": 957787}
{"id": "pubmed23n0032_2875", "title": "Patient resistance and prognosis in amyotrophic lateral sclerosis.", "content": "Amyotrophic lateral sclerosis is usually considered a disease that will have a fatal termination in 1 to 3 years. A prospective study of 100 patients with this disorder revealed that 20 of them were living 5 years after the onset of their disorder. Review of other published series reveals that patients have been reported who lived for longer than 5 years and have then usually been reported as atypical cases, although the only way in which they are described as atypical is the duration of the disease. It is our assumption, based on these data as well as on additional clinical observations, that many patients with amyotrophic lateral sclerosis live for longer than 5 years and, rarely, they may have remissions of their illness. The possible significance of these observations is discussed.", "contents": "Patient resistance and prognosis in amyotrophic lateral sclerosis. Amyotrophic lateral sclerosis is usually considered a disease that will have a fatal termination in 1 to 3 years. A prospective study of 100 patients with this disorder revealed that 20 of them were living 5 years after the onset of their disorder. Review of other published series reveals that patients have been reported who lived for longer than 5 years and have then usually been reported as atypical cases, although the only way in which they are described as atypical is the duration of the disease. It is our assumption, based on these data as well as on additional clinical observations, that many patients with amyotrophic lateral sclerosis live for longer than 5 years and, rarely, they may have remissions of their illness. The possible significance of these observations is discussed.", "PMID": 957788}
{"id": "pubmed23n0032_2876", "title": "Hemoccult stool tests: false-negative results due to storage of specimens.", "content": "Stoll samples mixed with 0 to 38 mg of hemoglobin per gram of stool were tested for occult blood by the Hemoccult (HO) method when fresh and after storage for 1 to 14 days. The intensity of the HO reaction in fresh stool samples was directly related to the hemoglobin concentration, and approximately 8 mg/g was required for a positive reaction. After samples had been stored on HO cards for 4 days, the intensity of the HO reaction was decidedly less in all. Except at the highest concentrations studied, all became HO-negative or trace within 8 days. In contrast, samples of most concentrations that were stored in tightly capped containers prior to preparation of HO cards maintained a positive reaction intensity for 1 week. Nevertheless, the intensity of the HO reaction decreased significantly (P less than 0.01) with each method of storage. No false-positives were induced by storage during this portion of our study. Samples from 100 stool specimens sent to the clinical laboratory for determination of occult blood were also smeared on separate HO cards for testing immediately and after 4, 7, or 10 days of storage. Only one positive HO reaction was observed on an HO card prepared from a specimen initially classified as HO-negative. In contrast, up to 40% of the stored HO cards prepared from specimens initially classified as HO-positive were HO-negative when analyzed 4 days later.", "contents": "Hemoccult stool tests: false-negative results due to storage of specimens. Stoll samples mixed with 0 to 38 mg of hemoglobin per gram of stool were tested for occult blood by the Hemoccult (HO) method when fresh and after storage for 1 to 14 days. The intensity of the HO reaction in fresh stool samples was directly related to the hemoglobin concentration, and approximately 8 mg/g was required for a positive reaction. After samples had been stored on HO cards for 4 days, the intensity of the HO reaction was decidedly less in all. Except at the highest concentrations studied, all became HO-negative or trace within 8 days. In contrast, samples of most concentrations that were stored in tightly capped containers prior to preparation of HO cards maintained a positive reaction intensity for 1 week. Nevertheless, the intensity of the HO reaction decreased significantly (P less than 0.01) with each method of storage. No false-positives were induced by storage during this portion of our study. Samples from 100 stool specimens sent to the clinical laboratory for determination of occult blood were also smeared on separate HO cards for testing immediately and after 4, 7, or 10 days of storage. Only one positive HO reaction was observed on an HO card prepared from a specimen initially classified as HO-negative. In contrast, up to 40% of the stored HO cards prepared from specimens initially classified as HO-positive were HO-negative when analyzed 4 days later.", "PMID": 957789}
{"id": "pubmed23n0032_2877", "title": "Total respiratory resistance by forced oscillation in normal subjects.", "content": "Total respiratory resistance (Rrs) was studied by the forced oscillation technique at a frequency of 3 cps in 76 normal subjects (36 men, 40 women), ages 21 to 64 years. Slopes (equivalent to specific conductance) assuming a linear relationship with zero intercept between 1/Rrs and thoracic gas volume (TGV) were computed. In women, expiratory slopes were significantly less than inspiratory slopes; moreover, slopes decreased significantly with height and were significantly less in current smokers. For men, there were no consistently significant relationships between slopes and phase, age, height, weight, or smoking habits. Predicted resistance and estimates of the 5th and 95th percentiles of inspiratory, expiratory, and average resistance are presented for all men combined and for currently nonsmoking women. The fact that, in contrast to men, asymptomatic, current women smokers showed an elevation in Rrs when compared to nonsmoking women merits further study.", "contents": "Total respiratory resistance by forced oscillation in normal subjects. Total respiratory resistance (Rrs) was studied by the forced oscillation technique at a frequency of 3 cps in 76 normal subjects (36 men, 40 women), ages 21 to 64 years. Slopes (equivalent to specific conductance) assuming a linear relationship with zero intercept between 1/Rrs and thoracic gas volume (TGV) were computed. In women, expiratory slopes were significantly less than inspiratory slopes; moreover, slopes decreased significantly with height and were significantly less in current smokers. For men, there were no consistently significant relationships between slopes and phase, age, height, weight, or smoking habits. Predicted resistance and estimates of the 5th and 95th percentiles of inspiratory, expiratory, and average resistance are presented for all men combined and for currently nonsmoking women. The fact that, in contrast to men, asymptomatic, current women smokers showed an elevation in Rrs when compared to nonsmoking women merits further study.", "PMID": 957790}
{"id": "pubmed23n0032_2878", "title": "Vaginal flora in asymptomatic women.", "content": "Four groups of 25 asymptomatic women--pregnant, premenopausal and taking oral contraceptives, premenopausal and not taking oral contraceptives, and postmenopausal--were studied for the presence in vaginal specimens of aerobic bacteria, anaerobic bacteria, fungi, Mycoplasma, Chlamydia, herpes simplex virus, mycobacteria, and Trichomonas. No significant differences in microbial flora were found among the groups.", "contents": "Vaginal flora in asymptomatic women. Four groups of 25 asymptomatic women--pregnant, premenopausal and taking oral contraceptives, premenopausal and not taking oral contraceptives, and postmenopausal--were studied for the presence in vaginal specimens of aerobic bacteria, anaerobic bacteria, fungi, Mycoplasma, Chlamydia, herpes simplex virus, mycobacteria, and Trichomonas. No significant differences in microbial flora were found among the groups.", "PMID": 957791}
{"id": "pubmed23n0032_2879", "title": "Midline tilting between seeing and nonseeing areas in hemianopia.", "content": "The line separating the seeing from the nonseeing parts of the visual field was found to be tilted away from the expected vertical position in 24 of 200 patients diagnosed as having various types of hemianopia, including homonymous hemianopia, homonymous quadrant hemianopia, homonymous scotomata, and bitemporal hemianopia. The causes of these defects include vascular accidents, trauma, tumor, and neurosurgery for convulsive disorders. Once discovered by standard monocular field testing methods, the existence of the tilting phenomenon was distinguished from testing artifact by employing binocular fixation and red-green glasses along with red and green test objects. A difference in the overlapping fields of the two eyes was found to exist only in areas where corresponding lines between seeing and nonseeing fields were separated by a tilting away from the vertical in one or both eyes. This can occur superiorly or inferiorly with homonymous scotomata, and in bitemporal hemianopia as well as in homonymous hemianopia or quadrant hemianopia. This incongruity of fields is possibly explained by a variation in anatomic determination of crossing from noncrossing fibers at the level of the retina.", "contents": "Midline tilting between seeing and nonseeing areas in hemianopia. The line separating the seeing from the nonseeing parts of the visual field was found to be tilted away from the expected vertical position in 24 of 200 patients diagnosed as having various types of hemianopia, including homonymous hemianopia, homonymous quadrant hemianopia, homonymous scotomata, and bitemporal hemianopia. The causes of these defects include vascular accidents, trauma, tumor, and neurosurgery for convulsive disorders. Once discovered by standard monocular field testing methods, the existence of the tilting phenomenon was distinguished from testing artifact by employing binocular fixation and red-green glasses along with red and green test objects. A difference in the overlapping fields of the two eyes was found to exist only in areas where corresponding lines between seeing and nonseeing fields were separated by a tilting away from the vertical in one or both eyes. This can occur superiorly or inferiorly with homonymous scotomata, and in bitemporal hemianopia as well as in homonymous hemianopia or quadrant hemianopia. This incongruity of fields is possibly explained by a variation in anatomic determination of crossing from noncrossing fibers at the level of the retina.", "PMID": 957792}
{"id": "pubmed23n0032_2880", "title": "Ricinoleic acid effects on rabbit intestine: an ultrastructural study.", "content": "In fasting rabbits, segments of ileum and colon were perfused with ricinoleic acid. This fatty acid is the active principle of castor oil and stimulates intestinal fluid secretion. Biopsies taken from perfused segments were examined by electron microscopy after staining with lanthanum hydroxide and ruthenium red. These stains, which typically bind to extracellular surfaces, were used to assess the integrity of junctional zones between cells. In this study, ricinoleate did not alter ruthenium or lanthanum staining in junctional areas, a finding suggesting that ricinoleate does not cause fluid secretion by selectively disrupting these areas.", "contents": "Ricinoleic acid effects on rabbit intestine: an ultrastructural study. In fasting rabbits, segments of ileum and colon were perfused with ricinoleic acid. This fatty acid is the active principle of castor oil and stimulates intestinal fluid secretion. Biopsies taken from perfused segments were examined by electron microscopy after staining with lanthanum hydroxide and ruthenium red. These stains, which typically bind to extracellular surfaces, were used to assess the integrity of junctional zones between cells. In this study, ricinoleate did not alter ruthenium or lanthanum staining in junctional areas, a finding suggesting that ricinoleate does not cause fluid secretion by selectively disrupting these areas.", "PMID": 957793}
{"id": "pubmed23n0032_2881", "title": "Decreased unscheduled DNA synthesis in nondividing aged WI38 cells.", "content": "The ability of cells from young and old cultures of WI38 human fibroblasts to undergo unscheduled DNA synthesis was studied. Following inhibition of semi-conservative synthesis in media lacking arginine and containing a hydroxyurea block, cells were irradiated with u.v. light and repair replication was measured by determination of specific activity of DNA and by autoradiography. Unscheduled DNA synthetic capacity was significantly reduced in old cultures. Quantitation of silver grains revealed that this reduction was a result of decreased repair activity by individual cells, which correlated well with a similar decrease in the proportion of dividing cells in the population. This observation that unscheduled DNA synthesis is reduced in older cells is in apparent contradiction to some earlier reports, and the failure of other investigators to identify aging changes in repair capacity is discussed in relation to differences in experimental procedure.", "contents": "Decreased unscheduled DNA synthesis in nondividing aged WI38 cells. The ability of cells from young and old cultures of WI38 human fibroblasts to undergo unscheduled DNA synthesis was studied. Following inhibition of semi-conservative synthesis in media lacking arginine and containing a hydroxyurea block, cells were irradiated with u.v. light and repair replication was measured by determination of specific activity of DNA and by autoradiography. Unscheduled DNA synthetic capacity was significantly reduced in old cultures. Quantitation of silver grains revealed that this reduction was a result of decreased repair activity by individual cells, which correlated well with a similar decrease in the proportion of dividing cells in the population. This observation that unscheduled DNA synthesis is reduced in older cells is in apparent contradiction to some earlier reports, and the failure of other investigators to identify aging changes in repair capacity is discussed in relation to differences in experimental procedure.", "PMID": 957802}
{"id": "pubmed23n0032_2882", "title": "Ovarian development in athymic nude mice V. The effects of PMSG upon the numbers and growth of follicles in the early juvenile ovary.", "content": "The composition and growth patterns of the ovarian follicle population have been determined in early juvenile athymic nude mice and their phenotypically normal littermates. Nude ovaries contained significantly more primordial follicles and fewer trilaminar follicles at 10 days of age. The rate of growth of the oocyte nucleolus was significantly less than in control ovaries. Treatment with PMSG from days 7 to 9 restored the rate of oocyte nucleolar growth in nudes to control levels. PMSG also increased the number of follicles commencing growth in both nudes and controls and the differences in the numbers of primordial and trilaminar follicles were no longer detectable. The data demonstrate that abnormalities in the ovarian follicle population of the nude are detectable as early as the 10th day of life and can be reversed by treatment with exogenous gonadotrophin. These results are discussed in relation to the role of the thymus gland in ovarian development and the competence of the early juvenile ovary to respond to gonadotrophin.", "contents": "Ovarian development in athymic nude mice V. The effects of PMSG upon the numbers and growth of follicles in the early juvenile ovary. The composition and growth patterns of the ovarian follicle population have been determined in early juvenile athymic nude mice and their phenotypically normal littermates. Nude ovaries contained significantly more primordial follicles and fewer trilaminar follicles at 10 days of age. The rate of growth of the oocyte nucleolus was significantly less than in control ovaries. Treatment with PMSG from days 7 to 9 restored the rate of oocyte nucleolar growth in nudes to control levels. PMSG also increased the number of follicles commencing growth in both nudes and controls and the differences in the numbers of primordial and trilaminar follicles were no longer detectable. The data demonstrate that abnormalities in the ovarian follicle population of the nude are detectable as early as the 10th day of life and can be reversed by treatment with exogenous gonadotrophin. These results are discussed in relation to the role of the thymus gland in ovarian development and the competence of the early juvenile ovary to respond to gonadotrophin.", "PMID": 957803}
{"id": "pubmed23n0032_2883", "title": "Changes in the pattern of RNA synthesis in mouse limb cartilage during early development.", "content": "Patterns of RNA synthesis using tritiated uridine were studied in organ cultures of mouse embryonic limb cartilage and epiphyseal cartilage from mice 5 days of age. The cold phenol-sodium dodecyl sulfate extracted RNA was characterized by methylated albumin kieselguhr chromatography and polyacrylamide gel electrophoresis. The general pattern of synthesis indicates the persistence of primarily low molecular weight tRNA and a diminishing synthesis of rRNA in the older cartilage. Data show that the increased radioactivity in the low molecular weight fraction is not due to the accumulation of rRNA breakdown products. These findings are discussed in relation to other age-related studies on RNA biosynthesis.", "contents": "Changes in the pattern of RNA synthesis in mouse limb cartilage during early development. Patterns of RNA synthesis using tritiated uridine were studied in organ cultures of mouse embryonic limb cartilage and epiphyseal cartilage from mice 5 days of age. The cold phenol-sodium dodecyl sulfate extracted RNA was characterized by methylated albumin kieselguhr chromatography and polyacrylamide gel electrophoresis. The general pattern of synthesis indicates the persistence of primarily low molecular weight tRNA and a diminishing synthesis of rRNA in the older cartilage. Data show that the increased radioactivity in the low molecular weight fraction is not due to the accumulation of rRNA breakdown products. These findings are discussed in relation to other age-related studies on RNA biosynthesis.", "PMID": 957805}
{"id": "pubmed23n0032_2884", "title": "Urine sodium, potassium and osmolality in two rat strains selected for their different ethanol preferences.", "content": "Urine sodium, potassium and osmolality were investigated during water and ethanol diuresis in two rat strains, AA and ANA, which drink voluntarily different amounts of ethanol. At the start of each experiment the rats were in a positive water balance. During ethanol intoxication the AA strain excreted more urine than the ANA strain. In ethanol experiments the osmolality of the urine was higher in the AA strain than in the ANA strain. With ethanol amounts of 2.4 g/kg body weight and 4.8 g/kg of body weight, urinary sodium and potassium output was greater in AA rats than ANA rats. When only water was introduced urine volumes and the excretion of sodium and potassium during 180 min were greater in ANA males than in AA males.", "contents": "Urine sodium, potassium and osmolality in two rat strains selected for their different ethanol preferences. Urine sodium, potassium and osmolality were investigated during water and ethanol diuresis in two rat strains, AA and ANA, which drink voluntarily different amounts of ethanol. At the start of each experiment the rats were in a positive water balance. During ethanol intoxication the AA strain excreted more urine than the ANA strain. In ethanol experiments the osmolality of the urine was higher in the AA strain than in the ANA strain. With ethanol amounts of 2.4 g/kg body weight and 4.8 g/kg of body weight, urinary sodium and potassium output was greater in AA rats than ANA rats. When only water was introduced urine volumes and the excretion of sodium and potassium during 180 min were greater in ANA males than in AA males.", "PMID": 957811}
{"id": "pubmed23n0032_2885", "title": "A lack of correlation between decline in growth capacity and nuclear RNA synthesising activity in ageing human embryo cells in culture.", "content": "A reduced proliferative capacity has been demonstrated between young (passage 5/24) and middle (passage 17/24) aged human embryo diploid fibroblast cultures. This difference was noted in both logarithmically growing and plateau-phase cultures in terms of growth curves, DNA content per dish and ability to incorporate 3H-thymidine. This reduction in growth potential is not accompanied by any reduction in chromatin template activity, determined in nuclear \"ghost\" monolayer, using either the endogenous polymerase or an exogenous bacterial enzyme. Therefore the declining division potential as these cells pass from young to middle passage is unlikely to occur as an expression of alterations in nuclear RNA synthesis. It is, however, possible that the apparently continuous decline in growth potential with age might be caused by different events at various stages of the cells's lifespan.", "contents": "A lack of correlation between decline in growth capacity and nuclear RNA synthesising activity in ageing human embryo cells in culture. A reduced proliferative capacity has been demonstrated between young (passage 5/24) and middle (passage 17/24) aged human embryo diploid fibroblast cultures. This difference was noted in both logarithmically growing and plateau-phase cultures in terms of growth curves, DNA content per dish and ability to incorporate 3H-thymidine. This reduction in growth potential is not accompanied by any reduction in chromatin template activity, determined in nuclear \"ghost\" monolayer, using either the endogenous polymerase or an exogenous bacterial enzyme. Therefore the declining division potential as these cells pass from young to middle passage is unlikely to occur as an expression of alterations in nuclear RNA synthesis. It is, however, possible that the apparently continuous decline in growth potential with age might be caused by different events at various stages of the cells's lifespan.", "PMID": 957804}
{"id": "pubmed23n0032_2886", "title": "Increased cholesterol content and esterification in rabbit aortic medial cells cultured in hyperlipidemic serum.", "content": "Cells of early atherosclerotic lesions have an increased content of cholesteryl esters and enhanced rate of cholesterol esterification. The present study was carried out to elucidate whether these changes could be reproduced in a purely in vitro system. Cultures of rabbit aortic medial cells were incubated in a medium containing 10% of either normal rabbit serum (control cells) or serum from rabbits with cholesterol-induced hyperlipidemia (hyperlipidemic cells). The incorporation rate of [1-14C]oleate into cellular cholesteryl esters increased two- to threefold after four hours' incubation with hyperlipidemic serum and remained elevated during the rest of the eight days' study. After seven days' incubation the hyperlipidemic cells incorporated significantly more (+13%) oleate into triglycerides and significantly less (-15%) oleate into phospholipids than did the control cells. Cholesteryl ester content of the hyperlipidemic cells rose steeply for about two days, with a slower rise thereafter. In hyperlipidemic cells the esterified cholesterol content was significantly higher (315 ng/mug DNA) than in the control cells (79 ng/mug DNA). Hyperlipidemic cells showed a slow but significant rise of free cholesterol as well (from 1.15 mug/mug DNA in control to 1.50 mug/mug DNA). The results indicate that lipid changes resembling those in early atherosclerotic lesions can be brought about in vitro 0y treatment of aortic cells with hyperlipidemic serum.", "contents": "Increased cholesterol content and esterification in rabbit aortic medial cells cultured in hyperlipidemic serum. Cells of early atherosclerotic lesions have an increased content of cholesteryl esters and enhanced rate of cholesterol esterification. The present study was carried out to elucidate whether these changes could be reproduced in a purely in vitro system. Cultures of rabbit aortic medial cells were incubated in a medium containing 10% of either normal rabbit serum (control cells) or serum from rabbits with cholesterol-induced hyperlipidemia (hyperlipidemic cells). The incorporation rate of [1-14C]oleate into cellular cholesteryl esters increased two- to threefold after four hours' incubation with hyperlipidemic serum and remained elevated during the rest of the eight days' study. After seven days' incubation the hyperlipidemic cells incorporated significantly more (+13%) oleate into triglycerides and significantly less (-15%) oleate into phospholipids than did the control cells. Cholesteryl ester content of the hyperlipidemic cells rose steeply for about two days, with a slower rise thereafter. In hyperlipidemic cells the esterified cholesterol content was significantly higher (315 ng/mug DNA) than in the control cells (79 ng/mug DNA). Hyperlipidemic cells showed a slow but significant rise of free cholesterol as well (from 1.15 mug/mug DNA in control to 1.50 mug/mug DNA). The results indicate that lipid changes resembling those in early atherosclerotic lesions can be brought about in vitro 0y treatment of aortic cells with hyperlipidemic serum.", "PMID": 957812}
{"id": "pubmed23n0032_2887", "title": "Serum parathyroid hormone levels and serum calcium levels from birth to senescence.", "content": "1. Calcium and immunoreactive parathyroid hormone (iPTH) levels were measured in sera of 1334 normal subjects ranging from newborn to over 80 years of age. Albumin was measured in samples from the population of adults, which was 80% white, 15% black and 5% oriental. 2. Serum calcium and iPTH levels in children tended to be higher in the first three years of age; no sex differences were noted. Values for serum calcium and iPTH were higher in children than in adults. 3. Serum calcium, iPTH and albumin showed more variation in groups of white, black and oriental women than in similar groups of men. In white females the mean serum calcium remained fairly constant until age 60, whereas in black women it rose steadily from age 20-29 until age 50-59. Serum iPTH levels were lower in black women than in white women and usually were not measurable in oriental women. 4. In men (white, black and oriental) there was a steady decrease in mean serum calcium with age, and iPTH levels were not different from those observed in white women. 5. Although the number of samples from oriental women was small, the serum calcium was consistently lower and serum albumin was constantly higher than in white or black women, and iPTH levels usually were unmeasurable.", "contents": "Serum parathyroid hormone levels and serum calcium levels from birth to senescence. 1. Calcium and immunoreactive parathyroid hormone (iPTH) levels were measured in sera of 1334 normal subjects ranging from newborn to over 80 years of age. Albumin was measured in samples from the population of adults, which was 80% white, 15% black and 5% oriental. 2. Serum calcium and iPTH levels in children tended to be higher in the first three years of age; no sex differences were noted. Values for serum calcium and iPTH were higher in children than in adults. 3. Serum calcium, iPTH and albumin showed more variation in groups of white, black and oriental women than in similar groups of men. In white females the mean serum calcium remained fairly constant until age 60, whereas in black women it rose steadily from age 20-29 until age 50-59. Serum iPTH levels were lower in black women than in white women and usually were not measurable in oriental women. 4. In men (white, black and oriental) there was a steady decrease in mean serum calcium with age, and iPTH levels were not different from those observed in white women. 5. Although the number of samples from oriental women was small, the serum calcium was consistently lower and serum albumin was constantly higher than in white or black women, and iPTH levels usually were unmeasurable.", "PMID": 957806}
{"id": "pubmed23n0032_2888", "title": "Molecular turnover numbers of different forms of mitochondrial monoamine oxidase in rat.", "content": "Molecular turnover numbers of the different forms of monoamine oxidase in rat liver were estimated for serotonin, tyramine and beta-phenylethylamine by titration with irreversible inhibitors. The 'A' form was found to have a much higher turnover number for serotonin and a lower turnover number for beta-phenylethylamine than the 'B' form, while the turnover numbers for tyramine were in the same order of magnitude for both forms. The monoamine oxidase in the liver was found to have a significantly higher molecular turnover for tyramine and beta-phenylethylamine than that in the brain, heart and kidney. It was calculated that the 'A' form of monoamine oxidase amounted to about 20% and the 'B' form to about 80% of the total amount of monoamine oxidase in the rat liver mitochondrial preparation. The number of molecules per mitochondrion was also calculated.", "contents": "Molecular turnover numbers of different forms of mitochondrial monoamine oxidase in rat. Molecular turnover numbers of the different forms of monoamine oxidase in rat liver were estimated for serotonin, tyramine and beta-phenylethylamine by titration with irreversible inhibitors. The 'A' form was found to have a much higher turnover number for serotonin and a lower turnover number for beta-phenylethylamine than the 'B' form, while the turnover numbers for tyramine were in the same order of magnitude for both forms. The monoamine oxidase in the liver was found to have a significantly higher molecular turnover for tyramine and beta-phenylethylamine than that in the brain, heart and kidney. It was calculated that the 'A' form of monoamine oxidase amounted to about 20% and the 'B' form to about 80% of the total amount of monoamine oxidase in the rat liver mitochondrial preparation. The number of molecules per mitochondrion was also calculated.", "PMID": 957813}
{"id": "pubmed23n0032_2889", "title": "On the visualization of central dopamine and noradrenaline nerve terminals in cryostat sections.", "content": "Brain tissue from rats perfused with a mixture of glyoxylic acid and formaldehyde was frozen and sectioned in a cryostat. The cryostat sections were incubated in glyoxylic acid, dried and reacted with formaldehyde vapor. Using this technique cortical noradrenaline and dopamine containing nerve terminals could be visualized with high sensitivity in a reproducible way. The importance of several critical parameters was evaluated and it was found that the incubation of the cryostat sections in glyoxylic acid effectively inhibited the diffusion of catecholamines, particularly dopamine. Thus the fixation effect of glyoxylic acid was considered important besides the fluorophore formation. This modification of the Falck-Hillarp fluorescence technique is rapid, simple and reproducible and might be of value especially for quantitative measurements of the fluorescence.", "contents": "On the visualization of central dopamine and noradrenaline nerve terminals in cryostat sections. Brain tissue from rats perfused with a mixture of glyoxylic acid and formaldehyde was frozen and sectioned in a cryostat. The cryostat sections were incubated in glyoxylic acid, dried and reacted with formaldehyde vapor. Using this technique cortical noradrenaline and dopamine containing nerve terminals could be visualized with high sensitivity in a reproducible way. The importance of several critical parameters was evaluated and it was found that the incubation of the cryostat sections in glyoxylic acid effectively inhibited the diffusion of catecholamines, particularly dopamine. Thus the fixation effect of glyoxylic acid was considered important besides the fluorophore formation. This modification of the Falck-Hillarp fluorescence technique is rapid, simple and reproducible and might be of value especially for quantitative measurements of the fluorescence.", "PMID": 957814}
{"id": "pubmed23n0032_2890", "title": "The potential of the teaching hospital for the development of prepaid group practices.", "content": "This paper deals with the development of a hospital based prepaid group practice at Long Island Jewish-Hillside Medical Center. The paper provides an historical perspective of the factors leading to the decision to develop such a Medical Group practice, the difficulties encountered in obtaining conceptual approval from the Medical Center's Board of Trustees and its medical staff, the negotiations between the Medical Center and Blue Cross-Blue Shield of Greater New York as the insurance company which agreed to market the program and to subsidize it in its developmental stage, factors relating to the contractual negotiations between the Medical Center and Blue Cross, the key elements of the negotiated contract from the standpoint of the prepaid group, and the nature of the subsidies provided to ensure a healthy fiscal beginning. The paper highlights the methods used in achieving the desired goals and the development of the Group, sets forth the resistance which other, similar hospitals might anticipate in undertaking such a venture of their own, and highlights the nature and the degree of commitment necessary to bring such a program into being.", "contents": "The potential of the teaching hospital for the development of prepaid group practices. This paper deals with the development of a hospital based prepaid group practice at Long Island Jewish-Hillside Medical Center. The paper provides an historical perspective of the factors leading to the decision to develop such a Medical Group practice, the difficulties encountered in obtaining conceptual approval from the Medical Center's Board of Trustees and its medical staff, the negotiations between the Medical Center and Blue Cross-Blue Shield of Greater New York as the insurance company which agreed to market the program and to subsidize it in its developmental stage, factors relating to the contractual negotiations between the Medical Center and Blue Cross, the key elements of the negotiated contract from the standpoint of the prepaid group, and the nature of the subsidies provided to ensure a healthy fiscal beginning. The paper highlights the methods used in achieving the desired goals and the development of the Group, sets forth the resistance which other, similar hospitals might anticipate in undertaking such a venture of their own, and highlights the nature and the degree of commitment necessary to bring such a program into being.", "PMID": 957815}
{"id": "pubmed23n0032_2891", "title": "Integrating mental health services into primary medical care.", "content": "Primary care clinicians occupy a strategic position in relation to the emotional problems of their patients. Integrating mental health and primary medical services promotes available, coordinated, accessible, and less stigmatizing treatment by recognizing an indivisibility of the total person in illness and in health. Federal efforts to encourage Health Maintenance Organization (HMO) development as part of a national health program prompts serious attention to organizational arrangements for developing such an integrated program for medical-mental health care. We have found a team collaborative model in which mental health providers are members of a primary care team to be useful and promising. Supportive services are provided on a continuing basis through patterned relationships. Shared responsibility for patient care between physicians, nurse practitioners, physician assistants, and mental health workers provides built-in peer review and encourages intrateam consultation.", "contents": "Integrating mental health services into primary medical care. Primary care clinicians occupy a strategic position in relation to the emotional problems of their patients. Integrating mental health and primary medical services promotes available, coordinated, accessible, and less stigmatizing treatment by recognizing an indivisibility of the total person in illness and in health. Federal efforts to encourage Health Maintenance Organization (HMO) development as part of a national health program prompts serious attention to organizational arrangements for developing such an integrated program for medical-mental health care. We have found a team collaborative model in which mental health providers are members of a primary care team to be useful and promising. Supportive services are provided on a continuing basis through patterned relationships. Shared responsibility for patient care between physicians, nurse practitioners, physician assistants, and mental health workers provides built-in peer review and encourages intrateam consultation.", "PMID": 957816}
{"id": "pubmed23n0032_2892", "title": "Patient knowledge and nurse role strain in three hospital settings.", "content": "The relationship between patient medical and hospital knowledge and nurse role strain is examined within three contrasting types of general hospital. The nature of this relationship was found to differ greatly within each of the three hospitals. Interpretations of these findings focus upon differences in organizational structure and climate, staff orientation, and type of clientele group. These data suggest that cuation should accompany attempts to generalize across different types of hospitals when questions relevant to the patient role are posed.", "contents": "Patient knowledge and nurse role strain in three hospital settings. The relationship between patient medical and hospital knowledge and nurse role strain is examined within three contrasting types of general hospital. The nature of this relationship was found to differ greatly within each of the three hospitals. Interpretations of these findings focus upon differences in organizational structure and climate, staff orientation, and type of clientele group. These data suggest that cuation should accompany attempts to generalize across different types of hospitals when questions relevant to the patient role are posed.", "PMID": 957817}
{"id": "pubmed23n0032_2893", "title": "The contractual model for prison health care.", "content": "In New York City, the Department of Health is responsible for providing health services to 8 correctional facilities which receive 60,000 admissions annually. A large component of this prison health system is a contract with Montefiore Hospital, a voluntary institution, to provide medical services to the population on Rikers Island, a penal complex. This contractual agreement is unique in that an operating entity, Monefiore Hospital, has agreed to provide a \"package\" of health services to a defined prisoner population. The City agreed to reimbruse to the Hospital for the three year term of the contract, a total amount not to exceed +11.7 million. Because of delays in renovation of the prison facilities, for the majority of the period of the three year contract, only two prisons were served via the contractual route. Since 1973, the program has provided a range of services including screening of new prisoners, primary, emergency, infirmary and limited speciality care. Major improvements in laboratory and radiology services were implemented. The early experience with this program indicates that in this setting the contractual model possesses a number of distinct advantages over the direct delivery of prison health services. Disadvantages include a significantly higher cost.", "contents": "The contractual model for prison health care. In New York City, the Department of Health is responsible for providing health services to 8 correctional facilities which receive 60,000 admissions annually. A large component of this prison health system is a contract with Montefiore Hospital, a voluntary institution, to provide medical services to the population on Rikers Island, a penal complex. This contractual agreement is unique in that an operating entity, Monefiore Hospital, has agreed to provide a \"package\" of health services to a defined prisoner population. The City agreed to reimbruse to the Hospital for the three year term of the contract, a total amount not to exceed +11.7 million. Because of delays in renovation of the prison facilities, for the majority of the period of the three year contract, only two prisons were served via the contractual route. Since 1973, the program has provided a range of services including screening of new prisoners, primary, emergency, infirmary and limited speciality care. Major improvements in laboratory and radiology services were implemented. The early experience with this program indicates that in this setting the contractual model possesses a number of distinct advantages over the direct delivery of prison health services. Disadvantages include a significantly higher cost.", "PMID": 957818}
{"id": "pubmed23n0032_2894", "title": "Health care for elderly Americans: evaluation of an adult day health care model.", "content": "This study was designed to analyze the cost and effectiveness of a day care program for the elderly. An experimental group of adult day care participants was compared with a control group of elderly people living in the community. The two groups were evaluated in terms of levels of physical and emotional functioning, behavioral independence in activities of daily living, institutionalization, interpersonal relationships, and re-establishment of life styles. Statistical tests of difference were used to determine if the two groups had improved or were maintained over the six-month study period (November 1973 to March 1974). The experimental group showed improvement and differed significantly from the control group in the areas of emotional functioning, self-maintenance, and interpersonal relationships. No significant differences were found in satisfaction with life styles for either group. The experimental group was somewhat, but not significantly, deficient in independence in daily living activities. Composite analysis of six combined scales indicated that the experimental group inproved in level of functioning over the study period while the control group regressed. Cost analysis of the Lexington Center indicates that adult day care services cost +12.99 per participant per day.", "contents": "Health care for elderly Americans: evaluation of an adult day health care model. This study was designed to analyze the cost and effectiveness of a day care program for the elderly. An experimental group of adult day care participants was compared with a control group of elderly people living in the community. The two groups were evaluated in terms of levels of physical and emotional functioning, behavioral independence in activities of daily living, institutionalization, interpersonal relationships, and re-establishment of life styles. Statistical tests of difference were used to determine if the two groups had improved or were maintained over the six-month study period (November 1973 to March 1974). The experimental group showed improvement and differed significantly from the control group in the areas of emotional functioning, self-maintenance, and interpersonal relationships. No significant differences were found in satisfaction with life styles for either group. The experimental group was somewhat, but not significantly, deficient in independence in daily living activities. Composite analysis of six combined scales indicated that the experimental group inproved in level of functioning over the study period while the control group regressed. Cost analysis of the Lexington Center indicates that adult day care services cost +12.99 per participant per day.", "PMID": 957819}
{"id": "pubmed23n0032_2895", "title": "[The emptying process of the residual stomach after various methods of anastomoses (author's transl)].", "content": "Similar to the high pressure zone of the lower esophageal segment (LES) gastric emptying results from alternating intraluminal pressure in the antrum, pylorus and duodenum. Alterations of this antro-duodenal pressure zone depend on hormonal and nervous control mechanisms and are regulated by intraduodenal localized pH-, osmo- and fat-receptors. Experimental investigations in partially gastrectomized dogs emphasize the important role of the duodenum for gastric emptying. After resection of the lower half of the stomach and reconstruction of the orthograde passage between the gastric remnant and the duodenum by gastroduodenostomy (b i-anastomosis), a hormonally controlled gastric emptying comparable to that of the intact organ can be noticed.", "contents": "[The emptying process of the residual stomach after various methods of anastomoses (author's transl)]. Similar to the high pressure zone of the lower esophageal segment (LES) gastric emptying results from alternating intraluminal pressure in the antrum, pylorus and duodenum. Alterations of this antro-duodenal pressure zone depend on hormonal and nervous control mechanisms and are regulated by intraduodenal localized pH-, osmo- and fat-receptors. Experimental investigations in partially gastrectomized dogs emphasize the important role of the duodenum for gastric emptying. After resection of the lower half of the stomach and reconstruction of the orthograde passage between the gastric remnant and the duodenum by gastroduodenostomy (b i-anastomosis), a hormonally controlled gastric emptying comparable to that of the intact organ can be noticed.", "PMID": 957837}
{"id": "pubmed23n0032_2896", "title": "[The importance of the Musculus canalis ani for continence and anorectal diseases (author's transl)].", "content": "The functional morphology of the M. canalis ani is described. Hitherto this muscle has not been studied in detail. The M. canalis ani is located inside of the M. sphincter ani internus and reaches through the spatium submucosum et subcutaneum of the analcanal. This muscle is part of a described \"organ of continence\". Its importance in the course of anorectal disease is shown.", "contents": "[The importance of the Musculus canalis ani for continence and anorectal diseases (author's transl)]. The functional morphology of the M. canalis ani is described. Hitherto this muscle has not been studied in detail. The M. canalis ani is located inside of the M. sphincter ani internus and reaches through the spatium submucosum et subcutaneum of the analcanal. This muscle is part of a described \"organ of continence\". Its importance in the course of anorectal disease is shown.", "PMID": 957838}
{"id": "pubmed23n0032_2897", "title": "[Endoscopical and radiological studies after papillotomy (author's transl)].", "content": "The paper deals with a controlled study concerning 36 patients who underwent surgical papillotomy. Cholangiographic examinations were performed by infusion and endoscopic retrograde instillation, in ten patients additional percutaneaus \"blind\" biopsy of the liver. Some new remarkable details could be evaluated at the papilla Vateri itself as definite anatomic alteration after papillotomy. Planimetric studies could be added on special details because of the good x-ray-contrasts by endoscopic filling of the biliary tract. With some special reserves the results after surgical papillotomy in 30 controlled persons can be classified as good.", "contents": "[Endoscopical and radiological studies after papillotomy (author's transl)]. The paper deals with a controlled study concerning 36 patients who underwent surgical papillotomy. Cholangiographic examinations were performed by infusion and endoscopic retrograde instillation, in ten patients additional percutaneaus \"blind\" biopsy of the liver. Some new remarkable details could be evaluated at the papilla Vateri itself as definite anatomic alteration after papillotomy. Planimetric studies could be added on special details because of the good x-ray-contrasts by endoscopic filling of the biliary tract. With some special reserves the results after surgical papillotomy in 30 controlled persons can be classified as good.", "PMID": 957839}
{"id": "pubmed23n0032_2898", "title": "[New method for security by colon anastomoses (author's transl)].", "content": "After examinaning the vascular supply of the parietal peritoneum helved peritoneal flaps were fixed in wound dehiscent colon anastomoses. If the vascular supply to the peritoneal flap was sufficient all animals survived without signs of stenosis. It was proved by microangiographic studies that there was an additional vascular supply to the anastomosed colon by the artery of the peritoneal flap.", "contents": "[New method for security by colon anastomoses (author's transl)]. After examinaning the vascular supply of the parietal peritoneum helved peritoneal flaps were fixed in wound dehiscent colon anastomoses. If the vascular supply to the peritoneal flap was sufficient all animals survived without signs of stenosis. It was proved by microangiographic studies that there was an additional vascular supply to the anastomosed colon by the artery of the peritoneal flap.", "PMID": 957840}
{"id": "pubmed23n0032_2899", "title": "[Postoperative enterocutaneous fistulae of the small gut (author's transl)].", "content": "Postoperative enterocutaneous fistulae are fortunately rate (1 : 1000), but they carry a mortality of about 50%. The authors analyse the development and treatment of fistulae in 30 patients. This depends on whether the cause of the fistula is connected with an anastomosis or not, on a variety of pathologic-anatomical criteria and in particular on the amount of fluid lost daily. If this loss is less than 500 ml/24 h the fistula can be regarded as relatively benign and in all probability conservative treatment alone will succeed. Fistulae with a fluid loss of more than 500 ml/24 h have a worse prognosis and require a combined conservative and surgical therapy. The key to this problem is the optimal time for surgical intervention, it lies around the 4th week. In case an early operation is necessary one must be prepared for an enterostomy or by-pass procedure.", "contents": "[Postoperative enterocutaneous fistulae of the small gut (author's transl)]. Postoperative enterocutaneous fistulae are fortunately rate (1 : 1000), but they carry a mortality of about 50%. The authors analyse the development and treatment of fistulae in 30 patients. This depends on whether the cause of the fistula is connected with an anastomosis or not, on a variety of pathologic-anatomical criteria and in particular on the amount of fluid lost daily. If this loss is less than 500 ml/24 h the fistula can be regarded as relatively benign and in all probability conservative treatment alone will succeed. Fistulae with a fluid loss of more than 500 ml/24 h have a worse prognosis and require a combined conservative and surgical therapy. The key to this problem is the optimal time for surgical intervention, it lies around the 4th week. In case an early operation is necessary one must be prepared for an enterostomy or by-pass procedure.", "PMID": 957841}
{"id": "pubmed23n0032_2900", "title": "Histopathologic correlates of otoneurologic manifestations following head trauma.", "content": "The clinical course of patients dying of head trauma and the physiologic evaluation of audition in guinea pigs subjected to several forms of head trauma are correlated with the histopathologic findings in the brains and temporal bones. In the patients there was wide spread hemorrhage, edema, disorganization and disruption of neural tissue. Loss of Purkinje cells and other neural elements was prominent. The temporal bone findings in the patients included laceration of and hemorrhage into the VIIIth nerve as well as bleeding into the scala tympani. The membranous labyrinths demonstrated no specific changes secondary to the trauma. The guinea pigs developed a central form of hearing loss with elevated thresholds for evoked responses from the inferior colliculus and normal AC cochlear potentials. In the animals hemorrhage in the substance of the brain, necrosis of neural tissue, edema, disorganization and disruption were prominent. Although there was extravasation of blood in the tympanic and vestibular scalae, the membranous structures of the inner ears were well preserved.", "contents": "Histopathologic correlates of otoneurologic manifestations following head trauma. The clinical course of patients dying of head trauma and the physiologic evaluation of audition in guinea pigs subjected to several forms of head trauma are correlated with the histopathologic findings in the brains and temporal bones. In the patients there was wide spread hemorrhage, edema, disorganization and disruption of neural tissue. Loss of Purkinje cells and other neural elements was prominent. The temporal bone findings in the patients included laceration of and hemorrhage into the VIIIth nerve as well as bleeding into the scala tympani. The membranous labyrinths demonstrated no specific changes secondary to the trauma. The guinea pigs developed a central form of hearing loss with elevated thresholds for evoked responses from the inferior colliculus and normal AC cochlear potentials. In the animals hemorrhage in the substance of the brain, necrosis of neural tissue, edema, disorganization and disruption were prominent. Although there was extravasation of blood in the tympanic and vestibular scalae, the membranous structures of the inner ears were well preserved.", "PMID": 957842}
{"id": "pubmed23n0032_2901", "title": "Inner ear decompression sickness.", "content": "With recent increases in commercial, military, and sport diving to deeper depths, inner ear injuries during such exposures have been encountered more frequently and noted during several phases of diving: during compression, at stable deep depths, with excessive noise exposure in diving, and during decompression. The pathophysiology of these injuries differs, depending upon the phase of diving in which the injuries occur. In this report, 23 cases of hearing loss, tinnitus, and/or vertigo occurring during or shortly after decompression are presented. Thirteen of these cases occurred in helium-oxygen dives involving a change to air during the latter stages of decompression. A significant correlation is present between prompt recompression treatment, relief of symptoms, and lack of residual deficits. Current knowledge indicates that the management of otologic decompression sickness should include: 1. prompt recompression to at least 99 feet deeper than the symptom onset depth; 2. recompression using the previous helium-oxygen mixture when the injuries occur during or shortly after a switch from helium-oxygen to air during the latter stages of decompression; 3. the use of parenteral diazepam for symptom relief and cyclic inhalations of oxygen enriched treatment gases; and 4. the avoidance of further diving by divers who exhibit permanent inner ear injuries after the acute symptoms have subsided.", "contents": "Inner ear decompression sickness. With recent increases in commercial, military, and sport diving to deeper depths, inner ear injuries during such exposures have been encountered more frequently and noted during several phases of diving: during compression, at stable deep depths, with excessive noise exposure in diving, and during decompression. The pathophysiology of these injuries differs, depending upon the phase of diving in which the injuries occur. In this report, 23 cases of hearing loss, tinnitus, and/or vertigo occurring during or shortly after decompression are presented. Thirteen of these cases occurred in helium-oxygen dives involving a change to air during the latter stages of decompression. A significant correlation is present between prompt recompression treatment, relief of symptoms, and lack of residual deficits. Current knowledge indicates that the management of otologic decompression sickness should include: 1. prompt recompression to at least 99 feet deeper than the symptom onset depth; 2. recompression using the previous helium-oxygen mixture when the injuries occur during or shortly after a switch from helium-oxygen to air during the latter stages of decompression; 3. the use of parenteral diazepam for symptom relief and cyclic inhalations of oxygen enriched treatment gases; and 4. the avoidance of further diving by divers who exhibit permanent inner ear injuries after the acute symptoms have subsided.", "PMID": 957843}
{"id": "pubmed23n0032_2902", "title": "The knotted wire in stapes surgery: one possible factor in postoperative sensori-neural hearing loss.", "content": "In four instances of revision stapedectomy in patients with sensori-neural hearing loss, with or without dizziness, a wire prosthesis was found with an extention beyond the knot which could impinge on the contents of the vestibule. Four commercially manufactured knotted wire prostheses were examined, and the end of three was found projecting beyond the knot. The wire piercing the membranous labyrinth appears to be one of the factors in causing further sensori-neural hearing loss after stapedectomy. A wire loop or piston prosthesis should be considered to eliminate the hazard of a trailing end of a knotted wire in stapes surgery.", "contents": "The knotted wire in stapes surgery: one possible factor in postoperative sensori-neural hearing loss. In four instances of revision stapedectomy in patients with sensori-neural hearing loss, with or without dizziness, a wire prosthesis was found with an extention beyond the knot which could impinge on the contents of the vestibule. Four commercially manufactured knotted wire prostheses were examined, and the end of three was found projecting beyond the knot. The wire piercing the membranous labyrinth appears to be one of the factors in causing further sensori-neural hearing loss after stapedectomy. A wire loop or piston prosthesis should be considered to eliminate the hazard of a trailing end of a knotted wire in stapes surgery.", "PMID": 957844}
{"id": "pubmed23n0032_2903", "title": "Fractures of the edentulous maxilla and mandible.", "content": "An evaluation and treatment of fracture of the edentulous maxilla and/or mandible is presented. The Le-Fort classification of maxillary fractures is the foundation for the program of care. Immobilization techniques are based upon the support of a fracture by suspension from the adjacent stable segments to the modified prosthesis. The mandibular fractures are repaired by interosseous wire fixation in the body and symphysis region, the other fracture sites are supported by circumferential wiring to the prosthesis for immobilization. General principles of time of repair, diet, types of healing factors are reviewed.", "contents": "Fractures of the edentulous maxilla and mandible. An evaluation and treatment of fracture of the edentulous maxilla and/or mandible is presented. The Le-Fort classification of maxillary fractures is the foundation for the program of care. Immobilization techniques are based upon the support of a fracture by suspension from the adjacent stable segments to the modified prosthesis. The mandibular fractures are repaired by interosseous wire fixation in the body and symphysis region, the other fracture sites are supported by circumferential wiring to the prosthesis for immobilization. General principles of time of repair, diet, types of healing factors are reviewed.", "PMID": 957845}
{"id": "pubmed23n0032_2904", "title": "Cochlear implants: technical and clinical implications.", "content": "Recent investigations using animal models and human subjects have helped to define the limits which are achievable in terms of information transfer with single-channel electrical stimulation of the auditory nerve. These studies are reviewed, and presented as a context for a description of the development of an implantable multiple-electrode multiplexing system which may provide control for several stimulus channels. While such a device may enable the stimulation of small segments of the auditory nerve independently, it does not contain any means of complex stimulus analysis and recording. This latter aspect of prosthesis development remains as the most formidable problem, from a technical viewpoint, that stands in the way of any significant progress.", "contents": "Cochlear implants: technical and clinical implications. Recent investigations using animal models and human subjects have helped to define the limits which are achievable in terms of information transfer with single-channel electrical stimulation of the auditory nerve. These studies are reviewed, and presented as a context for a description of the development of an implantable multiple-electrode multiplexing system which may provide control for several stimulus channels. While such a device may enable the stimulation of small segments of the auditory nerve independently, it does not contain any means of complex stimulus analysis and recording. This latter aspect of prosthesis development remains as the most formidable problem, from a technical viewpoint, that stands in the way of any significant progress.", "PMID": 957846}
{"id": "pubmed23n0032_2905", "title": "Apnea and airway obstruction during feeding and sleep.", "content": "Recent awareness of the magnitude of sudden unexplained deaths in apparently healthy infants has lead to an increased interest in those circumstances that are associated with or can elicit prolonged and serious apneic episodes. In the present studies, attention was directed toward the study of physiologic activity during sleep and feeding. Apneic episodes of varying durations occur during sleep which, in some instances, can be of sufficient length to warrant resuscitative intervention. A number of infants also reveal transient upper airway obstruction following brief periods of sleep apnea. This functional airway obstruction produces sudden and severe bradycardia. Similarly, infant feeding can induce dangerously prolonged periods of apnea and, in some infants, transient airway obstruction. Few detailed studies have been performed to identify the anatomical level or characteristics of the obstruction. Available evidence suggests that this can take the form either of muscle hypotonicity or hypertonicity. Two infants observed by means of direct laryngoscopy revealed transient failure of vocal cord abduction. These results have provided for the development of two theoretical models that can result in the sudden infant death syndrome; furthermore, continuous recordings of respiratory and cardiac activity during feeding and sleep can be extremely valuable in elucidating the mechanisms responsible for the sudden development of apneic and cyanotic episodes in infants.", "contents": "Apnea and airway obstruction during feeding and sleep. Recent awareness of the magnitude of sudden unexplained deaths in apparently healthy infants has lead to an increased interest in those circumstances that are associated with or can elicit prolonged and serious apneic episodes. In the present studies, attention was directed toward the study of physiologic activity during sleep and feeding. Apneic episodes of varying durations occur during sleep which, in some instances, can be of sufficient length to warrant resuscitative intervention. A number of infants also reveal transient upper airway obstruction following brief periods of sleep apnea. This functional airway obstruction produces sudden and severe bradycardia. Similarly, infant feeding can induce dangerously prolonged periods of apnea and, in some infants, transient airway obstruction. Few detailed studies have been performed to identify the anatomical level or characteristics of the obstruction. Available evidence suggests that this can take the form either of muscle hypotonicity or hypertonicity. Two infants observed by means of direct laryngoscopy revealed transient failure of vocal cord abduction. These results have provided for the development of two theoretical models that can result in the sudden infant death syndrome; furthermore, continuous recordings of respiratory and cardiac activity during feeding and sleep can be extremely valuable in elucidating the mechanisms responsible for the sudden development of apneic and cyanotic episodes in infants.", "PMID": 957847}
{"id": "pubmed23n0032_2906", "title": "Cardiac and pulmonary failure secondary to adenotonsillar hypertrophy.", "content": "For over 15 years, upper respiratory tract obstruction due to adenotonsillar hypertrophy has been known to cause hypoxia, hypercapnia, increased pulmonary vascular resistance and thereby cor pulmonale and congestive heart failure. This is now an uncommon but not rare entity and three recent cases prompted this report. The typical patient is dyspneic with retractions, cyanosis, occasional periods of apnea and somnolence. Edema and hepatomegaly and at times splenomegaly are common. X-rays show cardiomegaly, which on electrocardiogram is found to involved mainly the right ventricle. The strict definition of cor pulmonale is right ventricular hypertrophy secondary to lung disease or abnormal pulmonary function, a definition that may logically be stretched to include abnormal respiratory function secondary to upper airway pathology. The mechanisms by which this occurs are generally agreed upon. Hypoxia has been demonstrated to cause pulmonary vasoconstriction. Acidosis and hypercapnia are thought by some to have the same effect. Pressure across the pulmonary vascular bed is also increased, as predicted by Poiseuille's law, by the high rate of blood flow required to maintain tissue oxygenation with poorly oxygenated blood. Conditions producing hypoxia of hypercapnia or both lead to hypertrophy and eventually to dilatation of the right ventricle. Three cases of children who underwent cardiac catheterization while suffering from cor pulmonale due to adenotonsillar hypertrophy are reported. Right ventricular pressure averaged 44/5, PAO2 72, pH 7.32, and PACO2 52. All were clinically improved following adenotonsillectomy. Cardiac catheterization was repeated in one case, with right ventricular pressure dropping from 44/5 to 21/2, pulmonary vascular resistance from eight units to three, and PACO2 from 62 to 44.", "contents": "Cardiac and pulmonary failure secondary to adenotonsillar hypertrophy. For over 15 years, upper respiratory tract obstruction due to adenotonsillar hypertrophy has been known to cause hypoxia, hypercapnia, increased pulmonary vascular resistance and thereby cor pulmonale and congestive heart failure. This is now an uncommon but not rare entity and three recent cases prompted this report. The typical patient is dyspneic with retractions, cyanosis, occasional periods of apnea and somnolence. Edema and hepatomegaly and at times splenomegaly are common. X-rays show cardiomegaly, which on electrocardiogram is found to involved mainly the right ventricle. The strict definition of cor pulmonale is right ventricular hypertrophy secondary to lung disease or abnormal pulmonary function, a definition that may logically be stretched to include abnormal respiratory function secondary to upper airway pathology. The mechanisms by which this occurs are generally agreed upon. Hypoxia has been demonstrated to cause pulmonary vasoconstriction. Acidosis and hypercapnia are thought by some to have the same effect. Pressure across the pulmonary vascular bed is also increased, as predicted by Poiseuille's law, by the high rate of blood flow required to maintain tissue oxygenation with poorly oxygenated blood. Conditions producing hypoxia of hypercapnia or both lead to hypertrophy and eventually to dilatation of the right ventricle. Three cases of children who underwent cardiac catheterization while suffering from cor pulmonale due to adenotonsillar hypertrophy are reported. Right ventricular pressure averaged 44/5, PAO2 72, pH 7.32, and PACO2 52. All were clinically improved following adenotonsillectomy. Cardiac catheterization was repeated in one case, with right ventricular pressure dropping from 44/5 to 21/2, pulmonary vascular resistance from eight units to three, and PACO2 from 62 to 44.", "PMID": 957848}
{"id": "pubmed23n0032_2907", "title": "Medullary carcinoma of the thyroid gland.", "content": "Endocrine as well as oncologic attributes mark this rare tumor as unique. It is derived from \"C cells\" resembling the parafollicular cells of lower animals; these cells elaborate Calcitonin which acts as a sensitive signal of the presence of the tumor. Other endocrinopathies are present in 70 percent of cases so that the tumor may present as part of a complex but increasing well known clinical picture. In 10 percent the tumor occurs as an autosomal dominant trait; in the remainder, the occurrence is sporadic. As expected the lesions are \"cold\" on I131 scan. The histologic appearance of amyloid is characteristic. Regional lymph node metastasis occurs early. The tumor deserves appropriate aggressive management. Essentially surgical therapy should begin early and vigorously; the minimum procedure should be total thyroidectomy. Frequent lymph node metastasis speaks for the need for radical neck dissection which should be extended into the superior mediastinum. Management should also include the frequently associated endocrinopathies, particularly pheochromocytomas which must be suspected and eradicted prior to treatment of the thyroid tumor. A genetic workup should be included. Six cases are described, ranging in age from the third to the eighth decade. Only two were free of disease at this writing, two were alive with their disease and two were dead of their disease. All were of the sporadic group, and only one had associated endocrinopathies. None of these cases were diagnosed preoperatively.", "contents": "Medullary carcinoma of the thyroid gland. Endocrine as well as oncologic attributes mark this rare tumor as unique. It is derived from \"C cells\" resembling the parafollicular cells of lower animals; these cells elaborate Calcitonin which acts as a sensitive signal of the presence of the tumor. Other endocrinopathies are present in 70 percent of cases so that the tumor may present as part of a complex but increasing well known clinical picture. In 10 percent the tumor occurs as an autosomal dominant trait; in the remainder, the occurrence is sporadic. As expected the lesions are \"cold\" on I131 scan. The histologic appearance of amyloid is characteristic. Regional lymph node metastasis occurs early. The tumor deserves appropriate aggressive management. Essentially surgical therapy should begin early and vigorously; the minimum procedure should be total thyroidectomy. Frequent lymph node metastasis speaks for the need for radical neck dissection which should be extended into the superior mediastinum. Management should also include the frequently associated endocrinopathies, particularly pheochromocytomas which must be suspected and eradicted prior to treatment of the thyroid tumor. A genetic workup should be included. Six cases are described, ranging in age from the third to the eighth decade. Only two were free of disease at this writing, two were alive with their disease and two were dead of their disease. All were of the sporadic group, and only one had associated endocrinopathies. None of these cases were diagnosed preoperatively.", "PMID": 957849}
{"id": "pubmed23n0032_2908", "title": "Acute acromegalic dyspnea.", "content": "A case of acute \"acromegalic dyspnea\" due to vocal cord fixation and subglottic mucosal hypertrophy is reported. The patient was treated by intubation, tracheostomy, stereotactic implantation of radioisotopes 192Ir and 198Au via the transsphenoidal route.", "contents": "Acute acromegalic dyspnea. A case of acute \"acromegalic dyspnea\" due to vocal cord fixation and subglottic mucosal hypertrophy is reported. The patient was treated by intubation, tracheostomy, stereotactic implantation of radioisotopes 192Ir and 198Au via the transsphenoidal route.", "PMID": 957851}
{"id": "pubmed23n0032_2909", "title": "Pseudomonas meningitis complicating head and neck surgery.", "content": "Three patients, each of whom had Pseudomonas meningitis as a sequela of an extensive head and neck operation, have been treated successfully. All three patients had cerebrospinal fluid leaks, and operative management of this complication is discussed. Antibiotic management included the parenteral administration of the recently developed drugs gentamicin, carbenicillin, and intrathecal gentamicin. Since extensive head and neck operations are being performed, with increasing frequency and since infectious complications are inevitable, it is mandatory that the otolaryngologist be familiar with current methods of managing these potentially lethal conditions.", "contents": "Pseudomonas meningitis complicating head and neck surgery. Three patients, each of whom had Pseudomonas meningitis as a sequela of an extensive head and neck operation, have been treated successfully. All three patients had cerebrospinal fluid leaks, and operative management of this complication is discussed. Antibiotic management included the parenteral administration of the recently developed drugs gentamicin, carbenicillin, and intrathecal gentamicin. Since extensive head and neck operations are being performed, with increasing frequency and since infectious complications are inevitable, it is mandatory that the otolaryngologist be familiar with current methods of managing these potentially lethal conditions.", "PMID": 957850}
{"id": "pubmed23n0032_2910", "title": "Chondrodermatitis helicis: a clinical re-evaluation and pathological review.", "content": "Chondrodermatitis helicis, or painful nodule of the ear, is an uncommon benign aural lesion which is seen and treated by dermatologists and otolaryngologists. Because of the sparcity of reports in the recent literature, our experience with 50 patients over a 10-year period is presented. The diagnosis is based on history and physical examination and biopsy need be performed only to confirm the diagnosis in atypical cases. Patients are generally middle-aged or elderly males. There are no associated systemic disorders with this condition. The lesions are discrete, grey to red in color, oval shaped with raised rolled edges, and a central ulcer or depression which often contains a crust or scale. The lesion is typically painful and tender and, for this reason, the patient seeks help shortly after the onset of symptoms. The characteristic histopathologic features are epithelial hyperplasia, collagen degeneration, focal fibrinoid necrosis, and inflammatory components. Clinically, the lesion is misdiagnosed in the majority of instances and is presumably, therefore, treated inappropriately. It should be stressed that this is a benign condition and initial management should be aimed at conservative therapy with local steroid injection or conservative non-deforming surgery. Wide excision, including removal of the underlying cartilage with appropriate reconstrictive closure should be reserved for the conservative treatment failures.", "contents": "Chondrodermatitis helicis: a clinical re-evaluation and pathological review. Chondrodermatitis helicis, or painful nodule of the ear, is an uncommon benign aural lesion which is seen and treated by dermatologists and otolaryngologists. Because of the sparcity of reports in the recent literature, our experience with 50 patients over a 10-year period is presented. The diagnosis is based on history and physical examination and biopsy need be performed only to confirm the diagnosis in atypical cases. Patients are generally middle-aged or elderly males. There are no associated systemic disorders with this condition. The lesions are discrete, grey to red in color, oval shaped with raised rolled edges, and a central ulcer or depression which often contains a crust or scale. The lesion is typically painful and tender and, for this reason, the patient seeks help shortly after the onset of symptoms. The characteristic histopathologic features are epithelial hyperplasia, collagen degeneration, focal fibrinoid necrosis, and inflammatory components. Clinically, the lesion is misdiagnosed in the majority of instances and is presumably, therefore, treated inappropriately. It should be stressed that this is a benign condition and initial management should be aimed at conservative therapy with local steroid injection or conservative non-deforming surgery. Wide excision, including removal of the underlying cartilage with appropriate reconstrictive closure should be reserved for the conservative treatment failures.", "PMID": 957852}
{"id": "pubmed23n0032_2911", "title": "The role of cellular degeneration in the normal development of (rat) otocyst.", "content": "From day 12 to 16 of gestation a highly circumscribed zone of cellular degeneration has been observed in the otocyst of more than 50 normal rats. This zone appears to be produced by selective or programmed cell death, and results in the elimination of unneeded cellular elements. That cells in this zone are also selectively destroying subcellular organelles as a first step in specialization is also discussed. Programmed cellular death has long been known in other systems and is often an obligatory companion to cellular specialization. The ear now joins the long list of organs which demonstrate this phenomenon.", "contents": "The role of cellular degeneration in the normal development of (rat) otocyst. From day 12 to 16 of gestation a highly circumscribed zone of cellular degeneration has been observed in the otocyst of more than 50 normal rats. This zone appears to be produced by selective or programmed cell death, and results in the elimination of unneeded cellular elements. That cells in this zone are also selectively destroying subcellular organelles as a first step in specialization is also discussed. Programmed cellular death has long been known in other systems and is often an obligatory companion to cellular specialization. The ear now joins the long list of organs which demonstrate this phenomenon.", "PMID": 957853}
{"id": "pubmed23n0032_2912", "title": "An experimental model for the study of endolymphatic hydrops in sharks: implications for the clinician.", "content": "In recent years, attention has focused on the role of the endolymphatic sac (ELS) and the endolymphatic duct (ELD) in the pathogenesis of endolymphatic hydrops (ELH). Changes have been noted surgically and radiographically by others in the ELS and ELD in patients with ELH. This report summarizes the development of a shark model with which to study the pathophysiology of ELH. The background material and overall results of anatomic, histopathologic, and ultrastructural studies using the model are presented. Possible implications for the clinical handling of ELH as a result of this work is emphasized.", "contents": "An experimental model for the study of endolymphatic hydrops in sharks: implications for the clinician. In recent years, attention has focused on the role of the endolymphatic sac (ELS) and the endolymphatic duct (ELD) in the pathogenesis of endolymphatic hydrops (ELH). Changes have been noted surgically and radiographically by others in the ELS and ELD in patients with ELH. This report summarizes the development of a shark model with which to study the pathophysiology of ELH. The background material and overall results of anatomic, histopathologic, and ultrastructural studies using the model are presented. Possible implications for the clinical handling of ELH as a result of this work is emphasized.", "PMID": 957854}
{"id": "pubmed23n0032_2913", "title": "Alkaloids of Carnegiea gigantea. Arizonine, a new tetrahydroisoquinoline alkaloid.", "content": "The alkaloid composition of the giant cactus, Carnegiea gigantea Br. & R., was studied. Chromatographic separation led to the isolation of four tetrahydroisoquinoline alkaloids: carnegine, salsolidine, gigantine and arizonine. The structure of the new natural product, arizonine, was establihsed by spectroscopic data and total synthesis via two independent routes. Salsolidine is reported for the first time in the cacti. Also new to this species are 3-methoxytyramine and 3,4-dimethoxypenethylamine, which were identified in the alkaloid extract by gas chromatography-mass spectrometry. Dopamine was isolated from fresh plant material.", "contents": "Alkaloids of Carnegiea gigantea. Arizonine, a new tetrahydroisoquinoline alkaloid. The alkaloid composition of the giant cactus, Carnegiea gigantea Br. & R., was studied. Chromatographic separation led to the isolation of four tetrahydroisoquinoline alkaloids: carnegine, salsolidine, gigantine and arizonine. The structure of the new natural product, arizonine, was establihsed by spectroscopic data and total synthesis via two independent routes. Salsolidine is reported for the first time in the cacti. Also new to this species are 3-methoxytyramine and 3,4-dimethoxypenethylamine, which were identified in the alkaloid extract by gas chromatography-mass spectrometry. Dopamine was isolated from fresh plant material.", "PMID": 957908}
{"id": "pubmed23n0032_2914", "title": "DIOXINS/*ISOLof West African medicinal plants. XV. Dinklacorine, a new biphenyl dibenzodioxin alkaloid from Tiliacora dinklagei.", "content": "Dinklacorine was first isolated in 1974 from extracts of the roots of Tiliacora dinklagei Engl. (Menispermaceae) and designated TD-2. The ir, uv and mass spectra of dinklacorine were very similar to those of the dibenzodioxin biphenylalkaloid tiliacorine. However, the two alkaloids differed in their mp, specific rotation and nmr spectra. Methylation of dinklacorine with diazomethane afforded O-methyltiliacorine while treatment of dinklacorine with sodium methoxide and methyl iodide gave O-methyltiliacorine dimethiodide. However, prolonged treatment of tiliacorine and dinklacorine with diazoethane afforded different O-ethyl ethers. In addition, O-ethyldinklacorine dimethiodide and O-ethyltiliacorne dimethiodide were different. Furthermore, acetylation of the two alkaloids with acetic anhydride and puridine gave O-macetyl esters which were not identical. A considertation to these data and especially the mass spectral fragmentation patterns indicated dinklacorine to be a positional isomer of tiliacorine with the phenolic hydroxy group present in the biphenyl portion of the molecule on the opposite side to tiliacorine. Finally, a comparison of the cd spectra of dinklacorine and tiliacorine suggests that they have the same stereochemistry.", "contents": "DIOXINS/*ISOLof West African medicinal plants. XV. Dinklacorine, a new biphenyl dibenzodioxin alkaloid from Tiliacora dinklagei. Dinklacorine was first isolated in 1974 from extracts of the roots of Tiliacora dinklagei Engl. (Menispermaceae) and designated TD-2. The ir, uv and mass spectra of dinklacorine were very similar to those of the dibenzodioxin biphenylalkaloid tiliacorine. However, the two alkaloids differed in their mp, specific rotation and nmr spectra. Methylation of dinklacorine with diazomethane afforded O-methyltiliacorine while treatment of dinklacorine with sodium methoxide and methyl iodide gave O-methyltiliacorine dimethiodide. However, prolonged treatment of tiliacorine and dinklacorine with diazoethane afforded different O-ethyl ethers. In addition, O-ethyldinklacorine dimethiodide and O-ethyltiliacorne dimethiodide were different. Furthermore, acetylation of the two alkaloids with acetic anhydride and puridine gave O-macetyl esters which were not identical. A considertation to these data and especially the mass spectral fragmentation patterns indicated dinklacorine to be a positional isomer of tiliacorine with the phenolic hydroxy group present in the biphenyl portion of the molecule on the opposite side to tiliacorine. Finally, a comparison of the cd spectra of dinklacorine and tiliacorine suggests that they have the same stereochemistry.", "PMID": 957910}
{"id": "pubmed23n0032_2915", "title": "Alkaloids of Thalictrum. XV. Isolation and identification of the hypotensive alkaloids of the root of Thalictrum lucidum.", "content": "Sixteen alkaloids, namely homoaromoline, obaberine, O-methyl-thalicberine, oxyberberine, thalidasine, thaliglucinone, thalrugosine, obamegine, oxyacanthine, berberine, columbamine, jatrorrhizine, magnoflorine, palmatine, thalifendine and base A chloride, plus the artifact, 8-trichloromethyldihydroberberine, were isolated from the alkaloid fraction of the roots of Thalictrum lucidum L. Of these, obamegine, thalrugosine, O-methylthalicberine, thaliglucinone, obaberine and homoaromoline were found to possess hypotensive activity in normotensive dogs. Thalidasine, homoaromoline, thalrugosine, thaliglucinone, obamegine, jatrorrhizine, and columbamine were found to possess antimicrobial activity against Mycobacterium smegmatis at a concentration of 100 mug/ml or less.", "contents": "Alkaloids of Thalictrum. XV. Isolation and identification of the hypotensive alkaloids of the root of Thalictrum lucidum. Sixteen alkaloids, namely homoaromoline, obaberine, O-methyl-thalicberine, oxyberberine, thalidasine, thaliglucinone, thalrugosine, obamegine, oxyacanthine, berberine, columbamine, jatrorrhizine, magnoflorine, palmatine, thalifendine and base A chloride, plus the artifact, 8-trichloromethyldihydroberberine, were isolated from the alkaloid fraction of the roots of Thalictrum lucidum L. Of these, obamegine, thalrugosine, O-methylthalicberine, thaliglucinone, obaberine and homoaromoline were found to possess hypotensive activity in normotensive dogs. Thalidasine, homoaromoline, thalrugosine, thaliglucinone, obamegine, jatrorrhizine, and columbamine were found to possess antimicrobial activity against Mycobacterium smegmatis at a concentration of 100 mug/ml or less.", "PMID": 957909}
{"id": "pubmed23n0032_2916", "title": "Tumor inhibitors. 114. Aloe emodin: antileukemic principle isolated from Rhamnus frangula L.", "content": "A systematic fractionation of an ethanol-water (1:1) extract of the seeds of Rhamnus frangula L., guided by assays for tumore-inhibitory activity, led to the isolation of aloe emodin (1). This compound was found to show significant antileukemic activity against the P-388 lymphocytic leukemia in mice. A note-worthy vehicle-dependence of the testing results is reported. In the light of this vehicle-dependence, the re-examination of other anthraquinone derivatives is recommended.", "contents": "Tumor inhibitors. 114. Aloe emodin: antileukemic principle isolated from Rhamnus frangula L. A systematic fractionation of an ethanol-water (1:1) extract of the seeds of Rhamnus frangula L., guided by assays for tumore-inhibitory activity, led to the isolation of aloe emodin (1). This compound was found to show significant antileukemic activity against the P-388 lymphocytic leukemia in mice. A note-worthy vehicle-dependence of the testing results is reported. In the light of this vehicle-dependence, the re-examination of other anthraquinone derivatives is recommended.", "PMID": 957911}
{"id": "pubmed23n0032_2917", "title": "Automated short range locator system.", "content": "A computer operated system is described for determining the location of a subject fitted with a small sensing coil and telemetry transmitter. The system uses the geometric factors inherent in the value of the mutual inductance of a fixed and moveable coil. Results of a two-dimensional system are shown.", "contents": "Automated short range locator system. A computer operated system is described for determining the location of a subject fitted with a small sensing coil and telemetry transmitter. The system uses the geometric factors inherent in the value of the mutual inductance of a fixed and moveable coil. Results of a two-dimensional system are shown.", "PMID": 957921}
{"id": "pubmed23n0032_2918", "title": "Screening of Chilean plants for anticancer activity. I.", "content": "Extracts prepared from 519 samples of plant material were evaluated for anticancer activity against the usual systems. The results of the antitumor testing showed that 156 of these extracts gave initial indications of anticancer activity in one or more tumor systems. The activity was confirmed in 14 samples.", "contents": "Screening of Chilean plants for anticancer activity. I. Extracts prepared from 519 samples of plant material were evaluated for anticancer activity against the usual systems. The results of the antitumor testing showed that 156 of these extracts gave initial indications of anticancer activity in one or more tumor systems. The activity was confirmed in 14 samples.", "PMID": 957912}
{"id": "pubmed23n0032_2919", "title": "An electro-acoustical technique for the detection of knee joint noise.", "content": "Distinguishing acoustical signatures of sound emitted by normal and pathological knee joints are picked up using a double microphone-differential amplifier setup. Extraneous background noise is minimized using the principle of \"noise cancellation\". Two identical sensitive condenser microphones and an F.M. recorder with flat responses in the audio range were used. Preliminary studies covering normal and diseased knee joints showed that their respective waveforms and spectral patterns are unique and proved to be a promising nondestructive diagnostic tool for early detection of knee joint cartilage damage.", "contents": "An electro-acoustical technique for the detection of knee joint noise. Distinguishing acoustical signatures of sound emitted by normal and pathological knee joints are picked up using a double microphone-differential amplifier setup. Extraneous background noise is minimized using the principle of \"noise cancellation\". Two identical sensitive condenser microphones and an F.M. recorder with flat responses in the audio range were used. Preliminary studies covering normal and diseased knee joints showed that their respective waveforms and spectral patterns are unique and proved to be a promising nondestructive diagnostic tool for early detection of knee joint cartilage damage.", "PMID": 957922}
{"id": "pubmed23n0032_2920", "title": "The effect of external pressure on skin temperature distribution by thermography.", "content": "1) Serial thermograms were taken after the application of various intensities and durations of localized pressure on the medial surface of the human forearm. 2) Thermal response of the body surface to localized pressure was quantified. 3) It was found that usually a time period of between 1 to 3 minutes elapsed before the body surface attained a maximum elevation of temperature from the time the localized pressure is removed. 4) It was found that increased intensity of pressure (5 psi vs. 3 psi) and longer duration (20 minutes vs. 10 minutes) caused larger thermal responses. 5) It is suggested that high resolution medical thermography may eventually become a useful tool in the fitting of prosthetic and orthotic devices and for the prediction of pressure sores formation so prophylactic measures can be started immediately on immobilized patients.", "contents": "The effect of external pressure on skin temperature distribution by thermography. 1) Serial thermograms were taken after the application of various intensities and durations of localized pressure on the medial surface of the human forearm. 2) Thermal response of the body surface to localized pressure was quantified. 3) It was found that usually a time period of between 1 to 3 minutes elapsed before the body surface attained a maximum elevation of temperature from the time the localized pressure is removed. 4) It was found that increased intensity of pressure (5 psi vs. 3 psi) and longer duration (20 minutes vs. 10 minutes) caused larger thermal responses. 5) It is suggested that high resolution medical thermography may eventually become a useful tool in the fitting of prosthetic and orthotic devices and for the prediction of pressure sores formation so prophylactic measures can be started immediately on immobilized patients.", "PMID": 957924}
{"id": "pubmed23n0032_2921", "title": "A new biomedical mechanical impedance bridge.", "content": "A system is described which measures directly the driving-point force, velocity and complex mechanical impedance of biological, non-biological and viscoelastic materials and structures. The system uses a single transducer and is capable of measuring the velocity responses to sinusoidal, linear, step and other forcing functions.", "contents": "A new biomedical mechanical impedance bridge. A system is described which measures directly the driving-point force, velocity and complex mechanical impedance of biological, non-biological and viscoelastic materials and structures. The system uses a single transducer and is capable of measuring the velocity responses to sinusoidal, linear, step and other forcing functions.", "PMID": 957925}
{"id": "pubmed23n0032_2922", "title": "Iowa wrestling study: weight classification systems.", "content": "Many wrestlers lose a large amount of weight in a relatively short period of time to become certified for interscholastic competition. In most states, there are twelve certified weight classes with intervals ranging from 6 to 18 pounds. Using anthropometric data obtained from 834 \"average\" wrestlers that were measured 5-8 weeks before competition, we predicted their \"minimal\" body weights and assigned them into arbitrarily designated weight classes of 6, 8 and 10 pound intervals. In addition, the 1973-74 and the 1974-75 certifying records for Iowa wrestlers were examined with regard to frequency distribution patterns. The results indicated that the majority of students were certifying for a weight classes. In fast, forty percent of the more than 8900 students were certified for the 119-138 classes and 57% of the population were located within the 112-145 weight classes. Inspection of the frequency distribution patterns of wrestlers assigned to the arbitrary classes also showed a marked concentration of wrestlers who weighed between 120 and 140 pounds and this relationship became more apparent when ten pound intervals were considered. From these findings we recommended that state high school associations consider: 1) allowing more than one wrestler per team to compete in a single weight class; 2) porviding more weight classes than the current twelve; and 3) promoting body composition assessments during the pre-season period in order to minimize the problems created by the \"making of weight.\"", "contents": "Iowa wrestling study: weight classification systems. Many wrestlers lose a large amount of weight in a relatively short period of time to become certified for interscholastic competition. In most states, there are twelve certified weight classes with intervals ranging from 6 to 18 pounds. Using anthropometric data obtained from 834 \"average\" wrestlers that were measured 5-8 weeks before competition, we predicted their \"minimal\" body weights and assigned them into arbitrarily designated weight classes of 6, 8 and 10 pound intervals. In addition, the 1973-74 and the 1974-75 certifying records for Iowa wrestlers were examined with regard to frequency distribution patterns. The results indicated that the majority of students were certifying for a weight classes. In fast, forty percent of the more than 8900 students were certified for the 119-138 classes and 57% of the population were located within the 112-145 weight classes. Inspection of the frequency distribution patterns of wrestlers assigned to the arbitrary classes also showed a marked concentration of wrestlers who weighed between 120 and 140 pounds and this relationship became more apparent when ten pound intervals were considered. From these findings we recommended that state high school associations consider: 1) allowing more than one wrestler per team to compete in a single weight class; 2) porviding more weight classes than the current twelve; and 3) promoting body composition assessments during the pre-season period in order to minimize the problems created by the \"making of weight.\"", "PMID": 957927}
{"id": "pubmed23n0032_2923", "title": "Iowa wrestling study: weight loss and urinary profiles of collegiate wrestlers.", "content": "A longitudinal study was conducted with various members of the 1975 University of Iowa NCAA championship wrestling team to determine if excessive weight loss, accompanied by signs of dehydration, occurred at the college level of competition. Body weight changes from September to December indicated a mean loss of 6%, while skinfold totals (6 sites) changed from a mean of 58 mm to 37 mm. During a four-month period, mean weight losses of 10.2, 9.5, 8.0, 7.5 and 7.0 lbs occurred in intervals of 12, 4, 3, 2 and 1 day, respectively. Basal urines analyzed throughout the season usually contained 2-3 times the potassium excreted before the season started. Analyses of urines at various intervals during a 2-day time period prior to weigh-in showed a .003 increase in specific gravity, 160 mosm/1 increase in osmolarity, .10 decrease in pH, 45.3 mEq/1 decrease in Na+ concentration, and a 71.3 mEq/1 increase in K+ concentration which suggested that the wrestlers were dehydrated prior to competition. Total urinary electrolyte loss during the 2 days amounted to 3.7% of estimated total body Na+ stores and 3.0% of total body K+ stores. These data were similar to what had been reported for Iowa high school finalists and indicated that collegiate wrestlers were also competing while in a dehydrated state.", "contents": "Iowa wrestling study: weight loss and urinary profiles of collegiate wrestlers. A longitudinal study was conducted with various members of the 1975 University of Iowa NCAA championship wrestling team to determine if excessive weight loss, accompanied by signs of dehydration, occurred at the college level of competition. Body weight changes from September to December indicated a mean loss of 6%, while skinfold totals (6 sites) changed from a mean of 58 mm to 37 mm. During a four-month period, mean weight losses of 10.2, 9.5, 8.0, 7.5 and 7.0 lbs occurred in intervals of 12, 4, 3, 2 and 1 day, respectively. Basal urines analyzed throughout the season usually contained 2-3 times the potassium excreted before the season started. Analyses of urines at various intervals during a 2-day time period prior to weigh-in showed a .003 increase in specific gravity, 160 mosm/1 increase in osmolarity, .10 decrease in pH, 45.3 mEq/1 decrease in Na+ concentration, and a 71.3 mEq/1 increase in K+ concentration which suggested that the wrestlers were dehydrated prior to competition. Total urinary electrolyte loss during the 2 days amounted to 3.7% of estimated total body Na+ stores and 3.0% of total body K+ stores. These data were similar to what had been reported for Iowa high school finalists and indicated that collegiate wrestlers were also competing while in a dehydrated state.", "PMID": 957928}
{"id": "pubmed23n0032_2924", "title": "Weight and skinfold thickness changes during a physical training course.", "content": "The effect of an intensive physical training program on body weight and skinfold thickness was compared in subjects who had initially small (group S), medium (group M) and large (group L) skinfold thickness representing mostly thin, normal and obese recruits, respectively. Weight and skinfold thickness at three sites (triceps, subscapula and abdomen) were measured at the beginning and end of a 6 week intensive military training program. Subjects were on an ad-lib diet. Seventy-one percent of the subjects in group S increased their body weight and 60% increased the sum of their skinfold thickness (SSFT) at the three sites during the training period. These increases were significant at P less than 0.001 and P less than 0.01 for weight and SSFT respectively. In groups M and L the trends for changes in SSFT were in the opposite direction, with 68% of the former and 98% of the latter showing decreases in SSFT. The mean decreases were significant at P less than 0.02 and P less than 0.001 for groups M and L respectively. While the subjects in group L lost weight significantly (P less than 0.001), those in group M reduced their weight only slightly. Our data indicate that changes in body fat, indicated by changes in SSFT, during several weeks of physical activity are not unidirectional and are related to the initial skinfold thickness.", "contents": "Weight and skinfold thickness changes during a physical training course. The effect of an intensive physical training program on body weight and skinfold thickness was compared in subjects who had initially small (group S), medium (group M) and large (group L) skinfold thickness representing mostly thin, normal and obese recruits, respectively. Weight and skinfold thickness at three sites (triceps, subscapula and abdomen) were measured at the beginning and end of a 6 week intensive military training program. Subjects were on an ad-lib diet. Seventy-one percent of the subjects in group S increased their body weight and 60% increased the sum of their skinfold thickness (SSFT) at the three sites during the training period. These increases were significant at P less than 0.001 and P less than 0.01 for weight and SSFT respectively. In groups M and L the trends for changes in SSFT were in the opposite direction, with 68% of the former and 98% of the latter showing decreases in SSFT. The mean decreases were significant at P less than 0.02 and P less than 0.001 for groups M and L respectively. While the subjects in group L lost weight significantly (P less than 0.001), those in group M reduced their weight only slightly. Our data indicate that changes in body fat, indicated by changes in SSFT, during several weeks of physical activity are not unidirectional and are related to the initial skinfold thickness.", "PMID": 957929}
{"id": "pubmed23n0032_2925", "title": "Effect of physical fitness on the adrenocortical response to exercise stress.", "content": "Serum corticosteroid (S.C.) responses were compared in two groups of middle-aged male subjects, one sedentary, one active (both N = 11, mean age 44 years), who performed graded bicycle ergometer exercise before (pre) and after (post) a 4 month physical fitness program. Pre-training exercise involved low and high work intensities which required similar percentage of predicted Vo2 max for the sedentary and active groups. Post-training exercise at identical work loads which resulted in lower work intensities due to training effects, was followed by increased work loads demanding higher work intensities in order to accommodate for the increased work capacity of the respective groups. The sedentary group (pre) and the active group (post) showed significant elevations in S.C. during high intensity exercise and recovery. The active group had significantly lower S.C. during low and high intensity exercise and recovery (pre), and during rest and low intensity exercise (post). The sedentary group had significantly lower S.C. at identical work loads which resulted in a reduction of relative work intensity from pre to post-traing tests. Results suggest both fitness status and training effects in the adrenocortical response to exercise stress, although psychological factors may be involved.", "contents": "Effect of physical fitness on the adrenocortical response to exercise stress. Serum corticosteroid (S.C.) responses were compared in two groups of middle-aged male subjects, one sedentary, one active (both N = 11, mean age 44 years), who performed graded bicycle ergometer exercise before (pre) and after (post) a 4 month physical fitness program. Pre-training exercise involved low and high work intensities which required similar percentage of predicted Vo2 max for the sedentary and active groups. Post-training exercise at identical work loads which resulted in lower work intensities due to training effects, was followed by increased work loads demanding higher work intensities in order to accommodate for the increased work capacity of the respective groups. The sedentary group (pre) and the active group (post) showed significant elevations in S.C. during high intensity exercise and recovery. The active group had significantly lower S.C. during low and high intensity exercise and recovery (pre), and during rest and low intensity exercise (post). The sedentary group had significantly lower S.C. at identical work loads which resulted in a reduction of relative work intensity from pre to post-traing tests. Results suggest both fitness status and training effects in the adrenocortical response to exercise stress, although psychological factors may be involved.", "PMID": 957930}
{"id": "pubmed23n0032_2926", "title": "The effect of saddle height on oxygen consumption during bicycle ergometer work.", "content": "The effect of saddle height upon oxygen consumption during bicycle ergometer work was measured. Five subjects were tested on a continuous work protocol from 50 watts to 200 watts in 25 watt increments at experimental saddle heights of 100%, 103%, 106%, 109%, and 112% of inside leg length measured from the ischium to the floor. Data were recorded on Vo2, Vco2, VE, and heart rate. It was found that Vo2 progressively increased as saddle height increased; the highest Vo2 occurred at the highest experimental setting of 112%; the most effective saddle positions in the experiment as measured by lowest Vo2 per unit of work were 100% and 103%; and there was no significant difference between the VO2 AT 100% AND 103%. It was concluded that in light of our data and earlier data showing power output to be maximized at 104% (by our measurement method), the saddle height of choice should be approximately 103% to 104% of leg length. A convenient and objective method for setting seat height is presented.", "contents": "The effect of saddle height on oxygen consumption during bicycle ergometer work. The effect of saddle height upon oxygen consumption during bicycle ergometer work was measured. Five subjects were tested on a continuous work protocol from 50 watts to 200 watts in 25 watt increments at experimental saddle heights of 100%, 103%, 106%, 109%, and 112% of inside leg length measured from the ischium to the floor. Data were recorded on Vo2, Vco2, VE, and heart rate. It was found that Vo2 progressively increased as saddle height increased; the highest Vo2 occurred at the highest experimental setting of 112%; the most effective saddle positions in the experiment as measured by lowest Vo2 per unit of work were 100% and 103%; and there was no significant difference between the VO2 AT 100% AND 103%. It was concluded that in light of our data and earlier data showing power output to be maximized at 104% (by our measurement method), the saddle height of choice should be approximately 103% to 104% of leg length. A convenient and objective method for setting seat height is presented.", "PMID": 957931}
{"id": "pubmed23n0032_2927", "title": "Trend analysis of the % VO2 max-HR regression.", "content": "The purpose of the study was to mathematically model the regression of percent of maximal oxygen consumption (% VO2 max) on relative (% of max) heart rate (HR). The 26 subjects (Ss) were classified based on activity levels into high, medium, and low-fitness. Each S performed a series of treadmill walks and runs ranging from 30 to 100% of max VO2. Percent of VO2 max and relative HR were determined during each exercise bout. The data were subjected to a trend analysis utilizing multiple regression techniques. The associated Rs were: linear 0.966, quadratic 0.971, cubic 0.971, and quartic 0.977. The second and fourth order terms statistically accounted for more of the variability than their predecessors, but these differences were not of practical significance. There were no statistically significant differences among the fitness subgroup regression slopes or intercepts for any of the sets of regression equations. The bivariate equation was Y = 1.369-X-40.99 (Y = % VO2 max and X=relative HR) with a standard error of the estimate of 5.67 % VO2 max.", "contents": "Trend analysis of the % VO2 max-HR regression. The purpose of the study was to mathematically model the regression of percent of maximal oxygen consumption (% VO2 max) on relative (% of max) heart rate (HR). The 26 subjects (Ss) were classified based on activity levels into high, medium, and low-fitness. Each S performed a series of treadmill walks and runs ranging from 30 to 100% of max VO2. Percent of VO2 max and relative HR were determined during each exercise bout. The data were subjected to a trend analysis utilizing multiple regression techniques. The associated Rs were: linear 0.966, quadratic 0.971, cubic 0.971, and quartic 0.977. The second and fourth order terms statistically accounted for more of the variability than their predecessors, but these differences were not of practical significance. There were no statistically significant differences among the fitness subgroup regression slopes or intercepts for any of the sets of regression equations. The bivariate equation was Y = 1.369-X-40.99 (Y = % VO2 max and X=relative HR) with a standard error of the estimate of 5.67 % VO2 max.", "PMID": 957932}
{"id": "pubmed23n0032_2928", "title": "The validity of self-perceptions regarding physical and athletic ability.", "content": "High school males (N = 109) and junior high school males (N = 112) administered the Physical Estimation and Attraction Scales (PEAS), the Tennessee Self-Concept Scale (TSCS), and a physical fitness battery. Previous research results were replicated in the h.s. group in that fitness and self-esteem were not significantly related but Estimation (of physical ability) correlated significantly with both fitness and self-esteem (p less than .01). Similar results were obtained in the jr. h.s. sample. Additionally, the validity of the Estimation (EST) scale was extended to include significant relationships with indices of emotional adjustment other than self-esteem. EST scores were shown to be influenced to significant but not inordinate degree by certain response bias tendencies. Negatively phrased EST items were denied significantly more often than positive EST items were affirmed and were more highly related to both response bias and emotional adjustment scores. Controlling for response bias influence by partial correlation failed to extinguish the significance of EST validating relationships. It was concluded that these relationships exist independently of response style or response set.", "contents": "The validity of self-perceptions regarding physical and athletic ability. High school males (N = 109) and junior high school males (N = 112) administered the Physical Estimation and Attraction Scales (PEAS), the Tennessee Self-Concept Scale (TSCS), and a physical fitness battery. Previous research results were replicated in the h.s. group in that fitness and self-esteem were not significantly related but Estimation (of physical ability) correlated significantly with both fitness and self-esteem (p less than .01). Similar results were obtained in the jr. h.s. sample. Additionally, the validity of the Estimation (EST) scale was extended to include significant relationships with indices of emotional adjustment other than self-esteem. EST scores were shown to be influenced to significant but not inordinate degree by certain response bias tendencies. Negatively phrased EST items were denied significantly more often than positive EST items were affirmed and were more highly related to both response bias and emotional adjustment scores. Controlling for response bias influence by partial correlation failed to extinguish the significance of EST validating relationships. It was concluded that these relationships exist independently of response style or response set.", "PMID": 957933}
{"id": "pubmed23n0032_2929", "title": "Degenerative hip disease in adolescent athletes.", "content": "The effect of conducted training begun at an early age on the femoral head tilt was studied in male adolescent competitive sportsmen and physical education students. The control group consisted of army recruits. The femoral head tilt proved to be smaller in both sportsmen and physical education students when compared with the control subjects. The results suggest that sports activities started at an early age would not contribute to epiphysiolysis and through that to later development of osteoarthritis in the hip joint.", "contents": "Degenerative hip disease in adolescent athletes. The effect of conducted training begun at an early age on the femoral head tilt was studied in male adolescent competitive sportsmen and physical education students. The control group consisted of army recruits. The femoral head tilt proved to be smaller in both sportsmen and physical education students when compared with the control subjects. The results suggest that sports activities started at an early age would not contribute to epiphysiolysis and through that to later development of osteoarthritis in the hip joint.", "PMID": 957934}
{"id": "pubmed23n0032_2930", "title": "Turf-toe: a shoe-surface related football injury.", "content": "Plantar capsule-ligament sprain of the great toe metatarsophalangeal joint, herein referred to as \"turf-toe,\" is discussed with emphasis on two apparantly predisposing factors, playing surface hardness and shoe stiffness. Surface hardness studies have previously been performed by the authors on natural grass and AstroTurf. A study of football shoe flexibility is presented and the results correlated with the occurrence of turftoe. We have not encountered this entity in players wearing relatively stiff conventional seven posted football shoes or the more flexible soccer style shoe on natural grass fields. We have found it to be a not uncommon injury among players wearning the soccer style shoe on AstroTurf.", "contents": "Turf-toe: a shoe-surface related football injury. Plantar capsule-ligament sprain of the great toe metatarsophalangeal joint, herein referred to as \"turf-toe,\" is discussed with emphasis on two apparantly predisposing factors, playing surface hardness and shoe stiffness. Surface hardness studies have previously been performed by the authors on natural grass and AstroTurf. A study of football shoe flexibility is presented and the results correlated with the occurrence of turftoe. We have not encountered this entity in players wearing relatively stiff conventional seven posted football shoes or the more flexible soccer style shoe on natural grass fields. We have found it to be a not uncommon injury among players wearning the soccer style shoe on AstroTurf.", "PMID": 957935}
{"id": "pubmed23n0032_2931", "title": "A cinematographic analysis of overground and treadmill running by males and females.", "content": "Cinematography was used to biomechanically compare individually selected overground jogging and running velocities with equated treadmill jogging and running by adult males and females. All subjects were regular joggers but not competitive track or cross-country runners. No significant differences were recorded in stride length, stride rate, support time or non-support time for males or females when jogging at velocities of between 3.33 and 4.78 m/s (x = 3.70) or 3.45 and 4.80 m/s (x=3.97) respectively. However, it was demonstrated that at velocities of 4.82 - 6.2 m/s for males (x - 5.41) and 4.85 - 5.76 m/s for females (x = 5.29) significant differences did occur between overground and treadmill running. For both males and females stride length decreased, stride rate increased, and the period of non-support was also significantly less when running on a treadmill as compared to running overground.", "contents": "A cinematographic analysis of overground and treadmill running by males and females. Cinematography was used to biomechanically compare individually selected overground jogging and running velocities with equated treadmill jogging and running by adult males and females. All subjects were regular joggers but not competitive track or cross-country runners. No significant differences were recorded in stride length, stride rate, support time or non-support time for males or females when jogging at velocities of between 3.33 and 4.78 m/s (x = 3.70) or 3.45 and 4.80 m/s (x=3.97) respectively. However, it was demonstrated that at velocities of 4.82 - 6.2 m/s for males (x - 5.41) and 4.85 - 5.76 m/s for females (x = 5.29) significant differences did occur between overground and treadmill running. For both males and females stride length decreased, stride rate increased, and the period of non-support was also significantly less when running on a treadmill as compared to running overground.", "PMID": 957936}
{"id": "pubmed23n0032_2932", "title": "Forces and duration of impact, and grip tightness during the tennis stroke.", "content": "The present study is concerned with the problem of the most desirable grip tightness during and after the impact phaseeof the tennis stroke. Moreover, impact forces and durations for different ball velocities are also investigated. Three series of experiments were conducted in order to obtain information on impact forces and durations, responses of the racket to impacts for loose and tight grips, and the magnitudes of the vibrations of the racket transferred to the hand in actual strokes. The experimental results were found to be in accordance with the predictions of a mathematical model of the system racket-hand. A simpler model, which neglects bending and possible translations of the racket, turns out to be inadequate for the present purpose. It is found that a tight grip increases both the impulse imparted on the ball and hence the power of the stroke, and the vibrational shocks which must be absorbed by the hand. The contrary holds true for a loose grip. An investigation on a top-class player revealed that he uses a tight grip in all his strokes. The possibility of a connection between grip tightness during and after the impact phase and the occurrence of the well-known \"tennis elbow\" is also discussed. Avoidance of the impact recoil was found to be impossible.", "contents": "Forces and duration of impact, and grip tightness during the tennis stroke. The present study is concerned with the problem of the most desirable grip tightness during and after the impact phaseeof the tennis stroke. Moreover, impact forces and durations for different ball velocities are also investigated. Three series of experiments were conducted in order to obtain information on impact forces and durations, responses of the racket to impacts for loose and tight grips, and the magnitudes of the vibrations of the racket transferred to the hand in actual strokes. The experimental results were found to be in accordance with the predictions of a mathematical model of the system racket-hand. A simpler model, which neglects bending and possible translations of the racket, turns out to be inadequate for the present purpose. It is found that a tight grip increases both the impulse imparted on the ball and hence the power of the stroke, and the vibrational shocks which must be absorbed by the hand. The contrary holds true for a loose grip. An investigation on a top-class player revealed that he uses a tight grip in all his strokes. The possibility of a connection between grip tightness during and after the impact phase and the occurrence of the well-known \"tennis elbow\" is also discussed. Avoidance of the impact recoil was found to be impossible.", "PMID": 957937}
{"id": "pubmed23n0032_2933", "title": "Muscle fiber composition and enzyme activities of elite distance runners.", "content": "Muscle biopsies were obtained from the gastrocnemius of 14 elite distance runners, 18 middle distance runners, and 19 untrained men. The middle distance runners were all highly trained, but had significantly slower performance times than the elite runners at distances greater than 3 miles. Fiber composition and mean cross-sectional areas were determined from muscle sections incubated for histochemical activity. A portion of the specimen was used to determine succinate dehydrogenase (SDH), lactate dehydrogenase (LD/Y and phosphorylase activities. All subjects were tested for maximal oxygen uptake on a treadmill. As previously demonstrated by others, the elite runners' muscles were characterized by a high percentage (79%) of slow twitch (ST) fibers. On the average, the crosssectional area of their ST fibers was found to be 22% larger than the FT fibers (P less than 0.05). SDH activity of whole muscle homogenates from elicte and middle distance runners was 3.4- and 2.8- fold greater, respectively, than that measured in the untrained men. Since the LDH and phosphorylase activities were similar for the runners and untrained men, it appears that training for distance running has little influence on the enzymes of glycogenolysis.", "contents": "Muscle fiber composition and enzyme activities of elite distance runners. Muscle biopsies were obtained from the gastrocnemius of 14 elite distance runners, 18 middle distance runners, and 19 untrained men. The middle distance runners were all highly trained, but had significantly slower performance times than the elite runners at distances greater than 3 miles. Fiber composition and mean cross-sectional areas were determined from muscle sections incubated for histochemical activity. A portion of the specimen was used to determine succinate dehydrogenase (SDH), lactate dehydrogenase (LD/Y and phosphorylase activities. All subjects were tested for maximal oxygen uptake on a treadmill. As previously demonstrated by others, the elite runners' muscles were characterized by a high percentage (79%) of slow twitch (ST) fibers. On the average, the crosssectional area of their ST fibers was found to be 22% larger than the FT fibers (P less than 0.05). SDH activity of whole muscle homogenates from elicte and middle distance runners was 3.4- and 2.8- fold greater, respectively, than that measured in the untrained men. Since the LDH and phosphorylase activities were similar for the runners and untrained men, it appears that training for distance running has little influence on the enzymes of glycogenolysis.", "PMID": 957938}
{"id": "pubmed23n0032_2934", "title": "Covert anticoagulant ingestion: study of 25 patients and review of world literature.", "content": "Twenty-five patients with covert ingestion of oral anticoagulant drugs were studied. Most of the patients were women who were either connected with the medical profession or were previously treated with antigoagulants. The most common findings were ecchymoses, hematuria, and a markedly prolonged prothrombin time. The anticoagulant drug was identified in the plasma of all 25 patients. Most patients responded promptly to administration of vitamin K1. The most common motives were malingering and suicide. The world literature was reviewed for covert ingestion of oral anticoagulant drugs and 48 other cases were found. The correct diagnosis is important to focus the physician's attention on the psychiatric rather than the somatic aspects of the disorder.", "contents": "Covert anticoagulant ingestion: study of 25 patients and review of world literature. Twenty-five patients with covert ingestion of oral anticoagulant drugs were studied. Most of the patients were women who were either connected with the medical profession or were previously treated with antigoagulants. The most common findings were ecchymoses, hematuria, and a markedly prolonged prothrombin time. The anticoagulant drug was identified in the plasma of all 25 patients. Most patients responded promptly to administration of vitamin K1. The most common motives were malingering and suicide. The world literature was reviewed for covert ingestion of oral anticoagulant drugs and 48 other cases were found. The correct diagnosis is important to focus the physician's attention on the psychiatric rather than the somatic aspects of the disorder.", "PMID": 957998}
{"id": "pubmed23n0032_2935", "title": "The extra-intestinal complications of Crohn's disease and ulcerative colitis: a study of 700 patients.", "content": "The records of a series of 700 patients with inflammatory bowel disease, 498 with Crohn's disease and 202 with ulcerative colitis, have been analyzed to determine the relative incidence and characteristic features of their extra-intestinal manifestations. The group with Crohn's disease included 62 with colitis, 223 with ileocolitis, and 213 with regional enteritis. A consideration of the clinical patterns and an understanding of their pathophysiology suggested a subdivision into two main groups: one \"colitis related\" and one related to the pathophysiology of the small nonspecific third group. Group A, colitis related, comprises joint, skin, mouth, and eye disease. The complications might be immunologically determined, were closely associated with active inflammation, and often responded to medical or surgical treatment of the underlying bowel disease. They occurred in 36% of the entire series of patients: joints were involved in 23%, skin in 15%, and mouth and eye each in 4%. Pyoderma gangrenosum was observed most often in ulcerative colitis and erythema nodosum most often in granulomatous colitis. The incidence of Group A complications was higher in disease involving the colon (42%) than in disease restricted exclusively to the small bowel (23%). There were interrelationships among the various members of Group A, with multiple manifestations occurring in a third of affected patients. Group B, related to small bowel pathophysiology, includes malabsorption, gallstones, kidney stones, and non-calculous hydronephrosis and hydroureter. Disorders in this group were generally related to the severity of the disease in the small bowel and tended to persist even in the absence of active inflammation. In contrast to Group A, this group occurred most frequently in small bowel disease, and least in colonic disease. Malabsorption was virtually confined to the patients with small bowel disease (10% incidence), while gallstones and renal stones were also both more frequent in Crohn's disease (11% and 9% respectively), the latter usually in association with small bowel resection or ileostomy. Group C, found in a small percentage of patients, consists of nonspecific complications, including osteoporosis (3%), liver disease (5%), peptic ulcer (10%), and amyloidosis (1%).", "contents": "The extra-intestinal complications of Crohn's disease and ulcerative colitis: a study of 700 patients. The records of a series of 700 patients with inflammatory bowel disease, 498 with Crohn's disease and 202 with ulcerative colitis, have been analyzed to determine the relative incidence and characteristic features of their extra-intestinal manifestations. The group with Crohn's disease included 62 with colitis, 223 with ileocolitis, and 213 with regional enteritis. A consideration of the clinical patterns and an understanding of their pathophysiology suggested a subdivision into two main groups: one \"colitis related\" and one related to the pathophysiology of the small nonspecific third group. Group A, colitis related, comprises joint, skin, mouth, and eye disease. The complications might be immunologically determined, were closely associated with active inflammation, and often responded to medical or surgical treatment of the underlying bowel disease. They occurred in 36% of the entire series of patients: joints were involved in 23%, skin in 15%, and mouth and eye each in 4%. Pyoderma gangrenosum was observed most often in ulcerative colitis and erythema nodosum most often in granulomatous colitis. The incidence of Group A complications was higher in disease involving the colon (42%) than in disease restricted exclusively to the small bowel (23%). There were interrelationships among the various members of Group A, with multiple manifestations occurring in a third of affected patients. Group B, related to small bowel pathophysiology, includes malabsorption, gallstones, kidney stones, and non-calculous hydronephrosis and hydroureter. Disorders in this group were generally related to the severity of the disease in the small bowel and tended to persist even in the absence of active inflammation. In contrast to Group A, this group occurred most frequently in small bowel disease, and least in colonic disease. Malabsorption was virtually confined to the patients with small bowel disease (10% incidence), while gallstones and renal stones were also both more frequent in Crohn's disease (11% and 9% respectively), the latter usually in association with small bowel resection or ileostomy. Group C, found in a small percentage of patients, consists of nonspecific complications, including osteoporosis (3%), liver disease (5%), peptic ulcer (10%), and amyloidosis (1%).", "PMID": 957999}
{"id": "pubmed23n0032_2936", "title": "Improvement of growth hormone response to stimulation in primary aldosteronism with correction of potassium deficiency.", "content": "Potassium depletion frequently occurs in primary aldosteronism and has been implicated as the cause of the impaired carbohydrate tolerance frequently associated with this syndrome. Glucose, insulin, and growth hormone regulation were studied in a 42-yr-old, male patient with an aldosterone-secreting adenoma when the patient was potassium-depleted and again after potassium repletion. Potassium repletion was documented by serial body potassium measurements, with an increase in body potassium from 2400 mEq to 2850 mEq after 400 mg spironolactone and 80 mEq supplemental potassium chloride were administered daily for 7 days. Potassium repletion resulted in improvement of the patient's glucose tolerance test, with a decrease in the peak glucose level from 184 mg/100ml to 130 mg/100ml and an increase in the peak insulin level from 46 muU/ml to 85 muU/ml. Intravenous administration of arginine resulted in a subnormal insulin response of 28 muU/ml in the base-line test and an increase to 59 muU/ml after potassium stores were repleted. Growth hormone response to arginine infusion was also initially minimal at 12.5 ng/ml, increasing markedly to 26 ng/ml after potassium replenishment. Insulin-induced hypoglycemia resulted in a depressed growth hormone response of 8 ng/ml when the patient was potassium-deficient, but a normal response of 30 ng/ml after potassium repletion. These observations demonstrate that impairment of both insulin and growth hormone responses to stimulation occur in primary aldosteronism with potassium depletion. These abnormalities may be reversed by potassium repletion.", "contents": "Improvement of growth hormone response to stimulation in primary aldosteronism with correction of potassium deficiency. Potassium depletion frequently occurs in primary aldosteronism and has been implicated as the cause of the impaired carbohydrate tolerance frequently associated with this syndrome. Glucose, insulin, and growth hormone regulation were studied in a 42-yr-old, male patient with an aldosterone-secreting adenoma when the patient was potassium-depleted and again after potassium repletion. Potassium repletion was documented by serial body potassium measurements, with an increase in body potassium from 2400 mEq to 2850 mEq after 400 mg spironolactone and 80 mEq supplemental potassium chloride were administered daily for 7 days. Potassium repletion resulted in improvement of the patient's glucose tolerance test, with a decrease in the peak glucose level from 184 mg/100ml to 130 mg/100ml and an increase in the peak insulin level from 46 muU/ml to 85 muU/ml. Intravenous administration of arginine resulted in a subnormal insulin response of 28 muU/ml in the base-line test and an increase to 59 muU/ml after potassium stores were repleted. Growth hormone response to arginine infusion was also initially minimal at 12.5 ng/ml, increasing markedly to 26 ng/ml after potassium replenishment. Insulin-induced hypoglycemia resulted in a depressed growth hormone response of 8 ng/ml when the patient was potassium-deficient, but a normal response of 30 ng/ml after potassium repletion. These observations demonstrate that impairment of both insulin and growth hormone responses to stimulation occur in primary aldosteronism with potassium depletion. These abnormalities may be reversed by potassium repletion.", "PMID": 958001}
{"id": "pubmed23n0032_2937", "title": "Mechanism of action of phenethylbiguanide (phenformin) in man. III. interrelationship between ethanol and phenethylibiguanide (PBG) in normal and diabetic subjects.", "content": "A standard 4-hr ethanol infusion (236 mg/min) after a 3-day fast with and without phenformin (25 mg q.i.d.), with blood drawn every hour for 8 hr, was performed on five normal subjects, eight obese nondiabetics, seven obese chemical diabetics, and four nonobese diabetics. Control infusion induced in all subjects a decline in blood sugar levels during and/or after the alcohol challenge, with a parallel decrease in basal plasma insulin. Hypoglycemia and the decrease in insulin secretion were associated with increased plasma free fatty acid concentration. Addition of phenethylbiguanide (PBG) to the preparatory 3-day fast resulted in a greater drop in the blood glucose levels of the normal control subjects, obese and nonobese diabetics; in the obese nondiabetics, however, significantly lower degree of blood glucose decrease than control was elicited. Furthermore, obese nondiabetics altered their blood glucose-insulin interaction with apparent increased responsivess of the B cells of PBG. The results suggest that effects of phenformin on blood glucose levels are more dependent on the metabolic state of the patient than on a property of the drug itself.", "contents": "Mechanism of action of phenethylbiguanide (phenformin) in man. III. interrelationship between ethanol and phenethylibiguanide (PBG) in normal and diabetic subjects. A standard 4-hr ethanol infusion (236 mg/min) after a 3-day fast with and without phenformin (25 mg q.i.d.), with blood drawn every hour for 8 hr, was performed on five normal subjects, eight obese nondiabetics, seven obese chemical diabetics, and four nonobese diabetics. Control infusion induced in all subjects a decline in blood sugar levels during and/or after the alcohol challenge, with a parallel decrease in basal plasma insulin. Hypoglycemia and the decrease in insulin secretion were associated with increased plasma free fatty acid concentration. Addition of phenethylbiguanide (PBG) to the preparatory 3-day fast resulted in a greater drop in the blood glucose levels of the normal control subjects, obese and nonobese diabetics; in the obese nondiabetics, however, significantly lower degree of blood glucose decrease than control was elicited. Furthermore, obese nondiabetics altered their blood glucose-insulin interaction with apparent increased responsivess of the B cells of PBG. The results suggest that effects of phenformin on blood glucose levels are more dependent on the metabolic state of the patient than on a property of the drug itself.", "PMID": 958002}
{"id": "pubmed23n0032_2938", "title": "Plasma catecholamine concentrations in hyperthyroidism and hypothyroidism.", "content": "Using a modification of the fluorometric method of Anton and Sayre, we have measured the plasma epinephrine (E) and norepinephrine (NE) concentrations in patients with thyroid dysfunction. There was no significant difference in plasma E in hyperthyroid or hypothyroid subjects, the values being similar to those observed in normal subjects. There was a striking relationship between age and plasma NE in the euthyroid individuals (r = 0.685, p less than 0.001, n = 41). Observed plasma NE concentrations were similar in control subjects (21.05 +/- 1.6 ng/100 ml; mean +/- SEM) and hyperthyroid patients (22.33+/- 2.0 ng/100 ml). However, plasma NE was significantly increased in hypothyroidism (35.46 +/- 3.9 ng/100 ml; p less than 0.01) and remained statistically different when the age factor was excluded (31.31 +/- 2.67 ng/100 ml; p less than 0.025). There was no correlation between plasma NE and serum thyroxine (T4), free thyroxine (FT4), or triiodothyronine (T3), in any of the three groups studied. These data indicate that hyperthyroidism is accompanied by normal plasma NE concentrations and that hypothyroidism is associated with significantly increased plasma NE concentrations, possible in an attempt to compensate for the lack of thyroid hormones.", "contents": "Plasma catecholamine concentrations in hyperthyroidism and hypothyroidism. Using a modification of the fluorometric method of Anton and Sayre, we have measured the plasma epinephrine (E) and norepinephrine (NE) concentrations in patients with thyroid dysfunction. There was no significant difference in plasma E in hyperthyroid or hypothyroid subjects, the values being similar to those observed in normal subjects. There was a striking relationship between age and plasma NE in the euthyroid individuals (r = 0.685, p less than 0.001, n = 41). Observed plasma NE concentrations were similar in control subjects (21.05 +/- 1.6 ng/100 ml; mean +/- SEM) and hyperthyroid patients (22.33+/- 2.0 ng/100 ml). However, plasma NE was significantly increased in hypothyroidism (35.46 +/- 3.9 ng/100 ml; p less than 0.01) and remained statistically different when the age factor was excluded (31.31 +/- 2.67 ng/100 ml; p less than 0.025). There was no correlation between plasma NE and serum thyroxine (T4), free thyroxine (FT4), or triiodothyronine (T3), in any of the three groups studied. These data indicate that hyperthyroidism is accompanied by normal plasma NE concentrations and that hypothyroidism is associated with significantly increased plasma NE concentrations, possible in an attempt to compensate for the lack of thyroid hormones.", "PMID": 958003}
{"id": "pubmed23n0032_2939", "title": "Studies on obesity. III. effect of triiodothyronine (T3) on thyroglobulin autoantibodies in euthyroid obese subjects.", "content": "Effect of T3 therapy on tanned red cell agglutinating thyroglobulin (TRC-TG) antibodies in 10 obese subjects without apparent thyroid disease was investigated. Six other obese subjects without thyroid dysfunction and of approximately the same mean age who also had circulating TRC-TG antibodies served as control subjects and were untreated. In vitro thyroid tests (TSH, total and free T4) performed before T3 therapy, as well as clinical examination, showed thyroid function to be normal in all subjects, and there was no evidence of thyroiditis. TRC-TG antibodies were present in low to moderate titers of 40-1280 in control subjects as well as in subjects selected for T3 treatment. Therapy with T3 was started at 50 mug/day and gradually increased to a maximum of 250 mug/day, depending on clinical needs. T3-treated as well as untreated obese control subjects were all maintained on a high protein, low fat, low carbohydrate diet. Duration of T3 therapy varied from 2-8 mo, and in all but one T3-treated subject, TRC-TG antibodies completely disappeared. In the one exceptional case, TRC-TG antibody titer decreased from 1280 to 80 after 7 mo of therapy. In non-T3-treated obese control subjects, antibody titers remained at the same levels throughout the observation period, thereby indicating a lack of spontaneous regression of circulating immune response. Therapy with T3, by inhibiting TSH, may have caused regression of inapparent immunologic thyroid lesion, thus leading to the disappearance of circulating TRC antibodies; alternatively, T3 specifically may have accelerated catabolism of thyroid antibodies. The latter possibility is favored in the absence of clinical and laboratory evidence of thyroiditis in T3-treated subjects.", "contents": "Studies on obesity. III. effect of triiodothyronine (T3) on thyroglobulin autoantibodies in euthyroid obese subjects. Effect of T3 therapy on tanned red cell agglutinating thyroglobulin (TRC-TG) antibodies in 10 obese subjects without apparent thyroid disease was investigated. Six other obese subjects without thyroid dysfunction and of approximately the same mean age who also had circulating TRC-TG antibodies served as control subjects and were untreated. In vitro thyroid tests (TSH, total and free T4) performed before T3 therapy, as well as clinical examination, showed thyroid function to be normal in all subjects, and there was no evidence of thyroiditis. TRC-TG antibodies were present in low to moderate titers of 40-1280 in control subjects as well as in subjects selected for T3 treatment. Therapy with T3 was started at 50 mug/day and gradually increased to a maximum of 250 mug/day, depending on clinical needs. T3-treated as well as untreated obese control subjects were all maintained on a high protein, low fat, low carbohydrate diet. Duration of T3 therapy varied from 2-8 mo, and in all but one T3-treated subject, TRC-TG antibodies completely disappeared. In the one exceptional case, TRC-TG antibody titer decreased from 1280 to 80 after 7 mo of therapy. In non-T3-treated obese control subjects, antibody titers remained at the same levels throughout the observation period, thereby indicating a lack of spontaneous regression of circulating immune response. Therapy with T3, by inhibiting TSH, may have caused regression of inapparent immunologic thyroid lesion, thus leading to the disappearance of circulating TRC antibodies; alternatively, T3 specifically may have accelerated catabolism of thyroid antibodies. The latter possibility is favored in the absence of clinical and laboratory evidence of thyroiditis in T3-treated subjects.", "PMID": 958004}
{"id": "pubmed23n0032_2940", "title": "Control of calcium absorption: effect of sodium chloride loading and depletion.", "content": "In six out of eight patients with normal parathyroid function and in two subjects with hypoparathyroidism, sodium chloride loading was shown to increase calcium absorption as measured by the 47Ca absorption test. Although no significant change was seen in the renal and fecal excretion of calcium, a slight decrease in total serum calcium did occur with increased sodium intake. The change in calcium absorption was not accompanied by a detectable change in calcium balance. Therefore, a compensatory change in gastrointestinal calcium secretion must have occurred. Sodium intake has an effect on calcium in the gastrointestinal tract, which is independent of parathyroid hormone. Sodium intake should be considered when results of studies of calcium metabolism are compared.", "contents": "Control of calcium absorption: effect of sodium chloride loading and depletion. In six out of eight patients with normal parathyroid function and in two subjects with hypoparathyroidism, sodium chloride loading was shown to increase calcium absorption as measured by the 47Ca absorption test. Although no significant change was seen in the renal and fecal excretion of calcium, a slight decrease in total serum calcium did occur with increased sodium intake. The change in calcium absorption was not accompanied by a detectable change in calcium balance. Therefore, a compensatory change in gastrointestinal calcium secretion must have occurred. Sodium intake has an effect on calcium in the gastrointestinal tract, which is independent of parathyroid hormone. Sodium intake should be considered when results of studies of calcium metabolism are compared.", "PMID": 958005}
{"id": "pubmed23n0032_2941", "title": "Changing attitudes of medical teachers towards medical education.", "content": "If medical education is to improve, this is largely dependent upon the attitudes of clinical teachers towards their own teaching and their students' learning. The aims and objectives of our workshop method of medical teacher training therefore includes some in the affective domain. Based on the theory of cognitive dissonance the experience-linked practical problem solving approach of the workshop encourages behaviour change, presuming this to cause 'attitude change'. Testing by means of a specially constructed and validated Likert-type attitude scale shows that attitudes do improve significantly and consistently over the 5 days of our workshops. A follow-up study shows these effects to be maintained and taken back to the participants' own medical schools.", "contents": "Changing attitudes of medical teachers towards medical education. If medical education is to improve, this is largely dependent upon the attitudes of clinical teachers towards their own teaching and their students' learning. The aims and objectives of our workshop method of medical teacher training therefore includes some in the affective domain. Based on the theory of cognitive dissonance the experience-linked practical problem solving approach of the workshop encourages behaviour change, presuming this to cause 'attitude change'. Testing by means of a specially constructed and validated Likert-type attitude scale shows that attitudes do improve significantly and consistently over the 5 days of our workshops. A follow-up study shows these effects to be maintained and taken back to the participants' own medical schools.", "PMID": 958022}
{"id": "pubmed23n0032_2942", "title": "A comparative study of teachers' attitudes in the teaching of undergraduate medical students.", "content": "Senior staff of the departments of medicine, surgery and general practice in each medical school in the United Kingdom were asked to annotate the same case studies which had been prepared by medical students. Analysis of the annotations showed that general practitioners demanded much greater consideration by the student of the patient, his family and his environment, whereas physicians and surgeons tended to be more disease orientated. Nevertheless, expectations and attitudes of teachers in similar departments are remarkably diverse and this highlighted the potential role of the case study as an educational tool for staff and students alike. Many departments need to clarify the difference between undergraduate and postgraduate teaching objectives.", "contents": "A comparative study of teachers' attitudes in the teaching of undergraduate medical students. Senior staff of the departments of medicine, surgery and general practice in each medical school in the United Kingdom were asked to annotate the same case studies which had been prepared by medical students. Analysis of the annotations showed that general practitioners demanded much greater consideration by the student of the patient, his family and his environment, whereas physicians and surgeons tended to be more disease orientated. Nevertheless, expectations and attitudes of teachers in similar departments are remarkably diverse and this highlighted the potential role of the case study as an educational tool for staff and students alike. Many departments need to clarify the difference between undergraduate and postgraduate teaching objectives.", "PMID": 958023}
{"id": "pubmed23n0032_2943", "title": "The organization of a postgraduate training course for medical advisers in the pharmaceutical industry.", "content": "A postgraduate training and education course for physicians working in the field of pharmaceutical medicine is described. The course proved welcome and acceptable to the participants and provided valuable experience for the organizers. The scientific content and information gained in organizing the course have been incorporated in the planning of a postgraduate diploma course in pharmaceutical medicine.", "contents": "The organization of a postgraduate training course for medical advisers in the pharmaceutical industry. A postgraduate training and education course for physicians working in the field of pharmaceutical medicine is described. The course proved welcome and acceptable to the participants and provided valuable experience for the organizers. The scientific content and information gained in organizing the course have been incorporated in the planning of a postgraduate diploma course in pharmaceutical medicine.", "PMID": 958024}
{"id": "pubmed23n0032_2944", "title": "Preclinical students' views on knowing cancer diagnosis: a data-based class exercise.", "content": "One way of tackling the problems of demonstrating the relevance of psychology to medical practice as well as to medically-inexperienced preclinical students is to elicit their views on topical issues and present them with a comparison of their own data and any available in the medical literature. In a survey of preclinical students' views on being told their diagnosis and prognosis in the hypothetical case of cancer, high agreement in the proportions wanting to be or not to be told was found between the responses of students and a sample of cancer patients reported by Kelly & Friesen (1950). The exercise served to demonstrate that the 'to tell or not to tell' debate is one in which students can also be involved.", "contents": "Preclinical students' views on knowing cancer diagnosis: a data-based class exercise. One way of tackling the problems of demonstrating the relevance of psychology to medical practice as well as to medically-inexperienced preclinical students is to elicit their views on topical issues and present them with a comparison of their own data and any available in the medical literature. In a survey of preclinical students' views on being told their diagnosis and prognosis in the hypothetical case of cancer, high agreement in the proportions wanting to be or not to be told was found between the responses of students and a sample of cancer patients reported by Kelly & Friesen (1950). The exercise served to demonstrate that the 'to tell or not to tell' debate is one in which students can also be involved.", "PMID": 958025}
{"id": "pubmed23n0032_2945", "title": "Instruction on death and dying for the medical student.", "content": "With instruction on death and dying beginning to be included in the curricula of many medical schools and the likelihood that this development will spread, it is timely to examine the best ways in which the subject can be taught. The most important need at this stage is the creation of objectives. Nine objectives have been briefly outlined which have either been reported on by teachers in the field or which seem to be pertinent to death education. The focus on objectives rests on the premise that the format and content of courses will follow largely from the goals that are selected. Questions of who should teach death and dying, when it should be taught and what format should be used, are discussed.", "contents": "Instruction on death and dying for the medical student. With instruction on death and dying beginning to be included in the curricula of many medical schools and the likelihood that this development will spread, it is timely to examine the best ways in which the subject can be taught. The most important need at this stage is the creation of objectives. Nine objectives have been briefly outlined which have either been reported on by teachers in the field or which seem to be pertinent to death education. The focus on objectives rests on the premise that the format and content of courses will follow largely from the goals that are selected. Questions of who should teach death and dying, when it should be taught and what format should be used, are discussed.", "PMID": 958026}
{"id": "pubmed23n0032_2946", "title": "Migration of Baroda medical graduates, 1949--72.", "content": "1. The migration of doctors from Medical College, Baroda, is studied in detail. 2. From 1949 to 1972, 584 doctors migrated to other countries and only twenty-nine doctors have returned so far. The incidence of migration is to the extent of 39-75%. 3. Among those who migrated 77-7% have gone to U.S.A. and 11-7% have gone to the U.K. 4. Ninety-four women graduates out of 241 passed prior to 1971 are abroad.. 5. The majority migrate for better and more comfortable living conditions. A small number migrate for better research facilities and encouragement. Those who are denied the opportunity of a post-graduate training also migrate. 6. Migration of doctors, though universal, is disturbingly high in India and a major cause of concern to the government and the educationist.", "contents": "Migration of Baroda medical graduates, 1949--72. 1. The migration of doctors from Medical College, Baroda, is studied in detail. 2. From 1949 to 1972, 584 doctors migrated to other countries and only twenty-nine doctors have returned so far. The incidence of migration is to the extent of 39-75%. 3. Among those who migrated 77-7% have gone to U.S.A. and 11-7% have gone to the U.K. 4. Ninety-four women graduates out of 241 passed prior to 1971 are abroad.. 5. The majority migrate for better and more comfortable living conditions. A small number migrate for better research facilities and encouragement. Those who are denied the opportunity of a post-graduate training also migrate. 6. Migration of doctors, though universal, is disturbingly high in India and a major cause of concern to the government and the educationist.", "PMID": 958028}
{"id": "pubmed23n0032_2947", "title": "Performance of women medical graduates from Medical College, Baroda, 1949--74.", "content": "Women account for 15% of the total admissions in medicine. 271 women have graduated from Medical College, Baroda in 25 years. The examination results are better in women than in men. Obstetrics and gynaecology is the most common speciality selected by women. Job satisfaction is found in 87% of women. Unmarried women account for 15%, 66% have married a doctor and 19% are married to non-medical spouse. Emigration is to the extent of 39%.", "contents": "Performance of women medical graduates from Medical College, Baroda, 1949--74. Women account for 15% of the total admissions in medicine. 271 women have graduated from Medical College, Baroda in 25 years. The examination results are better in women than in men. Obstetrics and gynaecology is the most common speciality selected by women. Job satisfaction is found in 87% of women. Unmarried women account for 15%, 66% have married a doctor and 19% are married to non-medical spouse. Emigration is to the extent of 39%.", "PMID": 958029}
{"id": "pubmed23n0032_2948", "title": "Married women doctors' training and re-employment schemes in the West Midlands Region, with particular reference to psychiatry.", "content": "The response of one region to the Department of Health and Social Security recommendations on re-employment of women doctors contained in H.M. 69(6) is described. In many specialities there has been an encouraging return of married women doctors to medical practice within the hospital service. In the field of psychiatry the experience in a local sub-regional training scheme suggests that graduates of a high calibre can be attracted back to this speciality on a part-time basis for training; the training has been acceptable for full qualification and the examination success rate is high; part-time appointments, including senior registrar and consultant posts, can be obtained; it would not appear that training need, in the first instance, be confined to those people working in a main teaching centre; the satisfactory resolution of any conflict between trainees' domestic and professional commitments is important, as is a flexible approach to the individual professional needs of the trainees. Such schemes appear to tap a valuable source of potential manpower which higherto has not been fully utilized.", "contents": "Married women doctors' training and re-employment schemes in the West Midlands Region, with particular reference to psychiatry. The response of one region to the Department of Health and Social Security recommendations on re-employment of women doctors contained in H.M. 69(6) is described. In many specialities there has been an encouraging return of married women doctors to medical practice within the hospital service. In the field of psychiatry the experience in a local sub-regional training scheme suggests that graduates of a high calibre can be attracted back to this speciality on a part-time basis for training; the training has been acceptable for full qualification and the examination success rate is high; part-time appointments, including senior registrar and consultant posts, can be obtained; it would not appear that training need, in the first instance, be confined to those people working in a main teaching centre; the satisfactory resolution of any conflict between trainees' domestic and professional commitments is important, as is a flexible approach to the individual professional needs of the trainees. Such schemes appear to tap a valuable source of potential manpower which higherto has not been fully utilized.", "PMID": 958030}
{"id": "pubmed23n0032_2949", "title": "Constructing a new course for undergraduate teaching of general practice.", "content": "This paper outlines in Part I the method used to analyse and re-define a set of educational objectives on which a new course of teaching of general practice in the later clinical years is based. Part II describes the course, the learning methods, behavioural attainments and the techniques of evaluation to be implemented. A year was spent on the exercise by a group which comprised the professorial head, consultant senior lecturer and a senior registrar tutor from the Department of General Practice, Queen's University, Belfast, and a consultant educationalist, a senior lecturer, Department of Education, of the same University.", "contents": "Constructing a new course for undergraduate teaching of general practice. This paper outlines in Part I the method used to analyse and re-define a set of educational objectives on which a new course of teaching of general practice in the later clinical years is based. Part II describes the course, the learning methods, behavioural attainments and the techniques of evaluation to be implemented. A year was spent on the exercise by a group which comprised the professorial head, consultant senior lecturer and a senior registrar tutor from the Department of General Practice, Queen's University, Belfast, and a consultant educationalist, a senior lecturer, Department of Education, of the same University.", "PMID": 958031}
{"id": "pubmed23n0032_2950", "title": "General practice teaching--within the hospital.", "content": "A programme of integrated teaching by consultants and general practitioners is described. The teaching took place in the hospitals used for the purpose by the Medical Faculty of the University of Birmingham.", "contents": "General practice teaching--within the hospital. A programme of integrated teaching by consultants and general practitioners is described. The teaching took place in the hospitals used for the purpose by the Medical Faculty of the University of Birmingham.", "PMID": 958032}
{"id": "pubmed23n0032_2951", "title": "Fluid exchanges through capillary walls: a modification of the Starling hypothesis.", "content": "In textbooks of physiology it is usually stated that fluids are extravasated from the arterial ends of capillaries, because, there, the outward transmural pressure of the blood exceeds its net inward osmotic pressure. At the venous ends, however, the position is reversed. The fluids are, therefore, reabsorbed. Inthis paper it is argued that this is not a true picture of what must really happen. For, having once become equal there is no reason why the pressures should ever cease to be so. Instead, fluids are absorbed at exactly that rate which just keeps pace with falling hydraulic pressure, as the blood proceeds along the capillary loop. Moreover, the forces are indeed shown to be equal at the extreme venous ends of the capillaries. It is further argued that, though this mechanism might operate anywhere in the horizontal position, it could not possibly work in the feet of a standing man. For here the hydraulic blood pressure everywhere greatly exceeds the osmotic pressure of plasma proteins. An addition to Starling's hypothesis is therefore suggested to adapt it to dependent tissues. An experiment by which the validity of this addition might be tested is described.", "contents": "Fluid exchanges through capillary walls: a modification of the Starling hypothesis. In textbooks of physiology it is usually stated that fluids are extravasated from the arterial ends of capillaries, because, there, the outward transmural pressure of the blood exceeds its net inward osmotic pressure. At the venous ends, however, the position is reversed. The fluids are, therefore, reabsorbed. Inthis paper it is argued that this is not a true picture of what must really happen. For, having once become equal there is no reason why the pressures should ever cease to be so. Instead, fluids are absorbed at exactly that rate which just keeps pace with falling hydraulic pressure, as the blood proceeds along the capillary loop. Moreover, the forces are indeed shown to be equal at the extreme venous ends of the capillaries. It is further argued that, though this mechanism might operate anywhere in the horizontal position, it could not possibly work in the feet of a standing man. For here the hydraulic blood pressure everywhere greatly exceeds the osmotic pressure of plasma proteins. An addition to Starling's hypothesis is therefore suggested to adapt it to dependent tissues. An experiment by which the validity of this addition might be tested is described.", "PMID": 958036}
{"id": "pubmed23n0032_2952", "title": "Biological function of ascorbic acid and the pathogenesis of scurvy: a working hypothesis.", "content": "This communication advances the proposal that all the diverse manifestations of scurvy can be attributed to depolymerisation of ground substance glycosaminoglycans brought about by exposure to uninhibited cellular hyaluronidase. It suggests that ascorbic acid exerts its prime biological function indirectly by incorporation into a glycosaminoglycan residue to form the physiological hyaluronidase inhibitor. The therapeutic implications of this working hypothesis are briefly discussed.", "contents": "Biological function of ascorbic acid and the pathogenesis of scurvy: a working hypothesis. This communication advances the proposal that all the diverse manifestations of scurvy can be attributed to depolymerisation of ground substance glycosaminoglycans brought about by exposure to uninhibited cellular hyaluronidase. It suggests that ascorbic acid exerts its prime biological function indirectly by incorporation into a glycosaminoglycan residue to form the physiological hyaluronidase inhibitor. The therapeutic implications of this working hypothesis are briefly discussed.", "PMID": 958038}
{"id": "pubmed23n0032_2953", "title": "Clinical method is all wrong.", "content": "Two models of diagnostic clinical behaviour are studied: the conventional in which data gathering precedes interpretation, and the sequential in which each item is evaluated as it is acquired and incorporated into decision making, usually of binary type. The second model is proposed as that more closely related to the reality of clinical practice and also as potentially more capable of exploiting intellectual resources and creating logistic economies.", "contents": "Clinical method is all wrong. Two models of diagnostic clinical behaviour are studied: the conventional in which data gathering precedes interpretation, and the sequential in which each item is evaluated as it is acquired and incorporated into decision making, usually of binary type. The second model is proposed as that more closely related to the reality of clinical practice and also as potentially more capable of exploiting intellectual resources and creating logistic economies.", "PMID": 958039}
{"id": "pubmed23n0032_2954", "title": "Folate deficiency and neurological disorders in adults.", "content": "The restless legs syndrome could represent a folate responsive disorder in both patients with acquired-folate deficiency and those with familial symptomatology. Patients with acquired folate-deficiency could be divided into two subgroups. (i) those with minor neurological signs (restless legs syndrome, vibration sense impairment and tactile hypoesthesia in both legs with diminished ankle jerks and a prolonged or assymetrical Achilles-reflex time) and (ii) those with major neurological signs (subacute combined degeneration with or without neuropathies). In some of these patients the classical triad of the malabsorption syndrome is replaced by another triad, constipation, abnormal jejunal biopsy and abnormal d-xylose absorption. A low folic serum acid level could induce minor neuropsychiatric symptoms while an additional low CSF folate could induce major neurological symptoms in spite of the presence of a normal erythrocyte folate level and in the absence of frank anemia. Possible further studies are described.", "contents": "Folate deficiency and neurological disorders in adults. The restless legs syndrome could represent a folate responsive disorder in both patients with acquired-folate deficiency and those with familial symptomatology. Patients with acquired folate-deficiency could be divided into two subgroups. (i) those with minor neurological signs (restless legs syndrome, vibration sense impairment and tactile hypoesthesia in both legs with diminished ankle jerks and a prolonged or assymetrical Achilles-reflex time) and (ii) those with major neurological signs (subacute combined degeneration with or without neuropathies). In some of these patients the classical triad of the malabsorption syndrome is replaced by another triad, constipation, abnormal jejunal biopsy and abnormal d-xylose absorption. A low folic serum acid level could induce minor neuropsychiatric symptoms while an additional low CSF folate could induce major neurological symptoms in spite of the presence of a normal erythrocyte folate level and in the absence of frank anemia. Possible further studies are described.", "PMID": 958035}
{"id": "pubmed23n0032_2955", "title": "Is there a protein ligase?", "content": "Results obtained from experiments dealing with mammalian, bacterial, phage and mitochondrial protein biosynthesis as well as certain enzymatically performed amino acid replacement studies on Kunitz trypsin inhibitor strongly suggest that protein ligation may be occuring in vivo. Amino acid substitution experiments prove the reversibility of endopeptidase reactions, and protein ligation is the reverse of endopeptidase reaction. These experiments are discussed in detail and the suggestion is made that ligation may also be useful in the repair of certain essential proteins which may become damaged.", "contents": "Is there a protein ligase? Results obtained from experiments dealing with mammalian, bacterial, phage and mitochondrial protein biosynthesis as well as certain enzymatically performed amino acid replacement studies on Kunitz trypsin inhibitor strongly suggest that protein ligation may be occuring in vivo. Amino acid substitution experiments prove the reversibility of endopeptidase reactions, and protein ligation is the reverse of endopeptidase reaction. These experiments are discussed in detail and the suggestion is made that ligation may also be useful in the repair of certain essential proteins which may become damaged.", "PMID": 958037}
{"id": "pubmed23n0032_2956", "title": "A newly developed therapeutic surgical and cardiac intensive care monitor.", "content": "A computerized intensive care monitor patterned after a clinical system operating at the University of Alabama has been developed. The system, which has been operating in an intensive care unit since February 1973, is equipped with conventional bedside biomedical instrumentation, special-purpose devices, and keyboard/display terminals interfaced with a minicomputer. Measurement of vital parameters as well as the automatic infusion of blood controlled by the computer in a closed loop feedback mode is available. Communication with the computer via the bedside terminals permits the display and retrieval of clinical data, entry of blood gas measurements and pressure limits for blood infusion, the revision of measurement status, and the control of the computer in measuring cardiac output. The administration of blood and intravenous fluid may be achieved under computer or manual option.", "contents": "A newly developed therapeutic surgical and cardiac intensive care monitor. A computerized intensive care monitor patterned after a clinical system operating at the University of Alabama has been developed. The system, which has been operating in an intensive care unit since February 1973, is equipped with conventional bedside biomedical instrumentation, special-purpose devices, and keyboard/display terminals interfaced with a minicomputer. Measurement of vital parameters as well as the automatic infusion of blood controlled by the computer in a closed loop feedback mode is available. Communication with the computer via the bedside terminals permits the display and retrieval of clinical data, entry of blood gas measurements and pressure limits for blood infusion, the revision of measurement status, and the control of the computer in measuring cardiac output. The administration of blood and intravenous fluid may be achieved under computer or manual option.", "PMID": 958047}
{"id": "pubmed23n0032_2957", "title": "Endocardial electrograms and pacemaker sensing.", "content": "Endocardial electrograms in 77 acute cases (at time of pacemaker implant) and 56 chronic cases (at time of pulse generator replacement, with electrodes in service for at least 6 months) were recorded. The peak-to-peak voltage and rate of rise, or slew rate, were compared acutely and chronically. Both parameters must simultaneously exceed given thresholds to trigger the sensing circuits of implantable pacemakers. The average chronic slew rate was half the average acute value. The maximum slew rate and voltage swing occur during the intrinsic deflection as a virtually straight line segment of the electrogram. The findings are consistent with the predictions of a mathematical model for genesis of the electrogram, and also with a model for explaining acute-to-chronic changes in stimulation current thresholds.", "contents": "Endocardial electrograms and pacemaker sensing. Endocardial electrograms in 77 acute cases (at time of pacemaker implant) and 56 chronic cases (at time of pulse generator replacement, with electrodes in service for at least 6 months) were recorded. The peak-to-peak voltage and rate of rise, or slew rate, were compared acutely and chronically. Both parameters must simultaneously exceed given thresholds to trigger the sensing circuits of implantable pacemakers. The average chronic slew rate was half the average acute value. The maximum slew rate and voltage swing occur during the intrinsic deflection as a virtually straight line segment of the electrogram. The findings are consistent with the predictions of a mathematical model for genesis of the electrogram, and also with a model for explaining acute-to-chronic changes in stimulation current thresholds.", "PMID": 958042}
{"id": "pubmed23n0032_2958", "title": "Hydrogen-induced microelectronic capacitor failure in pacemakers.", "content": "Ceramic chip capacitors used in hybrid microelectronics for cardiac pacemakers are usually highly reliable. However, under certain conditions of capacitor construction, capacitor materials, mounting techniques, and environmental conditions, high failure rates may occur. A specific example is presented in which a ceramic capacitor used in an implanted pacemaker delaminated and failed approximately 30 days after being implanted. The failed capacitor caused a pulse rate rise, but due to circuit design techniques, the rate increase was limited to an acceptable value. The capacitor that failed was from an isolated lot of capacitors that was manufactured using pure palladium plates. The circuit containing this capacitor was hermetically sealed within a titanium case by welding. During the welding, a small amount of hydrogen was released from the titanium which, over a period of 2 to 4 weeks, was absorbed by the palladium plates in the capacitor. By absorbing the hydrogen, the palladium plates exhibit a volumetric expansion of sufficient magnitude to crack and delaminate the capacitor to the point of failure. Subsequently, the recurrence of this failure mode has been avoided by using capacitors containing special palladium alloys that cannot absorb hydrogen. This phenomenon is of interest to pacemaker designers since mercury batteries used in conventional pacemakers generate large amounts of hydrogen and potentially may be responsible for complications when used in conjunction with capacitors containing palladium.", "contents": "Hydrogen-induced microelectronic capacitor failure in pacemakers. Ceramic chip capacitors used in hybrid microelectronics for cardiac pacemakers are usually highly reliable. However, under certain conditions of capacitor construction, capacitor materials, mounting techniques, and environmental conditions, high failure rates may occur. A specific example is presented in which a ceramic capacitor used in an implanted pacemaker delaminated and failed approximately 30 days after being implanted. The failed capacitor caused a pulse rate rise, but due to circuit design techniques, the rate increase was limited to an acceptable value. The capacitor that failed was from an isolated lot of capacitors that was manufactured using pure palladium plates. The circuit containing this capacitor was hermetically sealed within a titanium case by welding. During the welding, a small amount of hydrogen was released from the titanium which, over a period of 2 to 4 weeks, was absorbed by the palladium plates in the capacitor. By absorbing the hydrogen, the palladium plates exhibit a volumetric expansion of sufficient magnitude to crack and delaminate the capacitor to the point of failure. Subsequently, the recurrence of this failure mode has been avoided by using capacitors containing special palladium alloys that cannot absorb hydrogen. This phenomenon is of interest to pacemaker designers since mercury batteries used in conventional pacemakers generate large amounts of hydrogen and potentially may be responsible for complications when used in conjunction with capacitors containing palladium.", "PMID": 958043}
{"id": "pubmed23n0032_2959", "title": "A diameter-independent blood flow measurement technique.", "content": "A phase difference technique for measuring true volume flow rate has been developed. The concept utilizes the phase difference from unequal transit times between bursts of ultrasonic energy transmitted simultaneously against and with the blood flow. A theoretical analysis reveals that the volume flow rate sensitivity (V/ml/sec) for a point crystal illumination pattern directed diagonally through the center of the vessel is a function of the inverse of the diameter. But an illumination pattern orientated perpendicularly to the vessel axis, which spans the entire diameter of the vessel, yields a volume flow rate sensitivity independent of the diameter. Line illumination, in contrast to point illumination, does not require the vessel diameter to be measured in order to establish the sensitivity factor, and a change in diameter does not alter the sensitivity factor. Line illumination has a considerable advantage when used on small vessels in which the ratio of wall thickness to the lumen diameter is large and is often variable.", "contents": "A diameter-independent blood flow measurement technique. A phase difference technique for measuring true volume flow rate has been developed. The concept utilizes the phase difference from unequal transit times between bursts of ultrasonic energy transmitted simultaneously against and with the blood flow. A theoretical analysis reveals that the volume flow rate sensitivity (V/ml/sec) for a point crystal illumination pattern directed diagonally through the center of the vessel is a function of the inverse of the diameter. But an illumination pattern orientated perpendicularly to the vessel axis, which spans the entire diameter of the vessel, yields a volume flow rate sensitivity independent of the diameter. Line illumination, in contrast to point illumination, does not require the vessel diameter to be measured in order to establish the sensitivity factor, and a change in diameter does not alter the sensitivity factor. Line illumination has a considerable advantage when used on small vessels in which the ratio of wall thickness to the lumen diameter is large and is often variable.", "PMID": 958044}
{"id": "pubmed23n0032_2960", "title": "Semi-invasive method for instantaneous and phasic measurement of arterial blood pressure.", "content": "A method for the instantaneous and phasic measurement of arterial blood pressure that eliminates the necessity of entry of the artery is described. This is accomplished with a miniature strain gauge transducer enclosed within a plastic cuff which is positioned around the artery. The device is suitable for monitoring arterial pressure in seriously ill patients and for chronic measurements of pressure in untrained and unanesthetized laboratory animals. The system was found to be stable, and recorded pressures were virtually identical to those measured intra-arterially with a catheter-tip micromanometer.", "contents": "Semi-invasive method for instantaneous and phasic measurement of arterial blood pressure. A method for the instantaneous and phasic measurement of arterial blood pressure that eliminates the necessity of entry of the artery is described. This is accomplished with a miniature strain gauge transducer enclosed within a plastic cuff which is positioned around the artery. The device is suitable for monitoring arterial pressure in seriously ill patients and for chronic measurements of pressure in untrained and unanesthetized laboratory animals. The system was found to be stable, and recorded pressures were virtually identical to those measured intra-arterially with a catheter-tip micromanometer.", "PMID": 958045}
{"id": "pubmed23n0032_2961", "title": "A prospective evaluation of open prefrontal leucotomy.", "content": "Forty-three patients received an open prefrontal leucotomy for severe and intractable psychiatric illnesses. As a result of the operation three patients died, three developed personality changes and one had repeated grand-mal seizures. Of the 40 patients followed up for six months, 57-5% showed marked improvement in their clinical state and 30-0% mild to moderate degrees of improvement whilst no patient's condition was considered to be worse. Significant improvement was obtained after operation in the mean scores on the Hamilton Anxiety Scale, the Hamilton Depression Scale, the Beck Depression Scale and the Neuroticism Scale of the Eysenck Personality Inventory. Extroversion, as measured by the Eysenck Personality Inventory, was significantly increased after operation. It is recommended that open prefrontal leucotomy procedures be replaced by the safer stereotactic or electrode implantation techniques and that all psychosurgery be confined to specialist units.", "contents": "A prospective evaluation of open prefrontal leucotomy. Forty-three patients received an open prefrontal leucotomy for severe and intractable psychiatric illnesses. As a result of the operation three patients died, three developed personality changes and one had repeated grand-mal seizures. Of the 40 patients followed up for six months, 57-5% showed marked improvement in their clinical state and 30-0% mild to moderate degrees of improvement whilst no patient's condition was considered to be worse. Significant improvement was obtained after operation in the mean scores on the Hamilton Anxiety Scale, the Hamilton Depression Scale, the Beck Depression Scale and the Neuroticism Scale of the Eysenck Personality Inventory. Extroversion, as measured by the Eysenck Personality Inventory, was significantly increased after operation. It is recommended that open prefrontal leucotomy procedures be replaced by the safer stereotactic or electrode implantation techniques and that all psychosurgery be confined to specialist units.", "PMID": 958061}
{"id": "pubmed23n0032_2962", "title": "An analysis of 340 cases of syphilis diagnosed in the laboratory in six months in 1973.", "content": "Between July and December, 1973, 340 new cases of syphilis previously unknown to the laboratory were diagnosed on the results of serological tests. The male-female ratio was 2-5 : 1. Homosexually acquired infections accounted for 34-3% of cases of syphilis in males. The cases were grouped into primary, secondary, latent and late stages of syphilis according to history, clinical signs and serological test results. Cases in each stage were subdivided into age groups. Primary and secondary syphilis (that is, the early infectious stages) occurred in 56 males and 23 females under 29 years of age. In 41 of the males (73-2%) this infection was homosexually acquired. The figures from this analysis show similar trends to those available in the United Kingdom.", "contents": "An analysis of 340 cases of syphilis diagnosed in the laboratory in six months in 1973. Between July and December, 1973, 340 new cases of syphilis previously unknown to the laboratory were diagnosed on the results of serological tests. The male-female ratio was 2-5 : 1. Homosexually acquired infections accounted for 34-3% of cases of syphilis in males. The cases were grouped into primary, secondary, latent and late stages of syphilis according to history, clinical signs and serological test results. Cases in each stage were subdivided into age groups. Primary and secondary syphilis (that is, the early infectious stages) occurred in 56 males and 23 females under 29 years of age. In 41 of the males (73-2%) this infection was homosexually acquired. The figures from this analysis show similar trends to those available in the United Kingdom.", "PMID": 958062}
{"id": "pubmed23n0032_2963", "title": "Radioimmunoassay as an improved method for measurement of serum levels of gentamicin.", "content": "A radioimmunoassay is described for the measurement of serum concentrations of gentamicin. This radioimmunoassay is more rapid, more specific and more accurate than microbiological assays. Knowledge of serum levels of gentamicin assists the clinician in regulating drug dosage to obtain an optimal therapeutic effect, and yet avoid toxic serum levels.", "contents": "Radioimmunoassay as an improved method for measurement of serum levels of gentamicin. A radioimmunoassay is described for the measurement of serum concentrations of gentamicin. This radioimmunoassay is more rapid, more specific and more accurate than microbiological assays. Knowledge of serum levels of gentamicin assists the clinician in regulating drug dosage to obtain an optimal therapeutic effect, and yet avoid toxic serum levels.", "PMID": 958063}
{"id": "pubmed23n0032_2964", "title": "Vasectomy.", "content": "Fifty consecutive patients who underwent vasectomy were followed up over a period of two years, and were subjected to a questionnaire, and the results were analysed. The technique for vasectomy is described and a few modifications are suggested, so that there is a reasonable chance of reversing the operation if need be. Special emphasis has been placed on the poorly understood medicolegal implications of vasectomy.", "contents": "Vasectomy. Fifty consecutive patients who underwent vasectomy were followed up over a period of two years, and were subjected to a questionnaire, and the results were analysed. The technique for vasectomy is described and a few modifications are suggested, so that there is a reasonable chance of reversing the operation if need be. Special emphasis has been placed on the poorly understood medicolegal implications of vasectomy.", "PMID": 958064}
{"id": "pubmed23n0032_2965", "title": "Microsporum nanum infection in South Australia.", "content": "Two cases of human ringworm caused by the dermatophyte Microsporum nanum are reported from South Australia. M. nanum is the commonest cause of ringworm in pigs and is known to cause porcine infections in Australia. This report shows the value of mycological investigations in determining the possible source of such infections.", "contents": "Microsporum nanum infection in South Australia. Two cases of human ringworm caused by the dermatophyte Microsporum nanum are reported from South Australia. M. nanum is the commonest cause of ringworm in pigs and is known to cause porcine infections in Australia. This report shows the value of mycological investigations in determining the possible source of such infections.", "PMID": 958065}
{"id": "pubmed23n0032_2966", "title": "Finance and fertility: the effects of heavy taxation on the birth rate.", "content": "Increasing taxation since World War II has been a factor in reducing the marriage rate and consequently the birth rate in Australia, as a result of raising the cost of living indicated by the retail price index. Taxation has increased enormously though the population has not.", "contents": "Finance and fertility: the effects of heavy taxation on the birth rate. Increasing taxation since World War II has been a factor in reducing the marriage rate and consequently the birth rate in Australia, as a result of raising the cost of living indicated by the retail price index. Taxation has increased enormously though the population has not.", "PMID": 958066}
{"id": "pubmed23n0032_2967", "title": "Methadone blockade in the treatment of opiate addiction: a follow-up study.", "content": "This paper describes methadone blockade treatment of 50 opiate addicts. At follow-up, 36 patients were traced and interviewed. Results show that 89% of the 36 patients cooperated with the treatment programme, 88% had no new criminal convictions and 75% remained drug-free, apart from taking methadone during the follow-up period (mean, 12-5 months). Five of the 36 patients were free of all drug addictions, including methadone.", "contents": "Methadone blockade in the treatment of opiate addiction: a follow-up study. This paper describes methadone blockade treatment of 50 opiate addicts. At follow-up, 36 patients were traced and interviewed. Results show that 89% of the 36 patients cooperated with the treatment programme, 88% had no new criminal convictions and 75% remained drug-free, apart from taking methadone during the follow-up period (mean, 12-5 months). Five of the 36 patients were free of all drug addictions, including methadone.", "PMID": 958067}
{"id": "pubmed23n0032_2968", "title": "Diabetes insipidus treated by DDAVP.", "content": "The symptomatic treatment of diabetes insipidus with pitressin is well known and very effective. The only problem concerns the type of pitressin to be used. In the past, pitressin snuff and various other intranasal instillations have been used, but these either have not been very effective, or have resulted in unpleasant side effects such as rhinitis. Until recently, the only effective form of the drug available in Australia has been pitressin tannate in oil. This often has to be given as a daily injection, which, like all intramuscular injections, is painful and, being in an oily base, is particularly likely to result in abscess formation. The recent introduction of 1-deamino-8-D-arginine-vasopressin (DDAVP), which can be simply instilled into the nostril, appears to present an advance in therapy.", "contents": "Diabetes insipidus treated by DDAVP. The symptomatic treatment of diabetes insipidus with pitressin is well known and very effective. The only problem concerns the type of pitressin to be used. In the past, pitressin snuff and various other intranasal instillations have been used, but these either have not been very effective, or have resulted in unpleasant side effects such as rhinitis. Until recently, the only effective form of the drug available in Australia has been pitressin tannate in oil. This often has to be given as a daily injection, which, like all intramuscular injections, is painful and, being in an oily base, is particularly likely to result in abscess formation. The recent introduction of 1-deamino-8-D-arginine-vasopressin (DDAVP), which can be simply instilled into the nostril, appears to present an advance in therapy.", "PMID": 958068}
{"id": "pubmed23n0032_2969", "title": "A commercial weight-reducing organization: a critical analysis.", "content": "A statistical analysis of the records of a commercial weight-reducing organization shows weight loss rates higher than those achieved by non-commercial weight reduction organizations and most medical trials, with 25.9% of women losing more than 9kg and 22% reaching goal weights. A reducing diet, aided by financial incentives and supportive group pressures, is suggested as the basis for these results.", "contents": "A commercial weight-reducing organization: a critical analysis. A statistical analysis of the records of a commercial weight-reducing organization shows weight loss rates higher than those achieved by non-commercial weight reduction organizations and most medical trials, with 25.9% of women losing more than 9kg and 22% reaching goal weights. A reducing diet, aided by financial incentives and supportive group pressures, is suggested as the basis for these results.", "PMID": 958075}
{"id": "pubmed23n0032_2970", "title": "Gallbladder rupture, an isolated seat belt injury.", "content": "This report  describes a patient who suffered free intraperitoneal perforation of a previously normal gallbladder as an isolated abdominal injury from seat belt compression. It is postulated that a  prerequisite for such an injury is distension of the gallbladder with bile. Alcohol ingestion in the fasting state is likely to produce  such a situation.", "contents": "Gallbladder rupture, an isolated seat belt injury. This report  describes a patient who suffered free intraperitoneal perforation of a previously normal gallbladder as an isolated abdominal injury from seat belt compression. It is postulated that a  prerequisite for such an injury is distension of the gallbladder with bile. Alcohol ingestion in the fasting state is likely to produce  such a situation.", "PMID": 958076}
{"id": "pubmed23n0032_2971", "title": "Thrombosis-perspective on a major side effect of oral contraceptives.", "content": "Evidence from retrospective studies suggests that the risk of thrombosis is related to the dose of  oestrogen in oral contraceptives. Postoperative thrombosis is four  times more likely to occur if oral contraceptives containing oestrogen are used  before a major surgical procedure. Older women who smoke are at greater risk, especially when the surgery is performed during the winter months. It is recommended that barrier methods of contraception (condom or vaginal diaphragm) be employed during the six weeks before an elective major surgical procedure or that a progestogen-only pill be used in place of a combined or sequential oral contraceptive for this period of time.", "contents": "Thrombosis-perspective on a major side effect of oral contraceptives. Evidence from retrospective studies suggests that the risk of thrombosis is related to the dose of  oestrogen in oral contraceptives. Postoperative thrombosis is four  times more likely to occur if oral contraceptives containing oestrogen are used  before a major surgical procedure. Older women who smoke are at greater risk, especially when the surgery is performed during the winter months. It is recommended that barrier methods of contraception (condom or vaginal diaphragm) be employed during the six weeks before an elective major surgical procedure or that a progestogen-only pill be used in place of a combined or sequential oral contraceptive for this period of time.", "PMID": 958077}
{"id": "pubmed23n0032_2972", "title": "Rural Greeks and illness: an anthropologist's viewpoint.", "content": "Traditionally, the ill in Greece are supported by kin and neighbors, who often provide diagnosis, medication and the social support required by an invalid. In Australia, the Greek patient is likely to be faced with impersonal efficiency rather than the highly personalized sympathy he is used to. The absence of supporting kin and friends may combine with lack of familiarity in the Australian system to make illness in Australia an extremely anxious experience, perhaps much more anxious than it should ever be.", "contents": "Rural Greeks and illness: an anthropologist's viewpoint. Traditionally, the ill in Greece are supported by kin and neighbors, who often provide diagnosis, medication and the social support required by an invalid. In Australia, the Greek patient is likely to be faced with impersonal efficiency rather than the highly personalized sympathy he is used to. The absence of supporting kin and friends may combine with lack of familiarity in the Australian system to make illness in Australia an extremely anxious experience, perhaps much more anxious than it should ever be.", "PMID": 958078}
{"id": "pubmed23n0032_2973", "title": "Comparative results of an obesity clinic and a commercial weight-reducing organization.", "content": "Members of a commercial weight-reducing organization achieved, on average, almost double the weight loss of patients attending an obestiy clinic, where an extensive regime of drugs and other therapies was offered. It is suggested that medical expertise could be used to supplement the benefits for patients attending community groups, rather than in the individual treatment of obesity.", "contents": "Comparative results of an obesity clinic and a commercial weight-reducing organization. Members of a commercial weight-reducing organization achieved, on average, almost double the weight loss of patients attending an obestiy clinic, where an extensive regime of drugs and other therapies was offered. It is suggested that medical expertise could be used to supplement the benefits for patients attending community groups, rather than in the individual treatment of obesity.", "PMID": 958079}
{"id": "pubmed23n0032_2974", "title": "Hexachlorophene emulsions and antiseptic skin care of newborn infants.", "content": "This paper reviews 81,756 live births and 858 infant deaths occurring during the years 1959 to 1969. Sixty-three instances of central nervous system vacuolation are reported. Prematurity is shown to be a necessary prerequisite for central nervous system vacuolation to occur during routine antiseptic skin care of newborn infants with 3% hexachlorophene emulsions. Hyperbilirubinaemia is a contributory factor in such premature infants. Long-term clinical follow-up studies show that, should central nervous system vacuolation occur in premature infants, there is no resultant detriment to their immediate clinical progress and physical and neurological development. Normal newborn infants weighing more than 2,000 g do not develop such central nervous system vacuolation during routine antiseptic skin care. The writers conclude that there is no rationale for regulations to restrict the use of 3% hexachlorophene emulsions in routine antiseptic skin care of normal newborn infants and that the benefits of such use far outweigh any possible risks from central nervous system vacuolation.", "contents": "Hexachlorophene emulsions and antiseptic skin care of newborn infants. This paper reviews 81,756 live births and 858 infant deaths occurring during the years 1959 to 1969. Sixty-three instances of central nervous system vacuolation are reported. Prematurity is shown to be a necessary prerequisite for central nervous system vacuolation to occur during routine antiseptic skin care of newborn infants with 3% hexachlorophene emulsions. Hyperbilirubinaemia is a contributory factor in such premature infants. Long-term clinical follow-up studies show that, should central nervous system vacuolation occur in premature infants, there is no resultant detriment to their immediate clinical progress and physical and neurological development. Normal newborn infants weighing more than 2,000 g do not develop such central nervous system vacuolation during routine antiseptic skin care. The writers conclude that there is no rationale for regulations to restrict the use of 3% hexachlorophene emulsions in routine antiseptic skin care of normal newborn infants and that the benefits of such use far outweigh any possible risks from central nervous system vacuolation.", "PMID": 958085}
{"id": "pubmed23n0032_2975", "title": "Some problems in diagnosis and management of spinal tuberculosis.", "content": "In Victoria spinal tuberculosis is now rare in childhood. Some features of the disease in adults are described. A paravertebral abscess, although a late feature of the disease, is commonly present before a diagnosis is made. Antituberculosis drug therapy has greatly altered the pattern of healing of both the bone lesion and, in contrast to the era before chemotherapy, the healing of the abscess is no longer the dominant factor in the healing of the bone lesion. The controversy about radical surgery is discussed.", "contents": "Some problems in diagnosis and management of spinal tuberculosis. In Victoria spinal tuberculosis is now rare in childhood. Some features of the disease in adults are described. A paravertebral abscess, although a late feature of the disease, is commonly present before a diagnosis is made. Antituberculosis drug therapy has greatly altered the pattern of healing of both the bone lesion and, in contrast to the era before chemotherapy, the healing of the abscess is no longer the dominant factor in the healing of the bone lesion. The controversy about radical surgery is discussed.", "PMID": 958086}
{"id": "pubmed23n0032_2976", "title": "Causes and clinical aspects of postoperative recurrent peptic ulcer.", "content": "General practitioners as well as surgeons should be aware that there is a high recurrence rate after the \"standard\" operation (vagotomy and pyloroplasty) for duodenal ulcer. Recognition of recurrence is much delayed. The much lower recurrence rate, with similar morbidity and mortality rates, after vagotomy and antrectomy, make this the operation of choice at present. The high overall recurrence rate, the not inconsiderable operative morbidity and occasional mortality, and the high postoperative morbidity rate for peptic ulcer surgery indicate that such surgery is not to be undertaken without positive indications, such as the presence of complications. An operation undertaken solely because an ulcer recurs within, say, two years can no longer be justified.", "contents": "Causes and clinical aspects of postoperative recurrent peptic ulcer. General practitioners as well as surgeons should be aware that there is a high recurrence rate after the \"standard\" operation (vagotomy and pyloroplasty) for duodenal ulcer. Recognition of recurrence is much delayed. The much lower recurrence rate, with similar morbidity and mortality rates, after vagotomy and antrectomy, make this the operation of choice at present. The high overall recurrence rate, the not inconsiderable operative morbidity and occasional mortality, and the high postoperative morbidity rate for peptic ulcer surgery indicate that such surgery is not to be undertaken without positive indications, such as the presence of complications. An operation undertaken solely because an ulcer recurs within, say, two years can no longer be justified.", "PMID": 958088}
{"id": "pubmed23n0032_2977", "title": "[Self-inflicted injuries by injecting contaminated solutions through the chest wall (author's transl)].", "content": "In the case of three prisoners, we observed the results of their attempt to induce an infection of the pleura and lungs by injecting themselves through the chest wall with a contaminated substance in order that they might be transferred to a sanatorium. Traces of the solution they had administered, which among other substances contained faeces, were found in the chest wall, in the pleural and subphrenic area, in the lungs and in the liver. The self-inflicted injuries described, led in the case of one of the prisoners to his death following a subphrenic abscess, a necrosis and abscess of the liver, septic shock and respiratory insufficiency. The clinically recognizable results of the injection only set in after a period of latency of several days. In one case there were still traces of stool in the liver 24 days after its initial introduction.", "contents": "[Self-inflicted injuries by injecting contaminated solutions through the chest wall (author's transl)]. In the case of three prisoners, we observed the results of their attempt to induce an infection of the pleura and lungs by injecting themselves through the chest wall with a contaminated substance in order that they might be transferred to a sanatorium. Traces of the solution they had administered, which among other substances contained faeces, were found in the chest wall, in the pleural and subphrenic area, in the lungs and in the liver. The self-inflicted injuries described, led in the case of one of the prisoners to his death following a subphrenic abscess, a necrosis and abscess of the liver, septic shock and respiratory insufficiency. The clinically recognizable results of the injection only set in after a period of latency of several days. In one case there were still traces of stool in the liver 24 days after its initial introduction.", "PMID": 958095}
{"id": "pubmed23n0032_2978", "title": "[Folic acid absorption in tropical enteropathy (author's transl)].", "content": "Folic acid absoprtion was measured in 45 patients before and after folic acid treatment by means of a folate tolerance test in serum and red-blood-cell-folate. 10 healthy men recently returned from the tropics volunteered as controls. There is no significant difference in the folate-tolerance tests in serum. The difference is significant however in the red-blood-cell-folate, which is markedly lower with the patients before treatment and raises after folate-treatment to the normal value of the control group. As the transport of folic acid in the mucosa follows two steps it is taken that the disturbance is localized in the hydrolysis of polyglutamate to monoglutamate-folic acid.", "contents": "[Folic acid absorption in tropical enteropathy (author's transl)]. Folic acid absoprtion was measured in 45 patients before and after folic acid treatment by means of a folate tolerance test in serum and red-blood-cell-folate. 10 healthy men recently returned from the tropics volunteered as controls. There is no significant difference in the folate-tolerance tests in serum. The difference is significant however in the red-blood-cell-folate, which is markedly lower with the patients before treatment and raises after folate-treatment to the normal value of the control group. As the transport of folic acid in the mucosa follows two steps it is taken that the disturbance is localized in the hydrolysis of polyglutamate to monoglutamate-folic acid.", "PMID": 958097}
{"id": "pubmed23n0032_2979", "title": "[Haemolytic anaemia and acute renal failure associated with cephalothin and furosemide therapy (author's transl)].", "content": "A case report is presented of a 79 year old patient with a bilateral brochopneumonia. Four days after beginning the therapy with cephalothin and furosemide an acute renal failure appeared and six days later a haemolytic anaemia was observed. The modificated Coombs test was positive. The post mortem histiological examination of the kidneys revealed cloudy swelling, necrosis of tubuli, containing protein cylinders (\"chromoproteid kidney\").", "contents": "[Haemolytic anaemia and acute renal failure associated with cephalothin and furosemide therapy (author's transl)]. A case report is presented of a 79 year old patient with a bilateral brochopneumonia. Four days after beginning the therapy with cephalothin and furosemide an acute renal failure appeared and six days later a haemolytic anaemia was observed. The modificated Coombs test was positive. The post mortem histiological examination of the kidneys revealed cloudy swelling, necrosis of tubuli, containing protein cylinders (\"chromoproteid kidney\").", "PMID": 958099}
{"id": "pubmed23n0032_2980", "title": "[Hemorrhage caused by thrombocytopathies: new aspect of treatment (author's transl)].", "content": "Hemorrhage due to thrombocytopathies can in most cases be stopped by application of the homologous coagulation-active phospholipide complex Fibraccel, as shown in a study in which 78 patients suffering from various thrombocytopathies were checked. This effect is especially important in patients with immunothrombocytopathies which do not permit any thrombocyte substitution. Recalcification time and thromboelastogram should be normalised during Fibraccel treatment. In existing extravascular coagulation Fibraccel treatment is contraindicated, especially in cases of inadequate protection by heparin.", "contents": "[Hemorrhage caused by thrombocytopathies: new aspect of treatment (author's transl)]. Hemorrhage due to thrombocytopathies can in most cases be stopped by application of the homologous coagulation-active phospholipide complex Fibraccel, as shown in a study in which 78 patients suffering from various thrombocytopathies were checked. This effect is especially important in patients with immunothrombocytopathies which do not permit any thrombocyte substitution. Recalcification time and thromboelastogram should be normalised during Fibraccel treatment. In existing extravascular coagulation Fibraccel treatment is contraindicated, especially in cases of inadequate protection by heparin.", "PMID": 958100}
{"id": "pubmed23n0032_2981", "title": "[Consumption coagulopathy in a case of cystic spleen (author's transl)].", "content": "A case of a 65-year old female patient is reported, dealing with consumption coagulopathy and secondary hyperfibrinolysis due to cystic spleen. Following treatment with heparin, antifibrinolytic agents and replacement of clotting factors prior to surgery, the patient attained a satisfactory condition to be operated on. After removal of the cystic spleen, which was interspersed with massive hemorrhages, blood coagulation returned to normal quickly and continuously. The pathogenesis of consumption coagulopathy in relation to cystic spleen is discussed and it is referred to the pathomechanism of similar blood disorders in hemangiomatous and cavernous lesions of Kasabach-Merritt-syndrome, Klippel-Trenaunay-syndrome and pachymeningeosis haemorrhagica interna.", "contents": "[Consumption coagulopathy in a case of cystic spleen (author's transl)]. A case of a 65-year old female patient is reported, dealing with consumption coagulopathy and secondary hyperfibrinolysis due to cystic spleen. Following treatment with heparin, antifibrinolytic agents and replacement of clotting factors prior to surgery, the patient attained a satisfactory condition to be operated on. After removal of the cystic spleen, which was interspersed with massive hemorrhages, blood coagulation returned to normal quickly and continuously. The pathogenesis of consumption coagulopathy in relation to cystic spleen is discussed and it is referred to the pathomechanism of similar blood disorders in hemangiomatous and cavernous lesions of Kasabach-Merritt-syndrome, Klippel-Trenaunay-syndrome and pachymeningeosis haemorrhagica interna.", "PMID": 958101}
{"id": "pubmed23n0032_2982", "title": "[Investigations of the mode action of buformin using a 3H-labelled glucose in people with a healthy metabolism (author's transl)].", "content": "In a total of 13 volunteers the changes in carbohydrate metabolism was investigated after administration of prednisolone, of buformin, and of a combination of prednisolone and buformin using 3H-labelled glucose. Under steady-state-conditions the glucose-turnover was estimated. Although, the changes of glucose assimilation of labelled and unlabelled glucose was observed to see the magnitude of glucose utilisation and of gluconeogenesis. It was concluded that biguanides enhance the glucose utilisation. Depending on the glucose level at the same time the gluconeogenesis is stimulated or inhibited.", "contents": "[Investigations of the mode action of buformin using a 3H-labelled glucose in people with a healthy metabolism (author's transl)]. In a total of 13 volunteers the changes in carbohydrate metabolism was investigated after administration of prednisolone, of buformin, and of a combination of prednisolone and buformin using 3H-labelled glucose. Under steady-state-conditions the glucose-turnover was estimated. Although, the changes of glucose assimilation of labelled and unlabelled glucose was observed to see the magnitude of glucose utilisation and of gluconeogenesis. It was concluded that biguanides enhance the glucose utilisation. Depending on the glucose level at the same time the gluconeogenesis is stimulated or inhibited.", "PMID": 958105}
{"id": "pubmed23n0032_2983", "title": "[Lactic acidosis and biguanid therapy (author's transl)].", "content": "Ten case histories of patients with lactic acidosis and biguanide therapy are presented. 6 patients received phenformin, 4 buformin. The symptomatology was characterized by somnolence or unconsciousness with hyperventilation, renal insufficiency, signs of infection occasionally with detection of gram negative rods and in later stages circulatory insufficiency with high central venous pressure. Glucose, insulin, bicarbonate, dialysis, antibiotics and katecholamines were the therapeutic measurements. It is the proposal of this communication to call attention again to the potential toxicity of biguanids which makes necessary the strict observation of contraindications.", "contents": "[Lactic acidosis and biguanid therapy (author's transl)]. Ten case histories of patients with lactic acidosis and biguanide therapy are presented. 6 patients received phenformin, 4 buformin. The symptomatology was characterized by somnolence or unconsciousness with hyperventilation, renal insufficiency, signs of infection occasionally with detection of gram negative rods and in later stages circulatory insufficiency with high central venous pressure. Glucose, insulin, bicarbonate, dialysis, antibiotics and katecholamines were the therapeutic measurements. It is the proposal of this communication to call attention again to the potential toxicity of biguanids which makes necessary the strict observation of contraindications.", "PMID": 958106}
{"id": "pubmed23n0032_2984", "title": "[Metabolism of phenylbutazone in the liver (author's transl)].", "content": "The object of the investigation was to study the capacity of the liver to metabolize drugs under diversified conditions (cirrhosis, hepatitis, cholestasis in alcoholics, in diabetics as well as in eplieptics treated with barbiturates) as well as the possibility of inducing this function. To accomplish this we determined the half-life of Butazolidin. Only in cirrhotics it was shown that the capacity to metabolize Butazolidin was reduced. Induction with barbiturates increased hepatic captation of the drug but did not modify the biotransformation of the same. This is attributed to the development of a \"hypoactive hypertrophy\" of the reticuloendothelium.", "contents": "[Metabolism of phenylbutazone in the liver (author's transl)]. The object of the investigation was to study the capacity of the liver to metabolize drugs under diversified conditions (cirrhosis, hepatitis, cholestasis in alcoholics, in diabetics as well as in eplieptics treated with barbiturates) as well as the possibility of inducing this function. To accomplish this we determined the half-life of Butazolidin. Only in cirrhotics it was shown that the capacity to metabolize Butazolidin was reduced. Induction with barbiturates increased hepatic captation of the drug but did not modify the biotransformation of the same. This is attributed to the development of a \"hypoactive hypertrophy\" of the reticuloendothelium.", "PMID": 958107}
{"id": "pubmed23n0032_2985", "title": "[Studies on calcium absorption out of a calcium-vitamin-D-preparation using calcium-47 (author's transl)].", "content": "Calcium absorption in man was studied by means of labelling an ampoule of Frubiase Calcium forte with calcium-47. For comparative purpose a solution containing the same amount of calcium salts as the ampoule was labelled as well. Already 10 minutes after oral administration of Ca-47 in 10 ml of Frubiase Calcium forte radioactivity was statistically significant measurable in blood. At the same time there was no significant radioactivity demonstrable out of the calcium salt solution. Between 45 and 120 minutes following oral application of the isotope both groups showed a statistically significant difference concerning the measurable blood activity. The Frubiase Calcium forte values were always higher, especially one and two hours after administration of the radiocalcium (up to 40%). The levels at these times correlate well with the total calcium absorption. Measurements over the compact bone region of the ankle demonstrated significantly higher values of calcium-47 in case of Frubiase Calcium forte compared with the calcium salt solution. These findings indicate in the case of Frubiase Calcium forte a higher bone mineral accretion. There is a good direct proportionality between the amount of these values and the total calcium absorption.", "contents": "[Studies on calcium absorption out of a calcium-vitamin-D-preparation using calcium-47 (author's transl)]. Calcium absorption in man was studied by means of labelling an ampoule of Frubiase Calcium forte with calcium-47. For comparative purpose a solution containing the same amount of calcium salts as the ampoule was labelled as well. Already 10 minutes after oral administration of Ca-47 in 10 ml of Frubiase Calcium forte radioactivity was statistically significant measurable in blood. At the same time there was no significant radioactivity demonstrable out of the calcium salt solution. Between 45 and 120 minutes following oral application of the isotope both groups showed a statistically significant difference concerning the measurable blood activity. The Frubiase Calcium forte values were always higher, especially one and two hours after administration of the radiocalcium (up to 40%). The levels at these times correlate well with the total calcium absorption. Measurements over the compact bone region of the ankle demonstrated significantly higher values of calcium-47 in case of Frubiase Calcium forte compared with the calcium salt solution. These findings indicate in the case of Frubiase Calcium forte a higher bone mineral accretion. There is a good direct proportionality between the amount of these values and the total calcium absorption.", "PMID": 958108}
{"id": "pubmed23n0032_2986", "title": "[Body potassium and skinfold thickness in an isocaloric, fat-rich diet (author's transl)].", "content": "Nine partly weak overweighted sport students got a fat rich isocaloric diet (70% fat, 20% protein, 10% carbohydrates) for three weeks. Body weight, body potassium and skinfold thicknesses were measured. A constant loss of body weight was the result. Lean body mass and body fat decreased. It is supposed that protein is used for gluconeogenesis if carbohydrate reduction is nearly total.", "contents": "[Body potassium and skinfold thickness in an isocaloric, fat-rich diet (author's transl)]. Nine partly weak overweighted sport students got a fat rich isocaloric diet (70% fat, 20% protein, 10% carbohydrates) for three weeks. Body weight, body potassium and skinfold thicknesses were measured. A constant loss of body weight was the result. Lean body mass and body fat decreased. It is supposed that protein is used for gluconeogenesis if carbohydrate reduction is nearly total.", "PMID": 958109}
{"id": "pubmed23n0032_2987", "title": "[Sudden, unexpected death of infants and electromagnetic fields (author's transl)].", "content": "The examination of 337 cases of Sudden Infant Death Syndorme (SIDS) ro Crib Deaths in Philadelphia, Penn., USA, and 294 cases in Hamburg, Federal Republic of Germany, shows regional concentrations which are close to uncommon magnetic fields or stray electric currents in the ground. The risk in the cellar and first floor of houses is higher than in the other floors. Therefore a possible causal relationship between electromagnetic fields and SIDS cases must be considered and checked by on-site measurements, animal experiments, and by more research on magnetic fields as stressor for infants.", "contents": "[Sudden, unexpected death of infants and electromagnetic fields (author's transl)]. The examination of 337 cases of Sudden Infant Death Syndorme (SIDS) ro Crib Deaths in Philadelphia, Penn., USA, and 294 cases in Hamburg, Federal Republic of Germany, shows regional concentrations which are close to uncommon magnetic fields or stray electric currents in the ground. The risk in the cellar and first floor of houses is higher than in the other floors. Therefore a possible causal relationship between electromagnetic fields and SIDS cases must be considered and checked by on-site measurements, animal experiments, and by more research on magnetic fields as stressor for infants.", "PMID": 958113}
{"id": "pubmed23n0032_2988", "title": "Neutrophil leukocyte reserves in lymphoreticular malignancies.", "content": "The marginal neutrophil leukocyte pool and the bone marrow neutrophil reserves were assessed in 43 patients with lymphoreticular malignancies, using epinephrine and steroid stimulation. Thirty-three patients were studied before staging, following splenectomy, before and after nodal irradiation and during remission induction chemotherapy. Ten patients in partial remission were studied after 12 month or more of chemotherapy. A neutrophil count of 1,500-2,500 cells/mm3 is not a sufficient guide for midifying the chemotherapy. The response to steroid stimulation may be a more dependable guide to evaluate the patient's potential risk for infection.", "contents": "Neutrophil leukocyte reserves in lymphoreticular malignancies. The marginal neutrophil leukocyte pool and the bone marrow neutrophil reserves were assessed in 43 patients with lymphoreticular malignancies, using epinephrine and steroid stimulation. Thirty-three patients were studied before staging, following splenectomy, before and after nodal irradiation and during remission induction chemotherapy. Ten patients in partial remission were studied after 12 month or more of chemotherapy. A neutrophil count of 1,500-2,500 cells/mm3 is not a sufficient guide for midifying the chemotherapy. The response to steroid stimulation may be a more dependable guide to evaluate the patient's potential risk for infection.", "PMID": 958159}
{"id": "pubmed23n0032_2989", "title": "Combination chemotherapy with adramycin (NSC-123127) and dimethyl triazeno imidazole carboxamide (DTIC) (NSC-45388) in children with metastatic solid tumors.", "content": "Combination chemotherapy with adriamycin and DTIC was used in 102 evaluable patients under 15 years of age who had previously treated metastatic solid tumors. Responses, defined as 50% or more reduction in all tumor masses, occurred in 10 out of 27 patients with neuroblastoma, 3 out of 8 patients with Wilms tumor, 7 out 15 patients with Ewing sarcoma, 2 out of 6 patients with osteosarcoma, 5 out of 13 patients with rhabdomyosarcoma, and 15 out of 33 patients with miscellaneous tumors which included a patient who had a complete regression of an extensive juvenile angiofibroma. Response rate to combination chemotherapy with adriamycin and DTIC in patients with Ewing sarcoma was significantly superior to the response rate obtained with adriamycin alone in another Southwest Oncology Group Study. Major toxicity included nausea, vomiting, myelosuppression, high incidence of pneumocystis carinii pneumonia (5 patients) and congestive heart failure (4 patients). There was 7 drug-associated deaths due to sepsis (1), pneumocystis carinii pneumonia (4), and congestive heart failure (2).", "contents": "Combination chemotherapy with adramycin (NSC-123127) and dimethyl triazeno imidazole carboxamide (DTIC) (NSC-45388) in children with metastatic solid tumors. Combination chemotherapy with adriamycin and DTIC was used in 102 evaluable patients under 15 years of age who had previously treated metastatic solid tumors. Responses, defined as 50% or more reduction in all tumor masses, occurred in 10 out of 27 patients with neuroblastoma, 3 out of 8 patients with Wilms tumor, 7 out 15 patients with Ewing sarcoma, 2 out of 6 patients with osteosarcoma, 5 out of 13 patients with rhabdomyosarcoma, and 15 out of 33 patients with miscellaneous tumors which included a patient who had a complete regression of an extensive juvenile angiofibroma. Response rate to combination chemotherapy with adriamycin and DTIC in patients with Ewing sarcoma was significantly superior to the response rate obtained with adriamycin alone in another Southwest Oncology Group Study. Major toxicity included nausea, vomiting, myelosuppression, high incidence of pneumocystis carinii pneumonia (5 patients) and congestive heart failure (4 patients). There was 7 drug-associated deaths due to sepsis (1), pneumocystis carinii pneumonia (4), and congestive heart failure (2).", "PMID": 958160}
{"id": "pubmed23n0032_2990", "title": "Clinical studies of beta-thioguanine deoxyriboside alone and in combination with arabinosyl cytosine.", "content": "Beta-thioguanine deoxyriboside (betaTGdR) is a purine nucleoside derivative which was studied alone or in combination with arabinosyl cytosine (Ara-C) in patients with solid tumors and acute leukemia. No significant responses were observed in 22 patients with solid tumors. The response rate with betaTGdR alone in acute leukemia was 26% and in combination with Ara-C was 24%. Responses were generally of short duration. Toxicity included myelosuppression, nausea, stomatitis, hyperpigmentation, photosensitivity, and liver function abnormalities.", "contents": "Clinical studies of beta-thioguanine deoxyriboside alone and in combination with arabinosyl cytosine. Beta-thioguanine deoxyriboside (betaTGdR) is a purine nucleoside derivative which was studied alone or in combination with arabinosyl cytosine (Ara-C) in patients with solid tumors and acute leukemia. No significant responses were observed in 22 patients with solid tumors. The response rate with betaTGdR alone in acute leukemia was 26% and in combination with Ara-C was 24%. Responses were generally of short duration. Toxicity included myelosuppression, nausea, stomatitis, hyperpigmentation, photosensitivity, and liver function abnormalities.", "PMID": 958161}
{"id": "pubmed23n0032_2991", "title": "Phase II study of profiromycin vs mitomycin-C utilizing acute intermittent schedules.", "content": "A randomized prosective study of Mitomycin-C and its N-methyl derivative, Porfiromycin, was conducted. Thirty-two patients with disseminated gastrointestinal cancer or other disseminated abdominal adenocarcinoma were treated with Mitomycin-C; 31 patients received Porfiromycin. Both drugs were given by acute intermittent bolus schedule (Mitomucin-C , 22.5 mg/M2 or Porfiromycin, 75 mg/M2 every 6--8 weeks as a single bolus i.v. injection). Eleven patients (34%) who received Mitomycin-C entered into partial remission. In 10 of the 31 patients (32%) receiving Porfiromycin, partial remission occured. Analysis by tumor type demonstrated that in the Mitomycin-C treated group responses occured in 4 of 12 patients with colorectal carcinoma, in 4 of 9 with upper GI cancers, and in 3 of 11 with ovarian cancer. Correspondingly in Porfiromycin group responses occured in 2 of 12 colorectal carcinoma patients, in 3 of 7 upper GI cancer patients, and in 5 of 12 ovarian cancer patients. Both drugs produced significant myelosuppression; however, Porfiromycin toxicity appeared more cumulative. Further clinical trial of Mitomycin in an acute intermittent bolus schedule appears justified.", "contents": "Phase II study of profiromycin vs mitomycin-C utilizing acute intermittent schedules. A randomized prosective study of Mitomycin-C and its N-methyl derivative, Porfiromycin, was conducted. Thirty-two patients with disseminated gastrointestinal cancer or other disseminated abdominal adenocarcinoma were treated with Mitomycin-C; 31 patients received Porfiromycin. Both drugs were given by acute intermittent bolus schedule (Mitomucin-C , 22.5 mg/M2 or Porfiromycin, 75 mg/M2 every 6--8 weeks as a single bolus i.v. injection). Eleven patients (34%) who received Mitomycin-C entered into partial remission. In 10 of the 31 patients (32%) receiving Porfiromycin, partial remission occured. Analysis by tumor type demonstrated that in the Mitomycin-C treated group responses occured in 4 of 12 patients with colorectal carcinoma, in 4 of 9 with upper GI cancers, and in 3 of 11 with ovarian cancer. Correspondingly in Porfiromycin group responses occured in 2 of 12 colorectal carcinoma patients, in 3 of 7 upper GI cancer patients, and in 5 of 12 ovarian cancer patients. Both drugs produced significant myelosuppression; however, Porfiromycin toxicity appeared more cumulative. Further clinical trial of Mitomycin in an acute intermittent bolus schedule appears justified.", "PMID": 958162}
{"id": "pubmed23n0032_2992", "title": "In vivo quantitation of lesion radioactivity using external counting methods.", "content": "Quantitative external counting methods for the in vivo determination of the activity uniformly distributed in an area of interest (lesion) have been developed. The theoretical work has been extended to include the effects of regions of differing attenuation coefficient and of non-target organ activity in the tissue surrounding the lesion. A clinical quantitative procedure utilizing a \"calibrated\" gamma camera and associated computer system is described. Studies including a bone substitute material in a water phantom confirmed the basic theoretical work.", "contents": "In vivo quantitation of lesion radioactivity using external counting methods. Quantitative external counting methods for the in vivo determination of the activity uniformly distributed in an area of interest (lesion) have been developed. The theoretical work has been extended to include the effects of regions of differing attenuation coefficient and of non-target organ activity in the tissue surrounding the lesion. A clinical quantitative procedure utilizing a \"calibrated\" gamma camera and associated computer system is described. Studies including a bone substitute material in a water phantom confirmed the basic theoretical work.", "PMID": 958163}
{"id": "pubmed23n0032_2993", "title": "Calculations of two-dimensional electric field and potential distributions aroun electrodes in conducting media.", "content": "Tumor cells can be killed by heating to 42.5degreesC. It is convenient to localize heating by passing electric current at 500 kHz through tissue between a pair of electrodes. It is desirable to determine, for a given electrode-tissue configuration, what the resulting field and, therefore, current distribution will be. A computer program FFEARS was used to calculate electric field and potential distributions in two dimensions for three simple cases.", "contents": "Calculations of two-dimensional electric field and potential distributions aroun electrodes in conducting media. Tumor cells can be killed by heating to 42.5degreesC. It is convenient to localize heating by passing electric current at 500 kHz through tissue between a pair of electrodes. It is desirable to determine, for a given electrode-tissue configuration, what the resulting field and, therefore, current distribution will be. A computer program FFEARS was used to calculate electric field and potential distributions in two dimensions for three simple cases.", "PMID": 958164}
{"id": "pubmed23n0032_2994", "title": "Method for calculating dose when lung tissue lies in the treatment field.", "content": "The absorbed dose in lung and beyond lung as a result of increased lung transmission of x and gamma irradiation is described. The correction factor used to calculate the absorbed dose is a function of beam energy, field area, lung density, and lung and soft tissue depth. Agreement between measurements and calculations in the Alderson phantom is within 3%. An example of how this technique can be used is described.", "contents": "Method for calculating dose when lung tissue lies in the treatment field. The absorbed dose in lung and beyond lung as a result of increased lung transmission of x and gamma irradiation is described. The correction factor used to calculate the absorbed dose is a function of beam energy, field area, lung density, and lung and soft tissue depth. Agreement between measurements and calculations in the Alderson phantom is within 3%. An example of how this technique can be used is described.", "PMID": 958166}
{"id": "pubmed23n0032_2995", "title": "Phase effects in diagnostic radiological images.", "content": "The effect of phase on the quality of diagnostic radiological images is investigated on a theoretical basis and with the use of the computer simulations. Phase effects are divided into two categories: linear phase shift and asymmetric phase shift. Using a model of the x-ray focal spot distribution composed of two Gaussian functions, it is shown that the phase transfer function (PTF) is generally a continuous nonlinear function of spatial frequency. The linear phase shift serves only to shift the location of the image. The asymmetric phase shift in the model studied tends to accompany an improvement in the modulation transfer function (MTF) in the midfrequency region while introducing \"wows\" into the image. Contrast reversals are shown to be inherent in line spread functions (LSFs) which do not necessarily include linear or asymmetric phase effects, i.e., they are the result of another phenomenon.", "contents": "Phase effects in diagnostic radiological images. The effect of phase on the quality of diagnostic radiological images is investigated on a theoretical basis and with the use of the computer simulations. Phase effects are divided into two categories: linear phase shift and asymmetric phase shift. Using a model of the x-ray focal spot distribution composed of two Gaussian functions, it is shown that the phase transfer function (PTF) is generally a continuous nonlinear function of spatial frequency. The linear phase shift serves only to shift the location of the image. The asymmetric phase shift in the model studied tends to accompany an improvement in the modulation transfer function (MTF) in the midfrequency region while introducing \"wows\" into the image. Contrast reversals are shown to be inherent in line spread functions (LSFs) which do not necessarily include linear or asymmetric phase effects, i.e., they are the result of another phenomenon.", "PMID": 958165}
{"id": "pubmed23n0032_2996", "title": "Intensity distribution, modulation transfer function, and the effective dimension of a line-focus x-ray focal spot.", "content": "The radiant intensity along the anode-cathode dimension of a line-focus x-ray focal spot tends to peak at the center and decrease gradually to zero at the edges. In recognition of this varying intensity, the ICRU has recommended that the anode-cathode dimension of the focal spot be reported as 0.7 times the dimension measured with a pinhole camera. In this paper more objective correction factors are developed. These correction factors are based upon comparison of calculated MTFs of focal spots with Gaussian and cosine intensity distributions with the MTF for focal spots with a uniform intensity distribution.", "contents": "Intensity distribution, modulation transfer function, and the effective dimension of a line-focus x-ray focal spot. The radiant intensity along the anode-cathode dimension of a line-focus x-ray focal spot tends to peak at the center and decrease gradually to zero at the edges. In recognition of this varying intensity, the ICRU has recommended that the anode-cathode dimension of the focal spot be reported as 0.7 times the dimension measured with a pinhole camera. In this paper more objective correction factors are developed. These correction factors are based upon comparison of calculated MTFs of focal spots with Gaussian and cosine intensity distributions with the MTF for focal spots with a uniform intensity distribution.", "PMID": 958167}
{"id": "pubmed23n0032_2997", "title": "Calculated neutron dose rates and flux densities from implantable californium-252 point and line sources.", "content": "The results of neutron-transport flux-density and dose rate calculations for implantable Californium-252 point and line sources in essentially infinite tissue-equivalent material are presented. The point-source flux densities were obtained from a discrete ordinates calculation, and the point dose rates were established by multiplying the flux densities by their appropriate kerma factors. Line-source dose rates were evaluated by integrating the point dose rates over the length of the line source. Dose-rate data are given within a 20 X 20-cm region from the source center for source lengths of 1.5, 2, and 3 cm. The dose rates established by these calculations showed good agreement with an independent Monte Carlo calculation. Detailed point-source flux-density data as a function of energy and position are also given.", "contents": "Calculated neutron dose rates and flux densities from implantable californium-252 point and line sources. The results of neutron-transport flux-density and dose rate calculations for implantable Californium-252 point and line sources in essentially infinite tissue-equivalent material are presented. The point-source flux densities were obtained from a discrete ordinates calculation, and the point dose rates were established by multiplying the flux densities by their appropriate kerma factors. Line-source dose rates were evaluated by integrating the point dose rates over the length of the line source. Dose-rate data are given within a 20 X 20-cm region from the source center for source lengths of 1.5, 2, and 3 cm. The dose rates established by these calculations showed good agreement with an independent Monte Carlo calculation. Detailed point-source flux-density data as a function of energy and position are also given.", "PMID": 958169}
{"id": "pubmed23n0032_2998", "title": "Real-time display of radiation field intensity distribution.", "content": "A real-time display system has been designed and tested for use with a radiation field monitor. The system uses only pulse and waveform generators, a multiplexer, and an oscilloscope. The display consists of either a three-dimensional representation of the field intensity distribution or a series of simultaneous profiles.", "contents": "Real-time display of radiation field intensity distribution. A real-time display system has been designed and tested for use with a radiation field monitor. The system uses only pulse and waveform generators, a multiplexer, and an oscilloscope. The display consists of either a three-dimensional representation of the field intensity distribution or a series of simultaneous profiles.", "PMID": 958171}
{"id": "pubmed23n0032_2999", "title": "Mathematical model of conventional tomography.", "content": "A Fourier decomposition approach is used to study the imaging properties of conventional tomography. Spatial frequency response curves (MTFs) are calculated for both linear and axial transverse tomography. These curves depend on the product of the spatial frequency of the sinusoidal density variations in thin layers parallel to the tomographic plane and the distance between such layers and the tomographic plane. Based on the spatial frequency response curves, a quantitative definition of tomographic layer thickness is given. Furthermore, the spatial frequency response curves suggest that unattenuated low-frequency information from outside the tomographic layer limits the resolution in conventional tomograms and that high-pass spatial filtering of the image may substantially improve the diagnostic quality of tomographic images, particularly in the identification of boundaries.", "contents": "Mathematical model of conventional tomography. A Fourier decomposition approach is used to study the imaging properties of conventional tomography. Spatial frequency response curves (MTFs) are calculated for both linear and axial transverse tomography. These curves depend on the product of the spatial frequency of the sinusoidal density variations in thin layers parallel to the tomographic plane and the distance between such layers and the tomographic plane. Based on the spatial frequency response curves, a quantitative definition of tomographic layer thickness is given. Furthermore, the spatial frequency response curves suggest that unattenuated low-frequency information from outside the tomographic layer limits the resolution in conventional tomograms and that high-pass spatial filtering of the image may substantially improve the diagnostic quality of tomographic images, particularly in the identification of boundaries.", "PMID": 958168}
{"id": "pubmed23n0032_3000", "title": "Calculations of two-dimensional electric field and potential distributions around electrodes in conducting media.", "content": "Tumor cells can be killed by heating to 42.5 degrees C. It is convenient to localize heating by passing electric current at 500 kHz through tissue between a pair of electrodes. It is desirable to determine, for a given electrode-tissue configuration, what the resulting field and, therefore, current distribution will be. A computer program FFEARS was used to calculate electric field and potential distributions in two dimensions for three simple cases.", "contents": "Calculations of two-dimensional electric field and potential distributions around electrodes in conducting media. Tumor cells can be killed by heating to 42.5 degrees C. It is convenient to localize heating by passing electric current at 500 kHz through tissue between a pair of electrodes. It is desirable to determine, for a given electrode-tissue configuration, what the resulting field and, therefore, current distribution will be. A computer program FFEARS was used to calculate electric field and potential distributions in two dimensions for three simple cases.", "PMID": 958170}
{"id": "pubmed23n0032_3001", "title": "Genetic exchanges caused by ultraviolet photoproducts in phage lambda DNA molecules: the role of DNA replication.", "content": "Genetic recombination induced by structural damage in DNA molecules was investigated in E. coli K12 (lambda) lysogens infected with genetically marked phage lambda. Photoproducts were induced in the phage DNA before infection by exposing them either to 313 nm light in the presence of acetophenone or to 254 nm light. To test the role of the replication of the damaged phage DNA on the frequency of the induced recombination, both heteroimmune and homimmune crosses were performed. First, samples of a heteroimmune phage lambda imm434 P80 exposed to these treatments were allowed to infect cells lysogenic for prophage lambda cI857 P3. Phage DNA replication and maturation took place, and the resulting progeny phages were assayed for the frequency of P+ recombinants. Recombination was less frequent in infected cells exposed to visible light and in wild type cells able to perform excision repair than in excision-defective lysogens. Therefore, much of the induced recombination can be attributed to the pyrimidine dimers in the phage DNA, the only photoproducts known to be dissociated by photoreactivating enzyme. Second, in homoimmune crosses, samples of similarly treated homoimmune lambda P3 phages were allowed to infect lysogens carrying lambda cI857 P80. Replication of the phage DNA containing ultraviolet photoproducts was repressed by lambda immunity, and was further blocked by the lack of the P gene product needed for replication. The lysogens were purified and scored for both colony forming ability and for P+ recombinant prophages. The 254 nm photoproducts increased the frequency of recombination in these homimmune crosses, even though phage DNA replication was blocked. Irradiation with 313 nm light and acetophenone M, which produces dimers and unknown photoproducts, was not as effective per dimer as the 254 nm light. It is concluded from these results that certain unidentified 254 nm photoproducts can cause recombination even in the absence of DNA replication. They are not pyrimidine dimers, as they are not susceptible to excision repair or photoreactivation. In contrast, pyrimidine dimers appear to cause recombination only when the DNA containing them undergoes replication.", "contents": "Genetic exchanges caused by ultraviolet photoproducts in phage lambda DNA molecules: the role of DNA replication. Genetic recombination induced by structural damage in DNA molecules was investigated in E. coli K12 (lambda) lysogens infected with genetically marked phage lambda. Photoproducts were induced in the phage DNA before infection by exposing them either to 313 nm light in the presence of acetophenone or to 254 nm light. To test the role of the replication of the damaged phage DNA on the frequency of the induced recombination, both heteroimmune and homimmune crosses were performed. First, samples of a heteroimmune phage lambda imm434 P80 exposed to these treatments were allowed to infect cells lysogenic for prophage lambda cI857 P3. Phage DNA replication and maturation took place, and the resulting progeny phages were assayed for the frequency of P+ recombinants. Recombination was less frequent in infected cells exposed to visible light and in wild type cells able to perform excision repair than in excision-defective lysogens. Therefore, much of the induced recombination can be attributed to the pyrimidine dimers in the phage DNA, the only photoproducts known to be dissociated by photoreactivating enzyme. Second, in homoimmune crosses, samples of similarly treated homoimmune lambda P3 phages were allowed to infect lysogens carrying lambda cI857 P80. Replication of the phage DNA containing ultraviolet photoproducts was repressed by lambda immunity, and was further blocked by the lack of the P gene product needed for replication. The lysogens were purified and scored for both colony forming ability and for P+ recombinant prophages. The 254 nm photoproducts increased the frequency of recombination in these homimmune crosses, even though phage DNA replication was blocked. Irradiation with 313 nm light and acetophenone M, which produces dimers and unknown photoproducts, was not as effective per dimer as the 254 nm light. It is concluded from these results that certain unidentified 254 nm photoproducts can cause recombination even in the absence of DNA replication. They are not pyrimidine dimers, as they are not susceptible to excision repair or photoreactivation. In contrast, pyrimidine dimers appear to cause recombination only when the DNA containing them undergoes replication.", "PMID": 958200}
{"id": "pubmed23n0032_3002", "title": "Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe.", "content": "Twenty seven recessive temperature sensitive mutants have been isolated in Schizosaccharomyces pombe which are unable to complete the cell division cycle at the restrictive temperature. These mutants define 14 unlinked genes which are involved in DNA synthesis, nuclear division and cell plate formation. The products from most of these genes complete their function just before the cell cycle event in which they are involved. Physiological characterisation of the mutants has shown that DNA synthesis and nuclear division form a cycle of mutually dependent events which can operate in the absence of cell plate formation. Cell plate formation itself is usually dependent upon the completion of nuclear division.", "contents": "Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Twenty seven recessive temperature sensitive mutants have been isolated in Schizosaccharomyces pombe which are unable to complete the cell division cycle at the restrictive temperature. These mutants define 14 unlinked genes which are involved in DNA synthesis, nuclear division and cell plate formation. The products from most of these genes complete their function just before the cell cycle event in which they are involved. Physiological characterisation of the mutants has shown that DNA synthesis and nuclear division form a cycle of mutually dependent events which can operate in the absence of cell plate formation. Cell plate formation itself is usually dependent upon the completion of nuclear division.", "PMID": 958201}
{"id": "pubmed23n0032_3003", "title": "Arg-7 mutant X wild-type crosses in Chlamydomonas reinhardi: study of the enzyme produced in diploid strains.", "content": "The arg-7 locus is the structural gene for the argininosuccinate lyase (ASL). Interallelic complementation was previously found to occur between several mutants of the locus: this is indicative for the homomultimeric nature of ASL. Two complementing (arg-7-5 and arg-7-7) and two non-complementing (arg-7-1 and arg-7-6) mutants of the arg-7 locus were crossed to the pab-2 strain (which is wild-type for the arg-7 locus). In each cross, heterozygote phenotypically wild-type strains were isolated; their diploid pattern was demonstrated by various criteria: mating type, cell volume, nuclear size. The four heterozygotes were compared to the haploid wild-type and in some experiments, to the diploid strain arg-1 X pab-2 homozygous for the arg-7 locus. No difference was found in growth rate and in the Michaelis constant values for ASL. The specific activity of the enzyme produced in the heterozygotes was about 50 percent of the activity found in haploid or diploid wild-type. The heat sensitivity of ASL was also investigated in the different strains: two (containing the complementing mutations arg-7-5 and arg-7-7) of the four heterozygotes produce ASL varieties different from the wild-type enzyme as far as the thermolability is concerned. These results suggest that hybrid ASL can be formed by interaction between the products of wild-type and mutant genes. A clear dominance of the wild-type allele is expected only when the mutant allele has no product of the gene: this could be the case for arg-7-1 and arg-7-6.", "contents": "Arg-7 mutant X wild-type crosses in Chlamydomonas reinhardi: study of the enzyme produced in diploid strains. The arg-7 locus is the structural gene for the argininosuccinate lyase (ASL). Interallelic complementation was previously found to occur between several mutants of the locus: this is indicative for the homomultimeric nature of ASL. Two complementing (arg-7-5 and arg-7-7) and two non-complementing (arg-7-1 and arg-7-6) mutants of the arg-7 locus were crossed to the pab-2 strain (which is wild-type for the arg-7 locus). In each cross, heterozygote phenotypically wild-type strains were isolated; their diploid pattern was demonstrated by various criteria: mating type, cell volume, nuclear size. The four heterozygotes were compared to the haploid wild-type and in some experiments, to the diploid strain arg-1 X pab-2 homozygous for the arg-7 locus. No difference was found in growth rate and in the Michaelis constant values for ASL. The specific activity of the enzyme produced in the heterozygotes was about 50 percent of the activity found in haploid or diploid wild-type. The heat sensitivity of ASL was also investigated in the different strains: two (containing the complementing mutations arg-7-5 and arg-7-7) of the four heterozygotes produce ASL varieties different from the wild-type enzyme as far as the thermolability is concerned. These results suggest that hybrid ASL can be formed by interaction between the products of wild-type and mutant genes. A clear dominance of the wild-type allele is expected only when the mutant allele has no product of the gene: this could be the case for arg-7-1 and arg-7-6.", "PMID": 958202}
{"id": "pubmed23n0032_3004", "title": "Characterization of mutations in the penicillinase operon Staphylococcus aureus.", "content": "Mutant penicillinase plasmids, in which penicillinase synthesis is not inducible by penicillin or a penicillin analogue, were examined by biochemical and genetic analyses. In five of the six mutants tested, penicillinase synthesis could be induced by growth in the presence of 5-methyltryptophan. It is known that the tryptophan analogue 5-methyltryptophan is readily incorporated into protein by S. aureus and that staphylococcal penicillinase lacks tryptophan. 5-methyltryptophan seems to induce penicillinase synthesis in wild-type plasmids by becoming incorporated into the repressor and thereby inactivating the operator binding function of the penicillinase repressor. Therefore, induction of penicillinase synthesis in the mutant plasmids by 5-methyltryptophan strongly suggests that the noninducible phenotype of these five plasmids is due to a mutation that inactivates the effector binding site of the penicillinase repressor (i.e., the five mutant plasmids carry an iS genotype for the penicillinase repressor). This conclusion was supported by heterodiploid analysis. The mutant plasmid that did not respond to 5-methyltryptophan either produces an exceedingly low basal level of penicillinase or does not produce active enzyme. This plasmid seems to carry a mutation in the penicillinase structural gene or in the promoter for the structural gene. Thus, a genetic characterization of many mutations in the penicillinase operon can be accomplished easily and rapidly by biochemical analysis.", "contents": "Characterization of mutations in the penicillinase operon Staphylococcus aureus. Mutant penicillinase plasmids, in which penicillinase synthesis is not inducible by penicillin or a penicillin analogue, were examined by biochemical and genetic analyses. In five of the six mutants tested, penicillinase synthesis could be induced by growth in the presence of 5-methyltryptophan. It is known that the tryptophan analogue 5-methyltryptophan is readily incorporated into protein by S. aureus and that staphylococcal penicillinase lacks tryptophan. 5-methyltryptophan seems to induce penicillinase synthesis in wild-type plasmids by becoming incorporated into the repressor and thereby inactivating the operator binding function of the penicillinase repressor. Therefore, induction of penicillinase synthesis in the mutant plasmids by 5-methyltryptophan strongly suggests that the noninducible phenotype of these five plasmids is due to a mutation that inactivates the effector binding site of the penicillinase repressor (i.e., the five mutant plasmids carry an iS genotype for the penicillinase repressor). This conclusion was supported by heterodiploid analysis. The mutant plasmid that did not respond to 5-methyltryptophan either produces an exceedingly low basal level of penicillinase or does not produce active enzyme. This plasmid seems to carry a mutation in the penicillinase structural gene or in the promoter for the structural gene. Thus, a genetic characterization of many mutations in the penicillinase operon can be accomplished easily and rapidly by biochemical analysis.", "PMID": 958203}
{"id": "pubmed23n0032_3005", "title": "Non Xenopus-like DNA sequence organization in the Chironomus tentans genome.", "content": "We have examined the sequence organization of Chironomus tentans DNA by means of optical and hydroxyapatite renaturation kinetics of total DNA fragment sizes of 0.36, 2.6 and 13.5 kilobases (kb) as well as isolated middle repeat DNA at sizes of 0.36 and 13.5 kb. 90% of the DNA renatured as unique sequences of a genome of 0.20 pg with the balance of DNA renaturing as middle repetitive sequences present on averge 90 times per haploid genome. At a DNA fragment length of 13.5 kb, 35% of the DNA was trapped on the hydroxyapatite as middle repetiitive fraction. We concluded C. tentans DNA to have a mean repeat length of about 4.3 kb distributed throuth out at least 35% of the genome with an inter repeat spacing of at least 13.5 kb but possibly being distributed throughout the whole genome with an inter repeat spacing of 36 kb. This shows C. tentans DNA organization not to follow the most ubiquitous Xenopus model but to be similar to the organization of Drosophila melanogaster DNA.", "contents": "Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. We have examined the sequence organization of Chironomus tentans DNA by means of optical and hydroxyapatite renaturation kinetics of total DNA fragment sizes of 0.36, 2.6 and 13.5 kilobases (kb) as well as isolated middle repeat DNA at sizes of 0.36 and 13.5 kb. 90% of the DNA renatured as unique sequences of a genome of 0.20 pg with the balance of DNA renaturing as middle repetitive sequences present on averge 90 times per haploid genome. At a DNA fragment length of 13.5 kb, 35% of the DNA was trapped on the hydroxyapatite as middle repetiitive fraction. We concluded C. tentans DNA to have a mean repeat length of about 4.3 kb distributed throuth out at least 35% of the genome with an inter repeat spacing of at least 13.5 kb but possibly being distributed throughout the whole genome with an inter repeat spacing of 36 kb. This shows C. tentans DNA organization not to follow the most ubiquitous Xenopus model but to be similar to the organization of Drosophila melanogaster DNA.", "PMID": 958204}
{"id": "pubmed23n0032_3006", "title": "Kinetics of hydrogen-deuterium exchange of tryptophan and tryptophan peptides in deutero-trifluoroacetic acid using proton magenetic resonance spectroscopy.", "content": "The methods which have been used for the observation and assignment of resonances in the NMR spectra of proteins are reviewed. One such method, the selective deuteration of the aromatic protons of tryptophyl residues, is studied by NMR spectroscopy in model compounds in this paper, and in proteins in the following paper. On the basis of a reassignment of the PMR spectrum of the aromatic protons of L-tryptophan, the relative rates of H-D exchange in deutero-trifluoracetic acid (d-TFA) are H-2 greater than H-5 greater than H-6 greater than H-4 approximately H-7. The energies of activation for the first order exchange of both the H-2 and H-5 protons is 12 k.cal.mol-1. The rate constant for exchange of the H-2 protons of tryptophyl residues in peptides is much greater than in the amino acid itself and 5-10 times that for exchange of the H-5 protons. This suggests that the method can be used to label tryptophyl residues in proteins rapidly and specifically.", "contents": "Kinetics of hydrogen-deuterium exchange of tryptophan and tryptophan peptides in deutero-trifluoroacetic acid using proton magenetic resonance spectroscopy. The methods which have been used for the observation and assignment of resonances in the NMR spectra of proteins are reviewed. One such method, the selective deuteration of the aromatic protons of tryptophyl residues, is studied by NMR spectroscopy in model compounds in this paper, and in proteins in the following paper. On the basis of a reassignment of the PMR spectrum of the aromatic protons of L-tryptophan, the relative rates of H-D exchange in deutero-trifluoracetic acid (d-TFA) are H-2 greater than H-5 greater than H-6 greater than H-4 approximately H-7. The energies of activation for the first order exchange of both the H-2 and H-5 protons is 12 k.cal.mol-1. The rate constant for exchange of the H-2 protons of tryptophyl residues in peptides is much greater than in the amino acid itself and 5-10 times that for exchange of the H-5 protons. This suggests that the method can be used to label tryptophyl residues in proteins rapidly and specifically.", "PMID": 958213}
{"id": "pubmed23n0032_3007", "title": "The glycerophosphateacyltransferases and their function in the metabolism of fatty acids.", "content": "1. From different studies on the cellular localization, postional specificity, and regulatory properties of acyl-CoA: glycerophosphate acyltransferase (EC 2,3,1.15) AND ACYL-CoA: 1-ACYLGLYCEROPHOSPHATE ACYLTRANSFERASE (EC 2,3,1....) the following conclusions can be drawn: The glycerophosphate acyltransferase is localized in the endoplasmatic reticulum (microsomes) and in the outer membrane of the mitochondria of the animal cell. Its reaction product is 1-acylglycerophosphate (1-lysophosphatidic acid). The mitochondrial enzyme shows a high preference for saturated fatty acids while the microsomal enzyme is less specific (alternatively the microsomes contain more than one glycerophsophate acyltransferase). 2. The 1-acylglycerphosphate acyltransferase is localized in the endoplasmatic reticulum (microsomes) in the animal cell. Possibly a minor fraction of this enzyme is localized to the outer membrane of the mitochondria. This enzyme shows a strong preference for unsaturated fatty acids. 3. Both the microsomal and the mitochondrial dihydroxyacetonephosphate acyltransferase show similar fatty acid specificity as the corresponding glycerophosphate acyltransferases. It cannot be excluded that dihydroxy-acetonephosphate and glycerophosphate are acylated by the same enzymes. 4. The activity of the glycerophosphate acyltransferase(s) in the liver decreases in fasting or fat feeding and increases upon feeding of carbohydrate. The activity of carnitine palmityltransferase varies exacty opposit. These enzymes do not show dietary variations in heart and adipose tissue. 5. Under the otherwise identical conditions the rate of carnitine acylation in isolated mitochondria decreases more than the rate of glycerophosphate acylation when the concentration of palmityl-CoA is reduced. 6. In isolated liver cells (which has lost most of their carnitine) addition of carnitine increases the rate of fatty acid oxidation and decreases the rate of triglyceride formation. 7. Glycerol and fructose lower the rate of fatty acid oxidation, probably by lowering the levels of acyl-CoA and acyl-carnitine in the cells. 8. It is concluded that the relative activities of glycerophosphate acyltranse and carnitine palmityltransferase probably influence the fate of fatty acids in the cell.", "contents": "The glycerophosphateacyltransferases and their function in the metabolism of fatty acids. 1. From different studies on the cellular localization, postional specificity, and regulatory properties of acyl-CoA: glycerophosphate acyltransferase (EC 2,3,1.15) AND ACYL-CoA: 1-ACYLGLYCEROPHOSPHATE ACYLTRANSFERASE (EC 2,3,1....) the following conclusions can be drawn: The glycerophosphate acyltransferase is localized in the endoplasmatic reticulum (microsomes) and in the outer membrane of the mitochondria of the animal cell. Its reaction product is 1-acylglycerophosphate (1-lysophosphatidic acid). The mitochondrial enzyme shows a high preference for saturated fatty acids while the microsomal enzyme is less specific (alternatively the microsomes contain more than one glycerophsophate acyltransferase). 2. The 1-acylglycerphosphate acyltransferase is localized in the endoplasmatic reticulum (microsomes) in the animal cell. Possibly a minor fraction of this enzyme is localized to the outer membrane of the mitochondria. This enzyme shows a strong preference for unsaturated fatty acids. 3. Both the microsomal and the mitochondrial dihydroxyacetonephosphate acyltransferase show similar fatty acid specificity as the corresponding glycerophosphate acyltransferases. It cannot be excluded that dihydroxy-acetonephosphate and glycerophosphate are acylated by the same enzymes. 4. The activity of the glycerophosphate acyltransferase(s) in the liver decreases in fasting or fat feeding and increases upon feeding of carbohydrate. The activity of carnitine palmityltransferase varies exacty opposit. These enzymes do not show dietary variations in heart and adipose tissue. 5. Under the otherwise identical conditions the rate of carnitine acylation in isolated mitochondria decreases more than the rate of glycerophosphate acylation when the concentration of palmityl-CoA is reduced. 6. In isolated liver cells (which has lost most of their carnitine) addition of carnitine increases the rate of fatty acid oxidation and decreases the rate of triglyceride formation. 7. Glycerol and fructose lower the rate of fatty acid oxidation, probably by lowering the levels of acyl-CoA and acyl-carnitine in the cells. 8. It is concluded that the relative activities of glycerophosphate acyltranse and carnitine palmityltransferase probably influence the fate of fatty acids in the cell.", "PMID": 958214}
{"id": "pubmed23n0032_3008", "title": "The synthesis of alpha-amylase by rough and in vitro reconstituted rough endoplasmic reticulum derived from rat parotid gland.", "content": "The isolation of rough and smooth endoplasmic reticulum from rat parotid salivary gland is described. The rough membrane was stripped of its bound ribosomes using the KCl-puromycin method. Rough endoplasmic reticulum was reconstituted from stripped-rough membrane and polyribosomes. The reconstituted rough membrane resembled the native rough membrane in the following aspects: RNA/protein ratio, buoyant density in a continuous sucrose gradient and amino acid incorporation capacity. The in vitro synthesis of alpha-amylase by both rough and in vitro reconstituted rough membrane was demonstrated using SDS polyacrylamide gel electrophoresis. The reconstituted rough membrane could be restripped by KCl-puromycin. The in vitro synthesized alpha-amylase remained associated with the rough or the in vitro reconstituted rough membrane, even after these membranes were stripped of their bound ribosomes.", "contents": "The synthesis of alpha-amylase by rough and in vitro reconstituted rough endoplasmic reticulum derived from rat parotid gland. The isolation of rough and smooth endoplasmic reticulum from rat parotid salivary gland is described. The rough membrane was stripped of its bound ribosomes using the KCl-puromycin method. Rough endoplasmic reticulum was reconstituted from stripped-rough membrane and polyribosomes. The reconstituted rough membrane resembled the native rough membrane in the following aspects: RNA/protein ratio, buoyant density in a continuous sucrose gradient and amino acid incorporation capacity. The in vitro synthesis of alpha-amylase by both rough and in vitro reconstituted rough membrane was demonstrated using SDS polyacrylamide gel electrophoresis. The reconstituted rough membrane could be restripped by KCl-puromycin. The in vitro synthesized alpha-amylase remained associated with the rough or the in vitro reconstituted rough membrane, even after these membranes were stripped of their bound ribosomes.", "PMID": 958215}
{"id": "pubmed23n0032_3009", "title": "Binding of aminoacyl-tRNA to rat liver ribosomal proteins.", "content": "Rat liver ribosome treatment with ethanol and 1 M NH4Cl releases some 31-33 ribosomal proteins. This split protein fraction binds Phe-tRNA, Ac-Phe-tRNA, Met-tRNAM and f-Met-tRNAF in the absence of K+ and Mg++ ions. When the split protein fraction is passed through Sephadex G-100 only six proteins are retained in the column: S10, S14, S15, S19, L35, and L36. The aminoacyl-tRNA binding activity of this protein fraction retained in the Sephadex G-100 column is similar to that of the total split protein fraction, suggesting that the above six proteins, or only some of them, are involved in the binding reaction.", "contents": "Binding of aminoacyl-tRNA to rat liver ribosomal proteins. Rat liver ribosome treatment with ethanol and 1 M NH4Cl releases some 31-33 ribosomal proteins. This split protein fraction binds Phe-tRNA, Ac-Phe-tRNA, Met-tRNAM and f-Met-tRNAF in the absence of K+ and Mg++ ions. When the split protein fraction is passed through Sephadex G-100 only six proteins are retained in the column: S10, S14, S15, S19, L35, and L36. The aminoacyl-tRNA binding activity of this protein fraction retained in the Sephadex G-100 column is similar to that of the total split protein fraction, suggesting that the above six proteins, or only some of them, are involved in the binding reaction.", "PMID": 958216}
{"id": "pubmed23n0032_3010", "title": "Distribution of globin genes in chicken reticulocyte chromatin fractionated on urografin gradients.", "content": "Buoyant density centrifugation in Urografin solutions resolved French Pressure Cell-sheared, and micrococcal nuclease-digested avian reticulocyte chromatin into a broad profile of two peaks. Hybridization experiments using a globin cDNA probe suggested minimal fractionation of transcriptionally active and inactive components with chromatin sheared at 6000 psi, while no evidence was obtained for any fractionation with chromatin sheared at lower or higher pressures, or with chromatin digested to various extents with micrococcal nuclease, despite a considerable spread of chromatin material across gradients.", "contents": "Distribution of globin genes in chicken reticulocyte chromatin fractionated on urografin gradients. Buoyant density centrifugation in Urografin solutions resolved French Pressure Cell-sheared, and micrococcal nuclease-digested avian reticulocyte chromatin into a broad profile of two peaks. Hybridization experiments using a globin cDNA probe suggested minimal fractionation of transcriptionally active and inactive components with chromatin sheared at 6000 psi, while no evidence was obtained for any fractionation with chromatin sheared at lower or higher pressures, or with chromatin digested to various extents with micrococcal nuclease, despite a considerable spread of chromatin material across gradients.", "PMID": 958217}
{"id": "pubmed23n0032_3011", "title": "Further studies on collagen mRNA: partial chemical characterization and polyadenylic acid sequence.", "content": "Collagen mRNA has already been purified and characterized by us. Its purity has now been enhanced by two different methods. Gel electrophoresis shows in either method, a single peak with the same mobility already reported: 1.05 X 10(6) daltons. Base composition analyses of collagen mRNA purified by either method were almost identical. Chemical analyses of the isolated polyadenylic acid stretch show that it is, 0.48 X 10(5) daltons-long, (about 140 nucleotides-long), contains 75% AMP, and is located at the 3' end of the polymer.", "contents": "Further studies on collagen mRNA: partial chemical characterization and polyadenylic acid sequence. Collagen mRNA has already been purified and characterized by us. Its purity has now been enhanced by two different methods. Gel electrophoresis shows in either method, a single peak with the same mobility already reported: 1.05 X 10(6) daltons. Base composition analyses of collagen mRNA purified by either method were almost identical. Chemical analyses of the isolated polyadenylic acid stretch show that it is, 0.48 X 10(5) daltons-long, (about 140 nucleotides-long), contains 75% AMP, and is located at the 3' end of the polymer.", "PMID": 958218}
{"id": "pubmed23n0032_3012", "title": "[mRNA fractions of mouse plasmacytoma cells].", "content": "The sedimentation characteristics of polysomal mRNA labelled in vitro by [5-3H]uridine and electrophoretic mobility of similar non-labelled mRNA of mouse plasmacytoma were studied. Rapidly labelled polysomal mRNA may be considered as mRNA on the basis of several independent but indirect tests. These mRNA's are localized in 18-6S region of sucrose gradient. Some part of radioactivity have been found in the ribosomal RNA. It was shown that there is 8-10 RNA fractions in sucrose gradient. The 16S and 12-14S fractions are isolated and partially purified by two- and three-fold centrifugation. Fractions homogenous in sucrose gradient were electrophoresed in PAAG-SDS and divided into several subfractions some of them being common for 16S and 12-14S. The number of non-crossover subfractions was about 2-3. Not less than 20 different main fractions of polysomal mRNA were determined in plasmacytoma cells on the basis of electrophoretic data.", "contents": "[mRNA fractions of mouse plasmacytoma cells]. The sedimentation characteristics of polysomal mRNA labelled in vitro by [5-3H]uridine and electrophoretic mobility of similar non-labelled mRNA of mouse plasmacytoma were studied. Rapidly labelled polysomal mRNA may be considered as mRNA on the basis of several independent but indirect tests. These mRNA's are localized in 18-6S region of sucrose gradient. Some part of radioactivity have been found in the ribosomal RNA. It was shown that there is 8-10 RNA fractions in sucrose gradient. The 16S and 12-14S fractions are isolated and partially purified by two- and three-fold centrifugation. Fractions homogenous in sucrose gradient were electrophoresed in PAAG-SDS and divided into several subfractions some of them being common for 16S and 12-14S. The number of non-crossover subfractions was about 2-3. Not less than 20 different main fractions of polysomal mRNA were determined in plasmacytoma cells on the basis of electrophoretic data.", "PMID": 958220}
{"id": "pubmed23n0032_3013", "title": "[Kinetic patterns of reactions with immobilized enzymes in open systems].", "content": "For studying the properties of immobilized enzyme, a flow reactor is suggested which provides ideal mixing of the reaction components with the granules of immobilized enzyme. To elucidate the mechanism of the reaction and to determine the effective values of maximal rates and Michaelis constants, an analysis of formal kinetic relationships describing the behaviour of the reactor in the stationary and non-stationary regimes has been carried out. The non-stationary kinetics is analyzed both for the case of step-wise and continuous supply of the substrate. A number of equations has been evolved which describe the time dependences of the concentrations of the substrate and reaction products; relationships have been found allowing determination of maximal rates and Michaelis constants from experimental data.", "contents": "[Kinetic patterns of reactions with immobilized enzymes in open systems]. For studying the properties of immobilized enzyme, a flow reactor is suggested which provides ideal mixing of the reaction components with the granules of immobilized enzyme. To elucidate the mechanism of the reaction and to determine the effective values of maximal rates and Michaelis constants, an analysis of formal kinetic relationships describing the behaviour of the reactor in the stationary and non-stationary regimes has been carried out. The non-stationary kinetics is analyzed both for the case of step-wise and continuous supply of the substrate. A number of equations has been evolved which describe the time dependences of the concentrations of the substrate and reaction products; relationships have been found allowing determination of maximal rates and Michaelis constants from experimental data.", "PMID": 958221}
{"id": "pubmed23n0032_3014", "title": "Naturally occuring mutagens.", "content": "Naturally occurring mutagens have usually been discovered as a result of outbreaks of disease in agricultural livestock, or as a result of epidemiological studies of cancer of the liver in man. Subsequent work has then shown that the toxic agents responsible often have mutagenic properties. Examples are the pyrrolizidine alkaloids, cyasin, a range of mycotoxins produced by various fungi, and at least two unidentified toxic agents in bracken. Commonly the toxic agent itself does not show high biological activity, but after ingestion it is converted by metabolic processes into the active mutagen or carcinogen. Some of these toxic substances have been responsible for considerable losses of agricultural livestock and therefore are of economic significance. From the view-point of genetic hazards to man, the most significant compounds are probably the mycotoxins, e.g. aflatoxin, because of the common risk of fungal contamination of food, especially in tropical regions. No information is yet available on the effects of these mutagens on natural populations of animals. Plants containing the pyrrolizidine alkaloids are found in areas of Africa and the Middle East where plagues of the migratory locust occur. Although it is known that some of the alkaloids can induce chromosomal damage in grasshoppers, whether such damage ever becomes a significant factor under ecological conditions is not known. In some cases, insects have not only evolved resistance towards mutagenic alkaloids but have become dependent on them for certain purposes. The males of certain Danaid butterflies feed on plants containing pyrrolizidine alkaloids. After ingestion, the alkaloids are metabolised to dihydropyrrolizine derivatives, which are then secreted on special organs (hair pencils) and, following dissemination into the atmosphere, act as pheromones for the stimulation of mating behaviour.", "contents": "Naturally occuring mutagens. Naturally occurring mutagens have usually been discovered as a result of outbreaks of disease in agricultural livestock, or as a result of epidemiological studies of cancer of the liver in man. Subsequent work has then shown that the toxic agents responsible often have mutagenic properties. Examples are the pyrrolizidine alkaloids, cyasin, a range of mycotoxins produced by various fungi, and at least two unidentified toxic agents in bracken. Commonly the toxic agent itself does not show high biological activity, but after ingestion it is converted by metabolic processes into the active mutagen or carcinogen. Some of these toxic substances have been responsible for considerable losses of agricultural livestock and therefore are of economic significance. From the view-point of genetic hazards to man, the most significant compounds are probably the mycotoxins, e.g. aflatoxin, because of the common risk of fungal contamination of food, especially in tropical regions. No information is yet available on the effects of these mutagens on natural populations of animals. Plants containing the pyrrolizidine alkaloids are found in areas of Africa and the Middle East where plagues of the migratory locust occur. Although it is known that some of the alkaloids can induce chromosomal damage in grasshoppers, whether such damage ever becomes a significant factor under ecological conditions is not known. In some cases, insects have not only evolved resistance towards mutagenic alkaloids but have become dependent on them for certain purposes. The males of certain Danaid butterflies feed on plants containing pyrrolizidine alkaloids. After ingestion, the alkaloids are metabolised to dihydropyrrolizine derivatives, which are then secreted on special organs (hair pencils) and, following dissemination into the atmosphere, act as pheromones for the stimulation of mating behaviour.", "PMID": 958226}
{"id": "pubmed23n0032_3015", "title": "Localized mutagenesis in Streptomyces coelicolor A3 (2).", "content": "Nutritional mutants (co-mutants) were scored among nitrosoguanidine-induced revertants of four mutations in Streptomyces coelicolor A3 (2). All co-mutations were due to mutations in genes linked to the revertant locus. The co-mutant loci were located in a region of about 20 map units around the revertant locus (co-mutation region). Revertants for different loci showed co-mutation patterns different from each other and from that of random nitrosoguanidine-induced forward mutations. Mutations appeared to be completely abolished outside the co-mutation region (mutation restriction).", "contents": "Localized mutagenesis in Streptomyces coelicolor A3 (2). Nutritional mutants (co-mutants) were scored among nitrosoguanidine-induced revertants of four mutations in Streptomyces coelicolor A3 (2). All co-mutations were due to mutations in genes linked to the revertant locus. The co-mutant loci were located in a region of about 20 map units around the revertant locus (co-mutation region). Revertants for different loci showed co-mutation patterns different from each other and from that of random nitrosoguanidine-induced forward mutations. Mutations appeared to be completely abolished outside the co-mutation region (mutation restriction).", "PMID": 958227}
{"id": "pubmed23n0032_3016", "title": "[Study of ribonucleoprotein particles by the method of RNA chromatography on a column of nucleoprotein-celite].", "content": "A novel method of RNA fractionation based on a gradual release of the RNA molecules from ribonucleoprotein complexes has been used for the analysis of ribosomal and non-ribosomal complexes of rat liver cytoplasm. Adsorption of native ribonucleoproteins on a Celite column (occuring through only the protein moiety) followed by a consequent dissociation of RNP complexes brought about by various agents results in RNA fractionation in accordance with the tightness of the RNA-protein bonds. The cytoplasmic ribosomal and rapidly labelled non-ribosomal RNA species are separated into several fractions identified as 18S and 28S rRNA's, mRNA and messenger-like RNA. A relatively small fraction (about 10% of the total) of rRNA tenaciously bound to protein has been also revealed.", "contents": "[Study of ribonucleoprotein particles by the method of RNA chromatography on a column of nucleoprotein-celite]. A novel method of RNA fractionation based on a gradual release of the RNA molecules from ribonucleoprotein complexes has been used for the analysis of ribosomal and non-ribosomal complexes of rat liver cytoplasm. Adsorption of native ribonucleoproteins on a Celite column (occuring through only the protein moiety) followed by a consequent dissociation of RNP complexes brought about by various agents results in RNA fractionation in accordance with the tightness of the RNA-protein bonds. The cytoplasmic ribosomal and rapidly labelled non-ribosomal RNA species are separated into several fractions identified as 18S and 28S rRNA's, mRNA and messenger-like RNA. A relatively small fraction (about 10% of the total) of rRNA tenaciously bound to protein has been also revealed.", "PMID": 958223}
{"id": "pubmed23n0032_3017", "title": "The radiosensitivities of lymphocytes from pig, sheep, goat and cow.", "content": "Dose-effect curves for X-irradiation in vitro were determined on cultured blood lymphocytes of four mammalian species-cow, goat, sheep and pig-having approximately the same number of chromosome arms, the same nuclear volume and the same DNA content. The data from cow, but not those from the other species, exhibited significant inhomogeneity between the experiments. On inspection the numerical yields appear not to differ greatly in the lower dose range. Evaluation of the parameters according to the quadratic law and the power law, however, demonstrate significant differences. Pig differs from the other species regardless of which presentation is chosen. For the power law a significant difference is also found between goat and sheep.", "contents": "The radiosensitivities of lymphocytes from pig, sheep, goat and cow. Dose-effect curves for X-irradiation in vitro were determined on cultured blood lymphocytes of four mammalian species-cow, goat, sheep and pig-having approximately the same number of chromosome arms, the same nuclear volume and the same DNA content. The data from cow, but not those from the other species, exhibited significant inhomogeneity between the experiments. On inspection the numerical yields appear not to differ greatly in the lower dose range. Evaluation of the parameters according to the quadratic law and the power law, however, demonstrate significant differences. Pig differs from the other species regardless of which presentation is chosen. For the power law a significant difference is also found between goat and sheep.", "PMID": 958228}
{"id": "pubmed23n0032_3018", "title": "X-Ray-induced translocations in spermatoginia. II. Fractionation in mice.", "content": "The dose-response curve for reciprocal translocations induced by X-rays in spermatogonial stem cells, and observed in primary spermatocytes of mice, is \"hump-shaped\", with a maximum yield at about 600 R. To test the hypothesis that the decrease in yield with increasing dose above 600 R is a consequence of the different sensitivities of cells in different stages of the cell cycle to both cell killing and chromosome aberration induction, several fractionation experiments were carried out. A total  dose of 2800 R was given in repeated doses of 400 R, separated by 8-week intervals. The yield of translocations is that expected for additivity; for example, the yield at 1600 R is approximately equal to that for four separate 400-R doses. When a total dose (500 R) which gives a translocation yield on the ascending part of the dose-response curve is given as two equal fractions separated by intervals of 30, 90, or 150 min, the translocation yield decreases with increasing interval. However, when a total dose (1000 R) which would give a translocation yield on the descending part of the dose-response curve is given in two equal fractions separated by intervals of from 30 min to 6 weeks, the response is different; the translocation yield increases with intervals up to 18 h, then decreases with intervals up to 4 weeks, and finally increases again to a yield equal to additivity with an interval of 6 weeks. These changes in translocation yield with changes in interval between the two doses are explained in terms of the differential sensitivity of cells to killing and aberration induction in the different phases of the cell cycle, and by assuming that the cells surviving the first dose and repopulating the testis have different cycle characteristics from normal cells.", "contents": "X-Ray-induced translocations in spermatoginia. II. Fractionation in mice. The dose-response curve for reciprocal translocations induced by X-rays in spermatogonial stem cells, and observed in primary spermatocytes of mice, is \"hump-shaped\", with a maximum yield at about 600 R. To test the hypothesis that the decrease in yield with increasing dose above 600 R is a consequence of the different sensitivities of cells in different stages of the cell cycle to both cell killing and chromosome aberration induction, several fractionation experiments were carried out. A total  dose of 2800 R was given in repeated doses of 400 R, separated by 8-week intervals. The yield of translocations is that expected for additivity; for example, the yield at 1600 R is approximately equal to that for four separate 400-R doses. When a total dose (500 R) which gives a translocation yield on the ascending part of the dose-response curve is given as two equal fractions separated by intervals of 30, 90, or 150 min, the translocation yield decreases with increasing interval. However, when a total dose (1000 R) which would give a translocation yield on the descending part of the dose-response curve is given in two equal fractions separated by intervals of from 30 min to 6 weeks, the response is different; the translocation yield increases with intervals up to 18 h, then decreases with intervals up to 4 weeks, and finally increases again to a yield equal to additivity with an interval of 6 weeks. These changes in translocation yield with changes in interval between the two doses are explained in terms of the differential sensitivity of cells to killing and aberration induction in the different phases of the cell cycle, and by assuming that the cells surviving the first dose and repopulating the testis have different cycle characteristics from normal cells.", "PMID": 958229}
{"id": "pubmed23n0032_3019", "title": "[Inhibition of Aspergillus clavatus intracellular RNA-ase by nucleotides and the components comprising them].", "content": "The constants of inhibition by the nucleotides and constituting components catalysed by intracellular \"acid\" (pHopt 4.7), non-specific RNAse from Asp. clavatus (EC 3.1.4.23) were determined.. All the nucleotides were found to be competitive inhibitors. The influence of composition and structure of the nucleotides on their interaction with the active site of RNAse is discussed.", "contents": "[Inhibition of Aspergillus clavatus intracellular RNA-ase by nucleotides and the components comprising them]. The constants of inhibition by the nucleotides and constituting components catalysed by intracellular \"acid\" (pHopt 4.7), non-specific RNAse from Asp. clavatus (EC 3.1.4.23) were determined.. All the nucleotides were found to be competitive inhibitors. The influence of composition and structure of the nucleotides on their interaction with the active site of RNAse is discussed.", "PMID": 958225}
{"id": "pubmed23n0032_3020", "title": "The influence of serum components on the growth and mutation of Chinese hamster cells in medium containing 8-azaguanine.", "content": "Low concentrations (less than or equal 20 mug/ml) of 8-azaguanine are 1000 fold more toxic to V79 Chinese hamster cells in medium containing 10% dialyzed fetal calf serum than in medium containing 10% undialyzed serum. Serum enzyme activity that converts AG to nontoxic 8-azaxanthine degrades AG at the same rate, whether or not the serum is dialyzed. However, cytotoxicity results similar to those obtained with US were produced in medium containing DS and 2.5 mug of hypoxanthine (HX)/ml (DSH). Therefore, serum HX is considered to be responsible for the relatively low cytotoxicity of AG in medium containing US. Colonies that arose in medium containing AG were isolated and characterized. Those that remained resistant to AG (40 mug/ml) and sensitive to aminopterin in the presence of HX and thymidine (HAT) were considered mutants; non-mutants were sensitive to AG and resistant to HAT. Colonies isolated from medium containing DSH or US and low concentrations of AG were not mutants, but those from medium containing high concentrations (greater than or equal 30 mug/ml) of AG were mutants. Spontaneous and N-methyl-N-nitro-N-nitrosoguanidine induced mutants were detectable in medium containing DSH without replating the cells prior to adding AG (greater than or equal 30 mug/ml), but in order to detect MNNG induced mutations in medium containing DS replating was essential. In DS, the mutation frequency increased as an exponential function of the toxicity of MNNG, but remained two orders of magnitude lower than the induced mutation frequencies that occurred in DSH. HX, in DSH or US, produced profound effects, other than interference with AG toxicity, that distort the results of mutagenesis assays. To study mutation using AG resistance as the endpoint, it is essential to use dialyzed serum.", "contents": "The influence of serum components on the growth and mutation of Chinese hamster cells in medium containing 8-azaguanine. Low concentrations (less than or equal 20 mug/ml) of 8-azaguanine are 1000 fold more toxic to V79 Chinese hamster cells in medium containing 10% dialyzed fetal calf serum than in medium containing 10% undialyzed serum. Serum enzyme activity that converts AG to nontoxic 8-azaxanthine degrades AG at the same rate, whether or not the serum is dialyzed. However, cytotoxicity results similar to those obtained with US were produced in medium containing DS and 2.5 mug of hypoxanthine (HX)/ml (DSH). Therefore, serum HX is considered to be responsible for the relatively low cytotoxicity of AG in medium containing US. Colonies that arose in medium containing AG were isolated and characterized. Those that remained resistant to AG (40 mug/ml) and sensitive to aminopterin in the presence of HX and thymidine (HAT) were considered mutants; non-mutants were sensitive to AG and resistant to HAT. Colonies isolated from medium containing DSH or US and low concentrations of AG were not mutants, but those from medium containing high concentrations (greater than or equal 30 mug/ml) of AG were mutants. Spontaneous and N-methyl-N-nitro-N-nitrosoguanidine induced mutants were detectable in medium containing DSH without replating the cells prior to adding AG (greater than or equal 30 mug/ml), but in order to detect MNNG induced mutations in medium containing DS replating was essential. In DS, the mutation frequency increased as an exponential function of the toxicity of MNNG, but remained two orders of magnitude lower than the induced mutation frequencies that occurred in DSH. HX, in DSH or US, produced profound effects, other than interference with AG toxicity, that distort the results of mutagenesis assays. To study mutation using AG resistance as the endpoint, it is essential to use dialyzed serum.", "PMID": 958230}
{"id": "pubmed23n0032_3021", "title": "[Molecular organization of aggregated forms of chlorophyll and its analogs].", "content": "Infrared spectra (in the region of 1800-1600 cm-1) of aggregated protochlorophyll, chlorophyll a and b, bacterioviridin, protopheophytin, pheophytin a (in the solid films) with different absorption spectra were studied. The formation of various types of aggregates is due to the realization of different modes of intermolecular bonding. The mode of interaction depends upon the chemical structure of the pigment and upon the inclusion of low-molecular addenda into the structure of aggregates. The forms 677 nm of chlorophyll a and 658 nm of chlorophyll b appear when the coordination bonds between the keto groups of the cyclopentanone ring and central magnesium atom are formed. The longwave forms of chlorophyll a (745 nm) and chlorophyll b (675 nm, 725 nm) are formed when methanol molecules are involved in the intermolecular bonds. The coordination bonds between C=O and magnesium atom in the longwave aggregate of bacterioviridin (750 nm) are observed without participation of the low-molecular addenda, The formation of the protochlorophyl 635 nm form and aggregated forms of magnesium-free pigments occurs without participation of carbonyl groups probably by direct interaction between the eta-electron systems of molecules. The frequencies of carbonyl groups of the solid films in dioxane vapours causing absorption maxima at 690 nm (chlorophyll a) and 670 nm (chlorophyll b) are not shifted as compared to the dissolved pigments. Thipoints to the intermolecular interaction without participation of C=O groups.", "contents": "[Molecular organization of aggregated forms of chlorophyll and its analogs]. Infrared spectra (in the region of 1800-1600 cm-1) of aggregated protochlorophyll, chlorophyll a and b, bacterioviridin, protopheophytin, pheophytin a (in the solid films) with different absorption spectra were studied. The formation of various types of aggregates is due to the realization of different modes of intermolecular bonding. The mode of interaction depends upon the chemical structure of the pigment and upon the inclusion of low-molecular addenda into the structure of aggregates. The forms 677 nm of chlorophyll a and 658 nm of chlorophyll b appear when the coordination bonds between the keto groups of the cyclopentanone ring and central magnesium atom are formed. The longwave forms of chlorophyll a (745 nm) and chlorophyll b (675 nm, 725 nm) are formed when methanol molecules are involved in the intermolecular bonds. The coordination bonds between C=O and magnesium atom in the longwave aggregate of bacterioviridin (750 nm) are observed without participation of the low-molecular addenda, The formation of the protochlorophyl 635 nm form and aggregated forms of magnesium-free pigments occurs without participation of carbonyl groups probably by direct interaction between the eta-electron systems of molecules. The frequencies of carbonyl groups of the solid films in dioxane vapours causing absorption maxima at 690 nm (chlorophyll a) and 670 nm (chlorophyll b) are not shifted as compared to the dissolved pigments. Thipoints to the intermolecular interaction without participation of C=O groups.", "PMID": 958222}
{"id": "pubmed23n0032_3022", "title": "Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cells.", "content": "Resistance to 6-thioguanine was induced by 5-bromodeoxyuridine (BUdR) in synchronous Chinese hamster cells. The yield of mutant colonies was not proportional to the amount of BUdR incorporated into DNA; thus mutants were not due to mispairing of BUdR with guanine during replication. Few mutants were induced until BUdR concentrations exceeded that of the intracellular thymidine triphosphate pool and mutant yield was depressed by addition of thymidine to the medium. These data suggest that BUdR exerts an allosteric effect on the DNA synthesizing system which renders it more error prone.", "contents": "Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cells. Resistance to 6-thioguanine was induced by 5-bromodeoxyuridine (BUdR) in synchronous Chinese hamster cells. The yield of mutant colonies was not proportional to the amount of BUdR incorporated into DNA; thus mutants were not due to mispairing of BUdR with guanine during replication. Few mutants were induced until BUdR concentrations exceeded that of the intracellular thymidine triphosphate pool and mutant yield was depressed by addition of thymidine to the medium. These data suggest that BUdR exerts an allosteric effect on the DNA synthesizing system which renders it more error prone.", "PMID": 958231}
{"id": "pubmed23n0032_3023", "title": "Radiation-induced chromosomal aberrations in human lymphocytes. I. Dependence on the dose of gamma-rays and an anomaly at low doses.", "content": "Cultures of human lymphocytes obtained from blood of healthy adult donors were irradiated with different doses of 60Co gamma-rays and the irradiated cells were analysed in metaphase 50 h after irradiation. The effect (total yield of aberrations of chromosome type, or total yield of exchange type aberrations) produced by the lowest dose (5 rad) appears to be statistically significant in a sample of 1500 cells. In the usual dose range (25-400 rad), both parabolic and linear-quadratic equations give a satisfactory fit of experimental data (dicetrics, fragments, or all aberrations of chromosome type). Low doses of gamma-rays, however, produced more aberrations than expected, if one extrapolates dose-effect curves from higher doses. Both relations should be considered, therefore, merely as empirical equations. Dicentrics show at low doses (10-30 rad) a plateau which appears to be statistically significant. Some indications are obtained that the total number of chromosome-type aberrations is a more reliable criterion of cytogenetic damage than the usually accepted yield of dicentrics and rings.", "contents": "Radiation-induced chromosomal aberrations in human lymphocytes. I. Dependence on the dose of gamma-rays and an anomaly at low doses. Cultures of human lymphocytes obtained from blood of healthy adult donors were irradiated with different doses of 60Co gamma-rays and the irradiated cells were analysed in metaphase 50 h after irradiation. The effect (total yield of aberrations of chromosome type, or total yield of exchange type aberrations) produced by the lowest dose (5 rad) appears to be statistically significant in a sample of 1500 cells. In the usual dose range (25-400 rad), both parabolic and linear-quadratic equations give a satisfactory fit of experimental data (dicetrics, fragments, or all aberrations of chromosome type). Low doses of gamma-rays, however, produced more aberrations than expected, if one extrapolates dose-effect curves from higher doses. Both relations should be considered, therefore, merely as empirical equations. Dicentrics show at low doses (10-30 rad) a plateau which appears to be statistically significant. Some indications are obtained that the total number of chromosome-type aberrations is a more reliable criterion of cytogenetic damage than the usually accepted yield of dicentrics and rings.", "PMID": 958232}
{"id": "pubmed23n0032_3024", "title": "[Calculation of the conformation of stereo-regular, double-spiral polynucleotides].", "content": "By the step-by-step selection method energetically optimal conformation of double-stranded polynucleotides were calculated proceeding from the conformations of mono-and dinucleotides. These structures were found to belong to the families of DNA A and B forms. The dependence of six torsion angles of the polynucleotide chain on the glycosidic angle chi was investigated. As a result certain areas admitting of double-helix polynucleotides with sugar C3-endo with chi = -80 degrees were established.", "contents": "[Calculation of the conformation of stereo-regular, double-spiral polynucleotides]. By the step-by-step selection method energetically optimal conformation of double-stranded polynucleotides were calculated proceeding from the conformations of mono-and dinucleotides. These structures were found to belong to the families of DNA A and B forms. The dependence of six torsion angles of the polynucleotide chain on the glycosidic angle chi was investigated. As a result certain areas admitting of double-helix polynucleotides with sugar C3-endo with chi = -80 degrees were established.", "PMID": 958224}
{"id": "pubmed23n0032_3025", "title": "Mutagenicity and toxicity of amitrole. II. Human lymphocyte culture tests.", "content": "Effects of amitrole (3-amino-1,2,4-triazole) on human leucocytes in culture were investigated. Amitrole interfered with lymphoblast transformation and inhibited cell growth in concentrations of 0.2% w/v and higher. Selected metaphases were examined for the presence of chromosome and chromatid aberrations. No clastogenic effects were observed.", "contents": "Mutagenicity and toxicity of amitrole. II. Human lymphocyte culture tests. Effects of amitrole (3-amino-1,2,4-triazole) on human leucocytes in culture were investigated. Amitrole interfered with lymphoblast transformation and inhibited cell growth in concentrations of 0.2% w/v and higher. Selected metaphases were examined for the presence of chromosome and chromatid aberrations. No clastogenic effects were observed.", "PMID": 958234}
{"id": "pubmed23n0032_3026", "title": "Chromosome damage induced by semicarbazide in spermatocytes of a grasshopper.", "content": "Semicarbazide hydrochloride (0.1 M in glass-distilled water), on injection, showed mutagenic action on the spermatocyte chromosomes of the grasshopper, Spathosternum prasiniferum. Aberrations such as chromatid and chromosome breaks, translocations, fragments and bridges were encountered. The sex chromosome and the long autosomes were affected. Semicarbazide, perhaps, reacts with DNA and the chromosome in a way similar to that of hydroxylamine and hydrazines.", "contents": "Chromosome damage induced by semicarbazide in spermatocytes of a grasshopper. Semicarbazide hydrochloride (0.1 M in glass-distilled water), on injection, showed mutagenic action on the spermatocyte chromosomes of the grasshopper, Spathosternum prasiniferum. Aberrations such as chromatid and chromosome breaks, translocations, fragments and bridges were encountered. The sex chromosome and the long autosomes were affected. Semicarbazide, perhaps, reacts with DNA and the chromosome in a way similar to that of hydroxylamine and hydrazines.", "PMID": 958235}
{"id": "pubmed23n0032_3027", "title": "Evaluation of the mutagenic potential of cyclohexylamine on spermatogonia of the Chinese hamster.", "content": "In a cytogenetic study on the spermatogonia of Chinese hamster, cyclohexylamine (neutral sulphate) was evaluated for mutagenic effects in comparison with an untreated control group and a group treated with the mutagenic compound cyclophosphamide, by assessing spermatogonial metaphases of treated Chinese hamster for chromosomal structural changes. Each test group comprised 8 male hamsters selected at random. Approximately 100 metaphases from each animal were assessed. The doses were 5 X 150 mg cyclohexylamine sulphate (approx. 5 X 102 mg base) per kg body-weight orally, and 5 X 100 mg cyclophosphamide per kg body-weight orally. The individual doses were administered at intervals of 24 h. Preparations were made 24 h after the final treatment, essentially by the method of Hoo and Bowles [10]. Gaps, breaks, fragments, deletions and translocations were assessed as structural changes; frequencies were determined of the metaphases (a) with aberration(s) including gaps, (b)with aberration(s) less gaps and (c)with translocation(s). Aberrations occurred in the untreated negative control group (1.24% incl. gaps, 0.25% without gaps). Translocations were not seen in the untreated group. In the cyclochexylamine group, the frequencies of the aberrant metaphases were sometimes less than in the control group (0.87% including gaps, 0.37% without gaps). Statistically, the results were not significantly different from the control data. No translocations were seen after administration of cyclohexylamine. The positive cyclophosphamide control group clearly differed from the untreated control and from the cyclohexylamine group in the parameters (a) to (c); mainly, the results were highly significantly different from those obtained in the untreated control group. The frequencies of the aberrant metaphases were 3.41% including gaps and 1.99% without gaps. The frequency of the translocations was 0.71% (5 out of 704). Cyclohexylamine sulphate, administered 5 times at 150 mg/kg body-weight orally, had no mutagenic effect, whereas cyclophosphamide, adminstered 5 times at 100 mg/kg body-weight orally, had a chromosome-damaging effect on Chinese hamster spermatogonia.", "contents": "Evaluation of the mutagenic potential of cyclohexylamine on spermatogonia of the Chinese hamster. In a cytogenetic study on the spermatogonia of Chinese hamster, cyclohexylamine (neutral sulphate) was evaluated for mutagenic effects in comparison with an untreated control group and a group treated with the mutagenic compound cyclophosphamide, by assessing spermatogonial metaphases of treated Chinese hamster for chromosomal structural changes. Each test group comprised 8 male hamsters selected at random. Approximately 100 metaphases from each animal were assessed. The doses were 5 X 150 mg cyclohexylamine sulphate (approx. 5 X 102 mg base) per kg body-weight orally, and 5 X 100 mg cyclophosphamide per kg body-weight orally. The individual doses were administered at intervals of 24 h. Preparations were made 24 h after the final treatment, essentially by the method of Hoo and Bowles [10]. Gaps, breaks, fragments, deletions and translocations were assessed as structural changes; frequencies were determined of the metaphases (a) with aberration(s) including gaps, (b)with aberration(s) less gaps and (c)with translocation(s). Aberrations occurred in the untreated negative control group (1.24% incl. gaps, 0.25% without gaps). Translocations were not seen in the untreated group. In the cyclochexylamine group, the frequencies of the aberrant metaphases were sometimes less than in the control group (0.87% including gaps, 0.37% without gaps). Statistically, the results were not significantly different from the control data. No translocations were seen after administration of cyclohexylamine. The positive cyclophosphamide control group clearly differed from the untreated control and from the cyclohexylamine group in the parameters (a) to (c); mainly, the results were highly significantly different from those obtained in the untreated control group. The frequencies of the aberrant metaphases were 3.41% including gaps and 1.99% without gaps. The frequency of the translocations was 0.71% (5 out of 704). Cyclohexylamine sulphate, administered 5 times at 150 mg/kg body-weight orally, had no mutagenic effect, whereas cyclophosphamide, adminstered 5 times at 100 mg/kg body-weight orally, had a chromosome-damaging effect on Chinese hamster spermatogonia.", "PMID": 958236}
{"id": "pubmed23n0032_3028", "title": "Further studies on the histological cytological and cytogenetic effects of nogalamycin in mammals.", "content": "The histological, cytological and cytogenetic effects of nogalamycin were studied in rats, mice and cultured human leukocytes. Four standard test systems were used in the cytological studies: (1)analysis of spermatogonial cells, (2)cytological evaluation of bone marrow metaphase plates, (3)micronucleus test in polychromatic erythrocytes, and (4)determination of chromosomal aberrations in cultured human leukocytes. The results of the studies indicated that the types and frequenices of cytological errors induced by the drug were not significantly different from the concurrent control. The reduced incidence of fertile matings from 22 to 35 days after treatment is attributed to the cytostatic effect of nogalamycin (NM) on spermatogonia and spermatocytes. Subsidence of NM-induced inhibition of spermatogonium differentiation and spermatocyte maturation occurred 37 days post-treatment. Accordingly, fertile matings, which had reached a zero level by 23rd day, occurred 35 days after treatment, and reached the control level between 47 and 49 days post-treatment.", "contents": "Further studies on the histological cytological and cytogenetic effects of nogalamycin in mammals. The histological, cytological and cytogenetic effects of nogalamycin were studied in rats, mice and cultured human leukocytes. Four standard test systems were used in the cytological studies: (1)analysis of spermatogonial cells, (2)cytological evaluation of bone marrow metaphase plates, (3)micronucleus test in polychromatic erythrocytes, and (4)determination of chromosomal aberrations in cultured human leukocytes. The results of the studies indicated that the types and frequenices of cytological errors induced by the drug were not significantly different from the concurrent control. The reduced incidence of fertile matings from 22 to 35 days after treatment is attributed to the cytostatic effect of nogalamycin (NM) on spermatogonia and spermatocytes. Subsidence of NM-induced inhibition of spermatogonium differentiation and spermatocyte maturation occurred 37 days post-treatment. Accordingly, fertile matings, which had reached a zero level by 23rd day, occurred 35 days after treatment, and reached the control level between 47 and 49 days post-treatment.", "PMID": 958237}
{"id": "pubmed23n0032_3029", "title": "Effect of tetracycline on cultured mouse cells.", "content": "By use of an assay system in vitro with cultured FM3A cells from a C3H mouse mammary carcinoma, tetracycline hydrochloride was found to have the ability to induce an 8-azaguanine-resistant mutation. It is suggested that an assay system using mammalian cells for the detection of mutagenicity of certain substances having antimicrobial action is more practical than a system using bacteria. As other effects of tetracycline hydrochloride on FM3A cells, the damage on chromosomes and the inhibition of syntheses of protein and nucleic acids were demonstrated.", "contents": "Effect of tetracycline on cultured mouse cells. By use of an assay system in vitro with cultured FM3A cells from a C3H mouse mammary carcinoma, tetracycline hydrochloride was found to have the ability to induce an 8-azaguanine-resistant mutation. It is suggested that an assay system using mammalian cells for the detection of mutagenicity of certain substances having antimicrobial action is more practical than a system using bacteria. As other effects of tetracycline hydrochloride on FM3A cells, the damage on chromosomes and the inhibition of syntheses of protein and nucleic acids were demonstrated.", "PMID": 958238}
{"id": "pubmed23n0032_3030", "title": "Immunoglobulin content in the bronchial washings of patients with benign and malignant pulmonary disease.", "content": "The bronchial washings of 58 patients with benign and neoplastic conditions involving the lungs were evaluated for immunoglobulin content. Levels in the washings from the diseased lung were compared with those from the normal contralateral lung. In normal patients and those with either bilateral inflammatory diseases or unilateral bronchiectasis or tuberculosis, the mean variation in igA/K and igG/K ratios between diseased and uninvolved lungs was minimal. In unilateral pneumonitis, however, igG/K was markedly elevated (P less than 0.01). Significant changes in the igA/K occurred on the affected side in patients with cancer. In the group with squamous-cell cancer. the mean elevation was 4.6 times that of contralateral lung (P less than 0.005), whereas with adenocarcinoma and undifferentiated carcinoma, the rise was 6.4 and 2.8 times, respecitvely (P less than 0.01 and P less than 0.01). Thus, carcinoma appears to alter local immunoglobulin production in the affected lung as compared to its normal counterpart. (N Engl J Med 295:694-698, 1976).", "contents": "Immunoglobulin content in the bronchial washings of patients with benign and malignant pulmonary disease. The bronchial washings of 58 patients with benign and neoplastic conditions involving the lungs were evaluated for immunoglobulin content. Levels in the washings from the diseased lung were compared with those from the normal contralateral lung. In normal patients and those with either bilateral inflammatory diseases or unilateral bronchiectasis or tuberculosis, the mean variation in igA/K and igG/K ratios between diseased and uninvolved lungs was minimal. In unilateral pneumonitis, however, igG/K was markedly elevated (P less than 0.01). Significant changes in the igA/K occurred on the affected side in patients with cancer. In the group with squamous-cell cancer. the mean elevation was 4.6 times that of contralateral lung (P less than 0.005), whereas with adenocarcinoma and undifferentiated carcinoma, the rise was 6.4 and 2.8 times, respecitvely (P less than 0.01 and P less than 0.01). Thus, carcinoma appears to alter local immunoglobulin production in the affected lung as compared to its normal counterpart. (N Engl J Med 295:694-698, 1976).", "PMID": 958243}
{"id": "pubmed23n0032_3031", "title": "Implementing formative health planning under PL 93-641.", "content": "The National Health Planning and Resources Development Act of 1974 (PL 93-641) melds the Hill-Burton program, Regional Medical Program and Comprehensive Health Planning into a new network for health planning and resources development. Health-systems agencies will possess broader powers than predecessor agencies, particularly in the areas of regulation, control of federal funds, resources development and implementation. PL 93-641 thus offers the possibility of transforming the basic concept of health planning from reactive to \"proactive.\" Successful legislative implementaion will require each health-systems agency to build local legitimacy, ensure constructive consumer/provider dialogue, and respond to state and national managerial requirements. Many questions about planning implementation, the role of subarea councils, agency co-ordination and local governance remain unresolved. The new health-planning network has the potential to assume the function of active system transformation, but will be critically dependent on adequate program budgeting to fulfill this promise. (N Engl J Med 295:698-703, 1976).", "contents": "Implementing formative health planning under PL 93-641. The National Health Planning and Resources Development Act of 1974 (PL 93-641) melds the Hill-Burton program, Regional Medical Program and Comprehensive Health Planning into a new network for health planning and resources development. Health-systems agencies will possess broader powers than predecessor agencies, particularly in the areas of regulation, control of federal funds, resources development and implementation. PL 93-641 thus offers the possibility of transforming the basic concept of health planning from reactive to \"proactive.\" Successful legislative implementaion will require each health-systems agency to build local legitimacy, ensure constructive consumer/provider dialogue, and respond to state and national managerial requirements. Many questions about planning implementation, the role of subarea councils, agency co-ordination and local governance remain unresolved. The new health-planning network has the potential to assume the function of active system transformation, but will be critically dependent on adequate program budgeting to fulfill this promise. (N Engl J Med 295:698-703, 1976).", "PMID": 958244}
{"id": "pubmed23n0032_3032", "title": "Treatment of idiopathic membranous nephropathy.", "content": "In a retrospective study of the effect of treatment in biopsy-proved idiopathic membranous nephropathy, 91 adults and 12 children were followed for periods up to 29 years after clinical onset (mean, 6.5 years). Forty-four were treated with corticosteroids, 15 with corticosteroids and immunosuppressants; 44 had no treatment and served as a control group. Clinical cure and improvement were significantly greater in the treated than in the nontreated group (P less than 0.01). The recurrence rate, occurrence of renal failure and probability of death were significantly greater in the nontreated group, although some of these patients eventually showed improvement. Prognosis was better in patients who responded to therapy. These data strongly suggest that steroid therapy is beneficial in patients with membranous nephropathy.", "contents": "Treatment of idiopathic membranous nephropathy. In a retrospective study of the effect of treatment in biopsy-proved idiopathic membranous nephropathy, 91 adults and 12 children were followed for periods up to 29 years after clinical onset (mean, 6.5 years). Forty-four were treated with corticosteroids, 15 with corticosteroids and immunosuppressants; 44 had no treatment and served as a control group. Clinical cure and improvement were significantly greater in the treated than in the nontreated group (P less than 0.01). The recurrence rate, occurrence of renal failure and probability of death were significantly greater in the nontreated group, although some of these patients eventually showed improvement. Prognosis was better in patients who responded to therapy. These data strongly suggest that steroid therapy is beneficial in patients with membranous nephropathy.", "PMID": 958260}
{"id": "pubmed23n0032_3033", "title": "Chlorambucil treatment of frequently relapsing nephrotic syndrome.", "content": "Chlorambucil, in combination with prednisone, was compared with prednisone alone in a randomized controlled trial in 21 children with either steroid-dependent or frequently relapsing nephrotic syndrome to assess its effect on the duration of remission and the rate of relapse. All control patients treated with prednisone alone continued to relapse at the same rate, with all patients experiencing a return of proteinuria by seven months. Conversely, those who received the same prednisone therapy along with chlorambucil for six to 12 weeks remained in complete remission, without further medication, during 12 to 34 months of follow-up observation. Complications were minimal. Immediate side effects commonly reported with cyclophosphamide were not seen with chlorambucil. Comparison with published reports also suggests that remission induced by chlorambucil is more stable than that after cyclophosphamide. Chlorambucil appears to be of value in the frequently relapsing nephrotic patient, adding an effect that is unattainable with prednisone alone.", "contents": "Chlorambucil treatment of frequently relapsing nephrotic syndrome. Chlorambucil, in combination with prednisone, was compared with prednisone alone in a randomized controlled trial in 21 children with either steroid-dependent or frequently relapsing nephrotic syndrome to assess its effect on the duration of remission and the rate of relapse. All control patients treated with prednisone alone continued to relapse at the same rate, with all patients experiencing a return of proteinuria by seven months. Conversely, those who received the same prednisone therapy along with chlorambucil for six to 12 weeks remained in complete remission, without further medication, during 12 to 34 months of follow-up observation. Complications were minimal. Immediate side effects commonly reported with cyclophosphamide were not seen with chlorambucil. Comparison with published reports also suggests that remission induced by chlorambucil is more stable than that after cyclophosphamide. Chlorambucil appears to be of value in the frequently relapsing nephrotic patient, adding an effect that is unattainable with prednisone alone.", "PMID": 958261}
{"id": "pubmed23n0032_3034", "title": "Effect of alcohol (ethanol) administration on sex-hormone metabolism in normal men.", "content": "To determine whether ethanol per se affects testosterone metabolism, alcohol was administered to normal male volunteers for periods up to four weeks, resulting in an initial dampening of the episodic bursts of testosterone secretion followed by decreases in both the mean plasma concentration and the production rate of testosterone. The volunteers received adequate nutrition and none lost weight during the study, which tended to exclude a nutritional disturbance as the cause of the decreased testosterone levels. The changes in plasma luteinizing hormone suggested both a central (hypothalamus-pituitary) and gonadal effect of alcohol. In addition, alcohol consumption increased the metabolic clearance rate of testosterone in most subjects studied, probably owing to the combined effects of a decreased plasma binding capacity for the androgen and increased hepatic testosterone A-ring reductase activity. These results indicate that alcohol markedly affects testosterone metabolism independently of cirrhosis or nutritional factors.", "contents": "Effect of alcohol (ethanol) administration on sex-hormone metabolism in normal men. To determine whether ethanol per se affects testosterone metabolism, alcohol was administered to normal male volunteers for periods up to four weeks, resulting in an initial dampening of the episodic bursts of testosterone secretion followed by decreases in both the mean plasma concentration and the production rate of testosterone. The volunteers received adequate nutrition and none lost weight during the study, which tended to exclude a nutritional disturbance as the cause of the decreased testosterone levels. The changes in plasma luteinizing hormone suggested both a central (hypothalamus-pituitary) and gonadal effect of alcohol. In addition, alcohol consumption increased the metabolic clearance rate of testosterone in most subjects studied, probably owing to the combined effects of a decreased plasma binding capacity for the androgen and increased hepatic testosterone A-ring reductase activity. These results indicate that alcohol markedly affects testosterone metabolism independently of cirrhosis or nutritional factors.", "PMID": 958274}
{"id": "pubmed23n0032_3035", "title": "Septicemia and meningitis in children splenectomized for hodgkin's disease.", "content": "Retrospective evaluation of the occurrence of septicemia and meningitis in 200 children who had staging laparotomy iwth splenectomy for Hodgkin's disease revealed 20 episodes occurring in 18 children. Symptoms were usually fulminant; only 10 of these patients survived their episode. Infections occurred eight days to three years after splenectomy. Adolescents, as well as younger children, were affected; half were older than 10 years of age. Leukopenia was not a major factor in onset or survival since the average white-cell count was 12,000 in both survivors and children who died. Pneumonococcus accounted for 50 per cent, and streptococcus for 15 per cent of infections; there was one episode each of Haemophilus influenzae and meningococcus; in 25 per cent, no organism was isolated. Predominance of penicillin-sensitive organisms and high mortality suggest that penicillin prophylaxis and the protection offered by bacterial vaccines should be evaluated in children with Hodgkin's disease whose staging laparotomy includes splenectomy.", "contents": "Septicemia and meningitis in children splenectomized for hodgkin's disease. Retrospective evaluation of the occurrence of septicemia and meningitis in 200 children who had staging laparotomy iwth splenectomy for Hodgkin's disease revealed 20 episodes occurring in 18 children. Symptoms were usually fulminant; only 10 of these patients survived their episode. Infections occurred eight days to three years after splenectomy. Adolescents, as well as younger children, were affected; half were older than 10 years of age. Leukopenia was not a major factor in onset or survival since the average white-cell count was 12,000 in both survivors and children who died. Pneumonococcus accounted for 50 per cent, and streptococcus for 15 per cent of infections; there was one episode each of Haemophilus influenzae and meningococcus; in 25 per cent, no organism was isolated. Predominance of penicillin-sensitive organisms and high mortality suggest that penicillin prophylaxis and the protection offered by bacterial vaccines should be evaluated in children with Hodgkin's disease whose staging laparotomy includes splenectomy.", "PMID": 958275}
{"id": "pubmed23n0032_3036", "title": "Intrahepatic cholestasis in childhood.", "content": "The apparent well-being of some children who as neonates were believed to have obstructive jaundice prompted us to study the clinical course, histologic features and possible etiologic factors in 17 children with cholestasis in the neonatal period. During a follow-up period of five months to 22 years, all had signs of chronic cholestasis, but only four died (two from nonhepatic causes); the others live remarkably normal lives. Serial hepatic biopsies in 11 showed a variety of initial lesions, which progressed to hypoplasia of the intrahepatic bile ducts, increasing portal fibrosis and eventual cirrhosis. Although evidence of possible viral infection was found in only 10 cases, a hepatitis, beginning either before or after birth, appears to be a likely original cause. The histologic changes seen may represent different stages of one process, starting as cholestasis with or without evidence of hepatitis and progressing to obliteration or failure of normal growth of the intrahepatic bile ducts.", "contents": "Intrahepatic cholestasis in childhood. The apparent well-being of some children who as neonates were believed to have obstructive jaundice prompted us to study the clinical course, histologic features and possible etiologic factors in 17 children with cholestasis in the neonatal period. During a follow-up period of five months to 22 years, all had signs of chronic cholestasis, but only four died (two from nonhepatic causes); the others live remarkably normal lives. Serial hepatic biopsies in 11 showed a variety of initial lesions, which progressed to hypoplasia of the intrahepatic bile ducts, increasing portal fibrosis and eventual cirrhosis. Although evidence of possible viral infection was found in only 10 cases, a hepatitis, beginning either before or after birth, appears to be a likely original cause. The histologic changes seen may represent different stages of one process, starting as cholestasis with or without evidence of hepatitis and progressing to obliteration or failure of normal growth of the intrahepatic bile ducts.", "PMID": 958276}
{"id": "pubmed23n0032_3037", "title": "Platelet coagulant activities and serum lipids in transient cerebral ischemia.", "content": "To determine whether platelets play a part in the pathogenesis of transient cerebrovascular ischemia, we studied 22 patients with transient ischemia, 18 control patients and 38 normal subjects. Platelet aggregation and [14C]-serotonin release by ADP, epinephrine and collagen were normal in all patients, as were plasma coagulation assays, except for shortened partial thromboplastin times in the patients with transient ischemia. Platelet coagulant activities concerned with initiation and early stages of intrinsic coagulation were increased two to three times in 12 patients with transient ischemic attacks with normal serum lipids and normal in the 10 others with Type IV hyperlipoproteinemia. These results indicate an association between platelet coagulant hyperactivity and transient ischemic attacks in a group of patients with normal serum lipids.", "contents": "Platelet coagulant activities and serum lipids in transient cerebral ischemia. To determine whether platelets play a part in the pathogenesis of transient cerebrovascular ischemia, we studied 22 patients with transient ischemia, 18 control patients and 38 normal subjects. Platelet aggregation and [14C]-serotonin release by ADP, epinephrine and collagen were normal in all patients, as were plasma coagulation assays, except for shortened partial thromboplastin times in the patients with transient ischemia. Platelet coagulant activities concerned with initiation and early stages of intrinsic coagulation were increased two to three times in 12 patients with transient ischemic attacks with normal serum lipids and normal in the 10 others with Type IV hyperlipoproteinemia. These results indicate an association between platelet coagulant hyperactivity and transient ischemic attacks in a group of patients with normal serum lipids.", "PMID": 958287}
{"id": "pubmed23n0032_3038", "title": "Gender of offspring after oral-contraceptive use.", "content": "We examined the sex of offspring born after oral-contraceptive use according to duration of contraceptive use and the interval between cessation of use and conception for 6109 live births. The overall proportion of male births (with 90 per cent confidence) falls between 0.491 and 0.512. These data exclude with high probability a shift in the sex distribution of the magnitude previously hypothesized, and are not consistent with even a moderate shift in sex distribution. Sex of offspring was not related to total duration of oral-contraceptive use, duration of use since any previous pregnancy or interval between termination of use and conception. It appears that oral contraceptives have no bearing on the sex of subsequent offspring.", "contents": "Gender of offspring after oral-contraceptive use. We examined the sex of offspring born after oral-contraceptive use according to duration of contraceptive use and the interval between cessation of use and conception for 6109 live births. The overall proportion of male births (with 90 per cent confidence) falls between 0.491 and 0.512. These data exclude with high probability a shift in the sex distribution of the magnitude previously hypothesized, and are not consistent with even a moderate shift in sex distribution. Sex of offspring was not related to total duration of oral-contraceptive use, duration of use since any previous pregnancy or interval between termination of use and conception. It appears that oral contraceptives have no bearing on the sex of subsequent offspring.", "PMID": 958288}
{"id": "pubmed23n0032_3039", "title": "Respiratory failure associated with familial depression of ventilatory response to hypoxia and hypercapnia.", "content": "Respiratory failure has been associated with depressed ventilatory responses to hypoxia or hypercapnia or both. The possibility that familial factors are responsible for decreased chemosensitivity prompted this study of a child with unexplained respiratory failure and normal lung function. We found his ventilatory response to hypoxia and hypercapnia to be virtually absent. Studies of six healthy immediate family members (parents and siblings) showed that hypoxic response, as measured by an index of the relation between ventilation and hypoxia (index A), was consistently reduced: 45 +/- 8.7 S.E.M. (normal, 127 +/- 8.7) (P less than 0.005). Response to hypercapnia, measured as the slope of the ventilatory response to hypercapnia, was lower than normal, averaging 0.95 +/- 0.16 liters per minute per millimeter of mercury (normal, 1.76 +/- 0.13) (P less than 0.01). The patient's respiratory failure seemed related to deficient ventilatory responses to hypoxia and hypercapnia. It seems likely that this depressed hypoxic response is of familial origin.", "contents": "Respiratory failure associated with familial depression of ventilatory response to hypoxia and hypercapnia. Respiratory failure has been associated with depressed ventilatory responses to hypoxia or hypercapnia or both. The possibility that familial factors are responsible for decreased chemosensitivity prompted this study of a child with unexplained respiratory failure and normal lung function. We found his ventilatory response to hypoxia and hypercapnia to be virtually absent. Studies of six healthy immediate family members (parents and siblings) showed that hypoxic response, as measured by an index of the relation between ventilation and hypoxia (index A), was consistently reduced: 45 +/- 8.7 S.E.M. (normal, 127 +/- 8.7) (P less than 0.005). Response to hypercapnia, measured as the slope of the ventilatory response to hypercapnia, was lower than normal, averaging 0.95 +/- 0.16 liters per minute per millimeter of mercury (normal, 1.76 +/- 0.13) (P less than 0.01). The patient's respiratory failure seemed related to deficient ventilatory responses to hypoxia and hypercapnia. It seems likely that this depressed hypoxic response is of familial origin.", "PMID": 958289}
{"id": "pubmed23n0032_3040", "title": "Strategies for financing national health insurance: who wins and who loses.", "content": "Two sources of funds are available to underwrite the costs of any national health-insurance plan: prepayments (premiums, payroll taxes and income taxes) and out-of-pocket payments (coinsurance and deductibles). The extent to which taxes rather than premiums are used to finance an insurance program will be the major determinant of how large a share of the costs of health care will be borne by higher-income groups. The extent to which coinsurance and deductible provisions are reduced or waived for low-income persons will have a less important, but still substantial, role in determining how the costs of a program are distributed. These financing principles, once understood, provide a basis for the design of health-insurance legislation that will achieve any pattern of income redistribution that may be desired.", "contents": "Strategies for financing national health insurance: who wins and who loses. Two sources of funds are available to underwrite the costs of any national health-insurance plan: prepayments (premiums, payroll taxes and income taxes) and out-of-pocket payments (coinsurance and deductibles). The extent to which taxes rather than premiums are used to finance an insurance program will be the major determinant of how large a share of the costs of health care will be borne by higher-income groups. The extent to which coinsurance and deductible provisions are reduced or waived for low-income persons will have a less important, but still substantial, role in determining how the costs of a program are distributed. These financing principles, once understood, provide a basis for the design of health-insurance legislation that will achieve any pattern of income redistribution that may be desired.", "PMID": 958290}
{"id": "pubmed23n0032_3041", "title": "Lipid oxidation. Part. 1. Effect of free carboxyl group on the decomposition of lipid hydroperoxide.", "content": "Hydroperoxido butyl oleate was decomposed by heating in excess palmitic acid at 60-120 degrees C. The decomposition followed the kinetics of a first order reaction with formation of both monomeric and oligomeric secondary products. The proportions of oligomers slightly increased with increasing reaction temperature and decreased with increasing concentration of hydroperoxide. The activation energy was 70.4 kJ/mol +/- 4.7 kJ/mol. The decomposition of hydroperoxides proceeded partially by monomolecular cleavage, partially by formation of esters with palmitic acid.", "contents": "Lipid oxidation. Part. 1. Effect of free carboxyl group on the decomposition of lipid hydroperoxide. Hydroperoxido butyl oleate was decomposed by heating in excess palmitic acid at 60-120 degrees C. The decomposition followed the kinetics of a first order reaction with formation of both monomeric and oligomeric secondary products. The proportions of oligomers slightly increased with increasing reaction temperature and decreased with increasing concentration of hydroperoxide. The activation energy was 70.4 kJ/mol +/- 4.7 kJ/mol. The decomposition of hydroperoxides proceeded partially by monomolecular cleavage, partially by formation of esters with palmitic acid.", "PMID": 958305}
{"id": "pubmed23n0032_3042", "title": "[Spinning of rapeseed proteins].", "content": "Alkaline solutions of a mixture from rapeseed globulin and casein show plastic flow, which is influenced by protein concentration, relation of rapeseed globulin and casein, temperature and time of storage of the protein solution. The sodium hydroxide concentration with a great variability doesn't take effect on the rheological properties. With increasing content of casein in such alkaline solutions of rapeseed globuline and casein results an approach to NEWTONian flow. The spinnability of alkaline solutions of a mixture from rapeseed globulin and casein and the properties of the corresponding spun protein fibers are influenced by protein concentration, relation of rapeseed globulin and casein of the spinning solution and temperature of coagulating bath. The sodium hydroxide content of the spinning solution and the hydrochloric acid concentration of the coagulating bath with a great variability don't take effect. The spinnability of alkaline solutions of a mixture from rapeseed albumin starchsulphate and casein and the properties of the corresponding spun protein fibers compared to rapeseed globulin-casein mixtures are influenced additionally by sodium hydroxide concentration of the spinning solution and hydrochloric acid content of coagulating bath. The favourable influence on the properties of spun rapeseed albuminstarchsulphate-casein fibers isn't increased by a growth of the rapeseed albumin starchsulphate content more then 4% of the protein concentration of the spinning solution.", "contents": "[Spinning of rapeseed proteins]. Alkaline solutions of a mixture from rapeseed globulin and casein show plastic flow, which is influenced by protein concentration, relation of rapeseed globulin and casein, temperature and time of storage of the protein solution. The sodium hydroxide concentration with a great variability doesn't take effect on the rheological properties. With increasing content of casein in such alkaline solutions of rapeseed globuline and casein results an approach to NEWTONian flow. The spinnability of alkaline solutions of a mixture from rapeseed globulin and casein and the properties of the corresponding spun protein fibers are influenced by protein concentration, relation of rapeseed globulin and casein of the spinning solution and temperature of coagulating bath. The sodium hydroxide content of the spinning solution and the hydrochloric acid concentration of the coagulating bath with a great variability don't take effect. The spinnability of alkaline solutions of a mixture from rapeseed albumin starchsulphate and casein and the properties of the corresponding spun protein fibers compared to rapeseed globulin-casein mixtures are influenced additionally by sodium hydroxide concentration of the spinning solution and hydrochloric acid content of coagulating bath. The favourable influence on the properties of spun rapeseed albuminstarchsulphate-casein fibers isn't increased by a growth of the rapeseed albumin starchsulphate content more then 4% of the protein concentration of the spinning solution.", "PMID": 958306}
{"id": "pubmed23n0032_3043", "title": "[Effect of starchsulfate on properties of protein fibers].", "content": "Admixture of starchsulphate to an alkaline spinning solution of proteins increases the thermostability of the corresponding spun protein fibers.", "contents": "[Effect of starchsulfate on properties of protein fibers]. Admixture of starchsulphate to an alkaline spinning solution of proteins increases the thermostability of the corresponding spun protein fibers.", "PMID": 958307}
{"id": "pubmed23n0032_3044", "title": "[Formation of dichlorvos residues on cereal products].", "content": "Dichlorvos should be used in mill rooms where milling products are stored. The contamination of foods stored in such rooms strongly depends on the mode of application, the concentration of the agent and the temperature. The authors deal with the extent of contamination under various conditions and with the degradation rate in contaminated flours.", "contents": "[Formation of dichlorvos residues on cereal products]. Dichlorvos should be used in mill rooms where milling products are stored. The contamination of foods stored in such rooms strongly depends on the mode of application, the concentration of the agent and the temperature. The authors deal with the extent of contamination under various conditions and with the degradation rate in contaminated flours.", "PMID": 958308}
{"id": "pubmed23n0032_3045", "title": "[Field test for determination of mercury in cereals and cereal products].", "content": "By means of the method described, rapid semiquantitative mercury determinations may be performed without energy supply in cereals and cereal products. The mercury ions which are liberated by digestion with concentrated H2SO4 and KMnO4 are determined with the aid of a colorimetric method which is based on the formation of a coloured complex of mercury with di-beta-naphthyl-thiocarbazone. The method permits visual comparison of mercury concentrations ranging from 0.1 to 13 mug, revealing concentration differences up to 0,1 mug. The rate of recovery is 76%.", "contents": "[Field test for determination of mercury in cereals and cereal products]. By means of the method described, rapid semiquantitative mercury determinations may be performed without energy supply in cereals and cereal products. The mercury ions which are liberated by digestion with concentrated H2SO4 and KMnO4 are determined with the aid of a colorimetric method which is based on the formation of a coloured complex of mercury with di-beta-naphthyl-thiocarbazone. The method permits visual comparison of mercury concentrations ranging from 0.1 to 13 mug, revealing concentration differences up to 0,1 mug. The rate of recovery is 76%.", "PMID": 958310}
{"id": "pubmed23n0032_3046", "title": "[Vitamin A determination in commercially produced feeds. 2. Vitamin A determination in mixed feeds].", "content": "On the basis of a study of methods for the determination of vitamin A in mixed feeds, it is shown that both the vitamin A determination according to CARR and PRICE and the determination of anhydro-vitamin A yield valid results. With regard to accuracy, easy performance and productivity, the determination of anhydro-vitamin A is the better suited method.", "contents": "[Vitamin A determination in commercially produced feeds. 2. Vitamin A determination in mixed feeds]. On the basis of a study of methods for the determination of vitamin A in mixed feeds, it is shown that both the vitamin A determination according to CARR and PRICE and the determination of anhydro-vitamin A yield valid results. With regard to accuracy, easy performance and productivity, the determination of anhydro-vitamin A is the better suited method.", "PMID": 958312}
{"id": "pubmed23n0032_3047", "title": "[In vitro study on effect of body weight on deiodination of L-diiodotyrosine and L-thyroxine in liver and kidney of rats on standard diets].", "content": "The effect of body weight on the deiodination of L-di-iodotyrosine and L-thyroxin in the liver and kidney was studied in vitro in male Wistar rats of same age, but differing in weight (light and heavy, respectively), which were fed a standard diet. In the livers of the heavy animals, the deiodination of L-di-iodotyrosine and L-thyroxin was significantly lower than in those of the light animals. As to the kidneys, no such difference was observed. From a comparison with literature data it is concluded that the reduction in deiodination in the livers of the heavy animals might be due to a reduction in thyroid hormone metabolism.", "contents": "[In vitro study on effect of body weight on deiodination of L-diiodotyrosine and L-thyroxine in liver and kidney of rats on standard diets]. The effect of body weight on the deiodination of L-di-iodotyrosine and L-thyroxin in the liver and kidney was studied in vitro in male Wistar rats of same age, but differing in weight (light and heavy, respectively), which were fed a standard diet. In the livers of the heavy animals, the deiodination of L-di-iodotyrosine and L-thyroxin was significantly lower than in those of the light animals. As to the kidneys, no such difference was observed. From a comparison with literature data it is concluded that the reduction in deiodination in the livers of the heavy animals might be due to a reduction in thyroid hormone metabolism.", "PMID": 958320}
{"id": "pubmed23n0032_3048", "title": "[In vitro study of deiodination of L-diiodotyrosine and L-thyroxine in liver of dietetically fat rats].", "content": "Beginning with the fifth week of life, male Wistar rats were fed a diet containing, respectively, 3 and 50 per cent fat (corresponding to, respectively, 7 and 75 per cent fat of total calories). The differences in body weight between the two groups were 64 per cent for 10-week-old rats, and 71 per cent for 6-month-old rats. For these animals, the extent of the enzymatic deiodination of L-diiodotyrosine and L-thyroxin was determined in liver homogenate supernatants. In the 10-week-old rats as well as in the 6-month-old rats on high-fat diet, the deiodination of L-di-iodotyrosine was significantly lower than in the animals on low-fat diet, whereas the diodination of L-thyroxin was significantly higher. It is concluded that the fat content of the diet exerts an inhibiting effect on the deiodination of L-di-iodotyrosine and an enhancing effect on the deiodination of L-thyroxin.", "contents": "[In vitro study of deiodination of L-diiodotyrosine and L-thyroxine in liver of dietetically fat rats]. Beginning with the fifth week of life, male Wistar rats were fed a diet containing, respectively, 3 and 50 per cent fat (corresponding to, respectively, 7 and 75 per cent fat of total calories). The differences in body weight between the two groups were 64 per cent for 10-week-old rats, and 71 per cent for 6-month-old rats. For these animals, the extent of the enzymatic deiodination of L-diiodotyrosine and L-thyroxin was determined in liver homogenate supernatants. In the 10-week-old rats as well as in the 6-month-old rats on high-fat diet, the deiodination of L-di-iodotyrosine was significantly lower than in the animals on low-fat diet, whereas the diodination of L-thyroxin was significantly higher. It is concluded that the fat content of the diet exerts an inhibiting effect on the deiodination of L-di-iodotyrosine and an enhancing effect on the deiodination of L-thyroxin.", "PMID": 958322}
{"id": "pubmed23n0032_3049", "title": "[Effect of diet on deiodination of L-diiodotyrosine and L-thyroxine in rat liver].", "content": "Male Wistar rats of same weight were used to study the effects of diets containing, respectively, 3 and 50 per cent (by weight) fat on the deiodination of L-di-iodotyrosine and L-thyroxin in the liver. For this purpose, liver homogenate supernatants were analysed. The deiodination of L-di-iodotyrosine was significantly lower in the animals on high-fat diet than in those on low-fat diet, whereas the deiodination of L-thyroxin was significantly higher. It is concluded that not the body weight, but the diet influences the extent of deiodination.", "contents": "[Effect of diet on deiodination of L-diiodotyrosine and L-thyroxine in rat liver]. Male Wistar rats of same weight were used to study the effects of diets containing, respectively, 3 and 50 per cent (by weight) fat on the deiodination of L-di-iodotyrosine and L-thyroxin in the liver. For this purpose, liver homogenate supernatants were analysed. The deiodination of L-di-iodotyrosine was significantly lower in the animals on high-fat diet than in those on low-fat diet, whereas the deiodination of L-thyroxin was significantly higher. It is concluded that not the body weight, but the diet influences the extent of deiodination.", "PMID": 958326}
{"id": "pubmed23n0032_3050", "title": "Lipid oxidation. Part 2. Oxidation products of olive oil methyl esters.", "content": "Olive oil was converted into methyl esters which were autoxidized at 60 degrees C. The composition of oxidized products was determined by the comparison of infrared spectra and NMR spectra of the original and acetylated samples, the sample reduced with potassium iodide and the acetylated reduced sample. Oxidized products were separated by preparative thin layer chromatography on silica gel and characterized by selective detection and by infrared spectrometry of the fractions. The oxidation products consisted of hydroperoxido butyl oleate, substituted hydroperoxides, mono- and disubstituted monomeric derivatives and a small amount of oligomers.", "contents": "Lipid oxidation. Part 2. Oxidation products of olive oil methyl esters. Olive oil was converted into methyl esters which were autoxidized at 60 degrees C. The composition of oxidized products was determined by the comparison of infrared spectra and NMR spectra of the original and acetylated samples, the sample reduced with potassium iodide and the acetylated reduced sample. Oxidized products were separated by preparative thin layer chromatography on silica gel and characterized by selective detection and by infrared spectrometry of the fractions. The oxidation products consisted of hydroperoxido butyl oleate, substituted hydroperoxides, mono- and disubstituted monomeric derivatives and a small amount of oligomers.", "PMID": 958335}
{"id": "pubmed23n0032_3051", "title": "Photometric determination of dithiocarbamate residues by carbon disulphide evaluation method.", "content": "A review of analytical methods for determination of dithiocarbamate residues by carbon disulphide evaluation method was made. On basis of KEPPEL method the colorimetric step was modified. For this purpose the reaction of hydrogen sulphide with p-aminodimethylaniline in the presence of ferric and zinc ions to form methylene blue was applied. The coloured compound after a chloroform extraction was measured at 650 nm. The method gives a very high sensitivity of about 2 mug. Recoveries obtained with zineb and maneb for various crops are satisfactory.", "contents": "Photometric determination of dithiocarbamate residues by carbon disulphide evaluation method. A review of analytical methods for determination of dithiocarbamate residues by carbon disulphide evaluation method was made. On basis of KEPPEL method the colorimetric step was modified. For this purpose the reaction of hydrogen sulphide with p-aminodimethylaniline in the presence of ferric and zinc ions to form methylene blue was applied. The coloured compound after a chloroform extraction was measured at 650 nm. The method gives a very high sensitivity of about 2 mug. Recoveries obtained with zineb and maneb for various crops are satisfactory.", "PMID": 958336}
{"id": "pubmed23n0032_3052", "title": "[Lysosomal enzymes in starvation after regimen of single or all-day feeding].", "content": "The authors studied in rats the effect of starving from 24 to 96 hours after a regimen of single or all-day feeding. They determined in liver homogenate the total activities of beta-N-acetyl glucosaminidase, acid phosphatase and beta-glucuronidase as well as the free activities of the two first-mentioned enzymes. The activity of beta-N-acetyl glucosaminidase was also determined in the blood. It was found that the total activity of beta-N-acetyl glucosaminidase in the liver homogenate increased with the length of starvation. The free and serum activities of the enzymes under investigation showed similar changes. These increases were more marked in rats subjected to a single-feeding regimen before starving. These changes are explained as an expression of the active participation of the lysosomes and their enzymes in the transition to endogenous feeding. The lysosomal membrane was more sensitive in starving animals which had been subjected to a single-feeding regimen.", "contents": "[Lysosomal enzymes in starvation after regimen of single or all-day feeding]. The authors studied in rats the effect of starving from 24 to 96 hours after a regimen of single or all-day feeding. They determined in liver homogenate the total activities of beta-N-acetyl glucosaminidase, acid phosphatase and beta-glucuronidase as well as the free activities of the two first-mentioned enzymes. The activity of beta-N-acetyl glucosaminidase was also determined in the blood. It was found that the total activity of beta-N-acetyl glucosaminidase in the liver homogenate increased with the length of starvation. The free and serum activities of the enzymes under investigation showed similar changes. These increases were more marked in rats subjected to a single-feeding regimen before starving. These changes are explained as an expression of the active participation of the lysosomes and their enzymes in the transition to endogenous feeding. The lysosomal membrane was more sensitive in starving animals which had been subjected to a single-feeding regimen.", "PMID": 958337}
{"id": "pubmed23n0032_3053", "title": "[Meat quality of broiler rabbits. 2. Chemical composition of meat of valuable cut-up parts of New Zealand white rabbits and hybrids of different body weight].", "content": "Studies were carried out on the chemical composition of the meat of valuable cut-up parts in 88 broiler rabbits (51 New Zealand White, 37 hybrids). Muscles of legs and backs differed in nutrient content. The fat content of legs was higher than that of M.long.dorsi, and directly related to increasing carcass weights. As to the varieties mostly insignificant effects on chemical constituents were measured. There were but a few correlations between the analysed qualitative properties.", "contents": "[Meat quality of broiler rabbits. 2. Chemical composition of meat of valuable cut-up parts of New Zealand white rabbits and hybrids of different body weight]. Studies were carried out on the chemical composition of the meat of valuable cut-up parts in 88 broiler rabbits (51 New Zealand White, 37 hybrids). Muscles of legs and backs differed in nutrient content. The fat content of legs was higher than that of M.long.dorsi, and directly related to increasing carcass weights. As to the varieties mostly insignificant effects on chemical constituents were measured. There were but a few correlations between the analysed qualitative properties.", "PMID": 958338}
{"id": "pubmed23n0032_3054", "title": "[New results on migration behavior of benzophenone-based UV absorbents from polyolefins in foods].", "content": "The authors deal with the migration behaviour of bezophenone-based ultraviolet absorbents for commodities of low-pressure polyethylene, high-pressure polyethlene, polypropylene and polystyrene. These ultraviolet-absorbent-stabilized plastics (up to 0.5% of ultraviolet absorbent) show a very slight tendency of the light stabilizers towards migration into aqueous, acid or dilute alcoholic foods. Very high migration values are obtained for sunflower oil, Fettsimulans HB 307 or 50% ethanol. The amount of 2-hydroxy-4-octoxybenzophenone which migrates into fat-free foods is only a fraction of the maximum daily dose; a risk to the consumers' health might thus be excluded. In fat-containing foods, the maximum daily dose is also not reached, but the single migration value is higher than the limit value (100 p.p.m.) for total migration. Consequently, the above ultraviolet absorbents should not be used for the ultraviolet stabilization of plastics designed for packing these products. An evaluation of the safety of ultraviolet absorbents of the Dastib type will be possible only if sufficient toxicological data are available.", "contents": "[New results on migration behavior of benzophenone-based UV absorbents from polyolefins in foods]. The authors deal with the migration behaviour of bezophenone-based ultraviolet absorbents for commodities of low-pressure polyethylene, high-pressure polyethlene, polypropylene and polystyrene. These ultraviolet-absorbent-stabilized plastics (up to 0.5% of ultraviolet absorbent) show a very slight tendency of the light stabilizers towards migration into aqueous, acid or dilute alcoholic foods. Very high migration values are obtained for sunflower oil, Fettsimulans HB 307 or 50% ethanol. The amount of 2-hydroxy-4-octoxybenzophenone which migrates into fat-free foods is only a fraction of the maximum daily dose; a risk to the consumers' health might thus be excluded. In fat-containing foods, the maximum daily dose is also not reached, but the single migration value is higher than the limit value (100 p.p.m.) for total migration. Consequently, the above ultraviolet absorbents should not be used for the ultraviolet stabilization of plastics designed for packing these products. An evaluation of the safety of ultraviolet absorbents of the Dastib type will be possible only if sufficient toxicological data are available.", "PMID": 958339}
{"id": "pubmed23n0032_3055", "title": "[Rheology and spinning of alkaline solutions of sunflower seed globulin and casein].", "content": "The specific demand of sodium hydroxide is determined for the dissolution of sunflower seed globulin, casein and a mixture of them to equal parts. In low protein-containing solutions it depends for sunflower seed globulin very much on the sodium chloride concentration. From sunflower seed globulin, casein and a mixture of them to equal parts are prepared with sodium hydroxide high protein-containing alkaline solutions. Sunflower seed globulin forms temporally a gel phase. After this phase the solution of sunflower seed globulin shows like casein and a mixture of sunflower seed globulin/casein (I:I) pseudoplastic flow. The flow curves of the pseudoplastic solutions are described mathematically with the OSWALDian power statement. By alkaline solutions of casein and sunflower seed globulin/casein (I:I) the flow exponent n is distributed statistically about 0.9, by solutions of sunflower seed globulin a distribution exists about the mean values n = 0.85 and n = 0.50. lg k depends in all protein solutions on the concentration of protein, sodium chloride, sodium hydroxide and on the temperature and time. For all protein solutions exists a linear relation between the logarithm of viscosity and the reciprocal temperature for lg k and I/T, which is derived normally for NEWTONian flow behaviour. In a suitable scope of spinning for all protein solutions are carried out complete factorial experiment, which guide to regression equations of lg k; in the case of sunflower seed globulin are calculated also a regression equation of the flow exponent n. Going out from the parameters of the spinning process the properties of the spun sunflower seed globulin/casein (I:I) fibers are described.", "contents": "[Rheology and spinning of alkaline solutions of sunflower seed globulin and casein]. The specific demand of sodium hydroxide is determined for the dissolution of sunflower seed globulin, casein and a mixture of them to equal parts. In low protein-containing solutions it depends for sunflower seed globulin very much on the sodium chloride concentration. From sunflower seed globulin, casein and a mixture of them to equal parts are prepared with sodium hydroxide high protein-containing alkaline solutions. Sunflower seed globulin forms temporally a gel phase. After this phase the solution of sunflower seed globulin shows like casein and a mixture of sunflower seed globulin/casein (I:I) pseudoplastic flow. The flow curves of the pseudoplastic solutions are described mathematically with the OSWALDian power statement. By alkaline solutions of casein and sunflower seed globulin/casein (I:I) the flow exponent n is distributed statistically about 0.9, by solutions of sunflower seed globulin a distribution exists about the mean values n = 0.85 and n = 0.50. lg k depends in all protein solutions on the concentration of protein, sodium chloride, sodium hydroxide and on the temperature and time. For all protein solutions exists a linear relation between the logarithm of viscosity and the reciprocal temperature for lg k and I/T, which is derived normally for NEWTONian flow behaviour. In a suitable scope of spinning for all protein solutions are carried out complete factorial experiment, which guide to regression equations of lg k; in the case of sunflower seed globulin are calculated also a regression equation of the flow exponent n. Going out from the parameters of the spinning process the properties of the spun sunflower seed globulin/casein (I:I) fibers are described.", "PMID": 958340}
{"id": "pubmed23n0032_3056", "title": "Volatile sulfur compounds contributing to meat flavour. Part. I. Components identified in boiled meat.", "content": "Volatile sulfur compounds from 3 samples of boiled meat (Longissimus dorsi and shoulder meat) have been isolated, separated from the whole flavour complex by mercuric chloride precipitation, regenerated, and examined by gas chromatography. Identification has been carried through by determining Kovats-indices on 4 gas chromatographic columns of different polarity. 29 sulfur compounds have been identified with high certainty, 10 tentitatively. Among 17 thiols, 8 sulfides, 7 disulfides and trisulfides, and 7 heterocyclic compounds only 11 substances have been described previously in literature. The number of mercaptans especially exceeds hitherto knowledge.", "contents": "Volatile sulfur compounds contributing to meat flavour. Part. I. Components identified in boiled meat. Volatile sulfur compounds from 3 samples of boiled meat (Longissimus dorsi and shoulder meat) have been isolated, separated from the whole flavour complex by mercuric chloride precipitation, regenerated, and examined by gas chromatography. Identification has been carried through by determining Kovats-indices on 4 gas chromatographic columns of different polarity. 29 sulfur compounds have been identified with high certainty, 10 tentitatively. Among 17 thiols, 8 sulfides, 7 disulfides and trisulfides, and 7 heterocyclic compounds only 11 substances have been described previously in literature. The number of mercaptans especially exceeds hitherto knowledge.", "PMID": 958341}
{"id": "pubmed23n0032_3057", "title": "Nutrition and flavour-legislation.", "content": "Our food laws have to provide for safety of food and to ensure that the consumer is not misled. We are no longer in a position to manufacture food without additives and especially without flavours. For physiological reasons, our food should be offered in a wide variety; an increasing number of food preparations has no natural flavour at all and needs to be flavoured and the industrial manufacture of food requires the flavours to be properly developed in order to withstand the sometimes rather drastic conditions of manufacture. The safety of flavours and flavouring substances is discussed. It is shown that the class of nature-identical flavouring substances has to be treated separately for practical purposes of food control and because the substances of this group offer more certainty with regard to their safety. Some suggestions are made regarding the proper labelling of food which contains flavours. The IOFI (International Organization of the Flavour Industry) model law is discussed which should serve as a basic proposal for the international harmonisation of national food laws.", "contents": "Nutrition and flavour-legislation. Our food laws have to provide for safety of food and to ensure that the consumer is not misled. We are no longer in a position to manufacture food without additives and especially without flavours. For physiological reasons, our food should be offered in a wide variety; an increasing number of food preparations has no natural flavour at all and needs to be flavoured and the industrial manufacture of food requires the flavours to be properly developed in order to withstand the sometimes rather drastic conditions of manufacture. The safety of flavours and flavouring substances is discussed. It is shown that the class of nature-identical flavouring substances has to be treated separately for practical purposes of food control and because the substances of this group offer more certainty with regard to their safety. Some suggestions are made regarding the proper labelling of food which contains flavours. The IOFI (International Organization of the Flavour Industry) model law is discussed which should serve as a basic proposal for the international harmonisation of national food laws.", "PMID": 958342}
{"id": "pubmed23n0032_3058", "title": "Changes of sensory value by interaction of alkanals with amino acids and proteins.", "content": "Alkanals as carriers of rancid flavour of fat-containing foods were stored in mixtures with nonlipidic substances. The intensity of odour due to alkanals decreased with increasing time of storage, the changes being more pronounced in case of casein than in that of cellulose. Results of sensory tests did not depend on the molecular mass of aldehyde. The water content affected the sensory evaluation only slightly. The formation of aldolization products or polymers and of gluey flavour compounds modified the character of flavour and confused less experienced judges. Both the binding of alkanals and the aldolization reactions were enhanced by presence of primary amino groups.", "contents": "Changes of sensory value by interaction of alkanals with amino acids and proteins. Alkanals as carriers of rancid flavour of fat-containing foods were stored in mixtures with nonlipidic substances. The intensity of odour due to alkanals decreased with increasing time of storage, the changes being more pronounced in case of casein than in that of cellulose. Results of sensory tests did not depend on the molecular mass of aldehyde. The water content affected the sensory evaluation only slightly. The formation of aldolization products or polymers and of gluey flavour compounds modified the character of flavour and confused less experienced judges. Both the binding of alkanals and the aldolization reactions were enhanced by presence of primary amino groups.", "PMID": 958343}
{"id": "pubmed23n0032_3059", "title": "[Development of fish flavor by reaction of autoxidized lipids with amino acids and proteins].", "content": "The rancid fish-oil flavour of autoxidized tetraene, pentaene and hexaene fatty acid esters disappears during storage or heating with free amino acids or proteins. Simultaneously, a flavour of raw, roasted or fried fish develops. The development of this fish flavour is of particular intensity in mixtures containing lysine or some other amino acids. Blocking of the free amino groups in casein or albumin inhibits the development of a fish flavour in mixtures with oxidized lipids. If the mixtures are added with lysine, the fish flavour reappears. The development of a fish flavour is associated with browning reactions.", "contents": "[Development of fish flavor by reaction of autoxidized lipids with amino acids and proteins]. The rancid fish-oil flavour of autoxidized tetraene, pentaene and hexaene fatty acid esters disappears during storage or heating with free amino acids or proteins. Simultaneously, a flavour of raw, roasted or fried fish develops. The development of this fish flavour is of particular intensity in mixtures containing lysine or some other amino acids. Blocking of the free amino groups in casein or albumin inhibits the development of a fish flavour in mixtures with oxidized lipids. If the mixtures are added with lysine, the fish flavour reappears. The development of a fish flavour is associated with browning reactions.", "PMID": 958344}
{"id": "pubmed23n0032_3060", "title": "[Notes about molecular weights of aroma compounds].", "content": "Classification of 2000 food aroma compounds as to their molecular weight shows an accumulation in the range of 135 to 155, and an upper limit of 310. Molecular masses of substances with high aroma effectiveness are below 200. As aroma compounds are composed only of the elements C, H, O, N and S, even numbers of molecular masses predominate among these substances. A periodicity with an interval of 7 units of molecular mass observed seems to be caused by the presence of complete homologuous series of compounds within the range of low molecular masses.", "contents": "[Notes about molecular weights of aroma compounds]. Classification of 2000 food aroma compounds as to their molecular weight shows an accumulation in the range of 135 to 155, and an upper limit of 310. Molecular masses of substances with high aroma effectiveness are below 200. As aroma compounds are composed only of the elements C, H, O, N and S, even numbers of molecular masses predominate among these substances. A periodicity with an interval of 7 units of molecular mass observed seems to be caused by the presence of complete homologuous series of compounds within the range of low molecular masses.", "PMID": 958345}
{"id": "pubmed23n0032_3061", "title": "[Bouquet qualification of wines by gas chromatography. 1. Experimental method for gas phase analysis].", "content": "In the course of our investigations we tried to find a method for the bouquet qualification of wines by gas chromatography. For this purpose, instead of aroma research studies requiring complex equipment and laboursome precise laboratory work, we developed a method for head space analysis functioning with simple experimental instruments and being sufficiently fast for serial routine work. By the method developed, the head space of a great number of samples taken from 7 different wine-types have been successfully examined. Errors of measurement were checked by standard deviation analysis and the values obtained (3,23%) were found satisfactory. According to the above, our head space analysis may provide a satisfactory basis for elaborating a method for the instrumental bouquet qualification of wines.", "contents": "[Bouquet qualification of wines by gas chromatography. 1. Experimental method for gas phase analysis]. In the course of our investigations we tried to find a method for the bouquet qualification of wines by gas chromatography. For this purpose, instead of aroma research studies requiring complex equipment and laboursome precise laboratory work, we developed a method for head space analysis functioning with simple experimental instruments and being sufficiently fast for serial routine work. By the method developed, the head space of a great number of samples taken from 7 different wine-types have been successfully examined. Errors of measurement were checked by standard deviation analysis and the values obtained (3,23%) were found satisfactory. According to the above, our head space analysis may provide a satisfactory basis for elaborating a method for the instrumental bouquet qualification of wines.", "PMID": 958346}
{"id": "pubmed23n0032_3062", "title": "[Bouquet qualification of wines by gas chromatography. 2. Development of measuring unit for bouquet qualification by determination of relationship of chromatographic peak areas and conditions of application].", "content": "In a previous paper we described a method by which routine head-space analysis of wines can be performed with sufficiently accurate results by means of gas chromatography. From the results thus obtained, with application of organoleptic data, chemical and classical analytical parameters of aromatic changes during seasoning and storing wines as well as mathematical-statistical data of the method elaborated, a mathematical relationship has been derived between gas chromatographic peak areas characteristic of different aroma components and the bouquet of wine. Reliability of the method has been verified by the fact that, in spite of the small number of samples, the bouquet of seven different types of wine could be discriminated in 95,45% probalility. The method allows arbitrary choice in the number of samples and in the degree of reliability required, in investigating the quality of a wine. For this purpose, another mathematical relationship has been set up to provide criteria for the distinction of the quality of bouquets. By this method, the bouquet of various types of wines or within an identical type, the bouquet of different vintages and/or bottlings can be determined.", "contents": "[Bouquet qualification of wines by gas chromatography. 2. Development of measuring unit for bouquet qualification by determination of relationship of chromatographic peak areas and conditions of application]. In a previous paper we described a method by which routine head-space analysis of wines can be performed with sufficiently accurate results by means of gas chromatography. From the results thus obtained, with application of organoleptic data, chemical and classical analytical parameters of aromatic changes during seasoning and storing wines as well as mathematical-statistical data of the method elaborated, a mathematical relationship has been derived between gas chromatographic peak areas characteristic of different aroma components and the bouquet of wine. Reliability of the method has been verified by the fact that, in spite of the small number of samples, the bouquet of seven different types of wine could be discriminated in 95,45% probalility. The method allows arbitrary choice in the number of samples and in the degree of reliability required, in investigating the quality of a wine. For this purpose, another mathematical relationship has been set up to provide criteria for the distinction of the quality of bouquets. By this method, the bouquet of various types of wines or within an identical type, the bouquet of different vintages and/or bottlings can be determined.", "PMID": 958347}
{"id": "pubmed23n0032_3063", "title": "Technological studies on the dehydration of the Nile bolti fish (Tilapia nilotica).", "content": "Chemical and microbiological analysis of bolti fish: moisture, chloride, fat, total volatile bases (T.V.B.), thiobarbituric acid value (T.B.A.), total bacterial count and coliforms were estimated in fresh bolti fish, after brining, drying and during storage at room temperature for 3 months. The moisture content decreased after drying but become stable during the storage period. Chloride increased after bringing but no noticeable changes were observed after drying and during 3 months storage. The fat content was not affected by previously processes. T.V.B. and T.B.A. increased after brining, drying and throughout storage period. The rate of increase was higher for dehydrated samples than for sun-dried ones. The reduction of total bacterial count is due to the high salt content and the lack of free water in fish tissues after drying. Coliforms were not present after brining, drying and throughout the storage periods.", "contents": "Technological studies on the dehydration of the Nile bolti fish (Tilapia nilotica). Chemical and microbiological analysis of bolti fish: moisture, chloride, fat, total volatile bases (T.V.B.), thiobarbituric acid value (T.B.A.), total bacterial count and coliforms were estimated in fresh bolti fish, after brining, drying and during storage at room temperature for 3 months. The moisture content decreased after drying but become stable during the storage period. Chloride increased after bringing but no noticeable changes were observed after drying and during 3 months storage. The fat content was not affected by previously processes. T.V.B. and T.B.A. increased after brining, drying and throughout storage period. The rate of increase was higher for dehydrated samples than for sun-dried ones. The reduction of total bacterial count is due to the high salt content and the lack of free water in fish tissues after drying. Coliforms were not present after brining, drying and throughout the storage periods.", "PMID": 958348}
{"id": "pubmed23n0032_3064", "title": "Lipids of two Cassia species and Datura stramonium seeds.", "content": "The fatty acid constitution of three seed-oils, namely, Cassia alexandrina, Cassia italica and Datura stramonium, were carried out using gas chromatographic analysis. The glyceride structure of these oils was computed using the technique of pancreatic lipase hydrolysis. The phospholipids fraction of these oils were identified using thin layer chromatography, also the fatty acid constitution of the phosphilipids were determined quantitatively by gas liquid chromatography.", "contents": "Lipids of two Cassia species and Datura stramonium seeds. The fatty acid constitution of three seed-oils, namely, Cassia alexandrina, Cassia italica and Datura stramonium, were carried out using gas chromatographic analysis. The glyceride structure of these oils was computed using the technique of pancreatic lipase hydrolysis. The phospholipids fraction of these oils were identified using thin layer chromatography, also the fatty acid constitution of the phosphilipids were determined quantitatively by gas liquid chromatography.", "PMID": 958349}
{"id": "pubmed23n0032_3065", "title": "[Measurement of emulsion stability].", "content": "For the routine assessment of the stability of water-in-oil emulsions, the authors suggest to determine the beginning of de-emulsification or to measure the stability constant at 38 degrees C., since these methods yield fairly reproducible results and require but little time and laboratory outfit. More accurate results will be obtained only by conductance measurements.", "contents": "[Measurement of emulsion stability]. For the routine assessment of the stability of water-in-oil emulsions, the authors suggest to determine the beginning of de-emulsification or to measure the stability constant at 38 degrees C., since these methods yield fairly reproducible results and require but little time and laboratory outfit. More accurate results will be obtained only by conductance measurements.", "PMID": 958350}
{"id": "pubmed23n0032_3066", "title": "[Behavior of various blood parameters of carbohydrate and fat metabolism of light and heavy-weight rats of varying age and different diet groups].", "content": "The sera from light and heavy-weight rats varying in age and belonging to different groups on, respectively, pellets or diets containing 3% or 50% of fat were analysed for pyruvate, lactate, aceto-acetate, beta-hydroxybutyrate, free fatty acids, triglycerides and total cholesterol. There were no significant differences in these parameters between light and heavy-weight animals of the same diet group. The total cholesterol content increased with age in the rats which were fed pellets. These findings are compared with previous results of the authors and discussed in the light of recent literature.", "contents": "[Behavior of various blood parameters of carbohydrate and fat metabolism of light and heavy-weight rats of varying age and different diet groups]. The sera from light and heavy-weight rats varying in age and belonging to different groups on, respectively, pellets or diets containing 3% or 50% of fat were analysed for pyruvate, lactate, aceto-acetate, beta-hydroxybutyrate, free fatty acids, triglycerides and total cholesterol. There were no significant differences in these parameters between light and heavy-weight animals of the same diet group. The total cholesterol content increased with age in the rats which were fed pellets. These findings are compared with previous results of the authors and discussed in the light of recent literature.", "PMID": 958351}
{"id": "pubmed23n0032_3067", "title": "[Vitamin B1 determination in ready-to-use infant food].", "content": "The authors report of a modification to the vitamin B1 determination (thiochrome method) according to the Specification TGL 18230. Extract purification is considerably simplified by using the KPS cation exchanger. The measurements were performed by means of the Spekol spectrophotometer with fluorescence attachment. In this way, also industrial laboratories with modest outfit are enable to make vitamin B1 determinations. Already after 2-3 months of storage, the thiamine values of the fruit and vegetable baby foods tested lay in part at the lower limit of the stated values. Possibilities of improving the thiamine values are discussed.", "contents": "[Vitamin B1 determination in ready-to-use infant food]. The authors report of a modification to the vitamin B1 determination (thiochrome method) according to the Specification TGL 18230. Extract purification is considerably simplified by using the KPS cation exchanger. The measurements were performed by means of the Spekol spectrophotometer with fluorescence attachment. In this way, also industrial laboratories with modest outfit are enable to make vitamin B1 determinations. Already after 2-3 months of storage, the thiamine values of the fruit and vegetable baby foods tested lay in part at the lower limit of the stated values. Possibilities of improving the thiamine values are discussed.", "PMID": 958352}
{"id": "pubmed23n0032_3068", "title": "[Germ reduction in technical enzyme preparations with special regard to gamma irradiation].", "content": "For toxicological and hygienic reasons, the use of enzymes as technical additives is not admitted in various food industries if the germ counts of the preparations to be used exceed the respective tolerance values. In this connection, the authors report some results from attempts to reduce the germ content of a protease preparation, especially by gamma radiation. A gamma ray dose of I Mrad permitted to obtain germ-free preparations without considerable loss of enzyme activity.", "contents": "[Germ reduction in technical enzyme preparations with special regard to gamma irradiation]. For toxicological and hygienic reasons, the use of enzymes as technical additives is not admitted in various food industries if the germ counts of the preparations to be used exceed the respective tolerance values. In this connection, the authors report some results from attempts to reduce the germ content of a protease preparation, especially by gamma radiation. A gamma ray dose of I Mrad permitted to obtain germ-free preparations without considerable loss of enzyme activity.", "PMID": 958353}
{"id": "pubmed23n0032_3069", "title": "[Significance of byssochlamic acid in fruit juice mold].", "content": "The authors describe a method for the determination of byssochlamic acid in fruit juice. After extraction and purification, byssochlamic acid is separated by thin-layer chromatography on HF 254 silica gel. The quenching of fluorescence of byssochlamic acid is estimated quantitatively on the plate by means of the thin-layer attachment of a fluorescence spectrophotometer using reference substances. For fruit juices the recovery rate is 80%; the limit of detection lies at 0.5 p.p.m. Byssochlamic acid could not be detected in commercially-available fruit juice and neither in juices produced of fruits which had spontaneously got mouldy.", "contents": "[Significance of byssochlamic acid in fruit juice mold]. The authors describe a method for the determination of byssochlamic acid in fruit juice. After extraction and purification, byssochlamic acid is separated by thin-layer chromatography on HF 254 silica gel. The quenching of fluorescence of byssochlamic acid is estimated quantitatively on the plate by means of the thin-layer attachment of a fluorescence spectrophotometer using reference substances. For fruit juices the recovery rate is 80%; the limit of detection lies at 0.5 p.p.m. Byssochlamic acid could not be detected in commercially-available fruit juice and neither in juices produced of fruits which had spontaneously got mouldy.", "PMID": 958354}
{"id": "pubmed23n0032_3070", "title": "Characteristics of prickly lettuce seed oil in relation to methods of extraction.", "content": "Samples of seed oil of prickly lettuce (Lactuca Sacriola oleifera) which had been obtained by pressing or by extracting with acetone, ethyl ether, petroleum ether or carbon tetrachloride were analysed for the following parameters: viscosity, saponification number, iodine number, thiocyanogen value, unsaponifiable matter, free fatty acids, peroxide number and fatty acid composition. The different parameters varied in part considerably in relation to the method of production (pressing or solvent extraction) and to the solvent. It is tried to interprete these relationships.", "contents": "Characteristics of prickly lettuce seed oil in relation to methods of extraction. Samples of seed oil of prickly lettuce (Lactuca Sacriola oleifera) which had been obtained by pressing or by extracting with acetone, ethyl ether, petroleum ether or carbon tetrachloride were analysed for the following parameters: viscosity, saponification number, iodine number, thiocyanogen value, unsaponifiable matter, free fatty acids, peroxide number and fatty acid composition. The different parameters varied in part considerably in relation to the method of production (pressing or solvent extraction) and to the solvent. It is tried to interprete these relationships.", "PMID": 958356}
{"id": "pubmed23n0032_3071", "title": "Control of postharvest decay in fruits and vegetables by irradiation.", "content": "This paper deals with the effect of postharvest gamma-irradiation of strawberries and carrots on their organoleptic quality, storage capacity and chemical composition. Radiation doses of 25 krad for strawberries and 75 krad for carrots were chosen as optimum doses not causing significant changes in the organoleptic quality. We found that strawberries and carrots irradiated at a doseof 25 and 75 krad respectively, retained its microbiological stability even during storage at 12 degrees C (strawberries) and at 25-30 degrees C (carrots), respectively. It was also found that changes in chemical composition depend on storage temperature and time and are not affected substantially by irradiation.", "contents": "Control of postharvest decay in fruits and vegetables by irradiation. This paper deals with the effect of postharvest gamma-irradiation of strawberries and carrots on their organoleptic quality, storage capacity and chemical composition. Radiation doses of 25 krad for strawberries and 75 krad for carrots were chosen as optimum doses not causing significant changes in the organoleptic quality. We found that strawberries and carrots irradiated at a doseof 25 and 75 krad respectively, retained its microbiological stability even during storage at 12 degrees C (strawberries) and at 25-30 degrees C (carrots), respectively. It was also found that changes in chemical composition depend on storage temperature and time and are not affected substantially by irradiation.", "PMID": 958357}
{"id": "pubmed23n0032_3072", "title": "Histochemical, biochemical and ultrastructural studies on the liver of fasted rats.", "content": "The subjection of rats with body weight 150 +/- 10 g to complete starvation for a period of four days leads to a diminution of total protein, total lipids, blood sugar, body weight and liver weight. Lipid dystrophy develops in the liver, as well as deposition of lipofuscin-like pigment and atrophy. Lipid dystrophy and desposition of pigment increase during the first three days and abruptly decrease during the fourth. Atrophy is a progressive process. The delineation of three phases in the atrophic - dystrophic process is possible with the application of histological, enzyme-histochemical, morphometric, biochemical and electron microscopic methods: Phase I (first 24 hours) - a common adaptive phase. It engages both the liver, which must utilize the increased nutrients from the organism depots and the homeostatic mechanisms of the organism as a whole. Phase II - (second and third 24 hours) - alterative-restorative, manifested markedly at the liver parenchimal level and especially by autophagic lysosome function. Phase III - (fourth 24 hours) - alterative. Exhaustion of adaptive-restorative liver process (and the hepatocyte in particular), and the organism as a whole as well.", "contents": "Histochemical, biochemical and ultrastructural studies on the liver of fasted rats. The subjection of rats with body weight 150 +/- 10 g to complete starvation for a period of four days leads to a diminution of total protein, total lipids, blood sugar, body weight and liver weight. Lipid dystrophy develops in the liver, as well as deposition of lipofuscin-like pigment and atrophy. Lipid dystrophy and desposition of pigment increase during the first three days and abruptly decrease during the fourth. Atrophy is a progressive process. The delineation of three phases in the atrophic - dystrophic process is possible with the application of histological, enzyme-histochemical, morphometric, biochemical and electron microscopic methods: Phase I (first 24 hours) - a common adaptive phase. It engages both the liver, which must utilize the increased nutrients from the organism depots and the homeostatic mechanisms of the organism as a whole. Phase II - (second and third 24 hours) - alterative-restorative, manifested markedly at the liver parenchimal level and especially by autophagic lysosome function. Phase III - (fourth 24 hours) - alterative. Exhaustion of adaptive-restorative liver process (and the hepatocyte in particular), and the organism as a whole as well.", "PMID": 958358}
{"id": "pubmed23n0032_3073", "title": "[Behavior of some parameters of lipid and energy metabolism. 1. Behavior of the stationary concentrations of acetyl CoA, acetoacetate and adenosine phosphates in liver, as well as oxygen consumption and P/O ratios in liver homogenates of growing rats on diets differing in fat content].", "content": "Young rats were maintained on diets differing in fat content. The determination of certain parameters of the lipid and the energy metabolism in the liver showed that a high-fat diet resulted in a reduction of the stationary level of acetyl CoA, an increase in the concentration of acetoacetate and a reduction of the adenosine triphosphate content and the so-called energy charge. In case of intact respiratory chain phosphorylation, the oxygen consumption of the respective liver homogenates was simultaneously increased. The results obtained with a high-fat diet are indicative of the attainment of a metabolic state which seems to be typical of metabolic regulations in growing rats subjected to anabolic lipometabolism.", "contents": "[Behavior of some parameters of lipid and energy metabolism. 1. Behavior of the stationary concentrations of acetyl CoA, acetoacetate and adenosine phosphates in liver, as well as oxygen consumption and P/O ratios in liver homogenates of growing rats on diets differing in fat content]. Young rats were maintained on diets differing in fat content. The determination of certain parameters of the lipid and the energy metabolism in the liver showed that a high-fat diet resulted in a reduction of the stationary level of acetyl CoA, an increase in the concentration of acetoacetate and a reduction of the adenosine triphosphate content and the so-called energy charge. In case of intact respiratory chain phosphorylation, the oxygen consumption of the respective liver homogenates was simultaneously increased. The results obtained with a high-fat diet are indicative of the attainment of a metabolic state which seems to be typical of metabolic regulations in growing rats subjected to anabolic lipometabolism.", "PMID": 958359}
{"id": "pubmed23n0032_3074", "title": "[Behavior of some parameters of lipid and energy metabolism. 2. Activity of citrate synthase, ATP citrate lyase, fatty acid synthase, and glucose-6-phosphate dehydrogenase in liver of growing rats on diets differing in fat content].", "content": "In continuation of previous investigations, the authors studied the behaviour of the activities of certain enzymes (citrate synthase, adenosine triphosphate citrate lyase, fatty acid synthase and glucose-6-phosphate dehydrogenase) in the livers of growing rats on diets differing in fat content. A high-fat diet resulted in a reduction of the activities of fatty acid synthase, glucose-6-phosphate dehydrogenase and adenosine triphosphate citrate lyase, whereas the activity of citrate synthase increased, which is interpreted in the sense of an acceleration of the introduction of acetyl residues into the citrate cycle for the purpose of oxidative final degradation, and as a removal of acetyl CoA for the purpose of fatty acid synthesis.", "contents": "[Behavior of some parameters of lipid and energy metabolism. 2. Activity of citrate synthase, ATP citrate lyase, fatty acid synthase, and glucose-6-phosphate dehydrogenase in liver of growing rats on diets differing in fat content]. In continuation of previous investigations, the authors studied the behaviour of the activities of certain enzymes (citrate synthase, adenosine triphosphate citrate lyase, fatty acid synthase and glucose-6-phosphate dehydrogenase) in the livers of growing rats on diets differing in fat content. A high-fat diet resulted in a reduction of the activities of fatty acid synthase, glucose-6-phosphate dehydrogenase and adenosine triphosphate citrate lyase, whereas the activity of citrate synthase increased, which is interpreted in the sense of an acceleration of the introduction of acetyl residues into the citrate cycle for the purpose of oxidative final degradation, and as a removal of acetyl CoA for the purpose of fatty acid synthesis.", "PMID": 958360}
{"id": "pubmed23n0032_3075", "title": "[New methods for evaluation and analysis of organoleptic qualities of foods and for calculation of changes. 9. Dependence of irradiation flavor of apple juice and concentrate on the gamma radiation dose].", "content": "Various authors have defined organoleptically the concept \"cooking flavour\" for heat-treated apple juice and concentrate. In a similar way, the \"irradiation flavour\" caused by gamma radiation is defined in the present paper. It has been found that the irradiation flavour is attributable to low-boiling substances which appear already in the first fraction if the modified MICKO distillation is performed. Tetrahydrofuran (THF) has been identified as the key substance in irradiation flavour. As further evidence may be quoted the similarity of the intensity of off-flavour which was observed also by gas chromatography at the same times of retention, and the removal of the irradiation flavour by means of a THF-specific agent (mercury acetate). In connection with THF solutions added to non-irradiated apple juice, the authors employed the technique of \"subjective olfactometry\", using the \"absolute value scale\", to establish mathematical correlations between the intensity of irradiation flavour and the concentration of off-flavour products in apple juice or concentrate. The dependence of the intensity of irradiation flavour on the THF concentration and the gamma radiation dose, respectively, obeyed an exponential function. Thus, it has been possible to calculate the amounts of THF formed by radiation doses varying from 0.64 to 2.55 Mrad. Furthermore, the authors determined the minimum radiation dose for 12% apple juice and for 67% apple concentrate at which the irradiation flavour is first perceived (treshold of perception).", "contents": "[New methods for evaluation and analysis of organoleptic qualities of foods and for calculation of changes. 9. Dependence of irradiation flavor of apple juice and concentrate on the gamma radiation dose]. Various authors have defined organoleptically the concept \"cooking flavour\" for heat-treated apple juice and concentrate. In a similar way, the \"irradiation flavour\" caused by gamma radiation is defined in the present paper. It has been found that the irradiation flavour is attributable to low-boiling substances which appear already in the first fraction if the modified MICKO distillation is performed. Tetrahydrofuran (THF) has been identified as the key substance in irradiation flavour. As further evidence may be quoted the similarity of the intensity of off-flavour which was observed also by gas chromatography at the same times of retention, and the removal of the irradiation flavour by means of a THF-specific agent (mercury acetate). In connection with THF solutions added to non-irradiated apple juice, the authors employed the technique of \"subjective olfactometry\", using the \"absolute value scale\", to establish mathematical correlations between the intensity of irradiation flavour and the concentration of off-flavour products in apple juice or concentrate. The dependence of the intensity of irradiation flavour on the THF concentration and the gamma radiation dose, respectively, obeyed an exponential function. Thus, it has been possible to calculate the amounts of THF formed by radiation doses varying from 0.64 to 2.55 Mrad. Furthermore, the authors determined the minimum radiation dose for 12% apple juice and for 67% apple concentrate at which the irradiation flavour is first perceived (treshold of perception).", "PMID": 958361}
{"id": "pubmed23n0032_3076", "title": "[Biochemical parameters of blood plasma, and body and organ weights of Wistar rats with dietarily-induced experimental obesity].", "content": "The authors investigated in rats with dietarily-induced obesity certain biochemical parameters of the blood plasma as well as body and organ weights during the dynamic and the static phase of obesity development. They determined total cholesterol, total protein, albumin, creatinine, urea nitrogen and transaminases. After 4-5 weeks, the animals on a high-diet (50% of fat) had body weights which were, on an average, by 90% higher than those of the control animals. This difference persisted during the static phase. In the animals on a high-fat diet, body length was greater. The high-fat diet (which contains a great proportion of sunflower oil) leads to a decrease of the plasma cholesterol level in obese rats. The plasma-protein bodies, creatinine and urea nitrogen values as well as those for transaminases permit, as parameters for function and damage, to draw conclusions as to kidney and liver damages in the animals on high-fat diet. There were no differences in plasma protein between the control and experimental animals. On the contrary, obese rats showed in some cases high creatinine concentrations during the dynamic phase. Differences in urea nitrogen were not observed between the two groups of animals. Increases in alanine aminotransferase were found in the animals on high-fat diet as a manifestation of fatty degeneration of the liver. A synopsis of weight curves, biochemical parameters and histological findings permits the conclusion that, besides of dietarily-induced metabolic alterations, no additional organic lesions occurred during the present animal experiment on dietarily-induced obesity.", "contents": "[Biochemical parameters of blood plasma, and body and organ weights of Wistar rats with dietarily-induced experimental obesity]. The authors investigated in rats with dietarily-induced obesity certain biochemical parameters of the blood plasma as well as body and organ weights during the dynamic and the static phase of obesity development. They determined total cholesterol, total protein, albumin, creatinine, urea nitrogen and transaminases. After 4-5 weeks, the animals on a high-diet (50% of fat) had body weights which were, on an average, by 90% higher than those of the control animals. This difference persisted during the static phase. In the animals on a high-fat diet, body length was greater. The high-fat diet (which contains a great proportion of sunflower oil) leads to a decrease of the plasma cholesterol level in obese rats. The plasma-protein bodies, creatinine and urea nitrogen values as well as those for transaminases permit, as parameters for function and damage, to draw conclusions as to kidney and liver damages in the animals on high-fat diet. There were no differences in plasma protein between the control and experimental animals. On the contrary, obese rats showed in some cases high creatinine concentrations during the dynamic phase. Differences in urea nitrogen were not observed between the two groups of animals. Increases in alanine aminotransferase were found in the animals on high-fat diet as a manifestation of fatty degeneration of the liver. A synopsis of weight curves, biochemical parameters and histological findings permits the conclusion that, besides of dietarily-induced metabolic alterations, no additional organic lesions occurred during the present animal experiment on dietarily-induced obesity.", "PMID": 958362}
{"id": "pubmed23n0032_3077", "title": "[Feasibility of using meat from swine with diet-related yellow coloration of the fat].", "content": "The utilization of the flesh of swine objected to because of dietarily-induced yellowing of the fat is an economic problem. The causes of yellowing are explained on the basis of relevant literature and results from studies of the author. The potential use of this flesh in making raw sausages is investigated. The results obtained justify such an utilization. The knowledge acquired permits to recommend this flesh for the manufacture of \"Knacker\" (a highly seasoned dried sausage) and \"Braunschweiger Mettwurst grob\" (a kind of Bologna sausage).", "contents": "[Feasibility of using meat from swine with diet-related yellow coloration of the fat]. The utilization of the flesh of swine objected to because of dietarily-induced yellowing of the fat is an economic problem. The causes of yellowing are explained on the basis of relevant literature and results from studies of the author. The potential use of this flesh in making raw sausages is investigated. The results obtained justify such an utilization. The knowledge acquired permits to recommend this flesh for the manufacture of \"Knacker\" (a highly seasoned dried sausage) and \"Braunschweiger Mettwurst grob\" (a kind of Bologna sausage).", "PMID": 958363}
{"id": "pubmed23n0032_3078", "title": "Reactions of oxidized lipids with protein. XIII. Interactions of polar groups of lipids with nonlipidic substances.", "content": "Contrary to fresh lipids, oxidized lipids form chloroform-insoluble, but methanol-soluble compounds with cellulose. The amount of chloroform-insoluble lipids is significantly higher in mixtures containing cellulose impregnated with albumin. Polar groups of oxidized lipids formed nonextractable compounds with protein more readily than polar groups of a monoglyceride. Compounds insoluble either in chloroform and in chloroform-methanol or methanol were formed in contact of oxidized lipids with protein, contrary to mixtures of lipids with sole cellulose. The formation of these nonextractable compounds is due both to the interaction of protein with hydroperoxides and with non-peroxidic oxidation products.", "contents": "Reactions of oxidized lipids with protein. XIII. Interactions of polar groups of lipids with nonlipidic substances. Contrary to fresh lipids, oxidized lipids form chloroform-insoluble, but methanol-soluble compounds with cellulose. The amount of chloroform-insoluble lipids is significantly higher in mixtures containing cellulose impregnated with albumin. Polar groups of oxidized lipids formed nonextractable compounds with protein more readily than polar groups of a monoglyceride. Compounds insoluble either in chloroform and in chloroform-methanol or methanol were formed in contact of oxidized lipids with protein, contrary to mixtures of lipids with sole cellulose. The formation of these nonextractable compounds is due both to the interaction of protein with hydroperoxides and with non-peroxidic oxidation products.", "PMID": 958364}
{"id": "pubmed23n0032_3079", "title": "[New methods for analysis and evaluation of sensory qualities of foods and calculation of changes. 10. Quantitative titrimetric determination of irradiation flavor in apple juice and concentrate and its dependence on radiation dosage].", "content": "Irradiation flavour in apple juice and concentrate which is mainly attributable, according to our previous studies, to tetrahydrofuran (THF) that may be quantitatively determined by means of the so-called mercury acetate method. This is based on the fact that the amount of a 4% solution of mercury acetate needed for removing the THF odour is proportional to the THF concentration. Using this method, the amounts of THF produced by various gamma radiation doses (varying from 0.64 to 2.55 Mrad) could be determined titrimetrically. Calculations showed that the THF concentration increases linearly with the radiation dose. Thus, the minimum radiation doses at which the irradiation flavour is first perceived (threshold of perception) may be calculated readily. The mercury acetate method is also a model case of the use of subjective olfactometry for the quantitative determination of flavour key substances in foods so far as they may be quantitatively determined by means of chemical reactions since the endpoint of titration is controlled by the sense of smell.", "contents": "[New methods for analysis and evaluation of sensory qualities of foods and calculation of changes. 10. Quantitative titrimetric determination of irradiation flavor in apple juice and concentrate and its dependence on radiation dosage]. Irradiation flavour in apple juice and concentrate which is mainly attributable, according to our previous studies, to tetrahydrofuran (THF) that may be quantitatively determined by means of the so-called mercury acetate method. This is based on the fact that the amount of a 4% solution of mercury acetate needed for removing the THF odour is proportional to the THF concentration. Using this method, the amounts of THF produced by various gamma radiation doses (varying from 0.64 to 2.55 Mrad) could be determined titrimetrically. Calculations showed that the THF concentration increases linearly with the radiation dose. Thus, the minimum radiation doses at which the irradiation flavour is first perceived (threshold of perception) may be calculated readily. The mercury acetate method is also a model case of the use of subjective olfactometry for the quantitative determination of flavour key substances in foods so far as they may be quantitatively determined by means of chemical reactions since the endpoint of titration is controlled by the sense of smell.", "PMID": 958365}
{"id": "pubmed23n0032_3080", "title": "[Nutritional study of field bean protein isolate and of spun field bean protein-casein fibers].", "content": "The amino acid composition of the different field bean protein isolates shows a good correspondence. In relation to casein the content of the whole essential amino acids is low. In the first line the low content of methionine and cystine limits the biological value of this proteins. The content of lysine is relatively high. The enzymatic in vitro-hydrolysis results in a corresponding availability of the amino acids between the field bean proteins and casein. The digestibility is very good, the biological value with less than 50 relatively low. Apart from the low content of the sulphur containing amino acids the amino acid composition of the spun field bean protein/casein(I:I) fibers corresponds with the calculated value; the enzymatic availability of the fibers is also comparable with casein. The digestibility in the nitrogen balance test is very good and the biological value of the fiber corresponds with that of casein.", "contents": "[Nutritional study of field bean protein isolate and of spun field bean protein-casein fibers]. The amino acid composition of the different field bean protein isolates shows a good correspondence. In relation to casein the content of the whole essential amino acids is low. In the first line the low content of methionine and cystine limits the biological value of this proteins. The content of lysine is relatively high. The enzymatic in vitro-hydrolysis results in a corresponding availability of the amino acids between the field bean proteins and casein. The digestibility is very good, the biological value with less than 50 relatively low. Apart from the low content of the sulphur containing amino acids the amino acid composition of the spun field bean protein/casein(I:I) fibers corresponds with the calculated value; the enzymatic availability of the fibers is also comparable with casein. The digestibility in the nitrogen balance test is very good and the biological value of the fiber corresponds with that of casein.", "PMID": 958366}
{"id": "pubmed23n0032_3081", "title": "Neutron diffraction studies of retinal rod outer segment membranes.", "content": "Neutron diffraction measurements on isolated retinal rod outer segments show that most of the visual pigment protein, rhodopsin, is embedded in the hydrophobic core of the disk membrane. A very slight outward shift of protein at the cytoplasmic side of the membrane is associated with pigment bleaching.", "contents": "Neutron diffraction studies of retinal rod outer segment membranes. Neutron diffraction measurements on isolated retinal rod outer segments show that most of the visual pigment protein, rhodopsin, is embedded in the hydrophobic core of the disk membrane. A very slight outward shift of protein at the cytoplasmic side of the membrane is associated with pigment bleaching.", "PMID": 958369}
{"id": "pubmed23n0032_3082", "title": "Model for action of local anaesthetics.", "content": "Sodium channels in nerve membranes are postulated to be surrounded by lipid molecules in the gel (or crystalline) phase. Addition of local anasethetics triggers a change in the surrounding lipids to the fluid, liquid crystalline phase, allowing the sodium channel to close with resulting local anaesthesia. The experimental evidence for this model is discussed.", "contents": "Model for action of local anaesthetics. Sodium channels in nerve membranes are postulated to be surrounded by lipid molecules in the gel (or crystalline) phase. Addition of local anasethetics triggers a change in the surrounding lipids to the fluid, liquid crystalline phase, allowing the sodium channel to close with resulting local anaesthesia. The experimental evidence for this model is discussed.", "PMID": 958412}
{"id": "pubmed23n0032_3083", "title": "Cellular basis for the T wave of the electrocardiogram.", "content": "Differences in action potential duration in different regions of the mammalian ventricle are not systematically present when quiescent tissue is first stimulated, but develop rapidly during repetitive activity. The effects of ouabain and temperature suggest the involvement of the Na+-K+ exchange pump.", "contents": "Cellular basis for the T wave of the electrocardiogram. Differences in action potential duration in different regions of the mammalian ventricle are not systematically present when quiescent tissue is first stimulated, but develop rapidly during repetitive activity. The effects of ouabain and temperature suggest the involvement of the Na+-K+ exchange pump.", "PMID": 958437}
{"id": "pubmed23n0032_3084", "title": "Lambda repressor regulates the switch between PR and Prm promoters.", "content": "The DNA region containing the Or operator, Pr and Prm promoters and their transcription initiations is sequenced. By binding to Or, repressor turns off Pr, turns on Prm and at higher concentrations turns off Prm, regulating its own synthesis. Prm mRNA is unique in beginning immediately with the initiation of translation, without a preceding leader sequence.", "contents": "Lambda repressor regulates the switch between PR and Prm promoters. The DNA region containing the Or operator, Pr and Prm promoters and their transcription initiations is sequenced. By binding to Or, repressor turns off Pr, turns on Prm and at higher concentrations turns off Prm, regulating its own synthesis. Prm mRNA is unique in beginning immediately with the initiation of translation, without a preceding leader sequence.", "PMID": 958438}
{"id": "pubmed23n0032_3085", "title": "Significance of impulse activity in the transformation of skeletal muscle type.", "content": "The changes which follow cross reinnervation of mammalian fast and slow twitch muscles may reflect a capacity of skeletal muscle to respond adaptively to different functional requirements. This interpretation is supported by experiments in which long-term electrical stimulation was used both to reproduce and to oppose the effects of cross reinnervation.", "contents": "Significance of impulse activity in the transformation of skeletal muscle type. The changes which follow cross reinnervation of mammalian fast and slow twitch muscles may reflect a capacity of skeletal muscle to respond adaptively to different functional requirements. This interpretation is supported by experiments in which long-term electrical stimulation was used both to reproduce and to oppose the effects of cross reinnervation.", "PMID": 958462}
{"id": "pubmed23n0032_3086", "title": "Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight.", "content": "Adipokinetic hormone, isolated from locust corpora cardiaca, has been identified as a blocked peptide: PCA-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2. The detailed structure is based on mass spectrometric data, substantiated in part by dansyl-Edman and carboxypeptidase data on thermolytic fragments. This is the first peptide hormone from an insect neuroendocrine organ to be fully characterised.", "contents": "Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight. Adipokinetic hormone, isolated from locust corpora cardiaca, has been identified as a blocked peptide: PCA-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-NH2. The detailed structure is based on mass spectrometric data, substantiated in part by dansyl-Edman and carboxypeptidase data on thermolytic fragments. This is the first peptide hormone from an insect neuroendocrine organ to be fully characterised.", "PMID": 958472}
{"id": "pubmed23n0032_3087", "title": "Complementary base pairing and the origin of substitution mutations.", "content": "On the basis of chemical considerations and model building, the Watson-Crick concept of complementary base pairing is extended to a wider range of DNA pairs that A-T and G-C (including A-C, G-T, A-A, G-G and G-A) by invoking imino or enol tautomers (or protonated species) and synisomers. The virtual absence of these additional base pairs from DNA is explained in terms of the low frequency with which these unfavoured forms occur and the two-step mechanism of DNA synthesis, whereby residues are first incorporated by the DNA polymerase and then checked. This base-pairing hypothesis is used to explain the origin, nature and level of spontaneous substitution mutations, their enhancement by base analogues, and the unique effects of certain mutator alleles.", "contents": "Complementary base pairing and the origin of substitution mutations. On the basis of chemical considerations and model building, the Watson-Crick concept of complementary base pairing is extended to a wider range of DNA pairs that A-T and G-C (including A-C, G-T, A-A, G-G and G-A) by invoking imino or enol tautomers (or protonated species) and synisomers. The virtual absence of these additional base pairs from DNA is explained in terms of the low frequency with which these unfavoured forms occur and the two-step mechanism of DNA synthesis, whereby residues are first incorporated by the DNA polymerase and then checked. This base-pairing hypothesis is used to explain the origin, nature and level of spontaneous substitution mutations, their enhancement by base analogues, and the unique effects of certain mutator alleles.", "PMID": 958482}
{"id": "pubmed23n0032_3088", "title": "Base pairing and fidelity in codon-anticodon interaction.", "content": "Base pairing in codon-anticodon interaction has been investigated in order to understand the basis on which particular base pairs have been selected for or against participation at the wobble position and the basis for codon-anticodon infidelity.", "contents": "Base pairing and fidelity in codon-anticodon interaction. Base pairing in codon-anticodon interaction has been investigated in order to understand the basis on which particular base pairs have been selected for or against participation at the wobble position and the basis for codon-anticodon infidelity.", "PMID": 958483}
{"id": "pubmed23n0032_3089", "title": "Crystal structure of elastase-substrate complex at -- 55 degrees C.", "content": "The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.", "contents": "Crystal structure of elastase-substrate complex at -- 55 degrees C. The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.", "PMID": 958484}
{"id": "pubmed23n0032_3090", "title": "The effect of inhibitors of extraneuronal uptake on the distribution of 3H-(+/-)noradrenaline in nerve-free rabbit aortic strips.", "content": "1. Nerve-free rabbit aortic strips were exposed to 1.18muM 3H-(+/-)noradrenaline for 30 min. When either MAO or COMT was inhibited, far more O-methylated (MAO inhibited) than deaminated metabolites (COMT inhibited) were formed during the incubation. The accumulation of unchanged amine in the extraneuronal stores was inversely related to the rate of metabolism. 2. After inhibition of both metabolizing enzymes, nerve-free strips were first incubated with the amine and then washed out with amine free solution. Compartmental analysis of the efflux curves showed that two extraneuronal compartments were involved in accumulation (with half times of efflux of 3 and 11 min, respectively). 3. 86muM corticosterone or 30muM phenoxybenzamine greatly decreased the accumulation of noradrenaline in these two compartments. 4. When corticosterone or phenoxybenzamine was added to the wash out solution only, the half time of the efflux from both compartments was greatly increased. However, this effect was seen only after inhibition of COMT and not after inhibition of MAO only (the filling of the extraneuronal stores with unchanged noradrenaline being better after inhibition of COMT than when this enzyme was intact). The effect of corticosterone appeared to be reversible, that of phenoxybenzamine irreversible. 5. Analysis of the efflux of metabolites (in experiments in which only one enzyme was inhibited) indicated that corticosterone affected the efflux of noradrenaline but not that of the metabolites. 6. When either COMT or MAO was inhibited throughout the experiment, very little or no metabolism of noradrenaline occured during prolonged wash out. On the other hand, dis-inhibition of COMT during wash out (by the omission of U-0521 from the wash out solution after it had been present during the initial incubation) revealed that noradrenaline, stored extraneuronally during the initial incubation, is quickly O-methylated during wash out, especially when the efflux of the parent amine is inhibited by corticosterone. 7. The results show that COMT is the major extraneuronal noradrenaline-metabolizing enzyme of rabbit aorta, that inhibition of COMT is a pre-requisite for any corticosterone-sensitive accumulation of noradrenaline, that there are two important extraneuronal compartments (compartments III and IV; Henseling et al., 1976a), and that inhibitors of extraneuronal uptake inhibit both, influx and efflux of noradrenaline.", "contents": "The effect of inhibitors of extraneuronal uptake on the distribution of 3H-(+/-)noradrenaline in nerve-free rabbit aortic strips. 1. Nerve-free rabbit aortic strips were exposed to 1.18muM 3H-(+/-)noradrenaline for 30 min. When either MAO or COMT was inhibited, far more O-methylated (MAO inhibited) than deaminated metabolites (COMT inhibited) were formed during the incubation. The accumulation of unchanged amine in the extraneuronal stores was inversely related to the rate of metabolism. 2. After inhibition of both metabolizing enzymes, nerve-free strips were first incubated with the amine and then washed out with amine free solution. Compartmental analysis of the efflux curves showed that two extraneuronal compartments were involved in accumulation (with half times of efflux of 3 and 11 min, respectively). 3. 86muM corticosterone or 30muM phenoxybenzamine greatly decreased the accumulation of noradrenaline in these two compartments. 4. When corticosterone or phenoxybenzamine was added to the wash out solution only, the half time of the efflux from both compartments was greatly increased. However, this effect was seen only after inhibition of COMT and not after inhibition of MAO only (the filling of the extraneuronal stores with unchanged noradrenaline being better after inhibition of COMT than when this enzyme was intact). The effect of corticosterone appeared to be reversible, that of phenoxybenzamine irreversible. 5. Analysis of the efflux of metabolites (in experiments in which only one enzyme was inhibited) indicated that corticosterone affected the efflux of noradrenaline but not that of the metabolites. 6. When either COMT or MAO was inhibited throughout the experiment, very little or no metabolism of noradrenaline occured during prolonged wash out. On the other hand, dis-inhibition of COMT during wash out (by the omission of U-0521 from the wash out solution after it had been present during the initial incubation) revealed that noradrenaline, stored extraneuronally during the initial incubation, is quickly O-methylated during wash out, especially when the efflux of the parent amine is inhibited by corticosterone. 7. The results show that COMT is the major extraneuronal noradrenaline-metabolizing enzyme of rabbit aorta, that inhibition of COMT is a pre-requisite for any corticosterone-sensitive accumulation of noradrenaline, that there are two important extraneuronal compartments (compartments III and IV; Henseling et al., 1976a), and that inhibitors of extraneuronal uptake inhibit both, influx and efflux of noradrenaline.", "PMID": 958502}
{"id": "pubmed23n0032_3091", "title": "Lack of epinine formation in adrenal medulla and brain of rats during cold exposure and inhibition of dopamine beta-hydroxylase.", "content": "Cold exposure of rats for 4 h and simultaneous inhibition of dopamine beta-hydroxylase by FLA-63 (25mg/kg) led to a reduction of the catecholamine content of the adrenal medulla by 46% and of the brain by 68%. Additional injections of 5 mg/kg FLA-63 4 and 9 h after beginning of the experiments, respectively, kept the catecholamine content on this low level (brain) or decreased it further (adrenal medulla). Administration of 5 mg/kg (-)DOPA together with the mono-amine oxidase inhibitor pargyline (50 mg/kg) 24 h after the first injection of FLA-63 stimulated the resynthesis. It amounted for the adrenal medulla to 20 mug/kg body weight/8 h and for the brain to 45ng/g tissue wet weight/8 h. Paper chromatographic analyses of the extracts of adrenal medulla and brain, respectively, performed at each time of the different injections, clearly identified adrenaline, noradrenaline and dopamine (in traces) in the adrenal medulla as well as noradrenaline and dopamine in the brain; epinine on the contray could not be demonstrated, not even in traces. Since at least 25 ng of epinine can be detected with certainty by our method, it can be concluded that epinine is not formed in amounts greater than 75 ng/pair adrenal glands or 37.5 ng/brain. The present results support the view that the main pathway of adrenaline biosynthesis in the suprarenal medulla and the brain proceeds via noradrenaline and not via epinine.", "contents": "Lack of epinine formation in adrenal medulla and brain of rats during cold exposure and inhibition of dopamine beta-hydroxylase. Cold exposure of rats for 4 h and simultaneous inhibition of dopamine beta-hydroxylase by FLA-63 (25mg/kg) led to a reduction of the catecholamine content of the adrenal medulla by 46% and of the brain by 68%. Additional injections of 5 mg/kg FLA-63 4 and 9 h after beginning of the experiments, respectively, kept the catecholamine content on this low level (brain) or decreased it further (adrenal medulla). Administration of 5 mg/kg (-)DOPA together with the mono-amine oxidase inhibitor pargyline (50 mg/kg) 24 h after the first injection of FLA-63 stimulated the resynthesis. It amounted for the adrenal medulla to 20 mug/kg body weight/8 h and for the brain to 45ng/g tissue wet weight/8 h. Paper chromatographic analyses of the extracts of adrenal medulla and brain, respectively, performed at each time of the different injections, clearly identified adrenaline, noradrenaline and dopamine (in traces) in the adrenal medulla as well as noradrenaline and dopamine in the brain; epinine on the contray could not be demonstrated, not even in traces. Since at least 25 ng of epinine can be detected with certainty by our method, it can be concluded that epinine is not formed in amounts greater than 75 ng/pair adrenal glands or 37.5 ng/brain. The present results support the view that the main pathway of adrenaline biosynthesis in the suprarenal medulla and the brain proceeds via noradrenaline and not via epinine.", "PMID": 958503}
{"id": "pubmed23n0032_3092", "title": "The molecular weights of plasma and intestinal kallikreins in rats.", "content": "1. The molecular weights of kallikreins of rat intestine and rat plasma have been estimated using gel filtration. 2. Extracts of pooled tissue from rat jejunum, ileum, caecum and colon activated by autolytic processes gave a single peak of kallikrein activity with a molecular weights of 33000. 3. Acid-activated rat plasma gave two peaks of kallikrein activity with molecular weight of 125000 and 61500. 4. Rat intestinal tissue contains a kinin forming enzyme having a molecular weight similar to those of glandular kallikreins and different from those of the rat plasma kallikreins.", "contents": "The molecular weights of plasma and intestinal kallikreins in rats. 1. The molecular weights of kallikreins of rat intestine and rat plasma have been estimated using gel filtration. 2. Extracts of pooled tissue from rat jejunum, ileum, caecum and colon activated by autolytic processes gave a single peak of kallikrein activity with a molecular weights of 33000. 3. Acid-activated rat plasma gave two peaks of kallikrein activity with molecular weight of 125000 and 61500. 4. Rat intestinal tissue contains a kinin forming enzyme having a molecular weight similar to those of glandular kallikreins and different from those of the rat plasma kallikreins.", "PMID": 958504}
{"id": "pubmed23n0032_3093", "title": "Selective noradrenergic denervation of the heart following intravenous injections of vinblastine or vincristine.", "content": "1. The progressive effects of a single injection (3mg/kg i.v.) of either vinblastine or vincristine on the innervation of the rat atria have been examined. 2. Functional studies were performed by stimulating electrically the noradrenergic or cholinergic nerve fibres within isolated left atria from treated and control rats. In addition, the effects of the drugs on the ultrastructure of both types of nerve fibre were examined. The noradrenergic innervation was further examined by fluorescence histochemistry and measurement of the noradrenaline content of the atria. 3. On the basis of these studies it appeared that both drugs caused degeneration of noradrenergic nerves; cholinergic nerves seemed to be unaffected. Marked effects were seen 48 h after vinblastine treatment or 30 h after vincristine treatment. 4. Evidence is presented and discussed as to whether or not this action of the vinca alkaloids can be ascribed to their well-established effects on microtubules and intra-axonal transport processes.", "contents": "Selective noradrenergic denervation of the heart following intravenous injections of vinblastine or vincristine. 1. The progressive effects of a single injection (3mg/kg i.v.) of either vinblastine or vincristine on the innervation of the rat atria have been examined. 2. Functional studies were performed by stimulating electrically the noradrenergic or cholinergic nerve fibres within isolated left atria from treated and control rats. In addition, the effects of the drugs on the ultrastructure of both types of nerve fibre were examined. The noradrenergic innervation was further examined by fluorescence histochemistry and measurement of the noradrenaline content of the atria. 3. On the basis of these studies it appeared that both drugs caused degeneration of noradrenergic nerves; cholinergic nerves seemed to be unaffected. Marked effects were seen 48 h after vinblastine treatment or 30 h after vincristine treatment. 4. Evidence is presented and discussed as to whether or not this action of the vinca alkaloids can be ascribed to their well-established effects on microtubules and intra-axonal transport processes.", "PMID": 958505}
{"id": "pubmed23n0032_3094", "title": "Pharmacokinetics of phenprocoumon in man investigated using a gas chromatographic method of drug analysis.", "content": "A gas chromatographic method for the determination of phenprocoumon (Marcumar) in serum and urine is described, which facilitates accurate values down to 0.5 mug phenprocoumon/ml serum. After the i.v. administration of 20 mg phenprocoumon in a single  dose to 4 healthy volunteers the following pharmacokinetic data were obtained: After the initial fast decrease (phase 1) of the serum level of phenprocoumon, probably due to the distribution into the different compartments is followed by a subsequent slower fall (phase 2) which occurs with a serum half-life of 157 h. The apparent distribution volume was 6.51. Analysis of the urine demonstrated that 90% of the excreted phenprocoumon detected was in the glucuronide form.", "contents": "Pharmacokinetics of phenprocoumon in man investigated using a gas chromatographic method of drug analysis. A gas chromatographic method for the determination of phenprocoumon (Marcumar) in serum and urine is described, which facilitates accurate values down to 0.5 mug phenprocoumon/ml serum. After the i.v. administration of 20 mg phenprocoumon in a single  dose to 4 healthy volunteers the following pharmacokinetic data were obtained: After the initial fast decrease (phase 1) of the serum level of phenprocoumon, probably due to the distribution into the different compartments is followed by a subsequent slower fall (phase 2) which occurs with a serum half-life of 157 h. The apparent distribution volume was 6.51. Analysis of the urine demonstrated that 90% of the excreted phenprocoumon detected was in the glucuronide form.", "PMID": 958506}
{"id": "pubmed23n0032_3095", "title": "Persistent enhancement of potassium-induced responses of the rat vas deferens by desipramine.", "content": "The effect of desipramine on the cumulative dose-response curves of noradrenaline and potassium (K+) was examined on the isolated rat vas deferens. An exposure of 10 min to 10(-7) M desipramine caused a leftward shift and an increase in the maximum response of cumulative dose-response curves of noradrenaline. Desipramine (10(-7) M), in contact with the tissue for 10 min, enhanced responses to cumulative additions of K+ without causing a consistent change in threshold concentrations. Wash-out of desipramine resulted in a rapid loss of enhanced maximum response to noradrenaline while the maximum response to K+ did not show any decrease for up to 120 min after wash-out of drug. One possible explanation for the persistent enhancement of K+-induced responses may be that desipramine causes postjuntional changes which selectively influence contractile responses of this tissue to K+.", "contents": "Persistent enhancement of potassium-induced responses of the rat vas deferens by desipramine. The effect of desipramine on the cumulative dose-response curves of noradrenaline and potassium (K+) was examined on the isolated rat vas deferens. An exposure of 10 min to 10(-7) M desipramine caused a leftward shift and an increase in the maximum response of cumulative dose-response curves of noradrenaline. Desipramine (10(-7) M), in contact with the tissue for 10 min, enhanced responses to cumulative additions of K+ without causing a consistent change in threshold concentrations. Wash-out of desipramine resulted in a rapid loss of enhanced maximum response to noradrenaline while the maximum response to K+ did not show any decrease for up to 120 min after wash-out of drug. One possible explanation for the persistent enhancement of K+-induced responses may be that desipramine causes postjuntional changes which selectively influence contractile responses of this tissue to K+.", "PMID": 958507}
{"id": "pubmed23n0032_3096", "title": "Effect of dantrolene sodium upon the activity of the hind leg muscle of the cat with local tetanus.", "content": "Cats with local tetanus were i.v. injected with dantrolene sodium. There is no effect of dantrolene sodium on the electromyogram in response to indirect stimulation of the non-poisoned muscle nor on the increased spontaneous activity of the poisoned muscle. The tension of the muscle, however, was strongly reduced.", "contents": "Effect of dantrolene sodium upon the activity of the hind leg muscle of the cat with local tetanus. Cats with local tetanus were i.v. injected with dantrolene sodium. There is no effect of dantrolene sodium on the electromyogram in response to indirect stimulation of the non-poisoned muscle nor on the increased spontaneous activity of the poisoned muscle. The tension of the muscle, however, was strongly reduced.", "PMID": 958508}
{"id": "pubmed23n0032_3097", "title": "Differential effect of raised Mg2+ concentration of K+ ion movement and on swelling in the isolated perfused rat liver poisoned by phalloidin.", "content": "The K+ release from the isolated perfused rat liver induced by phalloidin was strongly inhibited on raising the Mg2+ concentration of the perfusion medium from 0.5-40mM while, in contrast, the phalloidin induced swelling of the organ and the vasuolisation of the liver tissue was not affected.", "contents": "Differential effect of raised Mg2+ concentration of K+ ion movement and on swelling in the isolated perfused rat liver poisoned by phalloidin. The K+ release from the isolated perfused rat liver induced by phalloidin was strongly inhibited on raising the Mg2+ concentration of the perfusion medium from 0.5-40mM while, in contrast, the phalloidin induced swelling of the organ and the vasuolisation of the liver tissue was not affected.", "PMID": 958509}
{"id": "pubmed23n0032_3098", "title": "Chronopharmacokinetics of ethanol. II. Circadian rhythm in rate of blood level decline in a single subject.", "content": "A male human subject administered single, oral doses of ethanol was examined repeatedly for venous ethanol levels. Four separate trials, begun at 03.00, 09.00, 15.00, and 21.00 h, on different days yielded four different estimates of the slope of the apparently linear ethanol disappearance curve. The slopes appeared to exhibit circadian rhythmicity. In a second study of the same subject, the slope was estimated 7 times over a period of 26 h following repeated oral doses. These slopes also appeared to vary in a daily fashion. These preliminary results suggest that pharmacokinetic parameters may not be invariable with time of day.", "contents": "Chronopharmacokinetics of ethanol. II. Circadian rhythm in rate of blood level decline in a single subject. A male human subject administered single, oral doses of ethanol was examined repeatedly for venous ethanol levels. Four separate trials, begun at 03.00, 09.00, 15.00, and 21.00 h, on different days yielded four different estimates of the slope of the apparently linear ethanol disappearance curve. The slopes appeared to exhibit circadian rhythmicity. In a second study of the same subject, the slope was estimated 7 times over a period of 26 h following repeated oral doses. These slopes also appeared to vary in a daily fashion. These preliminary results suggest that pharmacokinetic parameters may not be invariable with time of day.", "PMID": 958510}
{"id": "pubmed23n0032_3099", "title": "3-Methoxytyramine as an indicator of impulse-induced dopamine release in rat brain in vivo.", "content": "3-Methoxtyramine and dopamine accumulated in vivo in rat brain after monoamine oxidase inhibition with pargyline HCl. A dose of 100 mg/kg i.p. appeared to inhibit monoamine oxidase completely and led to a linear accumulation of 3-methoxytyramine for the first 90 min. Axotomy of the ascending dopaminergic fiber tract by means of a complete transverse cerebral hemisection almost completely blocked 3-methoxytyramine formation provided that catecholamine synthesis was inhibited with H 44/68 (Methylester of alpha-methyl-p-tyrosine). The dopamine receptor agonist apomorphine, 0.5 mg/kg i.p., decreased, while the dopamine receptor antagonist haloperidol, 2 mg/kg i.p., accelerated 3-methoxytyramine formation. gamma-Butyrolactone, 750 mg/kg i.p., not only decreased 3-methoxytyramine formation per se but also effectively antagonized the haloperidol-induced increase in 3-methoxytyramine accumulation. 3-Methoxytyramine formation after inhibition of monoamine oxidase appears to be a reliable indicator of impulse-induced dopamine release and metabolism.", "contents": "3-Methoxytyramine as an indicator of impulse-induced dopamine release in rat brain in vivo. 3-Methoxtyramine and dopamine accumulated in vivo in rat brain after monoamine oxidase inhibition with pargyline HCl. A dose of 100 mg/kg i.p. appeared to inhibit monoamine oxidase completely and led to a linear accumulation of 3-methoxytyramine for the first 90 min. Axotomy of the ascending dopaminergic fiber tract by means of a complete transverse cerebral hemisection almost completely blocked 3-methoxytyramine formation provided that catecholamine synthesis was inhibited with H 44/68 (Methylester of alpha-methyl-p-tyrosine). The dopamine receptor agonist apomorphine, 0.5 mg/kg i.p., decreased, while the dopamine receptor antagonist haloperidol, 2 mg/kg i.p., accelerated 3-methoxytyramine formation. gamma-Butyrolactone, 750 mg/kg i.p., not only decreased 3-methoxytyramine formation per se but also effectively antagonized the haloperidol-induced increase in 3-methoxytyramine accumulation. 3-Methoxytyramine formation after inhibition of monoamine oxidase appears to be a reliable indicator of impulse-induced dopamine release and metabolism.", "PMID": 958511}
{"id": "pubmed23n0032_3100", "title": "The effects of methacholine and calcium deprivation on the release of the false transmitter, alpha-methyladrenaline, from the isolated rabbit heart.", "content": "1. Anaesthetized rabbits were infused for 20 min with 85 mug-kg-1-min-1(+/-)-alpha-methyladrenaline. The hearts dissected 15 min after the infusion contained 1.49 mug/g alpha-methyladrenaline; the endogenous noradrenaline content was correspondingly decreased. 2. Hearts from alpha-methyladrenaline-infused animals were isolated with the right sympathetic nerves intact and perfused. Ventricular rate, right atrial and right ventricular tensions were recorded using the transverse method. 3. Electrical stimulation (10 Hz, 1 ms, 1 min) of sympathetic nerves, perfusion with the nicotinic drug, p-aminophenethyltrimethylammonium (PAPETA) or perfusion with 54 mM KCl (high K+) solution evoked an output of both alpha-methyladrenaline and noradrenaline. The ratio of the amines in the perfusates was similar to that found in the hearts after termination of the experiments or in non-perfused hearts. 4. Methacholine perfused before and during sympathetic nerve stimulation, PAPETA or high K+ inhibited the release of both false transmitter and noradrenaline. These effects were reversed by atropine. Similarly, lowering the calcium chloride concentration of the medium from 1.8 to 0.1 mM decreased amine outputs. This was reversed by washing. 5. Tyramine evoked a preferential release of the false transmitter that was not altered by methacholine or calcium deprivation. 6. These experiments whow that the muscarinic inhibition of neuronal noradrenaline release and the requirement of calcium ions for its liberation by depolarizing stimuli can be extended to a false transmitter amine. It is suggested that the proportion of alpha-methyladrenaline to noradrenaline occurring in the perfusate during administration of tyramine reflects the relative concentrations of the amines in the axoplasm.", "contents": "The effects of methacholine and calcium deprivation on the release of the false transmitter, alpha-methyladrenaline, from the isolated rabbit heart. 1. Anaesthetized rabbits were infused for 20 min with 85 mug-kg-1-min-1(+/-)-alpha-methyladrenaline. The hearts dissected 15 min after the infusion contained 1.49 mug/g alpha-methyladrenaline; the endogenous noradrenaline content was correspondingly decreased. 2. Hearts from alpha-methyladrenaline-infused animals were isolated with the right sympathetic nerves intact and perfused. Ventricular rate, right atrial and right ventricular tensions were recorded using the transverse method. 3. Electrical stimulation (10 Hz, 1 ms, 1 min) of sympathetic nerves, perfusion with the nicotinic drug, p-aminophenethyltrimethylammonium (PAPETA) or perfusion with 54 mM KCl (high K+) solution evoked an output of both alpha-methyladrenaline and noradrenaline. The ratio of the amines in the perfusates was similar to that found in the hearts after termination of the experiments or in non-perfused hearts. 4. Methacholine perfused before and during sympathetic nerve stimulation, PAPETA or high K+ inhibited the release of both false transmitter and noradrenaline. These effects were reversed by atropine. Similarly, lowering the calcium chloride concentration of the medium from 1.8 to 0.1 mM decreased amine outputs. This was reversed by washing. 5. Tyramine evoked a preferential release of the false transmitter that was not altered by methacholine or calcium deprivation. 6. These experiments whow that the muscarinic inhibition of neuronal noradrenaline release and the requirement of calcium ions for its liberation by depolarizing stimuli can be extended to a false transmitter amine. It is suggested that the proportion of alpha-methyladrenaline to noradrenaline occurring in the perfusate during administration of tyramine reflects the relative concentrations of the amines in the axoplasm.", "PMID": 958512}
{"id": "pubmed23n0032_3101", "title": "Factors determining the rate of relaxation of rabbit aortic strips after an exposure to noradrenaline.", "content": "1. When noradrenaline distributes exclusively into the: extraneuronal o-methylation of about one third of the extracellular space (i.e., when both, neuronal and extraneuronal uptake mechanisms are blocked by the presence of cocaine and corticosterone), the response of rabbit aortic strips to noradrenaline is concentration-dependent and relaxation curves (obtained during wash out and starting from different heights) are parallel. Under these conditions the \"time required for relaxation to 50 or 20% of the initial contraction\" (t50 and t20, respectively) is positively correlated with the initial height of contraction. 2. Measurements of the rate of relaxation that are independent of the height of the initial contraction are obtained either by appropriate correction of the t50 (or t20) or by determination of the \"average rate of relaxation down to the 25% level of maximum contraction\". 3. Relaxation experiments should a) be carried out with initial contractions of less than 75% of maximum and b) use a measure of the rate of relaxation that is independent of the initial height of contraction. 4. When the experimental conditions favour the extraneuronal accumulation of noradrenaline, corticosterone affects the relaxation of the strips in a way which is consistent with the view that efflux of unchanged amine from extraneuronal stores influences the rate of relaxation. 5. When extraneuonal uptake is operative and accumulation of noradrenaline is poor (i.e., when extraneuronal catechol-O-methyl transferase is intact), corticosterone affects relaxation in a way which is consistent with the view that normally the extraneuronal system serves as a site of loss. Under these conditions corticosterone prevented, during the first 5 min of wash out, the extracellularly distributed amine. 6. It is concluded that the experimental conditions of relaxation experiments determine whether the noradrenaline stores serve as a source of efflux of unchanged amine or as a site of loss.", "contents": "Factors determining the rate of relaxation of rabbit aortic strips after an exposure to noradrenaline. 1. When noradrenaline distributes exclusively into the: extraneuronal o-methylation of about one third of the extracellular space (i.e., when both, neuronal and extraneuronal uptake mechanisms are blocked by the presence of cocaine and corticosterone), the response of rabbit aortic strips to noradrenaline is concentration-dependent and relaxation curves (obtained during wash out and starting from different heights) are parallel. Under these conditions the \"time required for relaxation to 50 or 20% of the initial contraction\" (t50 and t20, respectively) is positively correlated with the initial height of contraction. 2. Measurements of the rate of relaxation that are independent of the height of the initial contraction are obtained either by appropriate correction of the t50 (or t20) or by determination of the \"average rate of relaxation down to the 25% level of maximum contraction\". 3. Relaxation experiments should a) be carried out with initial contractions of less than 75% of maximum and b) use a measure of the rate of relaxation that is independent of the initial height of contraction. 4. When the experimental conditions favour the extraneuronal accumulation of noradrenaline, corticosterone affects the relaxation of the strips in a way which is consistent with the view that efflux of unchanged amine from extraneuronal stores influences the rate of relaxation. 5. When extraneuonal uptake is operative and accumulation of noradrenaline is poor (i.e., when extraneuronal catechol-O-methyl transferase is intact), corticosterone affects relaxation in a way which is consistent with the view that normally the extraneuronal system serves as a site of loss. Under these conditions corticosterone prevented, during the first 5 min of wash out, the extracellularly distributed amine. 6. It is concluded that the experimental conditions of relaxation experiments determine whether the noradrenaline stores serve as a source of efflux of unchanged amine or as a site of loss.", "PMID": 958513}
{"id": "pubmed23n0032_3102", "title": "Factors responsible for reduced pharmacological activity in rats of pentetrazol administered orally.", "content": "It was the aim of this investigation to find out why pentetrazol (PTZ) administered orally to rats has considerably lower convulsive effectiveness than PTZ injected parenterally. It could be demonstrated that PTZ is distributed to and eliminated from all organs analyzed, without any sign of storage or of redistribution. Biological halftime was found to be about 3.5 h after subcutaneous injection. PTZ is absorbed slowly from the gastrointestinal tract because PTZ given orally is retained in the stomach for many hours. PTZ absorption is slow from the stomach while it is rapid from the small intestine. Food taken in before and after PTZ administration decreases the rate of PTZ absorption. Retention in the stomach of PTZ given orally is probably due to delayed gastric emptying since PTZ is able to reduce the basal tone and the acetylcholine-induced contractions of isolated preparations of the fundus of the stomach.", "contents": "Factors responsible for reduced pharmacological activity in rats of pentetrazol administered orally. It was the aim of this investigation to find out why pentetrazol (PTZ) administered orally to rats has considerably lower convulsive effectiveness than PTZ injected parenterally. It could be demonstrated that PTZ is distributed to and eliminated from all organs analyzed, without any sign of storage or of redistribution. Biological halftime was found to be about 3.5 h after subcutaneous injection. PTZ is absorbed slowly from the gastrointestinal tract because PTZ given orally is retained in the stomach for many hours. PTZ absorption is slow from the stomach while it is rapid from the small intestine. Food taken in before and after PTZ administration decreases the rate of PTZ absorption. Retention in the stomach of PTZ given orally is probably due to delayed gastric emptying since PTZ is able to reduce the basal tone and the acetylcholine-induced contractions of isolated preparations of the fundus of the stomach.", "PMID": 958514}
{"id": "pubmed23n0032_3103", "title": "[Dynamic characteristics of auditory analyzer neurons and their relation to selective-combination mechanisms of cerebral functional organization].", "content": "Neuron responses in the chicken torus and caudal neostriatum to signals with different ecological significance (tones \"white\" noise, species-specific signals) were studied. It is shown that the frequency band percepted by the acoustic analyzer neurons corresponds to the frequency composition of signals emanated by chickens. Neurons selectively responding to the species-specific signals (song, alarm cries) were found in the forebrain. The specificity of the functional organization of different levels of the acoustic analyzer is considered in the light of the selectively combination principle of the signals integration, which may determine the mechanism of both innate and learned memory.", "contents": "[Dynamic characteristics of auditory analyzer neurons and their relation to selective-combination mechanisms of cerebral functional organization]. Neuron responses in the chicken torus and caudal neostriatum to signals with different ecological significance (tones \"white\" noise, species-specific signals) were studied. It is shown that the frequency band percepted by the acoustic analyzer neurons corresponds to the frequency composition of signals emanated by chickens. Neurons selectively responding to the species-specific signals (song, alarm cries) were found in the forebrain. The specificity of the functional organization of different levels of the acoustic analyzer is considered in the light of the selectively combination principle of the signals integration, which may determine the mechanism of both innate and learned memory.", "PMID": 958522}
{"id": "pubmed23n0032_3104", "title": "[Responses of neurons of the first and second somatosensory zones of the cortex to peripheral, thalamic and cortical stimulation].", "content": "Experiments were performed on cats immobilized with d-tubocurarine or myorelaxin. Neuronal responses were studied in the first somatosensory cortex (SI) to the second somatosensory cortex (SII), ventroposterior nucleus (VP) and contralateral forepaw stimulation. Besides, neuronal responses in SII to SI, VP and contralateral forepaw stimulation were also studied. It was shown that in SII the percentage of neurons excited by afferent volley with two or more synaptic change-overs in the cerebral cortex was larger than in SI. Neurons of SI and SII responded to cortical stimulation ortho- and antidromically, thus confirming the existence of bilateral cortico-cortical connections. Both in SI and SII, PSPs to cortical stimulation were similar in character to PSPs in the same neurons to VP stimulation. In 50.0% of SI neurons and 37.1 of SII neurons the difference in latencies of orthodromic spike potentials to VP and cortical stimulation was less than 1.0 ms. In 19.6% of SI neurons and 41.4% of SII neurons the latency of the response to cortical stimulation was 1.6-4.7 ms shorter than that of the response in the same neuron to VP stimulation. It is supposed that impulses from SI participate significantly in afferent activation of SII neurons.", "contents": "[Responses of neurons of the first and second somatosensory zones of the cortex to peripheral, thalamic and cortical stimulation]. Experiments were performed on cats immobilized with d-tubocurarine or myorelaxin. Neuronal responses were studied in the first somatosensory cortex (SI) to the second somatosensory cortex (SII), ventroposterior nucleus (VP) and contralateral forepaw stimulation. Besides, neuronal responses in SII to SI, VP and contralateral forepaw stimulation were also studied. It was shown that in SII the percentage of neurons excited by afferent volley with two or more synaptic change-overs in the cerebral cortex was larger than in SI. Neurons of SI and SII responded to cortical stimulation ortho- and antidromically, thus confirming the existence of bilateral cortico-cortical connections. Both in SI and SII, PSPs to cortical stimulation were similar in character to PSPs in the same neurons to VP stimulation. In 50.0% of SI neurons and 37.1 of SII neurons the difference in latencies of orthodromic spike potentials to VP and cortical stimulation was less than 1.0 ms. In 19.6% of SI neurons and 41.4% of SII neurons the latency of the response to cortical stimulation was 1.6-4.7 ms shorter than that of the response in the same neuron to VP stimulation. It is supposed that impulses from SI participate significantly in afferent activation of SII neurons.", "PMID": 958523}
{"id": "pubmed23n0032_3105", "title": "[Electrophysiologic study of cortico-hypothalamic interrelationships in cats].", "content": "In acute experiments on cats anesthetized with nembutal and chloralose the projections of different parts of orbito-frontal cortex, basal temporal cortex and hippocampus to hypothalamic nuclei were studied by focal potential recording. It was found that the proreal gyrus has local projections into the latero-dorsal parts of the preoptical region, rostral parts of the forebrain medial bundle, lateral and posterior hypothalamus with mammilary bodies. The orbital gyrus is projected mainly into latero-dorsal parts of the forebrain medial bundle, latero-ventral part of the preoptical region, and the region of lateral and latero-dorsal hypothalamic nuclei. Projections from the orbital gyrus are of a relatively diffuse character. The basal temporal cortex has diffuse projections into the central part of the preoptical region, latero-ventral part of the forebrain medial bundle and lateral mammilary body. No pronounced foci were observed in the hypothalamic structures during stimulation of the hyppocampus, but diffuse projections were found into ventral parts of the preoptical region and ventral regions of the forebrain medial bundle as well as into lateral hypothalamus and lateral mammilary nucleus.", "contents": "[Electrophysiologic study of cortico-hypothalamic interrelationships in cats]. In acute experiments on cats anesthetized with nembutal and chloralose the projections of different parts of orbito-frontal cortex, basal temporal cortex and hippocampus to hypothalamic nuclei were studied by focal potential recording. It was found that the proreal gyrus has local projections into the latero-dorsal parts of the preoptical region, rostral parts of the forebrain medial bundle, lateral and posterior hypothalamus with mammilary bodies. The orbital gyrus is projected mainly into latero-dorsal parts of the forebrain medial bundle, latero-ventral part of the preoptical region, and the region of lateral and latero-dorsal hypothalamic nuclei. Projections from the orbital gyrus are of a relatively diffuse character. The basal temporal cortex has diffuse projections into the central part of the preoptical region, latero-ventral part of the forebrain medial bundle and lateral mammilary body. No pronounced foci were observed in the hypothalamic structures during stimulation of the hyppocampus, but diffuse projections were found into ventral parts of the preoptical region and ventral regions of the forebrain medial bundle as well as into lateral hypothalamus and lateral mammilary nucleus.", "PMID": 958524}
{"id": "pubmed23n0032_3106", "title": "[Organization of cortico-reticulo-spinal connections in the cat].", "content": "Time characteristics of the monosynaptic EPSP evoked by stimulation of the motor cortex in reticulospinal neurons were studied in detail. Negative correlation was revealed between latency duration of EPSP and conduction velocity of the corticobulbar fibres and positive correlation between such characteristics and conduction velocity of axons. Reticulospinal neurons with conduction velocity from 10.8 to 65.0 m/s were activated by fast and slow corticobulbar fibres, but neurons with conduction velocity from 65.0 to 155 m/s were activated only by slow ones. A functional role of different cortico-reticulo-spinal connections is discussed.", "contents": "[Organization of cortico-reticulo-spinal connections in the cat]. Time characteristics of the monosynaptic EPSP evoked by stimulation of the motor cortex in reticulospinal neurons were studied in detail. Negative correlation was revealed between latency duration of EPSP and conduction velocity of the corticobulbar fibres and positive correlation between such characteristics and conduction velocity of axons. Reticulospinal neurons with conduction velocity from 10.8 to 65.0 m/s were activated by fast and slow corticobulbar fibres, but neurons with conduction velocity from 65.0 to 155 m/s were activated only by slow ones. A functional role of different cortico-reticulo-spinal connections is discussed.", "PMID": 958525}
{"id": "pubmed23n0032_3107", "title": "[Reticular structures and reticulospinal pathways participating in initiation and inhibition of spino-bulbo-spinal activity].", "content": "Efferent discharges in intercostal nerves and activity of reticulospinal fibres during stimulation of the brain stem reticular formation, intercostal splanchnic nerves were recorded in cats anesthetized with chloralose (50 mg/kg). It is found that the structures involved in the transmission of spino-bulbo-spinal activity are located in the medial bulbar and pontine reticular formation as well as in its lateral regions. The structures producing long-lasting (300-800 ms) inhibition of spino-bulbo-spinal reflexes are also wide distributed in the brain stem, especially in the pontine region. Recording of activity of single reticulospinal fibres in the ventral (conduction velocity 16-120 m/s) and lateral (17-100 m/s) funiculi during spino-bulbo-spinal reflexes showed that both medial and lateral reticulospinal tracts may be involved into this reflex mechanism. New evidence is obtained that the inhibition of spino-bulbo-spinal reflexes may be located at supraspinal level.", "contents": "[Reticular structures and reticulospinal pathways participating in initiation and inhibition of spino-bulbo-spinal activity]. Efferent discharges in intercostal nerves and activity of reticulospinal fibres during stimulation of the brain stem reticular formation, intercostal splanchnic nerves were recorded in cats anesthetized with chloralose (50 mg/kg). It is found that the structures involved in the transmission of spino-bulbo-spinal activity are located in the medial bulbar and pontine reticular formation as well as in its lateral regions. The structures producing long-lasting (300-800 ms) inhibition of spino-bulbo-spinal reflexes are also wide distributed in the brain stem, especially in the pontine region. Recording of activity of single reticulospinal fibres in the ventral (conduction velocity 16-120 m/s) and lateral (17-100 m/s) funiculi during spino-bulbo-spinal reflexes showed that both medial and lateral reticulospinal tracts may be involved into this reflex mechanism. New evidence is obtained that the inhibition of spino-bulbo-spinal reflexes may be located at supraspinal level.", "PMID": 958526}
{"id": "pubmed23n0032_3108", "title": "[Structure of afferent flow in a cutaneous nerve during painful and non-painful mechanical stimulation of cat skin].", "content": "Using the colliding impulses method, a quantitative characteristic of afferent flows encoding signals from a receptors totality is given. The frequency spectrum of impulsation in the cutaneous nerve, relative quantity of active A-, Adelta- and C-fibres, and their distribution by impulsation frequency under skin stimulation with pins and needles are found. The afferent impulse flow registered in the nerve under pinning of skin is characterized by a high density determined by the quantity of excited fibres and their impulsation frequency. Nonmyelinated fibres directly excited under painful stimulation generate high frequency impulsation due to which the flow density in the nerve increases. The quantity of active myelinated fibres and the frequency of their impulsation under pricking do not increase considerably in comparison with the same characteristics under unnoxious stimulation of cutaneous receptors.", "contents": "[Structure of afferent flow in a cutaneous nerve during painful and non-painful mechanical stimulation of cat skin]. Using the colliding impulses method, a quantitative characteristic of afferent flows encoding signals from a receptors totality is given. The frequency spectrum of impulsation in the cutaneous nerve, relative quantity of active A-, Adelta- and C-fibres, and their distribution by impulsation frequency under skin stimulation with pins and needles are found. The afferent impulse flow registered in the nerve under pinning of skin is characterized by a high density determined by the quantity of excited fibres and their impulsation frequency. Nonmyelinated fibres directly excited under painful stimulation generate high frequency impulsation due to which the flow density in the nerve increases. The quantity of active myelinated fibres and the frequency of their impulsation under pricking do not increase considerably in comparison with the same characteristics under unnoxious stimulation of cutaneous receptors.", "PMID": 958527}
{"id": "pubmed23n0032_3109", "title": "[Effect of calcium ions on potential-dependent potassium channels in the membrane of the soma of giant mollusc neurons].", "content": "The delayed and fast outward currents in the somatic membrane of mollusc giant neurons were studied under voltage-clamp conditions in normal (10 mM) and low-Ca (I mM) saline. At pH 7.2 a tenfold decrease in calcium concentration in the peak conductance vs membrane potential curves for delayed and fast outward currents were shifted along the voltage axis by 10 mV. The shift along voltage axis of the inactivation curve for fast outward current under similar conditions was the same. At pH 9 the shift for a tenfold decrease in calcium concentration was 20 mV. Such an increase in the effect of calcium concentration change at pH 9 is probably due to the increase in the negative charge density in the vicinity of the potassium pathways. The dependence of the rate constants of inactivation alphab and betab for fast outward current upon the membrane potential was studied in solutions with different calcium concentration. The expressions for their calculation were obtained.", "contents": "[Effect of calcium ions on potential-dependent potassium channels in the membrane of the soma of giant mollusc neurons]. The delayed and fast outward currents in the somatic membrane of mollusc giant neurons were studied under voltage-clamp conditions in normal (10 mM) and low-Ca (I mM) saline. At pH 7.2 a tenfold decrease in calcium concentration in the peak conductance vs membrane potential curves for delayed and fast outward currents were shifted along the voltage axis by 10 mV. The shift along voltage axis of the inactivation curve for fast outward current under similar conditions was the same. At pH 9 the shift for a tenfold decrease in calcium concentration was 20 mV. Such an increase in the effect of calcium concentration change at pH 9 is probably due to the increase in the negative charge density in the vicinity of the potassium pathways. The dependence of the rate constants of inactivation alphab and betab for fast outward current upon the membrane potential was studied in solutions with different calcium concentration. The expressions for their calculation were obtained.", "PMID": 958528}
{"id": "pubmed23n0032_3110", "title": "[Displacement currents of the sodium channels of the membranes of Ranvier's nodes].", "content": "A method to record the displacement current in nodal membrane is described and results obtained on nerve fibres from the frog Rana ridibunda are presented. This current is shown to be due to displacement of charges from an initial state, associated with a large negative membrane potential, to a final state. The dependence of the charge displacement on the membrane potential and degree of inactivation of the sodium channels suggests that the displacement current observed in these experiments is associated with activation of m-gates of the sodium channels. The density of the sodium channels in nodal membrane of Rana ridibunda determined from the density of the charge displaced is about 5000 channels/mum2.", "contents": "[Displacement currents of the sodium channels of the membranes of Ranvier's nodes]. A method to record the displacement current in nodal membrane is described and results obtained on nerve fibres from the frog Rana ridibunda are presented. This current is shown to be due to displacement of charges from an initial state, associated with a large negative membrane potential, to a final state. The dependence of the charge displacement on the membrane potential and degree of inactivation of the sodium channels suggests that the displacement current observed in these experiments is associated with activation of m-gates of the sodium channels. The density of the sodium channels in nodal membrane of Rana ridibunda determined from the density of the charge displaced is about 5000 channels/mum2.", "PMID": 958529}
{"id": "pubmed23n0032_3111", "title": "[Decrease in the maximum sodium permeability and slow sodium inactivation in Ranvier's nodes treated with trimecaine].", "content": "The effect of local anesthetic trimecaine on sodium permeability was studied in voltage clamped nerve fibres of the frog. Trimecaine affected PNa by means of two different mechanisms: potential independent block (reduction of PNa) and slow inactivation imposed by prolonged depolarization. Trimecaine induced slow inactivation appeared to be qualitatively similar to that produced by externally applied procaine or high external potassium. According to dose-effect curves one molecule of drug per channel reaction is involved for both mechanisms. The drug molecule has greater affinity for the receptor site responsible for slow inactivation.", "contents": "[Decrease in the maximum sodium permeability and slow sodium inactivation in Ranvier's nodes treated with trimecaine]. The effect of local anesthetic trimecaine on sodium permeability was studied in voltage clamped nerve fibres of the frog. Trimecaine affected PNa by means of two different mechanisms: potential independent block (reduction of PNa) and slow inactivation imposed by prolonged depolarization. Trimecaine induced slow inactivation appeared to be qualitatively similar to that produced by externally applied procaine or high external potassium. According to dose-effect curves one molecule of drug per channel reaction is involved for both mechanisms. The drug molecule has greater affinity for the receptor site responsible for slow inactivation.", "PMID": 958530}
{"id": "pubmed23n0032_3112", "title": "Preliminary analysis of failures in the radiotherapy of uterine cervix cancer with a dynamic dose-fractionation in combination with breathing oxygen at ambient pressure.", "content": "Complementary to a previous preliminary analysis of the results obtained in treating head and neck tumors, this paper summarizes observations in testing uterine cervix cancers with a dynamic dose-fractionation regime using Cobalt 60 radiation in combination with oxygen breathing at ambient pressure. A total of 30 cases in different stages are reviewed which had no treatment prior to radiotherapy. In agreement with previous observations, normal tissue reactions were decreased in comparison to those seen after conventional dose-fractionation. Exophytic type of tumors with relatively good vascularization appears to react better to the treatment then less vascularized endophytic tumors. For the evaluation of the possible role of the dynamic fractionation in these results collection of further data is needed.", "contents": "Preliminary analysis of failures in the radiotherapy of uterine cervix cancer with a dynamic dose-fractionation in combination with breathing oxygen at ambient pressure. Complementary to a previous preliminary analysis of the results obtained in treating head and neck tumors, this paper summarizes observations in testing uterine cervix cancers with a dynamic dose-fractionation regime using Cobalt 60 radiation in combination with oxygen breathing at ambient pressure. A total of 30 cases in different stages are reviewed which had no treatment prior to radiotherapy. In agreement with previous observations, normal tissue reactions were decreased in comparison to those seen after conventional dose-fractionation. Exophytic type of tumors with relatively good vascularization appears to react better to the treatment then less vascularized endophytic tumors. For the evaluation of the possible role of the dynamic fractionation in these results collection of further data is needed.", "PMID": 958531}
{"id": "pubmed23n0032_3113", "title": "Peripheral blood lymphocyte counts and survival in breast cancer.", "content": "The lymphocytes in the peripheral blood of patients with breast cancer were studied. Peripheral blood lymphocyte counts were found to be significantly lower in the short-survivors when compared with the long survivors. Lymphocyte count may be a host factor, that influences survival in breast cancer.", "contents": "Peripheral blood lymphocyte counts and survival in breast cancer. The lymphocytes in the peripheral blood of patients with breast cancer were studied. Peripheral blood lymphocyte counts were found to be significantly lower in the short-survivors when compared with the long survivors. Lymphocyte count may be a host factor, that influences survival in breast cancer.", "PMID": 958532}
{"id": "pubmed23n0032_3114", "title": "Chemotherapy of advanced malignant lymphomas comparative evaluation of results with single agent and combination therapy.", "content": "86 patients with advanced malignant lymphomas (stage III and IV) were treated either with Vinblastine given as a single agent (49 cases) or with combination chemotherapy MOPP or COP (37 cases). In both, Hodgkin's disease and non-Hodgkin's lymphomas, induction of remission, relaps-free survival and prognostic significance of initial clinical stage were evaluated, in relation to the therapeutic modality. In patients with Hodgkin's disease, combination chemotherapy was found to produce significantly more durable remissions, when compared with single agent therapy. However, difference in the response rate, which was found to be more effective following the combination chemotherapy, was not statistically significant. In patients with non-Hodgkin's lymphomas overall lower response was recorded, regardless of the therapy applied. In all patients, better  response rate and longer lasting remissions correlated with initial stage III.", "contents": "Chemotherapy of advanced malignant lymphomas comparative evaluation of results with single agent and combination therapy. 86 patients with advanced malignant lymphomas (stage III and IV) were treated either with Vinblastine given as a single agent (49 cases) or with combination chemotherapy MOPP or COP (37 cases). In both, Hodgkin's disease and non-Hodgkin's lymphomas, induction of remission, relaps-free survival and prognostic significance of initial clinical stage were evaluated, in relation to the therapeutic modality. In patients with Hodgkin's disease, combination chemotherapy was found to produce significantly more durable remissions, when compared with single agent therapy. However, difference in the response rate, which was found to be more effective following the combination chemotherapy, was not statistically significant. In patients with non-Hodgkin's lymphomas overall lower response was recorded, regardless of the therapy applied. In all patients, better  response rate and longer lasting remissions correlated with initial stage III.", "PMID": 958533}
{"id": "pubmed23n0032_3115", "title": "The metastatic spread of kidney and prostate cancers in man.", "content": "This research was performed on 66 autopsy reports of kidney cancers and 58 autopsy reports of prostate cancers. Two alternative hypotheses were tested, i.e. whether metastases spread at random from the primary tumor throughout the body or whether the metastatic spread occurs in steps, referred to as a cascade spread, requiring one or more disseminating sites. It was found that in cancer of the prostate two disseminating sites exist, i.e. bones and lungs. Metastases from the primary tumor appear first in the skeleton and then in the lungs, not vice versa. In cancer of the kidney, the lungs are the major disseminating site. The lymphatic areas did not seem to play a major role in the dissemination of both cancers; on the contrary, the presence of metastases in the lymph nodes seem to depend on the presence of carcinomatous cells in key sites, i.e. the skeleton and the lungs. These results, however, should be interpreted with caution, as explained in the text. Considerations were proposed for future research.", "contents": "The metastatic spread of kidney and prostate cancers in man. This research was performed on 66 autopsy reports of kidney cancers and 58 autopsy reports of prostate cancers. Two alternative hypotheses were tested, i.e. whether metastases spread at random from the primary tumor throughout the body or whether the metastatic spread occurs in steps, referred to as a cascade spread, requiring one or more disseminating sites. It was found that in cancer of the prostate two disseminating sites exist, i.e. bones and lungs. Metastases from the primary tumor appear first in the skeleton and then in the lungs, not vice versa. In cancer of the kidney, the lungs are the major disseminating site. The lymphatic areas did not seem to play a major role in the dissemination of both cancers; on the contrary, the presence of metastases in the lymph nodes seem to depend on the presence of carcinomatous cells in key sites, i.e. the skeleton and the lungs. These results, however, should be interpreted with caution, as explained in the text. Considerations were proposed for future research.", "PMID": 958534}
{"id": "pubmed23n0032_3116", "title": "An assay for selecting high risk population for gastric cancer by studying environmental factors.", "content": "Gastric cancer mortality incidence data registered in two different areas of Eastern Transylvania (Roumania) were reported related to 325,000 inhabitants from the period of 1951-1972. The findings were compared to some geographical environmental factors deriving from an area of 13,300 km with 905,700 inhabitants. A 2-3.5 times larger incidence of gastric cancer (75-140 per 100,000/year) was found in some selected geographical areas of the intermontane depressions of Gheorgheni and Ciuc in comparison to hilly area of Transylvanian Tableland. The difference might be explained by some unknown environmental gastric cancer risk factors. Of the natural factors, the presence of magmatic substrata shows a significant degree of correlation. The main pedological factor seems to be badly drained pseudoglyied podzolic and peaty soils of low pH and high content of organic matter. Sofs drinking waters also may be involved as risk factor. High altitude, cold climate determining a restricted assortiment of cultivated plants, the successive production of vegetal and animal food on the same soil for livelong periods and several generations, especially in isolated rural areas, seem to represent gastric cancer risk factors. According to authors' opinion a survey of the high-risk population selected on the basis of the environmental factors, especially of the persons suffering from gastric disorders considered today possible precursors of gastric cancer, may offer some progress in detecting early gastric malignancy in the future.", "contents": "An assay for selecting high risk population for gastric cancer by studying environmental factors. Gastric cancer mortality incidence data registered in two different areas of Eastern Transylvania (Roumania) were reported related to 325,000 inhabitants from the period of 1951-1972. The findings were compared to some geographical environmental factors deriving from an area of 13,300 km with 905,700 inhabitants. A 2-3.5 times larger incidence of gastric cancer (75-140 per 100,000/year) was found in some selected geographical areas of the intermontane depressions of Gheorgheni and Ciuc in comparison to hilly area of Transylvanian Tableland. The difference might be explained by some unknown environmental gastric cancer risk factors. Of the natural factors, the presence of magmatic substrata shows a significant degree of correlation. The main pedological factor seems to be badly drained pseudoglyied podzolic and peaty soils of low pH and high content of organic matter. Sofs drinking waters also may be involved as risk factor. High altitude, cold climate determining a restricted assortiment of cultivated plants, the successive production of vegetal and animal food on the same soil for livelong periods and several generations, especially in isolated rural areas, seem to represent gastric cancer risk factors. According to authors' opinion a survey of the high-risk population selected on the basis of the environmental factors, especially of the persons suffering from gastric disorders considered today possible precursors of gastric cancer, may offer some progress in detecting early gastric malignancy in the future.", "PMID": 958535}
{"id": "pubmed23n0032_3117", "title": "Effect of subarachnoid haemorrhage on micro-circulation in hypothalamus and brain stem of dogs.", "content": "In order to investigate the relationship between cerebral vasospasm and microvasculature in the hypothalamus and brain stem, colloidal carbon was infused into the vertebral artery at various time intervals after experimental subarachnoid haemorrhage in dogs. Experiments which demonstrated vasospasm on angiogram were always accompanied by ischaemic changes in serial sections taken from the anterior hypothalamus to the brain stem. However, when it was demonstrated by angiography that the vasospasm had disappeared, the micro-circulation was restored to normal. Electron microscopy of the hypothalamus 48 hours and one week after subarachnoid haemorrhage, demonstrated swelling of the endothelial cells, enlargement of the perivascular glia and increase in number of the pinocytic vesicles in the cytoplasm, thus showing vasogenic oedema in this area. It is assumed that in addition to the vasogenic substance in extravasated blood, changes in irritability of cerebral vessels through the vasomotor pathways in the hypothalamus and brain stem might play an important role in the production of cerebral vasospasm.", "contents": "Effect of subarachnoid haemorrhage on micro-circulation in hypothalamus and brain stem of dogs. In order to investigate the relationship between cerebral vasospasm and microvasculature in the hypothalamus and brain stem, colloidal carbon was infused into the vertebral artery at various time intervals after experimental subarachnoid haemorrhage in dogs. Experiments which demonstrated vasospasm on angiogram were always accompanied by ischaemic changes in serial sections taken from the anterior hypothalamus to the brain stem. However, when it was demonstrated by angiography that the vasospasm had disappeared, the micro-circulation was restored to normal. Electron microscopy of the hypothalamus 48 hours and one week after subarachnoid haemorrhage, demonstrated swelling of the endothelial cells, enlargement of the perivascular glia and increase in number of the pinocytic vesicles in the cytoplasm, thus showing vasogenic oedema in this area. It is assumed that in addition to the vasogenic substance in extravasated blood, changes in irritability of cerebral vessels through the vasomotor pathways in the hypothalamus and brain stem might play an important role in the production of cerebral vasospasm.", "PMID": 958560}
{"id": "pubmed23n0032_3118", "title": "Role of the pneumo-encephalogram in the diagnosis of spontaneous subarachnoid haemorrhage.", "content": "The authors have collected eighteen cases of primary intraventricular lesions revealed by a subarachnoid haemorrhage. Among these cases, the most important are the choroid plexus papillomas followed by ependymomas; some cases have no precise histological interpretation. Generally speaking, cerebral arteriography gives only indirect evidence of ventricular dilation and thus does not confirm the presence of a lesion. On the contrary, the pneumoencephalogram is consistantly positive, but does not give the differential diagnosis between intraventricular tumours and primary intraventricular haemorrhages. This test should therefore be given the same importance as spinal cord arteriography in the exploration of subarachnoid haemorrhages with negative cerebral angiograms.", "contents": "Role of the pneumo-encephalogram in the diagnosis of spontaneous subarachnoid haemorrhage. The authors have collected eighteen cases of primary intraventricular lesions revealed by a subarachnoid haemorrhage. Among these cases, the most important are the choroid plexus papillomas followed by ependymomas; some cases have no precise histological interpretation. Generally speaking, cerebral arteriography gives only indirect evidence of ventricular dilation and thus does not confirm the presence of a lesion. On the contrary, the pneumoencephalogram is consistantly positive, but does not give the differential diagnosis between intraventricular tumours and primary intraventricular haemorrhages. This test should therefore be given the same importance as spinal cord arteriography in the exploration of subarachnoid haemorrhages with negative cerebral angiograms.", "PMID": 958561}
{"id": "pubmed23n0032_3119", "title": "Acute spontaneous volume fluctuation in the brain. A contribution to the interpretation of plateau waves. Results of CSF measurements with ventricular drainage.", "content": "Primarily the theoretical background of the relation between the intracranial pressure and the volume is discussed. From the definite relationship between volume and pressure it is deduced that with the appropriate equipment intracranial volume changes may be measured instead of the I.C.P. The exact measurement of the volume of CSF drained in relation to the drainage time enable us to differentiate disturbances of the C.S.F. circulation in patients. Furthermore we were able to correlate \"plateau waves\" with certain amounts of volume fluctuations; these were shown in amounts of drained C.S.F. between 16 and 32 ml. Some scientific aspects of this new method and possible results are pointed out.", "contents": "Acute spontaneous volume fluctuation in the brain. A contribution to the interpretation of plateau waves. Results of CSF measurements with ventricular drainage. Primarily the theoretical background of the relation between the intracranial pressure and the volume is discussed. From the definite relationship between volume and pressure it is deduced that with the appropriate equipment intracranial volume changes may be measured instead of the I.C.P. The exact measurement of the volume of CSF drained in relation to the drainage time enable us to differentiate disturbances of the C.S.F. circulation in patients. Furthermore we were able to correlate \"plateau waves\" with certain amounts of volume fluctuations; these were shown in amounts of drained C.S.F. between 16 and 32 ml. Some scientific aspects of this new method and possible results are pointed out.", "PMID": 958562}
{"id": "pubmed23n0032_3120", "title": "Spinal fluid lysozyme in the diagnosis of central nervous system tumours.", "content": "C.S.F. lysozyme concentrations were determined in 24 normal subjects and 14 patients with a variety of neurologic diseases. We found absent or very low activity (below 0-5 mg/ml, in 4 out of 24) in normal individuals. Patients with tumours and other neurological diseases had high lysozyme activity in C.S.F. Our findings suggest that lysozyme is not an accurate indicator of the presence of neoplastic disease as suggested by other investigators.", "contents": "Spinal fluid lysozyme in the diagnosis of central nervous system tumours. C.S.F. lysozyme concentrations were determined in 24 normal subjects and 14 patients with a variety of neurologic diseases. We found absent or very low activity (below 0-5 mg/ml, in 4 out of 24) in normal individuals. Patients with tumours and other neurological diseases had high lysozyme activity in C.S.F. Our findings suggest that lysozyme is not an accurate indicator of the presence of neoplastic disease as suggested by other investigators.", "PMID": 958563}
{"id": "pubmed23n0032_3121", "title": "[Functional posterior radiculotomy, in the treatment of cerebral spasticity. peroperative electric stimulation of posterior roots and its use in the choice of the roots to be sectioned].", "content": "The authors present a modification of Foerster's Rhizotomy for the treatment of  spasticity in cerebral palsy: functional posterior rhizotomy. The selection of the roots/rootlesses to be sectioned, is accomplished on functional data, based upon the analysis of the reflex responses to the intraoperative lumbar dorsal roots stimulation. With this method it is possible to selectively interfere with the pathological circuits responsible for hypertonia, saving proprioceptive afferences necessary for motor reeducation. The clinical results on hypertonia are the same as for total or partial rhizotomies, but sides effects (ataxia, hypotonia) are considerably reduced.", "contents": "[Functional posterior radiculotomy, in the treatment of cerebral spasticity. peroperative electric stimulation of posterior roots and its use in the choice of the roots to be sectioned]. The authors present a modification of Foerster's Rhizotomy for the treatment of  spasticity in cerebral palsy: functional posterior rhizotomy. The selection of the roots/rootlesses to be sectioned, is accomplished on functional data, based upon the analysis of the reflex responses to the intraoperative lumbar dorsal roots stimulation. With this method it is possible to selectively interfere with the pathological circuits responsible for hypertonia, saving proprioceptive afferences necessary for motor reeducation. The clinical results on hypertonia are the same as for total or partial rhizotomies, but sides effects (ataxia, hypotonia) are considerably reduced.", "PMID": 958564}
{"id": "pubmed23n0032_3122", "title": "[Success and failure of the ventriculoperitoneal shunt in the treatment of hydrocephalus].", "content": "In ventriculoperitoneal shunting, the intraperitoneal location of the drainage site can be an additional cause of failure. In our series of 70 ventriculoperitoneal shunts, performed in infants and adults for treatment of hydrocephalus from various cause and of various type, failures of this kind manifested themselves usually early in the postoperative period either as a reflux of liquid, not attributable to the operative technique, into the subcutaneous pericatheter tract, or as a reappearance of the intracranial hypertension, without faulty functioning of the ventricular drain. The total success rate was 61% (43/70) but the age of the patient made a clear difference. In patients aged less than 5 months, we observed 11 successes in 33 cases (33%), in those aged less than 1 year, 14 successes in 36 (39%); patients with myelomeninogocele, wherein the shunting was usually done during the first month of life,  fared badly; the role of age seems herein predominant. In patients aged more than 1 year, 29 successes in 34 cases (85%) were obtained; results were notably good in nearly all hydrocephalus of tumoral origin. We think the possible role of the \"tightness\" of the hydrocephalus has to be invoked as also the possibility of spontaneous remission. Ventriculoperitoneal shunting, devoid of the serious complications of ventriculocardiac shunting, is a simple and effective procedure in many cases of hydrocephalus. Means for more accurate prediction of the chances of success in the individual case are needed.", "contents": "[Success and failure of the ventriculoperitoneal shunt in the treatment of hydrocephalus]. In ventriculoperitoneal shunting, the intraperitoneal location of the drainage site can be an additional cause of failure. In our series of 70 ventriculoperitoneal shunts, performed in infants and adults for treatment of hydrocephalus from various cause and of various type, failures of this kind manifested themselves usually early in the postoperative period either as a reflux of liquid, not attributable to the operative technique, into the subcutaneous pericatheter tract, or as a reappearance of the intracranial hypertension, without faulty functioning of the ventricular drain. The total success rate was 61% (43/70) but the age of the patient made a clear difference. In patients aged less than 5 months, we observed 11 successes in 33 cases (33%), in those aged less than 1 year, 14 successes in 36 (39%); patients with myelomeninogocele, wherein the shunting was usually done during the first month of life,  fared badly; the role of age seems herein predominant. In patients aged more than 1 year, 29 successes in 34 cases (85%) were obtained; results were notably good in nearly all hydrocephalus of tumoral origin. We think the possible role of the \"tightness\" of the hydrocephalus has to be invoked as also the possibility of spontaneous remission. Ventriculoperitoneal shunting, devoid of the serious complications of ventriculocardiac shunting, is a simple and effective procedure in many cases of hydrocephalus. Means for more accurate prediction of the chances of success in the individual case are needed.", "PMID": 958565}
{"id": "pubmed23n0032_3123", "title": "[Intraspinal meningiomas. A series of 60 cases].", "content": "The authors present and compare two consecutive series of spinal meningioma, a total of 60 cases. From the aetiological point of view, the meningioma were more frequent than neurinoma. This study confirms its predominance in the female after the fifth decade. A traumatic factor was sometimes implicated. Anatomically, its localisation predominated in the dorsal region (78.3 p. cent), specially in the first three vertebral segments, and most commonly laterally placed. The strictly epidural meningioma, the multiplicity and the association with a neurinoma were less frequent. On the clinical level, the comparison between the two series illustrated a considerable diminution of the pre-operative evolution of the disease, taking into consideration the existence of spinal deficit less apparent in patients operated after 1963. 54.5 p. cent presented at diagnosis, a total or partial impotence against 81.5 p. cent in the old series. Among the laboratory examinations, the C.D.F. study confirmed slight or even absent cyto-albuminous dissociation, which contrasted markedly with the figures obtained in cases of neurinoma. Plain spinal radiography was usually negative. The radio-opaque myelography revealed a block, sometimes a partial one, very suggestive when bossulated, but which might sometimes mimic a neurinoma or even an epidural lesion. The presence of an arachnoid cyst may give a false level. Surgery was always indicated, regardless of the patients' age. The tumour should be completly removed in a piece-meal fashion with the preservation of the radiculo-medullary elements, which was facilitated by the use of the microscope. To prevent a recurrence, the base of the tumour should be well coagulated or resected followed by a plastic reconstruction of the dura mater. The mortality rate including the late mortality has diminished from 24 p. cent to 11 p. cent. This is essentially due to decubitus in patients who did not improve. The percentage of complete functional recovery rose from 40 p. cent to 66 p. cent of those who survived. The percentage of recovery with sequellae compatible with an autonomous physical activity was stable. The percentage of completly handicaped survivals fell from 28 p. cent to 5 p. cent. The authors conclude that the prognosis for patients with intraspinal menigioma has improved, however the diagnosis is still difficult in the old subject, and there is a serious risk to life when the lesion is in the cervical region.", "contents": "[Intraspinal meningiomas. A series of 60 cases]. The authors present and compare two consecutive series of spinal meningioma, a total of 60 cases. From the aetiological point of view, the meningioma were more frequent than neurinoma. This study confirms its predominance in the female after the fifth decade. A traumatic factor was sometimes implicated. Anatomically, its localisation predominated in the dorsal region (78.3 p. cent), specially in the first three vertebral segments, and most commonly laterally placed. The strictly epidural meningioma, the multiplicity and the association with a neurinoma were less frequent. On the clinical level, the comparison between the two series illustrated a considerable diminution of the pre-operative evolution of the disease, taking into consideration the existence of spinal deficit less apparent in patients operated after 1963. 54.5 p. cent presented at diagnosis, a total or partial impotence against 81.5 p. cent in the old series. Among the laboratory examinations, the C.D.F. study confirmed slight or even absent cyto-albuminous dissociation, which contrasted markedly with the figures obtained in cases of neurinoma. Plain spinal radiography was usually negative. The radio-opaque myelography revealed a block, sometimes a partial one, very suggestive when bossulated, but which might sometimes mimic a neurinoma or even an epidural lesion. The presence of an arachnoid cyst may give a false level. Surgery was always indicated, regardless of the patients' age. The tumour should be completly removed in a piece-meal fashion with the preservation of the radiculo-medullary elements, which was facilitated by the use of the microscope. To prevent a recurrence, the base of the tumour should be well coagulated or resected followed by a plastic reconstruction of the dura mater. The mortality rate including the late mortality has diminished from 24 p. cent to 11 p. cent. This is essentially due to decubitus in patients who did not improve. The percentage of complete functional recovery rose from 40 p. cent to 66 p. cent of those who survived. The percentage of recovery with sequellae compatible with an autonomous physical activity was stable. The percentage of completly handicaped survivals fell from 28 p. cent to 5 p. cent. The authors conclude that the prognosis for patients with intraspinal menigioma has improved, however the diagnosis is still difficult in the old subject, and there is a serious risk to life when the lesion is in the cervical region.", "PMID": 958566}
{"id": "pubmed23n0032_3124", "title": "[Value of the intracranial pressue measurement in the craniosynostoses detected after the age of 1 year].", "content": "Notwithstanding the fact that there is a general agreement on the necessity of surgery in the first year of life in craniosynostosis, the problem is more difficult for older children. Deterioration of clinical status is, in general, in relation with an increase in intracranial pressure (ICP), which may happen abruptly following different causes (slight head injury, for example). The purpose of this study was to determine, in different varieties of synostosis, if a high intracranial pressure could exist without clinical signs and consequently, if a surgical opening of the sutures would be necessary. IPC has been measured through a ventricular catheter connected with a transducer and recorder for 24 hours. In half of the cases (11 out of 22) a high ICP (above 20 mmHg) was recorded either permanently or during sleep. This increase in ICP should lead to a surgical decompression, event without clinical signs. On the other hand, psychomotor retardation, abnormal EEG, increased digitation should not be considered as an indication for surgical treatment in cases with normal ICP. The measurement of decreased ICP after operation on a long term basis would be a great value.", "contents": "[Value of the intracranial pressue measurement in the craniosynostoses detected after the age of 1 year]. Notwithstanding the fact that there is a general agreement on the necessity of surgery in the first year of life in craniosynostosis, the problem is more difficult for older children. Deterioration of clinical status is, in general, in relation with an increase in intracranial pressure (ICP), which may happen abruptly following different causes (slight head injury, for example). The purpose of this study was to determine, in different varieties of synostosis, if a high intracranial pressure could exist without clinical signs and consequently, if a surgical opening of the sutures would be necessary. IPC has been measured through a ventricular catheter connected with a transducer and recorder for 24 hours. In half of the cases (11 out of 22) a high ICP (above 20 mmHg) was recorded either permanently or during sleep. This increase in ICP should lead to a surgical decompression, event without clinical signs. On the other hand, psychomotor retardation, abnormal EEG, increased digitation should not be considered as an indication for surgical treatment in cases with normal ICP. The measurement of decreased ICP after operation on a long term basis would be a great value.", "PMID": 958567}
{"id": "pubmed23n0032_3125", "title": "[Comparative in vitro action of various antimitotic agents on cell lines established from human brain neoplasms].", "content": "The effects of six chemotherapeutic drugs currently used in human gliomas treatment were tested with an in vitro cytotoxicity test. Out of six, VM 26 proved to be the most potent against tumor cells along with a moderate cytotoxicity against normal brain or embryonnic human cells. This specificity did not seem to be related to differences in cellular growth rates.", "contents": "[Comparative in vitro action of various antimitotic agents on cell lines established from human brain neoplasms]. The effects of six chemotherapeutic drugs currently used in human gliomas treatment were tested with an in vitro cytotoxicity test. Out of six, VM 26 proved to be the most potent against tumor cells along with a moderate cytotoxicity against normal brain or embryonnic human cells. This specificity did not seem to be related to differences in cellular growth rates.", "PMID": 958568}
{"id": "pubmed23n0032_3126", "title": "[Giant aneurysm of the sylvian artery in a 4-year old child (exeresis, remission)].", "content": "The AA. describe a case of a giant aneurysm 6 centimeters in diameter in a child of 4 years old. The lesion was made evident on the occasion of an hemorrhage that has caused an insular hematoma. The patient has undergone an operation with favourable outcome. The lack of neurologic symtomatology until the moment of the hemorrhage lead the AA. to think that this lesion was of the notables proportions from the moment of birth, so that the cerebral parenchyma was modeled on it. Unlike others cases in litterature recogniset, the wall of the aneurysmatic bag did not present fibrotics thickenings, and besides the blood-clot included in it seemed histologicaly of recent formation.", "contents": "[Giant aneurysm of the sylvian artery in a 4-year old child (exeresis, remission)]. The AA. describe a case of a giant aneurysm 6 centimeters in diameter in a child of 4 years old. The lesion was made evident on the occasion of an hemorrhage that has caused an insular hematoma. The patient has undergone an operation with favourable outcome. The lack of neurologic symtomatology until the moment of the hemorrhage lead the AA. to think that this lesion was of the notables proportions from the moment of birth, so that the cerebral parenchyma was modeled on it. Unlike others cases in litterature recogniset, the wall of the aneurysmatic bag did not present fibrotics thickenings, and besides the blood-clot included in it seemed histologicaly of recent formation.", "PMID": 958569}
{"id": "pubmed23n0032_3127", "title": "[Sensory organization of the reticular formation: Specific integrative activity].", "content": "On the basis of personal investigations including 1) electrophysiological methods (EEG, averaged evoked potentials), 2) behavior methods, 3) destruction of various parts of the brain (mesencephalic reticular formation, auditory pathways) and 4) pharmacological methods, conducted in the years 1953-1974 the author put forward a concept of sensory organization of reticular formation postulating existence of special systems in the reticular formation differing in their relative physiological and chemical specificity. This relative specificity is, in this concept, the ability of the so-called non-specific neurons in the reticular formation to be included under certain experimental conditions into specific functional systems. The author suggests the term \"paraspecific systems\" for designating these relatively specific systems. It seems that these systems determine the integrative role of reticular formation in the process of learning. In contrast to this important role the reticular formation is without any greater importance for the process of memory storage. The dynamic and relatively specific functional organization of reticular formation provides potential possibilities of its utilization in the processes of reeducation or rehabilitation in central nervous system damage and in pharmacological treatment of psychic disturbances.", "contents": "[Sensory organization of the reticular formation: Specific integrative activity]. On the basis of personal investigations including 1) electrophysiological methods (EEG, averaged evoked potentials), 2) behavior methods, 3) destruction of various parts of the brain (mesencephalic reticular formation, auditory pathways) and 4) pharmacological methods, conducted in the years 1953-1974 the author put forward a concept of sensory organization of reticular formation postulating existence of special systems in the reticular formation differing in their relative physiological and chemical specificity. This relative specificity is, in this concept, the ability of the so-called non-specific neurons in the reticular formation to be included under certain experimental conditions into specific functional systems. The author suggests the term \"paraspecific systems\" for designating these relatively specific systems. It seems that these systems determine the integrative role of reticular formation in the process of learning. In contrast to this important role the reticular formation is without any greater importance for the process of memory storage. The dynamic and relatively specific functional organization of reticular formation provides potential possibilities of its utilization in the processes of reeducation or rehabilitation in central nervous system damage and in pharmacological treatment of psychic disturbances.", "PMID": 958570}
{"id": "pubmed23n0032_3128", "title": "[Gasometric studies of arterial blood in patients with stroke].", "content": "Gasometric determinations of pCO2, pO2, HCO3 and SO2 were performed in arterial blood in 100 patients with brain stroke. The following conclusions have been drawn. 1. In 2/3 of cases of brain stroke decreased partial oxygen pressure and in 1/3 of cases acid-base equilibrium disturbances are observed. 2. Hypoxia occurs in brain stroke mainly in patients with consciousness disturbances, which suggests that impaired airways patency plays a role in the pathomechanism of this disturbance. 3. Acid-base equilibrium disturbances in cases of brain stroke have in most patients the character of respiratory alkalosis which seems to be due to hyperventilation determined by central nervous mechanisms as well as to hypoxia caused by insufficient patency of airways. 4. Gasometric investigations of arterial blood in brain stroke are important for establishing the degree of homeostasis disturbances and for choice of methods of therapeutic management.", "contents": "[Gasometric studies of arterial blood in patients with stroke]. Gasometric determinations of pCO2, pO2, HCO3 and SO2 were performed in arterial blood in 100 patients with brain stroke. The following conclusions have been drawn. 1. In 2/3 of cases of brain stroke decreased partial oxygen pressure and in 1/3 of cases acid-base equilibrium disturbances are observed. 2. Hypoxia occurs in brain stroke mainly in patients with consciousness disturbances, which suggests that impaired airways patency plays a role in the pathomechanism of this disturbance. 3. Acid-base equilibrium disturbances in cases of brain stroke have in most patients the character of respiratory alkalosis which seems to be due to hyperventilation determined by central nervous mechanisms as well as to hypoxia caused by insufficient patency of airways. 4. Gasometric investigations of arterial blood in brain stroke are important for establishing the degree of homeostasis disturbances and for choice of methods of therapeutic management.", "PMID": 958571}
{"id": "pubmed23n0032_3129", "title": "[Usefulness of electromyographic examinations of paravertebral muscles in patients with lumbosacral root compression syndrome].", "content": "The author studied electromyographic findings and surgical exploration results in 37 women and 63 men operated upon for nucleus pulposus prolapse. EMG investigations of paravertebral muscles were found to be useful in the diagnosis of motor root lesions. A correlation was found between the size of prolapse and the area of denervation potentials in paravertebral muscles. On the other hand, it was found that these investigations do not indicate accurately the level of nucleus pulposus prolapse.", "contents": "[Usefulness of electromyographic examinations of paravertebral muscles in patients with lumbosacral root compression syndrome]. The author studied electromyographic findings and surgical exploration results in 37 women and 63 men operated upon for nucleus pulposus prolapse. EMG investigations of paravertebral muscles were found to be useful in the diagnosis of motor root lesions. A correlation was found between the size of prolapse and the area of denervation potentials in paravertebral muscles. On the other hand, it was found that these investigations do not indicate accurately the level of nucleus pulposus prolapse.", "PMID": 958574}
{"id": "pubmed23n0032_3130", "title": "[Fatigability of stapedius muscle in myasthenia].", "content": "The fatiguability of m. stupedius was studied in 20 patients with myasthenia and 16 controls determining the reflex of this muscle by means of electroacoustic bridge. In half of the patients group the fatiguability of stapedius muscle was increased and in some cases the reflex threshold of the muscle was raised. The authors suggest continuation of these investigations especially for determination of the clinical importance of these observations.", "contents": "[Fatigability of stapedius muscle in myasthenia]. The fatiguability of m. stupedius was studied in 20 patients with myasthenia and 16 controls determining the reflex of this muscle by means of electroacoustic bridge. In half of the patients group the fatiguability of stapedius muscle was increased and in some cases the reflex threshold of the muscle was raised. The authors suggest continuation of these investigations especially for determination of the clinical importance of these observations.", "PMID": 958573}
{"id": "pubmed23n0032_3131", "title": "[Our modification of ergograph for studying muscle fatigability in myasthenia].", "content": "The authors designed and made an ergograph of own modification and elaborated normal values of fatiguability half-time on the basis of 51 healthy subjects comparing the results with those in a group of 41 myasthenics subjected to thymectomy. The fatiguability half-time was determined in groups of muscles in the hand, axilla and popliteal area and masseters before and 14 days after thymectomy. The ergographic record was obtained without application of drugs as well as at the peak of activity of drugs and at the peak of activity of those after 5 mg Tensilon dose. The investigation made it possible to determine the muscle power before and after tymectomy. The Tensioln test at the peak activity of drugs permitted to establish the necessary dose of anticholinesterase drugs, especially after the operation.", "contents": "[Our modification of ergograph for studying muscle fatigability in myasthenia]. The authors designed and made an ergograph of own modification and elaborated normal values of fatiguability half-time on the basis of 51 healthy subjects comparing the results with those in a group of 41 myasthenics subjected to thymectomy. The fatiguability half-time was determined in groups of muscles in the hand, axilla and popliteal area and masseters before and 14 days after thymectomy. The ergographic record was obtained without application of drugs as well as at the peak of activity of drugs and at the peak of activity of those after 5 mg Tensilon dose. The investigation made it possible to determine the muscle power before and after tymectomy. The Tensioln test at the peak activity of drugs permitted to establish the necessary dose of anticholinesterase drugs, especially after the operation.", "PMID": 958575}
{"id": "pubmed23n0032_3132", "title": "[Improving radiographic diagnosis of the sella by means of direct magnification technics and autotomography].", "content": "On the basis of personal experiences with 30 cases and a survey of literature the authors discuss the value of radiological diagnostic assessment of sella using a technique of direct magnification and autotomography. The superiority of these techniques over the information obtained from plain films of the skull has been demonstrated. These techniques are easy, not time-consuming, they do not require complex apparatus and their costs approximate those of plain radiograms.", "contents": "[Improving radiographic diagnosis of the sella by means of direct magnification technics and autotomography]. On the basis of personal experiences with 30 cases and a survey of literature the authors discuss the value of radiological diagnostic assessment of sella using a technique of direct magnification and autotomography. The superiority of these techniques over the information obtained from plain films of the skull has been demonstrated. These techniques are easy, not time-consuming, they do not require complex apparatus and their costs approximate those of plain radiograms.", "PMID": 958576}
{"id": "pubmed23n0032_3133", "title": "[As yet unknown sign in internuclear ophthalmoplegia: unilateral eyelid retraction occuring during abduction or adduction of the  eye bulb].", "content": "The author presents 2 cases of internuclear ophthalmoplegia with unilateral upper lid retraction and widening of the palpebral fissure of the adducted eye with a simultaneous narrowing of the palpebral fissure of the other eye. In the 3rd case of internuclear ophthalmoplegia with paralysis of the lateral rectus muscle, the sign of upper lid retraction was present in the adduced eye.", "contents": "[As yet unknown sign in internuclear ophthalmoplegia: unilateral eyelid retraction occuring during abduction or adduction of the  eye bulb]. The author presents 2 cases of internuclear ophthalmoplegia with unilateral upper lid retraction and widening of the palpebral fissure of the adducted eye with a simultaneous narrowing of the palpebral fissure of the other eye. In the 3rd case of internuclear ophthalmoplegia with paralysis of the lateral rectus muscle, the sign of upper lid retraction was present in the adduced eye.", "PMID": 958577}
{"id": "pubmed23n0032_3134", "title": "[Analysis of clinical and social features in a group of epileptics with frequent seizures].", "content": "A group of 50 epileptics (group C) with frequent seizures refractory to treatment was studied. The control group (R) comprised 50 cases with rare seizures. It was found that despite systematic treatment (with full therapeutic doses) and frequent changes of drugs the frequency of seizures was not reduced in group C. The analysis of clinical features of both groups showed that group C as compared with group R had more patients with temporal lobe seizures, neurological abnormalities, low intelligence quotient, changed background activity in EEG and longer duration of the disease. These data may suggest a greater number of cases with detectable organic brain lesions among patients with frequent seizures.", "contents": "[Analysis of clinical and social features in a group of epileptics with frequent seizures]. A group of 50 epileptics (group C) with frequent seizures refractory to treatment was studied. The control group (R) comprised 50 cases with rare seizures. It was found that despite systematic treatment (with full therapeutic doses) and frequent changes of drugs the frequency of seizures was not reduced in group C. The analysis of clinical features of both groups showed that group C as compared with group R had more patients with temporal lobe seizures, neurological abnormalities, low intelligence quotient, changed background activity in EEG and longer duration of the disease. These data may suggest a greater number of cases with detectable organic brain lesions among patients with frequent seizures.", "PMID": 958580}
{"id": "pubmed23n0032_3135", "title": "[Evaluation of selected parameters of calcium metabolism in epileptics on long-term treatment with diphenylhydantoin].", "content": "In 11 epileptics treated with DPH for 10-20 years a significant fall was observed in the serum levels of calcium, phosphates, and ionic index, with a significant rise in the serum magnesium concentration. No detectable changes were observed in the radiological appearance of bones and alkaline phosphates activity was within normal range. In two cases histological examinations demonstrated signs of osteomalacia. The authors postulate the necessity of monitoring selected parameters of calcium metabolism in patients on long-term treatment with diphenylhydantoin.", "contents": "[Evaluation of selected parameters of calcium metabolism in epileptics on long-term treatment with diphenylhydantoin]. In 11 epileptics treated with DPH for 10-20 years a significant fall was observed in the serum levels of calcium, phosphates, and ionic index, with a significant rise in the serum magnesium concentration. No detectable changes were observed in the radiological appearance of bones and alkaline phosphates activity was within normal range. In two cases histological examinations demonstrated signs of osteomalacia. The authors postulate the necessity of monitoring selected parameters of calcium metabolism in patients on long-term treatment with diphenylhydantoin.", "PMID": 958581}
{"id": "pubmed23n0032_3136", "title": "[Clinico-morphological analysis of clinically unrecognized subdural hematomas].", "content": "The authors report clinical and morphological analysis of 31 autopsied cases of subdural haematomas unrecognized during life. The most frequent erroneous clinical diagnosis was cerebrovascular brain disease, psychiatric syndrome or brain tumour. The greatest diagnostic difficulties were encountered in cases of subdural haematoma developing in elderly subjects (in this material 24 patients were aged above 60 years). Brain atrophy delayed in them the appearance of intracranial raised pressure syndrome, coexistence of vascular changes masked the signs of subdural haematoma and frequent memory disturbances made history taking difficult. Brain atrophy in elderly subjects may be also a factor contributing to development of subdural haematoma. Widening of subdural space causes greater tension of bridging veins and their easier rupture when the brain is displaced during trauma. Absence of inflammatory changes in the neuropathological findings in cases of subdural haemorrhage suggests that the term \"chronic subdural haematoma\" is better than the older term \"pachymeningitis haemorrhagic interna\".", "contents": "[Clinico-morphological analysis of clinically unrecognized subdural hematomas]. The authors report clinical and morphological analysis of 31 autopsied cases of subdural haematomas unrecognized during life. The most frequent erroneous clinical diagnosis was cerebrovascular brain disease, psychiatric syndrome or brain tumour. The greatest diagnostic difficulties were encountered in cases of subdural haematoma developing in elderly subjects (in this material 24 patients were aged above 60 years). Brain atrophy delayed in them the appearance of intracranial raised pressure syndrome, coexistence of vascular changes masked the signs of subdural haematoma and frequent memory disturbances made history taking difficult. Brain atrophy in elderly subjects may be also a factor contributing to development of subdural haematoma. Widening of subdural space causes greater tension of bridging veins and their easier rupture when the brain is displaced during trauma. Absence of inflammatory changes in the neuropathological findings in cases of subdural haemorrhage suggests that the term \"chronic subdural haematoma\" is better than the older term \"pachymeningitis haemorrhagic interna\".", "PMID": 958579}
{"id": "pubmed23n0032_3137", "title": "[Preliminary evaluation of Rivotril in epilepsy].", "content": "Treatment with Rivotril in doses of 2-10 mg daily was given to 26 patients with various forms of epilepsy, mostly refractory to previous treatment. In 2 cases the drug was withdrawn on account of intolerance, in 4 cases treatment was stopped after several days in view of greatly increased frequency of seizures. In the remaining cases the drug was administered during 2 to 27 months, (mean 7 months) in 3 cases as the only medication and in 17 with other anticonvulsants. The best therapeutic results were obtained in patients with partial seizures of complex symptomatology and in generalized non-convulsive seizures, the worst results in generalized seizures. Electroencephalographic findings included particularly disappearance of seizure activity, while focal changes persisted and even grew worse in some cases. Apart from 2 cases of acute intolerance in another 4 cases side effects were observed with somnolence, dizziness, equilibrium disturbances, and in one case granulocytopenia developed. Allergic changes and liver or renal damage were never observed. The authors suggest introduction of the drug in treatment of epilepsy in view of its favourable clinical effect even in cases refractory to previous treatment, especially since the drug is relatively well tolerated.", "contents": "[Preliminary evaluation of Rivotril in epilepsy]. Treatment with Rivotril in doses of 2-10 mg daily was given to 26 patients with various forms of epilepsy, mostly refractory to previous treatment. In 2 cases the drug was withdrawn on account of intolerance, in 4 cases treatment was stopped after several days in view of greatly increased frequency of seizures. In the remaining cases the drug was administered during 2 to 27 months, (mean 7 months) in 3 cases as the only medication and in 17 with other anticonvulsants. The best therapeutic results were obtained in patients with partial seizures of complex symptomatology and in generalized non-convulsive seizures, the worst results in generalized seizures. Electroencephalographic findings included particularly disappearance of seizure activity, while focal changes persisted and even grew worse in some cases. Apart from 2 cases of acute intolerance in another 4 cases side effects were observed with somnolence, dizziness, equilibrium disturbances, and in one case granulocytopenia developed. Allergic changes and liver or renal damage were never observed. The authors suggest introduction of the drug in treatment of epilepsy in view of its favourable clinical effect even in cases refractory to previous treatment, especially since the drug is relatively well tolerated.", "PMID": 958582}
{"id": "pubmed23n0032_3138", "title": "[Hospital infections and their control in neurology departments].", "content": "The authors discuss briefly the problem of hospital infection, its nature, routes of spread and types of causative bacteria. A particular emphasis is laid on the problem of high risk neurological patients. Discussing the methods of hospital infection control in neurological departments the authors stress the importance of the room for minor diagnostic and therapeutic procedures since a real danger to the neurological patients is the possibility of infection during lumbar tap.", "contents": "[Hospital infections and their control in neurology departments]. The authors discuss briefly the problem of hospital infection, its nature, routes of spread and types of causative bacteria. A particular emphasis is laid on the problem of high risk neurological patients. Discussing the methods of hospital infection control in neurological departments the authors stress the importance of the room for minor diagnostic and therapeutic procedures since a real danger to the neurological patients is the possibility of infection during lumbar tap.", "PMID": 958583}
{"id": "pubmed23n0032_3139", "title": "[Effect of deep hyperventilation on lactate concentration in cerebrospinal fluid and brain tissue].", "content": "Deep hyperventilation (HV)--pCO2 less than 15 mm Hg--was applied in rabbits with normal intracranial pressure (ICP) and in cats with experimental brain oedema developed after epidural balloon compression. In animals with normal ICP there was an increase of CSF and tissue lactate concentration as soon as after 45 min. of HV. The lactate concentration was increasing all the time during HV. The resumption of normoventilation (2 h) after 10 h of HV was sufficient to bring the lactate level down near normality. Electron microscope studies showed only slight ultrastructural changes without destruction, even after 10 h HV. In animals with increased ICP, brain oedema was the main factor raising the lactate level, while the effect of HV was negligible.", "contents": "[Effect of deep hyperventilation on lactate concentration in cerebrospinal fluid and brain tissue]. Deep hyperventilation (HV)--pCO2 less than 15 mm Hg--was applied in rabbits with normal intracranial pressure (ICP) and in cats with experimental brain oedema developed after epidural balloon compression. In animals with normal ICP there was an increase of CSF and tissue lactate concentration as soon as after 45 min. of HV. The lactate concentration was increasing all the time during HV. The resumption of normoventilation (2 h) after 10 h of HV was sufficient to bring the lactate level down near normality. Electron microscope studies showed only slight ultrastructural changes without destruction, even after 10 h HV. In animals with increased ICP, brain oedema was the main factor raising the lactate level, while the effect of HV was negligible.", "PMID": 958584}
{"id": "pubmed23n0032_3140", "title": "[Hyperventilation and mannitol administration during surgery in patients with space-occupying intracranial lesions].", "content": "The aim of this work was to evaluate the effect of hyperventilation and mannitol on brain volume during neurosurgical operations. The material comprises 30 cases of supratentorial tumours. pO2, pCO2, pH and lactate concentration were determined in the arterial blood and in 7 cases also in the CSF. It was established that hyperventilation sometimes fails to decrease ICP; it was observed that hyperventilation was more effective in decreasing brain volume of the pCO2 level decreased by 14,6 mm Hg on the average. The joint use of hyperventilation and hypertonic mannitol was found to be more effective. Neither of the above methods was effective in the case of cystic tumors.", "contents": "[Hyperventilation and mannitol administration during surgery in patients with space-occupying intracranial lesions]. The aim of this work was to evaluate the effect of hyperventilation and mannitol on brain volume during neurosurgical operations. The material comprises 30 cases of supratentorial tumours. pO2, pCO2, pH and lactate concentration were determined in the arterial blood and in 7 cases also in the CSF. It was established that hyperventilation sometimes fails to decrease ICP; it was observed that hyperventilation was more effective in decreasing brain volume of the pCO2 level decreased by 14,6 mm Hg on the average. The joint use of hyperventilation and hypertonic mannitol was found to be more effective. Neither of the above methods was effective in the case of cystic tumors.", "PMID": 958585}
{"id": "pubmed23n0032_3141", "title": "The possible role of histamine in the control of prolactin and gonadotropin release.", "content": "To evaluate a possible role of histamine in the CNS control of prolactin and gonadotropin release, adult ovariectomized rats, with stainless steel cannulae implanted in the 3rd ventricle, were given s.c. injections of 10 mug of estradiol benzoate. 48 h later, 2 mul of 0.9% NaCl alone or of saline containing 1, 5, 25 or 125 mug of histamine dihydrochloride was microinjected into the ventricle. Immediately before and then 15, 30 and 60 min after, blood samples were withdrawn from etherized rats for radioimmunoassay (RIA) of serum prolactin, LH and FSH. In the histamine-injected rats, an increase in prolactin titers was observed and was highly significant in groups receiving the higher doses. A small yet significant release of LH, but not of FSH, was also observed. When 25 mug of histamine was injected directly into the pituitary or into the jugular vein, no elevations were observed, indicating a site of action in the brain. Restraint stress elevated serum prolactin and lowered serum LH and FSH in ovariectomized rats. These responses were blocked by the intrajugular injection of diphenhydramine (5 mg/kg). It is suggested that histamine may be involved in the hypothalamic control of prolactin release and possibly of gonadotropin release.", "contents": "The possible role of histamine in the control of prolactin and gonadotropin release. To evaluate a possible role of histamine in the CNS control of prolactin and gonadotropin release, adult ovariectomized rats, with stainless steel cannulae implanted in the 3rd ventricle, were given s.c. injections of 10 mug of estradiol benzoate. 48 h later, 2 mul of 0.9% NaCl alone or of saline containing 1, 5, 25 or 125 mug of histamine dihydrochloride was microinjected into the ventricle. Immediately before and then 15, 30 and 60 min after, blood samples were withdrawn from etherized rats for radioimmunoassay (RIA) of serum prolactin, LH and FSH. In the histamine-injected rats, an increase in prolactin titers was observed and was highly significant in groups receiving the higher doses. A small yet significant release of LH, but not of FSH, was also observed. When 25 mug of histamine was injected directly into the pituitary or into the jugular vein, no elevations were observed, indicating a site of action in the brain. Restraint stress elevated serum prolactin and lowered serum LH and FSH in ovariectomized rats. These responses were blocked by the intrajugular injection of diphenhydramine (5 mg/kg). It is suggested that histamine may be involved in the hypothalamic control of prolactin release and possibly of gonadotropin release.", "PMID": 958593}
{"id": "pubmed23n0032_3142", "title": "Differential effects of dopamine agonists and haloperidol on release of prolactin, thyroid stimulating hormone, growth hormone and luteinizing hormone in rats.", "content": "The dose-response effects of apomorphine and ET-495 (piribedil), 2 specific dopamine (DA) receptor stimulators, and haloperidol, a DA receptor blocker, were tested on the secretion of prolactin (PRL), thyroid stimulating hormone (TSH), growth hormone (GH) and luteinizing hormone (LH) in male rats. Both apomorphine and piribedil reduced serum PRL and TSH levels, stimulated GH release at low but not at high doses and either had no effect or tended to reduce serum LH levels. The minimal effective dose of apomorphine for reducing PRL by 30 min was 0.01 mg/kg; TSH inhibition was observed with a dose of 0.1-0.3 mg/kg. The inhibitory effects of apomorphine (1.0 mg/kg) on PRL and TSH levels were maximal by 15 min and diminished by 120 min; plasma GH was highest 120 min after injection. Thyroidectomy (10 days) markedLH elevated serum TSH, had no effect on serum PRL and inhibited the ability of apomorphine (0.1 or 0.3 mg/kg) to reduce TSH but not PRL levels. These observations may indicate that separate dopaminergic control mechanisms exist for TSH and PRL secretion. Administration of haloperidol elevated serum PRL, tended to lower TSH, dramatically reduced GH and had no effect on LH levels. Haloperidol pre-treatment blocked the effects of apomorphine on PRL, TSH, and GH secretion. The overall results of this study indicate that DA agonists inhibit PRL and TSH, stimulate GH but do not stimulate LH release in male rats.", "contents": "Differential effects of dopamine agonists and haloperidol on release of prolactin, thyroid stimulating hormone, growth hormone and luteinizing hormone in rats. The dose-response effects of apomorphine and ET-495 (piribedil), 2 specific dopamine (DA) receptor stimulators, and haloperidol, a DA receptor blocker, were tested on the secretion of prolactin (PRL), thyroid stimulating hormone (TSH), growth hormone (GH) and luteinizing hormone (LH) in male rats. Both apomorphine and piribedil reduced serum PRL and TSH levels, stimulated GH release at low but not at high doses and either had no effect or tended to reduce serum LH levels. The minimal effective dose of apomorphine for reducing PRL by 30 min was 0.01 mg/kg; TSH inhibition was observed with a dose of 0.1-0.3 mg/kg. The inhibitory effects of apomorphine (1.0 mg/kg) on PRL and TSH levels were maximal by 15 min and diminished by 120 min; plasma GH was highest 120 min after injection. Thyroidectomy (10 days) markedLH elevated serum TSH, had no effect on serum PRL and inhibited the ability of apomorphine (0.1 or 0.3 mg/kg) to reduce TSH but not PRL levels. These observations may indicate that separate dopaminergic control mechanisms exist for TSH and PRL secretion. Administration of haloperidol elevated serum PRL, tended to lower TSH, dramatically reduced GH and had no effect on LH levels. Haloperidol pre-treatment blocked the effects of apomorphine on PRL, TSH, and GH secretion. The overall results of this study indicate that DA agonists inhibit PRL and TSH, stimulate GH but do not stimulate LH release in male rats.", "PMID": 958594}
{"id": "pubmed23n0032_3143", "title": "[Chronic Horton's neuralgia treated successfully with indomethacin].", "content": "The author describes a case in which different types of headaches occurred in life. In childhood the patient had typical attacks of ophthalmic migraine, at the age of 45 years classical Horton's neuralgia developed lasting 5 months, occurring 3-4 times daily and 2-3 times in night time. The pains were intense, their location was always identical and their duration was 15-20 min. It seemed at the time of observation that they represented a form intermediate between that described by Ekbom and Olivarius and that reported by Sjaastad and Dale. After treatment with Indomethacin immediate improvement was obtained with disappearance of pains.", "contents": "[Chronic Horton's neuralgia treated successfully with indomethacin]. The author describes a case in which different types of headaches occurred in life. In childhood the patient had typical attacks of ophthalmic migraine, at the age of 45 years classical Horton's neuralgia developed lasting 5 months, occurring 3-4 times daily and 2-3 times in night time. The pains were intense, their location was always identical and their duration was 15-20 min. It seemed at the time of observation that they represented a form intermediate between that described by Ekbom and Olivarius and that reported by Sjaastad and Dale. After treatment with Indomethacin immediate improvement was obtained with disappearance of pains.", "PMID": 958589}
{"id": "pubmed23n0032_3144", "title": "[Surgical treatment of hypophyseal adenomas using the nose and sphenoid sinus approach].", "content": "A method of hypophyseal adenoma removal through the nose and sphenoidal sinus is described. The method was used in 26 patients and no postoperative complications were observed. The approach to the sella from the sphenoidal sinus makes it possible to remove the tumour from the sphenoidal sinus and from the area below and behind optic chiasm.", "contents": "[Surgical treatment of hypophyseal adenomas using the nose and sphenoid sinus approach]. A method of hypophyseal adenoma removal through the nose and sphenoidal sinus is described. The method was used in 26 patients and no postoperative complications were observed. The approach to the sella from the sphenoidal sinus makes it possible to remove the tumour from the sphenoidal sinus and from the area below and behind optic chiasm.", "PMID": 958586}
{"id": "pubmed23n0032_3145", "title": "Accumulation of 3H-testosterone in nuclear and cytoplasmic fractions of rat brain during postnatal development.", "content": "The accumulation of 3H-testosterone and its metabolites in the brain of neonatal and prepubertal rats was measured following injections of the hormone in vivo, or after incubation of minced brain tissue with 3H-testosterone in tissue culture medium. Radioactivity associated with purified nuclei and various cytoplasmic fractions of brain tissue was determined in 2-5-day, 10-12-day, and 25-32-day-old animals. Comparisons of radioactive steroids, accumulated 2 h after s.c. injections of 3H-testosterone or 3H-estradiol, showed that testosterone (T) is accumulated to a lesser extent than estradiol (E), but the age-related patterns of uptake are similar for both steroids. A continuous decrease in radioactivity was observed in nuclear and cytoplasmic fractions from the brains of 2-, 4-, and 12-day-old animals that were given injections of 3H-testosterone in vivo. This age-related pattern of decreasing uptake of radioactivity was not observed when excised brain tissue was incubated with 3H-testosterone in tissue culture medium. Thus, it appears that the age-related responsiveness of neonatal rats to T may be due more to the fact that decreasing amounts of the hormone reach the brain in older animals than that an inherent difference exists in tissue susceptibility during this period. Most of the radioactivity accumulated in the brain was associated with cytoplasmic fractions. Less than 1% of the homogenate radioactivity accumulated in purified nuclei. This restricted uptake and the kinetics of 3H-testosterone nuclear accumulation suggest that a nuclear site of action of T and/or its metabolites exists in the neonatal rat brain.", "contents": "Accumulation of 3H-testosterone in nuclear and cytoplasmic fractions of rat brain during postnatal development. The accumulation of 3H-testosterone and its metabolites in the brain of neonatal and prepubertal rats was measured following injections of the hormone in vivo, or after incubation of minced brain tissue with 3H-testosterone in tissue culture medium. Radioactivity associated with purified nuclei and various cytoplasmic fractions of brain tissue was determined in 2-5-day, 10-12-day, and 25-32-day-old animals. Comparisons of radioactive steroids, accumulated 2 h after s.c. injections of 3H-testosterone or 3H-estradiol, showed that testosterone (T) is accumulated to a lesser extent than estradiol (E), but the age-related patterns of uptake are similar for both steroids. A continuous decrease in radioactivity was observed in nuclear and cytoplasmic fractions from the brains of 2-, 4-, and 12-day-old animals that were given injections of 3H-testosterone in vivo. This age-related pattern of decreasing uptake of radioactivity was not observed when excised brain tissue was incubated with 3H-testosterone in tissue culture medium. Thus, it appears that the age-related responsiveness of neonatal rats to T may be due more to the fact that decreasing amounts of the hormone reach the brain in older animals than that an inherent difference exists in tissue susceptibility during this period. Most of the radioactivity accumulated in the brain was associated with cytoplasmic fractions. Less than 1% of the homogenate radioactivity accumulated in purified nuclei. This restricted uptake and the kinetics of 3H-testosterone nuclear accumulation suggest that a nuclear site of action of T and/or its metabolites exists in the neonatal rat brain.", "PMID": 958595}
{"id": "pubmed23n0032_3146", "title": "The effect of surgical isolation or ablation of the medial basal hypothalamus on serum prolactin levels in male rats.", "content": "Complete isolation of ablation of the medial basal hypothalamus (MBH) was performed in adult male rats, and non-stress prolactin levels were measured by radioimmunoassay in blood collected by decapitation at 24h, 2 weeks and 6 weeks following surgery. 24 h after surgery, medial hypothalamic ablation (MHA) markedly elevated prolactin levels compared to those of intact control rats; prolactin levels subsequently declined to normal resting values 2 to 6 weeks following surgery. Circulating prolactin levels following hypothalamic isolation were not different from those of controls at either acute or chronic sampling intervals. These data suggest that chronic isolation or destruction of the MBH does not alter basal prolactin secretion in male rats, provided that circadian and stress-induced variations in control animals are properly considered.", "contents": "The effect of surgical isolation or ablation of the medial basal hypothalamus on serum prolactin levels in male rats. Complete isolation of ablation of the medial basal hypothalamus (MBH) was performed in adult male rats, and non-stress prolactin levels were measured by radioimmunoassay in blood collected by decapitation at 24h, 2 weeks and 6 weeks following surgery. 24 h after surgery, medial hypothalamic ablation (MHA) markedly elevated prolactin levels compared to those of intact control rats; prolactin levels subsequently declined to normal resting values 2 to 6 weeks following surgery. Circulating prolactin levels following hypothalamic isolation were not different from those of controls at either acute or chronic sampling intervals. These data suggest that chronic isolation or destruction of the MBH does not alter basal prolactin secretion in male rats, provided that circadian and stress-induced variations in control animals are properly considered.", "PMID": 958596}
{"id": "pubmed23n0032_3147", "title": "[Progressive spinal muscular atrophy of proximal location in adults].", "content": "The authors report two cases of familial progressive muscular atrophy of proximal localization, with late onset of signs which was associated with involvement of the motor nucleus of trigeminal nerve and hypoglossus nerve nucleus as well as with presence of gynaecomastia. Cases of coexistence of gynaecomastia with spinal muscular atrophy are rare in the literature and the present report may serve for explanation whether the association of these signs is accidental or whether it is a separate nosological entity.", "contents": "[Progressive spinal muscular atrophy of proximal location in adults]. The authors report two cases of familial progressive muscular atrophy of proximal localization, with late onset of signs which was associated with involvement of the motor nucleus of trigeminal nerve and hypoglossus nerve nucleus as well as with presence of gynaecomastia. Cases of coexistence of gynaecomastia with spinal muscular atrophy are rare in the literature and the present report may serve for explanation whether the association of these signs is accidental or whether it is a separate nosological entity.", "PMID": 958590}
{"id": "pubmed23n0032_3148", "title": "Effects of ovariectomy on dexamethasone suppression of the adrenal axis in adult rats.", "content": "Three weeks after ovariectomy, adult female rats had lower basal levels of serum corticosterone (B), which did not display a daily rhythm, and reduced responses to ether stress, measured by blood levels of B 15 min after ether exposure. Ovariectomized (OVX) females were more sensitive to dexamethasone (DEX) suppression of the ether induced stress response, reaching basal levels at a dose of 50 mug DEX/100 g b.w. At this dose, B levels were reduced by only 30% over saline-control values in intact rats. DEX-treated intact rats displayed a short-term suppression, reaching a maximum 2 h after the injection followed by a rebound 7 h post-injection and a second suppression period evident by 11 h post-injection. OVX rats showed a steadily increasing suppression that began 1 h after injection and persisted to the last sample time at 11 h post-injection. The disappearance of DEX from peripheral blood was followed by means of radioimmunoassay and no difference was found between intact and OVX rats either in the basal state or 15 min after ether stress. It can be concluded that ovarian steroids condition the sensitivity of the adrenal axis to DEX suppression and that the differences in DEX sensitivity  we have previously noted between prepubertal and adult rats can be accounted for by a change in gonadal status rather than by a critical developmental event in the adrenal axis itself.", "contents": "Effects of ovariectomy on dexamethasone suppression of the adrenal axis in adult rats. Three weeks after ovariectomy, adult female rats had lower basal levels of serum corticosterone (B), which did not display a daily rhythm, and reduced responses to ether stress, measured by blood levels of B 15 min after ether exposure. Ovariectomized (OVX) females were more sensitive to dexamethasone (DEX) suppression of the ether induced stress response, reaching basal levels at a dose of 50 mug DEX/100 g b.w. At this dose, B levels were reduced by only 30% over saline-control values in intact rats. DEX-treated intact rats displayed a short-term suppression, reaching a maximum 2 h after the injection followed by a rebound 7 h post-injection and a second suppression period evident by 11 h post-injection. OVX rats showed a steadily increasing suppression that began 1 h after injection and persisted to the last sample time at 11 h post-injection. The disappearance of DEX from peripheral blood was followed by means of radioimmunoassay and no difference was found between intact and OVX rats either in the basal state or 15 min after ether stress. It can be concluded that ovarian steroids condition the sensitivity of the adrenal axis to DEX suppression and that the differences in DEX sensitivity  we have previously noted between prepubertal and adult rats can be accounted for by a change in gonadal status rather than by a critical developmental event in the adrenal axis itself.", "PMID": 958597}
{"id": "pubmed23n0032_3149", "title": "[Development of papilledema in cases of tumors of the lower segment of the vertebral canal].", "content": "The author discusses the problem of the mechanism of intracranial hypertension developing with papilloedema in cases of tumours situated in the lower parts of vertebral canal. The author discusses the present data in literature and an own case in which manifestations of intracranial hypertension with papilloedema developed in a 48-year-old man with neuroma situated in the vertebral canal at the level of 11th thoracic vertebra. In a discussion on the problem the author stresse that his case failed to confirm the accepted view that the cause of intracranial hypertension in cases of tumours in the lower part of the vertebral canal should be sought in increased protein level in the cerebrospinal fluid. Attention was called also to the fact that in most cases described as yet presence of gliomas was demonstrated and the cerebrospinal fluid pressure was never so high (700 mm H2O in lying position).", "contents": "[Development of papilledema in cases of tumors of the lower segment of the vertebral canal]. The author discusses the problem of the mechanism of intracranial hypertension developing with papilloedema in cases of tumours situated in the lower parts of vertebral canal. The author discusses the present data in literature and an own case in which manifestations of intracranial hypertension with papilloedema developed in a 48-year-old man with neuroma situated in the vertebral canal at the level of 11th thoracic vertebra. In a discussion on the problem the author stresse that his case failed to confirm the accepted view that the cause of intracranial hypertension in cases of tumours in the lower part of the vertebral canal should be sought in increased protein level in the cerebrospinal fluid. Attention was called also to the fact that in most cases described as yet presence of gliomas was demonstrated and the cerebrospinal fluid pressure was never so high (700 mm H2O in lying position).", "PMID": 958592}
{"id": "pubmed23n0032_3150", "title": "Responses of plasma \"estradiol\" and plasma LH to ovariectomy, ovariectomy plus adrenalectomy, and estrogen injection at various ages.", "content": "Plasma LH and \"estradiol\" were measured by radioimmunoassay in female rats 5, 10, 15, 20, 25, 30, 40, and 80 days of age, and the changes in these hormone levels 24 h after ovariectomy, ovariectomy plus adrenalectomy, and estrogen injection were determined. Plasma \"estradiol\" was high at 5 days of age; it increased further to a peak at 15 days of age, and declined thereafter to the low levels seen in adult rats. At each age, plasma \"estradiol\" declined about 50% 24 h after ovariectomy, whereas it fell to undetectable levels 24 h after ovariectomy plus adrenalectomy at 20 and 80 days of age. Plasma LH did not increase 24 h following ovariectomy at 5 days of age, but it did at all ages thereafter. The peak increase occurred 24 h after ovariectomy at 15 days of age, and the increment was progressively smaller at older ages. Ovariectomy plus adrenalectomy did not cause a significantly greater increase in plasma LH than ovariectomy alone. Injection of estradiol caused a decrease in plasms LH at all ages except in the animals ovariectomized at 5 days of age. The greatest decrease was seen in animals ovariectomized at 15 days of age, with a progressively smaller negative feedback response as age increased. In addition to providing systematic data on changes in the negative feedback effect of estradiol at various ages, the results indicate that, especially in immature animals, there is a circulating substance which cross-reacts with antibodies to estradiol but which does not appear to be a biologically-active estrogen. It does not come from the ovaries and disappears from the circulation if the adrenals as well as the ovaries are removed.", "contents": "Responses of plasma \"estradiol\" and plasma LH to ovariectomy, ovariectomy plus adrenalectomy, and estrogen injection at various ages. Plasma LH and \"estradiol\" were measured by radioimmunoassay in female rats 5, 10, 15, 20, 25, 30, 40, and 80 days of age, and the changes in these hormone levels 24 h after ovariectomy, ovariectomy plus adrenalectomy, and estrogen injection were determined. Plasma \"estradiol\" was high at 5 days of age; it increased further to a peak at 15 days of age, and declined thereafter to the low levels seen in adult rats. At each age, plasma \"estradiol\" declined about 50% 24 h after ovariectomy, whereas it fell to undetectable levels 24 h after ovariectomy plus adrenalectomy at 20 and 80 days of age. Plasma LH did not increase 24 h following ovariectomy at 5 days of age, but it did at all ages thereafter. The peak increase occurred 24 h after ovariectomy at 15 days of age, and the increment was progressively smaller at older ages. Ovariectomy plus adrenalectomy did not cause a significantly greater increase in plasma LH than ovariectomy alone. Injection of estradiol caused a decrease in plasms LH at all ages except in the animals ovariectomized at 5 days of age. The greatest decrease was seen in animals ovariectomized at 15 days of age, with a progressively smaller negative feedback response as age increased. In addition to providing systematic data on changes in the negative feedback effect of estradiol at various ages, the results indicate that, especially in immature animals, there is a circulating substance which cross-reacts with antibodies to estradiol but which does not appear to be a biologically-active estrogen. It does not come from the ovaries and disappears from the circulation if the adrenals as well as the ovaries are removed.", "PMID": 958598}
{"id": "pubmed23n0032_3151", "title": "[Traumatic injury of ciliary ganglion].", "content": "The authors report a case in which slight cranial trauma was followed by manifestations of damage to the right ciliary ganglion. After 3 weeks the signs of damage began to regress and disappeared completely. Laboratory investigations failed to demonstrate any abnormalities. Apart from this trauma there were no other detectable causes, and especially there were no history data suggesting past infection, botulism or syphilis. The mechanism of ciliary ganglion injury is not known (contusion, haematoma, oedema etc.).", "contents": "[Traumatic injury of ciliary ganglion]. The authors report a case in which slight cranial trauma was followed by manifestations of damage to the right ciliary ganglion. After 3 weeks the signs of damage began to regress and disappeared completely. Laboratory investigations failed to demonstrate any abnormalities. Apart from this trauma there were no other detectable causes, and especially there were no history data suggesting past infection, botulism or syphilis. The mechanism of ciliary ganglion injury is not known (contusion, haematoma, oedema etc.).", "PMID": 958588}
{"id": "pubmed23n0032_3152", "title": "[Malignant meningioma with extracranial metastases].", "content": "The authors report a case of primary malignant meningioma of meninges in the right frontal area with metastases to meninges in the right temporal area and left atrial endocardium and with emboli composed of malignant cells in the vessels of the myocardium, spleen, liver, lungs and pancreas in a 49-year-old woman after commissurotomy performed for rheumatic valvular heart disease.", "contents": "[Malignant meningioma with extracranial metastases]. The authors report a case of primary malignant meningioma of meninges in the right frontal area with metastases to meninges in the right temporal area and left atrial endocardium and with emboli composed of malignant cells in the vessels of the myocardium, spleen, liver, lungs and pancreas in a 49-year-old woman after commissurotomy performed for rheumatic valvular heart disease.", "PMID": 958591}
{"id": "pubmed23n0032_3153", "title": "The megadolichobasilar artery anomaly and expansion of the internal auditory meatus.", "content": "A case of megadolichobasilar artery anomaly (MDBA) with expansion of the internal auditory meatus is presented. There was no associated hearing loss and it is postulated that IAM expansion without hearing loss may occur with this anomaly.", "contents": "The megadolichobasilar artery anomaly and expansion of the internal auditory meatus. A case of megadolichobasilar artery anomaly (MDBA) with expansion of the internal auditory meatus is presented. There was no associated hearing loss and it is postulated that IAM expansion without hearing loss may occur with this anomaly.", "PMID": 958610}
{"id": "pubmed23n0032_3154", "title": "Rare carotid-vertebrobasilar anastomoses with notes on the differentiation between proatlantal and hypoglossal arteries.", "content": "Two cases of persistent anastomoses between the carotid and vertebrobasilar systems which have only once been previously reported are described. These are a proatlantal intersegmental artery which arises from the external carotid artery and a primitive trigeminal artery with direct anastomosis to the superior cerebellar artery. Angiographic criteria for differentiation of the proatlantal and more common hypoglossal arteries, as well as the rare persistent cervical intersegmental artery are also presented.", "contents": "Rare carotid-vertebrobasilar anastomoses with notes on the differentiation between proatlantal and hypoglossal arteries. Two cases of persistent anastomoses between the carotid and vertebrobasilar systems which have only once been previously reported are described. These are a proatlantal intersegmental artery which arises from the external carotid artery and a primitive trigeminal artery with direct anastomosis to the superior cerebellar artery. Angiographic criteria for differentiation of the proatlantal and more common hypoglossal arteries, as well as the rare persistent cervical intersegmental artery are also presented.", "PMID": 958611}
{"id": "pubmed23n0032_3155", "title": "The radiological manifestations of intracranial rhabdomyosarcoma.", "content": "Two cases of rhabdomyosarcoma, one arising in the parotid gland and the other within the middle ear cavity, are presented. Both cases developed neurological signs of intracranial extension, which, in one, were non-localizing. The angiographic characteristics of these two cases as well as those reported in the literature are discussed. Angiography delineated the extent of tumor involvement and influenced the treatment ports. Isolated reports of long term survivals and some promising preliminary results from more aggressive therapy warrant delineation of tumor extent. To this end angiography is helpful.", "contents": "The radiological manifestations of intracranial rhabdomyosarcoma. Two cases of rhabdomyosarcoma, one arising in the parotid gland and the other within the middle ear cavity, are presented. Both cases developed neurological signs of intracranial extension, which, in one, were non-localizing. The angiographic characteristics of these two cases as well as those reported in the literature are discussed. Angiography delineated the extent of tumor involvement and influenced the treatment ports. Isolated reports of long term survivals and some promising preliminary results from more aggressive therapy warrant delineation of tumor extent. To this end angiography is helpful.", "PMID": 958612}
{"id": "pubmed23n0032_3156", "title": "Aspergillosis involving the sphenoid sinus.", "content": "The radiological features of isolated involvement of the sphenoid sinus by aspergillosis are described.", "contents": "Aspergillosis involving the sphenoid sinus. The radiological features of isolated involvement of the sphenoid sinus by aspergillosis are described.", "PMID": 958613}
{"id": "pubmed23n0032_3157", "title": "Suprasellar subarachnoid cysts.", "content": "Suprasellar subarachnoid cysts are rare entities that should be included in the differential diagnosis of parasellar masses. Five new cases are reported and the literature reviewed.", "contents": "Suprasellar subarachnoid cysts. Suprasellar subarachnoid cysts are rare entities that should be included in the differential diagnosis of parasellar masses. Five new cases are reported and the literature reviewed.", "PMID": 958614}
{"id": "pubmed23n0032_3158", "title": "The plica mediana dorsalis of the dura mater and its relation to lumbar peridurography (canalography).", "content": "Lewit and Sereghy (1975) have expressed some doubts about the existence of the dorsal plica mediana and its relation to the median translucency regularly seen on peridurographic pictures made in the PA projection. The present paper reports the demonstration of the dorsal plica mediana in fresh anatomical preparations under conditions lowering the intradural pressure, and the relation of the plica to the peridurographical median translucency, shown on the basis of the injection into the lumbar peridural space of a gelatine solution containing India ink.", "contents": "The plica mediana dorsalis of the dura mater and its relation to lumbar peridurography (canalography). Lewit and Sereghy (1975) have expressed some doubts about the existence of the dorsal plica mediana and its relation to the median translucency regularly seen on peridurographic pictures made in the PA projection. The present paper reports the demonstration of the dorsal plica mediana in fresh anatomical preparations under conditions lowering the intradural pressure, and the relation of the plica to the peridurographical median translucency, shown on the basis of the injection into the lumbar peridural space of a gelatine solution containing India ink.", "PMID": 958615}
{"id": "pubmed23n0032_3159", "title": "The venous drainage of the tectum mesencephali. An anatomical angiographic study of the quadrigeminal veins.", "content": "The normal course and appearance of the veins draining the tectum mesencephali proper are described in detail on the basis of the anatomical radiological study. These specific veins, the quadrigeminal veins, were constantly present on autopsy study. There are two types of variations of these quadrigeminal veins. They have a close anatomical and angiographic correlation with the quadrigeminal plate proper, and also outline the posterior aspects of the quadrigeminal plate. The whole structure of the mesencephalon may be delineated by these veins in combination with the other veins of the mesencephalon. They may be of diagnostic value in the early recognition of the brain stem lesions.", "contents": "The venous drainage of the tectum mesencephali. An anatomical angiographic study of the quadrigeminal veins. The normal course and appearance of the veins draining the tectum mesencephali proper are described in detail on the basis of the anatomical radiological study. These specific veins, the quadrigeminal veins, were constantly present on autopsy study. There are two types of variations of these quadrigeminal veins. They have a close anatomical and angiographic correlation with the quadrigeminal plate proper, and also outline the posterior aspects of the quadrigeminal plate. The whole structure of the mesencephalon may be delineated by these veins in combination with the other veins of the mesencephalon. They may be of diagnostic value in the early recognition of the brain stem lesions.", "PMID": 958616}
{"id": "pubmed23n0032_3160", "title": "Myelographic evidence for nerve root enlargement in a case of Charcot-Marie-Tooth disease.", "content": "The myelographic findings of enlarged nerve roots in a patient with Charcot-Marie-Tooth disease is found to be identical to those of Hypertrophic Interstitial Neuritis and Neurofibromatosis. The hypertrophy of the roots may represent a single response to different noxious stimuli. Clinical and laboratory differentiation are given for the diseases.", "contents": "Myelographic evidence for nerve root enlargement in a case of Charcot-Marie-Tooth disease. The myelographic findings of enlarged nerve roots in a patient with Charcot-Marie-Tooth disease is found to be identical to those of Hypertrophic Interstitial Neuritis and Neurofibromatosis. The hypertrophy of the roots may represent a single response to different noxious stimuli. Clinical and laboratory differentiation are given for the diseases.", "PMID": 958617}
{"id": "pubmed23n0032_3161", "title": "Focal angiographic abnormalities with subdural empyema.", "content": "Thirteen patients with subdural empyema underwent arteriography preoperatively and three were restudied postoperatively. Arterial vascular changes were found in nine cases and consisted of localized or diffuse arterial irregularities. In two cases the arterial changes resolved with effective therapy. These arterial changes are similar in appearance and response to therapy to those noted with meningitis; however, in this series they were shown to have occured in seven cases with subdural empyema but without associated meningitis. Therefore, empyema alone may cause localized or diffuse arterial irregularities.", "contents": "Focal angiographic abnormalities with subdural empyema. Thirteen patients with subdural empyema underwent arteriography preoperatively and three were restudied postoperatively. Arterial vascular changes were found in nine cases and consisted of localized or diffuse arterial irregularities. In two cases the arterial changes resolved with effective therapy. These arterial changes are similar in appearance and response to therapy to those noted with meningitis; however, in this series they were shown to have occured in seven cases with subdural empyema but without associated meningitis. Therefore, empyema alone may cause localized or diffuse arterial irregularities.", "PMID": 958618}
{"id": "pubmed23n0032_3162", "title": "Lumbar phlebography by catheterization of the lateral sacral and ascending lumbar veins with abdominal compression.", "content": "The lumbar epidural veins are opacified by injection of the lateral sacral and ascending lumbar veins with abdominal compression. This technique provides a good opacification of the entire lumbar epidural venous system. Some anatomical points are discussed and clarified. The interest of the technique in the diagnosis of discal herniations is emphasized.", "contents": "Lumbar phlebography by catheterization of the lateral sacral and ascending lumbar veins with abdominal compression. The lumbar epidural veins are opacified by injection of the lateral sacral and ascending lumbar veins with abdominal compression. This technique provides a good opacification of the entire lumbar epidural venous system. Some anatomical points are discussed and clarified. The interest of the technique in the diagnosis of discal herniations is emphasized.", "PMID": 958619}
{"id": "pubmed23n0032_3163", "title": "The economy of the EMI-scanner.", "content": "It is shown-taking in consideration the local circumstances-that the EMI scanning of the brain is less expensive than pneumoencephalography percutaneus carotid angiography or cerebral four vessel angiography.", "contents": "The economy of the EMI-scanner. It is shown-taking in consideration the local circumstances-that the EMI scanning of the brain is less expensive than pneumoencephalography percutaneus carotid angiography or cerebral four vessel angiography.", "PMID": 958620}
{"id": "pubmed23n0032_3164", "title": "Rupture of intracranial aneurysm during carotid angiography.", "content": "The reports of rupture of an aneurysm during carotid angiography are very few but the actual incidence is doubtless higher than recorded. The case presented is documented with the demonstration of a large middle cerebral artery aneurysm with an intratemporal hematoma, and, in sequence, the rupture of the aneurysm during the height of the injection and the subsequent extensive leakage of the contrast medium. The factors which can lead to rupture of an aneurysm during the angiographic procedure are discussed.", "contents": "Rupture of intracranial aneurysm during carotid angiography. The reports of rupture of an aneurysm during carotid angiography are very few but the actual incidence is doubtless higher than recorded. The case presented is documented with the demonstration of a large middle cerebral artery aneurysm with an intratemporal hematoma, and, in sequence, the rupture of the aneurysm during the height of the injection and the subsequent extensive leakage of the contrast medium. The factors which can lead to rupture of an aneurysm during the angiographic procedure are discussed.", "PMID": 958621}
{"id": "pubmed23n0032_3165", "title": "Computer tomography in the diagnosis of cerebellopontine angle tumours.", "content": "53 patients clinically suspected of having a cerebellopontine angle (CPA) lesion were examined by computer tomography (CT) with 160 X 160 matrix EMI scanner. 17 cases (32%) had tumour positive CT,  of which 12 were neurinomas and 1 meningioma. 1 CT suggestive of a CPA lesion was false positive and 1 unoperated case is probably a false negative CT. Three of the eleven verified neurinomas (27%) were of the medial type originating in the angle cistern. One neurinoma protruding 1 cm into the cistern showed no contrast enhancement. 2 CT scans (3.8%) were unsatisfactory due to movements and the large size of the head. CT is valuable for the investigation of CPA pathology and the diagnostic efficiency compares favourably to other neuroradiological procedures.", "contents": "Computer tomography in the diagnosis of cerebellopontine angle tumours. 53 patients clinically suspected of having a cerebellopontine angle (CPA) lesion were examined by computer tomography (CT) with 160 X 160 matrix EMI scanner. 17 cases (32%) had tumour positive CT,  of which 12 were neurinomas and 1 meningioma. 1 CT suggestive of a CPA lesion was false positive and 1 unoperated case is probably a false negative CT. Three of the eleven verified neurinomas (27%) were of the medial type originating in the angle cistern. One neurinoma protruding 1 cm into the cistern showed no contrast enhancement. 2 CT scans (3.8%) were unsatisfactory due to movements and the large size of the head. CT is valuable for the investigation of CPA pathology and the diagnostic efficiency compares favourably to other neuroradiological procedures.", "PMID": 958622}
{"id": "pubmed23n0032_3166", "title": "Scalp veins as collateral pathway with parasagittal meningiomas occluding the superior sagittal sinus.", "content": "In 3 cases with occlusion of the superior sagittal sinus (SSS) with parasagittal meningiomas, carotid angiography demonstrated scalp veins as an important collateral venous pathway. The authors believe that occlusion of the SSS is best seen in half axial oblique carotid phlebograms under contralateral carotid compression. Direct sinography is time consuming and troublesome. In the presence of scalp veins as important collaterals, strategic consideration for surgical treatment is discussed.", "contents": "Scalp veins as collateral pathway with parasagittal meningiomas occluding the superior sagittal sinus. In 3 cases with occlusion of the superior sagittal sinus (SSS) with parasagittal meningiomas, carotid angiography demonstrated scalp veins as an important collateral venous pathway. The authors believe that occlusion of the SSS is best seen in half axial oblique carotid phlebograms under contralateral carotid compression. Direct sinography is time consuming and troublesome. In the presence of scalp veins as important collaterals, strategic consideration for surgical treatment is discussed.", "PMID": 958623}
{"id": "pubmed23n0032_3167", "title": "Arachnoid cyst simulating intrameatal acoustic neuroma.", "content": "A very rare cause of perception deafness wass found in a child with an intrameatal arachnoid cyst. This case is the second reported in the literature.", "contents": "Arachnoid cyst simulating intrameatal acoustic neuroma. A very rare cause of perception deafness wass found in a child with an intrameatal arachnoid cyst. This case is the second reported in the literature.", "PMID": 958624}
{"id": "pubmed23n0032_3168", "title": "Unusual angiographic manifestations of craniopharyngioma: a case report.", "content": "A craniopharyngioma with two unusual manifestations, a dense capsular stain and occlusion of the internal carotid artery, is reported. Neither of these findings appear to have been reported previously. Explanations for these unusual phenomena are offered.", "contents": "Unusual angiographic manifestations of craniopharyngioma: a case report. A craniopharyngioma with two unusual manifestations, a dense capsular stain and occlusion of the internal carotid artery, is reported. Neither of these findings appear to have been reported previously. Explanations for these unusual phenomena are offered.", "PMID": 958626}
{"id": "pubmed23n0032_3169", "title": "Acquired basal encephalocele.", "content": "The radiodiagnostic signs of congenital basal encephalocele are well known. The case history described here shows that a long existing, acquired, traumatic transethmoidal encephalocele can cause the same roentgen appearance. The differences between the congenital and recent posttraumatic forms are indicated.", "contents": "Acquired basal encephalocele. The radiodiagnostic signs of congenital basal encephalocele are well known. The case history described here shows that a long existing, acquired, traumatic transethmoidal encephalocele can cause the same roentgen appearance. The differences between the congenital and recent posttraumatic forms are indicated.", "PMID": 958625}
{"id": "pubmed23n0032_3170", "title": "Tolosa-Hunt syndrome, phlebographically controled after recovery.", "content": "A 27 year old patient, presenting clinically and phlebographically a Tolosa-Hunt syndrome was observed after recovery. Normal reappearance of a previously completely obstructed cavernous sinus was noted, leaving only a persisting irregularity of the superior ophthalmic vein.", "contents": "Tolosa-Hunt syndrome, phlebographically controled after recovery. A 27 year old patient, presenting clinically and phlebographically a Tolosa-Hunt syndrome was observed after recovery. Normal reappearance of a previously completely obstructed cavernous sinus was noted, leaving only a persisting irregularity of the superior ophthalmic vein.", "PMID": 958627}
{"id": "pubmed23n0032_3171", "title": "The development of priorities for health services research. The National Center, 1974-76.", "content": "The priorities published by the  National Center for Health Services Research/DHEW in 1975 were the result of more than a year of consultation between federal officials and members of the research community. The process of priority development emerged from an interpretation of the history of the National Center, of health services research as an activity, and of the previous experience of various research agencies. This paper is a combined memoir and intellectual history of the priority development process in the National Center by a participant who, because of his position, was somewhat detached from events.", "contents": "The development of priorities for health services research. The National Center, 1974-76. The priorities published by the  National Center for Health Services Research/DHEW in 1975 were the result of more than a year of consultation between federal officials and members of the research community. The process of priority development emerged from an interpretation of the history of the National Center, of health services research as an activity, and of the previous experience of various research agencies. This paper is a combined memoir and intellectual history of the priority development process in the National Center by a participant who, because of his position, was somewhat detached from events.", "PMID": 958628}
{"id": "pubmed23n0032_3172", "title": "Child  health in America: toward a rational public policy.", "content": "Analysis of currently available data on mortality and morbidity indicates that the major organic illnesses of childhood, and their developmental consequences, are susceptible in part to the technical interventions of American medical science. Environmental forces, however, exert a powerful impact on the health of children in the United states, manifested both in the disproportionate toll of most organic diseases on poor and nonwhite populations and in such increasingly important symptoms of familial, social, and behavioral distress as child abuse, accidents, and childhood suicide. Review of the nature, quality, and distribution of child health services demonstrates a systemic inability to reach and treat the children most in need of them. A rational basis for child health policy includes: appropriate concepts of health, disease, and preventive and therapeutic intervention; a capacity to acknowledge, to measure, and to act on the familial and environmental, as well as the medical, sources of illness; an orientation to the developmental and social implications of good and poor child health; and a commitment to enable all children to receive health services. The data and this policy framework lead to these program recommendations: the channeling of resources into a more rational system which guarantees equity and access; a planning and program implementation mechanism which addresses the health needs of diverse local populations  and which makes real the  advocacy concept; a screening, evaluation, and surveillance methodology; a delivery system which both applies preventive and curative health technology and addresses basic life needs of children; and a coherent program for the training, assignment, and supervision of the several kinds of manpower which such a system would require.", "contents": "Child  health in America: toward a rational public policy. Analysis of currently available data on mortality and morbidity indicates that the major organic illnesses of childhood, and their developmental consequences, are susceptible in part to the technical interventions of American medical science. Environmental forces, however, exert a powerful impact on the health of children in the United states, manifested both in the disproportionate toll of most organic diseases on poor and nonwhite populations and in such increasingly important symptoms of familial, social, and behavioral distress as child abuse, accidents, and childhood suicide. Review of the nature, quality, and distribution of child health services demonstrates a systemic inability to reach and treat the children most in need of them. A rational basis for child health policy includes: appropriate concepts of health, disease, and preventive and therapeutic intervention; a capacity to acknowledge, to measure, and to act on the familial and environmental, as well as the medical, sources of illness; an orientation to the developmental and social implications of good and poor child health; and a commitment to enable all children to receive health services. The data and this policy framework lead to these program recommendations: the channeling of resources into a more rational system which guarantees equity and access; a planning and program implementation mechanism which addresses the health needs of diverse local populations  and which makes real the  advocacy concept; a screening, evaluation, and surveillance methodology; a delivery system which both applies preventive and curative health technology and addresses basic life needs of children; and a coherent program for the training, assignment, and supervision of the several kinds of manpower which such a system would require.", "PMID": 958629}
{"id": "pubmed23n0032_3173", "title": "A method of estimating physician requirements.", "content": "This article describes and applies a method of estimating physician requirements for the United States based on physician utilization rates of members of two comprehensive prepaid plans of medical care providing first-dollar coverage for practically all physician services. The plan members' physician utilization rates by age and sex and by field of specialty of the physician were extrapolated to the entire population of the United States. On the basis of data for 1966, it was found that 34 percent more physicians than were available would have been required to give the entire population the amount and type of care received by the plan members. The \"shortage\" of primary care physicians (general practice, internal medicine, and pediatrics combined) was found to be considerably greater than of physicians in the surgical specialties taken together (41 percent as compared to 21 percent). The paper discusses in detail the various assumptions underlying this method and stresses the need for careful evaluation of all methods of estimating physician requirements.", "contents": "A method of estimating physician requirements. This article describes and applies a method of estimating physician requirements for the United States based on physician utilization rates of members of two comprehensive prepaid plans of medical care providing first-dollar coverage for practically all physician services. The plan members' physician utilization rates by age and sex and by field of specialty of the physician were extrapolated to the entire population of the United States. On the basis of data for 1966, it was found that 34 percent more physicians than were available would have been required to give the entire population the amount and type of care received by the plan members. The \"shortage\" of primary care physicians (general practice, internal medicine, and pediatrics combined) was found to be considerably greater than of physicians in the surgical specialties taken together (41 percent as compared to 21 percent). The paper discusses in detail the various assumptions underlying this method and stresses the need for careful evaluation of all methods of estimating physician requirements.", "PMID": 958630}
{"id": "pubmed23n0032_3174", "title": "Crowding and human reproduction.", "content": "Studies of crowding in lower animals repeatedly demonstrate that compressed living conditions depress the ability of the species to reproduce. In a sample of 470 urban  Toronto women we examined the effect of neighborhood and household crowding on the probability of a pregnancy and on the probability that any given product of the pregnancy will not survive until one year of age. Crowding did not influence fertility nor fetal and infant survival within the range of crowding found in this  sample. If crowding has any influence on reproduction it would more profitably be investigated beyond the range of living density and housing markets found in North American urban areas.", "contents": "Crowding and human reproduction. Studies of crowding in lower animals repeatedly demonstrate that compressed living conditions depress the ability of the species to reproduce. In a sample of 470 urban  Toronto women we examined the effect of neighborhood and household crowding on the probability of a pregnancy and on the probability that any given product of the pregnancy will not survive until one year of age. Crowding did not influence fertility nor fetal and infant survival within the range of crowding found in this  sample. If crowding has any influence on reproduction it would more profitably be investigated beyond the range of living density and housing markets found in North American urban areas.", "PMID": 958631}
{"id": "pubmed23n0032_3175", "title": "Hypertension control among employed persons in New York City: 1973-75.", "content": "A recent survey of 8, 579 employed persons in New York City has revealed that 85.7 percent of the 1,440 hypertensives identified were already aware of their condition and 66.1 percent were being treated. These results are in marked contrast to those obtained only a few years ago, when in a similar setting only half of those with high blood pressure were aware of their condition and relatively few had entered treatment. Unfortunately, however, the potential impact of this considerable shift in behavior has  not been realized because only half of those who enter treatment achieve normal blood pressure. Thus, despite far greater emphasis on the community problem of hypertenstion, fewer than one-third of all those with high blood pressure are controlled. These data indicate that further screening and referral can, at best, increase the rate of control only slightly. The urgent need now is for development and implementation of programs that provide effective long-term patient management.", "contents": "Hypertension control among employed persons in New York City: 1973-75. A recent survey of 8, 579 employed persons in New York City has revealed that 85.7 percent of the 1,440 hypertensives identified were already aware of their condition and 66.1 percent were being treated. These results are in marked contrast to those obtained only a few years ago, when in a similar setting only half of those with high blood pressure were aware of their condition and relatively few had entered treatment. Unfortunately, however, the potential impact of this considerable shift in behavior has  not been realized because only half of those who enter treatment achieve normal blood pressure. Thus, despite far greater emphasis on the community problem of hypertenstion, fewer than one-third of all those with high blood pressure are controlled. These data indicate that further screening and referral can, at best, increase the rate of control only slightly. The urgent need now is for development and implementation of programs that provide effective long-term patient management.", "PMID": 958632}
{"id": "pubmed23n0032_3176", "title": "Head position training with the cerebral palsied child: an application of biofeedback techniques.", "content": "A biofeedback training system has been developed for providing the cerebral palsied child with precise auditory and visual information regarding the spatial position of his head. The child actively practices correcting his head position by using feedback delivered through a mercury-switch device called a head position trainer. Twelve children have received clinical training and results are discussed in terms of feedback-sensitive, feedback-trained and feedback-learned, the results of treatment dictating the classification. It is concluded that the head position trainer is an effective sensory aid for the cerebral palsied child in the development of head control and position awareness. It is proposed that this system has excellent potential use as an adjunct to regular therapy programming in that it provides additional daily practice time and an objective method of recording the child's progress.", "contents": "Head position training with the cerebral palsied child: an application of biofeedback techniques. A biofeedback training system has been developed for providing the cerebral palsied child with precise auditory and visual information regarding the spatial position of his head. The child actively practices correcting his head position by using feedback delivered through a mercury-switch device called a head position trainer. Twelve children have received clinical training and results are discussed in terms of feedback-sensitive, feedback-trained and feedback-learned, the results of treatment dictating the classification. It is concluded that the head position trainer is an effective sensory aid for the cerebral palsied child in the development of head control and position awareness. It is proposed that this system has excellent potential use as an adjunct to regular therapy programming in that it provides additional daily practice time and an objective method of recording the child's progress.", "PMID": 958633}
{"id": "pubmed23n0032_3177", "title": "Vitamin B2 status in athletes and the influence of riboflavin administration on neuromuscular irritability.", "content": "In order to obtain information on the vitamin B2 status in athletes in training, the following aspects were investigated: in one group of athletes, riboflavinuria at rest, during the autumn and winter months (mean values 23.8 and 9.45 mug/h, respectively); in the second group (n = 18), the activity of the nicotinamide-adenine-dinucleotide-phosphate-dependent red blood cell glutathione reductase (EC 1.6.4.2) and its activation by added flavin adenine dinucleotide. In 8 athletes, activation coefficients higher than 1.25 were found, indicating a biochemical deficiency of riboflavin. Erythrocyte transketolase (EC 2.2.1.1) was activated by more than 25% with added thiamine pyrophosphate in 12 cases. In muscle samples of 10 healthy patients (fresh fractures or removing of metal plates from old fractures), glutathione reductase (mean activity 0.846 +/- 0.458 U/g wet weight) was activated by more than 25% in 5 cases. Administration of 10 mg of riboflavin per os resulted in moderate lowering of neuromuscular irritability (musculi vastus medialis and lateralis quadricipitis) particularly for 0.1-msec rectangular stimuli.", "contents": "Vitamin B2 status in athletes and the influence of riboflavin administration on neuromuscular irritability. In order to obtain information on the vitamin B2 status in athletes in training, the following aspects were investigated: in one group of athletes, riboflavinuria at rest, during the autumn and winter months (mean values 23.8 and 9.45 mug/h, respectively); in the second group (n = 18), the activity of the nicotinamide-adenine-dinucleotide-phosphate-dependent red blood cell glutathione reductase (EC 1.6.4.2) and its activation by added flavin adenine dinucleotide. In 8 athletes, activation coefficients higher than 1.25 were found, indicating a biochemical deficiency of riboflavin. Erythrocyte transketolase (EC 2.2.1.1) was activated by more than 25% with added thiamine pyrophosphate in 12 cases. In muscle samples of 10 healthy patients (fresh fractures or removing of metal plates from old fractures), glutathione reductase (mean activity 0.846 +/- 0.458 U/g wet weight) was activated by more than 25% in 5 cases. Administration of 10 mg of riboflavin per os resulted in moderate lowering of neuromuscular irritability (musculi vastus medialis and lateralis quadricipitis) particularly for 0.1-msec rectangular stimuli.", "PMID": 958645}
{"id": "pubmed23n0032_3178", "title": "Circulating lipophages and aortic foam cells in experimental atherosclerosis of rabbits under altered reticuloendothelial activity.", "content": "Administration of Freund's adjuvant to cholesterol-fed rabbits inhibited the induction of atheroma in the aorta of rabbits when compared with cholesterol-fed indiaink-treated animals and cholesterol-fed saline-treated controls. Simultanously the blockade of the reticuloendothelial system by india ink caused a greater degree of atheromatous lesions in these rabbits. Smears of peripheral blood studied by light microscopy revealed the presence of circulating lipophages which were morphologically similar to the aortic foam cells isolated from the same animal. Isolation of mononuclear lipid-containing foam cells from aortic intima and the presence of a morphologically similar type of cell in circulating blood indicate that the blood might be the most likely source of intimal foam cells.", "contents": "Circulating lipophages and aortic foam cells in experimental atherosclerosis of rabbits under altered reticuloendothelial activity. Administration of Freund's adjuvant to cholesterol-fed rabbits inhibited the induction of atheroma in the aorta of rabbits when compared with cholesterol-fed indiaink-treated animals and cholesterol-fed saline-treated controls. Simultanously the blockade of the reticuloendothelial system by india ink caused a greater degree of atheromatous lesions in these rabbits. Smears of peripheral blood studied by light microscopy revealed the presence of circulating lipophages which were morphologically similar to the aortic foam cells isolated from the same animal. Isolation of mononuclear lipid-containing foam cells from aortic intima and the presence of a morphologically similar type of cell in circulating blood indicate that the blood might be the most likely source of intimal foam cells.", "PMID": 958646}
{"id": "pubmed23n0032_3179", "title": "Metabolism of liver triacylglycerols in rats tube-fed a threonine-devoid diet.", "content": "Rats tube-fed a diet devoid of threonine accumulated triacylglycerols in their livers, starting on the third day of the diet. The fatty acid composition of the accumulated lipid and the contribution of novo synthesized fatty acids to the lipid accumulation, as determined with tritiated water as a radioactive precursor for fatty acid synthesis, suggested that an increased hepatic de novo synthesis of fatty acids is not a major factor for the development of this liver lipid accumulation. The metabolism of intravenous injected 3H-oleic acid, the Triton-induced hyperlipemia and the activity of lipoprotein lipase in adipose tissue was also studied. None of these studies revealed any significant difference between the threonine-deficient and control rats. It is concluded that the hepatic triacylglycerol accumulation in the threonine-deficient rats does not result from any gross abnormality in the rate of liver triacylglycerol formation or secretion to the plasma. It is suggested that a possible causative mechanism is a derangement in the metabolism of the storage pool of liver triacylglycerols.", "contents": "Metabolism of liver triacylglycerols in rats tube-fed a threonine-devoid diet. Rats tube-fed a diet devoid of threonine accumulated triacylglycerols in their livers, starting on the third day of the diet. The fatty acid composition of the accumulated lipid and the contribution of novo synthesized fatty acids to the lipid accumulation, as determined with tritiated water as a radioactive precursor for fatty acid synthesis, suggested that an increased hepatic de novo synthesis of fatty acids is not a major factor for the development of this liver lipid accumulation. The metabolism of intravenous injected 3H-oleic acid, the Triton-induced hyperlipemia and the activity of lipoprotein lipase in adipose tissue was also studied. None of these studies revealed any significant difference between the threonine-deficient and control rats. It is concluded that the hepatic triacylglycerol accumulation in the threonine-deficient rats does not result from any gross abnormality in the rate of liver triacylglycerol formation or secretion to the plasma. It is suggested that a possible causative mechanism is a derangement in the metabolism of the storage pool of liver triacylglycerols.", "PMID": 958647}
{"id": "pubmed23n0032_3180", "title": "Biotin status and lipid metabolism in adult obese hypercholesterolemic inbred rats.", "content": "A statistically significant inverse association was generally found between plasma total lipid, cholesterol, or phospholipid and biotin status of 300-day-old male inbred BHE (IN-BHE) rats. Plasma, liver, and carcass lipid of both sexes generally had a significant direct association with liver lactate dehydrogenase activity; an inverse association in males resulted with improved biotin status. Elevated plasma lactate indicative of anaerobic glycolysis was found. It is proposed that an increased reductive environment - a consequence of accumulated NADH - could account for enhanced triglyceride synthesis and that this effect could explain the obesity in the IN-BHE rats. After the injection of 300 mug of biotin, plasma levels of lactate and pyruvate fell in male rats, indicating a stimulatory effect of biotin upon the oxidative pathways in these animals.", "contents": "Biotin status and lipid metabolism in adult obese hypercholesterolemic inbred rats. A statistically significant inverse association was generally found between plasma total lipid, cholesterol, or phospholipid and biotin status of 300-day-old male inbred BHE (IN-BHE) rats. Plasma, liver, and carcass lipid of both sexes generally had a significant direct association with liver lactate dehydrogenase activity; an inverse association in males resulted with improved biotin status. Elevated plasma lactate indicative of anaerobic glycolysis was found. It is proposed that an increased reductive environment - a consequence of accumulated NADH - could account for enhanced triglyceride synthesis and that this effect could explain the obesity in the IN-BHE rats. After the injection of 300 mug of biotin, plasma levels of lactate and pyruvate fell in male rats, indicating a stimulatory effect of biotin upon the oxidative pathways in these animals.", "PMID": 958648}
{"id": "pubmed23n0032_3181", "title": "Food intake, dietary supplements and survival time of scorbutic guinea pigs.", "content": "Manual presentation of a liquid scorbutogenic diet permitted a more complete characterisation of the terminal stages of scurvy in guinea pigs. A bioflavonoid preparation from orange peel, previously shown both to increase the tissue concentrations of ascorbic acid and to stimulate the growth of hypovitaminotic C guinea pigs, had no influence on the survival time of scorbutic guinea pigs. Tetrahydrofolic acid, reported to possess 40% of the activity of ascorbic acid in the protocollagen proline-hydroxylase system, also failed significantly to prolong the survival period. This latter finding could indicate that ascorbic acid has essential biochemical functions in addition to its involvement in the hydroxylation of proline and lysine.", "contents": "Food intake, dietary supplements and survival time of scorbutic guinea pigs. Manual presentation of a liquid scorbutogenic diet permitted a more complete characterisation of the terminal stages of scurvy in guinea pigs. A bioflavonoid preparation from orange peel, previously shown both to increase the tissue concentrations of ascorbic acid and to stimulate the growth of hypovitaminotic C guinea pigs, had no influence on the survival time of scorbutic guinea pigs. Tetrahydrofolic acid, reported to possess 40% of the activity of ascorbic acid in the protocollagen proline-hydroxylase system, also failed significantly to prolong the survival period. This latter finding could indicate that ascorbic acid has essential biochemical functions in addition to its involvement in the hydroxylation of proline and lysine.", "PMID": 958649}
{"id": "pubmed23n0032_3182", "title": "Effect of starvation on pituitary growth hormone cells and blood growth hormone and prolactin levels in the rat.", "content": "Growth hormone (GH) cells of rats were studied on days, 2, 4 and 7 of starvation. Immunoperoxidase staining for light microscopy confirmed the presence of GH in the pituitaries of all groups of animals. Electron microscopy revealed crinophagy in the cytoplasm of GH cells on days 4 and 7. By ultrastructural morphometry, volume density and the diameter of secretory granules in the cytoplasm of GH cells remained unchanged. Blood GH determinations showed a significant decrease on day 4 of the starvation period. On day 7 most of the values were in the range of the controls. Blood prolactin levels fell significantly on day 7. It appears that the pituitary is capable of secreting GH even in rats completely deprived of exogenous nutrients.", "contents": "Effect of starvation on pituitary growth hormone cells and blood growth hormone and prolactin levels in the rat. Growth hormone (GH) cells of rats were studied on days, 2, 4 and 7 of starvation. Immunoperoxidase staining for light microscopy confirmed the presence of GH in the pituitaries of all groups of animals. Electron microscopy revealed crinophagy in the cytoplasm of GH cells on days 4 and 7. By ultrastructural morphometry, volume density and the diameter of secretory granules in the cytoplasm of GH cells remained unchanged. Blood GH determinations showed a significant decrease on day 4 of the starvation period. On day 7 most of the values were in the range of the controls. Blood prolactin levels fell significantly on day 7. It appears that the pituitary is capable of secreting GH even in rats completely deprived of exogenous nutrients.", "PMID": 958650}
{"id": "pubmed23n0032_3183", "title": "Serum vitamin B12 levels in protein-malnourished children.", "content": "Serum vitamin B12 levels were assayed in 25 healthy and 35 protein-malnourished children aged 1-6 years. Serum proteins and haematocrit values of the children were also measured. Liver enlargement was estimated clinically. The mean serum vitamin B12 activity among protein-malnourished children was slightly higher than that for the control group. Serum vitamin B12 activity of the healthy Nigerian children was, however, high, and possible reasons for this are discussed in detail. There was some correlation between very high serum vitamin B12 levels among the protein-malnourished children and incidence of liver enlargement.", "contents": "Serum vitamin B12 levels in protein-malnourished children. Serum vitamin B12 levels were assayed in 25 healthy and 35 protein-malnourished children aged 1-6 years. Serum proteins and haematocrit values of the children were also measured. Liver enlargement was estimated clinically. The mean serum vitamin B12 activity among protein-malnourished children was slightly higher than that for the control group. Serum vitamin B12 activity of the healthy Nigerian children was, however, high, and possible reasons for this are discussed in detail. There was some correlation between very high serum vitamin B12 levels among the protein-malnourished children and incidence of liver enlargement.", "PMID": 958651}
{"id": "pubmed23n0032_3184", "title": "Adaptation of pancreatic amylase activity to enhanced parenteral carbohydrate intake in rats, and electron microscopic findings.", "content": "The present study records a rise of amylase activity in rat pancreatic tissue after a long-term administration of high carbohydrate diets (14% excess of glucose administered orally or subcutaneously as 30% glucose solution) in comparison to a normal diet. It was concluded that the pancreas obtains information on intestinal food composition along the humoral pathway. Electron microscopic investigations revealed no impairment of the acinar cells of the pancreas, but only changes that may be considered as a structural expression of the various phases of activity of the acinar cells.", "contents": "Adaptation of pancreatic amylase activity to enhanced parenteral carbohydrate intake in rats, and electron microscopic findings. The present study records a rise of amylase activity in rat pancreatic tissue after a long-term administration of high carbohydrate diets (14% excess of glucose administered orally or subcutaneously as 30% glucose solution) in comparison to a normal diet. It was concluded that the pancreas obtains information on intestinal food composition along the humoral pathway. Electron microscopic investigations revealed no impairment of the acinar cells of the pancreas, but only changes that may be considered as a structural expression of the various phases of activity of the acinar cells.", "PMID": 958652}
{"id": "pubmed23n0032_3185", "title": "The energetics of \"catch up\" growth.", "content": "Animals, prevented from growing and then refed a good diet ad libitum, growth faster than normal and this is called \"catch up\" growth. This experiment was designed to show whether this is achieved by eating more food or by a greater efficiency of energy utilisation. Groups of rats (either 3 or 5 weeks old) were maintained at constant weight for a month, either by reducing the quantity of a good diet or by reducing the proportion of dietary protein. Food intake and carcass gain were measured for a period of 6 days \"catch up\" growth and compared with the control growth of rats with the same starting weight. It is concluded that \"catch up\" growth is associated with increased energy intake and increased efficiency when expressed as food conversion and gross energetic efficiency. There are, however, specific age differences in the metabolism of \"catch up\" growth which may be due to variation in either the maintenance requirement or the efficiency of protein and fat synthesis. This experiment emphasizes the differences between the various available expressions of energy utilisation.", "contents": "The energetics of \"catch up\" growth. Animals, prevented from growing and then refed a good diet ad libitum, growth faster than normal and this is called \"catch up\" growth. This experiment was designed to show whether this is achieved by eating more food or by a greater efficiency of energy utilisation. Groups of rats (either 3 or 5 weeks old) were maintained at constant weight for a month, either by reducing the quantity of a good diet or by reducing the proportion of dietary protein. Food intake and carcass gain were measured for a period of 6 days \"catch up\" growth and compared with the control growth of rats with the same starting weight. It is concluded that \"catch up\" growth is associated with increased energy intake and increased efficiency when expressed as food conversion and gross energetic efficiency. There are, however, specific age differences in the metabolism of \"catch up\" growth which may be due to variation in either the maintenance requirement or the efficiency of protein and fat synthesis. This experiment emphasizes the differences between the various available expressions of energy utilisation.", "PMID": 958653}
{"id": "pubmed23n0032_3186", "title": "Influence of age and sex on plasma zinc levels in normal and diabetic individuals.", "content": "Plasma zinc levels were measured in both healthy and diabetic individuals having an age range of 10-93 years. No significant differences in plasma zinc concentrations were found between males and females in either healthy or diabetic individuals. Up to the age of 50 years, the mean plasma zinc of normal individuals remained relatively constant at 70 +/- 32 mug/100 ml (+/- 2 SE) after which the levels decreased. This decreasing pattern was absent in diabetics, whose plasma zinc of 65 +/- 32 mug/100 ml remained constant over the entire age range. For women on oral contraceptive agents, the mean plasma zinc was 59 +/- 18 mug/100 ml, which was significantly lower than that of controls.", "contents": "Influence of age and sex on plasma zinc levels in normal and diabetic individuals. Plasma zinc levels were measured in both healthy and diabetic individuals having an age range of 10-93 years. No significant differences in plasma zinc concentrations were found between males and females in either healthy or diabetic individuals. Up to the age of 50 years, the mean plasma zinc of normal individuals remained relatively constant at 70 +/- 32 mug/100 ml (+/- 2 SE) after which the levels decreased. This decreasing pattern was absent in diabetics, whose plasma zinc of 65 +/- 32 mug/100 ml remained constant over the entire age range. For women on oral contraceptive agents, the mean plasma zinc was 59 +/- 18 mug/100 ml, which was significantly lower than that of controls.", "PMID": 958654}
{"id": "pubmed23n0032_3187", "title": "Selenium in in vitamin-E-deficient diets and the occurrence of myopathy as a symptom of vitamin E deficiency.", "content": "The occurrence of myopathy in vitamin-E-deficient ducklings, which is used for the determination of the degree of vitamin E deficiency, is depending on the selenium content of the diet. The selenium content of a semi-synthetic diet and its constituents was determined by neutron activation analysis, which showed to be an adequately sensitive and precise method of analysis for selenium. The casein compound was the main source of the selenium in the diet. Myopathy occurred with diets containing about 50 ppb Se; diets containing about 100 ppb Se failed to induce any signs of myopathy.", "contents": "Selenium in in vitamin-E-deficient diets and the occurrence of myopathy as a symptom of vitamin E deficiency. The occurrence of myopathy in vitamin-E-deficient ducklings, which is used for the determination of the degree of vitamin E deficiency, is depending on the selenium content of the diet. The selenium content of a semi-synthetic diet and its constituents was determined by neutron activation analysis, which showed to be an adequately sensitive and precise method of analysis for selenium. The casein compound was the main source of the selenium in the diet. Myopathy occurred with diets containing about 50 ppb Se; diets containing about 100 ppb Se failed to induce any signs of myopathy.", "PMID": 958655}
{"id": "pubmed23n0032_3188", "title": "The influence of vitamin E deficiency and combined deficiency in vitamin E and polyunsaturated fatty acids on the biosynthesis and degradation of rat central nervous system myelin.", "content": "The ability to incorporate intracranially injected 14C-labelled leucine into central nervous system (CNS) myelin was studied in developing rats fed a control diet, a diet deficient in vitamin E and a diet deficient both in vitamin E and polyunsaturated fatty acids. The turnover of radioactivity incorporated into myelin and the distribution of radioactivity between the individual proteins of rat CNS myelin at various stages of the deficiency state was studied. Impaired myelin formation was found in cases of both types of deficiency. The level of incorporated radioactivity was raised by both types of deficiency throughout the experimental period. The mean half life of myelin radioactivity was found higher in combined deficient animals as compared to control and vitamin-E-deficient rats. The distribution of radioactivity between myelinproteins, separated by polyacrylamide gel electrophoresis, appeared identical in the three experimental groups.", "contents": "The influence of vitamin E deficiency and combined deficiency in vitamin E and polyunsaturated fatty acids on the biosynthesis and degradation of rat central nervous system myelin. The ability to incorporate intracranially injected 14C-labelled leucine into central nervous system (CNS) myelin was studied in developing rats fed a control diet, a diet deficient in vitamin E and a diet deficient both in vitamin E and polyunsaturated fatty acids. The turnover of radioactivity incorporated into myelin and the distribution of radioactivity between the individual proteins of rat CNS myelin at various stages of the deficiency state was studied. Impaired myelin formation was found in cases of both types of deficiency. The level of incorporated radioactivity was raised by both types of deficiency throughout the experimental period. The mean half life of myelin radioactivity was found higher in combined deficient animals as compared to control and vitamin-E-deficient rats. The distribution of radioactivity between myelinproteins, separated by polyacrylamide gel electrophoresis, appeared identical in the three experimental groups.", "PMID": 958656}
{"id": "pubmed23n0032_3189", "title": "Electroretinography and temporal aspects in macular dystrophy.", "content": "In a family with cone-rod dystrophy of autosomal recess inheritance, the diagnosis was established by characteristic abnormalities of the ERG, which demonstrates cone responses reduced in amplitude and delayed in implicit time, and rod delayed implicit time in two sisters with macular degeneration. The abnormalities of the implicit time are seen also in two other sisters of the propositi, who are clinically normal. The hypothesis that they are only capable to transmit the gene defect to their offspring is advanced.", "contents": "Electroretinography and temporal aspects in macular dystrophy. In a family with cone-rod dystrophy of autosomal recess inheritance, the diagnosis was established by characteristic abnormalities of the ERG, which demonstrates cone responses reduced in amplitude and delayed in implicit time, and rod delayed implicit time in two sisters with macular degeneration. The abnormalities of the implicit time are seen also in two other sisters of the propositi, who are clinically normal. The hypothesis that they are only capable to transmit the gene defect to their offspring is advanced.", "PMID": 958662}
{"id": "pubmed23n0032_3190", "title": "Contractile peripapillary staphyloma.", "content": "A 42-year-old woman had a contractile staphyloma involving the peripapillary area of the fundus of the left eye. The finding was related with high myopia of the same eye, which had a VA of 6/6 P. Except an enlargement of the blind spot no other finding was observed. The movement had no relation with any organic or non-organic rhythm.", "contents": "Contractile peripapillary staphyloma. A 42-year-old woman had a contractile staphyloma involving the peripapillary area of the fundus of the left eye. The finding was related with high myopia of the same eye, which had a VA of 6/6 P. Except an enlargement of the blind spot no other finding was observed. The movement had no relation with any organic or non-organic rhythm.", "PMID": 958663}
{"id": "pubmed23n0032_3191", "title": "[The syndrome of multiple basal cell nevoid epitheliomatosis].", "content": "The syndrome of multiple basal-celled naevoid epitheliomatosis is characterized by systeme affections of the skin, the bony skeleton, the endocrine glands, the C.N.S., and the eye and its adnexa. A paraneoplastic pathology causing the cutanious lesions and tumours secreting hormones or similar substances is postulated. A long bibliography is appended.", "contents": "[The syndrome of multiple basal cell nevoid epitheliomatosis]. The syndrome of multiple basal-celled naevoid epitheliomatosis is characterized by systeme affections of the skin, the bony skeleton, the endocrine glands, the C.N.S., and the eye and its adnexa. A paraneoplastic pathology causing the cutanious lesions and tumours secreting hormones or similar substances is postulated. A long bibliography is appended.", "PMID": 958664}
{"id": "pubmed23n0032_3192", "title": "Influence of estrogens on intraocular tension in the eyeballs of rabbits.", "content": "Iridocorneal angles from the eyes of female rabbits subjected to large doses of estrogens were examined under an electron scanning microscope. A temporary rise in intraocular tension was noted lasting up to 24 h after injection of estrogens, followed by a slow return to normal conditions. Histostructural changes of the iridicorneal angle were observed as widening of the marginal sector, enlarging of the dimple-shaped depressions and also increased number and narrowing of the micropores in the central part of the iridocorneal field. The authors postulate that estrogens affect the histostructure of the iridocorneal field and also stimulate the circulation of the aqueous fluid.", "contents": "Influence of estrogens on intraocular tension in the eyeballs of rabbits. Iridocorneal angles from the eyes of female rabbits subjected to large doses of estrogens were examined under an electron scanning microscope. A temporary rise in intraocular tension was noted lasting up to 24 h after injection of estrogens, followed by a slow return to normal conditions. Histostructural changes of the iridicorneal angle were observed as widening of the marginal sector, enlarging of the dimple-shaped depressions and also increased number and narrowing of the micropores in the central part of the iridocorneal field. The authors postulate that estrogens affect the histostructure of the iridocorneal field and also stimulate the circulation of the aqueous fluid.", "PMID": 958665}
{"id": "pubmed23n0032_3193", "title": "Ocular pathology in the elfin face syndrome (the Fanconi-Schlesinger type of idiopathic hypercalcaemia of infancy). Histochemical and ultrastructural study of a case.", "content": "The pathology, particularly the ocular pathology, is described in a 42-year-old mildly retarded man with a history of vomiting in infancy. He had an elfin-like face. He died having a pancreatic carcinoma. At autopsy, a supravalvular aortic stenosis and a hypoplastic aorta found clinically were confirmed. Stainings for calcium were positive in the aortic wall, the kidneys, the adrenals and the spleen. In the eyes, calcium was found in the corneal epithelium and endothelium, in corneal keratocytes and stroma, in conjunctival epithelium and in the sclera. Electron microscopy of the eyes revealed that calcium was deposited as hydroxyapatite intracellularly in aggregations of needle-like crystals and extracellularly as spherules morphologically different from the intracellular deposits. Although the serum values of calcium in this patient were normal during his adult life, the histopathological examination indicates an earlier period of raised serum calcium. We find it probable that he had idiopathic hypercalcaemia in infancy, thereby connecting this infantile condition with the elfin face and supravalvular aortic stenosis. In similar cases, the use of the above-mentioned technique is recommended for revealing abnormal calcium storage post-mortem and in vivo (by conjunctival biopsy) in order to substantiate the diagnosis of hypercalcaemia.", "contents": "Ocular pathology in the elfin face syndrome (the Fanconi-Schlesinger type of idiopathic hypercalcaemia of infancy). Histochemical and ultrastructural study of a case. The pathology, particularly the ocular pathology, is described in a 42-year-old mildly retarded man with a history of vomiting in infancy. He had an elfin-like face. He died having a pancreatic carcinoma. At autopsy, a supravalvular aortic stenosis and a hypoplastic aorta found clinically were confirmed. Stainings for calcium were positive in the aortic wall, the kidneys, the adrenals and the spleen. In the eyes, calcium was found in the corneal epithelium and endothelium, in corneal keratocytes and stroma, in conjunctival epithelium and in the sclera. Electron microscopy of the eyes revealed that calcium was deposited as hydroxyapatite intracellularly in aggregations of needle-like crystals and extracellularly as spherules morphologically different from the intracellular deposits. Although the serum values of calcium in this patient were normal during his adult life, the histopathological examination indicates an earlier period of raised serum calcium. We find it probable that he had idiopathic hypercalcaemia in infancy, thereby connecting this infantile condition with the elfin face and supravalvular aortic stenosis. In similar cases, the use of the above-mentioned technique is recommended for revealing abnormal calcium storage post-mortem and in vivo (by conjunctival biopsy) in order to substantiate the diagnosis of hypercalcaemia.", "PMID": 958667}
{"id": "pubmed23n0032_3194", "title": "Phototherapy of the cornea: some aspects to be considered.", "content": "To safeguard against and reduce possible harmful and undesired side effects of photon interaction with ocular structures during phototherapy of certain corneal diseases, precautionary measures should be taken. These include (1) Selection of the proper light source, strongly emitting in the effective spectral range. (2) Removal of potential harmful spectral bands which make no significant contribution to the therapeutic action. (3) Determination of the most optimal exposure energy (W/cm2). (4) Selection of the most optimal time-dose relationship. (5) Selection of the proper interval between vital staining and radiation exposure and considering possible repetition of therapeutic exposures. (6) Careful calibration of the light source to be used. (7) Adequate eye examination for visual acuity, pupillary diameter, signs of intraocular inflammation, mainly iritis with anterior chamber activity, and possible fundus pathology with special attention to maculopathy. These findings should be properly recorded before, during and after the period of phototherapy.", "contents": "Phototherapy of the cornea: some aspects to be considered. To safeguard against and reduce possible harmful and undesired side effects of photon interaction with ocular structures during phototherapy of certain corneal diseases, precautionary measures should be taken. These include (1) Selection of the proper light source, strongly emitting in the effective spectral range. (2) Removal of potential harmful spectral bands which make no significant contribution to the therapeutic action. (3) Determination of the most optimal exposure energy (W/cm2). (4) Selection of the most optimal time-dose relationship. (5) Selection of the proper interval between vital staining and radiation exposure and considering possible repetition of therapeutic exposures. (6) Careful calibration of the light source to be used. (7) Adequate eye examination for visual acuity, pupillary diameter, signs of intraocular inflammation, mainly iritis with anterior chamber activity, and possible fundus pathology with special attention to maculopathy. These findings should be properly recorded before, during and after the period of phototherapy.", "PMID": 958668}
{"id": "pubmed23n0032_3195", "title": "The incidence of herpetic keratitis among population.", "content": "The author reports of a relatively little known aspects of herpetic keratitis in man, and its incidence among the population. The incidence of primary herpetic keratitis is 1:8,000 to 1:24,000 while for relapsing herpetic keratitis it amounts to 1:1,653. The relationship of incidence between primary and relapsing herpetic keratitis is 1:5 to 1:14. These achievements have their practical values from the clinical and social aspect.", "contents": "The incidence of herpetic keratitis among population. The author reports of a relatively little known aspects of herpetic keratitis in man, and its incidence among the population. The incidence of primary herpetic keratitis is 1:8,000 to 1:24,000 while for relapsing herpetic keratitis it amounts to 1:1,653. The relationship of incidence between primary and relapsing herpetic keratitis is 1:5 to 1:14. These achievements have their practical values from the clinical and social aspect.", "PMID": 958673}
{"id": "pubmed23n0032_3196", "title": "Bullous keratopathy following lens extraction in eyes with acute glaucoma due to intumescent cataract.", "content": "Nine cases are presented, six of them in detail, which suggest that there is a high risk of bullous keratopathy developing when the lens is removed for the treatment of acute glaucoma due to an intumescent cataract. In some cases the keratopathy is due to detachment of Descemet's membrane from the cornea. Acute glaucoma due to an intumescent cataract should be treated medically or by peripheral iridectomy. The lens should be removed only after the intraocular pressure has been normal and the cornea free of edema for at least 6 weeks.", "contents": "Bullous keratopathy following lens extraction in eyes with acute glaucoma due to intumescent cataract. Nine cases are presented, six of them in detail, which suggest that there is a high risk of bullous keratopathy developing when the lens is removed for the treatment of acute glaucoma due to an intumescent cataract. In some cases the keratopathy is due to detachment of Descemet's membrane from the cornea. Acute glaucoma due to an intumescent cataract should be treated medically or by peripheral iridectomy. The lens should be removed only after the intraocular pressure has been normal and the cornea free of edema for at least 6 weeks.", "PMID": 958674}
{"id": "pubmed23n0032_3197", "title": "The effect of orbital irradiation on the survival rate of patients with choroidal melanoma.", "content": "Orbital irradiation with 6,000 rad after enucleation of patients suffering from choroidal melanoma resulted in a survival rate nearly twice as high after 5 years as without any additional radiotherapy.", "contents": "The effect of orbital irradiation on the survival rate of patients with choroidal melanoma. Orbital irradiation with 6,000 rad after enucleation of patients suffering from choroidal melanoma resulted in a survival rate nearly twice as high after 5 years as without any additional radiotherapy.", "PMID": 958675}
{"id": "pubmed23n0032_3198", "title": "[Cryotherapy of eyelid and conjunctival tumors].", "content": "Report is given of cryobiological principles, technique and results of cryotherapy for eyelid or conjunctival tumors in 181 cases. In the majority of cases they were basaliomas. In all cases the therapy was successful. No eyelid contractions or tear duct stenosis were noted. The follow-up of 4,5 years showed only 2 recidives. Histologically, cryonecrosis showed capillary ectasis with hyperemia.", "contents": "[Cryotherapy of eyelid and conjunctival tumors]. Report is given of cryobiological principles, technique and results of cryotherapy for eyelid or conjunctival tumors in 181 cases. In the majority of cases they were basaliomas. In all cases the therapy was successful. No eyelid contractions or tear duct stenosis were noted. The follow-up of 4,5 years showed only 2 recidives. Histologically, cryonecrosis showed capillary ectasis with hyperemia.", "PMID": 958676}
{"id": "pubmed23n0032_3199", "title": "Fractures about the knee in children.", "content": "The seriousness of the consequences of growth and angular disturbances about the knee that occur as a result of injuries in childhood cannot be overemphasized. The resulting limb length discrepancy is well known to the office practitioner, who sees these patients presenting with back problems during adulthood. Normal knee motion and stability are prime prerequisites for normal gait, and loss of knee motion as a determinant of gait can increase the energy requirement up to 300-fold. The management of these injuries presented has produced good results for the author. The author strongly recommends careful follow-up in metaphyseal fractures to guard against the development of angular deformities, the anatomical reduction of all intra-articular fractures, and x-ray examination of the hip in patients in the four to 15 year age group who have sustained minimal or insignificant trauma and who complain of pain about the knee.", "contents": "Fractures about the knee in children. The seriousness of the consequences of growth and angular disturbances about the knee that occur as a result of injuries in childhood cannot be overemphasized. The resulting limb length discrepancy is well known to the office practitioner, who sees these patients presenting with back problems during adulthood. Normal knee motion and stability are prime prerequisites for normal gait, and loss of knee motion as a determinant of gait can increase the energy requirement up to 300-fold. The management of these injuries presented has produced good results for the author. The author strongly recommends careful follow-up in metaphyseal fractures to guard against the development of angular deformities, the anatomical reduction of all intra-articular fractures, and x-ray examination of the hip in patients in the four to 15 year age group who have sustained minimal or insignificant trauma and who complain of pain about the knee.", "PMID": 958679}
{"id": "pubmed23n0032_3200", "title": "Fractures of the humerus in children.", "content": "Fractures of the humerus above the distal epiphysis are not common fractures in children. Treatment should be directed toward maintenance of alignment with healing expected without fail. Contrary to experience with adults with the same fractures, children seldom have residual functional or cosmetic problems if one aligns the fractures and protects the healing process.", "contents": "Fractures of the humerus in children. Fractures of the humerus above the distal epiphysis are not common fractures in children. Treatment should be directed toward maintenance of alignment with healing expected without fail. Contrary to experience with adults with the same fractures, children seldom have residual functional or cosmetic problems if one aligns the fractures and protects the healing process.", "PMID": 958682}
{"id": "pubmed23n0032_3201", "title": "Dislocations of the upper extremity in children.", "content": "Traumatic dislocations and subluxations of the upper extremity in children have been surveyed. The elbow joint is most commonly dislocated and is dislocated more frequently in children than in adults. Severe neurovascular injury may be associated with dislocation of this joint. The most common subluxation is that of the radial head, secondary to a \"pulled elbow.\" Otherwise dislocations and subluxations of the upper extremity are uncommon in children, apparently because of the relative weakness of the epiphyseal plate. Most of these injuries are the result of predictable indirect forces. Principles of reduction and immobilization are based on the unique anatomic structure of the joint involved and the known anatomic disruption resulting from the trauma.", "contents": "Dislocations of the upper extremity in children. Traumatic dislocations and subluxations of the upper extremity in children have been surveyed. The elbow joint is most commonly dislocated and is dislocated more frequently in children than in adults. Severe neurovascular injury may be associated with dislocation of this joint. The most common subluxation is that of the radial head, secondary to a \"pulled elbow.\" Otherwise dislocations and subluxations of the upper extremity are uncommon in children, apparently because of the relative weakness of the epiphyseal plate. Most of these injuries are the result of predictable indirect forces. Principles of reduction and immobilization are based on the unique anatomic structure of the joint involved and the known anatomic disruption resulting from the trauma.", "PMID": 958684}
{"id": "pubmed23n0032_3202", "title": "Fractures and dislocations of the cervical spine in children.", "content": "Correct diagnosis of fractures and dislocations of the cervical spine in children requires an awareness of the pseudosubluxation and other normal variants noted in x-ray films of patients in this age group. Fractures of the odontoid process occur frequently and almost always can be treated by closed reduction and external fixation until union occurs. Fractures of the lower cervical spine are difficult to detect roentgenographically, and x-ray changes can belie the severity of soft tissue injury and cord trauma. Instability tends to persist in adolescent patients after cervical spine injury because of the combination of epiphyseal and posterior ligamentous disruption. Neoplastic, inflammatory, and congenital lesions render the cervical spine vulnerable to injury and can permit major damage to result from minor stress.", "contents": "Fractures and dislocations of the cervical spine in children. Correct diagnosis of fractures and dislocations of the cervical spine in children requires an awareness of the pseudosubluxation and other normal variants noted in x-ray films of patients in this age group. Fractures of the odontoid process occur frequently and almost always can be treated by closed reduction and external fixation until union occurs. Fractures of the lower cervical spine are difficult to detect roentgenographically, and x-ray changes can belie the severity of soft tissue injury and cord trauma. Instability tends to persist in adolescent patients after cervical spine injury because of the combination of epiphyseal and posterior ligamentous disruption. Neoplastic, inflammatory, and congenital lesions render the cervical spine vulnerable to injury and can permit major damage to result from minor stress.", "PMID": 958685}
{"id": "pubmed23n0032_3203", "title": "Fractures of the dorsal and lumbar spine.", "content": "A study of 60 children with thoracic and lumbar spine injuries indicates that the child's response to trauma is unique. It is concluded that the potential for growth and development and the presence of healthy tissues separate these injuries from comparable injuries in adults. The epiphyseal growth partly or completely restores vertebral body height, leads to a mild spinal deformity in stable injuries, and can contribute to rapidly progressive spinal deformity in unstable injuries with or without paralysis. Healthy intervertebral discs account for the multiple compression fractures and lack of long term degenerative changes of the spine. On the basis of this review, a practical approach to patient care is presented.", "contents": "Fractures of the dorsal and lumbar spine. A study of 60 children with thoracic and lumbar spine injuries indicates that the child's response to trauma is unique. It is concluded that the potential for growth and development and the presence of healthy tissues separate these injuries from comparable injuries in adults. The epiphyseal growth partly or completely restores vertebral body height, leads to a mild spinal deformity in stable injuries, and can contribute to rapidly progressive spinal deformity in unstable injuries with or without paralysis. Healthy intervertebral discs account for the multiple compression fractures and lack of long term degenerative changes of the spine. On the basis of this review, a practical approach to patient care is presented.", "PMID": 958686}
{"id": "pubmed23n0032_3204", "title": "Fractures of the femoral diaphysis in children.", "content": "Bryant's traction in children in whom the proper prerequisites are present, 90-90 traction for proximal third fractures in children over three, all shaft fractures in children between three and 10 years of age, and split Russell skeletal traction for those over 10 years of age are effective methods for treating femural fractures. Attention to detail is necessary to correct angulation and to obtain and maintain the appropriate amount of over-riding based upon age. Excessive over-riding, distraction, and unreasonable angulation cause functional disability that may require corrective surgery.", "contents": "Fractures of the femoral diaphysis in children. Bryant's traction in children in whom the proper prerequisites are present, 90-90 traction for proximal third fractures in children over three, all shaft fractures in children between three and 10 years of age, and split Russell skeletal traction for those over 10 years of age are effective methods for treating femural fractures. Attention to detail is necessary to correct angulation and to obtain and maintain the appropriate amount of over-riding based upon age. Excessive over-riding, distraction, and unreasonable angulation cause functional disability that may require corrective surgery.", "PMID": 958689}
{"id": "pubmed23n0032_3205", "title": "Peripheral nerve injuries in children.", "content": "Recovery after peripheral nerve injuries in children is more complete than in adults and is inversely related to the age of the patient. The prognosis for the return of sensation following laceration of the median, ulnar, or digital nerve depends upon recovery of two point discrimination (in millimeters approximately equal to the child's age) at the time of nerve repair. The better results in children probably reflect the greater adaptability of the immature central nervous system to the nerve injury. Operative exploration of an open wound when there is a potential for nerve injury in an uncooperative child is the only sure way of determining the status of the nerves. Primary repair of cleanly divided nerves in tidy wounds is advocated if it can be done competently. Secondary repair is indicated for avulsion injuries, gunshot wounds, crush injuries, and human or animal bites. Delicate, atraumatic technique and accurate repair of the divided nerve are stressed. The more exacting technique of funicular repair may yield better results. Interfascicular cable grafting is a new and useful alternative to extensive mobilization in closing nerve gaps. Nonoperative treatment of nerve injuries associated with closed fractures is advocated unless there are no signs of nerve regeneration in two to three months. Obstetrical brachial plexus injuries of the upper plexus carry a better prognosis than lower plexus or total plexus injury. Early range of motion exercises to prevent contractures are stressed. Maximal recovery takes place within two years. The acute nerve compression syndrome should be considered an emergency and may require surgical decompression if it is severe and if rapid return of function does not occur following reduction of the fracture.", "contents": "Peripheral nerve injuries in children. Recovery after peripheral nerve injuries in children is more complete than in adults and is inversely related to the age of the patient. The prognosis for the return of sensation following laceration of the median, ulnar, or digital nerve depends upon recovery of two point discrimination (in millimeters approximately equal to the child's age) at the time of nerve repair. The better results in children probably reflect the greater adaptability of the immature central nervous system to the nerve injury. Operative exploration of an open wound when there is a potential for nerve injury in an uncooperative child is the only sure way of determining the status of the nerves. Primary repair of cleanly divided nerves in tidy wounds is advocated if it can be done competently. Secondary repair is indicated for avulsion injuries, gunshot wounds, crush injuries, and human or animal bites. Delicate, atraumatic technique and accurate repair of the divided nerve are stressed. The more exacting technique of funicular repair may yield better results. Interfascicular cable grafting is a new and useful alternative to extensive mobilization in closing nerve gaps. Nonoperative treatment of nerve injuries associated with closed fractures is advocated unless there are no signs of nerve regeneration in two to three months. Obstetrical brachial plexus injuries of the upper plexus carry a better prognosis than lower plexus or total plexus injury. Early range of motion exercises to prevent contractures are stressed. Maximal recovery takes place within two years. The acute nerve compression syndrome should be considered an emergency and may require surgical decompression if it is severe and if rapid return of function does not occur following reduction of the fracture.", "PMID": 958691}
{"id": "pubmed23n0032_3206", "title": "Treatment of tendon injuries in children.", "content": "Tendon injuries in the child's hand can be repaired primarily with better results than in the adult; this is true at all levels, including the digital sheath. In less than ideal circumstances, secondary conventional tendon grafting also provides predictable satisfactory results and can also salvage a failed primary repair. In children under the age of six years with digital flexor tendon repairs, the limb must be immobilized above the elbow. In the cooperative child over the age of six years with a primary repair of a lacerated flexor tendon, the dorsal splint with rubber band traction as described by Kleinert is recommended. Preoperative diagnosis of tendon injuries in the young child is difficult. If there is any doubt after observing the child using his hand, the wound must be explored and tendons and nerves identified to ensure their integrity. Even though the results of tendon repair in children are better than in adults, the structures are small, technique must be meticulous, and magnification must be used. Specially trained hand surgeons experienced in primary tendon repair and tendon grafting should perform these procedures, for this elegant structure deserves the finest attention.", "contents": "Treatment of tendon injuries in children. Tendon injuries in the child's hand can be repaired primarily with better results than in the adult; this is true at all levels, including the digital sheath. In less than ideal circumstances, secondary conventional tendon grafting also provides predictable satisfactory results and can also salvage a failed primary repair. In children under the age of six years with digital flexor tendon repairs, the limb must be immobilized above the elbow. In the cooperative child over the age of six years with a primary repair of a lacerated flexor tendon, the dorsal splint with rubber band traction as described by Kleinert is recommended. Preoperative diagnosis of tendon injuries in the young child is difficult. If there is any doubt after observing the child using his hand, the wound must be explored and tendons and nerves identified to ensure their integrity. Even though the results of tendon repair in children are better than in adults, the structures are small, technique must be meticulous, and magnification must be used. Specially trained hand surgeons experienced in primary tendon repair and tendon grafting should perform these procedures, for this elegant structure deserves the finest attention.", "PMID": 958692}
{"id": "pubmed23n0032_3207", "title": "The role of orthopedist in child abuse and neglect.", "content": "The diagnosis of child abuse is contingent upon having a high index of suspicion. Orthopedic surgeons and other surgical specialists should be more involved in a team approach for prevention, detection and management in cases of child abuse in the hope of breaking the cycle of this mutilating and often fatal tragedy.", "contents": "The role of orthopedist in child abuse and neglect. The diagnosis of child abuse is contingent upon having a high index of suspicion. Orthopedic surgeons and other surgical specialists should be more involved in a team approach for prevention, detection and management in cases of child abuse in the hope of breaking the cycle of this mutilating and often fatal tragedy.", "PMID": 958693}
{"id": "pubmed23n0032_3208", "title": "[Ultrastructure of the epithelium of the midgut in nymphs of Hyalomma asiaticum (Acarina, Ixodidae) ticks during feeding].", "content": "The midgut epithelium of H. asiaticum nymphs was studied by means of electron microscopy. It has been shown that during feeding the midgut epithelium of the above nymphs consists of three types of cells: reserve, secretory and digestive ones. There have been revealed two types of the digestive cells. The cells of the first type are capable for two forms of blood endocytosis: phagocytosis and pinocytosis. During feeding in these cells takes place fast intracellular digestion. This type of cells dominates at the beginning of feeding. The second type of digestive cells prevailing at the end of feeding is represented by specialized cells consuming only haemoglobin. A certain part of these cells preserves for a long time an intracellular reserve of haemoglobin.", "contents": "[Ultrastructure of the epithelium of the midgut in nymphs of Hyalomma asiaticum (Acarina, Ixodidae) ticks during feeding]. The midgut epithelium of H. asiaticum nymphs was studied by means of electron microscopy. It has been shown that during feeding the midgut epithelium of the above nymphs consists of three types of cells: reserve, secretory and digestive ones. There have been revealed two types of the digestive cells. The cells of the first type are capable for two forms of blood endocytosis: phagocytosis and pinocytosis. During feeding in these cells takes place fast intracellular digestion. This type of cells dominates at the beginning of feeding. The second type of digestive cells prevailing at the end of feeding is represented by specialized cells consuming only haemoglobin. A certain part of these cells preserves for a long time an intracellular reserve of haemoglobin.", "PMID": 958714}
{"id": "pubmed23n0032_3209", "title": "[Sublethal action and aftereffect of DDT on Dermacentor silvarum 01. (Acarina, Ixodidae)].", "content": "Observations were conducted on the processes of feeding and oviposition in the females of Dermacentor silvarum poisoned with DDT, the ancestors of which had been subjected to this acaricide and had overcome the poisoning due to the feeding. The rise in the sensitivity to DDT in the females of the second generation and a number of pathological changes both in feeding females and in their progeny were noted.", "contents": "[Sublethal action and aftereffect of DDT on Dermacentor silvarum 01. (Acarina, Ixodidae)]. Observations were conducted on the processes of feeding and oviposition in the females of Dermacentor silvarum poisoned with DDT, the ancestors of which had been subjected to this acaricide and had overcome the poisoning due to the feeding. The rise in the sensitivity to DDT in the females of the second generation and a number of pathological changes both in feeding females and in their progeny were noted.", "PMID": 958715}
{"id": "pubmed23n0032_3210", "title": "[Study of the inhibitory action of phenothiazine on carbohydrate metabolism in Mecistocirrus digitatus (Nematoda: Rhabditida)].", "content": "The presence of phenothiazine in incubation medium of M. digitatus exerted the following effects: the increase of glycogene splitting and lactic acid excretion in aerobic conditions; the increase of acetic acid excretion and the decrease of alpha-methylbutyric acid excretion; glycolysis process inhibition in homogenates and supernatants during incubation with glucose. However, at phenothiazine incubation with FDF glycolysis intensity was unaffected.", "contents": "[Study of the inhibitory action of phenothiazine on carbohydrate metabolism in Mecistocirrus digitatus (Nematoda: Rhabditida)]. The presence of phenothiazine in incubation medium of M. digitatus exerted the following effects: the increase of glycogene splitting and lactic acid excretion in aerobic conditions; the increase of acetic acid excretion and the decrease of alpha-methylbutyric acid excretion; glycolysis process inhibition in homogenates and supernatants during incubation with glucose. However, at phenothiazine incubation with FDF glycolysis intensity was unaffected.", "PMID": 958716}
{"id": "pubmed23n0032_3211", "title": "[Study of the cumulation, desorption and mechanism of action of organochlorine pesticides on fish cestodes].", "content": "The distribution of residues of DDT and its metabolites (DDD and DDE) in tissues of Triaenophorus nodulosus and its host was studied. It has been noted that cestodes can accumulate chlororganic substances in great quantity. The ability of Bothriocephalus gowkongensis has been established to dechlorate pp-DDT and to excrete pp-, op- and mp-DDD from the organism. Pp-DDT decreases the glycogene content and causes the rise of amylolytic activity in the above cestodes in acute tests. The effect of pp-DDT on the locomotor activity of cestodes is of two-phase character. The first one is associated with the muscular influence, the second one-- with neurotrophic effect. A high toxicity of mp-DDD )0.1 mg/l) for the locomotor activity of cestodes as compared with the effect of pp- and op-DDD was revealed.", "contents": "[Study of the cumulation, desorption and mechanism of action of organochlorine pesticides on fish cestodes]. The distribution of residues of DDT and its metabolites (DDD and DDE) in tissues of Triaenophorus nodulosus and its host was studied. It has been noted that cestodes can accumulate chlororganic substances in great quantity. The ability of Bothriocephalus gowkongensis has been established to dechlorate pp-DDT and to excrete pp-, op- and mp-DDD from the organism. Pp-DDT decreases the glycogene content and causes the rise of amylolytic activity in the above cestodes in acute tests. The effect of pp-DDT on the locomotor activity of cestodes is of two-phase character. The first one is associated with the muscular influence, the second one-- with neurotrophic effect. A high toxicity of mp-DDD )0.1 mg/l) for the locomotor activity of cestodes as compared with the effect of pp- and op-DDD was revealed.", "PMID": 958717}
{"id": "pubmed23n0032_3212", "title": "[Genus Polylabris Euzet et Cauwet, 1967 and some related species of microcotylids (Monogenoidea: Microcotylidae)].", "content": "The species P. diplodi Euzet et Cauwet, 1967, P. kuhliae (Yamaguti, 1967), P. maomao (Yamaguti, 1968), P. acanthogobii (Yamaguti, 1940), P. gerres (Sandars, 1944), P. tubicirrus (Paperna et Kohn, 1964) should be assigned to the genus Polylabris Euzet et Cauwet, 1967 (=Prostatomicrocotyla Yamaguti, 1968). Undoubtedly, \"Microcotyle sillaginae Woolcock, 1936\" in sensu Gupta and Khullar, 1968, and very likely the true Microcotyle sillaginae Woolcock, 1936 belong to the same genus. P. gerres (Machida, 1973) is a synonym and at the same time a homonym of P. gerres (Sandars, 1944). A new species, Polylabris acanthopagri sp. n., is described and a new genus, Polylabroides, with a single species, P. biungulatus gen. et sp. n., is proposed.", "contents": "[Genus Polylabris Euzet et Cauwet, 1967 and some related species of microcotylids (Monogenoidea: Microcotylidae)]. The species P. diplodi Euzet et Cauwet, 1967, P. kuhliae (Yamaguti, 1967), P. maomao (Yamaguti, 1968), P. acanthogobii (Yamaguti, 1940), P. gerres (Sandars, 1944), P. tubicirrus (Paperna et Kohn, 1964) should be assigned to the genus Polylabris Euzet et Cauwet, 1967 (=Prostatomicrocotyla Yamaguti, 1968). Undoubtedly, \"Microcotyle sillaginae Woolcock, 1936\" in sensu Gupta and Khullar, 1968, and very likely the true Microcotyle sillaginae Woolcock, 1936 belong to the same genus. P. gerres (Machida, 1973) is a synonym and at the same time a homonym of P. gerres (Sandars, 1944). A new species, Polylabris acanthopagri sp. n., is described and a new genus, Polylabroides, with a single species, P. biungulatus gen. et sp. n., is proposed.", "PMID": 958718}
{"id": "pubmed23n0032_3213", "title": "[Microsporidiosis and coccidiosis, protozoan diseases of flour beetles (Coleoptera, Tenebrionidae)].", "content": "Tribolium destructor and T. confusum diseases caused by the fat body of the parasites Nosema whitei and Adelina tribolii were investigated. Life span and weight dynamics were examined at different temperatures and infection conditions. Infected females of T. confusum lay a less number of eggs. The gas produced by stink glands of T. destructor may inactivate the spores of N. whitei but does not affect those of A. tribolii.", "contents": "[Microsporidiosis and coccidiosis, protozoan diseases of flour beetles (Coleoptera, Tenebrionidae)]. Tribolium destructor and T. confusum diseases caused by the fat body of the parasites Nosema whitei and Adelina tribolii were investigated. Life span and weight dynamics were examined at different temperatures and infection conditions. Infected females of T. confusum lay a less number of eggs. The gas produced by stink glands of T. destructor may inactivate the spores of N. whitei but does not affect those of A. tribolii.", "PMID": 958720}
{"id": "pubmed23n0032_3214", "title": "[Adaptations of the genital system of Metastrongylus elongatus in relation to the conditions of postembyonic development].", "content": "A topography of the genital system of Metastrongylus elongatus is described in comparison with the genital system of Dictyocaulus viviparus. It is established that in these species of helminths the genital tubes have a similar location but in Metastrongylus elongatus takes place an uterus lenthening caused by the transference of the genital orifice to the hind end of the body. Its total length is 2.5 as long as the body length of the worm. This phenomenon is regarded as an adaptation providing this species a possibility to produce eggs which can for a long time exist in the environment.", "contents": "[Adaptations of the genital system of Metastrongylus elongatus in relation to the conditions of postembyonic development]. A topography of the genital system of Metastrongylus elongatus is described in comparison with the genital system of Dictyocaulus viviparus. It is established that in these species of helminths the genital tubes have a similar location but in Metastrongylus elongatus takes place an uterus lenthening caused by the transference of the genital orifice to the hind end of the body. Its total length is 2.5 as long as the body length of the worm. This phenomenon is regarded as an adaptation providing this species a possibility to produce eggs which can for a long time exist in the environment.", "PMID": 958719}
{"id": "pubmed23n0032_3215", "title": "[Swarming of mosquitoes of the genus Aedes (Culicidae) in the forest tundra].", "content": "Observations on the swarming of mosquitoes were conducted in the flood plain forest of South Yamal (67 degrees N.L.). The individuals of A. communis made 83 to 94% of the swarming males. Their number in swarms varied from 30 to 800 specimens. The dynamics of the swarming males number depends on the rate of their emergence from the pupae. At the mass emergence the number of swarming males was 3 times higher though the increase in the general size of the population was negligible. Synchronization of the swarming time of males was also favoured by warm windless weather.", "contents": "[Swarming of mosquitoes of the genus Aedes (Culicidae) in the forest tundra]. Observations on the swarming of mosquitoes were conducted in the flood plain forest of South Yamal (67 degrees N.L.). The individuals of A. communis made 83 to 94% of the swarming males. Their number in swarms varied from 30 to 800 specimens. The dynamics of the swarming males number depends on the rate of their emergence from the pupae. At the mass emergence the number of swarming males was 3 times higher though the increase in the general size of the population was negligible. Synchronization of the swarming time of males was also favoured by warm windless weather.", "PMID": 958723}
{"id": "pubmed23n0032_3216", "title": "Genuine giant-cell tumour of bone; a combined cytological, histopathological and ultrastructural study.", "content": "Three patients with a giant-cell tumour of bone were studied clinically, roentgenologically and morphologically, using cytological, histopathological and electron microscopic methods. The tumours were composed of giant cells possessing a great number of mitochondria and stromal cells exhibiting prominent endoplasmic reticulum and Golgi complex. Mitotic activity was only found among the stromal cells. Autophagic vacuoles, lysosomal bodies and degenerative changes of varying severity were found mainly or exclusively among the giant cells, which may indicate that these cells represent an involutionary form of tumour cell. The giant-cell tumours of bone are believed to arise from undifferentiated cells of the bone marrow. Fine-needle aspiration biopsy represents a valuable complement to histopathological examination in the identification of giant cell lesions and diagnosis of genuine cell tumours of bone.", "contents": "Genuine giant-cell tumour of bone; a combined cytological, histopathological and ultrastructural study. Three patients with a giant-cell tumour of bone were studied clinically, roentgenologically and morphologically, using cytological, histopathological and electron microscopic methods. The tumours were composed of giant cells possessing a great number of mitochondria and stromal cells exhibiting prominent endoplasmic reticulum and Golgi complex. Mitotic activity was only found among the stromal cells. Autophagic vacuoles, lysosomal bodies and degenerative changes of varying severity were found mainly or exclusively among the giant cells, which may indicate that these cells represent an involutionary form of tumour cell. The giant-cell tumours of bone are believed to arise from undifferentiated cells of the bone marrow. Fine-needle aspiration biopsy represents a valuable complement to histopathological examination in the identification of giant cell lesions and diagnosis of genuine cell tumours of bone.", "PMID": 958730}
{"id": "pubmed23n0032_3217", "title": "Ethidium bromide and hepatic mitochondrial structure in mice. A morphometric analysis.", "content": "Nine days after receiving a single injection of ethidium bromide - an inhibitor of mitochondrial DNA and its synthesis - mice were found to have enlarged mitochondria which were also reduced in number. The morphometric study revealed an increase of the mean mitochondrial volume, as well as an enlargement of the surface area of the mitochondrial inner membrane. However, the surface of the inner  and outer membrane per unit volume of mitochondrion remained unchanged. These morphometric findings suggest mitochondrial growth, since mitochondrial inner membranes can be synthesized even in the presence of DNA-inhibiting ethidium bromide. In addition, morphometric analysis enables us to estimate the mean life span of hepatic mitochondria. In conclusion we may assume that since ethidium bromide induces not only a reduction of mitochondrial division but also an increase in the hepatic mitochondrial volume and inner membrane, the mitochondrial genome possibly fulfills a regulatory role in the mitochondrial and cytoplasmic systems for protein synthesis.", "contents": "Ethidium bromide and hepatic mitochondrial structure in mice. A morphometric analysis. Nine days after receiving a single injection of ethidium bromide - an inhibitor of mitochondrial DNA and its synthesis - mice were found to have enlarged mitochondria which were also reduced in number. The morphometric study revealed an increase of the mean mitochondrial volume, as well as an enlargement of the surface area of the mitochondrial inner membrane. However, the surface of the inner  and outer membrane per unit volume of mitochondrion remained unchanged. These morphometric findings suggest mitochondrial growth, since mitochondrial inner membranes can be synthesized even in the presence of DNA-inhibiting ethidium bromide. In addition, morphometric analysis enables us to estimate the mean life span of hepatic mitochondria. In conclusion we may assume that since ethidium bromide induces not only a reduction of mitochondrial division but also an increase in the hepatic mitochondrial volume and inner membrane, the mitochondrial genome possibly fulfills a regulatory role in the mitochondrial and cytoplasmic systems for protein synthesis.", "PMID": 958731}
{"id": "pubmed23n0032_3218", "title": "[Helminth fauna of Bering Sea pinnipeds from Karaginskiy Bay].", "content": "The helminthological investigations were conducted from May 6 to May 28, 1972 in the Karaginsk Gulf of the Bering Sea. The helminths were collected from 124 specimens of Phoca vitulina largha, 9 specimens of Erignathus barbatus nauticus, 9 specimens of Pusa hispida krascheninnikovi and 9 specimens of Histriophoca fasciata. In all, 23 species, 2 immature and 1 larval form of helminths were recorded. The data are given on the localization of helminths, intensity and extensity of their invasion, pathogenic effect on the host. The age dynamics of the helminths from Phoca vitulina largha was studied. For the first time are reported the trematode Orthosplanchnus pygmaeus, the cestode Diplogonoporus tetrapterus and nematodes Aniskis simplex, Anisakis sp. and Parafilaroides krascheninnikovi in Phoca vitulina largha and the nematodes Anisakis sp. and Phocascaris cystophorae in Pusa hispida krascheninnikovi.", "contents": "[Helminth fauna of Bering Sea pinnipeds from Karaginskiy Bay]. The helminthological investigations were conducted from May 6 to May 28, 1972 in the Karaginsk Gulf of the Bering Sea. The helminths were collected from 124 specimens of Phoca vitulina largha, 9 specimens of Erignathus barbatus nauticus, 9 specimens of Pusa hispida krascheninnikovi and 9 specimens of Histriophoca fasciata. In all, 23 species, 2 immature and 1 larval form of helminths were recorded. The data are given on the localization of helminths, intensity and extensity of their invasion, pathogenic effect on the host. The age dynamics of the helminths from Phoca vitulina largha was studied. For the first time are reported the trematode Orthosplanchnus pygmaeus, the cestode Diplogonoporus tetrapterus and nematodes Aniskis simplex, Anisakis sp. and Parafilaroides krascheninnikovi in Phoca vitulina largha and the nematodes Anisakis sp. and Phocascaris cystophorae in Pusa hispida krascheninnikovi.", "PMID": 958725}
{"id": "pubmed23n0032_3219", "title": "Inhibition by lymphoid cellular factors of the immunological cytotoxicity against a syngeneic tumour.", "content": "Lymphoid cells of C57BL mice bearing a syngeneic sarcoma are cytotoxic against the same tumour cells in vitro at two different periods of the growth of the tumour separated by a \"mute period\". Without additional antigenic stimulation the lymphoid cells from the \"mute period\" can be made cytotoxic by preincubation in vitro and elimination of adherent cells by a passage through a glass bead column. Either of these treatments alone is inefficient. The cytotoxicity revealed in this way is due to cells with different properties than those that are directly cytotoxic during the 4th week of the tumour growth. The significance of these results is discussed. It is suggested that some other cells have to be eliminated because they compete with or inhibit the activity of the \"mute period\" lymphoid cells made cytotoxic by preincubation.", "contents": "Inhibition by lymphoid cellular factors of the immunological cytotoxicity against a syngeneic tumour. Lymphoid cells of C57BL mice bearing a syngeneic sarcoma are cytotoxic against the same tumour cells in vitro at two different periods of the growth of the tumour separated by a \"mute period\". Without additional antigenic stimulation the lymphoid cells from the \"mute period\" can be made cytotoxic by preincubation in vitro and elimination of adherent cells by a passage through a glass bead column. Either of these treatments alone is inefficient. The cytotoxicity revealed in this way is due to cells with different properties than those that are directly cytotoxic during the 4th week of the tumour growth. The significance of these results is discussed. It is suggested that some other cells have to be eliminated because they compete with or inhibit the activity of the \"mute period\" lymphoid cells made cytotoxic by preincubation.", "PMID": 958732}
{"id": "pubmed23n0032_3220", "title": "Tritiated thymidine uptake by glomerular cells in proliferative glomerulonephritis of the rat.", "content": "A small but significant number of tritiated thymidine labelled cells were found, by autoradiography, in the glomeruli of rats with Masugi nephritis or chronic serum sickness nephritis. There were no labelled glomerular cells in sections of untreated animals. The findings favour the contention that in proliferative glomerulonephritis, glomerular hypercellularity is due to infiltration of monocytic cells into the tufts where they divide.", "contents": "Tritiated thymidine uptake by glomerular cells in proliferative glomerulonephritis of the rat. A small but significant number of tritiated thymidine labelled cells were found, by autoradiography, in the glomeruli of rats with Masugi nephritis or chronic serum sickness nephritis. There were no labelled glomerular cells in sections of untreated animals. The findings favour the contention that in proliferative glomerulonephritis, glomerular hypercellularity is due to infiltration of monocytic cells into the tufts where they divide.", "PMID": 958733}
{"id": "pubmed23n0032_3221", "title": "[Effect of certain factors on block formation in Ceratophillus tesquorum and Neopsylla setosa setosa].", "content": "Studies were conducted of the effect of temperature and humidity, variant of plague microbe, frequency and duration of feeding and specificity of the host on the blockformation in the souslik fleas C. tesquorum and N. setosa infected with plague. 28 tests on the effect of temperature and humidity on the blockformation were undertaken, for which 14411 fleas of the above species were used. A temperature of 16 to 22 degrees proved to be optimal; at this temperature the number of blocked fleas (C. tesquorum) varied from 21.2 to 42.7% and that of N. setosa--from 41.9 to 54.2%. Marmot variant of plague microbe caused the formation of the \"block\" in 53.3 to 55.1% of fleas of N. setosa in 3-4 days and in 28.0 to 42.7% of C. tesquorum in 10-14 days after the infection. In C. tesquorum the process of blockformation is affected by the frequency of feeding, in N. setosa--by the duration of each feeding.", "contents": "[Effect of certain factors on block formation in Ceratophillus tesquorum and Neopsylla setosa setosa]. Studies were conducted of the effect of temperature and humidity, variant of plague microbe, frequency and duration of feeding and specificity of the host on the blockformation in the souslik fleas C. tesquorum and N. setosa infected with plague. 28 tests on the effect of temperature and humidity on the blockformation were undertaken, for which 14411 fleas of the above species were used. A temperature of 16 to 22 degrees proved to be optimal; at this temperature the number of blocked fleas (C. tesquorum) varied from 21.2 to 42.7% and that of N. setosa--from 41.9 to 54.2%. Marmot variant of plague microbe caused the formation of the \"block\" in 53.3 to 55.1% of fleas of N. setosa in 3-4 days and in 28.0 to 42.7% of C. tesquorum in 10-14 days after the infection. In C. tesquorum the process of blockformation is affected by the frequency of feeding, in N. setosa--by the duration of each feeding.", "PMID": 958724}
{"id": "pubmed23n0032_3222", "title": "The normal squamous epithelium of the human uterine cervix: a histological study.", "content": "The normal squamous epithelium of the uterine cervix was investigated in 50 foetuses and newborns, as well as in 207 adult women operated upon because of various benign conditions of the uterus. The epithelium was divided according to the presence or the absence of prominent epithelial papillae. Epithelial papillae occurred in 32% of the cervices in the paediatric age and in 17% in adult women. They were present in the whole squamous epithelium covering the portio vaginalis in all pediatric patients. In 49% of the adult patients epithelial papillae covered the whole portio and/or the transitional zone; in the remaining 51% only a reduced area presented epithelial papillae. Micrometric determinations demonstrated that normal epithelium with papillar formation was thicker than epithelium with smooth epithelial border. This was most prominent in the paediatric patients. The possibility that oestrogenic stimulation (in paediatric patients) and oestrogenic stimulation as well as chronic stromal inflammation (in adult patients) could account for the formation of prominent epithelial papillae was discussed.", "contents": "The normal squamous epithelium of the human uterine cervix: a histological study. The normal squamous epithelium of the uterine cervix was investigated in 50 foetuses and newborns, as well as in 207 adult women operated upon because of various benign conditions of the uterus. The epithelium was divided according to the presence or the absence of prominent epithelial papillae. Epithelial papillae occurred in 32% of the cervices in the paediatric age and in 17% in adult women. They were present in the whole squamous epithelium covering the portio vaginalis in all pediatric patients. In 49% of the adult patients epithelial papillae covered the whole portio and/or the transitional zone; in the remaining 51% only a reduced area presented epithelial papillae. Micrometric determinations demonstrated that normal epithelium with papillar formation was thicker than epithelium with smooth epithelial border. This was most prominent in the paediatric patients. The possibility that oestrogenic stimulation (in paediatric patients) and oestrogenic stimulation as well as chronic stromal inflammation (in adult patients) could account for the formation of prominent epithelial papillae was discussed.", "PMID": 958734}
{"id": "pubmed23n0032_3223", "title": "[Distribution and biology of Apatemon annuligerum (V. Nordmann, 1832) Odening, 1970 (Trematoda, Strigeidida)].", "content": "In Lake Itkul situated north-westwards of the town of Abakan there were found encysted metacercariae of Apatemon annuligerum in the eyes of the perch. Adult fishes are 100% infected, the infection intensity amounting to 28 to 455 cysts (125 on the average). Maximum infection of the perch takes place at the age of 4 to 5 years. In elder fishes proceeds a decrease of infection intensity on account of a considerable mortality of cysts. There was undertaken an infection of incubative ducklings and observed a development of marita to a mature state. The maturation of eggs takes place on the 5-th day.", "contents": "[Distribution and biology of Apatemon annuligerum (V. Nordmann, 1832) Odening, 1970 (Trematoda, Strigeidida)]. In Lake Itkul situated north-westwards of the town of Abakan there were found encysted metacercariae of Apatemon annuligerum in the eyes of the perch. Adult fishes are 100% infected, the infection intensity amounting to 28 to 455 cysts (125 on the average). Maximum infection of the perch takes place at the age of 4 to 5 years. In elder fishes proceeds a decrease of infection intensity on account of a considerable mortality of cysts. There was undertaken an infection of incubative ducklings and observed a development of marita to a mature state. The maturation of eggs takes place on the 5-th day.", "PMID": 958729}
{"id": "pubmed23n0032_3224", "title": "The role of pathology in prenatal diagnosis.", "content": "Antenatal diagnosis of hereditary disease is highly dependent on sufficient theoretical knowledge and on a number of practical methods of studying the foetus such as obtaining, cultivating and assaying amniotic fluid cells. Knowledge of the primary defect in any monogenic disorder cannon be used in prenatal diagnosis unless the metabolic error is expressed in vitro. Modern cytogenetics can diagnose in utero a large majority of karyotyping abnormalities although the karyotype-phenotype correlation is not an absolute one. This task must be assigned to special laboratories where technical pitfalls are reliably avoided. In both metabolic and chromosomal hereditary disease, the pathologist can confirm and extend the phenotypic findings and improve knowledge on foetal features and physiopathology. Pathology is the more important, the less means of in utero diagnosis are available as in the non-chromosomal syndromes of localized or multiple malformations. Here it helps eliminating a present major drawback of prenatal diagnosis: the lack of a strict diagnosis in the previous patient in a family at risk.", "contents": "The role of pathology in prenatal diagnosis. Antenatal diagnosis of hereditary disease is highly dependent on sufficient theoretical knowledge and on a number of practical methods of studying the foetus such as obtaining, cultivating and assaying amniotic fluid cells. Knowledge of the primary defect in any monogenic disorder cannon be used in prenatal diagnosis unless the metabolic error is expressed in vitro. Modern cytogenetics can diagnose in utero a large majority of karyotyping abnormalities although the karyotype-phenotype correlation is not an absolute one. This task must be assigned to special laboratories where technical pitfalls are reliably avoided. In both metabolic and chromosomal hereditary disease, the pathologist can confirm and extend the phenotypic findings and improve knowledge on foetal features and physiopathology. Pathology is the more important, the less means of in utero diagnosis are available as in the non-chromosomal syndromes of localized or multiple malformations. Here it helps eliminating a present major drawback of prenatal diagnosis: the lack of a strict diagnosis in the previous patient in a family at risk.", "PMID": 958735}
{"id": "pubmed23n0032_3225", "title": "[Metacercariae of the genus Diplostomum in the fauna of the USSR].", "content": "The paper deals with the specific composition of the genus diplostomum, parasitic in fishes and Cyclostomata of the USSR. A key is given for the identification of 13 species of the genus. Each species is provided with a brief morphological characteristic including main taxonomic characters of the species.", "contents": "[Metacercariae of the genus Diplostomum in the fauna of the USSR]. The paper deals with the specific composition of the genus diplostomum, parasitic in fishes and Cyclostomata of the USSR. A key is given for the identification of 13 species of the genus. Each species is provided with a brief morphological characteristic including main taxonomic characters of the species.", "PMID": 958726}
{"id": "pubmed23n0032_3226", "title": "Chronic otitis media in children.", "content": "There are two main types of chronic otitis media in children. The \"benign\" type with a central tympanic membrane perforation may heal spontaneously with conservative management. However, if the central tympanic membrane perforation fails to heal, it should be closed surgically--preferably at the age when the incidence of ear disease in children is lowest. Chronic otitis media with cholesteatoma usually has an attic or marginal perforation and requires surgical treatment to prevent serious complications. The incidence of this disease has been decreasing because of awareness by primary physicians of the importance of prompt treatment and the increase in the practice of inserting ventilation tubes.", "contents": "Chronic otitis media in children. There are two main types of chronic otitis media in children. The \"benign\" type with a central tympanic membrane perforation may heal spontaneously with conservative management. However, if the central tympanic membrane perforation fails to heal, it should be closed surgically--preferably at the age when the incidence of ear disease in children is lowest. Chronic otitis media with cholesteatoma usually has an attic or marginal perforation and requires surgical treatment to prevent serious complications. The incidence of this disease has been decreasing because of awareness by primary physicians of the importance of prompt treatment and the increase in the practice of inserting ventilation tubes.", "PMID": 958737}
{"id": "pubmed23n0032_3227", "title": "Vertigo in children.", "content": "The physician should realize that children can have organic vestibular disorders. Therefore, he should seriously approach every child who complains of dizziness, taking a careful history and performing appropriate examinations in order to afford the best possibility of prompt diagnosis and effective treatment.", "contents": "Vertigo in children. The physician should realize that children can have organic vestibular disorders. Therefore, he should seriously approach every child who complains of dizziness, taking a careful history and performing appropriate examinations in order to afford the best possibility of prompt diagnosis and effective treatment.", "PMID": 958738}
{"id": "pubmed23n0032_3228", "title": "[Polymorphism of the median crochets of Dactylogyrus vastator Nyb., 1924 (Monogenoidea) from the crucian carp, Carassius carassius L].", "content": "There are some variations in the dimensions of anchors depending on the age of the host, localisation and number of parasites. To differentiate such close morphological species as D. vastator and D. crassus it has been suggested to take into account, in addition to taxonomic features, the correlation between the anchor ventroapical length and the bend.", "contents": "[Polymorphism of the median crochets of Dactylogyrus vastator Nyb., 1924 (Monogenoidea) from the crucian carp, Carassius carassius L]. There are some variations in the dimensions of anchors depending on the age of the host, localisation and number of parasites. To differentiate such close morphological species as D. vastator and D. crassus it has been suggested to take into account, in addition to taxonomic features, the correlation between the anchor ventroapical length and the bend.", "PMID": 958727}
{"id": "pubmed23n0032_3229", "title": "[New representatives of the lower Monogenea from the gills of fishes of the fam. Fomacentridae from the Gulf of Mexico].", "content": "6 new species of the genus Neohaliotrema Yamaguti, 1965 are described and a new genus and species, Paraneohaliotrema microspathodoni Zhukov gen. et sp. n., (Monogenoidea, Polyonchoinea) from the fishes of the family Pomacentridae from the of Gulf Mexico are erected. the specimens described were found in Abudefduf saxatilis, A. taurus and Microspathodon chrysurus caught in the Habana region. The interrelationships between investigated hosts and their parasites are discussed. The Indo-Westpacific origin of the genus Neohaliotrema has been established.", "contents": "[New representatives of the lower Monogenea from the gills of fishes of the fam. Fomacentridae from the Gulf of Mexico]. 6 new species of the genus Neohaliotrema Yamaguti, 1965 are described and a new genus and species, Paraneohaliotrema microspathodoni Zhukov gen. et sp. n., (Monogenoidea, Polyonchoinea) from the fishes of the family Pomacentridae from the of Gulf Mexico are erected. the specimens described were found in Abudefduf saxatilis, A. taurus and Microspathodon chrysurus caught in the Habana region. The interrelationships between investigated hosts and their parasites are discussed. The Indo-Westpacific origin of the genus Neohaliotrema has been established.", "PMID": 958728}
{"id": "pubmed23n0032_3230", "title": "Nasal problems in children.", "content": "Nasal problems in children are very common. The factors that affect the embryologic development have been discussed. Injuries that occur in prenatal, natal, and postnatal periods affect normal development. Prompt treatment of minor injuries is necessary to prevent airway problems later. The \"wait and see\" attitude toward nasal deformity is ill advised. X-ray findings are not conclusive, as the nasal pyramid in a child is largely cartilaginous. Obstructive nasal breathing can result in facial asymmetry, malocclusion, and cardiopulmonary problems. Allergy and sinusitis are frequently causes of obstruction.", "contents": "Nasal problems in children. Nasal problems in children are very common. The factors that affect the embryologic development have been discussed. Injuries that occur in prenatal, natal, and postnatal periods affect normal development. Prompt treatment of minor injuries is necessary to prevent airway problems later. The \"wait and see\" attitude toward nasal deformity is ill advised. X-ray findings are not conclusive, as the nasal pyramid in a child is largely cartilaginous. Obstructive nasal breathing can result in facial asymmetry, malocclusion, and cardiopulmonary problems. Allergy and sinusitis are frequently causes of obstruction.", "PMID": 958739}
{"id": "pubmed23n0032_3231", "title": "Neck masses in children: diagnosis and treatment.", "content": "Neck masses in children most often represent benign lymphadenitis due to infection. The involved lymph nodes are usually small, shotty, diffusely distributed, and superficial. They may be tender and associated with an acute upper respiratory infection or with chronic infection of the tonsils and adenoids. Enlargement of the jugulodigastric node is most often associated with tonsillitis, and the spinal accessory group of nodes with adenoiditis. Acute viral diseases may be associated with lymphadenopathy; mononucleosis is the most striking example. The differential diagnosis must include deep neck-space abscesses, congenital cysts, and benign as well as malignant neoplasms. A diagnosis can be derived from a consideration of the history and physical findings, the age of the patient, and the location, size, and consistency of the mass. A careful otolaryngologic exam--including the nasopharynx, a chest radiograph, and appropriate blood studies--should be routine. When malignancy is suspected, a biopsy of the mass is indicated.", "contents": "Neck masses in children: diagnosis and treatment. Neck masses in children most often represent benign lymphadenitis due to infection. The involved lymph nodes are usually small, shotty, diffusely distributed, and superficial. They may be tender and associated with an acute upper respiratory infection or with chronic infection of the tonsils and adenoids. Enlargement of the jugulodigastric node is most often associated with tonsillitis, and the spinal accessory group of nodes with adenoiditis. Acute viral diseases may be associated with lymphadenopathy; mononucleosis is the most striking example. The differential diagnosis must include deep neck-space abscesses, congenital cysts, and benign as well as malignant neoplasms. A diagnosis can be derived from a consideration of the history and physical findings, the age of the patient, and the location, size, and consistency of the mass. A careful otolaryngologic exam--including the nasopharynx, a chest radiograph, and appropriate blood studies--should be routine. When malignancy is suspected, a biopsy of the mass is indicated.", "PMID": 958741}
{"id": "pubmed23n0032_3232", "title": "Megavitamin responsive aminoacidopathies.", "content": "Several different vitamins, primarily members of the B complex, serve as coenzymes which activate apoenzymes to produce active holoenzymes. These cofactors participate in reactions in various ways. Even after a specific enzyme diagnosis is made in a patient, it is difficult, if not sometimes impossible, to determine if the patient will respond to a specific cofactor in vivo. The safety and ease of administering these compounds demand that therapeutic trials be undertaken. Doses (see Table 1) of several hundred to several thousand times the normal daily requirements must be given to the patient parenterally or by mouth. The studies in patients with B6-responsive disorders and with maple syrup urine disease would indicate that long term trials of these agents should be given even if there is no short term response. When efficacious, these compounds greatly simplify therapy for patients with inborn errors of amino acid metabolism.", "contents": "Megavitamin responsive aminoacidopathies. Several different vitamins, primarily members of the B complex, serve as coenzymes which activate apoenzymes to produce active holoenzymes. These cofactors participate in reactions in various ways. Even after a specific enzyme diagnosis is made in a patient, it is difficult, if not sometimes impossible, to determine if the patient will respond to a specific cofactor in vivo. The safety and ease of administering these compounds demand that therapeutic trials be undertaken. Doses (see Table 1) of several hundred to several thousand times the normal daily requirements must be given to the patient parenterally or by mouth. The studies in patients with B6-responsive disorders and with maple syrup urine disease would indicate that long term trials of these agents should be given even if there is no short term response. When efficacious, these compounds greatly simplify therapy for patients with inborn errors of amino acid metabolism.", "PMID": 958746}
{"id": "pubmed23n0032_3233", "title": "Hypernatremia--problems in management.", "content": "Hypernatremia may be produced under several different circumstances but most frequently is the result of excessive water loss with diarrhea and the excessive solute load secondary to inappropriate preparation of formula. The clinical manifestations vary and depend primarily on the degree of dehydration and the rate at which the hyperosmolar state has been reached. The management of the patient will, of course, depend upon the mechanism of hypernatremia and degree of dehydration and/or hypovolemia that is present. It seems clear that the exact nature of the rehydrating solution is not of major importance. The volume is of great concern but most vital seems to be the rate of rehydration. If rehydration is accomplished too rapidly the child becomes edematous, develops increased intracranial pressure, stupor, and convulsions. If fluids are given slowly and at a well regulated rate, these complications can usually be avoided. The patient should be monitored regularly with electrolytes, careful determination of weights, and records of intake and output. The rate of rehydration should be monitored to assure that the planned schedule of 24 to 72 hours (depending on the severity of the problem) is followed. Approximately 10 to 15 per cent of children with serum sodium of 160 mEq per liter or greater will have permanent neurological deficits.", "contents": "Hypernatremia--problems in management. Hypernatremia may be produced under several different circumstances but most frequently is the result of excessive water loss with diarrhea and the excessive solute load secondary to inappropriate preparation of formula. The clinical manifestations vary and depend primarily on the degree of dehydration and the rate at which the hyperosmolar state has been reached. The management of the patient will, of course, depend upon the mechanism of hypernatremia and degree of dehydration and/or hypovolemia that is present. It seems clear that the exact nature of the rehydrating solution is not of major importance. The volume is of great concern but most vital seems to be the rate of rehydration. If rehydration is accomplished too rapidly the child becomes edematous, develops increased intracranial pressure, stupor, and convulsions. If fluids are given slowly and at a well regulated rate, these complications can usually be avoided. The patient should be monitored regularly with electrolytes, careful determination of weights, and records of intake and output. The rate of rehydration should be monitored to assure that the planned schedule of 24 to 72 hours (depending on the severity of the problem) is followed. Approximately 10 to 15 per cent of children with serum sodium of 160 mEq per liter or greater will have permanent neurological deficits.", "PMID": 958747}
{"id": "pubmed23n0032_3234", "title": "Carbon monoxide diffusing capacity in newborn infants.", "content": "In order to test the role of diffusing capacity in determining the arterial oxygen tension of newborn infants, pulmonary diffusing capacity for carbonmonoxide (DLCO) was measured in 21 healthy infants ranging in weight from 765 to 4,720 g. DLCO infants without respiratory distress correlated well with lung volume (r=0.76, P less than 0.001). A smaller correlation (r = 0.39, P less than 0.01) was obtained between DLCO and arterial oxygen tension. DLCO per unit volume of lung is similar when healthy premature infants, full term infants, and normal adults are compared. The wide range of normal values obtained in resting infants and the lack of correlation with arterial oxygen tensions are similar to observations made in adults.", "contents": "Carbon monoxide diffusing capacity in newborn infants. In order to test the role of diffusing capacity in determining the arterial oxygen tension of newborn infants, pulmonary diffusing capacity for carbonmonoxide (DLCO) was measured in 21 healthy infants ranging in weight from 765 to 4,720 g. DLCO infants without respiratory distress correlated well with lung volume (r=0.76, P less than 0.001). A smaller correlation (r = 0.39, P less than 0.01) was obtained between DLCO and arterial oxygen tension. DLCO per unit volume of lung is similar when healthy premature infants, full term infants, and normal adults are compared. The wide range of normal values obtained in resting infants and the lack of correlation with arterial oxygen tensions are similar to observations made in adults.", "PMID": 958749}
{"id": "pubmed23n0032_3235", "title": "Skin reflectance in the newborn infant.", "content": "Skin reflectance in red, green, and blue light was measured at the sternum of 99 Caucasian infants ranging in gestational age from 26 to 44 weeks. Skin reflectance was consistently higher in female infants, but this difference was not statistically significant. Highly significant (P less than 0.001) increases in reflectance at all wave lengths were found when sternal reflectance during the first 48 hr of life was related to gestational age. The dispersion of data points about the regression line does not permit this method to be relied upon as the sole means of determining gestational age of infants. Serial studies indicate that reflectance increases in premature infants not given phototherapy, whereas premature infants receiving phototherapy show a fall in reflectance for the duration of therapy. This suggests that phototherapy may cause tanning of the skin.", "contents": "Skin reflectance in the newborn infant. Skin reflectance in red, green, and blue light was measured at the sternum of 99 Caucasian infants ranging in gestational age from 26 to 44 weeks. Skin reflectance was consistently higher in female infants, but this difference was not statistically significant. Highly significant (P less than 0.001) increases in reflectance at all wave lengths were found when sternal reflectance during the first 48 hr of life was related to gestational age. The dispersion of data points about the regression line does not permit this method to be relied upon as the sole means of determining gestational age of infants. Serial studies indicate that reflectance increases in premature infants not given phototherapy, whereas premature infants receiving phototherapy show a fall in reflectance for the duration of therapy. This suggests that phototherapy may cause tanning of the skin.", "PMID": 958750}
{"id": "pubmed23n0032_3236", "title": "The effect of promethazine hydrochloride on bilirubin metabolism in the rat.", "content": "The effect of 21 days of promethazine-HC1 administration on hepatic bilirubin metabolism and transport was studied in adult rats. A significant increase in mean cumulative hepatic bilirubin uptake (84.5 +/- 7.6 (SE) mug/100 g/min in controls vs. 110.0 +/- 4.3 in treated rats), mean hepatic glucuronide conjugation (1,330 +/- 86 (SE) mug bilirubin conjugated/g liver/40 min in controls vs. 1.713 +/- 61 in treated rats), and mean maximal hepatic excretion (47.2 +/- 4.9 (SE) mug/100 g/min vs. 63.5 +/- 2.7) was observed in treated animals. Mean total liver weight and total hepatic protein also increased significantly. These observations suggest that promethazine is an inducer of protein and enzyme synthesis in rat liver and is capable of significantly stimulating the three major steps in hepatic disposal of bilirubin.", "contents": "The effect of promethazine hydrochloride on bilirubin metabolism in the rat. The effect of 21 days of promethazine-HC1 administration on hepatic bilirubin metabolism and transport was studied in adult rats. A significant increase in mean cumulative hepatic bilirubin uptake (84.5 +/- 7.6 (SE) mug/100 g/min in controls vs. 110.0 +/- 4.3 in treated rats), mean hepatic glucuronide conjugation (1,330 +/- 86 (SE) mug bilirubin conjugated/g liver/40 min in controls vs. 1.713 +/- 61 in treated rats), and mean maximal hepatic excretion (47.2 +/- 4.9 (SE) mug/100 g/min vs. 63.5 +/- 2.7) was observed in treated animals. Mean total liver weight and total hepatic protein also increased significantly. These observations suggest that promethazine is an inducer of protein and enzyme synthesis in rat liver and is capable of significantly stimulating the three major steps in hepatic disposal of bilirubin.", "PMID": 958751}
{"id": "pubmed23n0032_3237", "title": "Effect of hypoxia on erythroblasts from avian fetal liver: adenosine triphosphate levels and hemoglobin synthesis.", "content": "A system for the isolation and functional evaluation of fetal liver erythroblasts is described. Isolated erythroblasts were prepared from 14-day embryonic avian livers and incubated at various oxygen tension (0, 5, 12, and 95%). The concentration of ATP in erythroblasts remained constant for at least 4 hr at 37degree, but was rapidly reduced by incubation in nitrogen. Protein synthesis as measured by L-[14C]leucine incorporation into cell protein occurred at a linear rate in 5%, 12%, and 95% oxygen, whereas little protein synthesis occurred at 0% oxygen. The effect of hypoxia on the type of hemoglobin synthesized was studied in this system by isolating the hemoglobin A, hemoglobin D, and hemoglobin H fractions and determining the incorporation of L-[14C]leucine. The major fraction, hemoglobin A, contained most of the radioactivity; smaller amounts were present in hemoglobin D and hemoglobin H, respectively. The relative proportion of each hemoglobin synthesized was not altered by oxygen from 5% to 95%. These results argue against a direct effect of oxygen on the type of hemoglobin synthesized at this stage of development.", "contents": "Effect of hypoxia on erythroblasts from avian fetal liver: adenosine triphosphate levels and hemoglobin synthesis. A system for the isolation and functional evaluation of fetal liver erythroblasts is described. Isolated erythroblasts were prepared from 14-day embryonic avian livers and incubated at various oxygen tension (0, 5, 12, and 95%). The concentration of ATP in erythroblasts remained constant for at least 4 hr at 37degree, but was rapidly reduced by incubation in nitrogen. Protein synthesis as measured by L-[14C]leucine incorporation into cell protein occurred at a linear rate in 5%, 12%, and 95% oxygen, whereas little protein synthesis occurred at 0% oxygen. The effect of hypoxia on the type of hemoglobin synthesized was studied in this system by isolating the hemoglobin A, hemoglobin D, and hemoglobin H fractions and determining the incorporation of L-[14C]leucine. The major fraction, hemoglobin A, contained most of the radioactivity; smaller amounts were present in hemoglobin D and hemoglobin H, respectively. The relative proportion of each hemoglobin synthesized was not altered by oxygen from 5% to 95%. These results argue against a direct effect of oxygen on the type of hemoglobin synthesized at this stage of development.", "PMID": 958752}
{"id": "pubmed23n0032_3238", "title": "Action of growth hormone on erythropoiesis: changes in red blood cell enzyme activities in growth-retarded patients with and without growth hormone deficiency.", "content": "Fifteen red cell enzyme activities of growth-retarded patients with and without growth hormone (GH) deficiency were investigated before and after GH administration. The 15 enzymes were Hexokinase, phosphoglucomutase, glucose phosphate, isomerase, phosphofructokinase, fructose diphosphate aldolase, glyceraldehyde-3-phosphae dehydrogenase, triosephosphate isomerase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase, 3-phosphoglycerate mutase, enolase, pyruvate kinase, glycose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, glutathione reducase. Sixty-six subjects were studied: 30 normal control subjects (group N) and 36 patients (aged 5-23 years) with short stature. Complete endocrine evaluation showed 21 (group I) to have GH deficiency (10 patients with isolated GH deficiency) and 15 (group II) to have normal hypothalamic and pituitary function except for two patients with a moderate hypothyroidism. Both had been receiving thyroid hormone treatment for a long time before our studies. All 36 patients were treated with 2 mg human growth hormone intramuscularly for 7 days. Before GH treatment no significant difference was observed between hematologic data in group I (GH deficiency) and group II (no GH deficiency). After GH therapy there was a significant increase in reticulocyte count in both groups of patients with short stature. The mean pretreatment value in group I was 1.294% +/- 0.084 (SEM); the mean post-treatment value was 2.081% +/- 0.287 (SEM)< P less than 0.005. The mean pretreatment value in group II was 1.0% 0.184 (SEM); the mean post-treatment value was 1.407% +/- 0.193 (SEM), P less than 0.01. In group II (no GH deficiency) mean pretreatment erythrocyte enzyme activities were not significantly different from those activities observed in normal control subjects (group N). However, in patients who lacked GH, the pretreatment activities of five red cell enzymes (glucose phosphate isomerase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase) were significantly decreased before GH administration compared with the values in normal control subjects...", "contents": "Action of growth hormone on erythropoiesis: changes in red blood cell enzyme activities in growth-retarded patients with and without growth hormone deficiency. Fifteen red cell enzyme activities of growth-retarded patients with and without growth hormone (GH) deficiency were investigated before and after GH administration. The 15 enzymes were Hexokinase, phosphoglucomutase, glucose phosphate, isomerase, phosphofructokinase, fructose diphosphate aldolase, glyceraldehyde-3-phosphae dehydrogenase, triosephosphate isomerase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase, 3-phosphoglycerate mutase, enolase, pyruvate kinase, glycose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, glutathione reducase. Sixty-six subjects were studied: 30 normal control subjects (group N) and 36 patients (aged 5-23 years) with short stature. Complete endocrine evaluation showed 21 (group I) to have GH deficiency (10 patients with isolated GH deficiency) and 15 (group II) to have normal hypothalamic and pituitary function except for two patients with a moderate hypothyroidism. Both had been receiving thyroid hormone treatment for a long time before our studies. All 36 patients were treated with 2 mg human growth hormone intramuscularly for 7 days. Before GH treatment no significant difference was observed between hematologic data in group I (GH deficiency) and group II (no GH deficiency). After GH therapy there was a significant increase in reticulocyte count in both groups of patients with short stature. The mean pretreatment value in group I was 1.294% +/- 0.084 (SEM); the mean post-treatment value was 2.081% +/- 0.287 (SEM)< P less than 0.005. The mean pretreatment value in group II was 1.0% 0.184 (SEM); the mean post-treatment value was 1.407% +/- 0.193 (SEM), P less than 0.01. In group II (no GH deficiency) mean pretreatment erythrocyte enzyme activities were not significantly different from those activities observed in normal control subjects (group N). However, in patients who lacked GH, the pretreatment activities of five red cell enzymes (glucose phosphate isomerase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase) were significantly decreased before GH administration compared with the values in normal control subjects...", "PMID": 958753}
{"id": "pubmed23n0032_3239", "title": "Nutritionally small-for-dates rats: their subsequent growth, regional brain 5-hydroxytryptamine turnover, and behavior.", "content": "Rats were subjected to nutritional growth retardation either from conception to 5 postnatal days of age (fetal and neonatal restriction (FNR) group), or from 5 to 25 postnatal days of age (infantile restriction (IR) group). The FNR group may serve as a model for the human small-for-dates baby. At 20 weeks of age cerebellum, midbrain, and cerebrum were significantly reduced in weight by 4%, 5%, and 4%, respectively, in FNR animals when compared with controls. Only cerebellum and midbrain were affected in IR rats of the same age, but in both regions the percentage deficits (8% and 9%, respectively) were greater than in FNR animals. Both cerebellum and midbrain weighed significantly less in IR than in FNR rats. The timing of nutritional growth retardation appeared to be of little consequence to the regional brain turnover of 5-hydroxytryptamine in adulthood. The rate of synthesis in the hippocampus of both FNR and IR animals was significantly faster (67% and 75% respectively) than in controls. The increased turnover could perhaps represent \"overactivity\" of those 5-hydroxytryptaminergic neurons terminating in the hippocampus. Some differences in the behavior of the previously undernourished adult animals were also evident. On the fifth day of testing, control rats were most venturesome in the open field. Eighteen control rats left the edge zone within 2 min, whereas only 8 FNR and 11 IR rats did so. Most animals froze immediately after a 7-sex exposure to a loud electric bell. The delay before moving about again differentiated the three groups. FNR rats took longest to move out of the area in which they froze.", "contents": "Nutritionally small-for-dates rats: their subsequent growth, regional brain 5-hydroxytryptamine turnover, and behavior. Rats were subjected to nutritional growth retardation either from conception to 5 postnatal days of age (fetal and neonatal restriction (FNR) group), or from 5 to 25 postnatal days of age (infantile restriction (IR) group). The FNR group may serve as a model for the human small-for-dates baby. At 20 weeks of age cerebellum, midbrain, and cerebrum were significantly reduced in weight by 4%, 5%, and 4%, respectively, in FNR animals when compared with controls. Only cerebellum and midbrain were affected in IR rats of the same age, but in both regions the percentage deficits (8% and 9%, respectively) were greater than in FNR animals. Both cerebellum and midbrain weighed significantly less in IR than in FNR rats. The timing of nutritional growth retardation appeared to be of little consequence to the regional brain turnover of 5-hydroxytryptamine in adulthood. The rate of synthesis in the hippocampus of both FNR and IR animals was significantly faster (67% and 75% respectively) than in controls. The increased turnover could perhaps represent \"overactivity\" of those 5-hydroxytryptaminergic neurons terminating in the hippocampus. Some differences in the behavior of the previously undernourished adult animals were also evident. On the fifth day of testing, control rats were most venturesome in the open field. Eighteen control rats left the edge zone within 2 min, whereas only 8 FNR and 11 IR rats did so. Most animals froze immediately after a 7-sex exposure to a loud electric bell. The delay before moving about again differentiated the three groups. FNR rats took longest to move out of the area in which they froze.", "PMID": 958754}
{"id": "pubmed23n0032_3240", "title": "Pediatric automotive restraints, pediatricians, and the academy.", "content": "Over 70% of the 192 Southern California Academy pediatricians responding to a survey teach parents about pediatric automotive safety devices but less than 3% do so on every visit. To test two methods of increasing the frequency of teaching, these pediatricians were randomly assigned to either a mailing from the Academy's local chapter (mail group) or a brief presentation by a local pharmaceutical representative at his regular visit (interview group); a follow-up was conducted one month later by mail. Sixty-one percent of the mail group and 49% of the interview group claimed that their teaching on this subject had increased since the original contact. While this difference did not reach statistical significance, it was in the same direction as the preferences for sources of pediatric information expressed by both groups of pediatricians. A special letter from the Academy was ranked most effective and a visit from the pharmaceutical representative was judged least effective. Because of the magnitude of the problem, availability of a solution, proof that pediatricians can influence health behavior related to children and acknowledgment of the Academy's role in pediatric education, an appeal is made for an all-out campaign by the Academy and its members to promote use of appropriate pediatric automotive safety devices.", "contents": "Pediatric automotive restraints, pediatricians, and the academy. Over 70% of the 192 Southern California Academy pediatricians responding to a survey teach parents about pediatric automotive safety devices but less than 3% do so on every visit. To test two methods of increasing the frequency of teaching, these pediatricians were randomly assigned to either a mailing from the Academy's local chapter (mail group) or a brief presentation by a local pharmaceutical representative at his regular visit (interview group); a follow-up was conducted one month later by mail. Sixty-one percent of the mail group and 49% of the interview group claimed that their teaching on this subject had increased since the original contact. While this difference did not reach statistical significance, it was in the same direction as the preferences for sources of pediatric information expressed by both groups of pediatricians. A special letter from the Academy was ranked most effective and a visit from the pharmaceutical representative was judged least effective. Because of the magnitude of the problem, availability of a solution, proof that pediatricians can influence health behavior related to children and acknowledgment of the Academy's role in pediatric education, an appeal is made for an all-out campaign by the Academy and its members to promote use of appropriate pediatric automotive safety devices.", "PMID": 958757}
{"id": "pubmed23n0032_3241", "title": "Car safety for infants: effectiveness of prenatal counseling.", "content": "In a pediatric office setting, car safety counseling was provided to 16 primigravida women during a prenatal pediatric interview. A control group of 19 women received no counseling during a similar interview. Forty-two percent of noncounseled mothers and 69% of counseled mothers were using a safe infant restraint system at the six-week well-child visit. Preventive counseling in car safety is an important role for the pediatrician.", "contents": "Car safety for infants: effectiveness of prenatal counseling. In a pediatric office setting, car safety counseling was provided to 16 primigravida women during a prenatal pediatric interview. A control group of 19 women received no counseling during a similar interview. Forty-two percent of noncounseled mothers and 69% of counseled mothers were using a safe infant restraint system at the six-week well-child visit. Preventive counseling in car safety is an important role for the pediatrician.", "PMID": 958758}
{"id": "pubmed23n0032_3242", "title": "A ten-year assessment of methicillin-associated side effects.", "content": "The exact incidence and importance of side effects associated with methicillin therapy in children is unknown. During the ten-year period from 1964 to 1974 approximately 3,000 Houston children who received methicillin were observed for side effects. The great majority of these patients received the drug for less than ten days; however, side effects were more common in patients receiving long-term therapy. In order to assess these side effects, experiences with 124 children who received methicillin for ten days or longer were reviewed in depth. The average dose of methicillin was 200 mg/kg/day and the average duration was 22 days. In this highly selected group there were no adverse side effects in 54.8% and only eosinophilia in an additional 13%. Leukopenia occurred in 16%, microscopic hematuria in 8%, gross hematuria in 4%, skin rash in 6%, and \"drug fever\" in 6%. In many instances several of these side effects occurred within a single patient so that the total number of patients with definite side effects was 39 of 124 (31.5%). The true incidence figure of overall side effects is much lower than 31.5%. Corrected incidence rates based on a conservative figure of  3,000 methicillin-treated children are less than 1.5% for all side effects. In some patients the mechanism producing the adverse reactions seemed to be chemical irritation or toxicity and in others hypersensitivity. In nine of the 39 patients follow-up studies were not optimal. In the other 30 patients all side effects were reversible.", "contents": "A ten-year assessment of methicillin-associated side effects. The exact incidence and importance of side effects associated with methicillin therapy in children is unknown. During the ten-year period from 1964 to 1974 approximately 3,000 Houston children who received methicillin were observed for side effects. The great majority of these patients received the drug for less than ten days; however, side effects were more common in patients receiving long-term therapy. In order to assess these side effects, experiences with 124 children who received methicillin for ten days or longer were reviewed in depth. The average dose of methicillin was 200 mg/kg/day and the average duration was 22 days. In this highly selected group there were no adverse side effects in 54.8% and only eosinophilia in an additional 13%. Leukopenia occurred in 16%, microscopic hematuria in 8%, gross hematuria in 4%, skin rash in 6%, and \"drug fever\" in 6%. In many instances several of these side effects occurred within a single patient so that the total number of patients with definite side effects was 39 of 124 (31.5%). The true incidence figure of overall side effects is much lower than 31.5%. Corrected incidence rates based on a conservative figure of  3,000 methicillin-treated children are less than 1.5% for all side effects. In some patients the mechanism producing the adverse reactions seemed to be chemical irritation or toxicity and in others hypersensitivity. In nine of the 39 patients follow-up studies were not optimal. In the other 30 patients all side effects were reversible.", "PMID": 958759}
{"id": "pubmed23n0032_3243", "title": "Treatment of apnea of prematurity with aminophylline.", "content": "The effectiveness of aminophylline in the treatment of apnea of prematurity was evaluated in 13 premature infants (mean birthweight, 1.13 kg; mean gestational age, 29 weeks). Apnea was recorded by direct observation in combination with impedance monitoring. Rectal suppositories of aminophylline (5 mg) were given at six-hour intervals. The average dose was 4.1 mg/kg. No toxicity or complications were noted. The parents became free of apneic episodes during therapy. The response for each eight-hour interval of treatment over 72 hours when compared to pretreatment was significant (P less than .01; paired t-test), after the first eight hours. Only one patient required mechanical ventilation for apnea. Treatment was continued for 2 to 14 days (mean, 5 days). A recurrence of apnea was noted in nine patients after discontinuing aminophylline. All patients except one survived. No change in Po2, Pco2, pH, mean heart and respiratory rates, and blood pressure was noted. A direct effect on the respiratory center is postulated.", "contents": "Treatment of apnea of prematurity with aminophylline. The effectiveness of aminophylline in the treatment of apnea of prematurity was evaluated in 13 premature infants (mean birthweight, 1.13 kg; mean gestational age, 29 weeks). Apnea was recorded by direct observation in combination with impedance monitoring. Rectal suppositories of aminophylline (5 mg) were given at six-hour intervals. The average dose was 4.1 mg/kg. No toxicity or complications were noted. The parents became free of apneic episodes during therapy. The response for each eight-hour interval of treatment over 72 hours when compared to pretreatment was significant (P less than .01; paired t-test), after the first eight hours. Only one patient required mechanical ventilation for apnea. Treatment was continued for 2 to 14 days (mean, 5 days). A recurrence of apnea was noted in nine patients after discontinuing aminophylline. All patients except one survived. No change in Po2, Pco2, pH, mean heart and respiratory rates, and blood pressure was noted. A direct effect on the respiratory center is postulated.", "PMID": 958760}
{"id": "pubmed23n0032_3244", "title": "An influenza B epidemic among children in day-care.", "content": "An outbreak of influenza virus type B infections occurred in the Frank Porter Graham Day Care Center from February to April, 1974. During the epidemic there were 27 isolations of influenza B virus from 20 children. One half of these were obtained from children who were well at the time of culturing. Attack rates as determined by virus isolation and seroconversion for most age groups approximated the 40% rate for the entire population. Clinical and microbiological data from this study were compared to those obtained in the center during the same three months in 1973. Increases in the incidence of otitis media and temperatures over 38 C were seen during the 1974 study. Intensive continuous microbiological surveillance for bacteria, viruses, and mycoplasmas failed to reveal predominance of any other potential pathogen to account for these clinical findings. The analysis permitted by the nature of this study design in a day-care setting revealed several unexpected findings: high attack rates unrelated to age; common inapparent infections; and frequent association with otitis media.", "contents": "An influenza B epidemic among children in day-care. An outbreak of influenza virus type B infections occurred in the Frank Porter Graham Day Care Center from February to April, 1974. During the epidemic there were 27 isolations of influenza B virus from 20 children. One half of these were obtained from children who were well at the time of culturing. Attack rates as determined by virus isolation and seroconversion for most age groups approximated the 40% rate for the entire population. Clinical and microbiological data from this study were compared to those obtained in the center during the same three months in 1973. Increases in the incidence of otitis media and temperatures over 38 C were seen during the 1974 study. Intensive continuous microbiological surveillance for bacteria, viruses, and mycoplasmas failed to reveal predominance of any other potential pathogen to account for these clinical findings. The analysis permitted by the nature of this study design in a day-care setting revealed several unexpected findings: high attack rates unrelated to age; common inapparent infections; and frequent association with otitis media.", "PMID": 958761}
{"id": "pubmed23n0032_3245", "title": "A sequential study of various modes of skin and umbilical care and the incidence of staphylococcal colonization and infection in the neonate.", "content": "The rates of colonization and infection of newborns with coagulase-positive staphylococci were studied during five sequential periods using various modes of skin care in the nursery. Colonization and infection were low during the baseline period when total body bathing with 3% hexachlorophene was employed (period 1), but increased dramatically (80% colonization, 9.5% infection) when hexachlorophene was discontinued and replaced by Ivory Soap baths (period 2). Reinstitution of hexachlorophene (period 3) reduced both colonization and infection, but not to the low levels seen during period 1. A second Ivory Soap period (period 4) resulted in a return to high colonization (77%) and infection (11.5%) rates. During period 5, daily Ivory Soap baths were continued and bacitracin ointment was applied to the umbilical area at least three times daily. Colonization was reduced to 10% and infection to 3.0%. Bacitracin could not be detected in serum in 15 infants studied. Colonization with gram-negative enteric bacilli was highest while using hexachlorophene or Ivory-bacitracin, but no increase in gram-negative infections was seen. Colonization of newborns with non-group A beta-hemolytic streptococci was not influenced predictably with various modes of skin care. The local application of bacitracin is a safe and effective method of controlling staphylococcal colonization and disease for infants in nurseries.", "contents": "A sequential study of various modes of skin and umbilical care and the incidence of staphylococcal colonization and infection in the neonate. The rates of colonization and infection of newborns with coagulase-positive staphylococci were studied during five sequential periods using various modes of skin care in the nursery. Colonization and infection were low during the baseline period when total body bathing with 3% hexachlorophene was employed (period 1), but increased dramatically (80% colonization, 9.5% infection) when hexachlorophene was discontinued and replaced by Ivory Soap baths (period 2). Reinstitution of hexachlorophene (period 3) reduced both colonization and infection, but not to the low levels seen during period 1. A second Ivory Soap period (period 4) resulted in a return to high colonization (77%) and infection (11.5%) rates. During period 5, daily Ivory Soap baths were continued and bacitracin ointment was applied to the umbilical area at least three times daily. Colonization was reduced to 10% and infection to 3.0%. Bacitracin could not be detected in serum in 15 infants studied. Colonization with gram-negative enteric bacilli was highest while using hexachlorophene or Ivory-bacitracin, but no increase in gram-negative infections was seen. Colonization of newborns with non-group A beta-hemolytic streptococci was not influenced predictably with various modes of skin care. The local application of bacitracin is a safe and effective method of controlling staphylococcal colonization and disease for infants in nurseries.", "PMID": 958762}
{"id": "pubmed23n0032_3246", "title": "Management of tracheobronchial foreign bodies in children: a reevaluation of postural drainage and bronchoscopy.", "content": "The efficacy of the inhalation-postural drainage technique for removal of aspirated foreign bodies was compared with that of bronchoscopy in 76 children. Twelve of 49 children on postural drainage coughed out the foreign body (25%); the other 37 required bronchoscopy. The foreign body was successfully removed in 56 of 63 children who were bronchoscoped (89%). Our experience suggests that a trial of inhalation-postural drainage, administered in a hospital, may be valuable in the initial management of aspirated foreign bodies. If unsuccessful after several treatments, however, the technique should be abandoned, and bronchoscopy performed. Delay of foreign body removal beyond 24 hours may be associated with increased morbidity and prolonged hospital stay. With recent improvements in pediatric endoscopic instruments, the efficacy of bronchoscopy exceeds 90%.", "contents": "Management of tracheobronchial foreign bodies in children: a reevaluation of postural drainage and bronchoscopy. The efficacy of the inhalation-postural drainage technique for removal of aspirated foreign bodies was compared with that of bronchoscopy in 76 children. Twelve of 49 children on postural drainage coughed out the foreign body (25%); the other 37 required bronchoscopy. The foreign body was successfully removed in 56 of 63 children who were bronchoscoped (89%). Our experience suggests that a trial of inhalation-postural drainage, administered in a hospital, may be valuable in the initial management of aspirated foreign bodies. If unsuccessful after several treatments, however, the technique should be abandoned, and bronchoscopy performed. Delay of foreign body removal beyond 24 hours may be associated with increased morbidity and prolonged hospital stay. With recent improvements in pediatric endoscopic instruments, the efficacy of bronchoscopy exceeds 90%.", "PMID": 958763}
{"id": "pubmed23n0032_3247", "title": "Two programs for predicting adult stature for individuals.", "content": "Two computer programs are described which use the new RWT method to predict adult stature for individuals. This method, developed and described by Roche et al. is the best and most accurate one available to date. The two programs are prepared with different aims in different languages. Program 1 is interactive, written in BASIC and is aimed for the clinician who needs predictions for just a few individuals at a time. Program 2 is written in FORTRAN and is meant for large-scale batch processing. It is thought that this mode is better for the researcher who is more interested in large-scale testing of hypotheses about such things as the efficacy of intervention programs in human growth.", "contents": "Two programs for predicting adult stature for individuals. Two computer programs are described which use the new RWT method to predict adult stature for individuals. This method, developed and described by Roche et al. is the best and most accurate one available to date. The two programs are prepared with different aims in different languages. Program 1 is interactive, written in BASIC and is aimed for the clinician who needs predictions for just a few individuals at a time. Program 2 is written in FORTRAN and is meant for large-scale batch processing. It is thought that this mode is better for the researcher who is more interested in large-scale testing of hypotheses about such things as the efficacy of intervention programs in human growth.", "PMID": 958764}
{"id": "pubmed23n0032_3248", "title": "Effect of chronic salt and water loading on the tubular defects of a child with Fanconi syndrome (cystinosis).", "content": "A male child discovered to have cystinosis and Fanconi syndrome at the age of 2 years 8 months was hospitalized repeatedly for episodes of hyponatremic, hypokalemic dehydration and acidosis. Attempts to correct the fluid and electrolyte abnormalities by increasing progressively the supplements of sodium chloride, sodium bicarbonate, and potassium chloride resulted only in an exacerbation of the symptoms. Tests of discrete renal functions and metabolic balance studies disclosed that, although all disturbances were expressions of the primary disease, their severity was enhanced considerably by the extracellular volume expansion induced by the vigorous therapy. Restriction in water intake resulted in an improvement in the renal performance and in the clinical condition.", "contents": "Effect of chronic salt and water loading on the tubular defects of a child with Fanconi syndrome (cystinosis). A male child discovered to have cystinosis and Fanconi syndrome at the age of 2 years 8 months was hospitalized repeatedly for episodes of hyponatremic, hypokalemic dehydration and acidosis. Attempts to correct the fluid and electrolyte abnormalities by increasing progressively the supplements of sodium chloride, sodium bicarbonate, and potassium chloride resulted only in an exacerbation of the symptoms. Tests of discrete renal functions and metabolic balance studies disclosed that, although all disturbances were expressions of the primary disease, their severity was enhanced considerably by the extracellular volume expansion induced by the vigorous therapy. Restriction in water intake resulted in an improvement in the renal performance and in the clinical condition.", "PMID": 958765}
{"id": "pubmed23n0032_3249", "title": "Growth of hyperkinetic children taking methylphenidate, dextroamphetamine, or imipramine/desipramine.", "content": "One hundred children with the hyperkinetic syndrome or minimal brain dysfunction syndrome were treated with medication: 60 with methylphenidate (Ritalin), 24 with dextroamphetamine (Dexedrine), and 16 with either imipramine or desipramine. The duration of treatment was for a minimum of two years, and averaged five years, with an average follow-up of six years from the onset of treatment. Their weight and height had been measured prior to treatment, and these and subsequent measurements were converted to percentiles, using the tables of norms of the Iowa City study. Initially there was a diminution in expected weight, but not height, but after a few years the growth in weight and height was found to be greater than predicted from the norms to a statistically significant degree. Gains in weight and height were greater for those whose medication had been stopped prior to the final measurements than for those still taking medication; but these differences did not reach statistical significance. No correlations were found between dosage level and changes in weight and height percentiles. It is concluded that there is no stunting of growth from the long-term use of methylphenidate, dextroamphetamine, or imipramine/desipramine in children. Any slowing of growth when treatment is first started is compensated for later on, both while the patient is still taking the medication, and after discontinuing it.", "contents": "Growth of hyperkinetic children taking methylphenidate, dextroamphetamine, or imipramine/desipramine. One hundred children with the hyperkinetic syndrome or minimal brain dysfunction syndrome were treated with medication: 60 with methylphenidate (Ritalin), 24 with dextroamphetamine (Dexedrine), and 16 with either imipramine or desipramine. The duration of treatment was for a minimum of two years, and averaged five years, with an average follow-up of six years from the onset of treatment. Their weight and height had been measured prior to treatment, and these and subsequent measurements were converted to percentiles, using the tables of norms of the Iowa City study. Initially there was a diminution in expected weight, but not height, but after a few years the growth in weight and height was found to be greater than predicted from the norms to a statistically significant degree. Gains in weight and height were greater for those whose medication had been stopped prior to the final measurements than for those still taking medication; but these differences did not reach statistical significance. No correlations were found between dosage level and changes in weight and height percentiles. It is concluded that there is no stunting of growth from the long-term use of methylphenidate, dextroamphetamine, or imipramine/desipramine in children. Any slowing of growth when treatment is first started is compensated for later on, both while the patient is still taking the medication, and after discontinuing it.", "PMID": 958770}
{"id": "pubmed23n0032_3250", "title": "Effect of activated charcoal on acetaminophen absorption.", "content": "Acetaminophen intoxication can cause hepatic, renal, and myocardial necrosis which is often fatal. These lesions develop very rapidly, perhaps during the first pass of the drug through the liver. In case of acute ingestion of an overdose it is therefore essential to employ measures for reducing the absorption of acetaminophen. The effect of activated charcoal on acetaminophen absorption by normal volunteers was determined as a function of the dose of charcoal, the dosage form of acetaminophen, and the charcoal-to-acetaminophen dose ratio. The results indicate that activated charcoal can be an effective antidote for acute acetaminophen intoxication, if administered promptly and in sufficient quantity.", "contents": "Effect of activated charcoal on acetaminophen absorption. Acetaminophen intoxication can cause hepatic, renal, and myocardial necrosis which is often fatal. These lesions develop very rapidly, perhaps during the first pass of the drug through the liver. In case of acute ingestion of an overdose it is therefore essential to employ measures for reducing the absorption of acetaminophen. The effect of activated charcoal on acetaminophen absorption by normal volunteers was determined as a function of the dose of charcoal, the dosage form of acetaminophen, and the charcoal-to-acetaminophen dose ratio. The results indicate that activated charcoal can be an effective antidote for acute acetaminophen intoxication, if administered promptly and in sufficient quantity.", "PMID": 958771}
{"id": "pubmed23n0032_3251", "title": "Childhood hypersensitivity pneumonitis due to dove antigens.", "content": "A case of hypersensitivity pneumonitis due to doves is reported and compared with other cases due to dove or pigeon antigens reported in children. The diagnosis is substantiated by the presence of precipitating antibody to dove and pigeon serum, clinical improvement after contact with the doves was broken, and a positive response to inhalation challenge with pigeon serum. The insidious nature of this disease is emphasized as well as the importance of having detailed environmental information in children with unexplained respiratory disease.", "contents": "Childhood hypersensitivity pneumonitis due to dove antigens. A case of hypersensitivity pneumonitis due to doves is reported and compared with other cases due to dove or pigeon antigens reported in children. The diagnosis is substantiated by the presence of precipitating antibody to dove and pigeon serum, clinical improvement after contact with the doves was broken, and a positive response to inhalation challenge with pigeon serum. The insidious nature of this disease is emphasized as well as the importance of having detailed environmental information in children with unexplained respiratory disease.", "PMID": 958772}
{"id": "pubmed23n0032_3252", "title": "Paraplegia associated with umbilical artery catheterization in the newborn.", "content": "Major complications have followed the use of umbilical artery catheterization in the newborn. The procedure is often a vital part of the monitoring of the critically ill infant and efforts are therefore made to reduce these risks. Recently, we have encountered two newborn infants who developed paraplegia for which no definite etiology could be uncovered. However, their management was attended by the use of umbilical artery catheters and it is postulated that the etiology of paraplegia may have resulted from embolization from these potential thrombogenic sources. The blood supply of the spinal cord and the site of possible embolization are discussed.", "contents": "Paraplegia associated with umbilical artery catheterization in the newborn. Major complications have followed the use of umbilical artery catheterization in the newborn. The procedure is often a vital part of the monitoring of the critically ill infant and efforts are therefore made to reduce these risks. Recently, we have encountered two newborn infants who developed paraplegia for which no definite etiology could be uncovered. However, their management was attended by the use of umbilical artery catheters and it is postulated that the etiology of paraplegia may have resulted from embolization from these potential thrombogenic sources. The blood supply of the spinal cord and the site of possible embolization are discussed.", "PMID": 958773}
{"id": "pubmed23n0032_3253", "title": "Determinants of personal space in young children: perception of distance cues.", "content": "Two methods of assessing personal space in young children were examined in a group of 24 boys and 24 girls, aged 3 to 6 yr. When sex of peer figures and affect attributed to them were varied, 3-yr.-olds made distance-cue judgements based on affect but failed to use affect cues in structuring personal space. However, 4- to 6-yr.-olds readily perceived and used distance to communicate the affective quality of peer relations. On both measures, perception of distance cues increased with age. Only girls also used sex of figures as a social cue in depicting appropriate distances.", "contents": "Determinants of personal space in young children: perception of distance cues. Two methods of assessing personal space in young children were examined in a group of 24 boys and 24 girls, aged 3 to 6 yr. When sex of peer figures and affect attributed to them were varied, 3-yr.-olds made distance-cue judgements based on affect but failed to use affect cues in structuring personal space. However, 4- to 6-yr.-olds readily perceived and used distance to communicate the affective quality of peer relations. On both measures, perception of distance cues increased with age. Only girls also used sex of figures as a social cue in depicting appropriate distances.", "PMID": 958801}
{"id": "pubmed23n0032_3254", "title": "Are two hands better than one? assessing information acquired from one- and two-handed haptic exploration of random forms.", "content": "15 male undergraduates were presented with 10-sided random forms which they explored visually, or one-handed haptically, or two-handed haptically. Forms were exposed for various durations and subjects were required to draw each form immediately after its removal. The results indicated that 30 sec. of one-handed exploration and 20 and 30 sec. of two-handed exploration produced performance comparable to just 2 sec. of visual exploration. Two-handed exploration was significantly superior to one-handed exploration only at the 20-sec. exposure duration. These results illustrate the disparity in the information-gathering capabilities between the visual and haptic systems.", "contents": "Are two hands better than one? assessing information acquired from one- and two-handed haptic exploration of random forms. 15 male undergraduates were presented with 10-sided random forms which they explored visually, or one-handed haptically, or two-handed haptically. Forms were exposed for various durations and subjects were required to draw each form immediately after its removal. The results indicated that 30 sec. of one-handed exploration and 20 and 30 sec. of two-handed exploration produced performance comparable to just 2 sec. of visual exploration. Two-handed exploration was significantly superior to one-handed exploration only at the 20-sec. exposure duration. These results illustrate the disparity in the information-gathering capabilities between the visual and haptic systems.", "PMID": 958802}
{"id": "pubmed23n0032_3255", "title": "Effect of stress on field dependence.", "content": "60 subjects were tested in the rod-and-frame test under flicker conditions (stress). As compared to scores in a control situation (no flicker), the rod-and-frame scores were large under stress and increased monotonically during the session. Futhermore, both intra- and interindividual variability of rod-and-frame performance changed under stress conditions in a consistent manner. The general results, which clearly point to a reliable influence of stress on field dependency, are discussed within the methodological framework of Witkin's theory of perception and personality.", "contents": "Effect of stress on field dependence. 60 subjects were tested in the rod-and-frame test under flicker conditions (stress). As compared to scores in a control situation (no flicker), the rod-and-frame scores were large under stress and increased monotonically during the session. Futhermore, both intra- and interindividual variability of rod-and-frame performance changed under stress conditions in a consistent manner. The general results, which clearly point to a reliable influence of stress on field dependency, are discussed within the methodological framework of Witkin's theory of perception and personality.", "PMID": 958803}
{"id": "pubmed23n0032_3256", "title": "Aftereffects of short-term monocular deprivation on critical flicker frequency of the non-occluded eye.", "content": "Ten subjects were monocularly deprived for a period of 6 hr. The CFF of the non-occluded eye was determined just before deprivation, at 3 and 6 hr. of deprivation and at 1, 3, 5, and 18 hr. following restoration of binocular vision. Results indicated a large depression in CFF at 6 hr. of deprivation followed by a gradual return to baseline that was virtually complete 18 hr. afterr binocular vision was restored. The results were discussed in terms of previous research and theory.", "contents": "Aftereffects of short-term monocular deprivation on critical flicker frequency of the non-occluded eye. Ten subjects were monocularly deprived for a period of 6 hr. The CFF of the non-occluded eye was determined just before deprivation, at 3 and 6 hr. of deprivation and at 1, 3, 5, and 18 hr. following restoration of binocular vision. Results indicated a large depression in CFF at 6 hr. of deprivation followed by a gradual return to baseline that was virtually complete 18 hr. afterr binocular vision was restored. The results were discussed in terms of previous research and theory.", "PMID": 958804}
{"id": "pubmed23n0032_3257", "title": "Boredom and arousal: comparison of tasks differing in visual complexity.", "content": "This experiment was designed to examine the relationship of boredom to arousal. 32 male subjects participated, with 16 subjects performing a low-visual-complexity task and the remainder performing a task high in visual complexity. Both physiological and subjective measures were obtained. Responses to subjective questionnaires showed significant increases in boredom for both groups. Physiological measures indicated a mixed pattern of change. These results suggest a complex response pattern for the construct of boredom which cannot be described as clearly showing either increasing or decreasing arousal.", "contents": "Boredom and arousal: comparison of tasks differing in visual complexity. This experiment was designed to examine the relationship of boredom to arousal. 32 male subjects participated, with 16 subjects performing a low-visual-complexity task and the remainder performing a task high in visual complexity. Both physiological and subjective measures were obtained. Responses to subjective questionnaires showed significant increases in boredom for both groups. Physiological measures indicated a mixed pattern of change. These results suggest a complex response pattern for the construct of boredom which cannot be described as clearly showing either increasing or decreasing arousal.", "PMID": 958805}
{"id": "pubmed23n0032_3258", "title": "Variation of reinforcement in performance of a motor skill.", "content": "A simple experimental procedure administered by parents was used to study  the effects of variation of reinforcement on performance of a motor skill by a 7-yr.-old retarded male. Owing to hemiplegia this subject has a very restricted range of movement in his left hand. The task required him to use his left hand to place wooden cubes within squares drawn on a paper sheet. Factors varied were type of reinforcer (3 consumables), schedule (continuous, fixed ratio and variable ratio), and frequency of reinforcement. Administration of variable ratio delivery was proceived as an enjoyable game by the subject who produced his best performances under this condition. Functional analyses (mini-experiments) should include type of reinforcer and form of delivery.", "contents": "Variation of reinforcement in performance of a motor skill. A simple experimental procedure administered by parents was used to study  the effects of variation of reinforcement on performance of a motor skill by a 7-yr.-old retarded male. Owing to hemiplegia this subject has a very restricted range of movement in his left hand. The task required him to use his left hand to place wooden cubes within squares drawn on a paper sheet. Factors varied were type of reinforcer (3 consumables), schedule (continuous, fixed ratio and variable ratio), and frequency of reinforcement. Administration of variable ratio delivery was proceived as an enjoyable game by the subject who produced his best performances under this condition. Functional analyses (mini-experiments) should include type of reinforcer and form of delivery.", "PMID": 958806}
{"id": "pubmed23n0032_3259", "title": "Information seeking vs information utilization in children's induction from incomplete pictures.", "content": "By removing small squares which completely covered simple silhouette pictures, 4-,6-,8-, and 10-yr.-old children gradually exposed information until they were able to induce, or identify, the whole picture. This method allowed differentiation between the development of information-seeking behavior (accuracy of removing only those squares which exposed the silhouette) and utilization of information (accuracy of identifying partially exposed silhouettes). Results indicated a monotonic development in information seeking but a plateau between ages 6 and 8 yr. in utilization of information.", "contents": "Information seeking vs information utilization in children's induction from incomplete pictures. By removing small squares which completely covered simple silhouette pictures, 4-,6-,8-, and 10-yr.-old children gradually exposed information until they were able to induce, or identify, the whole picture. This method allowed differentiation between the development of information-seeking behavior (accuracy of removing only those squares which exposed the silhouette) and utilization of information (accuracy of identifying partially exposed silhouettes). Results indicated a monotonic development in information seeking but a plateau between ages 6 and 8 yr. in utilization of information.", "PMID": 958807}
{"id": "pubmed23n0032_3260", "title": "Color preference in the gibbon.", "content": "Color preference was tested by showing 6 colored objects to one adult gibbon. The subject demonstrated both color and position preferences on a modified Wisconsin General Test Apparatus. The blue object and, during some conditions, responses to the subject's right were most preferred.", "contents": "Color preference in the gibbon. Color preference was tested by showing 6 colored objects to one adult gibbon. The subject demonstrated both color and position preferences on a modified Wisconsin General Test Apparatus. The blue object and, during some conditions, responses to the subject's right were most preferred.", "PMID": 958808}
{"id": "pubmed23n0032_3261", "title": "Distribution of practice effects on learning retention and relearning by retarded boys.", "content": "The stabilometer task was used to study the difference between massed practice and distributed practice on initial acquisition, retention, and relearning of a gross motor skill by mentally retarded boys. 72 subjects were randomly assigned to either one massed practice group or one of three distributed practice groups with varying interrial rest intervals. All subjects were retested for retention and relearning after 8 wk. of no practice. The results indicated that distributed practice was superior to massed practice for initial skill acquisition. Retention was superior, favoring the group given distributed practice, but no significant differences were found between groups for relearning, indicating that the advantage of distributed practice may be temporary and that it is a performance rather than a learning factor.", "contents": "Distribution of practice effects on learning retention and relearning by retarded boys. The stabilometer task was used to study the difference between massed practice and distributed practice on initial acquisition, retention, and relearning of a gross motor skill by mentally retarded boys. 72 subjects were randomly assigned to either one massed practice group or one of three distributed practice groups with varying interrial rest intervals. All subjects were retested for retention and relearning after 8 wk. of no practice. The results indicated that distributed practice was superior to massed practice for initial skill acquisition. Retention was superior, favoring the group given distributed practice, but no significant differences were found between groups for relearning, indicating that the advantage of distributed practice may be temporary and that it is a performance rather than a learning factor.", "PMID": 958809}
{"id": "pubmed23n0032_3262", "title": "Test of the incidental-cues hypothesis.", "content": "Five groups of adult Ss were submitted to free-recall learning of 24 objects presented as written nouns for the control group and as pictures for experimental groups, as follows: schematic black and white, schematic colored, detailed black and white, and detailed colored drawings. Pictures were better recalled than nouns. Recall was significantly inferior for schematic black and white than for detailed colored drawings, with schematic colored and detailed black and white drawings at an intermediate level. These results support the so-called incidental-cues hypothesis according to which the best recall of pictures in comparison with that of nouns is partially the result of pictures having more distinctive cues likely to be encoded than do verbal items.", "contents": "Test of the incidental-cues hypothesis. Five groups of adult Ss were submitted to free-recall learning of 24 objects presented as written nouns for the control group and as pictures for experimental groups, as follows: schematic black and white, schematic colored, detailed black and white, and detailed colored drawings. Pictures were better recalled than nouns. Recall was significantly inferior for schematic black and white than for detailed colored drawings, with schematic colored and detailed black and white drawings at an intermediate level. These results support the so-called incidental-cues hypothesis according to which the best recall of pictures in comparison with that of nouns is partially the result of pictures having more distinctive cues likely to be encoded than do verbal items.", "PMID": 958810}
{"id": "pubmed23n0032_3263", "title": "Relationship between a behavioral and several psychometric measures of test anxiety.", "content": "Behavioral time-sampling was compared with various paper-and-pencil, self-report measures of test anxiety in an examination of the utility of the behavioral measure as an in situo index of test anxiety. The behavioral measure was significantly and positively correlated 0.45 with a paper-and pencil, self-report measure of \"facilitative\" test anxiety but not with measures of \"debilitative\" test anxiety (r = -0.15) or general anxiety (r = -0.33) for 12 males and 21 female undergraduates.", "contents": "Relationship between a behavioral and several psychometric measures of test anxiety. Behavioral time-sampling was compared with various paper-and-pencil, self-report measures of test anxiety in an examination of the utility of the behavioral measure as an in situo index of test anxiety. The behavioral measure was significantly and positively correlated 0.45 with a paper-and pencil, self-report measure of \"facilitative\" test anxiety but not with measures of \"debilitative\" test anxiety (r = -0.15) or general anxiety (r = -0.33) for 12 males and 21 female undergraduates.", "PMID": 958811}
{"id": "pubmed23n0032_3264", "title": "Frequency of eidetic imagery among hydrocephalic children.", "content": "A sample of 75 mentally retarded children, including 14 with arrested hydrocephalus, 39 with other diagnoses of brain damage, and 22 familial cases, was tested for eidetic imagery, in order to clarify the relationship between eidetric imagery and neuropathology. The hypotheses tested were that the frequency of eidetic imagery is higher among hydrocephalics than among other brain-damaged children and higher among hydrocephalics than among familials. The data confirmed the hypotheses and are interpreted as supporting a theory in which particular structural impairments in the visual system may delay neural development and result in a persistance of eidetic imagery.", "contents": "Frequency of eidetic imagery among hydrocephalic children. A sample of 75 mentally retarded children, including 14 with arrested hydrocephalus, 39 with other diagnoses of brain damage, and 22 familial cases, was tested for eidetic imagery, in order to clarify the relationship between eidetric imagery and neuropathology. The hypotheses tested were that the frequency of eidetic imagery is higher among hydrocephalics than among other brain-damaged children and higher among hydrocephalics than among familials. The data confirmed the hypotheses and are interpreted as supporting a theory in which particular structural impairments in the visual system may delay neural development and result in a persistance of eidetic imagery.", "PMID": 958812}
{"id": "pubmed23n0032_3265", "title": "A signal-detection determination of the two-point supraliminal difference limen on three different body areas.", "content": "This investigation was designed to determine the relationship between the tactual two-point threshold and the two-point supraliminal difference limen. The yes/no method was employed and three localities were tested. The results confirmed the hypothesis that as the initial two-point threshold increases, the two-point supraliminal difference limen also increases. No significant differences were obtained between the right and the left sides of each body area tested and only on the hand was a reliable difference obtained between the first 9 and second 9 sessions. This difference was interpreted in terms of a peripheral fatigue phenomenon.", "contents": "A signal-detection determination of the two-point supraliminal difference limen on three different body areas. This investigation was designed to determine the relationship between the tactual two-point threshold and the two-point supraliminal difference limen. The yes/no method was employed and three localities were tested. The results confirmed the hypothesis that as the initial two-point threshold increases, the two-point supraliminal difference limen also increases. No significant differences were obtained between the right and the left sides of each body area tested and only on the hand was a reliable difference obtained between the first 9 and second 9 sessions. This difference was interpreted in terms of a peripheral fatigue phenomenon.", "PMID": 958813}
{"id": "pubmed23n0032_3266", "title": "Effects of practice and positional variables in acquisition of a complex psychomotor skill.", "content": "40 Navy subjects practiced a three-dimensional tracking task in two positions (the apparatus either in front of,, or above, the subject) under distributed and massed practice schedules to determine the effects of these variables on acquisition of skill. Distributed and massed practice were defined in terms of trial (4 1/2 and 9 min.) and session (1 and 2 hr.) lengths with the subjects equally divided into these four groups: distributed trials, distributed sessions; distributed trials, massed sessions; massed trials, distributed sessions; and massed trials, massed sessions. Total training (practice and rest) time was identical for all groups. Results indicated that task position was a relevant variable in acquisition of skill, i.e. different practice schedules were optimal for the two positions studied.", "contents": "Effects of practice and positional variables in acquisition of a complex psychomotor skill. 40 Navy subjects practiced a three-dimensional tracking task in two positions (the apparatus either in front of,, or above, the subject) under distributed and massed practice schedules to determine the effects of these variables on acquisition of skill. Distributed and massed practice were defined in terms of trial (4 1/2 and 9 min.) and session (1 and 2 hr.) lengths with the subjects equally divided into these four groups: distributed trials, distributed sessions; distributed trials, massed sessions; massed trials, distributed sessions; and massed trials, massed sessions. Total training (practice and rest) time was identical for all groups. Results indicated that task position was a relevant variable in acquisition of skill, i.e. different practice schedules were optimal for the two positions studied.", "PMID": 958815}
{"id": "pubmed23n0032_3267", "title": "Visual observing by rhesus monkeys: influence of potentially threatening stimuli.", "content": "7 rhesus monkeys were afforded the opportunity to observe a series of projected color slides of other monkeys exhibiting five content categories of social-communicative behavior (submission, intense threat, mild threat, grooming, and neutral behavior) combined with two levels of familiarity (familiar vs unfamiliar) with reference to the animals depicted in the slides. Mean duration of observing was significantly influenced by the social-communicative content categories, while mean frequency of observing was significantly affected by the familiarity dimension. The data were discussed in terms of the threat potential or fear-arousing capacity of social stimuli in relation to visual obsrving.", "contents": "Visual observing by rhesus monkeys: influence of potentially threatening stimuli. 7 rhesus monkeys were afforded the opportunity to observe a series of projected color slides of other monkeys exhibiting five content categories of social-communicative behavior (submission, intense threat, mild threat, grooming, and neutral behavior) combined with two levels of familiarity (familiar vs unfamiliar) with reference to the animals depicted in the slides. Mean duration of observing was significantly influenced by the social-communicative content categories, while mean frequency of observing was significantly affected by the familiarity dimension. The data were discussed in terms of the threat potential or fear-arousing capacity of social stimuli in relation to visual obsrving.", "PMID": 958816}
{"id": "pubmed23n0032_3268", "title": "Autonomy of imagery and production of original verbal images.", "content": "90 college students (31 men and 59 women) were categorized as moderately autonomous, less autonomous (less highly controlled) and non-autonomous (high controlled) imagers according to the Gordon Test of Visual Imagery Control Moderately autonomous imagers produced significantly more original verbal images than less autonomous and non-autonomous imagers with less autonomous imagers scoring higher than non-autonomous imagers as measured by Onomatopoeia and Images. There were no significant sex main effects of interaction of autonomy of imagery level X sex.", "contents": "Autonomy of imagery and production of original verbal images. 90 college students (31 men and 59 women) were categorized as moderately autonomous, less autonomous (less highly controlled) and non-autonomous (high controlled) imagers according to the Gordon Test of Visual Imagery Control Moderately autonomous imagers produced significantly more original verbal images than less autonomous and non-autonomous imagers with less autonomous imagers scoring higher than non-autonomous imagers as measured by Onomatopoeia and Images. There were no significant sex main effects of interaction of autonomy of imagery level X sex.", "PMID": 958817}
{"id": "pubmed23n0032_3269", "title": "Personal space, body image and leadership: an exploratory study.", "content": "Personal space and boundary scores of black female leaders and non-leaders were compared (N = 26). Results showed that personal space scores distinguished the groups, while boundary indices did not.", "contents": "Personal space, body image and leadership: an exploratory study. Personal space and boundary scores of black female leaders and non-leaders were compared (N = 26). Results showed that personal space scores distinguished the groups, while boundary indices did not.", "PMID": 958818}
{"id": "pubmed23n0032_3270", "title": "Lateral onset asynchrony in left-handers.", "content": "Study of matching judgment was designed to demonstrate an effect of lateral onset asynchrony in left-handed subjects, 7 males and 8 females. Japanese Hirakana letters or random forms were presented to one visual field first and to the other visual field second. 15 left-handers were requested to judge whether the successively presented stimuli were \"same\" or \"different.\" The results showed that for both types of stimuli there are no differences in accuracy of matching judgement whether the standard stimulus was presented to the right visual field first or to the left. These results indicate that the left-handed subjects may have a tendency toward hemispheric equi-potentiality for recognition of both verbal and non-verbal materials.", "contents": "Lateral onset asynchrony in left-handers. Study of matching judgment was designed to demonstrate an effect of lateral onset asynchrony in left-handed subjects, 7 males and 8 females. Japanese Hirakana letters or random forms were presented to one visual field first and to the other visual field second. 15 left-handers were requested to judge whether the successively presented stimuli were \"same\" or \"different.\" The results showed that for both types of stimuli there are no differences in accuracy of matching judgement whether the standard stimulus was presented to the right visual field first or to the left. These results indicate that the left-handed subjects may have a tendency toward hemispheric equi-potentiality for recognition of both verbal and non-verbal materials.", "PMID": 958820}
{"id": "pubmed23n0032_3271", "title": "Test-retest reliability and interrelation among three locus of control measures for children.", "content": "Test-retest reliability was obtained for the Bialer Scale for Children, the Stranford Preschool I-E Scale and a TAT Locus of Control measure. Correlations between measures ranged from .06 to .26. Based on a lack of correlation between measures, high test-retest reliability for the Bialer, a grade effect on Bialer, and the results of previous studies, the Bialer was viewed as an adequate measure of locus of control for children.", "contents": "Test-retest reliability and interrelation among three locus of control measures for children. Test-retest reliability was obtained for the Bialer Scale for Children, the Stranford Preschool I-E Scale and a TAT Locus of Control measure. Correlations between measures ranged from .06 to .26. Based on a lack of correlation between measures, high test-retest reliability for the Bialer, a grade effect on Bialer, and the results of previous studies, the Bialer was viewed as an adequate measure of locus of control for children.", "PMID": 958821}
{"id": "pubmed23n0032_3272", "title": "Vibrotactile and temperature sensory interaction in the human tongue.", "content": "Lingual vibrotactile thresholds were used to demonstrate an interaction between the lingual senses of vibrotaction and temperture. Thresholds were obtained on 10 Ss from the anterior midline of the lingual dorsum at a frequency of 250 Hz. Five temperature conditions were tested ranging in 10 degrees C steps from 17 degrees C to 57 degrees C. Thresholds were obtained before and after the introduction of the temperature stimuli. Comparison of mean pre- and post-experimental thresholds demonstrated that temperature is a determining factor in lingual vibrotactile threshold values. The data indicated that systematic variation from normal body temperature resulted in corresponding decreases in lingual vibrotactile sensitivity. Diagnostic and clinical implications of this interaction were discussed.", "contents": "Vibrotactile and temperature sensory interaction in the human tongue. Lingual vibrotactile thresholds were used to demonstrate an interaction between the lingual senses of vibrotaction and temperture. Thresholds were obtained on 10 Ss from the anterior midline of the lingual dorsum at a frequency of 250 Hz. Five temperature conditions were tested ranging in 10 degrees C steps from 17 degrees C to 57 degrees C. Thresholds were obtained before and after the introduction of the temperature stimuli. Comparison of mean pre- and post-experimental thresholds demonstrated that temperature is a determining factor in lingual vibrotactile threshold values. The data indicated that systematic variation from normal body temperature resulted in corresponding decreases in lingual vibrotactile sensitivity. Diagnostic and clinical implications of this interaction were discussed.", "PMID": 958822}
{"id": "pubmed23n0032_3273", "title": "Efficacy of the Visual Retention Test as a group-administered instrument for young children.", "content": "Group and individual administrations of the Revised Visual Retention Test to 84 primary-grade children, matched on chronological age and IQ, were demonstrated to be comparable. Form C of the test was used for memory and copy administrations, together with a multiple-choice version. No significant difference was obtained between treatments on memory, copy, or multiple-choice. The group testing procedure appears to provide a valid technique for screening intact classroms for visual perception, memory, and constructive deficits. Further research with the various forms and administrations is suggested to determine the educational correlates of performance.", "contents": "Efficacy of the Visual Retention Test as a group-administered instrument for young children. Group and individual administrations of the Revised Visual Retention Test to 84 primary-grade children, matched on chronological age and IQ, were demonstrated to be comparable. Form C of the test was used for memory and copy administrations, together with a multiple-choice version. No significant difference was obtained between treatments on memory, copy, or multiple-choice. The group testing procedure appears to provide a valid technique for screening intact classroms for visual perception, memory, and constructive deficits. Further research with the various forms and administrations is suggested to determine the educational correlates of performance.", "PMID": 958823}
{"id": "pubmed23n0032_3274", "title": "Age, sex, and delay time as factors affecting reaction to delayed auditory feedback.", "content": "Five groups of 10 males and 10 females each, aged 5, 7, 9, 11, and 13 yr., recited a nursery rhyme under normal delay and 113, 226, 306-m 403-, and 520-msec, delayed auditory-feedback conditions. Speaking rate and disfluency count changes from normal delay to each delayed auditory feedback condition were calculated as indicators of reaction to delayed auditory feedback. Analyses of variance and post hoc comparisons indicated that 5-yr.-olds reacted with greater change in rate at 520-msec. delayed auditory feedback than did older subjects. Five- and 7-yr.-olds were more disfluent at 413-and 520-msec. delayed auditory feedback than were older subjects. Sex differences were found in the 7-, 11-, and 13-yr.-old groups, using speaking rate as a measure of delayed auditory-feedback reaction. No significant sex differences were noted when disfluencies were used as indicators of delayed auditory-feedback reaction.", "contents": "Age, sex, and delay time as factors affecting reaction to delayed auditory feedback. Five groups of 10 males and 10 females each, aged 5, 7, 9, 11, and 13 yr., recited a nursery rhyme under normal delay and 113, 226, 306-m 403-, and 520-msec, delayed auditory-feedback conditions. Speaking rate and disfluency count changes from normal delay to each delayed auditory feedback condition were calculated as indicators of reaction to delayed auditory feedback. Analyses of variance and post hoc comparisons indicated that 5-yr.-olds reacted with greater change in rate at 520-msec. delayed auditory feedback than did older subjects. Five- and 7-yr.-olds were more disfluent at 413-and 520-msec. delayed auditory feedback than were older subjects. Sex differences were found in the 7-, 11-, and 13-yr.-old groups, using speaking rate as a measure of delayed auditory-feedback reaction. No significant sex differences were noted when disfluencies were used as indicators of delayed auditory-feedback reaction.", "PMID": 958824}
{"id": "pubmed23n0032_3275", "title": "Body perception upon awakening.", "content": "12 male subjects were evaluated with a battery of body perception measures before sleep and just after awakening. Personality measures were also obtained. The results indicated a borderline, but not significant, trend at the time of awakening for the body boundary to be reduced in definiteness; and there were sensations that one's body is more dirty, that its openings are more blocked, and that its size is of less expansive proportions. There were also trends for the traits of stability and reflectiveness to predict bodily experiences upon awakening.", "contents": "Body perception upon awakening. 12 male subjects were evaluated with a battery of body perception measures before sleep and just after awakening. Personality measures were also obtained. The results indicated a borderline, but not significant, trend at the time of awakening for the body boundary to be reduced in definiteness; and there were sensations that one's body is more dirty, that its openings are more blocked, and that its size is of less expansive proportions. There were also trends for the traits of stability and reflectiveness to predict bodily experiences upon awakening.", "PMID": 958825}
{"id": "pubmed23n0032_3276", "title": "Shape constancy as a function of selected physical stimulus dimensions.", "content": "By a random method the right half of a figure was constructed; then a mirror image was drawn on the left side to produce a perfectly symmetrical figure. This basic figure was manipulated in such a way as to produce five figures (instances) each on the four physical dimensions of symmetry, number of points, ratio of curved to angled turns, and extension. The hypothesis that the instances of the physical dimensions would be related to degree of shape constancy in an ordinal manner was not supported in any simple way. However there was enough evidence to warrent further investigation. The dimension of extension was selected. Three new stimulus forms were constructed in a manner commensurate with Exp. I, and varied over the dimension of extension. The functions from instance to instance of the three random figures were not significantly similar. However, the homegeneity of mean Brunswik ratios, and individual responses decreased with increased extension; also, values of the ratio closer to 1.00 were generated with increased extension. This suggests that the interaction effects of particular figure and location on the dimension of extension may be of fundamental importance.", "contents": "Shape constancy as a function of selected physical stimulus dimensions. By a random method the right half of a figure was constructed; then a mirror image was drawn on the left side to produce a perfectly symmetrical figure. This basic figure was manipulated in such a way as to produce five figures (instances) each on the four physical dimensions of symmetry, number of points, ratio of curved to angled turns, and extension. The hypothesis that the instances of the physical dimensions would be related to degree of shape constancy in an ordinal manner was not supported in any simple way. However there was enough evidence to warrent further investigation. The dimension of extension was selected. Three new stimulus forms were constructed in a manner commensurate with Exp. I, and varied over the dimension of extension. The functions from instance to instance of the three random figures were not significantly similar. However, the homegeneity of mean Brunswik ratios, and individual responses decreased with increased extension; also, values of the ratio closer to 1.00 were generated with increased extension. This suggests that the interaction effects of particular figure and location on the dimension of extension may be of fundamental importance.", "PMID": 958826}
{"id": "pubmed23n0032_3277", "title": "Heart-rate effects on human reaction time.", "content": "Intra-subject rs between auditory RT and heart rate for 100 males (Mage = 55 yr.) showed RT to be independent of heart rate determined from single bears. rs were of negligible magnitude though more than a chance number were significant.", "contents": "Heart-rate effects on human reaction time. Intra-subject rs between auditory RT and heart rate for 100 males (Mage = 55 yr.) showed RT to be independent of heart rate determined from single bears. rs were of negligible magnitude though more than a chance number were significant.", "PMID": 958827}
{"id": "pubmed23n0032_3278", "title": "An apparatus for indirect measurement of perceived distance.", "content": "Two methods of measuring perceived distance are contrasted. One of these, called a direct method, accepts the observer's direct response to perceived distance as a valid measure of the distance perceived. The other, called an indirect measure, uses the observer's direct response to a perception that is not perceived distance but which has a known relation to perceived distance in order to calculate the distance perceived. There are indications that the direct measure of perceived distance provided by the verbal report sometimes will be modified by cognitive factors. A procedure and apparatus for an indirect measure is suggested which is likely to be free of the cognitive effects found in verbal reports of distance. This apparatus adjusts the distance around which the line-of-sight to the object pivots as the head is moved laterally. The pivot distance at which no apparent motion of the object occurs with head motion is a measure of the perceived distance of the object.", "contents": "An apparatus for indirect measurement of perceived distance. Two methods of measuring perceived distance are contrasted. One of these, called a direct method, accepts the observer's direct response to perceived distance as a valid measure of the distance perceived. The other, called an indirect measure, uses the observer's direct response to a perception that is not perceived distance but which has a known relation to perceived distance in order to calculate the distance perceived. There are indications that the direct measure of perceived distance provided by the verbal report sometimes will be modified by cognitive factors. A procedure and apparatus for an indirect measure is suggested which is likely to be free of the cognitive effects found in verbal reports of distance. This apparatus adjusts the distance around which the line-of-sight to the object pivots as the head is moved laterally. The pivot distance at which no apparent motion of the object occurs with head motion is a measure of the perceived distance of the object.", "PMID": 958828}
{"id": "pubmed23n0032_3279", "title": "Necker cube reversal: sensory or psychological satiation?", "content": "24 students, 5 male and 19 female, were used in a repeated-measures factorial design to test two theories of Necker cube reversal. It was hypothesized that cubes with complete contours and high figure-ground contrast would reverse at faster rates than cubes with incomplete contours and low contrast if a sensory satiation theory (K\u00f6hler & Wallach, 1944) is valid, but at the same rate if the satiation of an orientation theory (Orbach, Ehrlich, & Health, 1963) is correct. High contrast was achieved with black contours on white grounds and vice versa, low contrast with gray contours on black and white grounds. Cubes with complete contours, and stimuli in which only the eight corners of the cube were visible through 18-mm holes superimposed upon the complete cube, provided the contour variable. The results showed a higher reversal rate for cubes with complete contours but no contrast effect. The results were interpreted as supporting a sensory satiation theory.", "contents": "Necker cube reversal: sensory or psychological satiation? 24 students, 5 male and 19 female, were used in a repeated-measures factorial design to test two theories of Necker cube reversal. It was hypothesized that cubes with complete contours and high figure-ground contrast would reverse at faster rates than cubes with incomplete contours and low contrast if a sensory satiation theory (K\u00f6hler & Wallach, 1944) is valid, but at the same rate if the satiation of an orientation theory (Orbach, Ehrlich, & Health, 1963) is correct. High contrast was achieved with black contours on white grounds and vice versa, low contrast with gray contours on black and white grounds. Cubes with complete contours, and stimuli in which only the eight corners of the cube were visible through 18-mm holes superimposed upon the complete cube, provided the contour variable. The results showed a higher reversal rate for cubes with complete contours but no contrast effect. The results were interpreted as supporting a sensory satiation theory.", "PMID": 958829}
{"id": "pubmed23n0032_3280", "title": "Transcendental Meditation and fine perceptual-motor skill.", "content": "30 college male meditators had a 20-min. meditation followed by a 6-min. waking phase prior to 5-min. continuous practice on the pursuit rotor task. This was followed by a 4-min. rest then a further 2-min. of pursuit rotor practice. A similar group of college males who were non-meditators (N = 30) followed the same procedures except that instead of meditating they sat quietly for the initial 20-min. period. The expectations that Transcendental Meditation would (a) facilitate learning and performance; (b) cause less within-subject variability; and (C) cause less reactive inhibition, (c) cause less reactive inhinition, were not upheld by the results. With the exception of performance, which was significantly lower for the meditators, the two groups were no different. Thus, it appears that certain reported physiological and psychological benefits that are attributed to the practice of Transcendental Meditation (such as less anxiety, greater consistency, more awareness, altertness, and attention) are not manifested in the present behavioral test of perceptural-motor function. In fact, in terms of performance, the meditators seemed to be at a disadvantage.", "contents": "Transcendental Meditation and fine perceptual-motor skill. 30 college male meditators had a 20-min. meditation followed by a 6-min. waking phase prior to 5-min. continuous practice on the pursuit rotor task. This was followed by a 4-min. rest then a further 2-min. of pursuit rotor practice. A similar group of college males who were non-meditators (N = 30) followed the same procedures except that instead of meditating they sat quietly for the initial 20-min. period. The expectations that Transcendental Meditation would (a) facilitate learning and performance; (b) cause less within-subject variability; and (C) cause less reactive inhibition, (c) cause less reactive inhinition, were not upheld by the results. With the exception of performance, which was significantly lower for the meditators, the two groups were no different. Thus, it appears that certain reported physiological and psychological benefits that are attributed to the practice of Transcendental Meditation (such as less anxiety, greater consistency, more awareness, altertness, and attention) are not manifested in the present behavioral test of perceptural-motor function. In fact, in terms of performance, the meditators seemed to be at a disadvantage.", "PMID": 958830}
{"id": "pubmed23n0032_3281", "title": "Amount of verbal information and ear-differences in 5- and 6-year old boys and girls.", "content": "Tow experiments were performed. The first was to determine the effects of varying the amount of information in verbal instructions presented monaurally on differences between ears and sex in 5-yr.-old boys and girls. Verbal instructions were given for the performance of a visual-motor task. The results indicated that the amount of verbal information affects ear-asymmetry in girls and not boys. The second experiment was performed to determine the effects of varying the amount of verbal information in verbal instructions presented monaurally on differences between ears, sexes, and reading ability of 6-yr.-old boys and girls. The findings indicated that above average readers performed better than below average readers. Ear-asymmetry was observed across sex and reading abilities for sentences containing 7 and 8 stimulus attributes. No ear-asymmetry was observed in sentences containg 9 stimulus attributes. The results appear to support the progressive lateralization hypothesis which states that ear-asymmetry is dependent on task conditions. The results also support sex differences in the development of brain lateralization.", "contents": "Amount of verbal information and ear-differences in 5- and 6-year old boys and girls. Tow experiments were performed. The first was to determine the effects of varying the amount of information in verbal instructions presented monaurally on differences between ears and sex in 5-yr.-old boys and girls. Verbal instructions were given for the performance of a visual-motor task. The results indicated that the amount of verbal information affects ear-asymmetry in girls and not boys. The second experiment was performed to determine the effects of varying the amount of verbal information in verbal instructions presented monaurally on differences between ears, sexes, and reading ability of 6-yr.-old boys and girls. The findings indicated that above average readers performed better than below average readers. Ear-asymmetry was observed across sex and reading abilities for sentences containing 7 and 8 stimulus attributes. No ear-asymmetry was observed in sentences containg 9 stimulus attributes. The results appear to support the progressive lateralization hypothesis which states that ear-asymmetry is dependent on task conditions. The results also support sex differences in the development of brain lateralization.", "PMID": 958831}
{"id": "pubmed23n0032_3282", "title": "Visual serial search across number and letter sets.", "content": "To discover whether visual serial search time for numbers differed significantly from time for letters, a repeated-measurements design was used with 24 undergraduate women. Performance on number targets 0 to 9 was significantly moe rapid than performance on the letter targets A to J, I to R, or Q to Z. By controlling confounding effects due to redundancy, the possibility that the outcomes might be related to the balance of linear and curvi-linear target features was strengthened.", "contents": "Visual serial search across number and letter sets. To discover whether visual serial search time for numbers differed significantly from time for letters, a repeated-measurements design was used with 24 undergraduate women. Performance on number targets 0 to 9 was significantly moe rapid than performance on the letter targets A to J, I to R, or Q to Z. By controlling confounding effects due to redundancy, the possibility that the outcomes might be related to the balance of linear and curvi-linear target features was strengthened.", "PMID": 958832}
{"id": "pubmed23n0032_3283", "title": "Effects of postinatal stress on visual and auditory evoked potentials.", "content": "Maturation of visual and auditory evoked potentials (mainly the P3 wave) of 10 controls and of 10 infants exposed to recurrent postnatal stressful events (crying spells lacking organic basis) have been compared. The sourse of maturation of P3 waves may serve as indicator of growth and nature of cognitive processes (including perception). Recurrent srying spells seemed to delay appearance of visual P3 wave, prolonged its latency, and delayed appearance of visible differences in the shape of visual P3 wave upon exposure of the subjects to different visual experiences. A compensatory increase of maturation of the auditory P3 wave appeared. The results suggest that recurrent exposure to stressful events during the early postnatal period may delay the ability of the memory banks of auditory engrams with unusual contents (including memory traces of stresses), a potential basis for future auditory hallucinations.", "contents": "Effects of postinatal stress on visual and auditory evoked potentials. Maturation of visual and auditory evoked potentials (mainly the P3 wave) of 10 controls and of 10 infants exposed to recurrent postnatal stressful events (crying spells lacking organic basis) have been compared. The sourse of maturation of P3 waves may serve as indicator of growth and nature of cognitive processes (including perception). Recurrent srying spells seemed to delay appearance of visual P3 wave, prolonged its latency, and delayed appearance of visible differences in the shape of visual P3 wave upon exposure of the subjects to different visual experiences. A compensatory increase of maturation of the auditory P3 wave appeared. The results suggest that recurrent exposure to stressful events during the early postnatal period may delay the ability of the memory banks of auditory engrams with unusual contents (including memory traces of stresses), a potential basis for future auditory hallucinations.", "PMID": 958833}
{"id": "pubmed23n0032_3284", "title": "Music preference as a factor of music distraction.", "content": "The present study is an experimental analysis of music preference as a factor of music distraction measured by the Stroop tests. Significant differences were found amoung groups given, most preferred music, least preferred music, and no music (quiet). Each group of 10 subjects were randomly chosen from 42 screened subjects. Performance was better without music and better with most preferred than with least preferred music. The results were not consistent with those previously found for the effects of noise upon Stroop test performance.", "contents": "Music preference as a factor of music distraction. The present study is an experimental analysis of music preference as a factor of music distraction measured by the Stroop tests. Significant differences were found amoung groups given, most preferred music, least preferred music, and no music (quiet). Each group of 10 subjects were randomly chosen from 42 screened subjects. Performance was better without music and better with most preferred than with least preferred music. The results were not consistent with those previously found for the effects of noise upon Stroop test performance.", "PMID": 958834}
{"id": "pubmed23n0032_3285", "title": "The 'golden section': an artifact of stimulus range and demand characteristics.", "content": "Rectangles haveing different ratios between the lengths of their sides were presented in three different ranges: with the golden section either (a) the next to most elongated rectangle, (b) the middle rectangle, or (c) the next to shortest rectangle. Each of 90 subjects had to pick the rectangles they liked best from all three ranges. This basic procedure was run under three different conditions: (a) subjects did not know the stimuli before, (b) subjects knew them from a foregoing psychophysical experiment, (c) the same, with reinforcing the golden section. The rectangles at both extremes of each range were chosen first most often under the first two conditions, whereas the golden section was chosen most often under the last condition. The conclusion is that preference for the golden section is an artifact of the stimulus range and of the demand characteristics of the experimental procedure rather than of any intrinsic aesthetic quality.", "contents": "The 'golden section': an artifact of stimulus range and demand characteristics. Rectangles haveing different ratios between the lengths of their sides were presented in three different ranges: with the golden section either (a) the next to most elongated rectangle, (b) the middle rectangle, or (c) the next to shortest rectangle. Each of 90 subjects had to pick the rectangles they liked best from all three ranges. This basic procedure was run under three different conditions: (a) subjects did not know the stimuli before, (b) subjects knew them from a foregoing psychophysical experiment, (c) the same, with reinforcing the golden section. The rectangles at both extremes of each range were chosen first most often under the first two conditions, whereas the golden section was chosen most often under the last condition. The conclusion is that preference for the golden section is an artifact of the stimulus range and of the demand characteristics of the experimental procedure rather than of any intrinsic aesthetic quality.", "PMID": 958835}
{"id": "pubmed23n0032_3286", "title": "An audiovisual test of divided attention.", "content": "A description is given of an audiovisual reaction time task, performance of which was maximal after 15 min. practice and which lasted at this level for 1 wk. Sensitivity of the test was shown in an adverse effect of inhalation of traces of anesthetic gases. Comparing responses to auditory and visual stimuli, the control auditory reaction times were faster but were lengthened more in the presence of anesthetics than were the visual ones. This test may be useful in other studies of subtle depressant drug effects.", "contents": "An audiovisual test of divided attention. A description is given of an audiovisual reaction time task, performance of which was maximal after 15 min. practice and which lasted at this level for 1 wk. Sensitivity of the test was shown in an adverse effect of inhalation of traces of anesthetic gases. Comparing responses to auditory and visual stimuli, the control auditory reaction times were faster but were lengthened more in the presence of anesthetics than were the visual ones. This test may be useful in other studies of subtle depressant drug effects.", "PMID": 958836}
{"id": "pubmed23n0032_3287", "title": "Influence of pretest waiting time and duration of induction on kinesthetic aftereffect.", "content": "The influence of pretest hand-resting time and induction time on kinesthetic aftereffect was determined for 22 males and females. Aftereffect was assessed with the apparatus of Koehler and Dinnerstein (1947). Three separate tests of aftereffect were administered. Each was preceded by either a 10-, 20-, or 30-min. hand-resting period. Significant differences in aftereffect were not found between pretest conditions. Scores were also determined for varying induction times at each pretest condition. Induction periods totaling 90-, 180-, and 300-sec. were used. Aftereffect was significantly less pronounced following 90 than 180 sec. of induction for the 10-and 20-min. pretest conditions but was the same following 90, 180, and 300 sec. of induction for the 30-min. condition. Results indicated that less than 300 sec. of induction could be used regardless of the duration of the pretest period. However, because the internal validity of the 180-sec. score was significantly better than the 90-sec. score, it was concluded that at least 180 sec. of induction were needed.", "contents": "Influence of pretest waiting time and duration of induction on kinesthetic aftereffect. The influence of pretest hand-resting time and induction time on kinesthetic aftereffect was determined for 22 males and females. Aftereffect was assessed with the apparatus of Koehler and Dinnerstein (1947). Three separate tests of aftereffect were administered. Each was preceded by either a 10-, 20-, or 30-min. hand-resting period. Significant differences in aftereffect were not found between pretest conditions. Scores were also determined for varying induction times at each pretest condition. Induction periods totaling 90-, 180-, and 300-sec. were used. Aftereffect was significantly less pronounced following 90 than 180 sec. of induction for the 10-and 20-min. pretest conditions but was the same following 90, 180, and 300 sec. of induction for the 30-min. condition. Results indicated that less than 300 sec. of induction could be used regardless of the duration of the pretest period. However, because the internal validity of the 180-sec. score was significantly better than the 90-sec. score, it was concluded that at least 180 sec. of induction were needed.", "PMID": 958837}
{"id": "pubmed23n0032_3288", "title": "Poggendorff illusion as a function of orientation of transversal and parallel lines.", "content": "Adult human subjects (8 male, 8 female undergraduates) with normal vision were required to judge various orientations of the Poggendorff illusion. The transversal and parallel line-segments of the illusion were manipulated to produce the orientations to be judged. Minimum illusion occured when the transversal line-segment was oriented 90 degrees with respect to true vertical or true horizontal. Magnitude of illusion increased as the transversal line-segment deviated from these positions. The findings suggested that there is a stability of horizontal and vertical orientations. In addition, the hypothesis that visual acuity plays a role in the perception of the Poggendorff illusion was proposed.", "contents": "Poggendorff illusion as a function of orientation of transversal and parallel lines. Adult human subjects (8 male, 8 female undergraduates) with normal vision were required to judge various orientations of the Poggendorff illusion. The transversal and parallel line-segments of the illusion were manipulated to produce the orientations to be judged. Minimum illusion occured when the transversal line-segment was oriented 90 degrees with respect to true vertical or true horizontal. Magnitude of illusion increased as the transversal line-segment deviated from these positions. The findings suggested that there is a stability of horizontal and vertical orientations. In addition, the hypothesis that visual acuity plays a role in the perception of the Poggendorff illusion was proposed.", "PMID": 958839}
{"id": "pubmed23n0032_3289", "title": "Stimulus length and orientation variables interact in peripheral motion perception.", "content": "To determine the effects of stimulus length and orientation on the perception of motion, 5 experienced subjects responded with a simple reaction to accelerating lines in peripheral vision while fixating on a reference cross at the center of a cathode-ray tube. Three experimental variables were involved: (a) line length, (b) direction of motion, and (c) orientation of the line with respect to the motion. Simple reaction time (RT) was significantly longer for vertical than for horizontal motion and for lines oriented in-line with the direction of motion than for lines oriented perpendicular to the direction of motion. A significant interaction was found between line length and orientation. The results show that the generalization that RT is shorter for small objects than for large objects must be modified in terms of the orientation of the object.", "contents": "Stimulus length and orientation variables interact in peripheral motion perception. To determine the effects of stimulus length and orientation on the perception of motion, 5 experienced subjects responded with a simple reaction to accelerating lines in peripheral vision while fixating on a reference cross at the center of a cathode-ray tube. Three experimental variables were involved: (a) line length, (b) direction of motion, and (c) orientation of the line with respect to the motion. Simple reaction time (RT) was significantly longer for vertical than for horizontal motion and for lines oriented in-line with the direction of motion than for lines oriented perpendicular to the direction of motion. A significant interaction was found between line length and orientation. The results show that the generalization that RT is shorter for small objects than for large objects must be modified in terms of the orientation of the object.", "PMID": 958840}
{"id": "pubmed23n0032_3290", "title": "Music preference as a factor of music distraction.", "content": "The present study is an experimental analysis of music preference as a factor of music distraction measured by the Stroop tests. Significant differences were found among groups given, most preferred music, least preferred music, and no music(quiet). Each group of 10 subjects were randomly chosen from 42 screened subjects. Performance was better without music and better with most preferred than with least preferred music. The results were not consistent with those previously found for the effects of noise upon Stroop test performance.", "contents": "Music preference as a factor of music distraction. The present study is an experimental analysis of music preference as a factor of music distraction measured by the Stroop tests. Significant differences were found among groups given, most preferred music, least preferred music, and no music(quiet). Each group of 10 subjects were randomly chosen from 42 screened subjects. Performance was better without music and better with most preferred than with least preferred music. The results were not consistent with those previously found for the effects of noise upon Stroop test performance.", "PMID": 958841}
{"id": "pubmed23n0032_3291", "title": "On interocular transfer of the movement aftereffect in indivuals with and without normal binocular vision.", "content": "The duration of the movement aftereffect was measured in twenty-four normally binocular subjects and in eighteen subjects who lacked steropsis as a consequence of childhood strabismus. Aftereffects were generated monocularly and binocularly, and compared to those which occurred after adaption of one eye and testing with the other. Normal subjects were categorized on two indices of eye dominance, which involved sighting and rivalry tests. The monocular-aftereffect durations were slightly longer when the dominant eye was use, and interocular transfer from the dominant eye to the nondominant eye was greater than the transfer in the reverse direction; however, these differences were not statistically significant. The results from the strabismic subjects suggested that they fell into two distinct groups: one group (seven of the eighteen subjects) experienced no interocular transfer in either direction; the group did yield some interocular transfer, and it was generally greater after adaptation of the dominant eye and testing the nondominant eye than in the reverse direction. Six of the seven subjects who failed to show any transfer still had misalignment of the visual axes, but this was not the case in any of the subjects exhibiting transfer.", "contents": "On interocular transfer of the movement aftereffect in indivuals with and without normal binocular vision. The duration of the movement aftereffect was measured in twenty-four normally binocular subjects and in eighteen subjects who lacked steropsis as a consequence of childhood strabismus. Aftereffects were generated monocularly and binocularly, and compared to those which occurred after adaption of one eye and testing with the other. Normal subjects were categorized on two indices of eye dominance, which involved sighting and rivalry tests. The monocular-aftereffect durations were slightly longer when the dominant eye was use, and interocular transfer from the dominant eye to the nondominant eye was greater than the transfer in the reverse direction; however, these differences were not statistically significant. The results from the strabismic subjects suggested that they fell into two distinct groups: one group (seven of the eighteen subjects) experienced no interocular transfer in either direction; the group did yield some interocular transfer, and it was generally greater after adaptation of the dominant eye and testing the nondominant eye than in the reverse direction. Six of the seven subjects who failed to show any transfer still had misalignment of the visual axes, but this was not the case in any of the subjects exhibiting transfer.", "PMID": 958843}
{"id": "pubmed23n0032_3292", "title": "Infant's reaction to changes in orientation of figure and frame.", "content": "Five groups of five-month-old infants observed the same horizontal arrangement of a three-element stimulus placed in a rectangular frame during a series of habituating trials. These trials were followed by either (i) additional exposure to the familiarized standard, (ii) a tringular rearrangement of the three stimulus elements, (iii) oblique rotation of the three elements, (iv) oblique rotation of the three elements and the frame, or (v) oblique rotation of the frame alone. The infants were responsive to all changes in the spatial arrangement of the three elements and to the rotation of the frame, but were less responsive to the change in the frame. The two effects were not additive.", "contents": "Infant's reaction to changes in orientation of figure and frame. Five groups of five-month-old infants observed the same horizontal arrangement of a three-element stimulus placed in a rectangular frame during a series of habituating trials. These trials were followed by either (i) additional exposure to the familiarized standard, (ii) a tringular rearrangement of the three stimulus elements, (iii) oblique rotation of the three elements, (iv) oblique rotation of the three elements and the frame, or (v) oblique rotation of the frame alone. The infants were responsive to all changes in the spatial arrangement of the three elements and to the rotation of the frame, but were less responsive to the change in the frame. The two effects were not additive.", "PMID": 958845}
{"id": "pubmed23n0032_3293", "title": "Steroscopic discrimination in infants.", "content": "The ability to make discrimiations of binocular disparity was investigated in 2-month-old infnats by two methods: (a)fixation preference between patterns differing in the disparity they contained, and (b)recovery from habituation of high-amplitude sucking when there was a change in disparity in the visual reinforcer. The stimuli were random-dot steregrams. The results for both mthods indicated that at least some infants of this age could perform steroscopic discriminations and that both techniques were feasible for development for longitudinal studies of steroscopic vision.", "contents": "Steroscopic discrimination in infants. The ability to make discrimiations of binocular disparity was investigated in 2-month-old infnats by two methods: (a)fixation preference between patterns differing in the disparity they contained, and (b)recovery from habituation of high-amplitude sucking when there was a change in disparity in the visual reinforcer. The stimuli were random-dot steregrams. The results for both mthods indicated that at least some infants of this age could perform steroscopic discriminations and that both techniques were feasible for development for longitudinal studies of steroscopic vision.", "PMID": 958846}
{"id": "pubmed23n0032_3294", "title": "Spatial constraints on focused attention: beyond the right-side advantage.", "content": "The subjects listened to one of two simulataneous synthetic speech syllables delivered independently over two loudspeakers. When the loudspeakers were situated at 90 degrees to the left and to the right, right-side advantage was found. When one loudspeaker was situated in front of the subject in the median plane, and the other at one of several azimuthal positions around him an advantage of the frontal position was observed in all cases. On the other hand, performance on the nonfrontal message was affected significantly by its position. The pattern of performance which is presumably to cerebral dominance, an advantage of sources situated in front of the subject over those at his back, and possibly an advantage of sources near the median plane over more remote ones.", "contents": "Spatial constraints on focused attention: beyond the right-side advantage. The subjects listened to one of two simulataneous synthetic speech syllables delivered independently over two loudspeakers. When the loudspeakers were situated at 90 degrees to the left and to the right, right-side advantage was found. When one loudspeaker was situated in front of the subject in the median plane, and the other at one of several azimuthal positions around him an advantage of the frontal position was observed in all cases. On the other hand, performance on the nonfrontal message was affected significantly by its position. The pattern of performance which is presumably to cerebral dominance, an advantage of sources situated in front of the subject over those at his back, and possibly an advantage of sources near the median plane over more remote ones.", "PMID": 958847}
{"id": "pubmed23n0032_3295", "title": "[Problems raised by the production of spatial models by blind children: an experimental study].", "content": "The ability to reproduct two-dimensional and threee-dimensional spatial models was tested in eighteen blind children, aged seven to eleven with two tasks (block design test and stick test). Performances and strategies were compared with those of seeing children. The results failed to show any important specific errors in blind children; despite blindness some children reached levels of performance as good as those of seeing children, while others completely failed to follow instructions. All such effects were independent of age. The problem is raised of the psychological conditions of space representation, independent of the afferences through which space is perceived.", "contents": "[Problems raised by the production of spatial models by blind children: an experimental study]. The ability to reproduct two-dimensional and threee-dimensional spatial models was tested in eighteen blind children, aged seven to eleven with two tasks (block design test and stick test). Performances and strategies were compared with those of seeing children. The results failed to show any important specific errors in blind children; despite blindness some children reached levels of performance as good as those of seeing children, while others completely failed to follow instructions. All such effects were independent of age. The problem is raised of the psychological conditions of space representation, independent of the afferences through which space is perceived.", "PMID": 958848}
{"id": "pubmed23n0032_3296", "title": "Pigeons learn the concept of an \"A\".", "content": "Pigeons learned to discriminate between \"A\"S and \"2\"s in eighteen different typefaces. They subsequently showed excellent transfer to twenty-two typefaces that they had not previously seen; one pigeon was tested with handwritten letters and responded correctly to them also. Pigeons' responses to \"A's and \"2's with parts removed suggested that their performance was controlled by several features, none of which alone could be considered necessary or sufficient. A test in which birds were shown other letters of the alphabet supported this conclusion. It appears that the original discrimination was learned as what Ryle calls a \"polymorphous concept\".", "contents": "Pigeons learn the concept of an \"A\". Pigeons learned to discriminate between \"A\"S and \"2\"s in eighteen different typefaces. They subsequently showed excellent transfer to twenty-two typefaces that they had not previously seen; one pigeon was tested with handwritten letters and responded correctly to them also. Pigeons' responses to \"A's and \"2's with parts removed suggested that their performance was controlled by several features, none of which alone could be considered necessary or sufficient. A test in which birds were shown other letters of the alphabet supported this conclusion. It appears that the original discrimination was learned as what Ryle calls a \"polymorphous concept\".", "PMID": 958849}
{"id": "pubmed23n0032_3297", "title": "Orientation of the diamond and the square.", "content": "Two experiments show that the young child copies or places diamond and square shapes in alignment with the surrounding frame. The results are related to the difficulty exhibited by young children in drawing diamonds versus squares.", "contents": "Orientation of the diamond and the square. Two experiments show that the young child copies or places diamond and square shapes in alignment with the surrounding frame. The results are related to the difficulty exhibited by young children in drawing diamonds versus squares.", "PMID": 958851}
{"id": "pubmed23n0032_3298", "title": "Intergration and interruption in the masking of form by form.", "content": "Paris of geometric forms of equal area were presented, one form after another, with interstimulus intervals t ranging from 0 to 250 ms. The subject's task in experiment 1 was to recognise both stimuli. Identification of the form presented first (backward masking) across all values of t was of a nonmonotonic nature, with greatest impairment at values of t from 30 to 60 ms. Identification of the second form presented (forward masking) increased monotonically with increasing t. Different forms were recognised with different effectiveness across all values of t where masking took place. The results are interpreted on the basis of a multiple-stage processing model which assumes interaction between stimuli at different levels at different values of t. In experiment 2 the subject's task was to detect the presence or absence of a predesignated form. This condition yielded monotonic functions for both backward and forward masking. An explanation in terms of attention-dependent masking is given.", "contents": "Intergration and interruption in the masking of form by form. Paris of geometric forms of equal area were presented, one form after another, with interstimulus intervals t ranging from 0 to 250 ms. The subject's task in experiment 1 was to recognise both stimuli. Identification of the form presented first (backward masking) across all values of t was of a nonmonotonic nature, with greatest impairment at values of t from 30 to 60 ms. Identification of the second form presented (forward masking) increased monotonically with increasing t. Different forms were recognised with different effectiveness across all values of t where masking took place. The results are interpreted on the basis of a multiple-stage processing model which assumes interaction between stimuli at different levels at different values of t. In experiment 2 the subject's task was to detect the presence or absence of a predesignated form. This condition yielded monotonic functions for both backward and forward masking. An explanation in terms of attention-dependent masking is given.", "PMID": 958852}
{"id": "pubmed23n0032_3299", "title": "Riemannian geometries of variable curvature in visual space: visual alleys, horopters, and triangles in big open fields.", "content": "Luneburg's model for computation of the curvature K of visual two-dimensional space (horizontal visual surface) was tested with equidistant and parallel alleys in large open spaces. Forty-six subjects used stakes to produce 406 experimental alleys of variable sizes (from 5 x 1 to 240 m x 48m). The results show that, contrary to results obtained under laboratory conditions with small alleys and light spots, the individual curvature of visual space does not have negative constant value. K varies in the interval - 1 to + 1 in ninety computed settings: K greater than 0 (N = 38); K less than 0 (N = 52). Therefore the Lobachevskian gemetry currently attributed to visual space ought to be replaced by a Riemannian geometry of variable curvature. Moreover K is an individual function dependent on the size of the alley (distance from the subject), and visual perception would be better understood as scale-dependent. Independently of Luneburg's model we have tested the constancy of the curvature hypothesis in experiments with horopters and visual triangles. The results obtained invalidate Luneburg's hypothesis also.", "contents": "Riemannian geometries of variable curvature in visual space: visual alleys, horopters, and triangles in big open fields. Luneburg's model for computation of the curvature K of visual two-dimensional space (horizontal visual surface) was tested with equidistant and parallel alleys in large open spaces. Forty-six subjects used stakes to produce 406 experimental alleys of variable sizes (from 5 x 1 to 240 m x 48m). The results show that, contrary to results obtained under laboratory conditions with small alleys and light spots, the individual curvature of visual space does not have negative constant value. K varies in the interval - 1 to + 1 in ninety computed settings: K greater than 0 (N = 38); K less than 0 (N = 52). Therefore the Lobachevskian gemetry currently attributed to visual space ought to be replaced by a Riemannian geometry of variable curvature. Moreover K is an individual function dependent on the size of the alley (distance from the subject), and visual perception would be better understood as scale-dependent. Independently of Luneburg's model we have tested the constancy of the curvature hypothesis in experiments with horopters and visual triangles. The results obtained invalidate Luneburg's hypothesis also.", "PMID": 958853}
{"id": "pubmed23n0032_3300", "title": "Psychohysical hallucinations of orientation and spatial frequency.", "content": "After inspection of vertical sinusoidal gratings at least three distinct types of subjective or \"hallucinated\" patterns can be seen on a uniform test field. One type, here called horizontal streaming (H), is already well-known from the work of MacKay. A second type (V) looks like aroughly sinusoidal grating about 1-5 octaves above the adapting spitial frequency. Under optimal conditions a second vertical component appears at about 2 octaves below the adapting frequency. The third category of aftereffect consists of diagonal lines (D) at two orientations (about +/-40 degrees from vertical). The spatial-frequency band at these two orientations appears to be fairly broad, but roughly similar to the adapting frequency. The duration and strength of D increased, while V declined, at higher adapting spatial frequencies. D and V were increasing functions of adapting contrast, while H appeared abruptly only after the highest adapting contrast. H, D, and V are thus all functionally distinct. A schematic model of cortical organization is proposed to account for these phenomena. Pattern channels selective for a given orientation are grouped together with movement channels selective for the orthogonal direction. Antagonism between channels within such \"modules\" accounts for the streaming effect (H). Inhibition between modules tuned to different orientations and spatial frequencies accounts for the D and V effects: after adaptation of channels in one module, neighbouring module(s) are released from inhibition to produce a spurious response which is seen as a grating-like object in the adapted part of the visual field. During flickering adaptation a \"halluncinated\" lattice can be seen superimposed on the adapting grating. It apparently consists of Fourier components more remote from the adapting pattern than D and V are. This disinhibitory effect is strong confirmation of the inhibitory model. The regular and highly organized matrix of channels implied by these experiments may constitute a cortical hypercolumn conducting a coarse, piecewise Fourier transformation of the retinal image.", "contents": "Psychohysical hallucinations of orientation and spatial frequency. After inspection of vertical sinusoidal gratings at least three distinct types of subjective or \"hallucinated\" patterns can be seen on a uniform test field. One type, here called horizontal streaming (H), is already well-known from the work of MacKay. A second type (V) looks like aroughly sinusoidal grating about 1-5 octaves above the adapting spitial frequency. Under optimal conditions a second vertical component appears at about 2 octaves below the adapting frequency. The third category of aftereffect consists of diagonal lines (D) at two orientations (about +/-40 degrees from vertical). The spatial-frequency band at these two orientations appears to be fairly broad, but roughly similar to the adapting frequency. The duration and strength of D increased, while V declined, at higher adapting spatial frequencies. D and V were increasing functions of adapting contrast, while H appeared abruptly only after the highest adapting contrast. H, D, and V are thus all functionally distinct. A schematic model of cortical organization is proposed to account for these phenomena. Pattern channels selective for a given orientation are grouped together with movement channels selective for the orthogonal direction. Antagonism between channels within such \"modules\" accounts for the streaming effect (H). Inhibition between modules tuned to different orientations and spatial frequencies accounts for the D and V effects: after adaptation of channels in one module, neighbouring module(s) are released from inhibition to produce a spurious response which is seen as a grating-like object in the adapted part of the visual field. During flickering adaptation a \"halluncinated\" lattice can be seen superimposed on the adapting grating. It apparently consists of Fourier components more remote from the adapting pattern than D and V are. This disinhibitory effect is strong confirmation of the inhibitory model. The regular and highly organized matrix of channels implied by these experiments may constitute a cortical hypercolumn conducting a coarse, piecewise Fourier transformation of the retinal image.", "PMID": 958854}
{"id": "pubmed23n0032_3301", "title": "Pleuropneumonia caused by Haemophilus parahaemolyticus. An attempt to control the disease at two progeny testing stations by serological blood testing followed by removal of the seropositive animals and their litter mates.", "content": "The complement fixation test was employed at two progeny testing stations in an attempt to avoid outbreaks of contagious pleuropneumonia, caused by Haemophilus parahaemolyticus. Pigs were isolated immediately after arrival at the testing stations and blood samples taken for serology. Test groups with seropositive animals were not admitted to the testing station. The sanitation scheme proved successful, in that acute outbreaks were avoided at both stations and the frequency of chronic pleuritis at slaughter fell from appr. 12 per cent to appr. 6 per cent (Station A) and from appr. 8 per cent to appr. 1 per cent (Station B). Accordingly, the complement fixation test may be of value in diagnosing subclinical infections and thus in the control of Haemophilus parahaemolyticus pleuropneumonia.", "contents": "Pleuropneumonia caused by Haemophilus parahaemolyticus. An attempt to control the disease at two progeny testing stations by serological blood testing followed by removal of the seropositive animals and their litter mates. The complement fixation test was employed at two progeny testing stations in an attempt to avoid outbreaks of contagious pleuropneumonia, caused by Haemophilus parahaemolyticus. Pigs were isolated immediately after arrival at the testing stations and blood samples taken for serology. Test groups with seropositive animals were not admitted to the testing station. The sanitation scheme proved successful, in that acute outbreaks were avoided at both stations and the frequency of chronic pleuritis at slaughter fell from appr. 12 per cent to appr. 6 per cent (Station A) and from appr. 8 per cent to appr. 1 per cent (Station B). Accordingly, the complement fixation test may be of value in diagnosing subclinical infections and thus in the control of Haemophilus parahaemolyticus pleuropneumonia.", "PMID": 958866}
{"id": "pubmed23n0032_3302", "title": "[Incidence of MMA after prostaglandin-induced parturition (author's transl)].", "content": "In a sowherd suffering from high incidence of MMA which demanded an intensive postparturient treatment to reduce baby pig mortality the effect on postparturient diseases of induction of parturition with Prostaglandin was examined. After treatment of 19 sows on day 112 of gestation with 12.5 mg Prostaglandin, parturition in 15 sows took place on an average of 25 hours after injection; the gestation period was 113.1 +/- 0.5 days. Three sows that did not respond to the treatment farrowed 3-6 days after treatment and one sow farrowed few hours after treatment. In a non-treated identical group of animals the gestation period averaged 113.4 +/- 1.5 days. No difference was found between the two groups concerning number of stillborn pgis, baby pig mortality within first eight days of life, incidence of MMA or intensity of postparturient medical treatment.", "contents": "[Incidence of MMA after prostaglandin-induced parturition (author's transl)]. In a sowherd suffering from high incidence of MMA which demanded an intensive postparturient treatment to reduce baby pig mortality the effect on postparturient diseases of induction of parturition with Prostaglandin was examined. After treatment of 19 sows on day 112 of gestation with 12.5 mg Prostaglandin, parturition in 15 sows took place on an average of 25 hours after injection; the gestation period was 113.1 +/- 0.5 days. Three sows that did not respond to the treatment farrowed 3-6 days after treatment and one sow farrowed few hours after treatment. In a non-treated identical group of animals the gestation period averaged 113.4 +/- 1.5 days. No difference was found between the two groups concerning number of stillborn pgis, baby pig mortality within first eight days of life, incidence of MMA or intensity of postparturient medical treatment.", "PMID": 958868}
{"id": "pubmed23n0032_3303", "title": "[A simple flotation and McMaster method (author's transl)].", "content": "A simple and hygienic flotation and McMaster method is described (cf. Fig. 1): The faeces sample (3-5 gm) is mixed with flotation fluid (40-60 ml) in a disposable plastic cup (cup I) and a circular piece of gauze, with a diameter equivalent to twice the height of the cup plus the diameter of its base, is placed over the cup. By a similar cup (cup II), with the bottom cut off and slits made in the side, the gauze is pressed down in cup I. By this procedure the faeces suspension will be filtered through the gauze and appear in cup II. After 10 minutes some drops after transferred from the surface of the filtrate to a slide by means of a test tube, which is dipped to the bottom of the filtrate and quickly moved over to the slide. The optimal procedure was worked out with faeces containing faeces containing eggs of Ostertagia ostertagi. Saturated sodium chloride with 50 g glucose per 100 ml was found to be a suitable flotation fluid for demonstrating eggs of commonly occurring parasites, e.g., trichostronglyids and ascarids. The described flotation technique is more efficacious than the one hitherto employed. The technique is usable also in connection with a McMaster method: 4 gm. faeces are suspended in 56 ml flotation fluid. Immediately following filtration, filtrate is transferred to a McMaster counting chamber by means of a Pasteur pipette.", "contents": "[A simple flotation and McMaster method (author's transl)]. A simple and hygienic flotation and McMaster method is described (cf. Fig. 1): The faeces sample (3-5 gm) is mixed with flotation fluid (40-60 ml) in a disposable plastic cup (cup I) and a circular piece of gauze, with a diameter equivalent to twice the height of the cup plus the diameter of its base, is placed over the cup. By a similar cup (cup II), with the bottom cut off and slits made in the side, the gauze is pressed down in cup I. By this procedure the faeces suspension will be filtered through the gauze and appear in cup II. After 10 minutes some drops after transferred from the surface of the filtrate to a slide by means of a test tube, which is dipped to the bottom of the filtrate and quickly moved over to the slide. The optimal procedure was worked out with faeces containing faeces containing eggs of Ostertagia ostertagi. Saturated sodium chloride with 50 g glucose per 100 ml was found to be a suitable flotation fluid for demonstrating eggs of commonly occurring parasites, e.g., trichostronglyids and ascarids. The described flotation technique is more efficacious than the one hitherto employed. The technique is usable also in connection with a McMaster method: 4 gm. faeces are suspended in 56 ml flotation fluid. Immediately following filtration, filtrate is transferred to a McMaster counting chamber by means of a Pasteur pipette.", "PMID": 958869}
{"id": "pubmed23n0032_3304", "title": "Pleuropneumonia of swine caused by Haemophilus parahaemolyticus. Studies on the protection obtained by vaccination.", "content": "Sixty-six pigs were vaccinated with formaldehyde-killed 24-hour or 6-hour culture of H. parahaemolyticus with Freund's incomplete adjuvant (1:1 or 3:1) or 15 per cent Alhydrogel. The vaccine was injected subcutaneously when the pigs were 9 weeks old. A booster injection was given 2 weeks later. Two weeks after the booster injection the pigs were challenged by intranasal inoculation of 10(10) organisms. The efficacy of the vaccine was evaluated by clinical observations and autopsy findings. Vaccines with Alhydrogel adjuvant: With 24-hour culture 25 per cent of the pigs were protected. With 6-hour culture the protection reached 67 per cent. Vaccines with Freund's incomplete adjuvant: With 24-hour culture 67 per cent of the vaccinates were protected. With 6-hour culture 90 per cent were protected. It is concluded that a 6-hour culture of H. parahaemolyticus is superior to an older culture in immunizing power. Vaccine with Freund's incomplete adjuvant caused severe local reaction with persistent granuloma formation. For that reason it is questionable whether this vaccine will be accepted for field use. H. parahaemolyticus could be found in the respiratory tract, also of animals that were protected by vaccination. Further studies on the significance and duration of the carrier state in this infection are required.", "contents": "Pleuropneumonia of swine caused by Haemophilus parahaemolyticus. Studies on the protection obtained by vaccination. Sixty-six pigs were vaccinated with formaldehyde-killed 24-hour or 6-hour culture of H. parahaemolyticus with Freund's incomplete adjuvant (1:1 or 3:1) or 15 per cent Alhydrogel. The vaccine was injected subcutaneously when the pigs were 9 weeks old. A booster injection was given 2 weeks later. Two weeks after the booster injection the pigs were challenged by intranasal inoculation of 10(10) organisms. The efficacy of the vaccine was evaluated by clinical observations and autopsy findings. Vaccines with Alhydrogel adjuvant: With 24-hour culture 25 per cent of the pigs were protected. With 6-hour culture the protection reached 67 per cent. Vaccines with Freund's incomplete adjuvant: With 24-hour culture 67 per cent of the vaccinates were protected. With 6-hour culture 90 per cent were protected. It is concluded that a 6-hour culture of H. parahaemolyticus is superior to an older culture in immunizing power. Vaccine with Freund's incomplete adjuvant caused severe local reaction with persistent granuloma formation. For that reason it is questionable whether this vaccine will be accepted for field use. H. parahaemolyticus could be found in the respiratory tract, also of animals that were protected by vaccination. Further studies on the significance and duration of the carrier state in this infection are required.", "PMID": 958865}
{"id": "pubmed23n0032_3305", "title": "Experience of the efficacy of equine influenza vaccinations.", "content": "In 1974, a very extensive influenza/A/equi 2 epidemic broke out in the Finnish horse population. To study the efficacy of influenza vaccinations a questionnaire was sent after the epidemic to all Finnish veterinarians. The answer material was selected to contain only stables which had had clinically typical cases. The material consisted 234 unvaccinated and 629 vaccinated horses. In the latter group 466 horses were vaccinated adequately. The results of the study showed that of the unvaccinated horses 212 (91%) and of the adequately vaccinated horses only 42 (9.4%) contracted clinically typical influenza. On the other hand, in the group of horses vaccinated irregularly or only once before the epidemic the disease prevalence was about the same as for the unvaccinated animals. Thus, the equine influenza vaccines used in Finland had a surprising good efficacy if they were used according to instructions.", "contents": "Experience of the efficacy of equine influenza vaccinations. In 1974, a very extensive influenza/A/equi 2 epidemic broke out in the Finnish horse population. To study the efficacy of influenza vaccinations a questionnaire was sent after the epidemic to all Finnish veterinarians. The answer material was selected to contain only stables which had had clinically typical cases. The material consisted 234 unvaccinated and 629 vaccinated horses. In the latter group 466 horses were vaccinated adequately. The results of the study showed that of the unvaccinated horses 212 (91%) and of the adequately vaccinated horses only 42 (9.4%) contracted clinically typical influenza. On the other hand, in the group of horses vaccinated irregularly or only once before the epidemic the disease prevalence was about the same as for the unvaccinated animals. Thus, the equine influenza vaccines used in Finland had a surprising good efficacy if they were used according to instructions.", "PMID": 958867}
{"id": "pubmed23n0032_3306", "title": "[Value of the fractionation of serum immunoglobulins for the diagnosis of rubella infection in pregnant women. Critical study].", "content": "The authors present the results of fractionation by ultracentrifugation in the identification of rubella antibodies in 20 vaccinated children and 389 pregnant women suspected of having rubella. Testing for specific IgM was constantly positive when an exanthematous disease was seen in association with a significant increase an antibody level as measured by standard techniques, making questionable the reed of fractionation under such circumstances. By contrast, however, the shortest delay in collection of the first sample of serum renders fractionation essential. Alone, it made possible the diagnostic of rubella in patients in whom the first serum, although collected within 3 to 5 days after the rash, had the same antibody level as a second serum collected later. Whilst fractionisation requested for contact only without rash did not reveal specific IgM, positive results were obtained rarely in sera with a high antibody level on routine examination. The very uncertain significance of these last results, the frequency of cases of recent rubella with very modest antibody levels and the occasional finding of duodtful results, render the authors highly sceptical as to the value of fractionisation in the presence of a high serum level on routine examination in a pregnant woman.", "contents": "[Value of the fractionation of serum immunoglobulins for the diagnosis of rubella infection in pregnant women. Critical study]. The authors present the results of fractionation by ultracentrifugation in the identification of rubella antibodies in 20 vaccinated children and 389 pregnant women suspected of having rubella. Testing for specific IgM was constantly positive when an exanthematous disease was seen in association with a significant increase an antibody level as measured by standard techniques, making questionable the reed of fractionation under such circumstances. By contrast, however, the shortest delay in collection of the first sample of serum renders fractionation essential. Alone, it made possible the diagnostic of rubella in patients in whom the first serum, although collected within 3 to 5 days after the rash, had the same antibody level as a second serum collected later. Whilst fractionisation requested for contact only without rash did not reveal specific IgM, positive results were obtained rarely in sera with a high antibody level on routine examination. The very uncertain significance of these last results, the frequency of cases of recent rubella with very modest antibody levels and the occasional finding of duodtful results, render the authors highly sceptical as to the value of fractionisation in the presence of a high serum level on routine examination in a pregnant woman.", "PMID": 958871}
{"id": "pubmed23n0032_3307", "title": "[A phonomecanographic test of hyperthyroidism].", "content": "Systolic intervals may be studied by phonomechanography. This provides data regarding the function of the left ventricle. In the absence of any problem of intraventricular conduction or valvular lesion, such information is particularly valuable. Thyroxine impregnation, whetHer active directly and/or by catecholamine hypersecretion, results in cardiac hyperkinesia which is reflected not only by tachycardia but also a shortening penetration time and contraction of the ventricular muscle. This shows itself within systole as a change in the relation between the duration of the pre-ejection phase (PPE) and the ejection phase. The first decreases whilst the second remains normal and their ratio changes, PPE/E changes from a normal value of 0.34 +/- 0.04 to 0.25 +/- 0.03. These tests are not only of value in diagnosis but also make it possible to observe the effects of treatment. It is certain that a true phonomechanographic syndrome of cardiac hyperkinesia may be described. The commonest, though not the only, aetiology is hyperthyroidism.", "contents": "[A phonomecanographic test of hyperthyroidism]. Systolic intervals may be studied by phonomechanography. This provides data regarding the function of the left ventricle. In the absence of any problem of intraventricular conduction or valvular lesion, such information is particularly valuable. Thyroxine impregnation, whetHer active directly and/or by catecholamine hypersecretion, results in cardiac hyperkinesia which is reflected not only by tachycardia but also a shortening penetration time and contraction of the ventricular muscle. This shows itself within systole as a change in the relation between the duration of the pre-ejection phase (PPE) and the ejection phase. The first decreases whilst the second remains normal and their ratio changes, PPE/E changes from a normal value of 0.34 +/- 0.04 to 0.25 +/- 0.03. These tests are not only of value in diagnosis but also make it possible to observe the effects of treatment. It is certain that a true phonomechanographic syndrome of cardiac hyperkinesia may be described. The commonest, though not the only, aetiology is hyperthyroidism.", "PMID": 958878}
{"id": "pubmed23n0032_3308", "title": "[Death or absence of the father. Its role in the genesis of psychotic process happening between 15 and 25 years].", "content": "A retrospective study of the case records of 215 patients developing an acute or chronic psychotic process between the ages of 15 and 25 years indicated that the father had died or disappeared during childhood in 25% of cases, a figure of 7% being found for a control group - a highly significant statistical difference. By contrast, there was no statistically significant difference in the disappearance of the mother between the two groups. The date of death (or disappearance) of the father occurred at three particular periods - at birth, towards the age of 5 and at 11 or 12 years. The authors' discussion concerns the convergence of the results of this statistical approach of the past history of psychotic patients with certain psychodynamic interpretations of psychosis.", "contents": "[Death or absence of the father. Its role in the genesis of psychotic process happening between 15 and 25 years]. A retrospective study of the case records of 215 patients developing an acute or chronic psychotic process between the ages of 15 and 25 years indicated that the father had died or disappeared during childhood in 25% of cases, a figure of 7% being found for a control group - a highly significant statistical difference. By contrast, there was no statistically significant difference in the disappearance of the mother between the two groups. The date of death (or disappearance) of the father occurred at three particular periods - at birth, towards the age of 5 and at 11 or 12 years. The authors' discussion concerns the convergence of the results of this statistical approach of the past history of psychotic patients with certain psychodynamic interpretations of psychosis.", "PMID": 958879}
{"id": "pubmed23n0032_3309", "title": "Ring current shielding effects in nucleic acid double helices.", "content": "Values of ring current shielding in parts-per-million have been calculated for double helical nucleic acids in the A-RNA (RNA-11), A' -RNA (RNA-12) and B-DNA geometries. Atomic coordinates determined previously from x-ray diffraction of fibers were used to calculate the positions of protons relative to both nearest and second nearest neighboring bases, including those on the complementary strand. The magnitude of the diamagnetic shielding was then calculated for each aromatic ring. From these calculations tables were constructed for use in determining the shielding expected for carbon-bound and ring nitrogen-bound protons of any double helical nucleic acid sequence. The results are compared with available experimental data for several oligonucleotides and with previous ring current shielding calculations where differences of up to 0.4 ppm are found.", "contents": "Ring current shielding effects in nucleic acid double helices. Values of ring current shielding in parts-per-million have been calculated for double helical nucleic acids in the A-RNA (RNA-11), A' -RNA (RNA-12) and B-DNA geometries. Atomic coordinates determined previously from x-ray diffraction of fibers were used to calculate the positions of protons relative to both nearest and second nearest neighboring bases, including those on the complementary strand. The magnitude of the diamagnetic shielding was then calculated for each aromatic ring. From these calculations tables were constructed for use in determining the shielding expected for carbon-bound and ring nitrogen-bound protons of any double helical nucleic acid sequence. The results are compared with available experimental data for several oligonucleotides and with previous ring current shielding calculations where differences of up to 0.4 ppm are found.", "PMID": 958893}
{"id": "pubmed23n0032_3310", "title": "DNA-dependent RNA polymerases from Artemia salina. Subunit structure of polymerase II.", "content": "RNA polymerase II from larvae of the brine shrimp, Artemia salina, was highly purified by two cycles of DEAE-cellulose chromatography followed by centrifugation through discontinuous sucrose gradients. Gradient fractions were subjected to elctrophoresis is polyacrylamide gels containing sodium dodecyl sulfate. The subunit structure of RNA polymerase II was determined by quantitative comparison of the polypeptides and enzyme activity present in each gradient fraction. The enzyme contains one copy of each of four subunits with estimated molecular weights of 170,000, 130,000, 36,000 and 24,000. The total molecular weight agrees well with the molecular weight estimated for the native enzyme by density gradient centrifugation.", "contents": "DNA-dependent RNA polymerases from Artemia salina. Subunit structure of polymerase II. RNA polymerase II from larvae of the brine shrimp, Artemia salina, was highly purified by two cycles of DEAE-cellulose chromatography followed by centrifugation through discontinuous sucrose gradients. Gradient fractions were subjected to elctrophoresis is polyacrylamide gels containing sodium dodecyl sulfate. The subunit structure of RNA polymerase II was determined by quantitative comparison of the polypeptides and enzyme activity present in each gradient fraction. The enzyme contains one copy of each of four subunits with estimated molecular weights of 170,000, 130,000, 36,000 and 24,000. The total molecular weight agrees well with the molecular weight estimated for the native enzyme by density gradient centrifugation.", "PMID": 958894}
{"id": "pubmed23n0032_3311", "title": "Nucleosomes in metaphase chromosomes.", "content": "Previous studies of the structure of metaphase chromosomes have relied heavily on electron micrography and have revealed the existence of a 10-nm unit fiber that is thought to generate the native 23-30-nm fiber by higher order folding. The structural relationship of these metaphase fibers to the interphase fiber remains obscure. Recent studies on the digestion of interphase chromatin have revealed the existence of a regularly repeating subunit of DNA and histone, the nucleosome that generates the appearance of 10-nm beads connected by a short fiber of DNA seen on electron micrographs. It was therefore of interest to probe the structure of the metaphase  chromosome for the presence of nucleosomal subunits. To this end metaphase  chromosomes were prepared from colchicine-arrested cultures of mouse L-cells and were subjected to digestion with stayphylococcal nuclease. Comparison of the early and limit digestion products of metaphase chromosomes with those obtained from interphase nuclei indicates that although significant morphologic changes occur within the chromatin fiber during mitosis, the basic subunit structure of the chromatin fiber is retained by the mitotic chromosome.", "contents": "Nucleosomes in metaphase chromosomes. Previous studies of the structure of metaphase chromosomes have relied heavily on electron micrography and have revealed the existence of a 10-nm unit fiber that is thought to generate the native 23-30-nm fiber by higher order folding. The structural relationship of these metaphase fibers to the interphase fiber remains obscure. Recent studies on the digestion of interphase chromatin have revealed the existence of a regularly repeating subunit of DNA and histone, the nucleosome that generates the appearance of 10-nm beads connected by a short fiber of DNA seen on electron micrographs. It was therefore of interest to probe the structure of the metaphase  chromosome for the presence of nucleosomal subunits. To this end metaphase  chromosomes were prepared from colchicine-arrested cultures of mouse L-cells and were subjected to digestion with stayphylococcal nuclease. Comparison of the early and limit digestion products of metaphase chromosomes with those obtained from interphase nuclei indicates that although significant morphologic changes occur within the chromatin fiber during mitosis, the basic subunit structure of the chromatin fiber is retained by the mitotic chromosome.", "PMID": 958895}
{"id": "pubmed23n0032_3312", "title": "Duplex structure formation between oligo(dA)'s and oligo(dT)'s generated by thymine-specific interaction with netropsin.", "content": "The formation of oligomeric duplex molecules in the presence of the antibiotic netropsin in the series p(dA)n-p(dT)n is demonstrated using low-temperature CD measurements. Addition of Netropsin to mixtures of oligomers generates the same type of CD spectra as observed for poly(dA)-poly(dT) and maintains the duplex structure at temperatures at which base pairing of free oligomers is thermodynamically unstable. The shortest chain length forming a netropsin complex by thymine-specific interaction with the oligopeptide is represented by p(dA)4-p(dt)4. Studies with sequence isomers show that adjacent thymine residues strongly favour the complex formation with the oligopeptide.", "contents": "Duplex structure formation between oligo(dA)'s and oligo(dT)'s generated by thymine-specific interaction with netropsin. The formation of oligomeric duplex molecules in the presence of the antibiotic netropsin in the series p(dA)n-p(dT)n is demonstrated using low-temperature CD measurements. Addition of Netropsin to mixtures of oligomers generates the same type of CD spectra as observed for poly(dA)-poly(dT) and maintains the duplex structure at temperatures at which base pairing of free oligomers is thermodynamically unstable. The shortest chain length forming a netropsin complex by thymine-specific interaction with the oligopeptide is represented by p(dA)4-p(dt)4. Studies with sequence isomers show that adjacent thymine residues strongly favour the complex formation with the oligopeptide.", "PMID": 958896}
{"id": "pubmed23n0032_3313", "title": "[Demonstration total brain infarct with radioisotope angiography].", "content": "In the field of organ transplantation and in brain death patients where intensive-care measures may seem superfluous, the demonstration of cessation of cerebral blood flow by X-ray angiography is generally agreed to be the diagnostic procedure of choice to prove irreversible loss of cerebral function. There are, however, certain drawbacks involved in X-ray angiography. Arterial puncture is necessary. Furthermore, the procedure can be time-consuming, thus making the continuation of adequate intensive-care measures more difficult. At the same time the circulatory condition may worsen causing hypoxic damage to the organ to be transplanted. In the present paper, the authors report on 13 patients with clinical signs of brain death where cessation of cerebral blood flow was demonstrated atraumatically by intravenous radioisotope angiography (RIA) using a multicrystal gammacamera (Baird Atomic) and the bolus-injection technique with 99m Tc-pertechnetate. Nine patients had severe brain injuries, 2 patients had brain tumours, 1 patient had encephalitis and 1 patient had suffered prepartal thrombosis of the sinus sagittalis. In all patients EEG recordings were isoelectric. At the time when the RIA was performed systolic blood pressure had decreased to 62-85 mmHg (x = 71 mmHg), while body temperature had declined to 31-36,5 degrees C (x = 34 degrees). According to the present results, which were all confirmed by subsequent bilateral carotid X-ray angiography, total brain infarction is unequivocal when the following criteria are satisfied using RIA: 1. when the radioisotope bolus flows along the common carotid arteries but does not proceed any further than to the base of the skull or around the scalp structures, 2. when, at the moment when the radioactivity outlines the scalp structures, neither the intracranial arteries nor the capillary bed or the venous sinuses are visible, 3. when the time-activity curves across the hemispheres show simply a plateau of low count rate without the activity peak typical for cerebral tracer circulation and 4. when the activity peak, typical for venous outflow, is missing from the time-activity curves for the cervical areas. In 12 patients with extremely reduced cerebral blood flow it was demonstrated that the RIA findings were clearly different from those obtained at brain death. Moreover, not one of 438 other patients undergoing RIA exhibited the same features which were associated with brain death. The authors conclude that RIA involves the same degree of safety as X-ray angiography in the diagnosis of total brain infarction but is superior to the latter when the diagnostic procedure has to be performed quickly, thus reducing the risk of any further damage to a prospective donor organ.", "contents": "[Demonstration total brain infarct with radioisotope angiography]. In the field of organ transplantation and in brain death patients where intensive-care measures may seem superfluous, the demonstration of cessation of cerebral blood flow by X-ray angiography is generally agreed to be the diagnostic procedure of choice to prove irreversible loss of cerebral function. There are, however, certain drawbacks involved in X-ray angiography. Arterial puncture is necessary. Furthermore, the procedure can be time-consuming, thus making the continuation of adequate intensive-care measures more difficult. At the same time the circulatory condition may worsen causing hypoxic damage to the organ to be transplanted. In the present paper, the authors report on 13 patients with clinical signs of brain death where cessation of cerebral blood flow was demonstrated atraumatically by intravenous radioisotope angiography (RIA) using a multicrystal gammacamera (Baird Atomic) and the bolus-injection technique with 99m Tc-pertechnetate. Nine patients had severe brain injuries, 2 patients had brain tumours, 1 patient had encephalitis and 1 patient had suffered prepartal thrombosis of the sinus sagittalis. In all patients EEG recordings were isoelectric. At the time when the RIA was performed systolic blood pressure had decreased to 62-85 mmHg (x = 71 mmHg), while body temperature had declined to 31-36,5 degrees C (x = 34 degrees). According to the present results, which were all confirmed by subsequent bilateral carotid X-ray angiography, total brain infarction is unequivocal when the following criteria are satisfied using RIA: 1. when the radioisotope bolus flows along the common carotid arteries but does not proceed any further than to the base of the skull or around the scalp structures, 2. when, at the moment when the radioactivity outlines the scalp structures, neither the intracranial arteries nor the capillary bed or the venous sinuses are visible, 3. when the time-activity curves across the hemispheres show simply a plateau of low count rate without the activity peak typical for cerebral tracer circulation and 4. when the activity peak, typical for venous outflow, is missing from the time-activity curves for the cervical areas. In 12 patients with extremely reduced cerebral blood flow it was demonstrated that the RIA findings were clearly different from those obtained at brain death. Moreover, not one of 438 other patients undergoing RIA exhibited the same features which were associated with brain death. The authors conclude that RIA involves the same degree of safety as X-ray angiography in the diagnosis of total brain infarction but is superior to the latter when the diagnostic procedure has to be performed quickly, thus reducing the risk of any further damage to a prospective donor organ.", "PMID": 958897}
{"id": "pubmed23n0032_3314", "title": "In vitro determination of unbound and total iron-binding capacity in serum by radioiron using a new ion exchange strip.", "content": "A simplified method for the in vitro determination of TIBC and UIBC in serum using 59Fe was subjected to quality control in the laboratory and clinically studied in duplicate determinations of 185 sera of an unselected patient population. The data obtained were investigated by statistical means (regression, correlation, variance analysis). The precision of the method was evaluated step by step. The results indicate that the radiochemical determination of TIBC values is equivalent to the \"indirect\" one (UIBC + SI) as far as accuracy is concerned. Advantages of the radiochemical method are discussed.", "contents": "In vitro determination of unbound and total iron-binding capacity in serum by radioiron using a new ion exchange strip. A simplified method for the in vitro determination of TIBC and UIBC in serum using 59Fe was subjected to quality control in the laboratory and clinically studied in duplicate determinations of 185 sera of an unselected patient population. The data obtained were investigated by statistical means (regression, correlation, variance analysis). The precision of the method was evaluated step by step. The results indicate that the radiochemical determination of TIBC values is equivalent to the \"indirect\" one (UIBC + SI) as far as accuracy is concerned. Advantages of the radiochemical method are discussed.", "PMID": 958898}
{"id": "pubmed23n0032_3315", "title": "Significance of renal visualization during bone scanning in Paget's disease.", "content": "In 17 of 26 patients with extensive, symptomatic Paget's disease poor renal visualization was noted on bone scanning with 99mTc-diphosphonate. Renal function was normal in all patients. The intensity of the renal image proved to be inversely related to the extent and metabolic activity of the Pagetic process. This finding supports the hypothesis that in Paget's disease the balance between skeletal and renal extraction of circulating tracer amy be displaced in favor of the former.", "contents": "Significance of renal visualization during bone scanning in Paget's disease. In 17 of 26 patients with extensive, symptomatic Paget's disease poor renal visualization was noted on bone scanning with 99mTc-diphosphonate. Renal function was normal in all patients. The intensity of the renal image proved to be inversely related to the extent and metabolic activity of the Pagetic process. This finding supports the hypothesis that in Paget's disease the balance between skeletal and renal extraction of circulating tracer amy be displaced in favor of the former.", "PMID": 958899}
{"id": "pubmed23n0032_3316", "title": "[Thyroid hormones and thyrotropin in liver and kidney insufficiency].", "content": "In 22 patients with hepatic or renal insufficiency the serum concentrations of trijodothyronin, thyroxine and thyrotropin and also the T4-binding capacity of TBG were determined. The mean serum T3 concentration was found to be significantly lower in patients with hepatic coma when compared with euthyroid subjects. In the cases of renal insufficiency the serum T3 concentrations were in the normal range. Due to hormone loss through dialysis however, the mean value of the T3 concentrations was slightly lower than the average concentration of normal subjects. The obtained results agree with those of our earlier studies which showed that there are significant differences between liver artery and vein T3 concentrations in serum, whereas no such differences could be ascertained between serum concentrations in renal artery and vein. On the basis of these findings it is assumed that conversion of T4 into T3 occurs predominantly in the liver.", "contents": "[Thyroid hormones and thyrotropin in liver and kidney insufficiency]. In 22 patients with hepatic or renal insufficiency the serum concentrations of trijodothyronin, thyroxine and thyrotropin and also the T4-binding capacity of TBG were determined. The mean serum T3 concentration was found to be significantly lower in patients with hepatic coma when compared with euthyroid subjects. In the cases of renal insufficiency the serum T3 concentrations were in the normal range. Due to hormone loss through dialysis however, the mean value of the T3 concentrations was slightly lower than the average concentration of normal subjects. The obtained results agree with those of our earlier studies which showed that there are significant differences between liver artery and vein T3 concentrations in serum, whereas no such differences could be ascertained between serum concentrations in renal artery and vein. On the basis of these findings it is assumed that conversion of T4 into T3 occurs predominantly in the liver.", "PMID": 958900}
{"id": "pubmed23n0032_3317", "title": "[Albumin kinetics in superior mesenteric artery shock in rats].", "content": "1. After 1-hour-tourniquet of the arteria mesenterical superior of the rat the result is a loss of intravasal fluid into the small intestine. After 2-hour-tourniquet the loss of fluid into the samll intestine can be higher than the volume of plasma circulating at the start of the experiment. During therapy with plasma substitute on base of gelatine (Haemaccel) the volume of content of the small intestine increases further. 2. After 1-hour-tourniquet the content of intravasal albumin is diminished by 26%, after 2-hour-tourniquet by 46%. Under therapy with a plasma substitute the loss of intravasal albumin increases further. 3. After 1-hour-tourniquet a transient accumulation of albumin was found in the wall of the samll intestine which was reduced after 2-hour-tourniquet. 4. The intravasal albumin is excreted in the small and the large intestine. After 1-hour-tourniquet 46% of excreted albumin was found in the small intestine after 2-hour-tourniquet 61%. It can be demonstrated, that under replacement with plasma substitute the result is mainly an \"albumin dilution effect\". The wall of the small intestine becomes permeable to high-molecular weight substances with an average molecular weight below 60.000. An effective therapy with the plasma substitute of the AMS-shock should, therefore, contain carrier substances with a molecular weight higher than 60.000.", "contents": "[Albumin kinetics in superior mesenteric artery shock in rats]. 1. After 1-hour-tourniquet of the arteria mesenterical superior of the rat the result is a loss of intravasal fluid into the small intestine. After 2-hour-tourniquet the loss of fluid into the samll intestine can be higher than the volume of plasma circulating at the start of the experiment. During therapy with plasma substitute on base of gelatine (Haemaccel) the volume of content of the small intestine increases further. 2. After 1-hour-tourniquet the content of intravasal albumin is diminished by 26%, after 2-hour-tourniquet by 46%. Under therapy with a plasma substitute the loss of intravasal albumin increases further. 3. After 1-hour-tourniquet a transient accumulation of albumin was found in the wall of the samll intestine which was reduced after 2-hour-tourniquet. 4. The intravasal albumin is excreted in the small and the large intestine. After 1-hour-tourniquet 46% of excreted albumin was found in the small intestine after 2-hour-tourniquet 61%. It can be demonstrated, that under replacement with plasma substitute the result is mainly an \"albumin dilution effect\". The wall of the small intestine becomes permeable to high-molecular weight substances with an average molecular weight below 60.000. An effective therapy with the plasma substitute of the AMS-shock should, therefore, contain carrier substances with a molecular weight higher than 60.000.", "PMID": 958902}
{"id": "pubmed23n0032_3318", "title": "Absorbed radiation dose from radionuclidic impurities in several 123I-labeled radiopharmaceuticals.", "content": "The radiation dose to patients from RISA, sodium iodohippurate, and rose bengal labeled with commercially available 123I and its accompanying radioimpurities has been studied. The extra radiation dose to target organs was found to range from 29% to 97% that due to 123I alone when the radiopharmaceutical is administered at calibration time. Additional delay in the time of administration increases the relative impurity dose.", "contents": "Absorbed radiation dose from radionuclidic impurities in several 123I-labeled radiopharmaceuticals. The radiation dose to patients from RISA, sodium iodohippurate, and rose bengal labeled with commercially available 123I and its accompanying radioimpurities has been studied. The extra radiation dose to target organs was found to range from 29% to 97% that due to 123I alone when the radiopharmaceutical is administered at calibration time. Additional delay in the time of administration increases the relative impurity dose.", "PMID": 958903}
{"id": "pubmed23n0032_3319", "title": "Lung morphology in experimental uremia.", "content": "The investigations were performed on 15 dogs with experimental uremia, but free of overhydration and circulatory insufficiency. Parahilar lungs areas showed numerous atelectatic foci, the peripheral part--conpensatory marginal emphusema. Light microscopy revealed a decreased number of granular cells, electron microscopy the \"empty\" appearance of the lamellar structures, and decrease or absence of the Hale-positive layer of alveolar lining film. The inhibition of cells controlling the alveolar surgace tension seems to be crucial in the pathomechanism of development of uremic lung lesions.", "contents": "Lung morphology in experimental uremia. The investigations were performed on 15 dogs with experimental uremia, but free of overhydration and circulatory insufficiency. Parahilar lungs areas showed numerous atelectatic foci, the peripheral part--conpensatory marginal emphusema. Light microscopy revealed a decreased number of granular cells, electron microscopy the \"empty\" appearance of the lamellar structures, and decrease or absence of the Hale-positive layer of alveolar lining film. The inhibition of cells controlling the alveolar surgace tension seems to be crucial in the pathomechanism of development of uremic lung lesions.", "PMID": 959040}
{"id": "pubmed23n0032_3320", "title": "Serum insulin, pancreatic glucagon and growth hormone levels in response to intravenous infusion of L-arginine in patients with recently detected juvenile diabetes.", "content": "The secretion of insulin, glucagon and growth hormone was determined in the serum of patients with recently diagnosed juvenile-type diabetes (10 patients) during stimulation by intravenous infusion of L-arginine and was compared with the results found in a group of five healthy persons. The value of the insulinemia was significantly lower in the diabetics as compared with the healthy controls. Serum glucagon levels were higher in all diabetics when fasting and after L-arginine administration as compared with the controls but a significant difference was observed only at the peck of secretion (5 min after L-arginine administration). Growth hormone concentration was slightly higher in the diabetics after secretory stimulation than in the controls, particularly at the peak of secretion (30 and 45 min) but the difference was statistically no significant.", "contents": "Serum insulin, pancreatic glucagon and growth hormone levels in response to intravenous infusion of L-arginine in patients with recently detected juvenile diabetes. The secretion of insulin, glucagon and growth hormone was determined in the serum of patients with recently diagnosed juvenile-type diabetes (10 patients) during stimulation by intravenous infusion of L-arginine and was compared with the results found in a group of five healthy persons. The value of the insulinemia was significantly lower in the diabetics as compared with the healthy controls. Serum glucagon levels were higher in all diabetics when fasting and after L-arginine administration as compared with the controls but a significant difference was observed only at the peck of secretion (5 min after L-arginine administration). Growth hormone concentration was slightly higher in the diabetics after secretory stimulation than in the controls, particularly at the peak of secretion (30 and 45 min) but the difference was statistically no significant.", "PMID": 959042}
{"id": "pubmed23n0032_3321", "title": "Scanning electron microscopy in medico-legal investigations of hair.", "content": "The appearance of the hair surface as revealed by scanning electron microscopy is described. Intact human and animal hair, hair after shot and hair treated with thermic energy were the subject of investigation. The authors paid attention to numerous details of the pictures obtained due to high resolving power of scanning electron microscope.", "contents": "Scanning electron microscopy in medico-legal investigations of hair. The appearance of the hair surface as revealed by scanning electron microscopy is described. Intact human and animal hair, hair after shot and hair treated with thermic energy were the subject of investigation. The authors paid attention to numerous details of the pictures obtained due to high resolving power of scanning electron microscope.", "PMID": 959039}
{"id": "pubmed23n0032_3322", "title": "Secretion of growth hormone in the insulin test in various forms of diabetes.", "content": "In 25 diabetics and 8 controls the insulin hypoglycemia test was performed with subsequent determination of growth hormone secretion by the radioimmunoassay method. The rise of the growth hormone level began earlier and persisted longer in diabetics as compared with controls. Juvenile diabetes was associated with a rapid secretory response of the hormone while in maturity-type diabetes the release of growth hormone in response to stimulation was excessive but delayed. A somewhat lower secretory response was found in diabetes lasting over 5 years as compared with short-lasting diabetes. The observed phenomena were not related to the absolute blood glucose level. Although the phenomenon of growth hormone hypersecretion remains yet to be explained, it seems, however, to be secondary to carbohydrate metabolism disturbance and insulin disorders.", "contents": "Secretion of growth hormone in the insulin test in various forms of diabetes. In 25 diabetics and 8 controls the insulin hypoglycemia test was performed with subsequent determination of growth hormone secretion by the radioimmunoassay method. The rise of the growth hormone level began earlier and persisted longer in diabetics as compared with controls. Juvenile diabetes was associated with a rapid secretory response of the hormone while in maturity-type diabetes the release of growth hormone in response to stimulation was excessive but delayed. A somewhat lower secretory response was found in diabetes lasting over 5 years as compared with short-lasting diabetes. The observed phenomena were not related to the absolute blood glucose level. Although the phenomenon of growth hormone hypersecretion remains yet to be explained, it seems, however, to be secondary to carbohydrate metabolism disturbance and insulin disorders.", "PMID": 959043}
{"id": "pubmed23n0032_3323", "title": "Secretion of growth hormone in the insulin test, and the development of diabetic microangiopathy.", "content": "Serum growth hormone (HGH) was determined in: 1) diabetics without retinopathy, 2) diabetics with retinopathy and normal creatinine clearance. In diabetes without retinopathy the HGH was higher than in controls, while in the group of cases with retinopathy the HGH level was significantly lower than in the group without vascular changes and in the control group. Cases with retinal vascular changes and reduced creatinine clearance had a significantly higher HGH level as compared with the group with normal clearance, and the dynamics of HGH secretion in the group with reduced clearance differed from those in the remaining groups. This pattern of HGH secretion in the studied groups showed a connection between raised HGH level and impairment of renal function developing when vascular changes appear in diabetics.", "contents": "Secretion of growth hormone in the insulin test, and the development of diabetic microangiopathy. Serum growth hormone (HGH) was determined in: 1) diabetics without retinopathy, 2) diabetics with retinopathy and normal creatinine clearance. In diabetes without retinopathy the HGH was higher than in controls, while in the group of cases with retinopathy the HGH level was significantly lower than in the group without vascular changes and in the control group. Cases with retinal vascular changes and reduced creatinine clearance had a significantly higher HGH level as compared with the group with normal clearance, and the dynamics of HGH secretion in the group with reduced clearance differed from those in the remaining groups. This pattern of HGH secretion in the studied groups showed a connection between raised HGH level and impairment of renal function developing when vascular changes appear in diabetics.", "PMID": 959044}
{"id": "pubmed23n0032_3324", "title": "Hyperlipoproteinemia (acc. to Fredrickson's classification) in patients with myocardial infarction and imminent infarction.", "content": "In 500 cases (379 men and 121 women) of ischemic heart disease, including 428 patients with myocardial infarction and 72 -- with imminent infarction, blood lipid levels were determined. Blood samples were taken on fasting, by the end of hospitalization, from patients with myocardial infarction on the 18th-21st day of disease, and from those with imminent infarction after cessation of pain. In 152 patients (30.4%), including 87 with infarction and 65 with imminent infarction, disturbances in the blood lipid pattern were found and classified according to Fredrickson. When expressed as percentage against the material of 152 patients with blood lipid disturbances, type II A was found in 103 cases (67.8%), type II B -- in 25 cases (16.4%), and type IV -- in 24 cases (15.8%). When calculated against the whole material of 500 patients, type II A accounted for 20.6%, type II B -- for 5%, and type IV -- 4.8% of cases.", "contents": "Hyperlipoproteinemia (acc. to Fredrickson's classification) in patients with myocardial infarction and imminent infarction. In 500 cases (379 men and 121 women) of ischemic heart disease, including 428 patients with myocardial infarction and 72 -- with imminent infarction, blood lipid levels were determined. Blood samples were taken on fasting, by the end of hospitalization, from patients with myocardial infarction on the 18th-21st day of disease, and from those with imminent infarction after cessation of pain. In 152 patients (30.4%), including 87 with infarction and 65 with imminent infarction, disturbances in the blood lipid pattern were found and classified according to Fredrickson. When expressed as percentage against the material of 152 patients with blood lipid disturbances, type II A was found in 103 cases (67.8%), type II B -- in 25 cases (16.4%), and type IV -- in 24 cases (15.8%). When calculated against the whole material of 500 patients, type II A accounted for 20.6%, type II B -- for 5%, and type IV -- 4.8% of cases.", "PMID": 959045}
{"id": "pubmed23n0032_3325", "title": "Prognostic value of central venous pressure in acute myocardial infarction.", "content": "The investigations were carried out in 296 patients with myocardial infarction (238 men and 58 women) aged 24-91 years, admitted to an intensive care unit on the 1st or 2nd day of the disease. In all patients the catheter for determination of central venous pressure was introduced into the right atrium through the cephalic vein the the first 100 cases, and through the subclavian vein in 196 cases. The catheter was kept in the atrium for 3-4 days. Values of c.v.p. between 50 and 120 mm Hg were accepted as normal.", "contents": "Prognostic value of central venous pressure in acute myocardial infarction. The investigations were carried out in 296 patients with myocardial infarction (238 men and 58 women) aged 24-91 years, admitted to an intensive care unit on the 1st or 2nd day of the disease. In all patients the catheter for determination of central venous pressure was introduced into the right atrium through the cephalic vein the the first 100 cases, and through the subclavian vein in 196 cases. The catheter was kept in the atrium for 3-4 days. Values of c.v.p. between 50 and 120 mm Hg were accepted as normal.", "PMID": 959046}
{"id": "pubmed23n0032_3326", "title": "Effect of inderal on insulin secretion, glycemia and FFA concentration in normal subjects and diabetics.", "content": "In 20 cases of adult-type diabetes and in 10 healthy controls insulinemia, glycemia and free acids concentration were determined under basal conditions, after glucose load and after administration of inderal and glucose. After intravenous injection of inderal basal insulinemia fell in controls but this fall was statistically not significant, while in diabetics no evident changes of insulinemia were observed. Following inderal injection and oral glucose a statistically significant drop in insulinemia was observed in controls but not in diabetics. In both groups inderal injection was without any significant effect on glycemia before as well as after glucose load. In both groups a significant decrease occurred in the level of free fatty acids and inderal had no effect on it.", "contents": "Effect of inderal on insulin secretion, glycemia and FFA concentration in normal subjects and diabetics. In 20 cases of adult-type diabetes and in 10 healthy controls insulinemia, glycemia and free acids concentration were determined under basal conditions, after glucose load and after administration of inderal and glucose. After intravenous injection of inderal basal insulinemia fell in controls but this fall was statistically not significant, while in diabetics no evident changes of insulinemia were observed. Following inderal injection and oral glucose a statistically significant drop in insulinemia was observed in controls but not in diabetics. In both groups inderal injection was without any significant effect on glycemia before as well as after glucose load. In both groups a significant decrease occurred in the level of free fatty acids and inderal had no effect on it.", "PMID": 959041}
{"id": "pubmed23n0032_3327", "title": "Prognostication of the results of immunosuppressive treatment by means of logical dendrite.", "content": "A logical dendrite was designed for establishing indications for immunosupressive treatment; it was applied in a group of 51 patients with rheumatoid arthritis. A nomogram was constructed for calculation of the expected therapeutic result, on the grounds of the indications, proposed duration of treatment and drug dosage. Comparison of the calculated and actual clinical results of treatment showed that in over 80% of cases the actual results were either consistent with or better than the expected ones. The application of prognostication seems to be particularly useful in the case of long-term treatment with highly toxic agents.", "contents": "Prognostication of the results of immunosuppressive treatment by means of logical dendrite. A logical dendrite was designed for establishing indications for immunosupressive treatment; it was applied in a group of 51 patients with rheumatoid arthritis. A nomogram was constructed for calculation of the expected therapeutic result, on the grounds of the indications, proposed duration of treatment and drug dosage. Comparison of the calculated and actual clinical results of treatment showed that in over 80% of cases the actual results were either consistent with or better than the expected ones. The application of prognostication seems to be particularly useful in the case of long-term treatment with highly toxic agents.", "PMID": 959047}
{"id": "pubmed23n0032_3328", "title": "Reaction of lymphocytes to phytohemagglutinin in vitro in hereditary microspherocytosis.", "content": "The authors report results of investigations on PHA-stimulated blastic transformation of lymphocytes in hereditary microspherocytosis. In 25 patients with this disease including 12 splenectomized cases and 13 non-splenectomized ones a significant decrease of the blastic and mitotic indexes was observed in relation to a control group of 20 subjects. No statistically significant differences were noted, however, between the cases before and after splenectomy. The results indicate that splenectomy is not the only factor impairing blastic transformation in these patients. The authors suggest that in microspherocytosis changes exist not only in the erythrocyte membrane, but that similar changes in the lymphocyte cell membrane may impair the response to phytohemagglutinin (PHA).", "contents": "Reaction of lymphocytes to phytohemagglutinin in vitro in hereditary microspherocytosis. The authors report results of investigations on PHA-stimulated blastic transformation of lymphocytes in hereditary microspherocytosis. In 25 patients with this disease including 12 splenectomized cases and 13 non-splenectomized ones a significant decrease of the blastic and mitotic indexes was observed in relation to a control group of 20 subjects. No statistically significant differences were noted, however, between the cases before and after splenectomy. The results indicate that splenectomy is not the only factor impairing blastic transformation in these patients. The authors suggest that in microspherocytosis changes exist not only in the erythrocyte membrane, but that similar changes in the lymphocyte cell membrane may impair the response to phytohemagglutinin (PHA).", "PMID": 959048}
{"id": "pubmed23n0032_3329", "title": "Cytochemical studies of peripheral blood granulocytes and lymphocytes in patients with systemic lupus erythematosus.", "content": "Using cytoenzymatic methods the activity of acid and alkaline phosphatase, non-specific esterase and glycogen content in peripheral blood cells were analyzed. A statistically significant rise in the activity of these enzymes was demonstrated in granulocytes and lymphocytes and a low level of glycogen in lymphocytes. Following prednisone treatment a statistically significant decrease was observed in the activity of studied enzymes and a rise in glycogen content.", "contents": "Cytochemical studies of peripheral blood granulocytes and lymphocytes in patients with systemic lupus erythematosus. Using cytoenzymatic methods the activity of acid and alkaline phosphatase, non-specific esterase and glycogen content in peripheral blood cells were analyzed. A statistically significant rise in the activity of these enzymes was demonstrated in granulocytes and lymphocytes and a low level of glycogen in lymphocytes. Following prednisone treatment a statistically significant decrease was observed in the activity of studied enzymes and a rise in glycogen content.", "PMID": 959049}
{"id": "pubmed23n0032_3330", "title": "Effect of antiphlogistic steroids on urinary hydroxyproline excretion in systemic lupus erythematosus.", "content": "Total hydroxyproline excretion in 24-hour urine was determined in 20 cases of systemic lupus erythematosus (SLE) before and during treatment. Additionally the excretion of hydroxyproline was determined in 14 patients receiving steroids for various other diseases (endocarditis, thrombocytopenia, acute leukemia, apofocal polyarthritis, ischialgia, spastic bronchitis, pleuritis and Dressler's post-infarction syndrome). The normal hydroxyproline value was established in 40 healthy persons. The normalization of pathologically increased hydroxyproline excretion in patients with SLE during treatment with prednisone in doses from 1.0 to 1.35 mg/kg/daily was associated with remission of clinical symptoms. Large doses of prednisone in pathological processes not primarily affecting connective tissue increased the 24-hour excretion of hydroxyproline and small doses of prednisone had no effect on hydroxyprolinuria.", "contents": "Effect of antiphlogistic steroids on urinary hydroxyproline excretion in systemic lupus erythematosus. Total hydroxyproline excretion in 24-hour urine was determined in 20 cases of systemic lupus erythematosus (SLE) before and during treatment. Additionally the excretion of hydroxyproline was determined in 14 patients receiving steroids for various other diseases (endocarditis, thrombocytopenia, acute leukemia, apofocal polyarthritis, ischialgia, spastic bronchitis, pleuritis and Dressler's post-infarction syndrome). The normal hydroxyproline value was established in 40 healthy persons. The normalization of pathologically increased hydroxyproline excretion in patients with SLE during treatment with prednisone in doses from 1.0 to 1.35 mg/kg/daily was associated with remission of clinical symptoms. Large doses of prednisone in pathological processes not primarily affecting connective tissue increased the 24-hour excretion of hydroxyproline and small doses of prednisone had no effect on hydroxyprolinuria.", "PMID": 959050}
{"id": "pubmed23n0032_3331", "title": "Experimental studies on the growth of cultured epithelium and endothelium of rabbit cornea exposed to low temperature.", "content": "On the basis of the investigations performed it was shown that cryoapplication of the cornea by the original Krwawicz's method influences temporarily the proliferative and migratory ability to epithelial and endothelial corneal cells in tissue culture. The endothelium is more sensitive to the action of low temperature and recovers its normal dynamics of growth 10--12 days after application of cold while the epithelium requires for this only 4--5 days. The ability of migration, DNA synthesis and mitosis in conditions of tissue culture of the corneal epithelium and endothelium, after their exposure to low temperature, are not abolished, but they are suppressed for a longer period than the duration of the described histopathological lesions.", "contents": "Experimental studies on the growth of cultured epithelium and endothelium of rabbit cornea exposed to low temperature. On the basis of the investigations performed it was shown that cryoapplication of the cornea by the original Krwawicz's method influences temporarily the proliferative and migratory ability to epithelial and endothelial corneal cells in tissue culture. The endothelium is more sensitive to the action of low temperature and recovers its normal dynamics of growth 10--12 days after application of cold while the epithelium requires for this only 4--5 days. The ability of migration, DNA synthesis and mitosis in conditions of tissue culture of the corneal epithelium and endothelium, after their exposure to low temperature, are not abolished, but they are suppressed for a longer period than the duration of the described histopathological lesions.", "PMID": 959051}
{"id": "pubmed23n0032_3332", "title": "Allergic alveolar diseases. Problems in diagnosis and management.", "content": "One of the diagnostic possibilities to consider when a patient presents with cough, fever, dyspnea, or pulmonary infiltrates is hypersensitivity pneumonitis. Some of the problems encountered in diagnosis of diffuse lung disease are illustrated in two case reports. In one of the cases, interstitial pneumonitis of insidious onset was attributed to inhalation of thermophilic organisms in moldy silage. In the other, the outstanding pathologic feature was bronchiolitis obliterans, and circumstantial evidence pointed to a home humidifier as the source of the problem.", "contents": "Allergic alveolar diseases. Problems in diagnosis and management. One of the diagnostic possibilities to consider when a patient presents with cough, fever, dyspnea, or pulmonary infiltrates is hypersensitivity pneumonitis. Some of the problems encountered in diagnosis of diffuse lung disease are illustrated in two case reports. In one of the cases, interstitial pneumonitis of insidious onset was attributed to inhalation of thermophilic organisms in moldy silage. In the other, the outstanding pathologic feature was bronchiolitis obliterans, and circumstantial evidence pointed to a home humidifier as the source of the problem.", "PMID": 959074}
{"id": "pubmed23n0032_3333", "title": "Lung biopsy through the flexible fiberoptic bronchoscope.", "content": "Peripheral lung lesions are a difficult diagnostic problem. The technique of forceps lung biopsy through the flexible fiberoptic bronchoscope provides potential access to the entire lung. Experience to date documents the procedure's safety and high yield of helpful information.", "contents": "Lung biopsy through the flexible fiberoptic bronchoscope. Peripheral lung lesions are a difficult diagnostic problem. The technique of forceps lung biopsy through the flexible fiberoptic bronchoscope provides potential access to the entire lung. Experience to date documents the procedure's safety and high yield of helpful information.", "PMID": 959076}
{"id": "pubmed23n0032_3334", "title": "Office gynecology: fibroids-when not to operate.", "content": "The mere presence of uterine fibroids is not, since qua non, an indication for their surgical removal. Knowing when not to operate is probably more important for the physician than knowing when to operate. It is easy to fall into diagnostic and therapeutic traps when uterine fibroids are known to be present, especially when they occur concomitantly with a condition such as irregular uterine bleeding, infertility, or pregnancy. The contributions of such conditions to problems in diagnosing and managing fibroids must be kept in mind by the prudent physician; a decision in favor of medical rather than surgical management is often best.", "contents": "Office gynecology: fibroids-when not to operate. The mere presence of uterine fibroids is not, since qua non, an indication for their surgical removal. Knowing when not to operate is probably more important for the physician than knowing when to operate. It is easy to fall into diagnostic and therapeutic traps when uterine fibroids are known to be present, especially when they occur concomitantly with a condition such as irregular uterine bleeding, infertility, or pregnancy. The contributions of such conditions to problems in diagnosing and managing fibroids must be kept in mind by the prudent physician; a decision in favor of medical rather than surgical management is often best.", "PMID": 959080}
{"id": "pubmed23n0032_3335", "title": "Pediatrics: cervical adenopathy in children.", "content": "Cervical adenopathies are common in children. The four categories, based on clinical findings are acute unilateral adenitis, acute bilateral adenitis, subacute (chronic) adenitis, and cervical node cancers. Most cases of acute pyogenic adenitis-both unilateral and bilateral-respond well to early antibiotic treatment. Penicillin is the drug of choice unless Staphylococcus aureus is thought to be the infecting organism. S aureus is trated with dicloxacillin or erythromycin. Subacute adenitis due to cat scratch fever usually resolves spontaneously. Nodes due to infection with atypical mycobacteria require excision. Because cervical node cancer is usually asymptomatic, excision and pathologic study should be done for any cervical node persistently enlarged without apparent reason.", "contents": "Pediatrics: cervical adenopathy in children. Cervical adenopathies are common in children. The four categories, based on clinical findings are acute unilateral adenitis, acute bilateral adenitis, subacute (chronic) adenitis, and cervical node cancers. Most cases of acute pyogenic adenitis-both unilateral and bilateral-respond well to early antibiotic treatment. Penicillin is the drug of choice unless Staphylococcus aureus is thought to be the infecting organism. S aureus is trated with dicloxacillin or erythromycin. Subacute adenitis due to cat scratch fever usually resolves spontaneously. Nodes due to infection with atypical mycobacteria require excision. Because cervical node cancer is usually asymptomatic, excision and pathologic study should be done for any cervical node persistently enlarged without apparent reason.", "PMID": 959081}
{"id": "pubmed23n0032_3336", "title": "Therapy of the hyperlipidemias.", "content": "Although the diagnosis of hyperlipidemia is somewhat arbitrary, in that the upper limits of normal are not universally agreed upon, it is clear that the risk of atherosclerosis increases with plasma cholesterol concentration; it may also increase in hyperglyceridemia. We take 220 mg/100 ml as the upper limit of normal for plasma cholesterol and 140 mg/100 ml as the upper limit for triglycerides. Treatment of hyperlipidemia is aimed at reducing the risk of atherosclerosis or arresting its course. Alteration of diet is the first step. If maximal diet therapy does not lower plasma lipids to acceptable levels, drug therapy is added. The decision to treat hyperlipidemia with drugs should be based on a careful weighing of risk vs. benefit for each patient. Because of the rapid change in our knowledge of both the risks and the benefits of therapy, that decision must be reviewed and updated at regular intervals.", "contents": "Therapy of the hyperlipidemias. Although the diagnosis of hyperlipidemia is somewhat arbitrary, in that the upper limits of normal are not universally agreed upon, it is clear that the risk of atherosclerosis increases with plasma cholesterol concentration; it may also increase in hyperglyceridemia. We take 220 mg/100 ml as the upper limit of normal for plasma cholesterol and 140 mg/100 ml as the upper limit for triglycerides. Treatment of hyperlipidemia is aimed at reducing the risk of atherosclerosis or arresting its course. Alteration of diet is the first step. If maximal diet therapy does not lower plasma lipids to acceptable levels, drug therapy is added. The decision to treat hyperlipidemia with drugs should be based on a careful weighing of risk vs. benefit for each patient. Because of the rapid change in our knowledge of both the risks and the benefits of therapy, that decision must be reviewed and updated at regular intervals.", "PMID": 959087}
{"id": "pubmed23n0032_3337", "title": "[Effects of dextran, gelatine and homologous plasma on circulation in shock caused by severe anemia in the dog].", "content": "Irreversible shock is produced in 28 anaesthetized mongrel dogs by withdrawal of erythrocytes and substitution by dextran, gelatine or plasma to an average Hb of 4.5g%. In contrast to other shock models cardiac output and perfusion pressure are not decreased by this preparation. In order to maintain arterial blood pressue above 90 mm Hg significantly more gelatine than dextran or serum has to be infused. There is no difference in hemodynamic responses after treatment with dextran or gelatine respectively. Both groups treated with colloidal solutions have the same survival rate of 38%. In the serum substituted dogs, however,survival rate is significantly greater (83%). Very likely this is due to a greater buffer capacity preventing severe acidosis, which is observed in the animals treated with colloidal solutions.", "contents": "[Effects of dextran, gelatine and homologous plasma on circulation in shock caused by severe anemia in the dog]. Irreversible shock is produced in 28 anaesthetized mongrel dogs by withdrawal of erythrocytes and substitution by dextran, gelatine or plasma to an average Hb of 4.5g%. In contrast to other shock models cardiac output and perfusion pressure are not decreased by this preparation. In order to maintain arterial blood pressue above 90 mm Hg significantly more gelatine than dextran or serum has to be infused. There is no difference in hemodynamic responses after treatment with dextran or gelatine respectively. Both groups treated with colloidal solutions have the same survival rate of 38%. In the serum substituted dogs, however,survival rate is significantly greater (83%). Very likely this is due to a greater buffer capacity preventing severe acidosis, which is observed in the animals treated with colloidal solutions.", "PMID": 959132}
{"id": "pubmed23n0032_3338", "title": "[Radioimmunological estimation of cardiac glycoside concentration in the plasma].", "content": "Radioimmunoassay is a simple method for estimating the concentration of cardiac glycosides in the blood. The technique is described. An condition of the accuracy of the estimation is that the drug levels in blood and tissue have reached equilibrium, since only then will the blood concentration accurately reflect the drug level in the tissues. The therapeutic plasma levels are 0.7 to 2.0 ng/ml for digoxin and 10-30 ng/ml for digitoxin. Indications for estimating the plasma glycoside levels are reviewed; they include suspected digitalis poisoning or intolerance, interaction of glycosides with other drugs and suspected negligence on the part of the patient to take the prescribed dose.", "contents": "[Radioimmunological estimation of cardiac glycoside concentration in the plasma]. Radioimmunoassay is a simple method for estimating the concentration of cardiac glycosides in the blood. The technique is described. An condition of the accuracy of the estimation is that the drug levels in blood and tissue have reached equilibrium, since only then will the blood concentration accurately reflect the drug level in the tissues. The therapeutic plasma levels are 0.7 to 2.0 ng/ml for digoxin and 10-30 ng/ml for digitoxin. Indications for estimating the plasma glycoside levels are reviewed; they include suspected digitalis poisoning or intolerance, interaction of glycosides with other drugs and suspected negligence on the part of the patient to take the prescribed dose.", "PMID": 959134}
{"id": "pubmed23n0032_3339", "title": "[Porphyria and anesthesia. A case report and a short review (author's transl)].", "content": "The most important facts for the anesthesiologist about porphyria are presented on the basis of an own case. The acute abdomen with unusual accompanying symptoms (tachycardia, neurologic and psychic disorders) should rise suspicion of possible porphyria. The clinical diagnosis is most important for the anesthesiologist, because barbiturates may cause fatalities in 50% of the cases. Halothane should be avoided, too. Every suspected case should be carefully investigated including the family. The symptomatic therapy of the severe porphyria-attack is presented in short.", "contents": "[Porphyria and anesthesia. A case report and a short review (author's transl)]. The most important facts for the anesthesiologist about porphyria are presented on the basis of an own case. The acute abdomen with unusual accompanying symptoms (tachycardia, neurologic and psychic disorders) should rise suspicion of possible porphyria. The clinical diagnosis is most important for the anesthesiologist, because barbiturates may cause fatalities in 50% of the cases. Halothane should be avoided, too. Every suspected case should be carefully investigated including the family. The symptomatic therapy of the severe porphyria-attack is presented in short.", "PMID": 959135}
{"id": "pubmed23n0032_3340", "title": "[Radiological lung changes after thoracoabdominal surgery in the lateral decubitus position (author's transl)].", "content": "Radiological changes that may occur in the depenent lung when surgery is performed in the lateral position are generally of a transient nature; they disappear when the position of the patient is changed after the operation, but may, in some cases, be demonstrable post-operatively as atelectatic areas. From the second post-operative day onwards, and reaching a peak on the 5th post-operative day, there is an increased incidence of ventilatory disturbances and infiltrations. The course and distribution of these pulmonary disturbances point to post-operative immobilization in the recumbent position as the main causal factor.", "contents": "[Radiological lung changes after thoracoabdominal surgery in the lateral decubitus position (author's transl)]. Radiological changes that may occur in the depenent lung when surgery is performed in the lateral position are generally of a transient nature; they disappear when the position of the patient is changed after the operation, but may, in some cases, be demonstrable post-operatively as atelectatic areas. From the second post-operative day onwards, and reaching a peak on the 5th post-operative day, there is an increased incidence of ventilatory disturbances and infiltrations. The course and distribution of these pulmonary disturbances point to post-operative immobilization in the recumbent position as the main causal factor.", "PMID": 959136}
{"id": "pubmed23n0032_3341", "title": "[Surgical removal of a cerebral arterial aneurysm complicating renal failure; anaesthesiological and post-operative problems (author's transl)].", "content": "A case of cerebral arterial aneurysm associated with renal failure is reported which was treated by surgery. The anaesthesiological and post-operative problems are discussed. Surgery of the aneurysm is urgently indicated in these cases on account of the increased risk of subarachnoid haemorrhage due to heparinization during haemodialysis. Pre-operative replacement of haemodialysis by peritoneal dialysis is advisable to facilitate the treatment of cerebral oedema, maintain the electrolyte and water balance and ensure adequate post-operative enteral and parenteral feeding. The choice of anaesthetic technique, the use of controlled hypotension, the treatment of hypertensive crises, laboratory tests and the post-operative care of these cases are discussed. Experience has shown that from the anaesthesiological point of view there is no contra-indication to neurosurgery or vascular surgery in cases of renal failure provided adequate precautions are taken.", "contents": "[Surgical removal of a cerebral arterial aneurysm complicating renal failure; anaesthesiological and post-operative problems (author's transl)]. A case of cerebral arterial aneurysm associated with renal failure is reported which was treated by surgery. The anaesthesiological and post-operative problems are discussed. Surgery of the aneurysm is urgently indicated in these cases on account of the increased risk of subarachnoid haemorrhage due to heparinization during haemodialysis. Pre-operative replacement of haemodialysis by peritoneal dialysis is advisable to facilitate the treatment of cerebral oedema, maintain the electrolyte and water balance and ensure adequate post-operative enteral and parenteral feeding. The choice of anaesthetic technique, the use of controlled hypotension, the treatment of hypertensive crises, laboratory tests and the post-operative care of these cases are discussed. Experience has shown that from the anaesthesiological point of view there is no contra-indication to neurosurgery or vascular surgery in cases of renal failure provided adequate precautions are taken.", "PMID": 959138}
{"id": "pubmed23n0032_3342", "title": "[Guillain-Barre syndrome in children].", "content": "Twenty two cases of Guillian-Barr\u00e9 syndrome were studied at the Children's Hospital of the City of Morelia (State of Michoac\u00e1n, M\u00e9xico), in a four-year period; such that number represents tow out 1 000 of the patients hospitalized in that length of time. The subject of this paper is to get acquainted with the clinical features of this syndrome in this part of the country, to establish the usefulness of the study of the spinal fluid and to know the evolution of the disease in relation to the treatment with corticoids. In all of our twenty two cases, there were coincidental features with the ones reported by others; eventhough, the statistics of this syndrome in the childhood are few. The dissociation between the amounts of albumin and the count of cells in the spinal fluid confirms the diagnosis, but its abscence is not enough to exclude it. Apparently, the clinical evolution of this disease is not modified by ths use of corticoids. Finally, the most moderns etiopathogenic concepts of this syndrome are reviewed as well as its probable etiological association with the infectious mononucleosis virus (Epstein-Barr's virus).", "contents": "[Guillain-Barre syndrome in children]. Twenty two cases of Guillian-Barr\u00e9 syndrome were studied at the Children's Hospital of the City of Morelia (State of Michoac\u00e1n, M\u00e9xico), in a four-year period; such that number represents tow out 1 000 of the patients hospitalized in that length of time. The subject of this paper is to get acquainted with the clinical features of this syndrome in this part of the country, to establish the usefulness of the study of the spinal fluid and to know the evolution of the disease in relation to the treatment with corticoids. In all of our twenty two cases, there were coincidental features with the ones reported by others; eventhough, the statistics of this syndrome in the childhood are few. The dissociation between the amounts of albumin and the count of cells in the spinal fluid confirms the diagnosis, but its abscence is not enough to exclude it. Apparently, the clinical evolution of this disease is not modified by ths use of corticoids. Finally, the most moderns etiopathogenic concepts of this syndrome are reviewed as well as its probable etiological association with the infectious mononucleosis virus (Epstein-Barr's virus).", "PMID": 959169}
{"id": "pubmed23n0032_3343", "title": "Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm.", "content": "A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.", "contents": "Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm. A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.", "PMID": 959178}
{"id": "pubmed23n0032_3344", "title": "Comparison of methods for separating proteins using bisalbuminemia as a model.", "content": "Sera from bisalbuminemic chicken-turkey hybrids contain two albumins in equal amounts. These are observed as inherited electrophoretic variants and originate from the respective chicken and turkey parents. Sera from the hybrid birds served as a model system by which fractionating and indentification procedures for evaluating serum albumin variants were compared. The two albumins in the hybrid were isolated with preparative polyacrylamide gel electrophoresis (PAGE) and starch block preparative electrophoresis. Isoelectric focusing of the hybrid albumins resulted in the isolation of the turkey albumin. Interference of ampholines prevented the complete isolation of the chicken albumin. The two albumins in the hybrid have identical molecular weights and cannot be identified by sedimentation coefficient, gel filtration behavior, or sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Because of the close relatedness the chicken and turkey albumins in the hybrid cross reacted with rabbit anti-hybrid serum as well as with rabbit anti-chicken anti-turkey sera.", "contents": "Comparison of methods for separating proteins using bisalbuminemia as a model. Sera from bisalbuminemic chicken-turkey hybrids contain two albumins in equal amounts. These are observed as inherited electrophoretic variants and originate from the respective chicken and turkey parents. Sera from the hybrid birds served as a model system by which fractionating and indentification procedures for evaluating serum albumin variants were compared. The two albumins in the hybrid were isolated with preparative polyacrylamide gel electrophoresis (PAGE) and starch block preparative electrophoresis. Isoelectric focusing of the hybrid albumins resulted in the isolation of the turkey albumin. Interference of ampholines prevented the complete isolation of the chicken albumin. The two albumins in the hybrid have identical molecular weights and cannot be identified by sedimentation coefficient, gel filtration behavior, or sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Because of the close relatedness the chicken and turkey albumins in the hybrid cross reacted with rabbit anti-hybrid serum as well as with rabbit anti-chicken anti-turkey sera.", "PMID": 959177}
{"id": "pubmed23n0032_3345", "title": "Interference by divalent metals in the preparation of soluble intestinal alkaline phosphatase with n-butanal.", "content": "Certain factors were found to prevent quantitative recovery of soluble alkaline phosphatase from homogenates of chick duodenal mucosa during treatment with n-butanol. Divalent cations such as calcium, manganese and lead interfered when present at 0.1-0.2 mM. Magnesium and zinc were found to reduce enyme recovery when present at 1.0 mM during extraction. These metals had little effect on enzyme activity per se, whether added to the homogenates or enzyme extracts before dilution for assay. However, lead acetate may have a protective or activating effect on phosphatase, at 0.1-10 mM. Other factors affecting the recovery of enzyme activity after butanol solubilization are the state of dilution and pH of the homogenate and individual animal variation.", "contents": "Interference by divalent metals in the preparation of soluble intestinal alkaline phosphatase with n-butanal. Certain factors were found to prevent quantitative recovery of soluble alkaline phosphatase from homogenates of chick duodenal mucosa during treatment with n-butanol. Divalent cations such as calcium, manganese and lead interfered when present at 0.1-0.2 mM. Magnesium and zinc were found to reduce enyme recovery when present at 1.0 mM during extraction. These metals had little effect on enzyme activity per se, whether added to the homogenates or enzyme extracts before dilution for assay. However, lead acetate may have a protective or activating effect on phosphatase, at 0.1-10 mM. Other factors affecting the recovery of enzyme activity after butanol solubilization are the state of dilution and pH of the homogenate and individual animal variation.", "PMID": 959179}
{"id": "pubmed23n0032_3346", "title": "The binding of asparagine, glutamine and homoserine to human erythrocytes containing hemoglobins S and CS and to soluble hemoglobins S and CS.", "content": "Tritium labeled asparagine binds to oxyhemoglobin S and to a mixture of hemoglobins C and S in the molar ratio of 3.38:1 and 8.2:1 respectively. From the dialysis equilibrium studies it appears that labeled asparagine does not bind to oxy- or deoxy- hemoglobin A nor to deoxyhemoglobin S. The constant for equilibrium association of asparagine for oxyhemoglobin S is 7.38 x 10(7) M(-1) and for oxyhemoglobin CS 4.8 X 10(4) M(-1) at 23 degrees C. Tritium labeled asparagine is bound to oxyhemoglobin S and CS sufficiently strongly to prevent dissociation under the conditions of gel electrophoresis at pH 9.50. The protein with and without bound asparagine, glutamine or homoserine, is indistinguishable in molecular net charge and size by the criteria of quantitative polyacrylamide gel electrophoresis (PAGE). Also there were no significant differences in mobility between hemoglobin S and hemoglobin C in the presence and absence of asparagine, glutamine and homoserine as detectable in agar coated cellulose acetate electrophoresis at pH 6.3.", "contents": "The binding of asparagine, glutamine and homoserine to human erythrocytes containing hemoglobins S and CS and to soluble hemoglobins S and CS. Tritium labeled asparagine binds to oxyhemoglobin S and to a mixture of hemoglobins C and S in the molar ratio of 3.38:1 and 8.2:1 respectively. From the dialysis equilibrium studies it appears that labeled asparagine does not bind to oxy- or deoxy- hemoglobin A nor to deoxyhemoglobin S. The constant for equilibrium association of asparagine for oxyhemoglobin S is 7.38 x 10(7) M(-1) and for oxyhemoglobin CS 4.8 X 10(4) M(-1) at 23 degrees C. Tritium labeled asparagine is bound to oxyhemoglobin S and CS sufficiently strongly to prevent dissociation under the conditions of gel electrophoresis at pH 9.50. The protein with and without bound asparagine, glutamine or homoserine, is indistinguishable in molecular net charge and size by the criteria of quantitative polyacrylamide gel electrophoresis (PAGE). Also there were no significant differences in mobility between hemoglobin S and hemoglobin C in the presence and absence of asparagine, glutamine and homoserine as detectable in agar coated cellulose acetate electrophoresis at pH 6.3.", "PMID": 959181}
{"id": "pubmed23n0032_3347", "title": "[Diabetes mellitus, arterial hypertension and serum levels of renin].", "content": "Plasmatic renin levels were measured in 17 adults patients with diagnosis of diabetes mellitus and systemic arterial hypertension. None of them was under antihypertensive therapy. The results obtained showed lessening of renin levels in 13 cases, normal levels in two cases, no valuable results in one and Significative increase in other. Renal biopsy was performed in 8 patients and in all cases nephropathies were demonstrated; only one of them had normal renin levels. We have concluded that more than 75% of our cases were hyporeninemic hypertensive patients. Our findings are according with other medical papers that have been published before. Finally, we try to explain the mechanisms upon which theorethical causes are based.", "contents": "[Diabetes mellitus, arterial hypertension and serum levels of renin]. Plasmatic renin levels were measured in 17 adults patients with diagnosis of diabetes mellitus and systemic arterial hypertension. None of them was under antihypertensive therapy. The results obtained showed lessening of renin levels in 13 cases, normal levels in two cases, no valuable results in one and Significative increase in other. Renal biopsy was performed in 8 patients and in all cases nephropathies were demonstrated; only one of them had normal renin levels. We have concluded that more than 75% of our cases were hyporeninemic hypertensive patients. Our findings are according with other medical papers that have been published before. Finally, we try to explain the mechanisms upon which theorethical causes are based.", "PMID": 959174}
{"id": "pubmed23n0032_3348", "title": "[Serum lipids and electrophoresis of lipoproteins in the normal newborn infant].", "content": "The levels of plasma lipids and the values of the different electrophoretic fractions of lipoproteins were measured in cord blood of 300 new borns selected at random. The value found are expressed as me +/- S.D. (limits X +/-2s) and were the following: total cholesterol 80.4 +/- 17.5 (limits 45-115) mg./100 ml., LDL cholesterol (by precipitation) 33+/-11 (limits 11-55) mg./100 ml., betalipo-protein (by immunodifusion) 142.7+/-26.4 (limits 90-195) mg./100 ml. and triglycerides 35+/-13 (limits 9-61) mg./100 ml. The figures for the different electrophoretic fractions of lipoproteins were expressed in per cent of the total area and were the following: betalipoproteins 42.9+/-10.3 (limits 22-63%), prebetalipoproteins 3.09+/-2.79 (limits 0-8.6%) and alphalipoproteins 53.8 +/- 9.5 (limits 35-73%). The beta/alpha ratio was 0.91+/-0.33 (limits 0.25-1.6).", "contents": "[Serum lipids and electrophoresis of lipoproteins in the normal newborn infant]. The levels of plasma lipids and the values of the different electrophoretic fractions of lipoproteins were measured in cord blood of 300 new borns selected at random. The value found are expressed as me +/- S.D. (limits X +/-2s) and were the following: total cholesterol 80.4 +/- 17.5 (limits 45-115) mg./100 ml., LDL cholesterol (by precipitation) 33+/-11 (limits 11-55) mg./100 ml., betalipo-protein (by immunodifusion) 142.7+/-26.4 (limits 90-195) mg./100 ml. and triglycerides 35+/-13 (limits 9-61) mg./100 ml. The figures for the different electrophoretic fractions of lipoproteins were expressed in per cent of the total area and were the following: betalipoproteins 42.9+/-10.3 (limits 22-63%), prebetalipoproteins 3.09+/-2.79 (limits 0-8.6%) and alphalipoproteins 53.8 +/- 9.5 (limits 35-73%). The beta/alpha ratio was 0.91+/-0.33 (limits 0.25-1.6).", "PMID": 959176}
{"id": "pubmed23n0032_3349", "title": "Management of metastases to the lymph glands of the neck.", "content": "The survival rate of matched pairs of patients, one patient in each pair being submitted to prophylactic neck dissection, the other to a policy of \"wait and see\" has been measured: there was no significant difference in the survival rates of the two groups. Surgery for most patients with a gland in the neck gives better results than radiotherapy, and can produce satisfactory results with such procedures as supraglottic laryngectomy to epiglottic tumours and replacement with the deltopectoral flap for tonsillar tumours. On the other hand, surgery is probably contraindicated for patients with antral carcinoma and a gland in the neck, whom it rarely cures. Surgery does not increase the survival of patients with bilateral glands in the neck (except those with supraglottic tumours) and its contraindicated. Surgery can prolong the survival of patients with nodes fixed to the skin, the mandible or the external carotid artery, and may occassionally cure such patients.", "contents": "Management of metastases to the lymph glands of the neck. The survival rate of matched pairs of patients, one patient in each pair being submitted to prophylactic neck dissection, the other to a policy of \"wait and see\" has been measured: there was no significant difference in the survival rates of the two groups. Surgery for most patients with a gland in the neck gives better results than radiotherapy, and can produce satisfactory results with such procedures as supraglottic laryngectomy to epiglottic tumours and replacement with the deltopectoral flap for tonsillar tumours. On the other hand, surgery is probably contraindicated for patients with antral carcinoma and a gland in the neck, whom it rarely cures. Surgery does not increase the survival of patients with bilateral glands in the neck (except those with supraglottic tumours) and its contraindicated. Surgery can prolong the survival of patients with nodes fixed to the skin, the mandible or the external carotid artery, and may occassionally cure such patients.", "PMID": 959210}
{"id": "pubmed23n0032_3350", "title": "The epidemiology of disasters.", "content": "Over the last few years there  has been an increasing awareness that some kind of disaster management should be possible. The emphasis is now moving from post-disaster improvisation to predisaster preparedness. The League of Red Cross Societies has increasingly encouraged predisaster planning in countries at risk. A new United Nations agency - United Nations Disaster Relief Office (UNDRO)- has been set up with headquarters in Geneva. Coordination and exchange of information between agencies engaged in disaster relief are becoming the rule rather than the exception, and a number of groups have started with the specific objective of making professional expertise available to disaster management. A number of private initiatives have been taken, meetings have been organized, research centers set up, and research projects launched. The study of disasters needs to be approached on a multidisciplinary basis, the more so since the health component is only one part of the broad disaster problem and, perhaps not the major one. Social scientists, psychologists, administrators, economists, geographers, have been or are conducting a number of studies on natural disasters. These studies have provided new insights and have proved most useful in preparing for disasters and increasing the effectiveness and acceptance of relief operations. This is a vital and challenging field, wide open for research. It is now time for epidemiologists and community health scientists to enter the fray and provide much needed information on which a rational, effective and flexible policy for the management of disasters can be based.", "contents": "The epidemiology of disasters. Over the last few years there  has been an increasing awareness that some kind of disaster management should be possible. The emphasis is now moving from post-disaster improvisation to predisaster preparedness. The League of Red Cross Societies has increasingly encouraged predisaster planning in countries at risk. A new United Nations agency - United Nations Disaster Relief Office (UNDRO)- has been set up with headquarters in Geneva. Coordination and exchange of information between agencies engaged in disaster relief are becoming the rule rather than the exception, and a number of groups have started with the specific objective of making professional expertise available to disaster management. A number of private initiatives have been taken, meetings have been organized, research centers set up, and research projects launched. The study of disasters needs to be approached on a multidisciplinary basis, the more so since the health component is only one part of the broad disaster problem and, perhaps not the major one. Social scientists, psychologists, administrators, economists, geographers, have been or are conducting a number of studies on natural disasters. These studies have provided new insights and have proved most useful in preparing for disasters and increasing the effectiveness and acceptance of relief operations. This is a vital and challenging field, wide open for research. It is now time for epidemiologists and community health scientists to enter the fray and provide much needed information on which a rational, effective and flexible policy for the management of disasters can be based.", "PMID": 959212}
{"id": "pubmed23n0032_3351", "title": "Immune responses to mycobacteria in mice and men.", "content": "This paper contains discussion of a number of mechanisms which may explain the suppression of the cell-mediated immune response during the phase of dissemination of mycobacterial infection in mice and men. However, it is not yet possible to evaluate the relative importance of these mechanisms, or even to say which are the causes and which are the consequences of that disseminaton.", "contents": "Immune responses to mycobacteria in mice and men. This paper contains discussion of a number of mechanisms which may explain the suppression of the cell-mediated immune response during the phase of dissemination of mycobacterial infection in mice and men. However, it is not yet possible to evaluate the relative importance of these mechanisms, or even to say which are the causes and which are the consequences of that disseminaton.", "PMID": 959218}
{"id": "pubmed23n0032_3352", "title": "Synthesis of some quinazolone derivatives structurally related to certain sedatives and hypnotics.", "content": "The preparation of certain 3-substituted derivatives of 3.4-dihydro-2-methyl-4-oxoquinazoline is described. The synthesis fo a quinazolone-barbituric acid derivative, in which the two constituting moieties are separated by methylene groups, was achieved by reacting 3-[B-haloethyl]-3.4-dihydro-2-methyl-4-oxoquinazoline with sodio malonic ester and subsequent cyclization of the ester so formed with urea. Attempts to introduce the alpha-glutaryl group at the position 3 of the quinazolone ring system via different routes were unsuccessful.", "contents": "Synthesis of some quinazolone derivatives structurally related to certain sedatives and hypnotics. The preparation of certain 3-substituted derivatives of 3.4-dihydro-2-methyl-4-oxoquinazoline is described. The synthesis fo a quinazolone-barbituric acid derivative, in which the two constituting moieties are separated by methylene groups, was achieved by reacting 3-[B-haloethyl]-3.4-dihydro-2-methyl-4-oxoquinazoline with sodio malonic ester and subsequent cyclization of the ester so formed with urea. Attempts to introduce the alpha-glutaryl group at the position 3 of the quinazolone ring system via different routes were unsuccessful.", "PMID": 959265}
{"id": "pubmed23n0032_3353", "title": "The application of ferrihydroxamic complexes to photocolorimetric determination of drugs. Part 2: Quantitative determination of calcium pangamate (vitamin B15) and its calcium gluconate mixtures.", "content": "A quantitative method for calcium pangamate (Vitamin B15) and calcium gluconate determination, separately and in mixture, is proposed on the basis of the ferric hydroxamate procedure. The method may also be applied to the determination separately and in mixture, is proposed on the basis of the ferric of both preparations in mixture with various other substances which are not converted into lactone from in acid medium, and which the ferric hydroxamate procedure fails to develop.", "contents": "The application of ferrihydroxamic complexes to photocolorimetric determination of drugs. Part 2: Quantitative determination of calcium pangamate (vitamin B15) and its calcium gluconate mixtures. A quantitative method for calcium pangamate (Vitamin B15) and calcium gluconate determination, separately and in mixture, is proposed on the basis of the ferric hydroxamate procedure. The method may also be applied to the determination separately and in mixture, is proposed on the basis of the ferric of both preparations in mixture with various other substances which are not converted into lactone from in acid medium, and which the ferric hydroxamate procedure fails to develop.", "PMID": 959268}
{"id": "pubmed23n0032_3354", "title": "The cyclodesulphurization of thio-compounds. Part 12: A new simple method for the synthesis of N-alpha-substituted benzimidazoles from thiourea derivatives.", "content": "A new facile method has been established for the synthesis of N-alpha-substituted benzimidazoles by cyclodesulphurization of various N-[2-amino]-phenyl-N'-alkyl, -aryl and -aralkylthiourea derivatives with mercuric salts. Besides mercuric chloride, the use of mercuric oxide and mercuric acetate was successful for the first time in effecting ring closure of thio-compounds. The attempted use of acid condensing agents showed phosphoryl chloride to be less efficient than mercuric salts in conducting the cyclization reactions and led to poor yields of cyclized products.", "contents": "The cyclodesulphurization of thio-compounds. Part 12: A new simple method for the synthesis of N-alpha-substituted benzimidazoles from thiourea derivatives. A new facile method has been established for the synthesis of N-alpha-substituted benzimidazoles by cyclodesulphurization of various N-[2-amino]-phenyl-N'-alkyl, -aryl and -aralkylthiourea derivatives with mercuric salts. Besides mercuric chloride, the use of mercuric oxide and mercuric acetate was successful for the first time in effecting ring closure of thio-compounds. The attempted use of acid condensing agents showed phosphoryl chloride to be less efficient than mercuric salts in conducting the cyclization reactions and led to poor yields of cyclized products.", "PMID": 959294}
{"id": "pubmed23n0032_3355", "title": "Spectrophotometric determination of certain volatile oils. Part 1: Assay for phenols in volatile oils of clove and thyme.", "content": "A differential spectrophotometric method was described for the assay of thyme and clove oils. The method is based on the relative absorbance measurement of the thymate or eugenate anions to the respective molecular thymol or eugenol in alcoholic medium. The results of the differential method, compared with those of the conventional method, are more accurate.", "contents": "Spectrophotometric determination of certain volatile oils. Part 1: Assay for phenols in volatile oils of clove and thyme. A differential spectrophotometric method was described for the assay of thyme and clove oils. The method is based on the relative absorbance measurement of the thymate or eugenate anions to the respective molecular thymol or eugenol in alcoholic medium. The results of the differential method, compared with those of the conventional method, are more accurate.", "PMID": 959296}
{"id": "pubmed23n0032_3356", "title": "A preliminary study of the physical stability of tablets prepared from powders stored under tropical conditions.", "content": "A study has been made of the effects of storing powders under tropical conditions before compression on the physical stability of the tablets prepared from them, as measured by changes in tablet weight, volume and hardness. Significant changes in stability have been observed and these have been related to the different degrees of moisture uptake by the powders during storage. In addition, the initial powder storage conditions have been shown to be important when considering the best method of tablet manufacture with respect to tablet stability.", "contents": "A preliminary study of the physical stability of tablets prepared from powders stored under tropical conditions. A study has been made of the effects of storing powders under tropical conditions before compression on the physical stability of the tablets prepared from them, as measured by changes in tablet weight, volume and hardness. Significant changes in stability have been observed and these have been related to the different degrees of moisture uptake by the powders during storage. In addition, the initial powder storage conditions have been shown to be important when considering the best method of tablet manufacture with respect to tablet stability.", "PMID": 959298}
{"id": "pubmed23n0032_3357", "title": "Influence of phosphoserine on the performance of rats on the rotarod.", "content": "The anti-fatigue effect of phosphoserine, L-serine and a mixture of sodium phosphate and L-serine has been studied in rats. Phosphoserine orally administered induced a statistically significant anti-fatigue effect in rats of both sexes submitted to a training test by rotarod, with 14 sessions in 3 days. Furthermore, phosphoserine was utilized better than the mixture of sodium phosphate and L-serine, whereas L-serine alone induced no effect. Therefore the effect of phosphoserine seems to be due to the phosphoryl bond.", "contents": "Influence of phosphoserine on the performance of rats on the rotarod. The anti-fatigue effect of phosphoserine, L-serine and a mixture of sodium phosphate and L-serine has been studied in rats. Phosphoserine orally administered induced a statistically significant anti-fatigue effect in rats of both sexes submitted to a training test by rotarod, with 14 sessions in 3 days. Furthermore, phosphoserine was utilized better than the mixture of sodium phosphate and L-serine, whereas L-serine alone induced no effect. Therefore the effect of phosphoserine seems to be due to the phosphoryl bond.", "PMID": 959308}
{"id": "pubmed23n0032_3358", "title": "Effects of caffeine and ethanol on the blood-brain barrier in rats.", "content": "In rats, pretreatment with caffeine or ethanol shortens the duration of the harmine tremor and decreases the brain concentrations of this alkaloid. Caffeine and ethanol do not influence the plasma concentration of harmine, its plasma protein binding and its cerebral concentration at the termination of the tremor. The findings are regarded as indicative of a reduction by caffeine and ethanol of the blood-brain barrier permeability to harmine.", "contents": "Effects of caffeine and ethanol on the blood-brain barrier in rats. In rats, pretreatment with caffeine or ethanol shortens the duration of the harmine tremor and decreases the brain concentrations of this alkaloid. Caffeine and ethanol do not influence the plasma concentration of harmine, its plasma protein binding and its cerebral concentration at the termination of the tremor. The findings are regarded as indicative of a reduction by caffeine and ethanol of the blood-brain barrier permeability to harmine.", "PMID": 959309}
{"id": "pubmed23n0032_3359", "title": "The structure-action relationship and kinetics of some naloxone and naltrexone derivatives.", "content": "The isolated longitudinal muscle preparation (with attached Auerbach plexus) of the guinea pig ileum was used to investigate the structure activity relationship and kinetics of some naloxone and naltrexone derivatives and that of cyclazocine. The agonist used for the investigation of the antagonistic effect of these compounds was 6-azidomorphine (AM). AM was found to be an about 20 times more potent agonist than morphine. In contrast to cyclazocine, which also was found to be approximately 15 times more potent agonist than morphine, naloxone had no demonstrable agonistic activity and naltrexone and the various naloxone and naltrexone derivatives had only insignificant agonistic activity with ED50/Ke ratios ranging from 2,000 to about 120,000. All compounds tested were competitive reversible antagonists of AM. 6-Methylene substitution caused an approximate 50 and 100% increase of the antagonistic activity of naloxone and naltrexone, respectively, and decreased the duration of action of naloxone. 3-Acetate or 3-nicotinate substitution decreased potency and had no effect on the duration of naloxone action. There is a correlation between tachyphylaxis observed on the inhibition of longitudinal muscle contraction and antagonist activity of narcotic agonists.", "contents": "The structure-action relationship and kinetics of some naloxone and naltrexone derivatives. The isolated longitudinal muscle preparation (with attached Auerbach plexus) of the guinea pig ileum was used to investigate the structure activity relationship and kinetics of some naloxone and naltrexone derivatives and that of cyclazocine. The agonist used for the investigation of the antagonistic effect of these compounds was 6-azidomorphine (AM). AM was found to be an about 20 times more potent agonist than morphine. In contrast to cyclazocine, which also was found to be approximately 15 times more potent agonist than morphine, naloxone had no demonstrable agonistic activity and naltrexone and the various naloxone and naltrexone derivatives had only insignificant agonistic activity with ED50/Ke ratios ranging from 2,000 to about 120,000. All compounds tested were competitive reversible antagonists of AM. 6-Methylene substitution caused an approximate 50 and 100% increase of the antagonistic activity of naloxone and naltrexone, respectively, and decreased the duration of action of naloxone. 3-Acetate or 3-nicotinate substitution decreased potency and had no effect on the duration of naloxone action. There is a correlation between tachyphylaxis observed on the inhibition of longitudinal muscle contraction and antagonist activity of narcotic agonists.", "PMID": 959310}
{"id": "pubmed23n0032_3360", "title": "Effect of bombesin, a natural tetradecapeptide, on myoelectrical and mechanical activity of isolated, ex vivo perfused, canine stomach.", "content": "Electrical and mechanical activity were recorded from the canine stomach, isolated and ex vivo perfused with homologous blood. The action of Bombesin, a natural tetradecapeptide, was compared with the action of pentagastrin, methacholine and electrical stimulation on the electrical and mechanical activity of this isolated preparation. All drugs used and vagal stimulation resulted in premature control potentials, uncoupling of the normal phase locked pattern of electrical control activity, appearance of response activity and appearance of mechanical reaction. These responses were blocked or decreased by atropine, tetrodotoxin and hexamethonium. It appears that the neural release of acetylcholine is implicated in the mechanism of action of Bombesin gastrointestinal motility.", "contents": "Effect of bombesin, a natural tetradecapeptide, on myoelectrical and mechanical activity of isolated, ex vivo perfused, canine stomach. Electrical and mechanical activity were recorded from the canine stomach, isolated and ex vivo perfused with homologous blood. The action of Bombesin, a natural tetradecapeptide, was compared with the action of pentagastrin, methacholine and electrical stimulation on the electrical and mechanical activity of this isolated preparation. All drugs used and vagal stimulation resulted in premature control potentials, uncoupling of the normal phase locked pattern of electrical control activity, appearance of response activity and appearance of mechanical reaction. These responses were blocked or decreased by atropine, tetrodotoxin and hexamethonium. It appears that the neural release of acetylcholine is implicated in the mechanism of action of Bombesin gastrointestinal motility.", "PMID": 959311}
{"id": "pubmed23n0032_3361", "title": "In vivo and in vitro studies on the effect of tetrahydropapaveroline and salsolinol on COMT and MAO activity in rat brain.", "content": "Studies were made on COMT and MAO activity in rats 2, 4, 6, 12 and 24 h after intraperitoneal treatment with 8 and 60 mg/kg doses of tetrahydropapaveroline (THP), and 10 and 20 days after treatment with 8 mg/kg/day. Results suggested COMT activity inhibition only after 2 h of treatment. Experiments were also performed on THP and salsolinol methylation capacity in vitro. Results showed that both alkaloids use COMT enzymes for methylation and can also act as competitive inhibitors of COMT and MAO activity.", "contents": "In vivo and in vitro studies on the effect of tetrahydropapaveroline and salsolinol on COMT and MAO activity in rat brain. Studies were made on COMT and MAO activity in rats 2, 4, 6, 12 and 24 h after intraperitoneal treatment with 8 and 60 mg/kg doses of tetrahydropapaveroline (THP), and 10 and 20 days after treatment with 8 mg/kg/day. Results suggested COMT activity inhibition only after 2 h of treatment. Experiments were also performed on THP and salsolinol methylation capacity in vitro. Results showed that both alkaloids use COMT enzymes for methylation and can also act as competitive inhibitors of COMT and MAO activity.", "PMID": 959312}
{"id": "pubmed23n0032_3362", "title": "Effect of glucagon on myoelectrical and mechnical activity of the isolated homologous perfused porcine stomach.", "content": "Totally isolated whole porcine stomachs, perfused extracorporeally with homologous blood, were used for recording gastric electrical and mechanical activity. Hormones Hormones were administered into the arterial system of the isolated organ. Pentagastrin, methacholine and electrical stimulation of the vagus nerve induced premature control potentials with uncoupling of the normal, phase-locked, pattern of electrical control activity and also produced electrical response activity and mechanical reaction. When glucagon was infused a progressive decrease of frequency of the cycles of electrical control activity was observed. Glucagon also abolished electrical response activity and mechanical activity, if they were present prior to hormone infusion. When pentagastrin, methacholine or electrical stimulation were applied during the infusion of glucagon, the responses to these stimulants were decreased or absent. The inhibitory action of glucagon was dependent on the duration of infusion of this hormone and lasted for 30-45 min after the infusion was discontinued. During infusion of glucagon the mean gastric arterial perfusion pressure and gastric peripheral vascular resistance were reduced.", "contents": "Effect of glucagon on myoelectrical and mechnical activity of the isolated homologous perfused porcine stomach. Totally isolated whole porcine stomachs, perfused extracorporeally with homologous blood, were used for recording gastric electrical and mechanical activity. Hormones Hormones were administered into the arterial system of the isolated organ. Pentagastrin, methacholine and electrical stimulation of the vagus nerve induced premature control potentials with uncoupling of the normal, phase-locked, pattern of electrical control activity and also produced electrical response activity and mechanical reaction. When glucagon was infused a progressive decrease of frequency of the cycles of electrical control activity was observed. Glucagon also abolished electrical response activity and mechanical activity, if they were present prior to hormone infusion. When pentagastrin, methacholine or electrical stimulation were applied during the infusion of glucagon, the responses to these stimulants were decreased or absent. The inhibitory action of glucagon was dependent on the duration of infusion of this hormone and lasted for 30-45 min after the infusion was discontinued. During infusion of glucagon the mean gastric arterial perfusion pressure and gastric peripheral vascular resistance were reduced.", "PMID": 959313}
{"id": "pubmed23n0032_3363", "title": "Effects of diphenhydramine, naphazoline and m-amino-alpha(1-aminoethyl)benzyl alcohol dihydrochloride on the nasal mucosa determined by impedance method: a simple method for evaluation of nasal decongestant.", "content": "A simple, new method was devised for evaluating nasal decongestants. In anesthetized dogs, two needle electrodes were inserted bilaterally into the superficial mucosa of the nasal wings. An impedance plethysmorgraphy provided with a DC output was used to measure impedance between the two electrodes. When 1% histamine solution was sprayed into a nostril focusing on the inside mucosa of a nasal wing, impedance decreased markedly and thereafter recovered to a control level within 1-1.5 h. Comparable responses were obtained when the same solution was sprayed into the opposite nostril. The drugs to be tested were administered intravenously or topically between these two histamine application. Intravenous administrations of diphenhydramine (0.5 mg/kg) and m-amino-alpha)1-aminoethyl) benzyl alcohol dihydrochloride (0.1 mg/kg) inhibited the histamine effect completely. Pretreatment with naphazoline administered topically also inhibited impedance changes cause by histamine application. Local appliations of acetycholine (10%) and bradykinin (0.1%) did not change nasal impedance significantly in any instances.", "contents": "Effects of diphenhydramine, naphazoline and m-amino-alpha(1-aminoethyl)benzyl alcohol dihydrochloride on the nasal mucosa determined by impedance method: a simple method for evaluation of nasal decongestant. A simple, new method was devised for evaluating nasal decongestants. In anesthetized dogs, two needle electrodes were inserted bilaterally into the superficial mucosa of the nasal wings. An impedance plethysmorgraphy provided with a DC output was used to measure impedance between the two electrodes. When 1% histamine solution was sprayed into a nostril focusing on the inside mucosa of a nasal wing, impedance decreased markedly and thereafter recovered to a control level within 1-1.5 h. Comparable responses were obtained when the same solution was sprayed into the opposite nostril. The drugs to be tested were administered intravenously or topically between these two histamine application. Intravenous administrations of diphenhydramine (0.5 mg/kg) and m-amino-alpha)1-aminoethyl) benzyl alcohol dihydrochloride (0.1 mg/kg) inhibited the histamine effect completely. Pretreatment with naphazoline administered topically also inhibited impedance changes cause by histamine application. Local appliations of acetycholine (10%) and bradykinin (0.1%) did not change nasal impedance significantly in any instances.", "PMID": 959314}
{"id": "pubmed23n0032_3364", "title": "Effects of ethanol on compound action potential and refractory periods of toad sciatic nerve.", "content": "The action of ethanol on compound action potential, refractory periods and chronaxia are analyzed in 28 isolated sciatic nerves of toads. 85 mM ethanol increases the compound action potential and decreases the refractory periods. 342 and 513 mM ehtanol decrease the compound action potential and increase the absolute and relative refractory periods. These biphasic effects are discussed in relation with changes in Na+ and K+ permeabilities and to the greater sensitivity of thin fibers to the chemicals.", "contents": "Effects of ethanol on compound action potential and refractory periods of toad sciatic nerve. The action of ethanol on compound action potential, refractory periods and chronaxia are analyzed in 28 isolated sciatic nerves of toads. 85 mM ethanol increases the compound action potential and decreases the refractory periods. 342 and 513 mM ehtanol decrease the compound action potential and increase the absolute and relative refractory periods. These biphasic effects are discussed in relation with changes in Na+ and K+ permeabilities and to the greater sensitivity of thin fibers to the chemicals.", "PMID": 959315}
{"id": "pubmed23n0032_3365", "title": "Alkali metal ions and ethanol narcosis in mice.", "content": "The effect of an equimolar dose of LiCl, RbCl or CsCl on the depressant action of ethanol (ETOH) was studied as a function of duration of ETOH-mediated narcosis in mice. The alkali metal ions were administered acutely or semi-chronically prior to the administration of a narcotic dose of ETOH. Semi-chronic treatment with RbCl or CsCl resulted in decreased duration of ETOH-mediated narcosis, which was persistent for 5 days after discontinued administration of RbCl and CsCl. Conversely, a prolongation of ETOH-produced narcosis in Li-treated mice occured only when ETOH was shortly administered after sub-chronic regiments of LiCl. Semi-chronic treatment with an equimolar dose of LiCl, RbCl or CsCl produced little changes in the specific activities of liver alcohol and aldehyde dehydrogenase. It is concluded that both Rb+ and Cs+ possess antidepressant properties and might be useful agents in negating the depressant action of ETOH and/or some of its toxic manifestations.", "contents": "Alkali metal ions and ethanol narcosis in mice. The effect of an equimolar dose of LiCl, RbCl or CsCl on the depressant action of ethanol (ETOH) was studied as a function of duration of ETOH-mediated narcosis in mice. The alkali metal ions were administered acutely or semi-chronically prior to the administration of a narcotic dose of ETOH. Semi-chronic treatment with RbCl or CsCl resulted in decreased duration of ETOH-mediated narcosis, which was persistent for 5 days after discontinued administration of RbCl and CsCl. Conversely, a prolongation of ETOH-produced narcosis in Li-treated mice occured only when ETOH was shortly administered after sub-chronic regiments of LiCl. Semi-chronic treatment with an equimolar dose of LiCl, RbCl or CsCl produced little changes in the specific activities of liver alcohol and aldehyde dehydrogenase. It is concluded that both Rb+ and Cs+ possess antidepressant properties and might be useful agents in negating the depressant action of ETOH and/or some of its toxic manifestations.", "PMID": 959316}
{"id": "pubmed23n0032_3366", "title": "Relative lipolytic activities of selected catecholamines in the mouse.", "content": "The adipokinetic activities, relative to isoproterenol as 1,000, in sedated, phenoxybenzamine pretreated unfasted mice were: norepinephrine 540, epinephrine 330, N-t-butylnorepinephrine 130, isoetharine 30 and N-cyclopentylbutanefrine 10. These relative activities correlate (r=0.88) with the medians of some previously defined beta1-adrenoceptor activities, but not (r=less than 0.1) with the medians of some previously defined beta2-adrenoceptor activities. These findings are in accord with the previously suggested view that an alpha, beta1-, beta2-, adrenoceptor concept is in better accord with experimental evidence that is an alpha- and beta-adrenoceptor one.", "contents": "Relative lipolytic activities of selected catecholamines in the mouse. The adipokinetic activities, relative to isoproterenol as 1,000, in sedated, phenoxybenzamine pretreated unfasted mice were: norepinephrine 540, epinephrine 330, N-t-butylnorepinephrine 130, isoetharine 30 and N-cyclopentylbutanefrine 10. These relative activities correlate (r=0.88) with the medians of some previously defined beta1-adrenoceptor activities, but not (r=less than 0.1) with the medians of some previously defined beta2-adrenoceptor activities. These findings are in accord with the previously suggested view that an alpha, beta1-, beta2-, adrenoceptor concept is in better accord with experimental evidence that is an alpha- and beta-adrenoceptor one.", "PMID": 959317}
{"id": "pubmed23n0032_3367", "title": "Splanchnic vascular responses to the infusion of prostaglandins A1, A2 and B1.", "content": "The effects of PGA1, PGA2 and PGB1 on the vasculature of the liver and small intestine were studied in 73 dogs. Infusions were made into a branch of the superior mesenteric artery, the hepatic artery, portal vein or femoral vein. They decreased systemic arterial pressure and dilated the hepatic arterial and prehepatic splanchnic (small intestinal) vascular beds, PGA being most active. Dilator response was not decreased by beta-adrenergic blockade. Compounds appear to be inactivated by liver and decreased systemic pressure less when infused directly into liver circulation. Dilator response was transient, particulary in small intestine, and abated or even converted to constriction when infusion was continued for a period of time. Intrahepatic portal venous vasculature appeared to be constricted by PGA.", "contents": "Splanchnic vascular responses to the infusion of prostaglandins A1, A2 and B1. The effects of PGA1, PGA2 and PGB1 on the vasculature of the liver and small intestine were studied in 73 dogs. Infusions were made into a branch of the superior mesenteric artery, the hepatic artery, portal vein or femoral vein. They decreased systemic arterial pressure and dilated the hepatic arterial and prehepatic splanchnic (small intestinal) vascular beds, PGA being most active. Dilator response was not decreased by beta-adrenergic blockade. Compounds appear to be inactivated by liver and decreased systemic pressure less when infused directly into liver circulation. Dilator response was transient, particulary in small intestine, and abated or even converted to constriction when infusion was continued for a period of time. Intrahepatic portal venous vasculature appeared to be constricted by PGA.", "PMID": 959318}
{"id": "pubmed23n0032_3368", "title": "Assessment of methodology in single-dose studies of digoxin bioavailability.", "content": "Eight healthy males received 0.75 mg of digoxin by ten modes of administration in a single-dose multicrossover bioavilability study. Digoxin concentration in multiple blood samples drawn after each dose and in six consecutive 24-hour urine collections were used to calculate the areas under the 4-, 8-, and 24-hour serum concentration curve (A-4, A-8, A-24), and the excretion of digoxin during 1 day (U-1) and 6 days (U-6) following each dose. All five methods of assessment gave very similar information on bioavailability. Individual values of A-4 and A-8 were highly correlated (r=0.973) and had similar variability. A-24 was more variable than A-4 and A-8, and was not as well correlated with either. U1- and U-6 were highly correlated (r=0.944), and had nearly identical variability which was less than that of any of the area measures. Thus, urinary excreation data provides more reliable and reproducible information about completeness of absorption of digoxin than data based upon serum concentrations. Extending the period of urine collection beyond 1 day or the blood sampling period beyond 4 or 8 h does not enhance the reliability or usefulness of digoxin bioavailability studies.", "contents": "Assessment of methodology in single-dose studies of digoxin bioavailability. Eight healthy males received 0.75 mg of digoxin by ten modes of administration in a single-dose multicrossover bioavilability study. Digoxin concentration in multiple blood samples drawn after each dose and in six consecutive 24-hour urine collections were used to calculate the areas under the 4-, 8-, and 24-hour serum concentration curve (A-4, A-8, A-24), and the excretion of digoxin during 1 day (U-1) and 6 days (U-6) following each dose. All five methods of assessment gave very similar information on bioavailability. Individual values of A-4 and A-8 were highly correlated (r=0.973) and had similar variability. A-24 was more variable than A-4 and A-8, and was not as well correlated with either. U1- and U-6 were highly correlated (r=0.944), and had nearly identical variability which was less than that of any of the area measures. Thus, urinary excreation data provides more reliable and reproducible information about completeness of absorption of digoxin than data based upon serum concentrations. Extending the period of urine collection beyond 1 day or the blood sampling period beyond 4 or 8 h does not enhance the reliability or usefulness of digoxin bioavailability studies.", "PMID": 959319}
{"id": "pubmed23n0032_3369", "title": "Electrophysiological studies of 6-(N,N-diethylamino)-Hexyl-3,4,5,-Trimethoxybenzoate on ventricular muscle and conduction system.", "content": "The agent, 6-(N,N-diethylamino)-hexyl- 3, 4, 5,-Trimethoxybenzoate (TMB-6) which relaxes smooth and skeletal muscle by interfering with intracellular Ca ++ availability was tested for its electrophysiological effects on canine cardiac Purkinje fibres and feline papillary muscles. In both tissues the drug causes a decrease in resting membrane potential and action potential amplitude as well as spontaneous rate in Purkinje fibres, and an increase in stimulus needed to reach threshold. The minimum effective dose for both tissues was similar at 7.32 x 10-5 M. The drug effects were reversible in both tissues upon removal of the TMB-6. The Ca++ -dependent action potentials of canine cardiac Purkinje fibres were also inhibited by TMB-6 at dose range of 7.32 x 10-5 -24.4 x 10-5 M. Because of its electro physiological effects of threshold, automaticity and \"slow response\" action potentials and its reversibility, TMB-6 could become a useful antiarrhythmic drug.", "contents": "Electrophysiological studies of 6-(N,N-diethylamino)-Hexyl-3,4,5,-Trimethoxybenzoate on ventricular muscle and conduction system. The agent, 6-(N,N-diethylamino)-hexyl- 3, 4, 5,-Trimethoxybenzoate (TMB-6) which relaxes smooth and skeletal muscle by interfering with intracellular Ca ++ availability was tested for its electrophysiological effects on canine cardiac Purkinje fibres and feline papillary muscles. In both tissues the drug causes a decrease in resting membrane potential and action potential amplitude as well as spontaneous rate in Purkinje fibres, and an increase in stimulus needed to reach threshold. The minimum effective dose for both tissues was similar at 7.32 x 10-5 M. The drug effects were reversible in both tissues upon removal of the TMB-6. The Ca++ -dependent action potentials of canine cardiac Purkinje fibres were also inhibited by TMB-6 at dose range of 7.32 x 10-5 -24.4 x 10-5 M. Because of its electro physiological effects of threshold, automaticity and \"slow response\" action potentials and its reversibility, TMB-6 could become a useful antiarrhythmic drug.", "PMID": 959320}
{"id": "pubmed23n0032_3370", "title": "The effect of denervation upon the in vitro incorporation of adenosine-8-3H into 3H-adenine nucleotides in the guinea-pig vas deferens.", "content": "After denervation of the guinea-pig vas deferens (1, 2, 4 and 8 days) there is a profound fall in the endogenous levels of adenosine triphosphate (ATP) initially to 54% of control at day 1, with a subsequent rise to 80% by day 8 post-operatively. Theoretically, alterations in the synthetic rate of ATP formation or its utilization could account for the changes in endogenous ATP concentration. These possibilities were evaluated in the present study. Using an in vitro incubation system and adenosine-8-3H as a precursor, experiments demonstrated the feasibility of labeling the synthetic pathway primarily as 3H-AMP, 3H-ADP and 3H-ATP with the latter nucleotide constituting the largest fraction. This incorporation into 3H-nucleotides was linear with time up to 30 min and was dependent on the concentration of adenosine-8-3H used. Studies on the incorporation of adenosine-8-3H at 1, 2, 4 and 8 days after denervation showed no difference in the ability of innervated and denervated vasa deferentia to accumulate total 3H-nucleotides, although there was an alteration in 3H-AMP metabolism in the denervated tissue. Possible explanations for the alteration in 3H-AMP metabolism are discussed. A change in the rate of utilization rather than a change in rate of synthesis, is suggested as an explanation for the alterations in ATP content observed after denervation.", "contents": "The effect of denervation upon the in vitro incorporation of adenosine-8-3H into 3H-adenine nucleotides in the guinea-pig vas deferens. After denervation of the guinea-pig vas deferens (1, 2, 4 and 8 days) there is a profound fall in the endogenous levels of adenosine triphosphate (ATP) initially to 54% of control at day 1, with a subsequent rise to 80% by day 8 post-operatively. Theoretically, alterations in the synthetic rate of ATP formation or its utilization could account for the changes in endogenous ATP concentration. These possibilities were evaluated in the present study. Using an in vitro incubation system and adenosine-8-3H as a precursor, experiments demonstrated the feasibility of labeling the synthetic pathway primarily as 3H-AMP, 3H-ADP and 3H-ATP with the latter nucleotide constituting the largest fraction. This incorporation into 3H-nucleotides was linear with time up to 30 min and was dependent on the concentration of adenosine-8-3H used. Studies on the incorporation of adenosine-8-3H at 1, 2, 4 and 8 days after denervation showed no difference in the ability of innervated and denervated vasa deferentia to accumulate total 3H-nucleotides, although there was an alteration in 3H-AMP metabolism in the denervated tissue. Possible explanations for the alteration in 3H-AMP metabolism are discussed. A change in the rate of utilization rather than a change in rate of synthesis, is suggested as an explanation for the alterations in ATP content observed after denervation.", "PMID": 959321}
{"id": "pubmed23n0032_3371", "title": "The effect of aminophylline on pentagastrin-induced secretion and motor activity of totally isolated canine stomach perfused extracorporeally.", "content": "Totally isolated canine stomachs, perfused extracorporeally with homologous blood of living oxygenerator-dogs, were used for the study of gastric secretion. Aminophylline and pentagastrin were infused into the gastric arterial system separately or combined. Aminophylline, infused alone, did not stimulate gastric secretion. However when aminophylline was administered during the infusion of pentagastrin, it significantly augmented the action of this peptide on gastric acid secretion. Aminophylline did not alter myoelectrical and mechanical response to pentagastrin nor did it affect the myoelectrical activity of the nonstimulated stomach. Gastric arterial perfusion pressure and gastric peripheral resistance were reduced during infusion of aminophylline alone or in combination with pentagastrin.", "contents": "The effect of aminophylline on pentagastrin-induced secretion and motor activity of totally isolated canine stomach perfused extracorporeally. Totally isolated canine stomachs, perfused extracorporeally with homologous blood of living oxygenerator-dogs, were used for the study of gastric secretion. Aminophylline and pentagastrin were infused into the gastric arterial system separately or combined. Aminophylline, infused alone, did not stimulate gastric secretion. However when aminophylline was administered during the infusion of pentagastrin, it significantly augmented the action of this peptide on gastric acid secretion. Aminophylline did not alter myoelectrical and mechanical response to pentagastrin nor did it affect the myoelectrical activity of the nonstimulated stomach. Gastric arterial perfusion pressure and gastric peripheral resistance were reduced during infusion of aminophylline alone or in combination with pentagastrin.", "PMID": 959322}
{"id": "pubmed23n0032_3372", "title": "The reversal of clonidine-induced hypotension by protriptyline and desipramine.", "content": "The present paper deals with further studies on the interaction between clonidine and tricyclic antidepressants. The pronounced central hypotensive action of 1 mug clonidine/kg, administered into the left vertebral artery of chloralose-anaesthetized cats was readily reversed by protriptyline (300 mug/kg), infused via the same route shortly after the development of the maximum hypotensive effect of clonidine. In earlier studies it has been demonstrated that pretreatment with tricyclic antidepressants significantly diminishes the central hypotensive action of clonidine. This interaction has been presumed to occur at the level of central alpha-adrenoreceptors, where clonidine would be the agonist and tricyclic antidepressants the antagonist. The present findings suggest that a competitive antagonism at the central level, which can occur in either sense, may be involved.", "contents": "The reversal of clonidine-induced hypotension by protriptyline and desipramine. The present paper deals with further studies on the interaction between clonidine and tricyclic antidepressants. The pronounced central hypotensive action of 1 mug clonidine/kg, administered into the left vertebral artery of chloralose-anaesthetized cats was readily reversed by protriptyline (300 mug/kg), infused via the same route shortly after the development of the maximum hypotensive effect of clonidine. In earlier studies it has been demonstrated that pretreatment with tricyclic antidepressants significantly diminishes the central hypotensive action of clonidine. This interaction has been presumed to occur at the level of central alpha-adrenoreceptors, where clonidine would be the agonist and tricyclic antidepressants the antagonist. The present findings suggest that a competitive antagonism at the central level, which can occur in either sense, may be involved.", "PMID": 959323}
{"id": "pubmed23n0032_3373", "title": "Different toxic effects of ouabain and 16-epi-gitoxin on Purkinje fibres and ventricular muscle fibres.", "content": "Action potentials of isolated Purkinje fibres and ventricular muscle fibres of canine hearts treated with ouabain and 16-epi-gitoxin were recorded by microelectrodes. Under the influence of both glycosides, Purkinje system fibres became inexcitable earlier than ventricular muscle fibres. Being exposed to 0.125 muM ouabain and 2.5 muM 16-epi-gitoxin, both Purkinje and ventricular muscle fibres became poisoned in the same time as ventricular muscle fibres exposed to 0.1 muM ouabain and 1.5 muM 16-epi-gitoxin. At the lower 16-epi-gitoxin concentration Purkinje fibres had 2.5 times the survival time of that exposed to the lower ouabain concentration. Compared to 16-epi-gitoxin the inexcitability of Purkinje fibres after ouabain remained irreversible. The semisynthetic glycoside 16-epi-gitoxin exerts a weaker effect on the specialized conducting system.", "contents": "Different toxic effects of ouabain and 16-epi-gitoxin on Purkinje fibres and ventricular muscle fibres. Action potentials of isolated Purkinje fibres and ventricular muscle fibres of canine hearts treated with ouabain and 16-epi-gitoxin were recorded by microelectrodes. Under the influence of both glycosides, Purkinje system fibres became inexcitable earlier than ventricular muscle fibres. Being exposed to 0.125 muM ouabain and 2.5 muM 16-epi-gitoxin, both Purkinje and ventricular muscle fibres became poisoned in the same time as ventricular muscle fibres exposed to 0.1 muM ouabain and 1.5 muM 16-epi-gitoxin. At the lower 16-epi-gitoxin concentration Purkinje fibres had 2.5 times the survival time of that exposed to the lower ouabain concentration. Compared to 16-epi-gitoxin the inexcitability of Purkinje fibres after ouabain remained irreversible. The semisynthetic glycoside 16-epi-gitoxin exerts a weaker effect on the specialized conducting system.", "PMID": 959324}
{"id": "pubmed23n0032_3374", "title": "Antinociceptive and stimulant effects of morphine after chemical sympathectomy.", "content": "The modifications produced by 6-hydroxydopamine (6-OHDA) on the analgesic and toxic effects of morphine have been studied in mice and cats. After intracerebral injections of 6-OHDA, mice had a lower threshold for morphine-induced convulsions. Morphine analgesia assayed by the phenylquinone test was apparently antagonized in the 6-OHDA pretreated mice, but the 6-OHDA mice showed more reactivity to the phenylquinone. Intraventricular injection of 6-OHDA in cats produced an acute syndrome with mydriasis, bradycardia, bradypnea, hypothermia and EEG slowing, which subsided after several days, 6-OHDA was successful in blocking the morphine mania, but the animals died within 24 h.", "contents": "Antinociceptive and stimulant effects of morphine after chemical sympathectomy. The modifications produced by 6-hydroxydopamine (6-OHDA) on the analgesic and toxic effects of morphine have been studied in mice and cats. After intracerebral injections of 6-OHDA, mice had a lower threshold for morphine-induced convulsions. Morphine analgesia assayed by the phenylquinone test was apparently antagonized in the 6-OHDA pretreated mice, but the 6-OHDA mice showed more reactivity to the phenylquinone. Intraventricular injection of 6-OHDA in cats produced an acute syndrome with mydriasis, bradycardia, bradypnea, hypothermia and EEG slowing, which subsided after several days, 6-OHDA was successful in blocking the morphine mania, but the animals died within 24 h.", "PMID": 959325}
{"id": "pubmed23n0032_3375", "title": "Management of adult poliomyelitis patients in the Solomon Islands.", "content": "An account is given of a realistic rehabilitation service for the treatment of crawling adult poliomyelitic patients in an area of the developing world. A classification of the crawling patterns encountered is presented. The simple methods by which rehabilitation was carried out for 120 patients are described. Methods consisted of surgery to straighten and align the lower limbs when necessary, the fitting of inexpensive appliances, and the teaching of crutch walking. At long-term review, 80 percent of the patients had responded satisfactorily to treatment. Rehabilitation in an economically and medically poor country can be justified.", "contents": "Management of adult poliomyelitis patients in the Solomon Islands. An account is given of a realistic rehabilitation service for the treatment of crawling adult poliomyelitic patients in an area of the developing world. A classification of the crawling patterns encountered is presented. The simple methods by which rehabilitation was carried out for 120 patients are described. Methods consisted of surgery to straighten and align the lower limbs when necessary, the fitting of inexpensive appliances, and the teaching of crutch walking. At long-term review, 80 percent of the patients had responded satisfactorily to treatment. Rehabilitation in an economically and medically poor country can be justified.", "PMID": 959326}
{"id": "pubmed23n0032_3376", "title": "Electromagnetic field interference and cardiac pacemakers.", "content": "The physical and physiological effects of electromagnetic field interference on 440 patients with cardiac pacemakers were determined by reviewing the literature from 1949 to 1973. The sources, mechanisms, and findings of physiological dysfunction and ventricular fibrillation in patients with pacemakers are presented. Shortwave and microwave diathermy and electrical stimulators have been found to have a definite adverse influence on some cardiac pacemakers. The effect of interference may be an increase or decrease in pacemaker rate or rhythm, ventricular fibrillation, a total loss of pacing, or cessation of impulses. Because all pacemaker units are not resistant to interference, no ungrounded electrical equipment and no equipment such as microwave diathermy, short wave diathermy, and electrical stimulators should be placed on, or near, a patient with a cardiac pacemaker.", "contents": "Electromagnetic field interference and cardiac pacemakers. The physical and physiological effects of electromagnetic field interference on 440 patients with cardiac pacemakers were determined by reviewing the literature from 1949 to 1973. The sources, mechanisms, and findings of physiological dysfunction and ventricular fibrillation in patients with pacemakers are presented. Shortwave and microwave diathermy and electrical stimulators have been found to have a definite adverse influence on some cardiac pacemakers. The effect of interference may be an increase or decrease in pacemaker rate or rhythm, ventricular fibrillation, a total loss of pacing, or cessation of impulses. Because all pacemaker units are not resistant to interference, no ungrounded electrical equipment and no equipment such as microwave diathermy, short wave diathermy, and electrical stimulators should be placed on, or near, a patient with a cardiac pacemaker.", "PMID": 959327}
{"id": "pubmed23n0032_3377", "title": "Metabolic energy cost of unrestrained walking.", "content": "Physiologic factors of metabolic energy cost as well as selected mechanical characteristics of gait are described in a group of 40 presumably normal men and women between the ages of 20 and 60 years. Special emphasis was placed on unrestrained (free cadence) walking to provide a reliable baseline for comparison to persons with physical gait impairments. Velocity of walking was the most important factor in determining oxygen uptake and was independent of age or sex. An empirical equation was established relating oxygen uptake to the speed of walking. The average velocity for men in unrestrained walking trials was 89 meters per minute; for women 74 meters per minute. These differences were related to the greater stride length in men. The average cadence selected by both sexes was 116 steps per minute. None of these factors was age dependent. Of the physiologic measures only systolic blood pressure was age dependent predictably rising with age. The mean heart rate was 103 beats per minute and did not vary significantly between men and women. The mean respiratory rate was 19 per minute; the mean respiratory quotient, 0.85, neither being age or sex dependent. Oxygen uptake values averaged 12.95 ml/kg-min for the population studied and again were neither age nor sex dependent.", "contents": "Metabolic energy cost of unrestrained walking. Physiologic factors of metabolic energy cost as well as selected mechanical characteristics of gait are described in a group of 40 presumably normal men and women between the ages of 20 and 60 years. Special emphasis was placed on unrestrained (free cadence) walking to provide a reliable baseline for comparison to persons with physical gait impairments. Velocity of walking was the most important factor in determining oxygen uptake and was independent of age or sex. An empirical equation was established relating oxygen uptake to the speed of walking. The average velocity for men in unrestrained walking trials was 89 meters per minute; for women 74 meters per minute. These differences were related to the greater stride length in men. The average cadence selected by both sexes was 116 steps per minute. None of these factors was age dependent. Of the physiologic measures only systolic blood pressure was age dependent predictably rising with age. The mean heart rate was 103 beats per minute and did not vary significantly between men and women. The mean respiratory rate was 19 per minute; the mean respiratory quotient, 0.85, neither being age or sex dependent. Oxygen uptake values averaged 12.95 ml/kg-min for the population studied and again were neither age nor sex dependent.", "PMID": 959328}
{"id": "pubmed23n0032_3378", "title": "Cardiac rehabilitation in Germany.", "content": "The concept of cardiac reconditioning centers for the prevention and rehabilitation of coronary patients has been tremendously successful in Germany over the past 20 years. At least 40 such centers are located throughout the country. Physicians, nurses, and physical therapists work closely together in the various facets of the rehabilitation process. The financial backing for these facilities is primarily through governmental and regional insurance companies, whose officials are apparently convinced that in the long run supporting preventive measures is financially sound. Objective data supporting their convictions come from studies such as that of Brusis, who showed that such as that of 1,500 employees was diminished by nearly 70 percent during a two-year period after cardiac reconditioning, as compared to a similar time period before the rehabilitation experience. Subjective benefits, which are extremely difficult to quantitate in meaningful terms, were nonetheless expressed by nearly all the patients with whom I conversed. Perhaps they have experienced the same feelings that Mark Twain did when he observed that \"all frets and worries and chafings sank to sleep in the presence of the benignant serenity of the Alps; the Great Spirit of the Mountains breathed his own peace upon their hurt minds and sore hearts and healed them.\"", "contents": "Cardiac rehabilitation in Germany. The concept of cardiac reconditioning centers for the prevention and rehabilitation of coronary patients has been tremendously successful in Germany over the past 20 years. At least 40 such centers are located throughout the country. Physicians, nurses, and physical therapists work closely together in the various facets of the rehabilitation process. The financial backing for these facilities is primarily through governmental and regional insurance companies, whose officials are apparently convinced that in the long run supporting preventive measures is financially sound. Objective data supporting their convictions come from studies such as that of Brusis, who showed that such as that of 1,500 employees was diminished by nearly 70 percent during a two-year period after cardiac reconditioning, as compared to a similar time period before the rehabilitation experience. Subjective benefits, which are extremely difficult to quantitate in meaningful terms, were nonetheless expressed by nearly all the patients with whom I conversed. Perhaps they have experienced the same feelings that Mark Twain did when he observed that \"all frets and worries and chafings sank to sleep in the presence of the benignant serenity of the Alps; the Great Spirit of the Mountains breathed his own peace upon their hurt minds and sore hearts and healed them.\"", "PMID": 959329}
{"id": "pubmed23n0032_3379", "title": "Bronchial drainage with aerosol medications in cystic fibrosis.", "content": "Examined in this study are the immediate effects of bronchial drainage preceded by the inhalation of aerosol solutions of a mucolytic agent, a bronchodilator, and their combination upon four measures of pulmonary function in 20 patients with cystic fibrosis. On three separate occasions, pulmonary function was tested preceding and following treatments consisting of one of the above three aerosol solutions followed by bronchial drainage. The order of solutions used was random and each subject received one treatment with each solution. The greatest overall improvement in function was seen with the bronchodilator and bronchial drainage. The combination of the bronchodilator/mucolytic agents and bronchial drainage was the next most effective technique. The mucolytic agent, when used alone with bronchial drainage, decreased function. Comparison of the pulmonary function scores indicated a significant difference among the three treatments.", "contents": "Bronchial drainage with aerosol medications in cystic fibrosis. Examined in this study are the immediate effects of bronchial drainage preceded by the inhalation of aerosol solutions of a mucolytic agent, a bronchodilator, and their combination upon four measures of pulmonary function in 20 patients with cystic fibrosis. On three separate occasions, pulmonary function was tested preceding and following treatments consisting of one of the above three aerosol solutions followed by bronchial drainage. The order of solutions used was random and each subject received one treatment with each solution. The greatest overall improvement in function was seen with the bronchodilator and bronchial drainage. The combination of the bronchodilator/mucolytic agents and bronchial drainage was the next most effective technique. The mucolytic agent, when used alone with bronchial drainage, decreased function. Comparison of the pulmonary function scores indicated a significant difference among the three treatments.", "PMID": 959332}
{"id": "pubmed23n0032_3380", "title": "Proposed role of the alpha subunit of the 7 S nerve growth factor protein: implications for the regulation of NGF activity.", "content": "Four electrophoretically distinct alpha subunits can be isolated from mouse 7 S nerve growth factor protein (7 S NGF). It is proposed that each alpha subunit confers on its 7 S NGF species a different dissociation constant, and that the heterogeneity in dissociation constants of the different 7 S NGF species provide a mechanism for regulating the structure of betaNGF. A hypothesis that the different betaNGF structures have altered receptor affinities and/or physiological properties which can functionally differentiate NGF activity is also presented.", "contents": "Proposed role of the alpha subunit of the 7 S nerve growth factor protein: implications for the regulation of NGF activity. Four electrophoretically distinct alpha subunits can be isolated from mouse 7 S nerve growth factor protein (7 S NGF). It is proposed that each alpha subunit confers on its 7 S NGF species a different dissociation constant, and that the heterogeneity in dissociation constants of the different 7 S NGF species provide a mechanism for regulating the structure of betaNGF. A hypothesis that the different betaNGF structures have altered receptor affinities and/or physiological properties which can functionally differentiate NGF activity is also presented.", "PMID": 959333}
{"id": "pubmed23n0032_3381", "title": "Effect of norepinephrine on the phospholipid composition of blood plasma and red blood cells in anesthetized dogs.", "content": "The phospholipid composition of blood plasma and erythrocytes from artery, hepatic venous, and sagittal sinus blood was investigated in dogs under pentobarbital anesthesia both before and after continuous infusion of norepinephrine. Norepinephrine infusion caused a very significant increase of phosphatidylglycerol (GPG) in the erythrocytes and plasma from arterial blood. Other phospholipids were not affected by the infusion. Significant release of GPG across the brain and splanchnic area was noted both in plasma and erythrocytes prior to, but not following, norepinephrine infusion. The importance of the differences between the respective phospholipid compositions of erythrocytes and plasma is emphasized. The biologic significance of the changes of the phospholipids, particularly of GPG during stress, is discussed.", "contents": "Effect of norepinephrine on the phospholipid composition of blood plasma and red blood cells in anesthetized dogs. The phospholipid composition of blood plasma and erythrocytes from artery, hepatic venous, and sagittal sinus blood was investigated in dogs under pentobarbital anesthesia both before and after continuous infusion of norepinephrine. Norepinephrine infusion caused a very significant increase of phosphatidylglycerol (GPG) in the erythrocytes and plasma from arterial blood. Other phospholipids were not affected by the infusion. Significant release of GPG across the brain and splanchnic area was noted both in plasma and erythrocytes prior to, but not following, norepinephrine infusion. The importance of the differences between the respective phospholipid compositions of erythrocytes and plasma is emphasized. The biologic significance of the changes of the phospholipids, particularly of GPG during stress, is discussed.", "PMID": 959334}
{"id": "pubmed23n0032_3382", "title": "Proton magnetic resonance (PMR) studies of water in dimethyl sulfoxide perfused rat myocardium.", "content": "Time-dependent studies of the PMR linewidths and spin-lattice relaxation times (T1) of rat myocardium perfused with chemically-defined tissue culture medium containing 15% dimethyl sulfoxide (DMSO) (v/v) were performed. In comparison with \"normal\" myocardium samples and samples from hearts perfused without DSMO, linewidths initially increased following perfusion of the hearts with 15% DMSO solution. However, the increase was observable only during the first five minutes following perfusion, subsequently narrowing considerably. A minimum T1 was obtained 30 minutes post-treatment. The experiments indicate translocation of intracellular water into bulk-like presumably extracellular water, thus suggesting a cellular dehydration effect of DMSO.", "contents": "Proton magnetic resonance (PMR) studies of water in dimethyl sulfoxide perfused rat myocardium. Time-dependent studies of the PMR linewidths and spin-lattice relaxation times (T1) of rat myocardium perfused with chemically-defined tissue culture medium containing 15% dimethyl sulfoxide (DMSO) (v/v) were performed. In comparison with \"normal\" myocardium samples and samples from hearts perfused without DSMO, linewidths initially increased following perfusion of the hearts with 15% DMSO solution. However, the increase was observable only during the first five minutes following perfusion, subsequently narrowing considerably. A minimum T1 was obtained 30 minutes post-treatment. The experiments indicate translocation of intracellular water into bulk-like presumably extracellular water, thus suggesting a cellular dehydration effect of DMSO.", "PMID": 959337}
{"id": "pubmed23n0032_3383", "title": "Observations on the myth of \"informed consent\".", "content": "A series of 100 patients in a general plastic surgery population were interviewed by standard format to determine their retention rate of preoperative information given to them. An overall recall, or retention rate, of 35 percent was found.", "contents": "Observations on the myth of \"informed consent\". A series of 100 patients in a general plastic surgery population were interviewed by standard format to determine their retention rate of preoperative information given to them. An overall recall, or retention rate, of 35 percent was found.", "PMID": 959403}
{"id": "pubmed23n0032_3384", "title": "Surgical treatment of impotence in the male. An 18-year experience with 250 penile implants.", "content": "We have treated more than 250 cases of impotence by inserting a penile rod. The implant has been modified intermittently, and it currently consists of a two-piece rod with a Y-shaped proximal portion to provide greater stability. Most of the patients we treated have been satisfied with the results of the surgery, and would have it repeated. Therefore, we feel that this prosthesis is an excellent one for treating male impotence.", "contents": "Surgical treatment of impotence in the male. An 18-year experience with 250 penile implants. We have treated more than 250 cases of impotence by inserting a penile rod. The implant has been modified intermittently, and it currently consists of a two-piece rod with a Y-shaped proximal portion to provide greater stability. Most of the patients we treated have been satisfied with the results of the surgery, and would have it repeated. Therefore, we feel that this prosthesis is an excellent one for treating male impotence.", "PMID": 959404}
{"id": "pubmed23n0032_3385", "title": "The value of routine breast biopsy at the time of augmentation mammaplasty.", "content": "A series of patients who had biopsies of breast nodules during augmentation mammaplasty is reviewed. We conclude that such procedures performed for discrete lesions yield valuable information. In this series, the biopsies did not result in an increased rate of complications.", "contents": "The value of routine breast biopsy at the time of augmentation mammaplasty. A series of patients who had biopsies of breast nodules during augmentation mammaplasty is reviewed. We conclude that such procedures performed for discrete lesions yield valuable information. In this series, the biopsies did not result in an increased rate of complications.", "PMID": 959405}
{"id": "pubmed23n0032_3386", "title": "The Byars hypospadias repair. A review of 253 consecutive patients.", "content": "Of 253 consecutive hypospadias repairs by various surgeons, using the Byars technique, 57 (22 percent) had postoperative fistulae; 41 required one operation, 9 required two operations, and 7 required more than two operations for closure of these fistulae. Other complications were infrequent. When performed either by attending or resident physicians, the Byars hypospadias repair produced complete correction of the chordee, a urethra of satisfactory length and caliber, normal penile appearance, and a fistula rate comparable to that reported after other procedures.", "contents": "The Byars hypospadias repair. A review of 253 consecutive patients. Of 253 consecutive hypospadias repairs by various surgeons, using the Byars technique, 57 (22 percent) had postoperative fistulae; 41 required one operation, 9 required two operations, and 7 required more than two operations for closure of these fistulae. Other complications were infrequent. When performed either by attending or resident physicians, the Byars hypospadias repair produced complete correction of the chordee, a urethra of satisfactory length and caliber, normal penile appearance, and a fistula rate comparable to that reported after other procedures.", "PMID": 959407}
{"id": "pubmed23n0032_3387", "title": "Long-term results of the island flap palatal pushback.", "content": "We analyzed all 104 island flap pushback palatal repairs done through our Facial Rehabilitation Clinic in the period from 1965 to 1971. The results were compared to those in a group of 109 standard pushback repairs. The island flap group had a higher incidence of operative complications, of velopharyngeal insufficiency, and of secondary procedures to correct the latter. (The differences between the two groups were not statistically significant, however.) In this group of patients the island flap repair offered no particular advantage over the V-Y or the Dorrance pushback; in fact, it may have been deleterious. A hypothetical explanation for these results is offered, based on possible continuing osteogenesis by the transplanted mucoperiosteum, to produce an inflexible and poorly functioning velum.", "contents": "Long-term results of the island flap palatal pushback. We analyzed all 104 island flap pushback palatal repairs done through our Facial Rehabilitation Clinic in the period from 1965 to 1971. The results were compared to those in a group of 109 standard pushback repairs. The island flap group had a higher incidence of operative complications, of velopharyngeal insufficiency, and of secondary procedures to correct the latter. (The differences between the two groups were not statistically significant, however.) In this group of patients the island flap repair offered no particular advantage over the V-Y or the Dorrance pushback; in fact, it may have been deleterious. A hypothetical explanation for these results is offered, based on possible continuing osteogenesis by the transplanted mucoperiosteum, to produce an inflexible and poorly functioning velum.", "PMID": 959408}
{"id": "pubmed23n0032_3388", "title": "A new method of treatment for mallet finger. A preliminary report.", "content": "We describe a new technique to treat the mallet finger deformity. The method is both simple and efficient, and it does not require follow-up for splint or dressing adjustment. All of our 15 cases regained function without complications.", "contents": "A new method of treatment for mallet finger. A preliminary report. We describe a new technique to treat the mallet finger deformity. The method is both simple and efficient, and it does not require follow-up for splint or dressing adjustment. All of our 15 cases regained function without complications.", "PMID": 959409}
{"id": "pubmed23n0032_3389", "title": "Dacryoscintigraph evaluation of the repaired lacrimal drainage system.", "content": "We have used dacryoscintigraphy to study the efficiency of the lacrimal system following the repair of a divided lower canaliculus in 4 children. This test has been reliable in evaluating lacrimal function and suggests that a canaliculus can be repaired and splinted satisfactorily. Our experience with dacryoscintigraphy confirms that it is a safe comfortable, and reliable method for studying the patency of the lacrimal drainage system.", "contents": "Dacryoscintigraph evaluation of the repaired lacrimal drainage system. We have used dacryoscintigraphy to study the efficiency of the lacrimal system following the repair of a divided lower canaliculus in 4 children. This test has been reliable in evaluating lacrimal function and suggests that a canaliculus can be repaired and splinted satisfactorily. Our experience with dacryoscintigraphy confirms that it is a safe comfortable, and reliable method for studying the patency of the lacrimal drainage system.", "PMID": 959410}
{"id": "pubmed23n0032_3390", "title": "Use of a buried nasolabial flap to fill a depression over the lower jaw. Case report.", "content": "A viable local flap is not plagued by the problems of resorption and dissolution. If it is to be denuded and buried, however, great care must be taken to select a donor area that is as hairless as possible. Otherwise, there may be cyst formation, drainage, and infection.", "contents": "Use of a buried nasolabial flap to fill a depression over the lower jaw. Case report. A viable local flap is not plagued by the problems of resorption and dissolution. If it is to be denuded and buried, however, great care must be taken to select a donor area that is as hairless as possible. Otherwise, there may be cyst formation, drainage, and infection.", "PMID": 959412}
{"id": "pubmed23n0032_3391", "title": "Use of nasopharyngeal tubes as aids to lateral port construction and maintenance of the airway in pharyngeal flap surgery.", "content": "We suggest the bilateral placement of nasopharyngeal tubes of appropriate size as a simple aid in constructing lateral ports of the correct size when doing a superiorly-based, lined, pharyngeal flap for velopharyngeal incompetence. If one leaves the tubes in for 24 to 48 hours postoperatively, they are of great help in maintaining a clear airway during that period.", "contents": "Use of nasopharyngeal tubes as aids to lateral port construction and maintenance of the airway in pharyngeal flap surgery. We suggest the bilateral placement of nasopharyngeal tubes of appropriate size as a simple aid in constructing lateral ports of the correct size when doing a superiorly-based, lined, pharyngeal flap for velopharyngeal incompetence. If one leaves the tubes in for 24 to 48 hours postoperatively, they are of great help in maintaining a clear airway during that period.", "PMID": 959414}
{"id": "pubmed23n0032_3392", "title": "Free muscle transplantation by microsurgical technique to treat severe Volkmann's contracture.", "content": "In a free muscle transfer, great care must be taken in selecting the muscle to be grafted. We have found the pectoralis major to be a good donor muscle. Although most muscles are not nourished by a single artery and a few veins, the vessels which enter the pectoralis major muscle with the nerves are the main nutrient vessels, and these vessels alone can adequately nourish this muscle. Neurorrhaphy should be performed at a site as close as possible to the muscle. Good indications for free muscle transfer are cases in which a flexor muscle has become necrotic, while the extensor muscle is viable--for example, if the flexor muscle has sustained traumatic crushing injury or if there is a Volkmann's contracture.", "contents": "Free muscle transplantation by microsurgical technique to treat severe Volkmann's contracture. In a free muscle transfer, great care must be taken in selecting the muscle to be grafted. We have found the pectoralis major to be a good donor muscle. Although most muscles are not nourished by a single artery and a few veins, the vessels which enter the pectoralis major muscle with the nerves are the main nutrient vessels, and these vessels alone can adequately nourish this muscle. Neurorrhaphy should be performed at a site as close as possible to the muscle. Good indications for free muscle transfer are cases in which a flexor muscle has become necrotic, while the extensor muscle is viable--for example, if the flexor muscle has sustained traumatic crushing injury or if there is a Volkmann's contracture.", "PMID": 959415}
{"id": "pubmed23n0032_3393", "title": "Augmentation mammaplasty by the transaxillary approach.", "content": "Augmentation mammaplasty by the transaxillary approach seems to be a safe and anatomically sound procedure which produces consistent results with a low complication rate. Our experiences with this procedure and our modifications in the technique are described. Two instruments which were designed to facilitate the procedure are reported. An anatomical justification for the transaxillary method is made.", "contents": "Augmentation mammaplasty by the transaxillary approach. Augmentation mammaplasty by the transaxillary approach seems to be a safe and anatomically sound procedure which produces consistent results with a low complication rate. Our experiences with this procedure and our modifications in the technique are described. Two instruments which were designed to facilitate the procedure are reported. An anatomical justification for the transaxillary method is made.", "PMID": 959416}
{"id": "pubmed23n0032_3394", "title": "\"Lethal midline granuloma\".", "content": "We have recently had two cases of midfacial granulomas in which laboratory examinations and multiple biopsies did not reveal a more specific diagnosis. Both responded to treatment. We feel that in such cases therapy should be given and based on the extent of local destruction and the history of the disease in the patient.", "contents": "\"Lethal midline granuloma\". We have recently had two cases of midfacial granulomas in which laboratory examinations and multiple biopsies did not reveal a more specific diagnosis. Both responded to treatment. We feel that in such cases therapy should be given and based on the extent of local destruction and the history of the disease in the patient.", "PMID": 959417}
{"id": "pubmed23n0032_3395", "title": "Evaluation of digital escharotomy in burned hands.", "content": "A controlled study of digital escharotomy showed a statistically significant increase in the number of salvaged phalanges. This procedure has minimal risk and should be considered in patients with vascular compromise of the upper extremity requiring escharotomy, who also have circumferentially burned fingers.", "contents": "Evaluation of digital escharotomy in burned hands. A controlled study of digital escharotomy showed a statistically significant increase in the number of salvaged phalanges. This procedure has minimal risk and should be considered in patients with vascular compromise of the upper extremity requiring escharotomy, who also have circumferentially burned fingers.", "PMID": 959418}
{"id": "pubmed23n0032_3396", "title": "Excision of massive hemangioneurofibroma of the face.", "content": "Nevus flammeus (cutaneous hemangioma, port-wine stain) may develop proliferative hamartomatous changes and go on to form monstrous deforming tumors. Excision is feasible, and it may be best to do it early. The mentally retarded, institutionalized patient can also benefit from the reconstruction. In 3 cases it was possible to preserve function of the muscles of facial expression, because of the superficial location of the tumor. Techniques to decrease blood loss are of variable value during the operative procedure.", "contents": "Excision of massive hemangioneurofibroma of the face. Nevus flammeus (cutaneous hemangioma, port-wine stain) may develop proliferative hamartomatous changes and go on to form monstrous deforming tumors. Excision is feasible, and it may be best to do it early. The mentally retarded, institutionalized patient can also benefit from the reconstruction. In 3 cases it was possible to preserve function of the muscles of facial expression, because of the superficial location of the tumor. Techniques to decrease blood loss are of variable value during the operative procedure.", "PMID": 959419}
{"id": "pubmed23n0032_3397", "title": "The mucoperiosteal palate flap for closure of oral cavity defects.", "content": "We have used a modification of a mucoperiosteal flap described by Lane for cleft palates in 3 patients with oral cavity defects from tumor ablations. The flap provides excellent lining for repairs when alternative flaps are unavailable. The vascularity of the flap and its texture allow a watertight closure which does not hinder the patient's ability to talk or eat.", "contents": "The mucoperiosteal palate flap for closure of oral cavity defects. We have used a modification of a mucoperiosteal flap described by Lane for cleft palates in 3 patients with oral cavity defects from tumor ablations. The flap provides excellent lining for repairs when alternative flaps are unavailable. The vascularity of the flap and its texture allow a watertight closure which does not hinder the patient's ability to talk or eat.", "PMID": 959420}
{"id": "pubmed23n0032_3398", "title": "Microvascular anastomosis: an experimental model utilizing the rabbit ear.", "content": "Using the rabbit ear as an experimental model, a composite flap based on an anastomosed central artery and vein can be created and its viability determined. This inexpensive model can be used to train plastic surgical residents in microsurgery and in further investigations of the microcirculation of flaps and their survival.", "contents": "Microvascular anastomosis: an experimental model utilizing the rabbit ear. Using the rabbit ear as an experimental model, a composite flap based on an anastomosed central artery and vein can be created and its viability determined. This inexpensive model can be used to train plastic surgical residents in microsurgery and in further investigations of the microcirculation of flaps and their survival.", "PMID": 959421}
{"id": "pubmed23n0032_3399", "title": "Species differences in the trypsin separation of epidermis and dermis.", "content": "Trypsin incubation was performed on split-skin grafts from rats, pigs, and humans. The separation of dermis and epidermis was best achieved when incubating in 0.5% crude trypsin in Hank's balanced salt solution. Separation occurred at 37 degrees C in 1 1/2 hours for all species. At 4 degrees C, the separation took 2 1/2 hours for pigskin, 3 1/2 to 4 1/2 hours for human skin, and 5 to 6 hours for rat skin. Histological examination showed a cleaner separation of both rat skin and pigskin was obtained at 4 degrees C than at 37 degrees C. Large areas of skin (1600 mm2) could be separated easily as long as adequate amounts of solution were used.", "contents": "Species differences in the trypsin separation of epidermis and dermis. Trypsin incubation was performed on split-skin grafts from rats, pigs, and humans. The separation of dermis and epidermis was best achieved when incubating in 0.5% crude trypsin in Hank's balanced salt solution. Separation occurred at 37 degrees C in 1 1/2 hours for all species. At 4 degrees C, the separation took 2 1/2 hours for pigskin, 3 1/2 to 4 1/2 hours for human skin, and 5 to 6 hours for rat skin. Histological examination showed a cleaner separation of both rat skin and pigskin was obtained at 4 degrees C than at 37 degrees C. Large areas of skin (1600 mm2) could be separated easily as long as adequate amounts of solution were used.", "PMID": 959422}
{"id": "pubmed23n0032_3400", "title": "Prevention of thrombosis in arterial and venous microanastomoses by using topical agents.", "content": "A replantation model, using the rat's foot severed at the ankle and replanted on an extended vascular pedicle, was used to study the effect of topical vasodilators in preventing microvascular thrombosis. Magnesium sulfate, Xylocaine, and papaverine gave arterial patency rates of 89% or more, compared to 65% for saline alone. There was no appreciable effect on the venous patency rates. The possible mechanisms of action for the various drugs are discussed.", "contents": "Prevention of thrombosis in arterial and venous microanastomoses by using topical agents. A replantation model, using the rat's foot severed at the ankle and replanted on an extended vascular pedicle, was used to study the effect of topical vasodilators in preventing microvascular thrombosis. Magnesium sulfate, Xylocaine, and papaverine gave arterial patency rates of 89% or more, compared to 65% for saline alone. There was no appreciable effect on the venous patency rates. The possible mechanisms of action for the various drugs are discussed.", "PMID": 959424}
{"id": "pubmed23n0032_3401", "title": "A method for studying endothelial regeneration in small vessels.", "content": "A simple, accurate method for demonstrating very small areas of endothelium is described. Some possible fields for further investigation are suggested. An outline of the possible normal mechanism of endothelial regeneration is described.", "contents": "A method for studying endothelial regeneration in small vessels. A simple, accurate method for demonstrating very small areas of endothelium is described. Some possible fields for further investigation are suggested. An outline of the possible normal mechanism of endothelial regeneration is described.", "PMID": 959425}
{"id": "pubmed23n0032_3402", "title": "Benign atypical fibroxanthoma or a malignant tumor? A warning. Case reports.", "content": "Atypical fibroxanthoma of the skin has been recognized recently as a pseudo-malignant tumor, probably reactive in nature. By all the classic morphological criteria, the lesion appears to be malignant--but in behavior it is benign. In older patients the head and neck are primarily involved, while in younger patients these tumors appear on the covered areas of the trunk and limbs. Complete local excision is considered to be adequate treatment.", "contents": "Benign atypical fibroxanthoma or a malignant tumor? A warning. Case reports. Atypical fibroxanthoma of the skin has been recognized recently as a pseudo-malignant tumor, probably reactive in nature. By all the classic morphological criteria, the lesion appears to be malignant--but in behavior it is benign. In older patients the head and neck are primarily involved, while in younger patients these tumors appear on the covered areas of the trunk and limbs. Complete local excision is considered to be adequate treatment.", "PMID": 959427}
{"id": "pubmed23n0032_3403", "title": "[Pathological we-formation and dominance shifts in symbiotic psychoses].", "content": "The case history of symbiotic psychosis observed in a mother and her daughter is used to demonstrate the causes of origin of such forms of psychosis, the emphasis being on the description of a rare case of change of dominance. The paper also discusses the reactions shown by person coming in contact with such sychotics and, more particularly, by the partners of individuals with delusional symptomatologies.", "contents": "[Pathological we-formation and dominance shifts in symbiotic psychoses]. The case history of symbiotic psychosis observed in a mother and her daughter is used to demonstrate the causes of origin of such forms of psychosis, the emphasis being on the description of a rare case of change of dominance. The paper also discusses the reactions shown by person coming in contact with such sychotics and, more particularly, by the partners of individuals with delusional symptomatologies.", "PMID": 959429}
{"id": "pubmed23n0032_3404", "title": "[Experience with special classes for mildly brain-damaged children with behavior disorders].", "content": "Within the framework of a large-scale medical and pedagogical experiment conducted at Rostock, two special education classes for second graders were set up at the start of the 1965/66 school year in an attempt to prevent children with cerebral lesions from failing to pass courses already in the lower grades at elementary schools. As a result of this experiment, it was decided to start a school for special education children, and this school now has two parallel classes each for second, third, and fourth graders as well as one additional class in which pupils are prepared for the fifth normal education school grade. Psychological tests made as part of a longitudinal study in which 48 children who had been to this school from 1965, 1966, and 1967 were included and which spread over years including the 1971/72 school years, showed an improvement in motor development with slightly retarded intellectual development and unchanged lack of concentration power. Average attainments in the various subjects improved while pupils were attending special education classes and did not, after reintegration of such children into normal schools (fifth grade), drop below the general level of proficiency.", "contents": "[Experience with special classes for mildly brain-damaged children with behavior disorders]. Within the framework of a large-scale medical and pedagogical experiment conducted at Rostock, two special education classes for second graders were set up at the start of the 1965/66 school year in an attempt to prevent children with cerebral lesions from failing to pass courses already in the lower grades at elementary schools. As a result of this experiment, it was decided to start a school for special education children, and this school now has two parallel classes each for second, third, and fourth graders as well as one additional class in which pupils are prepared for the fifth normal education school grade. Psychological tests made as part of a longitudinal study in which 48 children who had been to this school from 1965, 1966, and 1967 were included and which spread over years including the 1971/72 school years, showed an improvement in motor development with slightly retarded intellectual development and unchanged lack of concentration power. Average attainments in the various subjects improved while pupils were attending special education classes and did not, after reintegration of such children into normal schools (fifth grade), drop below the general level of proficiency.", "PMID": 959430}
{"id": "pubmed23n0032_3405", "title": "[Symptomatology and pathogenesis of Fisher's syndrome].", "content": "The symptomatology of Fisher's syndrome is described with reference to observations made on six own cases as well as to fifty cases described in the literature, and it is shown that, first, ataxia is not an obligatory symptom and that, second, symptoms are an expression, not of a lesion of the brain stem, but of an affection of the peripheral neuron.", "contents": "[Symptomatology and pathogenesis of Fisher's syndrome]. The symptomatology of Fisher's syndrome is described with reference to observations made on six own cases as well as to fifty cases described in the literature, and it is shown that, first, ataxia is not an obligatory symptom and that, second, symptoms are an expression, not of a lesion of the brain stem, but of an affection of the peripheral neuron.", "PMID": 959431}
{"id": "pubmed23n0032_3406", "title": "[The problem of latent neuropathy in essential hypertension].", "content": "A neurographic examination of 54 patients with essential hypertonia did not reveal any significant differences, from a control group, in the maximum rate of motor conduction of the ulnar nerve. An assocation of subclinical neuropathy with essential hypertonia cannot, therefore, be confirmed, thus denying the statement made by Viskoper and his coworkers and saying that the motor nerve conduction speed was an objective index for determining the severity of diseases characterized by hypertension.", "contents": "[The problem of latent neuropathy in essential hypertension]. A neurographic examination of 54 patients with essential hypertonia did not reveal any significant differences, from a control group, in the maximum rate of motor conduction of the ulnar nerve. An assocation of subclinical neuropathy with essential hypertonia cannot, therefore, be confirmed, thus denying the statement made by Viskoper and his coworkers and saying that the motor nerve conduction speed was an objective index for determining the severity of diseases characterized by hypertension.", "PMID": 959432}
{"id": "pubmed23n0032_3407", "title": "Some thoughts on the ego ideal. A contribution to the study of the \"illness of ideality\".", "content": "Man searches continuously for the time when he was his own ideal--a time that is coincident with primary fusion with the mother. The fantasy of this fusion underlies the incest wish. Because of his helplessness, the child must postpone the realization of his incest wish and project it forward into the future. However, if the subject chooses the pathway of the oedipus complex and identification with the father to realize his incestuous fantasy, circumstance may lead him to circumvent the oedipal shpere and, through the mechanisms of regression, return to the mother of primary fusion. As in Aesop's adage, the ego ideal is at the source of the best and the worst of things.", "contents": "Some thoughts on the ego ideal. A contribution to the study of the \"illness of ideality\". Man searches continuously for the time when he was his own ideal--a time that is coincident with primary fusion with the mother. The fantasy of this fusion underlies the incest wish. Because of his helplessness, the child must postpone the realization of his incest wish and project it forward into the future. However, if the subject chooses the pathway of the oedipus complex and identification with the father to realize his incestuous fantasy, circumstance may lead him to circumvent the oedipal shpere and, through the mechanisms of regression, return to the mother of primary fusion. As in Aesop's adage, the ego ideal is at the source of the best and the worst of things.", "PMID": 959433}
{"id": "pubmed23n0032_3408", "title": "A note on internalization as process.", "content": "To the view of internalization as fantasy, there is counterposed the view of internalization as process. The philosophical roots and basic argument of the \"fantasy\" view are evaluated, particularly in reference to the position of metaphorical terms in psychoanalytic theory. Especially confusing is the literal interpretation of metaphors of spatialization distinguishing the inner dimensions of psychic experience from external referents. The persistent theoretical need is to translate metaphorical usage into meaningful and consistent theoretical terms. The organization of introjects cannot be reduced to memory elements, object representations, or fantasy products. Internalization as process refers to a series of real psychological events whose specific delineation and description has not been accomplished.", "contents": "A note on internalization as process. To the view of internalization as fantasy, there is counterposed the view of internalization as process. The philosophical roots and basic argument of the \"fantasy\" view are evaluated, particularly in reference to the position of metaphorical terms in psychoanalytic theory. Especially confusing is the literal interpretation of metaphors of spatialization distinguishing the inner dimensions of psychic experience from external referents. The persistent theoretical need is to translate metaphorical usage into meaningful and consistent theoretical terms. The organization of introjects cannot be reduced to memory elements, object representations, or fantasy products. Internalization as process refers to a series of real psychological events whose specific delineation and description has not been accomplished.", "PMID": 959434}
{"id": "pubmed23n0032_3409", "title": "On parallel processes in therapy and teaching.", "content": "Using the format of a continuous case conference, the authors demonstrate that the therapist-presenter develops unconscious identifications with the patient which are especially intense when difficulties (resistances) arise which he cannot resolve. The presenter then enacts these identifications in the seminar giving rise to responses from the seminar members which repeat the difficulties of the therapy. These parallel processes can be made explicit by eliciting the empathic responses of the seminar participants. By interpreting these parallelisms the authors have evolved a teaching methodology which, like therapy itself, provides an emotionally based learning experience for the participants.", "contents": "On parallel processes in therapy and teaching. Using the format of a continuous case conference, the authors demonstrate that the therapist-presenter develops unconscious identifications with the patient which are especially intense when difficulties (resistances) arise which he cannot resolve. The presenter then enacts these identifications in the seminar giving rise to responses from the seminar members which repeat the difficulties of the therapy. These parallel processes can be made explicit by eliciting the empathic responses of the seminar participants. By interpreting these parallelisms the authors have evolved a teaching methodology which, like therapy itself, provides an emotionally based learning experience for the participants.", "PMID": 959435}
{"id": "pubmed23n0032_3410", "title": "Some problems in faculty selection of patients for supervised psychoanalysis.", "content": "The selection of patients for supervised analysis by candidates involves special tasks in evaluation and prediction. A number of problems emerge when the entire faculty of an institute participates in evaluating such patients. These include the different ways in which the initial consultant and the Admitting Psychoanalyst view their roles; insufficient consideration given to the specific criteria of suitability for analysis by a candidate; and the tendency to emphasize oedipal pathology while overlooking relevant ego capacities.", "contents": "Some problems in faculty selection of patients for supervised psychoanalysis. The selection of patients for supervised analysis by candidates involves special tasks in evaluation and prediction. A number of problems emerge when the entire faculty of an institute participates in evaluating such patients. These include the different ways in which the initial consultant and the Admitting Psychoanalyst view their roles; insufficient consideration given to the specific criteria of suitability for analysis by a candidate; and the tendency to emphasize oedipal pathology while overlooking relevant ego capacities.", "PMID": 959436}
{"id": "pubmed23n0032_3411", "title": "A psychoanalytic model for human freedom and rationality.", "content": "The nature and scope of freedom and rationality in man are explored in light of the problems posed by a deterministic framework for understanding and explaining human though, feeling, and behavior. It is argued that the sort of explanation afforded by a psychodynamic theory is fully compatible with attributing freedom and rationality to persons. In particular, psychoanalytic theory is able to account for the existence of causal laws governing all aspects of human behavior, while providing a schema by which we can distinguish rational from irrational behavior, and free acts from those that are unfree.", "contents": "A psychoanalytic model for human freedom and rationality. The nature and scope of freedom and rationality in man are explored in light of the problems posed by a deterministic framework for understanding and explaining human though, feeling, and behavior. It is argued that the sort of explanation afforded by a psychodynamic theory is fully compatible with attributing freedom and rationality to persons. In particular, psychoanalytic theory is able to account for the existence of causal laws governing all aspects of human behavior, while providing a schema by which we can distinguish rational from irrational behavior, and free acts from those that are unfree.", "PMID": 959437}
{"id": "pubmed23n0032_3412", "title": "Sex differences in sympathetic-adrenal medullary reactions induced by different stressors.", "content": "Male and female university students were exposed to two different stressors in each of two 110-min sessions, i.e., a cognitive task (color-word conflict) and repeated venipuncture. Catecholamine excretion, heart rate, and subjective reactions were  measured. Control values were obtained under conditions of relaxation in the laboratory. Subjects of both sexes responded to both stressors by increased heart rate and feelings of unpleasantness and distress. The pattern of adrenaline excretion, however, differed between sexes: in males both stressors induced a significant increase, whereas in females adrenaline excretion remained on the same level under the two stress conditions as during relaxation; Noradrenaline excretion was not systematically affected by either stressor in either sex group.", "contents": "Sex differences in sympathetic-adrenal medullary reactions induced by different stressors. Male and female university students were exposed to two different stressors in each of two 110-min sessions, i.e., a cognitive task (color-word conflict) and repeated venipuncture. Catecholamine excretion, heart rate, and subjective reactions were  measured. Control values were obtained under conditions of relaxation in the laboratory. Subjects of both sexes responded to both stressors by increased heart rate and feelings of unpleasantness and distress. The pattern of adrenaline excretion, however, differed between sexes: in males both stressors induced a significant increase, whereas in females adrenaline excretion remained on the same level under the two stress conditions as during relaxation; Noradrenaline excretion was not systematically affected by either stressor in either sex group.", "PMID": 959463}
{"id": "pubmed23n0032_3413", "title": "Hypothalamic chemostimulation and autonomic changes in curarized rats.", "content": "Rats previously implanted with chronic double walled cannulae aimed at the lateral hypothalamic area (LHA) ate and drank reliably after minute injections of norepinephrine and carbachol respectively. Later on the rats were curarized and artificially respirated. After an habituation period during which rectal temperature, cardiac rate and peripheral vasomotor activity were continuously recorded, half of the subjects were injected with 1 mul of norepinephrine (40 x 10(-9) moles) and the other half with 1 mul of carbachol (2.4 x 10(-9)moles). Both drugs elicited hypothermia, bradycardia and vasodilatation. Bradycardia after carbachol was significantly greater than after norepinephrine and hypothermia and vasodilatation after norepinephrine were significantly greater than after carbachol. When the treatments were reversed essentially the same effects were observed.", "contents": "Hypothalamic chemostimulation and autonomic changes in curarized rats. Rats previously implanted with chronic double walled cannulae aimed at the lateral hypothalamic area (LHA) ate and drank reliably after minute injections of norepinephrine and carbachol respectively. Later on the rats were curarized and artificially respirated. After an habituation period during which rectal temperature, cardiac rate and peripheral vasomotor activity were continuously recorded, half of the subjects were injected with 1 mul of norepinephrine (40 x 10(-9) moles) and the other half with 1 mul of carbachol (2.4 x 10(-9)moles). Both drugs elicited hypothermia, bradycardia and vasodilatation. Bradycardia after carbachol was significantly greater than after norepinephrine and hypothermia and vasodilatation after norepinephrine were significantly greater than after carbachol. When the treatments were reversed essentially the same effects were observed.", "PMID": 959464}
{"id": "pubmed23n0032_3414", "title": "Decreased intracranial self-stimulation after neuroleptics or 6-hydroxydopamine: evidence for mediation by motor deficits rather than by reduced reward.", "content": "Rats were implanted with electrodes in the lateral hypothalamus, put on a 22-h food deprivation schedule and trained to bar-press for ICS and for food on a CRF schedule. Haloperidol (0.08 mg/kg) and pimozide (0.22 mg/kg) significantly decreased responding for both reinforces, although responding for ICS was decreased more than it was for food. The same doses of these drugs did not decrease food consumption of a 15-min ad libitum test after 22-h of food deprivation, suggesting that the decreased bar-pressing for food was not the result of anorexia or reduced motivation for food. When similar rates of responding for ICS and for food were obtained on a V1 60 schedule, haloperidol (0.1 mg/kg) reduced responding for food and ICS to a similar extent. Thus, when baseline rate is controlled for, neuroleptics do not selectively reduce responding for ICS. In addition, examination of cumulative response records revealed that rather than producing an extinction curve, as would be predicted if neuroleptics reduced the rewarding properties of ICS, haloperidol produced a uniform decrease in the rate of responding throughout the experimental  session. Similar results were obtained with intraventricular 6-hydroxydopamine (6-OHDA) injections. While these experiments do not exclude the possibility that dopaminergic (DA) systems participate in some central reinforcement mechanisms, they suggest that neuroleptics and 6-OHDA decrease responding for food or ICS primarily by impairing the function of DA systems critically involved in the initiation or maintenance of operant behaviour rather than by interfering with reward.", "contents": "Decreased intracranial self-stimulation after neuroleptics or 6-hydroxydopamine: evidence for mediation by motor deficits rather than by reduced reward. Rats were implanted with electrodes in the lateral hypothalamus, put on a 22-h food deprivation schedule and trained to bar-press for ICS and for food on a CRF schedule. Haloperidol (0.08 mg/kg) and pimozide (0.22 mg/kg) significantly decreased responding for both reinforces, although responding for ICS was decreased more than it was for food. The same doses of these drugs did not decrease food consumption of a 15-min ad libitum test after 22-h of food deprivation, suggesting that the decreased bar-pressing for food was not the result of anorexia or reduced motivation for food. When similar rates of responding for ICS and for food were obtained on a V1 60 schedule, haloperidol (0.1 mg/kg) reduced responding for food and ICS to a similar extent. Thus, when baseline rate is controlled for, neuroleptics do not selectively reduce responding for ICS. In addition, examination of cumulative response records revealed that rather than producing an extinction curve, as would be predicted if neuroleptics reduced the rewarding properties of ICS, haloperidol produced a uniform decrease in the rate of responding throughout the experimental  session. Similar results were obtained with intraventricular 6-hydroxydopamine (6-OHDA) injections. While these experiments do not exclude the possibility that dopaminergic (DA) systems participate in some central reinforcement mechanisms, they suggest that neuroleptics and 6-OHDA decrease responding for food or ICS primarily by impairing the function of DA systems critically involved in the initiation or maintenance of operant behaviour rather than by interfering with reward.", "PMID": 959465}
{"id": "pubmed23n0032_3415", "title": "Effects of drug-state change on discrimination performance.", "content": "A single dose of d-amphetamine (0.25, 0.50, or 1.00 mg/kg), administered in 5 successive sessions, did not seriously impede the discrimination performance of male Holtzman rats under cued reinforcement conditions. A 2.00 mg/kg dose, however, produced a total cessation of operant behavior. In 2 post-drug (saline) sessions, groups previously treated with 0.50 or 1.00 mg/kg demonstrated an initial decrement and subsequent recovery in performance. A second experiment demonstrated that rats administered either saline or 0.50 mg/kg d-amphetamine for 5 successive sessions showed a decrement and subsequent recovery in performance when switched to the opposite treatment condition for the next 2 sessions. These data may be explained in terms of a change in drug state.", "contents": "Effects of drug-state change on discrimination performance. A single dose of d-amphetamine (0.25, 0.50, or 1.00 mg/kg), administered in 5 successive sessions, did not seriously impede the discrimination performance of male Holtzman rats under cued reinforcement conditions. A 2.00 mg/kg dose, however, produced a total cessation of operant behavior. In 2 post-drug (saline) sessions, groups previously treated with 0.50 or 1.00 mg/kg demonstrated an initial decrement and subsequent recovery in performance. A second experiment demonstrated that rats administered either saline or 0.50 mg/kg d-amphetamine for 5 successive sessions showed a decrement and subsequent recovery in performance when switched to the opposite treatment condition for the next 2 sessions. These data may be explained in terms of a change in drug state.", "PMID": 959466}
{"id": "pubmed23n0032_3416", "title": "Salsolinol differentially affects mice selected for sensitivity to alcohol.", "content": "Salsolinol, a compound putatively formed following alcohol ingestion, differentially decreased the activity of lines of mice after 18 generations of genetic selection for alcohol sensitivity. Low doses of salsolinol produced significantly lower activity levels in the alcohol-sensitive long-sleep (LS) line than in the alcohol-insensitive short-sleep (SS) line. A hypnotic dose of salsolinol induced significantly longer sleeptimes in the LS line than in the SS line. Results are interpreted as supporting the hypothesis that salsolinol-like substances may mediate some of the effects of alcohol on the central nervous system.", "contents": "Salsolinol differentially affects mice selected for sensitivity to alcohol. Salsolinol, a compound putatively formed following alcohol ingestion, differentially decreased the activity of lines of mice after 18 generations of genetic selection for alcohol sensitivity. Low doses of salsolinol produced significantly lower activity levels in the alcohol-sensitive long-sleep (LS) line than in the alcohol-insensitive short-sleep (SS) line. A hypnotic dose of salsolinol induced significantly longer sleeptimes in the LS line than in the SS line. Results are interpreted as supporting the hypothesis that salsolinol-like substances may mediate some of the effects of alcohol on the central nervous system.", "PMID": 959467}
{"id": "pubmed23n0032_3417", "title": "Barbital alteration of central nervous system sensitivity to hexobarbital in the rat.", "content": "Alteration in central nervous system (CNS) sensitivity to the hypnotic effect of the hexobarbital was assessed by analytical, electroencephalographic, and pharmacokinetic techniques in barbital-treated rats. Each of these conceptually diverse experimental methodologies yielded similar conclusions concerning the alteration of CNS sensitivity by barbital treatment.", "contents": "Barbital alteration of central nervous system sensitivity to hexobarbital in the rat. Alteration in central nervous system (CNS) sensitivity to the hypnotic effect of the hexobarbital was assessed by analytical, electroencephalographic, and pharmacokinetic techniques in barbital-treated rats. Each of these conceptually diverse experimental methodologies yielded similar conclusions concerning the alteration of CNS sensitivity by barbital treatment.", "PMID": 959468}
{"id": "pubmed23n0032_3418", "title": "Brain acetylcholine concentration and acetylcholinesterase activity in selectively-bred strains of rats.", "content": "Significant differences in whole brain and in brain area acetylcholine concentration have been observed between the two strains of rats selectively bred for differences in speed of acquisition of conditioned avoidance. Brain acetylcholinesterase activity was similar in the strains. The strain showing poor conditioned avoidance behaviour possessed the highest brain concentration of acetylcholine. The finding supports a theory which describes a central cholinergic inhibitory system which may be active in the control of certain types of learning behaviour.", "contents": "Brain acetylcholine concentration and acetylcholinesterase activity in selectively-bred strains of rats. Significant differences in whole brain and in brain area acetylcholine concentration have been observed between the two strains of rats selectively bred for differences in speed of acquisition of conditioned avoidance. Brain acetylcholinesterase activity was similar in the strains. The strain showing poor conditioned avoidance behaviour possessed the highest brain concentration of acetylcholine. The finding supports a theory which describes a central cholinergic inhibitory system which may be active in the control of certain types of learning behaviour.", "PMID": 959469}
{"id": "pubmed23n0032_3419", "title": "The influence of 241-Am and DTPA on morphometric parameters of the rat femur.", "content": "Microradiographically detectable alterations of the bone structure in the femur of young rats induced by monomeric 241-Am(III) (i.v., 30 muCi/kg) were studied. The morphometric and dosimetric measurements were carried out by means of an electronic image analyzer. 8 weeks after injection of 241-Am a characteristic alteration of the frequency distribution of the chord lengths over the trabeculae in the epiphysis and over the metaphyseal marrow spaces was found. The structure of the spongiosa is irregular with both large, coarse and small fragmented trabeculae. The complexity of the bone architecture and the area of the endosteal surfaces is reduced. The surface/volume ratio in control animals varies between 36 mm-1 in the epiphysis and 64 mm-1 in the region of the epiphyseal cartilage plate. From the specific surface burden (pCi/mm2) the average dose rates were determined. There is no significant difference between the calcified tissue fraction in controls and animals with 241-Am, with the exception of the metaphyseal band where the locally high dose rates cause a devitalization of the tissue with inhibition of bone resorption as well as an abnormal trabeculation in the metaphysis. Treatment by Ca-DTPA reduces the 241-Am deposition nonuniformly and the pathological manifestations are markedly less pronounced. The mean trabecular width is about 100 mum in the epiphysis and has a minimum of 40 mum in the central part of the epiphyseal plate. The mean chord length over the marrow spaces varies between 90 and 210 mum.", "contents": "The influence of 241-Am and DTPA on morphometric parameters of the rat femur. Microradiographically detectable alterations of the bone structure in the femur of young rats induced by monomeric 241-Am(III) (i.v., 30 muCi/kg) were studied. The morphometric and dosimetric measurements were carried out by means of an electronic image analyzer. 8 weeks after injection of 241-Am a characteristic alteration of the frequency distribution of the chord lengths over the trabeculae in the epiphysis and over the metaphyseal marrow spaces was found. The structure of the spongiosa is irregular with both large, coarse and small fragmented trabeculae. The complexity of the bone architecture and the area of the endosteal surfaces is reduced. The surface/volume ratio in control animals varies between 36 mm-1 in the epiphysis and 64 mm-1 in the region of the epiphyseal cartilage plate. From the specific surface burden (pCi/mm2) the average dose rates were determined. There is no significant difference between the calcified tissue fraction in controls and animals with 241-Am, with the exception of the metaphyseal band where the locally high dose rates cause a devitalization of the tissue with inhibition of bone resorption as well as an abnormal trabeculation in the metaphysis. Treatment by Ca-DTPA reduces the 241-Am deposition nonuniformly and the pathological manifestations are markedly less pronounced. The mean trabecular width is about 100 mum in the epiphysis and has a minimum of 40 mum in the central part of the epiphyseal plate. The mean chord length over the marrow spaces varies between 90 and 210 mum.", "PMID": 959476}
{"id": "pubmed23n0032_3420", "title": "Heritable chromosome aberrations in mammals after exposure to chemicals.", "content": "The observation of dividing spermatocytes is routinely used to detect the induction of heritable chromosome aberrations such as reciprocal translocations in the treated animals or in their F1 offspring. 37 compounds have so far been tested for the induction of chromosome rearrangements in spermatogonia. Only 9 gave positive results. However, positive results were observed for all alkylating agents in the F1 test. From these observations it can be concluded that the spermatogonia which are the main germ cell type at risk represent a relatively safe germ cell stage.", "contents": "Heritable chromosome aberrations in mammals after exposure to chemicals. The observation of dividing spermatocytes is routinely used to detect the induction of heritable chromosome aberrations such as reciprocal translocations in the treated animals or in their F1 offspring. 37 compounds have so far been tested for the induction of chromosome rearrangements in spermatogonia. Only 9 gave positive results. However, positive results were observed for all alkylating agents in the F1 test. From these observations it can be concluded that the spermatogonia which are the main germ cell type at risk represent a relatively safe germ cell stage.", "PMID": 959475}
{"id": "pubmed23n0032_3421", "title": "Rate constants for nitrification and denitrification in soils.", "content": "Rate constants for reactions in flowing solutions in soil can be calculated from extents of reactions as functions of depth, rates of flow, effective biomass of microbes and independent measurements of hydrodynamic dispersion. Constants have been calculated from data in the literature and are shown to be arbitrary unless all of these quantities have been evaluated. Good agreement of constants obtained in laboratory columns and in the field have been obtained for nitrification and denitrification in a few cases.", "contents": "Rate constants for nitrification and denitrification in soils. Rate constants for reactions in flowing solutions in soil can be calculated from extents of reactions as functions of depth, rates of flow, effective biomass of microbes and independent measurements of hydrodynamic dispersion. Constants have been calculated from data in the literature and are shown to be arbitrary unless all of these quantities have been evaluated. Good agreement of constants obtained in laboratory columns and in the field have been obtained for nitrification and denitrification in a few cases.", "PMID": 959477}
{"id": "pubmed23n0032_3422", "title": "Selective radiolysis of enantiomers.", "content": "The gamma-induced decarboxylation of beta-phenylalanine in the solid state is a chain reaction. Both enantiomers and the racemate of beta-phenylalanine-1-14C show different exponential dose-effect curves for the radiation-induced cleavage of 14CO2.", "contents": "Selective radiolysis of enantiomers. The gamma-induced decarboxylation of beta-phenylalanine in the solid state is a chain reaction. Both enantiomers and the racemate of beta-phenylalanine-1-14C show different exponential dose-effect curves for the radiation-induced cleavage of 14CO2.", "PMID": 959478}
{"id": "pubmed23n0032_3423", "title": "Effects of osmomolarity, calcium and magnesium ions on the structuredness of cytoplasmic matrix (SCM).", "content": "The involvement of intracellular water content, calcium and magnesium ions in changes of the structuredness of cytoplasmic matrix (SCM) in human lymphocytes was investigated with the technique of fluorescence polarization.", "contents": "Effects of osmomolarity, calcium and magnesium ions on the structuredness of cytoplasmic matrix (SCM). The involvement of intracellular water content, calcium and magnesium ions in changes of the structuredness of cytoplasmic matrix (SCM) in human lymphocytes was investigated with the technique of fluorescence polarization.", "PMID": 959479}
{"id": "pubmed23n0032_3424", "title": "Photochemically induced cross-links between DNA and alcohol dehydrogenase or salmine, respectively.", "content": "Model experiments with two structurally different proteins (alcohol dehydrogenase and salmine) show that glycine, alanine, and tyrosine are by far more frequently involved in photochemically induced cross-link formations with DNA than is cysteine. The yields for cross-link formation of thymidine with salmine (cysteine-free) are about as high as those with alcohol dehydrogenase (athiol protein).", "contents": "Photochemically induced cross-links between DNA and alcohol dehydrogenase or salmine, respectively. Model experiments with two structurally different proteins (alcohol dehydrogenase and salmine) show that glycine, alanine, and tyrosine are by far more frequently involved in photochemically induced cross-link formations with DNA than is cysteine. The yields for cross-link formation of thymidine with salmine (cysteine-free) are about as high as those with alcohol dehydrogenase (athiol protein).", "PMID": 959480}
{"id": "pubmed23n0032_3425", "title": "Radiation effects of poly(L-glutamic acid) and poly(L-lysine) in the helix-coil transitional state.", "content": "The degradation of poly(L-glutamic acid) and poly(L-lysine) by X-rays and ultraviolet radiation has been studied at various pH values, including the helix-coil transition region. It was found that X-rays cause maximum degradation in the transition region for both polypeptides, indicating that the efficiency of degradation is affected not only by the secondary structure but also by some other factors related to the transition. On the contrary, the degradation by monochromatic ultraviolet 2537 A radiation showed a strong dependence on helix content of PLGA, suggesting that degradation occurs more effectively in the helical form than in the coil form. Theoretical calculations were made to approximate the pH dependence of degradation, assuming different cross sections for the peptide bonds in helix, coil, and junctions between helical and coil regions.", "contents": "Radiation effects of poly(L-glutamic acid) and poly(L-lysine) in the helix-coil transitional state. The degradation of poly(L-glutamic acid) and poly(L-lysine) by X-rays and ultraviolet radiation has been studied at various pH values, including the helix-coil transition region. It was found that X-rays cause maximum degradation in the transition region for both polypeptides, indicating that the efficiency of degradation is affected not only by the secondary structure but also by some other factors related to the transition. On the contrary, the degradation by monochromatic ultraviolet 2537 A radiation showed a strong dependence on helix content of PLGA, suggesting that degradation occurs more effectively in the helical form than in the coil form. Theoretical calculations were made to approximate the pH dependence of degradation, assuming different cross sections for the peptide bonds in helix, coil, and junctions between helical and coil regions.", "PMID": 959481}
{"id": "pubmed23n0032_3426", "title": "The influence of the counter wall and the counter shape on the spectral energy deposition in small volumes by 60Co gamma-rays and 200 kV x-rays.", "content": "The spectral energy deposition in small volumes by 60Co gamma-rays and by 200 kV X-rays was measured with a walled spherical counter, a walled cylindrical counter and a wall-less cylindrical counter. The simulated volumes had dimensions of 0.1--3 mum. By comparing the spectra of the spherical and cylindrical walled counters the influence of the counter shape is derived. The wall effect is discussed by comparing the spectra and the mean lineal energy measured with the walled and the wall-less cylindrical counters. The influence of the counter size and of radiation quality on the energy deposition spectra is also analysed.", "contents": "The influence of the counter wall and the counter shape on the spectral energy deposition in small volumes by 60Co gamma-rays and 200 kV x-rays. The spectral energy deposition in small volumes by 60Co gamma-rays and by 200 kV X-rays was measured with a walled spherical counter, a walled cylindrical counter and a wall-less cylindrical counter. The simulated volumes had dimensions of 0.1--3 mum. By comparing the spectra of the spherical and cylindrical walled counters the influence of the counter shape is derived. The wall effect is discussed by comparing the spectra and the mean lineal energy measured with the walled and the wall-less cylindrical counters. The influence of the counter size and of radiation quality on the energy deposition spectra is also analysed.", "PMID": 959482}
{"id": "pubmed23n0032_3427", "title": "A nondestructive method for measuring electrical conductivity of intracellular matter of tissue in situ.", "content": "A nondestructive method has been developed for measuring electrical conductivity of intracellular matter. The method is based on easily measurable electric parameters of cells in suspension. Experiments were made using suspensions of erythrocytes in a physiological salt solution. Results confirmed suitability of the method for determining conductance of the cytoplasm and were in close agreement with those reported by other authors. The method allows the determination of intracellular conductivity in tissue as well.", "contents": "A nondestructive method for measuring electrical conductivity of intracellular matter of tissue in situ. A nondestructive method has been developed for measuring electrical conductivity of intracellular matter. The method is based on easily measurable electric parameters of cells in suspension. Experiments were made using suspensions of erythrocytes in a physiological salt solution. Results confirmed suitability of the method for determining conductance of the cytoplasm and were in close agreement with those reported by other authors. The method allows the determination of intracellular conductivity in tissue as well.", "PMID": 959483}
{"id": "pubmed23n0032_3428", "title": "The in vivo effects of 2.45 GHz microwave radiation of rabbit serum components and sleeping times.", "content": "An investigation was conducted to determine the effects of relatively low power density microwave exposures on various serum components of the Dutch rabbit. Both continous wave and pulsed mode exposures at 2.45 GHzwere used at power densities of 25,10 and 5 mW/cm2. Studies of 10 serum components were performed. Additional studies were conducted on changes in sleeping times of pentobarbital-sedated rabbits at various power densities. Gross and histopathological examinations were performed on representative samples of animals. Changes in the blood chemistry of irradiated animals were consistent with a dose-dependent response to a non-specific thermal stress at all power densities used. Observed physiological response, as well as rectal temperature measurements, indicated that the thermoregulatory capability of the rabbits was sufficient to compensate for the thermal burden at 5 and 10 mW/cm2, but could be overridden by a 2 h exposure at 25 mW/cm2. Pathology findings included a mild, repairable nephrosis in animals exposed at a power density of 25 mW/cm2. A further investigation of analeptic effects at power densities varying from 5 mW/cm2 to 50 mW/cm2 resulted in a statistically significant decrease in sleeping times, apparently proportional to power density below 15 mW/cm2.", "contents": "The in vivo effects of 2.45 GHz microwave radiation of rabbit serum components and sleeping times. An investigation was conducted to determine the effects of relatively low power density microwave exposures on various serum components of the Dutch rabbit. Both continous wave and pulsed mode exposures at 2.45 GHzwere used at power densities of 25,10 and 5 mW/cm2. Studies of 10 serum components were performed. Additional studies were conducted on changes in sleeping times of pentobarbital-sedated rabbits at various power densities. Gross and histopathological examinations were performed on representative samples of animals. Changes in the blood chemistry of irradiated animals were consistent with a dose-dependent response to a non-specific thermal stress at all power densities used. Observed physiological response, as well as rectal temperature measurements, indicated that the thermoregulatory capability of the rabbits was sufficient to compensate for the thermal burden at 5 and 10 mW/cm2, but could be overridden by a 2 h exposure at 25 mW/cm2. Pathology findings included a mild, repairable nephrosis in animals exposed at a power density of 25 mW/cm2. A further investigation of analeptic effects at power densities varying from 5 mW/cm2 to 50 mW/cm2 resulted in a statistically significant decrease in sleeping times, apparently proportional to power density below 15 mW/cm2.", "PMID": 959484}
{"id": "pubmed23n0032_3429", "title": "[Pathology of renal tuberculosis (author's transl)].", "content": "Introduction of specific chemotherapy and vaccination leads to a remarkable recession of renal tuberculosis in the younger age group. Renal tuberculosis is the result of a haematogenous spread of tubercle bacillus. Haematogenous spreading occurs immediately after primary infection, or, in elderly patients, in combination with recurrency of tuberculous foci in lungs and hilar-lymphnodes. Simultaneous metastasis in the skeleton, especially in the vertebrae, are observed in 30%. The incubation period between tuberculous spread and clinical manifestation of renal tuberculosis lasts several years, in the average 5-8 years, for calcareous kidneys it may last as long as 20 years and more, for tuberculous pyelitis only a few months. Today it is possible to treat renal tuberculosis with drugs (Streptomycin, PAS and INH). In 13% the cicatrisation is combined with obstruction of calices and partial hydronephrosis, in 7% with obstruction of the ureter and total hydronephrosis. Early chemotherapy may prevent the development of tuberculous hydronephrosis.", "contents": "[Pathology of renal tuberculosis (author's transl)]. Introduction of specific chemotherapy and vaccination leads to a remarkable recession of renal tuberculosis in the younger age group. Renal tuberculosis is the result of a haematogenous spread of tubercle bacillus. Haematogenous spreading occurs immediately after primary infection, or, in elderly patients, in combination with recurrency of tuberculous foci in lungs and hilar-lymphnodes. Simultaneous metastasis in the skeleton, especially in the vertebrae, are observed in 30%. The incubation period between tuberculous spread and clinical manifestation of renal tuberculosis lasts several years, in the average 5-8 years, for calcareous kidneys it may last as long as 20 years and more, for tuberculous pyelitis only a few months. Today it is possible to treat renal tuberculosis with drugs (Streptomycin, PAS and INH). In 13% the cicatrisation is combined with obstruction of calices and partial hydronephrosis, in 7% with obstruction of the ureter and total hydronephrosis. Early chemotherapy may prevent the development of tuberculous hydronephrosis.", "PMID": 959506}
{"id": "pubmed23n0032_3430", "title": "Biological effects of diagnostic irradiation.", "content": "Medical radiation continues to be an area of concern to the patient. However, the technologist can reassure him that trained operators apply the radiation and that there are many built-in and accessory safety factors. Beyond that, however, the technologist can reassure himself that he is not responsible for unnecessary radiation by making full use of all safety measures, whether they are part of or accessory to the equipment, or are part of his judgment as to technique. To do this, he needs a sound knowledge of how damage occurs and a continual updating on research and equipment that will make his reassuring words to the patient honest ones.", "contents": "Biological effects of diagnostic irradiation. Medical radiation continues to be an area of concern to the patient. However, the technologist can reassure him that trained operators apply the radiation and that there are many built-in and accessory safety factors. Beyond that, however, the technologist can reassure himself that he is not responsible for unnecessary radiation by making full use of all safety measures, whether they are part of or accessory to the equipment, or are part of his judgment as to technique. To do this, he needs a sound knowledge of how damage occurs and a continual updating on research and equipment that will make his reassuring words to the patient honest ones.", "PMID": 959501}
{"id": "pubmed23n0032_3431", "title": "[Roentgen diagnosis of renal tuberculosis (author's transl)].", "content": "Roentgenologic procedures are of limited value, compared with other possible examinations. Early radiologic diagnosis is made rarely. Roentgenologic procedures are important for demonstration of successful treatment and for legal documentation. Excretory urography is effective only in combination with zonography and ureteral compression provided the amount of injected contrast medium is sufficient. Angiography is important in progressive or silent renal tuberculosis, especially in cases of a partial or complete non-functioning kidney. Difficulities in differential diagnosis are possible in unspecific pyelonephritis and malignant tumors.", "contents": "[Roentgen diagnosis of renal tuberculosis (author's transl)]. Roentgenologic procedures are of limited value, compared with other possible examinations. Early radiologic diagnosis is made rarely. Roentgenologic procedures are important for demonstration of successful treatment and for legal documentation. Excretory urography is effective only in combination with zonography and ureteral compression provided the amount of injected contrast medium is sufficient. Angiography is important in progressive or silent renal tuberculosis, especially in cases of a partial or complete non-functioning kidney. Difficulities in differential diagnosis are possible in unspecific pyelonephritis and malignant tumors.", "PMID": 959507}
{"id": "pubmed23n0032_3432", "title": "Effect of operator training on patient exposure: an analysis of the NEXT data.", "content": "The Nationwide Evaluation of X-ray Trends (NEXT) program has obtained data from various states on the physical factors related to patient radiation exposure from several common radiographs. Data from two years were examined for exposure, beam area/film area and exposure area product delivered to a \"standard patient.\" The data were examined for differences as a function of operator training.", "contents": "Effect of operator training on patient exposure: an analysis of the NEXT data. The Nationwide Evaluation of X-ray Trends (NEXT) program has obtained data from various states on the physical factors related to patient radiation exposure from several common radiographs. Data from two years were examined for exposure, beam area/film area and exposure area product delivered to a \"standard patient.\" The data were examined for differences as a function of operator training.", "PMID": 959502}
{"id": "pubmed23n0032_3433", "title": "[Angiography of urotuberculosis (author's transl)].", "content": "119 kidneys demonstrating changes consistent with renal tuberculosis were studied in a total of 94 patients by means of selective, transfemoral renal antiography. In the first stage of the disease, this method detected parenchymal involvement which could not be visualized by excretory urography. In stage II the arcuate arteries and occasionally the intralobular arteries, showed typical changes. Cavitation, pathognomonic for the presence of renal tuberculosis were found in 39.3% of these cases. In 40% of the cases the angiographic findingd were more extensive than the apparent findings of excretory urography. Angiography, thus, can render valuable information pertaining to the course of the disease and the necessity of operative intervention. Stage III was usually characterized by extensive changes specific for parenchymal destruction. Typical vascular lesions were readily recognized. Cavitation was found in 86.5% of these cases. Tortuosity and dilatation of the renal pelvis- and/or ureter-arteries revealed evidence of ureteral involvement (stage III2). In the presence of a non-functioning kidney angiography is mandatory to rule out renal aplasia and to differentiate between a kidney destroyed by other disease processes and the complete cavitary destruction of end-stage renal tuberculosis (stage III3).", "contents": "[Angiography of urotuberculosis (author's transl)]. 119 kidneys demonstrating changes consistent with renal tuberculosis were studied in a total of 94 patients by means of selective, transfemoral renal antiography. In the first stage of the disease, this method detected parenchymal involvement which could not be visualized by excretory urography. In stage II the arcuate arteries and occasionally the intralobular arteries, showed typical changes. Cavitation, pathognomonic for the presence of renal tuberculosis were found in 39.3% of these cases. In 40% of the cases the angiographic findingd were more extensive than the apparent findings of excretory urography. Angiography, thus, can render valuable information pertaining to the course of the disease and the necessity of operative intervention. Stage III was usually characterized by extensive changes specific for parenchymal destruction. Typical vascular lesions were readily recognized. Cavitation was found in 86.5% of these cases. Tortuosity and dilatation of the renal pelvis- and/or ureter-arteries revealed evidence of ureteral involvement (stage III2). In the presence of a non-functioning kidney angiography is mandatory to rule out renal aplasia and to differentiate between a kidney destroyed by other disease processes and the complete cavitary destruction of end-stage renal tuberculosis (stage III3).", "PMID": 959508}
{"id": "pubmed23n0032_3434", "title": "[Clinic of Urotuberculosis (author's transl)].", "content": "Most of all cases of extrapulmonary tuberculosis i.e. 35% are urogenital tuberculosis UGT. A shifting of morbidity to older age Groups could be ascertained. 1164 cases were evaluated, 684 in a combined medicamentons - operative manner. Of the conservatively treated Group were 283 males and 197 females; the relation of the sexus in the other group is roughly the same. The frequency has a maximum in the forth decade. Whereas most of 90 patients, who have been in stationary treatment in April 1975, were between 40 and 60 years old. The pathogeny of the UGT is shown according to the scherne of Gloor. Most of the initial Symptoms (26.5%) are dysuria, the mext are hematuriae and ache of the flanks. On an average 1-2 years pass from the first appearance of symtonis to the time of diagnosis. The diagnosis can only be verified by evidence of Tbc-bacteria in cultures and biological experiments or through histological appearance. Essential informations on localisation and expansion are obtained by x-ray examination, that is urograms and urethrocystograms. Angiography and side-disconnectet isotope clearance are importantant additional examinations. According to the results of the x-ray examinations the stages can be ascertained: Stage I: parenchymal-ulcerated pattern - Stage II: ulcerated-cavernous pattern - Stage III: totally-destructing tuberculosis, tbc-pyonephrosis, Special terms characterize the obstructions of the urinary tract. For the therapy the following polity can be proposed: Stage I: chemotherapy - Stage II: chemotherapy, perhaps backed by conservative operative treatment - Stage III: chemotherapy and nephro-ureterektomy.", "contents": "[Clinic of Urotuberculosis (author's transl)]. Most of all cases of extrapulmonary tuberculosis i.e. 35% are urogenital tuberculosis UGT. A shifting of morbidity to older age Groups could be ascertained. 1164 cases were evaluated, 684 in a combined medicamentons - operative manner. Of the conservatively treated Group were 283 males and 197 females; the relation of the sexus in the other group is roughly the same. The frequency has a maximum in the forth decade. Whereas most of 90 patients, who have been in stationary treatment in April 1975, were between 40 and 60 years old. The pathogeny of the UGT is shown according to the scherne of Gloor. Most of the initial Symptoms (26.5%) are dysuria, the mext are hematuriae and ache of the flanks. On an average 1-2 years pass from the first appearance of symtonis to the time of diagnosis. The diagnosis can only be verified by evidence of Tbc-bacteria in cultures and biological experiments or through histological appearance. Essential informations on localisation and expansion are obtained by x-ray examination, that is urograms and urethrocystograms. Angiography and side-disconnectet isotope clearance are importantant additional examinations. According to the results of the x-ray examinations the stages can be ascertained: Stage I: parenchymal-ulcerated pattern - Stage II: ulcerated-cavernous pattern - Stage III: totally-destructing tuberculosis, tbc-pyonephrosis, Special terms characterize the obstructions of the urinary tract. For the therapy the following polity can be proposed: Stage I: chemotherapy - Stage II: chemotherapy, perhaps backed by conservative operative treatment - Stage III: chemotherapy and nephro-ureterektomy.", "PMID": 959509}
{"id": "pubmed23n0032_3435", "title": "[Generalized skeletal dysplasias (author's transl)].", "content": "Skeletal Dysplasias are an important cause of short stature. Their overall frequency is unknown; their individual number exceeds 80. Prognostic and genetic counseling depend on a correct diagnosis of the specific bone dysplasia. Specific radiographic diagnoses are also needed for the meaningful interpretation of forthcoming histological, ultrastructural and biochemical studies which will help to elucidate the pathogenesis of skeletal dysplasias as prerequisite for a causal therapy.", "contents": "[Generalized skeletal dysplasias (author's transl)]. Skeletal Dysplasias are an important cause of short stature. Their overall frequency is unknown; their individual number exceeds 80. Prognostic and genetic counseling depend on a correct diagnosis of the specific bone dysplasia. Specific radiographic diagnoses are also needed for the meaningful interpretation of forthcoming histological, ultrastructural and biochemical studies which will help to elucidate the pathogenesis of skeletal dysplasias as prerequisite for a causal therapy.", "PMID": 959510}
{"id": "pubmed23n0032_3436", "title": "[Which films should be taken? Suspicion for congenital skeletal dysplasia (author's transl)].", "content": "Clinical signs and roentgenologic alterations are important for classification of congenital skeletal dysplasis. It is summarized which skeletal parts are most important for the roentgenographic determination of bone dysplasia. A minimal survey includes films of the vertebral column (lateral projection of the pelvis, hand, knee and skull (lateral projection). A schematic presentation demonstrates the vertebral column lesions in the most important congenital skeletal dysplasias.", "contents": "[Which films should be taken? Suspicion for congenital skeletal dysplasia (author's transl)]. Clinical signs and roentgenologic alterations are important for classification of congenital skeletal dysplasis. It is summarized which skeletal parts are most important for the roentgenographic determination of bone dysplasia. A minimal survey includes films of the vertebral column (lateral projection of the pelvis, hand, knee and skull (lateral projection). A schematic presentation demonstrates the vertebral column lesions in the most important congenital skeletal dysplasias.", "PMID": 959511}
{"id": "pubmed23n0032_3437", "title": "Advisory committees: free advice--for a price.", "content": "Although it is understood that an advisory committee is \"a group of persons outside the educational institution chosen from the community to advise those who are responsible for the planning, implementing, and maintaining of an educational program in radiologic technology that will meet the needs of a community, region, state, or nation,\" it is advice we buy at a price. No, the price is not in monetary values, but the price of forethought, planning, good organization and effective use of that committee.", "contents": "Advisory committees: free advice--for a price. Although it is understood that an advisory committee is \"a group of persons outside the educational institution chosen from the community to advise those who are responsible for the planning, implementing, and maintaining of an educational program in radiologic technology that will meet the needs of a community, region, state, or nation,\" it is advice we buy at a price. No, the price is not in monetary values, but the price of forethought, planning, good organization and effective use of that committee.", "PMID": 959505}
{"id": "pubmed23n0032_3438", "title": "[Importance of hand films in skeletal dysplasia (author's transl)].", "content": "The hand, as part of the skeleton, is generally involved in systemic skeletal dysplasia. However, the degree of abnormalities differs considerably in the various types of dysplasia. In some, abnormal appearance of the hand predominates, in other dysplasia films of the hand provide only little or no useful diagnostic information. At their first examination for growth disorders children often reveal bone dysplasia on roentgenograms of the hand. Therefore, evaluation of hand films should be done as thoroughly as possible. Morphologic and/or metric deviations of the hand may bring the first suspicion although they are not fully diagnostic. Systemic skeletal dysplasia should be diagnosed or excluded by additional adequate radiologic and other clinical examinations.", "contents": "[Importance of hand films in skeletal dysplasia (author's transl)]. The hand, as part of the skeleton, is generally involved in systemic skeletal dysplasia. However, the degree of abnormalities differs considerably in the various types of dysplasia. In some, abnormal appearance of the hand predominates, in other dysplasia films of the hand provide only little or no useful diagnostic information. At their first examination for growth disorders children often reveal bone dysplasia on roentgenograms of the hand. Therefore, evaluation of hand films should be done as thoroughly as possible. Morphologic and/or metric deviations of the hand may bring the first suspicion although they are not fully diagnostic. Systemic skeletal dysplasia should be diagnosed or excluded by additional adequate radiologic and other clinical examinations.", "PMID": 959512}
{"id": "pubmed23n0032_3439", "title": "[The so-called chondrodystrophia of the newborn (author's transl)].", "content": "Chondrodystrophia (now: Achondroplasia) formerly believed to be a uniform disease rather is an entity comprising up till now at least ten radiologically differing types. Definite diagnosis of these types can only be made radiologically although certain clinical features are of additional help. For practical purpose a schematic representation of these types is given, based upon the classification of congenital osteochondrodysplasias by the ESPR (European Society of Pediatric Radiology, Rome, 1969). It emphasizes the characteristic radiological signs and describes the typical changes in body proportions. In a second part the single diseases are discussed according to the scheme with their radiological differential diagnosis and their prominent changes in the skeletal system. Some examples are given for possibly associated malformations and final prognosis.", "contents": "[The so-called chondrodystrophia of the newborn (author's transl)]. Chondrodystrophia (now: Achondroplasia) formerly believed to be a uniform disease rather is an entity comprising up till now at least ten radiologically differing types. Definite diagnosis of these types can only be made radiologically although certain clinical features are of additional help. For practical purpose a schematic representation of these types is given, based upon the classification of congenital osteochondrodysplasias by the ESPR (European Society of Pediatric Radiology, Rome, 1969). It emphasizes the characteristic radiological signs and describes the typical changes in body proportions. In a second part the single diseases are discussed according to the scheme with their radiological differential diagnosis and their prominent changes in the skeletal system. Some examples are given for possibly associated malformations and final prognosis.", "PMID": 959513}
{"id": "pubmed23n0032_3440", "title": "[Radiological diagnosis (and differential diagnosis) of lethal type (congenital type) of hypophosphatasia (author's transl)].", "content": "Diagnosis of Hypophosphatasia can be proved by typical radiological findings. The paper presents a case report of a newborn living only a few hours. Diagnosis according to clinical aspect was \"Osteogenesis imperfecta\" although a postmortal x-ray-babygram was available but which had not been shown to a radiologist. Anatomical diagnosis with the histological findings was then altered to \"Metaphyseal chondrodysplasia, Jansen-type\". Finally the diagnosis could be corrected to congenital, lethal type of Hypophosphatasia after the babygram had been shown to a radiologist. The typical radiological features of congenital Hypophosphatasia are described subsequently and differential diagnosis of Congenital Osteochondrodysplasias is discussed. The importance of early or postmortal diagnosis for genetic counselling is stressed as well as a close cooperation between obstetricians and pediatric radiologists. Typical biochemical findings are mentioned.", "contents": "[Radiological diagnosis (and differential diagnosis) of lethal type (congenital type) of hypophosphatasia (author's transl)]. Diagnosis of Hypophosphatasia can be proved by typical radiological findings. The paper presents a case report of a newborn living only a few hours. Diagnosis according to clinical aspect was \"Osteogenesis imperfecta\" although a postmortal x-ray-babygram was available but which had not been shown to a radiologist. Anatomical diagnosis with the histological findings was then altered to \"Metaphyseal chondrodysplasia, Jansen-type\". Finally the diagnosis could be corrected to congenital, lethal type of Hypophosphatasia after the babygram had been shown to a radiologist. The typical radiological features of congenital Hypophosphatasia are described subsequently and differential diagnosis of Congenital Osteochondrodysplasias is discussed. The importance of early or postmortal diagnosis for genetic counselling is stressed as well as a close cooperation between obstetricians and pediatric radiologists. Typical biochemical findings are mentioned.", "PMID": 959514}
{"id": "pubmed23n0032_3441", "title": "[Measurement of the absorbed dose to the patient by computerized whole-body x-ray tomography with the aid of thermoluminescence dosimeters in an Alderson-phantom (author's transl)].", "content": "Radiation dosis for brain, lung, liver, and kidney examinations which were performed with computerized wholebody scanner (Ohio-Nuclear, 120 KV, 30 mA) was measured by LiF-TLD in the Alderson-Phantom. The absorbed skin dosis to the trunk by a single scan (filtration 6 mm A1) was found to be 1.2 rd at the entrance and 90 mrd at the exit. Patient investigations were simulated with a series of 5 scans. Under those conditions the maximum abdominal entrance dosis is 1.8 rd, at the body axis 700 mrd and near the exit 250 mrd. In this example the dosis to the gonads is less than 2 and 4 mrd, if the distance is more than 15 cm between the last scan and the gonads. Scattered radiation in the Delta-Scan room during an investigation is 1.5 mR/scan and 0.3 mR/scan for a distance of 1 m and 2 m from the irradiated volume.", "contents": "[Measurement of the absorbed dose to the patient by computerized whole-body x-ray tomography with the aid of thermoluminescence dosimeters in an Alderson-phantom (author's transl)]. Radiation dosis for brain, lung, liver, and kidney examinations which were performed with computerized wholebody scanner (Ohio-Nuclear, 120 KV, 30 mA) was measured by LiF-TLD in the Alderson-Phantom. The absorbed skin dosis to the trunk by a single scan (filtration 6 mm A1) was found to be 1.2 rd at the entrance and 90 mrd at the exit. Patient investigations were simulated with a series of 5 scans. Under those conditions the maximum abdominal entrance dosis is 1.8 rd, at the body axis 700 mrd and near the exit 250 mrd. In this example the dosis to the gonads is less than 2 and 4 mrd, if the distance is more than 15 cm between the last scan and the gonads. Scattered radiation in the Delta-Scan room during an investigation is 1.5 mR/scan and 0.3 mR/scan for a distance of 1 m and 2 m from the irradiated volume.", "PMID": 959516}
{"id": "pubmed23n0032_3442", "title": "[Ultrasonic diagnosis in diseases of kidneys (author's transl)].", "content": "The diagnostic value of echoscopy and echography in kidney disease is presented and illustrated. Ultrasound examination is primarily used for the differentiation of cystic and solid lesions of the kidney. Our personal experience and the recent literature on this problem is reviewed. Based on the overall diagnostic accuracy of more than 90%, a procedure to investigate space occupying lesions of the kidney is suggested. The indication for ultrasound scans in obstructive kidney disease and the unilateral nonexcreting kidney is briefly discussed.", "contents": "[Ultrasonic diagnosis in diseases of kidneys (author's transl)]. The diagnostic value of echoscopy and echography in kidney disease is presented and illustrated. Ultrasound examination is primarily used for the differentiation of cystic and solid lesions of the kidney. Our personal experience and the recent literature on this problem is reviewed. Based on the overall diagnostic accuracy of more than 90%, a procedure to investigate space occupying lesions of the kidney is suggested. The indication for ultrasound scans in obstructive kidney disease and the unilateral nonexcreting kidney is briefly discussed.", "PMID": 959517}
{"id": "pubmed23n0032_3443", "title": "[Gray-scale ultrasongraphy of the liver and gallbladder (author's transl)].", "content": "Better structural imaging of the liver with blood vessels can be achieved using the Gray-scale technique which delineates pathological processes better than the conventional B-scan-method. This technique enables a reliable diagnosic information. Due to its non-invasive character it can be utilized as a screening method or as an alternative or complementary examination to invasive methods.", "contents": "[Gray-scale ultrasongraphy of the liver and gallbladder (author's transl)]. Better structural imaging of the liver with blood vessels can be achieved using the Gray-scale technique which delineates pathological processes better than the conventional B-scan-method. This technique enables a reliable diagnosic information. Due to its non-invasive character it can be utilized as a screening method or as an alternative or complementary examination to invasive methods.", "PMID": 959518}
{"id": "pubmed23n0032_3444", "title": "Echography of suprarenal masses.", "content": "Ultrasound is used in evaluation of suprarenal rumors. Results are compared with angiographic and surgical findings. A practical approach is proposed.", "contents": "Echography of suprarenal masses. Ultrasound is used in evaluation of suprarenal rumors. Results are compared with angiographic and surgical findings. A practical approach is proposed.", "PMID": 959519}
{"id": "pubmed23n0032_3445", "title": "[Importance of sonotomography in radiation therapy (author's transl)].", "content": "Ultrasound tomography provides true scale representation of body contours and organ structures. The image supplies substantial, individual geometrical data, essential for computerized radiation treatment planning. The mehtod is described. Typical planning examples for therapy are demonstrated. The value of follow up sonograms for radiation therapy is described. The limitations of the method are pointed out.", "contents": "[Importance of sonotomography in radiation therapy (author's transl)]. Ultrasound tomography provides true scale representation of body contours and organ structures. The image supplies substantial, individual geometrical data, essential for computerized radiation treatment planning. The mehtod is described. Typical planning examples for therapy are demonstrated. The value of follow up sonograms for radiation therapy is described. The limitations of the method are pointed out.", "PMID": 959520}
{"id": "pubmed23n0032_3446", "title": "[Examination of the breast with ultrasound: limits and possibilities (authro's transl)].", "content": "Following a brief introduction to ultrasound the advantages and disadvantages of this examination are presented with regard to differentiation of benign and malignant tumors of the breast. This method is unsuitable for screening because of its present technical failures: its only importance lies in the differential diagnosis of benign lesions. These statements are based upon the case material of the Department of Gynecology and Radiology of the Postgraduate Medical School of Budapest.", "contents": "[Examination of the breast with ultrasound: limits and possibilities (authro's transl)]. Following a brief introduction to ultrasound the advantages and disadvantages of this examination are presented with regard to differentiation of benign and malignant tumors of the breast. This method is unsuitable for screening because of its present technical failures: its only importance lies in the differential diagnosis of benign lesions. These statements are based upon the case material of the Department of Gynecology and Radiology of the Postgraduate Medical School of Budapest.", "PMID": 959521}
{"id": "pubmed23n0032_3447", "title": "The vagus reaction as a possible cause of severe complications of radiological procedures.", "content": "Six severe reactions which appeared to be mediated via the vagus nerve were encountered in the course of various radiological procedures. Four patients were treated with intravenous atropine and responded successfully. The vagus reaction is proposed as a possible mechanism of death in the \"allergic\" reaction to contrast media seen during radiological procedures. This phenomenon and its treatment and prevention are discussed, as is its difference from the histamine effect.", "contents": "The vagus reaction as a possible cause of severe complications of radiological procedures. Six severe reactions which appeared to be mediated via the vagus nerve were encountered in the course of various radiological procedures. Four patients were treated with intravenous atropine and responded successfully. The vagus reaction is proposed as a possible mechanism of death in the \"allergic\" reaction to contrast media seen during radiological procedures. This phenomenon and its treatment and prevention are discussed, as is its difference from the histamine effect.", "PMID": 959522}
{"id": "pubmed23n0032_3448", "title": "Localized spinal cord atrophy: significance of its demonstration.", "content": "Localized spinal cord atrophy was demonstrated in 6 cases by gas myelography. All of the patients had neck injuries with subsequent impairment of neurological function. Three representative cases are reported in full, and the remainder tabulated. The significance of the demonstration of this condition is discussed.", "contents": "Localized spinal cord atrophy: significance of its demonstration. Localized spinal cord atrophy was demonstrated in 6 cases by gas myelography. All of the patients had neck injuries with subsequent impairment of neurological function. Three representative cases are reported in full, and the remainder tabulated. The significance of the demonstration of this condition is discussed.", "PMID": 959523}
{"id": "pubmed23n0032_3449", "title": "Brain scanning with the Anger multiplane tomographic scanner as a second examination evaluation by the ROC method.", "content": "One hundred and one patients (43 abnormal, 58 normal) were re-examined with a commercial prototype of the Anger multiplane tomographic scanner after brain scintigraphy. The studies were interpreted independently by four observers of varying experience. Observer performance, in terms of ROC curves, was substantially better when interpreting the tomographic brain scans and camera scintigrams together or the tomographic scans alone than when interpreting the camera images alone. Re-examination with the scanner increases the accuracy of brain scintigraphy (observer performance) in cases in which there is difficulty distinguishing intracranial lesions from superficial foci of activity by camera scintigraphy alone.", "contents": "Brain scanning with the Anger multiplane tomographic scanner as a second examination evaluation by the ROC method. One hundred and one patients (43 abnormal, 58 normal) were re-examined with a commercial prototype of the Anger multiplane tomographic scanner after brain scintigraphy. The studies were interpreted independently by four observers of varying experience. Observer performance, in terms of ROC curves, was substantially better when interpreting the tomographic brain scans and camera scintigrams together or the tomographic scans alone than when interpreting the camera images alone. Re-examination with the scanner increases the accuracy of brain scintigraphy (observer performance) in cases in which there is difficulty distinguishing intracranial lesions from superficial foci of activity by camera scintigraphy alone.", "PMID": 959524}
{"id": "pubmed23n0032_3450", "title": "Brain scanning with the Anger multiplane tomographic scanner as a primary examination. Evaluation by the ROC method.", "content": "A commercial prototype of the Anger multiplane tomographic scanner was compared with a scintillation camera, in terms of observer performance, as a primary instrument for brain scintigraphy. Observer performance (diagnostic accuracy), expressed as ROC curves, was slightly better when reading the tomographic scans than when reading the camera images. In brain scintigraphy of a general population, the scanner has a small advantage over the scintillation camera. This advantage is directly proportional to the fraction of cases in which there is difficulty differentiating intracranial lesions from superficial foci of activity on the camera image.", "contents": "Brain scanning with the Anger multiplane tomographic scanner as a primary examination. Evaluation by the ROC method. A commercial prototype of the Anger multiplane tomographic scanner was compared with a scintillation camera, in terms of observer performance, as a primary instrument for brain scintigraphy. Observer performance (diagnostic accuracy), expressed as ROC curves, was slightly better when reading the tomographic scans than when reading the camera images. In brain scintigraphy of a general population, the scanner has a small advantage over the scintillation camera. This advantage is directly proportional to the fraction of cases in which there is difficulty differentiating intracranial lesions from superficial foci of activity on the camera image.", "PMID": 959525}
{"id": "pubmed23n0032_3451", "title": "Myocardial imaging with radionuclide-labeled particles. Analysis of the normal image, abnormal image, and technical consideration.", "content": "Myocardial imaging following the direct injection of labeled particles is  an effective and safe method of studying regional perfusion. Perfusion defects can conveniently be classified as apical, anterior, inferior, or posterior and related to the distribution of specific coronary arteries. The hemodynamic effects of contrast material, inadequate mixing of particles, coronary anatomy, and associated cardiac pathology are important technical considerations affecting the performance and interpretation of studies using this technique.", "contents": "Myocardial imaging with radionuclide-labeled particles. Analysis of the normal image, abnormal image, and technical consideration. Myocardial imaging following the direct injection of labeled particles is  an effective and safe method of studying regional perfusion. Perfusion defects can conveniently be classified as apical, anterior, inferior, or posterior and related to the distribution of specific coronary arteries. The hemodynamic effects of contrast material, inadequate mixing of particles, coronary anatomy, and associated cardiac pathology are important technical considerations affecting the performance and interpretation of studies using this technique.", "PMID": 959526}
{"id": "pubmed23n0032_3452", "title": "Persisting perfusion defects after bronchoscopic removal or spontaneous expulsion of aspirated foreign objects.", "content": "Follow-up lung scans were obtained in 9 children to determine whether perfusion lung scanning could be used to verify completeness of foreign-body removal. In 4 patients, extensive perfusion defects were present 4-19 days after removal of the object. In 1 patient, the area of hypoperfusion was larger than the territory served by a persistently obstructed lobar bronchus. Follow-up lung scans are of limited value for verifying completeness of foreign-body removal and localization of retained fragments.", "contents": "Persisting perfusion defects after bronchoscopic removal or spontaneous expulsion of aspirated foreign objects. Follow-up lung scans were obtained in 9 children to determine whether perfusion lung scanning could be used to verify completeness of foreign-body removal. In 4 patients, extensive perfusion defects were present 4-19 days after removal of the object. In 1 patient, the area of hypoperfusion was larger than the territory served by a persistently obstructed lobar bronchus. Follow-up lung scans are of limited value for verifying completeness of foreign-body removal and localization of retained fragments.", "PMID": 959527}
{"id": "pubmed23n0032_3453", "title": "Upper airway obstruction in infants and small children. Improved radiographic diagnosis by combining filtration, high kilovoltage, and magnification.", "content": "Selective filtration, high kilovoltage, and fine focal spot magnification produce detailed radiographs of the airway of infants and small children. This technique has been applied to the study of upper airway obstruction in the glottic, subglottic, and supraglottic areas. Its great diagnostic yield is accompanied by a reduction in radiation dose compared to that of a nonfilter technique. The physical factors dictating choice of filter and film-screen combination are discussed.", "contents": "Upper airway obstruction in infants and small children. Improved radiographic diagnosis by combining filtration, high kilovoltage, and magnification. Selective filtration, high kilovoltage, and fine focal spot magnification produce detailed radiographs of the airway of infants and small children. This technique has been applied to the study of upper airway obstruction in the glottic, subglottic, and supraglottic areas. Its great diagnostic yield is accompanied by a reduction in radiation dose compared to that of a nonfilter technique. The physical factors dictating choice of filter and film-screen combination are discussed.", "PMID": 959528}
{"id": "pubmed23n0032_3454", "title": "Antegonial notching of the mandible: an often overlooked mandibular deformity in congenital and acquired disorders.", "content": "Mandibular antegonial notching is a concavity of the undersurface of the body just anterior to the angular process (gonion) seen in congenital and acquired disorders. The notch tends to be longer in the congenital than in the acquired state, and the ascending ramus is at a more obtuse angle to the body.", "contents": "Antegonial notching of the mandible: an often overlooked mandibular deformity in congenital and acquired disorders. Mandibular antegonial notching is a concavity of the undersurface of the body just anterior to the angular process (gonion) seen in congenital and acquired disorders. The notch tends to be longer in the congenital than in the acquired state, and the ascending ramus is at a more obtuse angle to the body.", "PMID": 959529}
{"id": "pubmed23n0032_3455", "title": "Vertebral sarcoidosis in adolescents.", "content": "Two cases of vertebral sarcoidosis in pediatric patients are presented. All such patients reported have been black, 13-15 years old, and have a history of back pain. Radiographs of the involved vertebrae show primarily lytic destruction with sclerotic borders in some of the lesions. Fungal infections, tuberculosis, pyogenic osteomyelitis, Hodgkin's disease and metastatic disease must be considered in every patient with vertebral sarcoidosis.", "contents": "Vertebral sarcoidosis in adolescents. Two cases of vertebral sarcoidosis in pediatric patients are presented. All such patients reported have been black, 13-15 years old, and have a history of back pain. Radiographs of the involved vertebrae show primarily lytic destruction with sclerotic borders in some of the lesions. Fungal infections, tuberculosis, pyogenic osteomyelitis, Hodgkin's disease and metastatic disease must be considered in every patient with vertebral sarcoidosis.", "PMID": 959530}
{"id": "pubmed23n0032_3456", "title": "Papillary necrosis in a child with homozygous sickle-cell anemia.", "content": "A 4 1/2-year-old girl with S-S sickle-cell anemia was found to have papillary necrosis on excretory urography. This is believed to be the first reported case of papillary necrosis in a child with homozygous S-S sickle-cell anemia.", "contents": "Papillary necrosis in a child with homozygous sickle-cell anemia. A 4 1/2-year-old girl with S-S sickle-cell anemia was found to have papillary necrosis on excretory urography. This is believed to be the first reported case of papillary necrosis in a child with homozygous S-S sickle-cell anemia.", "PMID": 959531}
{"id": "pubmed23n0032_3457", "title": "Gray scale shifts in ultrasound displays.", "content": "Shifts in the displayed shades of gray were found in the initial evaluation of a gray scale ultrasound display. Quantitative measurements of these shifts were performed. These measurements, along with measurements of scan converter output and photographic exposure time, established television (TV) performance as the major cause of shifts. Random variations in photographic exposure time were shown to cause small random shifts. Recommendations are given to minimize the effects of these shifts in clinical sonograms. To minimize shifts, hardware modifications were made on a similar TV. These modifications substantially improved the TV performance.", "contents": "Gray scale shifts in ultrasound displays. Shifts in the displayed shades of gray were found in the initial evaluation of a gray scale ultrasound display. Quantitative measurements of these shifts were performed. These measurements, along with measurements of scan converter output and photographic exposure time, established television (TV) performance as the major cause of shifts. Random variations in photographic exposure time were shown to cause small random shifts. Recommendations are given to minimize the effects of these shifts in clinical sonograms. To minimize shifts, hardware modifications were made on a similar TV. These modifications substantially improved the TV performance.", "PMID": 959532}
{"id": "pubmed23n0032_3458", "title": "A reappraisal of sonolucent renal masses.", "content": "The authors present a differential diagnosis of echo-free renal masses and emphasize that such lesions are not always simple cysts. Of 312 renal sonograms, 15 showed sonolucent masses which proved to be something other than a simple cyst. Pathological diagnoses included abscess, hematoma, renal artery aneurysm, hydronephrosis, and homogeneous or cystic tumor. To achieve optimal diagnostic accuracy, it is insufficient to rely solely on sonolucency as demonstrated on the sonogram. Any disparities in the radiological, clinical, or ultrasonic findings demand further evaluation. Nephrotomography, angiography, aspiration biopsy, or exploration should be selected in a sequence which is appropriate to ensure correct diagnosis while minimizing the risk to the patient.", "contents": "A reappraisal of sonolucent renal masses. The authors present a differential diagnosis of echo-free renal masses and emphasize that such lesions are not always simple cysts. Of 312 renal sonograms, 15 showed sonolucent masses which proved to be something other than a simple cyst. Pathological diagnoses included abscess, hematoma, renal artery aneurysm, hydronephrosis, and homogeneous or cystic tumor. To achieve optimal diagnostic accuracy, it is insufficient to rely solely on sonolucency as demonstrated on the sonogram. Any disparities in the radiological, clinical, or ultrasonic findings demand further evaluation. Nephrotomography, angiography, aspiration biopsy, or exploration should be selected in a sequence which is appropriate to ensure correct diagnosis while minimizing the risk to the patient.", "PMID": 959533}
{"id": "pubmed23n0032_3459", "title": "Inflammatory carcinoma of the breast.", "content": "Eighty-six patients with the clinical features of inflammatory carcinoma of the breast (erythema, peau d'orange, wheals or ridges) were treated with irradiation for potential cure between July 1948 and December 1970. Long protracted irradiation alone with a strong skin reaction offers 50% local control, which is the best rate yet reported. Any surgical procedure beyond biopsy is probably damaging: all patients subjected to mastectomy developed distant metastases and died. Ninety per cent of the patients were dead by five years and only 3 patients remain alive without evidence of disease 7, 10 and 14 years after radiotherapy. Analysis of survival rates, incidence, sites and times of appearance of local recurrences, and distant metastases is presented.", "contents": "Inflammatory carcinoma of the breast. Eighty-six patients with the clinical features of inflammatory carcinoma of the breast (erythema, peau d'orange, wheals or ridges) were treated with irradiation for potential cure between July 1948 and December 1970. Long protracted irradiation alone with a strong skin reaction offers 50% local control, which is the best rate yet reported. Any surgical procedure beyond biopsy is probably damaging: all patients subjected to mastectomy developed distant metastases and died. Ninety per cent of the patients were dead by five years and only 3 patients remain alive without evidence of disease 7, 10 and 14 years after radiotherapy. Analysis of survival rates, incidence, sites and times of appearance of local recurrences, and distant metastases is presented.", "PMID": 959534}
{"id": "pubmed23n0032_3460", "title": "Endodermal sinus tumors of the ovary in children. The role of radiation therapy in relation to the clinical course.", "content": "Endodermal sinus tumor of the ovary in children has been characterized by its general resistance to therapy and its almost uniform fatality. The multidisciplinary treatment of 5 such cases is described. The author's experience suggests that these tumors are responsive to irradiation. The rationale for a combined approach is discussed, and radiation treatment volumes and doses are suggested.", "contents": "Endodermal sinus tumors of the ovary in children. The role of radiation therapy in relation to the clinical course. Endodermal sinus tumor of the ovary in children has been characterized by its general resistance to therapy and its almost uniform fatality. The multidisciplinary treatment of 5 such cases is described. The author's experience suggests that these tumors are responsive to irradiation. The rationale for a combined approach is discussed, and radiation treatment volumes and doses are suggested.", "PMID": 959535}
{"id": "pubmed23n0032_3461", "title": "The effect of prednisone and irradiation on the rectum in dogs.", "content": "In an attempt to diminish the severity of the acute and late effects of irradiation to the rectum of dogs, oral prednisone was administered to 10 dogs for 1 week prior to, during, and for 1 month following a 3-week fractionated course of 60Co exposures to the pelvis. A control group of 10 dogs received irradiation alone. The dogs were observed clinically, serial rectal biopsies were done during and following the acute reaction, and the rectum was studied following sacrifice. Observations suggest that prednisone has no beneficial effect on the acute inflammatory reaction, and increases the severity of the late tissue damage.", "contents": "The effect of prednisone and irradiation on the rectum in dogs. In an attempt to diminish the severity of the acute and late effects of irradiation to the rectum of dogs, oral prednisone was administered to 10 dogs for 1 week prior to, during, and for 1 month following a 3-week fractionated course of 60Co exposures to the pelvis. A control group of 10 dogs received irradiation alone. The dogs were observed clinically, serial rectal biopsies were done during and following the acute reaction, and the rectum was studied following sacrifice. Observations suggest that prednisone has no beneficial effect on the acute inflammatory reaction, and increases the severity of the late tissue damage.", "PMID": 959536}
{"id": "pubmed23n0032_3462", "title": "Visualization of differences in soft-tissue densities. The liver in ascites.", "content": "The lateral liver border was demonstrated on the plain radiograph in 54 of 100 proved cases of ascites. The etiology of ascites, the density of the fluid, and the total protein content were similar whether the border was visualized or not. Tomography of the right upper quadrant indicated that the lateral liver margin is visualized in ascites as a result of contact between the liver and the surrounding fluid. Radiography of autopsied livers submerged in fluid demonstrated distinguishable differences in object density, and photodensitometry of the plain radiographs revealed a difference in blackness where the fluid and liver made contact. Comparison of mean liver density and mean ascitic fluid density showed a difference of 5%. These findings indicate that differences in soft-tissue densities (excluding fat) can be distinguished on the plain radiograph.", "contents": "Visualization of differences in soft-tissue densities. The liver in ascites. The lateral liver border was demonstrated on the plain radiograph in 54 of 100 proved cases of ascites. The etiology of ascites, the density of the fluid, and the total protein content were similar whether the border was visualized or not. Tomography of the right upper quadrant indicated that the lateral liver margin is visualized in ascites as a result of contact between the liver and the surrounding fluid. Radiography of autopsied livers submerged in fluid demonstrated distinguishable differences in object density, and photodensitometry of the plain radiographs revealed a difference in blackness where the fluid and liver made contact. Comparison of mean liver density and mean ascitic fluid density showed a difference of 5%. These findings indicate that differences in soft-tissue densities (excluding fat) can be distinguished on the plain radiograph.", "PMID": 959538}
{"id": "pubmed23n0032_3463", "title": "Electronic grids for electrostatic imaging systems.", "content": "Electronic grids have been substituted for conventional grids in an electrostatic imaging system and have been useful in chest radiography. Electronic grids also appear to be useful for clean-up of small amounts of scattered radiation in radiographs of the extremities. More clinical work is needed before a statement about the use of electronic grids for other examinations can be made.", "contents": "Electronic grids for electrostatic imaging systems. Electronic grids have been substituted for conventional grids in an electrostatic imaging system and have been useful in chest radiography. Electronic grids also appear to be useful for clean-up of small amounts of scattered radiation in radiographs of the extremities. More clinical work is needed before a statement about the use of electronic grids for other examinations can be made.", "PMID": 959539}
{"id": "pubmed23n0032_3464", "title": "Direct chronotropic and dromotropic actions of contrast media: ineffectiveness of atropine in the prevention of bradyarrhythmias and conduction disturbances.", "content": "Methyl glucamine sodium diatrizoate (Renografin 76) was directly infused into the isolated arteries to the sino-atrial and atrioventricular nodes of anesthetized dogs, causing a dose-related inhibition of sino-atrial nodal automaticity and atrioventricular nodal conductivity; these effects were not attenuated by autonomic blockade or cervical vagotomy. The important negative chronotropic and dramatropic effects of contrast media used for coronary angiography are direct actions and cannot be effectively blocked by premedication with atropine.", "contents": "Direct chronotropic and dromotropic actions of contrast media: ineffectiveness of atropine in the prevention of bradyarrhythmias and conduction disturbances. Methyl glucamine sodium diatrizoate (Renografin 76) was directly infused into the isolated arteries to the sino-atrial and atrioventricular nodes of anesthetized dogs, causing a dose-related inhibition of sino-atrial nodal automaticity and atrioventricular nodal conductivity; these effects were not attenuated by autonomic blockade or cervical vagotomy. The important negative chronotropic and dramatropic effects of contrast media used for coronary angiography are direct actions and cannot be effectively blocked by premedication with atropine.", "PMID": 959540}
{"id": "pubmed23n0032_3465", "title": "The diagnostic quality of optically processed radiographs.", "content": "The diagnostic quality of optically processed radiographs was compared with that of unprocessed radiographs, using the number of lesions detected as a criterion. It was found that merely removing phase-shifted information with either a binary or focal spot filter alone did not improve image quality, however, use of the focal spot filter plus removal of all phase-shifted frequencies did lead to the detection of an increased number of lesions. The authors suggest that currently available optical filtration techniques should be evaluated with caution, as they may actually degrade diagnostic quality by increasing the visibility of phase-shifted information.", "contents": "The diagnostic quality of optically processed radiographs. The diagnostic quality of optically processed radiographs was compared with that of unprocessed radiographs, using the number of lesions detected as a criterion. It was found that merely removing phase-shifted information with either a binary or focal spot filter alone did not improve image quality, however, use of the focal spot filter plus removal of all phase-shifted frequencies did lead to the detection of an increased number of lesions. The authors suggest that currently available optical filtration techniques should be evaluated with caution, as they may actually degrade diagnostic quality by increasing the visibility of phase-shifted information.", "PMID": 959541}
{"id": "pubmed23n0032_3466", "title": "Film mammography: new low radiation technology.", "content": "A new mammographic technique with Kodak MIN-R film and a Kodak MIN-R screen in a vacuum package has been evaluated in 607 cases. Direct mammographic comparison is made with the Du Pont Lo-dose system in the first 101 patients. The system provides excellent diagnostic quality images with at least a two-fold reduction in patient exposure compared to other current mammographic techniques.", "contents": "Film mammography: new low radiation technology. A new mammographic technique with Kodak MIN-R film and a Kodak MIN-R screen in a vacuum package has been evaluated in 607 cases. Direct mammographic comparison is made with the Du Pont Lo-dose system in the first 101 patients. The system provides excellent diagnostic quality images with at least a two-fold reduction in patient exposure compared to other current mammographic techniques.", "PMID": 959542}
{"id": "pubmed23n0032_3467", "title": "Percutaneous transhepatic cholangiography utilizing the Chiba University needle.", "content": "Percutaneous transhepatic cholangiography with the Chiba University needle has been performed in 42 patients with cholestasis. The examination was successful in 100% of patients with dilated intrahepatic bile ducts and in 89% of those with normal-size ducts. Complications were minimal. The results of this study suggest that PTC with the Chiba needle is superior to the current conventional methods. The patients with intrahepatic cholestasis, which often simulates extrahepatic biliary obstruction, were spared an unnecessary laparotomy.", "contents": "Percutaneous transhepatic cholangiography utilizing the Chiba University needle. Percutaneous transhepatic cholangiography with the Chiba University needle has been performed in 42 patients with cholestasis. The examination was successful in 100% of patients with dilated intrahepatic bile ducts and in 89% of those with normal-size ducts. Complications were minimal. The results of this study suggest that PTC with the Chiba needle is superior to the current conventional methods. The patients with intrahepatic cholestasis, which often simulates extrahepatic biliary obstruction, were spared an unnecessary laparotomy.", "PMID": 959543}
{"id": "pubmed23n0032_3468", "title": "Opinion: Caveat doctor: the x-ray collimator industry takes a giant step backward.", "content": "Markedly improved imaging of the upper airway in infants and children with magnification high kVp technique is accomplished with added heavy filtration. Unfortunately, in the newer x-ray collimators, no slot is provided for this purpose. The authors can think of no reason, medical, physical or economic, as to why a simple slot cannot be provided. A few manufactures make machines with the added filter slot; all radiologists are urged not to neglect this information when selecting new equipment.", "contents": "Opinion: Caveat doctor: the x-ray collimator industry takes a giant step backward. Markedly improved imaging of the upper airway in infants and children with magnification high kVp technique is accomplished with added heavy filtration. Unfortunately, in the newer x-ray collimators, no slot is provided for this purpose. The authors can think of no reason, medical, physical or economic, as to why a simple slot cannot be provided. A few manufactures make machines with the added filter slot; all radiologists are urged not to neglect this information when selecting new equipment.", "PMID": 959546}
{"id": "pubmed23n0032_3469", "title": "Subpulmonic pneumothorax in patients with chronic obstructive pulmonary disease.", "content": "A spontaneous pneumothorax is occasionally seen under the lung on upright views, apparently due to subpulmonic trapping of pleural air by adhesions. This is most likely to occur in patients with chronic obstructive pulmonary disease (COPD), especially if they have extensive parenchymal scarring from previous tuberculosis. The authors describe 4 patients who had subpulmonic pneumothorax, severe COPD, and parenchymal scarring and presented in respiratory failure. None had other clinical symptoms suggesting pneumothorax. Radiologists should be aware of this condition, since it is potentially lethal.", "contents": "Subpulmonic pneumothorax in patients with chronic obstructive pulmonary disease. A spontaneous pneumothorax is occasionally seen under the lung on upright views, apparently due to subpulmonic trapping of pleural air by adhesions. This is most likely to occur in patients with chronic obstructive pulmonary disease (COPD), especially if they have extensive parenchymal scarring from previous tuberculosis. The authors describe 4 patients who had subpulmonic pneumothorax, severe COPD, and parenchymal scarring and presented in respiratory failure. None had other clinical symptoms suggesting pneumothorax. Radiologists should be aware of this condition, since it is potentially lethal.", "PMID": 959548}
{"id": "pubmed23n0032_3470", "title": "Post-traumatic pneumatocele in the inferior pulmonary ligament.", "content": "Pneumatocele localized to the inferior pulmonary ligament is an uncommon sequel of blunt chest trauma. Three such cases are reported and the characteristic location and appearance of the pneumatocele, as well as its benign course, are discussed. A similar radiographic appearance has been produced in cadavers by injecting air into the inferior pulmonary ligament.", "contents": "Post-traumatic pneumatocele in the inferior pulmonary ligament. Pneumatocele localized to the inferior pulmonary ligament is an uncommon sequel of blunt chest trauma. Three such cases are reported and the characteristic location and appearance of the pneumatocele, as well as its benign course, are discussed. A similar radiographic appearance has been produced in cadavers by injecting air into the inferior pulmonary ligament.", "PMID": 959549}
{"id": "pubmed23n0032_3471", "title": "Thoracic manifestations of renal cell carcinoma.", "content": "Over 1/3 of patients with renal cell carcinoma will present with metastases with no symptoms referable to the kidney. Early metastatic disease is a result of the unique accessibility of the kidney to lymphohematogenous pathways. The thorax is particularly vulnerable. Several of the more unusual manifestations involving the thorax are illustrated, including 1 case with intra-alveolar metastases.", "contents": "Thoracic manifestations of renal cell carcinoma. Over 1/3 of patients with renal cell carcinoma will present with metastases with no symptoms referable to the kidney. Early metastatic disease is a result of the unique accessibility of the kidney to lymphohematogenous pathways. The thorax is particularly vulnerable. Several of the more unusual manifestations involving the thorax are illustrated, including 1 case with intra-alveolar metastases.", "PMID": 959550}
{"id": "pubmed23n0032_3472", "title": "Bilateral pulmonary hilar lymphadenopathy. An unusual manifestation of metastatic renal cell carcinoma.", "content": "Four patients with bilateral pulmonary hilar adenopathy secondary to lymphangitic spread from renal cell carcinoma were examined. Two additional cases had adenopathy secondary to nasopharyngeal carcinoma. Patients may initially present with bilateral pulmonary lymphadenopathy or as late as 3 1/2 years after the discovery of the primary renal tumor. The mechanism of lymphangitic spread probably is related to reflux of tumor emboli from the thoracic duct into the bronchomediastinal trunks because of incompetent lymphatic valves. In one case gallium imaging demonstrated bilateral hilar isotopic uptake as well as periaortic uptake.", "contents": "Bilateral pulmonary hilar lymphadenopathy. An unusual manifestation of metastatic renal cell carcinoma. Four patients with bilateral pulmonary hilar adenopathy secondary to lymphangitic spread from renal cell carcinoma were examined. Two additional cases had adenopathy secondary to nasopharyngeal carcinoma. Patients may initially present with bilateral pulmonary lymphadenopathy or as late as 3 1/2 years after the discovery of the primary renal tumor. The mechanism of lymphangitic spread probably is related to reflux of tumor emboli from the thoracic duct into the bronchomediastinal trunks because of incompetent lymphatic valves. In one case gallium imaging demonstrated bilateral hilar isotopic uptake as well as periaortic uptake.", "PMID": 959551}
{"id": "pubmed23n0032_3473", "title": "Bradycardia and hypotension following use of intravenous contrast media.", "content": "Eight patients had unusual reactions, including hypotension and bradycardia, to intravenous contrast media. These reactions appear to be vagal- or acetylcholine-mediated. Atropine treatment produced prompt reversal in several of the cases, and is felt to be the drug of choice for this particular type of idiosyncratic reaction.", "contents": "Bradycardia and hypotension following use of intravenous contrast media. Eight patients had unusual reactions, including hypotension and bradycardia, to intravenous contrast media. These reactions appear to be vagal- or acetylcholine-mediated. Atropine treatment produced prompt reversal in several of the cases, and is felt to be the drug of choice for this particular type of idiosyncratic reaction.", "PMID": 959552}
{"id": "pubmed23n0032_3474", "title": "Intravascular electrically induced thrombosis in the dog. Thrombus detection by ionic and nonionic contrast media.", "content": "Reliable, large, nonobstructive thrombi were produced by electrical stimulation of the wall of the inferior caval veins in dogs, thus avoiding surgical trauma or introduction of chemical substances. Metrizamide was found to adhere longer to the thrombi than an ionic medium (iothalamate) at comparable iodine concentrations. This may be due to the much higher viscosity of metrizamide.", "contents": "Intravascular electrically induced thrombosis in the dog. Thrombus detection by ionic and nonionic contrast media. Reliable, large, nonobstructive thrombi were produced by electrical stimulation of the wall of the inferior caval veins in dogs, thus avoiding surgical trauma or introduction of chemical substances. Metrizamide was found to adhere longer to the thrombi than an ionic medium (iothalamate) at comparable iodine concentrations. This may be due to the much higher viscosity of metrizamide.", "PMID": 959553}
{"id": "pubmed23n0032_3475", "title": "Diagnosis and pathophysiology of paradoxical embolism.", "content": "Three cases of paradoxical embolism are presented. Although most systemic emboli originate in the heart, detection of other sources is aided by venography of the lower extremities, indocyanine green-dye curve studies of the inferior vena cava, obtaining right heart pressures, oximetry, and pulmonary angiography. Forty per cent of 55 patients reviewed had repeat episodes of systemic embolism and 2 of the 3 proved cases of paradoxical embolism had repeat episodes. Prolonged anticoagulant therapy may prevent recurrence.", "contents": "Diagnosis and pathophysiology of paradoxical embolism. Three cases of paradoxical embolism are presented. Although most systemic emboli originate in the heart, detection of other sources is aided by venography of the lower extremities, indocyanine green-dye curve studies of the inferior vena cava, obtaining right heart pressures, oximetry, and pulmonary angiography. Forty per cent of 55 patients reviewed had repeat episodes of systemic embolism and 2 of the 3 proved cases of paradoxical embolism had repeat episodes. Prolonged anticoagulant therapy may prevent recurrence.", "PMID": 959554}
{"id": "pubmed23n0032_3476", "title": "Idiopathic nonfamilial acro-osteolysis with cortical defects and mandibular ramus osteolysis.", "content": "A case is presented of progressive acro-osteolysis of the hands combined with progressive mandibular ramus osteolysis and multiple cortical defects. The features of acro-osteolysis are reviewed and classified.", "contents": "Idiopathic nonfamilial acro-osteolysis with cortical defects and mandibular ramus osteolysis. A case is presented of progressive acro-osteolysis of the hands combined with progressive mandibular ramus osteolysis and multiple cortical defects. The features of acro-osteolysis are reviewed and classified.", "PMID": 959555}
{"id": "pubmed23n0032_3477", "title": "Right axillary lymph node visualization by bipedal lymphography: a case report.", "content": "In the course of bipedal lymphography, the right axillary nodes were filled with contrast medium from the right pelvic region via the subcutaneous lymphatics. This was verified in the absence of lymphatic obstruction or abnormal corollary lymphatic routes.", "contents": "Right axillary lymph node visualization by bipedal lymphography: a case report. In the course of bipedal lymphography, the right axillary nodes were filled with contrast medium from the right pelvic region via the subcutaneous lymphatics. This was verified in the absence of lymphatic obstruction or abnormal corollary lymphatic routes.", "PMID": 959556}
{"id": "pubmed23n0032_3478", "title": "Radiopaque suppositories.", "content": "The authors describe the radiographic appearance of several commonly used rectal suppositories. Such drugs are sufficiently opaque to be seen on abdominal radiographs and may simulate excreted urographic contrast medium or stones. Errors in interpretation can be avoided by asking the patient about rectal medication or by taking lateral or delayed films.", "contents": "Radiopaque suppositories. The authors describe the radiographic appearance of several commonly used rectal suppositories. Such drugs are sufficiently opaque to be seen on abdominal radiographs and may simulate excreted urographic contrast medium or stones. Errors in interpretation can be avoided by asking the patient about rectal medication or by taking lateral or delayed films.", "PMID": 959557}
{"id": "pubmed23n0032_3479", "title": "Regional enteritis complicated by nephrocalcinosis and nephrolithiasis. Case report.", "content": "The authors report a case of regional enteritis complicated by nephrocalcinosis and nephrolithiasis. Enteric hyperoxaluria is documented and the pathophysiology is discussed.", "contents": "Regional enteritis complicated by nephrocalcinosis and nephrolithiasis. Case report. The authors report a case of regional enteritis complicated by nephrocalcinosis and nephrolithiasis. Enteric hyperoxaluria is documented and the pathophysiology is discussed.", "PMID": 959558}
{"id": "pubmed23n0032_3480", "title": "Angiographic demonstration of a calcified splenic hamartoma.", "content": "A case of hamartoma of the spleen is reported. Radiographic examination revealed a mass containing innumerable calcifications in the left upper quadrant. Angiography demonstrated an avascular mass. The authors believe that such calcification and avascularity are late manifestations of a slowly-growing splenic tumor which may well be hypervascular if examined earlier.", "contents": "Angiographic demonstration of a calcified splenic hamartoma. A case of hamartoma of the spleen is reported. Radiographic examination revealed a mass containing innumerable calcifications in the left upper quadrant. Angiography demonstrated an avascular mass. The authors believe that such calcification and avascularity are late manifestations of a slowly-growing splenic tumor which may well be hypervascular if examined earlier.", "PMID": 959559}
{"id": "pubmed23n0032_3481", "title": "Contrast enhancement of cerebral infarcts in computed tomography.", "content": "Approximately 60% of cerebral infarcts between 1 and 4 weeks old may be enhanced by contrast material. A recent infarct can be missed if contrast material is not used; however, recent infarcts can also be partially obscured by the contrast medium, so that a precontrast scan is necessary. Infarcts, arteriovenous malformations, and gliomas may appear similar on the CT scan. Recent infarcts can have a significant mass effect (seen in 22% of cases), and their anatomical location and morphology may or may not be helpful in the differential diagnosis. Ipsilateral ventricular dilatation, homogeneous low-density areas, and sharp margins are usually found in older infarcts.", "contents": "Contrast enhancement of cerebral infarcts in computed tomography. Approximately 60% of cerebral infarcts between 1 and 4 weeks old may be enhanced by contrast material. A recent infarct can be missed if contrast material is not used; however, recent infarcts can also be partially obscured by the contrast medium, so that a precontrast scan is necessary. Infarcts, arteriovenous malformations, and gliomas may appear similar on the CT scan. Recent infarcts can have a significant mass effect (seen in 22% of cases), and their anatomical location and morphology may or may not be helpful in the differential diagnosis. Ipsilateral ventricular dilatation, homogeneous low-density areas, and sharp margins are usually found in older infarcts.", "PMID": 959561}
{"id": "pubmed23n0032_3482", "title": "Mach bands and density perception.", "content": "Perception of a roentgen image is greatly influenced by the production of Mach bands by the retinal neural networks. The mechanism of their production and contributing factors such as lateral inhibition, projection, contour, film density, object density, and background are discussed. Although Mach bands often facilitate perception of roentgen density, misinterpretation of their significance may lead to errors in diagnosis.", "contents": "Mach bands and density perception. Perception of a roentgen image is greatly influenced by the production of Mach bands by the retinal neural networks. The mechanism of their production and contributing factors such as lateral inhibition, projection, contour, film density, object density, and background are discussed. Although Mach bands often facilitate perception of roentgen density, misinterpretation of their significance may lead to errors in diagnosis.", "PMID": 959562}
{"id": "pubmed23n0032_3483", "title": "Quantification of bone mineralization using computed tomography.", "content": "Computed tomography was used to find a sensitive parameter for bone mineralization. A precision scanning instrument was constructed for determination of the mineral distribution in sections of the forearm. The quality of the reconstructed images allows separate quantification of compact and spongy bone even when gamma rays are used. Computer simulation and measured of models and macerated human bones showed that under clinical conditions it is possible to quantify spongy bone density within an accuracy of +/-2%.", "contents": "Quantification of bone mineralization using computed tomography. Computed tomography was used to find a sensitive parameter for bone mineralization. A precision scanning instrument was constructed for determination of the mineral distribution in sections of the forearm. The quality of the reconstructed images allows separate quantification of compact and spongy bone even when gamma rays are used. Computer simulation and measured of models and macerated human bones showed that under clinical conditions it is possible to quantify spongy bone density within an accuracy of +/-2%.", "PMID": 959563}
{"id": "pubmed23n0032_3484", "title": "Radiographic anatomy of the accessory meningeal artery.", "content": "The accessory meningeal artery (AMA) was studied in 6 cadavers and correlated with the angiographic findings in 50 normal patients. The AMA is easily identified angiographically, its origin varying with the course of the internal maxillary artery. The authors believe that all intracranial lesions which are not strictly intracerebral should be investigated by means of selective injection of the internal maxillary artery.", "contents": "Radiographic anatomy of the accessory meningeal artery. The accessory meningeal artery (AMA) was studied in 6 cadavers and correlated with the angiographic findings in 50 normal patients. The AMA is easily identified angiographically, its origin varying with the course of the internal maxillary artery. The authors believe that all intracranial lesions which are not strictly intracerebral should be investigated by means of selective injection of the internal maxillary artery.", "PMID": 959564}
{"id": "pubmed23n0032_3485", "title": "A connection between the production of prostaglandins during liver regeneration and the DNA synthetic response.", "content": "The concentration of PGE equivalents in rat liver in vivo was increased during liver regeneration. The probability that this increase reflected a stimulation of prostaglandin synthesis was confirmed in vitro by the ability of homogenates of regenerating liver tissue to synthesize PGE2 and PGF2alpha from arachidonate. Indomethacin prevented these prostaglandin changes, and the subsequent increase in DNA synthesis. The administration of indomethacin to rats was found to induce a hypocalcemia, and to block the hypercalcemic action of parathyroid hormone. It is suggested that the influence of prostaglandins on DNA synthesis involves calcium metabolism.", "contents": "A connection between the production of prostaglandins during liver regeneration and the DNA synthetic response. The concentration of PGE equivalents in rat liver in vivo was increased during liver regeneration. The probability that this increase reflected a stimulation of prostaglandin synthesis was confirmed in vitro by the ability of homogenates of regenerating liver tissue to synthesize PGE2 and PGF2alpha from arachidonate. Indomethacin prevented these prostaglandin changes, and the subsequent increase in DNA synthesis. The administration of indomethacin to rats was found to induce a hypocalcemia, and to block the hypercalcemic action of parathyroid hormone. It is suggested that the influence of prostaglandins on DNA synthesis involves calcium metabolism.", "PMID": 959565}
{"id": "pubmed23n0032_3486", "title": "Defective PGE reactivity in leucocytes of multiple sclerosis patients.", "content": "Leucocyte migration inhibition in vitro, in response to antigen or mitogen, is suppressed by PGE2 (0.01-1.0 mug/ml). The susceptibility of leucocytes to such inhibition by PGE2 has been compared using cell preparations obtained from normal individuals, multiple sclerosis patients and from patients with other neurological diseases. The results indicate defective reactivity of leucocytes from multiple sclerosis patients.", "contents": "Defective PGE reactivity in leucocytes of multiple sclerosis patients. Leucocyte migration inhibition in vitro, in response to antigen or mitogen, is suppressed by PGE2 (0.01-1.0 mug/ml). The susceptibility of leucocytes to such inhibition by PGE2 has been compared using cell preparations obtained from normal individuals, multiple sclerosis patients and from patients with other neurological diseases. The results indicate defective reactivity of leucocytes from multiple sclerosis patients.", "PMID": 959566}
{"id": "pubmed23n0032_3487", "title": "Effect of topical indomethacin on UVR-induced redness and prostaglandin E levels in sunburned guinea pig skin.", "content": "Prostaglandin E (PGE) levels in the skin have been shown to be elevated during the 24 hr. period following exposure of guinea pig skin to ultraviolet radiation from 280-320 nm in the so-called UVB or \"sunburn spectrum\". The development of increased PGE levels paralleled the development of the delayed phase of erythema. When applied immediately after UVB exposure, one topical application of 2.5% Indomethacin (IM) simultaneously decreased the redness and PGE levels in the skin to near normal within one hr. of treatment. The IM effect persisted for approximately 24 hrs. By 48 hrs. the PGE level in the sunburned skin was near normal whether or not the skin had been treated with IM. The redness which was evident at 48 hrs. did not respond to additional topical IM treatment. This suggests that a substance(s) other than PGE may be involved in this latter portion of UVB-induced erythema. A major role for PGE as an early mediator or potentiator of UVB-induced erythema has been deomonstrated. Any involvement of PGE with UVB-induced cell death or alterations in DNA synthesis seems unlikely.", "contents": "Effect of topical indomethacin on UVR-induced redness and prostaglandin E levels in sunburned guinea pig skin. Prostaglandin E (PGE) levels in the skin have been shown to be elevated during the 24 hr. period following exposure of guinea pig skin to ultraviolet radiation from 280-320 nm in the so-called UVB or \"sunburn spectrum\". The development of increased PGE levels paralleled the development of the delayed phase of erythema. When applied immediately after UVB exposure, one topical application of 2.5% Indomethacin (IM) simultaneously decreased the redness and PGE levels in the skin to near normal within one hr. of treatment. The IM effect persisted for approximately 24 hrs. By 48 hrs. the PGE level in the sunburned skin was near normal whether or not the skin had been treated with IM. The redness which was evident at 48 hrs. did not respond to additional topical IM treatment. This suggests that a substance(s) other than PGE may be involved in this latter portion of UVB-induced erythema. A major role for PGE as an early mediator or potentiator of UVB-induced erythema has been deomonstrated. Any involvement of PGE with UVB-induced cell death or alterations in DNA synthesis seems unlikely.", "PMID": 959567}
{"id": "pubmed23n0032_3488", "title": "Prostaglandin removal and metabolism by isolated perfused rat lung.", "content": "We have investigated the mechanism(s) involved in the removal of prostaglandins (PG) from the pulmonary circulation by the lung. Unidirectional fluxes of PG from the circulation into the lung are measured in an isolated perfused rat lung preparation. Evidence is presented which suggests that a transport system for PG exists in lung tissue. This transport system is responsible for the removal of some PG from the circulation by the lung. PGE1 and PGF2alpha are substrates for this system, whereas PGB1, PGA1, and 15-keto-PGF2alpha are not. Since PGA1 is a substrate for the intracellular PG dehydrogenase, the selectivity of the lung's metabolism system for circulating PG is probably due to the selectivity of the transport system for PG. It is shown that the percentage of the pulmonary arterial concentration (CA) of PGE1 or PGF2alpha that is metabolized on passage through the pulmonary circulation decreases rapidly as CA increases. When the lungs were perfused with PGE1(PGF2alpha), the metabolites detected in the venous effluent were 15-keto-PGE1 (PGF2alpha) and 15-keto-13, 14-dihydro-PGE1 (PGF2alpha). The time course pattern of the appearance of metabolites in the venous effluent after the initiation of a constant CA, and the relative concentrations of the metabolites in the venous effluent, were examined as a function of CA.", "contents": "Prostaglandin removal and metabolism by isolated perfused rat lung. We have investigated the mechanism(s) involved in the removal of prostaglandins (PG) from the pulmonary circulation by the lung. Unidirectional fluxes of PG from the circulation into the lung are measured in an isolated perfused rat lung preparation. Evidence is presented which suggests that a transport system for PG exists in lung tissue. This transport system is responsible for the removal of some PG from the circulation by the lung. PGE1 and PGF2alpha are substrates for this system, whereas PGB1, PGA1, and 15-keto-PGF2alpha are not. Since PGA1 is a substrate for the intracellular PG dehydrogenase, the selectivity of the lung's metabolism system for circulating PG is probably due to the selectivity of the transport system for PG. It is shown that the percentage of the pulmonary arterial concentration (CA) of PGE1 or PGF2alpha that is metabolized on passage through the pulmonary circulation decreases rapidly as CA increases. When the lungs were perfused with PGE1(PGF2alpha), the metabolites detected in the venous effluent were 15-keto-PGE1 (PGF2alpha) and 15-keto-13, 14-dihydro-PGE1 (PGF2alpha). The time course pattern of the appearance of metabolites in the venous effluent after the initiation of a constant CA, and the relative concentrations of the metabolites in the venous effluent, were examined as a function of CA.", "PMID": 959568}
{"id": "pubmed23n0032_3489", "title": "Modification of alveolar hyperoxia induced pulmonary vasodilatation by indomethacin.", "content": "Decrease in pulmonary vascular resistance was observed in neonatal minature pigs breathing 100% O2 or 95% O2:5% CO2. The pulmonary vasodilator response to hyperoxia ventilation was reduced by indomethacin in the intact animal and in the isolated perfused lung preparation. In the isolated perfused lung preparation, it was also shown that lung alveolar pO2 rather than pulmonary arterial pO2 was responsible for the pulmonary vasodilation. The study suggests that alveolar hyperoxia induced decrease in pulmonary vascular resistance may be mediated in part by release of prostaglandins. The relevance of this study with oxygen therapy in newborn infants is also discussed.", "contents": "Modification of alveolar hyperoxia induced pulmonary vasodilatation by indomethacin. Decrease in pulmonary vascular resistance was observed in neonatal minature pigs breathing 100% O2 or 95% O2:5% CO2. The pulmonary vasodilator response to hyperoxia ventilation was reduced by indomethacin in the intact animal and in the isolated perfused lung preparation. In the isolated perfused lung preparation, it was also shown that lung alveolar pO2 rather than pulmonary arterial pO2 was responsible for the pulmonary vasodilation. The study suggests that alveolar hyperoxia induced decrease in pulmonary vascular resistance may be mediated in part by release of prostaglandins. The relevance of this study with oxygen therapy in newborn infants is also discussed.", "PMID": 959569}
{"id": "pubmed23n0032_3490", "title": "Inhibition of prostaglandin F2alpha-induced reflex bradycardia and hypotension by meclofenamic acid.", "content": "Intravenous injection of prostaglandin F2alpha (4-15 mug/kg, i.v.) produces an increase in pulmonary arterial pressure in conjunction with reflex bradycardia and hypotension in the anesthetized cat. Meclofenamic acid (30 mg/kg, i.v.) inhibited the bradycardia and the reflex contribution to the systemic hypotension. Neither the PGF2alpha-induced pulmonary vasoconstriction nor the direct systemic vasodilator actions of PGF2alpha were blocked by meclofenamate. In addition, the reflex responses caused by i.v. veratrine and 5-HT were not inhibited by meclofenamate. These results suggest that meclofenamic acid selectively blocks the afferent mechanism by which PGF2alpha induces reflex bradycardia and hypotension in the cat.", "contents": "Inhibition of prostaglandin F2alpha-induced reflex bradycardia and hypotension by meclofenamic acid. Intravenous injection of prostaglandin F2alpha (4-15 mug/kg, i.v.) produces an increase in pulmonary arterial pressure in conjunction with reflex bradycardia and hypotension in the anesthetized cat. Meclofenamic acid (30 mg/kg, i.v.) inhibited the bradycardia and the reflex contribution to the systemic hypotension. Neither the PGF2alpha-induced pulmonary vasoconstriction nor the direct systemic vasodilator actions of PGF2alpha were blocked by meclofenamate. In addition, the reflex responses caused by i.v. veratrine and 5-HT were not inhibited by meclofenamate. These results suggest that meclofenamic acid selectively blocks the afferent mechanism by which PGF2alpha induces reflex bradycardia and hypotension in the cat.", "PMID": 959570}
{"id": "pubmed23n0032_3491", "title": "Kinetic and metabolic studies of 15-methyl-prostaglandin F2alpha administered intra-amniotically for induction of abortion.", "content": "The metabolism of tritium labeled 15-methyl-PGF2alpha, administered intra-amniotically, was studied in seven cases of mid-trimester abortion. The disappearance of the compound from the amniotic sac was a very slow process. A metabolite, dinor-15-methyl-PGF2alpha, was found in small amounts in the amniotic and kidney. Plasma levels of 15-methyl-PGF2alpha in the maternal circulation were determined.", "contents": "Kinetic and metabolic studies of 15-methyl-prostaglandin F2alpha administered intra-amniotically for induction of abortion. The metabolism of tritium labeled 15-methyl-PGF2alpha, administered intra-amniotically, was studied in seven cases of mid-trimester abortion. The disappearance of the compound from the amniotic sac was a very slow process. A metabolite, dinor-15-methyl-PGF2alpha, was found in small amounts in the amniotic and kidney. Plasma levels of 15-methyl-PGF2alpha in the maternal circulation were determined.", "PMID": 959571}
{"id": "pubmed23n0032_3492", "title": "Lack of evidence for inhibition of ovulation by aspirin in women.", "content": "Prostaglandin synthetase inhibitors such as aspirin and indomethacin inhibit ovulation in experimental animals. The effect of therapeutic doses of aspirin on ovulation has been studied in 5 women. Ovulation, as assessed by measurements of urinary LH and plasma progesterone levels, basal body temperature and cervical mucus changes occured in all the control and treatment cycles. Serial sections of the corpora lutea failed to show any entrapped ova. The significance of finding a prostaglandin synthetase inhibitor which acts specifically at the ovarian level is discussed.", "contents": "Lack of evidence for inhibition of ovulation by aspirin in women. Prostaglandin synthetase inhibitors such as aspirin and indomethacin inhibit ovulation in experimental animals. The effect of therapeutic doses of aspirin on ovulation has been studied in 5 women. Ovulation, as assessed by measurements of urinary LH and plasma progesterone levels, basal body temperature and cervical mucus changes occured in all the control and treatment cycles. Serial sections of the corpora lutea failed to show any entrapped ova. The significance of finding a prostaglandin synthetase inhibitor which acts specifically at the ovarian level is discussed.", "PMID": 959572}
{"id": "pubmed23n0032_3493", "title": "Effects of indomethacin and prostaglandins on the spawning behaviour of female goldfish.", "content": "In goldfish, injection of ovulated eggs (from donor females) through the ovipore and into the ovarian lumen of females with vitellogenic oocytes induces spawning behaviour within several hours. Intraperitoneal (i.p.) injection of indomethacin (IM), 10 mug/g, either 10 h prior to, or coincident with, injection of ovulated eggs, completely inhibits the onset of spawning behaviour. IM injection similarly terminates ongoing spawning behaviour induced by egg injection. PGF2alpha (5 mug/g; i.p. injection) restores spawning behaviour of egg-injected, IM-blocked fish; at the same dosage, PGE2 is marginally  effective and PGE1 is without effect. As PGF2alpha and PGE2 also induce spawning behaviour in females which have not been injected with ovulated eggs, it is suggested that distension of the oviduct following ovulation or egg injection results in the release of PG which then acts in some way to induce spawning behaviour. The ability of PG to induce spawning behaviour is eliminated by hypophysectomy and restored by treatment with salmon gonadotropin: no steroid treatment was effective in restoring PG-induced spawning in fish which had been hypophysectomized for 3-4 months. The possible mode of action of PG in inducing spawning behaviour in female goldfish is discussed.", "contents": "Effects of indomethacin and prostaglandins on the spawning behaviour of female goldfish. In goldfish, injection of ovulated eggs (from donor females) through the ovipore and into the ovarian lumen of females with vitellogenic oocytes induces spawning behaviour within several hours. Intraperitoneal (i.p.) injection of indomethacin (IM), 10 mug/g, either 10 h prior to, or coincident with, injection of ovulated eggs, completely inhibits the onset of spawning behaviour. IM injection similarly terminates ongoing spawning behaviour induced by egg injection. PGF2alpha (5 mug/g; i.p. injection) restores spawning behaviour of egg-injected, IM-blocked fish; at the same dosage, PGE2 is marginally  effective and PGE1 is without effect. As PGF2alpha and PGE2 also induce spawning behaviour in females which have not been injected with ovulated eggs, it is suggested that distension of the oviduct following ovulation or egg injection results in the release of PG which then acts in some way to induce spawning behaviour. The ability of PG to induce spawning behaviour is eliminated by hypophysectomy and restored by treatment with salmon gonadotropin: no steroid treatment was effective in restoring PG-induced spawning in fish which had been hypophysectomized for 3-4 months. The possible mode of action of PG in inducing spawning behaviour in female goldfish is discussed.", "PMID": 959573}
{"id": "pubmed23n0032_3494", "title": "Prostaglandins and the initiation of labor.", "content": "A total of 96, dated pregnant, New Zealand white rabbits were studied. In 58 animals the intrauterine pressure (IUP) of the unstimulated and PGF2alpha-stimulated myometrium was recorded, by the extraovular microballoon technique, before, during and after parturition. In the remaining 38 the concentrations of PGE and PGF and progesterone (P) were measured by radioimmunoassays (RIA). The samples were collected individually or sequentially during the perinatal period from uterine tissue and uterine or peripheral vein blood. At the critical time, at around parturition, when the myometrium is converted from a suppressed and refractory muscle into a spontaneously active and reactive organ (quantitated by recording the IUP), the uterine PGE and PGF levels decreased rather than increased (quantitated by RIA). Thus, this critical regulatory and functional change of the myometrium cannot be accounted for by an increase in the intrinsic uterine stimulant: PG, but only by a decrease in the suppressor: P. These findings, 46 years after the discovery of P, demand the further exploration of Corner's legacy.", "contents": "Prostaglandins and the initiation of labor. A total of 96, dated pregnant, New Zealand white rabbits were studied. In 58 animals the intrauterine pressure (IUP) of the unstimulated and PGF2alpha-stimulated myometrium was recorded, by the extraovular microballoon technique, before, during and after parturition. In the remaining 38 the concentrations of PGE and PGF and progesterone (P) were measured by radioimmunoassays (RIA). The samples were collected individually or sequentially during the perinatal period from uterine tissue and uterine or peripheral vein blood. At the critical time, at around parturition, when the myometrium is converted from a suppressed and refractory muscle into a spontaneously active and reactive organ (quantitated by recording the IUP), the uterine PGE and PGF levels decreased rather than increased (quantitated by RIA). Thus, this critical regulatory and functional change of the myometrium cannot be accounted for by an increase in the intrinsic uterine stimulant: PG, but only by a decrease in the suppressor: P. These findings, 46 years after the discovery of P, demand the further exploration of Corner's legacy.", "PMID": 959574}
{"id": "pubmed23n0032_3495", "title": "Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2alpha in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry.", "content": "A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2alpha in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2alpha into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13, 14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2alpha found in peripheral plasma are 33 +/- 10 pg/ml (SD; n=6) and 40 +/- 16 pg/ml (SD; n=6), respectively.", "contents": "Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2alpha in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry. A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2alpha in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2alpha into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13, 14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2alpha found in peripheral plasma are 33 +/- 10 pg/ml (SD; n=6) and 40 +/- 16 pg/ml (SD; n=6), respectively.", "PMID": 959575}
{"id": "pubmed23n0032_3496", "title": "Prostaglandins and renal function in acute extracellular volume expansion.", "content": "Mechanisms determining the natriuresis in ECV-expansion are not yet completely understood. The present study was therefore undertaken to investigate if prostaglandins (PG) are involved in the natriuresis of acute ECV-expansion and by which mechanisms PG may affect renal Na-absorption. In non-expanded rats the PG synthetase inhibitor indomethacin (INDO) had no effect on renal function. In 37 Sprague-Dawley rats ECV-expansion with isotonic saline corresponding to an increase in b.wt. of 10% was induced. Twenty-one animals received an oral dose of 10 mg/kg b.wt. of INDO prior to ECV-expansion. Sixteen animals served as ECV-expanded controls (C). GFR (INDO: 12.5 +/- 1.0; C: 10.5 +/- 0.9 ml/min/kg b.wt.) did not significantly differ in both groups. However, total renal plasma flow (RPF) (INDO: 22.9 +/- 1.8; C: 30.1 +/- 2.7 ml/min/kg b.wt.), urinary flow rate (INDO: 1.11 +/- 0.20; C: 1.93 +/- 0.21 ml/min/kg b.wt.) and urinary excretion of sodium (INDO: 141 +/- 26; C: 267 +/- 46 muEq/min/kg b.wt.) and potassium (INDO: 13.0 +/- 0.9; C: 19.8 +/- 1.7 muEq/min/kg b.wt.) markedly decreased in animals pretreated with INDO. The results indicate that PG are involved in the natriuresis of acute ECV-expansion and suggest, that PG may inhibit the intrinsic capacity for Na-absorption in more proximal parts of the nephron possibly via intrarenal physical factors.", "contents": "Prostaglandins and renal function in acute extracellular volume expansion. Mechanisms determining the natriuresis in ECV-expansion are not yet completely understood. The present study was therefore undertaken to investigate if prostaglandins (PG) are involved in the natriuresis of acute ECV-expansion and by which mechanisms PG may affect renal Na-absorption. In non-expanded rats the PG synthetase inhibitor indomethacin (INDO) had no effect on renal function. In 37 Sprague-Dawley rats ECV-expansion with isotonic saline corresponding to an increase in b.wt. of 10% was induced. Twenty-one animals received an oral dose of 10 mg/kg b.wt. of INDO prior to ECV-expansion. Sixteen animals served as ECV-expanded controls (C). GFR (INDO: 12.5 +/- 1.0; C: 10.5 +/- 0.9 ml/min/kg b.wt.) did not significantly differ in both groups. However, total renal plasma flow (RPF) (INDO: 22.9 +/- 1.8; C: 30.1 +/- 2.7 ml/min/kg b.wt.), urinary flow rate (INDO: 1.11 +/- 0.20; C: 1.93 +/- 0.21 ml/min/kg b.wt.) and urinary excretion of sodium (INDO: 141 +/- 26; C: 267 +/- 46 muEq/min/kg b.wt.) and potassium (INDO: 13.0 +/- 0.9; C: 19.8 +/- 1.7 muEq/min/kg b.wt.) markedly decreased in animals pretreated with INDO. The results indicate that PG are involved in the natriuresis of acute ECV-expansion and suggest, that PG may inhibit the intrinsic capacity for Na-absorption in more proximal parts of the nephron possibly via intrarenal physical factors.", "PMID": 959576}
{"id": "pubmed23n0032_3497", "title": "Characterization of prostaglandin production in tissue culture of rat renal medullary cells.", "content": "Renal medullary cells from the rat were used to establish a cell culture line. The morphologic characteristics of these cells were similar to renal medullary interstitial cells. They produced substantial amounts of PGE when provided with arachidonic acid or fetal calf serum. PGE production was inhibited 80-90% by indomethacin or meclofenamate. PGE release by the cells was sensitive to and stimulated by changing the culture media. Stable levels of PGE in the media could be achieved if media changes were avoided during the experimental period.", "contents": "Characterization of prostaglandin production in tissue culture of rat renal medullary cells. Renal medullary cells from the rat were used to establish a cell culture line. The morphologic characteristics of these cells were similar to renal medullary interstitial cells. They produced substantial amounts of PGE when provided with arachidonic acid or fetal calf serum. PGE production was inhibited 80-90% by indomethacin or meclofenamate. PGE release by the cells was sensitive to and stimulated by changing the culture media. Stable levels of PGE in the media could be achieved if media changes were avoided during the experimental period.", "PMID": 959577}
{"id": "pubmed23n0032_3498", "title": "Factors affecting prostaglandin synthesis by rat skin microsomes.", "content": "Prostaglandin synthesis by rat skin was measured by a radiochemical method and the adrenochrome spectrophotometric method. About 70% of the total enzyme activity was located in the microsomal fraction. Incubation with (1-14C) arachidonic acid resulted in the formation of prostaglandins F2alpha, E2 and D2. The biosynthesis of these 3 prostaglandins was stimulated by reduced glutathione and hydroquinone, and was markedly dependent on pH. A number of non-steroid anti-inflammatory drugs produced a dose related inhibition of prostaglandin biosynthesis. Five potent corticosteroid anti-inflammatory agents previously found to be inhibitory when a skin homogenate was used did not inhibit prostaglandin synthesis by the microsomal fraction.", "contents": "Factors affecting prostaglandin synthesis by rat skin microsomes. Prostaglandin synthesis by rat skin was measured by a radiochemical method and the adrenochrome spectrophotometric method. About 70% of the total enzyme activity was located in the microsomal fraction. Incubation with (1-14C) arachidonic acid resulted in the formation of prostaglandins F2alpha, E2 and D2. The biosynthesis of these 3 prostaglandins was stimulated by reduced glutathione and hydroquinone, and was markedly dependent on pH. A number of non-steroid anti-inflammatory drugs produced a dose related inhibition of prostaglandin biosynthesis. Five potent corticosteroid anti-inflammatory agents previously found to be inhibitory when a skin homogenate was used did not inhibit prostaglandin synthesis by the microsomal fraction.", "PMID": 959578}
{"id": "pubmed23n0032_3499", "title": "Effect of prostaglandins on vascular resistance and adrenergic vasoconstrictor responses in the canine uterus.", "content": "Prostaglandins appear to play an important role in a number of reproductive processes. The effect of prostaglandins on numerous vascular beds has been extensively studied, but the effects on uterine hemodynamics have not. The present study was designed to determine the effects of prostaglandins of the A and E series on uterine vascular resistance and responses to adrenergic nerve stimulation in the canine uterus. PGE1, PGE2 and PGA1 were found to be potent uterine vasodilators. In addition, these agents were able to modify adrenergic vasoconstrictor responses. These effects on uterine vascular resistance and adrenergic vasoconstrictor responses were separable. The results of these studies suggest that prostaglandins of the A and E series may play an important role in regulating uterine blood flow in the nonpregnant animal.", "contents": "Effect of prostaglandins on vascular resistance and adrenergic vasoconstrictor responses in the canine uterus. Prostaglandins appear to play an important role in a number of reproductive processes. The effect of prostaglandins on numerous vascular beds has been extensively studied, but the effects on uterine hemodynamics have not. The present study was designed to determine the effects of prostaglandins of the A and E series on uterine vascular resistance and responses to adrenergic nerve stimulation in the canine uterus. PGE1, PGE2 and PGA1 were found to be potent uterine vasodilators. In addition, these agents were able to modify adrenergic vasoconstrictor responses. These effects on uterine vascular resistance and adrenergic vasoconstrictor responses were separable. The results of these studies suggest that prostaglandins of the A and E series may play an important role in regulating uterine blood flow in the nonpregnant animal.", "PMID": 959579}
{"id": "pubmed23n0032_3500", "title": "Rapid block of gonadotropin uptake by corpora lutea in vivo induced by prostaglandin F2alpha.", "content": "Intravenous administration of 125I-hCG to 7-8 day pseudopregnant rats resulted in maximum uptake of radioactivity to corpora lutea 2 hours after treatment. At this time tissue/plasma radioactivity ratios on an equal weight basis were: corpora lutea, 70.2 +/- 12.8; ovarian interstitium, 4.6 +/- 0.2; kidney, 2.2 +/- 0.1. No appreciable uptake was seen by adrenals or liver. Radioactivity in corpora lutea was associated primarily with membranes which sedimented at 2000g and when released by heat it was more than 63% bound to luteal LH receptor preparation in vitro. Radioactivity in renal tissue was associated primarily with the 100,000g supernatant fraction and was bound less than 1% to luteal LH receptors in vitro. PGF2alpha significantly reduced uptake (p less than .001) of 125I-hCG by corpora lutea within 30 minutes (-63%) as well as at 1 (-64%), 2 (-75%), 4 (-68%) and 24 hours (-85%). No clear effect of PGF2alpha on uptake of 125I-hCG by ovarian interstitial tissue was seen. Plasma progesterone was significantly decreased (p less than .001) within 30 minutes (-47%; p less than .01) after PGF2alpha treatment and also at 1 (-65%), 2 (-82%), 4 (-68%) and 24 hours (-92%). Two hours after PGF2alpha treatment the content of progesterone in corpora lutea was depressed (-46%; p less than .001). It is suggested that the rapid inhibition of luteal progesterone production induced by PGF2alpha in vivo occurs through a block in gonadotropin uptake by corpora lutea.", "contents": "Rapid block of gonadotropin uptake by corpora lutea in vivo induced by prostaglandin F2alpha. Intravenous administration of 125I-hCG to 7-8 day pseudopregnant rats resulted in maximum uptake of radioactivity to corpora lutea 2 hours after treatment. At this time tissue/plasma radioactivity ratios on an equal weight basis were: corpora lutea, 70.2 +/- 12.8; ovarian interstitium, 4.6 +/- 0.2; kidney, 2.2 +/- 0.1. No appreciable uptake was seen by adrenals or liver. Radioactivity in corpora lutea was associated primarily with membranes which sedimented at 2000g and when released by heat it was more than 63% bound to luteal LH receptor preparation in vitro. Radioactivity in renal tissue was associated primarily with the 100,000g supernatant fraction and was bound less than 1% to luteal LH receptors in vitro. PGF2alpha significantly reduced uptake (p less than .001) of 125I-hCG by corpora lutea within 30 minutes (-63%) as well as at 1 (-64%), 2 (-75%), 4 (-68%) and 24 hours (-85%). No clear effect of PGF2alpha on uptake of 125I-hCG by ovarian interstitial tissue was seen. Plasma progesterone was significantly decreased (p less than .001) within 30 minutes (-47%; p less than .01) after PGF2alpha treatment and also at 1 (-65%), 2 (-82%), 4 (-68%) and 24 hours (-92%). Two hours after PGF2alpha treatment the content of progesterone in corpora lutea was depressed (-46%; p less than .001). It is suggested that the rapid inhibition of luteal progesterone production induced by PGF2alpha in vivo occurs through a block in gonadotropin uptake by corpora lutea.", "PMID": 959580}
{"id": "pubmed23n0032_3501", "title": "Effects of prostaglandins on leukocyte migration.", "content": "Artificially synthesized prostaglandins (PGE1, PGE2, PGF1alpha, and PGF2alpha) were found, using Boyden's chamber, to induce significant migration of polymorphonuclear leukocytes (PMNs) of the rabbit; PGF2alpha had greater effects than PGE1 or E2. A typical dose dependent relationship was found between the PMNs migration and PGF2alpha concentrations. Indomethacin pretreatments of rabbits did not significantly alter the PMNs migration indicating that PGs synthetized in vivo was not involved in the migration. PGF2alpha was placed in the lower compartment opposite to PMNs and also in the upper compartment together with PMNs. No significant difference was found in the number of migrated PMNs between the two experimental conditions. PGs diffusion occurred across the millipore filter separating the two compartments where the concentrations were almost equal at the end of 3 hours incubation. It was thus concluded that PGs effects are to induce random PMNs movements rather than to initiate chemotactic directional migration.", "contents": "Effects of prostaglandins on leukocyte migration. Artificially synthesized prostaglandins (PGE1, PGE2, PGF1alpha, and PGF2alpha) were found, using Boyden's chamber, to induce significant migration of polymorphonuclear leukocytes (PMNs) of the rabbit; PGF2alpha had greater effects than PGE1 or E2. A typical dose dependent relationship was found between the PMNs migration and PGF2alpha concentrations. Indomethacin pretreatments of rabbits did not significantly alter the PMNs migration indicating that PGs synthetized in vivo was not involved in the migration. PGF2alpha was placed in the lower compartment opposite to PMNs and also in the upper compartment together with PMNs. No significant difference was found in the number of migrated PMNs between the two experimental conditions. PGs diffusion occurred across the millipore filter separating the two compartments where the concentrations were almost equal at the end of 3 hours incubation. It was thus concluded that PGs effects are to induce random PMNs movements rather than to initiate chemotactic directional migration.", "PMID": 959581}
{"id": "pubmed23n0032_3502", "title": "Indomethacin potentiates the vasoconstrictor actions of angiotensin II in normal man.", "content": "The blood pressure response to graded infusions of angiotensin II was assessed under control conditions and following short term (16 hour) indomethacin treatment utilizing normal men equilibrated on a constant diet of normal sodium and potassium content. Although basal mean blood pressure was unchanged, the increase in blood pressure with all rates of angiotensin II infusion ranging from 200 to 1000 ng/min was significantly greater with indomethacin treatment. Pre-infusion body weight and plasma renin activity were similar under the two conditions. These results suggest that prostaglandins modulate the systemic vasoconstrictor effects of angiotensin II.", "contents": "Indomethacin potentiates the vasoconstrictor actions of angiotensin II in normal man. The blood pressure response to graded infusions of angiotensin II was assessed under control conditions and following short term (16 hour) indomethacin treatment utilizing normal men equilibrated on a constant diet of normal sodium and potassium content. Although basal mean blood pressure was unchanged, the increase in blood pressure with all rates of angiotensin II infusion ranging from 200 to 1000 ng/min was significantly greater with indomethacin treatment. Pre-infusion body weight and plasma renin activity were similar under the two conditions. These results suggest that prostaglandins modulate the systemic vasoconstrictor effects of angiotensin II.", "PMID": 959582}
{"id": "pubmed23n0032_3503", "title": "The levels of main urinary metabolite of prostaglandin F1alpha and F2alpha in human subjects measured by radioimmunoassay.", "content": "Radioimmunoassay technique for measuring 5alpha, 7alpha-dihydroxy-11-keto-tetranorprosta-1, 16-dioic acid, the main urinary metabolite of PGF1alpha and PGF2alpha (PGF2alpha-MUM), was further improved. It was postulated based on some experimental data that the PGF2alpha-MUM exists in the urine mostly as dioic acid form, not as delta-lactone form the daily excretion of PGF2alpha-MUM in men ranged from 14.43 mug to 36.14 mug and in women from 5.21 mug to 14.25 mug.", "contents": "The levels of main urinary metabolite of prostaglandin F1alpha and F2alpha in human subjects measured by radioimmunoassay. Radioimmunoassay technique for measuring 5alpha, 7alpha-dihydroxy-11-keto-tetranorprosta-1, 16-dioic acid, the main urinary metabolite of PGF1alpha and PGF2alpha (PGF2alpha-MUM), was further improved. It was postulated based on some experimental data that the PGF2alpha-MUM exists in the urine mostly as dioic acid form, not as delta-lactone form the daily excretion of PGF2alpha-MUM in men ranged from 14.43 mug to 36.14 mug and in women from 5.21 mug to 14.25 mug.", "PMID": 959583}
{"id": "pubmed23n0032_3504", "title": "Inhibition of prostaglandin biosynthesis by triynoic acids.", "content": "Prostaglandin biosynthesis from eicosa-8,11,14-trienoic acid in microsomes from bovine seminal vesicles is inhibited by acetylenic acids. Octadeca-6,9,12-triynoic acid and eicosa-8,11,14-triynoic acid are the most potent inhibitors. These acids both contain an omega-8 methylene group. Within the 20-carbon acetylenic acid series, inhibition decreases in the sequence eicosa-8,11,14-triynoic acid greater than eicosa-7,10,13-triynoic acid greater than eicosa-5,8,11-triynoic acid. Furthermore, eicosa-8,11,14-triynoic acid is a more potent inhibitor of arachidonic acid induced platelet aggregation than either eicosa-7,10,13-triynoic acid or eicosa-5,8,11-triynoic acid. The omega-8 methylene group is not the only determinent of inhibitory potency since docosa-10,13,16-triynoic acid is less potent than its 18 and 20 carbon analogs and all of these acids have an omega-8 methylene group.", "contents": "Inhibition of prostaglandin biosynthesis by triynoic acids. Prostaglandin biosynthesis from eicosa-8,11,14-trienoic acid in microsomes from bovine seminal vesicles is inhibited by acetylenic acids. Octadeca-6,9,12-triynoic acid and eicosa-8,11,14-triynoic acid are the most potent inhibitors. These acids both contain an omega-8 methylene group. Within the 20-carbon acetylenic acid series, inhibition decreases in the sequence eicosa-8,11,14-triynoic acid greater than eicosa-7,10,13-triynoic acid greater than eicosa-5,8,11-triynoic acid. Furthermore, eicosa-8,11,14-triynoic acid is a more potent inhibitor of arachidonic acid induced platelet aggregation than either eicosa-7,10,13-triynoic acid or eicosa-5,8,11-triynoic acid. The omega-8 methylene group is not the only determinent of inhibitory potency since docosa-10,13,16-triynoic acid is less potent than its 18 and 20 carbon analogs and all of these acids have an omega-8 methylene group.", "PMID": 959584}
{"id": "pubmed23n0032_3505", "title": "Inhibition of renal PGE2-9-ketoreductase by diuretics.", "content": "The metabolism of PGE2 by extracts of renal cortex is species dependent. In the rat PGE2-15-hydroxydehydrogenase initiates metabolism whereas in the rabbit PGE2-9-ketoreductase predominates. In man both mechanisms may operate. Each of the metabolic enzymes, which limits the vasodilator-diuretic actions of PGE2, was inhibited by ethacrynic acid, furosemide and indomethacin. Some inhibition of PGE2-9-ketoreductase was also observed with chlorthalidone, hydralazine and phentolamine but the thiazide diuretics and a number of other cardiovascular-active agents were without significant effect. We conclude that the inhibition of PGE2-9-ketoreductase and PGE2-15-hydroxydehydrogeanse could contribute to the mechanism of action of the non-thiaxide diuretics in man.", "contents": "Inhibition of renal PGE2-9-ketoreductase by diuretics. The metabolism of PGE2 by extracts of renal cortex is species dependent. In the rat PGE2-15-hydroxydehydrogenase initiates metabolism whereas in the rabbit PGE2-9-ketoreductase predominates. In man both mechanisms may operate. Each of the metabolic enzymes, which limits the vasodilator-diuretic actions of PGE2, was inhibited by ethacrynic acid, furosemide and indomethacin. Some inhibition of PGE2-9-ketoreductase was also observed with chlorthalidone, hydralazine and phentolamine but the thiazide diuretics and a number of other cardiovascular-active agents were without significant effect. We conclude that the inhibition of PGE2-9-ketoreductase and PGE2-15-hydroxydehydrogeanse could contribute to the mechanism of action of the non-thiaxide diuretics in man.", "PMID": 959586}
{"id": "pubmed23n0032_3506", "title": "The role of protein synthesis in the stimulation by LH of prostaglandin accumulation in rat preovulatory follicles in vitro.", "content": "Preovulatory follicles isolated from immature rats, treated in vivo with pregnant mare's serum gonadotropin, were incubated in vitro and the accumulation of prostaglandin E measured. The addition of luteinizing hormone (5 mug/ml) increased this accumulation, after a lag period of 3 hours. This delay suggested the involvement of macromolecular synthesis in the mechanism of prostaglandin stimulation by luteinizing hormone. When the synthesis of protein was inhibited by the addition of puromycin (100 muM), the luteinizing hormone stimulation of prostaglandin E in these follicles was completely abolished. This inhibition was not seen with an analogue of puromycin, which does not inhibit protein synthesis, puromycin amino-nucleoside. These data suggest that concomitant protein synthesis is required for the luteinzing hormone stimulation of prostaglandin accumulation in rat follicles.", "contents": "The role of protein synthesis in the stimulation by LH of prostaglandin accumulation in rat preovulatory follicles in vitro. Preovulatory follicles isolated from immature rats, treated in vivo with pregnant mare's serum gonadotropin, were incubated in vitro and the accumulation of prostaglandin E measured. The addition of luteinizing hormone (5 mug/ml) increased this accumulation, after a lag period of 3 hours. This delay suggested the involvement of macromolecular synthesis in the mechanism of prostaglandin stimulation by luteinizing hormone. When the synthesis of protein was inhibited by the addition of puromycin (100 muM), the luteinizing hormone stimulation of prostaglandin E in these follicles was completely abolished. This inhibition was not seen with an analogue of puromycin, which does not inhibit protein synthesis, puromycin amino-nucleoside. These data suggest that concomitant protein synthesis is required for the luteinzing hormone stimulation of prostaglandin accumulation in rat follicles.", "PMID": 959587}
{"id": "pubmed23n0032_3507", "title": "Avian oviduct motility induced by prostaglandin E1.", "content": "Oviduct segments from infundibulum, magnum, uterus, utero-vaginal junction and vagina of actively laying hens at preoviposition time were tested for their contractile reaction to prostaglandin E1 by in vitro or in vivo methods. Maximum stimulatory response was observed from the muscular strips of the proximal oviduct segment (infundibulum) and a complete relaxation was recorded from the distal part (vagina) at molar concentrations of 1.4 X 10(-7), 3.4 X 10(-7) and 7.0 X 10(-7). The uterine strips reacted with a stimulatory response at higher concentrations (1.4 X 10(-6) and 2.8 X 10(-6) moles), but lacked any significant change at lower concentrations. The uterovaginal muscular strips showed a mild but prolonged inhibitory response, while the magnum responded with a significant increase in the luminal pressure when tested in vivo. It is concluded that PGE1 exerts a stimulatory effect on the uterus to initiate transport of the egg to subsequent segments (utero-vaginal junction and vagina), which relax under PGE1 influence and allow passage of the egg by pressure differences.", "contents": "Avian oviduct motility induced by prostaglandin E1. Oviduct segments from infundibulum, magnum, uterus, utero-vaginal junction and vagina of actively laying hens at preoviposition time were tested for their contractile reaction to prostaglandin E1 by in vitro or in vivo methods. Maximum stimulatory response was observed from the muscular strips of the proximal oviduct segment (infundibulum) and a complete relaxation was recorded from the distal part (vagina) at molar concentrations of 1.4 X 10(-7), 3.4 X 10(-7) and 7.0 X 10(-7). The uterine strips reacted with a stimulatory response at higher concentrations (1.4 X 10(-6) and 2.8 X 10(-6) moles), but lacked any significant change at lower concentrations. The uterovaginal muscular strips showed a mild but prolonged inhibitory response, while the magnum responded with a significant increase in the luminal pressure when tested in vivo. It is concluded that PGE1 exerts a stimulatory effect on the uterus to initiate transport of the egg to subsequent segments (utero-vaginal junction and vagina), which relax under PGE1 influence and allow passage of the egg by pressure differences.", "PMID": 959588}
{"id": "pubmed23n0032_3508", "title": "Considerations into the mechanism of estrogen-stimulated uterine prostaglandin synthesis.", "content": "Progesterone priming of the ovariectomized rat, followed by a single injection of estradiol-17beta (10 mug) is followed by an increased uterine synthesis of both PGF and PGE. The administration of an estrogen antagonist (MER-25; 10 mg) concomitantly with estradiol had no effect on uterine prostaglandin synthesis. Similarly, the administration of either Actinomycin D or cycloheximide, antibiotics demonstrated to inhibit mRNA and protein synthesis, respectively, is without effect on estrogen-stimulated uterine prostaglandin synthesis. These results are considered with regard to the classic receptor theory of estrogen action and are a preliminary indication that estrogen-stimulated uterine prostaglandin synthesis may not require those receptor mediated events.", "contents": "Considerations into the mechanism of estrogen-stimulated uterine prostaglandin synthesis. Progesterone priming of the ovariectomized rat, followed by a single injection of estradiol-17beta (10 mug) is followed by an increased uterine synthesis of both PGF and PGE. The administration of an estrogen antagonist (MER-25; 10 mg) concomitantly with estradiol had no effect on uterine prostaglandin synthesis. Similarly, the administration of either Actinomycin D or cycloheximide, antibiotics demonstrated to inhibit mRNA and protein synthesis, respectively, is without effect on estrogen-stimulated uterine prostaglandin synthesis. These results are considered with regard to the classic receptor theory of estrogen action and are a preliminary indication that estrogen-stimulated uterine prostaglandin synthesis may not require those receptor mediated events.", "PMID": 959589}
{"id": "pubmed23n0032_3509", "title": "The effects of progesterone, prostaglandin F2alpha and oxytocin on the calcium-activation of the uterus.", "content": "The relationship between \"activator-calcium\" (A-Ca), progesterone (P), prostaglandin F2alpha (PGF2alpha) and oxytocin (Oxy) has been examined in 100 uterine strips of 34 pregnant and 100 strips of 34 post partum rabbits. At the 25th day of gestation, uterine P was 13.9+/-1.3 ng/g, while within 3-12 hours post partum 3.3+/-0.3 ng/g tissue (P less than 0.001). Uterine strips, mounted isometrically in Krebs' solution, sustained maximum excitability in a steady state when exposed every 30 seconds for 4 seconds to an electric field of 12 V/5 cm (a.c.). The maximally contracting muscles were then rinsed at intervals of 6 minutes with Ca-free Krebs. In Ca-free Krebs, the post partum uterus lost 31% of its Ca and 96% of its excitability in a short 25 minutes, while the pregnant uterus lost 30% of its Ca and 93% of its excitability in 50 minutes (P less than 0.001). Since the extracellular space is approximately 30% in the uterus, this approximately 30% Ca, lost by both muscles, most probably was extracellular Ca and the small A-Ca fraction which is presumably \"bound\" more strongly at the membrane systems of the P-dominated pregnant, than the non-dominated post partum uterus. The significantly faster and more complete recovery from Ca-deficiency and inexcitability of the pregnant than the post partum uterus (P less than 0.001), at different levels of external Ca, further substantiates this premise. So does the demonstration that exposure to Ca-free Krebs increases 45Ca-efflux 400% in the post partum and only 110% in the pregnant uterus (P less than 0.001). Exposure to 100 ng/ml PGF2alpha in normal Krebs has a similar effect on the 45Ca-efflux of the post partum uterus, while the response of the pregnant uterus is indistinct (P less than 0.001). These highly significant differences between the post partum and the pregnant uteri in their Ca-efflux explain the higher threshold (P less than 0.001) and lower \"sensitivity\" to PGF2alpha and Oxy (P less than 0.001) of the pregnant than the post partum uterus. The already very highly significant differences between the two muscles, in threshold and sensitivity to these two most potent oxytocics, were increased still further by rendering the uterine strips Ca-deficient. All together, these findings substantiate the early contention (1-7, 18, 19) that uterine function at the cellular level is regulated by opposing actions of the suppressor P and the intrinsic stimulant PG or other oxytocic agents on threshold, excitability and the Ca-activation of the contractile process.", "contents": "The effects of progesterone, prostaglandin F2alpha and oxytocin on the calcium-activation of the uterus. The relationship between \"activator-calcium\" (A-Ca), progesterone (P), prostaglandin F2alpha (PGF2alpha) and oxytocin (Oxy) has been examined in 100 uterine strips of 34 pregnant and 100 strips of 34 post partum rabbits. At the 25th day of gestation, uterine P was 13.9+/-1.3 ng/g, while within 3-12 hours post partum 3.3+/-0.3 ng/g tissue (P less than 0.001). Uterine strips, mounted isometrically in Krebs' solution, sustained maximum excitability in a steady state when exposed every 30 seconds for 4 seconds to an electric field of 12 V/5 cm (a.c.). The maximally contracting muscles were then rinsed at intervals of 6 minutes with Ca-free Krebs. In Ca-free Krebs, the post partum uterus lost 31% of its Ca and 96% of its excitability in a short 25 minutes, while the pregnant uterus lost 30% of its Ca and 93% of its excitability in 50 minutes (P less than 0.001). Since the extracellular space is approximately 30% in the uterus, this approximately 30% Ca, lost by both muscles, most probably was extracellular Ca and the small A-Ca fraction which is presumably \"bound\" more strongly at the membrane systems of the P-dominated pregnant, than the non-dominated post partum uterus. The significantly faster and more complete recovery from Ca-deficiency and inexcitability of the pregnant than the post partum uterus (P less than 0.001), at different levels of external Ca, further substantiates this premise. So does the demonstration that exposure to Ca-free Krebs increases 45Ca-efflux 400% in the post partum and only 110% in the pregnant uterus (P less than 0.001). Exposure to 100 ng/ml PGF2alpha in normal Krebs has a similar effect on the 45Ca-efflux of the post partum uterus, while the response of the pregnant uterus is indistinct (P less than 0.001). These highly significant differences between the post partum and the pregnant uteri in their Ca-efflux explain the higher threshold (P less than 0.001) and lower \"sensitivity\" to PGF2alpha and Oxy (P less than 0.001) of the pregnant than the post partum uterus. The already very highly significant differences between the two muscles, in threshold and sensitivity to these two most potent oxytocics, were increased still further by rendering the uterine strips Ca-deficient. All together, these findings substantiate the early contention (1-7, 18, 19) that uterine function at the cellular level is regulated by opposing actions of the suppressor P and the intrinsic stimulant PG or other oxytocic agents on threshold, excitability and the Ca-activation of the contractile process.", "PMID": 959590}
{"id": "pubmed23n0032_3510", "title": "Further studies on prostaglandin E2 (PGE2)-induced gonadotropin release: comparison with the effect of 15-methyl PGE2, PGAs, PGBs and prostaglandin endoperoxide analogs.", "content": "It is known that PGE2 is a potent stimulus of LH release. To determine if the effect of PGE2 could be enhanced and/or prolonged by retarding its metabolic degradation, a derivative, 15-methyl PGE2 (15-E2) which is more slowly degraded than the natural compound was injected intravenously (i.v.) at various dose levels or into the third ventricle (3rd V) of ether-anesthetized, ovariectomized, estrogen (OVX, Eb)-treated rats and its effect on gonadotropin release was compared with that of PGE2. Both PGs injected i.v. were equally effective in increasing plamsa LH and maintaining the elevated levels, although 15-E2 induced a larger and more sustained increase in plasma FSH than PGE2. By contrast, 3rd V PGE2 was clearly more effective than 3rd V 15-E2 in releasing LH and to a lesser extent, FSH. The effect of 15-E2 on LH was similar to that produced by 3rd V PGE1, injected at a similar dose. However, its effect on FSH was greater than that of PGE1. To evaluate the effect(s) of prostaglandins of the A and B series on gonadotropin release, PGA1, PGA2, PGB1 or PGB2 were injected intraventricularly in OVX, Eb-treated rats. PGBs were injected into conscious, free-moving rats. PGA2 or PGB2 increased plasma LH concentrations although much less effectively than PGE2. Third V PGA1 or PGB1 were ineffective. The 3rd V injection of two cyclic esters (U-44069 and U-46619), stable analogs of the PG endoperoxide PGG2 and PGH2, induced a small, transient increase in LH levels and did not alter plasma FSH in conscious, free-moving animals. PGE2 injected intraventricularly at a similar dose was demonstrated to be mcuh more potent than the analogs in stimulating LH and FSH release. The results indicate that: 1) 15-E2, in spite of its described long-lasting activity, does not appear to be more potent than the natural compound in releasing LH, although when injected i.v., it appeared to induce a more sustained increase in plasma FSH; 2) although PGA2 and PGB2 can also act centrally to stimulate LH release, their low potency suggests that this is a pharmacological effect; and 3) the two analogs of PG endoperoxides tested proved to be poor stimuli for gonadotropin release. The significance of these findings is discussed.", "contents": "Further studies on prostaglandin E2 (PGE2)-induced gonadotropin release: comparison with the effect of 15-methyl PGE2, PGAs, PGBs and prostaglandin endoperoxide analogs. It is known that PGE2 is a potent stimulus of LH release. To determine if the effect of PGE2 could be enhanced and/or prolonged by retarding its metabolic degradation, a derivative, 15-methyl PGE2 (15-E2) which is more slowly degraded than the natural compound was injected intravenously (i.v.) at various dose levels or into the third ventricle (3rd V) of ether-anesthetized, ovariectomized, estrogen (OVX, Eb)-treated rats and its effect on gonadotropin release was compared with that of PGE2. Both PGs injected i.v. were equally effective in increasing plamsa LH and maintaining the elevated levels, although 15-E2 induced a larger and more sustained increase in plasma FSH than PGE2. By contrast, 3rd V PGE2 was clearly more effective than 3rd V 15-E2 in releasing LH and to a lesser extent, FSH. The effect of 15-E2 on LH was similar to that produced by 3rd V PGE1, injected at a similar dose. However, its effect on FSH was greater than that of PGE1. To evaluate the effect(s) of prostaglandins of the A and B series on gonadotropin release, PGA1, PGA2, PGB1 or PGB2 were injected intraventricularly in OVX, Eb-treated rats. PGBs were injected into conscious, free-moving rats. PGA2 or PGB2 increased plasma LH concentrations although much less effectively than PGE2. Third V PGA1 or PGB1 were ineffective. The 3rd V injection of two cyclic esters (U-44069 and U-46619), stable analogs of the PG endoperoxide PGG2 and PGH2, induced a small, transient increase in LH levels and did not alter plasma FSH in conscious, free-moving animals. PGE2 injected intraventricularly at a similar dose was demonstrated to be mcuh more potent than the analogs in stimulating LH and FSH release. The results indicate that: 1) 15-E2, in spite of its described long-lasting activity, does not appear to be more potent than the natural compound in releasing LH, although when injected i.v., it appeared to induce a more sustained increase in plasma FSH; 2) although PGA2 and PGB2 can also act centrally to stimulate LH release, their low potency suggests that this is a pharmacological effect; and 3) the two analogs of PG endoperoxides tested proved to be poor stimuli for gonadotropin release. The significance of these findings is discussed.", "PMID": 959591}
{"id": "pubmed23n0032_3511", "title": "Electroconvulsive shock and norepinephrine uptake kinetics in the rat brain.", "content": "The kinetic constants, apparent Km and Vmax, for the uptake of 3H-1-norepinephrine in cerebral cortical homogenates of the rat brain were not altered 5 min after a single electroconvulsive shock (ECS). However, one day after the last of a series of 14 ECS the apparent Km and Vmax were significantly increased above sham-shock controls, and ECS rats lost weight. Three days after the last ECS apparent Km and Vmax were significantly decreased, while the rate of weight gain tripled in the ECS rats. One week after the last ECS the kinetic constants and rate of weight gain were no different from sham-shock controls. These data are indicative of the strong compensatory mechanisms in the normal rat to the alterations induced by ECS.", "contents": "Electroconvulsive shock and norepinephrine uptake kinetics in the rat brain. The kinetic constants, apparent Km and Vmax, for the uptake of 3H-1-norepinephrine in cerebral cortical homogenates of the rat brain were not altered 5 min after a single electroconvulsive shock (ECS). However, one day after the last of a series of 14 ECS the apparent Km and Vmax were significantly increased above sham-shock controls, and ECS rats lost weight. Three days after the last ECS apparent Km and Vmax were significantly decreased, while the rate of weight gain tripled in the ECS rats. One week after the last ECS the kinetic constants and rate of weight gain were no different from sham-shock controls. These data are indicative of the strong compensatory mechanisms in the normal rat to the alterations induced by ECS.", "PMID": 959601}
{"id": "pubmed23n0032_3512", "title": "The contribution of epidemiology to knowledge of cancer.", "content": "Epidemiology has contributed to knowledge of cancer in five ways. First, it has demonstrated that all cancers that are at all common anywhere vary in incidence from place to place and from time to time. The amount of variation is large, sometimes several hundred fold, and no cancers are so uniformly common that they would affect as many as 1 per cent of people by 75 years of age, in the absence of other causes of death, in all countries. Studies of migrant groups and the changes that have occurred with time show that this variation is, in large part, due to environmental factors and that most cancers are, in principle, preventible. Secondly it has shown that the incidence of specific cancers can be correlated with the prevalence of factors in the environment that might be suspected of causing the disease. Recent correlations include those between oesophageal cancer in Iran and the consumption of certain locally produced foods, between colon cancer and the presence of certain groups of anaerobic bacteria in the faeces, and between liver cancer and the amount of aflatoxin in the diet. These correlations do not provide direct evidence of causation, but they do suggest ideas for investigation by other methods. Thirdly, epidemiology has provided means for testing hypotheses by relating the occurrence of particular types of cancer to the personal characteristics of individuals, either by studying the past experience of people with and without cancer, or by following up people whose exposure to suspected agents has been previously defined. In this way it has been possible to detect dozens of occupational hazards in industry, which is many more than have been detected by laboratory experiment. Fourthly, epidemiology has confirmed that an agent is a cause of cancer by monitoring the effects of intervention to reduce or prevent exposure. An example is provided by observation in British doctors, who have reduced their consumption of cigarettes to half the national average and have experienced a corresponding reduction in the risk of death from cancers of the lung.", "contents": "The contribution of epidemiology to knowledge of cancer. Epidemiology has contributed to knowledge of cancer in five ways. First, it has demonstrated that all cancers that are at all common anywhere vary in incidence from place to place and from time to time. The amount of variation is large, sometimes several hundred fold, and no cancers are so uniformly common that they would affect as many as 1 per cent of people by 75 years of age, in the absence of other causes of death, in all countries. Studies of migrant groups and the changes that have occurred with time show that this variation is, in large part, due to environmental factors and that most cancers are, in principle, preventible. Secondly it has shown that the incidence of specific cancers can be correlated with the prevalence of factors in the environment that might be suspected of causing the disease. Recent correlations include those between oesophageal cancer in Iran and the consumption of certain locally produced foods, between colon cancer and the presence of certain groups of anaerobic bacteria in the faeces, and between liver cancer and the amount of aflatoxin in the diet. These correlations do not provide direct evidence of causation, but they do suggest ideas for investigation by other methods. Thirdly, epidemiology has provided means for testing hypotheses by relating the occurrence of particular types of cancer to the personal characteristics of individuals, either by studying the past experience of people with and without cancer, or by following up people whose exposure to suspected agents has been previously defined. In this way it has been possible to detect dozens of occupational hazards in industry, which is many more than have been detected by laboratory experiment. Fourthly, epidemiology has confirmed that an agent is a cause of cancer by monitoring the effects of intervention to reduce or prevent exposure. An example is provided by observation in British doctors, who have reduced their consumption of cigarettes to half the national average and have experienced a corresponding reduction in the risk of death from cancers of the lung.", "PMID": 959627}
{"id": "pubmed23n0032_3513", "title": "[Estimation of risk as a function of risk factors. II.--Application to the multivariate analysis of coronary heart disease in a middle aged male population (author's transl)].", "content": "The annual incidence of coronary heart disease (CHD) in a 8000 male professionnal group, aged 42-53, is computed from the model of LELLOUCH and al. described in the first part of this work. It is shown that each of five risk factors (cholesterol, blood pressure, cigarettes consumption, diabetes history and abnormal electrocardiogram) brings its own contribution to the CHD risk, which may be estimated by an exponential function of a linear combination of the levels of the five factors. \"Risk tables\" useful in current practice are described.", "contents": "[Estimation of risk as a function of risk factors. II.--Application to the multivariate analysis of coronary heart disease in a middle aged male population (author's transl)]. The annual incidence of coronary heart disease (CHD) in a 8000 male professionnal group, aged 42-53, is computed from the model of LELLOUCH and al. described in the first part of this work. It is shown that each of five risk factors (cholesterol, blood pressure, cigarettes consumption, diabetes history and abnormal electrocardiogram) brings its own contribution to the CHD risk, which may be estimated by an exponential function of a linear combination of the levels of the five factors. \"Risk tables\" useful in current practice are described.", "PMID": 959629}
{"id": "pubmed23n0032_3514", "title": "[Epidemiology of S. aureus cross-infections in dermatological wards].", "content": "Over a three-year period, 196 of 3115 patients admitted in a dermatological department became infected with S. aureus (6,2 %). 205 strains of S. aureus were isolated. Serologic typing, phage-typing and antibiotic sensitivity tests revealed 3 epidemic and 2 endemic strains. The 3 epidemic strains infected 24 patients: 12 from july to november 1972 were infected with a serotype 66438 S. aureus resistant to fusidic acid. 6 patients (male) were infected with a serotype III in february and march 1972; 8 patients were contaminated with a serotype 18 S. aureus from december 1973 to february 1974, after staying in a surgical department. Of the 2 endemic strains 1, phage-pattern 53/79, is non-typable by serologic-typing; this strain has been observed only in the dermatological department and 20 patients were infected with, from january to october 1974. The second endemic strain, phage-pattern 81/+ serotype I, cross-infected 16 patients during this three-year survey; 12 of them were admitted repeatedly. During this three-year survey, it could be proved that, at least, 1 out of 3 patients is infected with an epidemic or an endemic strain. We can suggest that the factors enhancing cross-infection in dermatological department are: the sex of patients (80 % were male); presence of a tween splitting enzyme by S. aureus promotes growth of Staphylococci on the skin; patients transfered from a department to another or repeatedly admitted are more often infected. But, as they are source of some outbreaks, they need special measures (asepsis and hygiene); cortico\u00efds or immunodepressors enhance cross-infection; antibiotics must not be only limited but varied too.", "contents": "[Epidemiology of S. aureus cross-infections in dermatological wards]. Over a three-year period, 196 of 3115 patients admitted in a dermatological department became infected with S. aureus (6,2 %). 205 strains of S. aureus were isolated. Serologic typing, phage-typing and antibiotic sensitivity tests revealed 3 epidemic and 2 endemic strains. The 3 epidemic strains infected 24 patients: 12 from july to november 1972 were infected with a serotype 66438 S. aureus resistant to fusidic acid. 6 patients (male) were infected with a serotype III in february and march 1972; 8 patients were contaminated with a serotype 18 S. aureus from december 1973 to february 1974, after staying in a surgical department. Of the 2 endemic strains 1, phage-pattern 53/79, is non-typable by serologic-typing; this strain has been observed only in the dermatological department and 20 patients were infected with, from january to october 1974. The second endemic strain, phage-pattern 81/+ serotype I, cross-infected 16 patients during this three-year survey; 12 of them were admitted repeatedly. During this three-year survey, it could be proved that, at least, 1 out of 3 patients is infected with an epidemic or an endemic strain. We can suggest that the factors enhancing cross-infection in dermatological department are: the sex of patients (80 % were male); presence of a tween splitting enzyme by S. aureus promotes growth of Staphylococci on the skin; patients transfered from a department to another or repeatedly admitted are more often infected. But, as they are source of some outbreaks, they need special measures (asepsis and hygiene); cortico\u00efds or immunodepressors enhance cross-infection; antibiotics must not be only limited but varied too.", "PMID": 959631}
{"id": "pubmed23n0032_3515", "title": "[Estimation of risk as a function of risk factors: 1. Description of the method (author's transl)].", "content": "This paper describes a model which makes allowance for unequal follow up times in estimating the risk as a function of risk factors. It is the exponential model with a hazard which is also an exponential function of risk factors. Computing methods and an extension of the model are given.", "contents": "[Estimation of risk as a function of risk factors: 1. Description of the method (author's transl)]. This paper describes a model which makes allowance for unequal follow up times in estimating the risk as a function of risk factors. It is the exponential model with a hazard which is also an exponential function of risk factors. Computing methods and an extension of the model are given.", "PMID": 959628}
{"id": "pubmed23n0032_3516", "title": "[Chronic bronchitis and risk factors in employees of the sanitation d\u00e9partment, city of Geneva].", "content": "The MRC questionnaire on respiratory symptoms was applied to 429 employees of the sanitation department of the city of Geneva. At the same time, the PFR was determined. The prevalence of the chronic bronchitis was shown to be three times as high in this sample as in the active population of Geneva from the same age group. According to our results, the severity of the respiratory symptoms as well as the level of the respiratory function depended, besides age, on the three risk factors which had been taken into account: cigarette smoking, a history of broncho-pulmonary disease and occupational exposure to weather conditions and air pollution. In the absence of these factors, the severity of symptoms was the lowest and the respiratory function the highest. With all these factors combined, the severity of symptoms was the most pronounced and the function the worst, particularly in bronchitics. It seems that prevention of chronic bronchitis should not rely only upon antismoking propaganda but that the combination of several risks factors should be avoided.", "contents": "[Chronic bronchitis and risk factors in employees of the sanitation d\u00e9partment, city of Geneva]. The MRC questionnaire on respiratory symptoms was applied to 429 employees of the sanitation department of the city of Geneva. At the same time, the PFR was determined. The prevalence of the chronic bronchitis was shown to be three times as high in this sample as in the active population of Geneva from the same age group. According to our results, the severity of the respiratory symptoms as well as the level of the respiratory function depended, besides age, on the three risk factors which had been taken into account: cigarette smoking, a history of broncho-pulmonary disease and occupational exposure to weather conditions and air pollution. In the absence of these factors, the severity of symptoms was the lowest and the respiratory function the highest. With all these factors combined, the severity of symptoms was the most pronounced and the function the worst, particularly in bronchitics. It seems that prevention of chronic bronchitis should not rely only upon antismoking propaganda but that the combination of several risks factors should be avoided.", "PMID": 959630}
{"id": "pubmed23n0032_3517", "title": "[On the vocational prognoses of patients with peroneal muscular atrophy (author's transl)].", "content": "Examined were 43 patients and 27 relatives all suffering from a hypertrophic type of peroneal muscular atrophy. Owing to foot deformities and a slowing of the nerve conduction velocity a diagnosis was already possible in their childhoods in the majority of cases. Although, after the 20th year of age, the pareses of the legs progress only slowly, considerable pareses of the small hand muscles can still develop in adults. With adequate counselling, rehabilitation measures should become necessary only in rare cases, or, if a kypho-scoliosis has already developed in childhood (10% of our cases).", "contents": "[On the vocational prognoses of patients with peroneal muscular atrophy (author's transl)]. Examined were 43 patients and 27 relatives all suffering from a hypertrophic type of peroneal muscular atrophy. Owing to foot deformities and a slowing of the nerve conduction velocity a diagnosis was already possible in their childhoods in the majority of cases. Although, after the 20th year of age, the pareses of the legs progress only slowly, considerable pareses of the small hand muscles can still develop in adults. With adequate counselling, rehabilitation measures should become necessary only in rare cases, or, if a kypho-scoliosis has already developed in childhood (10% of our cases).", "PMID": 959640}
{"id": "pubmed23n0032_3518", "title": "[Trampoline therapy with brain-injured children and adolescents (author's transl)].", "content": "Trampoline therapy is a useful part of the medical rehabilitation treatment of brain-injured children and adolescents, if the basic principles of accident prevention are observed. The therapy should only consist of systematically organised exercise series and be carried out by adequately trained sport teachers, medical paedagogues or physical therapists. In the \"Jugendwerk Gailingen\" an improvement of the standing balance and movement co-ordination was achieved in more than 20 patients with hemiparesis and tetraparesis. Only little progress was achieved in atactic and athetoid patients. It is important to stress the positive psychological effects of the trampoline therapy.", "contents": "[Trampoline therapy with brain-injured children and adolescents (author's transl)]. Trampoline therapy is a useful part of the medical rehabilitation treatment of brain-injured children and adolescents, if the basic principles of accident prevention are observed. The therapy should only consist of systematically organised exercise series and be carried out by adequately trained sport teachers, medical paedagogues or physical therapists. In the \"Jugendwerk Gailingen\" an improvement of the standing balance and movement co-ordination was achieved in more than 20 patients with hemiparesis and tetraparesis. Only little progress was achieved in atactic and athetoid patients. It is important to stress the positive psychological effects of the trampoline therapy.", "PMID": 959641}
{"id": "pubmed23n0032_3519", "title": "[Brachial plexus injuries caused by motor-cycle accidents- catamneses (author's transl)].", "content": "During the last eight years we examined 45 patients, mostly juvenile, with brachial plexus injuries arising from motor-cycle and moped accidents. The neurological findings, radiological picture on the myelogramme, sanguineous fluid, different electrodiagnostic examinations and sweat secretion tests provided information on the location of the lesion and the defects to be expected at a later date. However, no method permitted determination of the total extent of the damage. In some cases we observed partial, oftern considerable improvement even in the second and third post-accidential years. Follow-up studies showed the significance of the personality structure as well as social conditions with respect to vocational rehabilitation, and the danger of early resignation. Consequently, rehabilitation efforts should start immediately after the healing of the initially incurred injuries.", "contents": "[Brachial plexus injuries caused by motor-cycle accidents- catamneses (author's transl)]. During the last eight years we examined 45 patients, mostly juvenile, with brachial plexus injuries arising from motor-cycle and moped accidents. The neurological findings, radiological picture on the myelogramme, sanguineous fluid, different electrodiagnostic examinations and sweat secretion tests provided information on the location of the lesion and the defects to be expected at a later date. However, no method permitted determination of the total extent of the damage. In some cases we observed partial, oftern considerable improvement even in the second and third post-accidential years. Follow-up studies showed the significance of the personality structure as well as social conditions with respect to vocational rehabilitation, and the danger of early resignation. Consequently, rehabilitation efforts should start immediately after the healing of the initially incurred injuries.", "PMID": 959642}
{"id": "pubmed23n0032_3520", "title": "[Catamnestic studies of the value of vocational prognoses for persons with infantile brain-damage (author's transl)].", "content": "The basis of this study is the examination of 100 people who suffered from severe neurological and psychic defect syndromes. Of this group 49 were re-examined eight years later- age-range then 20 to 34. The vocational prognoses which were given at the first examination were determined according to the degree of severity of the disability and proved to be correct in 39 cases. In five cases each, the level of productivity was assessed either too high or too low. Only eleven of the sample group had an income exceeding DM 500,-45 were living either with their families or in institutions, and of the remaining four, who rented rooms, two were married. The course of life of this category of people, who suffers from such a wide range of disabilities, can be facilitated if attention is paid to the vocational prognosis before the individual starts vocational training.", "contents": "[Catamnestic studies of the value of vocational prognoses for persons with infantile brain-damage (author's transl)]. The basis of this study is the examination of 100 people who suffered from severe neurological and psychic defect syndromes. Of this group 49 were re-examined eight years later- age-range then 20 to 34. The vocational prognoses which were given at the first examination were determined according to the degree of severity of the disability and proved to be correct in 39 cases. In five cases each, the level of productivity was assessed either too high or too low. Only eleven of the sample group had an income exceeding DM 500,-45 were living either with their families or in institutions, and of the remaining four, who rented rooms, two were married. The course of life of this category of people, who suffers from such a wide range of disabilities, can be facilitated if attention is paid to the vocational prognosis before the individual starts vocational training.", "PMID": 959644}
{"id": "pubmed23n0032_3521", "title": "Contrasting mechanisms of adrenal catecholamine release by acetylcholine and high potassium.", "content": "Continuous twenty minute exposure of isolated perfused bovine adrenal glands to high potassium concentrations produced shorter durations of catecholamine release than twenty minute continuous stimulation with acetylcholine. Catecholamine release during continuous exposure to potassium decreased linearly on a semilog plot, whereas acetylcholine caused a biphasic release of catecholamine. Glands previously treated with high potassium responded poorly to acetylcholine; however, glands previously treated with acetylcholine showed a maximum response to high potassium concentrations. Full recovery of the response to acetylcholine after high potassium treatment occurred in twenty minutes. High potassium concentrations appear to evoke secretion of \"readily releasable\" material only, whereas acetylcholine has access to a larger catecholamine store in the adrenal.", "contents": "Contrasting mechanisms of adrenal catecholamine release by acetylcholine and high potassium. Continuous twenty minute exposure of isolated perfused bovine adrenal glands to high potassium concentrations produced shorter durations of catecholamine release than twenty minute continuous stimulation with acetylcholine. Catecholamine release during continuous exposure to potassium decreased linearly on a semilog plot, whereas acetylcholine caused a biphasic release of catecholamine. Glands previously treated with high potassium responded poorly to acetylcholine; however, glands previously treated with acetylcholine showed a maximum response to high potassium concentrations. Full recovery of the response to acetylcholine after high potassium treatment occurred in twenty minutes. High potassium concentrations appear to evoke secretion of \"readily releasable\" material only, whereas acetylcholine has access to a larger catecholamine store in the adrenal.", "PMID": 959649}
{"id": "pubmed23n0032_3522", "title": "In vivo dopamine release and prostaglandin E2.", "content": "Cerebroventricular perfusion of cats pretreated with H3-dopamine was used to monitor the neuronal release of dopamine. Infusion of prostaglandin E2 (PGE2, 10-6M) has no effect upon this release. Electrical stimulation in the region of the ascending dopamine containing fibers in the lateral hypothalamus results in a marked increase in H3-dopamine release. Infusion of PGE2 during electrical stimulation does not alter this release either in the presence of normal (1.16 mM) or elevated (7.5 mM) Ca++. Under the conditions of these experiments, PGE2 does not modulate dopamine release to any measureable degree.", "contents": "In vivo dopamine release and prostaglandin E2. Cerebroventricular perfusion of cats pretreated with H3-dopamine was used to monitor the neuronal release of dopamine. Infusion of prostaglandin E2 (PGE2, 10-6M) has no effect upon this release. Electrical stimulation in the region of the ascending dopamine containing fibers in the lateral hypothalamus results in a marked increase in H3-dopamine release. Infusion of PGE2 during electrical stimulation does not alter this release either in the presence of normal (1.16 mM) or elevated (7.5 mM) Ca++. Under the conditions of these experiments, PGE2 does not modulate dopamine release to any measureable degree.", "PMID": 959650}
{"id": "pubmed23n0032_3523", "title": "Comparison of the effects of R-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) propane (DOM), r-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) butane (BL-3912A) and 5-hydroxytryptamine on non-innervated vascular smooth muscle.", "content": "Isolated strips of sheep umbilical arteries contracted in the presence of R-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) butane (BL3912A). These contractions faded over an hour period and at this time BL3912A antagonized contractions to R-(-)-DOM, 5-hydroxytryptamine (5-HT) but not to angiotensin. The initial contraction produced by BL3912A was antagonized by cinanserin, a 5-HT antagonist. These experiments indicate that BL3912A can be classified as a partial agonist of 5-HT receptors in sheep umbilical arteries.", "contents": "Comparison of the effects of R-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) propane (DOM), r-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) butane (BL-3912A) and 5-hydroxytryptamine on non-innervated vascular smooth muscle. Isolated strips of sheep umbilical arteries contracted in the presence of R-(-)-2-amino-1-(2,5-dimethoxy-4-methylphenyl) butane (BL3912A). These contractions faded over an hour period and at this time BL3912A antagonized contractions to R-(-)-DOM, 5-hydroxytryptamine (5-HT) but not to angiotensin. The initial contraction produced by BL3912A was antagonized by cinanserin, a 5-HT antagonist. These experiments indicate that BL3912A can be classified as a partial agonist of 5-HT receptors in sheep umbilical arteries.", "PMID": 959651}
{"id": "pubmed23n0032_3524", "title": "Prostaglandin biosynthesis and metabolism in rat brain slices.", "content": "Prostaglandin F2alpha and E were measured in the slices and in the media after incubation of rat brain slices. About 5 to 10 times more prostaglandin F2alpha than prostaglandin E was found, most of which was secreted into the media. In the absence of oxygen or in the presence of indomethacin, prostaglandin levels in both the slices and media were reduced. Serotonin, norepinephrine, epinephrine, and DOPA, at 10(-3) M concentrations, increased the prostaglandin F2alpha/E ratio in both the media and slices. The levels of prostaglandins F2alpha found in the slices and media when the slices were incubated with prostaglandin E2 increased significantly, this increase being independent of the presence of indomethacin. This suggests that prostaglandin E2 was being converted to F2alpha by a prostaglandin E2 9-ketoreductase in the rat brain slices.", "contents": "Prostaglandin biosynthesis and metabolism in rat brain slices. Prostaglandin F2alpha and E were measured in the slices and in the media after incubation of rat brain slices. About 5 to 10 times more prostaglandin F2alpha than prostaglandin E was found, most of which was secreted into the media. In the absence of oxygen or in the presence of indomethacin, prostaglandin levels in both the slices and media were reduced. Serotonin, norepinephrine, epinephrine, and DOPA, at 10(-3) M concentrations, increased the prostaglandin F2alpha/E ratio in both the media and slices. The levels of prostaglandins F2alpha found in the slices and media when the slices were incubated with prostaglandin E2 increased significantly, this increase being independent of the presence of indomethacin. This suggests that prostaglandin E2 was being converted to F2alpha by a prostaglandin E2 9-ketoreductase in the rat brain slices.", "PMID": 959652}
{"id": "pubmed23n0032_3525", "title": "Prevention by calcium administration of reserpine action on rat brain noradrenaline stores: a reappraisal.", "content": "The conditions under which pretreatment with a calcium salt may prevent the action of reserpine on brain noradrenaline stores in the rat were investigated. The results show that only after subcutaneous administration of reserpine in the same site as a previous CaCl2 injection, was the action of reserpine prevented and reduced levels of this drug were found in the brain. Conversely, the depletion of encephalic noradrenaline following reserpine, as well as the reserpine brain concentration, were not affected by subcutaneously administered calcium chloride, when reserpine was administered either intravenously, or subcutaneously in a site different from that selected for pretreatment with the calcium salt. In essence calcium chloride, a well known irritant, acts accordingly at the site of subcutaneous administration, thus limiting by a non specific mechanism the absorption of reserpine. Under similar conditions, in fact, the absorption of a different drug, i.e. harmaline, was likewise altered. In view of these findings the significance of some studies on calcium-reserpine interaction appearing in the literature requires a reappraisal.", "contents": "Prevention by calcium administration of reserpine action on rat brain noradrenaline stores: a reappraisal. The conditions under which pretreatment with a calcium salt may prevent the action of reserpine on brain noradrenaline stores in the rat were investigated. The results show that only after subcutaneous administration of reserpine in the same site as a previous CaCl2 injection, was the action of reserpine prevented and reduced levels of this drug were found in the brain. Conversely, the depletion of encephalic noradrenaline following reserpine, as well as the reserpine brain concentration, were not affected by subcutaneously administered calcium chloride, when reserpine was administered either intravenously, or subcutaneously in a site different from that selected for pretreatment with the calcium salt. In essence calcium chloride, a well known irritant, acts accordingly at the site of subcutaneous administration, thus limiting by a non specific mechanism the absorption of reserpine. Under similar conditions, in fact, the absorption of a different drug, i.e. harmaline, was likewise altered. In view of these findings the significance of some studies on calcium-reserpine interaction appearing in the literature requires a reappraisal.", "PMID": 959653}
{"id": "pubmed23n0032_3526", "title": "The effect of CC14 on uterine ornithine decarboxylase (ODC) in the rat. Inhibition by CC14 of the induction of ODC by E2 and o,p'DDT.", "content": "The oral administration of carbon tetrachloride (CC14) to ovariectomized rats markedly inhibited the elevation of uterine decarboxylase (ODC) by estradiol-17beta (E2) and by 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2,2-trichloroethane (o,p'DDT). The inhibition of ODC induction by CC14 does not appear to be mediated by the formation of an inhibitor of ODC activity. CC14 treatment caused no observable alteration in the uterine cytosolic E2 receptor. Namely, there was no alteration by CC14 in the number of E2 binding sites, in the affinity of the receptor for E2 or in the sedimentation constant of the 8S receptor. Furthermore, the possibility that CC14 inhibition of ODC induction is a resultant of an initial stimulation of this uterine enzyme by CC14 was excluded. The potential inhibitory effect of CC14 by direct of indirect route on uterine protein synthesis is discussed.", "contents": "The effect of CC14 on uterine ornithine decarboxylase (ODC) in the rat. Inhibition by CC14 of the induction of ODC by E2 and o,p'DDT. The oral administration of carbon tetrachloride (CC14) to ovariectomized rats markedly inhibited the elevation of uterine decarboxylase (ODC) by estradiol-17beta (E2) and by 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2,2-trichloroethane (o,p'DDT). The inhibition of ODC induction by CC14 does not appear to be mediated by the formation of an inhibitor of ODC activity. CC14 treatment caused no observable alteration in the uterine cytosolic E2 receptor. Namely, there was no alteration by CC14 in the number of E2 binding sites, in the affinity of the receptor for E2 or in the sedimentation constant of the 8S receptor. Furthermore, the possibility that CC14 inhibition of ODC induction is a resultant of an initial stimulation of this uterine enzyme by CC14 was excluded. The potential inhibitory effect of CC14 by direct of indirect route on uterine protein synthesis is discussed.", "PMID": 959654}
{"id": "pubmed23n0032_3527", "title": "Preparation of guinea pig hepatic microsomes: a comparison of three techniques.", "content": "Hepatic microsomal preparations by Ca2+-aggregation and gel filtration on Sepharose 2B were compared with the product obtained by differential centrifugation, using liver from adult male guinea pigs. The conventionally-prepared microsomes were translucent and rose-red in color. Those prepared by Ca2+-aggregation were a cream or light pink color whereas the gel-filtered microsomes were opalescent and white. The specific activities of microsomal p-nitroanisole O-demethylase, aniline hydroxylase and nonspecific carboxylase were highest in the Ca2+-aggregated microsomes, intermediate in the gel-filtered preparation and lowest in the conventional microsomes. Electron microscopy revealed vesicular preparations of similar appearance though the Ca2+-aggregated microsomes were larger and contained more aggregates of free ribosomes.", "contents": "Preparation of guinea pig hepatic microsomes: a comparison of three techniques. Hepatic microsomal preparations by Ca2+-aggregation and gel filtration on Sepharose 2B were compared with the product obtained by differential centrifugation, using liver from adult male guinea pigs. The conventionally-prepared microsomes were translucent and rose-red in color. Those prepared by Ca2+-aggregation were a cream or light pink color whereas the gel-filtered microsomes were opalescent and white. The specific activities of microsomal p-nitroanisole O-demethylase, aniline hydroxylase and nonspecific carboxylase were highest in the Ca2+-aggregated microsomes, intermediate in the gel-filtered preparation and lowest in the conventional microsomes. Electron microscopy revealed vesicular preparations of similar appearance though the Ca2+-aggregated microsomes were larger and contained more aggregates of free ribosomes.", "PMID": 959655}
{"id": "pubmed23n0032_3528", "title": "Effect of various estrogen treatment schedules on antithrombin III levels.", "content": "Antithrombin III levels were determined in 18 normal indiviuals of both sexes, 24 pregnant women (at term), 20 patients treated with oral contraceptives and 21 postmenopausal patients treated with Premarin. There was no sex difference in antithrombin III levels in the untreated control group. Pregnant women at term had the lowest levels of antithrombin III. The second lowest values were exhibited by women on oral contraceptives. Both of these groups differed significantly (p less than 0.05) from the controls. Premarin treated postmenopausal women had somewhat lower levels of antithrombin III than controls but these were statistically not significant.", "contents": "Effect of various estrogen treatment schedules on antithrombin III levels. Antithrombin III levels were determined in 18 normal indiviuals of both sexes, 24 pregnant women (at term), 20 patients treated with oral contraceptives and 21 postmenopausal patients treated with Premarin. There was no sex difference in antithrombin III levels in the untreated control group. Pregnant women at term had the lowest levels of antithrombin III. The second lowest values were exhibited by women on oral contraceptives. Both of these groups differed significantly (p less than 0.05) from the controls. Premarin treated postmenopausal women had somewhat lower levels of antithrombin III than controls but these were statistically not significant.", "PMID": 959656}
{"id": "pubmed23n0032_3529", "title": "Effect of phospholipase C on lymphocyte responses to mitogen.", "content": "Peripheral lymphocytes treated with phospholipase C (phosphatidylcholine cholin phosphohydrolase E [3.1.4.3]) were examined for their response to mitogen and rosette formations. High levels of phospholipase C (greater than 0.01 unit) showed significant toxic effects on the peripheral lymphocytes as examined by the trypan blue exclusion test. This was attributable to \"impurities\" in the Cl. perfringens phospholipase C preparation since recovery of the mitogen responses were incomplete after heat inactivation of the enzyme. Active phospholipase C at 0.005 unit significantly (50%) suppressed the PHA response with little or no effect on the PWM stimulation. Similarly, a significant suppression of E rosette formation occurred with phospholipase C (0.005 u) treated lymphocytes. Suppression of similarly treated lymphocytes to EAC rosette was slight. It is suggested that the removal of phosphorylated amines from membrane surfaces affects T-cells more than B-cells and that the use of phospholipase C is a useful means of examining membrane functions of the lymphoid system.", "contents": "Effect of phospholipase C on lymphocyte responses to mitogen. Peripheral lymphocytes treated with phospholipase C (phosphatidylcholine cholin phosphohydrolase E [3.1.4.3]) were examined for their response to mitogen and rosette formations. High levels of phospholipase C (greater than 0.01 unit) showed significant toxic effects on the peripheral lymphocytes as examined by the trypan blue exclusion test. This was attributable to \"impurities\" in the Cl. perfringens phospholipase C preparation since recovery of the mitogen responses were incomplete after heat inactivation of the enzyme. Active phospholipase C at 0.005 unit significantly (50%) suppressed the PHA response with little or no effect on the PWM stimulation. Similarly, a significant suppression of E rosette formation occurred with phospholipase C (0.005 u) treated lymphocytes. Suppression of similarly treated lymphocytes to EAC rosette was slight. It is suggested that the removal of phosphorylated amines from membrane surfaces affects T-cells more than B-cells and that the use of phospholipase C is a useful means of examining membrane functions of the lymphoid system.", "PMID": 959657}
{"id": "pubmed23n0032_3530", "title": "Adenine nucleotides in thiamine deficient rat brain.", "content": "The net levels and turnover of ATP, ADP, and AMP were measured in the cortex and brainstem of thiamine deficient and control rats. In spite of a 63% decrease in pyruvate decarboxylase activity in the brainstem of severely deficient rats, metabolism of the adenine nucleotides was unaffected. These data indicate a major reserve capacity for pyruvate decarboxylase and show that the adenylate pool is not significantly altered in thiamine deficient rat brain.", "contents": "Adenine nucleotides in thiamine deficient rat brain. The net levels and turnover of ATP, ADP, and AMP were measured in the cortex and brainstem of thiamine deficient and control rats. In spite of a 63% decrease in pyruvate decarboxylase activity in the brainstem of severely deficient rats, metabolism of the adenine nucleotides was unaffected. These data indicate a major reserve capacity for pyruvate decarboxylase and show that the adenylate pool is not significantly altered in thiamine deficient rat brain.", "PMID": 959658}
{"id": "pubmed23n0032_3531", "title": "The glycogen-depleting effect of non-epinephrine on the myocardium of the six and one-half day chick embryo.", "content": "The myocardial glycogen of the six and one-half day chick embryo is lowered significantly by the in-ovo presentation of nor-epinephrine (1.0 to 20.0 micrograms/egg). This glycogen-depleting effect was neither dosage- nor time-dependent.", "contents": "The glycogen-depleting effect of non-epinephrine on the myocardium of the six and one-half day chick embryo. The myocardial glycogen of the six and one-half day chick embryo is lowered significantly by the in-ovo presentation of nor-epinephrine (1.0 to 20.0 micrograms/egg). This glycogen-depleting effect was neither dosage- nor time-dependent.", "PMID": 959659}
{"id": "pubmed23n0032_3532", "title": "Effect of cycloserine stereoisomers and theophylline on the release of tritium from 2-tritio-L-alanine.", "content": "The aearance of tritium in plasma water was measured after i.p. injection of 2-tritio-L-alanine as an index of alanine transamination in rats. L-Cycloserine (10 mg/kg) and D-cycloserine (150 mg/kg) inhibited tritium release, whereas theophylline (100 mg/kg) stimulated tritium release.", "contents": "Effect of cycloserine stereoisomers and theophylline on the release of tritium from 2-tritio-L-alanine. The aearance of tritium in plasma water was measured after i.p. injection of 2-tritio-L-alanine as an index of alanine transamination in rats. L-Cycloserine (10 mg/kg) and D-cycloserine (150 mg/kg) inhibited tritium release, whereas theophylline (100 mg/kg) stimulated tritium release.", "PMID": 959661}
{"id": "pubmed23n0032_3533", "title": "Structure-activity relationships of enkephalins in the stimulated guinea pig ileum.", "content": "A number of analogs and homologs of methionine-enkephalin (H.Tyr. Gly.Gly.Phe.Met.OH) have been synthesized by the Merrifield method of solid phase peptide synthesis. Each peptide was assayed by inhibition of electrically evoked contraction of the guinea pig ileum. The minimum sequence required for biological activity in this preparation was found to be the pentapeptide unit. Methionine was readily replaced by norleucine to give an analog with approximately 50% of the potency of the parent compound. Leucineenkephalin has about 15-20% of the potency of the methionine dervative. Modification of the N-terminal tyrosine moiety (i.e. substitution by phenylalanine or removal of the amino group) practically abolished activity. Incorporation of O-methyl tyrosine into the peptide reduced potency to 1% of the parent compound. The significance of these and other findings in terms of the topography of the guinea pig ileum receptor site is discussed.", "contents": "Structure-activity relationships of enkephalins in the stimulated guinea pig ileum. A number of analogs and homologs of methionine-enkephalin (H.Tyr. Gly.Gly.Phe.Met.OH) have been synthesized by the Merrifield method of solid phase peptide synthesis. Each peptide was assayed by inhibition of electrically evoked contraction of the guinea pig ileum. The minimum sequence required for biological activity in this preparation was found to be the pentapeptide unit. Methionine was readily replaced by norleucine to give an analog with approximately 50% of the potency of the parent compound. Leucineenkephalin has about 15-20% of the potency of the methionine dervative. Modification of the N-terminal tyrosine moiety (i.e. substitution by phenylalanine or removal of the amino group) practically abolished activity. Incorporation of O-methyl tyrosine into the peptide reduced potency to 1% of the parent compound. The significance of these and other findings in terms of the topography of the guinea pig ileum receptor site is discussed.", "PMID": 959662}
{"id": "pubmed23n0032_3534", "title": "Simultaneous measurement of plasma levels of d-Propoxyphene and l-Propoxyphene using stable isotope labels and mass fragmentography.", "content": "When racemic propoxyphene was administered orally to dogs, plasma levels of d-propoxyphene were higher than those of l-propoxyphene. The half-life of d-propoxyphene was also longer than that of the l-isomer. Measurements were performed by labeling one isomer of a racemic mixture with deuterium and then measuring d- vs. l- ratios by mass fragmentography. An intravenous experiment was also performed and confirmed the preferential uptake of the levo isomer. To do these experiments successfully propoxyphene isomers were labeled on the benzyl methylene with deuterium atoms. In vivo studies demonstrated that this labeling position was sufficiently far removed from the point of metabolic attack in the molecule so that no interfering isotope effect existed. The methodology outlined in this paper will be of great value in the study of the disposition of the individual isomers of racemic drugs.", "contents": "Simultaneous measurement of plasma levels of d-Propoxyphene and l-Propoxyphene using stable isotope labels and mass fragmentography. When racemic propoxyphene was administered orally to dogs, plasma levels of d-propoxyphene were higher than those of l-propoxyphene. The half-life of d-propoxyphene was also longer than that of the l-isomer. Measurements were performed by labeling one isomer of a racemic mixture with deuterium and then measuring d- vs. l- ratios by mass fragmentography. An intravenous experiment was also performed and confirmed the preferential uptake of the levo isomer. To do these experiments successfully propoxyphene isomers were labeled on the benzyl methylene with deuterium atoms. In vivo studies demonstrated that this labeling position was sufficiently far removed from the point of metabolic attack in the molecule so that no interfering isotope effect existed. The methodology outlined in this paper will be of great value in the study of the disposition of the individual isomers of racemic drugs.", "PMID": 959663}
{"id": "pubmed23n0032_3535", "title": "Potentiation of isoproterenol effect by ascorbic acid.", "content": "Intraduodenal administration of isoproterenol together with ascorbic acid caused a much greater increase in the heart rate and pulse pressure, and a greater decrease in the mean blood pressure of anesthetized dogs than was observed after intraduodenal administration of isoproterenol alone. No potentiating effect was observed when isoproterenol and the vitamin were injected intravenously.", "contents": "Potentiation of isoproterenol effect by ascorbic acid. Intraduodenal administration of isoproterenol together with ascorbic acid caused a much greater increase in the heart rate and pulse pressure, and a greater decrease in the mean blood pressure of anesthetized dogs than was observed after intraduodenal administration of isoproterenol alone. No potentiating effect was observed when isoproterenol and the vitamin were injected intravenously.", "PMID": 959664}
{"id": "pubmed23n0032_3536", "title": "Effects of cannabinoids on the perfused rat heart.", "content": "In the isolated perfused rat heart cardiac rate and contractility are directly affected by four naturally occurring cannabis constituents, delta9-tetrahydrocannabinol, delta8-tetrahydrocannabinol, cannabinol and cannabidiol. All the drugs depressed myocardial contractility. Their effect on rate, however, was not uniform: delta8-Tetrahydrocannabinol produced cardiac arrhythmias but no other consistent change; both delta9-tetrahydrocannabinol and cannabinol caused tachycardia; but cannabidiol produced bradycardia, arrhythmias and asystole. In addition, as a consequent of the dispostion of the cannabinoids to accumulate in the isolated heart, high tissue concentrations appeared in conjunction with the direct cardiac effects.", "contents": "Effects of cannabinoids on the perfused rat heart. In the isolated perfused rat heart cardiac rate and contractility are directly affected by four naturally occurring cannabis constituents, delta9-tetrahydrocannabinol, delta8-tetrahydrocannabinol, cannabinol and cannabidiol. All the drugs depressed myocardial contractility. Their effect on rate, however, was not uniform: delta8-Tetrahydrocannabinol produced cardiac arrhythmias but no other consistent change; both delta9-tetrahydrocannabinol and cannabinol caused tachycardia; but cannabidiol produced bradycardia, arrhythmias and asystole. In addition, as a consequent of the dispostion of the cannabinoids to accumulate in the isolated heart, high tissue concentrations appeared in conjunction with the direct cardiac effects.", "PMID": 959665}
{"id": "pubmed23n0032_3537", "title": "Environmental interaction of lead and cadmium on reproduction and metabolism of male rats.", "content": "To study the environmental interaction of lead and cadmium on reproduction and metabolism 70 male sprague Dawley rats were divided equally into 7 goups. Goups 1 and 2 served as controls, 3 and 4 were injected daily with 50 and 250 ug of lead respectively, 5 and 6 with 50 and 250 ug of cadmium respectively and group 7 with 25 ug of both lead and cadmium. After 70 days of injections rats were sacrificed. In group 6 cadmium injection caused enlargement of adrenal, liver, kidney and spleen but retarded growth, reduction in size of prostate, testes, epididymis and hepatic enzyme activity. Groups 4 and6 had the highest and most significant mineral concentration in blood and liver. Testes histology of group 7 showed an absence of spermatogenesis in some seminiferous tubules indicating that low levels of lead and cadmium together have a more synergetic damaging effect on rat testes than higher levels of lead or cadmium alone. This study suggests that blood mineral levels should be used with other more sensitive clinical tests to assess the toxicity picture in humans.", "contents": "Environmental interaction of lead and cadmium on reproduction and metabolism of male rats. To study the environmental interaction of lead and cadmium on reproduction and metabolism 70 male sprague Dawley rats were divided equally into 7 goups. Goups 1 and 2 served as controls, 3 and 4 were injected daily with 50 and 250 ug of lead respectively, 5 and 6 with 50 and 250 ug of cadmium respectively and group 7 with 25 ug of both lead and cadmium. After 70 days of injections rats were sacrificed. In group 6 cadmium injection caused enlargement of adrenal, liver, kidney and spleen but retarded growth, reduction in size of prostate, testes, epididymis and hepatic enzyme activity. Groups 4 and6 had the highest and most significant mineral concentration in blood and liver. Testes histology of group 7 showed an absence of spermatogenesis in some seminiferous tubules indicating that low levels of lead and cadmium together have a more synergetic damaging effect on rat testes than higher levels of lead or cadmium alone. This study suggests that blood mineral levels should be used with other more sensitive clinical tests to assess the toxicity picture in humans.", "PMID": 959666}
{"id": "pubmed23n0032_3538", "title": "Bioenergetics in clinical medicine. IX. Gingival and leucocytic deficiencies of coenzyme Q10 in patients with periodontal disease.", "content": "The specific activities of the succinate dehydrogenase-coenzyme Q10 reductase in mitochondria were determined for patients from a normal periodontal practice. The criteria for selection were patients having a bone score of 1.0-4.0 and a pocket depth of 2.5-5.2 mm. All 29 patients showed a deficiency of 20-63% of CoQ10-enzyme activity in gingival biopsies. The mean value was elevated (P less than 0.001) over that of controls. For corresponding blood samples, 24/28 (86%) showed deficiencies of 20-66% and a higher (P less than 0.001) mean value than that of controls. Periodontal patients frequently have significant gingival and leucocytic deficiencies of CoQ10. The leucocytic deficiency indicates a systemic nutritional imbalance and is not likely caused by neglected oral hygiene. A gingival deficiency could predispose this tissue to periodontitis and this disease could even augment the deficiency. These results support previously suggested adjunctive use of CoQ10 with oral hygiene for improved treatment presumably through bioenergetics.", "contents": "Bioenergetics in clinical medicine. IX. Gingival and leucocytic deficiencies of coenzyme Q10 in patients with periodontal disease. The specific activities of the succinate dehydrogenase-coenzyme Q10 reductase in mitochondria were determined for patients from a normal periodontal practice. The criteria for selection were patients having a bone score of 1.0-4.0 and a pocket depth of 2.5-5.2 mm. All 29 patients showed a deficiency of 20-63% of CoQ10-enzyme activity in gingival biopsies. The mean value was elevated (P less than 0.001) over that of controls. For corresponding blood samples, 24/28 (86%) showed deficiencies of 20-66% and a higher (P less than 0.001) mean value than that of controls. Periodontal patients frequently have significant gingival and leucocytic deficiencies of CoQ10. The leucocytic deficiency indicates a systemic nutritional imbalance and is not likely caused by neglected oral hygiene. A gingival deficiency could predispose this tissue to periodontitis and this disease could even augment the deficiency. These results support previously suggested adjunctive use of CoQ10 with oral hygiene for improved treatment presumably through bioenergetics.", "PMID": 959667}
{"id": "pubmed23n0032_3539", "title": "Intersubject variation of warfarin binding to protein in serum of normal subjects.", "content": "The protein binding of warfarin was determined in the serum of 18 normal adult subjects (8 males and 10 females). A statistically significant negative correlation between free fraction of warfarin and albumin concentration was found. The free fraction of warfarin in the serum of women was significantly larger than that in the serum of men, apparently due to the lower albumin concentration in the serum of women.", "contents": "Intersubject variation of warfarin binding to protein in serum of normal subjects. The protein binding of warfarin was determined in the serum of 18 normal adult subjects (8 males and 10 females). A statistically significant negative correlation between free fraction of warfarin and albumin concentration was found. The free fraction of warfarin in the serum of women was significantly larger than that in the serum of men, apparently due to the lower albumin concentration in the serum of women.", "PMID": 959669}
{"id": "pubmed23n0032_3540", "title": "Effect of phenylbutazone on the metabolic disposition of warfarin in the dog.", "content": "Phenylbutazone has been shown to modify the physiologic disposition of warfarin in the dog. Dogs pretreated with phenylbutazone for a week, and permitted to remain drug-free for an additional week, eliminated warfarin from plasma 2-3 times faster than non-pretreated controls with an average half-life of 7.8 hours. In-vitro hepatic microsomal metabolism of warfarin and cytochrome P-450 content were greater for hepatic microsomes derived from phenylbutazone pretreated animals.", "contents": "Effect of phenylbutazone on the metabolic disposition of warfarin in the dog. Phenylbutazone has been shown to modify the physiologic disposition of warfarin in the dog. Dogs pretreated with phenylbutazone for a week, and permitted to remain drug-free for an additional week, eliminated warfarin from plasma 2-3 times faster than non-pretreated controls with an average half-life of 7.8 hours. In-vitro hepatic microsomal metabolism of warfarin and cytochrome P-450 content were greater for hepatic microsomes derived from phenylbutazone pretreated animals.", "PMID": 959670}
{"id": "pubmed23n0032_3541", "title": "Effect of growth hormone on weight and mitochondrial protein content of rat heart.", "content": "In male rats hypophysectomy reduced the wet weight of hearts per 100 g body weight. The yield of mitochondrial protein per g heart was also decreased which is in contrast to that in liver. Adiminstration of bGH to hypophysectomized rats reversed these changes towards normal. It appears that some of the effects of growth hormone may vary in different tissues.", "contents": "Effect of growth hormone on weight and mitochondrial protein content of rat heart. In male rats hypophysectomy reduced the wet weight of hearts per 100 g body weight. The yield of mitochondrial protein per g heart was also decreased which is in contrast to that in liver. Adiminstration of bGH to hypophysectomized rats reversed these changes towards normal. It appears that some of the effects of growth hormone may vary in different tissues.", "PMID": 959673}
{"id": "pubmed23n0032_3542", "title": "Influence of diaphragmatic contraction and expiratory flow on the pattern of lung emptying.", "content": "Transdiaphragmatic pressure (Pdi) and expiratory flow (V) were monitored during vital capacity single breath N2 washouts in 7 seated subjects. Transient increases in V were produced (1) actively, by subjects increasing mouth pressure while expiring through a constant resistance of (2) passively, by the operator transiently decreasing the resistance. Voluntary contraction of the diaphragm (increased Pdi) was achieved when abdominal muscles were tensed while maintaining V constant. In 5 subjects a transient increase in Pdi of 25-150 cm H2O consistently produced a transient increase in expired N2 concentration of 1.80 +/- 0.06% (Mean +/- 1 SE); in 1 subject N2 concentration decreased by 0.8% to 2.7% N2, and in one subject the alveolar plateau was uninfluenced by changes in Pdi. Passive increases in V up to 21/sec had no effect on FEN2 in any of the subjects. Active increase in V changed FEN2 only when associated with increases in Pdi. Qualitatively similar results were obtained during helium (He) bolus washouts. However, whereas diaphragmatic contraction, maintained throughout expiration, had no measurable influence on the N2 washout, it changed the slope of the He alveolar plateau in 6 out of 7 subjects. We conclude that in normal subjects the alveolar N2 plateau is relatively insensitive to flow variations up to 21/sec. The fluctuations in FEN2 observed when the expiratory flow is varied are due to concomittant changes in Pdi. We propose that diaphragmatic contraction changes the pattern of lung emptying by altering the vertical gradient of pleural pressure.", "contents": "Influence of diaphragmatic contraction and expiratory flow on the pattern of lung emptying. Transdiaphragmatic pressure (Pdi) and expiratory flow (V) were monitored during vital capacity single breath N2 washouts in 7 seated subjects. Transient increases in V were produced (1) actively, by subjects increasing mouth pressure while expiring through a constant resistance of (2) passively, by the operator transiently decreasing the resistance. Voluntary contraction of the diaphragm (increased Pdi) was achieved when abdominal muscles were tensed while maintaining V constant. In 5 subjects a transient increase in Pdi of 25-150 cm H2O consistently produced a transient increase in expired N2 concentration of 1.80 +/- 0.06% (Mean +/- 1 SE); in 1 subject N2 concentration decreased by 0.8% to 2.7% N2, and in one subject the alveolar plateau was uninfluenced by changes in Pdi. Passive increases in V up to 21/sec had no effect on FEN2 in any of the subjects. Active increase in V changed FEN2 only when associated with increases in Pdi. Qualitatively similar results were obtained during helium (He) bolus washouts. However, whereas diaphragmatic contraction, maintained throughout expiration, had no measurable influence on the N2 washout, it changed the slope of the He alveolar plateau in 6 out of 7 subjects. We conclude that in normal subjects the alveolar N2 plateau is relatively insensitive to flow variations up to 21/sec. The fluctuations in FEN2 observed when the expiratory flow is varied are due to concomittant changes in Pdi. We propose that diaphragmatic contraction changes the pattern of lung emptying by altering the vertical gradient of pleural pressure.", "PMID": 959674}
{"id": "pubmed23n0032_3543", "title": "Pulmonary arteriovenous anastomoses in the avian lung: do they exist?", "content": "A search for pulmonary arteriovenous anastomoses was made in 15 adult domestic fowls using Lycopodium spores and microspheres. The diameter of the spores and microspheres ranged from about 10 to 33 mum. To dilate any pulmonary arteriovenous anastomoses, the birds were warmed to induce panting, killed with chloroform, or injected intravenously with papavarine. The spores or microspheres were injected either into the jugular vein under anaesthesia, or into the pulmonary artery after death. After the pulmonary arterial injections, the effluent from the pulmonary vein, and histological sections of the lungs, were examined for spores or microspheres. When injections were made into the jugular vein, blood smears from the pulmonary veins, left atrium, and the aorta, as well as histological sections of the lungs and other organs were inspected. The results of all these experiments showed that no spores or microspheres were ever found on the venous side of the pulmonary circulation, indicating absence of pulmonary arteriovenous anastomoses.", "contents": "Pulmonary arteriovenous anastomoses in the avian lung: do they exist? A search for pulmonary arteriovenous anastomoses was made in 15 adult domestic fowls using Lycopodium spores and microspheres. The diameter of the spores and microspheres ranged from about 10 to 33 mum. To dilate any pulmonary arteriovenous anastomoses, the birds were warmed to induce panting, killed with chloroform, or injected intravenously with papavarine. The spores or microspheres were injected either into the jugular vein under anaesthesia, or into the pulmonary artery after death. After the pulmonary arterial injections, the effluent from the pulmonary vein, and histological sections of the lungs, were examined for spores or microspheres. When injections were made into the jugular vein, blood smears from the pulmonary veins, left atrium, and the aorta, as well as histological sections of the lungs and other organs were inspected. The results of all these experiments showed that no spores or microspheres were ever found on the venous side of the pulmonary circulation, indicating absence of pulmonary arteriovenous anastomoses.", "PMID": 959675}
{"id": "pubmed23n0032_3544", "title": "Pattern of breathing during hypoxia or hypercapnia of the awake or anesthetized cat.", "content": "The breathing patterns during hypoxia and hypercapnia are similar in anesthetized cats but are qualitatively different in awake cats. The differences seen in the awake animals can be explained by either the central depressive effect of hypoxia or by a specific effect of hypercapnia on supra-pontine structures. The Breuer-Hering reflex sensitivity, i.e. the VT-TI relationship, appears, in the awake cat, quite similar to that recently described in man. The inspiratory activity is shown to be controlled by mechanisms dependent on the nature of the respiratory stimulation. The recent model proposed for the control of inspiration during anesthesia must be modified to account for the results observed in awake animal.", "contents": "Pattern of breathing during hypoxia or hypercapnia of the awake or anesthetized cat. The breathing patterns during hypoxia and hypercapnia are similar in anesthetized cats but are qualitatively different in awake cats. The differences seen in the awake animals can be explained by either the central depressive effect of hypoxia or by a specific effect of hypercapnia on supra-pontine structures. The Breuer-Hering reflex sensitivity, i.e. the VT-TI relationship, appears, in the awake cat, quite similar to that recently described in man. The inspiratory activity is shown to be controlled by mechanisms dependent on the nature of the respiratory stimulation. The recent model proposed for the control of inspiration during anesthesia must be modified to account for the results observed in awake animal.", "PMID": 959676}
{"id": "pubmed23n0032_3545", "title": "The interaction of the lung and chest wall in dogs.", "content": "The deformation of the lung by the chest wall was observed on radiographs of the ribs and interspaces and on morphologic preparations of samples cut from the frozen thorax. The effect of these deformities on the distribution of ventilation and regional lung volume of the underlying lung was determined using Xenon 133. We found that ribs and interspaces deform the lung surface with different radii of curvature, suggesting local differences in pleural pressure and that traction on the thoracic limb caused deformities in the underlying interspaces which were similar to those produced by 20 cm H2O mouth pressure. Xenon 133 showed that these changes in pressure had no effect on the regional volume or on ventilation distribution in the underlying lung. We conclude that changes in pleural pressure produced by localized deformities exert their effect close to the deformity and that the underlying lung responds to the average pressure exerted over a much larger area of the pleural surface.", "contents": "The interaction of the lung and chest wall in dogs. The deformation of the lung by the chest wall was observed on radiographs of the ribs and interspaces and on morphologic preparations of samples cut from the frozen thorax. The effect of these deformities on the distribution of ventilation and regional lung volume of the underlying lung was determined using Xenon 133. We found that ribs and interspaces deform the lung surface with different radii of curvature, suggesting local differences in pleural pressure and that traction on the thoracic limb caused deformities in the underlying interspaces which were similar to those produced by 20 cm H2O mouth pressure. Xenon 133 showed that these changes in pressure had no effect on the regional volume or on ventilation distribution in the underlying lung. We conclude that changes in pleural pressure produced by localized deformities exert their effect close to the deformity and that the underlying lung responds to the average pressure exerted over a much larger area of the pleural surface.", "PMID": 959677}
{"id": "pubmed23n0032_3546", "title": "Lung tissue plasticity: morphometric analysis of anisotropic strain in liquid filled lungs.", "content": "Lungs of rats were fixed at different inflation pressures (Ptp) during liquid filling with the pulmonary vessels tied to prevent vascular volume change after fixation had begun. Morphometric analysis showed that alveolar surface varied as a alveolar volume (Va) to the power 0.82, while the arithmetic mean tissue thickness varied as Va-0.2. This is interpreted as evidence for anisotropic expansion. Capillary volume (Vc) was found to increase from zero Ptp to a maximum at Ptp = 2 cm H2O then decrease as Va increased. Morphometric diffusion capacity of the membrane component increased as Va0.59 while that for whole lung (DL)paralleled the change in Vc. Alveolar capillary tissue unfolding is described as the main factor accounting for anistropic expansion of alveolar surface and for capillary configuration. The absolute values of Vc and DL were lower by 60% and 50%, respectively, compared with values obtained by standard instillation fixation methods and this is suggested could account for previous discrepancres between morphometric and physiologic estimates of DL.", "contents": "Lung tissue plasticity: morphometric analysis of anisotropic strain in liquid filled lungs. Lungs of rats were fixed at different inflation pressures (Ptp) during liquid filling with the pulmonary vessels tied to prevent vascular volume change after fixation had begun. Morphometric analysis showed that alveolar surface varied as a alveolar volume (Va) to the power 0.82, while the arithmetic mean tissue thickness varied as Va-0.2. This is interpreted as evidence for anisotropic expansion. Capillary volume (Vc) was found to increase from zero Ptp to a maximum at Ptp = 2 cm H2O then decrease as Va increased. Morphometric diffusion capacity of the membrane component increased as Va0.59 while that for whole lung (DL)paralleled the change in Vc. Alveolar capillary tissue unfolding is described as the main factor accounting for anistropic expansion of alveolar surface and for capillary configuration. The absolute values of Vc and DL were lower by 60% and 50%, respectively, compared with values obtained by standard instillation fixation methods and this is suggested could account for previous discrepancres between morphometric and physiologic estimates of DL.", "PMID": 959678}
{"id": "pubmed23n0032_3547", "title": "Effect of antigen challenge on sensitized guinea pig trachea.", "content": "Isolated tracheal preparations (ITP) were obtained from control guinea pigs and animals with acute and chronic experimental asthma (AA and CHA). It was observed that after in vitro antigen challenge, in addition to the classic Schultz-Dale response (the immediate increase in the tone of sensitized airway smooth muscle), a significant increase of the active state of airway smooth muscle was seen. This active state was observed as the potentiated, phasic, mechanical activities of ITP and was evaluated by both maximum active tension (AT max) and maximum rate of tension development (dT/dt). No apparent functional differences in the tonic response or the active state of airway smooth muscle between AA and CHA animals were found. Tracheas isolated from animals with CHA demonstrated smooth muscle hypertrophy as measured by wet, dry and relative weights of the tracheas. The repeated administration of antigen into the experimental bath was ineffective in inducing any physiological changes. The administration of histamine to ITP from control animals induced changes of mechanical activities of trachea comparable to those seen in both AA and CHA animals.", "contents": "Effect of antigen challenge on sensitized guinea pig trachea. Isolated tracheal preparations (ITP) were obtained from control guinea pigs and animals with acute and chronic experimental asthma (AA and CHA). It was observed that after in vitro antigen challenge, in addition to the classic Schultz-Dale response (the immediate increase in the tone of sensitized airway smooth muscle), a significant increase of the active state of airway smooth muscle was seen. This active state was observed as the potentiated, phasic, mechanical activities of ITP and was evaluated by both maximum active tension (AT max) and maximum rate of tension development (dT/dt). No apparent functional differences in the tonic response or the active state of airway smooth muscle between AA and CHA animals were found. Tracheas isolated from animals with CHA demonstrated smooth muscle hypertrophy as measured by wet, dry and relative weights of the tracheas. The repeated administration of antigen into the experimental bath was ineffective in inducing any physiological changes. The administration of histamine to ITP from control animals induced changes of mechanical activities of trachea comparable to those seen in both AA and CHA animals.", "PMID": 959679}
{"id": "pubmed23n0032_3548", "title": "The effect of lung reflexes on the pattern of breathing in cats.", "content": "Tidal volume (VT), and inspiratory (tI) AND EXPIRATORY (TE) durations have been measured in anaesthetized cats on stimulation of alveolar type J (nociceptive) and lung irritant receptors by intravenous injections of phenyl diguanide and of histamine acid phosphate respectively. The reflexes were studied during eupnoea, hypercapnic and hypoxic hyperpnoea, during rebreathing from hyperventilation apnoea and at different body temperatures. In all conditions the drugs caused rapid shallow breathing with reduction in VT, tI and tE. The VT/tI relationship for injection of the drugs was different from that caused by hypercapnic stimulation of breathing, but the tI/tE relationship was proportionally similar for all conditions. Recording single unit activity from phrenic motor fibres showed that the lung reflexes had little action on the initial frequency of discharge of the fibres, but cut short the discharge earlier than for the controls. The results are interpreted in terms of the ways in which lung reflexes can modify the pattern of breathing.", "contents": "The effect of lung reflexes on the pattern of breathing in cats. Tidal volume (VT), and inspiratory (tI) AND EXPIRATORY (TE) durations have been measured in anaesthetized cats on stimulation of alveolar type J (nociceptive) and lung irritant receptors by intravenous injections of phenyl diguanide and of histamine acid phosphate respectively. The reflexes were studied during eupnoea, hypercapnic and hypoxic hyperpnoea, during rebreathing from hyperventilation apnoea and at different body temperatures. In all conditions the drugs caused rapid shallow breathing with reduction in VT, tI and tE. The VT/tI relationship for injection of the drugs was different from that caused by hypercapnic stimulation of breathing, but the tI/tE relationship was proportionally similar for all conditions. Recording single unit activity from phrenic motor fibres showed that the lung reflexes had little action on the initial frequency of discharge of the fibres, but cut short the discharge earlier than for the controls. The results are interpreted in terms of the ways in which lung reflexes can modify the pattern of breathing.", "PMID": 959680}
{"id": "pubmed23n0032_3549", "title": "Functional significance of thoracic vagal branches in the chicken.", "content": "One minute electrical stimulation was used to excite the right and left cervical vagi as well as specific points on, or branches of, the left thoracic vagus. Respiratory, heart rate and blood pressure responses were observed with the nerves intact and cut. Stimulation of either intact cervical vagus produced apnea, bradycardia and blood pressure depression. Stimulation of the cut ends after nerve section demonstrated that the heart rate and blood pressure effects were efferent and the respiratory change was afferent. No responses were observed due to stimulation of the vagus caudal to the lungs. Stimulation of cardiac branches reduced heart rate and blood pressure but did not produce significant respiratory effects. Middle and anterior pulmonary branches were found to contribute only to respiratory changes through afferent nerves. Sudden, sustained reduction of CO2 in the airways produced immediate, sustained apnea. The data suggest that CO2 sensitive thoracic receptors important in regulation of respiration are confined primarily to the lungs and that these receptors play no direct role in cardiovascular function.", "contents": "Functional significance of thoracic vagal branches in the chicken. One minute electrical stimulation was used to excite the right and left cervical vagi as well as specific points on, or branches of, the left thoracic vagus. Respiratory, heart rate and blood pressure responses were observed with the nerves intact and cut. Stimulation of either intact cervical vagus produced apnea, bradycardia and blood pressure depression. Stimulation of the cut ends after nerve section demonstrated that the heart rate and blood pressure effects were efferent and the respiratory change was afferent. No responses were observed due to stimulation of the vagus caudal to the lungs. Stimulation of cardiac branches reduced heart rate and blood pressure but did not produce significant respiratory effects. Middle and anterior pulmonary branches were found to contribute only to respiratory changes through afferent nerves. Sudden, sustained reduction of CO2 in the airways produced immediate, sustained apnea. The data suggest that CO2 sensitive thoracic receptors important in regulation of respiration are confined primarily to the lungs and that these receptors play no direct role in cardiovascular function.", "PMID": 959681}
{"id": "pubmed23n0032_3550", "title": "[Arteries of the human thalamus. I. Artery and polar thalamic territory of the posterior communicating artery].", "content": "The posterior communicating artery participates to the thalamic vascularization in 60 to 70 p. 100 of the brains. It does by one unic, well characterized artery, the polar artery. Its territory comprises the rostral pole of the lateral region up to the mamillo thalamic fasciculus.", "contents": "[Arteries of the human thalamus. I. Artery and polar thalamic territory of the posterior communicating artery]. The posterior communicating artery participates to the thalamic vascularization in 60 to 70 p. 100 of the brains. It does by one unic, well characterized artery, the polar artery. Its territory comprises the rostral pole of the lateral region up to the mamillo thalamic fasciculus.", "PMID": 959701}
{"id": "pubmed23n0032_3551", "title": "[Arteries of the human thalamus. II. Arteries and paramedian thalamic territory of the communicating basilar artery].", "content": "The basilar communicating artery (or mesencephalic artery) gives off more often one than two paramedian thalamic arteries. The paramedian territory, infero-medial, has a variable extent. It can include the polar territory when the polar artery does not exist. The paramedian artery arises as often from the controlateral basilar communicating artery than from the ipsilateral one. One basilar communicating artery may so have a very extensive bilateral territory.", "contents": "[Arteries of the human thalamus. II. Arteries and paramedian thalamic territory of the communicating basilar artery]. The basilar communicating artery (or mesencephalic artery) gives off more often one than two paramedian thalamic arteries. The paramedian territory, infero-medial, has a variable extent. It can include the polar territory when the polar artery does not exist. The paramedian artery arises as often from the controlateral basilar communicating artery than from the ipsilateral one. One basilar communicating artery may so have a very extensive bilateral territory.", "PMID": 959702}
{"id": "pubmed23n0032_3552", "title": "[Study of 5 cases of ocular myopathy].", "content": "The authors reported five cases of eyelid ptosis with progesssive external ophthalmoplegia. They emphasize infra-clinic diffusion, fatigability and variability impairment of extra-ocular muscles; coexistence of myogenic E.M.G. and single potential recurring at high frequency in the same patient; mitochondrial abnormalities in type I fibers, intense oxydative activity in superior oblique muscle and normal motor end-plates.", "contents": "[Study of 5 cases of ocular myopathy]. The authors reported five cases of eyelid ptosis with progesssive external ophthalmoplegia. They emphasize infra-clinic diffusion, fatigability and variability impairment of extra-ocular muscles; coexistence of myogenic E.M.G. and single potential recurring at high frequency in the same patient; mitochondrial abnormalities in type I fibers, intense oxydative activity in superior oblique muscle and normal motor end-plates.", "PMID": 959703}
{"id": "pubmed23n0032_3553", "title": "[Elementary logic and semantic fields in aphasia].", "content": "Modern linguistics, like genetic psychology, postulate a non-linguistic basis for the structurisation of the semantic fields. If such is the case, it should be possible to observe in aphasia, where semantic deficiency predominates, a related disturbance in the semantic fields and logic. Paraxodically, such disturbances have been observed both in Broca's aphasia and in the predominantly semantic aphasia of Wernicke. A qualitative analysis shows that quantitatively identical results in the semantic aphasias of Broca and Wernicke are the result of phenomenological convergence. The related disturbance in the semantic fields and logic appears characteristic of the semantic deficiency which, in aphasic semiology, is marked by the substitution of one word for another. These finding support the theory of the relative autonomy of phonemic and semantic disorders in Wernicke's aphasia. They confirm the linguistic and genetic theory that the structure of vocabulatory is based on extralinguistic factors. They do not however provide an explantation for semantic disorders in aphasia. Further research is required to discover on what functional system articulation of the symbolic function and general operative capacity is based.", "contents": "[Elementary logic and semantic fields in aphasia]. Modern linguistics, like genetic psychology, postulate a non-linguistic basis for the structurisation of the semantic fields. If such is the case, it should be possible to observe in aphasia, where semantic deficiency predominates, a related disturbance in the semantic fields and logic. Paraxodically, such disturbances have been observed both in Broca's aphasia and in the predominantly semantic aphasia of Wernicke. A qualitative analysis shows that quantitatively identical results in the semantic aphasias of Broca and Wernicke are the result of phenomenological convergence. The related disturbance in the semantic fields and logic appears characteristic of the semantic deficiency which, in aphasic semiology, is marked by the substitution of one word for another. These finding support the theory of the relative autonomy of phonemic and semantic disorders in Wernicke's aphasia. They confirm the linguistic and genetic theory that the structure of vocabulatory is based on extralinguistic factors. They do not however provide an explantation for semantic disorders in aphasia. Further research is required to discover on what functional system articulation of the symbolic function and general operative capacity is based.", "PMID": 959704}
{"id": "pubmed23n0032_3554", "title": "Cancer of the large intestine in man: progress and problems.", "content": "This article reviews the high incidence of colon cancer in Western countries; the contribution of cell biology and biochemistry to our knowledge of the behaviour of the disease is discussed. The use of a series of markers to obtain early evidence of metastasis and to make the prognosis is described. Some suggestions are made how to obtain improvements in the survival percentages.", "contents": "Cancer of the large intestine in man: progress and problems. This article reviews the high incidence of colon cancer in Western countries; the contribution of cell biology and biochemistry to our knowledge of the behaviour of the disease is discussed. The use of a series of markers to obtain early evidence of metastasis and to make the prognosis is described. Some suggestions are made how to obtain improvements in the survival percentages.", "PMID": 959710}
{"id": "pubmed23n0032_3555", "title": "[Allergic reaction to patent blue at lymphography (author's transl)].", "content": "The authors report three cases of severe and three cases of mild allergic reactions due to Patent Blue being used for the visualization of the lymphatic vessels at lymphography. The therapy and the possibilities of the prevention of this complication are discussed particularly.", "contents": "[Allergic reaction to patent blue at lymphography (author's transl)]. The authors report three cases of severe and three cases of mild allergic reactions due to Patent Blue being used for the visualization of the lymphatic vessels at lymphography. The therapy and the possibilities of the prevention of this complication are discussed particularly.", "PMID": 959714}
{"id": "pubmed23n0032_3556", "title": "Hodgkin's-like disease of the thymus. Clinical, surgical and morphological correlations.", "content": "The histogenesis and relationship of so-called \"granulomatous thymoma\" to Hodgkin's disease is still a matter of controversy. Three of these lesions were selected from 43 thymomas and their clinico-pathological patterns are described. Two cases were diagnosed as Hodgkin's disease of the mediastinum because of the absence of epithelial structures of thymic origin. In one case, together with the morphological aspects of Hodgkin's disease, we noticed epithelial structures indicating a primary neoplastic lesion in the thymus. Accordingly we conclude that the term \"Hodgkin's disease of the thymus\" must be limited only to those lesions of the mediastinum in which structures of thymic origin are present. Other mediastinal Hodgkin's lesions, even in the presence of clinical, radiological and surgical evidence of thymic origin but in which it is not possible to observe such thymic structures, must be classified as Hodgkin's disease localized to the anterior mediastinum.", "contents": "Hodgkin's-like disease of the thymus. Clinical, surgical and morphological correlations. The histogenesis and relationship of so-called \"granulomatous thymoma\" to Hodgkin's disease is still a matter of controversy. Three of these lesions were selected from 43 thymomas and their clinico-pathological patterns are described. Two cases were diagnosed as Hodgkin's disease of the mediastinum because of the absence of epithelial structures of thymic origin. In one case, together with the morphological aspects of Hodgkin's disease, we noticed epithelial structures indicating a primary neoplastic lesion in the thymus. Accordingly we conclude that the term \"Hodgkin's disease of the thymus\" must be limited only to those lesions of the mediastinum in which structures of thymic origin are present. Other mediastinal Hodgkin's lesions, even in the presence of clinical, radiological and surgical evidence of thymic origin but in which it is not possible to observe such thymic structures, must be classified as Hodgkin's disease localized to the anterior mediastinum.", "PMID": 959711}
{"id": "pubmed23n0032_3557", "title": "Viper venoms and coumarin-induced prothrombin. A comparison of several one-stage methods employing three different venoms as thromboplastins.", "content": "Three venoms obtained from three vipers, namely Echis carinatus, Notechis scutatus scutatus and Oxyuranus scutellatus, have been used as thromboplastin in a one-stage assay of coumarin-induced prothrombin. Regardless of the venom used, prothrombin resulted to be low in coumarin-treated patients. The mean values obtained were 27.2, 33.6, and 24.2%, respectively. These values were comparable to those obtained by means of the classical one-stage method (24.8%). A good correlation was observed among the different methods. However, the levels observed using the Notechis scutatus scutatus venom method were slightly higher as compared to those obtained by means of the other viper venoms and by means of the classical one-stage method. The three viper venoms used seem unable to activate coumarin-induced prothrombin. The levels obtained were in fact, in each instance, definitely lower than those observed immunologically. Methods which employ these viper venoms may be used in the evaluation of prothrombin in coumarin-treated patients.", "contents": "Viper venoms and coumarin-induced prothrombin. A comparison of several one-stage methods employing three different venoms as thromboplastins. Three venoms obtained from three vipers, namely Echis carinatus, Notechis scutatus scutatus and Oxyuranus scutellatus, have been used as thromboplastin in a one-stage assay of coumarin-induced prothrombin. Regardless of the venom used, prothrombin resulted to be low in coumarin-treated patients. The mean values obtained were 27.2, 33.6, and 24.2%, respectively. These values were comparable to those obtained by means of the classical one-stage method (24.8%). A good correlation was observed among the different methods. However, the levels observed using the Notechis scutatus scutatus venom method were slightly higher as compared to those obtained by means of the other viper venoms and by means of the classical one-stage method. The three viper venoms used seem unable to activate coumarin-induced prothrombin. The levels obtained were in fact, in each instance, definitely lower than those observed immunologically. Methods which employ these viper venoms may be used in the evaluation of prothrombin in coumarin-treated patients.", "PMID": 959712}
{"id": "pubmed23n0032_3558", "title": "[Epidemiologic and etiologic factors in cancer of the breast. Catamnestic investigations on 749 patients of the Department of Radiology at Essen (author's transl)].", "content": "Morbidity- and morality statistics of cancer of the breast show a clear increase in almost all countries. The numbers of patients who died increased particularly in countries situated around the north-sea. It appears that unmarried women run a greater risk, as reported by many authors. On further risks we could not confirm that those women with few children predominated. Our results correspond to numbers calculated for abortions. More children increase the danger of breast cancer. These evaluations cannot be separated from the impact of shortened breast-feeding and sociologic factors which also influence the risk in an ill-defined manner. Breast-cancer is doubly to five times as common in female relatives of patients as shown in Anglo-american and our own materials. Age distribution among 742 patients shows, rather like that of some Scandinavian authors, peaks between 45 and 49 and between 56 and 63 years. This again raises the question of a post-menopausal stimulus. In our material there is an average delary of 4 1/2 months between first symptoms and start of treatment. The causes arise both from patients and doctors and, age apart, are responsible for the undue delay. Delay, invasion of lymph nodes and size of tumor establish a correlation between delay and stage of tumor. As to the site of the tomor the left breast was more often involved than the right in our patients. The upper lateral quadrant predominated. To extend the period of survival we shall have to reduce delay and develop further special techniques of treatment.", "contents": "[Epidemiologic and etiologic factors in cancer of the breast. Catamnestic investigations on 749 patients of the Department of Radiology at Essen (author's transl)]. Morbidity- and morality statistics of cancer of the breast show a clear increase in almost all countries. The numbers of patients who died increased particularly in countries situated around the north-sea. It appears that unmarried women run a greater risk, as reported by many authors. On further risks we could not confirm that those women with few children predominated. Our results correspond to numbers calculated for abortions. More children increase the danger of breast cancer. These evaluations cannot be separated from the impact of shortened breast-feeding and sociologic factors which also influence the risk in an ill-defined manner. Breast-cancer is doubly to five times as common in female relatives of patients as shown in Anglo-american and our own materials. Age distribution among 742 patients shows, rather like that of some Scandinavian authors, peaks between 45 and 49 and between 56 and 63 years. This again raises the question of a post-menopausal stimulus. In our material there is an average delary of 4 1/2 months between first symptoms and start of treatment. The causes arise both from patients and doctors and, age apart, are responsible for the undue delay. Delay, invasion of lymph nodes and size of tumor establish a correlation between delay and stage of tumor. As to the site of the tomor the left breast was more often involved than the right in our patients. The upper lateral quadrant predominated. To extend the period of survival we shall have to reduce delay and develop further special techniques of treatment.", "PMID": 959715}
{"id": "pubmed23n0032_3559", "title": "[The place of xerography combined with routine conventional mammography (author's transl)].", "content": "Xeroradiography is a valuable supplement to conventional mammography, especially in cases with dense glandular structure. In our patient material this new procedure was necessary for clarification of findings on conventional mammography in 16% of cases and improved diagnostic yield. Indirect and direct signs of carcinoma were more easely recognized with xeroradiography, since more detail is visualized because of greater latitude and edge contrast enhancement. Moreover the area visualized is considerably greater. The soft tissue structures can be seen to the level of the thoracic wall. Viewing the films is less tiring than reviewing conventional mammography films. A relatively greater exposure latitude and short exposure times are further advantages of this method.", "contents": "[The place of xerography combined with routine conventional mammography (author's transl)]. Xeroradiography is a valuable supplement to conventional mammography, especially in cases with dense glandular structure. In our patient material this new procedure was necessary for clarification of findings on conventional mammography in 16% of cases and improved diagnostic yield. Indirect and direct signs of carcinoma were more easely recognized with xeroradiography, since more detail is visualized because of greater latitude and edge contrast enhancement. Moreover the area visualized is considerably greater. The soft tissue structures can be seen to the level of the thoracic wall. Viewing the films is less tiring than reviewing conventional mammography films. A relatively greater exposure latitude and short exposure times are further advantages of this method.", "PMID": 959716}
{"id": "pubmed23n0032_3560", "title": "[Mammographic observations on the growth of untreated cancer of the breast (author's transl)].", "content": "Report on 21 primary breast cancers in 19 untreated women. The longest follow-up was 1074 days, the shortest 24 days. Doubling time of the tumors was calculated with Collins formula. The necessary assessment of the volume of tumors was calculated with a simple method. Doubling time differs greatly and, in parts, greatly exceeds published data. The radiologic development is surveyed. Collins formula is discussed in its strict interpretation starting from the volume of one tumor cell. Our own results lead to conclusions on the radiologic diagnosis of breast cancer. Usually diagnosis is immediately followed by therapy. Observations on the spontaneous course of, expecially primary, cancer of the breast are therefore scanty. Larger numbers of repeated mammography of the same tumor do not exist. Clinical examinations of growth belong to earlier times, the patients even to past centuries (Bloom 1964). Systematic investigations of this type cannot be defended on ethical grounds. In order to acquire insight into spontaneous growth of this cancer one has to rely on the few cases in whom operation wa5 not done for various reasons Here repeat mammography was possible. Such cases are important in practice since repeat mammography may have to be recommended by the radiologist, our own material will be discussed.", "contents": "[Mammographic observations on the growth of untreated cancer of the breast (author's transl)]. Report on 21 primary breast cancers in 19 untreated women. The longest follow-up was 1074 days, the shortest 24 days. Doubling time of the tumors was calculated with Collins formula. The necessary assessment of the volume of tumors was calculated with a simple method. Doubling time differs greatly and, in parts, greatly exceeds published data. The radiologic development is surveyed. Collins formula is discussed in its strict interpretation starting from the volume of one tumor cell. Our own results lead to conclusions on the radiologic diagnosis of breast cancer. Usually diagnosis is immediately followed by therapy. Observations on the spontaneous course of, expecially primary, cancer of the breast are therefore scanty. Larger numbers of repeated mammography of the same tumor do not exist. Clinical examinations of growth belong to earlier times, the patients even to past centuries (Bloom 1964). Systematic investigations of this type cannot be defended on ethical grounds. In order to acquire insight into spontaneous growth of this cancer one has to rely on the few cases in whom operation wa5 not done for various reasons Here repeat mammography was possible. Such cases are important in practice since repeat mammography may have to be recommended by the radiologist, our own material will be discussed.", "PMID": 959717}
{"id": "pubmed23n0032_3561", "title": "[Cut-density in angiotomography (author's transl)].", "content": "Tests show that the usual definition of cut-density is not applicable to angiotomography. New appropriate data for definition should take account of the relation between vessel and direction of cut and between timing of cut and contrast. These data cannot be obtained by examination in vivo. The concept of cut-density should be abandoned for inear angiotomography and replaced by that of layer of definition.", "contents": "[Cut-density in angiotomography (author's transl)]. Tests show that the usual definition of cut-density is not applicable to angiotomography. New appropriate data for definition should take account of the relation between vessel and direction of cut and between timing of cut and contrast. These data cannot be obtained by examination in vivo. The concept of cut-density should be abandoned for inear angiotomography and replaced by that of layer of definition.", "PMID": 959718}
{"id": "pubmed23n0032_3562", "title": "[Posterior fossa angiomata with slow evolution: clinical aspects (author's transl)].", "content": "The AA. report 9 cases of arteriovenous angiomata in the posterior cranial fossa with chronic evolution: in 8 of these the diagnosis was made before a subarachnoid haemorrhagic episode precipitated the symptomatology. Angiomata in the posterior cranial fossa are detected less often than sopratentorial angiomata, and the possible reasons of this are considered. During the last 11 years in the Neurosurgical Institute of the University of Padua the incidence was 23%, one of the highest reported in literature. The clinical diagnostic criteria of angioma in the posterior cranial fossa with chronic evolution are also discussed. The importance of a fluctuating development of the symptomatology is emphasised with an almost constant association of signs of pyramidal and cerebellar involvement, as well as the differential diagnostic problems with multiple sclerosis. In six of the cases the malformation was so extensive to make it impossible to determine afferences and drainages, so that a surgical operation could not be carried out. This finding seemed rather peculiar, owing to the relatively poor simptomatology, mainly if compared with smaller supratentorial malformations. It appears from the literature that angiomata in the posterior cranial fossa including those found at autopsy are as common as the supratentorial ones; this would suggest that many of these lesions are not diagnosed in life.", "contents": "[Posterior fossa angiomata with slow evolution: clinical aspects (author's transl)]. The AA. report 9 cases of arteriovenous angiomata in the posterior cranial fossa with chronic evolution: in 8 of these the diagnosis was made before a subarachnoid haemorrhagic episode precipitated the symptomatology. Angiomata in the posterior cranial fossa are detected less often than sopratentorial angiomata, and the possible reasons of this are considered. During the last 11 years in the Neurosurgical Institute of the University of Padua the incidence was 23%, one of the highest reported in literature. The clinical diagnostic criteria of angioma in the posterior cranial fossa with chronic evolution are also discussed. The importance of a fluctuating development of the symptomatology is emphasised with an almost constant association of signs of pyramidal and cerebellar involvement, as well as the differential diagnostic problems with multiple sclerosis. In six of the cases the malformation was so extensive to make it impossible to determine afferences and drainages, so that a surgical operation could not be carried out. This finding seemed rather peculiar, owing to the relatively poor simptomatology, mainly if compared with smaller supratentorial malformations. It appears from the literature that angiomata in the posterior cranial fossa including those found at autopsy are as common as the supratentorial ones; this would suggest that many of these lesions are not diagnosed in life.", "PMID": 959713}
{"id": "pubmed23n0032_3563", "title": "[Simple tests to control a tomographic device (author's transl)].", "content": "Simple tests to prove the resolution sharpness and blurring of a tomographic system are described.", "contents": "[Simple tests to control a tomographic device (author's transl)]. Simple tests to prove the resolution sharpness and blurring of a tomographic system are described.", "PMID": 959719}
{"id": "pubmed23n0032_3564", "title": "[A case of extreme gouty arthritis, shown xeroradiographically (author's transl)].", "content": "A case of extreme, obviously lead-induced gouty arthritis is described testing xerography for typical gouty changes. Xerography is less effective in showing differences of density than film-techniques. But highly structures shapes or their sudden abolition will be particularly clear owing to the effect of contour reinforcement. Defects of bone induced by tophi and new formations can be identified with certainly.", "contents": "[A case of extreme gouty arthritis, shown xeroradiographically (author's transl)]. A case of extreme, obviously lead-induced gouty arthritis is described testing xerography for typical gouty changes. Xerography is less effective in showing differences of density than film-techniques. But highly structures shapes or their sudden abolition will be particularly clear owing to the effect of contour reinforcement. Defects of bone induced by tophi and new formations can be identified with certainly.", "PMID": 959720}
{"id": "pubmed23n0032_3565", "title": "[Problems in the diagnosis of pheochromocytoma (author's transl)].", "content": "Reference is made to various possibilities of examination in diagnosis and localisation of pheochromocytoma demonstrated by several cases. The best radiological examination is angiography using Seldinger method, which by means of skilled technique, good contrast media and an optimal treatment with receptor-blocking medicaments will guarantee excellent x-rays for clear tumor localisation without real complications.", "contents": "[Problems in the diagnosis of pheochromocytoma (author's transl)]. Reference is made to various possibilities of examination in diagnosis and localisation of pheochromocytoma demonstrated by several cases. The best radiological examination is angiography using Seldinger method, which by means of skilled technique, good contrast media and an optimal treatment with receptor-blocking medicaments will guarantee excellent x-rays for clear tumor localisation without real complications.", "PMID": 959721}
{"id": "pubmed23n0032_3566", "title": "[Angiographic diagnosis of a carcinoma of the renal pelvis due to thorotrast (author's transl)].", "content": "Angiographic and histologic findings of a carcinoma of the renal pelvis of low vascularity. Angiography succeeds in demonstrating the tumor, the site of its origin and spatial relationship to the escape of contrast-medium.", "contents": "[Angiographic diagnosis of a carcinoma of the renal pelvis due to thorotrast (author's transl)]. Angiographic and histologic findings of a carcinoma of the renal pelvis of low vascularity. Angiography succeeds in demonstrating the tumor, the site of its origin and spatial relationship to the escape of contrast-medium.", "PMID": 959722}
{"id": "pubmed23n0032_3567", "title": "[A new contrast-medium (author's transl)].", "content": "Infusion urograms were carried out on 2 groups of 100 in-patients each. The first group had injections of Conray FL 36, the second of Conray FL. Contrast in the early phase after 7 minutes was better with Conray FL 36 than with Conray FL. At later stages there was no clear difference.", "contents": "[A new contrast-medium (author's transl)]. Infusion urograms were carried out on 2 groups of 100 in-patients each. The first group had injections of Conray FL 36, the second of Conray FL. Contrast in the early phase after 7 minutes was better with Conray FL 36 than with Conray FL. At later stages there was no clear difference.", "PMID": 959723}
{"id": "pubmed23n0032_3568", "title": "[Lymphography in testicular tumors (author's transl)].", "content": "Results of lymphography in 101 cases of testicular tumor are described. Secondaries could be shown in 43% of seminomas and 46% of teratomas. Examples show the criteria of lymphographic diagnosis of secondaries. Lymphography permits a) more exact classification of stages b) better planning and control of treatment and c) prognosis.", "contents": "[Lymphography in testicular tumors (author's transl)]. Results of lymphography in 101 cases of testicular tumor are described. Secondaries could be shown in 43% of seminomas and 46% of teratomas. Examples show the criteria of lymphographic diagnosis of secondaries. Lymphography permits a) more exact classification of stages b) better planning and control of treatment and c) prognosis.", "PMID": 959724}
{"id": "pubmed23n0032_3569", "title": "[Protection of gonads in radiography of the pelvis (author's transl)].", "content": "The effectiveness of a new device for the protection of gonads has been tested by measuring doses on the water-phantom and on patients. The lead protector is applied below the light-soorce and protects the gonads in pelvic radiography. The reduction of dosage corresponds to the usual lead-protectors. It should be particularly useful in Orthopedics, since it is simple and hygienic.", "contents": "[Protection of gonads in radiography of the pelvis (author's transl)]. The effectiveness of a new device for the protection of gonads has been tested by measuring doses on the water-phantom and on patients. The lead protector is applied below the light-soorce and protects the gonads in pelvic radiography. The reduction of dosage corresponds to the usual lead-protectors. It should be particularly useful in Orthopedics, since it is simple and hygienic.", "PMID": 959725}
{"id": "pubmed23n0032_3570", "title": "[Xerographic diagnosis of lesions of the cruciate ligaments of the knee-joint (author's transl)].", "content": "First experience with xerography of the cruciate ligaments and their lesions in compared with the usual radiographic techniques. Both cruciate ligaments can be shown more often and with sharper contours by using double contrast-arthrography in xeroradiograms. The specific pictorial characteristics of xeroradiography are discussed.", "contents": "[Xerographic diagnosis of lesions of the cruciate ligaments of the knee-joint (author's transl)]. First experience with xerography of the cruciate ligaments and their lesions in compared with the usual radiographic techniques. Both cruciate ligaments can be shown more often and with sharper contours by using double contrast-arthrography in xeroradiograms. The specific pictorial characteristics of xeroradiography are discussed.", "PMID": 959726}
{"id": "pubmed23n0032_3571", "title": "[Pyloric stenosis - a complication of cholelithiasis. (Case of gallstone perforation into the duodenal bulb)].", "content": "Pressure-necrosis followed by stone perforation is ralatively rare among the complications of cholelithiasis. There is always a danger of a stenosis in the gastro-intestinal tract. Report of a case of perforation of a gallstone into the duodenal bulb producing few signs but the clinical picture of pyloric stenosis. Symptoms, signs and differential diagnosis are discussed on the basis of the literature.", "contents": "[Pyloric stenosis - a complication of cholelithiasis. (Case of gallstone perforation into the duodenal bulb)]. Pressure-necrosis followed by stone perforation is ralatively rare among the complications of cholelithiasis. There is always a danger of a stenosis in the gastro-intestinal tract. Report of a case of perforation of a gallstone into the duodenal bulb producing few signs but the clinical picture of pyloric stenosis. Symptoms, signs and differential diagnosis are discussed on the basis of the literature.", "PMID": 959727}
{"id": "pubmed23n0032_3572", "title": "[Concentration of contrast medium in xerophlebography (author's transl)].", "content": "We examine with phlebography usually a damaged vascular system. Radio-contrastmedia damage vascular endothelium. If we dilute them we may minimize the damaging effect. But when using the usual film-foliae combination, diagnostic results diminish the more we dilute. Results of 31 comparative investigations with film and xerphlebography show that dilution of contrast medium to 50 or even 25% will still produce phlebograms sufficient for evaluation with xerophlebography. It also has the advantage of a large picture: soft parts, veins and bony structures are visible of the same time. At present the method (chance of screening, gaps) renders routine use not advisable.", "contents": "[Concentration of contrast medium in xerophlebography (author's transl)]. We examine with phlebography usually a damaged vascular system. Radio-contrastmedia damage vascular endothelium. If we dilute them we may minimize the damaging effect. But when using the usual film-foliae combination, diagnostic results diminish the more we dilute. Results of 31 comparative investigations with film and xerphlebography show that dilution of contrast medium to 50 or even 25% will still produce phlebograms sufficient for evaluation with xerophlebography. It also has the advantage of a large picture: soft parts, veins and bony structures are visible of the same time. At present the method (chance of screening, gaps) renders routine use not advisable.", "PMID": 959728}
{"id": "pubmed23n0032_3573", "title": "[Phlebography of the lower limb (author's transl)].", "content": "Description of the techniques of ascending phlebography and retrograde pressure phlebography of the leg and of means of showing insufficient perforating veins with selective percutaneous or intra-operative injection of contrast-media. Misinterpretation of the deep veins owing to muscular contraction for insufficiently continuous filling is explained. Phlebographic diagnosis of recent phlebothromboses, taking into accout isotope diagnosis, is particularly important for the recognition of post-traumatic so-called masked thromboses. The effectiveness of phlebography for the clarification of neurosegmental angiomatoses is discussed.", "contents": "[Phlebography of the lower limb (author's transl)]. Description of the techniques of ascending phlebography and retrograde pressure phlebography of the leg and of means of showing insufficient perforating veins with selective percutaneous or intra-operative injection of contrast-media. Misinterpretation of the deep veins owing to muscular contraction for insufficiently continuous filling is explained. Phlebographic diagnosis of recent phlebothromboses, taking into accout isotope diagnosis, is particularly important for the recognition of post-traumatic so-called masked thromboses. The effectiveness of phlebography for the clarification of neurosegmental angiomatoses is discussed.", "PMID": 959729}
{"id": "pubmed23n0032_3574", "title": "[Isotope phlebography (author's transl)].", "content": "Various techniques for showing venous function are recognized. The value of isotope phlebography is shown here on 39 unselected cases in whom contrast filling was also carried out. Rapid injection of 2 m Ci TC 99m into a dorsal vein of the foot produced isotope phlebograms with a Dyna camera 2 C.", "contents": "[Isotope phlebography (author's transl)]. Various techniques for showing venous function are recognized. The value of isotope phlebography is shown here on 39 unselected cases in whom contrast filling was also carried out. Rapid injection of 2 m Ci TC 99m into a dorsal vein of the foot produced isotope phlebograms with a Dyna camera 2 C.", "PMID": 959730}
{"id": "pubmed23n0032_3575", "title": "[Radioiodine-fibrinogen test in the early diagnosis of venous thrombosis in the leg (author's transl)].", "content": "As long as there is no satisfactory prophylaxis for thrombosis, the radioiodine-fibrinogen test is a good supplement for the supervision of patients with a \"high thrombosis risk\" since the clinical symptomatology is not specific enough and phlebography cannot be used to extensively. The risk of hepatitis can be reduced when the selection of spenders is large enough. As a radionuclide, 125iodine does not appear to be any less effective than 131iodine. The postoperative injection is preferred to the preoperative injection.", "contents": "[Radioiodine-fibrinogen test in the early diagnosis of venous thrombosis in the leg (author's transl)]. As long as there is no satisfactory prophylaxis for thrombosis, the radioiodine-fibrinogen test is a good supplement for the supervision of patients with a \"high thrombosis risk\" since the clinical symptomatology is not specific enough and phlebography cannot be used to extensively. The risk of hepatitis can be reduced when the selection of spenders is large enough. As a radionuclide, 125iodine does not appear to be any less effective than 131iodine. The postoperative injection is preferred to the preoperative injection.", "PMID": 959731}
{"id": "pubmed23n0032_3576", "title": "[Technique and value of discography for the cervical syndrome (author's transl)].", "content": "Discography produces relatively many false pasitive findings. Their number, about 28% in our material, may be reduced decisively to below 10% when clinical, electro-physiologic and myelographic findings are considered together. It can be further reduced when results are judged in groups of varying value. Findings of high value are: 1) entry of the contrast fluid from the disc behind the posterior longitudinal ligament. 2) Exact reproduction of spontaneous pain during injection 3) The extension of a contrast band along the roots on the side and level of clinical findings. With such strict evaluation discographic findings are correct in 88%. In additon discography may be in certain cases the only method to elucidate a syndrome which clinically and in the myelogram permits various interpretations. Discography should always preceded operation. On the basis of 368 discographies with control of the diagnosis at operation its value is explained.", "contents": "[Technique and value of discography for the cervical syndrome (author's transl)]. Discography produces relatively many false pasitive findings. Their number, about 28% in our material, may be reduced decisively to below 10% when clinical, electro-physiologic and myelographic findings are considered together. It can be further reduced when results are judged in groups of varying value. Findings of high value are: 1) entry of the contrast fluid from the disc behind the posterior longitudinal ligament. 2) Exact reproduction of spontaneous pain during injection 3) The extension of a contrast band along the roots on the side and level of clinical findings. With such strict evaluation discographic findings are correct in 88%. In additon discography may be in certain cases the only method to elucidate a syndrome which clinically and in the myelogram permits various interpretations. Discography should always preceded operation. On the basis of 368 discographies with control of the diagnosis at operation its value is explained.", "PMID": 959732}
{"id": "pubmed23n0032_3577", "title": "[Comparison between myelography and discography for lumbar prolapsed intervertebral discs (author's transl)].", "content": "For the diagnosis of lumbar P.I.D. discography is much superior to myelography. The risks of both techniques are the same, but the side-effects of discography are clearly less. Higher lesions remain a problem if they do not show up neurologically (s. the prolapsed disc at L 2/3). Here segmental blocking, posterior gaping of the intervertebral space and displacement of the dural sac show clearly the posterior prolapse. In the standard discogram of the lower 3 discs it would have been missed. In spite of this our present experience with lumbar discography is so positive, that we are about to test a long term series, whether and to which extend discography could replace myelography in cases where there is no hint at a space-occupying lesion, other than a disc or whether the order of these investigations should be reversed.", "contents": "[Comparison between myelography and discography for lumbar prolapsed intervertebral discs (author's transl)]. For the diagnosis of lumbar P.I.D. discography is much superior to myelography. The risks of both techniques are the same, but the side-effects of discography are clearly less. Higher lesions remain a problem if they do not show up neurologically (s. the prolapsed disc at L 2/3). Here segmental blocking, posterior gaping of the intervertebral space and displacement of the dural sac show clearly the posterior prolapse. In the standard discogram of the lower 3 discs it would have been missed. In spite of this our present experience with lumbar discography is so positive, that we are about to test a long term series, whether and to which extend discography could replace myelography in cases where there is no hint at a space-occupying lesion, other than a disc or whether the order of these investigations should be reversed.", "PMID": 959733}
{"id": "pubmed23n0032_3578", "title": "[Microlithiasis alveolaris pulmonum. (author's transl)].", "content": "A report was made of a long term observation of microlethiasis alveolaris pulmonum in a female patient who died at the age of 53. The rare, etiologically unclear disease is after a long course , always fatal due to right heart failure. This case was unique in that small spotty pulmonary lesions were found radiologically. They presented, a pathognomonic, unmistakable picture for a differential diagnosis of silicosis.", "contents": "[Microlithiasis alveolaris pulmonum. (author's transl)]. A report was made of a long term observation of microlethiasis alveolaris pulmonum in a female patient who died at the age of 53. The rare, etiologically unclear disease is after a long course , always fatal due to right heart failure. This case was unique in that small spotty pulmonary lesions were found radiologically. They presented, a pathognomonic, unmistakable picture for a differential diagnosis of silicosis.", "PMID": 959734}
{"id": "pubmed23n0032_3579", "title": "[Calcifications in mediastinal lymphoma after radiation therapy of Hodgkin's disease. (author's transl)].", "content": "One case of calcifications in mediastihal lymphoma after radiation therapy of Hodgkin's disease is reported. The incidence of these calcifications is remarkably low. They are mostly localized in the anterior mediastinum showing a characteristical pattern which is initially stippled, later confluent and coral-shaped. An open interval after radiation therapy is typical for this phenomenon. The cause of the calcifications is discussed.", "contents": "[Calcifications in mediastinal lymphoma after radiation therapy of Hodgkin's disease. (author's transl)]. One case of calcifications in mediastihal lymphoma after radiation therapy of Hodgkin's disease is reported. The incidence of these calcifications is remarkably low. They are mostly localized in the anterior mediastinum showing a characteristical pattern which is initially stippled, later confluent and coral-shaped. An open interval after radiation therapy is typical for this phenomenon. The cause of the calcifications is discussed.", "PMID": 959735}
{"id": "pubmed23n0032_3580", "title": "[Diagnosis and differential diagnosis of the terminal esophagus. (author's transl)].", "content": "On hand of a few selected cases diagnostic and differential diagnostic problems of the cardia are discussed. Radiological and endoscopical values are presented.", "contents": "[Diagnosis and differential diagnosis of the terminal esophagus. (author's transl)]. On hand of a few selected cases diagnostic and differential diagnostic problems of the cardia are discussed. Radiological and endoscopical values are presented.", "PMID": 959736}
{"id": "pubmed23n0032_3581", "title": "[The cholecystogram as a prerequisite of the chemical litholysis from the view of the roentgenologist. (author's transl)].", "content": "The preoperative cholecystography and postoperative radiography of gallstones were compared in 68 patients. Calcified structures were detected in 32% of the stones which were free of calcium in the native roentgenograms. The possibility is stressed that the calciumocontent of cholecystographic transparent stones may be responsible for the failure of CDCA-therapy. 52 of the 68 patients additionally received a fat meal. A contraction of the gallbladder was not observed in 25% of these cases. From the missing contraction of the gallbladder should not be concluded that a CDCA-therapy is contraindicated. A functional analysis with a synthetic derivate of cholecystokinin is recommended.", "contents": "[The cholecystogram as a prerequisite of the chemical litholysis from the view of the roentgenologist. (author's transl)]. The preoperative cholecystography and postoperative radiography of gallstones were compared in 68 patients. Calcified structures were detected in 32% of the stones which were free of calcium in the native roentgenograms. The possibility is stressed that the calciumocontent of cholecystographic transparent stones may be responsible for the failure of CDCA-therapy. 52 of the 68 patients additionally received a fat meal. A contraction of the gallbladder was not observed in 25% of these cases. From the missing contraction of the gallbladder should not be concluded that a CDCA-therapy is contraindicated. A functional analysis with a synthetic derivate of cholecystokinin is recommended.", "PMID": 959737}
{"id": "pubmed23n0032_3582", "title": "[Suggestions for a routine methode. (author's transl)].", "content": "The advantages of the Shirakabe double-contrast method are well known. Nevertheless, the time involved to conduct the technique and the methods eliminate it from use as a standard examination technique. It is desirable to look for a practicable modification which can be used routinely in the medical practice. The above report deals with this problem.", "contents": "[Suggestions for a routine methode. (author's transl)]. The advantages of the Shirakabe double-contrast method are well known. Nevertheless, the time involved to conduct the technique and the methods eliminate it from use as a standard examination technique. It is desirable to look for a practicable modification which can be used routinely in the medical practice. The above report deals with this problem.", "PMID": 959738}
{"id": "pubmed23n0032_3583", "title": "[Patient identification (PnI) in oversized radiographs. (author's transl)].", "content": "In comparison with the \"Skribor strips\", the PnI system offers a high degree of certainty for the correct classification of patient data in regard to the X-ray and the documentation of the correct time. The existing difficiencies can be compensated for by further technical developments. The screen developed for this system requires a lower dose than the FZ secreen in order to obtain the same degree of detail.", "contents": "[Patient identification (PnI) in oversized radiographs. (author's transl)]. In comparison with the \"Skribor strips\", the PnI system offers a high degree of certainty for the correct classification of patient data in regard to the X-ray and the documentation of the correct time. The existing difficiencies can be compensated for by further technical developments. The screen developed for this system requires a lower dose than the FZ secreen in order to obtain the same degree of detail.", "PMID": 959739}
{"id": "pubmed23n0032_3584", "title": "A sensitive radioimmunoassay of parathyroid hormone in human serum using a specific extraction procedure.", "content": "A radioimmunoassay of human parathroid hormone (PTH) is described. PTH was extracted from serum by adsorption to and elution from microfine silica, causing a 3.2-fold greater hormone concentration in extract than in serum. The extraction procedure eliminated from the assay unspecific interference caused by serum factors. The detection limit was 10 pg bovine PTH. The concentration of PTH was measurable in serum from 95% of normal subjects (mean, 68 pg/ml; SD., 18), was undetectable in sera from hypoparathyroid patients, and elevated in 96% of sera from patients with primary hyperparathyroidism. The presented data suggest that the assay mainly measures PTH(1-84).", "contents": "A sensitive radioimmunoassay of parathyroid hormone in human serum using a specific extraction procedure. A radioimmunoassay of human parathroid hormone (PTH) is described. PTH was extracted from serum by adsorption to and elution from microfine silica, causing a 3.2-fold greater hormone concentration in extract than in serum. The extraction procedure eliminated from the assay unspecific interference caused by serum factors. The detection limit was 10 pg bovine PTH. The concentration of PTH was measurable in serum from 95% of normal subjects (mean, 68 pg/ml; SD., 18), was undetectable in sera from hypoparathyroid patients, and elevated in 96% of sera from patients with primary hyperparathyroidism. The presented data suggest that the assay mainly measures PTH(1-84).", "PMID": 959749}
{"id": "pubmed23n0032_3585", "title": "Radioimmunoassay of human calcitonin in serum and tissue from healthy individuals and patients with medullary carcinoma of the thyroid gland.", "content": "A specific radioimmunological method for measurement of immunoreactive calcitonin (iCT) in human serum and tissue is decribed. Of healthy individuals of both sexes, 85% had measurable iCT in serum (mean, 0.23 ng/ml). Of  29 patients who had received treatment for medullary carcinoma of the thyroid gland (MCT), 19 had increased serum iCT (0.60 ng/ml to 205 ng/ml). Elevated serum iCT was also found preoperatively in 2 MCT patients. Eleven of the patients with abnormal elevations of serum iCT were alive 4 to 13 years after the operation. Concentration of iCT in extracts from MCT varied from 0.5 to 540 ng/ml wet weight. The diagnostic value of this method and its importance for pre- and post-operative evaluation of these patients are improved by the use of selective venous catheterization in basal state and during stimulation of CT secretion.", "contents": "Radioimmunoassay of human calcitonin in serum and tissue from healthy individuals and patients with medullary carcinoma of the thyroid gland. A specific radioimmunological method for measurement of immunoreactive calcitonin (iCT) in human serum and tissue is decribed. Of healthy individuals of both sexes, 85% had measurable iCT in serum (mean, 0.23 ng/ml). Of  29 patients who had received treatment for medullary carcinoma of the thyroid gland (MCT), 19 had increased serum iCT (0.60 ng/ml to 205 ng/ml). Elevated serum iCT was also found preoperatively in 2 MCT patients. Eleven of the patients with abnormal elevations of serum iCT were alive 4 to 13 years after the operation. Concentration of iCT in extracts from MCT varied from 0.5 to 540 ng/ml wet weight. The diagnostic value of this method and its importance for pre- and post-operative evaluation of these patients are improved by the use of selective venous catheterization in basal state and during stimulation of CT secretion.", "PMID": 959750}
{"id": "pubmed23n0032_3586", "title": "Evaluation of radioimmunological methods for assay of plasma and urinary aldosterone.", "content": "Two radioimmunological methods for assay of plasma and urinary aldosterone were carefully evaluated. In the plasma method a radioimmunoassay is preceded by chromatography on a Sephadex LH-20 column. The method for urine includes a preextraction, hydrolysis of the acid-labile conjugates of aldosterone, and a radioimmunoassay. Both methods fulfill the criteria of reliability and are suitable for both routine and demanding research assays. The plasma method, using columns of double length, is also applicable to analysis of aldosterone in plasma of newborn children, and pregnant females and in cord plasma. The concentration of plasma aldosterone in healthy subjects on an ad lib salt diet was 162 +/- 93 (S.D.) pmol/1 in the supine position and 312 +/- 217 (S.D.) pmol/1 upright. The urinary excretion of aldosterone in healthy subjects was 28.3 +/- 16.7 (S.D.) nmol/24 h.", "contents": "Evaluation of radioimmunological methods for assay of plasma and urinary aldosterone. Two radioimmunological methods for assay of plasma and urinary aldosterone were carefully evaluated. In the plasma method a radioimmunoassay is preceded by chromatography on a Sephadex LH-20 column. The method for urine includes a preextraction, hydrolysis of the acid-labile conjugates of aldosterone, and a radioimmunoassay. Both methods fulfill the criteria of reliability and are suitable for both routine and demanding research assays. The plasma method, using columns of double length, is also applicable to analysis of aldosterone in plasma of newborn children, and pregnant females and in cord plasma. The concentration of plasma aldosterone in healthy subjects on an ad lib salt diet was 162 +/- 93 (S.D.) pmol/1 in the supine position and 312 +/- 217 (S.D.) pmol/1 upright. The urinary excretion of aldosterone in healthy subjects was 28.3 +/- 16.7 (S.D.) nmol/24 h.", "PMID": 959751}
{"id": "pubmed23n0032_3587", "title": "Gluconeogenesis in human liver tissue. An in vitro method for evaluation of glyconeogenesis in man.", "content": "Human liver tissue was obtained as surgical biopsies in 29 subjects operated on for uncomplicated gallstone disease. Liver slices were incubated in Krebs-Ringer bicarbonate buffer solution, pH 7.4, with 17 l-amino acids, lactate, glycerol, and glucose at various concentrations. The incorporation rate of alanine, lactate, and glycerol into glucose, glycogen, and CO2 was determined by use of 14C-labeled precursors. The gluconeogenetic rate of all substrates was increased 10-35 times by increasing precursor concentration in the medium. Insulin at a physiological concentration (300 mU/l) and dexamethasone  (0.001 mmol/l) had slight but significant effects on the incorporation rate of alanine into glucose and glycogen, respectively. Glucagon had no effect. Glucose in the incubation medium did not influence the incorporation rate of precursors into glucose, glycogen, or CO2, suggesting that glucose was not of importance for the regulation of the gluconeogenesis. The gluconeogenetic rate of a precursor was not dependent on or influenced by the presence of other precursors. The gluconeogenesis in human liver slices at physiological concentrations of precursors was 5-20% of the maximal rate reported for the rat liver. When the precursor concentration in the medium was increased, the gluconeogenetic rate increased to values close to those reported for the rat liver in vitro and for man in vivo. This in vitro preparation of human liver seems to be valid for evaluation of gluconeogenesis in man.", "contents": "Gluconeogenesis in human liver tissue. An in vitro method for evaluation of glyconeogenesis in man. Human liver tissue was obtained as surgical biopsies in 29 subjects operated on for uncomplicated gallstone disease. Liver slices were incubated in Krebs-Ringer bicarbonate buffer solution, pH 7.4, with 17 l-amino acids, lactate, glycerol, and glucose at various concentrations. The incorporation rate of alanine, lactate, and glycerol into glucose, glycogen, and CO2 was determined by use of 14C-labeled precursors. The gluconeogenetic rate of all substrates was increased 10-35 times by increasing precursor concentration in the medium. Insulin at a physiological concentration (300 mU/l) and dexamethasone  (0.001 mmol/l) had slight but significant effects on the incorporation rate of alanine into glucose and glycogen, respectively. Glucagon had no effect. Glucose in the incubation medium did not influence the incorporation rate of precursors into glucose, glycogen, or CO2, suggesting that glucose was not of importance for the regulation of the gluconeogenesis. The gluconeogenetic rate of a precursor was not dependent on or influenced by the presence of other precursors. The gluconeogenesis in human liver slices at physiological concentrations of precursors was 5-20% of the maximal rate reported for the rat liver. When the precursor concentration in the medium was increased, the gluconeogenetic rate increased to values close to those reported for the rat liver in vitro and for man in vivo. This in vitro preparation of human liver seems to be valid for evaluation of gluconeogenesis in man.", "PMID": 959752}
{"id": "pubmed23n0032_3588", "title": "Gluconeogenesis in infancy and childhood. I. A method for the study of the in vivo gluconeogenesis from alanine and glycerol.", "content": "The in vivo gluconeogenesis from alanine and glycerol in infants and children was studied by an isotope method, using 14C-labeled substates with subsequent separation of the radioactive compounds by thin-layer chromatography. Seven patients, aged 2 months to 2 years 11 months, with normal carbohydrate metabolism were studied. Trace amounts of [14C]alanine were injected intravenously in four fasting patients. The 14C moved quickly from alanine to lactate, with a peak activity in lactate obtained before 5 min. From 10 min on, the label disappeared rapidly from both. An equilibrium was established between alanine and lactate, displaced towards lactate. The peak activity in glucose was reached in 10-20 min, amounting to 10% of total injected activity. In one patient, who was also studied after a meal, the disappearance rate of alanine was reduced by 50%. Despite this reduction the appearance of label in lactate was increased, whereas the amount of label in glucose was much reduced. [14C]glycerol was injected intravenously in three fasting patients. In one patient, who received only a tracer dose of glycerol, 5 times more 14C appeared in glucose than in the patients studied with [14C]alanine. In two patients receiving a glycerol load together with the [14C]glycerol, the disappearance rate of glycerol was markedly reduced, as was the conversion of carbon to glucose and lactate.", "contents": "Gluconeogenesis in infancy and childhood. I. A method for the study of the in vivo gluconeogenesis from alanine and glycerol. The in vivo gluconeogenesis from alanine and glycerol in infants and children was studied by an isotope method, using 14C-labeled substates with subsequent separation of the radioactive compounds by thin-layer chromatography. Seven patients, aged 2 months to 2 years 11 months, with normal carbohydrate metabolism were studied. Trace amounts of [14C]alanine were injected intravenously in four fasting patients. The 14C moved quickly from alanine to lactate, with a peak activity in lactate obtained before 5 min. From 10 min on, the label disappeared rapidly from both. An equilibrium was established between alanine and lactate, displaced towards lactate. The peak activity in glucose was reached in 10-20 min, amounting to 10% of total injected activity. In one patient, who was also studied after a meal, the disappearance rate of alanine was reduced by 50%. Despite this reduction the appearance of label in lactate was increased, whereas the amount of label in glucose was much reduced. [14C]glycerol was injected intravenously in three fasting patients. In one patient, who received only a tracer dose of glycerol, 5 times more 14C appeared in glucose than in the patients studied with [14C]alanine. In two patients receiving a glycerol load together with the [14C]glycerol, the disappearance rate of glycerol was markedly reduced, as was the conversion of carbon to glucose and lactate.", "PMID": 959753}
{"id": "pubmed23n0032_3589", "title": "Effect of increased venous pressure on the hydrostatic and colloid osmotic pressure in subcutaneous interstitial fluid in rats: edema-preventing mechanisms.", "content": "The purpose of the present experiments was to study the effect of a rise in local venous pressure pressure (Pv) on interstitial fluid hydrostatic (Pi) and colloid osmotic pressure (COPi) in rats. The Pv of the hind limb was increased by ligating the iliac veins and the inferior caval vein. Interstitial fluid for protein and COP determination was sampled from subcutaneous tissue of the hind limbs by a wick technique, and the Pi was measured by a modified Scholander technique. When the Pv was increased from 3 to 12 mm Hg, the COPi fell from 9 to 4 mm Hg. The Pi averaged -1 mm Hg under control conditions and was unchanged when the Pv was elevated to 12 mm Hg. No edema was detected. Higher rises in venous pressures resulted in further but less pronounced falls in interstitial fluid COP. Edema developed and the Pi increased to +1 to 2mm Hg. The results show that the fall in COPi is an important edema-preventing factor during increased venous pressure, whereas a rise in Pi only seems to oppose further increase of an already existing edema.", "contents": "Effect of increased venous pressure on the hydrostatic and colloid osmotic pressure in subcutaneous interstitial fluid in rats: edema-preventing mechanisms. The purpose of the present experiments was to study the effect of a rise in local venous pressure pressure (Pv) on interstitial fluid hydrostatic (Pi) and colloid osmotic pressure (COPi) in rats. The Pv of the hind limb was increased by ligating the iliac veins and the inferior caval vein. Interstitial fluid for protein and COP determination was sampled from subcutaneous tissue of the hind limbs by a wick technique, and the Pi was measured by a modified Scholander technique. When the Pv was increased from 3 to 12 mm Hg, the COPi fell from 9 to 4 mm Hg. The Pi averaged -1 mm Hg under control conditions and was unchanged when the Pv was elevated to 12 mm Hg. No edema was detected. Higher rises in venous pressures resulted in further but less pronounced falls in interstitial fluid COP. Edema developed and the Pi increased to +1 to 2mm Hg. The results show that the fall in COPi is an important edema-preventing factor during increased venous pressure, whereas a rise in Pi only seems to oppose further increase of an already existing edema.", "PMID": 959754}
{"id": "pubmed23n0032_3590", "title": "Inhibition of meal-stimulated gastrin secretion after oral glucose.", "content": "Serum gastrin concentration was measured in six male subjects before and after a beef meal, with and without 100 g of oral glucose being given 30 min previously. There was a pronounced inhibition of beef-stimulated gastrin secretion after the oral glucose load.", "contents": "Inhibition of meal-stimulated gastrin secretion after oral glucose. Serum gastrin concentration was measured in six male subjects before and after a beef meal, with and without 100 g of oral glucose being given 30 min previously. There was a pronounced inhibition of beef-stimulated gastrin secretion after the oral glucose load.", "PMID": 959755}
{"id": "pubmed23n0032_3591", "title": "Progression of nephropathy in long-term diabetics with proteinuria and effect of initial anti-hypertensive treatment.", "content": "The rate of progression of nephropathy was studied in 6 young male diabetics with intermittent proteinuria (Albustix) and in 10 young male diabetics with constant proteinuria by measuring glomerular filtration rate (GFR), renal plasma flow (RPF), and urinary albumin excretion by exact techniques. Albumin excretion was elevated in both the recumbent and the erect position in patients with intermittent proteinuria. GFR and RPF were at the same level as in diabetics without proteinuria, and no deterioration in renal function was noted during a mean control period of 32 months. In the patients with constant proteinuria the fall rate during a mean period of  33.6 months for GFR and RPF was 0.91 ml/min/month +/- 0.68 (S.D.) and 4.38 ml/min/month +/- 3.23 (S.D.) respectively. Initial fall rate in GFR correlated well with long-term fall rate, both of which were studied in 7 patients. In the same patients there was a positive correlation between the fall rate in GFR and diastolic blood pressure as well as albumin clearance. In 8 patients with constant proteinuria and mean blood pressure of 159/101 mmHg, antihypertensive treatment was started with propranolol alone or combined with hydralazine and furosemide. During a treatment period of 47 days blood pressure was reduced to 143/93 mmHg, and in the same period urinary albumin excretion was reduced significantly from a mean value of 3547 mug/min to 2414 mug/min (P less than 0.01). Further control studies will clarify whether end-stage of renal insufficiency will be postponed by antihypertensive treatment.", "contents": "Progression of nephropathy in long-term diabetics with proteinuria and effect of initial anti-hypertensive treatment. The rate of progression of nephropathy was studied in 6 young male diabetics with intermittent proteinuria (Albustix) and in 10 young male diabetics with constant proteinuria by measuring glomerular filtration rate (GFR), renal plasma flow (RPF), and urinary albumin excretion by exact techniques. Albumin excretion was elevated in both the recumbent and the erect position in patients with intermittent proteinuria. GFR and RPF were at the same level as in diabetics without proteinuria, and no deterioration in renal function was noted during a mean control period of 32 months. In the patients with constant proteinuria the fall rate during a mean period of  33.6 months for GFR and RPF was 0.91 ml/min/month +/- 0.68 (S.D.) and 4.38 ml/min/month +/- 3.23 (S.D.) respectively. Initial fall rate in GFR correlated well with long-term fall rate, both of which were studied in 7 patients. In the same patients there was a positive correlation between the fall rate in GFR and diastolic blood pressure as well as albumin clearance. In 8 patients with constant proteinuria and mean blood pressure of 159/101 mmHg, antihypertensive treatment was started with propranolol alone or combined with hydralazine and furosemide. During a treatment period of 47 days blood pressure was reduced to 143/93 mmHg, and in the same period urinary albumin excretion was reduced significantly from a mean value of 3547 mug/min to 2414 mug/min (P less than 0.01). Further control studies will clarify whether end-stage of renal insufficiency will be postponed by antihypertensive treatment.", "PMID": 959756}
{"id": "pubmed23n0032_3592", "title": "An individual nomogram for determination of glomerular filtration rate from plasma creatinine.", "content": "From simultaneously determined values of the glomerular filtration rate and the concentration of creatinine in plasma an individual nomogram can be constructed from two scales. From this nomogram the actual glomerular filtration rate of the patient can later be read out solely from a new concentration of plasma creatine.", "contents": "An individual nomogram for determination of glomerular filtration rate from plasma creatinine. From simultaneously determined values of the glomerular filtration rate and the concentration of creatinine in plasma an individual nomogram can be constructed from two scales. From this nomogram the actual glomerular filtration rate of the patient can later be read out solely from a new concentration of plasma creatine.", "PMID": 959757}
{"id": "pubmed23n0032_3593", "title": "Micromodification of the Phadebas amylase test.", "content": "A micromodification of the original Phadebas Amylase Test has been adapted for the LKB Calculating Absorptiometer. This test requires only 15 mul of serum and reduces the amount of reagent used by a factor of ten. The results of this semiautomatic micromethod have a coefficient of variation of about 4% and correlate well (r = 0.996) with the method as originally described.", "contents": "Micromodification of the Phadebas amylase test. A micromodification of the original Phadebas Amylase Test has been adapted for the LKB Calculating Absorptiometer. This test requires only 15 mul of serum and reduces the amount of reagent used by a factor of ten. The results of this semiautomatic micromethod have a coefficient of variation of about 4% and correlate well (r = 0.996) with the method as originally described.", "PMID": 959758}
{"id": "pubmed23n0032_3594", "title": "Relationships between gastric acid secretion and recurrent duodenal ulcer after selective vagotomy and pyloroplasty in men.", "content": "Relations between gastric acid secretion measurements and recurrence of duodenal ulcer within 1 to 4 years after selective vagotomy and pyloroplasty were evaluated in a series of 117 men. The discriminatory ability of spontaneous, histamine- and insulin-activated acid secretion measurements was significant and similar to that after truncal vagotomy and drainage. The measurements were of no practical value for the diagnosis of recurrent duodenal ulcer after vagotomy, but they provided a rationale for selective surgery in patients with duodenal ulcer and patients with recurrent duodenal ulcer after vagotomy.", "contents": "Relationships between gastric acid secretion and recurrent duodenal ulcer after selective vagotomy and pyloroplasty in men. Relations between gastric acid secretion measurements and recurrence of duodenal ulcer within 1 to 4 years after selective vagotomy and pyloroplasty were evaluated in a series of 117 men. The discriminatory ability of spontaneous, histamine- and insulin-activated acid secretion measurements was significant and similar to that after truncal vagotomy and drainage. The measurements were of no practical value for the diagnosis of recurrent duodenal ulcer after vagotomy, but they provided a rationale for selective surgery in patients with duodenal ulcer and patients with recurrent duodenal ulcer after vagotomy.", "PMID": 959759}
{"id": "pubmed23n0032_3595", "title": "The effect of antral distension on acid secretion and plasma gastrin in duodenal ulcer patients.", "content": "The effect of antral balloon distension on acid secretion and the plasma gastrin concentration was studied in 8 duodenal ulcer patients. Antral distension significantly increased the acid secretion to about 30% of the peak acid response to pentagastrin without any change in the plasma gastrin concentration. Antral distension and concomitant intragastric neutralization, with the intention of facilitating release of antral gastrin, produced about the same acid response and did not evoke any plasma gastrin increment. The results suggest that the acid response to antral distension in duodenal ulcer patients is evoked without contribution of the gastrin mechanism, and that the acid response is probably mediated via a pyloro-oxyntic reflex. In this respect the duodenal ulcer patient seems to differ from the healthy subject, in whom antral distension produces no acid response, and from the dog, in which release of gastrin as well as pyloro-oxyntic reflex participate in the acid response to antral distension.", "contents": "The effect of antral distension on acid secretion and plasma gastrin in duodenal ulcer patients. The effect of antral balloon distension on acid secretion and the plasma gastrin concentration was studied in 8 duodenal ulcer patients. Antral distension significantly increased the acid secretion to about 30% of the peak acid response to pentagastrin without any change in the plasma gastrin concentration. Antral distension and concomitant intragastric neutralization, with the intention of facilitating release of antral gastrin, produced about the same acid response and did not evoke any plasma gastrin increment. The results suggest that the acid response to antral distension in duodenal ulcer patients is evoked without contribution of the gastrin mechanism, and that the acid response is probably mediated via a pyloro-oxyntic reflex. In this respect the duodenal ulcer patient seems to differ from the healthy subject, in whom antral distension produces no acid response, and from the dog, in which release of gastrin as well as pyloro-oxyntic reflex participate in the acid response to antral distension.", "PMID": 959760}
{"id": "pubmed23n0032_3596", "title": "Regional ileitis (Crohn's disease). I. Kinetics of bile acid absorption in the perfused ileum.", "content": "Bile acid absorption was studied by steady state perfusion technique in the ileum of 11 patients with regional ileitis (Crohn's disease). By computerizing absorption kinetics the presence of an active transport of glycochenodeoxycholic acid (GCDC) was rendered probable by finding a saturable transport system and a competitive absorption between conjugated bile acids. At the time of investigation 5 patients had no diarrhoea, whereas 6 patients had diarrhoea as defined from the amount of faecal output. In the former group the faecal bile acid excretion was low, the ileal absorption of GCDC high, and judged from the xylose absorption the ileal absorption surface large compared to the latter group, in which the faecal bile acid excretion was high, the ileal absorption of GCDC low, and the ileal absorptive surface small. It is concluded that malabsorption of bile acids in the ileum may be of significant physiological importance in the pathogenesis of diarrhoea in patients with regional ileitis.", "contents": "Regional ileitis (Crohn's disease). I. Kinetics of bile acid absorption in the perfused ileum. Bile acid absorption was studied by steady state perfusion technique in the ileum of 11 patients with regional ileitis (Crohn's disease). By computerizing absorption kinetics the presence of an active transport of glycochenodeoxycholic acid (GCDC) was rendered probable by finding a saturable transport system and a competitive absorption between conjugated bile acids. At the time of investigation 5 patients had no diarrhoea, whereas 6 patients had diarrhoea as defined from the amount of faecal output. In the former group the faecal bile acid excretion was low, the ileal absorption of GCDC high, and judged from the xylose absorption the ileal absorption surface large compared to the latter group, in which the faecal bile acid excretion was high, the ileal absorption of GCDC low, and the ileal absorptive surface small. It is concluded that malabsorption of bile acids in the ileum may be of significant physiological importance in the pathogenesis of diarrhoea in patients with regional ileitis.", "PMID": 959761}
{"id": "pubmed23n0032_3597", "title": "Regional ileitis (Crohn's disease). II. Electrolyte and water movement in the ileum during perfusion with bile acids.", "content": "Electrolyte and water movement was studied by steady state perfusion technique in the ileum of 11 patients with regional ileitis (Crohn's disease). Six patients who at the time of investigation had diarrhoea showed a constant secretion of salts and fluid during perfusion with control perfusate. Per 24 hours, the ileal effluents to colon would exceed the normal amount by 1-2 litres. In the 5 patients without diarrhoea the absorption patterns were normal. Electrolytes and water moved in parallel. Dihydroxy bile acids enhanced secretion or decreased absorption respectively, in the two groups of patients. It is concluded that functional disturbances of salt and water absorption in the inflamed ileum may be of significant physiological importance in the pathogenesis of diarrhoea observed in patients with regional ileitis.", "contents": "Regional ileitis (Crohn's disease). II. Electrolyte and water movement in the ileum during perfusion with bile acids. Electrolyte and water movement was studied by steady state perfusion technique in the ileum of 11 patients with regional ileitis (Crohn's disease). Six patients who at the time of investigation had diarrhoea showed a constant secretion of salts and fluid during perfusion with control perfusate. Per 24 hours, the ileal effluents to colon would exceed the normal amount by 1-2 litres. In the 5 patients without diarrhoea the absorption patterns were normal. Electrolytes and water moved in parallel. Dihydroxy bile acids enhanced secretion or decreased absorption respectively, in the two groups of patients. It is concluded that functional disturbances of salt and water absorption in the inflamed ileum may be of significant physiological importance in the pathogenesis of diarrhoea observed in patients with regional ileitis.", "PMID": 959762}
{"id": "pubmed23n0032_3598", "title": "Fat digestion after jejunoileal bypass operation for obesity.", "content": "Fat digestion in the upper jejunum was studied in 12 obese patients treated by jejunoileal bypass including either 37.5 cm jejunum and 12.5 cm ileum or 12.5 cm jejunum and 37.5 cm ileum. Jejunum was intubated and a test meal ingested before and 1-2 months after operation. Jejunal aspirates were heated immediately to destroy lipase, pooled and assayed for dilution marker, bile acids and total fat. Lipolytic products were determined by thin-layer chromatography and densitometry. The micellar phase was achieved by ultracentrifugation and analysed for total fat. Results. Bile acid concentration and lipolysis decreased after both bypass operations. In accordance with this, micellar solubilization of fat was impaired after operation. Bile acid concentration and micellar solubilization were lower after bypass with a long, than after bypass with a short, ileal loop. Absorption of fat in the duodenum and upper jejunum did not change significantly after any of the operations. Conclusions. Jejunoileal bypass impairs fat digestion, but this does nto contribute significantly to the fat malabsorption after operation.", "contents": "Fat digestion after jejunoileal bypass operation for obesity. Fat digestion in the upper jejunum was studied in 12 obese patients treated by jejunoileal bypass including either 37.5 cm jejunum and 12.5 cm ileum or 12.5 cm jejunum and 37.5 cm ileum. Jejunum was intubated and a test meal ingested before and 1-2 months after operation. Jejunal aspirates were heated immediately to destroy lipase, pooled and assayed for dilution marker, bile acids and total fat. Lipolytic products were determined by thin-layer chromatography and densitometry. The micellar phase was achieved by ultracentrifugation and analysed for total fat. Results. Bile acid concentration and lipolysis decreased after both bypass operations. In accordance with this, micellar solubilization of fat was impaired after operation. Bile acid concentration and micellar solubilization were lower after bypass with a long, than after bypass with a short, ileal loop. Absorption of fat in the duodenum and upper jejunum did not change significantly after any of the operations. Conclusions. Jejunoileal bypass impairs fat digestion, but this does nto contribute significantly to the fat malabsorption after operation.", "PMID": 959763}
{"id": "pubmed23n0032_3599", "title": "Semiquantitative measurement of lithocholic acid compounds in bile from patients with gallstones, before and during treatment with chenodeoxycholic acid.", "content": "A method for two-dimensional thin-layer chromatography of unprocessed bile is described. The method gives distinct spots from lithocholic acid and its compounds with glycine, taurine, and sulfuric acid. The size of the spots is estimated by comparison with standard spots, and the concentrations of individual bile acids are expressed as a fraction of the total content of bile acids in the sample. The concentration of total lithocholic acid was found to be 1-3 (mean 1.5) per cent of the total concentration of bile acids in duodenal bile from 14 healthy persons. It was slightly higher in bile from 6 untreated patients with gallstones, and still higher, 3-7 (mean 4.0) per cent, in bile from the same patients during treatment with chenodeoxycholic acid. The difference between the concentrations of total lithocholic acid in the bile samples from healthy persons and from patients treated with chenodeoxycholic acid was statistically significant. Nearly all the lithocholic acid was conjugated with glycine or taurine, and approximately half of it was sulfated. The increase in total lithocholic acid concentration in the bile from patients consisted of glycine conjugates.", "contents": "Semiquantitative measurement of lithocholic acid compounds in bile from patients with gallstones, before and during treatment with chenodeoxycholic acid. A method for two-dimensional thin-layer chromatography of unprocessed bile is described. The method gives distinct spots from lithocholic acid and its compounds with glycine, taurine, and sulfuric acid. The size of the spots is estimated by comparison with standard spots, and the concentrations of individual bile acids are expressed as a fraction of the total content of bile acids in the sample. The concentration of total lithocholic acid was found to be 1-3 (mean 1.5) per cent of the total concentration of bile acids in duodenal bile from 14 healthy persons. It was slightly higher in bile from 6 untreated patients with gallstones, and still higher, 3-7 (mean 4.0) per cent, in bile from the same patients during treatment with chenodeoxycholic acid. The difference between the concentrations of total lithocholic acid in the bile samples from healthy persons and from patients treated with chenodeoxycholic acid was statistically significant. Nearly all the lithocholic acid was conjugated with glycine or taurine, and approximately half of it was sulfated. The increase in total lithocholic acid concentration in the bile from patients consisted of glycine conjugates.", "PMID": 959764}
{"id": "pubmed23n0032_3600", "title": "Response to jejunal acidification in man. II. Pancreatic, biliary and gastric responses.", "content": "The effect of perfusion of the jejunum with acid on the secretion of bicarbonate, trypsin, and bile into the duodenum has been studied in 10 patients with duodenal ulcer and 8 control subjects and compared with the responses to intravenous infusion of secretin and cholecystokinin-pancreozymin (CCK). Both the secretion of bicarbonate and of trypsin into the duodenum were inversely related to the amount of acid dissipated during jejunal transit, so that if all the acid disappeared during transit of a 30-cm segment of jejunum, the pancreas did not secrete. In both groups of subjects, jejunal acidification elicited a relatively greater secretion of bicarbonate than secretion of trypsin or bile into the duodenum, compared with the response to the exogenous hormones. Jejunal acidification also inhibited the secretion of trypsin in response to concurrently administered exogenous hormones by the control subjects but not in patients with duodenal ulcer. We conclude taht the pancreatic response to acid in the human duodenum depends on exposure of the jejunal mucosa to the amounts of acid which are greater than (quite high, but variable) threshold in all subjects. Under the conditions of this study, patients with duodenal ulcer differed from control in lacking an acid-released jejunal inhibitor of the secretion of trypsin by the pancreas.", "contents": "Response to jejunal acidification in man. II. Pancreatic, biliary and gastric responses. The effect of perfusion of the jejunum with acid on the secretion of bicarbonate, trypsin, and bile into the duodenum has been studied in 10 patients with duodenal ulcer and 8 control subjects and compared with the responses to intravenous infusion of secretin and cholecystokinin-pancreozymin (CCK). Both the secretion of bicarbonate and of trypsin into the duodenum were inversely related to the amount of acid dissipated during jejunal transit, so that if all the acid disappeared during transit of a 30-cm segment of jejunum, the pancreas did not secrete. In both groups of subjects, jejunal acidification elicited a relatively greater secretion of bicarbonate than secretion of trypsin or bile into the duodenum, compared with the response to the exogenous hormones. Jejunal acidification also inhibited the secretion of trypsin in response to concurrently administered exogenous hormones by the control subjects but not in patients with duodenal ulcer. We conclude taht the pancreatic response to acid in the human duodenum depends on exposure of the jejunal mucosa to the amounts of acid which are greater than (quite high, but variable) threshold in all subjects. Under the conditions of this study, patients with duodenal ulcer differed from control in lacking an acid-released jejunal inhibitor of the secretion of trypsin by the pancreas.", "PMID": 959765}
{"id": "pubmed23n0032_3601", "title": "The possible precancerous significance of abnormal cells in gastric cytology specimens: a follow-up study of 545 patients.", "content": "In a series of 545 patients without roentgenological and gastroscopical signs of gastric malignancy, the cytological finding was interpreted as normal (Papanicolaou I) in 351 patients and abnormal (Papanicolaou II-III) in 194 patients. In the abnormal group were included 48 patients with large nuclei and 11 patients with cytologically wrong positive smears (Papanicolaou IV-V), in whom x-ray examination, gastroscopy, and a follow-up study did not reveal any signs of gastric carcinoma. The two groups were collected under uniform principles and studied by the same methods. They were also comparable as to age and sex distribution. In a 15-year follow-up study it was found that 2 patients (0.6%) in the cytologically normal group and 5 (2.6%) in the abnormal group had died of gastric carcinoma. The difference was statistically significant. The 15-year death rate from gastric carcinoma in the cytologically abnormal group was statistically significantly higher than in the expected death rate in the general population of corresponding age and sex distribution.", "contents": "The possible precancerous significance of abnormal cells in gastric cytology specimens: a follow-up study of 545 patients. In a series of 545 patients without roentgenological and gastroscopical signs of gastric malignancy, the cytological finding was interpreted as normal (Papanicolaou I) in 351 patients and abnormal (Papanicolaou II-III) in 194 patients. In the abnormal group were included 48 patients with large nuclei and 11 patients with cytologically wrong positive smears (Papanicolaou IV-V), in whom x-ray examination, gastroscopy, and a follow-up study did not reveal any signs of gastric carcinoma. The two groups were collected under uniform principles and studied by the same methods. They were also comparable as to age and sex distribution. In a 15-year follow-up study it was found that 2 patients (0.6%) in the cytologically normal group and 5 (2.6%) in the abnormal group had died of gastric carcinoma. The difference was statistically significant. The 15-year death rate from gastric carcinoma in the cytologically abnormal group was statistically significantly higher than in the expected death rate in the general population of corresponding age and sex distribution.", "PMID": 959766}
{"id": "pubmed23n0032_3602", "title": "Effect of barium meal on resting gastro-oesophageal sphincter pressure in normal human subjects.", "content": "The effect of ingestion of 250 ml X-ray contrast medium on resting gastrooesophageal sphincter pressure was studied in 10 normal human subjects. Twenty minutes after initiated intake of the contrast medium, a significant fall in sphincter pressure was recorded. In the light of studies on the neurogenic and hormonal regulation of the sphincter pressure it is attempted to account for this effect, and it is concluded that it must be a manifestation of a reflectory relaxation of the sphincter region. From a clinical point of view the consequence must be a demand for standardized conditions with regard to composition and pH of the contrast medium, time schedule and degree of abdominal compression in those cases in which radiological evaluation of the resistance against reflux is called for.", "contents": "Effect of barium meal on resting gastro-oesophageal sphincter pressure in normal human subjects. The effect of ingestion of 250 ml X-ray contrast medium on resting gastrooesophageal sphincter pressure was studied in 10 normal human subjects. Twenty minutes after initiated intake of the contrast medium, a significant fall in sphincter pressure was recorded. In the light of studies on the neurogenic and hormonal regulation of the sphincter pressure it is attempted to account for this effect, and it is concluded that it must be a manifestation of a reflectory relaxation of the sphincter region. From a clinical point of view the consequence must be a demand for standardized conditions with regard to composition and pH of the contrast medium, time schedule and degree of abdominal compression in those cases in which radiological evaluation of the resistance against reflux is called for.", "PMID": 959767}
{"id": "pubmed23n0032_3603", "title": "Serum isoamylases in chronic pancreatitis.", "content": "Serum isoamylases were quantitated separately in 15 patients with chronic pancreatitis. The mean activity of pancreatic isoamylase was 7 U/1 in the patients as compared to 88 U/1 in healtly controls, 8 of the patients completely lacked pancreatic isoamylase and the mean activity in the other was 15 U/1. Salivary isoamylase activities were moderately increased in the patients. Determination of the serum pancreatic isoamylase is a simple screening procedure that should be useful in the diagnosis of exocrine pancreatic insufficiency.", "contents": "Serum isoamylases in chronic pancreatitis. Serum isoamylases were quantitated separately in 15 patients with chronic pancreatitis. The mean activity of pancreatic isoamylase was 7 U/1 in the patients as compared to 88 U/1 in healtly controls, 8 of the patients completely lacked pancreatic isoamylase and the mean activity in the other was 15 U/1. Salivary isoamylase activities were moderately increased in the patients. Determination of the serum pancreatic isoamylase is a simple screening procedure that should be useful in the diagnosis of exocrine pancreatic insufficiency.", "PMID": 959768}
{"id": "pubmed23n0032_3604", "title": "Serum bile acid concentrations in patients with liver disease.", "content": "An enzymatic-fluorimetric method using a highly purified 3alpha-hydroxysteroid dehydrogenase (Sterognost-3alpha, Nyco) was used to determine fasting serum bile acid concentrations on 49 occasions in 43 patients with various liver diseases. A two-hour postprandial bile acid determination was carried out on 29 occasions in 27 of the patients. Fasting bile acid concentration correlated significantly both in cholestatic hepatobiliary and in parenchymatous liver disease to serum bilirubin and aspartate aminotransferase (ASAT) but not to alanine aminotransferase (ALAT), alkaline phosphatase, or albumin. The two-hour postprandial bile acid concentration was above normal in all patients with biochemical and/or histological signs of hepatobiliary disease, also when fasting concentration was within normal limits. In parenchymatous liver disease correlations existed between the two-hour postprandial bile acid concentration and bilirubin, ASAT, and ALAT. The sensitivity of serum bile acid estimation was compared to other liver function tests. Both the fasting and the postprandial serum bile acid concentrations tended to be more sensitive tests of hepatobiliary disease than bilirubin, ASAT and ALAT.", "contents": "Serum bile acid concentrations in patients with liver disease. An enzymatic-fluorimetric method using a highly purified 3alpha-hydroxysteroid dehydrogenase (Sterognost-3alpha, Nyco) was used to determine fasting serum bile acid concentrations on 49 occasions in 43 patients with various liver diseases. A two-hour postprandial bile acid determination was carried out on 29 occasions in 27 of the patients. Fasting bile acid concentration correlated significantly both in cholestatic hepatobiliary and in parenchymatous liver disease to serum bilirubin and aspartate aminotransferase (ASAT) but not to alanine aminotransferase (ALAT), alkaline phosphatase, or albumin. The two-hour postprandial bile acid concentration was above normal in all patients with biochemical and/or histological signs of hepatobiliary disease, also when fasting concentration was within normal limits. In parenchymatous liver disease correlations existed between the two-hour postprandial bile acid concentration and bilirubin, ASAT, and ALAT. The sensitivity of serum bile acid estimation was compared to other liver function tests. Both the fasting and the postprandial serum bile acid concentrations tended to be more sensitive tests of hepatobiliary disease than bilirubin, ASAT and ALAT.", "PMID": 959769}
{"id": "pubmed23n0032_3605", "title": "Familial polycythaemia vera: a study of 3 sisters.", "content": "The familial occurrence of polycythaemia vera (PV) is rare, with only 3 authentic reports in the world literature. This paper describes a family of 8 females, 3 of whom have PV and another has pernicious anaemia. Clinical and haematological data is presented to support the diagnosis of PV and exclude other causes of familial erythrocytosis. Cytogenetic studies were performed in 2 of the patients.", "contents": "Familial polycythaemia vera: a study of 3 sisters. The familial occurrence of polycythaemia vera (PV) is rare, with only 3 authentic reports in the world literature. This paper describes a family of 8 females, 3 of whom have PV and another has pernicious anaemia. Clinical and haematological data is presented to support the diagnosis of PV and exclude other causes of familial erythrocytosis. Cytogenetic studies were performed in 2 of the patients.", "PMID": 959770}
{"id": "pubmed23n0032_3606", "title": "Heparin elimination in uraemic patients on Haemodialysis.", "content": "Heparin (100 U/kg bodyweight) was administered as single i.v. injections, and heparin concentration in plasma determined by polybrene titration. Mean concentration half-life was 74.7 min in the normal group (n = 6), 118.6 min in the nephrectomized patients (n = 5), and 97.8 min in the other uraemic patients (n = 6). The differences between the mean values for the normals and for the 2 patient groups were statistically significant (p is less than 0.001 and p approximately 0.2 respectively). Mean anticoagulant half-life (based on thrombin clotting time) was 64.3, 75.8 and 62.7 min in the 3 groups. The differences between heparin concentration half-life and anticoagulant half-life in the 3 groups were statistically significant. These differences may be partly explained by a significant fall in heparin cofactor activity after injection of heparin. There was a strong positive individual correlation between heparin concentration half-life and anticoagulant half-life in the patients (r = 0.94), but not in the normal group (r = 0.31). There was a strong negative individual correlation between heparin tolerance and heparin concentration half-life in the patients (r = -0.84), but no correlation in the normal group. It is concluded that severely impaired renal function has a significant, but moderate influence on heparin elimination.", "contents": "Heparin elimination in uraemic patients on Haemodialysis. Heparin (100 U/kg bodyweight) was administered as single i.v. injections, and heparin concentration in plasma determined by polybrene titration. Mean concentration half-life was 74.7 min in the normal group (n = 6), 118.6 min in the nephrectomized patients (n = 5), and 97.8 min in the other uraemic patients (n = 6). The differences between the mean values for the normals and for the 2 patient groups were statistically significant (p is less than 0.001 and p approximately 0.2 respectively). Mean anticoagulant half-life (based on thrombin clotting time) was 64.3, 75.8 and 62.7 min in the 3 groups. The differences between heparin concentration half-life and anticoagulant half-life in the 3 groups were statistically significant. These differences may be partly explained by a significant fall in heparin cofactor activity after injection of heparin. There was a strong positive individual correlation between heparin concentration half-life and anticoagulant half-life in the patients (r = 0.94), but not in the normal group (r = 0.31). There was a strong negative individual correlation between heparin tolerance and heparin concentration half-life in the patients (r = -0.84), but no correlation in the normal group. It is concluded that severely impaired renal function has a significant, but moderate influence on heparin elimination.", "PMID": 959771}
{"id": "pubmed23n0032_3607", "title": "Atypical intraerythrocytic pyruvate kinase in some haematological diseases.", "content": "An atypical pyruvate kinase (PK) in erythrocytes was studied in a family with several different haematological diseases: non-spherocytic haemolysis, thrombocytopenia, myelofibrosis and polycythaemic traits. Atypical intraerythrocytic PK was also found in a group of non-related patients with myelofibrosis and polycythaemia vera. The atypical PK was characterised by abnormal urea inhibition and other relevant biochemical parameters.", "contents": "Atypical intraerythrocytic pyruvate kinase in some haematological diseases. An atypical pyruvate kinase (PK) in erythrocytes was studied in a family with several different haematological diseases: non-spherocytic haemolysis, thrombocytopenia, myelofibrosis and polycythaemic traits. Atypical intraerythrocytic PK was also found in a group of non-related patients with myelofibrosis and polycythaemia vera. The atypical PK was characterised by abnormal urea inhibition and other relevant biochemical parameters.", "PMID": 959772}
{"id": "pubmed23n0032_3608", "title": "Platelet consumption and plasma concentration of platelet factor 4 (PF-J).", "content": "In 10 cancer patients platelet concentration, platelet consumption, platelet half-life time and plasms concentrations of platelet factor 4 were determined. A high freguency of thrombocytosis and increased platelet consumption were observed, and the plasms concentrations of platelet factor 4 was increased. However, no significant correlation was found between platelet consumption and the result of a single determination of platelet factor 4 concentrations in plasma.", "contents": "Platelet consumption and plasma concentration of platelet factor 4 (PF-J). In 10 cancer patients platelet concentration, platelet consumption, platelet half-life time and plasms concentrations of platelet factor 4 were determined. A high freguency of thrombocytosis and increased platelet consumption were observed, and the plasms concentrations of platelet factor 4 was increased. However, no significant correlation was found between platelet consumption and the result of a single determination of platelet factor 4 concentrations in plasma.", "PMID": 959773}
{"id": "pubmed23n0032_3609", "title": "Spontaneous and oxalate-induced radial segmentation of the nuclei of lymphocytes from peripheral blood from patients with chronic lymphocytic leukaemia.", "content": "Radial segmentation of nuclei of lymphocytes from peripheral blood from 18 patients with chronic lymphocytic leukaemia was studied in heparinized blood samples incubated with and without oxalate, for 6 h, at 20 degrees C, and 37 degrees C. The yield of radially segmented nuclei was the same after incubation at 20 degrees C as at 37 degrees C. Treatment with sodium oxalate resulted in an increased formation of radial segmentation to the same extent at 20 degrees C as at 37 degrees C. No correlation was found between the size of the lymphocytes and the frequency of radially segmented cells. The number of radially segmented nuclei did not vary with the WBC or Hb value. Cytostatic treatment with chlorambucil and cyclophosphamide did not inhibit the formation of radially segmented nuclei.", "contents": "Spontaneous and oxalate-induced radial segmentation of the nuclei of lymphocytes from peripheral blood from patients with chronic lymphocytic leukaemia. Radial segmentation of nuclei of lymphocytes from peripheral blood from 18 patients with chronic lymphocytic leukaemia was studied in heparinized blood samples incubated with and without oxalate, for 6 h, at 20 degrees C, and 37 degrees C. The yield of radially segmented nuclei was the same after incubation at 20 degrees C as at 37 degrees C. Treatment with sodium oxalate resulted in an increased formation of radial segmentation to the same extent at 20 degrees C as at 37 degrees C. No correlation was found between the size of the lymphocytes and the frequency of radially segmented cells. The number of radially segmented nuclei did not vary with the WBC or Hb value. Cytostatic treatment with chlorambucil and cyclophosphamide did not inhibit the formation of radially segmented nuclei.", "PMID": 959774}
{"id": "pubmed23n0032_3610", "title": "The oxalate-induced radial segmentation of the nuclei in peripheral blood lymphocytes of different size.", "content": "The incidence of oxalate-induced radial-segmented (RS) nuclei in lymphocytes from peripheral blood correlated closely with the diameter of the cell and with the diameter of the nucleus; the majority of RS nuclei were found in large lymphocytes. This observation supports the basic hypotheses, that large lymphocytes from peripheral blood represent a later stage in the cell cycle than small lymphocytes, that the tubulin content of lymphocytes increases during the interphase and that the incidence of RS nuclei reflects the tubulin content of the cells within the various size classes.", "contents": "The oxalate-induced radial segmentation of the nuclei in peripheral blood lymphocytes of different size. The incidence of oxalate-induced radial-segmented (RS) nuclei in lymphocytes from peripheral blood correlated closely with the diameter of the cell and with the diameter of the nucleus; the majority of RS nuclei were found in large lymphocytes. This observation supports the basic hypotheses, that large lymphocytes from peripheral blood represent a later stage in the cell cycle than small lymphocytes, that the tubulin content of lymphocytes increases during the interphase and that the incidence of RS nuclei reflects the tubulin content of the cells within the various size classes.", "PMID": 959775}
{"id": "pubmed23n0032_3611", "title": "Neutrophil defect in multiple myeloma. Studies on intraneutrophilic lysozyme in multiple myeloma and malignant lymphoma.", "content": "Intracellular lysozyme concentration was measured in neutrophilic granulocytes from 25 patients with multiple myeloma. At diagnosis intraneutrophil lysozyme activity was significantly reduced (mean reduction 50%). During clinical remission after 1-4 months of intensive chemotherapy values were normalized. In 18 cases studied at various stages of the disease from 6 to 70 months after diagnosis there was a significant negative correlation between the duration of the disease and neutrophil lysozyme concentration. The decrease in neutrophil lysozyme concentration was significantly correlated to clinical disease activity and the percentage of plasma cells in bone marrow aspirates, whereas there was no correlation between the concentration of M-protein in serum and the neutrophil lysozyme concentration. Plasma lysozyme concentration was normal. In contrast, neutrophil lysozyme concentration was normal in 18 patients with stage III-IV malignant lymphoma. Plasma lysozyme in this group was significantly higher than normal. The difference in neutrophil lysozyme patterns between multiple myeloma and malignant lymphoma supports the hypothesis that the defect in neutrophil maturation seen in malignant blood disorders is directly related to the infiltration of the bone marrow by pathologic cells.", "contents": "Neutrophil defect in multiple myeloma. Studies on intraneutrophilic lysozyme in multiple myeloma and malignant lymphoma. Intracellular lysozyme concentration was measured in neutrophilic granulocytes from 25 patients with multiple myeloma. At diagnosis intraneutrophil lysozyme activity was significantly reduced (mean reduction 50%). During clinical remission after 1-4 months of intensive chemotherapy values were normalized. In 18 cases studied at various stages of the disease from 6 to 70 months after diagnosis there was a significant negative correlation between the duration of the disease and neutrophil lysozyme concentration. The decrease in neutrophil lysozyme concentration was significantly correlated to clinical disease activity and the percentage of plasma cells in bone marrow aspirates, whereas there was no correlation between the concentration of M-protein in serum and the neutrophil lysozyme concentration. Plasma lysozyme concentration was normal. In contrast, neutrophil lysozyme concentration was normal in 18 patients with stage III-IV malignant lymphoma. Plasma lysozyme in this group was significantly higher than normal. The difference in neutrophil lysozyme patterns between multiple myeloma and malignant lymphoma supports the hypothesis that the defect in neutrophil maturation seen in malignant blood disorders is directly related to the infiltration of the bone marrow by pathologic cells.", "PMID": 959776}
{"id": "pubmed23n0032_3612", "title": "Preparation of concentrated platelet suspensions by dehydration against polyethyleneglycol 20,000.", "content": "Gel filtered platelet suspensions were concentrated by repeated dehydration against polyethyleneglycol 20,000 followed by dialysis against slightly hypotonic buffer. This method increased the platelet concentration 2-3 times with a recovery of 80-100%. The final cell suspension closely resembled the original platelet rich plasma as tested by a number of platelet tests.", "contents": "Preparation of concentrated platelet suspensions by dehydration against polyethyleneglycol 20,000. Gel filtered platelet suspensions were concentrated by repeated dehydration against polyethyleneglycol 20,000 followed by dialysis against slightly hypotonic buffer. This method increased the platelet concentration 2-3 times with a recovery of 80-100%. The final cell suspension closely resembled the original platelet rich plasma as tested by a number of platelet tests.", "PMID": 959777}
{"id": "pubmed23n0032_3613", "title": "Cell-mediated cytotoxicity for melanoma tumor cells: detection by a (3H) proline release assay.", "content": "An in vitro lymphocyte-mediated cytotoxicity assay using [3H]proline-labeled target cells is described. The assay, modified from an original procedure of Bean et al., assesses the release of [3-H]proline by filtering the total culture fluid containing both trypsinized tumor cells and effector cells. Filtration is performed with a semiautomatic harvesting device using low suction pressure and large-diameter glass filters. Pretreatment of filters with whole serum diminishes adsorption of cell-free radioactive material considerably and thus increases the sensitivity of the assay. Nearly 100% of the radioactivity could be recovered with this harvesting device. The technique allowed the detection of cytolytic activities of lymphocytes after 6 h of incubation. Lymphocytes from patients with primary malignant melanoma showed a significantly higher cytolytic reactivity (P less than 0.001) than normal donors' lymphocytes against three different melanoma cell lines. In a series of parallel experiments on 36 patients and 18 normal donors, this modification of the [3H]proline test was compared with three different assays: the conventional microcytotoxicity test of Takasugi and Klein, the original [3H]proline microcytotoxicity test of Bean et al., and the viability count of tumor cells.", "contents": "Cell-mediated cytotoxicity for melanoma tumor cells: detection by a (3H) proline release assay. An in vitro lymphocyte-mediated cytotoxicity assay using [3H]proline-labeled target cells is described. The assay, modified from an original procedure of Bean et al., assesses the release of [3-H]proline by filtering the total culture fluid containing both trypsinized tumor cells and effector cells. Filtration is performed with a semiautomatic harvesting device using low suction pressure and large-diameter glass filters. Pretreatment of filters with whole serum diminishes adsorption of cell-free radioactive material considerably and thus increases the sensitivity of the assay. Nearly 100% of the radioactivity could be recovered with this harvesting device. The technique allowed the detection of cytolytic activities of lymphocytes after 6 h of incubation. Lymphocytes from patients with primary malignant melanoma showed a significantly higher cytolytic reactivity (P less than 0.001) than normal donors' lymphocytes against three different melanoma cell lines. In a series of parallel experiments on 36 patients and 18 normal donors, this modification of the [3H]proline test was compared with three different assays: the conventional microcytotoxicity test of Takasugi and Klein, the original [3H]proline microcytotoxicity test of Bean et al., and the viability count of tumor cells.", "PMID": 959779}
{"id": "pubmed23n0032_3614", "title": "Measles virus-induced migration inhibition in vitro of leukocytes from patients with multiple sclerosis.", "content": "The migration inhibition in vitro induced by measles virus material on leukocytes from 17 patients with multiple sclerosis (MS) was compared with that of 8 patients with various other central nervous system diseases and that of 12 healthy blood donors. Significant differences could not be demonstrated. The results do not indicate that MS patients' leukocytes show an altered behaviour to measles virus material in the migration inhibition test.", "contents": "Measles virus-induced migration inhibition in vitro of leukocytes from patients with multiple sclerosis. The migration inhibition in vitro induced by measles virus material on leukocytes from 17 patients with multiple sclerosis (MS) was compared with that of 8 patients with various other central nervous system diseases and that of 12 healthy blood donors. Significant differences could not be demonstrated. The results do not indicate that MS patients' leukocytes show an altered behaviour to measles virus material in the migration inhibition test.", "PMID": 959780}
{"id": "pubmed23n0032_3615", "title": "Sick-leave after road traffic accidents.", "content": "425 road traffic casualties, all covered by the National Insurance Scheme, were followed up regarding duration of inability to work. The average for the whole group was 35 days. There was a close relation between seriousness of injury and time off work. Motorcyclists had the longest time off work and cyclists injured in bicycle accidents the shortest. Casualties with orthopaedic injuries were off work for 120 days, on average, and accounted for 67% of the total paid sick-leave, although they constituted only 20% of those injured. The responsibility for the treatment of trauma is discussed.", "contents": "Sick-leave after road traffic accidents. 425 road traffic casualties, all covered by the National Insurance Scheme, were followed up regarding duration of inability to work. The average for the whole group was 35 days. There was a close relation between seriousness of injury and time off work. Motorcyclists had the longest time off work and cyclists injured in bicycle accidents the shortest. Casualties with orthopaedic injuries were off work for 120 days, on average, and accounted for 67% of the total paid sick-leave, although they constituted only 20% of those injured. The responsibility for the treatment of trauma is discussed.", "PMID": 959781}
{"id": "pubmed23n0032_3616", "title": "The North Karelia Project: a programme for community control of cardiovascular diseases.", "content": "In this article the background and principles of the intervention and evaluation of the North Karelia Project are presented. The project is a comprehensive community control programme concerning cardiovascular diseases in the province of North Karelia, Eastern Finland. In the baseline survey of the project (1972), 25-59-year-old males had a particularly high of CVD level risk factors; there were 54% current smokers, the mean cholesterol was 269 mg% and the mean casual blood pressure was 147/90 mmHg. According to the myocardial infarction register the incidence rate of acute myocardial infraction was, during the first year of the project (1972), 13.8 per thousand among 30-64-yearold males. During the first 2 1/2 of the intervention the percentage of current smokers decreased among males from 54 to 42. The percentage of males using low-fat milk increased from 17 to 48. The percentage of males who had had their blood pressure measured during the previous half year increased from 28 to 56 and those under antihypertensive drug treatment from 3.1 to 9.1. Local groups for secondary prevention of myocardial infarction were established all over the province. On the basis of the accumulated experience and theoretical considerations, the project plan has futher been elaborated.", "contents": "The North Karelia Project: a programme for community control of cardiovascular diseases. In this article the background and principles of the intervention and evaluation of the North Karelia Project are presented. The project is a comprehensive community control programme concerning cardiovascular diseases in the province of North Karelia, Eastern Finland. In the baseline survey of the project (1972), 25-59-year-old males had a particularly high of CVD level risk factors; there were 54% current smokers, the mean cholesterol was 269 mg% and the mean casual blood pressure was 147/90 mmHg. According to the myocardial infarction register the incidence rate of acute myocardial infraction was, during the first year of the project (1972), 13.8 per thousand among 30-64-yearold males. During the first 2 1/2 of the intervention the percentage of current smokers decreased among males from 54 to 42. The percentage of males using low-fat milk increased from 17 to 48. The percentage of males who had had their blood pressure measured during the previous half year increased from 28 to 56 and those under antihypertensive drug treatment from 3.1 to 9.1. Local groups for secondary prevention of myocardial infarction were established all over the province. On the basis of the accumulated experience and theoretical considerations, the project plan has futher been elaborated.", "PMID": 959783}
{"id": "pubmed23n0032_3617", "title": "Hypertension programme of the North Karelia Project.", "content": "The present article describes the background and principles of a programme for community control of hypertension, which is part of the North Karelia project--a comprehensive community programme for the control of cardiovascular diseases. The hypertension programme also forms part of an international co-operative study by WHO. The programme consists of a community intervention integrated with the service structure. The methods used include health education of the public, education of health service personnel, organization of a service for the spreading of information and also for screening, treatment and follow-up. Every hypertensive person in the community is registered in the hypertension register and checked on annually. The evaluation is made mainly on baseline and terminal survey data from the intervention area and a reference area, the hypertension register, a myocardial infarction register and a stroke register in the intervention area.", "contents": "Hypertension programme of the North Karelia Project. The present article describes the background and principles of a programme for community control of hypertension, which is part of the North Karelia project--a comprehensive community programme for the control of cardiovascular diseases. The hypertension programme also forms part of an international co-operative study by WHO. The programme consists of a community intervention integrated with the service structure. The methods used include health education of the public, education of health service personnel, organization of a service for the spreading of information and also for screening, treatment and follow-up. Every hypertensive person in the community is registered in the hypertension register and checked on annually. The evaluation is made mainly on baseline and terminal survey data from the intervention area and a reference area, the hypertension register, a myocardial infarction register and a stroke register in the intervention area.", "PMID": 959785}
{"id": "pubmed23n0032_3618", "title": "Health situation and related social conditions among 66-year-old Finnish men.", "content": "The aims of the study were to reveal the extent of physical activity, nutritional habits, smoking and alcohol consumption and the relation of these to previous occupation among recently retired men. The number of men belonging to various health risk groups was estimated on the basis of the results. About 10% had inadequate nutritional habits as judged from their food expenses and number of daily hot meals and about 20% practised physical exercise in the way which is considered necessary for the improvement of cardiovascular performance and maximal oxygen uptake. Only a small minority had been requested to undertake physical exercise. About 18% lived alone. The problems were more pronounced among the men retired from manual occupations compared with men whose main occupation had been of a sedentary nature. The results indicate that a considerable proportion of elderly men would need more advice and more adequate services in order to improve their daily living habits. Any such measures should preferably be started before retirement as the living habits adopted during the earlier periods of life seem to remain unchanged in old age. The results are based on an interview study carried out among 137 66-year-old men representing about 90% of the men of that age group living in Jyv\u00e4skyl\u00e4, a town of 60000 inhabitants in Central Finland.", "contents": "Health situation and related social conditions among 66-year-old Finnish men. The aims of the study were to reveal the extent of physical activity, nutritional habits, smoking and alcohol consumption and the relation of these to previous occupation among recently retired men. The number of men belonging to various health risk groups was estimated on the basis of the results. About 10% had inadequate nutritional habits as judged from their food expenses and number of daily hot meals and about 20% practised physical exercise in the way which is considered necessary for the improvement of cardiovascular performance and maximal oxygen uptake. Only a small minority had been requested to undertake physical exercise. About 18% lived alone. The problems were more pronounced among the men retired from manual occupations compared with men whose main occupation had been of a sedentary nature. The results indicate that a considerable proportion of elderly men would need more advice and more adequate services in order to improve their daily living habits. Any such measures should preferably be started before retirement as the living habits adopted during the earlier periods of life seem to remain unchanged in old age. The results are based on an interview study carried out among 137 66-year-old men representing about 90% of the men of that age group living in Jyv\u00e4skyl\u00e4, a town of 60000 inhabitants in Central Finland.", "PMID": 959786}
{"id": "pubmed23n0032_3619", "title": "On pensioners' circumstances in a large Swedish city. A report from Malm\u00f6.", "content": "In 1972-1973 the authors carried out a field-work programme in a surburban area covered by the 4th Welfare District of Malm\u00f6 with the objective of charting the general circumstances among the pensioners living there. Such surveys should be pursued in such a way that immediate needs would be disclosed and met. The findings were intended to form a basis for future improvement projects. As expected, the results show the necessity of tracking down hidden needs, often underestimated by the pensioners themselves, especially those in poor health and of limited means. Within the framework of a permanent field-work programme, those people must be made more aware of their own interests and rights.", "contents": "On pensioners' circumstances in a large Swedish city. A report from Malm\u00f6. In 1972-1973 the authors carried out a field-work programme in a surburban area covered by the 4th Welfare District of Malm\u00f6 with the objective of charting the general circumstances among the pensioners living there. Such surveys should be pursued in such a way that immediate needs would be disclosed and met. The findings were intended to form a basis for future improvement projects. As expected, the results show the necessity of tracking down hidden needs, often underestimated by the pensioners themselves, especially those in poor health and of limited means. Within the framework of a permanent field-work programme, those people must be made more aware of their own interests and rights.", "PMID": 959787}
{"id": "pubmed23n0032_3620", "title": "Retirement and psychic investments.", "content": "An attempt has been made to investigate the effect of retirement on the amount of psychic investment in the different sections of the social field. A further investigation was made on the application of the disengagement theory and the theory of role flexibility to the present material. The results do not support either of the theories. The material consisted of 28 pensioners, who were sent 3 questionnaires: on retirement, and 3 months and 1 year later. In processing the material, factor analysis and group analysis were primarily employed. In the group analysis, the observation units (84) (each answer from each subject) were divided into three groups. The present results showed a considerable degree of stability in the subjects, irrespective of their retirement; 82% had remained in the same group for all three questionnaires.", "contents": "Retirement and psychic investments. An attempt has been made to investigate the effect of retirement on the amount of psychic investment in the different sections of the social field. A further investigation was made on the application of the disengagement theory and the theory of role flexibility to the present material. The results do not support either of the theories. The material consisted of 28 pensioners, who were sent 3 questionnaires: on retirement, and 3 months and 1 year later. In processing the material, factor analysis and group analysis were primarily employed. In the group analysis, the observation units (84) (each answer from each subject) were divided into three groups. The present results showed a considerable degree of stability in the subjects, irrespective of their retirement; 82% had remained in the same group for all three questionnaires.", "PMID": 959788}
{"id": "pubmed23n0032_3621", "title": "Transmission of vibration in the hand-arm system with special reference to changes in compression force and acceleration.", "content": "The transmission of longitudinal vibration in the hand-arm system of five subjects was investigated. Altogether 405 individual tests were made. Vibration was measured with an accelerometer (weight 0.4 g) fixed in turn to the wrist, the elbow, and the upper arm by means of a supporting device (weight 34 g). A handle with strain gauges attached was used to study the effect of compression force (10, 20 and 40 N) and constant acceleration (1, 3 and 10 g) on the transmission of vibration at frequencies from 20 to 630 Hz. In the curves recorded, sharp dips appeared which were evidently caused by resonances from the soft tissues of the hand. However in the hand-arm system no common resonance frequency was observed that would harmfully affect the health of workers. Vibration in the hand-arm system was attenuated at an average of 3 dB per octave at the frequencies between 20 and 100 Hz. Between 100 and 630 Hz the attenuation was about 6 dB per octave in the wrist and 10 dB per octave in the elbow and upper arm. At the frequency of 630 Hz the attenuation was hence about 35 dB in the wrist and about 45 DB in the elbow. The attenuation of vibration in the elbow joint was 2 to 4 dB at all frequencies. The hand-arm system appears to be linear at the acceleration range considered; the increase in handle vibration by, e.g., 10 dB also increased vibration in the hand by 10 dB. When the grip strength was increased fourfold, i.e., 12 dB, vibration increased only 3 to 5 dB in the hand-arm system. Thus changing the weight of a vibrating tool does not reduce vibration enough. Therefore attempts to reduce vibration should concentrate on the mechanical parts of the engines.", "contents": "Transmission of vibration in the hand-arm system with special reference to changes in compression force and acceleration. The transmission of longitudinal vibration in the hand-arm system of five subjects was investigated. Altogether 405 individual tests were made. Vibration was measured with an accelerometer (weight 0.4 g) fixed in turn to the wrist, the elbow, and the upper arm by means of a supporting device (weight 34 g). A handle with strain gauges attached was used to study the effect of compression force (10, 20 and 40 N) and constant acceleration (1, 3 and 10 g) on the transmission of vibration at frequencies from 20 to 630 Hz. In the curves recorded, sharp dips appeared which were evidently caused by resonances from the soft tissues of the hand. However in the hand-arm system no common resonance frequency was observed that would harmfully affect the health of workers. Vibration in the hand-arm system was attenuated at an average of 3 dB per octave at the frequencies between 20 and 100 Hz. Between 100 and 630 Hz the attenuation was about 6 dB per octave in the wrist and 10 dB per octave in the elbow and upper arm. At the frequency of 630 Hz the attenuation was hence about 35 dB in the wrist and about 45 DB in the elbow. The attenuation of vibration in the elbow joint was 2 to 4 dB at all frequencies. The hand-arm system appears to be linear at the acceleration range considered; the increase in handle vibration by, e.g., 10 dB also increased vibration in the hand by 10 dB. When the grip strength was increased fourfold, i.e., 12 dB, vibration increased only 3 to 5 dB in the hand-arm system. Thus changing the weight of a vibrating tool does not reduce vibration enough. Therefore attempts to reduce vibration should concentrate on the mechanical parts of the engines.", "PMID": 959789}
{"id": "pubmed23n0032_3622", "title": "Systolic time intervals as a measure of left ventricular function in viscose rayon workers exposed to carbon disulfide.", "content": "In a group of viscose rayon workers exposed to carbon disulfide the systolic time intervals were calculated. They were determined from the simultaneous recording of an ECG, a phonocardiogram, and arterial pulse tracings. Results indicate a shortened left ventricular ejection time (lvet) and a prolonged isovolumetric contraction time (ICT), together with an increase in the ratio of ICT to LVET. These alterations, which occurred in the absence of any clinical signs or symptoms of heart disease, are interpreted as a consequence of an impaired left ventricular contractility, comparable to mild coronary dysfunction. Literature data support the hypothesis that the biochemical mechanisms involved in the effect of carbon disulfide on myocardial cells are (a) an interference with energy metabolism from the inadequate availability of thiamine and nicotinamide or a direct inhibition of cytochrome or (b) an interference with catecholamine metabolism and defective hormonal control of the energy utilization process. Impaired left ventricular function in rayon viscose workers exposed to carbon disulfide represents an early and highly sensitive sign of carbon disulfide intoxication, and the use of systolic time intervals as a noninvasive measure of myocardial performance may be a useful method for monitoring carbon disulfide exposed people.", "contents": "Systolic time intervals as a measure of left ventricular function in viscose rayon workers exposed to carbon disulfide. In a group of viscose rayon workers exposed to carbon disulfide the systolic time intervals were calculated. They were determined from the simultaneous recording of an ECG, a phonocardiogram, and arterial pulse tracings. Results indicate a shortened left ventricular ejection time (lvet) and a prolonged isovolumetric contraction time (ICT), together with an increase in the ratio of ICT to LVET. These alterations, which occurred in the absence of any clinical signs or symptoms of heart disease, are interpreted as a consequence of an impaired left ventricular contractility, comparable to mild coronary dysfunction. Literature data support the hypothesis that the biochemical mechanisms involved in the effect of carbon disulfide on myocardial cells are (a) an interference with energy metabolism from the inadequate availability of thiamine and nicotinamide or a direct inhibition of cytochrome or (b) an interference with catecholamine metabolism and defective hormonal control of the energy utilization process. Impaired left ventricular function in rayon viscose workers exposed to carbon disulfide represents an early and highly sensitive sign of carbon disulfide intoxication, and the use of systolic time intervals as a noninvasive measure of myocardial performance may be a useful method for monitoring carbon disulfide exposed people.", "PMID": 959790}
{"id": "pubmed23n0032_3623", "title": "Occupational lead exposure in Finland. VI. Final report.", "content": "Between 1970 and 1973, 2,209 workers representing 30 different types of work were studied in Finland for lead exposure. The indicator of lead exposure was the blood lead concentrations (Pb-B) of the workers. The highest recommended value for Pb-B in Finland, 70 mug/100 ml of blood, was exceeded in the following types of work: PVC plastic manufacturing, storage tank manufacturing and repairing, machine shop work, treating metal surfaces, paint manufacturing, car radiator repairing, spray painting, machine shop work (railway), and storage battery repair. The types of work with the highest average exposure were lead scrap smelting (Pb-B median 79 mug/100 ml, range 35--118), storage battery manufacturing (Pb-B median 66 mug/100 ml, range 19--101), metal founding, (Pb-B median 53 mug/100 ml, range 6--108) shipbreaking (Pb-B median 49 mug/100 ml, range 26--106) crystal glass manufacturing (Pb-B median 41 mug/100 ml, range 12--82), car radiator repairing (Pb-B median 38 mug/100 ml, range 17--83), and PVC plastic manufacturing (Pb-B median 37 mug/100 ml, range 10--126). During the past 5 years cases of clincial lead poisoning have occurred in all of these types of work, and the patients received workmen's compensation. The usefulness of the national poisoning register in predicting the relative hazard of lead exposure in the types of work studied was evaluated with the aid of rank order correlation statistics. The analysis showed that the poisoning register is a useful indicator of lead exposure in the most exposed types of work. However, the present survey also revealed work in which the hazard had escaped recognition in this country: metal founding, car repairing, and car radiator repairing, for example. Although it seems possible to predict the most heavily exposed work types from national poisoning registers only, the detection of workers with less severe manifestations of toxic effects, or somtimes even poisoning, in other types of work first requires a systematic survey of all types of work with possible lead exposure, and then regular monitoring of all exposed workers.", "contents": "Occupational lead exposure in Finland. VI. Final report. Between 1970 and 1973, 2,209 workers representing 30 different types of work were studied in Finland for lead exposure. The indicator of lead exposure was the blood lead concentrations (Pb-B) of the workers. The highest recommended value for Pb-B in Finland, 70 mug/100 ml of blood, was exceeded in the following types of work: PVC plastic manufacturing, storage tank manufacturing and repairing, machine shop work, treating metal surfaces, paint manufacturing, car radiator repairing, spray painting, machine shop work (railway), and storage battery repair. The types of work with the highest average exposure were lead scrap smelting (Pb-B median 79 mug/100 ml, range 35--118), storage battery manufacturing (Pb-B median 66 mug/100 ml, range 19--101), metal founding, (Pb-B median 53 mug/100 ml, range 6--108) shipbreaking (Pb-B median 49 mug/100 ml, range 26--106) crystal glass manufacturing (Pb-B median 41 mug/100 ml, range 12--82), car radiator repairing (Pb-B median 38 mug/100 ml, range 17--83), and PVC plastic manufacturing (Pb-B median 37 mug/100 ml, range 10--126). During the past 5 years cases of clincial lead poisoning have occurred in all of these types of work, and the patients received workmen's compensation. The usefulness of the national poisoning register in predicting the relative hazard of lead exposure in the types of work studied was evaluated with the aid of rank order correlation statistics. The analysis showed that the poisoning register is a useful indicator of lead exposure in the most exposed types of work. However, the present survey also revealed work in which the hazard had escaped recognition in this country: metal founding, car repairing, and car radiator repairing, for example. Although it seems possible to predict the most heavily exposed work types from national poisoning registers only, the detection of workers with less severe manifestations of toxic effects, or somtimes even poisoning, in other types of work first requires a systematic survey of all types of work with possible lead exposure, and then regular monitoring of all exposed workers.", "PMID": 959791}
{"id": "pubmed23n0032_3624", "title": "Use of breath analysis to monitor methylene chloride exposure.", "content": "Twenty male and female subjects were exposed repetitively to methylene chloride (CH2Cl2) vapor, 50, 100, 250, and 500 ppm, for 1,3, or 7.5 h in a controlled-environment chamber. Postexposure alveolar breath samples were collected in small glass breath tubes and analyzed for CH2Cl2 by gas chromatography. From these data is a series of breath CH2Cl2 excretion curves were constructed that can be used to estimate the magnitude of a recent exposure. The CH2CL2 breath concentration in the immediate postexposure period accurately reflected the vapor concentration to which the subjects had been exposed most recently. Breath samples collected 1--2 h following exposure were accurate indicators of the time-weighted average vapor exposure experienced by the subjects during the previous 8 h of occupational exposure. Breath analysis offers a practical, noninvasive method for monitoring occupational exposure to CH2Cl2.", "contents": "Use of breath analysis to monitor methylene chloride exposure. Twenty male and female subjects were exposed repetitively to methylene chloride (CH2Cl2) vapor, 50, 100, 250, and 500 ppm, for 1,3, or 7.5 h in a controlled-environment chamber. Postexposure alveolar breath samples were collected in small glass breath tubes and analyzed for CH2Cl2 by gas chromatography. From these data is a series of breath CH2Cl2 excretion curves were constructed that can be used to estimate the magnitude of a recent exposure. The CH2CL2 breath concentration in the immediate postexposure period accurately reflected the vapor concentration to which the subjects had been exposed most recently. Breath samples collected 1--2 h following exposure were accurate indicators of the time-weighted average vapor exposure experienced by the subjects during the previous 8 h of occupational exposure. Breath analysis offers a practical, noninvasive method for monitoring occupational exposure to CH2Cl2.", "PMID": 959792}
{"id": "pubmed23n0032_3625", "title": "Use of chlorophenols as fungicides in sawmills.", "content": "Several commercial formulations of chlorophenols used as fungicides in sawmills were found to contain impurities, some of which are known to be much more toxic than the main products. The most frequent impurities were chlorinated phenoxyphenols, chlorinated diphenyl ethers, chlorinated dibenzofurans, and chlorinated dibenzodioxins. Samples of wood dust from several positions in two Swedish sawmills were analyzed. The results suggest that the impurities in the chlorophenol formulations are enriched in the work environment in relation to the chlorophenols themselves, probably because of the low volatility and high chemical stability of the impurities. The effect of various methods of fungicide application on the work environment is discussed.", "contents": "Use of chlorophenols as fungicides in sawmills. Several commercial formulations of chlorophenols used as fungicides in sawmills were found to contain impurities, some of which are known to be much more toxic than the main products. The most frequent impurities were chlorinated phenoxyphenols, chlorinated diphenyl ethers, chlorinated dibenzofurans, and chlorinated dibenzodioxins. Samples of wood dust from several positions in two Swedish sawmills were analyzed. The results suggest that the impurities in the chlorophenol formulations are enriched in the work environment in relation to the chlorophenols themselves, probably because of the low volatility and high chemical stability of the impurities. The effect of various methods of fungicide application on the work environment is discussed.", "PMID": 959793}
{"id": "pubmed23n0032_3626", "title": "X-ray fluorescence analysis of lead in human skeleton in vivo.", "content": "The lead concentration in the skeleton of living man was measured by X-ray fluorescence analysis. Five former workers from a metal industry were studied. The mean lead concentration in their skeletons was estimated to be 62 mug/g with a standard error of +/- 5 mug/g. A comparison with the \"normal\" skeletal concentrations of lead in people from southern Sweden showed the skeletal concentrations of the men studied to be about three to nine times higher.", "contents": "X-ray fluorescence analysis of lead in human skeleton in vivo. The lead concentration in the skeleton of living man was measured by X-ray fluorescence analysis. Five former workers from a metal industry were studied. The mean lead concentration in their skeletons was estimated to be 62 mug/g with a standard error of +/- 5 mug/g. A comparison with the \"normal\" skeletal concentrations of lead in people from southern Sweden showed the skeletal concentrations of the men studied to be about three to nine times higher.", "PMID": 959794}
{"id": "pubmed23n0032_3627", "title": "Exposure to anesthetic gases and ethanol during work in operating rooms.", "content": "The concentration of halothane and ethanol in operating rooms was measured during 37 routine operations performed in nine different departments of surgery at six different hospitals. The time-weighted halothane concentrations in the respiratory zones of anesthetic and surgical nurses were 0.3--34.0 ppm (time-weighted average 7.2 ppm) and 0.1--9.2 ppm (time-weighted average 2.5 ppm), respectively, in the different operating departments. The corresponding ethanol concentrations were 0.3--36.5 ppm (time-weighted average 12.5 ppm) for anesthetic nurses and 1.5--46.6 ppm (time-weighted average 15.3 ppm) for surgical nurses. The anesthetic technique influences the exposure of the operating staff to anesthetic gases, but it does not affect exposure to ethanol. In controlled experiments volunteers were exposed to low concentrations of halothane or ethanol. About 60% of both substances was retained. The content of ethanol in the end-expired air approached zero within a few minutes after the end of exposure, while low residual concentrations of halothane were demonstrable for more than 1 h. Although exposure to ethanol is insignificant in relation to the metabolic capacity of the body, ethanol indicates the presence of volatile disinfectant components, and its spread through the room atmosphere should be kept in mind when the ventilation of operating rooms is designed. The effective elimination of airborne pollutants in operating rooms calls for good general ventilation in conjunction with local exhaust close to the sources of anesthetic gas leakage. General ventilation mainly affects the concentration of substances well-mixed with the room atmosphere, such as volatile disinfectant components and anesthetic vapor that has spread beyond the actual work zones of the medical staff. For a significant reduction in the concentration of anesthetic gases in the respiratory zones of the medical staff, the gases must be vented at the source of leakage. Since airborne anesthetics occur not only in operating rooms, general ventilation has to meet certain minimum requirements also in anesthetic induction rooms and recovery rooms. Operating rooms and anesthetic induction rooms must also be supplied with local exhaust systems.", "contents": "Exposure to anesthetic gases and ethanol during work in operating rooms. The concentration of halothane and ethanol in operating rooms was measured during 37 routine operations performed in nine different departments of surgery at six different hospitals. The time-weighted halothane concentrations in the respiratory zones of anesthetic and surgical nurses were 0.3--34.0 ppm (time-weighted average 7.2 ppm) and 0.1--9.2 ppm (time-weighted average 2.5 ppm), respectively, in the different operating departments. The corresponding ethanol concentrations were 0.3--36.5 ppm (time-weighted average 12.5 ppm) for anesthetic nurses and 1.5--46.6 ppm (time-weighted average 15.3 ppm) for surgical nurses. The anesthetic technique influences the exposure of the operating staff to anesthetic gases, but it does not affect exposure to ethanol. In controlled experiments volunteers were exposed to low concentrations of halothane or ethanol. About 60% of both substances was retained. The content of ethanol in the end-expired air approached zero within a few minutes after the end of exposure, while low residual concentrations of halothane were demonstrable for more than 1 h. Although exposure to ethanol is insignificant in relation to the metabolic capacity of the body, ethanol indicates the presence of volatile disinfectant components, and its spread through the room atmosphere should be kept in mind when the ventilation of operating rooms is designed. The effective elimination of airborne pollutants in operating rooms calls for good general ventilation in conjunction with local exhaust close to the sources of anesthetic gas leakage. General ventilation mainly affects the concentration of substances well-mixed with the room atmosphere, such as volatile disinfectant components and anesthetic vapor that has spread beyond the actual work zones of the medical staff. For a significant reduction in the concentration of anesthetic gases in the respiratory zones of the medical staff, the gases must be vented at the source of leakage. Since airborne anesthetics occur not only in operating rooms, general ventilation has to meet certain minimum requirements also in anesthetic induction rooms and recovery rooms. Operating rooms and anesthetic induction rooms must also be supplied with local exhaust systems.", "PMID": 959795}
{"id": "pubmed23n0032_3628", "title": "Depressed cholinesterase activities among farm workers in New Jersey.", "content": "The widespread use of organophosphate pesticides creates the possibility of excessive exposure of migrant farm workers to these compounds. Blood cholinesterase determinations were used to compare the organophosphate pesticide exposure of 57 migrant farm workers with that of 35 controls. Frequently reported symptoms of the farm workers which might be related to pesticide exposure were also studied, including headaches, dizziness, loss of weight, nausea, and a general feeling of weakness or loss of energy. Significantly depressed cholinesterase activities were found in the farm workers, with 10.5% of the farm workers having values below the lower limit of normal. There was no significant relationship between frequently reported symptoms of the farm workers and depressed cholinesterase levels.", "contents": "Depressed cholinesterase activities among farm workers in New Jersey. The widespread use of organophosphate pesticides creates the possibility of excessive exposure of migrant farm workers to these compounds. Blood cholinesterase determinations were used to compare the organophosphate pesticide exposure of 57 migrant farm workers with that of 35 controls. Frequently reported symptoms of the farm workers which might be related to pesticide exposure were also studied, including headaches, dizziness, loss of weight, nausea, and a general feeling of weakness or loss of energy. Significantly depressed cholinesterase activities were found in the farm workers, with 10.5% of the farm workers having values below the lower limit of normal. There was no significant relationship between frequently reported symptoms of the farm workers and depressed cholinesterase levels.", "PMID": 959812}
{"id": "pubmed23n0032_3629", "title": "Studies of Fructus schizandrae. IV. Isolation and determination of the active compounds (in lowering high SGPT levels) of Schizandra chinensis Baill.", "content": "The alcoholic extracts of the kernels of Schizandra chinensis Baill have been reported to be capable of lowering elevated SGPT levels in mice induced by CCl4 and inhibiting the central nervous system. Seven pharmacological active compounds have been isolated from the alcoholic extracts by column chromatography. Five of them are identified as wuweizisu C (I), wuweizisu B (II), wuweizisu A (III), wuweizichun B (IV) and wuweizichun A (V). They are all derivatives of the dibenzo (a, c) cyclooctene system, among which compounds I and IV have hitherto not been reported in literature. Ozonolysis of III and subsequent oxidation with H2O2 yields meso-3,4-dimethyladipic acid. Pyrolytic syn-elimination of V acetate and oxidation of the resultant olefin (V-4) affords a diketone (V-5) with molecular weight of 446. According to these results and based on the NMR spectra, the two methyl groups at C-6 and C-7 of compound I--V must be cis-oriented.", "contents": "Studies of Fructus schizandrae. IV. Isolation and determination of the active compounds (in lowering high SGPT levels) of Schizandra chinensis Baill. The alcoholic extracts of the kernels of Schizandra chinensis Baill have been reported to be capable of lowering elevated SGPT levels in mice induced by CCl4 and inhibiting the central nervous system. Seven pharmacological active compounds have been isolated from the alcoholic extracts by column chromatography. Five of them are identified as wuweizisu C (I), wuweizisu B (II), wuweizisu A (III), wuweizichun B (IV) and wuweizichun A (V). They are all derivatives of the dibenzo (a, c) cyclooctene system, among which compounds I and IV have hitherto not been reported in literature. Ozonolysis of III and subsequent oxidation with H2O2 yields meso-3,4-dimethyladipic acid. Pyrolytic syn-elimination of V acetate and oxidation of the resultant olefin (V-4) affords a diketone (V-5) with molecular weight of 446. According to these results and based on the NMR spectra, the two methyl groups at C-6 and C-7 of compound I--V must be cis-oriented.", "PMID": 959810}
{"id": "pubmed23n0032_3630", "title": "Subjective measurement of high-order aberrations of the eye.", "content": "We used an apparatus similar to Tscherning's aberroscope, and analyzed subjects' drawings to obtain the wave aberration surfaces of 55 eyes. This analysis permitted a Taylor series representation of the wave aberrations to terms of the fourth order. The results revealed a wide variety in type and severity of high-order aberrations in which \"cylindrical\" aberrations were prominent and cases of classical spherical aberrations were rare. We computed the monochromatic modulation transfer function curves for the range of observations. The overall findings suggest a more prominent role for monochromatic high-order aberrations in degrading the visual image than has hitherto been assumed.", "contents": "Subjective measurement of high-order aberrations of the eye. We used an apparatus similar to Tscherning's aberroscope, and analyzed subjects' drawings to obtain the wave aberration surfaces of 55 eyes. This analysis permitted a Taylor series representation of the wave aberrations to terms of the fourth order. The results revealed a wide variety in type and severity of high-order aberrations in which \"cylindrical\" aberrations were prominent and cases of classical spherical aberrations were rare. We computed the monochromatic modulation transfer function curves for the range of observations. The overall findings suggest a more prominent role for monochromatic high-order aberrations in degrading the visual image than has hitherto been assumed.", "PMID": 959814}
{"id": "pubmed23n0032_3631", "title": "Transplanted neural tissue develops connections with host rat brain.", "content": "Superior collicular fragments transplanted from fetal to newborn rat brains develop complex internal organization and receive visual afferents from the host providing they lie sufficiently close to the host visual pathways. This system allows investigation in vivo of special affinities between cells of the mammalian central nervous system.", "contents": "Transplanted neural tissue develops connections with host rat brain. Superior collicular fragments transplanted from fetal to newborn rat brains develop complex internal organization and receive visual afferents from the host providing they lie sufficiently close to the host visual pathways. This system allows investigation in vivo of special affinities between cells of the mammalian central nervous system.", "PMID": 959815}
{"id": "pubmed23n0032_3632", "title": "Potential new artificial sweetener from study of structure-taste relationships.", "content": "4-(Methoxymethyl)-1,4-cyclohexadiene-1-carboxaldehyde syn-oxime is a new sweetening agent developed by systematic synthesis and taste evaluation of 80 new oximes analogous to the little-used osime sweetener, perillartine.", "contents": "Potential new artificial sweetener from study of structure-taste relationships. 4-(Methoxymethyl)-1,4-cyclohexadiene-1-carboxaldehyde syn-oxime is a new sweetening agent developed by systematic synthesis and taste evaluation of 80 new oximes analogous to the little-used osime sweetener, perillartine.", "PMID": 959816}
{"id": "pubmed23n0032_3633", "title": "Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.", "content": "Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.", "contents": "Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture. Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.", "PMID": 959817}
{"id": "pubmed23n0032_3634", "title": "Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.", "content": "Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.", "contents": "Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture. Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.", "PMID": 959818}
{"id": "pubmed23n0032_3635", "title": "The insertion of DNA into vaccinia virus.", "content": "Cells infected with vaccinia virus in the presence of hydroxyurea (HU), which blocks DNA replication, were examined in thin sections by electron microscopy at intervals after removal of HU. Dense, fibrillar material was observed at the orifice formed just before closure of the membrane constituting the envelope of the immature form of the virus. It is concluded that synchrony of assembly enabled stages in the condensation and insertion of viral deoxyribonucleoprotein to be observed. The mechanism appears to be similar to that encountered in morphologic studies of herpes simplex virus and in biochemical studies of poliovirus, adenovirus, and several bacteriophages.", "contents": "The insertion of DNA into vaccinia virus. Cells infected with vaccinia virus in the presence of hydroxyurea (HU), which blocks DNA replication, were examined in thin sections by electron microscopy at intervals after removal of HU. Dense, fibrillar material was observed at the orifice formed just before closure of the membrane constituting the envelope of the immature form of the virus. It is concluded that synchrony of assembly enabled stages in the condensation and insertion of viral deoxyribonucleoprotein to be observed. The mechanism appears to be similar to that encountered in morphologic studies of herpes simplex virus and in biochemical studies of poliovirus, adenovirus, and several bacteriophages.", "PMID": 959819}
{"id": "pubmed23n0032_3636", "title": "Benzo(a)pyrene diol epoxides as intermediates in nucleic acid binding in vitro and in vivo.", "content": "Evidence has been obtained that a specific isomer of a diol epoxide derivative of benzo(a)pyrene, (+/-)-7 beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, is an intermediate in the binding of benzo(a)pyrene to RNA in cultured bovine bronchial mucosa. An adduct is formed between position 10 of this derivative and the 2-amino group of guanine.", "contents": "Benzo(a)pyrene diol epoxides as intermediates in nucleic acid binding in vitro and in vivo. Evidence has been obtained that a specific isomer of a diol epoxide derivative of benzo(a)pyrene, (+/-)-7 beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, is an intermediate in the binding of benzo(a)pyrene to RNA in cultured bovine bronchial mucosa. An adduct is formed between position 10 of this derivative and the 2-amino group of guanine.", "PMID": 959820}
{"id": "pubmed23n0032_3637", "title": "Cytoplasmic aldo-keto reductases: a class of drug metabolizing enzymes.", "content": "Aldehyde and ketone xenobiotic substances are preferentially reduced to alcohols by cytoplasmic enzymes in mammals. These enzymes are widely distributed in the tissues, have broad substrate specificities, have similar physical-chemical characteristics, and require reduced nicotinamide adenine dinucleotide as cofactor for the reductions. These reductases define a system of detoxification for aldehyde and ketone groups.", "contents": "Cytoplasmic aldo-keto reductases: a class of drug metabolizing enzymes. Aldehyde and ketone xenobiotic substances are preferentially reduced to alcohols by cytoplasmic enzymes in mammals. These enzymes are widely distributed in the tissues, have broad substrate specificities, have similar physical-chemical characteristics, and require reduced nicotinamide adenine dinucleotide as cofactor for the reductions. These reductases define a system of detoxification for aldehyde and ketone groups.", "PMID": 959821}
{"id": "pubmed23n0032_3638", "title": "Histones and G banding of chromosomes.", "content": "Polylysine, polyarginine, and histones H1, H2A, H2B, and H3 inhibit Giemsa staining and chromosome banding by binding to DNA and preventing side stacking of the positively charged thiazine dyes to the negatively charged phosphate groups on DNA. This is a nonspecific effect and does not of itself provide evidence for a role of histones in G banding. The question of whether histones are involved in chromosome banding is reviewed.", "contents": "Histones and G banding of chromosomes. Polylysine, polyarginine, and histones H1, H2A, H2B, and H3 inhibit Giemsa staining and chromosome banding by binding to DNA and preventing side stacking of the positively charged thiazine dyes to the negatively charged phosphate groups on DNA. This is a nonspecific effect and does not of itself provide evidence for a role of histones in G banding. The question of whether histones are involved in chromosome banding is reviewed.", "PMID": 959822}
{"id": "pubmed23n0032_3639", "title": "Calcium release from skeletal muscle sarcoplasmic reticulum: site of action of dantrolene sodium.", "content": "The muscle relaxant dantrolene sodium acts directly and specifically on skeletal muscle, unlike other pharmacological agents which affect the central nervous system or act at the nueromuscular junction. Dantrolene sodium markedly suppresses the release of calcium previously sequestered by skeletal, but not cardiac, muscle sarcoplasmic reticulum. No effect in the total amount of calcium accumulated was found. In situ, the drug may reduce the amount of calcium necessary for muscle contraction.", "contents": "Calcium release from skeletal muscle sarcoplasmic reticulum: site of action of dantrolene sodium. The muscle relaxant dantrolene sodium acts directly and specifically on skeletal muscle, unlike other pharmacological agents which affect the central nervous system or act at the nueromuscular junction. Dantrolene sodium markedly suppresses the release of calcium previously sequestered by skeletal, but not cardiac, muscle sarcoplasmic reticulum. No effect in the total amount of calcium accumulated was found. In situ, the drug may reduce the amount of calcium necessary for muscle contraction.", "PMID": 959824}
{"id": "pubmed23n0032_3640", "title": "Photochemotherapy: identification of a metabolite of 4, 5', 8-trimethylpsoralen.", "content": "A compound, 4, 8-dimethyl, 5'-carboxypsoralen (DMeCP), has been identified in mouse urine as a major metabolite of the photoactive drug, 4, 5', 8-trimethylpsoralen (TMeP). This drug is widely used in the treatment of vitiligo and psoriasis. DMeCP is fluorescent, and nonphotosensitizing when tested on guinea pig skin. DMeCP also occurs in the urine of human patients receiving TMeP orally.", "contents": "Photochemotherapy: identification of a metabolite of 4, 5', 8-trimethylpsoralen. A compound, 4, 8-dimethyl, 5'-carboxypsoralen (DMeCP), has been identified in mouse urine as a major metabolite of the photoactive drug, 4, 5', 8-trimethylpsoralen (TMeP). This drug is widely used in the treatment of vitiligo and psoriasis. DMeCP is fluorescent, and nonphotosensitizing when tested on guinea pig skin. DMeCP also occurs in the urine of human patients receiving TMeP orally.", "PMID": 959825}
{"id": "pubmed23n0032_3641", "title": "H-Y male antigen: detection on eight-cell mouse embryos.", "content": "The H-Y (male) antigen is expressed on 50 percent of eight-cell embryos (2 days old) of the mouse, strain C57BL/6. This work provides additional evidence that H-Y expression is not dependent on male differentiation.", "contents": "H-Y male antigen: detection on eight-cell mouse embryos. The H-Y (male) antigen is expressed on 50 percent of eight-cell embryos (2 days old) of the mouse, strain C57BL/6. This work provides additional evidence that H-Y expression is not dependent on male differentiation.", "PMID": 959826}
{"id": "pubmed23n0032_3642", "title": "Coronary arterial smooth muscle contraction by a substance released from platelets: evidence that it is thromboxane A2.", "content": "When human platelets are aggregated by thrombin, material is released that rapidly contracts strips of spirally cut porcine coronary artery. Prevention of the contraction by indomethacin suggested mediation by a prostaglandin. The contraction produced by aggregating platelets was unlike those produced by prostaglandins E2, F2alpha, G2, or H2, but resembled that evoked by thromboxane A2, which is formed by platelets during aggregation.", "contents": "Coronary arterial smooth muscle contraction by a substance released from platelets: evidence that it is thromboxane A2. When human platelets are aggregated by thrombin, material is released that rapidly contracts strips of spirally cut porcine coronary artery. Prevention of the contraction by indomethacin suggested mediation by a prostaglandin. The contraction produced by aggregating platelets was unlike those produced by prostaglandins E2, F2alpha, G2, or H2, but resembled that evoked by thromboxane A2, which is formed by platelets during aggregation.", "PMID": 959827}
{"id": "pubmed23n0032_3643", "title": "Microbial degradation of condensed tannins.", "content": "A strain of Penicillium adametzi Zaleski was isolated from enrichment cultures with condensed tannins as the carbon source. Low-molecular-weight condensed tannins, extracted and purified from Pinus radiata bark, were used as substrates for quantitative growth measurements on this fungus in defined culture conditions.", "contents": "Microbial degradation of condensed tannins. A strain of Penicillium adametzi Zaleski was isolated from enrichment cultures with condensed tannins as the carbon source. Low-molecular-weight condensed tannins, extracted and purified from Pinus radiata bark, were used as substrates for quantitative growth measurements on this fungus in defined culture conditions.", "PMID": 959828}
{"id": "pubmed23n0032_3644", "title": "Behavioral fever in newborn rabbits.", "content": "Rabbit pups, 12 to 72 hours old, did not develop a fever when injected intraperitoneally with a pyrogen and maintained at an ambient temperature of 32 degrees C for 2 hours. When placed in a thermally graded alleyway, animals injected with pyrogen selected gradient positions that represented significantly higher temperatures than controls injected with saline (40.4 degrees in contrast to 36.4 degrees C). Allowing the pups to remain at their selected positions for 5 minutes caused a significant increase in the rectal temperatures of the pyrogen-injected pups but not that of the controls. Thus, newborn rabbits will develop a fever by behavioral means after a single injection of an exogenous pyrogen.", "contents": "Behavioral fever in newborn rabbits. Rabbit pups, 12 to 72 hours old, did not develop a fever when injected intraperitoneally with a pyrogen and maintained at an ambient temperature of 32 degrees C for 2 hours. When placed in a thermally graded alleyway, animals injected with pyrogen selected gradient positions that represented significantly higher temperatures than controls injected with saline (40.4 degrees in contrast to 36.4 degrees C). Allowing the pups to remain at their selected positions for 5 minutes caused a significant increase in the rectal temperatures of the pyrogen-injected pups but not that of the controls. Thus, newborn rabbits will develop a fever by behavioral means after a single injection of an exogenous pyrogen.", "PMID": 959829}
{"id": "pubmed23n0032_3645", "title": "Hardy-Weinberg law: asymptotic approach to a generalized form.", "content": "The equilibrium frequencies of a generalized Hardy-Weinberg law are approached at a geometric rate under assortative mating, irrespective of the initial genotypic frequencies. The asymptotic form is similar to that of Wright, and the pattern of assortative mating is based on deviations from the mean genotypic value.", "contents": "Hardy-Weinberg law: asymptotic approach to a generalized form. The equilibrium frequencies of a generalized Hardy-Weinberg law are approached at a geometric rate under assortative mating, irrespective of the initial genotypic frequencies. The asymptotic form is similar to that of Wright, and the pattern of assortative mating is based on deviations from the mean genotypic value.", "PMID": 959830}
{"id": "pubmed23n0032_3646", "title": "The effect of stimulus sequence on the waveform of the cortical event-related potential.", "content": "The waveform of the cortical event-related potential is extremely sensitive to variations in the sequence of stimuli preceding the eliciting event. The waveform changes were manifested primarily in the amplitudes of the negative component of the potential that peaked at 200 milliseconds, the positive component that peaked at 300 milliseconds, and the slow-wave components. A quantitative model was developed relating the waveform changes to changes in event expectancy. Expectancy is assumed to depend on a decaying memory for events within the prior sequence, the specific structure of the sequence, and the global probability of event occurrence. For stimuli relevant to the task, the less expected the stimulus the larger the amplitudes of late components of the event-related potentials.", "contents": "The effect of stimulus sequence on the waveform of the cortical event-related potential. The waveform of the cortical event-related potential is extremely sensitive to variations in the sequence of stimuli preceding the eliciting event. The waveform changes were manifested primarily in the amplitudes of the negative component of the potential that peaked at 200 milliseconds, the positive component that peaked at 300 milliseconds, and the slow-wave components. A quantitative model was developed relating the waveform changes to changes in event expectancy. Expectancy is assumed to depend on a decaying memory for events within the prior sequence, the specific structure of the sequence, and the global probability of event occurrence. For stimuli relevant to the task, the less expected the stimulus the larger the amplitudes of late components of the event-related potentials.", "PMID": 959831}
{"id": "pubmed23n0032_3647", "title": "Successful immunization against the sexual stages of Plasmodium gallinaceum.", "content": "Gametocyte infectivity and oocyst development of the avian malaria parasite, Plasmodium gallinaceum, can be reduced or eliminated in mosquitoes by immunizing the chickens on which the mosquitoes feed with infected red blood cells that have been treated with formalin or x-rays. Protection of the mosquito appears to be related to the immobilization of the microgametes in its gut and is associated with the immunoglobulin G fraction of serum.", "contents": "Successful immunization against the sexual stages of Plasmodium gallinaceum. Gametocyte infectivity and oocyst development of the avian malaria parasite, Plasmodium gallinaceum, can be reduced or eliminated in mosquitoes by immunizing the chickens on which the mosquitoes feed with infected red blood cells that have been treated with formalin or x-rays. Protection of the mosquito appears to be related to the immobilization of the microgametes in its gut and is associated with the immunoglobulin G fraction of serum.", "PMID": 959832}
{"id": "pubmed23n0032_3648", "title": "Myosin synthesis increased by electrical stimulation of skeletal muscle cell cultures.", "content": "When cultures of skeletal muscle cells of the chick embryo are subjected to repetitive, electrical stimulation, the contractions increase the amount of protein produced by these cells. The increase is greater for contractile proteins such as myosin heavy chain than for total cellular protein. This demonstrates that in a culture system of skeletal muscle cells that have differentiated in the absence of innervation, one can elicit the protein synthetic response associated with skeletal muscle hypertrophy in vivo.", "contents": "Myosin synthesis increased by electrical stimulation of skeletal muscle cell cultures. When cultures of skeletal muscle cells of the chick embryo are subjected to repetitive, electrical stimulation, the contractions increase the amount of protein produced by these cells. The increase is greater for contractile proteins such as myosin heavy chain than for total cellular protein. This demonstrates that in a culture system of skeletal muscle cells that have differentiated in the absence of innervation, one can elicit the protein synthetic response associated with skeletal muscle hypertrophy in vivo.", "PMID": 959833}
{"id": "pubmed23n0032_3649", "title": "Biological impact of small air ions.", "content": "The thrust of the experimental data presented here is that small air ions are biologically active. There is convincing evidence that both negative and positive ions (i) inhibit growth of bacteria and fungi on solid media; (ii) exert a lethal effect on vegetative forms of bacteria suspended in water when opportunity is provided for contact of cells and ions; and (iii) reduce the viable count of bacterial aerosols. Through physical action, ions of either charge upset the stability of aerolosized bacterial suspensions and, in addition, have a direct lethal effect which is more prominent with negative ions than with positive ions. With regard to the serotonin hypothesis of air ions action, the situation is more complex. The essential fact is that mice and rats display a charge-related metabolic response to air ions and this phenomenon also occurs in humans. Because serotonin is such a potent hormone, the ultimate functional changes incident to air ion action are impressive and account for the signs of symptoms of the sharav syndrome. Alterations in the cumulative mortality rate with three experimental respiratory disease in the mouse also are charge-dependent, positive ions routinely exercising a detrimental effect. Further, in the case of mice infected with influenza virus, ion-deprivation increases the cumulative mortality rate. Since ion depletion is a constant concomitant of modern urban life, one reasonably may speculate about comparable inimical effects on humans.", "contents": "Biological impact of small air ions. The thrust of the experimental data presented here is that small air ions are biologically active. There is convincing evidence that both negative and positive ions (i) inhibit growth of bacteria and fungi on solid media; (ii) exert a lethal effect on vegetative forms of bacteria suspended in water when opportunity is provided for contact of cells and ions; and (iii) reduce the viable count of bacterial aerosols. Through physical action, ions of either charge upset the stability of aerolosized bacterial suspensions and, in addition, have a direct lethal effect which is more prominent with negative ions than with positive ions. With regard to the serotonin hypothesis of air ions action, the situation is more complex. The essential fact is that mice and rats display a charge-related metabolic response to air ions and this phenomenon also occurs in humans. Because serotonin is such a potent hormone, the ultimate functional changes incident to air ion action are impressive and account for the signs of symptoms of the sharav syndrome. Alterations in the cumulative mortality rate with three experimental respiratory disease in the mouse also are charge-dependent, positive ions routinely exercising a detrimental effect. Further, in the case of mice infected with influenza virus, ion-deprivation increases the cumulative mortality rate. Since ion depletion is a constant concomitant of modern urban life, one reasonably may speculate about comparable inimical effects on humans.", "PMID": 959834}
{"id": "pubmed23n0032_3650", "title": "Flow-field-flow fractionation: a versatile new separation method.", "content": "Flow-field-fractionation is a new separation technique that seems likely to have broad applicability. Its theoretical scope includes any solute for which one can find a solvent and a semipermeable membrane. The principles on which the technique is based are presented. Some experiments with polystyrene beads, viruses, and proteins verify that retention depends solely on diffusion coefficients.", "contents": "Flow-field-flow fractionation: a versatile new separation method. Flow-field-fractionation is a new separation technique that seems likely to have broad applicability. Its theoretical scope includes any solute for which one can find a solvent and a semipermeable membrane. The principles on which the technique is based are presented. Some experiments with polystyrene beads, viruses, and proteins verify that retention depends solely on diffusion coefficients.", "PMID": 959835}
{"id": "pubmed23n0032_3651", "title": "Identification of specific changes in the pattern of brain protein synthesis after training.", "content": "Double labeling studies with [3H]valine and [14C]valine were used to investigate the pattern of protein synthesis in the brains of goldfish. The protein fractions in three bands (alpha, beta, and gamma) on sodium dodecyl sulfate-polyacrylamide gels indicate that more valine was incorporated in the brains of goldfish that had been trained in a vestibular conditioning task than in the brains of untrained fish or fish trained in a variety of control behavioral situation. Changes in the pattern of labeling were localized in the cytoplasmic fraction of the brain; no increases in labeling occurred in either the nuclear or synaptosomal components. The results suggest that a specific change occurs in the pattern of protein synthesis in the brain after the acquistion of a new behavior.", "contents": "Identification of specific changes in the pattern of brain protein synthesis after training. Double labeling studies with [3H]valine and [14C]valine were used to investigate the pattern of protein synthesis in the brains of goldfish. The protein fractions in three bands (alpha, beta, and gamma) on sodium dodecyl sulfate-polyacrylamide gels indicate that more valine was incorporated in the brains of goldfish that had been trained in a vestibular conditioning task than in the brains of untrained fish or fish trained in a variety of control behavioral situation. Changes in the pattern of labeling were localized in the cytoplasmic fraction of the brain; no increases in labeling occurred in either the nuclear or synaptosomal components. The results suggest that a specific change occurs in the pattern of protein synthesis in the brain after the acquistion of a new behavior.", "PMID": 959837}
{"id": "pubmed23n0032_3652", "title": "Abnormal visual resolution in the Siamese cat.", "content": "When tested behaviorally, Siamese cats display marked differences in contrast sensitivity compared to ordinary cats. Overall sensitivity is depressed, the high-frequency cutoff point is lower, and there is less falloff in sensitivity at low spatial frequencies. Optical factors may contribute to these differences, or they may be attributable to the well-established anatomical abnormalities within the visual system of the Siamese cat.", "contents": "Abnormal visual resolution in the Siamese cat. When tested behaviorally, Siamese cats display marked differences in contrast sensitivity compared to ordinary cats. Overall sensitivity is depressed, the high-frequency cutoff point is lower, and there is less falloff in sensitivity at low spatial frequencies. Optical factors may contribute to these differences, or they may be attributable to the well-established anatomical abnormalities within the visual system of the Siamese cat.", "PMID": 959839}
{"id": "pubmed23n0032_3653", "title": "The clonal evolution of tumor cell populations.", "content": "It is proposed that most neoplasms arise from a single cell of origin, and tumor progression results from acquired genetic variability within the original clone allowing sequential selection of more aggressive sublines. Tumor cell populations are apparently more genetically unstable than normal cells, perhaps from activation of specific gene loci in the neoplasm, continued presence of carcinogen, or even nutritional deficiencies within the tumor. The acquired genetic insta0ility and associated selection process, most readily recognized cytogenetically, results in advanced human malignancies being highly individual karyotypically and biologically. Hence, each patient's cancer may require individual specific therapy, and even this may be thwarted by emergence of a genetically variant subline resistant to the treatment. More research should be directed toward understanding and controlling the evolutionary process in tumors before it reaches the late stage usually seen in clinical cancer.", "contents": "The clonal evolution of tumor cell populations. It is proposed that most neoplasms arise from a single cell of origin, and tumor progression results from acquired genetic variability within the original clone allowing sequential selection of more aggressive sublines. Tumor cell populations are apparently more genetically unstable than normal cells, perhaps from activation of specific gene loci in the neoplasm, continued presence of carcinogen, or even nutritional deficiencies within the tumor. The acquired genetic insta0ility and associated selection process, most readily recognized cytogenetically, results in advanced human malignancies being highly individual karyotypically and biologically. Hence, each patient's cancer may require individual specific therapy, and even this may be thwarted by emergence of a genetically variant subline resistant to the treatment. More research should be directed toward understanding and controlling the evolutionary process in tumors before it reaches the late stage usually seen in clinical cancer.", "PMID": 959840}
{"id": "pubmed23n0032_3654", "title": "Man-made radionuclides and sedimentation in the Hudson River estuary.", "content": "Recently deposited fine-grained sediments in the Hudson River estuary contain radionuclides from global fallout produced by atmospheric bomb tests as well as from low-level releases of a local nuclear reactor. Accumulation rates of these nuclides are dependent on rates of sediment deposition and vary with location in the estuary by more than two orders of magnitude. Within the Hudson estuary, New York harbor is currently the zone of most rapid deposition of sediments containing radionuclides, some of which were released from a nuclear reactor about 60 kilometers upstream of the harbor.", "contents": "Man-made radionuclides and sedimentation in the Hudson River estuary. Recently deposited fine-grained sediments in the Hudson River estuary contain radionuclides from global fallout produced by atmospheric bomb tests as well as from low-level releases of a local nuclear reactor. Accumulation rates of these nuclides are dependent on rates of sediment deposition and vary with location in the estuary by more than two orders of magnitude. Within the Hudson estuary, New York harbor is currently the zone of most rapid deposition of sediments containing radionuclides, some of which were released from a nuclear reactor about 60 kilometers upstream of the harbor.", "PMID": 959844}
{"id": "pubmed23n0032_3655", "title": "Bat mortality: pesticide poisoning and migratory stress.", "content": "Organochlorine residues in the fat of young Mexican free-tailed bats, Tadarida brasiliensis, reached the brain and caused symptoms of poisoning after the fat mobilization that takes place during migratory flight was simulated. These chemical body burdens were obtained naturally under free-living conditions at the maternity roost. The data obtained support the hypothesis that pesticides have contributed to recent declines in populations of this bat.", "contents": "Bat mortality: pesticide poisoning and migratory stress. Organochlorine residues in the fat of young Mexican free-tailed bats, Tadarida brasiliensis, reached the brain and caused symptoms of poisoning after the fat mobilization that takes place during migratory flight was simulated. These chemical body burdens were obtained naturally under free-living conditions at the maternity roost. The data obtained support the hypothesis that pesticides have contributed to recent declines in populations of this bat.", "PMID": 959845}
{"id": "pubmed23n0032_3656", "title": "Formation and transport of secondary air pollutants: ozone and aerosols in the St. Louis urban plume.", "content": "Emissions from metropolitan St. Louis caused reduced visibilities and concentrations of ozone in excess of the federal ambient standard (0.08 part per million) 160 kilometers or more downwind of the city on 18 July 1975. Atmospheric production of ozone and visibility-reducing aerosols continues long after their primary precursors have been diluted to low concentrations.", "contents": "Formation and transport of secondary air pollutants: ozone and aerosols in the St. Louis urban plume. Emissions from metropolitan St. Louis caused reduced visibilities and concentrations of ozone in excess of the federal ambient standard (0.08 part per million) 160 kilometers or more downwind of the city on 18 July 1975. Atmospheric production of ozone and visibility-reducing aerosols continues long after their primary precursors have been diluted to low concentrations.", "PMID": 959846}
{"id": "pubmed23n0032_3657", "title": "Structural basis for ON-and OFF-center responses in retinal ganglion cells.", "content": "The inner plexiform layer of the mammalian retina has a bisublaminar organization determined by restricted branching of the terminals of cone bipolar cells and dendrites of class I (large) and class II (small) ganglion cells. Comparison of dendritic field diameters and receptive fiedl center sizes of large ganglion cells suggests that neural circuitry in sublamina a conveys \"OFF\"-center properties and connections in sublamina b \"ON\"-center properties to retinal ganglion cells.", "contents": "Structural basis for ON-and OFF-center responses in retinal ganglion cells. The inner plexiform layer of the mammalian retina has a bisublaminar organization determined by restricted branching of the terminals of cone bipolar cells and dendrites of class I (large) and class II (small) ganglion cells. Comparison of dendritic field diameters and receptive fiedl center sizes of large ganglion cells suggests that neural circuitry in sublamina a conveys \"OFF\"-center properties and connections in sublamina b \"ON\"-center properties to retinal ganglion cells.", "PMID": 959847}
{"id": "pubmed23n0032_3658", "title": "Hyaluronidase-induced reductions in myocardial infarct size.", "content": "The size of myocardial infarctions following coronary artery occlusion in the rat was determined directly by measurement of creatine phosphokinase activity in homogenized whole left ventricles and by planimetric measurement of the area of the infarctions in histologic sections of serial slices of the left ventricles. Hyaluronidase was shown to produce significant reductions in expected infarct size both 48 hours and 3 weeks after occlusion without impairing fibrosis during the healing phase. Thus, the amount of myocardial necrosis that follows a coronary artery occlusion has been shown directly to be amenable to reduction with a pharmacological intervention.", "contents": "Hyaluronidase-induced reductions in myocardial infarct size. The size of myocardial infarctions following coronary artery occlusion in the rat was determined directly by measurement of creatine phosphokinase activity in homogenized whole left ventricles and by planimetric measurement of the area of the infarctions in histologic sections of serial slices of the left ventricles. Hyaluronidase was shown to produce significant reductions in expected infarct size both 48 hours and 3 weeks after occlusion without impairing fibrosis during the healing phase. Thus, the amount of myocardial necrosis that follows a coronary artery occlusion has been shown directly to be amenable to reduction with a pharmacological intervention.", "PMID": 959848}
{"id": "pubmed23n0032_3659", "title": "Nuclear inclusions in Paget's disease of bone.", "content": "The ultrastructure of bone cells was examined in biopsies from 18 patients with Paget's disease of bone and from 60 patients with a variety of other bone diseases. A characteristic nuclear inclusion was found in the osteoclasts of each patient with Paget's disease. The nuclear inclusion most closely resembles viral nucleocapsids of the measles type.", "contents": "Nuclear inclusions in Paget's disease of bone. The ultrastructure of bone cells was examined in biopsies from 18 patients with Paget's disease of bone and from 60 patients with a variety of other bone diseases. A characteristic nuclear inclusion was found in the osteoclasts of each patient with Paget's disease. The nuclear inclusion most closely resembles viral nucleocapsids of the measles type.", "PMID": 959849}
{"id": "pubmed23n0032_3660", "title": "Depletion of brain catecholamines: failure of ocular dominance shift after monocular occlusion in kittens.", "content": "Monocularly deprived kittens were compared with littermates that had had their eyelids sutured for the same time but that had, in addition, been treated with 6-hydroxydopamine to deplete their forebrains of catecholamines. The visual cortices of all the catecholamine-depleted kittens showed high proportions of binocular neurons, in contrast to the control group, most of whose visual cortical neurons were driven exclusively by the nondeprived eye. Catecholamines may play an important role in the maintenance of cortical plasticity during the critical period.", "contents": "Depletion of brain catecholamines: failure of ocular dominance shift after monocular occlusion in kittens. Monocularly deprived kittens were compared with littermates that had had their eyelids sutured for the same time but that had, in addition, been treated with 6-hydroxydopamine to deplete their forebrains of catecholamines. The visual cortices of all the catecholamine-depleted kittens showed high proportions of binocular neurons, in contrast to the control group, most of whose visual cortical neurons were driven exclusively by the nondeprived eye. Catecholamines may play an important role in the maintenance of cortical plasticity during the critical period.", "PMID": 959850}
{"id": "pubmed23n0032_3661", "title": "Responses of infants to visually presented objects.", "content": "The reaching behavior of some 60 infants between 7 and 23 days of age was studied. Contrary to some other reports, the infants did not respond differently to a visually presented, graspable, solid object than to its two-dimensional representation.", "contents": "Responses of infants to visually presented objects. The reaching behavior of some 60 infants between 7 and 23 days of age was studied. Contrary to some other reports, the infants did not respond differently to a visually presented, graspable, solid object than to its two-dimensional representation.", "PMID": 959851}
{"id": "pubmed23n0032_3662", "title": "Sexual dimorphism in vocal control areas of the songbird brain.", "content": "In canaries and zebra finches, three vocal control areas in the brain are strikingly larger in males than in females. A fourth, area X of the lobus parolfactorius, is well developed in males of both species, less well developed in femal canaries, and absent or not recognizable in femal zebra finches. These size differences correlate well with differences in singing behavior. Males of both species learn song by reference to auditory information, and females do not normally sing. Exogenous testosterone induces singing in female canaries but not in female zebra finches. This is believed to be the first report of such gross sexual dimorphism in a vertebrate brain.", "contents": "Sexual dimorphism in vocal control areas of the songbird brain. In canaries and zebra finches, three vocal control areas in the brain are strikingly larger in males than in females. A fourth, area X of the lobus parolfactorius, is well developed in males of both species, less well developed in femal canaries, and absent or not recognizable in femal zebra finches. These size differences correlate well with differences in singing behavior. Males of both species learn song by reference to auditory information, and females do not normally sing. Exogenous testosterone induces singing in female canaries but not in female zebra finches. This is believed to be the first report of such gross sexual dimorphism in a vertebrate brain.", "PMID": 959852}
{"id": "pubmed23n0032_3663", "title": "[Potassium and sodium electrolyte ratio in the myocardium following death by hanging and electric current].", "content": "The amount of the electrolytes of potassium and sodium contained in different parts of both the chambers and the septa between them was investigated flame photometrically in practically sound persons who died on account of a mechanical asphyxiation by hanging--25 cases--and of an electric accident--12 cases. Statistically significant changes in the eletrolytes of potassium and sodium were detected in cardiac muscle in cases of an electrotrauma as against hanging. In connexion with the changes in the above electrolytes the fragmentation of the muscle fibers of the heart was enquired into. No strict correlational dependency between the changes of electrolyte balance and the degree of fragmentation was found.", "contents": "[Potassium and sodium electrolyte ratio in the myocardium following death by hanging and electric current]. The amount of the electrolytes of potassium and sodium contained in different parts of both the chambers and the septa between them was investigated flame photometrically in practically sound persons who died on account of a mechanical asphyxiation by hanging--25 cases--and of an electric accident--12 cases. Statistically significant changes in the eletrolytes of potassium and sodium were detected in cardiac muscle in cases of an electrotrauma as against hanging. In connexion with the changes in the above electrolytes the fragmentation of the muscle fibers of the heart was enquired into. No strict correlational dependency between the changes of electrolyte balance and the degree of fragmentation was found.", "PMID": 959863}
{"id": "pubmed23n0032_3664", "title": "Overcoming inertia in the planning of a burn care facility.", "content": "Various problems, involving money, space, personnel, and their relationship to the hospital administration, can be associated with the establishment of a Burn Unit. However, these problems can be overcome. During the creation of a Burn Unit, consultation regarding the design features of the unit is particularly important.", "contents": "Overcoming inertia in the planning of a burn care facility. Various problems, involving money, space, personnel, and their relationship to the hospital administration, can be associated with the establishment of a Burn Unit. However, these problems can be overcome. During the creation of a Burn Unit, consultation regarding the design features of the unit is particularly important.", "PMID": 959864}
{"id": "pubmed23n0032_3665", "title": "Problems in management of the pregnant patient with rheumatic heart disease and valve prosthesis.", "content": "The clinical course, through pregnancy and delivery, of a 30-year-old woman with rheumatic heart disease and a prosthetic mitral valve is presented. Despite maternal development of congestive cardiac failure and atrial fibrillation, the delivery of a healthy infant was achieved. The problems encountered during pregnancy and delivery in patients with rheumatic heart disease and prosthetic valves are discussed. These include the management of long-term anticoagulant therapy, prophylaxis against rheumatic fever and subacute bacterial endocarditis, impaired cardiac function, atrial fibrillation, breast feeding, and contraception as they relate both to the mother and the fetus and infant.", "contents": "Problems in management of the pregnant patient with rheumatic heart disease and valve prosthesis. The clinical course, through pregnancy and delivery, of a 30-year-old woman with rheumatic heart disease and a prosthetic mitral valve is presented. Despite maternal development of congestive cardiac failure and atrial fibrillation, the delivery of a healthy infant was achieved. The problems encountered during pregnancy and delivery in patients with rheumatic heart disease and prosthetic valves are discussed. These include the management of long-term anticoagulant therapy, prophylaxis against rheumatic fever and subacute bacterial endocarditis, impaired cardiac function, atrial fibrillation, breast feeding, and contraception as they relate both to the mother and the fetus and infant.", "PMID": 959866}
{"id": "pubmed23n0032_3666", "title": "Case finding of unrelated disease in the orthopedic clinic.", "content": "Protocols for detecting hypertension and pelvic disease were established in the orthopedic outpatient department. A significant percentage (hypertension, 20%; pelvic disease, 10%) of the patients were found to have undiagnosed pathologic conditions. The cost to the orthopedist of such case finding was minimal in terms of time and money. Methods of securing adequate follow-up are discussed.", "contents": "Case finding of unrelated disease in the orthopedic clinic. Protocols for detecting hypertension and pelvic disease were established in the orthopedic outpatient department. A significant percentage (hypertension, 20%; pelvic disease, 10%) of the patients were found to have undiagnosed pathologic conditions. The cost to the orthopedist of such case finding was minimal in terms of time and money. Methods of securing adequate follow-up are discussed.", "PMID": 959867}
{"id": "pubmed23n0032_3667", "title": "Avascular necrosis of the body of the talus after combined medial and lateral release of congenital clubfoot.", "content": "A retrospective study was made of 203 patients with 321 congenital clubfoot deformities who had at least one operative procedure at the Shriner's Hospital in Greenville, South Carolina, in a period from 1944 to 1973. The five cases of avascular necrosis of the talus occurred among the 35 patients having combined medial and lateral releases. These five clubfeet were recurrent and recalcitrant and required extensive soft tissue release to achieve sustained correction. It is recommended that dissection of the subtalar joint, except at the talonavicular joint, be avoided and that extensive lateral ankle dissection, especially when the sinus tarsi is included, should not be performed simultaneously with the medial release unless absolutely necessary to achieve the desired anatomic relationship.", "contents": "Avascular necrosis of the body of the talus after combined medial and lateral release of congenital clubfoot. A retrospective study was made of 203 patients with 321 congenital clubfoot deformities who had at least one operative procedure at the Shriner's Hospital in Greenville, South Carolina, in a period from 1944 to 1973. The five cases of avascular necrosis of the talus occurred among the 35 patients having combined medial and lateral releases. These five clubfeet were recurrent and recalcitrant and required extensive soft tissue release to achieve sustained correction. It is recommended that dissection of the subtalar joint, except at the talonavicular joint, be avoided and that extensive lateral ankle dissection, especially when the sinus tarsi is included, should not be performed simultaneously with the medial release unless absolutely necessary to achieve the desired anatomic relationship.", "PMID": 959868}
{"id": "pubmed23n0032_3668", "title": "Depression among the aged.", "content": "Depression in the elderly is a public health problem that will increase in magnitude as a progressively larger proportion of our population consists of those over the age of 65. Epidemiologic studies indicate that the elderly are in need of physical and mental health care. New data from 1,645 respondents in a Southeastern county show the high frequency of depressive symptomatology in the elderly and a significant relationship between poverty and depressive symptomatology. Predominant symptoms were lowered spirits, feeling helpless, a gloomy outlook on the future, a sense of powerlessness, and difficulties with sleep and appetite. A social psychiatric orientation is needed to supplement the more traditional psychodynamic formulations of depression in the aged. Adverse social conditions and cultural biases deprive the elderly person of a requisite level of self-esteem and place him at risk for depression. Treatment included humanistically based psychotherapy which can be carried out by doctors, the use of antidepressants, medications, and the development of social support systems.", "contents": "Depression among the aged. Depression in the elderly is a public health problem that will increase in magnitude as a progressively larger proportion of our population consists of those over the age of 65. Epidemiologic studies indicate that the elderly are in need of physical and mental health care. New data from 1,645 respondents in a Southeastern county show the high frequency of depressive symptomatology in the elderly and a significant relationship between poverty and depressive symptomatology. Predominant symptoms were lowered spirits, feeling helpless, a gloomy outlook on the future, a sense of powerlessness, and difficulties with sleep and appetite. A social psychiatric orientation is needed to supplement the more traditional psychodynamic formulations of depression in the aged. Adverse social conditions and cultural biases deprive the elderly person of a requisite level of self-esteem and place him at risk for depression. Treatment included humanistically based psychotherapy which can be carried out by doctors, the use of antidepressants, medications, and the development of social support systems.", "PMID": 959869}
{"id": "pubmed23n0032_3669", "title": "Rubella: pattern of outbreak in a university.", "content": "During the spring term of 1975, rubella was diagnosed in 23 students at Georgetown University in Washington, DC. The cases were concentrated among students from the School of Foreign Service and their close contacts; outbreaks did not appear in other divisions of the university. Rubella immunization of susceptible female college personnel is recommended.", "contents": "Rubella: pattern of outbreak in a university. During the spring term of 1975, rubella was diagnosed in 23 students at Georgetown University in Washington, DC. The cases were concentrated among students from the School of Foreign Service and their close contacts; outbreaks did not appear in other divisions of the university. Rubella immunization of susceptible female college personnel is recommended.", "PMID": 959870}
{"id": "pubmed23n0032_3670", "title": "Abdominal thermography in infantile and childhood liver disease.", "content": "Thermography provides an indication of the infrared energy emitted from various skin surfaces and, usually, reflects heat conveyed there from underlying organs. We have used abdominal thermography to study 62 infants and children with acute and chronic liver disease. Thirty-four healthy infants and children were studied as controls. Thermograms were abnormal in 96% of all patients with liver disease. The most common pattern was diffuse heat emission over the liver area, either alone or in combination with vascular and/or mottled patterns. A mottled pattern was more common in chronic liver disease. Although follow-up thermograms were obtained in 28 patients, the data obtained for different types of liver disease are as yet insufficient to warrant conclusions. However, it appeared that reversal of an abnormal thermogram to normal was associated with improvement of liver function and histologic findings in two patients with acute viral hepatitis. On the other hand, persistence of abnormal thermographic records was always associated with persistent hepatic disease as determined by histology and tests of liver function.", "contents": "Abdominal thermography in infantile and childhood liver disease. Thermography provides an indication of the infrared energy emitted from various skin surfaces and, usually, reflects heat conveyed there from underlying organs. We have used abdominal thermography to study 62 infants and children with acute and chronic liver disease. Thirty-four healthy infants and children were studied as controls. Thermograms were abnormal in 96% of all patients with liver disease. The most common pattern was diffuse heat emission over the liver area, either alone or in combination with vascular and/or mottled patterns. A mottled pattern was more common in chronic liver disease. Although follow-up thermograms were obtained in 28 patients, the data obtained for different types of liver disease are as yet insufficient to warrant conclusions. However, it appeared that reversal of an abnormal thermogram to normal was associated with improvement of liver function and histologic findings in two patients with acute viral hepatitis. On the other hand, persistence of abnormal thermographic records was always associated with persistent hepatic disease as determined by histology and tests of liver function.", "PMID": 959871}
{"id": "pubmed23n0032_3671", "title": "Clinical trials of tonsillectomy and adenoidectomy: limitations of existing studies and a current effort to evaluate efficacy.", "content": "Indications for tonsillectomy and adenoidectomy (T&A) are both uncertain and controversial. Only five prospective clinical trials of T&A have been reported. These studies provided important data, but failed to test the efficacy of the procedures in children most affected by tonsil- and adenoid-related conditions. A study currently under way focuses on just such children and also explores related immunologic issues.", "contents": "Clinical trials of tonsillectomy and adenoidectomy: limitations of existing studies and a current effort to evaluate efficacy. Indications for tonsillectomy and adenoidectomy (T&A) are both uncertain and controversial. Only five prospective clinical trials of T&A have been reported. These studies provided important data, but failed to test the efficacy of the procedures in children most affected by tonsil- and adenoid-related conditions. A study currently under way focuses on just such children and also explores related immunologic issues.", "PMID": 959872}
{"id": "pubmed23n0032_3672", "title": "Ostium primum atrial septal defect in the adult.", "content": "Seven adults with ostium primum atrial septal defect (ASD) are presented. All but one patient were symptomatic at the time of diagnosis with the average age being 41 years. At catheterization each had a large, low ASD, left-to-right shunt, and a cleft mitral valve leaflet with minimal regurgitation. Pulmonary hypertension occurred in one. Six patients are asymptomatic an average of 3.5 years after surgical correction. One patient died of other causes at 18 months. Rapid decompensation secondary to arrhythmias or atrioventricular block occurs much more frequently with primum than with secundum ASDs, approaching 80% by age 45. Follow-up in this series suggests that surgical correction may prevent these developments.", "contents": "Ostium primum atrial septal defect in the adult. Seven adults with ostium primum atrial septal defect (ASD) are presented. All but one patient were symptomatic at the time of diagnosis with the average age being 41 years. At catheterization each had a large, low ASD, left-to-right shunt, and a cleft mitral valve leaflet with minimal regurgitation. Pulmonary hypertension occurred in one. Six patients are asymptomatic an average of 3.5 years after surgical correction. One patient died of other causes at 18 months. Rapid decompensation secondary to arrhythmias or atrioventricular block occurs much more frequently with primum than with secundum ASDs, approaching 80% by age 45. Follow-up in this series suggests that surgical correction may prevent these developments.", "PMID": 959873}
{"id": "pubmed23n0032_3673", "title": "Tuberculous meningitis in children before and since isoniazid.", "content": "The use of isoniazid (INH) in persons exposed to active cases of tuberculosis has been challenged, Reports of toxic reactions to isoniazid warrant a review of the drug's potential benefits and hazards. This prompted a retrospective survey of cases admitted to a large county hospital to determine admission incidence before and since initiation of a program of case-finding and INH chemoprophylaxis for children in 1953. During the 24-year period surveyed (1950-1973), the population of the county increased twofold while the yearly admission rate for tuberculous meningitis in children fell from a high of eight cases to a low of two. This dramatic drop in cases admitted, coupled with an absence of any toxic reactions to the drug, provides support for continuation of the program of case-finding and isoniazid chemoprophylaxis.", "contents": "Tuberculous meningitis in children before and since isoniazid. The use of isoniazid (INH) in persons exposed to active cases of tuberculosis has been challenged, Reports of toxic reactions to isoniazid warrant a review of the drug's potential benefits and hazards. This prompted a retrospective survey of cases admitted to a large county hospital to determine admission incidence before and since initiation of a program of case-finding and INH chemoprophylaxis for children in 1953. During the 24-year period surveyed (1950-1973), the population of the county increased twofold while the yearly admission rate for tuberculous meningitis in children fell from a high of eight cases to a low of two. This dramatic drop in cases admitted, coupled with an absence of any toxic reactions to the drug, provides support for continuation of the program of case-finding and isoniazid chemoprophylaxis.", "PMID": 959874}
{"id": "pubmed23n0032_3674", "title": "Perforating ocular shotgun injuries: relationship of ocular findings to pellet ballistics.", "content": "A retrospective study of 20 cases of perforating ocular injuries due to shotgun pellets revealed visual acuity of hand motions or worse in 85%. Massive persistent vitreous hemorrhage was present in 75%. Double perforation of the globe was present in 60% of the involved eyes. Product safety measures are recommended.", "contents": "Perforating ocular shotgun injuries: relationship of ocular findings to pellet ballistics. A retrospective study of 20 cases of perforating ocular injuries due to shotgun pellets revealed visual acuity of hand motions or worse in 85%. Massive persistent vitreous hemorrhage was present in 75%. Double perforation of the globe was present in 60% of the involved eyes. Product safety measures are recommended.", "PMID": 959875}
{"id": "pubmed23n0032_3675", "title": "Current management of digitalis intoxication.", "content": "Of the commonly used drugs, digitalis has one of the highest therapeutic-toxic ratios. Mortality is significantly increased in the 20% to 30% of patients on digitalis who are in a toxic state when admitted to the hospital. Successful use of digitalis depends on avoidance of its use in situations likely to result in toxicity; recognition of the signs, symptoms, and electrocardiographic findings of digitalis intoxication; and stopping digitalis completely as the initial treatment of digitalis intoxication.", "contents": "Current management of digitalis intoxication. Of the commonly used drugs, digitalis has one of the highest therapeutic-toxic ratios. Mortality is significantly increased in the 20% to 30% of patients on digitalis who are in a toxic state when admitted to the hospital. Successful use of digitalis depends on avoidance of its use in situations likely to result in toxicity; recognition of the signs, symptoms, and electrocardiographic findings of digitalis intoxication; and stopping digitalis completely as the initial treatment of digitalis intoxication.", "PMID": 959877}
{"id": "pubmed23n0032_3676", "title": "The diagnosis and treatment of leprosy.", "content": "Leprosy is a complex disease, but recent research and the Ridley-Jopling classification which emphasize its immunologic aspects have greatly aided our understanding of and approach to the problem. The diagnosis should be considered whenever skin lesions and sensory loss occur. Dapsone remains the treatment of choice, but several newer drugs show great promise, especially in those cases whose bacilli have become sulfone resistant. Immunotherapy may play an increasingly prominent role in the future. Reactive episodes continue to be a serious complication, but the availability of thalidomide to control erythema nodosum leprosum has markedly improved the prognosis. Physicians of the US Public Health Service Hospital at Carville, Louisiana, are available at all times for consultation on these and other matters related to leprosy.", "contents": "The diagnosis and treatment of leprosy. Leprosy is a complex disease, but recent research and the Ridley-Jopling classification which emphasize its immunologic aspects have greatly aided our understanding of and approach to the problem. The diagnosis should be considered whenever skin lesions and sensory loss occur. Dapsone remains the treatment of choice, but several newer drugs show great promise, especially in those cases whose bacilli have become sulfone resistant. Immunotherapy may play an increasingly prominent role in the future. Reactive episodes continue to be a serious complication, but the availability of thalidomide to control erythema nodosum leprosum has markedly improved the prognosis. Physicians of the US Public Health Service Hospital at Carville, Louisiana, are available at all times for consultation on these and other matters related to leprosy.", "PMID": 959887}
{"id": "pubmed23n0032_3677", "title": "A direct procedure for the micro-scale preparation of acyl derivatives of nucleophilic drugs from carboxylic acids for gc separation and identification.", "content": "Free carboxylic acids were converted into mixed anhydrides suitable for the acylation of nucleophilic drugs in a one-step procedure. By preparing more than one acyl derivative and comparing the retention times of the derivatives with those of authentic drug samples with reference to suitable internal standards, the positive identification of unknown drugs was possible. The method is suitable for use in conjunction with the microphase extraction of drugs.", "contents": "A direct procedure for the micro-scale preparation of acyl derivatives of nucleophilic drugs from carboxylic acids for gc separation and identification. Free carboxylic acids were converted into mixed anhydrides suitable for the acylation of nucleophilic drugs in a one-step procedure. By preparing more than one acyl derivative and comparing the retention times of the derivatives with those of authentic drug samples with reference to suitable internal standards, the positive identification of unknown drugs was possible. The method is suitable for use in conjunction with the microphase extraction of drugs.", "PMID": 959909}
{"id": "pubmed23n0032_3678", "title": "A scanning electron microscope study of the developing rat secondary palate.", "content": "The surfaces of the developing secondary palatal shelves and palate, in Wistar strain albino rats, were examined using scanning electron microscopy. Beginning on the 16th day after insemination there was a progressive degeneration of the cell surfaces on the medial aspect of the shelves. This took the form of a loss of surface microvilli and intercellular boundaries accompanied by the appearance of clefts between the cells. Fusion of the palate was complete by the 18th day and two epithelial zones could be recognized in the soft palate. The cell surface degeneration is possibly an essential change prior to shelf fusion.", "contents": "A scanning electron microscope study of the developing rat secondary palate. The surfaces of the developing secondary palatal shelves and palate, in Wistar strain albino rats, were examined using scanning electron microscopy. Beginning on the 16th day after insemination there was a progressive degeneration of the cell surfaces on the medial aspect of the shelves. This took the form of a loss of surface microvilli and intercellular boundaries accompanied by the appearance of clefts between the cells. Fusion of the palate was complete by the 18th day and two epithelial zones could be recognized in the soft palate. The cell surface degeneration is possibly an essential change prior to shelf fusion.", "PMID": 959910}
{"id": "pubmed23n0032_3679", "title": "Efficacy of cryotherapy in retinoblastoma.", "content": "Five cases of retinoblastoma treated be freezing are reported. From these cases it appears that a single freeze-thaw cycle will destroy all tumour and that with suitable instruments it is possible to destroy tumours involving up to 30% of the volume of the globe without causing blindness.", "contents": "Efficacy of cryotherapy in retinoblastoma. Five cases of retinoblastoma treated be freezing are reported. From these cases it appears that a single freeze-thaw cycle will destroy all tumour and that with suitable instruments it is possible to destroy tumours involving up to 30% of the volume of the globe without causing blindness.", "PMID": 959914}
{"id": "pubmed23n0032_3680", "title": "Physicians and sex therapy.", "content": "The physician's role in sex therapy is discussed. Problems of sexual dysfunction and guidelines for physicians who wish to engage in sex therapy are set out briefly.", "contents": "Physicians and sex therapy. The physician's role in sex therapy is discussed. Problems of sexual dysfunction and guidelines for physicians who wish to engage in sex therapy are set out briefly.", "PMID": 959915}
{"id": "pubmed23n0032_3681", "title": "Herb use and necrodegenerative hepatitis.", "content": "Twelve patients with herbally-induced hepatitis are described and the clinicopathological features of their illness, which seem to present a recognisable spectrum, are discussed. The nature and the severity of the histological changes seem to correlate with the clinical manifestations and the immediate prognosis. Laboratory tests, especially liver function studies, are of limited diagnostic and prognostic value. The plants which contain the responsible toxins, have been identified in this country and in other parts of Southern Africa. A short list is provided of, apparently, the commonest medicinal plants in Lesotho. Many more toxic plants, however, are used in food and in diverse traditional medications. Senecio species are the principal source of hepatotoxic alkoloids, especially pyrrolizidines. Experimental studies ann evidence of similar disorders in animals, have thrown some light on the pathogenic mechanisms of these hepatotoxic and possibly hepatocarcinogenic agents. The disease in humans probably results from a combination of factors.", "contents": "Herb use and necrodegenerative hepatitis. Twelve patients with herbally-induced hepatitis are described and the clinicopathological features of their illness, which seem to present a recognisable spectrum, are discussed. The nature and the severity of the histological changes seem to correlate with the clinical manifestations and the immediate prognosis. Laboratory tests, especially liver function studies, are of limited diagnostic and prognostic value. The plants which contain the responsible toxins, have been identified in this country and in other parts of Southern Africa. A short list is provided of, apparently, the commonest medicinal plants in Lesotho. Many more toxic plants, however, are used in food and in diverse traditional medications. Senecio species are the principal source of hepatotoxic alkoloids, especially pyrrolizidines. Experimental studies ann evidence of similar disorders in animals, have thrown some light on the pathogenic mechanisms of these hepatotoxic and possibly hepatocarcinogenic agents. The disease in humans probably results from a combination of factors.", "PMID": 959916}
{"id": "pubmed23n0032_3682", "title": "Safety restraints for children travelling in motor vehicles.", "content": "The parents of 202 children were questioned about their use of safety restraints for their children while they were passengers in a motor vehicle. The result of the survey shows a low incidence of use of safety restraints by both parents and their children and that the type used for children was, in the majority of cases, both inadequate and dangerous. There was a correlation between the use of safety restraints by both parent and child on the day of questioning, but no association was found between the use of these restraints and the social class, educational standard achieved, income group, home language or home province of the parents. Neither was there any association between the use of safety restraints and a history of previous traffic accidents which had involved the family or close friends. In South Africa, the number of deaths in childhood as a result of motor vehicle accidents exceed those from the same cause in the United Kingdom. We consider that the medical profession in this country should inform the public of the desirability of fitting and using safety restraints of the correct type for children while they are passengers in motor vehicles. Reasons for the high incidence of serious injuries to children who are passengers in motor vehicles are reviewed and the methods of restraining children of different ages are discussed.", "contents": "Safety restraints for children travelling in motor vehicles. The parents of 202 children were questioned about their use of safety restraints for their children while they were passengers in a motor vehicle. The result of the survey shows a low incidence of use of safety restraints by both parents and their children and that the type used for children was, in the majority of cases, both inadequate and dangerous. There was a correlation between the use of safety restraints by both parent and child on the day of questioning, but no association was found between the use of these restraints and the social class, educational standard achieved, income group, home language or home province of the parents. Neither was there any association between the use of safety restraints and a history of previous traffic accidents which had involved the family or close friends. In South Africa, the number of deaths in childhood as a result of motor vehicle accidents exceed those from the same cause in the United Kingdom. We consider that the medical profession in this country should inform the public of the desirability of fitting and using safety restraints of the correct type for children while they are passengers in motor vehicles. Reasons for the high incidence of serious injuries to children who are passengers in motor vehicles are reviewed and the methods of restraining children of different ages are discussed.", "PMID": 959917}
{"id": "pubmed23n0032_3683", "title": "Computerised axial tomography.", "content": "Computerised axial tomography has made an impressive impact on surgical neurology, and when the body scanner becomes available it will no doubt have similar advantages for the other specialities. It is a safe non-invasive method of investigating pathological processes of the brain. The amount of information so obtained is accurate to within 1% and at least a hundred times more inforation is obtained than from ordinary X-ray investigations. A review of 1 000 patients scanned is presented. Computerised axial tomography, if artefacts are eliminated completely, is most unlikely to yield a false positive scan. Its greatest value, however, is in the diagnosis of lesions in patients with head injuries and abscesses because these patients are very often moribund, and angiography, even when done expertly, may aggravate the clinical condition. Orbital and retro-orbital tumours have proved to be difficult to display other than by the EMI scanner methods. Acoustic neurinomata and meningiomata are also displayed with clarity, but owing to 'overshoot' artefact, other lesions, which do not enhance to the same extent on injection of Conray or Urografin, still offer a diagnostic problem. The investigation of epileptic patients has proved disappointing for the same reason that artefact obscures the low temporal lobe cut.", "contents": "Computerised axial tomography. Computerised axial tomography has made an impressive impact on surgical neurology, and when the body scanner becomes available it will no doubt have similar advantages for the other specialities. It is a safe non-invasive method of investigating pathological processes of the brain. The amount of information so obtained is accurate to within 1% and at least a hundred times more inforation is obtained than from ordinary X-ray investigations. A review of 1 000 patients scanned is presented. Computerised axial tomography, if artefacts are eliminated completely, is most unlikely to yield a false positive scan. Its greatest value, however, is in the diagnosis of lesions in patients with head injuries and abscesses because these patients are very often moribund, and angiography, even when done expertly, may aggravate the clinical condition. Orbital and retro-orbital tumours have proved to be difficult to display other than by the EMI scanner methods. Acoustic neurinomata and meningiomata are also displayed with clarity, but owing to 'overshoot' artefact, other lesions, which do not enhance to the same extent on injection of Conray or Urografin, still offer a diagnostic problem. The investigation of epileptic patients has proved disappointing for the same reason that artefact obscures the low temporal lobe cut.", "PMID": 959918}
{"id": "pubmed23n0032_3684", "title": "Ageing in a plural society.", "content": "The differential position and involvement of the aged in each population group in the Republic of South Africa are illustrated on the basis of an analysis of the age structure of the population, sex ratios, life expectancy, degree and nature of urbanisation and industrialisation and family structure. It is concluded that the problems and needs of the aged in modernising plural societies differ for each population group, and that there thus exists a need for a flexible and non-dogmatic approach to the needs of the aged.", "contents": "Ageing in a plural society. The differential position and involvement of the aged in each population group in the Republic of South Africa are illustrated on the basis of an analysis of the age structure of the population, sex ratios, life expectancy, degree and nature of urbanisation and industrialisation and family structure. It is concluded that the problems and needs of the aged in modernising plural societies differ for each population group, and that there thus exists a need for a flexible and non-dogmatic approach to the needs of the aged.", "PMID": 959919}
{"id": "pubmed23n0032_3685", "title": "Genetic disorders in Southern Africa.", "content": "Certain uncommon genetic disorders occur relatively frequently in the various population groups of Southern Africa. Prominent among these are porphyria, colonic polyposis and sclerosteosis in the Afrikaner community, Huntington's chorea in the British, Gaucher's and Tay-Sachs diseases in the Jewish population, glucose-6-phosphate dehydrogenase deficiency (G-6-PD deficiency) and thalassaemia in the Greek community, various skeletal dysplasias in the Black group, lipoid proteinosis and cleidocranial dysostosis in the Cape Coloured population, diabetes mellitus in the Indian community and retinitis pigmentosa in the Tristan da Cunha islanders. In addition, 'private' syndromes have been encountered in virtually every group. Awareness of the ethnic distribution of unusual genetic conditions is of considerable practical importance during the differential diagnosis of obscure disease.", "contents": "Genetic disorders in Southern Africa. Certain uncommon genetic disorders occur relatively frequently in the various population groups of Southern Africa. Prominent among these are porphyria, colonic polyposis and sclerosteosis in the Afrikaner community, Huntington's chorea in the British, Gaucher's and Tay-Sachs diseases in the Jewish population, glucose-6-phosphate dehydrogenase deficiency (G-6-PD deficiency) and thalassaemia in the Greek community, various skeletal dysplasias in the Black group, lipoid proteinosis and cleidocranial dysostosis in the Cape Coloured population, diabetes mellitus in the Indian community and retinitis pigmentosa in the Tristan da Cunha islanders. In addition, 'private' syndromes have been encountered in virtually every group. Awareness of the ethnic distribution of unusual genetic conditions is of considerable practical importance during the differential diagnosis of obscure disease.", "PMID": 959924}
{"id": "pubmed23n0032_3686", "title": "Isoniazid acetylator status of Black South African tuberculosis patients.", "content": "Black male tuberculosis patients (106) were phenotyped into 59% fast and 41% slow acetylators with isoniazid (NH) plasma half-lives. The antimode time dividing the two acetylator groups was longer than that previously reported for Whites. The therapeutic significance of INH acetylator phenotyping per se is questioned since it provides neither a measurement of the amount of INH absorption nor of its distribution within the body.", "contents": "Isoniazid acetylator status of Black South African tuberculosis patients. Black male tuberculosis patients (106) were phenotyped into 59% fast and 41% slow acetylators with isoniazid (NH) plasma half-lives. The antimode time dividing the two acetylator groups was longer than that previously reported for Whites. The therapeutic significance of INH acetylator phenotyping per se is questioned since it provides neither a measurement of the amount of INH absorption nor of its distribution within the body.", "PMID": 959925}
{"id": "pubmed23n0032_3687", "title": "Haemodynamics and treatment of right ventricular infarction.", "content": "In this article 2 cases of myocardial infarction with haemodynamically dominant right ventricular infarction are reported. The incidence and significance of the condition are discussed. The use of haemodynamic monitoring in diagnosis and management is stressed and the response to fluid therapy, either alone or together with inotropic drugs, is discussed.", "contents": "Haemodynamics and treatment of right ventricular infarction. In this article 2 cases of myocardial infarction with haemodynamically dominant right ventricular infarction are reported. The incidence and significance of the condition are discussed. The use of haemodynamic monitoring in diagnosis and management is stressed and the response to fluid therapy, either alone or together with inotropic drugs, is discussed.", "PMID": 959926}
{"id": "pubmed23n0032_3688", "title": "Poisoning associated with witchdoctor attendance.", "content": "Eight patients with poisoning associated with treatment by a witchdoctor are presented. Six patients died. A therapeutic approach to the problem is suggested.", "contents": "Poisoning associated with witchdoctor attendance. Eight patients with poisoning associated with treatment by a witchdoctor are presented. Six patients died. A therapeutic approach to the problem is suggested.", "PMID": 959927}
{"id": "pubmed23n0032_3689", "title": "The effect of diazepam on human pancreatic secretion.", "content": "The effect of intravenous and intramuscular administration of diazepam on secretin- and cholecystokinin-stimulated pancreatic secretion was studied in man. Diazepam was not found to have a significant effect on mean volume out-put, bicarbonate and enzyme concentrations and colour index. It was acceptable as premedication for routine pancreatic function studies.", "contents": "The effect of diazepam on human pancreatic secretion. The effect of intravenous and intramuscular administration of diazepam on secretin- and cholecystokinin-stimulated pancreatic secretion was studied in man. Diazepam was not found to have a significant effect on mean volume out-put, bicarbonate and enzyme concentrations and colour index. It was acceptable as premedication for routine pancreatic function studies.", "PMID": 959928}
{"id": "pubmed23n0032_3690", "title": "Measuring emotional stress in Africa: the difficulties involved and some results.", "content": "Two surveys of emotional stress were undertaken and the difficulties experienced in each survey are discussed. In the first survey, 3 sets of personality inventories, selected to identify the type A personality that is associated with the early onset of coronary heart disease, were completed by 95 men, aged from 40 to 50 years, who came from 4 groups: White Rhodesians, urban Blacks of high and low social status, and Blacks from a rural area. The same trend was shown by all 3 inventories, which indicates that type A behaviour is least common among Whites, becomes progressively more marked in Blacks of upper social class, then in Blacks of lower social status, and is greatest in Blacks living in a rural environment. The psychogalvanic response to emotional stress (fear) was used to assess autonomic lability in the first 3 groups.", "contents": "Measuring emotional stress in Africa: the difficulties involved and some results. Two surveys of emotional stress were undertaken and the difficulties experienced in each survey are discussed. In the first survey, 3 sets of personality inventories, selected to identify the type A personality that is associated with the early onset of coronary heart disease, were completed by 95 men, aged from 40 to 50 years, who came from 4 groups: White Rhodesians, urban Blacks of high and low social status, and Blacks from a rural area. The same trend was shown by all 3 inventories, which indicates that type A behaviour is least common among Whites, becomes progressively more marked in Blacks of upper social class, then in Blacks of lower social status, and is greatest in Blacks living in a rural environment. The psychogalvanic response to emotional stress (fear) was used to assess autonomic lability in the first 3 groups.", "PMID": 959929}
{"id": "pubmed23n0032_3691", "title": "Coagulation and fibrinolytic properties of peripheral venous blood in chronic ectopic pregnancy.", "content": "The coagulation and fibrinolytic properties of peripheral venous blood was studied in 10 Black patients with chronic ectopic pregnancy. There were significant elevations of plasma fibrinogen and serum fibrin-fibrinogen degradation products (FDP) and prolongation of the euglobulin lysis time. These features are believed to be compatible with chronic inflammation. No evidence of a haemostatic defect was found.", "contents": "Coagulation and fibrinolytic properties of peripheral venous blood in chronic ectopic pregnancy. The coagulation and fibrinolytic properties of peripheral venous blood was studied in 10 Black patients with chronic ectopic pregnancy. There were significant elevations of plasma fibrinogen and serum fibrin-fibrinogen degradation products (FDP) and prolongation of the euglobulin lysis time. These features are believed to be compatible with chronic inflammation. No evidence of a haemostatic defect was found.", "PMID": 959930}
{"id": "pubmed23n0032_3692", "title": "Geriatric care: where does the responsibility lie?", "content": "In contrast to purposeful efforts in South Africa to lay sound foundations for the development of social services for the aged, little constructive planning has been done by medical authorities to provide for the special health needs of the elderly on a national basis and based on recognised geriatric principles.", "contents": "Geriatric care: where does the responsibility lie? In contrast to purposeful efforts in South Africa to lay sound foundations for the development of social services for the aged, little constructive planning has been done by medical authorities to provide for the special health needs of the elderly on a national basis and based on recognised geriatric principles.", "PMID": 959931}
{"id": "pubmed23n0032_3693", "title": "Hypertension detected in young black women by routine screening in a family planning clinics.", "content": "The prevalence of hypertension, defined as a diastolic blood pressure over 100 mmHg, among young Black women who attended a family planning clinic in Johannesburg, was 2,85%, while in those over 35 years of age it was 8,5%. There was no correlation between hypertension and urbanisation, occupation or urinary sodium output. Within 6 months of diagnoisis, 84% of the women with hypertension had defaulted from the treatment clinic. Family planning clinics can economically screen patients for hypertension, but in this community a separate screening programme would not succeed until patient compliance has improved.", "contents": "Hypertension detected in young black women by routine screening in a family planning clinics. The prevalence of hypertension, defined as a diastolic blood pressure over 100 mmHg, among young Black women who attended a family planning clinic in Johannesburg, was 2,85%, while in those over 35 years of age it was 8,5%. There was no correlation between hypertension and urbanisation, occupation or urinary sodium output. Within 6 months of diagnoisis, 84% of the women with hypertension had defaulted from the treatment clinic. Family planning clinics can economically screen patients for hypertension, but in this community a separate screening programme would not succeed until patient compliance has improved.", "PMID": 959934}
{"id": "pubmed23n0032_3694", "title": "[Cycling, fitness and the prevention of ischemic heart disease].", "content": "A short review of the prevalence of ischaemic heart disease in South African Whites is given, and primary prevention, which entails an alteration in life style, is emphasised. The role of increased physical activity in primary prevention is discussed. Any programme to increase the fitness status of a population based on activities that must be performed outside the daily routine, willhave limited impact. The possible role of cycling is discussed and it is shown that ordinary cycling to work probably causes sufficient stimulation of the cardiovascular system to produce an acceptable standard of physical fitness.", "contents": "[Cycling, fitness and the prevention of ischemic heart disease]. A short review of the prevalence of ischaemic heart disease in South African Whites is given, and primary prevention, which entails an alteration in life style, is emphasised. The role of increased physical activity in primary prevention is discussed. Any programme to increase the fitness status of a population based on activities that must be performed outside the daily routine, willhave limited impact. The possible role of cycling is discussed and it is shown that ordinary cycling to work probably causes sufficient stimulation of the cardiovascular system to produce an acceptable standard of physical fitness.", "PMID": 959935}
{"id": "pubmed23n0032_3695", "title": "Pulsus paradoxus in the bedside assessment of patients with bronchial asthma.", "content": "Pulsus paradoxus (PP) was measured in 35 patients with bronchial asthma. The presence and degree of PP correlated well with the clinical conditions of the patient and with peak expiratory flow rate (PEF). PP and PEF are useful bedside measurements in the assessment and management of patients with bronchial asthma. Although the degree of PP is usually directly related to the severity of the attack of asthma, it was absent in the one patient who remained severly ill with unreleived bronchospam when he became exhausted. Our experience with this patient, and with previous patients who are not included in the present analysis, emphasises the fact that PP must not be considered in isolation but in conjunction with the patient's clinical state and with other indices of the severity of the astmatic attack.", "contents": "Pulsus paradoxus in the bedside assessment of patients with bronchial asthma. Pulsus paradoxus (PP) was measured in 35 patients with bronchial asthma. The presence and degree of PP correlated well with the clinical conditions of the patient and with peak expiratory flow rate (PEF). PP and PEF are useful bedside measurements in the assessment and management of patients with bronchial asthma. Although the degree of PP is usually directly related to the severity of the attack of asthma, it was absent in the one patient who remained severly ill with unreleived bronchospam when he became exhausted. Our experience with this patient, and with previous patients who are not included in the present analysis, emphasises the fact that PP must not be considered in isolation but in conjunction with the patient's clinical state and with other indices of the severity of the astmatic attack.", "PMID": 959936}
{"id": "pubmed23n0032_3696", "title": "An interracial comparison of insulin secretion in normal non-pregnant women.", "content": "The plasma insulin response to oral glucose in normal, non-pregnant, premenopausal Indian, Black, andWhite women was investigated. Indian women responded by an excessive secretion of insulin relative to the other two groups. This primarily affected the late phase of insulin release.", "contents": "An interracial comparison of insulin secretion in normal non-pregnant women. The plasma insulin response to oral glucose in normal, non-pregnant, premenopausal Indian, Black, andWhite women was investigated. Indian women responded by an excessive secretion of insulin relative to the other two groups. This primarily affected the late phase of insulin release.", "PMID": 959937}
{"id": "pubmed23n0032_3697", "title": "Geriatric medicine - the challenge of the 1970s.", "content": "The rising number of elderly people in the community poses a great challenge to the medical and allied professions. This article outlines the urgent need for the planning and provision of comprehensive services for the elderly. The importance of health surveillance, a preventive approach and the health team work is stressed. The need for the introduction of geriatrics into undergraduate and postgraduate medical education is emphasised.", "contents": "Geriatric medicine - the challenge of the 1970s. The rising number of elderly people in the community poses a great challenge to the medical and allied professions. This article outlines the urgent need for the planning and provision of comprehensive services for the elderly. The importance of health surveillance, a preventive approach and the health team work is stressed. The need for the introduction of geriatrics into undergraduate and postgraduate medical education is emphasised.", "PMID": 959938}
{"id": "pubmed23n0032_3698", "title": "Subcutaneous phycomycosis from Pondicherry, South India.", "content": "Four cases of subcutaneous phycomycosis diagnosed by isolating Basidiobolus meristosorus from affected tissue, are reported along with the growth characters and morphological features of this fungus. This is the first report from Pondicherry, South India where it was it was possible to isolate the causative fungus from each case.", "contents": "Subcutaneous phycomycosis from Pondicherry, South India. Four cases of subcutaneous phycomycosis diagnosed by isolating Basidiobolus meristosorus from affected tissue, are reported along with the growth characters and morphological features of this fungus. This is the first report from Pondicherry, South India where it was it was possible to isolate the causative fungus from each case.", "PMID": 959942}
{"id": "pubmed23n0032_3699", "title": "Sulphitolysis in keratinolysi. Biochemical proof.", "content": "The presence of S-sulphocysteine in filtrates of the dermatophyte Keratinomyces ajelloi growing on human hair in a culture medium buffered by pH 7-4 by phosphates was demonstrated by means of ion exchange chromatography techniques. S-sulphocysteine being destroyed during acidic hydrolysis was identified after enzymic hyrolysis of dialyzed and lyophilized filtrates. This result indicates that sulphitolysis occurs during kerationlysis performed by K. ajelloi. As thiosulphuric esters were shown present in hair perforations made by Microsporum gypseum, we think sulphitolysis is a common mechanism developed by dermatophytes to attack keratin.", "contents": "Sulphitolysis in keratinolysi. Biochemical proof. The presence of S-sulphocysteine in filtrates of the dermatophyte Keratinomyces ajelloi growing on human hair in a culture medium buffered by pH 7-4 by phosphates was demonstrated by means of ion exchange chromatography techniques. S-sulphocysteine being destroyed during acidic hydrolysis was identified after enzymic hyrolysis of dialyzed and lyophilized filtrates. This result indicates that sulphitolysis occurs during kerationlysis performed by K. ajelloi. As thiosulphuric esters were shown present in hair perforations made by Microsporum gypseum, we think sulphitolysis is a common mechanism developed by dermatophytes to attack keratin.", "PMID": 959943}
{"id": "pubmed23n0032_3700", "title": "A cytological study of morphogenesis in Coccidioides immitis.", "content": "Coccidioides immitis is still classified as a Deuteromycete because the perfect stage is unknown. Mating experiments have been unsuccessful. Some investigators consider the endosporulating spherule to be a sporangium with sporangiospores, but others describe it as an ascus. The development of a reproducible method for cultivation of the spherule-endospore cycle in vitro provided an opportunity to study cytological changes occuring throughout the arthrospore-hyphae-arthrospore cycle and the arthrospore-spherule-endospore cycle. Our observations of the arthrospore-mycelial cycle confirm an earlier report by another investigator demonstrating a resting stage followed by ring formation, a V-form, and a filament stage. In addition, we describe four pairs of chromosomes and formation of possible spindles and nucleoli. The spherule-endospore cycle is initiated by an arthrospore with a single large nucleus, most probably derived from the multinucleate arthrospores in the inoculum. This uninucleate arthrospore is present for less than 24 hours. During continued incubation, the arthrospore becomes a round cell with four pairs of chromosomes. Subsquently, the cell enlarges to become a spherule and this growth is accompanied by a series of synchronous divisions of the nuclei. Although endospores are formed apparently progressive cleavage, clusters of uninucleate endospores are enclosed in a membranous sac as seen in squashed spherule preparations. The question of a relationship to the phycomycetes or the ascomycetes remains unresolved.", "contents": "A cytological study of morphogenesis in Coccidioides immitis. Coccidioides immitis is still classified as a Deuteromycete because the perfect stage is unknown. Mating experiments have been unsuccessful. Some investigators consider the endosporulating spherule to be a sporangium with sporangiospores, but others describe it as an ascus. The development of a reproducible method for cultivation of the spherule-endospore cycle in vitro provided an opportunity to study cytological changes occuring throughout the arthrospore-hyphae-arthrospore cycle and the arthrospore-spherule-endospore cycle. Our observations of the arthrospore-mycelial cycle confirm an earlier report by another investigator demonstrating a resting stage followed by ring formation, a V-form, and a filament stage. In addition, we describe four pairs of chromosomes and formation of possible spindles and nucleoli. The spherule-endospore cycle is initiated by an arthrospore with a single large nucleus, most probably derived from the multinucleate arthrospores in the inoculum. This uninucleate arthrospore is present for less than 24 hours. During continued incubation, the arthrospore becomes a round cell with four pairs of chromosomes. Subsquently, the cell enlarges to become a spherule and this growth is accompanied by a series of synchronous divisions of the nuclei. Although endospores are formed apparently progressive cleavage, clusters of uninucleate endospores are enclosed in a membranous sac as seen in squashed spherule preparations. The question of a relationship to the phycomycetes or the ascomycetes remains unresolved.", "PMID": 959944}
{"id": "pubmed23n0032_3701", "title": "Preliminary studies on conidial liberation of Blastomyces dermatitidis and Histoplasma capsulatum.", "content": "Controlled attempts to release viable conidia from cultures of Blastomyces dermatitidis and Histoplasma capsulatum with air samplers were unsuccessful. Air velocity was not fount to be a limiting factor since the conidia remained attached after conidiophores had been violently shaken by air currents in an observation chamber. Wetting these same conidiophores readily released the conidia. It is inferred that in nature conidia may be liberated by exposure to water and then dispersed by air currents.", "contents": "Preliminary studies on conidial liberation of Blastomyces dermatitidis and Histoplasma capsulatum. Controlled attempts to release viable conidia from cultures of Blastomyces dermatitidis and Histoplasma capsulatum with air samplers were unsuccessful. Air velocity was not fount to be a limiting factor since the conidia remained attached after conidiophores had been violently shaken by air currents in an observation chamber. Wetting these same conidiophores readily released the conidia. It is inferred that in nature conidia may be liberated by exposure to water and then dispersed by air currents.", "PMID": 959945}
{"id": "pubmed23n0032_3702", "title": "A study on transformation in a mutant of Candida pseudotropicalis.", "content": "This paper reports on the possible influence of various factors on the frequency of transformation in Candida pseudtropicalis. The concentration of DNA used varied between 25 mug and 100 mug/ml. The maximum percentage transformation 0-16% occured at 75 mug/ml.", "contents": "A study on transformation in a mutant of Candida pseudotropicalis. This paper reports on the possible influence of various factors on the frequency of transformation in Candida pseudtropicalis. The concentration of DNA used varied between 25 mug and 100 mug/ml. The maximum percentage transformation 0-16% occured at 75 mug/ml.", "PMID": 959946}
{"id": "pubmed23n0032_3703", "title": "Isolation of Sporothrix schenckii in the soil in Israel in relation to a new case in man.", "content": "A new case, the third in Israel, of subcutaneous sporotrichosis is presented. A pigmentary strain of Sporothrix schenckii was cultured from the unopened and necrotic nodules of the left hand and forearm of an 80-year-old male. Experimental inoculation of the isolated fungus into mice led to the dissemination of the organism in the liver, kidneys and lungs inoculated intraperitoneally, and the development of orchitis with abscess formation in those inoculated intratesticularly. In both groups of mice the causative agent was successfully recovered. Three months of treatment with oral potassium iodide led to the disappearance of the lesions. The source of contamination was found to be the soil adhering to fragments of wood. Two strains of a fungus, morphologically identical to the human S. schenckii, were isolated through the mouse procedure and agar-plating method from soil samples collected in the vicinity of the patient's residence. The soil isolates also proved to be pathogenic in animal inoculation. This is the first isolation of S. schenckii from soils in Israel. The possible relationships between the soil isolates and Ceratocystis stenoceras are discussed.", "contents": "Isolation of Sporothrix schenckii in the soil in Israel in relation to a new case in man. A new case, the third in Israel, of subcutaneous sporotrichosis is presented. A pigmentary strain of Sporothrix schenckii was cultured from the unopened and necrotic nodules of the left hand and forearm of an 80-year-old male. Experimental inoculation of the isolated fungus into mice led to the dissemination of the organism in the liver, kidneys and lungs inoculated intraperitoneally, and the development of orchitis with abscess formation in those inoculated intratesticularly. In both groups of mice the causative agent was successfully recovered. Three months of treatment with oral potassium iodide led to the disappearance of the lesions. The source of contamination was found to be the soil adhering to fragments of wood. Two strains of a fungus, morphologically identical to the human S. schenckii, were isolated through the mouse procedure and agar-plating method from soil samples collected in the vicinity of the patient's residence. The soil isolates also proved to be pathogenic in animal inoculation. This is the first isolation of S. schenckii from soils in Israel. The possible relationships between the soil isolates and Ceratocystis stenoceras are discussed.", "PMID": 959947}
{"id": "pubmed23n0032_3704", "title": "La maladie post-operatoire: Is there order in variety? The six stimulus-response sequences.", "content": "The search for a unifying concept in all post-traumatic metabolism, symbolized by the phrase \"la maladie post-operatoire\" has been a fruitful one, not so much because of success, as through its very failure. There is such variety in convalescent patterns that it has made it essential for students of this subject to look more closely at the nature of the stimulus response patterns and bring sharply into focus those recurring phenomena whose mixture together determines the final clinical result. In this brief introductory paper six groups of stimulus-response patterns have been identified and analyzed. The clinician needs an understanding of these patterns in everything that he does for the patient, both within the operating room and on the ward. The student of surgery must make an effort to differentiate amongst the six stimulus-response patterns. If he fails to do so he will be analyzing all surgical recovery as falling under a single sequence, an error that has been all too frequent in the literature. There may be other stimulus response patterns of greater importance which will be added to the list presented here, as research in the coming decades increases our understanding of convalescent biology. This presentation will serve as an introduction to the other papers gathered in this symposium by Dr. Clowes, all of which cast further important light on the details of the stimulus and response in surgical metabolism.", "contents": "La maladie post-operatoire: Is there order in variety? The six stimulus-response sequences. The search for a unifying concept in all post-traumatic metabolism, symbolized by the phrase \"la maladie post-operatoire\" has been a fruitful one, not so much because of success, as through its very failure. There is such variety in convalescent patterns that it has made it essential for students of this subject to look more closely at the nature of the stimulus response patterns and bring sharply into focus those recurring phenomena whose mixture together determines the final clinical result. In this brief introductory paper six groups of stimulus-response patterns have been identified and analyzed. The clinician needs an understanding of these patterns in everything that he does for the patient, both within the operating room and on the ward. The student of surgery must make an effort to differentiate amongst the six stimulus-response patterns. If he fails to do so he will be analyzing all surgical recovery as falling under a single sequence, an error that has been all too frequent in the literature. There may be other stimulus response patterns of greater importance which will be added to the list presented here, as research in the coming decades increases our understanding of convalescent biology. This presentation will serve as an introduction to the other papers gathered in this symposium by Dr. Clowes, all of which cast further important light on the details of the stimulus and response in surgical metabolism.", "PMID": 959949}
{"id": "pubmed23n0032_3705", "title": "Thrombosis and intravascular coagulation.", "content": "Stress, including trauma and sepsis, is associated with a state of hypercoagulability. In these circumstances the patient is at risk of generalized or local thrombotic complications. New laboratory investigative procedures facilitate diagnosis and permit improved assessment of therapy, which at present remains of unproven efficacy both in the general and local situation.", "contents": "Thrombosis and intravascular coagulation. Stress, including trauma and sepsis, is associated with a state of hypercoagulability. In these circumstances the patient is at risk of generalized or local thrombotic complications. New laboratory investigative procedures facilitate diagnosis and permit improved assessment of therapy, which at present remains of unproven efficacy both in the general and local situation.", "PMID": 959952}
{"id": "pubmed23n0032_3706", "title": "Anaerobic mediastinitis.", "content": "Three patients with anaerobic mediastinitis associated with retropharyngeal abscess have been encountered recently. Dental infections accounted for two instances, and the third instance resulted from rupture of the thoracic esophagus from blunt trauma. Each was marked by extreme difficulty in achieving complete abscess drainage due to the initially unrecognized virulence of the causative anaerobic bacteria. Complications were frequent and included empyema, pericarditis and aspiration pneumonia. The combination of vigorous diagnostic efforts, prompt operation and reoperation when necessary plus specific antibiotic effective against anaerobes led to a successful outcome for each patient.", "contents": "Anaerobic mediastinitis. Three patients with anaerobic mediastinitis associated with retropharyngeal abscess have been encountered recently. Dental infections accounted for two instances, and the third instance resulted from rupture of the thoracic esophagus from blunt trauma. Each was marked by extreme difficulty in achieving complete abscess drainage due to the initially unrecognized virulence of the causative anaerobic bacteria. Complications were frequent and included empyema, pericarditis and aspiration pneumonia. The combination of vigorous diagnostic efforts, prompt operation and reoperation when necessary plus specific antibiotic effective against anaerobes led to a successful outcome for each patient.", "PMID": 959954}
{"id": "pubmed23n0032_3707", "title": "Radioactive technetium phosphate bone scanning in preoperative assessment and follow-up study of patients with primary cancer of the breast.", "content": "Seventy-five female patients with early primary cancer of the breast were studied by bone scanning with 99mTc labeled phosphate at the time of initial presentation and serially during follow-up examination. Eleven patients had an abnormal bone scan at the time of presentation. During follow-up periods, which have ranged from three to 34 months with a mean of 16 months, abnormal bone scans have developed in a further 13 patients. To date, nine of the 24 patients with an abnormal bone scan have died, while only one of the 51 with a persistently normal scan has died. It is concluded that an abnormal bone scan carries a significant risk of earlier death due to metastatic disease.", "contents": "Radioactive technetium phosphate bone scanning in preoperative assessment and follow-up study of patients with primary cancer of the breast. Seventy-five female patients with early primary cancer of the breast were studied by bone scanning with 99mTc labeled phosphate at the time of initial presentation and serially during follow-up examination. Eleven patients had an abnormal bone scan at the time of presentation. During follow-up periods, which have ranged from three to 34 months with a mean of 16 months, abnormal bone scans have developed in a further 13 patients. To date, nine of the 24 patients with an abnormal bone scan have died, while only one of the 51 with a persistently normal scan has died. It is concluded that an abnormal bone scan carries a significant risk of earlier death due to metastatic disease.", "PMID": 959955}
{"id": "pubmed23n0032_3708", "title": "Needle biopsy of the thyroid.", "content": "As with any specialized diagnostic technique, the degree of success and safety of needle biopsy of the thyroid are largely based on experience. While an adequate core of tissue for examination is imperative, the interpretation by the pathologist is equally important. A close liaison of the latter with his clinical colleagues is necessary for optimal pathologic evaluation. A review of this experience during a 20 year period shows that needle biopsy is a reliable diagnostic procedure in more than 90 per cent of the patients. It is unlikely to disseminate the disease or compromise the prognosis, and it is a safe office procedure.", "contents": "Needle biopsy of the thyroid. As with any specialized diagnostic technique, the degree of success and safety of needle biopsy of the thyroid are largely based on experience. While an adequate core of tissue for examination is imperative, the interpretation by the pathologist is equally important. A close liaison of the latter with his clinical colleagues is necessary for optimal pathologic evaluation. A review of this experience during a 20 year period shows that needle biopsy is a reliable diagnostic procedure in more than 90 per cent of the patients. It is unlikely to disseminate the disease or compromise the prognosis, and it is a safe office procedure.", "PMID": 959956}
{"id": "pubmed23n0032_3709", "title": "Intravenous iron-dextran therapy in the treatment of anemia occurring in surgical, gynecologic and obstetric patients.", "content": "An infusion of iron-dextran diluted in 1,000 milliliters of physiologic saline solution was given to 51 patients. The average hemoglobin response was 1.9 grams per deciliter per week. Mean corpuscular volume, mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin deficits also were corrected. There were no allergic reactions. This is an extremely reliable and safe method of replenishing depleted iron in patients.", "contents": "Intravenous iron-dextran therapy in the treatment of anemia occurring in surgical, gynecologic and obstetric patients. An infusion of iron-dextran diluted in 1,000 milliliters of physiologic saline solution was given to 51 patients. The average hemoglobin response was 1.9 grams per deciliter per week. Mean corpuscular volume, mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin deficits also were corrected. There were no allergic reactions. This is an extremely reliable and safe method of replenishing depleted iron in patients.", "PMID": 959957}
{"id": "pubmed23n0032_3710", "title": "Risk factors in pulmonary embolism.", "content": "The influence of several diseases and conditions upon the prevalence of pulmonary embolism in autopsies performed during a ten year period at the University of Michigan has been analyzed. The major factors contributing to an increase in risk of development of pulmonary embolism include heart disease, certain types of cancer, obesity, acute paraplegia and accidental and operative trauma. These and several other risk factors defined in other studies should be used in a selective program designed to increase the rate of detection of deep venous thrombosis before pulmonary embolism occurs, or alternatively, patients at increased risk should receive prophylactic low dosage heparin therapy during hospitalization.", "contents": "Risk factors in pulmonary embolism. The influence of several diseases and conditions upon the prevalence of pulmonary embolism in autopsies performed during a ten year period at the University of Michigan has been analyzed. The major factors contributing to an increase in risk of development of pulmonary embolism include heart disease, certain types of cancer, obesity, acute paraplegia and accidental and operative trauma. These and several other risk factors defined in other studies should be used in a selective program designed to increase the rate of detection of deep venous thrombosis before pulmonary embolism occurs, or alternatively, patients at increased risk should receive prophylactic low dosage heparin therapy during hospitalization.", "PMID": 959958}
{"id": "pubmed23n0032_3711", "title": "Acute renal failure after ligation of the hepatic artery.", "content": "Acute renal failure developed in nine of 78 patients who were subjected to hepatic artery ligation for nonresectable and extensive malignant tumor of the liver. Of those nine, six had hepatomas, one cholangiocarcinoma, one metastatic islet-cell carcinoma and one metastatic melanoma. Preoperative renal function as reflected in blood-urea-nitrogen and serum creatinine values was within normal limits. There were marked elevations of serum glutamic-oxalacetic transaminase and lactic dehydrogenase levels after hepatic artery ligation, an indication of massive ischemic injury of the tumor and the liver. A diagnosis of acute renal failure was established within 14 to 70 hours after hepatic artery ligation. In five patients, oliguric renal failure developed, and in four, high urinary output renal failure. In only three patients did systemic hypotension and hypovolemia precede acute renal failure. Seven of the nine patients died. Postmortem examination was done in five patients, and in only two was there evidence of renal tubular necrosis. The factors contributing to acute renal failure appear to be extensive involvement of the liver by tumor, presence of ascites and jaundice, occlusion of the portal vein and hyperuricemia. The presence of any one of the foregoing contraindicates the procedure.", "contents": "Acute renal failure after ligation of the hepatic artery. Acute renal failure developed in nine of 78 patients who were subjected to hepatic artery ligation for nonresectable and extensive malignant tumor of the liver. Of those nine, six had hepatomas, one cholangiocarcinoma, one metastatic islet-cell carcinoma and one metastatic melanoma. Preoperative renal function as reflected in blood-urea-nitrogen and serum creatinine values was within normal limits. There were marked elevations of serum glutamic-oxalacetic transaminase and lactic dehydrogenase levels after hepatic artery ligation, an indication of massive ischemic injury of the tumor and the liver. A diagnosis of acute renal failure was established within 14 to 70 hours after hepatic artery ligation. In five patients, oliguric renal failure developed, and in four, high urinary output renal failure. In only three patients did systemic hypotension and hypovolemia precede acute renal failure. Seven of the nine patients died. Postmortem examination was done in five patients, and in only two was there evidence of renal tubular necrosis. The factors contributing to acute renal failure appear to be extensive involvement of the liver by tumor, presence of ascites and jaundice, occlusion of the portal vein and hyperuricemia. The presence of any one of the foregoing contraindicates the procedure.", "PMID": 959959}
{"id": "pubmed23n0032_3712", "title": "Evaluation of dissection of the axilla in modified radical mastectomy.", "content": "Fifty consecutive unselected patients with infiltrating carcinoma of the breast underwent radical mastectomy in two consecutive stages. The initial operation was a modified radical mastectomy, with preservation of the pectoral muscles, and the second stage, a procedure to transform the initial operation into a standard radical mastectomy. An analysis of the location and the number of the lymph nodes recovered in the two stage operation shows that a so-called modified radical mastectomy is inadequate to ensure the clearing of the axilla. Lymph nodes were recovered at the second stage of the operation in 36 patients, and seven of these patients had metastasis. Modified radical mastectomy was effective in erradicating the lymph nodes of the lower part of the axilla but inadequate for lymph nodes at levels II and III and, especially, the interpectoral lymph nodes.", "contents": "Evaluation of dissection of the axilla in modified radical mastectomy. Fifty consecutive unselected patients with infiltrating carcinoma of the breast underwent radical mastectomy in two consecutive stages. The initial operation was a modified radical mastectomy, with preservation of the pectoral muscles, and the second stage, a procedure to transform the initial operation into a standard radical mastectomy. An analysis of the location and the number of the lymph nodes recovered in the two stage operation shows that a so-called modified radical mastectomy is inadequate to ensure the clearing of the axilla. Lymph nodes were recovered at the second stage of the operation in 36 patients, and seven of these patients had metastasis. Modified radical mastectomy was effective in erradicating the lymph nodes of the lower part of the axilla but inadequate for lymph nodes at levels II and III and, especially, the interpectoral lymph nodes.", "PMID": 959960}
{"id": "pubmed23n0032_3713", "title": "Evaluation of use of the rigid dressing in amputation of the lower extremity.", "content": "Twenty-seven arteriosclerotic amputees were evaluated to determine the value of the rigid dressing technique for amputation wound management. Results were compared with those for patients having conventional stump wound management. The two groups were similar in number, age and incidence of diabetes. All the patients treated with the rigid dressing had primary healing of the amputation wound, while 13 patients treated with the conventional dressing had primary healing. Long term rehabilitation to the use of a prosthesis was not improved by the application of a rigid dressing on the amputation wound. Our current preference is use of the rigid dressing applied in the operating room at the time of amputation. If the wound is satisfactory and the general condition of the patient is such that he can use a temporary pylon, it is attached to the cast at the first change, about two weeks after operation.", "contents": "Evaluation of use of the rigid dressing in amputation of the lower extremity. Twenty-seven arteriosclerotic amputees were evaluated to determine the value of the rigid dressing technique for amputation wound management. Results were compared with those for patients having conventional stump wound management. The two groups were similar in number, age and incidence of diabetes. All the patients treated with the rigid dressing had primary healing of the amputation wound, while 13 patients treated with the conventional dressing had primary healing. Long term rehabilitation to the use of a prosthesis was not improved by the application of a rigid dressing on the amputation wound. Our current preference is use of the rigid dressing applied in the operating room at the time of amputation. If the wound is satisfactory and the general condition of the patient is such that he can use a temporary pylon, it is attached to the cast at the first change, about two weeks after operation.", "PMID": 959961}
{"id": "pubmed23n0032_3714", "title": "Reversal of renal failure and control of hypertension in patients with occlusion of the renal artery.", "content": "Results of arteriographic investigation of patients with deteriorating renal function or poorly controlled hypertension have revealed that thrombosis of the renal artery is not an uncommon exacerbating factor. Seventeen patients with one or more occluded renal arteries had an operation to improve renal function or to control hypertension. Stenosis of the contralateral renal artery was present in addition to the occlusion in four patients. Reconstructive arterial procedures were performed in 15 patients and nephrectomy was performed in two. Eight patients with renal failure had marked improvement in renal function after revascularization of the occluded renal arteries. The group had a mean preoperative serum creatinine value of 7.95+/-1.81 (S.E.) milligrams per cent which fell postoperatively to 3.91+/-1.21 (S.E.) milligrams per cent at a mean follow-up period 20 months. Preoperative control of hypertension was difficult in 16 of the 17 patients. Postoperatively, the blood pressure fell to normal levels in six patients, and in an additional eight patients, it did so with the administration of antihypertension therapy. The hypertension was unchanged in two patients. Plasma renin activity was measured in 14 of the patients with hypertension. It was elevated in 13 patients and normal in one patient. Postoperatively, the blood pressure was unchanged in the patient with normal plasma renin activity, but in 12 of the 13 patients with elevated plasma renin activity, the blood pressure returned to normal levels. It is concluded that patients with occluded renal arteries should be treated surgically. The major benefits of an aggressive approach to this condition are reversal of renal failure and control of hypertension.", "contents": "Reversal of renal failure and control of hypertension in patients with occlusion of the renal artery. Results of arteriographic investigation of patients with deteriorating renal function or poorly controlled hypertension have revealed that thrombosis of the renal artery is not an uncommon exacerbating factor. Seventeen patients with one or more occluded renal arteries had an operation to improve renal function or to control hypertension. Stenosis of the contralateral renal artery was present in addition to the occlusion in four patients. Reconstructive arterial procedures were performed in 15 patients and nephrectomy was performed in two. Eight patients with renal failure had marked improvement in renal function after revascularization of the occluded renal arteries. The group had a mean preoperative serum creatinine value of 7.95+/-1.81 (S.E.) milligrams per cent which fell postoperatively to 3.91+/-1.21 (S.E.) milligrams per cent at a mean follow-up period 20 months. Preoperative control of hypertension was difficult in 16 of the 17 patients. Postoperatively, the blood pressure fell to normal levels in six patients, and in an additional eight patients, it did so with the administration of antihypertension therapy. The hypertension was unchanged in two patients. Plasma renin activity was measured in 14 of the patients with hypertension. It was elevated in 13 patients and normal in one patient. Postoperatively, the blood pressure was unchanged in the patient with normal plasma renin activity, but in 12 of the 13 patients with elevated plasma renin activity, the blood pressure returned to normal levels. It is concluded that patients with occluded renal arteries should be treated surgically. The major benefits of an aggressive approach to this condition are reversal of renal failure and control of hypertension.", "PMID": 959962}
{"id": "pubmed23n0032_3715", "title": "Accuracy of Doppler ultrasound in clinically suspected venous thrombosis of the calf.", "content": "Doppler ultrasonic examinations and contrast phlebography were performed on 55 limbs of 43 patients with a clinical diagnosis of venous thrombosis of the calf and without phlebographic evidence of thrombosis above the knee. There was diagnostic concurrence of Doppler and venographic evaluations in 46 of 55 limbs studied. There was only one instance of a false-negative diagnosis by Doppler examination. Of the eight false-positive Doppler examination findings, an alternate clinical diagnosis affecting venous flow in the calf was evident in six patients. The diagnostic importance of augmentation of flow velocity of the posterior tibial vein upon release of compression of the calf is emphasized. A normal Doppler examination finding may accurately exclude significant deep venous disease in a patient with clinical manifestations of venous thrombosis in the calf.", "contents": "Accuracy of Doppler ultrasound in clinically suspected venous thrombosis of the calf. Doppler ultrasonic examinations and contrast phlebography were performed on 55 limbs of 43 patients with a clinical diagnosis of venous thrombosis of the calf and without phlebographic evidence of thrombosis above the knee. There was diagnostic concurrence of Doppler and venographic evaluations in 46 of 55 limbs studied. There was only one instance of a false-negative diagnosis by Doppler examination. Of the eight false-positive Doppler examination findings, an alternate clinical diagnosis affecting venous flow in the calf was evident in six patients. The diagnostic importance of augmentation of flow velocity of the posterior tibial vein upon release of compression of the calf is emphasized. A normal Doppler examination finding may accurately exclude significant deep venous disease in a patient with clinical manifestations of venous thrombosis in the calf.", "PMID": 959963}
{"id": "pubmed23n0032_3716", "title": "Beneficial effect of prostaglandin E1 in experimental hemorrhagic shock.", "content": "Twelve dogs were subjected to hemorrhagic shock by means of a modified Wiggers technique. Half were treated with prostaglandin E1, 1 microgram per kilogram per minute in 60 milliliters of saline solution, for one hour after shock, while the other half received only an equal amount of saline solution. Those dogs treated with prostaglandin E1 exhibited a significantly increased survival time and cardiac output as well as a significantly lowered total peripheral resistance when compared with those for untreated dogs. The various modes of action by which prostaglandin E1 exerts both its cardiovascular and other long term effects indicate a multisystem effect of the drug.", "contents": "Beneficial effect of prostaglandin E1 in experimental hemorrhagic shock. Twelve dogs were subjected to hemorrhagic shock by means of a modified Wiggers technique. Half were treated with prostaglandin E1, 1 microgram per kilogram per minute in 60 milliliters of saline solution, for one hour after shock, while the other half received only an equal amount of saline solution. Those dogs treated with prostaglandin E1 exhibited a significantly increased survival time and cardiac output as well as a significantly lowered total peripheral resistance when compared with those for untreated dogs. The various modes of action by which prostaglandin E1 exerts both its cardiovascular and other long term effects indicate a multisystem effect of the drug.", "PMID": 959964}
{"id": "pubmed23n0032_3717", "title": "The mechanism of capillarity in surgical dressings.", "content": "By appreciating the concept of capillarity and by determining whether or not the dressing to be used next to the wound should be hydrophilic or hydrophobic, a dressing can be designed that acts to remove secretions from a draining wound. It also may protect a wound from exogenous secretions.", "contents": "The mechanism of capillarity in surgical dressings. By appreciating the concept of capillarity and by determining whether or not the dressing to be used next to the wound should be hydrophilic or hydrophobic, a dressing can be designed that acts to remove secretions from a draining wound. It also may protect a wound from exogenous secretions.", "PMID": 959967}
{"id": "pubmed23n0032_3718", "title": "Anatomic localization and step by step advancement of the fiberoptic colonoscope.", "content": "Anatomic localization of the tip of a colonoscope without the aid of fluoroscopy is adequate in a majority of patients. External check points of transilluminated light within the colon on the abdominal wall, characteristic internal appearance of the colon and gross anatomic landmarks have been used. An improved technique for advancement results if a routine sequence of maneuvers is used in a similar manner in all patients.", "contents": "Anatomic localization and step by step advancement of the fiberoptic colonoscope. Anatomic localization of the tip of a colonoscope without the aid of fluoroscopy is adequate in a majority of patients. External check points of transilluminated light within the colon on the abdominal wall, characteristic internal appearance of the colon and gross anatomic landmarks have been used. An improved technique for advancement results if a routine sequence of maneuvers is used in a similar manner in all patients.", "PMID": 959968}
{"id": "pubmed23n0032_3719", "title": "The effects of coronary vasodilatation on cardiac performance during endotoxin shock.", "content": "Results of studies have suggested that endotoxin and lowered coronary arterial perfusion pressures are detrimental to cardiac performance and lead to failure. Prevention of cardiac failure in the isolated canine heart preparation confronted with endotoxin and decreased coronary perfusion pressure was possible by perfusing these hearts with sodium nitroprusside. Prevention of failure was manifested by a lowered left ventricular endiastolic pressure and was associated with increased coronary flow and decreased coronary resistance with increased oxygen delivery and decreased oxygen extraction. Possible explanations for improved performance by dilator perfusion include increased delivery of oxygen and nutrients to myocardial tissue as well as a reduction of ventricular wall tension by dilating the coronary vascular skeleton. Prevention of extravasation of interstitial fluid into myocardial tissue by reducing overperfusion of potentially damaged coronary vessels could serve to maintain myocardial integrity and ventricular compliance. The potential use of such therapy warrants further study, with emphasis on evaluating the hemodynamics of the intact animal.", "contents": "The effects of coronary vasodilatation on cardiac performance during endotoxin shock. Results of studies have suggested that endotoxin and lowered coronary arterial perfusion pressures are detrimental to cardiac performance and lead to failure. Prevention of cardiac failure in the isolated canine heart preparation confronted with endotoxin and decreased coronary perfusion pressure was possible by perfusing these hearts with sodium nitroprusside. Prevention of failure was manifested by a lowered left ventricular endiastolic pressure and was associated with increased coronary flow and decreased coronary resistance with increased oxygen delivery and decreased oxygen extraction. Possible explanations for improved performance by dilator perfusion include increased delivery of oxygen and nutrients to myocardial tissue as well as a reduction of ventricular wall tension by dilating the coronary vascular skeleton. Prevention of extravasation of interstitial fluid into myocardial tissue by reducing overperfusion of potentially damaged coronary vessels could serve to maintain myocardial integrity and ventricular compliance. The potential use of such therapy warrants further study, with emphasis on evaluating the hemodynamics of the intact animal.", "PMID": 959969}
{"id": "pubmed23n0032_3720", "title": "Kinin activation in the blood of patients with sepsis.", "content": "To determine whether or not kinin activation in the blood during severe infection with gram-negative bacteria may be related to hemodynamic abnormalities encountered, blood prekallikrein, kallikrein inhibitor and kinin values in 2l surgical patients with sepsis were compared with those in normotensive and hypotensive states. Because of reduced prekallikrein synthesis in patients with hepatic insufficiency, the normotensive and hypotensive groups were each subdivided according to the presence or absence of liver dysfunction, as indicated by elevated blood bilirubin, serum glutamic-oxalacetic transaminase or alkaline phosphatase levels. The mortality was zero in group 1, normotensive normal liver function; 80 per cent in group 2, hypotensive-normal liver function; 20 per cent in group 3, normotensive liver dysfunction, and 67 per cent in group 4, hypotensive liver dysfunction. Ultimately, the majority of deaths were due to respiratory failure. Although the blood prekallikrein level, was below normal in all groups and was significantly less in all patients with liver dysfunction, it was reduced proportionately in hypotensive patients to less than 30 per cent of the values noted in the two normotensive groups. This finding suggests prekallikrein consumption in the hypotensive groups to be the result of the process of activating kallikrein and bradykinin. This concept is supported by finding elevated kinin values, above 3 nanograms per milliliter of plasma, in only 28 per cent of those in group 1 and 12 per cent of those in group 3, while in the hypotensive patients, groups 2 and 4, the kinin level was elevated in 60 and 66 per cent, respectively.", "contents": "Kinin activation in the blood of patients with sepsis. To determine whether or not kinin activation in the blood during severe infection with gram-negative bacteria may be related to hemodynamic abnormalities encountered, blood prekallikrein, kallikrein inhibitor and kinin values in 2l surgical patients with sepsis were compared with those in normotensive and hypotensive states. Because of reduced prekallikrein synthesis in patients with hepatic insufficiency, the normotensive and hypotensive groups were each subdivided according to the presence or absence of liver dysfunction, as indicated by elevated blood bilirubin, serum glutamic-oxalacetic transaminase or alkaline phosphatase levels. The mortality was zero in group 1, normotensive normal liver function; 80 per cent in group 2, hypotensive-normal liver function; 20 per cent in group 3, normotensive liver dysfunction, and 67 per cent in group 4, hypotensive liver dysfunction. Ultimately, the majority of deaths were due to respiratory failure. Although the blood prekallikrein level, was below normal in all groups and was significantly less in all patients with liver dysfunction, it was reduced proportionately in hypotensive patients to less than 30 per cent of the values noted in the two normotensive groups. This finding suggests prekallikrein consumption in the hypotensive groups to be the result of the process of activating kallikrein and bradykinin. This concept is supported by finding elevated kinin values, above 3 nanograms per milliliter of plasma, in only 28 per cent of those in group 1 and 12 per cent of those in group 3, while in the hypotensive patients, groups 2 and 4, the kinin level was elevated in 60 and 66 per cent, respectively.", "PMID": 959970}
{"id": "pubmed23n0032_3721", "title": "Zinc and wound healing.", "content": "A review of the literature on zinc and wound healing shows the many gaps of knowledge which still exist. This study of the histologic appearance of wound healing in rats fed various diets demonstrates the serum zinc levels achieved by such diets. No significant improvement was observed in the rate of healing for any one group, but a number of histologic differences were observed.", "contents": "Zinc and wound healing. A review of the literature on zinc and wound healing shows the many gaps of knowledge which still exist. This study of the histologic appearance of wound healing in rats fed various diets demonstrates the serum zinc levels achieved by such diets. No significant improvement was observed in the rate of healing for any one group, but a number of histologic differences were observed.", "PMID": 959971}
{"id": "pubmed23n0032_3722", "title": "Duodenal anomalies and the ampulla of Vater.", "content": "The results of the surgical correction of congenital duodenal anomalies in infants free of severe prematurity, mongolism or other intestinal anomalies are most favorable. Several uncommon defects involving the second part of the duodenum, which required special attention to the anatomic proximity of the ampulla of Vater and the terminal portion of the common bile ducts have been encountered. The recognition of this intimate relationship may prevent inadvertent injuries to these structures, further improving the results of operative procedures for these difficult congenital malformations.", "contents": "Duodenal anomalies and the ampulla of Vater. The results of the surgical correction of congenital duodenal anomalies in infants free of severe prematurity, mongolism or other intestinal anomalies are most favorable. Several uncommon defects involving the second part of the duodenum, which required special attention to the anatomic proximity of the ampulla of Vater and the terminal portion of the common bile ducts have been encountered. The recognition of this intimate relationship may prevent inadvertent injuries to these structures, further improving the results of operative procedures for these difficult congenital malformations.", "PMID": 959972}
{"id": "pubmed23n0032_3723", "title": "Hydatid disease of the liver with thoracic involvement.", "content": "Eleven patients with hydatid disease of the liver with intrathoracic involvement have been treated at this hospital, and another patient was treated previously elsewhere. The clinical and roentgenologic symptoms depend greatly upon the evolutionary stage of the disease. General conditions are seriously affected in instances of ruptured and infected cysts. When the cysts open into the tracheobronchial tree as a result of coughing up pus in profuse quantities, the general condition may improve to a certain degree, but bronchopulmonary symptoms increase at that moment. An important spitting of bile must alert us to the existence of biliary hypertension. This condition must be treated first to avoid recurrence of the process. According to our experience, surgical treatment is based upon correct drainage of the cystic intrahepatic cavity under the diaphragm, separating the pleural cavity completely from the intrahepatic; excision of the transdiaphragmatic fistulous tract; conservative indications for pulmonary resection, and possible existence of biliary hypertension before and after operation.", "contents": "Hydatid disease of the liver with thoracic involvement. Eleven patients with hydatid disease of the liver with intrathoracic involvement have been treated at this hospital, and another patient was treated previously elsewhere. The clinical and roentgenologic symptoms depend greatly upon the evolutionary stage of the disease. General conditions are seriously affected in instances of ruptured and infected cysts. When the cysts open into the tracheobronchial tree as a result of coughing up pus in profuse quantities, the general condition may improve to a certain degree, but bronchopulmonary symptoms increase at that moment. An important spitting of bile must alert us to the existence of biliary hypertension. This condition must be treated first to avoid recurrence of the process. According to our experience, surgical treatment is based upon correct drainage of the cystic intrahepatic cavity under the diaphragm, separating the pleural cavity completely from the intrahepatic; excision of the transdiaphragmatic fistulous tract; conservative indications for pulmonary resection, and possible existence of biliary hypertension before and after operation.", "PMID": 959973}
{"id": "pubmed23n0032_3724", "title": "Lactate metabolism in the dog during shock from hemorrhage, cardiac tamponade or endotoxin.", "content": "The elevated arterial lactate concentration in shock was investigated by measuring lactate production and clearance rate using a constant infusion of 14C-labeled lactate. In addition, the pathways of lactate metabolism were characterized by determining the percentage of lactate under-going oxidation and the percentage of the total carbon dioxide production which was derived from lactate. These measurements were performed on 16 normal dogs and on 23 dogs in a state of shock. Shock was induced by hemorrhage in ten, by controlled cardiac tamponade in seven and by endotoxin injection in six. In all of the dogs in a state of shock, there was a statistically significant increase in both the arterial lactate concentration and lactate turnover, while the lactate clearance decreased significantly. The percentage of the arterial lactate which underwent oxidation remained normal. The percentage of the total carbon dioxide production which was derived from lactate increased significantly, p less than 0.05, from 4.7 per cent in the normal dogs to 22.7 per cent in the dogs in a state of shock. Since both oxygen uptake and carbon dioxide production remain unchanged in shock, these data are consistent with an increased metabolism of substrates which from pyruvate and lactate as intermediary metabolites, that is, carbohydrates and certain amino acids, with a concomitant decrease in the metabolism of substrates which do not form pyruvate, that is, free fatty acids. In both the normal and shocked dogs, the arterial lactate concentration rose as the lactate production rate increased. Therefore, the elevated arterial lactate in shock was due to an increase in the lactate production and not to a lack of oxygen.", "contents": "Lactate metabolism in the dog during shock from hemorrhage, cardiac tamponade or endotoxin. The elevated arterial lactate concentration in shock was investigated by measuring lactate production and clearance rate using a constant infusion of 14C-labeled lactate. In addition, the pathways of lactate metabolism were characterized by determining the percentage of lactate under-going oxidation and the percentage of the total carbon dioxide production which was derived from lactate. These measurements were performed on 16 normal dogs and on 23 dogs in a state of shock. Shock was induced by hemorrhage in ten, by controlled cardiac tamponade in seven and by endotoxin injection in six. In all of the dogs in a state of shock, there was a statistically significant increase in both the arterial lactate concentration and lactate turnover, while the lactate clearance decreased significantly. The percentage of the arterial lactate which underwent oxidation remained normal. The percentage of the total carbon dioxide production which was derived from lactate increased significantly, p less than 0.05, from 4.7 per cent in the normal dogs to 22.7 per cent in the dogs in a state of shock. Since both oxygen uptake and carbon dioxide production remain unchanged in shock, these data are consistent with an increased metabolism of substrates which from pyruvate and lactate as intermediary metabolites, that is, carbohydrates and certain amino acids, with a concomitant decrease in the metabolism of substrates which do not form pyruvate, that is, free fatty acids. In both the normal and shocked dogs, the arterial lactate concentration rose as the lactate production rate increased. Therefore, the elevated arterial lactate in shock was due to an increase in the lactate production and not to a lack of oxygen.", "PMID": 959974}
{"id": "pubmed23n0032_3725", "title": "Risk factors in local surgical procedures for diabetic gangrene.", "content": "One hundred and seventy-two diabetic patients undergoing local operations of the foot distal to the ankle joint were reviewed to explore the preoperative prognostic indicators of operative failure. Severity of infection, as measured by preoperative temperature and white blood count, correlated with that of failure. Subcutaneous gas, as seen roentgenographically, was associated with failure in five of six patients. No particular operation or type of wound and no general or specific medical or laboratory parameter correlated with failure. In the four and one-half years of the study, an improved operative success rate could only be correlated with improved preoperative management of infection, as reflected in a declining yearly preoperative white blood count and temperature. Delay of the operation until maximum medical control of the infection has occurred should improve the initial success rate in these patients.", "contents": "Risk factors in local surgical procedures for diabetic gangrene. One hundred and seventy-two diabetic patients undergoing local operations of the foot distal to the ankle joint were reviewed to explore the preoperative prognostic indicators of operative failure. Severity of infection, as measured by preoperative temperature and white blood count, correlated with that of failure. Subcutaneous gas, as seen roentgenographically, was associated with failure in five of six patients. No particular operation or type of wound and no general or specific medical or laboratory parameter correlated with failure. In the four and one-half years of the study, an improved operative success rate could only be correlated with improved preoperative management of infection, as reflected in a declining yearly preoperative white blood count and temperature. Delay of the operation until maximum medical control of the infection has occurred should improve the initial success rate in these patients.", "PMID": 959975}
{"id": "pubmed23n0032_3726", "title": "Colloid oncotic pressure and levels of albumin and total protein during major surgical procedures.", "content": "From the results of this study, it appears that whole blood alone or red blood cells reconstituted with saline solution do not adequately replace the loss of albumin and concomitant decrease in colloid oncotic pressure that occur during extensive intraabdominal and intrathoracic operations. Since colloid oncotic pressure is a major factor in the restitution of intravascular volume from stores of interstitial fluid and since it may also play a role in the development of postoperative pulmonary problems, it is important to maintain a relatively normal colloid oncotic pressure during the operation and in the immediate postoperative period. Although dextran can be used for this purpose, its short half-life of four to six hours and associated coagulation problems militate against its use in large quantities. This leaves purified protein fraction or salt-poor albumin as the main sources of protein for the maintenance of colloid oncotic pressure. Both of these products are expensive and short in supply. The oncometer in present use is a clinically feasible and rapid, one to three minutes, means of determining the colloid oncotic pressure. It permits a rational approach to the use of albumin products, avoiding the pitfalls of under or excess administration in the operative and postoperative periods.", "contents": "Colloid oncotic pressure and levels of albumin and total protein during major surgical procedures. From the results of this study, it appears that whole blood alone or red blood cells reconstituted with saline solution do not adequately replace the loss of albumin and concomitant decrease in colloid oncotic pressure that occur during extensive intraabdominal and intrathoracic operations. Since colloid oncotic pressure is a major factor in the restitution of intravascular volume from stores of interstitial fluid and since it may also play a role in the development of postoperative pulmonary problems, it is important to maintain a relatively normal colloid oncotic pressure during the operation and in the immediate postoperative period. Although dextran can be used for this purpose, its short half-life of four to six hours and associated coagulation problems militate against its use in large quantities. This leaves purified protein fraction or salt-poor albumin as the main sources of protein for the maintenance of colloid oncotic pressure. Both of these products are expensive and short in supply. The oncometer in present use is a clinically feasible and rapid, one to three minutes, means of determining the colloid oncotic pressure. It permits a rational approach to the use of albumin products, avoiding the pitfalls of under or excess administration in the operative and postoperative periods.", "PMID": 959976}
{"id": "pubmed23n0032_3727", "title": "Rapid roentgenologic diagnosis of acute cholecystitis.", "content": "A rapid and safe method of roentgenographic evaluation of the acutely inflamed wall of the gallbladder, infusion tomography of the gallbladder, was performed 146 times, and the correlation with 67 subsequent cholecystectomies was reviewed. If the results of infusion tomogram of the gallbladder are abnormal, the gallbladder is diseased; if acute cholecystitis is present, the results of the examination will be abnormal in 96 per cent; if results of the infusion tomogram of the gallbladder are normal, the results are questionable, and oral cholecystography should be performed to evaluate  the condition further. If the results of a repeat dosage oral cholecystogram are abnormal, the gallbladder probably is diseased, even if the results of an infusion tomographic examination of the gallbladder are normal.", "contents": "Rapid roentgenologic diagnosis of acute cholecystitis. A rapid and safe method of roentgenographic evaluation of the acutely inflamed wall of the gallbladder, infusion tomography of the gallbladder, was performed 146 times, and the correlation with 67 subsequent cholecystectomies was reviewed. If the results of infusion tomogram of the gallbladder are abnormal, the gallbladder is diseased; if acute cholecystitis is present, the results of the examination will be abnormal in 96 per cent; if results of the infusion tomogram of the gallbladder are normal, the results are questionable, and oral cholecystography should be performed to evaluate  the condition further. If the results of a repeat dosage oral cholecystogram are abnormal, the gallbladder probably is diseased, even if the results of an infusion tomographic examination of the gallbladder are normal.", "PMID": 959977}
{"id": "pubmed23n0032_3728", "title": "Trauma of the bladder.", "content": "The records of 98 patients with trauma of the bladder were reviewed. Eighty per cent were injured as a result of blunt trauma, and 72 per cent of these patients had an associated fracture of the pelvis. The most common type of injury was a contusion, followed by an extraperitoneal rupture of the bladder. In all patients, diagnosis was established by the means of a retrograde cystogram. While all patients with intraperitoneal ruptures of the bladder were treated by surgical repair, half of those with extraperitoneal ruptures were successfully managed by catheter drainage alone. Complications of therapy consisted of bacterial cystitis, presumably as a result of catheter drainage.", "contents": "Trauma of the bladder. The records of 98 patients with trauma of the bladder were reviewed. Eighty per cent were injured as a result of blunt trauma, and 72 per cent of these patients had an associated fracture of the pelvis. The most common type of injury was a contusion, followed by an extraperitoneal rupture of the bladder. In all patients, diagnosis was established by the means of a retrograde cystogram. While all patients with intraperitoneal ruptures of the bladder were treated by surgical repair, half of those with extraperitoneal ruptures were successfully managed by catheter drainage alone. Complications of therapy consisted of bacterial cystitis, presumably as a result of catheter drainage.", "PMID": 959978}
{"id": "pubmed23n0032_3729", "title": "A simple plethysmographic method for differentiating primary from secondary varicose veins.", "content": "Characteristics of venous flow were studied in 81 normal legs and in 98 legs with varicose vein disorders. The change in the volume in the calf after calf muscle contractions was studied using the strain gauge technique with the patients in the erect position. During contractions of the calf muscle, the volume in the calf decreases, chiefly because of reduction of blood volume in the veins. After cessation of the contractions, there is a gradual return of volume to precontraction values. This was measured in seconds and called venous return time and in milliliters times 100 milliliters of tissue-1 times minutes-1 and called venous reflux flow. In normal persons, the venous return time was 21.9+/-6.7 (S.D.) seconds, and in patients with venous disorders, the corresponding time was 6.9+/-2.9 (S.D.) seconds. Venous reflux flow which was inversely correlated with the venous return time was 11.4+/-4.9 and 22.3+/-7.3 (S.D.), respectively. A good correlation was found between venous return time and venous pressure measurements. No age or sex differences were seen. In patients with superficial venous insufficiency, the venous return time and venous reflux time became normal when the diseased portion of the vein was compressed. In patients with insufficient perforating veins or damaged deep vein valves, external compression did not change venous flow characteristics. This indicates that the method can be used preoperatively to differentiate between primary and secondary varicose veins and, thus, influence the type of operation to be performed. The method can easily be applied for clinical purposes.", "contents": "A simple plethysmographic method for differentiating primary from secondary varicose veins. Characteristics of venous flow were studied in 81 normal legs and in 98 legs with varicose vein disorders. The change in the volume in the calf after calf muscle contractions was studied using the strain gauge technique with the patients in the erect position. During contractions of the calf muscle, the volume in the calf decreases, chiefly because of reduction of blood volume in the veins. After cessation of the contractions, there is a gradual return of volume to precontraction values. This was measured in seconds and called venous return time and in milliliters times 100 milliliters of tissue-1 times minutes-1 and called venous reflux flow. In normal persons, the venous return time was 21.9+/-6.7 (S.D.) seconds, and in patients with venous disorders, the corresponding time was 6.9+/-2.9 (S.D.) seconds. Venous reflux flow which was inversely correlated with the venous return time was 11.4+/-4.9 and 22.3+/-7.3 (S.D.), respectively. A good correlation was found between venous return time and venous pressure measurements. No age or sex differences were seen. In patients with superficial venous insufficiency, the venous return time and venous reflux time became normal when the diseased portion of the vein was compressed. In patients with insufficient perforating veins or damaged deep vein valves, external compression did not change venous flow characteristics. This indicates that the method can be used preoperatively to differentiate between primary and secondary varicose veins and, thus, influence the type of operation to be performed. The method can easily be applied for clinical purposes.", "PMID": 959979}
{"id": "pubmed23n0032_3730", "title": "The effect of hepatic bile on retained common duct stones.", "content": "Study of the time interval since cholecystectomy in 91 patients with symptomatic choledocholithiasis who presented after cholecystectomy led to the conclusion that retained common duct stones increase in size with the passage of time. The inference is that, in these patients bile remains supersaturated with cholesterol after cholecystectomy.", "contents": "The effect of hepatic bile on retained common duct stones. Study of the time interval since cholecystectomy in 91 patients with symptomatic choledocholithiasis who presented after cholecystectomy led to the conclusion that retained common duct stones increase in size with the passage of time. The inference is that, in these patients bile remains supersaturated with cholesterol after cholecystectomy.", "PMID": 959980}
{"id": "pubmed23n0032_3731", "title": "Microscopic technique for reversal of vasectomy.", "content": "This microscopic technique for reversal of a vasectomy requires intensive training on small animals in the laboratory before it can be attempted on humans. It has assured sperm in the ejaculate of all patients who have sperm in the proximal portion of the vas at the time of vasovasostomy, good sperm count and motility three to five months later in 90 per cent of them and early pregnancy of the spouse in 50 per cent. Longer follow-up periods will be necessary to estimate how high the eventual success rate will be, but it appears at this juncture that this approach is preferable to nonmicroscopic splinting techniques.", "contents": "Microscopic technique for reversal of vasectomy. This microscopic technique for reversal of a vasectomy requires intensive training on small animals in the laboratory before it can be attempted on humans. It has assured sperm in the ejaculate of all patients who have sperm in the proximal portion of the vas at the time of vasovasostomy, good sperm count and motility three to five months later in 90 per cent of them and early pregnancy of the spouse in 50 per cent. Longer follow-up periods will be necessary to estimate how high the eventual success rate will be, but it appears at this juncture that this approach is preferable to nonmicroscopic splinting techniques.", "PMID": 959982}
{"id": "pubmed23n0032_3732", "title": "Continuous intracranial pressure monitoring: review of techniques and presentation of method.", "content": "Continuous monitoring of intracranial pressure has proved to be of value in guiding the treatment of patients with diffuse cerebral trauma or edema. The evolution of monitoring devices is reviewed from simple lumbar puncture through intraventricular, subdural, epidural and external monitoring with progressively more sophisticated equipment. The device reported in detail connects the subarachnoid space to an on-line data reducer that calculates the percentage time the intracranial pressure is in 16 pressure ranges of 5 mmHg each and portrays it on a histogram. The pressure at that moment, the total range of the intracranial pressure, the level of pressure the patient has had for the largest percented of time, and pressure waves, if present, can all be determined by this process.", "contents": "Continuous intracranial pressure monitoring: review of techniques and presentation of method. Continuous monitoring of intracranial pressure has proved to be of value in guiding the treatment of patients with diffuse cerebral trauma or edema. The evolution of monitoring devices is reviewed from simple lumbar puncture through intraventricular, subdural, epidural and external monitoring with progressively more sophisticated equipment. The device reported in detail connects the subarachnoid space to an on-line data reducer that calculates the percentage time the intracranial pressure is in 16 pressure ranges of 5 mmHg each and portrays it on a histogram. The pressure at that moment, the total range of the intracranial pressure, the level of pressure the patient has had for the largest percented of time, and pressure waves, if present, can all be determined by this process.", "PMID": 959984}
{"id": "pubmed23n0032_3733", "title": "The femoral nerve traction test with lumbar disc protrusions.", "content": "A easily performed test which can be carried out at the patient's bedside has been described. It is referred to as the femoral nerve traction test. Clinical evidence suggests it may have merit as an aid in the diagnosis of disc herniations that affect the upper lumbar spine.", "contents": "The femoral nerve traction test with lumbar disc protrusions. A easily performed test which can be carried out at the patient's bedside has been described. It is referred to as the femoral nerve traction test. Clinical evidence suggests it may have merit as an aid in the diagnosis of disc herniations that affect the upper lumbar spine.", "PMID": 959987}
{"id": "pubmed23n0032_3734", "title": "Angiographic demonstration of vertebral osteoid osteoma.", "content": "A case of osteoid osteoma in the left lamina of the T-8 vertebra is reported. The roentgenograms of the thoracic spine showed a hyperostotic round mass with associated scoliotic changes and the myelograms revealed complete obstruction of the spinal subarachnoid space. By selective spinal angiography a peculiar ring-like vascular stain was visualized in the area of hyperostosis which may be characteristic of an osteoid osteoma.", "contents": "Angiographic demonstration of vertebral osteoid osteoma. A case of osteoid osteoma in the left lamina of the T-8 vertebra is reported. The roentgenograms of the thoracic spine showed a hyperostotic round mass with associated scoliotic changes and the myelograms revealed complete obstruction of the spinal subarachnoid space. By selective spinal angiography a peculiar ring-like vascular stain was visualized in the area of hyperostosis which may be characteristic of an osteoid osteoma.", "PMID": 959988}
{"id": "pubmed23n0032_3735", "title": "Prolonged, profound hypotension produced safely with sodium nitroprusside.", "content": "Nine and one-fourth hours of profound hypotension were necessary to accomplish the successful removal of a large arteriovenous malformation from the medial portion of the dominant parietal lobe. The hypotensive agent used was sodium nitroprusside. The removal was associated with a minimal neurological deficit. This is the longest known use of sodium nitroprusside for deliberate hypotension during surgery which emphasizes the safety of this hypotensive technique, provided the total dosage of sodium nitroprusside is reasonable.", "contents": "Prolonged, profound hypotension produced safely with sodium nitroprusside. Nine and one-fourth hours of profound hypotension were necessary to accomplish the successful removal of a large arteriovenous malformation from the medial portion of the dominant parietal lobe. The hypotensive agent used was sodium nitroprusside. The removal was associated with a minimal neurological deficit. This is the longest known use of sodium nitroprusside for deliberate hypotension during surgery which emphasizes the safety of this hypotensive technique, provided the total dosage of sodium nitroprusside is reasonable.", "PMID": 959989}
{"id": "pubmed23n0032_3736", "title": "Preferential cerebral hypothermia with elective cardiac arrest: resection of \"giant\" aneurysm.", "content": "The technique of preferential cerebral hypothermia is reported in its application to a patient with a \"giant\" anterior communicating artery aneurysm. The method utilizes elective ventricular fibrillation and differential or \"preferential\" hypothermia induced by a combination of external skin cooling and perfusion of core organs with 0 degree buffered electrolyte solution. The value of the technique lies in its provision of a period of safe circulatory arrest approaching one hour without the need for anticoagulation, heart-lung bypass, open chest resuscitation or major vessel clamping. Because of the absence of blood flow and because of the clear fluid washout of the cerebral vessels, it was possible to open the aneurysm, evacuate its contents and resect it in several sections. It was not necessary to clip the feeding arteries until all dissection and total removal of the aneurysm were completed. The application of the technique to neurosurgery and cardiovascular surgery is discussed.", "contents": "Preferential cerebral hypothermia with elective cardiac arrest: resection of \"giant\" aneurysm. The technique of preferential cerebral hypothermia is reported in its application to a patient with a \"giant\" anterior communicating artery aneurysm. The method utilizes elective ventricular fibrillation and differential or \"preferential\" hypothermia induced by a combination of external skin cooling and perfusion of core organs with 0 degree buffered electrolyte solution. The value of the technique lies in its provision of a period of safe circulatory arrest approaching one hour without the need for anticoagulation, heart-lung bypass, open chest resuscitation or major vessel clamping. Because of the absence of blood flow and because of the clear fluid washout of the cerebral vessels, it was possible to open the aneurysm, evacuate its contents and resect it in several sections. It was not necessary to clip the feeding arteries until all dissection and total removal of the aneurysm were completed. The application of the technique to neurosurgery and cardiovascular surgery is discussed.", "PMID": 959990}
{"id": "pubmed23n0032_3737", "title": "Neuroepithelial Cyst of the cerebellar vermis.", "content": "A cyst located in the cerebellar vermis and causing obstructive hydrocephalus is described. The wall of the cyst was lined with ciliated and non-ciliated cuboidal epithelial cells which were completely invested by a basement membrane.", "contents": "Neuroepithelial Cyst of the cerebellar vermis. A cyst located in the cerebellar vermis and causing obstructive hydrocephalus is described. The wall of the cyst was lined with ciliated and non-ciliated cuboidal epithelial cells which were completely invested by a basement membrane.", "PMID": 959991}
{"id": "pubmed23n0032_3738", "title": "Diagnosis of intrathalamic cyst by computerized tomographic scan.", "content": "A case of an intrathalamic cyst initially misdiagnosed as a thalamic glioma is reported. It was followed for five years before a computerized tomographic scan led to a correct diagnosis. A simple means of stereotaxically draining the lesion is illustrated.", "contents": "Diagnosis of intrathalamic cyst by computerized tomographic scan. A case of an intrathalamic cyst initially misdiagnosed as a thalamic glioma is reported. It was followed for five years before a computerized tomographic scan led to a correct diagnosis. A simple means of stereotaxically draining the lesion is illustrated.", "PMID": 959993}
{"id": "pubmed23n0032_3739", "title": "Arteriovenous malformations of vein of Galen: microsurgical treatment.", "content": "The surgical technic used at the neurosurgical clinic in Z\u00fcrich in eradicating arteriovenous malformations of the vein of Galen is described. It is based on experience with nine cases. The surgical approach is made posteriorly. The right occipital lobe is retracted laterally from the falx cerebri. The multiple feeding vessels demonstrated in the angiogram are occluded; first, on the right side, then the left, and finally beneath the malformation. The draining vein of Galen is not occluded or removed. The use of the operating microscope is essential.", "contents": "Arteriovenous malformations of vein of Galen: microsurgical treatment. The surgical technic used at the neurosurgical clinic in Z\u00fcrich in eradicating arteriovenous malformations of the vein of Galen is described. It is based on experience with nine cases. The surgical approach is made posteriorly. The right occipital lobe is retracted laterally from the falx cerebri. The multiple feeding vessels demonstrated in the angiogram are occluded; first, on the right side, then the left, and finally beneath the malformation. The draining vein of Galen is not occluded or removed. The use of the operating microscope is essential.", "PMID": 959995}
{"id": "pubmed23n0032_3740", "title": "Benign and malignant teratomas in children: analysis of 85 patients.", "content": "Teratomas were observed in 85 infants and children. Fifty-eight (68%) were girls and 27 (32%) boys. Site of origin was sacrococcygeal in 55 patients (64.8%), mediastinal in ten (11.7%), gonadal in ten (11.7%), presacral in four (4.8%), retroperitoneal in three (3.5%), and cervical in three (3.5%). Sixty-seven (78.8%) teratomas were benign and 18 (21.1%) malignant. Malignant tumors were noted in 11 of 55 sacrococcygeal (20%), two of ten mediastinal (20%), three of eight ovarian (37.5%), and both testicular lesions. Cervical, retroperitoneal, and presacral tumors were benign. Age at diagnosis (greater than 1 month), presence of symptoms (urinary and colonic obstruction), and serum positive for alpha fetoprotein were indicators of malignancy in sacrococcygeal cases. Age was not a factor for teratomas at other sites. Tumor size, presence of calcification, and gross appearance (cystic or solid) did not relate to the tumor's benign or malignant nature. Three of four presacral tumors were associated with anorectal anomalies. Operative resection is the treatment of choice. Four deaths were related to operative hemorrhage. Four of five survivors with malignant teratoma received radiation and chemotherapy. A more aggressive role for combined adjunctive measures is suggested in cases of malignancy.", "contents": "Benign and malignant teratomas in children: analysis of 85 patients. Teratomas were observed in 85 infants and children. Fifty-eight (68%) were girls and 27 (32%) boys. Site of origin was sacrococcygeal in 55 patients (64.8%), mediastinal in ten (11.7%), gonadal in ten (11.7%), presacral in four (4.8%), retroperitoneal in three (3.5%), and cervical in three (3.5%). Sixty-seven (78.8%) teratomas were benign and 18 (21.1%) malignant. Malignant tumors were noted in 11 of 55 sacrococcygeal (20%), two of ten mediastinal (20%), three of eight ovarian (37.5%), and both testicular lesions. Cervical, retroperitoneal, and presacral tumors were benign. Age at diagnosis (greater than 1 month), presence of symptoms (urinary and colonic obstruction), and serum positive for alpha fetoprotein were indicators of malignancy in sacrococcygeal cases. Age was not a factor for teratomas at other sites. Tumor size, presence of calcification, and gross appearance (cystic or solid) did not relate to the tumor's benign or malignant nature. Three of four presacral tumors were associated with anorectal anomalies. Operative resection is the treatment of choice. Four deaths were related to operative hemorrhage. Four of five survivors with malignant teratoma received radiation and chemotherapy. A more aggressive role for combined adjunctive measures is suggested in cases of malignancy.", "PMID": 960000}
{"id": "pubmed23n0032_3741", "title": "Unequal pupils. A flow chart for sorting out the anisocorias.", "content": "When anisocoria is present, the ophthalmologist knows that something is wrong. However, location of the lesion and diagnosis of the problem can be difficult. A step-by-step flow chart for diagnosis of unequal pupils is presented, with explantation of various signs and descriptions of appropriate diagnostic tests.", "contents": "Unequal pupils. A flow chart for sorting out the anisocorias. When anisocoria is present, the ophthalmologist knows that something is wrong. However, location of the lesion and diagnosis of the problem can be difficult. A step-by-step flow chart for diagnosis of unequal pupils is presented, with explantation of various signs and descriptions of appropriate diagnostic tests.", "PMID": 959997}
{"id": "pubmed23n0032_3742", "title": "Noninvasive ultrasonic carotid angiography: prospective validation by contrast arteriography.", "content": "Pulsed ultrasonic images of the carotid bifurcation in 82 vessels of 43 patients were compared independently with contrast arteriograms for stenosis (by percentage quartiles) or occlusion of the internal carotid artery. All 14 occluded vessels were identified correctly by ultrasound but were visualized on repeat examination. Estimation of percentage stenosis on ultrasonic images agreed with the quartile determination by contrast arteriography in 35 of 68 (51 percent) vessels and was within one quartile of correct interpretation in 48 of 68 (71 percent). The interpretative error of grading stenosis of ultrasonic images was due to vascular wall calcification which inhibited ultrasound transmission. This limitation was overcome by sound spectral (sonographic) analysis of distal internal carotid flow velocity which allowed estimation of stenosis within one quartile of that determined by contrast arteriography in 46 of 47 (98 percent) vessels.", "contents": "Noninvasive ultrasonic carotid angiography: prospective validation by contrast arteriography. Pulsed ultrasonic images of the carotid bifurcation in 82 vessels of 43 patients were compared independently with contrast arteriograms for stenosis (by percentage quartiles) or occlusion of the internal carotid artery. All 14 occluded vessels were identified correctly by ultrasound but were visualized on repeat examination. Estimation of percentage stenosis on ultrasonic images agreed with the quartile determination by contrast arteriography in 35 of 68 (51 percent) vessels and was within one quartile of correct interpretation in 48 of 68 (71 percent). The interpretative error of grading stenosis of ultrasonic images was due to vascular wall calcification which inhibited ultrasound transmission. This limitation was overcome by sound spectral (sonographic) analysis of distal internal carotid flow velocity which allowed estimation of stenosis within one quartile of that determined by contrast arteriography in 46 of 47 (98 percent) vessels.", "PMID": 960001}
{"id": "pubmed23n0032_3743", "title": "Middle cerebral artery microneurosurgical embolectomy.", "content": "Cerebral embolism carries significant mortality and morbidity rates. Middle cerebral artery embolectomies which have been carried out in man with variable results, have been reported by different authors, In order to define a time limit for embolectomies before irreversible damage has been incurred, an experimental embolus model was used in dogs. It was observed that embolectomy carried out at 2, 3, and up to 5 hours after embolism was relatively safe and effective. Beyond 5 hours, embolectomy carried an increased risk of death and morbidity.", "contents": "Middle cerebral artery microneurosurgical embolectomy. Cerebral embolism carries significant mortality and morbidity rates. Middle cerebral artery embolectomies which have been carried out in man with variable results, have been reported by different authors, In order to define a time limit for embolectomies before irreversible damage has been incurred, an experimental embolus model was used in dogs. It was observed that embolectomy carried out at 2, 3, and up to 5 hours after embolism was relatively safe and effective. Beyond 5 hours, embolectomy carried an increased risk of death and morbidity.", "PMID": 960002}
{"id": "pubmed23n0032_3744", "title": "The exfoliation syndrome: source of the fibrillar material on the capsule.", "content": "A clinical case of unilateral capsular exfoliation syndrome was observed in the previously traumatized eye of a 22-year-old man with a drawn-down pupil. The distribution of material correspond to the folds of the inner surface of contiguous iris. The periphery of the lens appeared free of deposits. These findings contradict previous theories on the source of the fibrillar material from the lens epithelium and indicate that it is derived from the iris pigment epithelium and not from within the lens capsule, although there is no question but that the lens is involved in a similar process, the products of which probably do not reach the lens surface. The unilateral occurrence of the exfoliation syndrome in a traumatized eye of a 22-year-old man is unique and cannot be explained at this time.", "contents": "The exfoliation syndrome: source of the fibrillar material on the capsule. A clinical case of unilateral capsular exfoliation syndrome was observed in the previously traumatized eye of a 22-year-old man with a drawn-down pupil. The distribution of material correspond to the folds of the inner surface of contiguous iris. The periphery of the lens appeared free of deposits. These findings contradict previous theories on the source of the fibrillar material from the lens epithelium and indicate that it is derived from the iris pigment epithelium and not from within the lens capsule, although there is no question but that the lens is involved in a similar process, the products of which probably do not reach the lens surface. The unilateral occurrence of the exfoliation syndrome in a traumatized eye of a 22-year-old man is unique and cannot be explained at this time.", "PMID": 959998}
{"id": "pubmed23n0032_3745", "title": "Carotid body tumors.", "content": "Carotid body tumors are encounterd infrequently. During the past 30 years, 15 patients with 16 carotid body tumors have been treated at our medical center. All patients have been followed for a minimum of 7 years. The major findings relate to the areas of greatest controversy concerning carotid body tumor treatment: the need for excision, the value of preoperative arteriography, the role of radiation therapy, the criteria of malignancy, and the indications for and technique of carotid artery replacement. Discussed at length are our conclusions that (1) arteriography is absolutely diagnostic and therefore mandatory when carotid body tumor is suspected; (2) radiation therapy has little if any value and has increased the difficulty of excision; and (3) all carotid body tumors should be considered to be malignant. The familial tendency of this tumor is explored in relation to this last conclusion, and we report what we believe is the first finding of both a \"benign\" and \"malignant\" tumor occurring in the same family. The techniques of subadventitial excision and carotid artery replacement with autogenous saphenous vein, the latter necessary on three occasions, are demonstrated. There were no operative deaths, no serious complications after operation, and no recurrence or metastasis following total excision. However, two patients died as a direct result of tumor growth following biopsy without excision.", "contents": "Carotid body tumors. Carotid body tumors are encounterd infrequently. During the past 30 years, 15 patients with 16 carotid body tumors have been treated at our medical center. All patients have been followed for a minimum of 7 years. The major findings relate to the areas of greatest controversy concerning carotid body tumor treatment: the need for excision, the value of preoperative arteriography, the role of radiation therapy, the criteria of malignancy, and the indications for and technique of carotid artery replacement. Discussed at length are our conclusions that (1) arteriography is absolutely diagnostic and therefore mandatory when carotid body tumor is suspected; (2) radiation therapy has little if any value and has increased the difficulty of excision; and (3) all carotid body tumors should be considered to be malignant. The familial tendency of this tumor is explored in relation to this last conclusion, and we report what we believe is the first finding of both a \"benign\" and \"malignant\" tumor occurring in the same family. The techniques of subadventitial excision and carotid artery replacement with autogenous saphenous vein, the latter necessary on three occasions, are demonstrated. There were no operative deaths, no serious complications after operation, and no recurrence or metastasis following total excision. However, two patients died as a direct result of tumor growth following biopsy without excision.", "PMID": 960003}
{"id": "pubmed23n0032_3746", "title": "Application of contemporary reconstructive techniques in head and neck surgery for anterior oral-facial cancers.", "content": "One hundred and seventy-eight patients underwent surgical therapy for oral and cervical cancers from 1964 to 1975. About 25 percent of the patients underwent neck dissection and/or \"pull-through\" procedures. However, majority of patients required a spectrum of reconstructive techniques extending from marginal mandibulectomy with or without skin flaps (39), partial mandibulectomy with immediate prosthetic mandible reconstruction (36), to extended resections with skin flaps or staged reconstructive procedures (48). The advantages and disadvantages of each reconstructive procedure have been observed and a scheme of graded management has been developed. The therapeutic goal is to maximize functional oral reconstruction without compromising tumor cure. There were two operative deaths--one from myocardial infarction after operation and one from halothane hepatitis. The tumors were grouped according to TNM classification. In the follow-up of the 178 patients, 47 per cent are known to be alive and free of tumor. The better results (greater than 70% free of tumor) are in the group with smaller tumors (less than 2 cm.) and no node involvement, and there are less favorable rates for those patients with larger tumors and nodal metastasis or invasion of adjacent structures. There was a 49 percent 2 year survival rate and 12 of the deaths were from nontumor causes. Ninety percent of these patients smoked more than one pack of cigarettes per day, accounting for the high rate of synchronous or subsequent oroairway cancers (7 percent). Seventy-five percent were considered to be \"heavy alcoholics\" with evidence of cirrhotic liver disease. These two factors significantly decreased the survival from rate 54 to 47 percent. The series shows that planned primary reconstructive surgery can be done at a low risk, that it can enhance resectability of head and neck cancers, and that it does improve oral function after operation.", "contents": "Application of contemporary reconstructive techniques in head and neck surgery for anterior oral-facial cancers. One hundred and seventy-eight patients underwent surgical therapy for oral and cervical cancers from 1964 to 1975. About 25 percent of the patients underwent neck dissection and/or \"pull-through\" procedures. However, majority of patients required a spectrum of reconstructive techniques extending from marginal mandibulectomy with or without skin flaps (39), partial mandibulectomy with immediate prosthetic mandible reconstruction (36), to extended resections with skin flaps or staged reconstructive procedures (48). The advantages and disadvantages of each reconstructive procedure have been observed and a scheme of graded management has been developed. The therapeutic goal is to maximize functional oral reconstruction without compromising tumor cure. There were two operative deaths--one from myocardial infarction after operation and one from halothane hepatitis. The tumors were grouped according to TNM classification. In the follow-up of the 178 patients, 47 per cent are known to be alive and free of tumor. The better results (greater than 70% free of tumor) are in the group with smaller tumors (less than 2 cm.) and no node involvement, and there are less favorable rates for those patients with larger tumors and nodal metastasis or invasion of adjacent structures. There was a 49 percent 2 year survival rate and 12 of the deaths were from nontumor causes. Ninety percent of these patients smoked more than one pack of cigarettes per day, accounting for the high rate of synchronous or subsequent oroairway cancers (7 percent). Seventy-five percent were considered to be \"heavy alcoholics\" with evidence of cirrhotic liver disease. These two factors significantly decreased the survival from rate 54 to 47 percent. The series shows that planned primary reconstructive surgery can be done at a low risk, that it can enhance resectability of head and neck cancers, and that it does improve oral function after operation.", "PMID": 960004}
{"id": "pubmed23n0032_3747", "title": "Hypercalcemia in patients with known malignent disease.", "content": "In 82 patients, a preoperative diagnosis of primary hyperparathyroidism has been established by means of transfemoral neck vein catheterization and measurement of serum immunoreactive parathyroid hormone (iPTH). Twenty-five of these patients have had cancer in other parts of the body but with no evidence of recurrence or metastasis. One patient had carcinoma of the colon with metastases, and four were members of families with multiple endocrine adenomatosis (MEA, Types I and II). In six other hypercalcemic patients, high levels of iPTH were found also in the effluent blood from cancer sites other than the parathyroid gland, secondary to ectopic hormone production or pseudohyperparathyroidism. In addition, a high serum level of iPTH was found in the superior vena cava of a seventh patient who had carcinoma of the breast but no clinical or radiological signs of recurrence or metastasis with the exception of an enlarged liver. This iPTH finding was interpreted as being, probably, the result of parathyroid adenoma in either the neck or the mediastinum. At the time of operation, a transcervical mediastinal search was made. Four normal cervical parathyroid glands were found; three were removed. Hypercalcemia persisted after operation, and the patient died. At postmortem examination, microscopic study revealed that the disease had metastasized to lungs and hilar lymph nodes. There was massive metastasis in the liver; the liver contained a large amount of iPTH. The results of these investigations suggest that (1) venous catheterization of the neck veins and the effluent blood from extraparathyroid tumors aid in identifying and localizing iPTH production; (2) primary benign hyperparathyroidism is not uncommon in patients with cancer, and its co-existence must be recognized; (3) high serum iPTH level in the superior vena cava may be found in patients with metastatic or primary cancer of the thoracic cavity; and (4) hyperparathyroidism may be the first hint of a familial multiple endocrine syndrome.", "contents": "Hypercalcemia in patients with known malignent disease. In 82 patients, a preoperative diagnosis of primary hyperparathyroidism has been established by means of transfemoral neck vein catheterization and measurement of serum immunoreactive parathyroid hormone (iPTH). Twenty-five of these patients have had cancer in other parts of the body but with no evidence of recurrence or metastasis. One patient had carcinoma of the colon with metastases, and four were members of families with multiple endocrine adenomatosis (MEA, Types I and II). In six other hypercalcemic patients, high levels of iPTH were found also in the effluent blood from cancer sites other than the parathyroid gland, secondary to ectopic hormone production or pseudohyperparathyroidism. In addition, a high serum level of iPTH was found in the superior vena cava of a seventh patient who had carcinoma of the breast but no clinical or radiological signs of recurrence or metastasis with the exception of an enlarged liver. This iPTH finding was interpreted as being, probably, the result of parathyroid adenoma in either the neck or the mediastinum. At the time of operation, a transcervical mediastinal search was made. Four normal cervical parathyroid glands were found; three were removed. Hypercalcemia persisted after operation, and the patient died. At postmortem examination, microscopic study revealed that the disease had metastasized to lungs and hilar lymph nodes. There was massive metastasis in the liver; the liver contained a large amount of iPTH. The results of these investigations suggest that (1) venous catheterization of the neck veins and the effluent blood from extraparathyroid tumors aid in identifying and localizing iPTH production; (2) primary benign hyperparathyroidism is not uncommon in patients with cancer, and its co-existence must be recognized; (3) high serum iPTH level in the superior vena cava may be found in patients with metastatic or primary cancer of the thoracic cavity; and (4) hyperparathyroidism may be the first hint of a familial multiple endocrine syndrome.", "PMID": 960005}
{"id": "pubmed23n0032_3748", "title": "Myopia--a treatable \"disease\"?", "content": "The authors cite various information and misinformation regarding proposed treatments (atropinization, contact lenses, orthokeratology, wearing glasses, not wearing glasses) for the progression of myopia. They conclude that there is insufficient evidence to support the more vigorous approaches to treatment of myopia, and provide some useful explantations for patients and their parents who question the ophthalmologist's decision to treat conservatively.", "contents": "Myopia--a treatable \"disease\"? The authors cite various information and misinformation regarding proposed treatments (atropinization, contact lenses, orthokeratology, wearing glasses, not wearing glasses) for the progression of myopia. They conclude that there is insufficient evidence to support the more vigorous approaches to treatment of myopia, and provide some useful explantations for patients and their parents who question the ophthalmologist's decision to treat conservatively.", "PMID": 959999}
{"id": "pubmed23n0032_3749", "title": "Long-term follow-up of peptic strictures managed by dilatation, modified Collis gastroplasty, and Belsey hiatus hernia repair.", "content": "Between 1964 and 1974, 277 patients with peptic esophagitis were managed by modified Collis gastroplasty and Belsey hiatus hernia repair. By adding a gastroplasty in patients with esophageal shortening, an antireflux repair can be done below the diaphragm, with elimination of tension on both the repair and the intrathoracic esophagus. Indications for repair in this series were peptic strictures, 102; recurrent hiatus hernia, 90; panmural esophagitis with stricture, 44; and reflux esophagitis associated with primary motor disorders, 41. Results of treatment are being evaluated by clinical history, esophagography, esophagoscopy and manometry; and generally they appear to be excellent. However, follow-up is too short in many of these patients to permit meaningful evaluation. A more critical analysis is provided by long-term follow-up of patients with the most severe pathology. This report reviews results in the 33 patients in the series, with peptic strictures, operated on more than 5 years ago. Five of the 33 patients died of unrelated disease before reaching their fifth year after operation, and two were lost to followup. Twenty-six patients have been followed 5 to 12 years since operation. Twenty-five patients had excellent results which were sustained during the period of follow-up. They take a regular diet without dysphagia, and none has symptomatic reflux. One patient, whose symptoms initially resolved, developed recurrent reflux due to peptic ulceration and pyloric stenosis. The functional results achieved with this operation are good and are maintained well beyond 5 years. Results reported with alternative, conservative operations for peptic stricture are reviewed.", "contents": "Long-term follow-up of peptic strictures managed by dilatation, modified Collis gastroplasty, and Belsey hiatus hernia repair. Between 1964 and 1974, 277 patients with peptic esophagitis were managed by modified Collis gastroplasty and Belsey hiatus hernia repair. By adding a gastroplasty in patients with esophageal shortening, an antireflux repair can be done below the diaphragm, with elimination of tension on both the repair and the intrathoracic esophagus. Indications for repair in this series were peptic strictures, 102; recurrent hiatus hernia, 90; panmural esophagitis with stricture, 44; and reflux esophagitis associated with primary motor disorders, 41. Results of treatment are being evaluated by clinical history, esophagography, esophagoscopy and manometry; and generally they appear to be excellent. However, follow-up is too short in many of these patients to permit meaningful evaluation. A more critical analysis is provided by long-term follow-up of patients with the most severe pathology. This report reviews results in the 33 patients in the series, with peptic strictures, operated on more than 5 years ago. Five of the 33 patients died of unrelated disease before reaching their fifth year after operation, and two were lost to followup. Twenty-six patients have been followed 5 to 12 years since operation. Twenty-five patients had excellent results which were sustained during the period of follow-up. They take a regular diet without dysphagia, and none has symptomatic reflux. One patient, whose symptoms initially resolved, developed recurrent reflux due to peptic ulceration and pyloric stenosis. The functional results achieved with this operation are good and are maintained well beyond 5 years. Results reported with alternative, conservative operations for peptic stricture are reviewed.", "PMID": 960006}
{"id": "pubmed23n0032_3750", "title": "Teratogenicity studies with methotrexate, aminopterin, and acetylsalicylic acid in domestic cats.", "content": "Pregnancy was timed in cats following induced ovulation. Methotrexate, (0.5 mg/kg), aminopterin, (0.1 mg/kg), and acetylsalicylic acid, (25 or 50 mg/kg) were administered orally in gelatin capsules in single daily doses on different days of gestation, methotrexate (MTX) on days 11-14, 14-17, or 17-20, aminopterin on day 12, 14, or 16, and acetylsalicylic acid (ASA) on days 10-15 or 15-20. Maternal toxicity was produced only by MTX. MTX given on days 11-14 and 14-17 produced high frequencies of malformations including umbilical hernia. Aminopterin caused no conclusive teratogenic response. An overall increased frequency of anomalies occurred after 50 mg/kg ASA but no single anomaly predominated.", "contents": "Teratogenicity studies with methotrexate, aminopterin, and acetylsalicylic acid in domestic cats. Pregnancy was timed in cats following induced ovulation. Methotrexate, (0.5 mg/kg), aminopterin, (0.1 mg/kg), and acetylsalicylic acid, (25 or 50 mg/kg) were administered orally in gelatin capsules in single daily doses on different days of gestation, methotrexate (MTX) on days 11-14, 14-17, or 17-20, aminopterin on day 12, 14, or 16, and acetylsalicylic acid (ASA) on days 10-15 or 15-20. Maternal toxicity was produced only by MTX. MTX given on days 11-14 and 14-17 produced high frequencies of malformations including umbilical hernia. Aminopterin caused no conclusive teratogenic response. An overall increased frequency of anomalies occurred after 50 mg/kg ASA but no single anomaly predominated.", "PMID": 960008}
{"id": "pubmed23n0032_3751", "title": "Test of the hypothesis that embryonic face shape is a causal factor in genetic predisposition to cleft lip in mice.", "content": "It was proposed that embryonic face shape is one of the quantitative variables underlying the threshold trait, cleft lip. To test the hypothesis several facial dimensions were measured in photographs of embryos at an early stage of formation of the face in 3 lines of mice. The lines had been selected from one original population using frequency of cleft lip induced by 6-aminonicotinamide as the selection criterion. Line L was susceptible to spontaneous cleft lip; lines M and C were not. Line L had a significantly smaller distance between the nasal pits than the other 2 lines, but did not differ in overall head size. A trend towards reduced angle between the medial nasal processes in the L line was noted, though this was not statistically significant. These results were predicted by the face-shape hypothesis.", "contents": "Test of the hypothesis that embryonic face shape is a causal factor in genetic predisposition to cleft lip in mice. It was proposed that embryonic face shape is one of the quantitative variables underlying the threshold trait, cleft lip. To test the hypothesis several facial dimensions were measured in photographs of embryos at an early stage of formation of the face in 3 lines of mice. The lines had been selected from one original population using frequency of cleft lip induced by 6-aminonicotinamide as the selection criterion. Line L was susceptible to spontaneous cleft lip; lines M and C were not. Line L had a significantly smaller distance between the nasal pits than the other 2 lines, but did not differ in overall head size. A trend towards reduced angle between the medial nasal processes in the L line was noted, though this was not statistically significant. These results were predicted by the face-shape hypothesis.", "PMID": 960009}
{"id": "pubmed23n0032_3752", "title": "The teratogenic effects of beta-aminopropionitrile in hamsters.", "content": "The teratogenicity of beta-aminopropionitrile (beta APN) was evaluated in golden Syrian hamsters; single doses of 1250, 2500, and 3750 mg/kg given on days 7-13 produced fetal mortality, growth retardation and malformations. All time-dose combinations produced gross external abnormalities with the highest rate (92.5%) occurring following day-12 treatment. High frequencies of skeletal abnormalities including deformities of the ribs, fibula, and scapula occurred after days-10-12 treatment. Deformed ribs occurred in 100% of fetuses exposed on day 11 to any of the dosages. The types of abnormality generally resembled those produced by lathyrogens in other species except for exencephaly and encephalocele, which occurred after treatment on day 7. These results indicate that betaAPN is a typical teratogen, capable of producing abnormalities of the central nervous system early in gestation, and may reflect a broader mechanism for its activity than previously suggested.", "contents": "The teratogenic effects of beta-aminopropionitrile in hamsters. The teratogenicity of beta-aminopropionitrile (beta APN) was evaluated in golden Syrian hamsters; single doses of 1250, 2500, and 3750 mg/kg given on days 7-13 produced fetal mortality, growth retardation and malformations. All time-dose combinations produced gross external abnormalities with the highest rate (92.5%) occurring following day-12 treatment. High frequencies of skeletal abnormalities including deformities of the ribs, fibula, and scapula occurred after days-10-12 treatment. Deformed ribs occurred in 100% of fetuses exposed on day 11 to any of the dosages. The types of abnormality generally resembled those produced by lathyrogens in other species except for exencephaly and encephalocele, which occurred after treatment on day 7. These results indicate that betaAPN is a typical teratogen, capable of producing abnormalities of the central nervous system early in gestation, and may reflect a broader mechanism for its activity than previously suggested.", "PMID": 960010}
{"id": "pubmed23n0032_3753", "title": "Transplacental passage of label after administration of (3H) retinoic acid (vitamin A acid) to pregnant mice.", "content": "To determine whether there is transplacental passage of retinoic acid (vitamin A acid) or its metabolites levels of radioactivity were measured in embryos after administration to 12th-day pregnant ICR/DUB mice of nonteratogenic or teratogenic quantities of (3H)retinoic acid. After the former treatment radioactivity was demonstrable in embryos, placentas, and visceral yolk sacs (VYS) at 1 h after treatment. Expressed in terms of radioactivity per unit protein content, embryos at 1 h had about one-third as much radioactivity as maternal livers. The placentas had maximum radioactivity at 2 h, whereas embryos and VYS accumulated maximum levels by 6 h after treatment. At 6 h the level in embryos was twice that in placentas. After teratogenic treatment placentas and embryos attained peak levels of radioactivity at 6 and 12 h later, respectively. Again, embryos at peak level contained 2-3 times as much radioactivity as placentas or VYS. The amount of retinoic acid, metabolites, or both, in embryos after the teratogenic treatment, calculated on the basis of specific activity of the injected label, was 26 times greater than after nonteratogenic treatment; the amount in maternal liver was 75 times greater. The results support previous evidence that the teratogenic effects of retinoic acid are associated with raised levels of retinoic acid, its metabolites, or both, in embryos.", "contents": "Transplacental passage of label after administration of (3H) retinoic acid (vitamin A acid) to pregnant mice. To determine whether there is transplacental passage of retinoic acid (vitamin A acid) or its metabolites levels of radioactivity were measured in embryos after administration to 12th-day pregnant ICR/DUB mice of nonteratogenic or teratogenic quantities of (3H)retinoic acid. After the former treatment radioactivity was demonstrable in embryos, placentas, and visceral yolk sacs (VYS) at 1 h after treatment. Expressed in terms of radioactivity per unit protein content, embryos at 1 h had about one-third as much radioactivity as maternal livers. The placentas had maximum radioactivity at 2 h, whereas embryos and VYS accumulated maximum levels by 6 h after treatment. At 6 h the level in embryos was twice that in placentas. After teratogenic treatment placentas and embryos attained peak levels of radioactivity at 6 and 12 h later, respectively. Again, embryos at peak level contained 2-3 times as much radioactivity as placentas or VYS. The amount of retinoic acid, metabolites, or both, in embryos after the teratogenic treatment, calculated on the basis of specific activity of the injected label, was 26 times greater than after nonteratogenic treatment; the amount in maternal liver was 75 times greater. The results support previous evidence that the teratogenic effects of retinoic acid are associated with raised levels of retinoic acid, its metabolites, or both, in embryos.", "PMID": 960011}
{"id": "pubmed23n0032_3754", "title": "In vitro development of postimplantation rat embryos cultured on dialyzed rat serum.", "content": "Attempts to culture postimplantation rat embryos on defined media have not been successful although they grow well when cultured on homologous serum. As a first step in the search for factors in serum that support growth and differentiation of such cultured preparations the following experiments were undertaken. Six-somite rat embryos were cultured on whole serum, dialyzed serum, or the buffered salt solution (BSS) used for dialysis. Additional experiments were conducted utilizing BSS supplemented with glucose or dialyzed serum supplemented with glucose, mannose, fructose, or pyruvate. Of the media tested only glucose-supplemented dialyzed serum maintained development at a level comparable to that obtained with whole serum. Further preliminary studies with combined supplementation and metabolic poisoning suggested that anaerobic glycolysis is essential for the in-vitro growth and differentiation of these preparations.", "contents": "In vitro development of postimplantation rat embryos cultured on dialyzed rat serum. Attempts to culture postimplantation rat embryos on defined media have not been successful although they grow well when cultured on homologous serum. As a first step in the search for factors in serum that support growth and differentiation of such cultured preparations the following experiments were undertaken. Six-somite rat embryos were cultured on whole serum, dialyzed serum, or the buffered salt solution (BSS) used for dialysis. Additional experiments were conducted utilizing BSS supplemented with glucose or dialyzed serum supplemented with glucose, mannose, fructose, or pyruvate. Of the media tested only glucose-supplemented dialyzed serum maintained development at a level comparable to that obtained with whole serum. Further preliminary studies with combined supplementation and metabolic poisoning suggested that anaerobic glycolysis is essential for the in-vitro growth and differentiation of these preparations.", "PMID": 960012}
{"id": "pubmed23n0032_3755", "title": "Studies of the etiology of thalidomide dysmorphogenesis.", "content": "Thalidomide was administered to pregnant rabbits in dosages of 150-250 mg/kg/day on days 8-12 of gestation. These females produced 40 offspring, 21 of which were deformed. Four control females produced 34 offspring, none of which was deformed. The C6 and C7 ganglia of day-13, -15, -17, and -21 control and experimental embryos and fetuses were examined electron microscopically. Degenerative changes were found in the neurons and axons of dorsal root ganglia in day-13 experimental embryos, i.e., at least 16h before the earliest signs of thalidomide dysmelia have been reported in rabbits  (Vickers, '67). Since the dorsal root ganglia form in rabbits on days 11 and 12 the changes evident at day 13 indicate that degeneration of neurons and axons may be a pathogenetic factor in thalidomide-induced peripheral deformities.", "contents": "Studies of the etiology of thalidomide dysmorphogenesis. Thalidomide was administered to pregnant rabbits in dosages of 150-250 mg/kg/day on days 8-12 of gestation. These females produced 40 offspring, 21 of which were deformed. Four control females produced 34 offspring, none of which was deformed. The C6 and C7 ganglia of day-13, -15, -17, and -21 control and experimental embryos and fetuses were examined electron microscopically. Degenerative changes were found in the neurons and axons of dorsal root ganglia in day-13 experimental embryos, i.e., at least 16h before the earliest signs of thalidomide dysmelia have been reported in rabbits  (Vickers, '67). Since the dorsal root ganglia form in rabbits on days 11 and 12 the changes evident at day 13 indicate that degeneration of neurons and axons may be a pathogenetic factor in thalidomide-induced peripheral deformities.", "PMID": 960013}
{"id": "pubmed23n0032_3756", "title": "Neural-tube defects caused by 5-bromodeoxyuridine in chicks.", "content": "Chick embryos were explanted at HH stage 4 and cultured for 24 h on thin albumen with 10 mug/ml 5-bromodeoxyuridine (BrdU). BrdU inhibited closure of the neural tube in over 90% of the embryos. Mitotic figures were found throughout adversely effected neuroepithelium, suggesting that interkinetic nuclear migration had been inhibited. Electron microscopic studies of BrdU-treated neural-tube cells showed fewer and more amorphous cytoplasmic extensions, microfilaments, and demosomal (juctional) tonofibrils than in controls, but microtubules were unaffected.", "contents": "Neural-tube defects caused by 5-bromodeoxyuridine in chicks. Chick embryos were explanted at HH stage 4 and cultured for 24 h on thin albumen with 10 mug/ml 5-bromodeoxyuridine (BrdU). BrdU inhibited closure of the neural tube in over 90% of the embryos. Mitotic figures were found throughout adversely effected neuroepithelium, suggesting that interkinetic nuclear migration had been inhibited. Electron microscopic studies of BrdU-treated neural-tube cells showed fewer and more amorphous cytoplasmic extensions, microfilaments, and demosomal (juctional) tonofibrils than in controls, but microtubules were unaffected.", "PMID": 960014}
{"id": "pubmed23n0032_3757", "title": "[Some forms of disease associated with impaired locomotion in sheep (author's transl)].", "content": "A number of flock diseases in sheep associated with impaired locomotion, which are of importance or may assume importance in the Netherlands, are reviewed. Attention is paid to the following metabolic disorders and deficiencies: acetonaemia, hypocalcaemia, hypomagnesaemia, some forms of osteopathy, enzootic ataxia and cerebrocortical necrosis. The following forms of infectious diseases are described: listeric encephalitis, purulent meningitis following injury to the throat, scrapie, visna, Aujeszky's disease and border disease. Finally, a number of viral forms of encephalitis are discussed, which are of minor importance or negligible in the Netherlands, though they are of importance in neighbouring countries. No attempt was made exhaustively to describe the above diseases. The aetiology and pathogenesis usually were not discussed in greater detail than that required for the diagnosis, treatment and prevention of the disease. Efforts were made to be of some help to the veterinary practitioner in controlling some forms of ovine disease in cases in which this is necessary and possible.", "contents": "[Some forms of disease associated with impaired locomotion in sheep (author's transl)]. A number of flock diseases in sheep associated with impaired locomotion, which are of importance or may assume importance in the Netherlands, are reviewed. Attention is paid to the following metabolic disorders and deficiencies: acetonaemia, hypocalcaemia, hypomagnesaemia, some forms of osteopathy, enzootic ataxia and cerebrocortical necrosis. The following forms of infectious diseases are described: listeric encephalitis, purulent meningitis following injury to the throat, scrapie, visna, Aujeszky's disease and border disease. Finally, a number of viral forms of encephalitis are discussed, which are of minor importance or negligible in the Netherlands, though they are of importance in neighbouring countries. No attempt was made exhaustively to describe the above diseases. The aetiology and pathogenesis usually were not discussed in greater detail than that required for the diagnosis, treatment and prevention of the disease. Efforts were made to be of some help to the veterinary practitioner in controlling some forms of ovine disease in cases in which this is necessary and possible.", "PMID": 960067}
{"id": "pubmed23n0032_3758", "title": "[The incidence of diseases of the lung and liver in slaughtered pigs as a criterion in the detection of herds in which the disease is a recurrent problem (author's transl)].", "content": "Diseases of the lung and liver in swine are two forms of disease which often result in considerable economic losses. They occur in pigs throughout the world. Studies in the Netherlands showed that from 40 to 50 per cent of the lungs and from 60 to 80 per cent of the livers were affected. In the Animal Health Service of the province of North Brabant, it was found to be possible to differentiate between pig-fattening farms and piggeries on these farms (a differentiation based on the severity of the disease) by recording the incidence of diseases of the lung and liver in slaughtered pigs. Ever since April 1, 1975, the number of cases of disease of the lung and liver among all slaughtered pigs (approximately 1,000,000 pigs per annum) are recorded in the slaughter-house of the Meat and Cattle Centre in Boxtel as a routine measure. The method of examination adopted in the slaughter-house is described. The results of investigations during the second, third and fourth quarters of 1975 are discussed. A total number of 653,540 pigs were studied. Severe disease of the lung was observed in 20.2 per cent of the swine. Of the livers, 13 per cent were severely affected and 3.3 per cent were entirely condemned. Pleurisy was found to be present in 3.1 per cent of the pigs.", "contents": "[The incidence of diseases of the lung and liver in slaughtered pigs as a criterion in the detection of herds in which the disease is a recurrent problem (author's transl)]. Diseases of the lung and liver in swine are two forms of disease which often result in considerable economic losses. They occur in pigs throughout the world. Studies in the Netherlands showed that from 40 to 50 per cent of the lungs and from 60 to 80 per cent of the livers were affected. In the Animal Health Service of the province of North Brabant, it was found to be possible to differentiate between pig-fattening farms and piggeries on these farms (a differentiation based on the severity of the disease) by recording the incidence of diseases of the lung and liver in slaughtered pigs. Ever since April 1, 1975, the number of cases of disease of the lung and liver among all slaughtered pigs (approximately 1,000,000 pigs per annum) are recorded in the slaughter-house of the Meat and Cattle Centre in Boxtel as a routine measure. The method of examination adopted in the slaughter-house is described. The results of investigations during the second, third and fourth quarters of 1975 are discussed. A total number of 653,540 pigs were studied. Severe disease of the lung was observed in 20.2 per cent of the swine. Of the livers, 13 per cent were severely affected and 3.3 per cent were entirely condemned. Pleurisy was found to be present in 3.1 per cent of the pigs.", "PMID": 960068}
{"id": "pubmed23n0032_3759", "title": "HLA antigens and immunoresponsiveness to insulin in insulin-dependent diabetes mellitus.", "content": "HLA-A and B antigens were determined in 112 patients with insulin-dependent juvenile onset diabetes mellitus, who could be subdivided into \"non\" and \"high responder\" to insulin. The data revealed a trend of an association of these diabetes subgroups with only one of the diabetes-associated antigens HLA-B8 and HLA-BW15 and indicated the existence of at least two different genetic constellations for susceptibility to juvenile diabetes mellitus. One form with a strong immune-response to insulin seemed to be associated with HLA-BW 15 and the other form without humoral immunoreactivity to insulin seemed to be associated with the presence of HLA-B8 and the absence of HLA-B7.", "contents": "HLA antigens and immunoresponsiveness to insulin in insulin-dependent diabetes mellitus. HLA-A and B antigens were determined in 112 patients with insulin-dependent juvenile onset diabetes mellitus, who could be subdivided into \"non\" and \"high responder\" to insulin. The data revealed a trend of an association of these diabetes subgroups with only one of the diabetes-associated antigens HLA-B8 and HLA-BW15 and indicated the existence of at least two different genetic constellations for susceptibility to juvenile diabetes mellitus. One form with a strong immune-response to insulin seemed to be associated with HLA-BW 15 and the other form without humoral immunoreactivity to insulin seemed to be associated with the presence of HLA-B8 and the absence of HLA-B7.", "PMID": 960073}
{"id": "pubmed23n0032_3760", "title": "Some parameters of lymphocyte antibody activity through pregnancy and further eluates of placental material.", "content": "One hundred and sixty-nine pregnancies with lymphocyte cytotoxic antibody activity were found out of 851 full term pregnancies. A rise in incidence of antibody activity was found through first, second and third pregnancies. The average scores for antibody titre and avidity were similar for antibodies to the A and B loci in first, second and third pregnancies though individual scores varied greatly. Five cases are reported where antibody activity was not detectable till the 6-month post-natal stage. ABO compatibility did not have any effect on antibody production. A further 40 placental eluates are reported with the same findings as reported previously.", "contents": "Some parameters of lymphocyte antibody activity through pregnancy and further eluates of placental material. One hundred and sixty-nine pregnancies with lymphocyte cytotoxic antibody activity were found out of 851 full term pregnancies. A rise in incidence of antibody activity was found through first, second and third pregnancies. The average scores for antibody titre and avidity were similar for antibodies to the A and B loci in first, second and third pregnancies though individual scores varied greatly. Five cases are reported where antibody activity was not detectable till the 6-month post-natal stage. ABO compatibility did not have any effect on antibody production. A further 40 placental eluates are reported with the same findings as reported previously.", "PMID": 960074}
{"id": "pubmed23n0032_3761", "title": "Interrelationship between sugar-evoked increases in transmural potential difference and sugar influxes across the mucosal border in the small intestine.", "content": "The relationship between changes in the transmural potential induced by actively transported sugars(deltaPDs) and influxes of the sugars(Js's) was investigated in the isolated guinea pig small intestine. Under the condition that medium electrical conductivity. remains unchanged, deltaPD and Js were closely correlated, e.g., both showed very similar patterns of regional difference along the intestine, and the values of the half saturation concentration (Km) for a sugar estimated from electrical and flux measurements were nearly identical. No such correlation was observed when medium conductivity varied. However, a simple correction of the sizes of deltaPDs for medium conductivity restored a close parallelism between deltaPDs and Js's. The sugar-induced increase in Na+ flux estimated from the increase in short-circuit current varied in parallel with Js regardless of the medium conductivity, and a fixed stoichiometrical relationship (approximately one-to-one coupling) was seen between Na+ and sugar fluxes. It is concluded that the dominant factor affecting the interrelation between deltaPD and Js is the resistance of the paracellular shunt which is directly proportional to medium electrical conductivity", "contents": "Interrelationship between sugar-evoked increases in transmural potential difference and sugar influxes across the mucosal border in the small intestine. The relationship between changes in the transmural potential induced by actively transported sugars(deltaPDs) and influxes of the sugars(Js's) was investigated in the isolated guinea pig small intestine. Under the condition that medium electrical conductivity. remains unchanged, deltaPD and Js were closely correlated, e.g., both showed very similar patterns of regional difference along the intestine, and the values of the half saturation concentration (Km) for a sugar estimated from electrical and flux measurements were nearly identical. No such correlation was observed when medium conductivity varied. However, a simple correction of the sizes of deltaPDs for medium conductivity restored a close parallelism between deltaPDs and Js's. The sugar-induced increase in Na+ flux estimated from the increase in short-circuit current varied in parallel with Js regardless of the medium conductivity, and a fixed stoichiometrical relationship (approximately one-to-one coupling) was seen between Na+ and sugar fluxes. It is concluded that the dominant factor affecting the interrelation between deltaPD and Js is the resistance of the paracellular shunt which is directly proportional to medium electrical conductivity", "PMID": 960090}
{"id": "pubmed23n0032_3762", "title": "Preventive procedure of dysuria after radical hysterectomy by adnexal flap fixation to the bladder.", "content": "To prevent postoperative dysuria, which occurs inevitably after the radical hysterectomy, several surgical procedures have been tried. The principal method is to suture adnexal flaps to the bladder trigone and fundus. Of four procedures so far tried sequentially, the best one was to fix the bladder trigone and to support it with round ligament flaps sutured with the bladder fundus covered by tubal flaps (Type IV). In follow-up studies, Type IV proved more preferable than the other three types or the non-sutured control group in regard to the following aspects: (1) days necessary for the disappearance of residual urine, (2) appearance of urinary sensation, (3) acquirement of urinary sensation, (4) incidence of urinary incontinence, (5) residual urine/bladder capacity ratio, (6) cystometric findings, and others. Effectiveness of our procedures, particularly of Type IV, may be ascribed to the supported bladder trigone and fundus by sutured tissues and to the acquirement of urinary sensation, rather than to the restoration of nervous contact between the bladder and the micturition center in the spinal cord.", "contents": "Preventive procedure of dysuria after radical hysterectomy by adnexal flap fixation to the bladder. To prevent postoperative dysuria, which occurs inevitably after the radical hysterectomy, several surgical procedures have been tried. The principal method is to suture adnexal flaps to the bladder trigone and fundus. Of four procedures so far tried sequentially, the best one was to fix the bladder trigone and to support it with round ligament flaps sutured with the bladder fundus covered by tubal flaps (Type IV). In follow-up studies, Type IV proved more preferable than the other three types or the non-sutured control group in regard to the following aspects: (1) days necessary for the disappearance of residual urine, (2) appearance of urinary sensation, (3) acquirement of urinary sensation, (4) incidence of urinary incontinence, (5) residual urine/bladder capacity ratio, (6) cystometric findings, and others. Effectiveness of our procedures, particularly of Type IV, may be ascribed to the supported bladder trigone and fundus by sutured tissues and to the acquirement of urinary sensation, rather than to the restoration of nervous contact between the bladder and the micturition center in the spinal cord.", "PMID": 960091}
{"id": "pubmed23n0032_3763", "title": "Diagnosis and prognosis of early cancer of the colon and rectum.", "content": "Diagnostic methods for the cancer of the colon and rectum  were compared in two groups of patients, i.e., patients of stage A malignancy in the Dukes classification, and those with the so-called \"early cancer of the colon and rectum\", and the best way to find cancer of the colon and rectum in its early stage was investigated. At the same time, the prognosis of early cancer of the colon and rectum was studied by the use of the relative survival rate. The following results were obtained. 1) The so-called \"early cancer of the colon and rectum\", of which invasion was limited to the mucosa and submucosa, can be correctly diagnosed by the combined application of both biopsy and exfoliative cytology under direct vision even before surgery and/or polypectomy. 2) Cancer of the colon and rectum is considered to be a curable disease, if discovered and resected at or before the stage A in the Dukes classification, in which the cancerous proliferation is limited to the wall of the colon and rectum without any metastasis.", "contents": "Diagnosis and prognosis of early cancer of the colon and rectum. Diagnostic methods for the cancer of the colon and rectum  were compared in two groups of patients, i.e., patients of stage A malignancy in the Dukes classification, and those with the so-called \"early cancer of the colon and rectum\", and the best way to find cancer of the colon and rectum in its early stage was investigated. At the same time, the prognosis of early cancer of the colon and rectum was studied by the use of the relative survival rate. The following results were obtained. 1) The so-called \"early cancer of the colon and rectum\", of which invasion was limited to the mucosa and submucosa, can be correctly diagnosed by the combined application of both biopsy and exfoliative cytology under direct vision even before surgery and/or polypectomy. 2) Cancer of the colon and rectum is considered to be a curable disease, if discovered and resected at or before the stage A in the Dukes classification, in which the cancerous proliferation is limited to the wall of the colon and rectum without any metastasis.", "PMID": 960092}
{"id": "pubmed23n0032_3764", "title": "Analysis of 6,293 routine proctosigmoidoscopies.", "content": "7,679 proctosigmoidoscopies were performed on 6,293 patients during a period from 1962 to 1974 at the Third Department of Internal Medicine, Tohoku University Hospital. Results were summarized as follows: Polyps were observed in 470 patients (7.5%) and cancer was found in 178 patients (2.8%). 129 patients (2.0%) were observed to have ulcerative colitis. There were 57 patients with melanosis coli, 12 radiation proctitis, 7 amebic colitis, 5 granulomatous colitis, 2 endometriosis coli, 2 carcinoid and 2 intestinal tuberculosis. Thus, 879 out of 6,293 patients examined had some forms of disease, an over-all incidence being 14.0%. Biopsies were performed under the proctosigmoidoscopic observation in 82.4% out of 273 patients with polyps during the 6-year period from 1969 to 1974 and 105 patients (46.7%) were observed to have adenomatous polyps. The age-specific distribution pattern and incidences of these patients with adenomatous polyps were mostly consistent with those of 178 patients with cancer of the large bowel, which were observed by proctosigmoidoscopy during the 13-year period from 1962 to 1974. Thus, it is suggested that etiological correlation exists between polyps of adenomatous type and cancer of the large bowel.", "contents": "Analysis of 6,293 routine proctosigmoidoscopies. 7,679 proctosigmoidoscopies were performed on 6,293 patients during a period from 1962 to 1974 at the Third Department of Internal Medicine, Tohoku University Hospital. Results were summarized as follows: Polyps were observed in 470 patients (7.5%) and cancer was found in 178 patients (2.8%). 129 patients (2.0%) were observed to have ulcerative colitis. There were 57 patients with melanosis coli, 12 radiation proctitis, 7 amebic colitis, 5 granulomatous colitis, 2 endometriosis coli, 2 carcinoid and 2 intestinal tuberculosis. Thus, 879 out of 6,293 patients examined had some forms of disease, an over-all incidence being 14.0%. Biopsies were performed under the proctosigmoidoscopic observation in 82.4% out of 273 patients with polyps during the 6-year period from 1969 to 1974 and 105 patients (46.7%) were observed to have adenomatous polyps. The age-specific distribution pattern and incidences of these patients with adenomatous polyps were mostly consistent with those of 178 patients with cancer of the large bowel, which were observed by proctosigmoidoscopy during the 13-year period from 1962 to 1974. Thus, it is suggested that etiological correlation exists between polyps of adenomatous type and cancer of the large bowel.", "PMID": 960093}
{"id": "pubmed23n0032_3765", "title": "Peripherally and centrally mediated bradycardiac effects of clonidine in anesthetized or spinal rats.", "content": "The bradycardia-inducing  effects of clonidine were examined in anesthetized or spinal rats by injecting the drug intracisternally (i.c.) or intravenously (i.v.). Clonidine (1-25mug i.c.) caused a bradycardia dose-dependently in anesthetized rats. The bradycardia in response to clonidine (5mug i.c.) was significantly reduced after a treatment with phentolamine (100 mug i.c.), but not influenced with atropine (1 mg i.v.) or sectioning bilateral cervical vagal nerves. In spinal rats, an acceleration in heart rate by electrical stimulation of cervical sympathetic nerves was frequency-dependent and that due to desmethylimipramine (DMI) was dose-dependent. Clonidine (30 mug i.v.) significantly inhibited the acceleration induced by electrical stimulations only at a low frequency (0.3-3 Hz) or DMI (0.3 mg i.v.). This inhibition by clonidine was antagonized by phentolamine (5 mg i.v.). Clonidine (30 mug i.v.) did not significantly influence the acceleration in heart rate of spinal rats induced by norepinephrine (1 mug i.v.), tyramine (100 mug i.v.) or 1,1-dimethyl-4-phenylpiperazinium (DMPP, 50 mug i.v.). Therefore, it is suggested that clonidine causes a bradycardia by stimulating both peripheral and central alpha-adrenoceptors, the sympathetic trunk is the main pathway, and that the peripheral mechanism for clonidine-induced bradycardia is different from the action of guanethidine or hexamethonium on a release of catecholamines from the cardiac nerve terminals.", "contents": "Peripherally and centrally mediated bradycardiac effects of clonidine in anesthetized or spinal rats. The bradycardia-inducing  effects of clonidine were examined in anesthetized or spinal rats by injecting the drug intracisternally (i.c.) or intravenously (i.v.). Clonidine (1-25mug i.c.) caused a bradycardia dose-dependently in anesthetized rats. The bradycardia in response to clonidine (5mug i.c.) was significantly reduced after a treatment with phentolamine (100 mug i.c.), but not influenced with atropine (1 mg i.v.) or sectioning bilateral cervical vagal nerves. In spinal rats, an acceleration in heart rate by electrical stimulation of cervical sympathetic nerves was frequency-dependent and that due to desmethylimipramine (DMI) was dose-dependent. Clonidine (30 mug i.v.) significantly inhibited the acceleration induced by electrical stimulations only at a low frequency (0.3-3 Hz) or DMI (0.3 mg i.v.). This inhibition by clonidine was antagonized by phentolamine (5 mg i.v.). Clonidine (30 mug i.v.) did not significantly influence the acceleration in heart rate of spinal rats induced by norepinephrine (1 mug i.v.), tyramine (100 mug i.v.) or 1,1-dimethyl-4-phenylpiperazinium (DMPP, 50 mug i.v.). Therefore, it is suggested that clonidine causes a bradycardia by stimulating both peripheral and central alpha-adrenoceptors, the sympathetic trunk is the main pathway, and that the peripheral mechanism for clonidine-induced bradycardia is different from the action of guanethidine or hexamethonium on a release of catecholamines from the cardiac nerve terminals.", "PMID": 960094}
{"id": "pubmed23n0032_3766", "title": "Inorganic phosphorus content in human aqueous humor in relation to hours postmortem.", "content": "It appears possible to utilize the inorganic phosphorus content in the aqueous humor for estimation of postmortem interval, especially if the factors relating to the body temperature are known in detail.", "contents": "Inorganic phosphorus content in human aqueous humor in relation to hours postmortem. It appears possible to utilize the inorganic phosphorus content in the aqueous humor for estimation of postmortem interval, especially if the factors relating to the body temperature are known in detail.", "PMID": 960095}
{"id": "pubmed23n0032_3767", "title": "Mobilization of folate from liver into blood stream following histidine injection in rats.", "content": "A marked reticulocyte response following intraperitoneal injections of histidine monohydrochloride was observed in rats which were fed on a folate-free diet immediately after weaning for a period of 20 days or more. This finding was taken as an evidence for mobilization of folate stored in the liver into blood stream following histidine injection.", "contents": "Mobilization of folate from liver into blood stream following histidine injection in rats. A marked reticulocyte response following intraperitoneal injections of histidine monohydrochloride was observed in rats which were fed on a folate-free diet immediately after weaning for a period of 20 days or more. This finding was taken as an evidence for mobilization of folate stored in the liver into blood stream following histidine injection.", "PMID": 960096}
{"id": "pubmed23n0032_3768", "title": "Effects of inhibitors of DNA and protein syntheses on mitogenic factor production by PPD-stimulated guinea pig lymphocytes.", "content": "The effects of inhibitors of DNA synthesis and mitosis and of protein synthesis on mitogenic factor production by lymph node cells from guinea pigs sensitized to tuberculin was studied. All inhibitors used in this study were added to the medium at the beginning of culture. Cytosine arabinoside, vinblastine and mitomycin-C reduced markedly the incorporation of tritiated thymidine in DNA, but had no effect on the production of mitogenic factor. Mitomycin-C at higher concentrations, however, induced the partial reduction of mitogenic factor production. On the other hand, puromycin and cycloheximide, inhibitors of protein synthesis, suppressed markedly the production of mitogenic factor. These results indicate that mitogenic factor production requires protein synthesis, but is independent of DNA synthesis.", "contents": "Effects of inhibitors of DNA and protein syntheses on mitogenic factor production by PPD-stimulated guinea pig lymphocytes. The effects of inhibitors of DNA synthesis and mitosis and of protein synthesis on mitogenic factor production by lymph node cells from guinea pigs sensitized to tuberculin was studied. All inhibitors used in this study were added to the medium at the beginning of culture. Cytosine arabinoside, vinblastine and mitomycin-C reduced markedly the incorporation of tritiated thymidine in DNA, but had no effect on the production of mitogenic factor. Mitomycin-C at higher concentrations, however, induced the partial reduction of mitogenic factor production. On the other hand, puromycin and cycloheximide, inhibitors of protein synthesis, suppressed markedly the production of mitogenic factor. These results indicate that mitogenic factor production requires protein synthesis, but is independent of DNA synthesis.", "PMID": 960097}
{"id": "pubmed23n0032_3769", "title": "Reduction of myocardial reactive hyperemia during oxygen breathing in dogs.", "content": "In 7 open-chest anesthetized mongrel dogs the left anterior descending coronary artery was occluded for 20 sec. Myocardial reactive hyperemia was recorded during air and oxygen breathings. The excess blood flow during the reactive hyperemia was smaller and the duration of the reactive hyperemia was shorter during oxygen breathing than during air breathing. It is suggested that the reduction of oxygen demand during oxygen breathing is a possible reason for the decreased reactive hyperemia and oxygen administration is beneficial in relieving myocardial ischemia.", "contents": "Reduction of myocardial reactive hyperemia during oxygen breathing in dogs. In 7 open-chest anesthetized mongrel dogs the left anterior descending coronary artery was occluded for 20 sec. Myocardial reactive hyperemia was recorded during air and oxygen breathings. The excess blood flow during the reactive hyperemia was smaller and the duration of the reactive hyperemia was shorter during oxygen breathing than during air breathing. It is suggested that the reduction of oxygen demand during oxygen breathing is a possible reason for the decreased reactive hyperemia and oxygen administration is beneficial in relieving myocardial ischemia.", "PMID": 960098}
{"id": "pubmed23n0032_3770", "title": "Measurement of intrahepatic shunted blood flow by ethanol-1-14C method as compared with D-galactose-1-14C method.", "content": "Ethanol-1-14C method for the measurement of intrahepatic shunted blood flow was compared with the method of continuous infusion of D-galactose-1-14C. In controls, in chronic hepatitis, and in hepatic cirrhosis, per cent intrahepatic shunt measured by the ethanol-1-14C- method was about a half or one-third of that measured by the D-galactose-1-14C method. Study of radioactivity-dye concentration ratio of the blood sampled from the inferior vena cava showed that per cent intrahepatic shunt was underestimated by the ethanol-1-14C method because of permeability of ethanol-1-14C through the capillaries. In patients with hepatic carcinoma, in whom the carcinomatous tissue was supplied mainly by the hepatic artery, there was no significant difference in per cent intrahepatic shunt between both methods.", "contents": "Measurement of intrahepatic shunted blood flow by ethanol-1-14C method as compared with D-galactose-1-14C method. Ethanol-1-14C method for the measurement of intrahepatic shunted blood flow was compared with the method of continuous infusion of D-galactose-1-14C. In controls, in chronic hepatitis, and in hepatic cirrhosis, per cent intrahepatic shunt measured by the ethanol-1-14C- method was about a half or one-third of that measured by the D-galactose-1-14C method. Study of radioactivity-dye concentration ratio of the blood sampled from the inferior vena cava showed that per cent intrahepatic shunt was underestimated by the ethanol-1-14C method because of permeability of ethanol-1-14C through the capillaries. In patients with hepatic carcinoma, in whom the carcinomatous tissue was supplied mainly by the hepatic artery, there was no significant difference in per cent intrahepatic shunt between both methods.", "PMID": 960099}
{"id": "pubmed23n0032_3771", "title": "Mercury in cigarettes.", "content": "Mercury in cigarettes of domestic and foreign products was measured by Magon's method, and the sample of a domestic brand was burned by a smoking machine and the smoke was collected for the measurement of mercury. In tobacco leaf and paper, only inorganic mercury was found in both domestic and foreign products. Its amount in tobacco leaf and paper of a piece of cigarette was about 60 ng in domestic products and about 30 ng in foreign products. By burning, the unit length of a cigarette released about 5-7 ng of mercury into smoke.", "contents": "Mercury in cigarettes. Mercury in cigarettes of domestic and foreign products was measured by Magon's method, and the sample of a domestic brand was burned by a smoking machine and the smoke was collected for the measurement of mercury. In tobacco leaf and paper, only inorganic mercury was found in both domestic and foreign products. Its amount in tobacco leaf and paper of a piece of cigarette was about 60 ng in domestic products and about 30 ng in foreign products. By burning, the unit length of a cigarette released about 5-7 ng of mercury into smoke.", "PMID": 960100}
{"id": "pubmed23n0032_3772", "title": "Relationship between insulin secretory function and endogenous hypertriglyceridemia in obese humans with insulin resistance.", "content": "Eighteen obese inpatients with insulin resistance revealed by i.v. insulin test and expressed in various grades of hyperinsulinemia and hyperglycemia were examined for plasma lipid levels. A significant positive correlation was found to be present between the plasma triglyceride (TG) level and the insulin response to glucose load. A stepwise multiple regression analysis revealed that the insulin secretory response, the plasma cholesterol level and the relative body weight contributed to the level of plasma TG. No difference was found in the grades of insulin resistance between patients with and without elevated TG. The ratio of sum of plasma insulin values to that of blood glucose values during glucose tolerance test was markedly increased in patients with elevated TG. The patients with relatively blunted insulin response and impaired glucose tolerance curves showed only slight slight hypertriglyceridemia. Endogenous hypertriglyceridemia in obesity seems to be more closely correlated with plasma insulin level, and therefore, with insulin action rather than insulin resistance.", "contents": "Relationship between insulin secretory function and endogenous hypertriglyceridemia in obese humans with insulin resistance. Eighteen obese inpatients with insulin resistance revealed by i.v. insulin test and expressed in various grades of hyperinsulinemia and hyperglycemia were examined for plasma lipid levels. A significant positive correlation was found to be present between the plasma triglyceride (TG) level and the insulin response to glucose load. A stepwise multiple regression analysis revealed that the insulin secretory response, the plasma cholesterol level and the relative body weight contributed to the level of plasma TG. No difference was found in the grades of insulin resistance between patients with and without elevated TG. The ratio of sum of plasma insulin values to that of blood glucose values during glucose tolerance test was markedly increased in patients with elevated TG. The patients with relatively blunted insulin response and impaired glucose tolerance curves showed only slight slight hypertriglyceridemia. Endogenous hypertriglyceridemia in obesity seems to be more closely correlated with plasma insulin level, and therefore, with insulin action rather than insulin resistance.", "PMID": 960101}
{"id": "pubmed23n0032_3773", "title": "Angiocardiographic findings and morphogenesis of criss-cross heart with situs solitus, concordant artrioventricular relationships and L-transposition.", "content": "A case of so-called criss-cross heart is presented. The right atrium drained to the left-sided morphologic right ventricle from which the aorta originated anteriorly and leftward. The left atrium drained to the right-sided morphologic left ventricle, from which the pulmonary artey originated posteriorly and rightward. These angiographic findings are morphogenetically interpreted as a clockwise rotation of approximate 90 degrees looking toward the base (from the inferior view) of d-bulboventricular loop following the truncal septation.", "contents": "Angiocardiographic findings and morphogenesis of criss-cross heart with situs solitus, concordant artrioventricular relationships and L-transposition. A case of so-called criss-cross heart is presented. The right atrium drained to the left-sided morphologic right ventricle from which the aorta originated anteriorly and leftward. The left atrium drained to the right-sided morphologic left ventricle, from which the pulmonary artey originated posteriorly and rightward. These angiographic findings are morphogenetically interpreted as a clockwise rotation of approximate 90 degrees looking toward the base (from the inferior view) of d-bulboventricular loop following the truncal septation.", "PMID": 960102}
{"id": "pubmed23n0032_3774", "title": "Two cases of left-sided tricuspid atresia with situs solitus L-bulboventricular loop and L-transposition of the great arteries.", "content": "Two cases of extremely rare type of tricuspid atresia are presented. Both possessed left-sided tricuspid atresia, situs solitus of viscera and atria, 1-bulboventricular loop and 1-transposition of the great arteries. These anomalies are typical tricuspid atresia with 1-transpositions. The importance of bulboventricular loop in tricuspid atresia is emphasized because the atretic tricuspid valve is right-sided in the heart with solitus and d-loop, and is left-sided in the heart with solitus and 1-loop.", "contents": "Two cases of left-sided tricuspid atresia with situs solitus L-bulboventricular loop and L-transposition of the great arteries. Two cases of extremely rare type of tricuspid atresia are presented. Both possessed left-sided tricuspid atresia, situs solitus of viscera and atria, 1-bulboventricular loop and 1-transposition of the great arteries. These anomalies are typical tricuspid atresia with 1-transpositions. The importance of bulboventricular loop in tricuspid atresia is emphasized because the atretic tricuspid valve is right-sided in the heart with solitus and d-loop, and is left-sided in the heart with solitus and 1-loop.", "PMID": 960103}
{"id": "pubmed23n0032_3775", "title": "Effects of change in concentration of calcium on myocardial contractility depressed by thiamylal and halothane.", "content": "The effects of change in the concentration of Ca++ on myocardial contractility depressed by thiamylal and halothane were studied in isolated dog heart muscle. The concentration of Ca++ in the bathing medium was increased stepwise from 1.9 to 20.9 mM. An increase in Ca++ concentration resulted in an increase both in net-shortening (delta1) and in maximum velocity of shortening at 0.4 g preload (V'max) which reached the maximum values at 11.4mM of Ca++. A further increase in Ca++ concentration rather decreased delta1 and V'max. The concentration-response relation curves in the presence of thiamylal (3.3 mg%) shifted to the right almost parallel to the control curve, with the peaks at 15.2 mM of Ca++. The result indicates that the depression of myocardial contractility produced by thiamylal is counteracted competitively by Ca++. However, the curves in the presence of halothane (5.0 +/- 0.4 mg%) were kept lower than those of the control, with the peaks at 7.6 mM of Ca++. The result shows that the counteraction of Ca++ to the depression of myocardial contractility was limited in the presence of halothane. A similar tendency was observed in maximum force of isometric contraction (Fm). Moreover, in the presence of halothane, the administration of higher concentrations of Ca++ almost always accompanied \"after-contraction\". From the result obtained and the literature concerned it was suggested that the mechanism for producing the depression of myocardial contractility might be different between thiamylal and halothane in relation to Ca++ movements at the Ca++ storage sites within the myocardium.", "contents": "Effects of change in concentration of calcium on myocardial contractility depressed by thiamylal and halothane. The effects of change in the concentration of Ca++ on myocardial contractility depressed by thiamylal and halothane were studied in isolated dog heart muscle. The concentration of Ca++ in the bathing medium was increased stepwise from 1.9 to 20.9 mM. An increase in Ca++ concentration resulted in an increase both in net-shortening (delta1) and in maximum velocity of shortening at 0.4 g preload (V'max) which reached the maximum values at 11.4mM of Ca++. A further increase in Ca++ concentration rather decreased delta1 and V'max. The concentration-response relation curves in the presence of thiamylal (3.3 mg%) shifted to the right almost parallel to the control curve, with the peaks at 15.2 mM of Ca++. The result indicates that the depression of myocardial contractility produced by thiamylal is counteracted competitively by Ca++. However, the curves in the presence of halothane (5.0 +/- 0.4 mg%) were kept lower than those of the control, with the peaks at 7.6 mM of Ca++. The result shows that the counteraction of Ca++ to the depression of myocardial contractility was limited in the presence of halothane. A similar tendency was observed in maximum force of isometric contraction (Fm). Moreover, in the presence of halothane, the administration of higher concentrations of Ca++ almost always accompanied \"after-contraction\". From the result obtained and the literature concerned it was suggested that the mechanism for producing the depression of myocardial contractility might be different between thiamylal and halothane in relation to Ca++ movements at the Ca++ storage sites within the myocardium.", "PMID": 960104}
{"id": "pubmed23n0032_3776", "title": "Changes in hypophysio-ovarian endocrinological function of post-menopausal and castrated women.", "content": "The serum levels of estradiol, progesterone, LH and FSH were determined by radioimmunoassay in post-menopausal and castrated women and the data obtained at the same periods after menopause and castration were compared. The serum levels of estradiol and progesterone in post-menopausal women within 1 year after menopause were higher than those of castrated women. The result suggested that ovarian function was indeed depressed in the post-menopausal women but was still preserved for 1 to 2 years after menopause. In the post-menopausal women, the levels of serum LH and FSH rose gradually to reach a peak at 1 to 3 years after menopause, when the production of ovarian sex steroid hormones presumably ceased. On the contrary, the serum LH and FSH levels of castrated women began to decrease gradually 4 to 9 years after castration. It is highly suggestive from these findings that a negative feedback mechanism is elicited by ageing.", "contents": "Changes in hypophysio-ovarian endocrinological function of post-menopausal and castrated women. The serum levels of estradiol, progesterone, LH and FSH were determined by radioimmunoassay in post-menopausal and castrated women and the data obtained at the same periods after menopause and castration were compared. The serum levels of estradiol and progesterone in post-menopausal women within 1 year after menopause were higher than those of castrated women. The result suggested that ovarian function was indeed depressed in the post-menopausal women but was still preserved for 1 to 2 years after menopause. In the post-menopausal women, the levels of serum LH and FSH rose gradually to reach a peak at 1 to 3 years after menopause, when the production of ovarian sex steroid hormones presumably ceased. On the contrary, the serum LH and FSH levels of castrated women began to decrease gradually 4 to 9 years after castration. It is highly suggestive from these findings that a negative feedback mechanism is elicited by ageing.", "PMID": 960105}
{"id": "pubmed23n0032_3777", "title": "Malignant lymphoma initiated with malabsorption syndrome due to Isospora belli infection and lymphocytosis.", "content": "A 47-year-old man had diarrhea in 1965. Four years later, malabsorption syndrome was diagnosed and the patient was found to have mild lymphocytosis. Abdominal lymphoma was suspected, but exploratory laparotomy was normal except for partial villous atrophy of small intestine and slightly enlarged mesenteric lymphnodes which were normal microscopically. In vitro lymphocyte blastformation with phytohemagglutinin was depressed markedly throughout the course and the result predicted the developement of malignancy of the lymphocytic system. Infection of Isospora belli was found thereafter, and sulfamethoxazole was quite effective for diarrhea. In August, 1974, he noticed cervical lymphadenopathy for the first time and it was diagnosed as undifferentiated type of malignant lymphoma. He died in December, 1974. In this case diarrhea was most probably caused by the intestinal infection of Isospora belli without obvious lymphoma. The symptom was swept away by peroral sulfamethoxazole. In this patient coccidiosis was presumably induced and prolonged by suppression of cellular immunity which might have already begun to progress at the onset of diarrhea.", "contents": "Malignant lymphoma initiated with malabsorption syndrome due to Isospora belli infection and lymphocytosis. A 47-year-old man had diarrhea in 1965. Four years later, malabsorption syndrome was diagnosed and the patient was found to have mild lymphocytosis. Abdominal lymphoma was suspected, but exploratory laparotomy was normal except for partial villous atrophy of small intestine and slightly enlarged mesenteric lymphnodes which were normal microscopically. In vitro lymphocyte blastformation with phytohemagglutinin was depressed markedly throughout the course and the result predicted the developement of malignancy of the lymphocytic system. Infection of Isospora belli was found thereafter, and sulfamethoxazole was quite effective for diarrhea. In August, 1974, he noticed cervical lymphadenopathy for the first time and it was diagnosed as undifferentiated type of malignant lymphoma. He died in December, 1974. In this case diarrhea was most probably caused by the intestinal infection of Isospora belli without obvious lymphoma. The symptom was swept away by peroral sulfamethoxazole. In this patient coccidiosis was presumably induced and prolonged by suppression of cellular immunity which might have already begun to progress at the onset of diarrhea.", "PMID": 960106}
{"id": "pubmed23n0032_3778", "title": "Evidence for a familial form of hypertriglyceridemia as disorders coupled with insulin resistance.", "content": "To evaluate the role of insulin in familial hypertriglyceridemia, 34 relatives of the pedigrees of 3 index cases of endogenous hypertriglyceridemia and hepatic steatosis as well as 9 spouses were examined for plasma lipids and responses of blood glucose and plasma insulin during oral glucose tolerance tests. The combined disorders of hypertriglyceridemia and hyperinsulinemia plus glucose intolerance--insulin resistance--were most commonly found among the relatives, which were often accompanied by an impaired liver function. Some relatives showed hyperinsulinemia without hypertriglyceridemia. Obesity was frequent, but its incidence was similar to the controls. Thus, the observed form of familial hypertriglyceridemia was apparently coupled with insulin resistance; and hyperinsulinemia, or insulin resistance by itself, might be a basic genetical trait in this form of lipid disorder.", "contents": "Evidence for a familial form of hypertriglyceridemia as disorders coupled with insulin resistance. To evaluate the role of insulin in familial hypertriglyceridemia, 34 relatives of the pedigrees of 3 index cases of endogenous hypertriglyceridemia and hepatic steatosis as well as 9 spouses were examined for plasma lipids and responses of blood glucose and plasma insulin during oral glucose tolerance tests. The combined disorders of hypertriglyceridemia and hyperinsulinemia plus glucose intolerance--insulin resistance--were most commonly found among the relatives, which were often accompanied by an impaired liver function. Some relatives showed hyperinsulinemia without hypertriglyceridemia. Obesity was frequent, but its incidence was similar to the controls. Thus, the observed form of familial hypertriglyceridemia was apparently coupled with insulin resistance; and hyperinsulinemia, or insulin resistance by itself, might be a basic genetical trait in this form of lipid disorder.", "PMID": 960107}
{"id": "pubmed23n0032_3779", "title": "An illuminator for ultramicrotome knife orientation and block approach.", "content": "The construction and operation of a simple, inexpensive illuminator that produces high quality illumination of the ultramicrotome knife edge and the edge to block face gap resembling dark field is described. Use of the illuminator greatly speeds knife adjustment and reduces the likelihood of specimen or knife edge damage. The illuminator uses a grain-of-wheat light bulb and an adjustable bulb holder fashioned from bent paper clips. The holder permits both lateral and axial adjustment of the bulb position, which is necessary to achieve satisfactory illumination with different specimens and knives. The illuminator, with slight modification, can be adapted for use on any ultramicrotome.", "contents": "An illuminator for ultramicrotome knife orientation and block approach. The construction and operation of a simple, inexpensive illuminator that produces high quality illumination of the ultramicrotome knife edge and the edge to block face gap resembling dark field is described. Use of the illuminator greatly speeds knife adjustment and reduces the likelihood of specimen or knife edge damage. The illuminator uses a grain-of-wheat light bulb and an adjustable bulb holder fashioned from bent paper clips. The holder permits both lateral and axial adjustment of the bulb position, which is necessary to achieve satisfactory illumination with different specimens and knives. The illuminator, with slight modification, can be adapted for use on any ultramicrotome.", "PMID": 960128}
{"id": "pubmed23n0032_3780", "title": "[Results of radiotherapy of malignant testicular neoplasms].", "content": "It is very difficult to compare the therapeutic results of malignant testicular tumours because of the different histological classification systems, the uncertainty in the definition of the different stages, the error rate of the lymphography which amounts up to 35%, and the multitude of surgical, radiation, and chemotherapeutic methods. A histological classification and an exact determination of the stage is required as a condition of beginning a radiotherapy. The desirable focal dose for seminomas is between 4000 and 5000 rad, the maximum dose for teratomas is 6000 rad. For the stages T1-3N0 the iliac and the paraaortic lymph nodes are irradiated, for the stages T1-4N1-2 the interpleural space andthe supraclavicular region are included. For a group of 91 patients there was reached a five-year survival rate of 85,5% in case of seminomas and of 64% in case of teratomas.", "contents": "[Results of radiotherapy of malignant testicular neoplasms]. It is very difficult to compare the therapeutic results of malignant testicular tumours because of the different histological classification systems, the uncertainty in the definition of the different stages, the error rate of the lymphography which amounts up to 35%, and the multitude of surgical, radiation, and chemotherapeutic methods. A histological classification and an exact determination of the stage is required as a condition of beginning a radiotherapy. The desirable focal dose for seminomas is between 4000 and 5000 rad, the maximum dose for teratomas is 6000 rad. For the stages T1-3N0 the iliac and the paraaortic lymph nodes are irradiated, for the stages T1-4N1-2 the interpleural space andthe supraclavicular region are included. For a group of 91 patients there was reached a five-year survival rate of 85,5% in case of seminomas and of 64% in case of teratomas.", "PMID": 960129}
{"id": "pubmed23n0032_3781", "title": "[Thyroid malignant neoplasms. Therapeutic results in 305 patients].", "content": "Between 1960 and 1972, 305 patients with malignomas of the thyroid gland were surgically treated, irradiated (megavoltage therapy and/or iodine-131), and treated with thyroid hormone according to principles formerly stated. Differentiated adenocarcinomas (34.4% of all the patients) were observed more frequently in women and younger patients, whereas dedifferentiated carcinomas (33.8%) are found relatively more often in men and in an advanced age. Only in 12% of the cases the tumor was discovered in an early stage; thus, an early diagnosis is much too rare so far. In almost two thirds of the patients a spread of metastases occurred. Of these, 11% grew manifest later than five years following the therapy. Distant metastases from differentiated adenocarcinomas accumulated I-131 in sixty per cent, those from undifferentiated carcinomas still in twenty per cent. Treatment results are influenced decisively by histology, tumor spread, age and sex, women less than forty years old with differentiated adenocarcinomas and a circumscribed extension of the tumor having the best prognosis. More prognostic importance is due to the extension of the primary tumor than to the presence of metastases to regionary lymph nodes. Patients with metastases accumulating I-131 have a better chance of survival than those whose metastases do not accumulate. By means of standardized therapeutic rules, utilizing modern therapeutic techniques and based on interdepartmental cooperation, it was possible to improve the results partly by the two- or threefold as compared to a previously treated group of patients.", "contents": "[Thyroid malignant neoplasms. Therapeutic results in 305 patients]. Between 1960 and 1972, 305 patients with malignomas of the thyroid gland were surgically treated, irradiated (megavoltage therapy and/or iodine-131), and treated with thyroid hormone according to principles formerly stated. Differentiated adenocarcinomas (34.4% of all the patients) were observed more frequently in women and younger patients, whereas dedifferentiated carcinomas (33.8%) are found relatively more often in men and in an advanced age. Only in 12% of the cases the tumor was discovered in an early stage; thus, an early diagnosis is much too rare so far. In almost two thirds of the patients a spread of metastases occurred. Of these, 11% grew manifest later than five years following the therapy. Distant metastases from differentiated adenocarcinomas accumulated I-131 in sixty per cent, those from undifferentiated carcinomas still in twenty per cent. Treatment results are influenced decisively by histology, tumor spread, age and sex, women less than forty years old with differentiated adenocarcinomas and a circumscribed extension of the tumor having the best prognosis. More prognostic importance is due to the extension of the primary tumor than to the presence of metastases to regionary lymph nodes. Patients with metastases accumulating I-131 have a better chance of survival than those whose metastases do not accumulate. By means of standardized therapeutic rules, utilizing modern therapeutic techniques and based on interdepartmental cooperation, it was possible to improve the results partly by the two- or threefold as compared to a previously treated group of patients.", "PMID": 960130}
{"id": "pubmed23n0032_3782", "title": "[Results of radiotherapy of prostatic carcinoma as monotherapy].", "content": "A report is given of the results of histopathologic examinations of subsequent biopsies carried out on 54 patients with prostate carcinomas and of the clinical development of the prostate carcinomas of 89 patients. All these patients were treated only by therapeutic radiology. Then follows a discussion whether the radiation or the hormone therapy has the preponderant values. The possible complications of the two therapy methods are compared.", "contents": "[Results of radiotherapy of prostatic carcinoma as monotherapy]. A report is given of the results of histopathologic examinations of subsequent biopsies carried out on 54 patients with prostate carcinomas and of the clinical development of the prostate carcinomas of 89 patients. All these patients were treated only by therapeutic radiology. Then follows a discussion whether the radiation or the hormone therapy has the preponderant values. The possible complications of the two therapy methods are compared.", "PMID": 960131}
{"id": "pubmed23n0032_3783", "title": "[Clinical relevance of histological differentiation of malignant lymphomas according to the \"Kiel classification\". A retrospective study of correlations in 228 cases from Schleswig-Holstein].", "content": "The correlations between histological type and clinical picture are reported by a retrospective study of 228 cases in Slesvig-Holstein with malignant non-Hodgkin lymphomas who were scheduled by th \"Kiel classification\".", "contents": "[Clinical relevance of histological differentiation of malignant lymphomas according to the \"Kiel classification\". A retrospective study of correlations in 228 cases from Schleswig-Holstein]. The correlations between histological type and clinical picture are reported by a retrospective study of 228 cases in Slesvig-Holstein with malignant non-Hodgkin lymphomas who were scheduled by th \"Kiel classification\".", "PMID": 960132}
{"id": "pubmed23n0032_3784", "title": "[Influence of radiotherapy on lymphocyte stimulation].", "content": "More than 300 lymphocyte cultures of 12 patients with seminomas were examined during the prophylactic radiotherapy and, in several cases, during an extended period until 20.5 months after the end of the treatment. The object of this study was to find out by measuring the capacity of the lymphocytes to be stimulated in vitro wheather they could be damaged by the radiotherapy. Among other reasons, the above mentioned patients were chosen because they had been submitted to irradiations of vast volumes of lymphatic tissues at a uniform focal dose of 4000 rad. The different opinions expressed in the literature (stimulation decreassed resp. increased resp. unchanged) are reflected by our results in such a way that we did not find a qualitative loss of the capacity to be stimulated cultures. The problem of the different opinions about the capacity of lymphocytes to be stimulated after a radiotherapy appears; among other things, to be based on different examination methods. According to these methods- morphological determination of the relative number of lymphoblasts, synthesis of DNA by fluid scintillation counting, or determination of the number of surviving cells in vitro -different results are obtained. It seems not possible to use the lymphocyte stimulation in vitro as a method of testing clinical sideefects occuring during the characteristics of immunity and radiation biology are not differentiated in a more precise manner.", "contents": "[Influence of radiotherapy on lymphocyte stimulation]. More than 300 lymphocyte cultures of 12 patients with seminomas were examined during the prophylactic radiotherapy and, in several cases, during an extended period until 20.5 months after the end of the treatment. The object of this study was to find out by measuring the capacity of the lymphocytes to be stimulated in vitro wheather they could be damaged by the radiotherapy. Among other reasons, the above mentioned patients were chosen because they had been submitted to irradiations of vast volumes of lymphatic tissues at a uniform focal dose of 4000 rad. The different opinions expressed in the literature (stimulation decreassed resp. increased resp. unchanged) are reflected by our results in such a way that we did not find a qualitative loss of the capacity to be stimulated cultures. The problem of the different opinions about the capacity of lymphocytes to be stimulated after a radiotherapy appears; among other things, to be based on different examination methods. According to these methods- morphological determination of the relative number of lymphoblasts, synthesis of DNA by fluid scintillation counting, or determination of the number of surviving cells in vitro -different results are obtained. It seems not possible to use the lymphocyte stimulation in vitro as a method of testing clinical sideefects occuring during the characteristics of immunity and radiation biology are not differentiated in a more precise manner.", "PMID": 960133}
{"id": "pubmed23n0032_3785", "title": "[Sonographic and roentgenologic course controls following radiotherapy of retroperitoneal neoplasms].", "content": "Twelve patients with retroperitoneal tumors were examined sonographically and roentgenologically before and after radiation therapy. The sonograms were evaluated quantitatively by planimetry. Nine cases revealed analogous results in roentgenologic and in sonographic examinations. In three cases sonography revealed a tumor regression which was not verifiable with X-rays. In consideration of these results, sonography is used by us as a primary method for examination of retroperitoneal tumors during the follow-up control.", "contents": "[Sonographic and roentgenologic course controls following radiotherapy of retroperitoneal neoplasms]. Twelve patients with retroperitoneal tumors were examined sonographically and roentgenologically before and after radiation therapy. The sonograms were evaluated quantitatively by planimetry. Nine cases revealed analogous results in roentgenologic and in sonographic examinations. In three cases sonography revealed a tumor regression which was not verifiable with X-rays. In consideration of these results, sonography is used by us as a primary method for examination of retroperitoneal tumors during the follow-up control.", "PMID": 960134}
{"id": "pubmed23n0032_3786", "title": "[Consideration of inhomogeneities in irradiation planning. I. Influence of inhomogeneities on course of depth dose curve in water for cobalt60 gamma rays].", "content": "In radiation therapy, the focal doses during irradiation of a tumor are based on the values for water, since water has almost the same absorption coefficient as muscular tissue, even for different kinds and energies of radiation. But calculation of the tumor dose will become inaccurate if inhomogeneities in the ray path are not considered such as fat, bones, plaster, metal plates, K\u00fcntscher nail, endoprothesis. These materials, having a density phi different from water, represent inhomogeneities relative to water with regard to absorption of high-energy radiations. The experiments yielded the following results: All measurements revealed that the change in the course of the depth dose curve caused by inhomogeneities in water depends essentially on the density phi and on the thickness d of the inhomogeneity. If the density phi of the inhomogeneity exceeds one, a shift of the depth dose curve in water results in direction to the surface: if the density phi is smaller than one, the depth dose curve will move towards greater depth because of the inhomogeneity. With Co-60 gamma radiation, the shift of the depth dose curve in water due to an inhomogeneity occurs almost parallel. A correlation obtained empirically allows a calculation of the extent of the shift, the depth dose is subjected to, for different inhomogeneities. With the density of the inhomogeneity phi2 and the thickness d, the shift V in water (phi1=1) will result from V=0.5 (phi2-1) d (cm) in direction to the surface if phi2 greater than 1, towards greater depth, if phi2 less than 1.", "contents": "[Consideration of inhomogeneities in irradiation planning. I. Influence of inhomogeneities on course of depth dose curve in water for cobalt60 gamma rays]. In radiation therapy, the focal doses during irradiation of a tumor are based on the values for water, since water has almost the same absorption coefficient as muscular tissue, even for different kinds and energies of radiation. But calculation of the tumor dose will become inaccurate if inhomogeneities in the ray path are not considered such as fat, bones, plaster, metal plates, K\u00fcntscher nail, endoprothesis. These materials, having a density phi different from water, represent inhomogeneities relative to water with regard to absorption of high-energy radiations. The experiments yielded the following results: All measurements revealed that the change in the course of the depth dose curve caused by inhomogeneities in water depends essentially on the density phi and on the thickness d of the inhomogeneity. If the density phi of the inhomogeneity exceeds one, a shift of the depth dose curve in water results in direction to the surface: if the density phi is smaller than one, the depth dose curve will move towards greater depth because of the inhomogeneity. With Co-60 gamma radiation, the shift of the depth dose curve in water due to an inhomogeneity occurs almost parallel. A correlation obtained empirically allows a calculation of the extent of the shift, the depth dose is subjected to, for different inhomogeneities. With the density of the inhomogeneity phi2 and the thickness d, the shift V in water (phi1=1) will result from V=0.5 (phi2-1) d (cm) in direction to the surface if phi2 greater than 1, towards greater depth, if phi2 less than 1.", "PMID": 960135}
{"id": "pubmed23n0032_3787", "title": "[Skip technique as a variant of moving field irradiation].", "content": "The skip-technique may be used either for screening or lessening the radiation load to sensitive sound organs or for an adjusted formation of the isodoses or for both. To show the possibilities of application of this elegant and quite practicable method, some examples are reported concerning spondylous processes, para-aortic lymph nodes and tumors in the small pelvis. The loss of radiation dose because of the additional opening and closing procedure of the diaphragm demands a correction of the duration of irradiation.", "contents": "[Skip technique as a variant of moving field irradiation]. The skip-technique may be used either for screening or lessening the radiation load to sensitive sound organs or for an adjusted formation of the isodoses or for both. To show the possibilities of application of this elegant and quite practicable method, some examples are reported concerning spondylous processes, para-aortic lymph nodes and tumors in the small pelvis. The loss of radiation dose because of the additional opening and closing procedure of the diaphragm demands a correction of the duration of irradiation.", "PMID": 960136}
{"id": "pubmed23n0032_3788", "title": "[Inhibition of epidermis proliferation by borderline rays].", "content": "Treatment of guinea pig flanks with very soft X-rays (borderline rays) may stop the uptake of 3H-Tdr during the DNA synthesis phase of epidermal germinative cells. Evidence of increased proliferative activity at any time after the irradiation can only be seen as a symptom of secondary reactive repair processes.", "contents": "[Inhibition of epidermis proliferation by borderline rays]. Treatment of guinea pig flanks with very soft X-rays (borderline rays) may stop the uptake of 3H-Tdr during the DNA synthesis phase of epidermal germinative cells. Evidence of increased proliferative activity at any time after the irradiation can only be seen as a symptom of secondary reactive repair processes.", "PMID": 960137}
{"id": "pubmed23n0032_3789", "title": "[Influence of a preliminary irradiation once or twice on radioresistance of mice*].", "content": "The radioresistance of mice was tested in order to find out in which way it was influence by one or two preliminary irradiations. The thirty-day survival rate after a single total irradiation was taken as a criterion of the radioresistance. Only one test group showed a significant increase of radioresistance after two preliminary irradiations, whereas the resistance of three other groups was reduced remarkably after irradiation doses of 300 resp. 400 R. From these facts we draw the following conclusion: either radiotoxins and the remote effects produced by them are only playing a minor role in NMRI/Han mice or these mice are particular \"low-responders\" as far as the produciton of antibodies against radiotoxins is concerned.", "contents": "[Influence of a preliminary irradiation once or twice on radioresistance of mice*]. The radioresistance of mice was tested in order to find out in which way it was influence by one or two preliminary irradiations. The thirty-day survival rate after a single total irradiation was taken as a criterion of the radioresistance. Only one test group showed a significant increase of radioresistance after two preliminary irradiations, whereas the resistance of three other groups was reduced remarkably after irradiation doses of 300 resp. 400 R. From these facts we draw the following conclusion: either radiotoxins and the remote effects produced by them are only playing a minor role in NMRI/Han mice or these mice are particular \"low-responders\" as far as the produciton of antibodies against radiotoxins is concerned.", "PMID": 960138}
{"id": "pubmed23n0032_3790", "title": "Liver dysfunction following whole-body Co-60 irradiation in gerbil (Meriones Hurrianae Jerdon) and house rat (Rattus rattus Rufescens).", "content": "Liver dysfunction following whole-body Co-60 irradiation has been studied in domestic and desert rat species. A significant elevation in the serum transaminases activity was noticed both in gerbil and house rat. Alkaline phosphatase and plasma cholesterol levels were also increased indicating an early radiation impairment of the liver tissue, which was later confirmed by histological studies. A steady fall in liver glycogen in irradiated gerbils was strikingly in contrast to an increase in irradiated house rat. Drastic depletion in liver glycogen, changes in the serum enzyme levels and the severity of the hepatic necrosis in gerbils point out that desert mammalian species are much more sensitive to radiation hazard as compared with domestic ones.", "contents": "Liver dysfunction following whole-body Co-60 irradiation in gerbil (Meriones Hurrianae Jerdon) and house rat (Rattus rattus Rufescens). Liver dysfunction following whole-body Co-60 irradiation has been studied in domestic and desert rat species. A significant elevation in the serum transaminases activity was noticed both in gerbil and house rat. Alkaline phosphatase and plasma cholesterol levels were also increased indicating an early radiation impairment of the liver tissue, which was later confirmed by histological studies. A steady fall in liver glycogen in irradiated gerbils was strikingly in contrast to an increase in irradiated house rat. Drastic depletion in liver glycogen, changes in the serum enzyme levels and the severity of the hepatic necrosis in gerbils point out that desert mammalian species are much more sensitive to radiation hazard as compared with domestic ones.", "PMID": 960139}
{"id": "pubmed23n0032_3791", "title": "Aplastic anemia in Japanese radiological technicians.", "content": "Among the Japanese radiological technicans, four deaths from aplastic anemia have been reported after 1930. On the other hand, during the period from 1930 to 1960, the population of radiological technicians is estimated to be 74,400 man-years, in which 0.5 aplastic anemias are expected. However, actually three died from aplastic anemia. This difference is statistically significant at the 1% level. While, in the period from 1961 to 1973, the observed value is 1 against 0.7 expected. It is concluded that aplastic anemia had been induced frequently among the Japanese radiological technicians in the era when there was much exposure to occupational radiation.", "contents": "Aplastic anemia in Japanese radiological technicians. Among the Japanese radiological technicans, four deaths from aplastic anemia have been reported after 1930. On the other hand, during the period from 1930 to 1960, the population of radiological technicians is estimated to be 74,400 man-years, in which 0.5 aplastic anemias are expected. However, actually three died from aplastic anemia. This difference is statistically significant at the 1% level. While, in the period from 1961 to 1973, the observed value is 1 against 0.7 expected. It is concluded that aplastic anemia had been induced frequently among the Japanese radiological technicians in the era when there was much exposure to occupational radiation.", "PMID": 960140}
{"id": "pubmed23n0032_3792", "title": "Radiation protection of the patient during radiotherapy.", "content": "Systematic studies of gonadal doses to patients undergoing therapy on 60Co and X-ray machines were carried out using thermoluminescence dosimeters (TLD). Phantom and in vivo measurements were performed for various field sizes and for different positions of the centre of the field on the patient's body with exception of fields including the gonads during radiotherapeutic treatment. It was shown that there is no effect of various SSD and that most radiation reaching gonads is transmitted axially through the body and therefore, it is impossible to reduce this dose with a simple shielding. The figures presented allow prediction of gonadal doses at various conditions. The efficiency of lung, mouth etc. shielding during radiotherapy was also investigated.", "contents": "Radiation protection of the patient during radiotherapy. Systematic studies of gonadal doses to patients undergoing therapy on 60Co and X-ray machines were carried out using thermoluminescence dosimeters (TLD). Phantom and in vivo measurements were performed for various field sizes and for different positions of the centre of the field on the patient's body with exception of fields including the gonads during radiotherapeutic treatment. It was shown that there is no effect of various SSD and that most radiation reaching gonads is transmitted axially through the body and therefore, it is impossible to reduce this dose with a simple shielding. The figures presented allow prediction of gonadal doses at various conditions. The efficiency of lung, mouth etc. shielding during radiotherapy was also investigated.", "PMID": 960141}
{"id": "pubmed23n0032_3793", "title": "Decreased levels of androgen binding protein in the reproductive tract of the restricted (Hre) rat.", "content": "A simple method was developed for assay of androgen binding protein (ABP) in the reproductive tract of male rats. Dextran-coated charcoal was employed to separate bound and free steroid. The number of binding sites quantified by this technique was the same as those determined by other procedures. The ABP levels in testis and epididymis of Hre rats was much lower than those from normal littermates. This abnormality was correlated with defective spermatogenesis in these animals.", "contents": "Decreased levels of androgen binding protein in the reproductive tract of the restricted (Hre) rat. A simple method was developed for assay of androgen binding protein (ABP) in the reproductive tract of male rats. Dextran-coated charcoal was employed to separate bound and free steroid. The number of binding sites quantified by this technique was the same as those determined by other procedures. The ABP levels in testis and epididymis of Hre rats was much lower than those from normal littermates. This abnormality was correlated with defective spermatogenesis in these animals.", "PMID": 960142}
{"id": "pubmed23n0032_3794", "title": "Synthesis of new steroid haptens for radioimmunoassay. Part I. 15beta-Carboxyethylmercaptotestosterone-bovine serum albumin conjugate. Measurement of testosterone in male plasma without chromatography.", "content": "A radioimmunoassay (RIA) procedure has been developed for measurement of testosterone in male plasma after ether:chloroform (4:1) extraction of the plasma sample without resorting to chromatography. The highly specific anti-testosterone serum was generated from both rabbits and sheep immunized with 15beta-carboxyethylmercapto-testosterone-BSA conjugate. The synthesis of 15beta-carboxyethylmercaptotestosterone and the preparation of its BSA conjugate are described. The high affinity (Ka=2.38 X 10(9) liters/mole) antiserum binds 50% of 50 picograms of tritiated testosterone at working dilutions of 1:100,000 to 1:200,000. Both 5alpha and 5beta-dihydrotestosterone compounds exhibited less than 2% cross-reaction. The only other steroids that showed minor cross-reaction were 11beta-hydroxytestosterone (3.8%), progesterone (2.1%), corticosterone (1.6%), and deoxycorticosterone (7.7%).", "contents": "Synthesis of new steroid haptens for radioimmunoassay. Part I. 15beta-Carboxyethylmercaptotestosterone-bovine serum albumin conjugate. Measurement of testosterone in male plasma without chromatography. A radioimmunoassay (RIA) procedure has been developed for measurement of testosterone in male plasma after ether:chloroform (4:1) extraction of the plasma sample without resorting to chromatography. The highly specific anti-testosterone serum was generated from both rabbits and sheep immunized with 15beta-carboxyethylmercapto-testosterone-BSA conjugate. The synthesis of 15beta-carboxyethylmercaptotestosterone and the preparation of its BSA conjugate are described. The high affinity (Ka=2.38 X 10(9) liters/mole) antiserum binds 50% of 50 picograms of tritiated testosterone at working dilutions of 1:100,000 to 1:200,000. Both 5alpha and 5beta-dihydrotestosterone compounds exhibited less than 2% cross-reaction. The only other steroids that showed minor cross-reaction were 11beta-hydroxytestosterone (3.8%), progesterone (2.1%), corticosterone (1.6%), and deoxycorticosterone (7.7%).", "PMID": 960143}
{"id": "pubmed23n0032_3795", "title": "A simple radioimmunoassay for the measurement of testosterone glucosiduronate in unextracted urine.", "content": "A simple, reliable and rapid radioimmunoassay (RIA) for the determination of testosterone glucosiduronate (TG) in crude urine is described. Two protein-TG complexes were investigated in raising antibodies: a) Bovine serum albumin (BSA)-TG and b) human plasma Cohn's fraction IV-4 (CF)-TG. In rabbits, high titers of antibodies were obtained after the injection of CF-TG. The specificity of the antiserum was sufficiently high (cross reaction with free testosterone 27%, with 5alpha-dihydrotestosterone-glucosiduronate 20%). TG was estimated in small aliquots of male and female urine after evaporation overnight at 50 degrees C in order to eliminate interfering material. The intraassay coefficient of variation (CV) was found to be 6% and the interassay CV 11%. TB has been determined in 40 samples of urine simultaneously by \"direct\" RIA and by a \"classical\" RIA following hydrolysis with beta-glucuronidase. The coefficient of correlation was found to be 0.89. The mean excretion of TG in the urine of 26 healthy men amounted to 164+/-51 mug/24 hours with a range from 97 to 346 mug/24 hours. In a group of 16 women a mean urinary excretion of TG of 24+/-10 mug/24 hours was determined. The method allows a technician to assay 40 samples per day.", "contents": "A simple radioimmunoassay for the measurement of testosterone glucosiduronate in unextracted urine. A simple, reliable and rapid radioimmunoassay (RIA) for the determination of testosterone glucosiduronate (TG) in crude urine is described. Two protein-TG complexes were investigated in raising antibodies: a) Bovine serum albumin (BSA)-TG and b) human plasma Cohn's fraction IV-4 (CF)-TG. In rabbits, high titers of antibodies were obtained after the injection of CF-TG. The specificity of the antiserum was sufficiently high (cross reaction with free testosterone 27%, with 5alpha-dihydrotestosterone-glucosiduronate 20%). TG was estimated in small aliquots of male and female urine after evaporation overnight at 50 degrees C in order to eliminate interfering material. The intraassay coefficient of variation (CV) was found to be 6% and the interassay CV 11%. TB has been determined in 40 samples of urine simultaneously by \"direct\" RIA and by a \"classical\" RIA following hydrolysis with beta-glucuronidase. The coefficient of correlation was found to be 0.89. The mean excretion of TG in the urine of 26 healthy men amounted to 164+/-51 mug/24 hours with a range from 97 to 346 mug/24 hours. In a group of 16 women a mean urinary excretion of TG of 24+/-10 mug/24 hours was determined. The method allows a technician to assay 40 samples per day.", "PMID": 960144}
{"id": "pubmed23n0032_3796", "title": "Radioimmunoassays for aldosterone and deoxycorticosterone in plasma and urine.", "content": "Radioimmunoassays for aldosterone and deoxycorticosterone (DOC) are described in which a simple separation procedure using ammonium sulfate stabilization of bound steroid and extraction of free steroid into toluene scintillant allows an \"in vial\" assay without mechanical separation of the two phase system. Extraction and thin layer chromatographic methods for purification of aldosterone and DOC are free of solvent and plate blank effects. Normal values are given for unconjugated aldosterone and DOC in urine, for aldosterone and DOC in plasma and for aldosterone 18-glucuronide in urine.", "contents": "Radioimmunoassays for aldosterone and deoxycorticosterone in plasma and urine. Radioimmunoassays for aldosterone and deoxycorticosterone (DOC) are described in which a simple separation procedure using ammonium sulfate stabilization of bound steroid and extraction of free steroid into toluene scintillant allows an \"in vial\" assay without mechanical separation of the two phase system. Extraction and thin layer chromatographic methods for purification of aldosterone and DOC are free of solvent and plate blank effects. Normal values are given for unconjugated aldosterone and DOC in urine, for aldosterone and DOC in plasma and for aldosterone 18-glucuronide in urine.", "PMID": 960145}
{"id": "pubmed23n0032_3797", "title": "Behavior of 3alpha- and 7alpha-hydroxysteroid dehydrogenases on chenodeoxycholate substituted Sepharose.", "content": "Chenodeoxycholate (3alpha-, 7alpha-dihydroxy-5beta-cholanoate) was linked to Sepharose 4B by an ethylenediamine bridge. When 3alpha-hydroxysteroid dehydrogenase and 7alpha-hydroxysteroid dehydrogenase preparations were applied to a column of covalently linked chenodeoxycholate, both enzymes were retarded at pH 6.7; the 7alpha-OH oriented enzyme more than the 3alpha-OH enzyme. Approximately forty-fold purification of 7alpha-hydroxysteroid dehydrogenase was achieved in one step. Although no significant purification of 3alpha-hydroxysteroid dehydrogenase occurred, the background value in the fluorometric enzymatic estimation of bile acids by eluted 3alpha-hydroxysteroid dehydrogenase was markedly reduced. Molecular weight estimation by Sephadex G-200 gave the values of 47,000 for 3alpha-hydroxysteroid dehydrogenase and 105,000 for 7alpha-hydroxysteroid dehydrogenase.", "contents": "Behavior of 3alpha- and 7alpha-hydroxysteroid dehydrogenases on chenodeoxycholate substituted Sepharose. Chenodeoxycholate (3alpha-, 7alpha-dihydroxy-5beta-cholanoate) was linked to Sepharose 4B by an ethylenediamine bridge. When 3alpha-hydroxysteroid dehydrogenase and 7alpha-hydroxysteroid dehydrogenase preparations were applied to a column of covalently linked chenodeoxycholate, both enzymes were retarded at pH 6.7; the 7alpha-OH oriented enzyme more than the 3alpha-OH enzyme. Approximately forty-fold purification of 7alpha-hydroxysteroid dehydrogenase was achieved in one step. Although no significant purification of 3alpha-hydroxysteroid dehydrogenase occurred, the background value in the fluorometric enzymatic estimation of bile acids by eluted 3alpha-hydroxysteroid dehydrogenase was markedly reduced. Molecular weight estimation by Sephadex G-200 gave the values of 47,000 for 3alpha-hydroxysteroid dehydrogenase and 105,000 for 7alpha-hydroxysteroid dehydrogenase.", "PMID": 960146}
{"id": "pubmed23n0032_3798", "title": "Effect of endogenous corticosterone on the determination of dexamethasone receptor levels in rat liver cytosol.", "content": "The effect of endogenous corticosterone on the quantitative measurement of dexamethasone receptors in liver cytosols from developing rats has been studied. Liver cytosols from adrenalectomized rats were preincubated with increasing concentrations of nonlabeled corticosterone and the levels of detectable dexamethasone receptors were subsequently determined either directly or after removal of unbound corticosterone. Corticosterone concentrations of 50 nM or lower had no significant effect on the specific binding of labeled dexamethasone. Higher concentrations of corticosterone resulted in under-estimation of dexamethasone receptor levels. The mean levels of endogenous corticosterone in liver cytosols from 19.5- to 21.5- day fetuses, 22-day fetuses, 6-day-old immature rats and adult rats were 27.40, 11.91, 0.81 and 4.05 nM, respectively. It is concluded that variations in the levels of circulating corticosterone in the rat under normal physiological conditions have no significant effect on the quantitative measurement of total (occupied and unoccupied) receptor sites for dexamethasone in liver cytosol. This is supported by the finding that prior treatment of liver cytosols, from rats at different stages of development, with charcoal to remove unbound steroids has no effect on the amount of detectable dexamethasone receptors.", "contents": "Effect of endogenous corticosterone on the determination of dexamethasone receptor levels in rat liver cytosol. The effect of endogenous corticosterone on the quantitative measurement of dexamethasone receptors in liver cytosols from developing rats has been studied. Liver cytosols from adrenalectomized rats were preincubated with increasing concentrations of nonlabeled corticosterone and the levels of detectable dexamethasone receptors were subsequently determined either directly or after removal of unbound corticosterone. Corticosterone concentrations of 50 nM or lower had no significant effect on the specific binding of labeled dexamethasone. Higher concentrations of corticosterone resulted in under-estimation of dexamethasone receptor levels. The mean levels of endogenous corticosterone in liver cytosols from 19.5- to 21.5- day fetuses, 22-day fetuses, 6-day-old immature rats and adult rats were 27.40, 11.91, 0.81 and 4.05 nM, respectively. It is concluded that variations in the levels of circulating corticosterone in the rat under normal physiological conditions have no significant effect on the quantitative measurement of total (occupied and unoccupied) receptor sites for dexamethasone in liver cytosol. This is supported by the finding that prior treatment of liver cytosols, from rats at different stages of development, with charcoal to remove unbound steroids has no effect on the amount of detectable dexamethasone receptors.", "PMID": 960147}
{"id": "pubmed23n0032_3799", "title": "Prostaglandins inhibit testosterone secretion by mouse testes in vitro.", "content": "Production of testosterone (T) by decapsulated mouse tests in vitro was significantly inhibited by adding prostaglandin (PG) A1, PGA2 or PGE1 to the incubation medium. Prostaglandin A1 at a concentration of 10(-6)M inhibited T production in this system both in the presence of moderate amounts of hCG (12.5 or 25.0 mIU/ml), and in the absence of gonadotropins. However, in the presence of very high levels of hCG (125.0 mIU/ml), all PGs tested appeared to have had a slight potentiating effect on T production when added in concentrations ranging from 10(-7) to 10(-5)M, and the inhibition of T accumulation in the medium was consistently observed only when the concentration of PGs was increased to 10(-3)M. These results suggest that a direct effect of PGs on testicular steroidogenesis may account for, or contributes to, the decrease in peripheral T levels observed after administration of PGs in vivo.", "contents": "Prostaglandins inhibit testosterone secretion by mouse testes in vitro. Production of testosterone (T) by decapsulated mouse tests in vitro was significantly inhibited by adding prostaglandin (PG) A1, PGA2 or PGE1 to the incubation medium. Prostaglandin A1 at a concentration of 10(-6)M inhibited T production in this system both in the presence of moderate amounts of hCG (12.5 or 25.0 mIU/ml), and in the absence of gonadotropins. However, in the presence of very high levels of hCG (125.0 mIU/ml), all PGs tested appeared to have had a slight potentiating effect on T production when added in concentrations ranging from 10(-7) to 10(-5)M, and the inhibition of T accumulation in the medium was consistently observed only when the concentration of PGs was increased to 10(-3)M. These results suggest that a direct effect of PGs on testicular steroidogenesis may account for, or contributes to, the decrease in peripheral T levels observed after administration of PGs in vivo.", "PMID": 960148}
{"id": "pubmed23n0032_3800", "title": "A new class of long-acting hormonal steroid preparation: synthesis of dimeric androgenes coupled at C3-C3 and C17-C3 and of an androgen-progestogen combination.", "content": "3beta-Hydroxy-4-androsten-17-one was prepared from 4-androsten-3,17-dione according to the method of Klimstra and Colton (1) and dimerized by means of esterification with succinic acid. The reduction with lithium-tri-t-butoxyaluminium hydride gave a testosterone derivative coupled between C3-C3 which showed after a single injection of 10 mg a protracted but relatively weak androgenic effect in castrated male rats. The direct esterification of testosterone hemisuccinate with 4-androsten-3beta, 17beta-diol gave the testosterone derivative coupled between C17-C3 which showed a more even and more protracted time response curve than testosterone enanthate. The testosterone-ethynodiol succinate also coupled between C17-C3, showed an androgenic depot-effect similar to that of the dimeric C17-C3 testosterone derivative.", "contents": "A new class of long-acting hormonal steroid preparation: synthesis of dimeric androgenes coupled at C3-C3 and C17-C3 and of an androgen-progestogen combination. 3beta-Hydroxy-4-androsten-17-one was prepared from 4-androsten-3,17-dione according to the method of Klimstra and Colton (1) and dimerized by means of esterification with succinic acid. The reduction with lithium-tri-t-butoxyaluminium hydride gave a testosterone derivative coupled between C3-C3 which showed after a single injection of 10 mg a protracted but relatively weak androgenic effect in castrated male rats. The direct esterification of testosterone hemisuccinate with 4-androsten-3beta, 17beta-diol gave the testosterone derivative coupled between C17-C3 which showed a more even and more protracted time response curve than testosterone enanthate. The testosterone-ethynodiol succinate also coupled between C17-C3, showed an androgenic depot-effect similar to that of the dimeric C17-C3 testosterone derivative.", "PMID": 960149}
{"id": "pubmed23n0032_3801", "title": "Hemodynamic derangement for the induction of cerebrovascular fat deposition in normotensive rats on a hypercholesterolemic diet.", "content": "Cerebrovascular ring-like fat deposition, which was noted only in hypertensive rats but never observed in normotensive rats even after they had been fed a high-fat cholesterol (HFC) diet for a long time, was successfully developed in the posterior communicating or other cerebrobasal arteries in normotensive rats fed an HFC diet for ten weeks after bilateral or unilateral carotid artery ligation or basilar artery ligation. These posterior communicating arteries with fat deposits were clearly dilated to a significant extent. These findings corroborated the fact that not only high blood pressure but also hemodynamic derangements induced by hypertension or other causes were important factors for the development of fat deposition in cerebral arteries.", "contents": "Hemodynamic derangement for the induction of cerebrovascular fat deposition in normotensive rats on a hypercholesterolemic diet. Cerebrovascular ring-like fat deposition, which was noted only in hypertensive rats but never observed in normotensive rats even after they had been fed a high-fat cholesterol (HFC) diet for a long time, was successfully developed in the posterior communicating or other cerebrobasal arteries in normotensive rats fed an HFC diet for ten weeks after bilateral or unilateral carotid artery ligation or basilar artery ligation. These posterior communicating arteries with fat deposits were clearly dilated to a significant extent. These findings corroborated the fact that not only high blood pressure but also hemodynamic derangements induced by hypertension or other causes were important factors for the development of fat deposition in cerebral arteries.", "PMID": 960150}
{"id": "pubmed23n0032_3802", "title": "Components of blood pressure and risk of atherothrombotic brain infarction: the Framingham study.", "content": "From a study of the evolution of atherothrombotic brain infarction (ABI) in the Framingham cohort of 5,209 men and women over 18 years of follow-up, it has been ascertained that hypertensionis the most common and most powerful precursor. Atherothrombotic brain infarction developed in hypertensive patients seven times more often than in normotensive patients, and the risk was proportional to the blood pressure throughout its range.", "contents": "Components of blood pressure and risk of atherothrombotic brain infarction: the Framingham study. From a study of the evolution of atherothrombotic brain infarction (ABI) in the Framingham cohort of 5,209 men and women over 18 years of follow-up, it has been ascertained that hypertensionis the most common and most powerful precursor. Atherothrombotic brain infarction developed in hypertensive patients seven times more often than in normotensive patients, and the risk was proportional to the blood pressure throughout its range.", "PMID": 960151}
{"id": "pubmed23n0032_3803", "title": "Intracranial neurosurgical treatment of occlusive cerebrovascular disease.", "content": "Anastomosis of the extracranial superficial temporal artery to the intracranial middle cerebral artery offers an additional source of blood to the cerebral circulation in patients with transient ischemic episodes. Fourteen cases are reported. Two representative cases with three anastomoses demonstrate the use of this technique in occlusion of the internal carotid artery, occlusion of the middle cerebral artery and stenosis of the middle cerebral artery. Indications and contraindications of the procedure are discussed.", "contents": "Intracranial neurosurgical treatment of occlusive cerebrovascular disease. Anastomosis of the extracranial superficial temporal artery to the intracranial middle cerebral artery offers an additional source of blood to the cerebral circulation in patients with transient ischemic episodes. Fourteen cases are reported. Two representative cases with three anastomoses demonstrate the use of this technique in occlusion of the internal carotid artery, occlusion of the middle cerebral artery and stenosis of the middle cerebral artery. Indications and contraindications of the procedure are discussed.", "PMID": 960152}
{"id": "pubmed23n0032_3804", "title": "Noninvasive measurement of velocity profiles and blood flow in the common carotid artery by pulsed Doppler ultrasound.", "content": "A computer-controlled 14-channel pulsed Doppler ultrasound instrument was used as a noninvasive means to evaluate instantaneous velocity profiles and flow in the comlon carotid arteries of 22 healthy persons and in 22 patients. Of the later, 13 had severe obstructions of the extracranial portion of the carotid artery, four had obstruction of the intracranial portion, and five had severe aortic valve insufficiency (AI), with more than 60% regurgitation in all cases. Measurements could be performed within an accuracy of about +/- 20% under clinical conditions and revealed perfusion values of 5 to 8 ml per second (300 to 480 ml per minute) in healthy persons. Of the patients, values less than 3 ml per second (180 ml per minute) were detected in nine, between 3 and 5 mm per second (180 to 300 ml per minute) in four, and normal values in another four, while significant backflow was observed only in patients with severe AI. Consecutive blood flow profiles were recorded every 4 msec; these demonstratedthat, for a period lasting from 40 msec to 280 msec after the initial systolic peak, blood flow decelerated more rapidly in the central portion of the vessel lumen than near the wall. This situation was present in all healthy persons and in most patients with pathological flow. In patients with AI, flow reinversion from reverse to normal began near the vessel wall, while in the middle third of the vessel lumen, blood was still flowing backward. These phenomena seem to be in agreement with the theoretical and experimental findings of Wormersly, M\u00fcller, and others. The AI patients who underwent artificial valve implantation were studied ten days after operation and showed no or little backflow in the common carotid artery at that time.", "contents": "Noninvasive measurement of velocity profiles and blood flow in the common carotid artery by pulsed Doppler ultrasound. A computer-controlled 14-channel pulsed Doppler ultrasound instrument was used as a noninvasive means to evaluate instantaneous velocity profiles and flow in the comlon carotid arteries of 22 healthy persons and in 22 patients. Of the later, 13 had severe obstructions of the extracranial portion of the carotid artery, four had obstruction of the intracranial portion, and five had severe aortic valve insufficiency (AI), with more than 60% regurgitation in all cases. Measurements could be performed within an accuracy of about +/- 20% under clinical conditions and revealed perfusion values of 5 to 8 ml per second (300 to 480 ml per minute) in healthy persons. Of the patients, values less than 3 ml per second (180 ml per minute) were detected in nine, between 3 and 5 mm per second (180 to 300 ml per minute) in four, and normal values in another four, while significant backflow was observed only in patients with severe AI. Consecutive blood flow profiles were recorded every 4 msec; these demonstratedthat, for a period lasting from 40 msec to 280 msec after the initial systolic peak, blood flow decelerated more rapidly in the central portion of the vessel lumen than near the wall. This situation was present in all healthy persons and in most patients with pathological flow. In patients with AI, flow reinversion from reverse to normal began near the vessel wall, while in the middle third of the vessel lumen, blood was still flowing backward. These phenomena seem to be in agreement with the theoretical and experimental findings of Wormersly, M\u00fcller, and others. The AI patients who underwent artificial valve implantation were studied ten days after operation and showed no or little backflow in the common carotid artery at that time.", "PMID": 960155}
{"id": "pubmed23n0032_3805", "title": "The reliability of clinical predictors of extracranial artery disease.", "content": "The records of 628 patients admitted to the Joint Study of Extracranial Arterial Occlusion with transient symptoms of carotid system ischemic disease were examined to determine the accuracy of predicting disease of the extracranial internal carotid artery on the basis of clinical information alone. A patient with a history of episodes of amaurosis fugax is more likely to have a lesion of the internal carotid artery on the same side than if he were having only transient cerebral ischemic attacks. In patients with transient symptoms and a carotid bruit on the appropriate side, the incidence of an angiographically normal carotid artery was 15%. In those patients with transient symptoms and no palpable pulsation in the cervical region on the appropirate side, the incidence of an angiographically normal carotid artery was zero.", "contents": "The reliability of clinical predictors of extracranial artery disease. The records of 628 patients admitted to the Joint Study of Extracranial Arterial Occlusion with transient symptoms of carotid system ischemic disease were examined to determine the accuracy of predicting disease of the extracranial internal carotid artery on the basis of clinical information alone. A patient with a history of episodes of amaurosis fugax is more likely to have a lesion of the internal carotid artery on the same side than if he were having only transient cerebral ischemic attacks. In patients with transient symptoms and a carotid bruit on the appropriate side, the incidence of an angiographically normal carotid artery was 15%. In those patients with transient symptoms and no palpable pulsation in the cervical region on the appropirate side, the incidence of an angiographically normal carotid artery was zero.", "PMID": 960156}
{"id": "pubmed23n0032_3806", "title": "Isolated ophthalmic migraine in the differential diagnosis of cerebro-ocular ischemia.", "content": "Thirty-three of 129 patients who incurred isolated ophthalmic migraine had monocular attacks of scotomatous visual field loss. Fifteen of 33 patients with monocular attacks had immediate or remote evidence of vascular disease. Four patients had carotid bruits on the same side as the monocular attacks and low ophthalmodynamometer readings. One patient had ischemic optic neuropathy and two had atheromatous disease (advanced stage in one patient). Forty-five percent of the patients with monocular attacks and only 13% of the remaining patients with homonymous attacks had vascular complications. This represents an important finding even in such a small group of patients. It is felt that, whether the vascular problems are trigger mechanisms or coexistence pathology to the migraine-type attack, one should strongly suspect such an association when a patient describes a monocular attack and one should look for a possible vascular explanation other than migraine.", "contents": "Isolated ophthalmic migraine in the differential diagnosis of cerebro-ocular ischemia. Thirty-three of 129 patients who incurred isolated ophthalmic migraine had monocular attacks of scotomatous visual field loss. Fifteen of 33 patients with monocular attacks had immediate or remote evidence of vascular disease. Four patients had carotid bruits on the same side as the monocular attacks and low ophthalmodynamometer readings. One patient had ischemic optic neuropathy and two had atheromatous disease (advanced stage in one patient). Forty-five percent of the patients with monocular attacks and only 13% of the remaining patients with homonymous attacks had vascular complications. This represents an important finding even in such a small group of patients. It is felt that, whether the vascular problems are trigger mechanisms or coexistence pathology to the migraine-type attack, one should strongly suspect such an association when a patient describes a monocular attack and one should look for a possible vascular explanation other than migraine.", "PMID": 960157}
{"id": "pubmed23n0032_3807", "title": "Noninavasive angiography for the diagnosis of carotid artery disease using Doppler ultrasound (carotid artery Doppler).", "content": "To detect stenosis in the carotid artery with a bidirectional continuous-wave Doppler ultrasound device, the following noninvasive procedure, applied on 800 patients and compared with 249 angiograms of 186 patients, has proved to be about 90% reliable. Measurements of flow signals were taken over the terminal branches of the ophthalmic artery (supratrochlear and supraorbital arteries) and averaged. Compression of superficial temporal and facial arteries revealed flow direction and common carotid artery compression revealed the supplying blood vessel and the effectiveness of the circle of Willis. Measurements over the common carotid arteries were used to evaluate peripheral resistance. A set of eight criteria was used; the diagnostic value of each criterion was calculated by comparing 138 pathological Doppler findings in 123 patients with the angiograms. If reverse flow direction in supratrochlear or supraorbital artery was used alone, only 43% correct diagnoses would have resulted. A more severe stenosis is not necessarily correlated with a more weighted criterion; a subset of criteria is less efficient than the combination of all criteria. Application during 32 extracranial endarterectomies on 28 patients informed the surgeon immediately about the hemodynamic effect of the surgical intervention. Rethrombosis was diagnosed in two patients by postoperative Doppler examination.", "contents": "Noninavasive angiography for the diagnosis of carotid artery disease using Doppler ultrasound (carotid artery Doppler). To detect stenosis in the carotid artery with a bidirectional continuous-wave Doppler ultrasound device, the following noninvasive procedure, applied on 800 patients and compared with 249 angiograms of 186 patients, has proved to be about 90% reliable. Measurements of flow signals were taken over the terminal branches of the ophthalmic artery (supratrochlear and supraorbital arteries) and averaged. Compression of superficial temporal and facial arteries revealed flow direction and common carotid artery compression revealed the supplying blood vessel and the effectiveness of the circle of Willis. Measurements over the common carotid arteries were used to evaluate peripheral resistance. A set of eight criteria was used; the diagnostic value of each criterion was calculated by comparing 138 pathological Doppler findings in 123 patients with the angiograms. If reverse flow direction in supratrochlear or supraorbital artery was used alone, only 43% correct diagnoses would have resulted. A more severe stenosis is not necessarily correlated with a more weighted criterion; a subset of criteria is less efficient than the combination of all criteria. Application during 32 extracranial endarterectomies on 28 patients informed the surgeon immediately about the hemodynamic effect of the surgical intervention. Rethrombosis was diagnosed in two patients by postoperative Doppler examination.", "PMID": 960153}
{"id": "pubmed23n0032_3808", "title": "Respiratory rate and pattern disturbances in acute brain stem infarction.", "content": "Respiratory rates and patterns were studied in 23 patients with acute brain stem infarction using impedance pneumography. Autopsy was obtained in six of eight fatal cases. Pontine lesions were present in all patients, with coexistent infarction of midbrain in four and of medulla in nine. Respiratory rate and pattern abnormalities observed included Cheyne-Stokes respiration, Cheynb-Stokes variant pattern and tachypnea. Abnormalities of respiratory rate and pattern of varying duration were observed at some time in all patients. All patients in whom prominent Cheyne-Stokes respiration or tachypnea were observed had extensive bilateral pontine lesions involving both basal and tegmental portions. However, not all patients with large pontine infarcts had Cheyne-Stokes respiration or tachypnea. Cheyne-Stokes respiration was prominent in four patients (two fatal, two nonfatal). Cheyne-Stokes variant pattern was present frequently in four patients (one fatal, three nonfatal). Sustained tachypnea developed in five patients, four of whom died. In ten patients (one fatal, nine nonfatal), normal respiratory rate and pattern predominated with only rare or occasional apperance of Cheyne-Stokes respiration or Cheyne-Stokes variant pattern, especially during sleep. The types of respiratory rate and pattern abnormalities in acute brain stem infarction were not specifically related to the level of lesions, but rather to the size and bilaterality of the lesions. Respiratory alkalosis was present in varying degrees in most patients with either tachypnea or prominent CSR.", "contents": "Respiratory rate and pattern disturbances in acute brain stem infarction. Respiratory rates and patterns were studied in 23 patients with acute brain stem infarction using impedance pneumography. Autopsy was obtained in six of eight fatal cases. Pontine lesions were present in all patients, with coexistent infarction of midbrain in four and of medulla in nine. Respiratory rate and pattern abnormalities observed included Cheyne-Stokes respiration, Cheynb-Stokes variant pattern and tachypnea. Abnormalities of respiratory rate and pattern of varying duration were observed at some time in all patients. All patients in whom prominent Cheyne-Stokes respiration or tachypnea were observed had extensive bilateral pontine lesions involving both basal and tegmental portions. However, not all patients with large pontine infarcts had Cheyne-Stokes respiration or tachypnea. Cheyne-Stokes respiration was prominent in four patients (two fatal, two nonfatal). Cheyne-Stokes variant pattern was present frequently in four patients (one fatal, three nonfatal). Sustained tachypnea developed in five patients, four of whom died. In ten patients (one fatal, nine nonfatal), normal respiratory rate and pattern predominated with only rare or occasional apperance of Cheyne-Stokes respiration or Cheyne-Stokes variant pattern, especially during sleep. The types of respiratory rate and pattern abnormalities in acute brain stem infarction were not specifically related to the level of lesions, but rather to the size and bilaterality of the lesions. Respiratory alkalosis was present in varying degrees in most patients with either tachypnea or prominent CSR.", "PMID": 960158}
{"id": "pubmed23n0032_3809", "title": "Fibromuscular dysplasia and multiple dissecting aneurysms of intracranial arteries. A further cause of Moyamoya syndrome.", "content": "A 16-year-old boy, who had sudden left-sided hemiplegia, died two weeks following onset of symptoms. A right carotid angiogram showed stenosis at the termination of the internal carotid artery. The middle cerebral artery had a beaded appearance and some of its branches were occluded. A basal \"moyamoya\" syndrome and transdural anastomoses were present. At autopsy, multiple intracranial dissecting aneurysms were found. Arteries of the body displayed fibromuscular dysplasia (FMD). The relevance of dysplastic changes of intracranial arteries and the relationship to moyamoya syndrome are discussed.", "contents": "Fibromuscular dysplasia and multiple dissecting aneurysms of intracranial arteries. A further cause of Moyamoya syndrome. A 16-year-old boy, who had sudden left-sided hemiplegia, died two weeks following onset of symptoms. A right carotid angiogram showed stenosis at the termination of the internal carotid artery. The middle cerebral artery had a beaded appearance and some of its branches were occluded. A basal \"moyamoya\" syndrome and transdural anastomoses were present. At autopsy, multiple intracranial dissecting aneurysms were found. Arteries of the body displayed fibromuscular dysplasia (FMD). The relevance of dysplastic changes of intracranial arteries and the relationship to moyamoya syndrome are discussed.", "PMID": 960159}
{"id": "pubmed23n0032_3810", "title": "Flow and compartmental weight in relation to the cours of stroke.", "content": "Using a scintillation camera system, hemispheric and regional cerebral blood flow was measured repeatedly during the course after a stroke. In 20 patients who improved clinically mean hemispheric and regional flow and relative weight of rapidly perfused compartment increased, while these values decreased in 15 patients, on an average, whose clinical condition deteriorated or did not change. The changes of flow and relative weight were significantly different between the two groups. Futhermore, the relationship between changes in clinical condition, scored according to a rating scale, and changes in flow and compartmental weight was proved by significant Spearman rank correlation coefficients. In six cases hyperperfused areas in locations of disturbed neurological function were observed; these areas were found to be ischemic at measurements done early and late in the cours after the stroke. This type of hyperperfusion was related to beneficial prognosis. The results indicate a shift of tissue form fast to slowly cleared compartment after a cerebrovascular attack. If tissue morphology is not entirely destroyed, recovery might occur; this results in an increase of recorded weight of rapidly clearing compartments, which correlates to the clinical course.", "contents": "Flow and compartmental weight in relation to the cours of stroke. Using a scintillation camera system, hemispheric and regional cerebral blood flow was measured repeatedly during the course after a stroke. In 20 patients who improved clinically mean hemispheric and regional flow and relative weight of rapidly perfused compartment increased, while these values decreased in 15 patients, on an average, whose clinical condition deteriorated or did not change. The changes of flow and relative weight were significantly different between the two groups. Futhermore, the relationship between changes in clinical condition, scored according to a rating scale, and changes in flow and compartmental weight was proved by significant Spearman rank correlation coefficients. In six cases hyperperfused areas in locations of disturbed neurological function were observed; these areas were found to be ischemic at measurements done early and late in the cours after the stroke. This type of hyperperfusion was related to beneficial prognosis. The results indicate a shift of tissue form fast to slowly cleared compartment after a cerebrovascular attack. If tissue morphology is not entirely destroyed, recovery might occur; this results in an increase of recorded weight of rapidly clearing compartments, which correlates to the clinical course.", "PMID": 960160}
{"id": "pubmed23n0032_3811", "title": "Noninvasive angiography for the diagnosis of vertebral artery disease using Doppler ultrasound (vertebral artery Doppler).", "content": "A transoral noninvasive procedure in the oropharynx using local anesthesia was applied to detect flow in the vertebral arteries with a bidirectional continuous-wave Doppler ultrasound system. Common carotid artery compression was used to identify the vertebral artery. Flow direction, amplitude of flow signals, diastolic flow, and reaction of flow on common carotid artery compression served as diagnostic parameters. The procedure was applied in 90 patients of whom 42 underwent angiography. The method has proved to be 82% accurate. It was most reliable in the diagnosis of occlusion or aplasia, subclavian steal and normalcy, and was less reliable in the detection of stenosis or hypoplasia of a vertebral artery. Eleven patients with subclavin steal, five patients with a missing vertebral artery  three patients with hypoplasia or stenosis, and 15 patients with normal angiorgraphical findings were correctly diagnosed by Doppler; normal Doppler findings were present in three patients with a mission or stenosed vertebral artery. Those patients (five) with Doppler indications of subclavian steal (one patient), missing vertebral artery (two patients), or stenosis (two patients) had normal angiograms. Application of the Doppler procedure, after 11 subclavin endarterectomies, informed the surgeon immediately about the hemodynamic effect of surgical intervention. Rethrombosis was diagnosed in two patients by postoperative Doppler examination.", "contents": "Noninvasive angiography for the diagnosis of vertebral artery disease using Doppler ultrasound (vertebral artery Doppler). A transoral noninvasive procedure in the oropharynx using local anesthesia was applied to detect flow in the vertebral arteries with a bidirectional continuous-wave Doppler ultrasound system. Common carotid artery compression was used to identify the vertebral artery. Flow direction, amplitude of flow signals, diastolic flow, and reaction of flow on common carotid artery compression served as diagnostic parameters. The procedure was applied in 90 patients of whom 42 underwent angiography. The method has proved to be 82% accurate. It was most reliable in the diagnosis of occlusion or aplasia, subclavian steal and normalcy, and was less reliable in the detection of stenosis or hypoplasia of a vertebral artery. Eleven patients with subclavin steal, five patients with a missing vertebral artery  three patients with hypoplasia or stenosis, and 15 patients with normal angiorgraphical findings were correctly diagnosed by Doppler; normal Doppler findings were present in three patients with a mission or stenosed vertebral artery. Those patients (five) with Doppler indications of subclavian steal (one patient), missing vertebral artery (two patients), or stenosis (two patients) had normal angiograms. Application of the Doppler procedure, after 11 subclavin endarterectomies, informed the surgeon immediately about the hemodynamic effect of surgical intervention. Rethrombosis was diagnosed in two patients by postoperative Doppler examination.", "PMID": 960154}
{"id": "pubmed23n0032_3812", "title": "Cerebral atherosclerosis in Uganda.", "content": "A study of arterial circles of Willis from a series of 715 consecutive autopsies in Ugandan Africans shows that severity of cerebral atherosclerosis increases with age. No significant difference exists between men and women nor among main tribal groups. The severity is less in Ugandan Africans than in United States blacks but is approximately the same in United States Caucasians. However, the Ugandan Africans show a more severe cerebral atherosclerotic involvement than do the Nigerians.", "contents": "Cerebral atherosclerosis in Uganda. A study of arterial circles of Willis from a series of 715 consecutive autopsies in Ugandan Africans shows that severity of cerebral atherosclerosis increases with age. No significant difference exists between men and women nor among main tribal groups. The severity is less in Ugandan Africans than in United States blacks but is approximately the same in United States Caucasians. However, the Ugandan Africans show a more severe cerebral atherosclerotic involvement than do the Nigerians.", "PMID": 960161}
{"id": "pubmed23n0032_3813", "title": "Platelet aggregability measured by screen filtration pressure method in cerebrovascular diseases.", "content": "Platelet aggregability was measured using the screen filtration pressure (SFP) method in 50 elderly healthy people, 93 persons with essential hypertension, 166 patients with cerebral thrombosis at the recovery stage (more than two months after onset), and 74 patients with cerebral hemorrhage at the recovery stage. SFP by 3 muM ADP in the healthy persons, the hypertensive patients, and the patients in the recovery stages of hemorrhage and thrombosis were 148.7 +/- 53.5, 176.2 +/- 74.4, 189.8 +/- 58.3 and 206.3 +/- 58.9 mm Hg, respectively. The differences of the SFP between the Healthy and each of the diseased groups were statistically significant (P less than 0.01 to 0.05). Meanwhile, SFP of nine patients with cerebral thrombosis and 18 patients with hemorrhage was measured during their time course of disease from the onset to 180 days. SFP in the acute stage of thrombosis showed an increase and a gradual decrease during the time course, while SFP in the acute stage of hemorrhage showed the opposite -a decrease and a gradual increase. A statistically significant difference was observed between both groups within 30 days from onset (P less than 0.001). Screen filtration pressure in the acute stage of hemorrhage showed 95.2 +/- 17.7 mm Hg in nine survival cases and 194.0 +/- 96.2 mm Hg in nine deaths with ten days from the onset. The difference was statistically significant (P less than 0.001). Such results suggest a role of platelets in cerebral thrombosis and hemorrhage and a usefulness in differential diagnosis of both diseases.", "contents": "Platelet aggregability measured by screen filtration pressure method in cerebrovascular diseases. Platelet aggregability was measured using the screen filtration pressure (SFP) method in 50 elderly healthy people, 93 persons with essential hypertension, 166 patients with cerebral thrombosis at the recovery stage (more than two months after onset), and 74 patients with cerebral hemorrhage at the recovery stage. SFP by 3 muM ADP in the healthy persons, the hypertensive patients, and the patients in the recovery stages of hemorrhage and thrombosis were 148.7 +/- 53.5, 176.2 +/- 74.4, 189.8 +/- 58.3 and 206.3 +/- 58.9 mm Hg, respectively. The differences of the SFP between the Healthy and each of the diseased groups were statistically significant (P less than 0.01 to 0.05). Meanwhile, SFP of nine patients with cerebral thrombosis and 18 patients with hemorrhage was measured during their time course of disease from the onset to 180 days. SFP in the acute stage of thrombosis showed an increase and a gradual decrease during the time course, while SFP in the acute stage of hemorrhage showed the opposite -a decrease and a gradual increase. A statistically significant difference was observed between both groups within 30 days from onset (P less than 0.001). Screen filtration pressure in the acute stage of hemorrhage showed 95.2 +/- 17.7 mm Hg in nine survival cases and 194.0 +/- 96.2 mm Hg in nine deaths with ten days from the onset. The difference was statistically significant (P less than 0.001). Such results suggest a role of platelets in cerebral thrombosis and hemorrhage and a usefulness in differential diagnosis of both diseases.", "PMID": 960162}
{"id": "pubmed23n0032_3814", "title": "Mechanisms of postischemic brain edema: contribution of circulatory factors.", "content": "Controlled cerebral ischemia was produced in rabbits by bilateral occlusion of the common carotid arteries and restriction of collateral blood flow by a decrease of the systemic arterial pressure to a desirable level (by hemorrhage into a pressurized reservior system). The following circulatory parameters were simultaneously monitored: systemic arterial pressure (SAP), pressure in the circle of Willis (Pcw), systemic venous pressure (SVP), and pressure in the sagittal venous sinus of brain (Pvs). The cerebral blood flow (CBF) was measured by means of the H2-clearance method, and the brain volume (BrV) changes were evaluated with a mechanical system of the sterotaxic device. It has been concluded that the pre-edematous changes in the brain tissue arise during deep ischemis but an important factor in the brain edema development is the recovery of the CBF with and increase of the intravascular pressure closely related to the brain blood volume augmentation. The latter may be pronouced because of diminution of the blood outflow from the brain when the SVP is increased. The compensation for the BrV increase (caused either by brain blood volume augmentation or by brain edema) is obtained by Pcw decrease probably due to resistance rise in the internal carotid and vertebral arteries. The brain edema may be additionally compensated by an active decrease of the systemic arterial pressure.", "contents": "Mechanisms of postischemic brain edema: contribution of circulatory factors. Controlled cerebral ischemia was produced in rabbits by bilateral occlusion of the common carotid arteries and restriction of collateral blood flow by a decrease of the systemic arterial pressure to a desirable level (by hemorrhage into a pressurized reservior system). The following circulatory parameters were simultaneously monitored: systemic arterial pressure (SAP), pressure in the circle of Willis (Pcw), systemic venous pressure (SVP), and pressure in the sagittal venous sinus of brain (Pvs). The cerebral blood flow (CBF) was measured by means of the H2-clearance method, and the brain volume (BrV) changes were evaluated with a mechanical system of the sterotaxic device. It has been concluded that the pre-edematous changes in the brain tissue arise during deep ischemis but an important factor in the brain edema development is the recovery of the CBF with and increase of the intravascular pressure closely related to the brain blood volume augmentation. The latter may be pronouced because of diminution of the blood outflow from the brain when the SVP is increased. The compensation for the BrV increase (caused either by brain blood volume augmentation or by brain edema) is obtained by Pcw decrease probably due to resistance rise in the internal carotid and vertebral arteries. The brain edema may be additionally compensated by an active decrease of the systemic arterial pressure.", "PMID": 960163}
{"id": "pubmed23n0032_3815", "title": "Doppler sonography diagnosis of cerebrovascular disease.", "content": "Sonographic patterns for the Doppler ophthalmic test (DOT) were determined by reviewing the type of graphic record obtained from 25 patients who had undergone angiography. Three principal patterns were identified, depending upon whether there was augmentation (Type 1), diminution (Type 2) or no change (Type 3) in the character of the supraorbital Doppler signal upon temporal artery compression. Type 1 records were seen in 28 of 33 instances where the carotid arteries remained patent. Type 2 records occurred in 10 of 14 instances where there was significant carotid stenoses or tortuosity and in two instances in a patient with a subclavian steal syndrome. A \"false-positive\" test was recorded in one instance in a patient with vascular headaches. There were three Type 3 records.", "contents": "Doppler sonography diagnosis of cerebrovascular disease. Sonographic patterns for the Doppler ophthalmic test (DOT) were determined by reviewing the type of graphic record obtained from 25 patients who had undergone angiography. Three principal patterns were identified, depending upon whether there was augmentation (Type 1), diminution (Type 2) or no change (Type 3) in the character of the supraorbital Doppler signal upon temporal artery compression. Type 1 records were seen in 28 of 33 instances where the carotid arteries remained patent. Type 2 records occurred in 10 of 14 instances where there was significant carotid stenoses or tortuosity and in two instances in a patient with a subclavian steal syndrome. A \"false-positive\" test was recorded in one instance in a patient with vascular headaches. There were three Type 3 records.", "PMID": 960165}
{"id": "pubmed23n0032_3816", "title": "An evaluation of hypocarbia and hypercarbia during carotid endarterectomy.", "content": "One hundred consecutive patients were randomly given hypocarbic (PaCO2 less than 25 torr) or hypercarbic (PaCO2 greater than 60 torr) general anesthesia during carotid endarterectomy to test the effect of the two regimens upon the incidence of postoperative neurological deficit. An indwelling shunt was not used. One patient died, two have permanent neurological deficits and two have temporary neurological deficits. Although hypocarbic patients had fewer neurological complications than hypercarbic patients, the difference was not statistically significant (p less than 0.13). Hypercarbia significantly increased the incidence of intraoperative arrhythmia. Also, no relationship was found between the incidence of postoperative stroke and the internal carotid back pressure or the time of carotid occlusion.", "contents": "An evaluation of hypocarbia and hypercarbia during carotid endarterectomy. One hundred consecutive patients were randomly given hypocarbic (PaCO2 less than 25 torr) or hypercarbic (PaCO2 greater than 60 torr) general anesthesia during carotid endarterectomy to test the effect of the two regimens upon the incidence of postoperative neurological deficit. An indwelling shunt was not used. One patient died, two have permanent neurological deficits and two have temporary neurological deficits. Although hypocarbic patients had fewer neurological complications than hypercarbic patients, the difference was not statistically significant (p less than 0.13). Hypercarbia significantly increased the incidence of intraoperative arrhythmia. Also, no relationship was found between the incidence of postoperative stroke and the internal carotid back pressure or the time of carotid occlusion.", "PMID": 960166}
{"id": "pubmed23n0032_3817", "title": "Carotid blood velocity during cough studies in man.", "content": "Utilizing a Doppler ultrasonic flowmeter catheter, right carotid artery blood velocity was measured during 91 coughing episodes in 16 patients. Such coughing reduced carotid blood velocity by 40 +/- 22% (control = 34 +/- 8 cm per second, cough = 20 +/- 9 cm per second, p less than 0.001). There was an insignificant low degree of corrleation between the level of simultaneously recorded mean right pressure and the percent decline of peak carotid blood velocity, suggesting that impaired venous return was not the only factor responsible for the observed changes. It is concluded that (1)coughing diminishes phasic carotid blood velocity and (2)reduced cerebral perfusion may play a role in the pathogenesis of cough syncope.", "contents": "Carotid blood velocity during cough studies in man. Utilizing a Doppler ultrasonic flowmeter catheter, right carotid artery blood velocity was measured during 91 coughing episodes in 16 patients. Such coughing reduced carotid blood velocity by 40 +/- 22% (control = 34 +/- 8 cm per second, cough = 20 +/- 9 cm per second, p less than 0.001). There was an insignificant low degree of corrleation between the level of simultaneously recorded mean right pressure and the percent decline of peak carotid blood velocity, suggesting that impaired venous return was not the only factor responsible for the observed changes. It is concluded that (1)coughing diminishes phasic carotid blood velocity and (2)reduced cerebral perfusion may play a role in the pathogenesis of cough syncope.", "PMID": 960164}
{"id": "pubmed23n0032_3818", "title": "Acute gastric changes in patients with acute stroke. Part 2: gastroendoscopic findings and biochemical observation of urinary noradrenalin, adrenalin, 17-OHCS and serum gastrin.", "content": "To investigate the relationships between gastric change accompanying patients with acute stroke and the function of the autonomic nervous system and pituitary-adrenal system, studies on the gastroendoscopic technique and biochemical observations were done in 122 patients. The mean urinary noradrenalin and adrenalin excretions were elevated in patients with acute gastric changes, especially in patients with multiple erosions and acute ulcers. An elevation of the mean fasting serum gastrin concentration was found in patients with acute ulcers and no acute gastric changes. The mean urinary 17-OHCS excretion was increased in patients with multiple erosions, petechiae and acute ulcers. In conclusion, the sympathetic and parasympathetic nervous system and the pituitary-adrenal system have respectively different roles in the production of various types of acute gastric changes in patients.", "contents": "Acute gastric changes in patients with acute stroke. Part 2: gastroendoscopic findings and biochemical observation of urinary noradrenalin, adrenalin, 17-OHCS and serum gastrin. To investigate the relationships between gastric change accompanying patients with acute stroke and the function of the autonomic nervous system and pituitary-adrenal system, studies on the gastroendoscopic technique and biochemical observations were done in 122 patients. The mean urinary noradrenalin and adrenalin excretions were elevated in patients with acute gastric changes, especially in patients with multiple erosions and acute ulcers. An elevation of the mean fasting serum gastrin concentration was found in patients with acute ulcers and no acute gastric changes. The mean urinary 17-OHCS excretion was increased in patients with multiple erosions, petechiae and acute ulcers. In conclusion, the sympathetic and parasympathetic nervous system and the pituitary-adrenal system have respectively different roles in the production of various types of acute gastric changes in patients.", "PMID": 960167}
{"id": "pubmed23n0032_3819", "title": "Pharmacological control of local oxygen regulation mechanisms in brain tissue.", "content": "The effect of several agents active on autonomic nervous system functions was tested on brain oxygen autoregulation parameters. It was found that atropine, propranolol and isoproterenol had no influence in abolishing the measured parameters. Phenoxybenzamine, tolazoline and dibenamine all suppress autoregulation. In an additional experimental series, a phenoxybenzamine infusion was given during O2 breathing. The infusion induced, in most cases, an additional rise in TpO2 (tissue pressure of oxygen, which refers to the partial pressure [in mm Hg] of this gas at the measuring tip of the electrode). It is concluded that an alpha-adrenergic mechanism is part of the autoregulation process. Also, the increase in brain TpO2 induced by 59% O2-5% Co2 breathing seems to be blocked or reversed by alpha-adrenolytic drugs, thus supporting the thinking that the effect of CO2 on cerebral blood flow is at least in part mediated through an alpha-adrenergic response.", "contents": "Pharmacological control of local oxygen regulation mechanisms in brain tissue. The effect of several agents active on autonomic nervous system functions was tested on brain oxygen autoregulation parameters. It was found that atropine, propranolol and isoproterenol had no influence in abolishing the measured parameters. Phenoxybenzamine, tolazoline and dibenamine all suppress autoregulation. In an additional experimental series, a phenoxybenzamine infusion was given during O2 breathing. The infusion induced, in most cases, an additional rise in TpO2 (tissue pressure of oxygen, which refers to the partial pressure [in mm Hg] of this gas at the measuring tip of the electrode). It is concluded that an alpha-adrenergic mechanism is part of the autoregulation process. Also, the increase in brain TpO2 induced by 59% O2-5% Co2 breathing seems to be blocked or reversed by alpha-adrenolytic drugs, thus supporting the thinking that the effect of CO2 on cerebral blood flow is at least in part mediated through an alpha-adrenergic response.", "PMID": 960168}
{"id": "pubmed23n0032_3820", "title": "Prognosis of occlusive cerbrovascular diseases in normotensive and hypertensive subjects.", "content": "Comparison of the clinical features, especially prognosis, in cerebral infarction was made between nine normotensive subjects and 16 hypertensive patients with an 80% stenosis or occlusion of the intracranial or extracranial arteries. Our own criteria for evaluating hypertension were employed on the basis of the following items: a past history of hypertension, blood pressure levels on admission and during hospitalization, degree of retinopathy, and ECG changes. In 17 of 25 cases, brain circulation was measured by the intravenous RISA technique. Abnormalities of the EEG and reduction of cranial blood flow were greater, and an early prognosis for neurological deficits in the first two months after the onset of stroke was poorer in the hypertensive group than inthe normotensive group. These results are contradictory to the observations of others.", "contents": "Prognosis of occlusive cerbrovascular diseases in normotensive and hypertensive subjects. Comparison of the clinical features, especially prognosis, in cerebral infarction was made between nine normotensive subjects and 16 hypertensive patients with an 80% stenosis or occlusion of the intracranial or extracranial arteries. Our own criteria for evaluating hypertension were employed on the basis of the following items: a past history of hypertension, blood pressure levels on admission and during hospitalization, degree of retinopathy, and ECG changes. In 17 of 25 cases, brain circulation was measured by the intravenous RISA technique. Abnormalities of the EEG and reduction of cranial blood flow were greater, and an early prognosis for neurological deficits in the first two months after the onset of stroke was poorer in the hypertensive group than inthe normotensive group. These results are contradictory to the observations of others.", "PMID": 960169}
{"id": "pubmed23n0032_3821", "title": "Vascular clips for the microsurgical treatment of stroke.", "content": "A synopsis of the types of vascular clips (malleable clips, screw clips, and spring clips) used by neurosurgeons during this century is presented. A review of the pertinent literature is included.", "contents": "Vascular clips for the microsurgical treatment of stroke. A synopsis of the types of vascular clips (malleable clips, screw clips, and spring clips) used by neurosurgeons during this century is presented. A review of the pertinent literature is included.", "PMID": 960173}
{"id": "pubmed23n0032_3822", "title": "Prognosis of patients with middle cerebral artery occlusion.", "content": "The long-term prognosis of 78 stroke patients with occlusion of the middle cerebral artery (MCA) or its branches is described. The mean age of the patients was 44 years. The mortality rate in the acute phase was 5%. The acute and total mortality rates of men were higher than those of women (p less than 0.05). Life-table analysis gave 94% probability for one year's survival, 84% for three years' survival, and 78% for five years' survival. Subsequent strokes were twice as common as cardiovascular events as the cause of death. Seventy-two percent of the survivors became fully independent in activities of daily living (ADL) , 27% required assistance, 1% was totally disabled, and 43% returned to work. Left-sided occlusion was overrepresented in those who died (p less than 0.00(1) and those who returned to work (p less than 0.05), and right-sided occlusion was overrepresented in those who required assistance in ADL (p less than 0.05).", "contents": "Prognosis of patients with middle cerebral artery occlusion. The long-term prognosis of 78 stroke patients with occlusion of the middle cerebral artery (MCA) or its branches is described. The mean age of the patients was 44 years. The mortality rate in the acute phase was 5%. The acute and total mortality rates of men were higher than those of women (p less than 0.05). Life-table analysis gave 94% probability for one year's survival, 84% for three years' survival, and 78% for five years' survival. Subsequent strokes were twice as common as cardiovascular events as the cause of death. Seventy-two percent of the survivors became fully independent in activities of daily living (ADL) , 27% required assistance, 1% was totally disabled, and 43% returned to work. Left-sided occlusion was overrepresented in those who died (p less than 0.00(1) and those who returned to work (p less than 0.05), and right-sided occlusion was overrepresented in those who required assistance in ADL (p less than 0.05).", "PMID": 960172}
{"id": "pubmed23n0032_3823", "title": "Pyramidal tract responses (PTR) during hypoxia and hypotension.", "content": "In rats with unilateral carotid artery ligation pyramidal tract responses were studied during hypoxia and during trimethaphan-induced hypotension. Observations on EEG activity during hypoxia suggest that unilateral carotid artery ligation produces a more severe perfusion defect in lateral portions of the hemisphere. During hypoxia and during trimethaphan-induced hypotension indirect PTRs disappeared first from the hemisphere on the side of carotid artery ligation and next from the opposite hemisphere. This was followed by loss of direct PTRs in the same order. Animals could not be resuscitated once the direct PTR from the non-ligated hemisphere had disappeared. Hypotension appears to be a late contributing factor in impairing electrocerebral activity during hypoxia in this study.", "contents": "Pyramidal tract responses (PTR) during hypoxia and hypotension. In rats with unilateral carotid artery ligation pyramidal tract responses were studied during hypoxia and during trimethaphan-induced hypotension. Observations on EEG activity during hypoxia suggest that unilateral carotid artery ligation produces a more severe perfusion defect in lateral portions of the hemisphere. During hypoxia and during trimethaphan-induced hypotension indirect PTRs disappeared first from the hemisphere on the side of carotid artery ligation and next from the opposite hemisphere. This was followed by loss of direct PTRs in the same order. Animals could not be resuscitated once the direct PTR from the non-ligated hemisphere had disappeared. Hypotension appears to be a late contributing factor in impairing electrocerebral activity during hypoxia in this study.", "PMID": 960174}
{"id": "pubmed23n0032_3824", "title": "Abortion research in Latin America.", "content": "Surveys dealing with abortion in Latin America have provided useful information despite problems in the collection and use of the data. Considerations that should be taken into account in designing abortion surveys and using the resultant information have been discussed here. Special attention has been paid to the need for a broad definition of \"abortion\" in order to overcome difficulties in gathering information about abortion in Latin America. Surveys have shown increasing incidence of abortion throughout Latin America in the recent past. In examining changes over time it is crucial to interpret clearly and carefully the summary measures of proportion of pregnancies ending in abortion and abortion rates per 1,000 women. It is also important to realize that the level and direction of change of the abortion rate depends on both the rate at which women are becoming pregnant and the proportion of pregnancies ending in abortion. Better survey design and techniques and more careful use of the resulting information will aid in the planning and evaluation of programs aimed at reducing abortion in Latin America.", "contents": "Abortion research in Latin America. Surveys dealing with abortion in Latin America have provided useful information despite problems in the collection and use of the data. Considerations that should be taken into account in designing abortion surveys and using the resultant information have been discussed here. Special attention has been paid to the need for a broad definition of \"abortion\" in order to overcome difficulties in gathering information about abortion in Latin America. Surveys have shown increasing incidence of abortion throughout Latin America in the recent past. In examining changes over time it is crucial to interpret clearly and carefully the summary measures of proportion of pregnancies ending in abortion and abortion rates per 1,000 women. It is also important to realize that the level and direction of change of the abortion rate depends on both the rate at which women are becoming pregnant and the proportion of pregnancies ending in abortion. Better survey design and techniques and more careful use of the resulting information will aid in the planning and evaluation of programs aimed at reducing abortion in Latin America.", "PMID": 960180}
{"id": "pubmed23n0032_3825", "title": "The impact of liberalized abortion legislation on contraceptive practice in Denmark.", "content": "During the fifteen months following the 1973 Danish abortion legislation, the mean annual rate of induced abortion was 23 per 1,000 women aged 15-44 compared with a mean annual rate of 14.5 during the 15 preceding months. Though use of legal abortion has increased, the rate is not of a magnitude to suggest large-scale abandonment of contraception. On the contrary, preconceptive fertility control continues to be widely practiced.", "contents": "The impact of liberalized abortion legislation on contraceptive practice in Denmark. During the fifteen months following the 1973 Danish abortion legislation, the mean annual rate of induced abortion was 23 per 1,000 women aged 15-44 compared with a mean annual rate of 14.5 during the 15 preceding months. Though use of legal abortion has increased, the rate is not of a magnitude to suggest large-scale abandonment of contraception. On the contrary, preconceptive fertility control continues to be widely practiced.", "PMID": 960181}
{"id": "pubmed23n0032_3826", "title": "Coincidental multiple asymptomatic intracranial aneurysms and symptomatic carotid stenosis.", "content": "A patient experiencing transient cerebral ischemic attacks (TIAs) was studied angiographically and found to have a symptomatic stenosis of the appropriate internal carotid artery (ICA) and three asymptomatic intracranial aneurysms. A therapeutic approach to this type of problem is discussed in this article.", "contents": "Coincidental multiple asymptomatic intracranial aneurysms and symptomatic carotid stenosis. A patient experiencing transient cerebral ischemic attacks (TIAs) was studied angiographically and found to have a symptomatic stenosis of the appropriate internal carotid artery (ICA) and three asymptomatic intracranial aneurysms. A therapeutic approach to this type of problem is discussed in this article.", "PMID": 960175}
{"id": "pubmed23n0032_3827", "title": "Scanning electron microscopic appraisal of a new micro T-tube.", "content": "A new micro T-tube, 0.6 mm in outer diameter, has been constructed. Use of this tube has allowed 1-mm common carotid artery (CCA) bypass ofr periods of time up to 22 minutes. After bypass, scanning electron microscopic observation of sutured CCA endothelium showed widespread destruction, scattered attached platelets and other formed blood elements, as well as microthrombi both at suture lines and in areas in contact with T-tubes. Despite microthrombi, 41 of 42 CCAs were patent after anastomosis. Silicone cuffs and 8-0 ties secured T-tubes inside vessels with no apparent difference in underlying endothelial destruction.", "contents": "Scanning electron microscopic appraisal of a new micro T-tube. A new micro T-tube, 0.6 mm in outer diameter, has been constructed. Use of this tube has allowed 1-mm common carotid artery (CCA) bypass ofr periods of time up to 22 minutes. After bypass, scanning electron microscopic observation of sutured CCA endothelium showed widespread destruction, scattered attached platelets and other formed blood elements, as well as microthrombi both at suture lines and in areas in contact with T-tubes. Despite microthrombi, 41 of 42 CCAs were patent after anastomosis. Silicone cuffs and 8-0 ties secured T-tubes inside vessels with no apparent difference in underlying endothelial destruction.", "PMID": 960170}
{"id": "pubmed23n0032_3828", "title": "Cerebrovascular CO2 reactivity in normotensive and hypertensive man.", "content": "Cerebrovascular reactivity to CO2 inhalation and voluntary hyperventilation was studied in seven normotensive subjects and nine hypertensive patients without clinical or angiographical signs of arteriosclerosis. Cerebral blood flow (CBF) was measured by the intracarotid 133Xe clearance method and calculated as the initial slope index. Three to five CBF measurements were made in each patient in the PaCO2 range of 20 to 55 mm Hg. No difference was observed in reactivity between hypertensive and normotensive patients, either during CO2 inhalation or during hyperventilation. The shape of the CBF:PaCO2 curve suggested a decrease in reactivity below a PaCO2 of 30 to 35 mm Hg in both groups. Above a PaCO2 of 35 mm Hg, exponential regression analysis yielded a mean reactivity of 6 +/- 2%, whereas below a PaCO2 of 30 mm Hg it was about 2%. The rise in CBF during CO2 inhalation was not influenced by the intravenous infusion of a small dose of trimethaphan which blocked the concomitant rise in blood pressure.", "contents": "Cerebrovascular CO2 reactivity in normotensive and hypertensive man. Cerebrovascular reactivity to CO2 inhalation and voluntary hyperventilation was studied in seven normotensive subjects and nine hypertensive patients without clinical or angiographical signs of arteriosclerosis. Cerebral blood flow (CBF) was measured by the intracarotid 133Xe clearance method and calculated as the initial slope index. Three to five CBF measurements were made in each patient in the PaCO2 range of 20 to 55 mm Hg. No difference was observed in reactivity between hypertensive and normotensive patients, either during CO2 inhalation or during hyperventilation. The shape of the CBF:PaCO2 curve suggested a decrease in reactivity below a PaCO2 of 30 to 35 mm Hg in both groups. Above a PaCO2 of 35 mm Hg, exponential regression analysis yielded a mean reactivity of 6 +/- 2%, whereas below a PaCO2 of 30 mm Hg it was about 2%. The rise in CBF during CO2 inhalation was not influenced by the intravenous infusion of a small dose of trimethaphan which blocked the concomitant rise in blood pressure.", "PMID": 960176}
{"id": "pubmed23n0032_3829", "title": "The influence of induced abortion on Taiwanese fertility.", "content": "Data on the outcomes of more than 15,000 pregnancies originating between May 1966 and February 1969 were analyzed. The accuracy of the data was evaluated, rates of induced abortion and stillbirth were reported, and the demographic effect of induced abortion was estimated. The demographic effect was defined as the percentage increase in fertility that would have occurred in the absence of induced abortion, assuming no compensating change in alternative fertility control practices. Our principal findings were as follows: 1. We were unable to determine the completeness with which induced abortion was reported in the Registration Study. During the three years covered by the study, rates of induced abortion increased by 54 percent, reflecting a trend in the incidence or in the reporting of events or in both. We concluded that, in any case, the data for the final year of the study, 1968, were more complete than for the earlier two years. 2. Age-specific rates of induced abortion for 1968 displayed a strong urban-rural gradient, being much higher in the city areas than in the rural areas. Within each urban--rural stratum, the rates increases monotonically with age and reached maximum values in the age group 40 and older (553, 436, and 374 per 1,000 pregnancies for city, urban, and rural areas, respectively). 3. Estimates of the demographic effect indicated that, in the absence of induced abortion, the TFR for all Taiwan would have been 12 percent higher in 1968. Under the same assumption, it was estimated that the TFR would have been higher by 16 percent in city areas, 11 percent in urban areas, and 9 percent in rural areas. This urbanization gradient implies that induced abortion contributed to urban-rural fertility differentials. We estimated that about one-third of those differentials were due to induced abortion. 4. Estimates of the demographic effect were also made after adjusting the rates of induced abortion for an assumed level of underreporting of 50 percent. The adjusted estimate of the demographic effect for all Taiwan in 1968 was 19 percent.", "contents": "The influence of induced abortion on Taiwanese fertility. Data on the outcomes of more than 15,000 pregnancies originating between May 1966 and February 1969 were analyzed. The accuracy of the data was evaluated, rates of induced abortion and stillbirth were reported, and the demographic effect of induced abortion was estimated. The demographic effect was defined as the percentage increase in fertility that would have occurred in the absence of induced abortion, assuming no compensating change in alternative fertility control practices. Our principal findings were as follows: 1. We were unable to determine the completeness with which induced abortion was reported in the Registration Study. During the three years covered by the study, rates of induced abortion increased by 54 percent, reflecting a trend in the incidence or in the reporting of events or in both. We concluded that, in any case, the data for the final year of the study, 1968, were more complete than for the earlier two years. 2. Age-specific rates of induced abortion for 1968 displayed a strong urban-rural gradient, being much higher in the city areas than in the rural areas. Within each urban--rural stratum, the rates increases monotonically with age and reached maximum values in the age group 40 and older (553, 436, and 374 per 1,000 pregnancies for city, urban, and rural areas, respectively). 3. Estimates of the demographic effect indicated that, in the absence of induced abortion, the TFR for all Taiwan would have been 12 percent higher in 1968. Under the same assumption, it was estimated that the TFR would have been higher by 16 percent in city areas, 11 percent in urban areas, and 9 percent in rural areas. This urbanization gradient implies that induced abortion contributed to urban-rural fertility differentials. We estimated that about one-third of those differentials were due to induced abortion. 4. Estimates of the demographic effect were also made after adjusting the rates of induced abortion for an assumed level of underreporting of 50 percent. The adjusted estimate of the demographic effect for all Taiwan in 1968 was 19 percent.", "PMID": 960183}
{"id": "pubmed23n0032_3830", "title": "Prognosis of patients with unilateral extracranial occlusion of the internal carotid artery.", "content": "One hundred fifty-five patients (121 men and 34 women) had an angiographically verified unilateral internal carotid artery (ICA) occlusion immediately after carotid bifurcation. The median follow-up period was 53 months. Forty-five percent of the patients were fully independent in the activities of daily living (ADL), 22% required assistance in ADL, 11% were disabled, and 22% died within the follow-up period. The mortality rate was exceptionally low compared with previous series. A life-table analysis of the material gave a 94% probability of surviving the first year, 84% the third year, and 78% the fifth year. Young age was favorable in regard to functional recovery, but it had no influence on mortality. The condition at the acute stage was the most important clinical sign in predicting the prognosis. The prognosis of mortality and functional recovery was poorer for smokers than for nonsmokers, and hypertension had no effect on prognosis. Cerebrovascular disease and coronary heart disease were found to be the cause of death with equal frequency in this study.", "contents": "Prognosis of patients with unilateral extracranial occlusion of the internal carotid artery. One hundred fifty-five patients (121 men and 34 women) had an angiographically verified unilateral internal carotid artery (ICA) occlusion immediately after carotid bifurcation. The median follow-up period was 53 months. Forty-five percent of the patients were fully independent in the activities of daily living (ADL), 22% required assistance in ADL, 11% were disabled, and 22% died within the follow-up period. The mortality rate was exceptionally low compared with previous series. A life-table analysis of the material gave a 94% probability of surviving the first year, 84% the third year, and 78% the fifth year. Young age was favorable in regard to functional recovery, but it had no influence on mortality. The condition at the acute stage was the most important clinical sign in predicting the prognosis. The prognosis of mortality and functional recovery was poorer for smokers than for nonsmokers, and hypertension had no effect on prognosis. Cerebrovascular disease and coronary heart disease were found to be the cause of death with equal frequency in this study.", "PMID": 960171}
{"id": "pubmed23n0032_3831", "title": "Intracranial fibromuscular dysplasia: report of two cases, one with autopsy verification.", "content": "We report two patients with fibromuscular dysplasia (FMD) involving intracranial arteries. In the first patient the diagnosis was made at autopsy. Both patients were symptomatic from this disease and, in addition, displayed unusual hormonal abnormalities which may well have contributed to the genesis or evolution of FMD.", "contents": "Intracranial fibromuscular dysplasia: report of two cases, one with autopsy verification. We report two patients with fibromuscular dysplasia (FMD) involving intracranial arteries. In the first patient the diagnosis was made at autopsy. Both patients were symptomatic from this disease and, in addition, displayed unusual hormonal abnormalities which may well have contributed to the genesis or evolution of FMD.", "PMID": 960177}
{"id": "pubmed23n0032_3832", "title": "Aphasia outcome in stroke: a clinical neuroradiological correlation.", "content": "Fourteen aphasic patients with acute onset of thromboembolic cerebrovascular insults demonstrable by angiography or radioscintigrams who were available for long-term follow-up have been studied. Their aphasia evolution was compared with acute angiographical and radioisotopic findings, and the lesions shown by follow-up computerized axial tomography (CT). Angiographical site of occlusion, evidence of early reopening of occluded vessels, and radioisotopic flow asymmetries including the \"hot-stroke\" luxury perfusion failed to correlate with aphasia outcome. Radioisotopic static images were more helpful by depicting lesion location and number but lacked the definition seen on the CT scan. The long-term CT scan by showing the size, location and number of lesions had a good correlation with aphasia outcome. Those patients with large dominant hemisphere involvements, either one large or many smaller lesions, fared poorly while those with lesser lesions did better. Bilateral lesions, at times evasive clinically, helped to account for significant aphasia residuals.", "contents": "Aphasia outcome in stroke: a clinical neuroradiological correlation. Fourteen aphasic patients with acute onset of thromboembolic cerebrovascular insults demonstrable by angiography or radioscintigrams who were available for long-term follow-up have been studied. Their aphasia evolution was compared with acute angiographical and radioisotopic findings, and the lesions shown by follow-up computerized axial tomography (CT). Angiographical site of occlusion, evidence of early reopening of occluded vessels, and radioisotopic flow asymmetries including the \"hot-stroke\" luxury perfusion failed to correlate with aphasia outcome. Radioisotopic static images were more helpful by depicting lesion location and number but lacked the definition seen on the CT scan. The long-term CT scan by showing the size, location and number of lesions had a good correlation with aphasia outcome. Those patients with large dominant hemisphere involvements, either one large or many smaller lesions, fared poorly while those with lesser lesions did better. Bilateral lesions, at times evasive clinically, helped to account for significant aphasia residuals.", "PMID": 960178}
{"id": "pubmed23n0032_3833", "title": "The prevalence of diabetes mellitus in three population groups in the Republic of Mali.", "content": "Three different adult population groups in Mali were screened for diabetes mellitus by means of a post-prandial blood glucose determination. The prevalence of diabetes for the entire population sampled was found to be 1-4%. Diabetes mellitus was strongly suspected in an additional 8-4%. The prevalence of elevated post-prandial blood glucose levels was found to be the lowest (5-1%) among a group of Bambara farmers who live an active rural life and whose total daily caloric intake is less than 1,000 on the average, only half of it provided by carbohydrates.", "contents": "The prevalence of diabetes mellitus in three population groups in the Republic of Mali. Three different adult population groups in Mali were screened for diabetes mellitus by means of a post-prandial blood glucose determination. The prevalence of diabetes for the entire population sampled was found to be 1-4%. Diabetes mellitus was strongly suspected in an additional 8-4%. The prevalence of elevated post-prandial blood glucose levels was found to be the lowest (5-1%) among a group of Bambara farmers who live an active rural life and whose total daily caloric intake is less than 1,000 on the average, only half of it provided by carbohydrates.", "PMID": 960199}
{"id": "pubmed23n0032_3834", "title": "A tuberculosis treatment survey in Singapore: results at five-years.", "content": "A National Tuberculosis Treatment Survey was instituted in the Republic of Singapore in 1969 with the aim of evaluating the Tuberculosis Treatment Services in Singapore. This five-year follow-up study of the 1969 survey was based on the assessment of the first 1000 consecutive patients who were registered for treatment during the year. Two hundred and forty-six cases were excluded for various reasons, leaving 754 cases for analysis. At the end of 5 years, only 2 (0.3%) were culture positive, 677 (89.8%) were culture negative, 48 cases (6.4%) had died from tuberculosis and 27 cases (3.6%) were lost. The most notable factor which influenced bacteriological positivity was lack of co-operation leading to interruption of chemotherapy. It is concluded that with an efficient organisation highly satisfactory results can be achieved by routine treatment services and prolonged observation of adequately treated patient is not necessary.", "contents": "A tuberculosis treatment survey in Singapore: results at five-years. A National Tuberculosis Treatment Survey was instituted in the Republic of Singapore in 1969 with the aim of evaluating the Tuberculosis Treatment Services in Singapore. This five-year follow-up study of the 1969 survey was based on the assessment of the first 1000 consecutive patients who were registered for treatment during the year. Two hundred and forty-six cases were excluded for various reasons, leaving 754 cases for analysis. At the end of 5 years, only 2 (0.3%) were culture positive, 677 (89.8%) were culture negative, 48 cases (6.4%) had died from tuberculosis and 27 cases (3.6%) were lost. The most notable factor which influenced bacteriological positivity was lack of co-operation leading to interruption of chemotherapy. It is concluded that with an efficient organisation highly satisfactory results can be achieved by routine treatment services and prolonged observation of adequately treated patient is not necessary.", "PMID": 960225}
{"id": "pubmed23n0032_3835", "title": "Biochemical background of some enzymatic tests used for the differentiation of mycobacteria.", "content": "The break-down of benzamide, acetamide, malonamide and allantoin in M. smegmatis was investigated. It has been stated that the uptake of liberated NH3 into the cells, favoured by the presence of an organic acid, occasionally results in a negative NH3 determination. This difficulty can be overcome by an increase of the substrate concentration from 0.8 up to 4 mM. All antoinase activity in mycobacteria can be demonstrated only by an NH3 determination, when all the enzymes necessary for the complete break-down of allantoin are present. Bacteria containing allantoinase but not urease will be negative in this test. Using high amide concentrations (4 mM) some doubtful results concerning the degradation of acetamide, benzamide, nicotinamide and pyrazinamide can be eliminated as could be demonstrated for different strains of mycobacteria.", "contents": "Biochemical background of some enzymatic tests used for the differentiation of mycobacteria. The break-down of benzamide, acetamide, malonamide and allantoin in M. smegmatis was investigated. It has been stated that the uptake of liberated NH3 into the cells, favoured by the presence of an organic acid, occasionally results in a negative NH3 determination. This difficulty can be overcome by an increase of the substrate concentration from 0.8 up to 4 mM. All antoinase activity in mycobacteria can be demonstrated only by an NH3 determination, when all the enzymes necessary for the complete break-down of allantoin are present. Bacteria containing allantoinase but not urease will be negative in this test. Using high amide concentrations (4 mM) some doubtful results concerning the degradation of acetamide, benzamide, nicotinamide and pyrazinamide can be eliminated as could be demonstrated for different strains of mycobacteria.", "PMID": 960226}
{"id": "pubmed23n0032_3836", "title": "Differences in biochemical tests performed on photochromogenic mycobacteria isolated from human sources.", "content": "The photochromogenic mycobacteria do not all belong to one homogeneous species. With a simple heat-stable esterase (HSE) test it is possible to divide photochromogenic mycobacteria into two groups: HSE-positive--strongly pathogenic strains; and HSE-negative--weakly pathogenic strains. HSE-positive strains are mostly associated with pulmonary disease. HSE-negative strains are seldom associated with pulmonary disease, but are often associated with renal disease. It is rather difficult to draw a clear dividing line between pathogenic and non-pathogenic photochromogenic mycobacteria; and such a distinction probably does not exist in nature.", "contents": "Differences in biochemical tests performed on photochromogenic mycobacteria isolated from human sources. The photochromogenic mycobacteria do not all belong to one homogeneous species. With a simple heat-stable esterase (HSE) test it is possible to divide photochromogenic mycobacteria into two groups: HSE-positive--strongly pathogenic strains; and HSE-negative--weakly pathogenic strains. HSE-positive strains are mostly associated with pulmonary disease. HSE-negative strains are seldom associated with pulmonary disease, but are often associated with renal disease. It is rather difficult to draw a clear dividing line between pathogenic and non-pathogenic photochromogenic mycobacteria; and such a distinction probably does not exist in nature.", "PMID": 960227}
{"id": "pubmed23n0032_3837", "title": "Observations on the reduction of the renal elimination of urate in man caused by the administration of pyrazinamide.", "content": "The urinary excretion of pyrazinamide, pyrazinoic acid, 5-hydroxypyrazinoic acid and uric acid were determined in a healthy subject after giving single or multiple doses of pyrazinamide or its metabolite pyrazinoic acid. The results obtained demonstrated that 5-hydroxypyrazinoic acid is a major metabolite of pyrazinoic acid in man and supported previous evidence indicating that the retention of uric acid caused by the administration of pyrazinamide is mediated by pyrazinoic acid. After giving 3 g pyrazinamide the urinary excretion of uric acid was maximally suppressed for 24 hours and partially reduced for a further 24 hours. Prolonged exposure to pyrazinoic acid resulted in a net reduction in the urinary excretion of uric acid. The findings suggested that the degree of uric acid retention in patients treated with pyrazinamide-containing regimens could be reduced by giving pyrazinamide intermittently.", "contents": "Observations on the reduction of the renal elimination of urate in man caused by the administration of pyrazinamide. The urinary excretion of pyrazinamide, pyrazinoic acid, 5-hydroxypyrazinoic acid and uric acid were determined in a healthy subject after giving single or multiple doses of pyrazinamide or its metabolite pyrazinoic acid. The results obtained demonstrated that 5-hydroxypyrazinoic acid is a major metabolite of pyrazinoic acid in man and supported previous evidence indicating that the retention of uric acid caused by the administration of pyrazinamide is mediated by pyrazinoic acid. After giving 3 g pyrazinamide the urinary excretion of uric acid was maximally suppressed for 24 hours and partially reduced for a further 24 hours. Prolonged exposure to pyrazinoic acid resulted in a net reduction in the urinary excretion of uric acid. The findings suggested that the degree of uric acid retention in patients treated with pyrazinamide-containing regimens could be reduced by giving pyrazinamide intermittently.", "PMID": 960228}
{"id": "pubmed23n0032_3838", "title": "[Change in the hexokinase activity of the rat myocardial and skeletal muscle mitochondria and cytoplasm in fasting and on a carbohydrate diet].", "content": "Dynamics of the hexokinase activity was stuided in mitochondria and cytoplasm of skeletal muscle and myocardium in rats which were subjected to fasting for three days and then were given a carbohydrate diet. It is established that the hexokinase activity in mitochondria (especially in the miocardium) lowers during fasting to a less extent as compared to cytoplasm and is not restored when feeding on carbohydrates, evidently, due to different quantitative ratio of isoenzymes. It is shown that hexokinase from the myocardium solubilizes more easily that from the skeletal muscles.", "contents": "[Change in the hexokinase activity of the rat myocardial and skeletal muscle mitochondria and cytoplasm in fasting and on a carbohydrate diet]. Dynamics of the hexokinase activity was stuided in mitochondria and cytoplasm of skeletal muscle and myocardium in rats which were subjected to fasting for three days and then were given a carbohydrate diet. It is established that the hexokinase activity in mitochondria (especially in the miocardium) lowers during fasting to a less extent as compared to cytoplasm and is not restored when feeding on carbohydrates, evidently, due to different quantitative ratio of isoenzymes. It is shown that hexokinase from the myocardium solubilizes more easily that from the skeletal muscles.", "PMID": 960235}
{"id": "pubmed23n0032_3839", "title": "[Effect of hypoxia on mitochondrial stability].", "content": "The mitochondria isolated from the liver of rats subjected to acute hypoxia (10 km, 2h) are established to 0e less stable to the damage effect of incubation in the substratefree medium than the mitochondria of the control animals. A long interrupted adaptation to hypoxia as well as a single introduciton of p-chlorophenoxyacetic acid diethylaminoethylamide prevents a decrease in stability of the mitochondrial structures which is observed in rats during hypoxia. Addition of p-chlorophenoxyacetic acid diethylaminoethylamide into the incubation medium also inhibits the damage of the liver mitochondria.", "contents": "[Effect of hypoxia on mitochondrial stability]. The mitochondria isolated from the liver of rats subjected to acute hypoxia (10 km, 2h) are established to 0e less stable to the damage effect of incubation in the substratefree medium than the mitochondria of the control animals. A long interrupted adaptation to hypoxia as well as a single introduciton of p-chlorophenoxyacetic acid diethylaminoethylamide prevents a decrease in stability of the mitochondrial structures which is observed in rats during hypoxia. Addition of p-chlorophenoxyacetic acid diethylaminoethylamide into the incubation medium also inhibits the damage of the liver mitochondria.", "PMID": 960236}
{"id": "pubmed23n0032_3840", "title": "[Monamine oxidase activity of the brain and liver mitochondria under the action of high-pressure oxygen on animals].", "content": "The monoamine oxidase (MAO) activity with tryptamine, 5-hydroxytryptamine and noreponephrine as substrates was studied as affected by different conditions of hyperbaric oxygenation. Exposure of rats to oxygenation at 1 ati resulted in a 75% activation of the brain mitochondria. MAO activity with norepinephrine and in an insignificant increase in the activity with the other substrates. Oxygenation at 2,4 and 6 ati decreased the brain MAO activity with all the substrates. The reduciton in the activity correlated with oxygen tension increase and with exposure time. The MAO 100% inhibition observed in some animals at 4 ati and ain most animals at 6 ati. In the liver the activity changes to a less extent than in the brain. At 1 ati it is somewhat increased and at 2, 4 and 6 ati it decreases.", "contents": "[Monamine oxidase activity of the brain and liver mitochondria under the action of high-pressure oxygen on animals]. The monoamine oxidase (MAO) activity with tryptamine, 5-hydroxytryptamine and noreponephrine as substrates was studied as affected by different conditions of hyperbaric oxygenation. Exposure of rats to oxygenation at 1 ati resulted in a 75% activation of the brain mitochondria. MAO activity with norepinephrine and in an insignificant increase in the activity with the other substrates. Oxygenation at 2,4 and 6 ati decreased the brain MAO activity with all the substrates. The reduciton in the activity correlated with oxygen tension increase and with exposure time. The MAO 100% inhibition observed in some animals at 4 ati and ain most animals at 6 ati. In the liver the activity changes to a less extent than in the brain. At 1 ati it is somewhat increased and at 2, 4 and 6 ati it decreases.", "PMID": 960237}
{"id": "pubmed23n0032_3841", "title": "[Pentosephosphate shunt enzymatic activity in the liver in its disordered innervation].", "content": "Partial denervation of the liver evoked the dissection of subphrenic vagus nerves and nerves of the hepatogastric duodenal ligament tissue stimulates synthesis of pentoses in the liver due to the oxidative mechanism and inhibits the process of unoxidative formation of pentoses. Three-four weeks after the operation one may observe a 72% increase in the activity of glucose-6-phosphate dehydrogenase in the liver tissue and a 29 and 21% drop in the activity of transketolase and enzymes, respectively, participating in metabolism of ribose-5-phosphate. The content of soluble proteins in the liver with deneravation is unchanged. A correlation is observed between these changes and those in the content of nucleic acids, an opinion is confirmed on the autonomous character of the oxidative and unoxidative phases of the pentose cycle.", "contents": "[Pentosephosphate shunt enzymatic activity in the liver in its disordered innervation]. Partial denervation of the liver evoked the dissection of subphrenic vagus nerves and nerves of the hepatogastric duodenal ligament tissue stimulates synthesis of pentoses in the liver due to the oxidative mechanism and inhibits the process of unoxidative formation of pentoses. Three-four weeks after the operation one may observe a 72% increase in the activity of glucose-6-phosphate dehydrogenase in the liver tissue and a 29 and 21% drop in the activity of transketolase and enzymes, respectively, participating in metabolism of ribose-5-phosphate. The content of soluble proteins in the liver with deneravation is unchanged. A correlation is observed between these changes and those in the content of nucleic acids, an opinion is confirmed on the autonomous character of the oxidative and unoxidative phases of the pentose cycle.", "PMID": 960238}
{"id": "pubmed23n0032_3842", "title": "[Effect of hypothermia and hyperoxia on the ammonia-glutamic acid system in the brain of rats].", "content": "The content of ammonia, glutamine, dicarboxylic amino acids and GABA was studied in the brain under 1, 2, 4-fold separate and simultaneous effect of hypothermia (19-20 C) and hyperoxia (3 atm.). A two-fold hypothermia of rats is accompanied by a greater increase of ammonia in the brain than a three-fold one. The content of glutamine under two-fold cooling is unchanged and under three-fold cooling it is twice as low as compared to its content in the brain of the control rats. The content of glutamic acid decreased after two-fold hypothermia is almost unchanged by the third seance of hypothermia. The repeated actions of hyperoxia also cause a considerable increase in the ammonia content but the dynamics of changes in the content of the nitrogenous metabolic products is contary to that in animals subjected to repeated seances of hypothermia. A simultaneous combined action of hypothermia and hyperoxia produces no additive effect on the system ammonia-glutaminic acid.", "contents": "[Effect of hypothermia and hyperoxia on the ammonia-glutamic acid system in the brain of rats]. The content of ammonia, glutamine, dicarboxylic amino acids and GABA was studied in the brain under 1, 2, 4-fold separate and simultaneous effect of hypothermia (19-20 C) and hyperoxia (3 atm.). A two-fold hypothermia of rats is accompanied by a greater increase of ammonia in the brain than a three-fold one. The content of glutamine under two-fold cooling is unchanged and under three-fold cooling it is twice as low as compared to its content in the brain of the control rats. The content of glutamic acid decreased after two-fold hypothermia is almost unchanged by the third seance of hypothermia. The repeated actions of hyperoxia also cause a considerable increase in the ammonia content but the dynamics of changes in the content of the nitrogenous metabolic products is contary to that in animals subjected to repeated seances of hypothermia. A simultaneous combined action of hypothermia and hyperoxia produces no additive effect on the system ammonia-glutaminic acid.", "PMID": 960239}
{"id": "pubmed23n0032_3843", "title": "[Role of the blood bicarbonate buffer system in the mechanism of fish adaptation to different levels of carbonic acid in an aqueous medium].", "content": "The role of a bicarbonate buffer system of fish (Cyprinidae family) blood was studied in their organism addaptive reactions to different levels of CO2 in the aqueous medium. The fish is established to prossess rather effective for maintaining blood acid-base balance. It permits the fish to endure for a long time essential fluctuations of carbonic acid concentration in water. In prevention of possible development of carbonic acid acidosis an essential role belongs to formation of bicarbonates as a blood buffer system stablizing pH is shown to be significant for preventing possible development of acidosis. The adaptation potentialities of Cyprinidae family permit them to endure an increase of CO2 in water and are determined by the ability of their organism to formations of bicarbonate and their retaining in blood.", "contents": "[Role of the blood bicarbonate buffer system in the mechanism of fish adaptation to different levels of carbonic acid in an aqueous medium]. The role of a bicarbonate buffer system of fish (Cyprinidae family) blood was studied in their organism addaptive reactions to different levels of CO2 in the aqueous medium. The fish is established to prossess rather effective for maintaining blood acid-base balance. It permits the fish to endure for a long time essential fluctuations of carbonic acid concentration in water. In prevention of possible development of carbonic acid acidosis an essential role belongs to formation of bicarbonates as a blood buffer system stablizing pH is shown to be significant for preventing possible development of acidosis. The adaptation potentialities of Cyprinidae family permit them to endure an increase of CO2 in water and are determined by the ability of their organism to formations of bicarbonate and their retaining in blood.", "PMID": 960242}
{"id": "pubmed23n0032_3844", "title": "[Carboxylation intensity in the liver of birds of different ages].", "content": "Carboxylation intensity was studied in the liver of young and adult cocks, 14C-bi-carbonate was used as an indicator of the amino acids, proteins and lipids turnover. It is established that the amount of dry residue in the liver tissue of the adult cocks is 22% higher and that of pyruvate and dicarboxylic amino acids is twice as low. The relative weight of the liver changes with age: in the 18-month-old cocks it is 1.5%, in the one-month-old cocks, 2.75%. The maximum of 14C incorporation into the tissue, dicarboxylic compounds, proteins and lipids of young cocks in the in vivo experiments is at a shorter exposure of the isotope. 14C removal from these compounds in the young cocks occurs more quickly as well. A higher radioactivity of the compounds under study in the adult cocks liver with prolongation of the isotope exposure is due to the effect of factors which are not related to the synthesis. The experiments established that radioactivity of proteins and lipids in the young cock liver slices is 6 times as high at 60-minute exposure. The mentioned above evidences for a higher rate of carboxylation in young cocks as compared to adult ones. When interpreting the data of metabolism study by means of isotopes in animals of different age a corrections should be made for a change in the liver relative weight during the growth process.", "contents": "[Carboxylation intensity in the liver of birds of different ages]. Carboxylation intensity was studied in the liver of young and adult cocks, 14C-bi-carbonate was used as an indicator of the amino acids, proteins and lipids turnover. It is established that the amount of dry residue in the liver tissue of the adult cocks is 22% higher and that of pyruvate and dicarboxylic amino acids is twice as low. The relative weight of the liver changes with age: in the 18-month-old cocks it is 1.5%, in the one-month-old cocks, 2.75%. The maximum of 14C incorporation into the tissue, dicarboxylic compounds, proteins and lipids of young cocks in the in vivo experiments is at a shorter exposure of the isotope. 14C removal from these compounds in the young cocks occurs more quickly as well. A higher radioactivity of the compounds under study in the adult cocks liver with prolongation of the isotope exposure is due to the effect of factors which are not related to the synthesis. The experiments established that radioactivity of proteins and lipids in the young cock liver slices is 6 times as high at 60-minute exposure. The mentioned above evidences for a higher rate of carboxylation in young cocks as compared to adult ones. When interpreting the data of metabolism study by means of isotopes in animals of different age a corrections should be made for a change in the liver relative weight during the growth process.", "PMID": 960241}
{"id": "pubmed23n0032_3845", "title": "[Effect of glutamine, thymidine and orotate on the nucleic acid content in the cell nuclei of the regenerating liver in sodium fluoride poisoning].", "content": "Sodium fluoride inhibits an increase of nucleic acids in the cells nuclei of the rat liver after a partial resection of this organ. Introduction of thymidine to the animals poisoned with sodium fluoride, contrary to glutamine and orotate, causes a sharp decrease in the amount of nuclei in 1 mg of tissue and normalization of the nucleic acid content in them. Changes in the mitotic activity may be connected with a disturbance in the normal ratio of the nucleic acids synthesis precursors.", "contents": "[Effect of glutamine, thymidine and orotate on the nucleic acid content in the cell nuclei of the regenerating liver in sodium fluoride poisoning]. Sodium fluoride inhibits an increase of nucleic acids in the cells nuclei of the rat liver after a partial resection of this organ. Introduction of thymidine to the animals poisoned with sodium fluoride, contrary to glutamine and orotate, causes a sharp decrease in the amount of nuclei in 1 mg of tissue and normalization of the nucleic acid content in them. Changes in the mitotic activity may be connected with a disturbance in the normal ratio of the nucleic acids synthesis precursors.", "PMID": 960243}
{"id": "pubmed23n0032_3846", "title": "[Fructose content and aldolase activity in the tissues of chickens under ultraviolet irradiation].", "content": "The effect of UV-irradiation was studied as applied to the content of fructose and activity of aldolase in the liver tissue and muscles of chickens and embryos obtained from eggs eight months after the chicken irradiation. It is established that the content of fructose and activity of aldolase in the tissues of 19-day embryos are approximately the same as in 40-140 day chickens. Under the effect of UV-irradiation the content of fructose in the liver increases in 140-day chickens and in embryos of the group under experiment, and it decreases in the muscular tissue of 80-day chickens. In the liver of embryos obtained from the irradiated bird the activity of aldolase lowers and in young chickens no essential difference is observed between the groups. The value of the ratio of the aldolase activity for both substrates in the liver of embryos and chickens of the group under study at the age of 40 and 140 days is somewhat higher than in the control, and in the muscular tissue it is lower (in embryos and chickens at the age of 40- and 80-days).", "contents": "[Fructose content and aldolase activity in the tissues of chickens under ultraviolet irradiation]. The effect of UV-irradiation was studied as applied to the content of fructose and activity of aldolase in the liver tissue and muscles of chickens and embryos obtained from eggs eight months after the chicken irradiation. It is established that the content of fructose and activity of aldolase in the tissues of 19-day embryos are approximately the same as in 40-140 day chickens. Under the effect of UV-irradiation the content of fructose in the liver increases in 140-day chickens and in embryos of the group under experiment, and it decreases in the muscular tissue of 80-day chickens. In the liver of embryos obtained from the irradiated bird the activity of aldolase lowers and in young chickens no essential difference is observed between the groups. The value of the ratio of the aldolase activity for both substrates in the liver of embryos and chickens of the group under study at the age of 40 and 140 days is somewhat higher than in the control, and in the muscular tissue it is lower (in embryos and chickens at the age of 40- and 80-days).", "PMID": 960240}
{"id": "pubmed23n0032_3847", "title": "[Separation of thiamine phosphoric esters on cation-exchange Saphadex].", "content": "A new method is suggested for separation of thiamine phosphoric esters (TPE) from biological materials. The aliquote of evaporated extract from tissue was applied to the column with Sephadex SE-C-25 (25X2.5), TTP passed through Sephadex with 0,005 M acetate buffer, pH 3.8-4.0, TDP was eluated with 0,05 M acetate buffer; TMP and T--1 M NaC1 in 1.0 M acetate buffer. Yield of each phosphoric ester was about 100%. The method allows separating thiamine phosphoric esters without preliminary purification of charcoal. It may be preferable to determine the content of TTP in tissue.", "contents": "[Separation of thiamine phosphoric esters on cation-exchange Saphadex]. A new method is suggested for separation of thiamine phosphoric esters (TPE) from biological materials. The aliquote of evaporated extract from tissue was applied to the column with Sephadex SE-C-25 (25X2.5), TTP passed through Sephadex with 0,005 M acetate buffer, pH 3.8-4.0, TDP was eluated with 0,05 M acetate buffer; TMP and T--1 M NaC1 in 1.0 M acetate buffer. Yield of each phosphoric ester was about 100%. The method allows separating thiamine phosphoric esters without preliminary purification of charcoal. It may be preferable to determine the content of TTP in tissue.", "PMID": 960244}
{"id": "pubmed23n0032_3848", "title": "Influence of sympathetic nerve activity on renal haemodynamics and release of renin. A preliminary communication.", "content": "The role of the sympathetic nerve system is still unknown in different types of hypertension. The procedure used in the evaluation of suspect renovascular hypertension has been revised in such a way that renal blood flow, renal arterial blood pressure and plasma renin activity in renal vein could be determined prior to and after splanchnic blockade. A new divice-videovolumeter-has been used to investigate the blood flow. Some preliminary data are given.", "contents": "Influence of sympathetic nerve activity on renal haemodynamics and release of renin. A preliminary communication. The role of the sympathetic nerve system is still unknown in different types of hypertension. The procedure used in the evaluation of suspect renovascular hypertension has been revised in such a way that renal blood flow, renal arterial blood pressure and plasma renin activity in renal vein could be determined prior to and after splanchnic blockade. A new divice-videovolumeter-has been used to investigate the blood flow. Some preliminary data are given.", "PMID": 960290}
{"id": "pubmed23n0032_3849", "title": "Segmental arterial spasm in patients with total brain infarction.", "content": "An arteriographic investigation has shown that segmental spasm occurs in a relatively high frequency of patients with total brain infarction (12 of 30), and if spasm at the origin of arterial branches is included, the frequency is still higher (19 of 30). The phenomenon is possibly a sign of changed sympathetic tone, the pathophysiological significance of which is discussed.", "contents": "Segmental arterial spasm in patients with total brain infarction. An arteriographic investigation has shown that segmental spasm occurs in a relatively high frequency of patients with total brain infarction (12 of 30), and if spasm at the origin of arterial branches is included, the frequency is still higher (19 of 30). The phenomenon is possibly a sign of changed sympathetic tone, the pathophysiological significance of which is discussed.", "PMID": 960291}
{"id": "pubmed23n0032_3850", "title": "Arterial venography of the dog's leg.", "content": "Good visualization of the veins of the leg in the dog were obtained simply by increasing the dose to ca. 2 ml per kg tissue weight of contrast agent containing 282-300 mg I/ml. This is equivalent to the dose used in celiac, cerebral and coronary arteriography in man. Moderate increase in flow induced by injection of roentgen contrast agents gave some improvement in the visualization  of the veins. Infusions of bradykinin and attempts at reactive hyperemia did not improve the visualization of the veins.", "contents": "Arterial venography of the dog's leg. Good visualization of the veins of the leg in the dog were obtained simply by increasing the dose to ca. 2 ml per kg tissue weight of contrast agent containing 282-300 mg I/ml. This is equivalent to the dose used in celiac, cerebral and coronary arteriography in man. Moderate increase in flow induced by injection of roentgen contrast agents gave some improvement in the visualization  of the veins. Infusions of bradykinin and attempts at reactive hyperemia did not improve the visualization of the veins.", "PMID": 960292}
{"id": "pubmed23n0032_3851", "title": "Relation between erythrocyte and plasma lithium concentrations as an index in psychiatric disease.", "content": "In longitudinal and transverse studies, lithium was measured in plasma, serum and red blood cells (erythrocyte) of healthy male and female subjects as well as in patients of both sexes suffering from manic-depressive disease or schizophrenia. The results confirm that lithium in erythrocytes is lower than in plasma in all groups. The lithium concentration gradient between plasma and erythrocytes is not caused by a slow rate of diffusion through the erythrocyte membrane. The new result of the present study is the importance of sex, disease and age on the erythrocyte/plasma lithium ratio, which is significantly higher in female subjects with manic-depressive disease. This difference persists even during long-term lithium therapy. Older female schizophrenics also have a higher ratio of erythrocyte to plasma lithium than males of the same age. The findings emphasize the importance of endocrine investigation in mental disease and support the view that plasma lithium in humans does not always reflect the intracellular levels. The erythrocyte plasma ratio may also be of value in revealing diagnostic subgroups within the classical psychiatric framework.", "contents": "Relation between erythrocyte and plasma lithium concentrations as an index in psychiatric disease. In longitudinal and transverse studies, lithium was measured in plasma, serum and red blood cells (erythrocyte) of healthy male and female subjects as well as in patients of both sexes suffering from manic-depressive disease or schizophrenia. The results confirm that lithium in erythrocytes is lower than in plasma in all groups. The lithium concentration gradient between plasma and erythrocytes is not caused by a slow rate of diffusion through the erythrocyte membrane. The new result of the present study is the importance of sex, disease and age on the erythrocyte/plasma lithium ratio, which is significantly higher in female subjects with manic-depressive disease. This difference persists even during long-term lithium therapy. Older female schizophrenics also have a higher ratio of erythrocyte to plasma lithium than males of the same age. The findings emphasize the importance of endocrine investigation in mental disease and support the view that plasma lithium in humans does not always reflect the intracellular levels. The erythrocyte plasma ratio may also be of value in revealing diagnostic subgroups within the classical psychiatric framework.", "PMID": 960293}
{"id": "pubmed23n0032_3852", "title": "Changes of reactive hyperaemia after clinical bed rest for seven days.", "content": "As an indication of peripheral circulatory function reactive hyperaemia was studied in the forearm and calf muscle in 14 healthy young men before and after clinical bed rest for one week. Blood flow was measured after different arterial occlusion times with venous occlusion plethysmography. After bed rest peak flow values in the calf after arterial occlusion for 3 or 5 minutes decreased moderately (by about 20-23%) and significantly. Peak flow in the forearm decreased as well although not significantly.", "contents": "Changes of reactive hyperaemia after clinical bed rest for seven days. As an indication of peripheral circulatory function reactive hyperaemia was studied in the forearm and calf muscle in 14 healthy young men before and after clinical bed rest for one week. Blood flow was measured after different arterial occlusion times with venous occlusion plethysmography. After bed rest peak flow values in the calf after arterial occlusion for 3 or 5 minutes decreased moderately (by about 20-23%) and significantly. Peak flow in the forearm decreased as well although not significantly.", "PMID": 960294}
{"id": "pubmed23n0032_3853", "title": "Gastric evacuation and propulsive intestinal motility in acute afferent loop syndrome in the rat.", "content": "The acute afferent loop syndrome, i.e. occlusion of the afferent loop after partial gastrectomy by the Billroth II method, was produced in the rat. In a primary session a gastrojejunostomy with division of the pylorus was performed. 2-3 months later the afferent loop was ligated. The gastric evacuation and the propulsive motility of the intestine were studied quantitatively, using an inert radioisotope. Both the gastric evacuation and the propulsive intestinal motility were considerably delayed in ALS, both in relation to the laparotomized controls and in relation to previous findings in mechanical intestinal obstruction and paralytic ileus due to retroperitoneal irritation or bacterial peritonitis.", "contents": "Gastric evacuation and propulsive intestinal motility in acute afferent loop syndrome in the rat. The acute afferent loop syndrome, i.e. occlusion of the afferent loop after partial gastrectomy by the Billroth II method, was produced in the rat. In a primary session a gastrojejunostomy with division of the pylorus was performed. 2-3 months later the afferent loop was ligated. The gastric evacuation and the propulsive motility of the intestine were studied quantitatively, using an inert radioisotope. Both the gastric evacuation and the propulsive intestinal motility were considerably delayed in ALS, both in relation to the laparotomized controls and in relation to previous findings in mechanical intestinal obstruction and paralytic ileus due to retroperitoneal irritation or bacterial peritonitis.", "PMID": 960296}
{"id": "pubmed23n0032_3854", "title": "[Kidney function test after partial nephrectomy (author's transl)].", "content": "A new method for testing the function of the kidney after partial nephrectomy is reported. By means of radioisotope renography, urography and radioisotope clearance ('single-shot' method) it is possible to prove the function of the single kidney. The clearance in regard to the size of the shadow of the kidney assessed by urography allows to estimate the specified kidney function. We used these methods in ten patients and we detected that the loss of function is more extensive than expected by the operative reduction of the kidney parenchyma only. The causes for this are discussed.", "contents": "[Kidney function test after partial nephrectomy (author's transl)]. A new method for testing the function of the kidney after partial nephrectomy is reported. By means of radioisotope renography, urography and radioisotope clearance ('single-shot' method) it is possible to prove the function of the single kidney. The clearance in regard to the size of the shadow of the kidney assessed by urography allows to estimate the specified kidney function. We used these methods in ten patients and we detected that the loss of function is more extensive than expected by the operative reduction of the kidney parenchyma only. The causes for this are discussed.", "PMID": 960297}
{"id": "pubmed23n0032_3855", "title": "[Urinary continence after radical prostatectomy: the urodynamic proof of an anatomical hypothesis (author's transl)].", "content": "Prostatic carcinoma can be cured by a radical operation. In radical prostatectomy the whole so-called internal sphincter is removed. It is generally accepted and often proved that the external sphincter which is mostly somatically innervated cannot resume the funciton of continence. The postoperative urinary incontinence after radical prostatectomy is frequent and therefore the radical prostatectomy is not performed in many places. The problem of postoperative continence as well as incontinence after enucleation of the adenoma will be briefly discussed. In 1966, HUTCH described the so-called 'baseplate'. It is a bundle of smooth muscle fibers, circulary arranged around the bladder neck, which are also responsible for passive continence. He proposed that a certain sphincter function can be achieved by a tubular anastomosis of this anatomical substratum with the distal urethral stump. We were able to prove this anatomical hypothesis urodynamically. A specially constructed double-lumen perfusion catheter enabled us to register the so-called functional urethra profile and simultaneously register a new sphincter pressure wave in the transformed 'baseplate' which was responsible for the continence. We could observe this new sphincter region in all continent patients after radical prostatectomy whereas in the incontinent patients it was absent. Since 1972 we have employed the procedure proposed by HUTCH and have performed 20 radical prostatectomies. All these patients have been postoperatively completely continent. We are of the opinion that undue restraint concerning radical prostatectomy is no more justified.", "contents": "[Urinary continence after radical prostatectomy: the urodynamic proof of an anatomical hypothesis (author's transl)]. Prostatic carcinoma can be cured by a radical operation. In radical prostatectomy the whole so-called internal sphincter is removed. It is generally accepted and often proved that the external sphincter which is mostly somatically innervated cannot resume the funciton of continence. The postoperative urinary incontinence after radical prostatectomy is frequent and therefore the radical prostatectomy is not performed in many places. The problem of postoperative continence as well as incontinence after enucleation of the adenoma will be briefly discussed. In 1966, HUTCH described the so-called 'baseplate'. It is a bundle of smooth muscle fibers, circulary arranged around the bladder neck, which are also responsible for passive continence. He proposed that a certain sphincter function can be achieved by a tubular anastomosis of this anatomical substratum with the distal urethral stump. We were able to prove this anatomical hypothesis urodynamically. A specially constructed double-lumen perfusion catheter enabled us to register the so-called functional urethra profile and simultaneously register a new sphincter pressure wave in the transformed 'baseplate' which was responsible for the continence. We could observe this new sphincter region in all continent patients after radical prostatectomy whereas in the incontinent patients it was absent. Since 1972 we have employed the procedure proposed by HUTCH and have performed 20 radical prostatectomies. All these patients have been postoperatively completely continent. We are of the opinion that undue restraint concerning radical prostatectomy is no more justified.", "PMID": 960298}
{"id": "pubmed23n0032_3856", "title": "[Treatment of urinary tract infections caused by unknown bacteria (author's transl)].", "content": "The treatment of acute febrile urinary tract infections in urological patients has to start in most cases without knowledge of bacteria and their sensitivity. The results of 2,000 antibiograms and clinical observations showed good efficiency in initial therapy if combined treatment with gentamycin and cephalothin was used. Using recommeneded doses in permanent control of serum creatinine and blood-urea nitrogen, no decrease of renal function was observed in 131 patients treated with this combined therapy.", "contents": "[Treatment of urinary tract infections caused by unknown bacteria (author's transl)]. The treatment of acute febrile urinary tract infections in urological patients has to start in most cases without knowledge of bacteria and their sensitivity. The results of 2,000 antibiograms and clinical observations showed good efficiency in initial therapy if combined treatment with gentamycin and cephalothin was used. Using recommeneded doses in permanent control of serum creatinine and blood-urea nitrogen, no decrease of renal function was observed in 131 patients treated with this combined therapy.", "PMID": 960299}
{"id": "pubmed23n0032_3857", "title": "The adreno-renal-ureteral sheath: surgical-anatomical study.", "content": "The adreno-renal-ureteral sheath is described, showing the continuity of the anterior and posterior leaves of the sheath along the entire length of the retroperitoneum of each side. The study is based on anatomical dissections and on surgical observations. Anatomical and pathological considerations are discussed.", "contents": "The adreno-renal-ureteral sheath: surgical-anatomical study. The adreno-renal-ureteral sheath is described, showing the continuity of the anterior and posterior leaves of the sheath along the entire length of the retroperitoneum of each side. The study is based on anatomical dissections and on surgical observations. Anatomical and pathological considerations are discussed.", "PMID": 960300}
{"id": "pubmed23n0032_3858", "title": "[Experience with the Cryosurgical treatment of the prostatic diseases in 100 patients (author's transl)].", "content": "Cryosurgery of the prostate should be performed only in high risk patients on which other conventional methods of treatment cannot be applied. 82% of the patients could void after cryosurgery without indwelling catheter. Cryosurgery of the prostate became one of the routine procedures in the treatment of the prostatic diseases.", "contents": "[Experience with the Cryosurgical treatment of the prostatic diseases in 100 patients (author's transl)]. Cryosurgery of the prostate should be performed only in high risk patients on which other conventional methods of treatment cannot be applied. 82% of the patients could void after cryosurgery without indwelling catheter. Cryosurgery of the prostate became one of the routine procedures in the treatment of the prostatic diseases.", "PMID": 960301}
{"id": "pubmed23n0032_3859", "title": "[Lyophilized pig bladder as a partial bladder wall substitute (author's transl)].", "content": "Lyophilized pig bladder was used as partial replacement of the urinary bladder wall on 22 rabbits. This material is not suitable for this purpose. Lyophilized pig bladder shows no advantages compared to human dura as a bladder wall substitute.", "contents": "[Lyophilized pig bladder as a partial bladder wall substitute (author's transl)]. Lyophilized pig bladder was used as partial replacement of the urinary bladder wall on 22 rabbits. This material is not suitable for this purpose. Lyophilized pig bladder shows no advantages compared to human dura as a bladder wall substitute.", "PMID": 960302}
{"id": "pubmed23n0032_3860", "title": "[Grouping of urinary stones and exactness of analyses (author's transl)].", "content": "The composition of 1,327 urinary stones by X-ray diffraction is statistically analyzed, and the exact analyses are discussed. The representative test portion of the urinary stone is important to the result. A comparison of different results of urinary stones with that of other authors is only possible when we know the exactness of the method.", "contents": "[Grouping of urinary stones and exactness of analyses (author's transl)]. The composition of 1,327 urinary stones by X-ray diffraction is statistically analyzed, and the exact analyses are discussed. The representative test portion of the urinary stone is important to the result. A comparison of different results of urinary stones with that of other authors is only possible when we know the exactness of the method.", "PMID": 960303}
{"id": "pubmed23n0032_3861", "title": "[Micturitional disorders in multiple sclerosis (author's transl)].", "content": "Micturitional disorders in multiple sclerosis have often an insidious onset; they aggravate during acute attacks of the nervous disease and are strongly influenced by the general condition of the patient, the evolution of his muscular spasms and the therapeutical measures. This changing behaviour of the bladder makes it necessary to perform repeated cystometries, uroflowmetries and electromyographies of the striated sphincter in order to adapt vesical therapy to the evolutive stadia of the disease. Although good results are frequently obtained by drugs (sympathicomimetics, sympathicolytics, muscle relaxants, parasympathicomimetics, parasympathicolytics), by transurethral resections of internal and/or external sphincters and by phenolisation, we feel that urinary derivations remain indicated for some patients, especially in order to facilitate social integration.", "contents": "[Micturitional disorders in multiple sclerosis (author's transl)]. Micturitional disorders in multiple sclerosis have often an insidious onset; they aggravate during acute attacks of the nervous disease and are strongly influenced by the general condition of the patient, the evolution of his muscular spasms and the therapeutical measures. This changing behaviour of the bladder makes it necessary to perform repeated cystometries, uroflowmetries and electromyographies of the striated sphincter in order to adapt vesical therapy to the evolutive stadia of the disease. Although good results are frequently obtained by drugs (sympathicomimetics, sympathicolytics, muscle relaxants, parasympathicomimetics, parasympathicolytics), by transurethral resections of internal and/or external sphincters and by phenolisation, we feel that urinary derivations remain indicated for some patients, especially in order to facilitate social integration.", "PMID": 960304}
{"id": "pubmed23n0032_3862", "title": "[Clinical and genetic aspects of the horseshoe kidney (author's transl)].", "content": "The horseshoe kidney is a not so rare fusion anomaly of the urinary tract system. Clinical and genetic aspects of this phenomenon are shown in a case of familiar occurrence. Genetic consultation of the family must consider various inheritable possibilities and sources of this disease. The difficulty of an exact genealogical tree analysis is pointed out.", "contents": "[Clinical and genetic aspects of the horseshoe kidney (author's transl)]. The horseshoe kidney is a not so rare fusion anomaly of the urinary tract system. Clinical and genetic aspects of this phenomenon are shown in a case of familiar occurrence. Genetic consultation of the family must consider various inheritable possibilities and sources of this disease. The difficulty of an exact genealogical tree analysis is pointed out.", "PMID": 960305}
{"id": "pubmed23n0032_3863", "title": "Pulmonary management of the urologic patient.", "content": "Morbidity and mortality from pulmonary complications following urinary tract operations can be reduced by preoperative identification of the high risk patient. Pulmonary function tests and arterial blood gases are necessary to identify these patients and to delineate the severity of their pulmonary disease. Respiratory complications can be prevented in many patients with the proper use of pre- and postoperative chest physical therapy and oxygen therapy. Despite the most careful pulmonary management, some patients develop acute respiratory failure following urologic operations. Respiratory failure results from a combination of physiologic abnormalities which impair alveolar ventilation and oxygenation. Utilizing controlled ventilation, supplemental oxygen, and a physiologic approach to treating the underlying cause of respiratory failure, three fourths of urologic patients in respiratory failure may be expected to survive.", "contents": "Pulmonary management of the urologic patient. Morbidity and mortality from pulmonary complications following urinary tract operations can be reduced by preoperative identification of the high risk patient. Pulmonary function tests and arterial blood gases are necessary to identify these patients and to delineate the severity of their pulmonary disease. Respiratory complications can be prevented in many patients with the proper use of pre- and postoperative chest physical therapy and oxygen therapy. Despite the most careful pulmonary management, some patients develop acute respiratory failure following urologic operations. Respiratory failure results from a combination of physiologic abnormalities which impair alveolar ventilation and oxygenation. Utilizing controlled ventilation, supplemental oxygen, and a physiologic approach to treating the underlying cause of respiratory failure, three fourths of urologic patients in respiratory failure may be expected to survive.", "PMID": 960309}
{"id": "pubmed23n0032_3864", "title": "The hematologic evaluation of the urologic patient.", "content": "This article provides a framework for the hematologic evaluation of the urology patient. The discussion is based on an analysis of test results obtained from routine screening laboratory studies as the complete blood count and platelet count. The use of automated blood counts also provides quantitative indices of red cell morphology which facilitate the diagnosis of red blood cell disorders. To aid in categorizing hematologic disorders, abnormalities of the red blood cells, white blood cells, and platelets are discussed separately. In some circumstances where a disease process, such as bacteremia, alters more than one of the formed elements of the blood, this speparate analysis approach is less appropriate. Nevertheless, this division is generally useful in arriving at the correct interpretation of hematologic abnormalities. Within each category of abnormal test results, a variety of etiologies is listed. Elaboration is reserved for the entities commonly seen in hospitalized patients. For a more detailed discussion of the common disorders or for a more inclusive listing of the less common disorders, the bibliography lists a selection of useful references.", "contents": "The hematologic evaluation of the urologic patient. This article provides a framework for the hematologic evaluation of the urology patient. The discussion is based on an analysis of test results obtained from routine screening laboratory studies as the complete blood count and platelet count. The use of automated blood counts also provides quantitative indices of red cell morphology which facilitate the diagnosis of red blood cell disorders. To aid in categorizing hematologic disorders, abnormalities of the red blood cells, white blood cells, and platelets are discussed separately. In some circumstances where a disease process, such as bacteremia, alters more than one of the formed elements of the blood, this speparate analysis approach is less appropriate. Nevertheless, this division is generally useful in arriving at the correct interpretation of hematologic abnormalities. Within each category of abnormal test results, a variety of etiologies is listed. Elaboration is reserved for the entities commonly seen in hospitalized patients. For a more detailed discussion of the common disorders or for a more inclusive listing of the less common disorders, the bibliography lists a selection of useful references.", "PMID": 960310}
{"id": "pubmed23n0032_3865", "title": "Pre- and postoperative renal failure.", "content": "The acute onset of oliguria and azotemia in the postoperative setting may be caused by pre-renal causes or intrinsic renal damage. The first step in arriving at a diagnosis is to review the history as noted above for clues regarding fluid balance, treatment with nephrotoxins, etc. The typical patient with prerenal azotemia will present with evidence of the recent onset of worsening of pre-existing cardiac disease, renal or gastrointestinal fluid loss, or the accumulation of acites, edema, or retroperitoneal fluid. In the absence of very recent diuretic therapy, he will be excreting a scant amount of concentrated (greater than 400 mOsm per L) sodium free (less than 10 to 20 mEq per L) urine. The serumBUN/Cr ratio is often greater than 15 to 20:1, and their urinary sediment will be bland. In an occasional patient in whom these studies give equivocal results, additional help may be obtained with measurements of central venous pressure (CVP) or pulmonary wedge pressure (PWP) and by noting their response to intravenous fluid loading. A rising CVP or PWP in the face of salt loading is, of course, evidence against prerenal azotemia. Patients with obstructive uropathies may be oligoanuric or polyuric-occasionally a characteristic alternating polyuria and oliguria is found (due to displacement of a stone or relief of edema). When oliguric their urine typically contains substantial amounts of sodium (greater than 20 mEq per L), is isotonic, and their OsmU:OsmP is les s than or equal to 1.2. Their urinary sediment will reflect the cause of their obstruction as noted above. A renal scan, ultrasound study, or infusion IVP are mandatory to rule out the possibility of obstructive uropathy. If these nonivasive studies are equivocal, one must consider doing a unilateral retrograde. The development of ATN usually occurs in the setting of hypotension, sepsis, dehydration, and with exposure to nephrotoxins. Most patients with be excreting scant amounts of isotonic urine containing more than 20 to 30 mEq per L of sodium. Their CrU:CrP is less than or equal to 20:1 and their urinary sediment reveals many epithelial cells and casts. Those patients with nonoliguric ATN have urine outputs which may exceed 2 liters per day. Despite this output they demonstrate a stepwise increase in serum urea and creatinine. Urine sodium and osmolality are not very helpful in this setting. Many such patients do have low (less than 20 mEg per L) urine sodium concentration and excrete isotonic urine.", "contents": "Pre- and postoperative renal failure. The acute onset of oliguria and azotemia in the postoperative setting may be caused by pre-renal causes or intrinsic renal damage. The first step in arriving at a diagnosis is to review the history as noted above for clues regarding fluid balance, treatment with nephrotoxins, etc. The typical patient with prerenal azotemia will present with evidence of the recent onset of worsening of pre-existing cardiac disease, renal or gastrointestinal fluid loss, or the accumulation of acites, edema, or retroperitoneal fluid. In the absence of very recent diuretic therapy, he will be excreting a scant amount of concentrated (greater than 400 mOsm per L) sodium free (less than 10 to 20 mEq per L) urine. The serumBUN/Cr ratio is often greater than 15 to 20:1, and their urinary sediment will be bland. In an occasional patient in whom these studies give equivocal results, additional help may be obtained with measurements of central venous pressure (CVP) or pulmonary wedge pressure (PWP) and by noting their response to intravenous fluid loading. A rising CVP or PWP in the face of salt loading is, of course, evidence against prerenal azotemia. Patients with obstructive uropathies may be oligoanuric or polyuric-occasionally a characteristic alternating polyuria and oliguria is found (due to displacement of a stone or relief of edema). When oliguric their urine typically contains substantial amounts of sodium (greater than 20 mEq per L), is isotonic, and their OsmU:OsmP is les s than or equal to 1.2. Their urinary sediment will reflect the cause of their obstruction as noted above. A renal scan, ultrasound study, or infusion IVP are mandatory to rule out the possibility of obstructive uropathy. If these nonivasive studies are equivocal, one must consider doing a unilateral retrograde. The development of ATN usually occurs in the setting of hypotension, sepsis, dehydration, and with exposure to nephrotoxins. Most patients with be excreting scant amounts of isotonic urine containing more than 20 to 30 mEq per L of sodium. Their CrU:CrP is less than or equal to 20:1 and their urinary sediment reveals many epithelial cells and casts. Those patients with nonoliguric ATN have urine outputs which may exceed 2 liters per day. Despite this output they demonstrate a stepwise increase in serum urea and creatinine. Urine sodium and osmolality are not very helpful in this setting. Many such patients do have low (less than 20 mEg per L) urine sodium concentration and excrete isotonic urine.", "PMID": 960314}
{"id": "pubmed23n0032_3866", "title": "Hazards to bladder and intestinal tissues from intravesical underwater electrical discharges from a surgical electronic lithoclast.", "content": "Previous studies have shown that electronic lithoclast discharges produce effects resembling an underwater explosion. It was predicted that the shock wave produced by each discharge could damage nearby gas-containing gut through a mechanism known to be associated with underwater detonations. Effects associated with rapid oscillation of the vapour bubble produced could cause damage to the wall of the bladder. Investigation of lithoclast discharges in vivo on goat, and in vitro on sheep bladders demonstrated perforation of both. In vitro studies in the rabbit demonstrated the predicted greater susceptibility of air-filled gut over that filled with fluid. These hazards would be even greater in the ureter and caution in the use of electronic fragmentation of vesical and ureteric calculi is urged in the light of these findings.", "contents": "Hazards to bladder and intestinal tissues from intravesical underwater electrical discharges from a surgical electronic lithoclast. Previous studies have shown that electronic lithoclast discharges produce effects resembling an underwater explosion. It was predicted that the shock wave produced by each discharge could damage nearby gas-containing gut through a mechanism known to be associated with underwater detonations. Effects associated with rapid oscillation of the vapour bubble produced could cause damage to the wall of the bladder. Investigation of lithoclast discharges in vivo on goat, and in vitro on sheep bladders demonstrated perforation of both. In vitro studies in the rabbit demonstrated the predicted greater susceptibility of air-filled gut over that filled with fluid. These hazards would be even greater in the ureter and caution in the use of electronic fragmentation of vesical and ureteric calculi is urged in the light of these findings.", "PMID": 960320}
{"id": "pubmed23n0032_3867", "title": "Denudation of the urinary bladder mucosa in the cat by formaldehyde.", "content": "Chemical Stripping of the urinary bladder mucosa was studied in 38 cats using 5 to 25% formaldehyde solutions. The contact time varied from 1 to 20 min. With a 20% solution and contact time of 1 min, total denudation was possible without necrosis of subepithelial layers. In such cases, complete reepithelialisation and normal bladder dynamics were seen within 3-4 weeks after formaldehyde instillation. Signs of formaldehyde intoxication due to vesical resorption were not observed.", "contents": "Denudation of the urinary bladder mucosa in the cat by formaldehyde. Chemical Stripping of the urinary bladder mucosa was studied in 38 cats using 5 to 25% formaldehyde solutions. The contact time varied from 1 to 20 min. With a 20% solution and contact time of 1 min, total denudation was possible without necrosis of subepithelial layers. In such cases, complete reepithelialisation and normal bladder dynamics were seen within 3-4 weeks after formaldehyde instillation. Signs of formaldehyde intoxication due to vesical resorption were not observed.", "PMID": 960321}
{"id": "pubmed23n0032_3868", "title": "A morphological study of the prostatic lobes and the seminal vesicles of castrated rats injected with oestradiol and/or insulin.", "content": "The growth of the ventral and dorsolateral prostate, the coagulating glands and the seminal vesicles was studied morphologically in castrated rats following 21 daily injections of oestradiol benzoate or protamine zinc insulin alone or in combination. Oestradiol benzoate was given in daily doses of 0.010 mg, protamine zinc insulin in daily doses of 10 IU. In the ventral and dorsolateral prostate oestradiol had an androgen-like action inducing epithelial growth and secretory activity in the epithelium. In the seminal vesicles and coagulating glands oestradiol induced an increased fibromuscular growth. Protamine zinc insulin induced growth and secretory activity in the dorsolateral prostate, while no such effect was observed in the other accessory reproductive glands. Protamine zinc insulin accentuated the androgen-like action of oestradiol benzoate on the epithelium of the dorsolateral prostate but did not influence on the increased fibromuscular growth seen after oestradiol in the coagulating glands and the seminal vesicles.", "contents": "A morphological study of the prostatic lobes and the seminal vesicles of castrated rats injected with oestradiol and/or insulin. The growth of the ventral and dorsolateral prostate, the coagulating glands and the seminal vesicles was studied morphologically in castrated rats following 21 daily injections of oestradiol benzoate or protamine zinc insulin alone or in combination. Oestradiol benzoate was given in daily doses of 0.010 mg, protamine zinc insulin in daily doses of 10 IU. In the ventral and dorsolateral prostate oestradiol had an androgen-like action inducing epithelial growth and secretory activity in the epithelium. In the seminal vesicles and coagulating glands oestradiol induced an increased fibromuscular growth. Protamine zinc insulin induced growth and secretory activity in the dorsolateral prostate, while no such effect was observed in the other accessory reproductive glands. Protamine zinc insulin accentuated the androgen-like action of oestradiol benzoate on the epithelium of the dorsolateral prostate but did not influence on the increased fibromuscular growth seen after oestradiol in the coagulating glands and the seminal vesicles.", "PMID": 960322}
{"id": "pubmed23n0032_3869", "title": "[New aspects in the treatment of oxalate lithiasis (author's transl)].", "content": "The causes of hypercalciuria and simple diagnostic criteria for the various forms of hypercalciuria are outlined. Indications, effectiveness, limitations, and side effects of cellulose phosphate are described. Emphasis is placed on the biochemical pathogenesis and classification of hyperoxaluria. The problems of measuring and controlling oxalate excretion in patients with hyperoxaluria and calcium oxalate stones are discussed. Succinimide offers a partly successful approach to the reduction of endogenous oxalate synthesis.", "contents": "[New aspects in the treatment of oxalate lithiasis (author's transl)]. The causes of hypercalciuria and simple diagnostic criteria for the various forms of hypercalciuria are outlined. Indications, effectiveness, limitations, and side effects of cellulose phosphate are described. Emphasis is placed on the biochemical pathogenesis and classification of hyperoxaluria. The problems of measuring and controlling oxalate excretion in patients with hyperoxaluria and calcium oxalate stones are discussed. Succinimide offers a partly successful approach to the reduction of endogenous oxalate synthesis.", "PMID": 960323}
{"id": "pubmed23n0032_3870", "title": "[Calcium and magnesium concentrations in \"Healthy\" and lithiasic human kidney (author's transl)].", "content": "Calcium and magnesium levels in the cortex, medulla, and papilla of human kidney from 32 so-called healthy patients and from eleven patients with calcium-oxalate lithiasis were determined by atom-absorption spectralphotometry. A positive calcium gradient with the highest calcium concentration in the papilla was found in all kidneys. Compared to the control group, that calcium concentration in the lithiasic kidneys was reduced by 50% in the papilla, but in the cortex and medulla, the levels were the same. A relative depletion of calcium in the papilla in hypercalciuria goes against the theory that the papilla is the main center of development of calcium-containing stones. The magnesium concentration was practically the same in cortex, medulla, and papilla, and no significant difference was found between lithiasic and healthy kidneys. These findings underline the central role of calcium in the genesis of calcium-containing stones.", "contents": "[Calcium and magnesium concentrations in \"Healthy\" and lithiasic human kidney (author's transl)]. Calcium and magnesium levels in the cortex, medulla, and papilla of human kidney from 32 so-called healthy patients and from eleven patients with calcium-oxalate lithiasis were determined by atom-absorption spectralphotometry. A positive calcium gradient with the highest calcium concentration in the papilla was found in all kidneys. Compared to the control group, that calcium concentration in the lithiasic kidneys was reduced by 50% in the papilla, but in the cortex and medulla, the levels were the same. A relative depletion of calcium in the papilla in hypercalciuria goes against the theory that the papilla is the main center of development of calcium-containing stones. The magnesium concentration was practically the same in cortex, medulla, and papilla, and no significant difference was found between lithiasic and healthy kidneys. These findings underline the central role of calcium in the genesis of calcium-containing stones.", "PMID": 960324}
{"id": "pubmed23n0032_3871", "title": "[Intraoperative use of an image intensifier in coral calculus operations (author's transl)].", "content": "The applicability of the image converter in kidneys stone operations, and particularly in coral calculus operations, is discussed. A comparison is made with other X-ray methods and the advantages of an extended area of use of the image converter is presented.", "contents": "[Intraoperative use of an image intensifier in coral calculus operations (author's transl)]. The applicability of the image converter in kidneys stone operations, and particularly in coral calculus operations, is discussed. A comparison is made with other X-ray methods and the advantages of an extended area of use of the image converter is presented.", "PMID": 960325}
{"id": "pubmed23n0032_3872", "title": "[The bladder-stone punch--a new principle of visual lithotripsy (author's transl)].", "content": "Description of a bladder-stone lithotriptor which works similarly to a punch resectoscope. This 24 French instrument can be used in simultaneously with electrohydraulic lithotripsy (Urat I), i.e., stones of the size of a cherry can be simply punched, while all larger stones may be destroyed first by electrohydraulic lithotripsy and then cut into smaller pieces for removal with the punch. The instrument, with irrigation qualities and a sight like a resectoscope, allows a quick operation, so that lithotripsy and TUR of the prostate may be performed opportunely.", "contents": "[The bladder-stone punch--a new principle of visual lithotripsy (author's transl)]. Description of a bladder-stone lithotriptor which works similarly to a punch resectoscope. This 24 French instrument can be used in simultaneously with electrohydraulic lithotripsy (Urat I), i.e., stones of the size of a cherry can be simply punched, while all larger stones may be destroyed first by electrohydraulic lithotripsy and then cut into smaller pieces for removal with the punch. The instrument, with irrigation qualities and a sight like a resectoscope, allows a quick operation, so that lithotripsy and TUR of the prostate may be performed opportunely.", "PMID": 960326}
{"id": "pubmed23n0032_3873", "title": "[Electrial aspects of transurethral resection with high-frequency currents (author's transl)].", "content": "With the help of electrical models the current-density distributions within the human body during transurethral resection have been measured for systems with various electrodes. The results lead to rules for avoiding mistakes.", "contents": "[Electrial aspects of transurethral resection with high-frequency currents (author's transl)]. With the help of electrical models the current-density distributions within the human body during transurethral resection have been measured for systems with various electrodes. The results lead to rules for avoiding mistakes.", "PMID": 960327}
{"id": "pubmed23n0032_3874", "title": "[Gastrointestinal fistulae of the upper urinary tract (author's transl)].", "content": "A case of ureteroduodenal fistula is presented. The subject of gastrointestinal fistulae of the upper urinary tract is reviewed. These fistulae usually exhibit a sparse atypical symptomatology in contrast to fistulae of the lower urinary tract. Retrograde ureteropyelography proves to be the most helpful diagnostic tool. Removal of the involved kidney has been found to be necessary in most of the published cases.", "contents": "[Gastrointestinal fistulae of the upper urinary tract (author's transl)]. A case of ureteroduodenal fistula is presented. The subject of gastrointestinal fistulae of the upper urinary tract is reviewed. These fistulae usually exhibit a sparse atypical symptomatology in contrast to fistulae of the lower urinary tract. Retrograde ureteropyelography proves to be the most helpful diagnostic tool. Removal of the involved kidney has been found to be necessary in most of the published cases.", "PMID": 960328}
{"id": "pubmed23n0032_3875", "title": "[Development of an implantable artificial urinary sphincter (author's transl)].", "content": "Fifteen years experience with investigations of the neurologic aspects of lower urinary tract function has produced a therapeutic rationale that includes utilization of prosthetic devices in selected patients. Development of an implantable artificial sphincter evolved as a necessity for restoring urinary continence became apparent. The experimental design of animal models and the technologic methods in device development are herein described.", "contents": "[Development of an implantable artificial urinary sphincter (author's transl)]. Fifteen years experience with investigations of the neurologic aspects of lower urinary tract function has produced a therapeutic rationale that includes utilization of prosthetic devices in selected patients. Development of an implantable artificial sphincter evolved as a necessity for restoring urinary continence became apparent. The experimental design of animal models and the technologic methods in device development are herein described.", "PMID": 960329}
{"id": "pubmed23n0032_3876", "title": "[Inverted urothelial papilloma (author's transl)].", "content": "Casuistic report dealing with the observation of an inverted papilloma arising from the pars prostatica urethrae. By means of the literature, clinical and pathologic problems of this scarcely known neoplasm, which was not delimitated in the German literature before 1963, are discussed.", "contents": "[Inverted urothelial papilloma (author's transl)]. Casuistic report dealing with the observation of an inverted papilloma arising from the pars prostatica urethrae. By means of the literature, clinical and pathologic problems of this scarcely known neoplasm, which was not delimitated in the German literature before 1963, are discussed.", "PMID": 960330}
{"id": "pubmed23n0032_3877", "title": "[On the size of the male urethra. Consequences of transurethral prostatic resection (author's transl)].", "content": "More recently it has been claimed by some authors that a disproportion between the size of the resectoscope and the lumen of the urethra is supposedly the cause of urethral strictures following transurethral prostatic resection. For this reason the use of resectoscopes no larger than 24 F in size has been urged. Reports in the literature as well as our own investigations in connection with the transurethral resection by cold punch technique cast some doubt on the wisdom of this statement as long as other causes, such as thermal damage during electroresection, are not definitely excluded.", "contents": "[On the size of the male urethra. Consequences of transurethral prostatic resection (author's transl)]. More recently it has been claimed by some authors that a disproportion between the size of the resectoscope and the lumen of the urethra is supposedly the cause of urethral strictures following transurethral prostatic resection. For this reason the use of resectoscopes no larger than 24 F in size has been urged. Reports in the literature as well as our own investigations in connection with the transurethral resection by cold punch technique cast some doubt on the wisdom of this statement as long as other causes, such as thermal damage during electroresection, are not definitely excluded.", "PMID": 960331}
{"id": "pubmed23n0032_3878", "title": "[Transurethral resection in patients with cardiac pacemakers (author's transl)].", "content": "Problems of transurethral resection in patients with cardiac pacemakers are discussed. Various complications caused by interference of cutting and coagulating currents with the pacemaker system and their possible prevention are pointed out. It is concluded that transurethral resection by cold punch technique has significant advantages over electroresection in patients with a cardiac pacemaker.", "contents": "[Transurethral resection in patients with cardiac pacemakers (author's transl)]. Problems of transurethral resection in patients with cardiac pacemakers are discussed. Various complications caused by interference of cutting and coagulating currents with the pacemaker system and their possible prevention are pointed out. It is concluded that transurethral resection by cold punch technique has significant advantages over electroresection in patients with a cardiac pacemaker.", "PMID": 960332}
{"id": "pubmed23n0032_3879", "title": "[Influence of anthraquinones on the formation of urinary calculi in experimental animals (author's transl)].", "content": "The growth-inhibiting influence of hydroxy-anthraquinone derivatives of root of rubia in various calcium offerings was investigated using a foreign-body bladder calculus model in rabbits. Following oral doses of glycoside-bound and free aglycemics a pronounced calcium-complex binding effect and a significant reduction in the growth rate of the calculi was observed. Use of anthraquinone glycosides to prevent recurrence of calcium-containing urinary stones is recommended.", "contents": "[Influence of anthraquinones on the formation of urinary calculi in experimental animals (author's transl)]. The growth-inhibiting influence of hydroxy-anthraquinone derivatives of root of rubia in various calcium offerings was investigated using a foreign-body bladder calculus model in rabbits. Following oral doses of glycoside-bound and free aglycemics a pronounced calcium-complex binding effect and a significant reduction in the growth rate of the calculi was observed. Use of anthraquinone glycosides to prevent recurrence of calcium-containing urinary stones is recommended.", "PMID": 960333}
{"id": "pubmed23n0032_3880", "title": "On the structure and importance of the epithelium in the human ductus epididymidis (author's transl).", "content": "In the wall of the ductus epididymidis rosette-like crystalloids and in the epithelium three types of cells are to be found: round basal cells, pale cylindrical epithelium and dark cylindrical epithelium. The latter shows a high metabolic activity with lamellar rough ergastoplasm and low vesicular secretion. Apocrine separation of drops of secretion is seen only sporadically. The functional relations between these states, the possibilities of biosynthesis in the epididymis, and the maturing of spermatozoa are discussed.", "contents": "On the structure and importance of the epithelium in the human ductus epididymidis (author's transl). In the wall of the ductus epididymidis rosette-like crystalloids and in the epithelium three types of cells are to be found: round basal cells, pale cylindrical epithelium and dark cylindrical epithelium. The latter shows a high metabolic activity with lamellar rough ergastoplasm and low vesicular secretion. Apocrine separation of drops of secretion is seen only sporadically. The functional relations between these states, the possibilities of biosynthesis in the epididymis, and the maturing of spermatozoa are discussed.", "PMID": 960334}
{"id": "pubmed23n0032_3881", "title": "Renal arteriovenous fistulas.", "content": "A review of the literature reveals that aggressive treatment of symptomatic intrarenal arteriovenous fistulas, whether by surgical or radiologic techniques, is generally indicated. The exception is those fistulas which result from closed renal biopsy since these usually heal spontaneously. The ultimate goal of any form of therapy is the maximal preservation of renal tissue. Methods of treatment are discussed, and a review of classification, etiology, incidence, symptoms, physiologic consequences, and means of diagnosis is given. A case of post-traumatic intrarenal arteriovenous fistula with ipsilaterally elevated renal vein renin and hypertension, cured by partial nephrectomy, is presented.", "contents": "Renal arteriovenous fistulas. A review of the literature reveals that aggressive treatment of symptomatic intrarenal arteriovenous fistulas, whether by surgical or radiologic techniques, is generally indicated. The exception is those fistulas which result from closed renal biopsy since these usually heal spontaneously. The ultimate goal of any form of therapy is the maximal preservation of renal tissue. Methods of treatment are discussed, and a review of classification, etiology, incidence, symptoms, physiologic consequences, and means of diagnosis is given. A case of post-traumatic intrarenal arteriovenous fistula with ipsilaterally elevated renal vein renin and hypertension, cured by partial nephrectomy, is presented.", "PMID": 960335}
{"id": "pubmed23n0032_3882", "title": "Control of renal arterial bleeding after percutaneous biopsy.", "content": "Transcatheter arterial embolization is becoming an accepted method of controlling massive acute hemorrhage, including that secondary to blunt and penetrating renal trauma. Two patients with retroperitoneal bleeding postrenal biopsy and angiographically proved pseudoaneurysms at the biopsy sites had successful embolization without sequelae. Experimental and clinical experience with transcatheter embolization is reviewed. It appears that this modality is a safe, effective way of controlling renal hemorrhage.", "contents": "Control of renal arterial bleeding after percutaneous biopsy. Transcatheter arterial embolization is becoming an accepted method of controlling massive acute hemorrhage, including that secondary to blunt and penetrating renal trauma. Two patients with retroperitoneal bleeding postrenal biopsy and angiographically proved pseudoaneurysms at the biopsy sites had successful embolization without sequelae. Experimental and clinical experience with transcatheter embolization is reviewed. It appears that this modality is a safe, effective way of controlling renal hemorrhage.", "PMID": 960336}
{"id": "pubmed23n0032_3883", "title": "Medical management of refractory hematuria in sickle-cell trait.", "content": "Hematuria secondary to sickle-cell trait has on occasion proved refractory to medical management. We have adopted a new six-drug regimen to deal with this difficult problem. Herein is reported our experience with this protocol in 2 patients. Our results have been excellent, with hematuria clearing within twenty-four hours. The drug regimen is used in an attempt to alter the conditions that are favorable for sickling in the renal medulla.", "contents": "Medical management of refractory hematuria in sickle-cell trait. Hematuria secondary to sickle-cell trait has on occasion proved refractory to medical management. We have adopted a new six-drug regimen to deal with this difficult problem. Herein is reported our experience with this protocol in 2 patients. Our results have been excellent, with hematuria clearing within twenty-four hours. The drug regimen is used in an attempt to alter the conditions that are favorable for sickling in the renal medulla.", "PMID": 960337}
{"id": "pubmed23n0032_3884", "title": "Nonoliguric renal failure after transurethral prostatic resection.", "content": "Six cases of acute nonoliguric renal failure following transurethral prostatectomy were studied. This condition usually has a milder clinical course than the oliguric type; but some patients will need dialysis and fatalities can occur. Although it is relatively rare, every urologist shoud be aware of the entity. The first clue is usually increasing azotemia despite a normal or increased urine output.", "contents": "Nonoliguric renal failure after transurethral prostatic resection. Six cases of acute nonoliguric renal failure following transurethral prostatectomy were studied. This condition usually has a milder clinical course than the oliguric type; but some patients will need dialysis and fatalities can occur. Although it is relatively rare, every urologist shoud be aware of the entity. The first clue is usually increasing azotemia despite a normal or increased urine output.", "PMID": 960338}
{"id": "pubmed23n0032_3885", "title": "Clinical significance of serum acid phosphatase levels in advanced prostatic carcinoma.", "content": "This cooperative study was sponsored by the National Prostatic Cancer Project to determine the usefulness of serum acid phosphatase levels as a predictive indicator with regard to performance status, sites of metastases, response to treatment, and survival in patients with advanced prostatic carcinoma. The results indicate that survival was significantly shorter for those patients who had elevation of thier on-study (pretreatment) total serum acid phosphatase ler cent reduction of primary tumor mass, relief of pain, and acid phosphatase activity. No correlation could be demonstrated between serum acid phosphatase and performance status, site of metastases, and other criteria of response to therapy. It is concluded that this test as currently determined spectrophotometrically at this stage of disease and if employed alone is not sufficient to allow for total evaluation of the response of therapy. It is, however, helpful when used in correlation with the previously mentioned positive factors.", "contents": "Clinical significance of serum acid phosphatase levels in advanced prostatic carcinoma. This cooperative study was sponsored by the National Prostatic Cancer Project to determine the usefulness of serum acid phosphatase levels as a predictive indicator with regard to performance status, sites of metastases, response to treatment, and survival in patients with advanced prostatic carcinoma. The results indicate that survival was significantly shorter for those patients who had elevation of thier on-study (pretreatment) total serum acid phosphatase ler cent reduction of primary tumor mass, relief of pain, and acid phosphatase activity. No correlation could be demonstrated between serum acid phosphatase and performance status, site of metastases, and other criteria of response to therapy. It is concluded that this test as currently determined spectrophotometrically at this stage of disease and if employed alone is not sufficient to allow for total evaluation of the response of therapy. It is, however, helpful when used in correlation with the previously mentioned positive factors.", "PMID": 960339}
{"id": "pubmed23n0032_3886", "title": "Urinary amino acid excretion patterns in cancer of prostate.", "content": "The excretion of urinary amino acids obtained from acid hydrolysates were studied by gas chromatographic analysis in 16 normal male subjects and 48 patients with cancer of the prostate. There were no significant differences of the most commonly excreted amimo acids in normal or patient groups or between patients with different stages of the disease because of the large variances of excretion within groups as a whole. However, with few exceptions, the patient group excreted isoleucine whereas isoleucine excretion was not detected in the normal subjects under the same experimental conditions. These findings suggest that measurement of the urinary  excretion of isoleucine may be a useful parameter for the early detection of prostatic cancer.", "contents": "Urinary amino acid excretion patterns in cancer of prostate. The excretion of urinary amino acids obtained from acid hydrolysates were studied by gas chromatographic analysis in 16 normal male subjects and 48 patients with cancer of the prostate. There were no significant differences of the most commonly excreted amimo acids in normal or patient groups or between patients with different stages of the disease because of the large variances of excretion within groups as a whole. However, with few exceptions, the patient group excreted isoleucine whereas isoleucine excretion was not detected in the normal subjects under the same experimental conditions. These findings suggest that measurement of the urinary  excretion of isoleucine may be a useful parameter for the early detection of prostatic cancer.", "PMID": 960340}
{"id": "pubmed23n0032_3887", "title": "Prostatic tissue and fluid concentrations of trimethoprim and sulfamethoxazole: experimental and clinical studies.", "content": "The concentrations of trimethoprim and sulfamethoxazole were determined in prostatic fluid and tissue of the dog after constant intravenous infusion. Concentrations of trimethoprim in both prostatic fluid and tissue exceeded the simultaneous serum concentrations, whereas the sulfamethoxazole prostatic fluid and tissue concentrations were much lower than the simultaneous serum concentrations. Prostatic tissue obtained from patients undergoing transurethral resection of the prostate also contained trimethoprim in higher but sulfamethoxazole in lower concentrations than the simultaneous serum concentrations after oral administration of these drugs preoperatively. In a patient with urinary diversion, trimethoprim was found in prostatic fluid in concentrations as high as forty-four times the simultaneous serum concentrations whereas sulfamethoxazole was found in concentrations representing only a fraction of the simultaneous serum concentrations. These findings lend support to the use of trimethoprim in the treatment of bacterial prostatitis.", "contents": "Prostatic tissue and fluid concentrations of trimethoprim and sulfamethoxazole: experimental and clinical studies. The concentrations of trimethoprim and sulfamethoxazole were determined in prostatic fluid and tissue of the dog after constant intravenous infusion. Concentrations of trimethoprim in both prostatic fluid and tissue exceeded the simultaneous serum concentrations, whereas the sulfamethoxazole prostatic fluid and tissue concentrations were much lower than the simultaneous serum concentrations. Prostatic tissue obtained from patients undergoing transurethral resection of the prostate also contained trimethoprim in higher but sulfamethoxazole in lower concentrations than the simultaneous serum concentrations after oral administration of these drugs preoperatively. In a patient with urinary diversion, trimethoprim was found in prostatic fluid in concentrations as high as forty-four times the simultaneous serum concentrations whereas sulfamethoxazole was found in concentrations representing only a fraction of the simultaneous serum concentrations. These findings lend support to the use of trimethoprim in the treatment of bacterial prostatitis.", "PMID": 960341}
{"id": "pubmed23n0032_3888", "title": "Use of dantrolene sodium in external sphincter spasm.", "content": "In various neurologic and non-neurologic diseases inappropriate hypertonicity of the external sphincter during micturition may cause outlet obstruction. Heretofore therapy for these disorders has been surgical: pudenal neurectomy or external sphincterotomy. These procedures are not without complication (incontinence, impotence, hemorrhage). A medical approach to this disorder is therefore warranted. In a selected group of patients with external sphincter spasm diagnosed by concitant sphincter electromyography, cystometrography, and voiding cystourethrography, dantrolene sodium (Dantrium) therapy was instituted. Follow-up studies have shown this drug to be effective in treating this disorder.", "contents": "Use of dantrolene sodium in external sphincter spasm. In various neurologic and non-neurologic diseases inappropriate hypertonicity of the external sphincter during micturition may cause outlet obstruction. Heretofore therapy for these disorders has been surgical: pudenal neurectomy or external sphincterotomy. These procedures are not without complication (incontinence, impotence, hemorrhage). A medical approach to this disorder is therefore warranted. In a selected group of patients with external sphincter spasm diagnosed by concitant sphincter electromyography, cystometrography, and voiding cystourethrography, dantrolene sodium (Dantrium) therapy was instituted. Follow-up studies have shown this drug to be effective in treating this disorder.", "PMID": 960342}
{"id": "pubmed23n0032_3889", "title": "Management of priapism by: cavernoglandular shunt.", "content": "Cavernoglandular shunt is a new simple operation consisting of the formation of a window between the corpus cavernosum and glans penis to establish drainage of trapped blood from the corpora cavernosum.", "contents": "Management of priapism by: cavernoglandular shunt. Cavernoglandular shunt is a new simple operation consisting of the formation of a window between the corpus cavernosum and glans penis to establish drainage of trapped blood from the corpora cavernosum.", "PMID": 960343}
{"id": "pubmed23n0032_3890", "title": "Hypospadias: results of surgical treatment and perspectives in management.", "content": "An institutional experience with the repair of hypospadias over a fifteen-year period is reviewed. The 119 patients treated surigcally serve as the substance of this report. Recommendations concerning options in surgical treatment are predicated on this experience and the contemporary literature.", "contents": "Hypospadias: results of surgical treatment and perspectives in management. An institutional experience with the repair of hypospadias over a fifteen-year period is reviewed. The 119 patients treated surigcally serve as the substance of this report. Recommendations concerning options in surgical treatment are predicated on this experience and the contemporary literature.", "PMID": 960344}
{"id": "pubmed23n0032_3891", "title": "Malignancy associated with horseshoe kidney.", "content": "A compilation of the reported cases of cancer in horseshoe kidney from 1895 to 1975 is presented along with a comparison of the incidence of the various types of tumor involved from 1895 to 1966 and 1966 to 1975. An additional case of hypernephroma is added to the literature for a total of 111 cases reported to date. The increase incidence of both carcinoma of the renal pelvis and nephroblastoma is discussed.", "contents": "Malignancy associated with horseshoe kidney. A compilation of the reported cases of cancer in horseshoe kidney from 1895 to 1975 is presented along with a comparison of the incidence of the various types of tumor involved from 1895 to 1966 and 1966 to 1975. An additional case of hypernephroma is added to the literature for a total of 111 cases reported to date. The increase incidence of both carcinoma of the renal pelvis and nephroblastoma is discussed.", "PMID": 960345}
{"id": "pubmed23n0032_3892", "title": "Traumatic rupture of horseshoe kidney with partial ureteral duplication associated with supernumerary kidney.", "content": "The first reported case of traumatic rupture of a horseshoe kidney with partial ureteral duplication and an associated supernumerary kidney is presented. A review of the incidence, hypothesis of embryogenesis, associated anomalous development, and acute surgical management of this unique renal anomaly is provided.", "contents": "Traumatic rupture of horseshoe kidney with partial ureteral duplication associated with supernumerary kidney. The first reported case of traumatic rupture of a horseshoe kidney with partial ureteral duplication and an associated supernumerary kidney is presented. A review of the incidence, hypothesis of embryogenesis, associated anomalous development, and acute surgical management of this unique renal anomaly is provided.", "PMID": 960346}
{"id": "pubmed23n0032_3893", "title": "Congenital varicella syndrome with genitourinary anomalies.", "content": "A female child, born at term to a mother who contracted varicella in early pregnancy, presented with multiple congenital defects. These included mental retardation, numerous skeletal anomalies, and absent uterus and vagina. Urologic anomalies included bilateral chronic pyelonephritis secondary to vesicoureteric reflux. This pattern of congenital abnormalities has not been reported previously.", "contents": "Congenital varicella syndrome with genitourinary anomalies. A female child, born at term to a mother who contracted varicella in early pregnancy, presented with multiple congenital defects. These included mental retardation, numerous skeletal anomalies, and absent uterus and vagina. Urologic anomalies included bilateral chronic pyelonephritis secondary to vesicoureteric reflux. This pattern of congenital abnormalities has not been reported previously.", "PMID": 960347}
{"id": "pubmed23n0032_3894", "title": "Bilateral successive testicular cancer of different cell types.", "content": "The fourth case of bilateral successive testicular tumors of different cell types is reported (seminoma and embryonal carcinoma), and the English literature on bilateral testicular tumors from 1965 to 1975 is reviewed. The absence of direct organ extension or demonstrable metastatic lymphatic or vascular involvement points to the primary origin of these lesions.", "contents": "Bilateral successive testicular cancer of different cell types. The fourth case of bilateral successive testicular tumors of different cell types is reported (seminoma and embryonal carcinoma), and the English literature on bilateral testicular tumors from 1965 to 1975 is reviewed. The absence of direct organ extension or demonstrable metastatic lymphatic or vascular involvement points to the primary origin of these lesions.", "PMID": 960348}
{"id": "pubmed23n0032_3895", "title": "Endometrial carcinoma of prostatic utricle with osseous metastases.", "content": "Endometrial carcinoma of the prostatic utricle is a rare pupillary prostatic tumor arising in the region of the prostatic urethra and verumontanum. Since the first description by Melicow and Pachter in 1967, 8 additional cases have been reported. This case is the tenth reported, and the first in which metastases to bone have been proved by biopsy. Transurethral resection of the tumor with postoperative radiation therapy to the prostatic region and metastases has resulted in clinical improvement. The importance of accurate diagnosis of this neoplasm is emphasized to avoid unnecessary delay in the appropriate choice of therapeutic modalities.", "contents": "Endometrial carcinoma of prostatic utricle with osseous metastases. Endometrial carcinoma of the prostatic utricle is a rare pupillary prostatic tumor arising in the region of the prostatic urethra and verumontanum. Since the first description by Melicow and Pachter in 1967, 8 additional cases have been reported. This case is the tenth reported, and the first in which metastases to bone have been proved by biopsy. Transurethral resection of the tumor with postoperative radiation therapy to the prostatic region and metastases has resulted in clinical improvement. The importance of accurate diagnosis of this neoplasm is emphasized to avoid unnecessary delay in the appropriate choice of therapeutic modalities.", "PMID": 960351}
{"id": "pubmed23n0032_3896", "title": "Benign fibrous paratesticular tumors.", "content": "Utilizing the criteria of Goodwin, a case each of chronic proliferative periorchitis and multiple fibromas of the tunica vaginalis testis causing paratesticular tumors is presented and discussed. Both of these rate processes appear to be distinct and separate clinical entities, although they appear to be similar histologically. With increased awareness by those treating intrascrotal masses, these processes may be correctly recognized and more testes may be salvaged.", "contents": "Benign fibrous paratesticular tumors. Utilizing the criteria of Goodwin, a case each of chronic proliferative periorchitis and multiple fibromas of the tunica vaginalis testis causing paratesticular tumors is presented and discussed. Both of these rate processes appear to be distinct and separate clinical entities, although they appear to be similar histologically. With increased awareness by those treating intrascrotal masses, these processes may be correctly recognized and more testes may be salvaged.", "PMID": 960352}
{"id": "pubmed23n0032_3897", "title": "Coexisting avascular lesions and renal cell carcinoma.", "content": "An analysis of 99 cases of coexisting renal avascular lesions and renal cell carcinoma suggests that: (1) a direct pathogenetic relationship exists in the majority; (2) the cyst-related carcinoma tends to occur at an earlier age; (3) angiography alone is not reliable for the diagnosis of an apparently benign renal mass, and (4) individuals with polycystic kidneys share at least an equal risk of renal carcinoma as the general population.", "contents": "Coexisting avascular lesions and renal cell carcinoma. An analysis of 99 cases of coexisting renal avascular lesions and renal cell carcinoma suggests that: (1) a direct pathogenetic relationship exists in the majority; (2) the cyst-related carcinoma tends to occur at an earlier age; (3) angiography alone is not reliable for the diagnosis of an apparently benign renal mass, and (4) individuals with polycystic kidneys share at least an equal risk of renal carcinoma as the general population.", "PMID": 960354}
{"id": "pubmed23n0032_3898", "title": "Intrarenal pheochromocytoma: preoperative angiographic diagnosis.", "content": "This report documents the angiographic, urographic, and tomographic findings in a patients with a pheochromocytoma within the renal capsule and separate from a normal adrenal gland.", "contents": "Intrarenal pheochromocytoma: preoperative angiographic diagnosis. This report documents the angiographic, urographic, and tomographic findings in a patients with a pheochromocytoma within the renal capsule and separate from a normal adrenal gland.", "PMID": 960355}
{"id": "pubmed23n0032_3899", "title": "Recertification of professional competence. American Board of Ophthalmology.", "content": "Recognizing the steady expansion of medical knowledge, the societal desire to ensure the best possible medical care, and governmental concern for verification of physician competence, the American Board of Ophthalmology is preparing to offer a voluntary recertification program for Diplomates of the Board who wish to establish current professional competence. Pertinent to this program, consideration is given to the rationale for periodic recertification, relevant actions by representative specialty boards and the American Board of Medical Specialties, and fuidelines for development of a recertification process by the American Board of Ophthalmology. These guidelines are set forth to provide a basis for study and evaluation by all interested parties. By  thoughtful, broadly based, and coordinated consideration, policies will evolve to serve concurrently the best interest of the general public and of the ophthalmic profession.", "contents": "Recertification of professional competence. American Board of Ophthalmology. Recognizing the steady expansion of medical knowledge, the societal desire to ensure the best possible medical care, and governmental concern for verification of physician competence, the American Board of Ophthalmology is preparing to offer a voluntary recertification program for Diplomates of the Board who wish to establish current professional competence. Pertinent to this program, consideration is given to the rationale for periodic recertification, relevant actions by representative specialty boards and the American Board of Medical Specialties, and fuidelines for development of a recertification process by the American Board of Ophthalmology. These guidelines are set forth to provide a basis for study and evaluation by all interested parties. By  thoughtful, broadly based, and coordinated consideration, policies will evolve to serve concurrently the best interest of the general public and of the ophthalmic profession.", "PMID": 960356}
{"id": "pubmed23n0032_3900", "title": "Pars plana vitrectomy. Microsurgical pars plana lensectomy.", "content": "Pars plana lensectomy, wherein the entire lens within the pupillary area (the anterior capsule, anterior cortex, nucleus, posterior cortex, and posterior capsule) is removed, is now a well-established technique. It is to be considered as a method of choice for the removal of presenile cataracts in patients under the age of 40, developmental cataracts, cataracta complicata secondary to uveitis, subluxated and luxated cataracts. Pars plana lensectomy of the senile cataract is limited to a grade 2 nuclear sclerosis on a scale of 4. The use of ultrasonic instrumentation via the pars plana in conjunction with vitreous instrumentation permits the removal of the grade 3 nuclear sclerotic cataract. A pars plana two-instrument technique is used for the extraction of the totally luxated senile cataract no matter how sclerotic the nucleus may be.", "contents": "Pars plana vitrectomy. Microsurgical pars plana lensectomy. Pars plana lensectomy, wherein the entire lens within the pupillary area (the anterior capsule, anterior cortex, nucleus, posterior cortex, and posterior capsule) is removed, is now a well-established technique. It is to be considered as a method of choice for the removal of presenile cataracts in patients under the age of 40, developmental cataracts, cataracta complicata secondary to uveitis, subluxated and luxated cataracts. Pars plana lensectomy of the senile cataract is limited to a grade 2 nuclear sclerosis on a scale of 4. The use of ultrasonic instrumentation via the pars plana in conjunction with vitreous instrumentation permits the removal of the grade 3 nuclear sclerotic cataract. A pars plana two-instrument technique is used for the extraction of the totally luxated senile cataract no matter how sclerotic the nucleus may be.", "PMID": 960359}
{"id": "pubmed23n0032_3901", "title": "Pars plana vitrectomy. Vitrectomy treatment of vitreous opacities.", "content": "Twenty-one patients suffering from vitreous opacities caused by central- and branch-vein occlusion, sickle cell retinopathy, retinal vasculitis, endophthalmitis, hypertensive retinopathy, and uveitis underwent pars plana vitrectomy with the vitrophage. Intraoperative complications included retinal tears in two eyes with sickle cell retinopathy and tractional retinal detachment. Both were successfully treated. Postoperative complications were recurrent vitreous hemorrhage, hemolytic glaucoma, and corneal blood staining in a patient suffering from sickle cell retinopathy. Long-standing corneal edema was observed in patients suffering from endophthalmitis. Visual acuity improved in 95% of our patients and was worse in 5% of the patients.", "contents": "Pars plana vitrectomy. Vitrectomy treatment of vitreous opacities. Twenty-one patients suffering from vitreous opacities caused by central- and branch-vein occlusion, sickle cell retinopathy, retinal vasculitis, endophthalmitis, hypertensive retinopathy, and uveitis underwent pars plana vitrectomy with the vitrophage. Intraoperative complications included retinal tears in two eyes with sickle cell retinopathy and tractional retinal detachment. Both were successfully treated. Postoperative complications were recurrent vitreous hemorrhage, hemolytic glaucoma, and corneal blood staining in a patient suffering from sickle cell retinopathy. Long-standing corneal edema was observed in patients suffering from endophthalmitis. Visual acuity improved in 95% of our patients and was worse in 5% of the patients.", "PMID": 960361}
{"id": "pubmed23n0032_3902", "title": "Pars plana virectomy. Preoperative evaluation.", "content": "We have outlined the essential considerations in evaluating patients for closed vitrectomy and have attempted to show the importance of each. In general, we recommend closed virectomy under the following conditions: (1) vitreous opacities showing no resolution after six months of observation, (2) vision which is worse than 20/200 but has at least light perception with accurate projection, (3) there is no rubeosis iridis or glaucoma, (4) ultrasonography, ERG, and VER reveal a functional retina and optic nerve, and (5) the benefit outweighs the risk of surgery.", "contents": "Pars plana virectomy. Preoperative evaluation. We have outlined the essential considerations in evaluating patients for closed vitrectomy and have attempted to show the importance of each. In general, we recommend closed virectomy under the following conditions: (1) vitreous opacities showing no resolution after six months of observation, (2) vision which is worse than 20/200 but has at least light perception with accurate projection, (3) there is no rubeosis iridis or glaucoma, (4) ultrasonography, ERG, and VER reveal a functional retina and optic nerve, and (5) the benefit outweighs the risk of surgery.", "PMID": 960358}
{"id": "pubmed23n0032_3903", "title": "Pars plana vitrectomy. Vitrectomy in diabetic retinopathy.", "content": "In spite of the rather high rate of complications, it is clear that in selected cases of proliferative diabetic retinopathy with massive long-standing vitreous hemorrhage, significant improvement can be accomplished by pars plana vitrectomy. However, the number of eyes recovering 20/40 vision or better remains small (7% in our series at 9 to 15 months postvitrectomy), and consideration needs to be given to means to improve these figures. Whether vitrectomy at an earlier stage of the retinopathy can accomplish this is the subject of an upcoming cooperative study under the sponsorship of the National Eye Institute.", "contents": "Pars plana vitrectomy. Vitrectomy in diabetic retinopathy. In spite of the rather high rate of complications, it is clear that in selected cases of proliferative diabetic retinopathy with massive long-standing vitreous hemorrhage, significant improvement can be accomplished by pars plana vitrectomy. However, the number of eyes recovering 20/40 vision or better remains small (7% in our series at 9 to 15 months postvitrectomy), and consideration needs to be given to means to improve these figures. Whether vitrectomy at an earlier stage of the retinopathy can accomplish this is the subject of an upcoming cooperative study under the sponsorship of the National Eye Institute.", "PMID": 960362}
{"id": "pubmed23n0032_3904", "title": "Pars plana vitrectomy. Vitrectomy technique in anterior segment surgery.", "content": "Instrumentation and techniques developed primarily for vitreoretinal surgery were used to treat 20 eyes with an occluded or inadequate pupillary space and 5 aphakic eyes with complications caused by formed vitreous in the anterior chamber. A pars plana approach was used in 19 eyes and a limbus approach was used in 6 eyes. In every eye an adequate pupillary space was provided and all formed vitreous was removed from the anterior segment. No intraoperative complications occurred. This new approach to selected cases of  anterior segment surgery offers several advantages over conventional techniques. A larger number of cases and longer period of follow-up are necessary to further define the specific indications and limitations of this technique.", "contents": "Pars plana vitrectomy. Vitrectomy technique in anterior segment surgery. Instrumentation and techniques developed primarily for vitreoretinal surgery were used to treat 20 eyes with an occluded or inadequate pupillary space and 5 aphakic eyes with complications caused by formed vitreous in the anterior chamber. A pars plana approach was used in 19 eyes and a limbus approach was used in 6 eyes. In every eye an adequate pupillary space was provided and all formed vitreous was removed from the anterior segment. No intraoperative complications occurred. This new approach to selected cases of  anterior segment surgery offers several advantages over conventional techniques. A larger number of cases and longer period of follow-up are necessary to further define the specific indications and limitations of this technique.", "PMID": 960360}
{"id": "pubmed23n0032_3905", "title": "Pars plana vitrectomy. The role of vitrectomy in penetrating ocular injuries.", "content": "Pars plana vitrectomy and related procedures occupy an important role in the management of perforating injuries. Vitreous surgery is particularly important in rendering retinal detachments observable and operable. The frequent postvitrectomy complication of delayed retinal detachment appears to be reduced with prophylactic encircling scleral buckle procedures. Although certain categories of injuries continue to carry a poor prognosis, earlier surgical intervention promises improved results.", "contents": "Pars plana vitrectomy. The role of vitrectomy in penetrating ocular injuries. Pars plana vitrectomy and related procedures occupy an important role in the management of perforating injuries. Vitreous surgery is particularly important in rendering retinal detachments observable and operable. The frequent postvitrectomy complication of delayed retinal detachment appears to be reduced with prophylactic encircling scleral buckle procedures. Although certain categories of injuries continue to carry a poor prognosis, earlier surgical intervention promises improved results.", "PMID": 960365}
{"id": "pubmed23n0032_3906", "title": "Pars plana vitrectomy. The role of vitrectomy in traumatic vitreopathy.", "content": "Debridement of the vitreous from perforating injuries can be more effectively and expeditiously accomplished with the aid of vitreous suction and cutting instruments than with conventional sponge-forceps technique. This technique is of great value as part of primary closure of perforating ocular injuries. In addition, trauma which results in (1) lens rupture with expulsion of the lens material into the vitreous; (2) massive vitreous hemorrhage with ciliary body laceration or posterior globe laceration; (3) retinal detachment with vitreous hemorrhage; or (4) retained reactive intraocular foreign body, requires vitrectomy. Because of inflammatory and fibroplastic changes, the earliest possible surgical intervention is recommended in these situations. Removal of lens material, reactive foreign body, or traction membranes, and early repair of retinal detachment may all be facilitated by new vitrectomy instrumentation.", "contents": "Pars plana vitrectomy. The role of vitrectomy in traumatic vitreopathy. Debridement of the vitreous from perforating injuries can be more effectively and expeditiously accomplished with the aid of vitreous suction and cutting instruments than with conventional sponge-forceps technique. This technique is of great value as part of primary closure of perforating ocular injuries. In addition, trauma which results in (1) lens rupture with expulsion of the lens material into the vitreous; (2) massive vitreous hemorrhage with ciliary body laceration or posterior globe laceration; (3) retinal detachment with vitreous hemorrhage; or (4) retained reactive intraocular foreign body, requires vitrectomy. Because of inflammatory and fibroplastic changes, the earliest possible surgical intervention is recommended in these situations. Removal of lens material, reactive foreign body, or traction membranes, and early repair of retinal detachment may all be facilitated by new vitrectomy instrumentation.", "PMID": 960364}
{"id": "pubmed23n0032_3907", "title": "Pars plana vitrectomy. Removal of preretinal membranes.", "content": "Removal of preretinal membranes in pars plana vitrectomy is achieved with the two-instrument technique by using a hooked needle or vitreous scissors, in addition to the vitrectomy instrument. Preretinal membranes are found in proliferative diabetic retinopathy, perforating injuries, and in retinal detachments with massive periretinal proliferation. Technique and results have been described in the text.", "contents": "Pars plana vitrectomy. Removal of preretinal membranes. Removal of preretinal membranes in pars plana vitrectomy is achieved with the two-instrument technique by using a hooked needle or vitreous scissors, in addition to the vitrectomy instrument. Preretinal membranes are found in proliferative diabetic retinopathy, perforating injuries, and in retinal detachments with massive periretinal proliferation. Technique and results have been described in the text.", "PMID": 960366}
{"id": "pubmed23n0032_3908", "title": "Ultrasonic fragmentation for vitrectomy and associated surgical procedures.", "content": "Ultrasonic fragmentation (USF) can be used to fragment various tissues such as the lens, the vitreous, the iris, and the ciliary body, so that they may be aspirated through a 23-gauge canula. Since the technique was first described in 1973, it has been used in 148 eyes in a variety of conditions with encouraging results. In aphakic bullous keratopathy, the corneas and vision improved in 78% of the cases. Pupillary block was relieved in 100% of cases. Cystoid macular edema improved in 63%. Vitreous opacification from other than hemorrhage cleared in all cases, and vision improved in 71%. Massive vitreous hemorrhage was cleared in 86%, and vision improved in 58%. In massive vitreous hemorrhage and retinal detachment, the vitreous was cleared and the retina reattached in 80% of cases. In massive vitreous contraction, the vitreous was removed in all cases and the retina reattached in all (100%) of cases. In massive vitreous membranes and retinal detachment, the membranes were successfully removed in 100% and the retina reattached in 82%. Lens remnants in the vitreous were removed in 100% of cases. Secondary membranes were removed successfully in 100% of cases. Vitreous presentation in the anterior chamber, caused by a ruptured posterior capsule during extracapsular extraction or actual vitreous loss during intracapsular extraction, was treated successfully in 100% of cases. One case of medullo-epithelioma of the ciliary body was removed but recurred twice after ultrasonic fragmentation. Complications occurred in seven of 148 cases (4.8%) and consisted of five cases of retinal detachment (probably because of lens material reaction) and two cases of epithelial downgrowth in cases where the wound was not closed with sutures. Ultrasonic fragmentation appears to be a fairly simple procedure, performed with a machine with no moving parts, through two 1-mm incisions and using two 0.6-mm cannulas, with a minimum of complications and encouraging results. It should be tried before more radical procedures are recommended. A good anatomic result was obtained in 129 of the 148 consecutive cases (88%). The technique is described and the results reported.", "contents": "Ultrasonic fragmentation for vitrectomy and associated surgical procedures. Ultrasonic fragmentation (USF) can be used to fragment various tissues such as the lens, the vitreous, the iris, and the ciliary body, so that they may be aspirated through a 23-gauge canula. Since the technique was first described in 1973, it has been used in 148 eyes in a variety of conditions with encouraging results. In aphakic bullous keratopathy, the corneas and vision improved in 78% of the cases. Pupillary block was relieved in 100% of cases. Cystoid macular edema improved in 63%. Vitreous opacification from other than hemorrhage cleared in all cases, and vision improved in 71%. Massive vitreous hemorrhage was cleared in 86%, and vision improved in 58%. In massive vitreous hemorrhage and retinal detachment, the vitreous was cleared and the retina reattached in 80% of cases. In massive vitreous contraction, the vitreous was removed in all cases and the retina reattached in all (100%) of cases. In massive vitreous membranes and retinal detachment, the membranes were successfully removed in 100% and the retina reattached in 82%. Lens remnants in the vitreous were removed in 100% of cases. Secondary membranes were removed successfully in 100% of cases. Vitreous presentation in the anterior chamber, caused by a ruptured posterior capsule during extracapsular extraction or actual vitreous loss during intracapsular extraction, was treated successfully in 100% of cases. One case of medullo-epithelioma of the ciliary body was removed but recurred twice after ultrasonic fragmentation. Complications occurred in seven of 148 cases (4.8%) and consisted of five cases of retinal detachment (probably because of lens material reaction) and two cases of epithelial downgrowth in cases where the wound was not closed with sutures. Ultrasonic fragmentation appears to be a fairly simple procedure, performed with a machine with no moving parts, through two 1-mm incisions and using two 0.6-mm cannulas, with a minimum of complications and encouraging results. It should be tried before more radical procedures are recommended. A good anatomic result was obtained in 129 of the 148 consecutive cases (88%). The technique is described and the results reported.", "PMID": 960369}
{"id": "pubmed23n0032_3909", "title": "The natural history of senile retinoschisis.", "content": "The natural history of senile retionoschisis was studied by observing a series of 193 eyes of 108 nonreferred patients who had no history of significant ocular disease, while refraining from the use of any form of prophylactic intervention, for follow-up periods ranging from 2 to 11 years. One patient at the outset of the study had double layer holes, which had already led to a localized subclinical retinal detachment that had been left untreated, and did not progress during observation for more than two years. One patient developed a small asymptomatic nonprogressive retinal detachment from an outer layer hole. Four eyes showed posterior extension, but in only one was this serious. Nine eyes developed new retinal breaks, one in both layers, but none progressed in extent of retinoschisis or to retinal detachment. Of particular interest were seven eyes which showed unmistakable regression, the retinoschisis disappearing completely in six eyes. Based on reported cases, an estimated computation is made which places the probable risk of frank, progressive, symptomatic retinal detachment resulting from retinoschisis at about 0.07%. Four indications for treatment are given which are considered justified at the present state of our knowledge.", "contents": "The natural history of senile retinoschisis. The natural history of senile retionoschisis was studied by observing a series of 193 eyes of 108 nonreferred patients who had no history of significant ocular disease, while refraining from the use of any form of prophylactic intervention, for follow-up periods ranging from 2 to 11 years. One patient at the outset of the study had double layer holes, which had already led to a localized subclinical retinal detachment that had been left untreated, and did not progress during observation for more than two years. One patient developed a small asymptomatic nonprogressive retinal detachment from an outer layer hole. Four eyes showed posterior extension, but in only one was this serious. Nine eyes developed new retinal breaks, one in both layers, but none progressed in extent of retinoschisis or to retinal detachment. Of particular interest were seven eyes which showed unmistakable regression, the retinoschisis disappearing completely in six eyes. Based on reported cases, an estimated computation is made which places the probable risk of frank, progressive, symptomatic retinal detachment resulting from retinoschisis at about 0.07%. Four indications for treatment are given which are considered justified at the present state of our knowledge.", "PMID": 960371}
{"id": "pubmed23n0032_3910", "title": "Subretinal tracts in ophthalmomyiasis.", "content": "Three cases of presumed infestation of the human globe by the maggot of a true fly (order of Diptera) are presented. In each case, migration of the maggot through the subretinal space produced widespread ophthalmoscopic and fluorescein angiographic changes which are believed to be pathognomonic of subretinal ophthalmomyiasis. Despite extensive changes in the retinal pigmented epithelium, each of the three patients expressed few symptoms and manifested minimal visual deficit.", "contents": "Subretinal tracts in ophthalmomyiasis. Three cases of presumed infestation of the human globe by the maggot of a true fly (order of Diptera) are presented. In each case, migration of the maggot through the subretinal space produced widespread ophthalmoscopic and fluorescein angiographic changes which are believed to be pathognomonic of subretinal ophthalmomyiasis. Despite extensive changes in the retinal pigmented epithelium, each of the three patients expressed few symptoms and manifested minimal visual deficit.", "PMID": 960373}
{"id": "pubmed23n0032_3911", "title": "Round atrophic holes in lattice degeneration--an important cause of phakic retinal detachment.", "content": "Round atrophic holes in lattice degeneration are an important cause of phakic retinal detachment. Detachments due solely to round holes in lattice accounted for almost 2.8% of all retinal detachments treated at Wills Eye Hospital from January 1970 to August 1973. These detachments had the following important characteristics: 1. One of the patients were under the age of 30 years. 2. Over 75% of the patients had refractive errors more myopic than -3 D spherical equivalent. 3. Inferior detachments were slightly more common than superior detachments. When located inferiorly, there was a tendency for slow progression as indicated by the frequent presence of pigmented demarcation lines. 4. Surgical repair with standard scleral buckling techniques was successful in 98% of these detachments. Young, moderate to highly myopic patients with round holes in areas of lattice degeneration seem to have a greater risk of developing this type of detachment. Patients with the triad of youth, myopia, and round holes in lattice degeneration deserve close observation.", "contents": "Round atrophic holes in lattice degeneration--an important cause of phakic retinal detachment. Round atrophic holes in lattice degeneration are an important cause of phakic retinal detachment. Detachments due solely to round holes in lattice accounted for almost 2.8% of all retinal detachments treated at Wills Eye Hospital from January 1970 to August 1973. These detachments had the following important characteristics: 1. One of the patients were under the age of 30 years. 2. Over 75% of the patients had refractive errors more myopic than -3 D spherical equivalent. 3. Inferior detachments were slightly more common than superior detachments. When located inferiorly, there was a tendency for slow progression as indicated by the frequent presence of pigmented demarcation lines. 4. Surgical repair with standard scleral buckling techniques was successful in 98% of these detachments. Young, moderate to highly myopic patients with round holes in areas of lattice degeneration seem to have a greater risk of developing this type of detachment. Patients with the triad of youth, myopia, and round holes in lattice degeneration deserve close observation.", "PMID": 960375}
{"id": "pubmed23n0032_3912", "title": "Nuclear lacrimal scan vs dacryocystography.", "content": "The lacrimal drainage systems of 78 eyes were studied by technetium scanning at the University of Iowa and, of these, 25 were also subjected to conventional contrast dacryocystography. Twenty-six eyes were asymptomatic for epiphora and normal scans were recorded on all of them. All of the remaining 52 symptomatic eyes had an abnormal lacrimal scan with either a presac or a postsac blockage. In general, there was good correlation with dacryocystography, with the exception of three cases of functional block which had a normal dacryocystogram. Technetium scanning should serve as a valuable tool for diagnosing and localizing lacrimal obstruction.", "contents": "Nuclear lacrimal scan vs dacryocystography. The lacrimal drainage systems of 78 eyes were studied by technetium scanning at the University of Iowa and, of these, 25 were also subjected to conventional contrast dacryocystography. Twenty-six eyes were asymptomatic for epiphora and normal scans were recorded on all of them. All of the remaining 52 symptomatic eyes had an abnormal lacrimal scan with either a presac or a postsac blockage. In general, there was good correlation with dacryocystography, with the exception of three cases of functional block which had a normal dacryocystogram. Technetium scanning should serve as a valuable tool for diagnosing and localizing lacrimal obstruction.", "PMID": 960379}
{"id": "pubmed23n0032_3913", "title": "Socket reconstruction: what really happens.", "content": "Reconstruction of contracted sockets can be extremely difficult and may result in a number of complications. Adherence to certain contraindications in attempting socket reformation can avoid some of these problems. Complications of socket reconstruction as discussed previously are (1) infection and (2) recurrent socket contracture, manifested either by extrusion of the acrylic plate placed in the orbit or by eyelid retraction.", "contents": "Socket reconstruction: what really happens. Reconstruction of contracted sockets can be extremely difficult and may result in a number of complications. Adherence to certain contraindications in attempting socket reformation can avoid some of these problems. Complications of socket reconstruction as discussed previously are (1) infection and (2) recurrent socket contracture, manifested either by extrusion of the acrylic plate placed in the orbit or by eyelid retraction.", "PMID": 960380}
{"id": "pubmed23n0032_3914", "title": "Treatment of acute eyelid trauma to prevent late complications.", "content": "A checklist for preventing late complications of acute eyelid trauma should include recognizing injury to the globe, preventing infection, and achieving proper coaptation of the wound margins. Recognition and immediate management of lacrimal, canthal, and levator injuries and occult orbital foreign bodies may prevent late complications that are more difficult to repair.", "contents": "Treatment of acute eyelid trauma to prevent late complications. A checklist for preventing late complications of acute eyelid trauma should include recognizing injury to the globe, preventing infection, and achieving proper coaptation of the wound margins. Recognition and immediate management of lacrimal, canthal, and levator injuries and occult orbital foreign bodies may prevent late complications that are more difficult to repair.", "PMID": 960378}
{"id": "pubmed23n0032_3915", "title": "A profile of patients with intractable blepharospasm.", "content": "Of those patients seeking surgical correction for intractable blepharopasm, the spasm was more commonly bilateral than unilateral by a ratio of about 7:1 and the bilateral form was more common in women than men by a ratio of about 3:1. The unilateral disease had an equal sex incidence. The disease seemed to be the same in men and women. The median age of onset was 59 years old, and the median interval between onset of symptoms and surgery was three years. Most of these patients had seen many physicians, and all prior medical and surgical treatments had been unsuccessful.", "contents": "A profile of patients with intractable blepharospasm. Of those patients seeking surgical correction for intractable blepharopasm, the spasm was more commonly bilateral than unilateral by a ratio of about 7:1 and the bilateral form was more common in women than men by a ratio of about 3:1. The unilateral disease had an equal sex incidence. The disease seemed to be the same in men and women. The median age of onset was 59 years old, and the median interval between onset of symptoms and surgery was three years. Most of these patients had seen many physicians, and all prior medical and surgical treatments had been unsuccessful.", "PMID": 960381}
{"id": "pubmed23n0032_3916", "title": "Glaucoma in the beagle.", "content": "Glaucoma in the beagle is an inherited disease that is exhibited clinically in dogs of 6 to 18 months of age. The genealogy, clinical signs, gonioscopy, tonometry, tonography, water provocative response, and scanning electron microscopy of the iridocorneal angle of the glaucomatous beagle are summarized.", "contents": "Glaucoma in the beagle. Glaucoma in the beagle is an inherited disease that is exhibited clinically in dogs of 6 to 18 months of age. The genealogy, clinical signs, gonioscopy, tonometry, tonography, water provocative response, and scanning electron microscopy of the iridocorneal angle of the glaucomatous beagle are summarized.", "PMID": 960386}
{"id": "pubmed23n0032_3917", "title": "Keratoconjunctivitis sicca.", "content": "KCS in man is often associated with a systemic disease whose pathogenesis is still obscure. A spontaneous animal model resembling Sj\u00f6gren's syndrome has been developed in the NZB and NZB-NZW mouse. Other spontaneous animal models analagous to lupus erythematosis and polyarteritis have been found in dogs and Aleutian mink, respectively. Studies of the eyes and lacrimal glands in such animals, and in dogs with distemper, should be pursued. A viral etiology is implicated in each of the models. The roles of both the main and accessory lacrimal glands in normal and KCS eyes have been discussed, as well as drug-induced KCS and opportunities for its further investigation and treatment.", "contents": "Keratoconjunctivitis sicca. KCS in man is often associated with a systemic disease whose pathogenesis is still obscure. A spontaneous animal model resembling Sj\u00f6gren's syndrome has been developed in the NZB and NZB-NZW mouse. Other spontaneous animal models analagous to lupus erythematosis and polyarteritis have been found in dogs and Aleutian mink, respectively. Studies of the eyes and lacrimal glands in such animals, and in dogs with distemper, should be pursued. A viral etiology is implicated in each of the models. The roles of both the main and accessory lacrimal glands in normal and KCS eyes have been discussed, as well as drug-induced KCS and opportunities for its further investigation and treatment.", "PMID": 960384}
{"id": "pubmed23n0032_3918", "title": "Retinitis pigmentosa and allied retinal diseases: electrophysiologic findings.", "content": "The subnormal ERG can be used in young patients to establish the diagnosis of hereditary progressive forms of retinitis pigmentosa and to determine visual prognosis even when changes visible with the ophthalmoscope are minimal or absent. Different waveforms have been demonstrated in patients of comparable age with different genetic types. Defects must exist in the photoreceptors or pigmented epithelium or both to explain the reduced ERG a-wave amplitudes and reduced ERT amplitudes. Marked delays in cone or rod ERG b-wave implicit times or both reflect the wide-spread involvement of cone or rod photoreceptors or both, even in the early stages. These delays also have been recorded from the taurine-deficient cat with nutritionally induced retinal degeneration and from dogs with hereditary retinal degenerations. Attention should be given to those animals with retinal disease that have electrophysiologic abnormalities similar to those seen in patients with retinitis pigmentosa and allied degenerations.", "contents": "Retinitis pigmentosa and allied retinal diseases: electrophysiologic findings. The subnormal ERG can be used in young patients to establish the diagnosis of hereditary progressive forms of retinitis pigmentosa and to determine visual prognosis even when changes visible with the ophthalmoscope are minimal or absent. Different waveforms have been demonstrated in patients of comparable age with different genetic types. Defects must exist in the photoreceptors or pigmented epithelium or both to explain the reduced ERG a-wave amplitudes and reduced ERT amplitudes. Marked delays in cone or rod ERG b-wave implicit times or both reflect the wide-spread involvement of cone or rod photoreceptors or both, even in the early stages. These delays also have been recorded from the taurine-deficient cat with nutritionally induced retinal degeneration and from dogs with hereditary retinal degenerations. Attention should be given to those animals with retinal disease that have electrophysiologic abnormalities similar to those seen in patients with retinitis pigmentosa and allied degenerations.", "PMID": 960388}
{"id": "pubmed23n0032_3919", "title": "An examination of an electrochemical mechanism for noise-induced hair cell loss: noise with amino-oxyacetic acid (AOAA).", "content": "Pretreatment of guinea pigs with AOAA was found to reduce the incidence of hair cell loss due to noise exposure. Since AOAA reduces the endocochlear potential, we propose an electrochemical model as an extension of the Davis model to explain the protective effect of AOAA.", "contents": "An examination of an electrochemical mechanism for noise-induced hair cell loss: noise with amino-oxyacetic acid (AOAA). Pretreatment of guinea pigs with AOAA was found to reduce the incidence of hair cell loss due to noise exposure. Since AOAA reduces the endocochlear potential, we propose an electrochemical model as an extension of the Davis model to explain the protective effect of AOAA.", "PMID": 960397}
{"id": "pubmed23n0032_3920", "title": "Tone burst electrocochleography in humans.", "content": "Using tone burst stimuli with fixed parameters of rise time and duration, a functional relationship has been demonstrated between frequency and intensity of the stimulus and peak latency of electrocochleographic recordings. The feasibility of a \"map\" of the human cochlear partition has been shown for frequencies above 2 kHz.", "contents": "Tone burst electrocochleography in humans. Using tone burst stimuli with fixed parameters of rise time and duration, a functional relationship has been demonstrated between frequency and intensity of the stimulus and peak latency of electrocochleographic recordings. The feasibility of a \"map\" of the human cochlear partition has been shown for frequencies above 2 kHz.", "PMID": 960407}
{"id": "pubmed23n0032_3921", "title": "Masquerades of myocardial infarction.", "content": "I summarize these observations in Figure 1. It represents every person in a hypothetical population who has myocardial infarction. A large but unknown number, some believe almost half, never get help. Mobile coronary care units are reducing this group, but so far only a little. When the diagnosis is not understood the disease is not recognized. Then come discovery and popularization. Hereafter masquerades hide some cases and the diagnosis is missed. Somewhere fairly early the diagnostic fad leads to false positive diagnosis. As new techniques are discovered, perfected and mastered, false positive errors and masquerades leading to oversights diminish but still exist. All the skill and technical virtuosity in the world will not be applied if we do not think of the disease. When we think of it, even obscure cases may be resolved easily.", "contents": "Masquerades of myocardial infarction. I summarize these observations in Figure 1. It represents every person in a hypothetical population who has myocardial infarction. A large but unknown number, some believe almost half, never get help. Mobile coronary care units are reducing this group, but so far only a little. When the diagnosis is not understood the disease is not recognized. Then come discovery and popularization. Hereafter masquerades hide some cases and the diagnosis is missed. Somewhere fairly early the diagnostic fad leads to false positive diagnosis. As new techniques are discovered, perfected and mastered, false positive errors and masquerades leading to oversights diminish but still exist. All the skill and technical virtuosity in the world will not be applied if we do not think of the disease. When we think of it, even obscure cases may be resolved easily.", "PMID": 960416}
{"id": "pubmed23n0032_3922", "title": "Self-administered cardiopulmonary resuscitation by cough-induced cardiac compression.", "content": "Repeated rhythmic (every 1-3 sec.) coughs were documented to maintain consciousness up to 39 seconds in 3 patients developing ventricular fibrillation during coronary arteriography. The arterial pressure wave resulting from a cough exceeded that induced by external chest compression in 2 individuals in whom both techniques were employed and in 5 others treated by external compression alone. Cough-induced cardiac compression is self-performed, and compared to external chest compression is less likely to traumatize the chest wall or heart and can be performed in any position on any surface. It is recommended that patients undergoing coronary arteriography be previously trained to cough abruptly and repeatedly every 1-3 seconds.  The potential for utilizing this technique in other areas (i.e., CCU, home) is less favorable than in catheterization-induced ventricular fibrillation, but it might be employed successfully in patients with premonitory symptoms of ventricular arrhythmias or Stokes-Adams seizures. The prior training of high risk individuals (and their spouses) to induce effective coughing in the victim might be lifesaving.", "contents": "Self-administered cardiopulmonary resuscitation by cough-induced cardiac compression. Repeated rhythmic (every 1-3 sec.) coughs were documented to maintain consciousness up to 39 seconds in 3 patients developing ventricular fibrillation during coronary arteriography. The arterial pressure wave resulting from a cough exceeded that induced by external chest compression in 2 individuals in whom both techniques were employed and in 5 others treated by external compression alone. Cough-induced cardiac compression is self-performed, and compared to external chest compression is less likely to traumatize the chest wall or heart and can be performed in any position on any surface. It is recommended that patients undergoing coronary arteriography be previously trained to cough abruptly and repeatedly every 1-3 seconds.  The potential for utilizing this technique in other areas (i.e., CCU, home) is less favorable than in catheterization-induced ventricular fibrillation, but it might be employed successfully in patients with premonitory symptoms of ventricular arrhythmias or Stokes-Adams seizures. The prior training of high risk individuals (and their spouses) to induce effective coughing in the victim might be lifesaving.", "PMID": 960417}
{"id": "pubmed23n0032_3923", "title": "The Jeremiah Metzger lecture of The American Clinical and Climatological Association 1975. New genetic insight into old diseases.", "content": "With these three examples, examined in some detail, I have attempted to indicate new directions in medical genetics. Some of the recognizable generalities are the following: 1. With the application of cell culture techniques in the area called human somatic cell genetics, human genetics has become essentially an experimental science. Somaticcell studies have provided insight into genetic disorders that would not have been possible from studies in the whole organism. Even therapeutic possibilities can be explored. Somatic cell hyubridization has substituted for controlled matings in permitting linkage studies for mapping of the human chromosomes...", "contents": "The Jeremiah Metzger lecture of The American Clinical and Climatological Association 1975. New genetic insight into old diseases. With these three examples, examined in some detail, I have attempted to indicate new directions in medical genetics. Some of the recognizable generalities are the following: 1. With the application of cell culture techniques in the area called human somatic cell genetics, human genetics has become essentially an experimental science. Somaticcell studies have provided insight into genetic disorders that would not have been possible from studies in the whole organism. Even therapeutic possibilities can be explored. Somatic cell hyubridization has substituted for controlled matings in permitting linkage studies for mapping of the human chromosomes...", "PMID": 960419}
{"id": "pubmed23n0032_3924", "title": "Tracheostomy in the first year of life.", "content": "Three series totaling 301 infants under 1 year of age who had tracheotomies during three successive decades are reviewed. The first series (62 infants) was seen prior to 1953, the second (86 infants) was seen between 1954 and 1963, while the latest series (153 infants) was seen between 1964 and 1973. A comparison of the three series with reference to etiology, incidence, indications, management, and mortality leads to significant conclusions. (1) The incidence of tracheotomy is increasing. More aggressive approach to many problems, previously considered inoperable, resulted in an increased number of infants undergoing tracheotomy for postintubation problems and ventilatory support. (2) The incidence of congenital malformations of the airway necessitating tracheostomy has remained constant, while the downward trend in the incidence of inflammatory conditions was reversed. (3) Infants in the latest series underwent tracheotomy, on the average, at a later age than the 1954 to 1963 series, largely as a result of the increased number of patients with cardiac anomalies undergoing corrective cardiovascular surgery and subsequent tracheotomy at a later age. (4) A review of the 77 deaths in the latest series again shows the death rate to be dependent on the underlying systemic condition which necessitated tracheotomy, rather than the tracheostomy itself. (5) The importance of temporary intubation to relieve respiratory obstruction in infants is stressed, while the significant laryngeal damage following prolonged intubation and delayed tracheotomy is apparent.", "contents": "Tracheostomy in the first year of life. Three series totaling 301 infants under 1 year of age who had tracheotomies during three successive decades are reviewed. The first series (62 infants) was seen prior to 1953, the second (86 infants) was seen between 1954 and 1963, while the latest series (153 infants) was seen between 1964 and 1973. A comparison of the three series with reference to etiology, incidence, indications, management, and mortality leads to significant conclusions. (1) The incidence of tracheotomy is increasing. More aggressive approach to many problems, previously considered inoperable, resulted in an increased number of infants undergoing tracheotomy for postintubation problems and ventilatory support. (2) The incidence of congenital malformations of the airway necessitating tracheostomy has remained constant, while the downward trend in the incidence of inflammatory conditions was reversed. (3) Infants in the latest series underwent tracheotomy, on the average, at a later age than the 1954 to 1963 series, largely as a result of the increased number of patients with cardiac anomalies undergoing corrective cardiovascular surgery and subsequent tracheotomy at a later age. (4) A review of the 77 deaths in the latest series again shows the death rate to be dependent on the underlying systemic condition which necessitated tracheotomy, rather than the tracheostomy itself. (5) The importance of temporary intubation to relieve respiratory obstruction in infants is stressed, while the significant laryngeal damage following prolonged intubation and delayed tracheotomy is apparent.", "PMID": 960414}
{"id": "pubmed23n0032_3925", "title": "Histoplasmosis: twenty years experience in a general hospital.", "content": "This report summarizes the clinical and laboratory data on 57 patients with histoplasmosis, including 13 with progressive disseminated disease. The experience in general is supportive of that of others in that although infection with Histoplasma capsulatum is prevalent, clinical disease is uncommon and disseminated disease actually rare. The data again emphasize the limitations of diagnostic methods, and in particular point up the need to obtain cultures of biopsy material from mucocutaneous lesions.", "contents": "Histoplasmosis: twenty years experience in a general hospital. This report summarizes the clinical and laboratory data on 57 patients with histoplasmosis, including 13 with progressive disseminated disease. The experience in general is supportive of that of others in that although infection with Histoplasma capsulatum is prevalent, clinical disease is uncommon and disseminated disease actually rare. The data again emphasize the limitations of diagnostic methods, and in particular point up the need to obtain cultures of biopsy material from mucocutaneous lesions.", "PMID": 960420}
{"id": "pubmed23n0032_3926", "title": "Left ventricular performance in coronary artery disease by systolic time intervals and echocardiography.", "content": "Determinations of systolic time intervals and echocardiographic measures of left ventricular performance were made in twenty-five normal subjects and 37 patients who had convalesced from a previous transmural myocardial infarction. On group analysis the patients with previous myocardial infarction demonstrated significant differences from the normals in each of the noninvasive measures. Among the noninvasive measures the PEP/LVET and deltaD% proved to be the most sensitive indicators of left ventricular dysfunction. Deviations from the normal range in PEP/LVET and deltaD% occurred in 65% to 70% of patients asymptomatic for dyspnea and fatigability (20 patients) and in 85% of patients asymptomatic for angina pectoris (13 patients). Neither the presence of phonocardiographically documented S3 or S4 or the finding of an abnormal C/T ratio on standard chest x-ray reliably detected patients with abnormal left ventricular performance. Among 26 patients studied by coronary arteriography, abnormal left ventricular performance by PEP/LVET and deltaD% occurred in less than 30% of those with obstruction (70% or greater) of one coronary artery and in over 80% of those with obstruction of two or three major arteries. A close correlation existed between the level of left ventricular performance measured by the PEP/LVET and the deltaD% (r = 0.93). These studies document the high degree of sensitivity of the noninvasive measures and demonstrate their superiority over clinical methods for detecting abnormal left ventricular performance in patients with previous myocardial infarction.", "contents": "Left ventricular performance in coronary artery disease by systolic time intervals and echocardiography. Determinations of systolic time intervals and echocardiographic measures of left ventricular performance were made in twenty-five normal subjects and 37 patients who had convalesced from a previous transmural myocardial infarction. On group analysis the patients with previous myocardial infarction demonstrated significant differences from the normals in each of the noninvasive measures. Among the noninvasive measures the PEP/LVET and deltaD% proved to be the most sensitive indicators of left ventricular dysfunction. Deviations from the normal range in PEP/LVET and deltaD% occurred in 65% to 70% of patients asymptomatic for dyspnea and fatigability (20 patients) and in 85% of patients asymptomatic for angina pectoris (13 patients). Neither the presence of phonocardiographically documented S3 or S4 or the finding of an abnormal C/T ratio on standard chest x-ray reliably detected patients with abnormal left ventricular performance. Among 26 patients studied by coronary arteriography, abnormal left ventricular performance by PEP/LVET and deltaD% occurred in less than 30% of those with obstruction (70% or greater) of one coronary artery and in over 80% of those with obstruction of two or three major arteries. A close correlation existed between the level of left ventricular performance measured by the PEP/LVET and the deltaD% (r = 0.93). These studies document the high degree of sensitivity of the noninvasive measures and demonstrate their superiority over clinical methods for detecting abnormal left ventricular performance in patients with previous myocardial infarction.", "PMID": 960423}
{"id": "pubmed23n0032_3927", "title": "[Resection and plastic surgery of the arteries in malignant tumors of soft tissues of the lower limbs].", "content": "An experience of the vascular surgery department with reconstructive interventions on large arteries of patients with malignant tumors of the lower extremities soft tissues, located in the zone of large vessels of the inguinal region, femur and popliteal fossa, is reported. According to the author's opinion, it is necessary to take into account the fact that in reconstructive procedures on large arteries in patients, subjected to massive repeated radiotherapy, there is an increased danger of occurrence of arrosion hemorrhage, extensive thrombosis of vascular grafts and arteries changed under the effect of ionizing radiation.", "contents": "[Resection and plastic surgery of the arteries in malignant tumors of soft tissues of the lower limbs]. An experience of the vascular surgery department with reconstructive interventions on large arteries of patients with malignant tumors of the lower extremities soft tissues, located in the zone of large vessels of the inguinal region, femur and popliteal fossa, is reported. According to the author's opinion, it is necessary to take into account the fact that in reconstructive procedures on large arteries in patients, subjected to massive repeated radiotherapy, there is an increased danger of occurrence of arrosion hemorrhage, extensive thrombosis of vascular grafts and arteries changed under the effect of ionizing radiation.", "PMID": 960447}
{"id": "pubmed23n0032_3928", "title": "[Clinical and experimental grounds for the method of spontaneous respiration with difficult exhalation (lung inflation) in children].", "content": "Three methods of inducing difficulty in breathing out in spontaneous ventilation are described: by means of a valve attached to an intubation tube (I), with a polyethylene sac secured hermetically on patient's head and connected to a source of oxygen (2), and also with the help of a low-pressure chamber for patient's body (3). Experiments and clinical observations (40 therapeutic seances in 23 children aged from 1 day to 7 years) have evidenced that this method is mostly more advantageous than artificial ventilation, it is highly efficient in treatment of aspiration pneumonia and pulmonary edema, also it may be used with prophylactic purposes following aspiration, artificial ventilation, in the immediate postoperative period, and after hyperbaric oxygenation.", "contents": "[Clinical and experimental grounds for the method of spontaneous respiration with difficult exhalation (lung inflation) in children]. Three methods of inducing difficulty in breathing out in spontaneous ventilation are described: by means of a valve attached to an intubation tube (I), with a polyethylene sac secured hermetically on patient's head and connected to a source of oxygen (2), and also with the help of a low-pressure chamber for patient's body (3). Experiments and clinical observations (40 therapeutic seances in 23 children aged from 1 day to 7 years) have evidenced that this method is mostly more advantageous than artificial ventilation, it is highly efficient in treatment of aspiration pneumonia and pulmonary edema, also it may be used with prophylactic purposes following aspiration, artificial ventilation, in the immediate postoperative period, and after hyperbaric oxygenation.", "PMID": 960448}
{"id": "pubmed23n0032_3929", "title": "[Obstruction of the vesico-urethral segment in children].", "content": "Within a 5-year period (1970-1974) in the Child Surgery Clinic of Leningrad Pediatric Medical Institute 63 children, aged from 2 months to 14 years (55 boys and 8 girls), were treated for the obstruction of the vesico-urethral segment. It is believed that timely performed surgical removal of the obstruction with simultaneous resection if a non-functioning kidney with the phenomena of vesico-urethral reflux allows an effective treatment of secondary pyelonephritis.", "contents": "[Obstruction of the vesico-urethral segment in children]. Within a 5-year period (1970-1974) in the Child Surgery Clinic of Leningrad Pediatric Medical Institute 63 children, aged from 2 months to 14 years (55 boys and 8 girls), were treated for the obstruction of the vesico-urethral segment. It is believed that timely performed surgical removal of the obstruction with simultaneous resection if a non-functioning kidney with the phenomena of vesico-urethral reflux allows an effective treatment of secondary pyelonephritis.", "PMID": 960449}
{"id": "pubmed23n0032_3930", "title": "[Importance of structural characteristics of the esophagus and tracheo-esophageal fistula in surgical treatment of esophageal atresia in newborn infants].", "content": "The anatomical structure of the upper and lower segments of the esophagus and tracheoesophageal fistula in esophageal atresia in newborn in newborn infants conditions the clinical picture, character of surgical intervention and postoperative course. The gravity of pneumonic changes in the lung is dependent not only on terms of child's admission, but also on the width of esophagotracheal fistula, while bifurcation fistulas, sometimes, cause diagnostic erros.", "contents": "[Importance of structural characteristics of the esophagus and tracheo-esophageal fistula in surgical treatment of esophageal atresia in newborn infants]. The anatomical structure of the upper and lower segments of the esophagus and tracheoesophageal fistula in esophageal atresia in newborn in newborn infants conditions the clinical picture, character of surgical intervention and postoperative course. The gravity of pneumonic changes in the lung is dependent not only on terms of child's admission, but also on the width of esophagotracheal fistula, while bifurcation fistulas, sometimes, cause diagnostic erros.", "PMID": 960450}
{"id": "pubmed23n0032_3931", "title": "[Pellegrini-Stieda disease (shadow)].", "content": "The Pellegrini-Stied disease (shadow) (calcification periarthritis of the knee) is characterized by the presence of ossification of the medial condyl of the femur. The author's observations of 51 patients (sportsmen) suffering this lesion are described. The methods of conservative and operative treatment and the results obtained are reported.", "contents": "[Pellegrini-Stieda disease (shadow)]. The Pellegrini-Stied disease (shadow) (calcification periarthritis of the knee) is characterized by the presence of ossification of the medial condyl of the femur. The author's observations of 51 patients (sportsmen) suffering this lesion are described. The methods of conservative and operative treatment and the results obtained are reported.", "PMID": 960451}
{"id": "pubmed23n0032_3932", "title": "[Remote results of treatment of injuries of large arteries of the limbs].", "content": "The results of treatment of 115 patients with vascular injuries were studied within the terms from 3 to 20 years after the operation. In 7 patients the vessels were ligated, in the remainder--repaired. The functional state of the extremity and blood vessels was studied using oscillo- and rheography, angiography, ergometry and other methods. In 81 patients (70.5%) the extremity function was normal, in 24 (20.8%)--moderately restricted, and in 10 (8.7%)--poor.", "contents": "[Remote results of treatment of injuries of large arteries of the limbs]. The results of treatment of 115 patients with vascular injuries were studied within the terms from 3 to 20 years after the operation. In 7 patients the vessels were ligated, in the remainder--repaired. The functional state of the extremity and blood vessels was studied using oscillo- and rheography, angiography, ergometry and other methods. In 81 patients (70.5%) the extremity function was normal, in 24 (20.8%)--moderately restricted, and in 10 (8.7%)--poor.", "PMID": 960452}
{"id": "pubmed23n0032_3933", "title": "[Microclots of perfusion mixtures during open heart surgery with artificial circulation].", "content": "During 9 experimental and 93 clinical operations on the open heart under circulatory bypass the authors studied microclots of multicomponent perfusion mixtures. They observed an increased number of microclots after infusion of high-molecular plasma-substituting solutions and long-term intraapparatus recirculation. During the bypass there was an increased number of microclots in the \"machine-patient\" system. The complex of measures suggested by the authors contributes to a delay in additional formation of microclots during the perfusion and also reduces the number of posttransfusion complications.", "contents": "[Microclots of perfusion mixtures during open heart surgery with artificial circulation]. During 9 experimental and 93 clinical operations on the open heart under circulatory bypass the authors studied microclots of multicomponent perfusion mixtures. They observed an increased number of microclots after infusion of high-molecular plasma-substituting solutions and long-term intraapparatus recirculation. During the bypass there was an increased number of microclots in the \"machine-patient\" system. The complex of measures suggested by the authors contributes to a delay in additional formation of microclots during the perfusion and also reduces the number of posttransfusion complications.", "PMID": 960460}
{"id": "pubmed23n0032_3934", "title": "[Prevention and treatment of postoperative broncho-pulmonary complications with ultrasonic aerosols].", "content": "The method and results of applying ultrasonic aerosols in 485 patients, operated upon on abdominal organs, are presented. Ultrasonic aerosols were included in the complex of prophylactic and therapeutic measures in the postoperative period. Prophylactic measures were started immediately after patient's being returned from the operating room. Special attention was given to patients in whom ventilatory disturbances developed during anesthesia, to patients with obesity, chronic bronchitis, emphysema, pneumosclerosis and also those, who negated the rationality of prophylactic measures. The use of finely dispersed aerosols after the method suggested by the authors allowed a 6 times reduction in the incidence of pulmonary complications and more than twice shortening of the postoperative pneumonia course.", "contents": "[Prevention and treatment of postoperative broncho-pulmonary complications with ultrasonic aerosols]. The method and results of applying ultrasonic aerosols in 485 patients, operated upon on abdominal organs, are presented. Ultrasonic aerosols were included in the complex of prophylactic and therapeutic measures in the postoperative period. Prophylactic measures were started immediately after patient's being returned from the operating room. Special attention was given to patients in whom ventilatory disturbances developed during anesthesia, to patients with obesity, chronic bronchitis, emphysema, pneumosclerosis and also those, who negated the rationality of prophylactic measures. The use of finely dispersed aerosols after the method suggested by the authors allowed a 6 times reduction in the incidence of pulmonary complications and more than twice shortening of the postoperative pneumonia course.", "PMID": 960462}
{"id": "pubmed23n0032_3935", "title": "[Function of the lungs after tracheo-bronchial resections].", "content": "Experimentally on 20 dogs, using the method of xenon-133 radiography, angiopneumo- and bronchography the lung function was studied after tracheobronchial resections, performed in young ages. The pulmonary function was not found to be appreciably changed, the observed alterations are of a compensatory character.", "contents": "[Function of the lungs after tracheo-bronchial resections]. Experimentally on 20 dogs, using the method of xenon-133 radiography, angiopneumo- and bronchography the lung function was studied after tracheobronchial resections, performed in young ages. The pulmonary function was not found to be appreciably changed, the observed alterations are of a compensatory character.", "PMID": 960463}
{"id": "pubmed23n0032_3936", "title": "[Age aspects of remote outcome of acute lung suppuration].", "content": "Among 375 patients (adults and children) having acute suppurative processes in the lung, treated conservatively, 165 were examined in later terms up to 15 years, using modern bronchological methods, considerable changes being revealed in 66.2%: residual cavities, chronic abscesses, bronchitis deformans and bronchiectases. In children suppuration in the lung showed a more grave course, but reparative processes were much better pronounced. The studies conducted allowed a conclusion on the rationality of widening indications to radical surgical treatment in adult patients and the necessity of a dispensary control over children and adult patients, including those discharged with a recovery.", "contents": "[Age aspects of remote outcome of acute lung suppuration]. Among 375 patients (adults and children) having acute suppurative processes in the lung, treated conservatively, 165 were examined in later terms up to 15 years, using modern bronchological methods, considerable changes being revealed in 66.2%: residual cavities, chronic abscesses, bronchitis deformans and bronchiectases. In children suppuration in the lung showed a more grave course, but reparative processes were much better pronounced. The studies conducted allowed a conclusion on the rationality of widening indications to radical surgical treatment in adult patients and the necessity of a dispensary control over children and adult patients, including those discharged with a recovery.", "PMID": 960464}
{"id": "pubmed23n0032_3937", "title": "[Surgical treatment of aortic coarctation in adults].", "content": "During a 18-year period the authors operated 305 patients with aortic coarctation, the mortality being 2.2%. Sixty three patients (20.6%) aged from 20 to 40 years were operated upon with the mortality in 7.9%. A direct anastomosis of the aorta was constructed in 31 patients, alloprosthesis of the aorta was employed in 23 cases, indirect isthmoplasty--in 8 cases, in one case an aortic prosthesis was replaced. The most frequent intra- and postoperative complication was intrapleural hemorrhage. In estimating the efficacy of surgery within the terms up to 14 years the best results were obtained after an end-to-end anastomosis.", "contents": "[Surgical treatment of aortic coarctation in adults]. During a 18-year period the authors operated 305 patients with aortic coarctation, the mortality being 2.2%. Sixty three patients (20.6%) aged from 20 to 40 years were operated upon with the mortality in 7.9%. A direct anastomosis of the aorta was constructed in 31 patients, alloprosthesis of the aorta was employed in 23 cases, indirect isthmoplasty--in 8 cases, in one case an aortic prosthesis was replaced. The most frequent intra- and postoperative complication was intrapleural hemorrhage. In estimating the efficacy of surgery within the terms up to 14 years the best results were obtained after an end-to-end anastomosis.", "PMID": 960465}
{"id": "pubmed23n0032_3938", "title": "[Measurement of thoracic complex electrical resistance (impedance)].", "content": "Experimental grounds for using clinically the chest impedence recording are presented. The method allows recording of ventilatory and hemodynamic parameters mainly in the minor circulatory circuit under conditions of surgery on thoracic organs and postoperatively, as well.", "contents": "[Measurement of thoracic complex electrical resistance (impedance)]. Experimental grounds for using clinically the chest impedence recording are presented. The method allows recording of ventilatory and hemodynamic parameters mainly in the minor circulatory circuit under conditions of surgery on thoracic organs and postoperatively, as well.", "PMID": 960466}
{"id": "pubmed23n0032_3939", "title": "[Functional state and structure of gastric mucosa in patients with ulcer disease after vagotomy and pyloroplasty].", "content": "107 patients were examined in different terms after vagotomy (up to 6 years) performed for ulcerous disease of the duodenum, 43 of them--prior to and after the operation. It is found that the suppression of the acid- and enzyme secretory function of the stomach is not related to the development of the mucosa atrophy, but it is conditioned by eliminating the resolving role of vagus nerves and by a reduced sensitivity of fundic glands to humoral stimuli.", "contents": "[Functional state and structure of gastric mucosa in patients with ulcer disease after vagotomy and pyloroplasty]. 107 patients were examined in different terms after vagotomy (up to 6 years) performed for ulcerous disease of the duodenum, 43 of them--prior to and after the operation. It is found that the suppression of the acid- and enzyme secretory function of the stomach is not related to the development of the mucosa atrophy, but it is conditioned by eliminating the resolving role of vagus nerves and by a reduced sensitivity of fundic glands to humoral stimuli.", "PMID": 960467}
{"id": "pubmed23n0032_3940", "title": "[Large stump of the cystic duct].", "content": "The authors concluded that a large stump of the gall-bladder duct is of no independent importance as a cause of the affection. However, concretions may be formed in the stump, that was evident in three cases, therefore, the gall duct stump should be no more than 0.5 cm.", "contents": "[Large stump of the cystic duct]. The authors concluded that a large stump of the gall-bladder duct is of no independent importance as a cause of the affection. However, concretions may be formed in the stump, that was evident in three cases, therefore, the gall duct stump should be no more than 0.5 cm.", "PMID": 960468}
{"id": "pubmed23n0032_3941", "title": "[Benzoylarginine amidase activity of blood serum and plasma in acute pancreatitis].", "content": "In 40 patients with acute pancreatitis, examined 3-2 and 18-24 hours following the onset of the disease, benzoylarginine-amidase activity in blood serum and plasma was determined, as well as the activity of lipase and amylase in blood serum. A correlative dependence was found between the activity of amylase and lipase in blood serum, the activity of amylase in blood serum and trypsin-like activity in blood plasma of patients investigated in different time.", "contents": "[Benzoylarginine amidase activity of blood serum and plasma in acute pancreatitis]. In 40 patients with acute pancreatitis, examined 3-2 and 18-24 hours following the onset of the disease, benzoylarginine-amidase activity in blood serum and plasma was determined, as well as the activity of lipase and amylase in blood serum. A correlative dependence was found between the activity of amylase and lipase in blood serum, the activity of amylase in blood serum and trypsin-like activity in blood plasma of patients investigated in different time.", "PMID": 960469}
{"id": "pubmed23n0032_3942", "title": "[Perforative peritonitis as a complication of abdominal tuberculosis].", "content": "In the paper, data on 502 patients with abdominal tuberculosis are reported, including 21--with perforative peritonitis due to the perforation of tuberculous intestinal ulcers. In its manifestations perforative peritonitis may show a latent or \"an acute abdomen\" course. Among 12 operated patients there were 5 lethal issues. Such high postoperative mortality is accounted for late terms of establishing the correct diagnosis and admission of patients in a poor and hopeless condition.", "contents": "[Perforative peritonitis as a complication of abdominal tuberculosis]. In the paper, data on 502 patients with abdominal tuberculosis are reported, including 21--with perforative peritonitis due to the perforation of tuberculous intestinal ulcers. In its manifestations perforative peritonitis may show a latent or \"an acute abdomen\" course. Among 12 operated patients there were 5 lethal issues. Such high postoperative mortality is accounted for late terms of establishing the correct diagnosis and admission of patients in a poor and hopeless condition.", "PMID": 960470}
{"id": "pubmed23n0032_3943", "title": "[Evaluation of the changes of natural resistance in patients with diffuse peritonitis].", "content": "A suppression of humoral and cell mechanisms of natural nonspecific resistance was found in patients with diffuse peritonitis. A considerable degranulation of basophilic leucocytes was indicated by the direct Shelley reaction, that evidences alterations in the functional state of these cells. Postoperatively, there is a break in restoration of the activity of properdine and its cofactors, and a considerable suppression of the phagocytic capacity of microphages is noted, that leads inevitably to a reduction of immune reactivity.", "contents": "[Evaluation of the changes of natural resistance in patients with diffuse peritonitis]. A suppression of humoral and cell mechanisms of natural nonspecific resistance was found in patients with diffuse peritonitis. A considerable degranulation of basophilic leucocytes was indicated by the direct Shelley reaction, that evidences alterations in the functional state of these cells. Postoperatively, there is a break in restoration of the activity of properdine and its cofactors, and a considerable suppression of the phagocytic capacity of microphages is noted, that leads inevitably to a reduction of immune reactivity.", "PMID": 960471}
{"id": "pubmed23n0032_3944", "title": "[Extraorganic forms of abdominal localization of echinococcosis].", "content": "The author has analysed 66 pateints (5.4% of all cases of the disease) with extraorganic abdominal echinococcosis. The forms of retroperitoneal (lumbar, pelvic, mesenteric and omental) and intraperitoneal echinococcosis (primary cystic and adhesive-disseminated) are differentiated. The problems of diagnosis, the differential diagnosis and selection of an appropriate therapy are discussed. The mortality in multiple and associated affections was within the range of 10 per cent.", "contents": "[Extraorganic forms of abdominal localization of echinococcosis]. The author has analysed 66 pateints (5.4% of all cases of the disease) with extraorganic abdominal echinococcosis. The forms of retroperitoneal (lumbar, pelvic, mesenteric and omental) and intraperitoneal echinococcosis (primary cystic and adhesive-disseminated) are differentiated. The problems of diagnosis, the differential diagnosis and selection of an appropriate therapy are discussed. The mortality in multiple and associated affections was within the range of 10 per cent.", "PMID": 960472}
{"id": "pubmed23n0032_3945", "title": "[Disorders and correction of lymph outflow in chronic venous insufficiency of the lower limbs].", "content": "An analysis of 176 lymphograms, made in 102 patients with venous pathology of the lower extremities, is presented. In 14 of them varicosity in a decompensation stage, an uncomplicated form, in 32--varicosity in a decompensation stage, a complicated form, in 20--postthrombotic disease of an edematous and algesic form, in 36--an edematous-ulcerous form. The duration of the disease was from 3 to 25 years. It was found that the depth of changes in the lymphatic system was dependent on the rate of decompensation in the venous one.", "contents": "[Disorders and correction of lymph outflow in chronic venous insufficiency of the lower limbs]. An analysis of 176 lymphograms, made in 102 patients with venous pathology of the lower extremities, is presented. In 14 of them varicosity in a decompensation stage, an uncomplicated form, in 32--varicosity in a decompensation stage, a complicated form, in 20--postthrombotic disease of an edematous and algesic form, in 36--an edematous-ulcerous form. The duration of the disease was from 3 to 25 years. It was found that the depth of changes in the lymphatic system was dependent on the rate of decompensation in the venous one.", "PMID": 960473}
{"id": "pubmed23n0032_3946", "title": "[Reconstructive operations in post-thrombophlebitis syndrome of the pelvis and the lower limbs].", "content": "Based on the analysis of serial phlebograms (in 155 patients), kinematophlebograms (in 51 patients) and operative findings (in 42 patients), the authors differentiate 7 types of localization of venous occlusions of the lower extremities and pelvis in post-thrombophlebitic syndrome. The suggested classification is an aid in selecting the technic of surgical intervention. Further, the authors share their experience with reconstructive operations on the venous system in postthrombophlebitic syndrome of the lower extremities in 40 patients.", "contents": "[Reconstructive operations in post-thrombophlebitis syndrome of the pelvis and the lower limbs]. Based on the analysis of serial phlebograms (in 155 patients), kinematophlebograms (in 51 patients) and operative findings (in 42 patients), the authors differentiate 7 types of localization of venous occlusions of the lower extremities and pelvis in post-thrombophlebitic syndrome. The suggested classification is an aid in selecting the technic of surgical intervention. Further, the authors share their experience with reconstructive operations on the venous system in postthrombophlebitic syndrome of the lower extremities in 40 patients.", "PMID": 960474}
{"id": "pubmed23n0032_3947", "title": "[Characteristics of disorders of body functions in post-thrombophlebitic disease].", "content": "397 patients with chronic diseases of the extremities veins were examined, 256 of them suffered postthrombophlebitic disease. Along with the general clinical examination special investigations were conducted: ECG under orthostatic stress conditions, test tube and instrumental auto-coagulograms, thromboelastograms, biomicroscopy of the anterior ocular segment, studies of the intraocular pressure, ocular fundus, rheovasography, skin thermometry, oscillometry (with nitroglycerin stress), the extremity volume under orthostatic stress conditions, myography, non-contrast roentgenography of the lower extremities, and also the phenomenon of intradermal diffusion and pheblography. It was found that with the progress of postthrombophlebitis trophic disorders are observed in all tissues of the involved extremity. Postthrombophlebitic disease is associated not only with local involvement of vessels, but also with functional disturbances of other organs.", "contents": "[Characteristics of disorders of body functions in post-thrombophlebitic disease]. 397 patients with chronic diseases of the extremities veins were examined, 256 of them suffered postthrombophlebitic disease. Along with the general clinical examination special investigations were conducted: ECG under orthostatic stress conditions, test tube and instrumental auto-coagulograms, thromboelastograms, biomicroscopy of the anterior ocular segment, studies of the intraocular pressure, ocular fundus, rheovasography, skin thermometry, oscillometry (with nitroglycerin stress), the extremity volume under orthostatic stress conditions, myography, non-contrast roentgenography of the lower extremities, and also the phenomenon of intradermal diffusion and pheblography. It was found that with the progress of postthrombophlebitis trophic disorders are observed in all tissues of the involved extremity. Postthrombophlebitic disease is associated not only with local involvement of vessels, but also with functional disturbances of other organs.", "PMID": 960475}
{"id": "pubmed23n0032_3948", "title": "[Surgical treatment of congenital hemolytic anemia in children].", "content": "In the paper, an experience with surgical treatment of 67 children suffering inherited spherocytosis is summarized. In most patients the first signs of the disease appeared in early age. It is concluded that splenectomy is indicated in all children with inherited spherocytosis irrespective of the form of the disease at the age from 3 to 5 years. In a grave course and the appearance of signs of functional hepatic insufficiency the operation should be accomplished as earlier as posisble.", "contents": "[Surgical treatment of congenital hemolytic anemia in children]. In the paper, an experience with surgical treatment of 67 children suffering inherited spherocytosis is summarized. In most patients the first signs of the disease appeared in early age. It is concluded that splenectomy is indicated in all children with inherited spherocytosis irrespective of the form of the disease at the age from 3 to 5 years. In a grave course and the appearance of signs of functional hepatic insufficiency the operation should be accomplished as earlier as posisble.", "PMID": 960476}
{"id": "pubmed23n0032_3949", "title": "[Parellel blood circulation with oxygenation of blood in severe poisoning with carbon monoxide fumes].", "content": "The authors present an analysis of treatment in 141 patients with acute carboxyhemoglobinemia, in 110 of them severe intoxication was noted. All these patients were treated taking into account the degree of intoxication, the period of time elapsed since the moment of the intoxication till the onset of therapy, and the presence of biochemical shifts in blood. In 31 patients being in an extremely poor comatous state with considerable disorders in gas balance, persistant hemodynamic disturbances and manifest humoral shifts a parallel perfusion with extracorporeal oxygenation of blood was performed with medical drug therapy in the background. The immediate effect was found to be quite favourable, however, 19 patients died in different terms as a result of subsequent complications, most frequently due to pneumonia and meningoencephalitis.", "contents": "[Parellel blood circulation with oxygenation of blood in severe poisoning with carbon monoxide fumes]. The authors present an analysis of treatment in 141 patients with acute carboxyhemoglobinemia, in 110 of them severe intoxication was noted. All these patients were treated taking into account the degree of intoxication, the period of time elapsed since the moment of the intoxication till the onset of therapy, and the presence of biochemical shifts in blood. In 31 patients being in an extremely poor comatous state with considerable disorders in gas balance, persistant hemodynamic disturbances and manifest humoral shifts a parallel perfusion with extracorporeal oxygenation of blood was performed with medical drug therapy in the background. The immediate effect was found to be quite favourable, however, 19 patients died in different terms as a result of subsequent complications, most frequently due to pneumonia and meningoencephalitis.", "PMID": 960479}
{"id": "pubmed23n0032_3950", "title": "[Use of biological antiseptic tampon (BAT) for treatment of infected wounds and fistulas after abdominal surgery].", "content": "In the paper, based on the experience with the treatment of 42 patients having extensive purulent wounds and fistulas, the use of a biologial antiseptic tampon (BAT) for treatment of persistantly unhealing wounds and fistulas after various abdominal operations is discussed. The mechanism of BAT therapeutic effect, the method of its application and the results of treatment are described.", "contents": "[Use of biological antiseptic tampon (BAT) for treatment of infected wounds and fistulas after abdominal surgery]. In the paper, based on the experience with the treatment of 42 patients having extensive purulent wounds and fistulas, the use of a biologial antiseptic tampon (BAT) for treatment of persistantly unhealing wounds and fistulas after various abdominal operations is discussed. The mechanism of BAT therapeutic effect, the method of its application and the results of treatment are described.", "PMID": 960487}
{"id": "pubmed23n0032_3951", "title": "[Accessory artificial circulation in complex treatment of experimental acute toxic pulmonary edema].", "content": "In experiments on 50 dogs with toxic acute edema of the lung, induced with intravenous injection of 0.1% silver nitrate, the authors have studied the efficacy of accessory artificial circulation and \"conservative\" therapy. During the perfusion a discharge of the right portions, adequate extracorporeal gas metabolism, normalization of blood gas and acid-base balance were noted; an intensity of pulmonary edema is descreased. An intensive therapy for pulmonary edema was found to be more effective in association of \"conservative\" treatment with venoarterial perfusion and blood oxygenation.", "contents": "[Accessory artificial circulation in complex treatment of experimental acute toxic pulmonary edema]. In experiments on 50 dogs with toxic acute edema of the lung, induced with intravenous injection of 0.1% silver nitrate, the authors have studied the efficacy of accessory artificial circulation and \"conservative\" therapy. During the perfusion a discharge of the right portions, adequate extracorporeal gas metabolism, normalization of blood gas and acid-base balance were noted; an intensity of pulmonary edema is descreased. An intensive therapy for pulmonary edema was found to be more effective in association of \"conservative\" treatment with venoarterial perfusion and blood oxygenation.", "PMID": 960488}
{"id": "pubmed23n0032_3952", "title": "[Early adhesive intestinal obstruction].", "content": "In the paper, on the basis of 54 observations the incidence of early adhesive intestinal obstruction, its diagnosis and operative treatment are discussed. In case of jejunal overfilling a modified I. D. Zhitnjuk enterostomy is recommended, the lessens the danger of the operative wound contamination, accelerates an insertion of a drainage tube in the intestinal lumen and provides an opportunity to accomplish continuous suction both during the process of intestinal drainage and in the postoperative period.", "contents": "[Early adhesive intestinal obstruction]. In the paper, on the basis of 54 observations the incidence of early adhesive intestinal obstruction, its diagnosis and operative treatment are discussed. In case of jejunal overfilling a modified I. D. Zhitnjuk enterostomy is recommended, the lessens the danger of the operative wound contamination, accelerates an insertion of a drainage tube in the intestinal lumen and provides an opportunity to accomplish continuous suction both during the process of intestinal drainage and in the postoperative period.", "PMID": 960489}
{"id": "pubmed23n0032_3953", "title": "[Dialectics of the objective and subjective in the diagnosis of diseases].", "content": "The reader's attention is drawn to the fact that the dialectics of the objective and subjective in the cognition process is one of the most important and \"enternal\" philosophic problems. At each historical step of development of the science and public practice, in each sphere or cognition activity the former is of a definite specificity. It is the authors' opinion that the same is fully refered to such complicated field of cognition as the diagnosis of diseases.", "contents": "[Dialectics of the objective and subjective in the diagnosis of diseases]. The reader's attention is drawn to the fact that the dialectics of the objective and subjective in the cognition process is one of the most important and \"enternal\" philosophic problems. At each historical step of development of the science and public practice, in each sphere or cognition activity the former is of a definite specificity. It is the authors' opinion that the same is fully refered to such complicated field of cognition as the diagnosis of diseases.", "PMID": 960490}
{"id": "pubmed23n0032_3954", "title": "[Surgical importance of the topography of the vessels and bile ducts in the hepatic portal in case of their atypical branching and formation].", "content": "The portal triad was studied on 100 specimens of porta hepatis, using the methods of preparation, roentgenography and corrosion. An unusual origin of hepatic arteries was observed in 36%, atypical formation of bile ducts--in 33%, and a specific branching of the portal vein--in 15%. Different variants of vessels and bile ducts related with their specific development can be intercombined. Due to it, during surgical procedures in the region of porta hepatis it is necessary to bear in mind not only an atypical structure of each element of the Glisson system, but also the possibility of frequent combination of their different variants.", "contents": "[Surgical importance of the topography of the vessels and bile ducts in the hepatic portal in case of their atypical branching and formation]. The portal triad was studied on 100 specimens of porta hepatis, using the methods of preparation, roentgenography and corrosion. An unusual origin of hepatic arteries was observed in 36%, atypical formation of bile ducts--in 33%, and a specific branching of the portal vein--in 15%. Different variants of vessels and bile ducts related with their specific development can be intercombined. Due to it, during surgical procedures in the region of porta hepatis it is necessary to bear in mind not only an atypical structure of each element of the Glisson system, but also the possibility of frequent combination of their different variants.", "PMID": 960491}
{"id": "pubmed23n0032_3955", "title": "[Functional morphology of the liver in obstructive jaundice].", "content": "The conducted clinico-experimental study (an investigation of the functional and morphological state of the liver in impaired bile outflow) indicated different compensatory reactions. It is the authors' opinion that bile congestion in the liver is accompanied with proliferation of bile ducts; a damaging effect of bile components, in the first place bilious acids, is manifested in pericholangitis, periductal fibrosis and later in the development of biliary cirrhosis; alterations of the parenchyma occur secondarily also as a result of the toxic effect of bilous components and show a focal character.", "contents": "[Functional morphology of the liver in obstructive jaundice]. The conducted clinico-experimental study (an investigation of the functional and morphological state of the liver in impaired bile outflow) indicated different compensatory reactions. It is the authors' opinion that bile congestion in the liver is accompanied with proliferation of bile ducts; a damaging effect of bile components, in the first place bilious acids, is manifested in pericholangitis, periductal fibrosis and later in the development of biliary cirrhosis; alterations of the parenchyma occur secondarily also as a result of the toxic effect of bilous components and show a focal character.", "PMID": 960492}
{"id": "pubmed23n0032_3956", "title": "[Perforation of hydatid cysts of the liver into the abdominal cavity].", "content": "Perforation of hydatid hepatic cysts in the abdominal cavity was observed in 35 of 231 patients (14.7%). In its typical course the following periods in the clinical picture may be distinguished: anaphylactic shock, a period of subsidence, peritonitis. Asymptomatic perforation can result in the development of \"echinococcic pseudotuberculosis of the peritoneum\" with a favourable course or dissemination of the peritoneum with subsequent development a progressive ascites, cachexy and anemia.", "contents": "[Perforation of hydatid cysts of the liver into the abdominal cavity]. Perforation of hydatid hepatic cysts in the abdominal cavity was observed in 35 of 231 patients (14.7%). In its typical course the following periods in the clinical picture may be distinguished: anaphylactic shock, a period of subsidence, peritonitis. Asymptomatic perforation can result in the development of \"echinococcic pseudotuberculosis of the peritoneum\" with a favourable course or dissemination of the peritoneum with subsequent development a progressive ascites, cachexy and anemia.", "PMID": 960493}
{"id": "pubmed23n0032_3957", "title": "[Volemic indicators in patients with acute cholecystitis].", "content": "The work is concerned with changes in extracellular fluid and its ingradient--interstitial volume, in particular, as well as TCB and its components influenced by an operative intervention, performed in patients over 50 years of age for acute cholecystitis. Based on the data obtained, it is concluded that in the early postoperative period along with parenteral injection of fluids also it is essential to correct the deficiency of TCB and its components by administration of high-molecular compounds, protein substances, plasma.", "contents": "[Volemic indicators in patients with acute cholecystitis]. The work is concerned with changes in extracellular fluid and its ingradient--interstitial volume, in particular, as well as TCB and its components influenced by an operative intervention, performed in patients over 50 years of age for acute cholecystitis. Based on the data obtained, it is concluded that in the early postoperative period along with parenteral injection of fluids also it is essential to correct the deficiency of TCB and its components by administration of high-molecular compounds, protein substances, plasma.", "PMID": 960494}
{"id": "pubmed23n0032_3958", "title": "[Roentgenocontrast study of pancreatic ducts in surgery of the pancreatic-biliary system].", "content": "The authors report the data obtained as a result of exploration of the main pancreatic duct pre- and intraoperatively. The study was carried out in 34 patients with pancreatic fistulas. Endoscopic catheterization of the papilla duodeni was undertaken in 80 patients, it proved to be a success in 71. Pancreaticograms was made in 63 observations. Contrasting of the duct of Wirsung was noted in 167 of 263 patients in whom operative cholangiography was performed. The data obtained indicated that pre- and intraoperative pancreaticography is a valuable method of investigation, which aids in determining surgical policy in chronic pancreatic lesions and in formulating rational grounds for direct operative interventions on the pancreas.", "contents": "[Roentgenocontrast study of pancreatic ducts in surgery of the pancreatic-biliary system]. The authors report the data obtained as a result of exploration of the main pancreatic duct pre- and intraoperatively. The study was carried out in 34 patients with pancreatic fistulas. Endoscopic catheterization of the papilla duodeni was undertaken in 80 patients, it proved to be a success in 71. Pancreaticograms was made in 63 observations. Contrasting of the duct of Wirsung was noted in 167 of 263 patients in whom operative cholangiography was performed. The data obtained indicated that pre- and intraoperative pancreaticography is a valuable method of investigation, which aids in determining surgical policy in chronic pancreatic lesions and in formulating rational grounds for direct operative interventions on the pancreas.", "PMID": 960495}
{"id": "pubmed23n0032_3959", "title": "[Disorder of regional mesenteric circulation as a cause of functional intestinal obstruction].", "content": "It is shown experimentally that an impaired mesenterial blood circulation is of primary importance for development of dynamic intestinal obstruction, that is in contrast with the existing opinion on the primary importance of disorders in the bowel motility. The data obtained were utilized to explain the mechanisms of a stimulating effect of different procedures in combating against dynamic intestinal obstruction, namely an elimination of the suppressive sympathetic effect on abdominal vessels and an increase of the volume blood flow.", "contents": "[Disorder of regional mesenteric circulation as a cause of functional intestinal obstruction]. It is shown experimentally that an impaired mesenterial blood circulation is of primary importance for development of dynamic intestinal obstruction, that is in contrast with the existing opinion on the primary importance of disorders in the bowel motility. The data obtained were utilized to explain the mechanisms of a stimulating effect of different procedures in combating against dynamic intestinal obstruction, namely an elimination of the suppressive sympathetic effect on abdominal vessels and an increase of the volume blood flow.", "PMID": 960496}
{"id": "pubmed23n0032_3960", "title": "[Sphincter-preserving operations in rectal cancer].", "content": "Among 418 rectal operations for cancer in 63 cases abdomino-anal resection (amputation) of the rectum was performed with descending of the colon, if indicated. The following postoperative complications were observed: necrosis of the descended colon, scarring strictures, various urinary disturbances, peritonitis. The postoperative mortality made 3 per cent.", "contents": "[Sphincter-preserving operations in rectal cancer]. Among 418 rectal operations for cancer in 63 cases abdomino-anal resection (amputation) of the rectum was performed with descending of the colon, if indicated. The following postoperative complications were observed: necrosis of the descended colon, scarring strictures, various urinary disturbances, peritonitis. The postoperative mortality made 3 per cent.", "PMID": 960497}
{"id": "pubmed23n0032_3961", "title": "[Rationale for the right-side puncture of the subclavian vein in patients undergoing heart surgery].", "content": "Based on the study of the literature data and personal author's clinical material (682 punctures) the author was able to reveal the advantages of repeated left-sided puncture of the subclavian vein. An attempt is made to systematize complications relative to this procedure, depending on the side of puncturing in cardiosurgical patients.", "contents": "[Rationale for the right-side puncture of the subclavian vein in patients undergoing heart surgery]. Based on the study of the literature data and personal author's clinical material (682 punctures) the author was able to reveal the advantages of repeated left-sided puncture of the subclavian vein. An attempt is made to systematize complications relative to this procedure, depending on the side of puncturing in cardiosurgical patients.", "PMID": 960498}
{"id": "pubmed23n0032_3962", "title": "[Pathophysiological and anatomical prerequisites of hemodynamic disorders in the system of small saphenous vein].", "content": "112 patients with varicose small sephenous vein were operated upon. The operation was preceded by a careful clinical investigation, including phlebography. The observed changes were correlated with operative findings. It is shown that varicosity may be conditioned by an obstruction of blood outflow in the popliteal vein or small saphenous vein ostium. A relationship between some structural variants of the small saphenous vein and hemodynamic disturbances was noted.", "contents": "[Pathophysiological and anatomical prerequisites of hemodynamic disorders in the system of small saphenous vein]. 112 patients with varicose small sephenous vein were operated upon. The operation was preceded by a careful clinical investigation, including phlebography. The observed changes were correlated with operative findings. It is shown that varicosity may be conditioned by an obstruction of blood outflow in the popliteal vein or small saphenous vein ostium. A relationship between some structural variants of the small saphenous vein and hemodynamic disturbances was noted.", "PMID": 960499}
{"id": "pubmed23n0032_3963", "title": "[Dynamic pressure in the superficial and deep crural veins in uncomplicated varicosity without incompetence of communicant veins].", "content": "Synchronous phlebotonometry was performed on 30 lower extremities in 27 patients. Various pressure dynamics was observed in the proximal and distal segments of profound veins. Dynamic phlebohypertension was found not only in superficial but also in deep-seated veins. The escape of superficial veins resulted in normalization of pressure in both systems. The more intensive muscle contractions under conditions of normal blood circulation the greater a reduction of pressure phases in the crural vein distal segment this factor may be used as a test for estimation of the vein-muscle pump function.", "contents": "[Dynamic pressure in the superficial and deep crural veins in uncomplicated varicosity without incompetence of communicant veins]. Synchronous phlebotonometry was performed on 30 lower extremities in 27 patients. Various pressure dynamics was observed in the proximal and distal segments of profound veins. Dynamic phlebohypertension was found not only in superficial but also in deep-seated veins. The escape of superficial veins resulted in normalization of pressure in both systems. The more intensive muscle contractions under conditions of normal blood circulation the greater a reduction of pressure phases in the crural vein distal segment this factor may be used as a test for estimation of the vein-muscle pump function.", "PMID": 960500}
{"id": "pubmed23n0032_3964", "title": "[Complications in surgical treatment of varicose veins of the lower limbs and their prevention].", "content": "To prevent hemorrhage and hematomas in the bed of subcutaneously removed large saphenous vein after Babcock the authors somewhat modified tactically the order of manipulations during the operative procedure. This method was employed while operating 42 patients with primary and secondary venous varices. It was feasible to prevent hemorrhage and hematomas along the vascular bed in every case. The suggested tactical modification of the removal of venous varices wound improve the postoperative course and shorten the terms of patients' stay at the hospital postoperatively.", "contents": "[Complications in surgical treatment of varicose veins of the lower limbs and their prevention]. To prevent hemorrhage and hematomas in the bed of subcutaneously removed large saphenous vein after Babcock the authors somewhat modified tactically the order of manipulations during the operative procedure. This method was employed while operating 42 patients with primary and secondary venous varices. It was feasible to prevent hemorrhage and hematomas along the vascular bed in every case. The suggested tactical modification of the removal of venous varices wound improve the postoperative course and shorten the terms of patients' stay at the hospital postoperatively.", "PMID": 960501}
{"id": "pubmed23n0032_3965", "title": "[Bilateral coxarthroses and avascular necrosis of the femur head in adults].", "content": "Based on the authors' personal experience, it was concluded that in surgical treatment for coxarthrosis intertrochanteric osteotomy is to be preferred, since it proved to be effective even in advanced cases of the lesion in the extent of residual motions of 30--40degrees. If indicated, the procedure may be bilateral. The operation yielded the best results also in avascular articular trophesy with resorption of sequestrae. In grave cases it is rational to begin with prosthesizing of one of the joints.", "contents": "[Bilateral coxarthroses and avascular necrosis of the femur head in adults]. Based on the authors' personal experience, it was concluded that in surgical treatment for coxarthrosis intertrochanteric osteotomy is to be preferred, since it proved to be effective even in advanced cases of the lesion in the extent of residual motions of 30--40degrees. If indicated, the procedure may be bilateral. The operation yielded the best results also in avascular articular trophesy with resorption of sequestrae. In grave cases it is rational to begin with prosthesizing of one of the joints.", "PMID": 960502}
{"id": "pubmed23n0032_3966", "title": "[Alkaline phosphatase of granulocytes in open fractures of the long tubular bones].", "content": "Investigations carried out in 60 patients with open fractures of long tubular bones in osteosynthesis with the Ilizarov apparatus indicated that alkaline phosphatase (AP) of granulocytes fully reflects the postoperative course. 10--24 hours following the trauma the level of AP in patients with grave injuries exceeds that in patients with slight ones. The differences are found to be reliable from the 1st to the 5th day after the injury.", "contents": "[Alkaline phosphatase of granulocytes in open fractures of the long tubular bones]. Investigations carried out in 60 patients with open fractures of long tubular bones in osteosynthesis with the Ilizarov apparatus indicated that alkaline phosphatase (AP) of granulocytes fully reflects the postoperative course. 10--24 hours following the trauma the level of AP in patients with grave injuries exceeds that in patients with slight ones. The differences are found to be reliable from the 1st to the 5th day after the injury.", "PMID": 960503}
{"id": "pubmed23n0032_3967", "title": "[Surgical tactics in severe combined injuries of the face and other body regions].", "content": "The results of treatment in 67 patients with associated injuries of the face and other regions of the body are analysed. Sixteen of them died. Their poor state was conditioned not by facial injuries but by traumas of other organs and system of the organism. It is concluded that such patients should be treated not in stomatological but in special traumatological departments for polytraumas. Surgery is first to be performed in the region where the damage is most hazardous. But the first step is to prevent aspiration of blood from the oral cavity into the respiratory tract followed by the fixation of jaw fragments.", "contents": "[Surgical tactics in severe combined injuries of the face and other body regions]. The results of treatment in 67 patients with associated injuries of the face and other regions of the body are analysed. Sixteen of them died. Their poor state was conditioned not by facial injuries but by traumas of other organs and system of the organism. It is concluded that such patients should be treated not in stomatological but in special traumatological departments for polytraumas. Surgery is first to be performed in the region where the damage is most hazardous. But the first step is to prevent aspiration of blood from the oral cavity into the respiratory tract followed by the fixation of jaw fragments.", "PMID": 960504}
{"id": "pubmed23n0032_3968", "title": "[Prevention and treatment of staphylococcal infections and their complications, in surgical practice with biological products].", "content": "In the clinic 307 patients with suppurative lesions were observed, in 183 of them (the main group) staphylococcic anatoxin was administered with prophylactic and therapeutic purposes, 124 patients (the control group) were treated with antibiotics, including 231 patients with acute appendicitis, 23--with osteomyelitis, 48--with mastitis and 5 patients with various purulent complications of staphylococcic etiology. Among 231 patients with acute appendicitis 125 comprised the main group who were injected staphylococcic anatoxin, and 106--the control group. In the latter the incidence of the operative wound suppuration was observed thrice as frequently.", "contents": "[Prevention and treatment of staphylococcal infections and their complications, in surgical practice with biological products]. In the clinic 307 patients with suppurative lesions were observed, in 183 of them (the main group) staphylococcic anatoxin was administered with prophylactic and therapeutic purposes, 124 patients (the control group) were treated with antibiotics, including 231 patients with acute appendicitis, 23--with osteomyelitis, 48--with mastitis and 5 patients with various purulent complications of staphylococcic etiology. Among 231 patients with acute appendicitis 125 comprised the main group who were injected staphylococcic anatoxin, and 106--the control group. In the latter the incidence of the operative wound suppuration was observed thrice as frequently.", "PMID": 960505}
{"id": "pubmed23n0032_3969", "title": "[Compression-distraction osteosynthesis in pseudarthroses of long tubular bones].", "content": "An experience of the clinic with treatment of pseudarthrosis of tubular bones in 163 patients, using compression-distraction osteosynthesis is reported. In their anatomical localization pseudarthroses were distributed as follows: the femur-22, the crus-112, the shoulder-22, the forearm-7. The Ilizarov and Gudushauri compression apparatus was employed in 143 patients, compression osteosynthesis after Greifensteiner was performed in 20 patients. The results of treatment were studied in 155 patients within the terms from 10 months to 5 years. Good issues were obtained in 95 (61.3%) patients, satisfactory- in 45 (29%), poor--in 15 (9.7%).", "contents": "[Compression-distraction osteosynthesis in pseudarthroses of long tubular bones]. An experience of the clinic with treatment of pseudarthrosis of tubular bones in 163 patients, using compression-distraction osteosynthesis is reported. In their anatomical localization pseudarthroses were distributed as follows: the femur-22, the crus-112, the shoulder-22, the forearm-7. The Ilizarov and Gudushauri compression apparatus was employed in 143 patients, compression osteosynthesis after Greifensteiner was performed in 20 patients. The results of treatment were studied in 155 patients within the terms from 10 months to 5 years. Good issues were obtained in 95 (61.3%) patients, satisfactory- in 45 (29%), poor--in 15 (9.7%).", "PMID": 960506}
{"id": "pubmed23n0032_3970", "title": "[Remote results of treatment of compression fractures of the calcaneus].", "content": "Late results of treatment in fractures of the calcanean bone were studied in 72 patients within the terms from 2 to 11 years. In 41 of these cases a grave compression fracture of the calcanean bone was observed, in 9--there was a damage of both calcanean bones. 37 patients were treated clinically and 4--under outpatient conditions. Skeletal extension was used in 12 patients during 10--25 days, in 3--it was complicated with suppuration. Manual reposition was employed in 19 cases, and it was not used in 6. It is the author's opinion that permanent and one-moment skeletal extension by multiple pins inserted under general aneshesia and roentgeno-telecontrol should be preferred.", "contents": "[Remote results of treatment of compression fractures of the calcaneus]. Late results of treatment in fractures of the calcanean bone were studied in 72 patients within the terms from 2 to 11 years. In 41 of these cases a grave compression fracture of the calcanean bone was observed, in 9--there was a damage of both calcanean bones. 37 patients were treated clinically and 4--under outpatient conditions. Skeletal extension was used in 12 patients during 10--25 days, in 3--it was complicated with suppuration. Manual reposition was employed in 19 cases, and it was not used in 6. It is the author's opinion that permanent and one-moment skeletal extension by multiple pins inserted under general aneshesia and roentgeno-telecontrol should be preferred.", "PMID": 960507}
{"id": "pubmed23n0032_3971", "title": "Combined immunodeficiency with failure of colostral immunoglobulins transfer in foals.", "content": "Foals with combined immunodeficiency (CID), a fatal genetic defect in the production of both B and T lymphocytes, are born without immunoglobulins and are unable to synthesise them. CID foals receiving immunoglobulins via the dam's colostrum may live up to four months of age. Those CID foals with failure of passive transfer (FPT) die at a much earlier age. The occurrence of CID is of value in studying passive transfer of immunoglobulins, as no confusion exists as to when passive transfer ends and active synthesis of immunoglobulins begins. A high correlation has been found between early foal disease and deaths and lack of passive transfer of immunoglobulins, even though many of these foals appear to nurse normally during the first few hours post partum. Evaluation of immunoglobulin levels in 24 hour post suckle samples would prove of value not only in diagnosing CID foals, but in recognising FPT in otherwise normal foals.", "contents": "Combined immunodeficiency with failure of colostral immunoglobulins transfer in foals. Foals with combined immunodeficiency (CID), a fatal genetic defect in the production of both B and T lymphocytes, are born without immunoglobulins and are unable to synthesise them. CID foals receiving immunoglobulins via the dam's colostrum may live up to four months of age. Those CID foals with failure of passive transfer (FPT) die at a much earlier age. The occurrence of CID is of value in studying passive transfer of immunoglobulins, as no confusion exists as to when passive transfer ends and active synthesis of immunoglobulins begins. A high correlation has been found between early foal disease and deaths and lack of passive transfer of immunoglobulins, even though many of these foals appear to nurse normally during the first few hours post partum. Evaluation of immunoglobulin levels in 24 hour post suckle samples would prove of value not only in diagnosing CID foals, but in recognising FPT in otherwise normal foals.", "PMID": 960512}
{"id": "pubmed23n0032_3972", "title": "Retention of the fetal membranes in cattle.", "content": "An investigation of the possible significance of 'stress' as a factor in the retention of bovine fetal membranes has been carried out, together with the investigation as to the possible variation of progesterone levels at parturition, as a cause of retention. No evidence of abnormal progesterone levels has been established, but from the evidence it is concluded that retention may well be yet another example of production disease.", "contents": "Retention of the fetal membranes in cattle. An investigation of the possible significance of 'stress' as a factor in the retention of bovine fetal membranes has been carried out, together with the investigation as to the possible variation of progesterone levels at parturition, as a cause of retention. No evidence of abnormal progesterone levels has been established, but from the evidence it is concluded that retention may well be yet another example of production disease.", "PMID": 960513}
{"id": "pubmed23n0032_3973", "title": "The selection of antibiotics.", "content": "The usefulness of an antibiotic depends not only upon its antibacterial potency and spectrum but also on the prevalence of resistant organisms and the extent and severity of the adverse reactions to which it may give rise. Variations in formulation of the same compound are reflected in differences in bioavailability. These may be intentional, as in the development of long-acting preparations, but may also be unexpected following differences in drug purity, content and gastro-intestinal absorption. Individual and species differences in treated animals also result in variations in bioavailability. The merits of combination products of two or more antibiotics are often equivocal and some combinations are definitely disadvantageous. Practical rules for the selection and use of antibiotics emphasise the need for full doses of effective compounds used for limited periods with monitoring of patterns of sensitivity so that problems of large-scale resistance can be avoided.", "contents": "The selection of antibiotics. The usefulness of an antibiotic depends not only upon its antibacterial potency and spectrum but also on the prevalence of resistant organisms and the extent and severity of the adverse reactions to which it may give rise. Variations in formulation of the same compound are reflected in differences in bioavailability. These may be intentional, as in the development of long-acting preparations, but may also be unexpected following differences in drug purity, content and gastro-intestinal absorption. Individual and species differences in treated animals also result in variations in bioavailability. The merits of combination products of two or more antibiotics are often equivocal and some combinations are definitely disadvantageous. Practical rules for the selection and use of antibiotics emphasise the need for full doses of effective compounds used for limited periods with monitoring of patterns of sensitivity so that problems of large-scale resistance can be avoided.", "PMID": 960518}
{"id": "pubmed23n0032_3974", "title": "Design and farm animal welfare.", "content": "Farm animal welfare and the design of farm buildings and equipment are interrelated. The animals' requirements and preferences should first be estimated and ways in which this can be done are discussed, as are methods of assessment of their environment. Some examples of the influence which housing and equipment design can have are given. Attention is drawn to the difficulties inherent in the assessment of farm animal welfare and the postulation made that the veterinarian is well fitted to carry out such assessments.", "contents": "Design and farm animal welfare. Farm animal welfare and the design of farm buildings and equipment are interrelated. The animals' requirements and preferences should first be estimated and ways in which this can be done are discussed, as are methods of assessment of their environment. Some examples of the influence which housing and equipment design can have are given. Attention is drawn to the difficulties inherent in the assessment of farm animal welfare and the postulation made that the veterinarian is well fitted to carry out such assessments.", "PMID": 960519}
{"id": "pubmed23n0032_3975", "title": "Equine artificial insemination.", "content": "The use and techniques of artificial insemination for horses in Germany over the last 30 years is described. Artificial insemination appears to produce pregnancy percentages equal to those from normal breeding methods and its continued availability under veterinary supervision is recommended in conditions where disease, disability or distance debar normal service.", "contents": "Equine artificial insemination. The use and techniques of artificial insemination for horses in Germany over the last 30 years is described. Artificial insemination appears to produce pregnancy percentages equal to those from normal breeding methods and its continued availability under veterinary supervision is recommended in conditions where disease, disability or distance debar normal service.", "PMID": 960520}
{"id": "pubmed23n0032_3976", "title": "[Method for computing the milk losses from sterility in cows].", "content": "An attempt was made to work out a method and establish the indices in calculating the losses of milk due to sterility in cows, along with recording the level of productivity and the duration of the period of sterility. The differences were established between: 1. The size of the average daily milk yield per normative day (a day of the period between calvings of 365 days) and that per day of the actual period between calvings. 2. The average daily milk yield per normative day and that per day of the period of sterility. The milk losses calculated in both ways gave almost comparable results. It is , therefore, suggested when calculating the losses due to sterility to use the established indices of milk losses per sterility day (Table 2) at a varying milkiness of the cows and a varying length of the period between calvings, resp., the period of sterility.", "contents": "[Method for computing the milk losses from sterility in cows]. An attempt was made to work out a method and establish the indices in calculating the losses of milk due to sterility in cows, along with recording the level of productivity and the duration of the period of sterility. The differences were established between: 1. The size of the average daily milk yield per normative day (a day of the period between calvings of 365 days) and that per day of the actual period between calvings. 2. The average daily milk yield per normative day and that per day of the period of sterility. The milk losses calculated in both ways gave almost comparable results. It is , therefore, suggested when calculating the losses due to sterility to use the established indices of milk losses per sterility day (Table 2) at a varying milkiness of the cows and a varying length of the period between calvings, resp., the period of sterility.", "PMID": 960543}
{"id": "pubmed23n0032_3977", "title": "[Epizootiological and parasitological status of the flocks at a poultry combine].", "content": "A comprehensive epizootiologic study has been carried out at one of the large poultry dressing combines in this country. Results have shown that the part played by infections as against the remaining diseases established in the various technologic categories is as follows: 9 to 23.8 per cent in growing parents; 16.0 to 24.6 per cent in adult parents; and 4.8 to 13.4 per cent in broilers. So far as parasitic diseases are concerned the respective figures are 3.2 to 9.9 per cent, 3.0 to 3.3. per cent, and 2.9 to 3.0 to per cent. The import of birds from France and England intended for satisfying the needs of the poultry dressing combine in Rouss\u00e9 has contributed to the introduction of infections which have later on assumed an epizootic course, such as infections encephalomyelitis, infectious bronchitis, and Marek' disease. The epizootic status has been aggravated with the development of a number of diseases already existing in this country--fowl pox, lymphoid leukosis, chronic respiratory disease, salmonellosis, and above all infections caused by the so-called occasionally pathogenic agents--coli organisms, staphylococci, and Pseudomonas. Of the parasitic diseases only coccidiosis has developed, being caused by a variety of species: Eimeria acervulina, Eimeria necatrix, Eimeria maxima, Eimeria muvati and Eimeria tenella. The absence of helminth infections is due to the routinely adopted technological practice of raising birds in confinement and the strict veterinary control and guard. On the basis of the results obtained and the conclusions drawn some more effective prophylactic measures are suggested correcting and supplementing the existing prophylactic programme.", "contents": "[Epizootiological and parasitological status of the flocks at a poultry combine]. A comprehensive epizootiologic study has been carried out at one of the large poultry dressing combines in this country. Results have shown that the part played by infections as against the remaining diseases established in the various technologic categories is as follows: 9 to 23.8 per cent in growing parents; 16.0 to 24.6 per cent in adult parents; and 4.8 to 13.4 per cent in broilers. So far as parasitic diseases are concerned the respective figures are 3.2 to 9.9 per cent, 3.0 to 3.3. per cent, and 2.9 to 3.0 to per cent. The import of birds from France and England intended for satisfying the needs of the poultry dressing combine in Rouss\u00e9 has contributed to the introduction of infections which have later on assumed an epizootic course, such as infections encephalomyelitis, infectious bronchitis, and Marek' disease. The epizootic status has been aggravated with the development of a number of diseases already existing in this country--fowl pox, lymphoid leukosis, chronic respiratory disease, salmonellosis, and above all infections caused by the so-called occasionally pathogenic agents--coli organisms, staphylococci, and Pseudomonas. Of the parasitic diseases only coccidiosis has developed, being caused by a variety of species: Eimeria acervulina, Eimeria necatrix, Eimeria maxima, Eimeria muvati and Eimeria tenella. The absence of helminth infections is due to the routinely adopted technological practice of raising birds in confinement and the strict veterinary control and guard. On the basis of the results obtained and the conclusions drawn some more effective prophylactic measures are suggested correcting and supplementing the existing prophylactic programme.", "PMID": 960544}
{"id": "pubmed23n0032_3978", "title": "[Changes in the total protein and protein fractions in cow's milk irradiated with ultraviolet rays].", "content": "Comparative experimental electrophoretic studies were carried out to establish the effect of ultraviolet treatment on cow's milk at various rates of irradiation (5, 10 and 15 min) with a view to raising and regulating the content of the total protein and the protein fractions in milk intended for the feeding of calves. Results showed that there were no substantial changes in the level of the total protein, the albumins having a rising trend that was manifested best at a 15-minute treatment. Most characteristic were the changes taking place in the immunoglobulin fraction which rose by 4.7 per cent. The beta-lactoglobulin fraction dropped by 4.5 per cent, on an average, at all rates of treatment, and the level of alfa-lactoglobulin at 5- and 10-minute irradiation underwent almost no changes, it rising by about 2.09 per cent, on an average, at 15-min treatment. Similar was the trend manifested by the proteoso-pepton fraction showing a rise as early as the first ten minutes of treatment.", "contents": "[Changes in the total protein and protein fractions in cow's milk irradiated with ultraviolet rays]. Comparative experimental electrophoretic studies were carried out to establish the effect of ultraviolet treatment on cow's milk at various rates of irradiation (5, 10 and 15 min) with a view to raising and regulating the content of the total protein and the protein fractions in milk intended for the feeding of calves. Results showed that there were no substantial changes in the level of the total protein, the albumins having a rising trend that was manifested best at a 15-minute treatment. Most characteristic were the changes taking place in the immunoglobulin fraction which rose by 4.7 per cent. The beta-lactoglobulin fraction dropped by 4.5 per cent, on an average, at all rates of treatment, and the level of alfa-lactoglobulin at 5- and 10-minute irradiation underwent almost no changes, it rising by about 2.09 per cent, on an average, at 15-min treatment. Similar was the trend manifested by the proteoso-pepton fraction showing a rise as early as the first ten minutes of treatment.", "PMID": 960545}
{"id": "pubmed23n0032_3979", "title": "[Effectiveness of Deptavac HVT vaccine on broiler parents during the egg-laying period].", "content": "Experiments were carried out with 4 flocks-a total of 16,940 layers of the White Plymouth Rock breed (10,865 vaccinated and 6075 untreated) and 3000 cocks of the White Cornish breed (502 vaccinated and 1498 untreated). The test birds had been vaccinated at the age of one day. The average month mortality rate among the vaccinated birds was 1.5 per cent, and among the untreated it was 4.9 per cent; for the whole exploitation period these values were 15.35 and 47.17 per cent, respectively. Mortality rate caused by Marek's disease and lymphoid leukosis (specific mortality) in the test groups was 4 times lower as compared with that of the control groups (11.54 as against 44.75 per cent), and the coefficient of epizootiologic effectiveness was 74.4 per cent. Considerable variation was established in the intensity and dynamics of the epizootic process with the vaccinated and unvaccinated birds, on the one hand, and the layers and cocks, on the other. White Cornish cocks proved more resistant to Marek's disease and more sensitive to lymphoid leukosis, while the layers of the White Plymouth Rock breed were more sensitive to Marek's disease and more resistant to lymphoid leukosis. These differences could explain the dynamics peculiarity of the specific mortality exhibited by layers and cocks. The egg-laying capacity of a \"forage\" layer up to the age of 62 weeks of age in the test flocks was 9.2 (7.2) per cent higher than that of the untreated layers. In exploitation up to the 70th week of age each test layer gave 23 eggs more as compared to the untreated. It is calculated on the basis of the experimental data that a total of 55,200 eggs and more could be obtained from 6000 parental birds vaccinated against Marek's disease during a 10-month laying period.", "contents": "[Effectiveness of Deptavac HVT vaccine on broiler parents during the egg-laying period]. Experiments were carried out with 4 flocks-a total of 16,940 layers of the White Plymouth Rock breed (10,865 vaccinated and 6075 untreated) and 3000 cocks of the White Cornish breed (502 vaccinated and 1498 untreated). The test birds had been vaccinated at the age of one day. The average month mortality rate among the vaccinated birds was 1.5 per cent, and among the untreated it was 4.9 per cent; for the whole exploitation period these values were 15.35 and 47.17 per cent, respectively. Mortality rate caused by Marek's disease and lymphoid leukosis (specific mortality) in the test groups was 4 times lower as compared with that of the control groups (11.54 as against 44.75 per cent), and the coefficient of epizootiologic effectiveness was 74.4 per cent. Considerable variation was established in the intensity and dynamics of the epizootic process with the vaccinated and unvaccinated birds, on the one hand, and the layers and cocks, on the other. White Cornish cocks proved more resistant to Marek's disease and more sensitive to lymphoid leukosis, while the layers of the White Plymouth Rock breed were more sensitive to Marek's disease and more resistant to lymphoid leukosis. These differences could explain the dynamics peculiarity of the specific mortality exhibited by layers and cocks. The egg-laying capacity of a \"forage\" layer up to the age of 62 weeks of age in the test flocks was 9.2 (7.2) per cent higher than that of the untreated layers. In exploitation up to the 70th week of age each test layer gave 23 eggs more as compared to the untreated. It is calculated on the basis of the experimental data that a total of 55,200 eggs and more could be obtained from 6000 parental birds vaccinated against Marek's disease during a 10-month laying period.", "PMID": 960546}
{"id": "pubmed23n0032_3980", "title": "[Characteristics of the staphylococci isolated from the udder of cows with mastitis].", "content": "A total of 175 strains of Staphylococcus aureus and 67 strains of Staphylococcus epidermidis were studied, isolated from 486 samples of milk secretion taken aceptically from the individual quarters of the udder of cows affected with subclinical and purulent (clinical) mastitis. The staphylococci were referred to as the causative agent of mastitis in case they were the only microflora in the seedings of the investigated material. Tests were applied as given in Fig. 1 to characterize the strains. It was found that mastitis in cows could be due to both plasma coagulating staphylococci (Staphylococcus aureus) and coagulase-negative Staphylococcus epidermidis organisms. The two Staphylococcus species were isolated from cows with clinical and subclinical mastitis. The division between pathogenic and nonpathogenic Staphylococcus strains by the plasma-coagulating symptom proved impossible, and this made it necessary to use other tests for pathogenicity. It became evident that the thing Staph. aureus and Staph. epidermidis had in common when isolated from cows with mastitis was the production of a gold-like pigment and delta hemolysin. Similarly to Staph. aureus isolated animals, the bovine Staph. epidermidis organisms did not possess fibrinolysin and rarely produced hemolysin. The isolated organisms belonging to the coagulase-positive staphylococci corresponded by their basic properties to Staphylococcus aureus var. bovis as described in the literature. The cultures of Staphylococcus epidermidis isolated under similar conditions showed in a considerable per cent of the cases somewhat different behaviour.", "contents": "[Characteristics of the staphylococci isolated from the udder of cows with mastitis]. A total of 175 strains of Staphylococcus aureus and 67 strains of Staphylococcus epidermidis were studied, isolated from 486 samples of milk secretion taken aceptically from the individual quarters of the udder of cows affected with subclinical and purulent (clinical) mastitis. The staphylococci were referred to as the causative agent of mastitis in case they were the only microflora in the seedings of the investigated material. Tests were applied as given in Fig. 1 to characterize the strains. It was found that mastitis in cows could be due to both plasma coagulating staphylococci (Staphylococcus aureus) and coagulase-negative Staphylococcus epidermidis organisms. The two Staphylococcus species were isolated from cows with clinical and subclinical mastitis. The division between pathogenic and nonpathogenic Staphylococcus strains by the plasma-coagulating symptom proved impossible, and this made it necessary to use other tests for pathogenicity. It became evident that the thing Staph. aureus and Staph. epidermidis had in common when isolated from cows with mastitis was the production of a gold-like pigment and delta hemolysin. Similarly to Staph. aureus isolated animals, the bovine Staph. epidermidis organisms did not possess fibrinolysin and rarely produced hemolysin. The isolated organisms belonging to the coagulase-positive staphylococci corresponded by their basic properties to Staphylococcus aureus var. bovis as described in the literature. The cultures of Staphylococcus epidermidis isolated under similar conditions showed in a considerable per cent of the cases somewhat different behaviour.", "PMID": 960547}
{"id": "pubmed23n0032_3981", "title": "[Comparative studies of methods for the diagnosis of Aujeszkh's disease].", "content": "The virologic studies of a total of 383 organs taken from 183 pigs using a rabbit as a test animal and cell cultures of chick embryo fibroblasts and pig kidney revealed that the per cent of the positive results obtained by the three methods was varying with regard both to the organs and the various categories of animal used. The biologic test proved appropriate in the cases of acute diseases, while with diseases with atypical and symptomless course the cell culture methods are to be used, most suitable for the mass laboratory diagnosis being the chick embryo fibroblasts. The successful diagnosis requires a complex study method with the use of greater number of viscera.", "contents": "[Comparative studies of methods for the diagnosis of Aujeszkh's disease]. The virologic studies of a total of 383 organs taken from 183 pigs using a rabbit as a test animal and cell cultures of chick embryo fibroblasts and pig kidney revealed that the per cent of the positive results obtained by the three methods was varying with regard both to the organs and the various categories of animal used. The biologic test proved appropriate in the cases of acute diseases, while with diseases with atypical and symptomless course the cell culture methods are to be used, most suitable for the mass laboratory diagnosis being the chick embryo fibroblasts. The successful diagnosis requires a complex study method with the use of greater number of viscera.", "PMID": 960548}
{"id": "pubmed23n0032_3982", "title": "[Study of Mycoplasma from the genital apparatus of cattle].", "content": "The study on vaginal mucous secretion in cows with metritis and vaginitis, on fetuses and placentae of cows that had miscarried as well as on preputial secretion of bulls revealed the presence of Mycoplasma organisms associated with V. fetus and other bacterial species. By their reaction to cholesterol, digitonin, sodium polyanetol sulfonate as well as their serum and temperature requirements, the formation of films and spots, their phosphatase activity and biochemical and serologic behaviour the mycoplasmas isolated from the genital tract of cows were specified as A. laidlawii and A. axanthum. From both cows and bulls T-forms of mycoplasmas were isolated. The strains determined as A. laidlawi showed deviations from the species characteristics by the fermentation of glucose, hydrolysis of esculine, and reduction of 2,3,5-triphenyl-tetrazolium chloride.", "contents": "[Study of Mycoplasma from the genital apparatus of cattle]. The study on vaginal mucous secretion in cows with metritis and vaginitis, on fetuses and placentae of cows that had miscarried as well as on preputial secretion of bulls revealed the presence of Mycoplasma organisms associated with V. fetus and other bacterial species. By their reaction to cholesterol, digitonin, sodium polyanetol sulfonate as well as their serum and temperature requirements, the formation of films and spots, their phosphatase activity and biochemical and serologic behaviour the mycoplasmas isolated from the genital tract of cows were specified as A. laidlawii and A. axanthum. From both cows and bulls T-forms of mycoplasmas were isolated. The strains determined as A. laidlawi showed deviations from the species characteristics by the fermentation of glucose, hydrolysis of esculine, and reduction of 2,3,5-triphenyl-tetrazolium chloride.", "PMID": 960549}
{"id": "pubmed23n0032_3983", "title": "[Effect of different amounts of carbamide on nitrogen digestibility and metabolism in broilers. II].", "content": "Experiments were carried out with one-hundred broiler birds of one and the same age group, breed, and origin, divided into five groups in order to follow up the effect of various amounts of urea on their physiologic state. A balance experiment was conducted to establish the effect of urea on the digestibility of nutrient matter and the metabolism of nitrogen with the use of nine birds per group, divided into three subgroups. Urea was added to a protein-balanced diet from the 20th to the 60th day in the following amounts by groups: I--2 percent; II--4 per cent; III--5 per cent; and IV--6 per cent. It was also found that with the increase of urea in the ration at the rates indicated above there was a progressive rise of both the amount of urea and the nonprotein nitrogen in the blood as well as the amount of nitrogen excreted with the urine. The intake of such amounts of urea lowered the assimilation of nitrogen by 4 to 9 per cent as against its initial level in the ration, and by 7 to 11 per cent--as against the level of digested nitrogen.", "contents": "[Effect of different amounts of carbamide on nitrogen digestibility and metabolism in broilers. II]. Experiments were carried out with one-hundred broiler birds of one and the same age group, breed, and origin, divided into five groups in order to follow up the effect of various amounts of urea on their physiologic state. A balance experiment was conducted to establish the effect of urea on the digestibility of nutrient matter and the metabolism of nitrogen with the use of nine birds per group, divided into three subgroups. Urea was added to a protein-balanced diet from the 20th to the 60th day in the following amounts by groups: I--2 percent; II--4 per cent; III--5 per cent; and IV--6 per cent. It was also found that with the increase of urea in the ration at the rates indicated above there was a progressive rise of both the amount of urea and the nonprotein nitrogen in the blood as well as the amount of nitrogen excreted with the urine. The intake of such amounts of urea lowered the assimilation of nitrogen by 4 to 9 per cent as against its initial level in the ration, and by 7 to 11 per cent--as against the level of digested nitrogen.", "PMID": 960550}
{"id": "pubmed23n0032_3984", "title": "[Lysogeny in Pasteurella multocida].", "content": "Studied was the possibility of lysis-producing factors (the phenomenon of lysogeny) with 59 strains of Pastuerella multocida isolated in Bulgaria, Cuba, and Czechoslovakia. It was found that eleven of them were lysogenic in terms that a total of 12 bacteriophage strains were isolated from them; one of them yielded 2 phages. Established were three different indicator strains of Pasteurella multocida-97, SHD, and R-II--by means of which 3 different groups of P. multocida phages were isolated. The latter were stabilized and allowed to multiply up to 10(11) phage particles per 1 cc through continuous passaging, and they could be be stored at + 4 degrees C. In accordance with the host strain for multiplying the isolated P. multocida phages were divided into three different groups: phages 3, 4, 5, 6, 22, and Sl fell into group II, and phages 1075 and S-2--to group III.", "contents": "[Lysogeny in Pasteurella multocida]. Studied was the possibility of lysis-producing factors (the phenomenon of lysogeny) with 59 strains of Pastuerella multocida isolated in Bulgaria, Cuba, and Czechoslovakia. It was found that eleven of them were lysogenic in terms that a total of 12 bacteriophage strains were isolated from them; one of them yielded 2 phages. Established were three different indicator strains of Pasteurella multocida-97, SHD, and R-II--by means of which 3 different groups of P. multocida phages were isolated. The latter were stabilized and allowed to multiply up to 10(11) phage particles per 1 cc through continuous passaging, and they could be be stored at + 4 degrees C. In accordance with the host strain for multiplying the isolated P. multocida phages were divided into three different groups: phages 3, 4, 5, 6, 22, and Sl fell into group II, and phages 1075 and S-2--to group III.", "PMID": 960551}
{"id": "pubmed23n0032_3985", "title": "[Study of the pathogenicity and drug sensitivity of staphylococci isolated from poultry].", "content": "Studied were the pathogenicity and drug sensitivity of a total of 64 Staphylococcus strains isolated from chickens and chick embryos. A high per cent value of coincidence was established between the tests of positive plasma-coagulase reaction, hemolysis, golden-yellow pigment production, glucose-positive reaction in anaerobiosis and the lethal sequela following the experimental infection of young chickens and albino mice. The complex use of these tests proved to be a dependable criterion of pathogenicity. Most of the Staphylococcus strains were shown to be strongly and moderately sensitive to kanamycin gallimycin, oximycin, chloramphenicol, and persistent to oxitetracyclin.", "contents": "[Study of the pathogenicity and drug sensitivity of staphylococci isolated from poultry]. Studied were the pathogenicity and drug sensitivity of a total of 64 Staphylococcus strains isolated from chickens and chick embryos. A high per cent value of coincidence was established between the tests of positive plasma-coagulase reaction, hemolysis, golden-yellow pigment production, glucose-positive reaction in anaerobiosis and the lethal sequela following the experimental infection of young chickens and albino mice. The complex use of these tests proved to be a dependable criterion of pathogenicity. Most of the Staphylococcus strains were shown to be strongly and moderately sensitive to kanamycin gallimycin, oximycin, chloramphenicol, and persistent to oxitetracyclin.", "PMID": 960552}
{"id": "pubmed23n0032_3986", "title": "[Biochemical and technological characteristics of the milk from Black-and White Dutch].", "content": "Studied were the composition and the technologic properties of the milk of Dutch Black pied cattle under this country's conditions. Milk samples were taken for analysis once in a month on a cow farm founded in 1964 with a total of 67 impregnated heifers and having at the time of investigation 88 cows, twelve of them remaining from the initial animals imported from Holland and the others being the offspring of the herd. It was established that the annual milk yield per forage cow, amounting to 44881 has the following index values; dry matter--12.63 +/- 0.48 per cent; butterfat per cent--4.07 +/- 0.2%; total protein--3.37 +/- 0.16 per cent; casein--2.56 +/- 0.16; slids-nonfat--8.54 +/- 0.24 per cent; calcium--0.126 +/- 0.002 per cent; phosphorus--0.079 +/- 0.003 per cent. The technologic properties of milk proved unsatisfactory: coagulation capacity--359 s; rheologic value--112.93 X 1.10(-5) V/cm3; and technologic coeficient--1.32. The data on the composition and the technologic properties of Dutch Black-pied cow's milk showed, on the whole, that most profitable is its processing into butter as against processing into cheese in which case the yields would be unsatisfactory.", "contents": "[Biochemical and technological characteristics of the milk from Black-and White Dutch]. Studied were the composition and the technologic properties of the milk of Dutch Black pied cattle under this country's conditions. Milk samples were taken for analysis once in a month on a cow farm founded in 1964 with a total of 67 impregnated heifers and having at the time of investigation 88 cows, twelve of them remaining from the initial animals imported from Holland and the others being the offspring of the herd. It was established that the annual milk yield per forage cow, amounting to 44881 has the following index values; dry matter--12.63 +/- 0.48 per cent; butterfat per cent--4.07 +/- 0.2%; total protein--3.37 +/- 0.16 per cent; casein--2.56 +/- 0.16; slids-nonfat--8.54 +/- 0.24 per cent; calcium--0.126 +/- 0.002 per cent; phosphorus--0.079 +/- 0.003 per cent. The technologic properties of milk proved unsatisfactory: coagulation capacity--359 s; rheologic value--112.93 X 1.10(-5) V/cm3; and technologic coeficient--1.32. The data on the composition and the technologic properties of Dutch Black-pied cow's milk showed, on the whole, that most profitable is its processing into butter as against processing into cheese in which case the yields would be unsatisfactory.", "PMID": 960553}
{"id": "pubmed23n0032_3987", "title": "[Biochemical properties of different serotypes of Pasteurella haemolytica].", "content": "Studied were the morphologic and biochemic properties of 85 Pasteurella haemolytica strains belonging to the known 12 serotypes after Biberstein, Gills (2). The biochemical properties of the strains were studied after the pattern of Bosworth and Lovell (4) with regard to arabinose, trehalose, xylose, manose, salysin, and lactose. It was found that the biochemical properties correlated with the biotype classification of the strains. Thus, the biochemical reaction with trehalose and arabinose may be used as a characteristic feature in determining the biotype. The reaction proved more dependable with the strains of the T biotype as compared to those of the A biotype, as not all strains of the A biotype fermented arabinose in the course of the first seven days, while with the strains of the T biotype trehalose was acted upon during such a period. It was also established that xylose is attacked by the strains of the A biotype only, and manose and salycin--by the strains of the T biotype, which did not ferment lactose. All strains of Pasteurella haemolytica turned out to be negative so far as the production and indol was concerned; they were shown to reduce the nitrates into nitrites, were MP and VP negative, and gave growth in McConkey's agar medium.", "contents": "[Biochemical properties of different serotypes of Pasteurella haemolytica]. Studied were the morphologic and biochemic properties of 85 Pasteurella haemolytica strains belonging to the known 12 serotypes after Biberstein, Gills (2). The biochemical properties of the strains were studied after the pattern of Bosworth and Lovell (4) with regard to arabinose, trehalose, xylose, manose, salysin, and lactose. It was found that the biochemical properties correlated with the biotype classification of the strains. Thus, the biochemical reaction with trehalose and arabinose may be used as a characteristic feature in determining the biotype. The reaction proved more dependable with the strains of the T biotype as compared to those of the A biotype, as not all strains of the A biotype fermented arabinose in the course of the first seven days, while with the strains of the T biotype trehalose was acted upon during such a period. It was also established that xylose is attacked by the strains of the A biotype only, and manose and salycin--by the strains of the T biotype, which did not ferment lactose. All strains of Pasteurella haemolytica turned out to be negative so far as the production and indol was concerned; they were shown to reduce the nitrates into nitrites, were MP and VP negative, and gave growth in McConkey's agar medium.", "PMID": 960554}
{"id": "pubmed23n0032_3988", "title": "[Drug resistance of the Salmonella isolated from poultry].", "content": "A total of 92 Salmonella strains, isolated from birds, were studied through graded dilutions in a solid nutrient medium for their resistance to penicillin, streptomycin, chloramphenicol, tetracycline, furazolidon and norsulfazol. Greatest was the number of strains resistant to penicillin (81.5 per cent), and lowest was it of strains resistant to tetracycline (27.1 per cent), norsulfazol (21.7 per cent), streptomycin (15.2 per cent), and furazolidon (1.08 per cent). No resistant strains were found to chloramphenicol. Both furazolidon and chloramphenicol are suggested for the control of Salmonella infections in birds.", "contents": "[Drug resistance of the Salmonella isolated from poultry]. A total of 92 Salmonella strains, isolated from birds, were studied through graded dilutions in a solid nutrient medium for their resistance to penicillin, streptomycin, chloramphenicol, tetracycline, furazolidon and norsulfazol. Greatest was the number of strains resistant to penicillin (81.5 per cent), and lowest was it of strains resistant to tetracycline (27.1 per cent), norsulfazol (21.7 per cent), streptomycin (15.2 per cent), and furazolidon (1.08 per cent). No resistant strains were found to chloramphenicol. Both furazolidon and chloramphenicol are suggested for the control of Salmonella infections in birds.", "PMID": 960555}
{"id": "pubmed23n0032_3989", "title": "Agglutination of group Ax erythrocytes by anti-A sera (group B).", "content": "Four random examples of group Ax erythrocytes were tested with anti-A sera (group B). Microscopic examination demonstrated a high incidence of reactivity of these cells with the anti-A sera (group B) obtained after immunization with group A substance.", "contents": "Agglutination of group Ax erythrocytes by anti-A sera (group B). Four random examples of group Ax erythrocytes were tested with anti-A sera (group B). Microscopic examination demonstrated a high incidence of reactivity of these cells with the anti-A sera (group B) obtained after immunization with group A substance.", "PMID": 960678}
{"id": "pubmed23n0032_3990", "title": "Studies on new process procedures in plasma fractionation and industrial scale. III. Removal of precipitates by filtration through a horizontal leaf filter with centrifugal cleaning (Funda filter) as an alternative to tubular-bowl centrifuges.", "content": "The possibilities for replacing the time-consuming and labor-intensive centrifugation step in the plasma fractionation process with a filtration technique using filter aids were explored using a Funda filter (pat. Dr. Ing. Hans M\u00fcller). The filtration technique proved effective in the separation of the impure plasma fractions. On the other hand, its use in the separation of pure albumin and gamma-globulin fractions appears doubtful.", "contents": "Studies on new process procedures in plasma fractionation and industrial scale. III. Removal of precipitates by filtration through a horizontal leaf filter with centrifugal cleaning (Funda filter) as an alternative to tubular-bowl centrifuges. The possibilities for replacing the time-consuming and labor-intensive centrifugation step in the plasma fractionation process with a filtration technique using filter aids were explored using a Funda filter (pat. Dr. Ing. Hans M\u00fcller). The filtration technique proved effective in the separation of the impure plasma fractions. On the other hand, its use in the separation of pure albumin and gamma-globulin fractions appears doubtful.", "PMID": 960679}
{"id": "pubmed23n0032_3991", "title": "[A new method for determining the body composition with the aid of a displacement balance and low-pressure volumetric analysis (author's transl)].", "content": "A new method for determination of the specific weight of the gas-free human body is presented. Volume measurement is facilitated by extremely precise scales, by means of which a well-defined amount of water which was removed from a \"patient container\" is weighed. After the test subject has entered the container and is standing up to the neck in water, the container is closed. The combined gas volume consisting of the subject's lung volume, his intestinal gas and the air around his head is measured by lowering the pressure by an exactly-defined amount. This is done by opening a valve at the bottom of the container, which results in the outflow of a certain amount of water, the volume of which corresponds to the volume expansion inside the container. The gas volume prior to expansion can then be calculated by application of the gas laws. The advantages of this measuring device are its relatively small size, the high precision and the fact that the procedure is not unpleasant for the subject, as the head is not submerged under water.", "contents": "[A new method for determining the body composition with the aid of a displacement balance and low-pressure volumetric analysis (author's transl)]. A new method for determination of the specific weight of the gas-free human body is presented. Volume measurement is facilitated by extremely precise scales, by means of which a well-defined amount of water which was removed from a \"patient container\" is weighed. After the test subject has entered the container and is standing up to the neck in water, the container is closed. The combined gas volume consisting of the subject's lung volume, his intestinal gas and the air around his head is measured by lowering the pressure by an exactly-defined amount. This is done by opening a valve at the bottom of the container, which results in the outflow of a certain amount of water, the volume of which corresponds to the volume expansion inside the container. The gas volume prior to expansion can then be calculated by application of the gas laws. The advantages of this measuring device are its relatively small size, the high precision and the fact that the procedure is not unpleasant for the subject, as the head is not submerged under water.", "PMID": 960695}
{"id": "pubmed23n0032_3992", "title": "[Quality control of coagulation tests (author's transl)].", "content": "The results of quality control of coagulation tests from intralaboratory control programmes are reported and compared with interlaboratory surveys. The test programme includes the following assays: the thromboplastin time (Quick), the partial thromboplastin time and the thrombotest. It can be seen from the results of the thromboplastin time that reliable figures were obtained from intralaboratory quality control examinations only (CV=7% to 13%). In comparison to these results great differences were found in interlaboratory surveys (CV=15% to 30%). The result of interlaboratory surveys of the thrombotest demonstrated a CV of 15% to 20%. The intralaboratory quality control of the partial thromboplastin time showed satisfactory reliability (CV=7.4%). Satisfactory conformity of results was obtained between different laboratories only by using common reagents (CV=8.7%). Possible factors responsible for deviations in laboratory examinations are discussed and proposals for improvement in the realibility of coagulation tests are made.", "contents": "[Quality control of coagulation tests (author's transl)]. The results of quality control of coagulation tests from intralaboratory control programmes are reported and compared with interlaboratory surveys. The test programme includes the following assays: the thromboplastin time (Quick), the partial thromboplastin time and the thrombotest. It can be seen from the results of the thromboplastin time that reliable figures were obtained from intralaboratory quality control examinations only (CV=7% to 13%). In comparison to these results great differences were found in interlaboratory surveys (CV=15% to 30%). The result of interlaboratory surveys of the thrombotest demonstrated a CV of 15% to 20%. The intralaboratory quality control of the partial thromboplastin time showed satisfactory reliability (CV=7.4%). Satisfactory conformity of results was obtained between different laboratories only by using common reagents (CV=8.7%). Possible factors responsible for deviations in laboratory examinations are discussed and proposals for improvement in the realibility of coagulation tests are made.", "PMID": 960696}
{"id": "pubmed23n0032_3993", "title": "[The place of plate thermography in the diagnosis of breast cancer (author's transl)].", "content": "A report is presented of 319 unselected patients investigated by the method of plate thermography according to Tricoire, which is a comparatively new method in the diagnosis of breast cancer. 281 cases were additionally examined by mammography and those cases in whom malignancy was suspected underwent further cytological and histological investigation. The results of plate thermography and mammography are compared. The high degree of diagnostic accuracy achieved in the present series of cases by the use of plate thermography indicates that this procedure is not only a valable adjunct in the diagnosis of breast cancer but, moreover, seems to be recommendable as a screening method.", "contents": "[The place of plate thermography in the diagnosis of breast cancer (author's transl)]. A report is presented of 319 unselected patients investigated by the method of plate thermography according to Tricoire, which is a comparatively new method in the diagnosis of breast cancer. 281 cases were additionally examined by mammography and those cases in whom malignancy was suspected underwent further cytological and histological investigation. The results of plate thermography and mammography are compared. The high degree of diagnostic accuracy achieved in the present series of cases by the use of plate thermography indicates that this procedure is not only a valable adjunct in the diagnosis of breast cancer but, moreover, seems to be recommendable as a screening method.", "PMID": 960697}
{"id": "pubmed23n0032_3994", "title": "[Column chromatographic enrichment of \"glucose-6-phosphate dehydrogenase vienna\" (author's transl)].", "content": "Further biochemical investigations were performed in a case of glucose-6-phosphate dehydrogenase deficiency--called the \"Vienna\" deficiency variant on account of its biochemical characterization. It was found that the \"Vienna\" deficiency variant shows increased enzyme protein at a reduced specific activity. Gel filtration on Sephadex-G-200 produced a molecular weight identical with that of the normoenzyme; the different cochromatographic behaviour on hydroxylapatite appears attributable to a sequence modification of the amino acids.", "contents": "[Column chromatographic enrichment of \"glucose-6-phosphate dehydrogenase vienna\" (author's transl)]. Further biochemical investigations were performed in a case of glucose-6-phosphate dehydrogenase deficiency--called the \"Vienna\" deficiency variant on account of its biochemical characterization. It was found that the \"Vienna\" deficiency variant shows increased enzyme protein at a reduced specific activity. Gel filtration on Sephadex-G-200 produced a molecular weight identical with that of the normoenzyme; the different cochromatographic behaviour on hydroxylapatite appears attributable to a sequence modification of the amino acids.", "PMID": 960698}
{"id": "pubmed23n0032_3995", "title": "[Endoscopic retrograde cholangio-pancreaticography (author's transl)].", "content": "Significant advances have been achieved in the exploration of various pathological conditions of the biliary ducts by the introduction of retrograde cholangiography as diagnostic tool in this department. This investigation has proved to be very promising in view of the valuable information gained at little risk to the patient. Furthermore, retrograde pancreaticography has improved diagnostic accuracy in pancreatic diseases. However, these results should only be assessed in conjunction with the complete clinical picture and history.", "contents": "[Endoscopic retrograde cholangio-pancreaticography (author's transl)]. Significant advances have been achieved in the exploration of various pathological conditions of the biliary ducts by the introduction of retrograde cholangiography as diagnostic tool in this department. This investigation has proved to be very promising in view of the valuable information gained at little risk to the patient. Furthermore, retrograde pancreaticography has improved diagnostic accuracy in pancreatic diseases. However, these results should only be assessed in conjunction with the complete clinical picture and history.", "PMID": 960699}
{"id": "pubmed23n0032_3996", "title": "[Haemodynamic changes in acute myocardial infarction following high doses of furosemide (author's transl)].", "content": "Haemodynamic measurements were carried out after administration of furosemide to 10 patients suffering from acute myocardial infarction and congestive heart failure. It was observed that a transient deterioration in cardiac function (decreased cardiac output, increased enddiastolic pulmonary arterial pressure and increased pulmonary and systemic resistance) occured in the pre-diuretic stage in these failing hearts. After the onset of diuresis the haemodynamic parameters showed a reversal of the previous trends (increased cardiac output, decreased enddiastolic pulmonary arterial pressure and pulmonary resistance). The consistently lower enddiastolic pulmonary arterial pressure in the diuretic phase as compared with the pre-diuretic value ensured an improvement in cardiac haemodynamics. An attempt was made to interpret the haemodynamic results in the light of the Frank-Starling's curve.", "contents": "[Haemodynamic changes in acute myocardial infarction following high doses of furosemide (author's transl)]. Haemodynamic measurements were carried out after administration of furosemide to 10 patients suffering from acute myocardial infarction and congestive heart failure. It was observed that a transient deterioration in cardiac function (decreased cardiac output, increased enddiastolic pulmonary arterial pressure and increased pulmonary and systemic resistance) occured in the pre-diuretic stage in these failing hearts. After the onset of diuresis the haemodynamic parameters showed a reversal of the previous trends (increased cardiac output, decreased enddiastolic pulmonary arterial pressure and pulmonary resistance). The consistently lower enddiastolic pulmonary arterial pressure in the diuretic phase as compared with the pre-diuretic value ensured an improvement in cardiac haemodynamics. An attempt was made to interpret the haemodynamic results in the light of the Frank-Starling's curve.", "PMID": 960700}
{"id": "pubmed23n0032_3997", "title": "[A new design for a totally artificial heart: the ellipsoid heart (author's transl)].", "content": "Thromboembolic complications represent the main limiting factor in cardiac replacement by totally artificial hearts in calves at present. Thrombus formation within artificial hearts is caused by the appearance of stagnation areas. The ellipsoid heart eliminates stagnation areas by virtue of its production as a one-piece membrane. The heart is driven pneumatically and functions as a diaphragmatic blood pump. The stroke volume is 178 cm3 and the maximal cardiac output 15.8l/Min. Three acute experiments demonstrated a high degree of haemodynamic efficiency without compression of, or interference with surrounding structures, especially the inferior vena cava or the right atrium.", "contents": "[A new design for a totally artificial heart: the ellipsoid heart (author's transl)]. Thromboembolic complications represent the main limiting factor in cardiac replacement by totally artificial hearts in calves at present. Thrombus formation within artificial hearts is caused by the appearance of stagnation areas. The ellipsoid heart eliminates stagnation areas by virtue of its production as a one-piece membrane. The heart is driven pneumatically and functions as a diaphragmatic blood pump. The stroke volume is 178 cm3 and the maximal cardiac output 15.8l/Min. Three acute experiments demonstrated a high degree of haemodynamic efficiency without compression of, or interference with surrounding structures, especially the inferior vena cava or the right atrium.", "PMID": 960701}
{"id": "pubmed23n0032_3998", "title": "[The effect of \"standardized forced diuresis\" (SFD) on serum and urinary electrolytes(author's transl)].", "content": "The effect of standardized forced diuresis (SFD) on the serum and urinary electrolyte levels was investigated in 10 cases of severe self-poisoning with hypnotic drugs. Diuresis was initiated by furosemide and maintained at an hourly urinary ouput of 2 litres. Fluid and electrolyte substitution was carried out with a standardized electrolyte solution. Initiation and termination of the SFD was performed abruptly. The mean values of sodium, potassium, chloride and phosphorus in the urine varied widely at the beginning of the SFD, while the calcium and magnesium values varied only slightly. During SFD, urinary stabilization occured at a particular ionogram, in correlation to the electrolyte concentrations in the infusion fluid and with only minimal individual variation. Owing to this satisfactory correlation, none of the patients developed signs of electrolyte disturbances, so that no correction of the infusion constitution was necessary. The abrupt termination of the SFD prevented electrolyte disturbances in the recovery phase.", "contents": "[The effect of \"standardized forced diuresis\" (SFD) on serum and urinary electrolytes(author's transl)]. The effect of standardized forced diuresis (SFD) on the serum and urinary electrolyte levels was investigated in 10 cases of severe self-poisoning with hypnotic drugs. Diuresis was initiated by furosemide and maintained at an hourly urinary ouput of 2 litres. Fluid and electrolyte substitution was carried out with a standardized electrolyte solution. Initiation and termination of the SFD was performed abruptly. The mean values of sodium, potassium, chloride and phosphorus in the urine varied widely at the beginning of the SFD, while the calcium and magnesium values varied only slightly. During SFD, urinary stabilization occured at a particular ionogram, in correlation to the electrolyte concentrations in the infusion fluid and with only minimal individual variation. Owing to this satisfactory correlation, none of the patients developed signs of electrolyte disturbances, so that no correction of the infusion constitution was necessary. The abrupt termination of the SFD prevented electrolyte disturbances in the recovery phase.", "PMID": 960702}
{"id": "pubmed23n0032_3999", "title": "[The value of urinary enzyme determinations in systemic lupus erythematosus (author's transl)].", "content": "During a period of 4 years urine samples from 54 patients with systemic lupus erythematosus (SLE) were repeatedly analysed for the activities of lactate dehydrogenase (LDH), leucine aminopeptidase (LAP) and alanine aminopeptidase (AAP). Increased  urinary enzyme levels were consistently found in 8 patients with severe lupus nephritis and the nephrotic syndrome, as well as in 9 patients with chronic lupus nephritis resistant to therapy. A further group of 9 patients with lupus nephritis responded favourably to immunosuppressive therapy with arrest of kidney-damaging processes; a concomitant normalization of urinary enzyme levels was observed, giving an accurate reflection of the progression of the disease. Another 14 SLE patients showed raised enzyme levels preceding the development of clinical signs of nephropathy. The last 14 SLE patients displayed neither nephropathy nor altered enzyme activities. The determination of urinary enzyme activities is, therefore, considered to be a useful supplement to the routine biochemical analyses performed on the urine in cases of SLE.", "contents": "[The value of urinary enzyme determinations in systemic lupus erythematosus (author's transl)]. During a period of 4 years urine samples from 54 patients with systemic lupus erythematosus (SLE) were repeatedly analysed for the activities of lactate dehydrogenase (LDH), leucine aminopeptidase (LAP) and alanine aminopeptidase (AAP). Increased  urinary enzyme levels were consistently found in 8 patients with severe lupus nephritis and the nephrotic syndrome, as well as in 9 patients with chronic lupus nephritis resistant to therapy. A further group of 9 patients with lupus nephritis responded favourably to immunosuppressive therapy with arrest of kidney-damaging processes; a concomitant normalization of urinary enzyme levels was observed, giving an accurate reflection of the progression of the disease. Another 14 SLE patients showed raised enzyme levels preceding the development of clinical signs of nephropathy. The last 14 SLE patients displayed neither nephropathy nor altered enzyme activities. The determination of urinary enzyme activities is, therefore, considered to be a useful supplement to the routine biochemical analyses performed on the urine in cases of SLE.", "PMID": 960703}
{"id": "pubmed23n0032_4000", "title": "[LDH isoenzymes and gastrin in achlorhydri (author's transl)].", "content": "Several haematological findings (especially the values of serum LDH and its isoenzymes) were compared with changes in the gastrin level in pernicious anaemia. While vitamin B12 substitution therapy led to normalization of the anaemia and of the enzyme levels, gastric atrophy and, hence, the elevation in serum gastrin levels remained unchanged. Determination of serum gastrin, therefore, provides a valuable tool for the verification of the diagnosis of pernicious anaemia in treated cases.", "contents": "[LDH isoenzymes and gastrin in achlorhydri (author's transl)]. Several haematological findings (especially the values of serum LDH and its isoenzymes) were compared with changes in the gastrin level in pernicious anaemia. While vitamin B12 substitution therapy led to normalization of the anaemia and of the enzyme levels, gastric atrophy and, hence, the elevation in serum gastrin levels remained unchanged. Determination of serum gastrin, therefore, provides a valuable tool for the verification of the diagnosis of pernicious anaemia in treated cases.", "PMID": 960704}
{"id": "pubmed23n0032_4001", "title": "[Considerations on the information content of diagnostic tests (author's transl)].", "content": "The value of a diagnostic test lies in its ability to support the doctor's efforts to detect a certain disease. The aim is to achieve the highest possible probability that the disease is correctly diagnosed by means of the test. Doctors are not always aware of the extent to which a test can contribute to the final diagnosis. This contribution, the information content of the test, can be calculated by various algebraic manipulations. An example (carcinoembryonic antigen) is given, which demonstrates the significance of certain terms such as true and false positive results or likelihood and, moreover, the application of Baye's theorem.", "contents": "[Considerations on the information content of diagnostic tests (author's transl)]. The value of a diagnostic test lies in its ability to support the doctor's efforts to detect a certain disease. The aim is to achieve the highest possible probability that the disease is correctly diagnosed by means of the test. Doctors are not always aware of the extent to which a test can contribute to the final diagnosis. This contribution, the information content of the test, can be calculated by various algebraic manipulations. An example (carcinoembryonic antigen) is given, which demonstrates the significance of certain terms such as true and false positive results or likelihood and, moreover, the application of Baye's theorem.", "PMID": 960705}
{"id": "pubmed23n0032_4002", "title": "[Clinical aspects of cystinuria and its treatment by thiola (author's transl)].", "content": "Cystinuria is an inherited disorder of amino acid transport affecting the epithelial cells of the renal tubules and the gastro-intestinal tract. Treatment consists of the prophylaxis of recurrent urolithiasis, which is the clinical manifestation of the disease. Long-term treatment with alpha-mercapto-propionyl-glycine (MPG; Thiola) promises to be successful. 3 cytinuric patients with recurrent urolithiasis underwent treatment over a period of 6 months. Therapy was controlled by regular follow-up investigations of the urinary excretion and serum levels of cystine and di-basic amino acids. The results did not indicate any permanent decrease in cystine excretion. No recurrence of renal calculi was observed. The possibility is discussed of a direkt mechanism of action of the drug on the metabolism of the involved amino acids.", "contents": "[Clinical aspects of cystinuria and its treatment by thiola (author's transl)]. Cystinuria is an inherited disorder of amino acid transport affecting the epithelial cells of the renal tubules and the gastro-intestinal tract. Treatment consists of the prophylaxis of recurrent urolithiasis, which is the clinical manifestation of the disease. Long-term treatment with alpha-mercapto-propionyl-glycine (MPG; Thiola) promises to be successful. 3 cytinuric patients with recurrent urolithiasis underwent treatment over a period of 6 months. Therapy was controlled by regular follow-up investigations of the urinary excretion and serum levels of cystine and di-basic amino acids. The results did not indicate any permanent decrease in cystine excretion. No recurrence of renal calculi was observed. The possibility is discussed of a direkt mechanism of action of the drug on the metabolism of the involved amino acids.", "PMID": 960706}
{"id": "pubmed23n0032_4003", "title": "[Extra-articular manifestations of chronic polyarthritis].", "content": "Although the chronic destructive joint processes dominate the clinical picture of chronic rheumatoid arthritis, it must not been forgotten that the disease is a systemic one, whereby certain organs or organ systems are affected either clinically or subclinically. The various possible pathogenetic mechanisms are discussed and the particular type of patient with rheumatoid arthritis who appears to be prone to extra-articular manifestations is described. Subsequently, the individual organic manifestations and the clinical picture are discussed, as well as the incidence and significance of these features.", "contents": "[Extra-articular manifestations of chronic polyarthritis]. Although the chronic destructive joint processes dominate the clinical picture of chronic rheumatoid arthritis, it must not been forgotten that the disease is a systemic one, whereby certain organs or organ systems are affected either clinically or subclinically. The various possible pathogenetic mechanisms are discussed and the particular type of patient with rheumatoid arthritis who appears to be prone to extra-articular manifestations is described. Subsequently, the individual organic manifestations and the clinical picture are discussed, as well as the incidence and significance of these features.", "PMID": 960707}
{"id": "pubmed23n0032_4004", "title": "[The implementation of a computerized documentation system for the evaluation of spa therapy in patients with rheumatoid arthritis (author's transl)].", "content": "A previously-described documentation system for articular examination was applied to evaluate the results of spa treatment in 20 patients with rheumatoid arthritis during 3 separate treatment periods. Physical joint findings were documented and indices were compiled by means of a computer before, during and after treatment with thermal water the first year, normal water the second year (or vice versa) and without baths at all in the third year. These indices were compared statistically. Each kind of treatment produced a statistically-significant improvement in the disease, there being no significant difference in the results achieved by the 3 therapeutic regimens.", "contents": "[The implementation of a computerized documentation system for the evaluation of spa therapy in patients with rheumatoid arthritis (author's transl)]. A previously-described documentation system for articular examination was applied to evaluate the results of spa treatment in 20 patients with rheumatoid arthritis during 3 separate treatment periods. Physical joint findings were documented and indices were compiled by means of a computer before, during and after treatment with thermal water the first year, normal water the second year (or vice versa) and without baths at all in the third year. These indices were compared statistically. Each kind of treatment produced a statistically-significant improvement in the disease, there being no significant difference in the results achieved by the 3 therapeutic regimens.", "PMID": 960708}
{"id": "pubmed23n0032_4005", "title": "[Cytogenetic investigations in patients receiving an intraarticular injection of gold-198 (author's transl)].", "content": "The chromosomes of lymphocyte cultures from the peripheral blood were investigated in 10 patients (rheumatoid arthritis, ankylosing spondylitis, osteoarthrosis of the knee) before and at short-term intervals (1 day, 3 to 4 days, 14 to 18 days) after the injection of gold-198 (5 to 20 mC; usually 8 mC; average particle size 300 A) into the knee-joint. The number of structural chromosomal abberrations increased markedly in 4 of the 10 patients. On the first day after the gold injection the mean aberration rate was significantly higher than the control value (Chi2-5.18; df=1; P less than 0.05). Most of the observed aberrations were chromatid aberrations and this finding is discussed.", "contents": "[Cytogenetic investigations in patients receiving an intraarticular injection of gold-198 (author's transl)]. The chromosomes of lymphocyte cultures from the peripheral blood were investigated in 10 patients (rheumatoid arthritis, ankylosing spondylitis, osteoarthrosis of the knee) before and at short-term intervals (1 day, 3 to 4 days, 14 to 18 days) after the injection of gold-198 (5 to 20 mC; usually 8 mC; average particle size 300 A) into the knee-joint. The number of structural chromosomal abberrations increased markedly in 4 of the 10 patients. On the first day after the gold injection the mean aberration rate was significantly higher than the control value (Chi2-5.18; df=1; P less than 0.05). Most of the observed aberrations were chromatid aberrations and this finding is discussed.", "PMID": 960709}
{"id": "pubmed23n0032_4006", "title": "[Congenital polyfibromatosis: clinical and genetic studies].", "content": "Clinical and genetic investigations were performed on seven members of a family suffering from multiple hereditary fibromatosis. These studies indicate that the responsible gene appears to be coupled and transmitted with that of the rhesus system, located at chromosome one. The diagnosis, differential diagnosis and therapy, as well as histological alterations are pointed out and the importance of hormonal factors is discussed.", "contents": "[Congenital polyfibromatosis: clinical and genetic studies]. Clinical and genetic investigations were performed on seven members of a family suffering from multiple hereditary fibromatosis. These studies indicate that the responsible gene appears to be coupled and transmitted with that of the rhesus system, located at chromosome one. The diagnosis, differential diagnosis and therapy, as well as histological alterations are pointed out and the importance of hormonal factors is discussed.", "PMID": 960710}
{"id": "pubmed23n0032_4007", "title": "[Subclavian vein catheterization in traumatic surgery (author's transl)A1].", "content": "The new Cavafix system was employed for puncture and catheterization of the subclavian vein in 100 casuality patients undergoing surgical intervention. Excellent results were achieved not only in emergency cases with severe shock and hypovolaemia, but also in hospitalized patients requiring long-term infusion therapy. This system proved easy to handle even by doctors inexperienced in the use of such techniques and, hence, the incidence of complications was within the range reported in the literature.", "contents": "[Subclavian vein catheterization in traumatic surgery (author's transl)A1]. The new Cavafix system was employed for puncture and catheterization of the subclavian vein in 100 casuality patients undergoing surgical intervention. Excellent results were achieved not only in emergency cases with severe shock and hypovolaemia, but also in hospitalized patients requiring long-term infusion therapy. This system proved easy to handle even by doctors inexperienced in the use of such techniques and, hence, the incidence of complications was within the range reported in the literature.", "PMID": 960711}
{"id": "pubmed23n0032_4008", "title": "[The treatment of osseous rupture of the posterior cruciate ligament (author's transl)].", "content": "3 cases are reported of successful surgical treatment of isolated osseous rupture of the posterior cruciate ligament. Fixation was undertaken with 2 crossed wires leading from the popliteal fossa to the anterior aspect of the tibia. The advantage of this method is that the popliteal fossa does not have to be reopened 6 to 8 weeks later when the wires are removed.", "contents": "[The treatment of osseous rupture of the posterior cruciate ligament (author's transl)]. 3 cases are reported of successful surgical treatment of isolated osseous rupture of the posterior cruciate ligament. Fixation was undertaken with 2 crossed wires leading from the popliteal fossa to the anterior aspect of the tibia. The advantage of this method is that the popliteal fossa does not have to be reopened 6 to 8 weeks later when the wires are removed.", "PMID": 960712}
{"id": "pubmed23n0032_4009", "title": "[Percutaneous reduction of fractures of the neck of the radium in children by means of a steinmann nail (author's transl)].", "content": "Percutaneous reduction of fractures of the neck of the radius or of epiphysiolysis with displacement can be achieved in children by means of a Steinmann nail. No additional means of fragment fixation seems to be necessary. Fracture healing is ensured by cast immobilization. Functional results are equivalent to those after conservative treatment.", "contents": "[Percutaneous reduction of fractures of the neck of the radium in children by means of a steinmann nail (author's transl)]. Percutaneous reduction of fractures of the neck of the radius or of epiphysiolysis with displacement can be achieved in children by means of a Steinmann nail. No additional means of fragment fixation seems to be necessary. Fracture healing is ensured by cast immobilization. Functional results are equivalent to those after conservative treatment.", "PMID": 960713}
{"id": "pubmed23n0032_4010", "title": "[Iatrogenic tissue damage and its treatment (author's transl)].", "content": "Modern medicine with its manifold therapeutic possibilites brings many side effects which might result in tissue damage. 8 cases of iatrogenic tissue damage seen over a period of 5 years are presented and discussed. Measures for correction and reconstruction are proposed.", "contents": "[Iatrogenic tissue damage and its treatment (author's transl)]. Modern medicine with its manifold therapeutic possibilites brings many side effects which might result in tissue damage. 8 cases of iatrogenic tissue damage seen over a period of 5 years are presented and discussed. Measures for correction and reconstruction are proposed.", "PMID": 960714}
{"id": "pubmed23n0032_4011", "title": "[Scalp avulsion injuries (author's transl)].", "content": "In a recent scalp avulsion injury, the scalp should always be re-implanted without delay if at all available. Subsequent normal hair growth can hardly be expected, but at least an original skin replacement has been effected. The reconstruction of only a part of the scalp should be performed in such a way that the hairless area is situated in the centre. The surrounding hair will cover this centre part. Rotation flaps in the region of the scalp follow other rules than apply to all other areas of the body. If applied correctly, good results can be achieved because of the excellent blood supply.", "contents": "[Scalp avulsion injuries (author's transl)]. In a recent scalp avulsion injury, the scalp should always be re-implanted without delay if at all available. Subsequent normal hair growth can hardly be expected, but at least an original skin replacement has been effected. The reconstruction of only a part of the scalp should be performed in such a way that the hairless area is situated in the centre. The surrounding hair will cover this centre part. Rotation flaps in the region of the scalp follow other rules than apply to all other areas of the body. If applied correctly, good results can be achieved because of the excellent blood supply.", "PMID": 960715}
{"id": "pubmed23n0032_4012", "title": "Preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus DNA synthesis and virus-induced DNA polymerase activity.", "content": "In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 \u03bcg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent.", "contents": "Preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus DNA synthesis and virus-induced DNA polymerase activity. In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 \u03bcg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent.", "PMID": 960726}
{"id": "pubmed23n0032_4013", "title": "Viral hepatitis in the Air Force.", "content": "The reported incidence of viral hepatitis in the Air Force has increased over the past 10 years. The total number of days lost from duty has declined as has the average number of days lost per case. Distribution of USAF reported cases has been roughly equally divided among the three diagnostic categories, in contrast to the total United States reported distribution. Relatively few USAF cases have had a documented history of drug abuse since we began collecting this information, and the proportion of these cases has steadily declined. Finally, these diseases still represent significant economic and operational costs to the Air Force so that prevention and control remain important items of concern to commanders and the medical service.", "contents": "Viral hepatitis in the Air Force. The reported incidence of viral hepatitis in the Air Force has increased over the past 10 years. The total number of days lost from duty has declined as has the average number of days lost per case. Distribution of USAF reported cases has been roughly equally divided among the three diagnostic categories, in contrast to the total United States reported distribution. Relatively few USAF cases have had a documented history of drug abuse since we began collecting this information, and the proportion of these cases has steadily declined. Finally, these diseases still represent significant economic and operational costs to the Air Force so that prevention and control remain important items of concern to commanders and the medical service.", "PMID": 960727}
{"id": "pubmed23n0032_4014", "title": "Viral hepatitis in the United States Navy and Marine Corps.", "content": "Two analyses of the Navy and Marine Corps experience with viral hepatitis are reported. The first is longitudinal in nature and shows that over the past 100 years the syndrome has been common and at a relatively steady rate of 100-400 cases/100,000 average strength/year. The second is an examination of cases in calendar year 1974 for demographic, geographic, and occupational patterns. There is a strong inverse relationship between disease occurrence and age. Each type of viral hepatitis shows a distinct geographic distribution. Navy personnel in health-related occupations had a greater risk of acquiring viral hepatitis than did other persons on active duty. Differences among other occupational groups were found, but valid interpretations could not be made because of the small numbers of cases in each category.", "contents": "Viral hepatitis in the United States Navy and Marine Corps. Two analyses of the Navy and Marine Corps experience with viral hepatitis are reported. The first is longitudinal in nature and shows that over the past 100 years the syndrome has been common and at a relatively steady rate of 100-400 cases/100,000 average strength/year. The second is an examination of cases in calendar year 1974 for demographic, geographic, and occupational patterns. There is a strong inverse relationship between disease occurrence and age. Each type of viral hepatitis shows a distinct geographic distribution. Navy personnel in health-related occupations had a greater risk of acquiring viral hepatitis than did other persons on active duty. Differences among other occupational groups were found, but valid interpretations could not be made because of the small numbers of cases in each category.", "PMID": 960729}
{"id": "pubmed23n0032_4015", "title": "Viral hepatitis: problems of incidence and control in military personnel.", "content": "(A) Hepatitis B virus (HBV) is now the major cause of infectious viral hepatitis in U.S. military personnel and probably also in the civilian population over 15 years of age. (B) The incidence of icteric, viral hepatitis is much higher in U.S. military personnel than in comparable age groups in the civilian population. The 17-to 20-year-old enlisted men show the highest rates. (C) In parts of the world (e.g., U.S.A., Germany) where most of the inapparent infection is caused by the adw subtype of HBV, most of the acute clinical disease is caused by the ayw subtype. In the U.S.A. and Germany, 95% or more of HB(s) Ag isolates from U.S. military personnel with acute hepatitis is ayw. (D) It may be many years before one can expect to have sufficient data for a decision as to the possible availability of an effective HBV vaccine. Accordingly, a decision is urgently needed regarding either the immediate use of the best practically available hepatitis immune gamma globulin, that can be prepared by modern techniques, for the prevention of hepatitis in U.S. military personnel or postponement of such use until an adequate and properly controlled trial can be carried out in active duty military personnel in an area of high incidence.", "contents": "Viral hepatitis: problems of incidence and control in military personnel. (A) Hepatitis B virus (HBV) is now the major cause of infectious viral hepatitis in U.S. military personnel and probably also in the civilian population over 15 years of age. (B) The incidence of icteric, viral hepatitis is much higher in U.S. military personnel than in comparable age groups in the civilian population. The 17-to 20-year-old enlisted men show the highest rates. (C) In parts of the world (e.g., U.S.A., Germany) where most of the inapparent infection is caused by the adw subtype of HBV, most of the acute clinical disease is caused by the ayw subtype. In the U.S.A. and Germany, 95% or more of HB(s) Ag isolates from U.S. military personnel with acute hepatitis is ayw. (D) It may be many years before one can expect to have sufficient data for a decision as to the possible availability of an effective HBV vaccine. Accordingly, a decision is urgently needed regarding either the immediate use of the best practically available hepatitis immune gamma globulin, that can be prepared by modern techniques, for the prevention of hepatitis in U.S. military personnel or postponement of such use until an adequate and properly controlled trial can be carried out in active duty military personnel in an area of high incidence.", "PMID": 960730}
{"id": "pubmed23n0032_4016", "title": "Yale studies of patient care. I. The evaluation of the febrile patient.", "content": "Observations are reported on the variation in evaluation and management of 216 episodes of fever in 690 patients on four services of a university hospital. Twenty-two percent of febrile episodes were not commented upon in the medical record. Thirty percent of all fevers and 14% of antibiotic-treated fevers were not evaluated with microbiologic cultures. The extent of evaluation varied with service and varied directly with the height of the fever and the clinical recording of abnormality in temperature.", "contents": "Yale studies of patient care. I. The evaluation of the febrile patient. Observations are reported on the variation in evaluation and management of 216 episodes of fever in 690 patients on four services of a university hospital. Twenty-two percent of febrile episodes were not commented upon in the medical record. Thirty percent of all fevers and 14% of antibiotic-treated fevers were not evaluated with microbiologic cultures. The extent of evaluation varied with service and varied directly with the height of the fever and the clinical recording of abnormality in temperature.", "PMID": 960731}
{"id": "pubmed23n0032_4017", "title": "[Protein crystals and tubuli bundles in yeast cells. IV. Biochemical and electron microscopic studies of the induction of alcohol dehydrogenase (ADH) crystals].", "content": "Cells of Saccharomyces carlsbergensis H 60 synthesize alcohol dehydrogenase (ADH) in media containing 2% lactat, 1% ethanol or 0.1% glucose. Crystals may be induced in protoplasts of these cells. Increase of glucose concentration in the medium results in diminished ADH synthesis and decreased tendency for crystal formation. Repression of ADH synthesis by glucose results in the formation of a protein (MG 110000 D), the significance of which is discussed. Early stages of crystal formation inside the cell are demonstrated electronmicroscopically. At first dense material accumulates between opposite membranes of neighbouring mitochondria. Within mitochondria frequently membrane bundles occur in close vicinity to crystals. These ADH-crystals arise from this material.", "contents": "[Protein crystals and tubuli bundles in yeast cells. IV. Biochemical and electron microscopic studies of the induction of alcohol dehydrogenase (ADH) crystals]. Cells of Saccharomyces carlsbergensis H 60 synthesize alcohol dehydrogenase (ADH) in media containing 2% lactat, 1% ethanol or 0.1% glucose. Crystals may be induced in protoplasts of these cells. Increase of glucose concentration in the medium results in diminished ADH synthesis and decreased tendency for crystal formation. Repression of ADH synthesis by glucose results in the formation of a protein (MG 110000 D), the significance of which is discussed. Early stages of crystal formation inside the cell are demonstrated electronmicroscopically. At first dense material accumulates between opposite membranes of neighbouring mitochondria. Within mitochondria frequently membrane bundles occur in close vicinity to crystals. These ADH-crystals arise from this material.", "PMID": 960735}
{"id": "pubmed23n0032_4018", "title": "[Disturbances of consciousness of old people from internal view (author's transl)].", "content": "Any unconsciousness and especially that of old people refers to direct danger to life and requires immediate medical intervening. A diagnostic plan will not be adequate in association to the multifariour etiological factors and can delay in a special case the beginning of an aimed therapy. The unconscious patient must have a clinical treatment. The author describes the diagnostic of the most important comatose states in the age.", "contents": "[Disturbances of consciousness of old people from internal view (author's transl)]. Any unconsciousness and especially that of old people refers to direct danger to life and requires immediate medical intervening. A diagnostic plan will not be adequate in association to the multifariour etiological factors and can delay in a special case the beginning of an aimed therapy. The unconscious patient must have a clinical treatment. The author describes the diagnostic of the most important comatose states in the age.", "PMID": 960736}
{"id": "pubmed23n0032_4019", "title": "[Changes depend on age in electromyogram -- studies about parameter of motor unit action potentials in normal subjects of different age (author's transl)].", "content": "In normal subjects of different age groups electromyographically several muscles were examined. The motor unit action potentials were registered and parameters of those were evaluated manually and digitally. For all examined muscles was found an increase in the duration of potentials with the age. The increase was greatest in the younger and older age groups. An interpretation of these findings is given.", "contents": "[Changes depend on age in electromyogram -- studies about parameter of motor unit action potentials in normal subjects of different age (author's transl)]. In normal subjects of different age groups electromyographically several muscles were examined. The motor unit action potentials were registered and parameters of those were evaluated manually and digitally. For all examined muscles was found an increase in the duration of potentials with the age. The increase was greatest in the younger and older age groups. An interpretation of these findings is given.", "PMID": 960737}
{"id": "pubmed23n0032_4020", "title": "[Ageing an expression of interrelation between organs and tissue of organism (author's transl)].", "content": "The authors starts from results of investigations carried out by himself, dealing with biological cell criteria of organ cultures after the donor animals had been X-rayed on their entire bodies. He discusses the problem whether ageing is an inherent character in all cells. After juvenile rats had been X-rayed, organ cultures of their milts and kidneys showed alterations analogous to those of \"old\" donor animals. These alterations could not be detected if the animals were killed immediately after X-raying and the cultures were taken then. The alteration of biological cell criteria are discussed as an expression of interrelations between organs and tissues of organism.", "contents": "[Ageing an expression of interrelation between organs and tissue of organism (author's transl)]. The authors starts from results of investigations carried out by himself, dealing with biological cell criteria of organ cultures after the donor animals had been X-rayed on their entire bodies. He discusses the problem whether ageing is an inherent character in all cells. After juvenile rats had been X-rayed, organ cultures of their milts and kidneys showed alterations analogous to those of \"old\" donor animals. These alterations could not be detected if the animals were killed immediately after X-raying and the cultures were taken then. The alteration of biological cell criteria are discussed as an expression of interrelations between organs and tissues of organism.", "PMID": 960739}
{"id": "pubmed23n0032_4021", "title": "[On the metabolic heterogenity of the cartilage tissue (author's transl)].", "content": "Autoradiografic studies with 3H-thymidine, 3H-proline and 35S-sulfate in different cartilage tissues from rabbits and rats (articular and epiphyseal cartilage of the knee; costal, xiphoid and tracheal cartilage) were performed. The following results were obtained: The proliferative zone of the epiphyseal plate of young and adult animals is limited to the superior part of the columnar cartilage. The articular cartilage shows no proliferative zones but single S-phase-nuclei in all layers seldom were found. In the adult animals a decrease of DNA-synthetising chondrocytes in all investigated cartilage tissues and a narrowing of the proliferative zone could be detected. 3H-proline and 35S-sulfate in proliferating chondrocytes of the bone cartilage border line and in chondrocytes of the subperichondrial area increasingly were incorporated. Possible causes of the regional differences in the uptake of the used precursors were discussed and the significance of the metabolic heterogenity of the cartilage tissue for aging and pathological processes was pointed to.", "contents": "[On the metabolic heterogenity of the cartilage tissue (author's transl)]. Autoradiografic studies with 3H-thymidine, 3H-proline and 35S-sulfate in different cartilage tissues from rabbits and rats (articular and epiphyseal cartilage of the knee; costal, xiphoid and tracheal cartilage) were performed. The following results were obtained: The proliferative zone of the epiphyseal plate of young and adult animals is limited to the superior part of the columnar cartilage. The articular cartilage shows no proliferative zones but single S-phase-nuclei in all layers seldom were found. In the adult animals a decrease of DNA-synthetising chondrocytes in all investigated cartilage tissues and a narrowing of the proliferative zone could be detected. 3H-proline and 35S-sulfate in proliferating chondrocytes of the bone cartilage border line and in chondrocytes of the subperichondrial area increasingly were incorporated. Possible causes of the regional differences in the uptake of the used precursors were discussed and the significance of the metabolic heterogenity of the cartilage tissue for aging and pathological processes was pointed to.", "PMID": 960740}
{"id": "pubmed23n0032_4022", "title": "[Age dependent changes of biosynthesis of proteins (author's transl)].", "content": "The activity of KC1-washed ribosomes from the liver and the kidney of rats in a poly(U) dependent cell free system with [3H]-phe-tRNA as phenylalanine donor is highly increased by the cytosolic fraction from liver, brain, kidney and testicles. The activity of the cell sap of these tissues decreases significantly with increasing age of the rats from which the cytosolic fractions were prepared. Ribosomes do not undergo age dependent alterations with regard to their activity in poly(U) systems supported by cell sap from rat liver. The results are discussed with regard to age dependent change of the content of cytosolic translation factors.", "contents": "[Age dependent changes of biosynthesis of proteins (author's transl)]. The activity of KC1-washed ribosomes from the liver and the kidney of rats in a poly(U) dependent cell free system with [3H]-phe-tRNA as phenylalanine donor is highly increased by the cytosolic fraction from liver, brain, kidney and testicles. The activity of the cell sap of these tissues decreases significantly with increasing age of the rats from which the cytosolic fractions were prepared. Ribosomes do not undergo age dependent alterations with regard to their activity in poly(U) systems supported by cell sap from rat liver. The results are discussed with regard to age dependent change of the content of cytosolic translation factors.", "PMID": 960741}
{"id": "pubmed23n0032_4023", "title": "[Ageing-changes in collagen -- biosynthesis of collagen (part II) (author's transl)].", "content": "In the present three parts of the survey on ageing-changes in collagen physical and chemical qualities are discussed at first. Further a summary on questions of the biosynthesis of collagen, changes of the collagen metabolism in the ageing organism organism and changes of the ripening collagen in the living organism is given. Following in vitro-ageing-changes of collagen and causes of the changes of the ageing organism are described. A summarizing appreciation of the ageing-changes in the collagen finishes the survey.", "contents": "[Ageing-changes in collagen -- biosynthesis of collagen (part II) (author's transl)]. In the present three parts of the survey on ageing-changes in collagen physical and chemical qualities are discussed at first. Further a summary on questions of the biosynthesis of collagen, changes of the collagen metabolism in the ageing organism organism and changes of the ripening collagen in the living organism is given. Following in vitro-ageing-changes of collagen and causes of the changes of the ageing organism are described. A summarizing appreciation of the ageing-changes in the collagen finishes the survey.", "PMID": 960742}
{"id": "pubmed23n0032_4024", "title": "[Development and organisation of cardiorespiratory function testing in GDR (author's transl)].", "content": "Acute and chronic respiratory diseases amount to a remarkable portion of all cases and days of disability. The systematic care especially for the chronic respiratory diseases has become the task of the Out Patient Departments of Pulmonary Diseases and Tuberculosis in cooperation with the general practitioners and physicians for industrial medicine. The diagnostics of chronic respiratory diseases among which more than the half of all cases gets to chronic bronchitis, pulmonary emphysema and bronchial asthma has still to be qualified. Function testing allows to subdivide chronic bronchitis in nonobstructive and obstructive forms and to gain measurable and comparable assessments of cardiopulmonary limitations of efficiency. By oxyergotensiometry and examination of the diffusion capacity disturbances of ventilation, perfusion and diffusion and combinations of these can be analyzed by blood-gas analysis, ergometric examination and testing of diffusion capacity. Those investigations can be supplemented by determination of breathing mechanics. The development of cardiorespiratory function testing will take place step by step in different levels of accomplishment. These levels have to cooperate for getting an optimal picture of a disease. The extension of the methods of pulmonary function testing in the lung hospitals and the chest clinics since 1970 is reported.", "contents": "[Development and organisation of cardiorespiratory function testing in GDR (author's transl)]. Acute and chronic respiratory diseases amount to a remarkable portion of all cases and days of disability. The systematic care especially for the chronic respiratory diseases has become the task of the Out Patient Departments of Pulmonary Diseases and Tuberculosis in cooperation with the general practitioners and physicians for industrial medicine. The diagnostics of chronic respiratory diseases among which more than the half of all cases gets to chronic bronchitis, pulmonary emphysema and bronchial asthma has still to be qualified. Function testing allows to subdivide chronic bronchitis in nonobstructive and obstructive forms and to gain measurable and comparable assessments of cardiopulmonary limitations of efficiency. By oxyergotensiometry and examination of the diffusion capacity disturbances of ventilation, perfusion and diffusion and combinations of these can be analyzed by blood-gas analysis, ergometric examination and testing of diffusion capacity. Those investigations can be supplemented by determination of breathing mechanics. The development of cardiorespiratory function testing will take place step by step in different levels of accomplishment. These levels have to cooperate for getting an optimal picture of a disease. The extension of the methods of pulmonary function testing in the lung hospitals and the chest clinics since 1970 is reported.", "PMID": 960763}
{"id": "pubmed23n0032_4025", "title": "Models of respiratory insufficiency in view of lung function diagnostics and pathogenetic analysis (author's transl).", "content": "Organizing principles of lung function diagnostics and of research in the field of pathophysiology of respiratory insufficiency are developed starting from fundamental requirements, which are practical effectivity, scientific basement, technical development and teamwork of specialists in a complementary system. Technique of screening level, primary oriented of effectivity, is based on the simple monoalveolar model of ventilation. Basal lung function diagnostics, primary oriented on objectivity, representing the total of pulmonary impairment is based on the model  of arterial hypoxemia. Condition and limitations of these models are discussed preparing its substitution by models of complex lung function analysis in the higher level diagnostic system.", "contents": "Models of respiratory insufficiency in view of lung function diagnostics and pathogenetic analysis (author's transl). Organizing principles of lung function diagnostics and of research in the field of pathophysiology of respiratory insufficiency are developed starting from fundamental requirements, which are practical effectivity, scientific basement, technical development and teamwork of specialists in a complementary system. Technique of screening level, primary oriented of effectivity, is based on the simple monoalveolar model of ventilation. Basal lung function diagnostics, primary oriented on objectivity, representing the total of pulmonary impairment is based on the model  of arterial hypoxemia. Condition and limitations of these models are discussed preparing its substitution by models of complex lung function analysis in the higher level diagnostic system.", "PMID": 960764}
{"id": "pubmed23n0032_4026", "title": "[Pathophysiology of respiratory disturbances in children (author's transl)].", "content": "Physiological conditions of respiration in children are defined especially by - relative hyperventilation because of high oxygen uptake per body surface, - relative narrow and soft airways with high tendency to obstruction, causing atelectasis, pneumonia or severe bronchiolitis. It is useful to differentiate between bronchiolitis and spastic or asthmatoid bronchitis, the latter being sensible to Adrenalin and developing to asthma of adults. Characteristical signs of asthmatoid bronchitis are bronchial hyperreactivity, increased airway-resistance and residual volume, decreased FEV 1, pulmonary compliance, arterial PO2 and PCO2 with signs of pulmonary inhomogeneity. Mucviscidosis, starting from abnormal viscosity of bronchial secretion, is functionally characterized by similar signs, so are increased RV with air-trapping, decreased FEV 1, VC, PO2a and pulmonary inhomogeneity. Diffuse progressive interstitial pulmonary fibrosis (HAMMAN-RICH) of acute type being mostly lethal in children up to 2 years of age and of subacute type in older children shows diffusion disturbance and characteristical ventilation disturbance with reduction of inspiratory reserve volume and enlargement of functional residual capacity but normal FEV 1. Disturbances are sensible to corticoid-therapy.", "contents": "[Pathophysiology of respiratory disturbances in children (author's transl)]. Physiological conditions of respiration in children are defined especially by - relative hyperventilation because of high oxygen uptake per body surface, - relative narrow and soft airways with high tendency to obstruction, causing atelectasis, pneumonia or severe bronchiolitis. It is useful to differentiate between bronchiolitis and spastic or asthmatoid bronchitis, the latter being sensible to Adrenalin and developing to asthma of adults. Characteristical signs of asthmatoid bronchitis are bronchial hyperreactivity, increased airway-resistance and residual volume, decreased FEV 1, pulmonary compliance, arterial PO2 and PCO2 with signs of pulmonary inhomogeneity. Mucviscidosis, starting from abnormal viscosity of bronchial secretion, is functionally characterized by similar signs, so are increased RV with air-trapping, decreased FEV 1, VC, PO2a and pulmonary inhomogeneity. Diffuse progressive interstitial pulmonary fibrosis (HAMMAN-RICH) of acute type being mostly lethal in children up to 2 years of age and of subacute type in older children shows diffusion disturbance and characteristical ventilation disturbance with reduction of inspiratory reserve volume and enlargement of functional residual capacity but normal FEV 1. Disturbances are sensible to corticoid-therapy.", "PMID": 960765}
{"id": "pubmed23n0032_4027", "title": "[New aspects in the pathophysiology of bronchial asthma (author's transl)].", "content": "Bronchial asthma is a polyetiological syndrome. Not only different immunological reactions (atopic reactions, immune-complex reaction, cell mediated sensitisation) but also unspecific mechanisms are of importance. Bronchial obstruction becomes manifest with bronchial spasm, edema, and dyscrinia especially in the small airways. Methods are described for diagnosis of small airways obstruction. Inhomogenous bronchial obstruction is of paramount importance for the disturbances of oxygenation.", "contents": "[New aspects in the pathophysiology of bronchial asthma (author's transl)]. Bronchial asthma is a polyetiological syndrome. Not only different immunological reactions (atopic reactions, immune-complex reaction, cell mediated sensitisation) but also unspecific mechanisms are of importance. Bronchial obstruction becomes manifest with bronchial spasm, edema, and dyscrinia especially in the small airways. Methods are described for diagnosis of small airways obstruction. Inhomogenous bronchial obstruction is of paramount importance for the disturbances of oxygenation.", "PMID": 960766}
{"id": "pubmed23n0032_4028", "title": "[Experimental model of bronchial asthma (author's transl)].", "content": "Allergic asthma-like attacks were induced in guinea pigs by intraperitoneal injections of Ovalbumin and following inhalation of Ovalbumin aerosol. The animals were narcotized, tracheotomized and a small catheter was placed in mid-oesophagus. The quantities: oesophageal pressure, respiratory flow and tidal volume were measured and recorded on magnetic tape during spontaneous breathing. A technique was applied for automated computation of the parameters of respiratory mechanics using a digital computer. The parameters compliance, resistance, work of breathing and power of breathing showed typical pathological variances and characteristic response-curves in the experimental phases during and after the asthma attacks. These parameters became normal after the injection of Alupent.", "contents": "[Experimental model of bronchial asthma (author's transl)]. Allergic asthma-like attacks were induced in guinea pigs by intraperitoneal injections of Ovalbumin and following inhalation of Ovalbumin aerosol. The animals were narcotized, tracheotomized and a small catheter was placed in mid-oesophagus. The quantities: oesophageal pressure, respiratory flow and tidal volume were measured and recorded on magnetic tape during spontaneous breathing. A technique was applied for automated computation of the parameters of respiratory mechanics using a digital computer. The parameters compliance, resistance, work of breathing and power of breathing showed typical pathological variances and characteristic response-curves in the experimental phases during and after the asthma attacks. These parameters became normal after the injection of Alupent.", "PMID": 960767}
{"id": "pubmed23n0032_4029", "title": "[Tonus of respiratory muscles in acetycholine hypotonia (author's transl)].", "content": "The importance of respiratory muscles in relation to circulation and ventilation was investigated in acute hypotonia after acetylcholin injection. The tonus of respiratory muscles was assessed by measuring the circumference of chest and abdomen. Changes of volume of the body cavities were examined by body plethysmography. A significant relation between the increase of circumferences of the chest and abdomen and increase of lung volume was registered. The authors conclude, that the tonus of the respiratory muscles determines the volume of chest and abdomen and influences the distribution of blood.", "contents": "[Tonus of respiratory muscles in acetycholine hypotonia (author's transl)]. The importance of respiratory muscles in relation to circulation and ventilation was investigated in acute hypotonia after acetylcholin injection. The tonus of respiratory muscles was assessed by measuring the circumference of chest and abdomen. Changes of volume of the body cavities were examined by body plethysmography. A significant relation between the increase of circumferences of the chest and abdomen and increase of lung volume was registered. The authors conclude, that the tonus of the respiratory muscles determines the volume of chest and abdomen and influences the distribution of blood.", "PMID": 960768}
{"id": "pubmed23n0032_4030", "title": "[Disorders of breathing and following gas embolism of the CNS in rats (author's transl)].", "content": "We could produce an acute respiratory insufficiency after injection of O2, CO2 or N2 into the arteria carotis. This resulted in apnea, increase of blood pressure, changes of respiratory rate and coma. The paralysis of the respiratory centre was reversible in some cases by artificial ventilation.", "contents": "[Disorders of breathing and following gas embolism of the CNS in rats (author's transl)]. We could produce an acute respiratory insufficiency after injection of O2, CO2 or N2 into the arteria carotis. This resulted in apnea, increase of blood pressure, changes of respiratory rate and coma. The paralysis of the respiratory centre was reversible in some cases by artificial ventilation.", "PMID": 960769}
{"id": "pubmed23n0032_4031", "title": "[Animal models of chronic lung diseases (author's transl)].", "content": "General aspects of experimental work modelling chronic lung diseases are described starting from experimental lung emphysema and silicosis in rats. Bodyplethysmography was used for the evaluation of the respiratory functions.", "contents": "[Animal models of chronic lung diseases (author's transl)]. General aspects of experimental work modelling chronic lung diseases are described starting from experimental lung emphysema and silicosis in rats. Bodyplethysmography was used for the evaluation of the respiratory functions.", "PMID": 960770}
{"id": "pubmed23n0032_4032", "title": "[On the epidemiology of recurrent and chronic brochopulmonary diseases-an examination of about 18000 children. 2. Investigations in the district of Quedlinburg (author's transl)].", "content": "Report of an epidemiological study in the district of Quedlinburg. This district comprises parts of the mountains of the Harz and an area in front of the Mountains. 4544 children were examined in the age-groups 5.-6., 8.-9., 11.-12. and 14.-15. years of life. In 3,4% of all children a chronic or recurrent nonspecific lung disease was discovered (in 2,5% a recurrent bronchitis, in 0,48% a chronic bronchitis and in 0,24% bronchial asthma). With increasing age the number of diseased children decreases. Differences in the frequency of the diseases between boys and girls could not be found. There were no differences in the rate of the diseases between the towns with somewhat higher degree of air pollution (Quedlinburg and Thale) and the other parts of the district. The frequency of bronchitis however was significantly higher in the mountains of the Hartz than in the lower situated territory, perhaps due to the rougher climate in the mountains.", "contents": "[On the epidemiology of recurrent and chronic brochopulmonary diseases-an examination of about 18000 children. 2. Investigations in the district of Quedlinburg (author's transl)]. Report of an epidemiological study in the district of Quedlinburg. This district comprises parts of the mountains of the Harz and an area in front of the Mountains. 4544 children were examined in the age-groups 5.-6., 8.-9., 11.-12. and 14.-15. years of life. In 3,4% of all children a chronic or recurrent nonspecific lung disease was discovered (in 2,5% a recurrent bronchitis, in 0,48% a chronic bronchitis and in 0,24% bronchial asthma). With increasing age the number of diseased children decreases. Differences in the frequency of the diseases between boys and girls could not be found. There were no differences in the rate of the diseases between the towns with somewhat higher degree of air pollution (Quedlinburg and Thale) and the other parts of the district. The frequency of bronchitis however was significantly higher in the mountains of the Hartz than in the lower situated territory, perhaps due to the rougher climate in the mountains.", "PMID": 960771}
{"id": "pubmed23n0032_4033", "title": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 3. Investigation in the district of Merseburg (author's transl)].", "content": "In the district of Merseburg, characterized by a very high degree of air pollution, we have examined 4445 children of the following 4 age-groups 5.-6., 8.-9., 11.-12., and 14.-16. years of life. As a result we found 3,04% of them suffering from recurrent bronchitis, 0,34% from chronic bronchitis and 0,43% from bronchial asthma. The frequency of morbidity on recurrent and chronic bronchitis lowered with increasing age of the children. In asthma, however, this relation did not exist. There were no differences of the frequency of chronic or recurrent bronchitis between the two sexes, but boys suffered much more often from asthma than girls. In places with a high degree of air pollution the frequency of morbidity was significantly higher than in places with somewhat lower air pollution.", "contents": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 3. Investigation in the district of Merseburg (author's transl)]. In the district of Merseburg, characterized by a very high degree of air pollution, we have examined 4445 children of the following 4 age-groups 5.-6., 8.-9., 11.-12., and 14.-16. years of life. As a result we found 3,04% of them suffering from recurrent bronchitis, 0,34% from chronic bronchitis and 0,43% from bronchial asthma. The frequency of morbidity on recurrent and chronic bronchitis lowered with increasing age of the children. In asthma, however, this relation did not exist. There were no differences of the frequency of chronic or recurrent bronchitis between the two sexes, but boys suffered much more often from asthma than girls. In places with a high degree of air pollution the frequency of morbidity was significantly higher than in places with somewhat lower air pollution.", "PMID": 960772}
{"id": "pubmed23n0032_4034", "title": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 4. Investigation in the cities of Halle and Halle-Neustadt (author's transl)].", "content": "In Halle, an old city, and Halle-Neustadt, a town built in the past 10 years, our investigation was performed on 4418 children. We ascertained chronic or recurrent nonspecific bronchopulmonary diseases in 3,1% of them. 2,4% suffered from recurrent bronchitis, 0,4% from chronic bronchitis and 0,3% from bronchial asthma. Significant differences in the morbidity between the sexes were found only in asthma: boys were fallen ill twice often than girls. The frequency of morbidity lowered with increasing age significantly. Between Halle and Halle-Neustadt significant differences existed: in the old parts of Halle (especially in the centre, the eastern and southern districts) there were much more sick children than in the newly-built town.", "contents": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 4. Investigation in the cities of Halle and Halle-Neustadt (author's transl)]. In Halle, an old city, and Halle-Neustadt, a town built in the past 10 years, our investigation was performed on 4418 children. We ascertained chronic or recurrent nonspecific bronchopulmonary diseases in 3,1% of them. 2,4% suffered from recurrent bronchitis, 0,4% from chronic bronchitis and 0,3% from bronchial asthma. Significant differences in the morbidity between the sexes were found only in asthma: boys were fallen ill twice often than girls. The frequency of morbidity lowered with increasing age significantly. Between Halle and Halle-Neustadt significant differences existed: in the old parts of Halle (especially in the centre, the eastern and southern districts) there were much more sick children than in the newly-built town.", "PMID": 960773}
{"id": "pubmed23n0032_4035", "title": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 5. Investigations in Wolfen and Bitterfeld (author's transl)].", "content": "Bitterfeld and Wolfen are towns with extremely polluted air, due to important plants of the chemical industry. In two different actions we examined 3634 children of four different groups of age. As a result we found 7,8% of them (281 children) suffering from chronic or recurrent nonspecific bronchopulmonary diseases (7,0% suffering from recurrent or chronic bronchitis, and 0,8% from bronchial asthma). The rate of morbidity was the same in both towns. It was significantly higher than in the other districts examined by us (Halle, Merseburg, Quedlinburg). In contrast to the other districts we found in Bitterfeld and Wolfen only an insignificant decrease of the morbity rate with increasing age of the children.", "contents": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 5. Investigations in Wolfen and Bitterfeld (author's transl)]. Bitterfeld and Wolfen are towns with extremely polluted air, due to important plants of the chemical industry. In two different actions we examined 3634 children of four different groups of age. As a result we found 7,8% of them (281 children) suffering from chronic or recurrent nonspecific bronchopulmonary diseases (7,0% suffering from recurrent or chronic bronchitis, and 0,8% from bronchial asthma). The rate of morbidity was the same in both towns. It was significantly higher than in the other districts examined by us (Halle, Merseburg, Quedlinburg). In contrast to the other districts we found in Bitterfeld and Wolfen only an insignificant decrease of the morbity rate with increasing age of the children.", "PMID": 960774}
{"id": "pubmed23n0032_4036", "title": "[Frequency of chronic bronchitis-results of epidemiologic research in the GDR (author's transl)].", "content": "The frequency of chronic bronchitis among 33000 persons, selected by randomization, between 35 and 64 years of age, living in 4 large cities and 24 selected districts with different air pollution levels in GDR was investigated. A chronic bronchitis questionnaire and measurement of FVC, FEV1, PO2 were used to test each person, partially broncho-provocation-tests with histamin aerosols were performed. According to this investigation 6% of the men and 2.8% of the women suffered from CNSLD. In this group of diseased persons 50% showed obstructive disturbances of ventilation, 20% signs of decreased physical fitness due to respiratory disorders and 10% a manifested respiratory insufficiency.", "contents": "[Frequency of chronic bronchitis-results of epidemiologic research in the GDR (author's transl)]. The frequency of chronic bronchitis among 33000 persons, selected by randomization, between 35 and 64 years of age, living in 4 large cities and 24 selected districts with different air pollution levels in GDR was investigated. A chronic bronchitis questionnaire and measurement of FVC, FEV1, PO2 were used to test each person, partially broncho-provocation-tests with histamin aerosols were performed. According to this investigation 6% of the men and 2.8% of the women suffered from CNSLD. In this group of diseased persons 50% showed obstructive disturbances of ventilation, 20% signs of decreased physical fitness due to respiratory disorders and 10% a manifested respiratory insufficiency.", "PMID": 960776}
{"id": "pubmed23n0032_4037", "title": "Respiratory symptoms and lung function disturbances in a polluted area of the Netherlands, compared with a rural area.", "content": "In an epidemiological field survey of CNSLD 1800 men and women aged 15-64 were studied in a rural area and an industrial area. In each person a standardized interview, lung function tests, cardiac examinations, analysis of the phlegm and chest X-ray were performed. The interview conducted with the standard questionnaire gives very good information regarding chronic effects of smoking and atmospheric pollution. Spirometry seems to be a sensitive method for registering an acute disturbances caused by air pollution.", "contents": "Respiratory symptoms and lung function disturbances in a polluted area of the Netherlands, compared with a rural area. In an epidemiological field survey of CNSLD 1800 men and women aged 15-64 were studied in a rural area and an industrial area. In each person a standardized interview, lung function tests, cardiac examinations, analysis of the phlegm and chest X-ray were performed. The interview conducted with the standard questionnaire gives very good information regarding chronic effects of smoking and atmospheric pollution. Spirometry seems to be a sensitive method for registering an acute disturbances caused by air pollution.", "PMID": 960777}
{"id": "pubmed23n0032_4038", "title": "[The 2nd decree on prevention and control of tuberculosis; new tasks and perspectives for pneumonology in GDR (AUTHOR'S TRANSL)].", "content": "At July 1st the tasks of the former chest clinics, now integrated into the polyclinical system as polyclinical departments for respiratory diseases and tuberculosis are fixed and declared by decree and 3 regulations in the field of pneumology. This new orientation is part of the general development of socialist public health system concerning all reactes of health care. The unity of prevention, diagnostics, treatment and surveillance (metaphylaxis) will be emphasized in all respects. The 3 regulations concern the tasks of the polyclinical departments for respiratory diseases and tuberculosis, mass x-ray examinations and BCG-vaccination.", "contents": "[The 2nd decree on prevention and control of tuberculosis; new tasks and perspectives for pneumonology in GDR (AUTHOR'S TRANSL)]. At July 1st the tasks of the former chest clinics, now integrated into the polyclinical system as polyclinical departments for respiratory diseases and tuberculosis are fixed and declared by decree and 3 regulations in the field of pneumology. This new orientation is part of the general development of socialist public health system concerning all reactes of health care. The unity of prevention, diagnostics, treatment and surveillance (metaphylaxis) will be emphasized in all respects. The 3 regulations concern the tasks of the polyclinical departments for respiratory diseases and tuberculosis, mass x-ray examinations and BCG-vaccination.", "PMID": 960778}
{"id": "pubmed23n0032_4039", "title": "[Frequency of disturbances of pulmonary function in 4 cities of the GDR (author's transl)].", "content": "8369 men aged 35-64 were surveyed in 4 cities. The levels of FVC, FEV, pO2a were examined in connection with the standard questionnaire. The problems of lung function testing in epidemiological studies are discussed.", "contents": "[Frequency of disturbances of pulmonary function in 4 cities of the GDR (author's transl)]. 8369 men aged 35-64 were surveyed in 4 cities. The levels of FVC, FEV, pO2a were examined in connection with the standard questionnaire. The problems of lung function testing in epidemiological studies are discussed.", "PMID": 960779}
{"id": "pubmed23n0032_4040", "title": "[Functional diagnosis of CNSLD in epidemiological surveys-results of the population study in Halle 1974 (author's transl)].", "content": "The problems of lung function testing methods in epidemiological studies were assessed in a study population of 853 men aged 40-70. The Helium-rebreathing-method, the X-ray densitometry, the oxygen-method were evaluated for the estimation of the residual volume in epidemiological conditions. The Pneumotest was proved for measurement of unequal ventilation. The necessity of reference values for these methods is discussed.", "contents": "[Functional diagnosis of CNSLD in epidemiological surveys-results of the population study in Halle 1974 (author's transl)]. The problems of lung function testing methods in epidemiological studies were assessed in a study population of 853 men aged 40-70. The Helium-rebreathing-method, the X-ray densitometry, the oxygen-method were evaluated for the estimation of the residual volume in epidemiological conditions. The Pneumotest was proved for measurement of unequal ventilation. The necessity of reference values for these methods is discussed.", "PMID": 960780}
{"id": "pubmed23n0032_4041", "title": "[Rhinomanometric findings during brine aerosol inhalations (author's transl)].", "content": "Brine aerosol inhalations cause reactions of the nasal mucous membrane and the submucousal vascular bed as is shown by rhinomanometric examinations of healthy persons and patients suffering from various kinds of rhinitis. Changes in the resistance as a whole differ from those in partial resistances. The reactions are depending on the form of inhalation, the spectrum and concentration of the aerosol, climatic parameters and the pathophysiologic condition of the nasal mucosa.", "contents": "[Rhinomanometric findings during brine aerosol inhalations (author's transl)]. Brine aerosol inhalations cause reactions of the nasal mucous membrane and the submucousal vascular bed as is shown by rhinomanometric examinations of healthy persons and patients suffering from various kinds of rhinitis. Changes in the resistance as a whole differ from those in partial resistances. The reactions are depending on the form of inhalation, the spectrum and concentration of the aerosol, climatic parameters and the pathophysiologic condition of the nasal mucosa.", "PMID": 960781}
{"id": "pubmed23n0032_4042", "title": "[Clinical treatment of tracheoesophageal fistulas due to foreign bodies (author's transl)].", "content": "Swallowed foreign bodies, fixed in the esophagus, are not uncommon in childhood. Their symptoms, complications, diagnosis and treatment are discussed. An expectant attitude is justified in regard to smaller fistulas and in big fistulas due to foreign bodies up to the remission of the localized inflammatory reaction. The present surgical methods are reviewed in the light of recent literature and a case study of a child cured by segmental resection of the trachea.", "contents": "[Clinical treatment of tracheoesophageal fistulas due to foreign bodies (author's transl)]. Swallowed foreign bodies, fixed in the esophagus, are not uncommon in childhood. Their symptoms, complications, diagnosis and treatment are discussed. An expectant attitude is justified in regard to smaller fistulas and in big fistulas due to foreign bodies up to the remission of the localized inflammatory reaction. The present surgical methods are reviewed in the light of recent literature and a case study of a child cured by segmental resection of the trachea.", "PMID": 960782}
{"id": "pubmed23n0032_4043", "title": "[The main principles in the organization of pulmonary care in USSR (author's transl)].", "content": "It is reported on the systematic development and on the graduated distribution of tasks in the field of pneumonology in the different stages of medical care. The greatest importance has the physician in the basic care, the therapeut in the territory. He is advised in diagnostic and therapy by the pneumonologist in the special departments of the polyclinics. These will get the assistance of the special hospital institutions of the districts and countries. Connections to the antituberculosis network exist at several places of the country. They are favoured in future development.", "contents": "[The main principles in the organization of pulmonary care in USSR (author's transl)]. It is reported on the systematic development and on the graduated distribution of tasks in the field of pneumonology in the different stages of medical care. The greatest importance has the physician in the basic care, the therapeut in the territory. He is advised in diagnostic and therapy by the pneumonologist in the special departments of the polyclinics. These will get the assistance of the special hospital institutions of the districts and countries. Connections to the antituberculosis network exist at several places of the country. They are favoured in future development.", "PMID": 960783}
{"id": "pubmed23n0032_4044", "title": "[Experiences with the mediastinoscopy for the diagnostics of silicosis (author's transl)].", "content": "122 patients, suspicious for silicoses, with a history of silicogen dust exposure, radiological changes and restricted lung function values were examined by mediastinal biopsy for securing the diagnosis. In 82 patients (67,2%) a silicosis could be confirmed by histological examinations of the mediastinal lymph nodes. A close association between silicosis in the lungs and in the lymph nodes is empirically suggested. If the histological findings were in compliance with suspicious radiological changes and patients history, silicosis was acknowledged as an occupational disease. In patients with beginning silicotic changes in the peripheral two upper lung fields associated with an hilar hyperplasia, a higher proportion of positive results could be found. The most positive alterations were found in the lymph nodes of the right hilus. The mediastinoscopical findings in other parts proved not to be so yielding. Therefore, in cases with anatomical difficulties the surgical examination should not be extended to other parts.", "contents": "[Experiences with the mediastinoscopy for the diagnostics of silicosis (author's transl)]. 122 patients, suspicious for silicoses, with a history of silicogen dust exposure, radiological changes and restricted lung function values were examined by mediastinal biopsy for securing the diagnosis. In 82 patients (67,2%) a silicosis could be confirmed by histological examinations of the mediastinal lymph nodes. A close association between silicosis in the lungs and in the lymph nodes is empirically suggested. If the histological findings were in compliance with suspicious radiological changes and patients history, silicosis was acknowledged as an occupational disease. In patients with beginning silicotic changes in the peripheral two upper lung fields associated with an hilar hyperplasia, a higher proportion of positive results could be found. The most positive alterations were found in the lymph nodes of the right hilus. The mediastinoscopical findings in other parts proved not to be so yielding. Therefore, in cases with anatomical difficulties the surgical examination should not be extended to other parts.", "PMID": 960784}
{"id": "pubmed23n0032_4045", "title": "[Preventive chemotherapy in persons with silicosis and silicotuberculosis (author's transl)].", "content": "In some countries extensive research is carried out on preventing tuberculous disease by administration of INH. In GDR since about 1960 chemopreventive measures were taken in cases of silicosis and silicotuberculosis too. More than 2000 patients of the Magdeburg Pneumoconiosis Center were followed-up from 1960 to 1969. The high risk of tuberculous disease of 21% in the group of silicosis patients without administration of INH could be reduced to 14% after preventive administration of INH. However, such clear effects were observed only under optimal conditions, that is sufficient dosage, duration and reliability of treatment. On the base of these results and other publications patients with silicosis with and without tuberculous residues should be included into the programmes of preventive chemotherapy of the chest clinics.", "contents": "[Preventive chemotherapy in persons with silicosis and silicotuberculosis (author's transl)]. In some countries extensive research is carried out on preventing tuberculous disease by administration of INH. In GDR since about 1960 chemopreventive measures were taken in cases of silicosis and silicotuberculosis too. More than 2000 patients of the Magdeburg Pneumoconiosis Center were followed-up from 1960 to 1969. The high risk of tuberculous disease of 21% in the group of silicosis patients without administration of INH could be reduced to 14% after preventive administration of INH. However, such clear effects were observed only under optimal conditions, that is sufficient dosage, duration and reliability of treatment. On the base of these results and other publications patients with silicosis with and without tuberculous residues should be included into the programmes of preventive chemotherapy of the chest clinics.", "PMID": 960785}
{"id": "pubmed23n0032_4046", "title": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 1. Planning and organization of the investigation (author's transl)].", "content": "The planning and organization of an epidemiological investigation is reported to find out the frequency of chronic nonspecific lung diseases (CNSLD) in childhood as a whole and in special groups of age. By investigating regions with different air pollution we tried to study the influence of air pollution on the frequency of childhood lung diseases. Therefore, we examined nearly 5000 children in the city of Halle and in the districts of Quedlinburg, Merseburg and Bitterfeld. The children belonged to the age-groups 5.-6., 8.-9., 11.-12. and 14.-15. years of life in nearly the same number. The investigations were performed by different groups of investigators. However, by equal terms of reference and uniform evaluation of the results by two of us the results of the four districts are comparable with each other.", "contents": "[On the epidemiology of recurrent and chronic bronchopulmonary diseases-an examination of about 18000 children. 1. Planning and organization of the investigation (author's transl)]. The planning and organization of an epidemiological investigation is reported to find out the frequency of chronic nonspecific lung diseases (CNSLD) in childhood as a whole and in special groups of age. By investigating regions with different air pollution we tried to study the influence of air pollution on the frequency of childhood lung diseases. Therefore, we examined nearly 5000 children in the city of Halle and in the districts of Quedlinburg, Merseburg and Bitterfeld. The children belonged to the age-groups 5.-6., 8.-9., 11.-12. and 14.-15. years of life in nearly the same number. The investigations were performed by different groups of investigators. However, by equal terms of reference and uniform evaluation of the results by two of us the results of the four districts are comparable with each other.", "PMID": 960786}
{"id": "pubmed23n0032_4047", "title": "Biological effects of transfatty acids.", "content": "Transfatty acids are geometrical isomers of the naturally occurring cis-fatty acids, and their molecular configuration is more like that of the corresponding saturated fatty acid. In view of the large amounts of trans-acids in commercially prepared margarines, metabolic studies with trans-acids have received a great deal of attention. Although there are differences in the absorption and digestion of trans-acids and although considerable amounts are deposited in various tissues, no definite signs of toxicity have been observed. An important finding is the increased requirement for essential fatty acids in animals fed high levels of trans-acids. In recent work carried out in Kummerow's laboratory, aortic lesions were found in swine fed large amounts of trans-acids. Evaluation of the data suggests that factors other than trans-acids, per se, may have been responsible for the occurrence of the lesions. As yet, there does not seem to be any valid reason why trans-acids should be excluded from the diet as long as their intake is associated with a high enough level of essential fatty acids in the diet.", "contents": "Biological effects of transfatty acids. Transfatty acids are geometrical isomers of the naturally occurring cis-fatty acids, and their molecular configuration is more like that of the corresponding saturated fatty acid. In view of the large amounts of trans-acids in commercially prepared margarines, metabolic studies with trans-acids have received a great deal of attention. Although there are differences in the absorption and digestion of trans-acids and although considerable amounts are deposited in various tissues, no definite signs of toxicity have been observed. An important finding is the increased requirement for essential fatty acids in animals fed high levels of trans-acids. In recent work carried out in Kummerow's laboratory, aortic lesions were found in swine fed large amounts of trans-acids. Evaluation of the data suggests that factors other than trans-acids, per se, may have been responsible for the occurrence of the lesions. As yet, there does not seem to be any valid reason why trans-acids should be excluded from the diet as long as their intake is associated with a high enough level of essential fatty acids in the diet.", "PMID": 960788}
{"id": "pubmed23n0032_4048", "title": "[Metabolic effect of coffee and caffeine].", "content": "Coffee and its most important constituent, caffeine, may not only stimulate the function of many organs but also increase the metabolism in the body. These effects require a higher energy production which is mainly obtained from striated muscles by glycogenolysis and from fat tissue by lipolysis. Sutherland and Butcher were able to demonstrate that these degradation processes are primarily caused by an increase of cyclic 3,5-AMP. - In this connection caffeine and other methylxanthines are of special interest because these compounds also increase the intracellular amount of cyclic 3,5-AMP. This effect may be caused by an inhibition of phosphodiesterase, a release of catecholamines with resulting stimulation of adenylcyclase or by competitive inhibition of adenosine. At the present time it cannot be said which of these mechanisms primarily is involved in the in vivo effects of caffeine and other methylxanthines.", "contents": "[Metabolic effect of coffee and caffeine]. Coffee and its most important constituent, caffeine, may not only stimulate the function of many organs but also increase the metabolism in the body. These effects require a higher energy production which is mainly obtained from striated muscles by glycogenolysis and from fat tissue by lipolysis. Sutherland and Butcher were able to demonstrate that these degradation processes are primarily caused by an increase of cyclic 3,5-AMP. - In this connection caffeine and other methylxanthines are of special interest because these compounds also increase the intracellular amount of cyclic 3,5-AMP. This effect may be caused by an inhibition of phosphodiesterase, a release of catecholamines with resulting stimulation of adenylcyclase or by competitive inhibition of adenosine. At the present time it cannot be said which of these mechanisms primarily is involved in the in vivo effects of caffeine and other methylxanthines.", "PMID": 960787}
{"id": "pubmed23n0032_4049", "title": "Amido black dye binding of alfalfa and forage legume protein.", "content": "Although dye binding has been suggested for the determination of alfalfa protein content, a standard method has not been advanced. More data are necessary on the conditions required for the dye-protein reaction. Reaction times of 8, 12 and 24 hours and reaction temperatures of 50, 55, 60 and 65 degrees C were selected to obtain dye binding values (DBV) with the Foss Pro-Milk apparatus. A correlation of 0.88 with macro-Kjeldahl values was obtained. 60 degrees C and 24 hours reaction time can be considered conditions yielding maximum DBV. It is suggested to develop a standard method for the routine measurement of forage legume content.", "contents": "Amido black dye binding of alfalfa and forage legume protein. Although dye binding has been suggested for the determination of alfalfa protein content, a standard method has not been advanced. More data are necessary on the conditions required for the dye-protein reaction. Reaction times of 8, 12 and 24 hours and reaction temperatures of 50, 55, 60 and 65 degrees C were selected to obtain dye binding values (DBV) with the Foss Pro-Milk apparatus. A correlation of 0.88 with macro-Kjeldahl values was obtained. 60 degrees C and 24 hours reaction time can be considered conditions yielding maximum DBV. It is suggested to develop a standard method for the routine measurement of forage legume content.", "PMID": 960789}
{"id": "pubmed23n0032_4050", "title": "[Utilization of xylitol in the isolation-perfused myocardium of the rat].", "content": "1. In the rat heart muscle, only 3 to 4% of the oxygen-consumption can be referred to xylitol utilization. With xylitol as only substrate the heart is working under substrate deficiency conditions. 2. Diabetes mellitus does not improve the xylitol utilization in the rat heart muscle. 3. The data are compared with the activities of xylitol degrading enzymes in the heart muscle.", "contents": "[Utilization of xylitol in the isolation-perfused myocardium of the rat]. 1. In the rat heart muscle, only 3 to 4% of the oxygen-consumption can be referred to xylitol utilization. With xylitol as only substrate the heart is working under substrate deficiency conditions. 2. Diabetes mellitus does not improve the xylitol utilization in the rat heart muscle. 3. The data are compared with the activities of xylitol degrading enzymes in the heart muscle.", "PMID": 960791}
{"id": "pubmed23n0032_4051", "title": "[Ultrasonic determination of subcutaneous adipose tissue and body fat].", "content": "We measured with the ultrasonic reflexion method the breadth of the subcutaneous adipose tissue at the following anatomical sites: subscapulare, triceps and on the lateral margin of the m. pectoralis on the chest. The percentual body fat can be calculated from the formula: % body fat = 22 - log / 2 - sum of the breadths on the three sites / -20.5 If we measure also the breadth on the abdominal point the % body fat is 22 - log / 1.5 - sum on the four sites / -20.5. The so calculated numerical results are with the skinfold measurements in best correlation.", "contents": "[Ultrasonic determination of subcutaneous adipose tissue and body fat]. We measured with the ultrasonic reflexion method the breadth of the subcutaneous adipose tissue at the following anatomical sites: subscapulare, triceps and on the lateral margin of the m. pectoralis on the chest. The percentual body fat can be calculated from the formula: % body fat = 22 - log / 2 - sum of the breadths on the three sites / -20.5 If we measure also the breadth on the abdominal point the % body fat is 22 - log / 1.5 - sum on the four sites / -20.5. The so calculated numerical results are with the skinfold measurements in best correlation.", "PMID": 960792}
{"id": "pubmed23n0032_4052", "title": "[Teratogenic and mutagenic studies with caffeine in the animal experiment].", "content": "Principles of teratogenic and mutagenic actions are defined. The recent experimental studies with laboratory animals, and our investigations with caffeine-sodium benzoicum and with soluble coffee in pregnant rats and mice showed no teratogenicity. The results are compared with specific teratogenic effects of cytostatic agents. A teratogenicity of caffeine can be excluded, a mutagenicity in animal experiments can also not be proved.", "contents": "[Teratogenic and mutagenic studies with caffeine in the animal experiment]. Principles of teratogenic and mutagenic actions are defined. The recent experimental studies with laboratory animals, and our investigations with caffeine-sodium benzoicum and with soluble coffee in pregnant rats and mice showed no teratogenicity. The results are compared with specific teratogenic effects of cytostatic agents. A teratogenicity of caffeine can be excluded, a mutagenicity in animal experiments can also not be proved.", "PMID": 960793}
{"id": "pubmed23n0032_4053", "title": "[Multichannel EEG spectral analysis of the caffeine effect].", "content": "By means of multichannel Eeg spectral analysis the central effects of caffeine have been investigated during a controlled factorial study with 9 male volunteers. The effects of coffee and caffeine solution with equal concentration, decaffeinated coffee and hot water have been compared on the basis of analysis of variance. The factorial experimental design implemented a randomization concerning the sequence of substances as well as days of week. The principal findings are following: coffee and caffeine solution of equal concentration on the one hand cannot be readily distinguished concerning their Eeg effects, on the other hand the decaffeinated coffee and hot water exhibit very similar Eeg effects. Very marked differences, however, have been found between the two groups of substances. Coffee and caffeine solution both produce a remarkable, highly significant reduction of theta-amplitude, dominant theta-frequency and theta-frequency-variation. The alterations within the alpha range are mainly increase of dominant frequency and decrease of amplitude. These findings may be interpreted as signs of remarkable activation and elevation of vigilance. It should be pointed out that the Eeg effects of caffeine, as well as those of other compounds with central effects, are characterized by topographically different effects. These are not only quantitative, but partially qualitative differences.", "contents": "[Multichannel EEG spectral analysis of the caffeine effect]. By means of multichannel Eeg spectral analysis the central effects of caffeine have been investigated during a controlled factorial study with 9 male volunteers. The effects of coffee and caffeine solution with equal concentration, decaffeinated coffee and hot water have been compared on the basis of analysis of variance. The factorial experimental design implemented a randomization concerning the sequence of substances as well as days of week. The principal findings are following: coffee and caffeine solution of equal concentration on the one hand cannot be readily distinguished concerning their Eeg effects, on the other hand the decaffeinated coffee and hot water exhibit very similar Eeg effects. Very marked differences, however, have been found between the two groups of substances. Coffee and caffeine solution both produce a remarkable, highly significant reduction of theta-amplitude, dominant theta-frequency and theta-frequency-variation. The alterations within the alpha range are mainly increase of dominant frequency and decrease of amplitude. These findings may be interpreted as signs of remarkable activation and elevation of vigilance. It should be pointed out that the Eeg effects of caffeine, as well as those of other compounds with central effects, are characterized by topographically different effects. These are not only quantitative, but partially qualitative differences.", "PMID": 960794}
{"id": "pubmed23n0032_4054", "title": "[Metabolism studies with coffee and caffeine in healthy subjects, diabetics and patients with liver disease].", "content": "Peroral application of caffeine or theophylline causes a significant increase of the free fatty acids and the free glycerol of the serum; blood sugar and cholesterol are not influenced. FFA and free glycerol decrease during the day by food intake, blood sugar and triglycerides rise. The ingestion of coffee (1-3 cups) does not influence fat and carbohydrate metabolism. To induce a significant increase of lipolysis and of serum triglyceride levels high caffeine doses (1000 mg) are required. Summarizing the data presented, the authors conclude that coffee intake (at dosages compatible with normal nutrition) does not deteriorate glucose tolerance and does not interfere with the fat metabolism. Our findings are confirmed by epidemiologic investigations which have shown that coffee intake is no risk factor for myocardial infarction.", "contents": "[Metabolism studies with coffee and caffeine in healthy subjects, diabetics and patients with liver disease]. Peroral application of caffeine or theophylline causes a significant increase of the free fatty acids and the free glycerol of the serum; blood sugar and cholesterol are not influenced. FFA and free glycerol decrease during the day by food intake, blood sugar and triglycerides rise. The ingestion of coffee (1-3 cups) does not influence fat and carbohydrate metabolism. To induce a significant increase of lipolysis and of serum triglyceride levels high caffeine doses (1000 mg) are required. Summarizing the data presented, the authors conclude that coffee intake (at dosages compatible with normal nutrition) does not deteriorate glucose tolerance and does not interfere with the fat metabolism. Our findings are confirmed by epidemiologic investigations which have shown that coffee intake is no risk factor for myocardial infarction.", "PMID": 960795}
{"id": "pubmed23n0032_4055", "title": "[Psychologic study of experienced coffee effect].", "content": "In dealing with the psychological effects of coffee a distinction is drawn between three components of the subjective or experimential effect: the primary effect, which relates to the perception of the coffee beverage; the secondary effect, which stems from the ergotropic effect of coffee; and the tertiary effect, which is due to visceral sensations and which is a major contributor to the experience of digestability and wholesomeness. Results pertaining to these three components, their interaction, and their correlation with physiological and behavioral effects of coffee are discussed. The variability of research findings relating to the secondary effect is explained on the basis of the psychological activation theory. Relevant motivational, attitudinal, and moderator effects, which psychological coffee research will have to take into account, are described. Methods already available for psychological coffee research, including suitable techniques of experimentation and measurement, are presented together with three illustrative examples.", "contents": "[Psychologic study of experienced coffee effect]. In dealing with the psychological effects of coffee a distinction is drawn between three components of the subjective or experimential effect: the primary effect, which relates to the perception of the coffee beverage; the secondary effect, which stems from the ergotropic effect of coffee; and the tertiary effect, which is due to visceral sensations and which is a major contributor to the experience of digestability and wholesomeness. Results pertaining to these three components, their interaction, and their correlation with physiological and behavioral effects of coffee are discussed. The variability of research findings relating to the secondary effect is explained on the basis of the psychological activation theory. Relevant motivational, attitudinal, and moderator effects, which psychological coffee research will have to take into account, are described. Methods already available for psychological coffee research, including suitable techniques of experimentation and measurement, are presented together with three illustrative examples.", "PMID": 960796}
{"id": "pubmed23n0032_4056", "title": "[Transitional symptoms, adverse effects and incidents in hormone-substitution therapy].", "content": "Patients underlying the permanent endocrine substitution need a particular control and a competent conduction on account of their endangering by intercurrent events. Highly specialised knowledge of the physician and intensive collaboration of the patient from this reciprocity lead to essential aspects of the prophylaxis of the crisis-like exacerbations exhibited in detail. The optimum substitution is supplemented by issuing information and emergency cards. When the patient possesses such cards they will become of decisive importance in an urgent therapy necessary outside the controlling facility.", "contents": "[Transitional symptoms, adverse effects and incidents in hormone-substitution therapy]. Patients underlying the permanent endocrine substitution need a particular control and a competent conduction on account of their endangering by intercurrent events. Highly specialised knowledge of the physician and intensive collaboration of the patient from this reciprocity lead to essential aspects of the prophylaxis of the crisis-like exacerbations exhibited in detail. The optimum substitution is supplemented by issuing information and emergency cards. When the patient possesses such cards they will become of decisive importance in an urgent therapy necessary outside the controlling facility.", "PMID": 960851}
{"id": "pubmed23n0032_4057", "title": "[Objectivation of the cardiac insufficiency load index--a comparison between spiroergometry and stretch-index].", "content": "It was shown that reduction in exercise capacity because of latent heart insufficiency could be significantly demonstrated by the so-called pre-ejection index PI, measured under conditions of rest. On the other hand, normals did not show any significant interrelation between PI and parameters related to exercise capacity, obtained by spiroergometric measurements. The pre-ejection index PI is a non-invasive parameter which compares the pre-ejection period in upright and supine posture. Since spiroergometric measurements could not be made under vita-maxima conditions, PI values were compared with parameters of submaximal exercise. Exercise was performed on the bicycle ergometer, with oxygen consumption and heart rate being recorded. Measuring results also revealed that by means of the pre-ejection index the necessity and effectiveness of glycoside medication may be objectively defined. Measuring techniques are described and statistically processed data are presented.", "contents": "[Objectivation of the cardiac insufficiency load index--a comparison between spiroergometry and stretch-index]. It was shown that reduction in exercise capacity because of latent heart insufficiency could be significantly demonstrated by the so-called pre-ejection index PI, measured under conditions of rest. On the other hand, normals did not show any significant interrelation between PI and parameters related to exercise capacity, obtained by spiroergometric measurements. The pre-ejection index PI is a non-invasive parameter which compares the pre-ejection period in upright and supine posture. Since spiroergometric measurements could not be made under vita-maxima conditions, PI values were compared with parameters of submaximal exercise. Exercise was performed on the bicycle ergometer, with oxygen consumption and heart rate being recorded. Measuring results also revealed that by means of the pre-ejection index the necessity and effectiveness of glycoside medication may be objectively defined. Measuring techniques are described and statistically processed data are presented.", "PMID": 960852}
{"id": "pubmed23n0032_4058", "title": "[Measurement of kidney concentrating ability in children with nonrenal glycosuria].", "content": "In non-renal (diabetic) glucosuria we did not find any statistically real relations between the concentration of glucose in the urine and cryoscopically measured osmolality in children with healthy kidneys. The close negative correlation of the conductance of the urine to the concentration of glucose is not only to be explained by changes of the viscosity, but is an expression of an increased re-absorption of sodium as a result of a compensatory hyperaldosteronism. In renal insufficiency the electrolytic conductibility of the urine is lower than the borderline area of the normal, even when under influence of the glucose excretion the osmolality of the urine is still to be found normal. Thus also on the conditions of a considerable glucosuria we can further judge the concentrating ability of the kidney in diabetes mellitus with the help of the measurement of the conductance of the urine.", "contents": "[Measurement of kidney concentrating ability in children with nonrenal glycosuria]. In non-renal (diabetic) glucosuria we did not find any statistically real relations between the concentration of glucose in the urine and cryoscopically measured osmolality in children with healthy kidneys. The close negative correlation of the conductance of the urine to the concentration of glucose is not only to be explained by changes of the viscosity, but is an expression of an increased re-absorption of sodium as a result of a compensatory hyperaldosteronism. In renal insufficiency the electrolytic conductibility of the urine is lower than the borderline area of the normal, even when under influence of the glucose excretion the osmolality of the urine is still to be found normal. Thus also on the conditions of a considerable glucosuria we can further judge the concentrating ability of the kidney in diabetes mellitus with the help of the measurement of the conductance of the urine.", "PMID": 960853}
{"id": "pubmed23n0032_4059", "title": "[Problem of catheter-embolism following subclavian puncture].", "content": "The catheterisation into the subclavian vein is important in the intensive therapy and for the prolonged parenteral nutrition. Numerous complications are described. In 300 catheterisations into the subclavian vein we observed 3 damages of catheters. The causes damages of catheters are investigated with the help of experiments. Recommendations to the prevention of the damage and the embolism of the subclavian catheters are given.", "contents": "[Problem of catheter-embolism following subclavian puncture]. The catheterisation into the subclavian vein is important in the intensive therapy and for the prolonged parenteral nutrition. Numerous complications are described. In 300 catheterisations into the subclavian vein we observed 3 damages of catheters. The causes damages of catheters are investigated with the help of experiments. Recommendations to the prevention of the damage and the embolism of the subclavian catheters are given.", "PMID": 960854}
{"id": "pubmed23n0032_4060", "title": "[X-ray findings in bones of patients with chronic renal insufficiency under chronic hemodialysis program].", "content": "In the chronic dialysis program, taking into consideration the duration of the dialysis, we sought in 25 patients (average age 34 years) for the radiological signs of the uraemic osteopathy, of the ectosteal calcification and the vascular calcification of the type M\u00f6nkeberg. In our patients a radiologically visible affection was to be proved at a duration of the dialysis of more than 3 months. Size and kind of the alterations depended on the age of the patients. In patients younger than 40 years we established an accumulation of isolated soft tissue or osseous processes, in patients older than 40 years the combination of multilocular osseous changes with calcification of the soft tissue or vascular calcium was of interest. Calcifications of the vessels of type M\u00f6nkeberg we could not find in patients younger than 40 years also at a duration of the dialysis of more than 1 year. This work shall be starting point of further observations on patients with chronic renal insufficiency during the course of dialysis, especially after changing of the calcium content of the dialysis solution.", "contents": "[X-ray findings in bones of patients with chronic renal insufficiency under chronic hemodialysis program]. In the chronic dialysis program, taking into consideration the duration of the dialysis, we sought in 25 patients (average age 34 years) for the radiological signs of the uraemic osteopathy, of the ectosteal calcification and the vascular calcification of the type M\u00f6nkeberg. In our patients a radiologically visible affection was to be proved at a duration of the dialysis of more than 3 months. Size and kind of the alterations depended on the age of the patients. In patients younger than 40 years we established an accumulation of isolated soft tissue or osseous processes, in patients older than 40 years the combination of multilocular osseous changes with calcification of the soft tissue or vascular calcium was of interest. Calcifications of the vessels of type M\u00f6nkeberg we could not find in patients younger than 40 years also at a duration of the dialysis of more than 1 year. This work shall be starting point of further observations on patients with chronic renal insufficiency during the course of dialysis, especially after changing of the calcium content of the dialysis solution.", "PMID": 960855}
{"id": "pubmed23n0032_4061", "title": "[Differentiation of organic heart diseases and functionally conditioned cardiovascular disorders with special reference to heart-ache].", "content": "Functional cardiovascular disturbances are very often in daily practice. Making of the diagnosis is possible by the exclusion of organic heart diseases, the behaviour and the special personal structure of the patient. Functional cardiovascular disturbances may manifest themselves as disturbances of the cardiac rhythm, circulatory dysregulations and so-called heart-aches, in which cases most different variations are possible. They coincide isolately, but also additionally with organic heart diseases, which emphasizes the necessity of thorough examinations. In detail the author enters into heart-ache and its differentiation in concomitant unspecific changes of the final part in the ECG.", "contents": "[Differentiation of organic heart diseases and functionally conditioned cardiovascular disorders with special reference to heart-ache]. Functional cardiovascular disturbances are very often in daily practice. Making of the diagnosis is possible by the exclusion of organic heart diseases, the behaviour and the special personal structure of the patient. Functional cardiovascular disturbances may manifest themselves as disturbances of the cardiac rhythm, circulatory dysregulations and so-called heart-aches, in which cases most different variations are possible. They coincide isolately, but also additionally with organic heart diseases, which emphasizes the necessity of thorough examinations. In detail the author enters into heart-ache and its differentiation in concomitant unspecific changes of the final part in the ECG.", "PMID": 960856}
{"id": "pubmed23n0032_4062", "title": "[Significance of mental factors in paroxysmal ventricular tachycardias].", "content": "In this paper is reported on two cases of paroxysmal ventricular tachycardia. The clinical and paraclinical examinations did not reveal any organic heart disease, the psychological one, however, found a very severe neurotic personality disorder. Antiarrhythmic drugs were ineffective in both cases. Meanwhile one female patient went through a brief psychotherapy which dismissed the arrhythmia.", "contents": "[Significance of mental factors in paroxysmal ventricular tachycardias]. In this paper is reported on two cases of paroxysmal ventricular tachycardia. The clinical and paraclinical examinations did not reveal any organic heart disease, the psychological one, however, found a very severe neurotic personality disorder. Antiarrhythmic drugs were ineffective in both cases. Meanwhile one female patient went through a brief psychotherapy which dismissed the arrhythmia.", "PMID": 960857}
{"id": "pubmed23n0032_4063", "title": "[Simultaneous incidence of chronic dissecans aortic aneurysm and bidirectional tachydardia].", "content": "In a 68-year-old patient during a therapy with digitalis and saluretics with maintenance doses a severe cardiac insufficiency develops. After intravenous injection of K-strophantoside temporarily a bidirectional tachycardia developed which enforced a discontinuation of the K-strophantoside therapy. A deterioration of the cardiac insufficiency caused the resumption of the strophantine treatment under the protection of novocamide (procainamide) and panangine (magnesium asparagin. anhydr. and calcium asparagin. anhydr.) without reappearance of a bidirectional tachycardia. The patient died of a severe cardiovascular insufficiency. If the bidirectional tachycardia appears in severe cardiac insufficiency a continuation of the treatment with K-strophantoside can be tried under the protection of antiarrhythmic drugs.", "contents": "[Simultaneous incidence of chronic dissecans aortic aneurysm and bidirectional tachydardia]. In a 68-year-old patient during a therapy with digitalis and saluretics with maintenance doses a severe cardiac insufficiency develops. After intravenous injection of K-strophantoside temporarily a bidirectional tachycardia developed which enforced a discontinuation of the K-strophantoside therapy. A deterioration of the cardiac insufficiency caused the resumption of the strophantine treatment under the protection of novocamide (procainamide) and panangine (magnesium asparagin. anhydr. and calcium asparagin. anhydr.) without reappearance of a bidirectional tachycardia. The patient died of a severe cardiovascular insufficiency. If the bidirectional tachycardia appears in severe cardiac insufficiency a continuation of the treatment with K-strophantoside can be tried under the protection of antiarrhythmic drugs.", "PMID": 960858}
{"id": "pubmed23n0032_4064", "title": "[Problems of idiopathic congestive cardiomyopathy].", "content": "It is reported on the course of an idiopathic congestive cardiomyopathy in a younger man which could be observed during three years. After a longer period free of symptoms acutely appeared the signs of a myocardial failure, in which case disturbances of cardiac rhythm at length caused death. In detail the clinical fingings, pathologo-anatomical changes as well as the differential-diagnostic considerations are discussed, which are important for the demarcation of the other forms of the idiopathic and secondary cardiomyopathies.", "contents": "[Problems of idiopathic congestive cardiomyopathy]. It is reported on the course of an idiopathic congestive cardiomyopathy in a younger man which could be observed during three years. After a longer period free of symptoms acutely appeared the signs of a myocardial failure, in which case disturbances of cardiac rhythm at length caused death. In detail the clinical fingings, pathologo-anatomical changes as well as the differential-diagnostic considerations are discussed, which are important for the demarcation of the other forms of the idiopathic and secondary cardiomyopathies.", "PMID": 960859}
{"id": "pubmed23n0032_4065", "title": "[Quintary hyperparathyroidism].", "content": "The quartary and quintary hyperparathyroidism is observed following a primary hyperparathyroidism, in which the removal of the adenoma of the parathyroid did not lead to a reduction of the process of the disease. The nephropathy which developed during the basic disease and continued to exist after the operation leads to repeated consequences for the mineral metabolism which cause a condition of parathyroidal load and after this again an adenomatous process. The author adopts a definite attitude to the problems of heuristic biochemical parameters and generally to the question of such consecutive reactions.", "contents": "[Quintary hyperparathyroidism]. The quartary and quintary hyperparathyroidism is observed following a primary hyperparathyroidism, in which the removal of the adenoma of the parathyroid did not lead to a reduction of the process of the disease. The nephropathy which developed during the basic disease and continued to exist after the operation leads to repeated consequences for the mineral metabolism which cause a condition of parathyroidal load and after this again an adenomatous process. The author adopts a definite attitude to the problems of heuristic biochemical parameters and generally to the question of such consecutive reactions.", "PMID": 960860}
{"id": "pubmed23n0032_4066", "title": "[Studies on the functional ability of swine liver perfused with human blood in machine recirculation attempt. Experiment design, elimination of indocyanine green, galactose, bilirubin and ammonia, synthesis of urea, bile secretion].", "content": "In 11 machine experiments of recirculation the abilities of survival and functioning of livers of dwarf pigs perfused with human preserved blood were tested with the help of the eliminations of indocyanine green, galactose, bilirubin and ammonia from a closed circulation. Further urea-nitrogen were measured as indicator of the urea synthesis and bile secretion. The perfusion of the animal livers was carried out in 9 cases only through the portal vein. The values of the blood flow were between 0.4 and 1.0ml pro g liver tissue and minute in portal pressure values about 15 cm water column. Two recirculations were carried out with additional flow through the hepatic artery with about 1/5 of the whole blood flow. The results of the experiments described prove a pretty good functional performance of the perfused with human blood livers of dwarf pigs during several hours. The different animal livers, however, showed at the beginning as well as during the further course of the perfusion clear differences concerning their functional capacity. Decrease of the blood flow values below 0.5 ml pro g liver tissue lead to functional loss. The additional flow through the hepatic artery yielded no better results, compared with the only perfusion of the animal liver through the portal vein.", "contents": "[Studies on the functional ability of swine liver perfused with human blood in machine recirculation attempt. Experiment design, elimination of indocyanine green, galactose, bilirubin and ammonia, synthesis of urea, bile secretion]. In 11 machine experiments of recirculation the abilities of survival and functioning of livers of dwarf pigs perfused with human preserved blood were tested with the help of the eliminations of indocyanine green, galactose, bilirubin and ammonia from a closed circulation. Further urea-nitrogen were measured as indicator of the urea synthesis and bile secretion. The perfusion of the animal livers was carried out in 9 cases only through the portal vein. The values of the blood flow were between 0.4 and 1.0ml pro g liver tissue and minute in portal pressure values about 15 cm water column. Two recirculations were carried out with additional flow through the hepatic artery with about 1/5 of the whole blood flow. The results of the experiments described prove a pretty good functional performance of the perfused with human blood livers of dwarf pigs during several hours. The different animal livers, however, showed at the beginning as well as during the further course of the perfusion clear differences concerning their functional capacity. Decrease of the blood flow values below 0.5 ml pro g liver tissue lead to functional loss. The additional flow through the hepatic artery yielded no better results, compared with the only perfusion of the animal liver through the portal vein.", "PMID": 960861}
{"id": "pubmed23n0032_4067", "title": "[Studies on the functional ability of swine liver perfused with human blood in machine recirculation attempt. 2. Behavior of serochemical parameters].", "content": "In the present paper is reported on the behavior of different serochemical parameters in heterologous perfusion of the liver of pigs. The perfusion of the animal livers was carried out with preserved human blood in 11 recirculation experiments (closed machine circulations). In addition to this an empty circulation with human blood without attachment to an animal liver was carried out, whereby otherwise the arrangement of the experiment was the same, in order to take into consideration the effects of the blood traumatisation in the interpretation of the results. The enzymes GOT and LAP proved as sensible indicators of the lesion of the liver cells occurring in the perfusion of the liver of the pigs. The behavior of the mitochondrial GDH as well as of the lysosomal enzymes SP and BETA-GC which scarcely increased during the recirculations in the perfusate blood, however, allows the conclusion that there did not appear a severe lesion of the liver cells (necrobiosis) during several hours of perfusion. A cholestasis did not develop during the perfusions, when one takes as basis the behavior of the enzymes AP, GGTP and AAP indicating cholestasis. Compared with GOT the enzyme GPT showed by far less elevations in the perfusate blood so that with increasing duration of the perfusion the De-Ritis-quotient significantly increased. Increased LDH-activities above all revealed the increasing during perfusion haemolysis and less reliably a lesion of the liver of the pig. The increase of ADA  in the perfusate blood proved as nearly exclusively conditioned by haemolysis. Total protein, albumins, immunoglobulins, cholinesterase and thymol turbidity test remained unchanged in the course of the perfusions.", "contents": "[Studies on the functional ability of swine liver perfused with human blood in machine recirculation attempt. 2. Behavior of serochemical parameters]. In the present paper is reported on the behavior of different serochemical parameters in heterologous perfusion of the liver of pigs. The perfusion of the animal livers was carried out with preserved human blood in 11 recirculation experiments (closed machine circulations). In addition to this an empty circulation with human blood without attachment to an animal liver was carried out, whereby otherwise the arrangement of the experiment was the same, in order to take into consideration the effects of the blood traumatisation in the interpretation of the results. The enzymes GOT and LAP proved as sensible indicators of the lesion of the liver cells occurring in the perfusion of the liver of the pigs. The behavior of the mitochondrial GDH as well as of the lysosomal enzymes SP and BETA-GC which scarcely increased during the recirculations in the perfusate blood, however, allows the conclusion that there did not appear a severe lesion of the liver cells (necrobiosis) during several hours of perfusion. A cholestasis did not develop during the perfusions, when one takes as basis the behavior of the enzymes AP, GGTP and AAP indicating cholestasis. Compared with GOT the enzyme GPT showed by far less elevations in the perfusate blood so that with increasing duration of the perfusion the De-Ritis-quotient significantly increased. Increased LDH-activities above all revealed the increasing during perfusion haemolysis and less reliably a lesion of the liver of the pig. The increase of ADA  in the perfusate blood proved as nearly exclusively conditioned by haemolysis. Total protein, albumins, immunoglobulins, cholinesterase and thymol turbidity test remained unchanged in the course of the perfusions.", "PMID": 960862}
{"id": "pubmed23n0032_4068", "title": "[Billroth II. Surgery of the stomach].", "content": "In different intervals 220 patients were examined after a resection of the stomach with Billroth's second operation. 179 of them spoke about complaints of different degrees of severity. The cases in question were an early dumping syndrome in 83 patients, which, with the exception of 7 patients, could be removed by simple dietetic measures, such as the elimination of incompatible foodstuff. Again and again the symptoms could be evoked by provocation with it. The method described by Henley proved as correcting operation. Apart from a few exception the fitness for work was not restricted by Billroth's second operation. In shift work or severe physical work a change of the working place was striven for. The body-weight which was reduced already before the operation again increased after operation in 50%, but reached the normal only in 20%. Recidivation ulcera and anastomositis of a suture were rare findings. Stump gastritis is no own clinical picture. Also in this case in histologically still normal mucous membrane earlier histochemical changes develop in the sense of a chronic gastritis with a reduction of the adenosin triphosphatase, the acid tissue phosphatase and succinodehydrogenase.", "contents": "[Billroth II. Surgery of the stomach]. In different intervals 220 patients were examined after a resection of the stomach with Billroth's second operation. 179 of them spoke about complaints of different degrees of severity. The cases in question were an early dumping syndrome in 83 patients, which, with the exception of 7 patients, could be removed by simple dietetic measures, such as the elimination of incompatible foodstuff. Again and again the symptoms could be evoked by provocation with it. The method described by Henley proved as correcting operation. Apart from a few exception the fitness for work was not restricted by Billroth's second operation. In shift work or severe physical work a change of the working place was striven for. The body-weight which was reduced already before the operation again increased after operation in 50%, but reached the normal only in 20%. Recidivation ulcera and anastomositis of a suture were rare findings. Stump gastritis is no own clinical picture. Also in this case in histologically still normal mucous membrane earlier histochemical changes develop in the sense of a chronic gastritis with a reduction of the adenosin triphosphatase, the acid tissue phosphatase and succinodehydrogenase.", "PMID": 960863}
{"id": "pubmed23n0032_4069", "title": "[Comparative examinations of stomach and duodenum with the use of Buscolysin and Cerucal].", "content": "The administration of buscolysin and cerucal in the pharmacoradiography of the stomach and the region of the small intestine was tested on every 50 patients. In these cases the differentiated range of action of the two preparations was shown. Buscolysin can be regarded as remedy of choice for the hypotonic duodenography, whereas cerucal is conspicuously suited for the functional diagnostics especially of the regions of antrum and bulb. By the aimed administration of the two preparations a complex functional diagnostics of stomach and duodenum is possible.", "contents": "[Comparative examinations of stomach and duodenum with the use of Buscolysin and Cerucal]. The administration of buscolysin and cerucal in the pharmacoradiography of the stomach and the region of the small intestine was tested on every 50 patients. In these cases the differentiated range of action of the two preparations was shown. Buscolysin can be regarded as remedy of choice for the hypotonic duodenography, whereas cerucal is conspicuously suited for the functional diagnostics especially of the regions of antrum and bulb. By the aimed administration of the two preparations a complex functional diagnostics of stomach and duodenum is possible.", "PMID": 960864}
{"id": "pubmed23n0032_4070", "title": "[Results of fibrogastroscopic and gastrobioptic examinations after acute acid burn].", "content": "The fibrogastroscopic and gastrobioptic examination of 101 patients after acute intoxication with corrosive substances (vinegar essence, sulphuric acid, hydrochloric acid, lyes a. o.) revealed in all cases a chemical erosion of the gastric mucous membrane of different degree, also in such a case, where there existed neither clinical nor radiological symptoms of the corrosive gastritis. Endoscopically the erosions of the mucous membrane were classified into three degrees of severity: Insignificant erosions were characterized by reddening and swelling of the mucous membrane, foamy phlegm and fluid, medium erosions by numerous puncti-form erosions on the hyperaemic oedematous mucous membrane covered with fibrin, severe erosions, finally, by haemorrhages and dark-gray necrotic areas. Pathohistologically in easy erosions were found a serous or catarrhalic-serous inflammation on the 1st to 5th day, a transition of proliferative processes in regenerative ones on the 6st to 10th day and a complete reparative regeneration on the 11th to 20th day. In medium erosion there existed a catarrhalic or catarrhalic-fibrinous inflammation with erosions on the 1st to 10th day; the regeneration of the mucous membrane took place on the 21st to 30th day. Severe erosions were characterized by a phlegmonous-ulcerous or ulcerous-necrotic inflammation between the 16th and 30th day and the reparative-fibrotic transformation of the mucous membrane between the 31st to the 60th day. After severe erosion no restitution ad integrim took place. There developed either a chronic atrophic gastritis or, when an excessive cicatrisation is present, a stenosis of the prepylorus. A control of the patients including endoscopic examinations which are performed to 6 months after easy erosion, up to one year after medium erosion and up to 5 years after severe erosion, is of importance for the estimation of course, therapy and prognosis.", "contents": "[Results of fibrogastroscopic and gastrobioptic examinations after acute acid burn]. The fibrogastroscopic and gastrobioptic examination of 101 patients after acute intoxication with corrosive substances (vinegar essence, sulphuric acid, hydrochloric acid, lyes a. o.) revealed in all cases a chemical erosion of the gastric mucous membrane of different degree, also in such a case, where there existed neither clinical nor radiological symptoms of the corrosive gastritis. Endoscopically the erosions of the mucous membrane were classified into three degrees of severity: Insignificant erosions were characterized by reddening and swelling of the mucous membrane, foamy phlegm and fluid, medium erosions by numerous puncti-form erosions on the hyperaemic oedematous mucous membrane covered with fibrin, severe erosions, finally, by haemorrhages and dark-gray necrotic areas. Pathohistologically in easy erosions were found a serous or catarrhalic-serous inflammation on the 1st to 5th day, a transition of proliferative processes in regenerative ones on the 6st to 10th day and a complete reparative regeneration on the 11th to 20th day. In medium erosion there existed a catarrhalic or catarrhalic-fibrinous inflammation with erosions on the 1st to 10th day; the regeneration of the mucous membrane took place on the 21st to 30th day. Severe erosions were characterized by a phlegmonous-ulcerous or ulcerous-necrotic inflammation between the 16th and 30th day and the reparative-fibrotic transformation of the mucous membrane between the 31st to the 60th day. After severe erosion no restitution ad integrim took place. There developed either a chronic atrophic gastritis or, when an excessive cicatrisation is present, a stenosis of the prepylorus. A control of the patients including endoscopic examinations which are performed to 6 months after easy erosion, up to one year after medium erosion and up to 5 years after severe erosion, is of importance for the estimation of course, therapy and prognosis.", "PMID": 960865}
{"id": "pubmed23n0032_4071", "title": "[Mathematical statistical evaluation of the trends of biological parameters during intermittent therapy].", "content": "Principle and method of the mathematico-statistical evaluation of an intermitting therapy are explained at the example of the cholestyramine treatment in incomplete obstruction of the bile ducts. The behavior of a biochemical parameter-e.g. the alkaline phosphatase activity and the cholesterol content of the serum-during this time is described with a total approximation function which is subdivided into several partial regression functions. The changes of the biochemical parameter during several periods with and without therapy, respectively, my be characterised and statistically judged from different points of view, e.g. as trend. Thus the clinical test of medicaments gains more objectivity, selectivity, dynamics and individuality. Thus in the example described the effect of cholestyramine (cuemid) decreasing the pathologically increased alkaline phosphatase activity and the increased cholesterol content of the serum and a return to the initial values and a slight deterioration, respectively, after discontinuation of the medicament with a probability of error up to alpha less than 0.001 on one patient could be proved.", "contents": "[Mathematical statistical evaluation of the trends of biological parameters during intermittent therapy]. Principle and method of the mathematico-statistical evaluation of an intermitting therapy are explained at the example of the cholestyramine treatment in incomplete obstruction of the bile ducts. The behavior of a biochemical parameter-e.g. the alkaline phosphatase activity and the cholesterol content of the serum-during this time is described with a total approximation function which is subdivided into several partial regression functions. The changes of the biochemical parameter during several periods with and without therapy, respectively, my be characterised and statistically judged from different points of view, e.g. as trend. Thus the clinical test of medicaments gains more objectivity, selectivity, dynamics and individuality. Thus in the example described the effect of cholestyramine (cuemid) decreasing the pathologically increased alkaline phosphatase activity and the increased cholesterol content of the serum and a return to the initial values and a slight deterioration, respectively, after discontinuation of the medicament with a probability of error up to alpha less than 0.001 on one patient could be proved.", "PMID": 960866}
{"id": "pubmed23n0032_4072", "title": "[X-ray presentation of small intestine invagination during fibroma by means of trochoscopy].", "content": "The roentgenologic diagnosis of tumours of the small intestine often depends on accidental findings. In the case of a 50-year old female patient the indication to operation could be made by the demonstration of the complication (invagination). For the roentgenologic research for tumours in the area of the small intestine, when the result is negative, apart from the oral filling in every case the trochoscopy with transition of the contrast medium into the terminal ileum must be demanded.", "contents": "[X-ray presentation of small intestine invagination during fibroma by means of trochoscopy]. The roentgenologic diagnosis of tumours of the small intestine often depends on accidental findings. In the case of a 50-year old female patient the indication to operation could be made by the demonstration of the complication (invagination). For the roentgenologic research for tumours in the area of the small intestine, when the result is negative, apart from the oral filling in every case the trochoscopy with transition of the contrast medium into the terminal ileum must be demanded.", "PMID": 960867}
{"id": "pubmed23n0032_4073", "title": "[Initial changes in the vascular metabolism and their relations to atherogenesis].", "content": "In a survey initial changes of the metabolism of the vascular wall under the influence of atherogenic noxae in the animal experiment are described. Already a short time after the effect of atherogenic noxae (so-called proliferative phase) the activities of respiratory mitochondrial enzymes in the cells of the smooth muscles of the tunica media of the aorta are considerably reduced in different species. It is a clue to the fact that a hypoxically induced defect in the arterial wall is to be taken into considerations as a pathogenetic factor for arteriosclerosis. Up to now there are no exact proofs for the fact that a transition to a high rate of glycolysis would be predisposing for the appearance of arteriosclerotic changes. The influence of a hypoxia on the vascular wall is not only restricted to the cellular energy metabolism, but stimulates also other cellfunctions. Under hypoxic conditions the synthesis of fatty acids in the arterial tissue is increased. Several authors are of the opinion that a continuous increased supply of lipids overchanges the processes of the cellular regulation which are responsible for the metabolisation of the lipids. The transition into the proliferative phase is characterized by increased insertion rates or transformation rates for different substances in the activated myointimal cells which focally or generally leads to a proliferation or migration into the intima. The proliferating cells distinguish themselves by high activities of different oxidative enzymes which is to be regarded as a reference to an increased readiness of connections rich in energy, according to the high synthetic capacity of these multifunctional mesenchymal cells.", "contents": "[Initial changes in the vascular metabolism and their relations to atherogenesis]. In a survey initial changes of the metabolism of the vascular wall under the influence of atherogenic noxae in the animal experiment are described. Already a short time after the effect of atherogenic noxae (so-called proliferative phase) the activities of respiratory mitochondrial enzymes in the cells of the smooth muscles of the tunica media of the aorta are considerably reduced in different species. It is a clue to the fact that a hypoxically induced defect in the arterial wall is to be taken into considerations as a pathogenetic factor for arteriosclerosis. Up to now there are no exact proofs for the fact that a transition to a high rate of glycolysis would be predisposing for the appearance of arteriosclerotic changes. The influence of a hypoxia on the vascular wall is not only restricted to the cellular energy metabolism, but stimulates also other cellfunctions. Under hypoxic conditions the synthesis of fatty acids in the arterial tissue is increased. Several authors are of the opinion that a continuous increased supply of lipids overchanges the processes of the cellular regulation which are responsible for the metabolisation of the lipids. The transition into the proliferative phase is characterized by increased insertion rates or transformation rates for different substances in the activated myointimal cells which focally or generally leads to a proliferation or migration into the intima. The proliferating cells distinguish themselves by high activities of different oxidative enzymes which is to be regarded as a reference to an increased readiness of connections rich in energy, according to the high synthetic capacity of these multifunctional mesenchymal cells.", "PMID": 960868}
{"id": "pubmed23n0032_4074", "title": "[Significance of clinical and functional diagnostic findings for the physical rehabilitation ability of the patients with heart infarct].", "content": "In 75 patients with condition after myocardial infarction or chronic ischaemic heart disease, respectively, was tested to what extent from clinical and functional-diagnostic findings prognostic estimations concerning the regaining of a cardiocirculatory functional capacity corresponding to age are possible by a complex rehabilitation programme. A daily ergometer training lasting eight weeks which was individually dosed was in the centre of rehabilitation. It was shown that from the findings of ergometry with permanent ECG-registration the effect of rehabilitation, as a rule, can be predicted.", "contents": "[Significance of clinical and functional diagnostic findings for the physical rehabilitation ability of the patients with heart infarct]. In 75 patients with condition after myocardial infarction or chronic ischaemic heart disease, respectively, was tested to what extent from clinical and functional-diagnostic findings prognostic estimations concerning the regaining of a cardiocirculatory functional capacity corresponding to age are possible by a complex rehabilitation programme. A daily ergometer training lasting eight weeks which was individually dosed was in the centre of rehabilitation. It was shown that from the findings of ergometry with permanent ECG-registration the effect of rehabilitation, as a rule, can be predicted.", "PMID": 960869}
{"id": "pubmed23n0032_4075", "title": "[Clinical studies on kinking of the neck-vessels].", "content": "A survey is given of the clinical course of 273 patients hospitalised at one of the departments for internal diseases of a general hospital because of cerebral lesions of different pattern and origin. By means of angiography carried out on 60 patients kinking of the extracranial arteries was proved in 18 cases. Ten patients were submitted to reconstructive vascular surgery: 7 of them are symptom-free. Signs, symptoms and possibilities of surgical or conservative treatment are discussed.", "contents": "[Clinical studies on kinking of the neck-vessels]. A survey is given of the clinical course of 273 patients hospitalised at one of the departments for internal diseases of a general hospital because of cerebral lesions of different pattern and origin. By means of angiography carried out on 60 patients kinking of the extracranial arteries was proved in 18 cases. Ten patients were submitted to reconstructive vascular surgery: 7 of them are symptom-free. Signs, symptoms and possibilities of surgical or conservative treatment are discussed.", "PMID": 960870}
{"id": "pubmed23n0032_4076", "title": "[Progression of rheumatoid arthritis].", "content": "In order to recognize courses with high and low progression in rheumatoid arthritis deviating from former approach the values of a locomotor function test were used which do not belong to the dispersion region for the adequate classes of duration of a disease. With the help of a material of an electronic data processing study of 1,000 cases with ascertained diagnosis correlations between high progression and, among others, following criteria could be ascertained: male sex, old age, early beginning of the disease, manual trades, unfavourable functional values and very much changed laboratory values including high titres of the rheumatoid factor. In this method there was no relation to the duration of the disease, as it was existing with other methods of the determination of progression which were comparatively included into the investigation.", "contents": "[Progression of rheumatoid arthritis]. In order to recognize courses with high and low progression in rheumatoid arthritis deviating from former approach the values of a locomotor function test were used which do not belong to the dispersion region for the adequate classes of duration of a disease. With the help of a material of an electronic data processing study of 1,000 cases with ascertained diagnosis correlations between high progression and, among others, following criteria could be ascertained: male sex, old age, early beginning of the disease, manual trades, unfavourable functional values and very much changed laboratory values including high titres of the rheumatoid factor. In this method there was no relation to the duration of the disease, as it was existing with other methods of the determination of progression which were comparatively included into the investigation.", "PMID": 960871}
{"id": "pubmed23n0032_4077", "title": "[Nephrotic syndrome after treatment with penicillamine in Wilson's disease].", "content": "In a 33-year-old man with Wilson's disease a nephrotic syndrome appeared as a severe complication under penicillamine therapy. Under strong observation and exact controls of protein in the urine, leucocytes, differential blood picture and thrombocytes after interruption of the medication and retrogression of the side-effects a retarding new stabilisation of penicillamine can take place. Other side-effects of penicillamine observed as well as control measures under a long-term treatment with penicillamine are briefly discussed.", "contents": "[Nephrotic syndrome after treatment with penicillamine in Wilson's disease]. In a 33-year-old man with Wilson's disease a nephrotic syndrome appeared as a severe complication under penicillamine therapy. Under strong observation and exact controls of protein in the urine, leucocytes, differential blood picture and thrombocytes after interruption of the medication and retrogression of the side-effects a retarding new stabilisation of penicillamine can take place. Other side-effects of penicillamine observed as well as control measures under a long-term treatment with penicillamine are briefly discussed.", "PMID": 960872}
{"id": "pubmed23n0032_4078", "title": "[Hemodynamic aspects in the evaluation of heart function using systolic time intervals].", "content": "Not invasively determined systolic time intervals give references to the left-ventricular function. Restrictions result from the fact that the systolic time intervals are differently influenced by haemodynamic factors. The inotropic state, the pre and after load, the stroke volume, the frequency as well as the sympathoadrenal system have an effect on the systolic time intervals. In addition to this come specific changes of the haemodynamics by valvular lesions and by pharmaca effective on heart and circulation. In as far about established systolic time intervals clinically usable data concerning the disturbed left-ventricular pumping function may be received, was reported on. On principle with certain restrictions a usable separation of groups between normal and disturbed cardiac function is possible. The judgment of the individual case is restricted due to considerable disperions of individual values. Also by an improved measuring technique as well as by tolerance tests the evidence could not convincingly be improved. On the other hand, with the help of the index of isometric contraction after Rentsch (preejection period in the erect position divided by preejection period in lying position) a separation into normal and beginning left ventricular dysfunction is possible in a well reproducible way.", "contents": "[Hemodynamic aspects in the evaluation of heart function using systolic time intervals]. Not invasively determined systolic time intervals give references to the left-ventricular function. Restrictions result from the fact that the systolic time intervals are differently influenced by haemodynamic factors. The inotropic state, the pre and after load, the stroke volume, the frequency as well as the sympathoadrenal system have an effect on the systolic time intervals. In addition to this come specific changes of the haemodynamics by valvular lesions and by pharmaca effective on heart and circulation. In as far about established systolic time intervals clinically usable data concerning the disturbed left-ventricular pumping function may be received, was reported on. On principle with certain restrictions a usable separation of groups between normal and disturbed cardiac function is possible. The judgment of the individual case is restricted due to considerable disperions of individual values. Also by an improved measuring technique as well as by tolerance tests the evidence could not convincingly be improved. On the other hand, with the help of the index of isometric contraction after Rentsch (preejection period in the erect position divided by preejection period in lying position) a separation into normal and beginning left ventricular dysfunction is possible in a well reproducible way.", "PMID": 960873}
{"id": "pubmed23n0032_4079", "title": "[Diagnosis of coronary insufficiency using bicycle ergometry].", "content": "The bicycle ergometry has proved as screening test for the diagnostics of the latent coronary insufficiency. Taking into consideration well-known prerequisites, indications, contraindications, the criteria of withdrawal and the ECG criteria in coronary insufficiency own results and experiences from an analysis of 1,000 bicycle ergometries are presented and compared with literary data.", "contents": "[Diagnosis of coronary insufficiency using bicycle ergometry]. The bicycle ergometry has proved as screening test for the diagnostics of the latent coronary insufficiency. Taking into consideration well-known prerequisites, indications, contraindications, the criteria of withdrawal and the ECG criteria in coronary insufficiency own results and experiences from an analysis of 1,000 bicycle ergometries are presented and compared with literary data.", "PMID": 960874}
{"id": "pubmed23n0032_4080", "title": "[Cholegraphy with protracted mechanical infusion as a method of routine].", "content": "250 out-patients were examined by mechanical 30 min slow-infusion cholegraphy with 20 ml jodipamide (Adipiodon). Only 1.2% slight and 2.0% distinct side effects were established. The frequency and severity of these reactions were less compared with the usual intravenous cholecystography. The described method of investigation may be recommended for use.", "contents": "[Cholegraphy with protracted mechanical infusion as a method of routine]. 250 out-patients were examined by mechanical 30 min slow-infusion cholegraphy with 20 ml jodipamide (Adipiodon). Only 1.2% slight and 2.0% distinct side effects were established. The frequency and severity of these reactions were less compared with the usual intravenous cholecystography. The described method of investigation may be recommended for use.", "PMID": 960875}
{"id": "pubmed23n0032_4081", "title": "[Electroencephalographic findings in liver function disorders].", "content": "Abnormal rhythmisations in the EEG are described in hepatoportal encephalopathy as an expression of a disturbance of more profound cerebral structures. The EEG gives an actual information about the state of the cerebral functions of all stages of a hepato-cerebral decompensation and allows a reliable prognostic estimation during the observation of the course. Practically significant are also bioelectric findings of a disturbance of the cerebral function with clinically symptom-poor course in compensated liver cirrhosis or obstructive icterus.", "contents": "[Electroencephalographic findings in liver function disorders]. Abnormal rhythmisations in the EEG are described in hepatoportal encephalopathy as an expression of a disturbance of more profound cerebral structures. The EEG gives an actual information about the state of the cerebral functions of all stages of a hepato-cerebral decompensation and allows a reliable prognostic estimation during the observation of the course. Practically significant are also bioelectric findings of a disturbance of the cerebral function with clinically symptom-poor course in compensated liver cirrhosis or obstructive icterus.", "PMID": 960876}
{"id": "pubmed23n0032_4082", "title": "[Humoral immune reaction in chronic liver diseases].", "content": "In 60 patients with a morphologically ascertained chronic liver disease and 40 hepatologically examined patients with a healthy liver of a control group the quantitative determination of the immunoglobulin and the immunofluorescence-serological determination of antibodies against nuclei, smooth musculature and mitochondria were carried out. Only in one female patient with a chronic active hepatitis out of 51 patients with morphologically ascertained liver cirrhosis or chronic active hepatitis antibodies against nuclei and smooth musculature in a level of the titre of more than 1 : 40 and only in 2 female patients with a primary biliary cirrhosis antibodies against mitochondria could be proved in a level of the titre of more than 160. Titres of antibodies lying below were found in the group of patients with liver diseases and the control group in the same frequency, so that an autoimmune form of the cryptogenic cirrhosis could not be differentiated. The proof of antibodies against nuclei and smooth musculature of a high titre in connection with an isolated increase of the IgG is of special diagnostic importance for the autoimmune form of the chronic active hepatitis; the same is the case in the proof of antibodies against mitochondria of a high titre in connection with an isolated increase of IgM.", "contents": "[Humoral immune reaction in chronic liver diseases]. In 60 patients with a morphologically ascertained chronic liver disease and 40 hepatologically examined patients with a healthy liver of a control group the quantitative determination of the immunoglobulin and the immunofluorescence-serological determination of antibodies against nuclei, smooth musculature and mitochondria were carried out. Only in one female patient with a chronic active hepatitis out of 51 patients with morphologically ascertained liver cirrhosis or chronic active hepatitis antibodies against nuclei and smooth musculature in a level of the titre of more than 1 : 40 and only in 2 female patients with a primary biliary cirrhosis antibodies against mitochondria could be proved in a level of the titre of more than 160. Titres of antibodies lying below were found in the group of patients with liver diseases and the control group in the same frequency, so that an autoimmune form of the cryptogenic cirrhosis could not be differentiated. The proof of antibodies against nuclei and smooth musculature of a high titre in connection with an isolated increase of the IgG is of special diagnostic importance for the autoimmune form of the chronic active hepatitis; the same is the case in the proof of antibodies against mitochondria of a high titre in connection with an isolated increase of IgM.", "PMID": 960877}
{"id": "pubmed23n0032_4083", "title": "[Indications, adverse effects and complications of lithium therapy].", "content": "The application of lithium preparations in the therapy and prophylaxis of recidivations of affective psychoses has essentially gathered in point in psychiatry during the last years. Apart from the discussion of the indication, the contraindication and efficacy especially concomitant appearances, side effects and dangers as well as their influencibility are demonstrated, the knowledge of which is of importance also for the physician not dealing with psychiatry.", "contents": "[Indications, adverse effects and complications of lithium therapy]. The application of lithium preparations in the therapy and prophylaxis of recidivations of affective psychoses has essentially gathered in point in psychiatry during the last years. Apart from the discussion of the indication, the contraindication and efficacy especially concomitant appearances, side effects and dangers as well as their influencibility are demonstrated, the knowledge of which is of importance also for the physician not dealing with psychiatry.", "PMID": 960878}
{"id": "pubmed23n0032_4084", "title": "[Objectivization of the protamine sulfate test through photometric measurements].", "content": "The demonstration of monomeric complexes of fibrin in the blood is regarded as a secure sign of an increased consumption of coagulation factors in the sense of a consumption coagulopathy. As a simple and sensitive method for the demonstration of these intermediate products of the coagulation the protamine sulphate test proved its worth in clinical practice. The subjective judgment of this test-tube test frequently becomes difficult in the borderline region between normal and pathologic. On the basis of own comparative investigations it could, however, be demonstrated that by means of photometric measurements an objective and exact evidence about the result of the protamine sulphate test can be made. It is referred to the advantages in the judgment of the course of a consumption coagulopathy during the treatment.", "contents": "[Objectivization of the protamine sulfate test through photometric measurements]. The demonstration of monomeric complexes of fibrin in the blood is regarded as a secure sign of an increased consumption of coagulation factors in the sense of a consumption coagulopathy. As a simple and sensitive method for the demonstration of these intermediate products of the coagulation the protamine sulphate test proved its worth in clinical practice. The subjective judgment of this test-tube test frequently becomes difficult in the borderline region between normal and pathologic. On the basis of own comparative investigations it could, however, be demonstrated that by means of photometric measurements an objective and exact evidence about the result of the protamine sulphate test can be made. It is referred to the advantages in the judgment of the course of a consumption coagulopathy during the treatment.", "PMID": 960879}
{"id": "pubmed23n0032_4085", "title": "[Diagnosis and therapy of Crohn's disease].", "content": "Crohn's ileocolitis is an etiologically unclarified inflammatory disease of the alimentary canal, which is in most cases arranged in segments, may concern all segments, but above all may take place in the inferior ileum and the adjacent segments of the colon. The diagnosis which formerly was certainly made too infrequently is difficult, but histologically it is to be ascertained by typical granulomas of the epitheloid cells. The disease which extraordinarily often is connected with recidivations demands a consequent conservative combination therapy with salazopyrine and cortisone, in acute exacerbations and in formations of fistulae an additional prescription of bactericidal broad spectrum antibiotics is necessary. Resistent courses justify the application of immunosuppressive drugs. The indication to operation is critically to be given and to be restricted to the resection of stenosing narrowings of the lumen.", "contents": "[Diagnosis and therapy of Crohn's disease]. Crohn's ileocolitis is an etiologically unclarified inflammatory disease of the alimentary canal, which is in most cases arranged in segments, may concern all segments, but above all may take place in the inferior ileum and the adjacent segments of the colon. The diagnosis which formerly was certainly made too infrequently is difficult, but histologically it is to be ascertained by typical granulomas of the epitheloid cells. The disease which extraordinarily often is connected with recidivations demands a consequent conservative combination therapy with salazopyrine and cortisone, in acute exacerbations and in formations of fistulae an additional prescription of bactericidal broad spectrum antibiotics is necessary. Resistent courses justify the application of immunosuppressive drugs. The indication to operation is critically to be given and to be restricted to the resection of stenosing narrowings of the lumen.", "PMID": 960880}
{"id": "pubmed23n0032_4086", "title": "[Studies on the diaplacental transmission of Masugi-nephritis in the rabbit].", "content": "A diaplacentar transmission of the Masugi nephritis or the evocation of a clinical picture similar to the congenital glomerulonephritis could not be achieved in rabbits. Also, the juxtaglomerular index-values of the experimental group did not show a significant difference referred to the control group.", "contents": "[Studies on the diaplacental transmission of Masugi-nephritis in the rabbit]. A diaplacentar transmission of the Masugi nephritis or the evocation of a clinical picture similar to the congenital glomerulonephritis could not be achieved in rabbits. Also, the juxtaglomerular index-values of the experimental group did not show a significant difference referred to the control group.", "PMID": 960881}
{"id": "pubmed23n0032_4087", "title": "[Alanine aminopeptidase and creatinine excretion under various conditions of diuresis in man].", "content": "In 10 female test persons Volhard's water and concentration experiment was carried out. It exists a highly significant correlation between the alanine aminopeptidase activity and the creatinine concentration in the urine. The relation of the two factors to the urine minute volume is hyperbolic. The creatinine in the urine may be regarded as a favourable reference parameter for the excretion of alanine aminopeptidase.", "contents": "[Alanine aminopeptidase and creatinine excretion under various conditions of diuresis in man]. In 10 female test persons Volhard's water and concentration experiment was carried out. It exists a highly significant correlation between the alanine aminopeptidase activity and the creatinine concentration in the urine. The relation of the two factors to the urine minute volume is hyperbolic. The creatinine in the urine may be regarded as a favourable reference parameter for the excretion of alanine aminopeptidase.", "PMID": 960882}
{"id": "pubmed23n0032_4088", "title": "[Post-traumatic meningitis due to Pasteurella multocida].", "content": "The clinical data of a 14-year old female patient with posttraumatic meningitis by Pasteurella multocida are reported. The behaviour of the isolated strain is recorded in cultural and biochemical aspect as well as in animal experiment. The importance of the infection with Pasteurella multocida is lined out and the possible origin of the disease in the reported case is discussed. Finally some problems of special bacteriological diagnosis are explained and hints are given in this respect.", "contents": "[Post-traumatic meningitis due to Pasteurella multocida]. The clinical data of a 14-year old female patient with posttraumatic meningitis by Pasteurella multocida are reported. The behaviour of the isolated strain is recorded in cultural and biochemical aspect as well as in animal experiment. The importance of the infection with Pasteurella multocida is lined out and the possible origin of the disease in the reported case is discussed. Finally some problems of special bacteriological diagnosis are explained and hints are given in this respect.", "PMID": 960883}
{"id": "pubmed23n0032_4089", "title": "[Value and worthlessness of clinical studies in the diagnosis of brain death].", "content": "Death was ever determined on the basis of extinguished partial functions of the entire organism (partial death). In intensive medicine with its possibilities of reanimation, with its possibilities of artificial maintenance of breathing and circulation the individual death of man is equated with his cerebral death. It comes only under conditions of reanimation and nearly always shows a clinically recognizable development. Practically above all \"the syndromes in the forefield of cerebral death\" are of interest as well as its obligatory and optional symptoms, the valency of which was critically tested. A 5-year-analysis (1969 to 1973) of 487 deaths in an internal intensive therapy unit (18% of mortality) was shown that causes, frequency and age distribution of the mortal conditions of disease as well as the average survival time of 4.5 days, in which cases, however, 43% of all deaths occurred within the first 24 hours, very rarely caused us to establish the irreversibility of the loss of the cerebral function on account of an  organic dysfunction, but rather resulting from the question of the interruption of an absurd reanimation.", "contents": "[Value and worthlessness of clinical studies in the diagnosis of brain death]. Death was ever determined on the basis of extinguished partial functions of the entire organism (partial death). In intensive medicine with its possibilities of reanimation, with its possibilities of artificial maintenance of breathing and circulation the individual death of man is equated with his cerebral death. It comes only under conditions of reanimation and nearly always shows a clinically recognizable development. Practically above all \"the syndromes in the forefield of cerebral death\" are of interest as well as its obligatory and optional symptoms, the valency of which was critically tested. A 5-year-analysis (1969 to 1973) of 487 deaths in an internal intensive therapy unit (18% of mortality) was shown that causes, frequency and age distribution of the mortal conditions of disease as well as the average survival time of 4.5 days, in which cases, however, 43% of all deaths occurred within the first 24 hours, very rarely caused us to establish the irreversibility of the loss of the cerebral function on account of an  organic dysfunction, but rather resulting from the question of the interruption of an absurd reanimation.", "PMID": 960884}
{"id": "pubmed23n0032_4090", "title": "[Cardiopulmonary diagnosis in patients with ischemic heart disease].", "content": "It may be established that the complex cardiopulmonary functional diagnostics in patients with chronic ischaemic heart disease obtained the following essential results: 1. The ergometrically achieved total functional capacity is clearly decreased in all age groups compared with the healthy persons, the differences are highly significant. 2. The proof of a coronary insufficiency got by the electrocardiogram after work is to be regarded as a factor limiting the functional capacity. 3. 72% of the patients reveal under load a PAEDP increased more than the normal of 25 Torr. After exclusion of a respiratory insufficiency these findings must be regarded as a disturbed myocardial function. 4. Thus the increased PAEDP under load apart from the well-known triad (angina pectoris under load, decreased total functional capacity, pathological ECG after work) is a sensitive and decisive factor for proving the disturbed cardial function in chronic ischaemic heart disease.", "contents": "[Cardiopulmonary diagnosis in patients with ischemic heart disease]. It may be established that the complex cardiopulmonary functional diagnostics in patients with chronic ischaemic heart disease obtained the following essential results: 1. The ergometrically achieved total functional capacity is clearly decreased in all age groups compared with the healthy persons, the differences are highly significant. 2. The proof of a coronary insufficiency got by the electrocardiogram after work is to be regarded as a factor limiting the functional capacity. 3. 72% of the patients reveal under load a PAEDP increased more than the normal of 25 Torr. After exclusion of a respiratory insufficiency these findings must be regarded as a disturbed myocardial function. 4. Thus the increased PAEDP under load apart from the well-known triad (angina pectoris under load, decreased total functional capacity, pathological ECG after work) is a sensitive and decisive factor for proving the disturbed cardial function in chronic ischaemic heart disease.", "PMID": 960885}
{"id": "pubmed23n0032_4091", "title": "[The value of the diastolic pulmonary arterial pressure for the estimation of the cardiac functional capacity in patients with myocardial infarct].", "content": "From 4 weeks to several months after infarction the patients revealed an altogether clearly decreased total physical functional capacity compared with healthy persons. Behaviour of pulse and blood pressure as well as ventilatory indices in comparable Watt-degrees did not reveal any significant differences compared with persons with a healthy heart. The stroke volume pro surface and the heart-time-volume did also not reveal any determinable deviation concerning the mean values compared with normal persons. In patients with compensated infarction the pulmonary arterial pressure in rest was, as a rule, within the normal. However, under load it increased more than in healthy persons. Patients with decompensated infarction revealed a PAEDP in rest between 20 and more than 30 Torr. The question about a critical PAEDP under load should further be pursued. The ECG gives reliable limiting findings in functional examinations.", "contents": "[The value of the diastolic pulmonary arterial pressure for the estimation of the cardiac functional capacity in patients with myocardial infarct]. From 4 weeks to several months after infarction the patients revealed an altogether clearly decreased total physical functional capacity compared with healthy persons. Behaviour of pulse and blood pressure as well as ventilatory indices in comparable Watt-degrees did not reveal any significant differences compared with persons with a healthy heart. The stroke volume pro surface and the heart-time-volume did also not reveal any determinable deviation concerning the mean values compared with normal persons. In patients with compensated infarction the pulmonary arterial pressure in rest was, as a rule, within the normal. However, under load it increased more than in healthy persons. Patients with decompensated infarction revealed a PAEDP in rest between 20 and more than 30 Torr. The question about a critical PAEDP under load should further be pursued. The ECG gives reliable limiting findings in functional examinations.", "PMID": 960886}
{"id": "pubmed23n0032_4092", "title": "[The effect of an ambulatory long term training on performance, metabolic and coagulation parameters after myocardial infarct].", "content": "It is reported on the results of a training treatment for outpatients after myocardial infarction which was carried out twice a week during one and a half year, compared to a control group without physical conditioning. Out of 30 patients 14 continuously took part in the training lessons. In contrast to the control group in the training group a clear increase of the working capacity, a slight decrease of the body-weight, of the blood pressure in rest and of the triglycerides in the serum as well as a favourable influence on the glucose tolerance could be proved. The serum cholesterol level and the coagulation parameters did not change significantly. One patient of the training group and one of the control group died. One patient of the training group and one patient of the control group suffered a reinfarction.", "contents": "[The effect of an ambulatory long term training on performance, metabolic and coagulation parameters after myocardial infarct]. It is reported on the results of a training treatment for outpatients after myocardial infarction which was carried out twice a week during one and a half year, compared to a control group without physical conditioning. Out of 30 patients 14 continuously took part in the training lessons. In contrast to the control group in the training group a clear increase of the working capacity, a slight decrease of the body-weight, of the blood pressure in rest and of the triglycerides in the serum as well as a favourable influence on the glucose tolerance could be proved. The serum cholesterol level and the coagulation parameters did not change significantly. One patient of the training group and one of the control group died. One patient of the training group and one patient of the control group suffered a reinfarction.", "PMID": 960887}
{"id": "pubmed23n0032_4093", "title": "[The objective gradation of stenosing coronary artery processes by means of the quantification of pathologic-anatomical data and their relations. 2. Development and presentation of the model].", "content": "In order to objectify the judgement of the coronary arteriosclerosis the influence values position x obstruction on the left side and on the right side as well as the mass of the heart are mathematically valued with the aim to achieve a good separation between the infarction group and the control group and an insignificant false coordination of test persons. The optimation carried out for this purpose must be brought into accordance by means of technically ascertained recognitions and necessary practicability concerning valuations which are to be proposed. According to the results obtained on 174 test persons an obstruction on the right side below 50% has a higher importance than on the left side. In the positions of the obstruction the proximal as well as the medium third of the right coronary arteries a higher valuation must be ascribed. Compared with this the trunk of the left coronary artery has a significance corresponding possibly to the proximal third of this vessel. A more insignificant importance than hitherto taken into consideration belongs to the medium third of the left coronary artery. The valuations proposed lead to a highly significant improvement of the separation ability in contrast to the comparable valuations. The false coordination of the test persons at the same time decreases to 11 in the group with infarctions and to 2 in the control group. This equals 10.1 or 4.5%, respectively. In the present number of patients the barriers of the ostium and the type of supply have no statistically ascertained influence on the separation ability.", "contents": "[The objective gradation of stenosing coronary artery processes by means of the quantification of pathologic-anatomical data and their relations. 2. Development and presentation of the model]. In order to objectify the judgement of the coronary arteriosclerosis the influence values position x obstruction on the left side and on the right side as well as the mass of the heart are mathematically valued with the aim to achieve a good separation between the infarction group and the control group and an insignificant false coordination of test persons. The optimation carried out for this purpose must be brought into accordance by means of technically ascertained recognitions and necessary practicability concerning valuations which are to be proposed. According to the results obtained on 174 test persons an obstruction on the right side below 50% has a higher importance than on the left side. In the positions of the obstruction the proximal as well as the medium third of the right coronary arteries a higher valuation must be ascribed. Compared with this the trunk of the left coronary artery has a significance corresponding possibly to the proximal third of this vessel. A more insignificant importance than hitherto taken into consideration belongs to the medium third of the left coronary artery. The valuations proposed lead to a highly significant improvement of the separation ability in contrast to the comparable valuations. The false coordination of the test persons at the same time decreases to 11 in the group with infarctions and to 2 in the control group. This equals 10.1 or 4.5%, respectively. In the present number of patients the barriers of the ostium and the type of supply have no statistically ascertained influence on the separation ability.", "PMID": 960888}
{"id": "pubmed23n0032_4094", "title": "[Studies on the specificity of alanine aminopeptidase and its variants in the serum in hepatobiliary diseases].", "content": "After examinations on a mixed number of patients (236 patients) the increases of the activities of the alanine aminopeptidase in the serum with relatively high certainty may be traced back to diseases of the liver and of the bile ducts. The hepatobiliary specifity is disturbed only by generalised dermatological diseases, especially eczemas. Changes in the electrophoretic pattern of the serum alanine aminopeptidase are also relatively specific for diseases or lesions of the hepatobiliary system. In 11 of the 12 anomalous zymograms of the alanine aminopeptidase observed among 236 patients the aimed search resulted in an alteration of the hepatobiliary system.", "contents": "[Studies on the specificity of alanine aminopeptidase and its variants in the serum in hepatobiliary diseases]. After examinations on a mixed number of patients (236 patients) the increases of the activities of the alanine aminopeptidase in the serum with relatively high certainty may be traced back to diseases of the liver and of the bile ducts. The hepatobiliary specifity is disturbed only by generalised dermatological diseases, especially eczemas. Changes in the electrophoretic pattern of the serum alanine aminopeptidase are also relatively specific for diseases or lesions of the hepatobiliary system. In 11 of the 12 anomalous zymograms of the alanine aminopeptidase observed among 236 patients the aimed search resulted in an alteration of the hepatobiliary system.", "PMID": 960889}
{"id": "pubmed23n0032_4095", "title": "[Comparative intravenous and cholangiographic infusion studies in chronic diseases of the bile ducts].", "content": "Compared with the intravenous cholangiography the infusion cholangiography is the more effective and unambiguous investigation method of the gallbladder and the bile ducts. It considerably enlarges the diagnostic possibilities in the area of the bile ducts. A serum bilirubin level of more than 2 mg/100 ml allows positive cholangiograms by means of the infusion method, but is nearly always the cause for negative findings in intravenous application. In a hypalbuminaemia lower than 3 g/100 ml, in an increase of the alkaline serum phosphatase, of the serum transaminase and a pathological retention of bromsulphalein the bile ducts cannot be demonstrated also in the infusion cholangiography. A 20% glucose solution is sufficient as solvent in the infusion cholangiography. In the negative case the cholecystography and the intravenous cholangiography may be supplemented and enlarged by application of the infusion cholangiography as a more evident method of X-ray examination.", "contents": "[Comparative intravenous and cholangiographic infusion studies in chronic diseases of the bile ducts]. Compared with the intravenous cholangiography the infusion cholangiography is the more effective and unambiguous investigation method of the gallbladder and the bile ducts. It considerably enlarges the diagnostic possibilities in the area of the bile ducts. A serum bilirubin level of more than 2 mg/100 ml allows positive cholangiograms by means of the infusion method, but is nearly always the cause for negative findings in intravenous application. In a hypalbuminaemia lower than 3 g/100 ml, in an increase of the alkaline serum phosphatase, of the serum transaminase and a pathological retention of bromsulphalein the bile ducts cannot be demonstrated also in the infusion cholangiography. A 20% glucose solution is sufficient as solvent in the infusion cholangiography. In the negative case the cholecystography and the intravenous cholangiography may be supplemented and enlarged by application of the infusion cholangiography as a more evident method of X-ray examination.", "PMID": 960890}
{"id": "pubmed23n0032_4096", "title": "[Physiotherapeutic viewpoints in progressive scleroderma].", "content": "The hitherto given recommendations for physiotherapy in progressive sclerodermia are in most cases undifferentiated. Proceeding from the aims of an influence of sclerodermic changes of the tissues, a prevention of restrictions of the movement and an improvement of the general condition a classification of suitable physiotherapeutic measures is recommended.", "contents": "[Physiotherapeutic viewpoints in progressive scleroderma]. The hitherto given recommendations for physiotherapy in progressive sclerodermia are in most cases undifferentiated. Proceeding from the aims of an influence of sclerodermic changes of the tissues, a prevention of restrictions of the movement and an improvement of the general condition a classification of suitable physiotherapeutic measures is recommended.", "PMID": 960891}
{"id": "pubmed23n0032_4097", "title": "[Long-range therapy of hypertension].", "content": "In 572 patients with hypertension a long-term therapy with alpha-methyldopa (Dopegyt) was carried out and this preparation was found to be highly effective. The preparation decreases the blood pressure in standing position as well as in lying position reducing mainly the peripheral vascular resistance. It is well absorbed orally. As a rule, the minute output of the heart is maintained without acceleration of the heart rate. An orthostatic hypotension was only rarely to be observed and only in a very small degree. With the help of the isotope renography could be proved that the treatment with alpha-methyldopa causes no reduction of the blood supply of the kidneys, wherefore alpha-methyldopa can be successfully applied in patients with hypertension and renal failure. Side effects appeared very rarely. During the application of 34,550 tablets of alpha-methyldopa only once a haemolytic anaemia was observed. In patients with a severe hypertension the combination alpha-methyldopa (Dopegyt) + etacrynic acid (Uregyt) evoked a distinct decrease of the systolic and diastolic blood pressure.", "contents": "[Long-range therapy of hypertension]. In 572 patients with hypertension a long-term therapy with alpha-methyldopa (Dopegyt) was carried out and this preparation was found to be highly effective. The preparation decreases the blood pressure in standing position as well as in lying position reducing mainly the peripheral vascular resistance. It is well absorbed orally. As a rule, the minute output of the heart is maintained without acceleration of the heart rate. An orthostatic hypotension was only rarely to be observed and only in a very small degree. With the help of the isotope renography could be proved that the treatment with alpha-methyldopa causes no reduction of the blood supply of the kidneys, wherefore alpha-methyldopa can be successfully applied in patients with hypertension and renal failure. Side effects appeared very rarely. During the application of 34,550 tablets of alpha-methyldopa only once a haemolytic anaemia was observed. In patients with a severe hypertension the combination alpha-methyldopa (Dopegyt) + etacrynic acid (Uregyt) evoked a distinct decrease of the systolic and diastolic blood pressure.", "PMID": 960892}
{"id": "pubmed23n0032_4098", "title": "[Therapy of chronic ischemic heart disease and arterial hypertension by means of beta-receptor blockaders].", "content": "50 patients with stenocardias in persisting arterial hypertension, of them 33 manifest coronary diseases, were orally treated with doses of propranolol up to 400 mg/die during a longer period. In the majority of the cases a good to very good therapeutic effectivity was to be proved in hypertension as well as in chronic ischaemic heart disease. The own favourable results of the treatment justify the estimation that proporanolol may be regarded as therapeutic remedy of choice in patients with simultaneous hypertension and chronic ischaemic heart disease. Moreover, a collective of 45 patients with different cardiovascular diagnoses under the new selective beta-receptor blocker Bonnecor (practolol) was observed. It is reported on first experiences in the sense of an intermediate information.", "contents": "[Therapy of chronic ischemic heart disease and arterial hypertension by means of beta-receptor blockaders]. 50 patients with stenocardias in persisting arterial hypertension, of them 33 manifest coronary diseases, were orally treated with doses of propranolol up to 400 mg/die during a longer period. In the majority of the cases a good to very good therapeutic effectivity was to be proved in hypertension as well as in chronic ischaemic heart disease. The own favourable results of the treatment justify the estimation that proporanolol may be regarded as therapeutic remedy of choice in patients with simultaneous hypertension and chronic ischaemic heart disease. Moreover, a collective of 45 patients with different cardiovascular diagnoses under the new selective beta-receptor blocker Bonnecor (practolol) was observed. It is reported on first experiences in the sense of an intermediate information.", "PMID": 960893}
{"id": "pubmed23n0032_4099", "title": "[The frequency and form of manifestation of liver damage under INH therapy].", "content": "It is reported on 305 clinically examined patients with the diagnosis of a functional colon syndrome. In 65 patients somatic diseases were found to be the possible triggering factors. In contrast to a comparative group in the other 240 patients in the former anamnesis more frequently allergies, appendectomies and chronic application of purgatives were to be established. The most frequent complaints were irregularity of defaecation in form of spastic obstipation (62.9%) or diarrhoea (35.4%), intolerance to foodstuff (100%), pain in the colon(66.7%) and meteorism (65.4%). The 4th decennium was most frequently concerned in men as well as in women.", "contents": "[The frequency and form of manifestation of liver damage under INH therapy]. It is reported on 305 clinically examined patients with the diagnosis of a functional colon syndrome. In 65 patients somatic diseases were found to be the possible triggering factors. In contrast to a comparative group in the other 240 patients in the former anamnesis more frequently allergies, appendectomies and chronic application of purgatives were to be established. The most frequent complaints were irregularity of defaecation in form of spastic obstipation (62.9%) or diarrhoea (35.4%), intolerance to foodstuff (100%), pain in the colon(66.7%) and meteorism (65.4%). The 4th decennium was most frequently concerned in men as well as in women.", "PMID": 960894}
{"id": "pubmed23n0032_4100", "title": "[Diabetes mellitus, hyperuricemia and pyelonephritis].", "content": "The following groups of patients are discussed. 1st group: 51 patients with diabetes mellitus and chronic pyelonephritis, 2nd group: 15 patients with diabetes, hyperuricaemia, adiposity and pyelonephritis. It was established that in the first group pyelonephritis was therapeutically well to be influenced by antiobiotics and chemotherapeutics after stopping of diabetes. In the second group the success was less satisfactory and it was also to be achieved retardedly on account of the massive renal lesion, by the combination of diabetes and uricopathy. The clinical syndrome of diabetes mellitus, hyperuricaemia and adiposity is discussed.", "contents": "[Diabetes mellitus, hyperuricemia and pyelonephritis]. The following groups of patients are discussed. 1st group: 51 patients with diabetes mellitus and chronic pyelonephritis, 2nd group: 15 patients with diabetes, hyperuricaemia, adiposity and pyelonephritis. It was established that in the first group pyelonephritis was therapeutically well to be influenced by antiobiotics and chemotherapeutics after stopping of diabetes. In the second group the success was less satisfactory and it was also to be achieved retardedly on account of the massive renal lesion, by the combination of diabetes and uricopathy. The clinical syndrome of diabetes mellitus, hyperuricaemia and adiposity is discussed.", "PMID": 960895}
{"id": "pubmed23n0032_4101", "title": "[Latent diabetes mellitus in families with diabetes and ischemic heart disease].", "content": "In 30 diabetics and their nearest relations (44 persons) the immunoreactive insulin (IRI) was examined and a routine glucose tolerance test was carried out as well as in patients with ischaemic heart disease (more than 100 patients). The control group consisted of 30 healthy volunteers. An analysis of the results was performed taking into consideration the degrees of severity and the duration of the diabetes as well as the age of the patients. A conclusion was drawn that the exhaustion of Langerhans islets occurs relatively early during \"juvenile\" diabetes. A clear decrease of the IRI corresponding to the degree of severity and to the duration of diabetes can be observed. In the nearest relations of the diabetics a normal IRI-level and in a relatively little percentage latent forms of a diabetes were detected. In patients with ischaemic heart disease however, the non-manifest forms of diabetes were by far more frequent. It is considered that more precise tests should be applied in laboratory diagnosis of latent diabetes mellitus in hereditarily aggravated persons in the mentioned respect.", "contents": "[Latent diabetes mellitus in families with diabetes and ischemic heart disease]. In 30 diabetics and their nearest relations (44 persons) the immunoreactive insulin (IRI) was examined and a routine glucose tolerance test was carried out as well as in patients with ischaemic heart disease (more than 100 patients). The control group consisted of 30 healthy volunteers. An analysis of the results was performed taking into consideration the degrees of severity and the duration of the diabetes as well as the age of the patients. A conclusion was drawn that the exhaustion of Langerhans islets occurs relatively early during \"juvenile\" diabetes. A clear decrease of the IRI corresponding to the degree of severity and to the duration of diabetes can be observed. In the nearest relations of the diabetics a normal IRI-level and in a relatively little percentage latent forms of a diabetes were detected. In patients with ischaemic heart disease however, the non-manifest forms of diabetes were by far more frequent. It is considered that more precise tests should be applied in laboratory diagnosis of latent diabetes mellitus in hereditarily aggravated persons in the mentioned respect.", "PMID": 960896}
{"id": "pubmed23n0032_4102", "title": "[Experiences with cytostatic treatment of malignant tumors].", "content": "The methods and the results of the cytostatic permanent and impact therapy, of the partial synchronisation as well as of the cytostatic combination treatment are described, taking into special consideration inoperable or metastasized solid tumours. From the study which was carried out during a period of 15 years results that a combination treatment referred in the individual doses to body weight and blood picture apparantly achieves better results than the other forms of therapy, whereby the risk of complications is relatively low.", "contents": "[Experiences with cytostatic treatment of malignant tumors]. The methods and the results of the cytostatic permanent and impact therapy, of the partial synchronisation as well as of the cytostatic combination treatment are described, taking into special consideration inoperable or metastasized solid tumours. From the study which was carried out during a period of 15 years results that a combination treatment referred in the individual doses to body weight and blood picture apparantly achieves better results than the other forms of therapy, whereby the risk of complications is relatively low.", "PMID": 960897}
{"id": "pubmed23n0032_4103", "title": "[Experiences in short-term dialyses with 2 capillary dialyzers in parallel and serial circuit in 2 and 3 shift operations].", "content": "It is reported on experiences made in 200 short-term dialyses with every 2 capillary dialysators. 167 dialyses of which 112 were performed by means of parallel arrangement and 55 by means of serial arrangement of the dialysators are analysed in detail. In their effectivity of dialysis the two variants proved to be nearly equivalent, in which cases the series-connection is of practical advantage. In two shifts of nurses up to 3 shifts of patients could be treated. In the large area dialysis an adaptation  of the dialysate and a more intensive control of the patients must be performed. Advantages and disadvantages of this method are discussed.", "contents": "[Experiences in short-term dialyses with 2 capillary dialyzers in parallel and serial circuit in 2 and 3 shift operations]. It is reported on experiences made in 200 short-term dialyses with every 2 capillary dialysators. 167 dialyses of which 112 were performed by means of parallel arrangement and 55 by means of serial arrangement of the dialysators are analysed in detail. In their effectivity of dialysis the two variants proved to be nearly equivalent, in which cases the series-connection is of practical advantage. In two shifts of nurses up to 3 shifts of patients could be treated. In the large area dialysis an adaptation  of the dialysate and a more intensive control of the patients must be performed. Advantages and disadvantages of this method are discussed.", "PMID": 960898}
{"id": "pubmed23n0032_4104", "title": "[General aspects of cytostatic therapy of hemoblastoses].", "content": "In haemoblastoses the chemotherapy is able to achieve remissions and prolongations of the survival time. The combined or polychemotherapy, respectively, is here comparatively more effective than the monotherapy. In haemoblastoses the concept of the maximal destruction of tumours (induction therapy) could be realised by means of consolidation and maintenance therapy. The prediction of the therapy effects by inclusion of different host parameters (morphology, immune state, age, sensibility test) may only approximately be done. In the induction and maintenance of the remission the supportive therapeutic measures play an essential role. By the development of selective cytostatic drugs and further utilisation of the results of molecular-biological experiments further ameliorations of the therapeutic situation are to be awaited.", "contents": "[General aspects of cytostatic therapy of hemoblastoses]. In haemoblastoses the chemotherapy is able to achieve remissions and prolongations of the survival time. The combined or polychemotherapy, respectively, is here comparatively more effective than the monotherapy. In haemoblastoses the concept of the maximal destruction of tumours (induction therapy) could be realised by means of consolidation and maintenance therapy. The prediction of the therapy effects by inclusion of different host parameters (morphology, immune state, age, sensibility test) may only approximately be done. In the induction and maintenance of the remission the supportive therapeutic measures play an essential role. By the development of selective cytostatic drugs and further utilisation of the results of molecular-biological experiments further ameliorations of the therapeutic situation are to be awaited.", "PMID": 960899}
{"id": "pubmed23n0032_4105", "title": "[New diagnostic and therapeutic aspects of lymphogranulomatosis].", "content": "The prognosis of lymphogranulomatosis has improved by the considerably improved diagnostics as well as the modern radiological and polychemo-therapeutical methods. Today the average survival time is between 4 and 5 years. But survival times up to 15 years and more are described. Apart from the early diagnosis, into which according to the opinion of many internal specialists the otorhinolaryngologists must be included more than it was hitherto the case, nowadays the modern radiotherapy with explorative laparotomy and splenectomy and the perhaps following combination chemotherapy might bring a further improvement. Growing age, histological types with a small number of lymphocytes, too late diagnosis and deficient control decrease, however, signigicantly the chances of surviving. Certainly, in future more modern methods of chemotherapy must be used. The same applies to the treatment of the developing immunoinsufficiency and the eventual further performance of the cytostatic treatment in the sense of a maintenance therapy. From the modern point of view the way taken might lead to still higher survival times. In as far immunotherapy will be of greater importance already in the next years is not yet to be decided at present.", "contents": "[New diagnostic and therapeutic aspects of lymphogranulomatosis]. The prognosis of lymphogranulomatosis has improved by the considerably improved diagnostics as well as the modern radiological and polychemo-therapeutical methods. Today the average survival time is between 4 and 5 years. But survival times up to 15 years and more are described. Apart from the early diagnosis, into which according to the opinion of many internal specialists the otorhinolaryngologists must be included more than it was hitherto the case, nowadays the modern radiotherapy with explorative laparotomy and splenectomy and the perhaps following combination chemotherapy might bring a further improvement. Growing age, histological types with a small number of lymphocytes, too late diagnosis and deficient control decrease, however, signigicantly the chances of surviving. Certainly, in future more modern methods of chemotherapy must be used. The same applies to the treatment of the developing immunoinsufficiency and the eventual further performance of the cytostatic treatment in the sense of a maintenance therapy. From the modern point of view the way taken might lead to still higher survival times. In as far immunotherapy will be of greater importance already in the next years is not yet to be decided at present.", "PMID": 960900}
{"id": "pubmed23n0032_4106", "title": "[L-alanyl-L-proline-dipeptidase and glycyl-L-valine-depeptidase in malabsorption syndrome].", "content": "On the basis of comparative determinations of the activities of dipeptidases and disaccharidases of the mucous membrane of the small intestine (proximal jejunum) clear correspondences between the morphological findings and the biochemical parameters were the result. L-alanyl-L-prolin-dipeptidase and glycyl-L-valin-dipeptidase as well as lactase, saccharase, maltase and trehalase were determined in altogether 45 children with various malabsorption syndromes of different age in different stages of disease. Diminutions of the activity of the dipeptidases were to be proved analogously to maltase, saccharase and lactase, too, in most cases of subtotal or total villous atrophy. From the results conclusions may be derived to the restricted ability of protein absorption in chronic disease of the small intestine.", "contents": "[L-alanyl-L-proline-dipeptidase and glycyl-L-valine-depeptidase in malabsorption syndrome]. On the basis of comparative determinations of the activities of dipeptidases and disaccharidases of the mucous membrane of the small intestine (proximal jejunum) clear correspondences between the morphological findings and the biochemical parameters were the result. L-alanyl-L-prolin-dipeptidase and glycyl-L-valin-dipeptidase as well as lactase, saccharase, maltase and trehalase were determined in altogether 45 children with various malabsorption syndromes of different age in different stages of disease. Diminutions of the activity of the dipeptidases were to be proved analogously to maltase, saccharase and lactase, too, in most cases of subtotal or total villous atrophy. From the results conclusions may be derived to the restricted ability of protein absorption in chronic disease of the small intestine.", "PMID": 960901}
{"id": "pubmed23n0032_4107", "title": "[Necrobiosis lipoidica diabetricorum and serum lipids].", "content": "Necrobiosis lipoidica diabeticorum is briefly described. Its high coincidence with hyperlipoproteinaemia in casuistic reports from the literature as well as in about half of the 22 cases observed in our clinic can be taken in favour of possible relations between these conditions. Disturbances of fat metabolism may even be considered important for pathogenesis of necrobiosis in general, the more as at least no optimally regulated fat and carbohydrate metabolism can be achieved by best therapeutic control of carbohydrate parameters in juvenile diabetics. Microangiopathia diabetica seems to exist from the very beginning of diabetes mellitus and may ne a basic etiologic prerequisite for the development of necrobiosis. Topical conditions of certain body regions are said to take part in final precipitation of necrobiotic spots.", "contents": "[Necrobiosis lipoidica diabetricorum and serum lipids]. Necrobiosis lipoidica diabeticorum is briefly described. Its high coincidence with hyperlipoproteinaemia in casuistic reports from the literature as well as in about half of the 22 cases observed in our clinic can be taken in favour of possible relations between these conditions. Disturbances of fat metabolism may even be considered important for pathogenesis of necrobiosis in general, the more as at least no optimally regulated fat and carbohydrate metabolism can be achieved by best therapeutic control of carbohydrate parameters in juvenile diabetics. Microangiopathia diabetica seems to exist from the very beginning of diabetes mellitus and may ne a basic etiologic prerequisite for the development of necrobiosis. Topical conditions of certain body regions are said to take part in final precipitation of necrobiotic spots.", "PMID": 960902}
{"id": "pubmed23n0032_4108", "title": "[Determination of hepatic indocyanine green (ujoviridine) clearance using the slope-method].", "content": "1. The technique of the functional test of the liver by means of the indocyanine green (ujoviridine) test suggests to express the results of the test as a result of clearance on the basis of the slope-clearance. 2. The analysis of the curve of disappearance of the test substance in the serum could not always be traced back to a simple exponential function in a group of clinically healthy control persons (10 women at the age of 10-24 years). For the better estimation of the slope-clearance, therefore, the rinsing effect of the liver should be preferred by means of the analysis of a composed (twofold) exponential function. 3. In the two investigation methods the results of the clearance values were not symmetrically distributed so that as parameters of estimation at first only the mean values of density are used. 4. According to this only orientating study the values of the ujoviridine clearance are below 720 ml/min, assuming a simply exponential rinsing function, or 500 ml/min, respectively, taking as a basis the composed exponential rinsing function below the mean values of density.", "contents": "[Determination of hepatic indocyanine green (ujoviridine) clearance using the slope-method]. 1. The technique of the functional test of the liver by means of the indocyanine green (ujoviridine) test suggests to express the results of the test as a result of clearance on the basis of the slope-clearance. 2. The analysis of the curve of disappearance of the test substance in the serum could not always be traced back to a simple exponential function in a group of clinically healthy control persons (10 women at the age of 10-24 years). For the better estimation of the slope-clearance, therefore, the rinsing effect of the liver should be preferred by means of the analysis of a composed (twofold) exponential function. 3. In the two investigation methods the results of the clearance values were not symmetrically distributed so that as parameters of estimation at first only the mean values of density are used. 4. According to this only orientating study the values of the ujoviridine clearance are below 720 ml/min, assuming a simply exponential rinsing function, or 500 ml/min, respectively, taking as a basis the composed exponential rinsing function below the mean values of density.", "PMID": 960903}
{"id": "pubmed23n0032_4109", "title": "[Adrenergic processess and partial secretion of the stomach].", "content": "The stimulation of alpha- and beta-adrenergic processes by epinephrine, phenylephrine and isoproterenol increases the hydrochloric acid and gastric juice secretion (as well as the secretion of gastric juice components is decreased under the influence of the alpha-adrenergic blocking agents dehydrobenzperidol and beta-adrenergic blocking agent propranolol. However, propranolol essentially reduces the acid components secretion not affecting greatly the alkaline components secretion of the patients with a high basal secretion. As a result the P/NP-ration is decreased.", "contents": "[Adrenergic processess and partial secretion of the stomach]. The stimulation of alpha- and beta-adrenergic processes by epinephrine, phenylephrine and isoproterenol increases the hydrochloric acid and gastric juice secretion (as well as the secretion of gastric juice components is decreased under the influence of the alpha-adrenergic blocking agents dehydrobenzperidol and beta-adrenergic blocking agent propranolol. However, propranolol essentially reduces the acid components secretion not affecting greatly the alkaline components secretion of the patients with a high basal secretion. As a result the P/NP-ration is decreased.", "PMID": 960904}
{"id": "pubmed23n0032_4110", "title": "[Increased insulin-sensitivity as a 1st indication of Addison's disease in a juvenile diabetic patient].", "content": "Case report on a 25-year-old patient with diabetes mellitus which is difficult to be controlled. Relapsing vomitting, loss of body-weight, retention of substances normally contained in the urine, hyperpotassaemia, hypernatraemia as well as an increased insulin sensitivity render an additionally existing morbus Addison probable. Confirmation by means of the determination of the Porter-Silver chromogenes. It is referred to the apparantly frequently not understood syntropy in diabetes mellitus and Addison's disease. An autoimmunological process is discussed.", "contents": "[Increased insulin-sensitivity as a 1st indication of Addison's disease in a juvenile diabetic patient]. Case report on a 25-year-old patient with diabetes mellitus which is difficult to be controlled. Relapsing vomitting, loss of body-weight, retention of substances normally contained in the urine, hyperpotassaemia, hypernatraemia as well as an increased insulin sensitivity render an additionally existing morbus Addison probable. Confirmation by means of the determination of the Porter-Silver chromogenes. It is referred to the apparantly frequently not understood syntropy in diabetes mellitus and Addison's disease. An autoimmunological process is discussed.", "PMID": 960905}
{"id": "pubmed23n0032_4111", "title": "[Congenital multicystic renal dysplasia in the adult age].", "content": "In the multicystical renal dysplasia we have to do with a congenital malformation due to a developmental disturbance of the bud of the ureter. It is characterized by a conglomerate of cysts, rarely by a single cyst without a functioning renal parenchyma, atresia or complete absence of the ureter belonging to this as well as hypoplasia or atresia of the vascular stump. In childhood the clinical picture is not observed frequently, in adult persons only rarely. Though the case in question is a homogeneous disturbance of the development, in childhood and adult age pretty different findings are the result. In childhood in most cases a palpable tumour caused by a multicystical kidney is to be found, in adult persons often a more distinct formation of a defect occurs, particularly of the ureter as well as a nearly obligate calcification of the walls of the cyst. Often the clinical picture is to be found in combination with other, above all urological congenital anomalies. Two own observations of multicystical renal dysplasia in adult age are reported and in connection with 33 cases of literature they are comparatively investigated.", "contents": "[Congenital multicystic renal dysplasia in the adult age]. In the multicystical renal dysplasia we have to do with a congenital malformation due to a developmental disturbance of the bud of the ureter. It is characterized by a conglomerate of cysts, rarely by a single cyst without a functioning renal parenchyma, atresia or complete absence of the ureter belonging to this as well as hypoplasia or atresia of the vascular stump. In childhood the clinical picture is not observed frequently, in adult persons only rarely. Though the case in question is a homogeneous disturbance of the development, in childhood and adult age pretty different findings are the result. In childhood in most cases a palpable tumour caused by a multicystical kidney is to be found, in adult persons often a more distinct formation of a defect occurs, particularly of the ureter as well as a nearly obligate calcification of the walls of the cyst. Often the clinical picture is to be found in combination with other, above all urological congenital anomalies. Two own observations of multicystical renal dysplasia in adult age are reported and in connection with 33 cases of literature they are comparatively investigated.", "PMID": 960906}
{"id": "pubmed23n0032_4112", "title": "[Endotoxinemia in liver cirrhosis].", "content": "Endotoxins of gram-negative bacteria and of intestinal origin, insufficiently cleared by the hepatic reticulo-endothelial system are of an increasing interest within the pathogenesis of liver diseases. With purpose to obtain data concerning incidence and course of endotoxaemia in patients with liver cirrhosis an unselected group of these patients, sequentially admitted, was investigated by means of the Limulus-gelation test, regarded as most sensitive to endotoxins. At the admittance, 65% of the patients had endotoxaemia, further 14% developed endotoxaemia later. In total 79% of the patients investigated had endotoxaemia.---Bleeding from oesophageal varices was associated with endotoxaemia in 78%, functional renal impairment in 75%, consumption coagulopathy in 81%, encephalopathy in 77% and a pyrogen reaction in 82% of the patients. Regarding the Limulus assay, the dilution technique was more sensitive in detection of free endotoxaemia as opposed to the chloroform extract. It is concluded from the results that endotoxaemia in patients with liver cirrhosis is frequent and has to be viewed as relevant within the pathogeneses of chronic liver diseases.", "contents": "[Endotoxinemia in liver cirrhosis]. Endotoxins of gram-negative bacteria and of intestinal origin, insufficiently cleared by the hepatic reticulo-endothelial system are of an increasing interest within the pathogenesis of liver diseases. With purpose to obtain data concerning incidence and course of endotoxaemia in patients with liver cirrhosis an unselected group of these patients, sequentially admitted, was investigated by means of the Limulus-gelation test, regarded as most sensitive to endotoxins. At the admittance, 65% of the patients had endotoxaemia, further 14% developed endotoxaemia later. In total 79% of the patients investigated had endotoxaemia.---Bleeding from oesophageal varices was associated with endotoxaemia in 78%, functional renal impairment in 75%, consumption coagulopathy in 81%, encephalopathy in 77% and a pyrogen reaction in 82% of the patients. Regarding the Limulus assay, the dilution technique was more sensitive in detection of free endotoxaemia as opposed to the chloroform extract. It is concluded from the results that endotoxaemia in patients with liver cirrhosis is frequent and has to be viewed as relevant within the pathogeneses of chronic liver diseases.", "PMID": 960907}
{"id": "pubmed23n0032_4113", "title": "[The effect of flavonoid compounds (+)-cyanidanol-3 in acute viral hepatitis].", "content": "A double blind study was carried out with 14 pairs of patients suffering from acute virus hepatitis in order to determine the action of (+)-cyanidol-3 (2 g daily, administered orally). Among these few patients no significant differences were observed between the two groups. This also holds true for the liver function test with the exception of total serum bilirubin. In the treated patients hyperbilirubinemia decreased significantly faster than in the untreated group (p smaller than 0.05). The values registered with a daily dosis of 1 g (+)-cyanidol-3 did not differ from those recorded in the groups of untreated patients.", "contents": "[The effect of flavonoid compounds (+)-cyanidanol-3 in acute viral hepatitis]. A double blind study was carried out with 14 pairs of patients suffering from acute virus hepatitis in order to determine the action of (+)-cyanidol-3 (2 g daily, administered orally). Among these few patients no significant differences were observed between the two groups. This also holds true for the liver function test with the exception of total serum bilirubin. In the treated patients hyperbilirubinemia decreased significantly faster than in the untreated group (p smaller than 0.05). The values registered with a daily dosis of 1 g (+)-cyanidol-3 did not differ from those recorded in the groups of untreated patients.", "PMID": 960908}
{"id": "pubmed23n0032_4114", "title": "[Rat jejunal xylose and galactose absorption dependent on the intestinal bacterial flora and after cytostatics administration].", "content": "The influence of the bacterial flora of the intestine and of cytostatic drugs on the intestinal xylose and glucose absorption in the rat is studied by a perfusion technique in vivo. Conventional, germfree and decontaminated animals show no different xylose absorption rates while the galactose absorption is decreased in germfree and decontaminated animals compared to conventional rats. Three days after a single injection of cytostatic drugs the xylose absorption is diminished in conventional and decontaminated animals compared to untreated rats. In germfree rats no effect of cytostatics on the xylose absorption can be proved. The galactose absorption is unchanged after cytostatics in conventional and decontaminated animals, but increased in germfree rats.", "contents": "[Rat jejunal xylose and galactose absorption dependent on the intestinal bacterial flora and after cytostatics administration]. The influence of the bacterial flora of the intestine and of cytostatic drugs on the intestinal xylose and glucose absorption in the rat is studied by a perfusion technique in vivo. Conventional, germfree and decontaminated animals show no different xylose absorption rates while the galactose absorption is decreased in germfree and decontaminated animals compared to conventional rats. Three days after a single injection of cytostatic drugs the xylose absorption is diminished in conventional and decontaminated animals compared to untreated rats. In germfree rats no effect of cytostatics on the xylose absorption can be proved. The galactose absorption is unchanged after cytostatics in conventional and decontaminated animals, but increased in germfree rats.", "PMID": 960909}
{"id": "pubmed23n0032_4115", "title": "[Gastrointestinal hormones and blood circulation in the gastric mucosa].", "content": "The gastrointestinal hormones influence gastric mucosal blood flow in different ways. Gastrin, secretin and pancreocymin increase gastric mucosal blood flow, glucagon, vip and somatostatin decrease it. Motilin has a special position. Given alone motilin improves gastric mucosal blood flow, wheras it reduces gastric mucosal blood flow after previous administration of pentagastrin or histamin.", "contents": "[Gastrointestinal hormones and blood circulation in the gastric mucosa]. The gastrointestinal hormones influence gastric mucosal blood flow in different ways. Gastrin, secretin and pancreocymin increase gastric mucosal blood flow, glucagon, vip and somatostatin decrease it. Motilin has a special position. Given alone motilin improves gastric mucosal blood flow, wheras it reduces gastric mucosal blood flow after previous administration of pentagastrin or histamin.", "PMID": 960910}
{"id": "pubmed23n0032_4116", "title": "[Synthesis of gastrointestinal agents].", "content": "The importance of syntheses of biologically active polypeptides especially of gastrointestinal hormones for medical research, is analyzed; six points, as the main goals of a synthetic approach, are discussed with respect to their special significance and are confirmed by six examples.", "contents": "[Synthesis of gastrointestinal agents]. The importance of syntheses of biologically active polypeptides especially of gastrointestinal hormones for medical research, is analyzed; six points, as the main goals of a synthetic approach, are discussed with respect to their special significance and are confirmed by six examples.", "PMID": 960911}
{"id": "pubmed23n0032_4117", "title": "[Benign diseases of the esophagus. An internist's view].", "content": "The work-up of patients with benign diseases of the esophagus should start with a diagnostic evaluation. This includes a carefully taken history, radiologic and endoscopic examinations and, in suspected reflux disease, recording of intraesophageal pH. For the evaluation of functional troubles esophageal manometry is recommended. Patients with complicated reflux disease and failures of antireflux treatment should be operated upon. In high peptic stenoses, Barrett's syndrome should be carefully looked for. Achalasia is treated by pneumatic dilatations with more than 90% good or satisfactory results. But diffuse esophageal spasm is little responsive to therapy. There is a risk of secondary carcinoma in Barrett's syndrome, achalasia, caustic lesions and Plummer-Vinson syndrome. Therefore these patients should be seen at regular intervals. There is an urgent need for controlled studies evaluating the comparative results of medical and surgical therapy.", "contents": "[Benign diseases of the esophagus. An internist's view]. The work-up of patients with benign diseases of the esophagus should start with a diagnostic evaluation. This includes a carefully taken history, radiologic and endoscopic examinations and, in suspected reflux disease, recording of intraesophageal pH. For the evaluation of functional troubles esophageal manometry is recommended. Patients with complicated reflux disease and failures of antireflux treatment should be operated upon. In high peptic stenoses, Barrett's syndrome should be carefully looked for. Achalasia is treated by pneumatic dilatations with more than 90% good or satisfactory results. But diffuse esophageal spasm is little responsive to therapy. There is a risk of secondary carcinoma in Barrett's syndrome, achalasia, caustic lesions and Plummer-Vinson syndrome. Therefore these patients should be seen at regular intervals. There is an urgent need for controlled studies evaluating the comparative results of medical and surgical therapy.", "PMID": 960912}
{"id": "pubmed23n0032_4118", "title": "[Benign diseases of the esophagus].", "content": "Most benign esophageal affections may be successfully subjected to carefully selected non-invasive but mainly to surgical techniques; special attention is being paid today to refluxcaused esophagitis and its sequelae. They both require a special strategy of anti-reflux measures - sometimes combined with suppression of peptic aggression. Fundoplicatio according to R. Nissen - if necessary complemented by selective vagotomy - to a large extent meets the needs of a surgical technique adapted to this type of disease.", "contents": "[Benign diseases of the esophagus]. Most benign esophageal affections may be successfully subjected to carefully selected non-invasive but mainly to surgical techniques; special attention is being paid today to refluxcaused esophagitis and its sequelae. They both require a special strategy of anti-reflux measures - sometimes combined with suppression of peptic aggression. Fundoplicatio according to R. Nissen - if necessary complemented by selective vagotomy - to a large extent meets the needs of a surgical technique adapted to this type of disease.", "PMID": 960913}
{"id": "pubmed23n0032_4119", "title": "[The lower esophageal sphincter and manometry methods].", "content": "For measuring the closing strength in the lower esophageal sphincter an adequate method of manometrie is necessary. A pressure measuring device is placed into the esophageal occlusion segment and is filled with water under a variably fixed pressure. Thus a radial force tries to open the closed sphincter. The opening pressure can be equated to the closing pressure. Moreover the activity of sphincteric musculature influenced by drugs can be investigated under a constant radial pressure.", "contents": "[The lower esophageal sphincter and manometry methods]. For measuring the closing strength in the lower esophageal sphincter an adequate method of manometrie is necessary. A pressure measuring device is placed into the esophageal occlusion segment and is filled with water under a variably fixed pressure. Thus a radial force tries to open the closed sphincter. The opening pressure can be equated to the closing pressure. Moreover the activity of sphincteric musculature influenced by drugs can be investigated under a constant radial pressure.", "PMID": 960914}
{"id": "pubmed23n0032_4120", "title": "[Gastrointestinal hormones and lower esophageal sphincter].", "content": "In the gastroesophageal closing mechanism there is a digestive sphincter which as yet has only been identified by its function. This sphincter is subject to the action of gastrointestinal hormones; all hormones, except gastrin and possibly motilin have a relaxing effect. This action sets in only following administration of relatively large doses and thus corresponds to pharmacological effects. The physiological effect of gastrin long under discussion could not be proved in these tests - at least not for antral G 17. Whether or to what extent other hormones - perhaps Big gastrin - have an effect on the control of the lower esophageal sphincter under normal conditions remains yet to be determined.", "contents": "[Gastrointestinal hormones and lower esophageal sphincter]. In the gastroesophageal closing mechanism there is a digestive sphincter which as yet has only been identified by its function. This sphincter is subject to the action of gastrointestinal hormones; all hormones, except gastrin and possibly motilin have a relaxing effect. This action sets in only following administration of relatively large doses and thus corresponds to pharmacological effects. The physiological effect of gastrin long under discussion could not be proved in these tests - at least not for antral G 17. Whether or to what extent other hormones - perhaps Big gastrin - have an effect on the control of the lower esophageal sphincter under normal conditions remains yet to be determined.", "PMID": 960915}
{"id": "pubmed23n0032_4121", "title": "[Acute hemorrhage of the upper digestive tract with special reference to stress ulcer. Prevention and surgical therapy].", "content": "For the surgical therapy of an acute stress-ulcer bleeding there are three methods to someones disposal: 1.) the local care of a bleeding source 2.) the partial gastrectomy and 3.) vagotomy with pyloroplasty and local care of a bleeding source and as prophylaxis of an acute stomach bleeding the highly selective vagotomy with or without pyloroplasty. On judging the success of these methods, some has to consider the basal illness with its letality, because it is to differ, wether a patient died postoperative on his basal disease, or on the effect of the operation, or out of deficient control of the situation. Considering such points it comes out clear, that today the vagotomy with a drainage operation is the method of choice, because: 1.) through this intervention patients are tainted less as for instance through a partial gastrectomy, 2.) through a technical faultless done operation, especially through a vagotomy a success concerning a relapse bleeding is guaranteed. Beside that, the highly selective vagotomy without drainage operation presents itself as prophylactic measure, to prevent an acute stomach bleeding in a number of transplanted patients (kidney transplantation). The first experiences with this method show, that a improvement of the results is to be expected.", "contents": "[Acute hemorrhage of the upper digestive tract with special reference to stress ulcer. Prevention and surgical therapy]. For the surgical therapy of an acute stress-ulcer bleeding there are three methods to someones disposal: 1.) the local care of a bleeding source 2.) the partial gastrectomy and 3.) vagotomy with pyloroplasty and local care of a bleeding source and as prophylaxis of an acute stomach bleeding the highly selective vagotomy with or without pyloroplasty. On judging the success of these methods, some has to consider the basal illness with its letality, because it is to differ, wether a patient died postoperative on his basal disease, or on the effect of the operation, or out of deficient control of the situation. Considering such points it comes out clear, that today the vagotomy with a drainage operation is the method of choice, because: 1.) through this intervention patients are tainted less as for instance through a partial gastrectomy, 2.) through a technical faultless done operation, especially through a vagotomy a success concerning a relapse bleeding is guaranteed. Beside that, the highly selective vagotomy without drainage operation presents itself as prophylactic measure, to prevent an acute stomach bleeding in a number of transplanted patients (kidney transplantation). The first experiences with this method show, that a improvement of the results is to be expected.", "PMID": 960916}
{"id": "pubmed23n0032_4122", "title": "[Summary of work session 3: Drug-induced dissolution of gallstones].", "content": "Since the successful dissolution of cholesterol gallstones is depending to a vast degree on the selection of patients put to test, selection of apt patients was to be the center point of the work session. Furthermore therapy and side effects was being discussed. The best method of choosing patients for chenodeoxycholic-acid-(CDC)-therapy is presented by oral, less by intravenous, cholecystography. Normally this examination provides us with a fair judgement on structure of stones, size of stones and total stone volume, factors which are of considerable importance for any therapeutic success. Determination of the lithogenic index and the finding of cholesterol cristals in the bile could be competent criteria of selection, but these methods are far to complicated. These measurements could only be applied for the determination of the minimal maintenance dosage before onset of long lasting therapy after stone dissolution. The toxic side effects of CDC-therapy seem to be of minor degree in human beings. Changes of liver tissue that could be unequivocally traced back to CDC could not be detected, in contrast to animal experiments, where as there seems to be a correlationbetween an often observed elevation of transaminases and the CDC-dosage applied. There is no negative influence upon fat metabolism of the patient. There were found no morphologic or functional disorders of the gastro-intestinal tract due to CDC-treatment with the exception of chologenic diarrhea. We have still to wait for the results of long-term studies.", "contents": "[Summary of work session 3: Drug-induced dissolution of gallstones]. Since the successful dissolution of cholesterol gallstones is depending to a vast degree on the selection of patients put to test, selection of apt patients was to be the center point of the work session. Furthermore therapy and side effects was being discussed. The best method of choosing patients for chenodeoxycholic-acid-(CDC)-therapy is presented by oral, less by intravenous, cholecystography. Normally this examination provides us with a fair judgement on structure of stones, size of stones and total stone volume, factors which are of considerable importance for any therapeutic success. Determination of the lithogenic index and the finding of cholesterol cristals in the bile could be competent criteria of selection, but these methods are far to complicated. These measurements could only be applied for the determination of the minimal maintenance dosage before onset of long lasting therapy after stone dissolution. The toxic side effects of CDC-therapy seem to be of minor degree in human beings. Changes of liver tissue that could be unequivocally traced back to CDC could not be detected, in contrast to animal experiments, where as there seems to be a correlationbetween an often observed elevation of transaminases and the CDC-dosage applied. There is no negative influence upon fat metabolism of the patient. There were found no morphologic or functional disorders of the gastro-intestinal tract due to CDC-treatment with the exception of chologenic diarrhea. We have still to wait for the results of long-term studies.", "PMID": 960917}
{"id": "pubmed23n0032_4123", "title": "[Gastrointestinal hormones].", "content": "Gastrointestinal hormones are considered to be those that are formed in the gastrointestinal tract and there, in physiological concentrations, develop their effects on motility, secretion, trophism, bloodflow and absorption. Structural analysis, synthesis or a high degree of purity after extraction, and its exact demonstration by means of a useful radioimmunoassay, form the basis for the establishment of a polypeptide as a gastrointestinal hormone. To this category belong, at the present time, gastrin, cholecystokinin-pancreozymin (CCK-PZ) and secretin. GIP, VIP, motilin, glucagon and somatostatin are considered likely candidates. The substances gastrin and CCK-PZ, which are structurally related and have a predominantly stimulating effect, and the structurally dissimilar motilin, contrast with the partially or totally inhibiting hormones of the glucagon family, namely, secretin, VIP, glucagon-enteroglucagon, GIP and somatostatin. By the combined action of these hormones with one another and with the autonomic nervous system, the digestive processes are regulated. Disturbances in the formation of these hormones, in particular an overproduction, give rise to disease syndromes that can now be diagnosed and, in part, treated by surgery. The therapeutic application of gastrointestinal hormones has now also become a possibility.", "contents": "[Gastrointestinal hormones]. Gastrointestinal hormones are considered to be those that are formed in the gastrointestinal tract and there, in physiological concentrations, develop their effects on motility, secretion, trophism, bloodflow and absorption. Structural analysis, synthesis or a high degree of purity after extraction, and its exact demonstration by means of a useful radioimmunoassay, form the basis for the establishment of a polypeptide as a gastrointestinal hormone. To this category belong, at the present time, gastrin, cholecystokinin-pancreozymin (CCK-PZ) and secretin. GIP, VIP, motilin, glucagon and somatostatin are considered likely candidates. The substances gastrin and CCK-PZ, which are structurally related and have a predominantly stimulating effect, and the structurally dissimilar motilin, contrast with the partially or totally inhibiting hormones of the glucagon family, namely, secretin, VIP, glucagon-enteroglucagon, GIP and somatostatin. By the combined action of these hormones with one another and with the autonomic nervous system, the digestive processes are regulated. Disturbances in the formation of these hormones, in particular an overproduction, give rise to disease syndromes that can now be diagnosed and, in part, treated by surgery. The therapeutic application of gastrointestinal hormones has now also become a possibility.", "PMID": 960954}
{"id": "pubmed23n0032_4124", "title": "[Gastrin in internal medicine].", "content": "A pathophysiological role of gastrin has been established only in the Zollinger-Ellison-Syndrome. Hypergastrinemia may be found in cases with prenicious anemia, atrophic gastritis and after all forms of vagotomy; furthermore in the excluded antrum syndrome, the short bowel syndrome, in renal insufficiency, pyloric stenoses and after oral or parenteral administration of calcium. The role of gastrin in the pathogenesis of duodenal ulcer is unknown.", "contents": "[Gastrin in internal medicine]. A pathophysiological role of gastrin has been established only in the Zollinger-Ellison-Syndrome. Hypergastrinemia may be found in cases with prenicious anemia, atrophic gastritis and after all forms of vagotomy; furthermore in the excluded antrum syndrome, the short bowel syndrome, in renal insufficiency, pyloric stenoses and after oral or parenteral administration of calcium. The role of gastrin in the pathogenesis of duodenal ulcer is unknown.", "PMID": 960955}
{"id": "pubmed23n0032_4125", "title": "[Gastrin in surgery].", "content": "The clinical symptomatology of the Zollinger-Ellison-syndrome, the diagnostic treatment and the surgical procedure are reviewed. The syndrome with excessive non-regulated gastrin release from the ZE-tumor is the only form of ulcer disease with importance for the surgeon. The development of a sensitive radioimmunoassay for circulating gastrin facilitated its diagnosis. The possibility of Zollinger-Ellison-syndrome should always be considered if ulcer recur after surgical procedure by simple ulcer disease. If ulcers are located at atypical sites, if multiple ulcer are found, the diagnosis of a Zollinger-Ellison-syndrome should be established by measuring serum gastrin levels.", "contents": "[Gastrin in surgery]. The clinical symptomatology of the Zollinger-Ellison-syndrome, the diagnostic treatment and the surgical procedure are reviewed. The syndrome with excessive non-regulated gastrin release from the ZE-tumor is the only form of ulcer disease with importance for the surgeon. The development of a sensitive radioimmunoassay for circulating gastrin facilitated its diagnosis. The possibility of Zollinger-Ellison-syndrome should always be considered if ulcer recur after surgical procedure by simple ulcer disease. If ulcers are located at atypical sites, if multiple ulcer are found, the diagnosis of a Zollinger-Ellison-syndrome should be established by measuring serum gastrin levels.", "PMID": 960956}
{"id": "pubmed23n0032_4126", "title": "[The problem of functional achlorhydria].", "content": "It was examined whether in anatomically intact specific adenic parenchyma of the gastric mucosa achlorhydria can persist after maximal stimulation or whether there is support to the conception of functional achlorhydria (\"functional anacidity\") furthermore. Relating to the anatomic structure of the gastric mucosa secretion of chloric acid was studied after submaximal and maximal stimulation in 101 cases. It was shown that there is no correlation between the morphology and the secretion of chloric acid in anatomically intact gastric mucosa after submaximal stimulation. In 18 cases out of 28 only the secretion of acid was in correspondence to the histological condition. In two cases out of 37 with atrophic gastric mucosa diverging results were found. After maximal stimulation with histamine chloride and pentagastrine one out of two cases with histologically intact mucosa showed normochlorhydria after seven days, whereas the second case remained achlorhydric. These results must be interpreted as functional achlorhydria. Submaximal stimulation of gastric secretion as it is practised today does not allow corresponding consequences concerning the morphological condition of the gastric mucosa. Discrepant results are found especially in histologically normal mucosa. There is more congruence in atrophic gastric mucosa.", "contents": "[The problem of functional achlorhydria]. It was examined whether in anatomically intact specific adenic parenchyma of the gastric mucosa achlorhydria can persist after maximal stimulation or whether there is support to the conception of functional achlorhydria (\"functional anacidity\") furthermore. Relating to the anatomic structure of the gastric mucosa secretion of chloric acid was studied after submaximal and maximal stimulation in 101 cases. It was shown that there is no correlation between the morphology and the secretion of chloric acid in anatomically intact gastric mucosa after submaximal stimulation. In 18 cases out of 28 only the secretion of acid was in correspondence to the histological condition. In two cases out of 37 with atrophic gastric mucosa diverging results were found. After maximal stimulation with histamine chloride and pentagastrine one out of two cases with histologically intact mucosa showed normochlorhydria after seven days, whereas the second case remained achlorhydric. These results must be interpreted as functional achlorhydria. Submaximal stimulation of gastric secretion as it is practised today does not allow corresponding consequences concerning the morphological condition of the gastric mucosa. Discrepant results are found especially in histologically normal mucosa. There is more congruence in atrophic gastric mucosa.", "PMID": 960957}
{"id": "pubmed23n0032_4127", "title": "[Double contrast examination of the stomach. Definition, technic and indications].", "content": "Double contrast examination constitutes an important element in radiological gastric examination. The author examines the history of the term \"double contrast\" and defines the designation of \"double contrast examination\". The exclusive use of the double contrast method reduces the diagnostic value of gastric X-ray examinations. Thus a method is suggested that will avoid the disadvantages of exclusive double contrast examination while adequately including double contrast in the gastric examination. This is done by using various methods - according to the problem posed - in order to achieve the double contrast.", "contents": "[Double contrast examination of the stomach. Definition, technic and indications]. Double contrast examination constitutes an important element in radiological gastric examination. The author examines the history of the term \"double contrast\" and defines the designation of \"double contrast examination\". The exclusive use of the double contrast method reduces the diagnostic value of gastric X-ray examinations. Thus a method is suggested that will avoid the disadvantages of exclusive double contrast examination while adequately including double contrast in the gastric examination. This is done by using various methods - according to the problem posed - in order to achieve the double contrast.", "PMID": 960958}
{"id": "pubmed23n0032_4128", "title": "[Examination using the gastro-camera and gastroscopy--confrontation and complementation].", "content": "Both the gastro-camera and gastroscopy are methods of examination dating back to the last century. Since 1955 examination with the gastro-camera has gained major importance in Japan. Not until the efficient gastroscopic instruments based on fiber optics and with extremely flexible points as well as advance view optical systems were developed large-scale, introduction esophageal gastro-bulboscopy in hospitals and in offices of specialists became possible. In our view the replacement of the gastro-camera method of diagnosis as a basic examination by gastroscopy is not justified. The gastro-camera is highly efficient and easily applied if centers for early diagnosis of gastric tumor cooperate with the examining physicans; their own experience with the gastro-camera is a premise for this arrangement. The principal task of endoscopic diagnosis is the determination as to therapy, namely whether surgery or a conservative method should be applied: this is particularly true in the case of early carcinoma stages where a 90% 5-year survival chance prevails. For various reasons gastro-camera examination should be assigned a more prominent role. Gastroscopy and histological examination represent further steps in diagnosing malignant processes. It is not possible to preclude the possiblity of a malignant tumor by means of gastroscopic biopsy.", "contents": "[Examination using the gastro-camera and gastroscopy--confrontation and complementation]. Both the gastro-camera and gastroscopy are methods of examination dating back to the last century. Since 1955 examination with the gastro-camera has gained major importance in Japan. Not until the efficient gastroscopic instruments based on fiber optics and with extremely flexible points as well as advance view optical systems were developed large-scale, introduction esophageal gastro-bulboscopy in hospitals and in offices of specialists became possible. In our view the replacement of the gastro-camera method of diagnosis as a basic examination by gastroscopy is not justified. The gastro-camera is highly efficient and easily applied if centers for early diagnosis of gastric tumor cooperate with the examining physicans; their own experience with the gastro-camera is a premise for this arrangement. The principal task of endoscopic diagnosis is the determination as to therapy, namely whether surgery or a conservative method should be applied: this is particularly true in the case of early carcinoma stages where a 90% 5-year survival chance prevails. For various reasons gastro-camera examination should be assigned a more prominent role. Gastroscopy and histological examination represent further steps in diagnosing malignant processes. It is not possible to preclude the possiblity of a malignant tumor by means of gastroscopic biopsy.", "PMID": 960959}
{"id": "pubmed23n0032_4129", "title": "[Endoscopic tumor-cell diagnosis within the region of duodenum].", "content": "The technique spontaneously or siphoned after cholecystokinin stimulation. Our technique of cell collection within the region of the duodenum, the pancreas and the bile-ducts have been optimal performed by duodenoscopical methods. From the papilla of Vater, the cell brush abrasion is used. Pancreatic secretions are obtained by praepapillary drainage with a catheter after secretin stimulation or siphoned after canulation of the ductus Wirsungianus. Bile is obtained wth the same technique of material collection and processing is described. Characteristical tumor cell findings of the papilla of Vater, of the pancreas and the bile ducts are demonstrated and the cytodiagnostic's position within the tumor recognition in this area is discussed. We think the tumor cell search with the described duodenoscopical techniques as indicated in following cases: 1. suspicion of carcinoma of papilla of Vater, 2.obstructve jaundice, 3. in search of carcinoma of the pancreas.", "contents": "[Endoscopic tumor-cell diagnosis within the region of duodenum]. The technique spontaneously or siphoned after cholecystokinin stimulation. Our technique of cell collection within the region of the duodenum, the pancreas and the bile-ducts have been optimal performed by duodenoscopical methods. From the papilla of Vater, the cell brush abrasion is used. Pancreatic secretions are obtained by praepapillary drainage with a catheter after secretin stimulation or siphoned after canulation of the ductus Wirsungianus. Bile is obtained wth the same technique of material collection and processing is described. Characteristical tumor cell findings of the papilla of Vater, of the pancreas and the bile ducts are demonstrated and the cytodiagnostic's position within the tumor recognition in this area is discussed. We think the tumor cell search with the described duodenoscopical techniques as indicated in following cases: 1. suspicion of carcinoma of papilla of Vater, 2.obstructve jaundice, 3. in search of carcinoma of the pancreas.", "PMID": 960960}
{"id": "pubmed23n0032_4130", "title": "[Sterilization during puerperium (author's transl)].", "content": "Sterilization of the woman during puerperium is performed immediately following delivery via a periumbilical incision during a caesaren section isthmica intraperitonealis via laparoscopy or 3--5 days following delivery by pelviscopy. The surgical procedure currently in use for ligating or severing the fallopian tubes is accompanied by extensive loss of blood. This procedure should, therefore, be replaced by the coagulation method which is safer and does not contribute to the formation of adhesions. If the abdomen has been dissected, either high frequency current or the Endo-coagulation procedure may be used to produce the destructive heat necessary to coagulate the fallopian tubes. If the abdomen has not been opened and the coagulation of the fallopian tubes must be performed via pelviscopy, the Endo-coagulation method should be used instead of the high frequency current procedure which cannot be absolutely controlled by the surgeon. The Endo-coagulation procedure works at or about the temperature of boiling water. The failure rate for this procedure is about 2--3 %0.", "contents": "[Sterilization during puerperium (author's transl)]. Sterilization of the woman during puerperium is performed immediately following delivery via a periumbilical incision during a caesaren section isthmica intraperitonealis via laparoscopy or 3--5 days following delivery by pelviscopy. The surgical procedure currently in use for ligating or severing the fallopian tubes is accompanied by extensive loss of blood. This procedure should, therefore, be replaced by the coagulation method which is safer and does not contribute to the formation of adhesions. If the abdomen has been dissected, either high frequency current or the Endo-coagulation procedure may be used to produce the destructive heat necessary to coagulate the fallopian tubes. If the abdomen has not been opened and the coagulation of the fallopian tubes must be performed via pelviscopy, the Endo-coagulation method should be used instead of the high frequency current procedure which cannot be absolutely controlled by the surgeon. The Endo-coagulation procedure works at or about the temperature of boiling water. The failure rate for this procedure is about 2--3 %0.", "PMID": 960961}
{"id": "pubmed23n0032_4131", "title": "[Serum concentrations of SP1, HLP and estriol in last trimester of normal pregnancy: circadian variations and influence of labour (author's transl)].", "content": "Serum concentrations of SP1, HPL and estriol were investigated for circadian variations: levels of SP1 were significantly decreased at the 24th and 4th hour. An investigation of serum concentrations of SP1, HPL and estriol during labours revealed a tendency of decreasing levels of estriol.", "contents": "[Serum concentrations of SP1, HLP and estriol in last trimester of normal pregnancy: circadian variations and influence of labour (author's transl)]. Serum concentrations of SP1, HPL and estriol were investigated for circadian variations: levels of SP1 were significantly decreased at the 24th and 4th hour. An investigation of serum concentrations of SP1, HPL and estriol during labours revealed a tendency of decreasing levels of estriol.", "PMID": 960962}
{"id": "pubmed23n0032_4132", "title": "The oxytocin challenge test in the prognosis of high-risk labor.", "content": "In of 707 pregnant women in the last five weeks of pregnancy a total of 1025 Oxytocin-Challenge Tests (OCT) were performed. Perinatal mortality in the toral material was 16,8% crude and 14,2% corrected. The results of the test were classified into three patterns: normal, borderline (or prepathological) and pathological. The main characteristics of these three patterns are described. Normal OCT was found in 84.9% of the tests and 83,7% of the cases. Borderline OCT was present in 8% of the tests and 7,7% of the cases. Finally pathological profiles were present in 7% and 8,4% respectively. The results of the OCT were compared with the perinatal mortality in the three groups and with the percentage of fetal distress intra partum. It is concluded that the OCT is perhaps the most accurate method to ascertain acute placental insufficiency pre partum. Even the more controversial borderline cases, have a clinical significance.", "contents": "The oxytocin challenge test in the prognosis of high-risk labor. In of 707 pregnant women in the last five weeks of pregnancy a total of 1025 Oxytocin-Challenge Tests (OCT) were performed. Perinatal mortality in the toral material was 16,8% crude and 14,2% corrected. The results of the test were classified into three patterns: normal, borderline (or prepathological) and pathological. The main characteristics of these three patterns are described. Normal OCT was found in 84.9% of the tests and 83,7% of the cases. Borderline OCT was present in 8% of the tests and 7,7% of the cases. Finally pathological profiles were present in 7% and 8,4% respectively. The results of the OCT were compared with the perinatal mortality in the three groups and with the percentage of fetal distress intra partum. It is concluded that the OCT is perhaps the most accurate method to ascertain acute placental insufficiency pre partum. Even the more controversial borderline cases, have a clinical significance.", "PMID": 960963}
{"id": "pubmed23n0032_4133", "title": "[Uterine activity in late pregnancy (author's transl)].", "content": "In late pregnancy three different dinks of uterine activity can be observed by external tocography: Alvarez-waves, Braxton-Hicks-contractions and phases of inactivity equivalent to the intervals of labour. Contractions and phases of inactivity increases on to delivery, while the portion of Alvarez-waves decreases. Braxton-Hicks-contractions and phases of inactivity are opposed to the uncoordinated Alvarex-waves as coordinated activity. Accordingly these changes are explained as a process of increasing coordination of uterine activity, which finally ends in delivery.", "contents": "[Uterine activity in late pregnancy (author's transl)]. In late pregnancy three different dinks of uterine activity can be observed by external tocography: Alvarez-waves, Braxton-Hicks-contractions and phases of inactivity equivalent to the intervals of labour. Contractions and phases of inactivity increases on to delivery, while the portion of Alvarez-waves decreases. Braxton-Hicks-contractions and phases of inactivity are opposed to the uncoordinated Alvarex-waves as coordinated activity. Accordingly these changes are explained as a process of increasing coordination of uterine activity, which finally ends in delivery.", "PMID": 960964}
{"id": "pubmed23n0032_4134", "title": "[Gangrenous pyoderma and IgA-paraproteinemia].", "content": "A gammaAchi--paraproteinemia was found in a 73 year-old female patient with pyoderma gangraenosum. Since the paraporteins hitherto observed in pyoderma gangraenosum predominantly belong to the IgA class, a relationship may exist between pyoderma gangraenosum and this type of paraproteinemia.", "contents": "[Gangrenous pyoderma and IgA-paraproteinemia]. A gammaAchi--paraproteinemia was found in a 73 year-old female patient with pyoderma gangraenosum. Since the paraporteins hitherto observed in pyoderma gangraenosum predominantly belong to the IgA class, a relationship may exist between pyoderma gangraenosum and this type of paraproteinemia.", "PMID": 960967}
{"id": "pubmed23n0032_4135", "title": "The in vitro effect of diethylaminoethyl dextran on stimulation of mouse lymphocytes, and on the mitogenic activity of concanavalin A and lipopolysaccharide.", "content": "Unpurified lymphocytes from spleen, lymph nodes, thymus, and T and B cell-enriched fractions of spleen cells from NMRI mice as well as lymphocytes of nude mice were cultured and stimulation of the lymphocytes by DEAE-D, Con A and LPS and combinations thereof was measured by incorporation of 3H-thymidine. It was demonstrated that DEAE-D is a rather weak mitogen for mouse lymphocytes, acting apparently on B cells as well as on T cells. Frequency and intensity of lymphocyte stimulation by the T cell mitogen Con A and the B cell mitogen LPS was much better. When combinations of DEAE-D with Con A or LPS, respectively, were used significant enhancement of lymphocyte stimulation occurred, as compared to the mitogens and DEAE-D alone. This enhancement was more prominent with the DEAE-D/Con A combination than with the DEAE-D/LPS combination, and more pronounced with the T cell-rich lymphocyte preparations than with the B cell-rich suspensions. The results are discussed and it is concluded that DEAE-D enhances both T cell and B cell functions, however, T cell functions are favoured. This enhancement is assumed to be mediated by a membrane effect of DEAE-D, and to be responsible for th immunological adjuvant effect of DEAE-D.", "contents": "The in vitro effect of diethylaminoethyl dextran on stimulation of mouse lymphocytes, and on the mitogenic activity of concanavalin A and lipopolysaccharide. Unpurified lymphocytes from spleen, lymph nodes, thymus, and T and B cell-enriched fractions of spleen cells from NMRI mice as well as lymphocytes of nude mice were cultured and stimulation of the lymphocytes by DEAE-D, Con A and LPS and combinations thereof was measured by incorporation of 3H-thymidine. It was demonstrated that DEAE-D is a rather weak mitogen for mouse lymphocytes, acting apparently on B cells as well as on T cells. Frequency and intensity of lymphocyte stimulation by the T cell mitogen Con A and the B cell mitogen LPS was much better. When combinations of DEAE-D with Con A or LPS, respectively, were used significant enhancement of lymphocyte stimulation occurred, as compared to the mitogens and DEAE-D alone. This enhancement was more prominent with the DEAE-D/Con A combination than with the DEAE-D/LPS combination, and more pronounced with the T cell-rich lymphocyte preparations than with the B cell-rich suspensions. The results are discussed and it is concluded that DEAE-D enhances both T cell and B cell functions, however, T cell functions are favoured. This enhancement is assumed to be mediated by a membrane effect of DEAE-D, and to be responsible for th immunological adjuvant effect of DEAE-D.", "PMID": 960969}
{"id": "pubmed23n0032_4136", "title": "Antigenic relationship between chicken brain and granulocytes.", "content": "Antisera prepared in rabbits and turkeys against chicken brain were tested in membrane immunofluorescence and lymphocytotoxicity tests for their reactivity with thymocytes and cells from the bursa of Fabricius of normal White Leghorn chickens. In contrast to the findings in mice and other mammals, these antisera did not afford any specific reaction with thymus cells after exhaustive absorptions of species specific antibodies with cells from various tissues. However, absorbed rabbit antisera could be shown to react specifically with cells from the granulocytic series in the bone marrow and, to a lesser extent, in the peripheral blood of chickens.", "contents": "Antigenic relationship between chicken brain and granulocytes. Antisera prepared in rabbits and turkeys against chicken brain were tested in membrane immunofluorescence and lymphocytotoxicity tests for their reactivity with thymocytes and cells from the bursa of Fabricius of normal White Leghorn chickens. In contrast to the findings in mice and other mammals, these antisera did not afford any specific reaction with thymus cells after exhaustive absorptions of species specific antibodies with cells from various tissues. However, absorbed rabbit antisera could be shown to react specifically with cells from the granulocytic series in the bone marrow and, to a lesser extent, in the peripheral blood of chickens.", "PMID": 960970}
{"id": "pubmed23n0032_4137", "title": "Genetic differences between endotoxin-sensitive and resistant C3H mice. Intraperitoneal cell response to lipopolysaccharide and prostaglandins.", "content": "The 2 related mouse strains C3H/HeJ and C3/Han differe in their response to endotoxin. C3H/HeJ mice are endotoxin-resistant, C3H/Han mice are - like all other mice - endotoxin-sesinsitive. The uptake of fluorescent intraperitoneally injected lipopolysaccharide was similar in both mouse strains. The intraperitoneal injection of lipopolysaccharide produced a higher mobilization of neutrophil granulocytes in resistant C3H/HeJ mice than in C3H/Han mice. An opposite pattern of mobilization of neutrophil granulocytes could be observed after injection of the prostaglandins E 1, E 2, and F 1 alpha: which both mouse strains were compared, a normal mobilization was observed in C3H/Han mice. C3H/HeJ mice showed a markedly decreased mobilization. If the difference between the 2 strains resides in a single gene locus itis to be assumed that lipopolysaccharide resistance is controlled by the same gene which also effects an impaired response to neutrophil mobilization after prostaglandin application.", "contents": "Genetic differences between endotoxin-sensitive and resistant C3H mice. Intraperitoneal cell response to lipopolysaccharide and prostaglandins. The 2 related mouse strains C3H/HeJ and C3/Han differe in their response to endotoxin. C3H/HeJ mice are endotoxin-resistant, C3H/Han mice are - like all other mice - endotoxin-sesinsitive. The uptake of fluorescent intraperitoneally injected lipopolysaccharide was similar in both mouse strains. The intraperitoneal injection of lipopolysaccharide produced a higher mobilization of neutrophil granulocytes in resistant C3H/HeJ mice than in C3H/Han mice. An opposite pattern of mobilization of neutrophil granulocytes could be observed after injection of the prostaglandins E 1, E 2, and F 1 alpha: which both mouse strains were compared, a normal mobilization was observed in C3H/Han mice. C3H/HeJ mice showed a markedly decreased mobilization. If the difference between the 2 strains resides in a single gene locus itis to be assumed that lipopolysaccharide resistance is controlled by the same gene which also effects an impaired response to neutrophil mobilization after prostaglandin application.", "PMID": 960971}
{"id": "pubmed23n0032_4138", "title": "Factors influencing the phagocytosis-stimulated glucose oxidation in porcine leukocytes.", "content": "The stimulation of the D-(1-14C)glucose and D-(6-14C)glucose metabolism in pig leucocytes during the phagocytosis of bacteria and inert particles was studied. The following results were obtained: 1. The magnitude of phagocytosis-stimulated glucose oxidation is directly related to the nature and number of particles added. 2. Live bacteria stimulate the glucose metabolism to a greater extent than do a similar number of heat-killed organisms. As to the extent of the stimulation the species of bacteria offered for phagocytosis is crucial. 3. After in vitro addition of a lipopolysaccharide a stimulating effect is observed, a depression has been shown after the addition of hydrocortisone.", "contents": "Factors influencing the phagocytosis-stimulated glucose oxidation in porcine leukocytes. The stimulation of the D-(1-14C)glucose and D-(6-14C)glucose metabolism in pig leucocytes during the phagocytosis of bacteria and inert particles was studied. The following results were obtained: 1. The magnitude of phagocytosis-stimulated glucose oxidation is directly related to the nature and number of particles added. 2. Live bacteria stimulate the glucose metabolism to a greater extent than do a similar number of heat-killed organisms. As to the extent of the stimulation the species of bacteria offered for phagocytosis is crucial. 3. After in vitro addition of a lipopolysaccharide a stimulating effect is observed, a depression has been shown after the addition of hydrocortisone.", "PMID": 960972}
{"id": "pubmed23n0032_4139", "title": "[Studies on the mechanism and duration of antibody-mediated immunosuppression (author's transl)].", "content": "The simultaneous injection of either 10(8) or 5 X 10(5) sheep erythrocytes (SE) and an allogeneic anti-SE serum into mice produced not only a suppression of the primary immune response, but, moreover, the secondary immune reaction elicited, either 4, 8, 12, 16 or 30 weeks after the primary antigenic stimulation, was found to be impaired. This was mainly demonstrated by the significantly reduced numbers of 7 S antibody-synthesizing spleen cells. The suppression of the secondary immune responses is hardly compatible with the conception that the antibody-mediated immunosuppression is solely due to an inactivation of the antigenic determinants by the passively administered specific antibody in the periphery of the immune system. This objection against the so-called \"peripheric theory\" is supported by a further finding. When mice primarily immunized by a simultaneous injection of 10(8) SE and anti-SE-serum were treated with 2 X 10(7) SE 24 hours before boostering with 10(8) SE, in order to eliminate a possibly existing residual activity of the passively administered specific antibodies given together with the primary antigenic stimulus, the secondary 7 S response was likewise found to be significantly suppressed. On the basis of these findings it is suggested that besides the \"peripheric mechanism\" a \"central\" effect plays a significant role in the phenomenon of antibody-mediated immunosuppression, this being due to the reversible or irreversible inactivation of immunocompetent precursor cells by the attachment of antigen-antibody-complexes which results in an inhibition of their differentiation into antibody-producing cells.", "contents": "[Studies on the mechanism and duration of antibody-mediated immunosuppression (author's transl)]. The simultaneous injection of either 10(8) or 5 X 10(5) sheep erythrocytes (SE) and an allogeneic anti-SE serum into mice produced not only a suppression of the primary immune response, but, moreover, the secondary immune reaction elicited, either 4, 8, 12, 16 or 30 weeks after the primary antigenic stimulation, was found to be impaired. This was mainly demonstrated by the significantly reduced numbers of 7 S antibody-synthesizing spleen cells. The suppression of the secondary immune responses is hardly compatible with the conception that the antibody-mediated immunosuppression is solely due to an inactivation of the antigenic determinants by the passively administered specific antibody in the periphery of the immune system. This objection against the so-called \"peripheric theory\" is supported by a further finding. When mice primarily immunized by a simultaneous injection of 10(8) SE and anti-SE-serum were treated with 2 X 10(7) SE 24 hours before boostering with 10(8) SE, in order to eliminate a possibly existing residual activity of the passively administered specific antibodies given together with the primary antigenic stimulus, the secondary 7 S response was likewise found to be significantly suppressed. On the basis of these findings it is suggested that besides the \"peripheric mechanism\" a \"central\" effect plays a significant role in the phenomenon of antibody-mediated immunosuppression, this being due to the reversible or irreversible inactivation of immunocompetent precursor cells by the attachment of antigen-antibody-complexes which results in an inhibition of their differentiation into antibody-producing cells.", "PMID": 960973}
{"id": "pubmed23n0032_4140", "title": "Cooperative binding of a complement component to antigen-antibody complexes. III. Complexes containing IgM antibodies.", "content": "The first component of guinea pig complement (C1)2) is bound in a cooperative manner to antigen-antibody complexes containing rabbit IgM antibodies, as was previously shown to be the case for IgG antibodies. The shape of the binding curves is consistent with an allosteric mechanism involving clusters of 10 interacting C1 binding sites. Similar results were obtained with IgM antibodies against an artificial hapten and against a natural constituent of the erythrocyte membrane.", "contents": "Cooperative binding of a complement component to antigen-antibody complexes. III. Complexes containing IgM antibodies. The first component of guinea pig complement (C1)2) is bound in a cooperative manner to antigen-antibody complexes containing rabbit IgM antibodies, as was previously shown to be the case for IgG antibodies. The shape of the binding curves is consistent with an allosteric mechanism involving clusters of 10 interacting C1 binding sites. Similar results were obtained with IgM antibodies against an artificial hapten and against a natural constituent of the erythrocyte membrane.", "PMID": 960974}
{"id": "pubmed23n0032_4141", "title": "[Diagnostic value of mitral and septal echocardiographic changes in aortic valve insufficiency].", "content": "116 patients with aortic regurgitation of different severity with and without associated valvular lesions were studied with echocardiography. In all cases the diagnosis was proven by cardiac catheterisation. Typical fluttering of the anterior mitral valve leaflet and the interventricular septum were oftener observed with increasing degree of severity of aortic regurgitation. An associated fluttering of the posterior mitral valve leaflet is described. This is seen with increasing severity of the valvular lesion and in more than 90% of patients with severe aortic regurgitation as it is observed with the anterior mitral leaflet. An isolated fluttering of the interventricular septum was observed in two cases and is also regarded as a specific finding in aortic regurgitation. There was a significant higher pulse pressure in patients with fluttering of the anterior mitral leaflet and/or the interventricular septum than in those cases without fluttering. Fluttering of mitral leaflets in aortic regurgitation was seen even in patients with additional mitral stenosis with and without calcification and after commissurotomy. A premature mitral valve closure was noted in one of 93 cases, an incomplete diastolic aortic valve closure in 29 of 78 cases. The diastolic diameter of the aortic root was mildly increased in 17 patients with pure aortic regurgitation. In 8 patients, mostly with severe aortic regurgitation, the E-F-Slope of the anterior mitral leaflet ranged between 120 and 160 mm/sec. The presented findings do not allow a conclusion regarding the severity of aortic regurgitation. However, they do allow the echocardiographic diagnosis of aortic regurgitation with a high degree of accuracy, in particular in cases of fluttering.", "contents": "[Diagnostic value of mitral and septal echocardiographic changes in aortic valve insufficiency]. 116 patients with aortic regurgitation of different severity with and without associated valvular lesions were studied with echocardiography. In all cases the diagnosis was proven by cardiac catheterisation. Typical fluttering of the anterior mitral valve leaflet and the interventricular septum were oftener observed with increasing degree of severity of aortic regurgitation. An associated fluttering of the posterior mitral valve leaflet is described. This is seen with increasing severity of the valvular lesion and in more than 90% of patients with severe aortic regurgitation as it is observed with the anterior mitral leaflet. An isolated fluttering of the interventricular septum was observed in two cases and is also regarded as a specific finding in aortic regurgitation. There was a significant higher pulse pressure in patients with fluttering of the anterior mitral leaflet and/or the interventricular septum than in those cases without fluttering. Fluttering of mitral leaflets in aortic regurgitation was seen even in patients with additional mitral stenosis with and without calcification and after commissurotomy. A premature mitral valve closure was noted in one of 93 cases, an incomplete diastolic aortic valve closure in 29 of 78 cases. The diastolic diameter of the aortic root was mildly increased in 17 patients with pure aortic regurgitation. In 8 patients, mostly with severe aortic regurgitation, the E-F-Slope of the anterior mitral leaflet ranged between 120 and 160 mm/sec. The presented findings do not allow a conclusion regarding the severity of aortic regurgitation. However, they do allow the echocardiographic diagnosis of aortic regurgitation with a high degree of accuracy, in particular in cases of fluttering.", "PMID": 960975}
{"id": "pubmed23n0032_4142", "title": "[Autosomal dominant hereditary atrial septal defect with heart conduction defects and mitral valve insufficiency].", "content": "An autosomal dominant inherited ASD with conduction defects in one family is described. 6 members of 4 generations were fallen ill. In 4 patients the diagnosis was made clinically, in 2 sisters the diagnosis was confirmed by operation. Moreover, the last 2 patients had a mitral insufficiency--probably congenital. The ecg-findings of 4 patients additionally showed conduction defects in form of AV-and bundle branch blocks.", "contents": "[Autosomal dominant hereditary atrial septal defect with heart conduction defects and mitral valve insufficiency]. An autosomal dominant inherited ASD with conduction defects in one family is described. 6 members of 4 generations were fallen ill. In 4 patients the diagnosis was made clinically, in 2 sisters the diagnosis was confirmed by operation. Moreover, the last 2 patients had a mitral insufficiency--probably congenital. The ecg-findings of 4 patients additionally showed conduction defects in form of AV-and bundle branch blocks.", "PMID": 960976}
{"id": "pubmed23n0032_4143", "title": "[Severe atrioventricular block in 2 cases with pre-excitation syndrome].", "content": "In two patients with WPW syndrome Type A suffering from syncopes and dizziness intermittent high degree A-V block was observed. The analysis of the surface Ecg revealed in the first case a complete A-V block within the normal conduction system at the level of the A-V node. In the second case there was a constant left bundle branch block with intermittent block in the right fascicle (intermittent trifascicular block). In both cases the preexcitation syndromes could be best explained by accessory tracts bypassing the normal nodal system left side. One-to-one conduction through the bypass occurred only at a distinct range of cycle lengths, at lower frequencies the accessory tracts were refractory and a IInd or IIIrd degree A-V block occurred. However, outside this frequency zone some P waves were conducted through the accessory tracts without changes in cycle lengths. The findings support the thesis of at least two functionally different atrioventricular pathways in patients with preexcitation syndrome.", "contents": "[Severe atrioventricular block in 2 cases with pre-excitation syndrome]. In two patients with WPW syndrome Type A suffering from syncopes and dizziness intermittent high degree A-V block was observed. The analysis of the surface Ecg revealed in the first case a complete A-V block within the normal conduction system at the level of the A-V node. In the second case there was a constant left bundle branch block with intermittent block in the right fascicle (intermittent trifascicular block). In both cases the preexcitation syndromes could be best explained by accessory tracts bypassing the normal nodal system left side. One-to-one conduction through the bypass occurred only at a distinct range of cycle lengths, at lower frequencies the accessory tracts were refractory and a IInd or IIIrd degree A-V block occurred. However, outside this frequency zone some P waves were conducted through the accessory tracts without changes in cycle lengths. The findings support the thesis of at least two functionally different atrioventricular pathways in patients with preexcitation syndrome.", "PMID": 960977}
{"id": "pubmed23n0032_4144", "title": "[Atypical vagal Wenckebach period in a high performance athlete].", "content": "A 27-year-old man, engaged in heavy physical training, exhibited sinusbradyarrhythmia with atrial abnormality and second degree av-block (atypical Wenckebach period). Since abnormal sinusrhythms was established with exercise and following parasympathetic blockade, this rhythm disorder was considered consequential exaggerated vagal tone. With the aid of His-bundle electrocardiography the block was localized in the av-node (AH).", "contents": "[Atypical vagal Wenckebach period in a high performance athlete]. A 27-year-old man, engaged in heavy physical training, exhibited sinusbradyarrhythmia with atrial abnormality and second degree av-block (atypical Wenckebach period). Since abnormal sinusrhythms was established with exercise and following parasympathetic blockade, this rhythm disorder was considered consequential exaggerated vagal tone. With the aid of His-bundle electrocardiography the block was localized in the av-node (AH).", "PMID": 960978}
{"id": "pubmed23n0032_4145", "title": "[Fascicular block and skeletal myopathies].", "content": "Ecg investigation of skeletal myopathies showed in the approximate half of cases mono- and bifascicular blocks (left anterior, left posterior, RBBB). In view of the possibility of development of complete AV-block, whose prognosis will not be the best, cardiological observation is recommended for life.", "contents": "[Fascicular block and skeletal myopathies]. Ecg investigation of skeletal myopathies showed in the approximate half of cases mono- and bifascicular blocks (left anterior, left posterior, RBBB). In view of the possibility of development of complete AV-block, whose prognosis will not be the best, cardiological observation is recommended for life.", "PMID": 960979}
{"id": "pubmed23n0032_4146", "title": "[Effect of somatic parameters on Frank's electrocardiogram in childhood].", "content": "Correlations were computed between 123 different electrocardiographic variables and constitutional parameters of 179 children aged seven to fourteen years using the Frank system. The constitutional parameters consisted of age, body weight, height, chest circumference and deviation from ideal weight related to height. The corrected orthogonal Ecg was automatically measured by a CD 3300 computer using the program of Pipberger. The results show that the greatest number of statistically significant correlations exist between Ecg variables and body height. In contrast to expectations, age takes only the second rank. Normal values of the Frank Ecg should be related to height not to age, at least in the studied age group.", "contents": "[Effect of somatic parameters on Frank's electrocardiogram in childhood]. Correlations were computed between 123 different electrocardiographic variables and constitutional parameters of 179 children aged seven to fourteen years using the Frank system. The constitutional parameters consisted of age, body weight, height, chest circumference and deviation from ideal weight related to height. The corrected orthogonal Ecg was automatically measured by a CD 3300 computer using the program of Pipberger. The results show that the greatest number of statistically significant correlations exist between Ecg variables and body height. In contrast to expectations, age takes only the second rank. Normal values of the Frank Ecg should be related to height not to age, at least in the studied age group.", "PMID": 960980}
{"id": "pubmed23n0032_4147", "title": "[Metabolism of essential and endogenous serum fatty acids in healthy persons, coronary disease and patients with lipid metabolism disorder. Partial aspect of high risk problem patients with age-related lipid and fatty acid concentrations in serum and regulation of long-chain products from essential and endogenous precursors in healthy subjects].", "content": "Total lipid, free and esterified cholesterol and triglycerides significantly increase in concentration with increasing age. Palmitic, palmitoleic, oleic and linoleic acid behave in the same way. Free fatty acids, phospholipids and stearic acid show the same concentrations in all groups of age. C20 fatty acids demonstrate a competitive regulatory mechanism of desaturase activity in their age dependant distribution. While the 20:3(n-9), a product of the endogenous (n-9) family, increases, the 20:3(n-6) and 20:4(n-6), both desaturation and chain elongation products of the essential n-6 family, decrease. Such behavior has been demonstrated in many feeding studies (12, 13, 15, 16, 17, 29) and is interpreted as a result of competitive substrate interaction on desaturase activity.", "contents": "[Metabolism of essential and endogenous serum fatty acids in healthy persons, coronary disease and patients with lipid metabolism disorder. Partial aspect of high risk problem patients with age-related lipid and fatty acid concentrations in serum and regulation of long-chain products from essential and endogenous precursors in healthy subjects]. Total lipid, free and esterified cholesterol and triglycerides significantly increase in concentration with increasing age. Palmitic, palmitoleic, oleic and linoleic acid behave in the same way. Free fatty acids, phospholipids and stearic acid show the same concentrations in all groups of age. C20 fatty acids demonstrate a competitive regulatory mechanism of desaturase activity in their age dependant distribution. While the 20:3(n-9), a product of the endogenous (n-9) family, increases, the 20:3(n-6) and 20:4(n-6), both desaturation and chain elongation products of the essential n-6 family, decrease. Such behavior has been demonstrated in many feeding studies (12, 13, 15, 16, 17, 29) and is interpreted as a result of competitive substrate interaction on desaturase activity.", "PMID": 960981}
{"id": "pubmed23n0032_4148", "title": "[Working method of the Hannover program].", "content": "A view about the operation of the Hanover-Ecg-Program System is given. An essential, if not the most important mark of this system is the combined graphical and numerical print of the results. This print allows the physician a quick preliminary diagnosis and a control of quality. The rhythm diagnostic of the Hanover System is a remarkable progress. Also complicated arrhythms can be recognized in this manner. The rhythm diagram gives the physician a good control.", "contents": "[Working method of the Hannover program]. A view about the operation of the Hanover-Ecg-Program System is given. An essential, if not the most important mark of this system is the combined graphical and numerical print of the results. This print allows the physician a quick preliminary diagnosis and a control of quality. The rhythm diagnostic of the Hanover System is a remarkable progress. Also complicated arrhythms can be recognized in this manner. The rhythm diagram gives the physician a good control.", "PMID": 960982}
{"id": "pubmed23n0032_4149", "title": "An electron microscopic and cytochemical detection of concanavalin A receptors on the cell membrane of Leishmania braziliensis guyanensis.", "content": "Promastigotes of Leishmania braziliensis quyanensis cultivated in the NNN medium agglutinate with concanavalin A (con A). The protozoon was agglutinated at different concentrations of con A. Maximal agglutination was obtained with 150 mug/ml. Three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. Cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. A con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect con A receptors on the cell membrane of the parasite.", "contents": "An electron microscopic and cytochemical detection of concanavalin A receptors on the cell membrane of Leishmania braziliensis guyanensis. Promastigotes of Leishmania braziliensis quyanensis cultivated in the NNN medium agglutinate with concanavalin A (con A). The protozoon was agglutinated at different concentrations of con A. Maximal agglutination was obtained with 150 mug/ml. Three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. Cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. A con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect con A receptors on the cell membrane of the parasite.", "PMID": 961000}
{"id": "pubmed23n0032_4150", "title": "Studies on the ultrastructure, histochemistry and cytochemistry of the uninfected digestive gland of Bithynia tentaculata (Mollusca: Gastropoda) and on the ultrastructure of this host organ in snails infected with larval digeneans.", "content": "The structure and function of the digestive gland of the gastropod mollusc, Bithynia tentaculata, was investigated using ultrastructural, histochemical, and cytochemical techniques. The digestive gland was shown to be composed of two main cell types, the \"digestive\" cells and \"secretory\" cells. The digestive cells appeared to be concerned with the absorption and digestion of nutrients, while secretory cells produced digestive enzymes and calcareous concretions. Undifferentiated cells were scattered between these two main cell types. The pathological effects of larval digeneans on the digestive gland were also investigated, at the ultrastructural level. In such infected snails the digestive gland appeared to be degenerating. The significance of this tissue destruction was briefly discussed.", "contents": "Studies on the ultrastructure, histochemistry and cytochemistry of the uninfected digestive gland of Bithynia tentaculata (Mollusca: Gastropoda) and on the ultrastructure of this host organ in snails infected with larval digeneans. The structure and function of the digestive gland of the gastropod mollusc, Bithynia tentaculata, was investigated using ultrastructural, histochemical, and cytochemical techniques. The digestive gland was shown to be composed of two main cell types, the \"digestive\" cells and \"secretory\" cells. The digestive cells appeared to be concerned with the absorption and digestion of nutrients, while secretory cells produced digestive enzymes and calcareous concretions. Undifferentiated cells were scattered between these two main cell types. The pathological effects of larval digeneans on the digestive gland were also investigated, at the ultrastructural level. In such infected snails the digestive gland appeared to be degenerating. The significance of this tissue destruction was briefly discussed.", "PMID": 961001}
{"id": "pubmed23n0032_4151", "title": "Qualitative analysis of substances excreted by Isoparorchis hypselobagri (Trematoda) during aerobic in vitro culture.", "content": "Isoparorchis hypselobagri from the swim bladder of the catfish, Wallago attu was aerobically cultured in vitro. The dialyzed incubate was microchemically analysed for either individual or group of compounds excreted by the parasite. Amino acids, amines, amides, ammonia, aldehyde, glycine, alpha-alanine, tyrosine, alpha-aminocarboxylic acids, haemoglobin, nitrogen, reducing materials, and lactic, pyruvic, succinic, propionic and acetic acids were found in the incubate, but acetaldehyde, formaldehyde, O-dioxo and oxomethylene, and formic acid were not detected.", "contents": "Qualitative analysis of substances excreted by Isoparorchis hypselobagri (Trematoda) during aerobic in vitro culture. Isoparorchis hypselobagri from the swim bladder of the catfish, Wallago attu was aerobically cultured in vitro. The dialyzed incubate was microchemically analysed for either individual or group of compounds excreted by the parasite. Amino acids, amines, amides, ammonia, aldehyde, glycine, alpha-alanine, tyrosine, alpha-aminocarboxylic acids, haemoglobin, nitrogen, reducing materials, and lactic, pyruvic, succinic, propionic and acetic acids were found in the incubate, but acetaldehyde, formaldehyde, O-dioxo and oxomethylene, and formic acid were not detected.", "PMID": 961002}
{"id": "pubmed23n0032_4152", "title": "Studies on the infectivity of Fasciola hepatica miracidia to Lymnaea truncatula. Attachment and penetration of miracidia into non-infected and infected snails.", "content": "Fasciola hepatica miracidia labelled in vivo with radioselenium have been used in studies on the capacity of the miracidia to attach to and penetrate the tegument of infected compared with non-infected host snails (Lymnaea truncatula). The results show that the penetration of the larvae into the snail tissue is not influenced by an already existing infection, regardless of its developmental stage.", "contents": "Studies on the infectivity of Fasciola hepatica miracidia to Lymnaea truncatula. Attachment and penetration of miracidia into non-infected and infected snails. Fasciola hepatica miracidia labelled in vivo with radioselenium have been used in studies on the capacity of the miracidia to attach to and penetrate the tegument of infected compared with non-infected host snails (Lymnaea truncatula). The results show that the penetration of the larvae into the snail tissue is not influenced by an already existing infection, regardless of its developmental stage.", "PMID": 961003}
{"id": "pubmed23n0032_4153", "title": "Studies on host-flea relationship. IV. Progesterone and cortisone do not influence the reproductive potentials of rat fleas Xenopsylla cheopis (Rothschild) and X. astia (Rothschild).", "content": "Experiments carried out to study the influence of progesterone and cortisone on the initiation of ovarian maturation, fecundity rate and fertility of the two species of rat fleas Xenopsylla cheopis (Rothschild) and X. astia (Rothschild) indicated that these hormones do not exert any influence on their reproductive potentials. This along with earlier observations show that the factors which regulate ovarian maturation and fecundity in these rat fleas are different from those of the rabbit flea Spilopsyllus cuniculi (Dale).", "contents": "Studies on host-flea relationship. IV. Progesterone and cortisone do not influence the reproductive potentials of rat fleas Xenopsylla cheopis (Rothschild) and X. astia (Rothschild). Experiments carried out to study the influence of progesterone and cortisone on the initiation of ovarian maturation, fecundity rate and fertility of the two species of rat fleas Xenopsylla cheopis (Rothschild) and X. astia (Rothschild) indicated that these hormones do not exert any influence on their reproductive potentials. This along with earlier observations show that the factors which regulate ovarian maturation and fecundity in these rat fleas are different from those of the rabbit flea Spilopsyllus cuniculi (Dale).", "PMID": 961004}
{"id": "pubmed23n0032_4154", "title": "[About a substance with antithrombinic activity, found in the latero-oesophageal glands of Meinertia oestroides (Risso, 1826) (Isopoda, Flabellifera, Cymothoidae; parasite of fishes) (author's transl)].", "content": "An anticlotting factor was found recently in the cephalothoracic latero-oesophageal glands (G.L.O.) of Cymothoid Isopods (blood-sucking fishparasites). This substance is efficient on the blood of hosts such as Boops boops (L.), and also on the human plasma. The way of action and the chemical kind of this factor were investigated: Plasmatic coagulation-tests (Howell, Quick, Cephalin-kaolin, Stypven, Stypven-cephalin, Thrombin and Thrombin coagulase times) were realized on citrated plasmas of Boops and man; enzymatic activities of latero-esophageal glands and hepatopancreas of Meinertia oestroides (Risso, 1826) were also studied. Our results show that the anticlotting action of G.L.O. extracts proceeds from an anti-thrombinic factor, certainly carbohydrate-like as heparin.", "contents": "[About a substance with antithrombinic activity, found in the latero-oesophageal glands of Meinertia oestroides (Risso, 1826) (Isopoda, Flabellifera, Cymothoidae; parasite of fishes) (author's transl)]. An anticlotting factor was found recently in the cephalothoracic latero-oesophageal glands (G.L.O.) of Cymothoid Isopods (blood-sucking fishparasites). This substance is efficient on the blood of hosts such as Boops boops (L.), and also on the human plasma. The way of action and the chemical kind of this factor were investigated: Plasmatic coagulation-tests (Howell, Quick, Cephalin-kaolin, Stypven, Stypven-cephalin, Thrombin and Thrombin coagulase times) were realized on citrated plasmas of Boops and man; enzymatic activities of latero-esophageal glands and hepatopancreas of Meinertia oestroides (Risso, 1826) were also studied. Our results show that the anticlotting action of G.L.O. extracts proceeds from an anti-thrombinic factor, certainly carbohydrate-like as heparin.", "PMID": 961005}
{"id": "pubmed23n0032_4155", "title": "Babesia hylomysci in mice: Preference for erythrocytes of a particular age-group and pathogenesis of the anaemia.", "content": "Scintillation counting on 59Fe-labelled haemoglobin in erythrocytes, serum and urine during B. hylomysci infection in mice demonstrated that the parasite had a predilection to older mature erythrocytes and that the anaemia produced during the infection was mainly due to the direct destruction of infected erythrocytes. An autoimmune reaction has also been incriminated as a factor in the pathogenesis of the anaemia, but was of a lesser magnitude and its manifestations were masked by the direct destruction of infected erythrocytes.", "contents": "Babesia hylomysci in mice: Preference for erythrocytes of a particular age-group and pathogenesis of the anaemia. Scintillation counting on 59Fe-labelled haemoglobin in erythrocytes, serum and urine during B. hylomysci infection in mice demonstrated that the parasite had a predilection to older mature erythrocytes and that the anaemia produced during the infection was mainly due to the direct destruction of infected erythrocytes. An autoimmune reaction has also been incriminated as a factor in the pathogenesis of the anaemia, but was of a lesser magnitude and its manifestations were masked by the direct destruction of infected erythrocytes.", "PMID": 961007}
{"id": "pubmed23n0032_4156", "title": "Ultrastructure of macrogametogenesis of Eimeria mivati.", "content": "The development of the macrogamete of Eimeria mivati Edgar and Seibold 1964 was studied with the electron microscope. Development of the young gamont was characterized by a loss of organelles such as the apical complex, subpellicular microtubules, rhoptries and micronemes, followed by an increase in micropores, mitochondria, rough endoplasmic reticulum (rER), and Golgi complexes. Nuclear detachment bodies and canaliculi were present in maturing macrogamonts. Amylopectin was first observed as small electron-dense rod-like bodies that eventually became large electron-transparent bodies. Type II wall-forming bodies developed in the cisternae of the rER. Type I wall-forming bodies appeared shortly thereafter in close association with numerous Golgi complexes. Many small vesicles located between the cisternae of the rER and the Golgi complexes formed what appeared to be a secretory pathway whereby protein formed in the cisternae and, modified by the Golgi complex, may produce the type I wall body material. The outer wall of the oocyst developed between two distal membranes on the surface of the macrogamete. Although the actual mechanism of deposition of the wall material was not seen, it was probably by some secretory process. Wall-forming bodies did not fuse.", "contents": "Ultrastructure of macrogametogenesis of Eimeria mivati. The development of the macrogamete of Eimeria mivati Edgar and Seibold 1964 was studied with the electron microscope. Development of the young gamont was characterized by a loss of organelles such as the apical complex, subpellicular microtubules, rhoptries and micronemes, followed by an increase in micropores, mitochondria, rough endoplasmic reticulum (rER), and Golgi complexes. Nuclear detachment bodies and canaliculi were present in maturing macrogamonts. Amylopectin was first observed as small electron-dense rod-like bodies that eventually became large electron-transparent bodies. Type II wall-forming bodies developed in the cisternae of the rER. Type I wall-forming bodies appeared shortly thereafter in close association with numerous Golgi complexes. Many small vesicles located between the cisternae of the rER and the Golgi complexes formed what appeared to be a secretory pathway whereby protein formed in the cisternae and, modified by the Golgi complex, may produce the type I wall body material. The outer wall of the oocyst developed between two distal membranes on the surface of the macrogamete. Although the actual mechanism of deposition of the wall material was not seen, it was probably by some secretory process. Wall-forming bodies did not fuse.", "PMID": 961008}
{"id": "pubmed23n0032_4157", "title": "Qualitative distribution of neutral lipids and phospholipids in Hymenolepis microstoma from the cysticercoid to the egg producing adult.", "content": "The distribution of neutral lipids and phospholipids in Hymenolepis microstoma has been studied using Fettrot, Sudan Black B, Sudan IV and copper phthalocyanin staining techniques. In the cysticercoid, neutral lipids are found in the outer membrane, the lining of the cysticercoid cavity, the tegument of the larval worm and the calcareous corpuscles. A decreasing gradient of phospholipids is found starting from the acellular layer, through the circular fibrous layer, the longitudinal fibrous layer, the adjacent dense zone and ending with the lining of the cysticercoid cavity. Phospholipids are also found in the calcareous corpuscles and the tegument of the larval worm. In the young adult (3 days p.i.) fat globules are first seen to accumulate in the last 2-3 proglottids. Until the 6th day p.i. they are found in the posterior third of the worm, surrounding developing gonads, but mostly concentrated along the transverse line. The mature proglottids contain fat, (a) in both granular and globular forms: in the folds of the uterus, sperm ducts, cirrus pouch and tegument (proximal cytoplasm), (b) in a diffuse form: in the vitellaria, ovary, testes and the tegument (distal cytoplasm). Pre-gravid and gravid proglottids show the largest fat globules. From the cleaving embryo to the fully developed oncosphere the concentrations of neutral lipids and phospholipids vary in form, intensity and location. In all strobilar forms of the parasite neutral lipids and phospholipids are found in the tegument and calcareous corpuscles. Although in H. microstoma lipid droplets are found in the excretory canals, all lipids in the proglottids are not absolutely waste products. From the results it would appear that they play a role in the maturation of gonads and transformation of the fertilized ovum to the oncosphere.", "contents": "Qualitative distribution of neutral lipids and phospholipids in Hymenolepis microstoma from the cysticercoid to the egg producing adult. The distribution of neutral lipids and phospholipids in Hymenolepis microstoma has been studied using Fettrot, Sudan Black B, Sudan IV and copper phthalocyanin staining techniques. In the cysticercoid, neutral lipids are found in the outer membrane, the lining of the cysticercoid cavity, the tegument of the larval worm and the calcareous corpuscles. A decreasing gradient of phospholipids is found starting from the acellular layer, through the circular fibrous layer, the longitudinal fibrous layer, the adjacent dense zone and ending with the lining of the cysticercoid cavity. Phospholipids are also found in the calcareous corpuscles and the tegument of the larval worm. In the young adult (3 days p.i.) fat globules are first seen to accumulate in the last 2-3 proglottids. Until the 6th day p.i. they are found in the posterior third of the worm, surrounding developing gonads, but mostly concentrated along the transverse line. The mature proglottids contain fat, (a) in both granular and globular forms: in the folds of the uterus, sperm ducts, cirrus pouch and tegument (proximal cytoplasm), (b) in a diffuse form: in the vitellaria, ovary, testes and the tegument (distal cytoplasm). Pre-gravid and gravid proglottids show the largest fat globules. From the cleaving embryo to the fully developed oncosphere the concentrations of neutral lipids and phospholipids vary in form, intensity and location. In all strobilar forms of the parasite neutral lipids and phospholipids are found in the tegument and calcareous corpuscles. Although in H. microstoma lipid droplets are found in the excretory canals, all lipids in the proglottids are not absolutely waste products. From the results it would appear that they play a role in the maturation of gonads and transformation of the fertilized ovum to the oncosphere.", "PMID": 961009}
{"id": "pubmed23n0032_4158", "title": "Jugular venous pulse recording in a patient without a tricuspid valve.", "content": "The unusual contour of the jugular venous pulse tracing (JVPT) in a patient whose tricuspid valve was excised is described. The appearance of an early positive systolic wave (SW) followed by a prominent \"V\" wave and a delayed deep \"Y\" descent were noted.", "contents": "Jugular venous pulse recording in a patient without a tricuspid valve. The unusual contour of the jugular venous pulse tracing (JVPT) in a patient whose tricuspid valve was excised is described. The appearance of an early positive systolic wave (SW) followed by a prominent \"V\" wave and a delayed deep \"Y\" descent were noted.", "PMID": 961030}
{"id": "pubmed23n0032_4159", "title": "Postoperative arrhythmias in open-heart surgery, A study on fifty cases.", "content": "50 consecutive patients undergone open heart surgery were analyzed regarding postoperative arrhythmias in the first postoperative 3 days. Disturbances of rhythm occurred in each case of our group, serious or not serious (100%). Ventricular premature beats were the most frequent type of arrhythmia in the first and second postoperative days (80%). Two cases expired postoperatively. In one of them complete atrioventricular block developed after double valvular replacements (mitral and tricuspid). The other died of low cardiac output syndrome. Etiology of the arrhythmias after open heart surgery is not clear in the absence of electrolyte and metabolic disturbances, digitalis intoxication and surgical trauma to the conduction system and coronary arteries. Low cardiac output syndrome, hypotension and hypoxia can also be blamed in the formation of these arrhythmias. Other factors such as the prolongation of anoxic arrest, irritation of the ventricular septum by valvular prostheses, cardiac irritation by thoracic tubes, psychologic trauma, halothane anesthesia and coronary arterial disease in the old age group may be the possible predisposing factors in these patients.", "contents": "Postoperative arrhythmias in open-heart surgery, A study on fifty cases. 50 consecutive patients undergone open heart surgery were analyzed regarding postoperative arrhythmias in the first postoperative 3 days. Disturbances of rhythm occurred in each case of our group, serious or not serious (100%). Ventricular premature beats were the most frequent type of arrhythmia in the first and second postoperative days (80%). Two cases expired postoperatively. In one of them complete atrioventricular block developed after double valvular replacements (mitral and tricuspid). The other died of low cardiac output syndrome. Etiology of the arrhythmias after open heart surgery is not clear in the absence of electrolyte and metabolic disturbances, digitalis intoxication and surgical trauma to the conduction system and coronary arteries. Low cardiac output syndrome, hypotension and hypoxia can also be blamed in the formation of these arrhythmias. Other factors such as the prolongation of anoxic arrest, irritation of the ventricular septum by valvular prostheses, cardiac irritation by thoracic tubes, psychologic trauma, halothane anesthesia and coronary arterial disease in the old age group may be the possible predisposing factors in these patients.", "PMID": 961031}
{"id": "pubmed23n0032_4160", "title": "Celiac artery obstruction after arteriography.", "content": "The first case of celiac artery obstruction due to selective arteriography is reported. Impairment of flow to liver and duodenum was present; but the patient recovered uneventfully with non-operative treatment. Management of this problem centers around observation, liver support, and putting the gut at rest. If non-operative treatment is failing, operation should be done, vascular reconstruction carried out, and the duodenum inspected to ensure its viability.", "contents": "Celiac artery obstruction after arteriography. The first case of celiac artery obstruction due to selective arteriography is reported. Impairment of flow to liver and duodenum was present; but the patient recovered uneventfully with non-operative treatment. Management of this problem centers around observation, liver support, and putting the gut at rest. If non-operative treatment is failing, operation should be done, vascular reconstruction carried out, and the duodenum inspected to ensure its viability.", "PMID": 961032}
{"id": "pubmed23n0032_4161", "title": "Axillary sympathectomy for upper extremities.", "content": "Among the several various surgical approaches to the cervico-dorsal sympathetic system, the axillary approach seems to us the operation of choice. The operation is simple, gives excellent access to the required sympathetic ganglions, including the lower part of the stellate ganglion, down to the fifth thoracic ganglion. In all cases the sympathectomy was clinically complete. The postoperative course was mostly smooth, and the few cases of transient Horner's Syndrome, pneumothorax and hemothorax could have been avoided.", "contents": "Axillary sympathectomy for upper extremities. Among the several various surgical approaches to the cervico-dorsal sympathetic system, the axillary approach seems to us the operation of choice. The operation is simple, gives excellent access to the required sympathetic ganglions, including the lower part of the stellate ganglion, down to the fifth thoracic ganglion. In all cases the sympathectomy was clinically complete. The postoperative course was mostly smooth, and the few cases of transient Horner's Syndrome, pneumothorax and hemothorax could have been avoided.", "PMID": 961038}
{"id": "pubmed23n0032_4162", "title": "Complications encountered during arterial embolectomy with the Fogarty balloon catheter.", "content": "The Fogarty arterial embolectomy catheter, while indispensible in the armamentarium of the vascular surgeon, is not entirely benign instrument. A case is desribed in which the balloon was lost in its entirety from the catheter and immediately retrieved using a second identical instrument. A comprehensive survey of the literature reveals that a variety of arterial injuries have occurred during the use of the Fogarty catheter. Each of the major ones is discussed in depth. Amongst the most serious are arterial perforation and rupture occasionally followed by loss of the involved extremity. All previously reported complications following use of the Fogarty catheter are tabulated and reviewed. Additionally, a formal classification of these complications is proposed. Since the time of its introduction in 1963 the Fogarty ballon-tipped catheter has become an indispensible tool in the armamentarium of the vascular surgeon. Its use for arterial embolectomy has been responsible for the salvage of many thousands of limbs. Over the course of the past decade, however, a number of complications referable to this instrument have appeared in the literature. These include perforation of vessels, intimal disruption and foreign body embolization amongst others. To our knowledge, however, there has been only one reported case of a balloon having been lost intra-arterially in toto without obvious cause. It is the purpose of this paper to present the second such case where the balloon, which had separated entirely from the catheter during the course of an arterial embolectomy, was later retrieved by passage of a second Fogarty catheter. In addition, a comprehensive review of the literature is undertaken, and all arterial complications reported to date summarized and tabulated.", "contents": "Complications encountered during arterial embolectomy with the Fogarty balloon catheter. The Fogarty arterial embolectomy catheter, while indispensible in the armamentarium of the vascular surgeon, is not entirely benign instrument. A case is desribed in which the balloon was lost in its entirety from the catheter and immediately retrieved using a second identical instrument. A comprehensive survey of the literature reveals that a variety of arterial injuries have occurred during the use of the Fogarty catheter. Each of the major ones is discussed in depth. Amongst the most serious are arterial perforation and rupture occasionally followed by loss of the involved extremity. All previously reported complications following use of the Fogarty catheter are tabulated and reviewed. Additionally, a formal classification of these complications is proposed. Since the time of its introduction in 1963 the Fogarty ballon-tipped catheter has become an indispensible tool in the armamentarium of the vascular surgeon. Its use for arterial embolectomy has been responsible for the salvage of many thousands of limbs. Over the course of the past decade, however, a number of complications referable to this instrument have appeared in the literature. These include perforation of vessels, intimal disruption and foreign body embolization amongst others. To our knowledge, however, there has been only one reported case of a balloon having been lost intra-arterially in toto without obvious cause. It is the purpose of this paper to present the second such case where the balloon, which had separated entirely from the catheter during the course of an arterial embolectomy, was later retrieved by passage of a second Fogarty catheter. In addition, a comprehensive review of the literature is undertaken, and all arterial complications reported to date summarized and tabulated.", "PMID": 961039}
{"id": "pubmed23n0032_4163", "title": "Major peripheral veins injuries.", "content": "The injury was severe in wounded limb patients of this series who suffered from an associated major peripheral vein trauma. The presence of such an injury weighed heavily on the prognosis. Thirty eight patients with major peripheral veins injuries are reviewed. The injury had resulted from war wounds, work or road accidents. The superficial femoral vein was the most frequently injured vein. Associated injuries were frequently noted: soft tissues injuries in 35 patients, fractures in 33, arterial injuries in 32 and peripheral nerve injuries in 22 patients. Shock was more often present and more severe in patients who suffered also from a vein injury than in patients with an arterial injury only. The lacerated femoral vein was ligated in the majority of patients. Attempts were made to repair the lacerated popliteal veins. Repair of the vein was usually done by anastomosis of debrided ends. When an arterial injury was also present, it was repaired first. The postoperative complications were frequent and included infections, thrombophlebitis and pulmonary embolisations. Twenty four patients were discharged with a viable limb. Complete function was recovered in seven patients only. Partial neurological deficit remained in twelve patients and complete paralysis in five. Twelve patients underwent subsequent amputation of the injured limb. Indications for amputation were ischemia in eight, infection or extensive destruction of tissues in four. Two patients died.", "contents": "Major peripheral veins injuries. The injury was severe in wounded limb patients of this series who suffered from an associated major peripheral vein trauma. The presence of such an injury weighed heavily on the prognosis. Thirty eight patients with major peripheral veins injuries are reviewed. The injury had resulted from war wounds, work or road accidents. The superficial femoral vein was the most frequently injured vein. Associated injuries were frequently noted: soft tissues injuries in 35 patients, fractures in 33, arterial injuries in 32 and peripheral nerve injuries in 22 patients. Shock was more often present and more severe in patients who suffered also from a vein injury than in patients with an arterial injury only. The lacerated femoral vein was ligated in the majority of patients. Attempts were made to repair the lacerated popliteal veins. Repair of the vein was usually done by anastomosis of debrided ends. When an arterial injury was also present, it was repaired first. The postoperative complications were frequent and included infections, thrombophlebitis and pulmonary embolisations. Twenty four patients were discharged with a viable limb. Complete function was recovered in seven patients only. Partial neurological deficit remained in twelve patients and complete paralysis in five. Twelve patients underwent subsequent amputation of the injured limb. Indications for amputation were ischemia in eight, infection or extensive destruction of tissues in four. Two patients died.", "PMID": 961040}
{"id": "pubmed23n0032_4164", "title": "Bovine artegraft arteriovenous fistulas for hemodialysis in one-hundred patients after \"conventional\" arteriovenous fistulas failed.", "content": "The use of the Bovine artegraft for creation of arteriovenous fistulas in the hemodialysis patients was first carried out in the midsummer of 1971. Since that time, there has been considerable material reported in the use of this substitute as an access for hemodialysis. In this article information gained from an ongoing study of the first 100 patients in chronic hemodialysis who had Bovine artegraft arteriovenous fistulas was reviewed. The indications for operation, the standard technique, the results, the complications, and the future plans for study of the physiology and hemodynamics of this type of fistula are described. The Bovine artegraft, AV fistula can be created successfully in a high percentage of patients, offers ease of needle placement, and effective dialysis with very little complication.", "contents": "Bovine artegraft arteriovenous fistulas for hemodialysis in one-hundred patients after \"conventional\" arteriovenous fistulas failed. The use of the Bovine artegraft for creation of arteriovenous fistulas in the hemodialysis patients was first carried out in the midsummer of 1971. Since that time, there has been considerable material reported in the use of this substitute as an access for hemodialysis. In this article information gained from an ongoing study of the first 100 patients in chronic hemodialysis who had Bovine artegraft arteriovenous fistulas was reviewed. The indications for operation, the standard technique, the results, the complications, and the future plans for study of the physiology and hemodynamics of this type of fistula are described. The Bovine artegraft, AV fistula can be created successfully in a high percentage of patients, offers ease of needle placement, and effective dialysis with very little complication.", "PMID": 961041}
{"id": "pubmed23n0032_4165", "title": "[Guar II. Feeding Trials with Animals].", "content": "Feeding trials with guarbeans or its protein- or carbohdrat-rich fractions with cattle, poultry and rats are briefly summarized. The protein fraction of the bean contains toxic substances of unknown structure. The toxic effect is diminished by heating. In case of addition of the carbohydrate-rich fractions of the bean in the feeding experiments depression of growth is observed probably in connection with the bad utilization of the galactomannan or the decrease of fodder consumption due to the expanding volume of the fodder in the gut.", "contents": "[Guar II. Feeding Trials with Animals]. Feeding trials with guarbeans or its protein- or carbohdrat-rich fractions with cattle, poultry and rats are briefly summarized. The protein fraction of the bean contains toxic substances of unknown structure. The toxic effect is diminished by heating. In case of addition of the carbohydrate-rich fractions of the bean in the feeding experiments depression of growth is observed probably in connection with the bad utilization of the galactomannan or the decrease of fodder consumption due to the expanding volume of the fodder in the gut.", "PMID": 961062}
{"id": "pubmed23n0032_4166", "title": "[Isolation of the Derivatives from Coca-Powder Fumigated by Ethylene Oxide 1,2-14 C and their Structure Suggested on the Basis of I. R. and Mass-Spectrometry].", "content": "In Coca-powder fumigated with Ethylene Oxide-1,2(14)C, several derivatives were isolated by extraction and preparative Thin Layer Chromatography. Of the two compounds isolated from the water-extract, the structures have been suggested as N,N-Bis-(Di-Ethoxy-O-Hydroxy-ethyl)-Isoleucyl-Alanyl-Cysteine (MW = 569)and N-Ethoxy-O-Hydroxyethyl)-Tyrosine (MW = 269), based on I.R. and Mass Spectrometry. Their approximate concentrations were found to be 20 and 50 mg/kg respectively.", "contents": "[Isolation of the Derivatives from Coca-Powder Fumigated by Ethylene Oxide 1,2-14 C and their Structure Suggested on the Basis of I. R. and Mass-Spectrometry]. In Coca-powder fumigated with Ethylene Oxide-1,2(14)C, several derivatives were isolated by extraction and preparative Thin Layer Chromatography. Of the two compounds isolated from the water-extract, the structures have been suggested as N,N-Bis-(Di-Ethoxy-O-Hydroxy-ethyl)-Isoleucyl-Alanyl-Cysteine (MW = 569)and N-Ethoxy-O-Hydroxyethyl)-Tyrosine (MW = 269), based on I.R. and Mass Spectrometry. Their approximate concentrations were found to be 20 and 50 mg/kg respectively.", "PMID": 961063}
{"id": "pubmed23n0032_4167", "title": "[Differentation and timeinterval for detection of tissue cells on a weapon used in a crime (author's transl)].", "content": "There are numerous methods of examining  the weapon used in a crime to detect evidence of blood or of blood-group properties. Forensic literature also contains references to the tracing of organic tissue. In the case against Jaccoud the two medical experts called upon, UNDRITZ and HEGG, found evidence of cells from internal organs on a knife which had possibly been used to commit the crime and which had been stored away for some considerable time. Practical experience has shown that the likelihood of finding cells on the weapon used is small; however, it is greater in the case of abdominal injuries than in the case of stab wounds in the chest. Tests carried out on corpses have revealed evidence that cells from the liver can adhere to a blade plunged into the organ. This happens mostly when a knife with a serrated or wavy edge is used. The abdominal wall has a greater cleaning effect on a blade than clothing does. It is of crucial importance to clarify the environmental conditions to which an object bearing certain traces has been exposed. Over a long period of time it is much easier to detect parts of organs if they have been subjected to swift dehydration (by the wind, for example). A location which is either warm or damp and humid makes identification, even after a few hours, no longer possible. The presence of a single tissue cell, especially if it should have managed to retain its structure after several months' dessication, can never suffice for an organic diagnosis. How many determining characteristics then must be traceable? And how great is the certainty of identification with regard to cells from other organs or to nun-human cell structures - all of which are also subject to environmental and time factors? How many single tissue cells must be identified to determine conclusively that the cells in question came, for example from the liver?", "contents": "[Differentation and timeinterval for detection of tissue cells on a weapon used in a crime (author's transl)]. There are numerous methods of examining  the weapon used in a crime to detect evidence of blood or of blood-group properties. Forensic literature also contains references to the tracing of organic tissue. In the case against Jaccoud the two medical experts called upon, UNDRITZ and HEGG, found evidence of cells from internal organs on a knife which had possibly been used to commit the crime and which had been stored away for some considerable time. Practical experience has shown that the likelihood of finding cells on the weapon used is small; however, it is greater in the case of abdominal injuries than in the case of stab wounds in the chest. Tests carried out on corpses have revealed evidence that cells from the liver can adhere to a blade plunged into the organ. This happens mostly when a knife with a serrated or wavy edge is used. The abdominal wall has a greater cleaning effect on a blade than clothing does. It is of crucial importance to clarify the environmental conditions to which an object bearing certain traces has been exposed. Over a long period of time it is much easier to detect parts of organs if they have been subjected to swift dehydration (by the wind, for example). A location which is either warm or damp and humid makes identification, even after a few hours, no longer possible. The presence of a single tissue cell, especially if it should have managed to retain its structure after several months' dessication, can never suffice for an organic diagnosis. How many determining characteristics then must be traceable? And how great is the certainty of identification with regard to cells from other organs or to nun-human cell structures - all of which are also subject to environmental and time factors? How many single tissue cells must be identified to determine conclusively that the cells in question came, for example from the liver?", "PMID": 961064}
{"id": "pubmed23n0032_4168", "title": "Serum glucose, serum free fatty acids and adipose tissue lipids after fatal hypothermia of cold acclimatized, reserpine or propranolol treated guinea-pigs.", "content": "Surviving ability in frost (-20 degree C) was studied in cold acclimatized guinea-pigs given either reserpine, propranolol or saline. Survival time, rectal temperature at death, serum glucose, serum FFA and triglycerides in the interscapular adipose tissue were determined. Rectal temperature was highest in the reserpine group, in the same animals that endured the frost the shortest time. The survival time had decreased by about a half of that in the controls. Propranolol treatment decreased the living time only slightly. The fact that serum glucose remained high in the reserpine treated animals was obviously related to the short survival time. In the propranolol group glucose values were somewhat higher than in the control group (saline-animals). Reserpine seemed to have inhibited the release of FFA in the warm-acclimatized animals as interpreted from the low serum values. On the other hand, FFA were rather high in the cold-acclimatized reserpine animals. The blocking effect of reserpine reflected also in the higher contents of triglycerides in the adipose tissue both in cold-acclimatized and warm-acclimatized animals. Propranolol prevented slightly the depletion of the triglycerides. Amount of total lipids in the adipose tissue was lower in the cold-acclimatized animals than in the warm-acclimatized ones because of the change of the type of the adipocytes from unilocular to multilocular. The results corroborated the importance of FFA for longer survival in severe cold. Sensitization to reserpine seems to develop during cold-acclimatization. It calls attention to a possible hazard of reserpine treatment in cold environment.", "contents": "Serum glucose, serum free fatty acids and adipose tissue lipids after fatal hypothermia of cold acclimatized, reserpine or propranolol treated guinea-pigs. Surviving ability in frost (-20 degree C) was studied in cold acclimatized guinea-pigs given either reserpine, propranolol or saline. Survival time, rectal temperature at death, serum glucose, serum FFA and triglycerides in the interscapular adipose tissue were determined. Rectal temperature was highest in the reserpine group, in the same animals that endured the frost the shortest time. The survival time had decreased by about a half of that in the controls. Propranolol treatment decreased the living time only slightly. The fact that serum glucose remained high in the reserpine treated animals was obviously related to the short survival time. In the propranolol group glucose values were somewhat higher than in the control group (saline-animals). Reserpine seemed to have inhibited the release of FFA in the warm-acclimatized animals as interpreted from the low serum values. On the other hand, FFA were rather high in the cold-acclimatized reserpine animals. The blocking effect of reserpine reflected also in the higher contents of triglycerides in the adipose tissue both in cold-acclimatized and warm-acclimatized animals. Propranolol prevented slightly the depletion of the triglycerides. Amount of total lipids in the adipose tissue was lower in the cold-acclimatized animals than in the warm-acclimatized ones because of the change of the type of the adipocytes from unilocular to multilocular. The results corroborated the importance of FFA for longer survival in severe cold. Sensitization to reserpine seems to develop during cold-acclimatization. It calls attention to a possible hazard of reserpine treatment in cold environment.", "PMID": 961065}
{"id": "pubmed23n0032_4169", "title": "[Some new aspects in the study of human cremations (author's transl)].", "content": "Thermally induced reactions of bone mineral are outlined. The shrinkage of cremated bones is due to sintering of bone mineral. In an electron microscopic study changes of bone structure caused by thermal treatment and sintered mineral units are described. The problems of estimating the shrinkage factor and the use of cremation weight for identification and discussed. There is a significant difference between mean weights of cremations of senile males and females. The absolute difference indicates, that cremation weight is not a useful criterion for identification. Even in cremated bones, cuts and saw marks can be detected.", "contents": "[Some new aspects in the study of human cremations (author's transl)]. Thermally induced reactions of bone mineral are outlined. The shrinkage of cremated bones is due to sintering of bone mineral. In an electron microscopic study changes of bone structure caused by thermal treatment and sintered mineral units are described. The problems of estimating the shrinkage factor and the use of cremation weight for identification and discussed. There is a significant difference between mean weights of cremations of senile males and females. The absolute difference indicates, that cremation weight is not a useful criterion for identification. Even in cremated bones, cuts and saw marks can be detected.", "PMID": 961066}
{"id": "pubmed23n0032_4170", "title": "[The dangerousness of revolvers cal 4 mm, long (author's transl)].", "content": "Different models of revolvers (cal 4mm, long), which are obtainable without special permission in Germany, were examinated as to their dangerousness. Both measurements of the kinetic energy of the missiles and investigations on cadavers were performed. Firing of the weapon in its original state yielded kinetic energies of the missiles well below the legal limit of 7,5 Joule. However, even then, fatal injuries of lung and heart could be demonstrated (In one case the os temporale was penetrated). Alteration of the revolver by closing the gas pressure releasing channels with expoxy resin yielded an increase by 45% of the kinetic energy of the missiles. If, in addition, the propelling charge of the ammunition was augmented with some more gun powder the kinetic energy of the missiles rose by 220 %, while such altered ammunition alone caused an increase of 75 %. In every case fatal injuries on head and thorax  could be produced. Based on the results of this investigation proposals to amend the existing laws were made.", "contents": "[The dangerousness of revolvers cal 4 mm, long (author's transl)]. Different models of revolvers (cal 4mm, long), which are obtainable without special permission in Germany, were examinated as to their dangerousness. Both measurements of the kinetic energy of the missiles and investigations on cadavers were performed. Firing of the weapon in its original state yielded kinetic energies of the missiles well below the legal limit of 7,5 Joule. However, even then, fatal injuries of lung and heart could be demonstrated (In one case the os temporale was penetrated). Alteration of the revolver by closing the gas pressure releasing channels with expoxy resin yielded an increase by 45% of the kinetic energy of the missiles. If, in addition, the propelling charge of the ammunition was augmented with some more gun powder the kinetic energy of the missiles rose by 220 %, while such altered ammunition alone caused an increase of 75 %. In every case fatal injuries on head and thorax  could be produced. Based on the results of this investigation proposals to amend the existing laws were made.", "PMID": 961067}
{"id": "pubmed23n0032_4171", "title": "[About the problem of \"lungs in drowning\" (author's transl)].", "content": "In order to remove any misunderstanding I would like to reply to the publication by HEINEN und DOTZAUER as follows: The statements and conclusions of the authors must inevitably give rise to the impression that my detailed investigations of the reticular fibre texture of the drowned lung is to a large extent non-specific and thus of no use for an expert opinion on death by drowning. The authors, who base their statements on a dissertation by Mrs. HEINEN, have apparently overlooked the differences in aetiology between typical and atypical drowning and thus also have misunderstood the pathogenesis of the typical drowned lung both with respect to gross and fine tissue aspects. Characteristic of typical drowning is acute diffuse bronchostenotic emphysems of the lung.", "contents": "[About the problem of \"lungs in drowning\" (author's transl)]. In order to remove any misunderstanding I would like to reply to the publication by HEINEN und DOTZAUER as follows: The statements and conclusions of the authors must inevitably give rise to the impression that my detailed investigations of the reticular fibre texture of the drowned lung is to a large extent non-specific and thus of no use for an expert opinion on death by drowning. The authors, who base their statements on a dissertation by Mrs. HEINEN, have apparently overlooked the differences in aetiology between typical and atypical drowning and thus also have misunderstood the pathogenesis of the typical drowned lung both with respect to gross and fine tissue aspects. Characteristic of typical drowning is acute diffuse bronchostenotic emphysems of the lung.", "PMID": 961068}
{"id": "pubmed23n0032_4172", "title": "[Alteration of textilfibres by explosion gases expelled distant from the muzzle (author's transl)].", "content": "This paper presents the reconstruction of an unusual case of suicide. After raiding a branch-bank a robber fled shooting with his Sauer-Western revolver caliber .44 magnum at the pursuing policemen and succeeded in wrestling a pistol Walther caliber 7,65 mm from them. Under the fire of sub-machine guns he destroyed himself by a shot to the neck. Our investigations concerned a textile damage at the front of the sweater of the deceased surrounded by primer residue, showing characteristics of a close-up shot. The damage was identified as effect of explosion gases exhausting far-off the muzzle. The distance between this injury and the bullet hole corresponded with the length of the barrel of the Sauer-Western revolver and could be used for identification; it confirmed the diagnosis of a close-up shot at the neck, too. Collateral experiments with shots from distant ranges developed spadiceous melt figures of textile fibers around the bullet hole, the appearance of which is considered proof for a close-up shot commonly.", "contents": "[Alteration of textilfibres by explosion gases expelled distant from the muzzle (author's transl)]. This paper presents the reconstruction of an unusual case of suicide. After raiding a branch-bank a robber fled shooting with his Sauer-Western revolver caliber .44 magnum at the pursuing policemen and succeeded in wrestling a pistol Walther caliber 7,65 mm from them. Under the fire of sub-machine guns he destroyed himself by a shot to the neck. Our investigations concerned a textile damage at the front of the sweater of the deceased surrounded by primer residue, showing characteristics of a close-up shot. The damage was identified as effect of explosion gases exhausting far-off the muzzle. The distance between this injury and the bullet hole corresponded with the length of the barrel of the Sauer-Western revolver and could be used for identification; it confirmed the diagnosis of a close-up shot at the neck, too. Collateral experiments with shots from distant ranges developed spadiceous melt figures of textile fibers around the bullet hole, the appearance of which is considered proof for a close-up shot commonly.", "PMID": 961069}
{"id": "pubmed23n0032_4173", "title": "[Concerning evidence of parathion (E 605) in an exhumed corpse after 21 months (author's transl)].", "content": "In a corpse exhumed after 21 months evidence of E 605 could still be found in the stomach and in the intestinal contents after isolation by steam distillation, extraction with either followed by identification with palladium chloride reagent, as well as by thin layer chromatographicenzymatic detection. A ccording to investigation and analysis, the dose of E 605 administered in the present murder case should have but slightly exceeded the lethal dose.", "contents": "[Concerning evidence of parathion (E 605) in an exhumed corpse after 21 months (author's transl)]. In a corpse exhumed after 21 months evidence of E 605 could still be found in the stomach and in the intestinal contents after isolation by steam distillation, extraction with either followed by identification with palladium chloride reagent, as well as by thin layer chromatographicenzymatic detection. A ccording to investigation and analysis, the dose of E 605 administered in the present murder case should have but slightly exceeded the lethal dose.", "PMID": 961070}
{"id": "pubmed23n0032_4174", "title": "[Serology and genetics of the Duffy-system (author's transl)].", "content": "Experiences in using anti-Fy(a) and anti-Fy(b) in paternity cases are described. Seemingly incompatible mother-child-pairs are due to a silent allele also present in whites. Dosage differences are inconstant and not fit to proof a single or double gene dose. Because of the uncertainty of genetical background the use of both Fy(a) and Fy(b) in paternity cases gives no more information than does Fy(a) alone.", "contents": "[Serology and genetics of the Duffy-system (author's transl)]. Experiences in using anti-Fy(a) and anti-Fy(b) in paternity cases are described. Seemingly incompatible mother-child-pairs are due to a silent allele also present in whites. Dosage differences are inconstant and not fit to proof a single or double gene dose. Because of the uncertainty of genetical background the use of both Fy(a) and Fy(b) in paternity cases gives no more information than does Fy(a) alone.", "PMID": 961071}
{"id": "pubmed23n0032_4175", "title": "[Forensic usefulness of coronarometric studies (author's transl)].", "content": "Authors examine the forensic usefulness and scope of angiometric studies in cases of sudden death that are presumably caused by coronary heart disease (CHD). Hearts of 35 men aged between 40 and 59 years who died of CHD are studied angiographically, morphologically and microscopically. The control group consists of 34 men of the same range of age who died of unnatural causes. Following to a schematic model with special regard to hemodynamic aspects and the degree of the stenosis a stenosis score is made up for each heart and correlated on a diagram with the corresponding heart weight. It appears that the cases of both groups are different areas but are showing a certain degree of overlap. The variation within both groups is discussed. With regard to the CHD group it is presumed that the factors causing death are too variable as to be defined by the deviced methods exclusively. The causal role of factors other than atherosclerosis in the occurrence of CHD is discussed. Authors conclude that the methods examined could be useful in special forensic problems.", "contents": "[Forensic usefulness of coronarometric studies (author's transl)]. Authors examine the forensic usefulness and scope of angiometric studies in cases of sudden death that are presumably caused by coronary heart disease (CHD). Hearts of 35 men aged between 40 and 59 years who died of CHD are studied angiographically, morphologically and microscopically. The control group consists of 34 men of the same range of age who died of unnatural causes. Following to a schematic model with special regard to hemodynamic aspects and the degree of the stenosis a stenosis score is made up for each heart and correlated on a diagram with the corresponding heart weight. It appears that the cases of both groups are different areas but are showing a certain degree of overlap. The variation within both groups is discussed. With regard to the CHD group it is presumed that the factors causing death are too variable as to be defined by the deviced methods exclusively. The causal role of factors other than atherosclerosis in the occurrence of CHD is discussed. Authors conclude that the methods examined could be useful in special forensic problems.", "PMID": 961072}
{"id": "pubmed23n0032_4176", "title": "[Wounding capacity of bullets after ricochet from sand or concrete (author's transl)].", "content": "Investigation is made upon the ricochet of different bullets (full jacketed and soft nosed) from concrete or sand. The deformation resp. disintegration of the bullets depends on the target material, on the design of the bullet and on the striking velocity. The major resulting bullet fragments are classified following their depth of penetration into pinewood, thus giving an approximate measure of their danger to man. The relation between angle of impact, angle of ricochet and the criteria mentioned above is shown in graphs.", "contents": "[Wounding capacity of bullets after ricochet from sand or concrete (author's transl)]. Investigation is made upon the ricochet of different bullets (full jacketed and soft nosed) from concrete or sand. The deformation resp. disintegration of the bullets depends on the target material, on the design of the bullet and on the striking velocity. The major resulting bullet fragments are classified following their depth of penetration into pinewood, thus giving an approximate measure of their danger to man. The relation between angle of impact, angle of ricochet and the criteria mentioned above is shown in graphs.", "PMID": 961073}
{"id": "pubmed23n0032_4177", "title": "[The pneumothorax as complication of continuous artificial ventilation (author's transl)].", "content": "The authors discuss briefly the main lesions produced during intensive therapy in 500 patients who died under treatment in the Intensive Care Unit of Padova during the period Dec. 13, 1971--Sept. 9, 1974. Specifically 36 cases of pneumothorax induced by automatic respiration are analyzed from a clinical and pathological viewpoint.", "contents": "[The pneumothorax as complication of continuous artificial ventilation (author's transl)]. The authors discuss briefly the main lesions produced during intensive therapy in 500 patients who died under treatment in the Intensive Care Unit of Padova during the period Dec. 13, 1971--Sept. 9, 1974. Specifically 36 cases of pneumothorax induced by automatic respiration are analyzed from a clinical and pathological viewpoint.", "PMID": 961074}
{"id": "pubmed23n0032_4178", "title": "[The ratio of the concentrations between the components of a two phase hypnotic in relation to the time of intoxication (author's transl)].", "content": "Two cases of poisoning with a double phase effect hypnotic were investigated in respect to the extent to which concentrations of short and medium acting barbiturates (hexobarbital, cyclobarbital) could be used for estimating the duration of poisoning. The analysis of brain turned out to be especially suitable. Increasing the duration of poisoning the ratio of the concentrations was shifted towards the longer acting component. Blood the liver seem to be less informative. The method might be used to estimate the interval between beginning of the poisoning and death in case of intoxication with different acting barbiturates.", "contents": "[The ratio of the concentrations between the components of a two phase hypnotic in relation to the time of intoxication (author's transl)]. Two cases of poisoning with a double phase effect hypnotic were investigated in respect to the extent to which concentrations of short and medium acting barbiturates (hexobarbital, cyclobarbital) could be used for estimating the duration of poisoning. The analysis of brain turned out to be especially suitable. Increasing the duration of poisoning the ratio of the concentrations was shifted towards the longer acting component. Blood the liver seem to be less informative. The method might be used to estimate the interval between beginning of the poisoning and death in case of intoxication with different acting barbiturates.", "PMID": 961075}
{"id": "pubmed23n0032_4179", "title": "[Deaths resulting from combined action of alcohol and drug intake (author's transl)].", "content": "11 cases of rapid central death following combination of sublethal doses of alcohol and sedativa are presented with case-history, histology, alcohol-concentrations and toxicological findings.", "contents": "[Deaths resulting from combined action of alcohol and drug intake (author's transl)]. 11 cases of rapid central death following combination of sublethal doses of alcohol and sedativa are presented with case-history, histology, alcohol-concentrations and toxicological findings.", "PMID": 961076}
{"id": "pubmed23n0032_4180", "title": "Microscopic comparison of the synovial changes in rheumatoid arthritis and ostroarthritis.", "content": "Whereas the histopathologic picture of the synovial membrane in rheumatoid arthritis varies widely and that in osteoarthritis displays a small range of changes, light- and transmission electron microscopic studies of synovial sections from both disease entities complement each other and assist reciprocally to corroborate the observed changes. Beyond that comparative survey of the ascertained histopathologic features and their correlation with clinical observations disclose that a study of a larger material of specimens permits with some limitations to infer the nature of the joint disease.", "contents": "Microscopic comparison of the synovial changes in rheumatoid arthritis and ostroarthritis. Whereas the histopathologic picture of the synovial membrane in rheumatoid arthritis varies widely and that in osteoarthritis displays a small range of changes, light- and transmission electron microscopic studies of synovial sections from both disease entities complement each other and assist reciprocally to corroborate the observed changes. Beyond that comparative survey of the ascertained histopathologic features and their correlation with clinical observations disclose that a study of a larger material of specimens permits with some limitations to infer the nature of the joint disease.", "PMID": 961077}
{"id": "pubmed23n0032_4181", "title": "[The value of synovial biopsies in the diagnosis of rheumatoid arthritis and in estimating local inflammatory activity].", "content": "Fifty surgically removed synivial membranes from large joints of patients with rheumatoid arthritis were examined histologically from 10 different areas. The diagnostic reliability and the histologically graded basic and actual activity were estimated. All 3 parameters differed considerably within the individual synovial membrane. To assess the diagnostic value of synovial needle biopsies the results were compared with those of simulated biopsy cylinders. The morphological results gained from the biopsy cylinders were much poorer, showing that needle biopsies are limited diagnostic value.", "contents": "[The value of synovial biopsies in the diagnosis of rheumatoid arthritis and in estimating local inflammatory activity]. Fifty surgically removed synivial membranes from large joints of patients with rheumatoid arthritis were examined histologically from 10 different areas. The diagnostic reliability and the histologically graded basic and actual activity were estimated. All 3 parameters differed considerably within the individual synovial membrane. To assess the diagnostic value of synovial needle biopsies the results were compared with those of simulated biopsy cylinders. The morphological results gained from the biopsy cylinders were much poorer, showing that needle biopsies are limited diagnostic value.", "PMID": 961079}
{"id": "pubmed23n0032_4182", "title": "[Elevated C3-proactivator serum levels in patients with chronic polyarthritis].", "content": "The C3-Proactivator (C3-PA) and the C3 and C4 complements were determined by radio-immuno diffusion in the serum of 72 patients with definite or classical rheumatoid arthritis, 51 patients with osteoarthrosis and 42 healthy subjects. C3-PA and C4 levles were significantly higher in the serum of patients with rheumatoid arthritis than in the control group (C3-PA in 72%, C4 in 37% of the RA patients). There was also a significant difference between seropositive und seronegative cases. Elevation of both components was more frequently found in patients with seronegative rheumatoid arthritis than in the seropositive cases. However, the C3-PA serum level is not correlated with the ESR. Also an elevation of  the C3-PA serum level is not specific for rheumatoid arthritis, it is also found in other inflammatory diseases as well as after surgery.", "contents": "[Elevated C3-proactivator serum levels in patients with chronic polyarthritis]. The C3-Proactivator (C3-PA) and the C3 and C4 complements were determined by radio-immuno diffusion in the serum of 72 patients with definite or classical rheumatoid arthritis, 51 patients with osteoarthrosis and 42 healthy subjects. C3-PA and C4 levles were significantly higher in the serum of patients with rheumatoid arthritis than in the control group (C3-PA in 72%, C4 in 37% of the RA patients). There was also a significant difference between seropositive und seronegative cases. Elevation of both components was more frequently found in patients with seronegative rheumatoid arthritis than in the seropositive cases. However, the C3-PA serum level is not correlated with the ESR. Also an elevation of  the C3-PA serum level is not specific for rheumatoid arthritis, it is also found in other inflammatory diseases as well as after surgery.", "PMID": 961078}
{"id": "pubmed23n0032_4183", "title": "[Variability of the clinical picture and the classification of progressive systemic scleroderma].", "content": "Fiftythree patients with progressive systemic sclerosis were studied. Four of them (3 males) had the diffuse form of the disease. The skin manifestation of this clinical picture is characterized by diffuse progression of the cutaneous sclerosis over almost the whole body surface, except for the hands where it eventually may appear late. The prognosis for these patients it especially poor. Fortyfive patients (44 females) had acrosclerosis in the widest sence. Twentyseven of these (\"acrosclerosis stricto sensu\") had cutaneous sclerosis of the hands, face, and often other parts of the body, but not on the abdomen, arms or thighs. The remaining 18 patients had sclerotic alterations on these surfaces also. In this syndrome (which the authors call \"the intermediary syndrome\"), i.e. where the abdomen, arms, and thighs also are affected, certain internal organs and the joints are more involved than in \"acrosclerosis stricto sensu\". With rare exceptions, a symptomatic tetrade (REST-syndrome) occurred in acrosclerosis and all the intermediary syndromes. This consisted of Raynaud's syndrome (R), esophagopathy (E), cutaneous sclerosis (S), and telangectasia (T). Fifty % of the patients in addition had calcinosis (C), either subcutaneous or para-articular. The tetrade \"REST syndrome\" becomes in these cases the pentade \"CREST syndrome\". The addition of calcinosis to the other four phenomena of the REST syndrome does not alter the frequency of internal organ involvement or the prognosis of the disease. The term \"REST syndrome\" and its variant \"CREST syndrome\" should replace the conservative term \"acrosclerosis\" because they add to the purely cutaneous phenomena other characteristic manifestations of the disease. Two patients could neither be classified under the REST syndrome nor the progressive diffuse syndromes. Two other patients had no cutaneous phenomena (\"scleroderma sine scleroderma\").", "contents": "[Variability of the clinical picture and the classification of progressive systemic scleroderma]. Fiftythree patients with progressive systemic sclerosis were studied. Four of them (3 males) had the diffuse form of the disease. The skin manifestation of this clinical picture is characterized by diffuse progression of the cutaneous sclerosis over almost the whole body surface, except for the hands where it eventually may appear late. The prognosis for these patients it especially poor. Fortyfive patients (44 females) had acrosclerosis in the widest sence. Twentyseven of these (\"acrosclerosis stricto sensu\") had cutaneous sclerosis of the hands, face, and often other parts of the body, but not on the abdomen, arms or thighs. The remaining 18 patients had sclerotic alterations on these surfaces also. In this syndrome (which the authors call \"the intermediary syndrome\"), i.e. where the abdomen, arms, and thighs also are affected, certain internal organs and the joints are more involved than in \"acrosclerosis stricto sensu\". With rare exceptions, a symptomatic tetrade (REST-syndrome) occurred in acrosclerosis and all the intermediary syndromes. This consisted of Raynaud's syndrome (R), esophagopathy (E), cutaneous sclerosis (S), and telangectasia (T). Fifty % of the patients in addition had calcinosis (C), either subcutaneous or para-articular. The tetrade \"REST syndrome\" becomes in these cases the pentade \"CREST syndrome\". The addition of calcinosis to the other four phenomena of the REST syndrome does not alter the frequency of internal organ involvement or the prognosis of the disease. The term \"REST syndrome\" and its variant \"CREST syndrome\" should replace the conservative term \"acrosclerosis\" because they add to the purely cutaneous phenomena other characteristic manifestations of the disease. Two patients could neither be classified under the REST syndrome nor the progressive diffuse syndromes. Two other patients had no cutaneous phenomena (\"scleroderma sine scleroderma\").", "PMID": 961080}
{"id": "pubmed23n0032_4184", "title": "[Morphologic studies on rats with adjuvant disease of long duration].", "content": "Morphological studies were performed on Wistar-AF/Han-EMD-SPF-rats with adjuvant disease of 3 tp 14 months duration. Synovitis was observed in the noninjected paw up to the 6th month. While in the central bones resorption and new bone formation had ceased about the 7th month, periostal bone resorption proceeded until the 14the month. Morphological end results of the longlasting disease were pannus formation and fibrous ankylosis of the joints and severe sclerosis of the affected bones. Amyloid deposits were never observed.", "contents": "[Morphologic studies on rats with adjuvant disease of long duration]. Morphological studies were performed on Wistar-AF/Han-EMD-SPF-rats with adjuvant disease of 3 tp 14 months duration. Synovitis was observed in the noninjected paw up to the 6th month. While in the central bones resorption and new bone formation had ceased about the 7th month, periostal bone resorption proceeded until the 14the month. Morphological end results of the longlasting disease were pannus formation and fibrous ankylosis of the joints and severe sclerosis of the affected bones. Amyloid deposits were never observed.", "PMID": 961081}
{"id": "pubmed23n0032_4185", "title": "[Dealing with time in psychoanalysis].", "content": "Psychoanalysis offers two alternatives: Timelessness as well as the division of time into individual sessions. Experience of analytical time by the patient is being demonstrated by examples from analytical practice in such a manner that from it the phase of development of anal-narcissistic problems can be deferred as the dominating one. The discussion of analytical literature arrives at the same result. In digesting the considerations of H. Kobut on narcissism the problem of the limit, in particular of the asolute limit of death is being described as basic threat to narcissism. The attempt is made to defer the evolutionary conditions of some mechanisms of defense against the conception of death from the anal-narcissistic context.", "contents": "[Dealing with time in psychoanalysis]. Psychoanalysis offers two alternatives: Timelessness as well as the division of time into individual sessions. Experience of analytical time by the patient is being demonstrated by examples from analytical practice in such a manner that from it the phase of development of anal-narcissistic problems can be deferred as the dominating one. The discussion of analytical literature arrives at the same result. In digesting the considerations of H. Kobut on narcissism the problem of the limit, in particular of the asolute limit of death is being described as basic threat to narcissism. The attempt is made to defer the evolutionary conditions of some mechanisms of defense against the conception of death from the anal-narcissistic context.", "PMID": 961091}
{"id": "pubmed23n0032_4186", "title": "[Model of a course of psychosomatic medicine and psychotherapy].", "content": "In 1970 new directions for the training of medical students in Germany have been issued. They include for the first time several compulsory lectures and courses on psychological-medicine, which covers: psychosomatic medicine, psychotherapy, psychoanalysis and medical psychology. Our paper puts to dicussion a new teaching model for psychological medicine that proved to be useful and effective many terms. The standards for our \"practical course in psychosomatic medicine and psychotherapy\" have been derived from the post-graduate training courses for psychoanalysis as offered in Germany for the last 25 years by the two psychoanalytical societies of the DGPPT (German Society for Psychotherapy, Psychosomatic Medicine and Deep Psychology).", "contents": "[Model of a course of psychosomatic medicine and psychotherapy]. In 1970 new directions for the training of medical students in Germany have been issued. They include for the first time several compulsory lectures and courses on psychological-medicine, which covers: psychosomatic medicine, psychotherapy, psychoanalysis and medical psychology. Our paper puts to dicussion a new teaching model for psychological medicine that proved to be useful and effective many terms. The standards for our \"practical course in psychosomatic medicine and psychotherapy\" have been derived from the post-graduate training courses for psychoanalysis as offered in Germany for the last 25 years by the two psychoanalytical societies of the DGPPT (German Society for Psychotherapy, Psychosomatic Medicine and Deep Psychology).", "PMID": 961092}
{"id": "pubmed23n0032_4187", "title": "[2 criteria for making differential diagnosis between primarily psychogenic and primarily organic body symptomatology easier].", "content": "Attation has been drawn to two criteria, which have been found to be helpful in the differentialdiagnosis between primary psychogenic and primary organic symptoms. 1. Psychogenesis is doubtful in cooperative patients from whom an initiating conflict-situation cannot be evaluated, if the time of onset does not relate to onset of symptoms, even if the pattern of symptoms seem to correlate with the structure of personality. 2. If a patient is too readily convinced of an exclusively mental source of his problems, one has to be aware of to deal with mechanisms called \"inhibition of bodily threat\" or \"inhibition of death\". These states of mental inhibition may also serve to demonstrate feelings of revenge against the respective individual.", "contents": "[2 criteria for making differential diagnosis between primarily psychogenic and primarily organic body symptomatology easier]. Attation has been drawn to two criteria, which have been found to be helpful in the differentialdiagnosis between primary psychogenic and primary organic symptoms. 1. Psychogenesis is doubtful in cooperative patients from whom an initiating conflict-situation cannot be evaluated, if the time of onset does not relate to onset of symptoms, even if the pattern of symptoms seem to correlate with the structure of personality. 2. If a patient is too readily convinced of an exclusively mental source of his problems, one has to be aware of to deal with mechanisms called \"inhibition of bodily threat\" or \"inhibition of death\". These states of mental inhibition may also serve to demonstrate feelings of revenge against the respective individual.", "PMID": 961093}
{"id": "pubmed23n0032_4188", "title": "[Anorexia nervosa - thoughts on psychogenesis and psychotherapy].", "content": "In this work I tried to classify Anorexia nervosa into two groups, the central group and the reactive group. The central group contains four different subgroups, the immature, the schizoid, the hysterical and the compulsive type. In the first two subgroups the lack of appetite appears primarily at the onset of the disease, and the fear of growing up plays an important role. In the latter two subgroups the lack of appetite comes later on the basis of a conscious or subconscious suppression of the appetite; here prevails a wish to be slender and the disturbance of the aesthetic value orientation. The basis of this syndrome lies in the interpersonal constellation in the family during the childhood. In tests and interviews of the parents of 20 patients was proved, that the fathers have weak and immature character structures, show little interest in the family, they are hypochondriacl, undecisive and not self-confident. On the contrary the mothers are more normative and they endeavour to accept or compensate some parts of the father-role. In the course of these excessive efforts they lose their motherhood and become secondarily dominating grumbling persons. By the lack of the normal father-daugher-relationship, the maturation of sexuality and feminity is disturbed. The method of psychotherapy of those ambivalent, unmotivated patients with ego-splitting is different from that of other neuroses in many points, such as activity, flexibility and educational control on the part of the therapist instead of the psychoanalytic neutrality. In such cases therapists should not be reluctant to act as an object of identification for these patients.", "contents": "[Anorexia nervosa - thoughts on psychogenesis and psychotherapy]. In this work I tried to classify Anorexia nervosa into two groups, the central group and the reactive group. The central group contains four different subgroups, the immature, the schizoid, the hysterical and the compulsive type. In the first two subgroups the lack of appetite appears primarily at the onset of the disease, and the fear of growing up plays an important role. In the latter two subgroups the lack of appetite comes later on the basis of a conscious or subconscious suppression of the appetite; here prevails a wish to be slender and the disturbance of the aesthetic value orientation. The basis of this syndrome lies in the interpersonal constellation in the family during the childhood. In tests and interviews of the parents of 20 patients was proved, that the fathers have weak and immature character structures, show little interest in the family, they are hypochondriacl, undecisive and not self-confident. On the contrary the mothers are more normative and they endeavour to accept or compensate some parts of the father-role. In the course of these excessive efforts they lose their motherhood and become secondarily dominating grumbling persons. By the lack of the normal father-daugher-relationship, the maturation of sexuality and feminity is disturbed. The method of psychotherapy of those ambivalent, unmotivated patients with ego-splitting is different from that of other neuroses in many points, such as activity, flexibility and educational control on the part of the therapist instead of the psychoanalytic neutrality. In such cases therapists should not be reluctant to act as an object of identification for these patients.", "PMID": 961095}
{"id": "pubmed23n0032_4189", "title": "[Psychodynamics of psychovegetative disorders].", "content": "Based on quotations from Freuds writings on the actual neurosis and quotations from Schultz-Henckes writings on neurasthenia and nervousness, the psychodynamics of psychovegetative disturbances are demonstrated through an examplatory case. In a first attempt the symptoms are categorized, a classification is proposed, and the differential diagnosis is reflected. Next a report is given about the special defence-mechanism known as repression, the highly strained ego-ideal, and the importance of the triggering situation in relation to the etiology. Psychodynamic hypothese about psychovegetative disturbances are supported through therapeutic experiences.", "contents": "[Psychodynamics of psychovegetative disorders]. Based on quotations from Freuds writings on the actual neurosis and quotations from Schultz-Henckes writings on neurasthenia and nervousness, the psychodynamics of psychovegetative disturbances are demonstrated through an examplatory case. In a first attempt the symptoms are categorized, a classification is proposed, and the differential diagnosis is reflected. Next a report is given about the special defence-mechanism known as repression, the highly strained ego-ideal, and the importance of the triggering situation in relation to the etiology. Psychodynamic hypothese about psychovegetative disturbances are supported through therapeutic experiences.", "PMID": 961094}
{"id": "pubmed23n0032_4190", "title": "[Remarks on the physician-patient relationship with cancer patients. Prerequisites, function, and goal of so-called Balint groups in an internal-oncological department].", "content": "Drawing on their experience with their own cancer patients and with the Balint Group in the Oncological Department of Z\u00fcrich's University Hospital, the authors describe the special problems arising in the doctor-patient relationship in this field. They show how the diagnosis of cancer can give rise to a feeling of sudden and complete object loss in the patient, thereby confronting the doctor with his own fear of death. The mobilization of archaic defence mechanisms in both the doctor and the patients can lead to an insoluble double-blind situation unless the doctor is able to give up his defence position and thus make it possible for the patient to give up his own fear of death and to accept the nature of his illness. The authors show how the doctor can break through the isolation of the patient in whose body-ego an archaic bad inner-object has been activated by the cancer, and build up good inner objects for him again. In the last chapter Winnicott's idea the \"intermediate area\" is shown to shed a new light on the phenomenon of redenial or belief in immortality.", "contents": "[Remarks on the physician-patient relationship with cancer patients. Prerequisites, function, and goal of so-called Balint groups in an internal-oncological department]. Drawing on their experience with their own cancer patients and with the Balint Group in the Oncological Department of Z\u00fcrich's University Hospital, the authors describe the special problems arising in the doctor-patient relationship in this field. They show how the diagnosis of cancer can give rise to a feeling of sudden and complete object loss in the patient, thereby confronting the doctor with his own fear of death. The mobilization of archaic defence mechanisms in both the doctor and the patients can lead to an insoluble double-blind situation unless the doctor is able to give up his defence position and thus make it possible for the patient to give up his own fear of death and to accept the nature of his illness. The authors show how the doctor can break through the isolation of the patient in whose body-ego an archaic bad inner-object has been activated by the cancer, and build up good inner objects for him again. In the last chapter Winnicott's idea the \"intermediate area\" is shown to shed a new light on the phenomenon of redenial or belief in immortality.", "PMID": 961096}
{"id": "pubmed23n0032_4191", "title": "[\"Counting\" experiments with ravens using \"matching-from-sample\" methods. A contribution on problem-solving behavior in higher aniMALS].", "content": "The research goal is divided into two parts: 1. Detailed analysis of behavior in selection in a 6-choice matching-from-sample task. 2. Discussion of all observed preferences on the basis of the models of Sutherland and Mackintosh and of Miller, Galanter and Pribram. The experiments were run with 4 one-year-old ravens (Corvus corax); two of them were probably 2 males. The design is similar to Koehler's. In the task the correct responses were equally distributed between all stimuli and positions (random system). Although the task (\"counting\") was learned, a lot of relevant preferences for stimuli, positions, patterns etc. show that problem-solving in higher animals can be very complex. It could be shown that only a detailed analysis may clarify animal learning processes.", "contents": "[\"Counting\" experiments with ravens using \"matching-from-sample\" methods. A contribution on problem-solving behavior in higher aniMALS]. The research goal is divided into two parts: 1. Detailed analysis of behavior in selection in a 6-choice matching-from-sample task. 2. Discussion of all observed preferences on the basis of the models of Sutherland and Mackintosh and of Miller, Galanter and Pribram. The experiments were run with 4 one-year-old ravens (Corvus corax); two of them were probably 2 males. The design is similar to Koehler's. In the task the correct responses were equally distributed between all stimuli and positions (random system). Although the task (\"counting\") was learned, a lot of relevant preferences for stimuli, positions, patterns etc. show that problem-solving in higher animals can be very complex. It could be shown that only a detailed analysis may clarify animal learning processes.", "PMID": 961120}
{"id": "pubmed23n0032_4192", "title": "Vocal communication in the pallid bat, Antrozous pallidus.", "content": "The communication sounds of the pallid bat, Antrozous pallidus, wre studied in the laboratory. Adult pallid bats communicate via four main types of sound emissions: directives, squabble notes, irritation buzzes, and FM orientation pulses. Newborn bats emit only isolation calls. These calls evolve into the adult directive by the twentieth day. Ultrasonic orientation pulses do not appear until 7--9 days postnatally. These appear to arise de novo or may be derived from a shortened isolation call. Irritation buzzes and squabble notees are first emitted at one and two weeks of age respectively, without an apparent precursor. Mother bats nurse only their own infants and recognize them on the basis of auditory and olfactory cues. An apparent vocal signature is present in the temporal patterning of frequencies in the isolation call, and this may function in individual recognition.", "contents": "Vocal communication in the pallid bat, Antrozous pallidus. The communication sounds of the pallid bat, Antrozous pallidus, wre studied in the laboratory. Adult pallid bats communicate via four main types of sound emissions: directives, squabble notes, irritation buzzes, and FM orientation pulses. Newborn bats emit only isolation calls. These calls evolve into the adult directive by the twentieth day. Ultrasonic orientation pulses do not appear until 7--9 days postnatally. These appear to arise de novo or may be derived from a shortened isolation call. Irritation buzzes and squabble notees are first emitted at one and two weeks of age respectively, without an apparent precursor. Mother bats nurse only their own infants and recognize them on the basis of auditory and olfactory cues. An apparent vocal signature is present in the temporal patterning of frequencies in the isolation call, and this may function in individual recognition.", "PMID": 961121}
{"id": "pubmed23n0032_4193", "title": "Guinea-pig vocalizations: their structure, causation and function.", "content": "A colony of approximately 150 adult and infant guinea-pigs was studied in order to investigate the structure, causation and function of guinea-pig vocalizations. Behaviour and sound recording were made in a wide variety of contexts and sounds were analysed on the Kay Electric Sonagraph 6061 B. Sonagrams were measured, and on the basis of physical structure alone, 11 call types were distinguished. Behavioural records were examined and it was suggested that 5 functional categories existed. The significance of vocal communication in guinea-pig social behaviour was discussed.", "contents": "Guinea-pig vocalizations: their structure, causation and function. A colony of approximately 150 adult and infant guinea-pigs was studied in order to investigate the structure, causation and function of guinea-pig vocalizations. Behaviour and sound recording were made in a wide variety of contexts and sounds were analysed on the Kay Electric Sonagraph 6061 B. Sonagrams were measured, and on the basis of physical structure alone, 11 call types were distinguished. Behavioural records were examined and it was suggested that 5 functional categories existed. The significance of vocal communication in guinea-pig social behaviour was discussed.", "PMID": 961122}
{"id": "pubmed23n0032_4194", "title": "[Ethology of the Mediterranean blenny Blennius rouxi Cocco 1833].", "content": "The Mediterranean blenny Blennius rouxi has been studied mainly in the Banyuls-sur-Mer region. Data on its behaviour have been obtained by skin diving, SCUBA diving and observations in captivity. At Banyuls-sur-Mer Blennius rouxi lives at a depth of 1 to 42m. As an exception among Mediterranean blennies, Bl. rouxi feeds by grazing off the substrate. Algae, sponges and polychaetes (Sedentaria) are the main components of its food (HEYMER and ZANDER, in press). We could not confirm that the colouration, a white body with a conspicuous dark horizontal band, can be regarded as a signal of cleaning activity in statu nascendi. The male male have a spatial territory in which they occupy haptic holes. The female female lead a vagabond life and actively join the male male in their territories during the breeding season. Head nodding is an agonistic behaviour against other female female and has an attractive significance for spawning-motivated female female. The male male threaten with a widely opened mouth (threat yawning). Our data and observations on the ethology of Bl. rouxi are discussed and compared with those known of Bl. sphinx, Bl. incognitus, and Bl. zvonimiri, its nearest relatives.", "contents": "[Ethology of the Mediterranean blenny Blennius rouxi Cocco 1833]. The Mediterranean blenny Blennius rouxi has been studied mainly in the Banyuls-sur-Mer region. Data on its behaviour have been obtained by skin diving, SCUBA diving and observations in captivity. At Banyuls-sur-Mer Blennius rouxi lives at a depth of 1 to 42m. As an exception among Mediterranean blennies, Bl. rouxi feeds by grazing off the substrate. Algae, sponges and polychaetes (Sedentaria) are the main components of its food (HEYMER and ZANDER, in press). We could not confirm that the colouration, a white body with a conspicuous dark horizontal band, can be regarded as a signal of cleaning activity in statu nascendi. The male male have a spatial territory in which they occupy haptic holes. The female female lead a vagabond life and actively join the male male in their territories during the breeding season. Head nodding is an agonistic behaviour against other female female and has an attractive significance for spawning-motivated female female. The male male threaten with a widely opened mouth (threat yawning). Our data and observations on the ethology of Bl. rouxi are discussed and compared with those known of Bl. sphinx, Bl. incognitus, and Bl. zvonimiri, its nearest relatives.", "PMID": 961123}
{"id": "pubmed23n0032_4195", "title": "Vocalizations of male Euplectes in captivity.", "content": "The dramatic visual displays of the Euplectes- male male have led to their vocalizations being neglected. 6 male male of Euplectes franciscanus and E. after, and 2 E. hordeaceus- male male were kept in aviaries in sound-proof rooms, where vocalizations were recorded during observations of their behaviour, and later analysed on a sound spectrograph. The calls described and illustrated do not represent the complete repertoire of these species. However, the distinctive songs of E. after and E. franciscanus indicate that detailed field studies of vocalization in this genus are required.", "contents": "Vocalizations of male Euplectes in captivity. The dramatic visual displays of the Euplectes- male male have led to their vocalizations being neglected. 6 male male of Euplectes franciscanus and E. after, and 2 E. hordeaceus- male male were kept in aviaries in sound-proof rooms, where vocalizations were recorded during observations of their behaviour, and later analysed on a sound spectrograph. The calls described and illustrated do not represent the complete repertoire of these species. However, the distinctive songs of E. after and E. franciscanus indicate that detailed field studies of vocalization in this genus are required.", "PMID": 961124}
{"id": "pubmed23n0032_4196", "title": "Ranks and relationships in Highland ponies and Highland Cows.", "content": "Recent studies of primates have questioned the importance of dominance hierarchies in groups living under natural conditions. In a herd of Highland ponies and one of Highland cattle grazing under free-range conditions on the Isle of Rhum (Inner Hebrides) well defined hierarchies were present. The provision of food produced a marked increase in the frequency of agonistic interactions but had no effect on the rank systems of the two herds. While rank was clearly important in affecting the distribution of agonistic interactions, it was poorly related to behaviour in non-agonistic situations.", "contents": "Ranks and relationships in Highland ponies and Highland Cows. Recent studies of primates have questioned the importance of dominance hierarchies in groups living under natural conditions. In a herd of Highland ponies and one of Highland cattle grazing under free-range conditions on the Isle of Rhum (Inner Hebrides) well defined hierarchies were present. The provision of food produced a marked increase in the frequency of agonistic interactions but had no effect on the rank systems of the two herds. While rank was clearly important in affecting the distribution of agonistic interactions, it was poorly related to behaviour in non-agonistic situations.", "PMID": 961125}
{"id": "pubmed23n0032_4197", "title": "[Tumour frequency in autopsy cases. VI. Hodgkin's disease (author's transl)].", "content": "The analysis is based on the catalogue of neoplasms notifiable in the German Democratic Republic (ICD-Code Nrs. 140-209, 210.2, 211.3, 211.9, 225, 226.2, 226.3, 253.0, 253.2, 702, 757.2). At the Medical Academy of Erfurt 22155 autopsies (12212 males, 9943 females) of adults (15 years and upwards) were registrated in the period from 1950 to 1966. 128 cases of Hodgkin's disease (0.6% of autopsies and 1.7% of malignant tumours) were observed among them. The cases are distributed among 80 males (0.7% of males) and 48 females (0.5% of females). There is no difference in frequency. Age and sex distribution, frequency and localization of lymph node and organ involvement are presented.", "contents": "[Tumour frequency in autopsy cases. VI. Hodgkin's disease (author's transl)]. The analysis is based on the catalogue of neoplasms notifiable in the German Democratic Republic (ICD-Code Nrs. 140-209, 210.2, 211.3, 211.9, 225, 226.2, 226.3, 253.0, 253.2, 702, 757.2). At the Medical Academy of Erfurt 22155 autopsies (12212 males, 9943 females) of adults (15 years and upwards) were registrated in the period from 1950 to 1966. 128 cases of Hodgkin's disease (0.6% of autopsies and 1.7% of malignant tumours) were observed among them. The cases are distributed among 80 males (0.7% of males) and 48 females (0.5% of females). There is no difference in frequency. Age and sex distribution, frequency and localization of lymph node and organ involvement are presented.", "PMID": 961126}
{"id": "pubmed23n0032_4198", "title": "[The pathomorphology of the arteria primitiva trigemina, together with some critical comments on the pathomorphology of its adjacent structures and the morphological cerebral complications (author's transl)].", "content": "In the light of six self-reported observations an account is given on the pathomorphology and pathohistology of the arteria primitiva trigemina including morphological changes of neighbouring organs and tissue structures as well as of the brain which are directly casused by the anastomosis. Furthermore, our own findings and those described in the pertinent literature are compared. In three of our own cases considerable calcification of the intima and media of the persistent artery have been demonstrated histologically, On the other hand only a slight involvement of the blood vessel from which it originates and of that one into which it flows as well as of the other arteries of the base of the brain has been observed. This suggests to be the result of special flow and pressure relationships of the blood, and interpreted as locus minoris resistentiae. The discrepancy between the frequency of the neuroradiological demonstration and the clinico-neuropathophysiological importance of the anastomosis, on the one hand, and the obtaining of neuropathological autopsy findings, on the other, has been pointed out.", "contents": "[The pathomorphology of the arteria primitiva trigemina, together with some critical comments on the pathomorphology of its adjacent structures and the morphological cerebral complications (author's transl)]. In the light of six self-reported observations an account is given on the pathomorphology and pathohistology of the arteria primitiva trigemina including morphological changes of neighbouring organs and tissue structures as well as of the brain which are directly casused by the anastomosis. Furthermore, our own findings and those described in the pertinent literature are compared. In three of our own cases considerable calcification of the intima and media of the persistent artery have been demonstrated histologically, On the other hand only a slight involvement of the blood vessel from which it originates and of that one into which it flows as well as of the other arteries of the base of the brain has been observed. This suggests to be the result of special flow and pressure relationships of the blood, and interpreted as locus minoris resistentiae. The discrepancy between the frequency of the neuroradiological demonstration and the clinico-neuropathophysiological importance of the anastomosis, on the one hand, and the obtaining of neuropathological autopsy findings, on the other, has been pointed out.", "PMID": 961127}
{"id": "pubmed23n0032_4199", "title": "[Distribution of the chorionic vessels of the human placenta and their arteriovenous crossings (author's transl)].", "content": "We examined 100 selected placentas concerning architecture and distribution of the vessels of the chorionic plate. The eccentric insertion of the umbilical cord as well as the mixed type of distribution of the chorial arteries and equal areas of arterial blood supply were found to be most frequent. In no case the anastomosis of Hyrtl was absent. A marginal and a very eccentric cord were more frequent in combination with a mixed and magistral type of distribution of the vessels that the lesser eccentric and the central type of insertion. There was no significant correlation between insertion of the cord and the infants' birth weight. Arteries pass over the veins when they cross. Serial sections of 15 crossing vessels have been examined, one specimen was reconstructed graphically. When crossed by major arteries the veins bypass below whereas small arterial branches override larger veins not affecting their course. The cross-like vessels are wrapped up in connective tissue and anchored to the chorion plate. There are equivalents in the morphology of the vessels of the chorion and the retina. Both vessel systems are embedded in hollow bodies exposed to an inner pressure, however, sharing a common media, the crossing artery and vein of the retina represent a more intense anatomical unity. The connective mesenchymal frame of the crossing vessels of the choroin plate, the thickened arterial wall-segments and the venous wall-sectors let functional valves suspect as is known from other areas of blood supply.", "contents": "[Distribution of the chorionic vessels of the human placenta and their arteriovenous crossings (author's transl)]. We examined 100 selected placentas concerning architecture and distribution of the vessels of the chorionic plate. The eccentric insertion of the umbilical cord as well as the mixed type of distribution of the chorial arteries and equal areas of arterial blood supply were found to be most frequent. In no case the anastomosis of Hyrtl was absent. A marginal and a very eccentric cord were more frequent in combination with a mixed and magistral type of distribution of the vessels that the lesser eccentric and the central type of insertion. There was no significant correlation between insertion of the cord and the infants' birth weight. Arteries pass over the veins when they cross. Serial sections of 15 crossing vessels have been examined, one specimen was reconstructed graphically. When crossed by major arteries the veins bypass below whereas small arterial branches override larger veins not affecting their course. The cross-like vessels are wrapped up in connective tissue and anchored to the chorion plate. There are equivalents in the morphology of the vessels of the chorion and the retina. Both vessel systems are embedded in hollow bodies exposed to an inner pressure, however, sharing a common media, the crossing artery and vein of the retina represent a more intense anatomical unity. The connective mesenchymal frame of the crossing vessels of the choroin plate, the thickened arterial wall-segments and the venous wall-sectors let functional valves suspect as is known from other areas of blood supply.", "PMID": 961128}
{"id": "pubmed23n0032_4200", "title": "[Comparative morphometric investigations for the determination of the degree of stenosis of the femora artery (author's transl)].", "content": "Planimetry, point-counting-technique and gravimetry were tested for their suitability for determining the degree of stenosis on the same material, the arteria femoralis dextra out of 103 unselected obduction cases. Pilot studies on the circle showed the planimetric method to be most accurate. On transverse sections of the femoral arteries the results of the point-counting-technique deviate significantly stronger from the planimetric test results in comparison with those of the gravimtric method. The point-counting-technique shows the smallest, the gravimetric method the highest time expenditure. Attention is drawn to the necessity of taking into account the plane of the intact intima when determining the degree of stenosis. Also in the case of high-grade stenoses there are frequently no clinically conspicous disturbances of the blood flow.", "contents": "[Comparative morphometric investigations for the determination of the degree of stenosis of the femora artery (author's transl)]. Planimetry, point-counting-technique and gravimetry were tested for their suitability for determining the degree of stenosis on the same material, the arteria femoralis dextra out of 103 unselected obduction cases. Pilot studies on the circle showed the planimetric method to be most accurate. On transverse sections of the femoral arteries the results of the point-counting-technique deviate significantly stronger from the planimetric test results in comparison with those of the gravimtric method. The point-counting-technique shows the smallest, the gravimetric method the highest time expenditure. Attention is drawn to the necessity of taking into account the plane of the intact intima when determining the degree of stenosis. Also in the case of high-grade stenoses there are frequently no clinically conspicous disturbances of the blood flow.", "PMID": 961129}
{"id": "pubmed23n0032_4201", "title": "[The evaluation of classical morphologic patterns in allergic conditions (author's transl)].", "content": "The main classical morphologic features of the allergic diseases comprise fibrinoid necrosis, tissue eosinophilia, vasculitis, capsulitis and trabeculitis in the spleen (exceptionally also in the lymph nodes) and histiocytic granulomatous reaction. The greatest diagnostic value is attributed to capsulitis and trabeculitis in the spleen and further to vasculitis and fibrinoid necrosis. The other morphologic changes, i.e. tissue eosinophilia and histiocytic granuloma are, from the point of view of differential diagnosis, of minor value. The allergic histiocytic reaction in the lymphnodes seldom exhibits a pseudotumorous pattern.", "contents": "[The evaluation of classical morphologic patterns in allergic conditions (author's transl)]. The main classical morphologic features of the allergic diseases comprise fibrinoid necrosis, tissue eosinophilia, vasculitis, capsulitis and trabeculitis in the spleen (exceptionally also in the lymph nodes) and histiocytic granulomatous reaction. The greatest diagnostic value is attributed to capsulitis and trabeculitis in the spleen and further to vasculitis and fibrinoid necrosis. The other morphologic changes, i.e. tissue eosinophilia and histiocytic granuloma are, from the point of view of differential diagnosis, of minor value. The allergic histiocytic reaction in the lymphnodes seldom exhibits a pseudotumorous pattern.", "PMID": 961130}
{"id": "pubmed23n0032_4202", "title": "[Coronary atherosclerosis, coronary thrombosis and myocardial infarction in autopsy cases. 1st communication: Preliminary investigations: atherosclerosis of the aorta and the large arteries (author's transl)].", "content": "28978 autopsy reports of young (after the 14th year of age) and adult patients autopsied during the period from 1.1 1951 until 31. 12 1969 were scored for atherosclerotic changes of the aorta and the large arteries. The informations were transferred to punched cards and processed with electronic data equipment. The methods of the statistical evaluation are described. 52.96 per cent of the cases revealed an aortic atherosclerosis. 17.96 per cent showed an atherosclerosis of the cerebral arteries, 31.16 per cent of the cases had atherosclerotic changes of the kidneys. All groups of diseases showed an increase during the observation period. No sex differences were detected.", "contents": "[Coronary atherosclerosis, coronary thrombosis and myocardial infarction in autopsy cases. 1st communication: Preliminary investigations: atherosclerosis of the aorta and the large arteries (author's transl)]. 28978 autopsy reports of young (after the 14th year of age) and adult patients autopsied during the period from 1.1 1951 until 31. 12 1969 were scored for atherosclerotic changes of the aorta and the large arteries. The informations were transferred to punched cards and processed with electronic data equipment. The methods of the statistical evaluation are described. 52.96 per cent of the cases revealed an aortic atherosclerosis. 17.96 per cent showed an atherosclerosis of the cerebral arteries, 31.16 per cent of the cases had atherosclerotic changes of the kidneys. All groups of diseases showed an increase during the observation period. No sex differences were detected.", "PMID": 961131}
{"id": "pubmed23n0032_4203", "title": "[Clinical experiences with the acute normovalemic hemodilution (author's transl)].", "content": "Acute normavolemic hemodilution is a mean of autotransfusion and allow hepatitis prophylaxis in major surgical procedures which general require homologous blood transfusions. The dilutional drop in blood viscosity is followed by an increased cardiac output, while blood pressure and heart rate remain stable. The CO incerase compensates for the reduced oxygen capacity of the diluted blood. Hemodilution was applied in a total of 88 patients. In 46 cases thorough circulatory and laboratory investigations were performed. While an average of 1785 ml blood was withdrawn and replaced synchronically by plasma substitutes, hematocrit was lowered to 24.8% mean and CO rose from 4.4 to 6.01 l/min. In one half of the patients side reactions were observed that occurred in combination as a syndrome in 8 patients: rise in systemic blood pressure and pulmonary artery pressure, disproportional CO increase, peripheral vasoconstriction, and ST-depression in ECG. The possible pathomechanisms of these side reactions are discussed. A sympathetic adrenergic reaction could be excluded by catecholamine estimation. Hyposia may be assumed to be the more probable reason. Since severe side reactions only occured at hematocrit levels below 26%, the dilution waslimited lately to hct 27%. Patients with coronary heart disease, age greater than 70 years, and anemia less than 12 g% hgb were excepted. In 70% of major surgical procedures, e.g. colonic surgery, homologous blood becomes necessary, in 50% in the amount of 2-4 units. The corresponding blood loss of 1000-2000 ml may be compensated by acute normovolemic hemodilution and autotransfusion. In fact, only 15% of our patients required homologous blood transfusions.", "contents": "[Clinical experiences with the acute normovalemic hemodilution (author's transl)]. Acute normavolemic hemodilution is a mean of autotransfusion and allow hepatitis prophylaxis in major surgical procedures which general require homologous blood transfusions. The dilutional drop in blood viscosity is followed by an increased cardiac output, while blood pressure and heart rate remain stable. The CO incerase compensates for the reduced oxygen capacity of the diluted blood. Hemodilution was applied in a total of 88 patients. In 46 cases thorough circulatory and laboratory investigations were performed. While an average of 1785 ml blood was withdrawn and replaced synchronically by plasma substitutes, hematocrit was lowered to 24.8% mean and CO rose from 4.4 to 6.01 l/min. In one half of the patients side reactions were observed that occurred in combination as a syndrome in 8 patients: rise in systemic blood pressure and pulmonary artery pressure, disproportional CO increase, peripheral vasoconstriction, and ST-depression in ECG. The possible pathomechanisms of these side reactions are discussed. A sympathetic adrenergic reaction could be excluded by catecholamine estimation. Hyposia may be assumed to be the more probable reason. Since severe side reactions only occured at hematocrit levels below 26%, the dilution waslimited lately to hct 27%. Patients with coronary heart disease, age greater than 70 years, and anemia less than 12 g% hgb were excepted. In 70% of major surgical procedures, e.g. colonic surgery, homologous blood becomes necessary, in 50% in the amount of 2-4 units. The corresponding blood loss of 1000-2000 ml may be compensated by acute normovolemic hemodilution and autotransfusion. In fact, only 15% of our patients required homologous blood transfusions.", "PMID": 961142}
{"id": "pubmed23n0032_4204", "title": "[Experiences with acute moderate controlled hemodilution (author's transl)].", "content": "The authors present an own method of hemodilution and experiences gained from 10 patients operated for chronic gastric or duodenal ulcer. The therapuetic results of the method show that it can be applied easely and safely in everyday surgical practice. In the patients treated with it no surgical and anaesthesiological complications could be observed during the operations as well as after them. The autors believe that the method of hemodilution for planned surgical operations will contribute to a more economical use of blood transfusions and to reduction in the occurrence of virus hepatitis.", "contents": "[Experiences with acute moderate controlled hemodilution (author's transl)]. The authors present an own method of hemodilution and experiences gained from 10 patients operated for chronic gastric or duodenal ulcer. The therapuetic results of the method show that it can be applied easely and safely in everyday surgical practice. In the patients treated with it no surgical and anaesthesiological complications could be observed during the operations as well as after them. The autors believe that the method of hemodilution for planned surgical operations will contribute to a more economical use of blood transfusions and to reduction in the occurrence of virus hepatitis.", "PMID": 961143}
{"id": "pubmed23n0032_4205", "title": "[Avoidance of blood transfusion by acute normovolemic hemodilution (author's transl)].", "content": "The authors report on 52 cases of acute normovolemic hemodilution carried out in the course of surgical operations planned in advance in the period from February to September, 1975. In this connection questions of method, indication and contro-indication as well as juridical and economic aspects are dealt with discussion. The advantages of acute normavolemic hemodilution by transfusing the patient's own blood taken from him some days before the operation are particularly stressed.", "contents": "[Avoidance of blood transfusion by acute normovolemic hemodilution (author's transl)]. The authors report on 52 cases of acute normovolemic hemodilution carried out in the course of surgical operations planned in advance in the period from February to September, 1975. In this connection questions of method, indication and contro-indication as well as juridical and economic aspects are dealt with discussion. The advantages of acute normavolemic hemodilution by transfusing the patient's own blood taken from him some days before the operation are particularly stressed.", "PMID": 961144}
{"id": "pubmed23n0032_4206", "title": "[Bacteroides infection in surgical patients (author's transl)].", "content": "More than 80 cases of bacteroides infections are reported. 54 cases of these infections were postoperative complications. Relationships between bacteroides infection and diseases of the colon are significant. Not very impressive local symptoms made the diagnoses of abscesses difficult. In most cases bacteroides are susceptible to chloramphenicol and tetracycline. Patients develop agglutinable antibodies after bacteroides infection.", "contents": "[Bacteroides infection in surgical patients (author's transl)]. More than 80 cases of bacteroides infections are reported. 54 cases of these infections were postoperative complications. Relationships between bacteroides infection and diseases of the colon are significant. Not very impressive local symptoms made the diagnoses of abscesses difficult. In most cases bacteroides are susceptible to chloramphenicol and tetracycline. Patients develop agglutinable antibodies after bacteroides infection.", "PMID": 961145}
{"id": "pubmed23n0032_4207", "title": "[Is there any decline in the frequency of perforative appendicitis? (AUTHOR'S TRANSL)].", "content": "The authors are concluding the letality of appendicitis displays a steadily declining tendency. The rate of perforations observed at their institution corresponds to the average in international surgical literature. They found that letality statistics of appendicitis improved considerably during the last century, whilst perforation percentage has remained practically the same, though the conditions in public health care and possibilities of hospitalization have considerably improved. They expect the number of perforations to decline by establishing the diagnosis even more consistently stage and by performing operations at an early stage, as well.", "contents": "[Is there any decline in the frequency of perforative appendicitis? (AUTHOR'S TRANSL)]. The authors are concluding the letality of appendicitis displays a steadily declining tendency. The rate of perforations observed at their institution corresponds to the average in international surgical literature. They found that letality statistics of appendicitis improved considerably during the last century, whilst perforation percentage has remained practically the same, though the conditions in public health care and possibilities of hospitalization have considerably improved. They expect the number of perforations to decline by establishing the diagnosis even more consistently stage and by performing operations at an early stage, as well.", "PMID": 961146}
{"id": "pubmed23n0032_4208", "title": "[Yersinia enterocolitica in acute appendicitis, lymphadenitis mesenterialis and regional enteritis (author's transl)].", "content": "In 895 patients operated on for acute appendicitis we found Yersinia enterocolitica in 41 cases. Another two cases were detected in postoperative serological examinations. Lymphadenitis mesenterialis was present in 17 cases during operation. Once we detected a regional enteritis. Histological examination of the appendix revealed a purulent inflammation in 21 cases and a phlegmonous-gangrenous inflammation in 4 cases. The other 18 patients suffered from Oxyuriasis, coprostasis or scars of the appendix. For this reason it is impossible to exclude an acute appendicitis in patients with cultural or serological identification of Yersinia enterocolitica.", "contents": "[Yersinia enterocolitica in acute appendicitis, lymphadenitis mesenterialis and regional enteritis (author's transl)]. In 895 patients operated on for acute appendicitis we found Yersinia enterocolitica in 41 cases. Another two cases were detected in postoperative serological examinations. Lymphadenitis mesenterialis was present in 17 cases during operation. Once we detected a regional enteritis. Histological examination of the appendix revealed a purulent inflammation in 21 cases and a phlegmonous-gangrenous inflammation in 4 cases. The other 18 patients suffered from Oxyuriasis, coprostasis or scars of the appendix. For this reason it is impossible to exclude an acute appendicitis in patients with cultural or serological identification of Yersinia enterocolitica.", "PMID": 961157}
{"id": "pubmed23n0032_4209", "title": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. I. Course of pregnancy].", "content": "About complications in delivered pregnancies after spontaneous abortions and after interruptions was reported. Altogether the pregnancies of 782 I-parae and 452 II-parae were analysed. Especially we must expect bleeding in pregnancy after interruption. Farther on the cervical insufficiency appears more frequently.", "contents": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. I. Course of pregnancy]. About complications in delivered pregnancies after spontaneous abortions and after interruptions was reported. Altogether the pregnancies of 782 I-parae and 452 II-parae were analysed. Especially we must expect bleeding in pregnancy after interruption. Farther on the cervical insufficiency appears more frequently.", "PMID": 961163}
{"id": "pubmed23n0032_4210", "title": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. II. Course of labor].", "content": "In continuation of information about complications in pregnancy the complications of first and second stage of labour were analysed. Preceded abortions and interruptions do not influence the duration of first both stages. Against that we found an increase of rupture of amniotic membrane and laceration of cervix in birth after interruption. The frequency of premature birth is extraordinary increases at I-parae (21%) and II-parae (15%) after interruption. The Apgar-score showed lower values. The causes of these complications were discussed.", "contents": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. II. Course of labor]. In continuation of information about complications in pregnancy the complications of first and second stage of labour were analysed. Preceded abortions and interruptions do not influence the duration of first both stages. Against that we found an increase of rupture of amniotic membrane and laceration of cervix in birth after interruption. The frequency of premature birth is extraordinary increases at I-parae (21%) and II-parae (15%) after interruption. The Apgar-score showed lower values. The causes of these complications were discussed.", "PMID": 961164}
{"id": "pubmed23n0032_4211", "title": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. III. After-labor-period and puerperium].", "content": "In the third stage of labour we must expect an increase of blood lost and placental retentions after preceded interruption. An accumulation of manual detaching of placenta we ascertained for preceded abortion only. In the childbed we found an certained increase of disturbances in uterine involution. We think, that some consequences for medical care in pregnancies, following abortion and interruption, are very important.", "contents": "[Influence of abortions and interruptions of pregnancies on subsequent deliveries. III. After-labor-period and puerperium]. In the third stage of labour we must expect an increase of blood lost and placental retentions after preceded interruption. An accumulation of manual detaching of placenta we ascertained for preceded abortion only. In the childbed we found an certained increase of disturbances in uterine involution. We think, that some consequences for medical care in pregnancies, following abortion and interruption, are very important.", "PMID": 961165}
{"id": "pubmed23n0032_4212", "title": "[Hormonal contraception--throboembolism and surgical risk].", "content": "Epidemiological, coagulational, haemodynamical, and morphological studies are critically analysed in this review. Our attitude about the surgical operation risk under hormonal contraceptives is explained. In smaller operative measures there musn't be a break in hormonal contraception. Planned greater operations are to performe only 4 to 6 weeks after cessation of hormonal contraceptives, i.e., after the first spontaneous menstrual bleeding. Hints for the necessity greater operations without a removal after pill or an additional risk factors are given.", "contents": "[Hormonal contraception--throboembolism and surgical risk]. Epidemiological, coagulational, haemodynamical, and morphological studies are critically analysed in this review. Our attitude about the surgical operation risk under hormonal contraceptives is explained. In smaller operative measures there musn't be a break in hormonal contraception. Planned greater operations are to performe only 4 to 6 weeks after cessation of hormonal contraceptives, i.e., after the first spontaneous menstrual bleeding. Hints for the necessity greater operations without a removal after pill or an additional risk factors are given.", "PMID": 961166}
{"id": "pubmed23n0032_4213", "title": "[Changes in ligated blood-vessel stumps during abdominal hysterectomy including the removal of the adnexa uteri].", "content": "Histological changes of ligated blood vessels were studied on 30 women of 41 to 57 years who had been selected according to certain criteria. For this purpose we usually resected the ligg. suspensoria ovarii, parts of the mesosalpinx, lig. latum and rotundum, the vasa uterina, segments of the basale parametrium the plexus vesico-vaginalis and others after 15 to 25 minutes. The abdominal hysterectomy was in most cases indicated due to uterus myomatosus. The histological examinations showed that, in spite of the ligation of the vessels the organism protects itself by forming a closing thrombus preventing a hemorrhage threatening life as well as a secondary hemorrhage at a later stage due to demarcation of the distal stump end. The occlusion consist of merged thrombocytes, coagulated fibrin and other blood elements; the lesion of the endothelial cells isvery important. Proximal, to the centre, the intravasal mutations decrease rapidly, the lumen is in most of the cases empty. Only in 16% of the lumina of arteries or arterioles and in 20% of the veins there were no hemostatic plugs found. The problem of hemostasis during the gynecological operation is only being touched upon, possible complications due to circulation and perfusion lesions are mentioned. A pathological extent of the coagulation in small pelvis is likely to be the reason for an embolism.", "contents": "[Changes in ligated blood-vessel stumps during abdominal hysterectomy including the removal of the adnexa uteri]. Histological changes of ligated blood vessels were studied on 30 women of 41 to 57 years who had been selected according to certain criteria. For this purpose we usually resected the ligg. suspensoria ovarii, parts of the mesosalpinx, lig. latum and rotundum, the vasa uterina, segments of the basale parametrium the plexus vesico-vaginalis and others after 15 to 25 minutes. The abdominal hysterectomy was in most cases indicated due to uterus myomatosus. The histological examinations showed that, in spite of the ligation of the vessels the organism protects itself by forming a closing thrombus preventing a hemorrhage threatening life as well as a secondary hemorrhage at a later stage due to demarcation of the distal stump end. The occlusion consist of merged thrombocytes, coagulated fibrin and other blood elements; the lesion of the endothelial cells isvery important. Proximal, to the centre, the intravasal mutations decrease rapidly, the lumen is in most of the cases empty. Only in 16% of the lumina of arteries or arterioles and in 20% of the veins there were no hemostatic plugs found. The problem of hemostasis during the gynecological operation is only being touched upon, possible complications due to circulation and perfusion lesions are mentioned. A pathological extent of the coagulation in small pelvis is likely to be the reason for an embolism.", "PMID": 961167}
{"id": "pubmed23n0032_4214", "title": "[Morphological problems of chronic placental insufficiency].", "content": "The possible causes of chronic placental insufficiency you can subdivide in maternal, maternal-uterine, placental and, fetale factors. Among the maternal-uterine factors are morphological changes of the myometrial and decidual spiral arteries evident in respect of the uteroplacental exchange function. Further are commented intensively the placental factors of fetal malnutrition. There we will put in the front the disturbances of implantation, of circulation in the maternal and fetal system and of placentation (maturation). We demonstrate two possibilities of quantification in chronic placental insufficiency. That are measures of the uteroplacental contact area as well as the estimation of placental basal membrane thickness. We mean, by both the methods important references for the existence of a chronic placental insufficiency can be given.", "contents": "[Morphological problems of chronic placental insufficiency]. The possible causes of chronic placental insufficiency you can subdivide in maternal, maternal-uterine, placental and, fetale factors. Among the maternal-uterine factors are morphological changes of the myometrial and decidual spiral arteries evident in respect of the uteroplacental exchange function. Further are commented intensively the placental factors of fetal malnutrition. There we will put in the front the disturbances of implantation, of circulation in the maternal and fetal system and of placentation (maturation). We demonstrate two possibilities of quantification in chronic placental insufficiency. That are measures of the uteroplacental contact area as well as the estimation of placental basal membrane thickness. We mean, by both the methods important references for the existence of a chronic placental insufficiency can be given.", "PMID": 961168}
{"id": "pubmed23n0032_4215", "title": "[Histological changes on the wound- and ablatic-areas of the placenta in uterus during the after-labor period].", "content": "30 women were examined to determine the postpartal hemostasis during a section caesarea. Immediately after the ablatio of the placenta the wound is covered with tightly adhering blood clots containing a large amount of fibrin. The fibrin have mostly a lamellar structure, there are mainly erythrocytes incorporated. Fibrin can also be found in the neighbourhood of rare aggregates of bloodplatelets and the plugs. Thus the decidua is being covered in varying degrees of intensity, the lumens of the blood vessels are invisible. The obstruction of the vessels contain large amounts of fibrin, platelet thrombi and, in places, erythrocytes, which indicate a mixed form of thrombi. The intravescular thrombi continuously changes into extravascular coagulation process. Residual decidua and trophoblast cells are also involved in the obturation. There are also other changes of blood vessels evident, such as constrictions, deformations, deposits of fibrin on the walls and parietal thrombi. The role of the fibrin on hemostasis is an important one, whereas the importance of the platelets is rather small. After 5 to 15 minutes there is still more fibrin available which supports the hardness increase and stabilization of hemostasis. The mutations are limited to the bed of the placenta and do not go deep, as deep as the wall of the uterus, where the vessels are usually bloodless and narrow. The hemostasis itself is being induced by the separation of the placenta which happens simultaneously with the activities of the muscular system.", "contents": "[Histological changes on the wound- and ablatic-areas of the placenta in uterus during the after-labor period]. 30 women were examined to determine the postpartal hemostasis during a section caesarea. Immediately after the ablatio of the placenta the wound is covered with tightly adhering blood clots containing a large amount of fibrin. The fibrin have mostly a lamellar structure, there are mainly erythrocytes incorporated. Fibrin can also be found in the neighbourhood of rare aggregates of bloodplatelets and the plugs. Thus the decidua is being covered in varying degrees of intensity, the lumens of the blood vessels are invisible. The obstruction of the vessels contain large amounts of fibrin, platelet thrombi and, in places, erythrocytes, which indicate a mixed form of thrombi. The intravescular thrombi continuously changes into extravascular coagulation process. Residual decidua and trophoblast cells are also involved in the obturation. There are also other changes of blood vessels evident, such as constrictions, deformations, deposits of fibrin on the walls and parietal thrombi. The role of the fibrin on hemostasis is an important one, whereas the importance of the platelets is rather small. After 5 to 15 minutes there is still more fibrin available which supports the hardness increase and stabilization of hemostasis. The mutations are limited to the bed of the placenta and do not go deep, as deep as the wall of the uterus, where the vessels are usually bloodless and narrow. The hemostasis itself is being induced by the separation of the placenta which happens simultaneously with the activities of the muscular system.", "PMID": 961169}
{"id": "pubmed23n0032_4216", "title": "[Chorionic epithelium in the resorption-villi of placentas of hypotrophic term-born infants. Morphology in chronic placental insufficiency].", "content": "The chorionic epithel of 61 placentas of hypotrophic term-born (small for date) with or without the sign of dysmaturity will be compared with the chorionic epithel of 25 placentas of eutrophic term-borns after clinical and anamnestic normal pregnancy or enzym-incubated Cryostat- and semi-thin-slides. Different degrees of predegenerative plasmoditrophoblast by proliferation or persistence high differentiated and degenerated Langhanscells are signs of disturbed regeneration processes in chorionic epithel by chronical fetal malnutrition. This functional- morphological disturbance of villous trophoblast low-weighted newborns will be evaluated as a morphological correlat by chronical insufficiency of placenta. The possible connections with functional capacity of chorionic epithel will be discussed.", "contents": "[Chorionic epithelium in the resorption-villi of placentas of hypotrophic term-born infants. Morphology in chronic placental insufficiency]. The chorionic epithel of 61 placentas of hypotrophic term-born (small for date) with or without the sign of dysmaturity will be compared with the chorionic epithel of 25 placentas of eutrophic term-borns after clinical and anamnestic normal pregnancy or enzym-incubated Cryostat- and semi-thin-slides. Different degrees of predegenerative plasmoditrophoblast by proliferation or persistence high differentiated and degenerated Langhanscells are signs of disturbed regeneration processes in chorionic epithel by chronical fetal malnutrition. This functional- morphological disturbance of villous trophoblast low-weighted newborns will be evaluated as a morphological correlat by chronical insufficiency of placenta. The possible connections with functional capacity of chorionic epithel will be discussed.", "PMID": 961170}
{"id": "pubmed23n0032_4217", "title": "[Pregnancy in adolescents].", "content": "The course of pregnancy to patients aged 16 and under during 1957 to 1961 and 1967 to 1973 is reported. There is an increasing number of pregnancies in these groups of adolescent girls. The frequency of delivery has not changed but the number of abortion has become more important. This fact and the complication by a substantially higher frequency of gestosis and by a tendency to premature delivery causes the pregnancy in young girls as a high risk pregnancy which needs intensive care.", "contents": "[Pregnancy in adolescents]. The course of pregnancy to patients aged 16 and under during 1957 to 1961 and 1967 to 1973 is reported. There is an increasing number of pregnancies in these groups of adolescent girls. The frequency of delivery has not changed but the number of abortion has become more important. This fact and the complication by a substantially higher frequency of gestosis and by a tendency to premature delivery causes the pregnancy in young girls as a high risk pregnancy which needs intensive care.", "PMID": 961171}
{"id": "pubmed23n0032_4218", "title": "[Prevention with antibiotics in cesarean section].", "content": "The investigation of 222 patients with caesarean section showed an increasing bacterial invasion of the amniotic fluid, when in the patients preoperative was found: - clear or green colored (meconic) amniotic fluid and intact membranes, - clear amniotic fluid with ruptured membranes and - green-colored (meconic) amniotic fluid and ruptured membranes. In support to some conceptions from the literature a perioperative prophylaxis with antibiotics is recommended in the groups, which are potentially endangered postoperatively.", "contents": "[Prevention with antibiotics in cesarean section]. The investigation of 222 patients with caesarean section showed an increasing bacterial invasion of the amniotic fluid, when in the patients preoperative was found: - clear or green colored (meconic) amniotic fluid and intact membranes, - clear amniotic fluid with ruptured membranes and - green-colored (meconic) amniotic fluid and ruptured membranes. In support to some conceptions from the literature a perioperative prophylaxis with antibiotics is recommended in the groups, which are potentially endangered postoperatively.", "PMID": 961172}
{"id": "pubmed23n0032_4219", "title": "[Organization of prenatal diagnosis of genetic defects].", "content": "The prenatal detection of genetic defects can make concrete predictions on definite diseases that are to be expected. In the indication catalogue of the prenatal diagnosis from the amniotic fluid the chromosomal anomalies are predominant. The basis and the potential consequences of the prenatal diagnosis are surgical treatments and highly differentiated methods which may have consequences on the fetus and on the pregnant woman. Therefore the structure of a prenatal diagnostics centre must to a great extent observe the \"Nihil nocere\". The most important functions in the prenatal diagnostics are incumbent on the surgical gynaecologist. His duties are the indication and the carrying-out of the surgical treatments and measures for the prenatal diagnostics under consideration of the risks existing. The gynaecologist has either further to care for the pregnancy as a risk pregnancy or at corresponding indication to interrupt it even after the 12th week of pregnancy. On these grounds it is recommended to build up a prenatal diagnostic centre in a gynaecological hospital.", "contents": "[Organization of prenatal diagnosis of genetic defects]. The prenatal detection of genetic defects can make concrete predictions on definite diseases that are to be expected. In the indication catalogue of the prenatal diagnosis from the amniotic fluid the chromosomal anomalies are predominant. The basis and the potential consequences of the prenatal diagnosis are surgical treatments and highly differentiated methods which may have consequences on the fetus and on the pregnant woman. Therefore the structure of a prenatal diagnostics centre must to a great extent observe the \"Nihil nocere\". The most important functions in the prenatal diagnostics are incumbent on the surgical gynaecologist. His duties are the indication and the carrying-out of the surgical treatments and measures for the prenatal diagnostics under consideration of the risks existing. The gynaecologist has either further to care for the pregnancy as a risk pregnancy or at corresponding indication to interrupt it even after the 12th week of pregnancy. On these grounds it is recommended to build up a prenatal diagnostic centre in a gynaecological hospital.", "PMID": 961173}
{"id": "pubmed23n0032_4220", "title": "[Behavior of urinary estriol level before normal labor].", "content": "The levels of oestriol in urine of 30 healthy pregnant women in the last 22 to 25 days of pregnancy were determined and the statistical analysis was performed. It was found that the levels of oestriol in urine were the highest in 19-th and 9-th days before labour. This form of curve of oestriol levels was found to be characteristic for pregnant women before normal labour.", "contents": "[Behavior of urinary estriol level before normal labor]. The levels of oestriol in urine of 30 healthy pregnant women in the last 22 to 25 days of pregnancy were determined and the statistical analysis was performed. It was found that the levels of oestriol in urine were the highest in 19-th and 9-th days before labour. This form of curve of oestriol levels was found to be characteristic for pregnant women before normal labour.", "PMID": 961174}
{"id": "pubmed23n0032_4221", "title": "[Level of estriol in the amniotic fluid before labor].", "content": "The level of oestriol in amniotic fluid was estimated in 17 women in pregnancy and in labour. The correlation between level of oestriol and the time of labour was found. However no influence of foetal sex and weight on the level of oestriol was observed. There was also no correlation between the level of oestriol and placenta weight and the type of employment of these women.", "contents": "[Level of estriol in the amniotic fluid before labor]. The level of oestriol in amniotic fluid was estimated in 17 women in pregnancy and in labour. The correlation between level of oestriol and the time of labour was found. However no influence of foetal sex and weight on the level of oestriol was observed. There was also no correlation between the level of oestriol and placenta weight and the type of employment of these women.", "PMID": 961175}
{"id": "pubmed23n0032_4222", "title": "The lecithin/spingomyelin quotient as determined using  a modified method and human placental lactogen in the amniotic fluid.", "content": "Thirty amniotic fluid samples in late gestational age were analysed for HPL-values and the L/S-ratio. The pregnant women used in this study were healthy except three patients who developed a rh-incompatibility. The amniotic fluid was only obtained by abdominal amniocentesis. We found a decreasing tendency of the HPL-values in late pregnancy. The L/S-ratio increased. A significant correlation of both values could not be observed. In all three cases of rh-incompatibility the decrease of HPL and the increase of the L/S-ratio occurred obviously earlier and steeper. Further studies will prove the significance of these results.", "contents": "The lecithin/spingomyelin quotient as determined using  a modified method and human placental lactogen in the amniotic fluid. Thirty amniotic fluid samples in late gestational age were analysed for HPL-values and the L/S-ratio. The pregnant women used in this study were healthy except three patients who developed a rh-incompatibility. The amniotic fluid was only obtained by abdominal amniocentesis. We found a decreasing tendency of the HPL-values in late pregnancy. The L/S-ratio increased. A significant correlation of both values could not be observed. In all three cases of rh-incompatibility the decrease of HPL and the increase of the L/S-ratio occurred obviously earlier and steeper. Further studies will prove the significance of these results.", "PMID": 961176}
{"id": "pubmed23n0032_4223", "title": "[Pathogenesis of amniotic fluid embolism].", "content": "The reason for existant of amniotic fluid embolism is the disposition, which will be favoured by the overaction of the sympathic system, by age and the number of pregnancy. It happens especially by premature rupture of membrane, pathologic contents in the amniotic fluid, high pressure in the uterus, bad uterine muscel with laceration and with opened endymyometric veins. Pathophysiologic connection between the stages of amniotic fluid embolism are discussed in detail.", "contents": "[Pathogenesis of amniotic fluid embolism]. The reason for existant of amniotic fluid embolism is the disposition, which will be favoured by the overaction of the sympathic system, by age and the number of pregnancy. It happens especially by premature rupture of membrane, pathologic contents in the amniotic fluid, high pressure in the uterus, bad uterine muscel with laceration and with opened endymyometric veins. Pathophysiologic connection between the stages of amniotic fluid embolism are discussed in detail.", "PMID": 961177}
{"id": "pubmed23n0032_4224", "title": "[Prevention of neonatal respiratory distress syndrome through the administration of prednisolone in the fetal period].", "content": "The clinical effectiveness of antenatal prednisolone medication (prednisolone hemisuccinate) for the prophylaxis of idiopathic respiratory distress syndrome (IRDS) was studied in 114 premature infants with a gestational age between 28 and 36 weeks. There was a statistically significant difference (chi2 - test, p less than 0,01) between the frequency of IRDS in this group and in another one consisting of 137 premature babies with a nearly identical distribution of gestational age, whose mothers did not get prednisolone before delivery. It could be shown that the time interval between medical induction and delivery should not exceed 2 weeks, since the effect decreases with time. At present prednisolone medication during the fetal period may serve as useful prophylactic measure for IRDS in cases of premature birth. As there are a lot of contraindication against prednisolone and as we do know very much about side effects, it seems necessary to seek for other, less dangerous inductors.", "contents": "[Prevention of neonatal respiratory distress syndrome through the administration of prednisolone in the fetal period]. The clinical effectiveness of antenatal prednisolone medication (prednisolone hemisuccinate) for the prophylaxis of idiopathic respiratory distress syndrome (IRDS) was studied in 114 premature infants with a gestational age between 28 and 36 weeks. There was a statistically significant difference (chi2 - test, p less than 0,01) between the frequency of IRDS in this group and in another one consisting of 137 premature babies with a nearly identical distribution of gestational age, whose mothers did not get prednisolone before delivery. It could be shown that the time interval between medical induction and delivery should not exceed 2 weeks, since the effect decreases with time. At present prednisolone medication during the fetal period may serve as useful prophylactic measure for IRDS in cases of premature birth. As there are a lot of contraindication against prednisolone and as we do know very much about side effects, it seems necessary to seek for other, less dangerous inductors.", "PMID": 961180}
{"id": "pubmed23n0032_4225", "title": "[Methods of combined antenatal diagnosis of maturity].", "content": "A combined diagnosis of maturity is described, which is harmless in a correct technique. The method can be carried out in each of obstetrical hospital together with a routine laboratory. By means of the combined diagnosis a \"maturity-index\" is performed, which permits a certain assessment of the survival-chance of the praemature delivered children. In connection with the ultrasound-cephalometrie also there is an exact evidence to the duration of pregnancy and the tendence of intrauterine growth of the fetus.", "contents": "[Methods of combined antenatal diagnosis of maturity]. A combined diagnosis of maturity is described, which is harmless in a correct technique. The method can be carried out in each of obstetrical hospital together with a routine laboratory. By means of the combined diagnosis a \"maturity-index\" is performed, which permits a certain assessment of the survival-chance of the praemature delivered children. In connection with the ultrasound-cephalometrie also there is an exact evidence to the duration of pregnancy and the tendence of intrauterine growth of the fetus.", "PMID": 961181}
{"id": "pubmed23n0032_4226", "title": "[Clinical significance of surgeon's residual bradycardia].", "content": "In connection with the observation of new quantitative electronic dates we undertook bradycardia r\u00e9siduelle from Sureau a critical value in account. The investigations result from non-digital method. We didn't find statistical significant coherence between bradycardie r\u00e9siduelle and Apgar-score past 1, 5, 10 and 60 minuts p.p., the pHf and the delta pHf. Also in relation to the neurological investigations of the corresponding children we hadn't statistical significant correlations. The causes for this perception are discussed.", "contents": "[Clinical significance of surgeon's residual bradycardia]. In connection with the observation of new quantitative electronic dates we undertook bradycardia r\u00e9siduelle from Sureau a critical value in account. The investigations result from non-digital method. We didn't find statistical significant coherence between bradycardie r\u00e9siduelle and Apgar-score past 1, 5, 10 and 60 minuts p.p., the pHf and the delta pHf. Also in relation to the neurological investigations of the corresponding children we hadn't statistical significant correlations. The causes for this perception are discussed.", "PMID": 961182}
{"id": "pubmed23n0032_4227", "title": "[Behavior of serum alkaline during pregnancy. II. Pathological pregnancy].", "content": "832 estimations of heat stable alkaline phosphatase (HSAP) and of heat alkaline phosphatase (HLAP) were carried out simultaneously in late pregnant women at 25th to the 42nd weeks of pregnancy. 147 of them delivered children with normal birth-weight. All these women suffered from pre-eclampsia, hypertension or any kind of superimposed pre-eclampsia. 110 other pregnant women with or without symptoms of pre-eclampsia gave birth to small for dates babies. In addition, the values of these patients were compared with 372 estimations of the same enzymes carried out in 120 patients with normal pregnancy and outcome of normal weighted children. The site of the values of every group showed no typical correlation to the course and outcome of their pregnancy. Regarding four special criterions it was possible to give a good prediction by serial determinations for the weight of the newborn in 80 per cent of the cases. A correlation between the urinary excretion of total oestrogens as well as HLAP and the values of HSAP was to be found only in some groups of patients.", "contents": "[Behavior of serum alkaline during pregnancy. II. Pathological pregnancy]. 832 estimations of heat stable alkaline phosphatase (HSAP) and of heat alkaline phosphatase (HLAP) were carried out simultaneously in late pregnant women at 25th to the 42nd weeks of pregnancy. 147 of them delivered children with normal birth-weight. All these women suffered from pre-eclampsia, hypertension or any kind of superimposed pre-eclampsia. 110 other pregnant women with or without symptoms of pre-eclampsia gave birth to small for dates babies. In addition, the values of these patients were compared with 372 estimations of the same enzymes carried out in 120 patients with normal pregnancy and outcome of normal weighted children. The site of the values of every group showed no typical correlation to the course and outcome of their pregnancy. Regarding four special criterions it was possible to give a good prediction by serial determinations for the weight of the newborn in 80 per cent of the cases. A correlation between the urinary excretion of total oestrogens as well as HLAP and the values of HSAP was to be found only in some groups of patients.", "PMID": 961183}
{"id": "pubmed23n0032_4228", "title": "[Epidemiology of cervix carcinoma in pregnancy].", "content": "It is reported about the prevalence-rate of the precancerous and invasive cancers ofthe cervix uteri with 22,450 pregnant women at the age-groups from the 13th to 48th year, which are registrated by help of the cytological and expanded colposcopical examination from 1968 to 1974. There were found 57 heavy dysplasies (= 0,25%; sp = +/- 0,133), 42 carcinomas in situ (0,18%; sp = +/- 0,113) and 12 invasive cancers of the cervix uteri (= 0,053%; sp = +/- 0,061). It seems to be, that the prevalence-rate of the precancerous and cancers of the cervix uteri is the same at pregnant and non-pregnant women in the generative age.", "contents": "[Epidemiology of cervix carcinoma in pregnancy]. It is reported about the prevalence-rate of the precancerous and invasive cancers ofthe cervix uteri with 22,450 pregnant women at the age-groups from the 13th to 48th year, which are registrated by help of the cytological and expanded colposcopical examination from 1968 to 1974. There were found 57 heavy dysplasies (= 0,25%; sp = +/- 0,133), 42 carcinomas in situ (0,18%; sp = +/- 0,113) and 12 invasive cancers of the cervix uteri (= 0,053%; sp = +/- 0,061). It seems to be, that the prevalence-rate of the precancerous and cancers of the cervix uteri is the same at pregnant and non-pregnant women in the generative age.", "PMID": 961184}
{"id": "pubmed23n0032_4229", "title": "[Intrauterine gas gangrene in the 37th week of pregnancy].", "content": "A report is given on an infection of intrauterine gas gangrene of stage 2 according to Dieminger (Tumpania uteri) in the 37th week of pregnancy which turned out well. The clinical progress of gas gangrene is discussed. The abstention from a caesarian section by reason of indication of the child with suspicion of a severe intrauterine infection, the vaginal delivery which was forced subsequently, the prevailingly prophylactic administration of antibiotics, and the largely initiated infusion and transfusion therapy have in a high degree determined the positive end of the disease. The intensive observation of the woman in labor, who at first had only been clinically conspicuous, led to an early recognition of toxic symptoms, so that an aimed intensive therapy could be carried out.", "contents": "[Intrauterine gas gangrene in the 37th week of pregnancy]. A report is given on an infection of intrauterine gas gangrene of stage 2 according to Dieminger (Tumpania uteri) in the 37th week of pregnancy which turned out well. The clinical progress of gas gangrene is discussed. The abstention from a caesarian section by reason of indication of the child with suspicion of a severe intrauterine infection, the vaginal delivery which was forced subsequently, the prevailingly prophylactic administration of antibiotics, and the largely initiated infusion and transfusion therapy have in a high degree determined the positive end of the disease. The intensive observation of the woman in labor, who at first had only been clinically conspicuous, led to an early recognition of toxic symptoms, so that an aimed intensive therapy could be carried out.", "PMID": 961185}
{"id": "pubmed23n0032_4230", "title": "[Analysis of gestationally conditioned morbidity in the German Democratic Republic].", "content": "During the last years the analysis of the perinatal mortality with its casuistic and statistical aspect has gained importance as an instrument in organizing the health protection services for females. For further achievements in the ante-, intra- and postnatal care according to the social and scientific progress and to the needs of the people a sound knowledge of the morbidity structure is of great value. Relating to these points available data sources are analysed regarding their information contents and significance. Under the aspect of their prevalence the most important diseases during pregnancy are presented.", "contents": "[Analysis of gestationally conditioned morbidity in the German Democratic Republic]. During the last years the analysis of the perinatal mortality with its casuistic and statistical aspect has gained importance as an instrument in organizing the health protection services for females. For further achievements in the ante-, intra- and postnatal care according to the social and scientific progress and to the needs of the people a sound knowledge of the morbidity structure is of great value. Relating to these points available data sources are analysed regarding their information contents and significance. Under the aspect of their prevalence the most important diseases during pregnancy are presented.", "PMID": 961186}
{"id": "pubmed23n0032_4231", "title": "[Effect of the protein-free calf-blood-extract (Solcoseryl) on the excretion of estrogens in chronic placental insufficiency].", "content": "In a double blind study the action of Solcoseryl was tested in 31 patients during late pregnancy with chronic placental insufficiency. Under treatment with Solcoseryl a significant increase in urinary estrogen excretion occurred in relation to the placebo-group.", "contents": "[Effect of the protein-free calf-blood-extract (Solcoseryl) on the excretion of estrogens in chronic placental insufficiency]. In a double blind study the action of Solcoseryl was tested in 31 patients during late pregnancy with chronic placental insufficiency. Under treatment with Solcoseryl a significant increase in urinary estrogen excretion occurred in relation to the placebo-group.", "PMID": 961187}
{"id": "pubmed23n0032_4232", "title": "[Diagnosis and therapy of blood coagulation disorders in intrahepatic cholestasis].", "content": "The reduction of prothrombin level below 5% in a patient with intrahepatic cholestasis of pregnancy is reported. The necessity of controlling the Quick level or better factors II, VII, IX and X is discussed. A well-timed Vitamin K therapy in all cases with impaired secretion of bile during pregnancy is recommended.", "contents": "[Diagnosis and therapy of blood coagulation disorders in intrahepatic cholestasis]. The reduction of prothrombin level below 5% in a patient with intrahepatic cholestasis of pregnancy is reported. The necessity of controlling the Quick level or better factors II, VII, IX and X is discussed. A well-timed Vitamin K therapy in all cases with impaired secretion of bile during pregnancy is recommended.", "PMID": 961188}
{"id": "pubmed23n0032_4233", "title": "[Carbohydrate- and lipid-metabolism changes in monosynaptic H-gestosis].", "content": "The parameter of carbohydrate and fat metabolism (FFA, glyzerin and blood sugar) was tested among forty pregnant women with a monosymptomatic H-gestosis in the thirty-eighth and forty-second week of pregnancy. With a clear rise of all tested metabolic parameters in the single control group and the current significance of an excessive increase in the values of women in late pregnancy further correlations could be statistically proved in respect to the combination group of late pregnancy and second degree hypertonus. A definitive statement on the EPH-gestosis would necessitate further experimental tests.", "contents": "[Carbohydrate- and lipid-metabolism changes in monosynaptic H-gestosis]. The parameter of carbohydrate and fat metabolism (FFA, glyzerin and blood sugar) was tested among forty pregnant women with a monosymptomatic H-gestosis in the thirty-eighth and forty-second week of pregnancy. With a clear rise of all tested metabolic parameters in the single control group and the current significance of an excessive increase in the values of women in late pregnancy further correlations could be statistically proved in respect to the combination group of late pregnancy and second degree hypertonus. A definitive statement on the EPH-gestosis would necessitate further experimental tests.", "PMID": 961189}
{"id": "pubmed23n0032_4234", "title": "[Excrement of the meconium and the intra-partum cardiotocogram].", "content": "Of 2233 parturients, 170 cases with evidence of meconium during delivery were examined. We tried to establish the correlation between the appearance of meconium and the occurance of contraction induced FHR-decelerations in continuous intra partum monitoring. In addition we purposed to determine, whether the appearing of meconium during labor can be used as a clinical prognosite sign for fetal outcome. Our data support the assumption that the passage of meconium during any stage of labor only represents a sign of potential fetal distress showing the intra partum records pathology FHF-patterns. The appearance of meconium alone -- diagnosed at the time of the entrance of parturients or in an advanced stage of labor -- demands supplemented diagnostic methods for a better interpreting of an actual or compensated fetal distress.", "contents": "[Excrement of the meconium and the intra-partum cardiotocogram]. Of 2233 parturients, 170 cases with evidence of meconium during delivery were examined. We tried to establish the correlation between the appearance of meconium and the occurance of contraction induced FHR-decelerations in continuous intra partum monitoring. In addition we purposed to determine, whether the appearing of meconium during labor can be used as a clinical prognosite sign for fetal outcome. Our data support the assumption that the passage of meconium during any stage of labor only represents a sign of potential fetal distress showing the intra partum records pathology FHF-patterns. The appearance of meconium alone -- diagnosed at the time of the entrance of parturients or in an advanced stage of labor -- demands supplemented diagnostic methods for a better interpreting of an actual or compensated fetal distress.", "PMID": 961190}
{"id": "pubmed23n0032_4235", "title": "[Combination of cerclage and drug tocolysis in threatened premature labor].", "content": "The paper reports on the combination of cervix cerclage and tocolysis for treating imminent abortus and premature delivery. Up to now 59 patients have undergone treatment by this method. 37 children were delivered in good health at the approximate date of birth as estimated and survived. The number of survivals out of 15 prematures is 10, bringing the total number of survivals for these children whose life is in peril up to 47 (79,6%). The combined treatment of imminent abortus and premature delivery represents a reasonable alternative to other proven methods.", "contents": "[Combination of cerclage and drug tocolysis in threatened premature labor]. The paper reports on the combination of cervix cerclage and tocolysis for treating imminent abortus and premature delivery. Up to now 59 patients have undergone treatment by this method. 37 children were delivered in good health at the approximate date of birth as estimated and survived. The number of survivals out of 15 prematures is 10, bringing the total number of survivals for these children whose life is in peril up to 47 (79,6%). The combined treatment of imminent abortus and premature delivery represents a reasonable alternative to other proven methods.", "PMID": 961191}
{"id": "pubmed23n0032_4236", "title": "[Unilateral fallopian tube twin pregnancy].", "content": "Report of a rare case of unilateral fallopian twin pregnancy in which the fetuses had been arrested in the uterine tube left in place at an earlier operation by reason of ectopic pregnancy.", "contents": "[Unilateral fallopian tube twin pregnancy]. Report of a rare case of unilateral fallopian twin pregnancy in which the fetuses had been arrested in the uterine tube left in place at an earlier operation by reason of ectopic pregnancy.", "PMID": 961192}
{"id": "pubmed23n0032_4237", "title": "[Results of cytological and histological studies on the uterine cervix after long-term intake of hormonal contraceptive agents].", "content": "Cytologic examinations with 880 women having constantly used oral contraceptives from one to eight years show no agglomeration of epithelial atypias if the primary status was clearly in the negative. Among 669 histologically sure atypias of cervix uteri the part of women using oral contraceptives for at least one year is significantly lower in carcinoma in situ as well as in early invasive carcinoma. The differences in dysplasia are not significant. The results are also discussed with regard to the efficiency of permanent control examinations before and during the use of oral contraceptions. The demand of a further intensification of cytodiagnostic is derived from these examinations.", "contents": "[Results of cytological and histological studies on the uterine cervix after long-term intake of hormonal contraceptive agents]. Cytologic examinations with 880 women having constantly used oral contraceptives from one to eight years show no agglomeration of epithelial atypias if the primary status was clearly in the negative. Among 669 histologically sure atypias of cervix uteri the part of women using oral contraceptives for at least one year is significantly lower in carcinoma in situ as well as in early invasive carcinoma. The differences in dysplasia are not significant. The results are also discussed with regard to the efficiency of permanent control examinations before and during the use of oral contraceptions. The demand of a further intensification of cytodiagnostic is derived from these examinations.", "PMID": 961193}
{"id": "pubmed23n0032_4238", "title": "[Diagnosis and classification of pre- and early stages of cervix carcinoma].", "content": "The cytological and histological possibilities for detection of pre- and early stages of cervical carcinoma are shown. The suggestion of nomenclature should improve the understanding between gynecologists and pathologists.", "contents": "[Diagnosis and classification of pre- and early stages of cervix carcinoma]. The cytological and histological possibilities for detection of pre- and early stages of cervical carcinoma are shown. The suggestion of nomenclature should improve the understanding between gynecologists and pathologists.", "PMID": 961194}
{"id": "pubmed23n0032_4239", "title": "[Incidence of hospital treatment in carcinoma in situ in the German Democratic Republic].", "content": "1. In the period 1969 to 1974 the average frequency of in-patient treatment of carcinoma in situ has more than doubled in the GDR according to the results of an analysis of the hospital case records. There is considerable variation between the different countries and districts. 2. Treatment of carcinoma in situ was provided in 95% of all gynaecological in-patient services in the GDR. 3. The application of a uniform nomenclature of carcinoma in situ based on the agreed concept of its characteristics as a defined intermediate stage in the development of a malignant process should become common practice in order to achieve better comparability.", "contents": "[Incidence of hospital treatment in carcinoma in situ in the German Democratic Republic]. 1. In the period 1969 to 1974 the average frequency of in-patient treatment of carcinoma in situ has more than doubled in the GDR according to the results of an analysis of the hospital case records. There is considerable variation between the different countries and districts. 2. Treatment of carcinoma in situ was provided in 95% of all gynaecological in-patient services in the GDR. 3. The application of a uniform nomenclature of carcinoma in situ based on the agreed concept of its characteristics as a defined intermediate stage in the development of a malignant process should become common practice in order to achieve better comparability.", "PMID": 961195}
{"id": "pubmed23n0032_4240", "title": "[Case contribution on ovarian carcinoma in monovular twins].", "content": "There is a report of a case of a bilateral ovarian cancer in a 51-years old woman whose monocygotic twin sister had died of the same carcinoma one year before. In both cases it was partly a solid partly an adenocarcinoma of the ovaries. According to general statistics of carcinoma here it is an accidental event. Developmental aspects allow us to think of a common genetic disposition. The question is whether in case of a carcinoma of the ovaries in a monocygotic twin the adnectomia in the twin sister should be done.", "contents": "[Case contribution on ovarian carcinoma in monovular twins]. There is a report of a case of a bilateral ovarian cancer in a 51-years old woman whose monocygotic twin sister had died of the same carcinoma one year before. In both cases it was partly a solid partly an adenocarcinoma of the ovaries. According to general statistics of carcinoma here it is an accidental event. Developmental aspects allow us to think of a common genetic disposition. The question is whether in case of a carcinoma of the ovaries in a monocygotic twin the adnectomia in the twin sister should be done.", "PMID": 961196}
{"id": "pubmed23n0032_4241", "title": "[Primary, multiple neoplasms in gynecologic carcinomas].", "content": "Among 4358 malignant tumours of the female genital tract during a period of 15 years (1958 to 1972) there were 108 primary multiple cancers, of them 53 as first tumours prior to and 55 as second tumours during or after genital carcinoma. The different localisations are demonstrated by tables. The number of the second tumours occured after carcinoma of the cervix and the corpus uteri are compared with a calculated rate of incidence. A satisfacting agreement of the levels allows the conclusion: Primary multiple carcinoma determined by accident and corresponds to the general expectation of carcinoma.", "contents": "[Primary, multiple neoplasms in gynecologic carcinomas]. Among 4358 malignant tumours of the female genital tract during a period of 15 years (1958 to 1972) there were 108 primary multiple cancers, of them 53 as first tumours prior to and 55 as second tumours during or after genital carcinoma. The different localisations are demonstrated by tables. The number of the second tumours occured after carcinoma of the cervix and the corpus uteri are compared with a calculated rate of incidence. A satisfacting agreement of the levels allows the conclusion: Primary multiple carcinoma determined by accident and corresponds to the general expectation of carcinoma.", "PMID": 961197}
{"id": "pubmed23n0032_4242", "title": "[Genital carcinoma in pregnancy].", "content": "From 1949 to 1974 there had been found and treated 131 malignant genital tumours during pregnancy post partum and post abortum at the Clinic of Obstetrics and Gynaecology of the University of Jena. The 5-year rate of surival of cervical carcinomas was 59,6%. That is no difference to cases discovered outside of pregnancy. It is discussed the possible influence of pregnancy on the growth of cervical cancer.", "contents": "[Genital carcinoma in pregnancy]. From 1949 to 1974 there had been found and treated 131 malignant genital tumours during pregnancy post partum and post abortum at the Clinic of Obstetrics and Gynaecology of the University of Jena. The 5-year rate of surival of cervical carcinomas was 59,6%. That is no difference to cases discovered outside of pregnancy. It is discussed the possible influence of pregnancy on the growth of cervical cancer.", "PMID": 961198}
{"id": "pubmed23n0032_4243", "title": "[Iatrogenic provocation in female genital tuberculosis free of symptoms].", "content": "Clinical symptoms of the disease are: sterility, relapsing, adnexitis, chronic peritonitis or perimetritis, primary or secundary lack of menstruation, even spontaneous abortion with fever and tubal pregnancy. Any kind of physicotherapy causing hyperaemia or congestion, surgery or sexual hormontherapy may enhance local exacerbation even spread of the disease. Congestion may further enhance the growth of bacteria by supplying more oxygen, and the sex hormones are incorporated by them. These circumstances influence the pathogenesis in a particular manner. The iatrogen provocation can prevented by profilactic antituberculous care. Lack of the mentioned prevention results to local progression, exsudat, fistulas, severe adhaesions, which cannot be solved by surgery. The iatrogen damages can only be prevented if gynecologists and phthysiologists cooperate.", "contents": "[Iatrogenic provocation in female genital tuberculosis free of symptoms]. Clinical symptoms of the disease are: sterility, relapsing, adnexitis, chronic peritonitis or perimetritis, primary or secundary lack of menstruation, even spontaneous abortion with fever and tubal pregnancy. Any kind of physicotherapy causing hyperaemia or congestion, surgery or sexual hormontherapy may enhance local exacerbation even spread of the disease. Congestion may further enhance the growth of bacteria by supplying more oxygen, and the sex hormones are incorporated by them. These circumstances influence the pathogenesis in a particular manner. The iatrogen provocation can prevented by profilactic antituberculous care. Lack of the mentioned prevention results to local progression, exsudat, fistulas, severe adhaesions, which cannot be solved by surgery. The iatrogen damages can only be prevented if gynecologists and phthysiologists cooperate.", "PMID": 961199}
{"id": "pubmed23n0032_4244", "title": "[Late recurrence of female genital tuberculosis].", "content": "5 years patient-material: 336 cases (1042 case-sheats) were analysed for the purpose of studying the cases with relapse. 28 recurrence--7,62 per cent--were registered. Relapse is regarded, if the properly treated patient remained well for at least 5 years, without any signs and symptoms and after negative check-ups carried out at regular intervals became actively ill again. If active disease ocurred earlier this was not regarded a relapse but rather the progression of the original disease, which could be explained either by drug-resistance or insufficient conservative treatment.", "contents": "[Late recurrence of female genital tuberculosis]. 5 years patient-material: 336 cases (1042 case-sheats) were analysed for the purpose of studying the cases with relapse. 28 recurrence--7,62 per cent--were registered. Relapse is regarded, if the properly treated patient remained well for at least 5 years, without any signs and symptoms and after negative check-ups carried out at regular intervals became actively ill again. If active disease ocurred earlier this was not regarded a relapse but rather the progression of the original disease, which could be explained either by drug-resistance or insufficient conservative treatment.", "PMID": 961200}
{"id": "pubmed23n0032_4245", "title": "[Total estrogens and estrogen-creatinine ratio in pregnancy].", "content": "The ratio of estrogen excretion in urine (milligrams per 24 h) divided by creatinine (grams per 24 h) was determined in 571 specimens of 307 pregnant women and compared with total estrogen determinations. Use of estrogen-creatinine ratio reduced the daily variations of total estrogen values by one third resulting in a mean curve with less standard deviation.", "contents": "[Total estrogens and estrogen-creatinine ratio in pregnancy]. The ratio of estrogen excretion in urine (milligrams per 24 h) divided by creatinine (grams per 24 h) was determined in 571 specimens of 307 pregnant women and compared with total estrogen determinations. Use of estrogen-creatinine ratio reduced the daily variations of total estrogen values by one third resulting in a mean curve with less standard deviation.", "PMID": 961201}
{"id": "pubmed23n0032_4246", "title": "[Ultramicroscopic studies on the human corpus luteum menstruationis and graviditatis].", "content": "In high active steroid producing cells of the human corpus luteum we can find in the cytoplasm a well developed smooth endoplasmic reticulum in form of smooth tubules and homogenously spread vesicles resulting from an active metabolic process. We also find specific concertinalike folded membrane complexes which too belong to the endoplasmic reticulum. Numerous mainly tubular mitochondria contain dense lipoid inclusions. Their close conection with the endoplasmic vesicles is expression of a direct interaction of both cell organelles in progesterone production. The transport of the intracellular formed steroid hormones to the capillary takes place via locally enlarged intercellular spaces which communicate with the pericapillary space. On the other hand there are pericellular canaliculi which are coated with a membrane. They have no direct connection to the pericapillar space.", "contents": "[Ultramicroscopic studies on the human corpus luteum menstruationis and graviditatis]. In high active steroid producing cells of the human corpus luteum we can find in the cytoplasm a well developed smooth endoplasmic reticulum in form of smooth tubules and homogenously spread vesicles resulting from an active metabolic process. We also find specific concertinalike folded membrane complexes which too belong to the endoplasmic reticulum. Numerous mainly tubular mitochondria contain dense lipoid inclusions. Their close conection with the endoplasmic vesicles is expression of a direct interaction of both cell organelles in progesterone production. The transport of the intracellular formed steroid hormones to the capillary takes place via locally enlarged intercellular spaces which communicate with the pericapillary space. On the other hand there are pericellular canaliculi which are coated with a membrane. They have no direct connection to the pericapillar space.", "PMID": 961202}
{"id": "pubmed23n0032_4247", "title": "[Anticoagulants and fibrinolytics in pregnancy].", "content": "1. Cumarin-derivates are said to be contraindicated during pregnancy. 2. Heparin, and heparinoides certainly took may be given during pregnancy. 3. Streptokinase is recommended--if indicated--also during pregnancy.", "contents": "[Anticoagulants and fibrinolytics in pregnancy]. 1. Cumarin-derivates are said to be contraindicated during pregnancy. 2. Heparin, and heparinoides certainly took may be given during pregnancy. 3. Streptokinase is recommended--if indicated--also during pregnancy.", "PMID": 961203}
{"id": "pubmed23n0032_4248", "title": "[Statistic evaluation of anamnestic and clinical findings in a premature labor collective of 245 cases].", "content": "245 cases of premature birth were compared from the point of view of frequency of aetiologically significant factors with 200 cases of normal birth. The perinatal progress of these premature and normal births was also followed up. It was possible to obtain statistical confirmation of the connection between prematurity and frequent occurrence of some of these parameters. Prominent amongst these are previous abortions and premature births, cervical insufficiency and malformation of the uterus, multiple pregnancies and anomalous foetal position, also placental insufficiency. Increased mortality and morbidity--including perinatal complications--were recorded at the same time. Knowledge of predisposing factors will in many cases make it possible to recognize in good time when pregnancies may be at risk and give adequate supervision and treatment where appropriate.", "contents": "[Statistic evaluation of anamnestic and clinical findings in a premature labor collective of 245 cases]. 245 cases of premature birth were compared from the point of view of frequency of aetiologically significant factors with 200 cases of normal birth. The perinatal progress of these premature and normal births was also followed up. It was possible to obtain statistical confirmation of the connection between prematurity and frequent occurrence of some of these parameters. Prominent amongst these are previous abortions and premature births, cervical insufficiency and malformation of the uterus, multiple pregnancies and anomalous foetal position, also placental insufficiency. Increased mortality and morbidity--including perinatal complications--were recorded at the same time. Knowledge of predisposing factors will in many cases make it possible to recognize in good time when pregnancies may be at risk and give adequate supervision and treatment where appropriate.", "PMID": 961204}
{"id": "pubmed23n0032_4249", "title": "[Significance of umbilical cord anomalies within the frame of perinatal mortality].", "content": "Anomalies of vessels of the umbilical cord (especially the aplasia of one arteria) are besides abnormal placental implantations and pathological alterations of the placental tissue itself of particular importance for the perinatal mortality. In 3 out of 11 cases of perinatal death of the University Clinic of Jena of the years 1971 and 1972 there had been found 3 times anomalies of vessels of the umbilical cord. Each of these 3 cases showed severe malformations. This emphasizes the necessity of checking the presence of the 3 vessels on the cross-section of the umbilical cord in every case of inspecting secundinae.", "contents": "[Significance of umbilical cord anomalies within the frame of perinatal mortality]. Anomalies of vessels of the umbilical cord (especially the aplasia of one arteria) are besides abnormal placental implantations and pathological alterations of the placental tissue itself of particular importance for the perinatal mortality. In 3 out of 11 cases of perinatal death of the University Clinic of Jena of the years 1971 and 1972 there had been found 3 times anomalies of vessels of the umbilical cord. Each of these 3 cases showed severe malformations. This emphasizes the necessity of checking the presence of the 3 vessels on the cross-section of the umbilical cord in every case of inspecting secundinae.", "PMID": 961205}
{"id": "pubmed23n0032_4250", "title": "[Function of thrombocytes during gestation and puerperium as well as their relations to thrombosis. 1. Function of blood platelets in normal pregnancy, during labor and puerperium].", "content": "Platelet functions during late pregnancy and puerperium were observed. It was found pregnancy variations of the number of platelets, platelet spreading, -aggregation, adhesiveness and thrombelastographic investigations. In this observations is not conclude from increase risk developing a thromboembolic disease during pregnancy and puerperium.", "contents": "[Function of thrombocytes during gestation and puerperium as well as their relations to thrombosis. 1. Function of blood platelets in normal pregnancy, during labor and puerperium]. Platelet functions during late pregnancy and puerperium were observed. It was found pregnancy variations of the number of platelets, platelet spreading, -aggregation, adhesiveness and thrombelastographic investigations. In this observations is not conclude from increase risk developing a thromboembolic disease during pregnancy and puerperium.", "PMID": 961206}
{"id": "pubmed23n0032_4251", "title": "[Early diagnosis of uterine rupture after previous cesarean section].", "content": "A new diagnostic measure for the early detection of still uterus rupture before birth and previous cesarian section is reported. With a portio ready for birth, which permits the finger to pass readily, the possibility exists, after stripping of the membranes, to examine digitally the old caesarian scar in respect of a beginning or already existing scar dehiscence. In 112 investigated patients with this method, once a beginning, six times an incomplete and once a complete uterus rupture prior to another partus was seen. In six cases a stenosis was found in the old section region with retention of the foetal head and threatening uterus rupture.", "contents": "[Early diagnosis of uterine rupture after previous cesarean section]. A new diagnostic measure for the early detection of still uterus rupture before birth and previous cesarian section is reported. With a portio ready for birth, which permits the finger to pass readily, the possibility exists, after stripping of the membranes, to examine digitally the old caesarian scar in respect of a beginning or already existing scar dehiscence. In 112 investigated patients with this method, once a beginning, six times an incomplete and once a complete uterus rupture prior to another partus was seen. In six cases a stenosis was found in the old section region with retention of the foetal head and threatening uterus rupture.", "PMID": 961207}
{"id": "pubmed23n0032_4252", "title": "[Diagnosis of Asherman's syndrome (intrauterine synechiae)].", "content": "On the basis of 120 cases, the authors discuss the diagnostic problems of the Asherman's syndrome. They propose the accomplishment of hysterography in all cases when amenorrhea, hypomenorrhea, sterility or infertility develops after curettage during gestation period and puerperium. They describe the technical difficulties of X-ray examination, the appraisal of medicine certificates and the problems of differential diagnosis. The diagnosis of intrauterine synechia is usally based on the roentgen examination, but uterine sounding must also be performed, and these findings must be compared with the symptoms.", "contents": "[Diagnosis of Asherman's syndrome (intrauterine synechiae)]. On the basis of 120 cases, the authors discuss the diagnostic problems of the Asherman's syndrome. They propose the accomplishment of hysterography in all cases when amenorrhea, hypomenorrhea, sterility or infertility develops after curettage during gestation period and puerperium. They describe the technical difficulties of X-ray examination, the appraisal of medicine certificates and the problems of differential diagnosis. The diagnosis of intrauterine synechia is usally based on the roentgen examination, but uterine sounding must also be performed, and these findings must be compared with the symptoms.", "PMID": 961208}
{"id": "pubmed23n0032_4253", "title": "[Effect of immunization of pregnant women with staphylococcal toxoid on the immunologic status of the child].", "content": "A study was made of the effect of immunization of women during pregnancy of the immunological status of puerperants and their neonates, and also on the course of pregnancy in women and on development of a child aged under one year. Elevation of the IgG level in the blood serum, of the IgA -- in the milk of puerperants, and of the IgM -- in the blood serum of neonates, and also a marked increase in the antistaphylolysin titre in the mother and the neonate was revealed as a result of immunization. Clinical obervations showed such immunization to be harmless for the women, the neonate and the child the first year after birth. Immunization had a favourable effect on the state health of the children the first year after birth; this was expressed in a greater, in comparison with other children, resistance against infections diseases.", "contents": "[Effect of immunization of pregnant women with staphylococcal toxoid on the immunologic status of the child]. A study was made of the effect of immunization of women during pregnancy of the immunological status of puerperants and their neonates, and also on the course of pregnancy in women and on development of a child aged under one year. Elevation of the IgG level in the blood serum, of the IgA -- in the milk of puerperants, and of the IgM -- in the blood serum of neonates, and also a marked increase in the antistaphylolysin titre in the mother and the neonate was revealed as a result of immunization. Clinical obervations showed such immunization to be harmless for the women, the neonate and the child the first year after birth. Immunization had a favourable effect on the state health of the children the first year after birth; this was expressed in a greater, in comparison with other children, resistance against infections diseases.", "PMID": 961224}
{"id": "pubmed23n0032_4254", "title": "[Significance of indices of potential epidemiologic valency of natural foci of tick-borne encephalitis and methods of determining them].", "content": "Potential epidemiological valency (PEV) of the natural foci of tick-borne encephalitis was characterized by the quantity of the infected ticks per unit of the area of the focal territory, and also by the section of the pathway of man's passage along this territory, at which encounter of a single viroforic tick is possible. PEV indices reflected the epidemiological intensity of the natural foci of infection both in the mastered and in the poorly developed areas, and therefore could be used for the epidemiological prognosis on tick-borne encephalitis and in epidemiological search of this infection on the newly-developing territories. The extent of PEV reduction as a result of anti-tick treatment of forests endemic by tick-borne encephalitis which as caricides with a prolonged residual action of the tick-vectors offered a possibility of assessing the epidemiological efficacy of the measures carried out the most objectively.", "contents": "[Significance of indices of potential epidemiologic valency of natural foci of tick-borne encephalitis and methods of determining them]. Potential epidemiological valency (PEV) of the natural foci of tick-borne encephalitis was characterized by the quantity of the infected ticks per unit of the area of the focal territory, and also by the section of the pathway of man's passage along this territory, at which encounter of a single viroforic tick is possible. PEV indices reflected the epidemiological intensity of the natural foci of infection both in the mastered and in the poorly developed areas, and therefore could be used for the epidemiological prognosis on tick-borne encephalitis and in epidemiological search of this infection on the newly-developing territories. The extent of PEV reduction as a result of anti-tick treatment of forests endemic by tick-borne encephalitis which as caricides with a prolonged residual action of the tick-vectors offered a possibility of assessing the epidemiological efficacy of the measures carried out the most objectively.", "PMID": 961225}
{"id": "pubmed23n0032_4255", "title": "[Lysogenic clones of wild-type plague bacteria and characteristics of the phages produced by them].", "content": "Comparative population analysis of 3 lysogenic clones of plague bacteria of wild type by lysogenic properties demonstrated that they failed to show any difference from one another by immunity to homolgous and heterologous phages, but differed by the number of cells capable of producing the phage spontaneously. Lysogenic properties were transmitted by heredity both after the ten-fold passage in the presence of a homologous antiphage serum and after a 10-fold colning. Phages produced by the wild lysogenic clones of plague bacteria were capable of provoking lysogenization of bacteria sensitive to it, they were serologically affiliated and differed by the range of action on plaque and pseudotuberculosis bacteria sensitive to it.", "contents": "[Lysogenic clones of wild-type plague bacteria and characteristics of the phages produced by them]. Comparative population analysis of 3 lysogenic clones of plague bacteria of wild type by lysogenic properties demonstrated that they failed to show any difference from one another by immunity to homolgous and heterologous phages, but differed by the number of cells capable of producing the phage spontaneously. Lysogenic properties were transmitted by heredity both after the ten-fold passage in the presence of a homologous antiphage serum and after a 10-fold colning. Phages produced by the wild lysogenic clones of plague bacteria were capable of provoking lysogenization of bacteria sensitive to it, they were serologically affiliated and differed by the range of action on plaque and pseudotuberculosis bacteria sensitive to it.", "PMID": 961226}
{"id": "pubmed23n0032_4256", "title": "[Biologic effect of atypical mycobacteria against a background of BCG vaccination].", "content": "A study was made of the specific sensitivity to tuberculin  in the animals vaccinated with BCG in case of their additional sensitization with various atypical mycobacteria. The mentioned experimental study appeared to be necessary for the purpose of a more proper treatment of the epidemiological date referred to the sensitivity of man to tuberculin and sensitins against the background of mass BCG vaccination. Preliminary BCG vaccination of the animals with their subsequent infection with atypical mycobacteria altered the allergic response to the antigens from the mycobacteria increasing the response reactions not only to tuberculin, but also to sensitins from mycobacteria of the I--III groups by Runyon's classification, closely connected in antigenic respect with mycobacteria tuberculosis. Skin reactions to sensitins from the saprophytic mycobacteria which had in their composition much less common antigens with mycobacteria tuberculosis, remained at the low level. Sensitization with atypical mycobacteria of animals preliminarily vaccinated with BCG failed to cause significant influence on the production of immunity to the subsequent virulent infection with tuberculosis.", "contents": "[Biologic effect of atypical mycobacteria against a background of BCG vaccination]. A study was made of the specific sensitivity to tuberculin  in the animals vaccinated with BCG in case of their additional sensitization with various atypical mycobacteria. The mentioned experimental study appeared to be necessary for the purpose of a more proper treatment of the epidemiological date referred to the sensitivity of man to tuberculin and sensitins against the background of mass BCG vaccination. Preliminary BCG vaccination of the animals with their subsequent infection with atypical mycobacteria altered the allergic response to the antigens from the mycobacteria increasing the response reactions not only to tuberculin, but also to sensitins from mycobacteria of the I--III groups by Runyon's classification, closely connected in antigenic respect with mycobacteria tuberculosis. Skin reactions to sensitins from the saprophytic mycobacteria which had in their composition much less common antigens with mycobacteria tuberculosis, remained at the low level. Sensitization with atypical mycobacteria of animals preliminarily vaccinated with BCG failed to cause significant influence on the production of immunity to the subsequent virulent infection with tuberculosis.", "PMID": 961227}
{"id": "pubmed23n0032_4257", "title": "[Ultrastructure of Cl. oedematiens spores].", "content": "As a result of study of the ultrastructure of Cl. oedematiens, type B it was found that the first signs of spore formation were anlage of the prespore membrane by invagination of the cytoplasmic membrane. The prespore forms later; the cortex forms between the external and the internal layers of the prespore membrane. Internal spore coat forms by joining of the osmiophilic plates, and exosporum forms simultaneously with the spore coat and is localized externally. Mature spores consist of sporoplasm covered with a membrane, light cortex, external and internal spore coat and and interstitial layer between them and the exosporium.", "contents": "[Ultrastructure of Cl. oedematiens spores]. As a result of study of the ultrastructure of Cl. oedematiens, type B it was found that the first signs of spore formation were anlage of the prespore membrane by invagination of the cytoplasmic membrane. The prespore forms later; the cortex forms between the external and the internal layers of the prespore membrane. Internal spore coat forms by joining of the osmiophilic plates, and exosporum forms simultaneously with the spore coat and is localized externally. Mature spores consist of sporoplasm covered with a membrane, light cortex, external and internal spore coat and and interstitial layer between them and the exosporium.", "PMID": 961228}
{"id": "pubmed23n0032_4258", "title": "[Patterns in the body's immune response to administration of El Tor vibrio cholera toxin to experimental animals].", "content": "A study was made of some regularities attending the mechanisms of antibody production in the organism of experimental animals after the administration of El Tor cholera vibrio toxin. As revealed, the indices of the immune response depended on the chosen model, the method of its determination, the method of administration and the amount of the toxin administered. Dynamics of the primary and secondary responses of the immunocompetent cells of mouse spleen was determined. With the action of the El Tor toxin on the lymphoid tissue of mice there were expressed the general biological regularities of the response -- the optimal amount of the antigen-antibody complexes caused activation of the antibody-producing cells; high doses depressed the most reactive immunocompetent Y-cells. Apparently, there existed a genetic determinination of the immune response of the organism to a definite antigen -- the toxin of the El Tor cholera vibrio. It is supposed that it is necessary to choose groups which mostly require cholera vaccination.", "contents": "[Patterns in the body's immune response to administration of El Tor vibrio cholera toxin to experimental animals]. A study was made of some regularities attending the mechanisms of antibody production in the organism of experimental animals after the administration of El Tor cholera vibrio toxin. As revealed, the indices of the immune response depended on the chosen model, the method of its determination, the method of administration and the amount of the toxin administered. Dynamics of the primary and secondary responses of the immunocompetent cells of mouse spleen was determined. With the action of the El Tor toxin on the lymphoid tissue of mice there were expressed the general biological regularities of the response -- the optimal amount of the antigen-antibody complexes caused activation of the antibody-producing cells; high doses depressed the most reactive immunocompetent Y-cells. Apparently, there existed a genetic determinination of the immune response of the organism to a definite antigen -- the toxin of the El Tor cholera vibrio. It is supposed that it is necessary to choose groups which mostly require cholera vaccination.", "PMID": 961236}
{"id": "pubmed23n0032_4259", "title": "[Microflora of the intestine and biliary tracts in patients with viral hepatitis].", "content": "The authors present the results of studying the microflora if the intestine and of the biliary tracts in patients with viral hepatitis; dysbacteriosis was revealed in 53% of the cases. This percentage proved to decrease with clinical recovery. Bile proved to be often contaminated with microbes -- 77% of the samples; this was particularly true in cases with cholestatic variant of the disease and in cases of moderate severity. The fact of prolonged habitation of the microorganisms in the bile system is of importance -- 68% of the patients still had microbial flora in the gall bladder and the biliary tracts by the time of discharge from the hospital.", "contents": "[Microflora of the intestine and biliary tracts in patients with viral hepatitis]. The authors present the results of studying the microflora if the intestine and of the biliary tracts in patients with viral hepatitis; dysbacteriosis was revealed in 53% of the cases. This percentage proved to decrease with clinical recovery. Bile proved to be often contaminated with microbes -- 77% of the samples; this was particularly true in cases with cholestatic variant of the disease and in cases of moderate severity. The fact of prolonged habitation of the microorganisms in the bile system is of importance -- 68% of the patients still had microbial flora in the gall bladder and the biliary tracts by the time of discharge from the hospital.", "PMID": 961237}
{"id": "pubmed23n0032_4260", "title": "[Epidemiologic aspects of using the dysenterin skin test in institutions for pre-school children for the purpose of detecting potential sources of infection].", "content": "A possibility of considerable reduction of dysentery morbidity in creches and kindergartens by isolation of portential sources of infection among the personnel and children revealed as a result of repeated bacteriological examination, positively reacting to dysenterin was demonstrated in two controlled epidemiological trials. The efficacy of this measure became much less when the test and the corresponding bacteriological examination were performed in the personnel alone. As a whole, the high incidence of positive results of the skin-allergic test in practically healthy children and adults, with an extremely rare isolation of shigellae in persons positively reacting to dysenterin, even in their repeated bacteriological examination, pointed to rather limited possibilities of an effective use of this test in antiepidemic practice for detection of potential sources of infection.", "contents": "[Epidemiologic aspects of using the dysenterin skin test in institutions for pre-school children for the purpose of detecting potential sources of infection]. A possibility of considerable reduction of dysentery morbidity in creches and kindergartens by isolation of portential sources of infection among the personnel and children revealed as a result of repeated bacteriological examination, positively reacting to dysenterin was demonstrated in two controlled epidemiological trials. The efficacy of this measure became much less when the test and the corresponding bacteriological examination were performed in the personnel alone. As a whole, the high incidence of positive results of the skin-allergic test in practically healthy children and adults, with an extremely rare isolation of shigellae in persons positively reacting to dysenterin, even in their repeated bacteriological examination, pointed to rather limited possibilities of an effective use of this test in antiepidemic practice for detection of potential sources of infection.", "PMID": 961238}
{"id": "pubmed23n0032_4261", "title": "[Specific polysaccharides of alkali-forming enterobacteria. II. Separation of a hydrolysate of B. faecalis alcaligenes specific polysaccharide on a column with cellulose].", "content": "Paper and gas-liquid chromatography was applied to the study of monosaccharide composition of specific polysaccharide from B. faecalis alcaligenes. Lipophilic sugar of unknown chemical nature was revealed in the composition of this polysaccharide. Conditions for separation of acid hydrolyzate of the specific polysaccharide of this culture in a column with cellulose powder for the purpose of preparative obtaining of the unknown sugar were elaborated.", "contents": "[Specific polysaccharides of alkali-forming enterobacteria. II. Separation of a hydrolysate of B. faecalis alcaligenes specific polysaccharide on a column with cellulose]. Paper and gas-liquid chromatography was applied to the study of monosaccharide composition of specific polysaccharide from B. faecalis alcaligenes. Lipophilic sugar of unknown chemical nature was revealed in the composition of this polysaccharide. Conditions for separation of acid hydrolyzate of the specific polysaccharide of this culture in a column with cellulose powder for the purpose of preparative obtaining of the unknown sugar were elaborated.", "PMID": 961239}
{"id": "pubmed23n0032_4262", "title": "[Change in specific antigen binding by rabbit lymphocytes during immunization with human serum albumin].", "content": "Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.", "contents": "[Change in specific antigen binding by rabbit lymphocytes during immunization with human serum albumin]. Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.", "PMID": 961241}
{"id": "pubmed23n0032_4263", "title": "[Indentification of staphylococci of hospital origin. I. Specific identification of staphylococci].", "content": "In 22 biological tests a study was made of the properties of 1117 strains of staphylococci isolated from patients and medical personnel surgical departments. The significance of each of the tests for species identification of staphylococci was assessed on the basis of correlation of its results with the results of study of 3 main signs characteristic of S. aureus: the presence of coagulase, anaerobic mannite fermentation, and of DNA-ase. Besides the ones pointed out the following could be considered as properties characteristic of S. aureus: flocculus-forming factor, fibrinolysin, hyaluronidase, lysozyme, golden pigment, tellurite-reductase, aerobic fermentation of mannite and tregalose. A standard system of species identification of staphylococci was elaborated; on its basis assessment was made of the diagnostic value of a number of simple systems used in practice for determination of staphylococcus species.", "contents": "[Indentification of staphylococci of hospital origin. I. Specific identification of staphylococci]. In 22 biological tests a study was made of the properties of 1117 strains of staphylococci isolated from patients and medical personnel surgical departments. The significance of each of the tests for species identification of staphylococci was assessed on the basis of correlation of its results with the results of study of 3 main signs characteristic of S. aureus: the presence of coagulase, anaerobic mannite fermentation, and of DNA-ase. Besides the ones pointed out the following could be considered as properties characteristic of S. aureus: flocculus-forming factor, fibrinolysin, hyaluronidase, lysozyme, golden pigment, tellurite-reductase, aerobic fermentation of mannite and tregalose. A standard system of species identification of staphylococci was elaborated; on its basis assessment was made of the diagnostic value of a number of simple systems used in practice for determination of staphylococcus species.", "PMID": 961242}
{"id": "pubmed23n0032_4264", "title": "[Biochemical characteristics of strains from the Citrobacter and Hafnia genera and their laboratory identification].", "content": "A study of biochemical properties of 176 strains of coprocultures isolated from the patients and persons who sustained various acute enteric diseases, and also from contacts and persons examined for prophylaxis showed that they corresponded to the taxonomic definition of the Citrobacter and Hafnia genus. In connection with the similarity by biochemical properties between bacteria of Citrobacter and Salmonella genus and bacteria of Hafnia and Shigella genus it is of expedience in their identification and differentiation carried out in bacteriological laboratories in the diagnosis of enteric infections to use several biochemical tests.", "contents": "[Biochemical characteristics of strains from the Citrobacter and Hafnia genera and their laboratory identification]. A study of biochemical properties of 176 strains of coprocultures isolated from the patients and persons who sustained various acute enteric diseases, and also from contacts and persons examined for prophylaxis showed that they corresponded to the taxonomic definition of the Citrobacter and Hafnia genus. In connection with the similarity by biochemical properties between bacteria of Citrobacter and Salmonella genus and bacteria of Hafnia and Shigella genus it is of expedience in their identification and differentiation carried out in bacteriological laboratories in the diagnosis of enteric infections to use several biochemical tests.", "PMID": 961244}
{"id": "pubmed23n0032_4265", "title": "[Discriminant studies of immunoglobulins in diseases of the liver and biliary tract].", "content": "Discriminant-analytical studies of immunoglobulins G, A and M in 54 persons with a healthy liver and in 476 persons with various diseases of the liver and biliary tracts, and also calculation of correlations between the extent of proliferation of the hepatic mesenchyma and the concentrations of serum immunoglobulin led to the following conclusions: changes in the behaviour of immunoglobulins were specific of the organ and the disease, of limited significance in the diagnosis of diseases of the liver and biliary tracts. Dependence of the quantitative values of immunoglobulins on the activity of hepatic mesenchyma permitted to determine the significance of the indices for the observation over the course of the disease and for its prognosis. However, in the assessment of these changes one should take into consideration the influence produced on the immunoglobulinogram of extrahepatic concomitant disease, and also different individual capacity of the organism to immunoglobulin synthesis.", "contents": "[Discriminant studies of immunoglobulins in diseases of the liver and biliary tract]. Discriminant-analytical studies of immunoglobulins G, A and M in 54 persons with a healthy liver and in 476 persons with various diseases of the liver and biliary tracts, and also calculation of correlations between the extent of proliferation of the hepatic mesenchyma and the concentrations of serum immunoglobulin led to the following conclusions: changes in the behaviour of immunoglobulins were specific of the organ and the disease, of limited significance in the diagnosis of diseases of the liver and biliary tracts. Dependence of the quantitative values of immunoglobulins on the activity of hepatic mesenchyma permitted to determine the significance of the indices for the observation over the course of the disease and for its prognosis. However, in the assessment of these changes one should take into consideration the influence produced on the immunoglobulinogram of extrahepatic concomitant disease, and also different individual capacity of the organism to immunoglobulin synthesis.", "PMID": 961252}
{"id": "pubmed23n0032_4266", "title": "[Use of computers and mathematical methods in epidemiologic work. IV. Methodical approaches to the epidemic process in dysentery using a computer (image recognition theory)].", "content": "The authors present methodical ways of diagnosis of the type of the epidemic process in dysentery with the aid of computers base on the application of the theory of recognition of imapes.", "contents": "[Use of computers and mathematical methods in epidemiologic work. IV. Methodical approaches to the epidemic process in dysentery using a computer (image recognition theory)]. The authors present methodical ways of diagnosis of the type of the epidemic process in dysentery with the aid of computers base on the application of the theory of recognition of imapes.", "PMID": 961256}
{"id": "pubmed23n0032_4267", "title": "[Experimental concentration and purification of cultural antirabies vaccine].", "content": "A study of 6 series of concentrated and purified -- by ultracentrifugation -- dry cultural rabies vaccine inactivated with ultra-voilet rays demonstrated that in case of a 31--316 increase of concentration immunogenicity of the preparation rose 3--13-fold. Purification of the vaccine took place with its concentration. Additional purification of the concentrated vaccine on aluminium oxide led to a partial loss of the viral protein and to reduction of immunogenicity.", "contents": "[Experimental concentration and purification of cultural antirabies vaccine]. A study of 6 series of concentrated and purified -- by ultracentrifugation -- dry cultural rabies vaccine inactivated with ultra-voilet rays demonstrated that in case of a 31--316 increase of concentration immunogenicity of the preparation rose 3--13-fold. Purification of the vaccine took place with its concentration. Additional purification of the concentrated vaccine on aluminium oxide led to a partial loss of the viral protein and to reduction of immunogenicity.", "PMID": 961258}
{"id": "pubmed23n0032_4268", "title": "[Specific stimulation of the lymphocyte blast transformation reaction in patients with rheumatism under conditions of exposure to delagil, mephenaminic acid and amidopyrine].", "content": "Antirheumatic preparations chiefly inhibited (in vitro) the specific microbial stimulation of the reaction of blast transformation of lymphocytes (RBL) in the patients with torpid rheumocarditis caused by addition of streptococcus nucleoproteid into the medium. Specifically stimulated RBL became intensified in healthy donors on addition to the culture medium of amidopyrin and mephenaminic acid; as to delagil, it depressed the specifically stimulated RBL both in healthy donors and in patients.", "contents": "[Specific stimulation of the lymphocyte blast transformation reaction in patients with rheumatism under conditions of exposure to delagil, mephenaminic acid and amidopyrine]. Antirheumatic preparations chiefly inhibited (in vitro) the specific microbial stimulation of the reaction of blast transformation of lymphocytes (RBL) in the patients with torpid rheumocarditis caused by addition of streptococcus nucleoproteid into the medium. Specifically stimulated RBL became intensified in healthy donors on addition to the culture medium of amidopyrin and mephenaminic acid; as to delagil, it depressed the specifically stimulated RBL both in healthy donors and in patients.", "PMID": 961259}
{"id": "pubmed23n0032_4269", "title": "[Comparative characteristics of the cytotoxic activity of antibrain and different antilymphocyte sera in vitro].", "content": "The cytotoxic test was used to assess the specificity of the action of antisera obtained by immunization of rabbits with the tissue of mouse brain, mouse intact lymphoid cells of various origin, and cells preliminarily filtered through a column of cotton. Anticerebral sera proved to react strictly specifically with the thymus-dependent population of lymphoid cells; antithymus sera behaved in analogous way both against the intact and against the filtered thymocytes. The action spectrum of the sera against the cells of the lymph nodes and the spleen depended on the methof obtained them. The sera against the intact cells of the lymph nodes reacted chiefly with the T-cells, killing 20% of the cells of bone marrow origin. The sera against the lymph node cells enriched by filtration were strictly specific; in comparison with the sera against crude cells, the sera against filtered splenic cells contained much more antibodies reacting with T-cells; admixture of antibodies against B cells was the same in the sera against crude and against filtered cells. Immunization of rabbits with mouse bone marrow cells permitted to obtain antiserum which gave a strictly specific reaction with B-cells after a corresponding absorption with thymocytes.", "contents": "[Comparative characteristics of the cytotoxic activity of antibrain and different antilymphocyte sera in vitro]. The cytotoxic test was used to assess the specificity of the action of antisera obtained by immunization of rabbits with the tissue of mouse brain, mouse intact lymphoid cells of various origin, and cells preliminarily filtered through a column of cotton. Anticerebral sera proved to react strictly specifically with the thymus-dependent population of lymphoid cells; antithymus sera behaved in analogous way both against the intact and against the filtered thymocytes. The action spectrum of the sera against the cells of the lymph nodes and the spleen depended on the methof obtained them. The sera against the intact cells of the lymph nodes reacted chiefly with the T-cells, killing 20% of the cells of bone marrow origin. The sera against the lymph node cells enriched by filtration were strictly specific; in comparison with the sera against crude cells, the sera against filtered splenic cells contained much more antibodies reacting with T-cells; admixture of antibodies against B cells was the same in the sera against crude and against filtered cells. Immunization of rabbits with mouse bone marrow cells permitted to obtain antiserum which gave a strictly specific reaction with B-cells after a corresponding absorption with thymocytes.", "PMID": 961263}
{"id": "pubmed23n0032_4270", "title": "[Repeated immune response in gnotobiotic rats].", "content": "Repeated immune response to diphtheria toxoid was investigated in monocontaminated rats. It was shown that repeated immunization of these animals led to a sufficiently intensive immune response by the secondary type, but it ended much earlier than in the usual animals (by the 12th day). A supposition is put forward that earlier termination of the secondary response in gnotobionts is caused by the absence in their lymph nodes of an additional differentiation of cell-precursors at the late periods of secondary immune response.", "contents": "[Repeated immune response in gnotobiotic rats]. Repeated immune response to diphtheria toxoid was investigated in monocontaminated rats. It was shown that repeated immunization of these animals led to a sufficiently intensive immune response by the secondary type, but it ended much earlier than in the usual animals (by the 12th day). A supposition is put forward that earlier termination of the secondary response in gnotobionts is caused by the absence in their lymph nodes of an additional differentiation of cell-precursors at the late periods of secondary immune response.", "PMID": 961264}
{"id": "pubmed23n0032_4271", "title": "[Effect of cholerogen on tissue culture cells].", "content": "A study was made of various series of cholerogen on the cultures of human continuous cells of normal (F1, Rh) and tumour nature (HeLa). Cholerogen proved to produce a marked toxic action on the cultures of F1, Rh and HeLa cells causing a reduction of the number of living cells, depression of mitotic activity, a reduction of the intensity of staining on the sum total protein and RNA, a reduction of the activity in the cells of acid phosphatase and succinic dehydrogenase, and also a reduction of production of protein-polysaccharide layer. Different cholerogens produced a different toxic action on the cells of the same type.", "contents": "[Effect of cholerogen on tissue culture cells]. A study was made of various series of cholerogen on the cultures of human continuous cells of normal (F1, Rh) and tumour nature (HeLa). Cholerogen proved to produce a marked toxic action on the cultures of F1, Rh and HeLa cells causing a reduction of the number of living cells, depression of mitotic activity, a reduction of the intensity of staining on the sum total protein and RNA, a reduction of the activity in the cells of acid phosphatase and succinic dehydrogenase, and also a reduction of production of protein-polysaccharide layer. Different cholerogens produced a different toxic action on the cells of the same type.", "PMID": 961265}
{"id": "pubmed23n0032_4272", "title": "[Etiologic role of klebsiella in children with acute intestinal diseases].", "content": "A study was made of possible etiological association between klebsiellae and acute enteric diseases in children of creches age. The mean frequency of isolation of klebsiellae from these children constituted 22.3%, and from somatic patients - 38.5%. Both in the patients with enteric diseases and in somatic patients with intestinal disturbances the appearance of klebsiellae was preceded by oher diseases or by prolonged treatment with antibiotics leading to reduction of general resistance of the organism. Clinical manifestations were unitypical in the patients of both categories. In healthy children the incidence of klebsiellae was less (4.7%) and without any clinical manifestations. I is suggested that there is an etiological association between klebsiellae and acute enteric diseases under definite conditons.", "contents": "[Etiologic role of klebsiella in children with acute intestinal diseases]. A study was made of possible etiological association between klebsiellae and acute enteric diseases in children of creches age. The mean frequency of isolation of klebsiellae from these children constituted 22.3%, and from somatic patients - 38.5%. Both in the patients with enteric diseases and in somatic patients with intestinal disturbances the appearance of klebsiellae was preceded by oher diseases or by prolonged treatment with antibiotics leading to reduction of general resistance of the organism. Clinical manifestations were unitypical in the patients of both categories. In healthy children the incidence of klebsiellae was less (4.7%) and without any clinical manifestations. I is suggested that there is an etiological association between klebsiellae and acute enteric diseases under definite conditons.", "PMID": 961268}
{"id": "pubmed23n0032_4273", "title": "[Characteristics of strains of Citrobacter 037 (5396/38) isolated from guinea pigs in a gnotobiologic experiment].", "content": "No changes in the principal biochemical properties characteristic of the given genus occurred in a gnotobiological experiment of passing Citrobacter bacteria through microbe free and exmicrobe-free animals. Of 28 strains of this genus isolated in the course of investigation two strains lost their arginine-decarboxylase activity. In the cultures studied the quantitative content of the Vi-antigen had a tendency to reduction in comparison with the initial culture used for the association. some of the strains studied had one more common antigen with typhoid bacilli (strain Ty2-4446) along with theknown cross by the Vi-antigen. Bacteria of the Enterobacter and Excherichia genus isolated after the conventionalization had common antigens with typhoid bacilli (Ty2-4446).", "contents": "[Characteristics of strains of Citrobacter 037 (5396/38) isolated from guinea pigs in a gnotobiologic experiment]. No changes in the principal biochemical properties characteristic of the given genus occurred in a gnotobiological experiment of passing Citrobacter bacteria through microbe free and exmicrobe-free animals. Of 28 strains of this genus isolated in the course of investigation two strains lost their arginine-decarboxylase activity. In the cultures studied the quantitative content of the Vi-antigen had a tendency to reduction in comparison with the initial culture used for the association. some of the strains studied had one more common antigen with typhoid bacilli (strain Ty2-4446) along with theknown cross by the Vi-antigen. Bacteria of the Enterobacter and Excherichia genus isolated after the conventionalization had common antigens with typhoid bacilli (Ty2-4446).", "PMID": 961269}
{"id": "pubmed23n0032_4274", "title": "[Immunologic reactions in man following oral immunization].", "content": "Prolonged clinico-immunological investigation was carried out on a volunteer; it was shown that a single oral vaccination with living B. citrobacter culture produced a marked immunological reaction in the form of increase of the typhoid Vi- and O-coproantibodies revealed in the fecal filtrate. The greatest rise of the preventive activity of the blood serum was noted after a 4-fold vaccination, whereas the maximum antibody formation--after a five-fold immunization in doses tested in the experiment. There was revealed no correlation (in time or intensity) between the three tests with the aid of which was the oral vaccination assessed.", "contents": "[Immunologic reactions in man following oral immunization]. Prolonged clinico-immunological investigation was carried out on a volunteer; it was shown that a single oral vaccination with living B. citrobacter culture produced a marked immunological reaction in the form of increase of the typhoid Vi- and O-coproantibodies revealed in the fecal filtrate. The greatest rise of the preventive activity of the blood serum was noted after a 4-fold vaccination, whereas the maximum antibody formation--after a five-fold immunization in doses tested in the experiment. There was revealed no correlation (in time or intensity) between the three tests with the aid of which was the oral vaccination assessed.", "PMID": 961270}
{"id": "pubmed23n0032_4275", "title": "[Antigenic structure of non-agglutinating vibrios and bacteria of the genus Aeromonas].", "content": "The authors carried out a comparative study of the antigenic structure of nonagglutinating vibrios and the respresentatives of Aeromonas genus. A number of common antigens (some of which were of protein origin) were revealed in the so-called enterotoxins of nonaggultinating vibrios and aeromonas and also in the cytoplasm and ribosomes of these strains. \"Enterotoxin\" of Aeromona s strain contained the factor of vascular permeability. The serum to \"enterotoxin\" of the Aeromonas strain neutralized the enterotoxin of nonagglutinating vibrio.", "contents": "[Antigenic structure of non-agglutinating vibrios and bacteria of the genus Aeromonas]. The authors carried out a comparative study of the antigenic structure of nonagglutinating vibrios and the respresentatives of Aeromonas genus. A number of common antigens (some of which were of protein origin) were revealed in the so-called enterotoxins of nonaggultinating vibrios and aeromonas and also in the cytoplasm and ribosomes of these strains. \"Enterotoxin\" of Aeromona s strain contained the factor of vascular permeability. The serum to \"enterotoxin\" of the Aeromonas strain neutralized the enterotoxin of nonagglutinating vibrio.", "PMID": 961271}
{"id": "pubmed23n0032_4276", "title": "[Indices of phagocytosis of staphylococci with different biological activity].", "content": "An index of completion of phagocytosis was studied for staphylococcus cultures of various biological activity in experiments with surviving polymorphonuclear leukocytes. Among the coagulase-forming staphylococci virulent strains proved to be nonviable in a lesser percentage of cases than the avirulent ones. Coagulase- and mannite-negative cultures proved to be most unstable. Staphylococcus toxin depressed the migration of polymorphonuclear leukocytes to the focus of irritation, inhibited ingestion of the virulent, and, to a lesser degree, of avirulent coagulase-forming staphylococci, but failed to influence the ingestion of coagulase-negative epidermal staphylococci. The influence of staphylotoxin on the phagocytosis completion index was either the minimal or absent entirely.", "contents": "[Indices of phagocytosis of staphylococci with different biological activity]. An index of completion of phagocytosis was studied for staphylococcus cultures of various biological activity in experiments with surviving polymorphonuclear leukocytes. Among the coagulase-forming staphylococci virulent strains proved to be nonviable in a lesser percentage of cases than the avirulent ones. Coagulase- and mannite-negative cultures proved to be most unstable. Staphylococcus toxin depressed the migration of polymorphonuclear leukocytes to the focus of irritation, inhibited ingestion of the virulent, and, to a lesser degree, of avirulent coagulase-forming staphylococci, but failed to influence the ingestion of coagulase-negative epidermal staphylococci. The influence of staphylotoxin on the phagocytosis completion index was either the minimal or absent entirely.", "PMID": 961272}
{"id": "pubmed23n0032_4277", "title": "[Experimental latent typhus].", "content": "Asymptomatic typhus infection was regularly modelled in guinea pigs with increased resistance to Rickettsia prowazeki; this infection was characterized by the presence of immunological shifts in the blood and immunity to reinfection, persistenc of rickettsia in the organs of animals in the absence of pyretic reaction and periorchites. Modelling in guinea pigs of asymptomatic infection is also possible against the background of passive immunity created by specific immune serum obtained during convalescence. Investigations demonstrated that rickettsiocidal activity (in experiments on chick embryos) and antiinfectious activity (in experiments on guinea pigs) of the sera was caused by 7S-antibodies.", "contents": "[Experimental latent typhus]. Asymptomatic typhus infection was regularly modelled in guinea pigs with increased resistance to Rickettsia prowazeki; this infection was characterized by the presence of immunological shifts in the blood and immunity to reinfection, persistenc of rickettsia in the organs of animals in the absence of pyretic reaction and periorchites. Modelling in guinea pigs of asymptomatic infection is also possible against the background of passive immunity created by specific immune serum obtained during convalescence. Investigations demonstrated that rickettsiocidal activity (in experiments on chick embryos) and antiinfectious activity (in experiments on guinea pigs) of the sera was caused by 7S-antibodies.", "PMID": 961273}
{"id": "pubmed23n0032_4278", "title": "[Use of Soviet semipermeable acetate-cellulose membranes for concentration and purification of typhoid antigens].", "content": "A study was made of a possibility of using Soviet made membranes \"Vladipor\" with a pore diameter of 16--17 nm for the purpose of concentration and purification of antigenic extracts from stab cultures of S. typhi obtained by the action of typsin, hydrogen peroxide and detergents. Preparations, complete by antigenic structure, were obtained by ultrafiltration, with a good yield. The maximal rate of ultrafiltration was noted in case of \"peroxide\" antigenic preparations. The use of diafiltration provided a significant reduction of the ash content in the end products.", "contents": "[Use of Soviet semipermeable acetate-cellulose membranes for concentration and purification of typhoid antigens]. A study was made of a possibility of using Soviet made membranes \"Vladipor\" with a pore diameter of 16--17 nm for the purpose of concentration and purification of antigenic extracts from stab cultures of S. typhi obtained by the action of typsin, hydrogen peroxide and detergents. Preparations, complete by antigenic structure, were obtained by ultrafiltration, with a good yield. The maximal rate of ultrafiltration was noted in case of \"peroxide\" antigenic preparations. The use of diafiltration provided a significant reduction of the ash content in the end products.", "PMID": 961274}
{"id": "pubmed23n0032_4279", "title": "[Change in the non-specific resistance of the body to influenza and acute respiratory diseases following immunization diphtheria-tetanus vaccine].", "content": "A single vaccination of animals with diphtheria-tetanus vaccine caused changes in non-specific resistance of the organism to influenza of phasic character: a reduction of resistance to influenza was observed at the early periods after the vaccination, with its subsequent restoration to the initial level and increase at the remote periods after the immunization. Immunization of children with diphtheria-tetanus vaccine also led to a short-term reduction non-specific resistance to influenza and acute respiratory diseases during the second decade after the vaccination, and its subsequent increase at periods from the 40th to the 60th day.", "contents": "[Change in the non-specific resistance of the body to influenza and acute respiratory diseases following immunization diphtheria-tetanus vaccine]. A single vaccination of animals with diphtheria-tetanus vaccine caused changes in non-specific resistance of the organism to influenza of phasic character: a reduction of resistance to influenza was observed at the early periods after the vaccination, with its subsequent restoration to the initial level and increase at the remote periods after the immunization. Immunization of children with diphtheria-tetanus vaccine also led to a short-term reduction non-specific resistance to influenza and acute respiratory diseases during the second decade after the vaccination, and its subsequent increase at periods from the 40th to the 60th day.", "PMID": 961275}
{"id": "pubmed23n0032_4280", "title": "[Methods of detecting the agent of melioidosis in objects of the external environment and other material].", "content": "On the basis of literature data and personal investigations the authors elaborated a scheme of detection of the causative agent of melioidosis in the objects of external environment, including fluorescent microscopy of crude material, seeding on meat-peptone agar with crystal violet, seeding material treated with penicillin on fluid medium with subsequent transfer on solid Levin medium and infection of animals, seeding on a medium with beta-alanine and neonmycin and infection of sensitive animals with crude material.", "contents": "[Methods of detecting the agent of melioidosis in objects of the external environment and other material]. On the basis of literature data and personal investigations the authors elaborated a scheme of detection of the causative agent of melioidosis in the objects of external environment, including fluorescent microscopy of crude material, seeding on meat-peptone agar with crystal violet, seeding material treated with penicillin on fluid medium with subsequent transfer on solid Levin medium and infection of animals, seeding on a medium with beta-alanine and neonmycin and infection of sensitive animals with crude material.", "PMID": 961276}
{"id": "pubmed23n0032_4281", "title": "[Palatine tonsils and immunity. VIII. Soluble factors produced by lymphocytes of the palatine tonsils and other lymphoid formations in rabbits].", "content": "A comparison was made of the activity of solbule factors produced by thelymphocytes of the palatine tonsils, the appendix, the spleen and thelymph nodes of rabbits in stimulation with a specific antigen (complete antigen of streptococcus, group A) and PHA. The blastogenic factor produced  by the lymphoid cells of the spleen and the lymph nodes was highly active. Lymphocytes of the palatine tonsils and of the appendix produced a factor withlow activity after PHA stimulation. A factor intensifying the transformation in the presence of a complete streptococcus antigen was produced by lymphocytes of the spleen and the lymph nodes of the immunized animals. The lymphocytes of the palatine tonsils and the appendix failed to produce this factor.", "contents": "[Palatine tonsils and immunity. VIII. Soluble factors produced by lymphocytes of the palatine tonsils and other lymphoid formations in rabbits]. A comparison was made of the activity of solbule factors produced by thelymphocytes of the palatine tonsils, the appendix, the spleen and thelymph nodes of rabbits in stimulation with a specific antigen (complete antigen of streptococcus, group A) and PHA. The blastogenic factor produced  by the lymphoid cells of the spleen and the lymph nodes was highly active. Lymphocytes of the palatine tonsils and of the appendix produced a factor withlow activity after PHA stimulation. A factor intensifying the transformation in the presence of a complete streptococcus antigen was produced by lymphocytes of the spleen and the lymph nodes of the immunized animals. The lymphocytes of the palatine tonsils and the appendix failed to produce this factor.", "PMID": 961277}
{"id": "pubmed23n0032_4282", "title": "[Use of the passive hemagglutination test for diagnosis of meningococcal infection].", "content": "A considerable percentage of patients with meningococcus infection displayed an increase in the actibody titres to meningococcus, group A, detectable in the passive hemagglutination test (PHAT). An increase in the antibody titre was mose frequent in the patients with clinical manifestations of meningococciemia and meningitis. The PHAT can be used as an auxiliary method of diagnosis permitting to establish the meningococcus etiology of the disease in a number of cases.", "contents": "[Use of the passive hemagglutination test for diagnosis of meningococcal infection]. A considerable percentage of patients with meningococcus infection displayed an increase in the actibody titres to meningococcus, group A, detectable in the passive hemagglutination test (PHAT). An increase in the antibody titre was mose frequent in the patients with clinical manifestations of meningococciemia and meningitis. The PHAT can be used as an auxiliary method of diagnosis permitting to establish the meningococcus etiology of the disease in a number of cases.", "PMID": 961278}
{"id": "pubmed23n0032_4283", "title": "[Experimental model of transient cerebral circulatory disorders by way of embolization of cerebral arteries with fresh blood clots].", "content": "A total of 85 experimental animals received through the blood flux of the carotid arteries clots of autogenic blood of 1 hour age. A histomorphological study after 40 minutes following an embolism there were revealed clots in the cerebral arteries in 13 out of 14 animals. After 1.5 hours in 1 of 17, and after 24 hours in none of the 10 animals. Directly following the administration of clots in all the 20 animals of this series, there were symptoms of focal brain lesions: hemipareses, ptosis and myosis, a \"trot around circles\", etc. In all 20 animals there was a complete regress of the symptom at different times (from 10 min to 18 hours). In all cases there was an increased amount of lactic acid in the cerebral blood outflow while subsequently was reduced, correlating with the time of clot lysis. The morphological control did not depict brain infarctions. It is assumed that the reason of brain dysfunctions is an obturation by blood clots of cerebral arteries, while their normalization is due to a spontaneous lysis of the embols which obturated the cerebral arteries.", "contents": "[Experimental model of transient cerebral circulatory disorders by way of embolization of cerebral arteries with fresh blood clots]. A total of 85 experimental animals received through the blood flux of the carotid arteries clots of autogenic blood of 1 hour age. A histomorphological study after 40 minutes following an embolism there were revealed clots in the cerebral arteries in 13 out of 14 animals. After 1.5 hours in 1 of 17, and after 24 hours in none of the 10 animals. Directly following the administration of clots in all the 20 animals of this series, there were symptoms of focal brain lesions: hemipareses, ptosis and myosis, a \"trot around circles\", etc. In all 20 animals there was a complete regress of the symptom at different times (from 10 min to 18 hours). In all cases there was an increased amount of lactic acid in the cerebral blood outflow while subsequently was reduced, correlating with the time of clot lysis. The morphological control did not depict brain infarctions. It is assumed that the reason of brain dysfunctions is an obturation by blood clots of cerebral arteries, while their normalization is due to a spontaneous lysis of the embols which obturated the cerebral arteries.", "PMID": 961280}
{"id": "pubmed23n0032_4284", "title": "[Thrombocyte lactate dehydrogenase isoenzymes in patients with ischemic stroke].", "content": "It was established that in the acute period of the ischemic stroke in the maiority of cases a change of isoenzyme structure of LDG thrombocytes was due to a decrease of LDG3 activity. In a number of patients the dominant change in the spectrum was determined by LDG and LDG4 fractions. The changes were of a stable character up to 10 weeks. LDG4 increase was characteristic of the isoenzymes spectrum in the patients with a severe development of the disease and in the patients in the remote period of the stroke. The obtained data can serve as a criterion for evaluating the adaptive changes in acute brain ischemia.", "contents": "[Thrombocyte lactate dehydrogenase isoenzymes in patients with ischemic stroke]. It was established that in the acute period of the ischemic stroke in the maiority of cases a change of isoenzyme structure of LDG thrombocytes was due to a decrease of LDG3 activity. In a number of patients the dominant change in the spectrum was determined by LDG and LDG4 fractions. The changes were of a stable character up to 10 weeks. LDG4 increase was characteristic of the isoenzymes spectrum in the patients with a severe development of the disease and in the patients in the remote period of the stroke. The obtained data can serve as a criterion for evaluating the adaptive changes in acute brain ischemia.", "PMID": 961281}
{"id": "pubmed23n0032_4285", "title": "[Comparative concentration of serum free amino acids in patients with incipient and manifest organic neuropsychiatric disorders of vascular origin].", "content": "The authors conducted a study of the quantitative content of amino acids in the blood serum of patients with initial and expressed organic neuro-psychic disorders of a vascular genesis and in a group of normals of the same age. In patients with initial organic syndromes the content of cystin-cystein and treonine in the blood serum increased. In patients with expressed organic syndromes the content of cystin-cystein increased, while the content of arginine acid and leicyn-isoleicyn decreased as compared to the control group of elderly people. A comparative study of the two groups of patients with organic psychopathology of a vascular origin demonstrated that in vascular dementia there are more severe changes in the content of amino acids in the blood serum.", "contents": "[Comparative concentration of serum free amino acids in patients with incipient and manifest organic neuropsychiatric disorders of vascular origin]. The authors conducted a study of the quantitative content of amino acids in the blood serum of patients with initial and expressed organic neuro-psychic disorders of a vascular genesis and in a group of normals of the same age. In patients with initial organic syndromes the content of cystin-cystein and treonine in the blood serum increased. In patients with expressed organic syndromes the content of cystin-cystein increased, while the content of arginine acid and leicyn-isoleicyn decreased as compared to the control group of elderly people. A comparative study of the two groups of patients with organic psychopathology of a vascular origin demonstrated that in vascular dementia there are more severe changes in the content of amino acids in the blood serum.", "PMID": 961282}
{"id": "pubmed23n0032_4286", "title": "[Combined quantitative analysis of electro- and rheoencephalograms at different ages and features of their individual indices with use of vasoactive drugs].", "content": "The polygraphic studies with the calculation of the complex of the EEG and REG quantitative parameters which were conducted in 20 individuals of young age and 20 adult individuals, displayed that there was an increase of the vascular tone and a decrease of the brain pulse blood-content which were observed not rarely with the EEG irritation at the same time in the mature age. Papaverine and euphilline action revealed dependence on the initial tone of the brain vessels and expressiveness of the general hemodynamic shifts.", "contents": "[Combined quantitative analysis of electro- and rheoencephalograms at different ages and features of their individual indices with use of vasoactive drugs]. The polygraphic studies with the calculation of the complex of the EEG and REG quantitative parameters which were conducted in 20 individuals of young age and 20 adult individuals, displayed that there was an increase of the vascular tone and a decrease of the brain pulse blood-content which were observed not rarely with the EEG irritation at the same time in the mature age. Papaverine and euphilline action revealed dependence on the initial tone of the brain vessels and expressiveness of the general hemodynamic shifts.", "PMID": 961283}
{"id": "pubmed23n0032_4287", "title": "[Role of interruption of the circle of Willis in the genesis of hemorrhagic stroke].", "content": "The author describes the anatomical findings among the variants of brain arteries in 360 deceased where 160 of them had a hemorrhagic or ischemic stroke, while 200 had no brain diseases. Those cases where strokes were revealed the author found a separation in the posterior part of the circle of Willis--between the carotid and vertebro-basillar systems. This could happen due to an aplasia of one or both posterior connective arteries, or due to the absence of a connecting vessel between an atypically diverging posterior brain artery (posterior trifurcation) and the basillar artery. It is assumed that this circumstance has a definite significance in the pathogenesis of hemorrhages.", "contents": "[Role of interruption of the circle of Willis in the genesis of hemorrhagic stroke]. The author describes the anatomical findings among the variants of brain arteries in 360 deceased where 160 of them had a hemorrhagic or ischemic stroke, while 200 had no brain diseases. Those cases where strokes were revealed the author found a separation in the posterior part of the circle of Willis--between the carotid and vertebro-basillar systems. This could happen due to an aplasia of one or both posterior connective arteries, or due to the absence of a connecting vessel between an atypically diverging posterior brain artery (posterior trifurcation) and the basillar artery. It is assumed that this circumstance has a definite significance in the pathogenesis of hemorrhages.", "PMID": 961284}
{"id": "pubmed23n0032_4288", "title": "[Role of craniocerebral trauma in the development of Alzheimer's disease].", "content": "The report deals with a clinico-anatomical description of 3 cases of Alzheimer's disease, developing after a brain trauma. The evolution of the disease justifies the question concerning the role of traumas in the pathogenesis. The atrophical process, lying at the basis of the disease is considered in the aspect of the aging of the colloid brain system under the influence of a mechanical action of a trauma.", "contents": "[Role of craniocerebral trauma in the development of Alzheimer's disease]. The report deals with a clinico-anatomical description of 3 cases of Alzheimer's disease, developing after a brain trauma. The evolution of the disease justifies the question concerning the role of traumas in the pathogenesis. The atrophical process, lying at the basis of the disease is considered in the aspect of the aging of the colloid brain system under the influence of a mechanical action of a trauma.", "PMID": 961285}
{"id": "pubmed23n0032_4289", "title": "[Hippocampal pathology as one of the possible factors in the pathogenesis of several dementias of old age].", "content": "In the light of comtemporary concepts concerning the functional significance of the limbic system, the author reports of some results in the subiculum in young and old individuals (39 cases) with different forms of dementia of old age (senile and vascular dementia, Pick's and Alzheimer's disease). The authors assessed the thickness of the subicular cortical stratum, the relative and absolute amount of nervous and glial cells and capillaries. In old age the thickness of the subicular cortical stratum is decreased by 28%, while the absolute amount of neurons by 29%. In old age dementia the thickness of the subicular cortical stratum decreased by 47--71% and the absolute amount of neurons becomes 2--3 times less. In old age in general and in dementia of old age in particular there was no \"substitutional\" gliosis because the absolute amount of glial cells in the cortical stratum is reduced in this group of individuals by 20--50%.", "contents": "[Hippocampal pathology as one of the possible factors in the pathogenesis of several dementias of old age]. In the light of comtemporary concepts concerning the functional significance of the limbic system, the author reports of some results in the subiculum in young and old individuals (39 cases) with different forms of dementia of old age (senile and vascular dementia, Pick's and Alzheimer's disease). The authors assessed the thickness of the subicular cortical stratum, the relative and absolute amount of nervous and glial cells and capillaries. In old age the thickness of the subicular cortical stratum is decreased by 28%, while the absolute amount of neurons by 29%. In old age dementia the thickness of the subicular cortical stratum decreased by 47--71% and the absolute amount of neurons becomes 2--3 times less. In old age in general and in dementia of old age in particular there was no \"substitutional\" gliosis because the absolute amount of glial cells in the cortical stratum is reduced in this group of individuals by 20--50%.", "PMID": 961286}
{"id": "pubmed23n0032_4290", "title": "[Features of episodes of mental disorders in chronic renal insufficiency (clinico-morphologic study)].", "content": "The authors studied the traits of psychotic attacks in 40 patients with terminal stages of chronic renal insufficiency. They were expressed in atypical delirious, delirio-amentive and amentive conditions. 8 cases were studied morphologically. The pathological process in the brain was characterized as toxico-dyshomeostatic encephalopathy. The pathogenesis of such disorders is discussed.", "contents": "[Features of episodes of mental disorders in chronic renal insufficiency (clinico-morphologic study)]. The authors studied the traits of psychotic attacks in 40 patients with terminal stages of chronic renal insufficiency. They were expressed in atypical delirious, delirio-amentive and amentive conditions. 8 cases were studied morphologically. The pathological process in the brain was characterized as toxico-dyshomeostatic encephalopathy. The pathogenesis of such disorders is discussed.", "PMID": 961287}
{"id": "pubmed23n0032_4291", "title": "[Changes in the ultra-fine structure of capillaries of the embryonic brain in the presence of schizophrenia in the mother].", "content": "The report is concerned with a study of fine structures of brain capillaries in 9 embryos received during medical abortions from schizophrenic mothers. The authors established some traits in the ultrastructure of endothelial cells in most of the studied cases--an increase of the surface of endothelial cells at the expense of a tortuosity of their plasmatic membranes and a formation of growths, vacuolization of the cytoplasma. There were also some changes in the structure of basal membranes. In 2 cases there were also peculiar pathological changes in the vascular endothelium: granules of an unknown nature in the endothelial cells and a focal lysis of the plasmatic membranes of endothelial cells.", "contents": "[Changes in the ultra-fine structure of capillaries of the embryonic brain in the presence of schizophrenia in the mother]. The report is concerned with a study of fine structures of brain capillaries in 9 embryos received during medical abortions from schizophrenic mothers. The authors established some traits in the ultrastructure of endothelial cells in most of the studied cases--an increase of the surface of endothelial cells at the expense of a tortuosity of their plasmatic membranes and a formation of growths, vacuolization of the cytoplasma. There were also some changes in the structure of basal membranes. In 2 cases there were also peculiar pathological changes in the vascular endothelium: granules of an unknown nature in the endothelial cells and a focal lysis of the plasmatic membranes of endothelial cells.", "PMID": 961288}
{"id": "pubmed23n0032_4292", "title": "[Morphologic characteristics of the pituitary-adrenal system in schizophrenia (according to early autopsy findings)].", "content": "The presented data are concerned with the morphology of the hypophysis (neurohypophysis, intermittent lobe and adenohypophysis) and cortical layer of the adrenals in 30 schizophrenic patients with different forms of the disease, duration and causes of lethal outcomes. The studied material was taken during the first 6 hours following death. The author gives an assessment of the functions in separate glands (different variants in the morphological picture) and the hypophysis adrenal system in general. In 60% of the cases there was a sufficient activity of the studied glands (including cases where the morphology of the glands was close to normal conditions, increased activity and exhaustion after an increased functional tension).", "contents": "[Morphologic characteristics of the pituitary-adrenal system in schizophrenia (according to early autopsy findings)]. The presented data are concerned with the morphology of the hypophysis (neurohypophysis, intermittent lobe and adenohypophysis) and cortical layer of the adrenals in 30 schizophrenic patients with different forms of the disease, duration and causes of lethal outcomes. The studied material was taken during the first 6 hours following death. The author gives an assessment of the functions in separate glands (different variants in the morphological picture) and the hypophysis adrenal system in general. In 60% of the cases there was a sufficient activity of the studied glands (including cases where the morphology of the glands was close to normal conditions, increased activity and exhaustion after an increased functional tension).", "PMID": 961289}
{"id": "pubmed23n0032_4293", "title": "[Ultrastructure of schizophrenic patients' lymph nodes].", "content": "By means of the electron microscopic method it became possible to study the structure of the lymph groin node (bioptic material) in 4 cases of schizophrenia and in 2 normals. All the schizophrenic patients had continuous forms (nuclear) of the disease, while their mental state during the period of examination was characterized as terminal. An analysis of the cell structure in the lymph node demonstrated that in both groups the functional activity of the lymphoid tissue was sufficiently high and in relation to some indices this activity was even higher in the group of schizophrenics (a more expressed pynocytose activity of the reticular cells and macrophages, a higher amount of blast forms of the lymphocytes, plasmatic cells and activated lymphocytes). The authors stress that these data are purely preliminary.", "contents": "[Ultrastructure of schizophrenic patients' lymph nodes]. By means of the electron microscopic method it became possible to study the structure of the lymph groin node (bioptic material) in 4 cases of schizophrenia and in 2 normals. All the schizophrenic patients had continuous forms (nuclear) of the disease, while their mental state during the period of examination was characterized as terminal. An analysis of the cell structure in the lymph node demonstrated that in both groups the functional activity of the lymphoid tissue was sufficiently high and in relation to some indices this activity was even higher in the group of schizophrenics (a more expressed pynocytose activity of the reticular cells and macrophages, a higher amount of blast forms of the lymphocytes, plasmatic cells and activated lymphocytes). The authors stress that these data are purely preliminary.", "PMID": 961290}
{"id": "pubmed23n0032_4294", "title": "[Quantitative electron-microscopic analysis of the lymphocyte population in the peripheral blood of schizophrenic patients].", "content": "The author conducted a quantitative submicroscopic analysis of a population of recently isolated lymphocytes in the peripheral blood of schizophrenic patients. At the basis of the analysis there lies a subdivision of lymphocytes into 3 types according to their ultrastructure: narrow-plasmatic (I), widerplasmatic (II) and activated (III) cells. In the blood of schizophrenic patients the correlations of these cells were 54%, 24% and 22%, while the same figures for normals were 63%, 24% and 13%. These data may indicate that in schizophrenia the peripheral blood contains 1.7 times more activated lymphocytes than in normals. The activated lymphocytes possess an increased adhesiveness. They probably are atypical lymphocytes in the peripheral blood of schizophrenic patients which can be seen through an optical microscope.", "contents": "[Quantitative electron-microscopic analysis of the lymphocyte population in the peripheral blood of schizophrenic patients]. The author conducted a quantitative submicroscopic analysis of a population of recently isolated lymphocytes in the peripheral blood of schizophrenic patients. At the basis of the analysis there lies a subdivision of lymphocytes into 3 types according to their ultrastructure: narrow-plasmatic (I), widerplasmatic (II) and activated (III) cells. In the blood of schizophrenic patients the correlations of these cells were 54%, 24% and 22%, while the same figures for normals were 63%, 24% and 13%. These data may indicate that in schizophrenia the peripheral blood contains 1.7 times more activated lymphocytes than in normals. The activated lymphocytes possess an increased adhesiveness. They probably are atypical lymphocytes in the peripheral blood of schizophrenic patients which can be seen through an optical microscope.", "PMID": 961291}
{"id": "pubmed23n0032_4295", "title": "[Clinico-morphologic characteristics of cases of alcoholic delirium with lethal outcome].", "content": "The authors conducted a clinico-morphogicaly analysis of 12 cases (10 males and 2 females) of alcoholic delirium with a lethal outcome. All 12 patients were alcohol abusers (from 5--22 years). Pathoanatomical changes of the brain and internal organs were characterized by traits of chronic alcoholic encephalopathy accompanied by elements of toxic damage of the nervous tissue. The main factor in the genesis of this severe disorder is a diffuse acute toxic damage of the minor vessels of the cortex and white brain matter (more expressed in the frontal areas of the hemispheres). The authors discuss the pathogenetical role of hepatic lesions in alcoholic delirium.", "contents": "[Clinico-morphologic characteristics of cases of alcoholic delirium with lethal outcome]. The authors conducted a clinico-morphogicaly analysis of 12 cases (10 males and 2 females) of alcoholic delirium with a lethal outcome. All 12 patients were alcohol abusers (from 5--22 years). Pathoanatomical changes of the brain and internal organs were characterized by traits of chronic alcoholic encephalopathy accompanied by elements of toxic damage of the nervous tissue. The main factor in the genesis of this severe disorder is a diffuse acute toxic damage of the minor vessels of the cortex and white brain matter (more expressed in the frontal areas of the hemispheres). The authors discuss the pathogenetical role of hepatic lesions in alcoholic delirium.", "PMID": 961292}
{"id": "pubmed23n0032_4296", "title": "[Thiamine deficit in acute alcoholic psychoses].", "content": "In 35 females and 22 males with acute alcoholic disorders and in 44 individuals of a control group the author studied the indices of thiamine balance--the content of thiamine and pyruvic acid in the blood and urine, concentration of citric acid in the blood, activity of transketolase of erythrocytes and TDP-effect. The results demonstrated that a thiamide deficiency in excretion and insufficiency in coenzyme functions of oxidative decarboxylation of pyruvic acid and in the pentose cycle is seen not only in alcoholic psychoses, but in the withdrawal syndrome. Sex differences of these indices and their development were not noted. It is assumed that the clinical traits of alcoholic disorders in females are not related to changes in the thiamine metabolism.", "contents": "[Thiamine deficit in acute alcoholic psychoses]. In 35 females and 22 males with acute alcoholic disorders and in 44 individuals of a control group the author studied the indices of thiamine balance--the content of thiamine and pyruvic acid in the blood and urine, concentration of citric acid in the blood, activity of transketolase of erythrocytes and TDP-effect. The results demonstrated that a thiamide deficiency in excretion and insufficiency in coenzyme functions of oxidative decarboxylation of pyruvic acid and in the pentose cycle is seen not only in alcoholic psychoses, but in the withdrawal syndrome. Sex differences of these indices and their development were not noted. It is assumed that the clinical traits of alcoholic disorders in females are not related to changes in the thiamine metabolism.", "PMID": 961293}
{"id": "pubmed23n0032_4297", "title": "[Clinico-biochemical study of epileptic children and adolescents and their relatives].", "content": "The observations were convened in the framework of clinico-genetical study of epilepsy. It was established that in the blood serum of epileptic children and adolescents and their relatives there is an increase of general activity of LDH. With the aid of electrophoresis in agar and polyacrylamid gel it was possible to show an increase in the content of LDH-1 and a drop in LDH-3. These shifts are more expressed in the blood serum of patients and sibs, rather than in parents. It is assumed that an increase of general LDH and LDH-1 activity are conditioned by an increased membrane permeability of brain cells. There were no differences in the degree of these indices depending upon the genesis, sex and frequency of paroxysms.", "contents": "[Clinico-biochemical study of epileptic children and adolescents and their relatives]. The observations were convened in the framework of clinico-genetical study of epilepsy. It was established that in the blood serum of epileptic children and adolescents and their relatives there is an increase of general activity of LDH. With the aid of electrophoresis in agar and polyacrylamid gel it was possible to show an increase in the content of LDH-1 and a drop in LDH-3. These shifts are more expressed in the blood serum of patients and sibs, rather than in parents. It is assumed that an increase of general LDH and LDH-1 activity are conditioned by an increased membrane permeability of brain cells. There were no differences in the degree of these indices depending upon the genesis, sex and frequency of paroxysms.", "PMID": 961294}
{"id": "pubmed23n0032_4298", "title": "[Use of the Soviet preparation methindione for injections in convulsive conditions].", "content": "The author studied the action of a Soviet preparation Metindion in 22 patients with serial epileptic seizures and in 16 patients with an epileptic status. The studies demonstrated a high anticonvulsive activity in serial fits. In epileptical states the action of metindion was less expressed. Its combination with diazepam preparations and barbiturates appeared to have a more expressed and prolonged anticonvulsive action. Metindion is most effective when used at initial stages of a convulsive condition.", "contents": "[Use of the Soviet preparation methindione for injections in convulsive conditions]. The author studied the action of a Soviet preparation Metindion in 22 patients with serial epileptic seizures and in 16 patients with an epileptic status. The studies demonstrated a high anticonvulsive activity in serial fits. In epileptical states the action of metindion was less expressed. Its combination with diazepam preparations and barbiturates appeared to have a more expressed and prolonged anticonvulsive action. Metindion is most effective when used at initial stages of a convulsive condition.", "PMID": 961295}
{"id": "pubmed23n0032_4299", "title": "[Value of pendulum-like oculonyorhythms and myorhythms of other muscles for prognosis of restoration of cerebral cortical function following hypoxia].", "content": "In clinical and EEG studies of 92 patients where the preterminal and terminal states were arrested it was possible to demonstrate that the phenomenon of a spontaneous discharge of pendulum-like oculomyorhythms and myorhythms of the orbital muscles is a significantly negative sign pointing to irreversible cortical lesion. Conditioned by oculomyorhythm the artefacts seen in the EEG of comatose patients simulate an intactness of the brain electroactivity and are a source of an erroneous evaluation of the EEG.", "contents": "[Value of pendulum-like oculonyorhythms and myorhythms of other muscles for prognosis of restoration of cerebral cortical function following hypoxia]. In clinical and EEG studies of 92 patients where the preterminal and terminal states were arrested it was possible to demonstrate that the phenomenon of a spontaneous discharge of pendulum-like oculomyorhythms and myorhythms of the orbital muscles is a significantly negative sign pointing to irreversible cortical lesion. Conditioned by oculomyorhythm the artefacts seen in the EEG of comatose patients simulate an intactness of the brain electroactivity and are a source of an erroneous evaluation of the EEG.", "PMID": 961296}
{"id": "pubmed23n0032_4300", "title": "[Quantitative assessment, sex traits and types of chronograms of the Achilles reflex].", "content": "The time of Achille's reflex was measured with the aid of an independent electronic device tirocron in 104 normal natives of the Zambian Republic. In males the time of the reflex was significantly shorter (299.8+/-4.6 msec for the right and 303.4+/-4.9 msec for the left foot) than in mussles (326.1+/-5.6 and 329.9+/-5.9 respectively). Difference in the time of the reflex on the right and left foot in males and females was not noted. The \"warmup phenomenon\" was more frequently seen in females with the same frequency on the right and left feet. Three types of chronograms of Achille's reflex were depicted: stable, relatively stable and disseminated. The stable type prevaled on the right foot as compared to the left one and in males as compared to females. The disseminated type was more frequently seen in females on the left side. In determing the time of Achille's reflex it is necessary to use the arithmetical mean not less than 15--20 measurements.", "contents": "[Quantitative assessment, sex traits and types of chronograms of the Achilles reflex]. The time of Achille's reflex was measured with the aid of an independent electronic device tirocron in 104 normal natives of the Zambian Republic. In males the time of the reflex was significantly shorter (299.8+/-4.6 msec for the right and 303.4+/-4.9 msec for the left foot) than in mussles (326.1+/-5.6 and 329.9+/-5.9 respectively). Difference in the time of the reflex on the right and left foot in males and females was not noted. The \"warmup phenomenon\" was more frequently seen in females with the same frequency on the right and left feet. Three types of chronograms of Achille's reflex were depicted: stable, relatively stable and disseminated. The stable type prevaled on the right foot as compared to the left one and in males as compared to females. The disseminated type was more frequently seen in females on the left side. In determing the time of Achille's reflex it is necessary to use the arithmetical mean not less than 15--20 measurements.", "PMID": 961297}
{"id": "pubmed23n0032_4301", "title": "[Neuron-capillary interrelationships in the lateral horn of the spinal cord].", "content": "The authors depicted some regularities in the interrelationships of capillaries and different types of neurons in the lateral horns of the spinal cord in rats. It was established that with an increase of the area of the profile field of the nervous cell there is an increase of the amount of capillaries, their total length and the area of the capillary channel in the zone of vascularization. However, the index of capillary-neuron relationships reveals a reverse dependency. The greatest amount of capillaries is stituated in the radius from 5--15 mum from the cell body. The capillaries form around the cell body structures of a different configuration.", "contents": "[Neuron-capillary interrelationships in the lateral horn of the spinal cord]. The authors depicted some regularities in the interrelationships of capillaries and different types of neurons in the lateral horns of the spinal cord in rats. It was established that with an increase of the area of the profile field of the nervous cell there is an increase of the amount of capillaries, their total length and the area of the capillary channel in the zone of vascularization. However, the index of capillary-neuron relationships reveals a reverse dependency. The greatest amount of capillaries is stituated in the radius from 5--15 mum from the cell body. The capillaries form around the cell body structures of a different configuration.", "PMID": 961298}
{"id": "pubmed23n0032_4302", "title": "[Determination of the gliotoxie and demyelinating properties of serum in organ cultures of brain in experimental allergic encephalomyelitis].", "content": "The report contains some results of a study of the blood serum taken from guinea pigs and macacus rhesus with allergic encephalomyelitis to a glyotoxic and demyelinizing effect in an organ culture of the brain in newborn rats and adult macacus rhesus. It was demonstrated that the test has a high specificity to a glyotoxic effect of the blood serum in experimental allergic encephalomyelitis and a less specificity to demyelinization in vitro. The authors describe in detail the method of studying the serum in organ brain cultures.", "contents": "[Determination of the gliotoxie and demyelinating properties of serum in organ cultures of brain in experimental allergic encephalomyelitis]. The report contains some results of a study of the blood serum taken from guinea pigs and macacus rhesus with allergic encephalomyelitis to a glyotoxic and demyelinizing effect in an organ culture of the brain in newborn rats and adult macacus rhesus. It was demonstrated that the test has a high specificity to a glyotoxic effect of the blood serum in experimental allergic encephalomyelitis and a less specificity to demyelinization in vitro. The authors describe in detail the method of studying the serum in organ brain cultures.", "PMID": 961299}
{"id": "pubmed23n0032_4303", "title": "[Development of synaptic contacts in the human brain during the early stages of embryogenesis].", "content": "The author studied the ultrastructure of forming synaptical contacts in the human brain at the early stages of development (7--8 and 9--11 week embryos). It was established that during the 7--8th week of the embryo ontogenesis the brain has already contacts of the puncta adhaerentia type and structures which by several signs can referred to transitory stages in the formation of synapses. During this period of development contacts between the axons of neuroblasts were encountered more frequently that those between the axons and bodies of nervous cells. During the 9--11th weeks of embryogenesis the human brain acquires more contacts between both the axons and the neuroblasts. At this stage there is a first appearance of synaptical structures, similar to functionally and morphologically mature synapses.", "contents": "[Development of synaptic contacts in the human brain during the early stages of embryogenesis]. The author studied the ultrastructure of forming synaptical contacts in the human brain at the early stages of development (7--8 and 9--11 week embryos). It was established that during the 7--8th week of the embryo ontogenesis the brain has already contacts of the puncta adhaerentia type and structures which by several signs can referred to transitory stages in the formation of synapses. During this period of development contacts between the axons of neuroblasts were encountered more frequently that those between the axons and bodies of nervous cells. During the 9--11th weeks of embryogenesis the human brain acquires more contacts between both the axons and the neuroblasts. At this stage there is a first appearance of synaptical structures, similar to functionally and morphologically mature synapses.", "PMID": 961300}
{"id": "pubmed23n0032_4304", "title": "[Changes in the neurons of the lateral geniculate body under the effect of prolonged darkness (cytochemical study)].", "content": "With the aid of the cytospectrophotometry method the author studied the influence of light deprivation (the exposure of animals to darkness during 8 weeks following birth) on neurons of the dorsal nucleus of the external geniculate body of rats according to the index of glutamatedehydrogenase activity. It was established that unlike the visual cortex, separate groups of neurons which react differently to light deprivation, the majority of nervous cells in the external geniculate body demonstrate similar changes: a drop in the medium activity of glutamatdehydrogenase in neurons and its neurological satellites, a shift of neuron histograms according to this index to the left. These changes are accompanied by an increase of smaller neurons as compared to normal, as well as a drop in the amount of neurons with satellites. The found differences of reactions to light deprivation between the cortical neurons and switch areas of the visual analyzer are discussed in the light of functional traits of such neurons.", "contents": "[Changes in the neurons of the lateral geniculate body under the effect of prolonged darkness (cytochemical study)]. With the aid of the cytospectrophotometry method the author studied the influence of light deprivation (the exposure of animals to darkness during 8 weeks following birth) on neurons of the dorsal nucleus of the external geniculate body of rats according to the index of glutamatedehydrogenase activity. It was established that unlike the visual cortex, separate groups of neurons which react differently to light deprivation, the majority of nervous cells in the external geniculate body demonstrate similar changes: a drop in the medium activity of glutamatdehydrogenase in neurons and its neurological satellites, a shift of neuron histograms according to this index to the left. These changes are accompanied by an increase of smaller neurons as compared to normal, as well as a drop in the amount of neurons with satellites. The found differences of reactions to light deprivation between the cortical neurons and switch areas of the visual analyzer are discussed in the light of functional traits of such neurons.", "PMID": 961302}
{"id": "pubmed23n0032_4305", "title": "[Cytochemical indices of intercentral interrelations of the motor and visual analyzers during light deprivation].", "content": "The authors used the interferometrical method to determine the content and concentration of the proteins in the neuron cytoplasma of the III and V layer in the motor and visual areas of the brain cortex in rabbits in normal conditions and prolonged light deprivation (from the moment of birth up to 2 1/2 months). These data were accompanied by measurements of the neuron area. It was demonstrated that the absence of visual impulses leads to a morphochemical underdevelopment of the neurons in the visual cortex layers. Such underdevelopment was expressed in a decrease in the size of the neurons and protein content in the cytoplasma compared to the control group. In the neurons of the III and V layer of the motor cortex of the same animals there were changes of a compensatory character (an increase in the size of the neurons, content and concentration of proteins in the cytoplasma). In this respect the authors discuss the question of the influence of specific visual and other forms of impulsation on the morphochemical differentiation of neurons in the studied brain cortex.", "contents": "[Cytochemical indices of intercentral interrelations of the motor and visual analyzers during light deprivation]. The authors used the interferometrical method to determine the content and concentration of the proteins in the neuron cytoplasma of the III and V layer in the motor and visual areas of the brain cortex in rabbits in normal conditions and prolonged light deprivation (from the moment of birth up to 2 1/2 months). These data were accompanied by measurements of the neuron area. It was demonstrated that the absence of visual impulses leads to a morphochemical underdevelopment of the neurons in the visual cortex layers. Such underdevelopment was expressed in a decrease in the size of the neurons and protein content in the cytoplasma compared to the control group. In the neurons of the III and V layer of the motor cortex of the same animals there were changes of a compensatory character (an increase in the size of the neurons, content and concentration of proteins in the cytoplasma). In this respect the authors discuss the question of the influence of specific visual and other forms of impulsation on the morphochemical differentiation of neurons in the studied brain cortex.", "PMID": 961303}
{"id": "pubmed23n0032_4306", "title": "Effect of adrenal demedullation on urinary excretion of catecholamines in thermal trauma in rats.", "content": "The urinary excretions of adrenaline and noradrenaline were determined before and after a moderate thermal trauma in rats with intact adrenal glands, after adrenal demedullation and after adrenalectomy with grafting of adrenal cortical tissue. The basal excretions of adrenaline were 0.28 mug, 0.06 mug and 0.04 mug/24 h/rat, respectively. In rats with intact adrenal glands the adrenaline excretion was greatly increased already in the first 24 hours after the burn. In both the demedullated and adrenalectomized rats only a very slight increase of the adrenaline excretion was noted, but this may indicate a slight reaction of the extramedullary chromaffin tissue to the trauma. The basal excretion of noradrenaline was not affected either by demedullation or adrenalectomy, indicating that it consisted mainly in noradrenaline released from peripheral sympathetic nerve endings. From the second day after the burn there was an increase in the noradrenaline excretion, of equal magnitude in all experimental groups, indicating an increased liberation of peripheral adrenergic transmittor substances.", "contents": "Effect of adrenal demedullation on urinary excretion of catecholamines in thermal trauma in rats. The urinary excretions of adrenaline and noradrenaline were determined before and after a moderate thermal trauma in rats with intact adrenal glands, after adrenal demedullation and after adrenalectomy with grafting of adrenal cortical tissue. The basal excretions of adrenaline were 0.28 mug, 0.06 mug and 0.04 mug/24 h/rat, respectively. In rats with intact adrenal glands the adrenaline excretion was greatly increased already in the first 24 hours after the burn. In both the demedullated and adrenalectomized rats only a very slight increase of the adrenaline excretion was noted, but this may indicate a slight reaction of the extramedullary chromaffin tissue to the trauma. The basal excretion of noradrenaline was not affected either by demedullation or adrenalectomy, indicating that it consisted mainly in noradrenaline released from peripheral sympathetic nerve endings. From the second day after the burn there was an increase in the noradrenaline excretion, of equal magnitude in all experimental groups, indicating an increased liberation of peripheral adrenergic transmittor substances.", "PMID": 961316}
{"id": "pubmed23n0032_4307", "title": "The strength of suture knots after one week in vivo.", "content": "The strength of knotted thread for 12 different types of suture knot in combination with 11 types of suture material used in surgery was investigated one week after insertion in subcutaneous tissue in rabbits. The results were compared with similar investigations on knot strength carried out with 'dry knots'. The thread dimension used in both experiments was 000 (USP). As in vitro, the weakest point of a suture loop subjected to traction was the knot. The incidence of thread rupture within the loop but outside the knot was 1.4% in vitro and 5.9% in vivo, and was commonest with plain catgut. With one exception, however, thread breakage occurred at greater strain than the average required to produce knot failure with the material in question. A greater proportion of knots slipped in vivo then in vitro (72% and 35% respectively), with reduction of the strength of the suture loop as result. There was also greater scatter of measured values under in vivo conditions (standard deviation: 11% in vitro, 23% in vivo). For 7 of 11 suture materials the results indicated a reduction in knot-holding power after one week in situ in the living animal compared with in vivo conditions. This was naturally enough most marked with the two types of catgut (chrome 48%, plain 57%). In contrast, Dexon apparently remained unchanged after one week in vivo. The non-absorbable materials tested showed an estimated average fall in knot-holding power from 100% to 88% (range 109-65%), viz, an average of 12% units. The loss of holding power in vivo was, however, apparently never greater than 1/3 compared with 'dry knots' and calculated on average for all types of knot with non-absorbable materials.", "contents": "The strength of suture knots after one week in vivo. The strength of knotted thread for 12 different types of suture knot in combination with 11 types of suture material used in surgery was investigated one week after insertion in subcutaneous tissue in rabbits. The results were compared with similar investigations on knot strength carried out with 'dry knots'. The thread dimension used in both experiments was 000 (USP). As in vitro, the weakest point of a suture loop subjected to traction was the knot. The incidence of thread rupture within the loop but outside the knot was 1.4% in vitro and 5.9% in vivo, and was commonest with plain catgut. With one exception, however, thread breakage occurred at greater strain than the average required to produce knot failure with the material in question. A greater proportion of knots slipped in vivo then in vitro (72% and 35% respectively), with reduction of the strength of the suture loop as result. There was also greater scatter of measured values under in vivo conditions (standard deviation: 11% in vitro, 23% in vivo). For 7 of 11 suture materials the results indicated a reduction in knot-holding power after one week in situ in the living animal compared with in vivo conditions. This was naturally enough most marked with the two types of catgut (chrome 48%, plain 57%). In contrast, Dexon apparently remained unchanged after one week in vivo. The non-absorbable materials tested showed an estimated average fall in knot-holding power from 100% to 88% (range 109-65%), viz, an average of 12% units. The loss of holding power in vivo was, however, apparently never greater than 1/3 compared with 'dry knots' and calculated on average for all types of knot with non-absorbable materials.", "PMID": 961317}
{"id": "pubmed23n0032_4308", "title": "The strength of tissue against individual sutures in structures involved in the repair of inguinal hernia.", "content": "The strength of tissue against individual sutures in structures used in the repair of inguinal hernia was tested in situ in 19 cadavers. Altogether 465 measurements were made. Three different layers were investigated, and the mean tissue strength to a single suture was recorded as the mean of all means for each preparation and aspect as follows. (1) The superficial layer (external oblique aponeurosis). Superior aspect 5.3 kp, inferior aspect 5.5 kp. (2) Middle layer. Superior aspect (internal abdominal oblique), 5.9 kp; inferior aspect (inferior border of the inguinal ligament), 6.8 kp. (3) Deep layer. Superior aspect (transversalis fascia), 5.3 kp; inferior aspect (ilio-pubic tract), 6.9 kp. In Cooper's ligament the mean holding power of the tissue against a single suture was found to be 9.1 kp. The site of the most medial of the sutures in the superior aspect of the middle layer corresponds to the conjoined tendon, and the mean tissue strength to this suture was 11.0 kp. The findings thus suggest that the strengths of the tissues of the anterior and posterior walls of the inguinal canal are roughly equal. The structures making up the conjoined tendon and Cooper's ligament were strongest, however, and showed tissue strengths nearly twice those of other structures.", "contents": "The strength of tissue against individual sutures in structures involved in the repair of inguinal hernia. The strength of tissue against individual sutures in structures used in the repair of inguinal hernia was tested in situ in 19 cadavers. Altogether 465 measurements were made. Three different layers were investigated, and the mean tissue strength to a single suture was recorded as the mean of all means for each preparation and aspect as follows. (1) The superficial layer (external oblique aponeurosis). Superior aspect 5.3 kp, inferior aspect 5.5 kp. (2) Middle layer. Superior aspect (internal abdominal oblique), 5.9 kp; inferior aspect (inferior border of the inguinal ligament), 6.8 kp. (3) Deep layer. Superior aspect (transversalis fascia), 5.3 kp; inferior aspect (ilio-pubic tract), 6.9 kp. In Cooper's ligament the mean holding power of the tissue against a single suture was found to be 9.1 kp. The site of the most medial of the sutures in the superior aspect of the middle layer corresponds to the conjoined tendon, and the mean tissue strength to this suture was 11.0 kp. The findings thus suggest that the strengths of the tissues of the anterior and posterior walls of the inguinal canal are roughly equal. The structures making up the conjoined tendon and Cooper's ligament were strongest, however, and showed tissue strengths nearly twice those of other structures.", "PMID": 961318}
{"id": "pubmed23n0032_4309", "title": "Abdominal angina. Results of arterial reconstruction in 12 patients.", "content": "Twelve patients suffering from abdominal angina have been operated upon with revascularization of the superior mesenteric artery. Preoperatively, all had the classical symptoms: postprandial pain, wieght loss and abnormalities of the stools. The surgical procedure of choice was endarterectomy with patch grafting; in two cases a vein by-pass have been used. In general, it will be sufficient to reconstruct one artery, even with two or three of the mesenteric arteries afflicted. Peroperative measurements of pressure gradients and flow may guide in choice of procedure. Two patients died postoperatively, both had additional extensive reconstruction of the aorta and the renal arteries. The remaining 10 patients were all relieved of their symptoms after the operation and gained considerably in weight. They were followed over 25 months in mean. During the period of observation 2 patients have died from other diseases. The good longterm results after arterial reconstruction in contrast to the poor prognosis without operation call for early diagnosis and surgical treatment. The difficulties involved with the diagnosis of abdominal angina are discussed and a functional diagnostic test is proposed.", "contents": "Abdominal angina. Results of arterial reconstruction in 12 patients. Twelve patients suffering from abdominal angina have been operated upon with revascularization of the superior mesenteric artery. Preoperatively, all had the classical symptoms: postprandial pain, wieght loss and abnormalities of the stools. The surgical procedure of choice was endarterectomy with patch grafting; in two cases a vein by-pass have been used. In general, it will be sufficient to reconstruct one artery, even with two or three of the mesenteric arteries afflicted. Peroperative measurements of pressure gradients and flow may guide in choice of procedure. Two patients died postoperatively, both had additional extensive reconstruction of the aorta and the renal arteries. The remaining 10 patients were all relieved of their symptoms after the operation and gained considerably in weight. They were followed over 25 months in mean. During the period of observation 2 patients have died from other diseases. The good longterm results after arterial reconstruction in contrast to the poor prognosis without operation call for early diagnosis and surgical treatment. The difficulties involved with the diagnosis of abdominal angina are discussed and a functional diagnostic test is proposed.", "PMID": 961319}
{"id": "pubmed23n0032_4310", "title": "Fibrinogen related antigens in acute pancreatitis.", "content": "The fibrinogen related antigens were measured in 22 consecutive patients with acute pancreatitis. During the attack a marked elevation was seen, the values normalizing in the quiet phase. This may be due to either intravascular and/or extravascular degradation of fibrinogen-fibrin. As other authors have demonstrated intravascular coagulation experimentally in animals with pancreatitis, the authors stress that intravascular coagulation may play an important role in the patheogenesis of acute pancreatitis.", "contents": "Fibrinogen related antigens in acute pancreatitis. The fibrinogen related antigens were measured in 22 consecutive patients with acute pancreatitis. During the attack a marked elevation was seen, the values normalizing in the quiet phase. This may be due to either intravascular and/or extravascular degradation of fibrinogen-fibrin. As other authors have demonstrated intravascular coagulation experimentally in animals with pancreatitis, the authors stress that intravascular coagulation may play an important role in the patheogenesis of acute pancreatitis.", "PMID": 961320}
{"id": "pubmed23n0032_4311", "title": "Blood flow and oxygen consumption in the feline small intestine; responses to artificial distension and intestinal obstruction.", "content": "Relations between blood flow and oxygen consumption were studied in denervated and homologously perfused small intestine of the cat. Under \"resting\" conditions, oxygen uptake was independent of blood flow within rather wide limits, whereas fractional oxygen extraction displayed a highly significant correlation to flow. Upon artificial distension of the bowel, oxygen uptake became a function of blood flow. It is suggested that there may exist an \"autoregulation\" of oxygen uptake, striving to satisfy theoxygen need of the bowel irrespective of the current blood-flow rate. It is also suggested that this \"autoregulation\" is abolished upon distension of the bowel. With respect to intestinal obstruction, the continuing distension of simple obstruction causes an inability of the bowell-wall vasculature to extract and consume the oxygen currently needed, probably a result of disturbed \"autoregulation\" of oxygen uptake. An intervening decompression seems to re-establish \"autoregulation\" in the obstructed bowel. The present results afford evidence for \"autoregulation\" of intestinal oxygen uptake. They further suggest deterioration of \"autoregulation\" in intestinal obstruction and corroborate the previous suggestion that intra-operative decompression of obstructed bowel restores the ability of its capillary circulation to withstand with retained function, renewed increments of strain and distension.", "contents": "Blood flow and oxygen consumption in the feline small intestine; responses to artificial distension and intestinal obstruction. Relations between blood flow and oxygen consumption were studied in denervated and homologously perfused small intestine of the cat. Under \"resting\" conditions, oxygen uptake was independent of blood flow within rather wide limits, whereas fractional oxygen extraction displayed a highly significant correlation to flow. Upon artificial distension of the bowel, oxygen uptake became a function of blood flow. It is suggested that there may exist an \"autoregulation\" of oxygen uptake, striving to satisfy theoxygen need of the bowel irrespective of the current blood-flow rate. It is also suggested that this \"autoregulation\" is abolished upon distension of the bowel. With respect to intestinal obstruction, the continuing distension of simple obstruction causes an inability of the bowell-wall vasculature to extract and consume the oxygen currently needed, probably a result of disturbed \"autoregulation\" of oxygen uptake. An intervening decompression seems to re-establish \"autoregulation\" in the obstructed bowel. The present results afford evidence for \"autoregulation\" of intestinal oxygen uptake. They further suggest deterioration of \"autoregulation\" in intestinal obstruction and corroborate the previous suggestion that intra-operative decompression of obstructed bowel restores the ability of its capillary circulation to withstand with retained function, renewed increments of strain and distension.", "PMID": 961321}
{"id": "pubmed23n0032_4312", "title": "Ruptured arteriosclerotic aneurysm of the splenic artery.", "content": "A case of arteriosclerotic aneurysm of the splenic artery with rupture into the transverse colon resulting in secondary perforation of the cecum is presented. Symptomatology, diagnosis and treatment of aneurysms of the splenic artery are briefly illustrated. Aneurysm of the splenic artery must be suspected by the presence of uncharacteristic upper abdominal pain and the annular calcification in the left upper quadrant on roentgenogram. The diagnosis is verified by arteriography. Operation of all symptomatic splenic artery aneurysms is recommended. Asymptomatic aneurysms measuring more than 3 cm in diameter and aneurysms with radiographic evidence of enlargement, should be operated too.", "contents": "Ruptured arteriosclerotic aneurysm of the splenic artery. A case of arteriosclerotic aneurysm of the splenic artery with rupture into the transverse colon resulting in secondary perforation of the cecum is presented. Symptomatology, diagnosis and treatment of aneurysms of the splenic artery are briefly illustrated. Aneurysm of the splenic artery must be suspected by the presence of uncharacteristic upper abdominal pain and the annular calcification in the left upper quadrant on roentgenogram. The diagnosis is verified by arteriography. Operation of all symptomatic splenic artery aneurysms is recommended. Asymptomatic aneurysms measuring more than 3 cm in diameter and aneurysms with radiographic evidence of enlargement, should be operated too.", "PMID": 961322}
{"id": "pubmed23n0032_4313", "title": "Acute segmental gastro-intestinal gangrene probably of clostridial origin. Report of a case.", "content": "A 29-year-old female suffering from acute terminal ileitis was subjected to laparotomy undertaken on the tentative diagnosis of acute appendicitis. The appendix was found to be normal and was removed. The course was complicated with segmental gastro-intestinal gangrene with fatal outcome. The involvement of histotoxic clostridial infection as a causative factor is discussed.", "contents": "Acute segmental gastro-intestinal gangrene probably of clostridial origin. Report of a case. A 29-year-old female suffering from acute terminal ileitis was subjected to laparotomy undertaken on the tentative diagnosis of acute appendicitis. The appendix was found to be normal and was removed. The course was complicated with segmental gastro-intestinal gangrene with fatal outcome. The involvement of histotoxic clostridial infection as a causative factor is discussed.", "PMID": 961323}
{"id": "pubmed23n0032_4314", "title": "Effect of cadmium chloride on testis and seminal vesicular fructose contents of shrew (Suncus murinus anderson), gerbil (Meriones hurrianae jerdon) and hedgehog (Hemiechinus auritus collaris).", "content": "A single subcutaneous injection of cadmium chloride (0.04 m mol/kg body weight) did not cause any change in the macroscopic and microscopic features of testes and caput epididymis of Suncus. Testes and sex accessory glands did not show any significant change in their weights. Seminal vesicular fructose concentration remains unchanged. The tetes and sex accessory organs of gerbil under comparable condition showed significant weight loss and shrinkage in tubule diameter. The seminiferous epithelium was totally destroyed. A single intratesticular injection of CdCl2 (0.5 mg/kg body weight) in hedgehog resulted in testicular necrosis. The small temperature difference between cremaster sac and rectum, absence of testicular artery and pampiniform plexus and insuficient concentration of cadmium within the testis when administered subcutaneously may be responsible for insensitivity of Suncus testes to cadmium.", "contents": "Effect of cadmium chloride on testis and seminal vesicular fructose contents of shrew (Suncus murinus anderson), gerbil (Meriones hurrianae jerdon) and hedgehog (Hemiechinus auritus collaris). A single subcutaneous injection of cadmium chloride (0.04 m mol/kg body weight) did not cause any change in the macroscopic and microscopic features of testes and caput epididymis of Suncus. Testes and sex accessory glands did not show any significant change in their weights. Seminal vesicular fructose concentration remains unchanged. The tetes and sex accessory organs of gerbil under comparable condition showed significant weight loss and shrinkage in tubule diameter. The seminiferous epithelium was totally destroyed. A single intratesticular injection of CdCl2 (0.5 mg/kg body weight) in hedgehog resulted in testicular necrosis. The small temperature difference between cremaster sac and rectum, absence of testicular artery and pampiniform plexus and insuficient concentration of cadmium within the testis when administered subcutaneously may be responsible for insensitivity of Suncus testes to cadmium.", "PMID": 961326}
{"id": "pubmed23n0032_4315", "title": "[Management of cervical incompetence with cerclage according to Shirodkar. A report on 110 cases].", "content": "The authors report a 110 cervical cerclage operations for cervical incompetence. Shirodkar's technique was used and cerclage was carried out between the 9th and 20th week of amenorrhea. Out of 110 patients submitted to the procedure, 98 gave birth to a live infant weighing more than 1000 g which amounts to a success rate of 89%. Duration of pregnancy ranged between 26 and 41 weeks. If only pregnancies having proceeded to 37 weeks of amenorrhea are considered, the success rate amounts to 68%.", "contents": "[Management of cervical incompetence with cerclage according to Shirodkar. A report on 110 cases]. The authors report a 110 cervical cerclage operations for cervical incompetence. Shirodkar's technique was used and cerclage was carried out between the 9th and 20th week of amenorrhea. Out of 110 patients submitted to the procedure, 98 gave birth to a live infant weighing more than 1000 g which amounts to a success rate of 89%. Duration of pregnancy ranged between 26 and 41 weeks. If only pregnancies having proceeded to 37 weeks of amenorrhea are considered, the success rate amounts to 68%.", "PMID": 961327}
{"id": "pubmed23n0032_4316", "title": "Hemodynamic drug interaction: peridural lidocaine and intravenous ephedrine.", "content": "Hypotension following peridural anesthesia with lidocaine was treated by intravenous injection of ephedrine. The ephedrine relieved the cardiovascular depression, but was associated with a concomitant increase in plasma lidocaine concentrations. This increase may push the plasma lidocaine concentration into the toxic region.", "contents": "Hemodynamic drug interaction: peridural lidocaine and intravenous ephedrine. Hypotension following peridural anesthesia with lidocaine was treated by intravenous injection of ephedrine. The ephedrine relieved the cardiovascular depression, but was associated with a concomitant increase in plasma lidocaine concentrations. This increase may push the plasma lidocaine concentration into the toxic region.", "PMID": 961328}
{"id": "pubmed23n0032_4317", "title": "Suxamethonium apnoea terminated with commercial serumcholinesterase.", "content": "A case of prolonged suxamethonium apnoea successfully terminated by the infusion of a commercial preparation of serumcholinesterase is reported. The patient appeared to be homozygous for the dibucaine resistant gene, having only 15% of normal activity in his serum. His dibucaine number was 21, and the Michaelis constant was 5.5 times that of normal sera. One and a half hours after receiving 110 mg suxamethonium for oesophagoscopy, the patient was still apnoeic with no response to ulnar nerve stimulation. Intravenous administration of 90 mg commercial serumcholinesterase, the equivalent to 1000 ml fresh human plasma, restored twitch and tetanic responses and the patient could lift his head 15 min after the beginning of the enzyme injection. The serumcholinesterase activity of the patient's serum increased by 55% (from 15% to 70%) following the injection. This rise was halved over the next 8 days.", "contents": "Suxamethonium apnoea terminated with commercial serumcholinesterase. A case of prolonged suxamethonium apnoea successfully terminated by the infusion of a commercial preparation of serumcholinesterase is reported. The patient appeared to be homozygous for the dibucaine resistant gene, having only 15% of normal activity in his serum. His dibucaine number was 21, and the Michaelis constant was 5.5 times that of normal sera. One and a half hours after receiving 110 mg suxamethonium for oesophagoscopy, the patient was still apnoeic with no response to ulnar nerve stimulation. Intravenous administration of 90 mg commercial serumcholinesterase, the equivalent to 1000 ml fresh human plasma, restored twitch and tetanic responses and the patient could lift his head 15 min after the beginning of the enzyme injection. The serumcholinesterase activity of the patient's serum increased by 55% (from 15% to 70%) following the injection. This rise was halved over the next 8 days.", "PMID": 961329}
{"id": "pubmed23n0032_4318", "title": "Effect of ketamine anaesthesia on the content of monoamines and their metabolites in the rat brain.", "content": "The effects of ketamine anaesthesia (100 mg/kg i.p.) on the content of brain 5-hydroxytryptamine (5HT), 5-hydroxyindoleacetic acid (5HIAA), noradrenaline (NA), dopamine (DA) and homovanillic acid (HVA) were studied in male Wistar rats. Fifteen min after ketamine injection, when the rats were deeply anaesthetized, the 5HT content in many brain regions tended to be increased. An opposite tendency was found in the brain 5HIAA content. In rats treated with probenecid, which markedly lengthened ketamine anaesthesia, the accumulation of 5HIAA was significantly reduced by ketamine. In addition to ketamine anaesthesia, probenecid was found to lengthen thiopental anaesthesia. One hour after the ketamine administration, when the rats were no longer anaesthetized but were excited, the brain NA concentration was increased by 17% (P less than 0.02). The brain DA content was unchanged, but at 15 min and 1 hour after ketamine administration the striatal HVA content was increased by about 55% (P less than 0.05), suggesting an increased turnover of DA. The results suggest that during recovery from ketamine anaesthesia the increased NA content and the increased DA turnover may be associated with the postanaesthetic excitement of the rat, whereas the decreasamine anaesthesia.", "contents": "Effect of ketamine anaesthesia on the content of monoamines and their metabolites in the rat brain. The effects of ketamine anaesthesia (100 mg/kg i.p.) on the content of brain 5-hydroxytryptamine (5HT), 5-hydroxyindoleacetic acid (5HIAA), noradrenaline (NA), dopamine (DA) and homovanillic acid (HVA) were studied in male Wistar rats. Fifteen min after ketamine injection, when the rats were deeply anaesthetized, the 5HT content in many brain regions tended to be increased. An opposite tendency was found in the brain 5HIAA content. In rats treated with probenecid, which markedly lengthened ketamine anaesthesia, the accumulation of 5HIAA was significantly reduced by ketamine. In addition to ketamine anaesthesia, probenecid was found to lengthen thiopental anaesthesia. One hour after the ketamine administration, when the rats were no longer anaesthetized but were excited, the brain NA concentration was increased by 17% (P less than 0.02). The brain DA content was unchanged, but at 15 min and 1 hour after ketamine administration the striatal HVA content was increased by about 55% (P less than 0.05), suggesting an increased turnover of DA. The results suggest that during recovery from ketamine anaesthesia the increased NA content and the increased DA turnover may be associated with the postanaesthetic excitement of the rat, whereas the decreasamine anaesthesia.", "PMID": 961330}
{"id": "pubmed23n0032_4319", "title": "Respiration and circulation after total hip replacement surgery. A comparison between parenteral analgesics and continuous lumbar epidural block.", "content": "The cardiopulmonary effects of two different types of postoperative analgesic regimes were compared in 31 cardiorespiratorily healthy patients subjected to total hip replacement surgery. The investigation was performed preoperatively on the morning of the day of surgery and during the first 3 days postoperatively. All patients received continuous lumbar epidural analgesia preoperatively, during surgery and up to the end of the first measurement period, which started 2.5 h after surgery. Ten patients were subseuqently given pentazocine (Fortalgesic) intramuscularly on demand for pain relief throughout the investigation, while 14 patients received 0.4% plain lidocaine (Xylocain), and seven patients 0.4% lidocaine with adrenaline (1/400,000) as a continuous lumbar epidural drip for analgesia thorughout the investigation. It was confirmed that the operative procedure itself did not significantly influence the postoperative arterial oxygenation, while the type of postoperative analgesic regimen was of considerable importance in this respect. Thus, patients given pentazocine showed a significant increase in pulmonary venous admixture, due both to an increase in true shunt and to an increase in ventilation/perfusion disturbances. This pattern of poor pulmonary function still persisted on the third postoperatively. In patients given an epidural block no significant changes in pulmonary venous admixture were noted postoperatively, and thus there was no reduction in PaO2. All patients, irrespective of the type of analgesic regimen used, had a significantly increased cardiac index and oxygen uptake postoperatively, although patients given an epidural block showed a greater increase in cardiac index, and thus a tendency towards a more hyperkinetic circulation than those given pentazocine.", "contents": "Respiration and circulation after total hip replacement surgery. A comparison between parenteral analgesics and continuous lumbar epidural block. The cardiopulmonary effects of two different types of postoperative analgesic regimes were compared in 31 cardiorespiratorily healthy patients subjected to total hip replacement surgery. The investigation was performed preoperatively on the morning of the day of surgery and during the first 3 days postoperatively. All patients received continuous lumbar epidural analgesia preoperatively, during surgery and up to the end of the first measurement period, which started 2.5 h after surgery. Ten patients were subseuqently given pentazocine (Fortalgesic) intramuscularly on demand for pain relief throughout the investigation, while 14 patients received 0.4% plain lidocaine (Xylocain), and seven patients 0.4% lidocaine with adrenaline (1/400,000) as a continuous lumbar epidural drip for analgesia thorughout the investigation. It was confirmed that the operative procedure itself did not significantly influence the postoperative arterial oxygenation, while the type of postoperative analgesic regimen was of considerable importance in this respect. Thus, patients given pentazocine showed a significant increase in pulmonary venous admixture, due both to an increase in true shunt and to an increase in ventilation/perfusion disturbances. This pattern of poor pulmonary function still persisted on the third postoperatively. In patients given an epidural block no significant changes in pulmonary venous admixture were noted postoperatively, and thus there was no reduction in PaO2. All patients, irrespective of the type of analgesic regimen used, had a significantly increased cardiac index and oxygen uptake postoperatively, although patients given an epidural block showed a greater increase in cardiac index, and thus a tendency towards a more hyperkinetic circulation than those given pentazocine.", "PMID": 961331}
{"id": "pubmed23n0032_4320", "title": "Misplacement and loop formation of central venous catheters.", "content": "Exact placement is an essential prerequisite for long-term use of a central venous catheter. Reported data show an extremely wide range of catheteral misplacements: from less than 1% to more than 60%. Some approaches appear to be less advantageous than others, but the highest rates of misplacement occur in the cubital, external jugular and saphenous veins. A series is presented of 378 radiographically controlled central venous catheters analysed for aberrant placement and loop formation. The total occurrence of faulty positioning and coiling reached 5.3%, while the respective incidences were 30% for the external jugular vein, 5.7% for the internal jugular vein, 5.5% for the infraclavicular technique of subclavian venepuncture, 5.3% for the innominate vein and 1.4% for the supraclavicular approach of subclavian venepuncture. The total frequency for pure loop formation was 2.9%. The authors discuss numerous reported data on catheter malpositioning, according to the specific techniques used, and compare them with thier own results. The relatively low incidence in the present series is possibly due to the high proportion of cases where the supraclavicular subclavian approach was used, the omission of the sphrenous/femoral and cubital techniques, and to pre-determining the length of the inserted catheteral segments.", "contents": "Misplacement and loop formation of central venous catheters. Exact placement is an essential prerequisite for long-term use of a central venous catheter. Reported data show an extremely wide range of catheteral misplacements: from less than 1% to more than 60%. Some approaches appear to be less advantageous than others, but the highest rates of misplacement occur in the cubital, external jugular and saphenous veins. A series is presented of 378 radiographically controlled central venous catheters analysed for aberrant placement and loop formation. The total occurrence of faulty positioning and coiling reached 5.3%, while the respective incidences were 30% for the external jugular vein, 5.7% for the internal jugular vein, 5.5% for the infraclavicular technique of subclavian venepuncture, 5.3% for the innominate vein and 1.4% for the supraclavicular approach of subclavian venepuncture. The total frequency for pure loop formation was 2.9%. The authors discuss numerous reported data on catheter malpositioning, according to the specific techniques used, and compare them with thier own results. The relatively low incidence in the present series is possibly due to the high proportion of cases where the supraclavicular subclavian approach was used, the omission of the sphrenous/femoral and cubital techniques, and to pre-determining the length of the inserted catheteral segments.", "PMID": 961332}
{"id": "pubmed23n0032_4321", "title": "Postanaesthetic use of naloxone hydrochloride after moderate doses of fentanyl.", "content": "A consecutive series of 273 anaesthetics is presented. All patients were given moderate doses of droperidol and fentanyl intravenously to supplement nitrous oxide and oxygen anaesthesia. 28.6% of the patients needed reversal of the analgesic respiratory depressant effect at the end of anaesthesia to establish stable spontaneous respiration. The opiate antagonist, naloxone hydrochloride (Narcan), was found to give rapid and reliable reversal of the respiratory depression. A mean dose of 2 mug/kg body weight of naloxone was found adequate in that no patient required further doses in the post-operative period in order to maintain adequate ventilation. Neither does the dose seem to have been too large. Patients in the naloxone group had no need for additional analgesics during the first 5 3/4 hours postoperatively, as compared to the painfree interval of 3 1/4 hours in the control group.", "contents": "Postanaesthetic use of naloxone hydrochloride after moderate doses of fentanyl. A consecutive series of 273 anaesthetics is presented. All patients were given moderate doses of droperidol and fentanyl intravenously to supplement nitrous oxide and oxygen anaesthesia. 28.6% of the patients needed reversal of the analgesic respiratory depressant effect at the end of anaesthesia to establish stable spontaneous respiration. The opiate antagonist, naloxone hydrochloride (Narcan), was found to give rapid and reliable reversal of the respiratory depression. A mean dose of 2 mug/kg body weight of naloxone was found adequate in that no patient required further doses in the post-operative period in order to maintain adequate ventilation. Neither does the dose seem to have been too large. Patients in the naloxone group had no need for additional analgesics during the first 5 3/4 hours postoperatively, as compared to the painfree interval of 3 1/4 hours in the control group.", "PMID": 961333}
{"id": "pubmed23n0032_4322", "title": "The effect of segmental epidural analgesia on maternal and foetal acid-base balance, lactate, serum potassium and creatine phosphokinase during labour.", "content": "Maternal and foetal acid-base balance, PaO2, lactate, potassium and creatine phosphokinase (CPK) were studied during the course of 28 induced labours. Every second mother received segmental epidural analgesia during the first stage of labour (epidural group), while the remaining mothers (who were given pethidine for pain relief, if necessary) acted as a control group. In the epidural group the patients had only minimal changes in acid-base balance and lactate concentration during the first stage. During the second stage lactate concentration increased. In the control group, on the other hand, the acid-base balance showed signs of hyperventilation and lactic acid accumulation during the first stage. The potassium changes were quite minimal and were not significantly different between the groups. The CPK level did not change during labour, but 2 and 4 h after delivery it was significantly elevated in both groups. The foetal acid-base balance, potassium, lactate and PaO2 values revealed no differences between the groups at any time. The CPK level in umbilical venous blood was significantly higher in the epidural group.", "contents": "The effect of segmental epidural analgesia on maternal and foetal acid-base balance, lactate, serum potassium and creatine phosphokinase during labour. Maternal and foetal acid-base balance, PaO2, lactate, potassium and creatine phosphokinase (CPK) were studied during the course of 28 induced labours. Every second mother received segmental epidural analgesia during the first stage of labour (epidural group), while the remaining mothers (who were given pethidine for pain relief, if necessary) acted as a control group. In the epidural group the patients had only minimal changes in acid-base balance and lactate concentration during the first stage. During the second stage lactate concentration increased. In the control group, on the other hand, the acid-base balance showed signs of hyperventilation and lactic acid accumulation during the first stage. The potassium changes were quite minimal and were not significantly different between the groups. The CPK level did not change during labour, but 2 and 4 h after delivery it was significantly elevated in both groups. The foetal acid-base balance, potassium, lactate and PaO2 values revealed no differences between the groups at any time. The CPK level in umbilical venous blood was significantly higher in the epidural group.", "PMID": 961334}
{"id": "pubmed23n0032_4323", "title": "Mast cells in mammalian brain.", "content": "Mast cells, which had until recently been believed to be not present in the mammalian brain, were studied in the brains of 29 mammalian species. Although there was considerable intraspecific and interspecific variation, mast cells were most numerous within the leptomeninges (especially in those overlying the cerebrum and the dorsal thalamus - most rodents, most carnivores, chimpanzees, squirrel monkeys and elephant), the cerebral cortex (most rodents, tiger, fox, chimpanzee, tarsier, and elephant) and in many nuclei of the dorsal thalamus (most rodents, tiger, lion, and fox). In some mammals, mast cells were also numerous in the stroma of the telencephalic choroid plexuses (chimpanzee, squirrel monkey), the putamen and the claustrum (chimpanzee), the subfornical organ (pack rat, tiger, chimpanzee), the olfactory peduncles (hooded rat, albino rat), the stroma of the diencephalic choroid plexus (lion, chimpanzee, squirrel monkey), the pineal organ (chimpanzee, squirrel monkey), some nuclei of the hypothalamus (tiger), the infundibulum (hooded rat, tiger, fox) the area postrema (pack rat, chinchilla, lion, spider monkey, chimpanzee, fox) and some nuclei and tracts of the metencephalon and the myelencephalon (tiger). Neither the sex of the animal nor electrolytic lesions made in the brains of some of the animals at various times prior to sacrifice appeared to effect the number and the distribution of mast cells. Age-related changes in mast cell number and distribution were detected in the albino rat.", "contents": "Mast cells in mammalian brain. Mast cells, which had until recently been believed to be not present in the mammalian brain, were studied in the brains of 29 mammalian species. Although there was considerable intraspecific and interspecific variation, mast cells were most numerous within the leptomeninges (especially in those overlying the cerebrum and the dorsal thalamus - most rodents, most carnivores, chimpanzees, squirrel monkeys and elephant), the cerebral cortex (most rodents, tiger, fox, chimpanzee, tarsier, and elephant) and in many nuclei of the dorsal thalamus (most rodents, tiger, lion, and fox). In some mammals, mast cells were also numerous in the stroma of the telencephalic choroid plexuses (chimpanzee, squirrel monkey), the putamen and the claustrum (chimpanzee), the subfornical organ (pack rat, tiger, chimpanzee), the olfactory peduncles (hooded rat, albino rat), the stroma of the diencephalic choroid plexus (lion, chimpanzee, squirrel monkey), the pineal organ (chimpanzee, squirrel monkey), some nuclei of the hypothalamus (tiger), the infundibulum (hooded rat, tiger, fox) the area postrema (pack rat, chinchilla, lion, spider monkey, chimpanzee, fox) and some nuclei and tracts of the metencephalon and the myelencephalon (tiger). Neither the sex of the animal nor electrolytic lesions made in the brains of some of the animals at various times prior to sacrifice appeared to effect the number and the distribution of mast cells. Age-related changes in mast cell number and distribution were detected in the albino rat.", "PMID": 961335}
{"id": "pubmed23n0032_4324", "title": "[Morphology, topography and nomenclature of the human papillary muscles compared with mammalian].", "content": "On the internal or parietal surface of the left ventricle in man and in mammals are two papillary muscles, which are almost identical and well developed. In man, these muscles are known as the m. papillaris parietalis anterior sinister and the m. papillaris parietalis posterior dexter, in mammals, the m. papillaris parietalis cranialis sinister and caudalis dexter, or, in shorter form, mm. papillaris parietalis sinister et dexter. In the right ventricle in man, there are two papillary parietal muscles: the mm. papillares anteriores et posteriores. On the septum of this ventricle there is, as in mammals, a muscle called the m. papillaris septalis medialis seu subarteriosus. Beside it are one or several smaller muscles, varying from one individual to another: the mm. papillares septales accessorii seu parvi. In the right ventricle of the mammalian heart is found, in addition to the m. parietalis septalis subarteriosus, already mentioned, a m. papillaris caudalis, more or less well developed in some species and, in the majority of mammals, the m. papillaris septalis cranials, which is always well developed. In certain mammals, there is, in rare cases, a m. papillaris septomarginalis seu parietalis. It may be said, in conclusion, that, in a large number of mammals, there is, on the internal surface of the external wall of the right ventricle, a reasonably well developed m. papillaris parietalis.", "contents": "[Morphology, topography and nomenclature of the human papillary muscles compared with mammalian]. On the internal or parietal surface of the left ventricle in man and in mammals are two papillary muscles, which are almost identical and well developed. In man, these muscles are known as the m. papillaris parietalis anterior sinister and the m. papillaris parietalis posterior dexter, in mammals, the m. papillaris parietalis cranialis sinister and caudalis dexter, or, in shorter form, mm. papillaris parietalis sinister et dexter. In the right ventricle in man, there are two papillary parietal muscles: the mm. papillares anteriores et posteriores. On the septum of this ventricle there is, as in mammals, a muscle called the m. papillaris septalis medialis seu subarteriosus. Beside it are one or several smaller muscles, varying from one individual to another: the mm. papillares septales accessorii seu parvi. In the right ventricle of the mammalian heart is found, in addition to the m. parietalis septalis subarteriosus, already mentioned, a m. papillaris caudalis, more or less well developed in some species and, in the majority of mammals, the m. papillaris septalis cranials, which is always well developed. In certain mammals, there is, in rare cases, a m. papillaris septomarginalis seu parietalis. It may be said, in conclusion, that, in a large number of mammals, there is, on the internal surface of the external wall of the right ventricle, a reasonably well developed m. papillaris parietalis.", "PMID": 961336}
{"id": "pubmed23n0032_4325", "title": "Effects of sialoadenectomy and parotin hormone on the development of rat sponge-induced granulation tissue.", "content": "A study was made concerning the effects of sialoadenectomy and parotin hormone administration upon sponge-induced granulation tissue of young adult rats. The effects of sialoadenectomy alone and sialoadenectomy plus a single subcutaneous injection of parotin hormone, in two different doses (0.15 and 0.30 mg), on the development of 15-day-old granulation tissue were observed. The action of parotin hormone administration without sialoadenectomy was also investigated. The results have shown that sialoadenectomy produces a retardation effect upon the development of the tissue. This effect was partially inhibited by 0.15 mg and totally eliminated by the administration of 0.30 mg of the hormone. When administered in rats which were not sialoadenectomized, the dose of 0.30 mg caused increased fibroplasia and vascularization of granulation tissue, while a dose of 0.15 mg did not cause any significant difference when compared to the control tissue.", "contents": "Effects of sialoadenectomy and parotin hormone on the development of rat sponge-induced granulation tissue. A study was made concerning the effects of sialoadenectomy and parotin hormone administration upon sponge-induced granulation tissue of young adult rats. The effects of sialoadenectomy alone and sialoadenectomy plus a single subcutaneous injection of parotin hormone, in two different doses (0.15 and 0.30 mg), on the development of 15-day-old granulation tissue were observed. The action of parotin hormone administration without sialoadenectomy was also investigated. The results have shown that sialoadenectomy produces a retardation effect upon the development of the tissue. This effect was partially inhibited by 0.15 mg and totally eliminated by the administration of 0.30 mg of the hormone. When administered in rats which were not sialoadenectomized, the dose of 0.30 mg caused increased fibroplasia and vascularization of granulation tissue, while a dose of 0.15 mg did not cause any significant difference when compared to the control tissue.", "PMID": 961337}
{"id": "pubmed23n0032_4326", "title": "Secondary cartilages in growth and development of the symphysis menti in the hamster.", "content": "The early growth and development of the symphyseal joint and pertinent adjacent regions of the mandible in the hamster, Mesocricetus auratus, are described. The joint is patent throughout life and in adults contains a large fibrocartilage which mediates mandibular movements. Evidence is presented to show that this cartilage is formed by the fusion of Meckel's cartilage with two secondary cartilages that appear within the joint space. Evidence is also presented which suggests that cells of the symphyseal cartilage have the capacity to produce bone.", "contents": "Secondary cartilages in growth and development of the symphysis menti in the hamster. The early growth and development of the symphyseal joint and pertinent adjacent regions of the mandible in the hamster, Mesocricetus auratus, are described. The joint is patent throughout life and in adults contains a large fibrocartilage which mediates mandibular movements. Evidence is presented to show that this cartilage is formed by the fusion of Meckel's cartilage with two secondary cartilages that appear within the joint space. Evidence is also presented which suggests that cells of the symphyseal cartilage have the capacity to produce bone.", "PMID": 961338}
{"id": "pubmed23n0032_4327", "title": "Histochemical studies on the liver of Heteropneustes fossilis treated with carbon tetrachloride.", "content": "The effect of carbon tetrachloride on the liver of Heteropneustes fossilis has been studied. It results in centrolobular necrosis and cirrhosis. The hepatocyte nuclei are enlarged and balloon-shaped cells are visible at a few places. There is an increase at this stage in the DNA content of the hepatocyte nucleus. The cytoplasm is heavily laden with glycogen. Fatty infiltration starts and the fat content in the cytoplasm of the liver cells is increased. After 5 h of injection, the nuclei undergo lysis followed by ruptures in the cell membrane. The DNA content is greatly reduced. There is a marked depletion in the glycogen content. The bile canaliculi are filled with the extruded cell contents. The fatty infiltration increases at this stage.", "contents": "Histochemical studies on the liver of Heteropneustes fossilis treated with carbon tetrachloride. The effect of carbon tetrachloride on the liver of Heteropneustes fossilis has been studied. It results in centrolobular necrosis and cirrhosis. The hepatocyte nuclei are enlarged and balloon-shaped cells are visible at a few places. There is an increase at this stage in the DNA content of the hepatocyte nucleus. The cytoplasm is heavily laden with glycogen. Fatty infiltration starts and the fat content in the cytoplasm of the liver cells is increased. After 5 h of injection, the nuclei undergo lysis followed by ruptures in the cell membrane. The DNA content is greatly reduced. There is a marked depletion in the glycogen content. The bile canaliculi are filled with the extruded cell contents. The fatty infiltration increases at this stage.", "PMID": 961339}
{"id": "pubmed23n0032_4328", "title": "Ultrastructural development of bone marrow adipose cell.", "content": "The postnatal development of bone marrow adipose cells was studied by sequential examination of marrow tissue obtained from the tibia and femur of 1- to 8-week-old rabbits. The bone marrow adipocyte begins to develop 2 weeks after birth. Its cellular origin differs from the \"fibroblast-like\" cell that is the progenitor of the extramedullary adipocyte. The marrow pre-adipocyte is associated with the sinus endothelium and adventitial cells, but is not associated with collagen and does not contain abundant organized rough endoplasmic reticulum. Unlike the extramedullary adipocyte, the course of differentiation of the medullary adipocyte is not characterized by the appearance of glycogen; furthermore, during the coalescence of lipid droplets to form larger fat globules, cytoplasmic organelles, such as mitochondria and ribosomes, are trapped within the lipid substance to form intralipid vacuoles containing degraded cytoplasmic organelles. The possible significance of these morphologic differences between the developing bone marrow adipocyte and that of its extramedullary counterpart is discussed.", "contents": "Ultrastructural development of bone marrow adipose cell. The postnatal development of bone marrow adipose cells was studied by sequential examination of marrow tissue obtained from the tibia and femur of 1- to 8-week-old rabbits. The bone marrow adipocyte begins to develop 2 weeks after birth. Its cellular origin differs from the \"fibroblast-like\" cell that is the progenitor of the extramedullary adipocyte. The marrow pre-adipocyte is associated with the sinus endothelium and adventitial cells, but is not associated with collagen and does not contain abundant organized rough endoplasmic reticulum. Unlike the extramedullary adipocyte, the course of differentiation of the medullary adipocyte is not characterized by the appearance of glycogen; furthermore, during the coalescence of lipid droplets to form larger fat globules, cytoplasmic organelles, such as mitochondria and ribosomes, are trapped within the lipid substance to form intralipid vacuoles containing degraded cytoplasmic organelles. The possible significance of these morphologic differences between the developing bone marrow adipocyte and that of its extramedullary counterpart is discussed.", "PMID": 961340}
{"id": "pubmed23n0032_4329", "title": "Ultrastructural study of nerve terminals in the submucous plexus and mucous membrane after extirpation of the myenteric plexus.", "content": "An attempt is made to separate in the submucous plexus and mucous membrane of the chronically isolated intestine of the cat the fibers originating from the myenteric plexus and those originating from the submucous plexus by secondary degeneration after the extirpation of the myenteric plexus. A considerable part of the nerve processes in the submucous plexus originate from the cells of the myenteric ganglia and establish direct synaptic relationships between the ganglion cells, or are very close to the blood vessels. The numerous intact synapses between the different nerve elements in the submucous plexus point to local reflex connexions within the plexus itself. The degeneration of sub-epithelial nerve elements, containing always clear and dense-core (1,000-1,200 A in diameter) vesicles besides the numerous intact nerve elements, might indicate the presence of real sensory nerve processes in this layer. The terminal fibers remaining intact after removal of the myenteric plexus contain numerous medium-sized dense-core vesicles in potassium permanganate-fixed material. This suggests that there are cells containing 5-hydroxytryptamine in the submucous plexus.", "contents": "Ultrastructural study of nerve terminals in the submucous plexus and mucous membrane after extirpation of the myenteric plexus. An attempt is made to separate in the submucous plexus and mucous membrane of the chronically isolated intestine of the cat the fibers originating from the myenteric plexus and those originating from the submucous plexus by secondary degeneration after the extirpation of the myenteric plexus. A considerable part of the nerve processes in the submucous plexus originate from the cells of the myenteric ganglia and establish direct synaptic relationships between the ganglion cells, or are very close to the blood vessels. The numerous intact synapses between the different nerve elements in the submucous plexus point to local reflex connexions within the plexus itself. The degeneration of sub-epithelial nerve elements, containing always clear and dense-core (1,000-1,200 A in diameter) vesicles besides the numerous intact nerve elements, might indicate the presence of real sensory nerve processes in this layer. The terminal fibers remaining intact after removal of the myenteric plexus contain numerous medium-sized dense-core vesicles in potassium permanganate-fixed material. This suggests that there are cells containing 5-hydroxytryptamine in the submucous plexus.", "PMID": 961341}
{"id": "pubmed23n0032_4330", "title": "Nuclei and tracts of the di-mesencephalon of the parakeet.", "content": "A number of nuclei and fibre systems of the parakeet di-mesencephalon, as visible in transverse series stained with the methods of HAGGQVIST and of KLUVER and BARRERA, have been described. In the preparations used, in which both neurones and myelinated fibres are visible, a relatively great number of elements can be distinguished, because of differences in cell and fibre content. Probably these elements are more sharply circumscribed than in the pigeon and therefore more readily visible. A few of them have been described for the first time, but they require experimental substantiation. Typical features of some nuclei and fibre systems of the parakeet have been pointed out. Attributing a certain type to the di-mesencephalon of the parakeet proved to be unfeasible, because of a deficient knowledge both about the elements studied and to their aspect in a sufficient number of other avian species. The pictures produced may be helpful in recognizing nuclei and fibre systems in other birds in which they are less distinct.", "contents": "Nuclei and tracts of the di-mesencephalon of the parakeet. A number of nuclei and fibre systems of the parakeet di-mesencephalon, as visible in transverse series stained with the methods of HAGGQVIST and of KLUVER and BARRERA, have been described. In the preparations used, in which both neurones and myelinated fibres are visible, a relatively great number of elements can be distinguished, because of differences in cell and fibre content. Probably these elements are more sharply circumscribed than in the pigeon and therefore more readily visible. A few of them have been described for the first time, but they require experimental substantiation. Typical features of some nuclei and fibre systems of the parakeet have been pointed out. Attributing a certain type to the di-mesencephalon of the parakeet proved to be unfeasible, because of a deficient knowledge both about the elements studied and to their aspect in a sufficient number of other avian species. The pictures produced may be helpful in recognizing nuclei and fibre systems in other birds in which they are less distinct.", "PMID": 961342}
{"id": "pubmed23n0032_4331", "title": "Constitution and body proportions in different strains of rats. A study of race formation in breeding isolates.", "content": "(1) There are considerable differences in body constitution in different strains of rats, ranging from very robust to very gracile forms. The differences are greater in the larger males than in the smaller females. (2)Craniofacial and postcranial proportions of the trunk in domesticated strains of rats differ either uniformly or mosaically from the wild form. (3)Nasal shape differs greatly in rats ranging from extreme leptorrhine to extreme platyrrhine forms, giving a total range greater than in human populations. (4)Fisher 344 rats have long tails and extremities and Buffalo and GRL short tails and extremities. (5)Wistar and ACI rats have longer tails than wild rats but do not differ significantly from wild rats in the relative length of their other extremities. (6)The relationship between tail and extremity length is under genetic control which is concordant in Fisher, Buffalo and GRL rats, but discordant in the Lewis, Wistar and ACI strains. (7)There is no connection between relative extremity length and total body constitution since short-limbed strains occupy both the highest (Buffalo) and lowest (GRL) levels o f robusticity, and strains that do not differ in relative extremity length from wild rats differ greatly from each other in constitutional type (Wistar and ACI). (8)Differences of relative extremity length and nasal shape in rats have their parallels in human populations. But in human populations they follow Allen's ecological \"rule\" and can be duplicated experimentally. In rats here used, however, they do not result from any known ecological pressures, but from the genetic factors acting in breeding isolates.", "contents": "Constitution and body proportions in different strains of rats. A study of race formation in breeding isolates. (1) There are considerable differences in body constitution in different strains of rats, ranging from very robust to very gracile forms. The differences are greater in the larger males than in the smaller females. (2)Craniofacial and postcranial proportions of the trunk in domesticated strains of rats differ either uniformly or mosaically from the wild form. (3)Nasal shape differs greatly in rats ranging from extreme leptorrhine to extreme platyrrhine forms, giving a total range greater than in human populations. (4)Fisher 344 rats have long tails and extremities and Buffalo and GRL short tails and extremities. (5)Wistar and ACI rats have longer tails than wild rats but do not differ significantly from wild rats in the relative length of their other extremities. (6)The relationship between tail and extremity length is under genetic control which is concordant in Fisher, Buffalo and GRL rats, but discordant in the Lewis, Wistar and ACI strains. (7)There is no connection between relative extremity length and total body constitution since short-limbed strains occupy both the highest (Buffalo) and lowest (GRL) levels o f robusticity, and strains that do not differ in relative extremity length from wild rats differ greatly from each other in constitutional type (Wistar and ACI). (8)Differences of relative extremity length and nasal shape in rats have their parallels in human populations. But in human populations they follow Allen's ecological \"rule\" and can be duplicated experimentally. In rats here used, however, they do not result from any known ecological pressures, but from the genetic factors acting in breeding isolates.", "PMID": 961343}
{"id": "pubmed23n0032_4332", "title": "[Differention of the neural tube. Experimental study in chick and quail embryos].", "content": "In order to study the possible influence of adjacent structures upon differentiation and proliferation of the chick spinal cord, a piece of neural tube of chick embryos incubated for 2 days (stages 12-14 of HAMBURGER and HAMILTON) was extirpated and replaced by an equal graft from a donor embryo of the same age. The grafts were rotated 90 or 180 degrees around their longitudinal axis. The examination of the serial histological sections and the analysis of the distribution of mitotic density in the rotated spinal cord of embryos fixed 2-8 days after the operation led to the following results: the differences in morphogenesis and histogenesis between dorsal and ventral sectors of the spinal cord are already determined in 2-day-old chick embryos. The reversal of the neural tube at this time has no effect either upon the typical differentiation or the characteristic distribution of the mitotic cells in the spinal cord.", "contents": "[Differention of the neural tube. Experimental study in chick and quail embryos]. In order to study the possible influence of adjacent structures upon differentiation and proliferation of the chick spinal cord, a piece of neural tube of chick embryos incubated for 2 days (stages 12-14 of HAMBURGER and HAMILTON) was extirpated and replaced by an equal graft from a donor embryo of the same age. The grafts were rotated 90 or 180 degrees around their longitudinal axis. The examination of the serial histological sections and the analysis of the distribution of mitotic density in the rotated spinal cord of embryos fixed 2-8 days after the operation led to the following results: the differences in morphogenesis and histogenesis between dorsal and ventral sectors of the spinal cord are already determined in 2-day-old chick embryos. The reversal of the neural tube at this time has no effect either upon the typical differentiation or the characteristic distribution of the mitotic cells in the spinal cord.", "PMID": 961344}
{"id": "pubmed23n0032_4333", "title": "Localization of acid phosphatase activity in the medulla oblongata of the small brown dove, Streptopelia senegalensis, and white-throated munia, Uroloncha malabarica.", "content": "In the present study, acid phosphatase activity has been histochemically localized in the medulla oblongata (facialis part) of Streptopelia senegalensis and Uroloncha malabarica. It was noticed that the enzyme is present particularly in the motor nuclei and lysosomes of neuroglial cells of the medulla oblongata. However, enzymatic deposition appears to be somewhat more vigorous in S. senegalensis than in U. malabarica, thereby indicating their respective metabolic turnover and neuronal excitability. Simultaneously, the functional significance has been discussed in the light of differential localization.", "contents": "Localization of acid phosphatase activity in the medulla oblongata of the small brown dove, Streptopelia senegalensis, and white-throated munia, Uroloncha malabarica. In the present study, acid phosphatase activity has been histochemically localized in the medulla oblongata (facialis part) of Streptopelia senegalensis and Uroloncha malabarica. It was noticed that the enzyme is present particularly in the motor nuclei and lysosomes of neuroglial cells of the medulla oblongata. However, enzymatic deposition appears to be somewhat more vigorous in S. senegalensis than in U. malabarica, thereby indicating their respective metabolic turnover and neuronal excitability. Simultaneously, the functional significance has been discussed in the light of differential localization.", "PMID": 961345}
{"id": "pubmed23n0032_4334", "title": "Effect of corpora lutea grafted under renal capsule on pregnancy in the rabbit.", "content": "Implantation of the blastocyst and maintenance of pregnancy were examined by autografting corpora lutea under renal capsule in rabbits. Implantation of the blastocyst did not occur when the corpora lutea were autografted because of small amounts of lutein tissue, low secretory activity of lutein cells, and the period required for functional activity of grafts. Autografts of 4-day-old corpora lutea were capable of maintaining pregnancy after 15 days post coitum, when follicles and interstitial tissue remained intact. Pregnancy was maintained in a limited number of fetuses when 6-day-old corpora lutea were autografted in ovariectomized estradiol-treated does.", "contents": "Effect of corpora lutea grafted under renal capsule on pregnancy in the rabbit. Implantation of the blastocyst and maintenance of pregnancy were examined by autografting corpora lutea under renal capsule in rabbits. Implantation of the blastocyst did not occur when the corpora lutea were autografted because of small amounts of lutein tissue, low secretory activity of lutein cells, and the period required for functional activity of grafts. Autografts of 4-day-old corpora lutea were capable of maintaining pregnancy after 15 days post coitum, when follicles and interstitial tissue remained intact. Pregnancy was maintained in a limited number of fetuses when 6-day-old corpora lutea were autografted in ovariectomized estradiol-treated does.", "PMID": 961346}
{"id": "pubmed23n0032_4335", "title": "The ventromedial nucleus of the hypothalamus of sheep (Ovis aries) and the effects on food and water intake following its electrolytic destruction.", "content": "A description is presented of the ventromedial nucleus of the hypothalamus (VMN) of sheep following study of paraffin-embedded material stained with a Nissl and a myelin stain. The VMN is related to most structures in the ventrobasal hypothalamus and is ellipsoidally shaped with its poles tilted both medially and anteriorly. Three kinds of neurons can be distinguished under the high power of the light microscope, and other cellular variants are probably the three basic types seen in different orientations. The commonest neuron had bipolar processes and a basophilic line structure apparently curving over the surface of the nucleus. The food and water intake of four sheep with discrete destruction of the VMN is described and compared with sheep with anterior hypothalamic lesions and controls. Discrete destruction of the VMN was without effect on food and water intake. The conclusion is reached that more lateral and ventrolateral areas of the tuberal hypothalamus might be involved in satiety regulation in the sheep.", "contents": "The ventromedial nucleus of the hypothalamus of sheep (Ovis aries) and the effects on food and water intake following its electrolytic destruction. A description is presented of the ventromedial nucleus of the hypothalamus (VMN) of sheep following study of paraffin-embedded material stained with a Nissl and a myelin stain. The VMN is related to most structures in the ventrobasal hypothalamus and is ellipsoidally shaped with its poles tilted both medially and anteriorly. Three kinds of neurons can be distinguished under the high power of the light microscope, and other cellular variants are probably the three basic types seen in different orientations. The commonest neuron had bipolar processes and a basophilic line structure apparently curving over the surface of the nucleus. The food and water intake of four sheep with discrete destruction of the VMN is described and compared with sheep with anterior hypothalamic lesions and controls. Discrete destruction of the VMN was without effect on food and water intake. The conclusion is reached that more lateral and ventrolateral areas of the tuberal hypothalamus might be involved in satiety regulation in the sheep.", "PMID": 961347}
{"id": "pubmed23n0032_4336", "title": "[Comparative study of tracheal epithelium of different mammals].", "content": "Tracheal epithelia of ten different mammals were investigated with the light and the electron microscope. Characteristic differences were found concerning the thickness of the epithelia, the length of the cilia, the density of the cells in the epithelia, the numerical distribution of the different cells and their ultrastructure. Special attention was paid to the morphology of the kinetosomes. Brush cells and chromaffin cells, which are sparsely distributed in the different tracheal epithelia, were discussed.", "contents": "[Comparative study of tracheal epithelium of different mammals]. Tracheal epithelia of ten different mammals were investigated with the light and the electron microscope. Characteristic differences were found concerning the thickness of the epithelia, the length of the cilia, the density of the cells in the epithelia, the numerical distribution of the different cells and their ultrastructure. Special attention was paid to the morphology of the kinetosomes. Brush cells and chromaffin cells, which are sparsely distributed in the different tracheal epithelia, were discussed.", "PMID": 961348}
{"id": "pubmed23n0032_4337", "title": "[Esential comparative, morphological and topographical differences and characteristics of the cecum in man and domestic animals].", "content": "The caecum in man as a part of the large colon (intestinum crassum) differs morphologically and topographically from the caecum of domestic mammals. The caecum in domestic mammals differs not only from the human caecum but also comparatively morphologically and topographically among the domestic mammals. The appendix vermiformis does not exist in domestic mammals, which represents the main difference between the human caecum and that of domestic mammals. There are certainly some differences in the physiological function of the caecum in man and the caecum in domestic mammals, which are carnivores, omnivores, omnivores and herbivores.", "contents": "[Esential comparative, morphological and topographical differences and characteristics of the cecum in man and domestic animals]. The caecum in man as a part of the large colon (intestinum crassum) differs morphologically and topographically from the caecum of domestic mammals. The caecum in domestic mammals differs not only from the human caecum but also comparatively morphologically and topographically among the domestic mammals. The appendix vermiformis does not exist in domestic mammals, which represents the main difference between the human caecum and that of domestic mammals. There are certainly some differences in the physiological function of the caecum in man and the caecum in domestic mammals, which are carnivores, omnivores, omnivores and herbivores.", "PMID": 961349}
{"id": "pubmed23n0032_4338", "title": "Stature estimation from femur and humerus by regression and autometry.", "content": "Estimation of stature from a single extremity bone is a common forensic practice and many regression equations are given by various workers. In India, Pearson's formula is the most commonly used method to determine the height. These regression equations were subjected to verification on 194 (97 pairs) femora and 102 (51 pairs) humeri from India. It was seen that the Pearsonian formulae did not give exact results. The regression formulae differed statistically in both sexes in femur and humerus. These are calculated and given. This finding once again proves the necessity of having norms or formulae for the specific groups, when reliable results are required. In addition, the proportion between humerus length and femur length is also verified. This has evolutionary significance. In addition to the usual method as above, a method of the proportion these bones individually bear to the stature of the same person to which the bones belong is worked out both as a multiplication factor and percentage proportion to the body stature. It has been amply demonstrated and concluded that this method be called \"autometry\" and further that this seems to be more reliable method than the tedious yet variable and unreliable results the various formulae give. This autometry seems to have a consistency, being constant in both sexes and all races, thus evolving a \"human race autometry\".", "contents": "Stature estimation from femur and humerus by regression and autometry. Estimation of stature from a single extremity bone is a common forensic practice and many regression equations are given by various workers. In India, Pearson's formula is the most commonly used method to determine the height. These regression equations were subjected to verification on 194 (97 pairs) femora and 102 (51 pairs) humeri from India. It was seen that the Pearsonian formulae did not give exact results. The regression formulae differed statistically in both sexes in femur and humerus. These are calculated and given. This finding once again proves the necessity of having norms or formulae for the specific groups, when reliable results are required. In addition, the proportion between humerus length and femur length is also verified. This has evolutionary significance. In addition to the usual method as above, a method of the proportion these bones individually bear to the stature of the same person to which the bones belong is worked out both as a multiplication factor and percentage proportion to the body stature. It has been amply demonstrated and concluded that this method be called \"autometry\" and further that this seems to be more reliable method than the tedious yet variable and unreliable results the various formulae give. This autometry seems to have a consistency, being constant in both sexes and all races, thus evolving a \"human race autometry\".", "PMID": 961350}
{"id": "pubmed23n0032_4339", "title": "[The encephalus of the pond-loach, Misgurnus fossilis (L.), 1758 (fish, teleosts, Cobitidae). An example of sexual dimorphism].", "content": "The brain of the Pond-loach (Misgurnus fossilis) is very closely related to that of Cyprinidae. In the Medulla oblongata, the facial lobe-in relation with the skin taste buds, especially on the barbels-is bigger than the vagal lobes-which are related with the mouth taste buds. The intraspecific relations between brainweight and bodyweight or various body lengths show a real sexual dimorphism (brain dimegaly). The interspecific brain-bodyweight relationship shows an allometric coefficient of 0.505 in males and 0.485 in females, very close to that of the Rainbow trout. The encephalization index (63) is very close to that of the Tench, and a little lower than that of the Cyprinidae as a whole.", "contents": "[The encephalus of the pond-loach, Misgurnus fossilis (L.), 1758 (fish, teleosts, Cobitidae). An example of sexual dimorphism]. The brain of the Pond-loach (Misgurnus fossilis) is very closely related to that of Cyprinidae. In the Medulla oblongata, the facial lobe-in relation with the skin taste buds, especially on the barbels-is bigger than the vagal lobes-which are related with the mouth taste buds. The intraspecific relations between brainweight and bodyweight or various body lengths show a real sexual dimorphism (brain dimegaly). The interspecific brain-bodyweight relationship shows an allometric coefficient of 0.505 in males and 0.485 in females, very close to that of the Rainbow trout. The encephalization index (63) is very close to that of the Tench, and a little lower than that of the Cyprinidae as a whole.", "PMID": 961351}
{"id": "pubmed23n0032_4340", "title": "Karyometric studies of the nucleus preopticus in fish (Clarias batrachus) and toad (Bufo andersonii).", "content": "With the object of investigating the so called 'feedback' mechanism, whereby hormones from the various endocrine glands influence the hypothalamus in its regulation of the hypophysial tropic hormones, the activity of the nucleus preopticus (NPO) has been studied by means of karyometry under experimental conditions to understand the endocrine nature of the hypothalamic nuclei. (1) Oestrogen and testosterone propionate caused an increase in nuclear volume of the NPO cells, which is related to the synthesis of secretion. An increase of the nuclear volume is attributed to an increase of secretion. (2) Methylthiouracil (MTU) and thiourea bring about shrinkage in nuclear dimensions (p less than 0.001). Depletion of NSM is noticed in the hypothalamo-hypophysial neurosecretory system after MTU administration. (3) Two to five days' administration brings about depletion of NSM in the cells of the NPO. Eight to 15 days' metopiron treatment enhanced the activity of NPO cells. (4) Hypophysectomy caused shrinkage of the NPO cell nuclei, depletion of AF-stainable material in the cells of the NPO. ACTH treatment reversed these changes and brought about hypertrophy of the neurosecretory cells. (5) Neomercazole treatment and radiothyroidectomy bring about shrinkage in the NPO cell nuclei. Thyroidectomized toads when injected with thyroxine showed an enlargement of their nuclei.", "contents": "Karyometric studies of the nucleus preopticus in fish (Clarias batrachus) and toad (Bufo andersonii). With the object of investigating the so called 'feedback' mechanism, whereby hormones from the various endocrine glands influence the hypothalamus in its regulation of the hypophysial tropic hormones, the activity of the nucleus preopticus (NPO) has been studied by means of karyometry under experimental conditions to understand the endocrine nature of the hypothalamic nuclei. (1) Oestrogen and testosterone propionate caused an increase in nuclear volume of the NPO cells, which is related to the synthesis of secretion. An increase of the nuclear volume is attributed to an increase of secretion. (2) Methylthiouracil (MTU) and thiourea bring about shrinkage in nuclear dimensions (p less than 0.001). Depletion of NSM is noticed in the hypothalamo-hypophysial neurosecretory system after MTU administration. (3) Two to five days' administration brings about depletion of NSM in the cells of the NPO. Eight to 15 days' metopiron treatment enhanced the activity of NPO cells. (4) Hypophysectomy caused shrinkage of the NPO cell nuclei, depletion of AF-stainable material in the cells of the NPO. ACTH treatment reversed these changes and brought about hypertrophy of the neurosecretory cells. (5) Neomercazole treatment and radiothyroidectomy bring about shrinkage in the NPO cell nuclei. Thyroidectomized toads when injected with thyroxine showed an enlargement of their nuclei.", "PMID": 961352}
{"id": "pubmed23n0032_4341", "title": "Histochemical studies of the development of alkaline and acid phosphatase activities in the ovary and oviduct of the fowl (Gallus domesticus).", "content": "Alkaline and acid phosphatase activities were studied in the ovary and oviducts of pullets from the age of 2-32 weeks. Adult fowls were similarly studied. Alkaline phosphatase activity was present only in the glandular grooves and crypts of the immature oviducts. Alkaline phosphatase activity appeared at the pits of epithelial evaginations as glandular formation commenced. The young, non-secreting glands also showed the enzyme activity. But in the mature oviduct, alkaline phosphatase activity was confined to only the uterovaginal glands or sperm host glands and the epithelium of the vagina. In the ovary, alkaline phosphatase activity in the theca interna increased as the diameter of the follicles increased. Acid phosphatase activity was not present in the ovary, but in the oviduct, the enzyme activity was present in the uterine (shell gland) glands and in the uterovaginal epithelium and glands (sperm host glands). Alkaline phosphatase activity in the ovarian follicles and in the immature oviduct is thought to be related to histodifferentiation of these structures.", "contents": "Histochemical studies of the development of alkaline and acid phosphatase activities in the ovary and oviduct of the fowl (Gallus domesticus). Alkaline and acid phosphatase activities were studied in the ovary and oviducts of pullets from the age of 2-32 weeks. Adult fowls were similarly studied. Alkaline phosphatase activity was present only in the glandular grooves and crypts of the immature oviducts. Alkaline phosphatase activity appeared at the pits of epithelial evaginations as glandular formation commenced. The young, non-secreting glands also showed the enzyme activity. But in the mature oviduct, alkaline phosphatase activity was confined to only the uterovaginal glands or sperm host glands and the epithelium of the vagina. In the ovary, alkaline phosphatase activity in the theca interna increased as the diameter of the follicles increased. Acid phosphatase activity was not present in the ovary, but in the oviduct, the enzyme activity was present in the uterine (shell gland) glands and in the uterovaginal epithelium and glands (sperm host glands). Alkaline phosphatase activity in the ovarian follicles and in the immature oviduct is thought to be related to histodifferentiation of these structures.", "PMID": 961353}
{"id": "pubmed23n0032_4342", "title": "Histological and ultrastructural observations on the development of the lung of the fetal pig.", "content": "Lungs of fetal pigs having gestational ages ranging from 80 to 115 days were examined histologically and by electron microscopy. At 80 days bronchial epithelium was ciliated but bronchiolar cells were not and bronchial mucosal glands were absent. Peripheral regions consisted predominantly of mesenchymal tissue with glandular alveoli. 92 days marked the transition from the immature to the more mature lung type. Bronchial glands appeared and began to grow from the epithelium into the lamina propria, bronchiolar epithelial cells acquired cilia, and alveoli were becoming irregular in shape and had thinner interalveolar septa. Close contact between capillaries and alveolar epithelium established the blood-air barrier at many points. Differentiation of alveolar epithelium into types I and II pneumonocytes occurred at this stage and lamellated osmiophilic inclusion bodies were present in type II cells for the first time. The number of lamellated bodies increased progressively to term at 115 days.", "contents": "Histological and ultrastructural observations on the development of the lung of the fetal pig. Lungs of fetal pigs having gestational ages ranging from 80 to 115 days were examined histologically and by electron microscopy. At 80 days bronchial epithelium was ciliated but bronchiolar cells were not and bronchial mucosal glands were absent. Peripheral regions consisted predominantly of mesenchymal tissue with glandular alveoli. 92 days marked the transition from the immature to the more mature lung type. Bronchial glands appeared and began to grow from the epithelium into the lamina propria, bronchiolar epithelial cells acquired cilia, and alveoli were becoming irregular in shape and had thinner interalveolar septa. Close contact between capillaries and alveolar epithelium established the blood-air barrier at many points. Differentiation of alveolar epithelium into types I and II pneumonocytes occurred at this stage and lamellated osmiophilic inclusion bodies were present in type II cells for the first time. The number of lamellated bodies increased progressively to term at 115 days.", "PMID": 961354}
{"id": "pubmed23n0032_4343", "title": "Erythrophagocytosis by cells of the trophoblastic epithelium in the sheep placenta in different stages of gestation.", "content": "Erythrophagocytosis by the trophoblastic epithelial cells of the sheep placenta was studied in different stages of gestation. Light-microscopic observations showed that the erythrophagocytosis became more and more conspicuous with increasing stages of gestation. Electron-microscopically, this process appeared to take place in the same way as described for red cell destruction elsewhere. Light-microscopically, a high acid phosphatase activity could be demonstrated in the cells which took part in the process of erythrophagocytosis. Degenerating epithelial cells were found, the number of which appeared to increase with the stage of gestation. Under the electron microscope, the cytoplasm of these degenerating cells appeared to contain a diversity of crystals, myelin-like figures and irregular coarse concretions.", "contents": "Erythrophagocytosis by cells of the trophoblastic epithelium in the sheep placenta in different stages of gestation. Erythrophagocytosis by the trophoblastic epithelial cells of the sheep placenta was studied in different stages of gestation. Light-microscopic observations showed that the erythrophagocytosis became more and more conspicuous with increasing stages of gestation. Electron-microscopically, this process appeared to take place in the same way as described for red cell destruction elsewhere. Light-microscopically, a high acid phosphatase activity could be demonstrated in the cells which took part in the process of erythrophagocytosis. Degenerating epithelial cells were found, the number of which appeared to increase with the stage of gestation. Under the electron microscope, the cytoplasm of these degenerating cells appeared to contain a diversity of crystals, myelin-like figures and irregular coarse concretions.", "PMID": 961355}
{"id": "pubmed23n0032_4344", "title": "A morphological study of the carotid body and the fibre content of carotid nerve in the buffalo.", "content": "In the buffalo the carotid body is located in the periarterial connective tissue of the occipital artery immediately cranial to the bifurcation of the common carotid artery. The carotid body is lobulated and does not appear to contain chromaffin tissue. Nerve terminals have been seen in close relation to the glomus cells. A quantitative study of the fibre content of the carotid nerve and the carotid body has also been made. The myelinated fibres in the carotid nerve range from 278 to 473, with a mean of 368 +/- 39 fibres. The histogram of myelinated fibres is distinctly unimodal. Of the myelinated fibre population, 55.4% of fibres have diameters of 2 mum, 34.1% of 4 mum, 9.5% of 6 mum and 1% have diameters of 8 mum. The mean greatest sagittal sectional area of the carotid body is 0.58 +/- 0.09 mm2. The mean density (per mm2) of glomus cells is 9,216 +/- 378. It appears that the organ is highly vascular (473 +/- 56 blood vessels/mm2 area). The mean size of the glomus cells is 7.85 +/- 0.46 mum X 10.02 +/- 0.64 mum (short diameter X long diameter), whereas the mean size of their nuclei is 4.96 +/- 0.19 mum X 5.52 +/- 0.27 mum.", "contents": "A morphological study of the carotid body and the fibre content of carotid nerve in the buffalo. In the buffalo the carotid body is located in the periarterial connective tissue of the occipital artery immediately cranial to the bifurcation of the common carotid artery. The carotid body is lobulated and does not appear to contain chromaffin tissue. Nerve terminals have been seen in close relation to the glomus cells. A quantitative study of the fibre content of the carotid nerve and the carotid body has also been made. The myelinated fibres in the carotid nerve range from 278 to 473, with a mean of 368 +/- 39 fibres. The histogram of myelinated fibres is distinctly unimodal. Of the myelinated fibre population, 55.4% of fibres have diameters of 2 mum, 34.1% of 4 mum, 9.5% of 6 mum and 1% have diameters of 8 mum. The mean greatest sagittal sectional area of the carotid body is 0.58 +/- 0.09 mm2. The mean density (per mm2) of glomus cells is 9,216 +/- 378. It appears that the organ is highly vascular (473 +/- 56 blood vessels/mm2 area). The mean size of the glomus cells is 7.85 +/- 0.46 mum X 10.02 +/- 0.64 mum (short diameter X long diameter), whereas the mean size of their nuclei is 4.96 +/- 0.19 mum X 5.52 +/- 0.27 mum.", "PMID": 961356}
{"id": "pubmed23n0032_4345", "title": "[Electron microscopic study of the development of the seminiferous tubule from birth to the end of the 1st year].", "content": "The development of the seminiferous tubule in the first year of life was studied with the electron microscope in five biopsies of normal testes. When HCG stimulation ceases, the fetal Sertoli cell changes into the Sa-Sertoli cell. The possibility of the fetal Sertoli cell having a hormone producing role is discussed. Gonocytes can be detected until the third month post natum. In addition to the gonocytes fetal, transitional, Ap and Ad spermatogonia are also present in the first year of life.", "contents": "[Electron microscopic study of the development of the seminiferous tubule from birth to the end of the 1st year]. The development of the seminiferous tubule in the first year of life was studied with the electron microscope in five biopsies of normal testes. When HCG stimulation ceases, the fetal Sertoli cell changes into the Sa-Sertoli cell. The possibility of the fetal Sertoli cell having a hormone producing role is discussed. Gonocytes can be detected until the third month post natum. In addition to the gonocytes fetal, transitional, Ap and Ad spermatogonia are also present in the first year of life.", "PMID": 961357}
{"id": "pubmed23n0032_4346", "title": "The structure of the suprarenal venous system and its possible functional significance.", "content": "The structure of the suprarenal venous system of 25 human suprarenal glands has been studied. From a structural point of view, four portions of the suprarenal vein have been distinguished: the proximal section, the hilus section, the section of the prehilus collaterals, and the distal section. In the hilus section and the section of the prehilus collaterals, the structure of the tunica media is more complex. In the hilus section, spiral fibres are found besides the longitudinal muscle fibres. Three strata of fibres can be observed in the section of the prehilus collaterals at the point where these veins drain into the central vein: two longitudinal strata, external and internal, and a third intermediate stratum corresponding to the looped fibres. The spiral and looped fibres, on contracting, can function as a sphincter. The longitudinal fibres, by means of the traction they exert on the stroma and connective capsule of the suprarenal, increase the intraglandular pressure while decreasing the periglandular pressure. A sympathetic stimulus provokes an increase in the secretion of adrenalin, and, at the same time, a contraction of the muscle fibres of the suprarenal venous system. Under these conditions, the venous return makes use of the emissary veins; and in this way, the secreted adrenalin establishes a close contact with the cortico-suprarenal parenchyma.", "contents": "The structure of the suprarenal venous system and its possible functional significance. The structure of the suprarenal venous system of 25 human suprarenal glands has been studied. From a structural point of view, four portions of the suprarenal vein have been distinguished: the proximal section, the hilus section, the section of the prehilus collaterals, and the distal section. In the hilus section and the section of the prehilus collaterals, the structure of the tunica media is more complex. In the hilus section, spiral fibres are found besides the longitudinal muscle fibres. Three strata of fibres can be observed in the section of the prehilus collaterals at the point where these veins drain into the central vein: two longitudinal strata, external and internal, and a third intermediate stratum corresponding to the looped fibres. The spiral and looped fibres, on contracting, can function as a sphincter. The longitudinal fibres, by means of the traction they exert on the stroma and connective capsule of the suprarenal, increase the intraglandular pressure while decreasing the periglandular pressure. A sympathetic stimulus provokes an increase in the secretion of adrenalin, and, at the same time, a contraction of the muscle fibres of the suprarenal venous system. Under these conditions, the venous return makes use of the emissary veins; and in this way, the secreted adrenalin establishes a close contact with the cortico-suprarenal parenchyma.", "PMID": 961358}
{"id": "pubmed23n0032_4347", "title": "Vascular patterns in the chick embryo spinal cord in normal and experimentally modified development.", "content": "The development of the vascular patterns of the lumbosacral segments of the chick embryo spinal cord has been analyzed in normal as well as in experimental conditions, after complete removal of the hind limb primordium. The results indicate that in both conditions the development of the vascular network follows the time-space sequence of the cytoarchitectural modifications of the cord, supporting the view that the vascularization does not exert a morphogenetic influence.", "contents": "Vascular patterns in the chick embryo spinal cord in normal and experimentally modified development. The development of the vascular patterns of the lumbosacral segments of the chick embryo spinal cord has been analyzed in normal as well as in experimental conditions, after complete removal of the hind limb primordium. The results indicate that in both conditions the development of the vascular network follows the time-space sequence of the cytoarchitectural modifications of the cord, supporting the view that the vascularization does not exert a morphogenetic influence.", "PMID": 961359}
{"id": "pubmed23n0032_4348", "title": "Ultrastructure of the neural canal closure in the chicken embryo.", "content": "We have studied the neuroectoderm of the chicken embryo, from the beginning of somitic segmentation up to the state at which it has seven somities, i.e. the period covering the passage from the 'neural canal' into the 'neural tube'. This paper was devoted mainly to the study of the ultrastructural cytodifferentiation which takes place during the stages in which the neural canal closes up, and at the level of the first area of contact between the 'neural crests'-roughly at the level of the third somite. We used eggs from a hen of the White Leghorn breed, incubated at 38 degrees C, from which we extracted chicken embryos after 24-30 h of incubation, corresponding to Hamburger-Hamilton's stages 7, 8 and 9. Thus we were able to obtain several series of embryos with three, six and seven somites. The neural canal, or tube, at the level of the third somite, was fixed in glutaraldehyde at 6.25% for 30 min and postfixed in 1% osmium tetroxide for 2 h embedded in Araldite, and the sections were then stained with lead citrate. We observed that the vacuoles in the free edge of the neural canal gradually disappear as the canal closes up, while we gradually witness the appearance of the 'closure apparatus' (or the safety or occlusion apparatus) of what is beginning to form the ependymal epithelium, and the first rudimentary outlines of the cilia. All these changes begin to be observed at the seven-somite stage, i.e. when the neural canal is beginning to close up. The 'closure apparatus' consists of a number of intercellular joint complexes, of the 'close-join't type, between which we observe a number of fine filaments, like a terminal velum', or veil, which we call 'interconnecting filaments'. In the 'raphe', whereby contact is established between the neural crests, we observe the initial stages of fusion between the vacuolated edges, with the plasmatic membrane of these cells forming very fine cytoplasmic 'tongues' which interdigitate with cells from the opposite neural crest and finally constitute the so-called close joints.", "contents": "Ultrastructure of the neural canal closure in the chicken embryo. We have studied the neuroectoderm of the chicken embryo, from the beginning of somitic segmentation up to the state at which it has seven somities, i.e. the period covering the passage from the 'neural canal' into the 'neural tube'. This paper was devoted mainly to the study of the ultrastructural cytodifferentiation which takes place during the stages in which the neural canal closes up, and at the level of the first area of contact between the 'neural crests'-roughly at the level of the third somite. We used eggs from a hen of the White Leghorn breed, incubated at 38 degrees C, from which we extracted chicken embryos after 24-30 h of incubation, corresponding to Hamburger-Hamilton's stages 7, 8 and 9. Thus we were able to obtain several series of embryos with three, six and seven somites. The neural canal, or tube, at the level of the third somite, was fixed in glutaraldehyde at 6.25% for 30 min and postfixed in 1% osmium tetroxide for 2 h embedded in Araldite, and the sections were then stained with lead citrate. We observed that the vacuoles in the free edge of the neural canal gradually disappear as the canal closes up, while we gradually witness the appearance of the 'closure apparatus' (or the safety or occlusion apparatus) of what is beginning to form the ependymal epithelium, and the first rudimentary outlines of the cilia. All these changes begin to be observed at the seven-somite stage, i.e. when the neural canal is beginning to close up. The 'closure apparatus' consists of a number of intercellular joint complexes, of the 'close-join't type, between which we observe a number of fine filaments, like a terminal velum', or veil, which we call 'interconnecting filaments'. In the 'raphe', whereby contact is established between the neural crests, we observe the initial stages of fusion between the vacuolated edges, with the plasmatic membrane of these cells forming very fine cytoplasmic 'tongues' which interdigitate with cells from the opposite neural crest and finally constitute the so-called close joints.", "PMID": 961360}
{"id": "pubmed23n0032_4349", "title": "Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains.", "content": "Studies were carried out on the nuclear size of embryonic mouse brain monolayer cells at various cell densities. The variation in cell density resulted from different initial cell numbers. The nuclear area was measured area-analytically with the Quantimet, the determination of cell population was measured linear-analytically using the Digiscan. Nuclear size remains constant in a cell culture of 10(4) up to 8.3 X 10(4) cells ml, which corresponds to a cell density of about 25-160 cells/unit. A significant diminution in mean nuclear size of the cell nucleus occurs in the case of increased cell densities of 215 and 277 cells/unit, corresponding to a cell culture of 2.5 X 10(5) AND 7.3 X 10(5) cells ml. This diminution may be connected with the accumulation of G1 cell nuclei in cultures with contact-dependent growth inhibition.", "contents": "Influence of cell density of nuclear size in monolayer cells from embryonic mouse brains. Studies were carried out on the nuclear size of embryonic mouse brain monolayer cells at various cell densities. The variation in cell density resulted from different initial cell numbers. The nuclear area was measured area-analytically with the Quantimet, the determination of cell population was measured linear-analytically using the Digiscan. Nuclear size remains constant in a cell culture of 10(4) up to 8.3 X 10(4) cells ml, which corresponds to a cell density of about 25-160 cells/unit. A significant diminution in mean nuclear size of the cell nucleus occurs in the case of increased cell densities of 215 and 277 cells/unit, corresponding to a cell culture of 2.5 X 10(5) AND 7.3 X 10(5) cells ml. This diminution may be connected with the accumulation of G1 cell nuclei in cultures with contact-dependent growth inhibition.", "PMID": 961361}
{"id": "pubmed23n0032_4350", "title": "Correlation between the structure of the wall of the bone lacuna and the localization of the osteocyte within this lacuna. Electron-microscopic studies.", "content": "A correlation between the structure of the wall of the bone lacuna and the localization of the osteocyte within this lacuna was the object of investigation under light and electron microscopes. On the basis of the structural divergences detected, the author distinguished zones A and B in the osteocyte situated in the lacuna. Zone B is characterized by a parallel, compact system of collagen fibrils which form a characteristic ridge in the lacunar wall, a continuous demarcating line bordering this wall, the osteocyte cytoplasm in this part lying far from the lacuna wall and surrounding the nucleus with a narrow band, and the pericellular space which is wide here and containing a large number of mucopolysaccharides. This picture may correspond to the area of temporary non-reactivity of the osteocyte. Zone A is characterized by a loosened and disordered system of collagen fibrils forming the lacunar wall. These fibrils being exposed in this place by nature, the demarcating line bordering the lacunar wall is broken in some places, the osteocyte cytoplasm is considerably concentrated around the nucleus and contains a large number of organelles. It also lies closer to the lacunar wall, which results in a decreased width of the pericellular space and a diminished mucopolysaccharide content in this part of the lacuna. Structural variations in the lacuna wall and its space (zones A and B) observed in the images obtained with a normal bone in the light and electron microscopes show their immediate dependence on the position of the osteocyte in the lacuna, which may be associated with the osteocyte activity. On the other hand, the occurrence of significant changes in a definite zone (zone A) of the lacuna only points to the oriented character of this activity.", "contents": "Correlation between the structure of the wall of the bone lacuna and the localization of the osteocyte within this lacuna. Electron-microscopic studies. A correlation between the structure of the wall of the bone lacuna and the localization of the osteocyte within this lacuna was the object of investigation under light and electron microscopes. On the basis of the structural divergences detected, the author distinguished zones A and B in the osteocyte situated in the lacuna. Zone B is characterized by a parallel, compact system of collagen fibrils which form a characteristic ridge in the lacunar wall, a continuous demarcating line bordering this wall, the osteocyte cytoplasm in this part lying far from the lacuna wall and surrounding the nucleus with a narrow band, and the pericellular space which is wide here and containing a large number of mucopolysaccharides. This picture may correspond to the area of temporary non-reactivity of the osteocyte. Zone A is characterized by a loosened and disordered system of collagen fibrils forming the lacunar wall. These fibrils being exposed in this place by nature, the demarcating line bordering the lacunar wall is broken in some places, the osteocyte cytoplasm is considerably concentrated around the nucleus and contains a large number of organelles. It also lies closer to the lacunar wall, which results in a decreased width of the pericellular space and a diminished mucopolysaccharide content in this part of the lacuna. Structural variations in the lacuna wall and its space (zones A and B) observed in the images obtained with a normal bone in the light and electron microscopes show their immediate dependence on the position of the osteocyte in the lacuna, which may be associated with the osteocyte activity. On the other hand, the occurrence of significant changes in a definite zone (zone A) of the lacuna only points to the oriented character of this activity.", "PMID": 961362}
{"id": "pubmed23n0032_4351", "title": "Variations in kidney ultrastructure with a variety of paraformaldehyde fixation techniques.", "content": "Variations in fine structure of glomeruli and proximal tubules were studied to determine the results of several fixation techniques with paraformaldehyde. Immersion fixation techniques generally resulted in poor fixation, while the quality of fixation by perfusion was directly related to the concentration of the paraformaldehyde. The best technique was fixation by perfusion and immersion in 3% paraformaldehyde for 2 h followed by a post-fixation in 1% OsO4 for 1 h. Intracellular edema was noted in all epithelial, mesangial and proximal tubular cells that were from poorly fixed kidneys. Swollen mitochondria and the dilated endoplasmic reticulum were common in many of those cells. Mesangial and proximal tubular cells were the first to swell. Poor fixation produced the early stages of autolysis. The criteria for good paraformaldehyde fixation are discussed.", "contents": "Variations in kidney ultrastructure with a variety of paraformaldehyde fixation techniques. Variations in fine structure of glomeruli and proximal tubules were studied to determine the results of several fixation techniques with paraformaldehyde. Immersion fixation techniques generally resulted in poor fixation, while the quality of fixation by perfusion was directly related to the concentration of the paraformaldehyde. The best technique was fixation by perfusion and immersion in 3% paraformaldehyde for 2 h followed by a post-fixation in 1% OsO4 for 1 h. Intracellular edema was noted in all epithelial, mesangial and proximal tubular cells that were from poorly fixed kidneys. Swollen mitochondria and the dilated endoplasmic reticulum were common in many of those cells. Mesangial and proximal tubular cells were the first to swell. Poor fixation produced the early stages of autolysis. The criteria for good paraformaldehyde fixation are discussed.", "PMID": 961363}
{"id": "pubmed23n0032_4352", "title": "Effect of whole-body irradiation on the synethesis of ribonucleic acid associated with nuclear ribonucleoprotein particles of rat liver.", "content": "Synthesis of the RNA of rat liver nuclear RNP particles (\"informofers\") was studied within 12 hrs after 1930 rad whole-body gamma-irradiation. 14C-orotic acid was administratered intravenously and nuclear RNP particles were extracted by 0.1 M and by 0.3 M salt solutions at pH 8.0. Radioactivity of the RNP particles 1.5--2.0 times higher than that of the unirradiated controls up to 6 hrs after irradiation, exhibiting a maximum at the first hour. The labelling of the 0.3 M RNP particles was higher and chased less rapidly than that of the 0.1 M RNP particles. The RNA to protein ratio and the protein composition of the RNP the RNP particles did not change after irradiation as examined by CsCl density gradient centrifugation and by acrylamide gel electrophoresis, respectively. In the cytoplasm enhanced synthesis of rapidly labelled RNA sedimenting with 40 S cytoplasmic RNP paricles could also be demonstrated after irradiation. It is concluded that the synthesis of messenger-type RNA associated with liver nuclear RNP particles increases and more RNA is transferred from the nucleus to the cytoplasm after whole-body irradiation.", "contents": "Effect of whole-body irradiation on the synethesis of ribonucleic acid associated with nuclear ribonucleoprotein particles of rat liver. Synthesis of the RNA of rat liver nuclear RNP particles (\"informofers\") was studied within 12 hrs after 1930 rad whole-body gamma-irradiation. 14C-orotic acid was administratered intravenously and nuclear RNP particles were extracted by 0.1 M and by 0.3 M salt solutions at pH 8.0. Radioactivity of the RNP particles 1.5--2.0 times higher than that of the unirradiated controls up to 6 hrs after irradiation, exhibiting a maximum at the first hour. The labelling of the 0.3 M RNP particles was higher and chased less rapidly than that of the 0.1 M RNP particles. The RNA to protein ratio and the protein composition of the RNP the RNP particles did not change after irradiation as examined by CsCl density gradient centrifugation and by acrylamide gel electrophoresis, respectively. In the cytoplasm enhanced synthesis of rapidly labelled RNA sedimenting with 40 S cytoplasmic RNP paricles could also be demonstrated after irradiation. It is concluded that the synthesis of messenger-type RNA associated with liver nuclear RNP particles increases and more RNA is transferred from the nucleus to the cytoplasm after whole-body irradiation.", "PMID": 961365}
{"id": "pubmed23n0032_4353", "title": "Binding of alkylamines to trypsin as measured by difference spectroscopy.", "content": "The effect of n-propylamine, n-butylamine and n-pentylamine on trypsin at pH 5.4, at 25 degrees and 7 degrees C was investigated by the difference spectroscopic technique. The difference spectra were found to be similar for all three alkylamines tested. The difference between the absorbance minimum near 290 nm and the absorbance maximum near 297 nm taken as the measure of perturbation, the following values were obtained for the dissociation constant of the enzyme-ligand complex as calculated from the dependence of this perturbation upon alkylamine concentration at 25 degrees: propylamine, 6.0 X 10(-3) M; butylamine, 3.0 X 10(-3) M; pentylamine, 4.3 X 10(-4) M; and at 7 degrees: butylamine: 5.6 X 10(-3) M the chromophores situated in the active centre. It seems probable that the binding of an alkylamine gives rise to a conformational change which results in the rotation of Tyr-228 and Trp-215 residues towards the hydrophobic surroundings. This dislocation is a part of the minute change that leads to the activation of the catalytic site.", "contents": "Binding of alkylamines to trypsin as measured by difference spectroscopy. The effect of n-propylamine, n-butylamine and n-pentylamine on trypsin at pH 5.4, at 25 degrees and 7 degrees C was investigated by the difference spectroscopic technique. The difference spectra were found to be similar for all three alkylamines tested. The difference between the absorbance minimum near 290 nm and the absorbance maximum near 297 nm taken as the measure of perturbation, the following values were obtained for the dissociation constant of the enzyme-ligand complex as calculated from the dependence of this perturbation upon alkylamine concentration at 25 degrees: propylamine, 6.0 X 10(-3) M; butylamine, 3.0 X 10(-3) M; pentylamine, 4.3 X 10(-4) M; and at 7 degrees: butylamine: 5.6 X 10(-3) M the chromophores situated in the active centre. It seems probable that the binding of an alkylamine gives rise to a conformational change which results in the rotation of Tyr-228 and Trp-215 residues towards the hydrophobic surroundings. This dislocation is a part of the minute change that leads to the activation of the catalytic site.", "PMID": 961366}
{"id": "pubmed23n0032_4354", "title": "Investigation of hydration of macromolecules. II. Studies on poly-(vinyl-alcohol) solutions by microwave dielectric method.", "content": "Microwave conductivity and dielectric condstant of poly-(vinyl-alcohol) (PVA) solutions of 0--10 per cent were determined at 2--4 GHz frequency in the temperature range from 25 degrees C to 35 degrees C. The low-frequency conductivities of the solutions were measured at 20 kHz in the same concentration and temperature range. Microwave conductivity was proportional to the square of frequency, and the axial section extrapolated to zero frequency was higher than low-frequency conductivity. This difference in conductivity can probably be attributed to the relaxation processes of water molecules interacting directly with macromolecules. The relaxation wavelength calculated from the frequency dependence of microwave conductivity increased and the dielectric constant of the solutions decreased with increasing macromolecular concentration in a linear way. The activation energy calculated from its temperature dependence was 4.0 +/- 1.0 kcal/mol. The products of self-diffusion coefficient and macroviscosity were compared with that of self-diffusion coefficient and microviscosity calculated from relaxation wavelength. From this the conclusion can be drawn that the self-diffusion of water is influenced by the concentration dependent microviscosity. The hydration numbers calculated by different methods give divergen results the discussion of which requires further investigations.", "contents": "Investigation of hydration of macromolecules. II. Studies on poly-(vinyl-alcohol) solutions by microwave dielectric method. Microwave conductivity and dielectric condstant of poly-(vinyl-alcohol) (PVA) solutions of 0--10 per cent were determined at 2--4 GHz frequency in the temperature range from 25 degrees C to 35 degrees C. The low-frequency conductivities of the solutions were measured at 20 kHz in the same concentration and temperature range. Microwave conductivity was proportional to the square of frequency, and the axial section extrapolated to zero frequency was higher than low-frequency conductivity. This difference in conductivity can probably be attributed to the relaxation processes of water molecules interacting directly with macromolecules. The relaxation wavelength calculated from the frequency dependence of microwave conductivity increased and the dielectric constant of the solutions decreased with increasing macromolecular concentration in a linear way. The activation energy calculated from its temperature dependence was 4.0 +/- 1.0 kcal/mol. The products of self-diffusion coefficient and macroviscosity were compared with that of self-diffusion coefficient and microviscosity calculated from relaxation wavelength. From this the conclusion can be drawn that the self-diffusion of water is influenced by the concentration dependent microviscosity. The hydration numbers calculated by different methods give divergen results the discussion of which requires further investigations.", "PMID": 961367}
{"id": "pubmed23n0032_4355", "title": "Comparison of the redox states of different tissues and the types of acetylcholine effect.", "content": "By simultaneous recording of myograms, cardiomyograms and tissue redox state potentials (RSP) it was established that in tissues with higher RSP levels (+170 mV or more; rectus muscle, smooth muscle of the stomach) acetylcholine (Ach) exerted a depolarizing type of action, while in the frog heart ventricle (with RSP values lower than +170 mV) the mediator triggered a hyperpolarizing type Ach effect. Since following an appropriate adjustment of RSP values by exogenous redox agents the depolarizing and/or hyperpolarizing Ach effects can be modelled on any organs, independently of the original type of effect of Ach, it may be assumed that the existing redox state plays an important role in the determination of the depolarizing and/or hyperpolarizing effect of Ach.", "contents": "Comparison of the redox states of different tissues and the types of acetylcholine effect. By simultaneous recording of myograms, cardiomyograms and tissue redox state potentials (RSP) it was established that in tissues with higher RSP levels (+170 mV or more; rectus muscle, smooth muscle of the stomach) acetylcholine (Ach) exerted a depolarizing type of action, while in the frog heart ventricle (with RSP values lower than +170 mV) the mediator triggered a hyperpolarizing type Ach effect. Since following an appropriate adjustment of RSP values by exogenous redox agents the depolarizing and/or hyperpolarizing Ach effects can be modelled on any organs, independently of the original type of effect of Ach, it may be assumed that the existing redox state plays an important role in the determination of the depolarizing and/or hyperpolarizing effect of Ach.", "PMID": 961368}
{"id": "pubmed23n0032_4356", "title": "[Can EEG contribute to the diagnosis of bilateral thrombosis of the carotid artery? (author's transl)].", "content": "The EEG of 48 patients suffering from either an unilateral or a bilateral thrombosis of the carotid artery are compared, and the most discriminative data of the recordings in these two conditions are stressed.", "contents": "[Can EEG contribute to the diagnosis of bilateral thrombosis of the carotid artery? (author's transl)]. The EEG of 48 patients suffering from either an unilateral or a bilateral thrombosis of the carotid artery are compared, and the most discriminative data of the recordings in these two conditions are stressed.", "PMID": 961370}
{"id": "pubmed23n0032_4357", "title": "The syndrome of the posterior retroparotid space.", "content": "A case of Villaret's syndrome, possibly secondary to herpes zoster, is presented. The topography of the lesion in this rare disease is discussed as well as the differential diagnosis.", "contents": "The syndrome of the posterior retroparotid space. A case of Villaret's syndrome, possibly secondary to herpes zoster, is presented. The topography of the lesion in this rare disease is discussed as well as the differential diagnosis.", "PMID": 961371}
{"id": "pubmed23n0032_4358", "title": "Regressive language in severe head injury.", "content": "In a follow-up study of 50 patients with severe head injuries three patients had echolalia. One patient with initially global aphasia had echolalia for some weeks when he started talking. Another patient with severe diffuse brain damage, dementia, and emotional regression had echolalia. The dysfunction was considered a detour performance. In the third patient echolalia and palilalia were details in a total pattern of regression lasting for months. The patient, who had extensive frontal atrophy secondary to a very severe head trauma, presented an extreme state of regression returning to a foetal-body pattern and behaving like a baby.", "contents": "Regressive language in severe head injury. In a follow-up study of 50 patients with severe head injuries three patients had echolalia. One patient with initially global aphasia had echolalia for some weeks when he started talking. Another patient with severe diffuse brain damage, dementia, and emotional regression had echolalia. The dysfunction was considered a detour performance. In the third patient echolalia and palilalia were details in a total pattern of regression lasting for months. The patient, who had extensive frontal atrophy secondary to a very severe head trauma, presented an extreme state of regression returning to a foetal-body pattern and behaving like a baby.", "PMID": 961376}
{"id": "pubmed23n0032_4359", "title": "In vitro polarographic studies of rat brain oxygen uptake in simulated uraemia and hepatic coma.", "content": "A polarographic technique has been used to study the oxygen uptake of rat brain homogenates incubated with metabolites known to accumulate in uraemia and hepatic coma. In general, results with individual metabolites were in agreement with those previously obtained with brain slices using the Warburg technique. Inhibition was induced at higher concentrations with urea, creatine, indoxysulphate, guanidine, dimethylamine hydrochloride, ammonium chloride, taurocholate, glycodeoxycholate, sodium deoxycholate, skalote and indole. Serum and ultrafiltrate from drowsy uraemic patients also caused inhibition, and this was reversed by renal dialysis. Similarly serum and ultrafiltrate from patients in hepatic coma inhibited oxygen uptake but in this instance reversal of the inhibition was not demonstrated following exchange blood transfusion to the patients.", "contents": "In vitro polarographic studies of rat brain oxygen uptake in simulated uraemia and hepatic coma. A polarographic technique has been used to study the oxygen uptake of rat brain homogenates incubated with metabolites known to accumulate in uraemia and hepatic coma. In general, results with individual metabolites were in agreement with those previously obtained with brain slices using the Warburg technique. Inhibition was induced at higher concentrations with urea, creatine, indoxysulphate, guanidine, dimethylamine hydrochloride, ammonium chloride, taurocholate, glycodeoxycholate, sodium deoxycholate, skalote and indole. Serum and ultrafiltrate from drowsy uraemic patients also caused inhibition, and this was reversed by renal dialysis. Similarly serum and ultrafiltrate from patients in hepatic coma inhibited oxygen uptake but in this instance reversal of the inhibition was not demonstrated following exchange blood transfusion to the patients.", "PMID": 961377}
{"id": "pubmed23n0032_4360", "title": "[Pathological and biochemical studies on a neonatal case of argininosuccinic aciduria (author's transl)].", "content": "A newborn infant affected by argininosuccinic aciduria was treated with a hypoproteinaemic diet and survived up to the age of three months. Post mortem biochemical studies have confirmed the absence of argininosuccinase activity in kidney, liver and brain and a marked storage of argininosuccinic acid in the brain. The histological study of the central nervous system shows no Alzheimer type II cells; there is neither demyelination nor spongiosis. Such results are totally different from those recorded in untreated cases; they represent very likely the effects of the diet which has controlled the hyperammoniaemia and prevented a deficiency of arginine.", "contents": "[Pathological and biochemical studies on a neonatal case of argininosuccinic aciduria (author's transl)]. A newborn infant affected by argininosuccinic aciduria was treated with a hypoproteinaemic diet and survived up to the age of three months. Post mortem biochemical studies have confirmed the absence of argininosuccinase activity in kidney, liver and brain and a marked storage of argininosuccinic acid in the brain. The histological study of the central nervous system shows no Alzheimer type II cells; there is neither demyelination nor spongiosis. Such results are totally different from those recorded in untreated cases; they represent very likely the effects of the diet which has controlled the hyperammoniaemia and prevented a deficiency of arginine.", "PMID": 961372}
{"id": "pubmed23n0032_4361", "title": "F-wave velocity in motor neurone disease.", "content": "The F-wave velocity in the central segment (axilla to spinal cord) was studied employing the \"collison technique\" described by Kimura (1974), and compared with the conduction velocity obtained with the usual methods. In 25 normal subjects the F-wave velocity increased proceeding proximally, reaching the maximum values in the central tract (64.86 +/- 2.23 m/sec in ulnar nerve). In 11 patients affected by motor neurone disease and 11 patients affected by amyotrophic lateral sclerosis the F-wave velocity decreased significantly proceeding proximally and the minimum values were found in the central tract (52.51 +/- 2.15 m/sec in MND and 48.64 +/- 5.60 m/sec in ALS). We therefore suggest the use of F-wave velocity as a more complete element for precise localization of the lesion in the central segment when the motoneurone is primarily involved.", "contents": "F-wave velocity in motor neurone disease. The F-wave velocity in the central segment (axilla to spinal cord) was studied employing the \"collison technique\" described by Kimura (1974), and compared with the conduction velocity obtained with the usual methods. In 25 normal subjects the F-wave velocity increased proceeding proximally, reaching the maximum values in the central tract (64.86 +/- 2.23 m/sec in ulnar nerve). In 11 patients affected by motor neurone disease and 11 patients affected by amyotrophic lateral sclerosis the F-wave velocity decreased significantly proceeding proximally and the minimum values were found in the central tract (52.51 +/- 2.15 m/sec in MND and 48.64 +/- 5.60 m/sec in ALS). We therefore suggest the use of F-wave velocity as a more complete element for precise localization of the lesion in the central segment when the motoneurone is primarily involved.", "PMID": 961378}
{"id": "pubmed23n0032_4362", "title": "[Changes of motor innervation in myasthenic muscles in relation to age (author's transl)].", "content": "The motor innervation in muscle biopsies from 45 myasthenic patients was studied by intravital staining with methylene blue. The enzymatic pattern and fibre type distribution was analysed in 12 cases. Quantitative data including the proportion of elongated motor endings and the terminal innervation ratio (TIR) of motor axons, were compared to histochemical data and to clinical data including age of patients. The incidence of elongated motor endings tends to be greater in younger patients, whereas an increased collateral ramification of motor axons occurs only after the age of 50. Small type III fibers suggesting denervation are also found in elderly patients only. The significance of these morphological differences in relation to age is discussed.", "contents": "[Changes of motor innervation in myasthenic muscles in relation to age (author's transl)]. The motor innervation in muscle biopsies from 45 myasthenic patients was studied by intravital staining with methylene blue. The enzymatic pattern and fibre type distribution was analysed in 12 cases. Quantitative data including the proportion of elongated motor endings and the terminal innervation ratio (TIR) of motor axons, were compared to histochemical data and to clinical data including age of patients. The incidence of elongated motor endings tends to be greater in younger patients, whereas an increased collateral ramification of motor axons occurs only after the age of 50. Small type III fibers suggesting denervation are also found in elderly patients only. The significance of these morphological differences in relation to age is discussed.", "PMID": 961373}
{"id": "pubmed23n0032_4363", "title": "Diazepam: intravenous infusion in the treatment of status epilepticus.", "content": "As the anticonvulsant plasma level of diazepam (DZP) after intravenous injection is maintained only for a short period, it is profitable to administer DZP as an intravenous infusion. It has, however, been claimed that DZP cannot be mixed with dextrose injection as DZP would precipitate. As this is in contrast to our clinical experience we added DZP of various brands to dextrose injections. A precipitate was in fact found in dextrose injections containing Valium and Stesolid whereas Diazepam A.L. was not only slightly cloudy. The concentration of DZP was, however, the same in all the samples and corresponded to the calculated concentration. The precipitate in the solutions with Valium and Stesolid which did not pass the filter must therefore be due to additives, most probably benzoates. On the basis of these findings and our clinical experience of this mode of administration we recommend the infusion method as one of the most effective in the acute treatment of status epilepticus.", "contents": "Diazepam: intravenous infusion in the treatment of status epilepticus. As the anticonvulsant plasma level of diazepam (DZP) after intravenous injection is maintained only for a short period, it is profitable to administer DZP as an intravenous infusion. It has, however, been claimed that DZP cannot be mixed with dextrose injection as DZP would precipitate. As this is in contrast to our clinical experience we added DZP of various brands to dextrose injections. A precipitate was in fact found in dextrose injections containing Valium and Stesolid whereas Diazepam A.L. was not only slightly cloudy. The concentration of DZP was, however, the same in all the samples and corresponded to the calculated concentration. The precipitate in the solutions with Valium and Stesolid which did not pass the filter must therefore be due to additives, most probably benzoates. On the basis of these findings and our clinical experience of this mode of administration we recommend the infusion method as one of the most effective in the acute treatment of status epilepticus.", "PMID": 961379}
{"id": "pubmed23n0032_4364", "title": "[Neuropsychological aspect of normal pressure hydrocephalus (author's transl)].", "content": "The authors report ten cases of normal pressure hydrocephalus treated by ventricular drainage. They pay particular attention to the neuropsychological and psychometric aspects and endeavour to identify the most characteristic nosological features by comparing their own clinical observations with those reported in the literature.", "contents": "[Neuropsychological aspect of normal pressure hydrocephalus (author's transl)]. The authors report ten cases of normal pressure hydrocephalus treated by ventricular drainage. They pay particular attention to the neuropsychological and psychometric aspects and endeavour to identify the most characteristic nosological features by comparing their own clinical observations with those reported in the literature.", "PMID": 961374}
{"id": "pubmed23n0032_4365", "title": "Neuropathological aspects of dementias resulting from abnormal blood and cerebrospinal fluid dynamics.", "content": "In a large autopsy series of elderly individuals, organic dementia was attributed to (pre-) senile atrophy in 52.8%, to cerebrovascular disease in 22.5%, while 13.6% were of mixed senile and vascular origin, and 1.3% showed communicating hydrocephalus with meningopathies or were of undetermined origin. A survey is given of the morphological criteria of dementia resulting from disorders of cerebral blood supply and CSF circulation. The anatomic basis of vascular dementias are: atherosclerotic encephalopathy with lacunar state or multiple infarcts; granular cortical atrophy resulting from local microcirculation disorders; hypertensive cerebrovascular disease with the common \"mixed\" cortico-subcortical type, and the rare Binswanger's subcortical type. Atypical cerebral hemorrhage in old individuals rather results from congophilic (amyloid) angiopathy than from hypertensive arteriosclerosis. Multiple infarct dementia may also result from thrombotic microangiopathy, thromboembolic disease or cerebral vasculitides. The anatomical features of dementia associated with communicating \"normal-pressure\" hydrocephalus (NPH) are meningopathy at the basis or on the convexity, and fibrosis of the choroid plexus and/or arachnoid villi of post-inflammatory or undetermined origin, and other non-specific changes (periventricular gliosis). This condition is also associated with hypertensive cerebrovascular disease and Alzheimer's disease. Cerebral biopsies in NPH as well as in other types of hydrocephalus show enlarged extracellular spaces with otherwise normal neuropil probably resulting from increased transcapillary filtration. In some cases of \"idiopathic\" NPH no causative anatomical changes are found. The relationship between cerebral tissue changes, abnormal blood and CSF dynamics in these conditions remains to be clarified.", "contents": "Neuropathological aspects of dementias resulting from abnormal blood and cerebrospinal fluid dynamics. In a large autopsy series of elderly individuals, organic dementia was attributed to (pre-) senile atrophy in 52.8%, to cerebrovascular disease in 22.5%, while 13.6% were of mixed senile and vascular origin, and 1.3% showed communicating hydrocephalus with meningopathies or were of undetermined origin. A survey is given of the morphological criteria of dementia resulting from disorders of cerebral blood supply and CSF circulation. The anatomic basis of vascular dementias are: atherosclerotic encephalopathy with lacunar state or multiple infarcts; granular cortical atrophy resulting from local microcirculation disorders; hypertensive cerebrovascular disease with the common \"mixed\" cortico-subcortical type, and the rare Binswanger's subcortical type. Atypical cerebral hemorrhage in old individuals rather results from congophilic (amyloid) angiopathy than from hypertensive arteriosclerosis. Multiple infarct dementia may also result from thrombotic microangiopathy, thromboembolic disease or cerebral vasculitides. The anatomical features of dementia associated with communicating \"normal-pressure\" hydrocephalus (NPH) are meningopathy at the basis or on the convexity, and fibrosis of the choroid plexus and/or arachnoid villi of post-inflammatory or undetermined origin, and other non-specific changes (periventricular gliosis). This condition is also associated with hypertensive cerebrovascular disease and Alzheimer's disease. Cerebral biopsies in NPH as well as in other types of hydrocephalus show enlarged extracellular spaces with otherwise normal neuropil probably resulting from increased transcapillary filtration. In some cases of \"idiopathic\" NPH no causative anatomical changes are found. The relationship between cerebral tissue changes, abnormal blood and CSF dynamics in these conditions remains to be clarified.", "PMID": 961375}
{"id": "pubmed23n0032_4366", "title": "Selenium, vitamin E and copper in multiple sclerosis.", "content": "There has been accumulation of the nutritional muscular dystrophy of the cattle in a certain western district of Finland where the prevalence of multiple sclerosis (MS) is also highest. This animal disease is due to lack of selenium (Se) and vitamin E. The Se content of whole blood was low (52.6 +/- 11.3 ng/ml) in MS patients from this high-risk area compared to the controls (68.8 +/- 11.0). The data for serum failed to confirm this tendency. All Se values appeared to be lower than international values suggested. The values for both vitamin E and copper were within the international normal range.", "contents": "Selenium, vitamin E and copper in multiple sclerosis. There has been accumulation of the nutritional muscular dystrophy of the cattle in a certain western district of Finland where the prevalence of multiple sclerosis (MS) is also highest. This animal disease is due to lack of selenium (Se) and vitamin E. The Se content of whole blood was low (52.6 +/- 11.3 ng/ml) in MS patients from this high-risk area compared to the controls (68.8 +/- 11.0). The data for serum failed to confirm this tendency. All Se values appeared to be lower than international values suggested. The values for both vitamin E and copper were within the international normal range.", "PMID": 961380}
{"id": "pubmed23n0032_4367", "title": "Neurofibrillary degeneration induced by systemic aluminum.", "content": "Neurofibrillary degeneration was induced in brain of rabbits by repeated, subcutaneous administration of soluble aluminum salts. Atomic absorption spectroscopy showed brain aluminum levels to increase from 1.1+/0.3mug/g (dry weight) in uninjected animals to concentrations ranging from 2.5 to 47.9 mug/g (dry weight). The Bielschowski silver stain revealed intracellular, argentophilic deposits, which were birefringent when stained with Congo red and viewed in polarized light. The histochemical stain morin revealed aluminum to be concentrated on nuclear chromatin. Analysis by electron microscopy showed the intra-neuronal deposits to be composed of accumulations of 100 A diameter filaments. These observations indicate that systemic aluminum crosses an apparently intact blood-brain barrier and induces neurofibrillary degeneration identical to that following direct brain injection.", "contents": "Neurofibrillary degeneration induced by systemic aluminum. Neurofibrillary degeneration was induced in brain of rabbits by repeated, subcutaneous administration of soluble aluminum salts. Atomic absorption spectroscopy showed brain aluminum levels to increase from 1.1+/0.3mug/g (dry weight) in uninjected animals to concentrations ranging from 2.5 to 47.9 mug/g (dry weight). The Bielschowski silver stain revealed intracellular, argentophilic deposits, which were birefringent when stained with Congo red and viewed in polarized light. The histochemical stain morin revealed aluminum to be concentrated on nuclear chromatin. Analysis by electron microscopy showed the intra-neuronal deposits to be composed of accumulations of 100 A diameter filaments. These observations indicate that systemic aluminum crosses an apparently intact blood-brain barrier and induces neurofibrillary degeneration identical to that following direct brain injection.", "PMID": 961381}
{"id": "pubmed23n0032_4368", "title": "Increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in Mongolian Gerbils.", "content": "Cerebral ischemia was induced by occlusion of the left common carotic artery in adult Mongolian gerbils. The period of occlusion was 3, 6, or 18 h. Horseradish peroxidase (HRP) was intravenously injected in animals with clear neurological signs 1 h release of the clip. The HRP was allowed to circulate for 5 min. Fixation was carried out by perfusion with aldehydes. Tissue, incubated for peroxidatic activity, from the left side of the brain was treated for electron microscopy. During the postischemic period enhanced permeability was demonstrated in the brains of all animals. The amount of HRP transferred into the neuropil depended on the duration of ischemia. Thus the gerbils with 18 h occlusion showed the greatest content. The cells comprising the neuropil adjacent to vessels were studied and the degree of the pathological changes described below was increased proportionally to the time period of occlusion. The intercellular spaces, often filled with peroxidase, were expanded and the astrocytes swollen, especially the endfeet. Sometimes the astrocytes were pervious to HRP. The neurons were also swollen, but to a lesser degree than the astrocytes. No endothelial cell damage was observed. Even 18 h of occlusion did not change the plasma membranes. The intercellular spaces were free of HRP from the first luminal to the first abluminal tight junction. The cytoplasm exhibited HRP-containing vesicles of various types and shapes. Some were freely situated; others were connected to the plasma membrane and then open to the vessel lumen or to the basement membrane. Since no cell damage was demonstrated, and since no HRP was diffusely dispersed in the cytoplasm it is assumed that vesicles are responsible for the enhancement of the vesicular transport that normally occurs after intravenous injection of HRP.", "contents": "Increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in Mongolian Gerbils. Cerebral ischemia was induced by occlusion of the left common carotic artery in adult Mongolian gerbils. The period of occlusion was 3, 6, or 18 h. Horseradish peroxidase (HRP) was intravenously injected in animals with clear neurological signs 1 h release of the clip. The HRP was allowed to circulate for 5 min. Fixation was carried out by perfusion with aldehydes. Tissue, incubated for peroxidatic activity, from the left side of the brain was treated for electron microscopy. During the postischemic period enhanced permeability was demonstrated in the brains of all animals. The amount of HRP transferred into the neuropil depended on the duration of ischemia. Thus the gerbils with 18 h occlusion showed the greatest content. The cells comprising the neuropil adjacent to vessels were studied and the degree of the pathological changes described below was increased proportionally to the time period of occlusion. The intercellular spaces, often filled with peroxidase, were expanded and the astrocytes swollen, especially the endfeet. Sometimes the astrocytes were pervious to HRP. The neurons were also swollen, but to a lesser degree than the astrocytes. No endothelial cell damage was observed. Even 18 h of occlusion did not change the plasma membranes. The intercellular spaces were free of HRP from the first luminal to the first abluminal tight junction. The cytoplasm exhibited HRP-containing vesicles of various types and shapes. Some were freely situated; others were connected to the plasma membrane and then open to the vessel lumen or to the basement membrane. Since no cell damage was demonstrated, and since no HRP was diffusely dispersed in the cytoplasm it is assumed that vesicles are responsible for the enhancement of the vesicular transport that normally occurs after intravenous injection of HRP.", "PMID": 961382}
{"id": "pubmed23n0032_4369", "title": "Reversible blood-brain barrier dysfunction to peroxidase after a small stab wound in the rat cerebral cortex.", "content": "Small stab wounds were made in the rat frontal lobe. The animals were injected with horseradish peroxidase intravenously at different times after the injury in order to study the extravasation of this tracer. There was a leakage of peroxidase into the brain during the first 3 days after the injury. The route of passage from the vessel lumen into the brain was through disrupted blood vessels in the injured region. Endothelial pinocytosis and formation of thin, trans-endothelial channel-like structures with or without a content of peroxidase were two other possible routes of passage across the blood vessels. Occasionally, badly damaged endothelial cells displayed a diffuse cytoplasmic distribution of peroxidase, indication a diffusion into and possibly across these injured cells. No widened tight junctions were seen. Thus, this study indicated four possible routes of passage of horseradish peroxidase across the endothelial cells: cellular gross damage with disrupture of the cells, diffusion across badly injured endothelial cells, possibly pinocytosis and formation of trans-endothelial channel-like structures. The cellular uptake of the tracer was vesicular in most neurons, astrocytes, oligodendrocytes and hematogeneous phagocytes. However, a diffuse distribution of the tracer was seen in some \"dark\" neurons near leaking vessels in the vicinity of the stab wound.", "contents": "Reversible blood-brain barrier dysfunction to peroxidase after a small stab wound in the rat cerebral cortex. Small stab wounds were made in the rat frontal lobe. The animals were injected with horseradish peroxidase intravenously at different times after the injury in order to study the extravasation of this tracer. There was a leakage of peroxidase into the brain during the first 3 days after the injury. The route of passage from the vessel lumen into the brain was through disrupted blood vessels in the injured region. Endothelial pinocytosis and formation of thin, trans-endothelial channel-like structures with or without a content of peroxidase were two other possible routes of passage across the blood vessels. Occasionally, badly damaged endothelial cells displayed a diffuse cytoplasmic distribution of peroxidase, indication a diffusion into and possibly across these injured cells. No widened tight junctions were seen. Thus, this study indicated four possible routes of passage of horseradish peroxidase across the endothelial cells: cellular gross damage with disrupture of the cells, diffusion across badly injured endothelial cells, possibly pinocytosis and formation of trans-endothelial channel-like structures. The cellular uptake of the tracer was vesicular in most neurons, astrocytes, oligodendrocytes and hematogeneous phagocytes. However, a diffuse distribution of the tracer was seen in some \"dark\" neurons near leaking vessels in the vicinity of the stab wound.", "PMID": 961383}
{"id": "pubmed23n0032_4370", "title": "Experimental herpes simplex virus infection in the immature mouse brain.", "content": "Mice of different ages during the postnatal development were injected intracerebrally with herpes simplex virus, type 1. Ultrastructurally, virus particles were demonstrated in the undiffereniated neuroectodermal cells of the subventricular zone and in the external layer of the cerebellum. Virus particles were also seen in endothelial cells of immature animals. The virus gave rise to an acute infection; which caused more extensive necrosis and bleeding in the cerebrum and cerebellum of the immature than the older mice.", "contents": "Experimental herpes simplex virus infection in the immature mouse brain. Mice of different ages during the postnatal development were injected intracerebrally with herpes simplex virus, type 1. Ultrastructurally, virus particles were demonstrated in the undiffereniated neuroectodermal cells of the subventricular zone and in the external layer of the cerebellum. Virus particles were also seen in endothelial cells of immature animals. The virus gave rise to an acute infection; which caused more extensive necrosis and bleeding in the cerebrum and cerebellum of the immature than the older mice.", "PMID": 961384}
{"id": "pubmed23n0032_4371", "title": "Third ventricular chromophobe adenoma with normal adenohypophysis.", "content": "An unusual case of chromophobe adenoma occurring in the third ventricle is reported. Ventriculography revealed a filling defect in the third ventricle and at autopsy the tumour was found in the posterior part of the third ventricle. No tumour was detected in the pituitary gland.", "contents": "Third ventricular chromophobe adenoma with normal adenohypophysis. An unusual case of chromophobe adenoma occurring in the third ventricle is reported. Ventriculography revealed a filling defect in the third ventricle and at autopsy the tumour was found in the posterior part of the third ventricle. No tumour was detected in the pituitary gland.", "PMID": 961385}
{"id": "pubmed23n0032_4372", "title": "Malignant meningioma with liver metastases and hypoglycaemia. A case report.", "content": "The history, clinical course and pathological findings are reported of a patient who developed a recurring, malignant meningioma. Widespread extracranial metastases occurred, particularly in the liver. Hypoglycaemia was a prominent feature during the late course of the disease. The influence of the histological type of meningioma and the effects of surgical interventions on the development of extracranial metastases are discussed together with the relationship of the tumour to hypoglycaemia.", "contents": "Malignant meningioma with liver metastases and hypoglycaemia. A case report. The history, clinical course and pathological findings are reported of a patient who developed a recurring, malignant meningioma. Widespread extracranial metastases occurred, particularly in the liver. Hypoglycaemia was a prominent feature during the late course of the disease. The influence of the histological type of meningioma and the effects of surgical interventions on the development of extracranial metastases are discussed together with the relationship of the tumour to hypoglycaemia.", "PMID": 961386}
{"id": "pubmed23n0032_4373", "title": "Osteoarthritis at the base of the thumb. The concept of monoarticular reconstruction.", "content": "Osteoarthritic affection of the base of the thumb involving 148 hands has been studied. Isolated osteoarthritis of the trapezio-metacarpal joint was found to be the largest group including a total of 111 hands. Reconstruction of this particular joint by a new technique is described and long-term results reported. A small series of isolated osteoarthritis of the trapezio-scaphoid joint is presented and reconstruction by replacing the affected articular surface of the carpal scaphoid is described. Osteoarthritic involvement of more than one of the articular surfaces of the trapezium was found in a group of 31 hands. In all of them the trapezio-metacarpal joint was predominantly affected. The rationale for monoarticular reconstruction of the trapezio-metacarpal joint, regardless of the extent of involvement, is discussed in detail.", "contents": "Osteoarthritis at the base of the thumb. The concept of monoarticular reconstruction. Osteoarthritic affection of the base of the thumb involving 148 hands has been studied. Isolated osteoarthritis of the trapezio-metacarpal joint was found to be the largest group including a total of 111 hands. Reconstruction of this particular joint by a new technique is described and long-term results reported. A small series of isolated osteoarthritis of the trapezio-scaphoid joint is presented and reconstruction by replacing the affected articular surface of the carpal scaphoid is described. Osteoarthritic involvement of more than one of the articular surfaces of the trapezium was found in a group of 31 hands. In all of them the trapezio-metacarpal joint was predominantly affected. The rationale for monoarticular reconstruction of the trapezio-metacarpal joint, regardless of the extent of involvement, is discussed in detail.", "PMID": 961387}
{"id": "pubmed23n0032_4374", "title": "Supracondylar fractures of the humerus in children. A follow-up with particular reference to late results after severely displaced fractures.", "content": "A follow-up of 73 cases out of a 10-year material of 90 children with supracondylar fractures of the humerus is described. Forty-three children had fractures with gross displacement, whereas 30 children had undisplaced or slightly displaced fractures. This last group all achieved excellent results after simple conservative treatment. Twenty-three cases among the group with severe displacement were treated with vertical extension, 17 cases with one or more attempts at manipulative reduction and plaster fixation, and three with operation using Kirschner wires. The functional results were approximately the same in the first two groups and were satisfactory in 85-100%. The results with varus derformity were better in the group treated by extension (82% as opposed to 50% in the group treated by manual reposition). We conclude that extension in a vertical direction is the most suitable method of treatment of these often severely displaced fractures.", "contents": "Supracondylar fractures of the humerus in children. A follow-up with particular reference to late results after severely displaced fractures. A follow-up of 73 cases out of a 10-year material of 90 children with supracondylar fractures of the humerus is described. Forty-three children had fractures with gross displacement, whereas 30 children had undisplaced or slightly displaced fractures. This last group all achieved excellent results after simple conservative treatment. Twenty-three cases among the group with severe displacement were treated with vertical extension, 17 cases with one or more attempts at manipulative reduction and plaster fixation, and three with operation using Kirschner wires. The functional results were approximately the same in the first two groups and were satisfactory in 85-100%. The results with varus derformity were better in the group treated by extension (82% as opposed to 50% in the group treated by manual reposition). We conclude that extension in a vertical direction is the most suitable method of treatment of these often severely displaced fractures.", "PMID": 961390}
{"id": "pubmed23n0032_4375", "title": "A study of painful shoulder in welders.", "content": "By means of clinical examination, soft tissue radiography and quantitative electromyography, the effect of heavy industrial work was evaluated on welders at a shipyard. The study revealed that the older workers with shoulder pain have a chronic tendinitis of the rotator cuff. The supraspinatus muscle was shown to be consistently fatigued during overhead welding. It is believed that this is an important factor in the aetiology of the shoulder pain commonly occurring in older welders.", "contents": "A study of painful shoulder in welders. By means of clinical examination, soft tissue radiography and quantitative electromyography, the effect of heavy industrial work was evaluated on welders at a shipyard. The study revealed that the older workers with shoulder pain have a chronic tendinitis of the rotator cuff. The supraspinatus muscle was shown to be consistently fatigued during overhead welding. It is believed that this is an important factor in the aetiology of the shoulder pain commonly occurring in older welders.", "PMID": 961391}
{"id": "pubmed23n0032_4376", "title": "Intraspinal tumours in the first two decades of life. Clinical and radiological features.", "content": "Twenty-eight patients with spinal tumours (11 malignant and 17 benign) detected in their first two decades of life have been reviewed. Twenty-six of the 28 patients had neurological disturbances, and a significant difference in the duration of debut symptoms in the benign and malignant groups could be demonstrated. X-ray survey was positive in 11 cases in the benign group and in three cases in the malignant group.", "contents": "Intraspinal tumours in the first two decades of life. Clinical and radiological features. Twenty-eight patients with spinal tumours (11 malignant and 17 benign) detected in their first two decades of life have been reviewed. Twenty-six of the 28 patients had neurological disturbances, and a significant difference in the duration of debut symptoms in the benign and malignant groups could be demonstrated. X-ray survey was positive in 11 cases in the benign group and in three cases in the malignant group.", "PMID": 961392}
{"id": "pubmed23n0032_4377", "title": "Operative treatment of scoliosis with the Harrington instrumentation technique.", "content": "Preliminary results of treatment of scoliosis with the Harrington instrumentation technique in 80 patients are presented. The curve correction at operation averaged 43.4% with the best results being achieved in idiopathic single curves (49.3%). Most of the patients had been treated conservatively for a long time prior to operation, and the curves were rather stiff. The initial loss of correction was 3.2 degrees, and the overall loss at 2 years postoperatively averaged 6.5 degrees in 28 patients. Complications occurred in 22.5% of the patients, most often at the upper hook site. Serious complications were rare. It is concluded that the Harrington instrumentation technique is an effective means of treatment of scoliosis.", "contents": "Operative treatment of scoliosis with the Harrington instrumentation technique. Preliminary results of treatment of scoliosis with the Harrington instrumentation technique in 80 patients are presented. The curve correction at operation averaged 43.4% with the best results being achieved in idiopathic single curves (49.3%). Most of the patients had been treated conservatively for a long time prior to operation, and the curves were rather stiff. The initial loss of correction was 3.2 degrees, and the overall loss at 2 years postoperatively averaged 6.5 degrees in 28 patients. Complications occurred in 22.5% of the patients, most often at the upper hook site. Serious complications were rare. It is concluded that the Harrington instrumentation technique is an effective means of treatment of scoliosis.", "PMID": 961393}
{"id": "pubmed23n0032_4378", "title": "The CE angle of normal hips.", "content": "The Center-Edge angle (CE angle) of Wiberg was measured in normal hips in patients between 8 and 75 years of age. Males and females were recorded separately. Means and standard deviations are presented here. The angle increased with growth up to adult age, and then only slowly. In children only values below 15 degrees can be classified as abnormal.", "contents": "The CE angle of normal hips. The Center-Edge angle (CE angle) of Wiberg was measured in normal hips in patients between 8 and 75 years of age. Males and females were recorded separately. Means and standard deviations are presented here. The angle increased with growth up to adult age, and then only slowly. In children only values below 15 degrees can be classified as abnormal.", "PMID": 961394}
{"id": "pubmed23n0032_4379", "title": "Compression and the osteotomy gap.", "content": "The existence of a clearly visible osteotomy gap by optimal roentgenography even in cases where high compression had been applied is exemplified. One case of fracture of the osteosynthesis material used in a medial displacement osteotomy is illustrated. It is suggested that this and similar accidents are sometimes consequences of imperfect surface contact at the operation, visible only by central beam radiography.", "contents": "Compression and the osteotomy gap. The existence of a clearly visible osteotomy gap by optimal roentgenography even in cases where high compression had been applied is exemplified. One case of fracture of the osteosynthesis material used in a medial displacement osteotomy is illustrated. It is suggested that this and similar accidents are sometimes consequences of imperfect surface contact at the operation, visible only by central beam radiography.", "PMID": 961395}
{"id": "pubmed23n0032_4380", "title": "Pertrochanteric fractures of the femur treated with a K\u00fcntscher trochanter nail.", "content": "Thirty-nine patients with pertrochanteric fractures were treated with the K\u00fcntscher trochanter nail. Six were males and 33 females. Their mean age was 76 years. Excellent or good results of treatment were recorded in 25 cases, and good union of the fracture was noted in 19 patients within 4 months and in 15 cases as early as 2 months after the operation. The primary mortality rate was of the same order as with other methods of nailing, 15% in this series. The operation is easy and short, and is suitable at least for stable pertrochanteric fractures of elderly patients.", "contents": "Pertrochanteric fractures of the femur treated with a K\u00fcntscher trochanter nail. Thirty-nine patients with pertrochanteric fractures were treated with the K\u00fcntscher trochanter nail. Six were males and 33 females. Their mean age was 76 years. Excellent or good results of treatment were recorded in 25 cases, and good union of the fracture was noted in 19 patients within 4 months and in 15 cases as early as 2 months after the operation. The primary mortality rate was of the same order as with other methods of nailing, 15% in this series. The operation is easy and short, and is suitable at least for stable pertrochanteric fractures of elderly patients.", "PMID": 961396}
{"id": "pubmed23n0032_4381", "title": "A new design of the Christiansen endoprosthesis. Report of two cases in which separation of the two components of the prosthetic head occurred.", "content": "Primary prosthetic replacement in elderly patients with displaced intracapsular fracture of the femoral neck has been used during the last few years with encouraging early results. The prosthetic device preferred has been Christiansen's endoprosthesis. During the past year a new type with separable plastic head and metallic cap has been used. This modification has resulted in the occurrence of a previously unknown complication, a separation of these two parts, necessitating reoperation and insertion of a new head piece. The possible mechanisms causing this separation are discussed.", "contents": "A new design of the Christiansen endoprosthesis. Report of two cases in which separation of the two components of the prosthetic head occurred. Primary prosthetic replacement in elderly patients with displaced intracapsular fracture of the femoral neck has been used during the last few years with encouraging early results. The prosthetic device preferred has been Christiansen's endoprosthesis. During the past year a new type with separable plastic head and metallic cap has been used. This modification has resulted in the occurrence of a previously unknown complication, a separation of these two parts, necessitating reoperation and insertion of a new head piece. The possible mechanisms causing this separation are discussed.", "PMID": 961397}
{"id": "pubmed23n0032_4382", "title": "Congenital bifid femur.", "content": "Two identical and unusual cases of congenital bifid femur are described. Both cases were successfully rehabilitated after simple surgery and the fitting of a suitable prosthesis. The patients are now able to walk without any form of external support.", "contents": "Congenital bifid femur. Two identical and unusual cases of congenital bifid femur are described. Both cases were successfully rehabilitated after simple surgery and the fitting of a suitable prosthesis. The patients are now able to walk without any form of external support.", "PMID": 961398}
{"id": "pubmed23n0032_4383", "title": "Experience with knee synovectomy in Norwich, 1964-1973.", "content": "Thirty-six patients (43 knees) who underwent anterior synovectomy for rheumatoid disease between 1964 and 1973 have been personaly reviewed. The notes and X-rays of a further seven patients (10 knees) have been examined. Twenty-five per cent of the knees were failures at the time of review, but only two were failures from the outset. The indications for the operation and also the contraindications are described and evaluated. It is concluded that: 1. In the rheumatoid knee, anterior synovectomy is a pain relieving operation. 2. The indications are wider than classically stated, and can include knees with full thickness cartilage destruction, and flexion deformities up to 20 degrees. 3. The majority of knees fail because of secondary degenerative change, and this is manifested by pain rather than stiffness. 4. Postoperative manipulation does not prejudice the final result. 5. Associated popliteal cysts (five in this series) can be cured by anterior synovectomy.", "contents": "Experience with knee synovectomy in Norwich, 1964-1973. Thirty-six patients (43 knees) who underwent anterior synovectomy for rheumatoid disease between 1964 and 1973 have been personaly reviewed. The notes and X-rays of a further seven patients (10 knees) have been examined. Twenty-five per cent of the knees were failures at the time of review, but only two were failures from the outset. The indications for the operation and also the contraindications are described and evaluated. It is concluded that: 1. In the rheumatoid knee, anterior synovectomy is a pain relieving operation. 2. The indications are wider than classically stated, and can include knees with full thickness cartilage destruction, and flexion deformities up to 20 degrees. 3. The majority of knees fail because of secondary degenerative change, and this is manifested by pain rather than stiffness. 4. Postoperative manipulation does not prejudice the final result. 5. Associated popliteal cysts (five in this series) can be cured by anterior synovectomy.", "PMID": 961399}
{"id": "pubmed23n0032_4384", "title": "Patellectomy after fracture. Long-term follow-up results with special reference to functional disability.", "content": "Twenty-eight out of a group of 38 patients, who had undergone a patellectomy during the period 1950-70, were investigated clinically and radiologically. The results were compared with the subjective symptoms of the patients to see if there was a correlation. The follow-up period was 7.4 years on average. A good subjective result was reported by only six patients. The predominant subjective symptoms were weakness of the limb and pain on movement and/or exertion. The most usual findings were atrophy of the quadriceps muscle, crepitation, and palpation tenderness. The muscular power of the quadriceps was found to be greater than or equal to 75 per cent of the power of the intact knee in only seven cases (25 per cent).", "contents": "Patellectomy after fracture. Long-term follow-up results with special reference to functional disability. Twenty-eight out of a group of 38 patients, who had undergone a patellectomy during the period 1950-70, were investigated clinically and radiologically. The results were compared with the subjective symptoms of the patients to see if there was a correlation. The follow-up period was 7.4 years on average. A good subjective result was reported by only six patients. The predominant subjective symptoms were weakness of the limb and pain on movement and/or exertion. The most usual findings were atrophy of the quadriceps muscle, crepitation, and palpation tenderness. The muscular power of the quadriceps was found to be greater than or equal to 75 per cent of the power of the intact knee in only seven cases (25 per cent).", "PMID": 961401}
{"id": "pubmed23n0032_4385", "title": "Fractures of the tibia in children.", "content": "A total of 102 children, aged 1-15 years, treated for fissures, infractions, and fractures of the tibia were studied to elucidate the influence of age, type of fracture, and mechanism of trauma upon the course of union. In addition, an assessment was made of the possibilities the child has of correcting deformities of the diaphysis during continued growth. Eight-five of the children were followed up clinically and radiologically. The time taken for union to occur increased with increasing age. The \"high energy\" injuries were found to be more apt to cause transverse and comminuted fractures, with injury to the skin, than the \"low energy\" fractures. At the time of union, 25 patients had angular deformities. The mean correction of this deformity up to the time of follow-up was only 10 per cent. The tendency to correct the deformity ceased 18 months after the accident, and was independent of the child's age at the time of the accident.", "contents": "Fractures of the tibia in children. A total of 102 children, aged 1-15 years, treated for fissures, infractions, and fractures of the tibia were studied to elucidate the influence of age, type of fracture, and mechanism of trauma upon the course of union. In addition, an assessment was made of the possibilities the child has of correcting deformities of the diaphysis during continued growth. Eight-five of the children were followed up clinically and radiologically. The time taken for union to occur increased with increasing age. The \"high energy\" injuries were found to be more apt to cause transverse and comminuted fractures, with injury to the skin, than the \"low energy\" fractures. At the time of union, 25 patients had angular deformities. The mean correction of this deformity up to the time of follow-up was only 10 per cent. The tendency to correct the deformity ceased 18 months after the accident, and was independent of the child's age at the time of the accident.", "PMID": 961402}
{"id": "pubmed23n0032_4386", "title": "Conservative treatment to Achilles tendon rupture. A follow-up study of 14 cases.", "content": "A follow-up study of 14 patients with Achilles tendon rupture is reported. The patients were treated by conservative methods, i.e. with immobilization in a plaster cast for 8 weeks with the foot in the relaxed equinus position. Solid healing of the tendon was obtained in 13 cases. As assessed by the patients, the end result was unsatisfactory in two, fairly satisfactory in one and fully satisfactory in 11 cases.", "contents": "Conservative treatment to Achilles tendon rupture. A follow-up study of 14 cases. A follow-up study of 14 patients with Achilles tendon rupture is reported. The patients were treated by conservative methods, i.e. with immobilization in a plaster cast for 8 weeks with the foot in the relaxed equinus position. Solid healing of the tendon was obtained in 13 cases. As assessed by the patients, the end result was unsatisfactory in two, fairly satisfactory in one and fully satisfactory in 11 cases.", "PMID": 961403}
{"id": "pubmed23n0032_4387", "title": "Decompression with the aid of insoles in the treatment of diabetic neuropathic ulcers.", "content": "Thirty-seven out of 38 neuropathic ulcers in 21 diabetic patients healed during relief of external pressure obtained by properly fitted interchangeable insoles. The time required for healing was 1 to 12 months (mean 3.6 months). The presence of mild occlussive arterial disease did not influence the course of healing. Gross infection, which occurred in eight patients could be controlled by immobilization, antibiotics and, in the presence of pus, by radical surgical drainage.", "contents": "Decompression with the aid of insoles in the treatment of diabetic neuropathic ulcers. Thirty-seven out of 38 neuropathic ulcers in 21 diabetic patients healed during relief of external pressure obtained by properly fitted interchangeable insoles. The time required for healing was 1 to 12 months (mean 3.6 months). The presence of mild occlussive arterial disease did not influence the course of healing. Gross infection, which occurred in eight patients could be controlled by immobilization, antibiotics and, in the presence of pus, by radical surgical drainage.", "PMID": 961405}
{"id": "pubmed23n0032_4388", "title": "Bone mineral losses in alcoholics.", "content": "Bone mineral measurements were performed at five skeletal sites in seven alcoholics. The mean annual loss of bone mineral in alcoholics was about 2 per cent higher than that of controls.", "contents": "Bone mineral losses in alcoholics. Bone mineral measurements were performed at five skeletal sites in seven alcoholics. The mean annual loss of bone mineral in alcoholics was about 2 per cent higher than that of controls.", "PMID": 961406}
{"id": "pubmed23n0032_4389", "title": "Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea.", "content": "We have investigated small intestinal biopsies from children with coeliac disease, acute gastroenteritis, failure to thrive and giardiasis, to find out if a high intraepithelial lymphocyte count is a feature specific to coeliac disease, or whether it is always associated with partial or subtotal villous atrophy. The results indicate that the normal range for childrens' intraepithelial lymphocyte counts is similar to that for adults (around 6-40 lymphocytes per 100 epithelial cells); that counts are high in coeliac disease, but also in some children with giardiasis or with failure to thrive in whom the jejunal biopsy appears otherwise normal; and that intraepithelial lymphocyte counts are normal in acute gastroenteritis even when there is partial villous atrophy with increased lamina propria lymphoid cell infiltrate. Thus, this measurement of small intestinal lymphocyte infiltration may be of diagnostic value is differentiating the diarrhoea of food intolerance from infectious diarrhoeas in young children.", "contents": "Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea. We have investigated small intestinal biopsies from children with coeliac disease, acute gastroenteritis, failure to thrive and giardiasis, to find out if a high intraepithelial lymphocyte count is a feature specific to coeliac disease, or whether it is always associated with partial or subtotal villous atrophy. The results indicate that the normal range for childrens' intraepithelial lymphocyte counts is similar to that for adults (around 6-40 lymphocytes per 100 epithelial cells); that counts are high in coeliac disease, but also in some children with giardiasis or with failure to thrive in whom the jejunal biopsy appears otherwise normal; and that intraepithelial lymphocyte counts are normal in acute gastroenteritis even when there is partial villous atrophy with increased lamina propria lymphoid cell infiltrate. Thus, this measurement of small intestinal lymphocyte infiltration may be of diagnostic value is differentiating the diarrhoea of food intolerance from infectious diarrhoeas in young children.", "PMID": 961407}
{"id": "pubmed23n0032_4390", "title": "Growth hormone and somatomedin A in girls with adolescent idiopathic scoliosis.", "content": "Girls with adolescent idiopathic scoliosis are taller than nonscoliotic girls of the same age. This observation may be related to factors regulating longitudinal growth. Plasma growth hormone was determined in a group of scoliotic girls by double antibody radioimmunoassay under the following conditions: 1) Insulin induced hypoglycemia, 2) glucose tolerance test, 3) exercise. Somatomedin A was determined by a method based on the ability of serum to stimulate the incorporation of radioactive sulphate in embryonic chick cartilage. The results were compared with those obtained in a control group of healthy nonscoliotic girls of comparable age. After overnight fasting and after at least one hour's rest the basal growth hormone level was 9.8+/-11.1 (+/-S.D.) ng/ml in the scoliotic girls (n=48) and 2.2+/-1.1 ng/ml in the controls (n=15). This difference is significant. In the hypoglycemia test the peak growth hormone level tended to be higher in the scoliotic girls but the difference is not significant. In the exercise test the maximal value was reached at different times in the two groups: at 20 min after start of the exercise in the scoliotic girls (n=14, 17.3+/-11.8 ng/ml) and at 40 min in the controls (n=9, 16.0+/-6.6 ng/ml). In the glucose tolerance test the growth hormone level was suppressed in both groups but the mean values tended to be higher during the first 120 min in the scoliotic girls. The serum somatomedin levels were higher in the group of scoliotic girls (n=19, 1.13+/-0.17 U/ml) than in the controls (n=14, 0.88+/-0.16 U/ml) and the difference is significant. The results obtained are difficult to interpret but suggest that growth hormone secretion is higher in girls with adolescent idiopathic scoliosis than in healthy girls of comparable age.", "contents": "Growth hormone and somatomedin A in girls with adolescent idiopathic scoliosis. Girls with adolescent idiopathic scoliosis are taller than nonscoliotic girls of the same age. This observation may be related to factors regulating longitudinal growth. Plasma growth hormone was determined in a group of scoliotic girls by double antibody radioimmunoassay under the following conditions: 1) Insulin induced hypoglycemia, 2) glucose tolerance test, 3) exercise. Somatomedin A was determined by a method based on the ability of serum to stimulate the incorporation of radioactive sulphate in embryonic chick cartilage. The results were compared with those obtained in a control group of healthy nonscoliotic girls of comparable age. After overnight fasting and after at least one hour's rest the basal growth hormone level was 9.8+/-11.1 (+/-S.D.) ng/ml in the scoliotic girls (n=48) and 2.2+/-1.1 ng/ml in the controls (n=15). This difference is significant. In the hypoglycemia test the peak growth hormone level tended to be higher in the scoliotic girls but the difference is not significant. In the exercise test the maximal value was reached at different times in the two groups: at 20 min after start of the exercise in the scoliotic girls (n=14, 17.3+/-11.8 ng/ml) and at 40 min in the controls (n=9, 16.0+/-6.6 ng/ml). In the glucose tolerance test the growth hormone level was suppressed in both groups but the mean values tended to be higher during the first 120 min in the scoliotic girls. The serum somatomedin levels were higher in the group of scoliotic girls (n=19, 1.13+/-0.17 U/ml) than in the controls (n=14, 0.88+/-0.16 U/ml) and the difference is significant. The results obtained are difficult to interpret but suggest that growth hormone secretion is higher in girls with adolescent idiopathic scoliosis than in healthy girls of comparable age.", "PMID": 961408}
{"id": "pubmed23n0032_4391", "title": "Blood flow and venous distensibility in the upper and lower extremities of newborn infants.", "content": "The electrocapacitance plethysmograph was utilized to measure peripheral blood flow and venous distensibility in 17 newborn infants and 20 adults. Measurements were made in the upper and lower extremities in each subject under identical environmental conditions. Blood flow in the forearm and calf were found to be significantly higher in infants than adults. In infants there were no significant differences in the blood flow between the upper and lower extremities. In contrast, in the adults, the blood flow was significantly higher in the upper than in the lower extremities. Similarly, venous distensibility was observed to be higher in infants than in adults. While no significant differences were observed in the venous distensibility between the upper and lower extremities in infants, the venous distensibility was found to be higher in the forearm than in the leg in adults.", "contents": "Blood flow and venous distensibility in the upper and lower extremities of newborn infants. The electrocapacitance plethysmograph was utilized to measure peripheral blood flow and venous distensibility in 17 newborn infants and 20 adults. Measurements were made in the upper and lower extremities in each subject under identical environmental conditions. Blood flow in the forearm and calf were found to be significantly higher in infants than adults. In infants there were no significant differences in the blood flow between the upper and lower extremities. In contrast, in the adults, the blood flow was significantly higher in the upper than in the lower extremities. Similarly, venous distensibility was observed to be higher in infants than in adults. While no significant differences were observed in the venous distensibility between the upper and lower extremities in infants, the venous distensibility was found to be higher in the forearm than in the leg in adults.", "PMID": 961409}
{"id": "pubmed23n0032_4392", "title": "Significance of obstetric factors in prognosis of low  birthweight children.", "content": "Obstetric factors were studied in an unselected series of mothers of LBW-children and compared with those in a corresponding control series. Both groups consisted of 161 women with single pregnancies and 16 with twin pregnancies. Of the 188 LBW-children, 14% were twins. Of the single infants, 42% were appropriate for gestational age (AGA), i.e. within the 10th to 90th percentile according to Swedish curves for intrauterine growth, and 58% small for gestational age (SGA). The commonest causes of low birthweight (LBW) in the AGA-group were bleeding (34%) and premature rupture of the membranes (28%). In the SGA-group toxaemia was found in 15%, but in 50% of the SGA-group no cause of LBW was demonstrable. In this latter group the average placental weight was low. The mortality was noted and 89% of the living children were examined neurologically at 5 years of age. The prognosis of the child depended on the duration of pregnancy. Bleeding during pregnancy was a prognostically unfavourable sign, but even serious bleeding complications did not exclude a normal development of the child. The prognosis was best for those infants in whom no cause of LBW was demonstrable.", "contents": "Significance of obstetric factors in prognosis of low  birthweight children. Obstetric factors were studied in an unselected series of mothers of LBW-children and compared with those in a corresponding control series. Both groups consisted of 161 women with single pregnancies and 16 with twin pregnancies. Of the 188 LBW-children, 14% were twins. Of the single infants, 42% were appropriate for gestational age (AGA), i.e. within the 10th to 90th percentile according to Swedish curves for intrauterine growth, and 58% small for gestational age (SGA). The commonest causes of low birthweight (LBW) in the AGA-group were bleeding (34%) and premature rupture of the membranes (28%). In the SGA-group toxaemia was found in 15%, but in 50% of the SGA-group no cause of LBW was demonstrable. In this latter group the average placental weight was low. The mortality was noted and 89% of the living children were examined neurologically at 5 years of age. The prognosis of the child depended on the duration of pregnancy. Bleeding during pregnancy was a prognostically unfavourable sign, but even serious bleeding complications did not exclude a normal development of the child. The prognosis was best for those infants in whom no cause of LBW was demonstrable.", "PMID": 961410}
{"id": "pubmed23n0032_4393", "title": "Atopic allergy and immunoglobulins in children with adenoids and recurrent otitis media.", "content": "From 274 adenoidectomized children 1-16 years of age two groups were selected: children with a history of recurrent otitis media and/or otosalpingitis (middle ear effusion) and those in which nasal obstruction was the main symptom. In all, 154 children were subjected to an allergy investigation including history, skin tests, eye tests, determination of IgE and RAST, blood eosinophils and immunoglobulins G, A and M. No increased incidence of immunoglobulin deficiency was found in either group in comparison with unselected children. A family history of atopic disease and/or otitis media was highly overrepresented, especially in the children with recurrent otitis media as the reason for the adenoidectomy. Atopic diseases had occurred in 24.4% of the children. Furthermore, in both groups there was a high incidence of positive skin and eye tests. An increased incidence of eosinophila, positive RAST tests and elevated IgE levels were also found. In all, 39.6% of the children had two or more laboratory findings characteristic of the atopic state but no significant difference was found between the two groups. Possible connections between atopic allergy and recurrent otitis media are discussed.", "contents": "Atopic allergy and immunoglobulins in children with adenoids and recurrent otitis media. From 274 adenoidectomized children 1-16 years of age two groups were selected: children with a history of recurrent otitis media and/or otosalpingitis (middle ear effusion) and those in which nasal obstruction was the main symptom. In all, 154 children were subjected to an allergy investigation including history, skin tests, eye tests, determination of IgE and RAST, blood eosinophils and immunoglobulins G, A and M. No increased incidence of immunoglobulin deficiency was found in either group in comparison with unselected children. A family history of atopic disease and/or otitis media was highly overrepresented, especially in the children with recurrent otitis media as the reason for the adenoidectomy. Atopic diseases had occurred in 24.4% of the children. Furthermore, in both groups there was a high incidence of positive skin and eye tests. An increased incidence of eosinophila, positive RAST tests and elevated IgE levels were also found. In all, 39.6% of the children had two or more laboratory findings characteristic of the atopic state but no significant difference was found between the two groups. Possible connections between atopic allergy and recurrent otitis media are discussed.", "PMID": 961411}
{"id": "pubmed23n0032_4394", "title": "Stool bacterial aerobic overgrowth in the small intestine of children with acute diarrhoea.", "content": "The aerobic flora of 2 groups of children (normal and with malnutrition) with acute diarrhoea was studied, by intubation of the upper and middle small intestine and by stool culture. All the 27 children studied presented bacterial concentrations of 10(5) germs/ml at one or both levels studied. In 9 cases enteropathogen bacteria e were isolated from stools, and in 6 of these they were also found in the small intestine. The results show the elevated incidence of overgrowth of the small intestinal aerobic flora in children with acute diarrhoea. This fact is mentioned as another etiological factor to be taken into consideration in this pathology.", "contents": "Stool bacterial aerobic overgrowth in the small intestine of children with acute diarrhoea. The aerobic flora of 2 groups of children (normal and with malnutrition) with acute diarrhoea was studied, by intubation of the upper and middle small intestine and by stool culture. All the 27 children studied presented bacterial concentrations of 10(5) germs/ml at one or both levels studied. In 9 cases enteropathogen bacteria e were isolated from stools, and in 6 of these they were also found in the small intestine. The results show the elevated incidence of overgrowth of the small intestinal aerobic flora in children with acute diarrhoea. This fact is mentioned as another etiological factor to be taken into consideration in this pathology.", "PMID": 961412}
{"id": "pubmed23n0032_4395", "title": "Epidemiology of Cornelia de Lange's syndrome.", "content": "A prevalence investigation of Cornelia de Lange's syndrome in Denmark is presented. The patients were traced by screening all institutional mentally retarded patients, patients in schools and kindergartens for imbecile patients and finally by getting information on Cornelia de Lange patients known to pediatric departments. In this way 24 patients, 10 men and 14 women, were found. This amounts to a population prevalence of 0.5/100 000. Clinical data, histories of the patients and genealogical data are presented by means of tables. The eldest patient was 49 years old, but 75% of the patients were younger than 20 years. Two of the probands were sibs. Another 2 sibs were registered as mentally retarded without specific syndromes. One case of consanguinity among parents was found. The mode of ascertainment is discussed and it is concluded that the present investigation presents a minimum prevalence figure. Four patients are presented who for various reasons were not available during the prevalence investigation proper. A prevalence figure of 0.6/100 000 is found if these 4 patients are included in the calculations. One of the last mentioned patients represented a familial case. The patient in question was a girl with a younger half-brother, the mother in common, both children being very typical cases of Cornella de Lange's syndrome.", "contents": "Epidemiology of Cornelia de Lange's syndrome. A prevalence investigation of Cornelia de Lange's syndrome in Denmark is presented. The patients were traced by screening all institutional mentally retarded patients, patients in schools and kindergartens for imbecile patients and finally by getting information on Cornelia de Lange patients known to pediatric departments. In this way 24 patients, 10 men and 14 women, were found. This amounts to a population prevalence of 0.5/100 000. Clinical data, histories of the patients and genealogical data are presented by means of tables. The eldest patient was 49 years old, but 75% of the patients were younger than 20 years. Two of the probands were sibs. Another 2 sibs were registered as mentally retarded without specific syndromes. One case of consanguinity among parents was found. The mode of ascertainment is discussed and it is concluded that the present investigation presents a minimum prevalence figure. Four patients are presented who for various reasons were not available during the prevalence investigation proper. A prevalence figure of 0.6/100 000 is found if these 4 patients are included in the calculations. One of the last mentioned patients represented a familial case. The patient in question was a girl with a younger half-brother, the mother in common, both children being very typical cases of Cornella de Lange's syndrome.", "PMID": 961413}
{"id": "pubmed23n0032_4396", "title": "Intestinal enterokinase deficiency.", "content": "Deficiency of intestinal enterokinase results in failure to thrive, diarrhoea, anaemia, hypoproteinaemia and oedema. A case arising in the neonatal period is described, in which several of the characteristic features were lacking. Difficulties encountered in the diagnosis and methods of assay are discussed.", "contents": "Intestinal enterokinase deficiency. Deficiency of intestinal enterokinase results in failure to thrive, diarrhoea, anaemia, hypoproteinaemia and oedema. A case arising in the neonatal period is described, in which several of the characteristic features were lacking. Difficulties encountered in the diagnosis and methods of assay are discussed.", "PMID": 961414}
{"id": "pubmed23n0032_4397", "title": "Cells containing Langerhans cell granules in human lymph nodes of \"atypical hyperplasia\" with fatal outcome and leukemic reticuloendotheliosis.", "content": "Langerhans cell granules could be found in atypical histiocytes in lymph nodes of three patients with \"atypical hyperplasia\" with fatal outcome and one patient with leukemic reticuloendotheliosis. These atypical histiocytes might be derived from immature mesenchymal cells in lymph nodes and the Langerhans cell granules might be induced in these cells by a particular condition. Only one of the Langerhans cell granules seemingly associated with the plasma membrane could be observed in these atypical histiocytes and all of the granules were seen within the cytoplasm. Quite a number of Langerhans cell granules were located near the Golgi apparatus. Several atypical granules very similar to the Langerhans cell granules could also be observed in these atypical histiocytes. These Langerhans cell granules were assumed to be directly derived from the Golgi apparatus and/or derived from the atypical granules which were secreted from the Golgi apparatus. The relationship between the Langerhans cell granules and the microtubules must also be considered, because Langerhans cell granules were found near the centrioles and microtubules.", "contents": "Cells containing Langerhans cell granules in human lymph nodes of \"atypical hyperplasia\" with fatal outcome and leukemic reticuloendotheliosis. Langerhans cell granules could be found in atypical histiocytes in lymph nodes of three patients with \"atypical hyperplasia\" with fatal outcome and one patient with leukemic reticuloendotheliosis. These atypical histiocytes might be derived from immature mesenchymal cells in lymph nodes and the Langerhans cell granules might be induced in these cells by a particular condition. Only one of the Langerhans cell granules seemingly associated with the plasma membrane could be observed in these atypical histiocytes and all of the granules were seen within the cytoplasm. Quite a number of Langerhans cell granules were located near the Golgi apparatus. Several atypical granules very similar to the Langerhans cell granules could also be observed in these atypical histiocytes. These Langerhans cell granules were assumed to be directly derived from the Golgi apparatus and/or derived from the atypical granules which were secreted from the Golgi apparatus. The relationship between the Langerhans cell granules and the microtubules must also be considered, because Langerhans cell granules were found near the centrioles and microtubules.", "PMID": 961415}
{"id": "pubmed23n0032_4398", "title": "Accelerated serum sickness in the rabbit. III. Histopathological study of the development of tissue injuries of the heart.", "content": "Histiological analysis was performed on the lesions of the coronary artery and myocardium in accelerated serum sickness of the rabbit. Angiitis was seen to occur on the arterioles in the myocardium and was characterized by fibrinoid necrosis of the media with monocyte accumulation. Myocarditis was observed to be rather prominent in the right ventricle, where monocyte accumulation was associated with scattered degeneration or necrosis of myofibrils. In both lesions, the localization of antigen could hardly be detected in the inflammatory foci, suggesting the irrelevancy between the development of the tissue damages and the permanent deposition of immune complexes. Monocytes were seen to participate in the repair of the tissue injury.", "contents": "Accelerated serum sickness in the rabbit. III. Histopathological study of the development of tissue injuries of the heart. Histiological analysis was performed on the lesions of the coronary artery and myocardium in accelerated serum sickness of the rabbit. Angiitis was seen to occur on the arterioles in the myocardium and was characterized by fibrinoid necrosis of the media with monocyte accumulation. Myocarditis was observed to be rather prominent in the right ventricle, where monocyte accumulation was associated with scattered degeneration or necrosis of myofibrils. In both lesions, the localization of antigen could hardly be detected in the inflammatory foci, suggesting the irrelevancy between the development of the tissue damages and the permanent deposition of immune complexes. Monocytes were seen to participate in the repair of the tissue injury.", "PMID": 961416}
{"id": "pubmed23n0032_4399", "title": "An inhibitory effect of salivary gland extirpation on the induction of mammary tumors in rats by N,N'-2,7-fluorenylenebisacetamide.", "content": "N,N'2-2,7-Flourenylenebisacetamide (2.7-FAA) was administered orally to 34 female, strain AXC/I rats. Nineteen were pretreated with the extirpation of the salivary glands. The remaining 15 rats received no surgical treatment. Six of these 15 unoperated rats developed mammary carcinomas and seven developed tumors at other sites, whereas none of 19 operated rats had mammary tumors but 8 rats had tumors at other sites. No tumor developed in 4 operated control rats that were on normal diet. The results demonstrate that pretreatment with extirpation of the salivary glands inhibited mammary tumor induction in rats by 2,7-FAA although it did not inhibit induction of other type tumors. The mechanism by which induction of mammary tumors was inhibited is not clear. Ovarian atrophy and hepatic necrosis were observed in both operated and unoperated rats ingesting 2,7-FAA but did not seem to affect carcinogenesis by the carcinogen. Lower body weight was observed in operated rats but it is not considered to be the major cause of a complete inhibition of mammary tumor induction.", "contents": "An inhibitory effect of salivary gland extirpation on the induction of mammary tumors in rats by N,N'-2,7-fluorenylenebisacetamide. N,N'2-2,7-Flourenylenebisacetamide (2.7-FAA) was administered orally to 34 female, strain AXC/I rats. Nineteen were pretreated with the extirpation of the salivary glands. The remaining 15 rats received no surgical treatment. Six of these 15 unoperated rats developed mammary carcinomas and seven developed tumors at other sites, whereas none of 19 operated rats had mammary tumors but 8 rats had tumors at other sites. No tumor developed in 4 operated control rats that were on normal diet. The results demonstrate that pretreatment with extirpation of the salivary glands inhibited mammary tumor induction in rats by 2,7-FAA although it did not inhibit induction of other type tumors. The mechanism by which induction of mammary tumors was inhibited is not clear. Ovarian atrophy and hepatic necrosis were observed in both operated and unoperated rats ingesting 2,7-FAA but did not seem to affect carcinogenesis by the carcinogen. Lower body weight was observed in operated rats but it is not considered to be the major cause of a complete inhibition of mammary tumor induction.", "PMID": 961417}
{"id": "pubmed23n0032_4400", "title": "Pathological study on amyloidosis--the fine structure of amyloid-laden liver as revealed by freeze-etching.", "content": "Amyloid in freeze-etched specimen of liver of mice receiving subcutaneous in sodium caseinate was studied morphologically. The convex surface and cross surface of amyloid nodule were noted. The three dimentional structure of the amyloid fibrils which showed felt-like structure, globular profiles among amyloid fibrils and amyloid bundles were clearly visible. Amyloid bundles enveloped by the cytoplasmic membrane of the Kupffer cell projected from the deeply concave surface of the cytoplasmic membrane into the Disse's space and showed the appearance of ston-column-like structure. The amyloid bundles projecting from the invaginated cytoplasmic membrane were closely in contact with the cytoplasmic membrane and deeply rooted in the cytoplasm of the Kupffer cell.", "contents": "Pathological study on amyloidosis--the fine structure of amyloid-laden liver as revealed by freeze-etching. Amyloid in freeze-etched specimen of liver of mice receiving subcutaneous in sodium caseinate was studied morphologically. The convex surface and cross surface of amyloid nodule were noted. The three dimentional structure of the amyloid fibrils which showed felt-like structure, globular profiles among amyloid fibrils and amyloid bundles were clearly visible. Amyloid bundles enveloped by the cytoplasmic membrane of the Kupffer cell projected from the deeply concave surface of the cytoplasmic membrane into the Disse's space and showed the appearance of ston-column-like structure. The amyloid bundles projecting from the invaginated cytoplasmic membrane were closely in contact with the cytoplasmic membrane and deeply rooted in the cytoplasm of the Kupffer cell.", "PMID": 961418}
{"id": "pubmed23n0032_4401", "title": "Synaptic ribbon in the human pinealocyte.", "content": "An electron microscopic study of a pineal gland which had been obtained from a 3-year-old girl at autopsy revealed the presence of synaptic ribbon in it. Namely, in some parenchymal cells observed, synaptic ribbons, each of which was ca. 700 nm in length and with synaptic vesicles of up to 60 nm in diameter gathering on the surface, were found in the cytoplasmic area adjacent to the cell membrane. The substructure of the synaptic ribbon, i.e., parallel striae running inside along the longitudinal direction, was definitely observed. There is a hypothesis which has been almost accepted that the mammalian pinealocyte is homologous to the photoreceptor cell of the pineal organ in lower animals, and one of the morphological evidences which supports this speculation is the presence of sensory cell elements in both. Since, this kind of work has been hardly done with human materials, the above finding may offer a meaningful information which discloses a part of the real function of the pineal gland in man.", "contents": "Synaptic ribbon in the human pinealocyte. An electron microscopic study of a pineal gland which had been obtained from a 3-year-old girl at autopsy revealed the presence of synaptic ribbon in it. Namely, in some parenchymal cells observed, synaptic ribbons, each of which was ca. 700 nm in length and with synaptic vesicles of up to 60 nm in diameter gathering on the surface, were found in the cytoplasmic area adjacent to the cell membrane. The substructure of the synaptic ribbon, i.e., parallel striae running inside along the longitudinal direction, was definitely observed. There is a hypothesis which has been almost accepted that the mammalian pinealocyte is homologous to the photoreceptor cell of the pineal organ in lower animals, and one of the morphological evidences which supports this speculation is the presence of sensory cell elements in both. Since, this kind of work has been hardly done with human materials, the above finding may offer a meaningful information which discloses a part of the real function of the pineal gland in man.", "PMID": 961419}
{"id": "pubmed23n0032_4402", "title": "An autopsy case of liposarcoma with granulocytic leukemoid reaction.", "content": "A 57-year-old female with a large retroperitoneal tumor was atutopsied. She showed through the entire course of illness marked leukocytosis with the appearance of immature marrow cells, and this finding was hematologically interpreted as granulocytic leukemoid reaction. An intermittent pyrexia was also seen without infective etiology. The histologic diagnosis of the tumor was liposarcoma of the pleomorphic type. The tumor showed extensive necrosis, but no metastatic invasion was found. So-called leukemoid reaction appears sometimes together with malignant neoplasms. The majority of these tumors show widespread bone marrow metastases, and without bone marrow invasion they only rarely induce this reaction. Besides it is not common for leukemoid reaction to be evoked by the development of malignant mesenchymal tumors except for malignant lymphomas. The pathophysiologic process of the present of the present case is thought to be a rare occurrence.", "contents": "An autopsy case of liposarcoma with granulocytic leukemoid reaction. A 57-year-old female with a large retroperitoneal tumor was atutopsied. She showed through the entire course of illness marked leukocytosis with the appearance of immature marrow cells, and this finding was hematologically interpreted as granulocytic leukemoid reaction. An intermittent pyrexia was also seen without infective etiology. The histologic diagnosis of the tumor was liposarcoma of the pleomorphic type. The tumor showed extensive necrosis, but no metastatic invasion was found. So-called leukemoid reaction appears sometimes together with malignant neoplasms. The majority of these tumors show widespread bone marrow metastases, and without bone marrow invasion they only rarely induce this reaction. Besides it is not common for leukemoid reaction to be evoked by the development of malignant mesenchymal tumors except for malignant lymphomas. The pathophysiologic process of the present of the present case is thought to be a rare occurrence.", "PMID": 961420}
{"id": "pubmed23n0032_4403", "title": "Liposarcoma of the meninges. A case report.", "content": "A report on a liposarcoma in the meninges of a 70-year-old woman is presented. Pre-operative clinical and angiographic findings indicated that the tumour might be a meningeoma. Pathological examination revealed a predominantly lipoma-like, well-differentiated liposarcoma with round-cell and pleomorphic areas. The angiographic and pathological differential diagnosis are discussed.", "contents": "Liposarcoma of the meninges. A case report. A report on a liposarcoma in the meninges of a 70-year-old woman is presented. Pre-operative clinical and angiographic findings indicated that the tumour might be a meningeoma. Pathological examination revealed a predominantly lipoma-like, well-differentiated liposarcoma with round-cell and pleomorphic areas. The angiographic and pathological differential diagnosis are discussed.", "PMID": 961421}
{"id": "pubmed23n0032_4404", "title": "Thymic carcinoid. A case report.", "content": "A case of thymic carcinoid appearing in a 56-year-old man is presented. No endocrine disturbances were apparent. Mediastinal lymph node metastases were found at operation. The tumour was positive with Grimelius argyrophil silver stain. Ultrastructurally neurosecretory granules were seen.", "contents": "Thymic carcinoid. A case report. A case of thymic carcinoid appearing in a 56-year-old man is presented. No endocrine disturbances were apparent. Mediastinal lymph node metastases were found at operation. The tumour was positive with Grimelius argyrophil silver stain. Ultrastructurally neurosecretory granules were seen.", "PMID": 961422}
{"id": "pubmed23n0032_4405", "title": "Thymoma in a case of scleroderma.", "content": "A case of scleroderma with manifestations in the oesophagus and both lungs and a concomitant thymic lympho-epithelioma is presented. This case represents a further documentation of the well-established association between thymoma and collagen disorders.", "contents": "Thymoma in a case of scleroderma. A case of scleroderma with manifestations in the oesophagus and both lungs and a concomitant thymic lympho-epithelioma is presented. This case represents a further documentation of the well-established association between thymoma and collagen disorders.", "PMID": 961423}
{"id": "pubmed23n0032_4406", "title": "Carcinoid tumours. Frequency in a defined population during a 12-year period.", "content": "The frequency of carcinoids was studied in a 12-year-period (1958-1969) in Malm\u00f6, a town with a population of 220,000 at the beginning of the period and 250,000 at the end of it. Of all persons who had died in Malm\u00f6, 46 per cent were necropsied in the first year of the study and 70 per cent in the last (altogether 62.6 per cent). The series was examined in a uniform way at one department of pathology. Carcinoid tumours were found in 1.22 per cent (199 patients) of patients comprised in the entire necropsy series (16,294 autopsies). Bronchial carcinoid accounted for 0.1 percent, the remaining lesions were found in the digestive tract. About 90 per cent of the carcinoids were found incidentally at necropsy. During the same period, 44 carcinoids were diagnosed in surgical specimens examined in Malm\u00f6. The average annual frequency of carcinoid in the entire series was about 8.4 per 100,000 inhabitants, which is about 7 times as high as that recorded in the National Cancer Register applying to the whole of Sweden. The value of such country-wide reports is discussed. The carcinoid syndrome is extremely rare and was observed only once during the entire 12-year-period. The localization, frequency of metastases and sex-distribution of carcinoids are described and discussed in detail.", "contents": "Carcinoid tumours. Frequency in a defined population during a 12-year period. The frequency of carcinoids was studied in a 12-year-period (1958-1969) in Malm\u00f6, a town with a population of 220,000 at the beginning of the period and 250,000 at the end of it. Of all persons who had died in Malm\u00f6, 46 per cent were necropsied in the first year of the study and 70 per cent in the last (altogether 62.6 per cent). The series was examined in a uniform way at one department of pathology. Carcinoid tumours were found in 1.22 per cent (199 patients) of patients comprised in the entire necropsy series (16,294 autopsies). Bronchial carcinoid accounted for 0.1 percent, the remaining lesions were found in the digestive tract. About 90 per cent of the carcinoids were found incidentally at necropsy. During the same period, 44 carcinoids were diagnosed in surgical specimens examined in Malm\u00f6. The average annual frequency of carcinoid in the entire series was about 8.4 per 100,000 inhabitants, which is about 7 times as high as that recorded in the National Cancer Register applying to the whole of Sweden. The value of such country-wide reports is discussed. The carcinoid syndrome is extremely rare and was observed only once during the entire 12-year-period. The localization, frequency of metastases and sex-distribution of carcinoids are described and discussed in detail.", "PMID": 961424}
{"id": "pubmed23n0032_4407", "title": "Thymoma metastasizing to extrathoracic sites. A case report.", "content": "In the case presented, metastases to extrathoracic sites developed in a 53-year-old man four years after the diagnosis of inoperable thymoma had been established. The appearance of metastases was preced by an extensive intrathoracic invasion of the tumour. The thymoma was of epithelial subtype which was the most common (67 percent) type also in the previously published 29 cases of thymomas metastasizing to extrathoracic sites.", "contents": "Thymoma metastasizing to extrathoracic sites. A case report. In the case presented, metastases to extrathoracic sites developed in a 53-year-old man four years after the diagnosis of inoperable thymoma had been established. The appearance of metastases was preced by an extensive intrathoracic invasion of the tumour. The thymoma was of epithelial subtype which was the most common (67 percent) type also in the previously published 29 cases of thymomas metastasizing to extrathoracic sites.", "PMID": 961425}
{"id": "pubmed23n0032_4408", "title": "Heteroteansplantation of a human mammary carcinoma to the mouse mutant nude.", "content": "A human mammary carcinoma was heterotransplanted to athymic nude mice. Viable tumour tissue was maintained throughout the observation periods which, because of the limited life span of nude mice, were relatively short; in one animal surviving for a longer period, actual tumour growth was observed. It is concluded that human mammary carcinoma is capable of growing in nude mice but, because of the frequently low growth rate of these tumours, prolonged periods of observation are required in order to reveal such growth.", "contents": "Heteroteansplantation of a human mammary carcinoma to the mouse mutant nude. A human mammary carcinoma was heterotransplanted to athymic nude mice. Viable tumour tissue was maintained throughout the observation periods which, because of the limited life span of nude mice, were relatively short; in one animal surviving for a longer period, actual tumour growth was observed. It is concluded that human mammary carcinoma is capable of growing in nude mice but, because of the frequently low growth rate of these tumours, prolonged periods of observation are required in order to reveal such growth.", "PMID": 961426}
{"id": "pubmed23n0032_4409", "title": "Urothelial hyperplasia of the renal papillae in female Sprague-Dawley rats induced by long term feeding of phenacetin.", "content": "Twenty-one of 40 rats fed phenacetin in the diet for up to 86 weeks developed urothelial hyperplasia of the renal papillae. Two of 30 rats in the control group had similar changes associated with chronic pyelitis. The difference is statistically significant (p less than 0.01).", "contents": "Urothelial hyperplasia of the renal papillae in female Sprague-Dawley rats induced by long term feeding of phenacetin. Twenty-one of 40 rats fed phenacetin in the diet for up to 86 weeks developed urothelial hyperplasia of the renal papillae. Two of 30 rats in the control group had similar changes associated with chronic pyelitis. The difference is statistically significant (p less than 0.01).", "PMID": 961427}
{"id": "pubmed23n0032_4410", "title": "Repair in arterial tissue. Electron microscopy of Evans blue vital stained embolectomy catheter lesion of the rabbit thoracic aorta.", "content": "The rabbit thoracic aorta was studied by EM and vital staining with Evans blue at vaired points of time after a single lesion produced by an embolectomy catheter. EM of white areas: the surface cells of the myo-intimal thickening resembled endothelial cells with a discrete occurrence of microfilaments inside the plasma-membranes and rather differentiated flap-like junctions. EM of blue areas: the surface cells resembled modified smooth muscle cells with a heavily contrasted zone just inside the luminal plasma membrane containing closely packed microfilaments. The cell contacts either were missing, or they presented undifferentiated side-to-side contacts.", "contents": "Repair in arterial tissue. Electron microscopy of Evans blue vital stained embolectomy catheter lesion of the rabbit thoracic aorta. The rabbit thoracic aorta was studied by EM and vital staining with Evans blue at vaired points of time after a single lesion produced by an embolectomy catheter. EM of white areas: the surface cells of the myo-intimal thickening resembled endothelial cells with a discrete occurrence of microfilaments inside the plasma-membranes and rather differentiated flap-like junctions. EM of blue areas: the surface cells resembled modified smooth muscle cells with a heavily contrasted zone just inside the luminal plasma membrane containing closely packed microfilaments. The cell contacts either were missing, or they presented undifferentiated side-to-side contacts.", "PMID": 961428}
{"id": "pubmed23n0032_4411", "title": "Prolonged survival of AKR mice treated with the saponin adjuvant Quil A.", "content": "Treatment of AKR mice with the saponin Quil A delayed their death in spontaneous leukaemia. In vitro tests did not demonstrate any influence on infection of rat cells with Kirsten sarcoma virus. Quil A in the concentration nanogram/ml doubled the mitogen response of AKR spleen cells to lipopolysaccharide.", "contents": "Prolonged survival of AKR mice treated with the saponin adjuvant Quil A. Treatment of AKR mice with the saponin Quil A delayed their death in spontaneous leukaemia. In vitro tests did not demonstrate any influence on infection of rat cells with Kirsten sarcoma virus. Quil A in the concentration nanogram/ml doubled the mitogen response of AKR spleen cells to lipopolysaccharide.", "PMID": 961429}
{"id": "pubmed23n0032_4412", "title": "Cellular monosaccharide patterns of Neisseriaceae.", "content": "Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in \"true neisseriae\" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic \"monosaccharide\" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.", "contents": "Cellular monosaccharide patterns of Neisseriaceae. Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in \"true neisseriae\" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic \"monosaccharide\" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.", "PMID": 961430}
{"id": "pubmed23n0032_4413", "title": "The effect of endo-enteric development of Toxoplasma gondii on the ultrastructure of epithelial cells of the small intestine of infected cats.", "content": "Toxoplasma gondii undergoing endo-enteric development in the small intestine of the cat affects the ultrastructure of the epithelial cells. The epithelial cells from infected cats have swollen rough endoplasmic reticulum and the mitochondria may be swollen with degenerate cristae. A statistically significant shortening of the microvilli of the epithelial cells was demonstrated. It appeared that the effect on the microvilli length was related to the parasite distribution within the small intestine. These changes occur in both cells with and without parasites present at the level of the sections studied. The abnormalities are quickly rectified after the disappearance of the endo-enteric forms of Toxoplasma.", "contents": "The effect of endo-enteric development of Toxoplasma gondii on the ultrastructure of epithelial cells of the small intestine of infected cats. Toxoplasma gondii undergoing endo-enteric development in the small intestine of the cat affects the ultrastructure of the epithelial cells. The epithelial cells from infected cats have swollen rough endoplasmic reticulum and the mitochondria may be swollen with degenerate cristae. A statistically significant shortening of the microvilli of the epithelial cells was demonstrated. It appeared that the effect on the microvilli length was related to the parasite distribution within the small intestine. These changes occur in both cells with and without parasites present at the level of the sections studied. The abnormalities are quickly rectified after the disappearance of the endo-enteric forms of Toxoplasma.", "PMID": 961431}
{"id": "pubmed23n0032_4414", "title": "Localization of urease activity in Ureaplasma urealyticum cells.", "content": "Measurements of urease activity of various cell fractions of U. urealyticum showed that this activity was confined to the soluble fraction of the cytoplasm. It was attempted to devise a method for electron microscopic detection of the sites of urease activity based on precipitation of electron dense MnO2 at the alkaline pH created by hydrolysis of urea. The results obtained by this method supported the previous results indicating a cytoplasmatic localization of the urease activity in the cells. Helical ribosome patterns were observed when glutaraldehyde fixed cells were treated with the cytochemical test solutions.", "contents": "Localization of urease activity in Ureaplasma urealyticum cells. Measurements of urease activity of various cell fractions of U. urealyticum showed that this activity was confined to the soluble fraction of the cytoplasm. It was attempted to devise a method for electron microscopic detection of the sites of urease activity based on precipitation of electron dense MnO2 at the alkaline pH created by hydrolysis of urea. The results obtained by this method supported the previous results indicating a cytoplasmatic localization of the urease activity in the cells. Helical ribosome patterns were observed when glutaraldehyde fixed cells were treated with the cytochemical test solutions.", "PMID": 961432}
{"id": "pubmed23n0032_4415", "title": "Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy.", "content": "In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.", "contents": "Immunoelectroosmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy. In two separate studies, faecal samples were collected from children with acute gastroenteritis; the samples were tested both by electron microscopy and immunoelectroosmophoresis with a view to detect reo-like virus. In 94 per cent and 100 per cent of samples positive by electron microscopy in the first and second material, respectively, a precipitin line was found after electrophoresis, using a guinea pig antiserum against a purified bovine virus. No false positives were detected by this method.", "PMID": 961433}
{"id": "pubmed23n0032_4416", "title": "Purification of the O antigen of Bacteroides fragilis ss. fragilis NCTC 9343 from phenol-water extracts by gel filtration and chromatography on deae-cellulose and hydroxylapatite.", "content": "O antigen extracted from whole cells of Bacteroides fragilis ss. fragilis NCTC 9343 with 45 per cent aqueous phenol has been purified by gel filtration and chromatography. First, the water phase was treated with RNase and DNase and passed through a column of agarose. The chromatographic procedures included ion exchange on a column of DEAE-cellulose and adsorption to hydroxylapatite. The O antigen was eluted from the DEAE-cellulose with a gradient of NaCl, and from the column of hydroxylapatite with 1 M phosphate buffer, pH 6.8. Inhibition of indirect haemagglutination was used to detect the O antigen in the eluates.", "contents": "Purification of the O antigen of Bacteroides fragilis ss. fragilis NCTC 9343 from phenol-water extracts by gel filtration and chromatography on deae-cellulose and hydroxylapatite. O antigen extracted from whole cells of Bacteroides fragilis ss. fragilis NCTC 9343 with 45 per cent aqueous phenol has been purified by gel filtration and chromatography. First, the water phase was treated with RNase and DNase and passed through a column of agarose. The chromatographic procedures included ion exchange on a column of DEAE-cellulose and adsorption to hydroxylapatite. The O antigen was eluted from the DEAE-cellulose with a gradient of NaCl, and from the column of hydroxylapatite with 1 M phosphate buffer, pH 6.8. Inhibition of indirect haemagglutination was used to detect the O antigen in the eluates.", "PMID": 961434}
{"id": "pubmed23n0032_4417", "title": "NDV-O agglutinins in Mycoplasma pneumoniae infections: association with pneumonia.", "content": "Agglutinins against human O erythrocytes modified by the B1 strain of Newcastle disease virus were studied in paired sera of 148 patients with a 4-fold or greater rise in complement-fixing M. pneumoniae antibodies. The proportion of cases with a significant rise in NDV-O agglutinins was higher agmong the patients with pneumonia than among those with neurological or other clinical manifestations.", "contents": "NDV-O agglutinins in Mycoplasma pneumoniae infections: association with pneumonia. Agglutinins against human O erythrocytes modified by the B1 strain of Newcastle disease virus were studied in paired sera of 148 patients with a 4-fold or greater rise in complement-fixing M. pneumoniae antibodies. The proportion of cases with a significant rise in NDV-O agglutinins was higher agmong the patients with pneumonia than among those with neurological or other clinical manifestations.", "PMID": 961435}
{"id": "pubmed23n0032_4418", "title": "Detection and differentiation of immune complexes and IgG aggregates by a complement consumption assay.", "content": "The applicability of a complement consumption assay as a means by which to detect IgG aggregates and immune complexes in serum was examined. Both heavy (greater than or equal to 19S) and intermediate (11-17S) IgG aggregates were detected and the sensitivity of the assay was greater than or equal to 10 mug aggregated IgG/ml. BSA anti-BSA complexes, formed in slight antibody excess, were detected at a BSA concentration of 200 ng/ml. NHS stored at 4degreesC for greater than or equal to 2-3 weeks or at -20degreesC for more than 3 months developed distinct anticomplementarity (AC). This background AC, due to IgG aggregate formation, was reduced by heating the serum at 56degreesC for 50 min prior to testing. A similar reduction of AC and C1q fixation was observed when IgG aggregated at 61degreesC or 63degreesC was heated further at 56degreesC for 50 min. The abatement of AC could not be correlated to a change in IgG aggregation size. In contrast, AC of preformed antigen-antibody complexes was not reduced by this heat treatment.", "contents": "Detection and differentiation of immune complexes and IgG aggregates by a complement consumption assay. The applicability of a complement consumption assay as a means by which to detect IgG aggregates and immune complexes in serum was examined. Both heavy (greater than or equal to 19S) and intermediate (11-17S) IgG aggregates were detected and the sensitivity of the assay was greater than or equal to 10 mug aggregated IgG/ml. BSA anti-BSA complexes, formed in slight antibody excess, were detected at a BSA concentration of 200 ng/ml. NHS stored at 4degreesC for greater than or equal to 2-3 weeks or at -20degreesC for more than 3 months developed distinct anticomplementarity (AC). This background AC, due to IgG aggregate formation, was reduced by heating the serum at 56degreesC for 50 min prior to testing. A similar reduction of AC and C1q fixation was observed when IgG aggregated at 61degreesC or 63degreesC was heated further at 56degreesC for 50 min. The abatement of AC could not be correlated to a change in IgG aggregation size. In contrast, AC of preformed antigen-antibody complexes was not reduced by this heat treatment.", "PMID": 961436}
{"id": "pubmed23n0032_4419", "title": "Transplantation of human adipose tissue to nude mice.", "content": "Human adipose tissue was transplanted to the mouse mutant nude (nu/nu). All the grafts were accepted and contained fat cells easily distinguishable from those of the mouse. No detectable relation between the histological pictures before and after grafting was found. In some transplants  nerve tissue, and in others macrophages containing fat droplets, were found. The fat tissue graft might be useful for investigation of the influence of various hormones on human fat cells.", "contents": "Transplantation of human adipose tissue to nude mice. Human adipose tissue was transplanted to the mouse mutant nude (nu/nu). All the grafts were accepted and contained fat cells easily distinguishable from those of the mouse. No detectable relation between the histological pictures before and after grafting was found. In some transplants  nerve tissue, and in others macrophages containing fat droplets, were found. The fat tissue graft might be useful for investigation of the influence of various hormones on human fat cells.", "PMID": 961437}
{"id": "pubmed23n0032_4420", "title": "An examination of the \"star-phenomenon\", a three component immunoprecipitation involving staphylococcal protein A.", "content": "The co-precipitation called \"star-phenomenon\" occurred between the three component system: protein A, an IgG forming soluble complexes with protein A and F (ab')2-preparations of human IgG, guinea pig IgG or rabbit anti-staphylococcal IgG. Co-precipitation also occurred if the IgG was replaced by normal human Fc fragments. On a protein A column the human F (ab')2-preparation was separated into a major non-reactive and a minor reactive fraction. Only the latter contained Fc-structures, these being isolated on an anti-Fc column and found to belong to undigested IgG. The \"star\"-forming protein A reactive F (ab')2-fragments were washed through the anti-Fc column. The F (ab')2 fraction from rabbit anti-staphylococcal IgG contained no Fc-structures and only a small portion containing anti-protein A activity was active in co-precipitation.", "contents": "An examination of the \"star-phenomenon\", a three component immunoprecipitation involving staphylococcal protein A. The co-precipitation called \"star-phenomenon\" occurred between the three component system: protein A, an IgG forming soluble complexes with protein A and F (ab')2-preparations of human IgG, guinea pig IgG or rabbit anti-staphylococcal IgG. Co-precipitation also occurred if the IgG was replaced by normal human Fc fragments. On a protein A column the human F (ab')2-preparation was separated into a major non-reactive and a minor reactive fraction. Only the latter contained Fc-structures, these being isolated on an anti-Fc column and found to belong to undigested IgG. The \"star\"-forming protein A reactive F (ab')2-fragments were washed through the anti-Fc column. The F (ab')2 fraction from rabbit anti-staphylococcal IgG contained no Fc-structures and only a small portion containing anti-protein A activity was active in co-precipitation.", "PMID": 961438}
{"id": "pubmed23n0032_4421", "title": "Sources of carbon skeleton of alanine released from skeletal muscle.", "content": "The formation of alanine, glutamate, and aspartate from muscle was studied in the isolated perfused hindquarter of rats fasted for 48 h. Tracer doses of (14C) compounds with high specific activity were tested as precursors for the amino acids. Total amounts and radioactivities of the tested amino acids were determined. Alanine was produced more efficiently than glutamate and aspartate even if no exogenous substrate was offered. (14C) Pyruvate was most efficient as precursor of labeled alanine. However, labeled leucine, propionic acid, valine and fumaric acid also produced labeled alanine efficiently. The efficiency as precursor for labeled alanine seemed to be related to the ability to label intermediates in the citric acid cycle in the perfused muscle. From the relation between the ability to label alanine in the perfusion medium and lactate and succinate in the muscle it is suggested that pyruvate may be produced intramitochondrially and is efficiently transaminated to alanine in this compartment.", "contents": "Sources of carbon skeleton of alanine released from skeletal muscle. The formation of alanine, glutamate, and aspartate from muscle was studied in the isolated perfused hindquarter of rats fasted for 48 h. Tracer doses of (14C) compounds with high specific activity were tested as precursors for the amino acids. Total amounts and radioactivities of the tested amino acids were determined. Alanine was produced more efficiently than glutamate and aspartate even if no exogenous substrate was offered. (14C) Pyruvate was most efficient as precursor of labeled alanine. However, labeled leucine, propionic acid, valine and fumaric acid also produced labeled alanine efficiently. The efficiency as precursor for labeled alanine seemed to be related to the ability to label intermediates in the citric acid cycle in the perfused muscle. From the relation between the ability to label alanine in the perfusion medium and lactate and succinate in the muscle it is suggested that pyruvate may be produced intramitochondrially and is efficiently transaminated to alanine in this compartment.", "PMID": 961439}
{"id": "pubmed23n0032_4422", "title": "Autonomic nerve control of the swimbladder of the goldsinny wrasse, Ctenolabrus rupestris.", "content": "The autonomic nerve control of the swimbladder of the goldsinny wrasse has been studied by experiments with isolated strips from the muscularis mucosae, drug effects on gas secretion in vivo, fluorescent histochemistry and quantitative analysis of catecholamine content. Vagotomy, atropine and mecamylamine significantly inhibited induced gas secretion, suggesting a cholinergic vagal secretory innervation of the gas gland. The anterior part of the mucosa (secretory part) is contracted by acetylcholine and alpha-adrenoceptor agonists. These responses are competitively antagonized by atropine and phentolamine or yohimbine respectively. The posterior (resorbent) part of the mucosa is contracted by acetylcholine, but beta-adrenoceptor agonists relax the acetylcholine-precontracted preparations. These responses are competitively blocked by atropine and propranolol respectively. The pA2-values from all experiments with antagonists are well comparable with values obtained for the same drugs in other teleost or mammalian tissues. Tyramine, 5-hydroxytryptamine or phenylephrine had mixed effects on the posterior part. This effect is at least in part due to release of nervously stored catecholamines. Fluorescent histochemistry revealed a strong innervation by both smooth and varicose adrenergic fibres in all parts of the swimbladder, noradrenaline being the dominant catecholamine. Yellow fluorescent cells, which may contain 5-hydroxytryptamine, were also seen in the swimbladder mucosa. No conclusive evidence for a cholinergic innervation of the muscularis mucosae was obtained.", "contents": "Autonomic nerve control of the swimbladder of the goldsinny wrasse, Ctenolabrus rupestris. The autonomic nerve control of the swimbladder of the goldsinny wrasse has been studied by experiments with isolated strips from the muscularis mucosae, drug effects on gas secretion in vivo, fluorescent histochemistry and quantitative analysis of catecholamine content. Vagotomy, atropine and mecamylamine significantly inhibited induced gas secretion, suggesting a cholinergic vagal secretory innervation of the gas gland. The anterior part of the mucosa (secretory part) is contracted by acetylcholine and alpha-adrenoceptor agonists. These responses are competitively antagonized by atropine and phentolamine or yohimbine respectively. The posterior (resorbent) part of the mucosa is contracted by acetylcholine, but beta-adrenoceptor agonists relax the acetylcholine-precontracted preparations. These responses are competitively blocked by atropine and propranolol respectively. The pA2-values from all experiments with antagonists are well comparable with values obtained for the same drugs in other teleost or mammalian tissues. Tyramine, 5-hydroxytryptamine or phenylephrine had mixed effects on the posterior part. This effect is at least in part due to release of nervously stored catecholamines. Fluorescent histochemistry revealed a strong innervation by both smooth and varicose adrenergic fibres in all parts of the swimbladder, noradrenaline being the dominant catecholamine. Yellow fluorescent cells, which may contain 5-hydroxytryptamine, were also seen in the swimbladder mucosa. No conclusive evidence for a cholinergic innervation of the muscularis mucosae was obtained.", "PMID": 961440}
{"id": "pubmed23n0032_4423", "title": "The effect of nicotine on intra-axonal transport in cholinergic motor neurons of the rat; influence of acutely administered, non-toxic doses.", "content": "The intra-axonal transport of acetylcholine (ACh) and ACh-metabolizing enzymes in rat sciatic nerves was studied following nicotine treatment. The nicotine was administered in a low dose (30 mug/kg) s.c. every second h for 18-22 h, to mimic the serum-concentrations of a cigarette smoker. Control rats received s.c. injections of the vehicle. The main effects were observed regarding the ACh-esterase (AChE) activity. In unoperated nerves the level was decreased by about 10%. In 12 h crushed nerves the accumulation of enzyme activity proximal to the crush was markedly reduced in the nicotine-treated group, indicating a reduction in amount of proximo-distally transported enzyme activity. The ACh-levels were unchanged except at 12 h postoperatively when a small increase proximal to the crush was observed. The choline-acetyl transferase (CAT) activity in unoperated nerves was similar in both groups of rats; the 7 h accumulation above the crush, however, was somewhat reduced after nicotine treatment. The possible causes of the observed nicotine induced changes are so far not known.", "contents": "The effect of nicotine on intra-axonal transport in cholinergic motor neurons of the rat; influence of acutely administered, non-toxic doses. The intra-axonal transport of acetylcholine (ACh) and ACh-metabolizing enzymes in rat sciatic nerves was studied following nicotine treatment. The nicotine was administered in a low dose (30 mug/kg) s.c. every second h for 18-22 h, to mimic the serum-concentrations of a cigarette smoker. Control rats received s.c. injections of the vehicle. The main effects were observed regarding the ACh-esterase (AChE) activity. In unoperated nerves the level was decreased by about 10%. In 12 h crushed nerves the accumulation of enzyme activity proximal to the crush was markedly reduced in the nicotine-treated group, indicating a reduction in amount of proximo-distally transported enzyme activity. The ACh-levels were unchanged except at 12 h postoperatively when a small increase proximal to the crush was observed. The choline-acetyl transferase (CAT) activity in unoperated nerves was similar in both groups of rats; the 7 h accumulation above the crush, however, was somewhat reduced after nicotine treatment. The possible causes of the observed nicotine induced changes are so far not known.", "PMID": 961441}
{"id": "pubmed23n0032_4424", "title": "The influence of temperature on the time course of the mechanical activity in rabbit papillary muscle.", "content": "The influence of temperature on the time course of the mechanical activity was investigated in isolated papillary muscles of the rabbit. By using the \"damped release\" technique activity curves were determined in terms of velocity of shortening of the contractile unit at a constant length. At any of the temperatures studied, the time course of the mechanical activity exhibited a slow onset and did not form a distinct plateau. At low contraction frequencies and normal (2 mM) calcium concentration lowering the temperature from 32.5 to 26.5 degrees C did not markedly affect the rate of development of the activity but increased the peak amplitude and the total duration of the activity as well as the time from the stimulus to 90% of the maximum. It also resulted in a slower decay phase. At higher contraction frequencies and/or increased calcium concentration the rate of development of the activity was reduced by decreasing the temperature from 32.5 degrees C to 26.5 degrees C, whereas the maximal activity reached similar levels at the two temperatures. It is concluded that an alteration in temperature affects several different steps in myocardial excitation-contraction coupling, the net change in maximum amplitude of the mechanical activity being influenced by both extracellular calcium concentration and contraction frequency.", "contents": "The influence of temperature on the time course of the mechanical activity in rabbit papillary muscle. The influence of temperature on the time course of the mechanical activity was investigated in isolated papillary muscles of the rabbit. By using the \"damped release\" technique activity curves were determined in terms of velocity of shortening of the contractile unit at a constant length. At any of the temperatures studied, the time course of the mechanical activity exhibited a slow onset and did not form a distinct plateau. At low contraction frequencies and normal (2 mM) calcium concentration lowering the temperature from 32.5 to 26.5 degrees C did not markedly affect the rate of development of the activity but increased the peak amplitude and the total duration of the activity as well as the time from the stimulus to 90% of the maximum. It also resulted in a slower decay phase. At higher contraction frequencies and/or increased calcium concentration the rate of development of the activity was reduced by decreasing the temperature from 32.5 degrees C to 26.5 degrees C, whereas the maximal activity reached similar levels at the two temperatures. It is concluded that an alteration in temperature affects several different steps in myocardial excitation-contraction coupling, the net change in maximum amplitude of the mechanical activity being influenced by both extracellular calcium concentration and contraction frequency.", "PMID": 961442}
{"id": "pubmed23n0032_4425", "title": "Influence of acidosis on noradrenaline-induced vasoconstriction in adipose tissue and skeletal muscle.", "content": "Vasoconstriction due to parallel i.a. injections of NA were studied in subcutaneous adipose tissue and gracilis muscle preparations in dogs. The vasoconstrictor response to NA was significantly lower in adipose tissue than in muscle. Only in muscle did acidosis inhibit NA-induced vasoconstriction. The beta-receptor antagonist propranolol increased the vasoconstrictor response in adipose tissue to the level of skeletal muscle. The lack of significant inhibition of NA-induced vasoconstriction in adipose tissue may be due to the simultaneous inhibition of two opposing mechanisms-alpha-adrenergic vasoconstriction and beta-adrenergic vasodilatation. After propranolol acidosis inhibited NA-induced vasoconstriction equally in adipose tissue and muscle. The difference between adipose tissue and muscle may thus be due to a greater importance of a beta-adrenergic vasodilator mechanism in the former tissue. The metabolic response to isoprenaline was inhibited by acidosis, while the direct vasodilatation was unaffected. It is suggested that the beta-adrenergic vasodilator mechanism that is inhibited by acidosis is related to the metabolism of the tissue.", "contents": "Influence of acidosis on noradrenaline-induced vasoconstriction in adipose tissue and skeletal muscle. Vasoconstriction due to parallel i.a. injections of NA were studied in subcutaneous adipose tissue and gracilis muscle preparations in dogs. The vasoconstrictor response to NA was significantly lower in adipose tissue than in muscle. Only in muscle did acidosis inhibit NA-induced vasoconstriction. The beta-receptor antagonist propranolol increased the vasoconstrictor response in adipose tissue to the level of skeletal muscle. The lack of significant inhibition of NA-induced vasoconstriction in adipose tissue may be due to the simultaneous inhibition of two opposing mechanisms-alpha-adrenergic vasoconstriction and beta-adrenergic vasodilatation. After propranolol acidosis inhibited NA-induced vasoconstriction equally in adipose tissue and muscle. The difference between adipose tissue and muscle may thus be due to a greater importance of a beta-adrenergic vasodilator mechanism in the former tissue. The metabolic response to isoprenaline was inhibited by acidosis, while the direct vasodilatation was unaffected. It is suggested that the beta-adrenergic vasodilator mechanism that is inhibited by acidosis is related to the metabolism of the tissue.", "PMID": 961443}
{"id": "pubmed23n0032_4426", "title": "Exteroceptive influences on the lumbar back muscle tone and reflexes in the cat.", "content": "The exteroceptive influences on tonic activity and on stretch reflexes of the longissimus dorsi and multifidus spinae muscles were investiagted in decerebrate, spinal and chloralose-anesthetized cats. Adequate skin stimulation was used to map out facilitatory and inhibitory skin areas on the trunk and the extremities. On the trunk facilitatory areas are relatively large and located at the dorsal side while inhibitory areas are confined to the ventrolateral part of the contralateral body half. The facilitatory skin fields are of approximately the same size in decerebrate and spinal cats. On leg skin stimulation facilitation can be evoked from the ipsilateral hind limb while inhibition results from stimulation of the other limbs. Spinal cord transection increased excitatory effects of ipsilateral hind limb stimulation. Reflex responses in the back muscles to applied stretch are described. These reflexes were used as test reflexes in experiments with conditioning stimulation of the peripheral nerves supplying skin areas from which effects on back muscle activity were evoked by adequate stimulation. The conditioning-test experiments and those using adequate stimulation show that the longissimus dorsi and multifidus spinae are activated or facilitated by an ipsilateral stimulus to skin afferents. The extent of the effects induced by stimulation of skin differs in the types of preparation used. These differences may be accounted for by assuming a supraspinal control of the reflex pathways studied.", "contents": "Exteroceptive influences on the lumbar back muscle tone and reflexes in the cat. The exteroceptive influences on tonic activity and on stretch reflexes of the longissimus dorsi and multifidus spinae muscles were investiagted in decerebrate, spinal and chloralose-anesthetized cats. Adequate skin stimulation was used to map out facilitatory and inhibitory skin areas on the trunk and the extremities. On the trunk facilitatory areas are relatively large and located at the dorsal side while inhibitory areas are confined to the ventrolateral part of the contralateral body half. The facilitatory skin fields are of approximately the same size in decerebrate and spinal cats. On leg skin stimulation facilitation can be evoked from the ipsilateral hind limb while inhibition results from stimulation of the other limbs. Spinal cord transection increased excitatory effects of ipsilateral hind limb stimulation. Reflex responses in the back muscles to applied stretch are described. These reflexes were used as test reflexes in experiments with conditioning stimulation of the peripheral nerves supplying skin areas from which effects on back muscle activity were evoked by adequate stimulation. The conditioning-test experiments and those using adequate stimulation show that the longissimus dorsi and multifidus spinae are activated or facilitated by an ipsilateral stimulus to skin afferents. The extent of the effects induced by stimulation of skin differs in the types of preparation used. These differences may be accounted for by assuming a supraspinal control of the reflex pathways studied.", "PMID": 961444}
{"id": "pubmed23n0032_4427", "title": "Secretory and motor effects in the submaxillary gland of the rat on intraarterial administration of some polypeptides and autonomic drugs.", "content": "Bradykinin, oxytocin, physalaemin and some autonomic drugs were injected in to the common carotid artery. Physalaemin evoked secretion and a pressure rise in the submaxillary duct. A duct pressure rise could be elicited by bradykinin which did not evoke secretion. Autonomic blocking agents did not diminish secretion evoked by physalaemin and did not change pressure responses elicited by bradykinin or physalaemin. Neither secretion, nor duct pressure changes could be recorded after administration of oxytocin. In agreement with previous experiments secretion evoked by autonomic drugs was found to be mediated via cholinergic, alpha- and beta-adrenergic receptors, while motor effects were due to activation of cholinergic and alpha-adrenergic receptors.", "contents": "Secretory and motor effects in the submaxillary gland of the rat on intraarterial administration of some polypeptides and autonomic drugs. Bradykinin, oxytocin, physalaemin and some autonomic drugs were injected in to the common carotid artery. Physalaemin evoked secretion and a pressure rise in the submaxillary duct. A duct pressure rise could be elicited by bradykinin which did not evoke secretion. Autonomic blocking agents did not diminish secretion evoked by physalaemin and did not change pressure responses elicited by bradykinin or physalaemin. Neither secretion, nor duct pressure changes could be recorded after administration of oxytocin. In agreement with previous experiments secretion evoked by autonomic drugs was found to be mediated via cholinergic, alpha- and beta-adrenergic receptors, while motor effects were due to activation of cholinergic and alpha-adrenergic receptors.", "PMID": 961445}
{"id": "pubmed23n0032_4428", "title": "Gastric clearance of gastrin during acid secretory responses to vagal activation and its effect on peripheral gastrin levels. A study on anesthetized cats.", "content": "Gastrin release and gastric acid secretion were induced by electrical stimulation of the vagi in anesthetized cats. No correlation was found between the peripheral gastrin levels and the magnitudes of the concomitant secretory responses. In experiments in which a high rate of secretion was induced the peripheral gastrin levels were found to be unchanged or at most slightly elevated, whereas the levels were sometimes substantially elevated in animals in which there was little or no acid secretory response. An arterio-venous difference in gastrin levels was detected in the corpus blood during periods of gastric secretion. This clearance of gastrin (104-540 pg/min) was large enough to influence the peripheral gastrin levels. The mechanism behind and the significance of the gastric clearance of gastrin is discussed.", "contents": "Gastric clearance of gastrin during acid secretory responses to vagal activation and its effect on peripheral gastrin levels. A study on anesthetized cats. Gastrin release and gastric acid secretion were induced by electrical stimulation of the vagi in anesthetized cats. No correlation was found between the peripheral gastrin levels and the magnitudes of the concomitant secretory responses. In experiments in which a high rate of secretion was induced the peripheral gastrin levels were found to be unchanged or at most slightly elevated, whereas the levels were sometimes substantially elevated in animals in which there was little or no acid secretory response. An arterio-venous difference in gastrin levels was detected in the corpus blood during periods of gastric secretion. This clearance of gastrin (104-540 pg/min) was large enough to influence the peripheral gastrin levels. The mechanism behind and the significance of the gastric clearance of gastrin is discussed.", "PMID": 961446}
{"id": "pubmed23n0032_4429", "title": "Liver and heart mitochondrial succinate dehydrogenase activity of newborn rats in anoxic hypoxia and starvation.", "content": "Succinate dehydrogenase activity was determined in the liver and heart of newborn rats after 3 and 48 hours' exposure to anoxic hypoxia (10% O2) and after 48 hours' starvation. Control determinations were made on newborn animals of corresponding ages, full term foetuses (21 days), infantile (1 and 2 weeks) and full grown animals. Hypoxia for 3 h had no influence on succinate dehydrogenase activity at all in either the heart or liver mitochondria of the newborn animals. After 48 h no difference was observed in the liver between the hypoxic animals and the starved controls of the same age, though starvation itself had resulted in a significant increase in activity, as much as 42%. When liver mitochondrial succinate dehydrogenase in normal mitochondria was activated by preincubation mitochondria with the substrate, the activity increase obtained was greater than that resulting from starvation. The increase in activity in the heart of the hypoxic or starved animals was not significant (less than 10%).", "contents": "Liver and heart mitochondrial succinate dehydrogenase activity of newborn rats in anoxic hypoxia and starvation. Succinate dehydrogenase activity was determined in the liver and heart of newborn rats after 3 and 48 hours' exposure to anoxic hypoxia (10% O2) and after 48 hours' starvation. Control determinations were made on newborn animals of corresponding ages, full term foetuses (21 days), infantile (1 and 2 weeks) and full grown animals. Hypoxia for 3 h had no influence on succinate dehydrogenase activity at all in either the heart or liver mitochondria of the newborn animals. After 48 h no difference was observed in the liver between the hypoxic animals and the starved controls of the same age, though starvation itself had resulted in a significant increase in activity, as much as 42%. When liver mitochondrial succinate dehydrogenase in normal mitochondria was activated by preincubation mitochondria with the substrate, the activity increase obtained was greater than that resulting from starvation. The increase in activity in the heart of the hypoxic or starved animals was not significant (less than 10%).", "PMID": 961447}
{"id": "pubmed23n0032_4430", "title": "Vagal influence on serotonin concentration in enterochromaffin cells in the cat.", "content": "The intracellular concentrations of 5-HT in enterochromaffin cells of the jejunum, midgut and ileum of the cat have been estimated by a cytofluorimetric method before and after various periods of peripheral vagal nerve stimulation. The fluorescence intensities in randomized cell samples were measured photometrically after a standard illumination time before and after nerve stimulation. A significant decrease in 5-HT concentration in the enterochromaffin cells all through the small intestine could be demonstrated, which is in support of the hypothesis of a neurogenic release of 5-HT from enterochromaffin cells, possibly caused by vagal adrenergic fibres.", "contents": "Vagal influence on serotonin concentration in enterochromaffin cells in the cat. The intracellular concentrations of 5-HT in enterochromaffin cells of the jejunum, midgut and ileum of the cat have been estimated by a cytofluorimetric method before and after various periods of peripheral vagal nerve stimulation. The fluorescence intensities in randomized cell samples were measured photometrically after a standard illumination time before and after nerve stimulation. A significant decrease in 5-HT concentration in the enterochromaffin cells all through the small intestine could be demonstrated, which is in support of the hypothesis of a neurogenic release of 5-HT from enterochromaffin cells, possibly caused by vagal adrenergic fibres.", "PMID": 961448}
{"id": "pubmed23n0032_4431", "title": "Prostaglandins, a link between renal hydro- and hemodynamic in dogs.", "content": "An increase of the intrarenal pressure to 40 mmHg induced by ureteral constriction or by kidney compression is shown to be followed by increased renal blood flow in anesthetized dogs. This hyperemia is probably the result of enhanced  intrarenal prostaglandin activity since it is followed by increased urinary prostaglandin E excretion and is abolished by indomethacin pretreatment. The increase of renal blood flow seems to be due to dilation of the afferent arteriole in order to maintain the filtration pressure. The glomerular filtration rate is thus severely depressed in indomethacin pretreated dogs. Urine and electrolyte excretion is comparably redcued during elevated intrarenal pressure in non-pretreated and in indomethacin pretreated dogs, which suggests that factors other than glomerular filtration rate are involved. Urine osmolarity is positively correlated with renal blood flow, and urine osmolarity increases during elevated intrarenal pressure in non pretreated dogs, whilst urine osmolarity remains unchanged in dogs pretreated with indomethacin.", "contents": "Prostaglandins, a link between renal hydro- and hemodynamic in dogs. An increase of the intrarenal pressure to 40 mmHg induced by ureteral constriction or by kidney compression is shown to be followed by increased renal blood flow in anesthetized dogs. This hyperemia is probably the result of enhanced  intrarenal prostaglandin activity since it is followed by increased urinary prostaglandin E excretion and is abolished by indomethacin pretreatment. The increase of renal blood flow seems to be due to dilation of the afferent arteriole in order to maintain the filtration pressure. The glomerular filtration rate is thus severely depressed in indomethacin pretreated dogs. Urine and electrolyte excretion is comparably redcued during elevated intrarenal pressure in non-pretreated and in indomethacin pretreated dogs, which suggests that factors other than glomerular filtration rate are involved. Urine osmolarity is positively correlated with renal blood flow, and urine osmolarity increases during elevated intrarenal pressure in non pretreated dogs, whilst urine osmolarity remains unchanged in dogs pretreated with indomethacin.", "PMID": 961449}
{"id": "pubmed23n0032_4432", "title": "Effect of chronic sympathetic denervation upon local regulation of blood flow in human subcutaneous tissue.", "content": "The effect of chronic sympathetic denervation upon the vasoconstrictor response to an increase of vascular transmural pressure in human subcutaneous adipose tissue was investigated in 6 patients suffering from manual hyperhidrosis. Changes in transmural pressure were obtained either by postural changes of a forearm or by venous stasis of 30 mmHg. Blood flow was measured in the distal part of the forearm or crus by means of the local 133Xenon washout technique. 2 patients were studied before and after sympathectomy. When the area under study was lowered about 40 cm below the jugular notch, blood flow decreased about 50% preoperatively, about 30% 24 h after the operation, but remained constant 4 days after or later. Similar results were obtained during venous stasis. Hence about 4 days after sympathectomy, the vasoconstrictor response to an increase in vascular transmural pressure was abolished. In 3 chronically sympathectomized patients blood flow remained constant in the denervated limb, but decreased significantly in the control limb. In another patient studied 580 days after surgery blood flow remained constant during lowering of the denervated forearm as well as during venous stasis. These findings might indicate that the vasoconstrictor response to an increase in vascular transmural pressure in human subcutaneous adipose tissue is due to a local nervous mechanism involving symphathetic adrenergic nerves, but a myogenic mechanism cannot be definitively excluded.", "contents": "Effect of chronic sympathetic denervation upon local regulation of blood flow in human subcutaneous tissue. The effect of chronic sympathetic denervation upon the vasoconstrictor response to an increase of vascular transmural pressure in human subcutaneous adipose tissue was investigated in 6 patients suffering from manual hyperhidrosis. Changes in transmural pressure were obtained either by postural changes of a forearm or by venous stasis of 30 mmHg. Blood flow was measured in the distal part of the forearm or crus by means of the local 133Xenon washout technique. 2 patients were studied before and after sympathectomy. When the area under study was lowered about 40 cm below the jugular notch, blood flow decreased about 50% preoperatively, about 30% 24 h after the operation, but remained constant 4 days after or later. Similar results were obtained during venous stasis. Hence about 4 days after sympathectomy, the vasoconstrictor response to an increase in vascular transmural pressure was abolished. In 3 chronically sympathectomized patients blood flow remained constant in the denervated limb, but decreased significantly in the control limb. In another patient studied 580 days after surgery blood flow remained constant during lowering of the denervated forearm as well as during venous stasis. These findings might indicate that the vasoconstrictor response to an increase in vascular transmural pressure in human subcutaneous adipose tissue is due to a local nervous mechanism involving symphathetic adrenergic nerves, but a myogenic mechanism cannot be definitively excluded.", "PMID": 961450}
{"id": "pubmed23n0032_4433", "title": "Local nervous mechanism in regulation of blood flow in human subcutaneous tissue.", "content": "The effect of local venous stasis upon blood flow in human subcutaneous adipose tissue on the distal part of the forearm was investigated in three healthy subjects and two chronically sympathectomized patients suffering from manual hyperhidrosis. The area under study was separated into two parts by means of a lead shield exerting a pressure of about 360 mmHg on the skin. The effect of venous stasis of about 40 mmHg on one side of the shield upon blood flow measured simultaneously on both sides of the shield by the local 133Xenon washout technique was investigated. During venous stasis on one side of the shield, blood flow decreased about 40% on both sides. The vasoconstrictor impulse could be transmitted over a distance of about 1-2 cm. The phenomenon was unaffected by nerve blockade induced 3 cm proximally, medially, and laterally to the area by infiltration the skin with lidocaine. Thus a vasoconstrictor impulse could be transmitted from the side of stasis to the non stasis side of the lead shield. The transmission was not affected by phentolamine but was blocked by lidocaine and chronic sympathetic denervation. The vasoconstrictor impulse elicited during venous stasis is therefore most likely transmitted by means of a local nervous mechanism involving sympathetic adrenergic vasoconstrictor fibres.", "contents": "Local nervous mechanism in regulation of blood flow in human subcutaneous tissue. The effect of local venous stasis upon blood flow in human subcutaneous adipose tissue on the distal part of the forearm was investigated in three healthy subjects and two chronically sympathectomized patients suffering from manual hyperhidrosis. The area under study was separated into two parts by means of a lead shield exerting a pressure of about 360 mmHg on the skin. The effect of venous stasis of about 40 mmHg on one side of the shield upon blood flow measured simultaneously on both sides of the shield by the local 133Xenon washout technique was investigated. During venous stasis on one side of the shield, blood flow decreased about 40% on both sides. The vasoconstrictor impulse could be transmitted over a distance of about 1-2 cm. The phenomenon was unaffected by nerve blockade induced 3 cm proximally, medially, and laterally to the area by infiltration the skin with lidocaine. Thus a vasoconstrictor impulse could be transmitted from the side of stasis to the non stasis side of the lead shield. The transmission was not affected by phentolamine but was blocked by lidocaine and chronic sympathetic denervation. The vasoconstrictor impulse elicited during venous stasis is therefore most likely transmitted by means of a local nervous mechanism involving sympathetic adrenergic vasoconstrictor fibres.", "PMID": 961451}
{"id": "pubmed23n0032_4434", "title": "Diffuse hair loss in women: the psychopathology of those who complain.", "content": "Thirty-two women complaining of diffuse alopecia were examined by a psychiatrist. Seven of them were found to have severe, usually long-standing marital and sexual problems and two of them were overtly depressed. In a previous investigation the results of tests to assess the degree of hair loss of these seven women showed no significant difference from the values obtained in a control series of women. The psychopathology underlying this complaint is discussed and it is suggested that those women whose anxiety seems disproportionate to their degree of hair loss should be carefully questioned regarding depression and marital difficulties as these may cause them to be unusually sensitive to a hair loss which normally might not worry them sufficiently to seek further advice. They may then use their hair in a symbolic way to obtain help with their underlying problems.", "contents": "Diffuse hair loss in women: the psychopathology of those who complain. Thirty-two women complaining of diffuse alopecia were examined by a psychiatrist. Seven of them were found to have severe, usually long-standing marital and sexual problems and two of them were overtly depressed. In a previous investigation the results of tests to assess the degree of hair loss of these seven women showed no significant difference from the values obtained in a control series of women. The psychopathology underlying this complaint is discussed and it is suggested that those women whose anxiety seems disproportionate to their degree of hair loss should be carefully questioned regarding depression and marital difficulties as these may cause them to be unusually sensitive to a hair loss which normally might not worry them sufficiently to seek further advice. They may then use their hair in a symbolic way to obtain help with their underlying problems.", "PMID": 961453}
{"id": "pubmed23n0032_4435", "title": "Fat cell number and weight gain in lithium treated patients.", "content": "Total amount of fat, fat cell size, and fat cell number were determined in 33 patients under long-term lithium treatment. The weight gain during the treatment was estimated both by the patients themselves (questionnaire) and from their records. A positive correlation between fat cell number and weight gain was found whereas the fat cell size showed no correlation to weight gain.", "contents": "Fat cell number and weight gain in lithium treated patients. Total amount of fat, fat cell size, and fat cell number were determined in 33 patients under long-term lithium treatment. The weight gain during the treatment was estimated both by the patients themselves (questionnaire) and from their records. A positive correlation between fat cell number and weight gain was found whereas the fat cell size showed no correlation to weight gain.", "PMID": 961454}
{"id": "pubmed23n0032_4436", "title": "A ten-year follow-up of children of schizophrenic mothers. Clinical assessment.", "content": "A Group of 207 subjects with severely schizophrenic mothers and a matched group 104 subjects without known mental illness in the parents or grandparents have been followed since 1962 in a prospective study based on Mednick's learning theory of schizophrenia. The present paper describes an analysis of the clinical outcome based on a follow-up examination in 1972-1974. The examination consisted of a 3 1/2-hour clinical interview using three approaches (two with computer-derived diagnoses and one traditional clinical interview). 173 high-risk and 91 low-risk subjects were fully clinically reassessed. At the time of the assessment they were between 18 and 30 years old. The diagnostic distribution showed marked differences between the two groups with regard to type and degree of psychpathology. The results are discussed in relation to the methods used, and with regard to the case and non-case status of the subjects.", "contents": "A ten-year follow-up of children of schizophrenic mothers. Clinical assessment. A Group of 207 subjects with severely schizophrenic mothers and a matched group 104 subjects without known mental illness in the parents or grandparents have been followed since 1962 in a prospective study based on Mednick's learning theory of schizophrenia. The present paper describes an analysis of the clinical outcome based on a follow-up examination in 1972-1974. The examination consisted of a 3 1/2-hour clinical interview using three approaches (two with computer-derived diagnoses and one traditional clinical interview). 173 high-risk and 91 low-risk subjects were fully clinically reassessed. At the time of the assessment they were between 18 and 30 years old. The diagnostic distribution showed marked differences between the two groups with regard to type and degree of psychpathology. The results are discussed in relation to the methods used, and with regard to the case and non-case status of the subjects.", "PMID": 961455}
{"id": "pubmed23n0032_4437", "title": "Emotional disorders in six Israeli villages.", "content": "This is a report on a psychiatric epidemiological census conducted in six Israeli villages. The method of case identification relied on data extracted from medical cards and the use of two shortened versions of the CMI. The results showed a distribution affected by sex, age, ethnicity, education, and \"status consumer\". A comparison with other Israeli studies is made.", "contents": "Emotional disorders in six Israeli villages. This is a report on a psychiatric epidemiological census conducted in six Israeli villages. The method of case identification relied on data extracted from medical cards and the use of two shortened versions of the CMI. The results showed a distribution affected by sex, age, ethnicity, education, and \"status consumer\". A comparison with other Israeli studies is made.", "PMID": 961456}
{"id": "pubmed23n0032_4438", "title": "The pedophilic offender with antisocial character.", "content": "The purpose of the study was to clarify how pedophilic offenders with antisocial character (psychopathy) differ from \"ordinary\" pedophilic offenders, among whom there are many persons with immaturity and contact difficulties. For this purpose all pedophilia cases subjected to mental examination during a period of 25 years were investigated. Of these 81 cases, 38 could be regarded as antisocial characters on the basis of the current diagnostic criteria. The others were regarded as controls. The criminals with antisocial character had also been involved in other kinds of crimes and had had hyperactivity problems more often than controls. The controls were often immature, had a low intelligence, and were less able to have relations with adult people than were antisocial characters. However, according to the present results, these features might also make criminals with antisocial character more susceptible to pedophilia.", "contents": "The pedophilic offender with antisocial character. The purpose of the study was to clarify how pedophilic offenders with antisocial character (psychopathy) differ from \"ordinary\" pedophilic offenders, among whom there are many persons with immaturity and contact difficulties. For this purpose all pedophilia cases subjected to mental examination during a period of 25 years were investigated. Of these 81 cases, 38 could be regarded as antisocial characters on the basis of the current diagnostic criteria. The others were regarded as controls. The criminals with antisocial character had also been involved in other kinds of crimes and had had hyperactivity problems more often than controls. The controls were often immature, had a low intelligence, and were less able to have relations with adult people than were antisocial characters. However, according to the present results, these features might also make criminals with antisocial character more susceptible to pedophilia.", "PMID": 961457}
{"id": "pubmed23n0032_4439", "title": "A follow-up study of a child psychiatric clientele with special regard to the diagnosis of psychosis.", "content": "A 20-year follow-up of a child psychiatric clientele of 322 patients demonstrates that nearly one third have been admitted to psychiatric departments or mental hospitals in adulthood. One tenth belonged to the group with psychoses either as a child or grown-up. While the incidence of manic-depressive psychosis did not differ from a normal population of the same sex and age, the child psychiatric clientele is overrepresented by psychotic patients later on diagnosed as schizophrenia. The outcome of infantile psychosis was in half of the cases chronic psychosis; five of 10 psychosis proto-infantilis patients were diagnosed schizophrenia in adulthood. This result is not in accordance with the modern view that psychosis proto-infantilis is a special disease with no clinical connection to schizophrenia. The clinical entity of infantile psychosis and borderline psychosis seems to be affirmed by a common clinical and diagnostic course into borderline psychosis or schizoid character disorders. Nine patients with psychosis in adulthood did not belong to the group of psychosis in childhood.", "contents": "A follow-up study of a child psychiatric clientele with special regard to the diagnosis of psychosis. A 20-year follow-up of a child psychiatric clientele of 322 patients demonstrates that nearly one third have been admitted to psychiatric departments or mental hospitals in adulthood. One tenth belonged to the group with psychoses either as a child or grown-up. While the incidence of manic-depressive psychosis did not differ from a normal population of the same sex and age, the child psychiatric clientele is overrepresented by psychotic patients later on diagnosed as schizophrenia. The outcome of infantile psychosis was in half of the cases chronic psychosis; five of 10 psychosis proto-infantilis patients were diagnosed schizophrenia in adulthood. This result is not in accordance with the modern view that psychosis proto-infantilis is a special disease with no clinical connection to schizophrenia. The clinical entity of infantile psychosis and borderline psychosis seems to be affirmed by a common clinical and diagnostic course into borderline psychosis or schizoid character disorders. Nine patients with psychosis in adulthood did not belong to the group of psychosis in childhood.", "PMID": 961458}
{"id": "pubmed23n0032_4440", "title": "Controlled trial of penfluridol and thiothixene in the maintenance treatment of chronic schizophrenic syndromes.", "content": "In a controlled trial of penfluridol and thiothixene as maintenance drugs in patients with chronic schizophrenic syndromes, some improvement over previous neuroleptics was seen with both drugs. This improvement was mainly evident in variables concerned with participation in social activities as assessed with the S-scale and by ward behaviour. The drug dosages necessary were very low and gave few and easily manageable side-effects. There was no significant difference between penfluridol and thiothixene. Penfluridol has the clear practical advantage of being the only long-acting drug for oral administration so far available.", "contents": "Controlled trial of penfluridol and thiothixene in the maintenance treatment of chronic schizophrenic syndromes. In a controlled trial of penfluridol and thiothixene as maintenance drugs in patients with chronic schizophrenic syndromes, some improvement over previous neuroleptics was seen with both drugs. This improvement was mainly evident in variables concerned with participation in social activities as assessed with the S-scale and by ward behaviour. The drug dosages necessary were very low and gave few and easily manageable side-effects. There was no significant difference between penfluridol and thiothixene. Penfluridol has the clear practical advantage of being the only long-acting drug for oral administration so far available.", "PMID": 961459}
{"id": "pubmed23n0032_4441", "title": "Psychopathology, feelings of confinement and helplessness in the dental chair, and relationship to the dentist in patients with disproportionate dental anxiety (DDA).", "content": "Nineteen individuals with inordinate fear of dental treatment are presented and discussed with respect to their psychopathology and those aspects of their fear involving feelings of confinement and helplessness in the dental chair, and a negative relationship with the dentist. This material is part of a comprehensive investigation previously reported by the authors into the components and factors contributing to this kind of fear, and the personality and emotional reactions of the individuals suffering from it. The data presented are based on single structured interviews of each patient. Analysis of the patient population suggests division into four major categories: those in whom fear of dental treatment was associated with feelings of inferiority in bodily appearance or function (the largest category); those in whom the fear was associated with neurotic disturbances in which disturbance in body image is not apparent; those in whom the  fear was a reflection of a schizophrenic or schizoid process; and those in whom no overt psychopathology was found. The patients in the first category were those who tended most to be afraid of dental treatment due to feelings of confinement or helplessness in the dental chair, or due to a negative relationship with the dentist.", "contents": "Psychopathology, feelings of confinement and helplessness in the dental chair, and relationship to the dentist in patients with disproportionate dental anxiety (DDA). Nineteen individuals with inordinate fear of dental treatment are presented and discussed with respect to their psychopathology and those aspects of their fear involving feelings of confinement and helplessness in the dental chair, and a negative relationship with the dentist. This material is part of a comprehensive investigation previously reported by the authors into the components and factors contributing to this kind of fear, and the personality and emotional reactions of the individuals suffering from it. The data presented are based on single structured interviews of each patient. Analysis of the patient population suggests division into four major categories: those in whom fear of dental treatment was associated with feelings of inferiority in bodily appearance or function (the largest category); those in whom the fear was associated with neurotic disturbances in which disturbance in body image is not apparent; those in whom the  fear was a reflection of a schizophrenic or schizoid process; and those in whom no overt psychopathology was found. The patients in the first category were those who tended most to be afraid of dental treatment due to feelings of confinement or helplessness in the dental chair, or due to a negative relationship with the dentist.", "PMID": 961461}
{"id": "pubmed23n0032_4442", "title": "An analysis of homicide by young persons in England and Wales.", "content": "An analysis of 11 children (one girl, 10 boys) convicted of homicide indicates a maternal over-dominant relationship in eight of the males studied. The murderers were found to have more cooperative personalities than other children found guilty of non-capital offences and showed both normal intelligence and personality factors. Despite a history of \"blackouts\" in several cases, all were found on investigation to be free from both major and minor epilepsy. Difficulty was experienced in determining the presence or absence of parental alcoholism, promiscuity, and criminal convictions. All of the children came from normal homes and apart from minor offences none had been involved previously in serious antisocial behaviour. Future research is indicated on two aspects: (a) Role of the working mother with particular reference to maternal dominance and (b) The \"Cycle of Deprivation\" theory.", "contents": "An analysis of homicide by young persons in England and Wales. An analysis of 11 children (one girl, 10 boys) convicted of homicide indicates a maternal over-dominant relationship in eight of the males studied. The murderers were found to have more cooperative personalities than other children found guilty of non-capital offences and showed both normal intelligence and personality factors. Despite a history of \"blackouts\" in several cases, all were found on investigation to be free from both major and minor epilepsy. Difficulty was experienced in determining the presence or absence of parental alcoholism, promiscuity, and criminal convictions. All of the children came from normal homes and apart from minor offences none had been involved previously in serious antisocial behaviour. Future research is indicated on two aspects: (a) Role of the working mother with particular reference to maternal dominance and (b) The \"Cycle of Deprivation\" theory.", "PMID": 961462}
{"id": "pubmed23n0032_4443", "title": "Effect of flupenthixol on depression with special reference to combination use with tricyclic antidepressants. An uncontrolled pilot study with 45 patients.", "content": "In an open, uncontrolled trial flupenthixol was administered to 45 patients with endogenous depression. The drug was markedly effective in eight patients, effective in nine patients, fairly effective in 12 patients, and ineffective or aggravating in 16 patients. Four patients showed transient manic symptoms. Dosage was 1-3 mg daily. In 36 patients flupenthixol was used in combination with previously administered tricyclic antidepressants, and in nine patients it was used alone. Clinical effect was quickly apparent. It appeared within 1 week in 63% and within 2 weeks in 93% of subjects. Side-effects were observed in 13 patients: insomnia, five patients; slight extrapyramidal symptoms, nine patients. Sedative-hypnogenic effects were rarely seen. In 71% of 17 patients in whom the drug was found to be markedly effective or effective, flupenthixol's influence on psychomotor retardation was particularly striking. Other clear benefits were relief of depressive mood, psychic anxiety, and agitation. It is recommended that flupenthixol is given, as supplementary medication, to patients (1) whose depressive symptoms other than psychomotor retardation have already improved with current tricyclic antidepressants, and (2) in whom, before antidepressant medication, psychomotor retardation is a principal feature.", "contents": "Effect of flupenthixol on depression with special reference to combination use with tricyclic antidepressants. An uncontrolled pilot study with 45 patients. In an open, uncontrolled trial flupenthixol was administered to 45 patients with endogenous depression. The drug was markedly effective in eight patients, effective in nine patients, fairly effective in 12 patients, and ineffective or aggravating in 16 patients. Four patients showed transient manic symptoms. Dosage was 1-3 mg daily. In 36 patients flupenthixol was used in combination with previously administered tricyclic antidepressants, and in nine patients it was used alone. Clinical effect was quickly apparent. It appeared within 1 week in 63% and within 2 weeks in 93% of subjects. Side-effects were observed in 13 patients: insomnia, five patients; slight extrapyramidal symptoms, nine patients. Sedative-hypnogenic effects were rarely seen. In 71% of 17 patients in whom the drug was found to be markedly effective or effective, flupenthixol's influence on psychomotor retardation was particularly striking. Other clear benefits were relief of depressive mood, psychic anxiety, and agitation. It is recommended that flupenthixol is given, as supplementary medication, to patients (1) whose depressive symptoms other than psychomotor retardation have already improved with current tricyclic antidepressants, and (2) in whom, before antidepressant medication, psychomotor retardation is a principal feature.", "PMID": 961463}
{"id": "pubmed23n0032_4444", "title": "L-alpha-hydroxycholecalciferol treatment of adults with chronic renal failure.", "content": "Five adult patients with chronic renal failure and associated renal osteodystrophy have been treated for 6 months with 1-alpha-hydroxycholecalciferol (1 alpha-OH-D3), a synthetic vitamin D analogue. All 5 patients had severe metabolic bone changes as estimated by bone scintigraphy. Three patients were hypocalcemic, 4 had elevated serum alkaline phosphatases, 5 had elevated serum immunoreactive parathyroid hormone (i-PTH) concentration and 3 had bone pains. During treatment serum calcium increased in all patients (mean 11.4%) and 3 originally hypocalcemic patients became normocalcemic. Serum alkaline phosphatases decreased (mean 27.3%) and became normal in 4 patients, who initially had elevated values. A pronounced decline in the serum concentration of i-PTH (mean 53%) was seen in all patients and 1 patient obtained normal i-PTH levels after 4 months of treatment. The intestinal calcium absorption, which was low initially, even when calcium intake was considered, rose almost threefold (mean 273%) and reached normal values in all cases. The bone mineral content increased in all patients, but the changes were small (mean 4.9%) and insignificant. Finally, bone pain disappeared in 2 patients and improved in 1 of 3 patients exhibiting this symptom. A linear correlation (r = 0.48, p less than 0.001) was found between the dose of 1 alpha-OH-D3 and serum calcium. But in spite of this and the frequent control, all patients developed one episode of hypercalcemia. This disappeared within 48 hours after discontinuing the drug. It is concluded that treatment with 1 alpha-OH-D3 appears to be of therapeutic value in metabolic bone disease associated with chronic renal failure, but frequent control of blood biochemistry seems mandatory.", "contents": "L-alpha-hydroxycholecalciferol treatment of adults with chronic renal failure. Five adult patients with chronic renal failure and associated renal osteodystrophy have been treated for 6 months with 1-alpha-hydroxycholecalciferol (1 alpha-OH-D3), a synthetic vitamin D analogue. All 5 patients had severe metabolic bone changes as estimated by bone scintigraphy. Three patients were hypocalcemic, 4 had elevated serum alkaline phosphatases, 5 had elevated serum immunoreactive parathyroid hormone (i-PTH) concentration and 3 had bone pains. During treatment serum calcium increased in all patients (mean 11.4%) and 3 originally hypocalcemic patients became normocalcemic. Serum alkaline phosphatases decreased (mean 27.3%) and became normal in 4 patients, who initially had elevated values. A pronounced decline in the serum concentration of i-PTH (mean 53%) was seen in all patients and 1 patient obtained normal i-PTH levels after 4 months of treatment. The intestinal calcium absorption, which was low initially, even when calcium intake was considered, rose almost threefold (mean 273%) and reached normal values in all cases. The bone mineral content increased in all patients, but the changes were small (mean 4.9%) and insignificant. Finally, bone pain disappeared in 2 patients and improved in 1 of 3 patients exhibiting this symptom. A linear correlation (r = 0.48, p less than 0.001) was found between the dose of 1 alpha-OH-D3 and serum calcium. But in spite of this and the frequent control, all patients developed one episode of hypercalcemia. This disappeared within 48 hours after discontinuing the drug. It is concluded that treatment with 1 alpha-OH-D3 appears to be of therapeutic value in metabolic bone disease associated with chronic renal failure, but frequent control of blood biochemistry seems mandatory.", "PMID": 961464}
{"id": "pubmed23n0032_4445", "title": "Three-year follow-up of middle-aged men with low blood pressure.", "content": "The blood pressure (BP) development in 142 middle-aged men with BP within the lower region of the BP distribution (supine BP less than or equal to 120/70 mmHg) has been studied. Over a 3-year period there was a moderate but significant rise in supine systolic BP but not in supine diastolic BP. However, no subject developed hypertension defined as supine BP greater than or equal to 175/105 mmHg. These findings support the assumption that among middle-aged men the hypertensives are recruited from the upper part of the BP distribution. Subjects with low BP may be rescreened at longer intervals, if at all.", "contents": "Three-year follow-up of middle-aged men with low blood pressure. The blood pressure (BP) development in 142 middle-aged men with BP within the lower region of the BP distribution (supine BP less than or equal to 120/70 mmHg) has been studied. Over a 3-year period there was a moderate but significant rise in supine systolic BP but not in supine diastolic BP. However, no subject developed hypertension defined as supine BP greater than or equal to 175/105 mmHg. These findings support the assumption that among middle-aged men the hypertensives are recruited from the upper part of the BP distribution. Subjects with low BP may be rescreened at longer intervals, if at all.", "PMID": 961465}
{"id": "pubmed23n0032_4446", "title": "Attempted diagnosis of ventricular mural thrombi in acute mycardial infarction using 125I-labelled fibrinogen.", "content": "In an attempt to diagnose ventricular mural thrombi complicating acute myocardial infarction (AMI), 80 patients have been given 100 muCi 125I-labelled fibrinogen after admission to a CCU. Precordial radioactivity was recorded for the following 6 days over four sites corresponding to chest leads CR1-CR4. A sustained rise in radioactivity of at least 15% of initial recordings was classed as type A pattern, a minor rise or flattened response as type B pattern and a rapid decrease as type C pattern; 28% showed a type A, 19% a type B and 54% a type C pattern. There was no significant difference between the groups in incidence of pericardial friction rub but when patients with suspected pericarditis (as evidenced by characteristic pains) were added, pericarditis was significantly overrepresented in the type A group. Smaller infarctions (SGOT less than 100 U/1) were significantly more common in patients with a type C decay pattern. No differences were noted between the groups as regards type and site of the infarction. A sustained rise in precordial radioactivity after an AMI may be an indication of mural thrombosis but the influence of other factors secondary to an infarction, e.g. pericarditis, cannot be determined at present.", "contents": "Attempted diagnosis of ventricular mural thrombi in acute mycardial infarction using 125I-labelled fibrinogen. In an attempt to diagnose ventricular mural thrombi complicating acute myocardial infarction (AMI), 80 patients have been given 100 muCi 125I-labelled fibrinogen after admission to a CCU. Precordial radioactivity was recorded for the following 6 days over four sites corresponding to chest leads CR1-CR4. A sustained rise in radioactivity of at least 15% of initial recordings was classed as type A pattern, a minor rise or flattened response as type B pattern and a rapid decrease as type C pattern; 28% showed a type A, 19% a type B and 54% a type C pattern. There was no significant difference between the groups in incidence of pericardial friction rub but when patients with suspected pericarditis (as evidenced by characteristic pains) were added, pericarditis was significantly overrepresented in the type A group. Smaller infarctions (SGOT less than 100 U/1) were significantly more common in patients with a type C decay pattern. No differences were noted between the groups as regards type and site of the infarction. A sustained rise in precordial radioactivity after an AMI may be an indication of mural thrombosis but the influence of other factors secondary to an infarction, e.g. pericarditis, cannot be determined at present.", "PMID": 961466}
{"id": "pubmed23n0032_4447", "title": "Prevalence of hypercalcaemia in a health screening in Stockholm.", "content": "A free health check, offered to 21417 20-63-year-old employees of the Stockholm City and County Council in 1971-73, was accepted by 15903 persons. The examination included a multichannel chemical analysis of a single blood sample. Serum calcium levels greater than or equal to 11.0 mg/100 ml (2.75 mmol/l) and greater than or equal to 11.1 mg/100 ml (2.78 mmol/l) were encountered in 3.9% and 1.1% of the population, respectively. Among subjects below 50 years of age, the calcium concentration was significantly higher in males than in females. This difference disappeared in older subjects, essentially because the calcium level decreased with advancing age in the men. To a further investigation were invited 178 subjects with a single serum calcium registration greater than or equal to 11.1 mg/100 ml (2.78 mmol/l). Of this group, 95 persons (53.4%) exhibited hypercalcaemia (HC) on repeated testing. Twelve had been operated on prior to the actural follow-up and found to have parathyroid adenomata. Twenty subjects were on continuous treatment with diuretics of the thiazide type and seven had diseases that might induce HC (two had hyperthyroidism, two hypothyroidism, one sarcoidosis, one hypernephroma and one mammary carcinoma). In 56 patients the laboratory and physical examinations did not reveal any obvious cause for the HC except possible hyperparathyoidism (HPT). Eighty (84.2%) of the 95 HC subjects were women, mostly over 50 years. The 95 persons constituted 6% of the total number of health-screened persons. The highest prevalence, 13%, was recorded for women aged 60-63. The prevalence of HPT in the total material was 3.6%, which is higher than that found in several other studies. This is based on surgical findings to date.", "contents": "Prevalence of hypercalcaemia in a health screening in Stockholm. A free health check, offered to 21417 20-63-year-old employees of the Stockholm City and County Council in 1971-73, was accepted by 15903 persons. The examination included a multichannel chemical analysis of a single blood sample. Serum calcium levels greater than or equal to 11.0 mg/100 ml (2.75 mmol/l) and greater than or equal to 11.1 mg/100 ml (2.78 mmol/l) were encountered in 3.9% and 1.1% of the population, respectively. Among subjects below 50 years of age, the calcium concentration was significantly higher in males than in females. This difference disappeared in older subjects, essentially because the calcium level decreased with advancing age in the men. To a further investigation were invited 178 subjects with a single serum calcium registration greater than or equal to 11.1 mg/100 ml (2.78 mmol/l). Of this group, 95 persons (53.4%) exhibited hypercalcaemia (HC) on repeated testing. Twelve had been operated on prior to the actural follow-up and found to have parathyroid adenomata. Twenty subjects were on continuous treatment with diuretics of the thiazide type and seven had diseases that might induce HC (two had hyperthyroidism, two hypothyroidism, one sarcoidosis, one hypernephroma and one mammary carcinoma). In 56 patients the laboratory and physical examinations did not reveal any obvious cause for the HC except possible hyperparathyoidism (HPT). Eighty (84.2%) of the 95 HC subjects were women, mostly over 50 years. The 95 persons constituted 6% of the total number of health-screened persons. The highest prevalence, 13%, was recorded for women aged 60-63. The prevalence of HPT in the total material was 3.6%, which is higher than that found in several other studies. This is based on surgical findings to date.", "PMID": 961467}
{"id": "pubmed23n0032_4448", "title": "Extensive nodular infiltration of extra-osseous tissues in human myelomatosis. A case report.", "content": "The autopsy findings of a woman with myelomatosis diagnosed half a year before her death are described. She had an IgG-lambda myeloma which initially responded to treatment. However, subsequently she developed a condition characterized by massive soft tissue involvement with increased number of plasma cells in peripheral blood. Rounded hard myeloma infiltrates were disseminated through the body, only sparing the adrenal glands and intracranial structures.", "contents": "Extensive nodular infiltration of extra-osseous tissues in human myelomatosis. A case report. The autopsy findings of a woman with myelomatosis diagnosed half a year before her death are described. She had an IgG-lambda myeloma which initially responded to treatment. However, subsequently she developed a condition characterized by massive soft tissue involvement with increased number of plasma cells in peripheral blood. Rounded hard myeloma infiltrates were disseminated through the body, only sparing the adrenal glands and intracranial structures.", "PMID": 961468}
{"id": "pubmed23n0032_4449", "title": "Prolonged symptoms of brain dysfunction--adverse effect of levodopa.", "content": "Symptoms of brain dysfunction occurred in a 67-year-old woman with idiopathic parkinsonism on treatment with levodopa. The adverse effect reappeared in a more severe and prolonged form when she was treated one year later with levodopa in combination with the peripheral decarboxylase inhibitor Ro-4-4602. The symptoms were associated with a markedly altered level of HMPG (a noradrenaline metabolite) in the cerebrospinal fluid.", "contents": "Prolonged symptoms of brain dysfunction--adverse effect of levodopa. Symptoms of brain dysfunction occurred in a 67-year-old woman with idiopathic parkinsonism on treatment with levodopa. The adverse effect reappeared in a more severe and prolonged form when she was treated one year later with levodopa in combination with the peripheral decarboxylase inhibitor Ro-4-4602. The symptoms were associated with a markedly altered level of HMPG (a noradrenaline metabolite) in the cerebrospinal fluid.", "PMID": 961469}
{"id": "pubmed23n0032_4450", "title": "Studies on subclinical hypothyroidism with special reference to the serum lipid pattern.", "content": "Subclinical hypothyroidism is an example of the impact of technology on the concept of a disease. It denotes a condition in which laboratory findings, at least including a raised serum thyrotropin (s-TSH), indicate hypothyroidism in the absence of clinical signs or symptoms of this disease. One reason for attention to cases of subclinical hypothyroidism is the publication of reports, from the time before introduction of the s-TSH assay, that hypercholesterolaemia precedes other evidence of thyroid failure with attendant risks of ischaemic heart disease and other atherosclerotic manifestations. The present investigation, which concerned the lipid pattern in sublinical hypothyroiism, offered no support for such a concept of hypercholesterolaemia as a premonitory sign of hypothyroidism. Furthermore, no significant differences were found between the serum levels of cholesterol and triglycerides before and after the administration of a thyroxine dose, necessary to suppress the s-TSH into a normal range, in cases of sublinical hypothyroidism. Nor were there any changes during this therapy in body weight, ECG, or Hb levels, which represent important parameters often found to be abnormal in overt hypothyroidism. From a practical point of view, subclinical hypothyroidism probably can be regarded as a state in which reduction of thyroid activity has been compensated by an increased s-TSH secretion to maintain a clinically euthyroid state. When no goitre is found, the rationale of treatment of this condition remains to be proved.", "contents": "Studies on subclinical hypothyroidism with special reference to the serum lipid pattern. Subclinical hypothyroidism is an example of the impact of technology on the concept of a disease. It denotes a condition in which laboratory findings, at least including a raised serum thyrotropin (s-TSH), indicate hypothyroidism in the absence of clinical signs or symptoms of this disease. One reason for attention to cases of subclinical hypothyroidism is the publication of reports, from the time before introduction of the s-TSH assay, that hypercholesterolaemia precedes other evidence of thyroid failure with attendant risks of ischaemic heart disease and other atherosclerotic manifestations. The present investigation, which concerned the lipid pattern in sublinical hypothyroiism, offered no support for such a concept of hypercholesterolaemia as a premonitory sign of hypothyroidism. Furthermore, no significant differences were found between the serum levels of cholesterol and triglycerides before and after the administration of a thyroxine dose, necessary to suppress the s-TSH into a normal range, in cases of sublinical hypothyroidism. Nor were there any changes during this therapy in body weight, ECG, or Hb levels, which represent important parameters often found to be abnormal in overt hypothyroidism. From a practical point of view, subclinical hypothyroidism probably can be regarded as a state in which reduction of thyroid activity has been compensated by an increased s-TSH secretion to maintain a clinically euthyroid state. When no goitre is found, the rationale of treatment of this condition remains to be proved.", "PMID": 961470}
{"id": "pubmed23n0032_4451", "title": "The composition of food consumed by Greenland Eskimos.", "content": "Food specimens have been collected, by means of the double-portion technique, from Greenland Eskimo hunters and their wives, in all seven persons, on seven consecutive days. Their food was found to contain more protein and less carbohydrates than average Danish food and an almost equal amount of fat. Compared with Danish food, the fatty acid pattern of the consumed lipids--essentially of mammalian marine origin--showed a higher content of long chain polyunsaturated fatty acids (especially C20:5) and lower contents of linoleic and linolenic acids. However, the sum of the polyunsaturated fatty acids was smaller than in Danish food. Using Keys' formula, describing the serum cholesterol level as a function of the nutritional fatty acids, the essentially lower serum choelsterol level found in Greenland Eskimos was not explained by our findings. It is suggested instead to be a special metabolic effect of the long chain polyunsaturated fatty acids from marine mammals. There might be a similar effect on the plasma triglyceride and very low density lipoprotein concentrations, explaining the much lower plasma concentrations of these components in Eskimos than in Western populations. Our findings might have an essential bearing on the difference in morbidity from coronary atherosclerotic disease between these populations.", "contents": "The composition of food consumed by Greenland Eskimos. Food specimens have been collected, by means of the double-portion technique, from Greenland Eskimo hunters and their wives, in all seven persons, on seven consecutive days. Their food was found to contain more protein and less carbohydrates than average Danish food and an almost equal amount of fat. Compared with Danish food, the fatty acid pattern of the consumed lipids--essentially of mammalian marine origin--showed a higher content of long chain polyunsaturated fatty acids (especially C20:5) and lower contents of linoleic and linolenic acids. However, the sum of the polyunsaturated fatty acids was smaller than in Danish food. Using Keys' formula, describing the serum cholesterol level as a function of the nutritional fatty acids, the essentially lower serum choelsterol level found in Greenland Eskimos was not explained by our findings. It is suggested instead to be a special metabolic effect of the long chain polyunsaturated fatty acids from marine mammals. There might be a similar effect on the plasma triglyceride and very low density lipoprotein concentrations, explaining the much lower plasma concentrations of these components in Eskimos than in Western populations. Our findings might have an essential bearing on the difference in morbidity from coronary atherosclerotic disease between these populations.", "PMID": 961471}
{"id": "pubmed23n0032_4452", "title": "Reduction of isoniazid bioavailability in normal men by concomitant intake of food.", "content": "The influence of food intake on the bioavailability of isoniazid (INH) has been examined in nine healthy male volunteers. INH was administered as a single oral dose, both in fasting state and together with a standardized breakfast. Numerous venous blood samples were obtained 5 min-6 hours after the INH ingestion, and the concentrations of unmetabolized INH in serum were assessed by spectrophotometry. The observations indicate that both the peak concentration and the total amount of INH absorbed are greatly reduced when the drug is ingested together with food. Hence it is recommended that, in the treatment of tuberculosis with INH, the drug should be given on an empty stomach. The data may also have some bearing on the use of INH for assessing acetylation rates and estimating dosages of hydralazine and related drugs.", "contents": "Reduction of isoniazid bioavailability in normal men by concomitant intake of food. The influence of food intake on the bioavailability of isoniazid (INH) has been examined in nine healthy male volunteers. INH was administered as a single oral dose, both in fasting state and together with a standardized breakfast. Numerous venous blood samples were obtained 5 min-6 hours after the INH ingestion, and the concentrations of unmetabolized INH in serum were assessed by spectrophotometry. The observations indicate that both the peak concentration and the total amount of INH absorbed are greatly reduced when the drug is ingested together with food. Hence it is recommended that, in the treatment of tuberculosis with INH, the drug should be given on an empty stomach. The data may also have some bearing on the use of INH for assessing acetylation rates and estimating dosages of hydralazine and related drugs.", "PMID": 961472}
{"id": "pubmed23n0032_4453", "title": "Lactate production during fructose infusion with or without amino acids.", "content": "Lactate production from the liver during fructose infusion was decreased when an amino acid infusion was given simultaneously. The most pronounced decrease was observed when the amino acid infusion was started before the simultaneous administration of fructose and amino acids. The explanation of the phenomenon is thought to be a stimulation of gluconeogenesis by amino acids.", "contents": "Lactate production during fructose infusion with or without amino acids. Lactate production from the liver during fructose infusion was decreased when an amino acid infusion was given simultaneously. The most pronounced decrease was observed when the amino acid infusion was started before the simultaneous administration of fructose and amino acids. The explanation of the phenomenon is thought to be a stimulation of gluconeogenesis by amino acids.", "PMID": 961473}
{"id": "pubmed23n0032_4454", "title": "On the nature of brain stem disorders in severe head injured patients.", "content": "In head injured patients changes were found in the CSF levels of metabolites of the dopaminergic and the serotonergic neurotransmission (HVA and 5-HIAA). After the fifth day following trauma a significant decrease of the HVA levels in the lumbar CSF after probenecid treatment was found. The intensity of this decrease was found to be related to the severity of the trauma (period of unconsciousness), but not to the state of consciousness. Also the probenecid-induced 5-HIAA levels in conscious patients were decreased after the fifth post-traumatic day. In unconscious patients, the period of unconsciousness, particularly in the period of 5-20 days after trauma. Between 21 and 60 days after trauma the 5-HIAA concentrations in unconscious patients decreased remarkably, but they were still significantly higher than in the conscious patients. It can be concluded that during unconsciousness serotonergic neurons have a high rate of turnover, but that both neurotransmitter systems are damaged by the direct or indirect consequences of the injury.", "contents": "On the nature of brain stem disorders in severe head injured patients. In head injured patients changes were found in the CSF levels of metabolites of the dopaminergic and the serotonergic neurotransmission (HVA and 5-HIAA). After the fifth day following trauma a significant decrease of the HVA levels in the lumbar CSF after probenecid treatment was found. The intensity of this decrease was found to be related to the severity of the trauma (period of unconsciousness), but not to the state of consciousness. Also the probenecid-induced 5-HIAA levels in conscious patients were decreased after the fifth post-traumatic day. In unconscious patients, the period of unconsciousness, particularly in the period of 5-20 days after trauma. Between 21 and 60 days after trauma the 5-HIAA concentrations in unconscious patients decreased remarkably, but they were still significantly higher than in the conscious patients. It can be concluded that during unconsciousness serotonergic neurons have a high rate of turnover, but that both neurotransmitter systems are damaged by the direct or indirect consequences of the injury.", "PMID": 961474}
{"id": "pubmed23n0032_4455", "title": "Diagnosis of pinealoblastomas by positive response to cobalt-therapy.", "content": "Surgical excision of pineoblastomas is considered dangerous, and relief of raised intracranial pressure by shunting followed by cobalt therapy is the preferred treatment. Demonstration by pneumoencephalography of tumour shrinkage after therapy confirms the diagnosis of pineoblastoma. Failure of the tumour to become smaller indicates a different pathology which calls for a surgical approach, except in the case of a brain stem tumour.", "contents": "Diagnosis of pinealoblastomas by positive response to cobalt-therapy. Surgical excision of pineoblastomas is considered dangerous, and relief of raised intracranial pressure by shunting followed by cobalt therapy is the preferred treatment. Demonstration by pneumoencephalography of tumour shrinkage after therapy confirms the diagnosis of pineoblastoma. Failure of the tumour to become smaller indicates a different pathology which calls for a surgical approach, except in the case of a brain stem tumour.", "PMID": 961475}
{"id": "pubmed23n0032_4456", "title": "Diagnosis and treatment of tumours in the pineal region.", "content": "In a series of 3.072 cases of intracranial neoplasms up to the end of 1973 there were 82 tumours in the pineal region (2.7%), excluding meningiomas of the falx and tentorium. More than half (forty seven) were pinealomas (two cell pattern type tumours, and pineoblastomas), twelve were teratomas, eight were ependymomas, three were epidermoids, and the remainder were gliomas. In addition to ventriculography, millipore filter-cell culture of cerebrospinal fluid, and fibre ventriculoscopy were found useful for diagnosis. Most of the patients were treated by surgery followed by radiotherapy. Results with the pinealomas were fairly good, the one year, five year, and ten year survival rates being 87.5%, 71.4%, and 33.3% in those below the age of 15 years, and 80.0%, 46.2%, and 12.5% in those above that age. The radiosensitivity of pinealomas and the beneficial effect of reduction of tumour bulk by direct surgery may both be important factors in the achievement of good results.", "contents": "Diagnosis and treatment of tumours in the pineal region. In a series of 3.072 cases of intracranial neoplasms up to the end of 1973 there were 82 tumours in the pineal region (2.7%), excluding meningiomas of the falx and tentorium. More than half (forty seven) were pinealomas (two cell pattern type tumours, and pineoblastomas), twelve were teratomas, eight were ependymomas, three were epidermoids, and the remainder were gliomas. In addition to ventriculography, millipore filter-cell culture of cerebrospinal fluid, and fibre ventriculoscopy were found useful for diagnosis. Most of the patients were treated by surgery followed by radiotherapy. Results with the pinealomas were fairly good, the one year, five year, and ten year survival rates being 87.5%, 71.4%, and 33.3% in those below the age of 15 years, and 80.0%, 46.2%, and 12.5% in those above that age. The radiosensitivity of pinealomas and the beneficial effect of reduction of tumour bulk by direct surgery may both be important factors in the achievement of good results.", "PMID": 961476}
{"id": "pubmed23n0032_4457", "title": "Surgical management of tumours of the pineal region.", "content": "Tumours of the pineal region and posterior part of the third ventricle are rare in European and American series (0.5 to 1% of space-occupying lesions). Personal experience is usually limited and thus cooperative studies or critical reviews of reported cases are necessary to establish therapeutic criteria. We have studied 50 cases from our own material of more than 5,000 brain tumours and intracranial space-occupying lesions operated on during the last 30 years. Another 6 verified cases from Dr. F. Isamat, and more than 200 verified tumours from the literature are added. Histological distribution shows that 24% of the tumours in this locality are benign. The different surgical techniques fro reaching the pineal region are reviewed. The results of palliative operations, or partial removals together with radiotherapy, and total removals are presented in 200 verified cases (Table 1), as well as the results obtained by the different surgical approaches (Table 2). Finally, some conclusions are reached regarding the surgical management of these tumours. More than two thirds are radiosensitive, and in about 50 to 70% good results may be obtained with palliative operations and radiotherapy. The remainder of the pineal tumours (about 20%) should be treated by a direct surgical approach. Recent technical improvements and the use of the surgical microscope have reduced the mortality of pineal surgery and allow verification of the histological nature of the tumour in order to establish more rational treatment.", "contents": "Surgical management of tumours of the pineal region. Tumours of the pineal region and posterior part of the third ventricle are rare in European and American series (0.5 to 1% of space-occupying lesions). Personal experience is usually limited and thus cooperative studies or critical reviews of reported cases are necessary to establish therapeutic criteria. We have studied 50 cases from our own material of more than 5,000 brain tumours and intracranial space-occupying lesions operated on during the last 30 years. Another 6 verified cases from Dr. F. Isamat, and more than 200 verified tumours from the literature are added. Histological distribution shows that 24% of the tumours in this locality are benign. The different surgical techniques fro reaching the pineal region are reviewed. The results of palliative operations, or partial removals together with radiotherapy, and total removals are presented in 200 verified cases (Table 1), as well as the results obtained by the different surgical approaches (Table 2). Finally, some conclusions are reached regarding the surgical management of these tumours. More than two thirds are radiosensitive, and in about 50 to 70% good results may be obtained with palliative operations and radiotherapy. The remainder of the pineal tumours (about 20%) should be treated by a direct surgical approach. Recent technical improvements and the use of the surgical microscope have reduced the mortality of pineal surgery and allow verification of the histological nature of the tumour in order to establish more rational treatment.", "PMID": 961477}
{"id": "pubmed23n0032_4458", "title": "Quantitative study of muscle fibre atrophy and restitution after nerve grafts.", "content": "Our comparative experimental studies on rabbits using clinical, electromyographical, and quantitative histological examinations of long autologous and homologous nerve grafts in 35 rabbits, paying special attention to quantitative histological changes in the gastrocnemius muscles, allow the statement that, compared to the short holografts, the long homografts showed worse results. Moreover, it became obvious that the regeneration rate of autografts was not influenced by increasing the lengths of the grafts. The answer to the question of how far even longer grafts may influence the quality of regeneration will need further investigations.", "contents": "Quantitative study of muscle fibre atrophy and restitution after nerve grafts. Our comparative experimental studies on rabbits using clinical, electromyographical, and quantitative histological examinations of long autologous and homologous nerve grafts in 35 rabbits, paying special attention to quantitative histological changes in the gastrocnemius muscles, allow the statement that, compared to the short holografts, the long homografts showed worse results. Moreover, it became obvious that the regeneration rate of autografts was not influenced by increasing the lengths of the grafts. The answer to the question of how far even longer grafts may influence the quality of regeneration will need further investigations.", "PMID": 961478}
{"id": "pubmed23n0032_4459", "title": "Experience with allografts in the surgery of peripheral nerves (experimental study).", "content": "Peroneal nerve allografts four to seven cm in length were transplanted in 36 tissue typed beagle dogs, using a standard microsurgical technique. The influence of tissue typing on nerve regeneration through these grafts was studied with the help of electromyography and histology seven to nine months after nerve implantation. Better regeneration was found through the grafts with compatible than with noncompatible typing. The favourable effect of tissue compatibility became more evident when the length of the graft was longer than four cm. Tissue rejection reaction was much more marked and evident with longer grafts in noncompatible than in compatible groups. Radiation, with tissue typing, did not seem to confer any additional beneficial effect. Compatible tissue typed nerve allografts probably behave more like autografts.", "contents": "Experience with allografts in the surgery of peripheral nerves (experimental study). Peroneal nerve allografts four to seven cm in length were transplanted in 36 tissue typed beagle dogs, using a standard microsurgical technique. The influence of tissue typing on nerve regeneration through these grafts was studied with the help of electromyography and histology seven to nine months after nerve implantation. Better regeneration was found through the grafts with compatible than with noncompatible typing. The favourable effect of tissue compatibility became more evident when the length of the graft was longer than four cm. Tissue rejection reaction was much more marked and evident with longer grafts in noncompatible than in compatible groups. Radiation, with tissue typing, did not seem to confer any additional beneficial effect. Compatible tissue typed nerve allografts probably behave more like autografts.", "PMID": 961479}
{"id": "pubmed23n0032_4460", "title": "Regeneration of the spinal ventral roots. An experimental study in cats.", "content": "From a clinical standpoint recovery of spinal nerve roots is less likely than that of peripheral nerves, despite their identical structures. This may be due to several factors including the length of gap to be bridged, changes in the proximal stump or neuroma, and chromatolysis. In this paper we discuss the roles of some of these factors in relation to the neuronal depopulation in the spinal cord anterior horn area following section of the anterior root. The lack of growth or the successful growth of the axon through the gap in the root influences the degree of depopulation in the anterior horn area.", "contents": "Regeneration of the spinal ventral roots. An experimental study in cats. From a clinical standpoint recovery of spinal nerve roots is less likely than that of peripheral nerves, despite their identical structures. This may be due to several factors including the length of gap to be bridged, changes in the proximal stump or neuroma, and chromatolysis. In this paper we discuss the roles of some of these factors in relation to the neuronal depopulation in the spinal cord anterior horn area following section of the anterior root. The lack of growth or the successful growth of the axon through the gap in the root influences the degree of depopulation in the anterior horn area.", "PMID": 961480}
{"id": "pubmed23n0032_4461", "title": "Radial nerve lesions and their treatment.", "content": "Out of 332 patients with peripheral nerve injuries operated upon in our Department from the beginning of 1972 to the end of 1974, in only 30 were different types of radial nerve injury observed. In most cases radial nerve palsy was seen to be a secondary occurrence following fracture of the humerus or other types of injuries. Operation revealed 16 cases of fibrosis of the radial nerve and 14 of radial nerve defect. The longest defect was 20 cm long. In the first group of patients funiculolysis was performed, and in the second group funiculorrhaphy was performed using sural nerve grafts. With the exception of one case in the first group, good reinnervation of the previously denervated muscles was achieved, as well as the restoration of sensitivity in the affected skin areas.", "contents": "Radial nerve lesions and their treatment. Out of 332 patients with peripheral nerve injuries operated upon in our Department from the beginning of 1972 to the end of 1974, in only 30 were different types of radial nerve injury observed. In most cases radial nerve palsy was seen to be a secondary occurrence following fracture of the humerus or other types of injuries. Operation revealed 16 cases of fibrosis of the radial nerve and 14 of radial nerve defect. The longest defect was 20 cm long. In the first group of patients funiculolysis was performed, and in the second group funiculorrhaphy was performed using sural nerve grafts. With the exception of one case in the first group, good reinnervation of the previously denervated muscles was achieved, as well as the restoration of sensitivity in the affected skin areas.", "PMID": 961481}
{"id": "pubmed23n0032_4462", "title": "Precise selective alcoholic Gasserian injection for tic douloureux. Recent advances in technic and results.", "content": "The Ecker-Perl method of alcoholic trigeminal rhizolysis has proved to be a safe and effective method of treating tic douloureux in a consecutive series of 324 patients over a period of 21 years.", "contents": "Precise selective alcoholic Gasserian injection for tic douloureux. Recent advances in technic and results. The Ecker-Perl method of alcoholic trigeminal rhizolysis has proved to be a safe and effective method of treating tic douloureux in a consecutive series of 324 patients over a period of 21 years.", "PMID": 961482}
{"id": "pubmed23n0032_4463", "title": "Is angiographic spasm real spasm?", "content": "Systematic morphological study of the cerebral arteries was made in six autopsy cases of ruptured aneurysms. The time course of the arterial luminal narrowing was observed by repeated angiograms, and segments of the narrowed arteries were studied histologically. Various histological changes were found consistent with the angiographic findings. We have devided these into three stages according to the duration of the disease. In the acute stage (less than one day) the contraction of the medial smooth muscle cells may be the main cause of the luminal narrowing. In the subacute stage, arteries showed a reduction in lumen size with medial thickening, marked corrugation of the internal elastic lamina, and thrombus formation attached to the endothelial surface. If vasoconstriction remained localized to the same segment for several days, the intimal or medial thickening and thrombus might produce the luminal narrowing consistent with the angiographic narrowing. In the chronic stage (more than two weeks), most cases showed dilatation of the arterial lumen on angiography. These arteries showed frank necrosis of the smooth muscle cells histologically. In a case which demonstrated progressive luminal narrowing on angiograms over 2 weeks, the arterial wall showed luminal narrowing with cellulofibrous thickening of the intima and organization of the thrombus. The presence of these structural changes in the narrowed arteries seen at angiography seems to be very important for proper understanding and treatment of vasospasm.", "contents": "Is angiographic spasm real spasm? Systematic morphological study of the cerebral arteries was made in six autopsy cases of ruptured aneurysms. The time course of the arterial luminal narrowing was observed by repeated angiograms, and segments of the narrowed arteries were studied histologically. Various histological changes were found consistent with the angiographic findings. We have devided these into three stages according to the duration of the disease. In the acute stage (less than one day) the contraction of the medial smooth muscle cells may be the main cause of the luminal narrowing. In the subacute stage, arteries showed a reduction in lumen size with medial thickening, marked corrugation of the internal elastic lamina, and thrombus formation attached to the endothelial surface. If vasoconstriction remained localized to the same segment for several days, the intimal or medial thickening and thrombus might produce the luminal narrowing consistent with the angiographic narrowing. In the chronic stage (more than two weeks), most cases showed dilatation of the arterial lumen on angiography. These arteries showed frank necrosis of the smooth muscle cells histologically. In a case which demonstrated progressive luminal narrowing on angiograms over 2 weeks, the arterial wall showed luminal narrowing with cellulofibrous thickening of the intima and organization of the thrombus. The presence of these structural changes in the narrowed arteries seen at angiography seems to be very important for proper understanding and treatment of vasospasm.", "PMID": 961483}
{"id": "pubmed23n0032_4464", "title": "Cerebral blood flow changes in acute experimental haemorrhagic vasospasm.", "content": "Subarachnoid haemorrhage was produced in 26 dogs by injecting fresh homogenous blood into the cysterna chiasmatica. Two types of vasospasm were observed, firstly segmental arterial spasm closely related to the bleeding point and secondly generalized arterial vasospasm not directly related to the bleeding point and often occurring some way from the bleeding point. Reduction in CBF occurred in 61% of cases and was always accompanied by radiological vasospasm. However, in about one quarter of the cases with vasospasm there was no alteration in CBF.", "contents": "Cerebral blood flow changes in acute experimental haemorrhagic vasospasm. Subarachnoid haemorrhage was produced in 26 dogs by injecting fresh homogenous blood into the cysterna chiasmatica. Two types of vasospasm were observed, firstly segmental arterial spasm closely related to the bleeding point and secondly generalized arterial vasospasm not directly related to the bleeding point and often occurring some way from the bleeding point. Reduction in CBF occurred in 61% of cases and was always accompanied by radiological vasospasm. However, in about one quarter of the cases with vasospasm there was no alteration in CBF.", "PMID": 961484}
{"id": "pubmed23n0032_4465", "title": "Dynamics of the blood pressure changes in sodium nitroprusside induced controlled arterial hypotension in neurosurgery.", "content": "Dynamics of the arterial blood pressure changes under the influence of intravenous infusion of sodium nitroprusside (SN) were studied in 12 patients operated on for intracranial aneurysms. Adequate measures were undertaken to avoid blood pressure (BP) changes due to the anaesthetic procedure itself. It was found that the speed of BP lowering corresponded to the rate of infusion, and could easily reach 10 mm Hg/min. After stopping the infusion, the BP recovery rate averaged a mean speed of 8.5 mm Hg/min, varying from 3.8 to 12 mm Hg/min. High speeds of BP lowering and recovery achieved with the use of SN are the main advantages of this hypotensive agent in neurosurgical procedures.", "contents": "Dynamics of the blood pressure changes in sodium nitroprusside induced controlled arterial hypotension in neurosurgery. Dynamics of the arterial blood pressure changes under the influence of intravenous infusion of sodium nitroprusside (SN) were studied in 12 patients operated on for intracranial aneurysms. Adequate measures were undertaken to avoid blood pressure (BP) changes due to the anaesthetic procedure itself. It was found that the speed of BP lowering corresponded to the rate of infusion, and could easily reach 10 mm Hg/min. After stopping the infusion, the BP recovery rate averaged a mean speed of 8.5 mm Hg/min, varying from 3.8 to 12 mm Hg/min. High speeds of BP lowering and recovery achieved with the use of SN are the main advantages of this hypotensive agent in neurosurgical procedures.", "PMID": 961485}
{"id": "pubmed23n0032_4466", "title": "The effect of increased intracranial pressure on pressure in the superior sagittal sinus.", "content": "It is difficult to explain why rises in ICP provoke different types of response in superior sagittal sinus pressure. In most of our experimental animals there was close correlation between rises in ICP and SSSP. In the remainder, SSSP showed little increase when ICP rose. The animals with marked increase in SSSP showed a greater capacity for compensation for increased amounts of intracranial fluid.", "contents": "The effect of increased intracranial pressure on pressure in the superior sagittal sinus. It is difficult to explain why rises in ICP provoke different types of response in superior sagittal sinus pressure. In most of our experimental animals there was close correlation between rises in ICP and SSSP. In the remainder, SSSP showed little increase when ICP rose. The animals with marked increase in SSSP showed a greater capacity for compensation for increased amounts of intracranial fluid.", "PMID": 961486}
{"id": "pubmed23n0032_4467", "title": "Rheographic assessment of cerebral blood volume and correlations with changes in intracranial pressure.", "content": "The authors present a four electrode rheographic technique for simiquantitative continuous measurement of CBV, by examining changes in cerebral tissue impedance induced by changes in blood content of the brain parenchyma. Correlation between the data obtained and ICP measurements permits conclusions regarding the behaviour of the cerebral blood bed. pCO2 regulates CBV, and the phenomenon is reversible according to a hystereris shaped time course in acute experiments. The rebound of ICP after sudden injections of CSF into the cisterna magna is of a vasogenic nature. Changes in CSF volume induce contrary changes in CBV. Pharmacological effects can be studied. The validity of the method is discussed.", "contents": "Rheographic assessment of cerebral blood volume and correlations with changes in intracranial pressure. The authors present a four electrode rheographic technique for simiquantitative continuous measurement of CBV, by examining changes in cerebral tissue impedance induced by changes in blood content of the brain parenchyma. Correlation between the data obtained and ICP measurements permits conclusions regarding the behaviour of the cerebral blood bed. pCO2 regulates CBV, and the phenomenon is reversible according to a hystereris shaped time course in acute experiments. The rebound of ICP after sudden injections of CSF into the cisterna magna is of a vasogenic nature. Changes in CSF volume induce contrary changes in CBV. Pharmacological effects can be studied. The validity of the method is discussed.", "PMID": 961487}
{"id": "pubmed23n0032_4468", "title": "Dynamic changes in cerebrospinal fluid pressure during neurosurgical operations.", "content": "The effects of hyperventilation, osmotic and diuretic agents (urea, frusemide), thiopentone and succinylcholine chloride on the intracranial pressure were studied in neurosurgical patients with brain tumours. We have shown that hyperventilation together with osmotic and diuretic agents is very useful for reducing increased intracranial pressure.", "contents": "Dynamic changes in cerebrospinal fluid pressure during neurosurgical operations. The effects of hyperventilation, osmotic and diuretic agents (urea, frusemide), thiopentone and succinylcholine chloride on the intracranial pressure were studied in neurosurgical patients with brain tumours. We have shown that hyperventilation together with osmotic and diuretic agents is very useful for reducing increased intracranial pressure.", "PMID": 961488}
{"id": "pubmed23n0032_4469", "title": "Continuous peroperative supraoccipital cisternal drainage.", "content": "1. The technique of continuous peroperative supraoccipital cisternal drainage for intracranial decompression by means of a slow and progressive removal of CSF from the ventricles and the basal cisterns is described. 2. This procedure was used in eight patients with good results, considerable decompression being obtained with no untoward effects. 3. In four cases of intracranial aneurysm marked intracranial hypotension was obtained, greatly facilitating treatment of the lesions. 4. In four cases of brain tumour, the reduction of intracranial hypertension was not so great, and was complemented with general antihypertensive treatment. 5. Supraoccipital cisternal drainage removes intracranial CSF, but not the fluid in the spinal theca. The tonsils of the cerebellum are thus kept floating, and this, in our experience, has prevented them from impacting in the foramen magnum and compressing the medulla,", "contents": "Continuous peroperative supraoccipital cisternal drainage. 1. The technique of continuous peroperative supraoccipital cisternal drainage for intracranial decompression by means of a slow and progressive removal of CSF from the ventricles and the basal cisterns is described. 2. This procedure was used in eight patients with good results, considerable decompression being obtained with no untoward effects. 3. In four cases of intracranial aneurysm marked intracranial hypotension was obtained, greatly facilitating treatment of the lesions. 4. In four cases of brain tumour, the reduction of intracranial hypertension was not so great, and was complemented with general antihypertensive treatment. 5. Supraoccipital cisternal drainage removes intracranial CSF, but not the fluid in the spinal theca. The tonsils of the cerebellum are thus kept floating, and this, in our experience, has prevented them from impacting in the foramen magnum and compressing the medulla,", "PMID": 961489}
{"id": "pubmed23n0032_4470", "title": "Assessment and prognosis of coma after head injury.", "content": "The Glasgow Coma Scale, based upon eye opening, verbal and motor responses has proved a practical and consistent means of monitoring the state of head injured patients. Observations made in the early stages after injury define the depth and duration of coma and, when combined with clinical features such as a patient's age and brain stem function, have been used to predict outcome. Series of cases in comparable depths of coma in Glasgow and the Netherlands showed remarkably similar outcomes at 3 months. Based upon observations made in the first 24 hours of coma after injury, data from 255 previous cases reliably predicted outcome in the majority of 92 new patients. The exceptions were patients with potential to recover who later developed complications: no patient did significantly better than predicted.", "contents": "Assessment and prognosis of coma after head injury. The Glasgow Coma Scale, based upon eye opening, verbal and motor responses has proved a practical and consistent means of monitoring the state of head injured patients. Observations made in the early stages after injury define the depth and duration of coma and, when combined with clinical features such as a patient's age and brain stem function, have been used to predict outcome. Series of cases in comparable depths of coma in Glasgow and the Netherlands showed remarkably similar outcomes at 3 months. Based upon observations made in the first 24 hours of coma after injury, data from 255 previous cases reliably predicted outcome in the majority of 92 new patients. The exceptions were patients with potential to recover who later developed complications: no patient did significantly better than predicted.", "PMID": 961490}
{"id": "pubmed23n0032_4471", "title": "Classification of coma.", "content": "By order of the Head Injuries Committee of the W.F.N.S. the author and his coworkers give definitions of consciousness, clouding of consciouness and unconciousness or coma. Furthermore, they suggest a definition of the termination of unconsciousness, with particular reference to the transition into a stage of clouding of consciousness. Any statistical evaluation and comparison of different materials depend on the use of identical definitions. As an example, it has been shown that the duration of aa uninterrupted coma, which can be survived, becomes relatively short if in accordance with the proposed definitions the various stages of clouding of consciousness are excluded from \"coma\".", "contents": "Classification of coma. By order of the Head Injuries Committee of the W.F.N.S. the author and his coworkers give definitions of consciousness, clouding of consciouness and unconciousness or coma. Furthermore, they suggest a definition of the termination of unconsciousness, with particular reference to the transition into a stage of clouding of consciousness. Any statistical evaluation and comparison of different materials depend on the use of identical definitions. As an example, it has been shown that the duration of aa uninterrupted coma, which can be survived, becomes relatively short if in accordance with the proposed definitions the various stages of clouding of consciousness are excluded from \"coma\".", "PMID": 961491}
{"id": "pubmed23n0032_4472", "title": "Assessment of the psychosocial outcome of severe head injury.", "content": "Increasing numbers of those concerned with the primary treatment and later care of individuals who sustain head injuries are becoming interested in the development of methods for assessing the outcome of severe brain damage. In the past such methods seldom involved balanced consideration of the physical, mental and social sequelae of injury. Moreover rehabilitation, which should involve restoration of patients to their fullest physical, mental and social capability, is often biased towards the improvement of physical disability alone. The long lasting and chronically disabling mental changes which occur so frequently, and which tend to cause the greatest difficulties for patients in terms of their  reintegration into society, usually receive scant attention. Assessment of outcome necessitates evaluation of the contribution of both physiogenic and psychogenic factors to the patient's mental state, and thus their respective contributions to the degree of social integration achieved. With these points in mind a pilot study was designed to evaluate three simple indices of outcome--namely neurophysical, mental and social assessment scales. The relation of each scale to the severity of brain damage, assessed in terms of post-traumatic amnesia, was examined. The relation of the scales to each other and to measures of cognitive function (the Wechsler Adult Intelligence Scale) were also considered, Results from 56 severely brain injured patients reveals a clear cut relation between the duration of post-traumatic amnesia and the measures of disability devised. Further, social disability was related to the level of physical and mental handicap, but it was the latter which caused the most severe problems. The level of intellectual recovery was related to all scales of disability and to the duration of post-traumatic amnesia, although the latter proved to be a less accurate predictor of the ultimate degree of intellectual recovery than was expected.", "contents": "Assessment of the psychosocial outcome of severe head injury. Increasing numbers of those concerned with the primary treatment and later care of individuals who sustain head injuries are becoming interested in the development of methods for assessing the outcome of severe brain damage. In the past such methods seldom involved balanced consideration of the physical, mental and social sequelae of injury. Moreover rehabilitation, which should involve restoration of patients to their fullest physical, mental and social capability, is often biased towards the improvement of physical disability alone. The long lasting and chronically disabling mental changes which occur so frequently, and which tend to cause the greatest difficulties for patients in terms of their  reintegration into society, usually receive scant attention. Assessment of outcome necessitates evaluation of the contribution of both physiogenic and psychogenic factors to the patient's mental state, and thus their respective contributions to the degree of social integration achieved. With these points in mind a pilot study was designed to evaluate three simple indices of outcome--namely neurophysical, mental and social assessment scales. The relation of each scale to the severity of brain damage, assessed in terms of post-traumatic amnesia, was examined. The relation of the scales to each other and to measures of cognitive function (the Wechsler Adult Intelligence Scale) were also considered, Results from 56 severely brain injured patients reveals a clear cut relation between the duration of post-traumatic amnesia and the measures of disability devised. Further, social disability was related to the level of physical and mental handicap, but it was the latter which caused the most severe problems. The level of intellectual recovery was related to all scales of disability and to the duration of post-traumatic amnesia, although the latter proved to be a less accurate predictor of the ultimate degree of intellectual recovery than was expected.", "PMID": 961492}
{"id": "pubmed23n0032_4473", "title": "The blink reflex in coma and after recovery from coma.", "content": "Comparative studies of the blink reflex in a series of head injuries through the stages of coma and recovery from coma in a series of miscellaneous hemispheral lesions, and in a normal series, reveals that, although the principal centre for the R1 component of the blink reflex seems to be localised in the upper pons, the seat of the R2 late component is in the reticular system. The reappearance of this late component of the blink reflex in our cases of coma although seeming to depend on the integrity of the mesencephalic formation, correlates with the recovery of the patient's alertness. On the other hand, habituation of this reflex depends on the integrity of global cognitive function, rather than on any localised centre. The roles of the cortex, selective attention, and emotional factors, are discussed. The recovery of the normal habituation of the blink reflex obtained by glabellar tapping was found to be a useful sign in the follow up of patients recovering from concussion and other lesions, such as subdural haematomas and brain tumours, with global mental impairment.", "contents": "The blink reflex in coma and after recovery from coma. Comparative studies of the blink reflex in a series of head injuries through the stages of coma and recovery from coma in a series of miscellaneous hemispheral lesions, and in a normal series, reveals that, although the principal centre for the R1 component of the blink reflex seems to be localised in the upper pons, the seat of the R2 late component is in the reticular system. The reappearance of this late component of the blink reflex in our cases of coma although seeming to depend on the integrity of the mesencephalic formation, correlates with the recovery of the patient's alertness. On the other hand, habituation of this reflex depends on the integrity of global cognitive function, rather than on any localised centre. The roles of the cortex, selective attention, and emotional factors, are discussed. The recovery of the normal habituation of the blink reflex obtained by glabellar tapping was found to be a useful sign in the follow up of patients recovering from concussion and other lesions, such as subdural haematomas and brain tumours, with global mental impairment.", "PMID": 961493}
{"id": "pubmed23n0032_4474", "title": "Initial state, outcome, and autopsy findings in a series of 200 consecutive traumatic comas. A computerised analysis.", "content": "Based on 200 nonselected consecutive cases of primary traumatic coma a preliminary mathematical model--computed, multiple linear regression analysis--has been developed, which seems to be suitable for calculating the prognosis from some initial anamnestic and clinical data. It is to be expected that the analysis of greater material will help to establish an even better model, e.g. by a more detailed age subdivision and choice of more or other factors. It is the aim of this report to encourage further work in this field.", "contents": "Initial state, outcome, and autopsy findings in a series of 200 consecutive traumatic comas. A computerised analysis. Based on 200 nonselected consecutive cases of primary traumatic coma a preliminary mathematical model--computed, multiple linear regression analysis--has been developed, which seems to be suitable for calculating the prognosis from some initial anamnestic and clinical data. It is to be expected that the analysis of greater material will help to establish an even better model, e.g. by a more detailed age subdivision and choice of more or other factors. It is the aim of this report to encourage further work in this field.", "PMID": 961494}
{"id": "pubmed23n0032_4475", "title": "Intracranial circulatory insufficiency. Surgery of carotid atheroma.", "content": "Clinical and radiological studies are presented from 81 cases of intracranial circulatory insufficiency due to extracranial atherosclerosis, and the results of surgical treatment are given.", "contents": "Intracranial circulatory insufficiency. Surgery of carotid atheroma. Clinical and radiological studies are presented from 81 cases of intracranial circulatory insufficiency due to extracranial atherosclerosis, and the results of surgical treatment are given.", "PMID": 961495}
{"id": "pubmed23n0032_4476", "title": "New approach to brain tumour chemoradiotherapy with cellular synchronization by colcemid.", "content": "Cellular synchronization using Colcemid as pretreatment for combined chemoradiotherapy was investigated. C6 rat brain tumour was cultured in RPMI medium containing 10(-5)-10(-7) Mol. of Colcemid for 24 hours. The basic cell kinetics were analysed with a Pulse Cytophotometer, which facilitated the analysis of tumour cell cycle phase distribution according to the DNA content. The effect of Colcemid depended on the concentration, and the minimal concentration showing continuous blocking during 48 hours after removal of the drug was 10(-6) Mol. G1 fraction of 2 C DNA content was reduced from 74% to 36%. G2-M phase of 4 C DNA content increased from 9% to 28%. S phase cells increased from 17% to 31%. Polyploid cells in the Tetraploid cell cycle could be recognized. The remaining 36% of cells within the GO + G1 peak of 2 C DNA content were considered to be diploid GO cells.", "contents": "New approach to brain tumour chemoradiotherapy with cellular synchronization by colcemid. Cellular synchronization using Colcemid as pretreatment for combined chemoradiotherapy was investigated. C6 rat brain tumour was cultured in RPMI medium containing 10(-5)-10(-7) Mol. of Colcemid for 24 hours. The basic cell kinetics were analysed with a Pulse Cytophotometer, which facilitated the analysis of tumour cell cycle phase distribution according to the DNA content. The effect of Colcemid depended on the concentration, and the minimal concentration showing continuous blocking during 48 hours after removal of the drug was 10(-6) Mol. G1 fraction of 2 C DNA content was reduced from 74% to 36%. G2-M phase of 4 C DNA content increased from 9% to 28%. S phase cells increased from 17% to 31%. Polyploid cells in the Tetraploid cell cycle could be recognized. The remaining 36% of cells within the GO + G1 peak of 2 C DNA content were considered to be diploid GO cells.", "PMID": 961496}
{"id": "pubmed23n0032_4477", "title": "Mathematical analysis (fast Fourier transform) of the ultrasonic echo pulsation curve in brain diseases.", "content": "The ultrasonic pulsation curve can be analysed by the computer. This technique has been useful for measuring haemodynamics. We detected prodromal signs of rebleeding from an intracranial aneurysm.", "contents": "Mathematical analysis (fast Fourier transform) of the ultrasonic echo pulsation curve in brain diseases. The ultrasonic pulsation curve can be analysed by the computer. This technique has been useful for measuring haemodynamics. We detected prodromal signs of rebleeding from an intracranial aneurysm.", "PMID": 961497}
{"id": "pubmed23n0032_4478", "title": "Recovery from experimental paraplegia after levodopa administration.", "content": "In decompression sickness and during some surgical procedures, air emboli that form sometimes cause serious damage if the gas bubbles find their way to the vital organs. Paralysis of the spinal cord is one of the most serious manifestations induced by air emboli. Exposure to compression chambers is effective in air emboli treatment, but availability of chambers is inadequate and the treatment is lengthy. Until now there has been no fully effective injectable agent that can remedy the damage caused by air embolization. In this work levodopa was chosen as an injectable drug that might help to improve recovery from experimental paraplegia because of the reported effects of levodopa on muscle tone, spasticity and locomotion. To induce air emboli, the descending aorta of rats was chronically cannulated. Two weeks later, after full recovery from surgery, air was injected through the chronically implanted cannula into unanesthetized rats (0.35 ml of air per 100 g, during 4 sec). The paraplegia (paralysis of both hind legs) was manifested 2-10 minutes later. Only animals that had total paraplegia, without any sensation, were used in the experiments. Levodopa was administered 2 minutes after paraplegia was established. The levodopa treatment was repeated each day during one week. After six days, ten levodopa treated (intra-arterially) animals in a group of twelve and six levodopa treated (intraperitoneally) animals in a group of eight recovered completely from paraplegia. In control groups only three from thirteen (untreated), or two from twelve (solvent administration) animals recovered from paraplegia.", "contents": "Recovery from experimental paraplegia after levodopa administration. In decompression sickness and during some surgical procedures, air emboli that form sometimes cause serious damage if the gas bubbles find their way to the vital organs. Paralysis of the spinal cord is one of the most serious manifestations induced by air emboli. Exposure to compression chambers is effective in air emboli treatment, but availability of chambers is inadequate and the treatment is lengthy. Until now there has been no fully effective injectable agent that can remedy the damage caused by air embolization. In this work levodopa was chosen as an injectable drug that might help to improve recovery from experimental paraplegia because of the reported effects of levodopa on muscle tone, spasticity and locomotion. To induce air emboli, the descending aorta of rats was chronically cannulated. Two weeks later, after full recovery from surgery, air was injected through the chronically implanted cannula into unanesthetized rats (0.35 ml of air per 100 g, during 4 sec). The paraplegia (paralysis of both hind legs) was manifested 2-10 minutes later. Only animals that had total paraplegia, without any sensation, were used in the experiments. Levodopa was administered 2 minutes after paraplegia was established. The levodopa treatment was repeated each day during one week. After six days, ten levodopa treated (intra-arterially) animals in a group of twelve and six levodopa treated (intraperitoneally) animals in a group of eight recovered completely from paraplegia. In control groups only three from thirteen (untreated), or two from twelve (solvent administration) animals recovered from paraplegia.", "PMID": 961498}
{"id": "pubmed23n0032_4479", "title": "Molecular evidence for a viral etiology of human CNS tumors.", "content": "The newer methods of molecular virology, including molecular hybridization and the \"simultaneous detection test,\" were used to examine human brain tumors for evidence of RNA tumor viruses. It was found that they contained 70S RNA and RNA-directed DNA polymerase, both encapsulated in a particle possessing a density of 1.17 g/ml. These particles therefore satisfy the three diagnostic features that characterize the animal RNA tumor viruses. Of 26 of the most malignant (glioblastoma and medullo blastoma) brain tumors examined, 24 (92%) contained these virus-like entities. The possible usefulness of these particles as aids in diagnosis and monitoring therapy is briefly discussed.", "contents": "Molecular evidence for a viral etiology of human CNS tumors. The newer methods of molecular virology, including molecular hybridization and the \"simultaneous detection test,\" were used to examine human brain tumors for evidence of RNA tumor viruses. It was found that they contained 70S RNA and RNA-directed DNA polymerase, both encapsulated in a particle possessing a density of 1.17 g/ml. These particles therefore satisfy the three diagnostic features that characterize the animal RNA tumor viruses. Of 26 of the most malignant (glioblastoma and medullo blastoma) brain tumors examined, 24 (92%) contained these virus-like entities. The possible usefulness of these particles as aids in diagnosis and monitoring therapy is briefly discussed.", "PMID": 961499}
{"id": "pubmed23n0032_4480", "title": "Monoamine acid metabolites in ventricular CSF of patients with brain tumours.", "content": "Homovanillic acid (HVA) and 5-hydroxyindolacetic acid (5-HIAA) in ventricular CSF were determined in 19 patients (12 female 7 male) with brain tumours. No relationship was found between ventricular fluid pressure (VFP) and levels of HVA and 5-HIAA. A relationship was observed between ventricular CSF, HVA concentrations and tumour induced alterations in CSF dynamics. HVA concentrations were very high in patients whose tumours involved the third ventricle, the aqueduct, or the fourth ventricle, producing marked alterations in CSF flow. HVA concentrations significantly lower than in controls were observed in cases where tumours involved the lateral ventricles. Concentrations of acid metabolites in patients with little or no alteration in CSF dynamics corresponded with those in patients with other neurosurgical disorders.", "contents": "Monoamine acid metabolites in ventricular CSF of patients with brain tumours. Homovanillic acid (HVA) and 5-hydroxyindolacetic acid (5-HIAA) in ventricular CSF were determined in 19 patients (12 female 7 male) with brain tumours. No relationship was found between ventricular fluid pressure (VFP) and levels of HVA and 5-HIAA. A relationship was observed between ventricular CSF, HVA concentrations and tumour induced alterations in CSF dynamics. HVA concentrations were very high in patients whose tumours involved the third ventricle, the aqueduct, or the fourth ventricle, producing marked alterations in CSF flow. HVA concentrations significantly lower than in controls were observed in cases where tumours involved the lateral ventricles. Concentrations of acid metabolites in patients with little or no alteration in CSF dynamics corresponded with those in patients with other neurosurgical disorders.", "PMID": 961500}
{"id": "pubmed23n0032_4481", "title": "Microsurgical lumbosacral ganglionectomy, anatomic rationale, and surgical results.", "content": "Recent anatomic and physiologic work suggests that there may be substantial numbers of unmyelinated sensory axons in the ventral roots. The cell bodies of origin for these unmyelinated axons would presumably be located in the dorsal ganglion. We have performed 18 microsurgical lumbosacral ganglionectomies over the past two years for chronic lumbosacral pain syndromes. In spite of preoperative nerve block trials, good results were obtained in only 10 of 18 patients. Four patients obtained some pain relief, and four patients no pain relief whatsoever. Microganglionectomy in the lumbosacral area is probably easier technically than the more traditional intradural rhizotomy, but may not yield any better results.", "contents": "Microsurgical lumbosacral ganglionectomy, anatomic rationale, and surgical results. Recent anatomic and physiologic work suggests that there may be substantial numbers of unmyelinated sensory axons in the ventral roots. The cell bodies of origin for these unmyelinated axons would presumably be located in the dorsal ganglion. We have performed 18 microsurgical lumbosacral ganglionectomies over the past two years for chronic lumbosacral pain syndromes. In spite of preoperative nerve block trials, good results were obtained in only 10 of 18 patients. Four patients obtained some pain relief, and four patients no pain relief whatsoever. Microganglionectomy in the lumbosacral area is probably easier technically than the more traditional intradural rhizotomy, but may not yield any better results.", "PMID": 961501}
{"id": "pubmed23n0032_4482", "title": "Changes in serum and cerebrospinal fluid enzyme activity after head injury.", "content": "We studied simultaneously in serum (S) and CSF (L) the enzyme activities of GOT, GPT, LDH, ICDH, MDH, ALD, and CPK in 28 patients with head injuries divided into three groups according to the severity of the trauma. We found a correlation between severity of brain lesion and enzyme activity. The best correlation was found for SGOT, SCPK, LGOT, LLDH, LMDH and LCPK. We do not believe that enzyme activity is of prognostic value. We think that further studies should be made of the specific isoenzymes of the Central Nervous System.", "contents": "Changes in serum and cerebrospinal fluid enzyme activity after head injury. We studied simultaneously in serum (S) and CSF (L) the enzyme activities of GOT, GPT, LDH, ICDH, MDH, ALD, and CPK in 28 patients with head injuries divided into three groups according to the severity of the trauma. We found a correlation between severity of brain lesion and enzyme activity. The best correlation was found for SGOT, SCPK, LGOT, LLDH, LMDH and LCPK. We do not believe that enzyme activity is of prognostic value. We think that further studies should be made of the specific isoenzymes of the Central Nervous System.", "PMID": 961502}
{"id": "pubmed23n0032_4483", "title": "Cytotoxic antibodies in preoperative glioma patients: a diagnostic assay.", "content": "The use of a microcytotoxicity test for complement-dependent serum antibodies cytotoxic to cultured astrocytoma cells has been explored as an aid in the diagnosis of brain tumors. Fourteen of 21 randomly selected preoperative glioma patients (67%) had antibodies detectable by this assay in contrast to 4 of 21 normal blood donors (19%). Five of the glioma patients (24%) were strongly positive (cytotoxic index greater than 80-80) while none of the controls was in this range. Although positive responses were seen in patients with tumors of every grade, only 6 of 11 patients with astrocytomas of Grades I-III were positive as compared to 8 of 9 patients with Grade IV astrocytomas. The microcytotoxicity test used is simple, inexpensive and capable of being performed on an outpatient basis. It is concluded that this assay has usefulness both as a screening technique and as a diagnostic adjunct for patients suspected of having brain tumors.", "contents": "Cytotoxic antibodies in preoperative glioma patients: a diagnostic assay. The use of a microcytotoxicity test for complement-dependent serum antibodies cytotoxic to cultured astrocytoma cells has been explored as an aid in the diagnosis of brain tumors. Fourteen of 21 randomly selected preoperative glioma patients (67%) had antibodies detectable by this assay in contrast to 4 of 21 normal blood donors (19%). Five of the glioma patients (24%) were strongly positive (cytotoxic index greater than 80-80) while none of the controls was in this range. Although positive responses were seen in patients with tumors of every grade, only 6 of 11 patients with astrocytomas of Grades I-III were positive as compared to 8 of 9 patients with Grade IV astrocytomas. The microcytotoxicity test used is simple, inexpensive and capable of being performed on an outpatient basis. It is concluded that this assay has usefulness both as a screening technique and as a diagnostic adjunct for patients suspected of having brain tumors.", "PMID": 961503}
{"id": "pubmed23n0032_4484", "title": "Heterologous antibodies in human and rat's glioblastoma.", "content": "IgG from immunized rabbits was labelled with Fluorescein Isothiocyanate, and conjugated in vitro with human and with rat glioblastoma. After absorption of the antisera with normal brain and liver, a heavy concentration of antibody was found in the rat tumour cells. A great amount of antibody was also found in the human neoplastic cells. The incorporation of this antibody in glioma cells of different rats developing new tumours indicates that transplantation antigens of the H-2 type are not the only ones capable of inducing specific antibody attachment.", "contents": "Heterologous antibodies in human and rat's glioblastoma. IgG from immunized rabbits was labelled with Fluorescein Isothiocyanate, and conjugated in vitro with human and with rat glioblastoma. After absorption of the antisera with normal brain and liver, a heavy concentration of antibody was found in the rat tumour cells. A great amount of antibody was also found in the human neoplastic cells. The incorporation of this antibody in glioma cells of different rats developing new tumours indicates that transplantation antigens of the H-2 type are not the only ones capable of inducing specific antibody attachment.", "PMID": 961504}
{"id": "pubmed23n0032_4485", "title": "Change of local spinal cord blood flow in acute ischaemia.", "content": "The local blood flow of the spinal cord was recorded by the hydrogen polarographic method with platinum electrodes. The blood flow and its changes were measured during acute ischaemia caused by compression of the thoracic aorta, and also after release of compression. Ten cats were included in these experiments, and more than 100 measurements were made in the lumbar region of the spinal cord. In the grey matter a high flow was observed in every case, resembling that in the cerebral cortex. Reduction of the lumen of the thoracic aorta by one half resulted in a 23%, and by three quarters in a 60-70% decrease in blood flow. With total occlusion there was practically entire cessation of flow. Rapid restitution of blood supply in the grey matter of the spinal cord caused a 65%, and in the white matter a 170% increase in blood flow due to reactive hyperaemia. Normal flow rates returned within 2-2.5 minutes.", "contents": "Change of local spinal cord blood flow in acute ischaemia. The local blood flow of the spinal cord was recorded by the hydrogen polarographic method with platinum electrodes. The blood flow and its changes were measured during acute ischaemia caused by compression of the thoracic aorta, and also after release of compression. Ten cats were included in these experiments, and more than 100 measurements were made in the lumbar region of the spinal cord. In the grey matter a high flow was observed in every case, resembling that in the cerebral cortex. Reduction of the lumen of the thoracic aorta by one half resulted in a 23%, and by three quarters in a 60-70% decrease in blood flow. With total occlusion there was practically entire cessation of flow. Rapid restitution of blood supply in the grey matter of the spinal cord caused a 65%, and in the white matter a 170% increase in blood flow due to reactive hyperaemia. Normal flow rates returned within 2-2.5 minutes.", "PMID": 961505}
{"id": "pubmed23n0032_4486", "title": "A long term observation of local cerebral blood flow using the hydrogen gas clearance technique.", "content": "Local blood flow was estimated in the cerebral hemispheres of conscious goats using implanted platinum electrodes and the hydrogen gas clearance technique over periods of several weeks. During the first days of the experiments the blood flow rate was either below 27 ml/100 g/min. or above 40 ml/100 g/min. A gradual decrease in flow was observed over five weeks. The final flow values were between 20 ml/100 g/min. and 30 ml/100 g/min. Carotid injections of serotonin, noradrenalin, or metaoxamine, and inhalation of 5% carbon dioxide produced only insignificant changes in local blood flow. The cause of the slow decrease of flow and the change from a biexponential to a monoexponential curve may be explained by local tissue changes during the observation periods.", "contents": "A long term observation of local cerebral blood flow using the hydrogen gas clearance technique. Local blood flow was estimated in the cerebral hemispheres of conscious goats using implanted platinum electrodes and the hydrogen gas clearance technique over periods of several weeks. During the first days of the experiments the blood flow rate was either below 27 ml/100 g/min. or above 40 ml/100 g/min. A gradual decrease in flow was observed over five weeks. The final flow values were between 20 ml/100 g/min. and 30 ml/100 g/min. Carotid injections of serotonin, noradrenalin, or metaoxamine, and inhalation of 5% carbon dioxide produced only insignificant changes in local blood flow. The cause of the slow decrease of flow and the change from a biexponential to a monoexponential curve may be explained by local tissue changes during the observation periods.", "PMID": 961506}
{"id": "pubmed23n0032_4487", "title": "Ultrastructural findings after microsurgical interventions on the carotid artery of the rat.", "content": "For 10 years, it has been known that operations on vessels 1 mm in diameter are possible. The application of microvascular techniques to neurosurgery demands microscopic and ultrastructural examinations of the effects of such interventions on small vessels. Histological examinations can help to provide answers to questions concerning operating technique, ultrastructural examinations give information on the indications for operation. Since these questions have not been studied previously, preliminary examinations on easily accessible vessels are necessary. For this purpose, the common carotid artery of the rat was chosen. Histological and ultrastructural examinations were carried out on end-to-end anastomoses of these vessels. The ultrastructural findings are described and compared with anatomical findings in normal and abnormal vessels in the rat.", "contents": "Ultrastructural findings after microsurgical interventions on the carotid artery of the rat. For 10 years, it has been known that operations on vessels 1 mm in diameter are possible. The application of microvascular techniques to neurosurgery demands microscopic and ultrastructural examinations of the effects of such interventions on small vessels. Histological examinations can help to provide answers to questions concerning operating technique, ultrastructural examinations give information on the indications for operation. Since these questions have not been studied previously, preliminary examinations on easily accessible vessels are necessary. For this purpose, the common carotid artery of the rat was chosen. Histological and ultrastructural examinations were carried out on end-to-end anastomoses of these vessels. The ultrastructural findings are described and compared with anatomical findings in normal and abnormal vessels in the rat.", "PMID": 961507}
{"id": "pubmed23n0032_4488", "title": "Experimental middle cerebral artery microsurgical embolectomy.", "content": "The canine middle cerebral has been embolized using the method of Molinari. Microsurgical embolectomies were done at two hours and six hours post-embolism. Animals done at two hours remained virtually intact neurologically, and hence fared better than control dogs whose embolus remained in place. Animals done six hours post embolism had increased neurologic morbidity and mortality as compared to the controls. Pathologic study revealed hemorrhagic infarctions in 50% of the animals done six hours post embolism.", "contents": "Experimental middle cerebral artery microsurgical embolectomy. The canine middle cerebral has been embolized using the method of Molinari. Microsurgical embolectomies were done at two hours and six hours post-embolism. Animals done at two hours remained virtually intact neurologically, and hence fared better than control dogs whose embolus remained in place. Animals done six hours post embolism had increased neurologic morbidity and mortality as compared to the controls. Pathologic study revealed hemorrhagic infarctions in 50% of the animals done six hours post embolism.", "PMID": 961508}
{"id": "pubmed23n0032_4489", "title": "Some observations on the microneurosurgical treatment of intracranial aneurysms.", "content": "This paper reports a retrospective study of the microneurosurgical management of intracranial aneurysm in 133 patients. Good or fair results were obtained in 76%, 12% of patients had a poor result and the mortality was 12%. Major factors which were found to influence the outcome of surgery were: pre-operatively, the Botterell grade of the patient, pre-existing systemic hypertension and the time interval between the last subarachnoid haemorrhage and surgery. Post-operatively, the development of cerebral vasospasm was associated with a poor outcome from surgery. Better results might be obtained from the surgery of intracranial aneurysm by delaying operation to the second week after subarachnoid haemorrhage and by better management of hypertensive patients pre-operatively and patients who develop cerebral vasospasm post-operatively.", "contents": "Some observations on the microneurosurgical treatment of intracranial aneurysms. This paper reports a retrospective study of the microneurosurgical management of intracranial aneurysm in 133 patients. Good or fair results were obtained in 76%, 12% of patients had a poor result and the mortality was 12%. Major factors which were found to influence the outcome of surgery were: pre-operatively, the Botterell grade of the patient, pre-existing systemic hypertension and the time interval between the last subarachnoid haemorrhage and surgery. Post-operatively, the development of cerebral vasospasm was associated with a poor outcome from surgery. Better results might be obtained from the surgery of intracranial aneurysm by delaying operation to the second week after subarachnoid haemorrhage and by better management of hypertensive patients pre-operatively and patients who develop cerebral vasospasm post-operatively.", "PMID": 961509}
{"id": "pubmed23n0032_4490", "title": "The role of cytochrome P-450 in the regulation of steroid biosynthesis.", "content": "A cytochrome P-450 from bovine adrenocortical mitochondria has been purified to near homogeneity. The protein catalyzes side-chain cleavage of cholesterol (cholesterol leads to pregnenolone) but neither 11beta- nor 18-hydroxylation. It consists of 16 subunits of two species (MW 52,000) and contains 8 heme groups. The enzyme has been used to determine the stoichiometry of side-chain cleavage with the following results: (TPNH and O2 consumed/mole of cleavage), cholesterol 3:3:1, 20S-hydroxycholesterol 2:2:1 and 20S,22R-dihydroxycholesterol 1:1:1. These findings support the occurrence of the proposed pathway for the side-chain cleavage of cholesterol. Cleavage of the diol is inhibited by CO and shows a characteristic P-450 photochemical action spectrum. Evidently the diol is cleaved in a typical monoxygenase reaction. The active form of the enzyme contains 16 subunits (protein 16); forms consisting of 8 (protein 8) and 4 (protein 4) subunits can be isolated and are enzymatically active only by prior conversion to protein 16.", "contents": "The role of cytochrome P-450 in the regulation of steroid biosynthesis. A cytochrome P-450 from bovine adrenocortical mitochondria has been purified to near homogeneity. The protein catalyzes side-chain cleavage of cholesterol (cholesterol leads to pregnenolone) but neither 11beta- nor 18-hydroxylation. It consists of 16 subunits of two species (MW 52,000) and contains 8 heme groups. The enzyme has been used to determine the stoichiometry of side-chain cleavage with the following results: (TPNH and O2 consumed/mole of cleavage), cholesterol 3:3:1, 20S-hydroxycholesterol 2:2:1 and 20S,22R-dihydroxycholesterol 1:1:1. These findings support the occurrence of the proposed pathway for the side-chain cleavage of cholesterol. Cleavage of the diol is inhibited by CO and shows a characteristic P-450 photochemical action spectrum. Evidently the diol is cleaved in a typical monoxygenase reaction. The active form of the enzyme contains 16 subunits (protein 16); forms consisting of 8 (protein 8) and 4 (protein 4) subunits can be isolated and are enzymatically active only by prior conversion to protein 16.", "PMID": 961534}
{"id": "pubmed23n0032_4491", "title": "Contractile effects of histamine in large and small respiratory airways.", "content": "The described technique allows recording of circular smooth muscle activity in isolated airways approx. 1 mm in diameter (bronchioles). It also allows recording of graded contractile and relaxant responses to drugs. Bronchiolar preparations and spirally cut airways (diameters 3-6 mm) were obtained from cat, dog, guinea-pig and man. Histamine is shown to contract cat bronchioles, large airways in cat being unaffected. The contraction is blocked by brompheniramine, but not by atropine. Other contractile agents, cholinoceptor-stimulants, prostaglandin F2alpha'5-hydroxy-tryptamine, and potassium contract the isolated cat airways irrespective of their size. In preparations from dog, guinea-pig and man,histamine was shown to contract both small and large respiratory airways. The effect was blocked by brompheniramine, which did not change the effect of acetylcholine or pilocarpine. Both large and small airways contracted to cholinoceptor stimulation Within species, small and large airways were similarly sensitive to the contractile agents, except for histamine in cat airways. The present findings show a size-and species dependent effect of histamine in respiratory airways. The effect of histamine in isolated cat airways might partly explain the pulmonary effect of histamine in vivo. The importance of including both small and large airways in studies of contractile and relaxant effects is emphasized.", "contents": "Contractile effects of histamine in large and small respiratory airways. The described technique allows recording of circular smooth muscle activity in isolated airways approx. 1 mm in diameter (bronchioles). It also allows recording of graded contractile and relaxant responses to drugs. Bronchiolar preparations and spirally cut airways (diameters 3-6 mm) were obtained from cat, dog, guinea-pig and man. Histamine is shown to contract cat bronchioles, large airways in cat being unaffected. The contraction is blocked by brompheniramine, but not by atropine. Other contractile agents, cholinoceptor-stimulants, prostaglandin F2alpha'5-hydroxy-tryptamine, and potassium contract the isolated cat airways irrespective of their size. In preparations from dog, guinea-pig and man,histamine was shown to contract both small and large respiratory airways. The effect was blocked by brompheniramine, which did not change the effect of acetylcholine or pilocarpine. Both large and small airways contracted to cholinoceptor stimulation Within species, small and large airways were similarly sensitive to the contractile agents, except for histamine in cat airways. The present findings show a size-and species dependent effect of histamine in respiratory airways. The effect of histamine in isolated cat airways might partly explain the pulmonary effect of histamine in vivo. The importance of including both small and large airways in studies of contractile and relaxant effects is emphasized.", "PMID": 961543}
{"id": "pubmed23n0032_4492", "title": "An investigation of the therapeutic value of the 'copper bracelet'-dermal assimilation of copper in arthritic/rheumatoid conditions.", "content": "From over 300 arthritis sufferers, half of whom previously wore 'copper bracelets', three treatment-group-subjects were randomly allocated for a psychological study. This involved wearing 'copper bracelets' and placebo bracelets (amodised aluminum resembling copper) alternately. These groups, as also a control group, answered questionnaires. The copper bracelets were weighed before and after use. Preliminary results show that, to a significant number of subjects, the wearing of the 'copper bracelet' appeared to have some therapeutic value. A study of the components of sweat and the solubility of copper in sweat was carried out. In five sweat samples, the copper concentration was of the order 2 x 10(-5) M and after equilibrating with copper turnings at room temperature for 24 hours the samples turned blue in colour and contained approximately 2 x 10(-3) M copper. The permeability of skin to copper containing solutions has also been discussed. It has also been shown that the wearing of a 'copper bracelet' results in a weight loss in excess of the body's total burden of copper (100-150 mg). A pair of copper bracelets lost 80 mg in 50 days when worn around the ankles and a copper bracelet worn around the wrist also lost about 90 mg in that time.", "contents": "An investigation of the therapeutic value of the 'copper bracelet'-dermal assimilation of copper in arthritic/rheumatoid conditions. From over 300 arthritis sufferers, half of whom previously wore 'copper bracelets', three treatment-group-subjects were randomly allocated for a psychological study. This involved wearing 'copper bracelets' and placebo bracelets (amodised aluminum resembling copper) alternately. These groups, as also a control group, answered questionnaires. The copper bracelets were weighed before and after use. Preliminary results show that, to a significant number of subjects, the wearing of the 'copper bracelet' appeared to have some therapeutic value. A study of the components of sweat and the solubility of copper in sweat was carried out. In five sweat samples, the copper concentration was of the order 2 x 10(-5) M and after equilibrating with copper turnings at room temperature for 24 hours the samples turned blue in colour and contained approximately 2 x 10(-3) M copper. The permeability of skin to copper containing solutions has also been discussed. It has also been shown that the wearing of a 'copper bracelet' results in a weight loss in excess of the body's total burden of copper (100-150 mg). A pair of copper bracelets lost 80 mg in 50 days when worn around the ankles and a copper bracelet worn around the wrist also lost about 90 mg in that time.", "PMID": 961545}
{"id": "pubmed23n0032_4493", "title": "Protection by phalloidin against lethal doses of phalloidin.", "content": "Tolerated doses of phalloidin, a toxin from the mushroom Amanita phalloides, protect mice against lethal doses of phalloidin. Resistance is conferred by the 1/10 LD95 of phalloidin and sets in at about 8 hours after the pretreatment.", "contents": "Protection by phalloidin against lethal doses of phalloidin. Tolerated doses of phalloidin, a toxin from the mushroom Amanita phalloides, protect mice against lethal doses of phalloidin. Resistance is conferred by the 1/10 LD95 of phalloidin and sets in at about 8 hours after the pretreatment.", "PMID": 961546}
{"id": "pubmed23n0032_4494", "title": "Inactivation of bradykinin the plumonary circulation.", "content": "Bradykinin is very efficiently inactivated on passage through the pulmonary circulation by enzymes on the vascular walls. Several different cleavages of the bradykinin molecule have been observed; one appears to be due to angiotensin converting enzyme. Several types of inhibitors have been useful in the study of these pulmonary peptidases and have helped increase understanding of the functioning of the angiotensin and plasma kinin systems.", "contents": "Inactivation of bradykinin the plumonary circulation. Bradykinin is very efficiently inactivated on passage through the pulmonary circulation by enzymes on the vascular walls. Several different cleavages of the bradykinin molecule have been observed; one appears to be due to angiotensin converting enzyme. Several types of inhibitors have been useful in the study of these pulmonary peptidases and have helped increase understanding of the functioning of the angiotensin and plasma kinin systems.", "PMID": 961547}
{"id": "pubmed23n0032_4495", "title": "The emotional aftermath of adoption.", "content": "Adopted children are emotionally vulnerable. Adoptive parents must cope with more complex problems than biologic parents. The family physician can provide valuable counseling. Preadoption counseling focuses on motivation and ambivalence. After adoption, however, serious, sometimes predictable, issues arise, such as: how and when to tell the child he is adopted; the child's search for knowledge; the problem of subsequent divorce; the birth of a natural sibling, and the involvement of other family members. New concepts include \"open adoption\" and \"single parent adoption.\"", "contents": "The emotional aftermath of adoption. Adopted children are emotionally vulnerable. Adoptive parents must cope with more complex problems than biologic parents. The family physician can provide valuable counseling. Preadoption counseling focuses on motivation and ambivalence. After adoption, however, serious, sometimes predictable, issues arise, such as: how and when to tell the child he is adopted; the child's search for knowledge; the problem of subsequent divorce; the birth of a natural sibling, and the involvement of other family members. New concepts include \"open adoption\" and \"single parent adoption.\"", "PMID": 961560}
{"id": "pubmed23n0032_4496", "title": "Influenza: current concepts.", "content": "Changes in surface antigens make preparation of an effective vaccine against the influenza virus a difficult job. When the changes are small (antigenic drift), a vaccine can be expected to be of some value; when larger changes occur (antigenic shift), its effectiveness is greatly curtailed. Improvements in the purification process for the vaccine have greatly lessened the incidence and severity of reactions. Although the current vaccines are still imperfect, they represent the best available control.", "contents": "Influenza: current concepts. Changes in surface antigens make preparation of an effective vaccine against the influenza virus a difficult job. When the changes are small (antigenic drift), a vaccine can be expected to be of some value; when larger changes occur (antigenic shift), its effectiveness is greatly curtailed. Improvements in the purification process for the vaccine have greatly lessened the incidence and severity of reactions. Although the current vaccines are still imperfect, they represent the best available control.", "PMID": 961563}
{"id": "pubmed23n0032_4497", "title": "Management of ocular foreign bodies.", "content": "Fluorescein staining is helpful in diagnosis. Many conjunctival foreign bodies can be removed by swab or irrigation, without anesthesia. Cycloplegics can prevent painful ciliary spasm. A surgical opening may be required for deeply embedded objects. Topical anesthesia is required for corneal foreign body removal. Caution: x-rays must be made to rule out intraocular foreign bodies in cases of flying metal. Rust rings must be removed. Mydriatics or cycloplegics can precipitate glaucoma. Corneal abrasions are easily infected. Secondary iritis may follow deeply embedded foreign bodies.", "contents": "Management of ocular foreign bodies. Fluorescein staining is helpful in diagnosis. Many conjunctival foreign bodies can be removed by swab or irrigation, without anesthesia. Cycloplegics can prevent painful ciliary spasm. A surgical opening may be required for deeply embedded objects. Topical anesthesia is required for corneal foreign body removal. Caution: x-rays must be made to rule out intraocular foreign bodies in cases of flying metal. Rust rings must be removed. Mydriatics or cycloplegics can precipitate glaucoma. Corneal abrasions are easily infected. Secondary iritis may follow deeply embedded foreign bodies.", "PMID": 961564}
{"id": "pubmed23n0032_4498", "title": "Diabetic osteopathy of the foot and ankle.", "content": "Neuropathic osteopathy of the foot and ankle is almost always due to underlying diabetes mellitus. The \"Charcot joint,\" or destructive type, affects the ankle or tarsal area, and the \"bone absorption,\" or mutilating type, usually affects the forefoot. If both types are present in the feet, it is almost certain that the patient is a diabetic. While these findings are usually seen in patients with long-standing, poorly controlled diabetes, they may be the first indication of diabetes.", "contents": "Diabetic osteopathy of the foot and ankle. Neuropathic osteopathy of the foot and ankle is almost always due to underlying diabetes mellitus. The \"Charcot joint,\" or destructive type, affects the ankle or tarsal area, and the \"bone absorption,\" or mutilating type, usually affects the forefoot. If both types are present in the feet, it is almost certain that the patient is a diabetic. While these findings are usually seen in patients with long-standing, poorly controlled diabetes, they may be the first indication of diabetes.", "PMID": 961565}
{"id": "pubmed23n0032_4499", "title": "Caring for the hypochondriac.", "content": "The patient's manner and the content of his complaints offer early diagnostic leads. The hypochondriac clings to his symptoms. Rather than relief, his goal is forming a relationship with the doctor that provides care and understanding. The physician's goal must be the provision of this care in such manner as to reassure, treat sparingly and convert this potentially fatal illness to a chronic disorder with which the patient can function to the limits of his capabilities.", "contents": "Caring for the hypochondriac. The patient's manner and the content of his complaints offer early diagnostic leads. The hypochondriac clings to his symptoms. Rather than relief, his goal is forming a relationship with the doctor that provides care and understanding. The physician's goal must be the provision of this care in such manner as to reassure, treat sparingly and convert this potentially fatal illness to a chronic disorder with which the patient can function to the limits of his capabilities.", "PMID": 961566}
{"id": "pubmed23n0032_4500", "title": "Viral coronary arteritis and myocardial infarction.", "content": "Two young male patients are described in whom a viral type of upper respiratory tract infection was followed by myocardial infarction. Based upon previously reported experimental studies in mice and monkeys in which Coxsackie B4 virus produced extensive coronary arterial and capillary injury, and upon the clinical data in these two patients, it is suggested that the myocardial infarcts in our two patients were the result of viral coronary arteritis.", "contents": "Viral coronary arteritis and myocardial infarction. Two young male patients are described in whom a viral type of upper respiratory tract infection was followed by myocardial infarction. Based upon previously reported experimental studies in mice and monkeys in which Coxsackie B4 virus produced extensive coronary arterial and capillary injury, and upon the clinical data in these two patients, it is suggested that the myocardial infarcts in our two patients were the result of viral coronary arteritis.", "PMID": 961568}
{"id": "pubmed23n0032_4501", "title": "A comparative study of 48 host valve and 24 prosthetic valve endocarditis cases.", "content": "Forty-eight cases of host valve endocarditis and 24 cases of prosthetic valve endocarditis occurring during a concurrent period of time were analyzed to assess differences between the two groups. The over-all incidence of prosthetic valve endocarditis during this 40 month period was 4.4%. The symptoms and signs in both groups were similar, except that PVE patients had more frequent occurrences of changing heart murmurs and splenic and cerebral emboli. Spleen scans may be helpful in the diagnosis of selected cases of culture-negative prosthetic valve endocarditis. There was no significant difference between the two groups for the various infecting microorganisms. However, the culture-negative prosthetic valve group had a mortality rate of 77.7% compared to 46.2% for the host valve group. In the HVE patients the oral cavity or urinary tract was the probable source of infection in 50.0% of the patients. In about one third of HVE cases, there was strong evidence that the infection was related to a therapeutic procedure, whereas nearly half of the PVE patients had clinical evidence of an extracardiac infection at the time of open-heart surgery. We emphasize the need for good pre- and postoperative surveillance to eliminate possible predisposing infections and appropriate antibiotic prophylaxis in all patients with valvular disease at times of risk. The survival rate in patients with prosthetic valve endocarditis was highest in those patients who received \"appropriate\" antibiotics and, if significant congestive heart failure was present, surgical intervention was necessary.", "contents": "A comparative study of 48 host valve and 24 prosthetic valve endocarditis cases. Forty-eight cases of host valve endocarditis and 24 cases of prosthetic valve endocarditis occurring during a concurrent period of time were analyzed to assess differences between the two groups. The over-all incidence of prosthetic valve endocarditis during this 40 month period was 4.4%. The symptoms and signs in both groups were similar, except that PVE patients had more frequent occurrences of changing heart murmurs and splenic and cerebral emboli. Spleen scans may be helpful in the diagnosis of selected cases of culture-negative prosthetic valve endocarditis. There was no significant difference between the two groups for the various infecting microorganisms. However, the culture-negative prosthetic valve group had a mortality rate of 77.7% compared to 46.2% for the host valve group. In the HVE patients the oral cavity or urinary tract was the probable source of infection in 50.0% of the patients. In about one third of HVE cases, there was strong evidence that the infection was related to a therapeutic procedure, whereas nearly half of the PVE patients had clinical evidence of an extracardiac infection at the time of open-heart surgery. We emphasize the need for good pre- and postoperative surveillance to eliminate possible predisposing infections and appropriate antibiotic prophylaxis in all patients with valvular disease at times of risk. The survival rate in patients with prosthetic valve endocarditis was highest in those patients who received \"appropriate\" antibiotics and, if significant congestive heart failure was present, surgical intervention was necessary.", "PMID": 961575}
{"id": "pubmed23n0032_4502", "title": "A comparative analysis of four protocols for maximal treadmill stress testing.", "content": "The purpose of this investigation was to compare the results from four commonly used maximal treadmill stress tests: Balke, Bruce, Ellestad, and a continuous multistage running protocol. The results compared serial and maximal heart rate, metabolic demands, and ECG determinations. Fifty-one healthy men, 35 to 55 years of age, volunteered for this study and were dichotomized into trained and untrained subjects. Regression analyses showed all the tests to correlate highly. No significant differences were found between tests at maximum for V02, heart rate, and blood pressure, except for V02 for the Balke as compared to the running protocol (39 vs. 41 ml./Kg-min). The Balke protocol showed lower values at maximum in VE and RP than the other three tests as well as the most gradual rate of progression in MET cost (0.5 METS per minute). The increase for the Bruce and Ellestad tests was from 1 to 1.5 METS per minute, and a rapid initial increase (9 METS in the first 3 minutes) made the running test undesirable as a screening method. Although serial plots of heart rate and MET costs were similar to those previously reported for different population samples, the present data further refined these values. Finally, a nomograph comparing treadmill time and V02, max. for the Balke, Bruce, and Ellestad tests was developed from these data.", "contents": "A comparative analysis of four protocols for maximal treadmill stress testing. The purpose of this investigation was to compare the results from four commonly used maximal treadmill stress tests: Balke, Bruce, Ellestad, and a continuous multistage running protocol. The results compared serial and maximal heart rate, metabolic demands, and ECG determinations. Fifty-one healthy men, 35 to 55 years of age, volunteered for this study and were dichotomized into trained and untrained subjects. Regression analyses showed all the tests to correlate highly. No significant differences were found between tests at maximum for V02, heart rate, and blood pressure, except for V02 for the Balke as compared to the running protocol (39 vs. 41 ml./Kg-min). The Balke protocol showed lower values at maximum in VE and RP than the other three tests as well as the most gradual rate of progression in MET cost (0.5 METS per minute). The increase for the Bruce and Ellestad tests was from 1 to 1.5 METS per minute, and a rapid initial increase (9 METS in the first 3 minutes) made the running test undesirable as a screening method. Although serial plots of heart rate and MET costs were similar to those previously reported for different population samples, the present data further refined these values. Finally, a nomograph comparing treadmill time and V02, max. for the Balke, Bruce, and Ellestad tests was developed from these data.", "PMID": 961576}
{"id": "pubmed23n0032_4503", "title": "The effects of increased blood viscosity on pulmonary vascular resistance.", "content": "The isolated left lower lobes of 15 dogs' lungs were perfused by means of a roller pump with blood at hematocrit values ranging from 31 to 80 per cent. Pressure-flow curves were constructed at blood flow rates from one half to three times the normal flow for the left lower lobe at each hematocrit level. The perfusion pressure was normalized with reference to the normal hematocrit(38 to 48 per cent) and normal blood flow for the left lower lobe (20 ml. per kilogram per minute). From these normalized pressure-flow curves, normalized resistance-flow curves were constructed at different mean hematocrit levels. Regression lines were drawn relating normalized pulmonary vascular resistance to hematocrit at different rates of pulmonary blood flow which might be found in patients with congenital heart disease. It was found that pulmonary vascular resistance rose in an exponential fashion as the hematocrit was increased, and that the blood viscosity determined both the shape of the resistance-flow curve and magnitude of the increase in resistance to pulmonary blood flow, especially when the pulmonary blood flow was less than normal and the hematocrit was greater than 54 per cent. The family of regression lines relating pulmonary vascular resistance to hematocrit at different flow rates may be used clinically in patients with congenital heart disease and polycythemia to determine if an elevated pulmonary vascular resistance is due to increased blood viscosity or obstructive pulmonary vascular disease. It is concluded that an increased blood viscosity due to polycythemia significantly alters the pulmonary hemodynamics of patients with congenital heart disease with either increased or decreased pulmonary blood flow. Increased blood viscosity may play an important part in the early initiation and development of pulmonary arteriosclerosis in patients with transposition of the great arteries.", "contents": "The effects of increased blood viscosity on pulmonary vascular resistance. The isolated left lower lobes of 15 dogs' lungs were perfused by means of a roller pump with blood at hematocrit values ranging from 31 to 80 per cent. Pressure-flow curves were constructed at blood flow rates from one half to three times the normal flow for the left lower lobe at each hematocrit level. The perfusion pressure was normalized with reference to the normal hematocrit(38 to 48 per cent) and normal blood flow for the left lower lobe (20 ml. per kilogram per minute). From these normalized pressure-flow curves, normalized resistance-flow curves were constructed at different mean hematocrit levels. Regression lines were drawn relating normalized pulmonary vascular resistance to hematocrit at different rates of pulmonary blood flow which might be found in patients with congenital heart disease. It was found that pulmonary vascular resistance rose in an exponential fashion as the hematocrit was increased, and that the blood viscosity determined both the shape of the resistance-flow curve and magnitude of the increase in resistance to pulmonary blood flow, especially when the pulmonary blood flow was less than normal and the hematocrit was greater than 54 per cent. The family of regression lines relating pulmonary vascular resistance to hematocrit at different flow rates may be used clinically in patients with congenital heart disease and polycythemia to determine if an elevated pulmonary vascular resistance is due to increased blood viscosity or obstructive pulmonary vascular disease. It is concluded that an increased blood viscosity due to polycythemia significantly alters the pulmonary hemodynamics of patients with congenital heart disease with either increased or decreased pulmonary blood flow. Increased blood viscosity may play an important part in the early initiation and development of pulmonary arteriosclerosis in patients with transposition of the great arteries.", "PMID": 961577}
{"id": "pubmed23n0032_4504", "title": "Comparison of left atrial size and pulmonary capillary pressure with P wave of electrocardiogram.", "content": "The amplitude and duration of P waves in Leads II (P II), P terminal force in V1, (PV1) and the sums of P II and PV1 were compared in 37 subjects with left atrial size obtained by echocardiographic technique in 36 instances and with hemodynamic estimates of pulmonary capillary wedge pressures in 16 cases. The 22 females and 15 males were subdivided into the following groups. Group I, four normal subjects, Group II, 11 patients with predominant aortic insufficiency (two of whom had a mild mitral insufficiency); Group III, 14 patients with mitral valve disease, seven of whom had mitral insufficiency (two with minimal aortic insufficiency) Group IIIa) and seven had mitral stenosis (Group IIIb); Group IV, eight patients with miscellaneous disorders, i.e., coronary artery disease (5), hypertension (2), and idiopathic hypertrophic subaortic stenosis (1). Good correlations were obtained between left atrial size and P in Lead II (P II) (r = 0.74; p less than 0.001) and between P terminal force in V1 (PV1) and left atrial size (r = -0.69; p less than 0.001). In Group IV good correlation between PV1 and atrial size was noted. Some correlation between the sum of P II and PV1 and left atrial size (r = 0.51; p less than 0.02) was noted, but a better correlation was obtained in the patients with aortic insufficiency (r = 0.80; p less than 0.01). Pulmonary capillary wedge pressures were not reflected in changes in P II or PV1, except for the group with mitral stenosis (Group IIIb). Adding P II to PV1 improved the correlation with wedge pressure for the entire group.", "contents": "Comparison of left atrial size and pulmonary capillary pressure with P wave of electrocardiogram. The amplitude and duration of P waves in Leads II (P II), P terminal force in V1, (PV1) and the sums of P II and PV1 were compared in 37 subjects with left atrial size obtained by echocardiographic technique in 36 instances and with hemodynamic estimates of pulmonary capillary wedge pressures in 16 cases. The 22 females and 15 males were subdivided into the following groups. Group I, four normal subjects, Group II, 11 patients with predominant aortic insufficiency (two of whom had a mild mitral insufficiency); Group III, 14 patients with mitral valve disease, seven of whom had mitral insufficiency (two with minimal aortic insufficiency) Group IIIa) and seven had mitral stenosis (Group IIIb); Group IV, eight patients with miscellaneous disorders, i.e., coronary artery disease (5), hypertension (2), and idiopathic hypertrophic subaortic stenosis (1). Good correlations were obtained between left atrial size and P in Lead II (P II) (r = 0.74; p less than 0.001) and between P terminal force in V1 (PV1) and left atrial size (r = -0.69; p less than 0.001). In Group IV good correlation between PV1 and atrial size was noted. Some correlation between the sum of P II and PV1 and left atrial size (r = 0.51; p less than 0.02) was noted, but a better correlation was obtained in the patients with aortic insufficiency (r = 0.80; p less than 0.01). Pulmonary capillary wedge pressures were not reflected in changes in P II or PV1, except for the group with mitral stenosis (Group IIIb). Adding P II to PV1 improved the correlation with wedge pressure for the entire group.", "PMID": 961578}
{"id": "pubmed23n0032_4505", "title": "Rupture of interventricular septum in acute myocardial infarction.", "content": "Three cases of rupture of the interventricular septum occurring coincident with acute inferior myocardial infarction are reported and the relevant literature is reviewed. One patient under-went successful surgical repair and, in addition, had an aneurysmectomy. Clues and pitfalls in diagnosis as well as the possible confusion with ruptured papillary muscle are discussed. Due to a dual blood supply to the interventricular septum, rupture occurs most often in the setting of advanced, diffuse coronary artery disease. Hypertension or other factors may be of lesser importance. Early surgical approach is advocated and may of necessity include infarctectomy, aneurysmectomy, and coronary artery by-pass surgery in addition ot repair of the ruptured interventricular septum. Use of an intraaortic balloon counterpulsator may prove to be of considerable help in the preoperative and postoperative management of these patients.", "contents": "Rupture of interventricular septum in acute myocardial infarction. Three cases of rupture of the interventricular septum occurring coincident with acute inferior myocardial infarction are reported and the relevant literature is reviewed. One patient under-went successful surgical repair and, in addition, had an aneurysmectomy. Clues and pitfalls in diagnosis as well as the possible confusion with ruptured papillary muscle are discussed. Due to a dual blood supply to the interventricular septum, rupture occurs most often in the setting of advanced, diffuse coronary artery disease. Hypertension or other factors may be of lesser importance. Early surgical approach is advocated and may of necessity include infarctectomy, aneurysmectomy, and coronary artery by-pass surgery in addition ot repair of the ruptured interventricular septum. Use of an intraaortic balloon counterpulsator may prove to be of considerable help in the preoperative and postoperative management of these patients.", "PMID": 961580}
{"id": "pubmed23n0032_4506", "title": "Effect of beta-adrenergic blockade with alprenolol on ST-segment depression and circulatory dynamics during exercise in patients with effort angina.", "content": "1. Nine subjects with severe coronary artery disease were studied during graded \"sprint\" and \"steady-state\" exercise before and after intravenous administration of the beta-receptor antagonist alprenolol. During \"sprint\" workload was increased every minute until maximum work capacity (Wmax) was reached. \"Steady-state\" exercise was performed at work rates of 0.250, 0.50, and 0.75 of each subject's sprint Wmax. Variables measured included ST-segment depression, changes in heart rate, blood pressure, respiratory gas exchange, and arterial blood composition. Cardiac output (indirect Fick) was measured during \"steady-state\" exercise. 2. Alprenolol did not alter Wmax during \"sprint\" but reduced the incidence of angina in both types of exercise. After the drug work capacity was limited by symptoms and signs suggestive of mild left ventricular failure. 3. The relationship between workload (normalized in terms of Wmax) and ST-segment depression was curvilinear. Under control conditions a given rate of work during \"steady-state\" exercise was assocaited with more marked ST-segment depression than during \"sprint\". Alprenolol displaced the work-ST-depression curve to the right in each type of exercise; now a given rate of work produced similar ST-depressing during \"steady-state\" and \"sprint\" exercise. 4. Alprenolol attentuated the exercise tachycardia during both types of exercise. Cardiac output was lower in \"steady-state\" exercise after the drug than under control conditions. Metabolic effects included significant reduction in body oxygen consumption after alpreprenolol at 0.25 Wmax and diminished arterial lactate at 0.75 Wmax. The beneficial effects of the drug thus appeared to involve not only cardiac but peripheral effects on beta receptors. 5. Before alprenolol cardiac output was relatively fixed at all workloads, but after the drug there was a work-related rise in output in five out of nine subjects. Comparison with data in normal subjects suggested that in anginal subjects cardiac output at low \"steady-state\" workloads was inappropriately high.", "contents": "Effect of beta-adrenergic blockade with alprenolol on ST-segment depression and circulatory dynamics during exercise in patients with effort angina. 1. Nine subjects with severe coronary artery disease were studied during graded \"sprint\" and \"steady-state\" exercise before and after intravenous administration of the beta-receptor antagonist alprenolol. During \"sprint\" workload was increased every minute until maximum work capacity (Wmax) was reached. \"Steady-state\" exercise was performed at work rates of 0.250, 0.50, and 0.75 of each subject's sprint Wmax. Variables measured included ST-segment depression, changes in heart rate, blood pressure, respiratory gas exchange, and arterial blood composition. Cardiac output (indirect Fick) was measured during \"steady-state\" exercise. 2. Alprenolol did not alter Wmax during \"sprint\" but reduced the incidence of angina in both types of exercise. After the drug work capacity was limited by symptoms and signs suggestive of mild left ventricular failure. 3. The relationship between workload (normalized in terms of Wmax) and ST-segment depression was curvilinear. Under control conditions a given rate of work during \"steady-state\" exercise was assocaited with more marked ST-segment depression than during \"sprint\". Alprenolol displaced the work-ST-depression curve to the right in each type of exercise; now a given rate of work produced similar ST-depressing during \"steady-state\" and \"sprint\" exercise. 4. Alprenolol attentuated the exercise tachycardia during both types of exercise. Cardiac output was lower in \"steady-state\" exercise after the drug than under control conditions. Metabolic effects included significant reduction in body oxygen consumption after alpreprenolol at 0.25 Wmax and diminished arterial lactate at 0.75 Wmax. The beneficial effects of the drug thus appeared to involve not only cardiac but peripheral effects on beta receptors. 5. Before alprenolol cardiac output was relatively fixed at all workloads, but after the drug there was a work-related rise in output in five out of nine subjects. Comparison with data in normal subjects suggested that in anginal subjects cardiac output at low \"steady-state\" workloads was inappropriately high.", "PMID": 961582}
{"id": "pubmed23n0032_4507", "title": "Incidence and morphology of carotid shudders in aortic valve disease.", "content": "The incidence and morphology of shudders in carotid arterial pulse tracings were examined in 73 patients with aortic valve disease documented by cardiac catheterization. Two forms of carotid shudder were recorded: coarse and fine. Shudders were present in 67 per cent of patients with aortic stenosis, 48 per cent of patients with aortic insufficiency, and 57 per cent of patients with mixed aortic stenosis and insufficiency. No significant difference existed among these three groups of patients with respect to the over-all incidence of carotid shudders or with respect to the incidence of coarse or fine shudders. In patients with aortic insufficiency, stroke volume index (Fick) and phonocardiographic systolic ejection murmur amplitude were significantly greater (p less than 0.01, p less than 0.001, respectively) in those with coarse carotid shudders compared with those manifesting fine or absent shudders. Loud, flow-related, systolic ejection murmurs of aortic insufficiency are capable of producing radial vibrations in the aortic wall which are recorded as carotid shudders. The finding of a carotid shudder in a patient with aortic valve disease does not enable the physician to distinguish between stenosis, insufficiency, or mixed stenosis and insufficiency.", "contents": "Incidence and morphology of carotid shudders in aortic valve disease. The incidence and morphology of shudders in carotid arterial pulse tracings were examined in 73 patients with aortic valve disease documented by cardiac catheterization. Two forms of carotid shudder were recorded: coarse and fine. Shudders were present in 67 per cent of patients with aortic stenosis, 48 per cent of patients with aortic insufficiency, and 57 per cent of patients with mixed aortic stenosis and insufficiency. No significant difference existed among these three groups of patients with respect to the over-all incidence of carotid shudders or with respect to the incidence of coarse or fine shudders. In patients with aortic insufficiency, stroke volume index (Fick) and phonocardiographic systolic ejection murmur amplitude were significantly greater (p less than 0.01, p less than 0.001, respectively) in those with coarse carotid shudders compared with those manifesting fine or absent shudders. Loud, flow-related, systolic ejection murmurs of aortic insufficiency are capable of producing radial vibrations in the aortic wall which are recorded as carotid shudders. The finding of a carotid shudder in a patient with aortic valve disease does not enable the physician to distinguish between stenosis, insufficiency, or mixed stenosis and insufficiency.", "PMID": 961583}
{"id": "pubmed23n0032_4508", "title": "Comparison of the effects of atrial and ventricular stimulation on sinus node function in man.", "content": "Although sinus node function has been evaluated during premature atrial stimulation, no study of retrograde ventriculoatrial sinus node activation following premature ventricular stimuli has been reported. The purpose of this study was to investigate the production of compensatory and noncompensatory pauses by premature ventricular contractions through a comparison of the effects of atrial and ventricular stimulation on sinus node function. Eleven patients in sinus rhythm were studied with programmed introduction of premature atrial and ventricular stimuli outside the ventricular vulnerable period. The onset of sinus node reset, duration of return sinus cycle (A2-A3) during reset, and estimated sinoatrial conduction times were recorded. Sinus node function during premature ventricular stimulation was approximated by utilizing the interval between the last sinus beat and onset of retrograde atrial depolarization (A1-A2 interval). The return cycle length (A2-A3) during sinus reset compared at equal A1-A2 intervals was significantly less with ventriculoatrial conduction (1,145 +/- 52 msec. atrial vs. 1,076 +/- 52 msec ventriculoatrial; P less than 0.01 by paired t test) and the estimated sinoatrial conduction time was significantly less with ventriculoatrial conduction (71 +/- 7 msec. atrial vs. 25 +/- 7 msec. ventriculoatrial; P less than 0.01 by paired t test). Ventriculoatrial sinus reset occurred later in the sinus cycle than atrial reset in three of seven patients with sinus reset produced by both atrial and ventricular prematures. This study shows that the effects of ventriculoatrial conduction on sinus node function are significantly different from those of atrial stimulation alone. The return sinus cycle length during reset and estimated sinoatrial conduction time are significantly reduced with ventriculoatrial conduction. Although the zones of sinus reset with atrial and ventricular stimulation are approximately equal, ventriculoatrial depolarization may produce sinus reset later in the sinus cycle in some cases.", "contents": "Comparison of the effects of atrial and ventricular stimulation on sinus node function in man. Although sinus node function has been evaluated during premature atrial stimulation, no study of retrograde ventriculoatrial sinus node activation following premature ventricular stimuli has been reported. The purpose of this study was to investigate the production of compensatory and noncompensatory pauses by premature ventricular contractions through a comparison of the effects of atrial and ventricular stimulation on sinus node function. Eleven patients in sinus rhythm were studied with programmed introduction of premature atrial and ventricular stimuli outside the ventricular vulnerable period. The onset of sinus node reset, duration of return sinus cycle (A2-A3) during reset, and estimated sinoatrial conduction times were recorded. Sinus node function during premature ventricular stimulation was approximated by utilizing the interval between the last sinus beat and onset of retrograde atrial depolarization (A1-A2 interval). The return cycle length (A2-A3) during sinus reset compared at equal A1-A2 intervals was significantly less with ventriculoatrial conduction (1,145 +/- 52 msec. atrial vs. 1,076 +/- 52 msec ventriculoatrial; P less than 0.01 by paired t test) and the estimated sinoatrial conduction time was significantly less with ventriculoatrial conduction (71 +/- 7 msec. atrial vs. 25 +/- 7 msec. ventriculoatrial; P less than 0.01 by paired t test). Ventriculoatrial sinus reset occurred later in the sinus cycle than atrial reset in three of seven patients with sinus reset produced by both atrial and ventricular prematures. This study shows that the effects of ventriculoatrial conduction on sinus node function are significantly different from those of atrial stimulation alone. The return sinus cycle length during reset and estimated sinoatrial conduction time are significantly reduced with ventriculoatrial conduction. Although the zones of sinus reset with atrial and ventricular stimulation are approximately equal, ventriculoatrial depolarization may produce sinus reset later in the sinus cycle in some cases.", "PMID": 961584}
{"id": "pubmed23n0032_4509", "title": "ECG and VCG changes in experimental hemiblock and bifascicular block.", "content": "ECG and VCG changes in QRS configuration before and after left anterior subdivision block (LASB), isolated and combined with right bundle branch block (LASB combined with RBBB), were studied in canine hearts. In another series left posterior subdivision block (LPSB), isolated and combined with right bundle branch block (LPSB combined with RBBB), were also performed. With LASB, the A QRS axis deviated superiorly to the left and the QRS duration showed no significant prolongation. Additional RBBB to the existing LASB, moreover, rotated the A QRS axis counterclockwise, and prolonged the QRS duration significantly. With LPSB, the axis was deviated inferiorly to the right and the QRS duration showed no significant prolongation. Additional RBBB to the existing LPSB rotated the A QRS axis clockwise. The QRS duration showed significant prolongation. Vectorcardiographically, the initial part of the QRS loop did not show any essential changes with either LASB or LPSB. The main and terminal parts of the QRS loop, however, were deviated superiorly and to the left with LASB and inferiorly and to the right with LPSB. No changes were observed in the direction of rotation of QRS loop with either subdivision block. From these results it appears that the changes in the terminal forces are more of importance than the initial forces in the diagnosis of hemiblock.", "contents": "ECG and VCG changes in experimental hemiblock and bifascicular block. ECG and VCG changes in QRS configuration before and after left anterior subdivision block (LASB), isolated and combined with right bundle branch block (LASB combined with RBBB), were studied in canine hearts. In another series left posterior subdivision block (LPSB), isolated and combined with right bundle branch block (LPSB combined with RBBB), were also performed. With LASB, the A QRS axis deviated superiorly to the left and the QRS duration showed no significant prolongation. Additional RBBB to the existing LASB, moreover, rotated the A QRS axis counterclockwise, and prolonged the QRS duration significantly. With LPSB, the axis was deviated inferiorly to the right and the QRS duration showed no significant prolongation. Additional RBBB to the existing LPSB rotated the A QRS axis clockwise. The QRS duration showed significant prolongation. Vectorcardiographically, the initial part of the QRS loop did not show any essential changes with either LASB or LPSB. The main and terminal parts of the QRS loop, however, were deviated superiorly and to the left with LASB and inferiorly and to the right with LPSB. No changes were observed in the direction of rotation of QRS loop with either subdivision block. From these results it appears that the changes in the terminal forces are more of importance than the initial forces in the diagnosis of hemiblock.", "PMID": 961586}
{"id": "pubmed23n0032_4510", "title": "Propranolol in experimental myocardial ischemia: dissociation of effects on contraction and epicardial ST segments.", "content": "Recent studies have suggested that propranolol decreases the extent of myocardial injury in acute ischemia. Although other studies have shown that global myocardial performance is depressed, zonal effects of propranolol in this setting are unknown. Therefore, the effect of propranolol (1.0 mg. per kilogram) was investigated in nine dogs with the use of Walton-Brodie strain gauge arches and local epicardial electrograms (10 to 12 sites). The heart rate effects of propranolol were controlled by atrial pacing. After coronary occlusion, heart rate increased slightly without a significant change in blood pressure. Following the infusion of propranolol, heart rate decreased significantly from 165.0 +/- 3.5 to 126.2 +/- 4.7 beats per minute (p less than 0.001) while both the systolic and diastolic blood pressures showed insignificant changes. After coronary occlusion, nonischemic zone tension showed no significant changes; however, propranolol decreased total tension from 105.2 +/- 2.5 per cent to 66.8 +/- 4.7 (p less than 0.001). Similarly, propranolol further decreased total tension in the border zone from 84.4 +/- 6.7 per cent (p less than .02) to 50.6 +/- 5.1 (p less than 0.01). Ischemic zone tension also fell further (p less than 0.025) after propranolol. Restoration of prepropranolol heart rate had no significant effect on tension development. Following coronary occlusion, sigmaST increased from 5.7 +/- 2.2 to 72.9 +/- 20.1 mv. (p less than 0.001). Coincident with the decrease in heart rate and tension development induced by propranolol, sigmaST decreased to 60.8 +/- 18.8 mv. (p less than 0.05). When the heart rate was restored to prepropranolol level, sigmaST again rose to 73.2 +/- 16.9 mv. (p less than .005). Thus, propranolol does effect an improvement in ischemic injury which is related, at least in part, to the induced decrease in heart rate. A concomitant substantial decrease in local tension development also occurs, however. The latter observations may limit the potential usefulness of propranolol in this setting.", "contents": "Propranolol in experimental myocardial ischemia: dissociation of effects on contraction and epicardial ST segments. Recent studies have suggested that propranolol decreases the extent of myocardial injury in acute ischemia. Although other studies have shown that global myocardial performance is depressed, zonal effects of propranolol in this setting are unknown. Therefore, the effect of propranolol (1.0 mg. per kilogram) was investigated in nine dogs with the use of Walton-Brodie strain gauge arches and local epicardial electrograms (10 to 12 sites). The heart rate effects of propranolol were controlled by atrial pacing. After coronary occlusion, heart rate increased slightly without a significant change in blood pressure. Following the infusion of propranolol, heart rate decreased significantly from 165.0 +/- 3.5 to 126.2 +/- 4.7 beats per minute (p less than 0.001) while both the systolic and diastolic blood pressures showed insignificant changes. After coronary occlusion, nonischemic zone tension showed no significant changes; however, propranolol decreased total tension from 105.2 +/- 2.5 per cent to 66.8 +/- 4.7 (p less than 0.001). Similarly, propranolol further decreased total tension in the border zone from 84.4 +/- 6.7 per cent (p less than .02) to 50.6 +/- 5.1 (p less than 0.01). Ischemic zone tension also fell further (p less than 0.025) after propranolol. Restoration of prepropranolol heart rate had no significant effect on tension development. Following coronary occlusion, sigmaST increased from 5.7 +/- 2.2 to 72.9 +/- 20.1 mv. (p less than 0.001). Coincident with the decrease in heart rate and tension development induced by propranolol, sigmaST decreased to 60.8 +/- 18.8 mv. (p less than 0.05). When the heart rate was restored to prepropranolol level, sigmaST again rose to 73.2 +/- 16.9 mv. (p less than .005). Thus, propranolol does effect an improvement in ischemic injury which is related, at least in part, to the induced decrease in heart rate. A concomitant substantial decrease in local tension development also occurs, however. The latter observations may limit the potential usefulness of propranolol in this setting.", "PMID": 961587}
{"id": "pubmed23n0032_4511", "title": "Cardiovascular effects of ajmaline.", "content": "Ajmaline, a rauwolfia derivative, has been found to possess potent antiarrhythmic effects. The present study has been designed to define the cardiovascular effects of this drug. Hemodynamic studies performed in anesthetized and conscious dogs demonstrated no significant changes in measured hemodynamic parameters at doses equal to or less than 2 mg. per kilogram. Studies in isolated papillary muscle demonstrated no negative inotropic effects until concentrations of 1 X 10(-4). Disparate results were obtained with regard to heart rate reflecting the state of autonomic tone. Electrophysiologic studies in both anesthetized and conscious dogs demonstrated a significant depression of intraventricular conduction with no significant effect on AV nodal conduction; ventricular automaticity was not affected. Ajmaline did not alter digitalis-induced AV nodal conduction prolongation. However, ajmaline dramatically altered or abolished ventricular arrhythmias secondary to acute ischemia. In conclusion, these studies demonstrate that ajmaline specifically depresses intraventricular conduction, suggesting that this drug would be particularly effective in the treatment of re-entrant ventricular arrhythmias.", "contents": "Cardiovascular effects of ajmaline. Ajmaline, a rauwolfia derivative, has been found to possess potent antiarrhythmic effects. The present study has been designed to define the cardiovascular effects of this drug. Hemodynamic studies performed in anesthetized and conscious dogs demonstrated no significant changes in measured hemodynamic parameters at doses equal to or less than 2 mg. per kilogram. Studies in isolated papillary muscle demonstrated no negative inotropic effects until concentrations of 1 X 10(-4). Disparate results were obtained with regard to heart rate reflecting the state of autonomic tone. Electrophysiologic studies in both anesthetized and conscious dogs demonstrated a significant depression of intraventricular conduction with no significant effect on AV nodal conduction; ventricular automaticity was not affected. Ajmaline did not alter digitalis-induced AV nodal conduction prolongation. However, ajmaline dramatically altered or abolished ventricular arrhythmias secondary to acute ischemia. In conclusion, these studies demonstrate that ajmaline specifically depresses intraventricular conduction, suggesting that this drug would be particularly effective in the treatment of re-entrant ventricular arrhythmias.", "PMID": 961588}
{"id": "pubmed23n0032_4512", "title": "Intraventricular trifascicular block verified by His bundle electrocardiography.", "content": "A patient with the combination of right bundle branch block and intermittent left anterior and left posterior hemiblock is presented. His bundle recordings proved that this type of intraventricular conduction defect appeared when the two left fascicles were damaged partially, and to a varying degree. The recordings also revealed a Grade 2 A-V block distal to the A-V node. The mechanism is discussed.", "contents": "Intraventricular trifascicular block verified by His bundle electrocardiography. A patient with the combination of right bundle branch block and intermittent left anterior and left posterior hemiblock is presented. His bundle recordings proved that this type of intraventricular conduction defect appeared when the two left fascicles were damaged partially, and to a varying degree. The recordings also revealed a Grade 2 A-V block distal to the A-V node. The mechanism is discussed.", "PMID": 961589}
{"id": "pubmed23n0032_4513", "title": "Acute coronary occlusion following blunt injury to the chest in the absence of coronary atherosclerosis.", "content": "A 35-year-old man suffered transmural diaphragmatic wall infarction immediately after receiving a nonpenetrating trauma to his chest. During subsequent months crippling angina pectoris developed and coronary arteriography was performed. A complete obstruction of the left circumflex coronary artery was demonstrated 2 cm. distal to its origin. In contrast to most cases previously published, in this case no signs of atherosclerosis were observed in the other coronary arteries. It must be assumed, therefore, that blunt trauma can induce complete coronary occlusion with infarction, even in subjects with normal coronary arteries.", "contents": "Acute coronary occlusion following blunt injury to the chest in the absence of coronary atherosclerosis. A 35-year-old man suffered transmural diaphragmatic wall infarction immediately after receiving a nonpenetrating trauma to his chest. During subsequent months crippling angina pectoris developed and coronary arteriography was performed. A complete obstruction of the left circumflex coronary artery was demonstrated 2 cm. distal to its origin. In contrast to most cases previously published, in this case no signs of atherosclerosis were observed in the other coronary arteries. It must be assumed, therefore, that blunt trauma can induce complete coronary occlusion with infarction, even in subjects with normal coronary arteries.", "PMID": 961590}
{"id": "pubmed23n0032_4514", "title": "Occupational Health/Safety Program Accreditation Commission.", "content": "This presents a brief history of programs for accrediting occupational health activities with discussion of weaknesses and strengths. The relatively short life of the Occupational Health/Safety Program Accreditation Commission is reviewed with emphasis on its charge, goals and accomplishments. The mechanisms for accreditating Occupational Health and Safety Programs is described and some reasons for seeking accreditation are given.", "contents": "Occupational Health/Safety Program Accreditation Commission. This presents a brief history of programs for accrediting occupational health activities with discussion of weaknesses and strengths. The relatively short life of the Occupational Health/Safety Program Accreditation Commission is reviewed with emphasis on its charge, goals and accomplishments. The mechanisms for accreditating Occupational Health and Safety Programs is described and some reasons for seeking accreditation are given.", "PMID": 961597}
{"id": "pubmed23n0032_4515", "title": "Variation in trash composition in raw cottons.", "content": "Raw cotton from 4 machine picked varieties and 2 machine stripped varieties is examined by stereomicroscope and bright-field microscopy for presence of plant trash(bract, leaf, stem, seed, boll, and weed fragments-size range 841-2000mum) that gives rise to cotton dust during yarn manufacturing operations. Bract was found to be the major trash component in all raw cottons examined. Cotton leaf and non-cotton weed materials were also major trash components in most raw cottons.", "contents": "Variation in trash composition in raw cottons. Raw cotton from 4 machine picked varieties and 2 machine stripped varieties is examined by stereomicroscope and bright-field microscopy for presence of plant trash(bract, leaf, stem, seed, boll, and weed fragments-size range 841-2000mum) that gives rise to cotton dust during yarn manufacturing operations. Bract was found to be the major trash component in all raw cottons examined. Cotton leaf and non-cotton weed materials were also major trash components in most raw cottons.", "PMID": 961598}
{"id": "pubmed23n0032_4516", "title": "Plant trash in linters and willowed picker: materials used in the cotton garnetting industry.", "content": "First cut linters and willowed picker, used in cotton garnetting, were examined for content of cotton bract an other plant trash. Bract was found to be a minimal contaminant of the trash of first cut linters. A significant amount of bract (about 9% of the trash) was present in willowed picker. Cotton seed hulls, a valuable oil mill by product, contained an insignificant amount of bract trash.", "contents": "Plant trash in linters and willowed picker: materials used in the cotton garnetting industry. First cut linters and willowed picker, used in cotton garnetting, were examined for content of cotton bract an other plant trash. Bract was found to be a minimal contaminant of the trash of first cut linters. A significant amount of bract (about 9% of the trash) was present in willowed picker. Cotton seed hulls, a valuable oil mill by product, contained an insignificant amount of bract trash.", "PMID": 961599}
{"id": "pubmed23n0032_4517", "title": "A rapid method for the determination of arsenic concentrations in urine at field locations.", "content": "A colorimetric method for arsenic in urine utilizing a portable hood and requiring minimal laboratory facilities is described. Urine samples are combusted in a hot plate-oven with MgO and Mg(No3)2. Arsine is generated from the ashed product and passed into silver diethyldithio-carbamate in pyridine. Inorganic and organic arsenicals in urine at 0.03 ppm with 6% variation is detectable.", "contents": "A rapid method for the determination of arsenic concentrations in urine at field locations. A colorimetric method for arsenic in urine utilizing a portable hood and requiring minimal laboratory facilities is described. Urine samples are combusted in a hot plate-oven with MgO and Mg(No3)2. Arsine is generated from the ashed product and passed into silver diethyldithio-carbamate in pyridine. Inorganic and organic arsenicals in urine at 0.03 ppm with 6% variation is detectable.", "PMID": 961600}
{"id": "pubmed23n0032_4518", "title": "The pregnant woman in industry.", "content": "The intent of this paper is to relate current thought in proper perspective to the mounting concern for the pregnant woman in industry. Embryo toxicology may demonstrate a need for lowered standards of exposure for the woman worker during a pregnancy. Lastly, a plan is offered to obtain data for comparison of female work populations which may or may not be exposed to certain, primarily airborn, contaminants.", "contents": "The pregnant woman in industry. The intent of this paper is to relate current thought in proper perspective to the mounting concern for the pregnant woman in industry. Embryo toxicology may demonstrate a need for lowered standards of exposure for the woman worker during a pregnancy. Lastly, a plan is offered to obtain data for comparison of female work populations which may or may not be exposed to certain, primarily airborn, contaminants.", "PMID": 961601}
{"id": "pubmed23n0032_4519", "title": "The retention of large particles (greater than 2mum) in fibrous filters.", "content": "A simple method of testing fibrous filters to obtain the grade efficiency curve in the particle size range 2-22 mum is described. Dust collected by the method has been used to measure the retention efficency of model filters and it is shown that there is a maximun in the grade efficency curve. The influence of relative humidity on filter behaviour has been investigated. It is shown that the efficiency of filtration in high particle inertia systems increases with increasing relative humidity. It is considered that the reason for this is an increase in particle-fiber adhesion force.", "contents": "The retention of large particles (greater than 2mum) in fibrous filters. A simple method of testing fibrous filters to obtain the grade efficiency curve in the particle size range 2-22 mum is described. Dust collected by the method has been used to measure the retention efficency of model filters and it is shown that there is a maximun in the grade efficency curve. The influence of relative humidity on filter behaviour has been investigated. It is shown that the efficiency of filtration in high particle inertia systems increases with increasing relative humidity. It is considered that the reason for this is an increase in particle-fiber adhesion force.", "PMID": 961602}
{"id": "pubmed23n0032_4520", "title": "An evaluation of fluorescent light sources for use in phototherapy of neonatal jaundice.", "content": "An evaluation of fluorescent lamps for use in the phototherapy of neonatal jaundice was undertaken by making spectral irradiance measurements of each lamp. Conclusions are drawn on the usefulness of current methods of measuring exposure dose to radiation from the lamps. UV hazards from the lamps are evaluated.", "contents": "An evaluation of fluorescent light sources for use in phototherapy of neonatal jaundice. An evaluation of fluorescent lamps for use in the phototherapy of neonatal jaundice was undertaken by making spectral irradiance measurements of each lamp. Conclusions are drawn on the usefulness of current methods of measuring exposure dose to radiation from the lamps. UV hazards from the lamps are evaluated.", "PMID": 961603}
{"id": "pubmed23n0032_4521", "title": "Echocardiogram of the porcine aortic bioprosthesis in the mitral position.", "content": "Echocardiography was performed in 10 consecutive patients who had a clinically normally functioning porcine aortic bioprosthesis in the mitral position. Strong well defined echoes were recorded from the anterior and posterior aspects of the xenograft stent. The maximal separation of the anterior and posterior stent echoes approximated the diameter of the stent at its base. The maximal excursion of the anterior stent was 5 to 10 mm (mean 7.5) with a mean systolic slope of 15 to 35 mm/sec (mean 22.2) and diastolic slope of 11 to 59 mm/sec (mean 21.5). In all 10 patients it was possible to record an anterior xenograft leaflet with anterior movement at the onset of diastole and posterior movement at the onset of systole and with appropriately steep (more than 200 mm/sec) slopes. The diastolic (E-F) slope of the anterior leaflet in 9 of the 10 patients ranged from 9 to 38 mm/sec (mean 19). In 6 of the 10 patients a posterior xenograft leaflet with a movement pattern symmetric with that of the anterior leaflet was recorded. In two patients, the central aortic leaflet was recorded with little diastolic displacement. These two patients also had mild aortic regurgitation, which was associated with diastolic shudder of the xenograft leaflets. Echocardiography was also performed in one patient who was later shown to have a 10 cm3 thrombus on the ventricular surface of a xenograft valve. The echocardiogram in this patient revealed the following abnormalities: (1) excessive anterior stent movement and systolic slope suggesting paravalvular leak in the presence of abnormal cinefluoroscopic valve tilt, and (2) multiple dense nonhomogeneous echoes between the anterior and posterior aspects of the valve stent, with an early diastolic clear space behind the anterior stent and abnormal echoes behind the posterior stent during systole. Echocardiography therefore appears to be useful in evaluating the porcine aortic bioprosthesis in the mitral position.", "contents": "Echocardiogram of the porcine aortic bioprosthesis in the mitral position. Echocardiography was performed in 10 consecutive patients who had a clinically normally functioning porcine aortic bioprosthesis in the mitral position. Strong well defined echoes were recorded from the anterior and posterior aspects of the xenograft stent. The maximal separation of the anterior and posterior stent echoes approximated the diameter of the stent at its base. The maximal excursion of the anterior stent was 5 to 10 mm (mean 7.5) with a mean systolic slope of 15 to 35 mm/sec (mean 22.2) and diastolic slope of 11 to 59 mm/sec (mean 21.5). In all 10 patients it was possible to record an anterior xenograft leaflet with anterior movement at the onset of diastole and posterior movement at the onset of systole and with appropriately steep (more than 200 mm/sec) slopes. The diastolic (E-F) slope of the anterior leaflet in 9 of the 10 patients ranged from 9 to 38 mm/sec (mean 19). In 6 of the 10 patients a posterior xenograft leaflet with a movement pattern symmetric with that of the anterior leaflet was recorded. In two patients, the central aortic leaflet was recorded with little diastolic displacement. These two patients also had mild aortic regurgitation, which was associated with diastolic shudder of the xenograft leaflets. Echocardiography was also performed in one patient who was later shown to have a 10 cm3 thrombus on the ventricular surface of a xenograft valve. The echocardiogram in this patient revealed the following abnormalities: (1) excessive anterior stent movement and systolic slope suggesting paravalvular leak in the presence of abnormal cinefluoroscopic valve tilt, and (2) multiple dense nonhomogeneous echoes between the anterior and posterior aspects of the valve stent, with an early diastolic clear space behind the anterior stent and abnormal echoes behind the posterior stent during systole. Echocardiography therefore appears to be useful in evaluating the porcine aortic bioprosthesis in the mitral position.", "PMID": 961604}
{"id": "pubmed23n0032_4522", "title": "Myocardial infarction in young women.", "content": "This study of 24 women under age 40 years with myocardial infarction demonstrates that even in young women myocardial infarction is most commonly due to coronary atherosclerotic heart disease. Other causes of coronary occlusion were documented in 17% of these patients, indicating that these lesser causes of myocardial infarction are more common in young women than in older persons or in young men. In those patients with coronary atherosclerosis one or more significant risk factors could usually, but not always, be documented. The clinical manifestation of the coronary occlusion in the study group was not unlike its manifestation in groups of different ages or sex, or both.", "contents": "Myocardial infarction in young women. This study of 24 women under age 40 years with myocardial infarction demonstrates that even in young women myocardial infarction is most commonly due to coronary atherosclerotic heart disease. Other causes of coronary occlusion were documented in 17% of these patients, indicating that these lesser causes of myocardial infarction are more common in young women than in older persons or in young men. In those patients with coronary atherosclerosis one or more significant risk factors could usually, but not always, be documented. The clinical manifestation of the coronary occlusion in the study group was not unlike its manifestation in groups of different ages or sex, or both.", "PMID": 961605}
{"id": "pubmed23n0032_4523", "title": "Effect of glucose-insulin-potassium infusions on arteriovenous differences of glucose of free fatty acids and on tissue metabolic changes in dogs with developing myocardial infarction.", "content": "Glucose-insulin-potassium infusions were given to dogs for 6 hours, starting 30 minutes after ligation of the left anterior descending coronary artery. Effects on substrate arteriovenous differences, indexes of ischemic damage and other tissue metabolic changes were compared with changes in dogs with comparable ligations but no infusions. Glucose-insulin-potassium increased the arteriovenous difference of glucose, decreased that of free fatty acid and decreased the arterial free fatty acid/albumin molar ratio. Glucose-insulin-potassium accelerated the rate of fall of the epicardial S-T segment in the infarct zone and prevented the small rise in S-T segment found in the perlinfarct and nonischemic zones. Glucose-insulin-potassium increased the tissue content of glycogen in peripheral infarct, periinfarct and nonischemic zones; increased tissue potassium ion/sodium ion ratios in epicardial infarct zones and in the periinfarct zone; increased adenosine triphosphate in the endocardial infarct zone; decreased inorganic phosphate in the periinfarct and nonischemic zones and in the endocardial infarct zone; and increased lactate in the central infarct and nonischemic zones. The phosphate potential increased in the periinfarct and nonischemic zones. Thus, many glucose-insulin-potassium effects were greater in the peripheral infarct and especially the periinfarct zones. Although increased anaerobic metabolism with lactate production could not be excluded as a mode of action of glucose-insulin-potassium, estimated rates of anaerobic glycolysis were very low, suggesting that other effects such as increased aerobic glycolysis decreased extraction of free fatty acid by the heart, increased tissue glycogen or a \"membrane\" effect might be of major importance.", "contents": "Effect of glucose-insulin-potassium infusions on arteriovenous differences of glucose of free fatty acids and on tissue metabolic changes in dogs with developing myocardial infarction. Glucose-insulin-potassium infusions were given to dogs for 6 hours, starting 30 minutes after ligation of the left anterior descending coronary artery. Effects on substrate arteriovenous differences, indexes of ischemic damage and other tissue metabolic changes were compared with changes in dogs with comparable ligations but no infusions. Glucose-insulin-potassium increased the arteriovenous difference of glucose, decreased that of free fatty acid and decreased the arterial free fatty acid/albumin molar ratio. Glucose-insulin-potassium accelerated the rate of fall of the epicardial S-T segment in the infarct zone and prevented the small rise in S-T segment found in the perlinfarct and nonischemic zones. Glucose-insulin-potassium increased the tissue content of glycogen in peripheral infarct, periinfarct and nonischemic zones; increased tissue potassium ion/sodium ion ratios in epicardial infarct zones and in the periinfarct zone; increased adenosine triphosphate in the endocardial infarct zone; decreased inorganic phosphate in the periinfarct and nonischemic zones and in the endocardial infarct zone; and increased lactate in the central infarct and nonischemic zones. The phosphate potential increased in the periinfarct and nonischemic zones. Thus, many glucose-insulin-potassium effects were greater in the peripheral infarct and especially the periinfarct zones. Although increased anaerobic metabolism with lactate production could not be excluded as a mode of action of glucose-insulin-potassium, estimated rates of anaerobic glycolysis were very low, suggesting that other effects such as increased aerobic glycolysis decreased extraction of free fatty acid by the heart, increased tissue glycogen or a \"membrane\" effect might be of major importance.", "PMID": 961606}
{"id": "pubmed23n0032_4524", "title": "Control of cardiac output in essential hypertension.", "content": "Cardiac and renal hemodynamics and cardiopulmonary and total blood volume were determined in 202 men, 101 with normotension and 101 of the same age with chronic essential hypertension, normal renal function and balanced sodium intake and urinary output. Cardiac output was identical in the two groups, whereas blood pressure and total peripheral resistance were significantly different. The two groups exhibited strong differences in the correlation study: (1) Correlations of blood pressure with, respectively, heart rate, cardiopulmonary blood volume and total blood volume were significant in the normotensive group but not in the hypertensive group. (2) Correlations of cardiac output with, respectively, heart rate, cardiopulmonary blood volume and total blood volume were significant in both groups. (3) Correlations of renal blood flow with, respectively, cardiac output, blood pressure and total blood volume were significant in the hypertensive group but not in the normotensive group. This study provides evidence that: (1) the volume and neural control of blood pressure are disrupted in hypertension whereas control of cardiac output is maintained; and (2) adaptive mechanisms involving renal function are necessary to the maintenance of normal cardiac output in patients with essential hypertension.", "contents": "Control of cardiac output in essential hypertension. Cardiac and renal hemodynamics and cardiopulmonary and total blood volume were determined in 202 men, 101 with normotension and 101 of the same age with chronic essential hypertension, normal renal function and balanced sodium intake and urinary output. Cardiac output was identical in the two groups, whereas blood pressure and total peripheral resistance were significantly different. The two groups exhibited strong differences in the correlation study: (1) Correlations of blood pressure with, respectively, heart rate, cardiopulmonary blood volume and total blood volume were significant in the normotensive group but not in the hypertensive group. (2) Correlations of cardiac output with, respectively, heart rate, cardiopulmonary blood volume and total blood volume were significant in both groups. (3) Correlations of renal blood flow with, respectively, cardiac output, blood pressure and total blood volume were significant in the hypertensive group but not in the normotensive group. This study provides evidence that: (1) the volume and neural control of blood pressure are disrupted in hypertension whereas control of cardiac output is maintained; and (2) adaptive mechanisms involving renal function are necessary to the maintenance of normal cardiac output in patients with essential hypertension.", "PMID": 961607}
{"id": "pubmed23n0032_4525", "title": "Echocardiographic sepctrum of the hypoplastic left heart syndrome: a clinicopathologic correlation in 19 newborns.", "content": "Ultrasonic studies were performed in 19 neonates with the hypoplastic left heart syndrome whose diagnosis was confirmed at angiography or autopsy, or both. The patients were classified in two echocardiographic groups: Group I, 10 infants whose ventricular septum could be recorded, and Group II, 9 infants whose septum could not be recorded. The findings in these groups were compared with those in 60 neonates without congenital heart disease also studied with ultrasound. Two additional neonates who presented with signs of shock were also studied. The diagnostic echocardiographic features of hypoplastic left heart syndrome were: (1)a left ventricular end-diastolic dimension of less than 9 mm; (2)an aortic root diameter of less than 6 mm; (3)a ratio of left ventricular end-diastolic to right ventricular end-diastolic dimension of less than 0.6; and (4)a mitral valve echo that is absent or greatly distorted and of small amplitude. These echocardiographic criteria differed significantly from findings in the normal group (P less than 0.01). Echocardiography proved valuable in neonates with shock. It is a safe, reliable technique that can be used to delineate the intracardiac anatomy in sick neonates with the hypoplastic left heart syndrome.", "contents": "Echocardiographic sepctrum of the hypoplastic left heart syndrome: a clinicopathologic correlation in 19 newborns. Ultrasonic studies were performed in 19 neonates with the hypoplastic left heart syndrome whose diagnosis was confirmed at angiography or autopsy, or both. The patients were classified in two echocardiographic groups: Group I, 10 infants whose ventricular septum could be recorded, and Group II, 9 infants whose septum could not be recorded. The findings in these groups were compared with those in 60 neonates without congenital heart disease also studied with ultrasound. Two additional neonates who presented with signs of shock were also studied. The diagnostic echocardiographic features of hypoplastic left heart syndrome were: (1)a left ventricular end-diastolic dimension of less than 9 mm; (2)an aortic root diameter of less than 6 mm; (3)a ratio of left ventricular end-diastolic to right ventricular end-diastolic dimension of less than 0.6; and (4)a mitral valve echo that is absent or greatly distorted and of small amplitude. These echocardiographic criteria differed significantly from findings in the normal group (P less than 0.01). Echocardiography proved valuable in neonates with shock. It is a safe, reliable technique that can be used to delineate the intracardiac anatomy in sick neonates with the hypoplastic left heart syndrome.", "PMID": 961608}
{"id": "pubmed23n0032_4526", "title": "Surgical correction in complete levotransposition of the great arteries with an unusual subaortic ventricular septal defect.", "content": "Six children with an uncommon variant of complete (that is, physiologically uncorrected) transposition of the great arteries are described. In this malformation, levoposition of the aorta is coincident with situs solitus and concordant atrioventricular relations. All patients underwent successful surgical correction. Four had a ventricular septal defect; in three, the defect was subaortic and because of its unusual anatomic features, a right ventriculotomy was required for repair. Interatrial transposition of venous return was carried out in all cases by insertion of a baffle, as in complete dextrotransposition of the great arteries. The surgical verification of the arterial positions in these cases illustrated the fallibility of the so-called loop rule. The significance of the cases in relation to terminology, classification and morphogenesis of this variant is discussed.", "contents": "Surgical correction in complete levotransposition of the great arteries with an unusual subaortic ventricular septal defect. Six children with an uncommon variant of complete (that is, physiologically uncorrected) transposition of the great arteries are described. In this malformation, levoposition of the aorta is coincident with situs solitus and concordant atrioventricular relations. All patients underwent successful surgical correction. Four had a ventricular septal defect; in three, the defect was subaortic and because of its unusual anatomic features, a right ventriculotomy was required for repair. Interatrial transposition of venous return was carried out in all cases by insertion of a baffle, as in complete dextrotransposition of the great arteries. The surgical verification of the arterial positions in these cases illustrated the fallibility of the so-called loop rule. The significance of the cases in relation to terminology, classification and morphogenesis of this variant is discussed.", "PMID": 961609}
{"id": "pubmed23n0032_4527", "title": "Asymmetric distribution of the pulmonary blood flow between the right and left lungs in d-transposition of the great arteries.", "content": "Pulmonary angiograms, radionuclide lung images and chest roentgenograms were evaluated regarding the incidence, magnitude and natural evolution of maldistribution of the pulmonary blood flow between the lungs in 63 patients with dextrotransposition of the great arteries. Approximately half of these patients had some degree of greater perfusion of the right relative to the left lung. A significant correlation was demonstrated between the incidence of this maldistribution of blood flow and the angulation between the main and the right pulmonary arteries. For any given angulation between these vessels, additional pulmonary stenosis increased the incidence of disparity in perfusion. Our observations suggest the following developmental mechanisms: The maldistribution in flow results from the abnormal rightward inclination of the main pulmonary artery in the transposition malformation which straightens the flow axis from the main to the right pulmonary artery. Under these circumstances the momentum of the blood in the main pulmonary artery carries the blood preferentially into the right pulmonary artery. This momentum is increased when there is stenosis of the left ventricular outflow tract. Consequent differences in the mechanical properties of the two pulmonary vascular beds can increase this maldistribution. The disparity in perfusion between the lungs is not present in newborns with d-transposition, appears to be progressive in severity and in time may result in almost complete cessation of effective perfusion of the left lung. The effect of the Mustard operation on this abnormality of flow is discussed.", "contents": "Asymmetric distribution of the pulmonary blood flow between the right and left lungs in d-transposition of the great arteries. Pulmonary angiograms, radionuclide lung images and chest roentgenograms were evaluated regarding the incidence, magnitude and natural evolution of maldistribution of the pulmonary blood flow between the lungs in 63 patients with dextrotransposition of the great arteries. Approximately half of these patients had some degree of greater perfusion of the right relative to the left lung. A significant correlation was demonstrated between the incidence of this maldistribution of blood flow and the angulation between the main and the right pulmonary arteries. For any given angulation between these vessels, additional pulmonary stenosis increased the incidence of disparity in perfusion. Our observations suggest the following developmental mechanisms: The maldistribution in flow results from the abnormal rightward inclination of the main pulmonary artery in the transposition malformation which straightens the flow axis from the main to the right pulmonary artery. Under these circumstances the momentum of the blood in the main pulmonary artery carries the blood preferentially into the right pulmonary artery. This momentum is increased when there is stenosis of the left ventricular outflow tract. Consequent differences in the mechanical properties of the two pulmonary vascular beds can increase this maldistribution. The disparity in perfusion between the lungs is not present in newborns with d-transposition, appears to be progressive in severity and in time may result in almost complete cessation of effective perfusion of the left lung. The effect of the Mustard operation on this abnormality of flow is discussed.", "PMID": 961610}
{"id": "pubmed23n0032_4528", "title": "Congenitally corrected transposition of the great vessels: localization of the site of complete atrioventricular block using his bundle electrograms.", "content": "Complete atrioventricular block proximal to the bundle of His in a patient with congenitally corrected transposition of the great vessels was documented using His bundle electrograms. The spontaneous rhythnm probably originated from the bundle of His and was responsive to carotid sinus massage, atropine and isometric and treadmill exercise. These electrophysiologic observations are consistent with recent anatomic studies of congenitally corrected transposition of the great vessels.", "contents": "Congenitally corrected transposition of the great vessels: localization of the site of complete atrioventricular block using his bundle electrograms. Complete atrioventricular block proximal to the bundle of His in a patient with congenitally corrected transposition of the great vessels was documented using His bundle electrograms. The spontaneous rhythnm probably originated from the bundle of His and was responsive to carotid sinus massage, atropine and isometric and treadmill exercise. These electrophysiologic observations are consistent with recent anatomic studies of congenitally corrected transposition of the great vessels.", "PMID": 961611}
{"id": "pubmed23n0032_4529", "title": "Pathologic correlations in a case of complete heart block with split His potentials resulting from a stab wound of the heart.", "content": "A 23 year old previously healthy man was stabbed in the anterior chest. This resulted in a ventricular septal defect and complete atrioventricular (A-V) block. The electrocardiogram revealed complete A-V block with a QRS pattern of right bundle branch block. His bundle recordings 26 days later revealed A-V dissociation with split His potentials (P-H1 interval of 100 msec and H2-V interval of 40 msec). During the study the escape QRS shifted from right to left bundle branch block with H2 potentials still preceding each QRS interval with H2-V intervals of 40 msec. A permanent pacemaker was implanted because of persistent congestive heart failure and bradycardia due to A-V block. The patient subsequently became asymptomatic. He died suddenly 3 1/2 years later. Pathologically there were sizable openings in both the tricuspid and mitral valve substance and a ventricular septal defect involving the pars membranacea and part of the adjacent muscular septum. Serial sections of the conduction system revealed total destruction and fibrous replacement of the bifurcation and beginning of the right and left bundle branches and subtotal fibrous replacement of the branching bundle. Thus, the bifurcation of the bundle of His was totally absent at autopsy despite apparent electrophysiologic evidence of its existence 26 days after the stab wound. A possible explanation for this discrepancy is the subsequent fibrosis of the bifurcation produced by hemodynamic changes at the lower margin of the ventricular septal defect.", "contents": "Pathologic correlations in a case of complete heart block with split His potentials resulting from a stab wound of the heart. A 23 year old previously healthy man was stabbed in the anterior chest. This resulted in a ventricular septal defect and complete atrioventricular (A-V) block. The electrocardiogram revealed complete A-V block with a QRS pattern of right bundle branch block. His bundle recordings 26 days later revealed A-V dissociation with split His potentials (P-H1 interval of 100 msec and H2-V interval of 40 msec). During the study the escape QRS shifted from right to left bundle branch block with H2 potentials still preceding each QRS interval with H2-V intervals of 40 msec. A permanent pacemaker was implanted because of persistent congestive heart failure and bradycardia due to A-V block. The patient subsequently became asymptomatic. He died suddenly 3 1/2 years later. Pathologically there were sizable openings in both the tricuspid and mitral valve substance and a ventricular septal defect involving the pars membranacea and part of the adjacent muscular septum. Serial sections of the conduction system revealed total destruction and fibrous replacement of the bifurcation and beginning of the right and left bundle branches and subtotal fibrous replacement of the branching bundle. Thus, the bifurcation of the bundle of His was totally absent at autopsy despite apparent electrophysiologic evidence of its existence 26 days after the stab wound. A possible explanation for this discrepancy is the subsequent fibrosis of the bifurcation produced by hemodynamic changes at the lower margin of the ventricular septal defect.", "PMID": 961612}
{"id": "pubmed23n0032_4530", "title": "Nutritional status measures as predictors of nutritional risk in preschool children.", "content": "Data from 19 dietary and biochemical indices of nutritional status for 419 participants in the Preschool Nutrition Survey were analyzed to determined the smallest number of variables that could be used to identify all children with any of measure below a criterion of adequacy. Serum transferrin iron and dietary iron intake identified 74% of those at nutritional risk. Additional information on dietary calories, calcium, ascorbic acid, thiamin, and vitamin A identified all subjects at risk.", "contents": "Nutritional status measures as predictors of nutritional risk in preschool children. Data from 19 dietary and biochemical indices of nutritional status for 419 participants in the Preschool Nutrition Survey were analyzed to determined the smallest number of variables that could be used to identify all children with any of measure below a criterion of adequacy. Serum transferrin iron and dietary iron intake identified 74% of those at nutritional risk. Additional information on dietary calories, calcium, ascorbic acid, thiamin, and vitamin A identified all subjects at risk.", "PMID": 961618}
{"id": "pubmed23n0032_4531", "title": "Growth and development of three Yugoslav populations in different ecological settings.", "content": "The role of genetical and ecological factors in the physical growth and development was studied in three Yugoslav populations living in different ecological settings. Anthropometric measurements of 6,908 children and young adolescents ages 1 to 18 years were taken and the results were compared in regard to ethnic and ecological characteristics. The evidence is presented that significant improvement in physical characteristics follows the improvement in environmental factors including nutrition. In addition to an increase in body height, changes were also noted in the build of the bony frame of the population living under improved environmental conditions. There was a change in the shape of the skeleton from the squatty appearance of the short population to the more lanky shape of the tall population. It is concluded that systematic periodic collection of the information on child growth could serve as a useful monitoring system for the surveillance of ecological and nutritional trends of a country.", "contents": "Growth and development of three Yugoslav populations in different ecological settings. The role of genetical and ecological factors in the physical growth and development was studied in three Yugoslav populations living in different ecological settings. Anthropometric measurements of 6,908 children and young adolescents ages 1 to 18 years were taken and the results were compared in regard to ethnic and ecological characteristics. The evidence is presented that significant improvement in physical characteristics follows the improvement in environmental factors including nutrition. In addition to an increase in body height, changes were also noted in the build of the bony frame of the population living under improved environmental conditions. There was a change in the shape of the skeleton from the squatty appearance of the short population to the more lanky shape of the tall population. It is concluded that systematic periodic collection of the information on child growth could serve as a useful monitoring system for the surveillance of ecological and nutritional trends of a country.", "PMID": 961619}
{"id": "pubmed23n0032_4532", "title": "Effect of oral contraceptives and vitamin B6 supplements on alanine and glycine metabolism.", "content": "Plasma alanine or glycine concentrations and blood pyruvate were studied before and after oral administration of the two amino acids to women using estrogen-containing oral contraceptives and to a control group of women. Thirty oral contraceptive users and 14 controls received alanine loads (200 mg/kg of body weight). The two groups had similar fasting plasma alanine levels, but the fasting blood pyruvate was elevated in the contraceptive steroid-treated women. After alanine ingestion, the increases in plasma alanine were significantly lower in the oral contraceptive users. Both groups showed a rise in blood pyruvate; there were no differences in the increments above fasting values. Treatment with pyridoxine hydrochloride, 25 mg for 4 weeks, had not effect on the responses of 11 oral contraceptive users to alanine loading. Twenty-four oral contraceptive users and 14 controls were studied before and after a glycine load (140 mg/kg of body weight). The fasting plasma glycine levels were similar, but lesser increases after ingestion of the amino acid occurred in the oral contraceptive-treated group. Blood pyruvate concentrations in both oral contraceptive users and controls were unaffected by oral glycine loads.", "contents": "Effect of oral contraceptives and vitamin B6 supplements on alanine and glycine metabolism. Plasma alanine or glycine concentrations and blood pyruvate were studied before and after oral administration of the two amino acids to women using estrogen-containing oral contraceptives and to a control group of women. Thirty oral contraceptive users and 14 controls received alanine loads (200 mg/kg of body weight). The two groups had similar fasting plasma alanine levels, but the fasting blood pyruvate was elevated in the contraceptive steroid-treated women. After alanine ingestion, the increases in plasma alanine were significantly lower in the oral contraceptive users. Both groups showed a rise in blood pyruvate; there were no differences in the increments above fasting values. Treatment with pyridoxine hydrochloride, 25 mg for 4 weeks, had not effect on the responses of 11 oral contraceptive users to alanine loading. Twenty-four oral contraceptive users and 14 controls were studied before and after a glycine load (140 mg/kg of body weight). The fasting plasma glycine levels were similar, but lesser increases after ingestion of the amino acid occurred in the oral contraceptive-treated group. Blood pyruvate concentrations in both oral contraceptive users and controls were unaffected by oral glycine loads.", "PMID": 961624}
{"id": "pubmed23n0032_4533", "title": "Postprandial glucose, insulin, free fatty acid and growth hormone responses in children consuming all the day's protein in one meal.", "content": "BLOOD GLUCOSE, INSULIN (IRI), growth hormone, and plasma free fatty acids (FFA) were determined in six children consuming a diet of uneven distribution of protein relative to energy (study period). Preprandial and postprandial samples surrounding the 8 AM protein-free feeding and the 3 PM feeding containing all the day's protein were compared with values obtained in the same children similarly sampled while consuming an isonitrogenous isoenergetic diet of even protein distribution (control period). After the 8 AM feeding during the study period there was a mean maximal rise of blood glucose at 30 min of 51 mg/dl compared with a rise of 16 mg/dl during the control period. Glucose remained significantly elevated above fasting values at 120 min during the study but not the control period. IRI response after the 8 AM feeding was significantly greater and suppression of FFA was more marked during the study than during the control period. Glucose concentration 30 min after the 3 PM feeding was significantly lower during the study period than during the control period. A peak value occurred at 60 min during the study period which was equal to the 30 min peak control value. Despite the slower elevation of blood glucose during the study period, IRI rose at 30 min, possibly related to a larger influx of amino acids from the protein-containing meal. FFA rose at 30 and 60 min and were then suppressed by the slowly rising blood glucose. Growth hormone after both meals while consuming both diets was variable but considered normal. The qualitative changes in glucose-IRI-FFA responses were for the most part attributable to differences in the test meals and suggested little long-term adaptation to the uneven protein distribution diet.", "contents": "Postprandial glucose, insulin, free fatty acid and growth hormone responses in children consuming all the day's protein in one meal. BLOOD GLUCOSE, INSULIN (IRI), growth hormone, and plasma free fatty acids (FFA) were determined in six children consuming a diet of uneven distribution of protein relative to energy (study period). Preprandial and postprandial samples surrounding the 8 AM protein-free feeding and the 3 PM feeding containing all the day's protein were compared with values obtained in the same children similarly sampled while consuming an isonitrogenous isoenergetic diet of even protein distribution (control period). After the 8 AM feeding during the study period there was a mean maximal rise of blood glucose at 30 min of 51 mg/dl compared with a rise of 16 mg/dl during the control period. Glucose remained significantly elevated above fasting values at 120 min during the study but not the control period. IRI response after the 8 AM feeding was significantly greater and suppression of FFA was more marked during the study than during the control period. Glucose concentration 30 min after the 3 PM feeding was significantly lower during the study period than during the control period. A peak value occurred at 60 min during the study period which was equal to the 30 min peak control value. Despite the slower elevation of blood glucose during the study period, IRI rose at 30 min, possibly related to a larger influx of amino acids from the protein-containing meal. FFA rose at 30 and 60 min and were then suppressed by the slowly rising blood glucose. Growth hormone after both meals while consuming both diets was variable but considered normal. The qualitative changes in glucose-IRI-FFA responses were for the most part attributable to differences in the test meals and suggested little long-term adaptation to the uneven protein distribution diet.", "PMID": 961625}
{"id": "pubmed23n0032_4534", "title": "Anti-platelet antibody determination by platelet factor 3 assay.", "content": "Anti-platelet antibody determinations by a recently introduced platelet factor 3 assay (Pf-3 assay) were evaluated in 159 specimens from 95 patients who had low platelet counts. As a standard procedure, the 51Cr lysis test (51Cr test) was used. The 51Cr test and Pf-3 assay had an excellent correlation (results agreed for 80.5% of 159 specimens tested). Overall positive results for the 159 specimens were 27.7% by the 51Cr test and 22.0% by the Pf-3 assay. A significantly high incidence of anti-platelet antibodies (71.4%) was found in patients who had received more than 10 units of blood transfusions, in contrast to a non-transfused group, which had an incidence of 6.3%. The difference between transfused and non-transfused groups was significant (x2 = 11.854, p less than 0.001). The Pf-3 assay is easy to perform in most laboratories and yet sensitive enough to detect anti-platelet antibodies that were detectable by the 51Cr test.", "contents": "Anti-platelet antibody determination by platelet factor 3 assay. Anti-platelet antibody determinations by a recently introduced platelet factor 3 assay (Pf-3 assay) were evaluated in 159 specimens from 95 patients who had low platelet counts. As a standard procedure, the 51Cr lysis test (51Cr test) was used. The 51Cr test and Pf-3 assay had an excellent correlation (results agreed for 80.5% of 159 specimens tested). Overall positive results for the 159 specimens were 27.7% by the 51Cr test and 22.0% by the Pf-3 assay. A significantly high incidence of anti-platelet antibodies (71.4%) was found in patients who had received more than 10 units of blood transfusions, in contrast to a non-transfused group, which had an incidence of 6.3%. The difference between transfused and non-transfused groups was significant (x2 = 11.854, p less than 0.001). The Pf-3 assay is easy to perform in most laboratories and yet sensitive enough to detect anti-platelet antibodies that were detectable by the 51Cr test.", "PMID": 961626}
{"id": "pubmed23n0032_4535", "title": "The effect of increased contact activation time on the activated partial thromboplastin time.", "content": "With the kaolin-cephalin activated partial thromboplastin time technic, the plasmas of persons who have Fletcher factor deficiency have shown considerable shortening of clotting times when contact activation has been lengthened from 3 (PTT-3) to 10 minutes (PTT-10). The authors demonstrate that in plasma of most normal individuals, and in coagulopathies of other sorts, only slight shortening usually occurs. Abnormal shortening occurs in plasmas of a few otherwise normal people, the \"slow activators,\" and patients receiving coumarin drugs, who have greatly prolonged prothrombin times. Longer activation may produce greatly prolonged PTT's in plasmas containing heparin in relatively high concentrations. The authors discuss the significance of these findings.", "contents": "The effect of increased contact activation time on the activated partial thromboplastin time. With the kaolin-cephalin activated partial thromboplastin time technic, the plasmas of persons who have Fletcher factor deficiency have shown considerable shortening of clotting times when contact activation has been lengthened from 3 (PTT-3) to 10 minutes (PTT-10). The authors demonstrate that in plasma of most normal individuals, and in coagulopathies of other sorts, only slight shortening usually occurs. Abnormal shortening occurs in plasmas of a few otherwise normal people, the \"slow activators,\" and patients receiving coumarin drugs, who have greatly prolonged prothrombin times. Longer activation may produce greatly prolonged PTT's in plasmas containing heparin in relatively high concentrations. The authors discuss the significance of these findings.", "PMID": 961627}
{"id": "pubmed23n0032_4536", "title": "Transfusion reaction with pulmonary infiltration associated with HL-A-specific leukocyte antibodies.", "content": "A case of a non-hemolytic transfusion reaction with pulmonary infiltration secondary to leukocyte antibodies is described, and previously reported cases are reviewed. This type of reaction can be diagnosed at the bedside when a patient develops fever, hypotension and dyspnea within a few hours following transfusion of whole blood or a plasma product. The roentgenogram of the chest shows pulmonary infiltrates with a normal cardiac silhouette constituting non-cardiac pulmonary edema. To provide laboratory confirmation of this reaction, it is essential to search for leukocyte antibodies by both leukoagglutinin and cytotoxic technics, as well as to determine HL-A phenotypes of both donor and recipient. As the plasma products involved usually come from multiparous women, donor parity should be a routine question in the donor interview in transfusion services. To prevent this reaction, which may prove fatal, blood donated by women who have two or more children should be used for packed cells only.", "contents": "Transfusion reaction with pulmonary infiltration associated with HL-A-specific leukocyte antibodies. A case of a non-hemolytic transfusion reaction with pulmonary infiltration secondary to leukocyte antibodies is described, and previously reported cases are reviewed. This type of reaction can be diagnosed at the bedside when a patient develops fever, hypotension and dyspnea within a few hours following transfusion of whole blood or a plasma product. The roentgenogram of the chest shows pulmonary infiltrates with a normal cardiac silhouette constituting non-cardiac pulmonary edema. To provide laboratory confirmation of this reaction, it is essential to search for leukocyte antibodies by both leukoagglutinin and cytotoxic technics, as well as to determine HL-A phenotypes of both donor and recipient. As the plasma products involved usually come from multiparous women, donor parity should be a routine question in the donor interview in transfusion services. To prevent this reaction, which may prove fatal, blood donated by women who have two or more children should be used for packed cells only.", "PMID": 961628}
{"id": "pubmed23n0032_4537", "title": "Electronic platelet counts with the Coulter counter. Reassessment of a correction factor.", "content": "Platelet counts are determined on the Coulter electronic counter by counting the diluted platelet-rich plasma obtained by sedimentation or centrifugation of whole blood. In calculating the whole-blood platelet count, an empirical correction factor for platelet-free plasma trapped by sedimented erythrocytes has been recommended, and a widely-distributed circular slide rule calculator incorporates the correction factor. In this study, visual and electronic platelet counts were compared in 100 specimens with counts ranging from 10 to 1,100 X 10(3) per mul and hematocrits ranging from 17.5 to 48.5%. Platelet-rich plasma samples prepared by a centrifugation method (Plateletfuge) gave machine counts in close agreement with those of samples prepared by sedimentation. Whole-blood platelet counts determined with the circular calculator were consistently lower than visual counts, with an average difference of -17%. The electronic counts were recalculated after elimination of the correction factor, and agreement then improved to an average difference of only +1.6%. The correction factor for trapped platelet-free plasma leads to erroneously low values and should not be used.", "contents": "Electronic platelet counts with the Coulter counter. Reassessment of a correction factor. Platelet counts are determined on the Coulter electronic counter by counting the diluted platelet-rich plasma obtained by sedimentation or centrifugation of whole blood. In calculating the whole-blood platelet count, an empirical correction factor for platelet-free plasma trapped by sedimented erythrocytes has been recommended, and a widely-distributed circular slide rule calculator incorporates the correction factor. In this study, visual and electronic platelet counts were compared in 100 specimens with counts ranging from 10 to 1,100 X 10(3) per mul and hematocrits ranging from 17.5 to 48.5%. Platelet-rich plasma samples prepared by a centrifugation method (Plateletfuge) gave machine counts in close agreement with those of samples prepared by sedimentation. Whole-blood platelet counts determined with the circular calculator were consistently lower than visual counts, with an average difference of -17%. The electronic counts were recalculated after elimination of the correction factor, and agreement then improved to an average difference of only +1.6%. The correction factor for trapped platelet-free plasma leads to erroneously low values and should not be used.", "PMID": 961629}
{"id": "pubmed23n0032_4538", "title": "Uterine tumors resembling ovarian sex-cord tumors. A clinicopathologic analysis of fourteen cases.", "content": "Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized endometrial stromal tumor except for focal epithelial-like differentiation resembling that seen in sex-cord tumors. These tumors were mostly poorly circumscribed large mural masses. The focal sex-cord-like differentiation was manifested by cords, trabeculae, nests and tubules of epithelial-like cells; eosinophilic bodies resembling Call-Exner bodies were seen in one case. Five of the six patients with tumors of this type have been followed for postoperative intervals of 22 months to 15 years, and pelvic recurrences have developed in three. The tumors in eight cases (Group II) differed from those in Group I by having a predominant or exclusive pattern reminiscent of an ovarian sex-cord tumor. These tumors, most of which were single, well-circumscribed, yellow to tan, mural masses 4 to 10 cm in diameter, appeared benign, with no recurrence during postoperative periods of four months to seven years. The relations of the two groups of tumors to one another and to previously described similar tumors. including so-called granulosa-cell tumors and \"plexiform tumors\" of the uterus, are discussed, and an origin from endometrial stromal cells is postulated.", "contents": "Uterine tumors resembling ovarian sex-cord tumors. A clinicopathologic analysis of fourteen cases. Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized endometrial stromal tumor except for focal epithelial-like differentiation resembling that seen in sex-cord tumors. These tumors were mostly poorly circumscribed large mural masses. The focal sex-cord-like differentiation was manifested by cords, trabeculae, nests and tubules of epithelial-like cells; eosinophilic bodies resembling Call-Exner bodies were seen in one case. Five of the six patients with tumors of this type have been followed for postoperative intervals of 22 months to 15 years, and pelvic recurrences have developed in three. The tumors in eight cases (Group II) differed from those in Group I by having a predominant or exclusive pattern reminiscent of an ovarian sex-cord tumor. These tumors, most of which were single, well-circumscribed, yellow to tan, mural masses 4 to 10 cm in diameter, appeared benign, with no recurrence during postoperative periods of four months to seven years. The relations of the two groups of tumors to one another and to previously described similar tumors. including so-called granulosa-cell tumors and \"plexiform tumors\" of the uterus, are discussed, and an origin from endometrial stromal cells is postulated.", "PMID": 961630}
{"id": "pubmed23n0032_4539", "title": "Brain-stem and adrenal abnormalities in the sudden-infant-death syndrome.", "content": "Fourteen of 28 sudden-infant-death syndrome (SIDS) victims had an abnormal proliferation of astroglial fibers in their brain stems and an enlarged mass of chromaffin cells in their adrenal medullas. Both of these abnormalities were associated with greater than normal muscle in the small pulmonary arteries, abnormal brown fat retention, and retained extramedullary erythropoiesis. These latter three findings are probably evidences of chronic alveolar hypoventilation and hypoxemia. The brain stem and adrenal abnormalities are probably also secondary to chronic hypoxemia.", "contents": "Brain-stem and adrenal abnormalities in the sudden-infant-death syndrome. Fourteen of 28 sudden-infant-death syndrome (SIDS) victims had an abnormal proliferation of astroglial fibers in their brain stems and an enlarged mass of chromaffin cells in their adrenal medullas. Both of these abnormalities were associated with greater than normal muscle in the small pulmonary arteries, abnormal brown fat retention, and retained extramedullary erythropoiesis. These latter three findings are probably evidences of chronic alveolar hypoventilation and hypoxemia. The brain stem and adrenal abnormalities are probably also secondary to chronic hypoxemia.", "PMID": 961631}
{"id": "pubmed23n0032_4540", "title": "Subacute myelo-optico-neuropathy (SMON) following pneumonitis. Serologic confirmation three and a half years after death.", "content": "A case of fulminant subacute myelooptico-neuropathy that occurred in a native American women following pneumonitis instead of the usual gastrointestinal dysfunction is reported. After the acute stage, which left the patient paraplegic and partially blind, she suffered an exacerbation which resulted in her death 11 months after the onset of illness. Autopsy disclosed symmetric optic and spinal demyelination. Neutralizing antibody titer against subacute, myelo-optico-neuropathy virus was determined on postmortem serum 3 1/2 years after death and found significantly elevated.", "contents": "Subacute myelo-optico-neuropathy (SMON) following pneumonitis. Serologic confirmation three and a half years after death. A case of fulminant subacute myelooptico-neuropathy that occurred in a native American women following pneumonitis instead of the usual gastrointestinal dysfunction is reported. After the acute stage, which left the patient paraplegic and partially blind, she suffered an exacerbation which resulted in her death 11 months after the onset of illness. Autopsy disclosed symmetric optic and spinal demyelination. Neutralizing antibody titer against subacute, myelo-optico-neuropathy virus was determined on postmortem serum 3 1/2 years after death and found significantly elevated.", "PMID": 961632}
{"id": "pubmed23n0032_4541", "title": "Hodgkin's disease in American Negroes. Histologic classification of the disease in 143 untreated patients, and age distribution.", "content": "The Rye histologic classification of Hodgkin's disease has been applied to 143 previously untreated cases of Hodgkin's disease in Negro patients seen in four hospitals in Washington, D.C.,during a 16-year period (1959-1974). The frequencies and age distributions of histologic subtypes were compared with those in American and two African series. Those histologic subtypes associated with poor prognoses (mixed-cellularity and lymphocytic depletion) predominated in American Negroes and in Negroes from Ibadan, Nigeria, and Kampala, Uganda. In an American series from Connecticut (approximately 98% Caucasian) the histologic subtypes lymphocytic predominance and nodular sclerosis were preponderant. There were statistically significantly less of the nodular sclerosis subtype (P less than 0.005), and more of the mixed-cellularity subtype in American Negroes compared with the Connecticut series (P less than 0.001). In contrast to findings in predominantly Caucasian populations, there was a predilection of the nodular sclerosis subtype for males in American and in African Negroes. This study showed that Hodgkin's disease in American Negroes epidemiologically corresponds to the so-called \"intermediate pattern\" of the disease.", "contents": "Hodgkin's disease in American Negroes. Histologic classification of the disease in 143 untreated patients, and age distribution. The Rye histologic classification of Hodgkin's disease has been applied to 143 previously untreated cases of Hodgkin's disease in Negro patients seen in four hospitals in Washington, D.C.,during a 16-year period (1959-1974). The frequencies and age distributions of histologic subtypes were compared with those in American and two African series. Those histologic subtypes associated with poor prognoses (mixed-cellularity and lymphocytic depletion) predominated in American Negroes and in Negroes from Ibadan, Nigeria, and Kampala, Uganda. In an American series from Connecticut (approximately 98% Caucasian) the histologic subtypes lymphocytic predominance and nodular sclerosis were preponderant. There were statistically significantly less of the nodular sclerosis subtype (P less than 0.005), and more of the mixed-cellularity subtype in American Negroes compared with the Connecticut series (P less than 0.001). In contrast to findings in predominantly Caucasian populations, there was a predilection of the nodular sclerosis subtype for males in American and in African Negroes. This study showed that Hodgkin's disease in American Negroes epidemiologically corresponds to the so-called \"intermediate pattern\" of the disease.", "PMID": 961633}
{"id": "pubmed23n0032_4542", "title": "The significance of focal glomerular sclerosis in children who have nephrotic syndrome.", "content": "This study was undertaken to learn the significance of focal glomerular sclerosis in children who have nephrotic syndrome. Tissue obtained by percutaneous renal biopsy 10-15 years previously was re-examined. Initially, two of the 29 biopsy specimens contained focal segmental hyalinosis or sclerosis and five of the 29 had focal glomerular obsolescence. The paraffin blocks were serially sectioned and examined. Following this procedure, seven of the 29 biopsies had focal segmented hyalinosis and 16 of the 29 had focal glomerular obsolescence. The percentages of focal segmental hyalinosis and focal glomerular obsolescence were recorded. Only those patients whose focal segmental hyalinosis exceeded 2% progressed to renal failure. Age-matched autopsy material from patients dying without renal dysfunction was used as a control. Focal glomerular sclerosis was seen in 75.8% of the control specimens, although few glomeruli within each specimen were involved. Focal glomerular sclerosis may be found normally; it may be found in nephrotic children who do not develop renal failure. The quantification of sclerotic lesions may be of prognostic significance in childhood nephrosis.", "contents": "The significance of focal glomerular sclerosis in children who have nephrotic syndrome. This study was undertaken to learn the significance of focal glomerular sclerosis in children who have nephrotic syndrome. Tissue obtained by percutaneous renal biopsy 10-15 years previously was re-examined. Initially, two of the 29 biopsy specimens contained focal segmental hyalinosis or sclerosis and five of the 29 had focal glomerular obsolescence. The paraffin blocks were serially sectioned and examined. Following this procedure, seven of the 29 biopsies had focal segmented hyalinosis and 16 of the 29 had focal glomerular obsolescence. The percentages of focal segmental hyalinosis and focal glomerular obsolescence were recorded. Only those patients whose focal segmental hyalinosis exceeded 2% progressed to renal failure. Age-matched autopsy material from patients dying without renal dysfunction was used as a control. Focal glomerular sclerosis was seen in 75.8% of the control specimens, although few glomeruli within each specimen were involved. Focal glomerular sclerosis may be found normally; it may be found in nephrotic children who do not develop renal failure. The quantification of sclerotic lesions may be of prognostic significance in childhood nephrosis.", "PMID": 961634}
{"id": "pubmed23n0032_4543", "title": "Sparganosis in the United States. Report of a case.", "content": "A 20-year-old Philadelphia woman (recently moved from South Carolina) had sparganosis of her lower anterior abdominal wall. In the United States sparganosis occurs when man enters the life cycle of dog or cat tapeworms of the genus Spirometra. Most of the American cases have been reported from the southeastern United States, and the most probable source of infection is contaminated drinking water. Sporadic cases have also been seen in other parts of the country. If the pathologist is familiar with the morphology of spargana, the gross and histologic features are sufficient for identification of genus Spirometra.", "contents": "Sparganosis in the United States. Report of a case. A 20-year-old Philadelphia woman (recently moved from South Carolina) had sparganosis of her lower anterior abdominal wall. In the United States sparganosis occurs when man enters the life cycle of dog or cat tapeworms of the genus Spirometra. Most of the American cases have been reported from the southeastern United States, and the most probable source of infection is contaminated drinking water. Sporadic cases have also been seen in other parts of the country. If the pathologist is familiar with the morphology of spargana, the gross and histologic features are sufficient for identification of genus Spirometra.", "PMID": 961635}
{"id": "pubmed23n0032_4544", "title": "Ultrastructural changes in aspirin hepatotoxicity.", "content": "A percutaneous liver biopsy was obtained from a 9-year-old boy who had elevated serum transaminase levels while receiving aspirin treatment for rheumatic fever. Electron microscopy showed extensive fine-structure changes of the hepatocytes, similar to those induced by hepatotoxic drugs. These included marked dilatation of rough endoplasmic reticulum, proliferation of smooth endoplasmic reticulum, and mitochondrial abnormalities. These ultrastructural changes could be considered evidence that aspirin is hepatotoxic. The morphologic observations reported confirm the need for monitoring patients receiving systemic aspirin treatment transaminase measurements.", "contents": "Ultrastructural changes in aspirin hepatotoxicity. A percutaneous liver biopsy was obtained from a 9-year-old boy who had elevated serum transaminase levels while receiving aspirin treatment for rheumatic fever. Electron microscopy showed extensive fine-structure changes of the hepatocytes, similar to those induced by hepatotoxic drugs. These included marked dilatation of rough endoplasmic reticulum, proliferation of smooth endoplasmic reticulum, and mitochondrial abnormalities. These ultrastructural changes could be considered evidence that aspirin is hepatotoxic. The morphologic observations reported confirm the need for monitoring patients receiving systemic aspirin treatment transaminase measurements.", "PMID": 961636}
{"id": "pubmed23n0032_4545", "title": "An evaluation of the usefulness of amylase isozyme differentiation in patients with hyperamylasemia.", "content": "Amylase assays measure total activity without differentiating the relative contributions of pancreatic- and salivary-type amylase isozymes. Since polyacrylamide electrophoresis allows identification of salivary-and pancreatic-type isoxymes and their respective variants, serum and urine specimens from patients with the clinical diagnoses of mumps (4), pancreatitis (16), or undiagnosed hyperamylasemias (5) were compared with specimens from control subjects. Patients with mumps had elevations of salivary-type isozymes, while those with pancreatitis had elevations of pancreatic-type isozymes. Elevation of salivary-type isozymes was identified in the five patients who had undiagnosed hyperamylasemias; among these, the isozymes of two originated in neoplastic ovarian tissue and those of three, probably in the salivary glands. Amylase isozyme differentiation cannot unamibiguously identify the tissue source of hyperamylasemia. However, in patients whose hyperamylasemia is of unknown etiology or who respond atypically to therapy, amylase electrophoresis provides identification of the elevated isozyme type, thus providing the basis for the rational selection of further diagnostic procedures.", "contents": "An evaluation of the usefulness of amylase isozyme differentiation in patients with hyperamylasemia. Amylase assays measure total activity without differentiating the relative contributions of pancreatic- and salivary-type amylase isozymes. Since polyacrylamide electrophoresis allows identification of salivary-and pancreatic-type isoxymes and their respective variants, serum and urine specimens from patients with the clinical diagnoses of mumps (4), pancreatitis (16), or undiagnosed hyperamylasemias (5) were compared with specimens from control subjects. Patients with mumps had elevations of salivary-type isozymes, while those with pancreatitis had elevations of pancreatic-type isozymes. Elevation of salivary-type isozymes was identified in the five patients who had undiagnosed hyperamylasemias; among these, the isozymes of two originated in neoplastic ovarian tissue and those of three, probably in the salivary glands. Amylase isozyme differentiation cannot unamibiguously identify the tissue source of hyperamylasemia. However, in patients whose hyperamylasemia is of unknown etiology or who respond atypically to therapy, amylase electrophoresis provides identification of the elevated isozyme type, thus providing the basis for the rational selection of further diagnostic procedures.", "PMID": 961637}
{"id": "pubmed23n0032_4546", "title": "Simultaneous determination of plasma aldosterone, corticosterone, 11-deoxycorticosterone, 18-hydroxydeoxycorticosterone, cortisol, and 11-deoxycortisol. Methods and applications.", "content": "A method for the simultaneous determination of aldosterone, corticosterone (B), 11-deoxycorticosterone (DOC), 18-hydroxy-11-deoxycorticosterone (18-OH-DOC), cortisol, and 11-deoxycortisol in a single 1-ml sample of plasma is described. The method is applicable to both man and experimental animal. After extraction and purification by thin-layer chromatography (TLC), aldosterone was determined by radioimmunoassay (RIA) employing antibodies to aldosterone: B, DOC, cortisol and 11-deoxycortisol were determined by RIA employing antibodies to corticosterone; 18-OH-DOC was obtained colorimetrically using the Porter-Silber reagent. Recoveries after extraction and chromatography were: aldosterone 71% +/- 9.4 SD; B 81% +/- 10.2; DOC 73% +/- 8.4; cortisol 65% +/- 7.2; 11-deoxycortisol 74% +/- 6.3 using labeled steroids; 18-OH-DOC 82% +/- 8.7 using inert steroid. The method has a high specificity and is reproducible and accurate.", "contents": "Simultaneous determination of plasma aldosterone, corticosterone, 11-deoxycorticosterone, 18-hydroxydeoxycorticosterone, cortisol, and 11-deoxycortisol. Methods and applications. A method for the simultaneous determination of aldosterone, corticosterone (B), 11-deoxycorticosterone (DOC), 18-hydroxy-11-deoxycorticosterone (18-OH-DOC), cortisol, and 11-deoxycortisol in a single 1-ml sample of plasma is described. The method is applicable to both man and experimental animal. After extraction and purification by thin-layer chromatography (TLC), aldosterone was determined by radioimmunoassay (RIA) employing antibodies to aldosterone: B, DOC, cortisol and 11-deoxycortisol were determined by RIA employing antibodies to corticosterone; 18-OH-DOC was obtained colorimetrically using the Porter-Silber reagent. Recoveries after extraction and chromatography were: aldosterone 71% +/- 9.4 SD; B 81% +/- 10.2; DOC 73% +/- 8.4; cortisol 65% +/- 7.2; 11-deoxycortisol 74% +/- 6.3 using labeled steroids; 18-OH-DOC 82% +/- 8.7 using inert steroid. The method has a high specificity and is reproducible and accurate.", "PMID": 961638}
{"id": "pubmed23n0032_4547", "title": "Changes in serum chemical values as a result of prolonged contact with the clot.", "content": "Changes in serum biochemical values as a function of serum-clot contact time were investigated. Time extending to 48 hours were studied by drawing sets of six specimens from ten subjects. Glucose, lactic dehydrogenase, the transaminases, potassium, alkaline phosphatase, and iron showed significant changes. Other common tests, including electrophoretic separations, were not affected.", "contents": "Changes in serum chemical values as a result of prolonged contact with the clot. Changes in serum biochemical values as a function of serum-clot contact time were investigated. Time extending to 48 hours were studied by drawing sets of six specimens from ten subjects. Glucose, lactic dehydrogenase, the transaminases, potassium, alkaline phosphatase, and iron showed significant changes. Other common tests, including electrophoretic separations, were not affected.", "PMID": 961639}
{"id": "pubmed23n0032_4548", "title": "Pneumonia and pleural effusion. Association with influenza A virus and Staphylococcus aureus.", "content": "An 8-month-old infant with severe pneumonia and massive pleural effusions was found to have concurrent infections due to Staphylococcus aureus and influenza A virus. This was proved by the isolation of both agents from the pleural fluid. We believe this is the first case of isolation of an influenza virus from pleural effusions.", "contents": "Pneumonia and pleural effusion. Association with influenza A virus and Staphylococcus aureus. An 8-month-old infant with severe pneumonia and massive pleural effusions was found to have concurrent infections due to Staphylococcus aureus and influenza A virus. This was proved by the isolation of both agents from the pleural fluid. We believe this is the first case of isolation of an influenza virus from pleural effusions.", "PMID": 961645}
{"id": "pubmed23n0032_4549", "title": "Empty sella syndrome secondary to intrasellar cyst in adolescence.", "content": "A 15-year-old boy had growth failure and failure of sexual development. The probable onset was at age 10. Endocrine studies showed hypopituitarism with deficiency of growth hormone and follicle-stimulating hormone, an abnormal response to metyrapone, and deficiency of thyroid function. Luteinizing hormone level was in the low-normal range. Posterior pituitary function was normal. Roentgenogram showed a large sella with some destruction of the posterior clinoids. Transsphenoidal exploration was carried out. The sella was empty except for a whitish membrane; no pituitary tissue was seen. The sella was packed with muscle. Recovery was uneventful, and the patient was given replacement therapy. On histologic examination,the cyst wall showed low pseudostratified cuboidal epithelium and occasional squamous metaplasia. Hemosiderin-filled phagocytes and acinar structures were also seen. The diagnosis was probable rupture of an intrasellar epithelial cyst, leading to empty sella syndrome.", "contents": "Empty sella syndrome secondary to intrasellar cyst in adolescence. A 15-year-old boy had growth failure and failure of sexual development. The probable onset was at age 10. Endocrine studies showed hypopituitarism with deficiency of growth hormone and follicle-stimulating hormone, an abnormal response to metyrapone, and deficiency of thyroid function. Luteinizing hormone level was in the low-normal range. Posterior pituitary function was normal. Roentgenogram showed a large sella with some destruction of the posterior clinoids. Transsphenoidal exploration was carried out. The sella was empty except for a whitish membrane; no pituitary tissue was seen. The sella was packed with muscle. Recovery was uneventful, and the patient was given replacement therapy. On histologic examination,the cyst wall showed low pseudostratified cuboidal epithelium and occasional squamous metaplasia. Hemosiderin-filled phagocytes and acinar structures were also seen. The diagnosis was probable rupture of an intrasellar epithelial cyst, leading to empty sella syndrome.", "PMID": 961646}
{"id": "pubmed23n0032_4550", "title": "A case of gonococcal osteomyelitis. A complication of gonococcal arthritis.", "content": "Gonococcal osteomyelitis is a rare complication of gonococcal infections since the advent of antibiotics. It is important that physicians be aware of this potential complication so that it is recognized and appropriate therapy promptly instituted.", "contents": "A case of gonococcal osteomyelitis. A complication of gonococcal arthritis. Gonococcal osteomyelitis is a rare complication of gonococcal infections since the advent of antibiotics. It is important that physicians be aware of this potential complication so that it is recognized and appropriate therapy promptly instituted.", "PMID": 961647}
{"id": "pubmed23n0032_4551", "title": "\"Bridging bronchus\". A previously undescribed airway anomaly.", "content": "This report describes the clinical features and pathologic anatomy seen in an infant with anomalous bronchial branching and other congenital anomalies. The patient died of respiratory insufficiency and infection, possibly fostered by the malformed airway and a coexistent partial anomalous venous return. At autopsy, bronchial drainage of the right middle and lower lobes was provided by a large bronchial branch that originated in the left main-stem bronchus and bridged the mediastinum before entering the contralateral lung. Since crossed anatomic airway relationships after formation of primary branches are imcompatible with current concepts of embryologic lung development, a brief critical review of the pertinent literature is undertaken.", "contents": "\"Bridging bronchus\". A previously undescribed airway anomaly. This report describes the clinical features and pathologic anatomy seen in an infant with anomalous bronchial branching and other congenital anomalies. The patient died of respiratory insufficiency and infection, possibly fostered by the malformed airway and a coexistent partial anomalous venous return. At autopsy, bronchial drainage of the right middle and lower lobes was provided by a large bronchial branch that originated in the left main-stem bronchus and bridged the mediastinum before entering the contralateral lung. Since crossed anatomic airway relationships after formation of primary branches are imcompatible with current concepts of embryologic lung development, a brief critical review of the pertinent literature is undertaken.", "PMID": 961648}
{"id": "pubmed23n0032_4552", "title": "Ocular manifestations of subacute sclerosing panencephalitis.", "content": "We report a case of subacute sclerosing panencephalitis (SSPE) with severe peripheral chorioretinal pigment changes in addition to the more typical pigment clumping in the macula. The differential diagnosis of peripheral inactive chorioretinitis, the typical ocular findings, and clinical course of SSPE are discussed.", "contents": "Ocular manifestations of subacute sclerosing panencephalitis. We report a case of subacute sclerosing panencephalitis (SSPE) with severe peripheral chorioretinal pigment changes in addition to the more typical pigment clumping in the macula. The differential diagnosis of peripheral inactive chorioretinitis, the typical ocular findings, and clinical course of SSPE are discussed.", "PMID": 961649}
{"id": "pubmed23n0032_4553", "title": "Teratogenic effect of anticonvulsant drugs.", "content": "Multiple malformations in all four children of a mother taking anticonvulsant drugs are reported, along with the extended family pedigree.", "contents": "Teratogenic effect of anticonvulsant drugs. Multiple malformations in all four children of a mother taking anticonvulsant drugs are reported, along with the extended family pedigree.", "PMID": 961650}
{"id": "pubmed23n0032_4554", "title": "The prognosis of childhood systemic lupus erythematosus.", "content": "A retrospective analysis of 50 patients with childhood systemic lupus erythematosus seen during the past 13 years shows 35 living patients, 13 dead, and 2 lost to follow-up. Fourteen patients were males and 36 females, with the median age of onset 14 years. Clinical presentation was multisystemic in 31, primarily renal in 12, hematologic in 5, and atypical in 2. Renal involvement occurred at some time in all 50 patients. Central nervous system symptoms were present in 22 of 50. Survival in the present series is compared with previously reported survival models in the pediatric age group.", "contents": "The prognosis of childhood systemic lupus erythematosus. A retrospective analysis of 50 patients with childhood systemic lupus erythematosus seen during the past 13 years shows 35 living patients, 13 dead, and 2 lost to follow-up. Fourteen patients were males and 36 females, with the median age of onset 14 years. Clinical presentation was multisystemic in 31, primarily renal in 12, hematologic in 5, and atypical in 2. Renal involvement occurred at some time in all 50 patients. Central nervous system symptoms were present in 22 of 50. Survival in the present series is compared with previously reported survival models in the pediatric age group.", "PMID": 961658}
{"id": "pubmed23n0032_4555", "title": "Transient hepatomegaly and hypoglycemia. A consequence of malicious insulin administration.", "content": "On two occasions, a 3 1/2-year-old black girl had severe hypoglycemia associated with transient hepatomegaly. The plasma insulin level during the second hypoglycemic episode was excessive and led to a diagnosis of malicious insulin administration. We suggest that plasma be obtained for insulin determination in children with hypoglycemia, and that transient hepatomegaly may be a helpful sign in cases of insulin overdose.", "contents": "Transient hepatomegaly and hypoglycemia. A consequence of malicious insulin administration. On two occasions, a 3 1/2-year-old black girl had severe hypoglycemia associated with transient hepatomegaly. The plasma insulin level during the second hypoglycemic episode was excessive and led to a diagnosis of malicious insulin administration. We suggest that plasma be obtained for insulin determination in children with hypoglycemia, and that transient hepatomegaly may be a helpful sign in cases of insulin overdose.", "PMID": 961662}
{"id": "pubmed23n0032_4556", "title": "Gastric inhibitory polypeptide (GIP). Intestinal distribution and stimulation by amino acids and medium-chain triglycerides.", "content": "Radioimmunoassayable gastric inhibitory polypeptide was measured in extracts of canine antrum, duodenum, jejunum, and ileum. The highest GIP concentrations were found in the duodenum (347 +/- 53 ng/g) and jejunum (300 +/- 68 ng/g). An immunochemical similarity was demonstrable between porcine GIP and canine GIP. Dogs prepared with Mann Bollman fistulae were given an amino acid (AA) mixture or medium-chain triglycerides (MTC) by intraduodenal perfusion. With AA, a peak mean serum concentration of 672 +/- 106 pg/ml was reached 15 min after starting the perfusion. MCT resulted in a peak mean serum GIP concentration of 504 +/- 55 pg/ml 30 min after beginning the perfusion. When compared to results previously reported from this laboratory, AA and MCT are not as potent as corn oil (long-chain triglyceride) or glucose in stimulating GIP release. We conclude: (1) Immunoassayable GIP concentrations are highest in the canine proximal small intestine. (2) AA and MCT are weak stimulants of GIP release in the dog.", "contents": "Gastric inhibitory polypeptide (GIP). Intestinal distribution and stimulation by amino acids and medium-chain triglycerides. Radioimmunoassayable gastric inhibitory polypeptide was measured in extracts of canine antrum, duodenum, jejunum, and ileum. The highest GIP concentrations were found in the duodenum (347 +/- 53 ng/g) and jejunum (300 +/- 68 ng/g). An immunochemical similarity was demonstrable between porcine GIP and canine GIP. Dogs prepared with Mann Bollman fistulae were given an amino acid (AA) mixture or medium-chain triglycerides (MTC) by intraduodenal perfusion. With AA, a peak mean serum concentration of 672 +/- 106 pg/ml was reached 15 min after starting the perfusion. MCT resulted in a peak mean serum GIP concentration of 504 +/- 55 pg/ml 30 min after beginning the perfusion. When compared to results previously reported from this laboratory, AA and MCT are not as potent as corn oil (long-chain triglyceride) or glucose in stimulating GIP release. We conclude: (1) Immunoassayable GIP concentrations are highest in the canine proximal small intestine. (2) AA and MCT are weak stimulants of GIP release in the dog.", "PMID": 961670}
{"id": "pubmed23n0032_4557", "title": "Comprehensive formal models for pancreatic calcium secretion. With reference to \"exchange-diffusion,\" \"two-component,\" and \"unicellular\" models of electrolyte secretion.", "content": "This is a theoretical paper in which comprehensive formal models are developed for all possible modes of entry of calcium into pancreatic juice. The 15 models include entry via: (1) the chloride-rich secretion, (2) the bicarbonate-rich secretion, (3) protein-bound calcium, and (4) interstitial fluid (ISF), plus the various possible combinations of these. Equations are presented for both calcium-volume and calcium-bicarbonate relationships. Each equation is modified as necessary to conform to the three current models for sodium, chloride, and bicarbonate secretion: (1) \"exchange-diffusion,\" (2) \"two-component,\" and (3) \"unicellular\" models. We believe the models provide a sound theoretical framework on which to build an understanding of pancreatic calcium secretion. Subsequent papers will be concerned with the physicochemical state of calcium in the juice, the relative contribution of ISF, and the development of an overall model for pancreatic calcium secretion. The long-term objective is to provide a rational approach for the understanding of pancreatic calcification and stone formation.", "contents": "Comprehensive formal models for pancreatic calcium secretion. With reference to \"exchange-diffusion,\" \"two-component,\" and \"unicellular\" models of electrolyte secretion. This is a theoretical paper in which comprehensive formal models are developed for all possible modes of entry of calcium into pancreatic juice. The 15 models include entry via: (1) the chloride-rich secretion, (2) the bicarbonate-rich secretion, (3) protein-bound calcium, and (4) interstitial fluid (ISF), plus the various possible combinations of these. Equations are presented for both calcium-volume and calcium-bicarbonate relationships. Each equation is modified as necessary to conform to the three current models for sodium, chloride, and bicarbonate secretion: (1) \"exchange-diffusion,\" (2) \"two-component,\" and (3) \"unicellular\" models. We believe the models provide a sound theoretical framework on which to build an understanding of pancreatic calcium secretion. Subsequent papers will be concerned with the physicochemical state of calcium in the juice, the relative contribution of ISF, and the development of an overall model for pancreatic calcium secretion. The long-term objective is to provide a rational approach for the understanding of pancreatic calcification and stone formation.", "PMID": 961671}
{"id": "pubmed23n0032_4558", "title": "A new model of stress ulcer in the rat with pylorus ligation and its pathogenesis.", "content": "Water-immersion stress for 7, 14, or 20 hr consistently induced linear or punctate stress ulcers (mucosal erosions) in the corpus of the stomach in intact rats. When the pylorus of the stomach had been ligated prior to stressing, the stress ulcers changed their morphological feature (mainly punctate and in one place elongated) and location (both in corpus and antrum). Histologically, the stress ulcer developed in the proximal antrum of pylorus ligated rats and penetrated into the muscularis mucosa. Sodium bicarbonate, chlorpromazine, hexamethonium, atropine, metiamide, and bilateral vagotomy markedly inhibited the stress ulcers which developed in the pylorus-ligated rats. Phentolamine and propranolol hardly affected the development of stress ulcers. Amylopectine evoked a new type of stress ulcer in the corpus when it was given to the pylorus-ligated rats.", "contents": "A new model of stress ulcer in the rat with pylorus ligation and its pathogenesis. Water-immersion stress for 7, 14, or 20 hr consistently induced linear or punctate stress ulcers (mucosal erosions) in the corpus of the stomach in intact rats. When the pylorus of the stomach had been ligated prior to stressing, the stress ulcers changed their morphological feature (mainly punctate and in one place elongated) and location (both in corpus and antrum). Histologically, the stress ulcer developed in the proximal antrum of pylorus ligated rats and penetrated into the muscularis mucosa. Sodium bicarbonate, chlorpromazine, hexamethonium, atropine, metiamide, and bilateral vagotomy markedly inhibited the stress ulcers which developed in the pylorus-ligated rats. Phentolamine and propranolol hardly affected the development of stress ulcers. Amylopectine evoked a new type of stress ulcer in the corpus when it was given to the pylorus-ligated rats.", "PMID": 961672}
{"id": "pubmed23n0032_4559", "title": "Myxedema ascites.", "content": "A 74-year-old man presented with mental obtundation and massive ascites without evidence of significant impairment of liver function. Thyroid function studies suggested hypothyroidism. Aspirated ascitic fluid had the characteristics of an exudate. Thyroid replacement therapy resulted in rapid clinical improvement with resolution of the ascites. Prompt recognition of myxedema ascites may prevent the inappropriate use of diuretic agents, therapeutic paracentesis, and sometimes unnecessary laparotomy.", "contents": "Myxedema ascites. A 74-year-old man presented with mental obtundation and massive ascites without evidence of significant impairment of liver function. Thyroid function studies suggested hypothyroidism. Aspirated ascitic fluid had the characteristics of an exudate. Thyroid replacement therapy resulted in rapid clinical improvement with resolution of the ascites. Prompt recognition of myxedema ascites may prevent the inappropriate use of diuretic agents, therapeutic paracentesis, and sometimes unnecessary laparotomy.", "PMID": 961677}
{"id": "pubmed23n0032_4560", "title": "Filiform polyposis of the colon. First report in a case of transmural colitis (Crohn's disease).", "content": "Filiform polyposis of the colon is characterized by multitudinous wormlike projections of mucosa and submucosa, and it has only previously been reported to be an unusual sequel to a prior limited episode of ulcerative colitis. The first reported instance complicating a 16-year course of typical transmural colitis (Crohn's disease) suggests that this benign proliferation may be a nonspecific submucosal response to two separated, but closely subadjacent zones of submucosal inflammation in either type of colitis. Although few cases of filiform polyposis are available for review, there has been no instance associated with carcinoma. The entity in itself does not represent an indication for colectomy.", "contents": "Filiform polyposis of the colon. First report in a case of transmural colitis (Crohn's disease). Filiform polyposis of the colon is characterized by multitudinous wormlike projections of mucosa and submucosa, and it has only previously been reported to be an unusual sequel to a prior limited episode of ulcerative colitis. The first reported instance complicating a 16-year course of typical transmural colitis (Crohn's disease) suggests that this benign proliferation may be a nonspecific submucosal response to two separated, but closely subadjacent zones of submucosal inflammation in either type of colitis. Although few cases of filiform polyposis are available for review, there has been no instance associated with carcinoma. The entity in itself does not represent an indication for colectomy.", "PMID": 961678}
{"id": "pubmed23n0032_4561", "title": "A new substrate for the rapid evaluation of enteric microbial overgrowth.", "content": "The possibility of a new approach to diagnosis of intestinal bacterial overgrowth has been evaluated in laboratory animals. The diagnostic test involves oral administration of an enzyme-labile substrate consisting of para-aminobenzoic acid (PABA) conjugated to a bile acid. In the presence of enteric bacteria, PABA is split from the bile acid and is rapidly absorbed and excreted in the urine. The amount of PABA recovered during the 6 hr following the administered dose of the conjugate may be used as an index of bacterial overgrowth in the upper-gastrointestinal tract. The procedure has been shown to be a reliable index of this condition in laboratory animal models.", "contents": "A new substrate for the rapid evaluation of enteric microbial overgrowth. The possibility of a new approach to diagnosis of intestinal bacterial overgrowth has been evaluated in laboratory animals. The diagnostic test involves oral administration of an enzyme-labile substrate consisting of para-aminobenzoic acid (PABA) conjugated to a bile acid. In the presence of enteric bacteria, PABA is split from the bile acid and is rapidly absorbed and excreted in the urine. The amount of PABA recovered during the 6 hr following the administered dose of the conjugate may be used as an index of bacterial overgrowth in the upper-gastrointestinal tract. The procedure has been shown to be a reliable index of this condition in laboratory animal models.", "PMID": 961679}
{"id": "pubmed23n0032_4562", "title": "The asymptomatic HB6-Ag carrier: auto- and heterologous perils.", "content": "The asympatomatic carrier--usually discovered on offering to be a blood donor--presents a complex health problem which to date has received only \"guarded\" attention. These carriers may be dangerous, because they can transmit the HBs-Ag not only by parenteral but also by \"nonparenteral\" means, due to its presence in urine, feces, saliva, tears, semen, vaginal discharge, menstrual flow, breast milk, joint, ascitic and cerebrospinal fluids. The transmission by sexual contact is well established. Inapparent transmission may also occur from insufficient sterilization of instruments, by tatooing, ear piercing, by barbers, manicurists, dentists and by mosquitoes and bedbugs. Some of the characteristics of an asymptomatic HBs-Ag carrier seem to be: a young male, most often black, from a poor social and hygienic environment with a possible gene dependent immune deficiency state. Our experiences suggest that all newly admitted patients to the hospital personnel should have determinations of the presence of HBs-Ag in their blood, so that adequate steps can be taken to protect their contacts from infection.", "contents": "The asymptomatic HB6-Ag carrier: auto- and heterologous perils. The asympatomatic carrier--usually discovered on offering to be a blood donor--presents a complex health problem which to date has received only \"guarded\" attention. These carriers may be dangerous, because they can transmit the HBs-Ag not only by parenteral but also by \"nonparenteral\" means, due to its presence in urine, feces, saliva, tears, semen, vaginal discharge, menstrual flow, breast milk, joint, ascitic and cerebrospinal fluids. The transmission by sexual contact is well established. Inapparent transmission may also occur from insufficient sterilization of instruments, by tatooing, ear piercing, by barbers, manicurists, dentists and by mosquitoes and bedbugs. Some of the characteristics of an asymptomatic HBs-Ag carrier seem to be: a young male, most often black, from a poor social and hygienic environment with a possible gene dependent immune deficiency state. Our experiences suggest that all newly admitted patients to the hospital personnel should have determinations of the presence of HBs-Ag in their blood, so that adequate steps can be taken to protect their contacts from infection.", "PMID": 961681}
{"id": "pubmed23n0032_4563", "title": "Retrograde pancreatography: new contributions.", "content": "The standard technic for pancreatography was modified by using a metallic guide wire in the proximal half of the cannula so as to regulate pressure independently from that applied manually. The maximum pressure obtained by this method was measured in a polyethylene teflon cannula constructed to correspond to the Wirsung duct. Pressure was five times less when the guide wire was used. Our results in 33 patients correspond to experimental data. Only in 28% of our cases did the amylase rise slightly to a maximum of 290 Somogyi units. Acinar opacification was attempted by adding NaCO3 to a standard dye solution and was obtained in 20% of the cases (5 of 25). Maximum amylase elevation in this group was 263 Somogyi units. No complications resulted from the pressure measurements during pancreatography.", "contents": "Retrograde pancreatography: new contributions. The standard technic for pancreatography was modified by using a metallic guide wire in the proximal half of the cannula so as to regulate pressure independently from that applied manually. The maximum pressure obtained by this method was measured in a polyethylene teflon cannula constructed to correspond to the Wirsung duct. Pressure was five times less when the guide wire was used. Our results in 33 patients correspond to experimental data. Only in 28% of our cases did the amylase rise slightly to a maximum of 290 Somogyi units. Acinar opacification was attempted by adding NaCO3 to a standard dye solution and was obtained in 20% of the cases (5 of 25). Maximum amylase elevation in this group was 263 Somogyi units. No complications resulted from the pressure measurements during pancreatography.", "PMID": 961683}
{"id": "pubmed23n0032_4564", "title": "Endoscopic observation of the gastric mucus in vivo stained with azure A.", "content": "Gastric mucus was stained with Azure A, a cationic dye, which had the highest affinity with macromolecular constituents of the mucus, under such conditions as 0.2% Azure A-0.5% NaHCO3 solution (pH 8.1) in dye concentration, staining for ten minutes, 37 degrees C in reaction temperature and the salt concentration and ionic strength below 6.0 x 10(-2). In rat and resected human stomachs, gastric mucus was clearly stained under these conditions. In human subjects, the in vivo stained muscu was observed endoscopically. The pyloric gland region. The difference was seen in the pattern of the gastric area between the fundic and pyloric gland region. Histological examination revealed that only the mucous layer was stained with Azure A. The stained macromolecules in the mucus and factors affecting the staining were discussed.", "contents": "Endoscopic observation of the gastric mucus in vivo stained with azure A. Gastric mucus was stained with Azure A, a cationic dye, which had the highest affinity with macromolecular constituents of the mucus, under such conditions as 0.2% Azure A-0.5% NaHCO3 solution (pH 8.1) in dye concentration, staining for ten minutes, 37 degrees C in reaction temperature and the salt concentration and ionic strength below 6.0 x 10(-2). In rat and resected human stomachs, gastric mucus was clearly stained under these conditions. In human subjects, the in vivo stained muscu was observed endoscopically. The pyloric gland region. The difference was seen in the pattern of the gastric area between the fundic and pyloric gland region. Histological examination revealed that only the mucous layer was stained with Azure A. The stained macromolecules in the mucus and factors affecting the staining were discussed.", "PMID": 961684}
{"id": "pubmed23n0032_4565", "title": "The role of coloscopy in the differential diagnosis between idiopathic ulcerative colitis and Crohn's disease of the colon.", "content": "The authors point out the striking significance of coloscopy in establishing a correct diagnosis of inflammatory diseases of the colon. In particular, the most valuable endoscopic features are indicated which may permit distinguishing between idiopathic ulcerative colitis and Crohn's disease of the colon. Differentiation between these two diseases cannot always be achieved by means of available diagnostic procedures other than coloscopy. Moreover, the endoscopic findings enable an assessment to be made of the extent, stage, severity and course of either disease. The authors' experience encompass 2,478 coloscopy examinations: the observed cases of idiopathic ulcerative colitis are 182, those of Crohn's disease of the colon are 104.", "contents": "The role of coloscopy in the differential diagnosis between idiopathic ulcerative colitis and Crohn's disease of the colon. The authors point out the striking significance of coloscopy in establishing a correct diagnosis of inflammatory diseases of the colon. In particular, the most valuable endoscopic features are indicated which may permit distinguishing between idiopathic ulcerative colitis and Crohn's disease of the colon. Differentiation between these two diseases cannot always be achieved by means of available diagnostic procedures other than coloscopy. Moreover, the endoscopic findings enable an assessment to be made of the extent, stage, severity and course of either disease. The authors' experience encompass 2,478 coloscopy examinations: the observed cases of idiopathic ulcerative colitis are 182, those of Crohn's disease of the colon are 104.", "PMID": 961685}
{"id": "pubmed23n0032_4566", "title": "Acute yersinial ileitis: a distinct entity.", "content": "A case report of documented Yersinial acute ileitis is presented. A review of the literature reveals that progression to Crohn's disease is uncommon. Yersinosis can present as several symptom complexes. Mesenteric lymphadenitis and terminal ileitis, however, appear to be the most common. The diagnosis is usually made during laparotomy for possible appendicitis, because the symptom complex may be very similar. If the only finding at laparotomy is a thickened edematous ileum, postoperative titers for Yersinia should be taken. A greater awareness is necessary before the true incidence and epidemiology can be ascertained.", "contents": "Acute yersinial ileitis: a distinct entity. A case report of documented Yersinial acute ileitis is presented. A review of the literature reveals that progression to Crohn's disease is uncommon. Yersinosis can present as several symptom complexes. Mesenteric lymphadenitis and terminal ileitis, however, appear to be the most common. The diagnosis is usually made during laparotomy for possible appendicitis, because the symptom complex may be very similar. If the only finding at laparotomy is a thickened edematous ileum, postoperative titers for Yersinia should be taken. A greater awareness is necessary before the true incidence and epidemiology can be ascertained.", "PMID": 961687}
{"id": "pubmed23n0032_4567", "title": "Systemic sclerosis and regional enteritis occurring simultaneously.", "content": "A 58-year-old white female who had regional enteritis for many years insidiously developed scleroderma. A review of the literature failed to reveal any previous reports of this combination of diseases. The possible etiologies of both diseases are discussed along with their overlapping clinical manifestations.", "contents": "Systemic sclerosis and regional enteritis occurring simultaneously. A 58-year-old white female who had regional enteritis for many years insidiously developed scleroderma. A review of the literature failed to reveal any previous reports of this combination of diseases. The possible etiologies of both diseases are discussed along with their overlapping clinical manifestations.", "PMID": 961688}
{"id": "pubmed23n0032_4568", "title": "Acculturation and coronary heart disease in Japanese-Americans.", "content": "Among men of Japanese ancestry, there is a gradient in the occurrence of coronary heart disease (CHD). It is lowest in Japan, intermediate in Hawaii, and highest in California. This gradient appears not to be completely explained by differences in dietary intake, serum cholesterol, blood pressure or smoking. To test the hypothesis that social and cultural differences may account for the CHD differences between Japan and the United States, 3809 Japanese-Americans in California were classified according to the degree to which they retained a traditional Japanese culture. The most traditional group of Japanese-Americans had a CHD prevalence as low as that observed in Japan. The group that was most acculturated to Western culture had a three- to five-fold excess in CHD prevalence. This difference in CHD rate between most and least acculturated groups could not be accounted for by differences in the major coronary risk factors.", "contents": "Acculturation and coronary heart disease in Japanese-Americans. Among men of Japanese ancestry, there is a gradient in the occurrence of coronary heart disease (CHD). It is lowest in Japan, intermediate in Hawaii, and highest in California. This gradient appears not to be completely explained by differences in dietary intake, serum cholesterol, blood pressure or smoking. To test the hypothesis that social and cultural differences may account for the CHD differences between Japan and the United States, 3809 Japanese-Americans in California were classified according to the degree to which they retained a traditional Japanese culture. The most traditional group of Japanese-Americans had a CHD prevalence as low as that observed in Japan. The group that was most acculturated to Western culture had a three- to five-fold excess in CHD prevalence. This difference in CHD rate between most and least acculturated groups could not be accounted for by differences in the major coronary risk factors.", "PMID": 961690}
{"id": "pubmed23n0032_4569", "title": "Influenza detection: a prospective comparison of surveillance methods and analysis of isolates.", "content": "Several prospective methods for surveillance of influenza were compared for the speed and efficacy with which they identified cases of influenza in metropolitan Atlanta during 1972-1975, a period in which two outbreaks of influenza occurred. In a large city-county hospital's outpatient clinics, systematic collection of pharyngeal cultures for virus isolation provided a rapid means for discovering the presence of influenzal illness and for monitoring the extent of the outbreak. Both obtaining cultures and determining the percentage of patients with respiratory complaints indicated an outbreak 3-5 weeks before epidemic disease was confirmed by a rise in the number of deaths due to pneumonia and influenza. Antigenic analysis of influenza A strains isolated during this three-year period showed that influenza A strains recovered in Atlanta in 1973-1974 (a non-epidemic year) were identical to the strains recovered in the 1974-1975 epidemic. Why no epidemic occurred in 1973-1974 in the presence of the epidemic strain of the virus is unknown, and the lack of an epidemic suggests that the role of virus persistence in the epidemiology of influenza requires further evaluation.", "contents": "Influenza detection: a prospective comparison of surveillance methods and analysis of isolates. Several prospective methods for surveillance of influenza were compared for the speed and efficacy with which they identified cases of influenza in metropolitan Atlanta during 1972-1975, a period in which two outbreaks of influenza occurred. In a large city-county hospital's outpatient clinics, systematic collection of pharyngeal cultures for virus isolation provided a rapid means for discovering the presence of influenzal illness and for monitoring the extent of the outbreak. Both obtaining cultures and determining the percentage of patients with respiratory complaints indicated an outbreak 3-5 weeks before epidemic disease was confirmed by a rise in the number of deaths due to pneumonia and influenza. Antigenic analysis of influenza A strains isolated during this three-year period showed that influenza A strains recovered in Atlanta in 1973-1974 (a non-epidemic year) were identical to the strains recovered in the 1974-1975 epidemic. Why no epidemic occurred in 1973-1974 in the presence of the epidemic strain of the virus is unknown, and the lack of an epidemic suggests that the role of virus persistence in the epidemiology of influenza requires further evaluation.", "PMID": 961691}
{"id": "pubmed23n0032_4570", "title": "A study of hepatitis B antigen carriers among schoolchildren in Netanya, Israel.", "content": "In the town of Netanya 9162 elementary schoolchildren (age 6-14 years) were tested for hepatitis B antigen (HBsAg) by the counter-electrophoresis method. The overall prevalence was 1.8% with a higher rate for males (2.2%) than for females (1.4%). Higher rates were observed among children born in the autumn and winter, particularly among the boys. This seasonal trend and male-female difference may partially be explained if infection occurs at circumcision. The ethnic distribution showed highest rates among children of Libyan and Yemenite origin (3.9% and 2.6%, respectively), and lowest rates among children of European and Israeli born parents (0.4% and 0.2%, respectively). Prevalence of HBsAg among children from large families and those from families living in poor and crowded quarters was very significantly higher than among children from smaller families living in richer areas and in less crowded conditions. These variables, which are closely associated with social class, remain significant after adjustment for ethnic origin and seem to account for a large proportion of the variation among the Israeli schoolchildren. The variation due to ethnicity is markedly reduced when adjusted for five other pertinent variables: age, sex, season of birth, crowding and family size. Assuming that prevalence of HBsAg in a population reflects the rates of infection (past or present), the results of this study strongly support the hypothesis that type B hepatitis in Israel behaves essentially as a contagious infection manifested primarily as a subclinical disease of early and mid-childhood.", "contents": "A study of hepatitis B antigen carriers among schoolchildren in Netanya, Israel. In the town of Netanya 9162 elementary schoolchildren (age 6-14 years) were tested for hepatitis B antigen (HBsAg) by the counter-electrophoresis method. The overall prevalence was 1.8% with a higher rate for males (2.2%) than for females (1.4%). Higher rates were observed among children born in the autumn and winter, particularly among the boys. This seasonal trend and male-female difference may partially be explained if infection occurs at circumcision. The ethnic distribution showed highest rates among children of Libyan and Yemenite origin (3.9% and 2.6%, respectively), and lowest rates among children of European and Israeli born parents (0.4% and 0.2%, respectively). Prevalence of HBsAg among children from large families and those from families living in poor and crowded quarters was very significantly higher than among children from smaller families living in richer areas and in less crowded conditions. These variables, which are closely associated with social class, remain significant after adjustment for ethnic origin and seem to account for a large proportion of the variation among the Israeli schoolchildren. The variation due to ethnicity is markedly reduced when adjusted for five other pertinent variables: age, sex, season of birth, crowding and family size. Assuming that prevalence of HBsAg in a population reflects the rates of infection (past or present), the results of this study strongly support the hypothesis that type B hepatitis in Israel behaves essentially as a contagious infection manifested primarily as a subclinical disease of early and mid-childhood.", "PMID": 961692}
{"id": "pubmed23n0032_4571", "title": "Pharyngeal isolations of adenovirus 31 from a family population.", "content": "During a 14-month period in 1965 and 1966, adenovirus type 31 was isolated from 26 pharyngeal swabs obtained on a routine schedule from children and adults during a study of respiratory viruses among members of a family population. Eighteen isolations were made in a four-day period, seven within the preceding five months, and the last, eight months earlier. A significant association between the isolation of adenovirus 31 and respiratory or gastrointestinal illnesses was not demonstrable. Limited tests demonstrated neutralizing antibody increases in a few paired sera of infected adults. The virus is antigenically related to adenoviruses 12 and 18 and is capable of inducing soft tissue tumors in mice.", "contents": "Pharyngeal isolations of adenovirus 31 from a family population. During a 14-month period in 1965 and 1966, adenovirus type 31 was isolated from 26 pharyngeal swabs obtained on a routine schedule from children and adults during a study of respiratory viruses among members of a family population. Eighteen isolations were made in a four-day period, seven within the preceding five months, and the last, eight months earlier. A significant association between the isolation of adenovirus 31 and respiratory or gastrointestinal illnesses was not demonstrable. Limited tests demonstrated neutralizing antibody increases in a few paired sera of infected adults. The virus is antigenically related to adenoviruses 12 and 18 and is capable of inducing soft tissue tumors in mice.", "PMID": 961693}
{"id": "pubmed23n0032_4572", "title": "Nosocomial infection with highly resistant, Proteus rettgeri. Report of an epidemic.", "content": "Over 22-1/2 months an epidemic of at least 127 cases of nosocomial infection developed from a strain of Proteus rettgeri resistant to all antibiotics commonly tested in hospital laboratories. Although there were at least four cases of septicemia and one related death, the majority of cases consisted of asymptomatic bacteriuria or clinically mild urinary tract infection. Indwelling urinary tract devices and antibiotic therapy were important predisposing factors. Data supported an association between increasing use of gentamicin and increasing rates of resistant infection. No common source was found, and contact spread appeared more likely. Control measures included efforts to reduce unnecessary exposure to the incriminated risk factors and to improve asepsis in the management of catheterized patients. An additional 36 cases and one related death were identified in the 7-1/2 months following the investigation and institution of control measures. Nosocomial infection with extremely resistant organisms may pose a serious hazard wherever indwelling urinary tract devices and antibiotics are used together intensively.", "contents": "Nosocomial infection with highly resistant, Proteus rettgeri. Report of an epidemic. Over 22-1/2 months an epidemic of at least 127 cases of nosocomial infection developed from a strain of Proteus rettgeri resistant to all antibiotics commonly tested in hospital laboratories. Although there were at least four cases of septicemia and one related death, the majority of cases consisted of asymptomatic bacteriuria or clinically mild urinary tract infection. Indwelling urinary tract devices and antibiotic therapy were important predisposing factors. Data supported an association between increasing use of gentamicin and increasing rates of resistant infection. No common source was found, and contact spread appeared more likely. Control measures included efforts to reduce unnecessary exposure to the incriminated risk factors and to improve asepsis in the management of catheterized patients. An additional 36 cases and one related death were identified in the 7-1/2 months following the investigation and institution of control measures. Nosocomial infection with extremely resistant organisms may pose a serious hazard wherever indwelling urinary tract devices and antibiotics are used together intensively.", "PMID": 961694}
{"id": "pubmed23n0032_4573", "title": "Distribution of suspected tick vectors and reported cases of Rocky Mountain spotted fever in California.", "content": "Evidence is presented for an area of human risk to Rocky Mountain spotted fever (RMSF) in California extending beyond the range of its classic tick vector, Dermacentor andersoni. Geographic, seasonal, and host distributions of Dermacentor variabilis and Dermacentor occidentalis suggest these species as potential vectors of RMSF in California outside the range of Dermacentor andersoni.", "contents": "Distribution of suspected tick vectors and reported cases of Rocky Mountain spotted fever in California. Evidence is presented for an area of human risk to Rocky Mountain spotted fever (RMSF) in California extending beyond the range of its classic tick vector, Dermacentor andersoni. Geographic, seasonal, and host distributions of Dermacentor variabilis and Dermacentor occidentalis suggest these species as potential vectors of RMSF in California outside the range of Dermacentor andersoni.", "PMID": 961695}
{"id": "pubmed23n0032_4574", "title": "Oral rabies vaccine: evaluation of its infectivity in three species of rodents.", "content": "The safety of oral rabies vaccine was evaluated in 3 species of rodents, animals which might inadvertently consume field-distributed vaccine baits intended for foxes. The potentials for infection from bait ingestion and transmission through cannibalism were evaluated. Results indicate that ingestion of the vaccine baits used in these experiments will result in infection and disease of some rodents. Of 64 rodents which ate baits, 19 (30%) died of clinical rabies; the diagnosis was confirmed in 11 (17%), and 2 (3%) had demonstrable rabies virus in their salivary glands. Only 3 of 112 (3%) rodents that ate vaccine-infected rodents contracted rabies, and none had demonstrable virus in their salivary glands.", "contents": "Oral rabies vaccine: evaluation of its infectivity in three species of rodents. The safety of oral rabies vaccine was evaluated in 3 species of rodents, animals which might inadvertently consume field-distributed vaccine baits intended for foxes. The potentials for infection from bait ingestion and transmission through cannibalism were evaluated. Results indicate that ingestion of the vaccine baits used in these experiments will result in infection and disease of some rodents. Of 64 rodents which ate baits, 19 (30%) died of clinical rabies; the diagnosis was confirmed in 11 (17%), and 2 (3%) had demonstrable rabies virus in their salivary glands. Only 3 of 112 (3%) rodents that ate vaccine-infected rodents contracted rabies, and none had demonstrable virus in their salivary glands.", "PMID": 961696}
{"id": "pubmed23n0032_4575", "title": "Effect of immunosuppressants on dogs exposed two and one-half years previously to Blastomyces dermatitidis.", "content": "Observation of 33 dogs exposed to Blastomyces dermatitidis over a  2 1/2 year period is reported. During the first 1 1/2 years six dogs died and 23 (77%) developed skin lesions. Clinical evidence of disease in all the animals then subsided and remained so through another year of observation. Nine animals were sacrificed after 120 weeks and several tissues were cultured from each one. The remaining 18 were given immunosuppressants--azathioprine, prednisone, and cyclophosphamide--for eight weeks prior to autopsy. About one-half of the dogs were found to be infected with Histoplasma capsulatum and all were negative for B. dermatitidis by culture. The dogs were thought to have been inadvertently exposed to H. capsulatum during the first eight weeks of observation from a nearby soil plot before being moved to a more remote shelter. Skin testing with histoplasmin and blastomycin and complement-fixation (CF) tests, performed at various intervals after exposure to B. dermatitidis, appeared to reflect the course of disease with the number of reactors to the H. capsulatum antigens remaining relatively high while the number of reactors to B. dermatitidis antigens fell with the passage of time. The blastomycin skin test results in these dogs, however, persisted far longer than positive Blastomyces CF results. These data suggest that most dogs exposed to B. dermatitidis exhibit clinical symptoms of disease after about 12 weeks, persisting for about one year. The majority of animals recover spontaneously and completely. With H. capsulatum, however, the infection can be subclinical for at least a two-year period.", "contents": "Effect of immunosuppressants on dogs exposed two and one-half years previously to Blastomyces dermatitidis. Observation of 33 dogs exposed to Blastomyces dermatitidis over a  2 1/2 year period is reported. During the first 1 1/2 years six dogs died and 23 (77%) developed skin lesions. Clinical evidence of disease in all the animals then subsided and remained so through another year of observation. Nine animals were sacrificed after 120 weeks and several tissues were cultured from each one. The remaining 18 were given immunosuppressants--azathioprine, prednisone, and cyclophosphamide--for eight weeks prior to autopsy. About one-half of the dogs were found to be infected with Histoplasma capsulatum and all were negative for B. dermatitidis by culture. The dogs were thought to have been inadvertently exposed to H. capsulatum during the first eight weeks of observation from a nearby soil plot before being moved to a more remote shelter. Skin testing with histoplasmin and blastomycin and complement-fixation (CF) tests, performed at various intervals after exposure to B. dermatitidis, appeared to reflect the course of disease with the number of reactors to the H. capsulatum antigens remaining relatively high while the number of reactors to B. dermatitidis antigens fell with the passage of time. The blastomycin skin test results in these dogs, however, persisted far longer than positive Blastomyces CF results. These data suggest that most dogs exposed to B. dermatitidis exhibit clinical symptoms of disease after about 12 weeks, persisting for about one year. The majority of animals recover spontaneously and completely. With H. capsulatum, however, the infection can be subclinical for at least a two-year period.", "PMID": 961697}
{"id": "pubmed23n0032_4576", "title": "Sepsis associated with decubitus ulcers.", "content": "Among 21 patients with sepsis attributed solely to decubitus ulcers, bacteremia was documented in 16 (76 per cent)9 Bacteremia involved obligate anaerobes in eight patients (50 per cent) and was polymicrobial in eight patients (50 per cent). Twelve of 17 patients who received antibiotics had persistent bacteremia; in five patients, bacteremia was terminated only after surgical debridement. Ten of these 21 patients died, eight despite appropirate antibiotics. Among 14 patients who underwent surgical debridement, only four patients died. Surgical debridement and antibiotics effective against aerobic as well as anaerobic bacteria are both important in the treatment of this serious complication.", "contents": "Sepsis associated with decubitus ulcers. Among 21 patients with sepsis attributed solely to decubitus ulcers, bacteremia was documented in 16 (76 per cent)9 Bacteremia involved obligate anaerobes in eight patients (50 per cent) and was polymicrobial in eight patients (50 per cent). Twelve of 17 patients who received antibiotics had persistent bacteremia; in five patients, bacteremia was terminated only after surgical debridement. Ten of these 21 patients died, eight despite appropirate antibiotics. Among 14 patients who underwent surgical debridement, only four patients died. Surgical debridement and antibiotics effective against aerobic as well as anaerobic bacteria are both important in the treatment of this serious complication.", "PMID": 961699}
{"id": "pubmed23n0032_4577", "title": "The cardiac conduction system in progressive systemic sclerosis. Clinical and pathologic features of 35 patients.", "content": "Progressive systemic sclerosis may be associated with focal myocardial fibrosis. Electrocardiographic abnormalities including conduction block are common in progressive systemic sclerosis but whether they are due to direct destruction of the specialized conduction tissue of the heart is uncertain. The conduction systems of 35 patients with progressive systemic sclerosis were studied. Of these 35 patients, 17 (50 per cent) had myocardial fibrosis of the type seen in progressive systemic sclerosis. In 10 of the 17, it was severe. Sinus node fibrosis was present in 13 patients and was nearly as frequent in those with as in those without the progressive systemic sclerosis myocardial lesion. Overlying pericarditis may have contributed to the fibrotic changes within the sinoatrial nodes in seven of the 13 patients. The atrioventricular node and main His bundles were normal. However, fibrotic changes were found in the proximal bundle systems in six patients. In three of the six, severe myocardial progressive systemic sclerosis was present, two had focal fibrous atrophy of the left bundle, and one had complete interruption of the right bundle. In only the latter patient was this reflected in the electrocardiogram which showed a right bundle branch block. Three patients without progressive systemic sclerosis myocardial lesions also had fibrous atrophy of a portion of the proximal left bundle branch, and in one the electrocardiogram showed an isolated left anterior hemiblock. Thus, morphologic abnormalities within the conduction system in our patients are difficult to attribute to progressive systemic sclerosis per se. Furthermore, although conduction abnormalities were more frequent in patients with myocardial disease, specific conduction system disease was not the cause in most patients. As has been noted in ischemic heart disease, the conduction system appears to be relatively spared from the myocardial changes of progressive systemic sclerosis, and the high incidence of conduction disturbances in this condition may be a consequence, rather, of damage to working myocardium.", "contents": "The cardiac conduction system in progressive systemic sclerosis. Clinical and pathologic features of 35 patients. Progressive systemic sclerosis may be associated with focal myocardial fibrosis. Electrocardiographic abnormalities including conduction block are common in progressive systemic sclerosis but whether they are due to direct destruction of the specialized conduction tissue of the heart is uncertain. The conduction systems of 35 patients with progressive systemic sclerosis were studied. Of these 35 patients, 17 (50 per cent) had myocardial fibrosis of the type seen in progressive systemic sclerosis. In 10 of the 17, it was severe. Sinus node fibrosis was present in 13 patients and was nearly as frequent in those with as in those without the progressive systemic sclerosis myocardial lesion. Overlying pericarditis may have contributed to the fibrotic changes within the sinoatrial nodes in seven of the 13 patients. The atrioventricular node and main His bundles were normal. However, fibrotic changes were found in the proximal bundle systems in six patients. In three of the six, severe myocardial progressive systemic sclerosis was present, two had focal fibrous atrophy of the left bundle, and one had complete interruption of the right bundle. In only the latter patient was this reflected in the electrocardiogram which showed a right bundle branch block. Three patients without progressive systemic sclerosis myocardial lesions also had fibrous atrophy of a portion of the proximal left bundle branch, and in one the electrocardiogram showed an isolated left anterior hemiblock. Thus, morphologic abnormalities within the conduction system in our patients are difficult to attribute to progressive systemic sclerosis per se. Furthermore, although conduction abnormalities were more frequent in patients with myocardial disease, specific conduction system disease was not the cause in most patients. As has been noted in ischemic heart disease, the conduction system appears to be relatively spared from the myocardial changes of progressive systemic sclerosis, and the high incidence of conduction disturbances in this condition may be a consequence, rather, of damage to working myocardium.", "PMID": 961700}
{"id": "pubmed23n0032_4578", "title": "Immunodeficiency, xanthomas and obstructive liver disease.", "content": "Chronic obstructive liver disease and secondary hyperlipidemia developed in an immunodeficient boy. Sequential addition of cholestyramine and phenobarbital to his medical regimen, following an initial response to bile drainage, resulted in the disappearance of xanthomas and pruritus, and the restoration of normal serum concentrations of lipids and bile acids. This improvement may result from shifting the bile acid pool from the peripheral blood compartment to the enterohepatic circulation.", "contents": "Immunodeficiency, xanthomas and obstructive liver disease. Chronic obstructive liver disease and secondary hyperlipidemia developed in an immunodeficient boy. Sequential addition of cholestyramine and phenobarbital to his medical regimen, following an initial response to bile drainage, resulted in the disappearance of xanthomas and pruritus, and the restoration of normal serum concentrations of lipids and bile acids. This improvement may result from shifting the bile acid pool from the peripheral blood compartment to the enterohepatic circulation.", "PMID": 961702}
{"id": "pubmed23n0032_4579", "title": "Goodpasture's syndrome in a patient with the Nail-Patella syndrome.", "content": "A patient with the nail-patella syndrome in whom end-stage renal failuure developed as the result of Goodpasture's syndrome is described. Lesions characteristic of both rare diseases were seen on renal morphology. It is postulated that the glomerular membrane alteration of the nail-patella syndrome predisposed to the development of antiglomerular basement membrane antibody and hence Goodpasture's syndrome. A review of the incidence of renal failure in the nail-patella syndrome suggests that renal involvement can no longer be regarded as benign and that immune mechanisms may be related to progressive renal disease in some cases.", "contents": "Goodpasture's syndrome in a patient with the Nail-Patella syndrome. A patient with the nail-patella syndrome in whom end-stage renal failuure developed as the result of Goodpasture's syndrome is described. Lesions characteristic of both rare diseases were seen on renal morphology. It is postulated that the glomerular membrane alteration of the nail-patella syndrome predisposed to the development of antiglomerular basement membrane antibody and hence Goodpasture's syndrome. A review of the incidence of renal failure in the nail-patella syndrome suggests that renal involvement can no longer be regarded as benign and that immune mechanisms may be related to progressive renal disease in some cases.", "PMID": 961703}
{"id": "pubmed23n0032_4580", "title": "The appearance of nonlinear deposits of immunoglobulins in Goodpasture's syndrome.", "content": "A twenty year old woman presented with pulmonary hemorrhage and glomerulonephritis with linear deposition of IgG on the glomerular capillary basement membrane, and high titer of antiglomerular basement membrane antibody in her serum. Without treatment, renal function, which was transiently impaired, has improved, pulmonary hemorrhage subsided, and repeat renal biopsies have revealed the appearance of nonlinear in addition to the linear deposition of IgG on the glomerular capillary basement membrane in the face of diminished serum antiglomerular basement membrane antibody titer.", "contents": "The appearance of nonlinear deposits of immunoglobulins in Goodpasture's syndrome. A twenty year old woman presented with pulmonary hemorrhage and glomerulonephritis with linear deposition of IgG on the glomerular capillary basement membrane, and high titer of antiglomerular basement membrane antibody in her serum. Without treatment, renal function, which was transiently impaired, has improved, pulmonary hemorrhage subsided, and repeat renal biopsies have revealed the appearance of nonlinear in addition to the linear deposition of IgG on the glomerular capillary basement membrane in the face of diminished serum antiglomerular basement membrane antibody titer.", "PMID": 961704}
{"id": "pubmed23n0032_4581", "title": "Splenosis: autotransplantation of splenic tissue.", "content": "Splenosis is the autotransplantation of splenic tissue that usually follows traumatic rupture of the spleen. Generally, the splenic implants are numerous and are located within the peritoneal cavity; however, extra-abdominal splenosis does occur. The implants are rarely clinically significant and are incidental findings at autopsy or at abdominal operation, unrelated to and distant from the splenic trauma and splenectomy. Splenic implants retain their ability to function, and recurrence of a hematologic disease for which the spleen was previously removed should alter the  clinician to the possibility of splenosis. The differential diagnosis includes accessory spleens, endometriosis, hemangiomas and metastatic cancer. We report three cases, two of which represent unusual complications of splenosis: a twisted pedicle of a splenic implant, which apparently caused abdominal pain, and recurrent Felty's syndrome associated with splenosis and an accessory spleen.", "contents": "Splenosis: autotransplantation of splenic tissue. Splenosis is the autotransplantation of splenic tissue that usually follows traumatic rupture of the spleen. Generally, the splenic implants are numerous and are located within the peritoneal cavity; however, extra-abdominal splenosis does occur. The implants are rarely clinically significant and are incidental findings at autopsy or at abdominal operation, unrelated to and distant from the splenic trauma and splenectomy. Splenic implants retain their ability to function, and recurrence of a hematologic disease for which the spleen was previously removed should alter the  clinician to the possibility of splenosis. The differential diagnosis includes accessory spleens, endometriosis, hemangiomas and metastatic cancer. We report three cases, two of which represent unusual complications of splenosis: a twisted pedicle of a splenic implant, which apparently caused abdominal pain, and recurrent Felty's syndrome associated with splenosis and an accessory spleen.", "PMID": 961705}
{"id": "pubmed23n0032_4582", "title": "Regression of atherosclerotic stenosing lesions of the renal arteries and spontaneous cure of systemic hypertension through control of hyperlipidemia.", "content": "We describe a 49 year old woman with impressive regression of renal artery stenosing atherosclerotic lesions in response to sustained excellent control of hyperlipidemia. Initially, she had high blood pressure necessitating combined drug therapy with chlorothiazide, methyldopa and propranolol, with only moderately satisfactory control. Renal arteriography revealed a 90 per cent stenosing lesion of the right renal artery and 75 per cent narrowing of the left renal artery. Peripheral vein plasma renin was markedly increased at 32 ng/ml. With a combination of cholestyramine and clofibrate, serum lipids were maintained at normal values for several years. Increased blood pressure diminished spontaneously, and the patient has maintained normal blood pressures after discontinuation of antihypertensive therapy. Repeat renal arteriograms showed almost complete regression of the right renal artery lesion and a possible decrease in left renal artery disease. Peripheral vein plasma renin became normal at 3 ng/ml. This case illustrates that sustained control of hyperlipidemia could lead to regression of atherosclerotic nodules and impressive clinical improvement in certain patients.", "contents": "Regression of atherosclerotic stenosing lesions of the renal arteries and spontaneous cure of systemic hypertension through control of hyperlipidemia. We describe a 49 year old woman with impressive regression of renal artery stenosing atherosclerotic lesions in response to sustained excellent control of hyperlipidemia. Initially, she had high blood pressure necessitating combined drug therapy with chlorothiazide, methyldopa and propranolol, with only moderately satisfactory control. Renal arteriography revealed a 90 per cent stenosing lesion of the right renal artery and 75 per cent narrowing of the left renal artery. Peripheral vein plasma renin was markedly increased at 32 ng/ml. With a combination of cholestyramine and clofibrate, serum lipids were maintained at normal values for several years. Increased blood pressure diminished spontaneously, and the patient has maintained normal blood pressures after discontinuation of antihypertensive therapy. Repeat renal arteriograms showed almost complete regression of the right renal artery lesion and a possible decrease in left renal artery disease. Peripheral vein plasma renin became normal at 3 ng/ml. This case illustrates that sustained control of hyperlipidemia could lead to regression of atherosclerotic nodules and impressive clinical improvement in certain patients.", "PMID": 961706}
{"id": "pubmed23n0032_4583", "title": "Arthritis with an inflammatory dermatosis resembling Sweet's syndrome. Report of a unique case and review of the literature on arthritis associated with the inflammatory dermatoses.", "content": "A patient with a unique case of chronic episodic arthritis coincident with flares of acneform, pustular, nodular and ulcerating skin lesions was observed over a five-year period. This patient and a review of the literature on arthritis associated with the inflammatory dermatoses provide evidence which may interrelate several of these nosologically confusing skin conditions, e.g., the family of leukocytoclastic angiitides with the newly posited acute febrile neutrophilic dermatosis of Sweet. Systemic manifestations and a variety of acneform, pustular, nodular and ulcerating cutaneous lesions in the inflammatory dermatoses are best explained by small vessel involvement, with individual syndromes being determined by the type and degree of vascular change. Perivascular neutrophilic infiltration is the unifying histologic feature of these small vessel diseases. Neutrophil infiltration differentiates these entities, and our patient, from the histologically nonspecific inflammations of the skin, e.g., Behcet's disease and pyoderma gangrenosum, which, although capable of causing identically appearing skin lesions, consist predominantly of lymphocytic dermal infiltrates even in the earlier stages. It appears important to recognized these morphologically varied acute inflammatory dermatoses with perivascular neutrophilic infiltration in view of their systemic features and the dramatic efficacy of corticosteroid therapy.", "contents": "Arthritis with an inflammatory dermatosis resembling Sweet's syndrome. Report of a unique case and review of the literature on arthritis associated with the inflammatory dermatoses. A patient with a unique case of chronic episodic arthritis coincident with flares of acneform, pustular, nodular and ulcerating skin lesions was observed over a five-year period. This patient and a review of the literature on arthritis associated with the inflammatory dermatoses provide evidence which may interrelate several of these nosologically confusing skin conditions, e.g., the family of leukocytoclastic angiitides with the newly posited acute febrile neutrophilic dermatosis of Sweet. Systemic manifestations and a variety of acneform, pustular, nodular and ulcerating cutaneous lesions in the inflammatory dermatoses are best explained by small vessel involvement, with individual syndromes being determined by the type and degree of vascular change. Perivascular neutrophilic infiltration is the unifying histologic feature of these small vessel diseases. Neutrophil infiltration differentiates these entities, and our patient, from the histologically nonspecific inflammations of the skin, e.g., Behcet's disease and pyoderma gangrenosum, which, although capable of causing identically appearing skin lesions, consist predominantly of lymphocytic dermal infiltrates even in the earlier stages. It appears important to recognized these morphologically varied acute inflammatory dermatoses with perivascular neutrophilic infiltration in view of their systemic features and the dramatic efficacy of corticosteroid therapy.", "PMID": 961707}
{"id": "pubmed23n0032_4584", "title": "Absence of imune deficiencies in a case of progressive multifocal leukoencephalopathy.", "content": "Progressive multifocal leukoencephalopathy (PML) is a rare disease related to a slow virus infection of the central nervous system; it is usually seen in patients who have impaired immunologic function. The present patient with biopsy-proved PML was found to have no demonstrable defects in either cellular or humoral immunity as assessed by multiple parameters. Thus, it appears that PML may occur in the presence of intact immune responses.", "contents": "Absence of imune deficiencies in a case of progressive multifocal leukoencephalopathy. Progressive multifocal leukoencephalopathy (PML) is a rare disease related to a slow virus infection of the central nervous system; it is usually seen in patients who have impaired immunologic function. The present patient with biopsy-proved PML was found to have no demonstrable defects in either cellular or humoral immunity as assessed by multiple parameters. Thus, it appears that PML may occur in the presence of intact immune responses.", "PMID": 961708}
{"id": "pubmed23n0032_4585", "title": "Sickle cell--betao thalassemia variant with high hemoglobin F and mild clinical course.", "content": "A 70 year old Black woman had chronic hemolytic anemia without recurrent painful crises. Hemoglobin pattern by electrophoresis was hemoglobin S (69 to 71 per cent), hemoglobin A2 (4.6 per cent) and hemoglobin F (24 to 27 per cent). No hemoglobin A was detected, and the hemoglobin F was distributed heterogeneously in the red cells. Reticulocyte alpha/nonalpha globin chain synthetic ratios were 1.44 to 1.62. Thus, the patient had a high hemoglobin F variant of S-beta zero (betao) thalassemia which has not been described previously. Her clinical course has been mild in comparison with S-betao thalassemia patients who do not have extremely elevated hemoglobin F levels.", "contents": "Sickle cell--betao thalassemia variant with high hemoglobin F and mild clinical course. A 70 year old Black woman had chronic hemolytic anemia without recurrent painful crises. Hemoglobin pattern by electrophoresis was hemoglobin S (69 to 71 per cent), hemoglobin A2 (4.6 per cent) and hemoglobin F (24 to 27 per cent). No hemoglobin A was detected, and the hemoglobin F was distributed heterogeneously in the red cells. Reticulocyte alpha/nonalpha globin chain synthetic ratios were 1.44 to 1.62. Thus, the patient had a high hemoglobin F variant of S-beta zero (betao) thalassemia which has not been described previously. Her clinical course has been mild in comparison with S-betao thalassemia patients who do not have extremely elevated hemoglobin F levels.", "PMID": 961709}
{"id": "pubmed23n0032_4586", "title": "Enterobacteriaceae identification compared by MORLOC (a new system) and API 20E.", "content": "Identifications of 201 Enterobacteriaceae isolates were compared by MORLOC (a new system) and the API 20E system. Identifications agreed for 192 isolates (95.5 per cent). Of the nine discrepancies, MORLOC was correct for four, API for three; neither for one; and for one, MORLOC agreed with the reference laboratory but apparently all three systems were incorrect. The MORLOC system requires an extra day for a TSI but is smaller and simpler and can be recommended as easier to use, more reproducible, less expensive, and, in this study, more accurate than the API 20E.", "contents": "Enterobacteriaceae identification compared by MORLOC (a new system) and API 20E. Identifications of 201 Enterobacteriaceae isolates were compared by MORLOC (a new system) and the API 20E system. Identifications agreed for 192 isolates (95.5 per cent). Of the nine discrepancies, MORLOC was correct for four, API for three; neither for one; and for one, MORLOC agreed with the reference laboratory but apparently all three systems were incorrect. The MORLOC system requires an extra day for a TSI but is smaller and simpler and can be recommended as easier to use, more reproducible, less expensive, and, in this study, more accurate than the API 20E.", "PMID": 961710}
{"id": "pubmed23n0032_4587", "title": "Experimental studies on cataract.", "content": "I have discussed five aspects of lens metabolism and their possible relationship to cataract in man, and this has left me with five fundamental questions to be answered. 1. Are the fluorescent tryptophan derivatives, found only in the lens of man and higher primates, involved in the development of brown nuclear cataract? 2. Is naphthalene cataract in rabbits a model for any type of cataract in man--i.e., are quinones ever formed in the human eye? 3. Is diabetes the only cataract in which osmotic swelling is important? 4. Does self-digestion of protein in the human lens contribute to cataract development? 5. Are the consequences of the abnormal maturation of lens fibers, which occurs in tryptophan deficiency cataract in rats, ever seen in man?", "contents": "Experimental studies on cataract. I have discussed five aspects of lens metabolism and their possible relationship to cataract in man, and this has left me with five fundamental questions to be answered. 1. Are the fluorescent tryptophan derivatives, found only in the lens of man and higher primates, involved in the development of brown nuclear cataract? 2. Is naphthalene cataract in rabbits a model for any type of cataract in man--i.e., are quinones ever formed in the human eye? 3. Is diabetes the only cataract in which osmotic swelling is important? 4. Does self-digestion of protein in the human lens contribute to cataract development? 5. Are the consequences of the abnormal maturation of lens fibers, which occurs in tryptophan deficiency cataract in rats, ever seen in man?", "PMID": 961716}
{"id": "pubmed23n0032_4588", "title": "Hyperkalemia: diagnosis and treatment.", "content": "We have considered a differential diagnosis of clinical hyperkalemia. The clinical signs and symptoms of patients with hyperkalemia are manifold and can involve many organ systems. The nonoliguric chronic renal failure patient with hyporeninemia and hypoaldosteronemia may have as one of his principal problems recurrent hyperkalemia. Treatment of this condition includes well-known modalities such as administration of calcium salts, NaHCO3, removal of potassium with resin or dialysis as well as placement of a transvenous pacemaker in anticipation of bradyarrhythmias or asystole.", "contents": "Hyperkalemia: diagnosis and treatment. We have considered a differential diagnosis of clinical hyperkalemia. The clinical signs and symptoms of patients with hyperkalemia are manifold and can involve many organ systems. The nonoliguric chronic renal failure patient with hyporeninemia and hypoaldosteronemia may have as one of his principal problems recurrent hyperkalemia. Treatment of this condition includes well-known modalities such as administration of calcium salts, NaHCO3, removal of potassium with resin or dialysis as well as placement of a transvenous pacemaker in anticipation of bradyarrhythmias or asystole.", "PMID": 961713}
{"id": "pubmed23n0032_4589", "title": "Primary retinal degeneration: evidence of normal phagocytosis in the retinal pigment epithelium.", "content": "In rats with primary retinal degeneration, lens extraction combined with total retinal detachment provided a model for injection of a tracer of colloidal carbon into the subretinal space. Electron microscopy and acid phosphatase cytochemistry were subsequently used to analyze the ingestion of tracer by the retinal pigment epithelium. It was found that the attachment, ingestion, and digestion phases of the phagocytic process were apparently preserved. From this evidence it is suggested that there is no lack of phagocytic power in the retinal pigment epithelium of affected rat strains.", "contents": "Primary retinal degeneration: evidence of normal phagocytosis in the retinal pigment epithelium. In rats with primary retinal degeneration, lens extraction combined with total retinal detachment provided a model for injection of a tracer of colloidal carbon into the subretinal space. Electron microscopy and acid phosphatase cytochemistry were subsequently used to analyze the ingestion of tracer by the retinal pigment epithelium. It was found that the attachment, ingestion, and digestion phases of the phagocytic process were apparently preserved. From this evidence it is suggested that there is no lack of phagocytic power in the retinal pigment epithelium of affected rat strains.", "PMID": 961717}
{"id": "pubmed23n0032_4590", "title": "Sodium and water: an overview.", "content": "The renal regulation of sodium is intertwined with the extracellular fluid volume (ECFV). Most adjustments in sodium elimination in man are accomplished via alterations in tubular reabsorption. The latter is sensitive to change in ECFV. An expanded ECFV results in less reabsorption and more excretion of sodium, and a contracted ECFV has the converse effect. There are direct and indirect mechanisms whereby ECFV influences sodium reabsorption. Patients with nephrotic syndrome, heart failure, and cirrhosis \"behave\" physiologically as normal individuals with a contracted ECFV. Water balance is normally determined by intake and losses in sweat which is always hypoosmotic to plasma, by evaporation from skin and lungs, and through renal excretion. The major factors that determine the ability to concentrate the urine are (1) the establishment of a concentrated environment around the collecting ducts, and (2) the elaboration and effects on the kidney of antidiuretic hormone. The evaluation of a patient with abnormalities of sodium and water rests initially and largely on clinical information. The clinical setting provides clues to anticipating, preventing, and interpreting distortions of body sodium and water. The laboratory can detect an abnormality, confirm or refute clinical assessment, and assist in the quantitative aspects of treatment and its efficacy.", "contents": "Sodium and water: an overview. The renal regulation of sodium is intertwined with the extracellular fluid volume (ECFV). Most adjustments in sodium elimination in man are accomplished via alterations in tubular reabsorption. The latter is sensitive to change in ECFV. An expanded ECFV results in less reabsorption and more excretion of sodium, and a contracted ECFV has the converse effect. There are direct and indirect mechanisms whereby ECFV influences sodium reabsorption. Patients with nephrotic syndrome, heart failure, and cirrhosis \"behave\" physiologically as normal individuals with a contracted ECFV. Water balance is normally determined by intake and losses in sweat which is always hypoosmotic to plasma, by evaporation from skin and lungs, and through renal excretion. The major factors that determine the ability to concentrate the urine are (1) the establishment of a concentrated environment around the collecting ducts, and (2) the elaboration and effects on the kidney of antidiuretic hormone. The evaluation of a patient with abnormalities of sodium and water rests initially and largely on clinical information. The clinical setting provides clues to anticipating, preventing, and interpreting distortions of body sodium and water. The laboratory can detect an abnormality, confirm or refute clinical assessment, and assist in the quantitative aspects of treatment and its efficacy.", "PMID": 961714}
{"id": "pubmed23n0032_4591", "title": "Fluid and electrolyte disturbances in cirrhosis.", "content": "Glomerular filtration rate and renal plasma flow may be normal, reduced or increased in cirrhosis. The mechanism of departures from normal is not known. Other renal functional changes in cirrhosis include avid sodium reabsorption, impaired concentrating and diluting abilities, and partial renal tubular acidosis. Fluid and electrolyte disorders are common. Sodium retention with edema and ascites should generally be treated conservatively because they tend to disappear as the liver heals and because forced diuresis has hazards. The indications for diuretics are (1) incipient or overt atelectasis; (2) abdominal distress; and (3) possibility of skin breakdown. Hyponatremia is common and its mechanism and treatment must be assessed in each patient. Hypokalemia occurs and requires treatment. Respiratory alkalosis and renal tubular acidosis seldom need therapy. The hepatorenal syndrome is defined as functional renal failure in the absence of other known causes of renal functional impairment. The prognosis is terrible and therapy is unsatisfactory. The best approach is not to equate the occurrence of renal failure in cirrhosis with the hepatorenal syndrome. Rather the physician should first explore all treatable causes of renal failure, eg, dehydration, obstruction, infection, heart failure, potassium depletion, and others.", "contents": "Fluid and electrolyte disturbances in cirrhosis. Glomerular filtration rate and renal plasma flow may be normal, reduced or increased in cirrhosis. The mechanism of departures from normal is not known. Other renal functional changes in cirrhosis include avid sodium reabsorption, impaired concentrating and diluting abilities, and partial renal tubular acidosis. Fluid and electrolyte disorders are common. Sodium retention with edema and ascites should generally be treated conservatively because they tend to disappear as the liver heals and because forced diuresis has hazards. The indications for diuretics are (1) incipient or overt atelectasis; (2) abdominal distress; and (3) possibility of skin breakdown. Hyponatremia is common and its mechanism and treatment must be assessed in each patient. Hypokalemia occurs and requires treatment. Respiratory alkalosis and renal tubular acidosis seldom need therapy. The hepatorenal syndrome is defined as functional renal failure in the absence of other known causes of renal functional impairment. The prognosis is terrible and therapy is unsatisfactory. The best approach is not to equate the occurrence of renal failure in cirrhosis with the hepatorenal syndrome. Rather the physician should first explore all treatable causes of renal failure, eg, dehydration, obstruction, infection, heart failure, potassium depletion, and others.", "PMID": 961715}
{"id": "pubmed23n0032_4592", "title": "Histopathology of experimental ethambutol intoxication.", "content": "Ethambutol was administered to albino rats in their drinking water in doses of 105 to 2,500 mg. per kilogram per day for 18 to 102 days. Sixteen per cent developed bilateral lesions consisting of focal axonal swelling without demyelination, in their optic chiasms and the intracranial portions of their optic nerves.", "contents": "Histopathology of experimental ethambutol intoxication. Ethambutol was administered to albino rats in their drinking water in doses of 105 to 2,500 mg. per kilogram per day for 18 to 102 days. Sixteen per cent developed bilateral lesions consisting of focal axonal swelling without demyelination, in their optic chiasms and the intracranial portions of their optic nerves.", "PMID": 961718}
{"id": "pubmed23n0032_4593", "title": "Effects of mechanical agitation on endothelial function of preserved corneas.", "content": "Corneas from adult albino New Zealand rabbits were stored in either MK medium or moist chambers at 4 degrees C. for 18 to 24 hours. During that time, half the corneas from each group were agitated for 8 hours to simulate the motion that might occur during long-distance shipment. The corneas were then placed in specular microscopes and perfused for 4 hours with a modified Krebs-Ringer solution at 34 degrees C. Serial measurements of corneal thickness made during temperature reversal showed that agitation had no effect on endothelial viability of corneas stored in either solution. Electron microscopy of selected corneas confirmed this finding.", "contents": "Effects of mechanical agitation on endothelial function of preserved corneas. Corneas from adult albino New Zealand rabbits were stored in either MK medium or moist chambers at 4 degrees C. for 18 to 24 hours. During that time, half the corneas from each group were agitated for 8 hours to simulate the motion that might occur during long-distance shipment. The corneas were then placed in specular microscopes and perfused for 4 hours with a modified Krebs-Ringer solution at 34 degrees C. Serial measurements of corneal thickness made during temperature reversal showed that agitation had no effect on endothelial viability of corneas stored in either solution. Electron microscopy of selected corneas confirmed this finding.", "PMID": 961719}
{"id": "pubmed23n0032_4594", "title": "Corneal water and electrolyte content following storage in moist chamber and MK medium.", "content": "Rabbit corneas were subjected to MK-medium or moist-chamber storage for periods up to 7 days. Sodium, potassium, and chloride concentrations and hydration were measured. The hydration of corneas stored in MK medium was less than that of those stored in moist chambers. The sodium concentration of MK corneas remained stable for up to 7 days; the potassium concentration, however, decreased with increasingly longer periods of storage. Chloride concentrations quickly equilibrated with the high chloride level of MK medium. The concentration of sodium and chloride in moist chamber-stored corneas fell with progressively longer storage periods. Potassium showed an initial decrease in concentration followed by an increase in concentration that paralleled, and equilibrated with, the increasing acqueous humor concentration.", "contents": "Corneal water and electrolyte content following storage in moist chamber and MK medium. Rabbit corneas were subjected to MK-medium or moist-chamber storage for periods up to 7 days. Sodium, potassium, and chloride concentrations and hydration were measured. The hydration of corneas stored in MK medium was less than that of those stored in moist chambers. The sodium concentration of MK corneas remained stable for up to 7 days; the potassium concentration, however, decreased with increasingly longer periods of storage. Chloride concentrations quickly equilibrated with the high chloride level of MK medium. The concentration of sodium and chloride in moist chamber-stored corneas fell with progressively longer storage periods. Potassium showed an initial decrease in concentration followed by an increase in concentration that paralleled, and equilibrated with, the increasing acqueous humor concentration.", "PMID": 961720}
{"id": "pubmed23n0032_4595", "title": "An improved model of experimentally induced ocular hypertension in the rabbit.", "content": "A model of experimentally induced ocular hypertension for the evaluation of antiglaucoma drugs in the unanesthetized rabbit is described. It is based on the intravenous infusion of suitable amounts of 5 per cent glucose solution, and advantageously substitutes the oral water load. The method is sensitive to drugs acting both on the outflow facility (2 per cent pilocarpine) and an aqueous humor formation (10 per cent guanethidine).", "contents": "An improved model of experimentally induced ocular hypertension in the rabbit. A model of experimentally induced ocular hypertension for the evaluation of antiglaucoma drugs in the unanesthetized rabbit is described. It is based on the intravenous infusion of suitable amounts of 5 per cent glucose solution, and advantageously substitutes the oral water load. The method is sensitive to drugs acting both on the outflow facility (2 per cent pilocarpine) and an aqueous humor formation (10 per cent guanethidine).", "PMID": 961721}
{"id": "pubmed23n0032_4596", "title": "The WISC may not be a valid predictor of school performance for primary-grade minority children.", "content": "In the present investigation we studied the validity of the Wechsler Intelligence Scale for Children (WISC) for predicting several criterial teachers' ratings for primary-grade black, Anglo, and Mexican-American children. The results were rather striking: although validities for the combined groups were good (rs near .6), the validities calculated within each group presented a different picture. Validities were good for the Anglo children but near zero for the black and Mexican-American children. These results suggest that the WISC may be of little value in the assessment of the educability of minority children. The implications of these results for educational placement and the heredity-environment controversy were discussed.", "contents": "The WISC may not be a valid predictor of school performance for primary-grade minority children. In the present investigation we studied the validity of the Wechsler Intelligence Scale for Children (WISC) for predicting several criterial teachers' ratings for primary-grade black, Anglo, and Mexican-American children. The results were rather striking: although validities for the combined groups were good (rs near .6), the validities calculated within each group presented a different picture. Validities were good for the Anglo children but near zero for the black and Mexican-American children. These results suggest that the WISC may be of little value in the assessment of the educability of minority children. The implications of these results for educational placement and the heredity-environment controversy were discussed.", "PMID": 961723}
{"id": "pubmed23n0032_4597", "title": "Transfer of categorial clustering set in mildly retarded adolescents.", "content": "The relative effectiveness of instructions to adopt an organizational strategy vs. exposure to category labels was investigated. Twenty-four retarded adolescents were assigned to each of three conditions (blocking plus instructions, blocking plus labels, and blocking) for pretraining and training. Immediately after training, all subjects received a transfer task consisting of a new randomly organized list containing words from the same (training) categories for one-half of the subjects and from different categories for the other half. When exposure to category labels accompanied blocking, results indicated increased clustering and recall during the training phase and a trend toward increased clustering during the transfer phase.", "contents": "Transfer of categorial clustering set in mildly retarded adolescents. The relative effectiveness of instructions to adopt an organizational strategy vs. exposure to category labels was investigated. Twenty-four retarded adolescents were assigned to each of three conditions (blocking plus instructions, blocking plus labels, and blocking) for pretraining and training. Immediately after training, all subjects received a transfer task consisting of a new randomly organized list containing words from the same (training) categories for one-half of the subjects and from different categories for the other half. When exposure to category labels accompanied blocking, results indicated increased clustering and recall during the training phase and a trend toward increased clustering during the transfer phase.", "PMID": 961724}
{"id": "pubmed23n0032_4598", "title": "Modifying maternal teaching style: effects of task arrangement on the match-to-sample performance of retarded preschool-age-children.", "content": "Twenty-one mothers and their retarded preschool-age children were observed during six teaching sessions. Following the first three sessions, each mother-child dyad was assigned to one of the three groups. Groups were matched on measures of mother and child behavior and on measures of various mother and child background factors. Prior to the last three teaching sessions, mothers received instructions to modify certain aspects of their teaching style. The results indicated that children of mothers who had been instructed to present the materials of the task systematically obtained significantly higher performance scores during training than did children of mothers who either received no instruction or had been told to increase positive feedback for correct responses. Further, 6 of 7 children whose mothers had altered the manner in which they presented the task materials showed improvement on a test administered after training. These results suggest that nonverbal activities which precede responding are critical aspects of teaching style and deserve more attention than they have received in the past.", "contents": "Modifying maternal teaching style: effects of task arrangement on the match-to-sample performance of retarded preschool-age-children. Twenty-one mothers and their retarded preschool-age children were observed during six teaching sessions. Following the first three sessions, each mother-child dyad was assigned to one of the three groups. Groups were matched on measures of mother and child behavior and on measures of various mother and child background factors. Prior to the last three teaching sessions, mothers received instructions to modify certain aspects of their teaching style. The results indicated that children of mothers who had been instructed to present the materials of the task systematically obtained significantly higher performance scores during training than did children of mothers who either received no instruction or had been told to increase positive feedback for correct responses. Further, 6 of 7 children whose mothers had altered the manner in which they presented the task materials showed improvement on a test administered after training. These results suggest that nonverbal activities which precede responding are critical aspects of teaching style and deserve more attention than they have received in the past.", "PMID": 961725}
{"id": "pubmed23n0032_4599", "title": "Acquisition of concepts by TMR children as a function of type of modeling, rule verbalization, and observer gender.", "content": "A four-factor repeated measures design was established in order to determine (a) whether or not TMR children could acquire a novel rule-governed concept via modeling and (b) the effects of type of modeling demonstration, verbal rule provision, and observer gender on concept matching and transfer by TMR children. Twenty-four male and 24 female TMR children (mean IQ=44 and mean MA=5.67 years) were divided into four groups each equated by stratified assignment on IQ and MA. One male and one female group were randomly selected to observe a live female model present (a) massed-demonstration trials while verbalizing the concept, (b) massed trials without the concept being verbalized, (c) distributed-demonstration trials with the conceptual verbalization, and (d) distributed trials without conceptual verbalization. Subjects were then presented with three transfer tasks, each subsequently requiring a greater degree of generalization from the demonstration task. It was found that TMR children could acquire and transfer the complex concept via modeling. Distributed demonstrations yielded better initial response matching, while massed demonstrations led to better transfer. Rule provision facilitated transfer for massed-demonstration groups and facilitated matching for the male distributed-trials groups.", "contents": "Acquisition of concepts by TMR children as a function of type of modeling, rule verbalization, and observer gender. A four-factor repeated measures design was established in order to determine (a) whether or not TMR children could acquire a novel rule-governed concept via modeling and (b) the effects of type of modeling demonstration, verbal rule provision, and observer gender on concept matching and transfer by TMR children. Twenty-four male and 24 female TMR children (mean IQ=44 and mean MA=5.67 years) were divided into four groups each equated by stratified assignment on IQ and MA. One male and one female group were randomly selected to observe a live female model present (a) massed-demonstration trials while verbalizing the concept, (b) massed trials without the concept being verbalized, (c) distributed-demonstration trials with the conceptual verbalization, and (d) distributed trials without conceptual verbalization. Subjects were then presented with three transfer tasks, each subsequently requiring a greater degree of generalization from the demonstration task. It was found that TMR children could acquire and transfer the complex concept via modeling. Distributed demonstrations yielded better initial response matching, while massed demonstrations led to better transfer. Rule provision facilitated transfer for massed-demonstration groups and facilitated matching for the male distributed-trials groups.", "PMID": 961726}
{"id": "pubmed23n0032_4600", "title": "Assessment of the physical work capacity of institutionalized mentally retarded males.", "content": "Educable and trainable mentally retarded males (N=37) were examined for physical work capacity. Analysis of results indicated that the physical work capacity of the test population was 20 to 30% below that cited in the literature for nonretarded subjects of similar age and sex. Evidence also suggested that developmental and maintenance programs of physical fitness were required in order for mentally retarded persons to qualify for and maintain employment on most of the manual occupational tasks cited.", "contents": "Assessment of the physical work capacity of institutionalized mentally retarded males. Educable and trainable mentally retarded males (N=37) were examined for physical work capacity. Analysis of results indicated that the physical work capacity of the test population was 20 to 30% below that cited in the literature for nonretarded subjects of similar age and sex. Evidence also suggested that developmental and maintenance programs of physical fitness were required in order for mentally retarded persons to qualify for and maintain employment on most of the manual occupational tasks cited.", "PMID": 961727}
{"id": "pubmed23n0032_4601", "title": "Eye movements and conservation acceleration in mildly retarded children.", "content": "Thirty mildly retarded elementary-school children, 15 of whom had received perceptual/attentional training, were tested on four Piagetian conservation tasks (number, length, and continuous quantity solid/liquid) presented on 16 mm movie film with taped instructions. Eye movements were recorded during the response period for each task. Subsequent analyses of eye-movement patterns showed clear differences between the training and control groups. Trained conservers showed more visual exploratory activity and less perceptual centration than control group subjects. Moreover the eye-movement patterns of trained conservers closely approximated those shown by natural (i.e., untrained) retarded conservers in a previous study. The findings were discussed in terms of possible cognitive structural changes resulting from training and possible cognitive structural differences between mildly retarded and nonretarded children.", "contents": "Eye movements and conservation acceleration in mildly retarded children. Thirty mildly retarded elementary-school children, 15 of whom had received perceptual/attentional training, were tested on four Piagetian conservation tasks (number, length, and continuous quantity solid/liquid) presented on 16 mm movie film with taped instructions. Eye movements were recorded during the response period for each task. Subsequent analyses of eye-movement patterns showed clear differences between the training and control groups. Trained conservers showed more visual exploratory activity and less perceptual centration than control group subjects. Moreover the eye-movement patterns of trained conservers closely approximated those shown by natural (i.e., untrained) retarded conservers in a previous study. The findings were discussed in terms of possible cognitive structural changes resulting from training and possible cognitive structural differences between mildly retarded and nonretarded children.", "PMID": 961728}
{"id": "pubmed23n0032_4602", "title": "Nurses' expectations for accomplishment of mentally retarded patients.", "content": "As part of a larger study into the pattern of care delivered to hospitalized mentally retarded adults, an attitude survey was undertaken. Factor analysis on questionnaires returned by 696 nurses yielded a three factor solution. One factor, concerned with the expectations that nurses hold of the potential level of patient accomplishment, was examined. Different grades of staff were found to hold significantly different views on a scale ranging from \"optimist\" to \"pessimist\". Results from 30 hospitals showed that, after correcting for this grade effect, there were substantial institutional differences.", "contents": "Nurses' expectations for accomplishment of mentally retarded patients. As part of a larger study into the pattern of care delivered to hospitalized mentally retarded adults, an attitude survey was undertaken. Factor analysis on questionnaires returned by 696 nurses yielded a three factor solution. One factor, concerned with the expectations that nurses hold of the potential level of patient accomplishment, was examined. Different grades of staff were found to hold significantly different views on a scale ranging from \"optimist\" to \"pessimist\". Results from 30 hospitals showed that, after correcting for this grade effect, there were substantial institutional differences.", "PMID": 961729}
{"id": "pubmed23n0032_4603", "title": "Life expectancy of mentally retarded persons in Canadian institutions.", "content": "Data obtained from Statistics Canada were presented on the mortality level and expectancy of life for profoundly retarded and severely and moderately retarded persons in Canadian institutions for the years 1966 through 1968. Previous studies of mortality statistics were reported in mortality rates, average age at death, and crude death rates which are affected by the age distribution of the population involved. The very young and the very old are underrepresented in institutions, and thus these measures are not as accurate as life expectancy tables, which present the number of years expected to live, are independent of age distribution, and provide a reliable statistical measure for future replication and international comparisons. Retarded persons in institutions are living longer than previously, but their life expectancy does not meet that of the general population. Estimates of life expectancy for this population are vital for planning purposes.", "contents": "Life expectancy of mentally retarded persons in Canadian institutions. Data obtained from Statistics Canada were presented on the mortality level and expectancy of life for profoundly retarded and severely and moderately retarded persons in Canadian institutions for the years 1966 through 1968. Previous studies of mortality statistics were reported in mortality rates, average age at death, and crude death rates which are affected by the age distribution of the population involved. The very young and the very old are underrepresented in institutions, and thus these measures are not as accurate as life expectancy tables, which present the number of years expected to live, are independent of age distribution, and provide a reliable statistical measure for future replication and international comparisons. Retarded persons in institutions are living longer than previously, but their life expectancy does not meet that of the general population. Estimates of life expectancy for this population are vital for planning purposes.", "PMID": 961730}
{"id": "pubmed23n0032_4604", "title": "Utility of the Uzgiris and Hunt seales of sensorimotor development with severely and profoundly retarded children.", "content": "The reliability and validity of the Uzgiris and Hunt (1975) scales of sensorimotor development were examined with severely and profoundly retarded children. The subjects were 63 severely or profoundly retarded children between 42 and 126 months of age. Thirty of the subjects were living in residential facilities, and 33 were living at home and attending day schools. All of the subjects were assessed on all six scales of the Uzgiris and Hunt instrument. Interexaminer and test-retest reliabilities were computed. Scalogram analyses were also computed for all but the Schemes scale. The findings were that the scales were reliable and, as theorized by Piaget, ordinal with the present sample. These findings were interpreted as indicating that these scales could be used reliably and validly with severely and profoundly retarded children. This was discussed in terms of the present and potential uses of these scales.", "contents": "Utility of the Uzgiris and Hunt seales of sensorimotor development with severely and profoundly retarded children. The reliability and validity of the Uzgiris and Hunt (1975) scales of sensorimotor development were examined with severely and profoundly retarded children. The subjects were 63 severely or profoundly retarded children between 42 and 126 months of age. Thirty of the subjects were living in residential facilities, and 33 were living at home and attending day schools. All of the subjects were assessed on all six scales of the Uzgiris and Hunt instrument. Interexaminer and test-retest reliabilities were computed. Scalogram analyses were also computed for all but the Schemes scale. The findings were that the scales were reliable and, as theorized by Piaget, ordinal with the present sample. These findings were interpreted as indicating that these scales could be used reliably and validly with severely and profoundly retarded children. This was discussed in terms of the present and potential uses of these scales.", "PMID": 961731}
{"id": "pubmed23n0032_4605", "title": "Sensory reinforcement of eyeblink rate in a decorticate human.", "content": "An unusual case of hydranencephaly was reported. The child survived for 19 years and showed evidence on three occasions of an increase in eyeblink rate with tactile reinforcement. Diagnosis was confirmed by an autopsy which revealed no preserved cortex in either hemisphere.", "contents": "Sensory reinforcement of eyeblink rate in a decorticate human. An unusual case of hydranencephaly was reported. The child survived for 19 years and showed evidence on three occasions of an increase in eyeblink rate with tactile reinforcement. Diagnosis was confirmed by an autopsy which revealed no preserved cortex in either hemisphere.", "PMID": 961732}
{"id": "pubmed23n0032_4606", "title": "Role-taking ability and the interpersonal tactics of retarded children.", "content": "The relationship between social role-taking ability and preferences for interpersonal control tactics was assessed in 50 mentally retarded children. Feffer's Role-Taking Task and two hypothetical interpersonal control episodes were used as experimental measures. Results were that role-taking proficiency was associated with the development of alter-directed tactics or tactics by which the subject appeared to take into account the needs, motivations, or expectations of others. Neither MA nor IQ were significant predictors of interpersonal tactic choice. Controls for IQ and MA did not alter the significance of the association between role-taking and general tactic preferences.", "contents": "Role-taking ability and the interpersonal tactics of retarded children. The relationship between social role-taking ability and preferences for interpersonal control tactics was assessed in 50 mentally retarded children. Feffer's Role-Taking Task and two hypothetical interpersonal control episodes were used as experimental measures. Results were that role-taking proficiency was associated with the development of alter-directed tactics or tactics by which the subject appeared to take into account the needs, motivations, or expectations of others. Neither MA nor IQ were significant predictors of interpersonal tactic choice. Controls for IQ and MA did not alter the significance of the association between role-taking and general tactic preferences.", "PMID": 961733}
{"id": "pubmed23n0032_4607", "title": "A new approach to patients with suspected anaerobic postpartum pelvic infections. Transabdominal uterine aspiration for culture and metronidazole for treatment.", "content": "Oral metronidazole was given as therapy in 25 women with the clinical diagnosis of a postpartum anaerobic soft tissue pelvic infection following a vaginal delivery. There was a good clinical response in 80 per cent of these women. No anaerobic organisms resistant to metronidazole were recovered in the treatment failures. Different specimen collection techniques were evaluated, with anaerobes recovered in 4 per cent of the blood cultures, 26.3 per cent of the transabdominal endometrial aspirations, and 88 per cent of the transcervical endometrial samples. The aerobic recovery of organisms was 4 per cent from blood cultures, 15.8 per cent from transabdominal endometrial aspirations, and 96 per cent from the transcervical approach. The significance of these findings is discussed.", "contents": "A new approach to patients with suspected anaerobic postpartum pelvic infections. Transabdominal uterine aspiration for culture and metronidazole for treatment. Oral metronidazole was given as therapy in 25 women with the clinical diagnosis of a postpartum anaerobic soft tissue pelvic infection following a vaginal delivery. There was a good clinical response in 80 per cent of these women. No anaerobic organisms resistant to metronidazole were recovered in the treatment failures. Different specimen collection techniques were evaluated, with anaerobes recovered in 4 per cent of the blood cultures, 26.3 per cent of the transabdominal endometrial aspirations, and 88 per cent of the transcervical endometrial samples. The aerobic recovery of organisms was 4 per cent from blood cultures, 15.8 per cent from transabdominal endometrial aspirations, and 96 per cent from the transcervical approach. The significance of these findings is discussed.", "PMID": 961734}
{"id": "pubmed23n0032_4608", "title": "Carcinoembryonic antigen in intraepithelial neoplasia of the uterine cervix.", "content": "Carcinoembryonic antigen (CEA) was elevated (greater than 2.5 ng. per milliliter) in 29 of 100 patients with cervical intraepithelial neoplasia (CIN). CEA concentration was related to the amount of intraepithelial neoplasia and to the presence of glandular extension. Lymphoplasmacytic infiltration of tumor cells was unrelated to CEA levels. CEA values returned to normal within 8 weeks following surgery in 77 per cent of patients. A persistently elevated (greater than 5.0 ng. milliter) plasma CEA value following conization was associated with residual CIN in the cervix. These results suggest that sequential CEA determinations may be of value in the follow-up of those cervical cancer patients who initially have high plasma antigen levels.", "contents": "Carcinoembryonic antigen in intraepithelial neoplasia of the uterine cervix. Carcinoembryonic antigen (CEA) was elevated (greater than 2.5 ng. per milliliter) in 29 of 100 patients with cervical intraepithelial neoplasia (CIN). CEA concentration was related to the amount of intraepithelial neoplasia and to the presence of glandular extension. Lymphoplasmacytic infiltration of tumor cells was unrelated to CEA levels. CEA values returned to normal within 8 weeks following surgery in 77 per cent of patients. A persistently elevated (greater than 5.0 ng. milliter) plasma CEA value following conization was associated with residual CIN in the cervix. These results suggest that sequential CEA determinations may be of value in the follow-up of those cervical cancer patients who initially have high plasma antigen levels.", "PMID": 961735}
{"id": "pubmed23n0032_4609", "title": "A hypothesis: the role of a high-risk male in the etiology of cervical carcinoma: a correlation of epidemiology and molecular biology.", "content": "Certain impressive epidemiological evidence appearing in the last few years leads to the conclusion that some males are more prone to have a spouse with squamous cancer of the cervix than others. Advances in knowledge in other fields, especially at the molecular biological level, enable speculation on reasons for this curious conclusion. These readily testable theories, together with an outline of the evidence on which they rest, form the substance of this hypothesis.", "contents": "A hypothesis: the role of a high-risk male in the etiology of cervical carcinoma: a correlation of epidemiology and molecular biology. Certain impressive epidemiological evidence appearing in the last few years leads to the conclusion that some males are more prone to have a spouse with squamous cancer of the cervix than others. Advances in knowledge in other fields, especially at the molecular biological level, enable speculation on reasons for this curious conclusion. These readily testable theories, together with an outline of the evidence on which they rest, form the substance of this hypothesis.", "PMID": 961736}
{"id": "pubmed23n0032_4610", "title": "Outpatient evaluation of patients with atypical Papanicolaou smears: contribution of endocervical curettage.", "content": "A series of 603 patients referred with atypical Papanicolaou smears was evaluated by repeat smears, colposcopically directed cervical biopsies, and endocervical curettage. These techniques as a unit can establish an accurate outpatient diagnosis superior to any of these modalities used alone and comparable with findings in conization and hysterectomy specimens. Endocervical curettage has made a unique contribution to the evaluation of such patients; these curettings have allowed examination of tissue fragments and are more reliable in diagnosing neoplasia than are endocervical smears. Invasive carcinoma and its precursors confined to the anatomic endocervical canal can be recognized by this technique, and conversely the absence of neoplastic epithelium in adequate endocervical curettings rules out occult carcinoma. Indications for conization of the cervix are discussed in reference to the other biopsy and cytologic findings, and guidelines are presented for patient management, stressing clinicopathologic correlation and cooperation.", "contents": "Outpatient evaluation of patients with atypical Papanicolaou smears: contribution of endocervical curettage. A series of 603 patients referred with atypical Papanicolaou smears was evaluated by repeat smears, colposcopically directed cervical biopsies, and endocervical curettage. These techniques as a unit can establish an accurate outpatient diagnosis superior to any of these modalities used alone and comparable with findings in conization and hysterectomy specimens. Endocervical curettage has made a unique contribution to the evaluation of such patients; these curettings have allowed examination of tissue fragments and are more reliable in diagnosing neoplasia than are endocervical smears. Invasive carcinoma and its precursors confined to the anatomic endocervical canal can be recognized by this technique, and conversely the absence of neoplastic epithelium in adequate endocervical curettings rules out occult carcinoma. Indications for conization of the cervix are discussed in reference to the other biopsy and cytologic findings, and guidelines are presented for patient management, stressing clinicopathologic correlation and cooperation.", "PMID": 961737}
{"id": "pubmed23n0032_4611", "title": "Volatile constituents of human vaginal secretions.", "content": "Vaginal secretions were serially studied for 44 ovulatory cycles from 12 patients by means of combined gas chromatograph--mass spectrometry to identify small organic volatile compounds. In 10 of these cycles ovulation was documented with plasma radoioimmunoassays for progesterone, estrogens, and LH. These secretions contain a complex mixture of acids, alcohols, hydroxyketones, and aromatic compounds. Lactic acid, acetic acid, and urea were found to be present in all patients and underwent sharp cyclical variations in concentration with maxima in all patients occurring at midcycle. Small-chain, volatile C2-C5 aliphatic acids which have been shown to induce mating behavior in male rhesus monkeys were found in only four patients. In these patients the above acids were found predominantly at midcycle and during the luteal phase.", "contents": "Volatile constituents of human vaginal secretions. Vaginal secretions were serially studied for 44 ovulatory cycles from 12 patients by means of combined gas chromatograph--mass spectrometry to identify small organic volatile compounds. In 10 of these cycles ovulation was documented with plasma radoioimmunoassays for progesterone, estrogens, and LH. These secretions contain a complex mixture of acids, alcohols, hydroxyketones, and aromatic compounds. Lactic acid, acetic acid, and urea were found to be present in all patients and underwent sharp cyclical variations in concentration with maxima in all patients occurring at midcycle. Small-chain, volatile C2-C5 aliphatic acids which have been shown to induce mating behavior in male rhesus monkeys were found in only four patients. In these patients the above acids were found predominantly at midcycle and during the luteal phase.", "PMID": 961738}
{"id": "pubmed23n0032_4612", "title": "Primary prostaglandins in amniotic fluid in pregnancy and spontaneous labor.", "content": "Primary prostaglandins have been measured by radioimmunoassay in the amniotic fluid of 80 patients. In late pregnancy, PGE2 was higher than PGF2alpha with an increase around 36 weeks (378 +/- 71 pg. per milliliter) without significant modification until term. PGF2alpha increased too, but only significantly at term. During spontaneous labor, PGE2 and PGF2alpha were higher than before the onset of labor. Through serial determinations in five patients, two steps can be distinguished: the first one with stable levels but large oscillations of PGE2 and PGF2alpha and the second one starting at a variable stage of cervical dilatation with inversion of PGE2/PGF2alpha ratio (less than 1 and followed by an increase of both compounds more pronounced for PGF2alpha. It appears that PGE2 is concerned with the initiation of parturition while both PG's and mainly PGF2alpha are in relation to the contractions and the duration of labor.", "contents": "Primary prostaglandins in amniotic fluid in pregnancy and spontaneous labor. Primary prostaglandins have been measured by radioimmunoassay in the amniotic fluid of 80 patients. In late pregnancy, PGE2 was higher than PGF2alpha with an increase around 36 weeks (378 +/- 71 pg. per milliliter) without significant modification until term. PGF2alpha increased too, but only significantly at term. During spontaneous labor, PGE2 and PGF2alpha were higher than before the onset of labor. Through serial determinations in five patients, two steps can be distinguished: the first one with stable levels but large oscillations of PGE2 and PGF2alpha and the second one starting at a variable stage of cervical dilatation with inversion of PGE2/PGF2alpha ratio (less than 1 and followed by an increase of both compounds more pronounced for PGF2alpha. It appears that PGE2 is concerned with the initiation of parturition while both PG's and mainly PGF2alpha are in relation to the contractions and the duration of labor.", "PMID": 961739}
{"id": "pubmed23n0032_4613", "title": "Placental prostaglandin levels in pre-eclampsia.", "content": "Pre-eclampsia is characterized by uteroplacental ischemia. Prostaglandins can alter systemic blood pressure as well as regulate blood flow to the fetoplacental unit. In the present study, levels of prostaglandin E were significantly decreased in placental tissue from pre-eclamptic patients. Prostaglandin F, a potent vasoconstrictor, was markedly elevated. These observations indicate that altered placental metabolism of prostaglandins is an important factor in the pathophysiology of pre-eclampsia.", "contents": "Placental prostaglandin levels in pre-eclampsia. Pre-eclampsia is characterized by uteroplacental ischemia. Prostaglandins can alter systemic blood pressure as well as regulate blood flow to the fetoplacental unit. In the present study, levels of prostaglandin E were significantly decreased in placental tissue from pre-eclamptic patients. Prostaglandin F, a potent vasoconstrictor, was markedly elevated. These observations indicate that altered placental metabolism of prostaglandins is an important factor in the pathophysiology of pre-eclampsia.", "PMID": 961740}
{"id": "pubmed23n0032_4614", "title": "Oral contraceptives--induced hypertension--nine years later.", "content": "Nine years have gone by since oral contraceptive hypertension was first recognized as a clinical entity. In that time it has become apparent that what at first was thought to be a rare disorder is extremely common. Indeed, overt hypertension develops, in time, in about 5 per cent of Pill users. Increases in blood pressure, albeit within the normal range, are still more common. Pill hypertension may develop gradually. It is sometimes quite severe, and it is characteristically reversible within a few months after therapy is stopped. Oral contraceptives produce changes in the renin-angiotensin-aldosterone system, particularly a consistent marked increase in the plasma renin substrate concentration which may be associated with increases in plasma renin activity and aldosterone excretion. The estrogenic component of contraceptive pills appears to be the more important factor in producing abnormalities in the renin system. The mechanisms for the hypertensive response are not entirely clear since normotensive women using the pill may exhibit similar or even more marked changes in the renin axis. The pressor response could have both volume and vasoconstrictor components mediated by the sodium-retaining effects of the estrogen in the presence of a relatively, if not an absolutely, higher plasma renin activity, with the latter being held abnormal by the high plasma renin-substrate levels. A failure of the kidneys to fully suppress renal renin secretion could thus be an important predisposing factor. Our in vitro experiments support the idea that the increased substrate is involved in pathogenesis because they indicate that in normal subjects plasma renin substrate is not present in \"excess\". Thus, a doubling of the physiologic levels of substrate by oral contraceptives leads to an almost twofold increase in the capacity for angiotensin production as indicated by studies of the initial reaction velocity. These observations define certain guidelines for applying oral contraceptive therapy. At least one base-line blood pressure measurement should be obtained, and blood pressure and weight should be followed a two- or three-month intervals during treatment. Furthermore, oral contraceptive therapy may be contraindicated in women with a history of hypertension, renal disease, toxemia, or fluid retention. Others in whom oral contraceptives are relatively contraindicated include those with a positive family history of hypertension, younger women in whom a longer term commitment is likely, and groups, such as blacks, especially prone to hypertensive phenomena.", "contents": "Oral contraceptives--induced hypertension--nine years later. Nine years have gone by since oral contraceptive hypertension was first recognized as a clinical entity. In that time it has become apparent that what at first was thought to be a rare disorder is extremely common. Indeed, overt hypertension develops, in time, in about 5 per cent of Pill users. Increases in blood pressure, albeit within the normal range, are still more common. Pill hypertension may develop gradually. It is sometimes quite severe, and it is characteristically reversible within a few months after therapy is stopped. Oral contraceptives produce changes in the renin-angiotensin-aldosterone system, particularly a consistent marked increase in the plasma renin substrate concentration which may be associated with increases in plasma renin activity and aldosterone excretion. The estrogenic component of contraceptive pills appears to be the more important factor in producing abnormalities in the renin system. The mechanisms for the hypertensive response are not entirely clear since normotensive women using the pill may exhibit similar or even more marked changes in the renin axis. The pressor response could have both volume and vasoconstrictor components mediated by the sodium-retaining effects of the estrogen in the presence of a relatively, if not an absolutely, higher plasma renin activity, with the latter being held abnormal by the high plasma renin-substrate levels. A failure of the kidneys to fully suppress renal renin secretion could thus be an important predisposing factor. Our in vitro experiments support the idea that the increased substrate is involved in pathogenesis because they indicate that in normal subjects plasma renin substrate is not present in \"excess\". Thus, a doubling of the physiologic levels of substrate by oral contraceptives leads to an almost twofold increase in the capacity for angiotensin production as indicated by studies of the initial reaction velocity. These observations define certain guidelines for applying oral contraceptive therapy. At least one base-line blood pressure measurement should be obtained, and blood pressure and weight should be followed a two- or three-month intervals during treatment. Furthermore, oral contraceptive therapy may be contraindicated in women with a history of hypertension, renal disease, toxemia, or fluid retention. Others in whom oral contraceptives are relatively contraindicated include those with a positive family history of hypertension, younger women in whom a longer term commitment is likely, and groups, such as blacks, especially prone to hypertensive phenomena.", "PMID": 961741}
{"id": "pubmed23n0032_4615", "title": "Systolic time intervals in normotensive and hypertensive human pregnancy.", "content": "Systolic time intervals were measured in 10 nonpregnant, 37 normotensive, and 18 hypertensive pregnant women in both supine and lateral positions. With all the subjects in the supine position, left ventricular ejection time (LVET) was shortened; the pre-ejection period (PEP) lengthened, and the PEP/LVET ratio increased in normotensive late pregnancy compared with the nonpregnant state. Similar alterations in systolic time intervals were observed in hypertensive women in both early and late pregnancy in the supine position. In normotensive women in early pregnancy, alterations in systolic time intervals were inconclusive. When normotensive women in late pregnancy were turned from the supine into the left lateral position, a prolongation of LVET and a decrease in the PEP/LVET ratio were observed. When hypertensive women in late pregnancy adopted the left lateral position, no significant alterations in systolic time intervals occurred. Isovolumetric contraction time (ICT) was prolonged only in the hypertensive pregnant women in the supine position. The study suggests that left ventricular performance is diminished in normotensive women in late pregnancy when supine but improves when they adopt the lateral position. In addition, hypertensive pregnant women show evidence of diminished left ventricular performance which is not improved in late pregnancy by assumption of the left lateral position.", "contents": "Systolic time intervals in normotensive and hypertensive human pregnancy. Systolic time intervals were measured in 10 nonpregnant, 37 normotensive, and 18 hypertensive pregnant women in both supine and lateral positions. With all the subjects in the supine position, left ventricular ejection time (LVET) was shortened; the pre-ejection period (PEP) lengthened, and the PEP/LVET ratio increased in normotensive late pregnancy compared with the nonpregnant state. Similar alterations in systolic time intervals were observed in hypertensive women in both early and late pregnancy in the supine position. In normotensive women in early pregnancy, alterations in systolic time intervals were inconclusive. When normotensive women in late pregnancy were turned from the supine into the left lateral position, a prolongation of LVET and a decrease in the PEP/LVET ratio were observed. When hypertensive women in late pregnancy adopted the left lateral position, no significant alterations in systolic time intervals occurred. Isovolumetric contraction time (ICT) was prolonged only in the hypertensive pregnant women in the supine position. The study suggests that left ventricular performance is diminished in normotensive women in late pregnancy when supine but improves when they adopt the lateral position. In addition, hypertensive pregnant women show evidence of diminished left ventricular performance which is not improved in late pregnancy by assumption of the left lateral position.", "PMID": 961742}
{"id": "pubmed23n0032_4616", "title": "The relationship of hospital-acquired infection to invasive intrapartum monitoring techniques.", "content": "A 6 month survey of hospital-acquired uterine infection was done on the obstetric service of the Los Angeles County-University of Southern California (LAS-USC) Medical Center to determine the impact of the duration of monitoring upon the incidence of maternal infection. There were significantly more infections among the population monitored and in women undergoing cesarean section. In diminishing order, the incidence of infection was: cesarean section monitored, 90 of 223 (40.4 per cent); cesarean section not monitored, 56 of 271 (20.4 per cent); elective repeat cesarean section, 1 of 21 (5 per cent); vaginal delivery monitored, 33 of 1,236 (2.7 per cent); and vaginal delivery not monitored, 49 of 3,445 (1.4 per cent). In comparing those patients with infection and those free of infection following equivalent routes of delivery, there was no statistical difference in the duration of monitoring. These results suggest that the interval of monitoring alone is not a significant clinical factor in the development of maternal soft-tissue pelvic infection in a high-risk obstetric population.", "contents": "The relationship of hospital-acquired infection to invasive intrapartum monitoring techniques. A 6 month survey of hospital-acquired uterine infection was done on the obstetric service of the Los Angeles County-University of Southern California (LAS-USC) Medical Center to determine the impact of the duration of monitoring upon the incidence of maternal infection. There were significantly more infections among the population monitored and in women undergoing cesarean section. In diminishing order, the incidence of infection was: cesarean section monitored, 90 of 223 (40.4 per cent); cesarean section not monitored, 56 of 271 (20.4 per cent); elective repeat cesarean section, 1 of 21 (5 per cent); vaginal delivery monitored, 33 of 1,236 (2.7 per cent); and vaginal delivery not monitored, 49 of 3,445 (1.4 per cent). In comparing those patients with infection and those free of infection following equivalent routes of delivery, there was no statistical difference in the duration of monitoring. These results suggest that the interval of monitoring alone is not a significant clinical factor in the development of maternal soft-tissue pelvic infection in a high-risk obstetric population.", "PMID": 961743}
{"id": "pubmed23n0032_4617", "title": "Renal clearances of estriol conjugates in normal human pregnancy at term.", "content": "In late human pregnancy more than 90 per cent of the total estriol (E3) in body fluids consists of four conjugates, estriol-3-sulfate (E3-3S), estriol-16-glucosiduronate (E3-16G), estriol-3-glucuronide (E3-3G), and estriol-3-sulfate-16-glucosiduronate (E3-3S-16G). Since the relative amounts of E3 in blood and urine would be determined by the kidney, the renal clearance of each conjugate was determined and compared with inulin and p-aminohippuric acid (PAH) clearance, as measures of glomerular filtration rate (GFR) and the effective renal plasma flow. Five women were studied in the lateral decubitus position with inulin and PAH infusion. Samples of blood and urine were collected at 40 minute intervals and analyzed. The method for E3 conjugates involved separation of the four conjugates on Sephadex LH-20, enzyme hydrolysis, and radioimmunoassay. Renal clearances for E3-3S and E3-3S-16G were below inulin. E3-3G approximated inulin; E3-16G exceeded inulin and approached PAH. In plasma E3-3S-16G represented 48.4 +/- 7.2 per cent; in urine E3-16G represented 69.5 +/- 7.3 per cent of total E3. Thus, different conjugates predominate in blood and urine.", "contents": "Renal clearances of estriol conjugates in normal human pregnancy at term. In late human pregnancy more than 90 per cent of the total estriol (E3) in body fluids consists of four conjugates, estriol-3-sulfate (E3-3S), estriol-16-glucosiduronate (E3-16G), estriol-3-glucuronide (E3-3G), and estriol-3-sulfate-16-glucosiduronate (E3-3S-16G). Since the relative amounts of E3 in blood and urine would be determined by the kidney, the renal clearance of each conjugate was determined and compared with inulin and p-aminohippuric acid (PAH) clearance, as measures of glomerular filtration rate (GFR) and the effective renal plasma flow. Five women were studied in the lateral decubitus position with inulin and PAH infusion. Samples of blood and urine were collected at 40 minute intervals and analyzed. The method for E3 conjugates involved separation of the four conjugates on Sephadex LH-20, enzyme hydrolysis, and radioimmunoassay. Renal clearances for E3-3S and E3-3S-16G were below inulin. E3-3G approximated inulin; E3-16G exceeded inulin and approached PAH. In plasma E3-3S-16G represented 48.4 +/- 7.2 per cent; in urine E3-16G represented 69.5 +/- 7.3 per cent of total E3. Thus, different conjugates predominate in blood and urine.", "PMID": 961744}
{"id": "pubmed23n0032_4618", "title": "Neonatal respiratory distress following elective delivery. A preventable disease?", "content": "Twelve per cent of all infants with respiratory distress admitted to our neonatal intensive-care unit from November, 1973 to April, 1974, were born after elective intervention (15 cesarean sections and four vaginal inductions). All were white and 18/19 were private compared to yearly admissions of white (56 per cent) and private (57 per cent). Eighteen of 19 were admitted from the region via the transport service. Mean birth weight was 2.69 kilograms, with 18 infants over 2 kilograms. Pediatric gestational age from a physical and neurological evaluation ranged from 32 to 39 weeks (mean 36.2 weeks) in contrast to obstetric dating which ranged from 38 to 44 weeks (mean 39 weeks). The obstetric dating was 3 or more weeks greater than the pediatric age in 11 infants. Pulmonary disease included transient tachypnea (5) and respiratory distress syndrome (14). No prior documentation of pulmonary maturity had been obtained in any of these infants. Mean hospitalization was 23 days (range 1 to 140), with estimated costs of $3,421 per baby. Two infants died. Respiratory distress following elective delivery remains a potent source of on-going perinatal morbidity. Regional programs must direct increased educational efforts to eliminate this preventable disease.", "contents": "Neonatal respiratory distress following elective delivery. A preventable disease? Twelve per cent of all infants with respiratory distress admitted to our neonatal intensive-care unit from November, 1973 to April, 1974, were born after elective intervention (15 cesarean sections and four vaginal inductions). All were white and 18/19 were private compared to yearly admissions of white (56 per cent) and private (57 per cent). Eighteen of 19 were admitted from the region via the transport service. Mean birth weight was 2.69 kilograms, with 18 infants over 2 kilograms. Pediatric gestational age from a physical and neurological evaluation ranged from 32 to 39 weeks (mean 36.2 weeks) in contrast to obstetric dating which ranged from 38 to 44 weeks (mean 39 weeks). The obstetric dating was 3 or more weeks greater than the pediatric age in 11 infants. Pulmonary disease included transient tachypnea (5) and respiratory distress syndrome (14). No prior documentation of pulmonary maturity had been obtained in any of these infants. Mean hospitalization was 23 days (range 1 to 140), with estimated costs of $3,421 per baby. Two infants died. Respiratory distress following elective delivery remains a potent source of on-going perinatal morbidity. Regional programs must direct increased educational efforts to eliminate this preventable disease.", "PMID": 961745}
{"id": "pubmed23n0032_4619", "title": "The response of the human Fallopian tube to ergonovine and methyl-ergonovine in vivo.", "content": "Ergonovine maleate (EM) is a powerful stimulant of human tubal motility. It has been therefore thought that EM could interfere with normal ovum transport and serve as a contraceptive. The tube activating effects of EM and methyl EM were evaluated in 14 women and results confirmed that both are powerful stimulants of the human Fallopian tube. EM immediately post coitus significantly reduces the conception rate.", "contents": "The response of the human Fallopian tube to ergonovine and methyl-ergonovine in vivo. Ergonovine maleate (EM) is a powerful stimulant of human tubal motility. It has been therefore thought that EM could interfere with normal ovum transport and serve as a contraceptive. The tube activating effects of EM and methyl EM were evaluated in 14 women and results confirmed that both are powerful stimulants of the human Fallopian tube. EM immediately post coitus significantly reduces the conception rate.", "PMID": 961746}
{"id": "pubmed23n0032_4620", "title": "Suboptimal pregnancy outcome among women with prior abortions and premature births.", "content": "Data from 25,958 consecutive UCLA deliveries were analyzed to determine the effect of prior abortions and premature births on current pregnancy outcome. Perinatal death rate, combining stillbirths and neonatal deaths, increased more than threefold among women with at least one prior premature in birth and at least one prior abortion and approached 18 per cent of current deliveries when there were three or more prior premature births. Abnormal live births, defined as infants with either birth weight under 2,501 grams, gestational age less than 37 weeks, or congenital anomalies, significantly increased as the number of prior abortions and premature births increased, each in a range of 0 through 3 or more. For example, among women with at least three prior premature births, there were greater than 50 per cent abnormal live births. The risk was mostly that of low birth weight and low gestational age, although there was a slight increase in congenital anomalies. The risk was reduced considerably when there were previous term births and was influenced variably by race, clinic classification, maternal illness, and prior pregnancy complications. This empirical data on pregnancy outcome should be useful in reproductive counseling among women with pregnancy losses and premature births.", "contents": "Suboptimal pregnancy outcome among women with prior abortions and premature births. Data from 25,958 consecutive UCLA deliveries were analyzed to determine the effect of prior abortions and premature births on current pregnancy outcome. Perinatal death rate, combining stillbirths and neonatal deaths, increased more than threefold among women with at least one prior premature in birth and at least one prior abortion and approached 18 per cent of current deliveries when there were three or more prior premature births. Abnormal live births, defined as infants with either birth weight under 2,501 grams, gestational age less than 37 weeks, or congenital anomalies, significantly increased as the number of prior abortions and premature births increased, each in a range of 0 through 3 or more. For example, among women with at least three prior premature births, there were greater than 50 per cent abnormal live births. The risk was mostly that of low birth weight and low gestational age, although there was a slight increase in congenital anomalies. The risk was reduced considerably when there were previous term births and was influenced variably by race, clinic classification, maternal illness, and prior pregnancy complications. This empirical data on pregnancy outcome should be useful in reproductive counseling among women with pregnancy losses and premature births.", "PMID": 961747}
{"id": "pubmed23n0032_4621", "title": "Antepartum fetal heart rate and uterine activity studies: I. Preliminary report of accelerations and the oxytocin challenge test.", "content": "A series of 344 antepartum fetal heart rate studies in 209 high-risk patients is described. The importance of evaluation of accelerations of the fetal heart rate as well as periodic decelerations associated with uterine activity is demonstrated. Our data suggest that the absence of accelerations of the fetal heart rate during the recording period may be associated with increased perinatal morbidity. Late decelerations may occur in the same recording session as accelerations of the fetal heart rate. The association of late decelerations of the fetal heart rate with no accelerations during the recording session is highly suggestive of increased perinatal morbidity. In high-risk patients, accelerations of the fetal heart rate and no late decelerations with uterine activity are a reassuring finding, with 91 per cent of patients showing no increased perinatal morbidity.", "contents": "Antepartum fetal heart rate and uterine activity studies: I. Preliminary report of accelerations and the oxytocin challenge test. A series of 344 antepartum fetal heart rate studies in 209 high-risk patients is described. The importance of evaluation of accelerations of the fetal heart rate as well as periodic decelerations associated with uterine activity is demonstrated. Our data suggest that the absence of accelerations of the fetal heart rate during the recording period may be associated with increased perinatal morbidity. Late decelerations may occur in the same recording session as accelerations of the fetal heart rate. The association of late decelerations of the fetal heart rate with no accelerations during the recording session is highly suggestive of increased perinatal morbidity. In high-risk patients, accelerations of the fetal heart rate and no late decelerations with uterine activity are a reassuring finding, with 91 per cent of patients showing no increased perinatal morbidity.", "PMID": 961748}
{"id": "pubmed23n0032_4622", "title": "Colonic pseudo-obstruction following termination of pregnancy and uterine operation.", "content": "Colonic pseudo-obstruction refers to a condition in which physical and radiologic findings identical to those associated with mechanical obstruction of the large bowel are found but in which no organic cause of the colonic distention is present. Cases of this condition have been reported in the world literature, of which 40 per cent have followed the termination of pregnancy or uterine operation. Three cases of this syndrome are presented. One followed normal vaginal delivery; one followed cesarean section, and the third followed elective abdominal hysterectomy. The literature on the subject is reviewed, and a possible anatomicophysiologic explanation, based upon a sympathetic-parasympathetic neurostimulatory imbalance, is put forward.", "contents": "Colonic pseudo-obstruction following termination of pregnancy and uterine operation. Colonic pseudo-obstruction refers to a condition in which physical and radiologic findings identical to those associated with mechanical obstruction of the large bowel are found but in which no organic cause of the colonic distention is present. Cases of this condition have been reported in the world literature, of which 40 per cent have followed the termination of pregnancy or uterine operation. Three cases of this syndrome are presented. One followed normal vaginal delivery; one followed cesarean section, and the third followed elective abdominal hysterectomy. The literature on the subject is reviewed, and a possible anatomicophysiologic explanation, based upon a sympathetic-parasympathetic neurostimulatory imbalance, is put forward.", "PMID": 961749}
{"id": "pubmed23n0032_4623", "title": "Classification of human fetal movement.", "content": "A study of fetal activity was undertaken as the first step in a series of physiologic fetal movement studies. A simple, safe, noninvasive, clinically applicable method for studying fetal movement with a tocodynamometer is described. Four basic types of fetal movement have been seen and defined in terms of their durations, recorded patterns, and descriptive terminologies for identifying each movement.", "contents": "Classification of human fetal movement. A study of fetal activity was undertaken as the first step in a series of physiologic fetal movement studies. A simple, safe, noninvasive, clinically applicable method for studying fetal movement with a tocodynamometer is described. Four basic types of fetal movement have been seen and defined in terms of their durations, recorded patterns, and descriptive terminologies for identifying each movement.", "PMID": 961750}
{"id": "pubmed23n0032_4624", "title": "Reported live births following induced abortion: two and one-half years' experience in Upstate New York.", "content": "Thirty-eight live births following induced abortion were recorded in Upstate New York between July, 1970, and December, 1972. Twenty-six followed saline-induced abortion; twelve of these occurred at one hospital, and eight were associated with one physician. Underestimation of gestation and exchange of inadequate volumes of amniotic fluid and hypertonic saline produced concentrations insufficient to cause intrauterine death. Viable infants with iatrogenic central nervous system damage from salt poisoning are a possible consequence of improperly planned saline-induced abortions. These births provide further evidence for encouragement of contraception and early termination of unwanted pregnancy.", "contents": "Reported live births following induced abortion: two and one-half years' experience in Upstate New York. Thirty-eight live births following induced abortion were recorded in Upstate New York between July, 1970, and December, 1972. Twenty-six followed saline-induced abortion; twelve of these occurred at one hospital, and eight were associated with one physician. Underestimation of gestation and exchange of inadequate volumes of amniotic fluid and hypertonic saline produced concentrations insufficient to cause intrauterine death. Viable infants with iatrogenic central nervous system damage from salt poisoning are a possible consequence of improperly planned saline-induced abortions. These births provide further evidence for encouragement of contraception and early termination of unwanted pregnancy.", "PMID": 961751}
{"id": "pubmed23n0032_4625", "title": "Ovarian morphology in women with anosmia and hypogonadotropic hypogonadism.", "content": "Ovarian biopsies from five women with the syndrome of hypogonadotropic hypogonadism and anosmia who had not previously received exogenous gonadotropins were evaluated with special emphasis on follicular morphology. Follicular development past the primordial stage was rarely observed, suggesting that early stages of follicular maturation require amounts of gonadotropins in excess of those secreted in these women.", "contents": "Ovarian morphology in women with anosmia and hypogonadotropic hypogonadism. Ovarian biopsies from five women with the syndrome of hypogonadotropic hypogonadism and anosmia who had not previously received exogenous gonadotropins were evaluated with special emphasis on follicular morphology. Follicular development past the primordial stage was rarely observed, suggesting that early stages of follicular maturation require amounts of gonadotropins in excess of those secreted in these women.", "PMID": 961752}
{"id": "pubmed23n0032_4626", "title": "Excision of submucus fibroids with hysteroscopic control.", "content": "Hysteroscopy offers a new option in the diagnosis and management of submucus fibroids. The hysteroscopic excision of submucus fibroids in five cases is described. The problems and potentials of this approach are discussed.", "contents": "Excision of submucus fibroids with hysteroscopic control. Hysteroscopy offers a new option in the diagnosis and management of submucus fibroids. The hysteroscopic excision of submucus fibroids in five cases is described. The problems and potentials of this approach are discussed.", "PMID": 961753}
{"id": "pubmed23n0032_4627", "title": "Rational management of perinatal hydrocephalus.", "content": "Hydrocephalus of prenatal origin, which may be hereditary, is likely to be discovered more frequently and earlier in gestation with ultrasonic diagnosis. It is suggested that each case be analyzed according to a protocol which includes a full obstetric evaluation, complete genetic pedigree, determination of familial cranial size, and complete neuropathologic study. Destruction of the aqueductal area may deprive the investigator of much of the information needed for the thorough study of the case which is vital to counsel the parents concerning future pregnancies.", "contents": "Rational management of perinatal hydrocephalus. Hydrocephalus of prenatal origin, which may be hereditary, is likely to be discovered more frequently and earlier in gestation with ultrasonic diagnosis. It is suggested that each case be analyzed according to a protocol which includes a full obstetric evaluation, complete genetic pedigree, determination of familial cranial size, and complete neuropathologic study. Destruction of the aqueductal area may deprive the investigator of much of the information needed for the thorough study of the case which is vital to counsel the parents concerning future pregnancies.", "PMID": 961754}
{"id": "pubmed23n0032_4628", "title": "The effect of L-dopa and chlorpromazine on prolactin and growth hormone secretion in normal women.", "content": "The time course of simultaneous changes in prolactin (PRL) and growth hormone secretion in response to a single dose of L-dopa and chlorpromazine was determined in normal women. L-Dopa induced greater, but shorter (30 minutes), growth hormone release than concomitant suppression of PRL secretion. The PRL peak following chlorpromazine occurred at the same time as the nadir of PRL after L-dopa (3.5 hours). The quantity of PRL release inhibited by L-dopa equaled the amount of PRL secretion during the period of rebound, suggesting L-dopa inhibits PRL release, but not synthesis, by the pituitary.", "contents": "The effect of L-dopa and chlorpromazine on prolactin and growth hormone secretion in normal women. The time course of simultaneous changes in prolactin (PRL) and growth hormone secretion in response to a single dose of L-dopa and chlorpromazine was determined in normal women. L-Dopa induced greater, but shorter (30 minutes), growth hormone release than concomitant suppression of PRL secretion. The PRL peak following chlorpromazine occurred at the same time as the nadir of PRL after L-dopa (3.5 hours). The quantity of PRL release inhibited by L-dopa equaled the amount of PRL secretion during the period of rebound, suggesting L-dopa inhibits PRL release, but not synthesis, by the pituitary.", "PMID": 961756}
{"id": "pubmed23n0032_4629", "title": "Vasomotor symptoms, serum estrogens, and gonadotropin levels in surgical menopause.", "content": "Hormonal parameters of young women who developed vasomotor symptoms in the immediate postoperative period following castration are compared to those who remained asymptomatic. Only 37.5 per cent of 16 premenopausal women developed \"hot flushes\" after operation. Perimenopausal women with vasomotor symptoms and elevated follicle-stimulating hormone levels demonstrated normal luteinizing hormone and estrogen values preoperatively. There were no statistically significant differences in total serum estrogen, follicle-stimulating hormone, or luteinizing hormone concentrations between the group of patients with symptoms and the group withoyt symptoms. The results of the study indicate that rising gonadotropin or declining estrogen values appear to have no direct correlation to the onset of vasomotor symptoms in the immediate postoperative period. Thus, the precise etiology of the \"hot flush\" remains to be elucidated.", "contents": "Vasomotor symptoms, serum estrogens, and gonadotropin levels in surgical menopause. Hormonal parameters of young women who developed vasomotor symptoms in the immediate postoperative period following castration are compared to those who remained asymptomatic. Only 37.5 per cent of 16 premenopausal women developed \"hot flushes\" after operation. Perimenopausal women with vasomotor symptoms and elevated follicle-stimulating hormone levels demonstrated normal luteinizing hormone and estrogen values preoperatively. There were no statistically significant differences in total serum estrogen, follicle-stimulating hormone, or luteinizing hormone concentrations between the group of patients with symptoms and the group withoyt symptoms. The results of the study indicate that rising gonadotropin or declining estrogen values appear to have no direct correlation to the onset of vasomotor symptoms in the immediate postoperative period. Thus, the precise etiology of the \"hot flush\" remains to be elucidated.", "PMID": 961757}
{"id": "pubmed23n0032_4630", "title": "Scanning and transmission electron microscopic studies of vaginal adenosis and the cervical transformation zone in progeny exposed in utero to diethylstilbestrol.", "content": "Much attention has recently been focused on vaginal adenosis and other cervical and vaginal alterations occurring in female offspring exposed to diethylstilbestrol (DES) in utero. These alterations consist of heterotopic glandular epithelium in the vagina, a characteristic cervical transformation zone, and the frequent presence of a cervical collar, or hood. It is believed that these changes occur during embryonic differentiation of the lower genital tract following failure of squamous epithelium to migrate in a cephalad direction to replace columnar epithelium which normally lines the vagina and ectocervix. A number of cases clear-cell carcinoma have occurred in DES-exposed girls who also had adenosis. In an attempt to characterize the cellular populations of these areas of adenosis, we have made an ultrastructural study which is herein reported.", "contents": "Scanning and transmission electron microscopic studies of vaginal adenosis and the cervical transformation zone in progeny exposed in utero to diethylstilbestrol. Much attention has recently been focused on vaginal adenosis and other cervical and vaginal alterations occurring in female offspring exposed to diethylstilbestrol (DES) in utero. These alterations consist of heterotopic glandular epithelium in the vagina, a characteristic cervical transformation zone, and the frequent presence of a cervical collar, or hood. It is believed that these changes occur during embryonic differentiation of the lower genital tract following failure of squamous epithelium to migrate in a cephalad direction to replace columnar epithelium which normally lines the vagina and ectocervix. A number of cases clear-cell carcinoma have occurred in DES-exposed girls who also had adenosis. In an attempt to characterize the cellular populations of these areas of adenosis, we have made an ultrastructural study which is herein reported.", "PMID": 961758}
{"id": "pubmed23n0032_4631", "title": "Response of human uterine arteries to local anesthetics.", "content": "The in vitro effects of local anesthetics and norepinephrine upon strips of early gestation and term pregnancy uterine arteries were studied in eight cases. In another case, the effect upon uterine veins was studied with a standard organ bath used to record isometric contractions. Histologic preparations were made to verify the type of vessel studied. An artery obtained from an eight-week gestation did not respond to either lidocaine or mepivacaine. All other arterial specimens (radial and helicoidal strips) responded with slow, rising, strong contractions to diluted concentrations of both of these substances. Likewise they responded with rapid contractions when exposed to norepinephrine. Alpha blockers were unable to prevent the contractions triggered by the local anesthetics. The vein specimens did not respond to local anesthetics but contracted when stimulated by norepinephrine. Based on these observations and after brief review of some hypotheses advanced to explain post-paracervical anesthesia fetal bradycardia, it is postulated that this bradycardia is probably due to uterine artery spasm, causing decreased intervillous space blood flow and fetal hypoxia.", "contents": "Response of human uterine arteries to local anesthetics. The in vitro effects of local anesthetics and norepinephrine upon strips of early gestation and term pregnancy uterine arteries were studied in eight cases. In another case, the effect upon uterine veins was studied with a standard organ bath used to record isometric contractions. Histologic preparations were made to verify the type of vessel studied. An artery obtained from an eight-week gestation did not respond to either lidocaine or mepivacaine. All other arterial specimens (radial and helicoidal strips) responded with slow, rising, strong contractions to diluted concentrations of both of these substances. Likewise they responded with rapid contractions when exposed to norepinephrine. Alpha blockers were unable to prevent the contractions triggered by the local anesthetics. The vein specimens did not respond to local anesthetics but contracted when stimulated by norepinephrine. Based on these observations and after brief review of some hypotheses advanced to explain post-paracervical anesthesia fetal bradycardia, it is postulated that this bradycardia is probably due to uterine artery spasm, causing decreased intervillous space blood flow and fetal hypoxia.", "PMID": 961759}
{"id": "pubmed23n0032_4632", "title": "Reduction of the stretch modulus of human cervical tissue by prostaglandin E2.", "content": "Samples of cervical tissue were obtained from women immediately following term delivery from spontaneous labor or oral PGE2-induced labor and from a small nunber of nonpregnant women in the reproductive years undergoing hysterectomy for benign gynecologic disease. The measured strips were placed in a well-oxygenated bath at 37 degrees C. containing Ringer's solution and stretched at a constant rate while continuously recording length and tension. The data were converted to stress-strain diagrams. Each curve contained a long linear portion which allowed one to compute a stretch modulus for each sample. A total of 71 strips from 23 patients was used. The average stretch modulus from the oral PGE2-induced patients was significantly lower than the spontaneous labor group (p less than 0.005), which, in turn, was significantly lower than the stretch modulus of the nonpregnant cervical tissue. It was also noted that the yield point was lower in the PGE2 series when compared to the spontaneous labor series (p less than 0.05). The effect of the PGE2 at a bath concentration of 10(-5) to 10(-6)Gm. per cubic centimeter was to materially reduce the stretch modulus within 5 to 15 minutes of the drug addition in both the PGE2-induced and spontaneous labor series. The results of these experiments indicate that PGE2 has the effect of reducing cervial stiffness.", "contents": "Reduction of the stretch modulus of human cervical tissue by prostaglandin E2. Samples of cervical tissue were obtained from women immediately following term delivery from spontaneous labor or oral PGE2-induced labor and from a small nunber of nonpregnant women in the reproductive years undergoing hysterectomy for benign gynecologic disease. The measured strips were placed in a well-oxygenated bath at 37 degrees C. containing Ringer's solution and stretched at a constant rate while continuously recording length and tension. The data were converted to stress-strain diagrams. Each curve contained a long linear portion which allowed one to compute a stretch modulus for each sample. A total of 71 strips from 23 patients was used. The average stretch modulus from the oral PGE2-induced patients was significantly lower than the spontaneous labor group (p less than 0.005), which, in turn, was significantly lower than the stretch modulus of the nonpregnant cervical tissue. It was also noted that the yield point was lower in the PGE2 series when compared to the spontaneous labor series (p less than 0.05). The effect of the PGE2 at a bath concentration of 10(-5) to 10(-6)Gm. per cubic centimeter was to materially reduce the stretch modulus within 5 to 15 minutes of the drug addition in both the PGE2-induced and spontaneous labor series. The results of these experiments indicate that PGE2 has the effect of reducing cervial stiffness.", "PMID": 961760}
{"id": "pubmed23n0032_4633", "title": "Spontaneous contractility and the response of the human uterine cervix to prostaglandins F2alpha and E2 during the menstrual cycle.", "content": "The effect of prostaglandins E2 and F2alpha on the contractility of the uterine cervix was investigated in nonpregnant women throughout the menstrual cycle. PGF2alpha was found stimulatory to the cervix at all phases of the menstrual cycle, whereas PGE2 was inhibitory. The relaxing effect of PGE2 was most marked during midcycle.", "contents": "Spontaneous contractility and the response of the human uterine cervix to prostaglandins F2alpha and E2 during the menstrual cycle. The effect of prostaglandins E2 and F2alpha on the contractility of the uterine cervix was investigated in nonpregnant women throughout the menstrual cycle. PGF2alpha was found stimulatory to the cervix at all phases of the menstrual cycle, whereas PGE2 was inhibitory. The relaxing effect of PGE2 was most marked during midcycle.", "PMID": 961761}
{"id": "pubmed23n0032_4634", "title": "Abnormal adolescent primiparous pregnancy: association of race, human chorionic somatomammotropin production, and smoking.", "content": "Abnormal pregnancies (33 per cent) were studied in 417 consecutive, unmarried, primiparous women, age 12 to 18 years (black, 54 per cent; white, 46 per cent). Black patients had no increase in abnormal pregnancies but had more infants weighting less than 2,500 grams (p less than 0.01). Black patients with pre-eclampsia gained less weight than white patients (24 versus 34 pounds, p less than 0.05) and had smaller infants (2,834 versus 3,316 grams). In white patients, the mean age at menarche was earlier in women who lost their infants (11.0 versus 12.7 years; p less than 0.01). Smoking was more prevalent in normal pregnancies (47 per cent) than abnormal pregnancies (38 per cent). Spontaneous abortion and fetal loss occurred more often in smokers (p less than 0.01). White women smoked more than blacks (60 versus 37 per cent). In mothers of infants weighting less than 2,500 grams, there were fewer smokers. Unexpectedly, smokers had less hypertension and pre-eclampsia. Human chorionic somatomammotropin values correlated with infant size. Levels of less than 4 mug per milliliter after 30 weeks' gestation were identical in normal and abnormal pregnancies (4.4 per cent). They were associated with small infants but no fetal deaths and did not predict outcome of pregnancy.", "contents": "Abnormal adolescent primiparous pregnancy: association of race, human chorionic somatomammotropin production, and smoking. Abnormal pregnancies (33 per cent) were studied in 417 consecutive, unmarried, primiparous women, age 12 to 18 years (black, 54 per cent; white, 46 per cent). Black patients had no increase in abnormal pregnancies but had more infants weighting less than 2,500 grams (p less than 0.01). Black patients with pre-eclampsia gained less weight than white patients (24 versus 34 pounds, p less than 0.05) and had smaller infants (2,834 versus 3,316 grams). In white patients, the mean age at menarche was earlier in women who lost their infants (11.0 versus 12.7 years; p less than 0.01). Smoking was more prevalent in normal pregnancies (47 per cent) than abnormal pregnancies (38 per cent). Spontaneous abortion and fetal loss occurred more often in smokers (p less than 0.01). White women smoked more than blacks (60 versus 37 per cent). In mothers of infants weighting less than 2,500 grams, there were fewer smokers. Unexpectedly, smokers had less hypertension and pre-eclampsia. Human chorionic somatomammotropin values correlated with infant size. Levels of less than 4 mug per milliliter after 30 weeks' gestation were identical in normal and abnormal pregnancies (4.4 per cent). They were associated with small infants but no fetal deaths and did not predict outcome of pregnancy.", "PMID": 961762}
{"id": "pubmed23n0032_4635", "title": "Measurement of the pre-ejection period during labor with the use of arterial pulse time.", "content": "A new fetal parameter, the arterial pulse time, is described. It is the time from the onset of the fetal electrocardiographic recording to the arrival of the arterial pulse wave recorded at the fetal head. Since arterial pulse time differs from the pre-ejection period by only an apparently constant value, it is proposed as a potential method for obtaining the pre-ejection period during labor.", "contents": "Measurement of the pre-ejection period during labor with the use of arterial pulse time. A new fetal parameter, the arterial pulse time, is described. It is the time from the onset of the fetal electrocardiographic recording to the arrival of the arterial pulse wave recorded at the fetal head. Since arterial pulse time differs from the pre-ejection period by only an apparently constant value, it is proposed as a potential method for obtaining the pre-ejection period during labor.", "PMID": 961763}
{"id": "pubmed23n0032_4636", "title": "Observations on maternal oxytocin release during human labor and the effect of intravenous alcohol administration.", "content": "Serial blood samples were obtained from 97 women in spontaneous labor, and oxytocin levels were determined by a sensitive and highly specific radioimmunoassay. The following observations were made: (1) The hormone is released in a series of \"spurts\"; (2) the peak levels achieved are usually 2 to 5 muU per milliliter and never exceed 12.5 muU per milliliter; (3) the frequency of spurt release increases as labor progresses, reaching a maximum in the second stage; (4) the frequency of spurt release is reduced during intravenous infusion of alcohol at levels which produce no obvious effect on uterine activity.", "contents": "Observations on maternal oxytocin release during human labor and the effect of intravenous alcohol administration. Serial blood samples were obtained from 97 women in spontaneous labor, and oxytocin levels were determined by a sensitive and highly specific radioimmunoassay. The following observations were made: (1) The hormone is released in a series of \"spurts\"; (2) the peak levels achieved are usually 2 to 5 muU per milliliter and never exceed 12.5 muU per milliliter; (3) the frequency of spurt release increases as labor progresses, reaching a maximum in the second stage; (4) the frequency of spurt release is reduced during intravenous infusion of alcohol at levels which produce no obvious effect on uterine activity.", "PMID": 961764}
{"id": "pubmed23n0032_4637", "title": "Needle puncture of fetus: a complication of second-trimester amniocentesis.", "content": "Needle puncture of the fetus has rarely been reported with midtrimester amniocentesis. This paper contains the report of five cases of needle scars in infants born after second-trimester amniocentesis for prenatal diagnosis of fetal genetic disorders. Since this complication may be more frequent than has been previously believed, there is the possibility that damage to the fetus may occur. It is suggested that the products of all abortions and all live-born and stillborn infants delivered following amniocentesis should be examined for evidence of injury.", "contents": "Needle puncture of fetus: a complication of second-trimester amniocentesis. Needle puncture of the fetus has rarely been reported with midtrimester amniocentesis. This paper contains the report of five cases of needle scars in infants born after second-trimester amniocentesis for prenatal diagnosis of fetal genetic disorders. Since this complication may be more frequent than has been previously believed, there is the possibility that damage to the fetus may occur. It is suggested that the products of all abortions and all live-born and stillborn infants delivered following amniocentesis should be examined for evidence of injury.", "PMID": 961765}
{"id": "pubmed23n0032_4638", "title": "In vitro perfusion of human placenta. V. Oxygen consumption.", "content": "02 consumption by human term placenta has been measured during perfusion in vitro, with a technique that permits perfusion of the intervillous space as well as the umbilical circulation. The rate at which 02 was consumed was directly related to the rate at which it was supplied, within the limits studied. Previous estimates of 02 consumption yielded figures approximating 3 ml. per minute per kilogram of placenta, but these estimates were attained under conditions of limited oxygen supply. When oxygen was delivered to the placenta at rates believed to be those achieved in vivo, oxygen consumption approximated 10 ml. per minute per kilogram. This rate of 02 consumption is similar to those obtained from studies of animal placentas in vivo and is more consistent with that of an active metabolic organ. If one were to accept estimates of 02 consumption by the human fetus in utero, the present studies indicate that approximately one fifth of maternal oxygen supplied to the conceptus is diverted to support placental metabolism.", "contents": "In vitro perfusion of human placenta. V. Oxygen consumption. 02 consumption by human term placenta has been measured during perfusion in vitro, with a technique that permits perfusion of the intervillous space as well as the umbilical circulation. The rate at which 02 was consumed was directly related to the rate at which it was supplied, within the limits studied. Previous estimates of 02 consumption yielded figures approximating 3 ml. per minute per kilogram of placenta, but these estimates were attained under conditions of limited oxygen supply. When oxygen was delivered to the placenta at rates believed to be those achieved in vivo, oxygen consumption approximated 10 ml. per minute per kilogram. This rate of 02 consumption is similar to those obtained from studies of animal placentas in vivo and is more consistent with that of an active metabolic organ. If one were to accept estimates of 02 consumption by the human fetus in utero, the present studies indicate that approximately one fifth of maternal oxygen supplied to the conceptus is diverted to support placental metabolism.", "PMID": 961766}
{"id": "pubmed23n0032_4639", "title": "The significance of fetal electrocardiography in the diagnosis of intrauterine bradyarrhythmia.", "content": "From 11 cases of fetal bradycardia diagnosed by monitoring of 130 fetal electrocardiograms (ECG's) in high-risk pregnancies we have presented our experience in three selected cases of fetal bradyarrthythmia. Case 1 revealed on ECG blocked atrial premature beats simulating an extreme sinus bradycardia sequentially followed by conducted atrial premature beats. In case 2 we diagnosed ventricular premature beats in the form of persistent bigeminy which was controlled by intravenous propranolol. The last case illustrated the phenomenon of aberrant ventricular conduction known to occur in adult cardiology. The electrophysiologic basis of the variable arrhythmias was discussed. Detailed analysis of repeated direct fetal ECG's provided us with the diagnosis and understanding of the electrophysiologic mechanisms underlying the rhythm disturbances. This consequently determined the pharmacologic therapy and the obstetric approach relevant to each case. We have shown that by direct fetal electrocardiography it is possible to analyze accurately the rhythm disturbances. Persistent fetal bradycardia does not always signify fetal distress. We hope that this will lead to closer teamwork between the obstetrician and the cardiologist which will give an impetus to the future development of \"fetal cardiology,\" thereby enhancing our understanding of the electrophysiology of the fetal heart.", "contents": "The significance of fetal electrocardiography in the diagnosis of intrauterine bradyarrhythmia. From 11 cases of fetal bradycardia diagnosed by monitoring of 130 fetal electrocardiograms (ECG's) in high-risk pregnancies we have presented our experience in three selected cases of fetal bradyarrthythmia. Case 1 revealed on ECG blocked atrial premature beats simulating an extreme sinus bradycardia sequentially followed by conducted atrial premature beats. In case 2 we diagnosed ventricular premature beats in the form of persistent bigeminy which was controlled by intravenous propranolol. The last case illustrated the phenomenon of aberrant ventricular conduction known to occur in adult cardiology. The electrophysiologic basis of the variable arrhythmias was discussed. Detailed analysis of repeated direct fetal ECG's provided us with the diagnosis and understanding of the electrophysiologic mechanisms underlying the rhythm disturbances. This consequently determined the pharmacologic therapy and the obstetric approach relevant to each case. We have shown that by direct fetal electrocardiography it is possible to analyze accurately the rhythm disturbances. Persistent fetal bradycardia does not always signify fetal distress. We hope that this will lead to closer teamwork between the obstetrician and the cardiologist which will give an impetus to the future development of \"fetal cardiology,\" thereby enhancing our understanding of the electrophysiology of the fetal heart.", "PMID": 961767}
{"id": "pubmed23n0032_4640", "title": "Moderate fetal bradycardia.", "content": "Fetal heart rates in the range of 100 to 119 beats per minute have been defined as moderate bradycardia. Twenty-five of 1,386 patients monitored during a 1 year period have manifested persistent moderate bradycardia. Moderate bradycardia is apparently related to fetal head compression, often in situations of relative cephalopelvic disproportion. It is alleviated by rotation of the vertex and does not appear to adversely affect the newborn infant.", "contents": "Moderate fetal bradycardia. Fetal heart rates in the range of 100 to 119 beats per minute have been defined as moderate bradycardia. Twenty-five of 1,386 patients monitored during a 1 year period have manifested persistent moderate bradycardia. Moderate bradycardia is apparently related to fetal head compression, often in situations of relative cephalopelvic disproportion. It is alleviated by rotation of the vertex and does not appear to adversely affect the newborn infant.", "PMID": 961768}
{"id": "pubmed23n0032_4641", "title": "Human uterine artery responses to lidocaine.", "content": "Uterine arteries from pregnant and nonpregnant patients were studied to define their responses to lidocaine. Although local anesthetics usually are considered vasodilators, these data indicate that the human uterine artery constricts when exposed to 1,000 mug of lidocaine in vitro. Furthermore, those arteries from pregnant patients exhibited significantly greater responses than did those from nonpregnant patients.", "contents": "Human uterine artery responses to lidocaine. Uterine arteries from pregnant and nonpregnant patients were studied to define their responses to lidocaine. Although local anesthetics usually are considered vasodilators, these data indicate that the human uterine artery constricts when exposed to 1,000 mug of lidocaine in vitro. Furthermore, those arteries from pregnant patients exhibited significantly greater responses than did those from nonpregnant patients.", "PMID": 961778}
{"id": "pubmed23n0032_4642", "title": "Repeat aborters.", "content": "In an attempt to learn more about the phenomenon of repeat abortions, 116 women seeking a repeat abortion were compared in various ways with three groups of women not seeking a repeat abortion but otherwise similar. From this study it is apparent that postabortion women become, over time, less persistent users of contraception than sexually active nonpregnant women. However, they are more likely to use contraception continually than are women seeking a first abortion. The abortion experience, with or without concurrent family-planning guidance, does not create universally sufficient long-lasting motivation to use of contraception. Some other factors which might contribute to this motivation are presented.", "contents": "Repeat aborters. In an attempt to learn more about the phenomenon of repeat abortions, 116 women seeking a repeat abortion were compared in various ways with three groups of women not seeking a repeat abortion but otherwise similar. From this study it is apparent that postabortion women become, over time, less persistent users of contraception than sexually active nonpregnant women. However, they are more likely to use contraception continually than are women seeking a first abortion. The abortion experience, with or without concurrent family-planning guidance, does not create universally sufficient long-lasting motivation to use of contraception. Some other factors which might contribute to this motivation are presented.", "PMID": 961779}
{"id": "pubmed23n0032_4643", "title": "Fetal immune response following prematurely ruptured membranes.", "content": "Concentrations of immunoglobulins (Ig)A1, and IgA2, IgD, IgE, IgG, and IgM have been determined in cord blood, amniotic fluid, and maternal serum in a group of patients with a history of prematurely ruptured membranes (PRM) prior to the onset of labor and in a control group of patients undergoing normal delivery and without a history of infection during pregnancy. IgA and IgD were determined by sensitive hemagglutination-inhibition tests; IgG and IgM, by radial immunodiffusion; IgE, by a radioimmunoassay. There was evidence for an immune response in 10 of 16 cases of PRM: five of 16 had increased IgA but normal IgM; three of 16 had increased IgA and IgM; two of 16 had high IgM and normal IgA in cord blood. In patients with significantly increased levels of either IgA or IgM or both, there was a decreased level of IgD. These changes are most likely the result of the immune response to ascending infection from the maternal genitals. The sensitive testing method employed could demonstrate the presence of IgD in 53 per cent of normal cord blood samples and 72 per cent of amniotic fluid samples obtained at term. IgE was found in all normal cord blood and amniotic fluid samples tested. By concentrating the amniotic fluid up to 180-fold, IgM was demonstrated in all normal samples tested. The potential importance of IgA determinations in cord blood in addition to IgM determination for detection of intrauterine infections is stressed.", "contents": "Fetal immune response following prematurely ruptured membranes. Concentrations of immunoglobulins (Ig)A1, and IgA2, IgD, IgE, IgG, and IgM have been determined in cord blood, amniotic fluid, and maternal serum in a group of patients with a history of prematurely ruptured membranes (PRM) prior to the onset of labor and in a control group of patients undergoing normal delivery and without a history of infection during pregnancy. IgA and IgD were determined by sensitive hemagglutination-inhibition tests; IgG and IgM, by radial immunodiffusion; IgE, by a radioimmunoassay. There was evidence for an immune response in 10 of 16 cases of PRM: five of 16 had increased IgA but normal IgM; three of 16 had increased IgA and IgM; two of 16 had high IgM and normal IgA in cord blood. In patients with significantly increased levels of either IgA or IgM or both, there was a decreased level of IgD. These changes are most likely the result of the immune response to ascending infection from the maternal genitals. The sensitive testing method employed could demonstrate the presence of IgD in 53 per cent of normal cord blood samples and 72 per cent of amniotic fluid samples obtained at term. IgE was found in all normal cord blood and amniotic fluid samples tested. By concentrating the amniotic fluid up to 180-fold, IgM was demonstrated in all normal samples tested. The potential importance of IgA determinations in cord blood in addition to IgM determination for detection of intrauterine infections is stressed.", "PMID": 961780}
{"id": "pubmed23n0032_4644", "title": "Abnormality in metabolism of 15N-labeled nitrate in toxemia.", "content": "The urinary excretion of 15N-labeled ammonium nitrate was studied during the last few weeks in patients with normal pregnancy and in those with toxemia. Marked diminution of the 15N label was found consistently in patients with toxemia in the urinary fraction consisting of nitrogenous compounds other than urea and ammonia. The possible significance of this finding is discussed.", "contents": "Abnormality in metabolism of 15N-labeled nitrate in toxemia. The urinary excretion of 15N-labeled ammonium nitrate was studied during the last few weeks in patients with normal pregnancy and in those with toxemia. Marked diminution of the 15N label was found consistently in patients with toxemia in the urinary fraction consisting of nitrogenous compounds other than urea and ammonia. The possible significance of this finding is discussed.", "PMID": 961781}
{"id": "pubmed23n0032_4645", "title": "Effect of ritodrine infusion on uterine and umbilical blood flow in pregnant sheep.", "content": "The effect of ritodrine upon uterine artery blood flow (UtBF) and umbilical vein blood flow (UmBF) was investigated in near-term chronic sheep preparations. During intravenous ritodrine infusions to the ewe in sequential dose rates from 100 to 800 mug per minute, UtBF fell progressively to 43 per cent below control levels and mean maternal arterial pressure (MMAP) declined 20 per cent. During constant infusions of 100, 400, or 800 mug per minute of ritodrine to the ewe for 120 minutes,, UtBF decreased 10, 37, and 31 per cent, respectively, and MMAP decreased 6, 20 and 25 per cent respectively. Dose-related maternal tachycardia and hyperglycemia occurred. There were no significant changes in UmBF, mean fetal arterial pressure, or fetal heart rate. During all infusions, maternal and fetal arterial pH, PCO2, and PO2 remained within normal physiologic limits. Simultaneous infusion of ritodrine (400 mug per minute) and propranolol (100 mug per minute) blocked the maternal tachycardia, but decreases in UtBF, MMAP, and UmBF were observed. Ritodrine infusions to the fetus (25 mug per minute) resulted in fetal tachycardia and a variable increase in UmBF.", "contents": "Effect of ritodrine infusion on uterine and umbilical blood flow in pregnant sheep. The effect of ritodrine upon uterine artery blood flow (UtBF) and umbilical vein blood flow (UmBF) was investigated in near-term chronic sheep preparations. During intravenous ritodrine infusions to the ewe in sequential dose rates from 100 to 800 mug per minute, UtBF fell progressively to 43 per cent below control levels and mean maternal arterial pressure (MMAP) declined 20 per cent. During constant infusions of 100, 400, or 800 mug per minute of ritodrine to the ewe for 120 minutes,, UtBF decreased 10, 37, and 31 per cent, respectively, and MMAP decreased 6, 20 and 25 per cent respectively. Dose-related maternal tachycardia and hyperglycemia occurred. There were no significant changes in UmBF, mean fetal arterial pressure, or fetal heart rate. During all infusions, maternal and fetal arterial pH, PCO2, and PO2 remained within normal physiologic limits. Simultaneous infusion of ritodrine (400 mug per minute) and propranolol (100 mug per minute) blocked the maternal tachycardia, but decreases in UtBF, MMAP, and UmBF were observed. Ritodrine infusions to the fetus (25 mug per minute) resulted in fetal tachycardia and a variable increase in UmBF.", "PMID": 961782}
{"id": "pubmed23n0032_4646", "title": "Placental cultures for cytogenetic assessment in saline-aborted fetuses.", "content": "Confirmation of cytogenetically abnormal fetuses following saline abortion has been shown to be possible with the placenta as the source of viable fetal cells. The method is described in detail. In one third of cultures, only female cells were present. Differentiation between maternal and female fetal tissue when no numerical or structural cytogenetic disorder is present requires detailed analysis of fluorescently stained chromosomes for polymorphisms.", "contents": "Placental cultures for cytogenetic assessment in saline-aborted fetuses. Confirmation of cytogenetically abnormal fetuses following saline abortion has been shown to be possible with the placenta as the source of viable fetal cells. The method is described in detail. In one third of cultures, only female cells were present. Differentiation between maternal and female fetal tissue when no numerical or structural cytogenetic disorder is present requires detailed analysis of fluorescently stained chromosomes for polymorphisms.", "PMID": 961783}
{"id": "pubmed23n0032_4647", "title": "Incorporation of 14C-1-palmitate into rabbit fetal lipids in vivo.", "content": "14C-1-palmitate was injected into rabbit fetuses in utero. Fetal liver, blood, carcass, and placenta collected after 15, 60, 180, and 360 minutes were analyzed for radioactivity in lipid classes. Incorporation differed with tissue and lipid. Highest total early specific activity was detected in plasma but later liver displayed the most extensive incorporation. Phospholipid incorporation was more rapid in all tissues than that of other glycerides. Plasma triglycerides did not display any radioactivity for 3 hours. Results point to multicentric origin of plasma lipids. Ready utilization of the palmitate indicates that maternal fatty acid transfer plays an important role in fetal lipid hemeostasis.", "contents": "Incorporation of 14C-1-palmitate into rabbit fetal lipids in vivo. 14C-1-palmitate was injected into rabbit fetuses in utero. Fetal liver, blood, carcass, and placenta collected after 15, 60, 180, and 360 minutes were analyzed for radioactivity in lipid classes. Incorporation differed with tissue and lipid. Highest total early specific activity was detected in plasma but later liver displayed the most extensive incorporation. Phospholipid incorporation was more rapid in all tissues than that of other glycerides. Plasma triglycerides did not display any radioactivity for 3 hours. Results point to multicentric origin of plasma lipids. Ready utilization of the palmitate indicates that maternal fatty acid transfer plays an important role in fetal lipid hemeostasis.", "PMID": 961784}
{"id": "pubmed23n0032_4648", "title": "Fallopian tube and ovarian hydrocarbons.", "content": "Hydrocarbons of the human ovary and Fallopian tube have been studied by temperature-programmed gas chromatography. The major compounds present are a series of n-alkanes (C16 to C40), with the addition of iso and anteiso hydrocarbons. The human ovary and Fallopian tube hydrocarbons did not show significant changes during the menstrual cycle.", "contents": "Fallopian tube and ovarian hydrocarbons. Hydrocarbons of the human ovary and Fallopian tube have been studied by temperature-programmed gas chromatography. The major compounds present are a series of n-alkanes (C16 to C40), with the addition of iso and anteiso hydrocarbons. The human ovary and Fallopian tube hydrocarbons did not show significant changes during the menstrual cycle.", "PMID": 961785}
{"id": "pubmed23n0032_4649", "title": "Progestational activity of danazol in the human female subject.", "content": "A progestational action of Danazol has not been reported in the human subject. Danazol administration for 1 month suppressed serum FSH and estradiol values, and the midcycle LH surge; ovulation promptly resumed after treatment. Administration to patients with polycystic ovarian disease resulted in increased LH values, elevated basal temperature, secretory findings in the endometrial biopsy, and menses. Ovarian biopsy did not document corpus luteum formation, and plasma progesterone did not rise. Administration of Danazol to an ovariectomized patients pretreated with estrogen resulted in an atypical endometrial secretory change. Clinical responses to Danazol may in part be explained by a progestational-like effect of the drug.", "contents": "Progestational activity of danazol in the human female subject. A progestational action of Danazol has not been reported in the human subject. Danazol administration for 1 month suppressed serum FSH and estradiol values, and the midcycle LH surge; ovulation promptly resumed after treatment. Administration to patients with polycystic ovarian disease resulted in increased LH values, elevated basal temperature, secretory findings in the endometrial biopsy, and menses. Ovarian biopsy did not document corpus luteum formation, and plasma progesterone did not rise. Administration of Danazol to an ovariectomized patients pretreated with estrogen resulted in an atypical endometrial secretory change. Clinical responses to Danazol may in part be explained by a progestational-like effect of the drug.", "PMID": 961786}
{"id": "pubmed23n0032_4650", "title": "The late stage of serpiginous (geographic) choroiditis.", "content": "Serpiginous and geographic choroiditis, one and the same disease, is characterized by episodic involvement of the pigment epithelium and choroid. Each new lesion passes through an acute phase into an atrophic and scarring one. We observed 20 patients, some with long-term follow-up; some had eventual widespread involvement of both posterior poles. There was no basis for the disease, and treatment did not affect its course. In the patients with long-term disease there was widespread atrophy of the choroid and pigment epithelium and variable amounts of pigment clumping and subretinal fibrous tissue deposition. In nine patients discrete peripheral patches of atrophy were also found. In some eyes the atrophy at the posterior pole had become so confluent that the condition could have been mistaken superficially for a primary choroidal dystrophy. The uninvolved areas appeared to be normal. The electroretinogram and electrooculogram were only affected when extensive disease was present.", "contents": "The late stage of serpiginous (geographic) choroiditis. Serpiginous and geographic choroiditis, one and the same disease, is characterized by episodic involvement of the pigment epithelium and choroid. Each new lesion passes through an acute phase into an atrophic and scarring one. We observed 20 patients, some with long-term follow-up; some had eventual widespread involvement of both posterior poles. There was no basis for the disease, and treatment did not affect its course. In the patients with long-term disease there was widespread atrophy of the choroid and pigment epithelium and variable amounts of pigment clumping and subretinal fibrous tissue deposition. In nine patients discrete peripheral patches of atrophy were also found. In some eyes the atrophy at the posterior pole had become so confluent that the condition could have been mistaken superficially for a primary choroidal dystrophy. The uninvolved areas appeared to be normal. The electroretinogram and electrooculogram were only affected when extensive disease was present.", "PMID": 961787}
{"id": "pubmed23n0032_4651", "title": "External buckling without drainage for selected detachments in aphakic eyes.", "content": "Of 325 patients with retinal detachments, 110 patients (34%) had aphakic eyes. These detachments were subdivided into three types based on their clinical appearance during indirect ophthalmoscopy and slit-lamp biomicroscopy. Fifty-two patients (47%) had aphakic detachments (Type 3), categorized by small tears due to traction along a prominent posterior or vitreous base and the absence of visible retinal degeneration. Fifty of the 52 patients in this group were operated on by using a modification of the nondrainage procedure developed by Custodis, and employing cryosurgical coagulation and an external encircling buckle using a 3-mm silicone sponge. The sponge was secured beneath the retinal tear, and its length was shortened to produce a moderately elevated buckle. Although 50% of retinal tears were open at the end of the operation, 60% of all eyes operated on without drainage reattached in 16 hours, 90% in one week, and the remainder in two weeks. Visual acuity of 70% of these patients was 6/15 (20/50) or better when tested six months after surgery. The operation did not wall away peripheral fluid, but closed retinal tears completely, reduced circumferential vitreous traction, and avoided drainage of subretinal fluid.", "contents": "External buckling without drainage for selected detachments in aphakic eyes. Of 325 patients with retinal detachments, 110 patients (34%) had aphakic eyes. These detachments were subdivided into three types based on their clinical appearance during indirect ophthalmoscopy and slit-lamp biomicroscopy. Fifty-two patients (47%) had aphakic detachments (Type 3), categorized by small tears due to traction along a prominent posterior or vitreous base and the absence of visible retinal degeneration. Fifty of the 52 patients in this group were operated on by using a modification of the nondrainage procedure developed by Custodis, and employing cryosurgical coagulation and an external encircling buckle using a 3-mm silicone sponge. The sponge was secured beneath the retinal tear, and its length was shortened to produce a moderately elevated buckle. Although 50% of retinal tears were open at the end of the operation, 60% of all eyes operated on without drainage reattached in 16 hours, 90% in one week, and the remainder in two weeks. Visual acuity of 70% of these patients was 6/15 (20/50) or better when tested six months after surgery. The operation did not wall away peripheral fluid, but closed retinal tears completely, reduced circumferential vitreous traction, and avoided drainage of subretinal fluid.", "PMID": 961788}
{"id": "pubmed23n0032_4652", "title": "Melanocytic lesions of the eyelid skin.", "content": "We described the three histogenetic forms of cutaneous melanoma--lentigo maligna melanoma, superficial spreading melanoma, and nodular melanoma. The first two forms have a flat spreading component or radial growth phase in the epithelium as well as in the invasive or vertical growth phase. Formerly these two lesions may have been confused with one another. Nodular melanoma has only the vertical growth phase. Examples of lentigo maligna melanoma and superficial spreading melanoma were found in eyelid lesions involving both the skin and conjunctiva. The distinctive histopathology of the cutaneous lesion was retained in the conjunctiva.", "contents": "Melanocytic lesions of the eyelid skin. We described the three histogenetic forms of cutaneous melanoma--lentigo maligna melanoma, superficial spreading melanoma, and nodular melanoma. The first two forms have a flat spreading component or radial growth phase in the epithelium as well as in the invasive or vertical growth phase. Formerly these two lesions may have been confused with one another. Nodular melanoma has only the vertical growth phase. Examples of lentigo maligna melanoma and superficial spreading melanoma were found in eyelid lesions involving both the skin and conjunctiva. The distinctive histopathology of the cutaneous lesion was retained in the conjunctiva.", "PMID": 961789}
{"id": "pubmed23n0032_4653", "title": "Malignant melanomas of the conjunctiva.", "content": "A retrospective study of 23 conjunctival melanomas using the Clark classification revealed that the three most common forms of melanoma described in the skin--lentigo maligna melanoma (Hutchinson's freckle with melanoma), superficial spreading melanoma, and nodular melanoma--can be recognized in the conjunctiva. As in the skin, lentigo maligna melanoma appears to be associated with a good prognosis compared to the prognosis associated with superficial spreading melanoma. These two forms of melanoma are both associated with an intraepithelial stage and had previously been grouped under one designate, cancerous melanosis. They can be distinguished histologically although definite clinical differentiation will be determined in the future. Some melanomas with an intraepithelial stage, however, cannot be definitely classified. Relating all conjunctival cancerous melanoses to Hutchinson's melanotic freckle is no longer justified.", "contents": "Malignant melanomas of the conjunctiva. A retrospective study of 23 conjunctival melanomas using the Clark classification revealed that the three most common forms of melanoma described in the skin--lentigo maligna melanoma (Hutchinson's freckle with melanoma), superficial spreading melanoma, and nodular melanoma--can be recognized in the conjunctiva. As in the skin, lentigo maligna melanoma appears to be associated with a good prognosis compared to the prognosis associated with superficial spreading melanoma. These two forms of melanoma are both associated with an intraepithelial stage and had previously been grouped under one designate, cancerous melanosis. They can be distinguished histologically although definite clinical differentiation will be determined in the future. Some melanomas with an intraepithelial stage, however, cannot be definitely classified. Relating all conjunctival cancerous melanoses to Hutchinson's melanotic freckle is no longer justified.", "PMID": 961790}
{"id": "pubmed23n0032_4654", "title": "A histologic study of calcific and noncalcific band keratopathies.", "content": "One hundred five cases previously diagnosed as band keratopathy were reexamined. We found that band keratopathy was calcific noncalcific, or mixture of both. The noncalcific band was identified as elastotic degeneration of collagen, known clinically by various alternate terms. Cases of so-called reduplication of Bowman's membrane were reexamined, and many demonstrated production of a thick basement membrane of the corneal epithelium, with or without massive calcification. Identification of calcific-band keratopathy was made by transmission electron microscopy even when the tissue had been previously decalcified.", "contents": "A histologic study of calcific and noncalcific band keratopathies. One hundred five cases previously diagnosed as band keratopathy were reexamined. We found that band keratopathy was calcific noncalcific, or mixture of both. The noncalcific band was identified as elastotic degeneration of collagen, known clinically by various alternate terms. Cases of so-called reduplication of Bowman's membrane were reexamined, and many demonstrated production of a thick basement membrane of the corneal epithelium, with or without massive calcification. Identification of calcific-band keratopathy was made by transmission electron microscopy even when the tissue had been previously decalcified.", "PMID": 961791}
{"id": "pubmed23n0032_4655", "title": "Histologic studies of the vasculature of the anterior optic nerve.", "content": "Thirty-one normal human anterior optic nerves were studied in thin serial sections and their vessels stained by standard histologic stains and a new modification of the silver reticulin stain. The retrolaminar cribrosa was profusely supplied by centripetal and longitudinal vessels of pial origin; small branches of the central retinal artery were also seen. Longitudinal continuity of small vessels extended from the retrolaminar nerve to the retinal surface. This system freely anastomosed with three transverse systems; a significant anteriolar input from short posterior ciliary arteries in the sclera to the lamina cribrosa; smaller short posterior ciliary branches and occasional choroidal capillaries to the prelaminar portion; and branches derived from the central retinal artery in and around the disk. Vessels entering the prelaminar region at the level of the choroid were mainly derived from scleral branches of short posterior ciliary vessels entering through the border tissue of Elschnig, rather than from choroidal branches of ciliary arteries. Venous drainage for the anterior optic nerve was primarily through the central retinal vein, although alternate smaller paths to surrounding tissues were also identified.", "contents": "Histologic studies of the vasculature of the anterior optic nerve. Thirty-one normal human anterior optic nerves were studied in thin serial sections and their vessels stained by standard histologic stains and a new modification of the silver reticulin stain. The retrolaminar cribrosa was profusely supplied by centripetal and longitudinal vessels of pial origin; small branches of the central retinal artery were also seen. Longitudinal continuity of small vessels extended from the retrolaminar nerve to the retinal surface. This system freely anastomosed with three transverse systems; a significant anteriolar input from short posterior ciliary arteries in the sclera to the lamina cribrosa; smaller short posterior ciliary branches and occasional choroidal capillaries to the prelaminar portion; and branches derived from the central retinal artery in and around the disk. Vessels entering the prelaminar region at the level of the choroid were mainly derived from scleral branches of short posterior ciliary vessels entering through the border tissue of Elschnig, rather than from choroidal branches of ciliary arteries. Venous drainage for the anterior optic nerve was primarily through the central retinal vein, although alternate smaller paths to surrounding tissues were also identified.", "PMID": 961792}
{"id": "pubmed23n0032_4656", "title": "Electron microscopic study of human papilledema.", "content": "Papilledema associated with neoplastic invasion of the orbit in the optic disks of two patients was found and examined by light and electron microscopy. Pathologic changes in the optic nerve head and nerve included axonal degeneration and mild interstitial edema. Axonal swelling appeared to be the major factor in the overall increase in tissue volume of the optic nerve head. Vascular and perivascular glial alterations were nonspecific.", "contents": "Electron microscopic study of human papilledema. Papilledema associated with neoplastic invasion of the orbit in the optic disks of two patients was found and examined by light and electron microscopy. Pathologic changes in the optic nerve head and nerve included axonal degeneration and mild interstitial edema. Axonal swelling appeared to be the major factor in the overall increase in tissue volume of the optic nerve head. Vascular and perivascular glial alterations were nonspecific.", "PMID": 961793}
{"id": "pubmed23n0032_4657", "title": "Xerophthalmia and anterior segment blindness.", "content": "The prevalence of presumed vitamin A-related corneal scars among 5,589 preschool-age Haitian children ranged from 1.2 per 1,000 in the south, to almost 1% in the famine-afflicted north. These scars accounted for at least 45% of all corneal scars, and all bilateral corneal blindness encountered. Most lesions were acquired during the first three years of life. There was no variation by sex or ecology of the sample site. This country of 5.5 million inhabitants acquires 345 new surviving cases of vitamin A-related corneal destruction, over one fourth bilaterally blind, each year, although local ophthalmologists rarely encounter the disease and Bitot's spots were absent from our study population. Countrywide clinical prevalence surveys are the only unbiased means of determining the magnitude, severity, and geographic distribution of vitamin A-related corneal destruction, prerequisites for the design of public health prevention programs.", "contents": "Xerophthalmia and anterior segment blindness. The prevalence of presumed vitamin A-related corneal scars among 5,589 preschool-age Haitian children ranged from 1.2 per 1,000 in the south, to almost 1% in the famine-afflicted north. These scars accounted for at least 45% of all corneal scars, and all bilateral corneal blindness encountered. Most lesions were acquired during the first three years of life. There was no variation by sex or ecology of the sample site. This country of 5.5 million inhabitants acquires 345 new surviving cases of vitamin A-related corneal destruction, over one fourth bilaterally blind, each year, although local ophthalmologists rarely encounter the disease and Bitot's spots were absent from our study population. Countrywide clinical prevalence surveys are the only unbiased means of determining the magnitude, severity, and geographic distribution of vitamin A-related corneal destruction, prerequisites for the design of public health prevention programs.", "PMID": 961794}
{"id": "pubmed23n0032_4658", "title": "Thermokeratoplasty for keratoconus.", "content": "Thermokeratoplasty for keratoconus was performed in 29 eyes with follow-up periods ranging from less than four months to 24 months. Twelve patients obtained visual acuity better than 6/12 (20/40). Five patients required corneal transplantation.", "contents": "Thermokeratoplasty for keratoconus. Thermokeratoplasty for keratoconus was performed in 29 eyes with follow-up periods ranging from less than four months to 24 months. Twelve patients obtained visual acuity better than 6/12 (20/40). Five patients required corneal transplantation.", "PMID": 961795}
{"id": "pubmed23n0032_4659", "title": "Ultrastructure of keratoconus with healed hydrops.", "content": "The corneas of three patients with keratoconus were examined by light and electron microscopy within approximately four months after onset of severe hydrops. The rupture and detachment of Descemet's membrane resulted in formation of extensive ledges. New endothelium completely resurfaced the exposed posterior stroma and the corresponding anterior aspect of the ledges. The endothelium also regenerated considerable basement membrane. In one case, clustered endothelium near the free end of the ledge appeared to have undergone fibrous metaplasia with secretion of extensive fibrillar and basement membrane material.", "contents": "Ultrastructure of keratoconus with healed hydrops. The corneas of three patients with keratoconus were examined by light and electron microscopy within approximately four months after onset of severe hydrops. The rupture and detachment of Descemet's membrane resulted in formation of extensive ledges. New endothelium completely resurfaced the exposed posterior stroma and the corresponding anterior aspect of the ledges. The endothelium also regenerated considerable basement membrane. In one case, clustered endothelium near the free end of the ledge appeared to have undergone fibrous metaplasia with secretion of extensive fibrillar and basement membrane material.", "PMID": 961796}
{"id": "pubmed23n0032_4660", "title": "Ocular findings in mannosidosis.", "content": "Three patients with typical features of mannosidosis and deficiency of alpha-mannosidase activity, who were examined ophthalmologically, had similar lenticular opacities. Corneal opacities were absent. Chamber angle and striking ophthalmoscopic anomalies occurred in two young patients who had normal electroretinograms. Two patients had strabismus. Conjunctival biopsy specimens morphologically confirmed the lysosomal nature of this disorder.", "contents": "Ocular findings in mannosidosis. Three patients with typical features of mannosidosis and deficiency of alpha-mannosidase activity, who were examined ophthalmologically, had similar lenticular opacities. Corneal opacities were absent. Chamber angle and striking ophthalmoscopic anomalies occurred in two young patients who had normal electroretinograms. Two patients had strabismus. Conjunctival biopsy specimens morphologically confirmed the lysosomal nature of this disorder.", "PMID": 961797}
{"id": "pubmed23n0032_4661", "title": "Clinical and ultrastructural ocular histopathologic studies of adult-onset metachromatic leukodystrophy.", "content": "A twin brother and sister with adult-onset metachromatic leukodystrophy developed progressive central acuity loss and optic disk pallor. Both had normal electroretinograms and fluorescein angiography. Postmortem examination of the sister's eyes by histochemistry and electron microscopy revealed ganglion cell loss and optic atrophy. Cerebroside sulfate had accumulated in optic nerve glial cells. Optic atrophy was more advanced than in previously reported cases of infantile-onset metachromatic leukodystrophy. The pathologic process seemed to be retrograde optic nerve degeneration due to abnormal myelin metabolism.", "contents": "Clinical and ultrastructural ocular histopathologic studies of adult-onset metachromatic leukodystrophy. A twin brother and sister with adult-onset metachromatic leukodystrophy developed progressive central acuity loss and optic disk pallor. Both had normal electroretinograms and fluorescein angiography. Postmortem examination of the sister's eyes by histochemistry and electron microscopy revealed ganglion cell loss and optic atrophy. Cerebroside sulfate had accumulated in optic nerve glial cells. Optic atrophy was more advanced than in previously reported cases of infantile-onset metachromatic leukodystrophy. The pathologic process seemed to be retrograde optic nerve degeneration due to abnormal myelin metabolism.", "PMID": 961798}
{"id": "pubmed23n0032_4662", "title": "Adrenoleukodystrophy with disease of the eye and optic nerve.", "content": "Adrenoleukodystrophy is an X-chromosome-linked recessive disease characterized by primary atrophy of the adrenal glands with or without Addison's disease and low plasma cortisol levels, and a degeneration of white matter of the central nervous system with blindness. In suspected cases of adrenoleukodystrophy an impaired rise in plasma cortisol levels after adrenocorticotrophin stimulation may be diagnostic. With the electron microscope, pathognomonic intracytoplasmic lamellar inclusions have been seen in adrenal cortical cells, peripheral nerve Schwann's cells, testicular interstitial cells, and in macrophages of the brain. Adrenoleukodystrophy appears to be a genetically determined lipid storage disease with an error in membrane sterol metabolism. A 10-year-old boy with adrenoleukodystrophy had visual loss, a prominent early symptom. The ocular abnormality consisted of a disproportionate loss of nerve fibers from the macular region. No intracytoplasmic lamellar inclusions were identified in cells representing macrophages within the optic nerve. They contained myelin debris suggestive of end-stage disease.", "contents": "Adrenoleukodystrophy with disease of the eye and optic nerve. Adrenoleukodystrophy is an X-chromosome-linked recessive disease characterized by primary atrophy of the adrenal glands with or without Addison's disease and low plasma cortisol levels, and a degeneration of white matter of the central nervous system with blindness. In suspected cases of adrenoleukodystrophy an impaired rise in plasma cortisol levels after adrenocorticotrophin stimulation may be diagnostic. With the electron microscope, pathognomonic intracytoplasmic lamellar inclusions have been seen in adrenal cortical cells, peripheral nerve Schwann's cells, testicular interstitial cells, and in macrophages of the brain. Adrenoleukodystrophy appears to be a genetically determined lipid storage disease with an error in membrane sterol metabolism. A 10-year-old boy with adrenoleukodystrophy had visual loss, a prominent early symptom. The ocular abnormality consisted of a disproportionate loss of nerve fibers from the macular region. No intracytoplasmic lamellar inclusions were identified in cells representing macrophages within the optic nerve. They contained myelin debris suggestive of end-stage disease.", "PMID": 961799}
{"id": "pubmed23n0032_4663", "title": "Plasma cell infiltration of the conjunctiva associated with pancytopenia, dermatitis, and polyclonal gammopathy.", "content": "A 73-year-old man developed conjunctivitis, epiphora, pancytopenia, dermatitis, and a polyclonal gammopathy. Diffuse infiltration and thickening of the conjunctiva and lacrimal canaliculi by plasma cells was present. Histopathologic examination suggested a reactive, chronic inflammatory response rather than neoplasia. The patient's ocular manifestations appeared to be related to his pancytopenia, dermatitis, and polyclonal gammopathy.", "contents": "Plasma cell infiltration of the conjunctiva associated with pancytopenia, dermatitis, and polyclonal gammopathy. A 73-year-old man developed conjunctivitis, epiphora, pancytopenia, dermatitis, and a polyclonal gammopathy. Diffuse infiltration and thickening of the conjunctiva and lacrimal canaliculi by plasma cells was present. Histopathologic examination suggested a reactive, chronic inflammatory response rather than neoplasia. The patient's ocular manifestations appeared to be related to his pancytopenia, dermatitis, and polyclonal gammopathy.", "PMID": 961800}
{"id": "pubmed23n0032_4664", "title": "Increased intraocular pressure induced by corticosteroids.", "content": "Twelve patients who were highly responsive to topically administered betamethasone were subjected to various corticosteroid preparations (four times daily for four weeks). Each patient was tested in the same eye with fluorometholone, tetrahydrotriamcinolone, medrysone, and betamethasone and the potential to elvate intraocular pressure was determined. The response of intraocular pressure to all the corticosteroids was dose-related and a highly significant correlation was demonstrated between the concentration of betamethasone and the magnitude of intraocular pressure response. The order of potential of corticosteroids to elevate IOP was betamethasone 0.1%, betamethasone 0.05%, tetrahydrotriamcinolone 1.25%, betamethasone 0.02%, fluorometholone 0.1%, medrysone 1.0%, and betamethasone 0.01%. Tetrahydrotriamcinolone 0.25% and fluorometholone 0.05 and 0.01% failed to elevate intraocular pressure significantly.", "contents": "Increased intraocular pressure induced by corticosteroids. Twelve patients who were highly responsive to topically administered betamethasone were subjected to various corticosteroid preparations (four times daily for four weeks). Each patient was tested in the same eye with fluorometholone, tetrahydrotriamcinolone, medrysone, and betamethasone and the potential to elvate intraocular pressure was determined. The response of intraocular pressure to all the corticosteroids was dose-related and a highly significant correlation was demonstrated between the concentration of betamethasone and the magnitude of intraocular pressure response. The order of potential of corticosteroids to elevate IOP was betamethasone 0.1%, betamethasone 0.05%, tetrahydrotriamcinolone 1.25%, betamethasone 0.02%, fluorometholone 0.1%, medrysone 1.0%, and betamethasone 0.01%. Tetrahydrotriamcinolone 0.25% and fluorometholone 0.05 and 0.01% failed to elevate intraocular pressure significantly.", "PMID": 961801}
{"id": "pubmed23n0032_4665", "title": "Plasma cortisol suppression test used to predict the development of primary open-angle glaucoma.", "content": "Forty patients classified as high responders (GG) to dexamethasone testing (intraocular pressure greater than 31 mm Hg) without visual field loss were subjected to plasma cortisol suppression testing. After a five-year follow-up adequate data were available on 35 patients. Eighteen responded to 1.0 mg of dexamethasone-diphenylhydantoin suppression testing in a similar fashion to patients with primary open-angle glaucoma while 17 responded in a similar fashion to subjects classified as low (NN, intraocular pressure less than 20 mm Hg) and intermediate (NG, intraocular pressure 20 to 31 mm Hg) responders to dexamethasone testing. Eight of the 35 patients developed glaucomatous visual field loss during the follow-up period. These eight patients were not more sensitive to suppression of plasma cortisol than the 27 patients maintaining normal visual fields. Thus, plasma cortisol suppression testing failed to predict the development of primary open-angle glaucoma in GG responders.", "contents": "Plasma cortisol suppression test used to predict the development of primary open-angle glaucoma. Forty patients classified as high responders (GG) to dexamethasone testing (intraocular pressure greater than 31 mm Hg) without visual field loss were subjected to plasma cortisol suppression testing. After a five-year follow-up adequate data were available on 35 patients. Eighteen responded to 1.0 mg of dexamethasone-diphenylhydantoin suppression testing in a similar fashion to patients with primary open-angle glaucoma while 17 responded in a similar fashion to subjects classified as low (NN, intraocular pressure less than 20 mm Hg) and intermediate (NG, intraocular pressure 20 to 31 mm Hg) responders to dexamethasone testing. Eight of the 35 patients developed glaucomatous visual field loss during the follow-up period. These eight patients were not more sensitive to suppression of plasma cortisol than the 27 patients maintaining normal visual fields. Thus, plasma cortisol suppression testing failed to predict the development of primary open-angle glaucoma in GG responders.", "PMID": 961802}
{"id": "pubmed23n0032_4666", "title": "Congenital homonymous hemianopia.", "content": "We reviewed eight cases of congenital homonymous hemianopia to emphasize the features that may help the clinician to diagnose a congenital disease. The patients were usually unaware of the visual field defect. The hemianopia was usually congruous when incomplete and the opticokinetic nystagmus had a normal pattern. Associated congenital ocular and neurologic abnormalities were common. Clinically recognizable atrophy or hypoplasia of the optic disk was found in all but one of the patients. There was often a history of neonatal or gestational difficulties followed by emotional, behavioral, and learning problems without mental retardation.", "contents": "Congenital homonymous hemianopia. We reviewed eight cases of congenital homonymous hemianopia to emphasize the features that may help the clinician to diagnose a congenital disease. The patients were usually unaware of the visual field defect. The hemianopia was usually congruous when incomplete and the opticokinetic nystagmus had a normal pattern. Associated congenital ocular and neurologic abnormalities were common. Clinically recognizable atrophy or hypoplasia of the optic disk was found in all but one of the patients. There was often a history of neonatal or gestational difficulties followed by emotional, behavioral, and learning problems without mental retardation.", "PMID": 961803}
{"id": "pubmed23n0032_4667", "title": "An accidental tarsorrhaphy caused by acrylic adhesive.", "content": "A 10-year old boy accidently splashed acrylic adhesive in his left eye. I separated the eyelids by cutting adherent eyelashes with the use of a biomicroscope for magnification illumination. Tests with rabbits demonstrated that the solvent nitromethane dissolved the adhesions but diluted the glue and led to adhesions between the tarsal portion of the eyelid and the cornea. I used acrylic adhesions successfully for a temporary relief of irritation in a patient with Bell's palsy.", "contents": "An accidental tarsorrhaphy caused by acrylic adhesive. A 10-year old boy accidently splashed acrylic adhesive in his left eye. I separated the eyelids by cutting adherent eyelashes with the use of a biomicroscope for magnification illumination. Tests with rabbits demonstrated that the solvent nitromethane dissolved the adhesions but diluted the glue and led to adhesions between the tarsal portion of the eyelid and the cornea. I used acrylic adhesions successfully for a temporary relief of irritation in a patient with Bell's palsy.", "PMID": 961804}
{"id": "pubmed23n0032_4668", "title": "Marcus gunn (jaw-winking) syndrome.", "content": "Association of Marcus Gunn (jaw-winking) syndrome with ipsilateral familial cleft lip occurred in a 17-year-old white boy. He had anisometropia and esotropia with amblyopia of the involved eye. Jaw-winking and blepharoptosis improved to a cosmetically acceptable extent as the child grew older.", "contents": "Marcus gunn (jaw-winking) syndrome. Association of Marcus Gunn (jaw-winking) syndrome with ipsilateral familial cleft lip occurred in a 17-year-old white boy. He had anisometropia and esotropia with amblyopia of the involved eye. Jaw-winking and blepharoptosis improved to a cosmetically acceptable extent as the child grew older.", "PMID": 961806}
{"id": "pubmed23n0032_4669", "title": "Performance in occupational therapy as a predictor of successful prevocational training.", "content": "Scores on three subtests of the Minnesota Follow-up Scale (MFS) were related to client outcomes in a prevocational training program in a study of 26 clients who were referred from an adult psychiatric day treatment unit. The results of the study agreed with a prediction that successful performance in occupational therapy in the day treatment unit, as measured by subtest 1, would be significantly related to successful client outcomes. the results regarding predictions for two other subtests were less conclusive because clients received high scores. Age and sex distributions showed that the younger male clients were most likely to be referred for prevocational training. The staff of the prevocational training program accepted the results of the study and also agreed that performance in occupational therapy (in the day treatment unit) should receive greater recognition by staff members as an important indicator of work potential when evaluating clients for referral to a vocational training program. It is planned to continue the use of the MFS in the day treatment unit as an aid for evaluating clients for referral purposes. Scores will continue to be related to client outcomes in the prevocational training program. Further study is also planned concerning the relationship of age and sex and sources to which clients are discharged or referred from the day treatment unit.", "contents": "Performance in occupational therapy as a predictor of successful prevocational training. Scores on three subtests of the Minnesota Follow-up Scale (MFS) were related to client outcomes in a prevocational training program in a study of 26 clients who were referred from an adult psychiatric day treatment unit. The results of the study agreed with a prediction that successful performance in occupational therapy in the day treatment unit, as measured by subtest 1, would be significantly related to successful client outcomes. the results regarding predictions for two other subtests were less conclusive because clients received high scores. Age and sex distributions showed that the younger male clients were most likely to be referred for prevocational training. The staff of the prevocational training program accepted the results of the study and also agreed that performance in occupational therapy (in the day treatment unit) should receive greater recognition by staff members as an important indicator of work potential when evaluating clients for referral to a vocational training program. It is planned to continue the use of the MFS in the day treatment unit as an aid for evaluating clients for referral purposes. Scores will continue to be related to client outcomes in the prevocational training program. Further study is also planned concerning the relationship of age and sex and sources to which clients are discharged or referred from the day treatment unit.", "PMID": 961809}
{"id": "pubmed23n0032_4670", "title": "Behavior modification: a perspective.", "content": "Behavior modification is an approach for changing behavior based on a preliminary study of the individual. Behavior change is attempted once the individual's learning characteristics and the identification of the optimal learning conditions for the learner have been analyzed. The ability to analyze systematically and to develop behavior-environment relationships also brings a responsibility to the user to understand and master the principles and procedures of behavior modification. This article basically ignores the issue of selecting broad treatment goals and the authority of clinicians or teachers to work actively toward achieving these goals. The principal focus is to provide a perspective on the evaluation and use of behavior modification programs based on their conceptual and procedural foundations. Criticisms and the misuse of behavior modification programs are included as well as a set of guidelines for evaluating and developing effective ones.", "contents": "Behavior modification: a perspective. Behavior modification is an approach for changing behavior based on a preliminary study of the individual. Behavior change is attempted once the individual's learning characteristics and the identification of the optimal learning conditions for the learner have been analyzed. The ability to analyze systematically and to develop behavior-environment relationships also brings a responsibility to the user to understand and master the principles and procedures of behavior modification. This article basically ignores the issue of selecting broad treatment goals and the authority of clinicians or teachers to work actively toward achieving these goals. The principal focus is to provide a perspective on the evaluation and use of behavior modification programs based on their conceptual and procedural foundations. Criticisms and the misuse of behavior modification programs are included as well as a set of guidelines for evaluating and developing effective ones.", "PMID": 961811}
{"id": "pubmed23n0032_4671", "title": "Relationships between perceptual responses and accident patterns in children: a pilot study.", "content": "Many studies have been done to define factors related to accident tendencies in children, but few related these tendencies to developmental lags in visual perception. This pilot study explored the possibility that such a relationship exists. Twelve Caucasian females from similar socioeconomic backgrounds and age ranges were administered the Frostig Developmental Test of Visual Perception. Performance on the perceptual test was compared to accident frequency levels for each child to determine whether or not a correlation between visual perceptual performance and accident frequency existed. Although statistically significant results were not obtained, the need for more definite research related to spatial relations, position in space, and figure ground perception was demonstrated.", "contents": "Relationships between perceptual responses and accident patterns in children: a pilot study. Many studies have been done to define factors related to accident tendencies in children, but few related these tendencies to developmental lags in visual perception. This pilot study explored the possibility that such a relationship exists. Twelve Caucasian females from similar socioeconomic backgrounds and age ranges were administered the Frostig Developmental Test of Visual Perception. Performance on the perceptual test was compared to accident frequency levels for each child to determine whether or not a correlation between visual perceptual performance and accident frequency existed. Although statistically significant results were not obtained, the need for more definite research related to spatial relations, position in space, and figure ground perception was demonstrated.", "PMID": 961812}
{"id": "pubmed23n0032_4672", "title": "Additivity of effects within sectors of the sensitization zone of the Westheimer function.", "content": "In this paper, the fourth in a new series, we have examined the properties associated with illuminated sectors of the annular \"sensitization\" zone of the psychophysical Westheimer function. The results for one-, two-, and four-sector annular background fields indicate simple additivity of effects within the sensitization region when the flashing test field was set substantially above its increment threshold. Under such conditions effects of pattern orientation and borders within the sensitization zone were found to be negligible. The present findings provide a basis for relating the newly developed \"transient-like\" psychophysical test (which uses a windmill-type stimulus) to the Westheimer function. These data complement earlier clinical analyses of responses by patients whose Westheimer functions were studied near visual field lesions having sharp borders. The latter study is important in localizing this response function in the retina.", "contents": "Additivity of effects within sectors of the sensitization zone of the Westheimer function. In this paper, the fourth in a new series, we have examined the properties associated with illuminated sectors of the annular \"sensitization\" zone of the psychophysical Westheimer function. The results for one-, two-, and four-sector annular background fields indicate simple additivity of effects within the sensitization region when the flashing test field was set substantially above its increment threshold. Under such conditions effects of pattern orientation and borders within the sensitization zone were found to be negligible. The present findings provide a basis for relating the newly developed \"transient-like\" psychophysical test (which uses a windmill-type stimulus) to the Westheimer function. These data complement earlier clinical analyses of responses by patients whose Westheimer functions were studied near visual field lesions having sharp borders. The latter study is important in localizing this response function in the retina.", "PMID": 961817}
{"id": "pubmed23n0032_4673", "title": "Diffraction theory and visual hyperacuity.", "content": "The implications of the diffraction theory of light are examined as they relate to visual acuity and hyperacuity. Two incoherent point sources of light give rise to a double-peaked light distribution whose trough is 26% below the adjoining peaks when the sources are separated by Rayleigh limit of resolution, but the dip has vanished when the separation is 80% of the Rayleigh limit. Diffraction theory, however, places no restriction on the precision with which any single source can be localized. No paradox is therefore involved in having a point-spread function with half-width one minute of arc and, at the same time, a localization threshold of a few seconds of arc, although the need for a sophisticated processing mechanism to achieve such low hyperacuity thresholds is emphasized.", "contents": "Diffraction theory and visual hyperacuity. The implications of the diffraction theory of light are examined as they relate to visual acuity and hyperacuity. Two incoherent point sources of light give rise to a double-peaked light distribution whose trough is 26% below the adjoining peaks when the sources are separated by Rayleigh limit of resolution, but the dip has vanished when the separation is 80% of the Rayleigh limit. Diffraction theory, however, places no restriction on the precision with which any single source can be localized. No paradox is therefore involved in having a point-spread function with half-width one minute of arc and, at the same time, a localization threshold of a few seconds of arc, although the need for a sophisticated processing mechanism to achieve such low hyperacuity thresholds is emphasized.", "PMID": 961819}
{"id": "pubmed23n0032_4674", "title": "Ultrastructural evaluation of the interaction of glucocorticoids and vitamin D on bone cells in thyroparathyroidectomized rats.", "content": "To determine the direct effects of cortisol on bone, rats were thyroparathyroidectomized (T(X)PT(X)), fed a low-calcium diet, and given high (50 mg/kg) or low (8 mg/kg) pharmacologic levels of cortisol with or without excess vitamin D3 (15,000 IU). Rats given vitamin D had osteoblasts and osteocytes interpreted ultrastructurally to be actively engaged in matrix synthesis, mineralization of matrix, and in calcium mobilization. Osteoclasts were numerous on metaphyseal trabeculae and in vascular channels of cortical bone. In T(X)PT(X) rats not given vitamin D, osteoblasts and osteocytes were interpreted to have reduced metabolic activity with minimal evidence of participation in bone formation or resorption. Cortisol at both dose levels failed to alter the electron microscopic appearance of osteoblasts, osteocytes, and osteoclasts with or without vitamin D. Bone turnover indicated by urinary hydroxyproline excretion was unaffected by cortisol treatment. These findings suggest that glucocorticoids have little direct action on bone cells and that their effects on calcium metabolism are probably mediated by an interference in intestinal calcium transport and by secondary hyperparathyroidism.", "contents": "Ultrastructural evaluation of the interaction of glucocorticoids and vitamin D on bone cells in thyroparathyroidectomized rats. To determine the direct effects of cortisol on bone, rats were thyroparathyroidectomized (T(X)PT(X)), fed a low-calcium diet, and given high (50 mg/kg) or low (8 mg/kg) pharmacologic levels of cortisol with or without excess vitamin D3 (15,000 IU). Rats given vitamin D had osteoblasts and osteocytes interpreted ultrastructurally to be actively engaged in matrix synthesis, mineralization of matrix, and in calcium mobilization. Osteoclasts were numerous on metaphyseal trabeculae and in vascular channels of cortical bone. In T(X)PT(X) rats not given vitamin D, osteoblasts and osteocytes were interpreted to have reduced metabolic activity with minimal evidence of participation in bone formation or resorption. Cortisol at both dose levels failed to alter the electron microscopic appearance of osteoblasts, osteocytes, and osteoclasts with or without vitamin D. Bone turnover indicated by urinary hydroxyproline excretion was unaffected by cortisol treatment. These findings suggest that glucocorticoids have little direct action on bone cells and that their effects on calcium metabolism are probably mediated by an interference in intestinal calcium transport and by secondary hyperparathyroidism.", "PMID": 961822}
{"id": "pubmed23n0032_4675", "title": "Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni.", "content": "Exposure of the mouse skin to Schistosoma mansoni cercariae gives rise to acute, exudative inflammation in both normal and immune mice, but the immune response is anamnestically accelerated and is oesinophil-enriched, thereby enhancing opportunities for tegumental contact of schistosomula with host leukocytes, particularly with eosinophils. Many of the inflammatory changes occurring within the first 48 hours after exposure are due to cercarial products, e.g., \"penetration tracts,\" but some remain demonstrable when schistosomula metamorphosed in vitro are injected intradermally and are therefore directed against the schistosomula themselves, such as the leukocyte \"streaming patterns\" seen in their pathways. In contrast to earlier observations in primates, cellular responses to schistosomula in the mouse lung 4 days after penetration are minimal in either normal or immune mice. Thus, immune cellular responses to schistosomula in mice are limited to an early time period after cercarial penetration and are morphologically suggestive of an antibody-mediated response rather than of delayed hypersensitivity. Our observations complement earlier evidence suggesting that antibody-mediated host leukocyte contact with schistosomula initiates the killing of challenge parasites in immune mice, with the eosinophil probably playing a crucial role.", "contents": "Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. Exposure of the mouse skin to Schistosoma mansoni cercariae gives rise to acute, exudative inflammation in both normal and immune mice, but the immune response is anamnestically accelerated and is oesinophil-enriched, thereby enhancing opportunities for tegumental contact of schistosomula with host leukocytes, particularly with eosinophils. Many of the inflammatory changes occurring within the first 48 hours after exposure are due to cercarial products, e.g., \"penetration tracts,\" but some remain demonstrable when schistosomula metamorphosed in vitro are injected intradermally and are therefore directed against the schistosomula themselves, such as the leukocyte \"streaming patterns\" seen in their pathways. In contrast to earlier observations in primates, cellular responses to schistosomula in the mouse lung 4 days after penetration are minimal in either normal or immune mice. Thus, immune cellular responses to schistosomula in mice are limited to an early time period after cercarial penetration and are morphologically suggestive of an antibody-mediated response rather than of delayed hypersensitivity. Our observations complement earlier evidence suggesting that antibody-mediated host leukocyte contact with schistosomula initiates the killing of challenge parasites in immune mice, with the eosinophil probably playing a crucial role.", "PMID": 961823}
{"id": "pubmed23n0032_4676", "title": "A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro.", "content": "Whole blood serum (HBS) stimulates the proliferation of fibroblasts in vitro, while platelet-poor plasma serum (PPPS) does not. Fibroblasts grown in the presence of PPPS are truly quiescent in that they are not deprived of nutrients in the culture medium and less than 3% of the cells synthesize DNA and divide. In vivo experiments have suggested that macrophages are necessary for stimulation of fibroplasia during wound repair. We have utilized the difference in growth-promoting activity between HBS and PPPS to study the ability of macrophages to produce growth-promoting activity in cell culture. Guinea pig peritoneal macrophages cultured in vitro in medium containing PPPS release into the medium, either directly or indirectly, a factor (or factors) that stimulates the proliferation of guinea pig wound fibroblasts. This macrophage-dependent, fibroblast-stimulating activity (MFSA) is nondialyzable, heat stable (56 C for 30 minutes), and requires culture in vitro for demonstration of activity. The relationship between MFSA and other growth factor(s) has not yet been determined. In contrast to the macrophage, lymphocytes prepared from mesenteric lymph nodes produced no figroblast-stimulating activity.", "contents": "A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro. Whole blood serum (HBS) stimulates the proliferation of fibroblasts in vitro, while platelet-poor plasma serum (PPPS) does not. Fibroblasts grown in the presence of PPPS are truly quiescent in that they are not deprived of nutrients in the culture medium and less than 3% of the cells synthesize DNA and divide. In vivo experiments have suggested that macrophages are necessary for stimulation of fibroplasia during wound repair. We have utilized the difference in growth-promoting activity between HBS and PPPS to study the ability of macrophages to produce growth-promoting activity in cell culture. Guinea pig peritoneal macrophages cultured in vitro in medium containing PPPS release into the medium, either directly or indirectly, a factor (or factors) that stimulates the proliferation of guinea pig wound fibroblasts. This macrophage-dependent, fibroblast-stimulating activity (MFSA) is nondialyzable, heat stable (56 C for 30 minutes), and requires culture in vitro for demonstration of activity. The relationship between MFSA and other growth factor(s) has not yet been determined. In contrast to the macrophage, lymphocytes prepared from mesenteric lymph nodes produced no figroblast-stimulating activity.", "PMID": 961824}
{"id": "pubmed23n0032_4677", "title": "Effects of lipemia and trauma on experimental fat embolism.", "content": "According to some investigators, trauma is thought to bring about physical changes which produce a coalescence of normally dispersed blood lipids into fat droplets and pulmonary fat embolisms. Lipemia is thought to increase the extent of this embolization. If this theory is correct, intravenously infused labeled oil which is retained in the lungs should be mixed with and diluted by the fat emboli originating from the blood. Radioactive olive oil of known specific activity was slowly administered to a) control rats, b) rats made lipemic by dietary measures, c) severely traumatized rats, and d) traumatized lipemic rats. There was no difference in the specific activity of the oil recovered from the lungs of rats of any group, indicating that no recognizable amount of blood lipids had been added to the experimentally produced emboli.", "contents": "Effects of lipemia and trauma on experimental fat embolism. According to some investigators, trauma is thought to bring about physical changes which produce a coalescence of normally dispersed blood lipids into fat droplets and pulmonary fat embolisms. Lipemia is thought to increase the extent of this embolization. If this theory is correct, intravenously infused labeled oil which is retained in the lungs should be mixed with and diluted by the fat emboli originating from the blood. Radioactive olive oil of known specific activity was slowly administered to a) control rats, b) rats made lipemic by dietary measures, c) severely traumatized rats, and d) traumatized lipemic rats. There was no difference in the specific activity of the oil recovered from the lungs of rats of any group, indicating that no recognizable amount of blood lipids had been added to the experimentally produced emboli.", "PMID": 961825}
{"id": "pubmed23n0032_4678", "title": "Acute lung injury induced by cadmium aerosol. I. Evolution of alveolar cell damage.", "content": "Following exposure to an aerosol of 0.1% (.005 M) cadmium chloride, rat lungs were examined at 6 hours and 1, 2, 3, 4, 7, and 10 days. By light microscopy, damage was multifocal and centered about respiratory bronchioles. Ultrastructurally, there was Type 1 cell edema with frequent loss of surface plasma membranes during the first 24 hours. After 2 days, the number of Type 2 cells had markedly increased, and by 3 days the damaged alveoli were lined by plump cuboidal cells closely resembling Type 2 cells. By 4 days, these cells were flatter, the change being more marked by 7 days; by 10 days, the cells had regained the appearance of Type 1 cells through loss of osmiophilic bodies and superficial microvilli. We conclude that CdCl2 damages Type 1 cells, which are then replaced by proliferation of Type 2 cells. These cells lose their osmiophilic bodies and flatten out to replace the lost Type 1 cells, the process being almost complete by 10 days after the injury. This pattern resembles the injury caused by NO2, O3, and O2.", "contents": "Acute lung injury induced by cadmium aerosol. I. Evolution of alveolar cell damage. Following exposure to an aerosol of 0.1% (.005 M) cadmium chloride, rat lungs were examined at 6 hours and 1, 2, 3, 4, 7, and 10 days. By light microscopy, damage was multifocal and centered about respiratory bronchioles. Ultrastructurally, there was Type 1 cell edema with frequent loss of surface plasma membranes during the first 24 hours. After 2 days, the number of Type 2 cells had markedly increased, and by 3 days the damaged alveoli were lined by plump cuboidal cells closely resembling Type 2 cells. By 4 days, these cells were flatter, the change being more marked by 7 days; by 10 days, the cells had regained the appearance of Type 1 cells through loss of osmiophilic bodies and superficial microvilli. We conclude that CdCl2 damages Type 1 cells, which are then replaced by proliferation of Type 2 cells. These cells lose their osmiophilic bodies and flatten out to replace the lost Type 1 cells, the process being almost complete by 10 days after the injury. This pattern resembles the injury caused by NO2, O3, and O2.", "PMID": 961827}
{"id": "pubmed23n0032_4679", "title": "The adrenal cortex in spontaneously hypertensive rats. A quantitative ultrastructural study.", "content": "The adrenal cortex of spontaneously hypertensive rats (SHR) has been examined by quantitative morphologic techniques for electron microscopy. The volume and surface area of smooth endoplasmic reticulum and the volume of Golgi apparatuses in zona glomerulosa cells of SHR was significantly greater than those of Wistar-Kyoto strain (W/KY) normotensive controls; the volume of lipid droplets and nucleus was significantly less in SHR than in W/KY animals. A stimulation of the zona glomerulosa in SHR may well be attributable to the elevation in systolic blood pressure. A distinct lipid-free subglomerulosa was observed in the adrenal gland of W/KY rats; the cell volume was similar to that of the zona glomerulosa although the cells showed a significantly greater volume of mitochondria and surface area of mitochondrial membranes and greater volume of smooth endoplasmic reticulum and lysosomes. In the zona fasciculata, cell volume, volumes and surface area of mitochondria and smooth endoplasmic reticulum, and volume of lipid droplets were significantly lower in SHR than in W/KY rats. The volume of the Golgi apparatus was greater in SHR than in W/KY rats. Glycogen particles were observed in focal areas of some zona fasciculata cells. The adrenal cortex of another strain of normotensive Wistar rat (W/CFN) was compared with that of the W/KY and SHR. Although the relative adrenal weights of SHR and W/KY animals were identical, the weight of that in W/CFN was significantly smaller. The volume of the zona glomerulosa of SHR was significantly greater than that of W/KY although the volume of the zona glomerulosa in W/CFN was significantly greater than the other two groups. The volume of nucleus and lipid droplets of zona glomerulosa in W/KY was significantly greater than that in the S/CFN; the volume of the cell, mitochondria, smooth endoplasmic reticulum, lipid droplets, and lysosomes, and the surface area of smooth endoplasmic reticulum and mitochondrial membranes of W/KY animals was significantly greater than those of W/CFN animals. It is concluded that the W/CFN rat is not an appropriate control for spontaneously hypertensive rats.", "contents": "The adrenal cortex in spontaneously hypertensive rats. A quantitative ultrastructural study. The adrenal cortex of spontaneously hypertensive rats (SHR) has been examined by quantitative morphologic techniques for electron microscopy. The volume and surface area of smooth endoplasmic reticulum and the volume of Golgi apparatuses in zona glomerulosa cells of SHR was significantly greater than those of Wistar-Kyoto strain (W/KY) normotensive controls; the volume of lipid droplets and nucleus was significantly less in SHR than in W/KY animals. A stimulation of the zona glomerulosa in SHR may well be attributable to the elevation in systolic blood pressure. A distinct lipid-free subglomerulosa was observed in the adrenal gland of W/KY rats; the cell volume was similar to that of the zona glomerulosa although the cells showed a significantly greater volume of mitochondria and surface area of mitochondrial membranes and greater volume of smooth endoplasmic reticulum and lysosomes. In the zona fasciculata, cell volume, volumes and surface area of mitochondria and smooth endoplasmic reticulum, and volume of lipid droplets were significantly lower in SHR than in W/KY rats. The volume of the Golgi apparatus was greater in SHR than in W/KY rats. Glycogen particles were observed in focal areas of some zona fasciculata cells. The adrenal cortex of another strain of normotensive Wistar rat (W/CFN) was compared with that of the W/KY and SHR. Although the relative adrenal weights of SHR and W/KY animals were identical, the weight of that in W/CFN was significantly smaller. The volume of the zona glomerulosa of SHR was significantly greater than that of W/KY although the volume of the zona glomerulosa in W/CFN was significantly greater than the other two groups. The volume of nucleus and lipid droplets of zona glomerulosa in W/KY was significantly greater than that in the S/CFN; the volume of the cell, mitochondria, smooth endoplasmic reticulum, lipid droplets, and lysosomes, and the surface area of smooth endoplasmic reticulum and mitochondrial membranes of W/KY animals was significantly greater than those of W/CFN animals. It is concluded that the W/CFN rat is not an appropriate control for spontaneously hypertensive rats.", "PMID": 961826}
{"id": "pubmed23n0032_4680", "title": "Voluntary inhibition of the myotatic reflex and premotor response to joint angle displacement.", "content": "EMGs from the deltoid muscle of the nonpreferred arm were obtained from 18 subjects (9 males, 9 females). The EMGs resulted from a stretch reflex (reflex latency) from the fall of the relaxed arm from an electromagnet and from the resultant EMGs of a voluntary response to stop the preceived falling arm (premotor response). Ten normal trials were followed by 10 inhibition trials. By conscious relaxation of the arm and inhibition of the deltoid muscle, all subjects on most trials were able to completely eliminate the voluntary premotor responses. Elimination of the myotatic reflex proved much more difficult with two subjects completely inhibiting it on all trials and ten subjects on at least one trial; however when the reflex was not inhibited, the inhibited condition resulted in significantly reduced reflex duration and an increased reflex latency. No sex difference was found in the ability to inhibit the EMG responses.", "contents": "Voluntary inhibition of the myotatic reflex and premotor response to joint angle displacement. EMGs from the deltoid muscle of the nonpreferred arm were obtained from 18 subjects (9 males, 9 females). The EMGs resulted from a stretch reflex (reflex latency) from the fall of the relaxed arm from an electromagnet and from the resultant EMGs of a voluntary response to stop the preceived falling arm (premotor response). Ten normal trials were followed by 10 inhibition trials. By conscious relaxation of the arm and inhibition of the deltoid muscle, all subjects on most trials were able to completely eliminate the voluntary premotor responses. Elimination of the myotatic reflex proved much more difficult with two subjects completely inhibiting it on all trials and ten subjects on at least one trial; however when the reflex was not inhibited, the inhibited condition resulted in significantly reduced reflex duration and an increased reflex latency. No sex difference was found in the ability to inhibit the EMG responses.", "PMID": 961830}
{"id": "pubmed23n0032_4681", "title": "Effects of a specific ecutaneous cold stimulus on single motor unit activity of medial gastrocnemium muscle in man.", "content": "The effect of a specific skin cooling stimulus on single motor unit (SMU) activity in the underlying medial gastrocnemius muscle on forty normal subjects was examined by computer analysis of the real time occurrence of each unit during a 12 minute experiment. Subjects (N, 17) discharging SMUs at a comfortable resting frequency (5.2 +/- 0.9 Hz) showed a tendency toward inhibitory responses during the first minute of cooling. Most subjects (13 of 18) who discharged SMUs at a frequency of 0.5 Hz underwent a statistically significant increase in SMU activity during the first minute and then a significant reduction in activity during the next minute of cooling. The general findings suggest that motor outflow can be altered in muscle underlying a specific thermal stimulus and that the central excitatory state of the nervous system plays a large role in determining how cutaneous cooling information will be processed to affect motor changes.", "contents": "Effects of a specific ecutaneous cold stimulus on single motor unit activity of medial gastrocnemium muscle in man. The effect of a specific skin cooling stimulus on single motor unit (SMU) activity in the underlying medial gastrocnemius muscle on forty normal subjects was examined by computer analysis of the real time occurrence of each unit during a 12 minute experiment. Subjects (N, 17) discharging SMUs at a comfortable resting frequency (5.2 +/- 0.9 Hz) showed a tendency toward inhibitory responses during the first minute of cooling. Most subjects (13 of 18) who discharged SMUs at a frequency of 0.5 Hz underwent a statistically significant increase in SMU activity during the first minute and then a significant reduction in activity during the next minute of cooling. The general findings suggest that motor outflow can be altered in muscle underlying a specific thermal stimulus and that the central excitatory state of the nervous system plays a large role in determining how cutaneous cooling information will be processed to affect motor changes.", "PMID": 961831}
{"id": "pubmed23n0032_4682", "title": "Effect of diazepam on muscle contraction in spasticity.", "content": "The direct effect of diazepam on skeletal muscle has been examined in 15 patients with neurological lesions resulting in spasticity. Diazepam 15-30 mg. IV reduced the amplitude of the compound action potential of the direct muscle response (M response) and the isometric twitch tension. It is postulated that diazepam may affect the contractile properties of muscle and, possibly, the electrical properties of the muscle membrane. These peripheral effects may contribute to the reported clinical benefits of the drug in patients with spasticity including those patients with complete spinal lesions.", "contents": "Effect of diazepam on muscle contraction in spasticity. The direct effect of diazepam on skeletal muscle has been examined in 15 patients with neurological lesions resulting in spasticity. Diazepam 15-30 mg. IV reduced the amplitude of the compound action potential of the direct muscle response (M response) and the isometric twitch tension. It is postulated that diazepam may affect the contractile properties of muscle and, possibly, the electrical properties of the muscle membrane. These peripheral effects may contribute to the reported clinical benefits of the drug in patients with spasticity including those patients with complete spinal lesions.", "PMID": 961832}
{"id": "pubmed23n0032_4683", "title": "Lengthening contraction and interpretations of active state tension in the isometric twitch response of skeletal muscle.", "content": "The plateau of tetanic tension in a lengthening contraction of muscle at any given length is always greater than that in an isometric contraction at the same given length. To account for this excess of tension, it is suggested that the contractile machinery is \"locked\" in a strained condition that might make the muscle capable of bearing a greater tension in a lengthening contraction than it can develop in an isometric contraction. It is proposed that this strained condition also occurs in a lengthening contraction of the twitch response. If this proposal is valid it seems certain that the plateu of tension in the lengthening contraction of the twitch response cannot be equated with the plateau of tension in the isometric contraction of tetanus. The design of the original concept, that the plateau of active state tension in the lengthening contraction of the twitch is equal to the plateau of tension (Po) in the isometric contraction of tetanus, is based upon the assumption that the contractile component is neither lengthening nor shortening during both of these plateaus. This assumption is made without considering the possibility that the plateau in the lengthening contraction might be due to concurrent lengthening of series elastic elements and shortening of the contractile component. To account for the plateau of tension observed after quick lengthening in the early phase of twitch contraction indirect evidence is presented favoring the view that the quick lengthening during stretch is followed by slow lengthening and stress relaxation (decrease of tension) in series elastic elements and simultaneous shortening and increase of tension in the contractile component. When the original concept of active state tension in the twitch response is interpreted in the light of lengthening contraction, it is concluded that the labeled or implied Po for the plateau of the active state tension is unwarranted and confusing. It seems that the primary source of confusion is the assumption that the tension a muscle is capable of bearing in a lengthening contraction is equatable with the tension it can develop in an isometric contraction.", "contents": "Lengthening contraction and interpretations of active state tension in the isometric twitch response of skeletal muscle. The plateau of tetanic tension in a lengthening contraction of muscle at any given length is always greater than that in an isometric contraction at the same given length. To account for this excess of tension, it is suggested that the contractile machinery is \"locked\" in a strained condition that might make the muscle capable of bearing a greater tension in a lengthening contraction than it can develop in an isometric contraction. It is proposed that this strained condition also occurs in a lengthening contraction of the twitch response. If this proposal is valid it seems certain that the plateu of tension in the lengthening contraction of the twitch response cannot be equated with the plateau of tension in the isometric contraction of tetanus. The design of the original concept, that the plateau of active state tension in the lengthening contraction of the twitch is equal to the plateau of tension (Po) in the isometric contraction of tetanus, is based upon the assumption that the contractile component is neither lengthening nor shortening during both of these plateaus. This assumption is made without considering the possibility that the plateau in the lengthening contraction might be due to concurrent lengthening of series elastic elements and shortening of the contractile component. To account for the plateau of tension observed after quick lengthening in the early phase of twitch contraction indirect evidence is presented favoring the view that the quick lengthening during stretch is followed by slow lengthening and stress relaxation (decrease of tension) in series elastic elements and simultaneous shortening and increase of tension in the contractile component. When the original concept of active state tension in the twitch response is interpreted in the light of lengthening contraction, it is concluded that the labeled or implied Po for the plateau of the active state tension is unwarranted and confusing. It seems that the primary source of confusion is the assumption that the tension a muscle is capable of bearing in a lengthening contraction is equatable with the tension it can develop in an isometric contraction.", "PMID": 961833}
{"id": "pubmed23n0032_4684", "title": "African fossil tali: further multivariate morphometric studies.", "content": "Analysis of measurements from the tali of 21 individual fossil primates from Africa shows that the specimens fall into five clearly defined groups. Accordingly, these specimens have been included as groups along with extant species in a subsequent canonical analysis thus allowing the fossils to play their part in the determination of the canonical separations. The results of this procedure show that the five fossil groups lie in a part of the canonical space not occupied by any extant African primate. Their positions are between the envelope of Asiatic apes (Hylobates and Pongo) and the envelope of African forms near the edge which contains Pan and Papio. One fossil group is so similar to Hylobates that its talus may have functioned in locomotion in a parallel manner. Others lie near to Pongo in directions proceeding towards Pan and Papio and it is possible that this similarity may indicate remnants of morphological adaptation for climbing in these fossils. At the same time, however, individual specimens are closer to one or another of the extant groups and this considerable spread suggests that the locomotor adaptations as evidenced by talar morphology, of the primate fauna in Africa, may have been very different from those of the present day. This would not the inconsistent with the different habitats, floras and non-primate faunas that may have characterized the East African scene at these earlier times. Particular fossils from Olduvai and Kromdraai that are supposed to be australopithecine and therefore bipeds, are confirmed (Oxnard, '72; Lisowski et al., '74) as being totally different from man in their talar morphology and essentially rather similar to the majority of the other fossil tali examined.", "contents": "African fossil tali: further multivariate morphometric studies. Analysis of measurements from the tali of 21 individual fossil primates from Africa shows that the specimens fall into five clearly defined groups. Accordingly, these specimens have been included as groups along with extant species in a subsequent canonical analysis thus allowing the fossils to play their part in the determination of the canonical separations. The results of this procedure show that the five fossil groups lie in a part of the canonical space not occupied by any extant African primate. Their positions are between the envelope of Asiatic apes (Hylobates and Pongo) and the envelope of African forms near the edge which contains Pan and Papio. One fossil group is so similar to Hylobates that its talus may have functioned in locomotion in a parallel manner. Others lie near to Pongo in directions proceeding towards Pan and Papio and it is possible that this similarity may indicate remnants of morphological adaptation for climbing in these fossils. At the same time, however, individual specimens are closer to one or another of the extant groups and this considerable spread suggests that the locomotor adaptations as evidenced by talar morphology, of the primate fauna in Africa, may have been very different from those of the present day. This would not the inconsistent with the different habitats, floras and non-primate faunas that may have characterized the East African scene at these earlier times. Particular fossils from Olduvai and Kromdraai that are supposed to be australopithecine and therefore bipeds, are confirmed (Oxnard, '72; Lisowski et al., '74) as being totally different from man in their talar morphology and essentially rather similar to the majority of the other fossil tali examined.", "PMID": 961834}
{"id": "pubmed23n0032_4685", "title": "Genetic structure and heterozygosity in the kuru region, eastern highlands of New Guinea.", "content": "Genetic structure of 20 of the 37 linguistic groups in the Eastern Highlands of New Guinea including the kuru region is analyzed using information on blood groups and serum protein polymorphisms. The average individual is heterozygous at 28.6% of loci and the average number of alleles per locus is 1.234. Coefficients of kinship for linguistic groups range from 0.005 for the sweet potato cultivating North Fore to 0.075 for the isolated Pawaians whose dietary staple is sago and who depend more on hunting and gathering. As one selects linguistic groups with smaller and smaller population size and increasing isolation, one finds that kinship coefficients rise as much as tenfold, but there is no concomitant loss of heterozygosity or trend toward fixation of alleles. Genetic relationships established by genetic distance trees and by principal components analysis are comparable and are consistent with other anthropological observations.", "contents": "Genetic structure and heterozygosity in the kuru region, eastern highlands of New Guinea. Genetic structure of 20 of the 37 linguistic groups in the Eastern Highlands of New Guinea including the kuru region is analyzed using information on blood groups and serum protein polymorphisms. The average individual is heterozygous at 28.6% of loci and the average number of alleles per locus is 1.234. Coefficients of kinship for linguistic groups range from 0.005 for the sweet potato cultivating North Fore to 0.075 for the isolated Pawaians whose dietary staple is sago and who depend more on hunting and gathering. As one selects linguistic groups with smaller and smaller population size and increasing isolation, one finds that kinship coefficients rise as much as tenfold, but there is no concomitant loss of heterozygosity or trend toward fixation of alleles. Genetic relationships established by genetic distance trees and by principal components analysis are comparable and are consistent with other anthropological observations.", "PMID": 961835}
{"id": "pubmed23n0032_4686", "title": "Prenatal origins of carpal fusions.", "content": "As shown in 138 embryos and fetuses in the 40-285 mm crown-rump length range, carpal and carpal-metacarpal \"fusions\" arise from incomplete separation of the cartilaginous precursors rather than from failure of initiation, thus accounting for the \"fusions\" seen in postnatal radiographs and the grooves that are evident enough in adult fusions. Radiographs selected from over 20,000 apparently normal individuals provide postnatal counterparts for the prenatal examples shown in histological sections.", "contents": "Prenatal origins of carpal fusions. As shown in 138 embryos and fetuses in the 40-285 mm crown-rump length range, carpal and carpal-metacarpal \"fusions\" arise from incomplete separation of the cartilaginous precursors rather than from failure of initiation, thus accounting for the \"fusions\" seen in postnatal radiographs and the grooves that are evident enough in adult fusions. Radiographs selected from over 20,000 apparently normal individuals provide postnatal counterparts for the prenatal examples shown in histological sections.", "PMID": 961836}
{"id": "pubmed23n0032_4687", "title": "Paradoxical bilateral asymmetry in bone size and bone mass in the hand.", "content": "Among 227 chronic renal disease patients, micrometer caliper radiogrammetric measurements of the second metacarpal at midshaft showed the right metacarpal to be larger (with greater bone area) and with a greater cortical area than the left second metacarpal, both in 208 right-handed individuals and in 19 left-handed individuals. This direction of asymmetry was individually characteristic of the majority of individuals, whether right-handed, left-handed or ambidextrous.", "contents": "Paradoxical bilateral asymmetry in bone size and bone mass in the hand. Among 227 chronic renal disease patients, micrometer caliper radiogrammetric measurements of the second metacarpal at midshaft showed the right metacarpal to be larger (with greater bone area) and with a greater cortical area than the left second metacarpal, both in 208 right-handed individuals and in 19 left-handed individuals. This direction of asymmetry was individually characteristic of the majority of individuals, whether right-handed, left-handed or ambidextrous.", "PMID": 961837}
{"id": "pubmed23n0032_4688", "title": "A study of nine polymorphic systems in the population of the Po Delta.", "content": "The present work reports a study of nine genetic polymorphic systems in the area of the Po Delta where malaria was endemic since the XIV century. Our data confirm some characteristics of this population already reported by other authors such as the high prevalence of thalassemia, the low prevalence of the rh (d) gene and the presence of G-6-PD deficiency. Among the other systems studied, i.e., AP, PGM1 ADA and AK, only AP frequencies of Po Delta population are significantly different from those of other continental Italian populations, the PC allele showing the lowest frequency so far observed.", "contents": "A study of nine polymorphic systems in the population of the Po Delta. The present work reports a study of nine genetic polymorphic systems in the area of the Po Delta where malaria was endemic since the XIV century. Our data confirm some characteristics of this population already reported by other authors such as the high prevalence of thalassemia, the low prevalence of the rh (d) gene and the presence of G-6-PD deficiency. Among the other systems studied, i.e., AP, PGM1 ADA and AK, only AP frequencies of Po Delta population are significantly different from those of other continental Italian populations, the PC allele showing the lowest frequency so far observed.", "PMID": 961838}
{"id": "pubmed23n0032_4689", "title": "Differential contribution of stature phenotypes to assortative mating in parents of Philadelphia black and white school children.", "content": "Assortative mating for stature, weight, and the ponderal and Quetelet's indices is considered in a large sample of Philadelphia Blacks and Whites. The husband-wife correlation for stature is positive and significant in Whites (r = 0.34, d.f. = 382), but negligible in Blacks (r = 0.06, d.f. = 420). Correlations for weight and the body indices are positive and significant in both samples, those for body indices showing some dependency on the husband-wife correlations for stature and weight. When couples are grouped into statural mating combinations on the basis of short (S), medium (M) and tall (T), White spouses' statures show an approximately linear relationship to one another. The distribution of Black spouses' statures, however, is not completely independent, even though the husband-wife correlation is close to zero. There are elements of both positive and negative assortative mating among Blacks, resulting in an excess of certain mating types over that expected on the basis of chance. These mating types are usually those in which the husband is shorter than the wife, except at the heterogenous extremes of the bivariate array.", "contents": "Differential contribution of stature phenotypes to assortative mating in parents of Philadelphia black and white school children. Assortative mating for stature, weight, and the ponderal and Quetelet's indices is considered in a large sample of Philadelphia Blacks and Whites. The husband-wife correlation for stature is positive and significant in Whites (r = 0.34, d.f. = 382), but negligible in Blacks (r = 0.06, d.f. = 420). Correlations for weight and the body indices are positive and significant in both samples, those for body indices showing some dependency on the husband-wife correlations for stature and weight. When couples are grouped into statural mating combinations on the basis of short (S), medium (M) and tall (T), White spouses' statures show an approximately linear relationship to one another. The distribution of Black spouses' statures, however, is not completely independent, even though the husband-wife correlation is close to zero. There are elements of both positive and negative assortative mating among Blacks, resulting in an excess of certain mating types over that expected on the basis of chance. These mating types are usually those in which the husband is shorter than the wife, except at the heterogenous extremes of the bivariate array.", "PMID": 961839}
{"id": "pubmed23n0032_4690", "title": "Metering of fluid intake and determinants of ad libitum drinking in rats.", "content": "The contributions of homeostatis mechanisms to spontaneous drinking were assessed in a study of residual oral drinking under several conditions of exogenous water administration. Continuous and/or discontinuous infusiors were conducted for long periods with dry food present ad libitum. The routes of administration were intravenous (IV), intragastric (IG) through a nasopharyngeal catheter. All infusions  reduced from noninfused levels, but the magnitude of that reduction was a function of both route and temporal characteristics. Continuous infusions were more effective via the IG route than IV, and a residual intake of about 10 ml/24 h presisted at even the highest infusion rates. Discontinuous meal-paired IV infusions suppressed mid- and postmeal drinking, consistent with the  satisfaction of systemic needs induced by cry food intake. Meal-paired IG nasopharyngeal infusions competely suppressed drinking when the infusions exceeded  base-line intake by only about 20%. In marked contrast, corresponding IG infusions through a direct fistula catheter were relatively ineffective. In all cases the decreases in drinking were not of a nonspecific nature beacuse food intake was unchanged. The discussion considers the different levels of metering involved and the time-varying (derivative) nature of the infusion if was suggested that there is a nonhomeostatic contribution to normal drinking.", "contents": "Metering of fluid intake and determinants of ad libitum drinking in rats. The contributions of homeostatis mechanisms to spontaneous drinking were assessed in a study of residual oral drinking under several conditions of exogenous water administration. Continuous and/or discontinuous infusiors were conducted for long periods with dry food present ad libitum. The routes of administration were intravenous (IV), intragastric (IG) through a nasopharyngeal catheter. All infusions  reduced from noninfused levels, but the magnitude of that reduction was a function of both route and temporal characteristics. Continuous infusions were more effective via the IG route than IV, and a residual intake of about 10 ml/24 h presisted at even the highest infusion rates. Discontinuous meal-paired IV infusions suppressed mid- and postmeal drinking, consistent with the  satisfaction of systemic needs induced by cry food intake. Meal-paired IG nasopharyngeal infusions competely suppressed drinking when the infusions exceeded  base-line intake by only about 20%. In marked contrast, corresponding IG infusions through a direct fistula catheter were relatively ineffective. In all cases the decreases in drinking were not of a nonspecific nature beacuse food intake was unchanged. The discussion considers the different levels of metering involved and the time-varying (derivative) nature of the infusion if was suggested that there is a nonhomeostatic contribution to normal drinking.", "PMID": 961847}
{"id": "pubmed23n0032_4691", "title": "Isohemic blood volume expansion in normal and areflexive dogs.", "content": "The hemodynamic and renal responses to rapid intravascular volume expansion (VE) were studied in normal and areflexive dogs. \"Isohemic\" expansion was performed by infusing autologous blood (averaging 453 ml) thoroughly mixed with the circulating blood. In areflexive dogs cardiac output and arterial pressure doubled immediately after VE and fell back to control within 70 min; in normal dogs the circulatory response was less than one-third as great, but arterial pressure failed to return to control within 120 min. In the areflexive dogs, water, electrolyte, and total osmolar urinary output rose three- to fivefold after VE and declined thereafter, roughly following arterial pressure. In normal dogs the urine flow increased 40% immediately and rose further up to 70% in 60 min. No evidence for a natriuretic hormone was seen. It is concluded that mechanical factors are mainly responsible for the increased excretion of water and solutes after VE. Direct nervous reflexes to the kidney seemed to play a quantitatively minor role in the renal response.", "contents": "Isohemic blood volume expansion in normal and areflexive dogs. The hemodynamic and renal responses to rapid intravascular volume expansion (VE) were studied in normal and areflexive dogs. \"Isohemic\" expansion was performed by infusing autologous blood (averaging 453 ml) thoroughly mixed with the circulating blood. In areflexive dogs cardiac output and arterial pressure doubled immediately after VE and fell back to control within 70 min; in normal dogs the circulatory response was less than one-third as great, but arterial pressure failed to return to control within 120 min. In the areflexive dogs, water, electrolyte, and total osmolar urinary output rose three- to fivefold after VE and declined thereafter, roughly following arterial pressure. In normal dogs the urine flow increased 40% immediately and rose further up to 70% in 60 min. No evidence for a natriuretic hormone was seen. It is concluded that mechanical factors are mainly responsible for the increased excretion of water and solutes after VE. Direct nervous reflexes to the kidney seemed to play a quantitatively minor role in the renal response.", "PMID": 961848}
{"id": "pubmed23n0032_4692", "title": "Arachidonic acid in splanchnic artery occlusion shock.", "content": "Arachindonic acid infused into the mesenteric vascular bed of normal pentobarbital-anesthetized dogs at a concentrations of 150 mug/kg per min produced no significant changes in mean arterial blood pressure (MABP), portal vein pressure (PVP), screen filtration pressure (SFP), platelet count, circulating lysosomal enzyme or myocardial depressant factor (MCF) activities, nad only modestly increased superior mesenteric arter flow (SMAF)ans endogenous prostaglandin concentrations concentrations. It is concluded that arachidonic acid, at the infusion rate employed, dose not have any major effect on the circulatory status or on the lysosomal or platelet stability in normal dogs. In contrast, arachidonic acid administered to dogs in splanchnic artery occulusion (SAO) shock significantly exacerbated the decline in MABP seen after release of the occlusive clamps and also significantly reduced mesenteric blood flow. The hypothnsive of arachidonic acid appears to be partly due to the fatty acid itself and partly due to the metavolically formed prostaglandin endoct the platelet count or aggregability, lysosomal hydrolase activity, or MDF formation in the SAO shock dogs. These data suggest that increased endogenous prostaglandin concentrations in themselves are not a prime factor in the pathophysiology of circulatory shock, but that endogenous prostaglandin or related substances can significantly modulate the shock state.", "contents": "Arachidonic acid in splanchnic artery occlusion shock. Arachindonic acid infused into the mesenteric vascular bed of normal pentobarbital-anesthetized dogs at a concentrations of 150 mug/kg per min produced no significant changes in mean arterial blood pressure (MABP), portal vein pressure (PVP), screen filtration pressure (SFP), platelet count, circulating lysosomal enzyme or myocardial depressant factor (MCF) activities, nad only modestly increased superior mesenteric arter flow (SMAF)ans endogenous prostaglandin concentrations concentrations. It is concluded that arachidonic acid, at the infusion rate employed, dose not have any major effect on the circulatory status or on the lysosomal or platelet stability in normal dogs. In contrast, arachidonic acid administered to dogs in splanchnic artery occulusion (SAO) shock significantly exacerbated the decline in MABP seen after release of the occlusive clamps and also significantly reduced mesenteric blood flow. The hypothnsive of arachidonic acid appears to be partly due to the fatty acid itself and partly due to the metavolically formed prostaglandin endoct the platelet count or aggregability, lysosomal hydrolase activity, or MDF formation in the SAO shock dogs. These data suggest that increased endogenous prostaglandin concentrations in themselves are not a prime factor in the pathophysiology of circulatory shock, but that endogenous prostaglandin or related substances can significantly modulate the shock state.", "PMID": 961849}
{"id": "pubmed23n0032_4693", "title": "PCO(2) and rate of formation of cerebrospinal fluid in the monkey.", "content": "Changes in cerebrospinal fluid formation rate (VF) with hypocapnia were measured by the ventriculocisternal perfusion technique in 24 rhesus monkeys anesthetized with nitrous oxide. In addition cerebral blood flow (CBF) was measured by the hydrogen clearence methods, Vf in control animals declined at a mean rate of 2.3 mul/min each hour during the last 4.5 h of a 7-h perfusion although variables known to effect Vf remained stable. Three hours after perfusion began, Vf of normocapnic controls was 41.4 mul/min +/- 5.4; CBF, 59P ML/100 G PER MIN. When Pco(2) was reduced to half of control, Vf fell to 35.6 mul/min +/- 6.3 and CBF fell by 27%. When Pco(2) was doubled, Vf fell to 33.1 mul/min +/- 5.3 and CBF increased threefold. The difference in Vf id significant only for the hypercapnic group (p=0.01). When animals were used as their own controls, three were no significant differences in Vf with hypercapnia compared to normocapnia. These results indicated that in the monkey variations of Pco(2) within broad physiologic limits, which are sufficient to cause large changes in CBF, have little effect of Vf.", "contents": "PCO(2) and rate of formation of cerebrospinal fluid in the monkey. Changes in cerebrospinal fluid formation rate (VF) with hypocapnia were measured by the ventriculocisternal perfusion technique in 24 rhesus monkeys anesthetized with nitrous oxide. In addition cerebral blood flow (CBF) was measured by the hydrogen clearence methods, Vf in control animals declined at a mean rate of 2.3 mul/min each hour during the last 4.5 h of a 7-h perfusion although variables known to effect Vf remained stable. Three hours after perfusion began, Vf of normocapnic controls was 41.4 mul/min +/- 5.4; CBF, 59P ML/100 G PER MIN. When Pco(2) was reduced to half of control, Vf fell to 35.6 mul/min +/- 6.3 and CBF fell by 27%. When Pco(2) was doubled, Vf fell to 33.1 mul/min +/- 5.3 and CBF increased threefold. The difference in Vf id significant only for the hypercapnic group (p=0.01). When animals were used as their own controls, three were no significant differences in Vf with hypercapnia compared to normocapnia. These results indicated that in the monkey variations of Pco(2) within broad physiologic limits, which are sufficient to cause large changes in CBF, have little effect of Vf.", "PMID": 961850}
{"id": "pubmed23n0032_4694", "title": "Pineal multiunit activity in conscious quail: effects of light, blinding, ganglionectomy.", "content": "Multiunit activity (MUA) was recorded from thr pineal gland in unanesthetized male Japenese quail. The influence of light regimen on spontaneous and flash-evoked firing patterns of pineal cells was investigated. Shortening the day length from 18 to 6 h/24 h resulted in a marked increase in pineal MUA. Pineal firing rate was higher during the dark than during the light fraction of the photoperiod. Flashlight stimulation always led to a marked inhibition of the pineal MUA. Cutting the optic nerves bilaterally resulted in a marked increase in the level of MUA. None of the experimental conditions altered the elevated MUA in blinded birds. Thus, photoperiod and light stimulation appeared to be important for pineal activity; however, no direct photosended quail. Superior cervical ganglionectomy resulted in slightly increased spontaneous MUA but did not inhibit pineal responses to flashlight stimulations.", "contents": "Pineal multiunit activity in conscious quail: effects of light, blinding, ganglionectomy. Multiunit activity (MUA) was recorded from thr pineal gland in unanesthetized male Japenese quail. The influence of light regimen on spontaneous and flash-evoked firing patterns of pineal cells was investigated. Shortening the day length from 18 to 6 h/24 h resulted in a marked increase in pineal MUA. Pineal firing rate was higher during the dark than during the light fraction of the photoperiod. Flashlight stimulation always led to a marked inhibition of the pineal MUA. Cutting the optic nerves bilaterally resulted in a marked increase in the level of MUA. None of the experimental conditions altered the elevated MUA in blinded birds. Thus, photoperiod and light stimulation appeared to be important for pineal activity; however, no direct photosended quail. Superior cervical ganglionectomy resulted in slightly increased spontaneous MUA but did not inhibit pineal responses to flashlight stimulations.", "PMID": 961851}
{"id": "pubmed23n0032_4695", "title": "Effects of lactate and glutamine on palmitate metabolism in rat kindey cortex.", "content": "Rat renal cortical slices were incubated with [1-(14)C]palmitate bound to 2.5% albumin. The following effects were found: a)1 mM palmitate utilization or oxidation to CO(2) varied according to the concentration of lactate in the media, it increased at 0.8 and 3.2 mM, was unchanged at 8 mM, and decreased at 16 mM. Esterification was stimulated at 3.2 mM lactate. b) Addition of glutamine (0.1 mM) instead of lactate stimulated incomplete and complete oxidation of palmitate (1 mM), whereas high medium glutamine (10 mM) inhibited palmitate (1 mM) utilization, esterification, and oxidation to CO(2) but increased its incomplete oxidation. The low rate of exogenous palmitate oxidation observed in this study and the finding that exogenous palmitate oxidation is only partially inhibited at very high concentrations of exogenous lactate or glutamine are consistent with the view that these exogenous substrates contribute little to the oxidative metabolism of rat renal cortex in vitro, which probably depends on the supply of substrates endogenous to the tissue.", "contents": "Effects of lactate and glutamine on palmitate metabolism in rat kindey cortex. Rat renal cortical slices were incubated with [1-(14)C]palmitate bound to 2.5% albumin. The following effects were found: a)1 mM palmitate utilization or oxidation to CO(2) varied according to the concentration of lactate in the media, it increased at 0.8 and 3.2 mM, was unchanged at 8 mM, and decreased at 16 mM. Esterification was stimulated at 3.2 mM lactate. b) Addition of glutamine (0.1 mM) instead of lactate stimulated incomplete and complete oxidation of palmitate (1 mM), whereas high medium glutamine (10 mM) inhibited palmitate (1 mM) utilization, esterification, and oxidation to CO(2) but increased its incomplete oxidation. The low rate of exogenous palmitate oxidation observed in this study and the finding that exogenous palmitate oxidation is only partially inhibited at very high concentrations of exogenous lactate or glutamine are consistent with the view that these exogenous substrates contribute little to the oxidative metabolism of rat renal cortex in vitro, which probably depends on the supply of substrates endogenous to the tissue.", "PMID": 961852}
{"id": "pubmed23n0032_4696", "title": "Hyperosmolarity: effects on nerves and smooth muscle of cutaneous veins.", "content": "Helical strips from dogs' saphenous veins were mounted in an organ bath (Krebs-Ringer solution, 37 degrees C) for isometric tension recording. Additional strips were incubated with [7-3H]norepinephrine and mounted for superfusion and isometric tension recording. The perfusate was collected every 2 min for estimation of total radioactivity and for column chromatographic separation of [3H]norepinephrine and its metabolites. Increasing osmolarity by sucrose caused a slow contraction that was maximal at 500-550 mosM and was accompanied by a reduction in total 3H efflux. The contraction was unaffected by a Ca2+-free medium, alpha-adrenegic blockade, and beta-adrenergic stimulaton. It was depressed by cooling (29 degrees C) and by anoxia combined with a glucose-free medium. Contractions elicited by K+ and Ba2+ were augmented by hyperosmolarity, but those caused by sympathetic nerve stimulation, tyramine, and norepinephrine were depressed. The output of [3H]norepinephrine during nerve stimulation was reduced. Thus, the hyperosmolarity causes 1) contraction of vascular smooth muscle, 2) augmentation of the contractile response to K+ and Ba2+, 3) depression of the excitation caused by norepinephrine, and 4) inhibition of the neuronal release of norepinephrine.", "contents": "Hyperosmolarity: effects on nerves and smooth muscle of cutaneous veins. Helical strips from dogs' saphenous veins were mounted in an organ bath (Krebs-Ringer solution, 37 degrees C) for isometric tension recording. Additional strips were incubated with [7-3H]norepinephrine and mounted for superfusion and isometric tension recording. The perfusate was collected every 2 min for estimation of total radioactivity and for column chromatographic separation of [3H]norepinephrine and its metabolites. Increasing osmolarity by sucrose caused a slow contraction that was maximal at 500-550 mosM and was accompanied by a reduction in total 3H efflux. The contraction was unaffected by a Ca2+-free medium, alpha-adrenegic blockade, and beta-adrenergic stimulaton. It was depressed by cooling (29 degrees C) and by anoxia combined with a glucose-free medium. Contractions elicited by K+ and Ba2+ were augmented by hyperosmolarity, but those caused by sympathetic nerve stimulation, tyramine, and norepinephrine were depressed. The output of [3H]norepinephrine during nerve stimulation was reduced. Thus, the hyperosmolarity causes 1) contraction of vascular smooth muscle, 2) augmentation of the contractile response to K+ and Ba2+, 3) depression of the excitation caused by norepinephrine, and 4) inhibition of the neuronal release of norepinephrine.", "PMID": 961853}
{"id": "pubmed23n0032_4697", "title": "Thermoregulatory effects of monoamine potentiators and inhibitors in the rat.", "content": "Exogenously administered monoamines may elicit variable thermoregulatory responses dependent on dosage, species, site of administration, ambient temperature, etc. In an attempt to reconcile several inconsistencies, we have undertaken a series of studies related to monoaminergic control of temperature regulation. Thus, intraventricular administration of serotonin (2.64-26.4 mug) and norepinephrine (3.3-32.8 mug) in rats evoked acute (15-60 min) dose-dependent hypothermic responses (delta Tre = 2 degrees C) that were gradually superseded by significant, more persistent hyperthermia (delta Tre = 1 degreee C). Administration of chlorimipramine or imipramine (total dose 40 mug), even in monoamine-depleted animals, caused long-lasting hyperthermic responses, presumably by the prevention of reuptake of serotonin and norepinephrine at nerve terminals involved in thermoregulation. Pretreatment with the serotonin inhibitor cyproheptadine (4o mug) attenuated the hyperthermia achieved by central administration of chlorimipramine alone. We conclude that both monoamines can act as thermogenic agents under the conditions of these experiments.", "contents": "Thermoregulatory effects of monoamine potentiators and inhibitors in the rat. Exogenously administered monoamines may elicit variable thermoregulatory responses dependent on dosage, species, site of administration, ambient temperature, etc. In an attempt to reconcile several inconsistencies, we have undertaken a series of studies related to monoaminergic control of temperature regulation. Thus, intraventricular administration of serotonin (2.64-26.4 mug) and norepinephrine (3.3-32.8 mug) in rats evoked acute (15-60 min) dose-dependent hypothermic responses (delta Tre = 2 degrees C) that were gradually superseded by significant, more persistent hyperthermia (delta Tre = 1 degreee C). Administration of chlorimipramine or imipramine (total dose 40 mug), even in monoamine-depleted animals, caused long-lasting hyperthermic responses, presumably by the prevention of reuptake of serotonin and norepinephrine at nerve terminals involved in thermoregulation. Pretreatment with the serotonin inhibitor cyproheptadine (4o mug) attenuated the hyperthermia achieved by central administration of chlorimipramine alone. We conclude that both monoamines can act as thermogenic agents under the conditions of these experiments.", "PMID": 961854}
{"id": "pubmed23n0032_4698", "title": "Metabolic adaptations in brown adipose tissue of the hamster in extreme ambient temperatures.", "content": "Cold acclimation caused the following changes in the brown adipose tissue (BAT) of the hamster: the relative weight of the tissue increased, it color darkened, the multilocular structure predominated, and tissue protein content increased while fat content decreased. There was also an increase in the mitochondrial protein content. Heat acclimation had the opposite effects, i.e., the color became lighter, total and mitochondrial protein decreased, fat content increased, and tissue structure was mostly unilocular. Accordingly, cold acclimation was accompanied by increased tissue respiration in the presence of chi-glycerophosphate (chi-GP) and succinate, whereas heat acclimation reduced the respiratory activity of the tissue with these substrates. Isolated BAT mitochondria from cold-acclimated animals increased activities of chi-GP and NADH oxidase, whereas the activities of succinic and cytochrome oxidases and the amount of mitochondrial cytochromes were unchanged. The effects of heat acclimation were more pronounced: there was a decrease in the activities of chi-GP, succinic, NADH, and cytochrome oxidases, as well as in the cytochrome a and a3 content. When respiration of tissue slices on succinate was compared to the maximal potential respiration, as measured with mitochondria disrupted by freezing and thawing, it was found that the relative activity (slices vs. disrupted mitochondria) was highest in cold-acclimated animals and decreased progressively with increasing acclimation temperatures. It is suggested that the differences in the apparent activity of the mitochondria were due to changes in the conformation of the mitochondria as a result of acclimation.", "contents": "Metabolic adaptations in brown adipose tissue of the hamster in extreme ambient temperatures. Cold acclimation caused the following changes in the brown adipose tissue (BAT) of the hamster: the relative weight of the tissue increased, it color darkened, the multilocular structure predominated, and tissue protein content increased while fat content decreased. There was also an increase in the mitochondrial protein content. Heat acclimation had the opposite effects, i.e., the color became lighter, total and mitochondrial protein decreased, fat content increased, and tissue structure was mostly unilocular. Accordingly, cold acclimation was accompanied by increased tissue respiration in the presence of chi-glycerophosphate (chi-GP) and succinate, whereas heat acclimation reduced the respiratory activity of the tissue with these substrates. Isolated BAT mitochondria from cold-acclimated animals increased activities of chi-GP and NADH oxidase, whereas the activities of succinic and cytochrome oxidases and the amount of mitochondrial cytochromes were unchanged. The effects of heat acclimation were more pronounced: there was a decrease in the activities of chi-GP, succinic, NADH, and cytochrome oxidases, as well as in the cytochrome a and a3 content. When respiration of tissue slices on succinate was compared to the maximal potential respiration, as measured with mitochondria disrupted by freezing and thawing, it was found that the relative activity (slices vs. disrupted mitochondria) was highest in cold-acclimated animals and decreased progressively with increasing acclimation temperatures. It is suggested that the differences in the apparent activity of the mitochondria were due to changes in the conformation of the mitochondria as a result of acclimation.", "PMID": 961855}
{"id": "pubmed23n0032_4699", "title": "Influence of vasopressin and amiloride on shunt pathways of frog skin.", "content": "Studies were done to determine the influence of vasopressin and amiloride on the sodium backflux of nonedge-damaged frog skin. As before, the bidirectional fluxes of sodium appeared to be limited primarily to the active transport pathways of control skins. When the skins were treated with vasopressin, the sodium backflux was increased selectively (over that of sucrose) and more so than  was expected for the active transportpathway alone. In control skins, amiloride had no effect on the flux of the shunt pathway. However, with vasopressin-treated skins, amiloride at 10(-6) M decreased the sodium backflux of the shunt pathway to levels observed previously for the control skins. These observations are compatible with the idea that the shunt pathway may in part be cellular and in part extracellular. When the changes in open-circuit voltage and shunt resistance caused by vasopressin and amiloride (as determined from the I-V relationships) were examined, the data were also found to be compatible with the idea that vasopressin exerted an influence on the shunt pathway of the frog skin, and this effect of vasopressin could be reversed by amiloride.", "contents": "Influence of vasopressin and amiloride on shunt pathways of frog skin. Studies were done to determine the influence of vasopressin and amiloride on the sodium backflux of nonedge-damaged frog skin. As before, the bidirectional fluxes of sodium appeared to be limited primarily to the active transport pathways of control skins. When the skins were treated with vasopressin, the sodium backflux was increased selectively (over that of sucrose) and more so than  was expected for the active transportpathway alone. In control skins, amiloride had no effect on the flux of the shunt pathway. However, with vasopressin-treated skins, amiloride at 10(-6) M decreased the sodium backflux of the shunt pathway to levels observed previously for the control skins. These observations are compatible with the idea that the shunt pathway may in part be cellular and in part extracellular. When the changes in open-circuit voltage and shunt resistance caused by vasopressin and amiloride (as determined from the I-V relationships) were examined, the data were also found to be compatible with the idea that vasopressin exerted an influence on the shunt pathway of the frog skin, and this effect of vasopressin could be reversed by amiloride.", "PMID": 961856}
{"id": "pubmed23n0032_4700", "title": "Rise in plasma alpha-amino nitrogen concentration in rats eviscerated after colure regulation.", "content": "Rats were exposed to 4 or 25 degrees C for 6, 12, and 24 h or for longer periods up to 2 wk, then eviscerated. The hourly changes in plasma alpha-amino nitrogen were followed for 4 h after operation, which because of functional removal of the liver allows amino acids released from the extrahepatic tissues to accumulate in the blood. Although cold exposure of tissues to accumulate in the blood. Although cold exposure of 24-h-fasted rats for 6 h before eviscerated resulted in an increased rate of rise of plasma alpha-amino nitrogen postoperatively, the rise was not significantly greater than warm controls until after 12 h of exposure. Thereafter, similar results were obtained whether cold was 12, 24, or 48 h or 14 days duration. Fasting in the cold produced an immediate sharp rise after evisceration so that the concentration was 2 times the control value in 2 h. By contrast, animals allowed to eat adlibitum showed the rise after evisceration but it was progressively smaller in amount as the exposure extended beyond the 1st day. After 5 days there were no longer significant differences from control values. The reduction in rate of rise in plasma alpha-amino nitrogen coincided with cold-induced increases in food intake. The findings support the view that protein metabolism of muscle, the predominant extrahepatic tissue, may participate in thermogenesis when other sources of energy in the body have been depleted.", "contents": "Rise in plasma alpha-amino nitrogen concentration in rats eviscerated after colure regulation. Rats were exposed to 4 or 25 degrees C for 6, 12, and 24 h or for longer periods up to 2 wk, then eviscerated. The hourly changes in plasma alpha-amino nitrogen were followed for 4 h after operation, which because of functional removal of the liver allows amino acids released from the extrahepatic tissues to accumulate in the blood. Although cold exposure of tissues to accumulate in the blood. Although cold exposure of 24-h-fasted rats for 6 h before eviscerated resulted in an increased rate of rise of plasma alpha-amino nitrogen postoperatively, the rise was not significantly greater than warm controls until after 12 h of exposure. Thereafter, similar results were obtained whether cold was 12, 24, or 48 h or 14 days duration. Fasting in the cold produced an immediate sharp rise after evisceration so that the concentration was 2 times the control value in 2 h. By contrast, animals allowed to eat adlibitum showed the rise after evisceration but it was progressively smaller in amount as the exposure extended beyond the 1st day. After 5 days there were no longer significant differences from control values. The reduction in rate of rise in plasma alpha-amino nitrogen coincided with cold-induced increases in food intake. The findings support the view that protein metabolism of muscle, the predominant extrahepatic tissue, may participate in thermogenesis when other sources of energy in the body have been depleted.", "PMID": 961857}
{"id": "pubmed23n0032_4701", "title": "Reversal of hypoxic bradycardia by halothane or midcollicular decerebration.", "content": "Previous work suggests a primary vagal bradycardia during hypoxic excitation of the arterial chemoreceptors. In this study we examined whether tachycardia, resulting from withdrawal of vagal inhibition of the cardiac pacemaker, may also occur during hypoxia. Cats with cervical spinal section were ventilated with hypoxic gas mixtures or allowed to remain apneic in expiration while heart rate was continually monitored. During recovery from halothane anesthesia or following electrolytic midcollicular decerebration, the bradycardic response to hypoxic or asphyxic stimulation was reduced or was reversed to tachycardia. The extent of reduction or reversal of the bradycardia was positively correlated with the level of the arterial pressure which was adjusted by either hemorrhage or infusion of phenylephrine. These findings indicate a tachycardic component of the vagal response to hypoxia, integrated in the pontomedullary region of the brainstem and resulting from interaction between the chemoreceptor and baroreceptor reflexes.", "contents": "Reversal of hypoxic bradycardia by halothane or midcollicular decerebration. Previous work suggests a primary vagal bradycardia during hypoxic excitation of the arterial chemoreceptors. In this study we examined whether tachycardia, resulting from withdrawal of vagal inhibition of the cardiac pacemaker, may also occur during hypoxia. Cats with cervical spinal section were ventilated with hypoxic gas mixtures or allowed to remain apneic in expiration while heart rate was continually monitored. During recovery from halothane anesthesia or following electrolytic midcollicular decerebration, the bradycardic response to hypoxic or asphyxic stimulation was reduced or was reversed to tachycardia. The extent of reduction or reversal of the bradycardia was positively correlated with the level of the arterial pressure which was adjusted by either hemorrhage or infusion of phenylephrine. These findings indicate a tachycardic component of the vagal response to hypoxia, integrated in the pontomedullary region of the brainstem and resulting from interaction between the chemoreceptor and baroreceptor reflexes.", "PMID": 961858}
{"id": "pubmed23n0032_4702", "title": "Influence of medium tonicity on respiration by suspensions of human platelets.", "content": "We describe the use of graded decrements of medium osmolarity to progressively unmask respiratory capacities of whole human platelets in suspension. This departure led to the first demonstration that human platelet mitochondria are capable of tightly coupled respiration that responds to addition of mitochondrial substrates, ADP, and inhibitors in a way that other mammalian mitochondria are expected to behave. In 300 mosM media added alpha-glycerophosphate (G3P), succinate, or ADP effected only slight stimulation of base-line O2 consumption. At 180 mosM O2 consumption peaked and was not significantly affected by succinate or ADP. At 80 mosM base-line O2 consumption fell precipitously and was restored by G3P or succinate prior to being raised to its highest levels by ADP. Added NADH had no effect on O2 consumption at 80 mosM but sharply stimulated it when platelet suspensions were exposed to 60 mosM media by pretreatment with distilled water. At 80 mosM, selected compounds that inhibit or uncouple oxidative phosphorylation of isolated mammalian mitochondria from a variety of cells exerted similar influences on while platelets.", "contents": "Influence of medium tonicity on respiration by suspensions of human platelets. We describe the use of graded decrements of medium osmolarity to progressively unmask respiratory capacities of whole human platelets in suspension. This departure led to the first demonstration that human platelet mitochondria are capable of tightly coupled respiration that responds to addition of mitochondrial substrates, ADP, and inhibitors in a way that other mammalian mitochondria are expected to behave. In 300 mosM media added alpha-glycerophosphate (G3P), succinate, or ADP effected only slight stimulation of base-line O2 consumption. At 180 mosM O2 consumption peaked and was not significantly affected by succinate or ADP. At 80 mosM base-line O2 consumption fell precipitously and was restored by G3P or succinate prior to being raised to its highest levels by ADP. Added NADH had no effect on O2 consumption at 80 mosM but sharply stimulated it when platelet suspensions were exposed to 60 mosM media by pretreatment with distilled water. At 80 mosM, selected compounds that inhibit or uncouple oxidative phosphorylation of isolated mammalian mitochondria from a variety of cells exerted similar influences on while platelets.", "PMID": 961859}
{"id": "pubmed23n0032_4703", "title": "Fever and antipyresis in the lizard Dipsosaurus dorsalis.", "content": "Lizards (Dipsosaurus dorsalis) were placed in a desertlike environment in which the ambient temperature (Ta) at night (1800-0600 h) was 12 degrees C and the day (0600-1800 h) Ta was between 30 and 55 degrees C depending on the location within the chamber. When dead Aeromonas hydrophila (4 X 10(9) organisms) was injected into nine lizards, an elevation in body temperature (Tb) of 2.7 degrees C was observed during the same day. On the day after bacterial injection the lizards' body temperatures averaged 41.6 degrees C, an increase of 4.2 degrees C over their control day Tb. Further investigations on the febrile response of D. dorsalis were conducted at the University of Wisconsin's Biotron, where there exists a simulated desert environment with the light intensity, temperature, and humidity closely parelleling a typical spring day in the southwestern desert of the United States (the natural habitat of Dipsosaurus). In this environment injection of dead bacteria into seven lizards led to an average febrile response of similar magnitude (Tb = 40.5 degrees C) but with a longer latency than that found at the University of Michigan. Injection of 13 lizards with live A. hydrophila (5 X 10(9) organism subcut.) in the simulated desert at Michigan led to a daytime fever averaging 2.3 degrees C (mean Tb = 40.6 degrees C) over a 5-day period. During the 6th and 7th day the lizards' body temperature returned to the normal or afebrile level. Injections of sodium salicylate along with dead A. hydrophila resulted in a dose-dependent attenuation of the febrile response. These results demonstrate that the reptilian febrile response is strikingly similar to avian and mammalian fever and suggest a common origin and perhaps function for the febrile mechanism.", "contents": "Fever and antipyresis in the lizard Dipsosaurus dorsalis. Lizards (Dipsosaurus dorsalis) were placed in a desertlike environment in which the ambient temperature (Ta) at night (1800-0600 h) was 12 degrees C and the day (0600-1800 h) Ta was between 30 and 55 degrees C depending on the location within the chamber. When dead Aeromonas hydrophila (4 X 10(9) organisms) was injected into nine lizards, an elevation in body temperature (Tb) of 2.7 degrees C was observed during the same day. On the day after bacterial injection the lizards' body temperatures averaged 41.6 degrees C, an increase of 4.2 degrees C over their control day Tb. Further investigations on the febrile response of D. dorsalis were conducted at the University of Wisconsin's Biotron, where there exists a simulated desert environment with the light intensity, temperature, and humidity closely parelleling a typical spring day in the southwestern desert of the United States (the natural habitat of Dipsosaurus). In this environment injection of dead bacteria into seven lizards led to an average febrile response of similar magnitude (Tb = 40.5 degrees C) but with a longer latency than that found at the University of Michigan. Injection of 13 lizards with live A. hydrophila (5 X 10(9) organism subcut.) in the simulated desert at Michigan led to a daytime fever averaging 2.3 degrees C (mean Tb = 40.6 degrees C) over a 5-day period. During the 6th and 7th day the lizards' body temperature returned to the normal or afebrile level. Injections of sodium salicylate along with dead A. hydrophila resulted in a dose-dependent attenuation of the febrile response. These results demonstrate that the reptilian febrile response is strikingly similar to avian and mammalian fever and suggest a common origin and perhaps function for the febrile mechanism.", "PMID": 961860}
{"id": "pubmed23n0032_4704", "title": "Responses of rat brainstem neurons to carotid occlusion.", "content": "The distribution and features of cardiovascular neurons (CVN) were studied in the brainstem of chloralose-anesthetized rats. Only neurons yielding an immediate response to carotid occulsion (CO) were considered as CVN. The criterion was deduced from the response patterns of cardiovascular afferent (carotid sinus nerve) and efferents (sympathetic and vagal nerves) during CO. Seventy-two CVN were identified and were observed to be mostly in the medullarly reticular formation and to some extent in the nucleus tractus solitarius and nucleus ambiguus. Fifty-five CVN (type A) decreased their firing rates in response to CO where 17 CVN (type B) increased their rates. Analyses of the time courses of their responses to CO revealed the convergence of the chemoreceptor and baroreceptor inputs onto some type A neurons. Electrical stimulation of the spinal cord evoked orthodromic responses in some neurons in both types. The evoked responses were blocked by CO in type A neurons whereas they were facilitated in those of type B. These results suggest that input from arterial baroreceptors, arterial chemoreceptors, and spinal ascending afferents is integrated within CVN.", "contents": "Responses of rat brainstem neurons to carotid occlusion. The distribution and features of cardiovascular neurons (CVN) were studied in the brainstem of chloralose-anesthetized rats. Only neurons yielding an immediate response to carotid occulsion (CO) were considered as CVN. The criterion was deduced from the response patterns of cardiovascular afferent (carotid sinus nerve) and efferents (sympathetic and vagal nerves) during CO. Seventy-two CVN were identified and were observed to be mostly in the medullarly reticular formation and to some extent in the nucleus tractus solitarius and nucleus ambiguus. Fifty-five CVN (type A) decreased their firing rates in response to CO where 17 CVN (type B) increased their rates. Analyses of the time courses of their responses to CO revealed the convergence of the chemoreceptor and baroreceptor inputs onto some type A neurons. Electrical stimulation of the spinal cord evoked orthodromic responses in some neurons in both types. The evoked responses were blocked by CO in type A neurons whereas they were facilitated in those of type B. These results suggest that input from arterial baroreceptors, arterial chemoreceptors, and spinal ascending afferents is integrated within CVN.", "PMID": 961861}
{"id": "pubmed23n0032_4705", "title": "Effects of 4-pentenoic acid on renal phosphate and calcium excretion in the dog.", "content": "The effects of 4-pentenoic acid (4-PA) on renal excretion of phosphate and calcium were studied in anesthetized mongrel dogs. The major metabolic action of 4-PA is inhibition of long-chain fatty acid oxidation. In intact dogs undergoing modest saline diuresis, 4-PA caused significant decrease in the percentage of filtered phosphate reabsorbed. No statistically significant calciuria was observed. Similar results were observed in intact dogs undergoing more brisk saline diuresis as well as in acutely and chronically thyroparathyroidectomized dogs. However, when results were pooled, both phosphate and calcium excretions were significantly increased. The results are interpreted as demonstrating the importance of fatty acid oxidation in providing energy for the work of tubular phosphate reabsorption. Either the energy source for calcium reabsorption differs from fatty acid oxodation, or calcium reabsorption is less sensitive to changes in energy sources. The action of 4-PA is independent of the forces involved in saline diuresis and of parathyroid hormone.", "contents": "Effects of 4-pentenoic acid on renal phosphate and calcium excretion in the dog. The effects of 4-pentenoic acid (4-PA) on renal excretion of phosphate and calcium were studied in anesthetized mongrel dogs. The major metabolic action of 4-PA is inhibition of long-chain fatty acid oxidation. In intact dogs undergoing modest saline diuresis, 4-PA caused significant decrease in the percentage of filtered phosphate reabsorbed. No statistically significant calciuria was observed. Similar results were observed in intact dogs undergoing more brisk saline diuresis as well as in acutely and chronically thyroparathyroidectomized dogs. However, when results were pooled, both phosphate and calcium excretions were significantly increased. The results are interpreted as demonstrating the importance of fatty acid oxidation in providing energy for the work of tubular phosphate reabsorption. Either the energy source for calcium reabsorption differs from fatty acid oxodation, or calcium reabsorption is less sensitive to changes in energy sources. The action of 4-PA is independent of the forces involved in saline diuresis and of parathyroid hormone.", "PMID": 961862}
{"id": "pubmed23n0032_4706", "title": "Further studies on coupling between sodium transport and respiration in toad urinary bladder.", "content": "When sodium transport (JNa) and CO2 production (JCO2) were measured simultaneously in the toad urinary bladder in the absence of electrochemical gradients under conditions of spontaneous variation of JNa, the curve of JNa on JCO2, phiJNa/phiJCO2, was found to be highly linear in an individual epithelium. However, no unique value appeared to characterize a population of bladders. In an effort to investigate the nature of this variation, phiJNa/phiJCO2 was examined under a variety of conditions. It was found that agents that affect sodium entry into the active transport pool, e.g. vasopressin and amiloride, or those that influence the energy-linked exit step, e.g. ouabain and insulin, have no effect on the phiJNa/phiJCO2. To investigate the possibility of the presence of a significant backleak from the serosal side into the cell, the sodium concentration was changed to 75 and 160 mM. Neither of these maneuvers influenced phiJNa/phiJCO2 or (JCO2)JNa=O. Furthermore, in the absence of mucosal sodium, ouabain had no effect on JCO2, suggesting that no significant recirculation is occurring. It is concluded that for each individual epithelium JNa is coupled to JCO2, and their ratios appear, within experimental error, to be invariant. It is suggested that sodium traverses the active transport pathway largely or entirely in one direction.", "contents": "Further studies on coupling between sodium transport and respiration in toad urinary bladder. When sodium transport (JNa) and CO2 production (JCO2) were measured simultaneously in the toad urinary bladder in the absence of electrochemical gradients under conditions of spontaneous variation of JNa, the curve of JNa on JCO2, phiJNa/phiJCO2, was found to be highly linear in an individual epithelium. However, no unique value appeared to characterize a population of bladders. In an effort to investigate the nature of this variation, phiJNa/phiJCO2 was examined under a variety of conditions. It was found that agents that affect sodium entry into the active transport pool, e.g. vasopressin and amiloride, or those that influence the energy-linked exit step, e.g. ouabain and insulin, have no effect on the phiJNa/phiJCO2. To investigate the possibility of the presence of a significant backleak from the serosal side into the cell, the sodium concentration was changed to 75 and 160 mM. Neither of these maneuvers influenced phiJNa/phiJCO2 or (JCO2)JNa=O. Furthermore, in the absence of mucosal sodium, ouabain had no effect on JCO2, suggesting that no significant recirculation is occurring. It is concluded that for each individual epithelium JNa is coupled to JCO2, and their ratios appear, within experimental error, to be invariant. It is suggested that sodium traverses the active transport pathway largely or entirely in one direction.", "PMID": 961863}
{"id": "pubmed23n0032_4707", "title": "Propagation of stimulated slow waves in cat intestinal muscle.", "content": "This study was designed to examine the propagation and propagation failure of the slow wave. Spontaneous slow waves and action potentials were recorded from the longitudinal muscle layer of small strips of cat jejunum. Slow waves could be stimulated electrically at intervals shorter than the spontaneous interval, and the velocity of propagation of these slow waves was slower than the spontaneous slow waves. The velocity of propagation was approximately proportional to the time between slow waves. At intervals about one-half the normal spontaneous interval the propagation became slow and unstable, which would result in the periodic failure of a single slow wave. This type of failure may underlie the formation of the slow-wave frequency gradient observed in the intact intestine. The wave shape of the external recording of the slow wave, together with the measurement of electrotonic current spread in strips of isolated muscle, suggest that the slow wave propagates by local circuit currents in an electrically excitable cablelike tissue.", "contents": "Propagation of stimulated slow waves in cat intestinal muscle. This study was designed to examine the propagation and propagation failure of the slow wave. Spontaneous slow waves and action potentials were recorded from the longitudinal muscle layer of small strips of cat jejunum. Slow waves could be stimulated electrically at intervals shorter than the spontaneous interval, and the velocity of propagation of these slow waves was slower than the spontaneous slow waves. The velocity of propagation was approximately proportional to the time between slow waves. At intervals about one-half the normal spontaneous interval the propagation became slow and unstable, which would result in the periodic failure of a single slow wave. This type of failure may underlie the formation of the slow-wave frequency gradient observed in the intact intestine. The wave shape of the external recording of the slow wave, together with the measurement of electrotonic current spread in strips of isolated muscle, suggest that the slow wave propagates by local circuit currents in an electrically excitable cablelike tissue.", "PMID": 961864}
{"id": "pubmed23n0032_4708", "title": "Determinants of glomerular filtration rate in the dog.", "content": "Micropuncture techniques were used to evaluate the determinants of glomerular filtration in hydropenic dogs. Stop-flow and servo-null techniques were used to estimate the glomerular capillary and proximal tubule hydrostatic pressures. The validity of stop-flow estimates was verified by comparisons with direct puncture of glomerular capillaries in Munich-Wistar rats. Efferent arteriolar oncotic pressure was calculated from the filtration fraction and systemic protein concentrations. This calculation was verified in separate experiments by measurement of the protein concentration in blood collected directly from efferent arterioles. In 14 dogs, estimated glomerular capillary pressure (GCP) averaged 65.8 +/- 2.9 mmHg and proximal tubule pressure (PT) averaged 20.5 +/- 1.3 mmHg. The net hydrostatic filtration pressure (GCP - PT) of 45.3 +/- 2.7 mmHg was significantly higher than the efferent arteriolar oncotic pressure (piEA) of 33.2 +/- 2.8 mmHg (P less than .001). These findings indicate that filtration dynamics in the dog are characterized by filtration pressure disequilibrium.", "contents": "Determinants of glomerular filtration rate in the dog. Micropuncture techniques were used to evaluate the determinants of glomerular filtration in hydropenic dogs. Stop-flow and servo-null techniques were used to estimate the glomerular capillary and proximal tubule hydrostatic pressures. The validity of stop-flow estimates was verified by comparisons with direct puncture of glomerular capillaries in Munich-Wistar rats. Efferent arteriolar oncotic pressure was calculated from the filtration fraction and systemic protein concentrations. This calculation was verified in separate experiments by measurement of the protein concentration in blood collected directly from efferent arterioles. In 14 dogs, estimated glomerular capillary pressure (GCP) averaged 65.8 +/- 2.9 mmHg and proximal tubule pressure (PT) averaged 20.5 +/- 1.3 mmHg. The net hydrostatic filtration pressure (GCP - PT) of 45.3 +/- 2.7 mmHg was significantly higher than the efferent arteriolar oncotic pressure (piEA) of 33.2 +/- 2.8 mmHg (P less than .001). These findings indicate that filtration dynamics in the dog are characterized by filtration pressure disequilibrium.", "PMID": 961865}
{"id": "pubmed23n0032_4709", "title": "Maturation of contractile response of ductus arteriosus to oxygen and drugs.", "content": "Contractile responses of rings of ductus arteriosus from fetal and neonatal guinea pigs were studied in buffered Krebs-Henseleit solutions of various oxygen tensions. Oxygen-induced contraction of ductus arteriosus increased with gestational age, peaking at term and attenuating within 24 h after birth. Contractions of ductus in response to potassium were not different in term and preterm fetuses. Maximal contractile response of pre- and postductal aortic rings to oxygen was 8.3% of the maximal oxygen-induced contraction of ductal rings from the same fetuses. Pulmonary artery was quite insensitive to oxygen. Of 12 ductus exposed to bradykinin in the absence of oxygen seven contracted (maximal response was obtained with 15.0 ng/ml). Exposure of ductus to bradykinin in the absence of oxygen enhanced subsequent contractions of ductal smooth muscle rings to air. Atropine failed to inhibit the oxygen-induced contraction of ductus. These data suggest that acetylcholine is not essential for oxygen-induced contraction of the guinea pig ductus arteriosus.", "contents": "Maturation of contractile response of ductus arteriosus to oxygen and drugs. Contractile responses of rings of ductus arteriosus from fetal and neonatal guinea pigs were studied in buffered Krebs-Henseleit solutions of various oxygen tensions. Oxygen-induced contraction of ductus arteriosus increased with gestational age, peaking at term and attenuating within 24 h after birth. Contractions of ductus in response to potassium were not different in term and preterm fetuses. Maximal contractile response of pre- and postductal aortic rings to oxygen was 8.3% of the maximal oxygen-induced contraction of ductal rings from the same fetuses. Pulmonary artery was quite insensitive to oxygen. Of 12 ductus exposed to bradykinin in the absence of oxygen seven contracted (maximal response was obtained with 15.0 ng/ml). Exposure of ductus to bradykinin in the absence of oxygen enhanced subsequent contractions of ductal smooth muscle rings to air. Atropine failed to inhibit the oxygen-induced contraction of ductus. These data suggest that acetylcholine is not essential for oxygen-induced contraction of the guinea pig ductus arteriosus.", "PMID": 961866}
{"id": "pubmed23n0032_4710", "title": "Intestinal ion transport: effect of norepinephrine, pilocarpine, and atropine.", "content": "The effects of parenteral pilocarpine, atropine, and norepinephrine on salt and water transport were studied in jejunum and ileum of anesthetized rats. Pilocarpine increased jejunal transmural PD, reduced absorption of Na, K, HCO3, and H2O, and increased secretion of Cl; in ileum, it caused secretion of Na and H2O, elicited secretion of K, and reduced the absorption of Cl. In both segments, perfusate became more akaline, and there was less of a rise in PCO2. Atropine prevented all changes caused by pilocarpine. Atropine alone increased jejunal absorption of Na and HCO3 and acidity of perfusate, implying that cholinergic nerves influence transport. Norepinephrine augmented jejunal absorption of Na, Cl, and H2O but caused no change in PD. In ileum, norepinephrine increased absorption of Na and Cl, reduced the rise in pH, increased the rise in PCO2 of perfusate, but did not affect net HCO3 movement. With all agents, when Na absorption increased, perfusate became more acidic in jejunum and less alkaline in ileum, evidence of an association between Na and H transport.", "contents": "Intestinal ion transport: effect of norepinephrine, pilocarpine, and atropine. The effects of parenteral pilocarpine, atropine, and norepinephrine on salt and water transport were studied in jejunum and ileum of anesthetized rats. Pilocarpine increased jejunal transmural PD, reduced absorption of Na, K, HCO3, and H2O, and increased secretion of Cl; in ileum, it caused secretion of Na and H2O, elicited secretion of K, and reduced the absorption of Cl. In both segments, perfusate became more akaline, and there was less of a rise in PCO2. Atropine prevented all changes caused by pilocarpine. Atropine alone increased jejunal absorption of Na and HCO3 and acidity of perfusate, implying that cholinergic nerves influence transport. Norepinephrine augmented jejunal absorption of Na, Cl, and H2O but caused no change in PD. In ileum, norepinephrine increased absorption of Na and Cl, reduced the rise in pH, increased the rise in PCO2 of perfusate, but did not affect net HCO3 movement. With all agents, when Na absorption increased, perfusate became more acidic in jejunum and less alkaline in ileum, evidence of an association between Na and H transport.", "PMID": 961867}
{"id": "pubmed23n0032_4711", "title": "Effect of vasopressin on sodium excretion and plasma antinatriferic activity in the dog.", "content": "Vasopressin (VP) was administered for 1 h intravenously to hydropenic, anesthetized dogs in doses of 1.0-1.25 mU/kg per min. In 14 experiments, sodium excretion (UNA V) increased from a mean of 13 +/- 5 to a peak of 96 +/- 21 mueq/min 40 min after beginning infusion (P less than .001). Urine flow and potassium excretion increased from 0.18 +/-.04 ml/min and 20 +/- 2 meuq/min to peak values of 0.6 +/- .08 ml/min and 61 +/- 9 mueq/min, respectively (P less than .001), with no significant increase in glomerular filtration rate. No significant changes in UNA V occurred in eight sham control experiments of in six experiments in which VP was given at 75 muU/kf per min. To test the hypothesis that VP might be natriuretic indirectly by releasing a natriuretic substance, plasms ultrafiltrates were tested for toad bladder antinatriferic activity(AA). During steady-state control, AA was -10 +/- 3%. Thirty and sixty minutes after beginning VP, AA increased to -24 +/- 3% (P less than .05) and -26 +/- 2% (P less than .001), respectiviely. No significant change in plasma AA occurred in either sham controls or in animals given the subnatriuretic VP dose. Incubation of plasma with 1,000 muU/ml VP caused no increase in AA. The data show that VP natriuresis is accompanied by an increase in plasms AA. The results suggest that vasopressin natriuresis in hydropenic dogs at least in part to the release of a humoral inhibitor of renal tubular sodium transport.", "contents": "Effect of vasopressin on sodium excretion and plasma antinatriferic activity in the dog. Vasopressin (VP) was administered for 1 h intravenously to hydropenic, anesthetized dogs in doses of 1.0-1.25 mU/kg per min. In 14 experiments, sodium excretion (UNA V) increased from a mean of 13 +/- 5 to a peak of 96 +/- 21 mueq/min 40 min after beginning infusion (P less than .001). Urine flow and potassium excretion increased from 0.18 +/-.04 ml/min and 20 +/- 2 meuq/min to peak values of 0.6 +/- .08 ml/min and 61 +/- 9 mueq/min, respectively (P less than .001), with no significant increase in glomerular filtration rate. No significant changes in UNA V occurred in eight sham control experiments of in six experiments in which VP was given at 75 muU/kf per min. To test the hypothesis that VP might be natriuretic indirectly by releasing a natriuretic substance, plasms ultrafiltrates were tested for toad bladder antinatriferic activity(AA). During steady-state control, AA was -10 +/- 3%. Thirty and sixty minutes after beginning VP, AA increased to -24 +/- 3% (P less than .05) and -26 +/- 2% (P less than .001), respectiviely. No significant change in plasma AA occurred in either sham controls or in animals given the subnatriuretic VP dose. Incubation of plasma with 1,000 muU/ml VP caused no increase in AA. The data show that VP natriuresis is accompanied by an increase in plasms AA. The results suggest that vasopressin natriuresis in hydropenic dogs at least in part to the release of a humoral inhibitor of renal tubular sodium transport.", "PMID": 961868}
{"id": "pubmed23n0032_4712", "title": "Effect of serotonin on brown adipose tissue and on its sympathetic neurons.", "content": "Previously we have shown that there are two types of sympathetic neurons in the rat interscapular brown fat-pad: long adrenergic neurons with cell bodies located in the paraspinal ganglia and short adrenergic neurons with cell bodies located within the pad itself. The present studies examine 1) the uptake of [3H]norepinephrine by these neurons, 2) the effect of serotonin on release of the catecholamine, and 3) the metabolic action of serotonin on brown fat. [3H]norepinephrine uptake from the medium in which brown fat slices are incubated is linear with time up to 90 min. This uptake is blocked by both cocaine and imipramine. As these drugs block uptake by sympathetic neurons, the [3H]norepinephrine is probably taken up by neurons and not by some other cell in the pad. Serotonin creatinine sulfate, but not creatinine sulfate, promotes the release of the catecholamine from these neurons. Serotonin also exerts a norepinephrine-like metabolic effect on slices of normal brown fat. It stimulates lipolysis and inhibits lipogenesis. Serotonin has no effect on isolated brown fat cells or on slices of reserpinized tissue. It also does not potentiate the action of norepinephrine on these last two preparations. Therefore serotonin's action on brown fat is mediated by norepinephrine-containing stromal cells. The data suggest that the rat brown fat-pad has sympathetic neurons that can take up norepinephrine and that serotonin exerts its metabolic effect by stimulating the release of norepinephrine from these neurons.", "contents": "Effect of serotonin on brown adipose tissue and on its sympathetic neurons. Previously we have shown that there are two types of sympathetic neurons in the rat interscapular brown fat-pad: long adrenergic neurons with cell bodies located in the paraspinal ganglia and short adrenergic neurons with cell bodies located within the pad itself. The present studies examine 1) the uptake of [3H]norepinephrine by these neurons, 2) the effect of serotonin on release of the catecholamine, and 3) the metabolic action of serotonin on brown fat. [3H]norepinephrine uptake from the medium in which brown fat slices are incubated is linear with time up to 90 min. This uptake is blocked by both cocaine and imipramine. As these drugs block uptake by sympathetic neurons, the [3H]norepinephrine is probably taken up by neurons and not by some other cell in the pad. Serotonin creatinine sulfate, but not creatinine sulfate, promotes the release of the catecholamine from these neurons. Serotonin also exerts a norepinephrine-like metabolic effect on slices of normal brown fat. It stimulates lipolysis and inhibits lipogenesis. Serotonin has no effect on isolated brown fat cells or on slices of reserpinized tissue. It also does not potentiate the action of norepinephrine on these last two preparations. Therefore serotonin's action on brown fat is mediated by norepinephrine-containing stromal cells. The data suggest that the rat brown fat-pad has sympathetic neurons that can take up norepinephrine and that serotonin exerts its metabolic effect by stimulating the release of norepinephrine from these neurons.", "PMID": 961869}
{"id": "pubmed23n0032_4713", "title": "Effect of insulin on canalicular bile formation.", "content": "Mongrel dogs were prepared by cholecystectomy, ligation of the lesser pancreatic duct, and insertion of gastric and duodenal cannulas. The common bile duct was cannulated through the duodenal fistula. After bile flow had been stabilized by intravenous infusion of sodium taurocholate the dogs were given an intravenous injection of insulin or 0.9% NaCl (control). Insulin caused marked increases in bile flow, chloride output, and biliary clearance of erythritol and small increases in bicarbonate output and bile salt output. The increased erythritol clearance indicates that canalicular secretion contributes to insulin choleresis in dogs.", "contents": "Effect of insulin on canalicular bile formation. Mongrel dogs were prepared by cholecystectomy, ligation of the lesser pancreatic duct, and insertion of gastric and duodenal cannulas. The common bile duct was cannulated through the duodenal fistula. After bile flow had been stabilized by intravenous infusion of sodium taurocholate the dogs were given an intravenous injection of insulin or 0.9% NaCl (control). Insulin caused marked increases in bile flow, chloride output, and biliary clearance of erythritol and small increases in bicarbonate output and bile salt output. The increased erythritol clearance indicates that canalicular secretion contributes to insulin choleresis in dogs.", "PMID": 961870}
{"id": "pubmed23n0032_4714", "title": "Effect of potassium on ammoniagenesis by renal mitochondria.", "content": "Ammonia production by rat renal cortical mitochondria was studied with both in vivo and in vitro manipulation of potassium to further elucidate the mechanisms relating potassium homeostasis and renal ammonia production. Mitochondria from potassium-depleted animals demonstrated an increase in ammonia production at all glutamine concentrations studied, which ranged from 0.5 to 10 mM. This increase in ammoniagenesis compared favorably in degree of change with and was of sufficient absolute magnitude to entirely account for the findings observed both in renal cortical slices studied in vitro and in the intact animal. When rotenone is added to the medium, increased ammoniagenesis is still detected, indicating that either glutamine entry into the mitochondria and/or the activity of phosphate-dependent glutaminase are critical rate-controlling steps. In contrast to studies with renal slices, a decrease in ammonia production was not apparent with cortical mitochondria from chronically potassium-loaded animals. In vitro alterations of the potassium homeostasis. Therefore, these experimental manipulations either do not activate an effector mechanism which takes place in vivo, or the alteration in ammoniagenesis requires an adaptation over time that is not achieved with this in vitro approach.", "contents": "Effect of potassium on ammoniagenesis by renal mitochondria. Ammonia production by rat renal cortical mitochondria was studied with both in vivo and in vitro manipulation of potassium to further elucidate the mechanisms relating potassium homeostasis and renal ammonia production. Mitochondria from potassium-depleted animals demonstrated an increase in ammonia production at all glutamine concentrations studied, which ranged from 0.5 to 10 mM. This increase in ammoniagenesis compared favorably in degree of change with and was of sufficient absolute magnitude to entirely account for the findings observed both in renal cortical slices studied in vitro and in the intact animal. When rotenone is added to the medium, increased ammoniagenesis is still detected, indicating that either glutamine entry into the mitochondria and/or the activity of phosphate-dependent glutaminase are critical rate-controlling steps. In contrast to studies with renal slices, a decrease in ammonia production was not apparent with cortical mitochondria from chronically potassium-loaded animals. In vitro alterations of the potassium homeostasis. Therefore, these experimental manipulations either do not activate an effector mechanism which takes place in vivo, or the alteration in ammoniagenesis requires an adaptation over time that is not achieved with this in vitro approach.", "PMID": 961871}
{"id": "pubmed23n0032_4715", "title": "Mineralocorticoid activity of 18-OH-DOC and DOCA in dogs with an aortic-caval fistula.", "content": "The mineralocorticoid activity of 18-hydroxydeoxycorticosterone (18-OH-DOC) was measured during chronic electrolyte balance studies or during the postprandial increase in electrolyte excretion in conscious dogs with an aortic-caval fistula. In the chronic balance study, daily doses of DOCA and 18-OH-DOC ranging from 1 to 25 mg were administered intramuscularly for 3 consecutive days each, 6 h prior to feeding. For the postprandial study, 2-10 mg of DOCA and 18-OH-DOC were administered at feeding and postprandial electrolyte excretion was measured hourly for 6 h. Sodium and fluid retention in the dogs with an aortic-caval fistula was related to the dose of mineralocorticoid administered and equivalent sodium-retaining responses were achieved with 6-10 times more 18-OH-DOC than DOCA. Rapid absorption of both steroids was suggested from the postprandial measurements of urinary sodium excretion. Slight potassium retention occurred during the chronic administration of large doses of DOCA but the postprandial potassium responses produced by the steroids were variable and suggested a slight kaliuresis. The data also emphasize the importance of mineralocorticoids in sodium retention and ascites formation in this experimental model of high-output heart failure.", "contents": "Mineralocorticoid activity of 18-OH-DOC and DOCA in dogs with an aortic-caval fistula. The mineralocorticoid activity of 18-hydroxydeoxycorticosterone (18-OH-DOC) was measured during chronic electrolyte balance studies or during the postprandial increase in electrolyte excretion in conscious dogs with an aortic-caval fistula. In the chronic balance study, daily doses of DOCA and 18-OH-DOC ranging from 1 to 25 mg were administered intramuscularly for 3 consecutive days each, 6 h prior to feeding. For the postprandial study, 2-10 mg of DOCA and 18-OH-DOC were administered at feeding and postprandial electrolyte excretion was measured hourly for 6 h. Sodium and fluid retention in the dogs with an aortic-caval fistula was related to the dose of mineralocorticoid administered and equivalent sodium-retaining responses were achieved with 6-10 times more 18-OH-DOC than DOCA. Rapid absorption of both steroids was suggested from the postprandial measurements of urinary sodium excretion. Slight potassium retention occurred during the chronic administration of large doses of DOCA but the postprandial potassium responses produced by the steroids were variable and suggested a slight kaliuresis. The data also emphasize the importance of mineralocorticoids in sodium retention and ascites formation in this experimental model of high-output heart failure.", "PMID": 961872}
{"id": "pubmed23n0032_4716", "title": "Effects of digitalis and calcium on papillary muscles in normal and hypoxic states.", "content": "The effects of digoxin and ouabain in 2.5 and 4.0 mM extracellular calcium were studied in well-oxygenated and hypoxic isolated, isometrically contracting cat papillary muscles. Muscle digoxin content was measured at the conclusion of the digoxin experiments. In the well-oxygenated environment muscles in the higher Ca bathing media reached peak glycoside inotropic effect sooner and contained 2.7 times more digoxin. During hypoxia and reoxygenation muscles contracting with glycosides performed no differently than those without a glycoside present. Muscle digoxin content was lowered at the end of hypoxia (P less than 0.05) in 2.5 mM Ca; after reoxygenation digoxin content was significantly greater than either before or after hypoxia (P less than 0.001). Hypoxic depression of muscle performance was attenuated in 4.0 mM Ca but muscles in 2.5 mM Ca showed greater improvement during reoxygenation even though the muscles in 4.0 mM Ca had significantly greater digoxin content at the end of reoxygenation (P less than 0.02). It therefore is concluded that, although altered extracellular calcium can alter performance during hypoxia and reoxygenation, muscle performance is not aided by the presence of digitalis and under these conditions performance cannot be correlated with muscle digoxin levels.", "contents": "Effects of digitalis and calcium on papillary muscles in normal and hypoxic states. The effects of digoxin and ouabain in 2.5 and 4.0 mM extracellular calcium were studied in well-oxygenated and hypoxic isolated, isometrically contracting cat papillary muscles. Muscle digoxin content was measured at the conclusion of the digoxin experiments. In the well-oxygenated environment muscles in the higher Ca bathing media reached peak glycoside inotropic effect sooner and contained 2.7 times more digoxin. During hypoxia and reoxygenation muscles contracting with glycosides performed no differently than those without a glycoside present. Muscle digoxin content was lowered at the end of hypoxia (P less than 0.05) in 2.5 mM Ca; after reoxygenation digoxin content was significantly greater than either before or after hypoxia (P less than 0.001). Hypoxic depression of muscle performance was attenuated in 4.0 mM Ca but muscles in 2.5 mM Ca showed greater improvement during reoxygenation even though the muscles in 4.0 mM Ca had significantly greater digoxin content at the end of reoxygenation (P less than 0.02). It therefore is concluded that, although altered extracellular calcium can alter performance during hypoxia and reoxygenation, muscle performance is not aided by the presence of digitalis and under these conditions performance cannot be correlated with muscle digoxin levels.", "PMID": 961873}
{"id": "pubmed23n0032_4717", "title": "Suppressive effect of endotoxin on erythropietin-responsive cells in mice.", "content": "Infection may be associated with failure of erythropoiesis, and endotoxin has been shown to cause a decrease in the number of erythroid cells in the marrow of mice. We have investigated the effect of endotoxin on erythropoiesis in BDF1 mice by studying its effect on the incorporation of intravenously administered 59Fe into peripheral red cells. In normal mice the injection of endotoxin 2 and 3 days prior to the administration of 59Fe results in suppression of its incorporation into red cells. In exhypoxic polycythemic mice, endotoxin suppresses the response to erythropoietin injection when the endotoxin is given prior to or at the same time as the erythropoietin. This effect is much less marked when endotoxin is given after erythropoietin. We conclude that endotoxin either 1) impairs the function of erythropoietin-responsive cells, 2) competively decreases erythroid progenitors by stimulating granulocytes, or 3) inactivates erythropoietin.", "contents": "Suppressive effect of endotoxin on erythropietin-responsive cells in mice. Infection may be associated with failure of erythropoiesis, and endotoxin has been shown to cause a decrease in the number of erythroid cells in the marrow of mice. We have investigated the effect of endotoxin on erythropoiesis in BDF1 mice by studying its effect on the incorporation of intravenously administered 59Fe into peripheral red cells. In normal mice the injection of endotoxin 2 and 3 days prior to the administration of 59Fe results in suppression of its incorporation into red cells. In exhypoxic polycythemic mice, endotoxin suppresses the response to erythropoietin injection when the endotoxin is given prior to or at the same time as the erythropoietin. This effect is much less marked when endotoxin is given after erythropoietin. We conclude that endotoxin either 1) impairs the function of erythropoietin-responsive cells, 2) competively decreases erythroid progenitors by stimulating granulocytes, or 3) inactivates erythropoietin.", "PMID": 961874}
{"id": "pubmed23n0032_4718", "title": "Digestion of tripeptides and disaccharides: relationship with brush border hydrolases.", "content": "Intestinal digestion of two tripeptides (leucyl-glycyl-glycine, prolyl-glycyl-glycine) and two disacchrarides (sucrose, maltose) was examined in the hamster by intestinal perfusion in vivo and hydrolysis of the substrates by microvillus membranes. Perfusion studies showed that luminal disappearance rates of leucyl-glycl-glycine were significantly higher than prolyl-glycyl-glycine (P less than o.001), sucrose (P less than 0.001), and maltose (P less than 0.005). Hydrolytic products of leucyl-glycyl-glycine, sucrose, and maltose were detected in the gut lumen in appreciable concentrations, whereas negligible concentrations of prolyl-glycyl-glycine products were present. Leucyl-glycyl-glycine hydrolysis in microvillus membranes was markedly higher than prolyl-glycyl-glycine (P less than 0.001), which was predominant in the cytoplasmic fraction. These results indicate that leucyl-glycyl-glycine, like sucrose and maltose, is hydrolyzed at the membrane. With some tripeptides, i.e., leucyl-glycyl-glycine, digestion occurs at the microvillus membrane with subsequent transport of hydrolytic products into the intestinal epithelial cell. Other tripeptides, i.e., prolyl-glycyl-glycine, may cross the membrane and undergo intracellular hydrolysis by cytoplasmic peptidases.", "contents": "Digestion of tripeptides and disaccharides: relationship with brush border hydrolases. Intestinal digestion of two tripeptides (leucyl-glycyl-glycine, prolyl-glycyl-glycine) and two disacchrarides (sucrose, maltose) was examined in the hamster by intestinal perfusion in vivo and hydrolysis of the substrates by microvillus membranes. Perfusion studies showed that luminal disappearance rates of leucyl-glycl-glycine were significantly higher than prolyl-glycyl-glycine (P less than o.001), sucrose (P less than 0.001), and maltose (P less than 0.005). Hydrolytic products of leucyl-glycyl-glycine, sucrose, and maltose were detected in the gut lumen in appreciable concentrations, whereas negligible concentrations of prolyl-glycyl-glycine products were present. Leucyl-glycyl-glycine hydrolysis in microvillus membranes was markedly higher than prolyl-glycyl-glycine (P less than 0.001), which was predominant in the cytoplasmic fraction. These results indicate that leucyl-glycyl-glycine, like sucrose and maltose, is hydrolyzed at the membrane. With some tripeptides, i.e., leucyl-glycyl-glycine, digestion occurs at the microvillus membrane with subsequent transport of hydrolytic products into the intestinal epithelial cell. Other tripeptides, i.e., prolyl-glycyl-glycine, may cross the membrane and undergo intracellular hydrolysis by cytoplasmic peptidases.", "PMID": 961875}
{"id": "pubmed23n0032_4719", "title": "Renal handling of [(14)C]mannose.", "content": "The uptake of D-[(14)C]mannose by adult rat kidney cortex slices has been examined. The process involved appeared to be saturable and oxygen dependent and to be inhibited by glucose, galactose, chi-methyl-D-glucoside, and phlorizin. Extensive metabolism of the sugar occurred. (14)CO(2) was produced to an extent comparable to that from D-glucose. Radioactive compounds accumulated in the tissue, approximately 50% of which were in the form of phosphorylated or other polar intermediates. The remaining neutral fraction consisted of mannose with a small amount of label in glucose and an unidentified compound which behaves like an alditol. Mannose concentration in the intracellular fluid was never greater than that of the medium.", "contents": "Renal handling of [(14)C]mannose. The uptake of D-[(14)C]mannose by adult rat kidney cortex slices has been examined. The process involved appeared to be saturable and oxygen dependent and to be inhibited by glucose, galactose, chi-methyl-D-glucoside, and phlorizin. Extensive metabolism of the sugar occurred. (14)CO(2) was produced to an extent comparable to that from D-glucose. Radioactive compounds accumulated in the tissue, approximately 50% of which were in the form of phosphorylated or other polar intermediates. The remaining neutral fraction consisted of mannose with a small amount of label in glucose and an unidentified compound which behaves like an alditol. Mannose concentration in the intracellular fluid was never greater than that of the medium.", "PMID": 961876}
{"id": "pubmed23n0032_4720", "title": "Oral contraceptives, norethindrone and mestranol: effects on tissue levels of minerals.", "content": "The study involved three levels of dietary zinc (deficient, marginal, and adequate) and four hormonal conditions; namely, no steriods, norethindrone, mestranol, and norethindrone plus mestranol. The steroids were incorporated into diets and fed to 11-wk-old female Sprague-Dawley rats. After 10 wk of treatment, various tissues were excised for mineral assays by atomic-absorption spectrophotometry. Both steroids, reduced weight gain. Mestranol depressed plasma zinc, tibia copper and magnesium, and liver iron, but elevated the zinc levels in liver and erythrocytes, plasma copper, liver magnesium and calcium, and iron content of tibia and heart. In general, the effect was most prominent with adequate zinc but diminished in magnitude with the reduction of zinc intake. In addition, norethindrone increased heart iron and tibia calcium. Mestranol appeared to be the main causative factor and may have induced a possible shift of minerals from one pool to another. As expected, zinc deficiency resulted in the reduction of zinc concentrations of plasma, tibia, kidney, and pancreas, and the elevation of copper, iron, magnesium, and calcium concentrations of various tissues.", "contents": "Oral contraceptives, norethindrone and mestranol: effects on tissue levels of minerals. The study involved three levels of dietary zinc (deficient, marginal, and adequate) and four hormonal conditions; namely, no steriods, norethindrone, mestranol, and norethindrone plus mestranol. The steroids were incorporated into diets and fed to 11-wk-old female Sprague-Dawley rats. After 10 wk of treatment, various tissues were excised for mineral assays by atomic-absorption spectrophotometry. Both steroids, reduced weight gain. Mestranol depressed plasma zinc, tibia copper and magnesium, and liver iron, but elevated the zinc levels in liver and erythrocytes, plasma copper, liver magnesium and calcium, and iron content of tibia and heart. In general, the effect was most prominent with adequate zinc but diminished in magnitude with the reduction of zinc intake. In addition, norethindrone increased heart iron and tibia calcium. Mestranol appeared to be the main causative factor and may have induced a possible shift of minerals from one pool to another. As expected, zinc deficiency resulted in the reduction of zinc concentrations of plasma, tibia, kidney, and pancreas, and the elevation of copper, iron, magnesium, and calcium concentrations of various tissues.", "PMID": 961877}
{"id": "pubmed23n0032_4721", "title": "Permeability of renal peritubular capillaries to neutral dextrans dextrans and endogenous albumin.", "content": "Renal lymph-to-renal vein concentration ratios (CL/CV) for neutral dextrans (18-42 A effective radii) and endogenous serum albumin were measured in rats before and during acute colume expansion with isoncotic plasma or Ringer solution. Under all conditions studied, CL/CV decreased with increasing dextran size, in normal hydropenia falling from 0.93 +/- 0.03 SE at 18 A to 0.24 +/- 0.02 a 42 A (n = 12). Albumin (36 A pradius) behaved in a manner similar to a 40 A dextran, CL/CV averaging 0.33 +/- 0.03 (n = 12) in hydropenia. For all dextran sizes studied and for albumin, CL/CV decreased markedly during either form of volume expansion. Ringer loading produced significantly greater decreases in CL/CV for the larger dextrans and albumin than did plasma loading, and also resulted in much greater increases in renal lymph flow, while causing increases in whole kidney fluid reabsorption similar to those with plasma loading. With a compartmental model in which diffusion of dextrans and albumin from capillary lumen to interstitium is opposed by capillary uptake of tubule reabsorbate, these results are interpreted to indicate that connective reflection coefficients for dextrans with radii great than or equal to 36 A and albumin are essentially equal to 1. Indirect evidence is therefore provided that albumin and the larger globulins exert their full osmotic pressures across the walls of peritubular capillaries.", "contents": "Permeability of renal peritubular capillaries to neutral dextrans dextrans and endogenous albumin. Renal lymph-to-renal vein concentration ratios (CL/CV) for neutral dextrans (18-42 A effective radii) and endogenous serum albumin were measured in rats before and during acute colume expansion with isoncotic plasma or Ringer solution. Under all conditions studied, CL/CV decreased with increasing dextran size, in normal hydropenia falling from 0.93 +/- 0.03 SE at 18 A to 0.24 +/- 0.02 a 42 A (n = 12). Albumin (36 A pradius) behaved in a manner similar to a 40 A dextran, CL/CV averaging 0.33 +/- 0.03 (n = 12) in hydropenia. For all dextran sizes studied and for albumin, CL/CV decreased markedly during either form of volume expansion. Ringer loading produced significantly greater decreases in CL/CV for the larger dextrans and albumin than did plasma loading, and also resulted in much greater increases in renal lymph flow, while causing increases in whole kidney fluid reabsorption similar to those with plasma loading. With a compartmental model in which diffusion of dextrans and albumin from capillary lumen to interstitium is opposed by capillary uptake of tubule reabsorbate, these results are interpreted to indicate that connective reflection coefficients for dextrans with radii great than or equal to 36 A and albumin are essentially equal to 1. Indirect evidence is therefore provided that albumin and the larger globulins exert their full osmotic pressures across the walls of peritubular capillaries.", "PMID": 961878}
{"id": "pubmed23n0032_4722", "title": "Hepatic venous compliance and role of liver as a blood reservoir.", "content": "Changes in hepatic blood volume in response to rapid elevations in hepatic venous pressure were examined in cats using hepatic plethysmography. The liver was intact and received blood from an intact portal vein and hepatic artery. The hepatic blood volume increased in response to elevated venous pressure. Compliance of the hepatic capacitance vessels became greater as the distending venous pressure was increased over the range of venous pressures studied (0-9.4 mmHg). When hepatic venous pressure was elevated to 9.4 MMHg, the hepatic blood volume more than doubled. The liver serves as an important buffer for rapid changes in blood volume, the importance increasing with greater infused volumes of fluid. While overall venous compliance decreased at distending pressures in excess of 5-6 mmHg, the compliance of the hepatic capacitance vessels shows marked increases at pressures above this level. Expansions of the blood volume results in elevations of central venous pressure. Within a few minutes the hepatic capacitance vessels sequester a significant proportion of the added volume. As long as central venous pressure is raised, the liver demonstrates a secondary fluid buffering role by filtering large volumes of fluid across the vascular bed into the peritoneum.", "contents": "Hepatic venous compliance and role of liver as a blood reservoir. Changes in hepatic blood volume in response to rapid elevations in hepatic venous pressure were examined in cats using hepatic plethysmography. The liver was intact and received blood from an intact portal vein and hepatic artery. The hepatic blood volume increased in response to elevated venous pressure. Compliance of the hepatic capacitance vessels became greater as the distending venous pressure was increased over the range of venous pressures studied (0-9.4 mmHg). When hepatic venous pressure was elevated to 9.4 MMHg, the hepatic blood volume more than doubled. The liver serves as an important buffer for rapid changes in blood volume, the importance increasing with greater infused volumes of fluid. While overall venous compliance decreased at distending pressures in excess of 5-6 mmHg, the compliance of the hepatic capacitance vessels shows marked increases at pressures above this level. Expansions of the blood volume results in elevations of central venous pressure. Within a few minutes the hepatic capacitance vessels sequester a significant proportion of the added volume. As long as central venous pressure is raised, the liver demonstrates a secondary fluid buffering role by filtering large volumes of fluid across the vascular bed into the peritoneum.", "PMID": 961879}
{"id": "pubmed23n0032_4723", "title": "Sympathetic nervous control of cat ileocecal sphincter.", "content": "A perfusion method was designed in order to investigate that effects of stimulation of the splanchnic and lumbar colonic nerves on the ileocecal sphincter (ICS) of the cat. Splanchnic and lumbar colonic nerve stimulation at physiological frequencies, i.e., below 10-12 impulses/s, elicited a contraction of the sphincter concomitant with an inhibition of the motility of the adjacent parts of the small and large intestines. It is concluded that the splanchinc and lumbar colonic nerves contract the ICS by a direct effect on the sphincter and not via a contraction of the intestine surrounding the sphincter. Guanethidine and phenoxybenzamine but not propranolol blocked the contraction of the ICS. It is suggested that the splanchnic and lumbar colonic nerves control the sphincter via an alpha-adrenergic mechanism. Inhibitory fibers within the splanchnic or lumbar colonic nerves to the ICS were not found. The excitatory in the ICS elicited by adrenaline, nor-adrenaline, or phyenylephrine infusion was completely blocked by phenoxybenzamine. Propranolol did not block the effect on ICS of these drugs. On the contrary, an augmented response could be shown, indicating unmasking of an excitatory alpha-receptor response by blockade of inhibitory beta-receptors. Further support for these inhibitory beta-receptors was provided by the finding that isoprenaline had a relaxing effect on the sphincteric muscle, an effect that was blocked by propranolol. In order to block the inhibitory effect on the small and large intestine elicited by splanchnic and lumbar colonic nerve stimulation, both phenoxybenzamine and propranolol had to be administered.", "contents": "Sympathetic nervous control of cat ileocecal sphincter. A perfusion method was designed in order to investigate that effects of stimulation of the splanchnic and lumbar colonic nerves on the ileocecal sphincter (ICS) of the cat. Splanchnic and lumbar colonic nerve stimulation at physiological frequencies, i.e., below 10-12 impulses/s, elicited a contraction of the sphincter concomitant with an inhibition of the motility of the adjacent parts of the small and large intestines. It is concluded that the splanchinc and lumbar colonic nerves contract the ICS by a direct effect on the sphincter and not via a contraction of the intestine surrounding the sphincter. Guanethidine and phenoxybenzamine but not propranolol blocked the contraction of the ICS. It is suggested that the splanchnic and lumbar colonic nerves control the sphincter via an alpha-adrenergic mechanism. Inhibitory fibers within the splanchnic or lumbar colonic nerves to the ICS were not found. The excitatory in the ICS elicited by adrenaline, nor-adrenaline, or phyenylephrine infusion was completely blocked by phenoxybenzamine. Propranolol did not block the effect on ICS of these drugs. On the contrary, an augmented response could be shown, indicating unmasking of an excitatory alpha-receptor response by blockade of inhibitory beta-receptors. Further support for these inhibitory beta-receptors was provided by the finding that isoprenaline had a relaxing effect on the sphincteric muscle, an effect that was blocked by propranolol. In order to block the inhibitory effect on the small and large intestine elicited by splanchnic and lumbar colonic nerve stimulation, both phenoxybenzamine and propranolol had to be administered.", "PMID": 961880}
{"id": "pubmed23n0032_4724", "title": "An examination of transcapillary water flux in renal inner medulla.", "content": "We recently demonstrated that net fluid uptake occurs in the capillary system of the inner medulla. To define the site of fluid uptake, the concentration of protein was determined in plasma from descending vasa recta at the base and tip of the exposed papilla in Munich-Wister rats. The vasa recta plasma-to-arterial plasma protein concentration ratio (VR/P) was 1.43 +/- 0.09 at the base and 1.66 +/- 0.09 at the tip. These results, which indicate fluid loss from the descending vasa recta, are difficult to explain on the basic of hydraulic and oncotic forces alone. The osmolality of the contents of descending vasa recta increased between base and tip (delta = 72 +/- 30 mosmol/kg H2O). If the increase in osmolality of plasma in descending vasa recta lags behind that of the adjacent medullary interstitium, a transcapillary osmotic driving force exists favoring water loss from descending vessels. It is concluded that fluid uptake by the inner medullary circulation occurs beyond descending vasa recta in interconnecting capillaries or ascending vasa recta. In our view the most likely interpretation of these results is that fluid movement across vasa recta in the inner medulla is influenced by three forces: those owing to transcapillary differences in osmotic, oncotic, and hydraulic pressures.", "contents": "An examination of transcapillary water flux in renal inner medulla. We recently demonstrated that net fluid uptake occurs in the capillary system of the inner medulla. To define the site of fluid uptake, the concentration of protein was determined in plasma from descending vasa recta at the base and tip of the exposed papilla in Munich-Wister rats. The vasa recta plasma-to-arterial plasma protein concentration ratio (VR/P) was 1.43 +/- 0.09 at the base and 1.66 +/- 0.09 at the tip. These results, which indicate fluid loss from the descending vasa recta, are difficult to explain on the basic of hydraulic and oncotic forces alone. The osmolality of the contents of descending vasa recta increased between base and tip (delta = 72 +/- 30 mosmol/kg H2O). If the increase in osmolality of plasma in descending vasa recta lags behind that of the adjacent medullary interstitium, a transcapillary osmotic driving force exists favoring water loss from descending vessels. It is concluded that fluid uptake by the inner medullary circulation occurs beyond descending vasa recta in interconnecting capillaries or ascending vasa recta. In our view the most likely interpretation of these results is that fluid movement across vasa recta in the inner medulla is influenced by three forces: those owing to transcapillary differences in osmotic, oncotic, and hydraulic pressures.", "PMID": 961881}
{"id": "pubmed23n0032_4725", "title": "Role of sinoatrial ring bundle in internodal conduction.", "content": "The role of the sinoatrial ring bundle (SARB) in internodal conduction was examined by the microelectrode technique in excised rabbit hearts. The spread of the sinus impluse to the surrounding tissues was shown to proceed anteriorly toward the right branch of the crista terminalis significantly faster than toward the other direction. Thus the right SARB and the right branch of the crista terminalis close to the sinus node were the earliest areas excited by the sinus impulse in the areas surrounding the sinus node. It was further shown that the activation sequence does not initiate from the right SARB to the right branch of the crista terminalis via the junction of these two structures. Cutting the SARB did not produce any delay in conduction from the sinus node to the atrioventricular (AV) node. The conduction velocity measured at the endocardial surface by two microelectrodes has proved that conduction in the crista terminalis was significantly faster than in the SARB. The upstroke of the action potential from the crista terminalis was also steeper than that from the SARB. These results suggest that the SARB is not the main route for impulse propagation from the sinus node to the AV node; the fastest internodal conduction therefore takes place with wide wave fronts, along the crista terminalis.", "contents": "Role of sinoatrial ring bundle in internodal conduction. The role of the sinoatrial ring bundle (SARB) in internodal conduction was examined by the microelectrode technique in excised rabbit hearts. The spread of the sinus impluse to the surrounding tissues was shown to proceed anteriorly toward the right branch of the crista terminalis significantly faster than toward the other direction. Thus the right SARB and the right branch of the crista terminalis close to the sinus node were the earliest areas excited by the sinus impulse in the areas surrounding the sinus node. It was further shown that the activation sequence does not initiate from the right SARB to the right branch of the crista terminalis via the junction of these two structures. Cutting the SARB did not produce any delay in conduction from the sinus node to the atrioventricular (AV) node. The conduction velocity measured at the endocardial surface by two microelectrodes has proved that conduction in the crista terminalis was significantly faster than in the SARB. The upstroke of the action potential from the crista terminalis was also steeper than that from the SARB. These results suggest that the SARB is not the main route for impulse propagation from the sinus node to the AV node; the fastest internodal conduction therefore takes place with wide wave fronts, along the crista terminalis.", "PMID": 961882}
{"id": "pubmed23n0032_4726", "title": "Renal excretion of urate in mongrel and Dalmatian dogs: a micropuncture study.", "content": "Free-low micropunction experiments were performed in mongrel dogs and in Dalmatian coach hounds infused with urate to obtain Purate levels of 0.15-0.21 mM before and during the infusion of pyrazinioc acid (PZA). In the absence of PZA, mongrel dogs excreted approximately 50% and Dalmatians 140% of filtered loads of urate. In mongrel dogs net reabsorption occurred only in the proximal convoluted tubules. PZA enhanced net proximal reabsorption and revealed the occurrence of proximal secretion, whereas fractional urate excretion in the urine decreased only slightly. In Dalmation dogs urate fluxes across walls of proximal convoluted tubules resulted in either net reabsorption or net secretion, with no mean change. Net urate secretion occurred between superficial late-proximal and early-distal tubules, and considerably decreased fractional excretion of urate. The renal handling of PZA was similar in mongrel and in Dalmatian dogs.", "contents": "Renal excretion of urate in mongrel and Dalmatian dogs: a micropuncture study. Free-low micropunction experiments were performed in mongrel dogs and in Dalmatian coach hounds infused with urate to obtain Purate levels of 0.15-0.21 mM before and during the infusion of pyrazinioc acid (PZA). In the absence of PZA, mongrel dogs excreted approximately 50% and Dalmatians 140% of filtered loads of urate. In mongrel dogs net reabsorption occurred only in the proximal convoluted tubules. PZA enhanced net proximal reabsorption and revealed the occurrence of proximal secretion, whereas fractional urate excretion in the urine decreased only slightly. In Dalmation dogs urate fluxes across walls of proximal convoluted tubules resulted in either net reabsorption or net secretion, with no mean change. Net urate secretion occurred between superficial late-proximal and early-distal tubules, and considerably decreased fractional excretion of urate. The renal handling of PZA was similar in mongrel and in Dalmatian dogs.", "PMID": 961883}
{"id": "pubmed23n0032_4727", "title": "Left ventricular force-length relations of isovolumic and ejecting contractions.", "content": "To determine the interrelationships between ejecting and isovolumic force-length relations and the extent to which the left ventricle will shorten, data obtained in 27 isolated, servo-regulated hearts were examined. For each heart a series of contractions, variably loaded (delta L) were derived for a thickwalled sphere and normalized by the cross-sectional area of muscle and length at zero end-diastolic pressure. It was found that within the physiological range examined total and active force were essentially a linear function of initial L with respective increments or reductions in slope produced by positive or negative shifts in contractile state. The force-L relations obtained isovolumically and at end ejection were virtually identical. For a given ejection pressure, end-systolic L was constant, despite variations in filling and therefore independent of initial L and deltaL; moreover, the L to which the ventricle shortened was determined by the course of the systolic force L-relation. Thus, irrespective of loading, delta L occurs within the confines of the contractile state-dependent isovolumic force-L relation and where the latter is equivalent to the end-systolic force-length relation.", "contents": "Left ventricular force-length relations of isovolumic and ejecting contractions. To determine the interrelationships between ejecting and isovolumic force-length relations and the extent to which the left ventricle will shorten, data obtained in 27 isolated, servo-regulated hearts were examined. For each heart a series of contractions, variably loaded (delta L) were derived for a thickwalled sphere and normalized by the cross-sectional area of muscle and length at zero end-diastolic pressure. It was found that within the physiological range examined total and active force were essentially a linear function of initial L with respective increments or reductions in slope produced by positive or negative shifts in contractile state. The force-L relations obtained isovolumically and at end ejection were virtually identical. For a given ejection pressure, end-systolic L was constant, despite variations in filling and therefore independent of initial L and deltaL; moreover, the L to which the ventricle shortened was determined by the course of the systolic force L-relation. Thus, irrespective of loading, delta L occurs within the confines of the contractile state-dependent isovolumic force-L relation and where the latter is equivalent to the end-systolic force-length relation.", "PMID": 961884}
{"id": "pubmed23n0032_4728", "title": "Fibrin-induced release of platelet serotonin.", "content": "Human platelets were reacted with polymerized fibrin formed from human fibrinogen. The platelets adhered to the fibrin particles and this adhesion was followed by the release of serotonin from prelabeled platelets. The adhesion of platelets to fibrin was not inhibited by adenosine or prostaglandin E1. However, the subsequent Ca2+-dependent release of platelet serotonin was completely inhibited by these compounds. After the initial platelet-fibrin interaction, ADP and serotonin released from activated platelets may lead to additional platelet aggregation and release. Therefore, in addition to clot stabilization, fibrin serves as an initiator of the platelet release reaction. This in turn initiates the self-amplifying process of platelet aggregation.", "contents": "Fibrin-induced release of platelet serotonin. Human platelets were reacted with polymerized fibrin formed from human fibrinogen. The platelets adhered to the fibrin particles and this adhesion was followed by the release of serotonin from prelabeled platelets. The adhesion of platelets to fibrin was not inhibited by adenosine or prostaglandin E1. However, the subsequent Ca2+-dependent release of platelet serotonin was completely inhibited by these compounds. After the initial platelet-fibrin interaction, ADP and serotonin released from activated platelets may lead to additional platelet aggregation and release. Therefore, in addition to clot stabilization, fibrin serves as an initiator of the platelet release reaction. This in turn initiates the self-amplifying process of platelet aggregation.", "PMID": 961885}
{"id": "pubmed23n0032_4729", "title": "Lactate production in isolated perfused rat lung.", "content": "The ability of the isolated perfused rat lung to produce lactate under aerobic and hypoxic conditions has been studied. The lung has been found capable of producing lactate at a rate of 53 mumol/g dry wt per h in the presence of as great as 1 mM extracellular lactate under aerobic conditions. Essentially 100% of the lactate synthesized was derived from [U-14C]glucose. When hypoxic, the rate of lactate production doubled, but only 60% was found to be derived from [U-14C]glucose. When the perfused lung was exposed to aerobic conditions following hypoxia, it was found that lactate production did not decrease. However, lactate production from [U-14C]glucose did increase. It is concluded that lung tissue possesses a high rate of lactate production under aerobic contitions from glucose in comparison to glucose oxidation to CO2. When the lung is hypoxic, lactate production increases as a probable result of amino acid catabolism with little anaerobic energy production occurring. The effect of hypoxia was not reversed within the duration of the performed experiments.", "contents": "Lactate production in isolated perfused rat lung. The ability of the isolated perfused rat lung to produce lactate under aerobic and hypoxic conditions has been studied. The lung has been found capable of producing lactate at a rate of 53 mumol/g dry wt per h in the presence of as great as 1 mM extracellular lactate under aerobic conditions. Essentially 100% of the lactate synthesized was derived from [U-14C]glucose. When hypoxic, the rate of lactate production doubled, but only 60% was found to be derived from [U-14C]glucose. When the perfused lung was exposed to aerobic conditions following hypoxia, it was found that lactate production did not decrease. However, lactate production from [U-14C]glucose did increase. It is concluded that lung tissue possesses a high rate of lactate production under aerobic contitions from glucose in comparison to glucose oxidation to CO2. When the lung is hypoxic, lactate production increases as a probable result of amino acid catabolism with little anaerobic energy production occurring. The effect of hypoxia was not reversed within the duration of the performed experiments.", "PMID": 961886}
{"id": "pubmed23n0032_4730", "title": "Glucagon, insulin, and gluconeogenesis in fasted odd carbon fatty acid-enriched rats.", "content": "Forty-eight male rats were fed a nutritionally complete diet containing 30% of dietary energy as fat. For 24 animals (control) the fat source was corn oil, for the remaining 24 rats (experimental) the fat source was a triundecanoin-corn oil mixture (7:3, wt/wt). After 6 wk, groups of control and experimental rats were killed after 0, 24, and 48 h of fasting. In the experimental group, adipose tissue fatty acids contained, on average, 280 mmol undecanoate/mol fatty acid. In the control group, no odd-numbered fatty acids were present. During fasting, the experimental groups had higher plasma glucose and alanine levels, higher plasma insulin-to-glucagon ration, and lower liver phosphenol pyruvate caboxykinase. The results suggest that the terminal propionate residues generated when odd carbon fatty acids are oxidized become gluconeogenic precursors and cause a reduced need for gluconeogenesis from protein.", "contents": "Glucagon, insulin, and gluconeogenesis in fasted odd carbon fatty acid-enriched rats. Forty-eight male rats were fed a nutritionally complete diet containing 30% of dietary energy as fat. For 24 animals (control) the fat source was corn oil, for the remaining 24 rats (experimental) the fat source was a triundecanoin-corn oil mixture (7:3, wt/wt). After 6 wk, groups of control and experimental rats were killed after 0, 24, and 48 h of fasting. In the experimental group, adipose tissue fatty acids contained, on average, 280 mmol undecanoate/mol fatty acid. In the control group, no odd-numbered fatty acids were present. During fasting, the experimental groups had higher plasma glucose and alanine levels, higher plasma insulin-to-glucagon ration, and lower liver phosphenol pyruvate caboxykinase. The results suggest that the terminal propionate residues generated when odd carbon fatty acids are oxidized become gluconeogenic precursors and cause a reduced need for gluconeogenesis from protein.", "PMID": 961887}
{"id": "pubmed23n0032_4731", "title": "Renal urate excretion at various plasma concentrations in the rat: a free-flow micropuncture study.", "content": "Free-flow micropuncture experiments were performed in male Sprague-Dawley rats undergoing moderate mannitol diuresis and infused with urate-containing solutions. The resulting plasma urate concentrations ranged from 37.5 +/- 2.4 to 601.2 +/- 23.8 muM. With urate loading, the fraction of filtered urate excreted in pelvic urine increased from 0.32 +/- 0.02 to 0.92 +/- 0.05 mu M, but net secretion was not observed. At normal urate levels net reabsorption occurred along superficial proximal tubules, whereas net secretion could be demonstrated at the highest plasma urate levels. Net movements of urate did not appear to occur across the walls of the lower segments of nephrons.", "contents": "Renal urate excretion at various plasma concentrations in the rat: a free-flow micropuncture study. Free-flow micropuncture experiments were performed in male Sprague-Dawley rats undergoing moderate mannitol diuresis and infused with urate-containing solutions. The resulting plasma urate concentrations ranged from 37.5 +/- 2.4 to 601.2 +/- 23.8 muM. With urate loading, the fraction of filtered urate excreted in pelvic urine increased from 0.32 +/- 0.02 to 0.92 +/- 0.05 mu M, but net secretion was not observed. At normal urate levels net reabsorption occurred along superficial proximal tubules, whereas net secretion could be demonstrated at the highest plasma urate levels. Net movements of urate did not appear to occur across the walls of the lower segments of nephrons.", "PMID": 961888}
{"id": "pubmed23n0032_4732", "title": "Time course of ureteral changes with obstruction.", "content": "Chronic obstruction is characterized by a marked degree of ureteral dilatation and tortuosity which develops in the presence of a relatively low intraluminal pressure. To define the mechanism for the development of this relationship,  the pressure-tension-deformation relationships of acute and chronically obstructed ureters were investigated. Rabbit ureters, in vivo, were obstructed and deformation correlated with intraluminal pressure. During the initial 3 h of obstruction, ureteral resting pressure increased from approximately 0 to 42 cm H2O, and diameter increased 16% with a slight increase in length. Subsequently, average wall tension (tensile stress) and intraluminal pressure declined, although deformation persisted. Between 6 h and 8 days after the onset of obstruction, intraluminal pressure remained essentially unchanged. During this time interval, ureteral deformation continued gradually and progressively as the ureter underwent creep both circumferentially and longitudinally. This was associated with a marked increase in average wall tension. At 8 days the diameter had increased by 170% and the length by 25%.", "contents": "Time course of ureteral changes with obstruction. Chronic obstruction is characterized by a marked degree of ureteral dilatation and tortuosity which develops in the presence of a relatively low intraluminal pressure. To define the mechanism for the development of this relationship,  the pressure-tension-deformation relationships of acute and chronically obstructed ureters were investigated. Rabbit ureters, in vivo, were obstructed and deformation correlated with intraluminal pressure. During the initial 3 h of obstruction, ureteral resting pressure increased from approximately 0 to 42 cm H2O, and diameter increased 16% with a slight increase in length. Subsequently, average wall tension (tensile stress) and intraluminal pressure declined, although deformation persisted. Between 6 h and 8 days after the onset of obstruction, intraluminal pressure remained essentially unchanged. During this time interval, ureteral deformation continued gradually and progressively as the ureter underwent creep both circumferentially and longitudinally. This was associated with a marked increase in average wall tension. At 8 days the diameter had increased by 170% and the length by 25%.", "PMID": 961889}
{"id": "pubmed23n0032_4733", "title": "Biliary excretion of dye in dogs infused with BSP or its glutathione conjugate.", "content": "A comparison of the maximal rates of biliary excretion (Tm), of dye in dogs infused with either BSP or its glutathione conjugate (BSP-GSH) was carried out. Tm was much higher when BSP-GSH rather than BSP was infused. This was accounted for by a significantly higher concentration of dye in bile of dogs receiving BSP-GSH. Evidence is presented that BSP and its conjugated metabolites compete for a common transport carrier and that BSP disproportionately depresses the biliary excretion of conjugated dye compounds. This latter observation accounts for the depressed dye Tm found during infusion of BSP. Choleresis invariably accompanied dye excretion. When BSP-GSH was infused, enhanced bile flow could be accounted for by the predicted osmotic activity of dye transported into bile. By contrast, the choleresis measured during infusion of BSP was significantly greater than that predicted. An additional mechanism for choleresis is operative, therefore, when unconjugated BSP is infused. Administration of taurocholate enhanced dye Tm when BSP-GSH was infused. Since increments of canalicular bile flow induced by theophylline and glucagon did not enhance dye excretion into bile, this effect by taurocholate appears to be related to taurocholate excretion per se rather than to the enhanced canalicular bile flow which accompanies its excretion.", "contents": "Biliary excretion of dye in dogs infused with BSP or its glutathione conjugate. A comparison of the maximal rates of biliary excretion (Tm), of dye in dogs infused with either BSP or its glutathione conjugate (BSP-GSH) was carried out. Tm was much higher when BSP-GSH rather than BSP was infused. This was accounted for by a significantly higher concentration of dye in bile of dogs receiving BSP-GSH. Evidence is presented that BSP and its conjugated metabolites compete for a common transport carrier and that BSP disproportionately depresses the biliary excretion of conjugated dye compounds. This latter observation accounts for the depressed dye Tm found during infusion of BSP. Choleresis invariably accompanied dye excretion. When BSP-GSH was infused, enhanced bile flow could be accounted for by the predicted osmotic activity of dye transported into bile. By contrast, the choleresis measured during infusion of BSP was significantly greater than that predicted. An additional mechanism for choleresis is operative, therefore, when unconjugated BSP is infused. Administration of taurocholate enhanced dye Tm when BSP-GSH was infused. Since increments of canalicular bile flow induced by theophylline and glucagon did not enhance dye excretion into bile, this effect by taurocholate appears to be related to taurocholate excretion per se rather than to the enhanced canalicular bile flow which accompanies its excretion.", "PMID": 961890}
{"id": "pubmed23n0032_4734", "title": "Vitamin K2 absorption by rat everted small intestinal sacs.", "content": "Small intestinal absorption of vitamin K2 was investigated in vitro. Experiments with increasing concentrations of the vitamin up to 900 nM revealed linerity between the concentration and the rate of absorption (r = 0.99). Addition of metabolic uncouplers and inhibitors such as 2,4-dinitrophenol, sodium azide, and potassium cyanide did not decrease the rate of absorption of the vitamin (P less than 0.05). Absorption rate of the vitamin increased when taurocholate was replaced by a nonionic detergent, Pluronic F-68. The addition of butyric and octanoic acids to the incubation solution caused an increase in the absorption rate of vitamin K2. No change in the absorption of the vitamin occurred in the presence of oleic and linoleic acid. Addition to vitamins K1 and K3 to the incubation solution did not change the rate of vitamin K2 absorption. These findings suggest that vitamin K2 is absorbed by the small bowel by a passive noncarrier-mediated diffusion process. The rate of diffusion varied when the lipid and bile salt composition of the incubation solution was modified. Distal intestinal absorption of vitamin K from bacterial sources coupled with colonic absorption of the vitamin may be the major constant source of vitamin K in mammals.", "contents": "Vitamin K2 absorption by rat everted small intestinal sacs. Small intestinal absorption of vitamin K2 was investigated in vitro. Experiments with increasing concentrations of the vitamin up to 900 nM revealed linerity between the concentration and the rate of absorption (r = 0.99). Addition of metabolic uncouplers and inhibitors such as 2,4-dinitrophenol, sodium azide, and potassium cyanide did not decrease the rate of absorption of the vitamin (P less than 0.05). Absorption rate of the vitamin increased when taurocholate was replaced by a nonionic detergent, Pluronic F-68. The addition of butyric and octanoic acids to the incubation solution caused an increase in the absorption rate of vitamin K2. No change in the absorption of the vitamin occurred in the presence of oleic and linoleic acid. Addition to vitamins K1 and K3 to the incubation solution did not change the rate of vitamin K2 absorption. These findings suggest that vitamin K2 is absorbed by the small bowel by a passive noncarrier-mediated diffusion process. The rate of diffusion varied when the lipid and bile salt composition of the incubation solution was modified. Distal intestinal absorption of vitamin K from bacterial sources coupled with colonic absorption of the vitamin may be the major constant source of vitamin K in mammals.", "PMID": 961891}
{"id": "pubmed23n0032_4735", "title": "Effects of norepinephrine on mechanics of arteries in vitro.", "content": "The effects of smooth muscle activation on the pressure dependence of arterial wall characteristic impedance were studied with isolated segments of canine iliac and carotid arteries. Measurements of external diameter and transmural pressure were made before and after activation of the arterial smooth muscle (SM) by norepinephrine (NE) in concentrations of 0.5 and 5 mug/ml and used to compute values of characteristic impedance (Z0). In the absence of SM tone, values of Z0 for both arterial sites increased monotonically with transmural pressure. For the larger [NE], values of ZO exhibited a minimum at pressures of the order of 125 mmHg and increased for both larger and smaller values of pressure, For the smaller [NE], values of Z0 showed a similar pressure dependence but with a broader minimum. It is concluded that the previously demonstrated constancy of vascular impedance with changes in arterial pressure is at least the result of the presence of a tonic level of SM activation in conduit arteries.", "contents": "Effects of norepinephrine on mechanics of arteries in vitro. The effects of smooth muscle activation on the pressure dependence of arterial wall characteristic impedance were studied with isolated segments of canine iliac and carotid arteries. Measurements of external diameter and transmural pressure were made before and after activation of the arterial smooth muscle (SM) by norepinephrine (NE) in concentrations of 0.5 and 5 mug/ml and used to compute values of characteristic impedance (Z0). In the absence of SM tone, values of Z0 for both arterial sites increased monotonically with transmural pressure. For the larger [NE], values of ZO exhibited a minimum at pressures of the order of 125 mmHg and increased for both larger and smaller values of pressure, For the smaller [NE], values of Z0 showed a similar pressure dependence but with a broader minimum. It is concluded that the previously demonstrated constancy of vascular impedance with changes in arterial pressure is at least the result of the presence of a tonic level of SM activation in conduit arteries.", "PMID": 961892}
{"id": "pubmed23n0032_4736", "title": "Degradation of prostaglandin E2 and F2alpha by the canine liver.", "content": "Degradation of prostaglandin E2 (PGE2) and F2 alpha (PGF2 alpha) by the liver was determined in 16 dogs. PGE2 (8 dogs) or PGF2 alpha (8 dogs) was infused into the left ventricle at rates of 3.75, 7.5, 15, 37.5, and 75 mug/min. Blood samples obtained simultaneously from the portal vein (PV), hepatic vein (HV), and abdominal inferior vena cava (IVC) at each infusion rate were tested for PGE or PGF concentration by radioimmunoassy. During PGE2 infusion the mean PGE concentrations in the PV were 0.60, 1.05, 1.40, 2.29, 4.80, and 7.29 ng/ml at each infusion rate, Corresponding concentrations in the HV were 0.51, 0.62, 0.71, 0.96, 1.94, and 2.62 ng/ml, and in the IVC 0.51, 0.95, 1.31, 1.96, 4.31, AND 5;18 NG/ML. During PGF2 alpha infusion, the mean PGF concentrations in the PV were 0.32, 0.59, 0.73, 1.73, 4.11, and 7.11 ng/ml at the respective infusion rates; Corresponding concentrations in the HV were 0.26, 0.24, 0.33, 0.48, 0.49, and 0.96 ng/ml, and in the IVC 0.32, 0.50, 0.61, 1.10, 2.40, and 4.17 ng/ml. Thus, these data indicate that the canine liver has substantial, but not unlimited, capacity for degradation of PGE2 and PGF2 alpha in the portal circulation. Whether or not PGE or PGF levels in the PV may exceed this capacity during periods of stress or whether this enzymatic mechanism may be suppressed by either physiological or pharmacological factors is not known.", "contents": "Degradation of prostaglandin E2 and F2alpha by the canine liver. Degradation of prostaglandin E2 (PGE2) and F2 alpha (PGF2 alpha) by the liver was determined in 16 dogs. PGE2 (8 dogs) or PGF2 alpha (8 dogs) was infused into the left ventricle at rates of 3.75, 7.5, 15, 37.5, and 75 mug/min. Blood samples obtained simultaneously from the portal vein (PV), hepatic vein (HV), and abdominal inferior vena cava (IVC) at each infusion rate were tested for PGE or PGF concentration by radioimmunoassy. During PGE2 infusion the mean PGE concentrations in the PV were 0.60, 1.05, 1.40, 2.29, 4.80, and 7.29 ng/ml at each infusion rate, Corresponding concentrations in the HV were 0.51, 0.62, 0.71, 0.96, 1.94, and 2.62 ng/ml, and in the IVC 0.51, 0.95, 1.31, 1.96, 4.31, AND 5;18 NG/ML. During PGF2 alpha infusion, the mean PGF concentrations in the PV were 0.32, 0.59, 0.73, 1.73, 4.11, and 7.11 ng/ml at the respective infusion rates; Corresponding concentrations in the HV were 0.26, 0.24, 0.33, 0.48, 0.49, and 0.96 ng/ml, and in the IVC 0.32, 0.50, 0.61, 1.10, 2.40, and 4.17 ng/ml. Thus, these data indicate that the canine liver has substantial, but not unlimited, capacity for degradation of PGE2 and PGF2 alpha in the portal circulation. Whether or not PGE or PGF levels in the PV may exceed this capacity during periods of stress or whether this enzymatic mechanism may be suppressed by either physiological or pharmacological factors is not known.", "PMID": 961893}
{"id": "pubmed23n0032_4737", "title": "Responses of abdominal vascular capacitance to stimulation of splachnic nerves.", "content": "In chloralose-anesthetized dogs the abdominal circulation was vascularly isolated without opening the abdominal cavity. The region was perfused at constant flow through the aorta and drained at constant pressure from the inferior vena cava. Changes in resistance were calculated from changes in perfusion pressure and changes in capacitance were calculated by integrating changes in venous outflow. Stimulation of both splanchnic nerves at 20 Hz increased resistance by 135% and reduced capacitance by 7.20 ml kg-1. The capacitance responses at 1 and 2 Hz (3.42 and 5.43 ml kg-1) were 48 and 67% of the responses at 20 Hz, However, the resistance responses at 1 and 2 Hz (14 and 31% increase) were only 12 and 26% of the responses at 20 Hz. After occlusion of the splenic pedicle, capacitance responses were reduced by about 40%. Although changes in inferior vena caval pressure changed the volume of blood in the abdomen by 0.92 ml kg-1 cmH2O-1, the responses to stimulation were relatively constant in any one animal at constant venous pressures between 5 and 15 cmH2O.", "contents": "Responses of abdominal vascular capacitance to stimulation of splachnic nerves. In chloralose-anesthetized dogs the abdominal circulation was vascularly isolated without opening the abdominal cavity. The region was perfused at constant flow through the aorta and drained at constant pressure from the inferior vena cava. Changes in resistance were calculated from changes in perfusion pressure and changes in capacitance were calculated by integrating changes in venous outflow. Stimulation of both splanchnic nerves at 20 Hz increased resistance by 135% and reduced capacitance by 7.20 ml kg-1. The capacitance responses at 1 and 2 Hz (3.42 and 5.43 ml kg-1) were 48 and 67% of the responses at 20 Hz, However, the resistance responses at 1 and 2 Hz (14 and 31% increase) were only 12 and 26% of the responses at 20 Hz. After occlusion of the splenic pedicle, capacitance responses were reduced by about 40%. Although changes in inferior vena caval pressure changed the volume of blood in the abdomen by 0.92 ml kg-1 cmH2O-1, the responses to stimulation were relatively constant in any one animal at constant venous pressures between 5 and 15 cmH2O.", "PMID": 961894}
{"id": "pubmed23n0032_4738", "title": "Effects of food deprivation on protein synthesis and degradation in rat skeletal muscles.", "content": "The effects of food deprivation on protein turnover in rat soleus and extensor digitorum longus (EDL) were investigated. Muscles were removed from fed or fasted growing rats, and protein synthesis and breakdown were measured during incubation in vitro. Rates of synthesis and degradation were higher in the dark soleus than in the pale EDL. One day after food removal protein synthesis and RNA content in the EDL decreased. On the 2nd day of fasting, rates of protein catabolism in this muscle increased. Little or no change in synthesis and degradation occurred in the soleus. Consequently, during fasting the soleus lost much less weight than the EDL and other rat muscles. In unsupplemented buffer or in medium containing amino acids, glucose, and insulin, the muscles of fasted rats showed a lower rate of protein synthesis expressed per milligram of tissue but not per microgram of RNA. Thus the decrease in muscle RNA on fasting was responsible for the reduced synthesis observed under controlled in vitro conditions. In vivo the reduction in muscle protein synthesis on fasting results both from a lower RNA content and lower rate of synthesis per microgram of RNA. Reduced supply of glucose, insulin, and amino acids may account for the lower rate of synthesis per microgram of RNA demonstrable in vivo.", "contents": "Effects of food deprivation on protein synthesis and degradation in rat skeletal muscles. The effects of food deprivation on protein turnover in rat soleus and extensor digitorum longus (EDL) were investigated. Muscles were removed from fed or fasted growing rats, and protein synthesis and breakdown were measured during incubation in vitro. Rates of synthesis and degradation were higher in the dark soleus than in the pale EDL. One day after food removal protein synthesis and RNA content in the EDL decreased. On the 2nd day of fasting, rates of protein catabolism in this muscle increased. Little or no change in synthesis and degradation occurred in the soleus. Consequently, during fasting the soleus lost much less weight than the EDL and other rat muscles. In unsupplemented buffer or in medium containing amino acids, glucose, and insulin, the muscles of fasted rats showed a lower rate of protein synthesis expressed per milligram of tissue but not per microgram of RNA. Thus the decrease in muscle RNA on fasting was responsible for the reduced synthesis observed under controlled in vitro conditions. In vivo the reduction in muscle protein synthesis on fasting results both from a lower RNA content and lower rate of synthesis per microgram of RNA. Reduced supply of glucose, insulin, and amino acids may account for the lower rate of synthesis per microgram of RNA demonstrable in vivo.", "PMID": 961895}
{"id": "pubmed23n0032_4739", "title": "Short-range stiffness of slow fibers and twitch fibers in reptilian muscle.", "content": "The semitendinous muscle of the lizard Tilique contains both slow and twitch fibers; by subdivision of its motor nerve, fibers of each type may be stimulated separately. When, during repetitive stimulation of nerve filaments, the muscle was lengthened or shortened, the tension changes included an initial short-range stiffness, followed by a later compliance. With increasing velocities of movement, the short-range stiffness increased toward a limiting value. For slow fibers this limiting value was reached with lower velocities of movement than for the twitch fibers. Provided that the same velocity of movement was used and the movements began from similar initial isometric tensions, the slow fibers resisted the movements with a greater stiffness than the twitch fibers. It is suggested that not all of the observed differences between the two fiber types can be interpreted simply in terms of differences in rates of formation and breakdown of cross-links.", "contents": "Short-range stiffness of slow fibers and twitch fibers in reptilian muscle. The semitendinous muscle of the lizard Tilique contains both slow and twitch fibers; by subdivision of its motor nerve, fibers of each type may be stimulated separately. When, during repetitive stimulation of nerve filaments, the muscle was lengthened or shortened, the tension changes included an initial short-range stiffness, followed by a later compliance. With increasing velocities of movement, the short-range stiffness increased toward a limiting value. For slow fibers this limiting value was reached with lower velocities of movement than for the twitch fibers. Provided that the same velocity of movement was used and the movements began from similar initial isometric tensions, the slow fibers resisted the movements with a greater stiffness than the twitch fibers. It is suggested that not all of the observed differences between the two fiber types can be interpreted simply in terms of differences in rates of formation and breakdown of cross-links.", "PMID": 961896}
{"id": "pubmed23n0032_4740", "title": "Integration of the cardiovagal mechanism in the medulla oblongata of the cat.", "content": "The central cardiovagal mechanism of the medulla oblongata was explored by stimulation and ablation techniques in the anesthetized cat. Insertion of an electrode into the nucleus solitarius (NS) occassionally evoked slight and transient bradycardia, but similar mechanical irritation to the nucleus ambiguus (NA) usually evoked prolonged and intense bradycardia. Electrical stimulation of the dorsal motor nucleus of the vagus (DNV) produced no or little bradycardia. Stimulation of NS and NA consistently produced cardiac slowing with a latency of less than 2 s and the effect was more prominent in the NA. Contralateral vagotomy did not significantly affect the bradycardia on the NS and NA stimulation but ipsilateral vagotomy caused a complete abolition. Lesions of the NA or DNV largely or completely abolished the bradycardia consequent to NS stimulation. Extensive destruction of the NS and/or DNV did not affect the bradycardia resulting from NA stimulation. Destruction of the ventral midline area partially reduced th bradycardia on NS STIMULATION BY 36-54%. The results suggest that the sequence of the three vagal nuclei for cardiac inhibition runs in the following order: NS, DNV, and NA. Synaptic connections are probably scanty in the DNV. Part of the vagal pathway passes through the ventral midline area before it reaches the NA. A scheme of the neural pathway for reflex bradycardia of vagal origin has been proposed.", "contents": "Integration of the cardiovagal mechanism in the medulla oblongata of the cat. The central cardiovagal mechanism of the medulla oblongata was explored by stimulation and ablation techniques in the anesthetized cat. Insertion of an electrode into the nucleus solitarius (NS) occassionally evoked slight and transient bradycardia, but similar mechanical irritation to the nucleus ambiguus (NA) usually evoked prolonged and intense bradycardia. Electrical stimulation of the dorsal motor nucleus of the vagus (DNV) produced no or little bradycardia. Stimulation of NS and NA consistently produced cardiac slowing with a latency of less than 2 s and the effect was more prominent in the NA. Contralateral vagotomy did not significantly affect the bradycardia on the NS and NA stimulation but ipsilateral vagotomy caused a complete abolition. Lesions of the NA or DNV largely or completely abolished the bradycardia consequent to NS stimulation. Extensive destruction of the NS and/or DNV did not affect the bradycardia resulting from NA stimulation. Destruction of the ventral midline area partially reduced th bradycardia on NS STIMULATION BY 36-54%. The results suggest that the sequence of the three vagal nuclei for cardiac inhibition runs in the following order: NS, DNV, and NA. Synaptic connections are probably scanty in the DNV. Part of the vagal pathway passes through the ventral midline area before it reaches the NA. A scheme of the neural pathway for reflex bradycardia of vagal origin has been proposed.", "PMID": 961897}
{"id": "pubmed23n0032_4741", "title": "Gastrointestinal metabolism of cadmium in experimental iron deficiency;.", "content": "The average gastrointestinal uptake 4 h after an intragastric dose of 400 nmol of cadmium chloride labeled with 109CdCl2 in iron-deficient mice, 25%, was significantly greater than the result, 16%, in iron-normal animals, and more cadmium entered the body of the former, 3.8%, than the latter, 2% (P less than 0.05). Between 4 and 72 h, gastrointestinal radioactivity declined without further increase in body activity; however, more radiocadmium remained in the duodenum of iron-deficient than iron-normal animals (P less than 0.05). The radiocadmium sequestered in the duodenum was bound to a protein with a molecular weight of about 12,000. After subcutaneous injection of radiocadmium, the rate of excretion of radioactivity from the body was similar in iron-normal and iron-deficient mice; however, a greater proportion of the injected dose accumulated in the duodenum of the iron-deficient animals (P less than 0.05). Thus, the intestinal adapative response to iron deficiency may enhance cadmium toxicity, whereas sequestration and subsequent excretion of cadmium by the intestinal mucosa serves to protect the body against toxic effects. The duodenum, particularly in iron-deficient mice, is especially vulnerable to the toxic effects of cadmium.", "contents": "Gastrointestinal metabolism of cadmium in experimental iron deficiency;. The average gastrointestinal uptake 4 h after an intragastric dose of 400 nmol of cadmium chloride labeled with 109CdCl2 in iron-deficient mice, 25%, was significantly greater than the result, 16%, in iron-normal animals, and more cadmium entered the body of the former, 3.8%, than the latter, 2% (P less than 0.05). Between 4 and 72 h, gastrointestinal radioactivity declined without further increase in body activity; however, more radiocadmium remained in the duodenum of iron-deficient than iron-normal animals (P less than 0.05). The radiocadmium sequestered in the duodenum was bound to a protein with a molecular weight of about 12,000. After subcutaneous injection of radiocadmium, the rate of excretion of radioactivity from the body was similar in iron-normal and iron-deficient mice; however, a greater proportion of the injected dose accumulated in the duodenum of the iron-deficient animals (P less than 0.05). Thus, the intestinal adapative response to iron deficiency may enhance cadmium toxicity, whereas sequestration and subsequent excretion of cadmium by the intestinal mucosa serves to protect the body against toxic effects. The duodenum, particularly in iron-deficient mice, is especially vulnerable to the toxic effects of cadmium.", "PMID": 961898}
{"id": "pubmed23n0032_4742", "title": "Micropuncture studies of glucose transport in the dog: mechanism of renal glycosuria.", "content": "Clearance and micropuncture studies were performed in 23 dogs without glucose loading to examine the tubule mechanism of renal glycosuria. Studies were carried out in three groups of animals before and after 10% extracellular volume expansion, and administration of maleic acid in low dose at 150 mumol/kg and in high dose at 300 mumol/kg. Specific hexokinase methods were used for the determination of glucose in tubule fluid and urine. Under control conditios, glucose reabsorption occurred predominantly in the proximal tubule. In all three groups, proximal tubule reabsorption of both sodium and glucose was inhibited in the second phase, showing a good correlation between the two. In contrast, fractional urinary glucose excretion remained unchanged after volume expansion and low-dose maleic acid, indicating reabsorption of virtually all the increased glucose load at a further \"distal\" site. On the other hand, significant glycosuria developed after high-dose maleic acid that was a result of reduced glucose reabsorption in the distal nephron, in addition to the proximal effect. It was concluded that distal glucose transport plays a significant role in regulating urinary glucose excretion and maintains renal thershold for glucose,", "contents": "Micropuncture studies of glucose transport in the dog: mechanism of renal glycosuria. Clearance and micropuncture studies were performed in 23 dogs without glucose loading to examine the tubule mechanism of renal glycosuria. Studies were carried out in three groups of animals before and after 10% extracellular volume expansion, and administration of maleic acid in low dose at 150 mumol/kg and in high dose at 300 mumol/kg. Specific hexokinase methods were used for the determination of glucose in tubule fluid and urine. Under control conditios, glucose reabsorption occurred predominantly in the proximal tubule. In all three groups, proximal tubule reabsorption of both sodium and glucose was inhibited in the second phase, showing a good correlation between the two. In contrast, fractional urinary glucose excretion remained unchanged after volume expansion and low-dose maleic acid, indicating reabsorption of virtually all the increased glucose load at a further \"distal\" site. On the other hand, significant glycosuria developed after high-dose maleic acid that was a result of reduced glucose reabsorption in the distal nephron, in addition to the proximal effect. It was concluded that distal glucose transport plays a significant role in regulating urinary glucose excretion and maintains renal thershold for glucose,", "PMID": 961899}
{"id": "pubmed23n0032_4743", "title": "Protein metabolism in burned rats.", "content": "Incorporation of [2-14C]glycine was used to estimate serum protein synthesis in four groups of rats. These were the control (group C); 20% body surface burn (group B); 20% burn, seeded with Pseudomonas aeruginosa (group BI); and burned-infected treated topically with mafenide (alpha-amino-p-toluenesulfonamide) acetate (group BIS), a treatment which controls P, aeruginosa burn-wound infection in humans. On the 6th day postburn the relative specific activities of all fractions were increased in the order BI greater than BIS greater than B greater than C, as were the concentrations of the globulins; Serum albumin concentration fell, being lowest in BI. Tissue albumin contents, measured by radioimmunoassay, of eviscerated blood-free bodies of rats were (mg/100 g rat wt): C, 207; B, 294; BI, 256. Analyses of individual tissues showed that the difference was due to increased albumin content in the burn-wound area. The tissue albumin was of normal molecular size and was immunologically reactive. We conclude that the prolonged hypoalbuminemia following burn injury is not a consequence of impaired albumin synthesis, but a result of altered compartmentation.", "contents": "Protein metabolism in burned rats. Incorporation of [2-14C]glycine was used to estimate serum protein synthesis in four groups of rats. These were the control (group C); 20% body surface burn (group B); 20% burn, seeded with Pseudomonas aeruginosa (group BI); and burned-infected treated topically with mafenide (alpha-amino-p-toluenesulfonamide) acetate (group BIS), a treatment which controls P, aeruginosa burn-wound infection in humans. On the 6th day postburn the relative specific activities of all fractions were increased in the order BI greater than BIS greater than B greater than C, as were the concentrations of the globulins; Serum albumin concentration fell, being lowest in BI. Tissue albumin contents, measured by radioimmunoassay, of eviscerated blood-free bodies of rats were (mg/100 g rat wt): C, 207; B, 294; BI, 256. Analyses of individual tissues showed that the difference was due to increased albumin content in the burn-wound area. The tissue albumin was of normal molecular size and was immunologically reactive. We conclude that the prolonged hypoalbuminemia following burn injury is not a consequence of impaired albumin synthesis, but a result of altered compartmentation.", "PMID": 961900}
{"id": "pubmed23n0032_4744", "title": "Cerebral circulation and norepinephrine: relevance of the blood-brain barrier.", "content": "The systemic administration of norepinephrine has minimal effects on the cerebral circulation, perhaps due to blood-brain barrier mechanisms. To test hypothesis, the cerebrovascular effects of norepinephrine beyond the blood-brain barrier were studied in anesthetized baboons, Intraventricular norepinephrine (40 mug/kg) resulted in significant increases in cerebral blood flow (40%), cerebral oxygen consumption (21%), and cerebral glucose uptake (153%). Intracarotid hypertonic urea opens the blood-brain barrier by osmotic disruption; Consequent to hypertonic urea, the intracarotid infusion of norepinephrine, 50 ng/kg-min, significantly increase cerebral blood flow (49%), cerebral oxygen consumption (21%), and cerebral glucose uptake (76%), It appears probable that the cerebrovascular responses to norepinephrine are dependent on the integrity of the blood-brain barrier; It is likely that the increase in cerebral blood flow, associated with norepinephrine when it bypasses the barrier, is secondary to an increase in cerebral metabolism.", "contents": "Cerebral circulation and norepinephrine: relevance of the blood-brain barrier. The systemic administration of norepinephrine has minimal effects on the cerebral circulation, perhaps due to blood-brain barrier mechanisms. To test hypothesis, the cerebrovascular effects of norepinephrine beyond the blood-brain barrier were studied in anesthetized baboons, Intraventricular norepinephrine (40 mug/kg) resulted in significant increases in cerebral blood flow (40%), cerebral oxygen consumption (21%), and cerebral glucose uptake (153%). Intracarotid hypertonic urea opens the blood-brain barrier by osmotic disruption; Consequent to hypertonic urea, the intracarotid infusion of norepinephrine, 50 ng/kg-min, significantly increase cerebral blood flow (49%), cerebral oxygen consumption (21%), and cerebral glucose uptake (76%), It appears probable that the cerebrovascular responses to norepinephrine are dependent on the integrity of the blood-brain barrier; It is likely that the increase in cerebral blood flow, associated with norepinephrine when it bypasses the barrier, is secondary to an increase in cerebral metabolism.", "PMID": 961901}
{"id": "pubmed23n0032_4745", "title": "Influence of endogenous norepinephrine on cerebral blood flow and metabolism.", "content": "The influence of brain norepinephrine on cerebral metabolism and blood flow was examined because exogenous norepinephrine, administered in a way that the blood-brain barrier is bypassed, has been shown to effect pronounced changes in the cerebral circulation. Reserpine (40 mug/kg, by intracarotid infusion) was administered in order to release brain norepinephrine in five anesthetized baboons. Reserpine significantly increased cerebral oxygen consumption (23%) and cerebral blood flow (50%). This response lasted for approximately 60 min. In a further  five animals, effects of central beta-adrenoreceptor blockade were studied. Pro pranolol (12 mug/kg-min) produced an immediate, significant reduction in both cerebral oxygen consumption (40%) and cerebral glucose uptake (39%). Cerebral blood flow was reduced minimally. However, the responsiveness of the cerebral circulation to induced hypercapnia was severely attenuated from a gradient of 3.22 before, to 1,11 after, administration. These experiments suggest that central norepinephrine can influence the cerebral circulation primarily through noradrenergic effects on brain metabolism.", "contents": "Influence of endogenous norepinephrine on cerebral blood flow and metabolism. The influence of brain norepinephrine on cerebral metabolism and blood flow was examined because exogenous norepinephrine, administered in a way that the blood-brain barrier is bypassed, has been shown to effect pronounced changes in the cerebral circulation. Reserpine (40 mug/kg, by intracarotid infusion) was administered in order to release brain norepinephrine in five anesthetized baboons. Reserpine significantly increased cerebral oxygen consumption (23%) and cerebral blood flow (50%). This response lasted for approximately 60 min. In a further  five animals, effects of central beta-adrenoreceptor blockade were studied. Pro pranolol (12 mug/kg-min) produced an immediate, significant reduction in both cerebral oxygen consumption (40%) and cerebral glucose uptake (39%). Cerebral blood flow was reduced minimally. However, the responsiveness of the cerebral circulation to induced hypercapnia was severely attenuated from a gradient of 3.22 before, to 1,11 after, administration. These experiments suggest that central norepinephrine can influence the cerebral circulation primarily through noradrenergic effects on brain metabolism.", "PMID": 961902}
{"id": "pubmed23n0032_4746", "title": "Frank-Starling relationship as an important determinant of fetal cardiac output.", "content": "The importance of the Frank-Starling mechanism was evaluated in seven chronically instrumented fetal lambs (128-141 days gestation). Continuous determinations of left ventricular (LV) internal dimensions and pressures were obtained while LV end-diastolic diameter (LVEDD) was reduced by superior vena cava occlusion and increased by infusion of fetal blood into left atrium. A highly significant relationship was found to exist between stroke volume and LV extent of shortening (delta D) (r = + 0.99, P less than 0.001). Altering LVEDD from 10,5 to 13mm or LV end-diastolic pressure from 2.5 to 8 mmHg resulted in a 68% augmentation, in delta D. Spontaneous respiratory efforts resulted in frequent beat-to-beat variations in LVEDD and delta D, which maintained cardiac output constant over a wide range of respiratory rates. Moreover, LV output determined by indicator-dilution techniques remained unchanged over a wide range of spontaneous heart rates (114-180 beats/min) as a result of changes in delta D appropriate to alterations in LVEDD. Thus, changes in resting myocardial fiber length are of fundamental importance in fetal cardiovascular homeostasis and, within physiologic limits, it is quite clear that the Frank-Starling mechanism is operative and effective in the fetal lamb;", "contents": "Frank-Starling relationship as an important determinant of fetal cardiac output. The importance of the Frank-Starling mechanism was evaluated in seven chronically instrumented fetal lambs (128-141 days gestation). Continuous determinations of left ventricular (LV) internal dimensions and pressures were obtained while LV end-diastolic diameter (LVEDD) was reduced by superior vena cava occlusion and increased by infusion of fetal blood into left atrium. A highly significant relationship was found to exist between stroke volume and LV extent of shortening (delta D) (r = + 0.99, P less than 0.001). Altering LVEDD from 10,5 to 13mm or LV end-diastolic pressure from 2.5 to 8 mmHg resulted in a 68% augmentation, in delta D. Spontaneous respiratory efforts resulted in frequent beat-to-beat variations in LVEDD and delta D, which maintained cardiac output constant over a wide range of respiratory rates. Moreover, LV output determined by indicator-dilution techniques remained unchanged over a wide range of spontaneous heart rates (114-180 beats/min) as a result of changes in delta D appropriate to alterations in LVEDD. Thus, changes in resting myocardial fiber length are of fundamental importance in fetal cardiovascular homeostasis and, within physiologic limits, it is quite clear that the Frank-Starling mechanism is operative and effective in the fetal lamb;", "PMID": 961903}
{"id": "pubmed23n0032_4747", "title": "Ion transport and structure of urinary bladder epithelium of Amphiuma.", "content": "The urinary bladder of Amphiuma exhibits stable transport properties and an electrical potential difference in vitro. The lumen is significantly negative to the serosa and under short-circuited conditions flux rations for Na and Cl of 5.92 +/- 0.42 and 1.81 +/- 0.20, respectively, were observed. The close agreement between the short-circuit current and net Na flux suggests that most, if not all, of the current is carried by Na. Both ouabain and amiloride decreased the short-circuit current and the mucosal-to-serosal (M leads to S) flux of Na. Furosemide caused a transient increase in the M leads to S flux of Na and Cl but ADH was without effect. In bladders that had high transmural resistance, a net movement of K in the M leads to S direction under short-circuited conditions with flux ratios of up to 2 could be observed. The epithelium of the Amphiuma bladder consists of three cell types: granular cells, basal cells, and mitochondria-rich cells. No goblet cells are present. The mitochondria-rich cells comprise less than 5% of the population of the surface epithelium in Amphiuma in contrast to other amphibian bladders, where it accounts for up to 25% of the population.", "contents": "Ion transport and structure of urinary bladder epithelium of Amphiuma. The urinary bladder of Amphiuma exhibits stable transport properties and an electrical potential difference in vitro. The lumen is significantly negative to the serosa and under short-circuited conditions flux rations for Na and Cl of 5.92 +/- 0.42 and 1.81 +/- 0.20, respectively, were observed. The close agreement between the short-circuit current and net Na flux suggests that most, if not all, of the current is carried by Na. Both ouabain and amiloride decreased the short-circuit current and the mucosal-to-serosal (M leads to S) flux of Na. Furosemide caused a transient increase in the M leads to S flux of Na and Cl but ADH was without effect. In bladders that had high transmural resistance, a net movement of K in the M leads to S direction under short-circuited conditions with flux ratios of up to 2 could be observed. The epithelium of the Amphiuma bladder consists of three cell types: granular cells, basal cells, and mitochondria-rich cells. No goblet cells are present. The mitochondria-rich cells comprise less than 5% of the population of the surface epithelium in Amphiuma in contrast to other amphibian bladders, where it accounts for up to 25% of the population.", "PMID": 961904}
{"id": "pubmed23n0032_4748", "title": "Urate reabsorption in proximal convoluted tubule of the rat kidney.", "content": "Urate reabsorption was examined in the rat utilizing clearance and in vivo microperfusion techniques. In control rats, the fractional excretion of urate averaged 12.5 +/- 1.3% of the filtered load, with 60-70% of perfused urate reabsorbed at 2 mm of perfused tubule length (urate efflux). Increasing the perfusion concentration of urate to 6, 12, and 25 mg/100 ml did not alter the fractional rate of reabsorption. The acute administration of chlorothiazide resulted in a fall in GFR and Curate such that the fractional excretion was unchanged from controls and averaged 13.3 +/- 2.0%, without an associated change in urate reabsorption from proximal perfusates despite the presence of significant inhibition of sodium and water reabsorption. By contrast, the chronic administration of chlorothiazide accompanied by a low-sodium diet resulted in a significantly lower fractions excretion rate of urate of 7.95 +/- 0.5% and a significant increase in reabsorption of sodium and water as well as urate from microperfusates, In control rats receiving an infusion of 5% mannitol in isotonic saline, urate secretion was demonstrated by the urinary precession of [2-14C]urate from [methoxy-3H]inulin following placement of these isotopes on the surface of the kidney. The additional infusion of chlorothiazide did not alter this pattern of isotope recovery in the urine.", "contents": "Urate reabsorption in proximal convoluted tubule of the rat kidney. Urate reabsorption was examined in the rat utilizing clearance and in vivo microperfusion techniques. In control rats, the fractional excretion of urate averaged 12.5 +/- 1.3% of the filtered load, with 60-70% of perfused urate reabsorbed at 2 mm of perfused tubule length (urate efflux). Increasing the perfusion concentration of urate to 6, 12, and 25 mg/100 ml did not alter the fractional rate of reabsorption. The acute administration of chlorothiazide resulted in a fall in GFR and Curate such that the fractional excretion was unchanged from controls and averaged 13.3 +/- 2.0%, without an associated change in urate reabsorption from proximal perfusates despite the presence of significant inhibition of sodium and water reabsorption. By contrast, the chronic administration of chlorothiazide accompanied by a low-sodium diet resulted in a significantly lower fractions excretion rate of urate of 7.95 +/- 0.5% and a significant increase in reabsorption of sodium and water as well as urate from microperfusates, In control rats receiving an infusion of 5% mannitol in isotonic saline, urate secretion was demonstrated by the urinary precession of [2-14C]urate from [methoxy-3H]inulin following placement of these isotopes on the surface of the kidney. The additional infusion of chlorothiazide did not alter this pattern of isotope recovery in the urine.", "PMID": 961905}
{"id": "pubmed23n0032_4749", "title": "Influence of sympathetic nerves on oxygen uptake or resting mammalian skeletal muscle,.", "content": "Gracilis muscles of anesthetized dogs were isolated and perfused with blood at constant flow. Oxygen uptake (Vo2) was measured by spectrophotometry of paired arterial and venous samples and monitored by continuous recording of HbO2 saturation. The sympathetic chain, connected to the isolated muscles, was stimulated electrically with supramaximal pulses. High frequencies (8-16/s) increased vascular resistance (R, ratio to control 1.86 +/- 0.6 SEM) and decreased Vo2 (0.61 +/- 0.02). Both effects could be maintained 10 min or longer. After stimulation there was an increase in Vo2, which could be less than equal to, or greater than the O2 deficit, depending on the stimulation frequency, the period of stimulation, and the blood flow. Low frequencies (0.5-4/s) increased R (1.39 +/- 0.04) and increased Vo2 during (1,18 +/- 0.03) and after stimulation. In several experiments there was an initial decrease in Vo2 which changed to an increase during the stimulation period while R remained elevated. These observations indicate that the decrease in Vo2 is due to closure of precapillary sphincters, whereas the increase is a direct effect on cell metabolism.", "contents": "Influence of sympathetic nerves on oxygen uptake or resting mammalian skeletal muscle,. Gracilis muscles of anesthetized dogs were isolated and perfused with blood at constant flow. Oxygen uptake (Vo2) was measured by spectrophotometry of paired arterial and venous samples and monitored by continuous recording of HbO2 saturation. The sympathetic chain, connected to the isolated muscles, was stimulated electrically with supramaximal pulses. High frequencies (8-16/s) increased vascular resistance (R, ratio to control 1.86 +/- 0.6 SEM) and decreased Vo2 (0.61 +/- 0.02). Both effects could be maintained 10 min or longer. After stimulation there was an increase in Vo2, which could be less than equal to, or greater than the O2 deficit, depending on the stimulation frequency, the period of stimulation, and the blood flow. Low frequencies (0.5-4/s) increased R (1.39 +/- 0.04) and increased Vo2 during (1,18 +/- 0.03) and after stimulation. In several experiments there was an initial decrease in Vo2 which changed to an increase during the stimulation period while R remained elevated. These observations indicate that the decrease in Vo2 is due to closure of precapillary sphincters, whereas the increase is a direct effect on cell metabolism.", "PMID": 961906}
{"id": "pubmed23n0032_4750", "title": "Noncompensation of adipose mass in partially lipectomized mice and rats.", "content": "The effects of surgical ablation of adipose tissue were studied in normal mice and rats. It was found that: 1) restortation of adipose tissue does not occur locally in epididymal fat pads of young rats. 2) Bilateral epididymal fat pad removal in mice disrupts the testes and causes the other fat depots to accumulate excess lipid, but these effects are not sustained; After a sufficient recovery period, testes appear normal and no excess lipid is found in the remaining depots; 3) Temporary enlargement of remaining depots is probably due specifically to epididymal pad removal. It does not occur in response to inguinal depot removal, nor in response to disruption of the testes alone; 4)The quantity of lipid stored by a rapidly growing mouse depends on the number of intact depots in the mouse. These results suggest that surgical removal of fat does not lead to compensatory growth of fat. Autoregulation of adipose tissue mass, if it occurs, most likely operates through detection of adipocyte size rather than adipocyte number or total fat mass.", "contents": "Noncompensation of adipose mass in partially lipectomized mice and rats. The effects of surgical ablation of adipose tissue were studied in normal mice and rats. It was found that: 1) restortation of adipose tissue does not occur locally in epididymal fat pads of young rats. 2) Bilateral epididymal fat pad removal in mice disrupts the testes and causes the other fat depots to accumulate excess lipid, but these effects are not sustained; After a sufficient recovery period, testes appear normal and no excess lipid is found in the remaining depots; 3) Temporary enlargement of remaining depots is probably due specifically to epididymal pad removal. It does not occur in response to inguinal depot removal, nor in response to disruption of the testes alone; 4)The quantity of lipid stored by a rapidly growing mouse depends on the number of intact depots in the mouse. These results suggest that surgical removal of fat does not lead to compensatory growth of fat. Autoregulation of adipose tissue mass, if it occurs, most likely operates through detection of adipocyte size rather than adipocyte number or total fat mass.", "PMID": 961907}
{"id": "pubmed23n0032_4751", "title": "Afterload-induced homeometric autoregulation in isolated cardiac muscle.", "content": "Pressure-induced homeometric autoregulation (HAR) has been demonstrated by many investigators in the mammalian ventricle; In isolated cardiac muscle, however, several investigators have reported the opposite effect (anti-HAR); namely, that the first beat after a transition from isotonic to isometric contraction is the most forceful, with a decline over several beats to a steady state. In the present study we find that trabeculae from the canine right ventricle demonstrate either HAR or anti-HAR, depending on the rate of stimulation, the calcium level, and the temperature. Higher calcium, higher temperature, and lower rate of stimulation produce either less HAR or more anti-HAR. When only temperature and rate of stimulation are varied, in each muscle there is a unique rate for each temperature above which HAR occurs and below which anti-HAR occurs.", "contents": "Afterload-induced homeometric autoregulation in isolated cardiac muscle. Pressure-induced homeometric autoregulation (HAR) has been demonstrated by many investigators in the mammalian ventricle; In isolated cardiac muscle, however, several investigators have reported the opposite effect (anti-HAR); namely, that the first beat after a transition from isotonic to isometric contraction is the most forceful, with a decline over several beats to a steady state. In the present study we find that trabeculae from the canine right ventricle demonstrate either HAR or anti-HAR, depending on the rate of stimulation, the calcium level, and the temperature. Higher calcium, higher temperature, and lower rate of stimulation produce either less HAR or more anti-HAR. When only temperature and rate of stimulation are varied, in each muscle there is a unique rate for each temperature above which HAR occurs and below which anti-HAR occurs.", "PMID": 961908}
{"id": "pubmed23n0032_4752", "title": "Polyamine synthesis in liver and kidney of flounder in response to methylmercury.", "content": "The effect of methylmercury administration on polyamine synthesis was studied in the liver and kidney of the winter flounder (Pseudopleuronectes americanus). A single injection of methylmercury resulted in five- and sevenfold elevations of ornithine decarboxylase activity in the liver and kidney within 15 and 45 h, respectively. There were elevations of both putrescine- and spermidine-stimulated S-adenosylmethionine decarboxylase activities (approximately 1.5-fold) in both tissues. Evaluation of the polyamine accumulation patterns in these tissues indicated that in the liver all three polyamines increased in concentration until 48 h and then decline. In the kidney, the concentration of putrescine increased steadily until it was 200% of control at 72 h and then declined. Spermidine concentration decreased throughout the time studied and was 17% of control at 1 wk. There was no significant change in the concentration of spermine throughout the period studied. The changes in the polyamine pools and in the activities of the polyamine biosynthetic enzymes after methylmercury administration are consistent with an involvement of the polyamines in the recovery phase to a toxic dose of methylmercury.", "contents": "Polyamine synthesis in liver and kidney of flounder in response to methylmercury. The effect of methylmercury administration on polyamine synthesis was studied in the liver and kidney of the winter flounder (Pseudopleuronectes americanus). A single injection of methylmercury resulted in five- and sevenfold elevations of ornithine decarboxylase activity in the liver and kidney within 15 and 45 h, respectively. There were elevations of both putrescine- and spermidine-stimulated S-adenosylmethionine decarboxylase activities (approximately 1.5-fold) in both tissues. Evaluation of the polyamine accumulation patterns in these tissues indicated that in the liver all three polyamines increased in concentration until 48 h and then decline. In the kidney, the concentration of putrescine increased steadily until it was 200% of control at 72 h and then declined. Spermidine concentration decreased throughout the time studied and was 17% of control at 1 wk. There was no significant change in the concentration of spermine throughout the period studied. The changes in the polyamine pools and in the activities of the polyamine biosynthetic enzymes after methylmercury administration are consistent with an involvement of the polyamines in the recovery phase to a toxic dose of methylmercury.", "PMID": 961909}
{"id": "pubmed23n0032_4753", "title": "Effects of peptide hormone structure on H+ secretion by Necturus gastric mucosa.", "content": "The actions of human synthetic gastrin I(G), the C-terminal tetrapeptide of gastrin (T), and the C-terminal octapeptide of cholecystokinin (OP) on acid secretion and transepithelial potential difference (PD) of the isolated Necturus gastric mucosa were determined. All three peptides induced H+ secretion, but the maximum H+ output was less with OP than with G or T. G and OP produced their maximum H+ output at lower molar concentrations than T. G- and OP-stimulated secretion was long sustained, but T-stimulated secretion rapidly returned to basal levels. T- and G-stimulated secretion was partially inhibited by the addition of OP. Evidence is presented that T rapidly disappears from solutions exposed to gastric mucosa, whereas G does not. Washing sensitized the mucosa to subsequent addition of T. The results suggest that the action of the common C-terminal tetrapeptide of G, T, and OP is modified by the preceding amino acid sequences, and that T, the smallest of the three peptides, is rapidly degraded by gastric tissue in vitro. The implications of the work for the study of gastrointestinal hormone structure-function relationships in isolated tissue preparations are discussed.", "contents": "Effects of peptide hormone structure on H+ secretion by Necturus gastric mucosa. The actions of human synthetic gastrin I(G), the C-terminal tetrapeptide of gastrin (T), and the C-terminal octapeptide of cholecystokinin (OP) on acid secretion and transepithelial potential difference (PD) of the isolated Necturus gastric mucosa were determined. All three peptides induced H+ secretion, but the maximum H+ output was less with OP than with G or T. G and OP produced their maximum H+ output at lower molar concentrations than T. G- and OP-stimulated secretion was long sustained, but T-stimulated secretion rapidly returned to basal levels. T- and G-stimulated secretion was partially inhibited by the addition of OP. Evidence is presented that T rapidly disappears from solutions exposed to gastric mucosa, whereas G does not. Washing sensitized the mucosa to subsequent addition of T. The results suggest that the action of the common C-terminal tetrapeptide of G, T, and OP is modified by the preceding amino acid sequences, and that T, the smallest of the three peptides, is rapidly degraded by gastric tissue in vitro. The implications of the work for the study of gastrointestinal hormone structure-function relationships in isolated tissue preparations are discussed.", "PMID": 961910}
{"id": "pubmed23n0032_4754", "title": "Effects of potassium depletion on control of breathing in awake rats.", "content": "Possible mechanisms for the variable ventilatory response to metabolic acid-base disturbances have been examined in normal and K-depleted rats. Ventilatory measurements are correlated with CSF acid-base data. The ratios VE/VO2 and 1/PaCO2 are utilized as indices of alveolar ventilation. The log of these indices correlates closely with CSF [H+] independent of [K+] except at very low CSF [H+] where the change in log 1/PaCO2 and log VE/VO2 per change in CSF [H+] is much diminished in low-K rats. This finding suggests the presence of an additional stimulus to breathing in the low-K rat opposing the inhibitory effect of low CSF [H+]. Otherwise the chemical control of ventilation appears to be normal. However, low-K rats always breath with a low-flight-Vt pattern and occasionally with abnormal rhythms. The similarity of the low K breathing pattern to that reported in awake animals with vagotomy and pneumotaxic center (PC) lesions suggests that the altered breathing pattern in depletion involves vagal and/or PC pathways. The similarity of the low-K breathing pattern to that observed with reserpine administration together with the known relationships of K and catecholamine metabolism lead to the speculation that K depletion alters breathing via an effect on central catecholamine metabolism. However, other mechanisms involving changes in membrane excitability and intracellular pH in K depletion might also be involved.", "contents": "Effects of potassium depletion on control of breathing in awake rats. Possible mechanisms for the variable ventilatory response to metabolic acid-base disturbances have been examined in normal and K-depleted rats. Ventilatory measurements are correlated with CSF acid-base data. The ratios VE/VO2 and 1/PaCO2 are utilized as indices of alveolar ventilation. The log of these indices correlates closely with CSF [H+] independent of [K+] except at very low CSF [H+] where the change in log 1/PaCO2 and log VE/VO2 per change in CSF [H+] is much diminished in low-K rats. This finding suggests the presence of an additional stimulus to breathing in the low-K rat opposing the inhibitory effect of low CSF [H+]. Otherwise the chemical control of ventilation appears to be normal. However, low-K rats always breath with a low-flight-Vt pattern and occasionally with abnormal rhythms. The similarity of the low K breathing pattern to that reported in awake animals with vagotomy and pneumotaxic center (PC) lesions suggests that the altered breathing pattern in depletion involves vagal and/or PC pathways. The similarity of the low-K breathing pattern to that observed with reserpine administration together with the known relationships of K and catecholamine metabolism lead to the speculation that K depletion alters breathing via an effect on central catecholamine metabolism. However, other mechanisms involving changes in membrane excitability and intracellular pH in K depletion might also be involved.", "PMID": 961911}
{"id": "pubmed23n0032_4755", "title": "Renal responses of Australian lungfish to vasotocin, angiotensin II, and NaCl infusion.", "content": "The Australian lungfish (Neoceratodus forsteri) responds to intravenous injections of 0.63 ng/kg or more of arginine vasotocin with increased dorsal aortic blood pressure, inulin clearance, urine flow, and tubular rejection of Na+. Single injections of 1 ng/kg or more of angiotensin II or norepinephrine also increase dorsal aortic pressure but do not cause consistent diuresis and natriuresis, Continuous infusions of angiotensin II or repeated injections of norepinephrine produce sustained hypertension and more modest diuresis and natriuresis than are seen after injections of arginine vasotocin that cause less hypertension. Infusions of isosmolar or hyposmolar NaCl solutions increase blood pressure, inulin clearance, urine flow, and tubular Na+ rejection in a manner resembling the response to argininge vasotocoin injections. These data are consistent with the hypothesis that arginine vasotocin is released in response to volume expansion in lungfishes and that it may act on the kidney as a diuretic and natriuretic hormone. They do not rule out a more direct action of expansion on renal functions.", "contents": "Renal responses of Australian lungfish to vasotocin, angiotensin II, and NaCl infusion. The Australian lungfish (Neoceratodus forsteri) responds to intravenous injections of 0.63 ng/kg or more of arginine vasotocin with increased dorsal aortic blood pressure, inulin clearance, urine flow, and tubular rejection of Na+. Single injections of 1 ng/kg or more of angiotensin II or norepinephrine also increase dorsal aortic pressure but do not cause consistent diuresis and natriuresis, Continuous infusions of angiotensin II or repeated injections of norepinephrine produce sustained hypertension and more modest diuresis and natriuresis than are seen after injections of arginine vasotocin that cause less hypertension. Infusions of isosmolar or hyposmolar NaCl solutions increase blood pressure, inulin clearance, urine flow, and tubular Na+ rejection in a manner resembling the response to argininge vasotocoin injections. These data are consistent with the hypothesis that arginine vasotocin is released in response to volume expansion in lungfishes and that it may act on the kidney as a diuretic and natriuretic hormone. They do not rule out a more direct action of expansion on renal functions.", "PMID": 961912}
{"id": "pubmed23n0032_4756", "title": "Renal sugar transport in the winter flounder. I. Renal clearance studies.", "content": "The renal handling of several sugars was examined using clearance techniques in the winter flounder Pseudopleuronectes americanus. The nonmetabolizable sugar alpha-methyl-D-glucoside was extensively reabsorbed, with consequent accumulation in renal tissue to nearly twice plasma concentration. Both glucose and phlorizin abolished reabsorption and reduced tissue-to-plasma ratios (T/P). D-Galactose was reabsorbed. However, the T/P for free galactose was only 0.6 (total sugar was 1.7). Glucose and phlorizin produced only a transient decrease in reabsorption and no change in T/P. 2-Deoxy-D-glucose showed neither net reabsorption nore secretion. Nevertheless, kidney T/P were inexcess of 6 for total sugar and 1.2 for free sugar, indicating entry through the peritubular face of the tubule. Neither glucose nor phlorizin altered 2-deoxy-D-glucose clearance, but both reduced T/P for total sugar (2.4) and free sugar (0.7). Thus, several systems govern the handling of these sugars at the luminal membrane of the renal tubule, just as has been previously demonstrated at the peritubular membrane in this species.", "contents": "Renal sugar transport in the winter flounder. I. Renal clearance studies. The renal handling of several sugars was examined using clearance techniques in the winter flounder Pseudopleuronectes americanus. The nonmetabolizable sugar alpha-methyl-D-glucoside was extensively reabsorbed, with consequent accumulation in renal tissue to nearly twice plasma concentration. Both glucose and phlorizin abolished reabsorption and reduced tissue-to-plasma ratios (T/P). D-Galactose was reabsorbed. However, the T/P for free galactose was only 0.6 (total sugar was 1.7). Glucose and phlorizin produced only a transient decrease in reabsorption and no change in T/P. 2-Deoxy-D-glucose showed neither net reabsorption nore secretion. Nevertheless, kidney T/P were inexcess of 6 for total sugar and 1.2 for free sugar, indicating entry through the peritubular face of the tubule. Neither glucose nor phlorizin altered 2-deoxy-D-glucose clearance, but both reduced T/P for total sugar (2.4) and free sugar (0.7). Thus, several systems govern the handling of these sugars at the luminal membrane of the renal tubule, just as has been previously demonstrated at the peritubular membrane in this species.", "PMID": 961913}
{"id": "pubmed23n0032_4757", "title": "Renal sugar transport in the winter flounder. II. Galactose transport system.", "content": "The structural specificity of the transport of 0.5 mM D-galactose and 2-deoxy-D-galactose by teased renal tubules of the winter flounder Pseudopleuronectes americanus was investigated. The sugar uptake reflects preponderantly transport at the antiluminal membrane of tubular cells. Both sugars compete for the transport sites, indicating the sharing of a common carrier. 1) The structural requirements for the hexose-carrier interaction were defined by an inhibiton analysis using 12 structurally analogous sugars (5 mM): a (pyranose) ring structure; a free hydroxyl on C1; free hydroxyls (equatorial configuration) on C3 and C4; an oxygen on C6. Close packing in the vicinity of C2 is indicated. 2) Consonant with the requirement of a free C1-OH, beta-methyl-D-galactoside is not transported 3) Increasing concentrations (0.05-0.5 mM) of phlorizin and phloretin inhibit sugar uptake by lowering cellular levels of phosphorylated hexoses, whereas the levels of free sugars are not depressed. The possibility of an interaction of the sugar at C1-0H with a phosphorylated group at the carrier as the first step in the translocation process of both hexoses across the basal membrane is raised.", "contents": "Renal sugar transport in the winter flounder. II. Galactose transport system. The structural specificity of the transport of 0.5 mM D-galactose and 2-deoxy-D-galactose by teased renal tubules of the winter flounder Pseudopleuronectes americanus was investigated. The sugar uptake reflects preponderantly transport at the antiluminal membrane of tubular cells. Both sugars compete for the transport sites, indicating the sharing of a common carrier. 1) The structural requirements for the hexose-carrier interaction were defined by an inhibiton analysis using 12 structurally analogous sugars (5 mM): a (pyranose) ring structure; a free hydroxyl on C1; free hydroxyls (equatorial configuration) on C3 and C4; an oxygen on C6. Close packing in the vicinity of C2 is indicated. 2) Consonant with the requirement of a free C1-OH, beta-methyl-D-galactoside is not transported 3) Increasing concentrations (0.05-0.5 mM) of phlorizin and phloretin inhibit sugar uptake by lowering cellular levels of phosphorylated hexoses, whereas the levels of free sugars are not depressed. The possibility of an interaction of the sugar at C1-0H with a phosphorylated group at the carrier as the first step in the translocation process of both hexoses across the basal membrane is raised.", "PMID": 961914}
{"id": "pubmed23n0032_4758", "title": "Alanine metabolism and gluconeogenesis in the rat.", "content": "The metabolism of alanine and several other gluconegoneic substrates was studied in anesthtized fed and fasted rats, i.e., rats with low and high rates of gluconeogenesis. Glutamine was released by the hindquarter (muscle) in both groups, whereas lactate, pyruvate, and alanine were taken up in fed rats and were released during starvation. Despite this, blood levels of alanine, lactate, and pyruvate were diminished in fasting rats, suggesting increased extraction by liver. Treatment of fasted rats for 24 h with phloridzin caused glycosuria and secondarily led to hypoglycemia and an intensification of the chargesobserved with fasting, i.e., hyperketonemia, hyperglucagonemia, and increased gluconeogenesis (assessed by urea N excretion). Blood alanine was decreased, even though the release of alanine from muscle was increased. Pretreatment with triamcinolone and administration of exogenous alanine both attenuated the hypoglycemia and ketosis, It is concluded that 1) in states of heightened gluconeogenesis, alanine release from muscle may not keep pace with extraction by liver and blood alanine decreases; 2) the release of alanine, lactate, and pyruvate from muscle parallel each other suggesting common control factors; and 3) in the red state muscle is an important site of lactate disposition.", "contents": "Alanine metabolism and gluconeogenesis in the rat. The metabolism of alanine and several other gluconegoneic substrates was studied in anesthtized fed and fasted rats, i.e., rats with low and high rates of gluconeogenesis. Glutamine was released by the hindquarter (muscle) in both groups, whereas lactate, pyruvate, and alanine were taken up in fed rats and were released during starvation. Despite this, blood levels of alanine, lactate, and pyruvate were diminished in fasting rats, suggesting increased extraction by liver. Treatment of fasted rats for 24 h with phloridzin caused glycosuria and secondarily led to hypoglycemia and an intensification of the chargesobserved with fasting, i.e., hyperketonemia, hyperglucagonemia, and increased gluconeogenesis (assessed by urea N excretion). Blood alanine was decreased, even though the release of alanine from muscle was increased. Pretreatment with triamcinolone and administration of exogenous alanine both attenuated the hypoglycemia and ketosis, It is concluded that 1) in states of heightened gluconeogenesis, alanine release from muscle may not keep pace with extraction by liver and blood alanine decreases; 2) the release of alanine, lactate, and pyruvate from muscle parallel each other suggesting common control factors; and 3) in the red state muscle is an important site of lactate disposition.", "PMID": 961915}
{"id": "pubmed23n0032_4759", "title": "Organic solutes in fluid absorption by renal proximal convoluted tubules.", "content": "Proximal convoluted tubules were dissected from rabbit kidneys and perfused with artificial solutions in vitro. The effect of various organic solutes on rate of fluid absorption and transepithelial voltage was tested by removing solutes from or adding them to perfusate and/or bath. Omission of albumin from the bath caused rate of fluid absorption to descrease 33% without any change in voltage. Omission of glucose, lactate, alanine, and citrate from the bath had no effect. In contrast, when they were removed from perfusate, rate of fluid absorption fell by 45-75% (depending on whether they were replaced by NaCl or mannitol and NaCl), and voltage (normally negative in lymen) decreased to near zero. Adding glucose or alanine individually to perfusate caused a small increase in rate of fluid absorption and a relatively large increase in voltage. alpha-Methyl-D-glucoside and cycloleucine (which are transported but not metabolized) had effects similar to glucose and alanine, except that voltage changes were not as great. Phlorizin (10(-5) M in perfusate) had the same effect as removing glucose from perfusate. When glucose and alanine were added to perfusate, epithelial cell swelled significantly. Lactate and citrate also caused rate of fluid absorption to increase when they were added to perfusate, but they did not affect transepithelial voltage nor did they cause cells to swell significantly. Possible mechanisms of these effects and the role of organic solutes in fluid absorption by proximal convoluted tubules are discussed.", "contents": "Organic solutes in fluid absorption by renal proximal convoluted tubules. Proximal convoluted tubules were dissected from rabbit kidneys and perfused with artificial solutions in vitro. The effect of various organic solutes on rate of fluid absorption and transepithelial voltage was tested by removing solutes from or adding them to perfusate and/or bath. Omission of albumin from the bath caused rate of fluid absorption to descrease 33% without any change in voltage. Omission of glucose, lactate, alanine, and citrate from the bath had no effect. In contrast, when they were removed from perfusate, rate of fluid absorption fell by 45-75% (depending on whether they were replaced by NaCl or mannitol and NaCl), and voltage (normally negative in lymen) decreased to near zero. Adding glucose or alanine individually to perfusate caused a small increase in rate of fluid absorption and a relatively large increase in voltage. alpha-Methyl-D-glucoside and cycloleucine (which are transported but not metabolized) had effects similar to glucose and alanine, except that voltage changes were not as great. Phlorizin (10(-5) M in perfusate) had the same effect as removing glucose from perfusate. When glucose and alanine were added to perfusate, epithelial cell swelled significantly. Lactate and citrate also caused rate of fluid absorption to increase when they were added to perfusate, but they did not affect transepithelial voltage nor did they cause cells to swell significantly. Possible mechanisms of these effects and the role of organic solutes in fluid absorption by proximal convoluted tubules are discussed.", "PMID": 961916}
{"id": "pubmed23n0032_4760", "title": "Osmotic interaction of plasma proteins with interstitial macromolecules.", "content": "The osmotic interaction of plasma proteins with collagen and hyaluronate has been evaluated by measuring the oncotic pressure of mixed solutions of varying composition. Collagen, despite its insolubility, exhibits a pronounced volume exclusion effect on plasma proteins, and the oncotic pressure of mixed solutions is considerably higher than that of the plasma protein stock solution. The volume exclusion of collagen on small molecules such as sucrose is negligible. A solution composed of 1.6% plasma proteins, 20% collagen, and .4% hyaluronate in Ringer solution, approximating the composition of the interstitium, was found to yield higher oncotic pressures than those previously reported from the interstitium. The probable role of impurities and degradation in the isolation process is discussed. Results reported earlier from in vitro and in vivo studies indicated that tissue oncotic pressures are considerably higher than generally recognized and that tissue fluid is in probable osmotic equilibrium with lymph in skin and muscle.", "contents": "Osmotic interaction of plasma proteins with interstitial macromolecules. The osmotic interaction of plasma proteins with collagen and hyaluronate has been evaluated by measuring the oncotic pressure of mixed solutions of varying composition. Collagen, despite its insolubility, exhibits a pronounced volume exclusion effect on plasma proteins, and the oncotic pressure of mixed solutions is considerably higher than that of the plasma protein stock solution. The volume exclusion of collagen on small molecules such as sucrose is negligible. A solution composed of 1.6% plasma proteins, 20% collagen, and .4% hyaluronate in Ringer solution, approximating the composition of the interstitium, was found to yield higher oncotic pressures than those previously reported from the interstitium. The probable role of impurities and degradation in the isolation process is discussed. Results reported earlier from in vitro and in vivo studies indicated that tissue oncotic pressures are considerably higher than generally recognized and that tissue fluid is in probable osmotic equilibrium with lymph in skin and muscle.", "PMID": 961917}
{"id": "pubmed23n0032_4761", "title": "Renal tubular reabsorptive response to hypernatremia.", "content": "Renal tubular reabsorptive response to rapid infusions of isotonic saline and 5% NaCl solutions were measured during brisk ethacrynic acid diuresis in anesthetized dogs. When adjustments were made for effects of variations in volume expansion, as indexed by plasma protein concentration ([Pprot]), tubular reabsorption of sodium per unit filtrate volume (TNa/GFR) was found to be significantly and positively correlated with plasma sodium concentration ([PNa]) despite hypernatremia and total body surfeit of sodium. The proportions of sodium and water reabsorbed were also homeostatically inappropriate, since the sodium concentration in the reabsorbate was somewhat in excess of that in contemporary plasma ultrafiltrate. These findings signify that glomerulotubular balance holds when the filtered load of sodium is increased by an increment in [pNa] as well as GFR. It is proposed that the moiety of tubular reabsorption (some 75% of GFR) studied here is more closely related to regulation of volume than of osmolality of sodium concentration, and the primary regulation exerted is on tubular volume reabsorption (bulk fluid reabsorption) rather than on the amount of sodium reabsorbed.", "contents": "Renal tubular reabsorptive response to hypernatremia. Renal tubular reabsorptive response to rapid infusions of isotonic saline and 5% NaCl solutions were measured during brisk ethacrynic acid diuresis in anesthetized dogs. When adjustments were made for effects of variations in volume expansion, as indexed by plasma protein concentration ([Pprot]), tubular reabsorption of sodium per unit filtrate volume (TNa/GFR) was found to be significantly and positively correlated with plasma sodium concentration ([PNa]) despite hypernatremia and total body surfeit of sodium. The proportions of sodium and water reabsorbed were also homeostatically inappropriate, since the sodium concentration in the reabsorbate was somewhat in excess of that in contemporary plasma ultrafiltrate. These findings signify that glomerulotubular balance holds when the filtered load of sodium is increased by an increment in [pNa] as well as GFR. It is proposed that the moiety of tubular reabsorption (some 75% of GFR) studied here is more closely related to regulation of volume than of osmolality of sodium concentration, and the primary regulation exerted is on tubular volume reabsorption (bulk fluid reabsorption) rather than on the amount of sodium reabsorbed.", "PMID": 961918}
{"id": "pubmed23n0032_4762", "title": "Subcellular membrane fatty acids of rat heart after cold acclimation or thyroxine.", "content": "Relative percent changes in fatty acid (FA) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. Rats in both environmental groups received thyroxine (T4) (1 mg/kg per day) or propylthiouracil (PTU) (5 mg/rat per day) for 10 days prior to sacrifice. Mitochondrial total unsaturated fatty acids (TUFA) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals receiving PTU. In mitochondria of the T4-treated warm-acclimated group, the relative content of linoleate decreased and that of stearate increased. Heart microsomes from warm-acclimated rats receiving T4 showed large increases in the percentage content of palmitate and stearate accompanied by reductions in linoleate and arachidonate. In cold-acclimated control rats, the relative content of stearate was elevated in both membrane fractions. The results suggested that thyroid hormones may decrease the TUFA composition of heart subcellular membranes by a relative increase in percent stearic acid and decrease in percent linoleic acid. Similar FA changes in these membranes in cold-acclimated rats may be related to the action of thyroid hormones.", "contents": "Subcellular membrane fatty acids of rat heart after cold acclimation or thyroxine. Relative percent changes in fatty acid (FA) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. Rats in both environmental groups received thyroxine (T4) (1 mg/kg per day) or propylthiouracil (PTU) (5 mg/rat per day) for 10 days prior to sacrifice. Mitochondrial total unsaturated fatty acids (TUFA) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals receiving PTU. In mitochondria of the T4-treated warm-acclimated group, the relative content of linoleate decreased and that of stearate increased. Heart microsomes from warm-acclimated rats receiving T4 showed large increases in the percentage content of palmitate and stearate accompanied by reductions in linoleate and arachidonate. In cold-acclimated control rats, the relative content of stearate was elevated in both membrane fractions. The results suggested that thyroid hormones may decrease the TUFA composition of heart subcellular membranes by a relative increase in percent stearic acid and decrease in percent linoleic acid. Similar FA changes in these membranes in cold-acclimated rats may be related to the action of thyroid hormones.", "PMID": 961919}
{"id": "pubmed23n0032_4763", "title": "The identification and treatment of adult brain dysfunction.", "content": "The authors hypothesize that adults who have had minimal brain dysfunction as children constitute a distinct diagnostic entity, adult brain dysfunction (ABD), which may exist alone or with a variety of other psychiatric syndromes. Patients with ABD share a basic impairment in ability to focus attention effectively; they may have different personality structures, symptom complexes, and behavioral patterns. However, with medication that corrects this impairment, remarkable improvement in the presenting symptoms and in the ability to work in therapy may occur, leading to changes in personality structure and behavior.", "contents": "The identification and treatment of adult brain dysfunction. The authors hypothesize that adults who have had minimal brain dysfunction as children constitute a distinct diagnostic entity, adult brain dysfunction (ABD), which may exist alone or with a variety of other psychiatric syndromes. Patients with ABD share a basic impairment in ability to focus attention effectively; they may have different personality structures, symptom complexes, and behavioral patterns. However, with medication that corrects this impairment, remarkable improvement in the presenting symptoms and in the ability to work in therapy may occur, leading to changes in personality structure and behavior.", "PMID": 961920}
{"id": "pubmed23n0032_4764", "title": "Reversal of tricyclic-overdosage-induced central anticholinergic syndrome by physostigmine.", "content": "Although there is evidence that the delirium, stupor, coma, and seizure-like activity seen in overdosage with tricyclic antidepressants and antiparkinson drugs are due to the central anticholinergic activity of these agents, patients with overdosage of these drugs are still frequently misdiagnosed. The authors present a case of reversal of anticholinergic-drug-induced prolonged coma, myoclonus, and choreoathetosis by physostigmine. This report supports the anticholinergic basis of the clinical manifestations of such overdosages, provides information on the role of acetylcholine and dopamine in psychiatric and movement disorders, and illustrates dramatically the need for accurate diagnosis and treatment.", "contents": "Reversal of tricyclic-overdosage-induced central anticholinergic syndrome by physostigmine. Although there is evidence that the delirium, stupor, coma, and seizure-like activity seen in overdosage with tricyclic antidepressants and antiparkinson drugs are due to the central anticholinergic activity of these agents, patients with overdosage of these drugs are still frequently misdiagnosed. The authors present a case of reversal of anticholinergic-drug-induced prolonged coma, myoclonus, and choreoathetosis by physostigmine. This report supports the anticholinergic basis of the clinical manifestations of such overdosages, provides information on the role of acetylcholine and dopamine in psychiatric and movement disorders, and illustrates dramatically the need for accurate diagnosis and treatment.", "PMID": 961921}
{"id": "pubmed23n0032_4765", "title": "Marijuana and hostility in a small-group setting.", "content": "The authors used several indices to assess the relationship between marijuana and hostility as both inner affect and verbal behavior in a small-group setting. Marijuana subjects reported a small but statistically significant decrease in hostile feelings after the introduction of a frustration stimulus. They also showed significantly less verbal hostility than placebo subjects both before and after introduction of a frustration stimulus. The authors note that research findings on marijuana and hostility are not consistent and suggest a multidetermined relationship based on dose, environment, nature of the frustration stimulus, and intraindividual factors.", "contents": "Marijuana and hostility in a small-group setting. The authors used several indices to assess the relationship between marijuana and hostility as both inner affect and verbal behavior in a small-group setting. Marijuana subjects reported a small but statistically significant decrease in hostile feelings after the introduction of a frustration stimulus. They also showed significantly less verbal hostility than placebo subjects both before and after introduction of a frustration stimulus. The authors note that research findings on marijuana and hostility are not consistent and suggest a multidetermined relationship based on dose, environment, nature of the frustration stimulus, and intraindividual factors.", "PMID": 961922}
{"id": "pubmed23n0032_4766", "title": "The treatment of anorexia nervosa.", "content": "The authors report on their treatment of 32 young patients with severe anorexia nervosa who had lost an average of 37% of their original body weight. The therapeutic approach, conceived to meet both the physiological and the psychological needs of the patients, was carried out in a psychiatric unit for adolescents in a general hospital and involved separation of the patient from his or her family, treatment of the malnutrition, individually planned psychotherapy, and concurrent work with the family. The importance of accepting the patient's uniqueness, maintaining long-term contact, and encouraging the patient's striving toward constructive growth and autonomy is emphasized.", "contents": "The treatment of anorexia nervosa. The authors report on their treatment of 32 young patients with severe anorexia nervosa who had lost an average of 37% of their original body weight. The therapeutic approach, conceived to meet both the physiological and the psychological needs of the patients, was carried out in a psychiatric unit for adolescents in a general hospital and involved separation of the patient from his or her family, treatment of the malnutrition, individually planned psychotherapy, and concurrent work with the family. The importance of accepting the patient's uniqueness, maintaining long-term contact, and encouraging the patient's striving toward constructive growth and autonomy is emphasized.", "PMID": 961923}
{"id": "pubmed23n0032_4767", "title": "Neuropsychological and EEG disturbances in polydrug users.", "content": "EEG and neuropsychological evaluation of 66 polydrug users revealed that 43% had EEG abnormalities and 45% had neuropsychological impairment 3 weeks after admission to a polydrug study unit. At 5-month follow-up, 27% of 30 retested subjects were still impaired neuropsychologically. Impairment may be related to extensive involvement with sedatives, alcohol, or heavy polydrug use. The authors suggest that cerebral dysfunction in polydrug users might be the result of organicity of intermediate duration and that deficits may be experienced by some beyond 5 months of reduced use or abstinence. Organicity may dictate structured, reality-based intervention techniques, especially early in treatment.", "contents": "Neuropsychological and EEG disturbances in polydrug users. EEG and neuropsychological evaluation of 66 polydrug users revealed that 43% had EEG abnormalities and 45% had neuropsychological impairment 3 weeks after admission to a polydrug study unit. At 5-month follow-up, 27% of 30 retested subjects were still impaired neuropsychologically. Impairment may be related to extensive involvement with sedatives, alcohol, or heavy polydrug use. The authors suggest that cerebral dysfunction in polydrug users might be the result of organicity of intermediate duration and that deficits may be experienced by some beyond 5 months of reduced use or abstinence. Organicity may dictate structured, reality-based intervention techniques, especially early in treatment.", "PMID": 961924}
{"id": "pubmed23n0032_4768", "title": "A description and evaluation of an outpatient intensive psychotherapy center.", "content": "The intensive psychotherapy center described and evaluated in this paper is unique because of its intensity and the fact that available treatment modalities are integrated into the program. Pateints are literally immersed in therapy on a full-time basis for an average of 3 weeks. Assessment of the program through the use of questionnaires completed by 335 patients at the time of discharge showed significant improvement in their presenting symptoms, interpersonal relationships, ability to work, and general level of comfort. An 8-month follow-up study of 171 patients revealed that this improvement was retained.", "contents": "A description and evaluation of an outpatient intensive psychotherapy center. The intensive psychotherapy center described and evaluated in this paper is unique because of its intensity and the fact that available treatment modalities are integrated into the program. Pateints are literally immersed in therapy on a full-time basis for an average of 3 weeks. Assessment of the program through the use of questionnaires completed by 335 patients at the time of discharge showed significant improvement in their presenting symptoms, interpersonal relationships, ability to work, and general level of comfort. An 8-month follow-up study of 171 patients revealed that this improvement was retained.", "PMID": 961925}
{"id": "pubmed23n0032_4769", "title": "Quality-of-care standards and professional norms.", "content": "Incursions upon professional prerogatives are increasing with the advent of new requirements for accoutability; this has stemmed in part from the government's enlarged role in paying for mental health services. The author discusses the effect of professional norms on the assessment of quality of care, including the concepts of risk and autonomy, observability and privacy, the perspective of time, and the appropriateness of the penalty. He offers some suggestions for the design and implementation of quality-of-care standards that he believes will benefit both professionals and administrators, as well as protect the patient.", "contents": "Quality-of-care standards and professional norms. Incursions upon professional prerogatives are increasing with the advent of new requirements for accoutability; this has stemmed in part from the government's enlarged role in paying for mental health services. The author discusses the effect of professional norms on the assessment of quality of care, including the concepts of risk and autonomy, observability and privacy, the perspective of time, and the appropriateness of the penalty. He offers some suggestions for the design and implementation of quality-of-care standards that he believes will benefit both professionals and administrators, as well as protect the patient.", "PMID": 961926}
{"id": "pubmed23n0032_4770", "title": "Mental health services in the public and private sectors.", "content": "Analysis of data on mental health service providers indicates that in 1971 the private sector accounted for 34% of inpatient days, 86% of outpatient visits, 44% of expenditures by source of funds, and 51% of expenditures by receipt of funds. The author believes that mental health professionals must familiarize themselves with the economic interests influencing national health insurance proposals and with public policy making processes if they are to help preserve appropriate roles for the public and private sectors in mental health service delivery.", "contents": "Mental health services in the public and private sectors. Analysis of data on mental health service providers indicates that in 1971 the private sector accounted for 34% of inpatient days, 86% of outpatient visits, 44% of expenditures by source of funds, and 51% of expenditures by receipt of funds. The author believes that mental health professionals must familiarize themselves with the economic interests influencing national health insurance proposals and with public policy making processes if they are to help preserve appropriate roles for the public and private sectors in mental health service delivery.", "PMID": 961927}
{"id": "pubmed23n0032_4771", "title": "Utilization of prepaid services by patients with psychiatric diagnoses.", "content": "The authors document and discuss the utilization of psychiatric services in a rural group practice before and after the institution of a prepaid health plan. They found that the utilization of psychiatric services increased dramatically during the first year of the plan's operation. The increase in outpatient utilization continued throughout the 3 years studied, but inpatient utilization decreased after the first year and later reached a level lower than that seen before the plan was instituted. One possible reason for the decline in inpatient utilization is that increased outpatient care prevents hospitalization.", "contents": "Utilization of prepaid services by patients with psychiatric diagnoses. The authors document and discuss the utilization of psychiatric services in a rural group practice before and after the institution of a prepaid health plan. They found that the utilization of psychiatric services increased dramatically during the first year of the plan's operation. The increase in outpatient utilization continued throughout the 3 years studied, but inpatient utilization decreased after the first year and later reached a level lower than that seen before the plan was instituted. One possible reason for the decline in inpatient utilization is that increased outpatient care prevents hospitalization.", "PMID": 961928}
{"id": "pubmed23n0032_4772", "title": "Milieu therapy: toward a definition for reimbursement.", "content": "The authors identify certain essential elements and processes within the milieu therapy approach that could provide the basis for reimbursement of this treatment modality under insurance. They also examine a philosophical framework, various guidelines, and a mechanism for accountability in the use of this form of treatment.", "contents": "Milieu therapy: toward a definition for reimbursement. The authors identify certain essential elements and processes within the milieu therapy approach that could provide the basis for reimbursement of this treatment modality under insurance. They also examine a philosophical framework, various guidelines, and a mechanism for accountability in the use of this form of treatment.", "PMID": 961929}
{"id": "pubmed23n0032_4773", "title": "Psychotherapy fees and residency training.", "content": "Many residents in psychiatry tend to ignore their patients' attitudes and behavior concerning payment of fees for psychotherapy. The authors report on a new fee policy program designed to earn additional revenue for a low-fee outpatient psychotherapy clinic; they stress the therapeutic importance of resident and patient attention to fee-related issues.", "contents": "Psychotherapy fees and residency training. Many residents in psychiatry tend to ignore their patients' attitudes and behavior concerning payment of fees for psychotherapy. The authors report on a new fee policy program designed to earn additional revenue for a low-fee outpatient psychotherapy clinic; they stress the therapeutic importance of resident and patient attention to fee-related issues.", "PMID": 961930}
{"id": "pubmed23n0032_4774", "title": "Albinism and schizophreniform psychosis: a pedigree study.", "content": "The author presents a pedigree study of albinism associated with schizophreniform psychosis and concludes that the evidence of an association between the two traits is strong; the evidence for genetic linkage is suggestive. The pineal hormone melatonin and the melanocyte-stimulating hormone inhibitory factor may be etiologic determinants of psychosis associated with albinism.", "contents": "Albinism and schizophreniform psychosis: a pedigree study. The author presents a pedigree study of albinism associated with schizophreniform psychosis and concludes that the evidence of an association between the two traits is strong; the evidence for genetic linkage is suggestive. The pineal hormone melatonin and the melanocyte-stimulating hormone inhibitory factor may be etiologic determinants of psychosis associated with albinism.", "PMID": 961931}
{"id": "pubmed23n0032_4775", "title": "Anticholinergic exacerbation of phenothiazine-induced extrapyramidal syndrome.", "content": "The authors used tremography to record the bilateral digital tremor of a patient showing extrapyramidal system effects in response to withdrawal from phenothiazines. They found that changes in the amplitude of tremor correlated with clinical ratings of extrapyramidal disturbance. Changes in the amplitude and in the spectrum of tremor were similar to the bipolar paradoxical changes seen during intoxication with an anticholinergic psychotogen.", "contents": "Anticholinergic exacerbation of phenothiazine-induced extrapyramidal syndrome. The authors used tremography to record the bilateral digital tremor of a patient showing extrapyramidal system effects in response to withdrawal from phenothiazines. They found that changes in the amplitude of tremor correlated with clinical ratings of extrapyramidal disturbance. Changes in the amplitude and in the spectrum of tremor were similar to the bipolar paradoxical changes seen during intoxication with an anticholinergic psychotogen.", "PMID": 961932}
{"id": "pubmed23n0032_4776", "title": "Rapid treatment of acute psychosis.", "content": "Twenty-four patients with acute functional psychoses were treated with intramuscular haloperidol in a three-hour period. There was almost complete remission of cardinal symptoms (thought disorder, hallucinations, and delusional activity) in this period for 11 patients. Acute dystonia, easily reversed, was the only significant side effect. The authors therefore suggest that outpatient management may be feasible and preferable in the treatment of some acute psychotic episodes.", "contents": "Rapid treatment of acute psychosis. Twenty-four patients with acute functional psychoses were treated with intramuscular haloperidol in a three-hour period. There was almost complete remission of cardinal symptoms (thought disorder, hallucinations, and delusional activity) in this period for 11 patients. Acute dystonia, easily reversed, was the only significant side effect. The authors therefore suggest that outpatient management may be feasible and preferable in the treatment of some acute psychotic episodes.", "PMID": 961933}
{"id": "pubmed23n0032_4777", "title": "Attitude of psychiatric residents toward patient requests.", "content": "The authors assessed residents' comfort in dealing with 14 previously identified types of patient requests in inpatient, outpatient, and acute service settings. First-year residents expressed significantly more discomfort than more experienced residents on 11 categories. They were also significantly less comfortable in the acute service than the inpatient setting. All residents expressed more comfort dealing with dynamic, psychological, and \"nonsevere\" requests. These findings suggest a need to teach residents specific therapeutic responses to various categories of requests and to evaluate the desirability of starting the residency experience on an acute service.", "contents": "Attitude of psychiatric residents toward patient requests. The authors assessed residents' comfort in dealing with 14 previously identified types of patient requests in inpatient, outpatient, and acute service settings. First-year residents expressed significantly more discomfort than more experienced residents on 11 categories. They were also significantly less comfortable in the acute service than the inpatient setting. All residents expressed more comfort dealing with dynamic, psychological, and \"nonsevere\" requests. These findings suggest a need to teach residents specific therapeutic responses to various categories of requests and to evaluate the desirability of starting the residency experience on an acute service.", "PMID": 961934}
{"id": "pubmed23n0032_4778", "title": "Treatement of excessive weight gain in patients taking lithium.", "content": "Six female primary affective disorder patients who had gained an average of 9.5 kg while taking lithium lost an average of 2.9 kg on a 10-day 900 calorie a day hospital diet containing 100 mEq of sodium per day. No evidence of lithium toxicity was observed on this regimen. There was no evidence that fluid retention played a major role in the weight gain.", "contents": "Treatement of excessive weight gain in patients taking lithium. Six female primary affective disorder patients who had gained an average of 9.5 kg while taking lithium lost an average of 2.9 kg on a 10-day 900 calorie a day hospital diet containing 100 mEq of sodium per day. No evidence of lithium toxicity was observed on this regimen. There was no evidence that fluid retention played a major role in the weight gain.", "PMID": 961935}
{"id": "pubmed23n0032_4779", "title": "Difficult issues in health planning, development, and review.", "content": "To give an overview of the kinds of issues expected to be encountered by the new Health Systems Agencies being established in conformity with Public Law 93-641, the authors draw from the experiences of the Comprehensive Health Planning agency and Regional Medical Program of an Upstate New York area. Problems faced together in the years 1972 to 1975 in health planning, development, review, and public policy are described. The unusual geographic and working relationship of these agencies makes them, together, a useful prototype of the HSA to examine.", "contents": "Difficult issues in health planning, development, and review. To give an overview of the kinds of issues expected to be encountered by the new Health Systems Agencies being established in conformity with Public Law 93-641, the authors draw from the experiences of the Comprehensive Health Planning agency and Regional Medical Program of an Upstate New York area. Problems faced together in the years 1972 to 1975 in health planning, development, review, and public policy are described. The unusual geographic and working relationship of these agencies makes them, together, a useful prototype of the HSA to examine.", "PMID": 961941}
{"id": "pubmed23n0032_4780", "title": "A Bayesian approach to health project estimation.", "content": "This paper illustrates how a Bayesian statistical approach was used to estimate the outcomes of the National Tuberculosis Program in India. Such an estimate, it is argued, is necessary for a proper judgement about a project's social usefulness. The process of medical care delivery is reduced to a set of conditioned probabilities. The numbers are estimated using as source material medical records, the results of medical research, and the opinion of experts. Bayesian methods of estimation are used and their value is discussed. The final discussion contains a brief treatment of the role of project analysis in public decision making. The place of Bayesian methods in project analysis is briefly illustrated, demonstrating their operational value in the field of public health decision making.", "contents": "A Bayesian approach to health project estimation. This paper illustrates how a Bayesian statistical approach was used to estimate the outcomes of the National Tuberculosis Program in India. Such an estimate, it is argued, is necessary for a proper judgement about a project's social usefulness. The process of medical care delivery is reduced to a set of conditioned probabilities. The numbers are estimated using as source material medical records, the results of medical research, and the opinion of experts. Bayesian methods of estimation are used and their value is discussed. The final discussion contains a brief treatment of the role of project analysis in public decision making. The place of Bayesian methods in project analysis is briefly illustrated, demonstrating their operational value in the field of public health decision making.", "PMID": 961942}
{"id": "pubmed23n0032_4781", "title": "Nursing assessment in industry.", "content": "In order to be able to offer nursing service to industry, a community health agency must have some knowledge of the industry and the daily problesm faced by both management and worker. The nursing process can serve as a framework for the gathering of necessary information and planning of sound care. The five-step nursing process, which includes assessment, diagnosis, planning, implementation and evaluation, is discussed and annotated model Assessment Guide for Nursing in Industry is given (Appendix A). The six areas from which information should be gathered when assessing an industry are the following: I. The community in which the industry is located: II. The industry, its historical development, policies, and projections; III. The plant or physical structure; IV. The working population; V. The industrial process of the plant; VII. The existing health program. Once the assessment is completed and a diagnosis formulated, services can be offered based on specific, defined needs.", "contents": "Nursing assessment in industry. In order to be able to offer nursing service to industry, a community health agency must have some knowledge of the industry and the daily problesm faced by both management and worker. The nursing process can serve as a framework for the gathering of necessary information and planning of sound care. The five-step nursing process, which includes assessment, diagnosis, planning, implementation and evaluation, is discussed and annotated model Assessment Guide for Nursing in Industry is given (Appendix A). The six areas from which information should be gathered when assessing an industry are the following: I. The community in which the industry is located: II. The industry, its historical development, policies, and projections; III. The plant or physical structure; IV. The working population; V. The industrial process of the plant; VII. The existing health program. Once the assessment is completed and a diagnosis formulated, services can be offered based on specific, defined needs.", "PMID": 961943}
{"id": "pubmed23n0032_4782", "title": "Efficacy information in contraceptive counseling: those little white lies.", "content": "Various procedures for estimating the effectiveness of a method of contraception have been devised. These measures, in turn, have been used in populations which differ widely in their propensity to use contraceptives properly. Therefore, a wide array of failure rates is available in the family planning literature. Unfortunately, because of differences in measurement and in choice of population, a random selection of these reported failure rates will not produce a consistent ordinal or cardinal ranking of methods by their effectiveness. Moreover, such a wide variety or reported rates permits the family planning practitioner to choose selectively in order to maximize the attractiveness of his favorite method(s). By surveying family planning personnel in two major cities, we found that they do indeen appear to place the methods they actively dispense in an extremely favorable position. Specifically, they are biased against the traditional contraceptives, foam and the condom. In this paper, we discuss the theoretical underpinnings of the measurement of effectiveness, report the apparent bias in the levels of contraceptive effectiveness reported to the patient, and finally, recommend a procedure for eliminating the jumble of rates in the literature and the consequent confusion among family planning personnel.", "contents": "Efficacy information in contraceptive counseling: those little white lies. Various procedures for estimating the effectiveness of a method of contraception have been devised. These measures, in turn, have been used in populations which differ widely in their propensity to use contraceptives properly. Therefore, a wide array of failure rates is available in the family planning literature. Unfortunately, because of differences in measurement and in choice of population, a random selection of these reported failure rates will not produce a consistent ordinal or cardinal ranking of methods by their effectiveness. Moreover, such a wide variety or reported rates permits the family planning practitioner to choose selectively in order to maximize the attractiveness of his favorite method(s). By surveying family planning personnel in two major cities, we found that they do indeen appear to place the methods they actively dispense in an extremely favorable position. Specifically, they are biased against the traditional contraceptives, foam and the condom. In this paper, we discuss the theoretical underpinnings of the measurement of effectiveness, report the apparent bias in the levels of contraceptive effectiveness reported to the patient, and finally, recommend a procedure for eliminating the jumble of rates in the literature and the consequent confusion among family planning personnel.", "PMID": 961944}
{"id": "pubmed23n0032_4783", "title": "Applications of a job classification system in occupational epidemiology.", "content": "An occupational preventive medicine program attempts to control exposure so workers experience no detrimental effect on health. In a chemically complex industry, the definition of exposure is difficult because of the many different chemicals used and produced, the many different jobs and processes with qualitatively different exposures, and the movement of workers from job to job. Jobs have therefore been grouped on the basis of process or product into functionally homogeneous categories called occupational titles (OT's). Work experience can now be quantified independent of exposure (or by the dominant toxicants in each OT) and compared to health outcomes. Examples are discussed of the application of OT's to studies of the mortality and morbidity experience in the rubber industry, and the development of dose-response relations.", "contents": "Applications of a job classification system in occupational epidemiology. An occupational preventive medicine program attempts to control exposure so workers experience no detrimental effect on health. In a chemically complex industry, the definition of exposure is difficult because of the many different chemicals used and produced, the many different jobs and processes with qualitatively different exposures, and the movement of workers from job to job. Jobs have therefore been grouped on the basis of process or product into functionally homogeneous categories called occupational titles (OT's). Work experience can now be quantified independent of exposure (or by the dominant toxicants in each OT) and compared to health outcomes. Examples are discussed of the application of OT's to studies of the mortality and morbidity experience in the rubber industry, and the development of dose-response relations.", "PMID": 961945}
{"id": "pubmed23n0032_4784", "title": "Radiation therapy among A-bomb survivors.", "content": "Survey of hospitals and clinics where atomic bomb survivors and their comparisons reported having received radiation therapy confirmed that 137 were so treated. The malignancies of five subjects were possibly related to their earlier radiation therapy rather than their atomic bomb radiation exposure, stressing the importance of recording all medical X-ray exposures as a potential variable in epidemiological studies of cancer etiology.", "contents": "Radiation therapy among A-bomb survivors. Survey of hospitals and clinics where atomic bomb survivors and their comparisons reported having received radiation therapy confirmed that 137 were so treated. The malignancies of five subjects were possibly related to their earlier radiation therapy rather than their atomic bomb radiation exposure, stressing the importance of recording all medical X-ray exposures as a potential variable in epidemiological studies of cancer etiology.", "PMID": 961946}
{"id": "pubmed23n0032_4785", "title": "The use of utilization review records as a source of data on nursing home care.", "content": "Data form the Utah nursing home Utilization Review Program, 1970-73, provide a description of the population of nursing home patients in Utah and changes over time in patient medication and measures of activity. These data are analyzed according to type of care in home: personal, intermediate, or comprehensive. The collection of data in utilization review programs can help to evaluate the quality of care as well as appropriateness of placement, provided the information collected is designed with these ends in mind.", "contents": "The use of utilization review records as a source of data on nursing home care. Data form the Utah nursing home Utilization Review Program, 1970-73, provide a description of the population of nursing home patients in Utah and changes over time in patient medication and measures of activity. These data are analyzed according to type of care in home: personal, intermediate, or comprehensive. The collection of data in utilization review programs can help to evaluate the quality of care as well as appropriateness of placement, provided the information collected is designed with these ends in mind.", "PMID": 961947}
{"id": "pubmed23n0032_4786", "title": "Communication, compliance, and concordance between physicians and patients with prescribed medications.", "content": "Forty-six practicing physicians and 357 patients with diabetes mellitus or congestive heart failure were the subjects for this study, which focuses on the impact of medication regimen and doctor-patient communication in affecting patient medication-taking behavior and physician awareness of these behaviors. Four types of medication errors were defined: omissions, commissions, scheduling misconceptions and scheduling non-compliance. The average error rates were 19 per cent, 19 per cent, 17 per cent and 3 per cent, respectively. The combined average error was 58 per cent; scheduline non-compliance on the part of the patient was a minor component. Specific aspects of the medication regimen were associated with increased errors: (1) the more drugs involved between the doctor-patient pair, the greater the errors of omission and commission; and (2) the greater the complexity of the scheduling, the greater the errors of commission and scheduling misconceptions. If the patient did not know the function of all his drugs, errors of commission and scheduling misconception increased. Neither characteristics of patients nor the severity of disease were influential in determining the extent of medication errors. For patients with congestive heart failure, good communication of instructions and information from physician to patient was associated with low levels of all types of errors.", "contents": "Communication, compliance, and concordance between physicians and patients with prescribed medications. Forty-six practicing physicians and 357 patients with diabetes mellitus or congestive heart failure were the subjects for this study, which focuses on the impact of medication regimen and doctor-patient communication in affecting patient medication-taking behavior and physician awareness of these behaviors. Four types of medication errors were defined: omissions, commissions, scheduling misconceptions and scheduling non-compliance. The average error rates were 19 per cent, 19 per cent, 17 per cent and 3 per cent, respectively. The combined average error was 58 per cent; scheduline non-compliance on the part of the patient was a minor component. Specific aspects of the medication regimen were associated with increased errors: (1) the more drugs involved between the doctor-patient pair, the greater the errors of omission and commission; and (2) the greater the complexity of the scheduling, the greater the errors of commission and scheduling misconceptions. If the patient did not know the function of all his drugs, errors of commission and scheduling misconception increased. Neither characteristics of patients nor the severity of disease were influential in determining the extent of medication errors. For patients with congestive heart failure, good communication of instructions and information from physician to patient was associated with low levels of all types of errors.", "PMID": 961952}
{"id": "pubmed23n0032_4787", "title": "Racial difference in incidence of ABO hemolytic disease.", "content": "In this review of 7,464 consecutive infants born at North Carolina Memorial Hospital, hemolytic disease from ABO incompatibility was found to be two to three times as common in black infants as in white infants. The statistical significance of the difference remained high as more restrictive criteria for ABO hemolytic disease were applied. ABO disease, serious enough to cause an indirect serum bilirubin of 15 mg/100ml or higher, had an incidence in black newborns as great as the incidence of Rh hemolytic disease in whites. In contrast, the general prevalence and severity of hyperbilirubinemia was not found to be higher in black newborns than in white infants. The difference cannot be attributed to differences in the prevalence of ABO blood groups between the two races. Policies of early discharge of newborns could be affected by the finding that ABO erythroblastosis is two to three times as common in black infants as in white infants.", "contents": "Racial difference in incidence of ABO hemolytic disease. In this review of 7,464 consecutive infants born at North Carolina Memorial Hospital, hemolytic disease from ABO incompatibility was found to be two to three times as common in black infants as in white infants. The statistical significance of the difference remained high as more restrictive criteria for ABO hemolytic disease were applied. ABO disease, serious enough to cause an indirect serum bilirubin of 15 mg/100ml or higher, had an incidence in black newborns as great as the incidence of Rh hemolytic disease in whites. In contrast, the general prevalence and severity of hyperbilirubinemia was not found to be higher in black newborns than in white infants. The difference cannot be attributed to differences in the prevalence of ABO blood groups between the two races. Policies of early discharge of newborns could be affected by the finding that ABO erythroblastosis is two to three times as common in black infants as in white infants.", "PMID": 961953}
{"id": "pubmed23n0032_4788", "title": "Estimates of motor vehicle seat belt effectiveness and use: implications for occupant crash protection.", "content": "Estimates of the effectiveness of seat belts, when used, in reducing motor vehicle occupant deaths vary widely. A recently publicized claim by one analyst that seat belts reduce vehicle occupant deaths 70-80 per cent is based on studies found to contain fundamental systematic error. Deaths occur only 50 per cent less often to belted compared to nonbelted vehicle occupants in crashes, according to previously unanalyzed data from three U.S. states during recent years. New belt systems would be about 60 per cent effective with 100 per cent use. But surveys of observed belt use in 1975 U.S. cars indicate that two-thirds of drivers were not using belts. Prospects for widespread adoption and enforcement of belt use laws in the U.S. are not encouraging. Substantial reductions in fatal and other injuries would result from the adoption of requirements mandating automatic (passive) protection for front seat occupants in crashes with forward decelerations.", "contents": "Estimates of motor vehicle seat belt effectiveness and use: implications for occupant crash protection. Estimates of the effectiveness of seat belts, when used, in reducing motor vehicle occupant deaths vary widely. A recently publicized claim by one analyst that seat belts reduce vehicle occupant deaths 70-80 per cent is based on studies found to contain fundamental systematic error. Deaths occur only 50 per cent less often to belted compared to nonbelted vehicle occupants in crashes, according to previously unanalyzed data from three U.S. states during recent years. New belt systems would be about 60 per cent effective with 100 per cent use. But surveys of observed belt use in 1975 U.S. cars indicate that two-thirds of drivers were not using belts. Prospects for widespread adoption and enforcement of belt use laws in the U.S. are not encouraging. Substantial reductions in fatal and other injuries would result from the adoption of requirements mandating automatic (passive) protection for front seat occupants in crashes with forward decelerations.", "PMID": 961954}
{"id": "pubmed23n0032_4789", "title": "Dental visits by income and race in ten urban and two rural areas.", "content": "Household surveys in 12 low income areas found large differences in dental visit rates after control for income and race. The dental visit rate for Red Hook (NYC) exceeded the national rate whereas in seven of the areas the rate was below national averages by 40 per cent or more. The ranges in dental visit rate for low income Blacks was from two-thirds the national rate (in two areas of the South) to two to three times greater than the comparable national rate (in three areas of the Northeast). Lesser but nevertheless large variations among area dental visit rates existed for other race and income groups.", "contents": "Dental visits by income and race in ten urban and two rural areas. Household surveys in 12 low income areas found large differences in dental visit rates after control for income and race. The dental visit rate for Red Hook (NYC) exceeded the national rate whereas in seven of the areas the rate was below national averages by 40 per cent or more. The ranges in dental visit rate for low income Blacks was from two-thirds the national rate (in two areas of the South) to two to three times greater than the comparable national rate (in three areas of the Northeast). Lesser but nevertheless large variations among area dental visit rates existed for other race and income groups.", "PMID": 961956}
{"id": "pubmed23n0032_4790", "title": "Trends in intravenous drug abuse as reflected in national hepatitis reporting.", "content": "A procedure for obtaining an indicator of trends in illicit intravenous (I.V.) drug use-a form of drug use which is very harmful and difficult to measure-has been developed using national hepatitis surveillance data. Hepatitis reports are separated into two groups: one containing mostly cases related to transmission via I.V. drug use and the other containing cases related to transmission via personal contact and blood transfusion. The analysis of ten years of national hepatitis reporting (1966 to 1975) shows an almost ten-fold rise in drug-related hepatitis cases from 1966 to 1972. In the last three years the number of cases has declined but remains substantially greater than the pre-epidemic levels. The rise in I.V. drug-related cases began in the 1960's among minority groups living in the center cities of the East and West Coasts and spread during the 1970's into the suburbs of these cities and into metropolitan areas throughout the United States. Limitations of this indicator of I.V. drug use relate to the characteristics of the surveillance system and to the underlying relationship of hepatitis to I.V. drug use.", "contents": "Trends in intravenous drug abuse as reflected in national hepatitis reporting. A procedure for obtaining an indicator of trends in illicit intravenous (I.V.) drug use-a form of drug use which is very harmful and difficult to measure-has been developed using national hepatitis surveillance data. Hepatitis reports are separated into two groups: one containing mostly cases related to transmission via I.V. drug use and the other containing cases related to transmission via personal contact and blood transfusion. The analysis of ten years of national hepatitis reporting (1966 to 1975) shows an almost ten-fold rise in drug-related hepatitis cases from 1966 to 1972. In the last three years the number of cases has declined but remains substantially greater than the pre-epidemic levels. The rise in I.V. drug-related cases began in the 1960's among minority groups living in the center cities of the East and West Coasts and spread during the 1970's into the suburbs of these cities and into metropolitan areas throughout the United States. Limitations of this indicator of I.V. drug use relate to the characteristics of the surveillance system and to the underlying relationship of hepatitis to I.V. drug use.", "PMID": 961955}
{"id": "pubmed23n0032_4791", "title": "Brucella canis infectivity rates in stray and pet dog populations.", "content": "A serological survey of 200 healthy, mature dogs was made to determine Brucella canis infectivity rates. The 9 per cent rate reported in the stray dogs was significantly higher (0.05 less than P greater than 0.025) than the 1 per cent rate found in pet dogs. These rates coupled with a predictably growing stray dog population have patent zoonotic implications.", "contents": "Brucella canis infectivity rates in stray and pet dog populations. A serological survey of 200 healthy, mature dogs was made to determine Brucella canis infectivity rates. The 9 per cent rate reported in the stray dogs was significantly higher (0.05 less than P greater than 0.025) than the 1 per cent rate found in pet dogs. These rates coupled with a predictably growing stray dog population have patent zoonotic implications.", "PMID": 961957}
{"id": "pubmed23n0032_4792", "title": "Suprascapular neuropathy in the differential diagnosis of rotator cuff injuries.", "content": "Four cases of suprascapular neuropathy treated by nonoperative means have been presented. Complete recovery occurred in all four. The anatomy of the suprascapular nerve and probable mechanisms of injury secondary to traction have been discussed. The importance of electromyography in diagnosis has been stressed. Longer periods of nonoperative treatment are recommended here than by previous authors. Since the lesion is felt not to be an entrapment phenomenon but, rather, a traction injury, operative treatment should consist of release of the nerve at the notch to reduce the possibility of further traction injury, and a neurolysis should be done as well.", "contents": "Suprascapular neuropathy in the differential diagnosis of rotator cuff injuries. Four cases of suprascapular neuropathy treated by nonoperative means have been presented. Complete recovery occurred in all four. The anatomy of the suprascapular nerve and probable mechanisms of injury secondary to traction have been discussed. The importance of electromyography in diagnosis has been stressed. Longer periods of nonoperative treatment are recommended here than by previous authors. Since the lesion is felt not to be an entrapment phenomenon but, rather, a traction injury, operative treatment should consist of release of the nerve at the notch to reduce the possibility of further traction injury, and a neurolysis should be done as well.", "PMID": 961967}
{"id": "pubmed23n0032_4793", "title": "Achilles tendonitis in runners: a report of five cases.", "content": "Achilles tendonitis is a significant disability in the runner and may often cause runners to lose an entire season of competition. The exact pathophysiology of the problem is uncertain. However, it is the belief of the authors that Achilles tendonitis in the runner often represents a microscopic tear in the tendon which, if not adequately treated, may lead to a macroscopic tear. We recommend surgical treatment in those patients with chronic symptoms who have not responded to conservative measures.", "contents": "Achilles tendonitis in runners: a report of five cases. Achilles tendonitis is a significant disability in the runner and may often cause runners to lose an entire season of competition. The exact pathophysiology of the problem is uncertain. However, it is the belief of the authors that Achilles tendonitis in the runner often represents a microscopic tear in the tendon which, if not adequately treated, may lead to a macroscopic tear. We recommend surgical treatment in those patients with chronic symptoms who have not responded to conservative measures.", "PMID": 961968}
{"id": "pubmed23n0032_4794", "title": "An evaluation of the Elmslie-Trillat procedure for management of patellar dislocations and subluxations: a preliminary report.", "content": "The Elmslie-Trillat procedure involves lateral retinacular release, medial capsular reefing, and medial transposition of the anterior tibial tubercle hinged on a distal periosteal attachment. It is an easily performed procedure and is an excellent method for realignment of the extensor mechanism in cases of dislocation and subluxation of the patella. In our series of 52 cases with 6-24 months postsurgical evaluation, 46 cases, or 88 percent were rated as good or excellent. It must be emphasized that this technique is used only if the epiphyseal plates are closed.", "contents": "An evaluation of the Elmslie-Trillat procedure for management of patellar dislocations and subluxations: a preliminary report. The Elmslie-Trillat procedure involves lateral retinacular release, medial capsular reefing, and medial transposition of the anterior tibial tubercle hinged on a distal periosteal attachment. It is an easily performed procedure and is an excellent method for realignment of the extensor mechanism in cases of dislocation and subluxation of the patella. In our series of 52 cases with 6-24 months postsurgical evaluation, 46 cases, or 88 percent were rated as good or excellent. It must be emphasized that this technique is used only if the epiphyseal plates are closed.", "PMID": 961970}
{"id": "pubmed23n0032_4795", "title": "A dynamic method of repair for acute and chronic acromioclavicular disruption.", "content": "The authors have presented a surgical procedure for disruption of the acromioclavicular joint with or without fracture of the lateral end of the clavicle. The results of the dynamic repairs have been excellent with restoration of stability and normal strength in all cases. The majority of this group were vigorous young high school college and professional athletes prior to their injury and all were returned to their previous activities.", "contents": "A dynamic method of repair for acute and chronic acromioclavicular disruption. The authors have presented a surgical procedure for disruption of the acromioclavicular joint with or without fracture of the lateral end of the clavicle. The results of the dynamic repairs have been excellent with restoration of stability and normal strength in all cases. The majority of this group were vigorous young high school college and professional athletes prior to their injury and all were returned to their previous activities.", "PMID": 961969}
{"id": "pubmed23n0032_4796", "title": "Therapeutic evaluation of oxantel pamoate (1, 4, 5, 6-tetrahydro-1-methyl-2-[trans-3-hydroxystyryl] pyrimidine pamoate) in severe Trichuris trichiura infection.", "content": "The efficacy of oxantel pamoate (1, 4, 5, 6-tetrahydro-2-[trans-3-hydroxystyryl]-1-methyl pyrimidine pamoate) was evaluated in 25 children with severe Trichuris infection. The presence of chronic dysentery and sigmoidoscopic demonstration of whipworms in the intestinal mucosa were the criteria for inclusion in the trial. Most of these patients had severe anemia, rectal prolapse, digital clubbing, hypoproteinemia, and growth retardation. There were a high incidence of concomitant parasitism with other intestinal helminths and with Entamoeba histolytica and Giardia lamblia. Sigmoidoscopic grading of Trichuris load and egg count in the feces were carried out prior to treatment and 2 days after each course of oxantel therapy. Oxantel was administered at a dose of 10 mg/kg body weight twice daily for a 3-day course. Satisfactory response, as judged by relief of dysentery and absence of whipworms from the mucosa at sigmoidoscopy, was achieved in 17 patients after the first course and in the remaining 8 patients after a second course of oxantel. The drug was well tolerated and no side effects were noted during or after treatment. It is concluded that oxantel is a safe and effective anthelmintic for severe clinical trichuriasis.", "contents": "Therapeutic evaluation of oxantel pamoate (1, 4, 5, 6-tetrahydro-1-methyl-2-[trans-3-hydroxystyryl] pyrimidine pamoate) in severe Trichuris trichiura infection. The efficacy of oxantel pamoate (1, 4, 5, 6-tetrahydro-2-[trans-3-hydroxystyryl]-1-methyl pyrimidine pamoate) was evaluated in 25 children with severe Trichuris infection. The presence of chronic dysentery and sigmoidoscopic demonstration of whipworms in the intestinal mucosa were the criteria for inclusion in the trial. Most of these patients had severe anemia, rectal prolapse, digital clubbing, hypoproteinemia, and growth retardation. There were a high incidence of concomitant parasitism with other intestinal helminths and with Entamoeba histolytica and Giardia lamblia. Sigmoidoscopic grading of Trichuris load and egg count in the feces were carried out prior to treatment and 2 days after each course of oxantel therapy. Oxantel was administered at a dose of 10 mg/kg body weight twice daily for a 3-day course. Satisfactory response, as judged by relief of dysentery and absence of whipworms from the mucosa at sigmoidoscopy, was achieved in 17 patients after the first course and in the remaining 8 patients after a second course of oxantel. The drug was well tolerated and no side effects were noted during or after treatment. It is concluded that oxantel is a safe and effective anthelmintic for severe clinical trichuriasis.", "PMID": 961973}
{"id": "pubmed23n0032_4797", "title": "Mebendazole in treatment of severe Trichuris trichiura infection in Malaysian children.", "content": "Children with severe Trichuris trichiura infection pose significant social, clinical, and therapeutic problems in Malaysia. Thirty such children were investigated, and mebendazole was found to be safe and effective in the treatment of severe trichuriasis but it had to be given for a longer period than currently recommended. A poor correlation was found between egg load and worm burden in these children. Direct visualization of the rectal and colonic mucosa was the most reliable method of assessing severity in untreated cases, and response to treatment. The eggs of Trichuris that had been exposed to mebendazole were morphologically altered and not viable when incubated. This may be of considerable epidemiological importance.", "contents": "Mebendazole in treatment of severe Trichuris trichiura infection in Malaysian children. Children with severe Trichuris trichiura infection pose significant social, clinical, and therapeutic problems in Malaysia. Thirty such children were investigated, and mebendazole was found to be safe and effective in the treatment of severe trichuriasis but it had to be given for a longer period than currently recommended. A poor correlation was found between egg load and worm burden in these children. Direct visualization of the rectal and colonic mucosa was the most reliable method of assessing severity in untreated cases, and response to treatment. The eggs of Trichuris that had been exposed to mebendazole were morphologically altered and not viable when incubated. This may be of considerable epidemiological importance.", "PMID": 961974}
{"id": "pubmed23n0032_4798", "title": "Effect of inoculum size and length of infection on the distribution of Toxocara canis larvae in the mouse.", "content": "The effect of inoculum size and time on the distribution of Toxocara canis larvae in the mouse was investigated by recovering larvae from various body regions 7, 14, 28, and 56 days after administration of either 200, 600 or 1,000 infective eggs to groups of ten male mice. An analysis of variance of larval recoveries from the carcass, liver, brain and cardiopulmonary system suggests that inoculum size was a significant factor determining the proportional recovery for each of these sites. Length of infection was significant in relation to numbers of larvae in the anterior carcass, genitourinary system, brain and heart plus lungs, while length of infection and inoculum size acting in concert influenced the numbers of larvae recovered from the carcass, liver, brain, heart and lungs. Crowding effects, manifested as altered dispersion rates, were seen in the heavier infections.", "contents": "Effect of inoculum size and length of infection on the distribution of Toxocara canis larvae in the mouse. The effect of inoculum size and time on the distribution of Toxocara canis larvae in the mouse was investigated by recovering larvae from various body regions 7, 14, 28, and 56 days after administration of either 200, 600 or 1,000 infective eggs to groups of ten male mice. An analysis of variance of larval recoveries from the carcass, liver, brain and cardiopulmonary system suggests that inoculum size was a significant factor determining the proportional recovery for each of these sites. Length of infection was significant in relation to numbers of larvae in the anterior carcass, genitourinary system, brain and heart plus lungs, while length of infection and inoculum size acting in concert influenced the numbers of larvae recovered from the carcass, liver, brain, heart and lungs. Crowding effects, manifested as altered dispersion rates, were seen in the heavier infections.", "PMID": 961975}
{"id": "pubmed23n0032_4799", "title": "Immunoglobulin E: raised levels in sera and pleural exudates of patients with paragonimiasis.", "content": "Serum IgE concentrations of patients with paragonimiasis were determined by a radioimmunosorbent test. The mean concentration was 3,462.3 IU/ml in a group of 13 cases of paragonimiasis miyazakii in which patients showed clinical symptoms and/or positive immunological diagnostic tests, and 1,026.6 IU/ml in a control group of 13 individuals who had eaten uncooked freshwater crabs, Potamon dehaani, but had been found to be free from the infection. Moreover, the IgE level of the pleural exudates obtained from four patients with paragonimiasis miyazakii on the day of bleeding or within several days after was significantly higher than that of their sera, ranging between 4,200 IU/ml and 10,000 IU/ml. This was true also in a case of paragonimiasis westermani. Sera and pleural exudates of patients with both forms of paragonimiasis were applied to immunosorbent columns of Sepharose 4B beads coupled with saline extracts of Paragonimus miyazakii, P. weetermani, or P. ohirai. IgE eluted from the corresponding column was considered to be specific, being around 5% to 10% of the total IgE.", "contents": "Immunoglobulin E: raised levels in sera and pleural exudates of patients with paragonimiasis. Serum IgE concentrations of patients with paragonimiasis were determined by a radioimmunosorbent test. The mean concentration was 3,462.3 IU/ml in a group of 13 cases of paragonimiasis miyazakii in which patients showed clinical symptoms and/or positive immunological diagnostic tests, and 1,026.6 IU/ml in a control group of 13 individuals who had eaten uncooked freshwater crabs, Potamon dehaani, but had been found to be free from the infection. Moreover, the IgE level of the pleural exudates obtained from four patients with paragonimiasis miyazakii on the day of bleeding or within several days after was significantly higher than that of their sera, ranging between 4,200 IU/ml and 10,000 IU/ml. This was true also in a case of paragonimiasis westermani. Sera and pleural exudates of patients with both forms of paragonimiasis were applied to immunosorbent columns of Sepharose 4B beads coupled with saline extracts of Paragonimus miyazakii, P. weetermani, or P. ohirai. IgE eluted from the corresponding column was considered to be specific, being around 5% to 10% of the total IgE.", "PMID": 961976}
{"id": "pubmed23n0032_4800", "title": "Schistosomiasis in the Gorgol Valley of Mauritania.", "content": "Schistosomiasis may be increasing in West Africa due to the development of water-resource projects which were given a strong impetus by the disastrous Sahelian drought. This report concerns the existing epidemiology of schistosomiasis in West Africa for the Fourth Region of Mauritania, along the Gorgol River. In this area two reservoirs are proposed, to supply a new rice irrigation system. During May and November 1974, field surveys were made to determine the prevalence and intensity of schistosomiasis, and the relevant species of snails. The surveys were made as a basis for designing the irrigation systems to minimize transmission. The only snails found in the area were two species of Bulinus; therefore, the human population was tested for Schistosoma haematobium. A high prevalence of schistosomiasis was found in M'Bout, the town nearest the proposed reservoir at Foum Gleita. Prevalence rates were lowest in Kaedi near the confluence of the Gorgol and Senegal rivers. During the period between the first and second survey the incidence rate among children in M'Bout became very high (68%), probably because of increased water contact related to the rains that broke the long drought in August 1974.", "contents": "Schistosomiasis in the Gorgol Valley of Mauritania. Schistosomiasis may be increasing in West Africa due to the development of water-resource projects which were given a strong impetus by the disastrous Sahelian drought. This report concerns the existing epidemiology of schistosomiasis in West Africa for the Fourth Region of Mauritania, along the Gorgol River. In this area two reservoirs are proposed, to supply a new rice irrigation system. During May and November 1974, field surveys were made to determine the prevalence and intensity of schistosomiasis, and the relevant species of snails. The surveys were made as a basis for designing the irrigation systems to minimize transmission. The only snails found in the area were two species of Bulinus; therefore, the human population was tested for Schistosoma haematobium. A high prevalence of schistosomiasis was found in M'Bout, the town nearest the proposed reservoir at Foum Gleita. Prevalence rates were lowest in Kaedi near the confluence of the Gorgol and Senegal rivers. During the period between the first and second survey the incidence rate among children in M'Bout became very high (68%), probably because of increased water contact related to the rains that broke the long drought in August 1974.", "PMID": 961977}
{"id": "pubmed23n0032_4801", "title": "Tissue degradation of calcific Schistosoma haematobium eggs.", "content": "Calcific eggs of Schistosoma haematobium were separated from an inactive sandy patch in a cystectomy specimen from an Egyptian farmer; the purified egg suspension was injected into tail veins of inbred mice, some of which were killed immediately and some at intervals of 1, 2, 11, 14, 30, 90, 120, 180, and 365 days. Lungs were examined by tissue digestion and histologic analysis. Calific egg burdens decayed in an exponential manner (eggs/g of lung tissue = 284.9e-0.001x where e = natural log and x = time in days), with a loss of 5,610 eggs/g of lung tissue, i.e., 98% reduction over the year of the experiment. Histologic examination revealed that calcific S. haematobium eggs incited a typical granulomatous reaction as they decalcified.  Compared with viable egg granulomata, the response to calcific eggs was accelerated in onset, of lesser magnitude but persisted longer. Small numbers of calcific eggs persisted for at least 1 year after injection of either viable or calcific eggs. It is concluded that despite the apparent lack of tissue reaction to calcific eggs in chronic bladder lesions, these eggs are not antigenically exhausted foreign bodies. Moreover, it is shown that calcific eggs are degraded in significant numbers by mammalian tissue.", "contents": "Tissue degradation of calcific Schistosoma haematobium eggs. Calcific eggs of Schistosoma haematobium were separated from an inactive sandy patch in a cystectomy specimen from an Egyptian farmer; the purified egg suspension was injected into tail veins of inbred mice, some of which were killed immediately and some at intervals of 1, 2, 11, 14, 30, 90, 120, 180, and 365 days. Lungs were examined by tissue digestion and histologic analysis. Calific egg burdens decayed in an exponential manner (eggs/g of lung tissue = 284.9e-0.001x where e = natural log and x = time in days), with a loss of 5,610 eggs/g of lung tissue, i.e., 98% reduction over the year of the experiment. Histologic examination revealed that calcific S. haematobium eggs incited a typical granulomatous reaction as they decalcified.  Compared with viable egg granulomata, the response to calcific eggs was accelerated in onset, of lesser magnitude but persisted longer. Small numbers of calcific eggs persisted for at least 1 year after injection of either viable or calcific eggs. It is concluded that despite the apparent lack of tissue reaction to calcific eggs in chronic bladder lesions, these eggs are not antigenically exhausted foreign bodies. Moreover, it is shown that calcific eggs are degraded in significant numbers by mammalian tissue.", "PMID": 961978}
{"id": "pubmed23n0032_4802", "title": "Hycanthone dose-response in treatment of schistosomiasis mansoni in St. Lucia.", "content": "Clinical trials of hycanthone (single intramuscular dose) were undertaken in schistosomiasis mansoni patients in St. Lucia at five dose levels: 3.0, 2.5, 2.0, 1.5, and 1.0 mg/kg body weight. The most common side effect, vomiting, decreased in frequency from 51% at the highest dose to 3% at the lowest; minor side effects showed a similar trend. Three fecal specimens were examined before and at 6 months after treatment by qualitative, quantitative, and hatching techniques. All dose levels caused reductions in egg excretion of 89 to 98%. Rates of cure (absence of eggs by all three methods) according to dose (descending), pretreatment egg output (0-19, 20-49, 50-399, 400+ eggs/ml feces), and age (0-7, 8-14, 15-29, 30+ years) were analyzed to estimate the effect of each variable if the others had been constant. For dose, the standardized percentage success rates were 53.9%, 62.0%, 51.2% 54.0%, and 27.4%; for egg output, 67.0%, 51.8%, 43.2%, and 21.7%; and for age, 25.2%, 34.5%, 59.3% and 57.4%. Logit regression analysis shows a significant difference in cure rate (a) between the lowest dose and all others, among which latter there was no difference, (b) between patients excreting 0 to 49 eggs/ml before treatment and those excreting 50+ eggs/ml, and (c) between the age groups 0 to 14 and 15+ years. All dose levels caused some regression in enlargement of liver or spleen. A dose of 1.5 to 2.0 mg/kg body weight is considered to be as effective as one of 3.0 mg/kg and more acceptable for a control program because of the marked reduction in side effects.", "contents": "Hycanthone dose-response in treatment of schistosomiasis mansoni in St. Lucia. Clinical trials of hycanthone (single intramuscular dose) were undertaken in schistosomiasis mansoni patients in St. Lucia at five dose levels: 3.0, 2.5, 2.0, 1.5, and 1.0 mg/kg body weight. The most common side effect, vomiting, decreased in frequency from 51% at the highest dose to 3% at the lowest; minor side effects showed a similar trend. Three fecal specimens were examined before and at 6 months after treatment by qualitative, quantitative, and hatching techniques. All dose levels caused reductions in egg excretion of 89 to 98%. Rates of cure (absence of eggs by all three methods) according to dose (descending), pretreatment egg output (0-19, 20-49, 50-399, 400+ eggs/ml feces), and age (0-7, 8-14, 15-29, 30+ years) were analyzed to estimate the effect of each variable if the others had been constant. For dose, the standardized percentage success rates were 53.9%, 62.0%, 51.2% 54.0%, and 27.4%; for egg output, 67.0%, 51.8%, 43.2%, and 21.7%; and for age, 25.2%, 34.5%, 59.3% and 57.4%. Logit regression analysis shows a significant difference in cure rate (a) between the lowest dose and all others, among which latter there was no difference, (b) between patients excreting 0 to 49 eggs/ml before treatment and those excreting 50+ eggs/ml, and (c) between the age groups 0 to 14 and 15+ years. All dose levels caused some regression in enlargement of liver or spleen. A dose of 1.5 to 2.0 mg/kg body weight is considered to be as effective as one of 3.0 mg/kg and more acceptable for a control program because of the marked reduction in side effects.", "PMID": 961979}
{"id": "pubmed23n0032_4803", "title": "Relation of intensity of infection to disease in hamsters with acute schistosomiasis mansoni.", "content": "Groups of young hamsters were exposed to 3, 20, 40, 80, or 160 cercariae. A highly significant correlation was observed between the number of cercariae, worm burdens, and liver and fecal egg counts. The most heavily infected animals were the first to lose weight and die. Hamsters exposed to 20 or more cercariae and harboring a mean of 4.2 or more worm pairs developed significant hepatosplenic disease by 10 weeks after infection as assessed by hepatomegaly, splenomegaly and the development of portal hypertension. Lightly infected animals with single worm pairs did not develop significant disease.", "contents": "Relation of intensity of infection to disease in hamsters with acute schistosomiasis mansoni. Groups of young hamsters were exposed to 3, 20, 40, 80, or 160 cercariae. A highly significant correlation was observed between the number of cercariae, worm burdens, and liver and fecal egg counts. The most heavily infected animals were the first to lose weight and die. Hamsters exposed to 20 or more cercariae and harboring a mean of 4.2 or more worm pairs developed significant hepatosplenic disease by 10 weeks after infection as assessed by hepatomegaly, splenomegaly and the development of portal hypertension. Lightly infected animals with single worm pairs did not develop significant disease.", "PMID": 961980}
{"id": "pubmed23n0032_4804", "title": "Anti-liver antibody in rabbits infected with Schistosoma japonicum.", "content": "Anti-liver antibody has been demonstrated in the sera of Schistosoma japonicum-infected rabbits. By the 8th week after exposure to cercariae, perceptible levels of autoantibody were present and appeared to be direct predominantly determinants located in the cell membrane of the hepatocyte.", "contents": "Anti-liver antibody in rabbits infected with Schistosoma japonicum. Anti-liver antibody has been demonstrated in the sera of Schistosoma japonicum-infected rabbits. By the 8th week after exposure to cercariae, perceptible levels of autoantibody were present and appeared to be direct predominantly determinants located in the cell membrane of the hepatocyte.", "PMID": 961981}
{"id": "pubmed23n0032_4805", "title": "Evaluation of immunodiagnostic techniques for the detection of human hydatid cyst carriers in field studies.", "content": "In a study of 662 sera from a hydatidosis endemic area, the indirect hemagglutination test based on a minimal nonspecificity criterion of positivity and the latex agglutination (LA) test were found to be suitable screening techniques for the detection of sera positive to the arc 5, diagnostic of hydatid infection. The lower nonspecificity of the LA test, its greater simplicity and its excellent correlation with the immunoelectrophoresis test suggest that it is the choice screening technique for use in field surveys or seroepidemiologic studies of hydatid disease. The advantages and limitations of this serologic approach for the detection of human hydatid cyst carriers in field studies are discussed.", "contents": "Evaluation of immunodiagnostic techniques for the detection of human hydatid cyst carriers in field studies. In a study of 662 sera from a hydatidosis endemic area, the indirect hemagglutination test based on a minimal nonspecificity criterion of positivity and the latex agglutination (LA) test were found to be suitable screening techniques for the detection of sera positive to the arc 5, diagnostic of hydatid infection. The lower nonspecificity of the LA test, its greater simplicity and its excellent correlation with the immunoelectrophoresis test suggest that it is the choice screening technique for use in field surveys or seroepidemiologic studies of hydatid disease. The advantages and limitations of this serologic approach for the detection of human hydatid cyst carriers in field studies are discussed.", "PMID": 961982}
{"id": "pubmed23n0032_4806", "title": "Bubonic plague from direct exposure to a naturally infected wild coyote.", "content": "An 11-year-old boy developed axillary bubonic plague and plague meningitis 3 days after skinning a dead coyote near Albuquerque, New Mexico. The coyoto carcass was recovered 10 days later, and Yersinia pestis was isolated from spleen and marrow of the animal. This is the first report of human plague from exposure to a coyote. A review of experimental and epidemiologic studies suggests that severe plague infection in members of the family Canidae is unusual, and that the risk of acquiring plague from direct contact with coyote tissues is minimal. Nevertheless, certain precautions are outlined for persons working with wild coyotes.", "contents": "Bubonic plague from direct exposure to a naturally infected wild coyote. An 11-year-old boy developed axillary bubonic plague and plague meningitis 3 days after skinning a dead coyote near Albuquerque, New Mexico. The coyoto carcass was recovered 10 days later, and Yersinia pestis was isolated from spleen and marrow of the animal. This is the first report of human plague from exposure to a coyote. A review of experimental and epidemiologic studies suggests that severe plague infection in members of the family Canidae is unusual, and that the risk of acquiring plague from direct contact with coyote tissues is minimal. Nevertheless, certain precautions are outlined for persons working with wild coyotes.", "PMID": 961983}
{"id": "pubmed23n0032_4807", "title": "Experimental alimentary infection of anole lizards (Anolis carolinensis) with Mycobacterium ulcerans.", "content": "Pathogenic Mycobacterium ulcerans were recovered from the stool of anole lizards up to 11 days after inoculation by stomach tube. M. ulcerans was isolated from the liver of 3 of 20 lizards and acid fast bacteria were seen in the mucosa of intrahepatic bile ducts in 2 of these 10 weeks post-inoculation. These results provide equivocal support for our hypothesis that herpetofauna are a reservoir of infection with M. ulcerans.", "contents": "Experimental alimentary infection of anole lizards (Anolis carolinensis) with Mycobacterium ulcerans. Pathogenic Mycobacterium ulcerans were recovered from the stool of anole lizards up to 11 days after inoculation by stomach tube. M. ulcerans was isolated from the liver of 3 of 20 lizards and acid fast bacteria were seen in the mucosa of intrahepatic bile ducts in 2 of these 10 weeks post-inoculation. These results provide equivocal support for our hypothesis that herpetofauna are a reservoir of infection with M. ulcerans.", "PMID": 961984}
{"id": "pubmed23n0032_4808", "title": "Sickle cell trait, hemoglobin C trait, and Burkitt's lymphoma.", "content": "In a controlled study in Ghana, the hemoglobin electrophoretic pattern in 112 patients with Burkitt's lymphoma was compared to that of their nearest neighbor controls of the same age, sex, and tribe, as well as their sibling controls. Analysis of the data obtained did not show any statistically significant protective advantage for sickle cell trait against Burkitt's lymphoma. Hemoglobin C trait appeared to offer a slight protective advantage (p less than 0.1), but this did not reach statistical significance. These results do not disprove the malaria co-factor hypothesis in the etiology of 0urkitt's lymphoma, but deprive it of an additional indirect evidence in its favor.", "contents": "Sickle cell trait, hemoglobin C trait, and Burkitt's lymphoma. In a controlled study in Ghana, the hemoglobin electrophoretic pattern in 112 patients with Burkitt's lymphoma was compared to that of their nearest neighbor controls of the same age, sex, and tribe, as well as their sibling controls. Analysis of the data obtained did not show any statistically significant protective advantage for sickle cell trait against Burkitt's lymphoma. Hemoglobin C trait appeared to offer a slight protective advantage (p less than 0.1), but this did not reach statistical significance. These results do not disprove the malaria co-factor hypothesis in the etiology of 0urkitt's lymphoma, but deprive it of an additional indirect evidence in its favor.", "PMID": 961985}
{"id": "pubmed23n0032_4809", "title": "Relation of mosquito density to bird: mammal feeding ratios of Culex tarsalis in stable traps.", "content": "Stable traps, each baited with a jackrabbit and either a chicken or a pheasant, collected more than 21,000 mosquitoes in the Sacramento Valley, California, in 1972 and 1973. The focus of interest was the feeding behavior of Culex tarsalis, a primary vector of encephalitis viruses. Generally, feeding success was less and feeding rates on the jackrabbit were greater when larger numbers of mosquitoes were collected, or when a bird was exposed that was less receptive to mosquito feeding. Greater feeding rates on the jackrabbit apparently resulted from decreased feeding on the avian host and from diversion of mosquitoes to the jackrabbit. Figures are given that show how changes in feeding rates on birds affect the probabilities that a mosquito that feeds twice would feel both times on a bird, or on a bird and then on a mammal. Relatively small collections of nine mosquito species other than C. tarsalis revealed distinct species differences in feeding behavior. Aedes malanimon showed a preference for jackrabbit, which is relevant to its role as a vector of western equine encephalitis and California encephalitis viruses.", "contents": "Relation of mosquito density to bird: mammal feeding ratios of Culex tarsalis in stable traps. Stable traps, each baited with a jackrabbit and either a chicken or a pheasant, collected more than 21,000 mosquitoes in the Sacramento Valley, California, in 1972 and 1973. The focus of interest was the feeding behavior of Culex tarsalis, a primary vector of encephalitis viruses. Generally, feeding success was less and feeding rates on the jackrabbit were greater when larger numbers of mosquitoes were collected, or when a bird was exposed that was less receptive to mosquito feeding. Greater feeding rates on the jackrabbit apparently resulted from decreased feeding on the avian host and from diversion of mosquitoes to the jackrabbit. Figures are given that show how changes in feeding rates on birds affect the probabilities that a mosquito that feeds twice would feel both times on a bird, or on a bird and then on a mammal. Relatively small collections of nine mosquito species other than C. tarsalis revealed distinct species differences in feeding behavior. Aedes malanimon showed a preference for jackrabbit, which is relevant to its role as a vector of western equine encephalitis and California encephalitis viruses.", "PMID": 961987}
{"id": "pubmed23n0032_4810", "title": "Autopsy analysis of disease frequency in Kinshasa, Republic of Zaire.", "content": "Between July 1973 and December 1974, 304 autopsies were done at Hopital Mama Yemo, Kinshasa, Republic of Zaire. The causes of death and sudden death were tabulated and analyzed. The frequency of various diseases and groups of diseases among all the individuals and a large subset of individuals who died traumatically are tabulated and discussed. The present report is the most quantitative, albeit only, current estimate of mortality and prevalence of diseases in Zaire.", "contents": "Autopsy analysis of disease frequency in Kinshasa, Republic of Zaire. Between July 1973 and December 1974, 304 autopsies were done at Hopital Mama Yemo, Kinshasa, Republic of Zaire. The causes of death and sudden death were tabulated and analyzed. The frequency of various diseases and groups of diseases among all the individuals and a large subset of individuals who died traumatically are tabulated and discussed. The present report is the most quantitative, albeit only, current estimate of mortality and prevalence of diseases in Zaire.", "PMID": 961986}
{"id": "pubmed23n0032_4811", "title": "Brugia malayi in a cervical smear.", "content": "A microfilaria, identified as that of Brugia malayi, was found in a routine cervical smear from a healthy, 38-year-old female Malaysian aborigine.", "contents": "Brugia malayi in a cervical smear. A microfilaria, identified as that of Brugia malayi, was found in a routine cervical smear from a healthy, 38-year-old female Malaysian aborigine.", "PMID": 961988}
{"id": "pubmed23n0032_4812", "title": "Evaluation of the slide-latex agglutination and intradermal (Casoni) tests for echinococcosis.", "content": "Fifty-one patients with surgically proven hydatid disease were examined with the intradermal (ID) (Casoni) and latex-agglutination (LA) tests. Before surgery, results of the former test were 80.4% positive and of the latter were 82.3%. For 105 persons who were diagnosed free of hydatid cysts by surgical, clinical and serological observations, 6.7% were positive with the ID test and 4.8% with the LA test. The close agreement between results obtained with the LA and ID tests in this selected group of patients suggests that the LA test merits further evaluation as a diagnostic tool.", "contents": "Evaluation of the slide-latex agglutination and intradermal (Casoni) tests for echinococcosis. Fifty-one patients with surgically proven hydatid disease were examined with the intradermal (ID) (Casoni) and latex-agglutination (LA) tests. Before surgery, results of the former test were 80.4% positive and of the latter were 82.3%. For 105 persons who were diagnosed free of hydatid cysts by surgical, clinical and serological observations, 6.7% were positive with the ID test and 4.8% with the LA test. The close agreement between results obtained with the LA and ID tests in this selected group of patients suggests that the LA test merits further evaluation as a diagnostic tool.", "PMID": 961989}
{"id": "pubmed23n0032_4813", "title": "Effects of Ascaris infection of nutritional status in children.", "content": "The nutritional status of 30 children infected with Ascaris lumbricoides was compared with that of 30 uninfected controls matched for age, race, sex, and family income. Statistically significant evidence of an adverse effect of ascariasis on serum albumin levels and plasma vitamin C levels was found, but no child had inadequate levels of these nutrients. Suggestive evidence of an adverse effect of the infection on weight for height and on riboflavin nutriture was also found. There were no significant differences between infected and control children with respect to seven other laboratory measurements.", "contents": "Effects of Ascaris infection of nutritional status in children. The nutritional status of 30 children infected with Ascaris lumbricoides was compared with that of 30 uninfected controls matched for age, race, sex, and family income. Statistically significant evidence of an adverse effect of ascariasis on serum albumin levels and plasma vitamin C levels was found, but no child had inadequate levels of these nutrients. Suggestive evidence of an adverse effect of the infection on weight for height and on riboflavin nutriture was also found. There were no significant differences between infected and control children with respect to seven other laboratory measurements.", "PMID": 961990}
{"id": "pubmed23n0032_4814", "title": "Anisakid larva from the throat of an Alaskan Eskimo.", "content": "An anisakid nematode, identified as Phocanema sp. fourth-stage larva, was \"pulled\" from the throat of a 46-year-old Alaskan Eskimo. The nematode is described and illustrated.", "contents": "Anisakid larva from the throat of an Alaskan Eskimo. An anisakid nematode, identified as Phocanema sp. fourth-stage larva, was \"pulled\" from the throat of a 46-year-old Alaskan Eskimo. The nematode is described and illustrated.", "PMID": 961991}
{"id": "pubmed23n0032_4815", "title": "Strongyloides fulleborni-like infections in man in Papua New Guinea.", "content": "During the course of a survey of intestinal parasites among the inhabitants of villages along the Fly River in the Kiunga region of Papua New Guinea, eggs of a Strongyloides species were found in the feces of several persons. In subsequent studies, 93 (17.8%) of 520 persons examined from five villages were found to be infected with this parasite. The examination of parasitic and free-living stages of the worm revealed that it is very similar to S. fulleborni, a parasite of monkeys, baboons and apes in Africa and Asia, although a definitive identification could not be made with the material available. Since non-human primates have apparently never inhabited New Guinea, the origin of these S. fulleborni-like infections is unknown.", "contents": "Strongyloides fulleborni-like infections in man in Papua New Guinea. During the course of a survey of intestinal parasites among the inhabitants of villages along the Fly River in the Kiunga region of Papua New Guinea, eggs of a Strongyloides species were found in the feces of several persons. In subsequent studies, 93 (17.8%) of 520 persons examined from five villages were found to be infected with this parasite. The examination of parasitic and free-living stages of the worm revealed that it is very similar to S. fulleborni, a parasite of monkeys, baboons and apes in Africa and Asia, although a definitive identification could not be made with the material available. Since non-human primates have apparently never inhabited New Guinea, the origin of these S. fulleborni-like infections is unknown.", "PMID": 961992}
{"id": "pubmed23n0032_4816", "title": "Serum glycoproteins in schistosomiasis.", "content": "Serum glycoprotein electrophoretic fractions as well as protein bound hexose and seromucoid levels were measured in 21 male Egyptian patients before and after treatment for schistosomiasis, and in 8 normal controls. Results indicate that alpha1 and gamma-glycoprotein were significantly higher while beta-glycoprotein was lower in bilharzial patients than in controls. Protein-bound hexose and seromucoid levels were also significantly elevated in the patients. After treatment, the levels of these fractions approached those of the controls.", "contents": "Serum glycoproteins in schistosomiasis. Serum glycoprotein electrophoretic fractions as well as protein bound hexose and seromucoid levels were measured in 21 male Egyptian patients before and after treatment for schistosomiasis, and in 8 normal controls. Results indicate that alpha1 and gamma-glycoprotein were significantly higher while beta-glycoprotein was lower in bilharzial patients than in controls. Protein-bound hexose and seromucoid levels were also significantly elevated in the patients. After treatment, the levels of these fractions approached those of the controls.", "PMID": 961993}
{"id": "pubmed23n0032_4817", "title": "Chemotherapy of  hydatid disease (Echinococcus granulosus) in mice with mebendazole and bithionol.", "content": "We treated female Swiss Webster mice with heavy, long standing infections of Echinococcus granulosus with mebendazole, 50 mg/kg body weight daily for 10 days and bithionol, 70 mg/kg every other day for four doses. Necropsy performed 6 weeks after completion of therapy showed no gross or histologic differences between untreated controls and bithionol-treated mice or their cysts. Mebendazole-treated mice had a significant decrease in the total number of cysts (15 vs. 100), with many of those present being ruptured. Electron microscopy of intact, mebendazole-treated cysts revealed a marked increase in vacuolization and disarray of the distal cytoplasm, dilated and degenerating microtubules, increase in the size and number of lysosomes, a decrease in the number of normal appearing golgi, and increased density of mitochondria. Several mice died shortly after the termination of mebendazole therapy, all with ruptured cysts. Due to the large volume of the cysts, the presumed cause of death was acute volume overload, but toxic or anaphylactic reactions could not be excluded. We suggest that any proposed chemotherapy of humans with hydatid disease be done cautiously, with careful monitoring.", "contents": "Chemotherapy of  hydatid disease (Echinococcus granulosus) in mice with mebendazole and bithionol. We treated female Swiss Webster mice with heavy, long standing infections of Echinococcus granulosus with mebendazole, 50 mg/kg body weight daily for 10 days and bithionol, 70 mg/kg every other day for four doses. Necropsy performed 6 weeks after completion of therapy showed no gross or histologic differences between untreated controls and bithionol-treated mice or their cysts. Mebendazole-treated mice had a significant decrease in the total number of cysts (15 vs. 100), with many of those present being ruptured. Electron microscopy of intact, mebendazole-treated cysts revealed a marked increase in vacuolization and disarray of the distal cytoplasm, dilated and degenerating microtubules, increase in the size and number of lysosomes, a decrease in the number of normal appearing golgi, and increased density of mitochondria. Several mice died shortly after the termination of mebendazole therapy, all with ruptured cysts. Due to the large volume of the cysts, the presumed cause of death was acute volume overload, but toxic or anaphylactic reactions could not be excluded. We suggest that any proposed chemotherapy of humans with hydatid disease be done cautiously, with careful monitoring.", "PMID": 961994}
{"id": "pubmed23n0032_4818", "title": "Epidemic influenza in a hill tribe of northwest Thailand.", "content": "An investigation of an epidemic of respiratory disease in a remote region of northwest Thailand revealed Influenza A/H3N2 as the etiologic agent. This epidemic would not have been recognized were it not for an unusual increase in respiratory disease following a meeting attended by residents of many villages. The influenza strains isolated most closely resembled A/Port Chalmers/1/73 when tested by hemagglutination inhibition but showed consistent antigenic differences when tested by quantitative neutralization. It is suggested that the differences noted between this strain and influenza viruses isolated elsewhere may have been due to the sequential transmission of influenza through partially immune people.", "contents": "Epidemic influenza in a hill tribe of northwest Thailand. An investigation of an epidemic of respiratory disease in a remote region of northwest Thailand revealed Influenza A/H3N2 as the etiologic agent. This epidemic would not have been recognized were it not for an unusual increase in respiratory disease following a meeting attended by residents of many villages. The influenza strains isolated most closely resembled A/Port Chalmers/1/73 when tested by hemagglutination inhibition but showed consistent antigenic differences when tested by quantitative neutralization. It is suggested that the differences noted between this strain and influenza viruses isolated elsewhere may have been due to the sequential transmission of influenza through partially immune people.", "PMID": 961995}
{"id": "pubmed23n0032_4819", "title": "Endemic rabies in Ghana. Epidemiology and control measures.", "content": "Rabies is well established in Accra and there has been no decline in canine or human cases during the past 5 years. In the first 6 months of 1975, canine cases almost doubled over the period average. So far dogs are the only confirmed carnivorous vector in Ghana. A clear seasonal peak exists, so that intervention efforts can be times to achieve a maximum impact. Persons at greatest risk of contracting rabies are males and school-aged children, who need improved educational and post-dog bite services. The fundamental issue for health policy is to achieve a proper balance between environmental, preventive and treatment aspects of rabies. The report describes problems with logistics, canine vaccine shortage and failures, lack of owner cooperation, and control of a large stray dog population. Several proposals to improve coordination and organization of control programs are suggested.", "contents": "Endemic rabies in Ghana. Epidemiology and control measures. Rabies is well established in Accra and there has been no decline in canine or human cases during the past 5 years. In the first 6 months of 1975, canine cases almost doubled over the period average. So far dogs are the only confirmed carnivorous vector in Ghana. A clear seasonal peak exists, so that intervention efforts can be times to achieve a maximum impact. Persons at greatest risk of contracting rabies are males and school-aged children, who need improved educational and post-dog bite services. The fundamental issue for health policy is to achieve a proper balance between environmental, preventive and treatment aspects of rabies. The report describes problems with logistics, canine vaccine shortage and failures, lack of owner cooperation, and control of a large stray dog population. Several proposals to improve coordination and organization of control programs are suggested.", "PMID": 961996}
{"id": "pubmed23n0032_4820", "title": "Epidemiology of an outbreak of head lice in Georgia.", "content": "An outbreak of head lice infestation (pediculosis) occurred in elementary school children in Barrow County, Georgia, in January 1974. An investigation was initiated to define the magnitude of the outbreak, determine factors that contribute to transmission, and disseminate information on control. All elementary school children in the county were examined for head lice and answered a questionnaire. Fifty-three (3%) of 1,783 white pupils were infested, but none of the 500 black pupils was infested. Distribution of infestation in the white pupils was influenced by grade, bed-sharing, socioeconomic status, infestation of other family members, crowding in the home, and family size; distribution was not influenced by hair length or the sex of the pupil. Recommendations for control based on the results of the investigation included procedures for identifying and processing cases, distributing free pediculicides, continuing surveillance, educating school personnel and parents on how to control the parasite.", "contents": "Epidemiology of an outbreak of head lice in Georgia. An outbreak of head lice infestation (pediculosis) occurred in elementary school children in Barrow County, Georgia, in January 1974. An investigation was initiated to define the magnitude of the outbreak, determine factors that contribute to transmission, and disseminate information on control. All elementary school children in the county were examined for head lice and answered a questionnaire. Fifty-three (3%) of 1,783 white pupils were infested, but none of the 500 black pupils was infested. Distribution of infestation in the white pupils was influenced by grade, bed-sharing, socioeconomic status, infestation of other family members, crowding in the home, and family size; distribution was not influenced by hair length or the sex of the pupil. Recommendations for control based on the results of the investigation included procedures for identifying and processing cases, distributing free pediculicides, continuing surveillance, educating school personnel and parents on how to control the parasite.", "PMID": 961997}
{"id": "pubmed23n0032_4821", "title": "Species identification of blood meals from Culex tarsalis that had fed on passeriform birds.", "content": "Blood-engorged Culex tarsalis (4,209) collected during 1960 to 1965 in Kern County, California and previously shown to have fed on passeriform birds by the precipitin test were further tested by the more sensitive passive hemagglutination inhibition test (PHI). It was shown that these mosquitoes fed principally on House Finches and House Sparrows, the most common passeriform birds found in the collection areas. These data demonstrate the usefulness of the PHI test for the identification of arthropod blood meals from closely related species.", "contents": "Species identification of blood meals from Culex tarsalis that had fed on passeriform birds. Blood-engorged Culex tarsalis (4,209) collected during 1960 to 1965 in Kern County, California and previously shown to have fed on passeriform birds by the precipitin test were further tested by the more sensitive passive hemagglutination inhibition test (PHI). It was shown that these mosquitoes fed principally on House Finches and House Sparrows, the most common passeriform birds found in the collection areas. These data demonstrate the usefulness of the PHI test for the identification of arthropod blood meals from closely related species.", "PMID": 961998}
{"id": "pubmed23n0032_4822", "title": "Field evaluation of arthropod repellent netting as a ground cloth against ticks.", "content": "Tick repellent, wide-mesh net ground cloths were tested against Amblyomma americanum (Linnaeus) by the CO2 bait technique. Two repellents, N,N-diethyl-metatoluamide (deet) and 3 acetyl-2-(dimethyl-5-heptenyl)-oxazoladine, and two netting fabrics were compared. All combinations tested provided over 90% protection. The effectiveness of repellent ground cloths against chiggers is discussed.", "contents": "Field evaluation of arthropod repellent netting as a ground cloth against ticks. Tick repellent, wide-mesh net ground cloths were tested against Amblyomma americanum (Linnaeus) by the CO2 bait technique. Two repellents, N,N-diethyl-metatoluamide (deet) and 3 acetyl-2-(dimethyl-5-heptenyl)-oxazoladine, and two netting fabrics were compared. All combinations tested provided over 90% protection. The effectiveness of repellent ground cloths against chiggers is discussed.", "PMID": 961999}
{"id": "pubmed23n0032_4823", "title": "Comparison of morbidity interviews with a health examination survey in rural Africa.", "content": "This paper describes a study of the Danfa Comprehensive Rural Health and Family Planning Project, Ghana. The report compares information obtained from morbidity interviews with information obtained during subsequent health examinations. One to 4 days prior to examination by a team of physicians, 3,653 rural Ghanaians were interviewed by a team of auxiliary workers. Information obtained from the interview survey was noticeably different from examination diagnoses. Significant health problems such as malaria, intestinal parasites and diarrhea, as well as minor and chronic conditions were seriously under-reported. Interview findings were more accurate for children, women in the reproductive age group, and in cases in which the disease caused considerable discomfort or diability. Although individual examination was eight times as costly as an interview, small scale rural health examination surveys of a representative sample of the population are recommended to provide accurate morbidity information for health planners.", "contents": "Comparison of morbidity interviews with a health examination survey in rural Africa. This paper describes a study of the Danfa Comprehensive Rural Health and Family Planning Project, Ghana. The report compares information obtained from morbidity interviews with information obtained during subsequent health examinations. One to 4 days prior to examination by a team of physicians, 3,653 rural Ghanaians were interviewed by a team of auxiliary workers. Information obtained from the interview survey was noticeably different from examination diagnoses. Significant health problems such as malaria, intestinal parasites and diarrhea, as well as minor and chronic conditions were seriously under-reported. Interview findings were more accurate for children, women in the reproductive age group, and in cases in which the disease caused considerable discomfort or diability. Although individual examination was eight times as costly as an interview, small scale rural health examination surveys of a representative sample of the population are recommended to provide accurate morbidity information for health planners.", "PMID": 962000}
{"id": "pubmed23n0032_4824", "title": "Isolation of Leishmania from a black rat in the Baghdad area, Iraq.", "content": "Leishmania was recovered from a black rat in the Baghdad area, Iraq. Leptomonads from samples of blood and tissues of this animal cultivated on NNN medium produced visceral leishmaniasis when injected into white mice. This is the first record of natural visceral leishmaniasis in a non-human host in Iraq.", "contents": "Isolation of Leishmania from a black rat in the Baghdad area, Iraq. Leishmania was recovered from a black rat in the Baghdad area, Iraq. Leptomonads from samples of blood and tissues of this animal cultivated on NNN medium produced visceral leishmaniasis when injected into white mice. This is the first record of natural visceral leishmaniasis in a non-human host in Iraq.", "PMID": 962001}
{"id": "pubmed23n0032_4825", "title": "Oral transmission of Brugia pahangi to dogs.", "content": "Oral transmission of Brugia pahangi, already demonstrated in jirds, has now been accomplished in dogs. Beagle puppies, four anesthetized and two unanesthetized, were exposed to B. pahangi by instilling third-stage larvae (L-3s) into the mouth. Infections matured in all the dogs, and adult worms were recovered mainly from the mandibular, retropharyngeal, and axillary lymphatics. Worms were relatively numerous and peripheral microfilaremia developed in the dogs exposed under anesthetic, while worms were infrequent and microfilariae were found only intracardially in the dogs exposed without anesthetic. It appears that in orally exposed dogs, as in jirds studied earlier, the successful L-3s probably penetrated mucosa in or near the mouth.", "contents": "Oral transmission of Brugia pahangi to dogs. Oral transmission of Brugia pahangi, already demonstrated in jirds, has now been accomplished in dogs. Beagle puppies, four anesthetized and two unanesthetized, were exposed to B. pahangi by instilling third-stage larvae (L-3s) into the mouth. Infections matured in all the dogs, and adult worms were recovered mainly from the mandibular, retropharyngeal, and axillary lymphatics. Worms were relatively numerous and peripheral microfilaremia developed in the dogs exposed under anesthetic, while worms were infrequent and microfilariae were found only intracardially in the dogs exposed without anesthetic. It appears that in orally exposed dogs, as in jirds studied earlier, the successful L-3s probably penetrated mucosa in or near the mouth.", "PMID": 962002}
{"id": "pubmed23n0032_4826", "title": "Half-body radiotherapy of advanced cancer.", "content": "Half-body radiotherapy to deliver doses up to 1000 rads in a single exposure evolved to meet a need in treating patients with advanced cancer. Large fields are required and a high radiation output. Treatments are well tolerated, although radiation sickness occurs in 80% of upper half and 33% of lower half-body treatments. Marrow depression is not a problem and the second half body can be treated when the peripheral blood count has returned to normal. Subjective improvement occurs in most patients and this group had a median survival of six months.", "contents": "Half-body radiotherapy of advanced cancer. Half-body radiotherapy to deliver doses up to 1000 rads in a single exposure evolved to meet a need in treating patients with advanced cancer. Large fields are required and a high radiation output. Treatments are well tolerated, although radiation sickness occurs in 80% of upper half and 33% of lower half-body treatments. Marrow depression is not a problem and the second half body can be treated when the peripheral blood count has returned to normal. Subjective improvement occurs in most patients and this group had a median survival of six months.", "PMID": 962003}
{"id": "pubmed23n0032_4827", "title": "Localization of gallium-67 citrate in salivary glands following radiation therapy.", "content": "Gallium-67 citrate was found to localize in the salivary glands of eight patients after therapeutic doses of radiation to a treatment field including the glands. In two patients, autopsy evidence to the effect that no primary disease was present to explain such uptake was obtained. In another two patients the abnormal uptake was not observed on follow-up. The recognition that radiation sialadenitis is a potential cause of false-positive gallium scintiscans is important in their interpretation.", "contents": "Localization of gallium-67 citrate in salivary glands following radiation therapy. Gallium-67 citrate was found to localize in the salivary glands of eight patients after therapeutic doses of radiation to a treatment field including the glands. In two patients, autopsy evidence to the effect that no primary disease was present to explain such uptake was obtained. In another two patients the abnormal uptake was not observed on follow-up. The recognition that radiation sialadenitis is a potential cause of false-positive gallium scintiscans is important in their interpretation.", "PMID": 962004}
{"id": "pubmed23n0032_4828", "title": "Disease of the gallbladder in patients with normal cholecystograms.", "content": "In contrast with what has widely been assumed, disease of the gallbladder sometimes remains undertected by even repeated cholecystography or cholegraphy. Chronic cholecystitis, gallstones, and acalculous cholesterolosis can escape detection by roentgenography of the gallbladder. Of 4,000 patients subjected to cholecystectomy between 1962 and 1973, the cholecystogram had been normal in only two. The range of indications for the operation has since been widened. Patients with typical symptons of gallbladder disease or recurrent pancreatitis of unknown origin have been operated on despite a normal cholecystogram. Within as short a period as eight months, an additional six patients with a normal cholecstogram have been operated on at our hospitals. Treatment consisted of cholecystectomy also in the four patients in whom no stones could be palpated in the gallbladder. Gross and microscopic examination of the gallbladder in these patients revealed cholesterolosis. When last seen, seven of the eight patients were still symptom-free an average fifteen months after the operation.", "contents": "Disease of the gallbladder in patients with normal cholecystograms. In contrast with what has widely been assumed, disease of the gallbladder sometimes remains undertected by even repeated cholecystography or cholegraphy. Chronic cholecystitis, gallstones, and acalculous cholesterolosis can escape detection by roentgenography of the gallbladder. Of 4,000 patients subjected to cholecystectomy between 1962 and 1973, the cholecystogram had been normal in only two. The range of indications for the operation has since been widened. Patients with typical symptons of gallbladder disease or recurrent pancreatitis of unknown origin have been operated on despite a normal cholecystogram. Within as short a period as eight months, an additional six patients with a normal cholecstogram have been operated on at our hospitals. Treatment consisted of cholecystectomy also in the four patients in whom no stones could be palpated in the gallbladder. Gross and microscopic examination of the gallbladder in these patients revealed cholesterolosis. When last seen, seven of the eight patients were still symptom-free an average fifteen months after the operation.", "PMID": 962007}
{"id": "pubmed23n0032_4829", "title": "Diagnostic considerations in acute alcoholic and gallstone pancreatitis.", "content": "Comparison of a group of patients with acute alcoholic pancreatitis with a group with gallstone pancreatitis has established the serum amylase level on admission as one of the most useful laboratory tests in aiding to differentiate the two entities. A serum amylase level greater than 1,500 IU was most often due to gallstone pancreatitis, as was elevation of the serum bilirubin and alkaline phosphatase levels.", "contents": "Diagnostic considerations in acute alcoholic and gallstone pancreatitis. Comparison of a group of patients with acute alcoholic pancreatitis with a group with gallstone pancreatitis has established the serum amylase level on admission as one of the most useful laboratory tests in aiding to differentiate the two entities. A serum amylase level greater than 1,500 IU was most often due to gallstone pancreatitis, as was elevation of the serum bilirubin and alkaline phosphatase levels.", "PMID": 962008}
{"id": "pubmed23n0032_4830", "title": "Surgical management of impotence: progress report.", "content": "Our experience utilizing a new design Lash-Maser silicone implant in the surgical management of impotence has been detailed. The present series includes twenty-five patients, twenty-three of whom achieved satisfactory intercourse without problems or complications. The implant's simplicity of design, function, and surgical implantation is appealing.", "contents": "Surgical management of impotence: progress report. Our experience utilizing a new design Lash-Maser silicone implant in the surgical management of impotence has been detailed. The present series includes twenty-five patients, twenty-three of whom achieved satisfactory intercourse without problems or complications. The implant's simplicity of design, function, and surgical implantation is appealing.", "PMID": 962009}
{"id": "pubmed23n0032_4831", "title": "Surgical considerations in hyperparathyroidism: reappraisal of the need for multigland biopsy.", "content": "Sixty-seven cases of neck exploration for suspected hyperparathyroidism were reviewed. Thirty-nine patients underwent removal of an adenoma with biopsy of one or more other parathyroid glands. In another group, nine patients underwent removal of the adenoma only. Both groups have had no recurrences of hyperparathyroidism in follow-up periods of two months to twelve years. The data presented indicate that removal of a parathyroid adenoma alone, without biopsy of other tissue, represents satisfactory treatment. Experience with hyperplastic glands is also reviewed. Subtotal parathyroidectomy was effective treatment in all patients, but a 30 per cent incidence of hypocalcemia was noted after this operation.", "contents": "Surgical considerations in hyperparathyroidism: reappraisal of the need for multigland biopsy. Sixty-seven cases of neck exploration for suspected hyperparathyroidism were reviewed. Thirty-nine patients underwent removal of an adenoma with biopsy of one or more other parathyroid glands. In another group, nine patients underwent removal of the adenoma only. Both groups have had no recurrences of hyperparathyroidism in follow-up periods of two months to twelve years. The data presented indicate that removal of a parathyroid adenoma alone, without biopsy of other tissue, represents satisfactory treatment. Experience with hyperplastic glands is also reviewed. Subtotal parathyroidectomy was effective treatment in all patients, but a 30 per cent incidence of hypocalcemia was noted after this operation.", "PMID": 962010}
{"id": "pubmed23n0032_4832", "title": "Bone scans of the facial bones: normal anatomy.", "content": "Some features of facial anatomy have been identified on 99mTc polyphosphate bone scans by a comparison of scans, radiographs, and photographs. 99mTc polyphosphate bone scans are used clinically to show bone injury and increased osteoblastic activity; the primary use is to identify malignancy and other diseases involving bone. A knowledge of normal anatomy is of the utmost importance in the accurate interpretation of bone scans of the face.", "contents": "Bone scans of the facial bones: normal anatomy. Some features of facial anatomy have been identified on 99mTc polyphosphate bone scans by a comparison of scans, radiographs, and photographs. 99mTc polyphosphate bone scans are used clinically to show bone injury and increased osteoblastic activity; the primary use is to identify malignancy and other diseases involving bone. A knowledge of normal anatomy is of the utmost importance in the accurate interpretation of bone scans of the face.", "PMID": 962011}
{"id": "pubmed23n0032_4833", "title": "Pulmonary function studies after smoke inhalation.", "content": "Pulmonary function studies were performed within 72 hours of injury in seven patients with smoke inhalation injury diagnosed by positive 133Xe scintiphotographs and in eight patients with burns of similar size but with negative 133Xenon scans. The former patients showed decreased peak flow, decreased flow at 25, 50, and 75 per cent of vital capacity, and an elevated pulmonary resistance. In addition, single breath nitrogen tests revealed evidence of maldistribution of ventilation/perfusion abnormalities. Total lung capacity, functional residual capacity, and compliance (both dynamic and static) were similar in the two groups. Pulmonary function studies can be of assistance in evaluating smoke inhalation, estimating the severity, and following the course of patients with this disorder.", "contents": "Pulmonary function studies after smoke inhalation. Pulmonary function studies were performed within 72 hours of injury in seven patients with smoke inhalation injury diagnosed by positive 133Xe scintiphotographs and in eight patients with burns of similar size but with negative 133Xenon scans. The former patients showed decreased peak flow, decreased flow at 25, 50, and 75 per cent of vital capacity, and an elevated pulmonary resistance. In addition, single breath nitrogen tests revealed evidence of maldistribution of ventilation/perfusion abnormalities. Total lung capacity, functional residual capacity, and compliance (both dynamic and static) were similar in the two groups. Pulmonary function studies can be of assistance in evaluating smoke inhalation, estimating the severity, and following the course of patients with this disorder.", "PMID": 962012}
{"id": "pubmed23n0032_4834", "title": "The centrineurogenic etiology of the respiratory distress syndrome: induction by isolated cerebral hypoxemia and prevention by unilateral pulmonary denervation.", "content": "Twenty-eight anemic control dogs were subjected to isolated cerebral hypoxemic (PO2,35+/-5 mm Hg) perfusion for 2 hours. All were found to have functional pulmonary impairment. Two hours later, twenty were sacrificed and found to have the bilateral anatomic complex of the respiratory distress syndrome (RDS). All those not sacrificed expired within 20 hours with progressive respiratory distress and at autopsy had the bilateral anatomic complex. Twenty-three beagles with chronic denervation (autotransplantation) of the left lung also were subjected to the 2 hour isolated cerebral arterial hypoxemic perfusion. Minimal pulmonary functional impairment was measurable in all. Ten of sixteen were long-term survivors. The six that succumbed did not appear to suffer respiratory deaths. These six, as well as seven sacrificed 2 hours after perfusion, had the anatomic complex of RDS in the normally innervated right lungs. However, the denervated left lungs were anatomically normal. These findings are offered as additional evidence that RDS has a centrineurogenic etiology. We postulate the following sequence: \"shock\" causes cerebral (probably hypothalamic) cellular oxygen deprivation and dysfunction; there is autonomically mediated, increased resistance of the pulmonary venules (\"postcapillary sphincters\"); this leads to capillary hypertension, congestion, hemorrhage, edema, surfactant inactivation, and atelectasis. Pulmonary denervation blocks this sequence and protects the lung.", "contents": "The centrineurogenic etiology of the respiratory distress syndrome: induction by isolated cerebral hypoxemia and prevention by unilateral pulmonary denervation. Twenty-eight anemic control dogs were subjected to isolated cerebral hypoxemic (PO2,35+/-5 mm Hg) perfusion for 2 hours. All were found to have functional pulmonary impairment. Two hours later, twenty were sacrificed and found to have the bilateral anatomic complex of the respiratory distress syndrome (RDS). All those not sacrificed expired within 20 hours with progressive respiratory distress and at autopsy had the bilateral anatomic complex. Twenty-three beagles with chronic denervation (autotransplantation) of the left lung also were subjected to the 2 hour isolated cerebral arterial hypoxemic perfusion. Minimal pulmonary functional impairment was measurable in all. Ten of sixteen were long-term survivors. The six that succumbed did not appear to suffer respiratory deaths. These six, as well as seven sacrificed 2 hours after perfusion, had the anatomic complex of RDS in the normally innervated right lungs. However, the denervated left lungs were anatomically normal. These findings are offered as additional evidence that RDS has a centrineurogenic etiology. We postulate the following sequence: \"shock\" causes cerebral (probably hypothalamic) cellular oxygen deprivation and dysfunction; there is autonomically mediated, increased resistance of the pulmonary venules (\"postcapillary sphincters\"); this leads to capillary hypertension, congestion, hemorrhage, edema, surfactant inactivation, and atelectasis. Pulmonary denervation blocks this sequence and protects the lung.", "PMID": 962013}
{"id": "pubmed23n0032_4835", "title": "Nonanastomotic aneurysms in venous homologous grafts and bovine heterografts in femoropopliteal bypasses.", "content": "In the absence of a suitable autologous vein for a long distal femoropopliteal bypass, the homologous vein appears to be a good substitute. However, the patency rate after one year is less than 50 per cent and nonanastomotic aneurysms occurred in one third of our patients followed for more than one year. These two complications may be related to an immune response which may be lessened by preservation of the graft in the frozen state. Bovine heterografts function well when placed subfascially for femoropopliteal bypasses ending above the knee. Failure rate is high when the distal end of the graft extends across the knee joint. In our experience nonanastomotic aneurysms have developed in 50 per cent of patent grafts followed for more than one year, although other surgeons have reported an incidence of only 3 to 20 per cent. In the follow-up of twenty-three venous homografts and twenty bovine heterografts placed as femoropopliteal bypasses, eight nonanastomotic aneurysms appeared and required replacement. This occurrence was noted in more than 33 per cent of grafts patent beyond one year. When an adequate autologous vein is not available, the composite Dacron-vein graft is the best substitute.", "contents": "Nonanastomotic aneurysms in venous homologous grafts and bovine heterografts in femoropopliteal bypasses. In the absence of a suitable autologous vein for a long distal femoropopliteal bypass, the homologous vein appears to be a good substitute. However, the patency rate after one year is less than 50 per cent and nonanastomotic aneurysms occurred in one third of our patients followed for more than one year. These two complications may be related to an immune response which may be lessened by preservation of the graft in the frozen state. Bovine heterografts function well when placed subfascially for femoropopliteal bypasses ending above the knee. Failure rate is high when the distal end of the graft extends across the knee joint. In our experience nonanastomotic aneurysms have developed in 50 per cent of patent grafts followed for more than one year, although other surgeons have reported an incidence of only 3 to 20 per cent. In the follow-up of twenty-three venous homografts and twenty bovine heterografts placed as femoropopliteal bypasses, eight nonanastomotic aneurysms appeared and required replacement. This occurrence was noted in more than 33 per cent of grafts patent beyond one year. When an adequate autologous vein is not available, the composite Dacron-vein graft is the best substitute.", "PMID": 962014}
{"id": "pubmed23n0032_4836", "title": "Swelling of the hand after arteriovenous fistula for hemodialysis.", "content": "Despite a high patency rate and low incidence of infections, several complications have been described with the Brescia subcutaneous arteriovenous fistula. We recently observed five patients with swelling of the hand and wrist secondary to increased distal vein flow. Ligation of the distal vein, with or without proximal reconstruction resulted in cure. Proximal vein patency must be assessed.", "contents": "Swelling of the hand after arteriovenous fistula for hemodialysis. Despite a high patency rate and low incidence of infections, several complications have been described with the Brescia subcutaneous arteriovenous fistula. We recently observed five patients with swelling of the hand and wrist secondary to increased distal vein flow. Ligation of the distal vein, with or without proximal reconstruction resulted in cure. Proximal vein patency must be assessed.", "PMID": 962015}
{"id": "pubmed23n0032_4837", "title": "Idiopathic perforation of the colon in a newborn.", "content": "Although infrequent, idiopathic perforation of the colon in the newborn is a surgical emergency. Etiology remains obscure. Diagnosis of a pneumoperitoneum, prompt operative intervention, and the use of antibiotic therapy are essential to the survival of these infants.", "contents": "Idiopathic perforation of the colon in a newborn. Although infrequent, idiopathic perforation of the colon in the newborn is a surgical emergency. Etiology remains obscure. Diagnosis of a pneumoperitoneum, prompt operative intervention, and the use of antibiotic therapy are essential to the survival of these infants.", "PMID": 962017}
{"id": "pubmed23n0032_4838", "title": "Spontaneous return of function after surgical excision of the seventh cranial nerve in parotid tumor surgery.", "content": "Spontaneous return of function after surgical excision of the seventh cranial nerve in surgery of parotid tumors is not impossible. This seems to occur through the several existing neural pathways between the fifth and seventh cranial nerves. It is probable that voluntary motor impulses by reeducation find their way from the cortex through the fifth cranial nerve to the respective muscles.", "contents": "Spontaneous return of function after surgical excision of the seventh cranial nerve in parotid tumor surgery. Spontaneous return of function after surgical excision of the seventh cranial nerve in surgery of parotid tumors is not impossible. This seems to occur through the several existing neural pathways between the fifth and seventh cranial nerves. It is probable that voluntary motor impulses by reeducation find their way from the cortex through the fifth cranial nerve to the respective muscles.", "PMID": 962018}
{"id": "pubmed23n0032_4839", "title": "The renal compression syndrome.", "content": "A clinical example of perirenal compression producing documented renin-mediated hypertension is presented. The pathophysiology of this disorder is discussed as it relates to renin release mechanisms. In the future, recognition of the renal compression syndrome may obviate needless nephrectomies since simple removal of the source of compression should result in the cure of hypertension.", "contents": "The renal compression syndrome. A clinical example of perirenal compression producing documented renin-mediated hypertension is presented. The pathophysiology of this disorder is discussed as it relates to renin release mechanisms. In the future, recognition of the renal compression syndrome may obviate needless nephrectomies since simple removal of the source of compression should result in the cure of hypertension.", "PMID": 962019}
{"id": "pubmed23n0032_4840", "title": "Pancreatic trauma: a new diagnostic approach.", "content": "The difficulties encountered in the diagnosis of solitary pancreatic injury when there is no other indication for surgical exploration of the abdomen are discussed. We suggest that endoscopic transduodenal pancreatography is a reliable diagnostic tool of great help in evaluating such injuries with little morbidity.", "contents": "Pancreatic trauma: a new diagnostic approach. The difficulties encountered in the diagnosis of solitary pancreatic injury when there is no other indication for surgical exploration of the abdomen are discussed. We suggest that endoscopic transduodenal pancreatography is a reliable diagnostic tool of great help in evaluating such injuries with little morbidity.", "PMID": 962020}
{"id": "pubmed23n0032_4841", "title": "Unusual presentation of hydatid disease.", "content": "Hydatid disease is endemic in most areas in Iran where sheep are raised, and diagnosis is fairly easy in a majority of cases. However, this fairly common disease may become very difficult and even impossible to diagnose. The most important factor in diagnosing hydatid disease is the awareness of its possibility. The striking clinical resemblance between hydatid disease and malignant diseases of some organs makes the correct diagnosis essential. In countries where this disease is endemic, any growing mass or tumor should arouse suspicion of hydatid disease.", "contents": "Unusual presentation of hydatid disease. Hydatid disease is endemic in most areas in Iran where sheep are raised, and diagnosis is fairly easy in a majority of cases. However, this fairly common disease may become very difficult and even impossible to diagnose. The most important factor in diagnosing hydatid disease is the awareness of its possibility. The striking clinical resemblance between hydatid disease and malignant diseases of some organs makes the correct diagnosis essential. In countries where this disease is endemic, any growing mass or tumor should arouse suspicion of hydatid disease.", "PMID": 962021}
{"id": "pubmed23n0032_4842", "title": "Acute venous hemodialysis using the unipuncture apparatus.", "content": "Hemodialysis is a well recognized treatment modality for the support of patients with acute renal failure. In such patients, rapid access to the circulation for hemodialysis is important. For those patients with self-limited acute renal failure in whom recovery is expected, a rapid means of gaining temporary access to the circulation would be particularly desirable. The Seldinger technic for femoral vein catheterization and the use of the unipuncture dialysis apparatus have been combined to meet this requirement. The efficiency of unipuncture dialysis through a single femoral catheter compares favorably with the efficiency of dialysis by the standard two catheter technic.", "contents": "Acute venous hemodialysis using the unipuncture apparatus. Hemodialysis is a well recognized treatment modality for the support of patients with acute renal failure. In such patients, rapid access to the circulation for hemodialysis is important. For those patients with self-limited acute renal failure in whom recovery is expected, a rapid means of gaining temporary access to the circulation would be particularly desirable. The Seldinger technic for femoral vein catheterization and the use of the unipuncture dialysis apparatus have been combined to meet this requirement. The efficiency of unipuncture dialysis through a single femoral catheter compares favorably with the efficiency of dialysis by the standard two catheter technic.", "PMID": 962022}
{"id": "pubmed23n0032_4843", "title": "Functional longevity of intraperioneal drains: an experimental evaluation.", "content": "Various types of drains were inserted into the peritoneal cavity of twenty-eight dogs. After one to seven days, all drains failed to show the presence of 200 cc of colored fluid injected intraperitoneally. On autopsy, all tubes were surrounded and occluded by omentum.", "contents": "Functional longevity of intraperioneal drains: an experimental evaluation. Various types of drains were inserted into the peritoneal cavity of twenty-eight dogs. After one to seven days, all drains failed to show the presence of 200 cc of colored fluid injected intraperitoneally. On autopsy, all tubes were surrounded and occluded by omentum.", "PMID": 962023}
{"id": "pubmed23n0032_4844", "title": "[On the mechanism of action of acupuncture analgesia (author's transl)].", "content": "The correlation between the effects of acupuncture (A) and hypnosuggestion (H) on subjective pain perception was studied in normal volunteers using somatosensory evoked potentials technique. Both methods influenced identically the subjective pain perception and affected the evoked cortical responses similarly. In those subjects in whom analgesia was achieved, location of the A needles proved unimportant and the suppression of the cortical response elicited by A or H was identical. This could be explained by cortically induced inhibitory activity in descending control systems for both methods. A. analgesia thus appears to be a suggestive modality which depends on individual susceptibility. A specific cultural background also seems to be of importance.", "contents": "[On the mechanism of action of acupuncture analgesia (author's transl)]. The correlation between the effects of acupuncture (A) and hypnosuggestion (H) on subjective pain perception was studied in normal volunteers using somatosensory evoked potentials technique. Both methods influenced identically the subjective pain perception and affected the evoked cortical responses similarly. In those subjects in whom analgesia was achieved, location of the A needles proved unimportant and the suppression of the cortical response elicited by A or H was identical. This could be explained by cortically induced inhibitory activity in descending control systems for both methods. A. analgesia thus appears to be a suggestive modality which depends on individual susceptibility. A specific cultural background also seems to be of importance.", "PMID": 962033}
{"id": "pubmed23n0032_4845", "title": "[Clinical and experimental results with acupuncture-analgesia (author's transl)].", "content": "Operations under acupuncture analgesia have been performed in Vienna since March 1972. A total of 102 procedures has been performed with \"classical\" Chinese acupuncture analgesia. Additionally given analgesics, psychodynamic factors and neurophysiological components are of joint importance. Our success rate in these cases was 64 p.c. Since October 1972 we call \"two-phase-acupuncture-analgesia\". Up to June 1975 72 operations were performed under some modifications of this method. In a neurophysiological study 17 volunteers and in 2 patients undergoing stereothalamotomies depression or even disappearance of evoked potentials was be observed during acupuncture with alectrical stimulation. Thus an interaction between pain stimuli and acupuncture stimuli in the thalamus could for the first time be demonstrated in man.", "contents": "[Clinical and experimental results with acupuncture-analgesia (author's transl)]. Operations under acupuncture analgesia have been performed in Vienna since March 1972. A total of 102 procedures has been performed with \"classical\" Chinese acupuncture analgesia. Additionally given analgesics, psychodynamic factors and neurophysiological components are of joint importance. Our success rate in these cases was 64 p.c. Since October 1972 we call \"two-phase-acupuncture-analgesia\". Up to June 1975 72 operations were performed under some modifications of this method. In a neurophysiological study 17 volunteers and in 2 patients undergoing stereothalamotomies depression or even disappearance of evoked potentials was be observed during acupuncture with alectrical stimulation. Thus an interaction between pain stimuli and acupuncture stimuli in the thalamus could for the first time be demonstrated in man.", "PMID": 962034}
{"id": "pubmed23n0032_4846", "title": "[Combined acupuncture-analgesia as a method in general anesthesia (author's transl)].", "content": "The clinical results of 162 operations performed with combined-acupuncture-analgesia are reported. The experiences so collected are satisfying. This method seems suitable for anaesthetic practice. Total absence of toxity good analgesia and the negligible circulatory reactions give this method advantages in the anesthetic treatment of old and poor risk patients. A heavy disadvantage is that the method in time consuming is expensive in personnel.", "contents": "[Combined acupuncture-analgesia as a method in general anesthesia (author's transl)]. The clinical results of 162 operations performed with combined-acupuncture-analgesia are reported. The experiences so collected are satisfying. This method seems suitable for anaesthetic practice. Total absence of toxity good analgesia and the negligible circulatory reactions give this method advantages in the anesthetic treatment of old and poor risk patients. A heavy disadvantage is that the method in time consuming is expensive in personnel.", "PMID": 962035}
{"id": "pubmed23n0032_4847", "title": "[Changes of bloodchemical parameters of healthy volunteers under influence of acupuncture (author's transl)].", "content": "The influence of acupuncture on some biochemical parameters in blood was investigated in healthy volunteers. These results were compared with two control series. The significant increase especially of free fatty acids concentration must be considered as an effect of acupuncture.", "contents": "[Changes of bloodchemical parameters of healthy volunteers under influence of acupuncture (author's transl)]. The influence of acupuncture on some biochemical parameters in blood was investigated in healthy volunteers. These results were compared with two control series. The significant increase especially of free fatty acids concentration must be considered as an effect of acupuncture.", "PMID": 962036}
{"id": "pubmed23n0032_4848", "title": "[On the application of electric stimulation currents in operations. Concept of a clinically applicable device (author's transl)].", "content": "With the use of high voltage electric currents in patients who initally have been anaesthetised, the anaesthesiologist faces new aspects. For this reason basic principles of physical medicine and medical engineering with respect to special problems are described. The majority of impulses current generators used so far do not even comply with the first principal requirement of any therapy namely knowledge of the dose of the agent applied. A system for electric currents crossing the body is outlined, which is characterized by constant current and isovoltage-free outlets in connection with a special ECG-amplifier and a control pattern. This apparatus for the first time guarantees maximal safety in clinical use. First animal experiments demonstrate the possibility of the risk if this method is applied in a inexperienced manner: in all animals ventricular fibrillation could be achieved when the electrodes were used in a position near the heart and there appeared to occur nonspecific reactions when current was directed through the brain (ear acupuncture)", "contents": "[On the application of electric stimulation currents in operations. Concept of a clinically applicable device (author's transl)]. With the use of high voltage electric currents in patients who initally have been anaesthetised, the anaesthesiologist faces new aspects. For this reason basic principles of physical medicine and medical engineering with respect to special problems are described. The majority of impulses current generators used so far do not even comply with the first principal requirement of any therapy namely knowledge of the dose of the agent applied. A system for electric currents crossing the body is outlined, which is characterized by constant current and isovoltage-free outlets in connection with a special ECG-amplifier and a control pattern. This apparatus for the first time guarantees maximal safety in clinical use. First animal experiments demonstrate the possibility of the risk if this method is applied in a inexperienced manner: in all animals ventricular fibrillation could be achieved when the electrodes were used in a position near the heart and there appeared to occur nonspecific reactions when current was directed through the brain (ear acupuncture)", "PMID": 962037}
{"id": "pubmed23n0032_4849", "title": "[Observations on the course of leucocytes in cardiac surgery under acupuncture-analgesia (author's transl)].", "content": "In 11 cardiosurgical patients leucocyte and differential counts were made performed during and after surgery. In some cases Lymphocyte transformation and migration inhibitiontest was studied. Comparing cases under conventional neurolept anaesthesia with those who had acupuncture Analgesia (electrostimulation) it appeared that the number of leucocytes was rising significantly higher in the latter, although the total number of lymphocytes remained constant. In lymphocyte transformation and mirgration inhibitiontest the observed decrease seemed to be less pronounced. Because a trial applying acupuncture in healthy persons resulted in no change in the number of leucocytes, it is suggested that the surgical trauma and related catecholamine release might be of importance. No definite result could be obtained.", "contents": "[Observations on the course of leucocytes in cardiac surgery under acupuncture-analgesia (author's transl)]. In 11 cardiosurgical patients leucocyte and differential counts were made performed during and after surgery. In some cases Lymphocyte transformation and migration inhibitiontest was studied. Comparing cases under conventional neurolept anaesthesia with those who had acupuncture Analgesia (electrostimulation) it appeared that the number of leucocytes was rising significantly higher in the latter, although the total number of lymphocytes remained constant. In lymphocyte transformation and mirgration inhibitiontest the observed decrease seemed to be less pronounced. Because a trial applying acupuncture in healthy persons resulted in no change in the number of leucocytes, it is suggested that the surgical trauma and related catecholamine release might be of importance. No definite result could be obtained.", "PMID": 962038}
{"id": "pubmed23n0032_4850", "title": "[Electrical stimulation anaesthesia in abdominal surgery in special consideration of selective proximal vagotomie (author's transl)].", "content": "107 abdominal operations were performed using in a combined electrostimulating anaesthesia. Anaesthesia was indued by etomidate (about 15 mg) and fentanly (about 0,2 mg). After administration of pancuronium (5-7 mg) intubation was performed. During continuous intermittent relaxation anaesthesia was maintained by a mixture of nitrous oxide/oxygen 4:2 (in poor risk patients: 3:3) and electrical stimulation at certain body-and ear-points. Electrical stimulation was done using impulse currents with frequencies of 10 to 15 Hz and a current intensity of about 40 mA. During extremly painful periods of operations 30% of the patients showed maximal heart rates and only in 5% of cases did blood pressure exceed preoperative values by about 50%. Especially in poor risk pateints the results of electro-stimulating anaesthesia are excellent. At this time, however, it should not be recommanded as a routine method for general surgery; questions concerning regulating mechanisms need to be answered first.", "contents": "[Electrical stimulation anaesthesia in abdominal surgery in special consideration of selective proximal vagotomie (author's transl)]. 107 abdominal operations were performed using in a combined electrostimulating anaesthesia. Anaesthesia was indued by etomidate (about 15 mg) and fentanly (about 0,2 mg). After administration of pancuronium (5-7 mg) intubation was performed. During continuous intermittent relaxation anaesthesia was maintained by a mixture of nitrous oxide/oxygen 4:2 (in poor risk patients: 3:3) and electrical stimulation at certain body-and ear-points. Electrical stimulation was done using impulse currents with frequencies of 10 to 15 Hz and a current intensity of about 40 mA. During extremly painful periods of operations 30% of the patients showed maximal heart rates and only in 5% of cases did blood pressure exceed preoperative values by about 50%. Especially in poor risk pateints the results of electro-stimulating anaesthesia are excellent. At this time, however, it should not be recommanded as a routine method for general surgery; questions concerning regulating mechanisms need to be answered first.", "PMID": 962039}
{"id": "pubmed23n0032_4851", "title": "Adrenal cortical hormones in the spermatic vein of 95 patients with left varicocele.", "content": "Adrenocortical hormones were measured in the peripheral and spermatic venous blood samples, taken simultaneously at the time of a high vasoligation for left varicocele in 95 patients. Our results do not confirm the hypothesis of MacLeod that the deleterious effects of a varicocele on male fertility are caused by an higher local concentration of adrenocortical hormones, due to venous reflux through incompetent valves. If this is the case it occurs only in a minority of patients.", "contents": "Adrenal cortical hormones in the spermatic vein of 95 patients with left varicocele. Adrenocortical hormones were measured in the peripheral and spermatic venous blood samples, taken simultaneously at the time of a high vasoligation for left varicocele in 95 patients. Our results do not confirm the hypothesis of MacLeod that the deleterious effects of a varicocele on male fertility are caused by an higher local concentration of adrenocortical hormones, due to venous reflux through incompetent valves. If this is the case it occurs only in a minority of patients.", "PMID": 962168}
{"id": "pubmed23n0032_4852", "title": "Kinetics of the male gametogenesis.", "content": "The kinetics of the male gametogenesis during the pregonadal period, prespermatogenesis, and \"early\" spermatogenesis has been described in detail. Concerning spermatogenesis in the adult individual reference is made to the articles of Courot, Hochereau-de Reviers and Ortavant (1970) and of Clermont (1972). The comparison of female and male gametogenesis (Fig. 1) shows that the \"gonia stage\" (asterisks) of the female germ cells is limited to one proliferation wave only, whereas the \"gonia stage\" of the male germ cells consists of a first proliferation wave, comparable to that of oogonia, a preparative phase to initiate spermatogenesis, and a second proliferation wave with renewal and differentiation of the spermatogonia. Germ cells in the \"gonia stage\" are highly sensitive towards ionising radiation and cytostatic drugs.", "contents": "Kinetics of the male gametogenesis. The kinetics of the male gametogenesis during the pregonadal period, prespermatogenesis, and \"early\" spermatogenesis has been described in detail. Concerning spermatogenesis in the adult individual reference is made to the articles of Courot, Hochereau-de Reviers and Ortavant (1970) and of Clermont (1972). The comparison of female and male gametogenesis (Fig. 1) shows that the \"gonia stage\" (asterisks) of the female germ cells is limited to one proliferation wave only, whereas the \"gonia stage\" of the male germ cells consists of a first proliferation wave, comparable to that of oogonia, a preparative phase to initiate spermatogenesis, and a second proliferation wave with renewal and differentiation of the spermatogonia. Germ cells in the \"gonia stage\" are highly sensitive towards ionising radiation and cytostatic drugs.", "PMID": 962169}
{"id": "pubmed23n0032_4853", "title": "Comparative cytomorphologic aspects of the male germ cells, especially of the \"Gonia\".", "content": "During early development of the human testis the male germ cell first appears as primordial germ cell or gonocyte and enters the prospermatogonial stage after the twelfth week of gestation. Prospermatogonia lack glycogen particles and are connected by intercellular bridges. During the same period in the ovary the female germ cell undergoes its oogonial stage. Prospermatogonial and oogonial development have been studied histologically in three laboratory species (white mouse, Acomys caharhinus dimidiatus. golden hamster). According to the therminolgy of Hilscher et al. (1974) proposed for the rat, prospermatogonia have to be subdivided into M- T (1)- and T (2)-prospermatogonia. In the ovary they correspond to oogonia, oocytes in the prophase of meiosis and oocytes in the diplotene stage.", "contents": "Comparative cytomorphologic aspects of the male germ cells, especially of the \"Gonia\". During early development of the human testis the male germ cell first appears as primordial germ cell or gonocyte and enters the prospermatogonial stage after the twelfth week of gestation. Prospermatogonia lack glycogen particles and are connected by intercellular bridges. During the same period in the ovary the female germ cell undergoes its oogonial stage. Prospermatogonial and oogonial development have been studied histologically in three laboratory species (white mouse, Acomys caharhinus dimidiatus. golden hamster). According to the therminolgy of Hilscher et al. (1974) proposed for the rat, prospermatogonia have to be subdivided into M- T (1)- and T (2)-prospermatogonia. In the ovary they correspond to oogonia, oocytes in the prophase of meiosis and oocytes in the diplotene stage.", "PMID": 962170}
{"id": "pubmed23n0032_4854", "title": "Longitudinal growth of the seminiferous tubules in LH and FSH treated rats.", "content": "New born Sprague Dawley rats were injected daily either with 50 (n=8), 100 (9) or 200 (10) mug FSH; with 50 (9), 100 (5), 200 (10) mug LH; with a combination of 50 mug FSH + 50 mug LH (10); or with the vehicle 0.9% saline (10). The animals were sacrificied at an age of 16 days. There was no influence of the hormones on the body weight. FSH caused a dose dependant stimulation of the testes growth (163, 186, 227 mg for the 50, 100 and 200 mug group; control 139 mg; diff. to control P less than or equal to 0.05, 0.01, and 0.01), whereas LH had no effect (123, 154 and 133 mg for the 50, 100 and 200 mug. LH group). The weight of the seminal vesicles was increased slightly by LH only (Diff. to control P less than 0l01). The serum androgen level was not changed by any of the treatments. The stage of the spermatogenesis was not altered by the hormones. There was no significant difference in the diameter of the seminiferous tubules. The higher testes weight after FSH administration was caused by an increase of length of the seminiferous tubules (25 m, 30 m and 35 m for 50 mug, 100 mug, and 200 mug FSH; control 23 m; diff.: P less than or equal to 0.05, less than or equal to 0.01, less than or equal to 0.01). The conclusion is that FSH has a specific effect on the longitudinal growth of the seminiferous tubules in prepubertal rats.", "contents": "Longitudinal growth of the seminiferous tubules in LH and FSH treated rats. New born Sprague Dawley rats were injected daily either with 50 (n=8), 100 (9) or 200 (10) mug FSH; with 50 (9), 100 (5), 200 (10) mug LH; with a combination of 50 mug FSH + 50 mug LH (10); or with the vehicle 0.9% saline (10). The animals were sacrificied at an age of 16 days. There was no influence of the hormones on the body weight. FSH caused a dose dependant stimulation of the testes growth (163, 186, 227 mg for the 50, 100 and 200 mug group; control 139 mg; diff. to control P less than or equal to 0.05, 0.01, and 0.01), whereas LH had no effect (123, 154 and 133 mg for the 50, 100 and 200 mug. LH group). The weight of the seminal vesicles was increased slightly by LH only (Diff. to control P less than 0l01). The serum androgen level was not changed by any of the treatments. The stage of the spermatogenesis was not altered by the hormones. There was no significant difference in the diameter of the seminiferous tubules. The higher testes weight after FSH administration was caused by an increase of length of the seminiferous tubules (25 m, 30 m and 35 m for 50 mug, 100 mug, and 200 mug FSH; control 23 m; diff.: P less than or equal to 0.05, less than or equal to 0.01, less than or equal to 0.01). The conclusion is that FSH has a specific effect on the longitudinal growth of the seminiferous tubules in prepubertal rats.", "PMID": 962171}
{"id": "pubmed23n0032_4855", "title": "Variation in the stock of testicular stem cells and in the yield of spermatogonial divisions in ram and bull testes.", "content": "Two types of stem spermatogonia- type A0 and A1 spermatogonia- have been observed in ram and bull testes. Type A0 spermatogonia are present in the impuberal testis. Their total number/testis does not vary greatly during life of bull. Type A0 spermatogonia give rise to type A1 spermatogonia which appear at the onset of spermatogenesis; their total number/testis increases during and after puberty. Total numbers of type A0 and A1 spermatogonia/testis are not correlated in the ram while total numbers of type A1 spermatogonia and Sertoli cells are correlated. Hemicastration performed in impuberal calves induces an increase in numbers of Sertoli cell/type A1 spermatogonia/testis. In adult ram, the total number of A0 and A1 spermatogonia decreases during the non sexual season and increase again in the sexual season. After hypophysectomy performed in adult rams, the number of A0 spermatogonia/testis is not modified while that of A1 spermatogonia decreases. In ram and bull, 6 spermatogonial generations were detected before the germ cells undergo meiosis. In the adult ram and bull, the type A1 spermatogonia originate mostly after divisions of A2 spermatogonia. Few of them arise from A0 spermatogonia. The yield of primary spermatocytes from spermatogonial divisions is very low, one third and one half of the expected number in the bull and ram respectively. In ram, thus yield positively correlated to LH plasma levels (0.5; P = 0.05). In hypophysectomized ram, this yield is nearly equal to zero.", "contents": "Variation in the stock of testicular stem cells and in the yield of spermatogonial divisions in ram and bull testes. Two types of stem spermatogonia- type A0 and A1 spermatogonia- have been observed in ram and bull testes. Type A0 spermatogonia are present in the impuberal testis. Their total number/testis does not vary greatly during life of bull. Type A0 spermatogonia give rise to type A1 spermatogonia which appear at the onset of spermatogenesis; their total number/testis increases during and after puberty. Total numbers of type A0 and A1 spermatogonia/testis are not correlated in the ram while total numbers of type A1 spermatogonia and Sertoli cells are correlated. Hemicastration performed in impuberal calves induces an increase in numbers of Sertoli cell/type A1 spermatogonia/testis. In adult ram, the total number of A0 and A1 spermatogonia decreases during the non sexual season and increase again in the sexual season. After hypophysectomy performed in adult rams, the number of A0 spermatogonia/testis is not modified while that of A1 spermatogonia decreases. In ram and bull, 6 spermatogonial generations were detected before the germ cells undergo meiosis. In the adult ram and bull, the type A1 spermatogonia originate mostly after divisions of A2 spermatogonia. Few of them arise from A0 spermatogonia. The yield of primary spermatocytes from spermatogonial divisions is very low, one third and one half of the expected number in the bull and ram respectively. In ram, thus yield positively correlated to LH plasma levels (0.5; P = 0.05). In hypophysectomized ram, this yield is nearly equal to zero.", "PMID": 962172}
{"id": "pubmed23n0032_4856", "title": "Kinetics of histone and protamine synthesis during meiosis and spermiogenesis in the mouse.", "content": "The separation of mouse spermatogenic cell nuclei by sedimentation velocity at unit gravity has been used to determine the timing of histone and \"mouse protamine\" synthesis, and the turnover of basic nuclear proteins throughout spermatogenesis. Animals were injected with 3H-arginine or 3H-lysine and at various time intervals (2 hours post-label or from 1 to 30 days post-label) germinal cell nuclei preparations were separated on the staput. Labelled histones and mouse protamine were extracted from staput separated nuclei with hydrocholoric acid and fractionated by polyacrylamide gel electrophoresis. Results indicate that histones are synthesized in association with DNA replication in spermatogonia and preleptotene spermatocytes, in pachytene primary spermatocytes and in spermatids stages 11-16, simultaneously with \"mouse protamine\". Experiments are reported showing that histones synthesized in pachytene primary spermatocytes and in spermatids stages 11-16 are retained in epididymal spermatozoa, while histones synthesized before meiosis are no longer detectable onto chromatin after meiosis.", "contents": "Kinetics of histone and protamine synthesis during meiosis and spermiogenesis in the mouse. The separation of mouse spermatogenic cell nuclei by sedimentation velocity at unit gravity has been used to determine the timing of histone and \"mouse protamine\" synthesis, and the turnover of basic nuclear proteins throughout spermatogenesis. Animals were injected with 3H-arginine or 3H-lysine and at various time intervals (2 hours post-label or from 1 to 30 days post-label) germinal cell nuclei preparations were separated on the staput. Labelled histones and mouse protamine were extracted from staput separated nuclei with hydrocholoric acid and fractionated by polyacrylamide gel electrophoresis. Results indicate that histones are synthesized in association with DNA replication in spermatogonia and preleptotene spermatocytes, in pachytene primary spermatocytes and in spermatids stages 11-16, simultaneously with \"mouse protamine\". Experiments are reported showing that histones synthesized in pachytene primary spermatocytes and in spermatids stages 11-16 are retained in epididymal spermatozoa, while histones synthesized before meiosis are no longer detectable onto chromatin after meiosis.", "PMID": 962173}
{"id": "pubmed23n0032_4857", "title": "Ultrastructural observations on the differentiation of spermatids in man.", "content": "Ultrastructural aspects of the normal development of human spermatids are presented. Eight typical pictures of differentiation of spermatids are described based on cytological details of the acrosome, nucleus and tail. The transient appearance of a spindle-shaped body, connected to the principal piece of the tail, is pointed out. In most cases, malformed spermatids have developmental disturbances of only one component of the cell, e.g. of the acrosome, nucleus or tail. Some typical malformations are described.", "contents": "Ultrastructural observations on the differentiation of spermatids in man. Ultrastructural aspects of the normal development of human spermatids are presented. Eight typical pictures of differentiation of spermatids are described based on cytological details of the acrosome, nucleus and tail. The transient appearance of a spindle-shaped body, connected to the principal piece of the tail, is pointed out. In most cases, malformed spermatids have developmental disturbances of only one component of the cell, e.g. of the acrosome, nucleus or tail. Some typical malformations are described.", "PMID": 962174}
{"id": "pubmed23n0032_4858", "title": "Soluble proteins of guinea pig seminal vesicle lumen: purification and partial characterization.", "content": "Luminal contents of guinea pig seminal vesicle were extracted with 0.154 M NaCl and the soluble protein fraction was studied by DEAE-cellulose and Sephadex G-100 column chromatography; anionic, cationic, and SDS-polyarcylamide disc gel electrophoresis; isoelectric focusing; molecular weight determinations by SDS-polyacrylamide disc gel electrophoresis; analysis for neutral carbohydrate; sucrose density-gradient centrifugation for determination of sedimentation coefficients; and amino acid analyses. Four protein fractions (referred to as proteins 1, 2, 3, and 4 in order to elution) were obtained by DEAE-cellulose column chromatography. Proteins 1, 2, and 4 gave single major bands in multiple disc gel electrophoretic systems. Protein 3 gave one major band and one minor component believed to be a fragment of a primary vesicular protein. Protein 1 is very basic and probably represents the clotting protein of guinea pig semen. The three nonclotting proteins could not be detected in serum and are probably intrinsic to seminal vesicle epithelium. All four proteins were found in animals weighing 700 g or more; only two of the three nonclotting proteins were found in more than half of the younger animals. This makes it even more likely that one or more of the nonclotting proteinc could serve as a useful gene marker in studies of androgen mechanisms.", "contents": "Soluble proteins of guinea pig seminal vesicle lumen: purification and partial characterization. Luminal contents of guinea pig seminal vesicle were extracted with 0.154 M NaCl and the soluble protein fraction was studied by DEAE-cellulose and Sephadex G-100 column chromatography; anionic, cationic, and SDS-polyarcylamide disc gel electrophoresis; isoelectric focusing; molecular weight determinations by SDS-polyacrylamide disc gel electrophoresis; analysis for neutral carbohydrate; sucrose density-gradient centrifugation for determination of sedimentation coefficients; and amino acid analyses. Four protein fractions (referred to as proteins 1, 2, 3, and 4 in order to elution) were obtained by DEAE-cellulose column chromatography. Proteins 1, 2, and 4 gave single major bands in multiple disc gel electrophoretic systems. Protein 3 gave one major band and one minor component believed to be a fragment of a primary vesicular protein. Protein 1 is very basic and probably represents the clotting protein of guinea pig semen. The three nonclotting proteins could not be detected in serum and are probably intrinsic to seminal vesicle epithelium. All four proteins were found in animals weighing 700 g or more; only two of the three nonclotting proteins were found in more than half of the younger animals. This makes it even more likely that one or more of the nonclotting proteinc could serve as a useful gene marker in studies of androgen mechanisms.", "PMID": 962175}
{"id": "pubmed23n0032_4859", "title": "[Animal experimental studies on the effects of noise stress on male fertility].", "content": "Psychic stress is able to cause male infertility. Stieve found out aspermatogenesis in executed men. Cockett et al. immobilized 5 Macaca-Monkeys 15 to 40 days in capsule module under simulated space flight conditions and detected severe testicular degeneration. Carosi and Calabro reported about workers exposed to industrial noise over a period of years; the number of offspring was found to be much lower than in non exposed families. We examined the effect of noise on the fertility of 21 male guinea pigs. Sounding of 110 dB lasted 3-5 hours daily over a period of 22-31 days. Histological examination of the testicular tissue did not show any disorder of spermatogenesis. We believe that the negative result is attributed to failings in the test arrangement. Sounding only lasted for a maximum of 31 days. However, the duration of spermatogenesis in guinea pigs is about 40 days. Moreover the sounding maximum was only 5 hours per day, yet the time of recovery took 4-5 times longer every day. Consequently the daily short-term depression of the neuroendrocrinium affected the gonadotropine secretion in similarity to the daily biorhythmic variation. Therefore the expected inhibition of fertility by the neuroendrocrinic way could not be demonstrated. Our experiments also revealed another problem. In noise-exposed guinea pigs there was 8.2 per cent tubuli contorti without complete spermatogenesis. Moreover, in the control group even 9.9 per cent of the tubuli was found to be sperm-free. It is not clear why such a contrary reaction occurred. However, it is possible that hormonal stimulation increased temporarily as a result of noise stress. It should be noted that this effect has also been reported by Arguellis.", "contents": "[Animal experimental studies on the effects of noise stress on male fertility]. Psychic stress is able to cause male infertility. Stieve found out aspermatogenesis in executed men. Cockett et al. immobilized 5 Macaca-Monkeys 15 to 40 days in capsule module under simulated space flight conditions and detected severe testicular degeneration. Carosi and Calabro reported about workers exposed to industrial noise over a period of years; the number of offspring was found to be much lower than in non exposed families. We examined the effect of noise on the fertility of 21 male guinea pigs. Sounding of 110 dB lasted 3-5 hours daily over a period of 22-31 days. Histological examination of the testicular tissue did not show any disorder of spermatogenesis. We believe that the negative result is attributed to failings in the test arrangement. Sounding only lasted for a maximum of 31 days. However, the duration of spermatogenesis in guinea pigs is about 40 days. Moreover the sounding maximum was only 5 hours per day, yet the time of recovery took 4-5 times longer every day. Consequently the daily short-term depression of the neuroendrocrinium affected the gonadotropine secretion in similarity to the daily biorhythmic variation. Therefore the expected inhibition of fertility by the neuroendrocrinic way could not be demonstrated. Our experiments also revealed another problem. In noise-exposed guinea pigs there was 8.2 per cent tubuli contorti without complete spermatogenesis. Moreover, in the control group even 9.9 per cent of the tubuli was found to be sperm-free. It is not clear why such a contrary reaction occurred. However, it is possible that hormonal stimulation increased temporarily as a result of noise stress. It should be noted that this effect has also been reported by Arguellis.", "PMID": 962176}
{"id": "pubmed23n0032_4860", "title": "Halothane-induced decrease in experimental myocardial ischemia in the non-failing canine heart.", "content": "The effect of halothane on net myocardial oxygen balance of ischemic myocardium was studied in the non-failing canine heart. Myocardial ischemia was produced by repeated reversible occlusions of a coronary artery; the severity of ischemia was estimated by summating ST-segment elevations (sigma ST) obtained by epicardial ECG mapping at 15 to 18 sites. Control measurements were obtained before and after administration of halothane (0.75 per cent) to six dogs with chloralose-urethane basal anesthesia. Halothane was associated with significant decreases of systemic arterial pressure (P less than .001), heart rate (P less than .01), and the product of systolic arterial pressure X heart rate (P less than .01), an indirect index of myocardial oxygen consumption, while left atrial pressure remained unchanged at normal levels. sigmaST during occlusion was less (P less .001) during halothane (26.5 +/- 7.4 (SD) mv) than before (36.6 +/- 5.4 mv) or after (34.4 +/- 8.2 mv) its administration. Thus, halothane decreased the severity of experimentally-induced myocardial ischemia in the non-failing canine heart. The data suggest that, in the absence of ventricular failure, halothane influences the relationship between myocardial oxygen supply and demand in a favorable direction when coronary blood flow is limited.", "contents": "Halothane-induced decrease in experimental myocardial ischemia in the non-failing canine heart. The effect of halothane on net myocardial oxygen balance of ischemic myocardium was studied in the non-failing canine heart. Myocardial ischemia was produced by repeated reversible occlusions of a coronary artery; the severity of ischemia was estimated by summating ST-segment elevations (sigma ST) obtained by epicardial ECG mapping at 15 to 18 sites. Control measurements were obtained before and after administration of halothane (0.75 per cent) to six dogs with chloralose-urethane basal anesthesia. Halothane was associated with significant decreases of systemic arterial pressure (P less than .001), heart rate (P less than .01), and the product of systolic arterial pressure X heart rate (P less than .01), an indirect index of myocardial oxygen consumption, while left atrial pressure remained unchanged at normal levels. sigmaST during occlusion was less (P less .001) during halothane (26.5 +/- 7.4 (SD) mv) than before (36.6 +/- 5.4 mv) or after (34.4 +/- 8.2 mv) its administration. Thus, halothane decreased the severity of experimentally-induced myocardial ischemia in the non-failing canine heart. The data suggest that, in the absence of ventricular failure, halothane influences the relationship between myocardial oxygen supply and demand in a favorable direction when coronary blood flow is limited.", "PMID": 962178}
{"id": "pubmed23n0032_4861", "title": "Onset of succinylcholine-induced hyperkalemia following denervation.", "content": "Denervation injuries in baboons were used to define the time course of the hyperkalemic response to succinylcholine. Half-peak increase in serum potassium (2.78 mEq/l) occurred 8.4 days following injury. Peak increase (5.5 mEq/l) appeared 14 days after injury. However, changes in potassium levels begin as early as four days after injury. Succinylcholine or other depolarizing muscle relaxants should not be used after the fourth day following an injury or denervation that involves two or more limbs.", "contents": "Onset of succinylcholine-induced hyperkalemia following denervation. Denervation injuries in baboons were used to define the time course of the hyperkalemic response to succinylcholine. Half-peak increase in serum potassium (2.78 mEq/l) occurred 8.4 days following injury. Peak increase (5.5 mEq/l) appeared 14 days after injury. However, changes in potassium levels begin as early as four days after injury. Succinylcholine or other depolarizing muscle relaxants should not be used after the fourth day following an injury or denervation that involves two or more limbs.", "PMID": 962179}
{"id": "pubmed23n0032_4862", "title": "The effects of diazepam on cerebral blood flow and oxygen consumption in rats and its synergistic interaction with nitrous oxide.", "content": "The effects of diazepam on cerebral blood flow (CBF) and cerebral oxygen uptake (CMRO2) was studied using a 133xenon modification of the Kety-Schmidt (1948) technique in paralyzed, artifically ventilated rats with and without simultaneous administration of 70 per cent nitrous oxide. Diazepam was given iv in doses that induced light to heavy sedation or general anesthesia. When given with 70 per cent nitrous oxide, diazepam in sedative and anesthetic doses lowered CBF and CMRO2 to about 60 per cent of control. In the absence of nitrous oxide all doses of diazepam caused moderate (20-30 per cent) decreases in CBF, but CMRO2 remained unchanged or was only slightly lowered. It is concluded that diazepam interacts with nitrous oxide to produce a reduction in CMRO2 similar to that seen in barbiturate anesthesia, but that alone the drug produces sedation and anesthesia without a comparable decrease in CMRO2.", "contents": "The effects of diazepam on cerebral blood flow and oxygen consumption in rats and its synergistic interaction with nitrous oxide. The effects of diazepam on cerebral blood flow (CBF) and cerebral oxygen uptake (CMRO2) was studied using a 133xenon modification of the Kety-Schmidt (1948) technique in paralyzed, artifically ventilated rats with and without simultaneous administration of 70 per cent nitrous oxide. Diazepam was given iv in doses that induced light to heavy sedation or general anesthesia. When given with 70 per cent nitrous oxide, diazepam in sedative and anesthetic doses lowered CBF and CMRO2 to about 60 per cent of control. In the absence of nitrous oxide all doses of diazepam caused moderate (20-30 per cent) decreases in CBF, but CMRO2 remained unchanged or was only slightly lowered. It is concluded that diazepam interacts with nitrous oxide to produce a reduction in CMRO2 similar to that seen in barbiturate anesthesia, but that alone the drug produces sedation and anesthesia without a comparable decrease in CMRO2.", "PMID": 962180}
{"id": "pubmed23n0032_4863", "title": "Sodium nitroprusside: pharmacology, toxicology and therapeutics.", "content": "Sodium nitroprusside is a potent, effective, and readily reversible direct vasodilating agent. It is broken down by hemoglobin into cyanide, which is in part detoxified by liver and kidney to thiocyanate. Some cyanide, especially in nitroprusside- \"resistant\" individuals who need large amounts of the drug, appears to remain free to cause cyanide poisoning. Patients requiring inordinate amounts probably should not continue to receive the drug, although maximum dosage limits for long-term therapy are not established. Blood thiocyanate levels do not indicate the extent to which free cyanide is limiting oxygen utilization in essential tissue, nor do blood cyanide levels. Metabolic acidosis, elevated lactate levels, elevated lactate/pyruvate ratios, and elevated mixed venous blood oxygen content are at present the best indications of the presence of cyanide poisoning during nitroprusside administration. Nitroprusside appears useful for induction of hypotension during surgery, and for treatment of hypertensive emergencies from all causes, although continuance for more than a few days is probably unwise. The reductions of cardiac afterload and ventricular filling pressure by nitroprusside appear useful in treatment of severe myocardial failure or infarction, but studies of myocardial cyanide toxicity are needed before complete acceptance of this therapy is warranted. Initial dose rates between 0.5 and 1.5 mug/kg/min are recommended only as starting points for very careful titration. Total projected intra-operative dosage should be calculated as quickly as possible and should not exceed 3-3.5 mg/kg. It is hoped that future studies will reveal the maximum dose of nitroprusside that can safely be metabolized in a 24-hour period, and may indicate that cofactors of rhodanase such as thiosulfate, or cobalamins such as hydroxocobalamin, can be administered with nitroprusside to prevent cyanide poisoning.", "contents": "Sodium nitroprusside: pharmacology, toxicology and therapeutics. Sodium nitroprusside is a potent, effective, and readily reversible direct vasodilating agent. It is broken down by hemoglobin into cyanide, which is in part detoxified by liver and kidney to thiocyanate. Some cyanide, especially in nitroprusside- \"resistant\" individuals who need large amounts of the drug, appears to remain free to cause cyanide poisoning. Patients requiring inordinate amounts probably should not continue to receive the drug, although maximum dosage limits for long-term therapy are not established. Blood thiocyanate levels do not indicate the extent to which free cyanide is limiting oxygen utilization in essential tissue, nor do blood cyanide levels. Metabolic acidosis, elevated lactate levels, elevated lactate/pyruvate ratios, and elevated mixed venous blood oxygen content are at present the best indications of the presence of cyanide poisoning during nitroprusside administration. Nitroprusside appears useful for induction of hypotension during surgery, and for treatment of hypertensive emergencies from all causes, although continuance for more than a few days is probably unwise. The reductions of cardiac afterload and ventricular filling pressure by nitroprusside appear useful in treatment of severe myocardial failure or infarction, but studies of myocardial cyanide toxicity are needed before complete acceptance of this therapy is warranted. Initial dose rates between 0.5 and 1.5 mug/kg/min are recommended only as starting points for very careful titration. Total projected intra-operative dosage should be calculated as quickly as possible and should not exceed 3-3.5 mg/kg. It is hoped that future studies will reveal the maximum dose of nitroprusside that can safely be metabolized in a 24-hour period, and may indicate that cofactors of rhodanase such as thiosulfate, or cobalamins such as hydroxocobalamin, can be administered with nitroprusside to prevent cyanide poisoning.", "PMID": 962181}
{"id": "pubmed23n0032_4864", "title": "A flask for preparation of standards in gas chromatography.", "content": "An inexpensive modification to the stopcock of a conventional gas-sampling bulb permits convenient preparation of standards for gas chromatography.", "contents": "A flask for preparation of standards in gas chromatography. An inexpensive modification to the stopcock of a conventional gas-sampling bulb permits convenient preparation of standards for gas chromatography.", "PMID": 962182}
{"id": "pubmed23n0032_4865", "title": "Genotype and retention of the ejaculatory reflex in castrated male mice.", "content": "Genotype proves to be an important factor affecting the retention of sexual responsiveness following castration. Male F1 hybrids between C57BL/6 females and DBA/2 males (B6D2F1) show very prolonged retention. Experiment 1 used a diallelic design using C57BL/6, DBA/2 and BALB/c strains and showed that heterosis per se is not an adequate explanation of the superior retention of the B6D2F1 genotype. Experiment 2 showed that neonatal injections of 100 mug TP to B6D2F1 male mice did not result in better retention of the ejaculatory reflex after castration in adulthood. B6D2F1 males from both experiments who exhibited the ejaculatory reflex for many months after castration went through a 'difficult period' in the first 10 to 12 postcastration weeks. During this time, ejaculation latency increased fourfold, and then returned to the original level. On the other hand, the proportion of males reaching the ejaculatory threshold during this period first declined then increased.", "contents": "Genotype and retention of the ejaculatory reflex in castrated male mice. Genotype proves to be an important factor affecting the retention of sexual responsiveness following castration. Male F1 hybrids between C57BL/6 females and DBA/2 males (B6D2F1) show very prolonged retention. Experiment 1 used a diallelic design using C57BL/6, DBA/2 and BALB/c strains and showed that heterosis per se is not an adequate explanation of the superior retention of the B6D2F1 genotype. Experiment 2 showed that neonatal injections of 100 mug TP to B6D2F1 male mice did not result in better retention of the ejaculatory reflex after castration in adulthood. B6D2F1 males from both experiments who exhibited the ejaculatory reflex for many months after castration went through a 'difficult period' in the first 10 to 12 postcastration weeks. During this time, ejaculation latency increased fourfold, and then returned to the original level. On the other hand, the proportion of males reaching the ejaculatory threshold during this period first declined then increased.", "PMID": 962190}
{"id": "pubmed23n0032_4866", "title": "Effects of adrenalectomy on the sexual behaviour of castrated and intact BDF1 mice.", "content": "Two experiments investigate the effects of adrenalectomy on the capacity of male mice of the BDF1 genotype (C57B1/6Fa male X DBA/2 female) to retain sexual behaviour following castration. Adrenalectomy is without effect and it is suggested that androgens can play no part in the maintenance of sexual behaviour in these castrates.", "contents": "Effects of adrenalectomy on the sexual behaviour of castrated and intact BDF1 mice. Two experiments investigate the effects of adrenalectomy on the capacity of male mice of the BDF1 genotype (C57B1/6Fa male X DBA/2 female) to retain sexual behaviour following castration. Adrenalectomy is without effect and it is suggested that androgens can play no part in the maintenance of sexual behaviour in these castrates.", "PMID": 962191}
{"id": "pubmed23n0032_4867", "title": "Postcastration retention of sexual behaviour in the male BDF1 mouse: the role of experience.", "content": "Male BDF1 mice (the F1 progeny of a cross between C57BL/6 females with DBA/2 males) show a remarkable retention of sexual behaviour following castration. Two experiments were conducted to describe in detail the postcastration copulatory performance of the BDF1 male mouse and to determine to what extent such performance is influenced by experience prior to castration. Experiment I found that castration leads to significant increases  in the number of mounts and intromissions needed to reach ejaculation, and to a significant increase in ejaculation latency. Experiment II found that although precastrational sexual experience is not essential for the performance of the ejaculatory reflex after castration, it does influence the frequency of its occurrence. Furthermore, type of post-weaning social experience influenced the display of ejaculatory behaviour by non-experienced castrates, as those with female social experience were superior to those with social experience with males or no social experience. The interactions of experience, hormones and genotype in the control of sexual behaviour in the BDF1 male are discussed.", "contents": "Postcastration retention of sexual behaviour in the male BDF1 mouse: the role of experience. Male BDF1 mice (the F1 progeny of a cross between C57BL/6 females with DBA/2 males) show a remarkable retention of sexual behaviour following castration. Two experiments were conducted to describe in detail the postcastration copulatory performance of the BDF1 male mouse and to determine to what extent such performance is influenced by experience prior to castration. Experiment I found that castration leads to significant increases  in the number of mounts and intromissions needed to reach ejaculation, and to a significant increase in ejaculation latency. Experiment II found that although precastrational sexual experience is not essential for the performance of the ejaculatory reflex after castration, it does influence the frequency of its occurrence. Furthermore, type of post-weaning social experience influenced the display of ejaculatory behaviour by non-experienced castrates, as those with female social experience were superior to those with social experience with males or no social experience. The interactions of experience, hormones and genotype in the control of sexual behaviour in the BDF1 male are discussed.", "PMID": 962192}
{"id": "pubmed23n0032_4868", "title": "Communication and spatial relationships in a colony of common grackles.", "content": "Most communication among common grackles Quiscalus quiscula occurs at distances of less than a few metres in the noisy environment of a breeding colony. This report examines both the adaptations of communication to these conditions and the effects of communication in regulating individual's spatial relationships. For each of six vocalizations and five action patterns studied in one colony, I consider variation in the form of the display, the circumstances associated with its use, and the responses it elicits. Each individual, male or female, has one characteristic, stereotyped song pattern that would facilitate individual recognition between mates. Variation in the components of vocalizations and action patterns is of two kinds: unidimensional, with either covarying or nested components, or multidimensional, with independently varying components, alternatives that have different implications for communication. The wide-spectrum sounds made by common grackles offer advantages in close-range communication in colonies, because the ease of locating such signals would minimize the cocktail-party effect, although they would have disadvantages in long-range communication. Most vocalizations of common grackles lack assocations with specific responses or external contexts, a situation that should often characterize short-range communication between acquainted individuals. The responses to vocalizations vary with context, especially the initial spatial relationships and identities of the interactors.", "contents": "Communication and spatial relationships in a colony of common grackles. Most communication among common grackles Quiscalus quiscula occurs at distances of less than a few metres in the noisy environment of a breeding colony. This report examines both the adaptations of communication to these conditions and the effects of communication in regulating individual's spatial relationships. For each of six vocalizations and five action patterns studied in one colony, I consider variation in the form of the display, the circumstances associated with its use, and the responses it elicits. Each individual, male or female, has one characteristic, stereotyped song pattern that would facilitate individual recognition between mates. Variation in the components of vocalizations and action patterns is of two kinds: unidimensional, with either covarying or nested components, or multidimensional, with independently varying components, alternatives that have different implications for communication. The wide-spectrum sounds made by common grackles offer advantages in close-range communication in colonies, because the ease of locating such signals would minimize the cocktail-party effect, although they would have disadvantages in long-range communication. Most vocalizations of common grackles lack assocations with specific responses or external contexts, a situation that should often characterize short-range communication between acquainted individuals. The responses to vocalizations vary with context, especially the initial spatial relationships and identities of the interactors.", "PMID": 962193}
{"id": "pubmed23n0032_4869", "title": "The effects of pregnancy on asthma: a prospective study.", "content": "Forty-seven pregnant asthmatics were studied in a prospective study. Maternal asthma was exacerbated in 43%, most often in the last trimester. Normal physiologic alterations of pregnancy are reviewed as plausable explanations for the course of asthmatic patients during pregnancy.", "contents": "The effects of pregnancy on asthma: a prospective study. Forty-seven pregnant asthmatics were studied in a prospective study. Maternal asthma was exacerbated in 43%, most often in the last trimester. Normal physiologic alterations of pregnancy are reviewed as plausable explanations for the course of asthmatic patients during pregnancy.", "PMID": 962194}
{"id": "pubmed23n0032_4870", "title": "The impact of chronic asthma on the developing child: observations made in a group setting.", "content": "Fourteen asthmatic children between 11 and 14 years of age participated in weekly group discussion sessions for nine months. The purposes were to allow open discussion about asthma, to mutually educate the children and staff about the medical and social implications of their disease and to give the patients a chance to deal with adults without fear of reprimand or betrayal of trust. Anger, envy, fear, sadness and guilt were the emotions predominant in the sessions. Group sessions may offer a further modality in the total care of the asthmatic child.", "contents": "The impact of chronic asthma on the developing child: observations made in a group setting. Fourteen asthmatic children between 11 and 14 years of age participated in weekly group discussion sessions for nine months. The purposes were to allow open discussion about asthma, to mutually educate the children and staff about the medical and social implications of their disease and to give the patients a chance to deal with adults without fear of reprimand or betrayal of trust. Anger, envy, fear, sadness and guilt were the emotions predominant in the sessions. Group sessions may offer a further modality in the total care of the asthmatic child.", "PMID": 962195}
{"id": "pubmed23n0032_4871", "title": "Prolonged bleeding times after aspirin ingestion in patients with aspirin idiosyncrasy.", "content": "Measurements of the bleeding time after the ingestion of small doses of aspirin in patients with aspirin idiosyncrasy demonstrate a marked prolongation compared with control patients. Confirmation of this finding offers a clue to the pathogenesis of this condition. Despite the prolonged bleeding time, gastrointestinal haemorrhage is an uncommon event in patients with aspirin idiosyncrasy.", "contents": "Prolonged bleeding times after aspirin ingestion in patients with aspirin idiosyncrasy. Measurements of the bleeding time after the ingestion of small doses of aspirin in patients with aspirin idiosyncrasy demonstrate a marked prolongation compared with control patients. Confirmation of this finding offers a clue to the pathogenesis of this condition. Despite the prolonged bleeding time, gastrointestinal haemorrhage is an uncommon event in patients with aspirin idiosyncrasy.", "PMID": 962198}
{"id": "pubmed23n0032_4872", "title": "Ultrastructural studies of prepubertal porcine uterine tube epithelium.", "content": "Ultrastructural details of prepubertal porcine uterine tube (oviduct) were studied in normal, growing gilts and compared with observations reported in other species. Tissues from the ampulla region of uterine tube were taken from 6 prepubertal gilts (106 to 139 days old) to determine cytodifferentiation of ciliated and secretory cells. The epithelium consisted of 2 distinctive cells, the ciliated and the secretory cells. Cilia were observed in the uterine tube of prepubertal gilts; however, degeneration of cilia was not observed in the present study. Most prominent observations were the occurrence of fibrous granules in the apical cytoplasm of ciliated cells. These fibrous granules contained electron-dense material and were present near basal bodies. The most unusual feature was the occurrence of procentrioles around a condensation form. These data indicate that ciliated cells are sensitive to estrogen. Intimate morphologic association between fibrous granules and basal bodies indicate that fibrous granules might provide precursor material for the development of cilia and rootlets. The cytoplasm of the secretory cells contained rough endoplasmic reticulum of tubular form and numerous ribosomes. Evidence for synthesis, storage, and release of secretory granules was not apparent. It is suggested that the secretory cells are not sensitive to the low, circulating concentration of plasma estrogen. The ultrastructure of the stromal cells and lymphatic capillary was described for the 1st time. The uterine tube stromal cells were characterized by prominent nucleus and a few cytoplasmic organelles. The lymphatic capillaries were distinguished by the blood capillaries, their much wider lumen, endothelium with an attenuated cytoplasm, absence of basal lamina, and overlapping and interdigitating intercellular junctions. The fine structure of the porcine uterine tube lymphatic capillary generally resembled that of other mammalian species.", "contents": "Ultrastructural studies of prepubertal porcine uterine tube epithelium. Ultrastructural details of prepubertal porcine uterine tube (oviduct) were studied in normal, growing gilts and compared with observations reported in other species. Tissues from the ampulla region of uterine tube were taken from 6 prepubertal gilts (106 to 139 days old) to determine cytodifferentiation of ciliated and secretory cells. The epithelium consisted of 2 distinctive cells, the ciliated and the secretory cells. Cilia were observed in the uterine tube of prepubertal gilts; however, degeneration of cilia was not observed in the present study. Most prominent observations were the occurrence of fibrous granules in the apical cytoplasm of ciliated cells. These fibrous granules contained electron-dense material and were present near basal bodies. The most unusual feature was the occurrence of procentrioles around a condensation form. These data indicate that ciliated cells are sensitive to estrogen. Intimate morphologic association between fibrous granules and basal bodies indicate that fibrous granules might provide precursor material for the development of cilia and rootlets. The cytoplasm of the secretory cells contained rough endoplasmic reticulum of tubular form and numerous ribosomes. Evidence for synthesis, storage, and release of secretory granules was not apparent. It is suggested that the secretory cells are not sensitive to the low, circulating concentration of plasma estrogen. The ultrastructure of the stromal cells and lymphatic capillary was described for the 1st time. The uterine tube stromal cells were characterized by prominent nucleus and a few cytoplasmic organelles. The lymphatic capillaries were distinguished by the blood capillaries, their much wider lumen, endothelium with an attenuated cytoplasm, absence of basal lamina, and overlapping and interdigitating intercellular junctions. The fine structure of the porcine uterine tube lymphatic capillary generally resembled that of other mammalian species.", "PMID": 962202}
{"id": "pubmed23n0032_4873", "title": "Sequential changes of estrogens and progesterone at prostaglandin-induced parturition in the sow.", "content": "Parenteral administration of prostaglandin F2alpha (PGF2alpha) is efficient in inducing parturition in late pregnant swine. The purpose in the present study was to investigate the sequential changes of progesterone and estrogens at prostaglandin-induced parturition in the sow. Six of 7 sows (86%) treated once intramuscularly with 12.5 mg of PGF2alpha on days 110, 111, 112, or 113 of pregnancy farrowed within 39 hours after the injection. One PGF2alpha-treated sow and 2 sows given placebo injections on days 110 and 111 of pregnancy farrowed 90 to 130 hours later. The injection of PGF2alpha decreased the peripheral plasma concentration of progesterone in a manner similar to that observed immediately prepartum in normally farrowing sows. Estrogen patterns were similar in PGF2alpha-treated and control sows.", "contents": "Sequential changes of estrogens and progesterone at prostaglandin-induced parturition in the sow. Parenteral administration of prostaglandin F2alpha (PGF2alpha) is efficient in inducing parturition in late pregnant swine. The purpose in the present study was to investigate the sequential changes of progesterone and estrogens at prostaglandin-induced parturition in the sow. Six of 7 sows (86%) treated once intramuscularly with 12.5 mg of PGF2alpha on days 110, 111, 112, or 113 of pregnancy farrowed within 39 hours after the injection. One PGF2alpha-treated sow and 2 sows given placebo injections on days 110 and 111 of pregnancy farrowed 90 to 130 hours later. The injection of PGF2alpha decreased the peripheral plasma concentration of progesterone in a manner similar to that observed immediately prepartum in normally farrowing sows. Estrogen patterns were similar in PGF2alpha-treated and control sows.", "PMID": 962203}
{"id": "pubmed23n0032_4874", "title": "Decoquinate in the control of experimentally induced coccidiosis of calves.", "content": "Decoquinate administered orally in a grain mix at dosages of 0.5, 0.538, 0.7, and 0.8 mg/kg of body weight suppressed oocyst discharge and bloody diarrhea in calves inoculated 3 days later with 100,000 oocysts of Eimeria bovis (experiment 1, n = 12 calves) or with 100,000 oocysts each of E bovis and Eimeria zuernii (experiment 2, n = 16 calves). Doses of 0.1, 0.163, 0.243, 0.3, and 0.362 mg/kg of body weight gave only partial suppression of oocyst discharge and diarrhea. Clinical signs of coccidiosis did not recur for 23 days after the treatment was discontinued.", "contents": "Decoquinate in the control of experimentally induced coccidiosis of calves. Decoquinate administered orally in a grain mix at dosages of 0.5, 0.538, 0.7, and 0.8 mg/kg of body weight suppressed oocyst discharge and bloody diarrhea in calves inoculated 3 days later with 100,000 oocysts of Eimeria bovis (experiment 1, n = 12 calves) or with 100,000 oocysts each of E bovis and Eimeria zuernii (experiment 2, n = 16 calves). Doses of 0.1, 0.163, 0.243, 0.3, and 0.362 mg/kg of body weight gave only partial suppression of oocyst discharge and diarrhea. Clinical signs of coccidiosis did not recur for 23 days after the treatment was discontinued.", "PMID": 962204}
{"id": "pubmed23n0032_4875", "title": "Changes of biochemical constituents in bovine fetal fluids with gestational age.", "content": "Serum, amniotic fluid, allantoic fluid, stomach contents, and urine were taken from 141 bovine fetuses 120 to 245 days old. The concentrations of the biochemical constituents were examined in tests to ascertain systematic changes with increasing crown-rump length, i.e., gestational age. As the crown-rump length increased, glucose concentrations decreased in allantoic fluid, amniotic fluid, and stomach contents; creatinine and uric acid concentrations increased in serum, allantoic fluid, and stomach contents; thyroxine concentrations increased in serum and allantoic fluid; potassium decreased in amniotic fluid and stomach contents; and total iron-binding capacity increased in amniotic fluid. The data in the present report can be used to identify the fetal fluid obtained by amniocentesis, to estimate the age of the fetus, and to aid the diagnosis of nutritional or infective processes that might affect the fetus.", "contents": "Changes of biochemical constituents in bovine fetal fluids with gestational age. Serum, amniotic fluid, allantoic fluid, stomach contents, and urine were taken from 141 bovine fetuses 120 to 245 days old. The concentrations of the biochemical constituents were examined in tests to ascertain systematic changes with increasing crown-rump length, i.e., gestational age. As the crown-rump length increased, glucose concentrations decreased in allantoic fluid, amniotic fluid, and stomach contents; creatinine and uric acid concentrations increased in serum, allantoic fluid, and stomach contents; thyroxine concentrations increased in serum and allantoic fluid; potassium decreased in amniotic fluid and stomach contents; and total iron-binding capacity increased in amniotic fluid. The data in the present report can be used to identify the fetal fluid obtained by amniocentesis, to estimate the age of the fetus, and to aid the diagnosis of nutritional or infective processes that might affect the fetus.", "PMID": 962205}
{"id": "pubmed23n0032_4876", "title": "Cutaneous hypersensitivity and isoantibody production in cattle injected with live or inactivated Anaplasma marginale in bovine and ovine erythrocytes.", "content": "Delayed cutaneous hypersensitivity and the production of isoagglutinins against normal bovine erythrocytes were measured in cattle inoculated with erythrocytic preparations containing live or inactivated Anaplasma marginale. The effect of blood group substances versus parasitic components on induction of delayed cutaneous hypersensitivity and isoimmunity was examined by using test antigens derived from normal and parasitized erythrocytes, virulent A marginale in bovine erythrocytes, and attenuated A marginale in ovine erythrocytes. The use of inactivated vaccine of bovine origin induced production of isoantibodies and cutaneous hypersensitivity to both bovine and ovine blood group factors, whereas a similar vaccine preparation of ovine origin stimulated antibodies and cutaneous reactivity only against ovine blood group factors.", "contents": "Cutaneous hypersensitivity and isoantibody production in cattle injected with live or inactivated Anaplasma marginale in bovine and ovine erythrocytes. Delayed cutaneous hypersensitivity and the production of isoagglutinins against normal bovine erythrocytes were measured in cattle inoculated with erythrocytic preparations containing live or inactivated Anaplasma marginale. The effect of blood group substances versus parasitic components on induction of delayed cutaneous hypersensitivity and isoimmunity was examined by using test antigens derived from normal and parasitized erythrocytes, virulent A marginale in bovine erythrocytes, and attenuated A marginale in ovine erythrocytes. The use of inactivated vaccine of bovine origin induced production of isoantibodies and cutaneous hypersensitivity to both bovine and ovine blood group factors, whereas a similar vaccine preparation of ovine origin stimulated antibodies and cutaneous reactivity only against ovine blood group factors.", "PMID": 962206}
{"id": "pubmed23n0032_4877", "title": "Utilization of proteins by the equine species.", "content": "Protein digestion, nitrogen retention, plasma protein, plasma urea, and plasma-free amino acids were determined for ponies fed 3 different protein supplements. Substitution of casein, corn gluten meal, or corn gluten meal plus lysine for a portion of the cornstarch in a low-protein basal ration increased apparent digestion of protein. Substitution with either casein or corn gluten meal plus lysine produced a significant increase (P less than 0.05) in nitrogen retention, whereas the corn gluten meal substitution did not. Nitrogen retention, expressed as percentage of nitrogen absorbed, was increased significantly (P less than 0.05) by the addition of casein to the basal ration. Significant change in plasma protein concentration did not occur when different sources of protein were added to the basal ration, but plasma urea nitrogen increased significantly (P less than 0.05) in the ponies given the casein variation of the ration. Lysine supplementation resulted in a marked increase in plasma lysine concentration. Seemingly the equine species responds to differences in the quality of dietary protein.", "contents": "Utilization of proteins by the equine species. Protein digestion, nitrogen retention, plasma protein, plasma urea, and plasma-free amino acids were determined for ponies fed 3 different protein supplements. Substitution of casein, corn gluten meal, or corn gluten meal plus lysine for a portion of the cornstarch in a low-protein basal ration increased apparent digestion of protein. Substitution with either casein or corn gluten meal plus lysine produced a significant increase (P less than 0.05) in nitrogen retention, whereas the corn gluten meal substitution did not. Nitrogen retention, expressed as percentage of nitrogen absorbed, was increased significantly (P less than 0.05) by the addition of casein to the basal ration. Significant change in plasma protein concentration did not occur when different sources of protein were added to the basal ration, but plasma urea nitrogen increased significantly (P less than 0.05) in the ponies given the casein variation of the ration. Lysine supplementation resulted in a marked increase in plasma lysine concentration. Seemingly the equine species responds to differences in the quality of dietary protein.", "PMID": 962207}
{"id": "pubmed23n0032_4878", "title": "Feline glomeruli: morphologic comparisons in normal, autolytic, and diseased kidneys.", "content": "Twelve cats were used to study autolytic changes in glomerular morphology and compare these with lesions of naturally occurring feline renal disease. The 12 cats had normal clinical, urinary, and blood features. One kidney (0-hour control) was excised immediately after a given cat was euthanatized, and portions of it were prepared for light and electron microscopy. The opposite kidney (autolytic) remained in situ for selected postmortem intervals, up to 24 hours, at which time it was similarly processed. Renal tissues from 4 additional cats (3 with proteinuria and 1 with diabetes mellitus) were processed and examined for comparison. Zero-hour control kidneys had the following mean quantitations: renal weight was 9.9 g; glomerular diameter, 83 mum; number of cells per glomerulus in 1-mum section was 63; and diameter of cell nuclei was 6.3 mum for mesangial, 6.7 mum for visceral epithelial, and 6.4 mum for endothelial. In comparison with 0-hour control kidneys, autolytic kidneys had increased weight and glomerular diameter, but the diameter of cell nuclei decreased. Basement membrane thickness and glomerular cell numbers did not differ between 0-hour control and autolytic kidneys. Kidneys from 4 diseased cats had increased glomerular diameter and glomerular basement membrane changes characterized by hyalin thickening and dense deposits. These changes are compatible with a lesion diagnosis of membranous glomerulonephritis.", "contents": "Feline glomeruli: morphologic comparisons in normal, autolytic, and diseased kidneys. Twelve cats were used to study autolytic changes in glomerular morphology and compare these with lesions of naturally occurring feline renal disease. The 12 cats had normal clinical, urinary, and blood features. One kidney (0-hour control) was excised immediately after a given cat was euthanatized, and portions of it were prepared for light and electron microscopy. The opposite kidney (autolytic) remained in situ for selected postmortem intervals, up to 24 hours, at which time it was similarly processed. Renal tissues from 4 additional cats (3 with proteinuria and 1 with diabetes mellitus) were processed and examined for comparison. Zero-hour control kidneys had the following mean quantitations: renal weight was 9.9 g; glomerular diameter, 83 mum; number of cells per glomerulus in 1-mum section was 63; and diameter of cell nuclei was 6.3 mum for mesangial, 6.7 mum for visceral epithelial, and 6.4 mum for endothelial. In comparison with 0-hour control kidneys, autolytic kidneys had increased weight and glomerular diameter, but the diameter of cell nuclei decreased. Basement membrane thickness and glomerular cell numbers did not differ between 0-hour control and autolytic kidneys. Kidneys from 4 diseased cats had increased glomerular diameter and glomerular basement membrane changes characterized by hyalin thickening and dense deposits. These changes are compatible with a lesion diagnosis of membranous glomerulonephritis.", "PMID": 962208}
{"id": "pubmed23n0032_4879", "title": "Ultrastructural changes in skeletal muscle of selenium-vitamin E-deficient chicks.", "content": "Chicks fed a semisynthetic basal diet deficient in selenium and vitamin E for 14 to 22 days developed skeletal myodegeneration and exudative diathesis. Chicks fed the basal diet supplemented with either 0.2 ppm of selenium (as selenite) or 100 IU alpha-tocopherol acetate/kg were protected from deficiency disease, but chicks fed the basal diet plus 0.4% L-cystine were not protected. Pectoral muscles of deficient chicks were red and edematous. Light and electron microscopic study of affected muscles revealed fibers with hyaline and granular degeneration. In hyalinized fibers, the initial ultrastructural alterations were increased density of the sarcoplasm and myofibrils, dilatation of sarcoplasmic reticulum, formation of subsarcolemmal vacuoles, and disruption of mitochondrial membranes. In later stages, alterations in these fibers included myofibrillar disruption and lysis, nuclear pyknosis and lysis, disruption of the plasma membrane with persistence of basal lamina and scattered adhering satellite cells, and eventual invasion by macrophages. In fibers with granular degeneration, the ultrastructural observations included decreased density of the sarcoplasm, prominent mitochondrial swelling and distortion, and multiple foci of myofibrillar lysis that eventually coalesced to produce generalized lysis. Prominent vascular lesions associated with exudative diathesis were present in degenerated muscle but were not considered to precede development of fiber alterations. Affected blood vessels had endothelial cells with mitochondrial damage and accumulations of cytoplasmic dense bodies and areas of endothelial disruption with adhering fibrin thrombi.", "contents": "Ultrastructural changes in skeletal muscle of selenium-vitamin E-deficient chicks. Chicks fed a semisynthetic basal diet deficient in selenium and vitamin E for 14 to 22 days developed skeletal myodegeneration and exudative diathesis. Chicks fed the basal diet supplemented with either 0.2 ppm of selenium (as selenite) or 100 IU alpha-tocopherol acetate/kg were protected from deficiency disease, but chicks fed the basal diet plus 0.4% L-cystine were not protected. Pectoral muscles of deficient chicks were red and edematous. Light and electron microscopic study of affected muscles revealed fibers with hyaline and granular degeneration. In hyalinized fibers, the initial ultrastructural alterations were increased density of the sarcoplasm and myofibrils, dilatation of sarcoplasmic reticulum, formation of subsarcolemmal vacuoles, and disruption of mitochondrial membranes. In later stages, alterations in these fibers included myofibrillar disruption and lysis, nuclear pyknosis and lysis, disruption of the plasma membrane with persistence of basal lamina and scattered adhering satellite cells, and eventual invasion by macrophages. In fibers with granular degeneration, the ultrastructural observations included decreased density of the sarcoplasm, prominent mitochondrial swelling and distortion, and multiple foci of myofibrillar lysis that eventually coalesced to produce generalized lysis. Prominent vascular lesions associated with exudative diathesis were present in degenerated muscle but were not considered to precede development of fiber alterations. Affected blood vessels had endothelial cells with mitochondrial damage and accumulations of cytoplasmic dense bodies and areas of endothelial disruption with adhering fibrin thrombi.", "PMID": 962209}
{"id": "pubmed23n0032_4880", "title": "Age-dependent resistance of chickens to salmonella in vitro: phagocytic and bactericidal activities of splenic phagocytes.", "content": "The phagocytic and bactericidal activities to Salmonella pullorum (strain 9-25) or Salmonella senftenberg (strain 99D) were examined in chicken splenic phagocytes from 0-day-old to 2-month-old chickens. The phagocytic activity against S pullorum increased in splenic phagocytes from chickens older than 7 days, but significant changes in activity against S senftenberg were not observed during the experimental period. The bactericidal activity of splenic phagocytes against S senftenberg was higher than that of phagocytes against S pullorum during the same period. Increase of the bactericidal activity against S pullorum was observed with increasing age, but the activity of the splenic phagocytes from 0-day-old chickens against S senftenberg was similar to that of the phagocytes from 2-month-old chickens. Although delayed hypersensitivity was confirmed by delayed wattle reaction in 2-month-old chickens sensitized with living S pullorum, the sensitization did not markedly affect phagocytic and bactericidal activities.", "contents": "Age-dependent resistance of chickens to salmonella in vitro: phagocytic and bactericidal activities of splenic phagocytes. The phagocytic and bactericidal activities to Salmonella pullorum (strain 9-25) or Salmonella senftenberg (strain 99D) were examined in chicken splenic phagocytes from 0-day-old to 2-month-old chickens. The phagocytic activity against S pullorum increased in splenic phagocytes from chickens older than 7 days, but significant changes in activity against S senftenberg were not observed during the experimental period. The bactericidal activity of splenic phagocytes against S senftenberg was higher than that of phagocytes against S pullorum during the same period. Increase of the bactericidal activity against S pullorum was observed with increasing age, but the activity of the splenic phagocytes from 0-day-old chickens against S senftenberg was similar to that of the phagocytes from 2-month-old chickens. Although delayed hypersensitivity was confirmed by delayed wattle reaction in 2-month-old chickens sensitized with living S pullorum, the sensitization did not markedly affect phagocytic and bactericidal activities.", "PMID": 962210}
{"id": "pubmed23n0032_4881", "title": "Effect of diethylcarbamazine on indexes of fertility in the male dog.", "content": "Four nondescript male dogs were given diethylcarbamazine citrate for 6 months at the rate of 13.2 mg/kg/day, twice the daily dose recommended for prevention of heartworm infections; 4 nonmedicated dogs were sham controls. Semen was collected and evaluated weekly or monthly for 6 months. There was no statistically significant deterioration in quantity, morphology, motility, or viability of sperm from the medicated dogs as compared with that of the controls.", "contents": "Effect of diethylcarbamazine on indexes of fertility in the male dog. Four nondescript male dogs were given diethylcarbamazine citrate for 6 months at the rate of 13.2 mg/kg/day, twice the daily dose recommended for prevention of heartworm infections; 4 nonmedicated dogs were sham controls. Semen was collected and evaluated weekly or monthly for 6 months. There was no statistically significant deterioration in quantity, morphology, motility, or viability of sperm from the medicated dogs as compared with that of the controls.", "PMID": 962211}
{"id": "pubmed23n0032_4882", "title": "Cerebrospinal fluid creatine phosphokinase in the normal dog.", "content": "Sixty-seven cerebrospinal fluid samples obtained from 44 healthy dogs were assayed for creatine phosphokinase enzyme activity. All samples contained 1 Sigma unit or less of creatine phosphokinase. Temporal variability within individuals was minimal. The biological characteristics and potential use of this enzyme in neurologic disease are discussed.", "contents": "Cerebrospinal fluid creatine phosphokinase in the normal dog. Sixty-seven cerebrospinal fluid samples obtained from 44 healthy dogs were assayed for creatine phosphokinase enzyme activity. All samples contained 1 Sigma unit or less of creatine phosphokinase. Temporal variability within individuals was minimal. The biological characteristics and potential use of this enzyme in neurologic disease are discussed.", "PMID": 962212}
{"id": "pubmed23n0032_4883", "title": "Serum cortisol (hydrocortisone) values in normal dogs as determined by radioimmunoassay.", "content": "A radioimmunoassay method was utilized to determine serum cortisol (hydrocortisone) concentrations in normal dogs. The mean value was 2.32 mug/dl (minimum, 0.94; maximum, 3.70; with a coefficient of variation of 29.7%). Samples had been collected in the morning of the diurnal cycle. The cortisol antibody used was highly specific for cortisol and showed little cross reaction with other common physiologic corticosteroids, when they were assayed, using the radioimmunoassay procedure described.", "contents": "Serum cortisol (hydrocortisone) values in normal dogs as determined by radioimmunoassay. A radioimmunoassay method was utilized to determine serum cortisol (hydrocortisone) concentrations in normal dogs. The mean value was 2.32 mug/dl (minimum, 0.94; maximum, 3.70; with a coefficient of variation of 29.7%). Samples had been collected in the morning of the diurnal cycle. The cortisol antibody used was highly specific for cortisol and showed little cross reaction with other common physiologic corticosteroids, when they were assayed, using the radioimmunoassay procedure described.", "PMID": 962213}
{"id": "pubmed23n0032_4884", "title": "Pharmacology of procaine in the horse: a preliminary report.", "content": "Rapid intravenous injection of 1 g of procaine hydrochloride in Thoroughbred mares produced variable signs of central nervous system excitation for as long as 4 minutes. Plasma concentrations of procaine were similarly variable and transient, decreasing with a half-life of approximately 25 minutes. In vitro, plasma from freshly collected equine blood hydrolyzed procaine with a half-life of approximately 7.5 minutes. This hydrolysis was apparently due to plasma esterases. Penicillin, when added free or complexed as procaine-penicillin, did not protect procaine against hydrolysis by these plasma esterases at pH 7.4.", "contents": "Pharmacology of procaine in the horse: a preliminary report. Rapid intravenous injection of 1 g of procaine hydrochloride in Thoroughbred mares produced variable signs of central nervous system excitation for as long as 4 minutes. Plasma concentrations of procaine were similarly variable and transient, decreasing with a half-life of approximately 25 minutes. In vitro, plasma from freshly collected equine blood hydrolyzed procaine with a half-life of approximately 7.5 minutes. This hydrolysis was apparently due to plasma esterases. Penicillin, when added free or complexed as procaine-penicillin, did not protect procaine against hydrolysis by these plasma esterases at pH 7.4.", "PMID": 962214}
{"id": "pubmed23n0032_4885", "title": "Susceptibility of the Mongolian gerbil (Meriones unguiculatus) to Mycobacterium paratuberculosis.", "content": "Mongolian gerbils (Meriones unguiculatus) were exposed to Mycobacterium paratuberculosis by intraperitoneal and oral routes. Fourteen weeks after exposure, the gerbils were necropsied and tissues were cultured and examined microscopically. Results of the study showed that gerbils can be infected with M paratuberculosis but are not suitable for studies of paratuberculosis.", "contents": "Susceptibility of the Mongolian gerbil (Meriones unguiculatus) to Mycobacterium paratuberculosis. Mongolian gerbils (Meriones unguiculatus) were exposed to Mycobacterium paratuberculosis by intraperitoneal and oral routes. Fourteen weeks after exposure, the gerbils were necropsied and tissues were cultured and examined microscopically. Results of the study showed that gerbils can be infected with M paratuberculosis but are not suitable for studies of paratuberculosis.", "PMID": 962215}
{"id": "pubmed23n0032_4886", "title": "Esculin hydrolysis by Streptococcus dysgalactiae.", "content": "In a mastitis control program based on eradication of intramammary infection, it is important to differentiate streptococci. According to earlier reports, Streptococcus dysgalactiae was unable to hydrolyze esculin. Preliminary data from the present study indicated that under optimal conditions, some strains of S dysgalactiae were capable of esculin hydrolysis. In a study of 45 strains of S dysgalactiae, 55.5% were able to hydrolyze esculin. In the past, these organisms would probably have been identified as Streptococcus uberis. Streptococcus dysgalactiae is more sensitive to antibiotics than S uberis, and the infection is easier to eradicate from infected glands.", "contents": "Esculin hydrolysis by Streptococcus dysgalactiae. In a mastitis control program based on eradication of intramammary infection, it is important to differentiate streptococci. According to earlier reports, Streptococcus dysgalactiae was unable to hydrolyze esculin. Preliminary data from the present study indicated that under optimal conditions, some strains of S dysgalactiae were capable of esculin hydrolysis. In a study of 45 strains of S dysgalactiae, 55.5% were able to hydrolyze esculin. In the past, these organisms would probably have been identified as Streptococcus uberis. Streptococcus dysgalactiae is more sensitive to antibiotics than S uberis, and the infection is easier to eradicate from infected glands.", "PMID": 962216}
{"id": "pubmed23n0032_4887", "title": "Extracellular antigens of Moraxella bovis.", "content": "The extracellular antigens of 2 isolates of Moraxella bovis were isolated by ammonium sulfate precipitation of cell-free culture filtrate, purified by filtration and differentiated by immunodiffusion and immunoelectrophoretic techniques. The extracellular filtrate from rough types had 2 specific extracellular antigens in addition to those in the culture filtrate of smooth-type cells of the same isolate. The extracellular antigens produced by smooth-type cells were identical in all isolates of Moraxella bovis. The serotype-specific extracellular antigens of 2 isolates were serologically identical but were different from those of a 3rd isolate. The possibility of serotyping M bovis isolates, on the basis of their extracellular rough-type antigens, was suggested. Although both serotype-specific antigens were destroyed by trypsin treatment, 1 antigen was also heat-labile and partially destroyed by formalin treatment.", "contents": "Extracellular antigens of Moraxella bovis. The extracellular antigens of 2 isolates of Moraxella bovis were isolated by ammonium sulfate precipitation of cell-free culture filtrate, purified by filtration and differentiated by immunodiffusion and immunoelectrophoretic techniques. The extracellular filtrate from rough types had 2 specific extracellular antigens in addition to those in the culture filtrate of smooth-type cells of the same isolate. The extracellular antigens produced by smooth-type cells were identical in all isolates of Moraxella bovis. The serotype-specific extracellular antigens of 2 isolates were serologically identical but were different from those of a 3rd isolate. The possibility of serotyping M bovis isolates, on the basis of their extracellular rough-type antigens, was suggested. Although both serotype-specific antigens were destroyed by trypsin treatment, 1 antigen was also heat-labile and partially destroyed by formalin treatment.", "PMID": 962217}
{"id": "pubmed23n0032_4888", "title": "Treadmill stress tests as indicators of presence and severity of coronary artery disease.", "content": "The configuration, time of onset, and duration of depressed ST segments during and after treadmill exercise testing were evaluated in 269 patients with angiographically proven coronary artery disease and 141 normal subjects. The test specificity was 93% and sensitivity 64%, the latter being influenced by the type of ST response; false-positive responses were rare with depressed, downsloping STs (1 of 123, 1%), occurred more frequently with horizontal ST depression (9 of 60, 15%), and occurred commonly with slowly upsloping STs (15 of 47, 32%). Depressed downsloping STs, ischemic changes appearing in the first 3 minutes of exercise, and those persisting past 8 minutes in recovery were associated with 91%, 86%, and 90% prevalences of two- to three-vessel or main left coronary disease, respectively. It is concluded that attention to configuration, time of onset, and duration of ischemic ST depression aids both in assessing the validity of exercise responses in diagnosing coronary artery disease and in delineating patients with advanced coronary obstruction.", "contents": "Treadmill stress tests as indicators of presence and severity of coronary artery disease. The configuration, time of onset, and duration of depressed ST segments during and after treadmill exercise testing were evaluated in 269 patients with angiographically proven coronary artery disease and 141 normal subjects. The test specificity was 93% and sensitivity 64%, the latter being influenced by the type of ST response; false-positive responses were rare with depressed, downsloping STs (1 of 123, 1%), occurred more frequently with horizontal ST depression (9 of 60, 15%), and occurred commonly with slowly upsloping STs (15 of 47, 32%). Depressed downsloping STs, ischemic changes appearing in the first 3 minutes of exercise, and those persisting past 8 minutes in recovery were associated with 91%, 86%, and 90% prevalences of two- to three-vessel or main left coronary disease, respectively. It is concluded that attention to configuration, time of onset, and duration of ischemic ST depression aids both in assessing the validity of exercise responses in diagnosing coronary artery disease and in delineating patients with advanced coronary obstruction.", "PMID": 962219}
{"id": "pubmed23n0032_4889", "title": "Aspirin-induced ultrastructural changes in human gastric mucosa: correlation with potential difference.", "content": "Aspirin breaks the gastric mucosal barrier. We studied the effect of aspirin on this barrier, correlating changes in potential difference and ultrastructure. Mean control potential difference for seven subjects was -48 +/- 1.0 mV. Oral aspirin, 600 mg in 100 ml of saline, reduced potential difference to -39 +/- 1.4 mV (P less than 0.001) within 10 minutes. Gastric biopsies were taken during control, aspirin- instillation, and recovery periods. Damage was present in all biopsies after aspirin. Light microscopy showed focal cell disruption, loss of mucous granules, and apical membrane rupture. Transmission electron microscopy showed intact tight junctions. Scanning electron microscopy showed loss of normal cobblestone cell apices, giving a honeycombed surface. Ten minutes after aspirin, 25% of surface epithelial cells were damaged. Marked recovery was noted at 1 hr, with a normal potential difference and only 9% cell damage. We conclude that single \"routine\" doses of aspirin cause focal damage to normal human gastric mucosa.", "contents": "Aspirin-induced ultrastructural changes in human gastric mucosa: correlation with potential difference. Aspirin breaks the gastric mucosal barrier. We studied the effect of aspirin on this barrier, correlating changes in potential difference and ultrastructure. Mean control potential difference for seven subjects was -48 +/- 1.0 mV. Oral aspirin, 600 mg in 100 ml of saline, reduced potential difference to -39 +/- 1.4 mV (P less than 0.001) within 10 minutes. Gastric biopsies were taken during control, aspirin- instillation, and recovery periods. Damage was present in all biopsies after aspirin. Light microscopy showed focal cell disruption, loss of mucous granules, and apical membrane rupture. Transmission electron microscopy showed intact tight junctions. Scanning electron microscopy showed loss of normal cobblestone cell apices, giving a honeycombed surface. Ten minutes after aspirin, 25% of surface epithelial cells were damaged. Marked recovery was noted at 1 hr, with a normal potential difference and only 9% cell damage. We conclude that single \"routine\" doses of aspirin cause focal damage to normal human gastric mucosa.", "PMID": 962220}
{"id": "pubmed23n0032_4890", "title": "Renal vein thrombosis, nephrotic syndrome, and systemic lupus erythematosus: an association in four cases.", "content": "We report here four cases of systemic lupus erythematosus associated with the nephrotic syndrome and renal vein thrombosis and review six familiar cases previously noted in the literature. Renal biopsy in each of our cases showed changes consistent with the membranous type of lupus nephropathy. We discuss the exclusive appearance of this pattern in relation to renal vein thrombosis in other forms of renal disease. The occurance of renal vein thrombosis in patients with systemic lupus erythematosus and the nephrotic syndrome supports the evidence that the thrombosis is a complication rather than a cause of the nephrotic syndrome. The presence of pleuritic pain in a patient with systemic lupus erythematosus and the nephrotic syndrome should alert the clinician to the possibility of renal vein thrombosis and pulmonary emboli. With appropriate diagnosis and anticoagulation therapy, our patients had a benign course during 7 to 48 months of follow-up.", "contents": "Renal vein thrombosis, nephrotic syndrome, and systemic lupus erythematosus: an association in four cases. We report here four cases of systemic lupus erythematosus associated with the nephrotic syndrome and renal vein thrombosis and review six familiar cases previously noted in the literature. Renal biopsy in each of our cases showed changes consistent with the membranous type of lupus nephropathy. We discuss the exclusive appearance of this pattern in relation to renal vein thrombosis in other forms of renal disease. The occurance of renal vein thrombosis in patients with systemic lupus erythematosus and the nephrotic syndrome supports the evidence that the thrombosis is a complication rather than a cause of the nephrotic syndrome. The presence of pleuritic pain in a patient with systemic lupus erythematosus and the nephrotic syndrome should alert the clinician to the possibility of renal vein thrombosis and pulmonary emboli. With appropriate diagnosis and anticoagulation therapy, our patients had a benign course during 7 to 48 months of follow-up.", "PMID": 962221}
{"id": "pubmed23n0032_4891", "title": "Erythrocyte filterability and heme catabolism during the menstrual cycle.", "content": "Erythrocyte filterability, reticulocyte count, total serum bilirubin, and CO hemoglobin percent saturation (COHb) were measured throughout one menstrual cycle in 17 women. The filterability was significantly depressed during the progesterone phase compared with the estrogen phase. The depressed filterability was accompanied  by significant increases in reticulocyte count, total serum bilirubin, and COHb. Significant correlations were seen between filterability, reticulocyte count, and total serum bilirubin. However, COHb did not correlate significantly with any of the other variables studied. This is probably due to increased ventilation during the progesterone phase balancing the increased endogenous production of CO. It is possible that cyclic variations in heme turnover are related to changes in erythrocyte characteristics during the progesterone phase.", "contents": "Erythrocyte filterability and heme catabolism during the menstrual cycle. Erythrocyte filterability, reticulocyte count, total serum bilirubin, and CO hemoglobin percent saturation (COHb) were measured throughout one menstrual cycle in 17 women. The filterability was significantly depressed during the progesterone phase compared with the estrogen phase. The depressed filterability was accompanied  by significant increases in reticulocyte count, total serum bilirubin, and COHb. Significant correlations were seen between filterability, reticulocyte count, and total serum bilirubin. However, COHb did not correlate significantly with any of the other variables studied. This is probably due to increased ventilation during the progesterone phase balancing the increased endogenous production of CO. It is possible that cyclic variations in heme turnover are related to changes in erythrocyte characteristics during the progesterone phase.", "PMID": 962222}
{"id": "pubmed23n0032_4892", "title": "Reversible restrictive lung disease stimulating asthma.", "content": "A young adult woman presented with spontaneous and exercise-induced episodic dyspnea without wheezing. During dyspneic episodes all static lung volumes decreased markedly, the inspiratory capacity fell from 3.0 to 1.1 litres and total lung capacity from 5.3 to 2.6 litres. Airway resistance (Raw) remained normal, and maximal expiratory flows at low absolute lung volume actually increased. Static compliance decreased from 0.153 to 0.077 litre/cmH2O, and the elastic recoil increased from 2.8 to 4.8 cmH2O at 50% of baseline total lung capacity when dyspneic. Upstream airway resistance (Rus) remained unchanged, 1.7 cmH2O/litre/sec. All physiologic variables indicated a reversible restrictive process exists, the origin of which is unclear. It is concluded that the episodic increase in elastic recoil properties results in [1] loss of volume primarily in the alveolar ducts and sacs, [2] preservation of normal Rus by an increase in driving pressure, and [3] maintenance of normal Raw by stabilization of larger airways.", "contents": "Reversible restrictive lung disease stimulating asthma. A young adult woman presented with spontaneous and exercise-induced episodic dyspnea without wheezing. During dyspneic episodes all static lung volumes decreased markedly, the inspiratory capacity fell from 3.0 to 1.1 litres and total lung capacity from 5.3 to 2.6 litres. Airway resistance (Raw) remained normal, and maximal expiratory flows at low absolute lung volume actually increased. Static compliance decreased from 0.153 to 0.077 litre/cmH2O, and the elastic recoil increased from 2.8 to 4.8 cmH2O at 50% of baseline total lung capacity when dyspneic. Upstream airway resistance (Rus) remained unchanged, 1.7 cmH2O/litre/sec. All physiologic variables indicated a reversible restrictive process exists, the origin of which is unclear. It is concluded that the episodic increase in elastic recoil properties results in [1] loss of volume primarily in the alveolar ducts and sacs, [2] preservation of normal Rus by an increase in driving pressure, and [3] maintenance of normal Raw by stabilization of larger airways.", "PMID": 962223}
{"id": "pubmed23n0032_4893", "title": "Medicine and mass communication: an agenda for physicians.", "content": "Because the medical content of the mass media significantly affects public attitudes and behavior, physicians should participate more fully as critics and sources of that content. Attempts to sway audiences with medical information alone are often unsuccessful. Communication is more likely to be effective when it offers people something they want, such as satisfaction of a need or legitimation of an existing value. Except for advertising, the media seldom engineer their impact intentionally. Instead, the producers of news and entertainment rely heavily on sources, critics, and pressure groups outside the communications industry. By mastering the patterns of media impact and the rudiments of public relations, physicians can make the media a useful tool of medicine.", "contents": "Medicine and mass communication: an agenda for physicians. Because the medical content of the mass media significantly affects public attitudes and behavior, physicians should participate more fully as critics and sources of that content. Attempts to sway audiences with medical information alone are often unsuccessful. Communication is more likely to be effective when it offers people something they want, such as satisfaction of a need or legitimation of an existing value. Except for advertising, the media seldom engineer their impact intentionally. Instead, the producers of news and entertainment rely heavily on sources, critics, and pressure groups outside the communications industry. By mastering the patterns of media impact and the rudiments of public relations, physicians can make the media a useful tool of medicine.", "PMID": 962225}
{"id": "pubmed23n0032_4894", "title": "[2 cases of post-radiation stenosis of the main cerebral artery trunks].", "content": "Two cases of common carotid stenosis as a late complication of radiotherapy are reported. One of them was treated by subclavian-carotid by-pass. Delayed lesions of medium and large arteries after radiotherapy are reviewed, with emphasis on the neck vessels--and the surgical possibilities.", "contents": "[2 cases of post-radiation stenosis of the main cerebral artery trunks]. Two cases of common carotid stenosis as a late complication of radiotherapy are reported. One of them was treated by subclavian-carotid by-pass. Delayed lesions of medium and large arteries after radiotherapy are reviewed, with emphasis on the neck vessels--and the surgical possibilities.", "PMID": 962239}
{"id": "pubmed23n0032_4895", "title": "[Disorders of intracardiac conduction during ankylosing spondylarthritis. Apropos of 19 cases].", "content": "The authors have collected 19 cases of ankylosing spondylitis with an alteration of intracardiac conduction. The lesions are usually situated high in the bundle of His, as shown by successive electrocardiograms and endocavitary studies. Progression by regressive acute episodes and the response to anti-inflammatory agents suggests that the disorder of conduction is inflammatory in origin. Syncopal attacks are rare (1 case out of 19) and a pacemaker is rarely indicated. Almost half the patients had aortic insufficiency, one patient had tricuspid stenosis and two patients had heart failure in the absence of any valvular lesion. The associated ankylosing spondylitis is characterised by the severity of the inflammatory signs (average sedimentation rate 50 mm in the first hour) and by the extent of peripheral articular involvement and extra-rheumatological manifestations. Almost one in two patients had iritis, with the same proportion applying to a past history of Reiters syndrome.", "contents": "[Disorders of intracardiac conduction during ankylosing spondylarthritis. Apropos of 19 cases]. The authors have collected 19 cases of ankylosing spondylitis with an alteration of intracardiac conduction. The lesions are usually situated high in the bundle of His, as shown by successive electrocardiograms and endocavitary studies. Progression by regressive acute episodes and the response to anti-inflammatory agents suggests that the disorder of conduction is inflammatory in origin. Syncopal attacks are rare (1 case out of 19) and a pacemaker is rarely indicated. Almost half the patients had aortic insufficiency, one patient had tricuspid stenosis and two patients had heart failure in the absence of any valvular lesion. The associated ankylosing spondylitis is characterised by the severity of the inflammatory signs (average sedimentation rate 50 mm in the first hour) and by the extent of peripheral articular involvement and extra-rheumatological manifestations. Almost one in two patients had iritis, with the same proportion applying to a past history of Reiters syndrome.", "PMID": 962240}
{"id": "pubmed23n0032_4896", "title": "[Spontaneous adrenal hemorrhage. Apropos of a case].", "content": "Spontaneous adrenal haemorrhages are rare but non exceptional. They mainly occur in elderly men (mean age: 61 years). The main risk factors are: anticoagulant therapy (prescribed for myocardial infarction, occlusive vascular ou thromboembolic disease), hypertension and chronic respiratory insufficiency, as in the present case. The clinical picture is one of abdominal of lumbar pain with abdominal distension, circulatory collapse, often delayed and sometimes preceeded by an hypertensive bout, quite often fever with leucocytosis. Hyponatraemia and hyperkalaemia, are not constant features. The diagnosis is often not made until laparotomy, and it even can be missed there. In fact, plasma cortisol level or, if unavailable, urinary corticosteroid measurements are the best diagnostic procedures, permitting adequate therapy and giving best chances of survival. They must always be made in a patient with abdominal signs falling into one of the above mentioned etiologic categories.", "contents": "[Spontaneous adrenal hemorrhage. Apropos of a case]. Spontaneous adrenal haemorrhages are rare but non exceptional. They mainly occur in elderly men (mean age: 61 years). The main risk factors are: anticoagulant therapy (prescribed for myocardial infarction, occlusive vascular ou thromboembolic disease), hypertension and chronic respiratory insufficiency, as in the present case. The clinical picture is one of abdominal of lumbar pain with abdominal distension, circulatory collapse, often delayed and sometimes preceeded by an hypertensive bout, quite often fever with leucocytosis. Hyponatraemia and hyperkalaemia, are not constant features. The diagnosis is often not made until laparotomy, and it even can be missed there. In fact, plasma cortisol level or, if unavailable, urinary corticosteroid measurements are the best diagnostic procedures, permitting adequate therapy and giving best chances of survival. They must always be made in a patient with abdominal signs falling into one of the above mentioned etiologic categories.", "PMID": 962241}
{"id": "pubmed23n0032_4897", "title": "[Renal manifestations of Hodgkin's disease].", "content": "The occurrence of renal manifestations of Hodgkin's disease may result from multiple physio-pathological processes: mechanical, by obstruction of the vessels or excretory systems of the kidneys; specific, by lymphogranulomatous infiltration of the parenchyma; immunological, as immunune complex glomerulonephritis or, more frequently, as amyloid disease; infective or metabolic as a complication of long term corticosteroid or immunodepressive therapy. These various causes of renal problems during Hodgkin's disease lead to the observation of three principal clinical pictures: acute renal failure, chronic renal failure and permanent proteinuria with or without nephrotic syndrome. Acute renal failure generally results from a severe infection with toxi-infective shock. More rarely it is related to thrombosis of the renal veins, with a grave prognosis, or to unreteral compression with anatomical or functional exclusion of the contralateral kidney. Chronic renal failure may be caused either by distension of the excretory pathways, progressively obstructed or invaded by the Hodgkin's process and requiring specific therapy to relieve the obstruction (cobaltotherapy, chemotherapy), by specific infiltration of the renal parenchyma or by amyloid disease. Permanent proteinuria, with or without nephrotic syndrome, may be the presenting feature of renal vein thrombosis, amyloidosis or paraneoplastic nephrotic syndrome.", "contents": "[Renal manifestations of Hodgkin's disease]. The occurrence of renal manifestations of Hodgkin's disease may result from multiple physio-pathological processes: mechanical, by obstruction of the vessels or excretory systems of the kidneys; specific, by lymphogranulomatous infiltration of the parenchyma; immunological, as immunune complex glomerulonephritis or, more frequently, as amyloid disease; infective or metabolic as a complication of long term corticosteroid or immunodepressive therapy. These various causes of renal problems during Hodgkin's disease lead to the observation of three principal clinical pictures: acute renal failure, chronic renal failure and permanent proteinuria with or without nephrotic syndrome. Acute renal failure generally results from a severe infection with toxi-infective shock. More rarely it is related to thrombosis of the renal veins, with a grave prognosis, or to unreteral compression with anatomical or functional exclusion of the contralateral kidney. Chronic renal failure may be caused either by distension of the excretory pathways, progressively obstructed or invaded by the Hodgkin's process and requiring specific therapy to relieve the obstruction (cobaltotherapy, chemotherapy), by specific infiltration of the renal parenchyma or by amyloid disease. Permanent proteinuria, with or without nephrotic syndrome, may be the presenting feature of renal vein thrombosis, amyloidosis or paraneoplastic nephrotic syndrome.", "PMID": 962242}
{"id": "pubmed23n0032_4898", "title": "Quantification of intraocular malignant melanoma volume: a preliminary report.", "content": "Intraocular malignant melanoma size is a significant prognostic indicator. No accurate volume determining technique has been generally utilized. Plastic models and selected geometric formulae were used to demonstrate a technique for volume calculation of intraocular malignant melanomas. The accuracy of geometric analysis is shown to be superior to previous \"size\" calculations. We believe this increased accuracy would be useful in following patients for tumor growth utilizing A and B scan ultrasonography as well as in quantifying tumor volumes in the histopathology laboratory.", "contents": "Quantification of intraocular malignant melanoma volume: a preliminary report. Intraocular malignant melanoma size is a significant prognostic indicator. No accurate volume determining technique has been generally utilized. Plastic models and selected geometric formulae were used to demonstrate a technique for volume calculation of intraocular malignant melanomas. The accuracy of geometric analysis is shown to be superior to previous \"size\" calculations. We believe this increased accuracy would be useful in following patients for tumor growth utilizing A and B scan ultrasonography as well as in quantifying tumor volumes in the histopathology laboratory.", "PMID": 962255}
{"id": "pubmed23n0032_4899", "title": "Fluorescein angiography of the fundus after pars plana vitrectomy.", "content": "In diabetic patients with massive vitreous hemorrhage, fluorescein angiography after pars plana vitrectomy demonstrated varying degrees of vascular involvement depending on the stage of the basic disease process. Arteriolar occlusions, capillary bed drop-out, microaneurysms, and neovascularization were common findings. We concluded that diabetic retinopathy continued its course behind the cloudy vitreous, sometimes leading to retinal ischemia and subsequent spontaneous involution, macular disease, or retinal detachment. In addition, some of these patients were also subject to nondiabetic diseases, such as senile macular degeneration, that could not be detected preoperatively, but that reduced visual acuity postoperatively. In the nondiabetic patients with vitreous hemorrhage, visual acuity after vitrectomy usually depended on the degree of coincident macular disease.", "contents": "Fluorescein angiography of the fundus after pars plana vitrectomy. In diabetic patients with massive vitreous hemorrhage, fluorescein angiography after pars plana vitrectomy demonstrated varying degrees of vascular involvement depending on the stage of the basic disease process. Arteriolar occlusions, capillary bed drop-out, microaneurysms, and neovascularization were common findings. We concluded that diabetic retinopathy continued its course behind the cloudy vitreous, sometimes leading to retinal ischemia and subsequent spontaneous involution, macular disease, or retinal detachment. In addition, some of these patients were also subject to nondiabetic diseases, such as senile macular degeneration, that could not be detected preoperatively, but that reduced visual acuity postoperatively. In the nondiabetic patients with vitreous hemorrhage, visual acuity after vitrectomy usually depended on the degree of coincident macular disease.", "PMID": 962256}
{"id": "pubmed23n0032_4900", "title": "[Taxonomic positions of H2S-Enterobacteria in relation to the genus (Citrobacter) (author's transl)].", "content": "This work studies the classification, by numerical procedure, of 111 strains belonging to the family Enterobacteriaceae. Their taxonomic position is discussed, in relation to the genus Citrobacter, Levinea, Enterobacter, to which some appear to be closely associated. The study is based on the usual biochemical and nutritional characters as shown by the utilisation of substrates tested as sole sources of carbon and energy. Six hierarchical classes can thus be defined. Their degree of affinity with members of the Enterobacteriaceae groups or genera is discussed. The diverse origins of the strains examined (human, soil, aquatic) are certainly the cause for the individualization of the new classes.", "contents": "[Taxonomic positions of H2S-Enterobacteria in relation to the genus (Citrobacter) (author's transl)]. This work studies the classification, by numerical procedure, of 111 strains belonging to the family Enterobacteriaceae. Their taxonomic position is discussed, in relation to the genus Citrobacter, Levinea, Enterobacter, to which some appear to be closely associated. The study is based on the usual biochemical and nutritional characters as shown by the utilisation of substrates tested as sole sources of carbon and energy. Six hierarchical classes can thus be defined. Their degree of affinity with members of the Enterobacteriaceae groups or genera is discussed. The diverse origins of the strains examined (human, soil, aquatic) are certainly the cause for the individualization of the new classes.", "PMID": 962250}
{"id": "pubmed23n0032_4901", "title": "[Search of some glycosidases in 22 species of (Bacillus) (author's transl)].", "content": "Disc-method has been applied in order to bring to light three enzymes: beta-xylosidase, alpha-fucosidase, and alpha-mannosidase in 22 species of Bacillus; an unequal distribution of those enzymes which may be applied to differential diagnosis of some species was so shown.", "contents": "[Search of some glycosidases in 22 species of (Bacillus) (author's transl)]. Disc-method has been applied in order to bring to light three enzymes: beta-xylosidase, alpha-fucosidase, and alpha-mannosidase in 22 species of Bacillus; an unequal distribution of those enzymes which may be applied to differential diagnosis of some species was so shown.", "PMID": 962252}
{"id": "pubmed23n0032_4902", "title": "A study of the capacity of soils to induce nodules in Alnus glutinosa (L.) Gaertn. and Myrica gale L., with special reference to the specificity of the endophytes.", "content": "One hundred soil samples from sites in North, Centre and West of Spain were examined for their capacity to induce nodules in alder (Alnus glutinosa) and bog myrtle (Myrica gale). Soils from A. glutinosa communities particularly from places where Myrica was absent, were checked for their infectivity towards M. gale. There was a good correlation between the results, since there was not one single sample which could induce nodules in Myrica which did not do the same in alder. The nodules formed on M. gale plants grown in soils collected from alder habitats showed acetylene-reducing activity at a level comparable to that described in the literature, in the case of other Myrica species. Considering together the results now obtained and in previous work, the possibility of cross-inoculation between M. gale and A. glutinosa, is again put forward and it is thus held that no specificity can be assigned, for the time being, to the respective endophytes.", "contents": "A study of the capacity of soils to induce nodules in Alnus glutinosa (L.) Gaertn. and Myrica gale L., with special reference to the specificity of the endophytes. One hundred soil samples from sites in North, Centre and West of Spain were examined for their capacity to induce nodules in alder (Alnus glutinosa) and bog myrtle (Myrica gale). Soils from A. glutinosa communities particularly from places where Myrica was absent, were checked for their infectivity towards M. gale. There was a good correlation between the results, since there was not one single sample which could induce nodules in Myrica which did not do the same in alder. The nodules formed on M. gale plants grown in soils collected from alder habitats showed acetylene-reducing activity at a level comparable to that described in the literature, in the case of other Myrica species. Considering together the results now obtained and in previous work, the possibility of cross-inoculation between M. gale and A. glutinosa, is again put forward and it is thus held that no specificity can be assigned, for the time being, to the respective endophytes.", "PMID": 962251}
{"id": "pubmed23n0032_4903", "title": "Systemic toxicity of cyclopentolate hydrochloride in adults following topical ocular instillation.", "content": "A case reported of a 27-year-old white woman who developed systemic toxicity after the local instillation of one drop of 2% cyclopentolate solution in each eye. The usefulness and toxic reactions of this very valuable drug are discussed. It is recommended that every ophthalmologist should warn not only the parents of the children, but also the adult patients about the possibility of systemic reaction before this drug is used and where possible the lowest concentration of solution be used. It is also suggested that a sufficient amount of injectable physostigmine salicylate be kept handy in the office emergency kit.", "contents": "Systemic toxicity of cyclopentolate hydrochloride in adults following topical ocular instillation. A case reported of a 27-year-old white woman who developed systemic toxicity after the local instillation of one drop of 2% cyclopentolate solution in each eye. The usefulness and toxic reactions of this very valuable drug are discussed. It is recommended that every ophthalmologist should warn not only the parents of the children, but also the adult patients about the possibility of systemic reaction before this drug is used and where possible the lowest concentration of solution be used. It is also suggested that a sufficient amount of injectable physostigmine salicylate be kept handy in the office emergency kit.", "PMID": 962257}
{"id": "pubmed23n0032_4904", "title": "Vitreochorioretinal degeneration associated with trichomegaly.", "content": "A 15-year-old white boy had bilateral vitreochorioretinal degeneration associated with trichomegaly. Findings on electroretinography and fluorescein angiography provided additional information on the retinal changes not noted in 3 previously reported cases having these associated features. Although similar on ophthalmoscopy, fluourescein angiography, and by electroretinography, the retinal disease in our patient differed from cases of gyrate atrophy of the choroid and retina in having normal plasma ornithine levels.", "contents": "Vitreochorioretinal degeneration associated with trichomegaly. A 15-year-old white boy had bilateral vitreochorioretinal degeneration associated with trichomegaly. Findings on electroretinography and fluorescein angiography provided additional information on the retinal changes not noted in 3 previously reported cases having these associated features. Although similar on ophthalmoscopy, fluourescein angiography, and by electroretinography, the retinal disease in our patient differed from cases of gyrate atrophy of the choroid and retina in having normal plasma ornithine levels.", "PMID": 962258}
{"id": "pubmed23n0032_4905", "title": "Possibility of isoproterenol therapy with soft contact lenses: ocular hypotension without systemic effects.", "content": "An experimental investigation performed in the rabbit pointed out the possibility of administering isoproterenol at low concentrations with the aid of soft contact lenses presoaked in 0.2% isoproterenol for 60 to 120 minutes. A reduction in intraocular pressure ranging from 23% to 32% was observed in 21 out of 24 glaucomatous patients. Duration of hypotension in 50% of eyes treated was more than 8 hours. Thus, a therapeutic effect was obtained with a concentration (0.2%) approximately 50 times lower than that previously employed clinically (2.4%). No systemic side effects, such as arterial hypotension and increase in heart rate, were noted.", "contents": "Possibility of isoproterenol therapy with soft contact lenses: ocular hypotension without systemic effects. An experimental investigation performed in the rabbit pointed out the possibility of administering isoproterenol at low concentrations with the aid of soft contact lenses presoaked in 0.2% isoproterenol for 60 to 120 minutes. A reduction in intraocular pressure ranging from 23% to 32% was observed in 21 out of 24 glaucomatous patients. Duration of hypotension in 50% of eyes treated was more than 8 hours. Thus, a therapeutic effect was obtained with a concentration (0.2%) approximately 50 times lower than that previously employed clinically (2.4%). No systemic side effects, such as arterial hypotension and increase in heart rate, were noted.", "PMID": 962259}
{"id": "pubmed23n0032_4906", "title": "Angle closure glaucoma in a patient with pseudoexfoliation of the lens capsule.", "content": "Glaucoma with gonioscopically open angles is found in a variable number of patients with capsular pseudoexfoliation. A patient is described who presented with bilateral pseudoexfoliation and unilateral acute angle closure glaucoma. Previously the association of these two clinical entities was thought to be coincidental. Certain characteristics of the present case suggest that pseudoexfoliation may occasionally be casually related to acute angle closure, possibly through the production of a pupillary block phenomenon.", "contents": "Angle closure glaucoma in a patient with pseudoexfoliation of the lens capsule. Glaucoma with gonioscopically open angles is found in a variable number of patients with capsular pseudoexfoliation. A patient is described who presented with bilateral pseudoexfoliation and unilateral acute angle closure glaucoma. Previously the association of these two clinical entities was thought to be coincidental. Certain characteristics of the present case suggest that pseudoexfoliation may occasionally be casually related to acute angle closure, possibly through the production of a pupillary block phenomenon.", "PMID": 962262}
{"id": "pubmed23n0032_4907", "title": "Causes for removal of the eye: a study of 890 eyes.", "content": "This study is a review of 890 cases of eye enucleations performed over a period of 22 years. The major cause for enucleations was trauma, accounting for 39.7% of the eyes enucleated. Chronic inflammatory diseases accounted for 22.1%, glaucoma for 11.5% and tumours for 9.2% in this group. Retinal diseases were responsible for 5.5% of the eyes; vascular occlusion for 5%; and miscellaneous causes for the remaining 7%. Injuries were everyday tools and small flying missiles caused most of the trauma. Previously planned surgical operations accounted for 15% of the enucleations. Forty-eight of these eyes had epithelial or fibrous downgrowths resulting from inflammation, secondary glaucoma, and phthisis bulbi. A more careful selection of cases and better surgical techniques might significantly lower these statistics. Also, vascular occlusion should be given a high level of suspicion in all intractable cases of glaucoma of unexplained etiology. As a general rule, enucleations should not be performed because of the suspected presence of sympathetic ophthalmia.", "contents": "Causes for removal of the eye: a study of 890 eyes. This study is a review of 890 cases of eye enucleations performed over a period of 22 years. The major cause for enucleations was trauma, accounting for 39.7% of the eyes enucleated. Chronic inflammatory diseases accounted for 22.1%, glaucoma for 11.5% and tumours for 9.2% in this group. Retinal diseases were responsible for 5.5% of the eyes; vascular occlusion for 5%; and miscellaneous causes for the remaining 7%. Injuries were everyday tools and small flying missiles caused most of the trauma. Previously planned surgical operations accounted for 15% of the enucleations. Forty-eight of these eyes had epithelial or fibrous downgrowths resulting from inflammation, secondary glaucoma, and phthisis bulbi. A more careful selection of cases and better surgical techniques might significantly lower these statistics. Also, vascular occlusion should be given a high level of suspicion in all intractable cases of glaucoma of unexplained etiology. As a general rule, enucleations should not be performed because of the suspected presence of sympathetic ophthalmia.", "PMID": 962264}
{"id": "pubmed23n0032_4908", "title": "Vascular pseudopapillitis: ischemic optic neuropathy.", "content": "There are two acute ischemic processes of the optic nerve. Vascular pseudopapillitis or ischemic optic neuropathy is characterized by a palid papilledema, followed by an optic atrophy, by a reduction of the visual acuity and fascicular defects of the visual field. Arteritis temporalis is characterized by the same ophthalmologic signs and by a raised erythrocyte sedimentation rate. Histologic examination of the temporal artery permits not only the diagnosis, but also the differentiation between the two diseases, which are due to an involvement of the short posterior ciliary arteries or of the central vascular system or pial system of the optic nerve head.", "contents": "Vascular pseudopapillitis: ischemic optic neuropathy. There are two acute ischemic processes of the optic nerve. Vascular pseudopapillitis or ischemic optic neuropathy is characterized by a palid papilledema, followed by an optic atrophy, by a reduction of the visual acuity and fascicular defects of the visual field. Arteritis temporalis is characterized by the same ophthalmologic signs and by a raised erythrocyte sedimentation rate. Histologic examination of the temporal artery permits not only the diagnosis, but also the differentiation between the two diseases, which are due to an involvement of the short posterior ciliary arteries or of the central vascular system or pial system of the optic nerve head.", "PMID": 962265}
{"id": "pubmed23n0032_4909", "title": "Recent experiences on the ophthalmologic complications of oral contraceptives.", "content": "I have added 16 cases to the preciously reported 4 cases where ophthalmologic complications occurred in connection with oral contraceptives. In some cases, occlusion of the central retinal artery, prethrombosis of the central retinal vein, prolonged narrowing of the retinal arteries, perivasculitis, hemorrhage in the macular region, and inflammations of the anterior segment of the eye, resistance to therapy developed, and in some cases, lesions of the optic nerve causing visual loss of different degree and papillitis occurred. In the background of the different clinical entities, common pathologic origin might be supposed,--the disturbance of the chorooretinal circulation. In patients taking the pill for the longest time interval, delicate lenticular opacities developed.", "contents": "Recent experiences on the ophthalmologic complications of oral contraceptives. I have added 16 cases to the preciously reported 4 cases where ophthalmologic complications occurred in connection with oral contraceptives. In some cases, occlusion of the central retinal artery, prethrombosis of the central retinal vein, prolonged narrowing of the retinal arteries, perivasculitis, hemorrhage in the macular region, and inflammations of the anterior segment of the eye, resistance to therapy developed, and in some cases, lesions of the optic nerve causing visual loss of different degree and papillitis occurred. In the background of the different clinical entities, common pathologic origin might be supposed,--the disturbance of the chorooretinal circulation. In patients taking the pill for the longest time interval, delicate lenticular opacities developed.", "PMID": 962266}
{"id": "pubmed23n0032_4910", "title": "A new concept of the sympathetic pathways to the eye.", "content": "The sympathetic pupillociliary pathways controlling the dilatation of the pupil in man have been recorded by many authorities as passing via the first and/or second thoracic (dorsal) rami to the lower part of the stellate (first thoracic) ganglion. It has been stated by these and other authorities that the removal of the lower part of the stellate ganglion and/or resection of the first and/or second thoracic rami would produce a Horner's syndrome. This currently accepted concept of the sympathetic pathways to the eye we believe to be incorrect. Our entire clinical experience has consistently contradicted the findings and reports of other investigators. It is suggested that the ability afforded by a new surgical approach to reach, dissect, and exactly control the line of resection without undue trauma to the stellate ganglion has made possible for the first time a definitive statement concerning the entry of the pupillociliary pathways into the sympathetic chain. It is, therefore, postulated that the preganglionic neurons controlling the pupil enter the upper portion of the stellate ganglion by a separate paravertebral route leaving the ventral roots of the eighth cervical, first and/or second thoracic nerves. Our entire clinical experience refutes the concept that these pathways pass via the first ramus communicans to the first thoracic ganglion. This thesis is based on and supported by the results of new surgical approach originally designed to permit a more direct exposure and to overcome many of the deficiencies of current surgical approaches. The anterior transthoracic, transpleural wound employed allows a more direct approach and a more accurate and complete dissection of this segment of the sympathetic supply to the head, neck, upper extremity, heart, and coronary vessels without incurring the undesirable sequela of a Horner's syndrome in 93% of patients.", "contents": "A new concept of the sympathetic pathways to the eye. The sympathetic pupillociliary pathways controlling the dilatation of the pupil in man have been recorded by many authorities as passing via the first and/or second thoracic (dorsal) rami to the lower part of the stellate (first thoracic) ganglion. It has been stated by these and other authorities that the removal of the lower part of the stellate ganglion and/or resection of the first and/or second thoracic rami would produce a Horner's syndrome. This currently accepted concept of the sympathetic pathways to the eye we believe to be incorrect. Our entire clinical experience has consistently contradicted the findings and reports of other investigators. It is suggested that the ability afforded by a new surgical approach to reach, dissect, and exactly control the line of resection without undue trauma to the stellate ganglion has made possible for the first time a definitive statement concerning the entry of the pupillociliary pathways into the sympathetic chain. It is, therefore, postulated that the preganglionic neurons controlling the pupil enter the upper portion of the stellate ganglion by a separate paravertebral route leaving the ventral roots of the eighth cervical, first and/or second thoracic nerves. Our entire clinical experience refutes the concept that these pathways pass via the first ramus communicans to the first thoracic ganglion. This thesis is based on and supported by the results of new surgical approach originally designed to permit a more direct exposure and to overcome many of the deficiencies of current surgical approaches. The anterior transthoracic, transpleural wound employed allows a more direct approach and a more accurate and complete dissection of this segment of the sympathetic supply to the head, neck, upper extremity, heart, and coronary vessels without incurring the undesirable sequela of a Horner's syndrome in 93% of patients.", "PMID": 962268}
{"id": "pubmed23n0032_4911", "title": "Anterior uveitis and leptospirosis.", "content": "Seven patients with anterior uveitis due to leptospirosis are presented. The diagnosis was based upon serologic tests and exclusion of other etiologic possibilities. Six patients came from a well-known endemic area of leptospirosis during the time of epidemics and one case came from an area which was not known to be endemic at the time. In 5 patients the uveitis was bilateral. The disease was mild in all but 2 patents who needed systemic administration of steroids. The posterior part of the eye was not involved in any of the cases.", "contents": "Anterior uveitis and leptospirosis. Seven patients with anterior uveitis due to leptospirosis are presented. The diagnosis was based upon serologic tests and exclusion of other etiologic possibilities. Six patients came from a well-known endemic area of leptospirosis during the time of epidemics and one case came from an area which was not known to be endemic at the time. In 5 patients the uveitis was bilateral. The disease was mild in all but 2 patents who needed systemic administration of steroids. The posterior part of the eye was not involved in any of the cases.", "PMID": 962269}
{"id": "pubmed23n0032_4912", "title": "Eyelash tolerated in the anterior chamber and cornea.", "content": "Cilia may be carried into the eye following a penetrating injury. Such a case of a cilium in the anterior chamber is described. The cilium was discovered years later following an injury. A cilium was noted to extend through the cornea into the anterior chamber barely touching the anterior lens capsule. Although the lens surface directly directly adjacent to the foreign body was clear, an anterior capsule lens opacification and area of posterior syncechia were noted at the 3 o'clock meridian. The eye was quiet with no cell or flare noted. The vision was 20/20.", "contents": "Eyelash tolerated in the anterior chamber and cornea. Cilia may be carried into the eye following a penetrating injury. Such a case of a cilium in the anterior chamber is described. The cilium was discovered years later following an injury. A cilium was noted to extend through the cornea into the anterior chamber barely touching the anterior lens capsule. Although the lens surface directly directly adjacent to the foreign body was clear, an anterior capsule lens opacification and area of posterior syncechia were noted at the 3 o'clock meridian. The eye was quiet with no cell or flare noted. The vision was 20/20.", "PMID": 962271}
{"id": "pubmed23n0032_4913", "title": "Experiences with the Bj\u00f6rk-Shiley tilting disc valve in aortic and mitral valve surgery.", "content": "During a four-year period, aortic or mitral valve replacements with the Bj\u00f6rk-Shiley tilting disc valve were performed in 114 cases. The series comprises 75 aortic, 34 mitral and 5 double valve replacements. In the aortic valve group (AVR) concomitant resection of the ascending aorta (cystic medial necrosis aneurysm)) was carried out in 5 cases, aortocoronary bypass in 2 cases and closure of a VSD in one case. Antibiotic prophylaxis and postoperative anticoagulant treatment were used routinely. The hospital mortality rates were6 8.0% in the AVR-group (single aortic valve mortality 7.1 %), 8.8% in the MVR-group and 60% in the double valve group. Late mortality in the AVR-group was 2.8%. There were no late thromboembolic complications in this group. Aortography showed grade II--III regurgitation (paravalvular leaks) in 15 % of the cases. In the MVR-group the late mortality was 9.7%. One patient died because of malfunction (thrombosis) of the prosthesis. The thrombotic complication was obviously caused by discontinuation of anticoagulant therapy. The clinically estimated functional capacity NYHA classification) improved considerably, especially in the MVR-group.", "contents": "Experiences with the Bj\u00f6rk-Shiley tilting disc valve in aortic and mitral valve surgery. During a four-year period, aortic or mitral valve replacements with the Bj\u00f6rk-Shiley tilting disc valve were performed in 114 cases. The series comprises 75 aortic, 34 mitral and 5 double valve replacements. In the aortic valve group (AVR) concomitant resection of the ascending aorta (cystic medial necrosis aneurysm)) was carried out in 5 cases, aortocoronary bypass in 2 cases and closure of a VSD in one case. Antibiotic prophylaxis and postoperative anticoagulant treatment were used routinely. The hospital mortality rates were6 8.0% in the AVR-group (single aortic valve mortality 7.1 %), 8.8% in the MVR-group and 60% in the double valve group. Late mortality in the AVR-group was 2.8%. There were no late thromboembolic complications in this group. Aortography showed grade II--III regurgitation (paravalvular leaks) in 15 % of the cases. In the MVR-group the late mortality was 9.7%. One patient died because of malfunction (thrombosis) of the prosthesis. The thrombotic complication was obviously caused by discontinuation of anticoagulant therapy. The clinically estimated functional capacity NYHA classification) improved considerably, especially in the MVR-group.", "PMID": 962273}
{"id": "pubmed23n0032_4914", "title": "Four years' experience of valve replacement with the Bj\u00f6rk-Shiley tilting-disc prosthesis.", "content": "105 valve replacements with the Bj\u00f6rk-Shiley tilting-disc prosthesis have been performed in 99 patients with an overall mortality of 12%. The hospital mortality for 73 single aortic valve replacements was 10%, for 24 single mitral valve replacements 13%, and for 5 double valve operations 20%. One late death (1%) occurred in a case with single aortic valve replacement. The follow-up period of the first patients with aortic valve replacement is 4 1/2 years, and that of those with mitral valve replacement over 2 1/2 years. The most common late complication in patients with mitral valve replacement was a paravalvular leak (19%), whereas in patients with aortic valve replacement slight haemolytic anaemia (4%) and late thromboembolism (3%) occurred most frequently. 84 of the 87 survivors showed manifest clinical improvement in their preoperative status and increased exercise tolerance.", "contents": "Four years' experience of valve replacement with the Bj\u00f6rk-Shiley tilting-disc prosthesis. 105 valve replacements with the Bj\u00f6rk-Shiley tilting-disc prosthesis have been performed in 99 patients with an overall mortality of 12%. The hospital mortality for 73 single aortic valve replacements was 10%, for 24 single mitral valve replacements 13%, and for 5 double valve operations 20%. One late death (1%) occurred in a case with single aortic valve replacement. The follow-up period of the first patients with aortic valve replacement is 4 1/2 years, and that of those with mitral valve replacement over 2 1/2 years. The most common late complication in patients with mitral valve replacement was a paravalvular leak (19%), whereas in patients with aortic valve replacement slight haemolytic anaemia (4%) and late thromboembolism (3%) occurred most frequently. 84 of the 87 survivors showed manifest clinical improvement in their preoperative status and increased exercise tolerance.", "PMID": 962274}
{"id": "pubmed23n0032_4915", "title": "Reemployment related to functional and haemodynamic changes after aortic or mitral valve replacement.", "content": "The objectives of this longitudinal study were to determine the proportion of individuals who return to work following aortic or mitral valve replacement, and to compare the functional and haemodynamic status in those who return to work with those who do not return to work. The study includes a total of 133 subjects who were all  examined 12--24 months after the operation. Based on this investigation, an overall reemployment rate of 44 % was found. Return to work was more frequent in the younger group than in the older group. Furthermore, while the patient's opinion of the extent of his physical handicap was of some importance when return to work was discussed, there did not appear to be any significant relationship between the working or the non-working group and objective indices of improvement as decrease in heart volume, regression of left ventricular hyperthrophy in the electrocardiogram, increase in cardiac output and decrease in pulmonary wedge pressure. Most important was the observation that the rate of return to work was closely related to the employment status before operations. The conclusion reached was that individuals who return to work were more likely to be those who were employed up to the time of the operation, while return to work after a long period of unemployment before operation was unusual, irrespective of the surgical results.", "contents": "Reemployment related to functional and haemodynamic changes after aortic or mitral valve replacement. The objectives of this longitudinal study were to determine the proportion of individuals who return to work following aortic or mitral valve replacement, and to compare the functional and haemodynamic status in those who return to work with those who do not return to work. The study includes a total of 133 subjects who were all  examined 12--24 months after the operation. Based on this investigation, an overall reemployment rate of 44 % was found. Return to work was more frequent in the younger group than in the older group. Furthermore, while the patient's opinion of the extent of his physical handicap was of some importance when return to work was discussed, there did not appear to be any significant relationship between the working or the non-working group and objective indices of improvement as decrease in heart volume, regression of left ventricular hyperthrophy in the electrocardiogram, increase in cardiac output and decrease in pulmonary wedge pressure. Most important was the observation that the rate of return to work was closely related to the employment status before operations. The conclusion reached was that individuals who return to work were more likely to be those who were employed up to the time of the operation, while return to work after a long period of unemployment before operation was unusual, irrespective of the surgical results.", "PMID": 962275}
{"id": "pubmed23n0032_4916", "title": "Closed mitral valvulotomy.", "content": "During an average follow-up period of five years (from 1966 to 1975) 60 patients, 43 females and 17 males, were subjected to closed mitral valvulotomy at the Oulu University Central Hospital. Two patients died when still in hospital, and one died later at home. 25 % of whole series, and 41 % of the patients with atrial fibrillastion presented with a history of preoperative systemic embolism. Intraoperative embolism occurre in one patient, and late embolism in two patients. All these patients survived. Excellent or good results were recorded for 66 % of the living patients. Significant mitral calcification and/or preoperative regurgitation affected adversely both mortality and functional results. Closed mitral valvulotomy still offers excellent palliation with a minor risk for a significant number of carefully selected patients with mitral stenosis.", "contents": "Closed mitral valvulotomy. During an average follow-up period of five years (from 1966 to 1975) 60 patients, 43 females and 17 males, were subjected to closed mitral valvulotomy at the Oulu University Central Hospital. Two patients died when still in hospital, and one died later at home. 25 % of whole series, and 41 % of the patients with atrial fibrillastion presented with a history of preoperative systemic embolism. Intraoperative embolism occurre in one patient, and late embolism in two patients. All these patients survived. Excellent or good results were recorded for 66 % of the living patients. Significant mitral calcification and/or preoperative regurgitation affected adversely both mortality and functional results. Closed mitral valvulotomy still offers excellent palliation with a minor risk for a significant number of carefully selected patients with mitral stenosis.", "PMID": 962276}
{"id": "pubmed23n0032_4917", "title": "Haemodilution in cardiopulmonary bypass using a gelatine derivative for priming.", "content": "The effects of cardiopulmonary bypass haemodilution and a gelatine derivate for priming were evaluated by serial determinations of the acid-base and electrolyte balances, arterial oxygenation, urinary output, postoperative chest x-ray, cardiac rhythm and electrophoresis in 40 patients undergoing open heart surgery. It was noted that the priming solution used provided an excelllent buffer system to stabilize the derangements in acid-base and electrolyte balance without any side effects in oxygenation, urinary output, cardiac function or plasma proteins. The utilisation of haemodilution in cardiopulmonary bypass is discussed.", "contents": "Haemodilution in cardiopulmonary bypass using a gelatine derivative for priming. The effects of cardiopulmonary bypass haemodilution and a gelatine derivate for priming were evaluated by serial determinations of the acid-base and electrolyte balances, arterial oxygenation, urinary output, postoperative chest x-ray, cardiac rhythm and electrophoresis in 40 patients undergoing open heart surgery. It was noted that the priming solution used provided an excelllent buffer system to stabilize the derangements in acid-base and electrolyte balance without any side effects in oxygenation, urinary output, cardiac function or plasma proteins. The utilisation of haemodilution in cardiopulmonary bypass is discussed.", "PMID": 962277}
{"id": "pubmed23n0032_4918", "title": "Scanning electron microscopic study of the organ of Corti in normal and sound-damaged guinea pigs.", "content": "A method was evolved by which the organ of Corti could be examined in its entirety with the scanning electron microscope, the organ meanwhile retaining its spiral form. This made it possible to assess traumatic effects on the cochlea and qualify lesions in terms of extent, localization and pattern. It was also found possible eventually to cut the same specimen into sections for cellular and subcellular studies. The number of guinea pigs examined totalled 91, divided into three groups. The first group was used to study the anatomy of the organ of Corti with special reference to normal variations and artifacts. Unmistakable indications were found that the longest stereocilia of the inner hair cells are linked to the tectorial membrane. The animals of the second group were exposed to pure tones of high intensity, whereupon, lesions of the organ of Corti were described according to intensity, time, and frequency. Three different types of otologic drills were used to perform mastoidectomies on temporal bones and on the cadaver. The noise produced was analyzed as to intensity and frequency range. It was found that the drill with the lowest rpm (and highest torque) produced the highest noise intensities, at levels which can be traumatic to the human organ of hearing. The animals of the third group were exposed to the amplified noise produced by otologic drills of three different types. The resulting lesions in the organ of Corti were examined by the method described for scanning electron microscopy and compared. In spite of the wide variation in individual lesions, patterns of degeneration of three different types could be distinguished. The high-speed and the very-high-speed drill inflicted less damage on the organ of Corti than the low-speed drill. It is therefore advised to refrain from using the latter drill in prolonged operations.", "contents": "Scanning electron microscopic study of the organ of Corti in normal and sound-damaged guinea pigs. A method was evolved by which the organ of Corti could be examined in its entirety with the scanning electron microscope, the organ meanwhile retaining its spiral form. This made it possible to assess traumatic effects on the cochlea and qualify lesions in terms of extent, localization and pattern. It was also found possible eventually to cut the same specimen into sections for cellular and subcellular studies. The number of guinea pigs examined totalled 91, divided into three groups. The first group was used to study the anatomy of the organ of Corti with special reference to normal variations and artifacts. Unmistakable indications were found that the longest stereocilia of the inner hair cells are linked to the tectorial membrane. The animals of the second group were exposed to pure tones of high intensity, whereupon, lesions of the organ of Corti were described according to intensity, time, and frequency. Three different types of otologic drills were used to perform mastoidectomies on temporal bones and on the cadaver. The noise produced was analyzed as to intensity and frequency range. It was found that the drill with the lowest rpm (and highest torque) produced the highest noise intensities, at levels which can be traumatic to the human organ of hearing. The animals of the third group were exposed to the amplified noise produced by otologic drills of three different types. The resulting lesions in the organ of Corti were examined by the method described for scanning electron microscopy and compared. In spite of the wide variation in individual lesions, patterns of degeneration of three different types could be distinguished. The high-speed and the very-high-speed drill inflicted less damage on the organ of Corti than the low-speed drill. It is therefore advised to refrain from using the latter drill in prolonged operations.", "PMID": 962272}
{"id": "pubmed23n0032_4919", "title": "Rupture and successful repair of the heart following blunt chest injury.", "content": "A case of cardiac rupture secondary to blunt chest injury and its successful surgical repair is described. A review of the literature based on eleven previous similar cases is given.", "contents": "Rupture and successful repair of the heart following blunt chest injury. A case of cardiac rupture secondary to blunt chest injury and its successful surgical repair is described. A review of the literature based on eleven previous similar cases is given.", "PMID": 962278}
{"id": "pubmed23n0032_4920", "title": "Operative treatment of carotid artery occlusion. A follow-up study.", "content": "One hundred consecutive patients have been subjectes to 134 carotid artery reconstructive procedures. 81 patients had TIA syndrome, 2 had acutely progressing stroke and 17 had symptomless stenosis at the carotid artery bifurcation. In 121 instances a bifurcation endarterectomy was performed, in 6 cases the internal or common carotid artery was resected and reconstructed, in 5 cases allograft- and in 2 cases autologous vein was used for the reconstruction. An intraluminal shunt was used in 93 operations. Operative mortality occurred in 4 patients, one was a case of acutely progressing stroke. Late mortality occurred in 16 patients. A follow-up study was carried out in the remaining 80 patients. According to the patients' own judgement symptomatic relief was good in 52.5 %, satisfactory in 40 % and unchanged in 7.5 %.", "contents": "Operative treatment of carotid artery occlusion. A follow-up study. One hundred consecutive patients have been subjectes to 134 carotid artery reconstructive procedures. 81 patients had TIA syndrome, 2 had acutely progressing stroke and 17 had symptomless stenosis at the carotid artery bifurcation. In 121 instances a bifurcation endarterectomy was performed, in 6 cases the internal or common carotid artery was resected and reconstructed, in 5 cases allograft- and in 2 cases autologous vein was used for the reconstruction. An intraluminal shunt was used in 93 operations. Operative mortality occurred in 4 patients, one was a case of acutely progressing stroke. Late mortality occurred in 16 patients. A follow-up study was carried out in the remaining 80 patients. According to the patients' own judgement symptomatic relief was good in 52.5 %, satisfactory in 40 % and unchanged in 7.5 %.", "PMID": 962279}
{"id": "pubmed23n0032_4921", "title": "Acute coeliac artery occlusion: initial collateral blood flow from the superior mesenteric artery. An experimental study.", "content": "The collateral blood flow from the superior mesenteric artery after acute occlusion of the coeliac truck was measured in experiments in dogs. Immediately after clamping of the coeliac axis the collateral flow was about 30 % of the original coeliac blood flow. During an observation time of three hours the collateral flow increased, the corresponding value at the end of experiment being about 50 %. At the same time the blood pressure in the coeliac artery rose from a mean value of 20 mmHg to 35 mmHg. The initial collateral blood flow was evidently not sufficient, since after this short occlusion period ischaemic changes, even necrosis, were observed in the liver and stomach in some of the test animals.", "contents": "Acute coeliac artery occlusion: initial collateral blood flow from the superior mesenteric artery. An experimental study. The collateral blood flow from the superior mesenteric artery after acute occlusion of the coeliac truck was measured in experiments in dogs. Immediately after clamping of the coeliac axis the collateral flow was about 30 % of the original coeliac blood flow. During an observation time of three hours the collateral flow increased, the corresponding value at the end of experiment being about 50 %. At the same time the blood pressure in the coeliac artery rose from a mean value of 20 mmHg to 35 mmHg. The initial collateral blood flow was evidently not sufficient, since after this short occlusion period ischaemic changes, even necrosis, were observed in the liver and stomach in some of the test animals.", "PMID": 962280}
{"id": "pubmed23n0032_4922", "title": "Experience with surgery of the heart valves.", "content": "A review is given of a 15 year experience of valve replacement with Teflon cusps, pericardial cusps, Starr-Edwards, Key-Shiley, Wada-Cutter and Bj\u00f6rk-Shiley artificial valve prosthesis. The Bj\u00f6rk-Shiley tilting disc valves have shown the lowest gradient and blood trauma and excellent durability in more than 1 000 implantations during a 7 year period. Anticoagulation therapy is considered necessary with all types of artificial heart valves. Thrombo-embolism is negligible after aortic valve replacement (1 episode in 4 000 patient months) but still a problem in the mitral position (4 episodes in 1 000 patient months).", "contents": "Experience with surgery of the heart valves. A review is given of a 15 year experience of valve replacement with Teflon cusps, pericardial cusps, Starr-Edwards, Key-Shiley, Wada-Cutter and Bj\u00f6rk-Shiley artificial valve prosthesis. The Bj\u00f6rk-Shiley tilting disc valves have shown the lowest gradient and blood trauma and excellent durability in more than 1 000 implantations during a 7 year period. Anticoagulation therapy is considered necessary with all types of artificial heart valves. Thrombo-embolism is negligible after aortic valve replacement (1 episode in 4 000 patient months) but still a problem in the mitral position (4 episodes in 1 000 patient months).", "PMID": 962282}
{"id": "pubmed23n0032_4923", "title": "The prognostic factors of arterialized bypass veins in the lower extremities.", "content": "A follow up study on 251 in situ bypass procedures on the lower extremities is done 3 years postoperatively. Patency of the bypass is correlated to smoking habits, anticoagulant therapy, age at operation and the diameter of the vein bypass. At follow up 13% of the patients had died and 217 extremities in 195 patients were examined. Using the Chi-square test we found that patency of the bypasses was primarily dependent uon the diameter of the bypass vein, and secondly on smoking habits and anticogulant therapy. Age was only of minor importance for patency.", "contents": "The prognostic factors of arterialized bypass veins in the lower extremities. A follow up study on 251 in situ bypass procedures on the lower extremities is done 3 years postoperatively. Patency of the bypass is correlated to smoking habits, anticoagulant therapy, age at operation and the diameter of the vein bypass. At follow up 13% of the patients had died and 217 extremities in 195 patients were examined. Using the Chi-square test we found that patency of the bypasses was primarily dependent uon the diameter of the bypass vein, and secondly on smoking habits and anticogulant therapy. Age was only of minor importance for patency.", "PMID": 962283}
{"id": "pubmed23n0032_4924", "title": "Surgical treatment of arterial embolism. Fifteen years experience.", "content": "A series of 102 surgical operations for arterial emboli in 85 patients during a fifteen-year period is presented. Seventeen patients were operated upon before the institution of the Fogarty balloon embolectomy catheter. 82 embolectomies using the Fogarty embolectomy catheter were carried out in 68 patients with a mortality of 32%. The amputation rate in the surviving patients was 39%. The most common cause of death was severe underlying heart disease, while the most important reason for amputation after an unsuccessful embolectomy was concomitant occlusive atherosclerosis, and to a lesser degree, a delay in the performance of the embolectomy.", "contents": "Surgical treatment of arterial embolism. Fifteen years experience. A series of 102 surgical operations for arterial emboli in 85 patients during a fifteen-year period is presented. Seventeen patients were operated upon before the institution of the Fogarty balloon embolectomy catheter. 82 embolectomies using the Fogarty embolectomy catheter were carried out in 68 patients with a mortality of 32%. The amputation rate in the surviving patients was 39%. The most common cause of death was severe underlying heart disease, while the most important reason for amputation after an unsuccessful embolectomy was concomitant occlusive atherosclerosis, and to a lesser degree, a delay in the performance of the embolectomy.", "PMID": 962285}
{"id": "pubmed23n0032_4925", "title": "The effect of hepatic dearterialization and re-dearterialization on carcinoid liver metastases.", "content": "A 48-year-old woman with severe carcinoid syndrome caused by multiple liver metastases was treated by hepatic dearterialization. After the operation, urinary 5-HIAA excretion fell transiently to normal levels and the patient was symptom-free for six months. After one year the symptoms of carcinoid syndrome had recurred and a re-dearterialization was performed. A favourable clinical effect was again achieved, but the relief of symptoms lasted only six months. The changes in urinary 5-HIAA excretion and SGOT, SGPT, bilirubin, alkaline phosphatase, cholesterol, PP activity as well as serum protein levels were uniform after both operations to an astonishing degree. Histological specimens taken at reoperation showed that the carcinoid metastases in the anterior parts of the liver were largely necrotic. The necrotic areas has peculiar features, e.g. the necrosis was partly incomplete and ghost like carcinoid structures could be seen.", "contents": "The effect of hepatic dearterialization and re-dearterialization on carcinoid liver metastases. A 48-year-old woman with severe carcinoid syndrome caused by multiple liver metastases was treated by hepatic dearterialization. After the operation, urinary 5-HIAA excretion fell transiently to normal levels and the patient was symptom-free for six months. After one year the symptoms of carcinoid syndrome had recurred and a re-dearterialization was performed. A favourable clinical effect was again achieved, but the relief of symptoms lasted only six months. The changes in urinary 5-HIAA excretion and SGOT, SGPT, bilirubin, alkaline phosphatase, cholesterol, PP activity as well as serum protein levels were uniform after both operations to an astonishing degree. Histological specimens taken at reoperation showed that the carcinoid metastases in the anterior parts of the liver were largely necrotic. The necrotic areas has peculiar features, e.g. the necrosis was partly incomplete and ghost like carcinoid structures could be seen.", "PMID": 962286}
{"id": "pubmed23n0032_4926", "title": "The effect of time factors on blood alcohol levels in accident victims.", "content": "Variations of alcohol involvement (AI) by time as well as the effect of the time interval between the last alcohol intake and accident occurrence, arrival, and specimen collection on blood alcohol concentrations (BAC) were studied in 1012 accident victims. In was found that 53% of accidents on Saturdays were alcohol-related, the corresponding percentage being 39% on Fridays and Sundays and 31% on other days. AI rose from its lowest figure (16%) in the morning to its highest (67%) in the following small and early hours. Most of the alcohol-involved accidents occurred with rising blood alcohol suggesting that this stage of intoxication might have a higher injury risk than the later stage with decreasing blood alcohol. The delay before arrival and specimen collection often led to a drop in BAC values which should be remembered when planning studies on AI in accidents and when interpreting the results of such investigations.", "contents": "The effect of time factors on blood alcohol levels in accident victims. Variations of alcohol involvement (AI) by time as well as the effect of the time interval between the last alcohol intake and accident occurrence, arrival, and specimen collection on blood alcohol concentrations (BAC) were studied in 1012 accident victims. In was found that 53% of accidents on Saturdays were alcohol-related, the corresponding percentage being 39% on Fridays and Sundays and 31% on other days. AI rose from its lowest figure (16%) in the morning to its highest (67%) in the following small and early hours. Most of the alcohol-involved accidents occurred with rising blood alcohol suggesting that this stage of intoxication might have a higher injury risk than the later stage with decreasing blood alcohol. The delay before arrival and specimen collection often led to a drop in BAC values which should be remembered when planning studies on AI in accidents and when interpreting the results of such investigations.", "PMID": 962287}
{"id": "pubmed23n0032_4927", "title": "Intussusception of the appendix caused by an adenovillous papilloma.", "content": "A case of intussusception of the appendix caused by an adenovillous papilloma in the appendix is reported. The condition was combined with acute appendicitis. The different clinical pictures are described. Due to the varied symptoms the diagnosis of intussusception of the appendix is rarely made preoperatively. The aetiology is briefly discussed. The treatment is operative with removal of the appendix or an appropriate resection.", "contents": "Intussusception of the appendix caused by an adenovillous papilloma. A case of intussusception of the appendix caused by an adenovillous papilloma in the appendix is reported. The condition was combined with acute appendicitis. The different clinical pictures are described. Due to the varied symptoms the diagnosis of intussusception of the appendix is rarely made preoperatively. The aetiology is briefly discussed. The treatment is operative with removal of the appendix or an appropriate resection.", "PMID": 962288}
{"id": "pubmed23n0032_4928", "title": "Cardiopulmonary bypass time and renal function.", "content": "Patients undergoing open-heart surgery were divided into two groups according to cardiopulmonary bypass time: in Group I the bypass time was less than 60 minutes (25 patients) and in Group II more than 60 minutes (25 patients). Renal function parameters were investigated preoperatively, during the operation and for two days-postoperatively. The urine volume and excretion of sodium and potassium increased significantly in both groups during the bypass period compared to the values of the pre-bypass period. Significant differences (p less than 0.001) were seen during the bypass period in free water clearance: -0,8 +/- 0.6 ml/min in Group I and -0.2 +/- 0.2 ml/min in Group II, and in creatinine clearance: 114 +/- 42 ml/min in Group I and 60 +/- 12 ml/min in Group II. The inter-group differences in free water and creatinine clearances were significant (p less than 0.001) from the bypass period to the second day period. In Group II, urine osmolality was significantly lower in serum osmolality significantly higher from the bypass period to the third day period compared to the values of Group I. The excretion of sodium and potassium was higher in Group I during bypass and postoperatively and this was reflected in the serum values of sodium and potassium. According to our results, signs of functional renal impairment were seen in the group with the longer perfusion. In this group, however, the patients were older and had a higher preoperative serum creatinine mean value than the patients with the shorter perfusion. The mechanism of functional renal impairment is discussed.", "contents": "Cardiopulmonary bypass time and renal function. Patients undergoing open-heart surgery were divided into two groups according to cardiopulmonary bypass time: in Group I the bypass time was less than 60 minutes (25 patients) and in Group II more than 60 minutes (25 patients). Renal function parameters were investigated preoperatively, during the operation and for two days-postoperatively. The urine volume and excretion of sodium and potassium increased significantly in both groups during the bypass period compared to the values of the pre-bypass period. Significant differences (p less than 0.001) were seen during the bypass period in free water clearance: -0,8 +/- 0.6 ml/min in Group I and -0.2 +/- 0.2 ml/min in Group II, and in creatinine clearance: 114 +/- 42 ml/min in Group I and 60 +/- 12 ml/min in Group II. The inter-group differences in free water and creatinine clearances were significant (p less than 0.001) from the bypass period to the second day period. In Group II, urine osmolality was significantly lower in serum osmolality significantly higher from the bypass period to the third day period compared to the values of Group I. The excretion of sodium and potassium was higher in Group I during bypass and postoperatively and this was reflected in the serum values of sodium and potassium. According to our results, signs of functional renal impairment were seen in the group with the longer perfusion. In this group, however, the patients were older and had a higher preoperative serum creatinine mean value than the patients with the shorter perfusion. The mechanism of functional renal impairment is discussed.", "PMID": 962289}
{"id": "pubmed23n0032_4929", "title": "The effect of nitrous oxide on renal function in open heart surgery.", "content": "The renal function of 20 patients undergoing open-heart surgery was investigated pre-, per- and postoperatively. The patients were anaesthetized with a combination of morphine, halothane, oxygen and nitrous oxide (the nitrous oxide group), and the remaining ten with a combination of morphine, halothane, oxygen and air (the air group). The renal function tests included urine flow, sodium and potassium excretion, and creatinine and free water clearance. As a consequence of the anaesthesia, the mean arterial pressure declined half an hour after induction, being lower than the preoperative level by 15% (p less than 0.001) in the nitrous oxide group and lower by 11% (p less than 0.05) in the air group. At the same time the urine sodium/potassium ratio was 1.3 in the nitrous oxide group and 1.9 in the air group. Free water clearance was significantly (p less than 0.01) greater in the air group. During the perfusion the sodium/potassium excretion ratio was 1.7 in the nitrous oxide group and 4.1 in the air group (p less than 0.01). On the second day, creatinine clearance was significantly lower in the nitrous oxide group than in the air group (p less than 0.05). On the third day, urine flow was significantly higher and urine osmolality significantly lower (p less than 0.05) in the air group than in the nitrous oxide group.", "contents": "The effect of nitrous oxide on renal function in open heart surgery. The renal function of 20 patients undergoing open-heart surgery was investigated pre-, per- and postoperatively. The patients were anaesthetized with a combination of morphine, halothane, oxygen and nitrous oxide (the nitrous oxide group), and the remaining ten with a combination of morphine, halothane, oxygen and air (the air group). The renal function tests included urine flow, sodium and potassium excretion, and creatinine and free water clearance. As a consequence of the anaesthesia, the mean arterial pressure declined half an hour after induction, being lower than the preoperative level by 15% (p less than 0.001) in the nitrous oxide group and lower by 11% (p less than 0.05) in the air group. At the same time the urine sodium/potassium ratio was 1.3 in the nitrous oxide group and 1.9 in the air group. Free water clearance was significantly (p less than 0.01) greater in the air group. During the perfusion the sodium/potassium excretion ratio was 1.7 in the nitrous oxide group and 4.1 in the air group (p less than 0.01). On the second day, creatinine clearance was significantly lower in the nitrous oxide group than in the air group (p less than 0.05). On the third day, urine flow was significantly higher and urine osmolality significantly lower (p less than 0.05) in the air group than in the nitrous oxide group.", "PMID": 962290}
{"id": "pubmed23n0032_4930", "title": "Effect of castration and long-term oral oestrogen therapy with oestriol succinate on serum lipids.", "content": "The effects of continuous oestriol succinate therapy (2 mg/day) of three years' duration on serum cholesterol, triglycerides and phospholipids were investigated on 50 women with a mean age of 48 years. In addition, the effects of castration only were investigated in 10 women with a similar mean age. One month after castration the serum cholesterol, triglyceride and phospholipid levels were significantly raised (p less than 0.05). During oestriol succinate therapy the cholesterol level tended to decrease. In the control group without hormone therapy, the cholesterol level showed a tendency to rise during the three years. The serum triglyceride and phospholipid levels remained almost unchanged during the oestriol succinate therapy and differed not from corresponding values of the control group.", "contents": "Effect of castration and long-term oral oestrogen therapy with oestriol succinate on serum lipids. The effects of continuous oestriol succinate therapy (2 mg/day) of three years' duration on serum cholesterol, triglycerides and phospholipids were investigated on 50 women with a mean age of 48 years. In addition, the effects of castration only were investigated in 10 women with a similar mean age. One month after castration the serum cholesterol, triglyceride and phospholipid levels were significantly raised (p less than 0.05). During oestriol succinate therapy the cholesterol level tended to decrease. In the control group without hormone therapy, the cholesterol level showed a tendency to rise during the three years. The serum triglyceride and phospholipid levels remained almost unchanged during the oestriol succinate therapy and differed not from corresponding values of the control group.", "PMID": 962291}
{"id": "pubmed23n0032_4931", "title": "The microscopical appearance of the subcutaneously transposed spleen.", "content": "The microscopical appearance of rat splenic tissue has been examined after subcutaneous transposition of the spleen and after portal vein ligation in rats subjected to splenic transposition. The histological picture was compared to that of control animals. Apart from congested red pulp and capsular fibrosis in the subcutaneous position did not seem to result in serious histological changes in the spleen. In three patients treated with subcutaneous transposition of resected spleen because of portal hypertension, histological needle biopsies from the splenic tissue were made postoperatively. Principally the same histological changes were found in the clinical as in the experimental material.", "contents": "The microscopical appearance of the subcutaneously transposed spleen. The microscopical appearance of rat splenic tissue has been examined after subcutaneous transposition of the spleen and after portal vein ligation in rats subjected to splenic transposition. The histological picture was compared to that of control animals. Apart from congested red pulp and capsular fibrosis in the subcutaneous position did not seem to result in serious histological changes in the spleen. In three patients treated with subcutaneous transposition of resected spleen because of portal hypertension, histological needle biopsies from the splenic tissue were made postoperatively. Principally the same histological changes were found in the clinical as in the experimental material.", "PMID": 962292}
{"id": "pubmed23n0032_4932", "title": "The host reaction to toxic agents.", "content": "The identification of side effects of chemotherapeutic and environmental agents has assumed a central role in current day medical practice. Since 1962, the formal recognition of the functional role of host factors in drug idiosyncrasies has resulted in the delineation of the discipline of pharmacogenetics. This investigative arena deals with inborn errors of metabolism which affect drug metabolizing enzymes or alter the effects of drugs in the body. In addition to relating to an understanding of the molecular basis of drug toxicity, these considerations are relevant to the teratologic role of alcohol and dilantin and to the role of environmental agents in the pathogenesis of neoplasia.", "contents": "The host reaction to toxic agents. The identification of side effects of chemotherapeutic and environmental agents has assumed a central role in current day medical practice. Since 1962, the formal recognition of the functional role of host factors in drug idiosyncrasies has resulted in the delineation of the discipline of pharmacogenetics. This investigative arena deals with inborn errors of metabolism which affect drug metabolizing enzymes or alter the effects of drugs in the body. In addition to relating to an understanding of the molecular basis of drug toxicity, these considerations are relevant to the teratologic role of alcohol and dilantin and to the role of environmental agents in the pathogenesis of neoplasia.", "PMID": 962293}
{"id": "pubmed23n0032_4933", "title": "Chronic toxic nephropathies--diagnosis and management.", "content": "As a result of industrial and medical progress, man is exposed to an ever changing array of chemicals, drugs and biological products. The kidneys are extremely vulnerable to chronic toxic effects of these substances. Although acute renal failure, nephrotic syndrome and renal tubular disorders result from acute nephrotoxicity, chronic renal failure with renal failure and hypertension result from chronic nephrotoxicity. Heavy metals, analgesic agents and antimicrobials are the common nephrotoxic substance producing chronic renal disease. Medical management consists of preventive exposure measures and early detection of nephrotoxicity by modern industrial medicine. In addition, early clinical diagnosis with appropriate management may prevent the need for chronic hemodialysis and renal transplantation.", "contents": "Chronic toxic nephropathies--diagnosis and management. As a result of industrial and medical progress, man is exposed to an ever changing array of chemicals, drugs and biological products. The kidneys are extremely vulnerable to chronic toxic effects of these substances. Although acute renal failure, nephrotic syndrome and renal tubular disorders result from acute nephrotoxicity, chronic renal failure with renal failure and hypertension result from chronic nephrotoxicity. Heavy metals, analgesic agents and antimicrobials are the common nephrotoxic substance producing chronic renal disease. Medical management consists of preventive exposure measures and early detection of nephrotoxicity by modern industrial medicine. In addition, early clinical diagnosis with appropriate management may prevent the need for chronic hemodialysis and renal transplantation.", "PMID": 962294}
{"id": "pubmed23n0032_4934", "title": "Correlation between dental maturity, height development and sexual maturation in normal girls.", "content": "The correlation between the dental maturity and the different measures of sexual development, i.e. the age at menarche, the ages at breast and pubic hair development, and the age of peak height velocity, was found to be low in healthy girls. Multiple regression analyses between the predictors height and growth rate at the chronological age of a certain stage of the dental development and each of the predictands age at menarche, breast development, and age of peak height velocity improved the residual standard deviation significantly in comparison with the standard deviation of the distribution of these variables.", "contents": "Correlation between dental maturity, height development and sexual maturation in normal girls. The correlation between the dental maturity and the different measures of sexual development, i.e. the age at menarche, the ages at breast and pubic hair development, and the age of peak height velocity, was found to be low in healthy girls. Multiple regression analyses between the predictors height and growth rate at the chronological age of a certain stage of the dental development and each of the predictands age at menarche, breast development, and age of peak height velocity improved the residual standard deviation significantly in comparison with the standard deviation of the distribution of these variables.", "PMID": 962300}
{"id": "pubmed23n0032_4935", "title": "Skeletal maturity of the hand and wrist in Oaxaca school children.", "content": "Skeletal maturity of the hand and wrist (Tanner-Whitehouse II system) was assessed in a sample of 394 school children 5 to 18 years of age, in the city of Oaxaca, Mexico. The socio-economic background of the sample was relatively poor and the group appeared to have a poor nutritional history (via height and weight measurements). At most ages, the mean skeletal ages of Oaxaca school children are below the British means, and about 60 per cent of the children have skeletal ages below their chronological ages.", "contents": "Skeletal maturity of the hand and wrist in Oaxaca school children. Skeletal maturity of the hand and wrist (Tanner-Whitehouse II system) was assessed in a sample of 394 school children 5 to 18 years of age, in the city of Oaxaca, Mexico. The socio-economic background of the sample was relatively poor and the group appeared to have a poor nutritional history (via height and weight measurements). At most ages, the mean skeletal ages of Oaxaca school children are below the British means, and about 60 per cent of the children have skeletal ages below their chronological ages.", "PMID": 962301}
{"id": "pubmed23n0032_4936", "title": "Modes of action of toxic agents.", "content": "The principal modes of action of toxic agents are discussed in relation to the type of chemical bond formed between the poison and the target constituent of tissues. Alteration of enzyme activity, interference with the binding of poisonous chemicals to proteins, intercalation with nucleic acids, disturbances in electrolyte balance and the disorganization of cellular water and membrane lipids are illustrated as toxic processes involving ionic or van der Waals forces. The reactions of heavy metals with tissue nucleophiles and of exogenous nucleophiles with tissue metals are given brief attention in connection with coordinate-covalent binding. Covalent binding of poisons can arise from the incorporation of an antimetabolite into a larger molecule or reactions of electrophiles or free radicals with tissue constituents. These modes of action are illustrated by chemicals that produce necrosis, allergy or cancer.", "contents": "Modes of action of toxic agents. The principal modes of action of toxic agents are discussed in relation to the type of chemical bond formed between the poison and the target constituent of tissues. Alteration of enzyme activity, interference with the binding of poisonous chemicals to proteins, intercalation with nucleic acids, disturbances in electrolyte balance and the disorganization of cellular water and membrane lipids are illustrated as toxic processes involving ionic or van der Waals forces. The reactions of heavy metals with tissue nucleophiles and of exogenous nucleophiles with tissue metals are given brief attention in connection with coordinate-covalent binding. Covalent binding of poisons can arise from the incorporation of an antimetabolite into a larger molecule or reactions of electrophiles or free radicals with tissue constituents. These modes of action are illustrated by chemicals that produce necrosis, allergy or cancer.", "PMID": 962296}
{"id": "pubmed23n0032_4937", "title": "The effects of drugs on embryonic development.", "content": "A review of the literature demonstrated the difficulties in evaluating the teratogenic effect of drugs in man. Since epidemiologic studies provide suggestive rather than definitive data and results of the current drug testing in laboratory animals may not be applicable to man, the need to develop alternative methods of predicting teratogenicity was apparent. To develop such techniques by studying a possible mechanism of teratogenesis, experiments were performed using a teratogenic folic acid-deficiency and antagonism with 9-methyl pteroylglutamic acid in the pregnant rat. Embryos developing abnormally in response ot this regimen consumed oxygen at a significantly decreased rate. Similar significant reductions in oxygen consumption were found both in rat embryos malforming in response to maternal vitamin A acetate administration, and in mouse embryos in response to teratogenic doses of diphenylhydantoin. It was suggested that such measurements of oxidative metabolism or related techniques may have application in predicting drug teratogenicity and could aid present epidemiologic and empiric approaches to identification of human teratogens.", "contents": "The effects of drugs on embryonic development. A review of the literature demonstrated the difficulties in evaluating the teratogenic effect of drugs in man. Since epidemiologic studies provide suggestive rather than definitive data and results of the current drug testing in laboratory animals may not be applicable to man, the need to develop alternative methods of predicting teratogenicity was apparent. To develop such techniques by studying a possible mechanism of teratogenesis, experiments were performed using a teratogenic folic acid-deficiency and antagonism with 9-methyl pteroylglutamic acid in the pregnant rat. Embryos developing abnormally in response ot this regimen consumed oxygen at a significantly decreased rate. Similar significant reductions in oxygen consumption were found both in rat embryos malforming in response to maternal vitamin A acetate administration, and in mouse embryos in response to teratogenic doses of diphenylhydantoin. It was suggested that such measurements of oxidative metabolism or related techniques may have application in predicting drug teratogenicity and could aid present epidemiologic and empiric approaches to identification of human teratogens.", "PMID": 962297}
{"id": "pubmed23n0032_4938", "title": "Monitoring for precursors of health impairment from toxic agents.", "content": "It is essential that one be able to diagnose when disorders are caused by drugs and toxic agents. It is also essential that scientists strive toward the ultimate goal of preventing such disorders. To this end, it is necessary to evaluate laboratory procedures for their capability to detect changes which precede disorders of health impairment. To accomplish this, some knowledge of the relationship between the exposure to a chemical agent and the effect owing to that exposure is required. The complexities of the dose-effect relationship are discussed with regard to the estimation of exposure, the effect owing to the exposure and the population exposed to the chemical agent.", "contents": "Monitoring for precursors of health impairment from toxic agents. It is essential that one be able to diagnose when disorders are caused by drugs and toxic agents. It is also essential that scientists strive toward the ultimate goal of preventing such disorders. To this end, it is necessary to evaluate laboratory procedures for their capability to detect changes which precede disorders of health impairment. To accomplish this, some knowledge of the relationship between the exposure to a chemical agent and the effect owing to that exposure is required. The complexities of the dose-effect relationship are discussed with regard to the estimation of exposure, the effect owing to the exposure and the population exposed to the chemical agent.", "PMID": 962295}
{"id": "pubmed23n0032_4939", "title": "Carriage patterns of Staphylococcus aureus in a healthy non-hospital population of adults and children.", "content": "Bacteriological examination of healthy school children and adults for Staphylococcus aureus by weekly swabbing of nose, throat and hands over extended periods demonstrated a general transition from persistent to transient carrier status between 10 and 20 years of age. Adult transient carriers were commoner among manual than office workers and frequently yielded biochemically atypical isolates, possibly because of acquired resistance to typical strains. Nasal carriage was commonest, but children frequently yielded the organism from both nose and throat.", "contents": "Carriage patterns of Staphylococcus aureus in a healthy non-hospital population of adults and children. Bacteriological examination of healthy school children and adults for Staphylococcus aureus by weekly swabbing of nose, throat and hands over extended periods demonstrated a general transition from persistent to transient carrier status between 10 and 20 years of age. Adult transient carriers were commoner among manual than office workers and frequently yielded biochemically atypical isolates, possibly because of acquired resistance to typical strains. Nasal carriage was commonest, but children frequently yielded the organism from both nose and throat.", "PMID": 962302}
{"id": "pubmed23n0032_4940", "title": "Skeletal maturity and the prediction of age at menarche.", "content": "The regression of age at menarche on the difference between bone age and chronological age was estimated using hand/wrist radiographs taken between the ages of 10 and 15 years of 352 British girls whose ages at menarche were known. The regression was used to determine, in the case of girls who had not yet menstruated at a given chronological age and bone age, (a) the most probable age at menarche, and (b) the age by which 95 per cent would have experienced menarche. The results were compared with predictions made on the basis of chronological age alone. The range of error of predictions based on chronological age only decreases as age increases. This is true also when bone age is taken into account but, at all ages, the error of prediction using bone age as well as chronological age was less than that of prediction based on chronological age alone.", "contents": "Skeletal maturity and the prediction of age at menarche. The regression of age at menarche on the difference between bone age and chronological age was estimated using hand/wrist radiographs taken between the ages of 10 and 15 years of 352 British girls whose ages at menarche were known. The regression was used to determine, in the case of girls who had not yet menstruated at a given chronological age and bone age, (a) the most probable age at menarche, and (b) the age by which 95 per cent would have experienced menarche. The results were compared with predictions made on the basis of chronological age alone. The range of error of predictions based on chronological age only decreases as age increases. This is true also when bone age is taken into account but, at all ages, the error of prediction using bone age as well as chronological age was less than that of prediction based on chronological age alone.", "PMID": 962303}
{"id": "pubmed23n0032_4941", "title": "Mechanism of the toxic action of carbon monoxide.", "content": "Our studies indicate that a high concentration of carboxyhemoglobin (COHb) does not interfere with the O2--carrying capacity of the blood. In dogs, both the transfusion of erythrocytes containing 80 percent COHb and the i.p. injection of carbon monoxide (CO) gas do not produce CO toxicity even though the COHb is above 50 percent. Dogs inhaling CO (13 percent in air) for 15 minutes died within 15 minutes to 65 minutes with an average COHb level of 65 percent. The probable toxic action of CO is on the cellular respiration taking place in the mitochondria when CO competes with O2 for cytochrome a3. The presence of dissolved CO in plasma, which is necessary for CO to enter the tissue, probably occurs when the exchange takes place between alveolar air and the blood in the lungs. When air containing CO is inhaled, there will be a significant CO tension in the blood when it leaves the lungs and when it reaches the organs especially the heart and brain. While COHb level is useful as a clinical measure of CO exposure, the most important mechanism by which CO causes toxicity is its combination with cytochrome oxidase.", "contents": "Mechanism of the toxic action of carbon monoxide. Our studies indicate that a high concentration of carboxyhemoglobin (COHb) does not interfere with the O2--carrying capacity of the blood. In dogs, both the transfusion of erythrocytes containing 80 percent COHb and the i.p. injection of carbon monoxide (CO) gas do not produce CO toxicity even though the COHb is above 50 percent. Dogs inhaling CO (13 percent in air) for 15 minutes died within 15 minutes to 65 minutes with an average COHb level of 65 percent. The probable toxic action of CO is on the cellular respiration taking place in the mitochondria when CO competes with O2 for cytochrome a3. The presence of dissolved CO in plasma, which is necessary for CO to enter the tissue, probably occurs when the exchange takes place between alveolar air and the blood in the lungs. When air containing CO is inhaled, there will be a significant CO tension in the blood when it leaves the lungs and when it reaches the organs especially the heart and brain. While COHb level is useful as a clinical measure of CO exposure, the most important mechanism by which CO causes toxicity is its combination with cytochrome oxidase.", "PMID": 962299}
{"id": "pubmed23n0032_4942", "title": "Validity of the ultrasonic technique as a method of measuring subcutaneous adipose tissue.", "content": "Subcutaneous adipose tissue measurements, at abdomen, suprailiac, subscapular, and mid-triceps sites, made with the ultrasonic and skinfold caliper techniques were compared using data from 20 women and 17 men from a pool of subjects of 22 women and 20 men. In addition, comparisons were made between ultrasonic, soft-tissue roentgenographic and skinfold caliper techniques at the suprailiac and mid-triceps sites on 6 women and 12 men. Repeat measurements with the ultrasonic technique produced correlation coefficients of 0-87 and above. Correlations between skinfold caliper and ultrasonic measurements were generally higher among women than men over each of the four sites. A highly significant relationship, r = 0-88, was observed between the ultrasonic measurements and soft-tissue roentgenograms over the mid-triceps site. The correlation between the fat-muscle interface measured on the roentgenogram and ultrasonic measurements at the suprailiac site was 0-78. Multiple echoes and interfaces were observed at the suprailiac site on some subjects.", "contents": "Validity of the ultrasonic technique as a method of measuring subcutaneous adipose tissue. Subcutaneous adipose tissue measurements, at abdomen, suprailiac, subscapular, and mid-triceps sites, made with the ultrasonic and skinfold caliper techniques were compared using data from 20 women and 17 men from a pool of subjects of 22 women and 20 men. In addition, comparisons were made between ultrasonic, soft-tissue roentgenographic and skinfold caliper techniques at the suprailiac and mid-triceps sites on 6 women and 12 men. Repeat measurements with the ultrasonic technique produced correlation coefficients of 0-87 and above. Correlations between skinfold caliper and ultrasonic measurements were generally higher among women than men over each of the four sites. A highly significant relationship, r = 0-88, was observed between the ultrasonic measurements and soft-tissue roentgenograms over the mid-triceps site. The correlation between the fat-muscle interface measured on the roentgenogram and ultrasonic measurements at the suprailiac site was 0-78. Multiple echoes and interfaces were observed at the suprailiac site on some subjects.", "PMID": 962304}
{"id": "pubmed23n0032_4943", "title": "Pitfalls in iodine-125 digoxin measurement.", "content": "Pitfalls in Iodine-125 digoxin methods are reviewed. Problems may arise from instrumentation, reagents, methodology, medications and variation in patient specimens. The laboratory has an obligation to avoid these errors and ways are available to accomplish this.", "contents": "Pitfalls in iodine-125 digoxin measurement. Pitfalls in Iodine-125 digoxin methods are reviewed. Problems may arise from instrumentation, reagents, methodology, medications and variation in patient specimens. The laboratory has an obligation to avoid these errors and ways are available to accomplish this.", "PMID": 962298}
{"id": "pubmed23n0032_4944", "title": "The age of menarche in Nigerian girls.", "content": "The median ages at menarche were obtained from a sample of 2029 urban and 328 rural Nigerian girls by the status quo method, and calculated by probits. The median age at menarche for the urban girls was 13-70 +/- 0-03 years whilst it was 14-50 +/- 0-09 years for the rural girls. There were significant differences in the median menarcheal ages of the girls according to the level of education and the occupation of the parents, and their geographical residence. The number of siblings and the birth rank of the girls did not influence the age of onset of menstruation. A secular trend towards earlier menarcheal age at a rate of 5 to 6 months per decade was observed amongst children of the professionally privileged group. The earliest menarche occurred amongst girls with university-educated fathers or mothers; in this group the median was 13-26 +/- 0-06 years, a value comparable with current European medians.", "contents": "The age of menarche in Nigerian girls. The median ages at menarche were obtained from a sample of 2029 urban and 328 rural Nigerian girls by the status quo method, and calculated by probits. The median age at menarche for the urban girls was 13-70 +/- 0-03 years whilst it was 14-50 +/- 0-09 years for the rural girls. There were significant differences in the median menarcheal ages of the girls according to the level of education and the occupation of the parents, and their geographical residence. The number of siblings and the birth rank of the girls did not influence the age of onset of menstruation. A secular trend towards earlier menarcheal age at a rate of 5 to 6 months per decade was observed amongst children of the professionally privileged group. The earliest menarche occurred amongst girls with university-educated fathers or mothers; in this group the median was 13-26 +/- 0-06 years, a value comparable with current European medians.", "PMID": 962305}
{"id": "pubmed23n0032_4945", "title": "Weight and skinfold variation at menarche and the critical body weight hypothesis.", "content": "The weight and skinfolds of 36 longitudinally studied British girls were analysed to determine their variability from two years pre-menarche to two years post-menarche. If the critical body weight hypothesis of Frisch and Revelle (1970) is true, a reduction in the variability of these parameters would be expected at menarche compared to times before and after this event. No such reductions in variability were apparent.", "contents": "Weight and skinfold variation at menarche and the critical body weight hypothesis. The weight and skinfolds of 36 longitudinally studied British girls were analysed to determine their variability from two years pre-menarche to two years post-menarche. If the critical body weight hypothesis of Frisch and Revelle (1970) is true, a reduction in the variability of these parameters would be expected at menarche compared to times before and after this event. No such reductions in variability were apparent.", "PMID": 962306}
{"id": "pubmed23n0032_4946", "title": "The secular trend in age at menarche in the Netherlands.", "content": "The mean age at menarche of girls in Utrecht city, the Netherlands, in 1971 and 1972 was estimated by probit analysis as 13-37 years with a standard deviation of 1-17 years. This result is compared with similar data obtained in the Netherlands in 1955 and 1965.", "contents": "The secular trend in age at menarche in the Netherlands. The mean age at menarche of girls in Utrecht city, the Netherlands, in 1971 and 1972 was estimated by probit analysis as 13-37 years with a standard deviation of 1-17 years. This result is compared with similar data obtained in the Netherlands in 1955 and 1965.", "PMID": 962307}
{"id": "pubmed23n0032_4947", "title": "Antenatal events and postnatal growth and survival of children in a rural Guatemalan village.", "content": "The biological features of all pregnant women and newborn infants in a typical rural village of Guatemala have been studied form 1964 to the present; 458 pregnancied have been analysed. The mothers averaged 143.1 cm in height 52.9 kg in weight and 9 mm in triceps skinfold. The diet of most was adequate in percentage protein but inadequate in iron and other nutrients. Infection was common, two or more episodes occurring in 40 per cent of pregnancies. The newborn population had a birthweight averaging 2533 g and length 45.6 cm. Thirty-four per cent were less than 2501 g at 37 weeks gestation or over; seven per cent were pre-term. The infants' growth was followed till age six years. Survival correlated strongly with birth weight and gestational age. Pre-term infants showed the poorest survival in neonatal and postneonatal infancy; but the survivors thrived therafter. The term-small-for-gestational age infants had poor survival in infancy and during the second and third years. Term infants with adequate weight-for-gestational age had the best survival rate. Postnatal physical growth correlated with birth weight and gestational age but pre-term adequate-for-gestational age infants showed a rate of growth as good as that of term infants.", "contents": "Antenatal events and postnatal growth and survival of children in a rural Guatemalan village. The biological features of all pregnant women and newborn infants in a typical rural village of Guatemala have been studied form 1964 to the present; 458 pregnancied have been analysed. The mothers averaged 143.1 cm in height 52.9 kg in weight and 9 mm in triceps skinfold. The diet of most was adequate in percentage protein but inadequate in iron and other nutrients. Infection was common, two or more episodes occurring in 40 per cent of pregnancies. The newborn population had a birthweight averaging 2533 g and length 45.6 cm. Thirty-four per cent were less than 2501 g at 37 weeks gestation or over; seven per cent were pre-term. The infants' growth was followed till age six years. Survival correlated strongly with birth weight and gestational age. Pre-term infants showed the poorest survival in neonatal and postneonatal infancy; but the survivors thrived therafter. The term-small-for-gestational age infants had poor survival in infancy and during the second and third years. Term infants with adequate weight-for-gestational age had the best survival rate. Postnatal physical growth correlated with birth weight and gestational age but pre-term adequate-for-gestational age infants showed a rate of growth as good as that of term infants.", "PMID": 962308}
{"id": "pubmed23n0032_4948", "title": "Handedness and the cerebral representation of speech.", "content": "Hand preferences vary not discretely but continuously and the continuum of preference is closely and reliably linked with a continuous roughly Normal distribution of differences between the hands in skill. Questions about handedness can be usefully formulated in terms of the distribution of differences in skill and in terms of the theshold or criterion which divided those likely to be classified as right handers from those likely to be classified as left handers. A theory of the origin of hand preferences suggests that three factors are involved. The first in non-genetic accidental variation in the development of the two sides of the body in all bilaterally symmetrical species showing limb preferences. The second in a systematic bias to the right hand in man, probably linked with the tendency for the left hemisphere to serve speech. The third is socio-cultural factors affecting the expression of sinistrality. An explanation of the relation between hand preference and cerebral speech laterality requires the assumption that in some individuals the factor biasing speech to the left hemisphere is absent. This would imply that the bias is not a universal species character but that it is subject to genetic variation. Making the simplest possible further assumptions that in the absence of bias, the laterality of handedness and of cerebral speech are independent and each governed by chance, it is possible to account for the distribution of right and left handers with dysphasias following right and left unilateral cerebral lesions.", "contents": "Handedness and the cerebral representation of speech. Hand preferences vary not discretely but continuously and the continuum of preference is closely and reliably linked with a continuous roughly Normal distribution of differences between the hands in skill. Questions about handedness can be usefully formulated in terms of the distribution of differences in skill and in terms of the theshold or criterion which divided those likely to be classified as right handers from those likely to be classified as left handers. A theory of the origin of hand preferences suggests that three factors are involved. The first in non-genetic accidental variation in the development of the two sides of the body in all bilaterally symmetrical species showing limb preferences. The second in a systematic bias to the right hand in man, probably linked with the tendency for the left hemisphere to serve speech. The third is socio-cultural factors affecting the expression of sinistrality. An explanation of the relation between hand preference and cerebral speech laterality requires the assumption that in some individuals the factor biasing speech to the left hemisphere is absent. This would imply that the bias is not a universal species character but that it is subject to genetic variation. Making the simplest possible further assumptions that in the absence of bias, the laterality of handedness and of cerebral speech are independent and each governed by chance, it is possible to account for the distribution of right and left handers with dysphasias following right and left unilateral cerebral lesions.", "PMID": 962309}
{"id": "pubmed23n0032_4949", "title": "Developmental correlates of handedness in a national sample of 11-year-olds.", "content": "Some developmental correlates of hand preference in a large nationally representative sample of 11-year-old schoolchildren were examined. Non-right handedness was found to have slight, but statistically significant, associations with deficits of performance on certain standardized attainment and ability tests; with \"poor speech\" as assessed impressionistically by teachers in school; and with \"poor control of hands\" as similarly assessed by teachers. Non-right handedness was not associated with defects of articulation or stammering as assessed by doctors in a medical examination; nor withperformance on a clinical speech test; nor with writing productivity or syntactic maturity. There were substantial sex differences in the frequencies of non-right handedness, but no social class differences.", "contents": "Developmental correlates of handedness in a national sample of 11-year-olds. Some developmental correlates of hand preference in a large nationally representative sample of 11-year-old schoolchildren were examined. Non-right handedness was found to have slight, but statistically significant, associations with deficits of performance on certain standardized attainment and ability tests; with \"poor speech\" as assessed impressionistically by teachers in school; and with \"poor control of hands\" as similarly assessed by teachers. Non-right handedness was not associated with defects of articulation or stammering as assessed by doctors in a medical examination; nor withperformance on a clinical speech test; nor with writing productivity or syntactic maturity. There were substantial sex differences in the frequencies of non-right handedness, but no social class differences.", "PMID": 962310}
{"id": "pubmed23n0032_4950", "title": "The rare allele SOD-A(2) in the Italian population.", "content": "Data are reported on the distribution of SOD-A electrophoretic phenotypes in the Italian population, and on the concentration of SODA-A-2-1 phenotype in a family group of a small village. The frequency of the rate allele SOD-A(2) is estimated and compared with the frequency in other populations. By applying a model of population dynamics for rare neutral genes estimates are derived of the mean first arrival time and mean age, in generations, of SOD-A(2) allele.", "contents": "The rare allele SOD-A(2) in the Italian population. Data are reported on the distribution of SOD-A electrophoretic phenotypes in the Italian population, and on the concentration of SODA-A-2-1 phenotype in a family group of a small village. The frequency of the rate allele SOD-A(2) is estimated and compared with the frequency in other populations. By applying a model of population dynamics for rare neutral genes estimates are derived of the mean first arrival time and mean age, in generations, of SOD-A(2) allele.", "PMID": 962311}
{"id": "pubmed23n0032_4951", "title": "Unifactorially inherited attributes of the population of Holy Island, Northumberland.", "content": "The genetic polymorphisms of the Holy Island population show some differences from a mainland series. The significance of the results is discussed in terms of specialized gene flow and population size. Some interactions between the polymorphic systems are discussed.", "contents": "Unifactorially inherited attributes of the population of Holy Island, Northumberland. The genetic polymorphisms of the Holy Island population show some differences from a mainland series. The significance of the results is discussed in terms of specialized gene flow and population size. Some interactions between the polymorphic systems are discussed.", "PMID": 962312}
{"id": "pubmed23n0032_4952", "title": "A factor analysis of Ethiopian children's measurements.", "content": "Thirteen anthropometric measurements of Kaffa district schoolchildren in Ethiopia were factor analysed. Two factors emerged which may be interpreted as (a) body size and (b) fat mass.", "contents": "A factor analysis of Ethiopian children's measurements. Thirteen anthropometric measurements of Kaffa district schoolchildren in Ethiopia were factor analysed. Two factors emerged which may be interpreted as (a) body size and (b) fat mass.", "PMID": 962313}
{"id": "pubmed23n0032_4953", "title": "Age of onset of puberty in urban Argentinian children.", "content": "A cross-sectional study in La Plata city, Argentina yielded mean ages of onset of breast and pubic hair developments in 504 girls and of genitalia development in 498 boys of 10.8, 11.0 and 11.8 years respectively. These values are close to those reported previously for various other populations.", "contents": "Age of onset of puberty in urban Argentinian children. A cross-sectional study in La Plata city, Argentina yielded mean ages of onset of breast and pubic hair developments in 504 girls and of genitalia development in 498 boys of 10.8, 11.0 and 11.8 years respectively. These values are close to those reported previously for various other populations.", "PMID": 962314}
{"id": "pubmed23n0032_4954", "title": "Thoracic and lumbar spinal curvatures in Nigerian adults.", "content": "The thoracic and lumbar curvatures of 105 Nigerian adults have been measured with a flexible rule (flexicurve). The lumbar curvature is relatively greater in women. Compared with a previous survey of Europeans, the curvatures in both men and women appear to be about 20 per cent greater in Nigerians. This is likely to be a genetic difference but the Nigerian practice of carrying heavy loads on the head may be a contributory factor.", "contents": "Thoracic and lumbar spinal curvatures in Nigerian adults. The thoracic and lumbar curvatures of 105 Nigerian adults have been measured with a flexible rule (flexicurve). The lumbar curvature is relatively greater in women. Compared with a previous survey of Europeans, the curvatures in both men and women appear to be about 20 per cent greater in Nigerians. This is likely to be a genetic difference but the Nigerian practice of carrying heavy loads on the head may be a contributory factor.", "PMID": 962315}
{"id": "pubmed23n0032_4955", "title": "The genetics of specific reading disability.", "content": "Members of the immediate families of twenty children with specific reading disability were examined to determine the prevalence of reading disability within the families. A procedure was developed for identifying adults who may have compensated for a disability manifested more clearly in childhood. Forty-five percent of 75 first-degree relatives of the parents were affected and there was a significantly greater number of affected male relatives than females. No single mode of genetic transmission is evident after inspection of the pedigrees. It is suggested that the disorder is genetically heterogeneous and that subgroups of disabled readers should be looked for.", "contents": "The genetics of specific reading disability. Members of the immediate families of twenty children with specific reading disability were examined to determine the prevalence of reading disability within the families. A procedure was developed for identifying adults who may have compensated for a disability manifested more clearly in childhood. Forty-five percent of 75 first-degree relatives of the parents were affected and there was a significantly greater number of affected male relatives than females. No single mode of genetic transmission is evident after inspection of the pedigrees. It is suggested that the disorder is genetically heterogeneous and that subgroups of disabled readers should be looked for.", "PMID": 962317}
{"id": "pubmed23n0032_4956", "title": "The segregation of human chromosome polymorphisms.", "content": "The inheritance of C-band and Q-band polymorphisms have been investigated in 32 families. C-band inversion and size polymorphisms appear to segregate in a Mendelian manner, with the possible exception of the very large variant of chromosome number 9 which may be selectively inherited. There is some evidence to suggest a preferential segregation of Brilliant Q-band polymorphisms but it may be that this is an artifact caused by insufficient data and scoring error.", "contents": "The segregation of human chromosome polymorphisms. The inheritance of C-band and Q-band polymorphisms have been investigated in 32 families. C-band inversion and size polymorphisms appear to segregate in a Mendelian manner, with the possible exception of the very large variant of chromosome number 9 which may be selectively inherited. There is some evidence to suggest a preferential segregation of Brilliant Q-band polymorphisms but it may be that this is an artifact caused by insufficient data and scoring error.", "PMID": 962318}
{"id": "pubmed23n0032_4957", "title": "Optimal and discriminating birth weights in human populations.", "content": "A new model for estimating the optimal birth weight of a sample of infants from a human population, based on conditional survival probability, is developed. Also, a method is developed for estimating the 'lower discriminating birth weight' (LDBW), the weight below which an infant has a greater probability of dying than surviving, and the 'upper discriminating birth weight' (UDBW), the weight above which an infant has a greater probability of dying than surviving. These models are tested using previously published human birth weight data from British, German, Indian, Italian, and United States samples. The relationship between LDBW and 'prematurity' is briefly discussed.", "contents": "Optimal and discriminating birth weights in human populations. A new model for estimating the optimal birth weight of a sample of infants from a human population, based on conditional survival probability, is developed. Also, a method is developed for estimating the 'lower discriminating birth weight' (LDBW), the weight below which an infant has a greater probability of dying than surviving, and the 'upper discriminating birth weight' (UDBW), the weight above which an infant has a greater probability of dying than surviving. These models are tested using previously published human birth weight data from British, German, Indian, Italian, and United States samples. The relationship between LDBW and 'prematurity' is briefly discussed.", "PMID": 962319}
{"id": "pubmed23n0032_4958", "title": "Differential growth of human foetal gonads with respect to sex and body side.", "content": "Measurements have been carried out on the gonads of 54 human foetuses, 29 males and 25 females. Crown-rump lengths ranged between 140 and 212 mm. The criteria used were fresh weight, total protein contents and total DNA contents. It was found that for any given crown-rump length class, the mean values of testes exceeded those of ovaries for all three criteria. It was also found that the mean values of right gonads exceeded those of left gonads. Since in true hermaphrodites, testes are preferentially situated on the right and ovaries on the left side, it is concluded that there is a causal relationship between increased growth rate and testicular differentiation. It is suggested that in normal development this function is performed by the Y chromosome.", "contents": "Differential growth of human foetal gonads with respect to sex and body side. Measurements have been carried out on the gonads of 54 human foetuses, 29 males and 25 females. Crown-rump lengths ranged between 140 and 212 mm. The criteria used were fresh weight, total protein contents and total DNA contents. It was found that for any given crown-rump length class, the mean values of testes exceeded those of ovaries for all three criteria. It was also found that the mean values of right gonads exceeded those of left gonads. Since in true hermaphrodites, testes are preferentially situated on the right and ovaries on the left side, it is concluded that there is a causal relationship between increased growth rate and testicular differentiation. It is suggested that in normal development this function is performed by the Y chromosome.", "PMID": 962320}
{"id": "pubmed23n0032_4959", "title": "A matrix method for calculating recurrence risks of unilocal disorders for genetic counselling.", "content": "A matrix method to calculate conditional likelihoods in a pedigree and use them to determine recurrence risks for unilocal disorders in genetic risks is presented in this paper. Different matrices are assigned to individual members of the pedigree and combined into matrix expressions. The method is explained for X-linked recessive conditions and autosomal dominant conditions with incomplete penetrance.", "contents": "A matrix method for calculating recurrence risks of unilocal disorders for genetic counselling. A matrix method to calculate conditional likelihoods in a pedigree and use them to determine recurrence risks for unilocal disorders in genetic risks is presented in this paper. Different matrices are assigned to individual members of the pedigree and combined into matrix expressions. The method is explained for X-linked recessive conditions and autosomal dominant conditions with incomplete penetrance.", "PMID": 962322}
{"id": "pubmed23n0032_4960", "title": "The use of matrices in calculating mendelian probabilities.", "content": "It is shown that the method of calculation of probabilities for genetic counselling developed by Bolling, Chase and Murphy can be generalized to give the relative probabilities for any single individual in any pedigree for any mendelian condition. The calculations only require matrix multiplications (of one kind or another), and the expressions can be written down and evaluated directly, whether by hand or by computer.", "contents": "The use of matrices in calculating mendelian probabilities. It is shown that the method of calculation of probabilities for genetic counselling developed by Bolling, Chase and Murphy can be generalized to give the relative probabilities for any single individual in any pedigree for any mendelian condition. The calculations only require matrix multiplications (of one kind or another), and the expressions can be written down and evaluated directly, whether by hand or by computer.", "PMID": 962323}
{"id": "pubmed23n0032_4961", "title": "Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderma pigmentosum.", "content": "The frequency of BrdU-induced sister chromatid exchanges (SCE) in cultured lymphocytes from patients with ataxia telangiectasia, Werner syndrome, and xeroderma pigmentosum was normal. The rate was increased in xeroderma pigmentosum following exposure to ultraviolet light and spontaneously raised in the Bloom syndrome. Quadriradial exchanges between homologous chromosomes in Bloom syndrome not only involve sister chromatids but also homologous (non-sister) chromatids. This could result in the formation of recombinant chromosomes and is viewed as a genetically determined form of increased somatic recombination in man. Endoreduplicated metaphases showed 'twin' and 'single' exchanges in a 1:2 ratio. This suggests a comparable frequency of exchanges at both divisions and provides evidence for the polarity of the chromatid subunits and the presence of a single chain of DNA.", "contents": "Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderma pigmentosum. The frequency of BrdU-induced sister chromatid exchanges (SCE) in cultured lymphocytes from patients with ataxia telangiectasia, Werner syndrome, and xeroderma pigmentosum was normal. The rate was increased in xeroderma pigmentosum following exposure to ultraviolet light and spontaneously raised in the Bloom syndrome. Quadriradial exchanges between homologous chromosomes in Bloom syndrome not only involve sister chromatids but also homologous (non-sister) chromatids. This could result in the formation of recombinant chromosomes and is viewed as a genetically determined form of increased somatic recombination in man. Endoreduplicated metaphases showed 'twin' and 'single' exchanges in a 1:2 ratio. This suggests a comparable frequency of exchanges at both divisions and provides evidence for the polarity of the chromatid subunits and the presence of a single chain of DNA.", "PMID": 962324}
{"id": "pubmed23n0032_4962", "title": "Visual classification of banded human chromosomes. I. Karyotyping compared with classification of isolated chromosomes.", "content": "Visual karyotyping and visual classification of isolated chromosomes was carried out by seven investigators on 22 trypsin banded metaphases of average quality. The karyotyping experiment resulted in an average error rate of 0-1% (zero-0-4%) and the classification of isolated chromosomes resulted in an error rate of 3% (2-5%). The B and F group chromosomes were found to be most difficult to classify when isolated, while no errors were made of the no. 1 and the X chromosome. Large differences were seen in the resulting error pattern for the individual investigators both with regard to their total error rate and also the chromosome types which they most frequently misclassified. Based upon these error patterns it is suggested that more than 95% of the chromosomes in an average quality material contain features upon which a reliable visual classification can be made. Thus there may be a potential possibility that these chromosomes may be classified by computer on the basis of these features. The fact that visual karyotyping is much more reliable than visual classification of isolated chromosomes indicated that computer classification of chromosomes should include programming capable of making appropriate comparison between the chromosomes in the metaphase and at the same time take into account the expected presence of 23 chromosome pairs for normal cells. This would simulate the human performance of visual karyotyping and make a classification possible of at least some of the remaining 5% difficult chromosomes.", "contents": "Visual classification of banded human chromosomes. I. Karyotyping compared with classification of isolated chromosomes. Visual karyotyping and visual classification of isolated chromosomes was carried out by seven investigators on 22 trypsin banded metaphases of average quality. The karyotyping experiment resulted in an average error rate of 0-1% (zero-0-4%) and the classification of isolated chromosomes resulted in an error rate of 3% (2-5%). The B and F group chromosomes were found to be most difficult to classify when isolated, while no errors were made of the no. 1 and the X chromosome. Large differences were seen in the resulting error pattern for the individual investigators both with regard to their total error rate and also the chromosome types which they most frequently misclassified. Based upon these error patterns it is suggested that more than 95% of the chromosomes in an average quality material contain features upon which a reliable visual classification can be made. Thus there may be a potential possibility that these chromosomes may be classified by computer on the basis of these features. The fact that visual karyotyping is much more reliable than visual classification of isolated chromosomes indicated that computer classification of chromosomes should include programming capable of making appropriate comparison between the chromosomes in the metaphase and at the same time take into account the expected presence of 23 chromosome pairs for normal cells. This would simulate the human performance of visual karyotyping and make a classification possible of at least some of the remaining 5% difficult chromosomes.", "PMID": 962325}
{"id": "pubmed23n0032_4963", "title": "C- and Q-band polymorphisms in the chromosomes of three human populations.", "content": "The incidence of the various classes of C-band and Q-band polymorphisms on ten pairs of chromosomes in the human complement have been investigated in three Scottish populations, two from the mainland (newborn and 14 year olds) and one, of individuals over 65 years, from an island in the Outer Hebrides. Although there is an overall similarity between the populations, there are some differences, especially with the island group. For all populations, over 90% of the C-band size variants fall within the medium class. Rather more variation is found in the Q-band intensity polymorphisms: the island population appears to have fewer Brilliant and Intense variants than do the other two groups, 2-9 per person as compared to 4-2 and 3-9 for the newborn and 14 year olds respectively; this may be an age difference rather than a population difference.", "contents": "C- and Q-band polymorphisms in the chromosomes of three human populations. The incidence of the various classes of C-band and Q-band polymorphisms on ten pairs of chromosomes in the human complement have been investigated in three Scottish populations, two from the mainland (newborn and 14 year olds) and one, of individuals over 65 years, from an island in the Outer Hebrides. Although there is an overall similarity between the populations, there are some differences, especially with the island group. For all populations, over 90% of the C-band size variants fall within the medium class. Rather more variation is found in the Q-band intensity polymorphisms: the island population appears to have fewer Brilliant and Intense variants than do the other two groups, 2-9 per person as compared to 4-2 and 3-9 for the newborn and 14 year olds respectively; this may be an age difference rather than a population difference.", "PMID": 962326}
{"id": "pubmed23n0032_4964", "title": "Expression of a mouse immunoglobulin V gene in homozygote and heterozygotes.", "content": "Our earlier work had demonstrated a fine-specificity idiotype in anti-NP antibodies of C57Bl/6 mice. It was controlled by a VH gene (VH-NPb). The idiotype could be demonstrated in all anti-NP of the C50Bl/6 mice (IgG, IgA, IgM including natural anti-NP). The VH-NPb gene is condominant with CBA allele F1 hybrid mice in the production of natural antibodies but dominant over the CBA allele in immune antibody formation (92% of F1 mice were like C57Bl/6). Probably the product of this gene has a higher affinity for NP than the average affinity of idiotype-negative antibody in the F1 hybrids. Both the codominance in natural antibodies and the dominance in immune antibodies had the peculiar characteristic that genotypically identical individuals varied a great deal. The share of idiotype-positive antibody in the total natural anti-NP titre of (C57Bl/6 x CBA)F1 mice varied from less than 33% to more than 67%. In immune antibodies individual differences were still greater, in ca 92% of mice only idiotype-positive anti-NP could be detected and in ca 8% only idiotype-negative antibody was detectable. Only few F1 hybrid mice had detectable amounts of both types. Homozygous mice of the LP strain behaved in all aspects like the F1 mice. Of their natural anti-NP titres 50% were due to idiotype-positive antibody and in the immune antibodies the figure was 86%. Individual non-immune sera again contained varying mixtures of idiotype-positive and idiotype-negative antibody, but immune sera contained only one or the other type. The third allotype b strain tested, C57Bl/Ka, took an intermediary position between LP and C57Bl/6. Ca 70% of natural anti-NP titres were due to idiotype-positive antibody but all immune anti-NP was idiotype-positive.", "contents": "Expression of a mouse immunoglobulin V gene in homozygote and heterozygotes. Our earlier work had demonstrated a fine-specificity idiotype in anti-NP antibodies of C57Bl/6 mice. It was controlled by a VH gene (VH-NPb). The idiotype could be demonstrated in all anti-NP of the C50Bl/6 mice (IgG, IgA, IgM including natural anti-NP). The VH-NPb gene is condominant with CBA allele F1 hybrid mice in the production of natural antibodies but dominant over the CBA allele in immune antibody formation (92% of F1 mice were like C57Bl/6). Probably the product of this gene has a higher affinity for NP than the average affinity of idiotype-negative antibody in the F1 hybrids. Both the codominance in natural antibodies and the dominance in immune antibodies had the peculiar characteristic that genotypically identical individuals varied a great deal. The share of idiotype-positive antibody in the total natural anti-NP titre of (C57Bl/6 x CBA)F1 mice varied from less than 33% to more than 67%. In immune antibodies individual differences were still greater, in ca 92% of mice only idiotype-positive anti-NP could be detected and in ca 8% only idiotype-negative antibody was detectable. Only few F1 hybrid mice had detectable amounts of both types. Homozygous mice of the LP strain behaved in all aspects like the F1 mice. Of their natural anti-NP titres 50% were due to idiotype-positive antibody and in the immune antibodies the figure was 86%. Individual non-immune sera again contained varying mixtures of idiotype-positive and idiotype-negative antibody, but immune sera contained only one or the other type. The third allotype b strain tested, C57Bl/Ka, took an intermediary position between LP and C57Bl/6. Ca 70% of natural anti-NP titres were due to idiotype-positive antibody but all immune anti-NP was idiotype-positive.", "PMID": 962327}
{"id": "pubmed23n0032_4965", "title": "Immunodeficiencies in severe atopic dermatitis. Depressed chemotaxis and lymphocyte transformation.", "content": "Various reports have indicated assorted immune defects in atopic dermatitis, but the prevalence and degree of the defects remain unclear. We assessed various immunological factors in 14 patients with atopic dermatitis to determine whether immunodeficiencies were present consistently and were reflected by the patients' clinical characteristics. A high incidence of cutaneous infection was noted. Cutaneous delayed-hypersensitivity testing showed anergy in eight (67%) patients. Only the seven patients with the most severe condition showed altered leukocyte function, as determined by polymorphonuclear and mononuclear leukocyte chemotaxis and by lymphocyte responsiveness to phytohemaglutinin. All three cell types where shown to be simultaneously dysfunctional during  severe atopic flares. Chemotactic studies during clinical remissions disclosed notable improvement in cell migration. Serum IgE levels were elevated in each patient, but did not correlate with the degree of cutaneous anergy or altered leukocyte function.", "contents": "Immunodeficiencies in severe atopic dermatitis. Depressed chemotaxis and lymphocyte transformation. Various reports have indicated assorted immune defects in atopic dermatitis, but the prevalence and degree of the defects remain unclear. We assessed various immunological factors in 14 patients with atopic dermatitis to determine whether immunodeficiencies were present consistently and were reflected by the patients' clinical characteristics. A high incidence of cutaneous infection was noted. Cutaneous delayed-hypersensitivity testing showed anergy in eight (67%) patients. Only the seven patients with the most severe condition showed altered leukocyte function, as determined by polymorphonuclear and mononuclear leukocyte chemotaxis and by lymphocyte responsiveness to phytohemaglutinin. All three cell types where shown to be simultaneously dysfunctional during  severe atopic flares. Chemotactic studies during clinical remissions disclosed notable improvement in cell migration. Serum IgE levels were elevated in each patient, but did not correlate with the degree of cutaneous anergy or altered leukocyte function.", "PMID": 962332}
{"id": "pubmed23n0032_4966", "title": "Topically applied thiabendazole in the treatment of scabies.", "content": "Forty patients were treated for scabies with a 10% suspension of thiabendazole applied externally. Thirty-two patients (80%) had a satisfactory response to one course of therapy, while a second course was necessary in six (15%). Two patients (5%) required intralesional injections of triamcinolone diacetate because of persistent scabious nodules.", "contents": "Topically applied thiabendazole in the treatment of scabies. Forty patients were treated for scabies with a 10% suspension of thiabendazole applied externally. Thirty-two patients (80%) had a satisfactory response to one course of therapy, while a second course was necessary in six (15%). Two patients (5%) required intralesional injections of triamcinolone diacetate because of persistent scabious nodules.", "PMID": 962333}
{"id": "pubmed23n0032_4967", "title": "Somatic generation of antibody diversity.", "content": "Hybridization of lambda-mRNAs to excess liver DNA yielded results compatible with gene reiteration frequencies of three or less. Purified mRNA from tumors producing structurally different lambda chains were used in competition hybridization experiments. An unlabeled lambda-mRNA competed with another, labelled mRNA to the same extent as homologous unlabelled lambda-mRNA. Mouse DNA was digested with Eco R-I restriction endonuclease and fractionated by gel electrophoresis. A DNA fragment carrying the V lambda-gene(s) was indentified in this digest. This fragment hybridized with lambda-mRNAs coding for two different lambdaV regions equally well. These results indicate that base sequence homology among lambda-mRNAs is so high that any lambda-mRNA should cross-hybridize with all or most of germ line V lambda genes. From amino acid sequence data, it is argued that there are probably more than 25 different lambdaV regions. Hence it is concluded that the number of germ line genes is too small to account for the diversity of lambda chains.", "contents": "Somatic generation of antibody diversity. Hybridization of lambda-mRNAs to excess liver DNA yielded results compatible with gene reiteration frequencies of three or less. Purified mRNA from tumors producing structurally different lambda chains were used in competition hybridization experiments. An unlabeled lambda-mRNA competed with another, labelled mRNA to the same extent as homologous unlabelled lambda-mRNA. Mouse DNA was digested with Eco R-I restriction endonuclease and fractionated by gel electrophoresis. A DNA fragment carrying the V lambda-gene(s) was indentified in this digest. This fragment hybridized with lambda-mRNAs coding for two different lambdaV regions equally well. These results indicate that base sequence homology among lambda-mRNAs is so high that any lambda-mRNA should cross-hybridize with all or most of germ line V lambda genes. From amino acid sequence data, it is argued that there are probably more than 25 different lambdaV regions. Hence it is concluded that the number of germ line genes is too small to account for the diversity of lambda chains.", "PMID": 962328}
{"id": "pubmed23n0032_4968", "title": "Vulval epidermoid cysts in the Igbos of Nigeria.", "content": "Epidermoid cysts are reported to be uncommon on the vulva. However, among the Igbos of Nigeria, the vulva was the most common site of such cysts in a series of specimens examined at a central laboratory. Vulval epidermoid cyssts probably arise as a result of traumatic implantation during ritual circumcision.", "contents": "Vulval epidermoid cysts in the Igbos of Nigeria. Epidermoid cysts are reported to be uncommon on the vulva. However, among the Igbos of Nigeria, the vulva was the most common site of such cysts in a series of specimens examined at a central laboratory. Vulval epidermoid cyssts probably arise as a result of traumatic implantation during ritual circumcision.", "PMID": 962334}
{"id": "pubmed23n0032_4969", "title": "Persulfate hair bleach reactions. Cutaneous and respiratory manifestations.", "content": "Ammonium persulfate is widely used to \"boost\" peroxide hair bleaches. These persulfates can produce a variety of cutaneous and respiratory responses, including allergic eczematous contact dermatitis, irritant dermatitis, localized edema, generalized urticaria, rhinitis, asthma, and syncope. Some of these reactions appear to be truly allergic while others appear to be due to the release of histamine on a nonallergic basis. Patch tests may be performed with 2% to 5% aqueous solution of ammonium persulfate. Scratch tests may result in asthma and syncope. In some patients, merely rubbing a saturated solution of ammonium persulfate into the skin will evoke a large urticarial wheal. Hairdressers should be made aware that these ammonium persulfate hair bleach preparations may provoke severe reactions and should seek medical attention if the client complains of severe itching, tingling, a burning sensation, hives, dizziness, or weakness.", "contents": "Persulfate hair bleach reactions. Cutaneous and respiratory manifestations. Ammonium persulfate is widely used to \"boost\" peroxide hair bleaches. These persulfates can produce a variety of cutaneous and respiratory responses, including allergic eczematous contact dermatitis, irritant dermatitis, localized edema, generalized urticaria, rhinitis, asthma, and syncope. Some of these reactions appear to be truly allergic while others appear to be due to the release of histamine on a nonallergic basis. Patch tests may be performed with 2% to 5% aqueous solution of ammonium persulfate. Scratch tests may result in asthma and syncope. In some patients, merely rubbing a saturated solution of ammonium persulfate into the skin will evoke a large urticarial wheal. Hairdressers should be made aware that these ammonium persulfate hair bleach preparations may provoke severe reactions and should seek medical attention if the client complains of severe itching, tingling, a burning sensation, hives, dizziness, or weakness.", "PMID": 962335}
{"id": "pubmed23n0032_4970", "title": "Epidermal nevus and rhabdomyosarcoma.", "content": "A child with an epidermal nevus was diagnosed at the age of 15 months as having an embryonal rhabdomyosarcoma of the bladder. This child also had pigmentary abnormalities characteristic of the epidermal nevus syndrome. The question is again asked whether patients with epidermal nevi have an increased incidence of tumors. It is suggested that the thorough evaluation of these patients as recommended by Solomon should also include an alertness for the development of earlier-than-anticipated neoplasms.", "contents": "Epidermal nevus and rhabdomyosarcoma. A child with an epidermal nevus was diagnosed at the age of 15 months as having an embryonal rhabdomyosarcoma of the bladder. This child also had pigmentary abnormalities characteristic of the epidermal nevus syndrome. The question is again asked whether patients with epidermal nevi have an increased incidence of tumors. It is suggested that the thorough evaluation of these patients as recommended by Solomon should also include an alertness for the development of earlier-than-anticipated neoplasms.", "PMID": 962336}
{"id": "pubmed23n0032_4971", "title": "Erosive adenomatosis of the nipple.", "content": "Erosive adenomatosis of the nipple is a rare, usually benign neoplasm of the major nipple ducts. Although morphologic features may distinguish it from Paget disease, histopathological differentiation is simple. Recognition of this tumor may spare needless mastectomy.", "contents": "Erosive adenomatosis of the nipple. Erosive adenomatosis of the nipple is a rare, usually benign neoplasm of the major nipple ducts. Although morphologic features may distinguish it from Paget disease, histopathological differentiation is simple. Recognition of this tumor may spare needless mastectomy.", "PMID": 962337}
{"id": "pubmed23n0032_4972", "title": "Perinevoid alopecia. An unusual variety of alopecia areata.", "content": "We report three cases of alopecia areata occurring around central, pigmented nevi. The histologic appearance of the central nevus was that of the halo nevus. Removal of the nevus did not appear to alter the natural course of the alopecia. Histochemical studies showed a substantial increase of acid mucopolysaccharides in the dermis in each case.", "contents": "Perinevoid alopecia. An unusual variety of alopecia areata. We report three cases of alopecia areata occurring around central, pigmented nevi. The histologic appearance of the central nevus was that of the halo nevus. Removal of the nevus did not appear to alter the natural course of the alopecia. Histochemical studies showed a substantial increase of acid mucopolysaccharides in the dermis in each case.", "PMID": 962338}
{"id": "pubmed23n0032_4973", "title": "Umbilical endometrioma (silent type).", "content": "Umbilical endometrioma is an uncommon entity and is rarely asymptomatic. A 24-year old black woman had an asymptomatic, spontaneous umbilical endometrioma. The lesion was excised and the diagnosis was histologically confirmed.", "contents": "Umbilical endometrioma (silent type). Umbilical endometrioma is an uncommon entity and is rarely asymptomatic. A 24-year old black woman had an asymptomatic, spontaneous umbilical endometrioma. The lesion was excised and the diagnosis was histologically confirmed.", "PMID": 962339}
{"id": "pubmed23n0032_4974", "title": "Steatocystoma multiplex.", "content": "Steatocystoma multiplex is a poorly understood clinical condition characterized by numerous cutaneous cysts. The lesions can be confused with milia, epidermal inclusion cysts, sebaceous cysts, dermoids, and the cysts of acne vulgaris. This patient has hypotrichosis and multiple milia-like cysts on the face and scalp, showing the histologic characteristics of steatocystoma multiplex.", "contents": "Steatocystoma multiplex. Steatocystoma multiplex is a poorly understood clinical condition characterized by numerous cutaneous cysts. The lesions can be confused with milia, epidermal inclusion cysts, sebaceous cysts, dermoids, and the cysts of acne vulgaris. This patient has hypotrichosis and multiple milia-like cysts on the face and scalp, showing the histologic characteristics of steatocystoma multiplex.", "PMID": 962340}
{"id": "pubmed23n0032_4975", "title": "Acquired hypertrichosis lanuginosa. A skin marker of internal malignancy.", "content": "A 54-year-old woman had acquired hypertrichosis lanuginosa. She developed excessive growth of lanugo-like hair all over her body two years prior to the sudden appearance of a rapidly growing, localized, solid mammary tumor. Of 12 published cases, nine have been associated with an internal malignant neoplasm and obviously, as in the present case, the cutaneous symptoms may serve as a skin marker by preceding the tumor diagnosis for up to two years.", "contents": "Acquired hypertrichosis lanuginosa. A skin marker of internal malignancy. A 54-year-old woman had acquired hypertrichosis lanuginosa. She developed excessive growth of lanugo-like hair all over her body two years prior to the sudden appearance of a rapidly growing, localized, solid mammary tumor. Of 12 published cases, nine have been associated with an internal malignant neoplasm and obviously, as in the present case, the cutaneous symptoms may serve as a skin marker by preceding the tumor diagnosis for up to two years.", "PMID": 962341}
{"id": "pubmed23n0032_4976", "title": "Phototoxic textile dermatitis (\"bikini dermatitis\").", "content": "Phototoxic textile dermatitis with subsequent hyperpigmentation developed in two patients after they wore bikini bathing suits. After extraction of the dye from the bathing suits, 15 fractions could be visualized by two-dimensional thin-layer chromatography. Two of these fractions are found in Disperse Blue 35, an anthraquinone dye known to give rise to occupational phototoxic dermatitis, but which, to our knowledge, has never been reported to cause dermatitis in consumers. One of the two fractions was also found to cause phototoxic reactions in normal subjects.", "contents": "Phototoxic textile dermatitis (\"bikini dermatitis\"). Phototoxic textile dermatitis with subsequent hyperpigmentation developed in two patients after they wore bikini bathing suits. After extraction of the dye from the bathing suits, 15 fractions could be visualized by two-dimensional thin-layer chromatography. Two of these fractions are found in Disperse Blue 35, an anthraquinone dye known to give rise to occupational phototoxic dermatitis, but which, to our knowledge, has never been reported to cause dermatitis in consumers. One of the two fractions was also found to cause phototoxic reactions in normal subjects.", "PMID": 962342}
{"id": "pubmed23n0032_4977", "title": "Hidrotic ectodermal dysplasia in a black mother and daughter.", "content": "Hidrotic ectodermal dysplasia is a rare genodermatosis with clinical hallmarks of alopecia, nail dystrophy, and palmoplantar hyperkeratosis. This report calls attention to the first reported cases, to our knowledge, of hidrotic ectodermal dysplasia in a black mother and daughter.", "contents": "Hidrotic ectodermal dysplasia in a black mother and daughter. Hidrotic ectodermal dysplasia is a rare genodermatosis with clinical hallmarks of alopecia, nail dystrophy, and palmoplantar hyperkeratosis. This report calls attention to the first reported cases, to our knowledge, of hidrotic ectodermal dysplasia in a black mother and daughter.", "PMID": 962343}
{"id": "pubmed23n0032_4978", "title": "Hepatitis and bone destruction as uncommon manifestations of early syphilis. Report of a case.", "content": "We describe a patient with a painful lytic lesion of the clavicle and a granuloma of the liver in late secondary syphilis. The classic \"moth-eaten\" alopecia of the scalp as well as eyebrows and eyelashes suggested syphilis, although an initial false-negative VDRL test for syphilis (prozone phenomenon) caused a delay in diagnosis. Bone as well as liver involvement in early syphilis may be more common than previously recognized.", "contents": "Hepatitis and bone destruction as uncommon manifestations of early syphilis. Report of a case. We describe a patient with a painful lytic lesion of the clavicle and a granuloma of the liver in late secondary syphilis. The classic \"moth-eaten\" alopecia of the scalp as well as eyebrows and eyelashes suggested syphilis, although an initial false-negative VDRL test for syphilis (prozone phenomenon) caused a delay in diagnosis. Bone as well as liver involvement in early syphilis may be more common than previously recognized.", "PMID": 962344}
{"id": "pubmed23n0032_4979", "title": "Inhibition of induction of human contact sensitization by topical glucocorticosteroids.", "content": "Ten healthy human volunteers were exposed to a primary sensitizing dose of 1 mg dinitrochlorobenzene (DNCB) by an open topical technique within an area that had been pretreated with a potent topical glucocorticosteroid compound. Quantitative elicitation testing was performed on the opposite side by an open patch test technique two weeks after the sensitizing application. One (10%) of the ten subjects became sensitized. A matched control group of ten subjects was similarly sensitized without steroid treatment. Eight (80%) of the ten became sensitized. One month later, five of the eight test subjects in whom sensitization had been prevented were retested in an identical fashion without steroid pretreatment, to determine if any degree of tolerance had been induced. All five subjects became sensitized. Topical glucocorticosteroids inhibited the development of sensitization to topically applied DNCB. Tolerance was not induced by this single process.", "contents": "Inhibition of induction of human contact sensitization by topical glucocorticosteroids. Ten healthy human volunteers were exposed to a primary sensitizing dose of 1 mg dinitrochlorobenzene (DNCB) by an open topical technique within an area that had been pretreated with a potent topical glucocorticosteroid compound. Quantitative elicitation testing was performed on the opposite side by an open patch test technique two weeks after the sensitizing application. One (10%) of the ten subjects became sensitized. A matched control group of ten subjects was similarly sensitized without steroid treatment. Eight (80%) of the ten became sensitized. One month later, five of the eight test subjects in whom sensitization had been prevented were retested in an identical fashion without steroid pretreatment, to determine if any degree of tolerance had been induced. All five subjects became sensitized. Topical glucocorticosteroids inhibited the development of sensitization to topically applied DNCB. Tolerance was not induced by this single process.", "PMID": 962352}
{"id": "pubmed23n0032_4980", "title": "Allergic contact dermatitis and stomatitis caused by a cinnamic aldehyde-flavored toothpaste.", "content": "A patient had acute stomatitis and dermatitis due to a popular toothpaste containing cinnamon oil flavor. Cinnamon cassia oil is known as a topical sensitizer and was demonstrated to be the offending allergen. Cinnamic aldehyde and related chemicals are used widely, so that patients having cinnamon allergy may be exposed to many sources. There is difficulty in diagnosing allergic contact stomatitis.", "contents": "Allergic contact dermatitis and stomatitis caused by a cinnamic aldehyde-flavored toothpaste. A patient had acute stomatitis and dermatitis due to a popular toothpaste containing cinnamon oil flavor. Cinnamon cassia oil is known as a topical sensitizer and was demonstrated to be the offending allergen. Cinnamic aldehyde and related chemicals are used widely, so that patients having cinnamon allergy may be exposed to many sources. There is difficulty in diagnosing allergic contact stomatitis.", "PMID": 962353}
{"id": "pubmed23n0032_4981", "title": "Dermatologists in the United States, 1968 through 1973.", "content": "From 1968 to 1973, the number of dermatologists in the United States per 100,000 population gradually increased from 1.6 to 1.8, with the West and Northeast regions showing relatively higher rates and the South and North Central regions showing relatively lower rates. The variation in rate, considered by geographic divisions, can be analyzed to identify areas in the United States that may be in need of additional dermatologists. Given the current number of dermatologists being certified by the American Board of Dermatology and given the current death rate, there appear to be a sufficient number of dermatologists to ensure future growth.", "contents": "Dermatologists in the United States, 1968 through 1973. From 1968 to 1973, the number of dermatologists in the United States per 100,000 population gradually increased from 1.6 to 1.8, with the West and Northeast regions showing relatively higher rates and the South and North Central regions showing relatively lower rates. The variation in rate, considered by geographic divisions, can be analyzed to identify areas in the United States that may be in need of additional dermatologists. Given the current number of dermatologists being certified by the American Board of Dermatology and given the current death rate, there appear to be a sufficient number of dermatologists to ensure future growth.", "PMID": 962354}
{"id": "pubmed23n0032_4982", "title": "Treatment of dermatomyositis in childhood.", "content": "Analysis of the response to corticosteroid therapy in a personal series of 8 consecutive cases of dermatomyositis in childhood shows that there are advantages in a moderate dosage, short-term treatment schedule, with gradual tapering of the dosage as soon as there is clinical improvement without waiting for full remission, and in trying to stop steroid therapy within six months rather than following the more prolonged regimen currently still in vogue. Clinical response is a more reliable guide to progress than serum enzyme levels. Review of published reports suggests that overtreatment with corticosteroids may be a factor in chronicity of the disease and failure of adequate long-term response.", "contents": "Treatment of dermatomyositis in childhood. Analysis of the response to corticosteroid therapy in a personal series of 8 consecutive cases of dermatomyositis in childhood shows that there are advantages in a moderate dosage, short-term treatment schedule, with gradual tapering of the dosage as soon as there is clinical improvement without waiting for full remission, and in trying to stop steroid therapy within six months rather than following the more prolonged regimen currently still in vogue. Clinical response is a more reliable guide to progress than serum enzyme levels. Review of published reports suggests that overtreatment with corticosteroids may be a factor in chronicity of the disease and failure of adequate long-term response.", "PMID": 962360}
{"id": "pubmed23n0032_4983", "title": "Association of cystic fibrosis with allergy.", "content": "Immediate skin hypersensitivity to various inhalant allergens was present in 59% of 123 children with cystic fibrosis (CF), a much higher percentage than in the general population. This is consistent with the idea that atopy arises as a result of impaired handling of antigen at mucosal surfaces. The allergic CF children had more chest infections, a worse chest x-ray appearance, and lower peak expiratory flow rates. Allergic diseases were also frequent in the CF obligate heterozygotes (32% of mothers and 26% of fathers). It is suggested that the heterozygotes may also have a mucosal abnormality resulting in defective antigen handling.", "contents": "Association of cystic fibrosis with allergy. Immediate skin hypersensitivity to various inhalant allergens was present in 59% of 123 children with cystic fibrosis (CF), a much higher percentage than in the general population. This is consistent with the idea that atopy arises as a result of impaired handling of antigen at mucosal surfaces. The allergic CF children had more chest infections, a worse chest x-ray appearance, and lower peak expiratory flow rates. Allergic diseases were also frequent in the CF obligate heterozygotes (32% of mothers and 26% of fathers). It is suggested that the heterozygotes may also have a mucosal abnormality resulting in defective antigen handling.", "PMID": 962361}
{"id": "pubmed23n0032_4984", "title": "Long-term assessment of children exposed in utero to carbimazole.", "content": "Twenty-five children aged 3 to 13 years who had been exposed in utero to carbimazole were assessed physically and psychologically to evaluate the long-term effects of the drug on growth and development. 2 children had congenital malformations but all had normal pituitary-thyroid function and appeared to have grown and developed normally.", "contents": "Long-term assessment of children exposed in utero to carbimazole. Twenty-five children aged 3 to 13 years who had been exposed in utero to carbimazole were assessed physically and psychologically to evaluate the long-term effects of the drug on growth and development. 2 children had congenital malformations but all had normal pituitary-thyroid function and appeared to have grown and developed normally.", "PMID": 962362}
{"id": "pubmed23n0032_4985", "title": "Plasma renin activity and aldosterone concentration in the human newborn.", "content": "Twenty-one healthy newborn infants whose mothers had normal pregnancies and deliveries were studied. Plasma renin activity (PRA) and plasma aldosterone concentration (PAldo) were measured by semimicro methods in cord blood and on the 6th day of life. PRA and PAldo were increased in cord blood compared with values previously found in healthy infants between 1 month and 1 year of age. There was a twofold reduction in both values by the 6th day. PRA and PAldo were positively correlated when cord blood and 6th-day values were considered as a whole, but no significant correlations were found between either cord blood PRA or cord blood PAldo and their respective values on the 6th day. The PRA and PAldo findings were not related to birthweight, the infant's sex, weight change, 6th-day urinary sodium, or blood pressure measurements. The type of milk feed did not influence PRA but PAldo was slightly greater in bottle-fed babies on the 6 day. These observations confirmed that the inverse relationship between PRA, PAldo, and age extends into the neonatal period but they suggest that the balance of factors controlling these variables at birth is different to that operating at the age of 6 days.", "contents": "Plasma renin activity and aldosterone concentration in the human newborn. Twenty-one healthy newborn infants whose mothers had normal pregnancies and deliveries were studied. Plasma renin activity (PRA) and plasma aldosterone concentration (PAldo) were measured by semimicro methods in cord blood and on the 6th day of life. PRA and PAldo were increased in cord blood compared with values previously found in healthy infants between 1 month and 1 year of age. There was a twofold reduction in both values by the 6th day. PRA and PAldo were positively correlated when cord blood and 6th-day values were considered as a whole, but no significant correlations were found between either cord blood PRA or cord blood PAldo and their respective values on the 6th day. The PRA and PAldo findings were not related to birthweight, the infant's sex, weight change, 6th-day urinary sodium, or blood pressure measurements. The type of milk feed did not influence PRA but PAldo was slightly greater in bottle-fed babies on the 6 day. These observations confirmed that the inverse relationship between PRA, PAldo, and age extends into the neonatal period but they suggest that the balance of factors controlling these variables at birth is different to that operating at the age of 6 days.", "PMID": 962363}
{"id": "pubmed23n0032_4986", "title": "Follow-up of 13 children after ureterosigmoidostomy.", "content": "Follow-up of 13 children who had had a ureterosigmoid anastomosis 3 1/2 to 10 years previously and whose initial urogram had been satisfactory, showed that growth was normal and that there was no serious metabolic disorder. In particular whole-body potassium did not differ significantly from normal values (as given by Langham, 1961). Asymptomatic urinary infection is the chief hazard in these cases but is difficult to diagnose and may lead to progressive dilatation of the ureters.", "contents": "Follow-up of 13 children after ureterosigmoidostomy. Follow-up of 13 children who had had a ureterosigmoid anastomosis 3 1/2 to 10 years previously and whose initial urogram had been satisfactory, showed that growth was normal and that there was no serious metabolic disorder. In particular whole-body potassium did not differ significantly from normal values (as given by Langham, 1961). Asymptomatic urinary infection is the chief hazard in these cases but is difficult to diagnose and may lead to progressive dilatation of the ureters.", "PMID": 962364}
{"id": "pubmed23n0032_4987", "title": "Achondrogenesis type I. A familial subvariant?", "content": "The clinical, pathological, and radiological features of 2 male sibs with a severe and lethal form of micromelic dwarfism are desribed. The family also includes 2 normal sibs. The histological and radiological appearances suggested a diagnosis of achondrogenesis type I, but the markedly deficient ossification of the skull and the presence of intrauterine rib fractures were atypical. These changes have been observed in two other families with 2 or more infants with suspected achondrogenesis, raising the possibility that these familial cases may be a subvariant of achondrogesis or even a distinct disease entity. The disease appears to be inherited as an autosomal recessive and death occurs shortly after birth because of severe pulmonary hypoplasia.", "contents": "Achondrogenesis type I. A familial subvariant? The clinical, pathological, and radiological features of 2 male sibs with a severe and lethal form of micromelic dwarfism are desribed. The family also includes 2 normal sibs. The histological and radiological appearances suggested a diagnosis of achondrogenesis type I, but the markedly deficient ossification of the skull and the presence of intrauterine rib fractures were atypical. These changes have been observed in two other families with 2 or more infants with suspected achondrogenesis, raising the possibility that these familial cases may be a subvariant of achondrogesis or even a distinct disease entity. The disease appears to be inherited as an autosomal recessive and death occurs shortly after birth because of severe pulmonary hypoplasia.", "PMID": 962365}
{"id": "pubmed23n0032_4988", "title": "Homozygous cystinuria and the oculo-cerebro-renal dystrophy of Lowe in same family.", "content": "The mother and daughter in a family had homozygous cystinuria and were also heterozygous carriers of the oculo-cerebro-renal dystrophy of Lowe. The daughter was also epileptic. The son had Lowe's syndrome and the father an increased urinary excretion of cystine and lysine. This evidence together with other case reports suggests that the defect in cystinuria and that of Lowe's syndrome may be connected.", "contents": "Homozygous cystinuria and the oculo-cerebro-renal dystrophy of Lowe in same family. The mother and daughter in a family had homozygous cystinuria and were also heterozygous carriers of the oculo-cerebro-renal dystrophy of Lowe. The daughter was also epileptic. The son had Lowe's syndrome and the father an increased urinary excretion of cystine and lysine. This evidence together with other case reports suggests that the defect in cystinuria and that of Lowe's syndrome may be connected.", "PMID": 962366}
{"id": "pubmed23n0032_4989", "title": "Pre- and postoperative growth in persistent ductus arteriosus.", "content": "Children with a persistent ductus arteriosus requiring ligation under 6 months of age show evidence of intrauterine growth retardation. By the time of operation the weight centile of 17 (71%) of 24 such infants had fallen, but 20(84%) of the 24 gained weight postoperatively. Those who did not so were either large-for-dates infants growing towards the normal or small-for-dates infants who remained small postoperatively.", "contents": "Pre- and postoperative growth in persistent ductus arteriosus. Children with a persistent ductus arteriosus requiring ligation under 6 months of age show evidence of intrauterine growth retardation. By the time of operation the weight centile of 17 (71%) of 24 such infants had fallen, but 20(84%) of the 24 gained weight postoperatively. Those who did not so were either large-for-dates infants growing towards the normal or small-for-dates infants who remained small postoperatively.", "PMID": 962367}
{"id": "pubmed23n0032_4990", "title": "Neonatal thyrotoxicosis is associated with transplacental passage of human thyroid stimulating immunoglobulin (HTSI).", "content": "A woman who developed thyrotoxicosis during pregnancy had a high level of HTSI at the time of delivery. The baby had high levels of HTSI initially but, unlike the mother, the levels fell, giving a half-life of HTSI of 10 days.", "contents": "Neonatal thyrotoxicosis is associated with transplacental passage of human thyroid stimulating immunoglobulin (HTSI). A woman who developed thyrotoxicosis during pregnancy had a high level of HTSI at the time of delivery. The baby had high levels of HTSI initially but, unlike the mother, the levels fell, giving a half-life of HTSI of 10 days.", "PMID": 962368}
{"id": "pubmed23n0032_4991", "title": "Hypoaldosteronism in three sibs due to 18-dehydrogenase deficiency.", "content": "Three sibs all presented in the early neonatal period with a salt-losing syndrome. The salt-losing form of congenital adrenal hyperplasia was diagnosed and appropriate treatment with glucocorticosteroids, mineralocorticosteroids, and additional dietary salt started. Although early life was maintained with difficulty, with age all 3 children required decreasing amounts of replacement steroids to maintain normal plasma electrolyte balance. They were reinvestigated at the ages of 15 years and 8 years (twins), when cortisol synthesis and metabolism proved normal, but aldosterone synthesis was blocked by deficiency of 18-dehydrogenase. Rational treatment of these cases of a salt-losing syndrome in which aldosterone synthesis alone is blocked due to lack of the enzyme 18-dehydrogenase requires the administration of a mineralocorticosteroid drug only. Since deoxycorticosterone (acetate or pivalate) requires intramuscular administration, as life-long therapy oral fludrocortisone is preferable. Although fludrocortisone has glucocorticoid activity, the \"hydrocortisone equivalent\" effect of the small dosage used was unlikely to inhibit either pituitary corticotrophin or growth hormone production.", "contents": "Hypoaldosteronism in three sibs due to 18-dehydrogenase deficiency. Three sibs all presented in the early neonatal period with a salt-losing syndrome. The salt-losing form of congenital adrenal hyperplasia was diagnosed and appropriate treatment with glucocorticosteroids, mineralocorticosteroids, and additional dietary salt started. Although early life was maintained with difficulty, with age all 3 children required decreasing amounts of replacement steroids to maintain normal plasma electrolyte balance. They were reinvestigated at the ages of 15 years and 8 years (twins), when cortisol synthesis and metabolism proved normal, but aldosterone synthesis was blocked by deficiency of 18-dehydrogenase. Rational treatment of these cases of a salt-losing syndrome in which aldosterone synthesis alone is blocked due to lack of the enzyme 18-dehydrogenase requires the administration of a mineralocorticosteroid drug only. Since deoxycorticosterone (acetate or pivalate) requires intramuscular administration, as life-long therapy oral fludrocortisone is preferable. Although fludrocortisone has glucocorticoid activity, the \"hydrocortisone equivalent\" effect of the small dosage used was unlikely to inhibit either pituitary corticotrophin or growth hormone production.", "PMID": 962369}
{"id": "pubmed23n0032_4992", "title": "Lack of breast feeding and early weaning in infants of Asian immigrants to Wolverhampton.", "content": "Fifty Asian immigrant mothers who would have expected to breast feed their infants had they remained in rural Asia were studied. There was a striking reduction in the incidence and duration of breast feeding on arrival in the United Kingdom, and a fall in the age of weaning. The availability of an alternative to human milk is the most important factor reducing the incidence of breast feeding. Only 2 (4%) of the 46 infants followed prospectively were breast fed. Reasons for not breast feeding were sought and the results indicated that the majority of mothers were frightened, misinformed, or apathetic about breast feeding. If breast feeding is to be promoted, antenatal education and encouragement is essential. The advantages of human milk need to be stressed. Potentially serious mistakes occurred in preparing bottle feeds, and vitamin supplements were often inadequate. Later weaning could be encouraged by the staff of well baby clinics.", "contents": "Lack of breast feeding and early weaning in infants of Asian immigrants to Wolverhampton. Fifty Asian immigrant mothers who would have expected to breast feed their infants had they remained in rural Asia were studied. There was a striking reduction in the incidence and duration of breast feeding on arrival in the United Kingdom, and a fall in the age of weaning. The availability of an alternative to human milk is the most important factor reducing the incidence of breast feeding. Only 2 (4%) of the 46 infants followed prospectively were breast fed. Reasons for not breast feeding were sought and the results indicated that the majority of mothers were frightened, misinformed, or apathetic about breast feeding. If breast feeding is to be promoted, antenatal education and encouragement is essential. The advantages of human milk need to be stressed. Potentially serious mistakes occurred in preparing bottle feeds, and vitamin supplements were often inadequate. Later weaning could be encouraged by the staff of well baby clinics.", "PMID": 962370}
{"id": "pubmed23n0032_4993", "title": "Congenital paroxysmal atrial tachycardia.", "content": "Ten infants who had paroxysmal atrial tachycardia in utero or at birth are reported. Because of apparent fetal distress, caesarean section was performed in 4 cases and labour was induced in 1. Birthweight was generally large for gestational age. Severe ascites and hydrops at birth were manifestations of cardiac failure. Atrial flutter was recorded in 4 infants and supraventricular tachycardia in 5. The WoLff-Parkinson-White syndrome became evident later in 2. Digoxin was given to all 10 infants, and cardioversion was required and was effective in 4. Known recurrences in childhood have occurred in only 1 patient. Congenital atrial tachyarrhythmias may be commoner than generally believed, and fetal electrocardiography may help to avoid unnecessary termination of pregnancy. Blood sugar determinations are important, since neonatal hypoglycaemia was found. Cardioversion should be performed promptly in severely ill infants or if there is no response to digoxin. Care is required to avoid digoxin toxicity.", "contents": "Congenital paroxysmal atrial tachycardia. Ten infants who had paroxysmal atrial tachycardia in utero or at birth are reported. Because of apparent fetal distress, caesarean section was performed in 4 cases and labour was induced in 1. Birthweight was generally large for gestational age. Severe ascites and hydrops at birth were manifestations of cardiac failure. Atrial flutter was recorded in 4 infants and supraventricular tachycardia in 5. The WoLff-Parkinson-White syndrome became evident later in 2. Digoxin was given to all 10 infants, and cardioversion was required and was effective in 4. Known recurrences in childhood have occurred in only 1 patient. Congenital atrial tachyarrhythmias may be commoner than generally believed, and fetal electrocardiography may help to avoid unnecessary termination of pregnancy. Blood sugar determinations are important, since neonatal hypoglycaemia was found. Cardioversion should be performed promptly in severely ill infants or if there is no response to digoxin. Care is required to avoid digoxin toxicity.", "PMID": 962371}
{"id": "pubmed23n0032_4994", "title": "Arthrogryposis multiplex congenita. Search for prenatal factors in 66 sporadic cases.", "content": "In a family and epidemiological survey of 66 cases of arthrogryposis multiplex congenita all cases were found to be sporadic and no family association with clubfoot, congenital dislocation of the hip, or hereditary neuromuscular disease was found. The mothers were significantly older than average. Oligohydramnios was noted in only one-third of cases but many other complications of pregnancy, including probable attempts at abortion, had occurred. It is likely that most cases of arthrogryposis are nongenetic and result from a defective intrauterine environment, whether hormonal, vascular, mechanical, or possibly infective.", "contents": "Arthrogryposis multiplex congenita. Search for prenatal factors in 66 sporadic cases. In a family and epidemiological survey of 66 cases of arthrogryposis multiplex congenita all cases were found to be sporadic and no family association with clubfoot, congenital dislocation of the hip, or hereditary neuromuscular disease was found. The mothers were significantly older than average. Oligohydramnios was noted in only one-third of cases but many other complications of pregnancy, including probable attempts at abortion, had occurred. It is likely that most cases of arthrogryposis are nongenetic and result from a defective intrauterine environment, whether hormonal, vascular, mechanical, or possibly infective.", "PMID": 962372}
{"id": "pubmed23n0032_4995", "title": "Alterations in head shape of newborn infants after caesarean section or vaginal delivery.", "content": "Alterations of head shape in preterm, small-for-dates, and term normal infants were studied by measuring occipitofrontal circumference (OFC), biparietal diameter (BPD), and occipitofrontal diameter (OFD) at intervals after birth. In 9 preterm infants born by elective caesarean section ther was a 5-2% reduction in BPD and 2-0% reduction in OFC at the age of 7 days. In 18 term infants born by elective caesarean section these changes were 2-4% and 0% respectively in BPD and OFC. In 25 preterm infants born by vertex vaginal delivery there was a significant fall in OFC of 0-7% at the age of 7 days and of 2-4% in BPD, but no significant change in OFD. In 19 small-for-dates infants born vaginally OFC increased 1-0% and OFD 2-7% at 7 days, but BPD decreased 2-5%. After the first week all three measurements increased in both groups of vaginal deliveries. The results show that shrinkage and biparietal flattening of the skull occur during the first week of life in preterm and term infants born by caesarean section and in preterm infants born vaginally. This fact should be borne in mind when comparing the measurements of an infant's head size with published norms.", "contents": "Alterations in head shape of newborn infants after caesarean section or vaginal delivery. Alterations of head shape in preterm, small-for-dates, and term normal infants were studied by measuring occipitofrontal circumference (OFC), biparietal diameter (BPD), and occipitofrontal diameter (OFD) at intervals after birth. In 9 preterm infants born by elective caesarean section ther was a 5-2% reduction in BPD and 2-0% reduction in OFC at the age of 7 days. In 18 term infants born by elective caesarean section these changes were 2-4% and 0% respectively in BPD and OFC. In 25 preterm infants born by vertex vaginal delivery there was a significant fall in OFC of 0-7% at the age of 7 days and of 2-4% in BPD, but no significant change in OFD. In 19 small-for-dates infants born vaginally OFC increased 1-0% and OFD 2-7% at 7 days, but BPD decreased 2-5%. After the first week all three measurements increased in both groups of vaginal deliveries. The results show that shrinkage and biparietal flattening of the skull occur during the first week of life in preterm and term infants born by caesarean section and in preterm infants born vaginally. This fact should be borne in mind when comparing the measurements of an infant's head size with published norms.", "PMID": 962373}
{"id": "pubmed23n0032_4996", "title": "Echovirus 19 infection in infants under six months.", "content": "Twenty-four infants under 6 months infected with echovirus 19 are described, They were the youngest of the many children admitted to hospitals in Newcastle and Gateshead during an epidemic in the north-east of England in 1974. Generally, the younger the child the more severe the illness, which affected the upper respiratory tract, the gut, the skin, and the meninges, and sometimes caused as state of collapse resembling septicaemic shock. Polymorphonuclear pleocystosis of the cerebrospinal fluid (CSF) sometimes suggested bacterial meningitis, so that antibiotics were given in 38% of cases. The virus was recovered with a high success rate from nasopharyngeal secretions, CSF, and stool.", "contents": "Echovirus 19 infection in infants under six months. Twenty-four infants under 6 months infected with echovirus 19 are described, They were the youngest of the many children admitted to hospitals in Newcastle and Gateshead during an epidemic in the north-east of England in 1974. Generally, the younger the child the more severe the illness, which affected the upper respiratory tract, the gut, the skin, and the meninges, and sometimes caused as state of collapse resembling septicaemic shock. Polymorphonuclear pleocystosis of the cerebrospinal fluid (CSF) sometimes suggested bacterial meningitis, so that antibiotics were given in 38% of cases. The virus was recovered with a high success rate from nasopharyngeal secretions, CSF, and stool.", "PMID": 962374}
{"id": "pubmed23n0032_4997", "title": "Severe echo 19 virus infection in a neonatal unit.", "content": "An epidemic of echo 19 virus infection in a neonatal unit affecting 12 babies with one death is described. With one exception it was confined to the neonatal unit and medical and nursing staff were also affected. The unit was closed for 9 days, then was disinfected, and there was no recurrence.", "contents": "Severe echo 19 virus infection in a neonatal unit. An epidemic of echo 19 virus infection in a neonatal unit affecting 12 babies with one death is described. With one exception it was confined to the neonatal unit and medical and nursing staff were also affected. The unit was closed for 9 days, then was disinfected, and there was no recurrence.", "PMID": 962375}
{"id": "pubmed23n0032_4998", "title": "Organic aciduria. Treatable cause of floppy infant syndrome.", "content": "A floppy infant is described who has an inborn error of organic acid metabolism due to defective activity of the enzyme beta-methylcrotonyl CoA carboxylase. She presented with hyperventilation, hypotonia, and regression of motor and intellectual development. She responded to treatment with biotin.", "contents": "Organic aciduria. Treatable cause of floppy infant syndrome. A floppy infant is described who has an inborn error of organic acid metabolism due to defective activity of the enzyme beta-methylcrotonyl CoA carboxylase. She presented with hyperventilation, hypotonia, and regression of motor and intellectual development. She responded to treatment with biotin.", "PMID": 962376}
{"id": "pubmed23n0032_4999", "title": "Balanced translocation, impaired sperm motility, and offspring anomaly.", "content": "An infant with multiple physical abnormalities, failure to thrive, and mental deficiency, all probably due to a 22/11 chromosomal translocation is described, and the implications of inducing pregnancies in childless couples are discussed.", "contents": "Balanced translocation, impaired sperm motility, and offspring anomaly. An infant with multiple physical abnormalities, failure to thrive, and mental deficiency, all probably due to a 22/11 chromosomal translocation is described, and the implications of inducing pregnancies in childless couples are discussed.", "PMID": 962377}
{"id": "pubmed23n0032_5000", "title": "Enhancement of sperm acrosin activity by glycerol-pretreatment - quantitative estimations.", "content": "Acetic acid treatment of spermatozoa, a method suitable for optimal extraction of the acrosomal proteinase acrosin, was used to show the influence of glycerol on sperm acrosin activity. Short-time pretreatment of semen samples with glycerol in concentrations up to 35% (v/v) caused a 1.5-2.5 fold increase in sperm acrosin activity. Higher glycerol concentrations caused a decrease in sperm acrosin activity due to leakage of the enzyme into the suspension medium. A further increase in sperm acrosin activity was observed during aging of semen in the presence of glycerol. In this case, the height of the increase depends on the glycerol concentration applied as well as incubation temperature and time. The acrosin activation pattern induced by glycerol was not influenced by proteinase (acrosin) inhibitors. On the other hand, addition of glycerol to spermatozoa from which the seminal plasma had been removed and substituted by physiological saline caused only a small increase in sperm acrosin activity. This indicates the occurrence of a seminal plasma factor which is either stimulated (activated) by glycerol or which can penetrate the membranes and subsequently activate acrosin only in the presence of glycerol. This seminal plasma factor was not consumed during activation and could be transfered to another sperm sample. However, a protecting influence of such a factor on the sperm head membranes and thus an indirect activation effect, i.e. better extractibility of acrosin, has also to be considered. The glycerol-induced rise of the acrosin activity is not caused by reversible conformational changes of the enzyme molecules: acrosin activity was not diminished if glycerol was removed from semen samples or extracts later on. The possibility that the observed increase in BAEE-splitting activity is due to a so far unknown proteinase may be excluded: the activity completely disappeared by neutralization due to formation of the acrosin-inhibitor complex and appeared again by acidification, a well known characteristic of acrosin and its inhibitors. Factors which are probably responsible for the glycerol-induced activation of acrosin-membrane effects and the activation of a precursor from of acrosin-are discussed.", "contents": "Enhancement of sperm acrosin activity by glycerol-pretreatment - quantitative estimations. Acetic acid treatment of spermatozoa, a method suitable for optimal extraction of the acrosomal proteinase acrosin, was used to show the influence of glycerol on sperm acrosin activity. Short-time pretreatment of semen samples with glycerol in concentrations up to 35% (v/v) caused a 1.5-2.5 fold increase in sperm acrosin activity. Higher glycerol concentrations caused a decrease in sperm acrosin activity due to leakage of the enzyme into the suspension medium. A further increase in sperm acrosin activity was observed during aging of semen in the presence of glycerol. In this case, the height of the increase depends on the glycerol concentration applied as well as incubation temperature and time. The acrosin activation pattern induced by glycerol was not influenced by proteinase (acrosin) inhibitors. On the other hand, addition of glycerol to spermatozoa from which the seminal plasma had been removed and substituted by physiological saline caused only a small increase in sperm acrosin activity. This indicates the occurrence of a seminal plasma factor which is either stimulated (activated) by glycerol or which can penetrate the membranes and subsequently activate acrosin only in the presence of glycerol. This seminal plasma factor was not consumed during activation and could be transfered to another sperm sample. However, a protecting influence of such a factor on the sperm head membranes and thus an indirect activation effect, i.e. better extractibility of acrosin, has also to be considered. The glycerol-induced rise of the acrosin activity is not caused by reversible conformational changes of the enzyme molecules: acrosin activity was not diminished if glycerol was removed from semen samples or extracts later on. The possibility that the observed increase in BAEE-splitting activity is due to a so far unknown proteinase may be excluded: the activity completely disappeared by neutralization due to formation of the acrosin-inhibitor complex and appeared again by acidification, a well known characteristic of acrosin and its inhibitors. Factors which are probably responsible for the glycerol-induced activation of acrosin-membrane effects and the activation of a precursor from of acrosin-are discussed.", "PMID": 962379}
{"id": "pubmed23n0032_5001", "title": "[Immunological investigations in lichen planus patients (author's transl)].", "content": "30 patients suffering from generalized lichen planus (= L.p.) have been examined by the following methods in order to more precise information regarding their immune-status: 1. Quantitative determination of immuno-globulins including complement fraction (beta 1C/A) 2. Intracutaneous tests with recall-antigens 3. Investigation of cutaneous sensitivity to DNCB 4. Lymphocytemigrationinhibition- and Lymphocytetransformationstests. The results obtained were not statistically significant when comparing the results of this investigation in patients suffering from L.p. with the control group. Therefore, the theory that immunological processes are the basis of these occurences is still under discussion. Further studies applying more specific methods should be conducted in order to clarify this problem.", "contents": "[Immunological investigations in lichen planus patients (author's transl)]. 30 patients suffering from generalized lichen planus (= L.p.) have been examined by the following methods in order to more precise information regarding their immune-status: 1. Quantitative determination of immuno-globulins including complement fraction (beta 1C/A) 2. Intracutaneous tests with recall-antigens 3. Investigation of cutaneous sensitivity to DNCB 4. Lymphocytemigrationinhibition- and Lymphocytetransformationstests. The results obtained were not statistically significant when comparing the results of this investigation in patients suffering from L.p. with the control group. Therefore, the theory that immunological processes are the basis of these occurences is still under discussion. Further studies applying more specific methods should be conducted in order to clarify this problem.", "PMID": 962380}
{"id": "pubmed23n0032_5002", "title": "[Morphological and biochemical investigations of hairs in inborn errors of amino acid metabolism (author's transl)].", "content": "The influence of inborn errors of metabolism on the amino acid content, the structure and growth of human hair has been studied in patients suffering from Phenylketonuria, Cystinosis, Homocystinuria and Tyrosinosis. Examiniation of hairs under the scanning electron microscope reveals defects and abnormalities such as a plicated pattern of the cuticula in patients with Phenylketonuria and Cystinosis. The amino acid content of the hydrolized hair keratin of all patients was within normal range and did not reveal significant changes of phenylalanine, cystine, homocystine, methionine or tyrosine. Disturbance in hair growth was determined by evaluation of standardized hair root samples. The results indicate an increase in hair root atrophy with increasing severity of the disorder of amino acid metabolism.", "contents": "[Morphological and biochemical investigations of hairs in inborn errors of amino acid metabolism (author's transl)]. The influence of inborn errors of metabolism on the amino acid content, the structure and growth of human hair has been studied in patients suffering from Phenylketonuria, Cystinosis, Homocystinuria and Tyrosinosis. Examiniation of hairs under the scanning electron microscope reveals defects and abnormalities such as a plicated pattern of the cuticula in patients with Phenylketonuria and Cystinosis. The amino acid content of the hydrolized hair keratin of all patients was within normal range and did not reveal significant changes of phenylalanine, cystine, homocystine, methionine or tyrosine. Disturbance in hair growth was determined by evaluation of standardized hair root samples. The results indicate an increase in hair root atrophy with increasing severity of the disorder of amino acid metabolism.", "PMID": 962381}
{"id": "pubmed23n0032_5003", "title": "[Penetrations kinetics and distribution of topically applied estrogens (author's transl)].", "content": "The penetration into the single layers of human skin in vitro of 17 alpha-estradiol, 17 beta-estradiol and estriol was investigated. The radiolabeled substances were incorporated into 4 standard ointments and into an alcoholic solution mixture. After application to the skin and after different penetration periods, the horny layer was taken off by adhesive tape stripping. The epidermis and the dermis were separated by slicing them down parallel to the skin surface in a freeze microtome. In each single layer, the amount of substance was determined and calculated relative to the applied quantity and in absolute concentrations (mug per tissue weight and molarity). Besides the expected dependence of the penetration on the type of ointment, there is a distinct dependence on the chemical structure: estriol penetrates considerably slower and in less concentrations into the living layers of the human skin than the estradiols. Furthermore, estriol reaches the dermis only in low concentrations so that this substance may be termed epidermotropic. 17 alpha-estriol which has only weak sexhormone properties in humans penetrates as well as the sexhormone 17 beta-estradiol.", "contents": "[Penetrations kinetics and distribution of topically applied estrogens (author's transl)]. The penetration into the single layers of human skin in vitro of 17 alpha-estradiol, 17 beta-estradiol and estriol was investigated. The radiolabeled substances were incorporated into 4 standard ointments and into an alcoholic solution mixture. After application to the skin and after different penetration periods, the horny layer was taken off by adhesive tape stripping. The epidermis and the dermis were separated by slicing them down parallel to the skin surface in a freeze microtome. In each single layer, the amount of substance was determined and calculated relative to the applied quantity and in absolute concentrations (mug per tissue weight and molarity). Besides the expected dependence of the penetration on the type of ointment, there is a distinct dependence on the chemical structure: estriol penetrates considerably slower and in less concentrations into the living layers of the human skin than the estradiols. Furthermore, estriol reaches the dermis only in low concentrations so that this substance may be termed epidermotropic. 17 alpha-estriol which has only weak sexhormone properties in humans penetrates as well as the sexhormone 17 beta-estradiol.", "PMID": 962382}
{"id": "pubmed23n0032_5004", "title": "Separation, isolation, and amino acid composition of the albumin-like fraction from psoriatic scales.", "content": "The albumin-like fraction of psoriatic scales was isolated either directly by slab gel electroporesis or after previous treatment with polyethylene glycol6000. After both isolation procedures the amino acid composition was determined. Both proteins were rich of glutamic acid, glycine, and alanine. On the other hand both contained very small amounts of cysteine and tyrosine. Only after the direct isolation procedure the amino-sugar galactosamine could be detected.", "contents": "Separation, isolation, and amino acid composition of the albumin-like fraction from psoriatic scales. The albumin-like fraction of psoriatic scales was isolated either directly by slab gel electroporesis or after previous treatment with polyethylene glycol6000. After both isolation procedures the amino acid composition was determined. Both proteins were rich of glutamic acid, glycine, and alanine. On the other hand both contained very small amounts of cysteine and tyrosine. Only after the direct isolation procedure the amino-sugar galactosamine could be detected.", "PMID": 962383}
{"id": "pubmed23n0032_5005", "title": "Dopa-positive cells and melanin in basal cell epithelioma (BCE).", "content": "In 47 out of 61 bce's, melanocytes were detectable with the aid of Dopa-reaction. In 24 of these cases, the percentage of tumour cells was less 5%, and in 23 it was more. The melanocytes preferred the periphery of the epithelial tumour formations. Only when the proportion of melanocytes exceeded 5% were they also to be found in the central parts of the tumours. Generally the melanocytes were more numerous in the upper parts of tumours, next to the epidermis. Our findings strengthen the opinion, that bce is a tumour of the primary hair germ.", "contents": "Dopa-positive cells and melanin in basal cell epithelioma (BCE). In 47 out of 61 bce's, melanocytes were detectable with the aid of Dopa-reaction. In 24 of these cases, the percentage of tumour cells was less 5%, and in 23 it was more. The melanocytes preferred the periphery of the epithelial tumour formations. Only when the proportion of melanocytes exceeded 5% were they also to be found in the central parts of the tumours. Generally the melanocytes were more numerous in the upper parts of tumours, next to the epidermis. Our findings strengthen the opinion, that bce is a tumour of the primary hair germ.", "PMID": 962384}
{"id": "pubmed23n0032_5006", "title": "Enzymes of carbohydrate metabolism in naevoid basal-cell epithelioma.", "content": "The activities of 27 enzymes of carbohydrate metabolism and the proportions of lactate dehydrogenase isoenzymes were determined in epidermis, in superficial epitheliomas and in several solid tumours biopsied from a patient with basal-cell naevus syndrome. The activity patterns varied from that of normal epidermis to that found in basal-cell epitheliomas. Thickened epidermis, superficial epitheliomas and some of the solid tumours presented an intermediate pattern. Histologically similar lesions sometimes differed markedly in their enzyme activities.", "contents": "Enzymes of carbohydrate metabolism in naevoid basal-cell epithelioma. The activities of 27 enzymes of carbohydrate metabolism and the proportions of lactate dehydrogenase isoenzymes were determined in epidermis, in superficial epitheliomas and in several solid tumours biopsied from a patient with basal-cell naevus syndrome. The activity patterns varied from that of normal epidermis to that found in basal-cell epitheliomas. Thickened epidermis, superficial epitheliomas and some of the solid tumours presented an intermediate pattern. Histologically similar lesions sometimes differed markedly in their enzyme activities.", "PMID": 962385}
{"id": "pubmed23n0032_5007", "title": "[Experimental inoculation of Leishmaniasis tropical from man to man (author's transl)].", "content": "Experimental transformation of leishmaniasis from man to man was demonstrated. The infection occurs through direct inoculation of the leishmania organism in the amastigote form: from an ulcer of a leishmania papule some materials were obtained and were inoculated intradermally in the thigh of one of the authors. Within weeks a red papule has developed in the place of inoculation from which the microorganisms were frequenctly demonstrated. The papule was cured spontaneously within 70 weeks under a formation of a flat pigmented scar. In the electronenmicroscopy the microorganisms were seen to be located intracellular. The microorganism had a double outer membrane, an oval nucleus, and a kinetoplast out of which (in the phlebotomus fly or in the culture) the future flagellum would be formed.", "contents": "[Experimental inoculation of Leishmaniasis tropical from man to man (author's transl)]. Experimental transformation of leishmaniasis from man to man was demonstrated. The infection occurs through direct inoculation of the leishmania organism in the amastigote form: from an ulcer of a leishmania papule some materials were obtained and were inoculated intradermally in the thigh of one of the authors. Within weeks a red papule has developed in the place of inoculation from which the microorganisms were frequenctly demonstrated. The papule was cured spontaneously within 70 weeks under a formation of a flat pigmented scar. In the electronenmicroscopy the microorganisms were seen to be located intracellular. The microorganism had a double outer membrane, an oval nucleus, and a kinetoplast out of which (in the phlebotomus fly or in the culture) the future flagellum would be formed.", "PMID": 962386}
{"id": "pubmed23n0032_5008", "title": "Ultrastructural studies in epidermolysis bullosa hereditaria. III. Recessive dystrophic types with dermolytic blistering (Hallopeau-Siemens types and inverse type).", "content": "Ultrastructural examination was performed in 42 biopsy specimens from 22 patients with the Hallopeau-Siemens types or with the inverse type of epidermolysis bullosa dystrophica recessiva. The patient group consists of 8 cases of the localized Hallopeua-Siemens type, 9 of the generalized Hallopeau-Siemens type and 5 of the inverse type. The origins of the biopsy specimens are involved, intact and experimentally frictioned skin from blister-predilected sites, as well as clinically normal skin from nonpredilection sites. It is confirmed that all the blisters initiate below the basal lamina. Anchoring fibrils are moderately to markedly decreased in most cases, while they are normal in 3 other cases. It is thought that secondary degradation of anchoring fibrils and/or collagen fibrils plays an important role in blistering mechanism in the Hallopeau-Siemens and inverse types of recessive dystrophic epidermolysis bullosa, whereas a primary aplasia of anchoring fibrils as causative defect has been out ruled.", "contents": "Ultrastructural studies in epidermolysis bullosa hereditaria. III. Recessive dystrophic types with dermolytic blistering (Hallopeau-Siemens types and inverse type). Ultrastructural examination was performed in 42 biopsy specimens from 22 patients with the Hallopeau-Siemens types or with the inverse type of epidermolysis bullosa dystrophica recessiva. The patient group consists of 8 cases of the localized Hallopeua-Siemens type, 9 of the generalized Hallopeau-Siemens type and 5 of the inverse type. The origins of the biopsy specimens are involved, intact and experimentally frictioned skin from blister-predilected sites, as well as clinically normal skin from nonpredilection sites. It is confirmed that all the blisters initiate below the basal lamina. Anchoring fibrils are moderately to markedly decreased in most cases, while they are normal in 3 other cases. It is thought that secondary degradation of anchoring fibrils and/or collagen fibrils plays an important role in blistering mechanism in the Hallopeau-Siemens and inverse types of recessive dystrophic epidermolysis bullosa, whereas a primary aplasia of anchoring fibrils as causative defect has been out ruled.", "PMID": 962387}
{"id": "pubmed23n0032_5009", "title": "Early metastases in regional lymph nodes and prognosis of malignant melanoma. Histological and clinical examinations in 104 lymphadenectomized patients.", "content": "In 104 malignant melanoma patients who underwent lymphadenectomy (67 females, 37 males), correlations were studied between histologically diagnosed lymph node metastasis, the type of malignant melanoma and the depth of invasion according to Clark, as well as other parameters. In 35.6% of the patients, metastases of the primary tumor were found in one or several regional lymph nodes. In about one third of the patients, the clinical and histological lymph node findings were proven to diverge. The female:male ratio of generally about 2:1 shifted to 1:1 in the group of patients with lymph node metastasis, i.e. cases with lymph node metastasis were found significantly increased in the male sex, and also, when primary tumors were localized on the trunk. A prognostic correlation between the two parameters, sex and localization, is suggested by the high incidence of histologicallly diagnosed metastases in 1 or 2 lymph node regions, when malignant melanomas were localized on the trunk in males. As to the types and the micro-stages of primary tumors, the number of cases collected until now does not permit establishing clear correlations with the incidence of lymph node involvement. Calculating the 5-year-survival rates for patients with and without lymph node metastasis according to the \"actuarial method\", we found the prognosis to depend largely on the presence or absence of lymph node involvement, even at a time as early as at primary tumor excision. Our results support the indication for prophylactic lymphadenectomy in malignant melanoma, provided the primary tumor has reached or surpassed the micro-stage 3.", "contents": "Early metastases in regional lymph nodes and prognosis of malignant melanoma. Histological and clinical examinations in 104 lymphadenectomized patients. In 104 malignant melanoma patients who underwent lymphadenectomy (67 females, 37 males), correlations were studied between histologically diagnosed lymph node metastasis, the type of malignant melanoma and the depth of invasion according to Clark, as well as other parameters. In 35.6% of the patients, metastases of the primary tumor were found in one or several regional lymph nodes. In about one third of the patients, the clinical and histological lymph node findings were proven to diverge. The female:male ratio of generally about 2:1 shifted to 1:1 in the group of patients with lymph node metastasis, i.e. cases with lymph node metastasis were found significantly increased in the male sex, and also, when primary tumors were localized on the trunk. A prognostic correlation between the two parameters, sex and localization, is suggested by the high incidence of histologicallly diagnosed metastases in 1 or 2 lymph node regions, when malignant melanomas were localized on the trunk in males. As to the types and the micro-stages of primary tumors, the number of cases collected until now does not permit establishing clear correlations with the incidence of lymph node involvement. Calculating the 5-year-survival rates for patients with and without lymph node metastasis according to the \"actuarial method\", we found the prognosis to depend largely on the presence or absence of lymph node involvement, even at a time as early as at primary tumor excision. Our results support the indication for prophylactic lymphadenectomy in malignant melanoma, provided the primary tumor has reached or surpassed the micro-stage 3.", "PMID": 962388}
{"id": "pubmed23n0032_5010", "title": "Fluorescence-microscopic investigations of pigment cells of lentigo maligna (melanosis circumscripta praeblastomatosa Dubreuilh) and lentigo maligna melanoma.", "content": "With fluorescence-histochemical methods (formalin-induced fluorescence), the different stadia of development of lentigo maligna (Morbus Dubreuih) and lentigo maligna melanoma were investigated. In this way, the special stadia can be clearly characterized using the fluorescence-microscope. In the earliest stadium of malignancy, only the pigment-cells of the basal part of the epidermis seem to be numerous. At this time, these cells are mainly arranged as palisades in the basal layer, and their strongest dendrites are usually directed towards the corneal layer. As the malignancy progresses, the pigment-cells are arranged in several layers in the basal epidermis. In this case, polymorphism of the cells is obvious and their dendrites spread in all directions. A further stadium shows pathological alterations resembling a lentigo with long rete ridges. The atypical cells cluster together into the so-called pseudonests, predominantly at the tips of these rete ridges. With the fluorescence-microscope, dendrites are seldom visible here. In the stadium of tumorous growth, all above-described alterations of the epidermis in lentigo maligna are no longer detectable. Malignant cells in the dermis, which form as a tumor mode, seldom show dendrites. These cells are mainly round or oval-shaped; at best there are some spindle cells in certain areas of the tumor. The epidermis covering the tumor node is infiltrated by some tumor cells but the characteristic alterations as described above for the various stadia of lentigo maligna are no longer visible. Even fluorescence-microscopically, a tumor node of lentigo maligna does not seem to be different from a primary nodular melanoma or a tumor node of a superficial spreading melanoma, if the flat parts of the tumors are not considered in the diagnosis.", "contents": "Fluorescence-microscopic investigations of pigment cells of lentigo maligna (melanosis circumscripta praeblastomatosa Dubreuilh) and lentigo maligna melanoma. With fluorescence-histochemical methods (formalin-induced fluorescence), the different stadia of development of lentigo maligna (Morbus Dubreuih) and lentigo maligna melanoma were investigated. In this way, the special stadia can be clearly characterized using the fluorescence-microscope. In the earliest stadium of malignancy, only the pigment-cells of the basal part of the epidermis seem to be numerous. At this time, these cells are mainly arranged as palisades in the basal layer, and their strongest dendrites are usually directed towards the corneal layer. As the malignancy progresses, the pigment-cells are arranged in several layers in the basal epidermis. In this case, polymorphism of the cells is obvious and their dendrites spread in all directions. A further stadium shows pathological alterations resembling a lentigo with long rete ridges. The atypical cells cluster together into the so-called pseudonests, predominantly at the tips of these rete ridges. With the fluorescence-microscope, dendrites are seldom visible here. In the stadium of tumorous growth, all above-described alterations of the epidermis in lentigo maligna are no longer detectable. Malignant cells in the dermis, which form as a tumor mode, seldom show dendrites. These cells are mainly round or oval-shaped; at best there are some spindle cells in certain areas of the tumor. The epidermis covering the tumor node is infiltrated by some tumor cells but the characteristic alterations as described above for the various stadia of lentigo maligna are no longer visible. Even fluorescence-microscopically, a tumor node of lentigo maligna does not seem to be different from a primary nodular melanoma or a tumor node of a superficial spreading melanoma, if the flat parts of the tumors are not considered in the diagnosis.", "PMID": 962389}
{"id": "pubmed23n0032_5011", "title": "Comparison of the surface glycoprotein components in the isolated cells of hamster melanotic and amelanotic melanomas.", "content": "A suspension of melanoma cells isolated non-enzymatically from tumors (melanotic and amelanotic) of transplantable melanoma in Syrian hamster was treated with trypsin to obtain surface material. In the material released from the cell surface contents of protein, aminosugars, fucose, sialic acids and hexoses were determined. Differences in the composition of the surface material derived from two kinds of melanoma were observed. The differences in the surface glycoprotein composition seem to be related to biological properties of both melanomas.", "contents": "Comparison of the surface glycoprotein components in the isolated cells of hamster melanotic and amelanotic melanomas. A suspension of melanoma cells isolated non-enzymatically from tumors (melanotic and amelanotic) of transplantable melanoma in Syrian hamster was treated with trypsin to obtain surface material. In the material released from the cell surface contents of protein, aminosugars, fucose, sialic acids and hexoses were determined. Differences in the composition of the surface material derived from two kinds of melanoma were observed. The differences in the surface glycoprotein composition seem to be related to biological properties of both melanomas.", "PMID": 962390}
{"id": "pubmed23n0032_5012", "title": "Which breast to biopsy: an expanding dilemma.", "content": "To provide insight into the significance of findings reported from screening asymptomatic women for breast cancer, we reviewed 19,928 mammographic studies with the accompanying physical examination and correlated these findings with 554 breast biopsies. Of 83 breast biopsies with suspicious findings on both physical examination and mammography, 72 demonstrated breast cancer (87%). Of 155 biopsies for suspicious  changes on mammography alone, 50 (32%) demonstrated breast cancer. The accuracy of suspicious mammography was independent of findings limited to physical examination, 34 (17%) had breast cancer; 31 were in 152 biopsies of patients with mammography interpreted as normal (20%) and three were in biopsies of 52 patients (6%) in whom a visualized  mass was interpreted as benign. One hundred and twelve breast biopsies were performed for changes interpreted as normal or benign. Six malignancies were discovered (5%). No cancer was found in 31 biopsies for nonpalpable benign mammographic abnormalities. Our results emphasize the importance of discriminating between nonvisualization of a mass and mammographic recognition of either a benign or malignant tumor. The reliability of interpretation is considerably greater for a visualized lesion than a nonvisualized one.", "contents": "Which breast to biopsy: an expanding dilemma. To provide insight into the significance of findings reported from screening asymptomatic women for breast cancer, we reviewed 19,928 mammographic studies with the accompanying physical examination and correlated these findings with 554 breast biopsies. Of 83 breast biopsies with suspicious findings on both physical examination and mammography, 72 demonstrated breast cancer (87%). Of 155 biopsies for suspicious  changes on mammography alone, 50 (32%) demonstrated breast cancer. The accuracy of suspicious mammography was independent of findings limited to physical examination, 34 (17%) had breast cancer; 31 were in 152 biopsies of patients with mammography interpreted as normal (20%) and three were in biopsies of 52 patients (6%) in whom a visualized  mass was interpreted as benign. One hundred and twelve breast biopsies were performed for changes interpreted as normal or benign. Six malignancies were discovered (5%). No cancer was found in 31 biopsies for nonpalpable benign mammographic abnormalities. Our results emphasize the importance of discriminating between nonvisualization of a mass and mammographic recognition of either a benign or malignant tumor. The reliability of interpretation is considerably greater for a visualized lesion than a nonvisualized one.", "PMID": 962393}
{"id": "pubmed23n0032_5013", "title": "Immunobiology of operable breast cancer: an assessment of biologic risk by immunoparameters.", "content": "The concept of whether immune function was related to risk of  recurrence was examined in patients with operable breast cancer in whom careful clinical and pathologic staging had been performed. Patients were classified according to the risk of recurrence. The \"low risk\" group included patients with minimal breast cancer, noninfiltrating cancer, or infiltrating cancer less than 1 cm with negative nodes. The \"high ridk\" group included patients with lesions greater than 1 cm or who had greater than or equal to 4 nodal metastases or who had macrometastases at Level II or III (apex). In the \"intermediate risk\" group were patients with infiltrating cancer less than 1 cm or with less than 4 nodal metastases at I only. Immune reactivity was assessed by skin tests, by measurement of absolute lymphocyte count, T and B cells, lymphocyte stimulation by mitogens and a battery of common antigens, serum immunoglobulins and complement levels. There were 134 patients with operable breast cancer and 63 patients with benign breast lesions. The breast cancer patients showed minimal or no impairment of DNCB skin test. Only patients with nodal metastases showed a slight but not significant impairment of DNCB responses (80% were DNCB positive compared to 90% in the controls.) The lymphocyte responses to mitogens were normal in the breast cancer patients, but there was a significant depression of lymphocyte responses to certain recall antigens such as Candida albicans and E coli. The absolute lymphocyte count and the T cell counts were normal, but B cells bearing complement receptors were decreased and B cells bearing sufface immunoglobulins were increased in the breast cancer patients. Analysis of immune function according to the pathologic stage of disease \"risk of recurrence\" categories showed no correlation with skin tests or lymphocyte levels. A striking and paradoxical finding was the demonstration that patients with  \"low risk\" cancer overall had markedly lower responses to the battery of stimulating mitogens and antigens than found in patients with \"high risk\" or \"intermediate risk\" disease. Only the lymphocyte responses to PHA showed a significant linear correlation with increasing pathologic stage or \"risk of recurrence.\" Current evidence from this study suggests that PHA response is markedly influenced by the primary tumor burdenand thus indirectly reflects the risk of recurrence.", "contents": "Immunobiology of operable breast cancer: an assessment of biologic risk by immunoparameters. The concept of whether immune function was related to risk of  recurrence was examined in patients with operable breast cancer in whom careful clinical and pathologic staging had been performed. Patients were classified according to the risk of recurrence. The \"low risk\" group included patients with minimal breast cancer, noninfiltrating cancer, or infiltrating cancer less than 1 cm with negative nodes. The \"high ridk\" group included patients with lesions greater than 1 cm or who had greater than or equal to 4 nodal metastases or who had macrometastases at Level II or III (apex). In the \"intermediate risk\" group were patients with infiltrating cancer less than 1 cm or with less than 4 nodal metastases at I only. Immune reactivity was assessed by skin tests, by measurement of absolute lymphocyte count, T and B cells, lymphocyte stimulation by mitogens and a battery of common antigens, serum immunoglobulins and complement levels. There were 134 patients with operable breast cancer and 63 patients with benign breast lesions. The breast cancer patients showed minimal or no impairment of DNCB skin test. Only patients with nodal metastases showed a slight but not significant impairment of DNCB responses (80% were DNCB positive compared to 90% in the controls.) The lymphocyte responses to mitogens were normal in the breast cancer patients, but there was a significant depression of lymphocyte responses to certain recall antigens such as Candida albicans and E coli. The absolute lymphocyte count and the T cell counts were normal, but B cells bearing complement receptors were decreased and B cells bearing sufface immunoglobulins were increased in the breast cancer patients. Analysis of immune function according to the pathologic stage of disease \"risk of recurrence\" categories showed no correlation with skin tests or lymphocyte levels. A striking and paradoxical finding was the demonstration that patients with  \"low risk\" cancer overall had markedly lower responses to the battery of stimulating mitogens and antigens than found in patients with \"high risk\" or \"intermediate risk\" disease. Only the lymphocyte responses to PHA showed a significant linear correlation with increasing pathologic stage or \"risk of recurrence.\" Current evidence from this study suggests that PHA response is markedly influenced by the primary tumor burdenand thus indirectly reflects the risk of recurrence.", "PMID": 962394}
{"id": "pubmed23n0032_5014", "title": "Limb salvage from a multidisciplinary treatment approach for skeletal and soft tissue sarcomas of the extremity.", "content": "Multimodality management of extremity skeletal and soft tissue sarcomas with preoperative intra-arterial Adriamycin and radiation therapy, radical surgical resection and postoperative chemotherapy or chemo-immunotherapy has resulted in preservation of a functional extremity in 13 or 14 patients. Seven of 8 patients with Stage IIIA and IIIB soft tissue sarcomas, managed with preoperative intra-arterial Adriamycin and radiation therapy, followed by en bloc soft tissue resection and 6 patients with bone sarcomas managed by preoperative treatment, followed by bone resection and replacement with cadaver bone allografts, remained free of disease from 4 to 34 months. The results of the combined modality approach were significantly better than the results obtained in patients managed by surgical resection alone, or by combination of operation with another single modality, both in terms of short term-recurrence free survival and salvage of a  functional extremity.", "contents": "Limb salvage from a multidisciplinary treatment approach for skeletal and soft tissue sarcomas of the extremity. Multimodality management of extremity skeletal and soft tissue sarcomas with preoperative intra-arterial Adriamycin and radiation therapy, radical surgical resection and postoperative chemotherapy or chemo-immunotherapy has resulted in preservation of a functional extremity in 13 or 14 patients. Seven of 8 patients with Stage IIIA and IIIB soft tissue sarcomas, managed with preoperative intra-arterial Adriamycin and radiation therapy, followed by en bloc soft tissue resection and 6 patients with bone sarcomas managed by preoperative treatment, followed by bone resection and replacement with cadaver bone allografts, remained free of disease from 4 to 34 months. The results of the combined modality approach were significantly better than the results obtained in patients managed by surgical resection alone, or by combination of operation with another single modality, both in terms of short term-recurrence free survival and salvage of a  functional extremity.", "PMID": 962395}
{"id": "pubmed23n0032_5015", "title": "Risks in therapeutic portacaval and splenorenal shunts.", "content": "Analyses of the records of 120 patients who underwent portacaval shunting (PCS, 57%) or splenorenal shunting (SRS, 43%) from 1966-1973 disclosed that patients in each group undergoing elective shunts had the same preoperative physical condition and postoperative mortality rates (approximately 20%). Although the post-operative death rate from emergency shunts was 48%, patients having these procedures were poorer risks. Long-term incidences of encephalopathy were the same, irrespective of the type of shunt (PCS, 46%; SRS 36%, P greater than 0.5). Despite comparisons of data most unfavorable for PCS, 5-year survival rates were also the same after either type of shunt (all PCS, 29 +/- 7.5%, SRS, 42.0 +/- 7.4%, P = 0.23). The survival rate after elective PCS was also the same as after SRS during the entire 5-year period. However, the survival after all elective PCS and SRS was significantly greater than after emergency PCS (P range = 0.005-0.038); the poorer results of emergency shunting could be partly attributed to the poorer condition of patients selected. A numerical score based on serum bilirubin concentrations, ascites, and urgency of shunting reliably predicts postoperative mortality. Long-term encephalopathy is predicted by a history of encephalopathy and the urgency of shunting.", "contents": "Risks in therapeutic portacaval and splenorenal shunts. Analyses of the records of 120 patients who underwent portacaval shunting (PCS, 57%) or splenorenal shunting (SRS, 43%) from 1966-1973 disclosed that patients in each group undergoing elective shunts had the same preoperative physical condition and postoperative mortality rates (approximately 20%). Although the post-operative death rate from emergency shunts was 48%, patients having these procedures were poorer risks. Long-term incidences of encephalopathy were the same, irrespective of the type of shunt (PCS, 46%; SRS 36%, P greater than 0.5). Despite comparisons of data most unfavorable for PCS, 5-year survival rates were also the same after either type of shunt (all PCS, 29 +/- 7.5%, SRS, 42.0 +/- 7.4%, P = 0.23). The survival rate after elective PCS was also the same as after SRS during the entire 5-year period. However, the survival after all elective PCS and SRS was significantly greater than after emergency PCS (P range = 0.005-0.038); the poorer results of emergency shunting could be partly attributed to the poorer condition of patients selected. A numerical score based on serum bilirubin concentrations, ascites, and urgency of shunting reliably predicts postoperative mortality. Long-term encephalopathy is predicted by a history of encephalopathy and the urgency of shunting.", "PMID": 962396}
{"id": "pubmed23n0032_5016", "title": "Hepatic artery flow improvement after portacaval shunt: a single hemodynamic clinical correlate.", "content": "We have documented a highly significant increment in hepatic arterial flow following a portacaval shunt in patients with cirrhosis of the liver and portal hypertension. In contrast with other hemodynamic variables, the increment in arterial flow was directly related to morbidity, hospital mortality, and long term survival. Patients with increments smaller than 100 ml/min had the worst clinical results. They accounted for all of the hospital mortality, the largest incidence of encephalopathy, and the worst long term cumulative survival rates. The extent of the increment was not related directly to the type of shunt but, rather, to some intrinsic capability of the cirrhotic liver to increase its arterial flow in response to the relief of sinusoidal hypertension produced by the shunt. This capablilty appears related to the degree of entrapment of the hepatic arterioles by the fibrous tissues of cirrhosis. This encasement of arterioles should change the elastic properties of the hepatic arterial bed and we propose to measure these properties by determining the characteristic input impedance of the arterial bed.", "contents": "Hepatic artery flow improvement after portacaval shunt: a single hemodynamic clinical correlate. We have documented a highly significant increment in hepatic arterial flow following a portacaval shunt in patients with cirrhosis of the liver and portal hypertension. In contrast with other hemodynamic variables, the increment in arterial flow was directly related to morbidity, hospital mortality, and long term survival. Patients with increments smaller than 100 ml/min had the worst clinical results. They accounted for all of the hospital mortality, the largest incidence of encephalopathy, and the worst long term cumulative survival rates. The extent of the increment was not related directly to the type of shunt but, rather, to some intrinsic capability of the cirrhotic liver to increase its arterial flow in response to the relief of sinusoidal hypertension produced by the shunt. This capablilty appears related to the degree of entrapment of the hepatic arterioles by the fibrous tissues of cirrhosis. This encasement of arterioles should change the elastic properties of the hepatic arterial bed and we propose to measure these properties by determining the characteristic input impedance of the arterial bed.", "PMID": 962397}
{"id": "pubmed23n0032_5017", "title": "The surgical treatment of constrictive fibrous endocarditis.", "content": "Constrictive fibrous endocarditis is a pathological entity described by Lo\u00ebffler in 1936. Its etiology is unknown. The clinical course is characterized by an evolution towards cardiac insufficiency leading rapidly to a fatal outcome. Moderen paraclinical investigations are necessary to assess the diagnostic. Caridac catheterization brings the proof of adiastole and angiogardiography reveals the shape of amputation of the ventricle with auriculoventricular regurgitation. The operative procedure consists of resection of the ventricular fibrosis including the valves and auriculo-ventricular valve replacement by a prosthetic valve. The disease affects both Caucasians and Negros. Our experience includes 5 cases. The indications for operation and their results are discussed.", "contents": "The surgical treatment of constrictive fibrous endocarditis. Constrictive fibrous endocarditis is a pathological entity described by Lo\u00ebffler in 1936. Its etiology is unknown. The clinical course is characterized by an evolution towards cardiac insufficiency leading rapidly to a fatal outcome. Moderen paraclinical investigations are necessary to assess the diagnostic. Caridac catheterization brings the proof of adiastole and angiogardiography reveals the shape of amputation of the ventricle with auriculoventricular regurgitation. The operative procedure consists of resection of the ventricular fibrosis including the valves and auriculo-ventricular valve replacement by a prosthetic valve. The disease affects both Caucasians and Negros. Our experience includes 5 cases. The indications for operation and their results are discussed.", "PMID": 962398}
{"id": "pubmed23n0032_5018", "title": "The treatment of acute traumatic rupture of the aorta: a 10-year experience.", "content": "Forty-three patients with aortic rupture secondardy to blunt trauma have been treated at the University of Micigan within the past 10 years with an overall salvage rate of 70%. The diagnosis should be suspected in anyone who has sustained a high speed decelerating injury, if the chest roentgenogram shows media-stinal widening, whether or not there is hypertension of the upper extremities; systolic murmur, or external evidence of chest injury. Aortography should be employed to confirm the diagnosis and to determine the site or sites of rupture. Repair of the lesion should be undertaken as soon as possible and takes priority in most instances over associated injuries. Repair in almost all cases can be accomplished safely and quickly using a bypass shunt without the aid of extracorporeal circulation.", "contents": "The treatment of acute traumatic rupture of the aorta: a 10-year experience. Forty-three patients with aortic rupture secondardy to blunt trauma have been treated at the University of Micigan within the past 10 years with an overall salvage rate of 70%. The diagnosis should be suspected in anyone who has sustained a high speed decelerating injury, if the chest roentgenogram shows media-stinal widening, whether or not there is hypertension of the upper extremities; systolic murmur, or external evidence of chest injury. Aortography should be employed to confirm the diagnosis and to determine the site or sites of rupture. Repair of the lesion should be undertaken as soon as possible and takes priority in most instances over associated injuries. Repair in almost all cases can be accomplished safely and quickly using a bypass shunt without the aid of extracorporeal circulation.", "PMID": 962399}
{"id": "pubmed23n0032_5019", "title": "Long-term survival after resection for bronchogenic carcinoma.", "content": "Of 915 resections for bronchogenic carcinoma over a 25-year period (1945-1969), 249 patients survived over 5 years; 127 of the patients eligible survived over 10 years, 61 over 15 years, and 22 over 20 years. The case material was divided into three time periods: 1945-49, 1950-59 and 1960-69, as well as by extent of resection. Lobectomy became the operation of choice, pneumonectomy being reserved for the more extensive lesions. Observed survival rates  at 5, 10 and 15 years for 561 patients in the lobetomy series were 35, 22 and 15%, respectively, but strikingly increased to 41, 28 and 19% in the 1960-69 period. Observed rates  for 354 patients having pneumonectomies were similar for three time periods, being 16, 8 and 6% at 5, 10 and 15 years, respectively. Relative survival rates for the lobectomy series at 5, 10 and 15 years rose from 33, 28 and 26%, repectively, in the 1950-59 period to 50, 39 and 35% in the last  time period, becoming a near horizontal curve segment after 5 years. Dominant factors in survival were extent of the lesion and stage of nodal involvement, histologic type and location being less significant.", "contents": "Long-term survival after resection for bronchogenic carcinoma. Of 915 resections for bronchogenic carcinoma over a 25-year period (1945-1969), 249 patients survived over 5 years; 127 of the patients eligible survived over 10 years, 61 over 15 years, and 22 over 20 years. The case material was divided into three time periods: 1945-49, 1950-59 and 1960-69, as well as by extent of resection. Lobectomy became the operation of choice, pneumonectomy being reserved for the more extensive lesions. Observed survival rates  at 5, 10 and 15 years for 561 patients in the lobetomy series were 35, 22 and 15%, respectively, but strikingly increased to 41, 28 and 19% in the 1960-69 period. Observed rates  for 354 patients having pneumonectomies were similar for three time periods, being 16, 8 and 6% at 5, 10 and 15 years, respectively. Relative survival rates for the lobectomy series at 5, 10 and 15 years rose from 33, 28 and 26%, repectively, in the 1950-59 period to 50, 39 and 35% in the last  time period, becoming a near horizontal curve segment after 5 years. Dominant factors in survival were extent of the lesion and stage of nodal involvement, histologic type and location being less significant.", "PMID": 962400}
{"id": "pubmed23n0032_5020", "title": "Biliary lipid excretion after hepatic portoenterostomy.", "content": "Since 1974, 16 consecutive infants with biliary atresia have been treated by hepatic portoenterostomy employing an exteriorized Roux-en-Y intestinal segment (Miluliez). Simultaneous, sequential analyses of bile pigments and lipids in serum and biliary drainage were performed. In the 11 patients with sustained bile drainage, progressive increases in bile volume, bilirubin and biliary lipid concentrations correlated well with their subsequent return toward normal in the serum. Despite relief of biliary obstruction, four patients have had progressive liver cirrhosis. The other 7 have residual liver damage which has been stable, or in two instances, improved, at late biopsy. The clinical and biochemical results suggest that both obstructive and parenchymal factors are operative in infants with biliary atresia.", "contents": "Biliary lipid excretion after hepatic portoenterostomy. Since 1974, 16 consecutive infants with biliary atresia have been treated by hepatic portoenterostomy employing an exteriorized Roux-en-Y intestinal segment (Miluliez). Simultaneous, sequential analyses of bile pigments and lipids in serum and biliary drainage were performed. In the 11 patients with sustained bile drainage, progressive increases in bile volume, bilirubin and biliary lipid concentrations correlated well with their subsequent return toward normal in the serum. Despite relief of biliary obstruction, four patients have had progressive liver cirrhosis. The other 7 have residual liver damage which has been stable, or in two instances, improved, at late biopsy. The clinical and biochemical results suggest that both obstructive and parenchymal factors are operative in infants with biliary atresia.", "PMID": 962401}
{"id": "pubmed23n0032_5021", "title": "Repair of ventricular septal defect in the first two years of life using profound hypothermia-circulatory arrest techniques.", "content": "Ventricular septal defect repair had been performed in 57 infants ages 21 days to 21 months and under 10 kg in weight using profound hypothermia-circulatory arrest technics. Severe congestive heart failure was the indication for operation in all but two infants under 6 months of age, and in those under 3 months there was usually an associated moderate or large sized atrial septal defect or patent ductus arteriosus or a coarctation. In infants over 6 months controlled heart failure was accompanied by failure to thrive and often recurrent respiratory infections. The main indication for surgery in three infants was repeated severe respiratory infections and in 7 infants, ages 10-15 months, an elevation of pulmonary vascular resistance of 6 units M2 or more. There were two hospital deaths among the 49 infants without coarctation (ages 6 and 20 months) and two among the 8 with coarctation. Postoperative respiratory and other complications were uncommon. On late review there was no significant residual VSD amongst the 11 recatheterized patients. Psychometric studies in 19  children who had reached the age of three to four years gave no evidence of cerebral damage due to the circulatory arrest period. In view of these results palliative pulmonary artery banding is no longer performed for VSD in infancy unless there is a Swiss cheese septum or an associated severe coarctation.", "contents": "Repair of ventricular septal defect in the first two years of life using profound hypothermia-circulatory arrest techniques. Ventricular septal defect repair had been performed in 57 infants ages 21 days to 21 months and under 10 kg in weight using profound hypothermia-circulatory arrest technics. Severe congestive heart failure was the indication for operation in all but two infants under 6 months of age, and in those under 3 months there was usually an associated moderate or large sized atrial septal defect or patent ductus arteriosus or a coarctation. In infants over 6 months controlled heart failure was accompanied by failure to thrive and often recurrent respiratory infections. The main indication for surgery in three infants was repeated severe respiratory infections and in 7 infants, ages 10-15 months, an elevation of pulmonary vascular resistance of 6 units M2 or more. There were two hospital deaths among the 49 infants without coarctation (ages 6 and 20 months) and two among the 8 with coarctation. Postoperative respiratory and other complications were uncommon. On late review there was no significant residual VSD amongst the 11 recatheterized patients. Psychometric studies in 19  children who had reached the age of three to four years gave no evidence of cerebral damage due to the circulatory arrest period. In view of these results palliative pulmonary artery banding is no longer performed for VSD in infancy unless there is a Swiss cheese septum or an associated severe coarctation.", "PMID": 962402}
{"id": "pubmed23n0032_5022", "title": "Informed consent: recall by patients tested postoperatively.", "content": "When tested for recall between 4 and 6 months following operation, 20 patients failed to remember accurately major portions of their informed consent interview. Documentation of the details of consent in the clinical record is considered advisable.", "contents": "Informed consent: recall by patients tested postoperatively. When tested for recall between 4 and 6 months following operation, 20 patients failed to remember accurately major portions of their informed consent interview. Documentation of the details of consent in the clinical record is considered advisable.", "PMID": 962403}
{"id": "pubmed23n0032_5023", "title": "In vivo evaluation of the Lillehei-Kaster heart valve prosthesis.", "content": "In vivo evaluation of the Lillehei-Kaster heart valve prosthesis was performed in 28 patients; 22 had undergone aortic valve replacement and 6 had had mitral valve replacement. Mean pressure gradients ranged from 6 to 53 mm Hg in aortic prostheses; valve area varied from 0.83 to 2.9 cm2. The maximum opening angle of 80 degrees was never reached in this group of patients; opening angles ranged from 57 to 74 degrees without evidence of disc malfunction. There was no correlation between the ratio of effective to geometric valve area and the degree of opening of the disc occluder. Similar results were found in the mitral valve group. Gradients ranged from 6.0 to 12.5 mm Hg and valve area from 1.6 to 2.2 cm2. The maximum opening angle was 59 to 63 degrees. It is concluded that incomplete opening of the disc occluder in pivoting-disc valves is not necessarily a sign of disc malfunction. Lillehei-Kaster valves smaller than 18 mm in internal diameter should be avoided in adults, and outflow aortoplasty for implantation of a larger prosthesis should be used in patients who have a narrow aortic root.", "contents": "In vivo evaluation of the Lillehei-Kaster heart valve prosthesis. In vivo evaluation of the Lillehei-Kaster heart valve prosthesis was performed in 28 patients; 22 had undergone aortic valve replacement and 6 had had mitral valve replacement. Mean pressure gradients ranged from 6 to 53 mm Hg in aortic prostheses; valve area varied from 0.83 to 2.9 cm2. The maximum opening angle of 80 degrees was never reached in this group of patients; opening angles ranged from 57 to 74 degrees without evidence of disc malfunction. There was no correlation between the ratio of effective to geometric valve area and the degree of opening of the disc occluder. Similar results were found in the mitral valve group. Gradients ranged from 6.0 to 12.5 mm Hg and valve area from 1.6 to 2.2 cm2. The maximum opening angle was 59 to 63 degrees. It is concluded that incomplete opening of the disc occluder in pivoting-disc valves is not necessarily a sign of disc malfunction. Lillehei-Kaster valves smaller than 18 mm in internal diameter should be avoided in adults, and outflow aortoplasty for implantation of a larger prosthesis should be used in patients who have a narrow aortic root.", "PMID": 962404}
{"id": "pubmed23n0032_5024", "title": "Aortic valve replacement with the Hancock porcine xenograft.", "content": "The Hancock porcine xenograft stabilized with the glutaraldehyde process was used for isolated aortic valve replacement in 71 patients from March, 1972, to July, 1975. Aortic stenosis was the primary diagnosis in 52 patients and aortic insufficiency in 19. There were 52 men and 19 women ranging in age from 18 to 82 years; 14 patients were older than 70 years. One patient was in Functional Class II, 50 in Class III, and 20 in Class IV preoperatively. Seventy-three patients undergoing Bj\u00f6rk-Shiley aortic valve replacement during the same period are presented for comparison. The operative mortality was 3% (2 of the 71 patients); 4 of the remaining 69 patients (6%) died in the late postoperative period. No patient was placed on anticoagulation during the postoperative course, and there was 1 postoperative embolus in a patient with chronic atrial fibrillation (1.4%). With a mean follow-up period of 17 months, 60 patients are now in Functional Class I, 4 in Class II, and 1 in Class III. Seven patients have had transvalvular gradients and effective valve areas measured postoperatively that ranged from 32 to 5 mm Hg and 0.9 to 2.9 cm2 in 21 through 25 mm (OD) sizes.", "contents": "Aortic valve replacement with the Hancock porcine xenograft. The Hancock porcine xenograft stabilized with the glutaraldehyde process was used for isolated aortic valve replacement in 71 patients from March, 1972, to July, 1975. Aortic stenosis was the primary diagnosis in 52 patients and aortic insufficiency in 19. There were 52 men and 19 women ranging in age from 18 to 82 years; 14 patients were older than 70 years. One patient was in Functional Class II, 50 in Class III, and 20 in Class IV preoperatively. Seventy-three patients undergoing Bj\u00f6rk-Shiley aortic valve replacement during the same period are presented for comparison. The operative mortality was 3% (2 of the 71 patients); 4 of the remaining 69 patients (6%) died in the late postoperative period. No patient was placed on anticoagulation during the postoperative course, and there was 1 postoperative embolus in a patient with chronic atrial fibrillation (1.4%). With a mean follow-up period of 17 months, 60 patients are now in Functional Class I, 4 in Class II, and 1 in Class III. Seven patients have had transvalvular gradients and effective valve areas measured postoperatively that ranged from 32 to 5 mm Hg and 0.9 to 2.9 cm2 in 21 through 25 mm (OD) sizes.", "PMID": 962405}
{"id": "pubmed23n0032_5025", "title": "Surgical treatment of large ventricular septal defect with pulmonary hypertension in the first 24 months of life.", "content": "A total of 74 patients under 24 months of age with large ventricular septal defects (VSD) and pulmonary hypertension were subjected to surgical treatment from 1969 through 1975. Emergency pulmonary artery (PA) banding was performed in 13 patients during the first year of life with 1 death from postoperative respiratory failure. Primary closure of the VSD was performed in 61 patients using simple hypothermia and short-term coronary perfusion, with an operative mortality of 1.6%. There were no late deaths or neurological disturbances. Normal hemodynamic data were obtained in all 7 patients who underwent postoperative cardiac catheterization from one month to five years after the primary correction. It is concluded that primary closure of a VSD in infancy is reasonable and that PA banding is indicated only for those patients less than 6 months old with a complicated defect or in an emergency situation.", "contents": "Surgical treatment of large ventricular septal defect with pulmonary hypertension in the first 24 months of life. A total of 74 patients under 24 months of age with large ventricular septal defects (VSD) and pulmonary hypertension were subjected to surgical treatment from 1969 through 1975. Emergency pulmonary artery (PA) banding was performed in 13 patients during the first year of life with 1 death from postoperative respiratory failure. Primary closure of the VSD was performed in 61 patients using simple hypothermia and short-term coronary perfusion, with an operative mortality of 1.6%. There were no late deaths or neurological disturbances. Normal hemodynamic data were obtained in all 7 patients who underwent postoperative cardiac catheterization from one month to five years after the primary correction. It is concluded that primary closure of a VSD in infancy is reasonable and that PA banding is indicated only for those patients less than 6 months old with a complicated defect or in an emergency situation.", "PMID": 962406}
{"id": "pubmed23n0032_5026", "title": "The Blalock-Taussig operation: the procedure of choice in the hypoxic infant with tetralogy of Fallot.", "content": "From 1971 to 1975, 17 consecutive patients aged 1 day to 4 years underwent Blalock-Taussig shunts for severe tetralogy of Fallot. Three infants were under 6 weeks of age and 7 (41%) under 1 year. There were no hospital deaths. Modification of the shunt technique adapts it to any size infant. The subclavian artery is divided at its major branches and the end spatulated to enlarge it. The artery is occluded while the shunt is constructed. No intraoperative complications were encountered; all patients have a shunt murmur with no early or late closure. No child has had heart failure or hypoxic spells. Flows measured at operation equaled one-quarter to one-half of the child's normal cardiac output. Ligation of the shunt at subsequent repair is uncomplicated. One child died three years later at correction from causes unrelated to the shunt. With appropriate modifications in technique, the Blalock-Taussig shunt is the operation of choice, at any age, for palliation of severe tetralogy of Fallot.", "contents": "The Blalock-Taussig operation: the procedure of choice in the hypoxic infant with tetralogy of Fallot. From 1971 to 1975, 17 consecutive patients aged 1 day to 4 years underwent Blalock-Taussig shunts for severe tetralogy of Fallot. Three infants were under 6 weeks of age and 7 (41%) under 1 year. There were no hospital deaths. Modification of the shunt technique adapts it to any size infant. The subclavian artery is divided at its major branches and the end spatulated to enlarge it. The artery is occluded while the shunt is constructed. No intraoperative complications were encountered; all patients have a shunt murmur with no early or late closure. No child has had heart failure or hypoxic spells. Flows measured at operation equaled one-quarter to one-half of the child's normal cardiac output. Ligation of the shunt at subsequent repair is uncomplicated. One child died three years later at correction from causes unrelated to the shunt. With appropriate modifications in technique, the Blalock-Taussig shunt is the operation of choice, at any age, for palliation of severe tetralogy of Fallot.", "PMID": 962407}
{"id": "pubmed23n0032_5027", "title": "The operative problem of small left atrium in total anomalous pulmonary venous connection: report of 5 patients.", "content": "A moderately small left atrium is a common finding in total anomalous pulmonary venous connection (TAPVC). In most patients in whom the common pulmonary vein is anastomosed to the left atrium, the small size of this chamber does not interfere with good operative results. Recently a patient was encountered in whom the left atrium measured less than 2 cm in its greatest dimension. This atrium was too small to take an anatomosis with the common pulmonary vein. Therefore the common pulmonary vein was anastomosed primarily to the right atrium, the floor of the fossa ovalis was excised, and a pericardial conduit was constructed. Subsequently, 4 additional patients have been similarly treated. The problem of small left atrium in TAPVC is discussed.", "contents": "The operative problem of small left atrium in total anomalous pulmonary venous connection: report of 5 patients. A moderately small left atrium is a common finding in total anomalous pulmonary venous connection (TAPVC). In most patients in whom the common pulmonary vein is anastomosed to the left atrium, the small size of this chamber does not interfere with good operative results. Recently a patient was encountered in whom the left atrium measured less than 2 cm in its greatest dimension. This atrium was too small to take an anatomosis with the common pulmonary vein. Therefore the common pulmonary vein was anastomosed primarily to the right atrium, the floor of the fossa ovalis was excised, and a pericardial conduit was constructed. Subsequently, 4 additional patients have been similarly treated. The problem of small left atrium in TAPVC is discussed.", "PMID": 962408}
{"id": "pubmed23n0032_5028", "title": "Pulmonary venoocclusive disease.", "content": "Pulmonary venoocclusive disease has been established as a definite clinical entity characterized by congestive cardiac failure with pulmonary arterial hypertension, chronic interstitial pulmonary edema, and normal wedge pressure on cardiac catheterization. This disease was diagnosed and confirmed in a patient during life. A review of the 32 patients reported earlier has been done in an attempt to fine possible etiological agents. Early recognition and treatment with anticoagulants, methylprednisolone, aspirin, and dipyridamole may improve the prognosis.", "contents": "Pulmonary venoocclusive disease. Pulmonary venoocclusive disease has been established as a definite clinical entity characterized by congestive cardiac failure with pulmonary arterial hypertension, chronic interstitial pulmonary edema, and normal wedge pressure on cardiac catheterization. This disease was diagnosed and confirmed in a patient during life. A review of the 32 patients reported earlier has been done in an attempt to fine possible etiological agents. Early recognition and treatment with anticoagulants, methylprednisolone, aspirin, and dipyridamole may improve the prognosis.", "PMID": 962409}
{"id": "pubmed23n0032_5029", "title": "Transaxillary minithoracotomy: the optimal approach for certain pulmonary and mediastinal lesions.", "content": "This report concerns the use of transaxillary thoracotomy for a variety of pulmonary, pleural, and mediastinal conditions in 50 patients. Primary and metastatic carcinomas, pneumothoraces, and benign lesions such as bronchogenic cysts and neurogenic tumors can be identified, evaluated, and treated with confidence. Reduced postoperative pain and morbidity, rapid return of arm and shoulder movements, reduced hospital stay, and excellent cosmetic result are among the advantages of this approach when compared with the usual posterolateral thoracotomy.", "contents": "Transaxillary minithoracotomy: the optimal approach for certain pulmonary and mediastinal lesions. This report concerns the use of transaxillary thoracotomy for a variety of pulmonary, pleural, and mediastinal conditions in 50 patients. Primary and metastatic carcinomas, pneumothoraces, and benign lesions such as bronchogenic cysts and neurogenic tumors can be identified, evaluated, and treated with confidence. Reduced postoperative pain and morbidity, rapid return of arm and shoulder movements, reduced hospital stay, and excellent cosmetic result are among the advantages of this approach when compared with the usual posterolateral thoracotomy.", "PMID": 962410}
{"id": "pubmed23n0032_5030", "title": "Emergency open lung biopsy.", "content": "Twenty-eight patients underwent open lung biopsy on an emergency basis. The clinical course in each case was deteriorating rapidly, and all were in respiratory failure. The correct diagnosis was established in 96% of the biopsies (27 of the 28 patients). The clinical diagnosis prior to biopsy was in error or incomplete in 15 (55%) of the patients. Specific therapy was lifesaving in 12 of the 28 patients. The value of the procedure outweighed the complications. Thrombocytopenia and positive end-expiratory pressure ventilation were not contraindications to biopsy.", "contents": "Emergency open lung biopsy. Twenty-eight patients underwent open lung biopsy on an emergency basis. The clinical course in each case was deteriorating rapidly, and all were in respiratory failure. The correct diagnosis was established in 96% of the biopsies (27 of the 28 patients). The clinical diagnosis prior to biopsy was in error or incomplete in 15 (55%) of the patients. Specific therapy was lifesaving in 12 of the 28 patients. The value of the procedure outweighed the complications. Thrombocytopenia and positive end-expiratory pressure ventilation were not contraindications to biopsy.", "PMID": 962411}
{"id": "pubmed23n0032_5031", "title": "Mediastinopleuroscopy: a new approach to the diagnosis of intrathoracic diseases.", "content": "With the advent of modern therapy, it has become essential to obtain a tissue diagnosis in all cases of pulmonary and mediastinal disease. Since it is often necessary to resort to thoracotomy as a final step in making such a diagnosis, we have sought a procedure that is simpler while capable of providing the same information. Through a standard cervical mediastinoscopy incision, the mediastinum is first explored; if the diagnosis is not obtained, the mediastinal pleura is digitally opened and lung or pleural biopsies are taken. In the course of 1,100 mediastinoscopies since 1969, 275 pleuroscopies have been done. We were able to obtain a tissue diagnosis in 102 (78%) of 131 patients with bronchogenic carcinoma, in 92 (91%) of 102 with benign pulmonary disease, and in all 20 (100%) with pleural disease.", "contents": "Mediastinopleuroscopy: a new approach to the diagnosis of intrathoracic diseases. With the advent of modern therapy, it has become essential to obtain a tissue diagnosis in all cases of pulmonary and mediastinal disease. Since it is often necessary to resort to thoracotomy as a final step in making such a diagnosis, we have sought a procedure that is simpler while capable of providing the same information. Through a standard cervical mediastinoscopy incision, the mediastinum is first explored; if the diagnosis is not obtained, the mediastinal pleura is digitally opened and lung or pleural biopsies are taken. In the course of 1,100 mediastinoscopies since 1969, 275 pleuroscopies have been done. We were able to obtain a tissue diagnosis in 102 (78%) of 131 patients with bronchogenic carcinoma, in 92 (91%) of 102 with benign pulmonary disease, and in all 20 (100%) with pleural disease.", "PMID": 962412}
{"id": "pubmed23n0032_5032", "title": "Carcinoid tumors of the lung.", "content": "Resection of typical bronchial carcinoid was carried out in 203 patients. The average age was 48 years, and the sex distribution was approximately equal. Bronchoscopy was the most definitive diagnostic procedure, even though 15% of the tumors were located in the segmental bronchus or beyond. Conservative resection including local removal of the lesion was the treatment of choice, but distal suppuration and location of the tumor necessitated pneumonectomy in 54 (27%) of the patients. The incidence of metastasis was 5% (11 patients), and the overall hospital mortality was 3%. Of patients who qualified for follow-up, 94% survived 5 years, and of those who were asymptomatic preoperatively, 98% survived 5 years. The 10- and 25-year survival rates for the group as a whole were 87 and 66%, respectively.", "contents": "Carcinoid tumors of the lung. Resection of typical bronchial carcinoid was carried out in 203 patients. The average age was 48 years, and the sex distribution was approximately equal. Bronchoscopy was the most definitive diagnostic procedure, even though 15% of the tumors were located in the segmental bronchus or beyond. Conservative resection including local removal of the lesion was the treatment of choice, but distal suppuration and location of the tumor necessitated pneumonectomy in 54 (27%) of the patients. The incidence of metastasis was 5% (11 patients), and the overall hospital mortality was 3%. Of patients who qualified for follow-up, 94% survived 5 years, and of those who were asymptomatic preoperatively, 98% survived 5 years. The 10- and 25-year survival rates for the group as a whole were 87 and 66%, respectively.", "PMID": 962413}
{"id": "pubmed23n0032_5033", "title": "Reflux control following extended myotomy in primary disordered motor activity (diffuse spasm) of the esophagus.", "content": "We have previously reported the results of extended esophageal myotomy and Belsey hiatal hernia repair in 21 patients. Reflux was considered to be a late complication of this operation, and gastroplasty has subsequently been added. Thirty-four patients have now been surgically treated, 17 with myotomy and Belsey repair and 17 with myotomy, gastroplasty, and Belsey repair. Eight of the 17 with Belsey repair developed clinical and roentgenographic signs of reflux 6 to 27 months following operation without evidence of hernia recurrence; 5 of the 8 patients have required further operation, with the addition of gastroplasty for reflux control. Seventeen patients were treated primarily by extended myotomy, gastroplasty, and Belsey repair. None of the patients who underwent gastroplasty have reflux symptoms, and only 1 shows a trace of reflux radiologically.", "contents": "Reflux control following extended myotomy in primary disordered motor activity (diffuse spasm) of the esophagus. We have previously reported the results of extended esophageal myotomy and Belsey hiatal hernia repair in 21 patients. Reflux was considered to be a late complication of this operation, and gastroplasty has subsequently been added. Thirty-four patients have now been surgically treated, 17 with myotomy and Belsey repair and 17 with myotomy, gastroplasty, and Belsey repair. Eight of the 17 with Belsey repair developed clinical and roentgenographic signs of reflux 6 to 27 months following operation without evidence of hernia recurrence; 5 of the 8 patients have required further operation, with the addition of gastroplasty for reflux control. Seventeen patients were treated primarily by extended myotomy, gastroplasty, and Belsey repair. None of the patients who underwent gastroplasty have reflux symptoms, and only 1 shows a trace of reflux radiologically.", "PMID": 962414}
{"id": "pubmed23n0032_5034", "title": "Simplified lateral chest incision for most thoracotomies other than sternotomy.", "content": "Our experience using the lateral simplified thoracotomy incision for most chest work other than operations requiring median sternotomy is reported. The incision provides adequate exposure, yet preserves major muscle masses and decreases the postoperative morbidity. Return of normal ipsilateral arm function appears to be hastened in the postoperative period. In addition, the incision is easier to open and close than the standard incision.", "contents": "Simplified lateral chest incision for most thoracotomies other than sternotomy. Our experience using the lateral simplified thoracotomy incision for most chest work other than operations requiring median sternotomy is reported. The incision provides adequate exposure, yet preserves major muscle masses and decreases the postoperative morbidity. Return of normal ipsilateral arm function appears to be hastened in the postoperative period. In addition, the incision is easier to open and close than the standard incision.", "PMID": 962415}
{"id": "pubmed23n0032_5035", "title": "Vertical axillary thoracotomy: a functional and cosmetically appealing incision.", "content": "Vertical axillary thoracotomy offers the specific advantages of minimum trauma and maximum preservation of chest wall function. A cosmetically acceptable scar results. The vertical axillary thoracotomy is specifically indicated in patients requiring less than the maximum intrathoracic exposure provided by the most traumatic posterolateral or anterolateral thoracotomy. The surgical technique is presented.", "contents": "Vertical axillary thoracotomy: a functional and cosmetically appealing incision. Vertical axillary thoracotomy offers the specific advantages of minimum trauma and maximum preservation of chest wall function. A cosmetically acceptable scar results. The vertical axillary thoracotomy is specifically indicated in patients requiring less than the maximum intrathoracic exposure provided by the most traumatic posterolateral or anterolateral thoracotomy. The surgical technique is presented.", "PMID": 962416}
{"id": "pubmed23n0032_5036", "title": "Total spinal anesthesia: a rare complication of intrathoracic intercostal nerve block.", "content": "Total spinal anesthesia following intrathoracic intercostal nerve blocks with bupivacaine performed for postoperative pain relief during thoracotomy is described. Possible mechanisms for this complication include: (1) inadvertent placement of the needle through an intervertebral foramen, (2) puncture of a long dural cuff, and (3) intraneural injection with central spread. Recognition of this potential complication is important, and facilities for proper support must be available.", "contents": "Total spinal anesthesia: a rare complication of intrathoracic intercostal nerve block. Total spinal anesthesia following intrathoracic intercostal nerve blocks with bupivacaine performed for postoperative pain relief during thoracotomy is described. Possible mechanisms for this complication include: (1) inadvertent placement of the needle through an intervertebral foramen, (2) puncture of a long dural cuff, and (3) intraneural injection with central spread. Recognition of this potential complication is important, and facilities for proper support must be available.", "PMID": 962417}
{"id": "pubmed23n0032_5037", "title": "Massive pulmonary embolism permitting paradoxical systemic arterial embolism: successful surgical management.", "content": "The case of a young woman, receiving oral contraceptives, who developed massive pulmonary embolism producing circulatory collapse and paradoxical arterial embolism through a patent foramen ovale is documented.. Limb viability was threatened. Emergency management included removal of arterial and pulmonary emboli, surgical closure of the patent foramen ovale, inferior caval partitioning, ovarian vein ligation, and short-term anticoagulation. Recovery was rapid and complete.", "contents": "Massive pulmonary embolism permitting paradoxical systemic arterial embolism: successful surgical management. The case of a young woman, receiving oral contraceptives, who developed massive pulmonary embolism producing circulatory collapse and paradoxical arterial embolism through a patent foramen ovale is documented.. Limb viability was threatened. Emergency management included removal of arterial and pulmonary emboli, surgical closure of the patent foramen ovale, inferior caval partitioning, ovarian vein ligation, and short-term anticoagulation. Recovery was rapid and complete.", "PMID": 962418}
{"id": "pubmed23n0032_5038", "title": "Pulmonary embolism and intracardiac shunt: a unique indication for operation.", "content": "Pulmonary embolism with right-to-left intracardiac shunt presents a special problem for the clinician. A review of the literature and 4 personal cases, all diagnosed antemortem, are presented. On the basis of this information certain conclusions are drawn, and we suggest the problem to be a unique indication for the consideration of surgical intervention.", "contents": "Pulmonary embolism and intracardiac shunt: a unique indication for operation. Pulmonary embolism with right-to-left intracardiac shunt presents a special problem for the clinician. A review of the literature and 4 personal cases, all diagnosed antemortem, are presented. On the basis of this information certain conclusions are drawn, and we suggest the problem to be a unique indication for the consideration of surgical intervention.", "PMID": 962419}
{"id": "pubmed23n0032_5039", "title": "Comparative study of 1-cyclohexyl-4-(1,2-diphenylethyl)-piperazine and its enantiomorphs on analgesic and othe pharmacological activities in experimental animals.", "content": "The analgesic activity of (+/-)-1-cycylohexyl-4-(1,2-diphenylethyl)-piperazine (MT-45) was comparable to that of morphine. The activity of the S(+)-isomer was 1.14 to 1.97 times in mice and 1.00 and 1.23 times in rats as potent as MT-45, and 18.3 to 61.6 times as potent as the R(--)isomer, which was approximately comparable to that of Spa. The hyperglycemic and miotic activities of MT-45 and its S(+)-isomer in rabbits were negligible or very low, though they showed potent morphine-like activites. On the other hand, the R(--)-isomer showed no or very weak effects on those, while Spa showed definite effects. These results suggest that the modes of action of MT-45 and its enantiomorphs are partly different from those of morphine and Spa, and it may be concluded that MT-45 belongs to a new series of compounds having a potent analgesic activity.", "contents": "Comparative study of 1-cyclohexyl-4-(1,2-diphenylethyl)-piperazine and its enantiomorphs on analgesic and othe pharmacological activities in experimental animals. The analgesic activity of (+/-)-1-cycylohexyl-4-(1,2-diphenylethyl)-piperazine (MT-45) was comparable to that of morphine. The activity of the S(+)-isomer was 1.14 to 1.97 times in mice and 1.00 and 1.23 times in rats as potent as MT-45, and 18.3 to 61.6 times as potent as the R(--)isomer, which was approximately comparable to that of Spa. The hyperglycemic and miotic activities of MT-45 and its S(+)-isomer in rabbits were negligible or very low, though they showed potent morphine-like activites. On the other hand, the R(--)-isomer showed no or very weak effects on those, while Spa showed definite effects. These results suggest that the modes of action of MT-45 and its enantiomorphs are partly different from those of morphine and Spa, and it may be concluded that MT-45 belongs to a new series of compounds having a potent analgesic activity.", "PMID": 962421}
{"id": "pubmed23n0032_5040", "title": "The anti-inflammatory profile of proquazone.", "content": "Proquazone, 1-isopropyl-4-phenyl-7-methyl-2(1H)-quinazolinone is an orally effective anti-inflammatory analgesic and antipyretic compound in animal models. The compound is less ulcerogenic than indomethacin in the rat and appears to potentiate the anti-inflammatory and thymus involution effects of hydrocortisone in this species. That a nonacidic nonsteroidal compound would display a spectrum of anti-inflammatory activity similar to that previously found only with acidic compounds will be the subject of further investigations.", "contents": "The anti-inflammatory profile of proquazone. Proquazone, 1-isopropyl-4-phenyl-7-methyl-2(1H)-quinazolinone is an orally effective anti-inflammatory analgesic and antipyretic compound in animal models. The compound is less ulcerogenic than indomethacin in the rat and appears to potentiate the anti-inflammatory and thymus involution effects of hydrocortisone in this species. That a nonacidic nonsteroidal compound would display a spectrum of anti-inflammatory activity similar to that previously found only with acidic compounds will be the subject of further investigations.", "PMID": 962422}
{"id": "pubmed23n0032_5041", "title": "4-nitro-2-phenoxymethanesulfonanilide (R-805): a chemically novel anti-inflammatory agent.", "content": "The anti-inflammatory activity of the sulfonanilide, 4-nitro-2-phenoxymethanesulfonanilide (R-805) has been demonstrated in conventional models (carrageenan-induced edema of the rat's paw, UV-induced erythema of guinea-pig skin, adjuvant-induced arthritis of the rat). R-805 differs from most currently available acidic anti-inflammatory drugs in that its functional acidic group is not carboxyl. The relative anti-inflammatory potency of R-805 is comparable to indomethacin. Calculation of acute therapeutic indices (LD50/ED50) for 8 acidic anti-inflammatory drugs show R-805 to possess a more favourable index than the other drugs examined.", "contents": "4-nitro-2-phenoxymethanesulfonanilide (R-805): a chemically novel anti-inflammatory agent. The anti-inflammatory activity of the sulfonanilide, 4-nitro-2-phenoxymethanesulfonanilide (R-805) has been demonstrated in conventional models (carrageenan-induced edema of the rat's paw, UV-induced erythema of guinea-pig skin, adjuvant-induced arthritis of the rat). R-805 differs from most currently available acidic anti-inflammatory drugs in that its functional acidic group is not carboxyl. The relative anti-inflammatory potency of R-805 is comparable to indomethacin. Calculation of acute therapeutic indices (LD50/ED50) for 8 acidic anti-inflammatory drugs show R-805 to possess a more favourable index than the other drugs examined.", "PMID": 962423}
{"id": "pubmed23n0032_5042", "title": "In-vitro metabolism of etomidate by rat liver fractions.", "content": "(R)-(+)-etomidate and (S)-(-)-etomidate were found to be metabolized in-vitro by various rat liver homogenization fractions: the 16,000 g supernatant fraction caused a more intensive metabolic breakdown than the microsomal fraction; the 100,000 g supernatant fraction was only slightly active. The metabolism was somewhat more rapid and more extensive for the (R)-(+)-etomidate than for the (S)-(-)-isomer. For both isomers, a dose-dependence was observed: the smaller the substrate concentration, the smaller the relative amount of unmetabolized drug, and the more the rate of metabolic breakdown after a certain incubation time slowed down. Only minor qualitative differences between the metabolic pathways of the two isomers were observed. The main metabolic pathway for the in-vitro metabolism was the hydrolysis of the ethyl ester. Decarboxylation and oxidative N-dealkylation were also observed.", "contents": "In-vitro metabolism of etomidate by rat liver fractions. (R)-(+)-etomidate and (S)-(-)-etomidate were found to be metabolized in-vitro by various rat liver homogenization fractions: the 16,000 g supernatant fraction caused a more intensive metabolic breakdown than the microsomal fraction; the 100,000 g supernatant fraction was only slightly active. The metabolism was somewhat more rapid and more extensive for the (R)-(+)-etomidate than for the (S)-(-)-isomer. For both isomers, a dose-dependence was observed: the smaller the substrate concentration, the smaller the relative amount of unmetabolized drug, and the more the rate of metabolic breakdown after a certain incubation time slowed down. Only minor qualitative differences between the metabolic pathways of the two isomers were observed. The main metabolic pathway for the in-vitro metabolism was the hydrolysis of the ethyl ester. Decarboxylation and oxidative N-dealkylation were also observed.", "PMID": 962424}
{"id": "pubmed23n0032_5043", "title": "The plasma protein binding and distribution of etomidate in dog, rat and human blood.", "content": "The interactions of etomidate and its major metabolite  (R 28 141) with plasma proteins were studied by equilibrium dialysis with a multiple cell system. A 4% human serum albumin solution was able to bind 78.5% of the etomidate, and 60.5% of R 25 141, whereas a 1.5% human gamma globulin solution bound etomidate for not more than 3% and did not bind R 28 141 at all. The association constants and free binding energies for the binding of etomidate and R 28 141 to human serum albumin were determined. Plasma protein binding of etomidate was 75.4% in the dog and 76.5% in man; in rat plasma 79.5% of the radioactivity was bound to the plasma proteins, however the etomidate was partly hydrolyzed, even in the presence of sodium fluoride. In the rat 29.7% was distributed to the blood cells, 55.9% bound to plasma proteins and 14.4% was present in plasma water; in the dog the distribution percentages were 42.1%, 43.7% and 14.2% respectively, and in man 37.7%, 47.6% and 14.7% respectively. The major metabolite of etomidate was distributed for 26.3% to the human blood cells, 47.4% was bound to plasma proteins and 26.2% was present in the plasma water; its plasma protein binding amounted to 64.3%. Etomidate was bound at or in the blood cells, whereas R 28 141 was not.", "contents": "The plasma protein binding and distribution of etomidate in dog, rat and human blood. The interactions of etomidate and its major metabolite  (R 28 141) with plasma proteins were studied by equilibrium dialysis with a multiple cell system. A 4% human serum albumin solution was able to bind 78.5% of the etomidate, and 60.5% of R 25 141, whereas a 1.5% human gamma globulin solution bound etomidate for not more than 3% and did not bind R 28 141 at all. The association constants and free binding energies for the binding of etomidate and R 28 141 to human serum albumin were determined. Plasma protein binding of etomidate was 75.4% in the dog and 76.5% in man; in rat plasma 79.5% of the radioactivity was bound to the plasma proteins, however the etomidate was partly hydrolyzed, even in the presence of sodium fluoride. In the rat 29.7% was distributed to the blood cells, 55.9% bound to plasma proteins and 14.4% was present in plasma water; in the dog the distribution percentages were 42.1%, 43.7% and 14.2% respectively, and in man 37.7%, 47.6% and 14.7% respectively. The major metabolite of etomidate was distributed for 26.3% to the human blood cells, 47.4% was bound to plasma proteins and 26.2% was present in the plasma water; its plasma protein binding amounted to 64.3%. Etomidate was bound at or in the blood cells, whereas R 28 141 was not.", "PMID": 962425}
{"id": "pubmed23n0032_5044", "title": "[Comparative activity of ethaverine and papaverine on chronotropic and dromotropic cardiac functions in the anesthetized dog].", "content": "Ethaverine inhibits both atrioventricular conduction and heart rate, starting at a dosage of 1 mg/kg, injected i.v. into the anaesthetized dog. In the experimental conditions used, ethaverine is clearly different from papaverine, which stimulates both at a dosage between 1 and 10 mg/kg, and which only produces an inhibitory effect starting at a dosage of 20 mg/kg, with the appearance of cardiotoxic phenomena. After total cardiac denervation the inhibitory action of ethaverine persists partially, especially at the atrioventricular level, which is probably due to a direct effect on the heart muscle. The observed differences can hardly be explained by a more pronounced cardiac toxicity. Consequently, the ethyl-derivative of papaverine constitutes, in certain aspects, a molecule with original pharmacological properties.", "contents": "[Comparative activity of ethaverine and papaverine on chronotropic and dromotropic cardiac functions in the anesthetized dog]. Ethaverine inhibits both atrioventricular conduction and heart rate, starting at a dosage of 1 mg/kg, injected i.v. into the anaesthetized dog. In the experimental conditions used, ethaverine is clearly different from papaverine, which stimulates both at a dosage between 1 and 10 mg/kg, and which only produces an inhibitory effect starting at a dosage of 20 mg/kg, with the appearance of cardiotoxic phenomena. After total cardiac denervation the inhibitory action of ethaverine persists partially, especially at the atrioventricular level, which is probably due to a direct effect on the heart muscle. The observed differences can hardly be explained by a more pronounced cardiac toxicity. Consequently, the ethyl-derivative of papaverine constitutes, in certain aspects, a molecule with original pharmacological properties.", "PMID": 962426}
{"id": "pubmed23n0032_5045", "title": "Cardiac chronotropic mechanisms of dimethyl sulphoxide: inhibition of acetylcholinesterase and antagonism of negative chronotropy by atropine.", "content": "The chronotropic effects of dimethyl sulphoxide (DMSO) were studied in spontaneously beating rabbit atria. Low concentrations of DMSO (0.14, 0.42 M) produced slight positive chronotropic (+C) responses; 0.84 and 1.41 M DMSO caused significant negative chronotropic (--C) responses. All concentrations decreased contractile strength. Atropine sulphate (10)-7) to 10(-5) M) antagonized the chronotropic effects, but not the inotropic effects. In the presence of atropine all DMSO concentrations produced significant +C responses. Reserpine pretreatment or propranolol did not affect contractile responses to DMSO in the absence or presence of atropine. DMSO produced concentration-dependent inhibition of acetylcholinesterase (AChE) activity of atrial homogenates. The results indicate that the --C responses are due specifically to AChE inhibition by DMSO and resulting cholinergic influences on the atrial pacemaker. Adrenergic mechanisms do not appear to mediate the +C responses. Data presented here provide evidence that a cardioactive effect of DMSO is mediated by a well-defined receptor-linked mechanism, and that this effect can be modified by a specific receptor blocking agent.", "contents": "Cardiac chronotropic mechanisms of dimethyl sulphoxide: inhibition of acetylcholinesterase and antagonism of negative chronotropy by atropine. The chronotropic effects of dimethyl sulphoxide (DMSO) were studied in spontaneously beating rabbit atria. Low concentrations of DMSO (0.14, 0.42 M) produced slight positive chronotropic (+C) responses; 0.84 and 1.41 M DMSO caused significant negative chronotropic (--C) responses. All concentrations decreased contractile strength. Atropine sulphate (10)-7) to 10(-5) M) antagonized the chronotropic effects, but not the inotropic effects. In the presence of atropine all DMSO concentrations produced significant +C responses. Reserpine pretreatment or propranolol did not affect contractile responses to DMSO in the absence or presence of atropine. DMSO produced concentration-dependent inhibition of acetylcholinesterase (AChE) activity of atrial homogenates. The results indicate that the --C responses are due specifically to AChE inhibition by DMSO and resulting cholinergic influences on the atrial pacemaker. Adrenergic mechanisms do not appear to mediate the +C responses. Data presented here provide evidence that a cardioactive effect of DMSO is mediated by a well-defined receptor-linked mechanism, and that this effect can be modified by a specific receptor blocking agent.", "PMID": 962427}
{"id": "pubmed23n0032_5046", "title": "Studies using KWD 2058, a derivative of terbutaline in isolated atrial and tracheal preparations from the guinea-pig.", "content": "KWD 2058 is a derivative of terbutaline in which the ring hydroxy groups are esterified with isobutyric acid. In guinea-pig atrial and tracheal preparations KWD 2058 produced slowly developing beta-receptor mediated actions which were absent in preparations pretreated with physostigmine. Incubation of KWD 2058 with guinea-pig plasma resulted in the production of a compound which was indistinguishable from terbutaline on both a qualitative and quantitative basis. KWD 2058 produced negative inotropic and chronotropic actions and increased the effective refractory period of guinea-pig atria. In addition, KWD 2058 produced a non-competitive antagonism of chronotropic responses to (--)-isoprenaline in atria. It is concluded that KWD 2058 has no affinity for beta-adrenoreceptors and that any beta-agonistic activity displayed is due to its hydrolysis product, terbutaline.", "contents": "Studies using KWD 2058, a derivative of terbutaline in isolated atrial and tracheal preparations from the guinea-pig. KWD 2058 is a derivative of terbutaline in which the ring hydroxy groups are esterified with isobutyric acid. In guinea-pig atrial and tracheal preparations KWD 2058 produced slowly developing beta-receptor mediated actions which were absent in preparations pretreated with physostigmine. Incubation of KWD 2058 with guinea-pig plasma resulted in the production of a compound which was indistinguishable from terbutaline on both a qualitative and quantitative basis. KWD 2058 produced negative inotropic and chronotropic actions and increased the effective refractory period of guinea-pig atria. In addition, KWD 2058 produced a non-competitive antagonism of chronotropic responses to (--)-isoprenaline in atria. It is concluded that KWD 2058 has no affinity for beta-adrenoreceptors and that any beta-agonistic activity displayed is due to its hydrolysis product, terbutaline.", "PMID": 962428}
{"id": "pubmed23n0032_5047", "title": "Effects of dipyridamole on the smooth muscle cells of the guinea-pig's taenia coli.", "content": "Effects of dipyridamole on the smooth muscle of the guinea-pig's taenia coli were studied using the single and double sucrose gap method. Dipyridamole (10(-5) M) affected neither the non-adrenergic inhibitory junction potential, elicited by field stimulation, nor the membrane hyperpolarization induced with ATP applied exogenously, but did potentiate the accompanying relaxations. The muscle spike activity was reduced by dipyridamole without affecting the membrane potential or the membrane resistance. Carbachol induced a depolarization of the muscle cell membrane and thus a contraction of the muscle. Dipyridamole did not modify the membrane potential change produced by carbachol but decreased the size and time course of the contraction. From these results it is concluded that dipyridamole does not potentiate non-adrenergic relaxations by interfering with the neuromuscular transmission, which has been assumed to be purinergic.", "contents": "Effects of dipyridamole on the smooth muscle cells of the guinea-pig's taenia coli. Effects of dipyridamole on the smooth muscle of the guinea-pig's taenia coli were studied using the single and double sucrose gap method. Dipyridamole (10(-5) M) affected neither the non-adrenergic inhibitory junction potential, elicited by field stimulation, nor the membrane hyperpolarization induced with ATP applied exogenously, but did potentiate the accompanying relaxations. The muscle spike activity was reduced by dipyridamole without affecting the membrane potential or the membrane resistance. Carbachol induced a depolarization of the muscle cell membrane and thus a contraction of the muscle. Dipyridamole did not modify the membrane potential change produced by carbachol but decreased the size and time course of the contraction. From these results it is concluded that dipyridamole does not potentiate non-adrenergic relaxations by interfering with the neuromuscular transmission, which has been assumed to be purinergic.", "PMID": 962429}
{"id": "pubmed23n0032_5048", "title": "In vivo antispasmodic activity of Sulcotidil.", "content": "Suloctidil was tested in vivo for its antispasmodic activity on peripheral and cerebral circulations. In the perfused dog hind limb preperation, suloctidil was found to inhibit after 2 and 5 min, the vasospasm induced by norepinephrine or angiotensin; at equal dose, and at the game time intervals, it was more potent than cinnarizine and papaverine. BaCl(2) induced spasms of the pial arterial of the rabbit were also rapidly alleviated by i.a. suloctidil (3.5 mug/kg or more), in conditions where papaverine and vincamine were inactive.", "contents": "In vivo antispasmodic activity of Sulcotidil. Suloctidil was tested in vivo for its antispasmodic activity on peripheral and cerebral circulations. In the perfused dog hind limb preperation, suloctidil was found to inhibit after 2 and 5 min, the vasospasm induced by norepinephrine or angiotensin; at equal dose, and at the game time intervals, it was more potent than cinnarizine and papaverine. BaCl(2) induced spasms of the pial arterial of the rabbit were also rapidly alleviated by i.a. suloctidil (3.5 mug/kg or more), in conditions where papaverine and vincamine were inactive.", "PMID": 962430}
{"id": "pubmed23n0032_5049", "title": "The inhibitory action of dopamine on the anterior tibialis muscle preparation antagonized by pimozide and chlorpromazine.", "content": "In the cat anterior tibialis muscle preparation dopamine produces a decrease on indirectly evoked twitch contraction when it is administered intraarterially but not on intravenous injection. Edrophonium (0.3 mg/kg) exerts only a brief antagonism to the dopamine-induced inhibition. The phenothiazine derivative, chlorpromazine (CPZ, 400 mug/kg) which possesses both alpha and dopaminergic blocking properties significantly reduces the action of dopamine on skeletal muscle. However, phentolamine (1 mg/kg), an alpha adrenergic blocker is without effect, possibly indicating that the dopaminergic blocking characteristic of CPZ is responsible for decreasing the action of dopamine. A more selective dopaminergic blocking agent, pimozide (100 and 200 mug/kg) is an effective antagonist to the paralysis caused by dopamine on the anterior tibialis muscle. The results of this investigation suggests the possibility that a dopaminergic receptor may be present in or on skeletal muscle.", "contents": "The inhibitory action of dopamine on the anterior tibialis muscle preparation antagonized by pimozide and chlorpromazine. In the cat anterior tibialis muscle preparation dopamine produces a decrease on indirectly evoked twitch contraction when it is administered intraarterially but not on intravenous injection. Edrophonium (0.3 mg/kg) exerts only a brief antagonism to the dopamine-induced inhibition. The phenothiazine derivative, chlorpromazine (CPZ, 400 mug/kg) which possesses both alpha and dopaminergic blocking properties significantly reduces the action of dopamine on skeletal muscle. However, phentolamine (1 mg/kg), an alpha adrenergic blocker is without effect, possibly indicating that the dopaminergic blocking characteristic of CPZ is responsible for decreasing the action of dopamine. A more selective dopaminergic blocking agent, pimozide (100 and 200 mug/kg) is an effective antagonist to the paralysis caused by dopamine on the anterior tibialis muscle. The results of this investigation suggests the possibility that a dopaminergic receptor may be present in or on skeletal muscle.", "PMID": 962431}
{"id": "pubmed23n0032_5050", "title": "Factors influencing tolerance to d-amphetamine-induced anorexia in rats.", "content": "Three experiments were concerned with tolerance to anorexia induced by d-amphetamine. In experiment 1, one group of rats on a 2 hr food deprivation schedule received 2 mg/kg of d-amphetamine 15 min before eating every other day for a month. A second group of rats on a similar schedule received the same dose of d-amphetamine immediately after eating. When compared to a saline-treated control group, the former group showed significant decreases in weight and food intake; tolerance to the amphetamine-induced anorexia began to occur toward the end of the experiment. The latter group showed a significant decrease in food intake on the non-drug days and an overall weight loss when compared to the control group. Experiment 2 demonstrated that tolerance to d-amphetamine-anorexia was related to the duration of drug administration per se. Experiment 3 showed that taste can be a factor in influencing the rate of tolerance to d-amphetamine-induced anorexia. These results indicate that both pharmacological and experiential factors play an important role in determining the rate of tolerance to this action of d-amphetamine.", "contents": "Factors influencing tolerance to d-amphetamine-induced anorexia in rats. Three experiments were concerned with tolerance to anorexia induced by d-amphetamine. In experiment 1, one group of rats on a 2 hr food deprivation schedule received 2 mg/kg of d-amphetamine 15 min before eating every other day for a month. A second group of rats on a similar schedule received the same dose of d-amphetamine immediately after eating. When compared to a saline-treated control group, the former group showed significant decreases in weight and food intake; tolerance to the amphetamine-induced anorexia began to occur toward the end of the experiment. The latter group showed a significant decrease in food intake on the non-drug days and an overall weight loss when compared to the control group. Experiment 2 demonstrated that tolerance to d-amphetamine-anorexia was related to the duration of drug administration per se. Experiment 3 showed that taste can be a factor in influencing the rate of tolerance to d-amphetamine-induced anorexia. These results indicate that both pharmacological and experiential factors play an important role in determining the rate of tolerance to this action of d-amphetamine.", "PMID": 962432}
{"id": "pubmed23n0032_5051", "title": "Indirect and direct depressive effects of morphine on activation of lamina V cell of the spinal dorsal horn induced by intra-arterial injection of bradykinin.", "content": "Small doses of morphine (0.3-2.0 mg/kg i.v.) inhibited, in the majority of intact rabbits, the unitary activities of the lumbar lamina V dorsal horn neurons induced by intra-arterial injection of bradykinin, a potent pain producing substance, but not in spinal rabbits. A large dose of morphine (5.0 mg/kg), however, inhibited the bradykinin-induced response even in spinal rabbits. These results suggest that morphine, in small doses, inhibits the sensory transmission of bradykinin-induced pain at the dorsal horn of the spinal cord, through its facilitatory action on the descending inhibitory mechanism; in large doses the level of dorsal horn is directly inhibited.", "contents": "Indirect and direct depressive effects of morphine on activation of lamina V cell of the spinal dorsal horn induced by intra-arterial injection of bradykinin. Small doses of morphine (0.3-2.0 mg/kg i.v.) inhibited, in the majority of intact rabbits, the unitary activities of the lumbar lamina V dorsal horn neurons induced by intra-arterial injection of bradykinin, a potent pain producing substance, but not in spinal rabbits. A large dose of morphine (5.0 mg/kg), however, inhibited the bradykinin-induced response even in spinal rabbits. These results suggest that morphine, in small doses, inhibits the sensory transmission of bradykinin-induced pain at the dorsal horn of the spinal cord, through its facilitatory action on the descending inhibitory mechanism; in large doses the level of dorsal horn is directly inhibited.", "PMID": 962433}
{"id": "pubmed23n0032_5052", "title": "Effects of thyroxine pretreatment and calcium on the isolated perfused rat heart.", "content": "Phosphorylase a activity was the same in isolated perfused hearts from euthyroid and thyroxine-pretreated rats. Perfusion with 3.6 mM Ca2+ caused an increase in phosphorylase a in hearts from euthyroid as well as those from thyroxine-pretreated animals, but the Ca2+-induced stimulation of phosphorylase activity was similar in both groups over the time course studied. Greater conversion of phosphorylase b to a occurred with 7.2 mM than with 3.6 mM Ca2+ in both groups, but once again thyroxine pretreatment did not significantly influence the conversion of phosphorylase b to a. Isometric systolic tension increased in response to 3.6 mM and 7.2 mM Ca2+ in hearts from normal and thyrotoxic rats, but thyroxine pretreatment did not appreciably alter the nature of this response. While spontaneous heart rate was higher in hearts from thyroxine-pretreated rats, perfusion with 3.6 mM or 7.2 mM Ca2+ had no significant effect on heart rate in hearts from euthyroid or thyrotoxic rats.", "contents": "Effects of thyroxine pretreatment and calcium on the isolated perfused rat heart. Phosphorylase a activity was the same in isolated perfused hearts from euthyroid and thyroxine-pretreated rats. Perfusion with 3.6 mM Ca2+ caused an increase in phosphorylase a in hearts from euthyroid as well as those from thyroxine-pretreated animals, but the Ca2+-induced stimulation of phosphorylase activity was similar in both groups over the time course studied. Greater conversion of phosphorylase b to a occurred with 7.2 mM than with 3.6 mM Ca2+ in both groups, but once again thyroxine pretreatment did not significantly influence the conversion of phosphorylase b to a. Isometric systolic tension increased in response to 3.6 mM and 7.2 mM Ca2+ in hearts from normal and thyrotoxic rats, but thyroxine pretreatment did not appreciably alter the nature of this response. While spontaneous heart rate was higher in hearts from thyroxine-pretreated rats, perfusion with 3.6 mM or 7.2 mM Ca2+ had no significant effect on heart rate in hearts from euthyroid or thyrotoxic rats.", "PMID": 962434}
{"id": "pubmed23n0032_5053", "title": "Vas deferens desensitization by noradrenaline and other drugs.", "content": "Desensitization of the isolated rat vas deferens was demonstrated by using noradrenaline, 5-hydroxytryptamine, carbachol, acetylcholine or barium chloride as a test concentration after a large concentration (concentration producing a contraction that was 80% of the maximum) of the same agonist. The large concentration of noradrenaline caused desensitization in response to 5-hydroxytryptamine and acetylcholine but not to carbachol and barium chloride. Desensitization is not altered by reserpine--in pretreatment of rats with reserpine or reserpine in Tyrode solution--imipramine and 6-hydroxydopamine. Surgical denervation or modification of the sodium and calcium concentration of the Tyrode solution did not either alter desensitization. Results show that in the rat isolated vas deferens desensitization is not mediated by an action of noradrenaline on the adrenergic neuron.", "contents": "Vas deferens desensitization by noradrenaline and other drugs. Desensitization of the isolated rat vas deferens was demonstrated by using noradrenaline, 5-hydroxytryptamine, carbachol, acetylcholine or barium chloride as a test concentration after a large concentration (concentration producing a contraction that was 80% of the maximum) of the same agonist. The large concentration of noradrenaline caused desensitization in response to 5-hydroxytryptamine and acetylcholine but not to carbachol and barium chloride. Desensitization is not altered by reserpine--in pretreatment of rats with reserpine or reserpine in Tyrode solution--imipramine and 6-hydroxydopamine. Surgical denervation or modification of the sodium and calcium concentration of the Tyrode solution did not either alter desensitization. Results show that in the rat isolated vas deferens desensitization is not mediated by an action of noradrenaline on the adrenergic neuron.", "PMID": 962435}
{"id": "pubmed23n0032_5054", "title": "Effects of prostaglandins E1, E2 and F2 alpha and polyphloretin phosphate of domestic fowl.", "content": "The predominant effect of PGE1, E2, and F2 alpha on systemic arterial pressure of domestic fowl is hypotension. PGE1 and E2 are approximately equiactive and PGF2 alpha is about 10 times less active than E1 and E2, PGE1 and E2 produce sudden hypotension with gradual recovery while F2 alpha produces fall in blood pressure with both slow onset and recovery. Polyphloretin phosphate itself produces depressor responses in chickens and antagonizes the depressor responses to the prostaglandins dose-dependently.", "contents": "Effects of prostaglandins E1, E2 and F2 alpha and polyphloretin phosphate of domestic fowl. The predominant effect of PGE1, E2, and F2 alpha on systemic arterial pressure of domestic fowl is hypotension. PGE1 and E2 are approximately equiactive and PGF2 alpha is about 10 times less active than E1 and E2, PGE1 and E2 produce sudden hypotension with gradual recovery while F2 alpha produces fall in blood pressure with both slow onset and recovery. Polyphloretin phosphate itself produces depressor responses in chickens and antagonizes the depressor responses to the prostaglandins dose-dependently.", "PMID": 962436}
{"id": "pubmed23n0032_5055", "title": "Duration of analgesia and hyperactivity in mice after single injections (S.C. and I.V.) of heroin.", "content": "ED50 values for analgesia (tail-clip method) at 30 min after heroin HCl administered i.v. or s.c. in groups of Swiss-Webster mice were not significantly different. Nor was the duration of the analgesia following various doses of heroin significantly different after i.v. or s.c. injections, although there was a tendency for the latter route to have a longer effect. Hyperactivity (mean levels in groups of 5 mice, measured by an Activity Meter), though variable, was increased after s.c. injections of heroin in a dose-related manner up to doses of 10 mg/kg. Larger doses progesssively prolonged the peak levels of hyperactivity. Because the degree and duration of both analgesia and hyperactivity were parallel over a wide range of doses of heroin HCl in these mice, a close relationship between the two underlying mechanisms seems very likely.", "contents": "Duration of analgesia and hyperactivity in mice after single injections (S.C. and I.V.) of heroin. ED50 values for analgesia (tail-clip method) at 30 min after heroin HCl administered i.v. or s.c. in groups of Swiss-Webster mice were not significantly different. Nor was the duration of the analgesia following various doses of heroin significantly different after i.v. or s.c. injections, although there was a tendency for the latter route to have a longer effect. Hyperactivity (mean levels in groups of 5 mice, measured by an Activity Meter), though variable, was increased after s.c. injections of heroin in a dose-related manner up to doses of 10 mg/kg. Larger doses progesssively prolonged the peak levels of hyperactivity. Because the degree and duration of both analgesia and hyperactivity were parallel over a wide range of doses of heroin HCl in these mice, a close relationship between the two underlying mechanisms seems very likely.", "PMID": 962437}
{"id": "pubmed23n0032_5056", "title": "The effect of acetylcholine on bile flow.", "content": "The effect of acetylcholine on bile flow was studied in isolated perfused rat liver. Acetylcholine added into the perfusion medium altered bile flow in a way analogous to mechanical clamping and opening of the common bile duct. This effect was potentiated by physostigmine and antagonized by atropine. The results suggest that acetylcholine can pass from portal sinusoidal blood into the biliary tract, possibly through the terminal bars of bile canaliculi, and cause an obstruction in the biliary tree. Suggested mechanism could explain the observed decreased and increased bile flow caused by cholinergic drugs or vagal stimulation in the earlier experiments.", "contents": "The effect of acetylcholine on bile flow. The effect of acetylcholine on bile flow was studied in isolated perfused rat liver. Acetylcholine added into the perfusion medium altered bile flow in a way analogous to mechanical clamping and opening of the common bile duct. This effect was potentiated by physostigmine and antagonized by atropine. The results suggest that acetylcholine can pass from portal sinusoidal blood into the biliary tract, possibly through the terminal bars of bile canaliculi, and cause an obstruction in the biliary tree. Suggested mechanism could explain the observed decreased and increased bile flow caused by cholinergic drugs or vagal stimulation in the earlier experiments.", "PMID": 962438}
{"id": "pubmed23n0032_5057", "title": "Intestinal absorption and biliary excretion kinetics of 14C-labeled methadone in the rat.", "content": "Intestinal abosrption, biliary excretion and enterohepatic circulation of d,l-methadone were studied in male, female, fasted and fed rats by monitoring the appearance of radioactivity in the portal vein, the inferior vena cava, and the bile following intraduodenal administration of 2-14C-d,l-methadone. An early peak in portal concentration was not reflected in the peripheral blood or in the bile. An enterohepatic circulation exists, but is minimal, accounting for less than 1% of the dose in the first hr. Experimental diversion of the bile flow from the lumen of the duodenum has little effect on the relative percentage of methadone vs. metabolites circulating in the blood. However, bile diversion was associated with a 2 to 3 fold increase in the concentration of methadone and its metabolites in portal and peripheral blood 30 min following administration. Eighty to 90% of the 14C in the portal blood is present as methadone and 60 to 70% of the 14C in peripheral blood is methadone while less than 10% of the radioactive materials in the bile is methadone. The amount of metabolite No. 1, metabolite No. 2 and water soluble metabolites vary with over 60% of the radioactive compounds in recirculated bile in the form of water soluble materials.", "contents": "Intestinal absorption and biliary excretion kinetics of 14C-labeled methadone in the rat. Intestinal abosrption, biliary excretion and enterohepatic circulation of d,l-methadone were studied in male, female, fasted and fed rats by monitoring the appearance of radioactivity in the portal vein, the inferior vena cava, and the bile following intraduodenal administration of 2-14C-d,l-methadone. An early peak in portal concentration was not reflected in the peripheral blood or in the bile. An enterohepatic circulation exists, but is minimal, accounting for less than 1% of the dose in the first hr. Experimental diversion of the bile flow from the lumen of the duodenum has little effect on the relative percentage of methadone vs. metabolites circulating in the blood. However, bile diversion was associated with a 2 to 3 fold increase in the concentration of methadone and its metabolites in portal and peripheral blood 30 min following administration. Eighty to 90% of the 14C in the portal blood is present as methadone and 60 to 70% of the 14C in peripheral blood is methadone while less than 10% of the radioactive materials in the bile is methadone. The amount of metabolite No. 1, metabolite No. 2 and water soluble metabolites vary with over 60% of the radioactive compounds in recirculated bile in the form of water soluble materials.", "PMID": 962439}
{"id": "pubmed23n0032_5058", "title": "[The influence on heart conduction time and refractory period of in vitro and in vivo prostaglandins].", "content": "The effects of PGE1, PGE2 and PGF2alpha on conduction time and functional refractory period in the isolated rabbit atrium and in the cat heart in vivo were investigated. In the isolated rabbit atrium the PGs (10(-8)--10(-5) g/ml) were without any effect in this respect. In contrast there was a dose-dependent decrease of conduction time and prolongation of functional refractory period in the cat heart in vivo following PG-infusions (2,0--8,0 mug/kg/min i.v. over a period of 5 min). These findings indicate the absence of a direct quinidine-like membrane action of PGs in the heart. The indirect, depressive effects of PGs on conduction time and functional refractory period in the heart in vivo, should be considered responsible for the antiarrhythmic action of PGs.", "contents": "[The influence on heart conduction time and refractory period of in vitro and in vivo prostaglandins]. The effects of PGE1, PGE2 and PGF2alpha on conduction time and functional refractory period in the isolated rabbit atrium and in the cat heart in vivo were investigated. In the isolated rabbit atrium the PGs (10(-8)--10(-5) g/ml) were without any effect in this respect. In contrast there was a dose-dependent decrease of conduction time and prolongation of functional refractory period in the cat heart in vivo following PG-infusions (2,0--8,0 mug/kg/min i.v. over a period of 5 min). These findings indicate the absence of a direct quinidine-like membrane action of PGs in the heart. The indirect, depressive effects of PGs on conduction time and functional refractory period in the heart in vivo, should be considered responsible for the antiarrhythmic action of PGs.", "PMID": 962440}
{"id": "pubmed23n0032_5059", "title": "Hypertension and renal disease in systemic lupus erythematosus.", "content": "A retrospective analysis of 235 patients at the National Institutes of Health who met at least five criteria for systemic lupus erythematosus (SLE) indicated that 45% were hypertensive. Approximately two thirds of these hypertensive patients had creatinine clearances of more than 60 ml/min and nonnephrotic range proteinuria. Only 16% of normotensive patients had creatinine clearances of less than 60 ml/m9n. A subgroup of 36 patients with SLE and with biopsy-proved diffuse renal disease were studied. For these patients, the presence of hypertension could not be correlated with the degree of proteinuria or hematuria, with the level of serum complement, or with the presence of casts, focal necrosis, crescent formation, or interstitial inflammation. Hypertensive patients had a median age of 24.5 years; the majority had creatinine clearances of more than 60 ml/min. In SLE, hypertension is not necessarily associated with advanced renal disease, and high blood pressure may occur relatively early in the course of the disease.", "contents": "Hypertension and renal disease in systemic lupus erythematosus. A retrospective analysis of 235 patients at the National Institutes of Health who met at least five criteria for systemic lupus erythematosus (SLE) indicated that 45% were hypertensive. Approximately two thirds of these hypertensive patients had creatinine clearances of more than 60 ml/min and nonnephrotic range proteinuria. Only 16% of normotensive patients had creatinine clearances of less than 60 ml/m9n. A subgroup of 36 patients with SLE and with biopsy-proved diffuse renal disease were studied. For these patients, the presence of hypertension could not be correlated with the degree of proteinuria or hematuria, with the level of serum complement, or with the presence of casts, focal necrosis, crescent formation, or interstitial inflammation. Hypertensive patients had a median age of 24.5 years; the majority had creatinine clearances of more than 60 ml/min. In SLE, hypertension is not necessarily associated with advanced renal disease, and high blood pressure may occur relatively early in the course of the disease.", "PMID": 962443}
{"id": "pubmed23n0032_5060", "title": "Secondary pituitary insufficiency: report of three cases of ectopia or hemiagenesis of the thyroid gland.", "content": "Three patients with clinical and laboratory manifestations suggestive of pituitary insufficiency due to the presence of a pituitary tumor were found to have long-standing myxedema. The insidious appearance of the signs of hypothyroidism was explained by the cause of their disease--an ectopic thyroid gland in two patients and hemiagenesis of the thyroid in the third. Early recognition and treatment of such cases is important.", "contents": "Secondary pituitary insufficiency: report of three cases of ectopia or hemiagenesis of the thyroid gland. Three patients with clinical and laboratory manifestations suggestive of pituitary insufficiency due to the presence of a pituitary tumor were found to have long-standing myxedema. The insidious appearance of the signs of hypothyroidism was explained by the cause of their disease--an ectopic thyroid gland in two patients and hemiagenesis of the thyroid in the third. Early recognition and treatment of such cases is important.", "PMID": 962444}
{"id": "pubmed23n0032_5061", "title": "Hemolysis caused by factor VIII concentrates.", "content": "Immune hemolytic anemia is a recognized complication of the use of factor VIII concentrates. Hemolysis is obscured often by the presence of active bleeding. Where hemolysis has been demonstrated, red blood cell (RBC) destruction has been attributed to anti-A antibodies found in the transfused material. We present two episodes of hemolysis associated with the use of factor VIII concentrate. In the first, a high titer of \"immune\" anti-A (1:256) was present in the factor VIII. In the second, the patient's RBCs were group B, and the hemolysis was caused by anti-B antibody in the factor VIII concentrate. In addition, the antibody titer in the material that was received was much lower than previously described. The RBC destruction presumably occurred because of the massive dosage of factor VIII concentrate administered on order to overcome a factor VIII inhibitor.", "contents": "Hemolysis caused by factor VIII concentrates. Immune hemolytic anemia is a recognized complication of the use of factor VIII concentrates. Hemolysis is obscured often by the presence of active bleeding. Where hemolysis has been demonstrated, red blood cell (RBC) destruction has been attributed to anti-A antibodies found in the transfused material. We present two episodes of hemolysis associated with the use of factor VIII concentrate. In the first, a high titer of \"immune\" anti-A (1:256) was present in the factor VIII. In the second, the patient's RBCs were group B, and the hemolysis was caused by anti-B antibody in the factor VIII concentrate. In addition, the antibody titer in the material that was received was much lower than previously described. The RBC destruction presumably occurred because of the massive dosage of factor VIII concentrate administered on order to overcome a factor VIII inhibitor.", "PMID": 962445}
{"id": "pubmed23n0032_5062", "title": "Oral-contraceptive-induced hypertension after adrenalectomy and hypophysectomy.", "content": "The cause of oral-contraceptive-induced hypertension in certain susceptible subjects is obscure. We describe a woman who was receiving replacement doses of fludrocortisone acetate after adrenalectomy and hypophysectomy who developed high blood pressure while ingesting an estrogen-containing oral contraceptive. Renin substrate level was increased, but renin activity was suppressed and unresponsive to tilting. The patient developed a markedly exaggerated natriuresis when infused with saline. Both blood pressure and her responses to infused sodium and tilting normalized after discontinuance of the oral contraceptive medication. In this adrenalectomized patient, the estrogenic component of the pill acting synergistically with a fixed (\"nonsuppressible\") replacement dose of mineralocorticoid seems to have caused a volume-related hypertension.", "contents": "Oral-contraceptive-induced hypertension after adrenalectomy and hypophysectomy. The cause of oral-contraceptive-induced hypertension in certain susceptible subjects is obscure. We describe a woman who was receiving replacement doses of fludrocortisone acetate after adrenalectomy and hypophysectomy who developed high blood pressure while ingesting an estrogen-containing oral contraceptive. Renin substrate level was increased, but renin activity was suppressed and unresponsive to tilting. The patient developed a markedly exaggerated natriuresis when infused with saline. Both blood pressure and her responses to infused sodium and tilting normalized after discontinuance of the oral contraceptive medication. In this adrenalectomized patient, the estrogenic component of the pill acting synergistically with a fixed (\"nonsuppressible\") replacement dose of mineralocorticoid seems to have caused a volume-related hypertension.", "PMID": 962446}
{"id": "pubmed23n0032_5063", "title": "Chiasmal syndrome due to intrasellar abscess.", "content": "A 43-year-old woman had diabetes insipidus and amenorrhea. There was panhypopituitarism on endocrine testing and visual field examination showed inexorably progressive loss to bitemporal hemianopsia. All radiographic findings were normal, but craniotomy disclosed the cause of this chiasmal syndrome to be an intrasellar abscess which, on culture, grew a Gram-positive anaerobe, Peptococcus. Intrasellar or pituitary abscess is rare, but it must always be considered in the differential diagnosis of the chiasmal syndrome, since loss of pituitary and visual function may occur much more rapidly than with the tumors most often responsible for this syndrome and since surgical cure is possible.", "contents": "Chiasmal syndrome due to intrasellar abscess. A 43-year-old woman had diabetes insipidus and amenorrhea. There was panhypopituitarism on endocrine testing and visual field examination showed inexorably progressive loss to bitemporal hemianopsia. All radiographic findings were normal, but craniotomy disclosed the cause of this chiasmal syndrome to be an intrasellar abscess which, on culture, grew a Gram-positive anaerobe, Peptococcus. Intrasellar or pituitary abscess is rare, but it must always be considered in the differential diagnosis of the chiasmal syndrome, since loss of pituitary and visual function may occur much more rapidly than with the tumors most often responsible for this syndrome and since surgical cure is possible.", "PMID": 962447}
{"id": "pubmed23n0032_5064", "title": "Ascaris lumbricoides--a reminder.", "content": "A Vietnamese refugee was admitted to the hospital with signs and symptoms of an acute condition within the abdomen. Examination ultimately led to the roentgenographic diagnosis of an Ascaris lumbricoides infestation. The patient recovered after medical treatment with intravenous fluids and nasogastric suction, followed by piperazine citrate administration. This ubiquitous parasite has the potential for causing many serious abdominal complications. An awareness of this disease is particularly timely, in view of the recent influx of nearly 150,000 Indo-Chinese into North America.", "contents": "Ascaris lumbricoides--a reminder. A Vietnamese refugee was admitted to the hospital with signs and symptoms of an acute condition within the abdomen. Examination ultimately led to the roentgenographic diagnosis of an Ascaris lumbricoides infestation. The patient recovered after medical treatment with intravenous fluids and nasogastric suction, followed by piperazine citrate administration. This ubiquitous parasite has the potential for causing many serious abdominal complications. An awareness of this disease is particularly timely, in view of the recent influx of nearly 150,000 Indo-Chinese into North America.", "PMID": 962448}
{"id": "pubmed23n0032_5065", "title": "Ankylosing spondylitis with complete heart block.", "content": "A patient with complete heart block due to ankylosing spondylitis exhibited an unusually brisk increase in ventricular rate with exercise. Despite broad ventricular (QRS) complexes, His bundle electrograms demonstrated a His deflection before each QRS complex and thus established the level of atrioventricular (A-V) block to be proximal to or localized to the His bundle. A junctional pacemaker accounted for the brisk response to exercise, atropine, or isoproteronol. Left bundle branch block pattern and prolongation of infranodal conduction time (H-Q) suggested additional disease involving the His bundle and/or right fascicle. Wide-spread disease affecting A-V nodal and infranodal conduction was compatible with pathologic changes seen in this disease. Because the pacemaker was able to respond to stress promptly and with appropriate increase in heart rate, the patient appeared not to warrant pacemaker implantation in his present state.", "contents": "Ankylosing spondylitis with complete heart block. A patient with complete heart block due to ankylosing spondylitis exhibited an unusually brisk increase in ventricular rate with exercise. Despite broad ventricular (QRS) complexes, His bundle electrograms demonstrated a His deflection before each QRS complex and thus established the level of atrioventricular (A-V) block to be proximal to or localized to the His bundle. A junctional pacemaker accounted for the brisk response to exercise, atropine, or isoproteronol. Left bundle branch block pattern and prolongation of infranodal conduction time (H-Q) suggested additional disease involving the His bundle and/or right fascicle. Wide-spread disease affecting A-V nodal and infranodal conduction was compatible with pathologic changes seen in this disease. Because the pacemaker was able to respond to stress promptly and with appropriate increase in heart rate, the patient appeared not to warrant pacemaker implantation in his present state.", "PMID": 962449}
{"id": "pubmed23n0032_5066", "title": "Abnormalities of urinary sediment and renal failure following sulfinpyrazone therapy.", "content": "A patient with recurrent thrompophlebitis had sterile pyuria with white blood cell casts and transient, mild renal failure during therapy with sulfinpyrazone. These abnormalities resolved on cessation of therapy, but similar changes in the urinary sediment recurred during therapy with sulfinpyrazone. These abnormalities resolved on cessation of therapy, but similar changes in the urinary sediment recurred during a second course of sulfinpyrazone therapy.", "contents": "Abnormalities of urinary sediment and renal failure following sulfinpyrazone therapy. A patient with recurrent thrompophlebitis had sterile pyuria with white blood cell casts and transient, mild renal failure during therapy with sulfinpyrazone. These abnormalities resolved on cessation of therapy, but similar changes in the urinary sediment recurred during therapy with sulfinpyrazone. These abnormalities resolved on cessation of therapy, but similar changes in the urinary sediment recurred during a second course of sulfinpyrazone therapy.", "PMID": 962450}
{"id": "pubmed23n0032_5067", "title": "Early care of acute myocardial infarction.", "content": "A fixed life support station (LSS) was established in the emergency department of a community hospital in order to provide early care for patients with suspected acute myocardial infarction (MI). Prospective studies were conducted on 154 patients with verified acute MI. Median time from onset of symptoms to electrocardiographic monitoring was 164 minutes. Overall hospital mortality for these patients was 15.6%. Of 112 patients less than 70 years old, 51 arrived within two hours; only three (6%) of the 51 died. Patients arriving within two hours of the onset of symptoms in clinical class I had an incidence of cardiogenic shock (CS) of 2%, while those arriving two hours or more after the onset of symptoms in clinical class II had an incidence of CS of 26% (P less than .005). A fixed LSS in a community hospital is feasible and effective for early care of patients with acute MI and may facilitate identification of patients at highest risk for development of CS.", "contents": "Early care of acute myocardial infarction. A fixed life support station (LSS) was established in the emergency department of a community hospital in order to provide early care for patients with suspected acute myocardial infarction (MI). Prospective studies were conducted on 154 patients with verified acute MI. Median time from onset of symptoms to electrocardiographic monitoring was 164 minutes. Overall hospital mortality for these patients was 15.6%. Of 112 patients less than 70 years old, 51 arrived within two hours; only three (6%) of the 51 died. Patients arriving within two hours of the onset of symptoms in clinical class I had an incidence of cardiogenic shock (CS) of 2%, while those arriving two hours or more after the onset of symptoms in clinical class II had an incidence of CS of 26% (P less than .005). A fixed LSS in a community hospital is feasible and effective for early care of patients with acute MI and may facilitate identification of patients at highest risk for development of CS.", "PMID": 962455}
{"id": "pubmed23n0032_5068", "title": "Reversal of intractable uremic pericarditis by triamcinolone hexacetonide.", "content": "Of 74 patients undergoing long-term hemodialysis who were observed during a 21-month period, seven developed uremic pericarditis. Five of these patients developed intractable pericarditis, unresponsive to intensive dialysis and pericardiocentesis, and were treated with prolonged pericardial drainage (16 to 60 hours) by an indwelling polyethylene catheter and instillation of triamcinolone hexacetonide. Evidence of intractability was based on either the recurrence of cardiac tamponade after pericardiocentesis (two patients) or progression in the size of the pericardial effusion despite four weeks of intensive dialysis (three patients). These five patients recovered and subsequently were observed from 1 to 15 months with no evidence of recurrent pericarditis. This procedure may be an effective alternative to the surgical management of intractable uremic pericarditis, particularly in the patient at high risk for anesthesia and major surgery.", "contents": "Reversal of intractable uremic pericarditis by triamcinolone hexacetonide. Of 74 patients undergoing long-term hemodialysis who were observed during a 21-month period, seven developed uremic pericarditis. Five of these patients developed intractable pericarditis, unresponsive to intensive dialysis and pericardiocentesis, and were treated with prolonged pericardial drainage (16 to 60 hours) by an indwelling polyethylene catheter and instillation of triamcinolone hexacetonide. Evidence of intractability was based on either the recurrence of cardiac tamponade after pericardiocentesis (two patients) or progression in the size of the pericardial effusion despite four weeks of intensive dialysis (three patients). These five patients recovered and subsequently were observed from 1 to 15 months with no evidence of recurrent pericarditis. This procedure may be an effective alternative to the surgical management of intractable uremic pericarditis, particularly in the patient at high risk for anesthesia and major surgery.", "PMID": 962456}
{"id": "pubmed23n0032_5069", "title": "Low-dosage heparin in rapidly progressive glomerulonephritis.", "content": "Two patients developed acute renal failure; creatinine clearances fell to 13 and 34 ml/min, respectively, and one patient was oliguric. Renal biopsies in both patients gave results that were compatible with rapidly progressive glomerulonephritis (RPGN). Both patients were treated with low-dosage heparin sodium infusion (8,000 units/day) and prednisone for two to four weeks, followed by oral anticoagulant (warfarin) and antithrombotic agents (dipyridamole). In the two patients, creatinine clearance rose to at least 60 ml/min, and no bleeding complications were observed. Repeat renal biopsy specimens that were obtained after three to six months of treatment showed no evidence of active glomerulonephritis in either patient, but there was extensive scarring and fibrosis. Low-dosage heparin infusion may arrest and partially reverse the renal failure associated with RPGN in some cases, while avoiding the bleeding complications that are frequently observed in patients treated with larger dosages of heparin.", "contents": "Low-dosage heparin in rapidly progressive glomerulonephritis. Two patients developed acute renal failure; creatinine clearances fell to 13 and 34 ml/min, respectively, and one patient was oliguric. Renal biopsies in both patients gave results that were compatible with rapidly progressive glomerulonephritis (RPGN). Both patients were treated with low-dosage heparin sodium infusion (8,000 units/day) and prednisone for two to four weeks, followed by oral anticoagulant (warfarin) and antithrombotic agents (dipyridamole). In the two patients, creatinine clearance rose to at least 60 ml/min, and no bleeding complications were observed. Repeat renal biopsy specimens that were obtained after three to six months of treatment showed no evidence of active glomerulonephritis in either patient, but there was extensive scarring and fibrosis. Low-dosage heparin infusion may arrest and partially reverse the renal failure associated with RPGN in some cases, while avoiding the bleeding complications that are frequently observed in patients treated with larger dosages of heparin.", "PMID": 962457}
{"id": "pubmed23n0032_5070", "title": "[Drug deaths].", "content": "In the Federal Republic of Germany there exist no statistical datas concerning death related to drug dependence until now. We propose to define death in connexion with drug dependence as the death of a drug dependent person caused by direct or indirect effect of the drug used. We first report on several international investigations; then the results of our own investigation in 40 cases of death in connexion with drug dependence in the district of Nordbaden are presented in detail. As a result it seems necessary to register drug dependent persons and to isolate those who are resistent to any therapy and thus represent a focus for further expansion of drug consuming.", "contents": "[Drug deaths]. In the Federal Republic of Germany there exist no statistical datas concerning death related to drug dependence until now. We propose to define death in connexion with drug dependence as the death of a drug dependent person caused by direct or indirect effect of the drug used. We first report on several international investigations; then the results of our own investigation in 40 cases of death in connexion with drug dependence in the district of Nordbaden are presented in detail. As a result it seems necessary to register drug dependent persons and to isolate those who are resistent to any therapy and thus represent a focus for further expansion of drug consuming.", "PMID": 962461}
{"id": "pubmed23n0032_5071", "title": "[White blood stains?].", "content": "Following to bullet wounds of the upper maxillary region and of the lungs great quantities of blood were expectorated by the victim soiling the clothes of the perpetrator. (These cloth consisted of a mixture of wool and synthetic fibers producing a rough surface.) The suit was washed out in cold water and dried. The only macroscopic findings were intensively stained white to grey spots missing any brown or red color portion. These were identified to consist of fibrin. Extensive examination proved the existence of blood spots of group A. Experimental stains applied to the same cloth showed identical changes after washing out.", "contents": "[White blood stains?]. Following to bullet wounds of the upper maxillary region and of the lungs great quantities of blood were expectorated by the victim soiling the clothes of the perpetrator. (These cloth consisted of a mixture of wool and synthetic fibers producing a rough surface.) The suit was washed out in cold water and dried. The only macroscopic findings were intensively stained white to grey spots missing any brown or red color portion. These were identified to consist of fibrin. Extensive examination proved the existence of blood spots of group A. Experimental stains applied to the same cloth showed identical changes after washing out.", "PMID": 962462}
{"id": "pubmed23n0032_5072", "title": "Cysteine and S-sulfocysteine biosynthesis in phototrophic bacteria.", "content": "Forteen species (17 strains) of phototrophic bacteria as well as one strain of Thiobacillus denitrificans were tested for cysteine synthase and S-sulfocysteine synthase. All strains contain cysteine sythase active with O-acetylserine; only the Chromatiaceae, two species of the Rhodospirillaceae and T. denitrificans contain S-sulfocysteine synthase. In six species repression by different sulfur compounds in the medium was studied. In Chromatium vinosum, cysteine synthase was found to be constitutive, while in the Rhodospirillaceae tested the enzyme is repressed by sulfide. Thiosulfate had a derepressive effect in Rhodopseudomonas globiformis but strongly repressed cysteine synthase in R. sulfidophila and R. palustris. Cysteine had only moderate effects with the species tested.", "contents": "Cysteine and S-sulfocysteine biosynthesis in phototrophic bacteria. Forteen species (17 strains) of phototrophic bacteria as well as one strain of Thiobacillus denitrificans were tested for cysteine synthase and S-sulfocysteine synthase. All strains contain cysteine sythase active with O-acetylserine; only the Chromatiaceae, two species of the Rhodospirillaceae and T. denitrificans contain S-sulfocysteine synthase. In six species repression by different sulfur compounds in the medium was studied. In Chromatium vinosum, cysteine synthase was found to be constitutive, while in the Rhodospirillaceae tested the enzyme is repressed by sulfide. Thiosulfate had a derepressive effect in Rhodopseudomonas globiformis but strongly repressed cysteine synthase in R. sulfidophila and R. palustris. Cysteine had only moderate effects with the species tested.", "PMID": 962465}
{"id": "pubmed23n0032_5073", "title": "A description of glucose uptake in Navicula pelliculosa (Breb) Hilse including a brief comparison with an associated Flavobacterium sp.", "content": "Navicula pelliculosa and an associated Flavobacterium sp. were isolated from the epiphyton of Scirpus maritimus, an emergent macrophyte growing in a brackish drainage dyke. Both micro-organisms possessed active transport systems for glucose uptake. In N. pelliculosa the transport system was fully induced in the dark in the absence of glucose, and subsequently inactivated when transferred to the light in the absence of the substrate. The presence of glucose during the dark induction period prevented the achievement of maximum specific activity of the transport system, while incubation at a high light intensity with or without the presence of the substrate resulted in a very marked inhibition of glucose uptake. Inhibition in the light was partially offset by blocking photosynthetic electron flow with 3'(3,4 dichlorophenyl)1'1' dimethyl urea. The transport system accumulated 3-O-methyl glucose against a concentration gradient and was highly specific for glucose as there was no competition by most of the other sugars tested. However, 6-deoxyglucose was taken up instead of glucose and this suggested that glucose was transported in a non-phosphorylated state, whereas inhibition of glucose transport activity with dicyclohexylcarbodimide implicated the involvement of an adenosine triphosphatase on the cell membrane. Inhibitors of oxidative phosphorylation tetrachlorosalicylaniline and carbonylcyanide m-chlorophenylhydrazone also inhibited glucose transport activity. The affinity of the diatom for glucose was greater than that shown by the bacterium, but the Km for glucose transport, 1.5x10-5M was too high to allow effective removal of glucose at in situ concentrations.", "contents": "A description of glucose uptake in Navicula pelliculosa (Breb) Hilse including a brief comparison with an associated Flavobacterium sp. Navicula pelliculosa and an associated Flavobacterium sp. were isolated from the epiphyton of Scirpus maritimus, an emergent macrophyte growing in a brackish drainage dyke. Both micro-organisms possessed active transport systems for glucose uptake. In N. pelliculosa the transport system was fully induced in the dark in the absence of glucose, and subsequently inactivated when transferred to the light in the absence of the substrate. The presence of glucose during the dark induction period prevented the achievement of maximum specific activity of the transport system, while incubation at a high light intensity with or without the presence of the substrate resulted in a very marked inhibition of glucose uptake. Inhibition in the light was partially offset by blocking photosynthetic electron flow with 3'(3,4 dichlorophenyl)1'1' dimethyl urea. The transport system accumulated 3-O-methyl glucose against a concentration gradient and was highly specific for glucose as there was no competition by most of the other sugars tested. However, 6-deoxyglucose was taken up instead of glucose and this suggested that glucose was transported in a non-phosphorylated state, whereas inhibition of glucose transport activity with dicyclohexylcarbodimide implicated the involvement of an adenosine triphosphatase on the cell membrane. Inhibitors of oxidative phosphorylation tetrachlorosalicylaniline and carbonylcyanide m-chlorophenylhydrazone also inhibited glucose transport activity. The affinity of the diatom for glucose was greater than that shown by the bacterium, but the Km for glucose transport, 1.5x10-5M was too high to allow effective removal of glucose at in situ concentrations.", "PMID": 962466}
{"id": "pubmed23n0032_5074", "title": "[Kluyveromyces blattae sp. n., a new multispored yeast for Blatta orientalis (author's transl)].", "content": "A new hitherto undescribed species of yeast of the genus Kluyveromyces has been isolated for the intestinal tract of the oriental cockroach (Blatta orientalis). A description of the new species including latin diagnosis is given.", "contents": "[Kluyveromyces blattae sp. n., a new multispored yeast for Blatta orientalis (author's transl)]. A new hitherto undescribed species of yeast of the genus Kluyveromyces has been isolated for the intestinal tract of the oriental cockroach (Blatta orientalis). A description of the new species including latin diagnosis is given.", "PMID": 962467}
{"id": "pubmed23n0032_5075", "title": "On the effect of glucono-delta-lactone on the yeast Pichia polymorpha.", "content": "Investigation has been made into the action of glucono-delta-lactone on living cells of Pichia polymorpha in relation to the uptake of D-(U-14C)glucose, and the incorporation of (2-14C) uracil and L-(U-14C) threonine into RNA and protein respectively. Other factors such as the action of glucono-delta-lactone on cell morphology and on enzymic synthesis have also been studied. The action of this compound on beta-glucanase has been found to take place in the hydrolytic power and not in the synthesis.", "contents": "On the effect of glucono-delta-lactone on the yeast Pichia polymorpha. Investigation has been made into the action of glucono-delta-lactone on living cells of Pichia polymorpha in relation to the uptake of D-(U-14C)glucose, and the incorporation of (2-14C) uracil and L-(U-14C) threonine into RNA and protein respectively. Other factors such as the action of glucono-delta-lactone on cell morphology and on enzymic synthesis have also been studied. The action of this compound on beta-glucanase has been found to take place in the hydrolytic power and not in the synthesis.", "PMID": 962468}
{"id": "pubmed23n0032_5076", "title": "[Purification and characterization of peroxidase from Phellinus igniarius (author's transl)].", "content": "A Peroxidase (EC 1.11.1.7) of the basidiomycet Phellinus igniarius was derived from mycel and a medium containing glucose and extract of yeast by using various methods of preparation. The enzyme resists extreme conditions (pH, temperature salt concentration). Its optimum pH for activities is in the acid range. Two isoenzymes were found. The molecular weight, isoelectric point, Michaelis-Menten constant, indolacetic acid oxidase activity and spectral and analytical properties of this peroxidase were determined. It is assumed that the enzyme has an intracellular as well as an extracellular field of activity.", "contents": "[Purification and characterization of peroxidase from Phellinus igniarius (author's transl)]. A Peroxidase (EC 1.11.1.7) of the basidiomycet Phellinus igniarius was derived from mycel and a medium containing glucose and extract of yeast by using various methods of preparation. The enzyme resists extreme conditions (pH, temperature salt concentration). Its optimum pH for activities is in the acid range. Two isoenzymes were found. The molecular weight, isoelectric point, Michaelis-Menten constant, indolacetic acid oxidase activity and spectral and analytical properties of this peroxidase were determined. It is assumed that the enzyme has an intracellular as well as an extracellular field of activity.", "PMID": 962469}
{"id": "pubmed23n0032_5077", "title": "Some effects of light on the viability of rhodopsin-containing halobacteria.", "content": "Under starvation conditions, rhodopsin-containing halobacteria show a light-accelerated death under aerobic conditions. This is attributed to photooxidative processes. Under anaerobic conditions, halobacteria die rapidly in the dark, and light prevents death. Since it has been shown by others that lightdriven ATP synthesis can occur under anaerobic conditions, it is postulated that rhodopsin-mediated photophosphorylation is of survival value for this organism in the brines in which it lives, especially because the solubility of oxygen is low in highly saline waters and anaerobic conditions can often develop.", "contents": "Some effects of light on the viability of rhodopsin-containing halobacteria. Under starvation conditions, rhodopsin-containing halobacteria show a light-accelerated death under aerobic conditions. This is attributed to photooxidative processes. Under anaerobic conditions, halobacteria die rapidly in the dark, and light prevents death. Since it has been shown by others that lightdriven ATP synthesis can occur under anaerobic conditions, it is postulated that rhodopsin-mediated photophosphorylation is of survival value for this organism in the brines in which it lives, especially because the solubility of oxygen is low in highly saline waters and anaerobic conditions can often develop.", "PMID": 962470}
{"id": "pubmed23n0032_5078", "title": "Mating in Chlamydomonas eugametos. A scanning electron microscopical study.", "content": "A technique has been developed by which mating gametes of Chlamydomonas eugametos can be studied in the Scanning Electron Microscope. A detailed description of the mating process, from the initial flagellar agglutination until the release of free vis-\u00e0-vis pairs, is presented. Flagella appear to agglutinate at random points on their surface. This is followed by a rapid increase of the contact area such that they \"line-up\" tip to tip. Flagella always exhibit a typical position prior to cell fusion. After cell fusion the flagella of a pair separate rapidly while the female have shortened about 33%. In a vis-\u00e0-vis pair the  plasma bridge has contracted. The observations are interpreted in terms of a specific reorganization of the sexuale aggregate.", "contents": "Mating in Chlamydomonas eugametos. A scanning electron microscopical study. A technique has been developed by which mating gametes of Chlamydomonas eugametos can be studied in the Scanning Electron Microscope. A detailed description of the mating process, from the initial flagellar agglutination until the release of free vis-\u00e0-vis pairs, is presented. Flagella appear to agglutinate at random points on their surface. This is followed by a rapid increase of the contact area such that they \"line-up\" tip to tip. Flagella always exhibit a typical position prior to cell fusion. After cell fusion the flagella of a pair separate rapidly while the female have shortened about 33%. In a vis-\u00e0-vis pair the  plasma bridge has contracted. The observations are interpreted in terms of a specific reorganization of the sexuale aggregate.", "PMID": 962471}
{"id": "pubmed23n0032_5079", "title": "Characteristics of ribonucleic acids isolated for Botryodiplodia theobromae pycnidiospores.", "content": "Nucleic acids isolated for dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two \"ribosomal-associated\" species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two \"ribosomal-associated\" RNA components. Molecular weights of 1.20,0.67,0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In  addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.", "contents": "Characteristics of ribonucleic acids isolated for Botryodiplodia theobromae pycnidiospores. Nucleic acids isolated for dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two \"ribosomal-associated\" species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two \"ribosomal-associated\" RNA components. Molecular weights of 1.20,0.67,0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In  addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.", "PMID": 962472}
{"id": "pubmed23n0032_5080", "title": "Metabiolic products of microorganisms. Tirandamycin B(author's transl).", "content": "Streptomyces flaveolus, strain T\u00fc 1240 produces besides Tirandamycin A, a hitherto unknown antibiotic, which is closely related to Tirandamycin A. The new antibiotic Tirandamycin B contains one additional hydroxylgroup. Both antibiotics exhibit a similar antimicrobial spectrum and they seem to have the same mechanism of action. According to the data obtained from mass spectrometry, 13C-and 1H-NMR spectra formula II could be deduced for Tirandamycin B.", "contents": "Metabiolic products of microorganisms. Tirandamycin B(author's transl). Streptomyces flaveolus, strain T\u00fc 1240 produces besides Tirandamycin A, a hitherto unknown antibiotic, which is closely related to Tirandamycin A. The new antibiotic Tirandamycin B contains one additional hydroxylgroup. Both antibiotics exhibit a similar antimicrobial spectrum and they seem to have the same mechanism of action. According to the data obtained from mass spectrometry, 13C-and 1H-NMR spectra formula II could be deduced for Tirandamycin B.", "PMID": 962473}
{"id": "pubmed23n0032_5081", "title": "[Parenteral administration of sorbitol solution and its impact on sorbitol, glucose, fructose and lactate concentration in the blood of cattle, sheep and piglets].", "content": "Significant rise of fructose in the blood plasma of cattle resulted from an application of sorbite solution (0.5 g sorbite per kg live weight), with its onset as early as during the infusion. The highest concentration was reached 15 minutes after completion of infusion. Rise in glucose (11 mg/100 ml plasma on average) failed to prove statistically significant and dropped temporarily (after 60 to 120 minutes) below the original value. The lactate level in the blood went up temporarily and reached its maximum 30 minutes from the end of infusion. Sorbite solution (1 g/kg live weight) was intravenously applied to sheep and tock fructose to its maximum after 30 minutes. Intraperitoneal application of sorbite solution (1 g/kg live weight) was tolerated well also by piglets and triggered an age-dependent decline of glucose in blood plasma.", "contents": "[Parenteral administration of sorbitol solution and its impact on sorbitol, glucose, fructose and lactate concentration in the blood of cattle, sheep and piglets]. Significant rise of fructose in the blood plasma of cattle resulted from an application of sorbite solution (0.5 g sorbite per kg live weight), with its onset as early as during the infusion. The highest concentration was reached 15 minutes after completion of infusion. Rise in glucose (11 mg/100 ml plasma on average) failed to prove statistically significant and dropped temporarily (after 60 to 120 minutes) below the original value. The lactate level in the blood went up temporarily and reached its maximum 30 minutes from the end of infusion. Sorbite solution (1 g/kg live weight) was intravenously applied to sheep and tock fructose to its maximum after 30 minutes. Intraperitoneal application of sorbite solution (1 g/kg live weight) was tolerated well also by piglets and triggered an age-dependent decline of glucose in blood plasma.", "PMID": 962477}
{"id": "pubmed23n0032_5082", "title": "[Endocrine and spermatogenic testicular function. 2. Correlations between food protein quality, testosterone level in testes, spermatogenesis and nuclear volume of interstitial cells of Leydig in the adult rat].", "content": "Male adult Wistar rats were given different food proteins through 21 days, including casein and four-per-cent methionine (K diet), maize gluten with amino acid added (M + As diet), maize gluten alone (M diet), and casein with four-per-cent methionine mixed one to three with gelatin (K/G diet). Testicular testosterone levels per 100 g live weight were about 411 ng (in animals aged 130 days) or 326 ng in animals aged 160 days) following 21 days of K or M + As feeding (rRP = ten per cent). Roughly 70 per cent of those values were obtained by M or K/G feeding. The use of 20 per cent rRP gave 342 ng in connection with M + As feeding or 289 ng when it accompanied M feeding (the latter figures being recorded from animals aged 160 days). The eight cyclic phases of the germinal epithelium were completely development, independent of RP quality, in all test animals. The nuclear volume of the interstitial cells of Leydig responded with highly significant variation (p less than 0.01) to diets with ten per cent rRP of different qualities, but no significant morphokinetic effect was recorded by karyometric from diets with 20 per cent rRP.", "contents": "[Endocrine and spermatogenic testicular function. 2. Correlations between food protein quality, testosterone level in testes, spermatogenesis and nuclear volume of interstitial cells of Leydig in the adult rat]. Male adult Wistar rats were given different food proteins through 21 days, including casein and four-per-cent methionine (K diet), maize gluten with amino acid added (M + As diet), maize gluten alone (M diet), and casein with four-per-cent methionine mixed one to three with gelatin (K/G diet). Testicular testosterone levels per 100 g live weight were about 411 ng (in animals aged 130 days) or 326 ng in animals aged 160 days) following 21 days of K or M + As feeding (rRP = ten per cent). Roughly 70 per cent of those values were obtained by M or K/G feeding. The use of 20 per cent rRP gave 342 ng in connection with M + As feeding or 289 ng when it accompanied M feeding (the latter figures being recorded from animals aged 160 days). The eight cyclic phases of the germinal epithelium were completely development, independent of RP quality, in all test animals. The nuclear volume of the interstitial cells of Leydig responded with highly significant variation (p less than 0.01) to diets with ten per cent rRP of different qualities, but no significant morphokinetic effect was recorded by karyometric from diets with 20 per cent rRP.", "PMID": 962478}
{"id": "pubmed23n0032_5083", "title": "[Nutrient demand of erysipelothrix bacteria].", "content": "Studies into the nutrient demand of Erysipelothrix insidiosa led to the preparation of a nutritive medium with high bacterial yield which is recommended for quantity breeding of bacteria. The medium was complex and did not contain serum but the following components: peptone S, yeast extract, glucose, Na2HPO4, arginine, and Tween 80.", "contents": "[Nutrient demand of erysipelothrix bacteria]. Studies into the nutrient demand of Erysipelothrix insidiosa led to the preparation of a nutritive medium with high bacterial yield which is recommended for quantity breeding of bacteria. The medium was complex and did not contain serum but the following components: peptone S, yeast extract, glucose, Na2HPO4, arginine, and Tween 80.", "PMID": 962479}
{"id": "pubmed23n0032_5084", "title": "[Variations of glucose, fructose, sorbite and electrolyte concentration following intravenous or intraperitoneal administration of sorbite-electrolyte solution to cattle and sheep].", "content": "Different quantities of sorbite-electrolyte solution were intravenously administered to eight heads of cattle and four heads of sheep (application values being 50 g sorbite, 0.3049 g MgCl2-6H2O, 0.3728 g KCl, 0.5477 g CaCl2-6H2O, 5.265 g NaCl, 6.804 g sodium acetate-3H2O with 1,000 ml distilled water). Different rises of sorbite, fructose, and glucose were recorded from the blood plasma. Certain manifestations of incompatibility and intolerance phenomena were observed, among them increase of cardiorespiratory activity and muscular tremor. Those findings were obtained primarily from animals which exhibited also strong rise in glucose concentration. One of the sheep died. Larger quantities of solution (2,000 ml or 4,000 ml) were intraperitoneally applied to ten heads of cattle and tolerated by them with no reaction. Sorbite in blood plasma usually reached its maximum two or three hours from application, however, without any rise of fructose or glucose. Slow drip infusion or intraperitoneal infusion are the techniques recommended for application of the above sorbite-electrolyte solution to ruminants.", "contents": "[Variations of glucose, fructose, sorbite and electrolyte concentration following intravenous or intraperitoneal administration of sorbite-electrolyte solution to cattle and sheep]. Different quantities of sorbite-electrolyte solution were intravenously administered to eight heads of cattle and four heads of sheep (application values being 50 g sorbite, 0.3049 g MgCl2-6H2O, 0.3728 g KCl, 0.5477 g CaCl2-6H2O, 5.265 g NaCl, 6.804 g sodium acetate-3H2O with 1,000 ml distilled water). Different rises of sorbite, fructose, and glucose were recorded from the blood plasma. Certain manifestations of incompatibility and intolerance phenomena were observed, among them increase of cardiorespiratory activity and muscular tremor. Those findings were obtained primarily from animals which exhibited also strong rise in glucose concentration. One of the sheep died. Larger quantities of solution (2,000 ml or 4,000 ml) were intraperitoneally applied to ten heads of cattle and tolerated by them with no reaction. Sorbite in blood plasma usually reached its maximum two or three hours from application, however, without any rise of fructose or glucose. Slow drip infusion or intraperitoneal infusion are the techniques recommended for application of the above sorbite-electrolyte solution to ruminants.", "PMID": 962480}
{"id": "pubmed23n0032_5085", "title": "Mortality in depressed patients treated with electroconvulsive therapy and antidepressants.", "content": "The treatments of 519 depressed patients hospitalized from 1959 to 1969 were compared in a three-year follow-up study with particular reference to mortality. The electroconvulsive therapy (ECT) group had a significantly lower mortality than the inadequate antidepressant treatment group (P less than .05) and the group that received neither ECT nor antidepressants (P less than .025). Although the adequate antidepressant treatment group had a low mortality, statistically significant differences between this and other treatment groups could not be documented. Nonsuicidal deaths (P less than .005), and particularly myocardial infarctions (P less than .01), were significantly more frequent in the inadequately treated group compared to the adequately treated group. The superiority of adequate treatment is especially striking among men and among the older age groups. The results underscore the importance of adequate treatment of depression, especially in the older man.", "contents": "Mortality in depressed patients treated with electroconvulsive therapy and antidepressants. The treatments of 519 depressed patients hospitalized from 1959 to 1969 were compared in a three-year follow-up study with particular reference to mortality. The electroconvulsive therapy (ECT) group had a significantly lower mortality than the inadequate antidepressant treatment group (P less than .05) and the group that received neither ECT nor antidepressants (P less than .025). Although the adequate antidepressant treatment group had a low mortality, statistically significant differences between this and other treatment groups could not be documented. Nonsuicidal deaths (P less than .005), and particularly myocardial infarctions (P less than .01), were significantly more frequent in the inadequately treated group compared to the adequately treated group. The superiority of adequate treatment is especially striking among men and among the older age groups. The results underscore the importance of adequate treatment of depression, especially in the older man.", "PMID": 962487}
{"id": "pubmed23n0032_5086", "title": "Neuroendocrine regulation in depression. I. Limbic system-adrenocortical dysfunction.", "content": "The regulation of hypothalamopituitary-adrenal (HPA) function in depressed patients was studied by a midnight dexamethasone suppression test. By using an observation period of 24 hours postadministration of dexamethasone, a graded series of abnormal test responses was identified. Depressed patients show abnormal early escape from suppression rather than absolute resistance to HPA suppression by dexamethasone. With increasing severity of depression, this escape occurs progressively more early on the day after administration of dexamethasone. These abnormalities were strongly related to the presence of HPA hyperactivity before dexamethasone was given. The essential disturbance of neuroendocrine regulation in depression is a failure of the normal brain inhibitory influence on the HPA system. This disinhibition of HPA activity suggests that there is an abnormal limbic system drive on the HPA axis in primary depressive illness.", "contents": "Neuroendocrine regulation in depression. I. Limbic system-adrenocortical dysfunction. The regulation of hypothalamopituitary-adrenal (HPA) function in depressed patients was studied by a midnight dexamethasone suppression test. By using an observation period of 24 hours postadministration of dexamethasone, a graded series of abnormal test responses was identified. Depressed patients show abnormal early escape from suppression rather than absolute resistance to HPA suppression by dexamethasone. With increasing severity of depression, this escape occurs progressively more early on the day after administration of dexamethasone. These abnormalities were strongly related to the presence of HPA hyperactivity before dexamethasone was given. The essential disturbance of neuroendocrine regulation in depression is a failure of the normal brain inhibitory influence on the HPA system. This disinhibition of HPA activity suggests that there is an abnormal limbic system drive on the HPA axis in primary depressive illness.", "PMID": 962488}
{"id": "pubmed23n0032_5087", "title": "Neuroendocrine regulation in depression. II. Discrimination of depressed from nondepressed patients.", "content": "Forty-two patients with endogenomorphic depression (ED) and 42 patients with other psychiatric disorders received an overnight dexamethasone test of hypothalamopituitary-adrenal (HPA) suppressibility. Plasma and urinary cortisol measures showed that the ED patients had significantly greater HPA activity before dexamethasone and less complete HPA suppression after dexamethasone. High cortisol vlaues after dexamethasone correlated strongly with spontaneous HPA disinhibition, as indicated by high baseline midnight plasma cortisol levels. Criteria for defining normal suppression responses were developed. All patients with depressive neuroses and most patients with other nondepressive disorders had completely normal responses to dexamethasone. About half of the ED patients had abnormal responses, whether or not they were receiving other drugs at the time of the test. Drug-free patients with depressive neuroses or other disorders showed no abnormal responses to dexamethasone. The effects of psychotropic drugs on the test require further study. Patients with two or more abnormal cortisol values after administration of dexamethasone were identified correctly as ED at confidence levels close to 100%. The dexamethasone suppression test may be of value as a laboratory aid in the diagnosis of \"endogenous\" depression.", "contents": "Neuroendocrine regulation in depression. II. Discrimination of depressed from nondepressed patients. Forty-two patients with endogenomorphic depression (ED) and 42 patients with other psychiatric disorders received an overnight dexamethasone test of hypothalamopituitary-adrenal (HPA) suppressibility. Plasma and urinary cortisol measures showed that the ED patients had significantly greater HPA activity before dexamethasone and less complete HPA suppression after dexamethasone. High cortisol vlaues after dexamethasone correlated strongly with spontaneous HPA disinhibition, as indicated by high baseline midnight plasma cortisol levels. Criteria for defining normal suppression responses were developed. All patients with depressive neuroses and most patients with other nondepressive disorders had completely normal responses to dexamethasone. About half of the ED patients had abnormal responses, whether or not they were receiving other drugs at the time of the test. Drug-free patients with depressive neuroses or other disorders showed no abnormal responses to dexamethasone. The effects of psychotropic drugs on the test require further study. Patients with two or more abnormal cortisol values after administration of dexamethasone were identified correctly as ED at confidence levels close to 100%. The dexamethasone suppression test may be of value as a laboratory aid in the diagnosis of \"endogenous\" depression.", "PMID": 962489}
{"id": "pubmed23n0032_5088", "title": "Incidence and signficiance of mixed affective states in a bipolar population.", "content": "The simultaneous existence of manic and depressive symptoms in a manic-depressive patient was first described by Kraepelin. This so-called mixed state has come to have much theoretical significance in recent hypotheses about the nature of bipolar affective disorders. In this investigation a mixed state is shown to be the initial episode in 31% of 84 manic-depressive outpatients. Moreover, the presence of \"mixed\" features does not correlate with severity of illness or mood circularity, but does correlate with sedative abuse and poor response to psychopharmacologic treatment. These results suggest that the \"continuum hypothesis\" and its satellite theories represent viable conceptualizations of the nature of manic-depressive illness.", "contents": "Incidence and signficiance of mixed affective states in a bipolar population. The simultaneous existence of manic and depressive symptoms in a manic-depressive patient was first described by Kraepelin. This so-called mixed state has come to have much theoretical significance in recent hypotheses about the nature of bipolar affective disorders. In this investigation a mixed state is shown to be the initial episode in 31% of 84 manic-depressive outpatients. Moreover, the presence of \"mixed\" features does not correlate with severity of illness or mood circularity, but does correlate with sedative abuse and poor response to psychopharmacologic treatment. These results suggest that the \"continuum hypothesis\" and its satellite theories represent viable conceptualizations of the nature of manic-depressive illness.", "PMID": 962490}
{"id": "pubmed23n0032_5089", "title": "Hopelessness, depression, and suicide intent.", "content": "One hundred fifty-four suicide attempters, threateners, and psychiatric controls were rated on suicide intent scales and given tests to assess hopelessness, depression and self-rated suicide risk. Ninety-four subjects were retested one month later. Both hopelessness and depression were significantly greater in suicidal subjects. In threatenets, hopelessness and depression scores differed significantly between high and low suicide intent subjects. In attempters ranked by suicide intent at time of testing, more and less suicidal subjects differed significantly in hopelessness and depression scores. Both depression and hopelessness were sensitive to changes in suicide risk during the one-month follow-up. In all analyses, hopelessness correlated more highly with suicide intent than did depression. The data were regarded as supporting the hypothesis that hopelessness is more closely related to suicide intent than is depression.", "contents": "Hopelessness, depression, and suicide intent. One hundred fifty-four suicide attempters, threateners, and psychiatric controls were rated on suicide intent scales and given tests to assess hopelessness, depression and self-rated suicide risk. Ninety-four subjects were retested one month later. Both hopelessness and depression were significantly greater in suicidal subjects. In threatenets, hopelessness and depression scores differed significantly between high and low suicide intent subjects. In attempters ranked by suicide intent at time of testing, more and less suicidal subjects differed significantly in hopelessness and depression scores. Both depression and hopelessness were sensitive to changes in suicide risk during the one-month follow-up. In all analyses, hopelessness correlated more highly with suicide intent than did depression. The data were regarded as supporting the hypothesis that hopelessness is more closely related to suicide intent than is depression.", "PMID": 962491}
{"id": "pubmed23n0032_5090", "title": "Emergence of myasthenia gravis during treatment with lithium carbonate.", "content": "In a patient with recurrent episodes of severe mania, classical manifestations of myasthenia gravis developed for the first time during treatment with lithium carbonate. Four episodes were recorded in which the appearance of progressive weakness coincided with subacute or long-term lithium carbonate administration during periods of psychiatric remission. In each instance, neurologic symptoms were ameliorated or disappeared shortly after the drug was discontinued or its dosage was reduced. There was no evidence of lithium carbonate toxicity or electrolyte disturbances at any time during treatment. Based on a literature review of animal studies and related clinical reports, a mechanism of peripherally mediated neuromuscular cholinergic insufficiency is proposed. In addition, the differential diagnosis of muscle weakness during lithium carbonate administration is discussed.", "contents": "Emergence of myasthenia gravis during treatment with lithium carbonate. In a patient with recurrent episodes of severe mania, classical manifestations of myasthenia gravis developed for the first time during treatment with lithium carbonate. Four episodes were recorded in which the appearance of progressive weakness coincided with subacute or long-term lithium carbonate administration during periods of psychiatric remission. In each instance, neurologic symptoms were ameliorated or disappeared shortly after the drug was discontinued or its dosage was reduced. There was no evidence of lithium carbonate toxicity or electrolyte disturbances at any time during treatment. Based on a literature review of animal studies and related clinical reports, a mechanism of peripherally mediated neuromuscular cholinergic insufficiency is proposed. In addition, the differential diagnosis of muscle weakness during lithium carbonate administration is discussed.", "PMID": 962492}
{"id": "pubmed23n0032_5091", "title": "Correlation between plasma and cerebrospinal levels of imipramine.", "content": "This study measured the levels of imipramine hydrochloride and desipramine hydrochloride (desmethylimipramine) in the plasma and cerebrospinal fluid (CSF) in 11 depressed patients. The oral doses correlated significantly with the plasma levels irrespective of different diagnostic categories. The CSF levels varied significantly. In the endogenous depressive group the CSF levels were significantly higher in responders as compared to nonresponders. The CSF levels of the nonresponders in the endogenous depressive group, and of both responders and nonresponders in the schizo-affective groups, were similar.", "contents": "Correlation between plasma and cerebrospinal levels of imipramine. This study measured the levels of imipramine hydrochloride and desipramine hydrochloride (desmethylimipramine) in the plasma and cerebrospinal fluid (CSF) in 11 depressed patients. The oral doses correlated significantly with the plasma levels irrespective of different diagnostic categories. The CSF levels varied significantly. In the endogenous depressive group the CSF levels were significantly higher in responders as compared to nonresponders. The CSF levels of the nonresponders in the endogenous depressive group, and of both responders and nonresponders in the schizo-affective groups, were similar.", "PMID": 962493}
{"id": "pubmed23n0032_5092", "title": "Assessment of social adjustment by patient self-report.", "content": "Current emphasis on early case finding, outpatient care, and on longitudinal studies of asymptomatic patients has focused attention on the community adjustment of psychiatric patients. Thus, simple and inexpensive methods such as self-report scales, which allow the routine assessment of patient adjustment, are potentially useful. The derivation and testing of such a method, the Social Adjustment Scale Self-Report, is described. This scale covers the patient's role performance, interpersonal relationships, friction, feelings and satisfaction in work, and social and leisure activities with the extended family, as a spouse, parent, and member of a family unit. Self-report results based on 76 depressed outpatients were comparable to those obtained from relatives as well as by a rater who interviewed the patient directly.", "contents": "Assessment of social adjustment by patient self-report. Current emphasis on early case finding, outpatient care, and on longitudinal studies of asymptomatic patients has focused attention on the community adjustment of psychiatric patients. Thus, simple and inexpensive methods such as self-report scales, which allow the routine assessment of patient adjustment, are potentially useful. The derivation and testing of such a method, the Social Adjustment Scale Self-Report, is described. This scale covers the patient's role performance, interpersonal relationships, friction, feelings and satisfaction in work, and social and leisure activities with the extended family, as a spouse, parent, and member of a family unit. Self-report results based on 76 depressed outpatients were comparable to those obtained from relatives as well as by a rater who interviewed the patient directly.", "PMID": 962494}
{"id": "pubmed23n0032_5093", "title": "Personality patterns in insomnia. Theoretical implications.", "content": "Subjects with a primary complaint of insomnia (N = 124) were evaluated with Minnesota Multiphasic Personality Inventories (MMPIs). A high percentage of subjects (85%) had one or more MMPI scales elevated to a pathological degree. The scales most elevated were, in order. 2 (depression), 7 (psychasthenia), and 3 (conversion hysteria). A striking finding was the preponderance of depression. This was indicated by the frequency in which scale 2 was elevated above 70, the frequency in which this this scale had the highest elevation, and the frequency of MMPI code types that included scale 2. Four common MMPI code types representing various types of depression were noted, indicating considerable homogeneity for code types in this sample. The predominant personality styles in this sample were characterized by the internalization of psychological distrubances rather than by acting out or aggression. We propose that this internalization produces a state of constant emotional arousal and resultant physiological activation and that this process is a psychophysiological mechansim underlyling insomnia.", "contents": "Personality patterns in insomnia. Theoretical implications. Subjects with a primary complaint of insomnia (N = 124) were evaluated with Minnesota Multiphasic Personality Inventories (MMPIs). A high percentage of subjects (85%) had one or more MMPI scales elevated to a pathological degree. The scales most elevated were, in order. 2 (depression), 7 (psychasthenia), and 3 (conversion hysteria). A striking finding was the preponderance of depression. This was indicated by the frequency in which scale 2 was elevated above 70, the frequency in which this this scale had the highest elevation, and the frequency of MMPI code types that included scale 2. Four common MMPI code types representing various types of depression were noted, indicating considerable homogeneity for code types in this sample. The predominant personality styles in this sample were characterized by the internalization of psychological distrubances rather than by acting out or aggression. We propose that this internalization produces a state of constant emotional arousal and resultant physiological activation and that this process is a psychophysiological mechansim underlyling insomnia.", "PMID": 962495}
{"id": "pubmed23n0032_5094", "title": "The pro-heroin effects of anti-opium laws in Asia.", "content": "Over 25 years anti-opium laws were enacted by three Asian governments in countries where opium use was traditional. Within months, heroin use suddenly appeared; and within a decade, heroin addiction surpassed opium addiction. The laws led to (1) increased price of narcotic drugs, (2) a heroin \"industry,\" (3) corruption of the law enforcement system, and (4) major health problems involving parenteral drug use. The Asian experience indicates that antinarcotic laws can be effective only with careful preparations: (1) changing society's attitude toward the traditional drug from ambivalence to opposition; (2) mobilizing resources to treat and rehabilitate all addicts within a short period of time; (3) developing the social will to incarcerate all \"recidivist\" addicts for a prolonged period; and (4) preventing narcotic production or importation.", "contents": "The pro-heroin effects of anti-opium laws in Asia. Over 25 years anti-opium laws were enacted by three Asian governments in countries where opium use was traditional. Within months, heroin use suddenly appeared; and within a decade, heroin addiction surpassed opium addiction. The laws led to (1) increased price of narcotic drugs, (2) a heroin \"industry,\" (3) corruption of the law enforcement system, and (4) major health problems involving parenteral drug use. The Asian experience indicates that antinarcotic laws can be effective only with careful preparations: (1) changing society's attitude toward the traditional drug from ambivalence to opposition; (2) mobilizing resources to treat and rehabilitate all addicts within a short period of time; (3) developing the social will to incarcerate all \"recidivist\" addicts for a prolonged period; and (4) preventing narcotic production or importation.", "PMID": 962496}
{"id": "pubmed23n0032_5095", "title": "[On epidemiology of gastric cancer (author's transl)].", "content": "257 stomach cancer patients and 766 controls were interviewed using a standardized questionnaire for 35 variables. The two groups were compared by multivariate variance analysis. This analysis results in a combination of 20 variables significantly connected with gastric cancer. The rankorder of the most prominent variables is as follows: no food intolerance, higher alcohol consumption, living place near Berlin, occupation labourer, early loss of teeth, car driving, no gallstones, smoking and age. It can be assumed that these variables are of aetiological importance. In the aetiology of gastric cancer, exogenous cancerogens seem to play a more important role than endogenous factors. In two further multivariate variance analysis, control group is divided into persons with and persons without chronic gastritis verified by blind gastric biopsy and compared with gastric cancer patients, too. A fourth analysis is limited to female only. The results of the three last mentioned analysis are similar to those of the first one and also similar to results from other countries based on univariate analysis. The computed combination of variables was used for diagnosis of gastric cancer by discriminance analysis in the persons forming the material for this study. This diagnosis was correct in 71% of gastric cancer patients and in 72% of control persons.", "contents": "[On epidemiology of gastric cancer (author's transl)]. 257 stomach cancer patients and 766 controls were interviewed using a standardized questionnaire for 35 variables. The two groups were compared by multivariate variance analysis. This analysis results in a combination of 20 variables significantly connected with gastric cancer. The rankorder of the most prominent variables is as follows: no food intolerance, higher alcohol consumption, living place near Berlin, occupation labourer, early loss of teeth, car driving, no gallstones, smoking and age. It can be assumed that these variables are of aetiological importance. In the aetiology of gastric cancer, exogenous cancerogens seem to play a more important role than endogenous factors. In two further multivariate variance analysis, control group is divided into persons with and persons without chronic gastritis verified by blind gastric biopsy and compared with gastric cancer patients, too. A fourth analysis is limited to female only. The results of the three last mentioned analysis are similar to those of the first one and also similar to results from other countries based on univariate analysis. The computed combination of variables was used for diagnosis of gastric cancer by discriminance analysis in the persons forming the material for this study. This diagnosis was correct in 71% of gastric cancer patients and in 72% of control persons.", "PMID": 962497}
{"id": "pubmed23n0032_5096", "title": "[Malignant neoplasms of the stomach in Berlin, capital of the G.D.R. - An analysis of the state of diagnosis and treatment].", "content": "In Berlin, capital of the German Democratic Republic, 7,386 new cases of gastric cancer (ICD 151) were registered in the period from 1955 to 1969 and in year 1973. 52 percent of patients were male. Percentage of cases 75 years old and more, increased from 21% (1955-1959) to 36% (1965-1969). There were only slight changes in incidence. About 30% of cases were in the operable stages I and II. With rising age, the proportion of the advanced stages III and IV increases. No real progress as made in early diagnosis of stomach cancer as measured by stage distribution during observation period. Patient's delay from first symptom to first visit was shorter than one month in 40%, physician's delay from first visit to correct diagnosis was shorter than one month in 54%. 15% of patients were treated within one month after first symptom and 47% within three months. There was no correlation between duration of history and percentage of early stages. When observation periods 1955 to 1959, 1960-1964, 1965-1969 and 1973 are compared, we find an increase in old patients (75+ years), a decrease of localized stages I + II, and a decrease in resection rate. Therefore, a decrease in survival rates is to be expected. Crude 5-year survival rate was 6.4% (1955-1959) and 6.2% (1960-1964). When we compare data from Berlin with observations in the Region of Erfurt and in Birmingham Region, the situation of detection and treatment of stomach cancer in Berlin seems to be somewhat better. Finally, some suggestions for the improvement of control of stomach cancer are made.", "contents": "[Malignant neoplasms of the stomach in Berlin, capital of the G.D.R. - An analysis of the state of diagnosis and treatment]. In Berlin, capital of the German Democratic Republic, 7,386 new cases of gastric cancer (ICD 151) were registered in the period from 1955 to 1969 and in year 1973. 52 percent of patients were male. Percentage of cases 75 years old and more, increased from 21% (1955-1959) to 36% (1965-1969). There were only slight changes in incidence. About 30% of cases were in the operable stages I and II. With rising age, the proportion of the advanced stages III and IV increases. No real progress as made in early diagnosis of stomach cancer as measured by stage distribution during observation period. Patient's delay from first symptom to first visit was shorter than one month in 40%, physician's delay from first visit to correct diagnosis was shorter than one month in 54%. 15% of patients were treated within one month after first symptom and 47% within three months. There was no correlation between duration of history and percentage of early stages. When observation periods 1955 to 1959, 1960-1964, 1965-1969 and 1973 are compared, we find an increase in old patients (75+ years), a decrease of localized stages I + II, and a decrease in resection rate. Therefore, a decrease in survival rates is to be expected. Crude 5-year survival rate was 6.4% (1955-1959) and 6.2% (1960-1964). When we compare data from Berlin with observations in the Region of Erfurt and in Birmingham Region, the situation of detection and treatment of stomach cancer in Berlin seems to be somewhat better. Finally, some suggestions for the improvement of control of stomach cancer are made.", "PMID": 962498}
{"id": "pubmed23n0032_5097", "title": "[A model for the valuation of mass attendance and attendance duration of patients with malignant neoplasms (author's transpl)].", "content": "By means of a combination of two methods known in demography - life table and estimates for intercensal years - probabilities of prevalence of cancer patients and duration of disease from onset to death are calculated. Starting-points are the incidence know by cancerregistration and death certificates. The results of the model are compared with the results of a special point prevalence counting. Both methods gave about the same results.", "contents": "[A model for the valuation of mass attendance and attendance duration of patients with malignant neoplasms (author's transpl)]. By means of a combination of two methods known in demography - life table and estimates for intercensal years - probabilities of prevalence of cancer patients and duration of disease from onset to death are calculated. Starting-points are the incidence know by cancerregistration and death certificates. The results of the model are compared with the results of a special point prevalence counting. Both methods gave about the same results.", "PMID": 962499}
{"id": "pubmed23n0032_5098", "title": "[Investigations for using cell- and organ culture in sensibility tests for cytostatics in human ovarial-carcinomas (author's transl)].", "content": "Comparison was proved from meaning of cell culture and organ culture in therapeutic sensibility tests for cytostatics in ten human ovarial-carcinomas. The sensibility for cytostatics was determined with morphological criteria in cell culture and with the activity-patterns of some enzymes (lactate-dehydrogenase, NADH-diahorase) in organ culture. In these investigations the tumors have shown approximate conformity in the state to cytostatic-sensibility as seen with the two different methods. It seems to be possible, according to these investigations, to use enzyme-histochemical parameters from organ culture for inquiry of cytostatic-sensibility of tumors.", "contents": "[Investigations for using cell- and organ culture in sensibility tests for cytostatics in human ovarial-carcinomas (author's transl)]. Comparison was proved from meaning of cell culture and organ culture in therapeutic sensibility tests for cytostatics in ten human ovarial-carcinomas. The sensibility for cytostatics was determined with morphological criteria in cell culture and with the activity-patterns of some enzymes (lactate-dehydrogenase, NADH-diahorase) in organ culture. In these investigations the tumors have shown approximate conformity in the state to cytostatic-sensibility as seen with the two different methods. It seems to be possible, according to these investigations, to use enzyme-histochemical parameters from organ culture for inquiry of cytostatic-sensibility of tumors.", "PMID": 962500}
{"id": "pubmed23n0032_5099", "title": "[Testing of bronchial carcinoma for sensitivity against cytostatics in tissue culture (author's transl)].", "content": "Report on determinations of sensitivity against cytostatics of tumour cell cultures from 70 bronchial carcinomas and 5 neoplastic effusions. The take rate was 56%. Most tumors tested were in vitro sensible against vinblastin and cyclophosphamid and resistant (moderately or completely) against 5-fluoro-uracil and Methotrexat.", "contents": "[Testing of bronchial carcinoma for sensitivity against cytostatics in tissue culture (author's transl)]. Report on determinations of sensitivity against cytostatics of tumour cell cultures from 70 bronchial carcinomas and 5 neoplastic effusions. The take rate was 56%. Most tumors tested were in vitro sensible against vinblastin and cyclophosphamid and resistant (moderately or completely) against 5-fluoro-uracil and Methotrexat.", "PMID": 962501}
{"id": "pubmed23n0032_5100", "title": "[Contribution to stability test of daunoblastin solutions (author's transl)].", "content": "It is known that drugs are not unlimitedly stable. Dilutions of the antineoplastic agent daunoblastin used in the clinic and experimental work, were examined, whether they could be stored for several days. By means of impulscytophotometry, spectral-photometric measurements, counting of surviving cells and estimation of DNA-synthesis after culturing pieces of tissue in the presence of daunoblastin it could be established that this drug, diluted even four days ago, still possesses sufficient activity.", "contents": "[Contribution to stability test of daunoblastin solutions (author's transl)]. It is known that drugs are not unlimitedly stable. Dilutions of the antineoplastic agent daunoblastin used in the clinic and experimental work, were examined, whether they could be stored for several days. By means of impulscytophotometry, spectral-photometric measurements, counting of surviving cells and estimation of DNA-synthesis after culturing pieces of tissue in the presence of daunoblastin it could be established that this drug, diluted even four days ago, still possesses sufficient activity.", "PMID": 962502}
{"id": "pubmed23n0032_5101", "title": "[Studies for a mathematical model of the proliferation kinetics of mammary carcinomas by means of electronic data processing (author's transl)].", "content": "Continuing an earlier publication in which the growth of spontaneous mammary carcinomas in female mice of a C3H inbred strain was described, in the present paper the growth rate is expressed by a mathematical function, and is adapted by electronic data processing to the experimental values of 60 tumors classified into seven \"growth types\". For mathematical description the tumors were conveniently assumed to have a spherical shape, and the main reason  for the reduction in volume increase with increasing tumor size was thought to be insufficient supply with oxygen and the essential nutrients S. Including the experimental data of all carcinomas investigated, the minimum concentration of S necessary for mitotic activity of the tumor cells was calculated to be 65% of the normal level. As the average for all the tumors investigated, a time of at least 33 hrs is required for the tumor volume to double.", "contents": "[Studies for a mathematical model of the proliferation kinetics of mammary carcinomas by means of electronic data processing (author's transl)]. Continuing an earlier publication in which the growth of spontaneous mammary carcinomas in female mice of a C3H inbred strain was described, in the present paper the growth rate is expressed by a mathematical function, and is adapted by electronic data processing to the experimental values of 60 tumors classified into seven \"growth types\". For mathematical description the tumors were conveniently assumed to have a spherical shape, and the main reason  for the reduction in volume increase with increasing tumor size was thought to be insufficient supply with oxygen and the essential nutrients S. Including the experimental data of all carcinomas investigated, the minimum concentration of S necessary for mitotic activity of the tumor cells was calculated to be 65% of the normal level. As the average for all the tumors investigated, a time of at least 33 hrs is required for the tumor volume to double.", "PMID": 962503}
{"id": "pubmed23n0032_5102", "title": "Diagnostic value of chromosome examination in effusions from cancer patients.", "content": "Fifty nine effusions (6 from patients with benign diseases and 53 from cancer patients) were examined cytologically and cytogenetically. Only diploid cells were observed in the benign effusions. The majority of malignant effusions had chromosomal changes, but in three cases where cytologic examination had revealed the presence of malignant cells, only diploid metaphases were found. No false positive diagnoses were made either by the cytologic or by the cytogenetic technique. The concomitant use of cytologic and cytogenetic examinations has substantially improved the correctness of the diagnosis. If by cytologic examination 43 of the 53 effusions from cancer patients were diagnosed as positive, 4 as suspicious and 6 as negative, the combined results of the cytologic and cytogenetic examination can be summarized in 49 positive, 1 suspicious and 3 negative cases. The implications of these data for the medical practice are briefly discussed.", "contents": "Diagnostic value of chromosome examination in effusions from cancer patients. Fifty nine effusions (6 from patients with benign diseases and 53 from cancer patients) were examined cytologically and cytogenetically. Only diploid cells were observed in the benign effusions. The majority of malignant effusions had chromosomal changes, but in three cases where cytologic examination had revealed the presence of malignant cells, only diploid metaphases were found. No false positive diagnoses were made either by the cytologic or by the cytogenetic technique. The concomitant use of cytologic and cytogenetic examinations has substantially improved the correctness of the diagnosis. If by cytologic examination 43 of the 53 effusions from cancer patients were diagnosed as positive, 4 as suspicious and 6 as negative, the combined results of the cytologic and cytogenetic examination can be summarized in 49 positive, 1 suspicious and 3 negative cases. The implications of these data for the medical practice are briefly discussed.", "PMID": 962504}
{"id": "pubmed23n0032_5103", "title": "[In vitro testing and clinical experience with 5-fluorouracil in gynaecologic cancer (author's transl)].", "content": "201 pretherapeutic tests for sensitivity of gynaecologic cancers against 5-fluorouracil were performed. Only 8.5% of cases proved to be sensitive in tissue culture. Clinical results with 5-FU monotherapy were unsatisfactory. 5-FU should only be used very critically in gynaecologic neoplasims. The value of predictive tests for sensitivity against cytostatics in planning individualized selective chemotherapy is emphasized.", "contents": "[In vitro testing and clinical experience with 5-fluorouracil in gynaecologic cancer (author's transl)]. 201 pretherapeutic tests for sensitivity of gynaecologic cancers against 5-fluorouracil were performed. Only 8.5% of cases proved to be sensitive in tissue culture. Clinical results with 5-FU monotherapy were unsatisfactory. 5-FU should only be used very critically in gynaecologic neoplasims. The value of predictive tests for sensitivity against cytostatics in planning individualized selective chemotherapy is emphasized.", "PMID": 962505}
{"id": "pubmed23n0032_5104", "title": "[Substances with carcinogenic activity (second edition) (author's transl)].", "content": "A list of substances with carcinogenic activity is presented as a 2nd supplemented edition. The list includes synonyma and test routes of the substances and should be looked as information material for employers and managers in various fields of industry, agriculture etc., hygiene and occupational and health service. The list is available as a paper-back (265 pages).", "contents": "[Substances with carcinogenic activity (second edition) (author's transl)]. A list of substances with carcinogenic activity is presented as a 2nd supplemented edition. The list includes synonyma and test routes of the substances and should be looked as information material for employers and managers in various fields of industry, agriculture etc., hygiene and occupational and health service. The list is available as a paper-back (265 pages).", "PMID": 962506}
{"id": "pubmed23n0032_5105", "title": "[Sarcomas in syrian hamster, induced by an oncorna virus and cellfree transmissible (author's transl)].", "content": "1. Application of hamster papova virus to newborns of Syrian hamster has produced some s.c. sarcomas after a 5 to 6 month latency period, by virtue of the strong inducer effect of this papova virus to endogenous (latent) oncorna viruses. 2. Cellfree filtrates from a polymorphorus-cell sarcoma produced in this way, when applied to newborn hamsters of the spontaneously tumour-free hamster line HaP, again lead to sarcoma formation after a latency period of 3--8 months in about 20% of the animals; the same holds for cellfree filtrates of these cellfree induced sarcomas and their transplantation generations. 3. In these tumours C-type oncorna viruses, but no papova virus, could be demonstrated regularly. 4. The hamster specificity of this sarcoma virus is suggested by the complete absence of a tumorigenic effect of the cellfree filtrates from these hamster sarcomas in mice and rats. The preferential induction of hamster sarcomas by sarcoma filtrates, in conjunction with the fact that filtrates from hamster leukoses, indicates a certain difference between hamster leukemia and hamster sarcoma viruses.", "contents": "[Sarcomas in syrian hamster, induced by an oncorna virus and cellfree transmissible (author's transl)]. 1. Application of hamster papova virus to newborns of Syrian hamster has produced some s.c. sarcomas after a 5 to 6 month latency period, by virtue of the strong inducer effect of this papova virus to endogenous (latent) oncorna viruses. 2. Cellfree filtrates from a polymorphorus-cell sarcoma produced in this way, when applied to newborn hamsters of the spontaneously tumour-free hamster line HaP, again lead to sarcoma formation after a latency period of 3--8 months in about 20% of the animals; the same holds for cellfree filtrates of these cellfree induced sarcomas and their transplantation generations. 3. In these tumours C-type oncorna viruses, but no papova virus, could be demonstrated regularly. 4. The hamster specificity of this sarcoma virus is suggested by the complete absence of a tumorigenic effect of the cellfree filtrates from these hamster sarcomas in mice and rats. The preferential induction of hamster sarcomas by sarcoma filtrates, in conjunction with the fact that filtrates from hamster leukoses, indicates a certain difference between hamster leukemia and hamster sarcoma viruses.", "PMID": 962507}
{"id": "pubmed23n0032_5106", "title": "[Mesothelioma in syrian hamster. V. Macroscopic and histologic investigation of transplanted mesothelioma in solid and ascitic form (author's transl)].", "content": "Transplantable mesothelioma lines are described, which have been inoculated successively either s.c. or i.p. in solid and ascitic form. Increases of the degree of malignancy could be demonstrated for macroscopically biological parameters. The latent periods became shorter with increasing in vivo passages. In the histological picture, progressive dedifferentiation of the tumour cells with rising tendency towards infiltrative growth was observed.", "contents": "[Mesothelioma in syrian hamster. V. Macroscopic and histologic investigation of transplanted mesothelioma in solid and ascitic form (author's transl)]. Transplantable mesothelioma lines are described, which have been inoculated successively either s.c. or i.p. in solid and ascitic form. Increases of the degree of malignancy could be demonstrated for macroscopically biological parameters. The latent periods became shorter with increasing in vivo passages. In the histological picture, progressive dedifferentiation of the tumour cells with rising tendency towards infiltrative growth was observed.", "PMID": 962508}
{"id": "pubmed23n0032_5107", "title": "Ferritin as a surface marker for immunoscanning electron microscopy. observation of individual ferritin particles on erythrocytes.", "content": "This paper comprises a part of our study to examine whether ferritin can be used as an immunological surface marker for high resolution scanning electron microscopy. In the first part of the study individual ferritin particles in a sample of purified ferritin were demonstrated as spheres with a diameter of about 170 A by use of fixation by glutaraldehyde and thin coating by ion sputtering. In the second part of this study, ferritin particles as indicating the antigen sites of blood group A were also recognized on the erythrocytes by the same techniques as in the first part. Ferritin deposition differed in amount among the erythrocytes, but without regard to the shape of the cells. On the individual erythrocytes, heavier depositions tended to occur in the concavity of the cell. In conclusion, ferritin is useful as a surface marker in immunoscanning electron microscopy. It has the benefit, in comparison with the other markers hitherto reported, of allowing us to observe the fine features of the cell surface under immunological reaction.", "contents": "Ferritin as a surface marker for immunoscanning electron microscopy. observation of individual ferritin particles on erythrocytes. This paper comprises a part of our study to examine whether ferritin can be used as an immunological surface marker for high resolution scanning electron microscopy. In the first part of the study individual ferritin particles in a sample of purified ferritin were demonstrated as spheres with a diameter of about 170 A by use of fixation by glutaraldehyde and thin coating by ion sputtering. In the second part of this study, ferritin particles as indicating the antigen sites of blood group A were also recognized on the erythrocytes by the same techniques as in the first part. Ferritin deposition differed in amount among the erythrocytes, but without regard to the shape of the cells. On the individual erythrocytes, heavier depositions tended to occur in the concavity of the cell. In conclusion, ferritin is useful as a surface marker in immunoscanning electron microscopy. It has the benefit, in comparison with the other markers hitherto reported, of allowing us to observe the fine features of the cell surface under immunological reaction.", "PMID": 962509}
{"id": "pubmed23n0032_5108", "title": "Possible function of human brown adipose tissue as suggested by observation on perirenal brown fats from necropsy cases of variable age groups.", "content": "In 134 out of 180 perirenal fat samples (74%) derived from Japanese necropsy cases aged from 1 month to 86 years, the brown fat tissue persisted in variable amounts. Brown fat cells were classified into 6 types: Type 1 cells are fat-depleted cells filled with granular cytoplasm and are believed to be produced after oxidation of fat for heat production. Type 2 cells are small-locular cells suitable for rapid oxidation of fat droplets. Type 3 (middle-locular) and 4 (large-locular) represent fat-storage cells containing large amounts of fat. Type 5 cells are thought to be transitional forms between multilocular brown fat cells and monolocular white fat cells. Type 6 (cytoplasm-rich multilocular) cells, usually found together with Type 1 cells, are thought to be fat-depleting or -consuming cells, since in them fat droplets are reduced in number and size probably in consequence of oxidation of fat, but by contrast granular cytoplasm is increased in amount separating the individual fat droplets by thick cytoplasmic septa. The occurrence of Types 1 and/or 6 cells that has been revealed in 65 out of the total 180 samples (36%), suggests that the oxidation of fat for the thermogenesis proceeds in the brown fat tissue and that brown fat cells partially undergo fat depletion. In the present study, the thermogenesis of human brown fat tissue was suggested chiefly with regard to the occurrence of Types 1 and/or 6 cells. In the majority of perirenal fat samples from infants (1-11 months) relatively numerous Types 1 and 6 cells were frequently revealed together with Type 2 cells, suggesting rapid and active heat production in support of the view that in human infants the brown fat tissue may be thermogenetically active to maintain body temperature. In the same manner, marked ability to produce a considerable amount of heat was evidenced in  brown fat tissue of children and teenagers. In younger and elderly adults the frequency of occurrence and the amount of the perirenal brown fat tissue were decreased but Types 1 and/or 6 cells could be found in 17-40% of them, infrequently together, with Type 2 cells, suggesting persistence of the thermogenic activity with occasional large heat production especially in younger adults (20-39 years). Thus, the results obtained in this study have clarified that the human brown fat tissue can respond to stimuli given to the body by oxidation of stored fat even in the latest decades of life. In cases of death from burning, drowning, bleeding, drug poisoning etc., numerous Types 1 and/or 6 cells were found, suggesting that an active fat oxidation would take place in brown fat tissue assumedly as the result of the raised noradrenalin level in this tissue. The so-called small cytoplasmic cells found in perirenal fats from cases of death from liver cirrhosis and other causes were assumed to be atrophic fat-depleted brown fat cells.", "contents": "Possible function of human brown adipose tissue as suggested by observation on perirenal brown fats from necropsy cases of variable age groups. In 134 out of 180 perirenal fat samples (74%) derived from Japanese necropsy cases aged from 1 month to 86 years, the brown fat tissue persisted in variable amounts. Brown fat cells were classified into 6 types: Type 1 cells are fat-depleted cells filled with granular cytoplasm and are believed to be produced after oxidation of fat for heat production. Type 2 cells are small-locular cells suitable for rapid oxidation of fat droplets. Type 3 (middle-locular) and 4 (large-locular) represent fat-storage cells containing large amounts of fat. Type 5 cells are thought to be transitional forms between multilocular brown fat cells and monolocular white fat cells. Type 6 (cytoplasm-rich multilocular) cells, usually found together with Type 1 cells, are thought to be fat-depleting or -consuming cells, since in them fat droplets are reduced in number and size probably in consequence of oxidation of fat, but by contrast granular cytoplasm is increased in amount separating the individual fat droplets by thick cytoplasmic septa. The occurrence of Types 1 and/or 6 cells that has been revealed in 65 out of the total 180 samples (36%), suggests that the oxidation of fat for the thermogenesis proceeds in the brown fat tissue and that brown fat cells partially undergo fat depletion. In the present study, the thermogenesis of human brown fat tissue was suggested chiefly with regard to the occurrence of Types 1 and/or 6 cells. In the majority of perirenal fat samples from infants (1-11 months) relatively numerous Types 1 and 6 cells were frequently revealed together with Type 2 cells, suggesting rapid and active heat production in support of the view that in human infants the brown fat tissue may be thermogenetically active to maintain body temperature. In the same manner, marked ability to produce a considerable amount of heat was evidenced in  brown fat tissue of children and teenagers. In younger and elderly adults the frequency of occurrence and the amount of the perirenal brown fat tissue were decreased but Types 1 and/or 6 cells could be found in 17-40% of them, infrequently together, with Type 2 cells, suggesting persistence of the thermogenic activity with occasional large heat production especially in younger adults (20-39 years). Thus, the results obtained in this study have clarified that the human brown fat tissue can respond to stimuli given to the body by oxidation of stored fat even in the latest decades of life. In cases of death from burning, drowning, bleeding, drug poisoning etc., numerous Types 1 and/or 6 cells were found, suggesting that an active fat oxidation would take place in brown fat tissue assumedly as the result of the raised noradrenalin level in this tissue. The so-called small cytoplasmic cells found in perirenal fats from cases of death from liver cirrhosis and other causes were assumed to be atrophic fat-depleted brown fat cells.", "PMID": 962510}
{"id": "pubmed23n0032_5109", "title": "Ultrastructural and cytochemical studies on the remodelling of the tracheal cartilage.", "content": "Ultrastructural and cytochemical studies on the remodelling of the rat tracheal cartilage have been carried out. The thickness of the tracheal cartilage was constant, during the observation periods (1 to 54 days after birth). The external perichondrium of the tracheal cartilage consisted of active fibroblasts and intercellular fibrils. The inner part of this perichondrium was a chondrogenic layer, where appositional growth was taking place. On the other hand, the internal perichondrium contained fibroblast-like cells, which were nearly twice as large as the external perichondrial fibroblasts in size and were arranged in three or four layers. The cells had well developed organella and large vacuoles which contained numerous fragments of  fibrils and/or glycosaminoglycan. Many cytoplasmic processes protruded to the cartilage matrix, where the intercellular fibrils were particularly irregular in arrangement. Some vacuoles included collagen fibrils. Based on an intense acid phosphatase activity in these vacuoles and other findings, the fibrils were thought to be phagocytosed collagen of the cartilage matrix. An extensive alkaline phosphatase activity was demonstrated on the plasma membrane of fibroblasts and chondroblasts in the external perichondrium. The present investigation revealed distinct functional difference between the external and internal perichondrium of the tracheal cartilage. It is resorbed at the internal perichondrium, while it appositionally grows at the external perichondrium. The fibroblast-like cells of the internal perichondrium play an essential role in resorption of the matrix in cartilage remodelling.", "contents": "Ultrastructural and cytochemical studies on the remodelling of the tracheal cartilage. Ultrastructural and cytochemical studies on the remodelling of the rat tracheal cartilage have been carried out. The thickness of the tracheal cartilage was constant, during the observation periods (1 to 54 days after birth). The external perichondrium of the tracheal cartilage consisted of active fibroblasts and intercellular fibrils. The inner part of this perichondrium was a chondrogenic layer, where appositional growth was taking place. On the other hand, the internal perichondrium contained fibroblast-like cells, which were nearly twice as large as the external perichondrial fibroblasts in size and were arranged in three or four layers. The cells had well developed organella and large vacuoles which contained numerous fragments of  fibrils and/or glycosaminoglycan. Many cytoplasmic processes protruded to the cartilage matrix, where the intercellular fibrils were particularly irregular in arrangement. Some vacuoles included collagen fibrils. Based on an intense acid phosphatase activity in these vacuoles and other findings, the fibrils were thought to be phagocytosed collagen of the cartilage matrix. An extensive alkaline phosphatase activity was demonstrated on the plasma membrane of fibroblasts and chondroblasts in the external perichondrium. The present investigation revealed distinct functional difference between the external and internal perichondrium of the tracheal cartilage. It is resorbed at the internal perichondrium, while it appositionally grows at the external perichondrium. The fibroblast-like cells of the internal perichondrium play an essential role in resorption of the matrix in cartilage remodelling.", "PMID": 962511}
{"id": "pubmed23n0032_5110", "title": "A versatile method for purification of diphtheria toxoid for immunization purposes.", "content": "Diphtheriae growth supernatant.  The toxoid was quantitatively recovered from the immunosorbent in two fractions: the first in 0.005 M hydochloric acid and the second in 6' M urea containing 0.1 M HCL.  Both fractions were soluble in aqueous solvents, and both had a similar specific activity:  between 200 and 250 Lf/mg protein, and about 1.5 protective unit (ED50) per l Lf unit, as assayed in guinea pigs. Using the same procedure, the specific activity of a commerical toxoid preparation, purified and concentrated, was increrased two fold, from 130 Lf/mg to 260 Lf/mg protein.", "contents": "A versatile method for purification of diphtheria toxoid for immunization purposes. Diphtheriae growth supernatant.  The toxoid was quantitatively recovered from the immunosorbent in two fractions: the first in 0.005 M hydochloric acid and the second in 6' M urea containing 0.1 M HCL.  Both fractions were soluble in aqueous solvents, and both had a similar specific activity:  between 200 and 250 Lf/mg protein, and about 1.5 protective unit (ED50) per l Lf unit, as assayed in guinea pigs. Using the same procedure, the specific activity of a commerical toxoid preparation, purified and concentrated, was increrased two fold, from 130 Lf/mg to 260 Lf/mg protein.", "PMID": 962512}
{"id": "pubmed23n0032_5111", "title": "Application of the leukocyte migration inhibition (LMI) test in clinical management of diseases of the respiratory system.", "content": "Results of the leukocyte migration inhibition (LMI) test using RT23 tuberculin were compared with the skin reaction of tuberculin (OT), intensity of bacillary excretion and erythrocyte sedimentation rate. LMI tests were made at the beginning and toward the end of the courses of antituberculosis treatment. In patients suffering from sarcoidosis (BBS), the LMI test was performed using Kveim's antigen and RT23 tuberculin.", "contents": "Application of the leukocyte migration inhibition (LMI) test in clinical management of diseases of the respiratory system. Results of the leukocyte migration inhibition (LMI) test using RT23 tuberculin were compared with the skin reaction of tuberculin (OT), intensity of bacillary excretion and erythrocyte sedimentation rate. LMI tests were made at the beginning and toward the end of the courses of antituberculosis treatment. In patients suffering from sarcoidosis (BBS), the LMI test was performed using Kveim's antigen and RT23 tuberculin.", "PMID": 962513}
{"id": "pubmed23n0032_5112", "title": "Colony forming units (CFU) in bone marrow associated with heterotopic bone formation by epithelial cells grafted in mice.", "content": "The presence of bone marrow accompanying heterotopic bone induction in mice did not increase the number of endogenous spleen colonies. However, such bone marrow, as judged by the ability to form exogenous spleen colonies, contains colony-forming cells (CFC). No significant difference was found between the concentration of CFC in the induced and in the femoral bone marrow. This indicates a qualitative similarity between these two types of bone marrow inasfar as hemopoietic activity is concerned.", "contents": "Colony forming units (CFU) in bone marrow associated with heterotopic bone formation by epithelial cells grafted in mice. The presence of bone marrow accompanying heterotopic bone induction in mice did not increase the number of endogenous spleen colonies. However, such bone marrow, as judged by the ability to form exogenous spleen colonies, contains colony-forming cells (CFC). No significant difference was found between the concentration of CFC in the induced and in the femoral bone marrow. This indicates a qualitative similarity between these two types of bone marrow inasfar as hemopoietic activity is concerned.", "PMID": 962514}
{"id": "pubmed23n0032_5113", "title": "Immunoglobinuria in the acute phase of viral hepatitis in children and the influence of HBAg antigen.", "content": "Urinary excretion of immunoglobulins of the A, G and M classes was studied in 14 children suffering from viral hepatitis without HBAg antigenemia, and in 12 children with viral hepatitis accompanied by HBAg antigenemia. The control group consisted of 11 healthy children. Over fourfold increase in excretion of IgA, and only twofold increase in IgG were observed. The difference in excretion of A and G immunoglobulins, despite their similar molecular weight, was attributed to increased active excretion of IgA by the kidneys, probably due to stimulation by the virusemia. In no case were immunoglobulins of the M class encountered in the urine.", "contents": "Immunoglobinuria in the acute phase of viral hepatitis in children and the influence of HBAg antigen. Urinary excretion of immunoglobulins of the A, G and M classes was studied in 14 children suffering from viral hepatitis without HBAg antigenemia, and in 12 children with viral hepatitis accompanied by HBAg antigenemia. The control group consisted of 11 healthy children. Over fourfold increase in excretion of IgA, and only twofold increase in IgG were observed. The difference in excretion of A and G immunoglobulins, despite their similar molecular weight, was attributed to increased active excretion of IgA by the kidneys, probably due to stimulation by the virusemia. In no case were immunoglobulins of the M class encountered in the urine.", "PMID": 962515}
{"id": "pubmed23n0032_5114", "title": "GPT polymorphism in the Polish population.", "content": "In a sample of the Polish population numbering 703 subjects, three GPT types were encountered with the following frequencies: GPT1-1 0.265, GPT 2-1 0,509 and GPT 2-20.226. Frequencies of GPT1 and GPT2 genes were 0.519 and 0.481 respectively. Distribution of types in 217 mother-child pairs and in 12 families with 36 children was consistent with the hypothesis that the GPT system is dependent on a pair of codominant alleles and confirms that the GPT 1-1 and GPT 2-2 types are homozygotes, and GPT 2-1 is a heterozygote.", "contents": "GPT polymorphism in the Polish population. In a sample of the Polish population numbering 703 subjects, three GPT types were encountered with the following frequencies: GPT1-1 0.265, GPT 2-1 0,509 and GPT 2-20.226. Frequencies of GPT1 and GPT2 genes were 0.519 and 0.481 respectively. Distribution of types in 217 mother-child pairs and in 12 families with 36 children was consistent with the hypothesis that the GPT system is dependent on a pair of codominant alleles and confirms that the GPT 1-1 and GPT 2-2 types are homozygotes, and GPT 2-1 is a heterozygote.", "PMID": 962516}
{"id": "pubmed23n0032_5115", "title": "Frequency of occurrence of the Gm(21) factor in the Polish population.", "content": "In a sample of the Polish population of 610 subjects, frequency of the Gm(21) factor was determined and compared with the frequency of Gm(1). The Gm(21) factor was found with a frequency of 0.359, and Gm(1) with 0.370. Both factors were present in 35.7% of sera examined. Factor Gm(1) alone, unaccompanied by Gm(21), was encountered in 8 sera, and factor Gm(21) without Gm(1) only in one serum.", "contents": "Frequency of occurrence of the Gm(21) factor in the Polish population. In a sample of the Polish population of 610 subjects, frequency of the Gm(21) factor was determined and compared with the frequency of Gm(1). The Gm(21) factor was found with a frequency of 0.359, and Gm(1) with 0.370. Both factors were present in 35.7% of sera examined. Factor Gm(1) alone, unaccompanied by Gm(21), was encountered in 8 sera, and factor Gm(21) without Gm(1) only in one serum.", "PMID": 962517}
{"id": "pubmed23n0032_5116", "title": "Activity of cobalt-activated acylase and Australia antigen (HBAg) in children suffering from viral hepatitis.", "content": "Activity of Coiactivated acylase in the serum was investigated in 120 children aged 3 months to 15 years, divided into five age groups, suffering from viral hepatitis (VH). No differences were found in the mean values of acylase activity between the ages of 3 months and 6 years, and progressively rising values in older age groups. Statistical analysis showed significance of the difference between the youngest age group and the group of children aged 12-15 years. A study of the dynamics of acylase activity in the course of the disease in 20 children showed rapidly declining values of this enzyme activity. Presence of HBAg in the serum was demonstrated in 12 children, in whom levels of acylase activity in the serum were similar to those in children without HB antigenemia, but the small number of cases examined does not permit conclusions. Acylase activity increases with age. This enzyme activity was observed in the serum of 96%, and HBAg in 10% of the examined children.", "contents": "Activity of cobalt-activated acylase and Australia antigen (HBAg) in children suffering from viral hepatitis. Activity of Coiactivated acylase in the serum was investigated in 120 children aged 3 months to 15 years, divided into five age groups, suffering from viral hepatitis (VH). No differences were found in the mean values of acylase activity between the ages of 3 months and 6 years, and progressively rising values in older age groups. Statistical analysis showed significance of the difference between the youngest age group and the group of children aged 12-15 years. A study of the dynamics of acylase activity in the course of the disease in 20 children showed rapidly declining values of this enzyme activity. Presence of HBAg in the serum was demonstrated in 12 children, in whom levels of acylase activity in the serum were similar to those in children without HB antigenemia, but the small number of cases examined does not permit conclusions. Acylase activity increases with age. This enzyme activity was observed in the serum of 96%, and HBAg in 10% of the examined children.", "PMID": 962518}
{"id": "pubmed23n0032_5117", "title": "Electromyographic temporal analysis of gait: normal human locomotion.", "content": "The telemetered electromyographic (EMG) activity of pretibial muscles (tibialis anterior), triceps surae (lateral gastrocnemius), medial hamstring group and quadriceps (vastus lateralis) of 20 normal subjects was examined during locomotion. The ages of the subjects ranged from 8 to 72 years (mean, 37 years). A microswitch shoe was used to correlate the EMG activity with eight specific components of the gait cycle. Tibialis anterior showed two peaks of activity, the first at the swing-stance transition, the second at the stance-swing transition. Gastrocnemius showed a single peak of activity recorded during push-off. The medial hamstring showed its greatest activity during deceleration in the swing phase. Vastus lateralis demonstrated peak activity at the transition from swing to stance. The mean cadence was 106 steps per minute. Swing phase occupied 39.6% and stance phase 60.4% of the gait cycle.", "contents": "Electromyographic temporal analysis of gait: normal human locomotion. The telemetered electromyographic (EMG) activity of pretibial muscles (tibialis anterior), triceps surae (lateral gastrocnemius), medial hamstring group and quadriceps (vastus lateralis) of 20 normal subjects was examined during locomotion. The ages of the subjects ranged from 8 to 72 years (mean, 37 years). A microswitch shoe was used to correlate the EMG activity with eight specific components of the gait cycle. Tibialis anterior showed two peaks of activity, the first at the swing-stance transition, the second at the stance-swing transition. Gastrocnemius showed a single peak of activity recorded during push-off. The medial hamstring showed its greatest activity during deceleration in the swing phase. Vastus lateralis demonstrated peak activity at the transition from swing to stance. The mean cadence was 106 steps per minute. Swing phase occupied 39.6% and stance phase 60.4% of the gait cycle.", "PMID": 962568}
{"id": "pubmed23n0032_5118", "title": "Electromyographic temporal analysis of gait: hemiplegic locomotion.", "content": "The telemetered electromyographic (EMG)  activity of quadriceps, hamstrings, triceps surae and pretibial muscles on the affected side of 20 adult hemiplegic subjects was examined during locomotion. The subjects ranged in age from 29 to 68 years (mean, 52.1). Duration of the lesions ranged from 1 month to 8 years: in  11 subjects the duration of the lesions ranged from 1 to 9 months (mean, 4.9 months), and in the remaining  9 subjects from 1 to 8 years (mean, 4 years 2 months).  Shoes with five microswitches, two in the heel and three in the sole, were used to correlate the  EMG activity with eight specific components of the gait cycle. The results of the study showed a loss of the phasic pattern associated with normal locomotion. The hemiplegic subjects showed the greatest activity in the period of midstance. Expressed as a percentage of the total cycle, the mean stance time of the paretic lower limb was 67% and the mean swing time was 33%. The unaffected lower limb showed a stance phase of 80% and a swing phase of 20%.", "contents": "Electromyographic temporal analysis of gait: hemiplegic locomotion. The telemetered electromyographic (EMG)  activity of quadriceps, hamstrings, triceps surae and pretibial muscles on the affected side of 20 adult hemiplegic subjects was examined during locomotion. The subjects ranged in age from 29 to 68 years (mean, 52.1). Duration of the lesions ranged from 1 month to 8 years: in  11 subjects the duration of the lesions ranged from 1 to 9 months (mean, 4.9 months), and in the remaining  9 subjects from 1 to 8 years (mean, 4 years 2 months).  Shoes with five microswitches, two in the heel and three in the sole, were used to correlate the  EMG activity with eight specific components of the gait cycle. The results of the study showed a loss of the phasic pattern associated with normal locomotion. The hemiplegic subjects showed the greatest activity in the period of midstance. Expressed as a percentage of the total cycle, the mean stance time of the paretic lower limb was 67% and the mean swing time was 33%. The unaffected lower limb showed a stance phase of 80% and a swing phase of 20%.", "PMID": 962569}
{"id": "pubmed23n0032_5119", "title": "Physical training program for rehabilitating extremely obese patients.", "content": "Performance records of 22 extremely obese adult patients undergoing physical rehabilitation in a multi-disciplinary program were used to devise three progressive training protocols to which patients can be individually assigned. These protocols include \"fastest pace\" walking and jogging and are designed to rehabilitate patients to a two-mile continuous walk within three, five or seven weeks. Specific definition of progressions in distance/work session units are given and criteria for assigning patients to each protocol are discussed. The program allows  time for psychological as well as physiological adaptation and improves the rehabilitative process by eliminating much of the variability and regression previously attributed to patient manipulation.", "contents": "Physical training program for rehabilitating extremely obese patients. Performance records of 22 extremely obese adult patients undergoing physical rehabilitation in a multi-disciplinary program were used to devise three progressive training protocols to which patients can be individually assigned. These protocols include \"fastest pace\" walking and jogging and are designed to rehabilitate patients to a two-mile continuous walk within three, five or seven weeks. Specific definition of progressions in distance/work session units are given and criteria for assigning patients to each protocol are discussed. The program allows  time for psychological as well as physiological adaptation and improves the rehabilitative process by eliminating much of the variability and regression previously attributed to patient manipulation.", "PMID": 962570}
{"id": "pubmed23n0032_5120", "title": "Restraining forces in various designs of knee ankle orthoses: their placement and effect on the anatomical knee joint.", "content": "A biochemical evaluation was conducted on double upright knee ankle orthoses, which were instrumented with strain gauge transducers to determine the magnitudes of the restraining forces exerted on the leg. Measurements were made on six commonly used designs of orthoses worn by spinal cord injured persons ambulating with a swing-through gait. The measurements were used to determine distribution of forces on the limb  as well as their effect on anatomical knee shear. Based on the experimental data, the following basic principles of optimal orthosis design were identified: The forces required to stabilize the knee should be minimized by applying the stabilizing force as close as possible to the knee center, and by maintaining the anatomical knee as straight as possible. When the major portion of the knee stabilizing force is applied below the knee, the shear on the anatomical knee structures is markedly reduced. Further, the stabilizing forces should be well distributed over tolerant areas.", "contents": "Restraining forces in various designs of knee ankle orthoses: their placement and effect on the anatomical knee joint. A biochemical evaluation was conducted on double upright knee ankle orthoses, which were instrumented with strain gauge transducers to determine the magnitudes of the restraining forces exerted on the leg. Measurements were made on six commonly used designs of orthoses worn by spinal cord injured persons ambulating with a swing-through gait. The measurements were used to determine distribution of forces on the limb  as well as their effect on anatomical knee shear. Based on the experimental data, the following basic principles of optimal orthosis design were identified: The forces required to stabilize the knee should be minimized by applying the stabilizing force as close as possible to the knee center, and by maintaining the anatomical knee as straight as possible. When the major portion of the knee stabilizing force is applied below the knee, the shear on the anatomical knee structures is markedly reduced. Further, the stabilizing forces should be well distributed over tolerant areas.", "PMID": 962571}
{"id": "pubmed23n0032_5121", "title": "Sexual adjustment and feminine attractiveness among spinal cord injured women.", "content": "Responses to semistructured interviews with 31 spinal cord injured women yielded sexual adjustment scores and material concerning sexual compensation and orgasm, bowel and bladder programs, and methods of enhancing attractiveness. Sexual adjustment scores were unrelated to time since injury. Few subjects reported the need to  make special provision to control elimination during sexual activity. Openness and honesty with mates were stressed. Those with high sexual-adjustment scores compensated for lost sensation and mobility. Orgasm descriptions and most attractiveness-related behaviors resembled those of able-bodied women; some attractiveness behaviors specifically compensated for disability.", "contents": "Sexual adjustment and feminine attractiveness among spinal cord injured women. Responses to semistructured interviews with 31 spinal cord injured women yielded sexual adjustment scores and material concerning sexual compensation and orgasm, bowel and bladder programs, and methods of enhancing attractiveness. Sexual adjustment scores were unrelated to time since injury. Few subjects reported the need to  make special provision to control elimination during sexual activity. Openness and honesty with mates were stressed. Those with high sexual-adjustment scores compensated for lost sensation and mobility. Orgasm descriptions and most attractiveness-related behaviors resembled those of able-bodied women; some attractiveness behaviors specifically compensated for disability.", "PMID": 962572}
{"id": "pubmed23n0032_5122", "title": "Recent developments in opiate research and their implications for psychiatry.", "content": "Considerable progress in opiate research has been made during the last few years regarding the identification and localization of opiate receptors in vitro and in vivo, the analysis of drug-receptor interactions and the characterization of an endogenous ligand of the opiate receptor. There is little evidence that effects induced by chronic exposure to opiates - development of tolerance and dependence -are due to changes in opiate receptor mechanisms; it is supposed that the adaptive changes occur mainly in the chain of events triggered by the drug-receptor interaction. Such changes may be directly or indirectly related to the metabolism of neurotransmitters and/or cyclic nucleotides. The obvious links between physical and psychic equivalents of opiate dependence are discussed. Present data points to the significance of brain stem and limbic structures in both these processes, monoamines probably playing an important role. Relations between psychic manifestations of opiate addiction and mental disorders are pointed out.", "contents": "Recent developments in opiate research and their implications for psychiatry. Considerable progress in opiate research has been made during the last few years regarding the identification and localization of opiate receptors in vitro and in vivo, the analysis of drug-receptor interactions and the characterization of an endogenous ligand of the opiate receptor. There is little evidence that effects induced by chronic exposure to opiates - development of tolerance and dependence -are due to changes in opiate receptor mechanisms; it is supposed that the adaptive changes occur mainly in the chain of events triggered by the drug-receptor interaction. Such changes may be directly or indirectly related to the metabolism of neurotransmitters and/or cyclic nucleotides. The obvious links between physical and psychic equivalents of opiate dependence are discussed. Present data points to the significance of brain stem and limbic structures in both these processes, monoamines probably playing an important role. Relations between psychic manifestations of opiate addiction and mental disorders are pointed out.", "PMID": 962573}
{"id": "pubmed23n0032_5123", "title": "[Potassium activity in the cat cortex: experimental epilepsy].", "content": "Two mechanisms are discussed which link extracellular potassium accumulation and epileptogenic neuronal hyperactivity in the cortex. The potassium concentration (aK) of the environment of a repetitively discharging membrane can increase sufficiently for a supra-threshold depolarization at afferent erminals. This can explain the finding of ectopic spike generation and the antidromic breakthrough in thalamo-cortical projections after a primary cortical discharge. Spread and recurrent enhancement of excitatory drives may be the result of this mechanism. Initiation and termination of seizure is not explained by potassium accumulation. There is a ceiling level in potassium of about 10 mequ/1 which is strictly maintained during normal as well as epileptiform activity. This level is probably not high enough for depolarizing inactivation of neuronal membranes. Stimulation of cortical afferents can have a dual effect on aK. After a primary shortlasting increase, aK can reach subnormal values. This is possibly brought about by a stimulated re-uptake of K+. Seizures can be initiated at these subnormal levels. The effect of the re-uptake e.g. hyperpolarization of terminal afferents and increase of evoked transmitter release is discussed for the initiation for paroxysmal activity.", "contents": "[Potassium activity in the cat cortex: experimental epilepsy]. Two mechanisms are discussed which link extracellular potassium accumulation and epileptogenic neuronal hyperactivity in the cortex. The potassium concentration (aK) of the environment of a repetitively discharging membrane can increase sufficiently for a supra-threshold depolarization at afferent erminals. This can explain the finding of ectopic spike generation and the antidromic breakthrough in thalamo-cortical projections after a primary cortical discharge. Spread and recurrent enhancement of excitatory drives may be the result of this mechanism. Initiation and termination of seizure is not explained by potassium accumulation. There is a ceiling level in potassium of about 10 mequ/1 which is strictly maintained during normal as well as epileptiform activity. This level is probably not high enough for depolarizing inactivation of neuronal membranes. Stimulation of cortical afferents can have a dual effect on aK. After a primary shortlasting increase, aK can reach subnormal values. This is possibly brought about by a stimulated re-uptake of K+. Seizures can be initiated at these subnormal levels. The effect of the re-uptake e.g. hyperpolarization of terminal afferents and increase of evoked transmitter release is discussed for the initiation for paroxysmal activity.", "PMID": 962574}
{"id": "pubmed23n0032_5124", "title": "[Psychiatry, morphology and behaviour genetics (author's transl)].", "content": "No organic substrate is known for endogenous psychoses, neuroses and behaviour disorders. Therefore genetic studies have to depart from the behavioural level. This approach has yielded the empirical risk figures which depend on genetic and environmental factors. Beyond this level behaviour genetics endeavours to clarify the genetic mechanisms underlying normal and abnormal behaviour. In the schizophrenias f.i. several stretegies have been developed to approach the genetic basis: Mathematial models as to the mode of inheritance; use of modified classificatory and diagnostic criteria for genetic analysis; search for phenomena representing steps between genotype and phenotype and showing a clear mode of inheritance, f.i. biochemical or electrophysiological characters. In case the neurotransmitters are not changed in quality or total quantity but in compartmentation in the genetic defect might be localized in the pre- or postsynaptic membranes or receptors. Genetic factors are also involved in nonpsychotic behaviour disorders but to a lesser degree. They can be demonstrated even in psychoses with somatic background.", "contents": "[Psychiatry, morphology and behaviour genetics (author's transl)]. No organic substrate is known for endogenous psychoses, neuroses and behaviour disorders. Therefore genetic studies have to depart from the behavioural level. This approach has yielded the empirical risk figures which depend on genetic and environmental factors. Beyond this level behaviour genetics endeavours to clarify the genetic mechanisms underlying normal and abnormal behaviour. In the schizophrenias f.i. several stretegies have been developed to approach the genetic basis: Mathematial models as to the mode of inheritance; use of modified classificatory and diagnostic criteria for genetic analysis; search for phenomena representing steps between genotype and phenotype and showing a clear mode of inheritance, f.i. biochemical or electrophysiological characters. In case the neurotransmitters are not changed in quality or total quantity but in compartmentation in the genetic defect might be localized in the pre- or postsynaptic membranes or receptors. Genetic factors are also involved in nonpsychotic behaviour disorders but to a lesser degree. They can be demonstrated even in psychoses with somatic background.", "PMID": 962575}
{"id": "pubmed23n0032_5125", "title": "[Body-centered group psychotherapy in acute schizophrenics. Evaluation by rating of ego functions (Bellak et al., 1973)].", "content": "37 acute schizophrenic inpatients were studied in respect to the therapeutic effect of the body-centered group psychotherapy (k\u00f6rperzentrierte Gruppenpsychotherapie). The evaluation was made by the rating of ego functions (Bellak et al., 1973). The acute schizophrenic patients with somatic disturbances (functional ones, body illusions and halluzinations, depersonalisation) turned out to make significantly better progress in ego functions (p less than 0.05) than the control sample.", "contents": "[Body-centered group psychotherapy in acute schizophrenics. Evaluation by rating of ego functions (Bellak et al., 1973)]. 37 acute schizophrenic inpatients were studied in respect to the therapeutic effect of the body-centered group psychotherapy (k\u00f6rperzentrierte Gruppenpsychotherapie). The evaluation was made by the rating of ego functions (Bellak et al., 1973). The acute schizophrenic patients with somatic disturbances (functional ones, body illusions and halluzinations, depersonalisation) turned out to make significantly better progress in ego functions (p less than 0.05) than the control sample.", "PMID": 962576}
{"id": "pubmed23n0032_5126", "title": "[Sex chromosome aberration screening among male psychiatric patients (author's transl)].", "content": "The authors report of sex chromosome aberration screenings among the patients of the male psychiatric department, University Medical School, Pecs. 310 patients were investigated. The X-chromatin was detected in buccal smears with thionin-staining and the Y-chromatin in peripheric blood smears with quinacrin-staining by the help of fluorescentoptical technique. Two Klinefelter-patients and one YY-patient were diagnostized. The Klinefelter-patients were psychopaths and mentally subnormal, the YY-patient was a paranoid schizophrenic. The incidence of Klinefelter syndrome is 0.64%, that of the YY syndrome is 0.32%. Mental relations of sex chromosome aberrations are discussed in detail.", "contents": "[Sex chromosome aberration screening among male psychiatric patients (author's transl)]. The authors report of sex chromosome aberration screenings among the patients of the male psychiatric department, University Medical School, Pecs. 310 patients were investigated. The X-chromatin was detected in buccal smears with thionin-staining and the Y-chromatin in peripheric blood smears with quinacrin-staining by the help of fluorescentoptical technique. Two Klinefelter-patients and one YY-patient were diagnostized. The Klinefelter-patients were psychopaths and mentally subnormal, the YY-patient was a paranoid schizophrenic. The incidence of Klinefelter syndrome is 0.64%, that of the YY syndrome is 0.32%. Mental relations of sex chromosome aberrations are discussed in detail.", "PMID": 962577}
{"id": "pubmed23n0032_5127", "title": "[Excretion of acetyl urea in milk following oral 14C-acetyl urea administration to dairy cows].", "content": "2 experimental cows were fed acetyl urea several weeks before the trial was started. The first cow received a daily amount of 200 g and the second cow 855 g. On the first day of experiment both cows were given 5 mCi of 14C acetyl urea intraruminally. Up to 6 hrs after the beginning of the experiment acetyl urea in blood plasma was shown to contain a higher proportion of 14C activity than urea. 0.21 g urea and 0.18 g acetyl urea were contained in 1 kg of milk from cow No 1 while 1 kg of milk from cow No 2 contained 0.18 g urea and 0.12 g acetyl urea. The feeding of acetyl urea to dairy cows is not recommended on the basis of the fact that any further contamination of human nutrition with foreign substances should be possibly avoided.", "contents": "[Excretion of acetyl urea in milk following oral 14C-acetyl urea administration to dairy cows]. 2 experimental cows were fed acetyl urea several weeks before the trial was started. The first cow received a daily amount of 200 g and the second cow 855 g. On the first day of experiment both cows were given 5 mCi of 14C acetyl urea intraruminally. Up to 6 hrs after the beginning of the experiment acetyl urea in blood plasma was shown to contain a higher proportion of 14C activity than urea. 0.21 g urea and 0.18 g acetyl urea were contained in 1 kg of milk from cow No 1 while 1 kg of milk from cow No 2 contained 0.18 g urea and 0.12 g acetyl urea. The feeding of acetyl urea to dairy cows is not recommended on the basis of the fact that any further contamination of human nutrition with foreign substances should be possibly avoided.", "PMID": 962578}
{"id": "pubmed23n0032_5128", "title": "[Effect of zinc deficiency and varying zinc supplements on resorption and retention in dairy cows].", "content": "5 lactating cows were used in a trial to investigate the process of Zn absorption and Zn retention under conditions of Zn deficiency and variations in Zn supplementation. The apparent Zn absorption was found to be markedly increased if the animals were fed a Zn deficient semisynthetic diet containing 6 mg Zn per kg of dry matter during the depletion period. Apparent Zn absorption continued to rise with continuing Zn depletion. Over a period of 6 weeks of Zn depletion the mean rate of Zn absorption was 58% with increases from 51% to 64% between the 1st and 6th week of experiment. After 19 weeks of Zn depletion 75% of the supplemented zinc were absorbed. With a Zn supply of 22 ppm and 54 ppm the rate of apparent Zn absorption decreased to 51% and 44% while still higher Zn supplements, up to 436 ppm, produced only a slight further decline down to 40%. The absolute Zn balances were shown to be directly associated with the level of Zn supply, all the more so because a linear relationship was found to exist between the levels of alimentary zinc supplementation and absolute Zn retention. Under conditions of Zn deficiency the cows exhibited a negative Zn balance, where animals with the highest milk yields showed the most negative Zn balances and remained for the longest period in the negative phase. With increasing periods of Zn depletion a homeostatic mechanism of Zn regulation in the organism of the animals became more and more pronounced so that at advanced stages of Zn depletion Zn retention of some extent occurred.", "contents": "[Effect of zinc deficiency and varying zinc supplements on resorption and retention in dairy cows]. 5 lactating cows were used in a trial to investigate the process of Zn absorption and Zn retention under conditions of Zn deficiency and variations in Zn supplementation. The apparent Zn absorption was found to be markedly increased if the animals were fed a Zn deficient semisynthetic diet containing 6 mg Zn per kg of dry matter during the depletion period. Apparent Zn absorption continued to rise with continuing Zn depletion. Over a period of 6 weeks of Zn depletion the mean rate of Zn absorption was 58% with increases from 51% to 64% between the 1st and 6th week of experiment. After 19 weeks of Zn depletion 75% of the supplemented zinc were absorbed. With a Zn supply of 22 ppm and 54 ppm the rate of apparent Zn absorption decreased to 51% and 44% while still higher Zn supplements, up to 436 ppm, produced only a slight further decline down to 40%. The absolute Zn balances were shown to be directly associated with the level of Zn supply, all the more so because a linear relationship was found to exist between the levels of alimentary zinc supplementation and absolute Zn retention. Under conditions of Zn deficiency the cows exhibited a negative Zn balance, where animals with the highest milk yields showed the most negative Zn balances and remained for the longest period in the negative phase. With increasing periods of Zn depletion a homeostatic mechanism of Zn regulation in the organism of the animals became more and more pronounced so that at advanced stages of Zn depletion Zn retention of some extent occurred.", "PMID": 962579}
{"id": "pubmed23n0032_5129", "title": "[Relationship between food protein quality and adrenal cortex function. 6. Interrelationship between aminotransferase activity (GPT1, GOT2) and the status of adrenal cortex function].", "content": "Growing male Albino rats were adrenalectomized and then treated with corticosterone in an attempt to determine the activity of amino transferases (GPT and GOT) in the liver and in the mucosa of the small intestine over a period during which the animals received dietary proteins of varying quality. However, statistically significant differences in the enzyme activity were established for only those groups in which the animals exhibited differences in the functional state of the adrenal cortex. Differences due to changes in the quality of the dietary protein were not observed. From this was concluded that the activity of the amino transferases in the tissues is largely dependent on the action of corticosteroids.", "contents": "[Relationship between food protein quality and adrenal cortex function. 6. Interrelationship between aminotransferase activity (GPT1, GOT2) and the status of adrenal cortex function]. Growing male Albino rats were adrenalectomized and then treated with corticosterone in an attempt to determine the activity of amino transferases (GPT and GOT) in the liver and in the mucosa of the small intestine over a period during which the animals received dietary proteins of varying quality. However, statistically significant differences in the enzyme activity were established for only those groups in which the animals exhibited differences in the functional state of the adrenal cortex. Differences due to changes in the quality of the dietary protein were not observed. From this was concluded that the activity of the amino transferases in the tissues is largely dependent on the action of corticosteroids.", "PMID": 962580}
{"id": "pubmed23n0032_5130", "title": "[Relationship between food protein quality and adrenal cortex function. 7. Relationship between activity of selected liver and small intestine hydrolases and adrenal cortex function].", "content": "Male Wistar rats received 10% true digestible crude protein of varying quality (maize gluten (MK) = low protein quality; maize gluten + lysine, tryptophan, threonine, isoleucine (MK + AS) = high protein quality) for a period of three weeks. The trial was carried out to investigate the activity of the two enzymes leucine-arylamidase (LNA) and aminotripeptidase (TP) in the liver and the mucosa of the small intestine and that of alpha and gamma amylases and of total amylases in the mucosa of the small intestine in animals exhibiting differences in the functional state of their adrenal cortices (adrenalectomized animals (AE), normal rats (N) and animals receiving additional treatment with cortisol (KS). It was found that mucosal LNA and gamma amylase did not respond to functional changes in the adrenal cortex. Mucosal LNA had a higher activity if the dietary protein was of low quality and a lower activity if the protein quality was high. The level of liver LNA activity was significantly lower in N and KS animals than in AE animals. The highest activity of mucosal and liver TP was found in KS animals; it was lowest in N animals. Statistically significant differences brought about by changes in the quality of dietary proteins were only found to occur in the KS group. The activity of alpha amylase and total amylases was found to be lowest in N animals; it was significantly higher in animals of the AE and KS groups. Animals of the N and KS groups receiving MK + AS exhibited a significantly higher level in their alpha and total amylase activities than animals in the same group receiving MK. Here, the \"permissive action\" of glucocorticosteroids on metabolically active hydrolases is clearly evident. The pattern of mucosal LNA activity in the small intestine and that of gamma amylase may be regarded as further characteristic evidence for the digestive action of these enzymes. Liver LNA and liver TP as well as mucosal TP and alpha amylase acted like metabolic enzymes.", "contents": "[Relationship between food protein quality and adrenal cortex function. 7. Relationship between activity of selected liver and small intestine hydrolases and adrenal cortex function]. Male Wistar rats received 10% true digestible crude protein of varying quality (maize gluten (MK) = low protein quality; maize gluten + lysine, tryptophan, threonine, isoleucine (MK + AS) = high protein quality) for a period of three weeks. The trial was carried out to investigate the activity of the two enzymes leucine-arylamidase (LNA) and aminotripeptidase (TP) in the liver and the mucosa of the small intestine and that of alpha and gamma amylases and of total amylases in the mucosa of the small intestine in animals exhibiting differences in the functional state of their adrenal cortices (adrenalectomized animals (AE), normal rats (N) and animals receiving additional treatment with cortisol (KS). It was found that mucosal LNA and gamma amylase did not respond to functional changes in the adrenal cortex. Mucosal LNA had a higher activity if the dietary protein was of low quality and a lower activity if the protein quality was high. The level of liver LNA activity was significantly lower in N and KS animals than in AE animals. The highest activity of mucosal and liver TP was found in KS animals; it was lowest in N animals. Statistically significant differences brought about by changes in the quality of dietary proteins were only found to occur in the KS group. The activity of alpha amylase and total amylases was found to be lowest in N animals; it was significantly higher in animals of the AE and KS groups. Animals of the N and KS groups receiving MK + AS exhibited a significantly higher level in their alpha and total amylase activities than animals in the same group receiving MK. Here, the \"permissive action\" of glucocorticosteroids on metabolically active hydrolases is clearly evident. The pattern of mucosal LNA activity in the small intestine and that of gamma amylase may be regarded as further characteristic evidence for the digestive action of these enzymes. Liver LNA and liver TP as well as mucosal TP and alpha amylase acted like metabolic enzymes.", "PMID": 962581}
{"id": "pubmed23n0032_5131", "title": "[Effect of endogenous factors on ash concentration and contents of calcium, magnesium, potassium, sodium, phosphorus, zinc, iron, copper and manganese in the bristles of pigs. 5. Mineral composition of pig bristels influenced by pregnancy and lactation].", "content": "77 sows were used in a trial bristles were taken from these animals (a) on the 84th day of pregnancy, (b) on the day of gestation and (c) on the 42nd day of lactation. The average ash content of the bristles was 1.23%. It was not found to be influenced by gestation. Before gestation the Ca content of the bristles rose significantly while the Na and K content decreased (P less than 0.05). The Mg, P and Cu content of the bristles increased during lactation whereas the F content fell significantly. The concentrations of all other elements did not change significantly in the phase of pregnancy and during lactation.", "contents": "[Effect of endogenous factors on ash concentration and contents of calcium, magnesium, potassium, sodium, phosphorus, zinc, iron, copper and manganese in the bristles of pigs. 5. Mineral composition of pig bristels influenced by pregnancy and lactation]. 77 sows were used in a trial bristles were taken from these animals (a) on the 84th day of pregnancy, (b) on the day of gestation and (c) on the 42nd day of lactation. The average ash content of the bristles was 1.23%. It was not found to be influenced by gestation. Before gestation the Ca content of the bristles rose significantly while the Na and K content decreased (P less than 0.05). The Mg, P and Cu content of the bristles increased during lactation whereas the F content fell significantly. The concentrations of all other elements did not change significantly in the phase of pregnancy and during lactation.", "PMID": 962582}
{"id": "pubmed23n0032_5132", "title": "[Nutritional value of wine yeast for sheep and swine].", "content": "A digestibility trials was carried out with male sheep and one with fattening pigs to investigate the digestibility of wine yeast. 4 two-year-old male sheep and 5 castrated male pigs (live weight approximately 80 kgs) were used as experimental animals. The following digestibility data (%) were established on the sheep: organic matter: 78.9; energy: 79.1; crude protein: 61.1; crude fat: 88.8; crude fibre: 88.8; NFE 86.1. The corresponding data for the pigs were 55.4; 46.3; 22.7; 12.1; 92.7; 69.8. Thus, the resulting energy concentration was approximately 630 EFr units or 405 EFs units and the content of digestible crude protein in the dry matter was about 16% for ruminants and 6% in the pigs.", "contents": "[Nutritional value of wine yeast for sheep and swine]. A digestibility trials was carried out with male sheep and one with fattening pigs to investigate the digestibility of wine yeast. 4 two-year-old male sheep and 5 castrated male pigs (live weight approximately 80 kgs) were used as experimental animals. The following digestibility data (%) were established on the sheep: organic matter: 78.9; energy: 79.1; crude protein: 61.1; crude fat: 88.8; crude fibre: 88.8; NFE 86.1. The corresponding data for the pigs were 55.4; 46.3; 22.7; 12.1; 92.7; 69.8. Thus, the resulting energy concentration was approximately 630 EFr units or 405 EFs units and the content of digestible crude protein in the dry matter was about 16% for ruminants and 6% in the pigs.", "PMID": 962583}
{"id": "pubmed23n0032_5133", "title": "[Secretion of endogenous amino acids in the gastrointestinal tract and amino acid resorption in the swine].", "content": "A trial was performed with 2 fistula pigs (each with 2 fistulas, one located about 30 cm below the pyloric orifice and the other at the end of the small intestine). Animal A received a casein diet containing 14% crude protein for a period of 2 weeks before the tracer amino acid was administered. Animal B received the same diet for a period of 10 days and was then fed a diet (at the same protein level) containing gluten as sole protein source. The two tracer amino acids, 14C-U-L-leucine and 3H-4,5-(N)-L-lysine, were injected intravenously. The passage rates for dry matter, organic matter and N measured at the beginning of the small intestine were higher than the rate of intake. The rate of passage of amino acids was also found to be increased relative to the rate of intake. In general, this increase involved the non-essential amino acids to a much larger extent. A considerable proportion of the amino acids passing into the large intestine is not excreted with the faeces but is probably converted in catabolic processes. It is for this reason that any values for the efficiency of amino acid absorption calculated on the basis of data on the faecal excretion of amino acids will not provide conclusive evidence for the availability of dietary amino acids in processes of the intermediate metabolism. The rate of secretion of 3H and 14C radioactivity into the digesta of the small intestine was found to increase rapidly within 1-2 hrs after administration of the tracer amino acids. The 14C radioactivity detected was found to be almost exclusively derived from 14C leucine while only about 60% of the 3H activity found in the digesta of fistula I were shown to be bound to lysine. Labelled lysine and leucine (of endogenic origin) are absorbed into the small intestine at a slower rate (i.e. endogenic proteins are less efficiently digested) than the non-radioactive amino acids (of exogenic origin) so that a process of concentration of endogenic amino acids is observed towards the end of the small intestine.", "contents": "[Secretion of endogenous amino acids in the gastrointestinal tract and amino acid resorption in the swine]. A trial was performed with 2 fistula pigs (each with 2 fistulas, one located about 30 cm below the pyloric orifice and the other at the end of the small intestine). Animal A received a casein diet containing 14% crude protein for a period of 2 weeks before the tracer amino acid was administered. Animal B received the same diet for a period of 10 days and was then fed a diet (at the same protein level) containing gluten as sole protein source. The two tracer amino acids, 14C-U-L-leucine and 3H-4,5-(N)-L-lysine, were injected intravenously. The passage rates for dry matter, organic matter and N measured at the beginning of the small intestine were higher than the rate of intake. The rate of passage of amino acids was also found to be increased relative to the rate of intake. In general, this increase involved the non-essential amino acids to a much larger extent. A considerable proportion of the amino acids passing into the large intestine is not excreted with the faeces but is probably converted in catabolic processes. It is for this reason that any values for the efficiency of amino acid absorption calculated on the basis of data on the faecal excretion of amino acids will not provide conclusive evidence for the availability of dietary amino acids in processes of the intermediate metabolism. The rate of secretion of 3H and 14C radioactivity into the digesta of the small intestine was found to increase rapidly within 1-2 hrs after administration of the tracer amino acids. The 14C radioactivity detected was found to be almost exclusively derived from 14C leucine while only about 60% of the 3H activity found in the digesta of fistula I were shown to be bound to lysine. Labelled lysine and leucine (of endogenic origin) are absorbed into the small intestine at a slower rate (i.e. endogenic proteins are less efficiently digested) than the non-radioactive amino acids (of exogenic origin) so that a process of concentration of endogenic amino acids is observed towards the end of the small intestine.", "PMID": 962584}
{"id": "pubmed23n0032_5134", "title": "[Problem of nitrogen metabolism in monogastric animals].", "content": "A new method has been developed for determining the proportion of endogenic and exogenic faecal N by measuring the degree of specific labelling in the feeding-stuff (administered orally) and in the excreta (faeces and urine). The method has also been developed to find out whether the endogenic excretion of intestinal N is actually independent of the N intake. The present technique had been worked out on the basis of the model that had been originally established by Czarnetzki et al. in 1969 with the aim of characterizing processes of nitrogen metabolism in monogastric animals. As a result, a multi-compartment model was developed and a number of conclusions were derived from it. These conclusions were tested in 3 series of experiments on rats fed 15N labelled spring barley and 15N labelled feed yeast. Additionally, 15N labelled experimental rats were used receiving unlabelled extracted soya bean meal. The proportion of endogenic intestinal nitrogen (DVN) varied between 15-22% relative to the proportion of N ingested. The relative proportion of endogenic faecal N in the tota faecal N was found to be 100% in the case of the nitrogen-free diet. With increasing levels of dietary N this proportion lended to decrease down to a final value that was quite characteristic for each partitular feeding-stuff. The absolute amount of endogenic faecal N, however, was found to increase with increasing N levels. This shows that contrary to current views, DVN is not constant and is not independent of protein intake. Rather, a close relationship was found to exist between the level of N intake and DVN excretion (r = 0.951...0.972). So, the proportion of exogenic N contained in the faeces is much smaller than was previously assumed on the basis of the classical and regressive methods. These were based on the assumption that DVN is constant. Consequently, higher digestibility values will be obtained in comparison with those calculated on the basis of the previous methods. Digestibility data for spring barley increased from 83.9 to 95.6, i.e. by 11 digestibility units; for feed yeast there was an increase by 6.2 units, from 83.3 to 90.0; and for extracted soya bean meal an increase of 6.2 units was observed (from 90.7 to 96.9).", "contents": "[Problem of nitrogen metabolism in monogastric animals]. A new method has been developed for determining the proportion of endogenic and exogenic faecal N by measuring the degree of specific labelling in the feeding-stuff (administered orally) and in the excreta (faeces and urine). The method has also been developed to find out whether the endogenic excretion of intestinal N is actually independent of the N intake. The present technique had been worked out on the basis of the model that had been originally established by Czarnetzki et al. in 1969 with the aim of characterizing processes of nitrogen metabolism in monogastric animals. As a result, a multi-compartment model was developed and a number of conclusions were derived from it. These conclusions were tested in 3 series of experiments on rats fed 15N labelled spring barley and 15N labelled feed yeast. Additionally, 15N labelled experimental rats were used receiving unlabelled extracted soya bean meal. The proportion of endogenic intestinal nitrogen (DVN) varied between 15-22% relative to the proportion of N ingested. The relative proportion of endogenic faecal N in the tota faecal N was found to be 100% in the case of the nitrogen-free diet. With increasing levels of dietary N this proportion lended to decrease down to a final value that was quite characteristic for each partitular feeding-stuff. The absolute amount of endogenic faecal N, however, was found to increase with increasing N levels. This shows that contrary to current views, DVN is not constant and is not independent of protein intake. Rather, a close relationship was found to exist between the level of N intake and DVN excretion (r = 0.951...0.972). So, the proportion of exogenic N contained in the faeces is much smaller than was previously assumed on the basis of the classical and regressive methods. These were based on the assumption that DVN is constant. Consequently, higher digestibility values will be obtained in comparison with those calculated on the basis of the previous methods. Digestibility data for spring barley increased from 83.9 to 95.6, i.e. by 11 digestibility units; for feed yeast there was an increase by 6.2 units, from 83.3 to 90.0; and for extracted soya bean meal an increase of 6.2 units was observed (from 90.7 to 96.9).", "PMID": 962585}
{"id": "pubmed23n0032_5135", "title": "Effects of splenectomy on production of poly I:C induced serum interferon in rabbits.", "content": "Splenectomy in rabbits causes a temporary reduction in interferon responding capacity of Poly I:C, followed by a period of hyperreactivity. At the same time a fraction of interferon with slightly different physiocochemical properties emerges. This could indicate that the spleen plays a relatively important role in the interferon response to Poly I:C. Splenectomy could be followed by the development of a compensatory cell population responsible for the production of interferon with slightly different molecular weight, isoelectric point and stability in urea.", "contents": "Effects of splenectomy on production of poly I:C induced serum interferon in rabbits. Splenectomy in rabbits causes a temporary reduction in interferon responding capacity of Poly I:C, followed by a period of hyperreactivity. At the same time a fraction of interferon with slightly different physiocochemical properties emerges. This could indicate that the spleen plays a relatively important role in the interferon response to Poly I:C. Splenectomy could be followed by the development of a compensatory cell population responsible for the production of interferon with slightly different molecular weight, isoelectric point and stability in urea.", "PMID": 962586}
{"id": "pubmed23n0032_5136", "title": "Interferon induction and sensitivity as correlates to virulence of Venezuelan encephalitis viruses for hamsters.", "content": "Venezuelan encephalitis (VEE) virus strains, which differ in virulence for adult hamsters, were compared with respect to (a) sensitivity to hamster interferon (IF) in vitro and in vivo and (b) induction of IF in plasma and target tissues (spleen, bone marrow and brain) following subcutaneous inoculation. In vitro, in cultures of a continuous line of hamster kidney cells, hamster interferon inhibited the replication of a benign VEE strain (BeAr 35,645) more than another benign strain (TC-83 vaccine) or two hamster-virulent strains (68 U 201 and Trinidad donkey). In vivo, in hamsters given poly I: poly C 24 hours before virus to induce interferon formation, BeAr 35,645 and TC-83 virus infections were prevented more frequently than infections with virulent strains Trinidad donkey and 68U201. Benign VEE strains BeAr 35,645 and TC-83 induced only slightly lower concentrations of IF in plasma, bone marrow, spleen and brain than virulent strains Trinidad donkey, 63Z21 and 68U201. However, concentrations of infectious BeAr 35,645 virus were significantly lower in these tissues than virulent strains, resulting in higher ratios of IF: invectious virus, suggesting efficient interferon induction. Benign strain TC-83 showed irregular relationships between IF and infectious virus in plasma or blood and tissues, Splenectomy significantly depressed plasma plasma IF responses to TC-83 virus 20 to 30 hours after inoculation. Interferon appears to be a factor that influences virulence of VEE viruses for hamsters.", "contents": "Interferon induction and sensitivity as correlates to virulence of Venezuelan encephalitis viruses for hamsters. Venezuelan encephalitis (VEE) virus strains, which differ in virulence for adult hamsters, were compared with respect to (a) sensitivity to hamster interferon (IF) in vitro and in vivo and (b) induction of IF in plasma and target tissues (spleen, bone marrow and brain) following subcutaneous inoculation. In vitro, in cultures of a continuous line of hamster kidney cells, hamster interferon inhibited the replication of a benign VEE strain (BeAr 35,645) more than another benign strain (TC-83 vaccine) or two hamster-virulent strains (68 U 201 and Trinidad donkey). In vivo, in hamsters given poly I: poly C 24 hours before virus to induce interferon formation, BeAr 35,645 and TC-83 virus infections were prevented more frequently than infections with virulent strains Trinidad donkey and 68U201. Benign VEE strains BeAr 35,645 and TC-83 induced only slightly lower concentrations of IF in plasma, bone marrow, spleen and brain than virulent strains Trinidad donkey, 63Z21 and 68U201. However, concentrations of infectious BeAr 35,645 virus were significantly lower in these tissues than virulent strains, resulting in higher ratios of IF: invectious virus, suggesting efficient interferon induction. Benign strain TC-83 showed irregular relationships between IF and infectious virus in plasma or blood and tissues, Splenectomy significantly depressed plasma plasma IF responses to TC-83 virus 20 to 30 hours after inoculation. Interferon appears to be a factor that influences virulence of VEE viruses for hamsters.", "PMID": 962587}
{"id": "pubmed23n0032_5137", "title": "Epizootic congenital arthrogryposis-hydranencephaly syndrome in cattle: isolation of Akabane virus from affected fetuses.", "content": "Previous serolgoical studies strongly suggested Akabane virus to be the etiologic agent of epizootic abortion and congenital arthrogryposis-hydranencephaly in cattle, and this view was further corroborated in this study by the isolation of the virus from an aborted fetus in an epizootic of the disease and from a fetus extracted froma cow which was suggested by serologic tests to have a recent infection with the virus. The latter fetus had histological changes of encephalomyelitis and polymyositis, and specific antigens of Akabane virus was shown by the immunofluorescent technique in brain tissues as well as skeletal muscular tissues. The virus was recovered from various fetal tissues and fluids, and in relatively large amounts from brain, spinal cord, cerebral fluid, skeletal muscles and fetal placenta. The intracranial inoculation of suckling mice, 1-2 days of age, was the most sensitive system for Akabane virus isolation and HmLu-1, a continuous cell line from hamster lung, seemed almost as sensitive as suckling mice.", "contents": "Epizootic congenital arthrogryposis-hydranencephaly syndrome in cattle: isolation of Akabane virus from affected fetuses. Previous serolgoical studies strongly suggested Akabane virus to be the etiologic agent of epizootic abortion and congenital arthrogryposis-hydranencephaly in cattle, and this view was further corroborated in this study by the isolation of the virus from an aborted fetus in an epizootic of the disease and from a fetus extracted froma cow which was suggested by serologic tests to have a recent infection with the virus. The latter fetus had histological changes of encephalomyelitis and polymyositis, and specific antigens of Akabane virus was shown by the immunofluorescent technique in brain tissues as well as skeletal muscular tissues. The virus was recovered from various fetal tissues and fluids, and in relatively large amounts from brain, spinal cord, cerebral fluid, skeletal muscles and fetal placenta. The intracranial inoculation of suckling mice, 1-2 days of age, was the most sensitive system for Akabane virus isolation and HmLu-1, a continuous cell line from hamster lung, seemed almost as sensitive as suckling mice.", "PMID": 962588}
{"id": "pubmed23n0032_5138", "title": "Growth inhibition of human lymphoblastoid Daudi cells in vitro by interferon preparations.", "content": "Human interferon (HIF) preparations inhibited the propagation of Daudi cells in stationary suspension cultures, while a control preparation showed no such effect. The growth inhibition (= anticellular) activity of differently pretreated HIF preparations was determined by the reduction of 14C-labelled thymidine (14C-TDR) uptake in a microassay system. These studies demonstrated that the degree of anticellular activity is directly proportional to the antiviral activity of different HIF preparations. These preparations were obtained from peripheral leukocytes (lHIF) or diploid fibroblasts (fHIF). Together with gel chromatography results, and the sensitivity of the anticellular activity to tryptic digestion and heat inactivation, these results suggest that the anticellularly active substance is a protein which is very similar to, or identical with, interferon.", "contents": "Growth inhibition of human lymphoblastoid Daudi cells in vitro by interferon preparations. Human interferon (HIF) preparations inhibited the propagation of Daudi cells in stationary suspension cultures, while a control preparation showed no such effect. The growth inhibition (= anticellular) activity of differently pretreated HIF preparations was determined by the reduction of 14C-labelled thymidine (14C-TDR) uptake in a microassay system. These studies demonstrated that the degree of anticellular activity is directly proportional to the antiviral activity of different HIF preparations. These preparations were obtained from peripheral leukocytes (lHIF) or diploid fibroblasts (fHIF). Together with gel chromatography results, and the sensitivity of the anticellular activity to tryptic digestion and heat inactivation, these results suggest that the anticellularly active substance is a protein which is very similar to, or identical with, interferon.", "PMID": 962589}
{"id": "pubmed23n0032_5139", "title": "[Use of roentgenography for roentgeno-anatomic research].", "content": "The three-year-long experience of the use of electroroentgenography for anatomical investigations has shown that the details of the picture of anatomical objects which are insufficiently contrast in conventional roentgenograms may be revealed in electroroentgenograms. The obtained data speak of good prospects of application of electroentgenography in rentgenoanatomical studies.", "contents": "[Use of roentgenography for roentgeno-anatomic research]. The three-year-long experience of the use of electroroentgenography for anatomical investigations has shown that the details of the picture of anatomical objects which are insufficiently contrast in conventional roentgenograms may be revealed in electroroentgenograms. The obtained data speak of good prospects of application of electroentgenography in rentgenoanatomical studies.", "PMID": 962590}
{"id": "pubmed23n0032_5140", "title": "[X-ray anatomy of the maxillary sinuses].", "content": "In a complex roentgenological investigation of 12 anatomical preparations of the cranium and of 100 isolated maxillary bones (including multiplane roentgenography and the artificial contrasting of the antrum of Highmore) it has been established that in adult people the maxillary sinuses have as a rule, a shape of an irregular truncated pyramid (96%), rarely - a rounded shape. The standard roentgenograms of the cranium can reveal comparatively small portions of osseous walls of the maxillary sinuses. Special spot-films are necessary for studying the sinus walls on their full length.", "contents": "[X-ray anatomy of the maxillary sinuses]. In a complex roentgenological investigation of 12 anatomical preparations of the cranium and of 100 isolated maxillary bones (including multiplane roentgenography and the artificial contrasting of the antrum of Highmore) it has been established that in adult people the maxillary sinuses have as a rule, a shape of an irregular truncated pyramid (96%), rarely - a rounded shape. The standard roentgenograms of the cranium can reveal comparatively small portions of osseous walls of the maxillary sinuses. Special spot-films are necessary for studying the sinus walls on their full length.", "PMID": 962592}
{"id": "pubmed23n0032_5141", "title": "[X-ray anatomy of the inferior mesenteric artery according to findings of intravital angiography].", "content": "The article describes the results of roentgenological analysis of 905 patients having no pathological manifestations in the basin of the inferior mesenteric artery. The percutaneous catheterization of the aorta after Seldinger was made in all the patients. The authors have established that the typical variant of the artery is characterized by its appearance at the level of the third lumbar vertebrum body, by the branching of the trunk downwards to the left at an angle of 26,6 degrees +/- 0,2 (6-6,31 degrees) and by the main type of branching. The described rentgenological features of the vessel are helpful for the correct recognizing of the vessel in angiograms made in clinical conditions. The authors are likely to be the first to distinguish 10 variations of the branching of the inferior mesenteric artery and to give a detailed information on projection relationships of this vessel.", "contents": "[X-ray anatomy of the inferior mesenteric artery according to findings of intravital angiography]. The article describes the results of roentgenological analysis of 905 patients having no pathological manifestations in the basin of the inferior mesenteric artery. The percutaneous catheterization of the aorta after Seldinger was made in all the patients. The authors have established that the typical variant of the artery is characterized by its appearance at the level of the third lumbar vertebrum body, by the branching of the trunk downwards to the left at an angle of 26,6 degrees +/- 0,2 (6-6,31 degrees) and by the main type of branching. The described rentgenological features of the vessel are helpful for the correct recognizing of the vessel in angiograms made in clinical conditions. The authors are likely to be the first to distinguish 10 variations of the branching of the inferior mesenteric artery and to give a detailed information on projection relationships of this vessel.", "PMID": 962593}
{"id": "pubmed23n0032_5142", "title": "[Autoradiographic study of the early stages of human female meiosis in organ culture].", "content": "Incorporation of H3-thymidin in developing germinative cells and the movement of the label in successive stages of preprophase and prophase of meiosis were studied in the organic culture of the ovaries of 7-12 and 23 week human embryos by the method of autoradiography. H3-thymidin was incorporated by oognia and oocytes of the last stages of preleptotene with a thin uneven network of chromatin in the nucleus and by oocytes of the stage of leptotene and, rarely, zygotene. In oocytes with prochromosomes the label appeared within 12 hours. The cells labeled in the premeiotic DNA synthesis were changed into the zygotene stage within 4 days, into the pachytene stage- within 14 days. The development took 7-14 days from the last premitosis S-period to zygotene. Oogoniums divided two times for 7 days. Gz-period of oognia -1/2 of mitosis lasts an average of 10 hours. The S-period of oogonia lasts for about 9 hours.", "contents": "[Autoradiographic study of the early stages of human female meiosis in organ culture]. Incorporation of H3-thymidin in developing germinative cells and the movement of the label in successive stages of preprophase and prophase of meiosis were studied in the organic culture of the ovaries of 7-12 and 23 week human embryos by the method of autoradiography. H3-thymidin was incorporated by oognia and oocytes of the last stages of preleptotene with a thin uneven network of chromatin in the nucleus and by oocytes of the stage of leptotene and, rarely, zygotene. In oocytes with prochromosomes the label appeared within 12 hours. The cells labeled in the premeiotic DNA synthesis were changed into the zygotene stage within 4 days, into the pachytene stage- within 14 days. The development took 7-14 days from the last premitosis S-period to zygotene. Oogoniums divided two times for 7 days. Gz-period of oognia -1/2 of mitosis lasts an average of 10 hours. The S-period of oogonia lasts for about 9 hours.", "PMID": 962594}
{"id": "pubmed23n0032_5143", "title": "[Age and changes in the thyroid gland of A/He mice (morphometric and histochemical studies)].", "content": "Changes in the thyroid gland of mice A/He in postnatal ontogenesis were studied at the age of from 1 to 547 days by methods of morphometry and quantitative histochemistry. During the first days of life an active growth of the organ, accumulation of colloid, increase of the relative volume of the follicular epithelium in which the intensity of the histoenzymological reactions increased were observed. In the period of sex maturation the excretion of colloid was noticed as well as an increase of the height of follicular cells with activation of some of their enzymatic systems. Later on, while aging, the follicular cells gradually become flatter, the level of some enzymatic reactions of thyrocytes drops, the relative volume of colloid increases and its histochemical properties change. In the gland of animals older than 6-7 months well pronounced cystic changes were found.", "contents": "[Age and changes in the thyroid gland of A/He mice (morphometric and histochemical studies)]. Changes in the thyroid gland of mice A/He in postnatal ontogenesis were studied at the age of from 1 to 547 days by methods of morphometry and quantitative histochemistry. During the first days of life an active growth of the organ, accumulation of colloid, increase of the relative volume of the follicular epithelium in which the intensity of the histoenzymological reactions increased were observed. In the period of sex maturation the excretion of colloid was noticed as well as an increase of the height of follicular cells with activation of some of their enzymatic systems. Later on, while aging, the follicular cells gradually become flatter, the level of some enzymatic reactions of thyrocytes drops, the relative volume of colloid increases and its histochemical properties change. In the gland of animals older than 6-7 months well pronounced cystic changes were found.", "PMID": 962595}
{"id": "pubmed23n0032_5144", "title": "[Structure of the walls of the main arteries exposed to experimental hypergravitation and hypokinesia].", "content": "The structure of the wall of the carotid humeral and femoral arteries and the aorta was studied by histological methods in 260 rabbits under effects of positive longitudinal unendurable stresses, stresses of the training character and hypokinesia. Under study also was the structure of components of the aorta adventitia microcirculatory bed with the impregnation of tissue sections after V.V. Kuprijanov. The reversibility of morphological changes in the wall was studied after effects of the above factors and their different combinations. The experiments have shown the morphological changes in the wall to be of similar type and to consist of hyperelastosis and atrophy of the media under chronic stimuli. In the microcirculatory bed there occurred dilatation of postcapillaries and venules, diapedesic hemorrhages suggesting phenomena of stagnation. All the morphological changes were reversible speaking of great adaptive potentialities of the vascular system.", "contents": "[Structure of the walls of the main arteries exposed to experimental hypergravitation and hypokinesia]. The structure of the wall of the carotid humeral and femoral arteries and the aorta was studied by histological methods in 260 rabbits under effects of positive longitudinal unendurable stresses, stresses of the training character and hypokinesia. Under study also was the structure of components of the aorta adventitia microcirculatory bed with the impregnation of tissue sections after V.V. Kuprijanov. The reversibility of morphological changes in the wall was studied after effects of the above factors and their different combinations. The experiments have shown the morphological changes in the wall to be of similar type and to consist of hyperelastosis and atrophy of the media under chronic stimuli. In the microcirculatory bed there occurred dilatation of postcapillaries and venules, diapedesic hemorrhages suggesting phenomena of stagnation. All the morphological changes were reversible speaking of great adaptive potentialities of the vascular system.", "PMID": 962596}
{"id": "pubmed23n0032_5145", "title": "[Character of changes in the microcirculatory bed of the mesentery of the small intestine in experimental acute myocardial infarct].", "content": "On the first day of the development of acute coronary insufficiency there appears a spastic state of all the vascular system of the small intestine mesentery and a deceleration of the blood flow. On the third day after ligation of the coronary artery branch against the background of spasmed arterioles the alternation of sites of constricted and dilated capillaries, postcapillaries and venules was observed, i.e., there was a well pronounced spasticatonical state of the microcirculation bed vessels. On the fifth and seventh day the characteristic features were dilatation and decreased tonus of the venous part of the microcirculatory bed, redistribution of blood and more considerable disorders in microcirculation with aggregation and diapedesis of erythrocytes.", "contents": "[Character of changes in the microcirculatory bed of the mesentery of the small intestine in experimental acute myocardial infarct]. On the first day of the development of acute coronary insufficiency there appears a spastic state of all the vascular system of the small intestine mesentery and a deceleration of the blood flow. On the third day after ligation of the coronary artery branch against the background of spasmed arterioles the alternation of sites of constricted and dilated capillaries, postcapillaries and venules was observed, i.e., there was a well pronounced spasticatonical state of the microcirculation bed vessels. On the fifth and seventh day the characteristic features were dilatation and decreased tonus of the venous part of the microcirculatory bed, redistribution of blood and more considerable disorders in microcirculation with aggregation and diapedesis of erythrocytes.", "PMID": 962598}
{"id": "pubmed23n0032_5146", "title": "[Ultrastructural changes in the supraoptic nucleus and posterior lobe of the pituitary following experimental burns].", "content": "The dynamics of the ultrastructure of the neurosecretory system in the postburn period is characterized by phasic of organells of neurosecretory cells and synapses on their bodies and processes. Immediately after burn there appear pronounced signs of an increased formation of secretion synchronous to a considerably increased functional activity of synaptic structures. Of the opposite character are fine structures in neurocytes in the subsequent period (1-1,5 hour after burn). Phenomena of disintegration and degeneration of organells are developing in the cells. In the posiologically active neurohormones and degeneration of membrane structures gradually increase at all the observed stages of the burn trauma. All the elements of the supraoptic nucleus and the posterior lobe of the hypophysis early responding to the effect of such a powerful extreme factor are involved in the response of the organism to burn. It should be noted that immediately after burn there appears an increased permeability of capillaries followed by extravasation of formed elements and liquid blood. It steadily increases with the development of the process.", "contents": "[Ultrastructural changes in the supraoptic nucleus and posterior lobe of the pituitary following experimental burns]. The dynamics of the ultrastructure of the neurosecretory system in the postburn period is characterized by phasic of organells of neurosecretory cells and synapses on their bodies and processes. Immediately after burn there appear pronounced signs of an increased formation of secretion synchronous to a considerably increased functional activity of synaptic structures. Of the opposite character are fine structures in neurocytes in the subsequent period (1-1,5 hour after burn). Phenomena of disintegration and degeneration of organells are developing in the cells. In the posiologically active neurohormones and degeneration of membrane structures gradually increase at all the observed stages of the burn trauma. All the elements of the supraoptic nucleus and the posterior lobe of the hypophysis early responding to the effect of such a powerful extreme factor are involved in the response of the organism to burn. It should be noted that immediately after burn there appears an increased permeability of capillaries followed by extravasation of formed elements and liquid blood. It steadily increases with the development of the process.", "PMID": 962599}
{"id": "pubmed23n0032_5147", "title": "[Topography of the intraorganic veins of the pancreas].", "content": "The article describes the veins of the head, body and tail of the pancreas and the blood from them. Two ways of the blood outflow are found: 1.along the splenic and mesenteric veins and 2. along the splenic vein. The greatest amount of anastomoses is found in the area of transition from the head to the body. Three forms of the pancreatic venous bed are established: segmentary, reticular and mixed forms.", "contents": "[Topography of the intraorganic veins of the pancreas]. The article describes the veins of the head, body and tail of the pancreas and the blood from them. Two ways of the blood outflow are found: 1.along the splenic and mesenteric veins and 2. along the splenic vein. The greatest amount of anastomoses is found in the area of transition from the head to the body. Three forms of the pancreatic venous bed are established: segmentary, reticular and mixed forms.", "PMID": 962600}
{"id": "pubmed23n0032_5148", "title": "[Plasticity of the circulatory bed of the vermiform appendix in the presence of circulatory disorders within it].", "content": "The methods of macro-microscopy and angio-roentgenography were used to study the change of blood vessels of the vermiform process in 80 rabbits. Six series of experiments were performed with the exclusion of the appendicular artery and vein and their direct branches in different combinations. The normal circulatory bed was studied in 10 rabbits. It has been established that the circulatory bed of the rabbit vermiform process is characterized by a very complicated structure and has numerous intervascular connections. The latter possess high plasticity sustaining the sufficiently developed collateral circulation and the recovery of the blood inflow disturbed in the process. The work describes specific features of the process circulatory bed changes under the conditions of the experiments. The data obtained should be taken into account when modeling acute appendicitis in the rabbit by disturbing its vascularization.", "contents": "[Plasticity of the circulatory bed of the vermiform appendix in the presence of circulatory disorders within it]. The methods of macro-microscopy and angio-roentgenography were used to study the change of blood vessels of the vermiform process in 80 rabbits. Six series of experiments were performed with the exclusion of the appendicular artery and vein and their direct branches in different combinations. The normal circulatory bed was studied in 10 rabbits. It has been established that the circulatory bed of the rabbit vermiform process is characterized by a very complicated structure and has numerous intervascular connections. The latter possess high plasticity sustaining the sufficiently developed collateral circulation and the recovery of the blood inflow disturbed in the process. The work describes specific features of the process circulatory bed changes under the conditions of the experiments. The data obtained should be taken into account when modeling acute appendicitis in the rabbit by disturbing its vascularization.", "PMID": 962601}
{"id": "pubmed23n0032_5149", "title": "[Cytophotometric determination of DNA concentration in the nuclei of the human placental trophoblast in late toxemias of pregnancy].", "content": "The distribution of trophoblast nuclei of the human placenta by their ploidy was studied in normal pregnancy and in late pregnancy toxemias by the monowave emthod of cytophotometry in visible light. In the plasmodiotrophoblast a pronounced increase in the amount of nuclei in the S-phase (up to 40%) was noted in late toxemias which suggests a considerable activation of the DNA synthesis. The placenta cytotrophoblast in late toxemias is substantially different from that in normality. The Langhans cells disappear almost completely while in normality they are present in young terminal villi till the very end of pregnancy. The amount of the island cytotrophoblasts is much greater, the distribution of nuclei according t the DNA content being of absolutely different character than in normality. A great amount of hypodiploid nuclei (up to 36%) was observed which are absent in normal conditions as well as much greater fluctuations in the amount of diploid nuclei (from 4% to 37%) as compared with the normal amount (25.9%). These data show a high amitotic activity in the island cytotrophoblast in late toxemias. The above changes in the plasmodiotrophoblast and cytotrophoblast might be considered as a compensatory reaction of the placenta to a considerably increased vacuolization and deep atrophic processes in degenerating villi.", "contents": "[Cytophotometric determination of DNA concentration in the nuclei of the human placental trophoblast in late toxemias of pregnancy]. The distribution of trophoblast nuclei of the human placenta by their ploidy was studied in normal pregnancy and in late pregnancy toxemias by the monowave emthod of cytophotometry in visible light. In the plasmodiotrophoblast a pronounced increase in the amount of nuclei in the S-phase (up to 40%) was noted in late toxemias which suggests a considerable activation of the DNA synthesis. The placenta cytotrophoblast in late toxemias is substantially different from that in normality. The Langhans cells disappear almost completely while in normality they are present in young terminal villi till the very end of pregnancy. The amount of the island cytotrophoblasts is much greater, the distribution of nuclei according t the DNA content being of absolutely different character than in normality. A great amount of hypodiploid nuclei (up to 36%) was observed which are absent in normal conditions as well as much greater fluctuations in the amount of diploid nuclei (from 4% to 37%) as compared with the normal amount (25.9%). These data show a high amitotic activity in the island cytotrophoblast in late toxemias. The above changes in the plasmodiotrophoblast and cytotrophoblast might be considered as a compensatory reaction of the placenta to a considerably increased vacuolization and deep atrophic processes in degenerating villi.", "PMID": 962602}
{"id": "pubmed23n0032_5150", "title": "[Pathways of transcranial blood flow].", "content": "The injection of the injectional mass into the internal and external carotid arteries has shown the presence of perforant arteries in the bones of the cranial fornix. The method of impregnation of vessels by silver nitrate in sections of decalcified cranium bones enabled the author to establish the penetration of thin blood vessels into the cranial cavity through sutures. Blood vessels simultaneously found to be present in the spongy and compact substance of the cranial fornix bones. The totalities of the above vessels are considered as transcranial vascular communications. Their possible significance in hemodynamics is discussed.", "contents": "[Pathways of transcranial blood flow]. The injection of the injectional mass into the internal and external carotid arteries has shown the presence of perforant arteries in the bones of the cranial fornix. The method of impregnation of vessels by silver nitrate in sections of decalcified cranium bones enabled the author to establish the penetration of thin blood vessels into the cranial cavity through sutures. Blood vessels simultaneously found to be present in the spongy and compact substance of the cranial fornix bones. The totalities of the above vessels are considered as transcranial vascular communications. Their possible significance in hemodynamics is discussed.", "PMID": 962603}
{"id": "pubmed23n0032_5151", "title": "[Classification of neurons of the cat neocortex].", "content": "Different forms of neurons of the temporal cortex of the adult cat were studied in frontal and sagittal sections by the Golgi method in the author's modification. It was shown that the 2nd type Golgi cells (spider neurons, basket cells, spindle stellate cells with two bunches of processes, stellate cells with radial processes, Martinotti's cells) are characterized by local restruction of their processes and differ from one another in the specificity of axonal aborizations. The comparison of axonal systems of the pyramidal and above forms of the 2nd type Golgi cells has shown the set of collaterals of one pyramidal cell to consist of fragments of axonal branching of different forms of short-axon neurons. On the basis of the data obtained the pyramidal neuron is considered as a universal type of cell. The structural features of its receptive surface sustain the converging on it of a wide range of polymodal signals, the difference in the quality of presynaptic structures being responsible for the plasticity of a functional change of its integrative activity.", "contents": "[Classification of neurons of the cat neocortex]. Different forms of neurons of the temporal cortex of the adult cat were studied in frontal and sagittal sections by the Golgi method in the author's modification. It was shown that the 2nd type Golgi cells (spider neurons, basket cells, spindle stellate cells with two bunches of processes, stellate cells with radial processes, Martinotti's cells) are characterized by local restruction of their processes and differ from one another in the specificity of axonal aborizations. The comparison of axonal systems of the pyramidal and above forms of the 2nd type Golgi cells has shown the set of collaterals of one pyramidal cell to consist of fragments of axonal branching of different forms of short-axon neurons. On the basis of the data obtained the pyramidal neuron is considered as a universal type of cell. The structural features of its receptive surface sustain the converging on it of a wide range of polymodal signals, the difference in the quality of presynaptic structures being responsible for the plasticity of a functional change of its integrative activity.", "PMID": 962604}
{"id": "pubmed23n0032_5152", "title": "[Morphologic changes during neuroplastic nerve restoration].", "content": "The dynamics of ultrastructural changes in plastic recovery of the function of the additional nerve by the anterior branch of the second cervical nerve was studied. The nerve cells at the level of the donor-nerve were found to be highly reactive and plastic. It was established that in the process of heterogenic regeneration of the nerve the most substantial changes in neuronal structures were observed during the first two months. The cysterns of the endoplasmic network remained dilated for a long time after platic operation with might be related with the increased protein metabolism in the neuron.", "contents": "[Morphologic changes during neuroplastic nerve restoration]. The dynamics of ultrastructural changes in plastic recovery of the function of the additional nerve by the anterior branch of the second cervical nerve was studied. The nerve cells at the level of the donor-nerve were found to be highly reactive and plastic. It was established that in the process of heterogenic regeneration of the nerve the most substantial changes in neuronal structures were observed during the first two months. The cysterns of the endoplasmic network remained dilated for a long time after platic operation with might be related with the increased protein metabolism in the neuron.", "PMID": 962605}
{"id": "pubmed23n0032_5153", "title": "Familial myasthenia gravis. Report of four cases.", "content": "Two pairs of siblings with myasthenia gravis, belonging to two different families, are reported. This is the only record of familial myasthenia during the past twenty years, in a total of 145 patients seen at the Neurological Clinic of the S\u00e3o Paulo Medical School. In spite of the fact that myasthenia gravis does not show hereditary characteristics, the peculiar features of the four cases justify the present report. The two pairs of siblings were born from non myasthenic nor consanguineous parents. The disease started at birth showing bilateral partial eyelid ptosis in all patients. The course of the illness has been favorable. There was no thymoma.", "contents": "Familial myasthenia gravis. Report of four cases. Two pairs of siblings with myasthenia gravis, belonging to two different families, are reported. This is the only record of familial myasthenia during the past twenty years, in a total of 145 patients seen at the Neurological Clinic of the S\u00e3o Paulo Medical School. In spite of the fact that myasthenia gravis does not show hereditary characteristics, the peculiar features of the four cases justify the present report. The two pairs of siblings were born from non myasthenic nor consanguineous parents. The disease started at birth showing bilateral partial eyelid ptosis in all patients. The course of the illness has been favorable. There was no thymoma.", "PMID": 962633}
{"id": "pubmed23n0032_5154", "title": "[Polyneuropathy caused by tuberculostatics. Study of motor nerve conduction in 29 patients].", "content": "The electroneurographic study in 29 patients, during treatment with isoniazid for pulmonary tuberculosis in 12 months period is reported. The patients age were between 16 and 70 years. During the study period motor nerve conduction velocity was done every two months in the median, ulnar and peroneal nerves. The statistical analysis in a 6 months period, revealed p greater than 0.05 (nonsignificant) at the median and peroneal nerves, in spite of the lowering of the motor nerve conduction in a 12 months period. The same findings were observed with the ulnar nerve, but p less than 0.01 (significant). It was impossible to establish a correlation between the motor nerve conduction velocities and the clinical findings observed in the patients. This paper, in the author's opinion, shows that the nerve motor conduction technique is worthless in the follow up of isoniazid patients.", "contents": "[Polyneuropathy caused by tuberculostatics. Study of motor nerve conduction in 29 patients]. The electroneurographic study in 29 patients, during treatment with isoniazid for pulmonary tuberculosis in 12 months period is reported. The patients age were between 16 and 70 years. During the study period motor nerve conduction velocity was done every two months in the median, ulnar and peroneal nerves. The statistical analysis in a 6 months period, revealed p greater than 0.05 (nonsignificant) at the median and peroneal nerves, in spite of the lowering of the motor nerve conduction in a 12 months period. The same findings were observed with the ulnar nerve, but p less than 0.01 (significant). It was impossible to establish a correlation between the motor nerve conduction velocities and the clinical findings observed in the patients. This paper, in the author's opinion, shows that the nerve motor conduction technique is worthless in the follow up of isoniazid patients.", "PMID": 962634}
{"id": "pubmed23n0032_5155", "title": "[Decompression craniotomy in acute brain edema. Apropos of 30 operated cases].", "content": "Uni or bilateral decompressive craniotomy was carried out in 30 patients bearing severe cerebral edema of etiology predominantly traumatic. Coma, midriasis and decerebrate state were the mostly commonly clinical features presented. The average survival in the series was 46.6%.", "contents": "[Decompression craniotomy in acute brain edema. Apropos of 30 operated cases]. Uni or bilateral decompressive craniotomy was carried out in 30 patients bearing severe cerebral edema of etiology predominantly traumatic. Coma, midriasis and decerebrate state were the mostly commonly clinical features presented. The average survival in the series was 46.6%.", "PMID": 962635}
{"id": "pubmed23n0032_5156", "title": "[Value of cytogenetic study in transsexualism].", "content": "By reviewing the syndromic manifestation, transsexualism in characterized as a psychiatric entity, apart from homossexualism and transvestism. The two main feasible etiologic causes of transsexualism are discussed: the psychoanalytical hypothesis based upon psycossexual regression with imprinting of maternal figure and the neuroendocrine model which assumes alterations of the gender role identity centers in the hypothalamus. On the grounds of the latter explanation and after the scheme that seems to occure in the Morris syndrome whose cells (XY) do not respond to the masculinizing effect of plasma testosterone, it is proposed that transsexuals should possess detectable or cryptic sex-chromosome mosaicism affecting hypothalamic centers of gender role identity which do not respond to the androgenic secretion produced by primitive gonad. This possibliity explains the excessive prevalence of the syndrome among men, its typical features in the male as well as its sporadic occurrence. Cytogenetic investigation reveals that the frequency (32%) of sex-chromosome mosaicism among 25 transsexuals and 40 normal control people, both groups presenting the proportion of the mosaicism practically null. The karyotypic criterion as a valuable aid in the syndrome diagnosis is considered.", "contents": "[Value of cytogenetic study in transsexualism]. By reviewing the syndromic manifestation, transsexualism in characterized as a psychiatric entity, apart from homossexualism and transvestism. The two main feasible etiologic causes of transsexualism are discussed: the psychoanalytical hypothesis based upon psycossexual regression with imprinting of maternal figure and the neuroendocrine model which assumes alterations of the gender role identity centers in the hypothalamus. On the grounds of the latter explanation and after the scheme that seems to occure in the Morris syndrome whose cells (XY) do not respond to the masculinizing effect of plasma testosterone, it is proposed that transsexuals should possess detectable or cryptic sex-chromosome mosaicism affecting hypothalamic centers of gender role identity which do not respond to the androgenic secretion produced by primitive gonad. This possibliity explains the excessive prevalence of the syndrome among men, its typical features in the male as well as its sporadic occurrence. Cytogenetic investigation reveals that the frequency (32%) of sex-chromosome mosaicism among 25 transsexuals and 40 normal control people, both groups presenting the proportion of the mosaicism practically null. The karyotypic criterion as a valuable aid in the syndrome diagnosis is considered.", "PMID": 962636}
{"id": "pubmed23n0032_5157", "title": "[Ciliospinal reflex in man].", "content": "The study of the ciliospinal reflex in twenty-three patients is reported. The patients were divided into two groups: the first with spinal cord lesions and the second one with brain stem lesions. The authors discuss the pathways and the mechanisms of the ciliospinal reflex in man and emphasize their value on the diagnosis of brain stem lesions.", "contents": "[Ciliospinal reflex in man]. The study of the ciliospinal reflex in twenty-three patients is reported. The patients were divided into two groups: the first with spinal cord lesions and the second one with brain stem lesions. The authors discuss the pathways and the mechanisms of the ciliospinal reflex in man and emphasize their value on the diagnosis of brain stem lesions.", "PMID": 962637}
{"id": "pubmed23n0032_5158", "title": "[Cerebrovascular diseases of the \"moyamoya\" type].", "content": "The case of a 34-year-old caucasian male with subarachnoid hemorrahge is reported. The right carotid arteriogram shows the typical internal carotid artery occlusion at the syphon, whith an abnormal vascular network at the base of the brain; the right anterior and middle cerebral arteries fill from the vascular network and through meningocortical anastomoses; the right posterior communicating and cerebral arteries are tortuous and hypertrophic. The left carotid arteriogram shows hypertrophy of the common and internal carotid arteries; both anterior and middle cerebral arteries fill from this side; the anterior communicating artery is thick (foetal type) and a median anterior cerebral artery to the corpus callosum is present. It is the authors' view that the \"moyamoya\" disease is a congenital one, since the abnormal vascular network at the base of the brain would not be necessary, in this case, to preserve good collateral circulation to the right cerebral hemisphere. No other case with the typical findings of \"moyamoya\" disease on one side and hypertrophy of the carotid arteries on the other was found in the literature.", "contents": "[Cerebrovascular diseases of the \"moyamoya\" type]. The case of a 34-year-old caucasian male with subarachnoid hemorrahge is reported. The right carotid arteriogram shows the typical internal carotid artery occlusion at the syphon, whith an abnormal vascular network at the base of the brain; the right anterior and middle cerebral arteries fill from the vascular network and through meningocortical anastomoses; the right posterior communicating and cerebral arteries are tortuous and hypertrophic. The left carotid arteriogram shows hypertrophy of the common and internal carotid arteries; both anterior and middle cerebral arteries fill from this side; the anterior communicating artery is thick (foetal type) and a median anterior cerebral artery to the corpus callosum is present. It is the authors' view that the \"moyamoya\" disease is a congenital one, since the abnormal vascular network at the base of the brain would not be necessary, in this case, to preserve good collateral circulation to the right cerebral hemisphere. No other case with the typical findings of \"moyamoya\" disease on one side and hypertrophy of the carotid arteries on the other was found in the literature.", "PMID": 962638}
{"id": "pubmed23n0032_5159", "title": "[Carcinoma of the choroid plexus. Report of a case].", "content": "The case of a three-years-old male with intracranial hypertension is reported. Without a clear clinical diagnosis, a gradual deterioration ocurred before his death, 8 days later. The neuropathological examination of the brain revealed a tumor with indistint borders at the right lateral ventricle, with granulous surface, pink-gray colour, irregular necrotic and hemorragic areas, invading the adjacent brain tissue. The histopathological study revealed a malignant variant of the choroid plexus tumor, of extreme rarity in the central nervous system. The biological and histological criteria accepted by Lewis, Rubinstein and Polak for the correct diagnosis of this type of tumour are discussed.", "contents": "[Carcinoma of the choroid plexus. Report of a case]. The case of a three-years-old male with intracranial hypertension is reported. Without a clear clinical diagnosis, a gradual deterioration ocurred before his death, 8 days later. The neuropathological examination of the brain revealed a tumor with indistint borders at the right lateral ventricle, with granulous surface, pink-gray colour, irregular necrotic and hemorragic areas, invading the adjacent brain tissue. The histopathological study revealed a malignant variant of the choroid plexus tumor, of extreme rarity in the central nervous system. The biological and histological criteria accepted by Lewis, Rubinstein and Polak for the correct diagnosis of this type of tumour are discussed.", "PMID": 962639}
{"id": "pubmed23n0032_5160", "title": "[Metastatic carcinoma involving the cauda equina].", "content": "The case of a 60 year-old man who had an indifferenciated carcinoma in the lung is reported. He had received cobaltotherapy and had improved. After 3 months a lumbar pain had began and the patient was hospitalized. A myelography with lipiodol demonstrated and intra-dural mass. At operation a big intra-dural tumor including the cauda equina was found. The microscopic examination revealed an undifferenciated carcinoma, that inflitrated the epi and peri-nerual space.", "contents": "[Metastatic carcinoma involving the cauda equina]. The case of a 60 year-old man who had an indifferenciated carcinoma in the lung is reported. He had received cobaltotherapy and had improved. After 3 months a lumbar pain had began and the patient was hospitalized. A myelography with lipiodol demonstrated and intra-dural mass. At operation a big intra-dural tumor including the cauda equina was found. The microscopic examination revealed an undifferenciated carcinoma, that inflitrated the epi and peri-nerual space.", "PMID": 962640}
{"id": "pubmed23n0032_5161", "title": "[Clinico genetic discussion on Apert's syndrome. Apropos of a case].", "content": "After a short introduction about the so-called Apert's syndrome. a clinical suggestive case is reported. Discussion shows how a clinic-genetical procedure may help the clinicians.", "contents": "[Clinico genetic discussion on Apert's syndrome. Apropos of a case]. After a short introduction about the so-called Apert's syndrome. a clinical suggestive case is reported. Discussion shows how a clinic-genetical procedure may help the clinicians.", "PMID": 962641}
{"id": "pubmed23n0032_5162", "title": "[Pituitary apoplexy after pneumoencefalogram].", "content": "A case of pituitary apoplexy following the performance of a pneumoencephalogram is reported. No such a case has been published in the literature, to the author's knowledge. The etiopathogenetic mechanism is discussed and a recomendation is made that surgery should be performed straight after completion of the pneumoencephalogram, should it show a significant supraselar extension of a pituitary adenoma, even in the absence of increased intracranial pressure.", "contents": "[Pituitary apoplexy after pneumoencefalogram]. A case of pituitary apoplexy following the performance of a pneumoencephalogram is reported. No such a case has been published in the literature, to the author's knowledge. The etiopathogenetic mechanism is discussed and a recomendation is made that surgery should be performed straight after completion of the pneumoencephalogram, should it show a significant supraselar extension of a pituitary adenoma, even in the absence of increased intracranial pressure.", "PMID": 962642}
{"id": "pubmed23n0032_5163", "title": "Effects of nerve stimulation on human muscle.", "content": "A phasic program of femoral nerve stimulation was used  to reduce refractory knee flexion contractures in five patients. In one, rectus femoris was tenotomized before starting stimulation to reduce hip flexion contracture. Muscle biopsies were performed before and after 5 to 12 weeks of intermittent stimulation  at separated sites on the same muscle. In the four patients where muscle contraction was isometric, type I fibers increased 3.7%, 6.4%, 48.4%, anand 30.4%, respectively. Both fiber types in each patient showed an increase in size ( p less than .001). In the tenotomized muscle, where contraction was isotonic at a shortened length, the proportion of type I fibers decreased from 40.2% to 25.4%, and their mean diameter also decreased (p less than .001). These observations suggest that the physical conditions of contraction may be more important than the pattern of neuronal discharge in determining the metabolic profile of human muscle fibers.", "contents": "Effects of nerve stimulation on human muscle. A phasic program of femoral nerve stimulation was used  to reduce refractory knee flexion contractures in five patients. In one, rectus femoris was tenotomized before starting stimulation to reduce hip flexion contracture. Muscle biopsies were performed before and after 5 to 12 weeks of intermittent stimulation  at separated sites on the same muscle. In the four patients where muscle contraction was isometric, type I fibers increased 3.7%, 6.4%, 48.4%, anand 30.4%, respectively. Both fiber types in each patient showed an increase in size ( p less than .001). In the tenotomized muscle, where contraction was isotonic at a shortened length, the proportion of type I fibers decreased from 40.2% to 25.4%, and their mean diameter also decreased (p less than .001). These observations suggest that the physical conditions of contraction may be more important than the pattern of neuronal discharge in determining the metabolic profile of human muscle fibers.", "PMID": 962643}
{"id": "pubmed23n0032_5164", "title": "Cervical spondylotic radiculopathy and myelopathy. A long-term follow-up study.", "content": "The natural history of cervical spondylotic myelopathy in a series of 55 patients resulted in a moderate to severe disability during a mean period of 45 months. Results of operation for myelopathy indicated improvement in one group that was maintained for a mean of 85 months. In another group, progressive worsening occurred after operation, or late worsening occurred, in some instances as long as 8 to 12 years after improvement and plateau. Patients with cervical spondylotic radiculopathy tended to be separated from those with myelopathy with respect to presentation, symptom complex, and operative result. Results of operation for radiculopathy were consistently good. A worsened disability postoperatively for patients with cervical spondylotic myelopathy was associated with the preoperative symptom of sphincter disturbance and the sign of lower extremity weakness. Change in hand movement after operation for myelopathy and change in distance walking ability were not correlated with numerous preoperative factors. A trend of improvement in disability following anterior interbody fusion and a tendency to worsen in disability following all varities of laminectomy were significant.", "contents": "Cervical spondylotic radiculopathy and myelopathy. A long-term follow-up study. The natural history of cervical spondylotic myelopathy in a series of 55 patients resulted in a moderate to severe disability during a mean period of 45 months. Results of operation for myelopathy indicated improvement in one group that was maintained for a mean of 85 months. In another group, progressive worsening occurred after operation, or late worsening occurred, in some instances as long as 8 to 12 years after improvement and plateau. Patients with cervical spondylotic radiculopathy tended to be separated from those with myelopathy with respect to presentation, symptom complex, and operative result. Results of operation for radiculopathy were consistently good. A worsened disability postoperatively for patients with cervical spondylotic myelopathy was associated with the preoperative symptom of sphincter disturbance and the sign of lower extremity weakness. Change in hand movement after operation for myelopathy and change in distance walking ability were not correlated with numerous preoperative factors. A trend of improvement in disability following anterior interbody fusion and a tendency to worsen in disability following all varities of laminectomy were significant.", "PMID": 962644}
{"id": "pubmed23n0032_5165", "title": "Anticonvulsant drug mechanisms. Phenytoin, phenobarbital, and ethosuximide and calcium flux in isolated presynaptic endings.", "content": "Phenytoin, phenobarbital, ethosuximide, and procaine hydrochloride were evaluated for their ability to inhibit Ca2+ flux into isolated presynaptic endings (synaptosomes) prepared from rabbit neocortex. Calcium influx produced by depolarizing concentrations (69 mM) of K+ was inhibited 7% to 63% by phenytoin, phenobarbital, or procaine, whereas ethosuximide was ineffective. Decreased Ca2+ influx was observed with as little as 0.08 mM phenytoin and 0.04 mM phenobarbital. In contrast, 4 mM procaine was needed to produce an effect. These results lead to the conclusion that ability to produce membrane stabilization is not a property of all anticonvulsant drugs; however, this property may be essential for the action of drugs effective in the treatment of major seizures.", "contents": "Anticonvulsant drug mechanisms. Phenytoin, phenobarbital, and ethosuximide and calcium flux in isolated presynaptic endings. Phenytoin, phenobarbital, ethosuximide, and procaine hydrochloride were evaluated for their ability to inhibit Ca2+ flux into isolated presynaptic endings (synaptosomes) prepared from rabbit neocortex. Calcium influx produced by depolarizing concentrations (69 mM) of K+ was inhibited 7% to 63% by phenytoin, phenobarbital, or procaine, whereas ethosuximide was ineffective. Decreased Ca2+ influx was observed with as little as 0.08 mM phenytoin and 0.04 mM phenobarbital. In contrast, 4 mM procaine was needed to produce an effect. These results lead to the conclusion that ability to produce membrane stabilization is not a property of all anticonvulsant drugs; however, this property may be essential for the action of drugs effective in the treatment of major seizures.", "PMID": 962645}
{"id": "pubmed23n0032_5166", "title": "Regeneration in Duchenne muscular dystrophy. Electromyographic evidence.", "content": "Eight patients with Duchenne muscular dystrophy (DMD) and seven normal children of similar age were studied with a new electromyographic method for coherent displays of potentials. Five hundred motor unit potentials (MUPs) were analyzed in 20 proximal and distal muscles representing a wide spectrum of dystrophy. The progressive MUP disintegration by dropping out of muscle fibers was documented, as well as a high incidence of spontaneous fibrillation. A total of 386 late component (LC) potentials followed the 500 MUPs at consistent latencies. No LC occurred in normal children. The LCs result from motor axon sprouts innervating muscle fibers that are newly formed either by segmentation of existing muscle fibers (focal neucrosis and membrane repair) or by muscle regeneration. Axons in DMD can thus collaterally innervate additional muscle fibers. These processes must delay the onset and progression of clinical weakness in DMD patients.", "contents": "Regeneration in Duchenne muscular dystrophy. Electromyographic evidence. Eight patients with Duchenne muscular dystrophy (DMD) and seven normal children of similar age were studied with a new electromyographic method for coherent displays of potentials. Five hundred motor unit potentials (MUPs) were analyzed in 20 proximal and distal muscles representing a wide spectrum of dystrophy. The progressive MUP disintegration by dropping out of muscle fibers was documented, as well as a high incidence of spontaneous fibrillation. A total of 386 late component (LC) potentials followed the 500 MUPs at consistent latencies. No LC occurred in normal children. The LCs result from motor axon sprouts innervating muscle fibers that are newly formed either by segmentation of existing muscle fibers (focal neucrosis and membrane repair) or by muscle regeneration. Axons in DMD can thus collaterally innervate additional muscle fibers. These processes must delay the onset and progression of clinical weakness in DMD patients.", "PMID": 962646}
{"id": "pubmed23n0032_5167", "title": "Clinical vs quantitative evaluation of cutaneous sensation.", "content": "A comparison of the evaluation of cutaneous sensation by neurologic examination and by quantitative assessment in 107 patients with various neuromuscular disease has shown that there is a reasonably good correlation between the recognition of abnormalities of cutaneous sensation by clinical and by quantitative methods. Clinical neurologists tend to underestimate abnormalities of touch-pressure sensation. The quantitative evaluation of cutaneous sensation is useful in quality control of the clinical examination, in correlating the nature of sensation loss with the compound action potential and morphometry of biopsied nerve, in providing a score of sensation to follow the course and the effect of treatment protocols, and in monitoring for an adverse effect on sensation by an environmental or industrial poison. There is a good correlation between abnormality of touch-pressure sensation and loss of large myelinated fibers of sural nerve in neuropathy.", "contents": "Clinical vs quantitative evaluation of cutaneous sensation. A comparison of the evaluation of cutaneous sensation by neurologic examination and by quantitative assessment in 107 patients with various neuromuscular disease has shown that there is a reasonably good correlation between the recognition of abnormalities of cutaneous sensation by clinical and by quantitative methods. Clinical neurologists tend to underestimate abnormalities of touch-pressure sensation. The quantitative evaluation of cutaneous sensation is useful in quality control of the clinical examination, in correlating the nature of sensation loss with the compound action potential and morphometry of biopsied nerve, in providing a score of sensation to follow the course and the effect of treatment protocols, and in monitoring for an adverse effect on sensation by an environmental or industrial poison. There is a good correlation between abnormality of touch-pressure sensation and loss of large myelinated fibers of sural nerve in neuropathy.", "PMID": 962647}
{"id": "pubmed23n0032_5168", "title": "Serum dopamine-beta-hydroxylase activity in migraine.", "content": "Serum dopamine-beta-hydroxylase (DBH) activity was measured during headache-free intervals in 17 patients with migraine and during the headache interval in 16 patients with muscle contraction headache, as well as in 40 normal subjects. The DBH activity was significantly higher in the migraine patients (46.5+/4.5 units) than in the controls (24.9+/2.4 units), whereas no significant difference was observed between the patients with muscle contraction headache (29.4+/4.5 units) and the controls.", "contents": "Serum dopamine-beta-hydroxylase activity in migraine. Serum dopamine-beta-hydroxylase (DBH) activity was measured during headache-free intervals in 17 patients with migraine and during the headache interval in 16 patients with muscle contraction headache, as well as in 40 normal subjects. The DBH activity was significantly higher in the migraine patients (46.5+/4.5 units) than in the controls (24.9+/2.4 units), whereas no significant difference was observed between the patients with muscle contraction headache (29.4+/4.5 units) and the controls.", "PMID": 962648}
{"id": "pubmed23n0032_5169", "title": "Cerebellar degeneration with Hodgkin disease. An immunological study.", "content": "A 21-year-old woman with subacute cerebellar degeneration was found to have Hodgkin lymphoma. Radiation therapy for the lymphoma halted the progression of her neurological disease. Using an immunofluorescent technique, we found the patient's serum to have antibodies to cerebellar Purkinje cells.", "contents": "Cerebellar degeneration with Hodgkin disease. An immunological study. A 21-year-old woman with subacute cerebellar degeneration was found to have Hodgkin lymphoma. Radiation therapy for the lymphoma halted the progression of her neurological disease. Using an immunofluorescent technique, we found the patient's serum to have antibodies to cerebellar Purkinje cells.", "PMID": 962649}
{"id": "pubmed23n0032_5170", "title": "Rapid eye movements in myasthenia gravis. II. Electro-oculographic analysis.", "content": "Voluntary saccades were studied by electro-oculography in ten patients with myasthenia gravis (MG)  and in eight patients with other types of ophthalmoplegia. Despite limited range of eye movements, maximum velocities of 20 degree and 40 degree saccades in patients with MG were not significantly different from  those in normal individuals, whereas maximum velocities in patients with other types of ophthalmoplegia were significantly decreased. In some myasthenic patients, small amplitude saccades were hypermetric and had high velocities, appearing clinically as \"quiver\" movements characteristic of MG. In MG the preservation of saccades with high initial velocities, even in the presence of severe ophthalmoplegia, suggests that muscle fibers generating rapid movements during saccades (twitch fibers) can be relatively spared when muscle fibers responsible for maintenance of excentric gaze (tonic fibers) are severely affected.", "contents": "Rapid eye movements in myasthenia gravis. II. Electro-oculographic analysis. Voluntary saccades were studied by electro-oculography in ten patients with myasthenia gravis (MG)  and in eight patients with other types of ophthalmoplegia. Despite limited range of eye movements, maximum velocities of 20 degree and 40 degree saccades in patients with MG were not significantly different from  those in normal individuals, whereas maximum velocities in patients with other types of ophthalmoplegia were significantly decreased. In some myasthenic patients, small amplitude saccades were hypermetric and had high velocities, appearing clinically as \"quiver\" movements characteristic of MG. In MG the preservation of saccades with high initial velocities, even in the presence of severe ophthalmoplegia, suggests that muscle fibers generating rapid movements during saccades (twitch fibers) can be relatively spared when muscle fibers responsible for maintenance of excentric gaze (tonic fibers) are severely affected.", "PMID": 962656}
{"id": "pubmed23n0032_5171", "title": "Limits of constriction in the treatment of retinal detachment.", "content": "A constricting silicone band was added to 13% of local sponge buckle operations in this series. The purpose was to relieve circumferential traction or to maintain a high buckle. The inclusion of a cerclage did not require drainage of subretinal fluid. The rate of reattachment without drainage (42 of 50 detachments) is comparable to what might be expected if the subretinal fluid had been drained. Constriction was controlled and limited to between 10% and 25% of the circumference of the eye. Anterior segment  ischemia requiring relaxation of the band occurred in two patients; scleral erosion did not occur.", "contents": "Limits of constriction in the treatment of retinal detachment. A constricting silicone band was added to 13% of local sponge buckle operations in this series. The purpose was to relieve circumferential traction or to maintain a high buckle. The inclusion of a cerclage did not require drainage of subretinal fluid. The rate of reattachment without drainage (42 of 50 detachments) is comparable to what might be expected if the subretinal fluid had been drained. Constriction was controlled and limited to between 10% and 25% of the circumference of the eye. Anterior segment  ischemia requiring relaxation of the band occurred in two patients; scleral erosion did not occur.", "PMID": 962657}
{"id": "pubmed23n0032_5172", "title": "Hereditary congenital alacrima.", "content": "We describe a family with markedly deficient lacrimation from infancy and punctate corneal epithelial erosions. An autosomal dominant inheritance pattern is suggested. A hypoplasia of the lacrimal gland in this family was suggested by pharmacologic testing and by histopathologic examination of the lacrimal gland of the proposita. The family investigated in this report represents the first instance of hereditary congenital alacrima without associated ocular or adnexal abnormalities and apart from systemic disorders such as the Riley-Day syndrome and anhidrotic ectodermal dysplasia.", "contents": "Hereditary congenital alacrima. We describe a family with markedly deficient lacrimation from infancy and punctate corneal epithelial erosions. An autosomal dominant inheritance pattern is suggested. A hypoplasia of the lacrimal gland in this family was suggested by pharmacologic testing and by histopathologic examination of the lacrimal gland of the proposita. The family investigated in this report represents the first instance of hereditary congenital alacrima without associated ocular or adnexal abnormalities and apart from systemic disorders such as the Riley-Day syndrome and anhidrotic ectodermal dysplasia.", "PMID": 962658}
{"id": "pubmed23n0032_5173", "title": "Surgical decisions in chronic angle-closure glaucoma.", "content": "A retrospective study of 57 eyes with chronic angle-closure showed that eyes with visual field loss are less likely to be improved by iridectomy than those without visual field loss in the presence of visual field loss, trabeculectomy achieved more satisfactory control of glaucoma than did iridectomy, and there was no noticeable difference in surgical complications between iridectomy and trabeculectomy in this small series. It is suggested that iridectomy is indicated for most cases of chronic angle closure without visual field loss, especially if the pressure is medically controllable. In the presence of visual field loss, iridectomy is usually a wise choice if medical control is easily achieved preoperatively. However, trabeculectomy might be the best choice in most patients with visual field loss and medically uncontrolled pressure, regardless of the gonioscopic findings.", "contents": "Surgical decisions in chronic angle-closure glaucoma. A retrospective study of 57 eyes with chronic angle-closure showed that eyes with visual field loss are less likely to be improved by iridectomy than those without visual field loss in the presence of visual field loss, trabeculectomy achieved more satisfactory control of glaucoma than did iridectomy, and there was no noticeable difference in surgical complications between iridectomy and trabeculectomy in this small series. It is suggested that iridectomy is indicated for most cases of chronic angle closure without visual field loss, especially if the pressure is medically controllable. In the presence of visual field loss, iridectomy is usually a wise choice if medical control is easily achieved preoperatively. However, trabeculectomy might be the best choice in most patients with visual field loss and medically uncontrolled pressure, regardless of the gonioscopic findings.", "PMID": 962659}
{"id": "pubmed23n0032_5174", "title": "Ocular Ehlers-Danlos syndrome with normal lysyl hydroxylase activity.", "content": "We report two brothers affected with what has been called either fragilitas oculi or the Ehlers-Danlos syndrome type VI. Previously reported cases of the Ehlers-Danlos syndrome type VI showed a deficiency of lysyl hydroxylase in cultured fibroblasts. Assays of cultured skin fibroblasts from these two boys yielded normal activity of this enzyme, suggesting that there are two variants of this disease.", "contents": "Ocular Ehlers-Danlos syndrome with normal lysyl hydroxylase activity. We report two brothers affected with what has been called either fragilitas oculi or the Ehlers-Danlos syndrome type VI. Previously reported cases of the Ehlers-Danlos syndrome type VI showed a deficiency of lysyl hydroxylase in cultured fibroblasts. Assays of cultured skin fibroblasts from these two boys yielded normal activity of this enzyme, suggesting that there are two variants of this disease.", "PMID": 962660}
{"id": "pubmed23n0032_5175", "title": "Color perimetry.", "content": "Seven subjects were studied to determine the reproducibility of color isopters utilizing a Tubingen perimeter with targets equated for radiant energy and separate for heterochromatic flicker luminance. Achromatic threshold recognition of targets for equal luminance gave smaller isopters with longer wavelengths (red). Color recognition thresholds, on the other hand, showed large blue, midzone red and green, and small yellow isopters. The target recognition and color recognition thresholds for equal energy targets gave smaller red isopters. The data support Traquair's contention that all color isopters would be equivalent if hue, saturation, and intensity were equated. Clinically, the detection of subtle peripheral and central field defects might reside in the use of appropriately selected equally bright-colored targets.", "contents": "Color perimetry. Seven subjects were studied to determine the reproducibility of color isopters utilizing a Tubingen perimeter with targets equated for radiant energy and separate for heterochromatic flicker luminance. Achromatic threshold recognition of targets for equal luminance gave smaller isopters with longer wavelengths (red). Color recognition thresholds, on the other hand, showed large blue, midzone red and green, and small yellow isopters. The target recognition and color recognition thresholds for equal energy targets gave smaller red isopters. The data support Traquair's contention that all color isopters would be equivalent if hue, saturation, and intensity were equated. Clinically, the detection of subtle peripheral and central field defects might reside in the use of appropriately selected equally bright-colored targets.", "PMID": 962661}
{"id": "pubmed23n0032_5176", "title": "Subretinal tracks in ophthalmomyiasis.", "content": "Three cases of presumed infestation of the human globe by the maggot of a true fly (order of Diptera) are presented. In each case, migration of the maggot through the subretinal space produced widespread ophthalmoscopic and fluorescein angiographic changes that are believed to be pathognomonic of subretinal ophthalmomyiasis. Despite extensive  changes in the retinal pigment epithelium, each of the three patients expressed few symptoms and manifested minimal visual deficit.", "contents": "Subretinal tracks in ophthalmomyiasis. Three cases of presumed infestation of the human globe by the maggot of a true fly (order of Diptera) are presented. In each case, migration of the maggot through the subretinal space produced widespread ophthalmoscopic and fluorescein angiographic changes that are believed to be pathognomonic of subretinal ophthalmomyiasis. Despite extensive  changes in the retinal pigment epithelium, each of the three patients expressed few symptoms and manifested minimal visual deficit.", "PMID": 962662}
{"id": "pubmed23n0032_5177", "title": "Sebaceous gland carcinoma of the eyelid.", "content": "A 37-year old woman had received multiple radiation treatments between the ages of 6 months and 15 years for an extensive cavernous hemangioma of the left side of the face. At the age of 27 years, a biopsy specimen from a lesion of the left buccal commissure disclosed  squamous cell carcinoma arising in chronic radiodermatitis. Multiple cutaneous lesions were excised surgically, and extensive skin grafting of the left side of the face was performed. At the age of 35, she developed a tumor of the left upper and lower eyelids that extended into the orbit, which proved to be a sebaceous gland carcinoma. Following left orbital exenteration, the tumor promptly recurred in the socket. The patient died with widespread metastatic lesions nine months after exenteration. This case, which, to our knowledge, represents the fourth example of postradiation sebaceous gland carcinoma of the eyelids, differs from the previously reported cases in that the radiation therapy had been given for a benign cutaneous condition.", "contents": "Sebaceous gland carcinoma of the eyelid. A 37-year old woman had received multiple radiation treatments between the ages of 6 months and 15 years for an extensive cavernous hemangioma of the left side of the face. At the age of 27 years, a biopsy specimen from a lesion of the left buccal commissure disclosed  squamous cell carcinoma arising in chronic radiodermatitis. Multiple cutaneous lesions were excised surgically, and extensive skin grafting of the left side of the face was performed. At the age of 35, she developed a tumor of the left upper and lower eyelids that extended into the orbit, which proved to be a sebaceous gland carcinoma. Following left orbital exenteration, the tumor promptly recurred in the socket. The patient died with widespread metastatic lesions nine months after exenteration. This case, which, to our knowledge, represents the fourth example of postradiation sebaceous gland carcinoma of the eyelids, differs from the previously reported cases in that the radiation therapy had been given for a benign cutaneous condition.", "PMID": 962663}
{"id": "pubmed23n0032_5178", "title": "Further experience with the pinch technique for repair of eyelid deformities.", "content": "The pinch technique has been found to be useful in repairing cosmetic eyelid deformities. However, the local anesthetic containing hyaluronidase must be injected only in small amounts and only into the subcutaneous space. Scar tissue and skin that is firmly adherent to underlying muscle do not yield a satisfactory ridge, and therefore, the pinch technique should not be used. Ectropion can be predicted by the observation of eversion of the lid margin when even only minimal skin is pinched, and impending ectropion can be discovered by our \"lean forward and look up\" maneuver. An ectropion repair can then be combined with the blepharoplasty surgical operation. The pinch technique has also been found useful when upper and lower blepharoplasties are joined laterally to elevate the lateral canthus and eliminate \"crow's feet.\" One component of a repair of trichiasis also involves the use of the pinch technique.", "contents": "Further experience with the pinch technique for repair of eyelid deformities. The pinch technique has been found to be useful in repairing cosmetic eyelid deformities. However, the local anesthetic containing hyaluronidase must be injected only in small amounts and only into the subcutaneous space. Scar tissue and skin that is firmly adherent to underlying muscle do not yield a satisfactory ridge, and therefore, the pinch technique should not be used. Ectropion can be predicted by the observation of eversion of the lid margin when even only minimal skin is pinched, and impending ectropion can be discovered by our \"lean forward and look up\" maneuver. An ectropion repair can then be combined with the blepharoplasty surgical operation. The pinch technique has also been found useful when upper and lower blepharoplasties are joined laterally to elevate the lateral canthus and eliminate \"crow's feet.\" One component of a repair of trichiasis also involves the use of the pinch technique.", "PMID": 962664}
{"id": "pubmed23n0032_5179", "title": "Vitreous membranes. A light and electron microscopical study.", "content": "Patients with pigmented vitreous membranes were subjected to partial vitrectomy, and the membranes were studied by light and electron microscopy. Two yellow ochre membranes from eyes removed at autopsy were also examined. The membranes were  composed of degenerating red cells and a delicate matrix of collagen. Phagocytic cells of uncertain origin and migrating retinal pigment epithelial cells were present in some of the membranes. Vascular channels were observed in only two cases, in which the membranes had clinical features different from those in the remaining cases. The development of new techniques of vitrectomy and the relatively normal clinical appearance of the posterior pole of the retina in these cases both suggest the potential clinical value of vitreous membrane excision. Additional histopathologic studies of vitrectomy specimens will enhance our understanding of the indications for this procedure.", "contents": "Vitreous membranes. A light and electron microscopical study. Patients with pigmented vitreous membranes were subjected to partial vitrectomy, and the membranes were studied by light and electron microscopy. Two yellow ochre membranes from eyes removed at autopsy were also examined. The membranes were  composed of degenerating red cells and a delicate matrix of collagen. Phagocytic cells of uncertain origin and migrating retinal pigment epithelial cells were present in some of the membranes. Vascular channels were observed in only two cases, in which the membranes had clinical features different from those in the remaining cases. The development of new techniques of vitrectomy and the relatively normal clinical appearance of the posterior pole of the retina in these cases both suggest the potential clinical value of vitreous membrane excision. Additional histopathologic studies of vitrectomy specimens will enhance our understanding of the indications for this procedure.", "PMID": 962665}
{"id": "pubmed23n0032_5180", "title": "Aqueous ascorbate concentration in hereditary buphthalmic rabbits.", "content": "Some  aqueous humor constituents, including ascorbate, protein, and sodium, potassium, and calcium ions, were analyzed in three groups of adult buphthalmic (bu/bu) rabbits from strain AXBU/J and in control rabbits of the closely related strain AX/J. Aqueous humor ascorbate concentration of the normal strain was 22.5/\"2.3mg/100ml. The buphthalmic rabbits that had no meaningful clinical signs of glaucoma at the time of sample collection had lower values (11.7/\"1.7mg/100ml). Much lower values were observed in the bu/bu mutant rabbits that showed mild to severe glaucoma: 8.9/\"3.8mg/100 ml and 1.9\"/0.4mg/100ml, respectively. It was concluded that reduction of aqueous ascorbate is a biochemical  change associated with the pathological development of buphthalmia in rabbits of the AXBU/J strain. However, the exact mechanism is still unclear.", "contents": "Aqueous ascorbate concentration in hereditary buphthalmic rabbits. Some  aqueous humor constituents, including ascorbate, protein, and sodium, potassium, and calcium ions, were analyzed in three groups of adult buphthalmic (bu/bu) rabbits from strain AXBU/J and in control rabbits of the closely related strain AX/J. Aqueous humor ascorbate concentration of the normal strain was 22.5/\"2.3mg/100ml. The buphthalmic rabbits that had no meaningful clinical signs of glaucoma at the time of sample collection had lower values (11.7/\"1.7mg/100ml). Much lower values were observed in the bu/bu mutant rabbits that showed mild to severe glaucoma: 8.9/\"3.8mg/100 ml and 1.9\"/0.4mg/100ml, respectively. It was concluded that reduction of aqueous ascorbate is a biochemical  change associated with the pathological development of buphthalmia in rabbits of the AXBU/J strain. However, the exact mechanism is still unclear.", "PMID": 962666}
{"id": "pubmed23n0032_5181", "title": "Electron microscopical study of the Fleisher ring.", "content": "The  Fleischer ring of keratoconus was studied with the transmission electron microscope in four corneal buttons. The ring was characterized by accumulations of ferritin particles in the widened intercellular spaces and/or in the cytoplasmic vacuoles of the corneal epithelium. Both changes were prominent in basal layers in three cases; in one case, ferritin-containing vacuoles were noted in wing cell layers. Ferritin particles were also scattered over the corneal epithelium in all cases. For comparison, normal human corneas and conjunctivas were studied. Ferritin particles were scattered over the corneal epithelium and throughout the basal cells of the conjunctiva. They were not found in corneal stroma or endothelium. In conjunctival stroma, numerous ferritin particles were observed in the cytoplasm of some macrophages. Possible origin of these particles and the cause of their deposition are discussed.", "contents": "Electron microscopical study of the Fleisher ring. The  Fleischer ring of keratoconus was studied with the transmission electron microscope in four corneal buttons. The ring was characterized by accumulations of ferritin particles in the widened intercellular spaces and/or in the cytoplasmic vacuoles of the corneal epithelium. Both changes were prominent in basal layers in three cases; in one case, ferritin-containing vacuoles were noted in wing cell layers. Ferritin particles were also scattered over the corneal epithelium in all cases. For comparison, normal human corneas and conjunctivas were studied. Ferritin particles were scattered over the corneal epithelium and throughout the basal cells of the conjunctiva. They were not found in corneal stroma or endothelium. In conjunctival stroma, numerous ferritin particles were observed in the cytoplasm of some macrophages. Possible origin of these particles and the cause of their deposition are discussed.", "PMID": 962667}
{"id": "pubmed23n0032_5182", "title": "Intraocular penetration of the soluble antiviral, Ara AMP.", "content": "Earlier studies on intraocular penetration of the antiviral drugs idoxuridine and vidarabine (formerly Ara A) reveal that either inactive metabolites from the former or very low levels of less active metabolites from the latter actually enter the aqueous. Additionally, to achieve therapeutic levels, systemically administered vidarabine must be given in large fluid volumes because of poor solubility. The present time-curve study on the intraocular penetration of the highly soluble nucleotide form of vidarabine, Ara AMP, reveals that, in rabbits, very high aqueous levels of the antiviral metabolite, Ara Hx, are achieved after intravenous does in small fluid volume. Moderately low levels are attained after subconjunctival injection, extremely low levels are attained after subconjunctival injection, and extremely low levels are present after topical administration. This indicates that intravenous  and possibly subconjunctival injection may be useful in treatment of deep ocular herpetic disease.", "contents": "Intraocular penetration of the soluble antiviral, Ara AMP. Earlier studies on intraocular penetration of the antiviral drugs idoxuridine and vidarabine (formerly Ara A) reveal that either inactive metabolites from the former or very low levels of less active metabolites from the latter actually enter the aqueous. Additionally, to achieve therapeutic levels, systemically administered vidarabine must be given in large fluid volumes because of poor solubility. The present time-curve study on the intraocular penetration of the highly soluble nucleotide form of vidarabine, Ara AMP, reveals that, in rabbits, very high aqueous levels of the antiviral metabolite, Ara Hx, are achieved after intravenous does in small fluid volume. Moderately low levels are attained after subconjunctival injection, extremely low levels are attained after subconjunctival injection, and extremely low levels are present after topical administration. This indicates that intravenous  and possibly subconjunctival injection may be useful in treatment of deep ocular herpetic disease.", "PMID": 962668}
{"id": "pubmed23n0032_5183", "title": "Visolett magnifier. Evaluation and optics.", "content": "The Visolett is a glass, nonspectacle magnifier that rests on the paper, producing 1.8X to 2.5X magnification. It is one of the most useful nonspectacle magnifiers, because it is simple to use and diffusely concentrates illumination on its own field, enhancing the magnification. The optics differ from usual hand magnifiers in that the magnification does not increase with the dioptric power.", "contents": "Visolett magnifier. Evaluation and optics. The Visolett is a glass, nonspectacle magnifier that rests on the paper, producing 1.8X to 2.5X magnification. It is one of the most useful nonspectacle magnifiers, because it is simple to use and diffusely concentrates illumination on its own field, enhancing the magnification. The optics differ from usual hand magnifiers in that the magnification does not increase with the dioptric power.", "PMID": 962669}
{"id": "pubmed23n0032_5184", "title": "Fundus photography without a fundus camera.", "content": "A camera for fundus photography can be assembled from a standard indirect ophthalmoscope, a single-lens reflex camera, and a modest amount of easily available additional equipment. This camera can be used to record moderate detail of the posterior portion of the fundus and optic nerve head and can be used in an office, a hospital room, or an operating room.", "contents": "Fundus photography without a fundus camera. A camera for fundus photography can be assembled from a standard indirect ophthalmoscope, a single-lens reflex camera, and a modest amount of easily available additional equipment. This camera can be used to record moderate detail of the posterior portion of the fundus and optic nerve head and can be used in an office, a hospital room, or an operating room.", "PMID": 962670}
{"id": "pubmed23n0032_5185", "title": "Biomicroscopical choroidoscopy (uveoscopy) and transillumination gonioscopy.", "content": "A fiber optic sleeve that fits over a Goldmann three-mirror lens, as well as an independent cable used in conjunction with the Goldmann lens, are described for transillumination biomicroscopical examination of the anterior chamber angle and the uvea. High-intensity retroillumination is provided to the anterior and posterior segments of the globe without production of heat to the eye. Present clinical uses as well as possible future research with the instruments are discussed.", "contents": "Biomicroscopical choroidoscopy (uveoscopy) and transillumination gonioscopy. A fiber optic sleeve that fits over a Goldmann three-mirror lens, as well as an independent cable used in conjunction with the Goldmann lens, are described for transillumination biomicroscopical examination of the anterior chamber angle and the uvea. High-intensity retroillumination is provided to the anterior and posterior segments of the globe without production of heat to the eye. Present clinical uses as well as possible future research with the instruments are discussed.", "PMID": 962671}
{"id": "pubmed23n0032_5186", "title": "[Experimental examinations in plate-prebending--a contribution to the biomechanics of compression osteosynthesis (author's transl)].", "content": "The rigid fixation of bone fragments by compression osteosynthesis with plate produces a high degree of resistance against bending forces, and over the surface friction against rotatory movements. In this way, a uniform distribution of pressure extending over the whole bone section is effected. But by virtue of the asymmetric positioning of the plate on bone-shaft, a torque effect is caused when strain is applied bringing about a distraction of the cortex on the side opposite the plate. Human femoral and tibial specimens would be osteomatized and then stabilized with AO plate adapted to conform to the surface of the bones, under a tension of 70 and 140 kp. With narrow plates having a inferior rigidity there is a greater distraction than is the cause with the wide plates. To compensate against the torque effect, the plates would be prebent. Two measuring methods would be investigate by which the correlation between prestraining and prebending could be determined. To achieve a constant distribution of pressure the narrow plates would have to be prebent to a greater degree than the wide ones, and the DC plates more than the standard plates.", "contents": "[Experimental examinations in plate-prebending--a contribution to the biomechanics of compression osteosynthesis (author's transl)]. The rigid fixation of bone fragments by compression osteosynthesis with plate produces a high degree of resistance against bending forces, and over the surface friction against rotatory movements. In this way, a uniform distribution of pressure extending over the whole bone section is effected. But by virtue of the asymmetric positioning of the plate on bone-shaft, a torque effect is caused when strain is applied bringing about a distraction of the cortex on the side opposite the plate. Human femoral and tibial specimens would be osteomatized and then stabilized with AO plate adapted to conform to the surface of the bones, under a tension of 70 and 140 kp. With narrow plates having a inferior rigidity there is a greater distraction than is the cause with the wide plates. To compensate against the torque effect, the plates would be prebent. Two measuring methods would be investigate by which the correlation between prestraining and prebending could be determined. To achieve a constant distribution of pressure the narrow plates would have to be prebent to a greater degree than the wide ones, and the DC plates more than the standard plates.", "PMID": 962681}
{"id": "pubmed23n0032_5187", "title": "[Release of antibiotics from bone cement and penetration to infected cortical bone (author's transl)].", "content": "Addition of antibiotics to bone cement is used more and more in orthopedics and traumatology. Indications are discussed: 1. infection prophylaxis in replacement arthroplasty, 2. therapy and recidive prophylaxis in infected arthroplasty, 3. therapy of chronic osteomyelitis. Our own experiments show release of antibiotics from bone cement Palacos and penetration into cortical bone after experimental osteomyelitis. Carbenicillin and Cephalotin show high level of concentration for 2 weeks, after 3 weeks there is no concentration evident. Lincomycin and Gentamycin in the first days show high tissue levels, after decrease and increase again after second week. Gentamycin was examined during 10 months showing high levels after this time.", "contents": "[Release of antibiotics from bone cement and penetration to infected cortical bone (author's transl)]. Addition of antibiotics to bone cement is used more and more in orthopedics and traumatology. Indications are discussed: 1. infection prophylaxis in replacement arthroplasty, 2. therapy and recidive prophylaxis in infected arthroplasty, 3. therapy of chronic osteomyelitis. Our own experiments show release of antibiotics from bone cement Palacos and penetration into cortical bone after experimental osteomyelitis. Carbenicillin and Cephalotin show high level of concentration for 2 weeks, after 3 weeks there is no concentration evident. Lincomycin and Gentamycin in the first days show high tissue levels, after decrease and increase again after second week. Gentamycin was examined during 10 months showing high levels after this time.", "PMID": 962682}
{"id": "pubmed23n0032_5188", "title": "Indomethacin-induced delayed fracture healing.", "content": "A patient with an ankle joint fracture-dislocation was preoperatively treated with indomethacin. As in previous animal experiments, indomethacin was found to have an inhibitory effect on bone formation. It is suggested that indomethacin should be used with caution if periosteal fracture healing is anticipated.", "contents": "Indomethacin-induced delayed fracture healing. A patient with an ankle joint fracture-dislocation was preoperatively treated with indomethacin. As in previous animal experiments, indomethacin was found to have an inhibitory effect on bone formation. It is suggested that indomethacin should be used with caution if periosteal fracture healing is anticipated.", "PMID": 962683}
{"id": "pubmed23n0032_5189", "title": "[The dependence of the removal torque of a leg screw a screw surface and implantation time (author's transl)].", "content": "The experiment was performed on 17 sheep tibiae in vivo. 4.5 mm ASIF leg screws with a constant insertion torque (68.6 Ncm) but different surfaces (smooth or rough) were removed after 3 s, 7, 28, 56 and 112 days after insertion. The screws with a rough surface which were implanted for 28 days and longer exhibited a significantly greater removal torque. For instance rough screws which were implanted for 112 days displayed a removal torque eight times greater than after 3 s.", "contents": "[The dependence of the removal torque of a leg screw a screw surface and implantation time (author's transl)]. The experiment was performed on 17 sheep tibiae in vivo. 4.5 mm ASIF leg screws with a constant insertion torque (68.6 Ncm) but different surfaces (smooth or rough) were removed after 3 s, 7, 28, 56 and 112 days after insertion. The screws with a rough surface which were implanted for 28 days and longer exhibited a significantly greater removal torque. For instance rough screws which were implanted for 112 days displayed a removal torque eight times greater than after 3 s.", "PMID": 962684}
{"id": "pubmed23n0032_5190", "title": "[Structure and solidity of new bone in a cortical gliding hole by implanted leg screw (author's transl)].", "content": "Leg screws were implanted in 30 tibia diaphysis. 112 days later in the gliding hole there was new lamellar bone round of the screw head, neck of screw and into the both first screw threads. After screw explantation we measured in 8 cases the solidity of the new bone. Under conditions of tension measurement, we use 341 N per millimeter cortical thread surfaces to put out all new bone from the gliding hole. The results of these tension measurements were cylindric fragments. The remodelling into the gliding hole seems to be the results of static stress and dynamic compensation for reaching a defect in living cortical bone.", "contents": "[Structure and solidity of new bone in a cortical gliding hole by implanted leg screw (author's transl)]. Leg screws were implanted in 30 tibia diaphysis. 112 days later in the gliding hole there was new lamellar bone round of the screw head, neck of screw and into the both first screw threads. After screw explantation we measured in 8 cases the solidity of the new bone. Under conditions of tension measurement, we use 341 N per millimeter cortical thread surfaces to put out all new bone from the gliding hole. The results of these tension measurements were cylindric fragments. The remodelling into the gliding hole seems to be the results of static stress and dynamic compensation for reaching a defect in living cortical bone.", "PMID": 962685}
{"id": "pubmed23n0032_5191", "title": "[Hyperextending ruptures of vessels in course of arthroplasties of the hip and knee (author's transl)].", "content": "The authors referred specific complications in the course of arthroplasties of the hip and of the knee: hyper-extending-ruptures of vessels in sequence of indirect lesions. Causes of these so far not described complications are extreme passive articulations of hip respectively knee during operation. Clinical symptoms are enumerated, the pathogenetic mechanisms explicated and directions for treatment given. Important are the knowledge of these complications and the measure for their prophylaxis.", "contents": "[Hyperextending ruptures of vessels in course of arthroplasties of the hip and knee (author's transl)]. The authors referred specific complications in the course of arthroplasties of the hip and of the knee: hyper-extending-ruptures of vessels in sequence of indirect lesions. Causes of these so far not described complications are extreme passive articulations of hip respectively knee during operation. Clinical symptoms are enumerated, the pathogenetic mechanisms explicated and directions for treatment given. Important are the knowledge of these complications and the measure for their prophylaxis.", "PMID": 962686}
{"id": "pubmed23n0032_5192", "title": "[Causes and treatment of delayed callusformation and pseudarthrosis following fractures of the shaft of the humerus (author's transl)].", "content": "In the OHH, during the years 1969 to 1974, 28 patients were operatively treated because of delayed callusformation or pseud-arthrosis, following a fracture of the shaft of the humerus. It was possible to inquire about and to re-examine 20 patients. The operative treatment had, when possible been achieved by osteosynthesis through plates (25 cases). Each of the so treated cases lead to healing of the bone, additional nerve lesion did not in one case occur because of the operation. Objectively seen, there existed in 7 patients a slight, final restriction of movements, for the abduction and outside rotation in the shoulder joint. 6 patients complained of being sensitive to weather changes. The different treatment method of the fracture of the humerus and pseudarthrosis was discussed.", "contents": "[Causes and treatment of delayed callusformation and pseudarthrosis following fractures of the shaft of the humerus (author's transl)]. In the OHH, during the years 1969 to 1974, 28 patients were operatively treated because of delayed callusformation or pseud-arthrosis, following a fracture of the shaft of the humerus. It was possible to inquire about and to re-examine 20 patients. The operative treatment had, when possible been achieved by osteosynthesis through plates (25 cases). Each of the so treated cases lead to healing of the bone, additional nerve lesion did not in one case occur because of the operation. Objectively seen, there existed in 7 patients a slight, final restriction of movements, for the abduction and outside rotation in the shoulder joint. 6 patients complained of being sensitive to weather changes. The different treatment method of the fracture of the humerus and pseudarthrosis was discussed.", "PMID": 962687}
{"id": "pubmed23n0032_5193", "title": "[Chronic recurrent dislocation of the elbow joint (author's transl)].", "content": "This paper discusses 3 recurrent dislocations of the elbow joint. As possible cause for the etiology of the dislocation the author offers the post-traumatic flattening of the semilunar notch of the ulna. In 2 cases, a successful repair using the Kapel procedure was performed.", "contents": "[Chronic recurrent dislocation of the elbow joint (author's transl)]. This paper discusses 3 recurrent dislocations of the elbow joint. As possible cause for the etiology of the dislocation the author offers the post-traumatic flattening of the semilunar notch of the ulna. In 2 cases, a successful repair using the Kapel procedure was performed.", "PMID": 962688}
{"id": "pubmed23n0032_5194", "title": "[Technique for allogeneic transplantation of knee joint (author's transl)].", "content": "Ever since Lexer's first attempts to remedy destroyed knee-joints by transplanting homioplastic material, the cup joint transplantation as developed by Pap and Krompecher has proved to be expedient and suitable thanks to the recent work performed by Wolkow. In order to assure that the process is safe from the mechanical standpoint, i.e. guarantee an accurate fit of the transplant to the recipient joint portion, in particular in the femur condyle area, the author has developed a method whereby the joint cups are cut rut of the donor material in accordance with pre-produced patterns and the oecipient joint portion is shaped to the correct form by means of similar patterns produced on the basis of X-rays.", "contents": "[Technique for allogeneic transplantation of knee joint (author's transl)]. Ever since Lexer's first attempts to remedy destroyed knee-joints by transplanting homioplastic material, the cup joint transplantation as developed by Pap and Krompecher has proved to be expedient and suitable thanks to the recent work performed by Wolkow. In order to assure that the process is safe from the mechanical standpoint, i.e. guarantee an accurate fit of the transplant to the recipient joint portion, in particular in the femur condyle area, the author has developed a method whereby the joint cups are cut rut of the donor material in accordance with pre-produced patterns and the oecipient joint portion is shaped to the correct form by means of similar patterns produced on the basis of X-rays.", "PMID": 962689}
{"id": "pubmed23n0032_5195", "title": "[Clinical experiences with a new total wrist prosthesis (author's transl)].", "content": "For many individuals the preservation of wrist motion is essential for augmentation of fine motor control of the hand and fingers. When significant functional disability exists at the wrist, currently available surgical treatment includes soft tissue arthroplasty, carpectomy, or arthrodesis. A total wrist joint has recently been developed which offers the patient the potential of a pain-free, stable, and mobile wrist. Based upon the principles of total joint replacement as applied to the hip and knee, the prosthesis is secured to the carpus and radius with methylmethacrylate cement following removal of the navicular, lunate, and head of the capitate. The configuration of the prosthesis interface provides for the two planes of motion normally seen at the wrist; 90 degrees of flexion and extension and 50 degrees of radial ulnar deviation are permitted by the design. Candidates for total wrist replacement are patients who exhibit far advanced disease at the wrist and who might be considered for arthrodesis, but in whom the permanent loss of motion would represent a significant handicap. To date, 20 prostheses have been inserted in 17 patients. The longest follow-up is 14 months. All but 2 patients have gained a useful range of motion. All patients have noted dramatic relief of pain and deformity, permitting them to perform tasks which were preoperatively impossible.", "contents": "[Clinical experiences with a new total wrist prosthesis (author's transl)]. For many individuals the preservation of wrist motion is essential for augmentation of fine motor control of the hand and fingers. When significant functional disability exists at the wrist, currently available surgical treatment includes soft tissue arthroplasty, carpectomy, or arthrodesis. A total wrist joint has recently been developed which offers the patient the potential of a pain-free, stable, and mobile wrist. Based upon the principles of total joint replacement as applied to the hip and knee, the prosthesis is secured to the carpus and radius with methylmethacrylate cement following removal of the navicular, lunate, and head of the capitate. The configuration of the prosthesis interface provides for the two planes of motion normally seen at the wrist; 90 degrees of flexion and extension and 50 degrees of radial ulnar deviation are permitted by the design. Candidates for total wrist replacement are patients who exhibit far advanced disease at the wrist and who might be considered for arthrodesis, but in whom the permanent loss of motion would represent a significant handicap. To date, 20 prostheses have been inserted in 17 patients. The longest follow-up is 14 months. All but 2 patients have gained a useful range of motion. All patients have noted dramatic relief of pain and deformity, permitting them to perform tasks which were preoperatively impossible.", "PMID": 962690}
{"id": "pubmed23n0032_5196", "title": "[The importance, indication and operative technique of dorsal synovectomy of the knee joint in rheumatic diseases (author's transl)].", "content": "From a logical basis, it is reasonable to postulate that a synovectomy must be maximally as complete as possible. The indications and operative technique of radical synovectomy of the dorsal part of the knee joint are presented. The statistically significant improvement of the results of knee synovectomy by an additional dorsal approach is referred to.", "contents": "[The importance, indication and operative technique of dorsal synovectomy of the knee joint in rheumatic diseases (author's transl)]. From a logical basis, it is reasonable to postulate that a synovectomy must be maximally as complete as possible. The indications and operative technique of radical synovectomy of the dorsal part of the knee joint are presented. The statistically significant improvement of the results of knee synovectomy by an additional dorsal approach is referred to.", "PMID": 962691}
{"id": "pubmed23n0032_5197", "title": "[Vascular complications following alloplastic hip replacement (author's transl)].", "content": "Two patients with complications of the pelvic vessels have been seen at our institution after total hip replacement. One had a false aneurysmn of the deep femoral artery, the other presented an arterio-venous fistula. Causes, symptomes and diagnosis of vascular complications during hip replacement are reviewed.", "contents": "[Vascular complications following alloplastic hip replacement (author's transl)]. Two patients with complications of the pelvic vessels have been seen at our institution after total hip replacement. One had a false aneurysmn of the deep femoral artery, the other presented an arterio-venous fistula. Causes, symptomes and diagnosis of vascular complications during hip replacement are reviewed.", "PMID": 962692}
{"id": "pubmed23n0032_5198", "title": "[Differentialdiagnostic of the kneearthrography: bursa--ganglion--capsule rupture (author's transl)].", "content": "Based on 600 arthrogramms physiologic recessus is compared with ganglion for differential-diagnostic purposes. The great variance between small and big recessus of the regio genu posterior will be demonstrated. Flexion of the knee joint favour confusion with ganglion. The radiologic and differential diagnostic valuable criteria of the physiologic recessus and ganglion is shown. Finally the characteristics of Baker's cyst as well as posterior capsule rupture are discussed.", "contents": "[Differentialdiagnostic of the kneearthrography: bursa--ganglion--capsule rupture (author's transl)]. Based on 600 arthrogramms physiologic recessus is compared with ganglion for differential-diagnostic purposes. The great variance between small and big recessus of the regio genu posterior will be demonstrated. Flexion of the knee joint favour confusion with ganglion. The radiologic and differential diagnostic valuable criteria of the physiologic recessus and ganglion is shown. Finally the characteristics of Baker's cyst as well as posterior capsule rupture are discussed.", "PMID": 962693}
{"id": "pubmed23n0032_5199", "title": "[Statistical analysis of congenital hip dysplasia is not possible in the first months of life! (author's transl)].", "content": "The question whether congenital hip dysplasia can be defined statistically has been raised again by Kristen, Dorda and Zweym\u00fcller. These authors believe that a definition is possible by statistical methods. Compiling a normal material for statistical evaluation, however, needs a clinical separation of normal and dysplastic joints before. And this--in our opinion--is not possible during the first half year of life. The only possible way is a long-term follow-up. In our own investigation children with higher acetabular indices have been followed up to 5 years and more. Only by this clinical evaluation is it possible to say which joints have developed normally and which have not. This is a clinical diagnosis of hip dysplasia, not a statistical.", "contents": "[Statistical analysis of congenital hip dysplasia is not possible in the first months of life! (author's transl)]. The question whether congenital hip dysplasia can be defined statistically has been raised again by Kristen, Dorda and Zweym\u00fcller. These authors believe that a definition is possible by statistical methods. Compiling a normal material for statistical evaluation, however, needs a clinical separation of normal and dysplastic joints before. And this--in our opinion--is not possible during the first half year of life. The only possible way is a long-term follow-up. In our own investigation children with higher acetabular indices have been followed up to 5 years and more. Only by this clinical evaluation is it possible to say which joints have developed normally and which have not. This is a clinical diagnosis of hip dysplasia, not a statistical.", "PMID": 962694}
{"id": "pubmed23n0032_5200", "title": "[Scoring system for evaluation of severity and progress of chronic posttraumatic osteomyelitis (author's transl)].", "content": "From the anamnestic data of 50 patients suffering from chronic posttraumatic osteomyelitis a severity scoring system was developed, based on the duration of the disease, the time of hospitalization and the number of surgical interventions. The clinical course of 18 patients, undergoing an autovaccination treatment, was registered by another scoring system (\"Munich-Murnau-Scale\") regarding wound morphology, bone radiology and erythrocyte sedimentation rate.", "contents": "[Scoring system for evaluation of severity and progress of chronic posttraumatic osteomyelitis (author's transl)]. From the anamnestic data of 50 patients suffering from chronic posttraumatic osteomyelitis a severity scoring system was developed, based on the duration of the disease, the time of hospitalization and the number of surgical interventions. The clinical course of 18 patients, undergoing an autovaccination treatment, was registered by another scoring system (\"Munich-Murnau-Scale\") regarding wound morphology, bone radiology and erythrocyte sedimentation rate.", "PMID": 962695}
{"id": "pubmed23n0032_5201", "title": "Experimental evaluation of a new implant material in frontal sinus obliteration: a preliminary report.", "content": "Frontal osteoplasty with exogenous material has been uniformly unsuccessful both experimentally and clinically. Our experiment was designed to test the long-term behavior of a new poly (tetrafluoroethylene)-carbon fiber implant material (Proplast) in a canine frontal sinus model. Varying conditions such as removal of the sinus mucoperiosteum, closure of the nasofrontal ducts, exposure of dura mater, and cerebrospinal fluid leaks were studied at intervals up to one year. Proplast was 100% successful in obliteration of the canine frontal sinus. The ultraporous nature allowed rapid vascularization, collagen ingrowth, and new bone formation, and led to stabilization rather than sequestration. Frontal obliteration with Proplast may be clinically superior to osteoplasty with any other presently available exogenous material or with osteoneogenesis alone, and may even obviate the few complications encountered with adipose implants. Only longer range experimental and clinical evaluation will provide the answer.", "contents": "Experimental evaluation of a new implant material in frontal sinus obliteration: a preliminary report. Frontal osteoplasty with exogenous material has been uniformly unsuccessful both experimentally and clinically. Our experiment was designed to test the long-term behavior of a new poly (tetrafluoroethylene)-carbon fiber implant material (Proplast) in a canine frontal sinus model. Varying conditions such as removal of the sinus mucoperiosteum, closure of the nasofrontal ducts, exposure of dura mater, and cerebrospinal fluid leaks were studied at intervals up to one year. Proplast was 100% successful in obliteration of the canine frontal sinus. The ultraporous nature allowed rapid vascularization, collagen ingrowth, and new bone formation, and led to stabilization rather than sequestration. Frontal obliteration with Proplast may be clinically superior to osteoplasty with any other presently available exogenous material or with osteoneogenesis alone, and may even obviate the few complications encountered with adipose implants. Only longer range experimental and clinical evaluation will provide the answer.", "PMID": 962696}
{"id": "pubmed23n0032_5202", "title": "Dermoid cysts of the head and neck in children.", "content": "The sporadic occurrence of several dermoid cysts of the head and neck in children led us to investigate the relative incidence in a series of 594 epidermal cysts in which 33 such lesions were found. We review the classification of dermoid cysts according to their embryogenesis and location to help avert diagnostic or therapeutic pitfalls. This study emphasizes the importance of adequate general anesthesia and excision of these interesting lesions.", "contents": "Dermoid cysts of the head and neck in children. The sporadic occurrence of several dermoid cysts of the head and neck in children led us to investigate the relative incidence in a series of 594 epidermal cysts in which 33 such lesions were found. We review the classification of dermoid cysts according to their embryogenesis and location to help avert diagnostic or therapeutic pitfalls. This study emphasizes the importance of adequate general anesthesia and excision of these interesting lesions.", "PMID": 962697}
{"id": "pubmed23n0032_5203", "title": "When the weeping stopped: an otologist views otorrhea and gentamicin.", "content": "Gentamicin sulfate ophthalmic solution, 0.3%, was used ototopically in the treatment of 300 patients with otorrhea associated with acute and chronic ear conditions and to obtain a preoperative \"dry state\" in 93 cases in which surgical operation was indicated. Bacterial cultures, antibiograms, and audiometric tests were conducted before, during, and after ototopical therapy. Gentamicin-susceptible pathogens were isolated in all cases. A totally \"dry state\", after three weeks of therapy, was considered evidence of clinical success. Administration of gentamicin was by means of a wick or at the rate of 0.3 to 0.4 ml twice daily. Clinical success was observed in 271 cases. There were no signs of ototoxicity and only one reported instance of skin reaction. This simple, inexpensive treatment is particularly adaptable to situations in which specialized bacteriological testing facilities are unavailable and a broad-spectrum antibiotic is indicated.", "contents": "When the weeping stopped: an otologist views otorrhea and gentamicin. Gentamicin sulfate ophthalmic solution, 0.3%, was used ototopically in the treatment of 300 patients with otorrhea associated with acute and chronic ear conditions and to obtain a preoperative \"dry state\" in 93 cases in which surgical operation was indicated. Bacterial cultures, antibiograms, and audiometric tests were conducted before, during, and after ototopical therapy. Gentamicin-susceptible pathogens were isolated in all cases. A totally \"dry state\", after three weeks of therapy, was considered evidence of clinical success. Administration of gentamicin was by means of a wick or at the rate of 0.3 to 0.4 ml twice daily. Clinical success was observed in 271 cases. There were no signs of ototoxicity and only one reported instance of skin reaction. This simple, inexpensive treatment is particularly adaptable to situations in which specialized bacteriological testing facilities are unavailable and a broad-spectrum antibiotic is indicated.", "PMID": 962698}
{"id": "pubmed23n0032_5204", "title": "Investigation of the mesotympanum's reaction to silastic and gelatin film.", "content": "The section of the cat's mesotympanum, denuded of mucosa, to Silastic and gelatin film was studied and compared with the contralateral control ear, which was also denuded of mucosa. The ears were studied by horizontal pathological sections taken one, two, four, and six months post-lympanotomy and insertion of either gelatin film or Silastic. The Silastic, gelatin film and control ears were compared for inflammatory reaction, amount of fibrosis, and the quality and quantity of mucosal regeneration. The inflammatory reaction was increased in the ears with Silastic compared with their corresponding control ears or to the ears with gelatin film. The amount of fibrosis and the quality and quantity of mucosal regeneration was essentially the same in the Silastic, gelatin film, and control ears. This study shows that both substances are well tolerated in the middle ear and that neither substance stimulated or inhibited the regrowth of mucosa or fibrous tissue when compared with the control.", "contents": "Investigation of the mesotympanum's reaction to silastic and gelatin film. The section of the cat's mesotympanum, denuded of mucosa, to Silastic and gelatin film was studied and compared with the contralateral control ear, which was also denuded of mucosa. The ears were studied by horizontal pathological sections taken one, two, four, and six months post-lympanotomy and insertion of either gelatin film or Silastic. The Silastic, gelatin film and control ears were compared for inflammatory reaction, amount of fibrosis, and the quality and quantity of mucosal regeneration. The inflammatory reaction was increased in the ears with Silastic compared with their corresponding control ears or to the ears with gelatin film. The amount of fibrosis and the quality and quantity of mucosal regeneration was essentially the same in the Silastic, gelatin film, and control ears. This study shows that both substances are well tolerated in the middle ear and that neither substance stimulated or inhibited the regrowth of mucosa or fibrous tissue when compared with the control.", "PMID": 962699}
{"id": "pubmed23n0032_5205", "title": "Mycobacterium fortuitum mastoiditis.", "content": "Improved techniques of bacteriologic identification have led to increasing recognition of the clinical significance of the atypical or anomymous mycobacteria. Mycobacterium fortuitum, included in group IV of Runyon's classification because of its characteristic rapid growth, is widespread in nature as a saprophyte. Its facultative pathogenicity has received increasing attention in the literature recently with reports of a number of isolated infections, epidemics, and deaths. We report a case of mastoiditis due to M fortuitum and discuss the pathogenesis and treatment. This is the first known report of the organism causing otolaryngologic disease.", "contents": "Mycobacterium fortuitum mastoiditis. Improved techniques of bacteriologic identification have led to increasing recognition of the clinical significance of the atypical or anomymous mycobacteria. Mycobacterium fortuitum, included in group IV of Runyon's classification because of its characteristic rapid growth, is widespread in nature as a saprophyte. Its facultative pathogenicity has received increasing attention in the literature recently with reports of a number of isolated infections, epidemics, and deaths. We report a case of mastoiditis due to M fortuitum and discuss the pathogenesis and treatment. This is the first known report of the organism causing otolaryngologic disease.", "PMID": 962700}
{"id": "pubmed23n0032_5206", "title": "Radiation-induced salivary gland tumors: report of a case.", "content": "I discuss radiation-induced salivary gland tumors, with special emphasis on those tumors thought to be secondary to childhood head and neck irradiation for benign diseases. I report such a case and review the literature. Statistically, 77.6% of irradiation-induced tumors occur in the parotid gland and 22.4% in the submaxillary and minor salivary glands. A greater proportion of malignant tumors are noted in the submaxillary and minor salivary glands. At present, there is no demonstrable relationship between tumor occurrence and the amount of radiation recieved. Young children are more susceptible to irradiation-induced salivary tumors than older individuals.", "contents": "Radiation-induced salivary gland tumors: report of a case. I discuss radiation-induced salivary gland tumors, with special emphasis on those tumors thought to be secondary to childhood head and neck irradiation for benign diseases. I report such a case and review the literature. Statistically, 77.6% of irradiation-induced tumors occur in the parotid gland and 22.4% in the submaxillary and minor salivary glands. A greater proportion of malignant tumors are noted in the submaxillary and minor salivary glands. At present, there is no demonstrable relationship between tumor occurrence and the amount of radiation recieved. Young children are more susceptible to irradiation-induced salivary tumors than older individuals.", "PMID": 962701}
{"id": "pubmed23n0032_5207", "title": "Porencephalic cyst of the mastoid.", "content": "A 10-year-old boy developed a porencephalic cyst of the mastoid in conjunction with otitic meningitis and cerebrospinal otorrhea. A defect of the tegmen tympani allowed extrusion of the cyst into the antrum and the mesotympanum. The otorrhea was controlled by closure of the defect. Our review of 251 cases in the literature showed no prior incidence of mastoid involvement by a porencephalic cyst.", "contents": "Porencephalic cyst of the mastoid. A 10-year-old boy developed a porencephalic cyst of the mastoid in conjunction with otitic meningitis and cerebrospinal otorrhea. A defect of the tegmen tympani allowed extrusion of the cyst into the antrum and the mesotympanum. The otorrhea was controlled by closure of the defect. Our review of 251 cases in the literature showed no prior incidence of mastoid involvement by a porencephalic cyst.", "PMID": 962702}
{"id": "pubmed23n0032_5208", "title": "Metastatic adenocarcinoma of the temporal bone.", "content": "Metastatic carcinoma involving the temporal bone is an unusual occurrence. A review of the world literature reveals a total of 102 reported cases. Secondary cancers of the temporal bone arise most frequently from mammary, renal, and bronchogenic carcinomas, all of which show a tendency to metastasize to bone. The pathogenesis of spread to the temporal bone is most commonly by the hematogenous route, but extension from intracranial involvement has also been noted. The symptoms of facial nerve paralysis, otalgia, and aural discharge are consistently associated with patients found to have aural cancers. These symptoms are, however, most frequently indicative of mastoid infection; it is therefore, imperative to consider the possibility of a malignant neoplasm in patients with these symptoms.", "contents": "Metastatic adenocarcinoma of the temporal bone. Metastatic carcinoma involving the temporal bone is an unusual occurrence. A review of the world literature reveals a total of 102 reported cases. Secondary cancers of the temporal bone arise most frequently from mammary, renal, and bronchogenic carcinomas, all of which show a tendency to metastasize to bone. The pathogenesis of spread to the temporal bone is most commonly by the hematogenous route, but extension from intracranial involvement has also been noted. The symptoms of facial nerve paralysis, otalgia, and aural discharge are consistently associated with patients found to have aural cancers. These symptoms are, however, most frequently indicative of mastoid infection; it is therefore, imperative to consider the possibility of a malignant neoplasm in patients with these symptoms.", "PMID": 962703}
{"id": "pubmed23n0032_5209", "title": "Perichondritis of the ear as a complication of acupuncture.", "content": "A 54-year-old woman developed pain, tenderness, and swelling at the site where a short acupuncture needle had been placed in the helix of the pinna. Staphylococcus aureus was isolated from this LESION. Which was treated with removal of the needle and intravenously administered nafcillin. The patient recovered completely and had no scarring.", "contents": "Perichondritis of the ear as a complication of acupuncture. A 54-year-old woman developed pain, tenderness, and swelling at the site where a short acupuncture needle had been placed in the helix of the pinna. Staphylococcus aureus was isolated from this LESION. Which was treated with removal of the needle and intravenously administered nafcillin. The patient recovered completely and had no scarring.", "PMID": 962704}
{"id": "pubmed23n0032_5210", "title": "Factors influencing the choice of fixed-level ear in the ABLB test.", "content": "The use of the worse ear as the fixed-intensity one in the ABLB test has been recommended. This recommendation has been criticised on three grounds. The present work studies these arguments, both theoretically and in light of recent experimental and clinical data, in terms of accuracy of results and of various operational and empirical factors. It is concluded that for achieving each loudness balance it is markedly better to follow the recommendation to keep the worse ear at a fixed intensity and to vary the intensities delivered to the better ear.", "contents": "Factors influencing the choice of fixed-level ear in the ABLB test. The use of the worse ear as the fixed-intensity one in the ABLB test has been recommended. This recommendation has been criticised on three grounds. The present work studies these arguments, both theoretically and in light of recent experimental and clinical data, in terms of accuracy of results and of various operational and empirical factors. It is concluded that for achieving each loudness balance it is markedly better to follow the recommendation to keep the worse ear at a fixed intensity and to vary the intensities delivered to the better ear.", "PMID": 962709}
{"id": "pubmed23n0032_5211", "title": "High-sulphur proteins from alpha-keratins. I. Heterogeneity of the proteins from mouse hair.", "content": "The heterogeneity of the reduced and S-carboxymethylated high-sulphur protein fraction from mouse hair has been examined by chromatography and polyacrylamide gel electrophoresis at pH values above and below the isoelectric region. Considerable heterogeneity is observed both in size (molecular weight range 12000-45000) and in charge. Amino acid analysis of a number of column chromatographic fractions shows the high-sulphur proteins to be largely composed of proteins with a carboxymethylcysteine content above 25 residues and a pronounced heterogeneity in arginine content. Their chromatographic behaviour is similar to that observed for the ultra-high-sulphur proteins from wool.", "contents": "High-sulphur proteins from alpha-keratins. I. Heterogeneity of the proteins from mouse hair. The heterogeneity of the reduced and S-carboxymethylated high-sulphur protein fraction from mouse hair has been examined by chromatography and polyacrylamide gel electrophoresis at pH values above and below the isoelectric region. Considerable heterogeneity is observed both in size (molecular weight range 12000-45000) and in charge. Amino acid analysis of a number of column chromatographic fractions shows the high-sulphur proteins to be largely composed of proteins with a carboxymethylcysteine content above 25 residues and a pronounced heterogeneity in arginine content. Their chromatographic behaviour is similar to that observed for the ultra-high-sulphur proteins from wool.", "PMID": 962716}
{"id": "pubmed23n0032_5212", "title": "Masking level difference: another tool for the evaluation of peripheral and cortical defects.", "content": "Masking level difference (MLD) due to binaural unmasking was measured for speech signals (5-word meaningful sentences) masked by broad-band noise. Tests were carried out in a group of patients with unilateral cerebral lesions of vascular origin and apparently normal pure-tone audiograms (CNS patients), in a control group (normally hearing young adults) and in 5 other groups of patients (conductive symmetrical hearing loss, conductive asymmetrical hearing loss, bilateral presbyacusis, unilateral sudden deafness, Meni\u00e8re's disease). Testing pattern implied three or more S/N ratios in the listening conditions, of SmNm, Sdelta tNo and SmNu (noise correlated), and the speech signal intensity was 70 dB SPL re 20 muPa for the control group, whilst for the pathological cases, speech level intensities were established by means of alternate binaural loudness balance and simultaneous balancing median-plane localization procedures to assess subjective suprathreshold sound image localization at the midline. The results obtained in the control group and in the patients are discussed. With specific reference to the CNS patients, a statistically significant tendency was evident for the binaural condition to produce more MLD when the ear leading in time was ipsilateral to the normal hemisphere. The importance of this and of sensitized speech testing methods in CNS disorders is discussed on the basis of the results obtained in the CNS patients.", "contents": "Masking level difference: another tool for the evaluation of peripheral and cortical defects. Masking level difference (MLD) due to binaural unmasking was measured for speech signals (5-word meaningful sentences) masked by broad-band noise. Tests were carried out in a group of patients with unilateral cerebral lesions of vascular origin and apparently normal pure-tone audiograms (CNS patients), in a control group (normally hearing young adults) and in 5 other groups of patients (conductive symmetrical hearing loss, conductive asymmetrical hearing loss, bilateral presbyacusis, unilateral sudden deafness, Meni\u00e8re's disease). Testing pattern implied three or more S/N ratios in the listening conditions, of SmNm, Sdelta tNo and SmNu (noise correlated), and the speech signal intensity was 70 dB SPL re 20 muPa for the control group, whilst for the pathological cases, speech level intensities were established by means of alternate binaural loudness balance and simultaneous balancing median-plane localization procedures to assess subjective suprathreshold sound image localization at the midline. The results obtained in the control group and in the patients are discussed. With specific reference to the CNS patients, a statistically significant tendency was evident for the binaural condition to produce more MLD when the ear leading in time was ipsilateral to the normal hemisphere. The importance of this and of sensitized speech testing methods in CNS disorders is discussed on the basis of the results obtained in the CNS patients.", "PMID": 962710}
{"id": "pubmed23n0032_5213", "title": "Solid-phase assay for luteinizing hormone in mouse plasma.", "content": "A solid-phase tube assay for measuring LH levels in mouse plasma is described. The assay utilizes an antiserum to ovine LH and ovine LH standards and it measures LH levels in 20 mul of plasma with a sensitivity of less than 0.6 ng/ml. Various parameters affecting the sensitivity and specificity of the assay were investigated. Serial dilutions of plasma from pregnant mice, a pituitary homogenate from mice and plasma from hypophysectomized mice, injected subcutaneously with ovine LH, ran parallel with ovine LH standards in plasma from hypophysectomized mice and plasma with low LH levels from intact mice. Ovine TSH showed about 12% cross reaction in the assay system, whilst rat FSH and prolactin and also ovine FSH, prolactin and GH showed practically no cross reaction. Measurements of plasma LH levels have been made in hypophysectomzied mice after injection with different vehicles containing 10 or 50 mug LH or 50 mug FSH per animal. Daily measurements of LH levels throughout pregnancy in the mouse show a rise in LH level prior to implantationand a further rise around mid-pregnancy which drops off sharply to levels which remain fairly constant until parturition when there is another rise.", "contents": "Solid-phase assay for luteinizing hormone in mouse plasma. A solid-phase tube assay for measuring LH levels in mouse plasma is described. The assay utilizes an antiserum to ovine LH and ovine LH standards and it measures LH levels in 20 mul of plasma with a sensitivity of less than 0.6 ng/ml. Various parameters affecting the sensitivity and specificity of the assay were investigated. Serial dilutions of plasma from pregnant mice, a pituitary homogenate from mice and plasma from hypophysectomized mice, injected subcutaneously with ovine LH, ran parallel with ovine LH standards in plasma from hypophysectomized mice and plasma with low LH levels from intact mice. Ovine TSH showed about 12% cross reaction in the assay system, whilst rat FSH and prolactin and also ovine FSH, prolactin and GH showed practically no cross reaction. Measurements of plasma LH levels have been made in hypophysectomzied mice after injection with different vehicles containing 10 or 50 mug LH or 50 mug FSH per animal. Daily measurements of LH levels throughout pregnancy in the mouse show a rise in LH level prior to implantationand a further rise around mid-pregnancy which drops off sharply to levels which remain fairly constant until parturition when there is another rise.", "PMID": 962717}
{"id": "pubmed23n0032_5214", "title": "High-sulphur proteins from alpha-keratins. II. Isolation and partial characterization of purified components from mouse hair.", "content": "The present paper continues the study of the reduced and S-carboxymethylated high-sulphur proteins from mouse hair. Fractions have been obtained in a substantially purified form by fractional precipitation with ammonium sulphate at pH 6, followed by ion exchange chromatography on cellulose phosphate at pH 2.6. Approximately 80% by weight of the high-sulphur proteins fall into the ultra-high-sulphur category (carboxymethylcysteine content greater than 26 residues per 100 residues), and they cover a molecular weight range of 17000-28000. The components show a remarkable diversity in amino acid composition; for example the contents of arginine and glycine each vary by about 3:1. The remainder of the proteins contain 17-20 residues per 100 residues of carboxymethylcysteine, are smaller in size (molecular weight 11500), and also show great diversity in overall amino acid composition. Molecular weights were determined by chromatography on controlled-pore glass and confirmed by gel filtration on Sephadex G-100 and Sepharose 6B. A comparison of the results suggests that the values obtained should be reliable to within 10%.", "contents": "High-sulphur proteins from alpha-keratins. II. Isolation and partial characterization of purified components from mouse hair. The present paper continues the study of the reduced and S-carboxymethylated high-sulphur proteins from mouse hair. Fractions have been obtained in a substantially purified form by fractional precipitation with ammonium sulphate at pH 6, followed by ion exchange chromatography on cellulose phosphate at pH 2.6. Approximately 80% by weight of the high-sulphur proteins fall into the ultra-high-sulphur category (carboxymethylcysteine content greater than 26 residues per 100 residues), and they cover a molecular weight range of 17000-28000. The components show a remarkable diversity in amino acid composition; for example the contents of arginine and glycine each vary by about 3:1. The remainder of the proteins contain 17-20 residues per 100 residues of carboxymethylcysteine, are smaller in size (molecular weight 11500), and also show great diversity in overall amino acid composition. Molecular weights were determined by chromatography on controlled-pore glass and confirmed by gel filtration on Sephadex G-100 and Sepharose 6B. A comparison of the results suggests that the values obtained should be reliable to within 10%.", "PMID": 962718}
{"id": "pubmed23n0032_5215", "title": "In vitro culture, storage and transfer of goat embryos.", "content": "Goat embryos collected 5 and 7 days after mating, were cultured in vitro at 37 degrees C for 2 days, or stored at 5 degrees C for 1 or 2 days and then cultured for 2 days, or stored in liquid nitrogen (-196 degrees C) for 2-4 weeks and then cultured for 1 day. After culture some of the embryos were transferred to recipient does. Culture and storage was carried out in Dulbecco phosphate buffer enriched with 25% goat serum. 1M glycerol or 2M dimethylsulphoxide (DMSO) was added to the media used for frozen storage. Thirteen of 15 embryos cultured without prior storage showed apparently normal development in culture. Ten of the 13 were transferred and five kids were born. Twenty of 38 embryos stored at 5 degrees C developed in culture and six kids were born following the transfer of 17 embryos. Duration of storage at 5 degrees C had no marked effect upon subsequent development. Six of 48 frozen stored embryos developed in culture. All six were transferred and three kids were born.", "contents": "In vitro culture, storage and transfer of goat embryos. Goat embryos collected 5 and 7 days after mating, were cultured in vitro at 37 degrees C for 2 days, or stored at 5 degrees C for 1 or 2 days and then cultured for 2 days, or stored in liquid nitrogen (-196 degrees C) for 2-4 weeks and then cultured for 1 day. After culture some of the embryos were transferred to recipient does. Culture and storage was carried out in Dulbecco phosphate buffer enriched with 25% goat serum. 1M glycerol or 2M dimethylsulphoxide (DMSO) was added to the media used for frozen storage. Thirteen of 15 embryos cultured without prior storage showed apparently normal development in culture. Ten of the 13 were transferred and five kids were born. Twenty of 38 embryos stored at 5 degrees C developed in culture and six kids were born following the transfer of 17 embryos. Duration of storage at 5 degrees C had no marked effect upon subsequent development. Six of 48 frozen stored embryos developed in culture. All six were transferred and three kids were born.", "PMID": 962719}
{"id": "pubmed23n0032_5216", "title": "The acoustic reflex at low sensation levels.", "content": "The acoustic stapedial reflex, which normally occurs at a hearing level of about 80 dB, can be detected at lower levels by means of preactivation. Two techniques are presented, which allow the detection of reflex activity at intensities from 30 to 50 dB HL. It was the authors' intention to find a way to determine the hearing threshold from the stapedius reflex threshold even in patients, in whom the hearing threshold was not horizontal.", "contents": "The acoustic reflex at low sensation levels. The acoustic stapedial reflex, which normally occurs at a hearing level of about 80 dB, can be detected at lower levels by means of preactivation. Two techniques are presented, which allow the detection of reflex activity at intensities from 30 to 50 dB HL. It was the authors' intention to find a way to determine the hearing threshold from the stapedius reflex threshold even in patients, in whom the hearing threshold was not horizontal.", "PMID": 962715}
{"id": "pubmed23n0032_5217", "title": "Repression, derepression and activation of nitrogenase in Azotobacter vinelandii.", "content": "Ammonium ion repressed nitrogenase in cells fixing N2 gas. Immunological tests and electrophoresis in various gels show that components I (Fe-Mo-S protein) was completely repressed by ammonium, whereas component II (Fe-S protein) apoprotein was not markedly affected. Component II from ammonium-grown cells, however, was inactive since it did not cross react with component I to reduce C2H2 to C2H4. The inactive component II apoprotein is immunologically identical to its active counterpart from cells fixing N2. Identical protein patterns were also observed in various gel-electrophoresis systems. Oxygen-inactivated component II may be reactivated with FeSo4. This salt is preferable to ferrous ammonium sulphate which inactivated component I. Immunodiffusion under aerobic conditions shows that purified component I is composed of aggregated and non-aggregated forms which are antigenically distinct. The aggregate was dissociated by treatment with sodium dodecyl sulphate (SDS) into a single antigenic species which was further resolved into two subunits on SDS disc polyacrylamide gel electrophoresis.", "contents": "Repression, derepression and activation of nitrogenase in Azotobacter vinelandii. Ammonium ion repressed nitrogenase in cells fixing N2 gas. Immunological tests and electrophoresis in various gels show that components I (Fe-Mo-S protein) was completely repressed by ammonium, whereas component II (Fe-S protein) apoprotein was not markedly affected. Component II from ammonium-grown cells, however, was inactive since it did not cross react with component I to reduce C2H2 to C2H4. The inactive component II apoprotein is immunologically identical to its active counterpart from cells fixing N2. Identical protein patterns were also observed in various gel-electrophoresis systems. Oxygen-inactivated component II may be reactivated with FeSo4. This salt is preferable to ferrous ammonium sulphate which inactivated component I. Immunodiffusion under aerobic conditions shows that purified component I is composed of aggregated and non-aggregated forms which are antigenically distinct. The aggregate was dissociated by treatment with sodium dodecyl sulphate (SDS) into a single antigenic species which was further resolved into two subunits on SDS disc polyacrylamide gel electrophoresis.", "PMID": 962720}
{"id": "pubmed23n0032_5218", "title": "Tracking of complex binaural images.", "content": "Subjects were presented with an initially stationary binaural image formed by the fusion of two identical pulses, without interaural time difference (ITD), at the two ears. The image was then made to traverse the subject's auditory perceptual space by introducing ITD, varying linearly with time, under computer control. The direction of movement, i.e. towards the right or left ear, could be reversed by the listener, by pressing a button. Subjects were requested to keep the image central, by pressing the button when they judged that deviation from subjective centre had occurred. Experiments of this type can be considered as analogous to B\u00e9k\u00e9sy audiometry, where the subject automatically traces his threshold of hearing, in that here the listener traces out his auditory perceptual centre as it varies with time. Hence, equivalent analyses to those employed for B\u00e9k\u00e9sy audiometry are possible. Subsequent to the initial part of the experiment, an additional pulse was added to one channel, preceding the original pulse, to form a pulse pair. The monaural masking of the original pulse by this additional pulse thus acts to shift the pre-existing binaural image. The effect of varying the amplitude and onset time of the masking pulse, relative to the original pulse, on the B\u00e9k\u00e9sy-type trace was examined.", "contents": "Tracking of complex binaural images. Subjects were presented with an initially stationary binaural image formed by the fusion of two identical pulses, without interaural time difference (ITD), at the two ears. The image was then made to traverse the subject's auditory perceptual space by introducing ITD, varying linearly with time, under computer control. The direction of movement, i.e. towards the right or left ear, could be reversed by the listener, by pressing a button. Subjects were requested to keep the image central, by pressing the button when they judged that deviation from subjective centre had occurred. Experiments of this type can be considered as analogous to B\u00e9k\u00e9sy audiometry, where the subject automatically traces his threshold of hearing, in that here the listener traces out his auditory perceptual centre as it varies with time. Hence, equivalent analyses to those employed for B\u00e9k\u00e9sy audiometry are possible. Subsequent to the initial part of the experiment, an additional pulse was added to one channel, preceding the original pulse, to form a pulse pair. The monaural masking of the original pulse by this additional pulse thus acts to shift the pre-existing binaural image. The effect of varying the amplitude and onset time of the masking pulse, relative to the original pulse, on the B\u00e9k\u00e9sy-type trace was examined.", "PMID": 962711}
{"id": "pubmed23n0032_5219", "title": "[Early potentials evoked by acoustic stimuli: further results (author's transl)].", "content": "The research work carried out during the last years explains only partially the true nature of the short-latency (less than 50 ms) auditory evoked responses (PEP) in man. Widespread support is given to the hypothesis suggesting a double origin: myogenic and cortical with varying predominance between the two according to the position of the active electrodes and the stimulation parameters. PEP appears to have different characteristics according to the electrode coupling, i.e. inion-lobe, vertex-parietal, and vertex-mastoid. Experiments carried out on normally hearing subjects, on subjects with normal hearing but with marked vestibular signs, and on subjects with severe hearing loss and normal vestibular findings show that the presence of auditory function is one of the essential conditions for the generation of the PEP. Conversely, no influence on PEP is apparent following simultaneous and bilateral inhibition of the steady action potential of the ampullary nerve. Whichever the theoretical origin of the PEP is, it may be concluded that, since the results obtained during the clinical work are still incomplete and controversial, it is not possible to consider the PEP as a true expression of the vestibular function.", "contents": "[Early potentials evoked by acoustic stimuli: further results (author's transl)]. The research work carried out during the last years explains only partially the true nature of the short-latency (less than 50 ms) auditory evoked responses (PEP) in man. Widespread support is given to the hypothesis suggesting a double origin: myogenic and cortical with varying predominance between the two according to the position of the active electrodes and the stimulation parameters. PEP appears to have different characteristics according to the electrode coupling, i.e. inion-lobe, vertex-parietal, and vertex-mastoid. Experiments carried out on normally hearing subjects, on subjects with normal hearing but with marked vestibular signs, and on subjects with severe hearing loss and normal vestibular findings show that the presence of auditory function is one of the essential conditions for the generation of the PEP. Conversely, no influence on PEP is apparent following simultaneous and bilateral inhibition of the steady action potential of the ampullary nerve. Whichever the theoretical origin of the PEP is, it may be concluded that, since the results obtained during the clinical work are still incomplete and controversial, it is not possible to consider the PEP as a true expression of the vestibular function.", "PMID": 962712}
{"id": "pubmed23n0032_5220", "title": "Production of endogenous acetate by the liver in lactating ewes.", "content": "The production of endogenous acetate by the liver has been investigated in lactating ewes using animals with indwelling arterial, and portal and hepatic venous cannulae. The capacity of the liver to produce acetate from acetyl-CoA in vitro has also been examined using homogenates prepared from liver biopsy samples. Mean arterial, portal and hepatic venous blood acetate concentrations in four ewes at 4 weeks lactation were 0.40, 1.00 and 1.46 mM respectively. The mean exogenous and endogenous acetate production rates were 56 and 54 mmol/h respectively, giving a total of 110 MMOL/h. The mean portal-hepatic venous difference in free fatty acid concentration was 81 muM. Converting this uptake of free fatty acids by the liver (based on palmitate as a standard) to 2-carbon equivalents, the acetate produced accounted for 70% of the fatty acids taken up. The correlation coefficient (r2) between uptake of free fatty acids and production of acetate by the liver was 0.83 (P less than 0.01). Calculation of the net acetate production in vivo gave a mean value for the production of acetate of 0.75 nmol/min. Calculation of the in vitro enzymic capacity of the liver to produce acetate from acetyl-CoA gave a mean of 0.94 mmol/min. These results indicate that enzymic production of acetate from acetyl-CoA, via carnitine acetyltransferase and acetylcarnitine hydrolase (see Costa and Snoswell 1975a), can adequately account for the substantial production of acetate by the liver in lactating ewes.", "contents": "Production of endogenous acetate by the liver in lactating ewes. The production of endogenous acetate by the liver has been investigated in lactating ewes using animals with indwelling arterial, and portal and hepatic venous cannulae. The capacity of the liver to produce acetate from acetyl-CoA in vitro has also been examined using homogenates prepared from liver biopsy samples. Mean arterial, portal and hepatic venous blood acetate concentrations in four ewes at 4 weeks lactation were 0.40, 1.00 and 1.46 mM respectively. The mean exogenous and endogenous acetate production rates were 56 and 54 mmol/h respectively, giving a total of 110 MMOL/h. The mean portal-hepatic venous difference in free fatty acid concentration was 81 muM. Converting this uptake of free fatty acids by the liver (based on palmitate as a standard) to 2-carbon equivalents, the acetate produced accounted for 70% of the fatty acids taken up. The correlation coefficient (r2) between uptake of free fatty acids and production of acetate by the liver was 0.83 (P less than 0.01). Calculation of the net acetate production in vivo gave a mean value for the production of acetate of 0.75 nmol/min. Calculation of the in vitro enzymic capacity of the liver to produce acetate from acetyl-CoA gave a mean of 0.94 mmol/min. These results indicate that enzymic production of acetate from acetyl-CoA, via carnitine acetyltransferase and acetylcarnitine hydrolase (see Costa and Snoswell 1975a), can adequately account for the substantial production of acetate by the liver in lactating ewes.", "PMID": 962721}
{"id": "pubmed23n0032_5221", "title": "Intensity and frequency discrimination monitored by evoked response audiometry: clinical application.", "content": "Delta 1 and delta f repetitive stimuli superimposed on continuous 0.5-, 1- and 4-kHz carrier tones were administered to 20 normally hearing persons, 8 adults with sensorineural hearing loss, 10 adults with conductive hearing loss and 22 children subjected to evoked response audiometry (ERA) for assessment of hearing acuity. The intensity modulation depth ranged from + 1 to 10 dB. In the subjects with normal hearing and in the adult patients, three carrier tone levels were used whenever possible: 20, 40, and 60 dB SL. The frequency modulation depth ranged from -1 to -10% of the carrier tone frequency. The same levels for the carrier tone were used: 20, 40, and 60 dB SL. With a carrier tone level of 20 dB SL, a clear-cut vertex response was noted in 80% of the tests carried out in the normal adults with 5-dB delta i stimuli and with delta f stimuli of -3 to -5% in magnitude; the same figures are valid for the patients with sensorineural and conductive hearing loss. The patients with sensorineural hearing loss showed a tendency to give vertex responses with lower delta i stimuli than subjects with normal hearing. However, this difference was not statistically significant. According to our results, the children tested can be roughly divided into two groups, the first including subjects with responses to delta i stimuli of +5 and +10 dB and delta f stimuli of -5% and -10%; the second group including subjects with no clear-cut responses to these stimuli.", "contents": "Intensity and frequency discrimination monitored by evoked response audiometry: clinical application. Delta 1 and delta f repetitive stimuli superimposed on continuous 0.5-, 1- and 4-kHz carrier tones were administered to 20 normally hearing persons, 8 adults with sensorineural hearing loss, 10 adults with conductive hearing loss and 22 children subjected to evoked response audiometry (ERA) for assessment of hearing acuity. The intensity modulation depth ranged from + 1 to 10 dB. In the subjects with normal hearing and in the adult patients, three carrier tone levels were used whenever possible: 20, 40, and 60 dB SL. The frequency modulation depth ranged from -1 to -10% of the carrier tone frequency. The same levels for the carrier tone were used: 20, 40, and 60 dB SL. With a carrier tone level of 20 dB SL, a clear-cut vertex response was noted in 80% of the tests carried out in the normal adults with 5-dB delta i stimuli and with delta f stimuli of -3 to -5% in magnitude; the same figures are valid for the patients with sensorineural and conductive hearing loss. The patients with sensorineural hearing loss showed a tendency to give vertex responses with lower delta i stimuli than subjects with normal hearing. However, this difference was not statistically significant. According to our results, the children tested can be roughly divided into two groups, the first including subjects with responses to delta i stimuli of +5 and +10 dB and delta f stimuli of -5% and -10%; the second group including subjects with no clear-cut responses to these stimuli.", "PMID": 962713}
{"id": "pubmed23n0032_5222", "title": "Studies on monotreme proteins. VII. Amino acid sequence of myoglobin from the platypus, Ornithoryhynchus anatinus.", "content": "Myoglobin isolated from skeletal muscle of the platypus contains 153 amino acid residues. The complete amino acid sequence has been determined following cleavage with cyanogen bromide and further digestion of the four fragments with trypsin, chymotrypsin, pepsin and thermolysin. Sequences of the purified peptides were determined by the dansyl-Edman procedure. The amino acid sequence showed 25 differences from human myoglobin and 24 from kangaroo myoglobin. Amino acid sequences in myoglobins are more conserved than sequences in the alpha- and beta-globin chains, and platypus myoglobin shows a similar number of variations in sequence to kangaroo myoglobin when compared with myoglobin of other species. The date of divergence of the platypus from other mammals was estimated at 102 +/- 31 million years, based on the number of amino acid differences between species and allowing for mutations during the evolutionary period. This estimate differs widely from the estimate given by similar treatment of the alpha- and beta-chain sequences and a constant rate of mutation of globin chains is not supported.", "contents": "Studies on monotreme proteins. VII. Amino acid sequence of myoglobin from the platypus, Ornithoryhynchus anatinus. Myoglobin isolated from skeletal muscle of the platypus contains 153 amino acid residues. The complete amino acid sequence has been determined following cleavage with cyanogen bromide and further digestion of the four fragments with trypsin, chymotrypsin, pepsin and thermolysin. Sequences of the purified peptides were determined by the dansyl-Edman procedure. The amino acid sequence showed 25 differences from human myoglobin and 24 from kangaroo myoglobin. Amino acid sequences in myoglobins are more conserved than sequences in the alpha- and beta-globin chains, and platypus myoglobin shows a similar number of variations in sequence to kangaroo myoglobin when compared with myoglobin of other species. The date of divergence of the platypus from other mammals was estimated at 102 +/- 31 million years, based on the number of amino acid differences between species and allowing for mutations during the evolutionary period. This estimate differs widely from the estimate given by similar treatment of the alpha- and beta-chain sequences and a constant rate of mutation of globin chains is not supported.", "PMID": 962722}
{"id": "pubmed23n0032_5223", "title": "Influence of tonic reflex activities on the slow auditory evoked potential.", "content": "Possible influences of tonic reflex activities on the slow auditory evoked potential were investigated. For this purpose, the slow auditory evoked potential was picked up in 18 volunteers with normal hearing in relaxed position and in postures corresponding to reflex-induced positions and in 15 patients with cerebral palsy (CP) in tonic reflex patterns. No influence on latency for N120 and P215 due to postures could be observed. Latency within CP patients was age-dependent. The effect of the reflex-inhibiting pattern (RIP) - the pattern inhibiting tonic reflex activity in CP patients as far as possible - on potential amplitude was different in the two groups. The effect of RIP in CP patients was comparable to the relaxed position in volunteers. This difference points toward an influence of tonic reflex activity on the slow auditory evoked potential.", "contents": "Influence of tonic reflex activities on the slow auditory evoked potential. Possible influences of tonic reflex activities on the slow auditory evoked potential were investigated. For this purpose, the slow auditory evoked potential was picked up in 18 volunteers with normal hearing in relaxed position and in postures corresponding to reflex-induced positions and in 15 patients with cerebral palsy (CP) in tonic reflex patterns. No influence on latency for N120 and P215 due to postures could be observed. Latency within CP patients was age-dependent. The effect of the reflex-inhibiting pattern (RIP) - the pattern inhibiting tonic reflex activity in CP patients as far as possible - on potential amplitude was different in the two groups. The effect of RIP in CP patients was comparable to the relaxed position in volunteers. This difference points toward an influence of tonic reflex activity on the slow auditory evoked potential.", "PMID": 962714}
{"id": "pubmed23n0032_5224", "title": "Haemoglobins of the shark, Heterodontus portusjacksoni II. Amino acid sequence of the alpha-chain.", "content": "The amino acid sequence of the alpha-chain of the principal haemoglobin from the shark, H. portusjacksoni has been determined. The chain has 148 residues and is acetylated at the amino terminal. The soluble peptides obtained by tryptic and chymotryptic digestion of the protein or its cyanogen bromide fragments were isolated by gel filtration, paper ionophoresis and paper chromatography. The amino acid sequences were determined by the dansyl-Edman procedure. The insoluble \"core\" peptide from the tryptic digestion contained 34 residues and required cleavage by several prosteases before the sequence was established. Compared with human alpha-chain there are 88 amino acid differences including the additional seven residues which appear on the amino terminal of the shark chain. There is also one deletion and one insertion. The chain contains no tryptophan but has four cysteinyl residues which is the highest number of such residues recorded for a vertebrate globin.  In the alpha1beta1 contact sites there are four changes in the oxyhaemoglobin form and six deoxy form. Nine of the 16, alpha1beta1 contact sites show variation while three of the haem contact sites have changed in comparison to the residues known to be involved in these interactions in horse haemoglobin alpha-chain. Use of the sequence data to estimate a time of divergence of the shark from the main vertebrate line yielded the value of 410 +/- 46 million years. The data, in general, support the palaeontological view that bony fishes arose before the elasmobranchs.", "contents": "Haemoglobins of the shark, Heterodontus portusjacksoni II. Amino acid sequence of the alpha-chain. The amino acid sequence of the alpha-chain of the principal haemoglobin from the shark, H. portusjacksoni has been determined. The chain has 148 residues and is acetylated at the amino terminal. The soluble peptides obtained by tryptic and chymotryptic digestion of the protein or its cyanogen bromide fragments were isolated by gel filtration, paper ionophoresis and paper chromatography. The amino acid sequences were determined by the dansyl-Edman procedure. The insoluble \"core\" peptide from the tryptic digestion contained 34 residues and required cleavage by several prosteases before the sequence was established. Compared with human alpha-chain there are 88 amino acid differences including the additional seven residues which appear on the amino terminal of the shark chain. There is also one deletion and one insertion. The chain contains no tryptophan but has four cysteinyl residues which is the highest number of such residues recorded for a vertebrate globin.  In the alpha1beta1 contact sites there are four changes in the oxyhaemoglobin form and six deoxy form. Nine of the 16, alpha1beta1 contact sites show variation while three of the haem contact sites have changed in comparison to the residues known to be involved in these interactions in horse haemoglobin alpha-chain. Use of the sequence data to estimate a time of divergence of the shark from the main vertebrate line yielded the value of 410 +/- 46 million years. The data, in general, support the palaeontological view that bony fishes arose before the elasmobranchs.", "PMID": 962723}
{"id": "pubmed23n0032_5225", "title": "Insect-control chemicals from plants. III. Toxic lignans from Libocedrus bidwillii.", "content": "Feeding tests showed that the powdered dried leaves and leaf extracts of L. bidwilli are toxic to the larvae of the housefly (Musca domestica), and the codling moth (Laspeyresia pomonella). The powdered material was not toxic to the light-brown apple moth (Epiphyas postvittana). The most active toxin is the lignan beta-peltatin-A methyl ether (II) and at a concentration of 100 ppm in a chemically defined diet it gave 98% mortality of housefly larvae.", "contents": "Insect-control chemicals from plants. III. Toxic lignans from Libocedrus bidwillii. Feeding tests showed that the powdered dried leaves and leaf extracts of L. bidwilli are toxic to the larvae of the housefly (Musca domestica), and the codling moth (Laspeyresia pomonella). The powdered material was not toxic to the light-brown apple moth (Epiphyas postvittana). The most active toxin is the lignan beta-peltatin-A methyl ether (II) and at a concentration of 100 ppm in a chemically defined diet it gave 98% mortality of housefly larvae.", "PMID": 962724}
{"id": "pubmed23n0032_5226", "title": "Pulmonary diseases of cattle in the Kimberley District of Western Australia.", "content": "A survey of pulmonary disease in cattle slaughtered in the Kimberley district of Western Australia, incidental to the monitoring of lungs for contagious bovine pleuropneumonia (CBPP), was initiated in 1969. The results of the first 3 years are reported and indicate the complete absence of CBPP, the occurrence of actinobacillosis as the most common pulmonary lesion, the existence of phycomycosis in a few lungs each year, the frequent presence of subacute and chronic bronchopneumonias and interstitial pneumonias possibly as sequelae of actinobacillosis, tuberculosis and the pneumomycoses, and the relatively low incidence of fibrinous and necrotic pneumonias of other bacterial origin.", "contents": "Pulmonary diseases of cattle in the Kimberley District of Western Australia. A survey of pulmonary disease in cattle slaughtered in the Kimberley district of Western Australia, incidental to the monitoring of lungs for contagious bovine pleuropneumonia (CBPP), was initiated in 1969. The results of the first 3 years are reported and indicate the complete absence of CBPP, the occurrence of actinobacillosis as the most common pulmonary lesion, the existence of phycomycosis in a few lungs each year, the frequent presence of subacute and chronic bronchopneumonias and interstitial pneumonias possibly as sequelae of actinobacillosis, tuberculosis and the pneumomycoses, and the relatively low incidence of fibrinous and necrotic pneumonias of other bacterial origin.", "PMID": 962735}
{"id": "pubmed23n0032_5227", "title": "Vaccination of lambs against infection with Taenia ovis.", "content": "A trial was designed to test the efficacy of vaccination of lambs against field challenge with T. ovis eggs using 3 different levels of pasture contamination. Three concentric paddocks (1.62 hectares each) were used and the inner paddock was heavily contaminated with T. ovis eggs by tethering 2 dogs, experimentally infected with T. ovis, at 5 different points in the paddock over a period of 1 month. Four groups of lambs were used. Sixty lambs, from a property known to be free of T. ovis for 2 years previously, were divided into 2 groups of 30 each; one group was vaccinated with T. ovis culture antigen (vaccinated group=V) while the other was vaccinated with culture medium without antigen (sham-vaccinated group=SV). Thirty mature sheep were obtained from a property with a recent record of T. ovis infection (previous natural exposure to infection group=PNE) and 15 lambs were artificially reared from birth under cestode-egg-free conditions (artificially reared groups= AR). Ten animals from groups V, SV, and PNE, and 5 AR lambs were grazed on each of the 3 concentric paddocks for 6 weeks, and then all animals were slaughtered and their total muscle mass (including heart, diaphragm, tongue and masseters) examined, by slicing, for T. ovis cysticerci. The mean total numbers of T. ovis cysticerci recovered were as follows: Inner paddock, PNE 45.5, AR 47.6, SV 107.8, V 6.5; Middle paddock, PNE 5.2, AR 9.0, SV 8.2, V 0.3; Outer paddock, PNE 2.9, AR 2.8, SV 2.3, V 0.2. There were 4 major conclusions from this study. Firstly, vaccination was more effective than previous natural exposure to infection in preventing establishment of new cysticerci. Secondly, although vaccination effectively reduced the numbers of cysticerci, those that became established were mostly viable at the time of slaughter. In contrast, PNE sheep had greater numbers of cysticerci but the majority were degenerating. Vaccination, therefore, did not seem to stimulate a complete immunological response. Thirdly, the presence of T. hydatigena in lambs did not prevent subsequent infection with T. ovis. Fourthly, T. ovis eggs were dispersed from the inner paddock to the outer paddock.", "contents": "Vaccination of lambs against infection with Taenia ovis. A trial was designed to test the efficacy of vaccination of lambs against field challenge with T. ovis eggs using 3 different levels of pasture contamination. Three concentric paddocks (1.62 hectares each) were used and the inner paddock was heavily contaminated with T. ovis eggs by tethering 2 dogs, experimentally infected with T. ovis, at 5 different points in the paddock over a period of 1 month. Four groups of lambs were used. Sixty lambs, from a property known to be free of T. ovis for 2 years previously, were divided into 2 groups of 30 each; one group was vaccinated with T. ovis culture antigen (vaccinated group=V) while the other was vaccinated with culture medium without antigen (sham-vaccinated group=SV). Thirty mature sheep were obtained from a property with a recent record of T. ovis infection (previous natural exposure to infection group=PNE) and 15 lambs were artificially reared from birth under cestode-egg-free conditions (artificially reared groups= AR). Ten animals from groups V, SV, and PNE, and 5 AR lambs were grazed on each of the 3 concentric paddocks for 6 weeks, and then all animals were slaughtered and their total muscle mass (including heart, diaphragm, tongue and masseters) examined, by slicing, for T. ovis cysticerci. The mean total numbers of T. ovis cysticerci recovered were as follows: Inner paddock, PNE 45.5, AR 47.6, SV 107.8, V 6.5; Middle paddock, PNE 5.2, AR 9.0, SV 8.2, V 0.3; Outer paddock, PNE 2.9, AR 2.8, SV 2.3, V 0.2. There were 4 major conclusions from this study. Firstly, vaccination was more effective than previous natural exposure to infection in preventing establishment of new cysticerci. Secondly, although vaccination effectively reduced the numbers of cysticerci, those that became established were mostly viable at the time of slaughter. In contrast, PNE sheep had greater numbers of cysticerci but the majority were degenerating. Vaccination, therefore, did not seem to stimulate a complete immunological response. Thirdly, the presence of T. hydatigena in lambs did not prevent subsequent infection with T. ovis. Fourthly, T. ovis eggs were dispersed from the inner paddock to the outer paddock.", "PMID": 962736}
{"id": "pubmed23n0032_5228", "title": "Genetic resistance to Marek's disease.", "content": "A programme to control MD by genetic selection was tested by selecting breeding sires and dams whose progeny were found to have above average resistance to MD following inoculation at day old with infectious material. Under conditions of natural exposure, Australorp pullets from parents selected for resistance had a lower incidence of MD lesions than an unselected Australorp group. In experimentally challenged Australorp chickens the incidence of MD was significantly lower in progeny derived from mating resistant Australorp sires with unselected dams than in progeny from an unselected Australorp line. Under conditions of experimental and natural exposure to MD, crossbred chickens derived from resistant White Leghorn sires and Australorp dams had a lower incidence of MD lesions than the chickens derived from susceptible White Leghorn series and unselected Australorp dams.", "contents": "Genetic resistance to Marek's disease. A programme to control MD by genetic selection was tested by selecting breeding sires and dams whose progeny were found to have above average resistance to MD following inoculation at day old with infectious material. Under conditions of natural exposure, Australorp pullets from parents selected for resistance had a lower incidence of MD lesions than an unselected Australorp group. In experimentally challenged Australorp chickens the incidence of MD was significantly lower in progeny derived from mating resistant Australorp sires with unselected dams than in progeny from an unselected Australorp line. Under conditions of experimental and natural exposure to MD, crossbred chickens derived from resistant White Leghorn sires and Australorp dams had a lower incidence of MD lesions than the chickens derived from susceptible White Leghorn series and unselected Australorp dams.", "PMID": 962737}
{"id": "pubmed23n0032_5229", "title": "Gastro-intestinal parasites of feral cats in New South Wales.", "content": "A total of 146 feral cats from several localities in New South Wales were examined for the presence of gastro-intestinal parasites. The cestodes recorded, and their prevalence, were Taenia taeniaeformis (21.9%), Spirometra erinacei (61.0%) and Dipylidium caninum (11.6%). One animal contained 31 T. serialis scolices considered to be the contents of a recently ingested Coenurus serialis. The common nematodes encountered and their prevalence were Toxocara cati (21.9%) and Ancylostoma spp (23.3%). Uncinaria stenocephala were found in 2 cats and a stomach wall tumour containing Cyathospirura dasyuridis was found in 1 cat, An acanthocephalan resembling Oncicola canis was the most prevalent parasite among feral cats in this survey. High burdens of S. erinacei, D. caninum, Ancylostoma spp and Oncicola sp were found. Samples from 4 localities in New South Wales were compared and there were major differences in the prevalence of T. taeniaeformis, D. caninum and T. cati. Ancylostoma spp occurred only in the Sydney (53.2%) and the Warren (4.8%) areas. Oncicola sp was not present in the Sydney area, but occurred in the Young area in 23.5% of the feral cats examined and in the Warren area (33.3%) and was most prevalent in the Menindee area (79.2%).", "contents": "Gastro-intestinal parasites of feral cats in New South Wales. A total of 146 feral cats from several localities in New South Wales were examined for the presence of gastro-intestinal parasites. The cestodes recorded, and their prevalence, were Taenia taeniaeformis (21.9%), Spirometra erinacei (61.0%) and Dipylidium caninum (11.6%). One animal contained 31 T. serialis scolices considered to be the contents of a recently ingested Coenurus serialis. The common nematodes encountered and their prevalence were Toxocara cati (21.9%) and Ancylostoma spp (23.3%). Uncinaria stenocephala were found in 2 cats and a stomach wall tumour containing Cyathospirura dasyuridis was found in 1 cat, An acanthocephalan resembling Oncicola canis was the most prevalent parasite among feral cats in this survey. High burdens of S. erinacei, D. caninum, Ancylostoma spp and Oncicola sp were found. Samples from 4 localities in New South Wales were compared and there were major differences in the prevalence of T. taeniaeformis, D. caninum and T. cati. Ancylostoma spp occurred only in the Sydney (53.2%) and the Warren (4.8%) areas. Oncicola sp was not present in the Sydney area, but occurred in the Young area in 23.5% of the feral cats examined and in the Warren area (33.3%) and was most prevalent in the Menindee area (79.2%).", "PMID": 962738}
{"id": "pubmed23n0032_5230", "title": "The comparative incidence of foot rot in Bos taurus and Bos indicus cattle.", "content": "Data on incidence of foot rot were collected over 2 years in a mixed herd of both Bos taurus and Bos indicus breed types. The level of infection was significantly higher in the Bos taurus breeds than in the Bos indicus breeds and raises the possibility of using resistant breeds where foot rot constitutes a major economic problem.", "contents": "The comparative incidence of foot rot in Bos taurus and Bos indicus cattle. Data on incidence of foot rot were collected over 2 years in a mixed herd of both Bos taurus and Bos indicus breed types. The level of infection was significantly higher in the Bos taurus breeds than in the Bos indicus breeds and raises the possibility of using resistant breeds where foot rot constitutes a major economic problem.", "PMID": 962739}
{"id": "pubmed23n0032_5231", "title": "Dermal ulceration of mullet (Mugil cephalus).", "content": "Phycomycotic granulomas are described in the skin of 7 mullet (Mugil cephalus) from extensive outbreaks of dermal ulceration in fish in south-east Queensland. The fungus involved was probably a member of the Family Saprolegniaceae, but was not cultured from the lesions. A myxosporidian infection was present in some fish. The primary cause of the disease is uncertain.", "contents": "Dermal ulceration of mullet (Mugil cephalus). Phycomycotic granulomas are described in the skin of 7 mullet (Mugil cephalus) from extensive outbreaks of dermal ulceration in fish in south-east Queensland. The fungus involved was probably a member of the Family Saprolegniaceae, but was not cultured from the lesions. A myxosporidian infection was present in some fish. The primary cause of the disease is uncertain.", "PMID": 962740}
{"id": "pubmed23n0032_5232", "title": "Effect of furazolidone in feed on immune response of turkeys vaccinated with live Pasteurella multocida in drinking water.", "content": "In 2 trials, turkey poults were fed diets containing 0.011% furazolidone: in one trial until 2, 3, 4, 5, 6, or 7 days before vaccination with live Pasteurella multocida (CU) in the drinking water; and in a second trial with the furazolidone added to the diet 4 days before the vaccination or 1, 3 or 5 days after. This 0.011% level of medication did not appear to reduce the immune response.", "contents": "Effect of furazolidone in feed on immune response of turkeys vaccinated with live Pasteurella multocida in drinking water. In 2 trials, turkey poults were fed diets containing 0.011% furazolidone: in one trial until 2, 3, 4, 5, 6, or 7 days before vaccination with live Pasteurella multocida (CU) in the drinking water; and in a second trial with the furazolidone added to the diet 4 days before the vaccination or 1, 3 or 5 days after. This 0.011% level of medication did not appear to reduce the immune response.", "PMID": 962760}
{"id": "pubmed23n0032_5233", "title": "Isolation of adenoviruses and reoviruses from avian species other than domestic fowl.", "content": "Adenoviruses and reoviruses were isolated from pigeons and mallard ducks. In addition, adenoviruses were isolated from budgerigars and a bantam and a reovirus was isolated from a turkey. Primary identification of these viruses was by electron-microscope examination. It was further possible to assign the 4 adenoviruses to recognized fowl serotypes, and the reoviruses shared a common antigen with fowl reoviruses. These viruses were isolated from a variety of clinical conditions.", "contents": "Isolation of adenoviruses and reoviruses from avian species other than domestic fowl. Adenoviruses and reoviruses were isolated from pigeons and mallard ducks. In addition, adenoviruses were isolated from budgerigars and a bantam and a reovirus was isolated from a turkey. Primary identification of these viruses was by electron-microscope examination. It was further possible to assign the 4 adenoviruses to recognized fowl serotypes, and the reoviruses shared a common antigen with fowl reoviruses. These viruses were isolated from a variety of clinical conditions.", "PMID": 962759}
{"id": "pubmed23n0032_5234", "title": "Further observations on the use of a bivalent bacterin against Haemophilus gallinarum.", "content": "Chickens vaccinated with two doses of a bivalent Haemophilus gallinarum bacterin were protected against seven strains of the organism. Vaccinated and unvaccinated birds with antibody to Mycoplasma gallisepticum had increased HI titers when challenged with H. gallinarum. Birds positive for antibody to Mycoplasma gallisepticum which were not challenged showed no increase in HI titer.", "contents": "Further observations on the use of a bivalent bacterin against Haemophilus gallinarum. Chickens vaccinated with two doses of a bivalent Haemophilus gallinarum bacterin were protected against seven strains of the organism. Vaccinated and unvaccinated birds with antibody to Mycoplasma gallisepticum had increased HI titers when challenged with H. gallinarum. Birds positive for antibody to Mycoplasma gallisepticum which were not challenged showed no increase in HI titer.", "PMID": 962761}
{"id": "pubmed23n0032_5235", "title": "Congenital anomalies of domestic fowl: seven cases.", "content": "In 13,483 necropsies of domestic fowl, six anomalies were recorded: bifurcation of the metasternum, duplication of the ileum, pancreas divisum (partial), vesica fellea occulta, aplasia of the left cecum, and aplasia of the right kidney (2 cases).", "contents": "Congenital anomalies of domestic fowl: seven cases. In 13,483 necropsies of domestic fowl, six anomalies were recorded: bifurcation of the metasternum, duplication of the ileum, pancreas divisum (partial), vesica fellea occulta, aplasia of the left cecum, and aplasia of the right kidney (2 cases).", "PMID": 962762}
{"id": "pubmed23n0032_5236", "title": "Isolation of Mycobacterium avium serotype 3 from a white-headed tree duck (Dendrocygna viduata).", "content": "Tuberculous lesions were observed at necropsy in the liver of 1 of 10 White-headed Tree ducks (Dendrocygna viduata) imported from Nigeria. Microscopic examination revealed granulomas with acid-fast bacilli; Mycobacterium avium serotype 3 was isolated. Two chickens inoculated intraperitoneally with the isolant had gross and microscopic granulomas in the liver at necropsy 62 days after inoculation. Isolants from chickens were serologically similar to the strain isolated from the duck.", "contents": "Isolation of Mycobacterium avium serotype 3 from a white-headed tree duck (Dendrocygna viduata). Tuberculous lesions were observed at necropsy in the liver of 1 of 10 White-headed Tree ducks (Dendrocygna viduata) imported from Nigeria. Microscopic examination revealed granulomas with acid-fast bacilli; Mycobacterium avium serotype 3 was isolated. Two chickens inoculated intraperitoneally with the isolant had gross and microscopic granulomas in the liver at necropsy 62 days after inoculation. Isolants from chickens were serologically similar to the strain isolated from the duck.", "PMID": 962763}
{"id": "pubmed23n0032_5237", "title": "Air sac and renal mucormycosis in an African gray parrot (Psittacus erithacus).", "content": "A case of mucormycosis in an African gray parrot (Psittacus erithacus) involving air sac and kidney is described. The causal fungus, Absidia corymbifera, had produced sporangia in the air-sac lesion.", "contents": "Air sac and renal mucormycosis in an African gray parrot (Psittacus erithacus). A case of mucormycosis in an African gray parrot (Psittacus erithacus) involving air sac and kidney is described. The causal fungus, Absidia corymbifera, had produced sporangia in the air-sac lesion.", "PMID": 962764}
{"id": "pubmed23n0032_5238", "title": "An outbreak of trematodiasis in domestic geese.", "content": "Losses greater than 3% were incurred in a large flock of domestic geese on summer pasture. Necropsy of the sick birds revealed no significant bacterial or viral isolates. The trematodes Echinostoma revolutum and Notocotylus attenuatus were present in considerable numbers, respectively in the small and large intestines.", "contents": "An outbreak of trematodiasis in domestic geese. Losses greater than 3% were incurred in a large flock of domestic geese on summer pasture. Necropsy of the sick birds revealed no significant bacterial or viral isolates. The trematodes Echinostoma revolutum and Notocotylus attenuatus were present in considerable numbers, respectively in the small and large intestines.", "PMID": 962765}
{"id": "pubmed23n0032_5239", "title": "Enhancement of hemagglutinating activity of Haemophilus gallinarum by trypsin.", "content": "It is shown that hemagglutination (HA) activity of Haemophilus gallinarum is enhanced by trypsin. HA activation was more effective at 37 C than at room temperature.", "contents": "Enhancement of hemagglutinating activity of Haemophilus gallinarum by trypsin. It is shown that hemagglutination (HA) activity of Haemophilus gallinarum is enhanced by trypsin. HA activation was more effective at 37 C than at room temperature.", "PMID": 962767}
{"id": "pubmed23n0032_5240", "title": "Neuropsychological servosystems, consciousness, and the problem of embodiment.", "content": "Recent work in neurophysiology has begun to close the paradigmatic gap between neurophysiology and the psychology of embodied behavior and movement. The present paper surveys these developments, traces the origins of the split between neurophysiology and neuropsychology and suggests that recent work by Pribram may provide a link between the psychology of consciousness, the neurophysiology of movement, and the growing body of work on nonverbal communication.", "contents": "Neuropsychological servosystems, consciousness, and the problem of embodiment. Recent work in neurophysiology has begun to close the paradigmatic gap between neurophysiology and the psychology of embodied behavior and movement. The present paper surveys these developments, traces the origins of the split between neurophysiology and neuropsychology and suggests that recent work by Pribram may provide a link between the psychology of consciousness, the neurophysiology of movement, and the growing body of work on nonverbal communication.", "PMID": 962786}
{"id": "pubmed23n0032_5241", "title": "[Influence of autolysis on the quantitative cytoarchitecture of rat hepatocytes. (An ultrastructural morphometric study) (author's transl)].", "content": "Autolysis is very often a reason for cell damage and is also super-imposed on many other cell damages. Already one hour of autolysis causes serious changes in cell metabolism, which can be demonstrated morphometrically. Experiments were made with 10 male adult Wistar rats. 5 animals were for control purposes and 5 animals had to undergo a 1-hour's autolysis. The morphometric analysis of the liver parenchymal cells was based on the information of Weibel et al. (1968) with the help of a computer program. The consequence of the drop in energy caused by autolysis is an enlargement of the liver cell which is possibly due to a breakdown of the energy-dependent ionic pumps. One of the earliest observable cell changes is a so-called \"Kernwandhyperchromatosis\" and chromatin condensation within the nuclei, whereby the Kernwandhyperchromatosis is seen to be a direct consequence of the increase in lactate and decrease of pH. ATP-sufficiency causes a disturbed function of mitochondrial membranes. The microchondria are swollen, the number of mitochondrial grana is clearly reduced. An enlargement of the mitochondrial outer membrane takes place by folding while the surface of mitochondrial cristae remains unchanged. As a consequence of the altered membrane activities also the peroxisomes swell at reduced numerial density. At unchanged total volume of RER the surface of the granulated membranes of the RER decrease by 50%. This decrease caused by ribosome detachment of the granulated membranes corresponds to the enlargement of the degranulated membrane parts of the nedoplasmic reticulum. The vesiculation is caused by an unspecific damage of cytoplasm. While the density of its volume and the membrane surface remain unaltered, the SER also shows a tendency to small vesiculation caused by an unspecific damage of cytoplasm. The increase in number and volume of the lysosomes and vacuoles of unknown origin speaks for a lysosomal activity. The cell compartment responsible for protein synthesis shows the most impressive morphometric and morphologic changes, which eventually can be explained by a decrease of protein synthesis which is needed to obtain enough energy for a well operating physiological equilibrium.", "contents": "[Influence of autolysis on the quantitative cytoarchitecture of rat hepatocytes. (An ultrastructural morphometric study) (author's transl)]. Autolysis is very often a reason for cell damage and is also super-imposed on many other cell damages. Already one hour of autolysis causes serious changes in cell metabolism, which can be demonstrated morphometrically. Experiments were made with 10 male adult Wistar rats. 5 animals were for control purposes and 5 animals had to undergo a 1-hour's autolysis. The morphometric analysis of the liver parenchymal cells was based on the information of Weibel et al. (1968) with the help of a computer program. The consequence of the drop in energy caused by autolysis is an enlargement of the liver cell which is possibly due to a breakdown of the energy-dependent ionic pumps. One of the earliest observable cell changes is a so-called \"Kernwandhyperchromatosis\" and chromatin condensation within the nuclei, whereby the Kernwandhyperchromatosis is seen to be a direct consequence of the increase in lactate and decrease of pH. ATP-sufficiency causes a disturbed function of mitochondrial membranes. The microchondria are swollen, the number of mitochondrial grana is clearly reduced. An enlargement of the mitochondrial outer membrane takes place by folding while the surface of mitochondrial cristae remains unchanged. As a consequence of the altered membrane activities also the peroxisomes swell at reduced numerial density. At unchanged total volume of RER the surface of the granulated membranes of the RER decrease by 50%. This decrease caused by ribosome detachment of the granulated membranes corresponds to the enlargement of the degranulated membrane parts of the nedoplasmic reticulum. The vesiculation is caused by an unspecific damage of cytoplasm. While the density of its volume and the membrane surface remain unaltered, the SER also shows a tendency to small vesiculation caused by an unspecific damage of cytoplasm. The increase in number and volume of the lysosomes and vacuoles of unknown origin speaks for a lysosomal activity. The cell compartment responsible for protein synthesis shows the most impressive morphometric and morphologic changes, which eventually can be explained by a decrease of protein synthesis which is needed to obtain enough energy for a well operating physiological equilibrium.", "PMID": 962787}
{"id": "pubmed23n0032_5242", "title": "Paneth cell tumor. An additional case of duodenal adenoma with malignant change.", "content": "A papillary adenoma with malignant change consisting of only Paneth cells and their precursors is described. Neoplastic proliferation of Paneth cells is discussed from a point of view of cell biology.", "contents": "Paneth cell tumor. An additional case of duodenal adenoma with malignant change. A papillary adenoma with malignant change consisting of only Paneth cells and their precursors is described. Neoplastic proliferation of Paneth cells is discussed from a point of view of cell biology.", "PMID": 962788}
{"id": "pubmed23n0032_5243", "title": "Variations in rat liver chromatin composition during growth and the heterotic response.", "content": "The manifestation of hybrid vigor with regard to the inheritance of body weight was established as a postweaning phenomenon in rats. Liver weight gain generally corresponded to body weight gain and also reflected hybrid vigor. The protein/DNA ratios of the chromatin from livers of inbred and heterotic hybrid male rats were found to increase with increasing age in growing rats. Hybrid chromatin had higher protein/DNA ratios throughout the age range studies. The difference between hybrids and inbreds were greatest in the early postweaning stages of growth and maturation. The RNA/DNA ratios of chromatin from livers of inbred and hybrid male rats were also found to exhibit trends within certain developmental periods.", "contents": "Variations in rat liver chromatin composition during growth and the heterotic response. The manifestation of hybrid vigor with regard to the inheritance of body weight was established as a postweaning phenomenon in rats. Liver weight gain generally corresponded to body weight gain and also reflected hybrid vigor. The protein/DNA ratios of the chromatin from livers of inbred and heterotic hybrid male rats were found to increase with increasing age in growing rats. Hybrid chromatin had higher protein/DNA ratios throughout the age range studies. The difference between hybrids and inbreds were greatest in the early postweaning stages of growth and maturation. The RNA/DNA ratios of chromatin from livers of inbred and hybrid male rats were also found to exhibit trends within certain developmental periods.", "PMID": 962844}
{"id": "pubmed23n0032_5244", "title": "Gene action in fish of tetraploid origin. IV. Ribosomal DNA amount in clupeoid and salmonoid fish.", "content": "Phylogenetically tetraploid species of the fish order Isospondyli generally have twice the mean ribosomal gene content as closely related species on the phylogenetically diploid level. Considerable intraspecific variation of rDNA amount was observed. These findings are discussed in view of the hypothesis that selective loss of ribosomal genes may account for diminishing genic activity in phylogenetically tetraploid organisms.", "contents": "Gene action in fish of tetraploid origin. IV. Ribosomal DNA amount in clupeoid and salmonoid fish. Phylogenetically tetraploid species of the fish order Isospondyli generally have twice the mean ribosomal gene content as closely related species on the phylogenetically diploid level. Considerable intraspecific variation of rDNA amount was observed. These findings are discussed in view of the hypothesis that selective loss of ribosomal genes may account for diminishing genic activity in phylogenetically tetraploid organisms.", "PMID": 962845}
{"id": "pubmed23n0032_5245", "title": "Allelic polymorphism in arabian camel ribonuclease and the amino acid sequence of bactrian camel ribonuclease.", "content": "Pancreatic ribonucleases from several species (whitetail deer, roe deer, guinea pig, and arabian camel) exhibit more than one amino acid at particular positions in their amino acid sequences. Since these enzymes were isolated from pooled pancreas, the origin of this heterogeneity is not clear. The pancreatic ribonucleases from 11 individual arabian camels (Camelus dromedarius) have been investigated with respect to the lysine-glutamine heterogeneity at position 103 (Welling et al., 1975). Six ribonucleases showed only one basic band and five showed two bands after polyacrylamide gel electrophoresis, suggesting a gene frequency of about 0.75 for the Lys gene and about 0.25 for the Gln gene. The amino acid sequence of bactrian camel (Camelus bactrianus) ribonuclease isolated from individual pancreatic tissue was determined and compared with that of arabian camel ribonuclease. The only difference was observed at position 103. In the ribonucleases from two unrelated bactrian camels, only glutamine was observed at that position.", "contents": "Allelic polymorphism in arabian camel ribonuclease and the amino acid sequence of bactrian camel ribonuclease. Pancreatic ribonucleases from several species (whitetail deer, roe deer, guinea pig, and arabian camel) exhibit more than one amino acid at particular positions in their amino acid sequences. Since these enzymes were isolated from pooled pancreas, the origin of this heterogeneity is not clear. The pancreatic ribonucleases from 11 individual arabian camels (Camelus dromedarius) have been investigated with respect to the lysine-glutamine heterogeneity at position 103 (Welling et al., 1975). Six ribonucleases showed only one basic band and five showed two bands after polyacrylamide gel electrophoresis, suggesting a gene frequency of about 0.75 for the Lys gene and about 0.25 for the Gln gene. The amino acid sequence of bactrian camel (Camelus bactrianus) ribonuclease isolated from individual pancreatic tissue was determined and compared with that of arabian camel ribonuclease. The only difference was observed at position 103. In the ribonucleases from two unrelated bactrian camels, only glutamine was observed at that position.", "PMID": 962846}
{"id": "pubmed23n0032_5246", "title": "Strain distribution and linkage relationship of a mouse embryonic hemoglobin variant.", "content": "Search for structural variants of three globin chains (x, y, z), synthesized only during mouse embryonic hematopoiesis, was carried out by electrophoretic analysis of blood from 12-day embryos, all with C57BL/6 mothers, and fathers from 115 inbred stocks selected for their diverse genetic origins. Structure of the beta-chains of adult hemoglobins differed among the tested strains, with 57 carrying the Hbbs allele, 56 the Hbbd allele, and two the Hbbp allele. The search revealed no x- or z-chain variants but confirmed and extended knowledge of a previously described y-chain variant. Blood of all embryos sired by males from the 57 Hbbs strains contained only y'-chains, while blood of au embryos sired by Hbbd or Hbbp males contained y2-chains as well as the y1-chains inherited from their C57BL/6 mother. The locus controlling structure of the y-chain of mouse embryonic hemoglobins is thus extremely closely linked to the locus controlling structure of adult hemoglobin beta-chain, with maximum possible recombination frequency less than 0.019.", "contents": "Strain distribution and linkage relationship of a mouse embryonic hemoglobin variant. Search for structural variants of three globin chains (x, y, z), synthesized only during mouse embryonic hematopoiesis, was carried out by electrophoretic analysis of blood from 12-day embryos, all with C57BL/6 mothers, and fathers from 115 inbred stocks selected for their diverse genetic origins. Structure of the beta-chains of adult hemoglobins differed among the tested strains, with 57 carrying the Hbbs allele, 56 the Hbbd allele, and two the Hbbp allele. The search revealed no x- or z-chain variants but confirmed and extended knowledge of a previously described y-chain variant. Blood of all embryos sired by males from the 57 Hbbs strains contained only y'-chains, while blood of au embryos sired by Hbbd or Hbbp males contained y2-chains as well as the y1-chains inherited from their C57BL/6 mother. The locus controlling structure of the y-chain of mouse embryonic hemoglobins is thus extremely closely linked to the locus controlling structure of adult hemoglobin beta-chain, with maximum possible recombination frequency less than 0.019.", "PMID": 962848}
{"id": "pubmed23n0032_5247", "title": "Genetic and developmental variation of hemoglobin in the deermouse, Peromyscus maniculatus.", "content": "A genetic investigation of electrophoretic hemoglobin variants of the deermouse, Peromyscus maniculatus, shows three alleles, Hblf, Hblr, and Hblo, at a duplicated site controlling the six adult phenotypes. The Hblf allele has not been described previously. The hemoglobin locus is not closely linked to the albino locus. Fetal hemoglobin is distinct from any of the adult components and has a slower electrophoretic mobility. The fetal phenotype changes to the adult type between the days 15 and 18 of prenatal life.", "contents": "Genetic and developmental variation of hemoglobin in the deermouse, Peromyscus maniculatus. A genetic investigation of electrophoretic hemoglobin variants of the deermouse, Peromyscus maniculatus, shows three alleles, Hblf, Hblr, and Hblo, at a duplicated site controlling the six adult phenotypes. The Hblf allele has not been described previously. The hemoglobin locus is not closely linked to the albino locus. Fetal hemoglobin is distinct from any of the adult components and has a slower electrophoretic mobility. The fetal phenotype changes to the adult type between the days 15 and 18 of prenatal life.", "PMID": 962849}
{"id": "pubmed23n0032_5248", "title": "Isolation, determination of structure and synthesis of the acid-labile conjugate of aldosterone.", "content": "1. After administration of 600mg of 3H-labelled aldosterone to human volunteers, 57 mg of homogeneous acid-labile conjugate was isolated from the urine and identified as aldosterone 18 beta-D-glucosiduronic acid. 2. Esterification and acetylation of the conjugate gave a tetra-acetate methyl ester, which, by measurement of the optical rotation and nuclear-magnetic-resonance spectrum, was shown to be a beta-glucosiduronate. This tetra-acetate methyl ester was synthesized in approx. 10% yield by the Koenigs-Knorr procedure. 3. Removal of the acetyl and methyl ester groups from the tetra-acetate methyl ester with alkali was accompanied by almost complete isomerization at C-17 to give 17-isoaldosterone 18 beta-D-glucosiduronic acid. 4. To prevent inversion at C-17 during removal of the acetate and ester groups of beta-glucosiduronate (a) the 3,20-disemicarbazone was prepared, (b) the acetate and ester groups were removed from the disemicarbazone by treatment with alkali, and (c) the semicarbazone groups were removed from the product at pH 2.0, and aldosterone 18 beta-D-glucosiduronic acid was obtained in 47% overall yield. 5. In the presence of components used to synthesize beta-glucosiduronate by the Koenigs-Knorr reaction this substance is converted slowly into the alpha-glucosiduronate; this conversion is responsible, in part, for the low yield of beta-glucosiduronate. 6. Two additional conjugates were obtained in the Koenigs-Knorr reaction; a provisional structure was assigned to one substrate. The other substance is a C-18 alpha-glucosiduronate. Removal of the acetyl and ester groups from C-18 alpha-glucosiduronate gave the alpha-glucosiduronic acid in 84% yield and the 17-isoaldosterone alpha-glucosiduronic acid in 12% yield. 7. The rate at which several types of beta-glucuronidase hydrolyse the foregoing steroidal alpha- and beta-glucosiduronic acids is given.", "contents": "Isolation, determination of structure and synthesis of the acid-labile conjugate of aldosterone. 1. After administration of 600mg of 3H-labelled aldosterone to human volunteers, 57 mg of homogeneous acid-labile conjugate was isolated from the urine and identified as aldosterone 18 beta-D-glucosiduronic acid. 2. Esterification and acetylation of the conjugate gave a tetra-acetate methyl ester, which, by measurement of the optical rotation and nuclear-magnetic-resonance spectrum, was shown to be a beta-glucosiduronate. This tetra-acetate methyl ester was synthesized in approx. 10% yield by the Koenigs-Knorr procedure. 3. Removal of the acetyl and methyl ester groups from the tetra-acetate methyl ester with alkali was accompanied by almost complete isomerization at C-17 to give 17-isoaldosterone 18 beta-D-glucosiduronic acid. 4. To prevent inversion at C-17 during removal of the acetate and ester groups of beta-glucosiduronate (a) the 3,20-disemicarbazone was prepared, (b) the acetate and ester groups were removed from the disemicarbazone by treatment with alkali, and (c) the semicarbazone groups were removed from the product at pH 2.0, and aldosterone 18 beta-D-glucosiduronic acid was obtained in 47% overall yield. 5. In the presence of components used to synthesize beta-glucosiduronate by the Koenigs-Knorr reaction this substance is converted slowly into the alpha-glucosiduronate; this conversion is responsible, in part, for the low yield of beta-glucosiduronate. 6. Two additional conjugates were obtained in the Koenigs-Knorr reaction; a provisional structure was assigned to one substrate. The other substance is a C-18 alpha-glucosiduronate. Removal of the acetyl and ester groups from C-18 alpha-glucosiduronate gave the alpha-glucosiduronic acid in 84% yield and the 17-isoaldosterone alpha-glucosiduronic acid in 12% yield. 7. The rate at which several types of beta-glucuronidase hydrolyse the foregoing steroidal alpha- and beta-glucosiduronic acids is given.", "PMID": 962850}
{"id": "pubmed23n0032_5249", "title": "The role of tetrahydrofolate dehydrogenase in the hepatic supply of tetrahydrobiopterin in rats.", "content": "The reduction of 7,8-dihydrobiopterin to 5,6,7,8-tetrahydrobiopterin by rat liver tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate-NADP+ oxidoreductase, EC 1.5.1.3) is competitively inhibited by trimethoprim lactate (apparent Ki 0.285 muM). An apparent Michaelis constant of 43 muM for dihydrobiopterin was obtained, which is 430 times higher than the reported Km for dihydrofolate with this enzyme. The reduction of dihydrobiopterin is thus more susceptible to inhibition by trimethoprim lactate than is the reduction of dihydrofolate. However, intraperitoneal administration of trimethoprim had no significant effect on the hepatic supply of tetrahydrobiopterin in rats.", "contents": "The role of tetrahydrofolate dehydrogenase in the hepatic supply of tetrahydrobiopterin in rats. The reduction of 7,8-dihydrobiopterin to 5,6,7,8-tetrahydrobiopterin by rat liver tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate-NADP+ oxidoreductase, EC 1.5.1.3) is competitively inhibited by trimethoprim lactate (apparent Ki 0.285 muM). An apparent Michaelis constant of 43 muM for dihydrobiopterin was obtained, which is 430 times higher than the reported Km for dihydrofolate with this enzyme. The reduction of dihydrobiopterin is thus more susceptible to inhibition by trimethoprim lactate than is the reduction of dihydrofolate. However, intraperitoneal administration of trimethoprim had no significant effect on the hepatic supply of tetrahydrobiopterin in rats.", "PMID": 962851}
{"id": "pubmed23n0032_5250", "title": "Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera).", "content": "The enzyme cholesterol ester hydrolase (EC 3.1.1.13) was detected in the larvae of the khapra beetle, Trogoderma granarium (Everts). The pH and temperature optima for the enzyme were 6.6 degrees and 37 degrees C respectively. The mol.wt. of the enzyme was 76000-80000. The enzyme was equally effective in hydrolysing cholesteryl acetate, stearate and oleate. Cholesterol derivatives, namely the chloride and the methyl ether, inhibited the enzyme activity almost completely. It was also inhibited completely by p-hydroxymercuribenzoate. This inhibition was reversed by the addition of dithiothreitol, reduced glutathione or cysteine. The enzyme activity was associated predominantly with the 104000 g fraction.", "contents": "Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera). The enzyme cholesterol ester hydrolase (EC 3.1.1.13) was detected in the larvae of the khapra beetle, Trogoderma granarium (Everts). The pH and temperature optima for the enzyme were 6.6 degrees and 37 degrees C respectively. The mol.wt. of the enzyme was 76000-80000. The enzyme was equally effective in hydrolysing cholesteryl acetate, stearate and oleate. Cholesterol derivatives, namely the chloride and the methyl ether, inhibited the enzyme activity almost completely. It was also inhibited completely by p-hydroxymercuribenzoate. This inhibition was reversed by the addition of dithiothreitol, reduced glutathione or cysteine. The enzyme activity was associated predominantly with the 104000 g fraction.", "PMID": 962852}
{"id": "pubmed23n0032_5251", "title": "Dipeptidyl peptidase IV, a kidney brush-border serine peptidase.", "content": "Dipeptidyl peptidase IV, an enzyme that releases dipeptides from substrates with N-terminal sequences of the forms X-Pro-Y or X-Ala-Y, was purified 300-fold from pig kidney cortex. The kidney is the main source of the enzyme, where it is one of the major microvillus-membrane proteins. Several other tissues contained demonstrable activity against the usual assay substrate glycylproline 2-naphthylamide. In the small intestine this activity was greatly enriched in the microvillus fraction. In all tissues examined, the activity was extremely sensitive to inhibition by di-isopropyl phosphorofluoridate (Dip-F), but relatively resistant to inhibition by phenylmethylsulphonyl fluoride. It is a serine proteinase which may be covalently labelled with [32P]Dip-F, and is the only enzyme of this class in the microvillus membrane. The apparent subunit mol.wt. estimated by sodium dodecyl-sulphate/polyacrylamide-gel electrophoresis and by titration with [32P]Dip-F was 130 000. Gel-filtration and sedimentation-equilibrium methods gave values in the region of 280 000, which is consistent with a dimeric structure, a conclusion supported by electron micrographs of the purified enzyme. Among other well-characterized serine proteinases, this enzyme is unique in its membrane location and its large subunit size. Investigation of the mode of attack of the peptidase on oligopeptides revealed that it could hydrolyse certain N-blocked peptides, e.g. Z-Gly-Pro-Leu-Gly-Pro. In this respect it is acting as an endopeptidase and as such may merit reclassification and renaming as microvillus-membrane serine peptidase.", "contents": "Dipeptidyl peptidase IV, a kidney brush-border serine peptidase. Dipeptidyl peptidase IV, an enzyme that releases dipeptides from substrates with N-terminal sequences of the forms X-Pro-Y or X-Ala-Y, was purified 300-fold from pig kidney cortex. The kidney is the main source of the enzyme, where it is one of the major microvillus-membrane proteins. Several other tissues contained demonstrable activity against the usual assay substrate glycylproline 2-naphthylamide. In the small intestine this activity was greatly enriched in the microvillus fraction. In all tissues examined, the activity was extremely sensitive to inhibition by di-isopropyl phosphorofluoridate (Dip-F), but relatively resistant to inhibition by phenylmethylsulphonyl fluoride. It is a serine proteinase which may be covalently labelled with [32P]Dip-F, and is the only enzyme of this class in the microvillus membrane. The apparent subunit mol.wt. estimated by sodium dodecyl-sulphate/polyacrylamide-gel electrophoresis and by titration with [32P]Dip-F was 130 000. Gel-filtration and sedimentation-equilibrium methods gave values in the region of 280 000, which is consistent with a dimeric structure, a conclusion supported by electron micrographs of the purified enzyme. Among other well-characterized serine proteinases, this enzyme is unique in its membrane location and its large subunit size. Investigation of the mode of attack of the peptidase on oligopeptides revealed that it could hydrolyse certain N-blocked peptides, e.g. Z-Gly-Pro-Leu-Gly-Pro. In this respect it is acting as an endopeptidase and as such may merit reclassification and renaming as microvillus-membrane serine peptidase.", "PMID": 962853}
{"id": "pubmed23n0032_5252", "title": "The separation and characterization of the methylumbelliferyl beta-galactosidases of human liver.", "content": "1. A previously uncharacterized form of human liver acid beta-galactosidase (EC 3.2.1.23), possibly a dimer of molecular weight 160 000, was resolved by gel filtration. It has the same ability to hydrolyse GM1 ganglioside as the two other acid beta-galactosidase forms. 2. The low-molecular-weight forms of acid beta-galactosidase undergo salt-dependent aggregation. 3. The high-molecular-weight component may consist of the low-molecular-weight forms bound to membrane fragments. It can be converted completely into a mixture of these forms. 4. The neutral beta-galactosidase activity can be resolved into two forms by DEAE-cellulose chromatography. They differ in their response to Cl-ions. 5. A new nomenclature is suggested for the six beta-galactosidases so far found in human liver. 6. The enzymic constituents of the beta-galactosidase bands resolved by electrophoresis were re-examined. The A band contains three components. A two-dimensional electrophoretic procedure for resolving the A band is described. 7. The effect of neuraminidase treatment on the behaviour of beta-galactosidases in various separation systems is examined.", "contents": "The separation and characterization of the methylumbelliferyl beta-galactosidases of human liver. 1. A previously uncharacterized form of human liver acid beta-galactosidase (EC 3.2.1.23), possibly a dimer of molecular weight 160 000, was resolved by gel filtration. It has the same ability to hydrolyse GM1 ganglioside as the two other acid beta-galactosidase forms. 2. The low-molecular-weight forms of acid beta-galactosidase undergo salt-dependent aggregation. 3. The high-molecular-weight component may consist of the low-molecular-weight forms bound to membrane fragments. It can be converted completely into a mixture of these forms. 4. The neutral beta-galactosidase activity can be resolved into two forms by DEAE-cellulose chromatography. They differ in their response to Cl-ions. 5. A new nomenclature is suggested for the six beta-galactosidases so far found in human liver. 6. The enzymic constituents of the beta-galactosidase bands resolved by electrophoresis were re-examined. The A band contains three components. A two-dimensional electrophoretic procedure for resolving the A band is described. 7. The effect of neuraminidase treatment on the behaviour of beta-galactosidases in various separation systems is examined.", "PMID": 962854}
{"id": "pubmed23n0032_5253", "title": "A steady-state kinetic study of the reaction catalysed by the secondary-amine mono-oxygenase of Pseudomonas aminovorans.", "content": "1. Secondary-amine mono-oxygenase (proposed EC group 1.14.99.-) was partially purified from trimethylamine-grown Pseudomonas aminovorans by (NH4)2SO4 fractionation, gel filtration, hydrophobic chromatography on 5-aminopentylamino-Sepharose, and affinity chromatography on Sepharose-bound NADH. 2. Some problems in the affinity-chromatography step are discussed. 3. A steady-state kinetic analysis varying substrate, oxygen and electron-donor concentrations was performed, which, over the concentration range studied, gave a series of families of approximately parallel double-reciprocal plots. From secondary and tertiary plots, Michaelis constants of 0.160 mM, 0.086 mM and 0.121 mM were obtained for dimethylamine, NADPH and oxygen respectively. 4. Product-inhibition studies supported the postulated Hexa Uni Ping Pong (triple-transfer) reaction mechanism.", "contents": "A steady-state kinetic study of the reaction catalysed by the secondary-amine mono-oxygenase of Pseudomonas aminovorans. 1. Secondary-amine mono-oxygenase (proposed EC group 1.14.99.-) was partially purified from trimethylamine-grown Pseudomonas aminovorans by (NH4)2SO4 fractionation, gel filtration, hydrophobic chromatography on 5-aminopentylamino-Sepharose, and affinity chromatography on Sepharose-bound NADH. 2. Some problems in the affinity-chromatography step are discussed. 3. A steady-state kinetic analysis varying substrate, oxygen and electron-donor concentrations was performed, which, over the concentration range studied, gave a series of families of approximately parallel double-reciprocal plots. From secondary and tertiary plots, Michaelis constants of 0.160 mM, 0.086 mM and 0.121 mM were obtained for dimethylamine, NADPH and oxygen respectively. 4. Product-inhibition studies supported the postulated Hexa Uni Ping Pong (triple-transfer) reaction mechanism.", "PMID": 962855}
{"id": "pubmed23n0032_5254", "title": "Spectroscopic studies of the binding of bilirubin by ligandin and aminoazo-dye-binding protein A.", "content": "Ligandin and aminoazo-dye-binding protein A both bind bilirubin at a single site. Quantitative studies of the interactions using difference spectrophotometry show that at pH 7.0, protein A binds the tetrapyrrole with an association constant (K) greater than or equal to 2 X 10(7) litre/mol, whereas binding by ligandin is slightly weaker (K = 7 X 10(6) litre/mol) at this pH. The protein-bilirubin complexes give rise to absorption and fluorescence spectra quite different from those of unbound bilirubin and also to large Cotton effects. It appears that on binding to both proteins, the ligand is forced into a rigid twisted configuration in a hydrophobic environment. Ligandin and protein A resemble serum albumin in their interactions with bilirubin.", "contents": "Spectroscopic studies of the binding of bilirubin by ligandin and aminoazo-dye-binding protein A. Ligandin and aminoazo-dye-binding protein A both bind bilirubin at a single site. Quantitative studies of the interactions using difference spectrophotometry show that at pH 7.0, protein A binds the tetrapyrrole with an association constant (K) greater than or equal to 2 X 10(7) litre/mol, whereas binding by ligandin is slightly weaker (K = 7 X 10(6) litre/mol) at this pH. The protein-bilirubin complexes give rise to absorption and fluorescence spectra quite different from those of unbound bilirubin and also to large Cotton effects. It appears that on binding to both proteins, the ligand is forced into a rigid twisted configuration in a hydrophobic environment. Ligandin and protein A resemble serum albumin in their interactions with bilirubin.", "PMID": 962856}
{"id": "pubmed23n0032_5255", "title": "Creatine kinase. Modification of the working enzyme.", "content": "Protection against inhibition of creatine kinase by iodoacetamide is measured by the decrease in the rate constant for the inhibition reaction; A mixture of purified substrates at equilibrium protects quite strongly when all the components of the mixture are nearly saturating. The protection by substrates 'working' in the forward direction only (from creatine and MgATP) was measured by carrying out the experiment rapidly at low concentrations of the enzyme; by varying the concentration of substrate it was found that the amount of protection when the substrates of the forward reaction are saturating is about 80% (100% protection would imply a value of zero for the rate constant of the inhibition reaction). The effects of Ca2+ and Mg2+ are compared. It is already known that the complex creatine-NO3--MgADP, which is considered to be either a transition-state analogue or an analogue of an intermediate in the reaction pathway, protects fully against iodoacetamide, whereas creatine and MgADP alone, or together without NO3-, do not protect. This suggests that the degree of protection by the working enzyme represents the proportion of enzyme molecules that have a conformation complementary to a creatine-PO3-MgADP intermediate.", "contents": "Creatine kinase. Modification of the working enzyme. Protection against inhibition of creatine kinase by iodoacetamide is measured by the decrease in the rate constant for the inhibition reaction; A mixture of purified substrates at equilibrium protects quite strongly when all the components of the mixture are nearly saturating. The protection by substrates 'working' in the forward direction only (from creatine and MgATP) was measured by carrying out the experiment rapidly at low concentrations of the enzyme; by varying the concentration of substrate it was found that the amount of protection when the substrates of the forward reaction are saturating is about 80% (100% protection would imply a value of zero for the rate constant of the inhibition reaction). The effects of Ca2+ and Mg2+ are compared. It is already known that the complex creatine-NO3--MgADP, which is considered to be either a transition-state analogue or an analogue of an intermediate in the reaction pathway, protects fully against iodoacetamide, whereas creatine and MgADP alone, or together without NO3-, do not protect. This suggests that the degree of protection by the working enzyme represents the proportion of enzyme molecules that have a conformation complementary to a creatine-PO3-MgADP intermediate.", "PMID": 962857}
{"id": "pubmed23n0032_5256", "title": "Presence of type III collagen in guinea-pig dermal scar.", "content": "Guinea-pig dermal scar was shown to contain type III collagen, and, from densitometric analysis of gel electrophoretograms, it was shown to have a higher concentration than the surrounding dermis. This finding is consistent with the 'embryonic' nature of newly formed dermal wound tissue, reflected in increased hydroxylation of collagen lysine and the presence of dihydroxylysinonorleucine (after reduction) as the major cross-link.", "contents": "Presence of type III collagen in guinea-pig dermal scar. Guinea-pig dermal scar was shown to contain type III collagen, and, from densitometric analysis of gel electrophoretograms, it was shown to have a higher concentration than the surrounding dermis. This finding is consistent with the 'embryonic' nature of newly formed dermal wound tissue, reflected in increased hydroxylation of collagen lysine and the presence of dihydroxylysinonorleucine (after reduction) as the major cross-link.", "PMID": 962858}
{"id": "pubmed23n0032_5257", "title": "Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus.", "content": "Intervertebral disc is a highly specialized cartilaginous tissue, containing two genetic types of collagen (I and II). Analysis of peptides from a CNBr digest of collagen showed that the proportions of I and II varied gradually and inversely across pig annulus fibrosus, with exclusively type I at the extreme outer edge and exclusively type II in the nucleus pulposus.", "contents": "Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus. Intervertebral disc is a highly specialized cartilaginous tissue, containing two genetic types of collagen (I and II). Analysis of peptides from a CNBr digest of collagen showed that the proportions of I and II varied gradually and inversely across pig annulus fibrosus, with exclusively type I at the extreme outer edge and exclusively type II in the nucleus pulposus.", "PMID": 962859}
{"id": "pubmed23n0032_5258", "title": "Elongation of (omega-14C)oleic acid and (omega-14C)nervonic acid.", "content": "During feeding experiments with [omega-14C]oleic acid and [omega-14c]nervonic acid to adult rats, 14C-labelled C26, C28 and C30 fatty acids were recovered from the intestinal mucosa, liver, plasma, kidney and stools. The structures of these fatty acids were determined by g.l.c., radio-g.l.c. and mass spectrometry. The Schmidt and Ginger degradation methods indicated that most of the 14C found in these extra-long fatty acids remained in the omega position. These radioactive extra-long fatty acids were found mainly in the polar lipids of rats killed 3 or 15 h after being fed on labelled oleic acid or nervonic acid. Rats killed 63 h later yielded only traces of these extra-long fatty acids. When the rats were given antibiotics or received the same radioactive fatty acids by intravenous injection, the labelled extra-long fatty acids could not be detected in any of the tissues. We conclude that they were probably synthesized by elongation of oleic acid and nervonic acid by intestinal micro-organisms (probably yeasts) and then absorbed by the intestinal mucosa.", "contents": "Elongation of (omega-14C)oleic acid and (omega-14C)nervonic acid. During feeding experiments with [omega-14C]oleic acid and [omega-14c]nervonic acid to adult rats, 14C-labelled C26, C28 and C30 fatty acids were recovered from the intestinal mucosa, liver, plasma, kidney and stools. The structures of these fatty acids were determined by g.l.c., radio-g.l.c. and mass spectrometry. The Schmidt and Ginger degradation methods indicated that most of the 14C found in these extra-long fatty acids remained in the omega position. These radioactive extra-long fatty acids were found mainly in the polar lipids of rats killed 3 or 15 h after being fed on labelled oleic acid or nervonic acid. Rats killed 63 h later yielded only traces of these extra-long fatty acids. When the rats were given antibiotics or received the same radioactive fatty acids by intravenous injection, the labelled extra-long fatty acids could not be detected in any of the tissues. We conclude that they were probably synthesized by elongation of oleic acid and nervonic acid by intestinal micro-organisms (probably yeasts) and then absorbed by the intestinal mucosa.", "PMID": 962860}
{"id": "pubmed23n0032_5259", "title": "Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component.", "content": "Ribosomes from higher-plant mitochondria contain 5S rRNA, in contrast with the mitochondrial ribosomes of animals and fungi, in which such a component has not been detected. In common with the ribosomes of prokaryotes and chloroplasts, higher-plant mitochondrial ribosomes do not appear to contain an RNA equivalent to the 5.8 S rRNA that is found in eukaryoytes hydrogen-bonded to the largest of the cytoplasmic rRNA species.", "contents": "Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component. Ribosomes from higher-plant mitochondria contain 5S rRNA, in contrast with the mitochondrial ribosomes of animals and fungi, in which such a component has not been detected. In common with the ribosomes of prokaryotes and chloroplasts, higher-plant mitochondrial ribosomes do not appear to contain an RNA equivalent to the 5.8 S rRNA that is found in eukaryoytes hydrogen-bonded to the largest of the cytoplasmic rRNA species.", "PMID": 962861}
{"id": "pubmed23n0032_5260", "title": "An explanation of apparent sudden change in the activation energy of membrane enzymes.", "content": "If an enzyme-lipid mixture forms phases of pure lipid and enzyme-lipid solution, and enzyme activity depends on the composition of the enzyme-lipid solution, the temperature-dependence of lipid solubility in the enzyme-lipid solution leads to apparent sudden changes in enzyme activation energy without activity discontinuities at lipid phase transition temperatures.", "contents": "An explanation of apparent sudden change in the activation energy of membrane enzymes. If an enzyme-lipid mixture forms phases of pure lipid and enzyme-lipid solution, and enzyme activity depends on the composition of the enzyme-lipid solution, the temperature-dependence of lipid solubility in the enzyme-lipid solution leads to apparent sudden changes in enzyme activation energy without activity discontinuities at lipid phase transition temperatures.", "PMID": 962862}
{"id": "pubmed23n0032_5261", "title": "Turkey liver xanthine dehydrogenase. Relation between nicotinamide-adenine dinucleotide oxidoreductase activity and the content of functional enzyme.", "content": "The turkey liver xanthine dehydrogenase-catalysed oxidation of NADH by Methylene Blue, by ferricyanide or by O2 is not dependent on the integrity of the active-centre persulphide groups. By contrast, the NADH-dichlorophenol-indophenol oxidoreductase and NADH-trinitrobenzenesulphonate oxidoreductase activities are directly proportional to the content of functional enzyme. These findings help to identify the sites of egress of electrons to oxidizing substrates.", "contents": "Turkey liver xanthine dehydrogenase. Relation between nicotinamide-adenine dinucleotide oxidoreductase activity and the content of functional enzyme. The turkey liver xanthine dehydrogenase-catalysed oxidation of NADH by Methylene Blue, by ferricyanide or by O2 is not dependent on the integrity of the active-centre persulphide groups. By contrast, the NADH-dichlorophenol-indophenol oxidoreductase and NADH-trinitrobenzenesulphonate oxidoreductase activities are directly proportional to the content of functional enzyme. These findings help to identify the sites of egress of electrons to oxidizing substrates.", "PMID": 962863}
{"id": "pubmed23n0032_5262", "title": "Subcellular localization of ornithine decarboxylase in liver of control and growth-hormone-treated rats.", "content": "1. Ornithine-2-oxo acid aminotransferase activity was inhibited by amino-oxyacetate (10(-5) M). This permitted the measurement of ornithine decarboxylase in the presence of mitochondria by using the 14CO2-trapping technique. 2. Subcellular fractionation of rat liver by differential centrifugation, followed by the assay of ornithine decarboxylase in the presence of amino oxyacetate and of marker enzymes for each fraction, demonstrated that ornithine decarboxylase was located in the cytosol. 3. The greatly increased ornithine decarboxylase activity observed after growth-hormone administration was also found to be localized in the cytosol. 4. The Km of ornithine decarboxylase from rat liver for ornithine was 28 muM. Administration of growth hormone 4 h before death did not affect the apparent affinity of ornithine decarboxylase for ornithine.", "contents": "Subcellular localization of ornithine decarboxylase in liver of control and growth-hormone-treated rats. 1. Ornithine-2-oxo acid aminotransferase activity was inhibited by amino-oxyacetate (10(-5) M). This permitted the measurement of ornithine decarboxylase in the presence of mitochondria by using the 14CO2-trapping technique. 2. Subcellular fractionation of rat liver by differential centrifugation, followed by the assay of ornithine decarboxylase in the presence of amino oxyacetate and of marker enzymes for each fraction, demonstrated that ornithine decarboxylase was located in the cytosol. 3. The greatly increased ornithine decarboxylase activity observed after growth-hormone administration was also found to be localized in the cytosol. 4. The Km of ornithine decarboxylase from rat liver for ornithine was 28 muM. Administration of growth hormone 4 h before death did not affect the apparent affinity of ornithine decarboxylase for ornithine.", "PMID": 962864}
{"id": "pubmed23n0032_5263", "title": "Active-site determinations on forms of mammalian brain and eel acetylcholinesterase.", "content": "Three forms of brain acetylcholinesterase were purified from bovine caudate-nucleus tissue and determined by calibrated gel filtration to have mol.wts. of approx. 120 000 (C), 230 000 (B) and 330 000 (A). [3H]Di-isopropyl phosphorofluoridate (isopropyl moiety labelled) was purified from commercial preparations and its concentration estimated by an enzyme-titration procedure. Brain acetylcholinesterase preparations and enzyme from eel electric tissue were allowed to react with [3H]di-isopropyl phosphorofluridate in phosphate buffer until enzyme activity was inhibited by 98%. Excess of [3H]di-isopropyl phosphorofluoridate that had not reacted was separated from the labelled enzyme protein by gel filtration, or by vacuum filtration or by extensive dialysis. The specificity of active-site labelling was confirmed by use of the enzyme reactivator, pyridine 2-aldoxime. The forms of brain acetylcholinesterase were calculted to contain approximately two (C) four (B) and six (A) active sites per molecule respectively. Acetylcholinesterase (mol.wt. 250 000) from electric-eel tissue was estimated to contain two active sites per molecule. Gradient-gel electrophoresis was used to confirm the estimation of molecular weights of brain acetylcholinesterase forms made by gel filtration. Under the conditions of electrophoresis acetylcholinesterase form A was stable, but form B was converted into a species of approx. 120 000 mol. wt. Similarly, form C of the brain enzyme was converted into a 60 000-mol.wt. form during electrophoresis. These results are in general accord with the suggestion that the multiple forms of brain acetylcholinesterase may be related to the aggregation of a single low-molecular-weight species.", "contents": "Active-site determinations on forms of mammalian brain and eel acetylcholinesterase. Three forms of brain acetylcholinesterase were purified from bovine caudate-nucleus tissue and determined by calibrated gel filtration to have mol.wts. of approx. 120 000 (C), 230 000 (B) and 330 000 (A). [3H]Di-isopropyl phosphorofluoridate (isopropyl moiety labelled) was purified from commercial preparations and its concentration estimated by an enzyme-titration procedure. Brain acetylcholinesterase preparations and enzyme from eel electric tissue were allowed to react with [3H]di-isopropyl phosphorofluridate in phosphate buffer until enzyme activity was inhibited by 98%. Excess of [3H]di-isopropyl phosphorofluoridate that had not reacted was separated from the labelled enzyme protein by gel filtration, or by vacuum filtration or by extensive dialysis. The specificity of active-site labelling was confirmed by use of the enzyme reactivator, pyridine 2-aldoxime. The forms of brain acetylcholinesterase were calculted to contain approximately two (C) four (B) and six (A) active sites per molecule respectively. Acetylcholinesterase (mol.wt. 250 000) from electric-eel tissue was estimated to contain two active sites per molecule. Gradient-gel electrophoresis was used to confirm the estimation of molecular weights of brain acetylcholinesterase forms made by gel filtration. Under the conditions of electrophoresis acetylcholinesterase form A was stable, but form B was converted into a species of approx. 120 000 mol. wt. Similarly, form C of the brain enzyme was converted into a 60 000-mol.wt. form during electrophoresis. These results are in general accord with the suggestion that the multiple forms of brain acetylcholinesterase may be related to the aggregation of a single low-molecular-weight species.", "PMID": 962865}
{"id": "pubmed23n0032_5264", "title": "The purification and properties of ferritin from human serum.", "content": "1. Ferritin has been isolated from the serum of four patients with iron overload by using two methods. 2. In method A, the serum was adjusted to pH 4.8 and heated to 70 degrees C. After removal of denatured protein, ferritin was concentrated and further purified by ion-exchange chromatography and gel filtration. In most cases, only a partial purification was achieved. 3. In method B, ferritin was extracted from the serum with a column of immuno-adsorbent [anti-(human ferritin)] and released from the column with 3M-KSCN. Further purification was achieved by anion-exchange chromatography followed by the removal of remaining contaminating serum proteins by means of a second immunoadsorbent. Purifications of up to 31 000-fold were achieved, and the homogeneity of the final preparations was demonstrated by polyacrylamide-gel electrophoresis. 4. Serum ferritin purified by either method has the same elution volume as human spleen ferritin on gel filtration on Sephadex G-200. Serum ferritin has a relatively low iron content and iron/protein ratios of 0.023 and 0.067 (mug of Fe/mug of protein) were found in two pure preparations. On anion-exchange chromatography serum ferritin has a low affinity for the column when compared with various tissue ferritins. Isoelectric focusing has demonstrated the presence of a high proportion of isoferritins of relatively high pI. 5. Possible mechanisms for the release of ferritin into the circulation are briefly discussed.", "contents": "The purification and properties of ferritin from human serum. 1. Ferritin has been isolated from the serum of four patients with iron overload by using two methods. 2. In method A, the serum was adjusted to pH 4.8 and heated to 70 degrees C. After removal of denatured protein, ferritin was concentrated and further purified by ion-exchange chromatography and gel filtration. In most cases, only a partial purification was achieved. 3. In method B, ferritin was extracted from the serum with a column of immuno-adsorbent [anti-(human ferritin)] and released from the column with 3M-KSCN. Further purification was achieved by anion-exchange chromatography followed by the removal of remaining contaminating serum proteins by means of a second immunoadsorbent. Purifications of up to 31 000-fold were achieved, and the homogeneity of the final preparations was demonstrated by polyacrylamide-gel electrophoresis. 4. Serum ferritin purified by either method has the same elution volume as human spleen ferritin on gel filtration on Sephadex G-200. Serum ferritin has a relatively low iron content and iron/protein ratios of 0.023 and 0.067 (mug of Fe/mug of protein) were found in two pure preparations. On anion-exchange chromatography serum ferritin has a low affinity for the column when compared with various tissue ferritins. Isoelectric focusing has demonstrated the presence of a high proportion of isoferritins of relatively high pI. 5. Possible mechanisms for the release of ferritin into the circulation are briefly discussed.", "PMID": 962866}
{"id": "pubmed23n0032_5265", "title": "Contemporaneous isolation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and poly(A) polymerase from rat liver mitochondria.", "content": "1. Poly(A) polymerase and DNA-dependent RNA polymerase from rat liver mitochondria can be completely separated by using two different chromatographic procedures. 2. Poly(A) polymerase can only incorporate ATP into acid-insoluble material and strongly depends on the addition of an endogenous factor (probably containing a mixture of oligoribonucleotides), but it is not stimulated by DNA. 3. RNA polymerase is fully DNA-dependent and rifampicin-sensitive, but was not stimulated by the endogenous factor mentioned above. 4. The chromatographic behaviour of the two enzymes, together with the properties described, suggest that they represent two different protein molecules.", "contents": "Contemporaneous isolation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and poly(A) polymerase from rat liver mitochondria. 1. Poly(A) polymerase and DNA-dependent RNA polymerase from rat liver mitochondria can be completely separated by using two different chromatographic procedures. 2. Poly(A) polymerase can only incorporate ATP into acid-insoluble material and strongly depends on the addition of an endogenous factor (probably containing a mixture of oligoribonucleotides), but it is not stimulated by DNA. 3. RNA polymerase is fully DNA-dependent and rifampicin-sensitive, but was not stimulated by the endogenous factor mentioned above. 4. The chromatographic behaviour of the two enzymes, together with the properties described, suggest that they represent two different protein molecules.", "PMID": 962867}
{"id": "pubmed23n0032_5266", "title": "Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma.", "content": "The dye Cibacron Blue F-3-GA was conjugated to Sepharose to provide an affinity column for serum albumin. Passage of whole human plasma through a column of Cibacron Blue-Sepharose results in the removal of approx. 98% of the albumin. The latter can be quantitatively recovered by desorption with NaSCN. Albumin-depleted plasma can be readily resolved into discrete fractions by a combination of conventional biochemical techniques. In particular, the resolution of plasma proteins with properties similar to those of native human plasma albumin can readily be accomplished by ion-exchange chromatography of the Sepharose-dye-treated plasma on DEAE-cellulose.", "contents": "Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma. The dye Cibacron Blue F-3-GA was conjugated to Sepharose to provide an affinity column for serum albumin. Passage of whole human plasma through a column of Cibacron Blue-Sepharose results in the removal of approx. 98% of the albumin. The latter can be quantitatively recovered by desorption with NaSCN. Albumin-depleted plasma can be readily resolved into discrete fractions by a combination of conventional biochemical techniques. In particular, the resolution of plasma proteins with properties similar to those of native human plasma albumin can readily be accomplished by ion-exchange chromatography of the Sepharose-dye-treated plasma on DEAE-cellulose.", "PMID": 962868}
{"id": "pubmed23n0032_5267", "title": "Studies on sequencing of peptides from the carboxyl terminus by using the thiocyanate method.", "content": "We report on our experience in applying the thiocyanate method developed by Stark (1968) (Biochemistry 7, 1796-1807) to the sequencing of short peptides from the carboxyl end in free solution. Yields fell to very low levels after three cycles of degradation. The method was time-consuming because of the filtration and freeze-drying stages involved. To overcome these problems, peptides were attached to modified polystyrene polymers for sequential degradation in the solid phase, and a maximum of six amino acids was determined. Also, ribonuclease was attached to active-ester glass beads and sequential degradation was carried out to determine six amino acids at the C-terminal end of this protein.", "contents": "Studies on sequencing of peptides from the carboxyl terminus by using the thiocyanate method. We report on our experience in applying the thiocyanate method developed by Stark (1968) (Biochemistry 7, 1796-1807) to the sequencing of short peptides from the carboxyl end in free solution. Yields fell to very low levels after three cycles of degradation. The method was time-consuming because of the filtration and freeze-drying stages involved. To overcome these problems, peptides were attached to modified polystyrene polymers for sequential degradation in the solid phase, and a maximum of six amino acids was determined. Also, ribonuclease was attached to active-ester glass beads and sequential degradation was carried out to determine six amino acids at the C-terminal end of this protein.", "PMID": 962869}
{"id": "pubmed23n0032_5268", "title": "The amino acid sequence of pike-whale (lesser-rorqual) pancreatic ribonuclease.", "content": "Pancreatic RNAase (ribonuclease) from the pike whale (lesser rorqual, Balaenoptera acutorostrata) was isolated by affinity chromatography. The protein was digested with different proteolytic enzymes. Peptides were isolated by gel filtration, preparative high-voltage paper electrophoresis and paper chromatography. The amino acid sequence of peptides was determined by the dansyl-Edman method. Although we do not have an amino acid composition for the whole protein, all peptide bonds were overlapped by one or more peptides. Residues 85-96 are bridged by a peptide of unstaisfactory composition and the sequence here depends, at least in part, on homology for its confirmation. Another region in which a similar situation obtains is residues 39-40. This pancreatic RNAase differs at 24-33% of the positions from all other mammalian pancreatic RNAases sequenced to date, except for pig RNAase, from which it differs by 19%. This indicates that whale RNAase has evolved independently during the larger part of the evolution of the mammals. Lesser-rorqual pancreatic RNAase is partially glycosidated (30%) at asparagine-76 in an Asn-Ser-Thr sequence (residues 76-78). Pig RNAase also has carbohydrate attached to asparagine-76 and is identical with lesser-rorqual RNAase in residues 76-98. Detailed evidence for the sequence has been deposited as Supplementary Publication SUP 50066 (11 pages) at the British Library Lending Division, Boston Spa, Wetherby, W. Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms ginen in Biochem. J. (1976) 135, 5.", "contents": "The amino acid sequence of pike-whale (lesser-rorqual) pancreatic ribonuclease. Pancreatic RNAase (ribonuclease) from the pike whale (lesser rorqual, Balaenoptera acutorostrata) was isolated by affinity chromatography. The protein was digested with different proteolytic enzymes. Peptides were isolated by gel filtration, preparative high-voltage paper electrophoresis and paper chromatography. The amino acid sequence of peptides was determined by the dansyl-Edman method. Although we do not have an amino acid composition for the whole protein, all peptide bonds were overlapped by one or more peptides. Residues 85-96 are bridged by a peptide of unstaisfactory composition and the sequence here depends, at least in part, on homology for its confirmation. Another region in which a similar situation obtains is residues 39-40. This pancreatic RNAase differs at 24-33% of the positions from all other mammalian pancreatic RNAases sequenced to date, except for pig RNAase, from which it differs by 19%. This indicates that whale RNAase has evolved independently during the larger part of the evolution of the mammals. Lesser-rorqual pancreatic RNAase is partially glycosidated (30%) at asparagine-76 in an Asn-Ser-Thr sequence (residues 76-78). Pig RNAase also has carbohydrate attached to asparagine-76 and is identical with lesser-rorqual RNAase in residues 76-98. Detailed evidence for the sequence has been deposited as Supplementary Publication SUP 50066 (11 pages) at the British Library Lending Division, Boston Spa, Wetherby, W. Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms ginen in Biochem. J. (1976) 135, 5.", "PMID": 962870}
{"id": "pubmed23n0032_5269", "title": "Enzymes of 2-oxo acid degradation and biosynthesis in cell-free extracts of mixed rumen micro-organisms.", "content": "The enzymes of 2-oxo acid decarboxylation and 2-oxo acid synthesis (EC 1.2.7.1 and EC 1.2.7.2) were isolated and partially purified from cell-free extracts of rumen micro-organisms. The lyase was active with pyruvate, 3-hydroxypyruvate and 2-oxobutyrate. The synthase was active with acetate, 2-oxoglutarate or succinate. Pyruvate synthase was separated from pyruvate lyase by Sephadex G-200 gel filtration. With Sephadex filtration, approximate mol.wts. of 310000 and 210000 were determined for pyruvate lyase and pyruvate synthase respectively.", "contents": "Enzymes of 2-oxo acid degradation and biosynthesis in cell-free extracts of mixed rumen micro-organisms. The enzymes of 2-oxo acid decarboxylation and 2-oxo acid synthesis (EC 1.2.7.1 and EC 1.2.7.2) were isolated and partially purified from cell-free extracts of rumen micro-organisms. The lyase was active with pyruvate, 3-hydroxypyruvate and 2-oxobutyrate. The synthase was active with acetate, 2-oxoglutarate or succinate. Pyruvate synthase was separated from pyruvate lyase by Sephadex G-200 gel filtration. With Sephadex filtration, approximate mol.wts. of 310000 and 210000 were determined for pyruvate lyase and pyruvate synthase respectively.", "PMID": 962871}
{"id": "pubmed23n0032_5270", "title": "Deviation from Michaelis-Menten kinetics for fumarase.", "content": "A study of the steady-state kinetics of fumarase over an extended concentration range, using novel methods of analysis, reveals an initial-rate equation of at least fourth degree for malate as substrate at pH 7.0, with no kinetically significant dead-end complex formation even up to concentrations of 100 mM. In the absence of demonstrable enzyme-aggregation phenomena, this is interpreted as indicating co-operative effects overlooked previously, although a mixture of isoenzymes, each individually of high degree and giving a complex curve, may be a contributing factor.", "contents": "Deviation from Michaelis-Menten kinetics for fumarase. A study of the steady-state kinetics of fumarase over an extended concentration range, using novel methods of analysis, reveals an initial-rate equation of at least fourth degree for malate as substrate at pH 7.0, with no kinetically significant dead-end complex formation even up to concentrations of 100 mM. In the absence of demonstrable enzyme-aggregation phenomena, this is interpreted as indicating co-operative effects overlooked previously, although a mixture of isoenzymes, each individually of high degree and giving a complex curve, may be a contributing factor.", "PMID": 962872}
{"id": "pubmed23n0032_5271", "title": "Comparative activity of arylsulphatases A and B on two synthetic substrates.", "content": "Rat liver and human skin fibroblasts arylsulphatase A and B activities on both 4-methylumbelliferyl sulphate and 4-nitrocatechol sulphate were compared. The intracellular distribution of activity differed markedly when 4-methylumbelliferyl sulphate was used from that observed with 4-nitrocatechol sulphate. No discrimination between control and metachromatic leucodystrophy or mucopolysaccharidosis (type VI) could be achieved when 4-methylumbelliferyl sulphate was used as substrate. These results contrast sharply with those obtained with 4-nitrocatechol sulphate and cast doubt on the validity of 4-methylumbelliferyl sulphate as substrate for the determination of arylsulphatase A and B activities.", "contents": "Comparative activity of arylsulphatases A and B on two synthetic substrates. Rat liver and human skin fibroblasts arylsulphatase A and B activities on both 4-methylumbelliferyl sulphate and 4-nitrocatechol sulphate were compared. The intracellular distribution of activity differed markedly when 4-methylumbelliferyl sulphate was used from that observed with 4-nitrocatechol sulphate. No discrimination between control and metachromatic leucodystrophy or mucopolysaccharidosis (type VI) could be achieved when 4-methylumbelliferyl sulphate was used as substrate. These results contrast sharply with those obtained with 4-nitrocatechol sulphate and cast doubt on the validity of 4-methylumbelliferyl sulphate as substrate for the determination of arylsulphatase A and B activities.", "PMID": 962873}
{"id": "pubmed23n0032_5272", "title": "Biotin-binding protein from chicken egg yolk. Assay and relationship to egg-white avidin.", "content": "1. Biotin in chicken egg yolk is non-covalently bound to a specific protein that comprises 0.03% of the total yolk protein (0.8 mg/yolk). This biotin-binding protein is not detectable by the normal avidin assay owing to the biotin being tightly bound. Exchange of [14C]biotin for bound biotin at 65 degrees C is the basis of an assay for this protein. 2. Biotin-binding protein from egg yolk is distinguishable from egg-white avidin on Sephadex G-100 gel filtration, although the sizes of the two proteins appear quite similar. 3. Biotin-binding protein is denatured at a lower temperature and freely exchanges biotin at lower temperatures than does avidin. 4. The biotin-binding protein in egg yolk is postulated to be responsible for the deposition of biotin in egg yolk. D-[carboxyl-14C]Biotin injected into laying hens rapidly appears in the egg bound to yolk biotin-binding protein and avidin. Over 60% of the radioactivity is eventually deposited in eggs. The kinetics of biotin deposition in the egg suggests a 25 day half-life for an intracellular biotinyl-coenzyme pool in the laying hen.", "contents": "Biotin-binding protein from chicken egg yolk. Assay and relationship to egg-white avidin. 1. Biotin in chicken egg yolk is non-covalently bound to a specific protein that comprises 0.03% of the total yolk protein (0.8 mg/yolk). This biotin-binding protein is not detectable by the normal avidin assay owing to the biotin being tightly bound. Exchange of [14C]biotin for bound biotin at 65 degrees C is the basis of an assay for this protein. 2. Biotin-binding protein from egg yolk is distinguishable from egg-white avidin on Sephadex G-100 gel filtration, although the sizes of the two proteins appear quite similar. 3. Biotin-binding protein is denatured at a lower temperature and freely exchanges biotin at lower temperatures than does avidin. 4. The biotin-binding protein in egg yolk is postulated to be responsible for the deposition of biotin in egg yolk. D-[carboxyl-14C]Biotin injected into laying hens rapidly appears in the egg bound to yolk biotin-binding protein and avidin. Over 60% of the radioactivity is eventually deposited in eggs. The kinetics of biotin deposition in the egg suggests a 25 day half-life for an intracellular biotinyl-coenzyme pool in the laying hen.", "PMID": 962874}
{"id": "pubmed23n0032_5273", "title": "The interactions of haem with ligandin and aminoazo-dye-binding protein A.", "content": "1. The interactions of ferriprotoporphyrin IX with ligandin and aminoazo-dye-binding protein A result in absorption spectra in the Soret region characteristic of the ligand in its monomeric state. 2. Both proteins are able to bind ferrous as well as ferric haem. 3. Ferriprotoporphyrin IX is bound at a single site on both proteins. At pH7.0, I 0.16M, difference-spectrophotometric measurements gave association constants of 10(7) and 4 X 10(6) LITRE/MOL FOR LIGANDIN AND PROTEin A respectively. Under the same conditions fluorescence-quenching experiments gave an association constant of 2 X 10(7) litre/mol for ligandin. 4. Bilirubin, bromosulphophthalein and oesterone sulphate each compete with haem for binding by the two proteins. 5. Ferriprotoporphyrin IX bound to both ligandin and protein A is able to form co-ordination complexes with CN-, but not, to any measurable extent, with either N3- or F-. From these results it is suggested that binding by the two proteins may not involve the haem iron atom. 6. Both haem-protein complexes give rise to measurable extrinsic Cotton effects in the Soret region. 7. The formation and properties of the ligandin- and protein A-haem complexes are compared with those of haem-albumin, haemoglobin, myoglobin and other haemoproteins.", "contents": "The interactions of haem with ligandin and aminoazo-dye-binding protein A. 1. The interactions of ferriprotoporphyrin IX with ligandin and aminoazo-dye-binding protein A result in absorption spectra in the Soret region characteristic of the ligand in its monomeric state. 2. Both proteins are able to bind ferrous as well as ferric haem. 3. Ferriprotoporphyrin IX is bound at a single site on both proteins. At pH7.0, I 0.16M, difference-spectrophotometric measurements gave association constants of 10(7) and 4 X 10(6) LITRE/MOL FOR LIGANDIN AND PROTEin A respectively. Under the same conditions fluorescence-quenching experiments gave an association constant of 2 X 10(7) litre/mol for ligandin. 4. Bilirubin, bromosulphophthalein and oesterone sulphate each compete with haem for binding by the two proteins. 5. Ferriprotoporphyrin IX bound to both ligandin and protein A is able to form co-ordination complexes with CN-, but not, to any measurable extent, with either N3- or F-. From these results it is suggested that binding by the two proteins may not involve the haem iron atom. 6. Both haem-protein complexes give rise to measurable extrinsic Cotton effects in the Soret region. 7. The formation and properties of the ligandin- and protein A-haem complexes are compared with those of haem-albumin, haemoglobin, myoglobin and other haemoproteins.", "PMID": 962875}
{"id": "pubmed23n0032_5274", "title": "The identification of 2,8-dihydroxyadenine, a new component of urinary stones.", "content": "Stones passed by a child homozygous for a deficiency of the enzyme adenine phosphoribosyltransferase have been identified by u.v., i.r. and mass spectrometry as 2,8-dihydroxyadenine.", "contents": "The identification of 2,8-dihydroxyadenine, a new component of urinary stones. Stones passed by a child homozygous for a deficiency of the enzyme adenine phosphoribosyltransferase have been identified by u.v., i.r. and mass spectrometry as 2,8-dihydroxyadenine.", "PMID": 962876}
{"id": "pubmed23n0032_5275", "title": "Methods for fitting equations with two or more non-linear parameters.", "content": "1. Descriptions are given of two ways for fitting non-linear equations by least-squares criteria to experimental data. One depends on solving a set of non-linear simultaneous equations, and the other on Taylor's theorem. 2. It is shown that better parameter estimates result when an equation with two or more non-linear parameters is fitted to all the sets of data simultaneously than when it is fitted to each set in turn.", "contents": "Methods for fitting equations with two or more non-linear parameters. 1. Descriptions are given of two ways for fitting non-linear equations by least-squares criteria to experimental data. One depends on solving a set of non-linear simultaneous equations, and the other on Taylor's theorem. 2. It is shown that better parameter estimates result when an equation with two or more non-linear parameters is fitted to all the sets of data simultaneously than when it is fitted to each set in turn.", "PMID": 962877}
{"id": "pubmed23n0032_5276", "title": "Two methods for fitting a rectangular hyperbola to data from several animals.", "content": "Two methods are described for fitting the Michaelis-Menten equation to sets of data with a common michaelis constant but different maximum velocities. One of them uses the method of least squares, and the other is based on the direct plot of Eisenthal & Cornish-Bowden [Biochem. J. (1974) 139, 715-720].", "contents": "Two methods for fitting a rectangular hyperbola to data from several animals. Two methods are described for fitting the Michaelis-Menten equation to sets of data with a common michaelis constant but different maximum velocities. One of them uses the method of least squares, and the other is based on the direct plot of Eisenthal & Cornish-Bowden [Biochem. J. (1974) 139, 715-720].", "PMID": 962878}
{"id": "pubmed23n0032_5277", "title": "Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath.", "content": "Soluble extracts of Methylococcus capsulatus (Bath), obtained by centrifugation of crude extracts at 160000g for 1h, catalyse the NAD(P)H- and O2-dependent disappearance of bromomethane, and also the formation of methanol from methane. Soluble methane mono-oxygenase is not inhibited by chelating agents or by most electron-transport inhibitors, and is a multicomponent enzyme.", "contents": "Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath. Soluble extracts of Methylococcus capsulatus (Bath), obtained by centrifugation of crude extracts at 160000g for 1h, catalyse the NAD(P)H- and O2-dependent disappearance of bromomethane, and also the formation of methanol from methane. Soluble methane mono-oxygenase is not inhibited by chelating agents or by most electron-transport inhibitors, and is a multicomponent enzyme.", "PMID": 962879}
{"id": "pubmed23n0032_5278", "title": "Interaction of pepstatin with pig pepsinogen.", "content": "In contrast with pepsin, pepsinogen does not bind pepstatin at pH values between 5.3 and 2.5. Pepsinogen is not retarded by pepstatin immobilized on to aminohexyl-Sepharose at pH5.3 or 4.1, whereas at pH3.0 activation takes place during the chromatography, with retardation of the resultant pepsin.", "contents": "Interaction of pepstatin with pig pepsinogen. In contrast with pepsin, pepsinogen does not bind pepstatin at pH values between 5.3 and 2.5. Pepsinogen is not retarded by pepstatin immobilized on to aminohexyl-Sepharose at pH5.3 or 4.1, whereas at pH3.0 activation takes place during the chromatography, with retardation of the resultant pepsin.", "PMID": 962880}
{"id": "pubmed23n0032_5279", "title": "Instability of rat liver chromatin and other nuclear non-histone proteins in alkaline solution.", "content": "Non-histone proteins from rat liver nuclei and chromatin were shown to be hydrolysed in 0.1M or-1M-NaOH solutions both at 4 degrees and 18 degrees C; 24h in 1M-NaOH at 18 degrees C is sufficient to break down approx. 77% of these proteins to low-molecular-weight peptides. Loss of protein material banding in the region of pH5.5-8.0 has been demonstrated by isoelectric focusing in polyacrylamide gels, and fine high-molecular-weight bands are no longer visible on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The results indicate that care must be taken when analysing non-histone-protein fractions to avoid exposure to alkaline pH conditions.", "contents": "Instability of rat liver chromatin and other nuclear non-histone proteins in alkaline solution. Non-histone proteins from rat liver nuclei and chromatin were shown to be hydrolysed in 0.1M or-1M-NaOH solutions both at 4 degrees and 18 degrees C; 24h in 1M-NaOH at 18 degrees C is sufficient to break down approx. 77% of these proteins to low-molecular-weight peptides. Loss of protein material banding in the region of pH5.5-8.0 has been demonstrated by isoelectric focusing in polyacrylamide gels, and fine high-molecular-weight bands are no longer visible on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The results indicate that care must be taken when analysing non-histone-protein fractions to avoid exposure to alkaline pH conditions.", "PMID": 962881}
{"id": "pubmed23n0032_5280", "title": "The 3H-labelling of bilirubin.", "content": "A simple method has been developed for the preparation of 3H-labelled bilirubin IX-alpha from bilirubin dimethyl ester. The label is located in the propionic acid side chains, and there is no isomerization of the bilirubin during the preparation.", "contents": "The 3H-labelling of bilirubin. A simple method has been developed for the preparation of 3H-labelled bilirubin IX-alpha from bilirubin dimethyl ester. The label is located in the propionic acid side chains, and there is no isomerization of the bilirubin during the preparation.", "PMID": 962882}
{"id": "pubmed23n0032_5281", "title": "Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats.", "content": "The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.", "contents": "Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats. The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.", "PMID": 962883}
{"id": "pubmed23n0032_5282", "title": "Radioimmunological identification of tropoelastin.", "content": "Antiserum was prepared in sheep against insoluble elastin isolated from embryonic-chick aortae. In an indirect immunoprecipitation test, the antiserum reacted quantitatively with small amounts of radioactively labelled purified tropoelastin prepared from embryonic-chick aortae. The antiserum did not cross-react with chick procollagen, and the antiserum uas used to identify radioactively labelled tropoelastin secreted by chick aorta cells in suspension culture.", "contents": "Radioimmunological identification of tropoelastin. Antiserum was prepared in sheep against insoluble elastin isolated from embryonic-chick aortae. In an indirect immunoprecipitation test, the antiserum reacted quantitatively with small amounts of radioactively labelled purified tropoelastin prepared from embryonic-chick aortae. The antiserum did not cross-react with chick procollagen, and the antiserum uas used to identify radioactively labelled tropoelastin secreted by chick aorta cells in suspension culture.", "PMID": 962884}
{"id": "pubmed23n0032_5283", "title": "Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin.", "content": "1. The regulation of the synthesis of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) in epididymal adipose tissue, liver and kidney in vivo was studied immunochemically. 2. Phosphoenolpyruvate carboxykinase (GTP) synthesis in adipose tissue is increased by starvation, diabetes and noradrenaline, and decreased by re-feeding and insulin. These changes were also seen in adrenalectomized rats and are qualitatively similar to those observed for the liver enzyme. This indicates the involvement of cyclic AMP as an inducer and insulin as a de-inducer in the regulation of phosphoenolpyruvate carboxykinase (GTP) in both tissues. (Induction and de-induction are defined as selective increase and decrease respectively in the rate of enzyme synthesis, regardless of the mechanism involved.)3. Adrenalectomy had little effect on phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney, but increased the synthesis rate of the adipose-tissue enzyme. Starvation and adrenalectomy had additive effects in increasing the synthesis rate of adipose-tissue phosphoenolpyruvate carboxykinase (GTP). In adrenalectomized diabetic rats glucocorticoids increased phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney while decreasing enzyme synthesis in adipose tissue. De-induction of adipose tissue phosphoenolpyruvate carboxykinase (GTP) is therefore regulated independently by glucocorticoids and insulin. 4. Although liver, kidney and adipose-tissue phosphoenolpyruvate carboxykinases (GTP) are seemingly identical, there is an apparent tissue-specific differentiation in regulatory systems for the enzyme.", "contents": "Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin. 1. The regulation of the synthesis of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) in epididymal adipose tissue, liver and kidney in vivo was studied immunochemically. 2. Phosphoenolpyruvate carboxykinase (GTP) synthesis in adipose tissue is increased by starvation, diabetes and noradrenaline, and decreased by re-feeding and insulin. These changes were also seen in adrenalectomized rats and are qualitatively similar to those observed for the liver enzyme. This indicates the involvement of cyclic AMP as an inducer and insulin as a de-inducer in the regulation of phosphoenolpyruvate carboxykinase (GTP) in both tissues. (Induction and de-induction are defined as selective increase and decrease respectively in the rate of enzyme synthesis, regardless of the mechanism involved.)3. Adrenalectomy had little effect on phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney, but increased the synthesis rate of the adipose-tissue enzyme. Starvation and adrenalectomy had additive effects in increasing the synthesis rate of adipose-tissue phosphoenolpyruvate carboxykinase (GTP). In adrenalectomized diabetic rats glucocorticoids increased phosphoenolpyruvate carboxykinase (GTP) synthesis in liver and kidney while decreasing enzyme synthesis in adipose tissue. De-induction of adipose tissue phosphoenolpyruvate carboxykinase (GTP) is therefore regulated independently by glucocorticoids and insulin. 4. Although liver, kidney and adipose-tissue phosphoenolpyruvate carboxykinases (GTP) are seemingly identical, there is an apparent tissue-specific differentiation in regulatory systems for the enzyme.", "PMID": 962885}
{"id": "pubmed23n0032_5284", "title": "The effect of cobalt on the uptake of plasma iron by the liver.", "content": "1. After an intraperitoneal injection of 59Fe the recovery of radioactivity in the liver, but not in other tissues, was increased in cobalt-pretreated rats. There was no proportional increase in the radioactivity recovered from liver haem. 2. Rats were injected intravenously with serum containing protein-bound 59Fe and 125I-labelled albumin as a marker. At various times after injection the specific radioactivities of iron in plasma and of non-haem iron in liver were determined; corrections were applied for the content of plasma in samples of liver. In cobalt-pretreated rats there was a more rapid loss of 59Fe radioactivity from the plasma and a corresponding increase in the uptake of 59Fe into liver non-haem iron. 3. The results are discussed in relation to the possible sites of action of cobalt, and the possibility is considered that only a fraction of the liver non-haem iron may be involved.", "contents": "The effect of cobalt on the uptake of plasma iron by the liver. 1. After an intraperitoneal injection of 59Fe the recovery of radioactivity in the liver, but not in other tissues, was increased in cobalt-pretreated rats. There was no proportional increase in the radioactivity recovered from liver haem. 2. Rats were injected intravenously with serum containing protein-bound 59Fe and 125I-labelled albumin as a marker. At various times after injection the specific radioactivities of iron in plasma and of non-haem iron in liver were determined; corrections were applied for the content of plasma in samples of liver. In cobalt-pretreated rats there was a more rapid loss of 59Fe radioactivity from the plasma and a corresponding increase in the uptake of 59Fe into liver non-haem iron. 3. The results are discussed in relation to the possible sites of action of cobalt, and the possibility is considered that only a fraction of the liver non-haem iron may be involved.", "PMID": 962886}
{"id": "pubmed23n0032_5285", "title": "Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate.", "content": "1. The photochemical generation of excited states of oxygen in liver cell culture by the mild ilumination of culture medium containing riboflavin, results in stimulation of benzo[a]pyrene 3-mono-oxygenase, a cytochrome P-450-linked mono-oxygenase. 2. The same large increase in mono-oxygenase activity was found when medium containing riboflavin was illuminated in the absence of cells and then stored in the dark for 24h before contact with the cells. From this it may be inferred that stimulation is due to the formation of a stable inducer in the culture medium. Further experiments indicate that the stable inducer is due to the photo-oxidation of an amino acid. 3. Evidence that singlet oxygen is responsible for initiating the stimulation of the mono-oxygenase is based on the use of molecules that scavenge particular active oxygen species. Of all the scavengers tested, only those that scavenge single oxygen inhibited the stimulation. 4. A hypothesis is developed to relate the stimulation of the mono-oxygenase by singlet oxygen in cultured cells to the regulation of the cytochrome P-450 enzyme system in vivo. It is suggested that single oxygen generation within cells may be a common factor linking the many structurally diverse inducers of the enzyme system.", "contents": "Induction of benzo[a]pyrene Mono-oxygenase in liver cell culture by the photochemical generation of active oxygen species. Evidence for the involvement of singlet oxygen and the formation of a stable inducing intermediate. 1. The photochemical generation of excited states of oxygen in liver cell culture by the mild ilumination of culture medium containing riboflavin, results in stimulation of benzo[a]pyrene 3-mono-oxygenase, a cytochrome P-450-linked mono-oxygenase. 2. The same large increase in mono-oxygenase activity was found when medium containing riboflavin was illuminated in the absence of cells and then stored in the dark for 24h before contact with the cells. From this it may be inferred that stimulation is due to the formation of a stable inducer in the culture medium. Further experiments indicate that the stable inducer is due to the photo-oxidation of an amino acid. 3. Evidence that singlet oxygen is responsible for initiating the stimulation of the mono-oxygenase is based on the use of molecules that scavenge particular active oxygen species. Of all the scavengers tested, only those that scavenge single oxygen inhibited the stimulation. 4. A hypothesis is developed to relate the stimulation of the mono-oxygenase by singlet oxygen in cultured cells to the regulation of the cytochrome P-450 enzyme system in vivo. It is suggested that single oxygen generation within cells may be a common factor linking the many structurally diverse inducers of the enzyme system.", "PMID": 962887}
{"id": "pubmed23n0032_5286", "title": "Anti-ketogenic effect of glucose in the lactating cow deprived of food.", "content": "1. The aim of this study was to determine the effect of a constant infusion of glucose on the ketosis that is observed when dairy cows are deprived of food in early lactation. 2. Cows in early lactation were first deprived of food for 4 days (96h) to induce a 'fasting ketosis'. Glucose was then infused intravenously at a constant rate of 0.75 g/min for 48h while deprivation of food was maintained. At the end of this 48 h period, blood and liver ketone-body concentrations had decreased to values well below those found in healthy fed cows. 3. On the assumption that the anti-ketogenic effect of glucose was mainly due to suppression of hepatic ketogenesis, it was concluded that two anti-ketogenic mechanisms had been identified. These were (a) a decrease in the availability of free fatty acids for hepatic oxidation, and (b) anti-ketogenic changes within the liver itself. 4. These latter anti-ketogenic changes were twofold. The first was a major increase in the hepatic concentrations of citrate and 2-oxoglutarate. The second was an increase in the degree of oxidation of the hepatic cytosol. It was proposed that both these intrahepatic changes might indicate an augmentation of the quantity of oxaloacetate available for condensation with acetyl-CoA derived from fat oxidation. 5. Hepatic glycerol 1-phosphate concentration fell substantially after glucose infusion. 6. Glucose infusion into fed cows produced qualitatively similar effects to those observed in the unfed cows. However, blood and liver ketone-body concentrations were not decreased to the same extent in the fed cows as in the unfed cows.", "contents": "Anti-ketogenic effect of glucose in the lactating cow deprived of food. 1. The aim of this study was to determine the effect of a constant infusion of glucose on the ketosis that is observed when dairy cows are deprived of food in early lactation. 2. Cows in early lactation were first deprived of food for 4 days (96h) to induce a 'fasting ketosis'. Glucose was then infused intravenously at a constant rate of 0.75 g/min for 48h while deprivation of food was maintained. At the end of this 48 h period, blood and liver ketone-body concentrations had decreased to values well below those found in healthy fed cows. 3. On the assumption that the anti-ketogenic effect of glucose was mainly due to suppression of hepatic ketogenesis, it was concluded that two anti-ketogenic mechanisms had been identified. These were (a) a decrease in the availability of free fatty acids for hepatic oxidation, and (b) anti-ketogenic changes within the liver itself. 4. These latter anti-ketogenic changes were twofold. The first was a major increase in the hepatic concentrations of citrate and 2-oxoglutarate. The second was an increase in the degree of oxidation of the hepatic cytosol. It was proposed that both these intrahepatic changes might indicate an augmentation of the quantity of oxaloacetate available for condensation with acetyl-CoA derived from fat oxidation. 5. Hepatic glycerol 1-phosphate concentration fell substantially after glucose infusion. 6. Glucose infusion into fed cows produced qualitatively similar effects to those observed in the unfed cows. However, blood and liver ketone-body concentrations were not decreased to the same extent in the fed cows as in the unfed cows.", "PMID": 962888}
{"id": "pubmed23n0032_5287", "title": "The metabolism and binding of catecholamines by the hepatic microsomal mixed-function oxidase of the rat.", "content": "Noradrenaline and adrenaline were metabolized by an NADPH- and oxygen-dependent process located within the hepatic microsomal fraction of the rat. Metabolism was inhibited by CO and compound SKF 525A, but not by pargyline, an inhibitor of monoamine oxidase, or by 3,4-dimethoxy-5-hydroxybenzoic acid, an inhibitor of catechol O-methyltransferase. It is concluded that the enzyme system responsible for the metabolism of the catecholamines was the microsomal mixed-function oxidase. The Km for noradrenaline was 2.4 mM and for adrenaline 1.0 mM, and V 15.6 and 3.6 nmol/min per mg of microsomal protein respectively. Both catecholamines bound to the microsomal fraction, producing a type II spectral change, with a Ks for noradrenaline of 0.9 mM and for adrenaline of 1.0 mM, and showed other characteristics of type II compounds by inhibited the reduction of cytochrome P-450 by NADPH and exhibiting an enhanced metabolism in the presence of acetone. The major product of catecholamine metabolism was an as yet unidentified alkali-labile compound, which did not correspond to any of the recognized catecholamine metabolites.", "contents": "The metabolism and binding of catecholamines by the hepatic microsomal mixed-function oxidase of the rat. Noradrenaline and adrenaline were metabolized by an NADPH- and oxygen-dependent process located within the hepatic microsomal fraction of the rat. Metabolism was inhibited by CO and compound SKF 525A, but not by pargyline, an inhibitor of monoamine oxidase, or by 3,4-dimethoxy-5-hydroxybenzoic acid, an inhibitor of catechol O-methyltransferase. It is concluded that the enzyme system responsible for the metabolism of the catecholamines was the microsomal mixed-function oxidase. The Km for noradrenaline was 2.4 mM and for adrenaline 1.0 mM, and V 15.6 and 3.6 nmol/min per mg of microsomal protein respectively. Both catecholamines bound to the microsomal fraction, producing a type II spectral change, with a Ks for noradrenaline of 0.9 mM and for adrenaline of 1.0 mM, and showed other characteristics of type II compounds by inhibited the reduction of cytochrome P-450 by NADPH and exhibiting an enhanced metabolism in the presence of acetone. The major product of catecholamine metabolism was an as yet unidentified alkali-labile compound, which did not correspond to any of the recognized catecholamine metabolites.", "PMID": 962889}
{"id": "pubmed23n0032_5288", "title": "Serum glycoprotein synthesis after partial hepatectomy in the rat.", "content": "The incorporation in vivo of D-[1-14C]glucosamine into serum glycoproteins and proteins of liver microsomal fractions shows a decrease in the early stages (24h) after partial hepatectomy compared with sham-operated animals; 72h after partial hepatectomy the specific radioactivity of hexosamines bound to liver microsomal fractions reaches the same value as for sham-operated animals.", "contents": "Serum glycoprotein synthesis after partial hepatectomy in the rat. The incorporation in vivo of D-[1-14C]glucosamine into serum glycoproteins and proteins of liver microsomal fractions shows a decrease in the early stages (24h) after partial hepatectomy compared with sham-operated animals; 72h after partial hepatectomy the specific radioactivity of hexosamines bound to liver microsomal fractions reaches the same value as for sham-operated animals.", "PMID": 962890}
{"id": "pubmed23n0032_5289", "title": "Animal liver tryptophan pyrrolases: Absence of apoenzyme and of hormonal induction mechanism from species sensitive to tryptophan toxicity.", "content": "1. Liver tryptophan pyrrolase exists as holoenzyme and apoenzyme in rat, mouse, pig, turkey, chicken and possibly man. 2. The apoenzyme is absent from cat, frog, gerbil, guinea pig, hamster, ox, sheep and rabbit. 3. The hormonal mechanism of induction of the pyrrolase is absent from species lacking the apoenzyme. 4. The concentrations of tryptophan in livers and sera of these species are lower than in species possessing the apoenzyme. 5. Species lacking the apoenzyme or the hormonal induction mechanism have a deficient kynurenine pathway and are sensitive to the toxicity of tryptophan. 6. It is suggested that these species are not suitable as models for studying human tryptophan metabolism. 7. The possible significance of these findings in relation to veterinary and human neonatal care is discussed.", "contents": "Animal liver tryptophan pyrrolases: Absence of apoenzyme and of hormonal induction mechanism from species sensitive to tryptophan toxicity. 1. Liver tryptophan pyrrolase exists as holoenzyme and apoenzyme in rat, mouse, pig, turkey, chicken and possibly man. 2. The apoenzyme is absent from cat, frog, gerbil, guinea pig, hamster, ox, sheep and rabbit. 3. The hormonal mechanism of induction of the pyrrolase is absent from species lacking the apoenzyme. 4. The concentrations of tryptophan in livers and sera of these species are lower than in species possessing the apoenzyme. 5. Species lacking the apoenzyme or the hormonal induction mechanism have a deficient kynurenine pathway and are sensitive to the toxicity of tryptophan. 6. It is suggested that these species are not suitable as models for studying human tryptophan metabolism. 7. The possible significance of these findings in relation to veterinary and human neonatal care is discussed.", "PMID": 962891}
{"id": "pubmed23n0032_5290", "title": "The metabolism of steroids in the fatty liver induced by orotic acid feeding.", "content": "1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5alpha-[4-14C]androstane-3alpha,17beta-diol, [4-14C]cholesterol, 7alpha-hydroxy-4-[6beta-3H]cholesten-3-one, 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 6beta- and 7alpha-hydroxylation of 4-androstene3,17-dione, and the 2alpha-, 2beta- and 18-hydroxylation of 5alpha-androstane-3alpha,17beta-diol, and the 5alpha-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40--50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one was decreased by about 50%, whereas the 7alpha-hydroxylation of cholesterol and the 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol were not significantly decreased. The 6beta-hydroxylation of lithocholic acid was stimulated by 40%. 5. The results are discussed in relation to present knowledge of the heapatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.", "contents": "The metabolism of steroids in the fatty liver induced by orotic acid feeding. 1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5alpha-[4-14C]androstane-3alpha,17beta-diol, [4-14C]cholesterol, 7alpha-hydroxy-4-[6beta-3H]cholesten-3-one, 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 6beta- and 7alpha-hydroxylation of 4-androstene3,17-dione, and the 2alpha-, 2beta- and 18-hydroxylation of 5alpha-androstane-3alpha,17beta-diol, and the 5alpha-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40--50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one was decreased by about 50%, whereas the 7alpha-hydroxylation of cholesterol and the 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol were not significantly decreased. The 6beta-hydroxylation of lithocholic acid was stimulated by 40%. 5. The results are discussed in relation to present knowledge of the heapatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.", "PMID": 962892}
{"id": "pubmed23n0032_5291", "title": "The effect of insulin and glucocorticoids on the synthesis and degradation of phosphoenolpyruvate carboxykinase (GTP) in rat adipose tissue cultured in vitro.", "content": "1. Epididymal adipose tissue from the rat was maintained in culture for periods of up to 96h. 2. After an initial decrease in protein synthesis during the first 24h of culture, the adipose tissue recovered its capacity to synthesize and accumulate proteins of a relatively large size. 3. The activity of phosphoenolpyruvate carboxykinase decreased in a parallel manner, but increased again after 24h of incubation of the tissue in culture, to a value twice that noted in the tissue in vivo. This increase in enzyme activity was due to an increase in its rate of synthesis. 4. Both insulin and dexamethasone (9alpha-fluoro-16alpha-methyl-11beta,17,-21-trihydroxypregna-1,4-diene-3,20-dione) inhibited phosphoenolpyruvate carboxykinase synthesis, but dexamethasone also decreased total protein synthesis. 5. The half-life of phosphoenolpyruvate carboxykinase in adipose tissue cultured in vitro was 5--7h and was not altered by insulin or dexamethasone. 6. It is concluded that both insulin and glucocroticoids lower the activity of phosphoenolpyruvate carboxykinase in rat adipose tissue by decreasing its rate of synthesis.", "contents": "The effect of insulin and glucocorticoids on the synthesis and degradation of phosphoenolpyruvate carboxykinase (GTP) in rat adipose tissue cultured in vitro. 1. Epididymal adipose tissue from the rat was maintained in culture for periods of up to 96h. 2. After an initial decrease in protein synthesis during the first 24h of culture, the adipose tissue recovered its capacity to synthesize and accumulate proteins of a relatively large size. 3. The activity of phosphoenolpyruvate carboxykinase decreased in a parallel manner, but increased again after 24h of incubation of the tissue in culture, to a value twice that noted in the tissue in vivo. This increase in enzyme activity was due to an increase in its rate of synthesis. 4. Both insulin and dexamethasone (9alpha-fluoro-16alpha-methyl-11beta,17,-21-trihydroxypregna-1,4-diene-3,20-dione) inhibited phosphoenolpyruvate carboxykinase synthesis, but dexamethasone also decreased total protein synthesis. 5. The half-life of phosphoenolpyruvate carboxykinase in adipose tissue cultured in vitro was 5--7h and was not altered by insulin or dexamethasone. 6. It is concluded that both insulin and glucocroticoids lower the activity of phosphoenolpyruvate carboxykinase in rat adipose tissue by decreasing its rate of synthesis.", "PMID": 962893}
{"id": "pubmed23n0032_5292", "title": "Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.", "content": "Heterogeneity among the free small ribosomal subunits of rat liver was studied. Newly made free small subunits differ from the bulk of the free subunits in the following ways. (1) The newly made free small subunits sediment slightly faster than most free small subunits and form a band at slightly lower densities. (2) Mature free small subunits, prepared under conditions which minimize the amount of loosely bound protein, readily dimerize in solutions of low ionic strength. Little dimerization is found with newly formed subunits. This permits the separation of fractions enriched in newly formed subunits. (3) The fraction enriched in newly formed subunits contains a distinctive additional protein as compared the most of the free small subunits.", "contents": "Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm. Heterogeneity among the free small ribosomal subunits of rat liver was studied. Newly made free small subunits differ from the bulk of the free subunits in the following ways. (1) The newly made free small subunits sediment slightly faster than most free small subunits and form a band at slightly lower densities. (2) Mature free small subunits, prepared under conditions which minimize the amount of loosely bound protein, readily dimerize in solutions of low ionic strength. Little dimerization is found with newly formed subunits. This permits the separation of fractions enriched in newly formed subunits. (3) The fraction enriched in newly formed subunits contains a distinctive additional protein as compared the most of the free small subunits.", "PMID": 962894}
{"id": "pubmed23n0032_5293", "title": "Association of Sj\u00f6gren's syndrome with HLA-B8.", "content": "HLA antigens were examined by microdroplet lymphocyte cytotoxicity in 31 patients with Sj\u00f6grens syndrome. Fifty percent of 22 Causcasian patients (7 of 17 females and 4 of 5 males) had HLA-B8, as compared to 21% of 1205 controls (P=0.002). The presence of HLA-B8 was not correlated with parotid salivary flow, keratoconjunctivitis sicca, or focus score. Because of its association with a number of autoimmune diseases, including Sj\u00f6gren's syndrome, it is suggested that HLA-B8 is genetically liked to an immune response gene(s) that predisposes the individual to autoimmune phenomena. The expression of autoimmunity may be determined by infectious or environmental factors.", "contents": "Association of Sj\u00f6gren's syndrome with HLA-B8. HLA antigens were examined by microdroplet lymphocyte cytotoxicity in 31 patients with Sj\u00f6grens syndrome. Fifty percent of 22 Causcasian patients (7 of 17 females and 4 of 5 males) had HLA-B8, as compared to 21% of 1205 controls (P=0.002). The presence of HLA-B8 was not correlated with parotid salivary flow, keratoconjunctivitis sicca, or focus score. Because of its association with a number of autoimmune diseases, including Sj\u00f6gren's syndrome, it is suggested that HLA-B8 is genetically liked to an immune response gene(s) that predisposes the individual to autoimmune phenomena. The expression of autoimmunity may be determined by infectious or environmental factors.", "PMID": 962969}
{"id": "pubmed23n0032_5294", "title": "Rheumatoid factor in acute bacterial endocarditis.", "content": "Sera from 55 parenteral drug abusers with endocarditis due to Staphylococcus aureus were assayed for the presence and titer of rheumatoid factor. Thirteen (24%) of the 55 patients with endocarditis had sera positive for rheumatoid factor at one point or another in their courses; only 2 (7%) of 30 noninfected drug users were found to be positive. It appeared that more severe cases, as evidenced by duration of fever after initiation of antibiotic therapy, were more likely to develop rheumatoid factor.", "contents": "Rheumatoid factor in acute bacterial endocarditis. Sera from 55 parenteral drug abusers with endocarditis due to Staphylococcus aureus were assayed for the presence and titer of rheumatoid factor. Thirteen (24%) of the 55 patients with endocarditis had sera positive for rheumatoid factor at one point or another in their courses; only 2 (7%) of 30 noninfected drug users were found to be positive. It appeared that more severe cases, as evidenced by duration of fever after initiation of antibiotic therapy, were more likely to develop rheumatoid factor.", "PMID": 962970}
{"id": "pubmed23n0032_5295", "title": "Connective tissue synthesis by cultured scleroderma fibroblasts. I. In vitro collagen synthesis by normal and scleroderma dermal fibroblasts.", "content": "The authors have been unable to demonstrate an increase in collagen synthesis by fibroblasts isolated from  sclerodermatous skin. In order to elucidate this problem further, scleroderma fibroblasts were biopsied from upper dermis, from lower (including subcutaneous) dermis, and from adjacent clinically noninvolved skin. All cell lines failed to show a significant increase in collagen synthesis when they were compared to control fibroblast lines. One difference among them was that fibroblasts from involved areas showed a rate of collagen synthesis equal to or less than cells isolated from adjacent clinically noninvolved sites.", "contents": "Connective tissue synthesis by cultured scleroderma fibroblasts. I. In vitro collagen synthesis by normal and scleroderma dermal fibroblasts. The authors have been unable to demonstrate an increase in collagen synthesis by fibroblasts isolated from  sclerodermatous skin. In order to elucidate this problem further, scleroderma fibroblasts were biopsied from upper dermis, from lower (including subcutaneous) dermis, and from adjacent clinically noninvolved skin. All cell lines failed to show a significant increase in collagen synthesis when they were compared to control fibroblast lines. One difference among them was that fibroblasts from involved areas showed a rate of collagen synthesis equal to or less than cells isolated from adjacent clinically noninvolved sites.", "PMID": 962971}
{"id": "pubmed23n0032_5296", "title": "Fibrous myopathy. A rheumatic complication of drug abuse.", "content": "A progressive fibrous myopathy may result from chronic intramuscular drug abuse. This complication may mimic other rheumatic disorders and early recognition may prevent disability. The patient described here presented with fixed flexion and extension contracture of hips and knees, respectively, after abusing meperidine and other agents for 3 years. Soft tissues of thighs and buttocks were \"wood hard,\" EMG showed absence of action potentials in affected muscles, and biopsy revealed extensive replacement of muscle with dense, acellular fibrous tissue. Possible mechanisms are discussed.", "contents": "Fibrous myopathy. A rheumatic complication of drug abuse. A progressive fibrous myopathy may result from chronic intramuscular drug abuse. This complication may mimic other rheumatic disorders and early recognition may prevent disability. The patient described here presented with fixed flexion and extension contracture of hips and knees, respectively, after abusing meperidine and other agents for 3 years. Soft tissues of thighs and buttocks were \"wood hard,\" EMG showed absence of action potentials in affected muscles, and biopsy revealed extensive replacement of muscle with dense, acellular fibrous tissue. Possible mechanisms are discussed.", "PMID": 962972}
{"id": "pubmed23n0032_5297", "title": "Intracellular distribution of radiogold. Localization to large granule membranes.", "content": "The distribution of gold in Kupffer cells and in subcellular fractions of rat liver was studied at intervals following intraperitoneal injection of 195Au sodium thiomalate (Myochrysine). Kupffer cells, isolated by digestion of whole liver with Pronase, had radioactive gold counts per milligram of protein that were twice the counts in the digested liver supernatant. After fractionation of liver cells by differential centrifugation, radiogold was found predominantly in the nuclear, mitochondrial, and lysosomal fractions. When the distribution of isotope was related to the protein content, the highest gold concentration was found in the lysosomal fraction, where it was 28 times that in the soluble fraction. Most radiogold was nondialyzable, probably a result of binding to larger intracellular compounds. Approximately 95% of the nondialyzable gold was in the organelle membrane of attached to membrane-adsorbed material, as determined by treatment of dialyzed mitochondrial and lysosomal fractions with Triton X-100. These data suggest that the intracellular locus of gold action may reside in organelle membranes.", "contents": "Intracellular distribution of radiogold. Localization to large granule membranes. The distribution of gold in Kupffer cells and in subcellular fractions of rat liver was studied at intervals following intraperitoneal injection of 195Au sodium thiomalate (Myochrysine). Kupffer cells, isolated by digestion of whole liver with Pronase, had radioactive gold counts per milligram of protein that were twice the counts in the digested liver supernatant. After fractionation of liver cells by differential centrifugation, radiogold was found predominantly in the nuclear, mitochondrial, and lysosomal fractions. When the distribution of isotope was related to the protein content, the highest gold concentration was found in the lysosomal fraction, where it was 28 times that in the soluble fraction. Most radiogold was nondialyzable, probably a result of binding to larger intracellular compounds. Approximately 95% of the nondialyzable gold was in the organelle membrane of attached to membrane-adsorbed material, as determined by treatment of dialyzed mitochondrial and lysosomal fractions with Triton X-100. These data suggest that the intracellular locus of gold action may reside in organelle membranes.", "PMID": 962973}
{"id": "pubmed23n0032_5298", "title": "Carpo:metacarpal ratio. A new quantitative measure of radiologic progression of wrist involvement in rheumatioid arthritis.", "content": "Analysis of 878 hand films shows the carpo : metacarpal ratio to be a sensitive quantitative index of progression of carpal involvement in rheumatoid arthritis. This index, easily calculated by dividing the lengths of the carpus by the third metacarpal, showed marked consistency in the ratio regardless of age, race, or sex in normal adults. A decreased ratio correlated highly with recognized radiographic stages of rheumatoid arthritis and thus suggested utility in longitudinal studies of patients with this disease.", "contents": "Carpo:metacarpal ratio. A new quantitative measure of radiologic progression of wrist involvement in rheumatioid arthritis. Analysis of 878 hand films shows the carpo : metacarpal ratio to be a sensitive quantitative index of progression of carpal involvement in rheumatoid arthritis. This index, easily calculated by dividing the lengths of the carpus by the third metacarpal, showed marked consistency in the ratio regardless of age, race, or sex in normal adults. A decreased ratio correlated highly with recognized radiographic stages of rheumatoid arthritis and thus suggested utility in longitudinal studies of patients with this disease.", "PMID": 962974}
{"id": "pubmed23n0032_5299", "title": "Relationship between lipid composition and antibiotic-resistance to isoniazid, streptomycin, p-aminosalicylic acid, ethambutol, rifampicin in mycobacteria.", "content": "The content of neutral lipids, peptido-glycolipids and phospholipids, separated by solvent extractions and TLC, was investigated and compared in various strains of Mycobacterium tuberculosis and in Mycobacterium avium. The examined strains could be divided into two groups: (i) those possessing a stable INH-resistance with a chromosomal location for the resistance genes, in which the content of total lipids and the distribution of lipid subfractions were modified with respect to the control strain H37Rv; (ii) those strains, such as the mutants resistant to streptomycin (Sm), ethambutol (EM), p-aminosalicylic acid (PAS) and rifampicin (R/AMP), uncorrelated drugs with different genetic location of the antibiotic-resistance, in which a disturbance of the lipid metabolism does not appeared to be significant. TLC and chemical analysis of INH-mutants suggested that the decrease of the cell lipids was associated with the peptidoglyco-phospholipid fraction, while the neutral lipids were characterized by a most elevated amount of both menaquinone-9 and free fatty acids, and by comparable levels of both glycerides and total fatty acids with respect to the sensitive strain. In the pattern of fatty acid shorter than C26 of all the fractions studied, no significant differences were observed between the H37Rv strain and the other resistant strains, including the M. avium. In the course of the lipid analysis no unusual phospholipids in addition to CL, PE and phosphatidylinositol-oligo-mannoside (PIM) were detected. Reasoning in a speculative way, the dramatic fall of the complex lipid elaboration in the INH-resistant mycobacteria, may underline a role for these substances in the envelope of the mycobacterial cell that defends itself from the selecting attack of the antibiotic molecule and that keeps this phenotypic expression of INH-resistance in the mutants.", "contents": "Relationship between lipid composition and antibiotic-resistance to isoniazid, streptomycin, p-aminosalicylic acid, ethambutol, rifampicin in mycobacteria. The content of neutral lipids, peptido-glycolipids and phospholipids, separated by solvent extractions and TLC, was investigated and compared in various strains of Mycobacterium tuberculosis and in Mycobacterium avium. The examined strains could be divided into two groups: (i) those possessing a stable INH-resistance with a chromosomal location for the resistance genes, in which the content of total lipids and the distribution of lipid subfractions were modified with respect to the control strain H37Rv; (ii) those strains, such as the mutants resistant to streptomycin (Sm), ethambutol (EM), p-aminosalicylic acid (PAS) and rifampicin (R/AMP), uncorrelated drugs with different genetic location of the antibiotic-resistance, in which a disturbance of the lipid metabolism does not appeared to be significant. TLC and chemical analysis of INH-mutants suggested that the decrease of the cell lipids was associated with the peptidoglyco-phospholipid fraction, while the neutral lipids were characterized by a most elevated amount of both menaquinone-9 and free fatty acids, and by comparable levels of both glycerides and total fatty acids with respect to the sensitive strain. In the pattern of fatty acid shorter than C26 of all the fractions studied, no significant differences were observed between the H37Rv strain and the other resistant strains, including the M. avium. In the course of the lipid analysis no unusual phospholipids in addition to CL, PE and phosphatidylinositol-oligo-mannoside (PIM) were detected. Reasoning in a speculative way, the dramatic fall of the complex lipid elaboration in the INH-resistant mycobacteria, may underline a role for these substances in the envelope of the mycobacterial cell that defends itself from the selecting attack of the antibiotic molecule and that keeps this phenotypic expression of INH-resistance in the mutants.", "PMID": 962981}
{"id": "pubmed23n0032_5300", "title": "Association of medullary carcinoma of the thyroid with carcinoembryonic antigen.", "content": "To investigate the association between medullary carcinoma of the thyroid (MCT) and carcinoembryonic antigen (CEA), we assayed 78 sera from patients with thyroid diseases for CEA, employing the radioimmunoassay of double antibody technique. All 13 sera from patients with MCT had high levels of CEA, ranging from 14 to 170 ng/ml. Increased serum CEA was noted even in cases of small, localized carcinoma. By contrast, serum CEA levels were normal (below 10 ng/ml) in all other histological types of thyroid carcinoma (33 cases), except for one case of papillary adenocarcinoma. In 32 patients with non-malignant thyroid diseases, with few exceptions serum CEA levels remained within the normal range. The elevated serum levels of CEA in MCT returned to normal after successful operation. Furthermore, very high tissue concentrations of CEA were demonstrated in MCT. The results indicate that CEA is actively produced by MCT, and that its measurement is useful in the diagnosis and management of the disease. It is suggested that the highly specific association of CEA with MCT may well be related to a defect of neural crest origin.", "contents": "Association of medullary carcinoma of the thyroid with carcinoembryonic antigen. To investigate the association between medullary carcinoma of the thyroid (MCT) and carcinoembryonic antigen (CEA), we assayed 78 sera from patients with thyroid diseases for CEA, employing the radioimmunoassay of double antibody technique. All 13 sera from patients with MCT had high levels of CEA, ranging from 14 to 170 ng/ml. Increased serum CEA was noted even in cases of small, localized carcinoma. By contrast, serum CEA levels were normal (below 10 ng/ml) in all other histological types of thyroid carcinoma (33 cases), except for one case of papillary adenocarcinoma. In 32 patients with non-malignant thyroid diseases, with few exceptions serum CEA levels remained within the normal range. The elevated serum levels of CEA in MCT returned to normal after successful operation. Furthermore, very high tissue concentrations of CEA were demonstrated in MCT. The results indicate that CEA is actively produced by MCT, and that its measurement is useful in the diagnosis and management of the disease. It is suggested that the highly specific association of CEA with MCT may well be related to a defect of neural crest origin.", "PMID": 962990}
{"id": "pubmed23n0032_5301", "title": "Anti-tumour immunity in malignant melanoma assay by tube leucocyte adherence inhibition.", "content": "Tumour antigen-induced inhibition of leucocyte adherence was modified for use in glass test tubes (Tube LAI assay) for the study of cell-mediated anti-tumour immunity to human malignant melanoma. Peripheral blood leucocytes (PBL) of 20 out of 25 patients (80%) with active malignant melanoma responded to an extract of malignant melanoma with LAI, whereas only 4-5% of 475 control subjects showed a response. The malignant melanoma patients reacted to both allogeneic and autologous extracts of malignant melanoma which indicates a common cross-reacting antigen. Malignant melanoma patients did not respond to unrelated tumour extracts. The LAI was mediated by PBL (monocytes) \"armed\" with cytophilic anti-tumour antibody specific for the sensitizing tumour antigen. The anti-tumour response of the malignant melanoma patients was dependent on the stage of the cancer, and 11 out of 13 Stage I patients had a positive NAI,  whereas patients with disseminated cancer had decreased response. The diminished LAI in patients with large tumour burdens appeared to be the result of release of tumour antigen systemically. Also, surgery and chemotherapy depressed LAI. Although LAI was depressed after surgical excision of the cutaneous melanoma, most patients showed LAI 1-3 months later. Tumour-free melanoma patients monitored for one year by the Tube LAI assay showed a decline in their anti-tumour immunity 5-6 months after surgery. The NAI was low or negative after the 8th post-surgical month in tumour-free patients. Patients with residual malignant melanoma showed persistent or  recurrent LAI after the 8th post-surgical month. LAI reactivity monitored after \"curative\" surgery for malignant melanoma may assist in determining whether the patient is tumour-free or has a recurrence.", "contents": "Anti-tumour immunity in malignant melanoma assay by tube leucocyte adherence inhibition. Tumour antigen-induced inhibition of leucocyte adherence was modified for use in glass test tubes (Tube LAI assay) for the study of cell-mediated anti-tumour immunity to human malignant melanoma. Peripheral blood leucocytes (PBL) of 20 out of 25 patients (80%) with active malignant melanoma responded to an extract of malignant melanoma with LAI, whereas only 4-5% of 475 control subjects showed a response. The malignant melanoma patients reacted to both allogeneic and autologous extracts of malignant melanoma which indicates a common cross-reacting antigen. Malignant melanoma patients did not respond to unrelated tumour extracts. The LAI was mediated by PBL (monocytes) \"armed\" with cytophilic anti-tumour antibody specific for the sensitizing tumour antigen. The anti-tumour response of the malignant melanoma patients was dependent on the stage of the cancer, and 11 out of 13 Stage I patients had a positive NAI,  whereas patients with disseminated cancer had decreased response. The diminished LAI in patients with large tumour burdens appeared to be the result of release of tumour antigen systemically. Also, surgery and chemotherapy depressed LAI. Although LAI was depressed after surgical excision of the cutaneous melanoma, most patients showed LAI 1-3 months later. Tumour-free melanoma patients monitored for one year by the Tube LAI assay showed a decline in their anti-tumour immunity 5-6 months after surgery. The NAI was low or negative after the 8th post-surgical month in tumour-free patients. Patients with residual malignant melanoma showed persistent or  recurrent LAI after the 8th post-surgical month. LAI reactivity monitored after \"curative\" surgery for malignant melanoma may assist in determining whether the patient is tumour-free or has a recurrence.", "PMID": 962991}
{"id": "pubmed23n0032_5302", "title": "Chemical transformation of Chinese hamster cells. I. A comparison of some properties of transformed cells.", "content": "Fifty-one subclones from carcinogen-treated cells of 3 tissues (kidney, liver and prostate) of the male Chinese hamster have been studied to determine the relationships of 3 criteria of in vitro transformation: morphological change, increased plating efficiency and growth in soft agar. There was no correlation between increased plating efficiency and the other 2 parameters. Morphological change was not always easily recognisable, particularly in cells derived from liver, and was not always a stable feature of any given subclone. This may be due to the technique of isolation used (ring cloning) or may be due to chemically-treated cells requiring long periods of culturing before attaining a stable phenotype. When a stable morphological appearance was achieved, there was good correlation between transformed morphology and colony formation in soft agar. The problems of scoring morphological change as an assessment of malignant transformation, and the importance of spontaneous morphological changes are discussed.", "contents": "Chemical transformation of Chinese hamster cells. I. A comparison of some properties of transformed cells. Fifty-one subclones from carcinogen-treated cells of 3 tissues (kidney, liver and prostate) of the male Chinese hamster have been studied to determine the relationships of 3 criteria of in vitro transformation: morphological change, increased plating efficiency and growth in soft agar. There was no correlation between increased plating efficiency and the other 2 parameters. Morphological change was not always easily recognisable, particularly in cells derived from liver, and was not always a stable feature of any given subclone. This may be due to the technique of isolation used (ring cloning) or may be due to chemically-treated cells requiring long periods of culturing before attaining a stable phenotype. When a stable morphological appearance was achieved, there was good correlation between transformed morphology and colony formation in soft agar. The problems of scoring morphological change as an assessment of malignant transformation, and the importance of spontaneous morphological changes are discussed.", "PMID": 962992}
{"id": "pubmed23n0032_5303", "title": "Serum ferritin concentration in untreated Hodgkin's disease.", "content": "Serum ferritin has been estimated in 125 untreated patients with Hodgkin's disease. Increasing concentrations are found at each advancing stage of the disease and high concentrations are found in patients with systemic symptoms. In all cases this is associated with a low serum Fe concentration and reduced transferrin saturation. There is no relationship between serum ferritin concentration and histological type of disease. The findings are compatible with a non-specific response of the reticuloendothelial system to malignancy, producing a secondary disorder of Fe metabolism.", "contents": "Serum ferritin concentration in untreated Hodgkin's disease. Serum ferritin has been estimated in 125 untreated patients with Hodgkin's disease. Increasing concentrations are found at each advancing stage of the disease and high concentrations are found in patients with systemic symptoms. In all cases this is associated with a low serum Fe concentration and reduced transferrin saturation. There is no relationship between serum ferritin concentration and histological type of disease. The findings are compatible with a non-specific response of the reticuloendothelial system to malignancy, producing a secondary disorder of Fe metabolism.", "PMID": 962993}
{"id": "pubmed23n0032_5304", "title": "Clinical trial of combination chemotherapy and specific active immunotherapy in disseminated melanoma.", "content": "Fifty-six patients with disseminated malignant melanoma were randomly allocated to two treatment groups. The first group C received combination chemotherapy consisting of DTIC and ICRF 159. The second group (C+I) received the same chemotherapy but were also immunized with 2 X 10(7) irradiated allogeneic melanoma cells mixed with 50 mug of percutaneous BCG. The survival rates in both treatment groups C and (C+I) were not significantly different, and only minor enhancement of the chemotherapy was found in the (C+I) group. A similar pattern of tissue response was observed in both groups: lymph node, skin and, to some extent liver metastases, respond better than other sites.", "contents": "Clinical trial of combination chemotherapy and specific active immunotherapy in disseminated melanoma. Fifty-six patients with disseminated malignant melanoma were randomly allocated to two treatment groups. The first group C received combination chemotherapy consisting of DTIC and ICRF 159. The second group (C+I) received the same chemotherapy but were also immunized with 2 X 10(7) irradiated allogeneic melanoma cells mixed with 50 mug of percutaneous BCG. The survival rates in both treatment groups C and (C+I) were not significantly different, and only minor enhancement of the chemotherapy was found in the (C+I) group. A similar pattern of tissue response was observed in both groups: lymph node, skin and, to some extent liver metastases, respond better than other sites.", "PMID": 962994}
{"id": "pubmed23n0032_5305", "title": "Distribution of survival times of 12,000 head and neck cancer patients who died with their disease.", "content": "The lognormal parametric statistical model can provide, for groups of carcinoma cervix patients, good estimates of long-term survival fractions several years earlier than would otherwise be possible. The present paper extends this model work to head and neck cancer by using a minimum chi-squared test for goodness of fit (P greater than 0-05), to study the distribution of survival times of patients who died with their cancer present. Some 12,000 case histories were available from 7 hospital registries, 4 regional cancer registries and one national registry (the OPCS). All histories were followed up for at least 10 years subsequent to treatment and could be grouped into one of 8 cancer sites: antrum, floor of mouth, larynx, nasopharynx, pyriform fossa, post cricoid, tonsil and tongue. The theoretical distributions investigated were the lognormal, negative exponential and skew exponential. The results showed that the lognormal provided the best overall fit to the data, although the range of optimum values for the lognormal parameter, S, differed with cancer site. The optimum range did, however, usually include the value S=0-45. These results will now permit the second stage of validation of the lognormal model to proceed for head and neck cancers.", "contents": "Distribution of survival times of 12,000 head and neck cancer patients who died with their disease. The lognormal parametric statistical model can provide, for groups of carcinoma cervix patients, good estimates of long-term survival fractions several years earlier than would otherwise be possible. The present paper extends this model work to head and neck cancer by using a minimum chi-squared test for goodness of fit (P greater than 0-05), to study the distribution of survival times of patients who died with their cancer present. Some 12,000 case histories were available from 7 hospital registries, 4 regional cancer registries and one national registry (the OPCS). All histories were followed up for at least 10 years subsequent to treatment and could be grouped into one of 8 cancer sites: antrum, floor of mouth, larynx, nasopharynx, pyriform fossa, post cricoid, tonsil and tongue. The theoretical distributions investigated were the lognormal, negative exponential and skew exponential. The results showed that the lognormal provided the best overall fit to the data, although the range of optimum values for the lognormal parameter, S, differed with cancer site. The optimum range did, however, usually include the value S=0-45. These results will now permit the second stage of validation of the lognormal model to proceed for head and neck cancers.", "PMID": 962995}
{"id": "pubmed23n0032_5306", "title": "Faecal steroids and bacteria and large bowel cancer in Hong Kong by socio-economic groups.", "content": "In a study of three socio-economic groups in Hong Kong, the high income group had a high faecal concentration of bile acids, especially the dihydroxy bile acids, compared to the low income group. The faecal bile acids were also more highly degraded. The faecal flora contained more bacteroides and fewer eubacteria. Very few of the clostridia able to dehydrogenate the steroid nucleus were isolated. An epidemiological study based on street blocks indicated that the high income group also have a higher incidence of cancer of the large bowel and of the breast. The results are discussed in terms of theories on the aetiology of large bowel cancer.", "contents": "Faecal steroids and bacteria and large bowel cancer in Hong Kong by socio-economic groups. In a study of three socio-economic groups in Hong Kong, the high income group had a high faecal concentration of bile acids, especially the dihydroxy bile acids, compared to the low income group. The faecal bile acids were also more highly degraded. The faecal flora contained more bacteroides and fewer eubacteria. Very few of the clostridia able to dehydrogenate the steroid nucleus were isolated. An epidemiological study based on street blocks indicated that the high income group also have a higher incidence of cancer of the large bowel and of the breast. The results are discussed in terms of theories on the aetiology of large bowel cancer.", "PMID": 962996}
{"id": "pubmed23n0032_5307", "title": "Predictions of mortality from mesothelial tumours in asbestos factory workers.", "content": "Using the accumulated data on deaths from mesothelial tumours among cohorts of male and female factory workers at a London asbestos textile factory, the mortality from this cause up to the year 2000 AD has been predicted. The limitations of the methods used are pointed out, but it is estimated that for men the mortality due to mesothelial tumours will be between 7% and 11% of the total mortality and somewhat higher for women. The highest number of deaths from mesothelial tumours will occur during the 1980s, thereafter the numbers will decline because of the decreasing size of the cohort resulting from general mortality.", "contents": "Predictions of mortality from mesothelial tumours in asbestos factory workers. Using the accumulated data on deaths from mesothelial tumours among cohorts of male and female factory workers at a London asbestos textile factory, the mortality from this cause up to the year 2000 AD has been predicted. The limitations of the methods used are pointed out, but it is estimated that for men the mortality due to mesothelial tumours will be between 7% and 11% of the total mortality and somewhat higher for women. The highest number of deaths from mesothelial tumours will occur during the 1980s, thereafter the numbers will decline because of the decreasing size of the cohort resulting from general mortality.", "PMID": 962998}
{"id": "pubmed23n0032_5308", "title": "Monitoring liver disorders in vinyl chloride monomer workers using greyscale ultrasonography.", "content": "Recognition that vinyl chloride could be hepatotoxic led to a survey of workers to determine whether changes had been induced by past exposure, and to evaluate standard liver function tests as monitors of early liver abnormalities. Standard liver function tests were found to be unsuitable for the detection of such abnormalities in the population at risk. Of 487 workers examined, 102 (20-9%) had abnormalities on initial testing but only two were finally shown to have portal hypertension; in both cases, thrombocytopaenia provided the first diagnostic evidence since liver function tests were normal. Furthermore, 40 (35-7%) of 112 control subjects had initial test abnormalities. A sample of 19 workers with various exposures to vinyl chloride monomer were examined blind by greyscale ultrasonography. Five with minimal or no exposure were confirmed as normal but 12 of the remainder had abnormalities. These consisted of an enlarged portal vein (seven instances), splenomegaly (eight), and changes in hepatic texture (seven). Five of these 12 cases had previously been considered normal. It was concluded that greyscale ultrasonography had many advantages over standard methods for screening workers exposed to hepatotoxic chemicals, and should be the subject of a large scale evaluation.", "contents": "Monitoring liver disorders in vinyl chloride monomer workers using greyscale ultrasonography. Recognition that vinyl chloride could be hepatotoxic led to a survey of workers to determine whether changes had been induced by past exposure, and to evaluate standard liver function tests as monitors of early liver abnormalities. Standard liver function tests were found to be unsuitable for the detection of such abnormalities in the population at risk. Of 487 workers examined, 102 (20-9%) had abnormalities on initial testing but only two were finally shown to have portal hypertension; in both cases, thrombocytopaenia provided the first diagnostic evidence since liver function tests were normal. Furthermore, 40 (35-7%) of 112 control subjects had initial test abnormalities. A sample of 19 workers with various exposures to vinyl chloride monomer were examined blind by greyscale ultrasonography. Five with minimal or no exposure were confirmed as normal but 12 of the remainder had abnormalities. These consisted of an enlarged portal vein (seven instances), splenomegaly (eight), and changes in hepatic texture (seven). Five of these 12 cases had previously been considered normal. It was concluded that greyscale ultrasonography had many advantages over standard methods for screening workers exposed to hepatotoxic chemicals, and should be the subject of a large scale evaluation.", "PMID": 962999}
{"id": "pubmed23n0032_5309", "title": "Loss of pulmonary elastic recoil in workers formerly exposed to proteolytic enzyme (alcalase) in the detergent industry.", "content": "Sixty-seven workers in the detergent industry whose exposure to proteolytic enzyme ceased in 1969 have been examined clinically and functionally. By comparison with 42 lightly and moderately exposed subjects, 13 heavily exposed subjects showed significant loss of pulmonary elastic recoil as evidenced by increased lung volumes and increased pulmonary compliance, but there were no differences in airways resistance or other parameters of lung function. No difference was found between the two groups in relation to symptoms on exposure, current exercise tolerance, skin reactivity to the proteolytic enzyme alcalase, trypsin inhibitor capacity, and other features. An increased clinical grade of breathlessness was associated with evidence of airways obstruction, but not of altered elastic recoil. Comparison of the data on lung mechanics with results obtained in 1970 suggests that partial recovery of pulmonary elastic recoil may have occurred in some cases. It is also suggested that diminished elastic recoil, in the absence of impairment of transfer factor at rest, may reflect altered physical properties of the lung fibre network without loss of effective surface area available for gas exchange.", "contents": "Loss of pulmonary elastic recoil in workers formerly exposed to proteolytic enzyme (alcalase) in the detergent industry. Sixty-seven workers in the detergent industry whose exposure to proteolytic enzyme ceased in 1969 have been examined clinically and functionally. By comparison with 42 lightly and moderately exposed subjects, 13 heavily exposed subjects showed significant loss of pulmonary elastic recoil as evidenced by increased lung volumes and increased pulmonary compliance, but there were no differences in airways resistance or other parameters of lung function. No difference was found between the two groups in relation to symptoms on exposure, current exercise tolerance, skin reactivity to the proteolytic enzyme alcalase, trypsin inhibitor capacity, and other features. An increased clinical grade of breathlessness was associated with evidence of airways obstruction, but not of altered elastic recoil. Comparison of the data on lung mechanics with results obtained in 1970 suggests that partial recovery of pulmonary elastic recoil may have occurred in some cases. It is also suggested that diminished elastic recoil, in the absence of impairment of transfer factor at rest, may reflect altered physical properties of the lung fibre network without loss of effective surface area available for gas exchange.", "PMID": 963000}
{"id": "pubmed23n0032_5310", "title": "Byssinosis: a follow-up study of cotton ginnery workers in the Sudan.", "content": "A follow-up study of lung function tests and dust measurements was undertaken in ginnery workers employed in five ginning factories. Respiratory symptoms and respiratory function tests (FEV1 and FVC) were first recorded in 1967 on a total of 382 workers (323 permanently employed ginnery workers, 35 seasonal farfara workers, and 24 fire brigade men as controls). In 1969 after a six-month break from ginning before the start of the season, a follow-up study of lung function tests was undertaken on 96% of the same workers (306 ginnery workers, 35 farfara, and 24 fire brigade men). Because of the lapse of two years a new adjustment for age and height was made so as to compare the FEV1 measured in the follow-up study. The fire brigade men showed an expected fall in FEV1 during the two-year period, whereas the ginnery workers showed a rise presumably because they had had no dust exposure during the previous six months. The differences between the degree of change in these groups were statistically significant. In 1967 only the fine dust (less than 7 mum) was measured, while in 1969 the concentration of fine and medium dust, that is, less fly was measured. The factories were divided into three groups according to dust concentration. Comparison between the three factory groups and farfara shows a positive association between the level of dust concentration less fly and the prevalence of cough and phlegm. Since age did not appear to be a significant factor in the prevalence of byssinosis, comparisons between permanent workers in these three groups of factories and farfara workers taken separately were made without age standardization. The overall differences were statistically significant. There was a marked trend showing a positive association between prevalence of byssinosis and level of cotton dust concentration in the factories. The correlation between dust levels and the prevalence of byssinosis was nearly perfect when the time factor was included.", "contents": "Byssinosis: a follow-up study of cotton ginnery workers in the Sudan. A follow-up study of lung function tests and dust measurements was undertaken in ginnery workers employed in five ginning factories. Respiratory symptoms and respiratory function tests (FEV1 and FVC) were first recorded in 1967 on a total of 382 workers (323 permanently employed ginnery workers, 35 seasonal farfara workers, and 24 fire brigade men as controls). In 1969 after a six-month break from ginning before the start of the season, a follow-up study of lung function tests was undertaken on 96% of the same workers (306 ginnery workers, 35 farfara, and 24 fire brigade men). Because of the lapse of two years a new adjustment for age and height was made so as to compare the FEV1 measured in the follow-up study. The fire brigade men showed an expected fall in FEV1 during the two-year period, whereas the ginnery workers showed a rise presumably because they had had no dust exposure during the previous six months. The differences between the degree of change in these groups were statistically significant. In 1967 only the fine dust (less than 7 mum) was measured, while in 1969 the concentration of fine and medium dust, that is, less fly was measured. The factories were divided into three groups according to dust concentration. Comparison between the three factory groups and farfara shows a positive association between the level of dust concentration less fly and the prevalence of cough and phlegm. Since age did not appear to be a significant factor in the prevalence of byssinosis, comparisons between permanent workers in these three groups of factories and farfara workers taken separately were made without age standardization. The overall differences were statistically significant. There was a marked trend showing a positive association between prevalence of byssinosis and level of cotton dust concentration in the factories. The correlation between dust levels and the prevalence of byssinosis was nearly perfect when the time factor was included.", "PMID": 963001}
{"id": "pubmed23n0032_5311", "title": "Acute inhalation toxicity of cotton plant dusts.", "content": "The number of free lung-cells was studied in guinea-pigs after acute exposure to extracts of various cotton dusts. A good correlation was found between the increase in number of leucocytes in the airways and the number of Gram-negative bacteria in the different dusts. Experiments using the Shwartzmann reaction and the Limulus titration test demonstrated a relationship between the content of different endotoxins in the dusts and the pulmonary reaction. A model for the acute exposure effects after exposure to cotton dust is proposed.", "contents": "Acute inhalation toxicity of cotton plant dusts. The number of free lung-cells was studied in guinea-pigs after acute exposure to extracts of various cotton dusts. A good correlation was found between the increase in number of leucocytes in the airways and the number of Gram-negative bacteria in the different dusts. Experiments using the Shwartzmann reaction and the Limulus titration test demonstrated a relationship between the content of different endotoxins in the dusts and the pulmonary reaction. A model for the acute exposure effects after exposure to cotton dust is proposed.", "PMID": 963002}
{"id": "pubmed23n0032_5312", "title": "Energy expenditure and physiological performance of Sudanese cane cutters.", "content": "The thermal and exercise tolerances of 165 Sudanese cane cutters were measured in the laboratory and related to work performance and productivity in the cane fields. The results showed that the amount of cane cut per minute in the field was significantly correlated with changes in body weight (r = +0-53) during the third hour of work, aerobic energy expenditure (r = + 0-43), and cardiac frequency during work. These variables in turn were associated with predicted maximal power output (VO2 max) measured in the laboratory. The average energy expenditure during cane cutting was 1-66 +/- 0-33 1/min-1 (34-9 kJ/min-1) which represents approximately 60% of the workers predicted VO2 max. This rate of energy expenditure was sustained in the cane fields for at least three hours without significant pauses for rest. The sweat losses measured in 32 cane cutters during the two and three hours of work averaged 637 +/- 221 and 770 +/- 282 g/h-1 respectively, while the mean urine temperature immediately on cessation of effort was 37-74 +/- 0-46 degrees C. Despite the additional environmental heat load of the tropics, it would seem that cane cutters performing a self-paced task demanding heavy physical effort, are able to sustain work levels well in excess of those recommended for most European factory workers without obvious signs of fatigue or heat stress.", "contents": "Energy expenditure and physiological performance of Sudanese cane cutters. The thermal and exercise tolerances of 165 Sudanese cane cutters were measured in the laboratory and related to work performance and productivity in the cane fields. The results showed that the amount of cane cut per minute in the field was significantly correlated with changes in body weight (r = +0-53) during the third hour of work, aerobic energy expenditure (r = + 0-43), and cardiac frequency during work. These variables in turn were associated with predicted maximal power output (VO2 max) measured in the laboratory. The average energy expenditure during cane cutting was 1-66 +/- 0-33 1/min-1 (34-9 kJ/min-1) which represents approximately 60% of the workers predicted VO2 max. This rate of energy expenditure was sustained in the cane fields for at least three hours without significant pauses for rest. The sweat losses measured in 32 cane cutters during the two and three hours of work averaged 637 +/- 221 and 770 +/- 282 g/h-1 respectively, while the mean urine temperature immediately on cessation of effort was 37-74 +/- 0-46 degrees C. Despite the additional environmental heat load of the tropics, it would seem that cane cutters performing a self-paced task demanding heavy physical effort, are able to sustain work levels well in excess of those recommended for most European factory workers without obvious signs of fatigue or heat stress.", "PMID": 963003}
{"id": "pubmed23n0032_5313", "title": "Urinary non-precipitable lead in lead workers.", "content": "Sixty-six workers engaged in lead-glazing pottery with a presumed moderate exposure to lead were studied. The group comprised 20 men with long-term exposure to lead and positive laboratory signs of increased lead absorption (Group A); 22 with long-term exposure and negative laboratory signs (Group B); 11 with short-term exposure and positive laboratory signs (Group C); and 13 with short-term exposure and negative laboratory signs (Group D). In addition, 14 workers employed in casting the kelmet alloys with presumed heavy exposure to lead (Group E) and seven healthy individuals (Group F) were included. Urine samples from all the subjects were analysed to determine, first, the total lead using the ashing technique, and then the precipitable lead using the coprecipitation technique of Cholak, Hubbard, and Burkey (1948), but modified slightly by us. Thus, the non-precipitable lead fraction in urine was the difference between the two measurements and this was also expressed as a percentage of the total lead. The mean total lead and the mean proportion of non-precipitable lead were 0.62 mumol/l and 48.7%, 0.35 mumol/l and 44.9%, 0.40 mumol/l and 48.9%, 0.17 mumol/l and 24.6%, 1.43 mumol/l and 44.3%, 0.14 mumol/l and 18.8% for Groups A, B, C, D, E, and F respectively, showing that a large part of urinary lead was eliminated as precipitable lead in Groups D and F who had normal lead excretion, while about half was eliminated as non-precipitable lead in the other four groups who had excessive lead excretion. No essential difference in the proportion of non-precipitable lead among Groups A, B and C excluded the possibility that the proportion might be directly related to the period of exposure to lead and to the laboratory findings of excessive lead absorption. The mean proportion of non-precipitable lead for the physiological (up to 0.240 mumol/l), intermediate (0.241 to 0.721 mumol/l), and excessive (above 0.722 mumol/l) total lead levels was 26.7, 41.3, and 52.3% respectively, in the lead workers comprising Groups A, B, C, and E each showing increased lead excretion when grouped together. these data suggested that, when urinary lead is within the normal range, it is excreted largely as precipitable lead even in individuals exposed to lead, and that the principal conditions determining the excretion of non-precipitable lead would be the current or recent degree of lead absorption. The excretory mechanisms and the biological significance of the non-precipitable lead are also discussed.", "contents": "Urinary non-precipitable lead in lead workers. Sixty-six workers engaged in lead-glazing pottery with a presumed moderate exposure to lead were studied. The group comprised 20 men with long-term exposure to lead and positive laboratory signs of increased lead absorption (Group A); 22 with long-term exposure and negative laboratory signs (Group B); 11 with short-term exposure and positive laboratory signs (Group C); and 13 with short-term exposure and negative laboratory signs (Group D). In addition, 14 workers employed in casting the kelmet alloys with presumed heavy exposure to lead (Group E) and seven healthy individuals (Group F) were included. Urine samples from all the subjects were analysed to determine, first, the total lead using the ashing technique, and then the precipitable lead using the coprecipitation technique of Cholak, Hubbard, and Burkey (1948), but modified slightly by us. Thus, the non-precipitable lead fraction in urine was the difference between the two measurements and this was also expressed as a percentage of the total lead. The mean total lead and the mean proportion of non-precipitable lead were 0.62 mumol/l and 48.7%, 0.35 mumol/l and 44.9%, 0.40 mumol/l and 48.9%, 0.17 mumol/l and 24.6%, 1.43 mumol/l and 44.3%, 0.14 mumol/l and 18.8% for Groups A, B, C, D, E, and F respectively, showing that a large part of urinary lead was eliminated as precipitable lead in Groups D and F who had normal lead excretion, while about half was eliminated as non-precipitable lead in the other four groups who had excessive lead excretion. No essential difference in the proportion of non-precipitable lead among Groups A, B and C excluded the possibility that the proportion might be directly related to the period of exposure to lead and to the laboratory findings of excessive lead absorption. The mean proportion of non-precipitable lead for the physiological (up to 0.240 mumol/l), intermediate (0.241 to 0.721 mumol/l), and excessive (above 0.722 mumol/l) total lead levels was 26.7, 41.3, and 52.3% respectively, in the lead workers comprising Groups A, B, C, and E each showing increased lead excretion when grouped together. these data suggested that, when urinary lead is within the normal range, it is excreted largely as precipitable lead even in individuals exposed to lead, and that the principal conditions determining the excretion of non-precipitable lead would be the current or recent degree of lead absorption. The excretory mechanisms and the biological significance of the non-precipitable lead are also discussed.", "PMID": 963004}
{"id": "pubmed23n0032_5314", "title": "Erythrocyte fluorescence and lead intoxication.", "content": "Blood samples from people exposed to inorganic lead were examined by fluorescence microscopy for excess erythrocyte porphyrin. With continued lead absorption, fluorescent erythrocytes appeared in the circulation of workers handling this metal or its compounds, and they progressively increased in number and brilliance. These changes ensued if the blood lead concentration was maintained above 2-42 mumol/l (50 mug/100 ml), and preceded any material fall in the haemoglobin value. At one factory, 62-5% of 81 symptomless workers showed erythrocyte fluorescence attributable to the toxic effects of lead. Excess fluorocytes were found in blood samples from a child with pica and three of her eight siblings. These four were subsequently shown to have slightly increased blood lead concentrations (2-03 to 2-32 mumol/l). Fluorescence microscopy for excess erythrocyte porphyrin is a sensitive method for the detection of chronic lead intoxication. A relatively slight increase in the blood lead is associated with demonstrabel changes in erythrocyte porphyrin content. The procedure requires little blood, and may be performed upon stored samples collected for lead estimation. The results are not readily influenced by contamination, and provide good confirmatory evidence for the absorption of biochemically active lead.", "contents": "Erythrocyte fluorescence and lead intoxication. Blood samples from people exposed to inorganic lead were examined by fluorescence microscopy for excess erythrocyte porphyrin. With continued lead absorption, fluorescent erythrocytes appeared in the circulation of workers handling this metal or its compounds, and they progressively increased in number and brilliance. These changes ensued if the blood lead concentration was maintained above 2-42 mumol/l (50 mug/100 ml), and preceded any material fall in the haemoglobin value. At one factory, 62-5% of 81 symptomless workers showed erythrocyte fluorescence attributable to the toxic effects of lead. Excess fluorocytes were found in blood samples from a child with pica and three of her eight siblings. These four were subsequently shown to have slightly increased blood lead concentrations (2-03 to 2-32 mumol/l). Fluorescence microscopy for excess erythrocyte porphyrin is a sensitive method for the detection of chronic lead intoxication. A relatively slight increase in the blood lead is associated with demonstrabel changes in erythrocyte porphyrin content. The procedure requires little blood, and may be performed upon stored samples collected for lead estimation. The results are not readily influenced by contamination, and provide good confirmatory evidence for the absorption of biochemically active lead.", "PMID": 963005}
{"id": "pubmed23n0032_5315", "title": "Catalytic activation of transfer ribonucleic acid by a mammalian protein.", "content": "A tRNA activator has been isolated from mammalian organs which increases the capability of tRNA to accept certain amino acids through the action of mammalian aminoacyl-tRNA synthetases. This activity may be separated from the aminoacyl-tRNA synthetases for isoleucine, lysine, serine, and methionine by fractionation of liver or pancreas cytosol with ammonium sulfate or by chromatography over Sephadex G-200. The tRNA activating material is nondialyzable and is destroyed by trypsin or short heating. It acts catalytically. A molecular weight of approximately 45,000 was obtained by chromatography of tRNA activator on a calibrated Sephadex G-150 column. Activator increases acceptance of yeast tRNA for the amino acids isoleucine, leucine, lysine, serine, and methionine. It shows higher activity on liver tRNAMet f, tRNAMet m, and tRNALys than on unfractionated liver tRNA. Removal of protein from mammalian tRNA by extra phenol extractions, chromatography, or proteinase treatment increases its response to activator.", "contents": "Catalytic activation of transfer ribonucleic acid by a mammalian protein. A tRNA activator has been isolated from mammalian organs which increases the capability of tRNA to accept certain amino acids through the action of mammalian aminoacyl-tRNA synthetases. This activity may be separated from the aminoacyl-tRNA synthetases for isoleucine, lysine, serine, and methionine by fractionation of liver or pancreas cytosol with ammonium sulfate or by chromatography over Sephadex G-200. The tRNA activating material is nondialyzable and is destroyed by trypsin or short heating. It acts catalytically. A molecular weight of approximately 45,000 was obtained by chromatography of tRNA activator on a calibrated Sephadex G-150 column. Activator increases acceptance of yeast tRNA for the amino acids isoleucine, leucine, lysine, serine, and methionine. It shows higher activity on liver tRNAMet f, tRNAMet m, and tRNALys than on unfractionated liver tRNA. Removal of protein from mammalian tRNA by extra phenol extractions, chromatography, or proteinase treatment increases its response to activator.", "PMID": 963009}
{"id": "pubmed23n0032_5316", "title": "Nearest neighbor analysis of heparin: identification and quantitation of the products formed by selective depolymerization procedures.", "content": "Heparin was carboxyl-reduced with sodium boro[3H]hydride and converted to a mixture of oligosaccharides by treatment with nitrous acid at pH 2. The oligosaccharide mixture was aldehyde-reduced with sodium boro[3H]hydride and the mixture of products, labeled both in the hexoses formed in the carboxyl-reduction step and in the reducing sugars formed in the nitrous acid reaction, was separated and analyzed. The major product, L-idosyl 2-sulfate leads to anhydro-D-mannitol 6-sulfate (I), contained 60% of hexoses derived from the hexuronic acid residues in the original heparin. A second product, which contained 15% of the hexoses derived from the hexuronic acid residues in the original heparin, was identified as a tetrasaccharide composed of two L-idosyl 2-sulfate residues, one anhydro-D-mannitol 6-sulfate residue (the reducing end),and a hydroxymethylpentose sulfate residue formed by deamination of a disulfated D-glucosamine residue without bond cleavage. Several additional disaccharides derived from the regions of the polymer which contained D-glucuronic acid residues and lower degrees of O-sulfation were also identified among the deamination products. The oligosaccharides that were obtained accounted for 100% of the original carboxyl-reduced heparin, and paper chromatographic profiles of the oligosaccharide separations can be used as a fingerprint of the heparin preparation. The properties of I were examined in greater detail. The glycosidic bond of the L-idosyl 2-sulfate residue was found to be extremely labile to 0.1 N HCl at 100 degrees C, hydrolyzing with a t 1/2 of 18 min to give high yields of L-idose 2-sulfate and anhydro-D-mannitol 6-sulfate. L-Idofuranose was also identified as an intermediate in the conversion of L-idose 2-sulfate to L-idosan. The acid lability of the L-idosyl 2-sulfate bond in I offers a new route for the selective cleavage of carboxyl-reduced heparin.", "contents": "Nearest neighbor analysis of heparin: identification and quantitation of the products formed by selective depolymerization procedures. Heparin was carboxyl-reduced with sodium boro[3H]hydride and converted to a mixture of oligosaccharides by treatment with nitrous acid at pH 2. The oligosaccharide mixture was aldehyde-reduced with sodium boro[3H]hydride and the mixture of products, labeled both in the hexoses formed in the carboxyl-reduction step and in the reducing sugars formed in the nitrous acid reaction, was separated and analyzed. The major product, L-idosyl 2-sulfate leads to anhydro-D-mannitol 6-sulfate (I), contained 60% of hexoses derived from the hexuronic acid residues in the original heparin. A second product, which contained 15% of the hexoses derived from the hexuronic acid residues in the original heparin, was identified as a tetrasaccharide composed of two L-idosyl 2-sulfate residues, one anhydro-D-mannitol 6-sulfate residue (the reducing end),and a hydroxymethylpentose sulfate residue formed by deamination of a disulfated D-glucosamine residue without bond cleavage. Several additional disaccharides derived from the regions of the polymer which contained D-glucuronic acid residues and lower degrees of O-sulfation were also identified among the deamination products. The oligosaccharides that were obtained accounted for 100% of the original carboxyl-reduced heparin, and paper chromatographic profiles of the oligosaccharide separations can be used as a fingerprint of the heparin preparation. The properties of I were examined in greater detail. The glycosidic bond of the L-idosyl 2-sulfate residue was found to be extremely labile to 0.1 N HCl at 100 degrees C, hydrolyzing with a t 1/2 of 18 min to give high yields of L-idose 2-sulfate and anhydro-D-mannitol 6-sulfate. L-Idofuranose was also identified as an intermediate in the conversion of L-idose 2-sulfate to L-idosan. The acid lability of the L-idosyl 2-sulfate bond in I offers a new route for the selective cleavage of carboxyl-reduced heparin.", "PMID": 963010}
{"id": "pubmed23n0032_5317", "title": "2-Imino-2-methoxyethyl 1-thioglycosides: new reagents for attaching sugars to proteins.", "content": "Cyanomethyl 1-thioglycosides ofD-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and D-mannose were prepared from the respective pseudothiourea derivatives and chloroacetonitrile. The nitrile group in these cyanomethyl thioglycosides can be converted to a methyl imidate group by treatment with sodium methoxide or HC1 in dry methanol to yield 2-imino-2-methoxyethyl 1-thioglycosides (IME-thioglycosides). The factors influencing the yield of IME-thioglycosides were investigated. The most convenient method of preparing IME-thioglycosides was treating 0.1 M cyanomethyl thioglycoside peracetate in dry methanol with 0.01 M sodium methoxide at room temperature for 24-48 h (50-60% yield). These IME-thioglycosides reacted readily with simple amines, amino acids, and proteins in mildly alkaline buffer solutions. Alpha-amylase and lysozyme modified with these reagents under appropriate conditions retained full activities. Thus the IME-thioglycosides constitute a new group of reagents for attaching sugars to proteins.", "contents": "2-Imino-2-methoxyethyl 1-thioglycosides: new reagents for attaching sugars to proteins. Cyanomethyl 1-thioglycosides ofD-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and D-mannose were prepared from the respective pseudothiourea derivatives and chloroacetonitrile. The nitrile group in these cyanomethyl thioglycosides can be converted to a methyl imidate group by treatment with sodium methoxide or HC1 in dry methanol to yield 2-imino-2-methoxyethyl 1-thioglycosides (IME-thioglycosides). The factors influencing the yield of IME-thioglycosides were investigated. The most convenient method of preparing IME-thioglycosides was treating 0.1 M cyanomethyl thioglycoside peracetate in dry methanol with 0.01 M sodium methoxide at room temperature for 24-48 h (50-60% yield). These IME-thioglycosides reacted readily with simple amines, amino acids, and proteins in mildly alkaline buffer solutions. Alpha-amylase and lysozyme modified with these reagents under appropriate conditions retained full activities. Thus the IME-thioglycosides constitute a new group of reagents for attaching sugars to proteins.", "PMID": 963012}
{"id": "pubmed23n0032_5318", "title": "Attachment of thioglycosides to proteins: enhancement of liver membrane binding.", "content": "Thioglycosides of D-galactose, D-glucose, N-acetyl-D-glucosamine, and D-mannose were covalently attached to Aspergillus oryzae alpha-amylase, hen's eggs lysozyme, and bovine serum albumin by amidination, diazo coupling, and amide formation. The binding of the newly formed glycoproteins (neoglycoproteins) to rabbit liver membranes was measured, using asialoorosomucoid as a reference. Attachment of D-galactosides by any of the three methods enhanced binding by several orders of magnitude. Coupling of a comparable number of D-mannosides or N-acetyl-D-glucosaminides had little or no effect. Attachment of D-glucosides also enhanced binding but to a variable extent depending on the method of attachment. Thus, the behavior of neoglycoproteins toward rabbit liver membranes closely paralleled that of serum glycoproteins (Ashwell and Morell, 1974) with respect to sugar specificity.", "contents": "Attachment of thioglycosides to proteins: enhancement of liver membrane binding. Thioglycosides of D-galactose, D-glucose, N-acetyl-D-glucosamine, and D-mannose were covalently attached to Aspergillus oryzae alpha-amylase, hen's eggs lysozyme, and bovine serum albumin by amidination, diazo coupling, and amide formation. The binding of the newly formed glycoproteins (neoglycoproteins) to rabbit liver membranes was measured, using asialoorosomucoid as a reference. Attachment of D-galactosides by any of the three methods enhanced binding by several orders of magnitude. Coupling of a comparable number of D-mannosides or N-acetyl-D-glucosaminides had little or no effect. Attachment of D-glucosides also enhanced binding but to a variable extent depending on the method of attachment. Thus, the behavior of neoglycoproteins toward rabbit liver membranes closely paralleled that of serum glycoproteins (Ashwell and Morell, 1974) with respect to sugar specificity.", "PMID": 963013}
{"id": "pubmed23n0032_5319", "title": "Biosynthesis of steroid sulfates by the boar testes.", "content": "The steroidogenic enzymes present in boar testicular tissue have been shown to use steroid sulfates as substrates. Pregnenolone sulfate, doubly labeled with 3H in the nucleus and 35S, was incubated with the microsomal fraction of boar testicular tissue and a reduced triphosphopyridine nucleotide generating system and 17-hydroxypregnenolone sulfate as well as dehydroisoandrosterone sulfate were isolated. These products had the same 3H to 35S ratio as the substrate demonstrating that the conversions had taken place with the sulfate group intact. Thus testicular 17-hydroxylase and C-17,20-desmolase can convert delta5-3beta-yl sulfates into products which still contain the sulfate group at C-3. An unusual steroid sulfate, doubly labeled 5,16-androstadien-3beta-yl sulfate, was also isolated demonstrating that such an olefin can be biosynthetized via steroid sulfate pathways. In a second experiment small amounts of [3H]-21-hydroxypregnenolone sulfate were isolated from the incubation of [3H]pregnenolone sulfate with microsomes from boar testes. The isolation of the 21-hydroxylated steroid sulfate is taken as support for the hypothesis that delta16 steroids are biosynthesized from their C21 precursors by events that are initiated by oxygenation at C-21.", "contents": "Biosynthesis of steroid sulfates by the boar testes. The steroidogenic enzymes present in boar testicular tissue have been shown to use steroid sulfates as substrates. Pregnenolone sulfate, doubly labeled with 3H in the nucleus and 35S, was incubated with the microsomal fraction of boar testicular tissue and a reduced triphosphopyridine nucleotide generating system and 17-hydroxypregnenolone sulfate as well as dehydroisoandrosterone sulfate were isolated. These products had the same 3H to 35S ratio as the substrate demonstrating that the conversions had taken place with the sulfate group intact. Thus testicular 17-hydroxylase and C-17,20-desmolase can convert delta5-3beta-yl sulfates into products which still contain the sulfate group at C-3. An unusual steroid sulfate, doubly labeled 5,16-androstadien-3beta-yl sulfate, was also isolated demonstrating that such an olefin can be biosynthetized via steroid sulfate pathways. In a second experiment small amounts of [3H]-21-hydroxypregnenolone sulfate were isolated from the incubation of [3H]pregnenolone sulfate with microsomes from boar testes. The isolation of the 21-hydroxylated steroid sulfate is taken as support for the hypothesis that delta16 steroids are biosynthesized from their C21 precursors by events that are initiated by oxygenation at C-21.", "PMID": 963014}
{"id": "pubmed23n0032_5320", "title": "Role of cyclic electron transport in photosynthesis as measured by the photoinduced turnover of P700 in vivo.", "content": "The light-induced turnover of P700 was measured spectrophotometrically in a wide variety of algae and some photosynthetic mutants. Analysis of the postillumination recovery of P700+ revealed that the apparent first-order rate constant for reduction via the cyclic pathway was much lower that that via the noncyclic pathway. After activation of photosystems 1 and 2 the half-time for reduction of P700+ was 5-20 ms, whereas after activation of primarily photosystem 1 a longer half-time of ca. 150 ms was observed. The extent of the photooxidation of P700 was the same in both regimes of illumination. The longer half-time was also noted after inhibition of photosystem 2 by 3-(3,4-dichlorophenyl)-1,1-dimethylurea or mild heat shock and in mutant algae known to lack a functional photosystem 2. No signal was observed in mutants lacking P700 itself but those strains lacking either plastocyanin or cytochrome f were capable of a very slow turnover (reduction t 1/2 greater than 500 ms at room temperature). This very slow turnover was not affected by carbonyl cyanide m-chlorophenylhydrazone or the plastoquinone antagonist, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, indicating that the pathway for reduction of P700+ in these mutants is not energy linked and does not utilize the intersystem electron transport chain. The slow, 150 ms, reduction of P700+ due to cyclic flow was not observed when cells were engaged in photosynthesis at high-light intensities. The data are interpreted as evidence for the involvement of the total functional pool of P700 in both electron transport pathways, and we suggest that cyclic electron transport does not contribute to photosynthesis in oxygen-evolving autotrophs.", "contents": "Role of cyclic electron transport in photosynthesis as measured by the photoinduced turnover of P700 in vivo. The light-induced turnover of P700 was measured spectrophotometrically in a wide variety of algae and some photosynthetic mutants. Analysis of the postillumination recovery of P700+ revealed that the apparent first-order rate constant for reduction via the cyclic pathway was much lower that that via the noncyclic pathway. After activation of photosystems 1 and 2 the half-time for reduction of P700+ was 5-20 ms, whereas after activation of primarily photosystem 1 a longer half-time of ca. 150 ms was observed. The extent of the photooxidation of P700 was the same in both regimes of illumination. The longer half-time was also noted after inhibition of photosystem 2 by 3-(3,4-dichlorophenyl)-1,1-dimethylurea or mild heat shock and in mutant algae known to lack a functional photosystem 2. No signal was observed in mutants lacking P700 itself but those strains lacking either plastocyanin or cytochrome f were capable of a very slow turnover (reduction t 1/2 greater than 500 ms at room temperature). This very slow turnover was not affected by carbonyl cyanide m-chlorophenylhydrazone or the plastoquinone antagonist, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, indicating that the pathway for reduction of P700+ in these mutants is not energy linked and does not utilize the intersystem electron transport chain. The slow, 150 ms, reduction of P700+ due to cyclic flow was not observed when cells were engaged in photosynthesis at high-light intensities. The data are interpreted as evidence for the involvement of the total functional pool of P700 in both electron transport pathways, and we suggest that cyclic electron transport does not contribute to photosynthesis in oxygen-evolving autotrophs.", "PMID": 963015}
{"id": "pubmed23n0032_5321", "title": "Proton magnetic resonance study of the intramolecular association and conformation of the alpha and beta pyridine mononucleotides and nucleosides.", "content": "The chemical shifts and coupling constants are reported for the proton nuclear magnetic resonance (NMR) spectra of the alpha and beta anomers of the oxidized and reduced pyridine mononucleotides and nucleosides. The pseudorotational conformational analyses of the ribose coupling constants indicate that the ribose conformation for beta-nicotinamide mononucleotide, beta NMN, can best be described by a 3:1 mixture of interconverting 3'-exo (S) and 2'-exo (N) conformers. Reduction of betaNMN to betaNMNH results in phase angles consistent with interconverting 2'-endo (S) and 3'-endo (N) conformers without changes in the conformer populations. Cleavage of the 5'-phosphate from betaNMN has a significant effect on the phase angles (becoming more like those for betaNMNH), conformer population (the N and S conformers become nearly equal), and the distribution of the rotational isomers around the ribose 4'-5' bond to the exocyclic methylene (the gauche-gauche population decreases by about 25%). In contrast, for betaNMNH these parameters are all insensitive to dephosphorylation. The pseudorotational analysis has been extended to define the conformational parameters of alpha nucleotides. Analysis of the coupling constants for the alpha anomers indicates that the phase angles, conformer populations, and rotational isomers are generally insensitive to dephosphorylation, whereas both the phase angle and conformer populations are strongly dependent on the redox state of the base, alphaNMN being predominantly 2'-endo and alphaNMNH exclusively 2'-exo. The rotational isomers around the 4'-5' and 5'- O bonds are found to be insensitive to.the large changes in ribose conformation in the absence of any interaction with the base. The results are discussed in terms of relative contributions from base-ribose, ribose-side chain, and base-side chain interactions to the general conformational restraints imposed by the cis-2'-3'-hydroxyl interaction in beta nucleotides and the additional cis-2'-hydroxyl-base interaction in alpha nucleotides. The significance of these interactions with respect to the enzymatic and nonenzymatic properties of the pyridine nucleotides is also considered.", "contents": "Proton magnetic resonance study of the intramolecular association and conformation of the alpha and beta pyridine mononucleotides and nucleosides. The chemical shifts and coupling constants are reported for the proton nuclear magnetic resonance (NMR) spectra of the alpha and beta anomers of the oxidized and reduced pyridine mononucleotides and nucleosides. The pseudorotational conformational analyses of the ribose coupling constants indicate that the ribose conformation for beta-nicotinamide mononucleotide, beta NMN, can best be described by a 3:1 mixture of interconverting 3'-exo (S) and 2'-exo (N) conformers. Reduction of betaNMN to betaNMNH results in phase angles consistent with interconverting 2'-endo (S) and 3'-endo (N) conformers without changes in the conformer populations. Cleavage of the 5'-phosphate from betaNMN has a significant effect on the phase angles (becoming more like those for betaNMNH), conformer population (the N and S conformers become nearly equal), and the distribution of the rotational isomers around the ribose 4'-5' bond to the exocyclic methylene (the gauche-gauche population decreases by about 25%). In contrast, for betaNMNH these parameters are all insensitive to dephosphorylation. The pseudorotational analysis has been extended to define the conformational parameters of alpha nucleotides. Analysis of the coupling constants for the alpha anomers indicates that the phase angles, conformer populations, and rotational isomers are generally insensitive to dephosphorylation, whereas both the phase angle and conformer populations are strongly dependent on the redox state of the base, alphaNMN being predominantly 2'-endo and alphaNMNH exclusively 2'-exo. The rotational isomers around the 4'-5' and 5'- O bonds are found to be insensitive to.the large changes in ribose conformation in the absence of any interaction with the base. The results are discussed in terms of relative contributions from base-ribose, ribose-side chain, and base-side chain interactions to the general conformational restraints imposed by the cis-2'-3'-hydroxyl interaction in beta nucleotides and the additional cis-2'-hydroxyl-base interaction in alpha nucleotides. The significance of these interactions with respect to the enzymatic and nonenzymatic properties of the pyridine nucleotides is also considered.", "PMID": 963016}
{"id": "pubmed23n0032_5322", "title": "The construction and testing of a simple, slow delivery-rapid quench apparatus.", "content": "A simple, inexpensive, slow delivery-rapid quench apparatus is described. The apparatus can be used to mix small volumes (about 50 mul) of equilibrium mixtures of enzyme-substrate and enzyme-product complexes with a quenching solution, ideally to inactivate the enzyme more rapidly than such complexes can be inconverted. The efficiency of the apparatus is tested by (a) injecting basic solutions of an indicator dye into acid and observing the length of the unbleached plume of dye produced at the delivery tip and (b) by forming an enzyme-substrate complex with 32P-labeled phosphoglucomutase and measuring the extent of label transfer prior to inactivation by the quenching solution. Problems that may be encountered in attempts to inactivate equilibrium mixtures of enzyme-substrate and enzyme-product complexes without producing quenching artifacts are considered.", "contents": "The construction and testing of a simple, slow delivery-rapid quench apparatus. A simple, inexpensive, slow delivery-rapid quench apparatus is described. The apparatus can be used to mix small volumes (about 50 mul) of equilibrium mixtures of enzyme-substrate and enzyme-product complexes with a quenching solution, ideally to inactivate the enzyme more rapidly than such complexes can be inconverted. The efficiency of the apparatus is tested by (a) injecting basic solutions of an indicator dye into acid and observing the length of the unbleached plume of dye produced at the delivery tip and (b) by forming an enzyme-substrate complex with 32P-labeled phosphoglucomutase and measuring the extent of label transfer prior to inactivation by the quenching solution. Problems that may be encountered in attempts to inactivate equilibrium mixtures of enzyme-substrate and enzyme-product complexes without producing quenching artifacts are considered.", "PMID": 963017}
{"id": "pubmed23n0032_5323", "title": "Thermodynamics and mechanism of the PO3 transfer process in the phosphoglucomutase reaction.", "content": "The equilibria among the central complexes in the phosphoglucomutase system were evaluated by (a) using an excess of enzyme plus Mg2+ to prepare mixtures with glucose phosphates in which essentially no free glucose phosphates were present; (b) inactivating the enzyme in such mixtures by means of a procedure that prevents substantial interconversion of the central complexes; and (c) assaying the quenched mixture for glucose 1-P, glucose 1-6-P2, and glucose-6-P. The fractional amounts of Ep-Mg-Glc-1-P, ED-Mg-Glc-P2, and Ep-Mg-Glc-6-P present at pH 7.5 and 24 degrees C were 0.13, 0.54, and 0.33. (Ep and ED are the phospho and dephospho forms of the enzyme, respectively). From these fractions and the equilibrium isotope exchange constants for the three sugar phosphates, true dissociation constants can be calculated for each of the above complexes: 8.5 muM, 19 nM, and 57 muM, respectively. Relative to the rate of PO3 transfer to water, a 3 x 10(10)-fold rate increase is produced by binding glucose-1-P to the Mg2+-enzyme (Ray, jr., W.J., Long, J.W., and Owens, J.D. (1976), Biochemistry, the following paper in this issue). This \"substrate-induced rate effect\" is equivalent to a difference of some 14 kcal in Gibbs activation energies for transfer to chemically similar hydroxyl groups, and most of this energy difference ultimately must be rationalized in terms of binding interactions involving the phosphoglucosyl moiety. Three different mechanisms for using substrate binding energy to reduce the activation energy of the subsequent catalytic step are examined as possible explanations for the substrate-induced rate effect. These mechanisms emphasize (a) enthalpic destabilization and (b) (entropic) immobilization of reactant groups during formation of the enzyme-substrate complex, and (c) increased binding interactions of nonreactant groups during the subsequent approach to the transition state. As a test for enthalpic destabilization of the enzymic phosphate group, values of deltaG degrees' for the hydrolytic cleavage of this group in Ep and Ep-Glc-1-P are calculated from equilibria measured at pH 7.5 and 30 degrees C: about -1 and +1.4 kcal/mol, respectively. To test for destabilization of the acceptor hydroxyl group in the enzyme-substrate complex, deltaG degrees' for equilibrium, Ep-Glc-P in equilibrium ED-Glc-P2, is compared with that for the corresponding process involving the nonrigid acceptor, 1,4-butanediol monophosphate: about -0.9 and -1.9 kcal, respectively. These results are not consistent with a large enthalpic destabilization of the reactant groups in the Ep-Glc-1-P complex. To test for entropic immobilization of reactant groups, glucose-6-phosphate is considered as a bidentate ligand, and the chelate effect on the binding and subsequent enzymic transfer reaction that arises from covalently linked the sugar ring and the PO3 group is evaluated. Reference reactions involving xylose as a PO3 acceptor both in the presence and absence of bond (inorganic) phosphite are used...", "contents": "Thermodynamics and mechanism of the PO3 transfer process in the phosphoglucomutase reaction. The equilibria among the central complexes in the phosphoglucomutase system were evaluated by (a) using an excess of enzyme plus Mg2+ to prepare mixtures with glucose phosphates in which essentially no free glucose phosphates were present; (b) inactivating the enzyme in such mixtures by means of a procedure that prevents substantial interconversion of the central complexes; and (c) assaying the quenched mixture for glucose 1-P, glucose 1-6-P2, and glucose-6-P. The fractional amounts of Ep-Mg-Glc-1-P, ED-Mg-Glc-P2, and Ep-Mg-Glc-6-P present at pH 7.5 and 24 degrees C were 0.13, 0.54, and 0.33. (Ep and ED are the phospho and dephospho forms of the enzyme, respectively). From these fractions and the equilibrium isotope exchange constants for the three sugar phosphates, true dissociation constants can be calculated for each of the above complexes: 8.5 muM, 19 nM, and 57 muM, respectively. Relative to the rate of PO3 transfer to water, a 3 x 10(10)-fold rate increase is produced by binding glucose-1-P to the Mg2+-enzyme (Ray, jr., W.J., Long, J.W., and Owens, J.D. (1976), Biochemistry, the following paper in this issue). This \"substrate-induced rate effect\" is equivalent to a difference of some 14 kcal in Gibbs activation energies for transfer to chemically similar hydroxyl groups, and most of this energy difference ultimately must be rationalized in terms of binding interactions involving the phosphoglucosyl moiety. Three different mechanisms for using substrate binding energy to reduce the activation energy of the subsequent catalytic step are examined as possible explanations for the substrate-induced rate effect. These mechanisms emphasize (a) enthalpic destabilization and (b) (entropic) immobilization of reactant groups during formation of the enzyme-substrate complex, and (c) increased binding interactions of nonreactant groups during the subsequent approach to the transition state. As a test for enthalpic destabilization of the enzymic phosphate group, values of deltaG degrees' for the hydrolytic cleavage of this group in Ep and Ep-Glc-1-P are calculated from equilibria measured at pH 7.5 and 30 degrees C: about -1 and +1.4 kcal/mol, respectively. To test for destabilization of the acceptor hydroxyl group in the enzyme-substrate complex, deltaG degrees' for equilibrium, Ep-Glc-P in equilibrium ED-Glc-P2, is compared with that for the corresponding process involving the nonrigid acceptor, 1,4-butanediol monophosphate: about -0.9 and -1.9 kcal, respectively. These results are not consistent with a large enthalpic destabilization of the reactant groups in the Ep-Glc-1-P complex. To test for entropic immobilization of reactant groups, glucose-6-phosphate is considered as a bidentate ligand, and the chelate effect on the binding and subsequent enzymic transfer reaction that arises from covalently linked the sugar ring and the PO3 group is evaluated. Reference reactions involving xylose as a PO3 acceptor both in the presence and absence of bond (inorganic) phosphite are used...", "PMID": 963018}
{"id": "pubmed23n0032_5324", "title": "An analysis of the substrate-induced rate effect in the phosphoglucomutase system.", "content": "The rate constant for the catalytic transfer of the active-site PO3 group from rabbit muscle phosphoglucomutase to the hydroxyl group of a water molecule is about 3 x 10(-8) s-1 under optimal reaction conditions, but in the absence of the normal substrate, viz., at pH 7.5 and 30 degrees C, in the presence of saturating Mg2+; the corresponding constant for transfer to the 6-hydroxyl group of glucose 1-phosphate under analogous conditions, about 1000 s-1, is larger than this by some 3 x 10(10)-fold. Since no single factor appears to be capable of providing a rationale for a majority of this \"substrate-induced rate effect\" (Ray, jr., W.J., and Long, J.W. (1976), Biochemistry, the preceding paper in this issue), the change in the PO3-transfer rate produced by binding various parts of the phosphoglucosyl moiety to the enzyme, both separately and concurrently, was investigated. The rate of PO3 transfer to water is increased by up to 1000-fold by binding entities that provide the active site with a second PO3 group, e.g., ethyl phosphate or inorganic phosphite. Using an alcoholic acceptor further increases transfer efficiency (in the presence of bound phosphite): increase with methanol, about 2000-fold on a molar basis. The reactivities of ten other primary aliphatic alcohols vary by nearly 600-fold as the acidity of the PO3 acceptor is varied over a 4000-fold range. Although no straightforward relationship is observed between the efficiency of an alcohol as an acceptor and its acidity - presumably because of complications due to steric effects, for example - an increased transfer rate of 100-fold, relative to the water reaction, is estimated for a simple primary alcohol with a pKa similar to that expected for the 6-hydroxyl group of glucose 1-phosphate, when the alcohol is present at a concentration of 1 M. Joining an alcoholic acceptor and a PO3 group via five apparently inert bridging units changes PO3 transfer to an intramolecular process; in the case of 1,4-butanediol monophosphate the rate of transfer also increases by 240-fold, relative to the analogous reaction in the presence of 1 M propanol and bound inorganic phosphite. Comparable values also are obtained in comparisons of PO3 transfer rates for trans- 1,4-butenediol and 1,4-butynediol monophosphates relative to 1 M allyl and propargyl alcohols, respectively, in the presence of bound phosphite. An increased rate of transfer also is produced by binding the xylosyl part of the glucose ring, either when the acceptor is an hydroxyl group attached to the ring or when it is the hydroxyl group of a water molecule, e.g., as in the water reaction facilitated by bound xylose 1-phosphate. These and other results suggest that most of the differences between the rates of the water reaction and the glucose 1-phosphate reaction can be rationalized in terms of four fairly discrete factors whose approximate values are as follows: the PO4 factor, 1000-fold; the C-OH/H-OH factor, 100-fold; the nucleophile-binding factor, 250-fold; and the (CHOH)3-bridging factor, 200-fold...", "contents": "An analysis of the substrate-induced rate effect in the phosphoglucomutase system. The rate constant for the catalytic transfer of the active-site PO3 group from rabbit muscle phosphoglucomutase to the hydroxyl group of a water molecule is about 3 x 10(-8) s-1 under optimal reaction conditions, but in the absence of the normal substrate, viz., at pH 7.5 and 30 degrees C, in the presence of saturating Mg2+; the corresponding constant for transfer to the 6-hydroxyl group of glucose 1-phosphate under analogous conditions, about 1000 s-1, is larger than this by some 3 x 10(10)-fold. Since no single factor appears to be capable of providing a rationale for a majority of this \"substrate-induced rate effect\" (Ray, jr., W.J., and Long, J.W. (1976), Biochemistry, the preceding paper in this issue), the change in the PO3-transfer rate produced by binding various parts of the phosphoglucosyl moiety to the enzyme, both separately and concurrently, was investigated. The rate of PO3 transfer to water is increased by up to 1000-fold by binding entities that provide the active site with a second PO3 group, e.g., ethyl phosphate or inorganic phosphite. Using an alcoholic acceptor further increases transfer efficiency (in the presence of bound phosphite): increase with methanol, about 2000-fold on a molar basis. The reactivities of ten other primary aliphatic alcohols vary by nearly 600-fold as the acidity of the PO3 acceptor is varied over a 4000-fold range. Although no straightforward relationship is observed between the efficiency of an alcohol as an acceptor and its acidity - presumably because of complications due to steric effects, for example - an increased transfer rate of 100-fold, relative to the water reaction, is estimated for a simple primary alcohol with a pKa similar to that expected for the 6-hydroxyl group of glucose 1-phosphate, when the alcohol is present at a concentration of 1 M. Joining an alcoholic acceptor and a PO3 group via five apparently inert bridging units changes PO3 transfer to an intramolecular process; in the case of 1,4-butanediol monophosphate the rate of transfer also increases by 240-fold, relative to the analogous reaction in the presence of 1 M propanol and bound inorganic phosphite. Comparable values also are obtained in comparisons of PO3 transfer rates for trans- 1,4-butenediol and 1,4-butynediol monophosphates relative to 1 M allyl and propargyl alcohols, respectively, in the presence of bound phosphite. An increased rate of transfer also is produced by binding the xylosyl part of the glucose ring, either when the acceptor is an hydroxyl group attached to the ring or when it is the hydroxyl group of a water molecule, e.g., as in the water reaction facilitated by bound xylose 1-phosphate. These and other results suggest that most of the differences between the rates of the water reaction and the glucose 1-phosphate reaction can be rationalized in terms of four fairly discrete factors whose approximate values are as follows: the PO4 factor, 1000-fold; the C-OH/H-OH factor, 100-fold; the nucleophile-binding factor, 250-fold; and the (CHOH)3-bridging factor, 200-fold...", "PMID": 963019}
{"id": "pubmed23n0032_5325", "title": "The thermodynamic and structural differences among the catalytically active complexes of phosphoglucomutase: metal ion effects.", "content": "When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ED are the phospho and dephospho forms of the enzyme). This altered distribution is caused by a metal-specific change in the equilibrium constant for transfer of the enzymic PO3 group to bound glucose monophosphates: 65-fold as M is varied from Zn2+ to Cd2+. This change in equilibrium is related to metal-specific differences in chemical potential of the phosphate group in the Ep-M complex; these differences in chemical potential remain in the Ep-M-Glc-1-P and Ep-M-Glc-6-P complexes, but essentially disappear in the ED-M-Glc-1,6-P2 complex. If glucose monophosphates are considered as substrates, and glucose bisphosphate as the product, there is a direct relationship between the equilibrium concentration of enzyme-substrate and enzyme-product complexes (when these are varied by changing the identity of the bound metal ion) and the ultraviolet spectrum of the equilibrium mixture of complexes, as assessed by difference spectroscopy (Peck, E.J., Jr., and Ray, W.J., Jr. (1969), J. Biol, Chem. 244, 3754). These spectral changes apparently are caused by an alteration in the conformation of the enzyme during transfer of a PO3 group between the enzyme and the glucose phosphate moiety, or as the result of it. The extent to which conformational changes accompany group-transfer processes in other enzymic systems is not clear, but it is possible that analogous changes may help to account for the \"half-of-the-sites reactivity\" observed with a number of multimeric enzymes.", "contents": "The thermodynamic and structural differences among the catalytically active complexes of phosphoglucomutase: metal ion effects. When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ED are the phospho and dephospho forms of the enzyme). This altered distribution is caused by a metal-specific change in the equilibrium constant for transfer of the enzymic PO3 group to bound glucose monophosphates: 65-fold as M is varied from Zn2+ to Cd2+. This change in equilibrium is related to metal-specific differences in chemical potential of the phosphate group in the Ep-M complex; these differences in chemical potential remain in the Ep-M-Glc-1-P and Ep-M-Glc-6-P complexes, but essentially disappear in the ED-M-Glc-1,6-P2 complex. If glucose monophosphates are considered as substrates, and glucose bisphosphate as the product, there is a direct relationship between the equilibrium concentration of enzyme-substrate and enzyme-product complexes (when these are varied by changing the identity of the bound metal ion) and the ultraviolet spectrum of the equilibrium mixture of complexes, as assessed by difference spectroscopy (Peck, E.J., Jr., and Ray, W.J., Jr. (1969), J. Biol, Chem. 244, 3754). These spectral changes apparently are caused by an alteration in the conformation of the enzyme during transfer of a PO3 group between the enzyme and the glucose phosphate moiety, or as the result of it. The extent to which conformational changes accompany group-transfer processes in other enzymic systems is not clear, but it is possible that analogous changes may help to account for the \"half-of-the-sites reactivity\" observed with a number of multimeric enzymes.", "PMID": 963020}
{"id": "pubmed23n0032_5326", "title": "Synthesis of human [9-leucine-B] Insulin.", "content": "The synthesis and isolation in purified form of [Leu9-B]insulin, a biologically active analogue of human insulin, is described. This analogue differs from the parent molecule in that the polar residue, serine, occupying position 9 in the B chain and located on the outside of the insulin molecule, has been replaced with the hydrophobic leucine residue. For the synthesis of this analogue the [Leu9]B chain of human insulin was chemically synthesized by the fragment condensation approach and isolated in the S-sulfonated form. Combination of this compound with the sulfhydryl form of human A chain afforded [Leu9-B]insulin. Separation of this analogue from the combination mixture and isolation as the hydrochloride in purified form were accomplished by chromatography on a carboxymethylcellulose column with an acetate buffer (pH 3.3) and an exponential sodium chloride gradient. [Leu9-B]Insulin possesses a potency of 13-14 IU/mg when assayed by the mouse convulsion method and 11-12 IU/mg by the radioimmunoassay method as compared to 23-25 IU/mg possessed by the natural hormone.", "contents": "Synthesis of human [9-leucine-B] Insulin. The synthesis and isolation in purified form of [Leu9-B]insulin, a biologically active analogue of human insulin, is described. This analogue differs from the parent molecule in that the polar residue, serine, occupying position 9 in the B chain and located on the outside of the insulin molecule, has been replaced with the hydrophobic leucine residue. For the synthesis of this analogue the [Leu9]B chain of human insulin was chemically synthesized by the fragment condensation approach and isolated in the S-sulfonated form. Combination of this compound with the sulfhydryl form of human A chain afforded [Leu9-B]insulin. Separation of this analogue from the combination mixture and isolation as the hydrochloride in purified form were accomplished by chromatography on a carboxymethylcellulose column with an acetate buffer (pH 3.3) and an exponential sodium chloride gradient. [Leu9-B]Insulin possesses a potency of 13-14 IU/mg when assayed by the mouse convulsion method and 11-12 IU/mg by the radioimmunoassay method as compared to 23-25 IU/mg possessed by the natural hormone.", "PMID": 963021}
{"id": "pubmed23n0032_5327", "title": "Synthesis of two biologically active insulin analogues with modifications at the N-terminal and N- and C-terminal amino acid residues.", "content": "The synthesis and isolation in purified form of two analogues of insulin is described. [21-Isoasparagine-A] ([Iasn21-A]) insulin differs from the parent molecule in that the amino acid residue, asparagine, found at the C terminus of the A chain (A21) has been replaced by isoasparagine. [Sar1, Iasn21-A] insulin differs from insulin in that both the A1 and A21 amino acid residues, glycine and asparagine, have been substituted by sarcosine and isoasparagine, respectively. The synthesis of these analogues followed the pattern employed in this laboratory for the synthesis of insulin and its analogues. The S-sulfonated derivatives of the A chain analogues were chemically synthesized, converted to their sulfhydryl forms, and then combined with the S-sulfonated B chain to produce the respective insulin analogues. Isolation of the insulin analogues from the combination mixtures was effected by chromatography on a carboxymethylcellulose column with an exponential sodium chloride gradient. By the mouse convulsion assay method [Iasn21-A]insulin possessed a potency of 21 IU/mg and [Sar1, Iasn21-A] insulin 15 IU/mg. The radioimmunoassay method gave values of 16 IU/mg for the former and 7IU/mg for the altter analogue. The natural hormone has a potency of 23-25 IU/mg by both assay methods. These data indicate that the alpha- and beta-carboxyl groups of the A21 amino acid residue are nearly equivalent in terms of their contribution to the expression of the biological activity of insulin. Furthermore, these data strengthen the speculation (Cosmatos, A., Johnson, S., Breier, B., and Katsoyannis, P. G. (1975), J. Chem. Soc. Perkin Trans. 1, 2157) that the change in the relative positive charge at the N-terminal amino acid residue of the A chain is responsible for the considerable decrease in the immunoreactivity observed in such modified insulins.", "contents": "Synthesis of two biologically active insulin analogues with modifications at the N-terminal and N- and C-terminal amino acid residues. The synthesis and isolation in purified form of two analogues of insulin is described. [21-Isoasparagine-A] ([Iasn21-A]) insulin differs from the parent molecule in that the amino acid residue, asparagine, found at the C terminus of the A chain (A21) has been replaced by isoasparagine. [Sar1, Iasn21-A] insulin differs from insulin in that both the A1 and A21 amino acid residues, glycine and asparagine, have been substituted by sarcosine and isoasparagine, respectively. The synthesis of these analogues followed the pattern employed in this laboratory for the synthesis of insulin and its analogues. The S-sulfonated derivatives of the A chain analogues were chemically synthesized, converted to their sulfhydryl forms, and then combined with the S-sulfonated B chain to produce the respective insulin analogues. Isolation of the insulin analogues from the combination mixtures was effected by chromatography on a carboxymethylcellulose column with an exponential sodium chloride gradient. By the mouse convulsion assay method [Iasn21-A]insulin possessed a potency of 21 IU/mg and [Sar1, Iasn21-A] insulin 15 IU/mg. The radioimmunoassay method gave values of 16 IU/mg for the former and 7IU/mg for the altter analogue. The natural hormone has a potency of 23-25 IU/mg by both assay methods. These data indicate that the alpha- and beta-carboxyl groups of the A21 amino acid residue are nearly equivalent in terms of their contribution to the expression of the biological activity of insulin. Furthermore, these data strengthen the speculation (Cosmatos, A., Johnson, S., Breier, B., and Katsoyannis, P. G. (1975), J. Chem. Soc. Perkin Trans. 1, 2157) that the change in the relative positive charge at the N-terminal amino acid residue of the A chain is responsible for the considerable decrease in the immunoreactivity observed in such modified insulins.", "PMID": 963022}
{"id": "pubmed23n0032_5328", "title": "Synthetic, cross-linked polypentapeptide fo tropoelastin: an anisotropic, fibrillar elastomer.", "content": "Physical properties of the cross-linked polypentapeptide of tropoelastin are reported along with chemical characterization of key intermediates in its synthesis. 220 MHz proton magnetic resonance spectra are reported on the constituent pentamers and their respective high polymers which verify structural and conformational integrity. Scanning electron microscopy of the cross-linked material formed without orientation and with flow orientation is reported. The former demonstrates the inherent fibrillar and anisotropic nature of the synthetic product. Stress-strain studies show the cross-linked polypentapeptide to exhibit elastomeric properties that are dependent on the water content of the matrix. At high water contents the elastic modulus is less than that of wet native aortic elastin and becomes greater on drying.", "contents": "Synthetic, cross-linked polypentapeptide fo tropoelastin: an anisotropic, fibrillar elastomer. Physical properties of the cross-linked polypentapeptide of tropoelastin are reported along with chemical characterization of key intermediates in its synthesis. 220 MHz proton magnetic resonance spectra are reported on the constituent pentamers and their respective high polymers which verify structural and conformational integrity. Scanning electron microscopy of the cross-linked material formed without orientation and with flow orientation is reported. The former demonstrates the inherent fibrillar and anisotropic nature of the synthetic product. Stress-strain studies show the cross-linked polypentapeptide to exhibit elastomeric properties that are dependent on the water content of the matrix. At high water contents the elastic modulus is less than that of wet native aortic elastin and becomes greater on drying.", "PMID": 963023}
{"id": "pubmed23n0032_5329", "title": "Cross-linked polypentapeptide of tropoelastin: an insoluble, serum calcifiable matrix.", "content": "The synthetic, cross-linked polypentapeptide of tropoelastin has been shown to calcify from serum alone even when separated from the serum medium by a dialysis membrane with a low-molecular-weight cut off. By microprobe analysis, it appeared that the only serum elements required for the calcification were calcium and phosphorus. Furthermore, thin sections of the calcified matrix showed the calcification to occur throughout the matrix, and thereby verified that it is a bulk property of the matrix and not an interfacial property. To our knowledge this is the first demonstration of an insoluble, synthetic polypeptide to function as a serum calcifiable matrix and by doing so it opens the door to potential medical applications.", "contents": "Cross-linked polypentapeptide of tropoelastin: an insoluble, serum calcifiable matrix. The synthetic, cross-linked polypentapeptide of tropoelastin has been shown to calcify from serum alone even when separated from the serum medium by a dialysis membrane with a low-molecular-weight cut off. By microprobe analysis, it appeared that the only serum elements required for the calcification were calcium and phosphorus. Furthermore, thin sections of the calcified matrix showed the calcification to occur throughout the matrix, and thereby verified that it is a bulk property of the matrix and not an interfacial property. To our knowledge this is the first demonstration of an insoluble, synthetic polypeptide to function as a serum calcifiable matrix and by doing so it opens the door to potential medical applications.", "PMID": 963024}
{"id": "pubmed23n0032_5330", "title": "Hybridization of maize chloroplast DNA with transfer ribonucleic acids.", "content": "Hybridization of [125I] tRNA to chloroplast DNA indicates that 0.60-0.75% of maize chloroplast DNA contains sequences complementary to maize tRNA, corresponding to 20-26 tRNA cistrons. Green maize seedlings contain about twice the amount of chloroplast DNA-hybridizable tRNA as etiolated maize seedings. tRNA from green or etiolated maize seedlings was also aminoacylated in vitro with 21 labeled amino acids and then incubated with filters containing chloroplast DNA, tRNAs charging a total of at least 16 different amino acids hybridized with chloroplast DNA. Most of these plastid aminoacyl-tRNAs were present in higher concentrations in tRNA isolated from green maize seedlings, although there were several exceptions. The results are consistent with the hypothesis that a complete or nearly complete set or tRNAs can be transcribed from chloroplast DNA.", "contents": "Hybridization of maize chloroplast DNA with transfer ribonucleic acids. Hybridization of [125I] tRNA to chloroplast DNA indicates that 0.60-0.75% of maize chloroplast DNA contains sequences complementary to maize tRNA, corresponding to 20-26 tRNA cistrons. Green maize seedlings contain about twice the amount of chloroplast DNA-hybridizable tRNA as etiolated maize seedings. tRNA from green or etiolated maize seedlings was also aminoacylated in vitro with 21 labeled amino acids and then incubated with filters containing chloroplast DNA, tRNAs charging a total of at least 16 different amino acids hybridized with chloroplast DNA. Most of these plastid aminoacyl-tRNAs were present in higher concentrations in tRNA isolated from green maize seedlings, although there were several exceptions. The results are consistent with the hypothesis that a complete or nearly complete set or tRNAs can be transcribed from chloroplast DNA.", "PMID": 963025}
{"id": "pubmed23n0032_5331", "title": "Poly(adenylic acid)-containing RNA from plastids of maize.", "content": "Hybridization experiments with chloroplast DNA and 125I-labeled RNA from maize seedlings suggest that chloroplasts and etioplasts contain detectable amounts of RNA that contains poly(adenylic aicd) (poly (A)) and was transcribed from chloroplast DNA. About 6% of the total poly(A)-containing RNA isolated from maize seedlings hybridized to chloroplast DNA. Poly(A)-containing RNA could also be isolated directly from purified chloroplasts that were treated with ribonucleases to reduce cytoplasmic contamination. At least 65% of this poly(A)-containing RNA hybridized to chloroplast DNA. Chloroplast poly(A) tracts average about 45 nucleotides in length, one-half the average size of poly(A) tracts from whole cells. The poly (A) tracts themselves are probably added to plastid RNAs following their transcription, because maize chloroplast DNA was found not to contain poly(dT).", "contents": "Poly(adenylic acid)-containing RNA from plastids of maize. Hybridization experiments with chloroplast DNA and 125I-labeled RNA from maize seedlings suggest that chloroplasts and etioplasts contain detectable amounts of RNA that contains poly(adenylic aicd) (poly (A)) and was transcribed from chloroplast DNA. About 6% of the total poly(A)-containing RNA isolated from maize seedlings hybridized to chloroplast DNA. Poly(A)-containing RNA could also be isolated directly from purified chloroplasts that were treated with ribonucleases to reduce cytoplasmic contamination. At least 65% of this poly(A)-containing RNA hybridized to chloroplast DNA. Chloroplast poly(A) tracts average about 45 nucleotides in length, one-half the average size of poly(A) tracts from whole cells. The poly (A) tracts themselves are probably added to plastid RNAs following their transcription, because maize chloroplast DNA was found not to contain poly(dT).", "PMID": 963026}
{"id": "pubmed23n0032_5332", "title": "Inverted repetitive sequences in the human genome.", "content": "A specific class of DNA sequences, the inverted repetitive sequences, forms hairpin-like structures in denatured DNA by the folding back of a single linear chain. The reassociation process of these sequences is unimolecular and the rate is extremely fast. Inverted repetitive sequences comprise 6% of the total human genome. They appear to be heterogeneous in length with an overall average length of 190 nucleotides. The inverted sequences are represented in almost all families of repetition frequencies, highly repetitive as well as very few copies per genome. They are not localized at unique sites on metaphase chromosomes. It is estimated that there are approximately 2 X 10(6) inverted repeats per haploid human genome. The biological function of this class of sequences is unknown.", "contents": "Inverted repetitive sequences in the human genome. A specific class of DNA sequences, the inverted repetitive sequences, forms hairpin-like structures in denatured DNA by the folding back of a single linear chain. The reassociation process of these sequences is unimolecular and the rate is extremely fast. Inverted repetitive sequences comprise 6% of the total human genome. They appear to be heterogeneous in length with an overall average length of 190 nucleotides. The inverted sequences are represented in almost all families of repetition frequencies, highly repetitive as well as very few copies per genome. They are not localized at unique sites on metaphase chromosomes. It is estimated that there are approximately 2 X 10(6) inverted repeats per haploid human genome. The biological function of this class of sequences is unknown.", "PMID": 963027}
{"id": "pubmed23n0032_5333", "title": "Histone-histone interactions within chromatin. Preliminary characterization of presumptive H2B-H2A and H2B-H4 binding.", "content": "Cyanogen bromide peptide analyses of cross-linked histones show that histone 2B possesses separate binding sites for histones 2A and 4. Various polymorphic forms of H2A and H4 in chromatin are bound to H2B through these binding sites. These interactions are thus a fundamental structural feature of chromatin. Additional data strongly suggest that H2B is bound to H2A and H4 simultaneously in vivo. Mouse L cells were treated with either tetranitromethane (TNM) or ultraviolet (uv) light at 280 nm in order to induce the cross-linking of histone 2B in their chromatin to either histone 4 or 2A. The characteristics of these cross-linking agents and the reactions they induce suggest that the cross-links define loci within the actual binding sites between these histones. Characterization of the cyanogen bromide peptide fragments of the H2B-H4 and H2B-H2A dimers showed that the C terminal half of H2B contains the link to H4 (also near its C terminus), whereas the N-terminal half contains the link to H2A. Treatment of the cells with both uv light and TNM results in the appearance of a single new trimer which we presume to be H2A-H2B-H4. We conclude form this that H2B interacts simultaneously with H2A and H4 in chromatin through separate binding sites. Further analysis shows that several acetylated subspecies of H4 are represented in the H2B-H4 dimer and that at least two of the polymorphic forms of H2A are present in the H2B-H2A dimer. These data reveal that the H2A-H2B-H4 association is an important underlying structural feature of chromatin.", "contents": "Histone-histone interactions within chromatin. Preliminary characterization of presumptive H2B-H2A and H2B-H4 binding. Cyanogen bromide peptide analyses of cross-linked histones show that histone 2B possesses separate binding sites for histones 2A and 4. Various polymorphic forms of H2A and H4 in chromatin are bound to H2B through these binding sites. These interactions are thus a fundamental structural feature of chromatin. Additional data strongly suggest that H2B is bound to H2A and H4 simultaneously in vivo. Mouse L cells were treated with either tetranitromethane (TNM) or ultraviolet (uv) light at 280 nm in order to induce the cross-linking of histone 2B in their chromatin to either histone 4 or 2A. The characteristics of these cross-linking agents and the reactions they induce suggest that the cross-links define loci within the actual binding sites between these histones. Characterization of the cyanogen bromide peptide fragments of the H2B-H4 and H2B-H2A dimers showed that the C terminal half of H2B contains the link to H4 (also near its C terminus), whereas the N-terminal half contains the link to H2A. Treatment of the cells with both uv light and TNM results in the appearance of a single new trimer which we presume to be H2A-H2B-H4. We conclude form this that H2B interacts simultaneously with H2A and H4 in chromatin through separate binding sites. Further analysis shows that several acetylated subspecies of H4 are represented in the H2B-H4 dimer and that at least two of the polymorphic forms of H2A are present in the H2B-H2A dimer. These data reveal that the H2A-H2B-H4 association is an important underlying structural feature of chromatin.", "PMID": 963028}
{"id": "pubmed23n0032_5334", "title": "An experimental method correcting for absorption flattening and scattering in suspensions of absorbing particles: circular dichroism and absorption spectra of hemoglobin in situ in red blood cells.", "content": "An experimental approach to interpretation of the anomalous absorption and circular dichroism (CD) spectra of hemoglobin in situ in red blood cells is reported. Absorption flattening effects have been overcome by use of high cell concentratons in very short light path cuvettes. Differential scattering contributions to circular dichroism have been resolved using a CD instrument capable of variable detection geometry. Scattering effects have also been resolved using media of high refractive index to match that of the red blood cell. The results are in agreement with a parellel calculational analysis of red blood cell CD spectra, which predicted the relative magnitudes of the flattening and differential scattering CD contributions. An experimental absorption spectrum has been obtained for hemoglobin in the red blood cell with scattering and flattening eliminated. This quantitatively simulates the spectrum of a hemoglobin solution. The methods described should be widely applicable to conformational studies of macromolecules in their native environment.", "contents": "An experimental method correcting for absorption flattening and scattering in suspensions of absorbing particles: circular dichroism and absorption spectra of hemoglobin in situ in red blood cells. An experimental approach to interpretation of the anomalous absorption and circular dichroism (CD) spectra of hemoglobin in situ in red blood cells is reported. Absorption flattening effects have been overcome by use of high cell concentratons in very short light path cuvettes. Differential scattering contributions to circular dichroism have been resolved using a CD instrument capable of variable detection geometry. Scattering effects have also been resolved using media of high refractive index to match that of the red blood cell. The results are in agreement with a parellel calculational analysis of red blood cell CD spectra, which predicted the relative magnitudes of the flattening and differential scattering CD contributions. An experimental absorption spectrum has been obtained for hemoglobin in the red blood cell with scattering and flattening eliminated. This quantitatively simulates the spectrum of a hemoglobin solution. The methods described should be widely applicable to conformational studies of macromolecules in their native environment.", "PMID": 963029}
{"id": "pubmed23n0032_5335", "title": "The role of methionine-192 of the chymotrypsin active site in the binding and catalysis of mono(amino acid) and peptide substrates.", "content": "We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulfoxide)-192-delta-chymotrypsin (MS-delta-Cht) shows a four- to fivefold decrease in kcat and unchanged Km(app) with respect to delta-chymotrypsin. Hydrolysis of alpha-casein by MS-delta-Cht shows a similar 4.2-fold decrease in kcat. These results imply that the Met-192 acts differently with substrates that bind only in the primary, S1, binding site (i.e., AcPheNH2) from those that bind to more extended regions of the enzyme active site. In the binding of c+AcPheNH2  and AcTrpNH2, the results support a mechanism in which the Met-192 acts to slow the rate of sustrate dissociation from the Michaelis complex to free substrate and enzyme. This is in agreement with the x-ray crystallographic structure of dioxane inhibited alpha-chymotrypsin (Steitz, T., et al. (1969), J. Mol. Biol. 46, 337). However, this mechanism is not apparent when peptide and protein substrates bind. The decrease in kcat on Met-192 modification of approximately fivefold in the hydrolysis of polypeptide substrates show a small, but significant, catalytic contribution of the Met-192 toward the lowering of the energy of activation polypeptide substrate hydrolysis by chymotrypsin. This may support the crystallographic model of Fersht et al. (Fersht, A., et al. (1973), Biochemistry 12, 2035) in which it is proposed that the Met-192 participates in the distortion of bound polypeptide substrates toward the reaction transition-state configuration and, thus, plays a role in catalysis. However, if this mechanism occurs, the effect is small, only contributing about 1 kcal/mol to the lowering of the reaction activation energy.", "contents": "The role of methionine-192 of the chymotrypsin active site in the binding and catalysis of mono(amino acid) and peptide substrates. We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulfoxide)-192-delta-chymotrypsin (MS-delta-Cht) shows a four- to fivefold decrease in kcat and unchanged Km(app) with respect to delta-chymotrypsin. Hydrolysis of alpha-casein by MS-delta-Cht shows a similar 4.2-fold decrease in kcat. These results imply that the Met-192 acts differently with substrates that bind only in the primary, S1, binding site (i.e., AcPheNH2) from those that bind to more extended regions of the enzyme active site. In the binding of c+AcPheNH2  and AcTrpNH2, the results support a mechanism in which the Met-192 acts to slow the rate of sustrate dissociation from the Michaelis complex to free substrate and enzyme. This is in agreement with the x-ray crystallographic structure of dioxane inhibited alpha-chymotrypsin (Steitz, T., et al. (1969), J. Mol. Biol. 46, 337). However, this mechanism is not apparent when peptide and protein substrates bind. The decrease in kcat on Met-192 modification of approximately fivefold in the hydrolysis of polypeptide substrates show a small, but significant, catalytic contribution of the Met-192 toward the lowering of the energy of activation polypeptide substrate hydrolysis by chymotrypsin. This may support the crystallographic model of Fersht et al. (Fersht, A., et al. (1973), Biochemistry 12, 2035) in which it is proposed that the Met-192 participates in the distortion of bound polypeptide substrates toward the reaction transition-state configuration and, thus, plays a role in catalysis. However, if this mechanism occurs, the effect is small, only contributing about 1 kcal/mol to the lowering of the reaction activation energy.", "PMID": 963030}
{"id": "pubmed23n0032_5336", "title": "Histidine decarboxylase of Lactobacillus 30a: inactivation and active-site labeling by L-histidine methyl ester.", "content": "Histidine decarboxylase from Lactobacillus 30a is rapidly and irreversibly inactivated upon incubation with L-histidine methyl ester. The rate of inactivation is first-order with respect to remaining active enzyme and exhibits saturation kinetics with a kinact of 1.2 mM and an apparent first-order rate constant of 0.346 min-1 at pH 4.8 and 25 degrees C. On complete inactivation, 3 mol of [14C]histidine (from L-[14C]histidine methyl ester) and 2 mol of 14C (from L-histidine [14C]methyl ester) are bound in nondialyzable form per mol (190 000 g) of protein inactivated with a corresponding loss of three of the five DTNB-titratable--SH groups that are essential for activity of the native enzyme. Imidazole propionate, a competitive inhibitor of the enzyme, protects against inactivation, loss of --SH groups, and incorporation of radioactivity from both the histidine and the methyl ester moieties of the labeled inhibitor, and kinetic evidence indicates that imidazole propionate and histidine methyl ester compete for binding at the active site of histidine decarboxylase in a mutually exclusive manner. Treatment of the labeled protein with either alkali or hydroxylamine results in the quantitative release of radioactivity. These data suggest that inactivation of histidine decarboxylase by L-histidine methyl ester results from two different modes of interaction between the inhibitor and the active site of histidine decarboxylase; the major interaction involves an essential -SH group.", "contents": "Histidine decarboxylase of Lactobacillus 30a: inactivation and active-site labeling by L-histidine methyl ester. Histidine decarboxylase from Lactobacillus 30a is rapidly and irreversibly inactivated upon incubation with L-histidine methyl ester. The rate of inactivation is first-order with respect to remaining active enzyme and exhibits saturation kinetics with a kinact of 1.2 mM and an apparent first-order rate constant of 0.346 min-1 at pH 4.8 and 25 degrees C. On complete inactivation, 3 mol of [14C]histidine (from L-[14C]histidine methyl ester) and 2 mol of 14C (from L-histidine [14C]methyl ester) are bound in nondialyzable form per mol (190 000 g) of protein inactivated with a corresponding loss of three of the five DTNB-titratable--SH groups that are essential for activity of the native enzyme. Imidazole propionate, a competitive inhibitor of the enzyme, protects against inactivation, loss of --SH groups, and incorporation of radioactivity from both the histidine and the methyl ester moieties of the labeled inhibitor, and kinetic evidence indicates that imidazole propionate and histidine methyl ester compete for binding at the active site of histidine decarboxylase in a mutually exclusive manner. Treatment of the labeled protein with either alkali or hydroxylamine results in the quantitative release of radioactivity. These data suggest that inactivation of histidine decarboxylase by L-histidine methyl ester results from two different modes of interaction between the inhibitor and the active site of histidine decarboxylase; the major interaction involves an essential -SH group.", "PMID": 963031}
{"id": "pubmed23n0032_5337", "title": "Maleylacetone cis-trans-isomerase: affinity chromatography on glutathione-bound sepharose. Two-substrate-binding sequence from inhibition patterns.", "content": "Maleylacetone cis-trans-isomerase isolated from Vibrio 01 binds glutathione strongly; Km = 1.4 X 10(-4) M. Oxidized glutathione and S-methylglutathione are competitive inhibitors, KI = 9.4 X 10(-4) and 1.2 X 10(-3) M, respectively. Based on these interactions, three different glutathione-bound agarose affinity adsorbents were synthesized and tested. Affinity chromatography of the isomerase with one of these affords 70- to 100-fold purifications. In separate syntheses, portions of the affinity arm were prepared and examined as to their inhibitory properties in the enzyme-catalyzed reaction. The fragment, containing glutathione bound through its sulfur to the carbon chain, is a powerful competitive inhibitor for glutathione (KI = 6 X 10(-5) M). The results described suggest that the isomerase binds glutathione through the backbone of the tripeptide and that the thiol group is required for activity. The initial velocity patterns of the enzyme-catalyzed reaction resulting from simultaneous variation of glutathione and maleylacetone concentrations were examined in the absence and presence of inhibitors resembling glutathione. The observed kinetic patterns suggest an ordered sequence of binding maleylacetone first followed by glutathione.", "contents": "Maleylacetone cis-trans-isomerase: affinity chromatography on glutathione-bound sepharose. Two-substrate-binding sequence from inhibition patterns. Maleylacetone cis-trans-isomerase isolated from Vibrio 01 binds glutathione strongly; Km = 1.4 X 10(-4) M. Oxidized glutathione and S-methylglutathione are competitive inhibitors, KI = 9.4 X 10(-4) and 1.2 X 10(-3) M, respectively. Based on these interactions, three different glutathione-bound agarose affinity adsorbents were synthesized and tested. Affinity chromatography of the isomerase with one of these affords 70- to 100-fold purifications. In separate syntheses, portions of the affinity arm were prepared and examined as to their inhibitory properties in the enzyme-catalyzed reaction. The fragment, containing glutathione bound through its sulfur to the carbon chain, is a powerful competitive inhibitor for glutathione (KI = 6 X 10(-5) M). The results described suggest that the isomerase binds glutathione through the backbone of the tripeptide and that the thiol group is required for activity. The initial velocity patterns of the enzyme-catalyzed reaction resulting from simultaneous variation of glutathione and maleylacetone concentrations were examined in the absence and presence of inhibitors resembling glutathione. The observed kinetic patterns suggest an ordered sequence of binding maleylacetone first followed by glutathione.", "PMID": 963032}
{"id": "pubmed23n0032_5338", "title": "Interactions between the subfractons of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2.", "content": "The nonhistone chromosomal proteins, HMG1 and HMG2, interact with the various subfractions of calf thymus H1 with a high degree of specificity. Subfractions 1b and 2 interact very strongly with HMG1 to form heterodimers. In contrast, subfractions 3a and 3b interact much more weakly. HMG2 interacts with 3a and 3b but shows no detectable complexing with 1a and 2.", "contents": "Interactions between the subfractons of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. The nonhistone chromosomal proteins, HMG1 and HMG2, interact with the various subfractions of calf thymus H1 with a high degree of specificity. Subfractions 1b and 2 interact very strongly with HMG1 to form heterodimers. In contrast, subfractions 3a and 3b interact much more weakly. HMG2 interacts with 3a and 3b but shows no detectable complexing with 1a and 2.", "PMID": 963033}
{"id": "pubmed23n0032_5339", "title": "Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules.", "content": "The interactions of nucleotides and their role in the polymerization of tubulin have been studied in detail. GTP promotes polymerization by binding to the exchangeable site (E site) of tubulin. The microtubules formed contain only GDP at the E site, indicating that hydrolysis of E site GTP occurs during or shortly after polymerization. Tubulin prepared by several cycles of polymerization and depolymerization will polymerize in the presence of ATP as well as GTP. Polymerization in ATP is preceded by a distinct lag period which is shorter at higher concentrations of ATP. As reported by others ATP will transphosphorylate bound GDP to GTP. Under polymerizing conditions the maximum level of GTP formation occurs at about the same time as the onset of polymerization, and the lag probably reflects the time necessary to transphosphorylate a critical concentration of tubulin. The transphosphorylated protein can be isolated and will polymerize without further addition of nucleotide. The transphosphorylated GTP is hydrolyzed and the phosphate released during polymerization. About 25% of the phosphate transferred from ATP is noncovalently bound to the subunit as inorganic phosphate and this fraction is also released during polymerization. The nonhydrolyzable analogue of GTP, GMPPNP, will promote microtubule assembly at high concentration. GMPPNP assembled microtubules do not depolymerize in Ca concentrations several fold greater than that which will completely depolymerize GTP assembled tubules; however, addition of Ca prior to inducing polymerization in GMPPNP prevents the formation of microtubules. Thus GTP hydrolysis appears to promote depolymerization rather than polymerization. GDP does not promote microtubule assembly but can inhibit GTP binding and GTP induced polymerization. GDP does not, however, induce the depolymerization of formed microtubules. These experiments demonstrate that tubulin polymerization can not be treated as a thermodynamically reversible process, but must involve one or more irreversible steps. Exchange experiments with [3H]GTP indicate that the \"E\" site on both microtubules and ring aggregates of tubulin is blocked and does not exchange rapidly. However, during polymerization and depolymerization induced by raising or lowering the temperature, respectively, all the E sites become transiently available and will exchange their nucleotide. This observation does not suggest a direct morphological transition between rings and microtubules. The presence of a blocked E site on the rings explains the apparent transphosphorylation and hydrolysis of \"N\" site nucleotide reported by others.", "contents": "Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules. The interactions of nucleotides and their role in the polymerization of tubulin have been studied in detail. GTP promotes polymerization by binding to the exchangeable site (E site) of tubulin. The microtubules formed contain only GDP at the E site, indicating that hydrolysis of E site GTP occurs during or shortly after polymerization. Tubulin prepared by several cycles of polymerization and depolymerization will polymerize in the presence of ATP as well as GTP. Polymerization in ATP is preceded by a distinct lag period which is shorter at higher concentrations of ATP. As reported by others ATP will transphosphorylate bound GDP to GTP. Under polymerizing conditions the maximum level of GTP formation occurs at about the same time as the onset of polymerization, and the lag probably reflects the time necessary to transphosphorylate a critical concentration of tubulin. The transphosphorylated protein can be isolated and will polymerize without further addition of nucleotide. The transphosphorylated GTP is hydrolyzed and the phosphate released during polymerization. About 25% of the phosphate transferred from ATP is noncovalently bound to the subunit as inorganic phosphate and this fraction is also released during polymerization. The nonhydrolyzable analogue of GTP, GMPPNP, will promote microtubule assembly at high concentration. GMPPNP assembled microtubules do not depolymerize in Ca concentrations several fold greater than that which will completely depolymerize GTP assembled tubules; however, addition of Ca prior to inducing polymerization in GMPPNP prevents the formation of microtubules. Thus GTP hydrolysis appears to promote depolymerization rather than polymerization. GDP does not promote microtubule assembly but can inhibit GTP binding and GTP induced polymerization. GDP does not, however, induce the depolymerization of formed microtubules. These experiments demonstrate that tubulin polymerization can not be treated as a thermodynamically reversible process, but must involve one or more irreversible steps. Exchange experiments with [3H]GTP indicate that the \"E\" site on both microtubules and ring aggregates of tubulin is blocked and does not exchange rapidly. However, during polymerization and depolymerization induced by raising or lowering the temperature, respectively, all the E sites become transiently available and will exchange their nucleotide. This observation does not suggest a direct morphological transition between rings and microtubules. The presence of a blocked E site on the rings explains the apparent transphosphorylation and hydrolysis of \"N\" site nucleotide reported by others.", "PMID": 963034}
{"id": "pubmed23n0032_5340", "title": "Protein rotational relaxation as studied by solvent 1H and 2H magnetic relaxation.", "content": "Earlier studies of the magnetic field dependence of the nuclear spin magnetic relaxation rate of solvent protons in solutions of diamagnetic proteins have indicated that this dependence (called relaxation dispersion) is related to the rotational Brownian motion of solute proteins. In essence, the dispersion is such that 1/T1 (the proton spin-lattice relaxation rate) decreases monotonically as the magnetic field is increased from a very low value (approximately 10 Oe); the dispersion has a point of inflection at a value of magnetic field which depends on protein size, shape, concentration, temperature, and solvent composition. The value of the proton Larmor precession frequency nu(c) at the inflection field appears to relate to tau (R), the rotational relaxation time of the protein molecules. We have measured proton relaxation dispersions for solutions of various proteins that span a three-decade range of molecular weights, and for one sample of transfer ribonucleic acid. We have also measured deuteron relaxation dispersions for solutions of three proteins: lysozyme, carbonmonoxyhemoglobin, and Helix pomatia hemocyanin with molecular weight 900 000. A quantitative relationship between both proton and deuteron dispersion data and protein rotational relaxation is confirmed, and the point is made that magnetic dispersion measurements are of very general applicability for measuring the rotational relaxation rate of macromolecules in solution. It has been previously shown that the influence of proton motion on the relaxation behavior of the solvent is not due to exchange of solvent molecules between the bulk solvent and a hydration region of the protein. In the present paper, we suggest that the interaction results from a long range hydrodynamic effect fundamental to the situation of large Brownian particles in an essentially continuum fluid. The general features of the proposed mechanism are indicated, but no theoretical computations are presented.", "contents": "Protein rotational relaxation as studied by solvent 1H and 2H magnetic relaxation. Earlier studies of the magnetic field dependence of the nuclear spin magnetic relaxation rate of solvent protons in solutions of diamagnetic proteins have indicated that this dependence (called relaxation dispersion) is related to the rotational Brownian motion of solute proteins. In essence, the dispersion is such that 1/T1 (the proton spin-lattice relaxation rate) decreases monotonically as the magnetic field is increased from a very low value (approximately 10 Oe); the dispersion has a point of inflection at a value of magnetic field which depends on protein size, shape, concentration, temperature, and solvent composition. The value of the proton Larmor precession frequency nu(c) at the inflection field appears to relate to tau (R), the rotational relaxation time of the protein molecules. We have measured proton relaxation dispersions for solutions of various proteins that span a three-decade range of molecular weights, and for one sample of transfer ribonucleic acid. We have also measured deuteron relaxation dispersions for solutions of three proteins: lysozyme, carbonmonoxyhemoglobin, and Helix pomatia hemocyanin with molecular weight 900 000. A quantitative relationship between both proton and deuteron dispersion data and protein rotational relaxation is confirmed, and the point is made that magnetic dispersion measurements are of very general applicability for measuring the rotational relaxation rate of macromolecules in solution. It has been previously shown that the influence of proton motion on the relaxation behavior of the solvent is not due to exchange of solvent molecules between the bulk solvent and a hydration region of the protein. In the present paper, we suggest that the interaction results from a long range hydrodynamic effect fundamental to the situation of large Brownian particles in an essentially continuum fluid. The general features of the proposed mechanism are indicated, but no theoretical computations are presented.", "PMID": 963035}
{"id": "pubmed23n0032_5341", "title": "Conformational properties of the complexes formed by proteins and sodium dodecyl sulfate.", "content": "Circular dichroism spectra have been obtained for albumin, alpha-chymotrypsinogen, collagen, concanavalin A, elastase, hemoglobin, histone f2b, alpha-lactalbumin, lactate dehydrogenase, beta-lactoglobulin, lysozyme, myoglobin, papain, ribonuclease A, and thermolysin in the presence of sodium dodecyl sulfate and dithiothreitol. While all spectra have the shape anticipated for a mixture of random coil and alpha helix, the intensities differ markedly ([theta]222 ranges from --1400 to --15 000 deg cm2/dmol). The variation in the circular dichroism can be quantitatively explained by a model which assumes that the arginyl, histidyl, and lysyl residues have an enhanced probability of propagating a helical segment in the presence of the detergent. The model also permits the computation of dimensional properties (unperturbed end-to-end distance and radius of gyration) for polypeptides of known amino acid sequence. Such computations have been performed for 67 proteins. The computed dimensions are compatible with experimental values and with the molecular weight dependence of the transport properties of the complexes. Furthermore, the model can account for the abnormal transport properties of the sodium dodecyl sulfate complexes formed by ribonuclease A, collagen fragments, and histones f2a1, f2a2, f2b, and f3. Even though some of the protein--sodium dodecyl sulfate complexes have helical contents as high as 50%, their overall conformation more closely approximates that of a random coil than a rod.", "contents": "Conformational properties of the complexes formed by proteins and sodium dodecyl sulfate. Circular dichroism spectra have been obtained for albumin, alpha-chymotrypsinogen, collagen, concanavalin A, elastase, hemoglobin, histone f2b, alpha-lactalbumin, lactate dehydrogenase, beta-lactoglobulin, lysozyme, myoglobin, papain, ribonuclease A, and thermolysin in the presence of sodium dodecyl sulfate and dithiothreitol. While all spectra have the shape anticipated for a mixture of random coil and alpha helix, the intensities differ markedly ([theta]222 ranges from --1400 to --15 000 deg cm2/dmol). The variation in the circular dichroism can be quantitatively explained by a model which assumes that the arginyl, histidyl, and lysyl residues have an enhanced probability of propagating a helical segment in the presence of the detergent. The model also permits the computation of dimensional properties (unperturbed end-to-end distance and radius of gyration) for polypeptides of known amino acid sequence. Such computations have been performed for 67 proteins. The computed dimensions are compatible with experimental values and with the molecular weight dependence of the transport properties of the complexes. Furthermore, the model can account for the abnormal transport properties of the sodium dodecyl sulfate complexes formed by ribonuclease A, collagen fragments, and histones f2a1, f2a2, f2b, and f3. Even though some of the protein--sodium dodecyl sulfate complexes have helical contents as high as 50%, their overall conformation more closely approximates that of a random coil than a rod.", "PMID": 963036}
{"id": "pubmed23n0032_5342", "title": "Nuclear estradiol receptor in the adult rat uterus: a new exchange assay.", "content": "A protamine exchange assay has been developed to measure uterine nuclear estrogen receptor in mature rats exposed to estradiol (E). After ovariectomized-adrenalectomized mature rats are injected with E, estrogen receptor (RnE) is extracted from uterine nuclei with 0.6 M potassium chloride, diluted, and quantitatively precipitated with protamine sulfate. The precipitate is subjected to a ligand exchange with radiolabeled estradiol (E), with or without unlabeled diethylstilbesterol, to determine nonspecific binding. At 37 degrees C complete exchange of E for E in RnE is observed at 2.5 h; virtually no receptor degradation occurs up to at least 5 h. Exchange does not occur at 4 degrees C. Using the protamine assay, the depletion of cytoplasmic estrogen receptor (Rc) and the accumulation of RnE were studied at various doses of E at specific time points. Increasing doses of E result in a decrease of Rc with an equal increase of RnE. At the highest dose of E (10 mug) Rc is completely depleted within 10 min, by 6 h it is 25% replenished, and by 24 h returns to slightly above control levels. Within 10 min after the injection, RnE increases to 80-90% of the original cytoplasmic level of receptor (approximately 2-3 pmol/mg of DNA or approximately 1.5 pmol/100 mg of uterus). At 6 h RnE is 75% depleted and it is completely absent at 24 h. The protamine assay permits precise quantitative studies of nuclear estrogen receptor and avoids the problems of receptor degradation and excessive nonspecific binding often found in exchange reactions at elevated temperatures.", "contents": "Nuclear estradiol receptor in the adult rat uterus: a new exchange assay. A protamine exchange assay has been developed to measure uterine nuclear estrogen receptor in mature rats exposed to estradiol (E). After ovariectomized-adrenalectomized mature rats are injected with E, estrogen receptor (RnE) is extracted from uterine nuclei with 0.6 M potassium chloride, diluted, and quantitatively precipitated with protamine sulfate. The precipitate is subjected to a ligand exchange with radiolabeled estradiol (E), with or without unlabeled diethylstilbesterol, to determine nonspecific binding. At 37 degrees C complete exchange of E for E in RnE is observed at 2.5 h; virtually no receptor degradation occurs up to at least 5 h. Exchange does not occur at 4 degrees C. Using the protamine assay, the depletion of cytoplasmic estrogen receptor (Rc) and the accumulation of RnE were studied at various doses of E at specific time points. Increasing doses of E result in a decrease of Rc with an equal increase of RnE. At the highest dose of E (10 mug) Rc is completely depleted within 10 min, by 6 h it is 25% replenished, and by 24 h returns to slightly above control levels. Within 10 min after the injection, RnE increases to 80-90% of the original cytoplasmic level of receptor (approximately 2-3 pmol/mg of DNA or approximately 1.5 pmol/100 mg of uterus). At 6 h RnE is 75% depleted and it is completely absent at 24 h. The protamine assay permits precise quantitative studies of nuclear estrogen receptor and avoids the problems of receptor degradation and excessive nonspecific binding often found in exchange reactions at elevated temperatures.", "PMID": 963037}
{"id": "pubmed23n0032_5343", "title": "Synthesis and properties of poly(O6-methylguanylic acid) and poly(O6-ethylguanylic acid).", "content": "The nucleotide analogues, O6-methyl- and O6-ethylguanosine diphosphate, have been synthesized and polymerized to high-molecular-weight homopolymers with polynucleotide phosphorylase. The ultraviolet spectra of these polymers show marked hypochromicity, which suggests that they possess considerable secondary structures. Graphs of optical denisty vs. temperature in 0.15 M NaC1 indicate that cooperative melting occurs for both polymers, and that the secondary structure of poly(O6-methylguanosine monophosphate) is somewhat more stable than that of poly (O6-ethyl-guanosine monophosphate). Mixing experiments show that these analogue polymers no longer form helical structures with poly(C), nor do thye form helices with poly(U). We would conclude from these data that environmental mutagens and carcinogens which react at the O6 position of guanine not only disrupt normal base-pairing relationships, but may also affect the secondary structure of nucleic acids.", "contents": "Synthesis and properties of poly(O6-methylguanylic acid) and poly(O6-ethylguanylic acid). The nucleotide analogues, O6-methyl- and O6-ethylguanosine diphosphate, have been synthesized and polymerized to high-molecular-weight homopolymers with polynucleotide phosphorylase. The ultraviolet spectra of these polymers show marked hypochromicity, which suggests that they possess considerable secondary structures. Graphs of optical denisty vs. temperature in 0.15 M NaC1 indicate that cooperative melting occurs for both polymers, and that the secondary structure of poly(O6-methylguanosine monophosphate) is somewhat more stable than that of poly (O6-ethyl-guanosine monophosphate). Mixing experiments show that these analogue polymers no longer form helical structures with poly(C), nor do thye form helices with poly(U). We would conclude from these data that environmental mutagens and carcinogens which react at the O6 position of guanine not only disrupt normal base-pairing relationships, but may also affect the secondary structure of nucleic acids.", "PMID": 963038}
{"id": "pubmed23n0032_5344", "title": "Binding of platinum and palladium metallointercalation reagents and antitumor drugs to closed and open DNAs.", "content": "The interaction of platinum and palladium complexes with closed and nicked circular and linear DNAs was investigated by a variety of methods. Cationic metal complexes containing flat, aromatic ligands, such as 2,2',2''-terpyridine, o-phenanthroline, and 2,2'-bipyridine, interfere with the usual fluorescence enhancement of ethidium bromide by competing for intercalation sites on calf-thymus DNA. Metal complexes having kinetically exchangable ligands, including the antitumor drugs cis-[(NH3)2PtCl2] and [(en)PtC12], inhibit noncompetitively the DNA-associated ethidium fluorescence enhancement by binding covalently to the bases and blocking potential intercalation sites. Only the metallointercalators were capable of altering the DNA duplex winding, as judged by the effects of these reagents upon the electrophoretic mobility and sedimentation behavior of PM-2 DNAs. Long-term (t greater than 120 h) interactions of metal complexes with PM-2 DNAs I, I0, and II, corresponding to superhelical, closed relaxed, and nicked circles, respectively, showed that covalent binding occurs the most readily to DNA I, possibly because of the presence of underwound duplex regions in this tightly wound superhelical DNA. The active antitumor drugs cis-[(NH3)2PtC12] and [(en)PtC12] bind covalently to DNA I under conditions where the inactive trans- [(NH3)2PtC12] does not. Most of the complexes studied were capable of producing chain scissions in PM-2DNA I. Exceptions are the kinetically inert complexes [(bipy)Pt(en)]2+ and (terpy)Pt(SCH2CH2OH)]+, suggesting that covalent binding might be a prerequisite for nicking.", "contents": "Binding of platinum and palladium metallointercalation reagents and antitumor drugs to closed and open DNAs. The interaction of platinum and palladium complexes with closed and nicked circular and linear DNAs was investigated by a variety of methods. Cationic metal complexes containing flat, aromatic ligands, such as 2,2',2''-terpyridine, o-phenanthroline, and 2,2'-bipyridine, interfere with the usual fluorescence enhancement of ethidium bromide by competing for intercalation sites on calf-thymus DNA. Metal complexes having kinetically exchangable ligands, including the antitumor drugs cis-[(NH3)2PtCl2] and [(en)PtC12], inhibit noncompetitively the DNA-associated ethidium fluorescence enhancement by binding covalently to the bases and blocking potential intercalation sites. Only the metallointercalators were capable of altering the DNA duplex winding, as judged by the effects of these reagents upon the electrophoretic mobility and sedimentation behavior of PM-2 DNAs. Long-term (t greater than 120 h) interactions of metal complexes with PM-2 DNAs I, I0, and II, corresponding to superhelical, closed relaxed, and nicked circles, respectively, showed that covalent binding occurs the most readily to DNA I, possibly because of the presence of underwound duplex regions in this tightly wound superhelical DNA. The active antitumor drugs cis-[(NH3)2PtC12] and [(en)PtC12] bind covalently to DNA I under conditions where the inactive trans- [(NH3)2PtC12] does not. Most of the complexes studied were capable of producing chain scissions in PM-2DNA I. Exceptions are the kinetically inert complexes [(bipy)Pt(en)]2+ and (terpy)Pt(SCH2CH2OH)]+, suggesting that covalent binding might be a prerequisite for nicking.", "PMID": 963039}
{"id": "pubmed23n0032_5345", "title": "The effect of hydrogen peroxide on CO2 fixation of isolated intact chloroplasts.", "content": "Low concentration of hydrogen peroxide strongly inhibit CO2 fixation of isolated intact chloroplasts (50% inhibition at 10(-5) M hydrogen peroxide). Addition of catalase to a suspension of intact chloroplasts stimulates CO2 fixation 2--6 fold, indicating that this process is partially inhibited by endogenous hydrogen peroxide formed in a Mehler reaction. The rate of CO2 fixation is strongly increased by addition of Calvin cycle intermediates if the catalase activity of the preparation is low. However, at high catalase activity addition of Calvin cycle intermediates remains without effect. Obviously the hydrogen peroxide formed at low catalase activity leads to a loss of Calvin cycle substrates which reduces the rate of CO2 fixation. 3-Phosphoglycerate-dependent O2-evolution is not influenced by hydrogen peroxide at a concentration (5x10(-4) M) which inhibits CO2 fixation almost completely. Therefore the inhibition site of hydrogen peroxide cannot be at the step of 3-phosphoglycerate reduction. Dark CO2 fixation of lysed chloroplasts in a hypotonic medium is not or only slightly inhibited by hydrogen peroxide (2,5x10(-4) M), if ribulose-1,5-diphosphate, ribose 5-phosphate or xylulose 5-phosphate were added as substrates. However, there is a strong inhibition of CO2 fixation by hydrogen peroxide, if fructose 6-phosphate together with triose phosphate are used as substrates. This indicates that hydrogen peroxide interrupts the Calvin cycle at the transketolase step, leading to a reduced supply of the CO2-acceptor ribulose 1,5-diphosphate.", "contents": "The effect of hydrogen peroxide on CO2 fixation of isolated intact chloroplasts. Low concentration of hydrogen peroxide strongly inhibit CO2 fixation of isolated intact chloroplasts (50% inhibition at 10(-5) M hydrogen peroxide). Addition of catalase to a suspension of intact chloroplasts stimulates CO2 fixation 2--6 fold, indicating that this process is partially inhibited by endogenous hydrogen peroxide formed in a Mehler reaction. The rate of CO2 fixation is strongly increased by addition of Calvin cycle intermediates if the catalase activity of the preparation is low. However, at high catalase activity addition of Calvin cycle intermediates remains without effect. Obviously the hydrogen peroxide formed at low catalase activity leads to a loss of Calvin cycle substrates which reduces the rate of CO2 fixation. 3-Phosphoglycerate-dependent O2-evolution is not influenced by hydrogen peroxide at a concentration (5x10(-4) M) which inhibits CO2 fixation almost completely. Therefore the inhibition site of hydrogen peroxide cannot be at the step of 3-phosphoglycerate reduction. Dark CO2 fixation of lysed chloroplasts in a hypotonic medium is not or only slightly inhibited by hydrogen peroxide (2,5x10(-4) M), if ribulose-1,5-diphosphate, ribose 5-phosphate or xylulose 5-phosphate were added as substrates. However, there is a strong inhibition of CO2 fixation by hydrogen peroxide, if fructose 6-phosphate together with triose phosphate are used as substrates. This indicates that hydrogen peroxide interrupts the Calvin cycle at the transketolase step, leading to a reduced supply of the CO2-acceptor ribulose 1,5-diphosphate.", "PMID": 963040}
{"id": "pubmed23n0032_5346", "title": "Concentration-dependent effects of salicylaldoxime on chloroplast reactions.", "content": "Salicylaldoxime has been found to have a variety of concentration-dependent effects on chloroplast activities. At low concentrations (less than 10 mM), salicyladoxime reversibly inhibits all reactions which involve Photosystem II. Since the DCMU-insensitive silicomolybdate Hill reaction is also inhibited, one site of inhibition is definitely located before the DCMU-sensitive site, possibly before the photoact. The inhibition kinetics and the response of chloroplast fluorescence may indicate another site in the DCMU-sensitive region. At almost exactly the same concentrations (less than 10 mM), salicylaldoxime uncouples phosphorylation reversibly, whether it is supported by Photosystem II or by Photosystem I. At higher concentrations (approx. 20 mM) salicylaldoxime inhibits Photosystem II irreversibly, uncouples irreversibly, and begins to cause changes in chloroplast light scattering which could be manifestations of membrane damage. At very high concentrations (approx. 45 mM) salicylaldoxime irreversibly inhibits Photosystem I activity in the region of plastocyanin. This is indicated by the ability of salicylaldoxime to inhibit the photooxidation of cytochrome f but not the photooxidation of P-700.", "contents": "Concentration-dependent effects of salicylaldoxime on chloroplast reactions. Salicylaldoxime has been found to have a variety of concentration-dependent effects on chloroplast activities. At low concentrations (less than 10 mM), salicyladoxime reversibly inhibits all reactions which involve Photosystem II. Since the DCMU-insensitive silicomolybdate Hill reaction is also inhibited, one site of inhibition is definitely located before the DCMU-sensitive site, possibly before the photoact. The inhibition kinetics and the response of chloroplast fluorescence may indicate another site in the DCMU-sensitive region. At almost exactly the same concentrations (less than 10 mM), salicylaldoxime uncouples phosphorylation reversibly, whether it is supported by Photosystem II or by Photosystem I. At higher concentrations (approx. 20 mM) salicylaldoxime inhibits Photosystem II irreversibly, uncouples irreversibly, and begins to cause changes in chloroplast light scattering which could be manifestations of membrane damage. At very high concentrations (approx. 45 mM) salicylaldoxime irreversibly inhibits Photosystem I activity in the region of plastocyanin. This is indicated by the ability of salicylaldoxime to inhibit the photooxidation of cytochrome f but not the photooxidation of P-700.", "PMID": 963041}
{"id": "pubmed23n0032_5347", "title": "Phosphorylation of nucleotides bound to chloroplast membranes and their role in photophosphorylation.", "content": "1. The initial rapid phosphorylation of membrane-bound ADP yields [gamma- 32P]-ATP. Long term illumination of chloroplasts resulted in the introduction of label in the beta position of ADP and ATP. 2. It is concluded that the initial acceptor in photophosphorylation is ADP and not AMP. The appearance of 32Pi in the beta position of the nucleotide fractions is probably the result of side reactions not directly involved in the photophosphorylation mechanism. 3. The inhibitor phlorizin affects similarly the phosphorylation of bound ADP and the net photophosphorylation reaction. 4. The uncoupler nigericin has different effects on the phosphorylation of membrane-bound ADP and the net photophosphorylation reaction. Dissipation of energy by low concentrations of the uncoupler affects primarily the step(s) of ATP release to the medium, while steps leading to the interconversion of Pi and ADP to form bound ATP are less sensitive and are inhibited by relatively higher uncoupler concentrations.", "contents": "Phosphorylation of nucleotides bound to chloroplast membranes and their role in photophosphorylation. 1. The initial rapid phosphorylation of membrane-bound ADP yields [gamma- 32P]-ATP. Long term illumination of chloroplasts resulted in the introduction of label in the beta position of ADP and ATP. 2. It is concluded that the initial acceptor in photophosphorylation is ADP and not AMP. The appearance of 32Pi in the beta position of the nucleotide fractions is probably the result of side reactions not directly involved in the photophosphorylation mechanism. 3. The inhibitor phlorizin affects similarly the phosphorylation of bound ADP and the net photophosphorylation reaction. 4. The uncoupler nigericin has different effects on the phosphorylation of membrane-bound ADP and the net photophosphorylation reaction. Dissipation of energy by low concentrations of the uncoupler affects primarily the step(s) of ATP release to the medium, while steps leading to the interconversion of Pi and ADP to form bound ATP are less sensitive and are inhibited by relatively higher uncoupler concentrations.", "PMID": 963042}
{"id": "pubmed23n0032_5348", "title": "Anisotropy of photosynthetic membranes and the degree of fluorescence polarization.", "content": "The degree of fluorescence polarization, P, of unoriented and magnetically oriented spinach chloroplasts as a function of excitation (400-680 nm) and emission wavelengths (675-750 nm) is reported. For unoriented chloroplasts P can be divided into two contributions, PIN and PAN. The latter arises from the optical anisotropy of the membranes which is due to the orientation with respect to the membrane plane of pigment molecules in vivo. The intrinsic polarization PIN, which reflects the energy transfer between different pigment molecules and their degree of mutual orientation, can be measured unambiguously only if (1) oriented membranes are used and the fluorescence is viewed along a direction normal to the membrane planes, and (2) the excitation is confined to the Qy (approximately 660-680 nm) absorption band of chlorophyll in vivo. With 670-680 nm excitation, values of P using unoriented chloroplasts can be as high as +14%, mostly reflecting the orientational anisotropy of the pigments. Using oriented chloroplasts PIN is shown to be +5+/-1%. The excitation wavelength dependence studies of PIN indicate that the carotenoid and chlorophyll Qy transition moments tends to be partially oriented with respect to each other on a local level (within a given photosynthetic unit or its immediate neighbors).", "contents": "Anisotropy of photosynthetic membranes and the degree of fluorescence polarization. The degree of fluorescence polarization, P, of unoriented and magnetically oriented spinach chloroplasts as a function of excitation (400-680 nm) and emission wavelengths (675-750 nm) is reported. For unoriented chloroplasts P can be divided into two contributions, PIN and PAN. The latter arises from the optical anisotropy of the membranes which is due to the orientation with respect to the membrane plane of pigment molecules in vivo. The intrinsic polarization PIN, which reflects the energy transfer between different pigment molecules and their degree of mutual orientation, can be measured unambiguously only if (1) oriented membranes are used and the fluorescence is viewed along a direction normal to the membrane planes, and (2) the excitation is confined to the Qy (approximately 660-680 nm) absorption band of chlorophyll in vivo. With 670-680 nm excitation, values of P using unoriented chloroplasts can be as high as +14%, mostly reflecting the orientational anisotropy of the pigments. Using oriented chloroplasts PIN is shown to be +5+/-1%. The excitation wavelength dependence studies of PIN indicate that the carotenoid and chlorophyll Qy transition moments tends to be partially oriented with respect to each other on a local level (within a given photosynthetic unit or its immediate neighbors).", "PMID": 963043}
{"id": "pubmed23n0032_5349", "title": "Kinetics and stoichiometry of light-induced proton release and uptake from purple membrane fragments, Halobacterium halobium cell envelopes, and phospholipid vesicles containing oriented purple membrane.", "content": "We have used flash spectroscopy and pH indicator dyes to measure the kinetics and stoichiometry of light-induced proton release and uptake by purple membrane in aqueous suspension, in cell envelope vesicles and in lipid vesicles. The preferential orientation of bacteriorhodopsin in opposite directions in the envelope and lipid vesicles allows us to show that uptake of protons occurs on the cytoplasmic side of the purple membrane and release on the exterior side. In suspensions of isolated purple membrane, approximately one proton per cycling bacteriorhodopsin molecule appears transiently in the aqueous phase with a half-rise time of 0.8 ms and a half-decay time of 5.4 ms at 21degreesC. In cell envelope preparations which consist of vesicles with a preferential orientation of purple membrane, as in whole cells, and which pump protons out, the acidification of the medium has a half-rise time of less than 1.0 ms, which partially relaxes in approx. 10 ms and fully relaxes after many seconds. Phospholipid vesicles, which contain bacteriorhodopsin preferentially oriented in the opposite direction and pump protons in, show an alkalinization of the medium with a time constant of approximately 10 ms, preceded by a much smaller and faster acidification. The alkalinization relaxes over many seconds. The initial fast acidification in the lipid vesicles and the fast relaxation in the envelope vesicles are accounted for by the misoriented fractions of bacteriorhodopsin. The time constants of the main effects, acidification in the envelopes and alkalinization in the lipid vesicles correlate with the time constants for the release and uptake of protons in the isolated purple membrane, and therefore show that these must occur on the outer and inner surface respectively. The slow relaxation processes in the time range of several seconds must be attributed to the passive back diffusion of protons through the vesicle membrane.", "contents": "Kinetics and stoichiometry of light-induced proton release and uptake from purple membrane fragments, Halobacterium halobium cell envelopes, and phospholipid vesicles containing oriented purple membrane. We have used flash spectroscopy and pH indicator dyes to measure the kinetics and stoichiometry of light-induced proton release and uptake by purple membrane in aqueous suspension, in cell envelope vesicles and in lipid vesicles. The preferential orientation of bacteriorhodopsin in opposite directions in the envelope and lipid vesicles allows us to show that uptake of protons occurs on the cytoplasmic side of the purple membrane and release on the exterior side. In suspensions of isolated purple membrane, approximately one proton per cycling bacteriorhodopsin molecule appears transiently in the aqueous phase with a half-rise time of 0.8 ms and a half-decay time of 5.4 ms at 21degreesC. In cell envelope preparations which consist of vesicles with a preferential orientation of purple membrane, as in whole cells, and which pump protons out, the acidification of the medium has a half-rise time of less than 1.0 ms, which partially relaxes in approx. 10 ms and fully relaxes after many seconds. Phospholipid vesicles, which contain bacteriorhodopsin preferentially oriented in the opposite direction and pump protons in, show an alkalinization of the medium with a time constant of approximately 10 ms, preceded by a much smaller and faster acidification. The alkalinization relaxes over many seconds. The initial fast acidification in the lipid vesicles and the fast relaxation in the envelope vesicles are accounted for by the misoriented fractions of bacteriorhodopsin. The time constants of the main effects, acidification in the envelopes and alkalinization in the lipid vesicles correlate with the time constants for the release and uptake of protons in the isolated purple membrane, and therefore show that these must occur on the outer and inner surface respectively. The slow relaxation processes in the time range of several seconds must be attributed to the passive back diffusion of protons through the vesicle membrane.", "PMID": 963044}
{"id": "pubmed23n0032_5350", "title": "Light-dependent inhibitory action of p-nitrothiophenol on photosystem II in relation to the redox state of electron carriers.", "content": "The photosystem-II activity of chloroplasts was inhibited by the treatment with p-nitrothiophenol (NphSH) in the light, and the inhibition was accompanied by a change of the fluorescence spectrum. Aromatic mercaptans examined were active in causing this inhibition and fluorescence change. These effects of p-nitrothiophenol were highly accelerated by blocking the electron transport of the oxidation side of photosystem II by carbonyl-cyanide-m-chlorophenylhydrazone (CCCP) or Tris-HC1 or heat pre-treatment, whereas these were suppressed by blocking the transport on the reduction side by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). It was deduced that the site of NphSH action in the electron transport chain is closer to the reaction center of photosystem II that the blocking site of CCCP or Tris-HC1 or heat, and that such a site in photosystem II is exposed to be modified with NphSH when electron carriers on the oxidation side of photosystem II are oxidized by illumination.", "contents": "Light-dependent inhibitory action of p-nitrothiophenol on photosystem II in relation to the redox state of electron carriers. The photosystem-II activity of chloroplasts was inhibited by the treatment with p-nitrothiophenol (NphSH) in the light, and the inhibition was accompanied by a change of the fluorescence spectrum. Aromatic mercaptans examined were active in causing this inhibition and fluorescence change. These effects of p-nitrothiophenol were highly accelerated by blocking the electron transport of the oxidation side of photosystem II by carbonyl-cyanide-m-chlorophenylhydrazone (CCCP) or Tris-HC1 or heat pre-treatment, whereas these were suppressed by blocking the transport on the reduction side by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). It was deduced that the site of NphSH action in the electron transport chain is closer to the reaction center of photosystem II that the blocking site of CCCP or Tris-HC1 or heat, and that such a site in photosystem II is exposed to be modified with NphSH when electron carriers on the oxidation side of photosystem II are oxidized by illumination.", "PMID": 963045}
{"id": "pubmed23n0032_5351", "title": "A highactivity ATP translocator in mesophyll chloroplasts of Digitaria sanguinalis, a plant having the C-4 dicarboxylic acid pathway of photosynthesis.", "content": "The effect of exogenous adenine nucleotides on CO2 fixation and oxygen evolution was studied with mesophyll protoplast extracts of the C4 plant Digitaria sanguinalis. Exogenous ATP was found to stimulate the rate of pyruvate and pyruvate+oxalacetate induced CO2 fixation, as well as reverse the inhibition of CO2 fixation of carbonyl cyanide m-chlorophenyl hydrazone and several electron transport inhibitors. The ATP-dependent stimulation of CO2 fixation varied from 40 to 70 mumol CO2 fixed/mg chlorophyll per h, suggesting that ATP was crossing the chloroplast membranes at rates of 80-140 mumol/mg chlorophyll per h, since 2 ATP are required for each CO2 fixed. Fixation of CO2 could also be induced in the dark by exogenous ATP, in which case ADP accumulated outside the chloroplasts...", "contents": "A highactivity ATP translocator in mesophyll chloroplasts of Digitaria sanguinalis, a plant having the C-4 dicarboxylic acid pathway of photosynthesis. The effect of exogenous adenine nucleotides on CO2 fixation and oxygen evolution was studied with mesophyll protoplast extracts of the C4 plant Digitaria sanguinalis. Exogenous ATP was found to stimulate the rate of pyruvate and pyruvate+oxalacetate induced CO2 fixation, as well as reverse the inhibition of CO2 fixation of carbonyl cyanide m-chlorophenyl hydrazone and several electron transport inhibitors. The ATP-dependent stimulation of CO2 fixation varied from 40 to 70 mumol CO2 fixed/mg chlorophyll per h, suggesting that ATP was crossing the chloroplast membranes at rates of 80-140 mumol/mg chlorophyll per h, since 2 ATP are required for each CO2 fixed. Fixation of CO2 could also be induced in the dark by exogenous ATP, in which case ADP accumulated outside the chloroplasts...", "PMID": 963046}
{"id": "pubmed23n0032_5352", "title": "Binding of radioactively labeled carboxyatractyloside, atractyloside and bongkrekic acid to the ADP translocator of potato mitochondria.", "content": "1. The inhibition of the ADP-stimulated respiration of potato mitochondria by carboxyatractyloside is relieved by high concentration of ADP or by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Atractyloside is a much less potent inhibitor than carboxyatractyloside. The inhibition of the ADP-stimulated respiration required about 60-times more atractyloside than carboxyatractyloside. 2. [35S]carboxyatractyloside and [3H]bongkrekic acid bind to potato mitochondria with high affinity (Kd = 10 to 20 nM, n=0.6-0.7 nmol per mg protein). Added ADP competes with carboxyatractyloside for binding; on the contrary ADP increases the amount of bound bongkrekic acid. [3H]atractyloside binds to potato mitochondria with a much lower affinity (Kd=0.45 muM) than carboxyatractyloside or bongkrekic acid. 3. Bound [3H]atractyloside is displaced by ADP, carboxyatractyloside and bongkrekic acid. The displacement of bound [35S]carboxyatractyloside by bongkrekic acid and of bound [3H]bongkrekic acid by carboxyatractyloside is markedly increased by ADP. 4. Bongkrekic acid competes with [35S]carboxyatractyloside for binding. Addition of a small concentration of ADP considerably enhances the inhibitory effect of bongkrekic acid on [35S]carboxyatractyloside binding. 5. The adenine nucleotide content of potato mitochondria is of the order of 1 nmol per mg protein. ADP transport in potato mitochondria is inhibited by atractyloside 30- to 40-times less efficiently than by carboxyatractyloside.", "contents": "Binding of radioactively labeled carboxyatractyloside, atractyloside and bongkrekic acid to the ADP translocator of potato mitochondria. 1. The inhibition of the ADP-stimulated respiration of potato mitochondria by carboxyatractyloside is relieved by high concentration of ADP or by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Atractyloside is a much less potent inhibitor than carboxyatractyloside. The inhibition of the ADP-stimulated respiration required about 60-times more atractyloside than carboxyatractyloside. 2. [35S]carboxyatractyloside and [3H]bongkrekic acid bind to potato mitochondria with high affinity (Kd = 10 to 20 nM, n=0.6-0.7 nmol per mg protein). Added ADP competes with carboxyatractyloside for binding; on the contrary ADP increases the amount of bound bongkrekic acid. [3H]atractyloside binds to potato mitochondria with a much lower affinity (Kd=0.45 muM) than carboxyatractyloside or bongkrekic acid. 3. Bound [3H]atractyloside is displaced by ADP, carboxyatractyloside and bongkrekic acid. The displacement of bound [35S]carboxyatractyloside by bongkrekic acid and of bound [3H]bongkrekic acid by carboxyatractyloside is markedly increased by ADP. 4. Bongkrekic acid competes with [35S]carboxyatractyloside for binding. Addition of a small concentration of ADP considerably enhances the inhibitory effect of bongkrekic acid on [35S]carboxyatractyloside binding. 5. The adenine nucleotide content of potato mitochondria is of the order of 1 nmol per mg protein. ADP transport in potato mitochondria is inhibited by atractyloside 30- to 40-times less efficiently than by carboxyatractyloside.", "PMID": 963047}
{"id": "pubmed23n0032_5353", "title": "Deuterium oxide effects on excitation-contraction coupling of skeletal muscle.", "content": "2H2O (99.8%) Ringer's solution greatly reduces the twitch and tetanus of frog sartorius muscle and, as specially shown here, slows the onset features of the mechanical output of the twitch by: (a) increasing the time (LR) from stimulus to start of latency relaxation; (b) slowing the development of the latency relaxation, and (c) greatly decreasing the rate of onset of tension development. These changes reflect effects of 2H2O on excitation-contraction coupling and they represent the critical direct effects of 2H2O on muscle since it does not depress either the action potential or the intrinsic myofibrillar contractility. The increase in LR is attributed to slowed inward electrical propagation in the T-tubule. But the critical effect of 2H2O on frog muscle is to greatly depress mobilization of activator Ca2+. The depression of the Ca2+ mobilization and of its effects on the activation of contraction evidently result from (a) a lowered rate of release of Ca2+ from the sar coplasmic reticulum, as indicated by the slowed development of the latency relaxation, (b) a decreased amount of Ca2+ released in a twitch, and (c) a reduced speed of diffusion of the Ca2+ to the contractile filaments. The depressed mobilization of Ca2+ is apparently the essential  cause of 2H2O's general depression of twitch and tetanus output.", "contents": "Deuterium oxide effects on excitation-contraction coupling of skeletal muscle. 2H2O (99.8%) Ringer's solution greatly reduces the twitch and tetanus of frog sartorius muscle and, as specially shown here, slows the onset features of the mechanical output of the twitch by: (a) increasing the time (LR) from stimulus to start of latency relaxation; (b) slowing the development of the latency relaxation, and (c) greatly decreasing the rate of onset of tension development. These changes reflect effects of 2H2O on excitation-contraction coupling and they represent the critical direct effects of 2H2O on muscle since it does not depress either the action potential or the intrinsic myofibrillar contractility. The increase in LR is attributed to slowed inward electrical propagation in the T-tubule. But the critical effect of 2H2O on frog muscle is to greatly depress mobilization of activator Ca2+. The depression of the Ca2+ mobilization and of its effects on the activation of contraction evidently result from (a) a lowered rate of release of Ca2+ from the sar coplasmic reticulum, as indicated by the slowed development of the latency relaxation, (b) a decreased amount of Ca2+ released in a twitch, and (c) a reduced speed of diffusion of the Ca2+ to the contractile filaments. The depressed mobilization of Ca2+ is apparently the essential  cause of 2H2O's general depression of twitch and tetanus output.", "PMID": 963048}
{"id": "pubmed23n0032_5354", "title": "Multiple-flash development of thermoluminescence bands in dark-grown spruce leaves.", "content": "Spruce leaves greened in darkness were devoid of three of the five thermoluminescence bands found for mature leaves. These bands were developed rapidly by exposure of the dark-grown leaves to continuous light. The development of these bands was studied by illumination with repetitive flashes at varied intervals. Flashes at intervals of 1 s were the most effective in inducing these bands. Those at shorter or longer intervals were less effective. It was deduced from these data that the development of these bands is a multiquantum process which involves at least two photo-events with a dark reaction between them.", "contents": "Multiple-flash development of thermoluminescence bands in dark-grown spruce leaves. Spruce leaves greened in darkness were devoid of three of the five thermoluminescence bands found for mature leaves. These bands were developed rapidly by exposure of the dark-grown leaves to continuous light. The development of these bands was studied by illumination with repetitive flashes at varied intervals. Flashes at intervals of 1 s were the most effective in inducing these bands. Those at shorter or longer intervals were less effective. It was deduced from these data that the development of these bands is a multiquantum process which involves at least two photo-events with a dark reaction between them.", "PMID": 963049}
{"id": "pubmed23n0032_5355", "title": "Maturation of ribosomes in yeast. I Kinetic analysis by labelling of high molecular weight rRNA species.", "content": "To study the maturation of ribosomes in Saccharomyces carlsbergensis, protoplasts were pulse labeled with [5-3H]uridine at 15 degrees C. Investigation of the cellular location of pulse-labelled ribosomal RNA precursor and mature ribosomal RNA shows that both the 37-S precursor RNA, common to both 17-S and 26-S rRNA, as well as the 29-S RNA, the direct precursor of 26-S rRNA, are located in the nucleus. Most of the 18-S RNA, the direct precursor of 17-S rRNA, is found in the cytoplasmic fraction. Apart from 37-S and 29-S RNA the nucleus also contains an appreciable amount of 26-S rRNA as well as a small quantity of 18-S RNA. These data indicate that processing of 29-S to 26-S RNA occurs in the nucleus, whereas the conversion of 18-S RNA to 17-S rRNA takes place in the cytoplasm. The kinetics of appearance of pulse-labelled 26-S and 17-S rRNA in the various cytoplasmic ribosomal particles indicate, that newly formed 40-S ribosomal particles are almost immediately incorporated into 80-S ribosomes and polysomes. On the other hand, there appears to exist a fairly large cytoplasmic pool of newly synthesized ribosomal particles containing 26-S rRNA and sedimenting at about 60 S. The kinetics of appearance of newly formed 26-S and 17-S rRNA in mature ribosomes show that the maturation of the large ribosomal subunit takes about twice as much time as that of the small subunit.", "contents": "Maturation of ribosomes in yeast. I Kinetic analysis by labelling of high molecular weight rRNA species. To study the maturation of ribosomes in Saccharomyces carlsbergensis, protoplasts were pulse labeled with [5-3H]uridine at 15 degrees C. Investigation of the cellular location of pulse-labelled ribosomal RNA precursor and mature ribosomal RNA shows that both the 37-S precursor RNA, common to both 17-S and 26-S rRNA, as well as the 29-S RNA, the direct precursor of 26-S rRNA, are located in the nucleus. Most of the 18-S RNA, the direct precursor of 17-S rRNA, is found in the cytoplasmic fraction. Apart from 37-S and 29-S RNA the nucleus also contains an appreciable amount of 26-S rRNA as well as a small quantity of 18-S RNA. These data indicate that processing of 29-S to 26-S RNA occurs in the nucleus, whereas the conversion of 18-S RNA to 17-S rRNA takes place in the cytoplasm. The kinetics of appearance of pulse-labelled 26-S and 17-S rRNA in the various cytoplasmic ribosomal particles indicate, that newly formed 40-S ribosomal particles are almost immediately incorporated into 80-S ribosomes and polysomes. On the other hand, there appears to exist a fairly large cytoplasmic pool of newly synthesized ribosomal particles containing 26-S rRNA and sedimenting at about 60 S. The kinetics of appearance of newly formed 26-S and 17-S rRNA in mature ribosomes show that the maturation of the large ribosomal subunit takes about twice as much time as that of the small subunit.", "PMID": 963050}
{"id": "pubmed23n0032_5356", "title": "Maturation of ribosomes in yeast. II. Position of the low molecular weight rRNA species in the maturation process.", "content": "Yeast protoplasts were pulse labelled with [5-3H] uridine and the labelling kinetics of the low molecular weight rRNA species were determined in order to gain more insight into the position of those small rRNAs in the process of ribosome maturation. 7-S RNA, the immediate precursor of 5.8-S rRNA, is found to be present only in the nucleus, indicating that the conversion of 7-S to 5.8-S RNA is a nuclear event. 5.8-S rRNA is observed in the cytoplasm almost immediately after its formation. This as well as the presence of only a small amount of 5.8-S RNA in the nucleus, shows that the ribosomal precursor particles of the large ribosomal subunit are very rapidly transported into the cytoplasm once 5.8-S rRNA is formed. Most of the newly synthesized 5-S RNA is found in the nucleus. This nuclear 5-S rRNA is mainly present in the ribosomal precursor particles. However, a small pool of free 5-S rRNA is probably also present.", "contents": "Maturation of ribosomes in yeast. II. Position of the low molecular weight rRNA species in the maturation process. Yeast protoplasts were pulse labelled with [5-3H] uridine and the labelling kinetics of the low molecular weight rRNA species were determined in order to gain more insight into the position of those small rRNAs in the process of ribosome maturation. 7-S RNA, the immediate precursor of 5.8-S rRNA, is found to be present only in the nucleus, indicating that the conversion of 7-S to 5.8-S RNA is a nuclear event. 5.8-S rRNA is observed in the cytoplasm almost immediately after its formation. This as well as the presence of only a small amount of 5.8-S RNA in the nucleus, shows that the ribosomal precursor particles of the large ribosomal subunit are very rapidly transported into the cytoplasm once 5.8-S rRNA is formed. Most of the newly synthesized 5-S RNA is found in the nucleus. This nuclear 5-S rRNA is mainly present in the ribosomal precursor particles. However, a small pool of free 5-S rRNA is probably also present.", "PMID": 963051}
{"id": "pubmed23n0032_5357", "title": "Evidence for the synthesis of tubulin on membrane-bound and free ribosomes from rat forebrain.", "content": "Evidence is presented that tubulin is synthesized in cell-free systems derived from rat forebrain containing either membrane-bound or free ribosomes. Both systems were supplemented with saturating amounts of messenger RNA (from either membrane-bound of free ribosomes) and soluble enzyme and initiation factors from whole forebrain. Synthesized products were chracterized by two biochemical purification methods for brain tubulin isolation. These methods of tubulin analysis indicated that both cell free systems synthesized the same relative amounts of tubulin.", "contents": "Evidence for the synthesis of tubulin on membrane-bound and free ribosomes from rat forebrain. Evidence is presented that tubulin is synthesized in cell-free systems derived from rat forebrain containing either membrane-bound or free ribosomes. Both systems were supplemented with saturating amounts of messenger RNA (from either membrane-bound of free ribosomes) and soluble enzyme and initiation factors from whole forebrain. Synthesized products were chracterized by two biochemical purification methods for brain tubulin isolation. These methods of tubulin analysis indicated that both cell free systems synthesized the same relative amounts of tubulin.", "PMID": 963052}
{"id": "pubmed23n0032_5358", "title": "Effects of ionizing radiation of RNA metabolism in cultured mammalian cells. III. Specific reduction in mRNA synthesis during resumed division and plateau phases following exposure to 800 rads of x irradiation.", "content": "The cell growth response of cultured Chinese hamster ovary cells, line CHO, to 800 rads of X irradiation involves a period of division delay, followed by a period of resumed division which terminates in a plateau phase. Over 95% of the cells die eventually. No direct effects of RNA or protein metabolism are evident during the delay period. During the resumed division and beginning of plateau phases, however, a specific and relatively constant reduction in mRNA synthesis relative to messenger-like RNA and heterogenous nuclear RNA synthesis is evidenced. The ratio of mRNA to messenger-like RNA synthesis ranges from 0.8 to 0.65 during these phases. The effect is not due to altered cell-cycle distribution, and evidence is presented to indicate that it is probably not a compensatory response to the unbalanced growth that occurs during the division delay period.", "contents": "Effects of ionizing radiation of RNA metabolism in cultured mammalian cells. III. Specific reduction in mRNA synthesis during resumed division and plateau phases following exposure to 800 rads of x irradiation. The cell growth response of cultured Chinese hamster ovary cells, line CHO, to 800 rads of X irradiation involves a period of division delay, followed by a period of resumed division which terminates in a plateau phase. Over 95% of the cells die eventually. No direct effects of RNA or protein metabolism are evident during the delay period. During the resumed division and beginning of plateau phases, however, a specific and relatively constant reduction in mRNA synthesis relative to messenger-like RNA and heterogenous nuclear RNA synthesis is evidenced. The ratio of mRNA to messenger-like RNA synthesis ranges from 0.8 to 0.65 during these phases. The effect is not due to altered cell-cycle distribution, and evidence is presented to indicate that it is probably not a compensatory response to the unbalanced growth that occurs during the division delay period.", "PMID": 963053}
{"id": "pubmed23n0032_5359", "title": "Culture conditions affect photoreactivating enzyme levels in human fibroblasts.", "content": "Photoreactivation of pyrimidine dimers in mammalian cells occurs under our experimental conditions but has not been observed under conditions used by others. We have tested three possible differences in experimental procedures including dimer separation and analysis methods, illumination conditions and cell culture techniques. We show that out methods of dimer separation and analysis indeed measure cis-syn pyrimidine dimers and give results in quantitative agreement with the methods of others. We find that while light pre-illumination of fibroblasts from the xeroderma pigmentosum line XP12BE or of normal cells does not affect the cellular capacity for dimer photoreactivation. However, we show that cell culture conditions can affect photoreactivating enzyme levels and thus, cellular dimer photoreactivation capacity. Cells grown in Eagle's minimal essential medium (supplemented with 15% fetal bovine serum) contain very low levels of photoreactivating enzyme and cannot photoreactivate dimers in their DNA; however, companion cultures maintained in Dulbecco's modified Eagle's minimal medium do contain photoreactivating enzyme and can photoreactivate cellular dimers.", "contents": "Culture conditions affect photoreactivating enzyme levels in human fibroblasts. Photoreactivation of pyrimidine dimers in mammalian cells occurs under our experimental conditions but has not been observed under conditions used by others. We have tested three possible differences in experimental procedures including dimer separation and analysis methods, illumination conditions and cell culture techniques. We show that out methods of dimer separation and analysis indeed measure cis-syn pyrimidine dimers and give results in quantitative agreement with the methods of others. We find that while light pre-illumination of fibroblasts from the xeroderma pigmentosum line XP12BE or of normal cells does not affect the cellular capacity for dimer photoreactivation. However, we show that cell culture conditions can affect photoreactivating enzyme levels and thus, cellular dimer photoreactivation capacity. Cells grown in Eagle's minimal essential medium (supplemented with 15% fetal bovine serum) contain very low levels of photoreactivating enzyme and cannot photoreactivate dimers in their DNA; however, companion cultures maintained in Dulbecco's modified Eagle's minimal medium do contain photoreactivating enzyme and can photoreactivate cellular dimers.", "PMID": 963054}
{"id": "pubmed23n0032_5360", "title": "Partial purification and properties of the reticulocyte guanylating enzyme.", "content": "The guanylating enzyme which catalyzes the insertion of a guanine residue into one of the isoacceping tRNAHis of rabbit reticulocytes has been purified approximately one-hundred fold. It is free of nuclease activity. The enzyme does not catalyze the replacement of inserted radioactive guanine by unlabeled guanine, indicating that the reaction is irreversible. We have separated the histidyl-tRNA of reticulocytes into three isoacceptors. Previous work showed that the last histidyl-tRNA to elute from RPC-5 columns was the product of the guanylation reaction. This reports shows that the same late-eluting peak also contains the substrate for the guanylating enzyme, indicating that the guanine insertion reaction is chromatographically silent. The isoaccepting tRNAHis that is the substrate for the guanylating enzyme does not contain the hypermodified base known as Q. It is the other major reticulocyte tRNAHis that coantains Q, showing that at least in the reticulocyte the role of the guanylating enzyme is not the conversion of the Q form of tRNA to the homogeneic G form. The purified enzyme does not insert any base other than guanine into tRNA.", "contents": "Partial purification and properties of the reticulocyte guanylating enzyme. The guanylating enzyme which catalyzes the insertion of a guanine residue into one of the isoacceping tRNAHis of rabbit reticulocytes has been purified approximately one-hundred fold. It is free of nuclease activity. The enzyme does not catalyze the replacement of inserted radioactive guanine by unlabeled guanine, indicating that the reaction is irreversible. We have separated the histidyl-tRNA of reticulocytes into three isoacceptors. Previous work showed that the last histidyl-tRNA to elute from RPC-5 columns was the product of the guanylation reaction. This reports shows that the same late-eluting peak also contains the substrate for the guanylating enzyme, indicating that the guanine insertion reaction is chromatographically silent. The isoaccepting tRNAHis that is the substrate for the guanylating enzyme does not contain the hypermodified base known as Q. It is the other major reticulocyte tRNAHis that coantains Q, showing that at least in the reticulocyte the role of the guanylating enzyme is not the conversion of the Q form of tRNA to the homogeneic G form. The purified enzyme does not insert any base other than guanine into tRNA.", "PMID": 963055}
{"id": "pubmed23n0032_5361", "title": "Incorporation of radioactive amino acids into protein in isolated rat hepatocytes.", "content": "The incorporation of radioactivity from a 14C-labelled amino acid mixture (algal protein hydrolysate) into protein in isolated rat hepatocytes has been studied. The incorporation rate declined with increasing cell concentration, an effect which could be explained partly by isotope consumption, partly (and largely) by isotope dilution due to the formation of non-labelled amino acids by the cells. At a high extracellular amino acid concentration, the rate of incorporation into protein became independent of cell concentration, because the isotope dilution effect was now quantitatively insignificant. The time course of protein labelling at various cells concentrations correlated better with the intracellular than with the extracellular amino acid specific activity, suggesting that amino acids for protein synthesis were taken from an intracellular pool. With increasing extracellular amino acid concentrations both the intracellular amino acid concentration, the intracellular radioactivity and the rate of incorporation into protein increased. Protein labelling exhibited a distinct time lag at high amino acid concentrations, presumably reflecting the time-dependent expansion of the intracellular amino acid pool. The gradual increase in the rate of protein labelling could be due either to an increased intracellular specific activity, or to a real stimulation of protein synthesis by amino acids, depending on whether the total intracellular amino acid pool or just the expandable compartment is the precursor pool for protein synthesis.", "contents": "Incorporation of radioactive amino acids into protein in isolated rat hepatocytes. The incorporation of radioactivity from a 14C-labelled amino acid mixture (algal protein hydrolysate) into protein in isolated rat hepatocytes has been studied. The incorporation rate declined with increasing cell concentration, an effect which could be explained partly by isotope consumption, partly (and largely) by isotope dilution due to the formation of non-labelled amino acids by the cells. At a high extracellular amino acid concentration, the rate of incorporation into protein became independent of cell concentration, because the isotope dilution effect was now quantitatively insignificant. The time course of protein labelling at various cells concentrations correlated better with the intracellular than with the extracellular amino acid specific activity, suggesting that amino acids for protein synthesis were taken from an intracellular pool. With increasing extracellular amino acid concentrations both the intracellular amino acid concentration, the intracellular radioactivity and the rate of incorporation into protein increased. Protein labelling exhibited a distinct time lag at high amino acid concentrations, presumably reflecting the time-dependent expansion of the intracellular amino acid pool. The gradual increase in the rate of protein labelling could be due either to an increased intracellular specific activity, or to a real stimulation of protein synthesis by amino acids, depending on whether the total intracellular amino acid pool or just the expandable compartment is the precursor pool for protein synthesis.", "PMID": 963056}
{"id": "pubmed23n0032_5362", "title": "Arterial elastin synthesis in the young chick.", "content": "In the growing chick a marked stimulation in soluble and mature arterial elastin synthesis occurs 2 and 5 weeks after hatching. Measurement of [3H]valine and [3H]proline incorporation into arterial soluble protein during this period indicated that most of the label is found in a 70 000 dalton protein subunit. The labeled soluble subunit had the characteristics of native soluble elastin or tropoelastin. During the period in which the greatest percentage increase in mature elastin occurs, the highest specific activities of soluble [3H]valine-labeled protein were observed. These changes were striking and suggest a developmental period for the growing chick in which factors related to elastin metabolism may be more easily studied.", "contents": "Arterial elastin synthesis in the young chick. In the growing chick a marked stimulation in soluble and mature arterial elastin synthesis occurs 2 and 5 weeks after hatching. Measurement of [3H]valine and [3H]proline incorporation into arterial soluble protein during this period indicated that most of the label is found in a 70 000 dalton protein subunit. The labeled soluble subunit had the characteristics of native soluble elastin or tropoelastin. During the period in which the greatest percentage increase in mature elastin occurs, the highest specific activities of soluble [3H]valine-labeled protein were observed. These changes were striking and suggest a developmental period for the growing chick in which factors related to elastin metabolism may be more easily studied.", "PMID": 963057}
{"id": "pubmed23n0032_5363", "title": "Stimulation of albumin synthesis by keto analogues of amino acids.", "content": "(1) The effect of ketoanalogues of branched-chain amino acids on albumin synthesis was examined in two biological systems using the [14C]carbonate technique. (2)alpha-Ketocaproic acid, the ketoanalogue of leucine, was able to reverse the reduced synthesis rate observed when isolated livers, from well-nourished animals were perfused with blood from rats deprived of dietary protein for 48 h. (3) A mixture of ketoanalogues of the three branched-chain amino acids, leucine, isoleucine and valine, was able to increase albumin synthesis per unit dry liver weight to above normal levels when administered intragastrically to rats 16 h after partial hepatectomy.", "contents": "Stimulation of albumin synthesis by keto analogues of amino acids. (1) The effect of ketoanalogues of branched-chain amino acids on albumin synthesis was examined in two biological systems using the [14C]carbonate technique. (2)alpha-Ketocaproic acid, the ketoanalogue of leucine, was able to reverse the reduced synthesis rate observed when isolated livers, from well-nourished animals were perfused with blood from rats deprived of dietary protein for 48 h. (3) A mixture of ketoanalogues of the three branched-chain amino acids, leucine, isoleucine and valine, was able to increase albumin synthesis per unit dry liver weight to above normal levels when administered intragastrically to rats 16 h after partial hepatectomy.", "PMID": 963058}
{"id": "pubmed23n0032_5364", "title": "The formation and annealing of structural defects in lipid bilayer vesicles.", "content": "It is shown that sonication of phospholipid-water dispersions below the crystalline leads to liquid crystalline phase transition temperature (Tc) produces bilayer vesicles with structural defects within the bilayer membrane, which permit rapid permeation of ions and catalyze vesicle-vesicle fusion. These structural defects are annihilated simply by annealing the vesicle suspension above Tc. The rate of annealing was found to be slow, of the order of an hour for T = 3 degrees C above Tc, but annealing is complete within 10 min for T = 10 degrees C above Tc. It is proposed that these structural defects are fault-dislocations in the bilayer structure, which arise from a population defect in the distribution of the lipid molecules between the outer and inner monolayers, when small bilayer fragments reassemble to form the small bilayer vesicles during the sonication procedure. Such a population defect can only be remedied by lipid transport via the inside in equilibrium outside flip-flop mechanism, which would account for the slow kinetics of annealing observed even at 3 degrees C above the phase transition.", "contents": "The formation and annealing of structural defects in lipid bilayer vesicles. It is shown that sonication of phospholipid-water dispersions below the crystalline leads to liquid crystalline phase transition temperature (Tc) produces bilayer vesicles with structural defects within the bilayer membrane, which permit rapid permeation of ions and catalyze vesicle-vesicle fusion. These structural defects are annihilated simply by annealing the vesicle suspension above Tc. The rate of annealing was found to be slow, of the order of an hour for T = 3 degrees C above Tc, but annealing is complete within 10 min for T = 10 degrees C above Tc. It is proposed that these structural defects are fault-dislocations in the bilayer structure, which arise from a population defect in the distribution of the lipid molecules between the outer and inner monolayers, when small bilayer fragments reassemble to form the small bilayer vesicles during the sonication procedure. Such a population defect can only be remedied by lipid transport via the inside in equilibrium outside flip-flop mechanism, which would account for the slow kinetics of annealing observed even at 3 degrees C above the phase transition.", "PMID": 963059}
{"id": "pubmed23n0032_5365", "title": "Standing-gradient osmotic flow. Examination of its validity using an analytical method.", "content": "The solutions to the non-linear differential equations governing solute-solvent coupling in the intercellular spaces of epithelial layers have been obtained by using an analytical method, rather than the usual numerical ones. When the present series solution includes second-order correction terms, the concentration and velocity profiles obtained by the analytical method agree very well with those coming from numerical solutions. This method has further allowed us to examine the standing-gradient hypothesis when applied to the backwards fluid transport system of the corneal endothelium. With the information presently available for the relevant parameters (osmotic permeability, rate of transport, radius and length of the spaces, and location of the pumping sites), near-isotonicity of the transported fluid would not be explained by the standing-gradient model.", "contents": "Standing-gradient osmotic flow. Examination of its validity using an analytical method. The solutions to the non-linear differential equations governing solute-solvent coupling in the intercellular spaces of epithelial layers have been obtained by using an analytical method, rather than the usual numerical ones. When the present series solution includes second-order correction terms, the concentration and velocity profiles obtained by the analytical method agree very well with those coming from numerical solutions. This method has further allowed us to examine the standing-gradient hypothesis when applied to the backwards fluid transport system of the corneal endothelium. With the information presently available for the relevant parameters (osmotic permeability, rate of transport, radius and length of the spaces, and location of the pumping sites), near-isotonicity of the transported fluid would not be explained by the standing-gradient model.", "PMID": 963060}
{"id": "pubmed23n0032_5366", "title": "Biochemical studies on abnormal erythrocyte membranes. Protein abnormality of erythrocyte membrane in biliary obstruction.", "content": "Biochemical studies on erythrocyte membranes from eleven obstructive jaundice patients (due to various disorders) have been undertaken, By scanning electron microscopic observation these erythrocytes were spur and target in appearance. The lipid composition showed a marked increase in both cholesterol and phosphatidylcholine. In addition to these changes, it was unexpectedly demonstrated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate that a specific membrane protein component 4.2 was reduced or absent in all cases tested. This membrane protein abnormality was identical with that of hereditary spherocytosis erythrocyte membranes. It is of particular interest to note that after surgical relief of biliary obstruction in a typical case of common duct cholelithiasis, the disc electrophoretic pattern of erythrocyte membranes became normal and both lipid composition and red cell morphology returned to normal.", "contents": "Biochemical studies on abnormal erythrocyte membranes. Protein abnormality of erythrocyte membrane in biliary obstruction. Biochemical studies on erythrocyte membranes from eleven obstructive jaundice patients (due to various disorders) have been undertaken, By scanning electron microscopic observation these erythrocytes were spur and target in appearance. The lipid composition showed a marked increase in both cholesterol and phosphatidylcholine. In addition to these changes, it was unexpectedly demonstrated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate that a specific membrane protein component 4.2 was reduced or absent in all cases tested. This membrane protein abnormality was identical with that of hereditary spherocytosis erythrocyte membranes. It is of particular interest to note that after surgical relief of biliary obstruction in a typical case of common duct cholelithiasis, the disc electrophoretic pattern of erythrocyte membranes became normal and both lipid composition and red cell morphology returned to normal.", "PMID": 963061}
{"id": "pubmed23n0032_5367", "title": "Alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes.", "content": "Phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosaminital after treatment with alkaline borohydride. Cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. After gel filtration, a tetrasaccharide was isolated from horse and sheep glycoproteins containing the disaccharide plus two molecules of sialic acid. Periodate oxidation together with paper chromatography of alkaline degraded fragments showed these two molecules of sialic acid to be linked to positions C3 and C6 of the galactosyl and N-acetylgalactosamine residues respectively. Evidence was obtained for a similar structure from pig and cow erythrocyte glycoproteins and human milk fat globule membrane glycoproteins although the complete structure was not elucidated. In all native glycoprotein fractions, the unsubstituted disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosamine was found to be present to different extents. Haemagglutination inhibition tests against human anti-T serum, Arachis hypogoea and Vicia graminea by desialylated glycoproteins showed the presence of the T-antigen, confirming the chemical findings. Inhibition was found to be proportional to the chemically detected amounts of disaccharide in each fraction. Evidence for a second carbohydrate chain in horse, sheep and human erythrocyte glycoproteins with a sialic acid substituted N-acetylgalactosamine residue as the terminal sequence was obtained using the agglutinin from Helix pomatia.", "contents": "Alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes. Phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosaminital after treatment with alkaline borohydride. Cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. After gel filtration, a tetrasaccharide was isolated from horse and sheep glycoproteins containing the disaccharide plus two molecules of sialic acid. Periodate oxidation together with paper chromatography of alkaline degraded fragments showed these two molecules of sialic acid to be linked to positions C3 and C6 of the galactosyl and N-acetylgalactosamine residues respectively. Evidence was obtained for a similar structure from pig and cow erythrocyte glycoproteins and human milk fat globule membrane glycoproteins although the complete structure was not elucidated. In all native glycoprotein fractions, the unsubstituted disaccharide beta-D-galactosyl (1-3)-N-acetyl-D-galactosamine was found to be present to different extents. Haemagglutination inhibition tests against human anti-T serum, Arachis hypogoea and Vicia graminea by desialylated glycoproteins showed the presence of the T-antigen, confirming the chemical findings. Inhibition was found to be proportional to the chemically detected amounts of disaccharide in each fraction. Evidence for a second carbohydrate chain in horse, sheep and human erythrocyte glycoproteins with a sialic acid substituted N-acetylgalactosamine residue as the terminal sequence was obtained using the agglutinin from Helix pomatia.", "PMID": 963062}
{"id": "pubmed23n0032_5368", "title": "Preparation and characterization of calcium-binding and other hydrophobic proteins from synaptic membranes.", "content": "A number of hydrophobic proteins have been separated and purified to varying degrees from synaptic membranes derived from bovine brain. The proteins, which have been obtained using preparative acrylamide gel electrophoresis, have been analyzed for molecular weight, amino acid composition, peptide mapping, N-terminal amino acids, and for their ability to bind calcium and ATP. A number of the proteins bound calcium, the greatest binding being associated with a component having a molecular weight of 1.6 - 10(4), a binding capacity of 4 calcium/molecule, and a Km of 1.5 - 10(-5) M. An acidic tryptic peptide derived from this protein was evidently responsible for the calcium-binding. ATP binding appeared to be confined largely to the higher molecular weight proteins. From the peptide mapping there appears to be a similar acidic component in a number of the proteins exhibiting calcium-binding. ATP-binding was associated mainly with the high molecular weight proteins, particularly those which consisted of numerous basic tryptic peptides.", "contents": "Preparation and characterization of calcium-binding and other hydrophobic proteins from synaptic membranes. A number of hydrophobic proteins have been separated and purified to varying degrees from synaptic membranes derived from bovine brain. The proteins, which have been obtained using preparative acrylamide gel electrophoresis, have been analyzed for molecular weight, amino acid composition, peptide mapping, N-terminal amino acids, and for their ability to bind calcium and ATP. A number of the proteins bound calcium, the greatest binding being associated with a component having a molecular weight of 1.6 - 10(4), a binding capacity of 4 calcium/molecule, and a Km of 1.5 - 10(-5) M. An acidic tryptic peptide derived from this protein was evidently responsible for the calcium-binding. ATP binding appeared to be confined largely to the higher molecular weight proteins. From the peptide mapping there appears to be a similar acidic component in a number of the proteins exhibiting calcium-binding. ATP-binding was associated mainly with the high molecular weight proteins, particularly those which consisted of numerous basic tryptic peptides.", "PMID": 963063}
{"id": "pubmed23n0032_5369", "title": "The chromaffin granule surface. Localization of carbohydrate on the cytoplasmic surface of an intracellular organelle.", "content": "Intact chromaffin granules from bovine adrenal medulla are shown to have complex carbohydrates on their external (cytoplasmic) surface. This is demonstrated by the facts (1) that granules can be agglutinated by wheat germ agglutinin, and (2) that significant amounts of sialic acid can be removed from the granule surface with neuraminidase. Glycoproteins located in the granule membrane, and not glycolipids, are the molecules that mediate wheat germ agglutinin agglutination. The possible involvement of granule surface carbohydrate in the process of exocytosis is discussed.", "contents": "The chromaffin granule surface. Localization of carbohydrate on the cytoplasmic surface of an intracellular organelle. Intact chromaffin granules from bovine adrenal medulla are shown to have complex carbohydrates on their external (cytoplasmic) surface. This is demonstrated by the facts (1) that granules can be agglutinated by wheat germ agglutinin, and (2) that significant amounts of sialic acid can be removed from the granule surface with neuraminidase. Glycoproteins located in the granule membrane, and not glycolipids, are the molecules that mediate wheat germ agglutinin agglutination. The possible involvement of granule surface carbohydrate in the process of exocytosis is discussed.", "PMID": 963064}
{"id": "pubmed23n0032_5370", "title": "The affinity of rat liver ribosome subunits for degranulated rough microsomal membranes.", "content": "Potassium and magnesium ion concentrations affected the extent but not the specificity of binding in vitro of 60-S and 40-S ribosome subunits to degranulated rough microsomal membranes from rat liver. Scatchard plots revealed that under ionic conditions most likely to resemble those in vivo, the affinity constants for binding 60-S subunits were approximately four-times greater than those characterizing 40-S subunit binding. Further, the extent to which subunits bound at saturation was close to the level of ribosomes present in intact membranes.", "contents": "The affinity of rat liver ribosome subunits for degranulated rough microsomal membranes. Potassium and magnesium ion concentrations affected the extent but not the specificity of binding in vitro of 60-S and 40-S ribosome subunits to degranulated rough microsomal membranes from rat liver. Scatchard plots revealed that under ionic conditions most likely to resemble those in vivo, the affinity constants for binding 60-S subunits were approximately four-times greater than those characterizing 40-S subunit binding. Further, the extent to which subunits bound at saturation was close to the level of ribosomes present in intact membranes.", "PMID": 963065}
{"id": "pubmed23n0032_5371", "title": "Voltage dependent potassium fluxes and the significance of action potentials in Acetabularia.", "content": "Membrane potential, Vm, and K+ (86Rb+) fluxes have been measured simultaneously on individual cells of Acetabularia mediterranea. During resting state (resting potential approx. -170 mV) the K+ influx amounts to 0.24-0.6 pmol-cm-2-s-1 and the K+ efflux to 0.2-1.5 pmol-cm-2s-1. According to the K+ concentrations inside and outside the cell (40:1) the voltage dependent K+ flux (zero at Vm = EK = -90 mV) is stimulated approx. 40-fold for Vm more positive than EK. It is calculated that during one action potential (temporary depolarization to Vm more positive than EK) a cell looses the same amount of K+, which leaks in during 10-20 min in the resting state (Vm = -170 mV). Since action potentials occur spontaneously in Acetabularia, they are therefore suggested to have a significant function for the K+ balance of this alga.", "contents": "Voltage dependent potassium fluxes and the significance of action potentials in Acetabularia. Membrane potential, Vm, and K+ (86Rb+) fluxes have been measured simultaneously on individual cells of Acetabularia mediterranea. During resting state (resting potential approx. -170 mV) the K+ influx amounts to 0.24-0.6 pmol-cm-2-s-1 and the K+ efflux to 0.2-1.5 pmol-cm-2s-1. According to the K+ concentrations inside and outside the cell (40:1) the voltage dependent K+ flux (zero at Vm = EK = -90 mV) is stimulated approx. 40-fold for Vm more positive than EK. It is calculated that during one action potential (temporary depolarization to Vm more positive than EK) a cell looses the same amount of K+, which leaks in during 10-20 min in the resting state (Vm = -170 mV). Since action potentials occur spontaneously in Acetabularia, they are therefore suggested to have a significant function for the K+ balance of this alga.", "PMID": 963066}
{"id": "pubmed23n0032_5372", "title": "Photo-activated inhibition of sulfate equilibrium exchange in human erythrocyte ghosts by a 4-azido-2-nitrobenzoate derivative of phlorizin.", "content": "Like phlorizin, two glycosidic esters of phlorizin, the 4-azido-2-nitrobenzoate (ANB-phlorizin) and the 2-nitrobenzoate (NB-phlorizin) were found to be effective inhibitors of SO42- equilibrium exchange at the outer but not at the inner membrane surface of the human erythrocyte ghost. After photolysis of ghost suspensions in the presence of extracellular ANB-phlorizin an irreversible inhibition of SO42- exchange was observed, while photolysis of intracellular ANB-phlorizin was without effect. After photolysis in the presence of extracellular or intracellular tritiated ANB-phlorizin gel electrophoresis of the labelled membranes revealed similar locations of binding. These findings suggest that the sidedness of action of ANB-phlorizin could not be related to inaccessibility of the inner membrane surface for the agent but that inhibition occurs via binding to fixed sites at the outer membrane surface that are not associated with a mobile carrier which crosses the membrane.", "contents": "Photo-activated inhibition of sulfate equilibrium exchange in human erythrocyte ghosts by a 4-azido-2-nitrobenzoate derivative of phlorizin. Like phlorizin, two glycosidic esters of phlorizin, the 4-azido-2-nitrobenzoate (ANB-phlorizin) and the 2-nitrobenzoate (NB-phlorizin) were found to be effective inhibitors of SO42- equilibrium exchange at the outer but not at the inner membrane surface of the human erythrocyte ghost. After photolysis of ghost suspensions in the presence of extracellular ANB-phlorizin an irreversible inhibition of SO42- exchange was observed, while photolysis of intracellular ANB-phlorizin was without effect. After photolysis in the presence of extracellular or intracellular tritiated ANB-phlorizin gel electrophoresis of the labelled membranes revealed similar locations of binding. These findings suggest that the sidedness of action of ANB-phlorizin could not be related to inaccessibility of the inner membrane surface for the agent but that inhibition occurs via binding to fixed sites at the outer membrane surface that are not associated with a mobile carrier which crosses the membrane.", "PMID": 963067}
{"id": "pubmed23n0032_5373", "title": "Effects of electrical gradients on volume flows across gall bladder epithelium.", "content": "A volumetric method has been developed which permits continuous registration of volume flows across epithelial tissues. The method was applied to volume flow measurements across rabbit gall bladder epithelium. The rate of fluid reabsorption measured in this way was twice as high as previously observed in sac preparations of the gall bladder. This is probably due to better aeration and stirring of the mucosal solution. It was demonstrated that electrical gradients across the gall bladder induced volume flows towards the negative electrode. In non-transporting bladders volume flows were linearly related with current between 300 and 900 muA in both directions. However, volume flow rates were three times higher from mucosa to serosa than in the opposite direction. From the magnitude of polarization potentials, observed after switching off the current, the conclusion was reached that all of the current-induced volume flow is an osmotic flow due to salt polarization in the unstirred layers of the tissue. By implication, so-called streaming potentials observed during osmotic flows reflect solely polarization effects. In actively transporting gall bladders a 200 muA current increased or decreased the flow rate twice as much as expected from polarization effects alone. Therefore passage of current interfered directly with the active transport mechanism of gall bladder epithelium.", "contents": "Effects of electrical gradients on volume flows across gall bladder epithelium. A volumetric method has been developed which permits continuous registration of volume flows across epithelial tissues. The method was applied to volume flow measurements across rabbit gall bladder epithelium. The rate of fluid reabsorption measured in this way was twice as high as previously observed in sac preparations of the gall bladder. This is probably due to better aeration and stirring of the mucosal solution. It was demonstrated that electrical gradients across the gall bladder induced volume flows towards the negative electrode. In non-transporting bladders volume flows were linearly related with current between 300 and 900 muA in both directions. However, volume flow rates were three times higher from mucosa to serosa than in the opposite direction. From the magnitude of polarization potentials, observed after switching off the current, the conclusion was reached that all of the current-induced volume flow is an osmotic flow due to salt polarization in the unstirred layers of the tissue. By implication, so-called streaming potentials observed during osmotic flows reflect solely polarization effects. In actively transporting gall bladders a 200 muA current increased or decreased the flow rate twice as much as expected from polarization effects alone. Therefore passage of current interfered directly with the active transport mechanism of gall bladder epithelium.", "PMID": 963068}
{"id": "pubmed23n0032_5374", "title": "Oxidative damage of retinal rod outer segment membranes and the role of vitamin E.", "content": "Highly purified bovine rod outer segment membranes show loss of structural integrity under an air atmosphere. Obvious ultrastructural changes are preceded by increases in absorbance below 400 nm. These changes are inhibited by Ar or N2 atmospheres and appear to be due primarily to oxidative damage to the polyunsaturated fatty acids of the membrane lipids. Loss of polyunsaturated fatty acids, formation of malonaldehyde and fluorescent products characteristic of lipid oxidation accompany the spectral alterations. The elevated ultraviolet absorbance can largely be removed from the membranes by gentle extraction of the lipids using phospholipase C and hexane without changing the visible absorbance of rhodopsin. We have found a large seasonal variation in the endogenous level of alpha-tocopherol (vitamin E) in the bovine rod outer segment preparations. For much of the year we find that the rod outer segment membranes contain higher levels of alpha-tocopherol than have been previously reported in biological membranes. Rod outer segments which are low in endogenous tocopherol can be protected from oxygen damage by adding exogenous tocopherol. The rod outer segments are extremely susceptible to oxygen damage due to the unusually high content of polyunsaturated fatty acids in the membrane lipids. The presence of tocopherol inhibits oxygen damage but does not eliminate it. The tocopherol in the rod outer segments is consumed in air, thus complete protection from peroxidation in vitro requires an inert atmosphere as well as high levels of tocopherol. This work suggests that extensive precautions against oxidative degradation should also be employed in studies of other membrane systems where important deleterious effects of oxygen may be less obvious.", "contents": "Oxidative damage of retinal rod outer segment membranes and the role of vitamin E. Highly purified bovine rod outer segment membranes show loss of structural integrity under an air atmosphere. Obvious ultrastructural changes are preceded by increases in absorbance below 400 nm. These changes are inhibited by Ar or N2 atmospheres and appear to be due primarily to oxidative damage to the polyunsaturated fatty acids of the membrane lipids. Loss of polyunsaturated fatty acids, formation of malonaldehyde and fluorescent products characteristic of lipid oxidation accompany the spectral alterations. The elevated ultraviolet absorbance can largely be removed from the membranes by gentle extraction of the lipids using phospholipase C and hexane without changing the visible absorbance of rhodopsin. We have found a large seasonal variation in the endogenous level of alpha-tocopherol (vitamin E) in the bovine rod outer segment preparations. For much of the year we find that the rod outer segment membranes contain higher levels of alpha-tocopherol than have been previously reported in biological membranes. Rod outer segments which are low in endogenous tocopherol can be protected from oxygen damage by adding exogenous tocopherol. The rod outer segments are extremely susceptible to oxygen damage due to the unusually high content of polyunsaturated fatty acids in the membrane lipids. The presence of tocopherol inhibits oxygen damage but does not eliminate it. The tocopherol in the rod outer segments is consumed in air, thus complete protection from peroxidation in vitro requires an inert atmosphere as well as high levels of tocopherol. This work suggests that extensive precautions against oxidative degradation should also be employed in studies of other membrane systems where important deleterious effects of oxygen may be less obvious.", "PMID": 963069}
{"id": "pubmed23n0032_5375", "title": "Interaction of concanavalin A with rabbit thymocyte plasma membranes. Distinction between low affinity assoication and positively cooperative binding mediated by a specific glycoprotein.", "content": "1. We have analyzed the interaction of the mitogenic lectin, concanavalin A, with purified plasma membranes isolated from rabbit thymocytes. 2. Scatchard analyses show that in native membranes binding is positively cooperative at low concanavalin A concentrations and non-interacting at high lectin levels. 3. In contrast, membranes treated with 0.0064 M glutaraldehyde exhibit diphasic Scatchard plots, indicating the presence of high- and low-affinity binding sites. The high-affinity zone corresponds to the region of positive cooperativity in native membranes. 4. The number of high-affinity binding sites per cell-equivalent corresponds approximately to the number of glycoprotein (mol. wt. 55000) molecules (1-10(6)/cell), but account for less than 25% of the total lectin binding. 5. Treatment of membranes with 0.0064 M glutaraldehyde selectively crosslinks the glycoprotein (mol. wt. 55000) and its multimers, correlating directly with the modifications of concanavalin A-binding. 6. We conclude that high-affinity binding of concanavalin A to thymocyte membranes is a cooperative process mediated by the glycoprotein (mol. wt. 55000). We further conclude that the bulk of concanavalin A binding is through low-affinity associations, not involving specific membrane macromolecules.", "contents": "Interaction of concanavalin A with rabbit thymocyte plasma membranes. Distinction between low affinity assoication and positively cooperative binding mediated by a specific glycoprotein. 1. We have analyzed the interaction of the mitogenic lectin, concanavalin A, with purified plasma membranes isolated from rabbit thymocytes. 2. Scatchard analyses show that in native membranes binding is positively cooperative at low concanavalin A concentrations and non-interacting at high lectin levels. 3. In contrast, membranes treated with 0.0064 M glutaraldehyde exhibit diphasic Scatchard plots, indicating the presence of high- and low-affinity binding sites. The high-affinity zone corresponds to the region of positive cooperativity in native membranes. 4. The number of high-affinity binding sites per cell-equivalent corresponds approximately to the number of glycoprotein (mol. wt. 55000) molecules (1-10(6)/cell), but account for less than 25% of the total lectin binding. 5. Treatment of membranes with 0.0064 M glutaraldehyde selectively crosslinks the glycoprotein (mol. wt. 55000) and its multimers, correlating directly with the modifications of concanavalin A-binding. 6. We conclude that high-affinity binding of concanavalin A to thymocyte membranes is a cooperative process mediated by the glycoprotein (mol. wt. 55000). We further conclude that the bulk of concanavalin A binding is through low-affinity associations, not involving specific membrane macromolecules.", "PMID": 963070}
{"id": "pubmed23n0032_5376", "title": "Deuterated fatty acids as Raman spectroscopic probes of membrane structure.", "content": "Raman spectra are reported for the C-D stretching region of stearic acid-d35 bound in egg lecithin multilayers. The temperature dependence of the spectra shows that the linewidth of the C-D stretching bands is a sensitive and non-perturbative probe of membrane hydrocarbon chain conformation. The utility of this approach for studying lipid conformation in membranes containing a significant fraction of non-lipid component is discussed.", "contents": "Deuterated fatty acids as Raman spectroscopic probes of membrane structure. Raman spectra are reported for the C-D stretching region of stearic acid-d35 bound in egg lecithin multilayers. The temperature dependence of the spectra shows that the linewidth of the C-D stretching bands is a sensitive and non-perturbative probe of membrane hydrocarbon chain conformation. The utility of this approach for studying lipid conformation in membranes containing a significant fraction of non-lipid component is discussed.", "PMID": 963072}
{"id": "pubmed23n0032_5377", "title": "A measurement of the proton pump current generated by bacteriorhodopsin in black lipid membranes.", "content": "The light-induced electrical current generated by black lipid membranes containing bacteriorhodopsin from Halobacterium halobium has been measured directly. It is shown that a measurement of membrane potential can also be used to obtain the proton pump current developed during illumination. Evidence is presented that the charge movement across the membrane is associated with the release of protons in the photoreaction cycle of bacteriorhodopsin. The time variation of the pump current when the light is turned on suggests the rapid depopulation of some initially occupied state.", "contents": "A measurement of the proton pump current generated by bacteriorhodopsin in black lipid membranes. The light-induced electrical current generated by black lipid membranes containing bacteriorhodopsin from Halobacterium halobium has been measured directly. It is shown that a measurement of membrane potential can also be used to obtain the proton pump current developed during illumination. Evidence is presented that the charge movement across the membrane is associated with the release of protons in the photoreaction cycle of bacteriorhodopsin. The time variation of the pump current when the light is turned on suggests the rapid depopulation of some initially occupied state.", "PMID": 963073}
{"id": "pubmed23n0032_5378", "title": "Synthesis and turnover of leghaemoglobin in lupin root nodules.", "content": "1. The problem of whether leghaemoglobin is synthesized on plant of bacterial ribosomes in root nodules of yellow lupin has been examined. 2. Leghaemoglobin, soluble plant protein and soluble bacteroid protein were labelled with 14C administered by uptake of 14CO2. 3. Exposure of roots to 1 mM D-threo-chloramphenicol resulted in inhibition of soluble bacteroid protein synthesis, but leghaemoglobin synthesis and soluble plant protein synthesis were unaffected. This result is consistent with leghaemoglobin being synthesized on plant ribosomes. 4. After nitrogen-fixing plants had been supplied with a pulse of 14CO2, the decay of specific radioactivity of nodule protein fractions was observed. Leghaemoglobin had an apparent half-life of 18 days and is a stable protein in nitrogen-fixing yellow lupin nodules.", "contents": "Synthesis and turnover of leghaemoglobin in lupin root nodules. 1. The problem of whether leghaemoglobin is synthesized on plant of bacterial ribosomes in root nodules of yellow lupin has been examined. 2. Leghaemoglobin, soluble plant protein and soluble bacteroid protein were labelled with 14C administered by uptake of 14CO2. 3. Exposure of roots to 1 mM D-threo-chloramphenicol resulted in inhibition of soluble bacteroid protein synthesis, but leghaemoglobin synthesis and soluble plant protein synthesis were unaffected. This result is consistent with leghaemoglobin being synthesized on plant ribosomes. 4. After nitrogen-fixing plants had been supplied with a pulse of 14CO2, the decay of specific radioactivity of nodule protein fractions was observed. Leghaemoglobin had an apparent half-life of 18 days and is a stable protein in nitrogen-fixing yellow lupin nodules.", "PMID": 963075}
{"id": "pubmed23n0032_5379", "title": "Molecular weight determinations on the alpha-globin and beta-globin mRNAs.", "content": "The globin mRNAs containing between 30 and 40 polyadenylate residues can be separated from thos mRNAs containing longer poly(A) regions by Millipore filter binding. The molecular weights of the alpha-and beta-globin mRNAs containing this size class of poly(A) have beed determined by lectrophoresis on 3.7% polyacrylamide gels in the presence of 99% formamide. Because the number of adenylic acid residues in these mRNAs is known, the number of non-poly(A) nucleotides can be accurately calculated. The molecular weight of the beta-globin mRNA is 235 000 +/- 28 000 (736 +/- 88 nucleotides) and that of the alpha-globin mRNA is 208 900 +/- 43 870 (653 +/- 78 nucleotides). By subtracting the number of nucleotides in the coding and poly(A) regions, the number of non-coding nucleotides in the beta-globin mRNA were calculated to be 261, 69 more than the 193 present in the alpha-globin mRNA. Comparison of size estimates of newly synthesized globin mRNAs containing longer average lengths of poly(A) shhowed that there is no comparable processin of the 5' termini of the alpha-and beta-globin mRNAs concomitant with the stepwise degradation of the poly(A)  regions which occur as the mRNAs mature.", "contents": "Molecular weight determinations on the alpha-globin and beta-globin mRNAs. The globin mRNAs containing between 30 and 40 polyadenylate residues can be separated from thos mRNAs containing longer poly(A) regions by Millipore filter binding. The molecular weights of the alpha-and beta-globin mRNAs containing this size class of poly(A) have beed determined by lectrophoresis on 3.7% polyacrylamide gels in the presence of 99% formamide. Because the number of adenylic acid residues in these mRNAs is known, the number of non-poly(A) nucleotides can be accurately calculated. The molecular weight of the beta-globin mRNA is 235 000 +/- 28 000 (736 +/- 88 nucleotides) and that of the alpha-globin mRNA is 208 900 +/- 43 870 (653 +/- 78 nucleotides). By subtracting the number of nucleotides in the coding and poly(A) regions, the number of non-coding nucleotides in the beta-globin mRNA were calculated to be 261, 69 more than the 193 present in the alpha-globin mRNA. Comparison of size estimates of newly synthesized globin mRNAs containing longer average lengths of poly(A) shhowed that there is no comparable processin of the 5' termini of the alpha-and beta-globin mRNAs concomitant with the stepwise degradation of the poly(A)  regions which occur as the mRNAs mature.", "PMID": 963076}
{"id": "pubmed23n0032_5380", "title": "Preferential regulation of protein synthesis initiation complex formation by purine nucleotides.", "content": "A comparison of the affinities of eukaryotic initiation factor 2 and eukaryotic elongation factor 1 for GTP and GDP, and of the responses of initiation and elongation complex formation to various GTP mol fractions indicated that the initiation reaction was more sensitive to changes in the GTP: GDP ratio. In vitro regulation of the GTP: GDP ratio by the adenylate energy charge, a sensitive control parameter, also demonstrated a preference for regulation of formation of initiation complexes when compared to elongation complexes. These studies suggest that, based on the availability of energy, initiation is the rate-limiting step in the overall protein synthetic process.", "contents": "Preferential regulation of protein synthesis initiation complex formation by purine nucleotides. A comparison of the affinities of eukaryotic initiation factor 2 and eukaryotic elongation factor 1 for GTP and GDP, and of the responses of initiation and elongation complex formation to various GTP mol fractions indicated that the initiation reaction was more sensitive to changes in the GTP: GDP ratio. In vitro regulation of the GTP: GDP ratio by the adenylate energy charge, a sensitive control parameter, also demonstrated a preference for regulation of formation of initiation complexes when compared to elongation complexes. These studies suggest that, based on the availability of energy, initiation is the rate-limiting step in the overall protein synthetic process.", "PMID": 963077}
{"id": "pubmed23n0032_5381", "title": "Protein synthesis in chloroplasts. VII. Initiation of protein synthesis in isolated intact pea chloroplasts.", "content": "Isolated intact pea chloroplasts use light energy to synthesise N-formayl [35S]-methionylpuromycin when incubated with L-[35S]methionine and puromycin. Control experiments establish that this synthesis occurs on chloroplast ribosomes, and not on contaminating mitochondrial or bacterial ribosomes. The amount of N-formylmethionylpuromycin formed suggests that each messenger RNA that is being translated in vitro undergoes initiation at least twice. We conclude that isolated, intact pea chloroplasts carry out the initiation of protein synthesis, as well as the elongation and termination of polypeptide chains.", "contents": "Protein synthesis in chloroplasts. VII. Initiation of protein synthesis in isolated intact pea chloroplasts. Isolated intact pea chloroplasts use light energy to synthesise N-formayl [35S]-methionylpuromycin when incubated with L-[35S]methionine and puromycin. Control experiments establish that this synthesis occurs on chloroplast ribosomes, and not on contaminating mitochondrial or bacterial ribosomes. The amount of N-formylmethionylpuromycin formed suggests that each messenger RNA that is being translated in vitro undergoes initiation at least twice. We conclude that isolated, intact pea chloroplasts carry out the initiation of protein synthesis, as well as the elongation and termination of polypeptide chains.", "PMID": 963079}
{"id": "pubmed23n0032_5382", "title": "Ribosomal RNA synthesis in the mycelium of Phycomyces blakesleeanus. High molecular weight RNA.", "content": "Total RNA was extracted from 24 h old mycelia of Phycomyces blakesleeanus which had been grown in liquid culture. Heavy molecular weight RNA was fractionated on 2.6% polyacrylamide gels. 1. The main cytoplasmic ribosomal RNA fractions had molecular weights of 1.34 and 0.72-16(6). 2. [3H]Uridine pulse labelling of cultures revealed an initial ribosomal RNA precursor of molecular weight 2.5-10(6). 3. An intermediate precursor for the 1.34 - 10(6) dalton peak was found, having a molecular weight of 1.6-10(6). 4. Pulse-chase experiments, using [3H]methionine, showed the flow of synthesis as being (see article). 5. Mature 0.72-10(6) dalton RNA appeared prior to the 1.34-10(6) dalton RNA but no immediate precursor for it was detected.", "contents": "Ribosomal RNA synthesis in the mycelium of Phycomyces blakesleeanus. High molecular weight RNA. Total RNA was extracted from 24 h old mycelia of Phycomyces blakesleeanus which had been grown in liquid culture. Heavy molecular weight RNA was fractionated on 2.6% polyacrylamide gels. 1. The main cytoplasmic ribosomal RNA fractions had molecular weights of 1.34 and 0.72-16(6). 2. [3H]Uridine pulse labelling of cultures revealed an initial ribosomal RNA precursor of molecular weight 2.5-10(6). 3. An intermediate precursor for the 1.34 - 10(6) dalton peak was found, having a molecular weight of 1.6-10(6). 4. Pulse-chase experiments, using [3H]methionine, showed the flow of synthesis as being (see article). 5. Mature 0.72-10(6) dalton RNA appeared prior to the 1.34-10(6) dalton RNA but no immediate precursor for it was detected.", "PMID": 963080}
{"id": "pubmed23n0032_5383", "title": "Chain elongation and joining of DNA synthesized during hydroxyurea treatment of Chinese hamster cells.", "content": "We have previously presented evidence that hydroxyurea treatment of synchronized G1 Chinese hamster cells did not prevent the entry of cells into the DNA synthetic period but that the DNA synthesized during this period (in which total DNA synthesis was severely depressed) was quite small (Walters, R.A., Tobey, R.A. and Hildebrand, C.E. (1976) Biochem. Biophys. Res. Com. 69, 212-217). In view of the reported effects of hydroxyurea on deoxyribonucleoside metabolism and possible relationship to control of DNA replication (Bjursell, G. and Reichard, P. (1973) J. Biol. Chem. 248,3904-3909 and Walters, R.A., Tobey, R.A. and Ratliff, R.L. (1973) Biochim. Biophys. Acta 319, 336-347), we examined the fate of DNA synthesized during and shortly after hydroxyurea treatment to determine if this DNA exhibited any kinetic behavior which might be an indicator of aberrant synthesis. We found that, upon hydroxyurea removal, DNA grew at a linear rate of 0.98 +/- 0.12 - 10(6) dalton/min (0.98 +/- 0.12 mum/min) for about 2.3h. Beginning at 2.3 h, DNA with a molecular weight approx. 1.4 - 10(8) was very rapidly integrated into bulk DNA of greater than or equal to 3.5 - 10(8) daltons. The apparent growth rate of the 1.4 - 10(8) dalton DNA was approx. 10.6 mum/min. The data suggest that, at least for this DNA, joining into bulk DNA required one-third to one-half of the S period to begin and once begun, occurred very rapidly. The possibility of inegration of replicon clusters is considered.", "contents": "Chain elongation and joining of DNA synthesized during hydroxyurea treatment of Chinese hamster cells. We have previously presented evidence that hydroxyurea treatment of synchronized G1 Chinese hamster cells did not prevent the entry of cells into the DNA synthetic period but that the DNA synthesized during this period (in which total DNA synthesis was severely depressed) was quite small (Walters, R.A., Tobey, R.A. and Hildebrand, C.E. (1976) Biochem. Biophys. Res. Com. 69, 212-217). In view of the reported effects of hydroxyurea on deoxyribonucleoside metabolism and possible relationship to control of DNA replication (Bjursell, G. and Reichard, P. (1973) J. Biol. Chem. 248,3904-3909 and Walters, R.A., Tobey, R.A. and Ratliff, R.L. (1973) Biochim. Biophys. Acta 319, 336-347), we examined the fate of DNA synthesized during and shortly after hydroxyurea treatment to determine if this DNA exhibited any kinetic behavior which might be an indicator of aberrant synthesis. We found that, upon hydroxyurea removal, DNA grew at a linear rate of 0.98 +/- 0.12 - 10(6) dalton/min (0.98 +/- 0.12 mum/min) for about 2.3h. Beginning at 2.3 h, DNA with a molecular weight approx. 1.4 - 10(8) was very rapidly integrated into bulk DNA of greater than or equal to 3.5 - 10(8) daltons. The apparent growth rate of the 1.4 - 10(8) dalton DNA was approx. 10.6 mum/min. The data suggest that, at least for this DNA, joining into bulk DNA required one-third to one-half of the S period to begin and once begun, occurred very rapidly. The possibility of inegration of replicon clusters is considered.", "PMID": 963081}
{"id": "pubmed23n0032_5384", "title": "Histone inhibition of DNA synthesis in eukaryotic cells permeable to macromolecules.", "content": "Mouse L Cells, grown in suspension culture can be rendered permeable to exogenous deoxynucleoside triphosphates by a cold shock in a near isotonic buffer system. These cells use the deoxynucleotides to synthesize DNA in a semiconservative fashion. The addition of 0.05% Triton X-100 to this system increases the permeability of the cells so that exogenously supplied macromolecules gain access to the DNA. When DNAase and phosphodiesterase are added to the detergent-permeabilized cells, the cell DNA is rapidly degraded, demonstrating that the enzymes reach the DNA within the first 2 min of the incubation period. Addition of whole calf thymus histone or histone fractions to the detergent-permeabilized cells inhibits DNA synthesis. The lysine-rich histone, F is a more effective inhibitor than the arginine-rich histone, F3. The other histone fractions including the slightly lysine-rich fractions, F2a and F2b, are intermediate between F1 and F3 as inhibitors of DNA- synthesis. Kinetic analysis demonstrates that the added histones increase apparent Km and reduce V of DNA synthesis in the permeabilized cells. These studies suggest the possibility that histones alter the association of the DNA replication complex and the DNA template in a manner that reduces the rate of DNA synthesis.", "contents": "Histone inhibition of DNA synthesis in eukaryotic cells permeable to macromolecules. Mouse L Cells, grown in suspension culture can be rendered permeable to exogenous deoxynucleoside triphosphates by a cold shock in a near isotonic buffer system. These cells use the deoxynucleotides to synthesize DNA in a semiconservative fashion. The addition of 0.05% Triton X-100 to this system increases the permeability of the cells so that exogenously supplied macromolecules gain access to the DNA. When DNAase and phosphodiesterase are added to the detergent-permeabilized cells, the cell DNA is rapidly degraded, demonstrating that the enzymes reach the DNA within the first 2 min of the incubation period. Addition of whole calf thymus histone or histone fractions to the detergent-permeabilized cells inhibits DNA synthesis. The lysine-rich histone, F is a more effective inhibitor than the arginine-rich histone, F3. The other histone fractions including the slightly lysine-rich fractions, F2a and F2b, are intermediate between F1 and F3 as inhibitors of DNA- synthesis. Kinetic analysis demonstrates that the added histones increase apparent Km and reduce V of DNA synthesis in the permeabilized cells. These studies suggest the possibility that histones alter the association of the DNA replication complex and the DNA template in a manner that reduces the rate of DNA synthesis.", "PMID": 963082}
{"id": "pubmed23n0032_5385", "title": "Double strandedness of nascent DNA of a higher plant (Vicia faba).", "content": "After a pulse of 5-10 min with [3H]thymidine, labeled DNA extracted from embryonic axes of Vicia seeds sedimented as a rather homogeneous peak at approx. 10S in an alkaline sucrose density gradient, as described in our previous paper ((1975) Biochim. Biophys. Acta 395, 314-321). The sedimentation pattern of the same pulse-labeled DNA in a neutral gradient showed a wide range of sizes from approx. 14 S to more than 40 S. However, most of these labeled DNA components, including the 14-20 S shorter fragments, were shown to have a double-stranded structure by hydroxyapatite column chromatography. Further investigations on neutral sucrose gradients revealed a decreased occurrence after a chase, and final disappearance after a longer chase period of these shorter double-stranded fragments. A possible secondary structure of the newly synthesized DNA in a higher plant (Vicia faba) is discussed.", "contents": "Double strandedness of nascent DNA of a higher plant (Vicia faba). After a pulse of 5-10 min with [3H]thymidine, labeled DNA extracted from embryonic axes of Vicia seeds sedimented as a rather homogeneous peak at approx. 10S in an alkaline sucrose density gradient, as described in our previous paper ((1975) Biochim. Biophys. Acta 395, 314-321). The sedimentation pattern of the same pulse-labeled DNA in a neutral gradient showed a wide range of sizes from approx. 14 S to more than 40 S. However, most of these labeled DNA components, including the 14-20 S shorter fragments, were shown to have a double-stranded structure by hydroxyapatite column chromatography. Further investigations on neutral sucrose gradients revealed a decreased occurrence after a chase, and final disappearance after a longer chase period of these shorter double-stranded fragments. A possible secondary structure of the newly synthesized DNA in a higher plant (Vicia faba) is discussed.", "PMID": 963083}
{"id": "pubmed23n0032_5386", "title": "Free and membrane-bound ribosomes. VI. Distribution of free sulphydryl groups in rabbit reticulocyte ribosomes.", "content": "When free and membrane-bound ribosomes were titrated with N-ethyl[14C]-maleimide or 5,5'-dithio-bis(2-nitrobenzoic acid), 25 free SH groups were detected in  polysomes, 10 in the 60 S subunit and 14 in the 40 S subunits. When individual ribosomal proteins were analysed, one large subunit protein L14 was found to be strongly labelled compared to the other ribosomal proteins, in polysomes, monosomes and subunits. However, two additional protein spots from the small subunit were more radioactive in monosomes and subunits than inpolysomes. In all these studies, it was found that the distribution of free SH groups in free and membrane-bound ribosomes was identical except for the few proteins which were reported before to be specific for free or membrane-bound ribosomes (Fehlmann, ., Bellemare, G. and Godin, C. (1975) Biochim. Biophys. Acta 378, 119-124 and Fehlmann, M., Bellemare, G. and Godin, C. (1975) FEBS Lett. 59, 8-12).", "contents": "Free and membrane-bound ribosomes. VI. Distribution of free sulphydryl groups in rabbit reticulocyte ribosomes. When free and membrane-bound ribosomes were titrated with N-ethyl[14C]-maleimide or 5,5'-dithio-bis(2-nitrobenzoic acid), 25 free SH groups were detected in  polysomes, 10 in the 60 S subunit and 14 in the 40 S subunits. When individual ribosomal proteins were analysed, one large subunit protein L14 was found to be strongly labelled compared to the other ribosomal proteins, in polysomes, monosomes and subunits. However, two additional protein spots from the small subunit were more radioactive in monosomes and subunits than inpolysomes. In all these studies, it was found that the distribution of free SH groups in free and membrane-bound ribosomes was identical except for the few proteins which were reported before to be specific for free or membrane-bound ribosomes (Fehlmann, ., Bellemare, G. and Godin, C. (1975) Biochim. Biophys. Acta 378, 119-124 and Fehlmann, M., Bellemare, G. and Godin, C. (1975) FEBS Lett. 59, 8-12).", "PMID": 963084}
{"id": "pubmed23n0032_5387", "title": "[Distribution of identical amino acid residues in the primary structure of proteins].", "content": "It is shown that the distribution of identical amino acid residues in the primary structure of 83 non-homologous proteins containing about 14 000 amino acids is near to a casual one, i.e. it is determined by the amino acid composition. A characteristic feature of such distribution is an increased probability of a common grouping of amino acid residues.", "contents": "[Distribution of identical amino acid residues in the primary structure of proteins]. It is shown that the distribution of identical amino acid residues in the primary structure of 83 non-homologous proteins containing about 14 000 amino acids is near to a casual one, i.e. it is determined by the amino acid composition. A characteristic feature of such distribution is an increased probability of a common grouping of amino acid residues.", "PMID": 963085}
{"id": "pubmed23n0032_5388", "title": "[Selective broadening of NMR lines as a criterion in studying the interaction of Mn(II) with amino acids and peptides in D20].", "content": "A proton NMR study was carried out on complexes of Mn(II) with amino acids and peptides in aqueous solution. The theory, based on the Solomon-Bloembergen equations, was summarized and discussed. Different types of equilibria involving the anionic and zwittrionic forms of the amino acids were taken into account, together with the presence of outer- and inner-sphere species. The related correlation times were evaluated. Sarcosine, beta-alanine, threonine, tyrosine, valine, beta-alanine, proline, histidine and cystein complexes with Mn(II) were investigated on the basis of the NMR spectra. Moreover, diglycine and triglycine were studied in the presence of manganous ion at different pH value. The findings were discussed in terms of selective broadening of the NMR peaks and compared with data in the literature.", "contents": "[Selective broadening of NMR lines as a criterion in studying the interaction of Mn(II) with amino acids and peptides in D20]. A proton NMR study was carried out on complexes of Mn(II) with amino acids and peptides in aqueous solution. The theory, based on the Solomon-Bloembergen equations, was summarized and discussed. Different types of equilibria involving the anionic and zwittrionic forms of the amino acids were taken into account, together with the presence of outer- and inner-sphere species. The related correlation times were evaluated. Sarcosine, beta-alanine, threonine, tyrosine, valine, beta-alanine, proline, histidine and cystein complexes with Mn(II) were investigated on the basis of the NMR spectra. Moreover, diglycine and triglycine were studied in the presence of manganous ion at different pH value. The findings were discussed in terms of selective broadening of the NMR peaks and compared with data in the literature.", "PMID": 963086}
{"id": "pubmed23n0032_5389", "title": "[Optical measurements of nucleic acids in the presence of phenol and protein impurities].", "content": "Spectrophotometric method of determining the presence of phenol admixture in nucleic acid samples is presented. The method is based on determining the optical density ratio at 270 and 240 nm. This ratio is highly sensitive to the presence of phenol in solution. The method of determining nucleic acid concentration in the presence of phenol is also described. It is based on recording circular dichroism values at 265 nm for RNA and 275 nm for DNA. It is shown that the ratio of optical densities of nucleic acid samples at 260 and 280 nm is not a very sensitive criterion of the presence of proteins in solution. A much more sensitive characteristics is the ratio of circular dichroism values at 222 and 265 nm.", "contents": "[Optical measurements of nucleic acids in the presence of phenol and protein impurities]. Spectrophotometric method of determining the presence of phenol admixture in nucleic acid samples is presented. The method is based on determining the optical density ratio at 270 and 240 nm. This ratio is highly sensitive to the presence of phenol in solution. The method of determining nucleic acid concentration in the presence of phenol is also described. It is based on recording circular dichroism values at 265 nm for RNA and 275 nm for DNA. It is shown that the ratio of optical densities of nucleic acid samples at 260 and 280 nm is not a very sensitive criterion of the presence of proteins in solution. A much more sensitive characteristics is the ratio of circular dichroism values at 222 and 265 nm.", "PMID": 963087}
{"id": "pubmed23n0032_5390", "title": "[Formalin-induced chemiluminescence in model protein-lipid systems].", "content": "Mechanisms of formalin--induced luminescence of protein-lipid complexes have been studied. Free radical reactions of lipid oxidation were shown to be necessary for generating luminescence quanta. The luminescence intensity was mostly promoted by fluorescing products formed in the reoxidized system protein-lipid. Formalin-induced flash of luminescence in these systems may evidently serve as an express-method for analysing protein sample contamination with unsaturated lipids.", "contents": "[Formalin-induced chemiluminescence in model protein-lipid systems]. Mechanisms of formalin--induced luminescence of protein-lipid complexes have been studied. Free radical reactions of lipid oxidation were shown to be necessary for generating luminescence quanta. The luminescence intensity was mostly promoted by fluorescing products formed in the reoxidized system protein-lipid. Formalin-induced flash of luminescence in these systems may evidently serve as an express-method for analysing protein sample contamination with unsaturated lipids.", "PMID": 963088}
{"id": "pubmed23n0032_5391", "title": "[Determination of the mechanism of action of antioxidants in lipid systems from chemiluminescence in the presence of ferrous oxide].", "content": "Different action mechanisms of antioxidants affecting various stages of chemoluminescence developing in the lipid systems with ferrous ions are considered theoretically. Experimental studies are carried out on beta-naphtol and 5-oxitriptamine--antioxidants whose action mechanism in other systems has been investigated. It is concluded while comparing the experimental data with theoretical concepts that beta-naphtol in mitochondria and liposome suspensions binds the radicals according to the \"sticking\" mechanism, while 5-oxitriptamine reduces hydroperoxides.", "contents": "[Determination of the mechanism of action of antioxidants in lipid systems from chemiluminescence in the presence of ferrous oxide]. Different action mechanisms of antioxidants affecting various stages of chemoluminescence developing in the lipid systems with ferrous ions are considered theoretically. Experimental studies are carried out on beta-naphtol and 5-oxitriptamine--antioxidants whose action mechanism in other systems has been investigated. It is concluded while comparing the experimental data with theoretical concepts that beta-naphtol in mitochondria and liposome suspensions binds the radicals according to the \"sticking\" mechanism, while 5-oxitriptamine reduces hydroperoxides.", "PMID": 963089}
{"id": "pubmed23n0032_5392", "title": "[The simplest biochemical autogenerator--open enzyme reaction, S in equilibrium with P, with substrate inhibition].", "content": "Considered is the mathematical model of an open reversible enzymic reaction S equilibrium P with enzyme E inhibited by excess substrate S. The quasi-stationary output characteristic of the reaction, v(p), is shown to be of hysteretic Z-shaped form. It gives the rate of the product formation, v, as a function of of p=P], provided d[S]dt=0. Owing to hysteresis of the characteristic the reaction can exhibit 1 to 3 stationary states of different stability. Under specified conditions there occur self-oscillations in the reaction. Multiple stationary states and self-oscillations take place only with positive v, i.e. when the net flux is from S to P. The reaction considered is the simplest of all biochemical oscillators studied thus far.", "contents": "[The simplest biochemical autogenerator--open enzyme reaction, S in equilibrium with P, with substrate inhibition]. Considered is the mathematical model of an open reversible enzymic reaction S equilibrium P with enzyme E inhibited by excess substrate S. The quasi-stationary output characteristic of the reaction, v(p), is shown to be of hysteretic Z-shaped form. It gives the rate of the product formation, v, as a function of of p=P], provided d[S]dt=0. Owing to hysteresis of the characteristic the reaction can exhibit 1 to 3 stationary states of different stability. Under specified conditions there occur self-oscillations in the reaction. Multiple stationary states and self-oscillations take place only with positive v, i.e. when the net flux is from S to P. The reaction considered is the simplest of all biochemical oscillators studied thus far.", "PMID": 963090}
{"id": "pubmed23n0032_5393", "title": "[Oxidation-reduction potentials of chlorophyll pigments in photosynthesizing organisms on different levels of evolutionary development].", "content": "According to polarographically measured EOred of bacteriochlorophyll \"a\", chlorobium chlorophyll \"660\", \"b\" and \"a\" chlorophylls (-0.67 V, -0.73 V, -0.79 V, -0.86 V-n.h.e.) and literature data about Eoox of these pigments (+/-0.62 V, + 0.62 V, + 0.78 V, +0.77 V correspondingly) it has been shown that chemical energy stored in the singular photochemical set is increased in the evolution of the photosynthetic apparatus. It has apparently resulted in the possibility of evolutionary approach to utilization of more inert and more accessible (water) electron donors and favoured further development of photosynthesizing organisms.", "contents": "[Oxidation-reduction potentials of chlorophyll pigments in photosynthesizing organisms on different levels of evolutionary development]. According to polarographically measured EOred of bacteriochlorophyll \"a\", chlorobium chlorophyll \"660\", \"b\" and \"a\" chlorophylls (-0.67 V, -0.73 V, -0.79 V, -0.86 V-n.h.e.) and literature data about Eoox of these pigments (+/-0.62 V, + 0.62 V, + 0.78 V, +0.77 V correspondingly) it has been shown that chemical energy stored in the singular photochemical set is increased in the evolution of the photosynthetic apparatus. It has apparently resulted in the possibility of evolutionary approach to utilization of more inert and more accessible (water) electron donors and favoured further development of photosynthesizing organisms.", "PMID": 963091}
{"id": "pubmed23n0032_5394", "title": "[Experimental verification of a mathematical model of plasma protein and erythrocyte regeneration after blood loss].", "content": "The regeneration process after 40% bleeding is mathematically regular. Albumin, hemoglobin and red cells regeneration is expressed by the fraction-linear function. This law is true for albumin resotration only immediately after blood loss. Red cells and hemoglobin recovery begins only 3 days after acute bleeding, when albumin concentration rises by 90% of the normal level. Mathematical regularity of albumin regeneration was destroyed after the liver denervation or spleenectomy.", "contents": "[Experimental verification of a mathematical model of plasma protein and erythrocyte regeneration after blood loss]. The regeneration process after 40% bleeding is mathematically regular. Albumin, hemoglobin and red cells regeneration is expressed by the fraction-linear function. This law is true for albumin resotration only immediately after blood loss. Red cells and hemoglobin recovery begins only 3 days after acute bleeding, when albumin concentration rises by 90% of the normal level. Mathematical regularity of albumin regeneration was destroyed after the liver denervation or spleenectomy.", "PMID": 963092}
{"id": "pubmed23n0032_5395", "title": "[Biopolymer crystallization in malignant tumors].", "content": "It is shown by statistical analysis of structural studies of different polymers and polypeptides compounds, qualitative considerations and experimental studies of ascite strain of Ehrlich tumour that the growth of the tumour is accompanied by a specific course of crystallization of biopolymers.", "contents": "[Biopolymer crystallization in malignant tumors]. It is shown by statistical analysis of structural studies of different polymers and polypeptides compounds, qualitative considerations and experimental studies of ascite strain of Ehrlich tumour that the growth of the tumour is accompanied by a specific course of crystallization of biopolymers.", "PMID": 963093}
{"id": "pubmed23n0032_5396", "title": "[Structural changes in ganglioside micelles during interaction with serotonin].", "content": "Structure changes of ganglioside micelles in the process of serotonin bonding are considered. It was shown by viscosimetric and differential refraction studies that stretching of elliptical ganglioside micelles in the serotonin bonding process took place. The scope of micelles form changes depends on the pH value. An increase of the micelles summary negative charge at pH 9 increased the number of bonding centres and simultaneously caused deeper deformation of micelles due to the repulsion of the identically charged groups.", "contents": "[Structural changes in ganglioside micelles during interaction with serotonin]. Structure changes of ganglioside micelles in the process of serotonin bonding are considered. It was shown by viscosimetric and differential refraction studies that stretching of elliptical ganglioside micelles in the serotonin bonding process took place. The scope of micelles form changes depends on the pH value. An increase of the micelles summary negative charge at pH 9 increased the number of bonding centres and simultaneously caused deeper deformation of micelles due to the repulsion of the identically charged groups.", "PMID": 963094}
{"id": "pubmed23n0032_5397", "title": "[Distribution of small hydrophobic molecules in membranes. II. Heterogeneity of binding centers on a phospholipid bilayer surface].", "content": "4-dimethylaminochalcone (DMC), a hydrophobic uncharged fluorescent probe, was bound to a phospholipid bilayer surface and was distributed in different binding sites. Sites whose polar groups of phospholipid and hydration shell molecules were the least mobile had the greatest affinity to DMC. The increase DMC/phospholipid ratio resulted in DMC molecules getting bound to sites of a lesser affinity whose polar groups were more mobile. Cholesterol presence caused DMC binding to the first type of sites only.", "contents": "[Distribution of small hydrophobic molecules in membranes. II. Heterogeneity of binding centers on a phospholipid bilayer surface]. 4-dimethylaminochalcone (DMC), a hydrophobic uncharged fluorescent probe, was bound to a phospholipid bilayer surface and was distributed in different binding sites. Sites whose polar groups of phospholipid and hydration shell molecules were the least mobile had the greatest affinity to DMC. The increase DMC/phospholipid ratio resulted in DMC molecules getting bound to sites of a lesser affinity whose polar groups were more mobile. Cholesterol presence caused DMC binding to the first type of sites only.", "PMID": 963095}
{"id": "pubmed23n0032_5398", "title": "[2 mechanisms of membrane electroconductivity with tetrachloro-2-trifluoromethylbenzimidazole (TTFB)].", "content": "It has been found during galvanostatic measurements under the conditions of diffusion overtension that charge transfer through the membrane is carried out by the transfer of a proton from a neutral form to an anion (acidic region), or by transfer of a negative charge--dimer due to the formation of a hydrogen bond between them on the membrane border (alkaline region).", "contents": "[2 mechanisms of membrane electroconductivity with tetrachloro-2-trifluoromethylbenzimidazole (TTFB)]. It has been found during galvanostatic measurements under the conditions of diffusion overtension that charge transfer through the membrane is carried out by the transfer of a proton from a neutral form to an anion (acidic region), or by transfer of a negative charge--dimer due to the formation of a hydrogen bond between them on the membrane border (alkaline region).", "PMID": 963096}
{"id": "pubmed23n0032_5399", "title": "[Potential difference across subcellular particle membranes. I. Chemiosmotic and chemielectric mechanism of generation].", "content": "Transformation of light energy, of substrate oxidation and ATP hydrolysis energy into electric form during both oxidative and photo-phosphorylation can be described not only by means of chemiosmotic but also by chemielectric hypothesis. The latter hypothesis supposes, that dehydrated protons are taken up by electrostatic forces into the inner part of the membrane. Protons move into the input channels following electrons driven by chemical forces. Inside the membrane H+-ions are released into the output channels when electrons are transferred to the next electron carriers. Output H+-channels eject protons towards the other side of the membrane along the gradient of rising polarization of the channels. The inner resistance of chemielectric potential generators is lower than that of chemiosmotic one. A model of chemielectric mechanism is proposed. According to this mechanism electrons driving protons move along nonheme iron proteins and H+-ions are released into the output channels from semiquinones and hydroquinones.", "contents": "[Potential difference across subcellular particle membranes. I. Chemiosmotic and chemielectric mechanism of generation]. Transformation of light energy, of substrate oxidation and ATP hydrolysis energy into electric form during both oxidative and photo-phosphorylation can be described not only by means of chemiosmotic but also by chemielectric hypothesis. The latter hypothesis supposes, that dehydrated protons are taken up by electrostatic forces into the inner part of the membrane. Protons move into the input channels following electrons driven by chemical forces. Inside the membrane H+-ions are released into the output channels when electrons are transferred to the next electron carriers. Output H+-channels eject protons towards the other side of the membrane along the gradient of rising polarization of the channels. The inner resistance of chemielectric potential generators is lower than that of chemiosmotic one. A model of chemielectric mechanism is proposed. According to this mechanism electrons driving protons move along nonheme iron proteins and H+-ions are released into the output channels from semiquinones and hydroquinones.", "PMID": 963097}
{"id": "pubmed23n0032_5400", "title": "[Potential difference across subcellular particle membranes. II. Compensation method of measurement].", "content": "\"Zero-loop\" of the molecular potential transformer of submitochondrial particles (SMP) is separated from the remaining electron transfer chain by rotenone, and its e.m.f. ET=0,003+RT/2F in [NADP X H] [NAD+]/[NADP+] [NAD X H] volts is used in the compensative method of measurement of the potential difference across the SMP membrane (delta USMP). The phospholipid membrane, measuring the concentration of the penetrating anions in the solution contained SMP, is used as \"zero-indicators\". This concentration drops monotonically with increase in delta USMP. Delta USMP is equal to ET when the addition of substrates of transhydrogenase reaction with definite ET does not change the potential across phospholipid membrane.", "contents": "[Potential difference across subcellular particle membranes. II. Compensation method of measurement]. \"Zero-loop\" of the molecular potential transformer of submitochondrial particles (SMP) is separated from the remaining electron transfer chain by rotenone, and its e.m.f. ET=0,003+RT/2F in [NADP X H] [NAD+]/[NADP+] [NAD X H] volts is used in the compensative method of measurement of the potential difference across the SMP membrane (delta USMP). The phospholipid membrane, measuring the concentration of the penetrating anions in the solution contained SMP, is used as \"zero-indicators\". This concentration drops monotonically with increase in delta USMP. Delta USMP is equal to ET when the addition of substrates of transhydrogenase reaction with definite ET does not change the potential across phospholipid membrane.", "PMID": 963098}
{"id": "pubmed23n0032_5401", "title": "[Spectroscopic study of the interaction of cationic dyes with mitochondria].", "content": "Data are presented on the changes of absorption spectra of two cation dyes: nils blue and acridine orange while binding them with intact mitochondria of the rat liver in various functional states. It is found that when the dyes are bound with mitochondria characteristic changes proceed in the absorption spectra of dyes. These changes show that the formation of the dye molecule dimers is more intensive on the membrane than in solution. Energisation of mitochondria leads to a more intensive formation of acridine orange dimers, but the formation of nils blue dimers becomes less intensive. A suggestion is made about different nature of the interaction between these dyes and active groups of the mitochondria membrane.", "contents": "[Spectroscopic study of the interaction of cationic dyes with mitochondria]. Data are presented on the changes of absorption spectra of two cation dyes: nils blue and acridine orange while binding them with intact mitochondria of the rat liver in various functional states. It is found that when the dyes are bound with mitochondria characteristic changes proceed in the absorption spectra of dyes. These changes show that the formation of the dye molecule dimers is more intensive on the membrane than in solution. Energisation of mitochondria leads to a more intensive formation of acridine orange dimers, but the formation of nils blue dimers becomes less intensive. A suggestion is made about different nature of the interaction between these dyes and active groups of the mitochondria membrane.", "PMID": 963100}
{"id": "pubmed23n0032_5402", "title": "[Eserine-induced change in membrane potential of frog muscle fibers].", "content": "The action of eserine of acetylcholinesterase (AchE) reversible inhibitor of frog muscle fibres membrane potential (MP) under various physico-chemical conditions in external solution was studied. The data obtained show that changes of external pH in any direction decrease the depolarisation of the membrane produced by eserine. The dose-effect curve is linear at pH 7, but it has saturation at pH 6 and pH 9. Dependence of the membrane depolarisation in the presence of eserine upon calcium ions concentration in external solution is S-shape. Protonophore 3C1CCP (carbonilcianamid-m-3C1-phenylhydrazon) depolarises the membrane further in the presence of eserine. Valinomycin under these conditions completely restores the MP. Evidence is obtained that eserine reduces potassium permeability of the muscle membrane. It is supposed that membrane-bound AchE is involved in the ionic permeability regulation of the muscle membrane at rest.", "contents": "[Eserine-induced change in membrane potential of frog muscle fibers]. The action of eserine of acetylcholinesterase (AchE) reversible inhibitor of frog muscle fibres membrane potential (MP) under various physico-chemical conditions in external solution was studied. The data obtained show that changes of external pH in any direction decrease the depolarisation of the membrane produced by eserine. The dose-effect curve is linear at pH 7, but it has saturation at pH 6 and pH 9. Dependence of the membrane depolarisation in the presence of eserine upon calcium ions concentration in external solution is S-shape. Protonophore 3C1CCP (carbonilcianamid-m-3C1-phenylhydrazon) depolarises the membrane further in the presence of eserine. Valinomycin under these conditions completely restores the MP. Evidence is obtained that eserine reduces potassium permeability of the muscle membrane. It is supposed that membrane-bound AchE is involved in the ionic permeability regulation of the muscle membrane at rest.", "PMID": 963099}
{"id": "pubmed23n0032_5403", "title": "[Are the reciprocal sliding of protofibrils and the shortening of thick filaments during muscle contraction based on a common molecular mechanism?].", "content": "It is assumed that the back stroke of myosin bridges in a contracting fibre is determined by nucleotide binding. Then an inhibition of actomyosin dissociation can lead to the shortening of the thick filaments. Possible existence of a protein control system, suppressing this dissociation is discussed.", "contents": "[Are the reciprocal sliding of protofibrils and the shortening of thick filaments during muscle contraction based on a common molecular mechanism?]. It is assumed that the back stroke of myosin bridges in a contracting fibre is determined by nucleotide binding. Then an inhibition of actomyosin dissociation can lead to the shortening of the thick filaments. Possible existence of a protein control system, suppressing this dissociation is discussed.", "PMID": 963101}
{"id": "pubmed23n0032_5404", "title": "[Inactivation of skeletal muscle actin from newborn and adult rabbits in different conditions of polymerization and sample storage].", "content": "Changes in polymerization degree of actin of newborn and adult rabbit muscles while storing solutions were studied. It is found that under different polymerization and storage conditions including the presence of ATP, Ca++ ions, 2-metacaptoethanole of ethanole which slow down the inactivation of actin the latter proceeds faster in newborn rabbits than in adult ones. It is suggested that with different inactivation rate of actin of newborn and adult rabbits are associated the differences in their polymerization degree found earlier.", "contents": "[Inactivation of skeletal muscle actin from newborn and adult rabbits in different conditions of polymerization and sample storage]. Changes in polymerization degree of actin of newborn and adult rabbit muscles while storing solutions were studied. It is found that under different polymerization and storage conditions including the presence of ATP, Ca++ ions, 2-metacaptoethanole of ethanole which slow down the inactivation of actin the latter proceeds faster in newborn rabbits than in adult ones. It is suggested that with different inactivation rate of actin of newborn and adult rabbits are associated the differences in their polymerization degree found earlier.", "PMID": 963102}
{"id": "pubmed23n0032_5405", "title": "[Deformation and morphogenesis of Acetabularia cell membrane. I. Relation of deformation to genetic and geometric factors].", "content": "Relationships between the changes of geometric shape, volume and deformations of the apical growth part of acetabularia cell are studied. Jung module E approximately 25 kg/cm2, viscosity coefficient of the apex cell theta 4 - 10(4) kg-sec/cm2 and intracellular pressure P approximately 1 kg/cm2 are measured. Cell shape formation according to Waddington is concerned with simple physical factors which are under the genetic control. It is shown that uneven deformations with the loss of shape stability bring about periodic cell complications.", "contents": "[Deformation and morphogenesis of Acetabularia cell membrane. I. Relation of deformation to genetic and geometric factors]. Relationships between the changes of geometric shape, volume and deformations of the apical growth part of acetabularia cell are studied. Jung module E approximately 25 kg/cm2, viscosity coefficient of the apex cell theta 4 - 10(4) kg-sec/cm2 and intracellular pressure P approximately 1 kg/cm2 are measured. Cell shape formation according to Waddington is concerned with simple physical factors which are under the genetic control. It is shown that uneven deformations with the loss of shape stability bring about periodic cell complications.", "PMID": 963104}
{"id": "pubmed23n0032_5406", "title": "[Relation of light-induced fluorescence quenching of Atabrine to photosynthetic electron transport in isolated pea chloroplasts].", "content": "Light-induced fluorescence quenching of atebrin in isolated pea chloroplasts was studied. The observed quenching effect was shown to be influenced by DCMU, ADP+P and cofactors of photosynthetic electron transport. In the presence of ADP+P the amplitude of the quenching in control plants was not changed, whereas in the pea mutant blocked at PS2 it was considerably decreased. However the dark decay rate of the quenching effect in both cases increased 2-3 folds.", "contents": "[Relation of light-induced fluorescence quenching of Atabrine to photosynthetic electron transport in isolated pea chloroplasts]. Light-induced fluorescence quenching of atebrin in isolated pea chloroplasts was studied. The observed quenching effect was shown to be influenced by DCMU, ADP+P and cofactors of photosynthetic electron transport. In the presence of ADP+P the amplitude of the quenching in control plants was not changed, whereas in the pea mutant blocked at PS2 it was considerably decreased. However the dark decay rate of the quenching effect in both cases increased 2-3 folds.", "PMID": 963108}
{"id": "pubmed23n0032_5407", "title": "[Deformation and morphogenesis of Acetabularia cell membrane. II. Local mechanisms of regulating viscoelastic deformations].", "content": "Relationships between growth velocities of some parts of Acetabularia cell, ratio between the thickness of the cell membrane and apex diameter (its constructive rigidity), intracellular pressure and viscose-elastic deformations are used for physical substantiation of Waddington's concepts concerned with the existence and important search of local morphogenetic mechanisms. Their realization in a strongly deformated regions of Acetabularia cell membrane is shown. The relationship between viscosity, synthesis of the material and dimensions of region and intracellular pressure is found.", "contents": "[Deformation and morphogenesis of Acetabularia cell membrane. II. Local mechanisms of regulating viscoelastic deformations]. Relationships between growth velocities of some parts of Acetabularia cell, ratio between the thickness of the cell membrane and apex diameter (its constructive rigidity), intracellular pressure and viscose-elastic deformations are used for physical substantiation of Waddington's concepts concerned with the existence and important search of local morphogenetic mechanisms. Their realization in a strongly deformated regions of Acetabularia cell membrane is shown. The relationship between viscosity, synthesis of the material and dimensions of region and intracellular pressure is found.", "PMID": 963105}
{"id": "pubmed23n0032_5408", "title": "[Asymmetrical behavior of small intestine epithelium under osmotic pressure].", "content": "The influence of wide interval of osmotic gradients on transepithelial potential differences through small intestine were investigated. It was found, that ion permeability begins to rise at osmotic pressures equal to 0.200-0.250 osmomol on serosal side and 0.350-0.400 osomomol on mucosal side. The variatin in behaviour of osmotic potential differences caused by flows directed from mucosal solution to serosal one and in the reverse direction at smaller osmotic pressure is shown. An assumption was made that observed features of behaviour of osmotic potential differences were due to structural reorganization in intracellular junction.", "contents": "[Asymmetrical behavior of small intestine epithelium under osmotic pressure]. The influence of wide interval of osmotic gradients on transepithelial potential differences through small intestine were investigated. It was found, that ion permeability begins to rise at osmotic pressures equal to 0.200-0.250 osmomol on serosal side and 0.350-0.400 osomomol on mucosal side. The variatin in behaviour of osmotic potential differences caused by flows directed from mucosal solution to serosal one and in the reverse direction at smaller osmotic pressure is shown. An assumption was made that observed features of behaviour of osmotic potential differences were due to structural reorganization in intracellular junction.", "PMID": 963103}
{"id": "pubmed23n0032_5409", "title": "[Electric parameters and equivalent schematics for Acetabularia cells].", "content": "Was measured values of electric resistance (R) and capacity (C) of A. mediterranea cell at different levels of the membrane potential (MP). At the level -170 mV, RRP=4,3 KOm-cm2, at the level -80 mV REP=1,4 KOm-cm2. At both levels of MP C congruent to 1,3-10(3) mkF/cm2. A variant of equivalent electric scheme of the cell is suggested. Values of the elements comprising the scheme are presented, which allow an approximate reproduction of the value of MP levels and of transient processes between them.", "contents": "[Electric parameters and equivalent schematics for Acetabularia cells]. Was measured values of electric resistance (R) and capacity (C) of A. mediterranea cell at different levels of the membrane potential (MP). At the level -170 mV, RRP=4,3 KOm-cm2, at the level -80 mV REP=1,4 KOm-cm2. At both levels of MP C congruent to 1,3-10(3) mkF/cm2. A variant of equivalent electric scheme of the cell is suggested. Values of the elements comprising the scheme are presented, which allow an approximate reproduction of the value of MP levels and of transient processes between them.", "PMID": 963106}
{"id": "pubmed23n0032_5410", "title": "[Relation between light-induced intracellular and extracellular bioelectric reactions of plants].", "content": "The parallel registration of light-induced changes of electrical potential of the water plant Elodea has been made with the help of intracellular-microelectrode and extracellular-contact methods. It has been shown that the curve of the light-induced bioelectrical reaction (LBR) under the extracellular registration was similar to the curve of the first differential of light-induced changes of intracellular electrical potential (LIP), but with a negative sign. Thus, the kind of changing of LBR, as the first differential of LIP, may give information about intracellular potential of ground plants, for which the microelectrode way of registration was impossible. The likeness between LBR of Elodea and the ground plants studied suggests the general regularities in the light-induced changes of electrical potentials of different plants.", "contents": "[Relation between light-induced intracellular and extracellular bioelectric reactions of plants]. The parallel registration of light-induced changes of electrical potential of the water plant Elodea has been made with the help of intracellular-microelectrode and extracellular-contact methods. It has been shown that the curve of the light-induced bioelectrical reaction (LBR) under the extracellular registration was similar to the curve of the first differential of light-induced changes of intracellular electrical potential (LIP), but with a negative sign. Thus, the kind of changing of LBR, as the first differential of LIP, may give information about intracellular potential of ground plants, for which the microelectrode way of registration was impossible. The likeness between LBR of Elodea and the ground plants studied suggests the general regularities in the light-induced changes of electrical potentials of different plants.", "PMID": 963107}
{"id": "pubmed23n0032_5411", "title": "[Work of large information systems].", "content": "The problem of application of informational ideas to the study of large informational systems, such as brain, is considered. Analysis follows some ideas of Winograd and Kowan's theory of reliable computations in the presence of noise. In the beginning of paper the \"material analogy\" for intuitive understanding of Shannon's results is proposed. Afterwards there is given the formulation of coding theorem for the case of \"informational collective\"--a large group of informational sources and receivers. In conclusion it is shown that such properties of neuronal structures as \"stochastic\" neural connections are reasonable from the point of information theory.", "contents": "[Work of large information systems]. The problem of application of informational ideas to the study of large informational systems, such as brain, is considered. Analysis follows some ideas of Winograd and Kowan's theory of reliable computations in the presence of noise. In the beginning of paper the \"material analogy\" for intuitive understanding of Shannon's results is proposed. Afterwards there is given the formulation of coding theorem for the case of \"informational collective\"--a large group of informational sources and receivers. In conclusion it is shown that such properties of neuronal structures as \"stochastic\" neural connections are reasonable from the point of information theory.", "PMID": 963109}
{"id": "pubmed23n0032_5412", "title": "[Boundary conditions for excitation propagation equations which do not distort the picture of impulse movement].", "content": "New boundary conditions for the equations of excitation propagation are suggested. These conditions prescribe in the point in the end of the fiber the action potential which would be developed there, if half-infinite fiber would proceed further. The conditions suggested 1) in a number of cases allow to obtain an undistorted picture of excitation propagation, e.g. the picture when impulses fall out when a chain of impulses moves along the fiber; 2) save computer time and memory. Boundary conditions for syncitium and flat medium are considered.", "contents": "[Boundary conditions for excitation propagation equations which do not distort the picture of impulse movement]. New boundary conditions for the equations of excitation propagation are suggested. These conditions prescribe in the point in the end of the fiber the action potential which would be developed there, if half-infinite fiber would proceed further. The conditions suggested 1) in a number of cases allow to obtain an undistorted picture of excitation propagation, e.g. the picture when impulses fall out when a chain of impulses moves along the fiber; 2) save computer time and memory. Boundary conditions for syncitium and flat medium are considered.", "PMID": 963110}
{"id": "pubmed23n0032_5413", "title": "[Modelling the reaction of the horizontal cell layer to non-uniform light].", "content": "A mathematical model of horizontal cells in fish retina is constructed. Some conclusions and hypotheses concerning potential distribution and synaptic current are discussed.", "contents": "[Modelling the reaction of the horizontal cell layer to non-uniform light]. A mathematical model of horizontal cells in fish retina is constructed. Some conclusions and hypotheses concerning potential distribution and synaptic current are discussed.", "PMID": 963114}
{"id": "pubmed23n0032_5414", "title": "[Calculation of wave velocity in an excitable medium].", "content": "Analytical descriptions for the form and velocity values of sharp fronts in the impulse propagating in excitable medium are derived. In initial equations the form of nonlinear function is prescribed by the polynom of the third of fifth power.", "contents": "[Calculation of wave velocity in an excitable medium]. Analytical descriptions for the form and velocity values of sharp fronts in the impulse propagating in excitable medium are derived. In initial equations the form of nonlinear function is prescribed by the polynom of the third of fifth power.", "PMID": 963111}
{"id": "pubmed23n0032_5415", "title": "[Torsional movements of the human eye. I. Torsional movements on inclining the head towards the shoulder].", "content": "The characteristics of the torsional eye movements during head tilting were investigated. Different conditions were used in the experiments. The subjects were in sitting and lying positions in the light and in the dark. A new method of recording with the suction device of Yarbus and the simple optical system allowed to have records without some redundant components of movement. This made record processing less complex and more accurate. The torsional drift in the slow phase of the eye movement was found present under any conditions. When being recorded simultaneously both eyes in man were in identical and synchronous movement.", "contents": "[Torsional movements of the human eye. I. Torsional movements on inclining the head towards the shoulder]. The characteristics of the torsional eye movements during head tilting were investigated. Different conditions were used in the experiments. The subjects were in sitting and lying positions in the light and in the dark. A new method of recording with the suction device of Yarbus and the simple optical system allowed to have records without some redundant components of movement. This made record processing less complex and more accurate. The torsional drift in the slow phase of the eye movement was found present under any conditions. When being recorded simultaneously both eyes in man were in identical and synchronous movement.", "PMID": 963115}
{"id": "pubmed23n0032_5416", "title": "[EEG potential theory in a model with thin brain integuments. III. Source--tangential double layer in the cortex].", "content": "Estimates of EEG-potentials obtained in the model of polylayer spherical and flat cables indicate that the geometry of the skull has only little influence. Potentials of EEG of the radial dipole and the tangential double layer are expressed by simple formules in these models. A theoretical explanation concerning great dispersion of ECoG to EEG ratio and identity of EEG-potentials, registered by two electrodes spaced within about 2 cm of one another, is presented.", "contents": "[EEG potential theory in a model with thin brain integuments. III. Source--tangential double layer in the cortex]. Estimates of EEG-potentials obtained in the model of polylayer spherical and flat cables indicate that the geometry of the skull has only little influence. Potentials of EEG of the radial dipole and the tangential double layer are expressed by simple formules in these models. A theoretical explanation concerning great dispersion of ECoG to EEG ratio and identity of EEG-potentials, registered by two electrodes spaced within about 2 cm of one another, is presented.", "PMID": 963112}
{"id": "pubmed23n0032_5417", "title": "[Moments in human leg joints during walking].", "content": "An automated method is developed to obtain the moments of internal forces in the leg joints during walking. A considerable variability of joints moments is observed, which sufficiently exceeds that of the kinematic parameters of walking. For normal walking three different types of joints moments can be distinguished. The formal foundation for the variability of the moments is given, and a hypothesis is proposed, according to which the variability is associated with the keeping of equilibrium.", "contents": "[Moments in human leg joints during walking]. An automated method is developed to obtain the moments of internal forces in the leg joints during walking. A considerable variability of joints moments is observed, which sufficiently exceeds that of the kinematic parameters of walking. For normal walking three different types of joints moments can be distinguished. The formal foundation for the variability of the moments is given, and a hypothesis is proposed, according to which the variability is associated with the keeping of equilibrium.", "PMID": 963113}
{"id": "pubmed23n0032_5418", "title": "CSF amine metabolites, clinical symptoms, and body movement in psychiatric patients.", "content": "The relationships between CSF monoamine metabolites (HVA and 5HIAA), nurses' ratings of clinical symptoms, and telemetered measures of motor movement of ten schizophrenic and ten depressed patients were investigated. There was a significant negative correlation between CSF 5HIAA and both agitation ratings and motor movement in the schizophrenics. CSF HVA correlated positively to anxiety and anger in the depressives. The schizophrenics had a significantly higher CSF HVA than the depressives which appeared unrelated to motor movement. The effects of serotonin turnover and arousal in schizophrenia and the association between CSF metabolite gradients, stress, motor movement, and biogenic amine levels in depression are discussed.", "contents": "CSF amine metabolites, clinical symptoms, and body movement in psychiatric patients. The relationships between CSF monoamine metabolites (HVA and 5HIAA), nurses' ratings of clinical symptoms, and telemetered measures of motor movement of ten schizophrenic and ten depressed patients were investigated. There was a significant negative correlation between CSF 5HIAA and both agitation ratings and motor movement in the schizophrenics. CSF HVA correlated positively to anxiety and anger in the depressives. The schizophrenics had a significantly higher CSF HVA than the depressives which appeared unrelated to motor movement. The effects of serotonin turnover and arousal in schizophrenia and the association between CSF metabolite gradients, stress, motor movement, and biogenic amine levels in depression are discussed.", "PMID": 963133}
{"id": "pubmed23n0032_5419", "title": "Alcoholism: averaged visual evoked response amplitude-intensity slope and symmetry in withdrawal.", "content": "Alcohol withdrawal has been characterized in terms of some EEG changes but seldom in terms of the averaged evoked response. Characteristics of the AER, such as amplitude, latency, and the least-squares slope of the AER amplitude as a function of stimulus intensity, have been found to change as a function of arousal and to separate some psychiatric groups. Seventeen alcoholics in withdrawal, 27 stabilized alcoholics, and 30 control volunteer subjects participated in an experiment to determine the changes in AER characteristics in withdrawal. Four stimulus intensities were presented to the subjects from a Grass PS 22 photostimulator with the flash sources 102 cm from the nasion. AER amplitude, the slope of the amplitude as a function of stimulus intensity, and bilateral symmetry scores were calculated for group comparison. Withdrawal and stabilized alcoholics were found to have significantly greater AER amplitudes at all intensity levels. Greater A-I slope from the right hemisphere and higher symmetry were found for withdrawal alcoholics than the other two groups.", "contents": "Alcoholism: averaged visual evoked response amplitude-intensity slope and symmetry in withdrawal. Alcohol withdrawal has been characterized in terms of some EEG changes but seldom in terms of the averaged evoked response. Characteristics of the AER, such as amplitude, latency, and the least-squares slope of the AER amplitude as a function of stimulus intensity, have been found to change as a function of arousal and to separate some psychiatric groups. Seventeen alcoholics in withdrawal, 27 stabilized alcoholics, and 30 control volunteer subjects participated in an experiment to determine the changes in AER characteristics in withdrawal. Four stimulus intensities were presented to the subjects from a Grass PS 22 photostimulator with the flash sources 102 cm from the nasion. AER amplitude, the slope of the amplitude as a function of stimulus intensity, and bilateral symmetry scores were calculated for group comparison. Withdrawal and stabilized alcoholics were found to have significantly greater AER amplitudes at all intensity levels. Greater A-I slope from the right hemisphere and higher symmetry were found for withdrawal alcoholics than the other two groups.", "PMID": 963134}
{"id": "pubmed23n0032_5420", "title": "Monoamine oxidase inhibitors: potential for drug abuse.", "content": "Both the amphetamines and MAO inhibitors share common clinical and pharmacological properties, namely, (i) to clinically induce euphoriant-stimulating type and psychotomimetic effects in certain individuals, and (ii) to increase, albeit by different mechanisms, the amount of functionally available neurotransmitter (catecholamines and indoleamines) at the receptor site. The present data now indicate that, like the amphetamines, the use of MAO inhibitors can be clinically associated with dependence-tolerance. Perhaps these clinical findings will converge with other clinical-biochemical data in helping to define the specific amine(s) responsible for not only the clinical effects of these drugs but also the etiopathogenesis of major psychiatric illnesses such as the affective disorders and schizophrenia.", "contents": "Monoamine oxidase inhibitors: potential for drug abuse. Both the amphetamines and MAO inhibitors share common clinical and pharmacological properties, namely, (i) to clinically induce euphoriant-stimulating type and psychotomimetic effects in certain individuals, and (ii) to increase, albeit by different mechanisms, the amount of functionally available neurotransmitter (catecholamines and indoleamines) at the receptor site. The present data now indicate that, like the amphetamines, the use of MAO inhibitors can be clinically associated with dependence-tolerance. Perhaps these clinical findings will converge with other clinical-biochemical data in helping to define the specific amine(s) responsible for not only the clinical effects of these drugs but also the etiopathogenesis of major psychiatric illnesses such as the affective disorders and schizophrenia.", "PMID": 963135}
{"id": "pubmed23n0032_5421", "title": "Subjective wellbeing and temporal patterns of sympathetic-adrenal medullary activity.", "content": "Sixteen male subjects performed an audiovisual-conflict during 1 h of an ordinary day of work. Adrenaline and noradrenaline excretion and subjective reactions were measured before, during, and after the test period as well as at corresponding points in time in a control day of ordinary work with interruption. This program was carried out on two occasions, which were expected to differ with respect to the subjects' general mental condition and wellbeing. There were large intraindividual as well as interindividual variations in the rate at which adrenaline output decreased after the stress period. The results support the hypothesis that rapid recovery to baseline levels is positively related to a relaxed, alert and subjectively pleasant state of mind.", "contents": "Subjective wellbeing and temporal patterns of sympathetic-adrenal medullary activity. Sixteen male subjects performed an audiovisual-conflict during 1 h of an ordinary day of work. Adrenaline and noradrenaline excretion and subjective reactions were measured before, during, and after the test period as well as at corresponding points in time in a control day of ordinary work with interruption. This program was carried out on two occasions, which were expected to differ with respect to the subjects' general mental condition and wellbeing. There were large intraindividual as well as interindividual variations in the rate at which adrenaline output decreased after the stress period. The results support the hypothesis that rapid recovery to baseline levels is positively related to a relaxed, alert and subjectively pleasant state of mind.", "PMID": 963139}
{"id": "pubmed23n0032_5422", "title": "Organismic, stimulus and task determinants of phasic and tonic heart rate and skin conductance changes.", "content": "The effects of three variables (perceptual style 'leveling--sharpening', stimulus intensity and task) on tonic and phasic heart rate and skin conductance responses were studied. 24 levelers and 24 sharpeners received a series of 25 auditory stimuli under two instructions (task): in one task subject had to listen carefully to possible small differences between tones (intake task) and in the other task the subject had to count back silently and could neglect the tones (rejection task). One third of each group received tones of 50 dB, one third 75 dB and one third 100 dB. Results show that task has a strong effect on tonic responses in the direction predicted from Lacey's intake--rejection hypothesis. There is hardly any effect of task on phasic responses: only at the first trial heart rate tends to decelerate more in the intake than in the rejection task. Stimulus intensity does not influence tonic responses but very strongly affects phasic responses: deceleration to 50 and 75 dB, acceleration to 100 dB. GSR amplitude increases monotonically as a function of stimulus intensity. There is no difference between levelers and sharpeners with regard to tonic responses. Concerning phasic heart rate, sharpeners decelerate more than levelers and levelers have greater GSRs than sharpeners. It is suggested that tonic changes are relatively more under cognitive control, whereas phasic changes are more of a non-cognitive reflex-like nature. A relationship between the distinction arousal-activation as proposed by Pribram and McGuinness (1975) and the results of this experiment was suggested.", "contents": "Organismic, stimulus and task determinants of phasic and tonic heart rate and skin conductance changes. The effects of three variables (perceptual style 'leveling--sharpening', stimulus intensity and task) on tonic and phasic heart rate and skin conductance responses were studied. 24 levelers and 24 sharpeners received a series of 25 auditory stimuli under two instructions (task): in one task subject had to listen carefully to possible small differences between tones (intake task) and in the other task the subject had to count back silently and could neglect the tones (rejection task). One third of each group received tones of 50 dB, one third 75 dB and one third 100 dB. Results show that task has a strong effect on tonic responses in the direction predicted from Lacey's intake--rejection hypothesis. There is hardly any effect of task on phasic responses: only at the first trial heart rate tends to decelerate more in the intake than in the rejection task. Stimulus intensity does not influence tonic responses but very strongly affects phasic responses: deceleration to 50 and 75 dB, acceleration to 100 dB. GSR amplitude increases monotonically as a function of stimulus intensity. There is no difference between levelers and sharpeners with regard to tonic responses. Concerning phasic heart rate, sharpeners decelerate more than levelers and levelers have greater GSRs than sharpeners. It is suggested that tonic changes are relatively more under cognitive control, whereas phasic changes are more of a non-cognitive reflex-like nature. A relationship between the distinction arousal-activation as proposed by Pribram and McGuinness (1975) and the results of this experiment was suggested.", "PMID": 963140}
{"id": "pubmed23n0032_5423", "title": "Bilateral EEG differentiation of stimuli.", "content": "The EEG of both right and left hemispheres was monitored while subjects were presented with words, music, arithmetical problems and abstract pictures, (15 trials of each treatment, 9 sec per trial). The left side of the brain was most activated during presentation of words and arithmetic, whilst the right side of the brain was most activated during presentation of music. In addition, the right side was more activated during exposure to arithmetic than to words. The pictures were not differentiated by either side of the brain and it is possible that they had the effect of deactivating or relaxing subjects, since they yielded high abundance values compared with other stimuli. This study confirms earlier findings showing significant between-hemisphere ratios for arithmetic and words. In addition, however, the within-hemisphere analyses show that music affects the right hemisphere (without inducing between-hemisphere changes) and that arithmetic also activates the right hemisphere if compared with the non-activating effects of words.", "contents": "Bilateral EEG differentiation of stimuli. The EEG of both right and left hemispheres was monitored while subjects were presented with words, music, arithmetical problems and abstract pictures, (15 trials of each treatment, 9 sec per trial). The left side of the brain was most activated during presentation of words and arithmetic, whilst the right side of the brain was most activated during presentation of music. In addition, the right side was more activated during exposure to arithmetic than to words. The pictures were not differentiated by either side of the brain and it is possible that they had the effect of deactivating or relaxing subjects, since they yielded high abundance values compared with other stimuli. This study confirms earlier findings showing significant between-hemisphere ratios for arithmetic and words. In addition, however, the within-hemisphere analyses show that music affects the right hemisphere (without inducing between-hemisphere changes) and that arithmetic also activates the right hemisphere if compared with the non-activating effects of words.", "PMID": 963141}
{"id": "pubmed23n0032_5424", "title": "Signal onset and task variables in auditory evoked potentials.", "content": "Two auditory evoked potential studies were run to determine the effects of stimulus rise times on amplitude of N1-P2 onset and offset potentials. Onset potentials were reduced with increased onset durations. No consistent relationship was found in N1-P2 offset potentials as a function of stimulus rise time. The effects of listening tasks and stimulus duration were also examined.", "contents": "Signal onset and task variables in auditory evoked potentials. Two auditory evoked potential studies were run to determine the effects of stimulus rise times on amplitude of N1-P2 onset and offset potentials. Onset potentials were reduced with increased onset durations. No consistent relationship was found in N1-P2 offset potentials as a function of stimulus rise time. The effects of listening tasks and stimulus duration were also examined.", "PMID": 963142}
{"id": "pubmed23n0032_5425", "title": "A modification of the Johnson-Neyman technique comparing two regressions, applied to treatment effects dependent on baseline levels.", "content": "Treatment effects can often depend on baseline (pre-treatment) variables. This is ignored in many studies, or else the relationship is removed statistically by analysis of covariance. The latter method, however, assumes that slopes are equal and also loses information on the base-line contribution. Instead, the Johnson-Neyman technique does not assume that slopes are equal and furthermore permits examination of the baseline contribution. The method was devised for education research and is here extended to biological studies. However, the method assumes that variances are equal for the regressions, which might not be always so. The mmodification proposed accommodates inequalities of variance, whether intrinsic to the regressions or resulting from differences in group size. The Johnson--Neyman technique is discussed in relation to alternative analyses and, in appropriate situations, is considered to yield more information. Furthermore, with the refinement described, it involves even fewer assumptions and becomes more powerful.", "contents": "A modification of the Johnson-Neyman technique comparing two regressions, applied to treatment effects dependent on baseline levels. Treatment effects can often depend on baseline (pre-treatment) variables. This is ignored in many studies, or else the relationship is removed statistically by analysis of covariance. The latter method, however, assumes that slopes are equal and also loses information on the base-line contribution. Instead, the Johnson-Neyman technique does not assume that slopes are equal and furthermore permits examination of the baseline contribution. The method was devised for education research and is here extended to biological studies. However, the method assumes that variances are equal for the regressions, which might not be always so. The mmodification proposed accommodates inequalities of variance, whether intrinsic to the regressions or resulting from differences in group size. The Johnson--Neyman technique is discussed in relation to alternative analyses and, in appropriate situations, is considered to yield more information. Furthermore, with the refinement described, it involves even fewer assumptions and becomes more powerful.", "PMID": 963144}
{"id": "pubmed23n0032_5426", "title": "Telemetry in health care.", "content": "This article reviews the use of telemetry in health care. Monitoring of biological signals of medical importance such as the EKG, EEG, and EOG are described. Employment of telemetry in critical medical and surgical environs such as the delivery and operating suites is covered. The enormous importance of telemetry compatible with public communication systems such as voice grade telephone lines for remote medical care is discussed. The article does not enter into strictly engineering systems aspects in any detail; this was not the intent of the author. Nor is there any pretence to a completeness obviously not possible within its confines. It does concentrate on radio (wireless) telemetry transmissions over relatively great distances with freedom of patient movement.", "contents": "Telemetry in health care. This article reviews the use of telemetry in health care. Monitoring of biological signals of medical importance such as the EKG, EEG, and EOG are described. Employment of telemetry in critical medical and surgical environs such as the delivery and operating suites is covered. The enormous importance of telemetry compatible with public communication systems such as voice grade telephone lines for remote medical care is discussed. The article does not enter into strictly engineering systems aspects in any detail; this was not the intent of the author. Nor is there any pretence to a completeness obviously not possible within its confines. It does concentrate on radio (wireless) telemetry transmissions over relatively great distances with freedom of patient movement.", "PMID": 963166}
{"id": "pubmed23n0032_5427", "title": "Coating evolution with an implantable biological battery.", "content": "A cathode of smooth platinum in a right endoatrial position and an anode of Domal magnesium were used to construct a hybrid bioelectric battery designed to power a pacemaker. In use the cathode is gradually covered by a coating consisting of a crystalline layer and a layer of connective tissue. This coating is responsible for the system change in the reduction process at the cathode level: the transfer from an oxygen to a hydrogen system. This system change causes a voltage drop in the bioelectric battery. This paper deals with oxygen diffusion across the tissue layer, as well as with various spectral and chemical analyses. A high level of calcium and phosphorus in the different analyses carried out seems to show that the presence of these elements is related to the coating of the cathode. The effect on the formation of the coating of the position of the cathode is also discussed.", "contents": "Coating evolution with an implantable biological battery. A cathode of smooth platinum in a right endoatrial position and an anode of Domal magnesium were used to construct a hybrid bioelectric battery designed to power a pacemaker. In use the cathode is gradually covered by a coating consisting of a crystalline layer and a layer of connective tissue. This coating is responsible for the system change in the reduction process at the cathode level: the transfer from an oxygen to a hydrogen system. This system change causes a voltage drop in the bioelectric battery. This paper deals with oxygen diffusion across the tissue layer, as well as with various spectral and chemical analyses. A high level of calcium and phosphorus in the different analyses carried out seems to show that the presence of these elements is related to the coating of the cathode. The effect on the formation of the coating of the position of the cathode is also discussed.", "PMID": 963167}
{"id": "pubmed23n0032_5428", "title": "Knee forces during the activity of getting out of a chair with and without the aid of arms.", "content": "This is a preliminary study of the knee forces during the activity of getting out of a chair with and without the aid of arms. The equipment limited the study to the phase of the activity where no contact occurred between the subject and the chair seat. The model of the knee considered was two-dimensional, and quasi-static. Analysis showed that the forces acting in both compartments of the knee were comparable with those occurring during other non-strenuous activities such as level walking. It was also shown that the use of arms to aid rising from a chair causes considerable reduction in both joint and muscle forces.", "contents": "Knee forces during the activity of getting out of a chair with and without the aid of arms. This is a preliminary study of the knee forces during the activity of getting out of a chair with and without the aid of arms. The equipment limited the study to the phase of the activity where no contact occurred between the subject and the chair seat. The model of the knee considered was two-dimensional, and quasi-static. Analysis showed that the forces acting in both compartments of the knee were comparable with those occurring during other non-strenuous activities such as level walking. It was also shown that the use of arms to aid rising from a chair causes considerable reduction in both joint and muscle forces.", "PMID": 963168}
{"id": "pubmed23n0032_5429", "title": "Stochastic model of metastases formation.", "content": "A mathematical model is developed to describe the dynamics of the hematogenous metastatic process to the lung from a solid tumor growing in a peripheral body site. The accumulation of tumor cell clumps of various sizes in the pulmonary circulation and the formation of metastatic foci are described by a non-homogeneous, two-dimensional Markov process. An analytical solution is found for the special case of metastases produced by the intravenous injection of tumor cell clumps. The system is decoupled experimentally to determine the time-varying entrance rate of tumor cell clumps into the circulation from a growing fibrosarcoma and the number of metastatic foci produced by the intravenous injection of tumor clumps. Model validation is based on comparisons of model simulations with data for the development of metastatic foci and the probability of cure following tumor excision. The model is used to simulate hypothetical therapy to prevent tumor metastases.", "contents": "Stochastic model of metastases formation. A mathematical model is developed to describe the dynamics of the hematogenous metastatic process to the lung from a solid tumor growing in a peripheral body site. The accumulation of tumor cell clumps of various sizes in the pulmonary circulation and the formation of metastatic foci are described by a non-homogeneous, two-dimensional Markov process. An analytical solution is found for the special case of metastases produced by the intravenous injection of tumor cell clumps. The system is decoupled experimentally to determine the time-varying entrance rate of tumor cell clumps into the circulation from a growing fibrosarcoma and the number of metastatic foci produced by the intravenous injection of tumor clumps. Model validation is based on comparisons of model simulations with data for the development of metastatic foci and the probability of cure following tumor excision. The model is used to simulate hypothetical therapy to prevent tumor metastases.", "PMID": 963169}
{"id": "pubmed23n0032_5430", "title": "A stochastic model for censored-survival data in the presence of an auxiliary variable.", "content": "In clinical trials and other investigations of survival time, information is often available on a time-dependent event other than survival. An example of such an auxiliary event in cancer studies is objective progression of disease. While some patients expire without experiencing objective disease progression, others die after progression is observed. This paper proposes a stochastic model which utilizes this type of information in the evaluation of survival time. Our intentions in presenting this model are to provide a means of relating survival and another time-dependent event to one another (each of which may be used in the evaluation of a patient's condition), and to obtain more precise estimates of survival time by exploiting its relationship with this other event. The intrinsic aspects of the model are related to the semi-Markov model proposed by Weiss and Zelen [1965]. An important difference is that the present model incorporates incomplete (censored) observations as well as covariante variables. Analysis of the model via the method of maximum likelihood and its testability are discussed. The methods are applied to the results of a recent lung cancer study.", "contents": "A stochastic model for censored-survival data in the presence of an auxiliary variable. In clinical trials and other investigations of survival time, information is often available on a time-dependent event other than survival. An example of such an auxiliary event in cancer studies is objective progression of disease. While some patients expire without experiencing objective disease progression, others die after progression is observed. This paper proposes a stochastic model which utilizes this type of information in the evaluation of survival time. Our intentions in presenting this model are to provide a means of relating survival and another time-dependent event to one another (each of which may be used in the evaluation of a patient's condition), and to obtain more precise estimates of survival time by exploiting its relationship with this other event. The intrinsic aspects of the model are related to the semi-Markov model proposed by Weiss and Zelen [1965]. An important difference is that the present model incorporates incomplete (censored) observations as well as covariante variables. Analysis of the model via the method of maximum likelihood and its testability are discussed. The methods are applied to the results of a recent lung cancer study.", "PMID": 963170}
{"id": "pubmed23n0032_5431", "title": "A generalization of the one-sided two-sample Kolmogorov-Smirnov statistic for evaluating diagnostic tests.", "content": "Suppose a continuous diagnostic measurement is used to classify patients, and suppose E1 false negative errors and E2 false positive errors result. The quantities E1 and E2, and the total number of misclassifications, L = E1 + E2, depend on the choice of cut-off value. We have determined the null distribution of min L, where minimization is over all possible cut-off values. The statistic, min L, can be used as a quick one-sided two-sample test, and min L is also useful for evaluating publications which present only a 2 X 2 table of false positives, false negatives, true positives and true negatives. In such cases, one can use min L to assess the usefulness of the diagnostic measurement, even if one suspects that the authors chose that particular cut-off value which minimized L after looking at the data. We extend these results to a more general weighted loss L = vE1 + MUE2 where v and mu are positive integers, and we show that min L is a generalization of the one-sided two-sample Kolmogorov-Smirnov statistic, and, indeed, exactly equivalent to that statistic for appropriate choices of v and mu.", "contents": "A generalization of the one-sided two-sample Kolmogorov-Smirnov statistic for evaluating diagnostic tests. Suppose a continuous diagnostic measurement is used to classify patients, and suppose E1 false negative errors and E2 false positive errors result. The quantities E1 and E2, and the total number of misclassifications, L = E1 + E2, depend on the choice of cut-off value. We have determined the null distribution of min L, where minimization is over all possible cut-off values. The statistic, min L, can be used as a quick one-sided two-sample test, and min L is also useful for evaluating publications which present only a 2 X 2 table of false positives, false negatives, true positives and true negatives. In such cases, one can use min L to assess the usefulness of the diagnostic measurement, even if one suspects that the authors chose that particular cut-off value which minimized L after looking at the data. We extend these results to a more general weighted loss L = vE1 + MUE2 where v and mu are positive integers, and we show that min L is a generalization of the one-sided two-sample Kolmogorov-Smirnov statistic, and, indeed, exactly equivalent to that statistic for appropriate choices of v and mu.", "PMID": 963171}
{"id": "pubmed23n0032_5432", "title": "Life tables with concomitant information.", "content": "A regression model for the analysis of survival data adjusting for concomitant information is developed. The model presented can lead to the log linear exponential model (Glasser [1967]) and the life table regression model of Cox [1972]. In addition, the model described can be used to analyze data from the commonly employed actuarial life table. A discussion of the special case where one is comparing two survival curves is presented. The methods developed are illustrated using data from a clinical trial investigating treatments for lung cancer.", "contents": "Life tables with concomitant information. A regression model for the analysis of survival data adjusting for concomitant information is developed. The model presented can lead to the log linear exponential model (Glasser [1967]) and the life table regression model of Cox [1972]. In addition, the model described can be used to analyze data from the commonly employed actuarial life table. A discussion of the special case where one is comparing two survival curves is presented. The methods developed are illustrated using data from a clinical trial investigating treatments for lung cancer.", "PMID": 963172}
{"id": "pubmed23n0032_5433", "title": "Use of the logistic model in retrospective studies.", "content": "A logistic regression model is used to study the association between a dichotomous exposure variable and a disease. The method takes into account factors that may confound the association and leads to a quantitative study of the influence of factors which are related to the strength of the association. Special results are given for matched pair data. A case-control study relating post-menopausal estrogen use and endometrial cancer provides illustration.", "contents": "Use of the logistic model in retrospective studies. A logistic regression model is used to study the association between a dichotomous exposure variable and a disease. The method takes into account factors that may confound the association and leads to a quantitative study of the influence of factors which are related to the strength of the association. Special results are given for matched pair data. A case-control study relating post-menopausal estrogen use and endometrial cancer provides illustration.", "PMID": 963173}
{"id": "pubmed23n0032_5434", "title": "The efficiency of the multiple trial randomized response technique.", "content": "It has been proven theoretically that repeated administration of two-related-question randomized response technique (RRT) substantially increases the efficiency by reducing the variance of the estimate. The realtive efficiency of RRT to direct question with various number of trials per respondent under various degrees of truthful response and various conditions has also been examined. However, if the respondent becomes more concerned about his anonymity, then the mean square error may be larger. A field study of limited scope conducted in Taichung, Taiwan indicated that this multiple trial RRT model is at least as feasible as, if not more feasible than, the conventional single trial RRT. There was no sign suggesting cooperation of respondents had deteriorated because each was asked to repeat the procedures three times. If the number of trials did not exceed three, we believe that the increase in the cost of survey and \"exhaustion\" of respondents from repeated administration of the same set of questions would be negligible. Hence, we believe that this multiple trial technique is a feasible and useful survey method for study of various contemporary social problems of sensitive nature. More field testing, particularly in different socio-cultural settings, is necessary however, before a general conclusion may be drawn.", "contents": "The efficiency of the multiple trial randomized response technique. It has been proven theoretically that repeated administration of two-related-question randomized response technique (RRT) substantially increases the efficiency by reducing the variance of the estimate. The realtive efficiency of RRT to direct question with various number of trials per respondent under various degrees of truthful response and various conditions has also been examined. However, if the respondent becomes more concerned about his anonymity, then the mean square error may be larger. A field study of limited scope conducted in Taichung, Taiwan indicated that this multiple trial RRT model is at least as feasible as, if not more feasible than, the conventional single trial RRT. There was no sign suggesting cooperation of respondents had deteriorated because each was asked to repeat the procedures three times. If the number of trials did not exceed three, we believe that the increase in the cost of survey and \"exhaustion\" of respondents from repeated administration of the same set of questions would be negligible. Hence, we believe that this multiple trial technique is a feasible and useful survey method for study of various contemporary social problems of sensitive nature. More field testing, particularly in different socio-cultural settings, is necessary however, before a general conclusion may be drawn.", "PMID": 963174}
{"id": "pubmed23n0032_5435", "title": "Comparing the joint agreement of several raters with another rater.", "content": "In laboratory proficiency surveys which use reference laboratories for the evaluation of participant laboratories, a measure of the agreement of the participant laboratory with the reference laboratories is needed which considers the extent of agreement (or disagreement) among the reference laboratories themselves. In, a measure of nominal scale agreement, is proposed. In is interpreted as follows: Let a specimen be selected at random and rated by a reference laboratory which itself has been randomly selected from the n reference laboratories. If the specimen was also rated by the participant laboratory, this second rating would agree with the first at a rate In of the rate that would be obtained by a second randomly selected reference laboratory. An approximate (large sample) confidence interval for the ratio In is developed. In order to account for the more general case of scaled agreement, a weighted index of agreement is also considered.", "contents": "Comparing the joint agreement of several raters with another rater. In laboratory proficiency surveys which use reference laboratories for the evaluation of participant laboratories, a measure of the agreement of the participant laboratory with the reference laboratories is needed which considers the extent of agreement (or disagreement) among the reference laboratories themselves. In, a measure of nominal scale agreement, is proposed. In is interpreted as follows: Let a specimen be selected at random and rated by a reference laboratory which itself has been randomly selected from the n reference laboratories. If the specimen was also rated by the participant laboratory, this second rating would agree with the first at a rate In of the rate that would be obtained by a second randomly selected reference laboratory. An approximate (large sample) confidence interval for the ratio In is developed. In order to account for the more general case of scaled agreement, a weighted index of agreement is also considered.", "PMID": 963175}
{"id": "pubmed23n0032_5436", "title": "Testing for a single outlier from a general linear regression.", "content": "Several authors have considered the problem of detection of outliers from the general linear model Y = Xbeta + mu. Ellenberg [1973] among others, has advocated use of a detection method which involves examination of the set of internally standardized least squares residuals. Mickey [1974] and Snedecor and Cochran [1968], apparently concerned about the usefulness of an outlier detection method which is based on residual estimates that themselves are biassed by the presence of the outlier, have proposed two other alternatives. It is shown that the three approaches are exactly equivalent. A detection procedure is described which uses as its test statistic the maximum of the internally standardized least squares residuals, and upper and lower bounds for the percentage points of the test statistic are given by Bonferroni inequalities. The computations required to obtain these approximate percentage points are illustrated in a numerical example. Finally, a brief simulation study of the performance of the procedure illustrates that the power of the test can be influenced by the position of the outlier vis-a-vis the structure of the design matrix X.", "contents": "Testing for a single outlier from a general linear regression. Several authors have considered the problem of detection of outliers from the general linear model Y = Xbeta + mu. Ellenberg [1973] among others, has advocated use of a detection method which involves examination of the set of internally standardized least squares residuals. Mickey [1974] and Snedecor and Cochran [1968], apparently concerned about the usefulness of an outlier detection method which is based on residual estimates that themselves are biassed by the presence of the outlier, have proposed two other alternatives. It is shown that the three approaches are exactly equivalent. A detection procedure is described which uses as its test statistic the maximum of the internally standardized least squares residuals, and upper and lower bounds for the percentage points of the test statistic are given by Bonferroni inequalities. The computations required to obtain these approximate percentage points are illustrated in a numerical example. Finally, a brief simulation study of the performance of the procedure illustrates that the power of the test can be influenced by the position of the outlier vis-a-vis the structure of the design matrix X.", "PMID": 963176}
{"id": "pubmed23n0032_5437", "title": "Properties of diagnostic data distributions.", "content": "In applications of statistical methods to medical diagnosis, information on patients' diseases and symptoms is collected and the resulting data-base is used to diagnose new patients. The data-structure is complicated by a number of factors, two of which are examined here: selection bias and unstable population. Under reasonable conditions, no correction for selection bias is required when assessing probabilities for diseases based on symptom information, and it is suggested that these \"diagnostic distributions\" should form the principal object of study. Transformation of these distributions under changing population structure is considered and shown to take on a simple form in many situations. It is argued that the prevailing paradigm of diagnostic statistics, which concentrates on incidence of symptoms for given disease, is largely inappropriate and should be replaced by an emphasis on diagnostic distributions. The generalized logistic model is seen to fit naturally into the new framework.", "contents": "Properties of diagnostic data distributions. In applications of statistical methods to medical diagnosis, information on patients' diseases and symptoms is collected and the resulting data-base is used to diagnose new patients. The data-structure is complicated by a number of factors, two of which are examined here: selection bias and unstable population. Under reasonable conditions, no correction for selection bias is required when assessing probabilities for diseases based on symptom information, and it is suggested that these \"diagnostic distributions\" should form the principal object of study. Transformation of these distributions under changing population structure is considered and shown to take on a simple form in many situations. It is argued that the prevailing paradigm of diagnostic statistics, which concentrates on incidence of symptoms for given disease, is largely inappropriate and should be replaced by an emphasis on diagnostic distributions. The generalized logistic model is seen to fit naturally into the new framework.", "PMID": 963177}
{"id": "pubmed23n0032_5438", "title": "Skewness in commingled distributions.", "content": "A likelihood ratio test is given for distinguishing skewness from commingled distributions, using a power transform to remove skewness appropriately for each of the alternatives tested. The alternative hypotheses postulate that the transformed data are from one normal or a mixture of two or three normal homoscedastic distributions. Since each mixture has unique asymmetry, skewness is estimated simultaneously with the means, proportions and variance of components. Commingling cannot be rigorously proven in this way, as some other transform may provide a better approximation to normality. However, the error of asserting admixture whenever there is skewness has been avoided, and estimates of admixture parameters provide a basis for more conclusive tests in relatives or other populations. Two examples are given, one in which adjustment for skeweness left evidence of commingling.", "contents": "Skewness in commingled distributions. A likelihood ratio test is given for distinguishing skewness from commingled distributions, using a power transform to remove skewness appropriately for each of the alternatives tested. The alternative hypotheses postulate that the transformed data are from one normal or a mixture of two or three normal homoscedastic distributions. Since each mixture has unique asymmetry, skewness is estimated simultaneously with the means, proportions and variance of components. Commingling cannot be rigorously proven in this way, as some other transform may provide a better approximation to normality. However, the error of asserting admixture whenever there is skewness has been avoided, and estimates of admixture parameters provide a basis for more conclusive tests in relatives or other populations. Two examples are given, one in which adjustment for skeweness left evidence of commingling.", "PMID": 963179}
{"id": "pubmed23n0032_5439", "title": "Active immunological enhancement of sarcoma 180 in splenectomized Swiss mice.", "content": "Maximal enhancement of tumor growth was obtained in Swiss mice, with or without spleen, inoculated with S180 within a subcutaneously implanted glass cylinder and pretreated with soluble tumor antigen.", "contents": "Active immunological enhancement of sarcoma 180 in splenectomized Swiss mice. Maximal enhancement of tumor growth was obtained in Swiss mice, with or without spleen, inoculated with S180 within a subcutaneously implanted glass cylinder and pretreated with soluble tumor antigen.", "PMID": 963183}
{"id": "pubmed23n0032_5440", "title": "Transient antibodies to DNA polymerase in acute hepatitis B and related diseases.", "content": "Sera from patients with acute hepatitis, cirrhosis or chronic hepatitis, as well as sera from healthy carriers and controls were examined for HBS antigen, DNA polymerase activity and for antibodies to HBS, HBC and DNA polymerase. The data presented suggest that in acute hepatitis the DNA polymerase test enabled us to diagnose at least 20% more cases of hepatitis B than with the RIA but that the DNA polymerase test is of little value for the screening of blood donors since all the healthy carriers gave negative results. As concerns the antibodies to DNA polymerase they appeared in at least 50% of the patients with acute hepatitis, they were transient and only detectable at the early beginning of the disease. These antibodies were also found to be different from the anti-HBS and anti-HBC antibodies.", "contents": "Transient antibodies to DNA polymerase in acute hepatitis B and related diseases. Sera from patients with acute hepatitis, cirrhosis or chronic hepatitis, as well as sera from healthy carriers and controls were examined for HBS antigen, DNA polymerase activity and for antibodies to HBS, HBC and DNA polymerase. The data presented suggest that in acute hepatitis the DNA polymerase test enabled us to diagnose at least 20% more cases of hepatitis B than with the RIA but that the DNA polymerase test is of little value for the screening of blood donors since all the healthy carriers gave negative results. As concerns the antibodies to DNA polymerase they appeared in at least 50% of the patients with acute hepatitis, they were transient and only detectable at the early beginning of the disease. These antibodies were also found to be different from the anti-HBS and anti-HBC antibodies.", "PMID": 963184}
{"id": "pubmed23n0032_5441", "title": "Interactions of short chain aliphatic hydrocarbons with human blood and haemoglobin A solutions.", "content": "The solubilities  of methane, propane, butane and propylene were measured in human whole blood, human plasma and defatted haemoglobin solutions at 37 degrees C. For these four hydrocarbons the solubility coefficient (alpha = ml of gas STPD dissolved in one mal of the solvent under a pressure of one atmosphere) is higher in plasma than in an isoosmolar aqueous solution and higher in whole blood than in plasma. This is due to the presence of proteins and particularly of haemoglobin as shown by the dependence of alpha upon the concentration of haemoglobin in the medium. O2 capacity of human blood is not altered in the presence of these hydrocarbons and its affinity for O2 is slightly decreased.", "contents": "Interactions of short chain aliphatic hydrocarbons with human blood and haemoglobin A solutions. The solubilities  of methane, propane, butane and propylene were measured in human whole blood, human plasma and defatted haemoglobin solutions at 37 degrees C. For these four hydrocarbons the solubility coefficient (alpha = ml of gas STPD dissolved in one mal of the solvent under a pressure of one atmosphere) is higher in plasma than in an isoosmolar aqueous solution and higher in whole blood than in plasma. This is due to the presence of proteins and particularly of haemoglobin as shown by the dependence of alpha upon the concentration of haemoglobin in the medium. O2 capacity of human blood is not altered in the presence of these hydrocarbons and its affinity for O2 is slightly decreased.", "PMID": 963185}
{"id": "pubmed23n0032_5442", "title": "Intestinal colonization with lactobacilli strains in neutropenic patients.", "content": "In order to protect granulopenic patients against infections by pathogens from their own digestive tract, large amounts of lactobacilli were given to 5 patients whose intestinal flora had been suppressed by oral antibiotics. In all these cases, lactobacilli failed to prevent spontaneous recolonization of the gastrointestinal tract by enteric bacteria. Six attempts of colonization were made; in spite of the importance of the inoculum administered orally, large numbers of lactobacilli were recovered from stools in only two cases.", "contents": "Intestinal colonization with lactobacilli strains in neutropenic patients. In order to protect granulopenic patients against infections by pathogens from their own digestive tract, large amounts of lactobacilli were given to 5 patients whose intestinal flora had been suppressed by oral antibiotics. In all these cases, lactobacilli failed to prevent spontaneous recolonization of the gastrointestinal tract by enteric bacteria. Six attempts of colonization were made; in spite of the importance of the inoculum administered orally, large numbers of lactobacilli were recovered from stools in only two cases.", "PMID": 963186}
{"id": "pubmed23n0032_5443", "title": "[Effect of cycloleucine on renal uptake of dibasic amino acids and cystine].", "content": "Cycloleucinead ministration (a synthetic amino acid) to rats produces a selective hyperaminoaciduria bearing on dibasic amino acids (lysine, arginine, ornithine) and cystine. Interference of cycloleucine with tubular reabsorption of these amino acids was studied \"in vitro\", using rat kidney cortex slices. When dibasic amino acids and cystine are at physiological concentrations, the data indicate that cycloleucine decreases their intracellular accumulation. However, effect of cycloleucine \"in vitro\" is not specific whatsoever its concentration; similar results are obtained with neutral amino acids such as alpha-alanine and valine. Inhibitory effects are not modified by the presence of several amino acids in the incubation medium. The results are interpreted as evidence for a dissociation between \"in vitro\" cellular accumu-lation and \"in vivo\" transepithelial transport.", "contents": "[Effect of cycloleucine on renal uptake of dibasic amino acids and cystine]. Cycloleucinead ministration (a synthetic amino acid) to rats produces a selective hyperaminoaciduria bearing on dibasic amino acids (lysine, arginine, ornithine) and cystine. Interference of cycloleucine with tubular reabsorption of these amino acids was studied \"in vitro\", using rat kidney cortex slices. When dibasic amino acids and cystine are at physiological concentrations, the data indicate that cycloleucine decreases their intracellular accumulation. However, effect of cycloleucine \"in vitro\" is not specific whatsoever its concentration; similar results are obtained with neutral amino acids such as alpha-alanine and valine. Inhibitory effects are not modified by the presence of several amino acids in the incubation medium. The results are interpreted as evidence for a dissociation between \"in vitro\" cellular accumu-lation and \"in vivo\" transepithelial transport.", "PMID": 963187}
{"id": "pubmed23n0032_5444", "title": "An analysis of the effect of random and ageing mechanisms on the survival of platelets.", "content": "Human platelets survival curves make it possible to estimate two factors which could be held responsible for the disappearance of cells: a) random destruction b) ageing of the platelets. This study was devoted to non-thrombocytopenic patients with chronic vascular disease. In 40% of the cases, the platelet destruction was found to be due to normal ageing of the cells combined with a random destruction process. The estimation of both of these parameters is of interest for the clinical survey of these patients. The statistical analysis of the remaining 60% of the cases presented here has demonstrated a pure linear disappearance of the labelled platelets. This obsveration favours ageing as the cause of the destruction of the platelets.", "contents": "An analysis of the effect of random and ageing mechanisms on the survival of platelets. Human platelets survival curves make it possible to estimate two factors which could be held responsible for the disappearance of cells: a) random destruction b) ageing of the platelets. This study was devoted to non-thrombocytopenic patients with chronic vascular disease. In 40% of the cases, the platelet destruction was found to be due to normal ageing of the cells combined with a random destruction process. The estimation of both of these parameters is of interest for the clinical survey of these patients. The statistical analysis of the remaining 60% of the cases presented here has demonstrated a pure linear disappearance of the labelled platelets. This obsveration favours ageing as the cause of the destruction of the platelets.", "PMID": 963188}
{"id": "pubmed23n0032_5445", "title": "A simple method for the measurement of labelled compound incorporation into cells in culture.", "content": "A simple method for the measurement of labelled compound incorporation into cells in layer culture was developed. Compared to other methods it proves to spare time and to be more sensitive owing to the fact that cells are not detached from the culture vials until the end of the manipulation as these are dissolved in the scintillation medium together with the cells just before counting.", "contents": "A simple method for the measurement of labelled compound incorporation into cells in culture. A simple method for the measurement of labelled compound incorporation into cells in layer culture was developed. Compared to other methods it proves to spare time and to be more sensitive owing to the fact that cells are not detached from the culture vials until the end of the manipulation as these are dissolved in the scintillation medium together with the cells just before counting.", "PMID": 963189}
{"id": "pubmed23n0032_5446", "title": "Salivary immunoglobulin determinations: their diagnostic value in Sj\u00f6gren's syndrome.", "content": "Salivary immunoglobulins (IgA, IgG, IgM) determinations are performed on 74 patients with and without Sj\u00f6gren's syndrome (SS). In normal subjects IgA is the only immunoglobulin detected in saliva by classical immunodiffusion methods. Correlations between the presence of IgG and/or IgM in saliva and other functional and objective clinical parameters and hsitological aspects of minor salivary glands are studied. In SS the prescence of IgG and/or IgM is in close relationship with \"sialography index\" which is the most reliable clinical investigation. These results point out that salivary immunoglobulins determinations constitute a simple and specific complementary test for SS diagnosis; they may allow the clinicians to observe the progress of the disease or to evaluate effectiveness of drugs.", "contents": "Salivary immunoglobulin determinations: their diagnostic value in Sj\u00f6gren's syndrome. Salivary immunoglobulins (IgA, IgG, IgM) determinations are performed on 74 patients with and without Sj\u00f6gren's syndrome (SS). In normal subjects IgA is the only immunoglobulin detected in saliva by classical immunodiffusion methods. Correlations between the presence of IgG and/or IgM in saliva and other functional and objective clinical parameters and hsitological aspects of minor salivary glands are studied. In SS the prescence of IgG and/or IgM is in close relationship with \"sialography index\" which is the most reliable clinical investigation. These results point out that salivary immunoglobulins determinations constitute a simple and specific complementary test for SS diagnosis; they may allow the clinicians to observe the progress of the disease or to evaluate effectiveness of drugs.", "PMID": 963190}
{"id": "pubmed23n0032_5447", "title": "An attempt of treatment of malignant melanoma with bovine pineal tissue.", "content": "Seven incurable patients with disseminated malignant melanoma were injected (subcutaneously, sometimes intravenously) with homogenate of bovine pineal tissue. The daily injections of large quantities of pineal tissue appeared to be harmless for patients. Many-fold injections (during 9.5 months) of large quantities of pineal tissue (up to 3950 g) in 1 of 7 cases produced certain effect on subcutaneous metastases of malignant melanoma, i.e. causing reduction and even disappearance.", "contents": "An attempt of treatment of malignant melanoma with bovine pineal tissue. Seven incurable patients with disseminated malignant melanoma were injected (subcutaneously, sometimes intravenously) with homogenate of bovine pineal tissue. The daily injections of large quantities of pineal tissue appeared to be harmless for patients. Many-fold injections (during 9.5 months) of large quantities of pineal tissue (up to 3950 g) in 1 of 7 cases produced certain effect on subcutaneous metastases of malignant melanoma, i.e. causing reduction and even disappearance.", "PMID": 963195}
{"id": "pubmed23n0032_5448", "title": "Significance of dimers to the size of newly synthesized DNA in UV-irradiated Chinese hamster ovary cells.", "content": "DNA synthesized after UV irradiation is smaller than that in unirradiated cells even when pulse-labeling times are increased to compensate for the overall reduction in the rate of DNA replication. By isolating newly replicated DNA, incubating it with dimer-specific endonuclease from Micrococcus luteus, and analyzing it on alkaline sucrose gradients, we have been able to demonstrate that this DNA is synthesized in segments corresponding in size to the interdimer distance on the parental strand. In addition, the same DNA analyzed on neutral gradients shows no reduction in molecular weight as a result of UV irradiation and/or endonuclease digestion. Our data are thus inconsistent with the presence of \"gaps\" in newly synthesized DNA opposite the dimers on the parental strand. We suggest that if such gaps are produced as a result of delayed synthesis around dimers, they are filled before the growing point reaches the next dimer.", "contents": "Significance of dimers to the size of newly synthesized DNA in UV-irradiated Chinese hamster ovary cells. DNA synthesized after UV irradiation is smaller than that in unirradiated cells even when pulse-labeling times are increased to compensate for the overall reduction in the rate of DNA replication. By isolating newly replicated DNA, incubating it with dimer-specific endonuclease from Micrococcus luteus, and analyzing it on alkaline sucrose gradients, we have been able to demonstrate that this DNA is synthesized in segments corresponding in size to the interdimer distance on the parental strand. In addition, the same DNA analyzed on neutral gradients shows no reduction in molecular weight as a result of UV irradiation and/or endonuclease digestion. Our data are thus inconsistent with the presence of \"gaps\" in newly synthesized DNA opposite the dimers on the parental strand. We suggest that if such gaps are produced as a result of delayed synthesis around dimers, they are filled before the growing point reaches the next dimer.", "PMID": 963196}
{"id": "pubmed23n0032_5449", "title": "Chemotropism indices for polymorphonuclear leukocytes.", "content": "Trajectories of polymorphonuclear leukocytes which are responding to a chemical gradient are analyzed in order to deduce probability distributions of the angles between successive path segments. The turn angle probability distributions thus obtained are seen to be strongly dependent on the direction of locomotion prior to a turn, in that cells usually turn to maintain alignment along an axis directed towards the chemoattractant source. A mathematical model based on these observations is developed in order to understand the relationship between net chemotactic response and parameters characterizing stochastic movements of individual cells. In particular, the manner in which the chemotropism index depends on details of the turn-angle distributions is examined. When bias in the direction of turn is induced by a chemotactic field, transition from random motion to directed response occurs most abruptly if the turn-angle distribution is narrow. \"Accommodation,\" viz., a dependence of the mean angle of turn upon prior orientation, is found to have relatively little effect on the magnitude of the response.", "contents": "Chemotropism indices for polymorphonuclear leukocytes. Trajectories of polymorphonuclear leukocytes which are responding to a chemical gradient are analyzed in order to deduce probability distributions of the angles between successive path segments. The turn angle probability distributions thus obtained are seen to be strongly dependent on the direction of locomotion prior to a turn, in that cells usually turn to maintain alignment along an axis directed towards the chemoattractant source. A mathematical model based on these observations is developed in order to understand the relationship between net chemotactic response and parameters characterizing stochastic movements of individual cells. In particular, the manner in which the chemotropism index depends on details of the turn-angle distributions is examined. When bias in the direction of turn is induced by a chemotactic field, transition from random motion to directed response occurs most abruptly if the turn-angle distribution is narrow. \"Accommodation,\" viz., a dependence of the mean angle of turn upon prior orientation, is found to have relatively little effect on the magnitude of the response.", "PMID": 963197}
{"id": "pubmed23n0032_5450", "title": "Effects of light adaptation on the purple membrane structure of Halobacterium halobium.", "content": "Absorption, circular dichroism and optical rotatory dispersion of the bacteriorhodopsin containing purple membrane form Halobacterium halobium were studied in regard to the structural stability of this membrane during the photoisomerization of the retinal of the bacteriorhodopsin from the 13-cis to the all-trans configuration. The following conclusions were reached: (a) the macromolecular structure (protein-protein interaction which may result in the possible exciton interaction of the retinal pi-pi* (NV1) transition moments and protein-lipid interaction) are not significantly altered, (b) possibilities of delocalized conformation changes of the apoprotein involving secondary and/or tertiary structure can be ruled out, (c) localized secondary structure conformation changes of the apoprotein must be limited to the involvement of no more than one or two amino acid residues and localized tertiary structure conformation changes of the apoprotein must be limited to a very short segment of the protein chain containing only a few aromatic amino acid residues, and (d) the interaction between the apoprotein and retinal seems to be relatively more pronounced when the retinal is in the all-trans form than the 13-cis from and also the apoprotein seems to impose a more pronounced dissymmetric constraint on the retinal in the all-trans form than in the 13-cis form.", "contents": "Effects of light adaptation on the purple membrane structure of Halobacterium halobium. Absorption, circular dichroism and optical rotatory dispersion of the bacteriorhodopsin containing purple membrane form Halobacterium halobium were studied in regard to the structural stability of this membrane during the photoisomerization of the retinal of the bacteriorhodopsin from the 13-cis to the all-trans configuration. The following conclusions were reached: (a) the macromolecular structure (protein-protein interaction which may result in the possible exciton interaction of the retinal pi-pi* (NV1) transition moments and protein-lipid interaction) are not significantly altered, (b) possibilities of delocalized conformation changes of the apoprotein involving secondary and/or tertiary structure can be ruled out, (c) localized secondary structure conformation changes of the apoprotein must be limited to the involvement of no more than one or two amino acid residues and localized tertiary structure conformation changes of the apoprotein must be limited to a very short segment of the protein chain containing only a few aromatic amino acid residues, and (d) the interaction between the apoprotein and retinal seems to be relatively more pronounced when the retinal is in the all-trans form than the 13-cis from and also the apoprotein seems to impose a more pronounced dissymmetric constraint on the retinal in the all-trans form than in the 13-cis form.", "PMID": 963198}
{"id": "pubmed23n0032_5451", "title": "On electron transfer.", "content": "The tunneling transfer of electrons between two sites, appropriate to biological intermolecular electron transfer, contains physical considerations which are not important in the tunneling transfer between two metals. Analyses (such as the recent one by Hales) based on the well-known formula for the latter but applied to the former case are quantitatively and qualitatively misleading.", "contents": "On electron transfer. The tunneling transfer of electrons between two sites, appropriate to biological intermolecular electron transfer, contains physical considerations which are not important in the tunneling transfer between two metals. Analyses (such as the recent one by Hales) based on the well-known formula for the latter but applied to the former case are quantitatively and qualitatively misleading.", "PMID": 963200}
{"id": "pubmed23n0032_5452", "title": "Prediction of the conformation of the histones.", "content": "The secondary structures of the histones, H1, H2A, H2B, H3, and H4 have been predicted utilizing the predictive scheme of Chou and Fasman (Biochemistry 13:211, 222[1974]) and a new set of conformational parameters based on the X-ray data of 29 protein structures. The alpha-helical, beta-sheet, reverse beta-turns, and random coil regions of these proteins are carefully delineated. Structures are specified which are most probably under various environmental conditions, i.e., for changes in ionic strength, association between histones and in association with DNA. Potential conformational changes within these histones are also predicted.", "contents": "Prediction of the conformation of the histones. The secondary structures of the histones, H1, H2A, H2B, H3, and H4 have been predicted utilizing the predictive scheme of Chou and Fasman (Biochemistry 13:211, 222[1974]) and a new set of conformational parameters based on the X-ray data of 29 protein structures. The alpha-helical, beta-sheet, reverse beta-turns, and random coil regions of these proteins are carefully delineated. Structures are specified which are most probably under various environmental conditions, i.e., for changes in ionic strength, association between histones and in association with DNA. Potential conformational changes within these histones are also predicted.", "PMID": 963199}
{"id": "pubmed23n0032_5453", "title": "Fragmentation of chromatin with 125I radioactive disintegrations.", "content": "The DNA in Chinese hamster cells was labeled first for 3 h with [3H]TdR and then for 3 h with [125I]UdR. Chromatin was extracted, frozen, and stored at -30 degrees C until 1.0 X 10(17) and 1.25 X 10(17) disintegrations/g of labeled DNA occurred for 125I and 3H respectively. Velocity sedimentation of chromatin (DNA with associated chromosomal proteins) in neutral sucrose gradients indicated that the localized energy from the 125I disintegrations, which gave about 1 double-strand break/disintegration plus an additional 1.3 single strand breaks, selectively fragmented the [125I] chromatin into pieces smaller than the [3H] chromatin. In other words, 125I disintegrations caused much more localized damage in the chromatin labeled with 125I than in the chromatin labeled with 3H, and fragments induced in DNA by 125I disintegrations were not held together by the associated chromosomal proteins. Use of this 125I technique for studying chromosomal proteins associated with different regions in the cellular DNA is discussed. For these studies, the number of disintegrations required for fragmenting DNA molecules of different sizes is illustrated.", "contents": "Fragmentation of chromatin with 125I radioactive disintegrations. The DNA in Chinese hamster cells was labeled first for 3 h with [3H]TdR and then for 3 h with [125I]UdR. Chromatin was extracted, frozen, and stored at -30 degrees C until 1.0 X 10(17) and 1.25 X 10(17) disintegrations/g of labeled DNA occurred for 125I and 3H respectively. Velocity sedimentation of chromatin (DNA with associated chromosomal proteins) in neutral sucrose gradients indicated that the localized energy from the 125I disintegrations, which gave about 1 double-strand break/disintegration plus an additional 1.3 single strand breaks, selectively fragmented the [125I] chromatin into pieces smaller than the [3H] chromatin. In other words, 125I disintegrations caused much more localized damage in the chromatin labeled with 125I than in the chromatin labeled with 3H, and fragments induced in DNA by 125I disintegrations were not held together by the associated chromosomal proteins. Use of this 125I technique for studying chromosomal proteins associated with different regions in the cellular DNA is discussed. For these studies, the number of disintegrations required for fragmenting DNA molecules of different sizes is illustrated.", "PMID": 963201}
{"id": "pubmed23n0032_5454", "title": "Computer simulation of movement-generating cross-bridges.", "content": "A stochastic computational method was developed to study properties of cross-bridge models for muscle contraction, by following the time history of individual cross-bridge model of Andrew Huxley (1957) and a modified two-state model with more realistic behavior during steady stretching are used as examples. The method can readily compute steady-state force during shortening and stretching and force-transients following rapid changes in length. Computations of velocity with a steady load and of velocity transients are more sensitive to the randomness inherent in the stochastic method.", "contents": "Computer simulation of movement-generating cross-bridges. A stochastic computational method was developed to study properties of cross-bridge models for muscle contraction, by following the time history of individual cross-bridge model of Andrew Huxley (1957) and a modified two-state model with more realistic behavior during steady stretching are used as examples. The method can readily compute steady-state force during shortening and stretching and force-transients following rapid changes in length. Computations of velocity with a steady load and of velocity transients are more sensitive to the randomness inherent in the stochastic method.", "PMID": 963202}
{"id": "pubmed23n0032_5455", "title": "Computer simulation of flagellar movement. IV. Properties of an oscillatory two-state cross-bridge model.", "content": "A stochastic computational method is used to examine the properties of a simple two-state cross-bridge model, of a type which has been shown previously to self-oscillate without requiring any feedback control of the active process. The force transients obtained with this model show the major features observed with oscillatory insect fibrillar flight muscle. The effects of viscosity and cross-bridge detachment rate on the frequency of oscillation of the model resemble the effects of viscosity and ATP concentration on flagellar oscillation, and the relationship between turnover rate and frequency of oscillation is also consistent with observations on flagella. However, the amplitude of oscillation of the model does not show the degree of frequency-independence which is typical of flagella.", "contents": "Computer simulation of flagellar movement. IV. Properties of an oscillatory two-state cross-bridge model. A stochastic computational method is used to examine the properties of a simple two-state cross-bridge model, of a type which has been shown previously to self-oscillate without requiring any feedback control of the active process. The force transients obtained with this model show the major features observed with oscillatory insect fibrillar flight muscle. The effects of viscosity and cross-bridge detachment rate on the frequency of oscillation of the model resemble the effects of viscosity and ATP concentration on flagellar oscillation, and the relationship between turnover rate and frequency of oscillation is also consistent with observations on flagella. However, the amplitude of oscillation of the model does not show the degree of frequency-independence which is typical of flagella.", "PMID": 963203}
{"id": "pubmed23n0032_5456", "title": "Nuclear magnetic resonance measurement of skeletal muscle: anisotrophy of the diffusion coefficient of the intracellular water.", "content": "The anisotropy of the spin-diffusion coefficient Ds of water protons in skeletal muscle has been studied by pulsed NMR methods. The mid-portion of the tibialis anterior muscle of mature male rats was placed in a special sample holder by means of which the muscle fiber orientation theta relative to the diffusion direction could be varied over the range 0 degrees less than or equal to theta less than or equal to 90 degrees. The value of Ds(theta) was determined for theta = 0 degrees, 45 degrees, and 90 degrees. The measured anisotropy Ds(0)/Ds(90) was 1.39, and the value of Ds(0) was 1.39 X 10(-5) cm2/s. These results are interpreted within the framework of a model calculation in which the diffusion equation is solved for a regular hexagonal network similar to the actin-myosin filament network. The large anisotropy, and the large reduction in the value of Ds measured parallel to the filament axes lead to two major conclusions: (a) interpretations in which the reduction in Ds is ascribed to the effect of geometrical obstructions on the diffusion of \"free\" water are ruled out; and, (b) there is a large fraction of the cellular water associated with the proteins in such a way that its diffusion coefficient is substantially reduced.", "contents": "Nuclear magnetic resonance measurement of skeletal muscle: anisotrophy of the diffusion coefficient of the intracellular water. The anisotropy of the spin-diffusion coefficient Ds of water protons in skeletal muscle has been studied by pulsed NMR methods. The mid-portion of the tibialis anterior muscle of mature male rats was placed in a special sample holder by means of which the muscle fiber orientation theta relative to the diffusion direction could be varied over the range 0 degrees less than or equal to theta less than or equal to 90 degrees. The value of Ds(theta) was determined for theta = 0 degrees, 45 degrees, and 90 degrees. The measured anisotropy Ds(0)/Ds(90) was 1.39, and the value of Ds(0) was 1.39 X 10(-5) cm2/s. These results are interpreted within the framework of a model calculation in which the diffusion equation is solved for a regular hexagonal network similar to the actin-myosin filament network. The large anisotropy, and the large reduction in the value of Ds measured parallel to the filament axes lead to two major conclusions: (a) interpretations in which the reduction in Ds is ascribed to the effect of geometrical obstructions on the diffusion of \"free\" water are ruled out; and, (b) there is a large fraction of the cellular water associated with the proteins in such a way that its diffusion coefficient is substantially reduced.", "PMID": 963204}
{"id": "pubmed23n0032_5457", "title": "Relaxation phenomena in human erythrocyte suspensions.", "content": "Previous work has shown that the application of the Joule heating temperature jump technique of Eigen and de Maeyer to an istonic suspension of human erythrocytes induced an interiorization of [3H-A1glucose and a hemolysis of the red cells (Tsong, T.Y., and E. Kingsley, J. Biol. Chem. 250:786 [1975]). The result was interpreted as due to the thermal osmosis effect. Further considerations of the various effects of the Joule heating technique indicate that the hemolysis of the red cells may also be caused by the rapid dielectric perturbation of the cell membranes. By means of turbidity measurements of the suspensions we have detected at least four relaxation times. Two of the faster ones (tau1 approximately 20 mus and tau2 approximately 5 ms) are tentatively attributed to water relaxations in the membrane structures. The other two are attributed to membrane ruptures (tlag approximately 0.1s) and the hemolysis reaction (tau3 approximately 0.5 s). Studies with the erythrocytes from different hematological disorders indicate that whereas the two slower relaxations are sensitive to the overall physical property of the red cell membranes the two faster relaxations are not. These observations are consistent with the above assignment of the relaxation processes. The apparent activation energies are, above assignment of the relaxation processes. The apparent activation energies are, respectively, 8.4, 12.0, and 11.8 kcal/mol for the tau1, tau2, and tau3 reactions. Experiments with erythrocyte ghosts indicate a single relaxation for the water permeation, and biphasic kinetics for the membrane rupture and resealing reactions. The phenomena reported here may contribute to our understanding of water transport and molecular release in cellular systems.", "contents": "Relaxation phenomena in human erythrocyte suspensions. Previous work has shown that the application of the Joule heating temperature jump technique of Eigen and de Maeyer to an istonic suspension of human erythrocytes induced an interiorization of [3H-A1glucose and a hemolysis of the red cells (Tsong, T.Y., and E. Kingsley, J. Biol. Chem. 250:786 [1975]). The result was interpreted as due to the thermal osmosis effect. Further considerations of the various effects of the Joule heating technique indicate that the hemolysis of the red cells may also be caused by the rapid dielectric perturbation of the cell membranes. By means of turbidity measurements of the suspensions we have detected at least four relaxation times. Two of the faster ones (tau1 approximately 20 mus and tau2 approximately 5 ms) are tentatively attributed to water relaxations in the membrane structures. The other two are attributed to membrane ruptures (tlag approximately 0.1s) and the hemolysis reaction (tau3 approximately 0.5 s). Studies with the erythrocytes from different hematological disorders indicate that whereas the two slower relaxations are sensitive to the overall physical property of the red cell membranes the two faster relaxations are not. These observations are consistent with the above assignment of the relaxation processes. The apparent activation energies are, above assignment of the relaxation processes. The apparent activation energies are, respectively, 8.4, 12.0, and 11.8 kcal/mol for the tau1, tau2, and tau3 reactions. Experiments with erythrocyte ghosts indicate a single relaxation for the water permeation, and biphasic kinetics for the membrane rupture and resealing reactions. The phenomena reported here may contribute to our understanding of water transport and molecular release in cellular systems.", "PMID": 963205}
{"id": "pubmed23n0032_5458", "title": "Models for 1/f noise in nerve membranes.", "content": "A recently proposed model for 1/f(w-1) noise in nerve membrane (Clay and Schlesinger, 1976; Lundstr\u00f6m and McQueen, 1974) is shown to be mathematically inconsistent in several respects. A self-consistent model based on similar membranes lipid orientation fluctuation effects is proposed.", "contents": "Models for 1/f noise in nerve membranes. A recently proposed model for 1/f(w-1) noise in nerve membrane (Clay and Schlesinger, 1976; Lundstr\u00f6m and McQueen, 1974) is shown to be mathematically inconsistent in several respects. A self-consistent model based on similar membranes lipid orientation fluctuation effects is proposed.", "PMID": 963206}
{"id": "pubmed23n0032_5459", "title": "Branched bimolecular lipid membranes.", "content": "Branched bimolecular lipid membranes separating three and four aqueous phases have been formed. The procedure is based on the technique of Montal and Mueller generalized to three and four lipid surface films spanning an appropriate aperture. The technique to produce Teflon structures for the mechanical support of branched bilayers is presented. The existence of the branched bilayer was established by measuring the specific capacity, specific resistance, and the gramicidin-induced single channel conductance of each branch. These structures should facilitate the study of transport properties of ionophores and other molecules and may also serve as model systems for the study of cell fusion.", "contents": "Branched bimolecular lipid membranes. Branched bimolecular lipid membranes separating three and four aqueous phases have been formed. The procedure is based on the technique of Montal and Mueller generalized to three and four lipid surface films spanning an appropriate aperture. The technique to produce Teflon structures for the mechanical support of branched bilayers is presented. The existence of the branched bilayer was established by measuring the specific capacity, specific resistance, and the gramicidin-induced single channel conductance of each branch. These structures should facilitate the study of transport properties of ionophores and other molecules and may also serve as model systems for the study of cell fusion.", "PMID": 963207}
{"id": "pubmed23n0032_5460", "title": "Laser light-scattering spectroscopy: a new application in the study of ciliary activity.", "content": "A uniquely precise and simple method to study ciliary activity by laser light-scattering spectroscopy has been developed and validated. A concurrent study of the effect of Ca2+ on ciliary activity in vitro by laser scattering spectroscopy and high speed cinematography has demonstrated that this new method is simpler and as accurate and reproducible as the high speed film technique.", "contents": "Laser light-scattering spectroscopy: a new application in the study of ciliary activity. A uniquely precise and simple method to study ciliary activity by laser light-scattering spectroscopy has been developed and validated. A concurrent study of the effect of Ca2+ on ciliary activity in vitro by laser scattering spectroscopy and high speed cinematography has demonstrated that this new method is simpler and as accurate and reproducible as the high speed film technique.", "PMID": 963208}
{"id": "pubmed23n0032_5461", "title": "Exact solution of the unidimensional Poisson-Boltzmann equation for a 1:2 (2:1) electrolyte.", "content": "The unidimensional Poisson-Boltzmann equation for a 1:2 (2:1) electrolyte has been solved analytically. The results have been compared with those obtained from the linearized equation. It is shown that in physiological conditions the difference may be greater than 10%. The value of the derivative of the potential in x=0, (dpsi/dx)x=0, has been used by many authors in the evaluation of the superficial charges of biological membranes. The value of (dpsi/dx)x-0 have also been compared with the ones derived from the linearized equation. The difference may be greater than 25%. Our results suggest that the linearization of the Poisson-Boltzmann equation for a 1:2(2:1) electrolyte may be greatly misleading.", "contents": "Exact solution of the unidimensional Poisson-Boltzmann equation for a 1:2 (2:1) electrolyte. The unidimensional Poisson-Boltzmann equation for a 1:2 (2:1) electrolyte has been solved analytically. The results have been compared with those obtained from the linearized equation. It is shown that in physiological conditions the difference may be greater than 10%. The value of the derivative of the potential in x=0, (dpsi/dx)x=0, has been used by many authors in the evaluation of the superficial charges of biological membranes. The value of (dpsi/dx)x-0 have also been compared with the ones derived from the linearized equation. The difference may be greater than 25%. Our results suggest that the linearization of the Poisson-Boltzmann equation for a 1:2(2:1) electrolyte may be greatly misleading.", "PMID": 963209}
{"id": "pubmed23n0032_5462", "title": "Activation-inactivation coupling in Myxicola giant axons injected with tetraethylammonium.", "content": "Myxicola giant axons internally injected with tetraethylammonium chloride to block potassium currents were examined under voltage clamp. The sodium inactivation time constants obtained from the decline in INa during step depolarizations were substantially smaller than those obtained using conditioning prepulses to the same potentials and the ratios agreed with previous observations using TTX. Inactivation shifts were also measured and found to be comparable to previous results.", "contents": "Activation-inactivation coupling in Myxicola giant axons injected with tetraethylammonium. Myxicola giant axons internally injected with tetraethylammonium chloride to block potassium currents were examined under voltage clamp. The sodium inactivation time constants obtained from the decline in INa during step depolarizations were substantially smaller than those obtained using conditioning prepulses to the same potentials and the ratios agreed with previous observations using TTX. Inactivation shifts were also measured and found to be comparable to previous results.", "PMID": 963210}
{"id": "pubmed23n0032_5463", "title": "The electrical resistivity of cytoplasm.", "content": "The apparent cytoplasmic resistivity of two different giant cells has been measured using an extension of a previously developed single microelectrode technique. Each cell is penetrated by a metal microelectrode whose complex impedance is measured as a function of frequency between 500 kHz and 5.7 MHz. By plotting the measured impedance data on the complex Z plane and extrapolating the data to infinite frequency, the substantial effects of electrode polarization can be overcome. For Aplysia giant neurons and muscle fibers of the giant barnacle, the extrapolated cytoplasmic specific resistivities are 40 and 74 omega-cm, respectively, at infinite frequency. The barnacle data are in excellent agreement with sarcoplasmic resistivity values derived from the measured cable properties of other marine organisms, and from high frequency conductivity cell measurements in intact barnacle muscle tissue. In the Aplysia neurons, the frequency-dependent part of the electrode impedance is larger when the electrode is in a cell than when it is in an electrolyte solution with the same specific resistivity as the aqueous cytoplasm; however, the phase angle of the frequency-dependent component of the electrode impedance is the same in both cases. This suggests that the high apparent values of cytoplasmic resistivity found using the single microelectrode technique at lower frequencies probably reflect an artifact caused by reduction of the effective surface area of the electrode by intracellular membranes, with a corresponding increase in its polarization impedance.", "contents": "The electrical resistivity of cytoplasm. The apparent cytoplasmic resistivity of two different giant cells has been measured using an extension of a previously developed single microelectrode technique. Each cell is penetrated by a metal microelectrode whose complex impedance is measured as a function of frequency between 500 kHz and 5.7 MHz. By plotting the measured impedance data on the complex Z plane and extrapolating the data to infinite frequency, the substantial effects of electrode polarization can be overcome. For Aplysia giant neurons and muscle fibers of the giant barnacle, the extrapolated cytoplasmic specific resistivities are 40 and 74 omega-cm, respectively, at infinite frequency. The barnacle data are in excellent agreement with sarcoplasmic resistivity values derived from the measured cable properties of other marine organisms, and from high frequency conductivity cell measurements in intact barnacle muscle tissue. In the Aplysia neurons, the frequency-dependent part of the electrode impedance is larger when the electrode is in a cell than when it is in an electrolyte solution with the same specific resistivity as the aqueous cytoplasm; however, the phase angle of the frequency-dependent component of the electrode impedance is the same in both cases. This suggests that the high apparent values of cytoplasmic resistivity found using the single microelectrode technique at lower frequencies probably reflect an artifact caused by reduction of the effective surface area of the electrode by intracellular membranes, with a corresponding increase in its polarization impedance.", "PMID": 963211}
{"id": "pubmed23n0032_5464", "title": "Volume of reaction by the Archibald ultracentrifuge method (lobster hemocyanin).", "content": "Samples of lobster hemocyanin (Homarus americanus) under conditions of reversible reaction between whole (25 S) and half (17 S) molecules have been subjected to accurately known nitrogen pressures in analytical ultracentrifuge cells. A modified pressurization chamber of the type developed by Schumaker and colleagues has been constructed for this purpose. The molecular weight was then determined at the top (liquid-gas) meniscus, by means of the Archibald method. The logarithmic dependence upon pressure of the derived equilibrium constant then gave directly the volume of reaction. Experiments were performed in veronal-citrate buffers at pH 8, where the molar volume of formation of whole (dodecameric) molecules from half molecules appears to be negative, and at pH 8.46 in veronal-citrate buffer in the presence of 0.003 molar free calcium ion, where the molar volume of formation was estimated to be + 390 cm3/mole. In glycine-sodium hydroxide buffer at pH 9.6 containing 0.0047 molar free calcium, the molar volume of formation of whole molecules was estimated to be +120 +/- 70 cm3, corresponding to an estimated difference in partial specific volume between whole molecules and half molecules of only 1.3 (10)-4cm3/gram. The correctness of the sign of this value in glycine buffer has been verified by pressure-jump light-scattering experiments.", "contents": "Volume of reaction by the Archibald ultracentrifuge method (lobster hemocyanin). Samples of lobster hemocyanin (Homarus americanus) under conditions of reversible reaction between whole (25 S) and half (17 S) molecules have been subjected to accurately known nitrogen pressures in analytical ultracentrifuge cells. A modified pressurization chamber of the type developed by Schumaker and colleagues has been constructed for this purpose. The molecular weight was then determined at the top (liquid-gas) meniscus, by means of the Archibald method. The logarithmic dependence upon pressure of the derived equilibrium constant then gave directly the volume of reaction. Experiments were performed in veronal-citrate buffers at pH 8, where the molar volume of formation of whole (dodecameric) molecules from half molecules appears to be negative, and at pH 8.46 in veronal-citrate buffer in the presence of 0.003 molar free calcium ion, where the molar volume of formation was estimated to be + 390 cm3/mole. In glycine-sodium hydroxide buffer at pH 9.6 containing 0.0047 molar free calcium, the molar volume of formation of whole molecules was estimated to be +120 +/- 70 cm3, corresponding to an estimated difference in partial specific volume between whole molecules and half molecules of only 1.3 (10)-4cm3/gram. The correctness of the sign of this value in glycine buffer has been verified by pressure-jump light-scattering experiments.", "PMID": 963212}
{"id": "pubmed23n0032_5465", "title": "Simulation studies of self-associating systems; discrimination between specific and isodesmic associations.", "content": "The possibilities of discriminating between definite and indefinite (isodesmic) modes of self-association are explored by fitting the different models to stimulated data, using non-linear least-squares curve-fitting to determine the fitting parameters for real and impostor models. It was found that over an extensive range of values for the equilibrium constant of a non-ideal isodesmic generating model, only a non-ideal monomer-dimer-tetramer-octamer was a successful impostor model. Some criteria for rejecting inappropriate models are discussed.", "contents": "Simulation studies of self-associating systems; discrimination between specific and isodesmic associations. The possibilities of discriminating between definite and indefinite (isodesmic) modes of self-association are explored by fitting the different models to stimulated data, using non-linear least-squares curve-fitting to determine the fitting parameters for real and impostor models. It was found that over an extensive range of values for the equilibrium constant of a non-ideal isodesmic generating model, only a non-ideal monomer-dimer-tetramer-octamer was a successful impostor model. Some criteria for rejecting inappropriate models are discussed.", "PMID": 963213}
{"id": "pubmed23n0032_5466", "title": "Sedimentation equilibrium techniques: multiple speed analyses and an overspeed procedure.", "content": "The use of a single high-speed sedimentation equilibrium experiment to analyze mixed associating systems is inadequate to determine the association mode even if the molecular weight of one species is known. Simultaneous analysis of the concentration distributions at three equilibrium speeds greatly reduces the lack of uniqueness. Linear least-squares multi-speed fits discriminate between association models in which the molecular weights are assumed. Experiments at a series of initial concentrations as well as rotor speeds further increases the discrimination. An overspeed procedure is proposed. The overspeed time depends only slightly on the sedimenting species molecular weight, but significantly on the frictional ratio. A minimal overspeed time may be estimated.", "contents": "Sedimentation equilibrium techniques: multiple speed analyses and an overspeed procedure. The use of a single high-speed sedimentation equilibrium experiment to analyze mixed associating systems is inadequate to determine the association mode even if the molecular weight of one species is known. Simultaneous analysis of the concentration distributions at three equilibrium speeds greatly reduces the lack of uniqueness. Linear least-squares multi-speed fits discriminate between association models in which the molecular weights are assumed. Experiments at a series of initial concentrations as well as rotor speeds further increases the discrimination. An overspeed procedure is proposed. The overspeed time depends only slightly on the sedimenting species molecular weight, but significantly on the frictional ratio. A minimal overspeed time may be estimated.", "PMID": 963214}
{"id": "pubmed23n0032_5467", "title": "Improvement in precision of sedimentation-equilibrium experiments with an on-line absorption scanner.", "content": "An on-line computerized optical absorption scanner for the analytical ultracentrifuge has been developed and tested. It makes direct use of the instantaneous photomultiplier output, which is digitized and averaged by the computer. It incorporates a stepping motor to drive the photomultiplier, two analog-to-digital converters, and a device which monitors precessional movement of the rotor. The scanner has been employed chiefly in sedimentation equilibrium experiments, and techniques have been devised to correct for the adverse effects of inhomogeneities of apparent absorbance which arise from the cell windows. Correction for rotor movements which occur during the scan is also possible. The completed apparatus is a successful one, and provides a significant improvement in precision, speed and convenience over the commercially available scanners. The limitations on precision inherent in the present design are examined, and it is concluded that the combined effects of window inhomogeneities and rotor movement lead to uncertainty of the measured absorbance which is unlikely to be reduced below +/- 0.001 A.", "contents": "Improvement in precision of sedimentation-equilibrium experiments with an on-line absorption scanner. An on-line computerized optical absorption scanner for the analytical ultracentrifuge has been developed and tested. It makes direct use of the instantaneous photomultiplier output, which is digitized and averaged by the computer. It incorporates a stepping motor to drive the photomultiplier, two analog-to-digital converters, and a device which monitors precessional movement of the rotor. The scanner has been employed chiefly in sedimentation equilibrium experiments, and techniques have been devised to correct for the adverse effects of inhomogeneities of apparent absorbance which arise from the cell windows. Correction for rotor movements which occur during the scan is also possible. The completed apparatus is a successful one, and provides a significant improvement in precision, speed and convenience over the commercially available scanners. The limitations on precision inherent in the present design are examined, and it is concluded that the combined effects of window inhomogeneities and rotor movement lead to uncertainty of the measured absorbance which is unlikely to be reduced below +/- 0.001 A.", "PMID": 963215}
{"id": "pubmed23n0032_5468", "title": "Molecular weights and molecular-weight distributions from ultracentrifugation of nonideal solutions.", "content": "Ultracentrifugation, membrane osmometry and capillary viscometry experiments have been performed on two dextran samples, which have molecular-weight distributions (MWDs) similar to those of dextrans used as blood plasma extenders. The manufacturer reported values of Mn and MW, determined by end group analysis and by light scattering, respectively. Our values of Mn, determined by osmometry, and MW, calculated from ultracentrifugal and viscometry experiments, agreed quite well with the manufacturer's results. Good agreement was obtained with values of MW and BLS (the light scattering second virial coefficient) obtained from sedimentation equilibrium experiments at different speeds using sector or nonsector-shaped centerpieces. Several ways of obtaining MW, MZ and BLS from sedimentation equilibrium experiments are presented. We have also shown how to obtain the speed-dependent term of the sedimentation equilibrium second virial coefficient. Both BLS and the speed-dependent nonideal terms could be used to correct the sedimentation equilibrium data, so that ideal values of d in c/d(r2) or dc/d(r2) could be estimated and used to obtain the MWDs of the dextran samples. Both Donnelly's and Scholte's methods were used with the sedimentation equilibrium data. With both methods, unimodal MWDs were encountered, which gave good agreement with the manufacturer's MWDs, obtained by a combination of analytical gel chromatography and light scattering. Uncorrected sedimentation equilibrium data gave MWDs quite different from the manufacturer's results. The MWD calculated from the differential distribution of sedimentation coefficients also gave a unimodal MWD, but this MWD did not give a good agreement with the sedimentation equilibrium results or with the manufacturer's results.", "contents": "Molecular weights and molecular-weight distributions from ultracentrifugation of nonideal solutions. Ultracentrifugation, membrane osmometry and capillary viscometry experiments have been performed on two dextran samples, which have molecular-weight distributions (MWDs) similar to those of dextrans used as blood plasma extenders. The manufacturer reported values of Mn and MW, determined by end group analysis and by light scattering, respectively. Our values of Mn, determined by osmometry, and MW, calculated from ultracentrifugal and viscometry experiments, agreed quite well with the manufacturer's results. Good agreement was obtained with values of MW and BLS (the light scattering second virial coefficient) obtained from sedimentation equilibrium experiments at different speeds using sector or nonsector-shaped centerpieces. Several ways of obtaining MW, MZ and BLS from sedimentation equilibrium experiments are presented. We have also shown how to obtain the speed-dependent term of the sedimentation equilibrium second virial coefficient. Both BLS and the speed-dependent nonideal terms could be used to correct the sedimentation equilibrium data, so that ideal values of d in c/d(r2) or dc/d(r2) could be estimated and used to obtain the MWDs of the dextran samples. Both Donnelly's and Scholte's methods were used with the sedimentation equilibrium data. With both methods, unimodal MWDs were encountered, which gave good agreement with the manufacturer's MWDs, obtained by a combination of analytical gel chromatography and light scattering. Uncorrected sedimentation equilibrium data gave MWDs quite different from the manufacturer's results. The MWD calculated from the differential distribution of sedimentation coefficients also gave a unimodal MWD, but this MWD did not give a good agreement with the sedimentation equilibrium results or with the manufacturer's results.", "PMID": 963217}
{"id": "pubmed23n0032_5469", "title": "Sedimentation in the ultracentrifuge and diffusion of macromolecules carrying electrical charges.", "content": "The calculation of Alexandrowicz and Daniel of the charge effect in the sedimentation of macromolecules carrying electrical charges was reformulated by introducing buoyant molecular weights and concentrations of electroneutral components only (rather than those of the ionized species) in the final equations. Some interesting limiting cases are discussed. A similar calculation was performed for the case of isothermal diffusion. It is also shown how the present discussion relates to more elaborate treatments of the sedimentation and diffusion of polyelectrolytes by the methods of irreversible thermodynamics.", "contents": "Sedimentation in the ultracentrifuge and diffusion of macromolecules carrying electrical charges. The calculation of Alexandrowicz and Daniel of the charge effect in the sedimentation of macromolecules carrying electrical charges was reformulated by introducing buoyant molecular weights and concentrations of electroneutral components only (rather than those of the ionized species) in the final equations. Some interesting limiting cases are discussed. A similar calculation was performed for the case of isothermal diffusion. It is also shown how the present discussion relates to more elaborate treatments of the sedimentation and diffusion of polyelectrolytes by the methods of irreversible thermodynamics.", "PMID": 963218}
{"id": "pubmed23n0032_5470", "title": "Numerical study of the Johnston-Ogston effect in two-component systems.", "content": "Numerical solutions of the Lamm equation are presented for systems exhibiting the Johnston--Ogston effect. From these solutions it is apparent that the movement of the maxima of the concentration gradient curves reflects the sedimentation velocity of the slow or fast components in their appropriate plateaus. A simple generalization of the Johnston--Ogston analysis is presented, valid for all centrifugation times in a radial field and sector shaped cell provided only that there exist both a plateau of the slow component by itself and the mixed plateau with both slow and fast components present.", "contents": "Numerical study of the Johnston-Ogston effect in two-component systems. Numerical solutions of the Lamm equation are presented for systems exhibiting the Johnston--Ogston effect. From these solutions it is apparent that the movement of the maxima of the concentration gradient curves reflects the sedimentation velocity of the slow or fast components in their appropriate plateaus. A simple generalization of the Johnston--Ogston analysis is presented, valid for all centrifugation times in a radial field and sector shaped cell provided only that there exist both a plateau of the slow component by itself and the mixed plateau with both slow and fast components present.", "PMID": 963219}
{"id": "pubmed23n0032_5471", "title": "Anomalies in sedimentation. V. Chains at high fields, practical consequences.", "content": "The theory of a preceding paper [B.H. Zimm, Biophys, Chem. 1 (1974) 279] is used to calculate a numerical table for the change of sedimentation coefficient with centrifugal field for chain molecules. A simple formula is found to fit the results within 1.3% up to the centrifugal field at which S/So = 0.377; this formula is S/So=(1+0.1155y2)-1/4, where y is proportional to M2/So times the centripetal acceleration, M being the molecular weight and So the sedimentation coefficient at zero acceleration. Applying this formula to DNA, we conclude that at a given centrifuge speed the sedimentation coefficient must reach a maximum at a particular molecular weight and be smaller at higher molecular weights. The value of the maximum depends on the conditions, but can come at less than 150 S for DNA under typical conditions. When a maximum is present, the profile of a sedimenting non-homogeneous band is also severely distorted.", "contents": "Anomalies in sedimentation. V. Chains at high fields, practical consequences. The theory of a preceding paper [B.H. Zimm, Biophys, Chem. 1 (1974) 279] is used to calculate a numerical table for the change of sedimentation coefficient with centrifugal field for chain molecules. A simple formula is found to fit the results within 1.3% up to the centrifugal field at which S/So = 0.377; this formula is S/So=(1+0.1155y2)-1/4, where y is proportional to M2/So times the centripetal acceleration, M being the molecular weight and So the sedimentation coefficient at zero acceleration. Applying this formula to DNA, we conclude that at a given centrifuge speed the sedimentation coefficient must reach a maximum at a particular molecular weight and be smaller at higher molecular weights. The value of the maximum depends on the conditions, but can come at less than 150 S for DNA under typical conditions. When a maximum is present, the profile of a sedimenting non-homogeneous band is also severely distorted.", "PMID": 963220}
{"id": "pubmed23n0032_5472", "title": "UV laser scanning and fluorescence monitoring of analytical ultracentrifugation with an on-line computer system.", "content": "A new optical system for the analytical ultracentrifuge is described, which permits sedimentation to be monitored by fluorescence. The optical system is based on a laser light source, which is focused to a narrow (50 micron) beam. The radical scanning of the beam provides information on the distribution of fluorescing material with distance in the centrifuge cell. Data collection and processing are performed in conjunction with an on-line computer system which sorts incoming fluorescence pulses according to rotor hole and cell sector, averages families of pulses to improve signal to noise ratios and fits the data (in the experiments reported here) to equations to determine sedimentation coefficients. Initial experiments with the system have been performed with bovine serum albumin and indicate that sedimentation can be readily monitored by fluorescence with solutions at concentrations as low as 20 micrograms per ml, with excitation at 257 nm. At these concentrations, the optical density is only in the 0.01 range, too low for experiments with absorption-scanner optical system. Even lower concentrations can be used when fluorescent labels are used with excitation in the visible region of the spectrum. The preliminary studies indicate that fluorescence monitoring of sedimentation will substantially enhance the range of experimental possibilities in ultracentrifugation by improving both the sensitivity of measurements and the discrimination between sedimenting species on the basis of their fluorescence characteristics.", "contents": "UV laser scanning and fluorescence monitoring of analytical ultracentrifugation with an on-line computer system. A new optical system for the analytical ultracentrifuge is described, which permits sedimentation to be monitored by fluorescence. The optical system is based on a laser light source, which is focused to a narrow (50 micron) beam. The radical scanning of the beam provides information on the distribution of fluorescing material with distance in the centrifuge cell. Data collection and processing are performed in conjunction with an on-line computer system which sorts incoming fluorescence pulses according to rotor hole and cell sector, averages families of pulses to improve signal to noise ratios and fits the data (in the experiments reported here) to equations to determine sedimentation coefficients. Initial experiments with the system have been performed with bovine serum albumin and indicate that sedimentation can be readily monitored by fluorescence with solutions at concentrations as low as 20 micrograms per ml, with excitation at 257 nm. At these concentrations, the optical density is only in the 0.01 range, too low for experiments with absorption-scanner optical system. Even lower concentrations can be used when fluorescent labels are used with excitation in the visible region of the spectrum. The preliminary studies indicate that fluorescence monitoring of sedimentation will substantially enhance the range of experimental possibilities in ultracentrifugation by improving both the sensitivity of measurements and the discrimination between sedimenting species on the basis of their fluorescence characteristics.", "PMID": 963221}
{"id": "pubmed23n0032_5473", "title": "Collecting and processing records from the ultracentrifuge in \"real-time\" using an on-line computer.", "content": "An analytical system consisting of an analytical cantrifuge coupled 'on-line' to a computer was assembled and tested. Collection of records from up to 9 solutions was achieved through programmes which sum readings to reduce noise as well as controlling the positioning of the scanner. With this system it was found that the limit on accuracy for molecular weights at concentrations less than 0.01 g cm-3 was +/- 3% estimated from sedimentation equilibrium experiments. The same system was used to collect records for similar concentrations from velocity experiments by employing a scanning schlieren. In this case the accuracy in estimating sedimentation coefficients was similar to those found when measuring photographs. Since the collection yields detailed information about the shape of the sedimenting boundary, the centroids of the boundary were routinely computed by second moment analysis rather than relying on the position of the maximum of the schlieren peak. In the same analysis estimates of diffusion coefficients were made routinely by calculating corrected height/area ratios for each scan. These calculations were made during the real-time of the experiment, so making available molecular parameters rather than records which must be evaluated some time after stopping the experiment.", "contents": "Collecting and processing records from the ultracentrifuge in \"real-time\" using an on-line computer. An analytical system consisting of an analytical cantrifuge coupled 'on-line' to a computer was assembled and tested. Collection of records from up to 9 solutions was achieved through programmes which sum readings to reduce noise as well as controlling the positioning of the scanner. With this system it was found that the limit on accuracy for molecular weights at concentrations less than 0.01 g cm-3 was +/- 3% estimated from sedimentation equilibrium experiments. The same system was used to collect records for similar concentrations from velocity experiments by employing a scanning schlieren. In this case the accuracy in estimating sedimentation coefficients was similar to those found when measuring photographs. Since the collection yields detailed information about the shape of the sedimenting boundary, the centroids of the boundary were routinely computed by second moment analysis rather than relying on the position of the maximum of the schlieren peak. In the same analysis estimates of diffusion coefficients were made routinely by calculating corrected height/area ratios for each scan. These calculations were made during the real-time of the experiment, so making available molecular parameters rather than records which must be evaluated some time after stopping the experiment.", "PMID": 963222}
{"id": "pubmed23n0032_5474", "title": "A television scanner for the ultracentrifuge. II. Multiple cell operation.", "content": "The \"Optical Multichannel Analyzer\" (OMA) is a commercially available instrument that with the absorption optical system of the ultracentrifuge, provides an entire 500 channel intensity profile of a cell in real time. With its own analog-todigital converter, the OMA integrates a selectable number of 32.8 msec scans to provide a time-averaged image in digital form. This paper describes an interface-controller for operation of the OMA with single- and double-sector cells in multi-cell rotors, simulating double-beam measurement required for absorbance determinations. The desired sector is selected by \"gating\" the intensifier stage of a \"Silicon Intensified Target\" vidicon (SIT) used as the light detector. The cell location in the rotor and the position of the gate relative to the cell centerline is obtained from a phase-locked loop circuit which divides each rotation of the rotor into 3600 parts independent of rotor speed. (This circuit employed with photo-multiplier scanners would select the gate position for integration of photomultiplier pulses.) From examination of appropriate signals with an oscilloscope, it was verified that gate positions and widths are located with an accuracy of 0.1degree or better and with a precision of +/- 0.1 mus. The light intensity profile for any desired cell can be examined in \"real time\", even during acceleration of the rotor. Additional circuits employing a 10 MHz crystal clock 1) control the automatic collection of data for all sectors in multicell rotors at digitally selected time intervals, 2) display the rotor speed, and 3) indicate the elapsed time of the experiment. Constructed but not tested are additional circuits for pulsing a laser into the absorption or Rayleigh optical system. The accuracy of the pulsed SIT has been demonstrated by measurement of absorbances of solutions and also by sedimentation equilibrium experiments with myoglobin. The estimated error is 0.003 for absorbances ranging from 0 to 1. The interface-controller operates extremely well, but problems related to the pulsed SIT (optimum gate position relative to the sector opening shape of high-voltage pulse, slight pincushion distortion) require more work.", "contents": "A television scanner for the ultracentrifuge. II. Multiple cell operation. The \"Optical Multichannel Analyzer\" (OMA) is a commercially available instrument that with the absorption optical system of the ultracentrifuge, provides an entire 500 channel intensity profile of a cell in real time. With its own analog-todigital converter, the OMA integrates a selectable number of 32.8 msec scans to provide a time-averaged image in digital form. This paper describes an interface-controller for operation of the OMA with single- and double-sector cells in multi-cell rotors, simulating double-beam measurement required for absorbance determinations. The desired sector is selected by \"gating\" the intensifier stage of a \"Silicon Intensified Target\" vidicon (SIT) used as the light detector. The cell location in the rotor and the position of the gate relative to the cell centerline is obtained from a phase-locked loop circuit which divides each rotation of the rotor into 3600 parts independent of rotor speed. (This circuit employed with photo-multiplier scanners would select the gate position for integration of photomultiplier pulses.) From examination of appropriate signals with an oscilloscope, it was verified that gate positions and widths are located with an accuracy of 0.1degree or better and with a precision of +/- 0.1 mus. The light intensity profile for any desired cell can be examined in \"real time\", even during acceleration of the rotor. Additional circuits employing a 10 MHz crystal clock 1) control the automatic collection of data for all sectors in multicell rotors at digitally selected time intervals, 2) display the rotor speed, and 3) indicate the elapsed time of the experiment. Constructed but not tested are additional circuits for pulsing a laser into the absorption or Rayleigh optical system. The accuracy of the pulsed SIT has been demonstrated by measurement of absorbances of solutions and also by sedimentation equilibrium experiments with myoglobin. The estimated error is 0.003 for absorbances ranging from 0 to 1. The interface-controller operates extremely well, but problems related to the pulsed SIT (optimum gate position relative to the sector opening shape of high-voltage pulse, slight pincushion distortion) require more work.", "PMID": 963223}
{"id": "pubmed23n0032_5475", "title": "MaD, an automated precise analytical ultracentrifuge scanner system.", "content": "The various hardware components of this scanning system, a mechanical split-beam scanner with its photomultiplier measuring slit, minimum analog electronics and an important digital part (computer, interface), are intimately interwoven by an elaborate software. A high degree of automation and sophistication is thus reached. On the one hand, all the photoelectrons coming from each light pulse are integrated. The result, after conversion to digital form enters the computer; a high precision is thus reached: 10(-5) absorbance unit (A.U.) under very favorable conditions, and 10(-4) A.U. for conventional sedimentation studies. On the other hand, during its motion along the image, the precise slit position is permanently known by the system: the sector image is thus segmented into precisely defined zones, inside which the average absorbancies are determined, punched, plotted and printed. New and very useful utilizations and improvements of analytical ultracentrifugation are now possible: the fixed radius mode, permits precise s determination by following the radial dilution; s can be precisely measured by time difference curves even when the solution absorbancy is comparable to the base line variations (0.01 A.U.); round trip analyses permit very long analyses without the absorbancies distributions distorsions due to very long one-way scans during sedimentations runs; reductions of the effect of the stray light by a factor of ten. This system has been in routine use for four years.", "contents": "MaD, an automated precise analytical ultracentrifuge scanner system. The various hardware components of this scanning system, a mechanical split-beam scanner with its photomultiplier measuring slit, minimum analog electronics and an important digital part (computer, interface), are intimately interwoven by an elaborate software. A high degree of automation and sophistication is thus reached. On the one hand, all the photoelectrons coming from each light pulse are integrated. The result, after conversion to digital form enters the computer; a high precision is thus reached: 10(-5) absorbance unit (A.U.) under very favorable conditions, and 10(-4) A.U. for conventional sedimentation studies. On the other hand, during its motion along the image, the precise slit position is permanently known by the system: the sector image is thus segmented into precisely defined zones, inside which the average absorbancies are determined, punched, plotted and printed. New and very useful utilizations and improvements of analytical ultracentrifugation are now possible: the fixed radius mode, permits precise s determination by following the radial dilution; s can be precisely measured by time difference curves even when the solution absorbancy is comparable to the base line variations (0.01 A.U.); round trip analyses permit very long analyses without the absorbancies distributions distorsions due to very long one-way scans during sedimentations runs; reductions of the effect of the stray light by a factor of ten. This system has been in routine use for four years.", "PMID": 963224}
{"id": "pubmed23n0032_5476", "title": "Some general aspects regarding the interpretation of binding data by means of a Scratchard plot.", "content": "The question is quantitatively examined which general information can be obtained from experimental data of ligand binding to macromolecules if represented by a Scatchard plot. In particular, the curvature as well as the intercepts and slopes at both coordinate axes are analyzed assuming rather general conditions including cooperative behavior (with applications to some simplified cases of interest in practice). The results should provide a basis for attempts to elucidate the binding mechanism of a system encountered in experimental work.", "contents": "Some general aspects regarding the interpretation of binding data by means of a Scratchard plot. The question is quantitatively examined which general information can be obtained from experimental data of ligand binding to macromolecules if represented by a Scatchard plot. In particular, the curvature as well as the intercepts and slopes at both coordinate axes are analyzed assuming rather general conditions including cooperative behavior (with applications to some simplified cases of interest in practice). The results should provide a basis for attempts to elucidate the binding mechanism of a system encountered in experimental work.", "PMID": 963225}
{"id": "pubmed23n0032_5477", "title": "Studies on the origin of the tip potential of glass microelectrode.", "content": "1. Tip potential (TP) of glass microelectrodes filled with 3 M KCl increased remarkably with the increase in the storage period in 3 M KCl solution at 37 degrees C, while the electrode resistances decreased gradually. 2. The electrical conductivity through the thin glass wall near the tip was found to increase in parallel with the TP increase. 3. The e.m.f. across the thin glass wall in the tip region was directly measured. This seems to contribute to the TP generation of the microelectrode when the conductivity of the glass wall is significantly high in the tip region. 4. Effects of the acid treatment of glass employed and the acidification of fillant electrolyte solution suggested that fixed negative charges on the glass wall play a fundamental role in the TP formation. 5. Based on these experimental results, it was concluded that not only the diffusion potential through the tip pore but also the interfacial potential through the thin glass wall near the tip contributes to the TP generation, and the contribution of the latter increases with a long exposure period of the electrodes to electrolyte solution. 6. In this connection, technical problems related to reduction of the tip potential were also discussed.", "contents": "Studies on the origin of the tip potential of glass microelectrode. 1. Tip potential (TP) of glass microelectrodes filled with 3 M KCl increased remarkably with the increase in the storage period in 3 M KCl solution at 37 degrees C, while the electrode resistances decreased gradually. 2. The electrical conductivity through the thin glass wall near the tip was found to increase in parallel with the TP increase. 3. The e.m.f. across the thin glass wall in the tip region was directly measured. This seems to contribute to the TP generation of the microelectrode when the conductivity of the glass wall is significantly high in the tip region. 4. Effects of the acid treatment of glass employed and the acidification of fillant electrolyte solution suggested that fixed negative charges on the glass wall play a fundamental role in the TP formation. 5. Based on these experimental results, it was concluded that not only the diffusion potential through the tip pore but also the interfacial potential through the thin glass wall near the tip contributes to the TP generation, and the contribution of the latter increases with a long exposure period of the electrodes to electrolyte solution. 6. In this connection, technical problems related to reduction of the tip potential were also discussed.", "PMID": 963226}
{"id": "pubmed23n0032_5478", "title": "Subunit symmetry of tetrameric phosphorylase a.", "content": "Native crystallographic data of tetrameric phosphorylase a crystals, space group P21, have been collected photographically to 3 A resolution. These data have been used in Patterson search methods in reciprocal and real space. The tetramers were found to exhibit molecular 222 symmetry. The cross vector between the centres of the two symmetry related tetramers in the unit cell was determined by two different translation function methods. On the basis of these rotation and translation function results a model for the arrangement of monomers within the tetramer and of tetramers in the unit cell is proposed; The 222 symmetry of the tetrameric molecule is found only when high resolution diffraction data are included (i.e. higher than 6 A). At lower resolution other symmetries dominate. Calculations with the proposed model have shown that these spurious symmetries result from the nonspecific overlap of protein-protein and solvent-solvent cross vectors. These results emphasize the importance of high resolution data when noncrystallographic symmetry of globular proteins is studied.", "contents": "Subunit symmetry of tetrameric phosphorylase a. Native crystallographic data of tetrameric phosphorylase a crystals, space group P21, have been collected photographically to 3 A resolution. These data have been used in Patterson search methods in reciprocal and real space. The tetramers were found to exhibit molecular 222 symmetry. The cross vector between the centres of the two symmetry related tetramers in the unit cell was determined by two different translation function methods. On the basis of these rotation and translation function results a model for the arrangement of monomers within the tetramer and of tetramers in the unit cell is proposed; The 222 symmetry of the tetrameric molecule is found only when high resolution diffraction data are included (i.e. higher than 6 A). At lower resolution other symmetries dominate. Calculations with the proposed model have shown that these spurious symmetries result from the nonspecific overlap of protein-protein and solvent-solvent cross vectors. These results emphasize the importance of high resolution data when noncrystallographic symmetry of globular proteins is studied.", "PMID": 963227}
{"id": "pubmed23n0032_5479", "title": "Measurements on fast light-induced light-scattering and -absorption changes in outer segments of vertebrate light sensitive rod cells.", "content": "Flash-induced changes of light-absorption and of light-scattering of vertebrate rod outer segments (ROS) from frog and cattle in suspension were measured at 380 and 800 nm. The photometer used allows the observation of light intensity changes under well defined angles. We studied the successive decrease of the signal amplitude in series of flashes. One flash bleaches about 1% rhodopsin. The following results are discussed: 1. The signal at 380 nm is a superposition of the absorption change caused by formation of metarhodopsin II and of a biphasic additional signal. The latter exists only for the initial range of bleaching (15 to 25% rhodopsin). 2. At 800 nm three scattering signals are observed which are characterized by their successive amplitude decrease and time course: N: A small signal with time course and successive amplitude decrease comparable to the metarhodopsin II absorption change, probably arising from a structural change within the disc membrane. Ni: A slow signal, disappearing with the first flash, which may be understood as an outer membrane effect. P: A biphasic signal with a successive decrease rate, by a factor of 10 to 20 higher than that of the metarhodopsin II signal. The two kinetically different components are separated by variation of the observation angle. Two regions of different extension appear to change structurally with different time course. \"P\" may reflect an influence of the light-induced transmitter release on disc shape and/or mass.", "contents": "Measurements on fast light-induced light-scattering and -absorption changes in outer segments of vertebrate light sensitive rod cells. Flash-induced changes of light-absorption and of light-scattering of vertebrate rod outer segments (ROS) from frog and cattle in suspension were measured at 380 and 800 nm. The photometer used allows the observation of light intensity changes under well defined angles. We studied the successive decrease of the signal amplitude in series of flashes. One flash bleaches about 1% rhodopsin. The following results are discussed: 1. The signal at 380 nm is a superposition of the absorption change caused by formation of metarhodopsin II and of a biphasic additional signal. The latter exists only for the initial range of bleaching (15 to 25% rhodopsin). 2. At 800 nm three scattering signals are observed which are characterized by their successive amplitude decrease and time course: N: A small signal with time course and successive amplitude decrease comparable to the metarhodopsin II absorption change, probably arising from a structural change within the disc membrane. Ni: A slow signal, disappearing with the first flash, which may be understood as an outer membrane effect. P: A biphasic signal with a successive decrease rate, by a factor of 10 to 20 higher than that of the metarhodopsin II signal. The two kinetically different components are separated by variation of the observation angle. Two regions of different extension appear to change structurally with different time course. \"P\" may reflect an influence of the light-induced transmitter release on disc shape and/or mass.", "PMID": 963228}
{"id": "pubmed23n0032_5480", "title": "Evidence for chromophore-chromophore interactions in the purple membrane from reconstitution experiments of the chromophore-free membrane.", "content": "We recently presented evidence showing that the visible CD spectrum of the purple membrane from Halobacterium halobium consists of two contributions: a broad positive band centered at the absorption maximum due to the interaction of the chromophore with the protein to which it is bound, and an exciton coupling band due to the interaction between chromophores of adjacent bacteriohodopsin molecules in the hexagonal surface lattice (Heyn et al., 1975); This interpretation receives strong support from the present experiments in which the chromophore-free membrane is reconstituted by the addition of retinal. Since the coupling signal arises from the interaction between pairs of neighboring chromophores, its contribution to the spectrum would be expected to be very small in the initial stages of the titration experiment, but increasing quadratically with the percentage reconstitution. The broad positive band, on the other hand, is expected to increase linearly with the percentage reconstitution. On the basis of these considerations a satisfactory explanation of the CD reconstitution experiments could be given. Since it appears to be impossible to explain the titration experiments without the quadratic term, we conclude that chromophore-chromophore interactions play an important role. No significant changes in secondary structure upon reconstitution should be detected consistent with our binding model which neglects cooperativity;", "contents": "Evidence for chromophore-chromophore interactions in the purple membrane from reconstitution experiments of the chromophore-free membrane. We recently presented evidence showing that the visible CD spectrum of the purple membrane from Halobacterium halobium consists of two contributions: a broad positive band centered at the absorption maximum due to the interaction of the chromophore with the protein to which it is bound, and an exciton coupling band due to the interaction between chromophores of adjacent bacteriohodopsin molecules in the hexagonal surface lattice (Heyn et al., 1975); This interpretation receives strong support from the present experiments in which the chromophore-free membrane is reconstituted by the addition of retinal. Since the coupling signal arises from the interaction between pairs of neighboring chromophores, its contribution to the spectrum would be expected to be very small in the initial stages of the titration experiment, but increasing quadratically with the percentage reconstitution. The broad positive band, on the other hand, is expected to increase linearly with the percentage reconstitution. On the basis of these considerations a satisfactory explanation of the CD reconstitution experiments could be given. Since it appears to be impossible to explain the titration experiments without the quadratic term, we conclude that chromophore-chromophore interactions play an important role. No significant changes in secondary structure upon reconstitution should be detected consistent with our binding model which neglects cooperativity;", "PMID": 963229}
{"id": "pubmed23n0032_5481", "title": "Some similarities between processes at biological membranes and lipid bilayers.", "content": "Several processes at biological membranes can be simulated by experiments with artificial lipid bilayer membranes. Three selected examples are discussed: The uncoupler induced proton permeability of lipid bilayers, the initiation of action potential like voltage responses in lipid membranes, and the reconstitution of active cation pumps across planar lipid bilayers or lipid vesicles.", "contents": "Some similarities between processes at biological membranes and lipid bilayers. Several processes at biological membranes can be simulated by experiments with artificial lipid bilayer membranes. Three selected examples are discussed: The uncoupler induced proton permeability of lipid bilayers, the initiation of action potential like voltage responses in lipid membranes, and the reconstitution of active cation pumps across planar lipid bilayers or lipid vesicles.", "PMID": 963230}
{"id": "pubmed23n0032_5482", "title": "Investigation of the relation between structure and function in myelinated nerve fibres with be aid of ultraviolet radiation.", "content": "Ultraviolet radiation induces two photochemical alterations relevant to excitability in the nodal membranes: A selective blocking of the sodium permeability and a potential translation of the voltage dependent kinetic parameters of sodium inactivation and activation along the potential axis in the negative direction. The underlying processes are two different photoreactions, since 1) the action spectrum of the blocking effect shows a marked peak near 280 nm and rapidly decreasing sensitivity towards higher and lower wavelengths, while the action spectrum of the potential shift increase with lower wavelengths; 2) the blocking effect is enhanced by a more positive holding potential, while the potential shift is decreased; 3) the potential shift can be prevented intraaxonal application of l-cysteine or 2-mercaptoethanol, but the blocking effect is not affected.", "contents": "Investigation of the relation between structure and function in myelinated nerve fibres with be aid of ultraviolet radiation. Ultraviolet radiation induces two photochemical alterations relevant to excitability in the nodal membranes: A selective blocking of the sodium permeability and a potential translation of the voltage dependent kinetic parameters of sodium inactivation and activation along the potential axis in the negative direction. The underlying processes are two different photoreactions, since 1) the action spectrum of the blocking effect shows a marked peak near 280 nm and rapidly decreasing sensitivity towards higher and lower wavelengths, while the action spectrum of the potential shift increase with lower wavelengths; 2) the blocking effect is enhanced by a more positive holding potential, while the potential shift is decreased; 3) the potential shift can be prevented intraaxonal application of l-cysteine or 2-mercaptoethanol, but the blocking effect is not affected.", "PMID": 963231}
{"id": "pubmed23n0032_5483", "title": "The structure of two alanine containing ferrichromes: sequence determination by proton magnetic resonance.", "content": "Metal coordination confers an extraordinary structural stability to the ferrichromes which, independent of their variable amino acid composition, results in a basically unperturbed conformation for all the homologous peptides in the series. The proton magnetic resonance (pmr) characteristics for Al3+ analogues (alumichromes) reflect this conformational isomorphism in usual solvents so that single site substitutions are clearly recognized in the pmr spectra. Thus, the substitution of glycine by L-alanine or L-serine introduce new resonances characteristic of the sidechains and alter the pattern of the amide NH pmr region in that doublets substitute for glycyl triplets at the same site. Since for glycine- and L-serine containing alumichromes the resonances have already been identified, it is possible to unequivocally establish the primary structure of the two L-alanyl homologues ferrichrome C (see article) and sake colorant A (see article) on the basis of the comparative pmr spectra of their Al3+ analogues, namely, alumichrome C and alumisake. The resonance assignment, and hence the site occupancy, is substantiated by the temperature coefficients of the NH chemical shifts, rates of 1H-2H exchange and homonuclear proton spin decoupling experiments centered on the NH spectral region. Occupancy of site 1 by a glycine residue is observed for all known ferrichromes, which serves to conserve a \"hairpin\" turn. This method of obtaining sequence information should prove of general use for other systems of homologous polypeptides, provided their conformations are not affected by the residue substitutions.", "contents": "The structure of two alanine containing ferrichromes: sequence determination by proton magnetic resonance. Metal coordination confers an extraordinary structural stability to the ferrichromes which, independent of their variable amino acid composition, results in a basically unperturbed conformation for all the homologous peptides in the series. The proton magnetic resonance (pmr) characteristics for Al3+ analogues (alumichromes) reflect this conformational isomorphism in usual solvents so that single site substitutions are clearly recognized in the pmr spectra. Thus, the substitution of glycine by L-alanine or L-serine introduce new resonances characteristic of the sidechains and alter the pattern of the amide NH pmr region in that doublets substitute for glycyl triplets at the same site. Since for glycine- and L-serine containing alumichromes the resonances have already been identified, it is possible to unequivocally establish the primary structure of the two L-alanyl homologues ferrichrome C (see article) and sake colorant A (see article) on the basis of the comparative pmr spectra of their Al3+ analogues, namely, alumichrome C and alumisake. The resonance assignment, and hence the site occupancy, is substantiated by the temperature coefficients of the NH chemical shifts, rates of 1H-2H exchange and homonuclear proton spin decoupling experiments centered on the NH spectral region. Occupancy of site 1 by a glycine residue is observed for all known ferrichromes, which serves to conserve a \"hairpin\" turn. This method of obtaining sequence information should prove of general use for other systems of homologous polypeptides, provided their conformations are not affected by the residue substitutions.", "PMID": 963232}
{"id": "pubmed23n0032_5484", "title": "Optical and electrical studies on dansyllysine-valinomycin in thin lipid membranes.", "content": "Dansyllysine-valinomycin, a fluorescent analogue of the ionophore valinomycin was synthesized and incorporated into black lipid membranes. Its concentration inside the membrane was measured fluorometrically and was also determined from electrical relaxation experiments, which were analyzed on the basis of a previously proposed carrier model. The results of both methods agreed within less than one order of magnitude. This appears satisfactory in view of the sources of error inherent in both procedures. A conductance increment per carrier molecule of about 3 - 10(-17) omega-1 was obtained for dansyllysine-valinomycin in diphytanoyllecithin membranes at 25 degrees C and 1 M RbCl in the aqueous phases. This is about 400 times smaller compared to unmodified valinomycin in monoolein membranes. The difference is mainly caused by the change in the membrane properties and to a smaller extent by the structural modification of the carrier.", "contents": "Optical and electrical studies on dansyllysine-valinomycin in thin lipid membranes. Dansyllysine-valinomycin, a fluorescent analogue of the ionophore valinomycin was synthesized and incorporated into black lipid membranes. Its concentration inside the membrane was measured fluorometrically and was also determined from electrical relaxation experiments, which were analyzed on the basis of a previously proposed carrier model. The results of both methods agreed within less than one order of magnitude. This appears satisfactory in view of the sources of error inherent in both procedures. A conductance increment per carrier molecule of about 3 - 10(-17) omega-1 was obtained for dansyllysine-valinomycin in diphytanoyllecithin membranes at 25 degrees C and 1 M RbCl in the aqueous phases. This is about 400 times smaller compared to unmodified valinomycin in monoolein membranes. The difference is mainly caused by the change in the membrane properties and to a smaller extent by the structural modification of the carrier.", "PMID": 963233}
{"id": "pubmed23n0032_5485", "title": "The effect of temperature on canine papillary muscle.", "content": "Measurements of the total duration, tA, of the action potential for canine papillary muscle in the temperature range 25-45 degrees C are shown to follow the law ta = tau exp (Q/kBT), with tau and the activation energy Q independent of temperature, T, and kB equal to Boltzmann's constant. The value obtained for Q = 0.65 +/- 0.10 eV. Results are also presented for the temperature dependence of points V(x) on the action potential curve, where x is the percentage of repolarisation within the range 30% less than x less than 100%.", "contents": "The effect of temperature on canine papillary muscle. Measurements of the total duration, tA, of the action potential for canine papillary muscle in the temperature range 25-45 degrees C are shown to follow the law ta = tau exp (Q/kBT), with tau and the activation energy Q independent of temperature, T, and kB equal to Boltzmann's constant. The value obtained for Q = 0.65 +/- 0.10 eV. Results are also presented for the temperature dependence of points V(x) on the action potential curve, where x is the percentage of repolarisation within the range 30% less than x less than 100%.", "PMID": 963234}
{"id": "pubmed23n0032_5486", "title": "Applications of computerized pattern recognition: a survey of correlations between pharmacological activities and mass spectra.", "content": "Correlations between mass spectra and pharmacological activities were surveyed by means of computerized learning machine and cluster analysis. The principal component analysis and the nonlinear mapping techniques were used for the feature selection of the data set.", "contents": "Applications of computerized pattern recognition: a survey of correlations between pharmacological activities and mass spectra. Correlations between mass spectra and pharmacological activities were surveyed by means of computerized learning machine and cluster analysis. The principal component analysis and the nonlinear mapping techniques were used for the feature selection of the data set.", "PMID": 963271}
{"id": "pubmed23n0032_5487", "title": "Mass spectral study of derivatives of the four bilirubin-IX isomers.", "content": "The beta, gamma and delta isomers of bilirubin-IX show a decreased stability as compared with the IXalpha isomer; characteristic mass spectra are obtained only for the tetrakis-(trimethylsilyl) derivatives of the IXalpha and IXgamma isomers. Hydrogenation of the vinyl substituents increases the thermal stability of the bilirubins and gives rise to a characteristic mass spectrum for the tetrakis-(trimethylsilyl) derivative of meso-bilirubin-IXdelta. The ethyl anthranilate azopigments derived from the four bilirubins yield characteristic mass spectra, except for the two unstable divinyl substituted azodipyrroles (mol. wt. 416), derived from bilirubin-IXbeta and IXdelta. The corresponding mol. wt. 420 azopigments derived from the hydrogenated bilirubins are thermally stable. Elucidation of mass spectral fragmentation pathways is facilitated by the varying positions of the substituents and by deuterium labelling, and permits the assignment of structures to the various isomeric azodipyrroles.", "contents": "Mass spectral study of derivatives of the four bilirubin-IX isomers. The beta, gamma and delta isomers of bilirubin-IX show a decreased stability as compared with the IXalpha isomer; characteristic mass spectra are obtained only for the tetrakis-(trimethylsilyl) derivatives of the IXalpha and IXgamma isomers. Hydrogenation of the vinyl substituents increases the thermal stability of the bilirubins and gives rise to a characteristic mass spectrum for the tetrakis-(trimethylsilyl) derivative of meso-bilirubin-IXdelta. The ethyl anthranilate azopigments derived from the four bilirubins yield characteristic mass spectra, except for the two unstable divinyl substituted azodipyrroles (mol. wt. 416), derived from bilirubin-IXbeta and IXdelta. The corresponding mol. wt. 420 azopigments derived from the hydrogenated bilirubins are thermally stable. Elucidation of mass spectral fragmentation pathways is facilitated by the varying positions of the substituents and by deuterium labelling, and permits the assignment of structures to the various isomeric azodipyrroles.", "PMID": 963272}
{"id": "pubmed23n0032_5488", "title": "Trimethylsilyl group migration during electron impact and chemical ionization mass spectrometry of the trimethylsilyl ethers of 20-hydroxy-5alpha-pregnan-3-ones and 20-hydroxy-4-pregnen-3-ones.", "content": "The electron impact and chemical ionization (isobutane) mass spectra of the TMS derivatives of 20-hydroxy-5alpha-pregnan-3-ones and 20-hydroxy-4-pregnen-3-ones include ions [M--44]+-attributable to loss of a CH3CHO fragment from C-17 with migration of the TMS group to the charge-retaining fragment. Mass spectra of isotopically labelled ([3-18O], [20-18O] and [2H9-TMS]) analogues are consistent with this mechanism; [2H9-TMS] labelling further indicates that subsequent loss of a methyl group from [M--44]+-ions does not involve the TMS group. Corresponding ions are not observed at significant abundance in the spectra of 20beta-trimethylsilyloxy-5alpha-pregnane and 20beta-trimethylsilyloxy-4-pregnen-3-one 3-O-methyloxime. In the electron impact mass spectrum of the t-butyldimethylsilyl ether of 20beta-hydroxy-4-pregnen-3-one, an ion is observed corresponding to loss of 44 atomic mass units from the intense [M--C4H9]+ ion.", "contents": "Trimethylsilyl group migration during electron impact and chemical ionization mass spectrometry of the trimethylsilyl ethers of 20-hydroxy-5alpha-pregnan-3-ones and 20-hydroxy-4-pregnen-3-ones. The electron impact and chemical ionization (isobutane) mass spectra of the TMS derivatives of 20-hydroxy-5alpha-pregnan-3-ones and 20-hydroxy-4-pregnen-3-ones include ions [M--44]+-attributable to loss of a CH3CHO fragment from C-17 with migration of the TMS group to the charge-retaining fragment. Mass spectra of isotopically labelled ([3-18O], [20-18O] and [2H9-TMS]) analogues are consistent with this mechanism; [2H9-TMS] labelling further indicates that subsequent loss of a methyl group from [M--44]+-ions does not involve the TMS group. Corresponding ions are not observed at significant abundance in the spectra of 20beta-trimethylsilyloxy-5alpha-pregnane and 20beta-trimethylsilyloxy-4-pregnen-3-one 3-O-methyloxime. In the electron impact mass spectrum of the t-butyldimethylsilyl ether of 20beta-hydroxy-4-pregnen-3-one, an ion is observed corresponding to loss of 44 atomic mass units from the intense [M--C4H9]+ ion.", "PMID": 963273}
{"id": "pubmed23n0032_5489", "title": "Mass spectrometry of N-acylated daunorubicin derivatives.", "content": "The mass spectra of peracetylated daunorubicin, N-octanoyl-, N-dodecanoyl- and N-(N'-dodecanoyl-glycyl)daunorubicin, and perdeuteroacetylated N-dodecanoyldaunorubicin were analyzed. Major fragmentation pathways were suggested and ion compositions were determined by high resolution measurements.", "contents": "Mass spectrometry of N-acylated daunorubicin derivatives. The mass spectra of peracetylated daunorubicin, N-octanoyl-, N-dodecanoyl- and N-(N'-dodecanoyl-glycyl)daunorubicin, and perdeuteroacetylated N-dodecanoyldaunorubicin were analyzed. Major fragmentation pathways were suggested and ion compositions were determined by high resolution measurements.", "PMID": 963274}
{"id": "pubmed23n0032_5490", "title": "Field desorption mass spectrometry of phospholipids. II--Fragmentation of dipalmitoylphosphatidyl choline from comparison of d0, d4 and d9 species.", "content": "Field desorption mass spectra have been obtained for dipalmitoylphosphatidyl choline-d0, -d4 and -d9. The two latter compounds were synthesized from dimethylene-d4 choline bromide and trimethyl-d9 choline bromide, respectively. Comparison was made of spectra of these three compounds obtained at different field anode temperatures in order to establish the composition of the major cleavage, rearrangement, and association ions. At low anode heating currents (less than or equal to 20 mA) the most intense peak is [M + 1]+ which accounts for c. one-third of the total ionization. At higher temperatures various fragmentation processes, some of which are without analogy in electron impact mass spectrometry, become more important. The presence of an intermolecular methyl transfer process had been confirmed.", "contents": "Field desorption mass spectrometry of phospholipids. II--Fragmentation of dipalmitoylphosphatidyl choline from comparison of d0, d4 and d9 species. Field desorption mass spectra have been obtained for dipalmitoylphosphatidyl choline-d0, -d4 and -d9. The two latter compounds were synthesized from dimethylene-d4 choline bromide and trimethyl-d9 choline bromide, respectively. Comparison was made of spectra of these three compounds obtained at different field anode temperatures in order to establish the composition of the major cleavage, rearrangement, and association ions. At low anode heating currents (less than or equal to 20 mA) the most intense peak is [M + 1]+ which accounts for c. one-third of the total ionization. At higher temperatures various fragmentation processes, some of which are without analogy in electron impact mass spectrometry, become more important. The presence of an intermolecular methyl transfer process had been confirmed.", "PMID": 963275}
{"id": "pubmed23n0032_5491", "title": "Electron impact-induced reactions of N6-(3-methyl-2-butenyl)adenosine and related cytokinins.", "content": "Fragmentation reactions of the biologically important N6-(3-methyl-2-butenyl)adenyl moiety have been re-examined with the aid of systematic deuterium labeling in the sidechain and by examination of the 1- and 7-deazanucleoside analogs. It is concluded that the diagnostic reactions which involve expulsion of C3H7 proceed predominantly by ring closure from the sidechain double bond to N-1 (ion a). Base-containing ions m/e 135 and 148 were confirmed to arise mainly by rearrangement of hydrogen from the methyl terminus to N6 and simple cleavage, respectively, but with significant contribution from other pathways involving transfer of sidechain hydrogens to the base.", "contents": "Electron impact-induced reactions of N6-(3-methyl-2-butenyl)adenosine and related cytokinins. Fragmentation reactions of the biologically important N6-(3-methyl-2-butenyl)adenyl moiety have been re-examined with the aid of systematic deuterium labeling in the sidechain and by examination of the 1- and 7-deazanucleoside analogs. It is concluded that the diagnostic reactions which involve expulsion of C3H7 proceed predominantly by ring closure from the sidechain double bond to N-1 (ion a). Base-containing ions m/e 135 and 148 were confirmed to arise mainly by rearrangement of hydrogen from the methyl terminus to N6 and simple cleavage, respectively, but with significant contribution from other pathways involving transfer of sidechain hydrogens to the base.", "PMID": 963276}
{"id": "pubmed23n0032_5492", "title": "Quantitative determination of salbutamol in plasma, as either its trimethylsilyl or t-butyldimethylsilyl ether, using a stable isotope multiple ion recording technique.", "content": "Two methods are described for the determination of salbutamol in human plasma. The drug is extracted from the plasma as a salbutamol tetraphenylboron ion pair and determined by gas chromatography mass spectrometry. Trideuterio-salbutamol is used as an internal standard. In the first method an extensive purification procedure is used to separate salbutamol from plasma cholesterol which interferes in the assay. Salbutamol is then determined as its TMS-ether using a multiple ion recording technique to measure the intensity of the fragment ion m/e 369 and the ion m/e 372 from the TMS ether of trideuterio-salbutamol. The second method is based on the ion pair extraction of salbutamol into heptan-3-one and its determination by gas chromatography mass spectrometry as the t-butyldimethylsilyl ether. The base peak in the mass spectrum of the t-butyldimethylsilyl-salbutamol is an ion of m/e 495, which is of sufficiently high mass to distinguish it from any of the ions which arise from t butyldimethylsilyl-cholesterol. Six replicate analyses of plasma samples containing 1 ng salbutamol ml-1 were carried out using both methods. When the first method was used the mean value obtained was 1.3 ng ml-1 and the coefficient of variation was 17.7%. When the second method was used the mean value obtained was 0.95 ng ml-1 and the coefficient of variation was 10%. The second method is more rapid and therefore preferable for use in clinical pharmacological studies. This method has been used to determine the plasma salbutamol concentrations at varying times after either a 4 mg oral or a 200 mug intravenous dose of salbutamol to man.", "contents": "Quantitative determination of salbutamol in plasma, as either its trimethylsilyl or t-butyldimethylsilyl ether, using a stable isotope multiple ion recording technique. Two methods are described for the determination of salbutamol in human plasma. The drug is extracted from the plasma as a salbutamol tetraphenylboron ion pair and determined by gas chromatography mass spectrometry. Trideuterio-salbutamol is used as an internal standard. In the first method an extensive purification procedure is used to separate salbutamol from plasma cholesterol which interferes in the assay. Salbutamol is then determined as its TMS-ether using a multiple ion recording technique to measure the intensity of the fragment ion m/e 369 and the ion m/e 372 from the TMS ether of trideuterio-salbutamol. The second method is based on the ion pair extraction of salbutamol into heptan-3-one and its determination by gas chromatography mass spectrometry as the t-butyldimethylsilyl ether. The base peak in the mass spectrum of the t-butyldimethylsilyl-salbutamol is an ion of m/e 495, which is of sufficiently high mass to distinguish it from any of the ions which arise from t butyldimethylsilyl-cholesterol. Six replicate analyses of plasma samples containing 1 ng salbutamol ml-1 were carried out using both methods. When the first method was used the mean value obtained was 1.3 ng ml-1 and the coefficient of variation was 17.7%. When the second method was used the mean value obtained was 0.95 ng ml-1 and the coefficient of variation was 10%. The second method is more rapid and therefore preferable for use in clinical pharmacological studies. This method has been used to determine the plasma salbutamol concentrations at varying times after either a 4 mg oral or a 200 mug intravenous dose of salbutamol to man.", "PMID": 963277}
{"id": "pubmed23n0032_5493", "title": "The use of N-succinyl derivatives in the study of amino acids and peptides by mass spectrometry.", "content": "The terminal amino group of amino acids and peptides is blocked as the N-succinyl derivative by reaction with succinic anhydride. The product is then converted to the N,O-permethyl derivative in order to increase its volatility for use in mass spectrometry. The permethylated N-succinyl derivative retains the advantages of the permethylated N-acetyl derivative in regard to ease of preparation on a small scale, volatility and the presence of characteristic fragmentation patterns in their mass spectra. However, peaks in the high mass region are more abundant due to loss of CH3O-from the N-succinyl carbomethoxyl group as well as from the C-terminal carbomethoxyl group. Ions characteristic of the sequence and of individual amino acids are observed, and molecular weight can be determined from the relatively abundant ion at [M--CH3O]+ and from the weak molecular ion.", "contents": "The use of N-succinyl derivatives in the study of amino acids and peptides by mass spectrometry. The terminal amino group of amino acids and peptides is blocked as the N-succinyl derivative by reaction with succinic anhydride. The product is then converted to the N,O-permethyl derivative in order to increase its volatility for use in mass spectrometry. The permethylated N-succinyl derivative retains the advantages of the permethylated N-acetyl derivative in regard to ease of preparation on a small scale, volatility and the presence of characteristic fragmentation patterns in their mass spectra. However, peaks in the high mass region are more abundant due to loss of CH3O-from the N-succinyl carbomethoxyl group as well as from the C-terminal carbomethoxyl group. Ions characteristic of the sequence and of individual amino acids are observed, and molecular weight can be determined from the relatively abundant ion at [M--CH3O]+ and from the weak molecular ion.", "PMID": 963278}
{"id": "pubmed23n0032_5494", "title": "Identification of N-nitrosodimethylamine in ambient air by capillary gas-liquid chromatography mass spectrometry computer.", "content": "Organic vapors in ambient air, at or near an industrial site in Baltimore, Maryland, were collected by adsorption on a sorbent of porous polymer (Tenax GC, 35/60). The pollutants were recovered by thermal desorption and analyzed by gas-liquid chromatography mass spectrometry computer using capillary glass SCOT columns. N-Nitrosodimethylamine was identified from its mass spectrum as a constituent of the atmosphere in the areas sampled. The identification of this compound was confirmed by comparison of its mass spectrum with that of authentic N-nitrosodimethylamine; identical retention times of unknown and authentic compound on three different capillary columns were observed.", "contents": "Identification of N-nitrosodimethylamine in ambient air by capillary gas-liquid chromatography mass spectrometry computer. Organic vapors in ambient air, at or near an industrial site in Baltimore, Maryland, were collected by adsorption on a sorbent of porous polymer (Tenax GC, 35/60). The pollutants were recovered by thermal desorption and analyzed by gas-liquid chromatography mass spectrometry computer using capillary glass SCOT columns. N-Nitrosodimethylamine was identified from its mass spectrum as a constituent of the atmosphere in the areas sampled. The identification of this compound was confirmed by comparison of its mass spectrum with that of authentic N-nitrosodimethylamine; identical retention times of unknown and authentic compound on three different capillary columns were observed.", "PMID": 963279}
{"id": "pubmed23n0032_5495", "title": "Use of a tapered fluidized bed as a continuous bioreactor.", "content": "Reactor systems based on tapered fluidized beds are being developed for aqueous bioprocesses in which adhering microorganisms or immobilized active biological fractions are used. The use of a fluidized bed prevents biomass buildup, accommodates particulates in the feed stream, is compatible with gas sparging, and allows easy removal or addition of the active materials. The tapered reactor tends to stabilize the fluidized bed, thus allowing a much wider range of operating conditions. Preliminary experimental results and an empirical mathematical model of the tapered bed indicate that bed stability is associated with a decreasing velocity and void-fraction profile up the bed and the pressure drop across the bed decreases with increasing flow rates. The tapered fluidized bed bioreactor is being evaluated for use in the enzymatic production of hydrogen, microbiological denitrification, and microbiological degradation of coal conversion aqueous waste streams. The enzyme catalyzed conversion of lactose to glucose and galactose was used in the evaluation of the reactor concept.", "contents": "Use of a tapered fluidized bed as a continuous bioreactor. Reactor systems based on tapered fluidized beds are being developed for aqueous bioprocesses in which adhering microorganisms or immobilized active biological fractions are used. The use of a fluidized bed prevents biomass buildup, accommodates particulates in the feed stream, is compatible with gas sparging, and allows easy removal or addition of the active materials. The tapered reactor tends to stabilize the fluidized bed, thus allowing a much wider range of operating conditions. Preliminary experimental results and an empirical mathematical model of the tapered bed indicate that bed stability is associated with a decreasing velocity and void-fraction profile up the bed and the pressure drop across the bed decreases with increasing flow rates. The tapered fluidized bed bioreactor is being evaluated for use in the enzymatic production of hydrogen, microbiological denitrification, and microbiological degradation of coal conversion aqueous waste streams. The enzyme catalyzed conversion of lactose to glucose and galactose was used in the evaluation of the reactor concept.", "PMID": 963280}
{"id": "pubmed23n0032_5496", "title": "Automatic membrane-filtration system for the \"on-demand\" supply of large volumes of sterile medium in continuous culture.", "content": "The construction and use of an automated membrane filtration system is described which produces 300 liters per day of sterile medium over at least four months of continuous fermentation. The capacity of the system is limited only by choice of filter type and has applications in continuous pilot plant work or medium-scale batch fermentation, in the bulk supply of communally used laboratory medium, in supplying banks of small-scale continuous fermentors, and for remote control of medium supply in hazardous fermentations (e.g., methane) with great savings in time and materials. Only two sterile storage jars are needed, irrespective of the total demand for the medium.", "contents": "Automatic membrane-filtration system for the \"on-demand\" supply of large volumes of sterile medium in continuous culture. The construction and use of an automated membrane filtration system is described which produces 300 liters per day of sterile medium over at least four months of continuous fermentation. The capacity of the system is limited only by choice of filter type and has applications in continuous pilot plant work or medium-scale batch fermentation, in the bulk supply of communally used laboratory medium, in supplying banks of small-scale continuous fermentors, and for remote control of medium supply in hazardous fermentations (e.g., methane) with great savings in time and materials. Only two sterile storage jars are needed, irrespective of the total demand for the medium.", "PMID": 963281}
{"id": "pubmed23n0032_5497", "title": "Aerobic microbial growth in semisolid matrices: heat and mass transfer limitation.", "content": "A conceptual model of aerobic microbial growth in semisolid matrices were developed as a first step in the prediction of the rate of breakdown in semisolid cellulosic material. The conceptual model was described by a series of equations simplified by the assumption of steady-state microbial activity, and heat and mass transfer limitation. Temperature and oxygen distribution in compost piles were measured experimentally at the Butler County Mushroom Farm, Butler County, Pennsylvania, to test the validity of these assumptions. The compost piles consisted of ground corn husks, straw, and race horse manure. The data fit with the model was excellent with deviation between model predictions (as solved by an anglog computer) and actual temperature measurements never exceeding 3 degrees C. The effects of compost pile geometry, external temperature, compost density, external oxygen concentration, and insulation at the bottom of the pile were then predicted using a digital computer to solve the model. The predicitions show that the maximum breakdown rate occurs for an optimum height (which depends upon the system), insulating the base increases the breakdown rate, increasing the external temperature increases the initial breakdown rate but decreases the pseudo-steady-state breakdown rate and the uniformity and any increase in the external oxygen concentration increases the breakdown rate but decreases the uniformity.", "contents": "Aerobic microbial growth in semisolid matrices: heat and mass transfer limitation. A conceptual model of aerobic microbial growth in semisolid matrices were developed as a first step in the prediction of the rate of breakdown in semisolid cellulosic material. The conceptual model was described by a series of equations simplified by the assumption of steady-state microbial activity, and heat and mass transfer limitation. Temperature and oxygen distribution in compost piles were measured experimentally at the Butler County Mushroom Farm, Butler County, Pennsylvania, to test the validity of these assumptions. The compost piles consisted of ground corn husks, straw, and race horse manure. The data fit with the model was excellent with deviation between model predictions (as solved by an anglog computer) and actual temperature measurements never exceeding 3 degrees C. The effects of compost pile geometry, external temperature, compost density, external oxygen concentration, and insulation at the bottom of the pile were then predicted using a digital computer to solve the model. The predicitions show that the maximum breakdown rate occurs for an optimum height (which depends upon the system), insulating the base increases the breakdown rate, increasing the external temperature increases the initial breakdown rate but decreases the pseudo-steady-state breakdown rate and the uniformity and any increase in the external oxygen concentration increases the breakdown rate but decreases the uniformity.", "PMID": 963286}
{"id": "pubmed23n0032_5498", "title": "Utilization of cellulosic materials through enzymatic hydrolysis. II. Preliminary assessment of an integrated processing scheme.", "content": "An integrated processing scheme is described for the conversion of a celluosic waste (newsprint) to sugrsa by enzymatic hydrolysis and then to ethanol and yeast by fermentation. The unconverted solids are burned to produce process energy requirements and surplus electrical power. Preliminary designs and cost studies are developed to provide a rough perspective on the potential economic feasibility of this method of cellulose utilization.", "contents": "Utilization of cellulosic materials through enzymatic hydrolysis. II. Preliminary assessment of an integrated processing scheme. An integrated processing scheme is described for the conversion of a celluosic waste (newsprint) to sugrsa by enzymatic hydrolysis and then to ethanol and yeast by fermentation. The unconverted solids are burned to produce process energy requirements and surplus electrical power. Preliminary designs and cost studies are developed to provide a rough perspective on the potential economic feasibility of this method of cellulose utilization.", "PMID": 963287}
{"id": "pubmed23n0032_5499", "title": "A clinical study of the lupus anticoagulant.", "content": "Eighty-three patients with circulating anticoagulants were studied at The New York Hospital. The lupus-type anticoagulant, an inhibitor of the prothrombin activator complex, was demonstrated in 58 patients. The inhibitor was identified using the blood and tissue thromboplastin inhibition tests. Inhibition by the lupus anticoagulant was augmented in 67% of these patients by a cofactor present in normal plasma. The lupus inhibitor was detected primarily because of an unsuspected abnormal coagulation test. One-half of the patients with the lupus-type anticoagulant did not have systemic lupus erythematosus.", "contents": "A clinical study of the lupus anticoagulant. Eighty-three patients with circulating anticoagulants were studied at The New York Hospital. The lupus-type anticoagulant, an inhibitor of the prothrombin activator complex, was demonstrated in 58 patients. The inhibitor was identified using the blood and tissue thromboplastin inhibition tests. Inhibition by the lupus anticoagulant was augmented in 67% of these patients by a cofactor present in normal plasma. The lupus inhibitor was detected primarily because of an unsuspected abnormal coagulation test. One-half of the patients with the lupus-type anticoagulant did not have systemic lupus erythematosus.", "PMID": 963290}
{"id": "pubmed23n0032_5500", "title": "Acquired storage pool disease in platelets during disseminated intravascular coagulation.", "content": "A patient with clinical and laboratory evidence of disseminated intravascular coagulation associated with deep-vein thrombosis and pulmonary embolism developed a qualitative platelet abnormality characterized by a defective release reaction. Second-phase aggregation induced by ADP and adrenaline was impaired, and reduced collagen-induced aggregation was accompanied by defective release of ADP and ATP. The decrease in total platelet ATP and ADP, the high ATP:ADP ratio in the presence of normal amounts of metabolic adenine nucleotides, and the low content of serotonin associated with abnormal uptake and metabolism of the exogenous amine suggested that the defective platelet function was due to lack of the platelet organelles in which serotonin and nonmetabolic adenine nucleotides are normally stored. Acquired storage pool disease is likely to be related to exposure of circulating platelets to aggregating agents, with their degranulation occurring during disseminated intravascular coagulation.", "contents": "Acquired storage pool disease in platelets during disseminated intravascular coagulation. A patient with clinical and laboratory evidence of disseminated intravascular coagulation associated with deep-vein thrombosis and pulmonary embolism developed a qualitative platelet abnormality characterized by a defective release reaction. Second-phase aggregation induced by ADP and adrenaline was impaired, and reduced collagen-induced aggregation was accompanied by defective release of ADP and ATP. The decrease in total platelet ATP and ADP, the high ATP:ADP ratio in the presence of normal amounts of metabolic adenine nucleotides, and the low content of serotonin associated with abnormal uptake and metabolism of the exogenous amine suggested that the defective platelet function was due to lack of the platelet organelles in which serotonin and nonmetabolic adenine nucleotides are normally stored. Acquired storage pool disease is likely to be related to exposure of circulating platelets to aggregating agents, with their degranulation occurring during disseminated intravascular coagulation.", "PMID": 963291}
{"id": "pubmed23n0032_5501", "title": "A platelet abnormality in the Chediak-Higashi syndrome of man.", "content": "Platelets from two probands homozygous for the Chediak-Higashi syndrome have approximately 10% of the normal number of serotonin-containing dense bodies as visualized electron microscopically in air-dried whole mounts. Since transport of serotonin across the platelet plasma membrane proceeds at a normal rate, and the few dense bodies present appear to store normal amounts of serotonin, the absence of dense bodies may account for the low platelet serotonin values found in these patients.", "contents": "A platelet abnormality in the Chediak-Higashi syndrome of man. Platelets from two probands homozygous for the Chediak-Higashi syndrome have approximately 10% of the normal number of serotonin-containing dense bodies as visualized electron microscopically in air-dried whole mounts. Since transport of serotonin across the platelet plasma membrane proceeds at a normal rate, and the few dense bodies present appear to store normal amounts of serotonin, the absence of dense bodies may account for the low platelet serotonin values found in these patients.", "PMID": 963292}
{"id": "pubmed23n0032_5502", "title": "Immunologic characterization of canine factor VIII.", "content": "Canine factor VIII (FVIII) preparations isolated from cryoprecipitates by gel chromatography were pooled to provide one batch of antigen for simultaneous immunization of two rabbits and a goat. The goat and rabbit antisera had similar FVIII-neutralizing titers, but the latter had seven to ten times more precipitating titer for FVIII-related antigen (FVIII-RA). Absorption with material low in FVIII had little effect on the precipitating titer of the rabbit antibody, but it abolished the precipitating capacity of the goat antibody and caused a 20% reduction in the neutralizing titer of both antisera. Results obtained in the Laurell assay with the two different antisera were similar. This finding was true whether the FVIII-RA levels were reduced, normal, or elevated, as well as for heat-treated and frozen-thawed plasmas. Both antisera were neutralized by the same canine plasma to a similar extent. Analysis of FVIII concentrates by crossed immunoelectrophoresis suggested that canine FVIII-RA was heterogeneous, with slow-and fast-migrating components. The presence of more than one antigenic site on the FVIII complex was also supported by the disparity between the FVIII-neutralizing and -precipitating titers of goat antiserum and by the demonstration that FVIII-RA, FVIII-neutralizing antigen, and procoagulant activity varied independently.", "contents": "Immunologic characterization of canine factor VIII. Canine factor VIII (FVIII) preparations isolated from cryoprecipitates by gel chromatography were pooled to provide one batch of antigen for simultaneous immunization of two rabbits and a goat. The goat and rabbit antisera had similar FVIII-neutralizing titers, but the latter had seven to ten times more precipitating titer for FVIII-related antigen (FVIII-RA). Absorption with material low in FVIII had little effect on the precipitating titer of the rabbit antibody, but it abolished the precipitating capacity of the goat antibody and caused a 20% reduction in the neutralizing titer of both antisera. Results obtained in the Laurell assay with the two different antisera were similar. This finding was true whether the FVIII-RA levels were reduced, normal, or elevated, as well as for heat-treated and frozen-thawed plasmas. Both antisera were neutralized by the same canine plasma to a similar extent. Analysis of FVIII concentrates by crossed immunoelectrophoresis suggested that canine FVIII-RA was heterogeneous, with slow-and fast-migrating components. The presence of more than one antigenic site on the FVIII complex was also supported by the disparity between the FVIII-neutralizing and -precipitating titers of goat antiserum and by the demonstration that FVIII-RA, FVIII-neutralizing antigen, and procoagulant activity varied independently.", "PMID": 963293}
{"id": "pubmed23n0032_5503", "title": "Relationships between thrombopoiesis and erythropoiesis: with studies of the effects of preparations of thrombopoietin and erythropoietin.", "content": "The effects of administration of partially purified human urinary erythropoietin and rabbit thrombopoietin, and of endogenously produced erythropoietin and thrombopoietin on both red cell and platelet production were examined in mice. Partially purified thrombopoietin was prepared from rabbit plasma by sequential fractionation with ammonium sulfate precipitation, and DEAE and Sephadex G-100 chromatography. Preparations of thrombopoietin and partially purified human urinary erythropoietin (NIH No. H-11-TaLSL) were administered subcutaneously to normal mice, and the rate of incorporation of selenomethionine-75 Se into platelets was measured as an index of thrombopoietic activity of the infused material. Erythropoietin and thrombopoietin were assayed for erythropoietic activity by measuring the rate of appearance of 59Fe in the red cells of posthypoxic polycythemic mice. Preparations containing thrombopoietin had barely measurable erythropoietic activity, and 7 units of partially purified erythropoietin had little thrombopoietic activity. When endogenous levels of erythropoietin were increased by hypoxia, platelet production was not enhanced. Similarly, increased levels of thrombopoietin, induced in response to thrombocytopenia produced by platelet antiserum, did not alter red cell production. These data suggest that physiologically increased levels of thrombopoietin do not stimulate erythropoiesis, and that physiologically increased levels of erythropoietn do not stimulate thrombopoiesis. However, currently available, partially purified preparations of erythropoietin and thrombopoietin may be capable of stimulating both platelet and red cell production if used in sufficient quantities.", "contents": "Relationships between thrombopoiesis and erythropoiesis: with studies of the effects of preparations of thrombopoietin and erythropoietin. The effects of administration of partially purified human urinary erythropoietin and rabbit thrombopoietin, and of endogenously produced erythropoietin and thrombopoietin on both red cell and platelet production were examined in mice. Partially purified thrombopoietin was prepared from rabbit plasma by sequential fractionation with ammonium sulfate precipitation, and DEAE and Sephadex G-100 chromatography. Preparations of thrombopoietin and partially purified human urinary erythropoietin (NIH No. H-11-TaLSL) were administered subcutaneously to normal mice, and the rate of incorporation of selenomethionine-75 Se into platelets was measured as an index of thrombopoietic activity of the infused material. Erythropoietin and thrombopoietin were assayed for erythropoietic activity by measuring the rate of appearance of 59Fe in the red cells of posthypoxic polycythemic mice. Preparations containing thrombopoietin had barely measurable erythropoietic activity, and 7 units of partially purified erythropoietin had little thrombopoietic activity. When endogenous levels of erythropoietin were increased by hypoxia, platelet production was not enhanced. Similarly, increased levels of thrombopoietin, induced in response to thrombocytopenia produced by platelet antiserum, did not alter red cell production. These data suggest that physiologically increased levels of thrombopoietin do not stimulate erythropoiesis, and that physiologically increased levels of erythropoietn do not stimulate thrombopoiesis. However, currently available, partially purified preparations of erythropoietin and thrombopoietin may be capable of stimulating both platelet and red cell production if used in sufficient quantities.", "PMID": 963294}
{"id": "pubmed23n0032_5504", "title": "Duplication of part of the long arm of chromosome 1 in marrow cells of a treated case of myelomatosis.", "content": "In a case of classical myelomatosis treated with melphalan, a clone of cells with a chromosomal abnormality was found in the bone marrow during remission. There was good reason to think that the hemopoietic cells, rather than plasmacytoma cells, were implicated. Although the clone persisted, no evidence of leukemia developed over a period of observation of 2 yr. The anomaly was interpreted as a duplication of part of the long arm of chromosome 1, which appeared to involve the segment q21 to q31.", "contents": "Duplication of part of the long arm of chromosome 1 in marrow cells of a treated case of myelomatosis. In a case of classical myelomatosis treated with melphalan, a clone of cells with a chromosomal abnormality was found in the bone marrow during remission. There was good reason to think that the hemopoietic cells, rather than plasmacytoma cells, were implicated. Although the clone persisted, no evidence of leukemia developed over a period of observation of 2 yr. The anomaly was interpreted as a duplication of part of the long arm of chromosome 1, which appeared to involve the segment q21 to q31.", "PMID": 963295}
{"id": "pubmed23n0032_5505", "title": "Some observations of the hematopoietic status in vivo and in vitro on mice of genotype S1/S1d.", "content": "Studies on the mechanism of anemia in mice of genotype S1/S1d have implicated the hematopoietic stroma (the hematopoietic inductive microenvironment, HIM) rather than hematopoietic stem cells as the site of the defect. Using methylcellulose-supported bone marrow culture systems, we have observed, in addition to classical hematopoietic colonies, the formation of surface associated fibroblastic plaques that could stimulate hematopoietic colony growth. These plaques were hypothesized to be derived from bone marrow stroma precursors. In view of the reported stromal-based defect in S1/S1d mice, studies were initiated, using our culture system, to determine if abnormalities exist in the plaque-forming potentials of these mice. Relative to controls, bone marrow derived from S1/S1d mice exhibited a significant decrease in hematopoietic colonly-forming units in culture, but no differences were apparent in the absolute numbers of fibroblastic plaque-forming units or in the ability of such plaques once derived to stimulate hematopoietic colony growth when overlain with fresh normal bone marrow preparations. Quantitative studies on the bone marrow of the S1/S1d mice revealed a marked reduction in total nucleated cells per femur. The importance of evaluating the results of bone marrow cultures in an absolute (i.e., number of units per femur) rather than a relative (i.e., number of units forming in a constant cell inoculum) term was underlined by these studies.", "contents": "Some observations of the hematopoietic status in vivo and in vitro on mice of genotype S1/S1d. Studies on the mechanism of anemia in mice of genotype S1/S1d have implicated the hematopoietic stroma (the hematopoietic inductive microenvironment, HIM) rather than hematopoietic stem cells as the site of the defect. Using methylcellulose-supported bone marrow culture systems, we have observed, in addition to classical hematopoietic colonies, the formation of surface associated fibroblastic plaques that could stimulate hematopoietic colony growth. These plaques were hypothesized to be derived from bone marrow stroma precursors. In view of the reported stromal-based defect in S1/S1d mice, studies were initiated, using our culture system, to determine if abnormalities exist in the plaque-forming potentials of these mice. Relative to controls, bone marrow derived from S1/S1d mice exhibited a significant decrease in hematopoietic colonly-forming units in culture, but no differences were apparent in the absolute numbers of fibroblastic plaque-forming units or in the ability of such plaques once derived to stimulate hematopoietic colony growth when overlain with fresh normal bone marrow preparations. Quantitative studies on the bone marrow of the S1/S1d mice revealed a marked reduction in total nucleated cells per femur. The importance of evaluating the results of bone marrow cultures in an absolute (i.e., number of units per femur) rather than a relative (i.e., number of units forming in a constant cell inoculum) term was underlined by these studies.", "PMID": 963296}
{"id": "pubmed23n0032_5506", "title": "Synthesis of cobalamin coenzymes by human lymphocytes in vitro and the effect of folates and metabolic inhibitors.", "content": "The uptake of 57Co-cyanocobalamin (CN-Cbl) and its conversion to 5-deoxyadenosylcobalamin (Ado-Cbl), methylcobalamin (Me-Cbl), and hydroxocobalamin (OH-Cbl) has been studied in phytohemagglutinin (PHA)-transformed lymphocytes from normal subjects and patients with patients with pernicious anemia. Uptake and conversion were much greater by PHA-stimulated lymphocytes than by mature non-transformed lymphocytes. In normal cells, uptake of 57Co-CN-Cbl and synthesis of the cobalamin coenzymes were approximately linear between 3 and 48 hr incubation. Ado-Cbl was the major cobalamin formed, and after 72 hr the cells contained about twice as much Ado-Cbl as Me-Cbl. Uptake by lymphocytes from patients with untreated pernicious anemia (PA) was greater than that by normal lymphocytes, but the proportions of Ado-Cbl and Me-Cbl synthesized by each were similar. Folic acid and methyltetrahydrofolate enhanced synthesis of Me-Cbl both in normal and in PA cells, while methotrexate and 5-fluorouracil depressed it. This depression was overcome by 5-formyltetrahydrofolate, suggesting that an uninterrupted folate cycle may play an important role in Me-Cbl synthesis.", "contents": "Synthesis of cobalamin coenzymes by human lymphocytes in vitro and the effect of folates and metabolic inhibitors. The uptake of 57Co-cyanocobalamin (CN-Cbl) and its conversion to 5-deoxyadenosylcobalamin (Ado-Cbl), methylcobalamin (Me-Cbl), and hydroxocobalamin (OH-Cbl) has been studied in phytohemagglutinin (PHA)-transformed lymphocytes from normal subjects and patients with patients with pernicious anemia. Uptake and conversion were much greater by PHA-stimulated lymphocytes than by mature non-transformed lymphocytes. In normal cells, uptake of 57Co-CN-Cbl and synthesis of the cobalamin coenzymes were approximately linear between 3 and 48 hr incubation. Ado-Cbl was the major cobalamin formed, and after 72 hr the cells contained about twice as much Ado-Cbl as Me-Cbl. Uptake by lymphocytes from patients with untreated pernicious anemia (PA) was greater than that by normal lymphocytes, but the proportions of Ado-Cbl and Me-Cbl synthesized by each were similar. Folic acid and methyltetrahydrofolate enhanced synthesis of Me-Cbl both in normal and in PA cells, while methotrexate and 5-fluorouracil depressed it. This depression was overcome by 5-formyltetrahydrofolate, suggesting that an uninterrupted folate cycle may play an important role in Me-Cbl synthesis.", "PMID": 963297}
{"id": "pubmed23n0032_5507", "title": "Detection of carriers of hemophilia by hemorrhagometry.", "content": "Hemorrhagometry measures bleeding time, bleeding intensity and blood loss from a small standardized skin wound. In patients with hemophilia these values are within the normal range when hemorrhagometry is performed at room temperature (24 degrees C). However, when the wound is cooled to 17 degrees C (cold tolerance test), bleeding time is abnormally long in hemophiliacs. Therefore we applied this test to carriers of hemophilia. The cold tolerance test was performed in 16 proven and 6 probable carriers (criteria according to Nilsson). 14 proven and 4 probable carriers showed abnormal bleeding times of 15 min and more. When hemorrhagometry was performed at room temperature, the carriers could not be distinguished from normal control persons. Of 15 sisters and aunts of hemophiliacs without male offspring (potential carriers) 9 had abnormal cold tolerance test findings, in fair agreement with the probability of 0.5 to be expected theoretically. Thus the hemorrhagometry cold tolerance test seems to be helpful in detecting carriers of hemophilia.", "contents": "Detection of carriers of hemophilia by hemorrhagometry. Hemorrhagometry measures bleeding time, bleeding intensity and blood loss from a small standardized skin wound. In patients with hemophilia these values are within the normal range when hemorrhagometry is performed at room temperature (24 degrees C). However, when the wound is cooled to 17 degrees C (cold tolerance test), bleeding time is abnormally long in hemophiliacs. Therefore we applied this test to carriers of hemophilia. The cold tolerance test was performed in 16 proven and 6 probable carriers (criteria according to Nilsson). 14 proven and 4 probable carriers showed abnormal bleeding times of 15 min and more. When hemorrhagometry was performed at room temperature, the carriers could not be distinguished from normal control persons. Of 15 sisters and aunts of hemophiliacs without male offspring (potential carriers) 9 had abnormal cold tolerance test findings, in fair agreement with the probability of 0.5 to be expected theoretically. Thus the hemorrhagometry cold tolerance test seems to be helpful in detecting carriers of hemophilia.", "PMID": 963300}
{"id": "pubmed23n0032_5508", "title": "Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse.", "content": "The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to standardize the technique. The slopes of the erythropoietin and cell dose response curves indicate a greater sensitivity by fetal liver to the hormone than bone marrow or spleen. The proportion of cells in the DNA synthesis phase of the cell cycle, as measured by the CFUe technique, has been estimated by administering hydroxyurea. Two hours after the drug was injected, 89% of fetal liver cells, 71% of bone marrow cells and 81% of spleen cells were found to be in the S-phase.", "contents": "Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse. The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to standardize the technique. The slopes of the erythropoietin and cell dose response curves indicate a greater sensitivity by fetal liver to the hormone than bone marrow or spleen. The proportion of cells in the DNA synthesis phase of the cell cycle, as measured by the CFUe technique, has been estimated by administering hydroxyurea. Two hours after the drug was injected, 89% of fetal liver cells, 71% of bone marrow cells and 81% of spleen cells were found to be in the S-phase.", "PMID": 963301}
{"id": "pubmed23n0032_5509", "title": "Evaluation of the humoral immune response of children with low lead exposure.", "content": "Twelve lead-exposed children, with evidence of metabolic impairment, and seven non-lead exposed children were examined for evidence of impairment of their immunological response. There were no differences between the control group and the lead exposed group with reference to complement levels, immunoglobulins, or anamnestic response to the tetanus toxoid antigen. It remains to be demonstrated whether or not there is deficient response to primary immunization, whether other antigens are more affected by lead, or whether impairment of humoral immune response requires a more serious degree of lead intoxication.", "contents": "Evaluation of the humoral immune response of children with low lead exposure. Twelve lead-exposed children, with evidence of metabolic impairment, and seven non-lead exposed children were examined for evidence of impairment of their immunological response. There were no differences between the control group and the lead exposed group with reference to complement levels, immunoglobulins, or anamnestic response to the tetanus toxoid antigen. It remains to be demonstrated whether or not there is deficient response to primary immunization, whether other antigens are more affected by lead, or whether impairment of humoral immune response requires a more serious degree of lead intoxication.", "PMID": 963305}
{"id": "pubmed23n0032_5510", "title": "Isolation and identification of polychlorinated styrenes in Great Lakes fish.", "content": "Octachlorostyrene was prepared by two independent synthetic routes, and GC retention time data as well as a mass spectrum of the standard were obtained. A comparison of these data with those observed for a component in fish from Lake Huron, Lake Ontario, and the lower Detroit River indicates that octachlorostyrene is present in fish residues from these sources. In addition, mass spectral data indicated the presence of several hepta and hexachlorostyrene isomers in these fish. Further studies should be concerned with quantification of OCS and evaluation of possible sources of these compounds, such as impurities in products produced by exhaustive chlorination or as by-products in the electrolytic production of chlorine.", "contents": "Isolation and identification of polychlorinated styrenes in Great Lakes fish. Octachlorostyrene was prepared by two independent synthetic routes, and GC retention time data as well as a mass spectrum of the standard were obtained. A comparison of these data with those observed for a component in fish from Lake Huron, Lake Ontario, and the lower Detroit River indicates that octachlorostyrene is present in fish residues from these sources. In addition, mass spectral data indicated the presence of several hepta and hexachlorostyrene isomers in these fish. Further studies should be concerned with quantification of OCS and evaluation of possible sources of these compounds, such as impurities in products produced by exhaustive chlorination or as by-products in the electrolytic production of chlorine.", "PMID": 963315}
{"id": "pubmed23n0032_5511", "title": "Organochlorine insecticide residues in ducklings and their dilution by growth.", "content": "Residues of DDT, DDD, DDE, and dieldrin in 7 species of ducklings from Alberta, Canada, were measured. Levels of DDT and metabolites ranged from 0 to 36.48 ppm in fat of 96% of the birds and from 0 to 0.97 ppm im muscle from 67% of the birds. Residue levels of dieldrin ranged from 0 to 2.62 ppm in fat and from 0 to 0.11 ppm in muscle of 43 and 19% of the birds, respectively. Growth dilution was considered to be the most significant factor in reducing the insecticide residue levels in the ducklings. DDT (total DDT, DDE, and DDD) residue levels were decreased with increased weight of pintail (A. acuta), baldpate (Mareca americana), and gadwall (A. strepera) ducklings. Dieldrin used in grasshopper control did not contribute to residue levels in the ducklings. Contamination by DDT was considered to have originated from outside the habitat. No dieldrin or DDT residues were found in ducklings' food sources analysed.", "contents": "Organochlorine insecticide residues in ducklings and their dilution by growth. Residues of DDT, DDD, DDE, and dieldrin in 7 species of ducklings from Alberta, Canada, were measured. Levels of DDT and metabolites ranged from 0 to 36.48 ppm in fat of 96% of the birds and from 0 to 0.97 ppm im muscle from 67% of the birds. Residue levels of dieldrin ranged from 0 to 2.62 ppm in fat and from 0 to 0.11 ppm in muscle of 43 and 19% of the birds, respectively. Growth dilution was considered to be the most significant factor in reducing the insecticide residue levels in the ducklings. DDT (total DDT, DDE, and DDD) residue levels were decreased with increased weight of pintail (A. acuta), baldpate (Mareca americana), and gadwall (A. strepera) ducklings. Dieldrin used in grasshopper control did not contribute to residue levels in the ducklings. Contamination by DDT was considered to have originated from outside the habitat. No dieldrin or DDT residues were found in ducklings' food sources analysed.", "PMID": 963316}
{"id": "pubmed23n0032_5512", "title": "Methoxychlor effects on hepatic storage of vitamin A in rats.", "content": "Sixty Sprague-Dawley rats were fed diets containing 0, 10, 100, 1,000, and 10,000 ppm of methoxychlor for 16 weeks under ad libitum- and restricted-feeding regimens. Methoxychlor at 10,000 ppm was lethal to some rats, reduced food consumption and growth, and increased liver weight relative to body weight. Methoxychlor at 1,000 ppm reduced food consumption and growth of rats fed ad libitum but did not reduce growth of restricted-fed rats. Reduced hepatic storage of vitamin A was detectable when methoxychlor was fed at levels of 100 ppm or higher.", "contents": "Methoxychlor effects on hepatic storage of vitamin A in rats. Sixty Sprague-Dawley rats were fed diets containing 0, 10, 100, 1,000, and 10,000 ppm of methoxychlor for 16 weeks under ad libitum- and restricted-feeding regimens. Methoxychlor at 10,000 ppm was lethal to some rats, reduced food consumption and growth, and increased liver weight relative to body weight. Methoxychlor at 1,000 ppm reduced food consumption and growth of rats fed ad libitum but did not reduce growth of restricted-fed rats. Reduced hepatic storage of vitamin A was detectable when methoxychlor was fed at levels of 100 ppm or higher.", "PMID": 963317}
{"id": "pubmed23n0032_5513", "title": "Effects of daily oral subtoxic doses of a wet lead-based paint on cattle.", "content": "Cattle were dosed daily with a wet lead-based paint at a rate of about 6 mg of lead per kg per day for 60 weeks. The cattle did not exhibit overt symptoms of lead toxicosis. Blood and fecal samples collected during the study, and tissue samples obtained at slaughter of the animals were analyzed for lead by atomic absorption spectrophotometer equipped with a graphite furnace. Levels of lead in whole blood generally did not exceed 0.5 ppm. The residues of lead in feces reached as high as 1000 ppm. Highest residues in tissues were in calcified bone, the kidney cortex, kidney medulla, and then in liver. Residues of lead in muscle, brain, spleen, and bone marrow were similar to residues of lead in the control animal. Calves born to 2 test animals had elevated levels of lead in bone, kidney and liver.", "contents": "Effects of daily oral subtoxic doses of a wet lead-based paint on cattle. Cattle were dosed daily with a wet lead-based paint at a rate of about 6 mg of lead per kg per day for 60 weeks. The cattle did not exhibit overt symptoms of lead toxicosis. Blood and fecal samples collected during the study, and tissue samples obtained at slaughter of the animals were analyzed for lead by atomic absorption spectrophotometer equipped with a graphite furnace. Levels of lead in whole blood generally did not exceed 0.5 ppm. The residues of lead in feces reached as high as 1000 ppm. Highest residues in tissues were in calcified bone, the kidney cortex, kidney medulla, and then in liver. Residues of lead in muscle, brain, spleen, and bone marrow were similar to residues of lead in the control animal. Calves born to 2 test animals had elevated levels of lead in bone, kidney and liver.", "PMID": 963319}
{"id": "pubmed23n0032_5514", "title": "The activity of delta 5-3 beta hydroxysteroid dehydrogenase enzyme in the interrenal tissue of rainbow trout (Salmo gairdneri Richardson) exposed to sublethal concentrations of zinc.", "content": "The effects of exposure of rainbow trout (Salmo gairdneri Richardson) to three sublethal concentrations of zinc (0.248, 0.528 and 1.14 ppm) on delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta HSDH) enzyme activity in the head kidney tissue was investigated. The activity of this enzyme was localized by the reduction of a tetrazolium salt to formazan at the site of activity. Using subjective methods to indicate the degree of activity and indirectly assess the degree of corticosteroid production, the zinc-exposed rainbow trout showed a greater degree of delta 5-3 beta HSDH activity compared to the control fish. This increase in enzyme activity has been associated with the stimulation of the pituitary-interrenal axis by the noxious stress of zinc.", "contents": "The activity of delta 5-3 beta hydroxysteroid dehydrogenase enzyme in the interrenal tissue of rainbow trout (Salmo gairdneri Richardson) exposed to sublethal concentrations of zinc. The effects of exposure of rainbow trout (Salmo gairdneri Richardson) to three sublethal concentrations of zinc (0.248, 0.528 and 1.14 ppm) on delta 5-3 beta-hydroxysteroid dehydrogenase (delta 5-3 beta HSDH) enzyme activity in the head kidney tissue was investigated. The activity of this enzyme was localized by the reduction of a tetrazolium salt to formazan at the site of activity. Using subjective methods to indicate the degree of activity and indirectly assess the degree of corticosteroid production, the zinc-exposed rainbow trout showed a greater degree of delta 5-3 beta HSDH activity compared to the control fish. This increase in enzyme activity has been associated with the stimulation of the pituitary-interrenal axis by the noxious stress of zinc.", "PMID": 963321}
{"id": "pubmed23n0032_5515", "title": "The effect of lead and cadmium on liver, kidney, and brain levels of cadmium, copper, lead, manganese, and zinc, and on erythrocyte ALA-D activity in mice.", "content": "Lead and cadmium were administered intraperitoneally, singly and jointly, to the mice. The levels of cadmium, copper, manganese, lead and zinc were determined in liver, kidney and brain by atomic absorption spectrophotometric technique and delta-aminolevulinic acid dehydratase (ALA-D) activity was determined in erythrocytes. The tissue levels of some of these metals were found significantly altered by cadmium and lead both, but cadmium was found to have no effect on blood on ALA-D activity.", "contents": "The effect of lead and cadmium on liver, kidney, and brain levels of cadmium, copper, lead, manganese, and zinc, and on erythrocyte ALA-D activity in mice. Lead and cadmium were administered intraperitoneally, singly and jointly, to the mice. The levels of cadmium, copper, manganese, lead and zinc were determined in liver, kidney and brain by atomic absorption spectrophotometric technique and delta-aminolevulinic acid dehydratase (ALA-D) activity was determined in erythrocytes. The tissue levels of some of these metals were found significantly altered by cadmium and lead both, but cadmium was found to have no effect on blood on ALA-D activity.", "PMID": 963322}
{"id": "pubmed23n0032_5516", "title": "The toxicity of benomyl, thiophanate-methyl, and BCM to four freshwater organisms.", "content": "The EC50 or LC50 values after a 2-day exposure of the three fungicides benomyl, thiophanate-methyl and BCM, have been determined using four freshwater organisms: Chlorella pyrenoidosa, Daphnia magna, Lebistes reticulatus and Salmo gairdneri. The influence of BCM on the reproductive capacity of Daphnia was also investigated and from these data provisional ecological limit was established.", "contents": "The toxicity of benomyl, thiophanate-methyl, and BCM to four freshwater organisms. The EC50 or LC50 values after a 2-day exposure of the three fungicides benomyl, thiophanate-methyl and BCM, have been determined using four freshwater organisms: Chlorella pyrenoidosa, Daphnia magna, Lebistes reticulatus and Salmo gairdneri. The influence of BCM on the reproductive capacity of Daphnia was also investigated and from these data provisional ecological limit was established.", "PMID": 963326}
{"id": "pubmed23n0032_5517", "title": "Tolerance pattern of the anorexigenic action of amphetamines, fenfluramine, phenmetrazine and diethylpropion in rats.", "content": "The tolerance pattern to anorectic drugs was studied in starved rats by measuring two consecutive 2 h food intakes. 2 There was a reduction in the first 2 h food intake with development of complete tolerance after fenfluramine and phenmetrazine, and of partial tolerance after amphetamine, (+)-amphetamine and diethylpropion. 3 During the second 2 h intake, the anorectic effect was transient after fenfluramine and diethylpropion; while there was an absolute increase in the intake after amphetamine and (+)-amphetamine. 4 A pair-feeding experiment revealed that the increase in the second 2 h food intake was not a direct effect of the drug but a consequence of the deficit in food intake during the preceding 2 hours. 5 There was an overall correlation between the food and water intake. 6 A significant loss in body weight was observed after amphetamine, fenfluramine and phenmetrazine but not after (+)-amphetamine or diethylpropion. 7 The results indicate that so-called tolerance to the anorexigenic effect of drugs is apparent rather than real and that the duration of food access is a determining factor. The body weight changes may be brought about by the metabolic effects of these drugs rather than their effect on food and water intake.", "contents": "Tolerance pattern of the anorexigenic action of amphetamines, fenfluramine, phenmetrazine and diethylpropion in rats. The tolerance pattern to anorectic drugs was studied in starved rats by measuring two consecutive 2 h food intakes. 2 There was a reduction in the first 2 h food intake with development of complete tolerance after fenfluramine and phenmetrazine, and of partial tolerance after amphetamine, (+)-amphetamine and diethylpropion. 3 During the second 2 h intake, the anorectic effect was transient after fenfluramine and diethylpropion; while there was an absolute increase in the intake after amphetamine and (+)-amphetamine. 4 A pair-feeding experiment revealed that the increase in the second 2 h food intake was not a direct effect of the drug but a consequence of the deficit in food intake during the preceding 2 hours. 5 There was an overall correlation between the food and water intake. 6 A significant loss in body weight was observed after amphetamine, fenfluramine and phenmetrazine but not after (+)-amphetamine or diethylpropion. 7 The results indicate that so-called tolerance to the anorexigenic effect of drugs is apparent rather than real and that the duration of food access is a determining factor. The body weight changes may be brought about by the metabolic effects of these drugs rather than their effect on food and water intake.", "PMID": 963336}
{"id": "pubmed23n0032_5518", "title": "Oestrogenic, anti-oestrogenic and fertility effects of some triphenylethanes and triphenylethylenes related to ethamoxytriphetol (MER 25).", "content": "Five triphenylethylenes, a triphenylethane and a triphenylethanol, carrying methyl substituents ortho to one or both of the ring oxygen functions, have been examined for oestrogenic, and antioestrogenic activity in mice, and three of the compounds, alpha-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-alpha-phenyl-beta-4-methoxyphenyl-ethanol (IV), alpha'-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-4-methoxy-bibenzyl (V) and alpha'-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-4-methoxy-stilbene (VI), were tested for their effects on fertility in mice. 2 Orthomethylation reduces oestrogenic and/or anti-oestrogenic activity compared with the reported activities of non-methylated analogues. 3 The anti-oestrogenic ethamoxytriphetol (MER 25) reduced fertility in mice whereas its inactive dimethylated analogue (IV) was ineffective. The weakly active anti-oestrogens, V and VI, did not affect fertility in mice.", "contents": "Oestrogenic, anti-oestrogenic and fertility effects of some triphenylethanes and triphenylethylenes related to ethamoxytriphetol (MER 25). Five triphenylethylenes, a triphenylethane and a triphenylethanol, carrying methyl substituents ortho to one or both of the ring oxygen functions, have been examined for oestrogenic, and antioestrogenic activity in mice, and three of the compounds, alpha-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-alpha-phenyl-beta-4-methoxyphenyl-ethanol (IV), alpha'-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-4-methoxy-bibenzyl (V) and alpha'-[4-(beta-diethylaminoethoxy)-3, 5-xylyl]-4-methoxy-stilbene (VI), were tested for their effects on fertility in mice. 2 Orthomethylation reduces oestrogenic and/or anti-oestrogenic activity compared with the reported activities of non-methylated analogues. 3 The anti-oestrogenic ethamoxytriphetol (MER 25) reduced fertility in mice whereas its inactive dimethylated analogue (IV) was ineffective. The weakly active anti-oestrogens, V and VI, did not affect fertility in mice.", "PMID": 963337}
{"id": "pubmed23n0032_5519", "title": "Dual effects of aspirin in guinea-pig lungs.", "content": "Dual effects of aspirin were demonstrated in guinea-pig lungs: (a) aspirin (3.3 mg/kg i.v.) antagonized bronchoconstriction induced by slow reacting substance of anaphylaxis (SRS-A); (b) aspirin produced bronchoconstriction when injected in the presence of propranolol into guinea-pigs in vivo at 330 mg/kg, or into guinea-pig isolated lungs in vitro as a 4% solution (40 mg/ml). 2 The severity of bronchoconstriction following administration of aspirin was directly related to the degree of beta-adrenoceptor blockade and to the age of the guinea-pigs. Aspirin-induced bronchoconstriction was prevented in vivo and in vitro by atropine and it could be reversed in vivo by atropine. Aspirin-induced bronchoconstriction was not inhibited by vagotomy or phenoxybenzamine. 3 These data suggest that the mechanism involved in aspirin-induced bronchoconstriction may be local cholinergic stimulation and that reduced beta-adrenergic drive may be a predisposing factor.", "contents": "Dual effects of aspirin in guinea-pig lungs. Dual effects of aspirin were demonstrated in guinea-pig lungs: (a) aspirin (3.3 mg/kg i.v.) antagonized bronchoconstriction induced by slow reacting substance of anaphylaxis (SRS-A); (b) aspirin produced bronchoconstriction when injected in the presence of propranolol into guinea-pigs in vivo at 330 mg/kg, or into guinea-pig isolated lungs in vitro as a 4% solution (40 mg/ml). 2 The severity of bronchoconstriction following administration of aspirin was directly related to the degree of beta-adrenoceptor blockade and to the age of the guinea-pigs. Aspirin-induced bronchoconstriction was prevented in vivo and in vitro by atropine and it could be reversed in vivo by atropine. Aspirin-induced bronchoconstriction was not inhibited by vagotomy or phenoxybenzamine. 3 These data suggest that the mechanism involved in aspirin-induced bronchoconstriction may be local cholinergic stimulation and that reduced beta-adrenergic drive may be a predisposing factor.", "PMID": 963338}
{"id": "pubmed23n0032_5520", "title": "The ionization of phenolic amines, including apomorphine, dopamine and catecholamines and an assessment of zwitterion constants.", "content": "The dissociation constants of many phenolic amines, including benzylamines, phenethylamines, phenylethanolamines, phenylpropylamines, catecholamines, and apomorphine have been measured by potentiometric titration at 25 degrees C. Measurements have also been made with many of their methoxy derivatives and with series of phenolic quaternary ammonium salts. Some compounds were also studied at 37 degrees C. 2 Usually at least five titrations were made with each compound and Debye--H\u00fcckel theory was applied to convert concentrations to activities but the estimates of pKa were not constant and found to increase with increasing concentration. The range studied was usually 5-15 mM and a least-squares line-fit, based on the empirical assumption that pKa varies with (concentration)1/2, has been used to calculate values for 10 mM solutions and to extrapolate to infinite dilution and to 100 mM. The dependence of pKa on concentration was much less at 37 degrees C than at 25 degrees C. 3 At 37 degrees C the pKa values of many biologically interesting compounds in the group, dopamine, noradrenaline, adrenaline and isoprenaline, coryneine (the trimethylammonium derivative of dopamine) and apomorphine are within 1 log unit of physiological pH, indicating the presence of a significant proportion of either the zwitterion or of the uncharged phenolic amine. 4 Zwitterion constants have been estimated from the pKa values of the phenolic amines and those of their methoxy and quaternary trimethylammonium analogues. Zwitterion formation does not appear to be associated with activity at alpha-adrenoceptors and probably not with activity at beta-receptors. The active species seems likely to contain the unionised phenolic group but at dopamine receptors this may be in the uncharged phenolic amine rather than in the phenolic ammonium salt.", "contents": "The ionization of phenolic amines, including apomorphine, dopamine and catecholamines and an assessment of zwitterion constants. The dissociation constants of many phenolic amines, including benzylamines, phenethylamines, phenylethanolamines, phenylpropylamines, catecholamines, and apomorphine have been measured by potentiometric titration at 25 degrees C. Measurements have also been made with many of their methoxy derivatives and with series of phenolic quaternary ammonium salts. Some compounds were also studied at 37 degrees C. 2 Usually at least five titrations were made with each compound and Debye--H\u00fcckel theory was applied to convert concentrations to activities but the estimates of pKa were not constant and found to increase with increasing concentration. The range studied was usually 5-15 mM and a least-squares line-fit, based on the empirical assumption that pKa varies with (concentration)1/2, has been used to calculate values for 10 mM solutions and to extrapolate to infinite dilution and to 100 mM. The dependence of pKa on concentration was much less at 37 degrees C than at 25 degrees C. 3 At 37 degrees C the pKa values of many biologically interesting compounds in the group, dopamine, noradrenaline, adrenaline and isoprenaline, coryneine (the trimethylammonium derivative of dopamine) and apomorphine are within 1 log unit of physiological pH, indicating the presence of a significant proportion of either the zwitterion or of the uncharged phenolic amine. 4 Zwitterion constants have been estimated from the pKa values of the phenolic amines and those of their methoxy and quaternary trimethylammonium analogues. Zwitterion formation does not appear to be associated with activity at alpha-adrenoceptors and probably not with activity at beta-receptors. The active species seems likely to contain the unionised phenolic group but at dopamine receptors this may be in the uncharged phenolic amine rather than in the phenolic ammonium salt.", "PMID": 963339}
{"id": "pubmed23n0032_5521", "title": "Calcium depletion of synaptosomes after morphine treatment.", "content": "It was confirmed that morphine administration in vivo produced a selective decrease in the calcium content of synaptosomal fractions prepared from rat cerebral cortices. This effect was prevented by naloxone. 2 After morphine or naloxone, there were no changes in the sodium, potassium and magnesium contents of any of the seven subcellular fractions tested. 3 Since the selective calcium loss was found to occur from fractions similar to those reported to exhibit opiate receptor binding, it is concluded that calcium may play a role in the action of narcotic agonist drugs.", "contents": "Calcium depletion of synaptosomes after morphine treatment. It was confirmed that morphine administration in vivo produced a selective decrease in the calcium content of synaptosomal fractions prepared from rat cerebral cortices. This effect was prevented by naloxone. 2 After morphine or naloxone, there were no changes in the sodium, potassium and magnesium contents of any of the seven subcellular fractions tested. 3 Since the selective calcium loss was found to occur from fractions similar to those reported to exhibit opiate receptor binding, it is concluded that calcium may play a role in the action of narcotic agonist drugs.", "PMID": 963340}
{"id": "pubmed23n0032_5522", "title": "Effect of ionizing radiation on prostaglandin-like activity in tissues.", "content": "One to 7 days after whole body exposure of mice to a single dose of 700 R of x-rays, little or no change was detected in prostaglandin-like activity in the brain, blood and seminal vesicles. Slight increases in intestinal and renal tissue were not significant. In the lung, mean activity rose from 62 ng/g to a transient peak of 145 ng/g wet weight on the fourth day (P less than 0.05). In the spleen, mean levels rose steadily from 13.2 ng/g to 259 ng/g on the fourth day (P less than 0.01), and were still 184.4 ng/g on the seventh day. 2 Prostaglandin-like activity was measured 4 days after single doses of 200-700 R. In the lung, a significant rise was produced by 600 and 700 R, and in the spleen by 200-700 R. 3 Thin layer chromatography showed that part of the prostaglandin-like activity in spleen extracts had RF similar to that of [3H]-prostaglandin E1, and part to that of [3H]-prostaglandin F2alpha. 4 Splenic tissue from mice exposed to 700 R four days earlier, inactivated prostaglandin E1 less potently than did tissue from non-irradiated mice.", "contents": "Effect of ionizing radiation on prostaglandin-like activity in tissues. One to 7 days after whole body exposure of mice to a single dose of 700 R of x-rays, little or no change was detected in prostaglandin-like activity in the brain, blood and seminal vesicles. Slight increases in intestinal and renal tissue were not significant. In the lung, mean activity rose from 62 ng/g to a transient peak of 145 ng/g wet weight on the fourth day (P less than 0.05). In the spleen, mean levels rose steadily from 13.2 ng/g to 259 ng/g on the fourth day (P less than 0.01), and were still 184.4 ng/g on the seventh day. 2 Prostaglandin-like activity was measured 4 days after single doses of 200-700 R. In the lung, a significant rise was produced by 600 and 700 R, and in the spleen by 200-700 R. 3 Thin layer chromatography showed that part of the prostaglandin-like activity in spleen extracts had RF similar to that of [3H]-prostaglandin E1, and part to that of [3H]-prostaglandin F2alpha. 4 Splenic tissue from mice exposed to 700 R four days earlier, inactivated prostaglandin E1 less potently than did tissue from non-irradiated mice.", "PMID": 963341}
{"id": "pubmed23n0032_5523", "title": "Interactions of isoprenaline and prostaglandin E2 with respect to myocardial contractile force, coronary vascular resistance and myocardial oxygen consumption in guinea-pig isolated hearts.", "content": "Left ventricular pressure (LVP), left ventricular pressure derivative (LV dp/dtmax), coronary vascular resistance (CVR) and myocardial oxygen consumption (Qo2) were measured simultaneously in isolated, electrically driven hearts of guinea-pigs at constant perfusion rate. 2 LVP, LV dp/dtmax, CVR and Qo2 were greatly decreased by either the addition of prostaglandin E2 (50 ng/ml) to the perfusion fluid or pretreatment of the animals with reserpine. 3 Isoprenaline (0.5 nM to 100 nM) induced increases in LVP, LV dp/dtmax and Qo2. In the presence of prostaglandin E2, there was a parallel shift of the isoprenaline concentration-response curve for LVP and LV dp/dtmax. This effect was not seen, after the animals had been treated with reserpine. 4 Qo2 was also decreased by prostaglandin E2 only in non-reserpine treated animals. 5 CVR was diminished by isoprenaline in the untreated group. However, there was increase in CVR, when isoprenaline was added to either prostaglandin E2 or reserpine-pretreated hearts which was enhanced in the reserpine plus prostaglandin E2-treated group (P less than 0.01). 6 The results give evidence for different actions of prostaglandin E2 on isoprenaline concentration-response curves for LVP, LV dp/dtmax and CVR.", "contents": "Interactions of isoprenaline and prostaglandin E2 with respect to myocardial contractile force, coronary vascular resistance and myocardial oxygen consumption in guinea-pig isolated hearts. Left ventricular pressure (LVP), left ventricular pressure derivative (LV dp/dtmax), coronary vascular resistance (CVR) and myocardial oxygen consumption (Qo2) were measured simultaneously in isolated, electrically driven hearts of guinea-pigs at constant perfusion rate. 2 LVP, LV dp/dtmax, CVR and Qo2 were greatly decreased by either the addition of prostaglandin E2 (50 ng/ml) to the perfusion fluid or pretreatment of the animals with reserpine. 3 Isoprenaline (0.5 nM to 100 nM) induced increases in LVP, LV dp/dtmax and Qo2. In the presence of prostaglandin E2, there was a parallel shift of the isoprenaline concentration-response curve for LVP and LV dp/dtmax. This effect was not seen, after the animals had been treated with reserpine. 4 Qo2 was also decreased by prostaglandin E2 only in non-reserpine treated animals. 5 CVR was diminished by isoprenaline in the untreated group. However, there was increase in CVR, when isoprenaline was added to either prostaglandin E2 or reserpine-pretreated hearts which was enhanced in the reserpine plus prostaglandin E2-treated group (P less than 0.01). 6 The results give evidence for different actions of prostaglandin E2 on isoprenaline concentration-response curves for LVP, LV dp/dtmax and CVR.", "PMID": 963342}
{"id": "pubmed23n0032_5524", "title": "The effects of morphine on the release of noradrenaline from the mouse vas deferens.", "content": "Electrical field stimulation of the mouse vas deferens (TO and C57/BL strains) caused the release of noradrenaline into the bathing medium. 2 Phenoxybenzamine (30 muM) or phentolamine (36 muM) plus cocaine (13 muM) caused a considerable increase in the noradrenaline output. 3 In the vasa deferentia from TO mice the output per pulse of noradrenaline was constant at frequencies of stimulation from 0.5 to 15 Hz whereas in the vasa deferentia from C57/BL mice the output per pulse of noradrenaline increased two-fold from 1.5 to 15 Hz. 4 Morphine (2 muM) inhibited the contractions of the vasa deferentia from TO mice. This effect was greater at low (0.1-1 Hz) than at high (10 Hz) frequencies of stimulation. Morphine (2 muM) did not inhibit the response of the tissue to exogenous noradrenaline. 5 Morphine (1 muM) reduced the noradrenaline output from the vasa deferentia of TO mice stimulated at 1.5 Hz but did not reduce the noradrenaline output at 15 Hz. At 1.5 Hz the reduction of noradrenaline output was reversed by naloxone (0.05 muM). 6 Morphine (5 muM) did not inhibit the uptake of [3H]-noradrenaline into the vasa deferentia from TO mice. 7 Only in high concentrations (ID50 30.88 muM) did morphine inhibit the contractions of the vasa deferentia from C57/BL mice. 8 Normorphine (100 muM) did not reduce the noradrenaline output from vasa deferentia of C57/BL mice.", "contents": "The effects of morphine on the release of noradrenaline from the mouse vas deferens. Electrical field stimulation of the mouse vas deferens (TO and C57/BL strains) caused the release of noradrenaline into the bathing medium. 2 Phenoxybenzamine (30 muM) or phentolamine (36 muM) plus cocaine (13 muM) caused a considerable increase in the noradrenaline output. 3 In the vasa deferentia from TO mice the output per pulse of noradrenaline was constant at frequencies of stimulation from 0.5 to 15 Hz whereas in the vasa deferentia from C57/BL mice the output per pulse of noradrenaline increased two-fold from 1.5 to 15 Hz. 4 Morphine (2 muM) inhibited the contractions of the vasa deferentia from TO mice. This effect was greater at low (0.1-1 Hz) than at high (10 Hz) frequencies of stimulation. Morphine (2 muM) did not inhibit the response of the tissue to exogenous noradrenaline. 5 Morphine (1 muM) reduced the noradrenaline output from the vasa deferentia of TO mice stimulated at 1.5 Hz but did not reduce the noradrenaline output at 15 Hz. At 1.5 Hz the reduction of noradrenaline output was reversed by naloxone (0.05 muM). 6 Morphine (5 muM) did not inhibit the uptake of [3H]-noradrenaline into the vasa deferentia from TO mice. 7 Only in high concentrations (ID50 30.88 muM) did morphine inhibit the contractions of the vasa deferentia from C57/BL mice. 8 Normorphine (100 muM) did not reduce the noradrenaline output from vasa deferentia of C57/BL mice.", "PMID": 963343}
{"id": "pubmed23n0032_5525", "title": "The vasodilator actions of isoprenaline, histamine, prostaglandin E2, glucagon and secretin on the hepatic arterial vascular bed of the dog.", "content": "The sympathetically-innervated arterial vascular bed of the dog's liver was perfused from a femoral artery. Arterial blood flow and perfusion pressure were measured continuously, and the hepatic arterial vascular resistance calculated. The preparation provided a means of assessing hepatic arterial vasodilatation quantitatively. 2 Isoprenaline, histamine, prostaglandin E2, glucagon and secretin were injected intra-arterially and all evoked dose-dependent vasodilatation of the hepatic arterial vascular bed. 3 The maximum reduction in the calculated hepatic arterial vascular resistance of 37-38% was the same for each of the five substances. 4 Comparisons on a weight basis revealed that prostaglandin E2 was the most potent, followed in potency order by secretin, isoprenaline, histamine and glucagon. 5 Comparisons on a molar basis showed that secretin and prostaglandin E3 were intrinsically considerably more potent than isoprenaline, histamine or glucagon. 6 The onset of the vasodilatator responses to secretin, isoprenaline, histamine and prostaglandin E2, was rapid, and the duration of their actions was brief. 7 The onset of the vasodilator effects of glucagon was slow and its duration of action very prolonged. 8 The implications of these observations with respect to the physiological control of the hepatic arterial vascular bed of the dog are discussed.", "contents": "The vasodilator actions of isoprenaline, histamine, prostaglandin E2, glucagon and secretin on the hepatic arterial vascular bed of the dog. The sympathetically-innervated arterial vascular bed of the dog's liver was perfused from a femoral artery. Arterial blood flow and perfusion pressure were measured continuously, and the hepatic arterial vascular resistance calculated. The preparation provided a means of assessing hepatic arterial vasodilatation quantitatively. 2 Isoprenaline, histamine, prostaglandin E2, glucagon and secretin were injected intra-arterially and all evoked dose-dependent vasodilatation of the hepatic arterial vascular bed. 3 The maximum reduction in the calculated hepatic arterial vascular resistance of 37-38% was the same for each of the five substances. 4 Comparisons on a weight basis revealed that prostaglandin E2 was the most potent, followed in potency order by secretin, isoprenaline, histamine and glucagon. 5 Comparisons on a molar basis showed that secretin and prostaglandin E3 were intrinsically considerably more potent than isoprenaline, histamine or glucagon. 6 The onset of the vasodilatator responses to secretin, isoprenaline, histamine and prostaglandin E2, was rapid, and the duration of their actions was brief. 7 The onset of the vasodilator effects of glucagon was slow and its duration of action very prolonged. 8 The implications of these observations with respect to the physiological control of the hepatic arterial vascular bed of the dog are discussed.", "PMID": 963344}
{"id": "pubmed23n0032_5526", "title": "A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology.", "content": "A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.", "contents": "A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology. A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.", "PMID": 963345}
{"id": "pubmed23n0032_5527", "title": "Noradrenaline modulation of the responses of the cerebellar Purkinje cell to afferent synaptic activity.", "content": "Noradrenaline, applied by microiontophoresis to rat cerebellar Purkinje neurones, selectively depressed spontaneous neuronal discharge. Simple spike and complex spike excitations, evoked by stimulation of the mossy and climbing fibres, were relatively preserved during the inhibition of spontaneous activity, and the number of full-sized action potentials in the complex spike increased. Inhibition mediated by the basket and stellate cells was augmented. Thus, relative to the change in spontaneous activity, noradrenaline increased the responsiveness of the Purkinje cell to afferent input.", "contents": "Noradrenaline modulation of the responses of the cerebellar Purkinje cell to afferent synaptic activity. Noradrenaline, applied by microiontophoresis to rat cerebellar Purkinje neurones, selectively depressed spontaneous neuronal discharge. Simple spike and complex spike excitations, evoked by stimulation of the mossy and climbing fibres, were relatively preserved during the inhibition of spontaneous activity, and the number of full-sized action potentials in the complex spike increased. Inhibition mediated by the basket and stellate cells was augmented. Thus, relative to the change in spontaneous activity, noradrenaline increased the responsiveness of the Purkinje cell to afferent input.", "PMID": 963346}
{"id": "pubmed23n0032_5528", "title": "The influence of family and social factors on the course of psychiatric illness. A comparison of schizophrenic and depressed neurotic patients.", "content": "This study is a replication and extension of past work carried out by Brown, Birley and Wing (1972) concerning the influence of family life on the course of schizophrenia. In the original research the index of emotion expressed by a key relative about the patient at the time of key admission proved to be the best single predictor of symptomatic relapse in the nine months after discharge from hospital. In the present study this main finding of Brown et al has been replicated for two clinically different groups of psychiatric patients. The expressed emotion of the relative again seems to be associated with relapse independently of all other social and clinical factors investigated. In addition, important additive effects between various social influences and pharmacological treatments have been revealed which make it possible to predict relapse patterns in schizophrenia with considerable precision. The patterns of these relationships with relapse are different for the two clinical groups studied, patients with schizophrenic psychosis and with depressive neurosis.", "contents": "The influence of family and social factors on the course of psychiatric illness. A comparison of schizophrenic and depressed neurotic patients. This study is a replication and extension of past work carried out by Brown, Birley and Wing (1972) concerning the influence of family life on the course of schizophrenia. In the original research the index of emotion expressed by a key relative about the patient at the time of key admission proved to be the best single predictor of symptomatic relapse in the nine months after discharge from hospital. In the present study this main finding of Brown et al has been replicated for two clinically different groups of psychiatric patients. The expressed emotion of the relative again seems to be associated with relapse independently of all other social and clinical factors investigated. In addition, important additive effects between various social influences and pharmacological treatments have been revealed which make it possible to predict relapse patterns in schizophrenia with considerable precision. The patterns of these relationships with relapse are different for the two clinical groups studied, patients with schizophrenic psychosis and with depressive neurosis.", "PMID": 963348}
{"id": "pubmed23n0032_5529", "title": "Rationing out-patients: a defence of the waiting list.", "content": "New psychiatric out-patients were offered immediate or delayed appointments (average delay 12 weeks) in random order. The two groups thus formed, comprising 234 patients, were shown to be comparable in some important respects. Immediate appointments were taken up significantly more frequently than delayed appointments, and 12 per cent of immediate and 22 per cent of delayed patients did not receive any psychiatric service. No evidence was found that delay increased other medical services supplied. Non-attenders were presumed to suffer from \"neurosis\" and \"personality problem\". Initial attenders used more services than those who failed to keep their first appointment but came later. A waiting list is proposed as a screening device to limit out-patient referrals.", "contents": "Rationing out-patients: a defence of the waiting list. New psychiatric out-patients were offered immediate or delayed appointments (average delay 12 weeks) in random order. The two groups thus formed, comprising 234 patients, were shown to be comparable in some important respects. Immediate appointments were taken up significantly more frequently than delayed appointments, and 12 per cent of immediate and 22 per cent of delayed patients did not receive any psychiatric service. No evidence was found that delay increased other medical services supplied. Non-attenders were presumed to suffer from \"neurosis\" and \"personality problem\". Initial attenders used more services than those who failed to keep their first appointment but came later. A waiting list is proposed as a screening device to limit out-patient referrals.", "PMID": 963349}
{"id": "pubmed23n0032_5530", "title": "Some possible childhood indicators of adult schizophrenia inferred from children of schizophrenics.", "content": "Prospective developmental data on 33 children of consensus diagnosed schizophrenic parents were compared to control samples of children of other psychiatric patients and children of normal parents. Contrary to several reports the children of schizophrenics, observed from birth to age seven, were remarkably normal on a host of pregnancy and delivery variables, neurological examinations, physical growth measures, and psychological tests. However, a combination of three indicators characterized five of the 116 children, and all five were offspring of schizophrenics (17 per cent of the high risk sample), thus matching the expected lifetime risk for schizophrenia previously observed in such samples. Case histories show that all five children have enduring patterns of maladjustment and exhibit behaviours often reported in the premorbid histories of schizophrenics. Most have family histories heavily loaded with schizophrenia. In terms of a diathesis-stress model, these five children appear especially vulnerable to future schizophrenia in the absence of rational methods of prevention.", "contents": "Some possible childhood indicators of adult schizophrenia inferred from children of schizophrenics. Prospective developmental data on 33 children of consensus diagnosed schizophrenic parents were compared to control samples of children of other psychiatric patients and children of normal parents. Contrary to several reports the children of schizophrenics, observed from birth to age seven, were remarkably normal on a host of pregnancy and delivery variables, neurological examinations, physical growth measures, and psychological tests. However, a combination of three indicators characterized five of the 116 children, and all five were offspring of schizophrenics (17 per cent of the high risk sample), thus matching the expected lifetime risk for schizophrenia previously observed in such samples. Case histories show that all five children have enduring patterns of maladjustment and exhibit behaviours often reported in the premorbid histories of schizophrenics. Most have family histories heavily loaded with schizophrenia. In terms of a diathesis-stress model, these five children appear especially vulnerable to future schizophrenia in the absence of rational methods of prevention.", "PMID": 963350}
{"id": "pubmed23n0032_5531", "title": "Trainees' competence in psychiatric case writing.", "content": "An assessment was made of 100 case records written by junior medical staff working in a psychiatric teaching hospital. Thirty-nine items were rated, and considerable gaps were found in the records. The Mental State was more poorly recorded than the History; the Formulation and Summary were more satisfactory. Possible reasons for the findings are discussed, including methodological difficulties, administrative considerations, and attitudes of junior staff. The implications for the training and assessment of junior psychiatrists are explored.", "contents": "Trainees' competence in psychiatric case writing. An assessment was made of 100 case records written by junior medical staff working in a psychiatric teaching hospital. Thirty-nine items were rated, and considerable gaps were found in the records. The Mental State was more poorly recorded than the History; the Formulation and Summary were more satisfactory. Possible reasons for the findings are discussed, including methodological difficulties, administrative considerations, and attitudes of junior staff. The implications for the training and assessment of junior psychiatrists are explored.", "PMID": 963351}
{"id": "pubmed23n0032_5532", "title": "The differentiation of paranoid from affective psychoses by patients' premorbid characteristics.", "content": "Various premorbid characteristics of a group of patients aged 50 or over suffering from paranoid psychoses (N = 54) were compared with those of patients of similar age suffering from affective psychoses (N = 57). (The diagnoses were those of the consultants.) The presence of deafness was investigated in the manner described by Cooper et al(1974). Using the discriminant function method, the diagnostic groups were highly significantly differentiated by six independent variables, which in combination predicted 40 per cent of the variance. These were: a 'schizoid personality' factor, the number of surviving children, precipitating events, deafness, family history and social class.", "contents": "The differentiation of paranoid from affective psychoses by patients' premorbid characteristics. Various premorbid characteristics of a group of patients aged 50 or over suffering from paranoid psychoses (N = 54) were compared with those of patients of similar age suffering from affective psychoses (N = 57). (The diagnoses were those of the consultants.) The presence of deafness was investigated in the manner described by Cooper et al(1974). Using the discriminant function method, the diagnostic groups were highly significantly differentiated by six independent variables, which in combination predicted 40 per cent of the variance. These were: a 'schizoid personality' factor, the number of surviving children, precipitating events, deafness, family history and social class.", "PMID": 963354}
{"id": "pubmed23n0032_5533", "title": "Deafness and psychiatric illness.", "content": "Review of the literature concerning the relationship between deafness and psychiatric disorder reveals differences in the pattern of illness depending on the severity of deafness and the age of onset. In particular, the prevalence of schizophrenia in the prelingually deaf is similar to that found in the normal population, whereas the hard of hearing are over-represented among samples of patients suffering from paranoid psychoses in later life. Possible modes of action of long-standing hearing loss in the aetiology of paranoid illnesses are considered: the psychological and social consequences of deafness, the possible contribution of sensory deprivation phenomena, and the interference of hearing loss in attention, perception and communication. Finally, possible future lines of research are suggested.", "contents": "Deafness and psychiatric illness. Review of the literature concerning the relationship between deafness and psychiatric disorder reveals differences in the pattern of illness depending on the severity of deafness and the age of onset. In particular, the prevalence of schizophrenia in the prelingually deaf is similar to that found in the normal population, whereas the hard of hearing are over-represented among samples of patients suffering from paranoid psychoses in later life. Possible modes of action of long-standing hearing loss in the aetiology of paranoid illnesses are considered: the psychological and social consequences of deafness, the possible contribution of sensory deprivation phenomena, and the interference of hearing loss in attention, perception and communication. Finally, possible future lines of research are suggested.", "PMID": 963355}
{"id": "pubmed23n0032_5534", "title": "Platelet monoamine oxidase in schizophrenia and manic-depressive illness.", "content": "Monoamine oxidase (MAO) is an important enzyme in the catabolism of brain biogenic amines. Platelet MAO has been reported to be moderately reduced in manic-depressive patients and markedly reduced in schizophrenic patients. This enzyme's activity has been shown to be under a large degree of genetic control and has been proposed as a 'genetic marker' in schizophrenia. A transcultural replication of the finding of low platelet MAO in schizophrenia and manic-depressive illness was carried out at the Jerusalem Mental Health Centre. Manic-depressive patients were found to have higher platelet MAO activity than schizophrenic patients, as reported previously, but control individuals were as low as the schizophrenic patients. It is unlikely that platelet MAO activity is a transculturally-valid marker for schizophrenia.", "contents": "Platelet monoamine oxidase in schizophrenia and manic-depressive illness. Monoamine oxidase (MAO) is an important enzyme in the catabolism of brain biogenic amines. Platelet MAO has been reported to be moderately reduced in manic-depressive patients and markedly reduced in schizophrenic patients. This enzyme's activity has been shown to be under a large degree of genetic control and has been proposed as a 'genetic marker' in schizophrenia. A transcultural replication of the finding of low platelet MAO in schizophrenia and manic-depressive illness was carried out at the Jerusalem Mental Health Centre. Manic-depressive patients were found to have higher platelet MAO activity than schizophrenic patients, as reported previously, but control individuals were as low as the schizophrenic patients. It is unlikely that platelet MAO activity is a transculturally-valid marker for schizophrenia.", "PMID": 963356}
{"id": "pubmed23n0032_5535", "title": "Whole blood adenosine triphosphate in manic-depressive illness.", "content": "Whole blood cell ATP concentration was estimated in a group of manic and depressed patients and in a group of normal subjects. There were no significant differences between patient and control groups, nor in the patient groups were there any significant changes with recovery.", "contents": "Whole blood adenosine triphosphate in manic-depressive illness. Whole blood cell ATP concentration was estimated in a group of manic and depressed patients and in a group of normal subjects. There were no significant differences between patient and control groups, nor in the patient groups were there any significant changes with recovery.", "PMID": 963357}
{"id": "pubmed23n0032_5536", "title": "Thyroid dysfunction in female psychiatric patients.", "content": "Biochemical screening for thyroid dysfunction in 98 unselected female psychiatric admissions led to the diagnosis of hypothyroidism in three cases which would otherwise have passed unrecognized. It is suggested that female psychiatric patients over the age of 40 should routinely be screened for thyroid disorder.", "contents": "Thyroid dysfunction in female psychiatric patients. Biochemical screening for thyroid dysfunction in 98 unselected female psychiatric admissions led to the diagnosis of hypothyroidism in three cases which would otherwise have passed unrecognized. It is suggested that female psychiatric patients over the age of 40 should routinely be screened for thyroid disorder.", "PMID": 963358}
{"id": "pubmed23n0032_5537", "title": "Polygenic inheritance of epilepsy and febrile convulsions: analysis based on a computational model.", "content": "Two series of patients with epilepsy and with febrile convulsions were analysed on Slater's computational model for evidence on the mode of inheritance. The study demonstrated that in both series a polygenic inheritance may be more likely than an autosomal dominant or recessive inheritance.", "contents": "Polygenic inheritance of epilepsy and febrile convulsions: analysis based on a computational model. Two series of patients with epilepsy and with febrile convulsions were analysed on Slater's computational model for evidence on the mode of inheritance. The study demonstrated that in both series a polygenic inheritance may be more likely than an autosomal dominant or recessive inheritance.", "PMID": 963359}
{"id": "pubmed23n0032_5538", "title": "Physique, personality and mental illness in southern Chinese women.", "content": "A number of anthropometric measurements were made on Southern Chinese women. These were 317 schizophrenics, 44 with affective disorder, 47 neurotics and normal controls. All groups and sub-groups compared were matched for mean age and socio-economic status. It was found that the intercorrelations between body build, personality and type of mental illness were on the whole similar to those reported for Caucasians.", "contents": "Physique, personality and mental illness in southern Chinese women. A number of anthropometric measurements were made on Southern Chinese women. These were 317 schizophrenics, 44 with affective disorder, 47 neurotics and normal controls. All groups and sub-groups compared were matched for mean age and socio-economic status. It was found that the intercorrelations between body build, personality and type of mental illness were on the whole similar to those reported for Caucasians.", "PMID": 963360}
{"id": "pubmed23n0032_5539", "title": "Marriage and neurosis in a modern residential suburb: an application of the Ryle Marital Patterns Test.", "content": "The Ryle Marital Patterns Test measures the affection and domination aspects of marriage. Its original administration illustrated a partial association between poor marital adjustment (as measured by low affection scores and non-egalitarian domination scores) and neuroticism (as measured by CMI score). The present paper confirms these associations using a group of suburban women defined as neurotic by their GP. Related studies suggest similar patterns.", "contents": "Marriage and neurosis in a modern residential suburb: an application of the Ryle Marital Patterns Test. The Ryle Marital Patterns Test measures the affection and domination aspects of marriage. Its original administration illustrated a partial association between poor marital adjustment (as measured by low affection scores and non-egalitarian domination scores) and neuroticism (as measured by CMI score). The present paper confirms these associations using a group of suburban women defined as neurotic by their GP. Related studies suggest similar patterns.", "PMID": 963361}
{"id": "pubmed23n0032_5540", "title": "Help-seeking in depressed out-patients following maintenance therapy.", "content": "This paper reports on the clinical status, help-seeking and subsequent treatment experiences of 150 women one year after they had completed out-patient maintenance treatment by amitriptyline and/or psychotherapy for a depressive episode. While the majority of patients were asymptomatic at follow-up, a substantial minority had a return of acute symptoms and 2 per cent made minor suicide attempts during the year. Admission to hospital was rare. However, only 30 per cent of the patients did not seek any treatment during the year and the majority received some psychotropic medication. The findings support the long-term need for prompt access to treatment by patients who have recovered from an acute depression.", "contents": "Help-seeking in depressed out-patients following maintenance therapy. This paper reports on the clinical status, help-seeking and subsequent treatment experiences of 150 women one year after they had completed out-patient maintenance treatment by amitriptyline and/or psychotherapy for a depressive episode. While the majority of patients were asymptomatic at follow-up, a substantial minority had a return of acute symptoms and 2 per cent made minor suicide attempts during the year. Admission to hospital was rare. However, only 30 per cent of the patients did not seek any treatment during the year and the majority received some psychotropic medication. The findings support the long-term need for prompt access to treatment by patients who have recovered from an acute depression.", "PMID": 963362}
{"id": "pubmed23n0032_5541", "title": "Socio-cultural determinants of attempted suicide among West Indians in Birmingham: ethnic origin and immigrant status.", "content": "The author studied the significance of ethnic origin and immigrant status in attempted suicide. Epidemiological data (1969-72) indicated that self-poisoning among West Indian immigrants in Birmingham was less prevalent than among natives there but more prevalent than in the West Indies. The association of recent pregnancy with attempted suicide among young immigrant patients was noteworthy. Attempted suicide among immigrants was often relatively benign; few abused alcohol or drugs or made repeated attempts. The markedly lower male rate of attempted suicide, and its rarity in older persons, confirms previous findings.", "contents": "Socio-cultural determinants of attempted suicide among West Indians in Birmingham: ethnic origin and immigrant status. The author studied the significance of ethnic origin and immigrant status in attempted suicide. Epidemiological data (1969-72) indicated that self-poisoning among West Indian immigrants in Birmingham was less prevalent than among natives there but more prevalent than in the West Indies. The association of recent pregnancy with attempted suicide among young immigrant patients was noteworthy. Attempted suicide among immigrants was often relatively benign; few abused alcohol or drugs or made repeated attempts. The markedly lower male rate of attempted suicide, and its rarity in older persons, confirms previous findings.", "PMID": 963363}
{"id": "pubmed23n0032_5542", "title": "The aftermath of parental suicide for children.", "content": "Thirty-six children between the ages of 2 and 17 bereaved by the suicide of a parent were followed up after 5 to 17 years. The children's home life before bereavement had been abnormal because of mental illness, and family disruption and pre-suicide stresses were significantly related to present functioning. An incidence of psychological morbidity greater than that of a comparison group was observed. Some children appear to cope with the experience of parental suicide without serious consequences; for a few there was relief from an insupportable situation.", "contents": "The aftermath of parental suicide for children. Thirty-six children between the ages of 2 and 17 bereaved by the suicide of a parent were followed up after 5 to 17 years. The children's home life before bereavement had been abnormal because of mental illness, and family disruption and pre-suicide stresses were significantly related to present functioning. An incidence of psychological morbidity greater than that of a comparison group was observed. Some children appear to cope with the experience of parental suicide without serious consequences; for a few there was relief from an insupportable situation.", "PMID": 963364}
{"id": "pubmed23n0032_5543", "title": "The effect of microwave therapy upon muscle blood flow in man.", "content": "Muscle blood flow was measured using a radioactive tracer technique in five normal subjects. Flow values obtained during application of 2450 MHz microwave therapy were compared with resting values and in each case the treatment produced a significant increase; the mean values being 2.9 ml/100g/min at rest and 11.4 ml/100g/min during application of the microwave therapy. Comparison is made with previous studies using other therapeutic agents and it is concluded that the most significant effect on muscle blood flow is produced by microwave therapy.", "contents": "The effect of microwave therapy upon muscle blood flow in man. Muscle blood flow was measured using a radioactive tracer technique in five normal subjects. Flow values obtained during application of 2450 MHz microwave therapy were compared with resting values and in each case the treatment produced a significant increase; the mean values being 2.9 ml/100g/min at rest and 11.4 ml/100g/min during application of the microwave therapy. Comparison is made with previous studies using other therapeutic agents and it is concluded that the most significant effect on muscle blood flow is produced by microwave therapy.", "PMID": 963368}
{"id": "pubmed23n0032_5544", "title": "Some reactions to a dry-land training programme for dinghy sailors.", "content": "A dry-land winter training programme for dinghy-sailors is described. Individual elements include circuit training, specific exercises for muscle strength and endurance, and distance running. Ten international-class sailors followed a progressive regimen of this type for 14 weeks after completion of the 1973 season. Excess weight and skinfold thicknesses were reduced, while muscle strength, endurance and anaerobic capacity increased. Aerobic power remained substantially unchanged, although a smaller oxygen debt was incurred in reaching maximum effort. Team members were enthusiastic about the benefits gained from the training, commenting on their greater tolerance of the hiking position and all proposed to continue or to increase their efforts during subsequent winters. Racing results during 1974 were also an improvement on the 1973 record. However, it was difficult to link physiological gains to improvements in the relative rankings of individual competitors under either light or high wind conditions; the main factor changing relative standings seems to have been the additional year of competitive experiences in younger team members.", "contents": "Some reactions to a dry-land training programme for dinghy sailors. A dry-land winter training programme for dinghy-sailors is described. Individual elements include circuit training, specific exercises for muscle strength and endurance, and distance running. Ten international-class sailors followed a progressive regimen of this type for 14 weeks after completion of the 1973 season. Excess weight and skinfold thicknesses were reduced, while muscle strength, endurance and anaerobic capacity increased. Aerobic power remained substantially unchanged, although a smaller oxygen debt was incurred in reaching maximum effort. Team members were enthusiastic about the benefits gained from the training, commenting on their greater tolerance of the hiking position and all proposed to continue or to increase their efforts during subsequent winters. Racing results during 1974 were also an improvement on the 1973 record. However, it was difficult to link physiological gains to improvements in the relative rankings of individual competitors under either light or high wind conditions; the main factor changing relative standings seems to have been the additional year of competitive experiences in younger team members.", "PMID": 963372}
{"id": "pubmed23n0032_5545", "title": "British pilot study of exercise therapy. I. Middle-aged men.", "content": "The physiological and biochemical effects of a carefully graduated course of vigorous gymnasium training with two or three weekly exercise sessions lasting only 15 minutes have been studied in middle-aged London business men. Activity diaries and psychological questionnaires indicated that these men had a positive attitude to exercise which was probably greater than average. The gymnasium exercises caused a large oxygen debt and considerable rises in plasma catecholamines and lactate levels. A close correlation was found between the pulse rate during exercise and the Borg scale of perceived exertion, so that both could be used to ensure that short periods of exercise were sufficiently vigorous to produce a training effect, and protect against over-exertion. The acceptability of this particular exercise regime was shown by the low fall-out and injury rate. It is suggested that this exercise training programme possesses many features which are advantageous if increased physical activity is to be more widely used as a method of reducing some of the risk factors in coronary heart disease.", "contents": "British pilot study of exercise therapy. I. Middle-aged men. The physiological and biochemical effects of a carefully graduated course of vigorous gymnasium training with two or three weekly exercise sessions lasting only 15 minutes have been studied in middle-aged London business men. Activity diaries and psychological questionnaires indicated that these men had a positive attitude to exercise which was probably greater than average. The gymnasium exercises caused a large oxygen debt and considerable rises in plasma catecholamines and lactate levels. A close correlation was found between the pulse rate during exercise and the Borg scale of perceived exertion, so that both could be used to ensure that short periods of exercise were sufficiently vigorous to produce a training effect, and protect against over-exertion. The acceptability of this particular exercise regime was shown by the low fall-out and injury rate. It is suggested that this exercise training programme possesses many features which are advantageous if increased physical activity is to be more widely used as a method of reducing some of the risk factors in coronary heart disease.", "PMID": 963374}
{"id": "pubmed23n0032_5546", "title": "British pilot study of exercise therapy. II. Patients with cardiovascular disease.", "content": "Two groups of middle-aged men, one with and one without overt cardiovascular disease, were studied while they were taking part in a specially designed course of exercise therapy in a gymnasium. The \"patients\" group had at least two months pre-treatment to allow physical recovery and mental re-education before their initial very small test dose of exercise. Using short periods of progressive, mainly weight-loaded, isotonic exercises carefully regulated by control of pulse rate and avoidance of symptoms of over-exertion, both groups showed large increases in effort capacity and reductions in resting pulse rate, blood pressure and plasma lipid levels within two months. The safety of this particular form of exercise was shown in this high-risk population by the low drop-out rate and the absence of cardiovascular accidents in the gymnasium over a ten year period. It is suggested that, given suitable training of the staff and using the safeguards described, the presence of doctors and a cardiac resuscitation team is unnecessary in a gymnasium specializing in cardiac rehabilitation. This makes it possible for rehabilitation and physiotherapy departments throughout the country to carry out this effective and positive form of exercise therapy.", "contents": "British pilot study of exercise therapy. II. Patients with cardiovascular disease. Two groups of middle-aged men, one with and one without overt cardiovascular disease, were studied while they were taking part in a specially designed course of exercise therapy in a gymnasium. The \"patients\" group had at least two months pre-treatment to allow physical recovery and mental re-education before their initial very small test dose of exercise. Using short periods of progressive, mainly weight-loaded, isotonic exercises carefully regulated by control of pulse rate and avoidance of symptoms of over-exertion, both groups showed large increases in effort capacity and reductions in resting pulse rate, blood pressure and plasma lipid levels within two months. The safety of this particular form of exercise was shown in this high-risk population by the low drop-out rate and the absence of cardiovascular accidents in the gymnasium over a ten year period. It is suggested that, given suitable training of the staff and using the safeguards described, the presence of doctors and a cardiac resuscitation team is unnecessary in a gymnasium specializing in cardiac rehabilitation. This makes it possible for rehabilitation and physiotherapy departments throughout the country to carry out this effective and positive form of exercise therapy.", "PMID": 963375}
{"id": "pubmed23n0032_5547", "title": "Maximal exercise studies in Scottish athletes.", "content": "We have studied maximal oxygen uptake, maximal heart rate, maximal exercise ventilation and the ventilatory response to exercise in 24 male and 14 female Scottish athletes. The values for maximal oxygen uptake are similar to those reported in other international studies. In eight athletes repeat measurements of maximal oxygen uptake 7-11 months after the initial study showed no change from the initial values. The values for the ventilatory response to exercise in our athletes were normal. The relationship is discussed between maximal oxygen uptake and ventilatory responses to exercise, hypoxia and hypercapnia and it is suggested that these may be related to athletic event.", "contents": "Maximal exercise studies in Scottish athletes. We have studied maximal oxygen uptake, maximal heart rate, maximal exercise ventilation and the ventilatory response to exercise in 24 male and 14 female Scottish athletes. The values for maximal oxygen uptake are similar to those reported in other international studies. In eight athletes repeat measurements of maximal oxygen uptake 7-11 months after the initial study showed no change from the initial values. The values for the ventilatory response to exercise in our athletes were normal. The relationship is discussed between maximal oxygen uptake and ventilatory responses to exercise, hypoxia and hypercapnia and it is suggested that these may be related to athletic event.", "PMID": 963376}
{"id": "pubmed23n0032_5548", "title": "The reproducibility of submaximal work heart rates-an interim report.", "content": "Work heart rates were taken from four subjects performing standardized bouts of work on the Monark Ergometer. Whilst a linear relationship was observed between heart rate and work intensity on any one day, it is evident that day to day variations in the position (intercept) of these lines exists. The slopes are virtually identical for all subjects and for different days, however the position of the lines differs widely both between and within subjects. The best linear regression equation developed for the prediction of heart rate after 180 seconds of work from pre-exercise heart rate and work intensity was found to have 95% confidence limits of +/- 12.7 beats. Heart rates are reproducible 95% of the time to within +/- 12.2 beats when pre-exercise heart rate is ignored.", "contents": "The reproducibility of submaximal work heart rates-an interim report. Work heart rates were taken from four subjects performing standardized bouts of work on the Monark Ergometer. Whilst a linear relationship was observed between heart rate and work intensity on any one day, it is evident that day to day variations in the position (intercept) of these lines exists. The slopes are virtually identical for all subjects and for different days, however the position of the lines differs widely both between and within subjects. The best linear regression equation developed for the prediction of heart rate after 180 seconds of work from pre-exercise heart rate and work intensity was found to have 95% confidence limits of +/- 12.7 beats. Heart rates are reproducible 95% of the time to within +/- 12.2 beats when pre-exercise heart rate is ignored.", "PMID": 963377}
{"id": "pubmed23n0032_5549", "title": "A simple new formula for relating changes in renal length to changes in renal volume.", "content": "A simple new formula for relating changes in renal length to changes in renal volume is derived. It is not assumed that the kidney is any given shape, but it is assumed that a kidney does not change its shape when it enlarges. It is shown that the fractional change in renal volume equals three times the fractional change in renal length plus three times the fractional change in renal length squared. Examples of applications of the formula using figures for changes in renal length, published elsewhere in the literature, are given for renal hypertrophy in both childhood and adult life and for volume changes during excretion urography.", "contents": "A simple new formula for relating changes in renal length to changes in renal volume. A simple new formula for relating changes in renal length to changes in renal volume is derived. It is not assumed that the kidney is any given shape, but it is assumed that a kidney does not change its shape when it enlarges. It is shown that the fractional change in renal volume equals three times the fractional change in renal length plus three times the fractional change in renal length squared. Examples of applications of the formula using figures for changes in renal length, published elsewhere in the literature, are given for renal hypertrophy in both childhood and adult life and for volume changes during excretion urography.", "PMID": 963383}
{"id": "pubmed23n0032_5550", "title": "Thallium 201 chloride uptake in tumours, a possible complication in heart scintigraphy.", "content": "An abnormal uptake pattern of 201Tl thallous chloride observed during myocardial scintigraphy of a patient with known thoracic tumour localization suggested the possibility that thallous chloride may also accumulate in tumours. This was confirmed by observing 201thallous chloride accumulation in rhabdomyosarcoma implants in rats. Thallium 201 accumulation in tumour cells should be borne in mind as a possible complicating factor when using this reagent for the evaluation of cardiac conditions in oncological patients.", "contents": "Thallium 201 chloride uptake in tumours, a possible complication in heart scintigraphy. An abnormal uptake pattern of 201Tl thallous chloride observed during myocardial scintigraphy of a patient with known thoracic tumour localization suggested the possibility that thallous chloride may also accumulate in tumours. This was confirmed by observing 201thallous chloride accumulation in rhabdomyosarcoma implants in rats. Thallium 201 accumulation in tumour cells should be borne in mind as a possible complicating factor when using this reagent for the evaluation of cardiac conditions in oncological patients.", "PMID": 963384}
{"id": "pubmed23n0032_5551", "title": "Pharmaco-cholangiography with anticholinergic drugs in the dog.", "content": "The effect of atropine and pipenzolate bromide (PB) in different dosage levels was investigated in intravenous cholangiography with 0.6 ml/kg iodipamide infused over 30 minutes in six cholecystectomized dogs (20-36 kg) equipped with Thomas cannulas through which the common bile duct could be cannulated. Doses of 1 mg atropine and 20 mg PB, half the dose given intravenously just prior to the contrast agent and the other half with the iodipamide infusion, had the greatest effect in decreasing the bile flow (atropine-24% at 60, PB-23% at 30 minutes) and increasing the bile iodine concentration (atropine + 16%, PB + 14%). The biliary iodipamide excretion rate was not affected.", "contents": "Pharmaco-cholangiography with anticholinergic drugs in the dog. The effect of atropine and pipenzolate bromide (PB) in different dosage levels was investigated in intravenous cholangiography with 0.6 ml/kg iodipamide infused over 30 minutes in six cholecystectomized dogs (20-36 kg) equipped with Thomas cannulas through which the common bile duct could be cannulated. Doses of 1 mg atropine and 20 mg PB, half the dose given intravenously just prior to the contrast agent and the other half with the iodipamide infusion, had the greatest effect in decreasing the bile flow (atropine-24% at 60, PB-23% at 30 minutes) and increasing the bile iodine concentration (atropine + 16%, PB + 14%). The biliary iodipamide excretion rate was not affected.", "PMID": 963385}
{"id": "pubmed23n0032_5552", "title": "Liver volume determination by ultrasound: a feasibility study.", "content": "The feasibility and precision of an ultrasound method for determining the volume of the liver have been assessed using a Nuclear Enterprises B-scan machine. A relation between estimated liver volume and body weight has been established, showing that liver volume can be determined with a precision of +/- 195 cm3 (P less than or equal 0-05). With the standard non-grey-scale imaging technique employed there is a degree of uncertainty in deciding the liver boundaries, and this constitutes the major source of error.", "contents": "Liver volume determination by ultrasound: a feasibility study. The feasibility and precision of an ultrasound method for determining the volume of the liver have been assessed using a Nuclear Enterprises B-scan machine. A relation between estimated liver volume and body weight has been established, showing that liver volume can be determined with a precision of +/- 195 cm3 (P less than or equal 0-05). With the standard non-grey-scale imaging technique employed there is a degree of uncertainty in deciding the liver boundaries, and this constitutes the major source of error.", "PMID": 963386}
{"id": "pubmed23n0032_5553", "title": "The analysis of radioisotope brain scans by comparison with normal patterns of uptake.", "content": "A previously described method for the computer analysis of radioisotope brain scans has been further developed. A patient scan is matched with a series of \"normal\" scans to find the closest fit and the \"normal\" scan is then subtracted from the patient scan. The results are displayed in terms of the standard deviation from the normal. In a clinical trial the computer assessments were found to be less accurate than those of two groups of observers. One reason for these disappointing results is related to the simple criteria used to choose and fit the best \"normal\" to a particular patient scan. A second reason is the optimization of the scanner/photoscan combination to 99Tcm pertechnetate as a brain scanning agent which results in patient-to-patient variation becoming more important. This reduces the potential of a simple numerical analysis to improve results.", "contents": "The analysis of radioisotope brain scans by comparison with normal patterns of uptake. A previously described method for the computer analysis of radioisotope brain scans has been further developed. A patient scan is matched with a series of \"normal\" scans to find the closest fit and the \"normal\" scan is then subtracted from the patient scan. The results are displayed in terms of the standard deviation from the normal. In a clinical trial the computer assessments were found to be less accurate than those of two groups of observers. One reason for these disappointing results is related to the simple criteria used to choose and fit the best \"normal\" to a particular patient scan. A second reason is the optimization of the scanner/photoscan combination to 99Tcm pertechnetate as a brain scanning agent which results in patient-to-patient variation becoming more important. This reduces the potential of a simple numerical analysis to improve results.", "PMID": 963387}
{"id": "pubmed23n0032_5554", "title": "Vaginal ureterolithotomy.", "content": "The vaginal approach to the low ureteric calculus may provide an easy, rapid and safe solution to a difficult surgical problem. It is important to select the case with care and, provided basic surgical principles are followed, the complications should be minimal. 3 recent successful cases are described and a further 2 in which the technique failed due to proximal displacement of the stone. The importance of adequately immobilising the calculus prior to ureterotomy is emphasised.", "contents": "Vaginal ureterolithotomy. The vaginal approach to the low ureteric calculus may provide an easy, rapid and safe solution to a difficult surgical problem. It is important to select the case with care and, provided basic surgical principles are followed, the complications should be minimal. 3 recent successful cases are described and a further 2 in which the technique failed due to proximal displacement of the stone. The importance of adequately immobilising the calculus prior to ureterotomy is emphasised.", "PMID": 963395}
{"id": "pubmed23n0032_5555", "title": "The use of bromocriptine in the treatment of the unstable bladder.", "content": "Initial experience with the use of bromocriptine in 24 patients with troublesome micturition symptoms associated with an unstable bladder is described. 14 patients responded symptomatically to treatment with bromocriptine and these results were confirmed by a double blind trial in this group. 11 of these patients had previously failed to benefit from treatment either with drugs or vesical distension. It is concluded that bromocriptine has a part to play in the treatment of some cases of primary detrusor instability.", "contents": "The use of bromocriptine in the treatment of the unstable bladder. Initial experience with the use of bromocriptine in 24 patients with troublesome micturition symptoms associated with an unstable bladder is described. 14 patients responded symptomatically to treatment with bromocriptine and these results were confirmed by a double blind trial in this group. 11 of these patients had previously failed to benefit from treatment either with drugs or vesical distension. It is concluded that bromocriptine has a part to play in the treatment of some cases of primary detrusor instability.", "PMID": 963397}
{"id": "pubmed23n0032_5556", "title": "Bladder and urethral innervation in multiple sclerosis.", "content": "Bladder and urethral innervation was studied in 52 patients with multiple sclerosis using a signal tracing technique (evoked reflex latency measurement).  The majority of the patients showed prolonged signal transit times indicative of demyelinating plaques localised to the lumbosacral spinal cord.  Futhermore, impairment of the corticospinal innervation of the pudendal nucleus was found in 29 patients indicating lesions of the pyramidal tract.", "contents": "Bladder and urethral innervation in multiple sclerosis. Bladder and urethral innervation was studied in 52 patients with multiple sclerosis using a signal tracing technique (evoked reflex latency measurement).  The majority of the patients showed prolonged signal transit times indicative of demyelinating plaques localised to the lumbosacral spinal cord.  Futhermore, impairment of the corticospinal innervation of the pudendal nucleus was found in 29 patients indicating lesions of the pyramidal tract.", "PMID": 963398}
{"id": "pubmed23n0032_5557", "title": "Chronic bacterial prostatitis: new therapeutic aspects.", "content": "6 cases with chronic bacterial prostatitis were reviewed. The effort and patience required to diagnose this condition accurately by means of differential urine and prostatic secretion cultures is extremely worthwhile. Cotrimoxazole, which has been suggested as a most effective antibacterial drug in chronic bacterial prostatitis, cured only 3 out of our 6 patients and then only after continuous treatment for 4 months. 2 of the remaining patients were cured by a 10 to 14 days' course of kanamycin and the third was probably cured by streptomycin. On theoretical grounds neither of these lipid insoluble drugs should be capable of penetrating the prostatic epithelium. The implications of these findings are discussed.", "contents": "Chronic bacterial prostatitis: new therapeutic aspects. 6 cases with chronic bacterial prostatitis were reviewed. The effort and patience required to diagnose this condition accurately by means of differential urine and prostatic secretion cultures is extremely worthwhile. Cotrimoxazole, which has been suggested as a most effective antibacterial drug in chronic bacterial prostatitis, cured only 3 out of our 6 patients and then only after continuous treatment for 4 months. 2 of the remaining patients were cured by a 10 to 14 days' course of kanamycin and the third was probably cured by streptomycin. On theoretical grounds neither of these lipid insoluble drugs should be capable of penetrating the prostatic epithelium. The implications of these findings are discussed.", "PMID": 963400}
{"id": "pubmed23n0032_5558", "title": "Random bone marrow biopsy in the staging of carcinoma of the prostate.", "content": "Random bone marrow biopsies were examined in 28 patients with proven carcinoma of the prostate. In 6 cases (28-5%) bony metastases were demonstrated and in 2 of these X-ray skeletal surveys were negative. It is suggested that bone marrow biopsy should be done in the staging of carcinoma of the prostate when other investigations are negative, especially when an occult primary is suspected, when radical treatment is contemplated and in clinical trials.", "contents": "Random bone marrow biopsy in the staging of carcinoma of the prostate. Random bone marrow biopsies were examined in 28 patients with proven carcinoma of the prostate. In 6 cases (28-5%) bony metastases were demonstrated and in 2 of these X-ray skeletal surveys were negative. It is suggested that bone marrow biopsy should be done in the staging of carcinoma of the prostate when other investigations are negative, especially when an occult primary is suspected, when radical treatment is contemplated and in clinical trials.", "PMID": 963403}
{"id": "pubmed23n0032_5559", "title": "Vasoplasty: a new technique.", "content": "A technique by which plain catgut is used as an internal splint in vasoplasty is described. 28 cases have been carried out, of which only 4 have failed.", "contents": "Vasoplasty: a new technique. A technique by which plain catgut is used as an internal splint in vasoplasty is described. 28 cases have been carried out, of which only 4 have failed.", "PMID": 963406}
{"id": "pubmed23n0032_5560", "title": "Comparison of sterile bag, clean catch and suprapubic aspiration in the diagnosis of urinary infection in early childhood.", "content": "Bag, clean catch and suprapubic specimens of urine were taken on the same day in a group of 30 unwell infants and young children. On microbiological grounds suprapubic aspiration was far superior to clean catch collection and clean catch collection superior to bag collection in the diagnosis of urinary tract infection.", "contents": "Comparison of sterile bag, clean catch and suprapubic aspiration in the diagnosis of urinary infection in early childhood. Bag, clean catch and suprapubic specimens of urine were taken on the same day in a group of 30 unwell infants and young children. On microbiological grounds suprapubic aspiration was far superior to clean catch collection and clean catch collection superior to bag collection in the diagnosis of urinary tract infection.", "PMID": 963408}
{"id": "pubmed23n0032_5561", "title": "Comparison of hydrophilic polymer-coated latex, uncoated latex and PVC indwelling balloon catheters in the prevention of urinary infection.", "content": "Latex, hydrophilic polymer-coated latex and PVC balloon indwelling urethral catheters were compared in respect of the urinary tract infections arising in association with their use in male patients. The polymer (Hydron) coating conferred no benefit over uncoated latex which in turn was indistinguishable from PVC. No significant differences in the spectra of infecting organismns were observed between the 3 catheter types.", "contents": "Comparison of hydrophilic polymer-coated latex, uncoated latex and PVC indwelling balloon catheters in the prevention of urinary infection. Latex, hydrophilic polymer-coated latex and PVC balloon indwelling urethral catheters were compared in respect of the urinary tract infections arising in association with their use in male patients. The polymer (Hydron) coating conferred no benefit over uncoated latex which in turn was indistinguishable from PVC. No significant differences in the spectra of infecting organismns were observed between the 3 catheter types.", "PMID": 963410}
{"id": "pubmed23n0032_5562", "title": "Colo-anal sleeve anastomosis in the treatment of diffuse cavernous haemangioma involving the rectum.", "content": "An operative technique, involving a colo-anal sleeve anastomosis, is described for the treatment of cavernous haemangioma of the rectum. All the patients with this condition treated since 1930 at St Mark's Hospital are reviewed, and the presentation and results of treatment in the last 5 patients who have had a resection and colo-anal sleeve anastomosis are discussed.", "contents": "Colo-anal sleeve anastomosis in the treatment of diffuse cavernous haemangioma involving the rectum. An operative technique, involving a colo-anal sleeve anastomosis, is described for the treatment of cavernous haemangioma of the rectum. All the patients with this condition treated since 1930 at St Mark's Hospital are reviewed, and the presentation and results of treatment in the last 5 patients who have had a resection and colo-anal sleeve anastomosis are discussed.", "PMID": 963412}
{"id": "pubmed23n0032_5563", "title": "The effect of an infusion of low molecular weight dextran on peripheral resistance in patients with arteriosclerosis.", "content": "Low molecular weight dextran is frequently given to improve the flow of blood to the periphery, but its mode of action is uncertain. We measured the effect of an infusion of 500 ml of Dextran 40 on the peripheral resistance in 12 patients undergoing reconstructive surgery for atherosclerotic occlusion of the superficial femoral artery. After the infusion, values of packed cell volume and blood viscosity indicated haemodilution. There was a consistent increase in mean flow and a fall in peripheral resistance. There was no correlation between change in viscosity and change in peripheral resistance, but there was a good correlation between the percentage change in resistance and the initial resistance. This suggests that the increase in flow of blood was due to reflex vasodilatation rather than to any reduction in viscosity.", "contents": "The effect of an infusion of low molecular weight dextran on peripheral resistance in patients with arteriosclerosis. Low molecular weight dextran is frequently given to improve the flow of blood to the periphery, but its mode of action is uncertain. We measured the effect of an infusion of 500 ml of Dextran 40 on the peripheral resistance in 12 patients undergoing reconstructive surgery for atherosclerotic occlusion of the superficial femoral artery. After the infusion, values of packed cell volume and blood viscosity indicated haemodilution. There was a consistent increase in mean flow and a fall in peripheral resistance. There was no correlation between change in viscosity and change in peripheral resistance, but there was a good correlation between the percentage change in resistance and the initial resistance. This suggests that the increase in flow of blood was due to reflex vasodilatation rather than to any reduction in viscosity.", "PMID": 963413}
{"id": "pubmed23n0032_5564", "title": "Plasma fibrinolysis and postoperative deep vein thrombosis.", "content": "In 23 high risk patients the change in plasminogen activator activity in response to surgical operation was studied by euglobulin lysis time (ELT) and fibrin plate lysis before, during and for up to 6 days following a major surgical procedure. Fibrin degradation products (FDP) were also measured. The aim was to relate any changes to postoperative deep vein thrombosis (DVT) as diagnosed by the 125I fibrinogen test. Peroperative increase and postoperative inhibition of fibrinolytic activity were seen in all the patients. Changes in fibrinolytic activity as measured by the ELT and during the first 24 hours by the fibrin plate technique were similar. This suggests that during this period the response was independent of plasma fibrinogen changes. There was no significant difference in these parameters between patients who developed DVT and those who did not. The relationship between venous thrombo-embolism and elevation of serum FDP was confirmed.", "contents": "Plasma fibrinolysis and postoperative deep vein thrombosis. In 23 high risk patients the change in plasminogen activator activity in response to surgical operation was studied by euglobulin lysis time (ELT) and fibrin plate lysis before, during and for up to 6 days following a major surgical procedure. Fibrin degradation products (FDP) were also measured. The aim was to relate any changes to postoperative deep vein thrombosis (DVT) as diagnosed by the 125I fibrinogen test. Peroperative increase and postoperative inhibition of fibrinolytic activity were seen in all the patients. Changes in fibrinolytic activity as measured by the ELT and during the first 24 hours by the fibrin plate technique were similar. This suggests that during this period the response was independent of plasma fibrinogen changes. There was no significant difference in these parameters between patients who developed DVT and those who did not. The relationship between venous thrombo-embolism and elevation of serum FDP was confirmed.", "PMID": 963414}
{"id": "pubmed23n0032_5565", "title": "Benign oesophageal stricture following oral potassium chloride therapy.", "content": "A patient is described who developed a benign oesophageal stricture following cardiac surgery. A brief review of the literature is given and the possible dangers of oral potassium cholride therapy in patients with dysphagia are highlighted.", "contents": "Benign oesophageal stricture following oral potassium chloride therapy. A patient is described who developed a benign oesophageal stricture following cardiac surgery. A brief review of the literature is given and the possible dangers of oral potassium cholride therapy in patients with dysphagia are highlighted.", "PMID": 963415}
{"id": "pubmed23n0032_5566", "title": "Perforated peptic ulcer: a comparison of long term results following partial gastric resection or simple closure.", "content": "The results of simple closure were compared with those of partial resection in the treatment of perforated peptic ulcer. The investigation was carried out 7-13 years after the primary operation on 126 patients who had been allotted to one of the two treatment methods on the basis of an almost randomized schedule. Better late results were obtained with partial resection than with simple closure in patients in the age range 50-59 years at operation, with short duration of perforation and with a long history of symptoms before perforation. None of the patients treated with partial resection later underwent surgical treatment for recurrence of symptoms. In the simple closure group 27.3 per cent needed further surgery at 3 months to 10 years after perforation.", "contents": "Perforated peptic ulcer: a comparison of long term results following partial gastric resection or simple closure. The results of simple closure were compared with those of partial resection in the treatment of perforated peptic ulcer. The investigation was carried out 7-13 years after the primary operation on 126 patients who had been allotted to one of the two treatment methods on the basis of an almost randomized schedule. Better late results were obtained with partial resection than with simple closure in patients in the age range 50-59 years at operation, with short duration of perforation and with a long history of symptoms before perforation. None of the patients treated with partial resection later underwent surgical treatment for recurrence of symptoms. In the simple closure group 27.3 per cent needed further surgery at 3 months to 10 years after perforation.", "PMID": 963416}
{"id": "pubmed23n0032_5567", "title": "Functional results of different reconstructive procedures after total gastrectomy.", "content": "In 181 patients six reconstructive procedures were performed after total gastrectomy: Roux-en-Y (95), Henley (42), omega (21), Tomoda (16, Rosanov (4) and neogastrium (3). The functional results 2-4 months after operation were studied and compared by medical observers independently of the surgeons who had selected and performed the reconstructive procedures. Analysis of the functional results, and consideration of the degree to which the functional disorders associated with the various reconstructive procedures lend themselves to treatment, suggest that the Henley reconstructive procedure is the best, with the Roux-en-Y as a good second-best.", "contents": "Functional results of different reconstructive procedures after total gastrectomy. In 181 patients six reconstructive procedures were performed after total gastrectomy: Roux-en-Y (95), Henley (42), omega (21), Tomoda (16, Rosanov (4) and neogastrium (3). The functional results 2-4 months after operation were studied and compared by medical observers independently of the surgeons who had selected and performed the reconstructive procedures. Analysis of the functional results, and consideration of the degree to which the functional disorders associated with the various reconstructive procedures lend themselves to treatment, suggest that the Henley reconstructive procedure is the best, with the Roux-en-Y as a good second-best.", "PMID": 963417}
{"id": "pubmed23n0032_5568", "title": "Duodenoscopic papillotomy and gallstone removal.", "content": "Using an experimental insulated duodenoscope and a diathermy wire, papillotomy has been performed on 10 patients under diazepam sedation. Seven patients had common bile duct stones (6 following cholecystectomy) and 3 had papillary stenosis. Nine of the patients had severe medical or surgical contraindications to further operative treatment. Papillotomy was successful in all patients, with no significant bleeding or other complications. Repeat endoscopy 6-14 days after papillotomy demonstrated a healed biliary orifice of 7-10 mm diameter. Stones had already passed in 3 of the 7 patients; in 2 others, stones were extracted using balloon catheters or Dormia type baskets. Two larger stones could not be removed; in one of these patients the stone and basket became impacted at the papilla and had to be removed surgically 2 days later. Endoscopic papillotomy is an acceptable alternative to surgery in high risk patients, particularly in the treatment of retained common bile duct stones. If long term results prove satisfactory, the technique will have wider application.", "contents": "Duodenoscopic papillotomy and gallstone removal. Using an experimental insulated duodenoscope and a diathermy wire, papillotomy has been performed on 10 patients under diazepam sedation. Seven patients had common bile duct stones (6 following cholecystectomy) and 3 had papillary stenosis. Nine of the patients had severe medical or surgical contraindications to further operative treatment. Papillotomy was successful in all patients, with no significant bleeding or other complications. Repeat endoscopy 6-14 days after papillotomy demonstrated a healed biliary orifice of 7-10 mm diameter. Stones had already passed in 3 of the 7 patients; in 2 others, stones were extracted using balloon catheters or Dormia type baskets. Two larger stones could not be removed; in one of these patients the stone and basket became impacted at the papilla and had to be removed surgically 2 days later. Endoscopic papillotomy is an acceptable alternative to surgery in high risk patients, particularly in the treatment of retained common bile duct stones. If long term results prove satisfactory, the technique will have wider application.", "PMID": 963418}
{"id": "pubmed23n0032_5569", "title": "An acute abdomen: spontaneous rupture of liver during pregnancy.", "content": "Spontaneous rupture of liver during pregnancy is presented as an example of an acute abdomen admitted to a casualty department of a general hospital. The literature is reviewed and emphasis is placed on clinical awareness and earlier diagnosis as a means to reduce the high mortality.", "contents": "An acute abdomen: spontaneous rupture of liver during pregnancy. Spontaneous rupture of liver during pregnancy is presented as an example of an acute abdomen admitted to a casualty department of a general hospital. The literature is reviewed and emphasis is placed on clinical awareness and earlier diagnosis as a means to reduce the high mortality.", "PMID": 963419}
{"id": "pubmed23n0032_5570", "title": "The microflora of the obstructed bowel.", "content": "A study of the alterations of intestinal microflora that occur in acute small and large bowel obstruction is presented. The results are discussed in relation to the duration of obstruction, the site of obstruction, the recovery following relief of obstruction and the septic complications which profoundly affect the prognosis of these common surgical emergencies.", "contents": "The microflora of the obstructed bowel. A study of the alterations of intestinal microflora that occur in acute small and large bowel obstruction is presented. The results are discussed in relation to the duration of obstruction, the site of obstruction, the recovery following relief of obstruction and the septic complications which profoundly affect the prognosis of these common surgical emergencies.", "PMID": 963420}
{"id": "pubmed23n0032_5571", "title": "Malignant cold thyroid nodules in hyperthyroidism.", "content": "In 65 hyperthyroid patients with a cold nodule a carcinoma was found at operation in 14 cases, i.e. a malignancy incidence of 21-5 per cent. In 859 euthyroid cases with a cold thyroid nodule 104 malignant lesions (12-1 per cent) were found. The difference is statistically significant. Surgery in cases with a cold thyroid nodule is strongly indicated if this occurs in a hyperthyroid gland.", "contents": "Malignant cold thyroid nodules in hyperthyroidism. In 65 hyperthyroid patients with a cold nodule a carcinoma was found at operation in 14 cases, i.e. a malignancy incidence of 21-5 per cent. In 859 euthyroid cases with a cold thyroid nodule 104 malignant lesions (12-1 per cent) were found. The difference is statistically significant. Surgery in cases with a cold thyroid nodule is strongly indicated if this occurs in a hyperthyroid gland.", "PMID": 963421}
{"id": "pubmed23n0032_5572", "title": "Active observation in management of acute abdominal pain in childhood.", "content": "Out of 363 children admitted to a surgical unit with acute abdominal pain only 126 (35%) had an operation. Of these, 20 first underwent a period of \"active observation.\" This procedure, used to help reach a diagnosis or decision in doubtful cases, is safe and useful and may be applied at home or in hospital.", "contents": "Active observation in management of acute abdominal pain in childhood. Out of 363 children admitted to a surgical unit with acute abdominal pain only 126 (35%) had an operation. Of these, 20 first underwent a period of \"active observation.\" This procedure, used to help reach a diagnosis or decision in doubtful cases, is safe and useful and may be applied at home or in hospital.", "PMID": 963429}
{"id": "pubmed23n0032_5573", "title": "Prevalence of primary and secondary hypertension: studies in a random population sample.", "content": "The prevalence of primary and secondary hypertension was determined in a random sample of 7455 Swedish men aged 47 to 54 years. Three hundred and sizty-one men were undergoing treatment for hypertension. Seven hundred and ninety-eight men who had blood pressures above 175/115 mm Hg at preliminary screening were recalled for further blood pressure measurements. Those on treatment and all the untreated men whose blood pressures were still over 175/115 mm Hg then underwent extensive investigation for secondary hypertension. Renal parenchymal hypertension was found in 25 (3-6%) patients, renovascular hypertension in four (0-6%), and other forms of secondary hypertension in 11 (1-6%). The investigation led to surgical treatment in only two cases (0-3%). The low prevalence of secondary hypertension, especially surgically curable forms of hypertension, makes routine screening for these cases unnecessary, at least when patients with hypertension have been found at screening. These data must be taken into account in planning community control programmes in hypertension.", "contents": "Prevalence of primary and secondary hypertension: studies in a random population sample. The prevalence of primary and secondary hypertension was determined in a random sample of 7455 Swedish men aged 47 to 54 years. Three hundred and sizty-one men were undergoing treatment for hypertension. Seven hundred and ninety-eight men who had blood pressures above 175/115 mm Hg at preliminary screening were recalled for further blood pressure measurements. Those on treatment and all the untreated men whose blood pressures were still over 175/115 mm Hg then underwent extensive investigation for secondary hypertension. Renal parenchymal hypertension was found in 25 (3-6%) patients, renovascular hypertension in four (0-6%), and other forms of secondary hypertension in 11 (1-6%). The investigation led to surgical treatment in only two cases (0-3%). The low prevalence of secondary hypertension, especially surgically curable forms of hypertension, makes routine screening for these cases unnecessary, at least when patients with hypertension have been found at screening. These data must be taken into account in planning community control programmes in hypertension.", "PMID": 963430}
{"id": "pubmed23n0032_5574", "title": "Hepatitis B virus infection in dental surgical practice.", "content": "Sixty-one dental surgeons at King's College Hospital were interviewed to establish the incidence of attacks of viral hepatitis and to relate this to environmental risk factors. Six (10%) had a history of hepatitis, in one case due to infection with the hepatitis B virus. Screening blood for HBsAg by radioimmunoassay showed no carriers of the antigen, but transient antigenaemia was observed in one dentist. Antibody to HBsAg, tested by radioimmunoassay, was detected in four dentists (7%), only one of whom had had clinical hepatitis. Dental surgeons may be more at risk from infection with the hepatitis B virus than the general population, although this should be minimised in hospital practice, where the most infected patients will already have been identified and appropriate precautions can be taken. The risk of transmission from an antigen-positive dentist to his patients is probably much smaller, and there is no evidence to restrict his clinical activities.", "contents": "Hepatitis B virus infection in dental surgical practice. Sixty-one dental surgeons at King's College Hospital were interviewed to establish the incidence of attacks of viral hepatitis and to relate this to environmental risk factors. Six (10%) had a history of hepatitis, in one case due to infection with the hepatitis B virus. Screening blood for HBsAg by radioimmunoassay showed no carriers of the antigen, but transient antigenaemia was observed in one dentist. Antibody to HBsAg, tested by radioimmunoassay, was detected in four dentists (7%), only one of whom had had clinical hepatitis. Dental surgeons may be more at risk from infection with the hepatitis B virus than the general population, although this should be minimised in hospital practice, where the most infected patients will already have been identified and appropriate precautions can be taken. The risk of transmission from an antigen-positive dentist to his patients is probably much smaller, and there is no evidence to restrict his clinical activities.", "PMID": 963431}
{"id": "pubmed23n0032_5575", "title": "Career problems of women doctors.", "content": "Information was received from 61 women doctors who were having difficulty continuing with medical careers. Two main problems were disclosed. Firstly, despite the special arrangements made for women doctors, it is difficult to obtain postgraduate training. The provision of supernumerary posts does not seem to offer a satisfactory solution. Secondly, doctors who have completed postgraduate training but cannot yet return to full-time work are unable to obtain posts at an appropriate level. Both of these problems stem primarily from the need for part-time work by the mothers of young children. Most of the doctors wish to return to full-time or nearly full-time work when family responsibilities are fewer. In view of the increasing proportion of women doctors it seems important that large numbers are not unnecessarily lost from professional work. Some possible approaches to solving the problems are suggested.", "contents": "Career problems of women doctors. Information was received from 61 women doctors who were having difficulty continuing with medical careers. Two main problems were disclosed. Firstly, despite the special arrangements made for women doctors, it is difficult to obtain postgraduate training. The provision of supernumerary posts does not seem to offer a satisfactory solution. Secondly, doctors who have completed postgraduate training but cannot yet return to full-time work are unable to obtain posts at an appropriate level. Both of these problems stem primarily from the need for part-time work by the mothers of young children. Most of the doctors wish to return to full-time or nearly full-time work when family responsibilities are fewer. In view of the increasing proportion of women doctors it seems important that large numbers are not unnecessarily lost from professional work. Some possible approaches to solving the problems are suggested.", "PMID": 963438}
{"id": "pubmed23n0032_5576", "title": "Use of creatine kinase for detecting severe X-linked muscular dystrophy carriers.", "content": "Women thought to be at risk of being carriers of Duchenne muscular dystrophy were given \"odds\" against their having an affected child. These were calcuated from a combination of the genetic risk from the family history and an estimation of the biochemical risk from measuring the serum creatine kinase concentration. The women were told the actual risk estimate and it was put into perspective for them as a high, medium, or low risk. Of 25 women at high risk six have had children, all girls; the two in the medium-risk group have had no children; and the 46 women at low risk have had 19 boys and 25 girls. None of the boys has the disease. With detailed counselling most potential carriers of this disease reach decisions in child bearing that are in line with their degree of risk.", "contents": "Use of creatine kinase for detecting severe X-linked muscular dystrophy carriers. Women thought to be at risk of being carriers of Duchenne muscular dystrophy were given \"odds\" against their having an affected child. These were calcuated from a combination of the genetic risk from the family history and an estimation of the biochemical risk from measuring the serum creatine kinase concentration. The women were told the actual risk estimate and it was put into perspective for them as a high, medium, or low risk. Of 25 women at high risk six have had children, all girls; the two in the medium-risk group have had no children; and the 46 women at low risk have had 19 boys and 25 girls. None of the boys has the disease. With detailed counselling most potential carriers of this disease reach decisions in child bearing that are in line with their degree of risk.", "PMID": 963439}
{"id": "pubmed23n0032_5577", "title": "Intracranial aneurysms: analysis of results of microneurosurgery.", "content": "Subarachnoid haemorrhage from intracranial aneurysms has a poor prognosis. Operative management of intracranial aneurysms was once considered ineffective. The first 100 cases treated by micorsurgery were analysed to see whether mortality and morbidity were reduced. Modern surgical techniques halved the total mortality but the morbidity was unaltered. Results can be improved by delaying surgery seven days and by treating any hypertension before surgery.", "contents": "Intracranial aneurysms: analysis of results of microneurosurgery. Subarachnoid haemorrhage from intracranial aneurysms has a poor prognosis. Operative management of intracranial aneurysms was once considered ineffective. The first 100 cases treated by micorsurgery were analysed to see whether mortality and morbidity were reduced. Modern surgical techniques halved the total mortality but the morbidity was unaltered. Results can be improved by delaying surgery seven days and by treating any hypertension before surgery.", "PMID": 963461}
{"id": "pubmed23n0032_5578", "title": "Diagnostic and therapeutic assessment by telephone electrocardiographic monitoring of ambulatory patients.", "content": "The electrocardiograms of ambulatory patients have been monitored over the telephone by staff of the intensive cardiac care unit using equipment in the unit. Telephone monitoring is a useful way of diagnosing transient symptomatic arrhythmias and a reliable aid in supervising the patient's rhythm at the beginning or end of treatment. The doctor has direct contact with the patient at the time of his symptoms so that he can reassure or instruct him. This system costs relatively little in manpower and equipment and permits relatively long periods of follow-up. It is effective, however, only in symptomatic cases in which the rate or rhythm disturbances last long enough to be transmitted. Also important are the negative findings when the patient complains of symptoms and abnormal findings during routine telephone transmissions. Accurate detection of transient ischaemic changes seems to be less reliable, and further technical improvements are required.", "contents": "Diagnostic and therapeutic assessment by telephone electrocardiographic monitoring of ambulatory patients. The electrocardiograms of ambulatory patients have been monitored over the telephone by staff of the intensive cardiac care unit using equipment in the unit. Telephone monitoring is a useful way of diagnosing transient symptomatic arrhythmias and a reliable aid in supervising the patient's rhythm at the beginning or end of treatment. The doctor has direct contact with the patient at the time of his symptoms so that he can reassure or instruct him. This system costs relatively little in manpower and equipment and permits relatively long periods of follow-up. It is effective, however, only in symptomatic cases in which the rate or rhythm disturbances last long enough to be transmitted. Also important are the negative findings when the patient complains of symptoms and abnormal findings during routine telephone transmissions. Accurate detection of transient ischaemic changes seems to be less reliable, and further technical improvements are required.", "PMID": 963462}
{"id": "pubmed23n0032_5579", "title": "A new look at the neonatal electrocardiogram.", "content": "Among 818 newborn babies whose electrocardiograms (ECGs) were recorded between April 1975 and April 1976 were 57 babies with recordings that fell outside the accepted normal range. Preliminary observations have identified the need to define more clearly the range of normal variation. Twelve babies showed asymptomatic conduction abnormalities, three of which were thought sufficiently serious to need treatment. Two babies died suddenly, one of whom had abnormal conduction on the ECG and histologically demonstrated abnormalities of the conducting system. This continuing prospective study may indicate a link between conducting tissue abnormalities and the sudden infant death syndrome. Ten babies had congenital cardiac anomalies, four of which were first discovered because of an abnormal screening cardiogram. Electrocardiography, a simple and noninvasive procedure, may be a valuable addition to the routine neonatal examination.", "contents": "A new look at the neonatal electrocardiogram. Among 818 newborn babies whose electrocardiograms (ECGs) were recorded between April 1975 and April 1976 were 57 babies with recordings that fell outside the accepted normal range. Preliminary observations have identified the need to define more clearly the range of normal variation. Twelve babies showed asymptomatic conduction abnormalities, three of which were thought sufficiently serious to need treatment. Two babies died suddenly, one of whom had abnormal conduction on the ECG and histologically demonstrated abnormalities of the conducting system. This continuing prospective study may indicate a link between conducting tissue abnormalities and the sudden infant death syndrome. Ten babies had congenital cardiac anomalies, four of which were first discovered because of an abnormal screening cardiogram. Electrocardiography, a simple and noninvasive procedure, may be a valuable addition to the routine neonatal examination.", "PMID": 963463}
{"id": "pubmed23n0032_5580", "title": "Cutaneous ectopic schistosomiasis.", "content": "Two cases of cutaneous schistosomiasis due to ectopic ova have recently been seen. Both patients presented with abdominal papular lesions, which were found on biopsy to contain schistosoma ova. To reach these abdominal sites mature worms probably migrate from the portal circulation to the paraumbilical veins, where they anastomose with veins of the caval system.", "contents": "Cutaneous ectopic schistosomiasis. Two cases of cutaneous schistosomiasis due to ectopic ova have recently been seen. Both patients presented with abdominal papular lesions, which were found on biopsy to contain schistosoma ova. To reach these abdominal sites mature worms probably migrate from the portal circulation to the paraumbilical veins, where they anastomose with veins of the caval system.", "PMID": 963464}
{"id": "pubmed23n0032_5581", "title": "Career preferences of doctors graduating in 1974.", "content": "Questionnaires were sent to all 2348 doctors who had graduated from medical schools in England, Scotland, and Wales in 1974 asking about their career preferences. Most were in their second preregistration post, and the response rate was 86-1%. The most popular first choice of career was general practice (665 of the responders; 32-9%), followed by medicine (454; 22-5%), surgery (321; 15-9%), and paediatrics (129; 6-4%). Only 507 of the responders (25-1%), however, stated that their preference was \"definite\". First choices differed widely between men and women graduates and between graduates of different medical schools.", "contents": "Career preferences of doctors graduating in 1974. Questionnaires were sent to all 2348 doctors who had graduated from medical schools in England, Scotland, and Wales in 1974 asking about their career preferences. Most were in their second preregistration post, and the response rate was 86-1%. The most popular first choice of career was general practice (665 of the responders; 32-9%), followed by medicine (454; 22-5%), surgery (321; 15-9%), and paediatrics (129; 6-4%). Only 507 of the responders (25-1%), however, stated that their preference was \"definite\". First choices differed widely between men and women graduates and between graduates of different medical schools.", "PMID": 963468}
{"id": "pubmed23n0032_5582", "title": "Animal model studies of genital chlamydial infections. Immunity to re-infection with guinea-pig inclusion conjunctivitis agent in the urethra and eye of male guinea-pigs.", "content": "A previous report demonstrated that male guinea-pigs could be infected in the urethra with guinea-pig inclusion conjunctivitis (GPIC) agent and that the infection was transmitted during mating from infected males to females. In the experiments reported here, inoculation of male guinea-pigs in the urethra with GPIC organisms resulted in infection which subsided spontaneously in about 2 weeks. Males were demonstrated to be completely resistant to urethral challenge with 10(3)ID50 when tested 6 weeks after urethral infection. These guinea-pigs, immune to re-infection of the urethra, remained susceptible to infection of the eye, but this ocular infection was shorter in duration than that in previously uninfected control animals. Infection in the eye resulted in immunity to both ocular and urethral infection when animals were challenged 6 weeks after the ocular infection.", "contents": "Animal model studies of genital chlamydial infections. Immunity to re-infection with guinea-pig inclusion conjunctivitis agent in the urethra and eye of male guinea-pigs. A previous report demonstrated that male guinea-pigs could be infected in the urethra with guinea-pig inclusion conjunctivitis (GPIC) agent and that the infection was transmitted during mating from infected males to females. In the experiments reported here, inoculation of male guinea-pigs in the urethra with GPIC organisms resulted in infection which subsided spontaneously in about 2 weeks. Males were demonstrated to be completely resistant to urethral challenge with 10(3)ID50 when tested 6 weeks after urethral infection. These guinea-pigs, immune to re-infection of the urethra, remained susceptible to infection of the eye, but this ocular infection was shorter in duration than that in previously uninfected control animals. Infection in the eye resulted in immunity to both ocular and urethral infection when animals were challenged 6 weeks after the ocular infection.", "PMID": 963491}
{"id": "pubmed23n0032_5583", "title": "Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons.", "content": "The isolation rate of Ureaplasma urealyticum was 57 per cent. from 47 patients with non-gonococcal urethritis, 40 per cent. from forty patients with gonorrhoea, 47 per cent from healthy sailors, and 53 per cent. from healthy students and laboratory technicians. No difference in distribution of eight serotypes among 45 isolates from these groups was demonstrated and no particular serotype could be correlated with disease symptoms.", "contents": "Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons. The isolation rate of Ureaplasma urealyticum was 57 per cent. from 47 patients with non-gonococcal urethritis, 40 per cent. from forty patients with gonorrhoea, 47 per cent from healthy sailors, and 53 per cent. from healthy students and laboratory technicians. No difference in distribution of eight serotypes among 45 isolates from these groups was demonstrated and no particular serotype could be correlated with disease symptoms.", "PMID": 963492}
{"id": "pubmed23n0032_5584", "title": "Treatment of trichomoniasis in females with and without gonorrhoea.", "content": "46 women with trichomoniasis and gonorrhea were treated with nimorazole (300 mg twice daily for 7 days) and a trichomonal cure rate of 90-2 per cent. was noted. In addition, 53 of 112 patients with trichomoniasis but without gonorrhoea, were treated with metronidazole (200 mg three times a day for 7 days) and 59 with nimorazole (300 mg twice daily for 7 days) in a randomized trial. The cure rates were 96 and 90-5 per cent. respectively. There was no significant difference in the results noted in the two groups. Neither the trichomonal infection itself nor the result of antitrichomonal therapy was affected by antigonococcal therapy (one-day treatment with ampicillin or single-dose therapy with doxycycline). No increase was noted in the frequency of candidiasis after antitrichomonal therapy (in patients without gonorrhoea) or after antigonococcal therapy, but there was substantial variation between consecutive specimens in the prevalence of C. albicans.", "contents": "Treatment of trichomoniasis in females with and without gonorrhoea. 46 women with trichomoniasis and gonorrhea were treated with nimorazole (300 mg twice daily for 7 days) and a trichomonal cure rate of 90-2 per cent. was noted. In addition, 53 of 112 patients with trichomoniasis but without gonorrhoea, were treated with metronidazole (200 mg three times a day for 7 days) and 59 with nimorazole (300 mg twice daily for 7 days) in a randomized trial. The cure rates were 96 and 90-5 per cent. respectively. There was no significant difference in the results noted in the two groups. Neither the trichomonal infection itself nor the result of antitrichomonal therapy was affected by antigonococcal therapy (one-day treatment with ampicillin or single-dose therapy with doxycycline). No increase was noted in the frequency of candidiasis after antitrichomonal therapy (in patients without gonorrhoea) or after antigonococcal therapy, but there was substantial variation between consecutive specimens in the prevalence of C. albicans.", "PMID": 963493}
{"id": "pubmed23n0032_5585", "title": "Effects of x-radiation on the spinal cord: an experimental study of the morphological changes in central nerve fibres.", "content": "The morphological changes in central nerve fibres after irradiation have been studied in the spinal cord of young adult rats exposed to 100-6 000 rad doses of 250 kV x-rays using the technique of single fibre teasing as well as conventional light and electronmicroscopic examination of cord sections. Two groups of degenerative changes were found in myelinated fibres. The first consisted of breakdown of paranodal myelin and nodal widening. These changes were found as early as two weeks after exposure to 500-6 000 rad doses and increased in frequency with dose and time in the first two months after irradiation. Paranodal myelin breakdown was less frequent after two months but nodal widening was more prominent. Increasing numbers of thinly myelinated fibres were found after three months suggesting that paranodal demyelination was followed by remyelination. This early group of changes confined to the myelin sheath provides a possible pathological basis for the self-limited sensory syndrome which sometimes occurs within a few weeks or months of irradiation of the spinal cord in man. The second type of change which was also first detected as early as two weeks after irradiation and which appears to be unrelated to the first, consisted of random Wallerian-type degeneration of fibres of all calibres in the spinal white matter. The number of affected fibres was initially small but increased with time and there was no clear dose relationship. This random fibre degeneration is probably the forerunner of the later-occurring delayed radionecrosis of the spinal cord which other workers have found to occur with a latent period of up to twelve months after exposure to doses exceeding 1900 rads to the rat.", "contents": "Effects of x-radiation on the spinal cord: an experimental study of the morphological changes in central nerve fibres. The morphological changes in central nerve fibres after irradiation have been studied in the spinal cord of young adult rats exposed to 100-6 000 rad doses of 250 kV x-rays using the technique of single fibre teasing as well as conventional light and electronmicroscopic examination of cord sections. Two groups of degenerative changes were found in myelinated fibres. The first consisted of breakdown of paranodal myelin and nodal widening. These changes were found as early as two weeks after exposure to 500-6 000 rad doses and increased in frequency with dose and time in the first two months after irradiation. Paranodal myelin breakdown was less frequent after two months but nodal widening was more prominent. Increasing numbers of thinly myelinated fibres were found after three months suggesting that paranodal demyelination was followed by remyelination. This early group of changes confined to the myelin sheath provides a possible pathological basis for the self-limited sensory syndrome which sometimes occurs within a few weeks or months of irradiation of the spinal cord in man. The second type of change which was also first detected as early as two weeks after irradiation and which appears to be unrelated to the first, consisted of random Wallerian-type degeneration of fibres of all calibres in the spinal white matter. The number of affected fibres was initially small but increased with time and there was no clear dose relationship. This random fibre degeneration is probably the forerunner of the later-occurring delayed radionecrosis of the spinal cord which other workers have found to occur with a latent period of up to twelve months after exposure to doses exceeding 1900 rads to the rat.", "PMID": 963528}
{"id": "pubmed23n0032_5586", "title": "Progressive encephalomyelitis with rigidity.", "content": "Two cases of encephalomyelitis are described in which the major clinical manifestation was muscular rigidity and stimulus-sensitive muscular spasms. It is suggested, from pathological evidence, that this rigidity was of spinal origin, and that this disorder is a rare but recognizable entity. Comparison is made with previously reported cases of rigidity of spinal origin, including encephalitis lethargica, and with 'subacute myoclonic spinal neuronitis' and the 'stiff man syndrome.'", "contents": "Progressive encephalomyelitis with rigidity. Two cases of encephalomyelitis are described in which the major clinical manifestation was muscular rigidity and stimulus-sensitive muscular spasms. It is suggested, from pathological evidence, that this rigidity was of spinal origin, and that this disorder is a rare but recognizable entity. Comparison is made with previously reported cases of rigidity of spinal origin, including encephalitis lethargica, and with 'subacute myoclonic spinal neuronitis' and the 'stiff man syndrome.'", "PMID": 963529}
{"id": "pubmed23n0032_5587", "title": "Delayed visual perception and delayed visual evoked potentials in the spinal form of multiple sclerosis and in retrobulbar neuritis.", "content": "(1) We have used both subjective and evoked potential tests to study cases of multiple sclerosis with no history of retrobulbar neuritis (spinal patients) and compared them with patients with multiple sclerosis who had experienced an attack of retrobulbar neuritis (RBN). We measured the delay of steady-state evoked potentials (EPs) elicited by flicker in the medium-frequency (13-25 c/s) range, by flicker in the high-frequency (30-60 c/s) range, and by pattern-reversal. We also measured the delay in seeing (perceiving) both an increase of light intensity and a decrease of light intensity. (2) The difference between perceptual delays for the left and right eyes (D s) was abnormal when retrobulbar neuritis affected only one eye (22/22 patients) even when acuity and discs were normal. It might be supposed that this perceptual test would be ineffective when both eyes were affected by retrobulbar neuritis. However, the value of D was abnormal in cases of bilateral retrobulbar neuritis (5/5 patients). Probably the principal reason is that demyelination was patchy in the patients studied. For this same reason the difference between perceptual delays for two sites in the visual field (T s) may also be abnormal. In principle the perceptual delay test can be effective even when both eyes are similarly delayed: abnormal values of T were recorded in 5 spinal patients for whom D was normal. (3) Perceptual delays were measured for an extended group of 19 patients suffering from spinal multiple sclerosis. Taking both D and T into account, the perceptual delay test alone picked out 12/19 spinal patients. The perceptual delay test has the advantage over EP tests that it can detect islands of demyelination as small as 3 degrees diameter, and the apparatus is cheap and straightforward to use. (4) Thirteen patients with spinal multiple sclerosis, including 6 with no ocular signs or symptoms, were examined with a battery of two evoked potential and one perceptual test. Ten patients had clearly abnormal visual delays. Results for the remaining 3 were equivocal. Delay tests can reveal visual damage in most patients who have not experienced an attack of RBN as well as in practically all patients who have experienced an attack. (5) Correlations between the results of the various tests were different in spinal patients and in multiple sclerosis patients who had experienced an attack of retrobulbar neuritis. Flicker EPs, pattern EPs and visual perception were all delayed in every RBN patient, whereas for spinal patients different tests could pick up different patients. Flicker EPs picked up 5/13 spinal patients, pattern EPs 6/13, perceptual delay (D) picked up 4/13 and perceptual delay T picked up 7/13. (6) Delay tests divided spinal multiple sclerosis patients into two fairly distinct groups. In one group pattern EPs and perception were delayed; in the other group flicker EPs were delayed. This grouping corresponded to a clinical distinction between long-standing patients with visual signs and recent patients without visual signs...", "contents": "Delayed visual perception and delayed visual evoked potentials in the spinal form of multiple sclerosis and in retrobulbar neuritis. (1) We have used both subjective and evoked potential tests to study cases of multiple sclerosis with no history of retrobulbar neuritis (spinal patients) and compared them with patients with multiple sclerosis who had experienced an attack of retrobulbar neuritis (RBN). We measured the delay of steady-state evoked potentials (EPs) elicited by flicker in the medium-frequency (13-25 c/s) range, by flicker in the high-frequency (30-60 c/s) range, and by pattern-reversal. We also measured the delay in seeing (perceiving) both an increase of light intensity and a decrease of light intensity. (2) The difference between perceptual delays for the left and right eyes (D s) was abnormal when retrobulbar neuritis affected only one eye (22/22 patients) even when acuity and discs were normal. It might be supposed that this perceptual test would be ineffective when both eyes were affected by retrobulbar neuritis. However, the value of D was abnormal in cases of bilateral retrobulbar neuritis (5/5 patients). Probably the principal reason is that demyelination was patchy in the patients studied. For this same reason the difference between perceptual delays for two sites in the visual field (T s) may also be abnormal. In principle the perceptual delay test can be effective even when both eyes are similarly delayed: abnormal values of T were recorded in 5 spinal patients for whom D was normal. (3) Perceptual delays were measured for an extended group of 19 patients suffering from spinal multiple sclerosis. Taking both D and T into account, the perceptual delay test alone picked out 12/19 spinal patients. The perceptual delay test has the advantage over EP tests that it can detect islands of demyelination as small as 3 degrees diameter, and the apparatus is cheap and straightforward to use. (4) Thirteen patients with spinal multiple sclerosis, including 6 with no ocular signs or symptoms, were examined with a battery of two evoked potential and one perceptual test. Ten patients had clearly abnormal visual delays. Results for the remaining 3 were equivocal. Delay tests can reveal visual damage in most patients who have not experienced an attack of RBN as well as in practically all patients who have experienced an attack. (5) Correlations between the results of the various tests were different in spinal patients and in multiple sclerosis patients who had experienced an attack of retrobulbar neuritis. Flicker EPs, pattern EPs and visual perception were all delayed in every RBN patient, whereas for spinal patients different tests could pick up different patients. Flicker EPs picked up 5/13 spinal patients, pattern EPs 6/13, perceptual delay (D) picked up 4/13 and perceptual delay T picked up 7/13. (6) Delay tests divided spinal multiple sclerosis patients into two fairly distinct groups. In one group pattern EPs and perception were delayed; in the other group flicker EPs were delayed. This grouping corresponded to a clinical distinction between long-standing patients with visual signs and recent patients without visual signs...", "PMID": 963530}
{"id": "pubmed23n0032_5588", "title": "Aluminium, neurofibrillary degeneration and Alzheimer's disease.", "content": "Aluminium is neurotoxic and results in the proliferation of 100 A diameter filaments in the cytoplasm of certain neurons. The aluminium concentration for 7 normal human brains was 1-9 +/- 0-7 SD mug/g dry weight (n = 208 samples). The aluminium content of 585 areas sampled in 10 post-mortem cases of Alzheimer's disease ranging in age from 50 to 88 years, in which the diagnosis was based on the specific histological appearances, revealed an elevated aluminium content in some regions. A range of 0-4 - 107-0 mug/g was encountered and 28 per cent of all regions sampled had concentrations in excess of 4 mug/g. Five of 6 cerebral biopsies from patients with Alzheimer's disease also had elevated aluminium content. In 2 additional Alzheimer's brains with neurofibrillary degeneration restricted to certain anatomical areas, elevated aluminium content was found to be associated with neurofibrillary degeneration and not with senile plaques. Furthermore, elevated aluminium content in multiple cortical regions was not found in 2 vascular dementias of the elderly. While the cytotoxic concentration for human neurons is unknown, the cytotoxic concentration for cat's cerebral neurons appears to lie between 4 and 6 mug/g dry weight.", "contents": "Aluminium, neurofibrillary degeneration and Alzheimer's disease. Aluminium is neurotoxic and results in the proliferation of 100 A diameter filaments in the cytoplasm of certain neurons. The aluminium concentration for 7 normal human brains was 1-9 +/- 0-7 SD mug/g dry weight (n = 208 samples). The aluminium content of 585 areas sampled in 10 post-mortem cases of Alzheimer's disease ranging in age from 50 to 88 years, in which the diagnosis was based on the specific histological appearances, revealed an elevated aluminium content in some regions. A range of 0-4 - 107-0 mug/g was encountered and 28 per cent of all regions sampled had concentrations in excess of 4 mug/g. Five of 6 cerebral biopsies from patients with Alzheimer's disease also had elevated aluminium content. In 2 additional Alzheimer's brains with neurofibrillary degeneration restricted to certain anatomical areas, elevated aluminium content was found to be associated with neurofibrillary degeneration and not with senile plaques. Furthermore, elevated aluminium content in multiple cortical regions was not found in 2 vascular dementias of the elderly. While the cytotoxic concentration for human neurons is unknown, the cytotoxic concentration for cat's cerebral neurons appears to lie between 4 and 6 mug/g dry weight.", "PMID": 963531}
{"id": "pubmed23n0032_5589", "title": "The neuropathology of progressive rubella panencephalitis of late onset.", "content": "The clinicopathological changes in a case of progressive rubella panencephalitis of late onset have been presented. The prominent pathological findings in the brain included diffuse destruction of white matter with perivascular inflammatory cells and gliosis, moderate neuronal loss, numerous amorphous vascular deposits in the white matter and severe generalized cerebellar atrophy. These changes are compared and contrasted with those found in congenital rubella and other latent viral illnesses.", "contents": "The neuropathology of progressive rubella panencephalitis of late onset. The clinicopathological changes in a case of progressive rubella panencephalitis of late onset have been presented. The prominent pathological findings in the brain included diffuse destruction of white matter with perivascular inflammatory cells and gliosis, moderate neuronal loss, numerous amorphous vascular deposits in the white matter and severe generalized cerebellar atrophy. These changes are compared and contrasted with those found in congenital rubella and other latent viral illnesses.", "PMID": 963532}
{"id": "pubmed23n0032_5590", "title": "Benign myopathy, with autosomal dominant inheritance. A report on three pedigrees.", "content": "Three pedigrees are described in which 28 living siblings suffered from a benign myopathy. The first symptoms were observed around the fifth year of life. The proximal muscles were more involved than the distal muscles, the extensors more than the flexors. Due to a marked paresis of the extensor digitorum communis muscles 22 patients showed a flexion contracture of the interphalangeal joints of the last four fingers. In addition 20 patients showed a flexion contracture of the elbows and 12 patients had a plantar flexion contracture of the ankles. A high incidence of congenital torticollis was found. The histopathological features were non-specific and remarkably uniform and consisted of a marked variation in muslce fibre diameter and a very marked increase of fatty tissue. Light-microscopy and electronmicroscopy did not show any specific structural changes. There was normal distribution of type I and type II fibres, without type-grouping or preferential atrophy of one of the fibre types. Lobulated type I fibres were found in 6 out of 12 biopsies. Post-mortem study of one case did not show any convincing features of a neurogenic disorder. As no relationship could be found between the siblings of the 3 pedigrees as far back as the beginning of the eighteenth century, this myopathy seemed to be a new nosological entity.", "contents": "Benign myopathy, with autosomal dominant inheritance. A report on three pedigrees. Three pedigrees are described in which 28 living siblings suffered from a benign myopathy. The first symptoms were observed around the fifth year of life. The proximal muscles were more involved than the distal muscles, the extensors more than the flexors. Due to a marked paresis of the extensor digitorum communis muscles 22 patients showed a flexion contracture of the interphalangeal joints of the last four fingers. In addition 20 patients showed a flexion contracture of the elbows and 12 patients had a plantar flexion contracture of the ankles. A high incidence of congenital torticollis was found. The histopathological features were non-specific and remarkably uniform and consisted of a marked variation in muslce fibre diameter and a very marked increase of fatty tissue. Light-microscopy and electronmicroscopy did not show any specific structural changes. There was normal distribution of type I and type II fibres, without type-grouping or preferential atrophy of one of the fibre types. Lobulated type I fibres were found in 6 out of 12 biopsies. Post-mortem study of one case did not show any convincing features of a neurogenic disorder. As no relationship could be found between the siblings of the 3 pedigrees as far back as the beginning of the eighteenth century, this myopathy seemed to be a new nosological entity.", "PMID": 963533}
{"id": "pubmed23n0032_5591", "title": "Transplantation of central and peripheral monoamine neurons to the adult rat brain: techniques and conditions for survival.", "content": "The conditions for survival of transplants of peripheral and central monoamine neurons in the adult rat brain were studied using fluorescence histochemistry. Pieces of the superior cervical ganglion from newborn and adult rats and CNS tissue containing noradrenaline (NA), dopamine (DA) and indolamine (IA) neurons from embryonic, newborn and adult rats were transplanted to various brain sites using different techniques: insertion of the graft by means of a glass rod, by \"injection\", or by direct implantation of the graft in a resection cavity. Three principally different locations for the graft were tested: within the brain parenchyma in the caudal diencephalon and the caudate nucleus; onto the dorsal surface of the caudate nucleus; and onto the pial covering in the choroidal fissure after removal of the overlying cortex and parts of the hippocampal formation. Attempts were also made to transplant to the dorsal surface of the caudate nucleus or the hippocampus with the aid of an \"artificial\" vascular bed, achieved by previous transplantation of an iris. Consistent survival of the transplanted central and peripheral neurons was obtained only when the transplant was placed in contact with a vessel-rich tissue, such as the pia in the choroidal fissure or the \"artificial\" vascular bed. While the majority of the monoamine-containing neurons in the transplants died within the first month after transplantation, a significant number of neurons (up to about 150 in the ganglionic pieces and up to about 500 in the embryonic or newborn CNS pieces) survived for at least half a year in the brain. Survival of transplanted adult central monoamine neurons was not observed. A substantial outgrowth of axons was observed from all types of neurons in their new location. These newly formed fibers formed extensive fiber patterns within the transplant itself, around pia vessels, and within the adjacent brain tissue, above all in the hippocampal formation. The usefulness of the present transplantation technique for the exploration of mechanisms underlying reformation of axonal connections in the adult mammalian CNS is discussed.", "contents": "Transplantation of central and peripheral monoamine neurons to the adult rat brain: techniques and conditions for survival. The conditions for survival of transplants of peripheral and central monoamine neurons in the adult rat brain were studied using fluorescence histochemistry. Pieces of the superior cervical ganglion from newborn and adult rats and CNS tissue containing noradrenaline (NA), dopamine (DA) and indolamine (IA) neurons from embryonic, newborn and adult rats were transplanted to various brain sites using different techniques: insertion of the graft by means of a glass rod, by \"injection\", or by direct implantation of the graft in a resection cavity. Three principally different locations for the graft were tested: within the brain parenchyma in the caudal diencephalon and the caudate nucleus; onto the dorsal surface of the caudate nucleus; and onto the pial covering in the choroidal fissure after removal of the overlying cortex and parts of the hippocampal formation. Attempts were also made to transplant to the dorsal surface of the caudate nucleus or the hippocampus with the aid of an \"artificial\" vascular bed, achieved by previous transplantation of an iris. Consistent survival of the transplanted central and peripheral neurons was obtained only when the transplant was placed in contact with a vessel-rich tissue, such as the pia in the choroidal fissure or the \"artificial\" vascular bed. While the majority of the monoamine-containing neurons in the transplants died within the first month after transplantation, a significant number of neurons (up to about 150 in the ganglionic pieces and up to about 500 in the embryonic or newborn CNS pieces) survived for at least half a year in the brain. Survival of transplanted adult central monoamine neurons was not observed. A substantial outgrowth of axons was observed from all types of neurons in their new location. These newly formed fibers formed extensive fiber patterns within the transplant itself, around pia vessels, and within the adjacent brain tissue, above all in the hippocampal formation. The usefulness of the present transplantation technique for the exploration of mechanisms underlying reformation of axonal connections in the adult mammalian CNS is discussed.", "PMID": 963534}
{"id": "pubmed23n0032_5592", "title": "Pathways linking L-phenylalanine and 2-phenylethylamine with p-tyramine in rabbit brain.", "content": "Concentrations of labeled p-tyramine and 2-phenylethylamine were measured in rabbit brain 10 min after the intraventricular administration of either radioactive amine. In both cases the recoveries of the newly synthesized amine and of the unchanged precursor were significantly increased in animals pretreated with the monoamine oxidase inhibitor pargyline. Significant amounts of both amines were present in rabbit brain 10 min after the intraventricular injection of labeled L-phenylalanine. Pretreatment with pargyline increased their recoveries, whereas alpha-methyldopa (an L-aromatic amino acid decarboxylase enzyme inhibitor, L-AAADI) decreased them considerably, and no significant alteration was found in L-alpha-methyldopa hydrazine (a peripheral L-AAADI) pretreated animals. These results provide evidence for a new biochemical pathway in brain for p-tyramine biosynthesis, with L-phenylalanine (bypassing the formation of L-tyrosine) or 2-phenylethylamine as precursors. The significance and implications of these metabolic routes are discussed, especially considering that p-tyramine itself can be converted to catecholamines.", "contents": "Pathways linking L-phenylalanine and 2-phenylethylamine with p-tyramine in rabbit brain. Concentrations of labeled p-tyramine and 2-phenylethylamine were measured in rabbit brain 10 min after the intraventricular administration of either radioactive amine. In both cases the recoveries of the newly synthesized amine and of the unchanged precursor were significantly increased in animals pretreated with the monoamine oxidase inhibitor pargyline. Significant amounts of both amines were present in rabbit brain 10 min after the intraventricular injection of labeled L-phenylalanine. Pretreatment with pargyline increased their recoveries, whereas alpha-methyldopa (an L-aromatic amino acid decarboxylase enzyme inhibitor, L-AAADI) decreased them considerably, and no significant alteration was found in L-alpha-methyldopa hydrazine (a peripheral L-AAADI) pretreated animals. These results provide evidence for a new biochemical pathway in brain for p-tyramine biosynthesis, with L-phenylalanine (bypassing the formation of L-tyrosine) or 2-phenylethylamine as precursors. The significance and implications of these metabolic routes are discussed, especially considering that p-tyramine itself can be converted to catecholamines.", "PMID": 963535}
{"id": "pubmed23n0032_5593", "title": "Effect of temperature on postsynaptic potentials of cat spinal motoneurones.", "content": "The effect of spinal cord temperature on excitatory postsynaptic potentials (EPSP) and inhibitory postsynaptic potentials (IPSP) were measured by means of intracellular recordings from lumbar motoneurones of 43 cats. While body temperature and oil bath temperature were maintained between 37 and 38 degrees C, the temperature of the spinal segment under investigation was changed separately in the range between 30 and 42 degrees C. Cooling consistently produced an increase in amplitude and duration of both, mono- and poly-synaptic EPSPs and recurrent and direct IPSPs. Warming caused the opposite effect. The input resistance of the motoneurones was inversely related to the spinal cord temperature, while the latency of action potentials produced by intracellular injection of outward current was directly and exponentially related to spinal temperature. Although the data do not provide a quantitative differentiation of pre- versus postsynaptic temperature effects, they are consistent with the notion that temperature dependent changes on postsynaptic membrane properties contribute to the observed PSP changes. It is further suggested that similar postsynaptic temperature effects may be concerned in temperature sensitivity of proposed specific central neurones.", "contents": "Effect of temperature on postsynaptic potentials of cat spinal motoneurones. The effect of spinal cord temperature on excitatory postsynaptic potentials (EPSP) and inhibitory postsynaptic potentials (IPSP) were measured by means of intracellular recordings from lumbar motoneurones of 43 cats. While body temperature and oil bath temperature were maintained between 37 and 38 degrees C, the temperature of the spinal segment under investigation was changed separately in the range between 30 and 42 degrees C. Cooling consistently produced an increase in amplitude and duration of both, mono- and poly-synaptic EPSPs and recurrent and direct IPSPs. Warming caused the opposite effect. The input resistance of the motoneurones was inversely related to the spinal cord temperature, while the latency of action potentials produced by intracellular injection of outward current was directly and exponentially related to spinal temperature. Although the data do not provide a quantitative differentiation of pre- versus postsynaptic temperature effects, they are consistent with the notion that temperature dependent changes on postsynaptic membrane properties contribute to the observed PSP changes. It is further suggested that similar postsynaptic temperature effects may be concerned in temperature sensitivity of proposed specific central neurones.", "PMID": 963544}
{"id": "pubmed23n0032_5594", "title": "Changes in multiunit activity and EEG induced by the administration of natural progestins to flaxedil immobilized cats.", "content": "The effects of progesterone (P) and 7 of its metabolites on the EEG and multiunit activity (MUA) of various limbic structures were studied in flaxedil immobilized cats. Most progestins inhibited the MUA of the mesencephalic reticular formation, hippocampus and hypothalamus and induced cortical EEG synchronization. MUA changes in the mesencephalic reticular formation frequently preceded EEG synchronization. Progestins reduced in the 5 beta postition (pregnanolone, epipregnanolone and pregnanedion) inhibited neuronal discharge and synchronized EEG activity with shorter latencies and in lower doses than those reduced in the 5 alpha position (allopregnanolone and allopregnanedion). Progestins having the delta 4,3-keto structure (P, 20 alpha-hydroxy pregnenone, 20 beta-hydroxy pregnenone) induced electrophysiological changes with prolonged latencies and in doses significantly higher than ring A reduced progestins. Administration of epipregnanolone to \"cerveau isole\" preparations depressed neuronal firing in those structures located rostral to the level of the brain stem transection (hypothalamus, hippocampus), but failed to increase the already synchronized cortical activity. These results suggest that: a, ring A reduction increases the capacity of P and other delta 4,3-keto progestins to inhibit neuronal discharge in certain brain areas and b, changes in neuronal firing induced by progestins are not necessarily mediated through the cat brain stem reticular formation.", "contents": "Changes in multiunit activity and EEG induced by the administration of natural progestins to flaxedil immobilized cats. The effects of progesterone (P) and 7 of its metabolites on the EEG and multiunit activity (MUA) of various limbic structures were studied in flaxedil immobilized cats. Most progestins inhibited the MUA of the mesencephalic reticular formation, hippocampus and hypothalamus and induced cortical EEG synchronization. MUA changes in the mesencephalic reticular formation frequently preceded EEG synchronization. Progestins reduced in the 5 beta postition (pregnanolone, epipregnanolone and pregnanedion) inhibited neuronal discharge and synchronized EEG activity with shorter latencies and in lower doses than those reduced in the 5 alpha position (allopregnanolone and allopregnanedion). Progestins having the delta 4,3-keto structure (P, 20 alpha-hydroxy pregnenone, 20 beta-hydroxy pregnenone) induced electrophysiological changes with prolonged latencies and in doses significantly higher than ring A reduced progestins. Administration of epipregnanolone to \"cerveau isole\" preparations depressed neuronal firing in those structures located rostral to the level of the brain stem transection (hypothalamus, hippocampus), but failed to increase the already synchronized cortical activity. These results suggest that: a, ring A reduction increases the capacity of P and other delta 4,3-keto progestins to inhibit neuronal discharge in certain brain areas and b, changes in neuronal firing induced by progestins are not necessarily mediated through the cat brain stem reticular formation.", "PMID": 963545}
{"id": "pubmed23n0032_5595", "title": "Systematic examination in the rat of brain sites sensitive to the direct application of morphine: observation of differential effects within the periaqueductal gray.", "content": "An extensive mapping of the rat brain (403 sites) ranging from AP +8 to AP -3 revealed that the region showing maximum sensitivity to the intracerebral administration of morphine in the elevation of the nociceptive threshold lay within the periaqueductal gray. Analysis of the distribution of responsive sites indicated that the most active sites, those having the shortest latency of effect, were located within the ventrolateral aspect of the caudal periaqueductal gray. These antinociceptive actions of morphine were observed to be both dose-dependent and reversible by the administration of naloxone. We observed that microinjections of morphine could produce changes in the pinch withdrawal response which were distributed in a crude somatotopic fashion. Injections into the rostral aspect of the periaqueductal gray yielded a block of the pinch response in the rostral portions of the body, whereas such injections into the caudal periaqueductal gray always yielded a whole body analgesia. In the rostral sites, transient ipsilateral blocks of the pinch response were occasionally seen. A pinch block limited to the hind paws alone was never observed. It is suggested that morphine acting through the periaqueductal gray may actuate a potent supraspinal modulatory system related to the transmission of information derived from behaviorally aversive stimuli.", "contents": "Systematic examination in the rat of brain sites sensitive to the direct application of morphine: observation of differential effects within the periaqueductal gray. An extensive mapping of the rat brain (403 sites) ranging from AP +8 to AP -3 revealed that the region showing maximum sensitivity to the intracerebral administration of morphine in the elevation of the nociceptive threshold lay within the periaqueductal gray. Analysis of the distribution of responsive sites indicated that the most active sites, those having the shortest latency of effect, were located within the ventrolateral aspect of the caudal periaqueductal gray. These antinociceptive actions of morphine were observed to be both dose-dependent and reversible by the administration of naloxone. We observed that microinjections of morphine could produce changes in the pinch withdrawal response which were distributed in a crude somatotopic fashion. Injections into the rostral aspect of the periaqueductal gray yielded a block of the pinch response in the rostral portions of the body, whereas such injections into the caudal periaqueductal gray always yielded a whole body analgesia. In the rostral sites, transient ipsilateral blocks of the pinch response were occasionally seen. A pinch block limited to the hind paws alone was never observed. It is suggested that morphine acting through the periaqueductal gray may actuate a potent supraspinal modulatory system related to the transmission of information derived from behaviorally aversive stimuli.", "PMID": 963546}
{"id": "pubmed23n0032_5596", "title": "A quantitative autoradiographic and electrophysiological study of the reinnervation of the dentate gyrus by the contralateral entorhinal cortex following ipsilateral entorhinal lesions.", "content": "The post-lesion proliferation of contralateral enthorhinal afferents which occurs in response to ipsilateral entorhinal lesions was quantitatively analyzed with autoradiographic and electrophysiological techniques. In both cases, the extent of the crossed projection to the dentate granule cells was quantified on the basis of a contralateral/ipsilateral (C/I) ratio. Autoradiographic measures of grain density in the entorhinal terminal field indicates that the very sparse crossed entorhinal projection in intact animals proliferates approximately 6-fold following unilateral entorhinal lesions (on the basis of an increased C/I ratio of grain density in animals with long standing unilater entorhinal lesions). Furthermore, the total number of grains in the entorhinal terminal zone (obtained by subtracting background from non-terminal regions) also increases approximately 6-fold, indicating that compression of the neuropil cannot be the factor responsible for the increased grain density. These increases in the anatomical extent of the crossed projection as a consequence of unilateral entorhinal lesions are also reflected electrophysiologically. In operated animals, the C/I ratio of the extracellular population EPSP (a measure of the synaptic current generated by the crossed projections) also increase 5-8 fold. In addition, while in normal animals, no population spikes are observed following stimulation of the contralateral entorhinal area (indicating an absence of synchronous grnaule cell discharge in response to contralateral entorhinal input), such population spikes are quite prominent in the reinnervated dentate gyrus, indicating a large increase in the effective synaptic drive of the proliferated crossed projections.", "contents": "A quantitative autoradiographic and electrophysiological study of the reinnervation of the dentate gyrus by the contralateral entorhinal cortex following ipsilateral entorhinal lesions. The post-lesion proliferation of contralateral enthorhinal afferents which occurs in response to ipsilateral entorhinal lesions was quantitatively analyzed with autoradiographic and electrophysiological techniques. In both cases, the extent of the crossed projection to the dentate granule cells was quantified on the basis of a contralateral/ipsilateral (C/I) ratio. Autoradiographic measures of grain density in the entorhinal terminal field indicates that the very sparse crossed entorhinal projection in intact animals proliferates approximately 6-fold following unilateral entorhinal lesions (on the basis of an increased C/I ratio of grain density in animals with long standing unilater entorhinal lesions). Furthermore, the total number of grains in the entorhinal terminal zone (obtained by subtracting background from non-terminal regions) also increases approximately 6-fold, indicating that compression of the neuropil cannot be the factor responsible for the increased grain density. These increases in the anatomical extent of the crossed projection as a consequence of unilateral entorhinal lesions are also reflected electrophysiologically. In operated animals, the C/I ratio of the extracellular population EPSP (a measure of the synaptic current generated by the crossed projections) also increase 5-8 fold. In addition, while in normal animals, no population spikes are observed following stimulation of the contralateral entorhinal area (indicating an absence of synchronous grnaule cell discharge in response to contralateral entorhinal input), such population spikes are quite prominent in the reinnervated dentate gyrus, indicating a large increase in the effective synaptic drive of the proliferated crossed projections.", "PMID": 963547}
{"id": "pubmed23n0032_5597", "title": "Electrophysiological analysis of the projection from the contralateral entorhinal cortex to the dentate gyrus in normal rats.", "content": "The projection from the contralateral entorhinal cortex to the dentate gyrus is shown to exert a monosynaptic excitatory action. Stimulation of the contralateral entorhinal cortex evokes unitary granule cell discharges in the dentate gyrus. This evoked activity is followed by a period of inhibition lasting about 50 msec. Laminar analyses of field potentials generated by contralateral medial entorhinal stimulation localize the synaptic activity to the middle portion of the granule cell dendrites. This localization is consistent with the termination site of the contralateral entorhinal projection as previously shown anatomically. Earlier studies have indicated that the entorhinal cortex excites only the ipsilateral dentate gyrus. These findings now demonstrate that the cortical input to the dentate gyrus is bilateral in normal rats. However, the contralateral projection appears to be much less efficacious than the ipsilateral input.", "contents": "Electrophysiological analysis of the projection from the contralateral entorhinal cortex to the dentate gyrus in normal rats. The projection from the contralateral entorhinal cortex to the dentate gyrus is shown to exert a monosynaptic excitatory action. Stimulation of the contralateral entorhinal cortex evokes unitary granule cell discharges in the dentate gyrus. This evoked activity is followed by a period of inhibition lasting about 50 msec. Laminar analyses of field potentials generated by contralateral medial entorhinal stimulation localize the synaptic activity to the middle portion of the granule cell dendrites. This localization is consistent with the termination site of the contralateral entorhinal projection as previously shown anatomically. Earlier studies have indicated that the entorhinal cortex excites only the ipsilateral dentate gyrus. These findings now demonstrate that the cortical input to the dentate gyrus is bilateral in normal rats. However, the contralateral projection appears to be much less efficacious than the ipsilateral input.", "PMID": 963548}
{"id": "pubmed23n0032_5598", "title": "Discharge patterns of bulbo-pontine respiratory unit populations in cat.", "content": "Respiration related units (RRU) were recorded during a stratigraphic exploration of medulla and pons from the cervical junction to the caudal part of the pneumotaxic system in the semi-chronic locally anesthetized 'isolated respiratory centre' of the cat. Metal 'low impedance, capacitance compensated' microelectrodes recorded multi-unit signals from which unitary discharges were discriminated and processed by computer; it is suggested that using these techniques, the sample was a good representation of the total unit population. The phase relation to phrenic discharge was determined on cycle triggered time histograms. Of 23,000 units, 28% had a definite respiratory modulation. Examined individually, each RRU showed a stable discharge pattern corresponding to one of various respiratory types, the majority of which have been described previously. Both temporal and spatial distributions of RRU discharges were analyzed. Temporal distribution of peak firing frequencies (PFF) of 5,000 RRU sampled anatomically at random showed two main populations whose modes were observed during inspiration (I) or expiration (E). Troughs were observed in the histogram at the transition from I to E and E to I, thus indicating low probability for finding phase spanning RRU in the medulla and pons up to the pneumotaxic level. These statistical results turned out to be identical to those obtained with an a priori classification method comparable to that used in most of the previous works. In addition, the PFF distribution suggested that the E population could be further divided into 3 sub-populations whose modes fall in early, mid, and late expiration respectively. Comparison of RRU temporal distribution in two regions, one rostral, another caudal to a frontal Horsley-Clarke plane situated 3 mm in front of the obex, showed that, in the caudal region, 70% of the RRU were I units, while, in the rostral medulla and pons, equal proportions of I and E neurons were found. Temporal distribution of RRU peak frequencies was studied separately in anatomical structures where the probability of finding RRU was high. No clear correspondence between RRU types and anatomy could be found, but marked differences between structures were observed, thus suggesting nevertheless a different spatial distribution for I and E populations.", "contents": "Discharge patterns of bulbo-pontine respiratory unit populations in cat. Respiration related units (RRU) were recorded during a stratigraphic exploration of medulla and pons from the cervical junction to the caudal part of the pneumotaxic system in the semi-chronic locally anesthetized 'isolated respiratory centre' of the cat. Metal 'low impedance, capacitance compensated' microelectrodes recorded multi-unit signals from which unitary discharges were discriminated and processed by computer; it is suggested that using these techniques, the sample was a good representation of the total unit population. The phase relation to phrenic discharge was determined on cycle triggered time histograms. Of 23,000 units, 28% had a definite respiratory modulation. Examined individually, each RRU showed a stable discharge pattern corresponding to one of various respiratory types, the majority of which have been described previously. Both temporal and spatial distributions of RRU discharges were analyzed. Temporal distribution of peak firing frequencies (PFF) of 5,000 RRU sampled anatomically at random showed two main populations whose modes were observed during inspiration (I) or expiration (E). Troughs were observed in the histogram at the transition from I to E and E to I, thus indicating low probability for finding phase spanning RRU in the medulla and pons up to the pneumotaxic level. These statistical results turned out to be identical to those obtained with an a priori classification method comparable to that used in most of the previous works. In addition, the PFF distribution suggested that the E population could be further divided into 3 sub-populations whose modes fall in early, mid, and late expiration respectively. Comparison of RRU temporal distribution in two regions, one rostral, another caudal to a frontal Horsley-Clarke plane situated 3 mm in front of the obex, showed that, in the caudal region, 70% of the RRU were I units, while, in the rostral medulla and pons, equal proportions of I and E neurons were found. Temporal distribution of RRU peak frequencies was studied separately in anatomical structures where the probability of finding RRU was high. No clear correspondence between RRU types and anatomy could be found, but marked differences between structures were observed, thus suggesting nevertheless a different spatial distribution for I and E populations.", "PMID": 963549}
{"id": "pubmed23n0032_5599", "title": "Three dimensional representation of bulbo-pontine respiratory networks architecture from unit density maps.", "content": "Results of the preceding paper32 suggest that bulbo-pontine respiration related units (RRU) could be classed into 4 populations according to the relation of their peak firing frequency to the respiratory cycle: one inspiratory (I) and 3 expiratory (E) (early, mid and late). A further study of their spatial distribution is reported. Recorded units totalling 26,520 were distributed through a matrix of unitary cubes of which about 2,000 were found within the limits of the medulla and pons. Both percentage and density of respiratory and non-respiratory units were estimated in each cube. Statistical analysis of unit distribution showed a high probability of finding RRU in certain regions. The probability of finding I and E units in separate cubes was high, whereas there was no probability better than chance for recording one of the 3 E types separately from the 2 others. Therefore, only two populations, I and E, could be considered for spatial analysis. A thresholding technique applied to I and E unit density figures in unitary cubes demonstrated a definite limit for I and E populations. A computerized, three-dimensional (3-D) reconstruction of the outer surfaces of the I and E networks showed that half a medullary system consisted of 2 contiguous and uninterrupted columns extending from the cervical junction up to the pneumotaxic system. The main body of the I population was found in the caudal third and dorsolateral part of the bulbopontine region, while the main body of the E population was in the middle third in a ventromedial position. Both have caudal and rostral extensions that run parallel to the main axis of the brainstem and give rise to several lateral branches; these lie in close contact and interdigitate extensively, especially in the rostral third of the bulbopontine region. From the outer limits of I and E networks, a progressive increase of unit density levels was observed towards the core; it showed that inside both I and E populations, RRU aggregate into high density foci (HDF). Large HDF were found in the main body of each population. Smaller HDF were observed scattered throughout the medulla; I, and to a lesser extent E, HDF invade and surround sensory and motor nuclei and roots of the branchial nerves.", "contents": "Three dimensional representation of bulbo-pontine respiratory networks architecture from unit density maps. Results of the preceding paper32 suggest that bulbo-pontine respiration related units (RRU) could be classed into 4 populations according to the relation of their peak firing frequency to the respiratory cycle: one inspiratory (I) and 3 expiratory (E) (early, mid and late). A further study of their spatial distribution is reported. Recorded units totalling 26,520 were distributed through a matrix of unitary cubes of which about 2,000 were found within the limits of the medulla and pons. Both percentage and density of respiratory and non-respiratory units were estimated in each cube. Statistical analysis of unit distribution showed a high probability of finding RRU in certain regions. The probability of finding I and E units in separate cubes was high, whereas there was no probability better than chance for recording one of the 3 E types separately from the 2 others. Therefore, only two populations, I and E, could be considered for spatial analysis. A thresholding technique applied to I and E unit density figures in unitary cubes demonstrated a definite limit for I and E populations. A computerized, three-dimensional (3-D) reconstruction of the outer surfaces of the I and E networks showed that half a medullary system consisted of 2 contiguous and uninterrupted columns extending from the cervical junction up to the pneumotaxic system. The main body of the I population was found in the caudal third and dorsolateral part of the bulbopontine region, while the main body of the E population was in the middle third in a ventromedial position. Both have caudal and rostral extensions that run parallel to the main axis of the brainstem and give rise to several lateral branches; these lie in close contact and interdigitate extensively, especially in the rostral third of the bulbopontine region. From the outer limits of I and E networks, a progressive increase of unit density levels was observed towards the core; it showed that inside both I and E populations, RRU aggregate into high density foci (HDF). Large HDF were found in the main body of each population. Smaller HDF were observed scattered throughout the medulla; I, and to a lesser extent E, HDF invade and surround sensory and motor nuclei and roots of the branchial nerves.", "PMID": 963550}
{"id": "pubmed23n0032_5600", "title": "Acid phosphatase in rat neurohypophyseal dispersions and its fractions enriched for neurosecretosomes and pituicytes after water deprivation and lactation.", "content": "Neurohypophyseal dispersions and fractions enriched for neurosecretosomes and pituicytes were prepared from rats subjected to 6 days of water deprivation and 9-10 days of lactation as stimuli of the hypothalamo-neurohypophyseal system (HNS). After water deprivation the content of the fractions changed in such a way that the neurosecretosomes, and to a lesser extent also the pituicytes, accumulated at a lower density within the gradient used for separation. Stimulation by means of lactation did not show such changes when a comparison was made with dioestrus. Microchemical and histochemical tests for acid phosphatase showed that most of the activity in the controls was present in the neurosecretosomes. A rough calculation, which takes into account the different yields for the dispersion elements, showed a rather equal distribution for acid phosphatase activity between axonal and pituicytic compartments of the intact neurohypophysis. The known acid phosphatase activity response of the neural lobe after HNS stimulation, which was also detectable in the dispersion, resulted histochemically in an increased staining intensity for both neurosecretosomes and pituicytes, but with microassay it was distributed along a gradient similar to oxytocin. It was therefore concluded that this lysosomal enzyme response within the neurophypophysis is preferentially localized in the neurohypophysis is preferentially localized in the neurosecretory axons.", "contents": "Acid phosphatase in rat neurohypophyseal dispersions and its fractions enriched for neurosecretosomes and pituicytes after water deprivation and lactation. Neurohypophyseal dispersions and fractions enriched for neurosecretosomes and pituicytes were prepared from rats subjected to 6 days of water deprivation and 9-10 days of lactation as stimuli of the hypothalamo-neurohypophyseal system (HNS). After water deprivation the content of the fractions changed in such a way that the neurosecretosomes, and to a lesser extent also the pituicytes, accumulated at a lower density within the gradient used for separation. Stimulation by means of lactation did not show such changes when a comparison was made with dioestrus. Microchemical and histochemical tests for acid phosphatase showed that most of the activity in the controls was present in the neurosecretosomes. A rough calculation, which takes into account the different yields for the dispersion elements, showed a rather equal distribution for acid phosphatase activity between axonal and pituicytic compartments of the intact neurohypophysis. The known acid phosphatase activity response of the neural lobe after HNS stimulation, which was also detectable in the dispersion, resulted histochemically in an increased staining intensity for both neurosecretosomes and pituicytes, but with microassay it was distributed along a gradient similar to oxytocin. It was therefore concluded that this lysosomal enzyme response within the neurophypophysis is preferentially localized in the neurohypophysis is preferentially localized in the neurosecretory axons.", "PMID": 963551}
{"id": "pubmed23n0032_5601", "title": "Sleep and motor activity of the rat during ultra-short light-dark cycles.", "content": "The vigilance states and motor activity of the rat were recorded during ultrashort light-dark (LD) cycles. The LD schedules were the following: 10 min light -- 10 min dark (LD 10:10), LD 7.5:7.5, LD 5:5 and LD 2.5:2.5. With all short LD schedules, paradoxical sleep (PS) and high motor activity occurred more frequently during dark periods than during light. PS and motor activity were rapidly enhanced by darkness and inhibited by light, whereas the percentages of total sleep and slow wave sleep were only gradually decreased during the dark periods and increased during light. Compared to the LD 12h:12h control days, sleep was enhanced by all short LD schedules, an effect that was most marked in the circadian phase of maximum waking (corresponding to the LD 12h:12h dark period). The short LD cycles interacted with an intrinsic sleep cycle, whose period is approximately 10 min. Interval histograms of PS episodes showed that an optimal synchronization of the ultradian sleep rhythm is achieved when the period of the LD cycle or its multiple correspond also to 10 min. It is suggested that the response of PS and motor activity to changes in illumination may be due to common mechanisms.", "contents": "Sleep and motor activity of the rat during ultra-short light-dark cycles. The vigilance states and motor activity of the rat were recorded during ultrashort light-dark (LD) cycles. The LD schedules were the following: 10 min light -- 10 min dark (LD 10:10), LD 7.5:7.5, LD 5:5 and LD 2.5:2.5. With all short LD schedules, paradoxical sleep (PS) and high motor activity occurred more frequently during dark periods than during light. PS and motor activity were rapidly enhanced by darkness and inhibited by light, whereas the percentages of total sleep and slow wave sleep were only gradually decreased during the dark periods and increased during light. Compared to the LD 12h:12h control days, sleep was enhanced by all short LD schedules, an effect that was most marked in the circadian phase of maximum waking (corresponding to the LD 12h:12h dark period). The short LD cycles interacted with an intrinsic sleep cycle, whose period is approximately 10 min. Interval histograms of PS episodes showed that an optimal synchronization of the ultradian sleep rhythm is achieved when the period of the LD cycle or its multiple correspond also to 10 min. It is suggested that the response of PS and motor activity to changes in illumination may be due to common mechanisms.", "PMID": 963552}
{"id": "pubmed23n0032_5602", "title": "Methionine supplementation of low protein diets for broiler chicks in the tropics.", "content": "In the first experiment, diets containing 14, 16, 18, 20, 22 or 24% protein with no methionine supplementation were tested, while in the second experiment diets containing 16, 18 and 20% supplemented with methionine to give 90, 100 or 110% of the recommended N.R.C. levels were compared with an unsupplemented 24% protein diet. 2. Methionine supplementation of the low protein diets stimulated faster gains than the unsupplemented diets but not enough to surpass the response to the 24% protein control diet. 3. It was concluded that a high protein diet unsupplemented with methionine was more economical to feed to broilers than supplemented low protein diets.", "contents": "Methionine supplementation of low protein diets for broiler chicks in the tropics. In the first experiment, diets containing 14, 16, 18, 20, 22 or 24% protein with no methionine supplementation were tested, while in the second experiment diets containing 16, 18 and 20% supplemented with methionine to give 90, 100 or 110% of the recommended N.R.C. levels were compared with an unsupplemented 24% protein diet. 2. Methionine supplementation of the low protein diets stimulated faster gains than the unsupplemented diets but not enough to surpass the response to the 24% protein control diet. 3. It was concluded that a high protein diet unsupplemented with methionine was more economical to feed to broilers than supplemented low protein diets.", "PMID": 963567}
{"id": "pubmed23n0032_5603", "title": "The effect of short heat stress upon leucocyte count, plasma corticosterone level, plasma and leucocyte ascorbic acid content.", "content": "Cocks were exposed to 1 or 2 h at 24 degrees C; blood samples were taken before and immediately after treatment and for a period of 20 h thereafter. 2. Heat caused a significant decrease in leucocyte counts, an increase in corticosterone concentration in plasma and an increase of ascorbic acid in plasma and in leucocytes. 3. These four measures can be used to indicate a stress response and the measurements can be done without harming the animal.", "contents": "The effect of short heat stress upon leucocyte count, plasma corticosterone level, plasma and leucocyte ascorbic acid content. Cocks were exposed to 1 or 2 h at 24 degrees C; blood samples were taken before and immediately after treatment and for a period of 20 h thereafter. 2. Heat caused a significant decrease in leucocyte counts, an increase in corticosterone concentration in plasma and an increase of ascorbic acid in plasma and in leucocytes. 3. These four measures can be used to indicate a stress response and the measurements can be done without harming the animal.", "PMID": 963568}
{"id": "pubmed23n0032_5604", "title": "Inheritance of body weight and rate of gain in Japanese quail.", "content": "The effects of hatch date, location and sex on the body weight and rate of gain in Coturnix coturnix japonica were examined. 2. The data conformed to a mixed and adjustments were made for the fixed effects sex, hatch date and location. Adjustment improved the precision of the estimates by reducing the error variances significantly. 3. Rearing equal numbers of birds from each family at different locations made the interactions negligible. 4. Weight gain was greater if illumination intensity up to 35 d was less. 5. Females were not heavier on the 1st day but were heavier on the 14th and 35th days than males. 6. Variance components and genetic parameters were estimated.", "contents": "Inheritance of body weight and rate of gain in Japanese quail. The effects of hatch date, location and sex on the body weight and rate of gain in Coturnix coturnix japonica were examined. 2. The data conformed to a mixed and adjustments were made for the fixed effects sex, hatch date and location. Adjustment improved the precision of the estimates by reducing the error variances significantly. 3. Rearing equal numbers of birds from each family at different locations made the interactions negligible. 4. Weight gain was greater if illumination intensity up to 35 d was less. 5. Females were not heavier on the 1st day but were heavier on the 14th and 35th days than males. 6. Variance components and genetic parameters were estimated.", "PMID": 963569}
{"id": "pubmed23n0032_5605", "title": "On estimating non-additive genetic parameters in chickens.", "content": "Several economically important traits in two Leghorn populations (over 9000 birds) were examined for additive and non-additive components of genetic variance and sex-linked effects. Data were analysed by two different statistical models based on least-squares procedures. 2. Six different covariances were first calculated between relatives; i.e., full-sibs 3/4-sisters, half-sisters, dam-daughters, grandam-granddaughters and aunt-nieces. 3. From the covariances, weighted least-squares equations were used to obtain estimates of variance components for additive genetic, dominance, maternal and sex-linkage effects. 4. The estimates of non-additive components were highly variable but generally small compared with the additive genetic estimates. 5. In general this study suggests that for most traits, with the possible exception of rate of egg production, there is relatively little non-additive genetic variation. 6. The consequences of possible negative correlations between additive effects and maternal effects are considered as they might apply to egg production in poultry.", "contents": "On estimating non-additive genetic parameters in chickens. Several economically important traits in two Leghorn populations (over 9000 birds) were examined for additive and non-additive components of genetic variance and sex-linked effects. Data were analysed by two different statistical models based on least-squares procedures. 2. Six different covariances were first calculated between relatives; i.e., full-sibs 3/4-sisters, half-sisters, dam-daughters, grandam-granddaughters and aunt-nieces. 3. From the covariances, weighted least-squares equations were used to obtain estimates of variance components for additive genetic, dominance, maternal and sex-linkage effects. 4. The estimates of non-additive components were highly variable but generally small compared with the additive genetic estimates. 5. In general this study suggests that for most traits, with the possible exception of rate of egg production, there is relatively little non-additive genetic variation. 6. The consequences of possible negative correlations between additive effects and maternal effects are considered as they might apply to egg production in poultry.", "PMID": 963570}
{"id": "pubmed23n0032_5606", "title": "Method of colostomy and cannulation of the chicken.", "content": "A procedure for colostomising birds from 3 weeks to 5 months of age is described. 2. A plastic cannula was inserted into the fistula 10 d after surgery to keep it open. 3. Provided that the cannula was changed regularly at 10-d intervals there was no constipation or dilation of the intestine.", "contents": "Method of colostomy and cannulation of the chicken. A procedure for colostomising birds from 3 weeks to 5 months of age is described. 2. A plastic cannula was inserted into the fistula 10 d after surgery to keep it open. 3. Provided that the cannula was changed regularly at 10-d intervals there was no constipation or dilation of the intestine.", "PMID": 963571}
{"id": "pubmed23n0032_5607", "title": "Heritabilities and genetic correlations in related dwarf and normal broiler populations.", "content": "Heritability estimates were higher for 8-week body weight in dwarf than in normal broiler populations due to the maternal effects of dwarf dams. 2. The dwarfing gene dw did not induce new genetic variability for egg weight, 30-week body weight and age at sexual maturation. 3. Genetic correlation estimates showed that the selection for 8-week body weight will increase egg weight in dwarf pullets more than in normals. 4. Within a dwarf population it should be possible to increase 8-week body weight without influencing the mature size of pullets.", "contents": "Heritabilities and genetic correlations in related dwarf and normal broiler populations. Heritability estimates were higher for 8-week body weight in dwarf than in normal broiler populations due to the maternal effects of dwarf dams. 2. The dwarfing gene dw did not induce new genetic variability for egg weight, 30-week body weight and age at sexual maturation. 3. Genetic correlation estimates showed that the selection for 8-week body weight will increase egg weight in dwarf pullets more than in normals. 4. Within a dwarf population it should be possible to increase 8-week body weight without influencing the mature size of pullets.", "PMID": 963572}
{"id": "pubmed23n0032_5608", "title": "Direct methods for the analysis of lamellar neutron diffraction from oriented multilayers: a difference Patterson deconvolution approach.", "content": "We have described here a direct method for the solution of the phase problem for lamellar neutron diffraction from hydrated oriented multilayers of model membranes. As a result, the neutron-scattering profiles of such model membranes can be uniquely determined. We note that such methods can easily be extended to cases in which the thickness of the water layer w is somewhat greater than d/2 as long as it is substantially less than d [e.g., w less than 3d/4 would suffice, since deltaP(-d/4 less than or equal to x less than or equal to d/4) would contain only contributions from see article without overlapping contributions from see articles and to cases in which isotopic labeling is used instead of partial H2O-D2O exchange.", "contents": "Direct methods for the analysis of lamellar neutron diffraction from oriented multilayers: a difference Patterson deconvolution approach. We have described here a direct method for the solution of the phase problem for lamellar neutron diffraction from hydrated oriented multilayers of model membranes. As a result, the neutron-scattering profiles of such model membranes can be uniquely determined. We note that such methods can easily be extended to cases in which the thickness of the water layer w is somewhat greater than d/2 as long as it is substantially less than d [e.g., w less than 3d/4 would suffice, since deltaP(-d/4 less than or equal to x less than or equal to d/4) would contain only contributions from see article without overlapping contributions from see articles and to cases in which isotopic labeling is used instead of partial H2O-D2O exchange.", "PMID": 963575}
{"id": "pubmed23n0032_5609", "title": "The structure of carbon monoxide myoglobin: real-space refinement.", "content": "The refinement results in optimum positioning of atoms in the density in most cases, and the juxtaposition of positive and negative density leads to high contrast in the maps and rapid convergence of the refinement. The real-space technique is particularly useful in handling the problem of peak overlap, which is difficult in the neutron case. All refinements to date have been done on the original map, which was phased without hydrogen and deuterium atoms, but the next series of refinements will contain H and D atoms, hydrogen exchange information, and structured water molecules. Comparison of the refinements of CO, Mb and met Mb have shown the iron atom to move nearly into the heme plane. No deuterium atom exists to bond the distal histidine to the CO ligand. Atomic coordinates of all atoms and water molecules will be published on completion of the refinements.", "contents": "The structure of carbon monoxide myoglobin: real-space refinement. The refinement results in optimum positioning of atoms in the density in most cases, and the juxtaposition of positive and negative density leads to high contrast in the maps and rapid convergence of the refinement. The real-space technique is particularly useful in handling the problem of peak overlap, which is difficult in the neutron case. All refinements to date have been done on the original map, which was phased without hydrogen and deuterium atoms, but the next series of refinements will contain H and D atoms, hydrogen exchange information, and structured water molecules. Comparison of the refinements of CO, Mb and met Mb have shown the iron atom to move nearly into the heme plane. No deuterium atom exists to bond the distal histidine to the CO ligand. Atomic coordinates of all atoms and water molecules will be published on completion of the refinements.", "PMID": 963577}
{"id": "pubmed23n0032_5610", "title": "Use of the tangent formula for the refinement of neutron protein data.", "content": "The tangent refinement technique has been shown to work marginally for protein neutron data, at least to the extent that the images of all atoms except hydrogen are sharpened. However, the process is very sensitive to over-refinement, and all the maps were sharpened excessively. Refinements in the squared structure were more stable, and the maps were somewhat better than those from the original structure refinement. This technique might be helpful in certain instances, but the poor quality of the maps prevents it from being generally useful. Perhaps the tangent refinement technique will be more fruitful in application to neutron protein data when experimental phase information is available.", "contents": "Use of the tangent formula for the refinement of neutron protein data. The tangent refinement technique has been shown to work marginally for protein neutron data, at least to the extent that the images of all atoms except hydrogen are sharpened. However, the process is very sensitive to over-refinement, and all the maps were sharpened excessively. Refinements in the squared structure were more stable, and the maps were somewhat better than those from the original structure refinement. This technique might be helpful in certain instances, but the poor quality of the maps prevents it from being generally useful. Perhaps the tangent refinement technique will be more fruitful in application to neutron protein data when experimental phase information is available.", "PMID": 963578}
{"id": "pubmed23n0032_5611", "title": "A neutron television camera detector.", "content": "The system under development has a large counting rate capability; this is extremely important where the total background count exceeds the total counts in the signals of interest. Its spatial resolution is of the order of one mm, which is perfectly adequate for neutron work, while the screen size of 400 mm is reasonable. The main limitation of the system is its limited counting efficiency, and this is directly attributable to the optical self-absorption of the neutron phosphor. Any newly developed transparent phosphor with the same light output would immediately change the situation. The success of the electronics hardware in reducing random noise is demonstrated in Figure 3, which shows in the bottom trace the live video output when the input to the system is a grey-scale test chart. The top trace is the output after the image has been digitally integrated. Figures 4 and 5 show the monitor outputs of the see articles x-ray system with a \"still\" diffraction pattern of a crystal of GPD (glyceraldehyde-3-phosphate dehydrogenase). Figure 4 is a photograph of the \"live\" video display, and Figure 5 is the digitally summed image. All coherent noise in the system, i.e., all noise synchronized with the TV scans has to be kept lower than the first bit threshold. However, this requirement can be relaxed when dealing with diffraction patterns, such as those from single crystals, for which a local background is subtracted from the pattern.", "contents": "A neutron television camera detector. The system under development has a large counting rate capability; this is extremely important where the total background count exceeds the total counts in the signals of interest. Its spatial resolution is of the order of one mm, which is perfectly adequate for neutron work, while the screen size of 400 mm is reasonable. The main limitation of the system is its limited counting efficiency, and this is directly attributable to the optical self-absorption of the neutron phosphor. Any newly developed transparent phosphor with the same light output would immediately change the situation. The success of the electronics hardware in reducing random noise is demonstrated in Figure 3, which shows in the bottom trace the live video output when the input to the system is a grey-scale test chart. The top trace is the output after the image has been digitally integrated. Figures 4 and 5 show the monitor outputs of the see articles x-ray system with a \"still\" diffraction pattern of a crystal of GPD (glyceraldehyde-3-phosphate dehydrogenase). Figure 4 is a photograph of the \"live\" video display, and Figure 5 is the digitally summed image. All coherent noise in the system, i.e., all noise synchronized with the TV scans has to be kept lower than the first bit threshold. However, this requirement can be relaxed when dealing with diffraction patterns, such as those from single crystals, for which a local background is subtracted from the pattern.", "PMID": 963582}
{"id": "pubmed23n0032_5612", "title": "Fly's eye: a counting camera for thermal neutrons-some applications, problems, and prospects.", "content": "An area detector for thermal neutrons based on image intensification techniques has been described. Some capabilities and limitations of the detection system have been discussed. Among the former are high spatial resolution, high instantaneous counting rate, electronic zoom, time-gating, and integration. The detector is limited in that the maximum counting rate for a resolution element is 60 regularly spaced counts per second. Also, the nonuniformity of response over the detector puts a limit on the useful size and necessitates point-by-point calibration. In addition, a higher efficiency for neutron detection would be desirable. Some typical applications of the system are crystal inspection, neutron magnetic diffraction topography, and searches for temperature-induced changes in diffraction patterns. The future application of solid-state television sensors and microchannel-plate intensifiers to improve the system were briefly mentioned.", "contents": "Fly's eye: a counting camera for thermal neutrons-some applications, problems, and prospects. An area detector for thermal neutrons based on image intensification techniques has been described. Some capabilities and limitations of the detection system have been discussed. Among the former are high spatial resolution, high instantaneous counting rate, electronic zoom, time-gating, and integration. The detector is limited in that the maximum counting rate for a resolution element is 60 regularly spaced counts per second. Also, the nonuniformity of response over the detector puts a limit on the useful size and necessitates point-by-point calibration. In addition, a higher efficiency for neutron detection would be desirable. Some typical applications of the system are crystal inspection, neutron magnetic diffraction topography, and searches for temperature-induced changes in diffraction patterns. The future application of solid-state television sensors and microchannel-plate intensifiers to improve the system were briefly mentioned.", "PMID": 963584}
{"id": "pubmed23n0032_5613", "title": "Investigation of biological macromolecular systems with a pulsed neutron source--a review.", "content": "The conclusion that can be drawn on the basis of the above considerations is that investigation of biological macromolecules and crystalline structures by SAS and diffraction of neutrons with the TOF method is feasible. The main difficulties of the TOF method (the wavelength dependence of the incident beam, resolution power, and detector efficiency; the need for their determination and up-to-date values) are compensated for by its advantages. Both methods allow a high data accumulation rate and optimal employment of the incident neutron spectrum. The latter has been achieved by utilizing a dominant part of the Maxwellian spectrum and by a more uniform distribution of statistical accuracy over the most informative measuring range. Another advantage is the high degree of monochronatization of the incident neutron beam by the TOF method. The rigid requirements concerning the data accumulation rate and the capacity of the on-line system computer memory are technical problems but not basic ones.", "contents": "Investigation of biological macromolecular systems with a pulsed neutron source--a review. The conclusion that can be drawn on the basis of the above considerations is that investigation of biological macromolecules and crystalline structures by SAS and diffraction of neutrons with the TOF method is feasible. The main difficulties of the TOF method (the wavelength dependence of the incident beam, resolution power, and detector efficiency; the need for their determination and up-to-date values) are compensated for by its advantages. Both methods allow a high data accumulation rate and optimal employment of the incident neutron spectrum. The latter has been achieved by utilizing a dominant part of the Maxwellian spectrum and by a more uniform distribution of statistical accuracy over the most informative measuring range. Another advantage is the high degree of monochronatization of the incident neutron beam by the TOF method. The rigid requirements concerning the data accumulation rate and the capacity of the on-line system computer memory are technical problems but not basic ones.", "PMID": 963586}
{"id": "pubmed23n0032_5614", "title": "Some focusing techniques and their application to low-angle neutron scattering.", "content": "Both imaging and simple converging collimator systems can provide a focused neutron beam. Each system has been tested and appears suitable for exploitation. Imaging systems employing bent multilayer monochromators can be made simply by elastically bending a flat multilayer. The periodic spacing of the multilayer can be made to match that of the sample. The converging Soller slit provides a purely geometric means of prouducing a focused beam. Wavelength spread and distribution across the beam can be controlled separately. Soller-slit technology is further advanced than that of multilayers. The experiments sketched above demonstrate that converging or focused neutron beams suitable for low-angle scattering work can be produced with existing technology. Focusing incurs little or no loss of intensity and does not seriously distort the resolution function of a low-angel instrument. It permits a small spectrometer to make use of both advanced detector technology and large samples and thus to approach the capability of a much larger machine.", "contents": "Some focusing techniques and their application to low-angle neutron scattering. Both imaging and simple converging collimator systems can provide a focused neutron beam. Each system has been tested and appears suitable for exploitation. Imaging systems employing bent multilayer monochromators can be made simply by elastically bending a flat multilayer. The periodic spacing of the multilayer can be made to match that of the sample. The converging Soller slit provides a purely geometric means of prouducing a focused beam. Wavelength spread and distribution across the beam can be controlled separately. Soller-slit technology is further advanced than that of multilayers. The experiments sketched above demonstrate that converging or focused neutron beams suitable for low-angle scattering work can be produced with existing technology. Focusing incurs little or no loss of intensity and does not seriously distort the resolution function of a low-angel instrument. It permits a small spectrometer to make use of both advanced detector technology and large samples and thus to approach the capability of a much larger machine.", "PMID": 963588}
{"id": "pubmed23n0032_5615", "title": "The analyzer in neutron protein crystallography.", "content": "The use of a pyrolytic graphite analyzer is shown to contribute to the cleanliness of a neutron protein crystallographic study. If neutrons of approximately 1.5-A wavelength are used, higher orders are reduced by nearly an order of magnitude, and background (arising largely from incoherent inelastic neutron-proton scattering) is reduced by nearly a factor of five. These advantages are gained at the expense of approximately 50% of measured integrated intensity and a distortion of integrated intensity with scattering angle. Because background scatter is generally large compared with peak reflectivity of a protein, the large background reduction by the analyzer more than compensates for reduced peak intensity to improve the statistics of most peak reflectivity measurements. The luminance function distortion of intensity data is not large, produces a slight smearing of atomic scattering density, and can be calculated and adjusted for in the data.", "contents": "The analyzer in neutron protein crystallography. The use of a pyrolytic graphite analyzer is shown to contribute to the cleanliness of a neutron protein crystallographic study. If neutrons of approximately 1.5-A wavelength are used, higher orders are reduced by nearly an order of magnitude, and background (arising largely from incoherent inelastic neutron-proton scattering) is reduced by nearly a factor of five. These advantages are gained at the expense of approximately 50% of measured integrated intensity and a distortion of integrated intensity with scattering angle. Because background scatter is generally large compared with peak reflectivity of a protein, the large background reduction by the analyzer more than compensates for reduced peak intensity to improve the statistics of most peak reflectivity measurements. The luminance function distortion of intensity data is not large, produces a slight smearing of atomic scattering density, and can be calculated and adjusted for in the data.", "PMID": 963589}
{"id": "pubmed23n0032_5616", "title": "Inelastic scattering of neutrons and possible biological applications.", "content": "The field of neutron inelastic scattering has probably been developed to the stage where it can begin to help the biologist. Because essentially no experimental data have been obtained, it is difficult either to draw conclusions or to make forecasts except on the basis of general hypotheses. It seems likely, however, that the next stage is up to biologists. After reviewing those biological problems in which molecular dynamics might play an important role, they should suggest specimens of interest which can give inelastic peaks with existing spectrometers operating with 5 to 10-A neutrons at angles greater than 5degrees and with resolutions of approximately 50 mueV. These specimens may involve molecules slightly smaller and more mobile than some biologists would like, but a successful outcome might lead to the development of spectrometers capable of working in a more satisfactory range. In this event the return may well prove rewarding to the biologists.", "contents": "Inelastic scattering of neutrons and possible biological applications. The field of neutron inelastic scattering has probably been developed to the stage where it can begin to help the biologist. Because essentially no experimental data have been obtained, it is difficult either to draw conclusions or to make forecasts except on the basis of general hypotheses. It seems likely, however, that the next stage is up to biologists. After reviewing those biological problems in which molecular dynamics might play an important role, they should suggest specimens of interest which can give inelastic peaks with existing spectrometers operating with 5 to 10-A neutrons at angles greater than 5degrees and with resolutions of approximately 50 mueV. These specimens may involve molecules slightly smaller and more mobile than some biologists would like, but a successful outcome might lead to the development of spectrometers capable of working in a more satisfactory range. In this event the return may well prove rewarding to the biologists.", "PMID": 963598}
{"id": "pubmed23n0032_5617", "title": "Baculovirus-like particles in the reproductive tracts of female parasitoid wasps.", "content": "Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed the presence of DNA. On the basis of apparent structural homologies, it is suggested that the parasitoid particles are related to baculoviruses.", "contents": "Baculovirus-like particles in the reproductive tracts of female parasitoid wasps. Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed the presence of DNA. On the basis of apparent structural homologies, it is suggested that the parasitoid particles are related to baculoviruses.", "PMID": 963610}
{"id": "pubmed23n0032_5618", "title": "Effect of volatile inhibitors from natural and amended soils on germination of sclerotia of Macrophomina phaseolina.", "content": "Studies on volatile soil fungistasis showed that sclerotia of Macrophomina phaseolina are not sensitive to volatiles liberated by natural soils with varying pH values. Soil amended with alfalfa hay (1%, w/w) liberated volatiles fungistatic to sclerotia only when the assay was performed 2 weeks or less after amendment. Chitin-amended soils liberated fungistatic volatiles from 1 to 3 weeks after amendment. Indirect evidence indicated that ammonia may be the active volatile in amended soil. It was concluded that fungistatic volatiles may not play an important role in the survival of sclerotia in nature.", "contents": "Effect of volatile inhibitors from natural and amended soils on germination of sclerotia of Macrophomina phaseolina. Studies on volatile soil fungistasis showed that sclerotia of Macrophomina phaseolina are not sensitive to volatiles liberated by natural soils with varying pH values. Soil amended with alfalfa hay (1%, w/w) liberated volatiles fungistatic to sclerotia only when the assay was performed 2 weeks or less after amendment. Chitin-amended soils liberated fungistatic volatiles from 1 to 3 weeks after amendment. Indirect evidence indicated that ammonia may be the active volatile in amended soil. It was concluded that fungistatic volatiles may not play an important role in the survival of sclerotia in nature.", "PMID": 963611}
{"id": "pubmed23n0032_5619", "title": "Bacteriocins of Streptococcus bovis.", "content": "Forty-seven strains of Streptococcus bovis were tested for bacteriocin production. Fourteen were found to produce bacteriocins, while all 47 were sensitive to at least one of these bacteriocins. The bacteriocins, on the basis of their host range on S. bovis strains, formed six groups. A representative of each group was selected and characterized by temperature stability, sensitivity to trypsin and lipase, sedimentation by centrifugation, ability to pass through dialysis tubing, host range on other bacterial species, and conditions for production in liquid media. A correlation between mannitol fermentation and bacteriocin production was noted.", "contents": "Bacteriocins of Streptococcus bovis. Forty-seven strains of Streptococcus bovis were tested for bacteriocin production. Fourteen were found to produce bacteriocins, while all 47 were sensitive to at least one of these bacteriocins. The bacteriocins, on the basis of their host range on S. bovis strains, formed six groups. A representative of each group was selected and characterized by temperature stability, sensitivity to trypsin and lipase, sedimentation by centrifugation, ability to pass through dialysis tubing, host range on other bacterial species, and conditions for production in liquid media. A correlation between mannitol fermentation and bacteriocin production was noted.", "PMID": 963612}
{"id": "pubmed23n0032_5620", "title": "Site of action of nonheme iron in the malate (flavine adenine dinucleotide) pathway of Mycobacterium phlei.", "content": "Irradiation with ultraviolet light (360 nm) of cell-free extracts, electron-transport particles, and soluble components from Mycobacterium phlei resulted in the loss of malate oxidation by the flavine adenine dinucleotide pathway both in cell-free extracts and reconstituted systems. Addition of vitamin K1 restored the loss to the extent of 14% and 11% in cell-free extracts and reconstituted systems respectively. Electron-transport particles from M. phlei upon reduction with malate exhibited electron-paramagnetic resonance signals at g = 2.002 and 1.94, characteristic of napthosemiquinone and nonheme iron protein, respectively. Upon irradiating the particles with ultraviolet light (360 nm) these signals were not observed. Particulate flavine-adenine-dinucleotide-dependent malate dehydrogenase (EC 1.1.1.37) of M. phlei assayed by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide and phenazine methosulfate - 2,6-dichlorophenolindophenol systems, which trap electrons at cytochrome c and at the flavine level respectively, was inhibited by o-phenanthroline. These observations suggest that nonheme iron protein is sensitive to ultraviolet light (360 nm) and participates before or in combination with flavine in the malate (flavine adenine dinucleotide) pathway of M. phlei.", "contents": "Site of action of nonheme iron in the malate (flavine adenine dinucleotide) pathway of Mycobacterium phlei. Irradiation with ultraviolet light (360 nm) of cell-free extracts, electron-transport particles, and soluble components from Mycobacterium phlei resulted in the loss of malate oxidation by the flavine adenine dinucleotide pathway both in cell-free extracts and reconstituted systems. Addition of vitamin K1 restored the loss to the extent of 14% and 11% in cell-free extracts and reconstituted systems respectively. Electron-transport particles from M. phlei upon reduction with malate exhibited electron-paramagnetic resonance signals at g = 2.002 and 1.94, characteristic of napthosemiquinone and nonheme iron protein, respectively. Upon irradiating the particles with ultraviolet light (360 nm) these signals were not observed. Particulate flavine-adenine-dinucleotide-dependent malate dehydrogenase (EC 1.1.1.37) of M. phlei assayed by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide and phenazine methosulfate - 2,6-dichlorophenolindophenol systems, which trap electrons at cytochrome c and at the flavine level respectively, was inhibited by o-phenanthroline. These observations suggest that nonheme iron protein is sensitive to ultraviolet light (360 nm) and participates before or in combination with flavine in the malate (flavine adenine dinucleotide) pathway of M. phlei.", "PMID": 963613}
{"id": "pubmed23n0032_5621", "title": "Differences in physicochemical and antigenic properties of chlamydial strains.", "content": "Antigenic analysis of Chlamydia psittaci, C. trachomatis, and Lymphogranuloma venereum (LGV) revealed basic physicochemical differences among the three chlamydial strains. These were manifested in structural, isoelectric points, absorption spectra, and in the characteristics of the chromophobe-containing proteins. The effective period of sonication for C. psittaci and C. trachomatis is around 60 min during which the linkages most susceptible to external sonication forces were broken, releasing all attainable cellular components. Denaturation studies demonstrated that less than 50% of protein content of C. psittaci was denatured after 1 h of sonication, only 5% in the case of C. trachomatis. The protein and carbohydrate content of the most reactive fractions in macrophage-spreading inhibition test were different for LGV and C. trachomatis. The structural differences appear to determine the antigenic properties observed among the chlamydial strains as well as the specificity and probably the mechanisms (s) of cellular immune reactivity to Chlamydiae. This in turn may explain the failure of chlamydial vaccines, prepared from stock strains, to protect immunized children against 'wild' chlamydial strains.", "contents": "Differences in physicochemical and antigenic properties of chlamydial strains. Antigenic analysis of Chlamydia psittaci, C. trachomatis, and Lymphogranuloma venereum (LGV) revealed basic physicochemical differences among the three chlamydial strains. These were manifested in structural, isoelectric points, absorption spectra, and in the characteristics of the chromophobe-containing proteins. The effective period of sonication for C. psittaci and C. trachomatis is around 60 min during which the linkages most susceptible to external sonication forces were broken, releasing all attainable cellular components. Denaturation studies demonstrated that less than 50% of protein content of C. psittaci was denatured after 1 h of sonication, only 5% in the case of C. trachomatis. The protein and carbohydrate content of the most reactive fractions in macrophage-spreading inhibition test were different for LGV and C. trachomatis. The structural differences appear to determine the antigenic properties observed among the chlamydial strains as well as the specificity and probably the mechanisms (s) of cellular immune reactivity to Chlamydiae. This in turn may explain the failure of chlamydial vaccines, prepared from stock strains, to protect immunized children against 'wild' chlamydial strains.", "PMID": 963615}
{"id": "pubmed23n0032_5622", "title": "Colonial variation in Xanthomonas campestris NRRL B-1459 and characterization of the polysaccharide from a variant strain.", "content": "Stock cultures of Xanthomonas campestris NRRL B-1459 require special attention to maintenance and propagation to assure consistent production in good yields of the extracellular polysaccharide xanthan. Under customary conditions of propagative maintenance on agar slants, variant colony types develop that are smaller in size than the normal type. The rate of regression of the normal to the variant forms was diminished when the D-glucose content of the stock medium was sufficient to avoid depletion during storage and when transfer to fresh medium was reduced to 14-day intervals. Under conditions for polysaccharide production, the normal large-colony type gives crude culture liquors after 48 h of 7000 centipoise (cp) viscosity; the predominant variant form gives only 4000 cp. On the basis of 2.1% initial D-glucose, biopolymer yields for the normal and variant strains were 62 and 43%, respectively. Polysaccharide produced by the variant (small-colony type) differs adversely in solution properties from that of the parent strain (large-colony type); it differs also in its lower content of pyruvic acid and O-acetyl substituents. The molar ratios of constituent sugars (D-glucose, D-mannose, and D-glucuronic acid), however, were identical in polysaccharides with the normal and variant strains. Exclusion of colonial variants from fermentations is prerequisite to production of xanthan optimum in properties and yield.", "contents": "Colonial variation in Xanthomonas campestris NRRL B-1459 and characterization of the polysaccharide from a variant strain. Stock cultures of Xanthomonas campestris NRRL B-1459 require special attention to maintenance and propagation to assure consistent production in good yields of the extracellular polysaccharide xanthan. Under customary conditions of propagative maintenance on agar slants, variant colony types develop that are smaller in size than the normal type. The rate of regression of the normal to the variant forms was diminished when the D-glucose content of the stock medium was sufficient to avoid depletion during storage and when transfer to fresh medium was reduced to 14-day intervals. Under conditions for polysaccharide production, the normal large-colony type gives crude culture liquors after 48 h of 7000 centipoise (cp) viscosity; the predominant variant form gives only 4000 cp. On the basis of 2.1% initial D-glucose, biopolymer yields for the normal and variant strains were 62 and 43%, respectively. Polysaccharide produced by the variant (small-colony type) differs adversely in solution properties from that of the parent strain (large-colony type); it differs also in its lower content of pyruvic acid and O-acetyl substituents. The molar ratios of constituent sugars (D-glucose, D-mannose, and D-glucuronic acid), however, were identical in polysaccharides with the normal and variant strains. Exclusion of colonial variants from fermentations is prerequisite to production of xanthan optimum in properties and yield.", "PMID": 963616}
{"id": "pubmed23n0032_5623", "title": "Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levels.", "content": "The reduction of acetylene to ethylene by stationary (non-shaking) cultures of free-living rhizobia under atmospheric oxygen levels has been demonstrated. Under these conditions the development of the activity is inhibited by 10 mM NH4Cl and about 20% of oxygen is required for maximal activity. When the stationary cultures were shaken, oxygen concentrations of 1% and higher were found to be inhibitory. Specific activities of 20 and 40 nmol of acetylene reduced h-1 mg-1 protein were observed.", "contents": "Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levels. The reduction of acetylene to ethylene by stationary (non-shaking) cultures of free-living rhizobia under atmospheric oxygen levels has been demonstrated. Under these conditions the development of the activity is inhibited by 10 mM NH4Cl and about 20% of oxygen is required for maximal activity. When the stationary cultures were shaken, oxygen concentrations of 1% and higher were found to be inhibitory. Specific activities of 20 and 40 nmol of acetylene reduced h-1 mg-1 protein were observed.", "PMID": 963617}
{"id": "pubmed23n0032_5624", "title": "In field survival of Rhizoctonia solani in soil and in diseased sugarbeets.", "content": "Persistence of Rhizotonia solani in the field was investigated by ascertaining survival (competitive saprophytic activity) in soil and survival in diseased plants. Except for one instance, low levels of R. solani survived overwinter in artificially and naturally infested soils. In a sandy loam soil, cropped to sugarbeets, inoculum density increased throughout the growing season from low early spring levels to high levels in July and August. In a silty clay soil, cropped to sugarbeets, inoculum density remained low with only a slight increase throughout the growing season. Survival of R. solani in diseased sugarbeets placed on the soil surface was greater than survival in diseased beets buried in soil. Little reduction in percentages of beets yeilding R. SOLANI COLONIES TOOK PLACE FROM November to April in either buried or unburied beets. The major reduction in survival of R. solani in buried beets occurred during the 6-week interval from April to June.", "contents": "In field survival of Rhizoctonia solani in soil and in diseased sugarbeets. Persistence of Rhizotonia solani in the field was investigated by ascertaining survival (competitive saprophytic activity) in soil and survival in diseased plants. Except for one instance, low levels of R. solani survived overwinter in artificially and naturally infested soils. In a sandy loam soil, cropped to sugarbeets, inoculum density increased throughout the growing season from low early spring levels to high levels in July and August. In a silty clay soil, cropped to sugarbeets, inoculum density remained low with only a slight increase throughout the growing season. Survival of R. solani in diseased sugarbeets placed on the soil surface was greater than survival in diseased beets buried in soil. Little reduction in percentages of beets yeilding R. SOLANI COLONIES TOOK PLACE FROM November to April in either buried or unburied beets. The major reduction in survival of R. solani in buried beets occurred during the 6-week interval from April to June.", "PMID": 963618}
{"id": "pubmed23n0032_5625", "title": "A modified procedure for studying enzyme secretion in yeast sphaeroplasts: subcellular distribution of invertase.", "content": "A significantly modified procedure for investigating enzyme secretion from yeast sphaeroplasts, and results from its application are described. Sphaeroplasts were derepressed for invertase biosynthesis in the presence of helicase and fractionated to reveal the distribution of high and low molecular weight forms of invertase. Secreted enzyme was found to be of high molecular weight, exclusively. Less than 10% of the total invertase activity was present in washed sphaeroplasts and of this, 43% was soluble, consisting of both high and low molecular weight forms of invertase. Washed membranes retained 32% of the internal invertase activity, and on solubilization with Triton X-100 the enzyme was found to be of an intermediate molecular weight. These results are consistent with the hypothesis that invertase is glycosylated at the plasma membrane.", "contents": "A modified procedure for studying enzyme secretion in yeast sphaeroplasts: subcellular distribution of invertase. A significantly modified procedure for investigating enzyme secretion from yeast sphaeroplasts, and results from its application are described. Sphaeroplasts were derepressed for invertase biosynthesis in the presence of helicase and fractionated to reveal the distribution of high and low molecular weight forms of invertase. Secreted enzyme was found to be of high molecular weight, exclusively. Less than 10% of the total invertase activity was present in washed sphaeroplasts and of this, 43% was soluble, consisting of both high and low molecular weight forms of invertase. Washed membranes retained 32% of the internal invertase activity, and on solubilization with Triton X-100 the enzyme was found to be of an intermediate molecular weight. These results are consistent with the hypothesis that invertase is glycosylated at the plasma membrane.", "PMID": 963619}
{"id": "pubmed23n0032_5626", "title": "[Metabolic activity of Candida lipolytica adsorbed to bentonite with hydrophobic chains].", "content": "The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stimulate growth, but assimilation of n-tetradecane is enhanced with certain concentrations of the adsorbant. On glucose, addition of hydrophobic chains on the adsorbant does not change the effects of bentonite on exogenous respiration and substrate assimilation.", "contents": "[Metabolic activity of Candida lipolytica adsorbed to bentonite with hydrophobic chains]. The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stimulate growth, but assimilation of n-tetradecane is enhanced with certain concentrations of the adsorbant. On glucose, addition of hydrophobic chains on the adsorbant does not change the effects of bentonite on exogenous respiration and substrate assimilation.", "PMID": 963620}
{"id": "pubmed23n0032_5627", "title": "Characterization of group A streptococcal M-proteins purified by two methods.", "content": "Ten different group A streptococcal M-protein preparations purified by trichloroacetic acid precipitation and three M-protein preparations purified by cellulose chromatography were examined by SDS and polyacrylamide gel electrophoresis, and analyzed for amino acid composition and N-terminal amino acids. Fingerprinting (both tryptic and chymotryptic) was performed on the cellulose purified preparations of M1, M12, and M29 proteins which showed these proteins to be structurally related. Trypsin produced mas with 37 to 42 peptides, whereas chymotrypsin digestion resulted in 8 to 12 peptides,  depending on the M-type. Sequencing was performed on the M12 protein and tentative identification of nine N-terminal amino acids made. Molecular weights of the cellulose and TCA-purified M-proteins were determined by SDS gel electrophoresis and chromatography on G-200 Sephadex, with comparable results, indicating followed the patterns established for M-proteins, with high concentrations of lysine, aspartic acid, glutamic acid, alanine, and leucine. All 10 proteins had L-alanine as their N-terminal amino acid. Evidence for a one way cross-reaction between type 1 and type 29 streptococci was also found.", "contents": "Characterization of group A streptococcal M-proteins purified by two methods. Ten different group A streptococcal M-protein preparations purified by trichloroacetic acid precipitation and three M-protein preparations purified by cellulose chromatography were examined by SDS and polyacrylamide gel electrophoresis, and analyzed for amino acid composition and N-terminal amino acids. Fingerprinting (both tryptic and chymotryptic) was performed on the cellulose purified preparations of M1, M12, and M29 proteins which showed these proteins to be structurally related. Trypsin produced mas with 37 to 42 peptides, whereas chymotrypsin digestion resulted in 8 to 12 peptides,  depending on the M-type. Sequencing was performed on the M12 protein and tentative identification of nine N-terminal amino acids made. Molecular weights of the cellulose and TCA-purified M-proteins were determined by SDS gel electrophoresis and chromatography on G-200 Sephadex, with comparable results, indicating followed the patterns established for M-proteins, with high concentrations of lysine, aspartic acid, glutamic acid, alanine, and leucine. All 10 proteins had L-alanine as their N-terminal amino acid. Evidence for a one way cross-reaction between type 1 and type 29 streptococci was also found.", "PMID": 963621}
{"id": "pubmed23n0032_5628", "title": "The citric acid fermentation by Aspergillus niger: regulation by zinc of growth and acidogenesis.", "content": "The citric acid fermentation by Aspergillus niger is divided into two consecutive phases, a growth phase when the cells proliferate but do not accumulate citrate, followed by an accumulating phase when they produce citrate but do not proliferate, or else do so at a much reduced rate. When studied in a low sucrose (0.4-0.8%) mpinimal salts medium the growth-accumulation alternative was controlled by the concentration of zinc: high zinc (about 1-2 muM) maintained growth phase, while at low zinc (below 1 muM) growth became limited by zinc deficiency and the cultures passed into accumulating phase. Addition of zinc to accumulating cultures resulted in their reversion to growth phase. Iron, manganese, calcium at concentrations as high as 5-10 muM had no influence on either growth or citrate accumulation. These results suggest that zinc plays a role in the regulation of growth and citric acid accumulation.", "contents": "The citric acid fermentation by Aspergillus niger: regulation by zinc of growth and acidogenesis. The citric acid fermentation by Aspergillus niger is divided into two consecutive phases, a growth phase when the cells proliferate but do not accumulate citrate, followed by an accumulating phase when they produce citrate but do not proliferate, or else do so at a much reduced rate. When studied in a low sucrose (0.4-0.8%) mpinimal salts medium the growth-accumulation alternative was controlled by the concentration of zinc: high zinc (about 1-2 muM) maintained growth phase, while at low zinc (below 1 muM) growth became limited by zinc deficiency and the cultures passed into accumulating phase. Addition of zinc to accumulating cultures resulted in their reversion to growth phase. Iron, manganese, calcium at concentrations as high as 5-10 muM had no influence on either growth or citrate accumulation. These results suggest that zinc plays a role in the regulation of growth and citric acid accumulation.", "PMID": 963622}
{"id": "pubmed23n0032_5629", "title": "The fine structure of conidial development in the genus Torula. IV. T. thermophila Cooney & Emerson.", "content": "Torula thermophila produced typical chlamydospores either as intercalary chains within prostrate hyphae or as terminal swellings on short, lateral, hyphal branches. Mature chlamydo-spores were spherical, dark brown, smooth-surfaced structured structures with thick, single-layed cell walls (= secondary wall layer) usually differentiated into an outer electron-dense zone and an inner electron-transparent zone. Disarticulation and sport release occurred after the disintegration of the original hyphal wall. The thallospores of T. thermophila arise in a manner different from the blastospores produced by other species of Torula and are structurally more closely related to the spores produced by Humicola insolens. However until further work has been completed on spore development in the Torula-Humicola complex of fungi the name T. thermophila is retained.", "contents": "The fine structure of conidial development in the genus Torula. IV. T. thermophila Cooney & Emerson. Torula thermophila produced typical chlamydospores either as intercalary chains within prostrate hyphae or as terminal swellings on short, lateral, hyphal branches. Mature chlamydo-spores were spherical, dark brown, smooth-surfaced structured structures with thick, single-layed cell walls (= secondary wall layer) usually differentiated into an outer electron-dense zone and an inner electron-transparent zone. Disarticulation and sport release occurred after the disintegration of the original hyphal wall. The thallospores of T. thermophila arise in a manner different from the blastospores produced by other species of Torula and are structurally more closely related to the spores produced by Humicola insolens. However until further work has been completed on spore development in the Torula-Humicola complex of fungi the name T. thermophila is retained.", "PMID": 963623}
{"id": "pubmed23n0032_5630", "title": "Antibody production in milk serum of goats experimentally infected with Neisseria gonorrhoeae.", "content": "Instillation of the goat's mammary gland with Neisseria gonorrhoeae colony type T1 has elicited and antibody response in the goat milk serum (GMS). Purification, and characterization of the GMS by gel filtration, electrophoresis, immunodiffusion, analytical ultracentrifugation, and serological analyses demonstrated thtat the active immune component was mainly in the IgA and IgG fractions (F2 and F3) of GMS.", "contents": "Antibody production in milk serum of goats experimentally infected with Neisseria gonorrhoeae. Instillation of the goat's mammary gland with Neisseria gonorrhoeae colony type T1 has elicited and antibody response in the goat milk serum (GMS). Purification, and characterization of the GMS by gel filtration, electrophoresis, immunodiffusion, analytical ultracentrifugation, and serological analyses demonstrated thtat the active immune component was mainly in the IgA and IgG fractions (F2 and F3) of GMS.", "PMID": 963624}
{"id": "pubmed23n0032_5631", "title": "California encephalitis virus proliferation in Yukon mosquitoes incubated at low temperatures.", "content": "Replication of a subarctic Bunyavirus, California encephalitis (snowshoe hare subtype), was detected in salivary glands and thoraces of wild-caught Aedes communis mosquitoes from the Yokon Territory, after intrathoracic inoculation with 0.1 to 100 mouse LD50 virus, and incubation for 7 to 21 days throughout their viable temperature range of 0 to 23 degrees C. Immunoperoxidase staining confirmed that viral replication occurred in the cytoplasm of acinar cells of salivary glands, both by ligh microscopy and electron microscopy. Replication of another subarctic Bunyavirus, Northway, and a subtropical Flavivirus, Murray Valley encephalitis, was also demonstrated by infectivity by infectivity titrations and immun operoxidase reactions in salivary glands of A. communis incubated at 0, 13, and 23 degrees C for 7 to 21 days.", "contents": "California encephalitis virus proliferation in Yukon mosquitoes incubated at low temperatures. Replication of a subarctic Bunyavirus, California encephalitis (snowshoe hare subtype), was detected in salivary glands and thoraces of wild-caught Aedes communis mosquitoes from the Yokon Territory, after intrathoracic inoculation with 0.1 to 100 mouse LD50 virus, and incubation for 7 to 21 days throughout their viable temperature range of 0 to 23 degrees C. Immunoperoxidase staining confirmed that viral replication occurred in the cytoplasm of acinar cells of salivary glands, both by ligh microscopy and electron microscopy. Replication of another subarctic Bunyavirus, Northway, and a subtropical Flavivirus, Murray Valley encephalitis, was also demonstrated by infectivity by infectivity titrations and immun operoxidase reactions in salivary glands of A. communis incubated at 0, 13, and 23 degrees C for 7 to 21 days.", "PMID": 963625}
{"id": "pubmed23n0032_5632", "title": "Toxicity and plant growth regulator effects of cytochalasin H isolated from Phomopsis sp.", "content": "A biologically active metabolite was found in crude extracts of an unidentified species of Phomopsis isolated from weevil-damaged pecans. Purified metabolite was toxic to day-old cockerels, with an LD50 of 12.5 mg/kg, and was markedly inhibitory to growth the floral development of tobacco plants at concentrations of 10(-2) to 10(-4) M. IR and 1H-nmr spectra indicated an alkylpheny moiety with a single nitrogen atom present as a secondary amide; and acetate ester, OH, CH3, and a trans-double bond function. Elemental and high-resolution mass-spectral analysis indicated a formula of C30H39NO5 and a molecular weight of 493.2886. The metabolite was identified as cytochalasin H.", "contents": "Toxicity and plant growth regulator effects of cytochalasin H isolated from Phomopsis sp. A biologically active metabolite was found in crude extracts of an unidentified species of Phomopsis isolated from weevil-damaged pecans. Purified metabolite was toxic to day-old cockerels, with an LD50 of 12.5 mg/kg, and was markedly inhibitory to growth the floral development of tobacco plants at concentrations of 10(-2) to 10(-4) M. IR and 1H-nmr spectra indicated an alkylpheny moiety with a single nitrogen atom present as a secondary amide; and acetate ester, OH, CH3, and a trans-double bond function. Elemental and high-resolution mass-spectral analysis indicated a formula of C30H39NO5 and a molecular weight of 493.2886. The metabolite was identified as cytochalasin H.", "PMID": 963626}
{"id": "pubmed23n0032_5633", "title": "A cellulase complex in culture filtrates of Penicillium citrinum.", "content": "During growth in a liquid medium that contained a single soluble or an insoluble cellulosic carbon source Penicillium citrinum released a complex of cellulase enzymes into the medium. A temperature of 30 degrees C was best for cellulase production. Presence of carbon-containing compounds, particularly glucose, inhibited cellulase activity. The enzyme complex was separated by gel filtration followed by ion-exchange chromatography into 11 components, 4 of high molecular weight and 7 of low molecular weight. One of the components (Bb) had the character of C1 cellulase enzyme. When the components were combined they released more reducing sugars from cullulosic substrates than when they were used singly.", "contents": "A cellulase complex in culture filtrates of Penicillium citrinum. During growth in a liquid medium that contained a single soluble or an insoluble cellulosic carbon source Penicillium citrinum released a complex of cellulase enzymes into the medium. A temperature of 30 degrees C was best for cellulase production. Presence of carbon-containing compounds, particularly glucose, inhibited cellulase activity. The enzyme complex was separated by gel filtration followed by ion-exchange chromatography into 11 components, 4 of high molecular weight and 7 of low molecular weight. One of the components (Bb) had the character of C1 cellulase enzyme. When the components were combined they released more reducing sugars from cullulosic substrates than when they were used singly.", "PMID": 963627}
{"id": "pubmed23n0032_5634", "title": "In vitro binding of sterols by extracts from Mucor rouxii cells grown under different environmental conditions.", "content": "Aqueous cell wall and cytoplasm extracts from Mucor rouxii cells grown under acrobic and anaerobic (oxygen-limiting) conditions were obtained. Both of the extracts from the anaerobic and aerobic cells solubilized lanosterol and ergosterol but not to the same extent; ergosterol was complexed about 2-3 times greater than the same concentration of lanosterol. It was also apparent that the relative bindingcapacities of the cell extracts were dependent on the cell growth environment. Two additional fractions were obtained from each extract by (a) methanol precipitation and (b) refluxing in 2% aqueous potassium hydroxide solution followed by methanol precipitation. The binding activities of these precipitates and the corresponding precipitates from yeast extract were compared and the results indicated a range of binding activites which depended on both the extract and the sterol. The data suggested that the in vitro sterol binding of M. rouxii extracts was not due to one particular macromolecule but that a number of compounds were involved in this complexation process.", "contents": "In vitro binding of sterols by extracts from Mucor rouxii cells grown under different environmental conditions. Aqueous cell wall and cytoplasm extracts from Mucor rouxii cells grown under acrobic and anaerobic (oxygen-limiting) conditions were obtained. Both of the extracts from the anaerobic and aerobic cells solubilized lanosterol and ergosterol but not to the same extent; ergosterol was complexed about 2-3 times greater than the same concentration of lanosterol. It was also apparent that the relative bindingcapacities of the cell extracts were dependent on the cell growth environment. Two additional fractions were obtained from each extract by (a) methanol precipitation and (b) refluxing in 2% aqueous potassium hydroxide solution followed by methanol precipitation. The binding activities of these precipitates and the corresponding precipitates from yeast extract were compared and the results indicated a range of binding activites which depended on both the extract and the sterol. The data suggested that the in vitro sterol binding of M. rouxii extracts was not due to one particular macromolecule but that a number of compounds were involved in this complexation process.", "PMID": 963628}
{"id": "pubmed23n0032_5635", "title": "Photoreactivating property of the Vibrio cholerae cell system.", "content": "Ultraviolet-inactivated chlora phage PL163/10 underwent a maximum of abot 36% of photoreactivation within the host V. cholerae cells. UV-inactivated but non-infected V. cholerae cells also underwent the same degree of photoreactivation.", "contents": "Photoreactivating property of the Vibrio cholerae cell system. Ultraviolet-inactivated chlora phage PL163/10 underwent a maximum of abot 36% of photoreactivation within the host V. cholerae cells. UV-inactivated but non-infected V. cholerae cells also underwent the same degree of photoreactivation.", "PMID": 963629}
{"id": "pubmed23n0032_5636", "title": "The metabolism of carbohydrates by extremely halophilic bacteria: identification of galactonic acid as a product of galactose metabolism.", "content": "Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged to be galactonic acid, based on the behavior of the acetylmethyl ester derivative of the product and the pentaacetyl derivative of the galactonic methyl ester during gas chromatography.", "contents": "The metabolism of carbohydrates by extremely halophilic bacteria: identification of galactonic acid as a product of galactose metabolism. Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged to be galactonic acid, based on the behavior of the acetylmethyl ester derivative of the product and the pentaacetyl derivative of the galactonic methyl ester during gas chromatography.", "PMID": 963630}
{"id": "pubmed23n0032_5637", "title": "Magnesium requirement of Staphylococcus aureus for repair from sublethal heat injury.", "content": "Heating in potassium phosphate buffer causes Staphylococcus aureus to lose its salt tolerance and 30-40% of its cellular Mg2+. Repair from injury (regain of salt tolerance) occurred when injured cells were incubated under optimal conditions in synthetic media containing penicillin to prevent growth. Cells died when phosphates or amin acids were omitted from the medium. Omission of vitamins, glucose, Na+, and K+ had no effect. Omission of Mg2+ diminished repair. In a minimal repair medium (MRM) which contained only 3 X 10(-6) M Mg (as an impurity), injured cells rapidly regained their original Mg content. About 20-50% of the cells also regained their salt tolerance provided that less than 10(9) cells/ml were used. With 10(10) cells/ml there was no repair and cellular Mg content was half that of the control. Addition of 10(-3) M ethylenediamine-tetraacetic acid (EDTA) to MRM also prevented repair. Addition of 10(-2) M Mg to MRM EDTA permitted complete repair.", "contents": "Magnesium requirement of Staphylococcus aureus for repair from sublethal heat injury. Heating in potassium phosphate buffer causes Staphylococcus aureus to lose its salt tolerance and 30-40% of its cellular Mg2+. Repair from injury (regain of salt tolerance) occurred when injured cells were incubated under optimal conditions in synthetic media containing penicillin to prevent growth. Cells died when phosphates or amin acids were omitted from the medium. Omission of vitamins, glucose, Na+, and K+ had no effect. Omission of Mg2+ diminished repair. In a minimal repair medium (MRM) which contained only 3 X 10(-6) M Mg (as an impurity), injured cells rapidly regained their original Mg content. About 20-50% of the cells also regained their salt tolerance provided that less than 10(9) cells/ml were used. With 10(10) cells/ml there was no repair and cellular Mg content was half that of the control. Addition of 10(-3) M ethylenediamine-tetraacetic acid (EDTA) to MRM also prevented repair. Addition of 10(-2) M Mg to MRM EDTA permitted complete repair.", "PMID": 963632}
{"id": "pubmed23n0032_5638", "title": "The relationship between chemical structure of attractants and chemotaxis by a marine bacterium.", "content": "The chemotactic responses of a marine pseudomonad to steroisomers and analogues of amino acids and sugars were tested. The data reveal that the bacterium is equally attracted to D, L, and DL forms of the amino acids. In contrastr, chemical analogues of the amino acids and glucose yielded significantly lower chemotactic responses. The threshold of bacterial detection was 10(-8) M for leucine and cysteine. However, the threshold molarity of most of the analogues was higher than those of the related amino acids and sugars.", "contents": "The relationship between chemical structure of attractants and chemotaxis by a marine bacterium. The chemotactic responses of a marine pseudomonad to steroisomers and analogues of amino acids and sugars were tested. The data reveal that the bacterium is equally attracted to D, L, and DL forms of the amino acids. In contrastr, chemical analogues of the amino acids and glucose yielded significantly lower chemotactic responses. The threshold of bacterial detection was 10(-8) M for leucine and cysteine. However, the threshold molarity of most of the analogues was higher than those of the related amino acids and sugars.", "PMID": 963633}
{"id": "pubmed23n0032_5639", "title": "Biodegradation rates of components of petroleum.", "content": "Rates of microbial biodegradation of components of South Louisiana crude oil were determined by computer analysis of data obtained from computerized mass spectrometry. Total residue of the oil decreased exponentially with time, with maximum decrease noted at logarithmic phase, whereas asphaltenes and resins increased at the logarithmic phase of growth. Saturates decreased continuously during growth. Microbial degradation of components of crude oil was concluded to be a dynamic process.", "contents": "Biodegradation rates of components of petroleum. Rates of microbial biodegradation of components of South Louisiana crude oil were determined by computer analysis of data obtained from computerized mass spectrometry. Total residue of the oil decreased exponentially with time, with maximum decrease noted at logarithmic phase, whereas asphaltenes and resins increased at the logarithmic phase of growth. Saturates decreased continuously during growth. Microbial degradation of components of crude oil was concluded to be a dynamic process.", "PMID": 963634}
{"id": "pubmed23n0032_5640", "title": "An unusual occurrence of multiple sclerosis in a small rural community.", "content": "Ten patients with multiple sclerosis were found to have lived in close proximity in a Nova Scotia farming community of 150 people. All had drunk unpasteurized milk as children, were teetotallers, ate a high animal fat diet, and were well educated. Of greater interest was the observation that six of the ten cases were related in two family groups. The only time all patients lived in the community at the same time was in 1951 and 1952 during a polio outbreak. The relationship of polio to multiple sclerosis bears further study. The average age of the patients when they had measles was 11.8 years. Evidence suggests a link between risk of multiple sclerosis and both late onset of measles and pubertal age. Late onset of measles may be important in this cluster. Further epidemiological studies are needed to examine the age of onset of measles in M.S. cases.", "contents": "An unusual occurrence of multiple sclerosis in a small rural community. Ten patients with multiple sclerosis were found to have lived in close proximity in a Nova Scotia farming community of 150 people. All had drunk unpasteurized milk as children, were teetotallers, ate a high animal fat diet, and were well educated. Of greater interest was the observation that six of the ten cases were related in two family groups. The only time all patients lived in the community at the same time was in 1951 and 1952 during a polio outbreak. The relationship of polio to multiple sclerosis bears further study. The average age of the patients when they had measles was 11.8 years. Evidence suggests a link between risk of multiple sclerosis and both late onset of measles and pubertal age. Late onset of measles may be important in this cluster. Further epidemiological studies are needed to examine the age of onset of measles in M.S. cases.", "PMID": 963635}
{"id": "pubmed23n0032_5641", "title": "Shy-Drager syndrome. Neuropathological correlation and response to levodopa therapy.", "content": "The post-mortem examination of the nervous system of a patient with Shy-Drager syndrome successfully treated with levodopa (Sharpe et al, 1972) revealed features of striato-nigral degeneration and amyotrophic lateral sclerosis, a cerebellar system degeneration and a loss of approximately 75% of sympathetic preganglionic neurons. Lewy bodies were not present and no detectable changes were observed in the sympathetic prevertebral ganglia. While the limited and transient beneficial effect of levodopa on the bradykinesia in our case is possibly due to the progressive loss of striatal dopaminergic receptors seen in striatonigral degeneration, we propose that in Shy-Drager syndrome, levodopa therapy benefits orthostatic hypotension because of a suppression of the central depressor action of this drug. This suppression is attributable to functional disconnection of sympathetic ganglia secondary to the loss of preganglionic neurons or to degeneration of central autonomic catecholaminergic systems.", "contents": "Shy-Drager syndrome. Neuropathological correlation and response to levodopa therapy. The post-mortem examination of the nervous system of a patient with Shy-Drager syndrome successfully treated with levodopa (Sharpe et al, 1972) revealed features of striato-nigral degeneration and amyotrophic lateral sclerosis, a cerebellar system degeneration and a loss of approximately 75% of sympathetic preganglionic neurons. Lewy bodies were not present and no detectable changes were observed in the sympathetic prevertebral ganglia. While the limited and transient beneficial effect of levodopa on the bradykinesia in our case is possibly due to the progressive loss of striatal dopaminergic receptors seen in striatonigral degeneration, we propose that in Shy-Drager syndrome, levodopa therapy benefits orthostatic hypotension because of a suppression of the central depressor action of this drug. This suppression is attributable to functional disconnection of sympathetic ganglia secondary to the loss of preganglionic neurons or to degeneration of central autonomic catecholaminergic systems.", "PMID": 963636}
{"id": "pubmed23n0032_5642", "title": "Intellectual performance in multi-infarct dementia and Alzheimer's disease a replication study.", "content": "A consistent feature in dementia is an overall intellectual deterioration. The present study investigated the intellectual performance of patients with Alzeheimer's disease (AD) and multi-infarct dementia (MID) using the Wechsler Adult Intelligence Scale (WAIS). For reliability and generality purposes two independent samples of patients were collected. Significant differences in Education Level (EDU) and Performance IQ (PIQ) were obtained for the first sample, with the AD group having a significantly higher EDU level. The MID group obtained a higher PIQ. No significant differences were found in the second sample, but EDU level approached significance with the AD group again having a higher EDU level. A discriminant function analysis classified 81% of the patients in the first sample and 100% diagnostic accuracy was obtained for the second sample using the 11 predictor variables. A maximum R2 stepwise regression was performed in order to detect the \"best\" model of variables discriminating between the diagnostic groups. For the first sample the \"best\" model was the two variable model, including EDU and Full Scale IQ, accounting for 40% of the variance. The simplest model for the second sample was the one variable model including EDU, accounting for 20% of the variance. Quantitative differences were found between the AD samples. Time since onset of the disease was offered as a possible influence in the quantitative differences in the AD samples. Sampling biases in the behavioral study of dementia are discussed. Successful replication was obtained.", "contents": "Intellectual performance in multi-infarct dementia and Alzheimer's disease a replication study. A consistent feature in dementia is an overall intellectual deterioration. The present study investigated the intellectual performance of patients with Alzeheimer's disease (AD) and multi-infarct dementia (MID) using the Wechsler Adult Intelligence Scale (WAIS). For reliability and generality purposes two independent samples of patients were collected. Significant differences in Education Level (EDU) and Performance IQ (PIQ) were obtained for the first sample, with the AD group having a significantly higher EDU level. The MID group obtained a higher PIQ. No significant differences were found in the second sample, but EDU level approached significance with the AD group again having a higher EDU level. A discriminant function analysis classified 81% of the patients in the first sample and 100% diagnostic accuracy was obtained for the second sample using the 11 predictor variables. A maximum R2 stepwise regression was performed in order to detect the \"best\" model of variables discriminating between the diagnostic groups. For the first sample the \"best\" model was the two variable model, including EDU and Full Scale IQ, accounting for 40% of the variance. The simplest model for the second sample was the one variable model including EDU, accounting for 20% of the variance. Quantitative differences were found between the AD samples. Time since onset of the disease was offered as a possible influence in the quantitative differences in the AD samples. Sampling biases in the behavioral study of dementia are discussed. Successful replication was obtained.", "PMID": 963637}
{"id": "pubmed23n0032_5643", "title": "Intracranial dysgerminomas.", "content": "We have reported three cases of intracranial dysgerminoma. The origin of these tumors has been controversial for a long time. It is now accepted that they develop from germ cells. The diagnosis is often difficult to confirm because of the varity of signs. If their origin is now accepted, the best way to deal with these lesions is still controversial. They can be macroscopically removed if they are in the pineal region. Such treatment is not possible for suprasellar tumors. Since these tumors can metastasize, radiation should be administered as soon as a pathological diagnosis is obtained. It is our belief that it should be administered to the whole spinal axis.", "contents": "Intracranial dysgerminomas. We have reported three cases of intracranial dysgerminoma. The origin of these tumors has been controversial for a long time. It is now accepted that they develop from germ cells. The diagnosis is often difficult to confirm because of the varity of signs. If their origin is now accepted, the best way to deal with these lesions is still controversial. They can be macroscopically removed if they are in the pineal region. Such treatment is not possible for suprasellar tumors. Since these tumors can metastasize, radiation should be administered as soon as a pathological diagnosis is obtained. It is our belief that it should be administered to the whole spinal axis.", "PMID": 963638}
{"id": "pubmed23n0032_5644", "title": "Congenital intraspinal extradural cysts (intraspinal meningocele).", "content": "A case of congenital, intraspinal, extradural cyst is reported with pain as the only presenting feature. In the past, the lack of pain has been considered one of the characteristics of these rare lesions. In other reported cases, when pain has been present, it was minimal and never the presenting feature. The clinical and radiographic features and the surgical treatment are described. The pathogenesis is discussed and the literature reviewed. An addition to the surgical technique is given whereby one of the post operative complications may be obviated.", "contents": "Congenital intraspinal extradural cysts (intraspinal meningocele). A case of congenital, intraspinal, extradural cyst is reported with pain as the only presenting feature. In the past, the lack of pain has been considered one of the characteristics of these rare lesions. In other reported cases, when pain has been present, it was minimal and never the presenting feature. The clinical and radiographic features and the surgical treatment are described. The pathogenesis is discussed and the literature reviewed. An addition to the surgical technique is given whereby one of the post operative complications may be obviated.", "PMID": 963639}
{"id": "pubmed23n0032_5645", "title": "Computer tomography in disseminated sclerosis.", "content": "A case is reported where the appearance of acute, diffuse, disseminated sclerosis on computer tomography (Ct scan) is described and the literature is reviewed. This disease may give rise to multiple, small areas of diminished X-ray absorption which may decrease in size during the course of the disease. The histological features of one of the lesions was correlated with the radiological findings. It is suggested that, perhaps, only during the active stage of demyelination can the lesions be detected on the Ct scan.", "contents": "Computer tomography in disseminated sclerosis. A case is reported where the appearance of acute, diffuse, disseminated sclerosis on computer tomography (Ct scan) is described and the literature is reviewed. This disease may give rise to multiple, small areas of diminished X-ray absorption which may decrease in size during the course of the disease. The histological features of one of the lesions was correlated with the radiological findings. It is suggested that, perhaps, only during the active stage of demyelination can the lesions be detected on the Ct scan.", "PMID": 963640}
{"id": "pubmed23n0032_5646", "title": "Focus on prevention.", "content": "Cardiovascular disease in Canada is estimated to cost over $1.2 billion each year. Advances made in the treatment of cardiovascular diseases have decreased the mortality rate associated with them, but the incidence of such diseases may nonetheless be termed epidemic. Leading epidemiologists suggest that control of this epidemic can only be attained through primary prevention....", "contents": "Focus on prevention. Cardiovascular disease in Canada is estimated to cost over $1.2 billion each year. Advances made in the treatment of cardiovascular diseases have decreased the mortality rate associated with them, but the incidence of such diseases may nonetheless be termed epidemic. Leading epidemiologists suggest that control of this epidemic can only be attained through primary prevention....", "PMID": 963680}
{"id": "pubmed23n0032_5647", "title": "A synthesis of 3',4'-dideoxykanamycin B.", "content": "3',4',-Dideoxykanamycin B, the kanamycin B derivative that is active against resistant bacteria, was prepared from kanamycin B via N-tosylation, 3',4'-O-sulphonylation, 3',4'-unsaturation, and hydrogenation. The unsaturated intermediate was obtained from the 3',4'-di-O-sulphonyl derivatives by the action of sodium iodide in N,N-dimethylformamide; if zinc dust was added in this reaction, aziridine derivatives were formed. Removal of the tosyl group was successfully performed by using sodium in ammonia-ethylamine.", "contents": "A synthesis of 3',4'-dideoxykanamycin B. 3',4',-Dideoxykanamycin B, the kanamycin B derivative that is active against resistant bacteria, was prepared from kanamycin B via N-tosylation, 3',4'-O-sulphonylation, 3',4'-unsaturation, and hydrogenation. The unsaturated intermediate was obtained from the 3',4'-di-O-sulphonyl derivatives by the action of sodium iodide in N,N-dimethylformamide; if zinc dust was added in this reaction, aziridine derivatives were formed. Removal of the tosyl group was successfully performed by using sodium in ammonia-ethylamine.", "PMID": 963688}
{"id": "pubmed23n0032_5648", "title": "Synthesis of the allo-analogue of trehalose.", "content": "Selective benzoylation of HO-2 and HO-2' of 4,6-O-benzylidene-alpha-D-glucopyranosyl 4,6-O-benzylidene-alpha-D-glucopyranoside with N-benzoylimidazole led to the exclusive formation of 2-O-benzoyl-4,6-O-benzylidene-alpha-D-glucopyranosyl 2-O-benzoyl-4,6-O-benzylidene-alpha-D-glucopyranoside. Oxidation of either the dibenzoate or the corresponding ditosylate with methyl sulphoxide--phosphorus pentaoxide gave the 3,3'-diulose, and subsequent reduction with borohydride gave the 3,3'diepimers having the allo-allo configuration. De-esterification and hydrolysis of the benzylidene substituents gave alpha-D-allopyranosyl alpha-D-allopyranoside.", "contents": "Synthesis of the allo-analogue of trehalose. Selective benzoylation of HO-2 and HO-2' of 4,6-O-benzylidene-alpha-D-glucopyranosyl 4,6-O-benzylidene-alpha-D-glucopyranoside with N-benzoylimidazole led to the exclusive formation of 2-O-benzoyl-4,6-O-benzylidene-alpha-D-glucopyranosyl 2-O-benzoyl-4,6-O-benzylidene-alpha-D-glucopyranoside. Oxidation of either the dibenzoate or the corresponding ditosylate with methyl sulphoxide--phosphorus pentaoxide gave the 3,3'-diulose, and subsequent reduction with borohydride gave the 3,3'diepimers having the allo-allo configuration. De-esterification and hydrolysis of the benzylidene substituents gave alpha-D-allopyranosyl alpha-D-allopyranoside.", "PMID": 963689}
{"id": "pubmed23n0032_5649", "title": "Synthesis of L-fucose 2-, 3-, and 4-sulphates.", "content": "Treatment of L-fucose with an excess pf pyridine-sulphur trioxide gave an equilibrium mixture of mono-, di-, and tri-sulphates. L-Fucose was sulphated under optimal conditions for monosulphate formation, and the monoester fraction was isolated by chromatography on DEAE-cellulose. The isomeric L-fucose 2-, 3-, and 4-sulphates (1-3) were separated on a DEAE-cellulose column by elution with borate buffer. The structures of 1-3 were established by electrophoresis, colour tests, periodate oxidation, and, for the 2-isomer, by comparison with a specimen of 1 that had been definitively synthesised via methyl 3,4-O-isopropylidene-alpha-L-fucopyranoside (6) and methyl alpha-L-fucopyranoside 2-(barium sulphate) (5). The latter was rapidly hydrolysed in hot, dilute acetic acid to 1 and methyl alpha-L-fucopyranoside (4).", "contents": "Synthesis of L-fucose 2-, 3-, and 4-sulphates. Treatment of L-fucose with an excess pf pyridine-sulphur trioxide gave an equilibrium mixture of mono-, di-, and tri-sulphates. L-Fucose was sulphated under optimal conditions for monosulphate formation, and the monoester fraction was isolated by chromatography on DEAE-cellulose. The isomeric L-fucose 2-, 3-, and 4-sulphates (1-3) were separated on a DEAE-cellulose column by elution with borate buffer. The structures of 1-3 were established by electrophoresis, colour tests, periodate oxidation, and, for the 2-isomer, by comparison with a specimen of 1 that had been definitively synthesised via methyl 3,4-O-isopropylidene-alpha-L-fucopyranoside (6) and methyl alpha-L-fucopyranoside 2-(barium sulphate) (5). The latter was rapidly hydrolysed in hot, dilute acetic acid to 1 and methyl alpha-L-fucopyranoside (4).", "PMID": 963690}
{"id": "pubmed23n0032_5650", "title": "An approach to the synthesis of branched-chain amino sugars from C-methylene sugars.", "content": "The reaction of 1,2:5,6-di-O-isopropylidene-3-C-methylene-alpha-D-ribo-hexofuranose (4) with mercuric azide in hot 50% aqueous tetrahydrofuran yielded, after reductive demercuration, 3-azido-3-deoxy-1,2:5,6-di-O-isopropylidene-3-C-methyl-alpha-D-glucofuranose (5). Partial, acid hydrolysis of 5 afforded the diol 7, which gave 3-azido-3-deoxy-1,2-O-isopropylidene-5,6-di-O-methanesulphonyl-3-C-methyl-alpha-D-glucofuranose (8) on sulphonylation. On hydrogenation over a platinum catalyst and N-acetylation, the dimethanesulphonate 8 furnished 3,6-acetyleprimino-3,6-dideoxy-1,2-O-isopropylidene-5-O-methanesulphonyl-3-C-methyl-alpha-D-glucofuranose (9), which was also prepared by an analogous sequence of reactions on 3-azido-3-deoxy-1,2-O-isopropylidene-5-O-methanesulphonyl-3-C-methyl-6-O-toluene-p-sulphonyl-alpha-D-glucofuranose (13). The formation of the N-acetylepimine 9 establishes the D-gluco configuration for 5. 1,2-O-Isopropylidene-3-C-methylene-alpha-D-ribo-hexofuranose (20) reacted with mercuric azide in aqueous tetrahydrofuran at approximately 85 degrees to give 3,6-anhydro-1,2-O-isopropylidene-3-C-methyl-alpha-D-glucofuranose (22) as a result of intramolecular participation by the C-6 hydroxyl group in the initial intermediate.", "contents": "An approach to the synthesis of branched-chain amino sugars from C-methylene sugars. The reaction of 1,2:5,6-di-O-isopropylidene-3-C-methylene-alpha-D-ribo-hexofuranose (4) with mercuric azide in hot 50% aqueous tetrahydrofuran yielded, after reductive demercuration, 3-azido-3-deoxy-1,2:5,6-di-O-isopropylidene-3-C-methyl-alpha-D-glucofuranose (5). Partial, acid hydrolysis of 5 afforded the diol 7, which gave 3-azido-3-deoxy-1,2-O-isopropylidene-5,6-di-O-methanesulphonyl-3-C-methyl-alpha-D-glucofuranose (8) on sulphonylation. On hydrogenation over a platinum catalyst and N-acetylation, the dimethanesulphonate 8 furnished 3,6-acetyleprimino-3,6-dideoxy-1,2-O-isopropylidene-5-O-methanesulphonyl-3-C-methyl-alpha-D-glucofuranose (9), which was also prepared by an analogous sequence of reactions on 3-azido-3-deoxy-1,2-O-isopropylidene-5-O-methanesulphonyl-3-C-methyl-6-O-toluene-p-sulphonyl-alpha-D-glucofuranose (13). The formation of the N-acetylepimine 9 establishes the D-gluco configuration for 5. 1,2-O-Isopropylidene-3-C-methylene-alpha-D-ribo-hexofuranose (20) reacted with mercuric azide in aqueous tetrahydrofuran at approximately 85 degrees to give 3,6-anhydro-1,2-O-isopropylidene-3-C-methyl-alpha-D-glucofuranose (22) as a result of intramolecular participation by the C-6 hydroxyl group in the initial intermediate.", "PMID": 963692}
{"id": "pubmed23n0032_5651", "title": "G.L.C. of the O-trimethylsilyl derivatives of hexuronic acids.", "content": "The free acids, sodium salts, and lactones of several hexuronic acids have been studied as their O-trimethylsilyl derivatives by gas-liquid chromatography using SE-30 and XE-60 liquid phases. Silylation was best performed in methyl sulphoxide. The equilibrium between the various forms of a hexuronic acid in methyl sulphoxide was also studied by g.l.c. following silylation. The hexamethyldisilazane used in the silylation disturbed the equilibrium attained in the solvent, but this was overcome by premixing the hexamethyldisilazane with chlorotrimethylsilane. Methyl sulphoxide and the silylating reagents gave a two-phase system in which the derivative was favourably partitioned into the upper layer. Partition coefficients and stabilities of the derivatives were measured, and a g.l.c. method for the analysis of the hexuronic acids was thereby developed. The oximes of the hexuronic acids were studied as alternative derivatives for g.l.c., and their equilibrium compositions and g.l.c. retention times are recorded.", "contents": "G.L.C. of the O-trimethylsilyl derivatives of hexuronic acids. The free acids, sodium salts, and lactones of several hexuronic acids have been studied as their O-trimethylsilyl derivatives by gas-liquid chromatography using SE-30 and XE-60 liquid phases. Silylation was best performed in methyl sulphoxide. The equilibrium between the various forms of a hexuronic acid in methyl sulphoxide was also studied by g.l.c. following silylation. The hexamethyldisilazane used in the silylation disturbed the equilibrium attained in the solvent, but this was overcome by premixing the hexamethyldisilazane with chlorotrimethylsilane. Methyl sulphoxide and the silylating reagents gave a two-phase system in which the derivative was favourably partitioned into the upper layer. Partition coefficients and stabilities of the derivatives were measured, and a g.l.c. method for the analysis of the hexuronic acids was thereby developed. The oximes of the hexuronic acids were studied as alternative derivatives for g.l.c., and their equilibrium compositions and g.l.c. retention times are recorded.", "PMID": 963693}
{"id": "pubmed23n0032_5652", "title": "Reaction of derivatives of methyl 2,3-O-benzylidene-6-deoxy-alpha-L-mannopyranoside with butyllithium: synthesis of methyl 2,6-dideoxy-4-O-methyl-alpha-L-erythro-hexopyranosid-3-ulose.", "content": "Methyl 2,3-O-benzylidene-6-deoxy-alpha-L-mannopyranoside (2) reacted with butyllithium to give a mixture of 1,5-anhydro-3-C-butyl-1,2,6-trideoxy-L-ribo-hex-1-enitol (3) and its L-arabino analogue (4), together with methyl 2,3,6-trideoxy-alpha-L-erythro-hex-2-enopyranoside (5). In contrast, the 4-O-methyl-analogue (8) of 2 was converted by butyllithium into methyl 2,6-dideoxy-4-O-methyl-alpha-L-erythro-hexo-pyranosid-3-ulose (9), which was further characterized as its oxime 10. The 4-O-benzyl analogue of 8, obtained as two separate diastereoisomers (6 and 7) differing in configuration at C-2 of the dioxolane ring gave a complex misture of products on treatment with butyllithium.", "contents": "Reaction of derivatives of methyl 2,3-O-benzylidene-6-deoxy-alpha-L-mannopyranoside with butyllithium: synthesis of methyl 2,6-dideoxy-4-O-methyl-alpha-L-erythro-hexopyranosid-3-ulose. Methyl 2,3-O-benzylidene-6-deoxy-alpha-L-mannopyranoside (2) reacted with butyllithium to give a mixture of 1,5-anhydro-3-C-butyl-1,2,6-trideoxy-L-ribo-hex-1-enitol (3) and its L-arabino analogue (4), together with methyl 2,3,6-trideoxy-alpha-L-erythro-hex-2-enopyranoside (5). In contrast, the 4-O-methyl-analogue (8) of 2 was converted by butyllithium into methyl 2,6-dideoxy-4-O-methyl-alpha-L-erythro-hexo-pyranosid-3-ulose (9), which was further characterized as its oxime 10. The 4-O-benzyl analogue of 8, obtained as two separate diastereoisomers (6 and 7) differing in configuration at C-2 of the dioxolane ring gave a complex misture of products on treatment with butyllithium.", "PMID": 963694}
{"id": "pubmed23n0032_5653", "title": "Synthesis of benzyl and allyl ethers of D-glucopyranose.", "content": "Starting from allyl 3-O-benzyl-4,6-O-benzylidene-alpha-D-glucopyranoside as a key intermediate, the following crystalline compounds were prepared: 2-O-allyl-3,4,6-tri-O-benzyl-D-glucopyranose (11) and its p-nitrobenzoate; 2,3,5-tri-O-benzyl-D-arabinofuranose (12) and the corresponding arabinitol; allyl 3,4,6-tri-O-benzyl-alpha-D-glucopyranoside (7); 3,4,6-tri-o-benzyl-D-glucopyranose (8); 2-0-allyl 3, 4-di-o-benzyl-D-glucopyranose (17) and its bis(p-nitrobenzoate); and 3,4-di-O-benzyl-D-glucopyranose (19). The p-nitrobenzoates of compounds 11 and 17 are potential intermediates for the synthesis of the glycolipids of the cytoplasmic membranes of Streptococci.", "contents": "Synthesis of benzyl and allyl ethers of D-glucopyranose. Starting from allyl 3-O-benzyl-4,6-O-benzylidene-alpha-D-glucopyranoside as a key intermediate, the following crystalline compounds were prepared: 2-O-allyl-3,4,6-tri-O-benzyl-D-glucopyranose (11) and its p-nitrobenzoate; 2,3,5-tri-O-benzyl-D-arabinofuranose (12) and the corresponding arabinitol; allyl 3,4,6-tri-O-benzyl-alpha-D-glucopyranoside (7); 3,4,6-tri-o-benzyl-D-glucopyranose (8); 2-0-allyl 3, 4-di-o-benzyl-D-glucopyranose (17) and its bis(p-nitrobenzoate); and 3,4-di-O-benzyl-D-glucopyranose (19). The p-nitrobenzoates of compounds 11 and 17 are potential intermediates for the synthesis of the glycolipids of the cytoplasmic membranes of Streptococci.", "PMID": 963695}
{"id": "pubmed23n0032_5654", "title": "Structure of the levan elaborated by Streptococcus salivarius strain 51: an application of chemical-ionisation mass-spectrometry.", "content": "The polysaccharide elaborated by Streptococcus salivarius strain 51 contains beta-D-fructofuranose residues linked through positions 2 and 6, as well as 1, 2, and 6. The approximate numbers of terminal, non-reducing D-fructofuranose residues and those linked through positions 2 and 6, and through 1, 2, and 6 in the average repeating-unit are 1, 7, and 1, respectively. The branches through the beta-(2 leads to 1)-linkage contain up to at least four D-fructofuranose residues. Chemical-ionisation mass-spectrometry aids the assignment of structures to O-acetyl-O-methylalditols obtained in methylation analysis.", "contents": "Structure of the levan elaborated by Streptococcus salivarius strain 51: an application of chemical-ionisation mass-spectrometry. The polysaccharide elaborated by Streptococcus salivarius strain 51 contains beta-D-fructofuranose residues linked through positions 2 and 6, as well as 1, 2, and 6. The approximate numbers of terminal, non-reducing D-fructofuranose residues and those linked through positions 2 and 6, and through 1, 2, and 6 in the average repeating-unit are 1, 7, and 1, respectively. The branches through the beta-(2 leads to 1)-linkage contain up to at least four D-fructofuranose residues. Chemical-ionisation mass-spectrometry aids the assignment of structures to O-acetyl-O-methylalditols obtained in methylation analysis.", "PMID": 963696}
{"id": "pubmed23n0032_5655", "title": "A 13C-N.M.R. spectral study of chondroitin sulfates A, B, and C: evidence of heterogeneity.", "content": "Chondroitin sulfates A, B, and C produce well-resolved 13C-n.m.r. spectra which allow for a more complete characterization than that available from their p.m.r. spectra. The 13C data fully support earlier evidence as to the main structural features of these glycosaminoglycans, but they also show that many chondroitin preparations are substantially heterogeneous in composition. Thus, spectra of chondroitin A and C have the appearance of composites representative of both types of polymer: specimens of A may contain 25% of the C-type of structural sequence, and C,30% of the A-type of sequence; 10-20% of unidentified constituents, including a residue bearing a 6-sulfate group, are present in the specimens of chondroitin B. Chemical-shift and 1JC-H values found for the L-iduronic acid residues of chondroitin B, as well as the effect of gadolinium nitrate on the relaxation properties of its 13C nuclei, indicate that this moiety possesses the alpha configuration and favors the 1C4(L) conformation. Corresponding data for the acetamidodeoxy-D-galactose and D-glucuronic acid residues of the chondroitins are consistent with the beta-anomeric configuration and 4C1(D) conformation in all instances.", "contents": "A 13C-N.M.R. spectral study of chondroitin sulfates A, B, and C: evidence of heterogeneity. Chondroitin sulfates A, B, and C produce well-resolved 13C-n.m.r. spectra which allow for a more complete characterization than that available from their p.m.r. spectra. The 13C data fully support earlier evidence as to the main structural features of these glycosaminoglycans, but they also show that many chondroitin preparations are substantially heterogeneous in composition. Thus, spectra of chondroitin A and C have the appearance of composites representative of both types of polymer: specimens of A may contain 25% of the C-type of structural sequence, and C,30% of the A-type of sequence; 10-20% of unidentified constituents, including a residue bearing a 6-sulfate group, are present in the specimens of chondroitin B. Chemical-shift and 1JC-H values found for the L-iduronic acid residues of chondroitin B, as well as the effect of gadolinium nitrate on the relaxation properties of its 13C nuclei, indicate that this moiety possesses the alpha configuration and favors the 1C4(L) conformation. Corresponding data for the acetamidodeoxy-D-galactose and D-glucuronic acid residues of the chondroitins are consistent with the beta-anomeric configuration and 4C1(D) conformation in all instances.", "PMID": 963697}
{"id": "pubmed23n0032_5656", "title": "Some properties of a fungal beta-D-glucanase preparation.", "content": "A commercial enzyme preparation, of fungal origin, contained a mixture of beta-D-glucanases which were fractionated by ion-exchange chromatography to give a mixture of an endo-(1 leads to 4)- and an exo-(1 leads to 3)-beta-D-glucanase. These two enzymes were then separated by molecular-sieve chromatography on Sephadex G-150. The purified exo-(1 leads to 3)-beta-D-glucanase has a relatively high specificity for (1 leads to 3)-beta-D-glucosidic linkages, and has no action on lichenin.", "contents": "Some properties of a fungal beta-D-glucanase preparation. A commercial enzyme preparation, of fungal origin, contained a mixture of beta-D-glucanases which were fractionated by ion-exchange chromatography to give a mixture of an endo-(1 leads to 4)- and an exo-(1 leads to 3)-beta-D-glucanase. These two enzymes were then separated by molecular-sieve chromatography on Sephadex G-150. The purified exo-(1 leads to 3)-beta-D-glucanase has a relatively high specificity for (1 leads to 3)-beta-D-glucosidic linkages, and has no action on lichenin.", "PMID": 963698}
{"id": "pubmed23n0032_5657", "title": "Preparation and characterization of a dextran-amylase conjugate.", "content": "Bacillus amyloliquefaciens alpha-amylase was attached to dextran after activation of the polysaccharide by using a modification of the cyanogen bromide method. The soluble dextran-amylase conjugate was purified by molecular-sieve chromatography. The conjugated enzyme has greater stability than the unmodified enzyme at low pH values, during heat treatment, and on removal of calcium ions with a chelating agent. Attachment of dextran to alpha-amylase did not alter the Michaelis constant of the enzyme acting on starch. The polysaccharide-enzyme conjugate probably consists of a cross-linked aggregate of many dextran and many enzyme molecules, in which a proportion of the enzyme molecules, although not inactivated, are unable to express their activity, except after dextranase treatment.", "contents": "Preparation and characterization of a dextran-amylase conjugate. Bacillus amyloliquefaciens alpha-amylase was attached to dextran after activation of the polysaccharide by using a modification of the cyanogen bromide method. The soluble dextran-amylase conjugate was purified by molecular-sieve chromatography. The conjugated enzyme has greater stability than the unmodified enzyme at low pH values, during heat treatment, and on removal of calcium ions with a chelating agent. Attachment of dextran to alpha-amylase did not alter the Michaelis constant of the enzyme acting on starch. The polysaccharide-enzyme conjugate probably consists of a cross-linked aggregate of many dextran and many enzyme molecules, in which a proportion of the enzyme molecules, although not inactivated, are unable to express their activity, except after dextranase treatment.", "PMID": 963699}
{"id": "pubmed23n0032_5658", "title": "The agar-type polysaccharide from the red alga Ceramium rubrum.", "content": "Aqueous extraction of the red alga C. rubrum gave a galactan sulphate and, possibly, a separate glucan and xylan. The galactan sulphate has an alternating structure of the agar-type with D-galactose or 6-O-methyl-D-galactose as one alternating unit, and L-galactose, 3,6-anhydro-L-galactose, and their respective 2-methyl ethers as the other unit. Sulphate hemi-ester groups are present on position 6 of both D- and L-galactose residues, with smaller amounts on positions 2 and 4 of, probably, D-galactose residues. The polysaccharide differs from others previously examined in that most of the L-galactose residues are non-sulphated.", "contents": "The agar-type polysaccharide from the red alga Ceramium rubrum. Aqueous extraction of the red alga C. rubrum gave a galactan sulphate and, possibly, a separate glucan and xylan. The galactan sulphate has an alternating structure of the agar-type with D-galactose or 6-O-methyl-D-galactose as one alternating unit, and L-galactose, 3,6-anhydro-L-galactose, and their respective 2-methyl ethers as the other unit. Sulphate hemi-ester groups are present on position 6 of both D- and L-galactose residues, with smaller amounts on positions 2 and 4 of, probably, D-galactose residues. The polysaccharide differs from others previously examined in that most of the L-galactose residues are non-sulphated.", "PMID": 963701}
{"id": "pubmed23n0032_5659", "title": "Structural investigations of the extracellular polysaccharide elaborated by S19, a Xanthomonas-type bacterium.", "content": "The extracellular, acidic heteropolysaccharide from Xanthomonas S19 consists of D-glucuronic acid, D-glucose, D-galactose, and D-mannose residues in the approximate molar ratios of 1.6:3:1:1, plus acetyl groups linked to C-2 and/or C-3 of a large proportion of the glucose residues. Methylation studies showed that the glucose is present as non-reducing end-group also as 1,2- and 1,4-linked units, the galactose residues are solely 1,3-linked, a major proportion of the mannose residues are 1,2,4-linked and the rest 1,2-linked. A high proportion of the glucuronic acid units are 1,4-linked. Periodate oxidation confirmed the presence of these linkages. The disaccharides D-Glc-(1 leads to 4)-D-Glc, D-Glc-(1 leads to 2)-D-Man, D-Glc-(1 leads to 3)-D-Gal, D-Gal(1 leads to 2)-D-Glc, D-GlcA-(1 leads to 4)-D-GlcA, and beta-D-GlcA-(1 leads to 4)-D-Man were isolated from a partial hydrolysate of the polysaccharide, and characterised. The similarities and differences between this polysaccharide and those from other Xanthomonas species are discussed.", "contents": "Structural investigations of the extracellular polysaccharide elaborated by S19, a Xanthomonas-type bacterium. The extracellular, acidic heteropolysaccharide from Xanthomonas S19 consists of D-glucuronic acid, D-glucose, D-galactose, and D-mannose residues in the approximate molar ratios of 1.6:3:1:1, plus acetyl groups linked to C-2 and/or C-3 of a large proportion of the glucose residues. Methylation studies showed that the glucose is present as non-reducing end-group also as 1,2- and 1,4-linked units, the galactose residues are solely 1,3-linked, a major proportion of the mannose residues are 1,2,4-linked and the rest 1,2-linked. A high proportion of the glucuronic acid units are 1,4-linked. Periodate oxidation confirmed the presence of these linkages. The disaccharides D-Glc-(1 leads to 4)-D-Glc, D-Glc-(1 leads to 2)-D-Man, D-Glc-(1 leads to 3)-D-Gal, D-Gal(1 leads to 2)-D-Glc, D-GlcA-(1 leads to 4)-D-GlcA, and beta-D-GlcA-(1 leads to 4)-D-Man were isolated from a partial hydrolysate of the polysaccharide, and characterised. The similarities and differences between this polysaccharide and those from other Xanthomonas species are discussed.", "PMID": 963702}
{"id": "pubmed23n0032_5660", "title": "[Complete structure of three galactoxyloglucans (amyloids) from seeds].", "content": "D-Galacto-D-xylo-D-glucans (amyloids) from Balsamina, Tropaeolum, and Tamarindus seeds behave in a similar manner in the presence of various glycosidase preparations: slow depolymerization by enzymes from several germinated or non-germinated seeds, and hydrolysis into monosaccharides and oligosaccharides by commercial cellulase and hemicellulase preparations from fungi. A purified cellulase from Penicillium notatum gave a dialyzable fraction almost exclusively composed of alpha-D-xylopyranosyl-(1 leads to 6)-D-glucose residues and a nondialyzable fraction composed of chains of beta-D-(1 leads to 4) [With some (1 leads to 3)]-glucopyranosyl residues; beta-D-galactopyranosyl-(1 leads to 2)-alpha-D-xylosyl groups are linked to some of the beta-D-glucosyl residues at O-6. The presence of (1 leads to 3)-linkages in the D-glucan chain of the Balsamina was verified by methylation and sequential periodate oxidation-borohydride reduction; the distribution of the substituents on the D-glucan chain is not regular. The main D-glucan backbone, where the  beta-D-glucosyl residues are partly linked at O-6 to beta-D-galactosyl-(1 leads to 2)-D-xylosyl groups, is linked to D-glucan chains where almost all the D-glucose units are linked at O-6 by one alpha-D-xylosyl group. The presence of 3,6-di-O-methyl-D-glucose after permethylation and hydrolysis suggests that the xyloglucan chains are linked to O-2 of the D-glucosyl units of the galactoxyloglucan backbone.", "contents": "[Complete structure of three galactoxyloglucans (amyloids) from seeds]. D-Galacto-D-xylo-D-glucans (amyloids) from Balsamina, Tropaeolum, and Tamarindus seeds behave in a similar manner in the presence of various glycosidase preparations: slow depolymerization by enzymes from several germinated or non-germinated seeds, and hydrolysis into monosaccharides and oligosaccharides by commercial cellulase and hemicellulase preparations from fungi. A purified cellulase from Penicillium notatum gave a dialyzable fraction almost exclusively composed of alpha-D-xylopyranosyl-(1 leads to 6)-D-glucose residues and a nondialyzable fraction composed of chains of beta-D-(1 leads to 4) [With some (1 leads to 3)]-glucopyranosyl residues; beta-D-galactopyranosyl-(1 leads to 2)-alpha-D-xylosyl groups are linked to some of the beta-D-glucosyl residues at O-6. The presence of (1 leads to 3)-linkages in the D-glucan chain of the Balsamina was verified by methylation and sequential periodate oxidation-borohydride reduction; the distribution of the substituents on the D-glucan chain is not regular. The main D-glucan backbone, where the  beta-D-glucosyl residues are partly linked at O-6 to beta-D-galactosyl-(1 leads to 2)-D-xylosyl groups, is linked to D-glucan chains where almost all the D-glucose units are linked at O-6 by one alpha-D-xylosyl group. The presence of 3,6-di-O-methyl-D-glucose after permethylation and hydrolysis suggests that the xyloglucan chains are linked to O-2 of the D-glucosyl units of the galactoxyloglucan backbone.", "PMID": 963703}
{"id": "pubmed23n0032_5661", "title": "Neighbouring acetamido-group participation in reactions of derivatives of 2-acetamido-2-deoxy-D-glucose.", "content": "Kinetic measurements suggest that neighbouring acetamido-group participation occurs in the spontaneous hydrolysis and methanolysis of o-carboxyphenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and in the spontaneous hydrolysis of 2,4-dinitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and 2-acetamido-2-deoxy-beta-D-glycopyranosyl fluoride. The methanolyses of these compounds proceed with predominant retention of configuration which is also consistent with neighbouring acetamido-group participation. The oxazoline intermediate which would arise from such a process was detected during methanolysis of 2-acetamido-2-deoxy-beta-D-glucopyranosyl fluoride in the presence of bases by n.m.r., i.r., and u.v. spectroscopy. Attempts to isolate the oxazoline were unsuccessful.", "contents": "Neighbouring acetamido-group participation in reactions of derivatives of 2-acetamido-2-deoxy-D-glucose. Kinetic measurements suggest that neighbouring acetamido-group participation occurs in the spontaneous hydrolysis and methanolysis of o-carboxyphenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and in the spontaneous hydrolysis of 2,4-dinitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and 2-acetamido-2-deoxy-beta-D-glycopyranosyl fluoride. The methanolyses of these compounds proceed with predominant retention of configuration which is also consistent with neighbouring acetamido-group participation. The oxazoline intermediate which would arise from such a process was detected during methanolysis of 2-acetamido-2-deoxy-beta-D-glucopyranosyl fluoride in the presence of bases by n.m.r., i.r., and u.v. spectroscopy. Attempts to isolate the oxazoline were unsuccessful.", "PMID": 963707}
{"id": "pubmed23n0032_5662", "title": "The ventral lateral geniculate nucleus, pars lateralis of the rat. Synaptic organization and conditions for axonal sprouting.", "content": "The synaptic organization of the pars lateralis portion of the ventral lateral geniculate nucleus is similar to that of other thalamic nuclei. There are four types of synaptic knobs (RL, RS, F1, F2). RL knobs are large and irregularly shaped, contain round synaptic vesicles and make multiple asymmetrical junctions. They are found primarily in \"synaptic islands\" making contact with gemmules, spines, small dendrites, and other synaptic profiles containing pleiomorphic synaptic vesicles (F2). Smaller RS knobs contain round vesicles and make asymmetrical junctions with the same type of elements as RL knobs, with the exception of the F2 profiles, but are seldom found in synaptic islands. F1 knobs contain flattened synaptic vesicles and form symmetrical junctions with F2 knobs, gemmules, spines, and small-medium dendrites in synaptic islands, throughout the neuropil, and on the proximal dendrites and soma of the largest type of neuron. F2 knobs are irregularly shaped, contain pleiomorphic synaptic vesicles and make symmetrical junctions primarily with gemmules and spines in synaptic islands. They are postsynaptic to RL and F1 knobs. Occipital decortication indicates that cortical terminals are of the RS type. Bilateral enucleation indicates that retinal terminals are of both the FL and RS type. The large amount of geographic overlap of retinal and cortical terminals on gemmules, spines, and small dendrites found in the neuropil outside of synaptic islands logically would maximize axonal sprouting between these two sources.", "contents": "The ventral lateral geniculate nucleus, pars lateralis of the rat. Synaptic organization and conditions for axonal sprouting. The synaptic organization of the pars lateralis portion of the ventral lateral geniculate nucleus is similar to that of other thalamic nuclei. There are four types of synaptic knobs (RL, RS, F1, F2). RL knobs are large and irregularly shaped, contain round synaptic vesicles and make multiple asymmetrical junctions. They are found primarily in \"synaptic islands\" making contact with gemmules, spines, small dendrites, and other synaptic profiles containing pleiomorphic synaptic vesicles (F2). Smaller RS knobs contain round vesicles and make asymmetrical junctions with the same type of elements as RL knobs, with the exception of the F2 profiles, but are seldom found in synaptic islands. F1 knobs contain flattened synaptic vesicles and form symmetrical junctions with F2 knobs, gemmules, spines, and small-medium dendrites in synaptic islands, throughout the neuropil, and on the proximal dendrites and soma of the largest type of neuron. F2 knobs are irregularly shaped, contain pleiomorphic synaptic vesicles and make symmetrical junctions primarily with gemmules and spines in synaptic islands. They are postsynaptic to RL and F1 knobs. Occipital decortication indicates that cortical terminals are of the RS type. Bilateral enucleation indicates that retinal terminals are of both the FL and RS type. The large amount of geographic overlap of retinal and cortical terminals on gemmules, spines, and small dendrites found in the neuropil outside of synaptic islands logically would maximize axonal sprouting between these two sources.", "PMID": 963724}
{"id": "pubmed23n0032_5663", "title": "Spermatogenesis of a marine snail, Littorina sitkana.", "content": "The fine structure of the spermatogonium, spermatocyte and spermatid of a marine snail, Littorina sitkana is described. The ring centriole (annulus) is formed from the distal centriole and it migrates to the base of the mitochondrial region where it lies in a joint-like structure which is formed by an area of invaginated plasma membrane. The distal and proximal centrioles are at first perpendicular to each other but the proximal centriole rotates to a position coaxial with the distal centriole and fuses with it. The peripheral doublet fibers are continuous between the two centrioles but the central fibers originate only in the distal centriole. The acrosome differentiates from the proacrosomal granule which is derived from a Golgi body. Microtubules, present at this stage, may assist acrosomal formation. Chromatin condensation begins with the formation of fibrous strands, then to lamellar plates which become folded and later twisted around the flagellar shaft. In the final stages the lamellae appear in cross section as concentric rings which eventually fuse to form a homogeneously dense nuclear tube.", "contents": "Spermatogenesis of a marine snail, Littorina sitkana. The fine structure of the spermatogonium, spermatocyte and spermatid of a marine snail, Littorina sitkana is described. The ring centriole (annulus) is formed from the distal centriole and it migrates to the base of the mitochondrial region where it lies in a joint-like structure which is formed by an area of invaginated plasma membrane. The distal and proximal centrioles are at first perpendicular to each other but the proximal centriole rotates to a position coaxial with the distal centriole and fuses with it. The peripheral doublet fibers are continuous between the two centrioles but the central fibers originate only in the distal centriole. The acrosome differentiates from the proacrosomal granule which is derived from a Golgi body. Microtubules, present at this stage, may assist acrosomal formation. Chromatin condensation begins with the formation of fibrous strands, then to lamellar plates which become folded and later twisted around the flagellar shaft. In the final stages the lamellae appear in cross section as concentric rings which eventually fuse to form a homogeneously dense nuclear tube.", "PMID": 963725}
{"id": "pubmed23n0032_5664", "title": "Inhibition of 5'-nucleotidase by concanavalin A: evidence for localization on the outer surface of the plasma membrane.", "content": "5'-Nucleotidase activity of rat liver plasma membrane is markedly inhibited by concanavalin A. Taken together with a unilateral pattern of labelling of concanavalin A binding sites with hemocyanin, the results indicate that an allosteric site of the enzyme is at the outer surface of the membrane.", "contents": "Inhibition of 5'-nucleotidase by concanavalin A: evidence for localization on the outer surface of the plasma membrane. 5'-Nucleotidase activity of rat liver plasma membrane is markedly inhibited by concanavalin A. Taken together with a unilateral pattern of labelling of concanavalin A binding sites with hemocyanin, the results indicate that an allosteric site of the enzyme is at the outer surface of the membrane.", "PMID": 963726}
{"id": "pubmed23n0032_5665", "title": "Gap junctions between astrocytes during growth and differentiation in organ culture systems.", "content": "Fetal rat neocortex maintained in organ culture systems with the use of sponge foam matrices and millipore filter platforms undergoes growth and cytodifferentiation along classical neuronal and glial lines up to 36 days in vitro (DIV). Astrocytic differentiation is characterized by accumulation of 80-90 A glial filaments in the cell bodies and processes of astrocytes. Gap or nexus junctions closely resembling those formed in mammalian brain in situ are identified by 15 DIV. By 36 DIV, interastrocytic gap junctions are numerous and frequently join extensive lengths of adjacent glial plasma membranes. The results suggest that these organ culture systems may provide a favorable environment for the study of cellular structure and function of coupled neuroglia.", "contents": "Gap junctions between astrocytes during growth and differentiation in organ culture systems. Fetal rat neocortex maintained in organ culture systems with the use of sponge foam matrices and millipore filter platforms undergoes growth and cytodifferentiation along classical neuronal and glial lines up to 36 days in vitro (DIV). Astrocytic differentiation is characterized by accumulation of 80-90 A glial filaments in the cell bodies and processes of astrocytes. Gap or nexus junctions closely resembling those formed in mammalian brain in situ are identified by 15 DIV. By 36 DIV, interastrocytic gap junctions are numerous and frequently join extensive lengths of adjacent glial plasma membranes. The results suggest that these organ culture systems may provide a favorable environment for the study of cellular structure and function of coupled neuroglia.", "PMID": 963727}
{"id": "pubmed23n0032_5666", "title": "A scanning electron microscope study of the development of free axonal sprouts at the cut ends of dorsal spinal nerve roots in the rat.", "content": "Rat dorsal spinal nerve roots were cut and the tip on the ganglionic side of the cut was examined by scanning electron microscopy at 0, 7, 20 and 48 h after operation. Seven hours after cutting, free axonal sprouts had started to protrude from the cut end of the nerve. After 20 h the free sprouts were more profuse than at 7 h but were smaller and had a rougher surface. At both 7 and 20 h many of the sprouts consisted of a stalk 2-7 mum in diameter with a bulbous end 5-20 mum in diameter. A few branching sprouts were seen. At 48 h the sprouts were shrunken with a deeply furrowed surface. The significance of the surface structure of the sprouts is discussed.", "contents": "A scanning electron microscope study of the development of free axonal sprouts at the cut ends of dorsal spinal nerve roots in the rat. Rat dorsal spinal nerve roots were cut and the tip on the ganglionic side of the cut was examined by scanning electron microscopy at 0, 7, 20 and 48 h after operation. Seven hours after cutting, free axonal sprouts had started to protrude from the cut end of the nerve. After 20 h the free sprouts were more profuse than at 7 h but were smaller and had a rougher surface. At both 7 and 20 h many of the sprouts consisted of a stalk 2-7 mum in diameter with a bulbous end 5-20 mum in diameter. A few branching sprouts were seen. At 48 h the sprouts were shrunken with a deeply furrowed surface. The significance of the surface structure of the sprouts is discussed.", "PMID": 963728}
{"id": "pubmed23n0032_5667", "title": "Histochemical light microscopic study of catecholamine containing paraganglia in the human pelvis.", "content": "Histological and histochemical techniques have been employed to determine the structure and autonomic innervation of paraganglia located in the human pelvis. In foetal and early postnatal tissues, paraganglia formed rounded cellular masses which were frequently in company with the autonomic  nerves and ganglia of the urinary bladder and other pelvic viscera. The constituent cells contained only small amounts of cholinesterase and were unrelated to enzyme positive autonomic nerves; acetylcholinesterase containing nerves were occasionally observed in the capsule and the fibrous septa of the pelvic paraganglia. In early postnatal specimens, pelvic paraganglia frequently contained single or multiple \"pacinian-like\" corpuscles, each possessing a central region which was rich in both acetyl and pseudocholinesterase. These structures were rarely observed within autonomic ganglia and were absent 4 1/2 years post partum. By means of a histochemical technique, pelvic paraganglia were found to contain catecholamine which was attributed to the presence of relatively large quantities of noradrenaline. These observations have been discussed with particular reference to the results of other studies on the autonomic innervation of paraganglia.", "contents": "Histochemical light microscopic study of catecholamine containing paraganglia in the human pelvis. Histological and histochemical techniques have been employed to determine the structure and autonomic innervation of paraganglia located in the human pelvis. In foetal and early postnatal tissues, paraganglia formed rounded cellular masses which were frequently in company with the autonomic  nerves and ganglia of the urinary bladder and other pelvic viscera. The constituent cells contained only small amounts of cholinesterase and were unrelated to enzyme positive autonomic nerves; acetylcholinesterase containing nerves were occasionally observed in the capsule and the fibrous septa of the pelvic paraganglia. In early postnatal specimens, pelvic paraganglia frequently contained single or multiple \"pacinian-like\" corpuscles, each possessing a central region which was rich in both acetyl and pseudocholinesterase. These structures were rarely observed within autonomic ganglia and were absent 4 1/2 years post partum. By means of a histochemical technique, pelvic paraganglia were found to contain catecholamine which was attributed to the presence of relatively large quantities of noradrenaline. These observations have been discussed with particular reference to the results of other studies on the autonomic innervation of paraganglia.", "PMID": 963729}
{"id": "pubmed23n0032_5668", "title": "Uptake of ferritin by the cephalopod optic gland.", "content": "Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporting that the ultrastructure of the organ is unusual for an endocrine gland.", "contents": "Uptake of ferritin by the cephalopod optic gland. Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporting that the ultrastructure of the organ is unusual for an endocrine gland.", "PMID": 963730}
{"id": "pubmed23n0032_5669", "title": "The ultrastructure of the integument of the American eel, Anguilla rostrata.", "content": "The morphology and ultrastructure of the lateral body integument of the leptocephalus, glass eel, pigmented elver, and adult stages of the American eel, Anguilla rostrata, were examined with light and electron microscopy. The integument consists of an epidermis separated by a basal lamina from the underlying dermis. Three cell types are present in the epidermis in all stages. Filament-containing cells, which are the principal structural cell type, are increasingly numerous at each stage. Mucous cells, which secrete the mucous that compose the mucous surface coat, are also more numerous in each subsequent stage and are more numerous in the anterior lateral body epidermis than in the posterior lateral body epidermis of the adult. Club cells, whose function is unknown, are most numerous in the glass eel and pigmented elver. Chloride cells are common in the leptocephalus which is marine and infrequent in the glass eel. They are not present in the pigmented elver and adult which inhabit estuaries and freshwater. Lymphocytes and melanocytes are also present in some stages. The dermis comprises two layers: a layer of collagenous lamellae, the stratum compactum, and an underlying layer of loose connective tissue, the stratum spongiosum. There is a progressive increase in epidermal thickness at each stage which is paralleled by an increase in the thickness of the stratum compactum. Rudimentary scales are present in the dermis of the adult. The increase in the number of epidermal filament-containing cells, epidermal thickness and stratum compactum thickness is correlated with an increased need for protection from abrasion and mechanical damage as the eel moves from a pelagic, oceanic habitat to a benthic, freshwater habitat. The increase in mucous cell numbers is likewise correlated with an increased need for the protective and anti-bacterial action of the mucous surface coat in the freshwater environment.", "contents": "The ultrastructure of the integument of the American eel, Anguilla rostrata. The morphology and ultrastructure of the lateral body integument of the leptocephalus, glass eel, pigmented elver, and adult stages of the American eel, Anguilla rostrata, were examined with light and electron microscopy. The integument consists of an epidermis separated by a basal lamina from the underlying dermis. Three cell types are present in the epidermis in all stages. Filament-containing cells, which are the principal structural cell type, are increasingly numerous at each stage. Mucous cells, which secrete the mucous that compose the mucous surface coat, are also more numerous in each subsequent stage and are more numerous in the anterior lateral body epidermis than in the posterior lateral body epidermis of the adult. Club cells, whose function is unknown, are most numerous in the glass eel and pigmented elver. Chloride cells are common in the leptocephalus which is marine and infrequent in the glass eel. They are not present in the pigmented elver and adult which inhabit estuaries and freshwater. Lymphocytes and melanocytes are also present in some stages. The dermis comprises two layers: a layer of collagenous lamellae, the stratum compactum, and an underlying layer of loose connective tissue, the stratum spongiosum. There is a progressive increase in epidermal thickness at each stage which is paralleled by an increase in the thickness of the stratum compactum. Rudimentary scales are present in the dermis of the adult. The increase in the number of epidermal filament-containing cells, epidermal thickness and stratum compactum thickness is correlated with an increased need for protection from abrasion and mechanical damage as the eel moves from a pelagic, oceanic habitat to a benthic, freshwater habitat. The increase in mucous cell numbers is likewise correlated with an increased need for the protective and anti-bacterial action of the mucous surface coat in the freshwater environment.", "PMID": 963731}
{"id": "pubmed23n0032_5670", "title": "A look into the cock-pit of the fly. The architecture of the lobular plate.", "content": "An anatomical investigation has been carried out on the third optic ganglion of the fly, Musca domestica. Two systems of giant units, the dendritic arborizations of which are arranged orthogonally relative to each other, dominate the neuropile of this ganglion. The elements of the two systems have been reconstructed using a graphical procedure based on histological sections. One system branches predominantly in the dorso-ventral direction, the other one in the anterior-posterior direction. Both systems of the giant units have a twin system composed of elements smaller in diameter and strictly parallel to the main units. The two systems have been termed the Vertical and Horizontal Systems. The elements of the two systems of fibers project into the periesophageal region where they come into contact with other descending elements. Electron microscopic investigations show that the two systems are post-synaptic at the level of the ganglion from which they originate. The horizontal system has been shown to be post and pre-synaptic in nature during its course in the mid-brain and ultimately presynaptic at its endings in the periesophageal ring. The peculiar geometric arrangement of the two anatomical systems of fibers suggests a precise function in relation to the visual world and in particular to the detection of the direction of motion. The accuracy of the structural pattern displayed by the giant units in the lobular plate seems to suggest that this optic ganglion represents the ultimate orderly projection of the external world in the brain of the fly. A short review of the electrophysiological data concerning this ganglion has been tentatively correlated with some behavioral data related to the visual orientation and fixation in insects.", "contents": "A look into the cock-pit of the fly. The architecture of the lobular plate. An anatomical investigation has been carried out on the third optic ganglion of the fly, Musca domestica. Two systems of giant units, the dendritic arborizations of which are arranged orthogonally relative to each other, dominate the neuropile of this ganglion. The elements of the two systems have been reconstructed using a graphical procedure based on histological sections. One system branches predominantly in the dorso-ventral direction, the other one in the anterior-posterior direction. Both systems of the giant units have a twin system composed of elements smaller in diameter and strictly parallel to the main units. The two systems have been termed the Vertical and Horizontal Systems. The elements of the two systems of fibers project into the periesophageal region where they come into contact with other descending elements. Electron microscopic investigations show that the two systems are post-synaptic at the level of the ganglion from which they originate. The horizontal system has been shown to be post and pre-synaptic in nature during its course in the mid-brain and ultimately presynaptic at its endings in the periesophageal ring. The peculiar geometric arrangement of the two anatomical systems of fibers suggests a precise function in relation to the visual world and in particular to the detection of the direction of motion. The accuracy of the structural pattern displayed by the giant units in the lobular plate seems to suggest that this optic ganglion represents the ultimate orderly projection of the external world in the brain of the fly. A short review of the electrophysiological data concerning this ganglion has been tentatively correlated with some behavioral data related to the visual orientation and fixation in insects.", "PMID": 963732}
{"id": "pubmed23n0032_5671", "title": "The ultrastructure of paraganglia associated with the inferior mesenteric ganglia in the guinea-pig.", "content": "The paraganglia of the inferior mesenteric ganglia in the guinea-pig are composed of small chromaffin cells containing an abundance of granule-containing vesicles. The chromaffin cells are almost completely surrounded by satellite cells. In areas in which satellite cell processes do not intervene, the membranes of adjacent chromaffin cells are closely apposed and often form specialized attachment zones. The paraganglia contain a dense capillary network, the endothelial cells of which are often extremely attenuated and show areas of fenestration. The processes of chromaffin cells approach close to the capillary walls and are often bare of satellite cells covering on the side facing the capillary. Evidence has been obtained for the exocytotic release of the contents of chromaffin cell vesicles into pericapillary spaces. Synapses of cholinergic and noradrenergic axons are seen on the chromaffin cells. The cholinergic axons degenerate when the praganglia are decentralized, but the noradrenergic axons, which appear to arise from the local inferior mesenteric ganglia, remain intact. The results suggest that the paraganglia have an endocrine function.", "contents": "The ultrastructure of paraganglia associated with the inferior mesenteric ganglia in the guinea-pig. The paraganglia of the inferior mesenteric ganglia in the guinea-pig are composed of small chromaffin cells containing an abundance of granule-containing vesicles. The chromaffin cells are almost completely surrounded by satellite cells. In areas in which satellite cell processes do not intervene, the membranes of adjacent chromaffin cells are closely apposed and often form specialized attachment zones. The paraganglia contain a dense capillary network, the endothelial cells of which are often extremely attenuated and show areas of fenestration. The processes of chromaffin cells approach close to the capillary walls and are often bare of satellite cells covering on the side facing the capillary. Evidence has been obtained for the exocytotic release of the contents of chromaffin cell vesicles into pericapillary spaces. Synapses of cholinergic and noradrenergic axons are seen on the chromaffin cells. The cholinergic axons degenerate when the praganglia are decentralized, but the noradrenergic axons, which appear to arise from the local inferior mesenteric ganglia, remain intact. The results suggest that the paraganglia have an endocrine function.", "PMID": 963733}
{"id": "pubmed23n0032_5672", "title": "Electron microscopic immunocytochemical demonstration of separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres in the neural lobe of the rat hypophysis.", "content": "By means of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the electron microscopic level, it was demonstrated that the hormones of the neural lobe of the rat hypophysis are located in separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres. These observations confirm the results of our previous immunocytochemical studies at the light microscopic level.", "contents": "Electron microscopic immunocytochemical demonstration of separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres in the neural lobe of the rat hypophysis. By means of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the electron microscopic level, it was demonstrated that the hormones of the neural lobe of the rat hypophysis are located in separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres. These observations confirm the results of our previous immunocytochemical studies at the light microscopic level.", "PMID": 963734}
{"id": "pubmed23n0032_5673", "title": "Ultrastructural changes in spermatozoa of the brush-tailed possum, Trichosurus vulpecula (Marsupialia), during epididymal transit. Part II: The acrosome.", "content": "The acrosome in spermatozoa from the caput epididymidis of the Australian Brush-tailed possum, Trichosurus vulpecula, typically forms a cup-like structure, sitting on the anterior third of the dorsal surface of the nucleus. The base of the acrosomal 'cup' is narrowly separated from the nuclear surface, while the body of the 'cup' projects voluminously away from the nucleus. During epididymal transit these pronounced marginal extensions of the acrosome are retracted towards the nucleus, and the electron dense acrosomal material undergoes a process of compaction within the plasma membrane of the head to produce the convex ovate form of the definitive acrosome. During this process a variety of bizarre forms of the acrosome are produced before its final configuration is attained.", "contents": "Ultrastructural changes in spermatozoa of the brush-tailed possum, Trichosurus vulpecula (Marsupialia), during epididymal transit. Part II: The acrosome. The acrosome in spermatozoa from the caput epididymidis of the Australian Brush-tailed possum, Trichosurus vulpecula, typically forms a cup-like structure, sitting on the anterior third of the dorsal surface of the nucleus. The base of the acrosomal 'cup' is narrowly separated from the nuclear surface, while the body of the 'cup' projects voluminously away from the nucleus. During epididymal transit these pronounced marginal extensions of the acrosome are retracted towards the nucleus, and the electron dense acrosomal material undergoes a process of compaction within the plasma membrane of the head to produce the convex ovate form of the definitive acrosome. During this process a variety of bizarre forms of the acrosome are produced before its final configuration is attained.", "PMID": 963735}
{"id": "pubmed23n0032_5674", "title": "Spermiogenesis in the brush-tailed possum, Trichosurus vulpecula (Marsupialia). The development of the acrosome.", "content": "Acrosome development in the Australian Brush-tailed possum, Trichosurus vulpecula, displays a number of extraordinary features. This is particularly evident in the later stages of spermiogenesis, when the area of the nuclear surface bounded by the nuclear ring, and covered by the acrosome, is reduced considerable. As a result, the acrosomal material becomes located over its definitive position on the anterior third of the dorsal nuclear surface; in this process it is thrown into a series of folds, and a wide subacrosomal space is formed. Further changes around the time of spermiation result in the release of a spermatozoon in which a thin layer of acrosomal material is closely applied to the nucleus over the area of the definitive location of the acrosome, whilst its margins are greatly extended and project freely away from the nucleus. The latter feature does not appear to have been reported for the sperm of other mammals.", "contents": "Spermiogenesis in the brush-tailed possum, Trichosurus vulpecula (Marsupialia). The development of the acrosome. Acrosome development in the Australian Brush-tailed possum, Trichosurus vulpecula, displays a number of extraordinary features. This is particularly evident in the later stages of spermiogenesis, when the area of the nuclear surface bounded by the nuclear ring, and covered by the acrosome, is reduced considerable. As a result, the acrosomal material becomes located over its definitive position on the anterior third of the dorsal nuclear surface; in this process it is thrown into a series of folds, and a wide subacrosomal space is formed. Further changes around the time of spermiation result in the release of a spermatozoon in which a thin layer of acrosomal material is closely applied to the nucleus over the area of the definitive location of the acrosome, whilst its margins are greatly extended and project freely away from the nucleus. The latter feature does not appear to have been reported for the sperm of other mammals.", "PMID": 963736}
{"id": "pubmed23n0032_5675", "title": "Second-order ocellar neurons in the brain of the honeybee (Apis mellifera).", "content": "Electrophoretic injection of Procion Yellow M-R4 into the ocellar tract of the worker bee has revealed the following: Two types of giant axon run from the lateral ocellus to the circumesophageal neuropile, where one branches ipsilaterally and the other contralaterally. A third type comes from the median ocellus and can be traced into the cervical connectives. The largest dendritic complex is in the circumesophageal neuropile; in addition, fiber endings have been demonstrated in the following areas: in the subretinal region, along the optic commissure, in the medulla interna, in the subesophageal ganglion and between the neurosecretory cells of the pars intercerebralis. -- The giant fibers are enclosed in a glial sheath. Three types of cell body are described. One is associated with the glia; another, larger cell type comprises giant-axon somata. The third type of cell is small, and cannot yet be identified. Some of the histological results are discussed with respect to the possible function of the ocellus.", "contents": "Second-order ocellar neurons in the brain of the honeybee (Apis mellifera). Electrophoretic injection of Procion Yellow M-R4 into the ocellar tract of the worker bee has revealed the following: Two types of giant axon run from the lateral ocellus to the circumesophageal neuropile, where one branches ipsilaterally and the other contralaterally. A third type comes from the median ocellus and can be traced into the cervical connectives. The largest dendritic complex is in the circumesophageal neuropile; in addition, fiber endings have been demonstrated in the following areas: in the subretinal region, along the optic commissure, in the medulla interna, in the subesophageal ganglion and between the neurosecretory cells of the pars intercerebralis. -- The giant fibers are enclosed in a glial sheath. Three types of cell body are described. One is associated with the glia; another, larger cell type comprises giant-axon somata. The third type of cell is small, and cannot yet be identified. Some of the histological results are discussed with respect to the possible function of the ocellus.", "PMID": 963737}
{"id": "pubmed23n0032_5676", "title": "An electrogenic sodium pump and baroreceptor function in normotensive and spontaneously hypertensive rats.", "content": "Postexcitatory depression (PED) and adaptation were examined in slowly adapting aortic baroreceptors of normotensive rats (NTR) and spontaneously hypertensive rats (SHR); an aortic arch-aortic nerve preparation in vitro was used. PED was elicited either mechanically by employing single or double pressure steps, or electrically by antidromic stimulation of the aortic nerve. During PED the axon of the receptor was capable of conducting action potentials and the receptor itself could respond to increased pressures. The relationship between duration of PED and number of impulses preceding it was hyperbolic. In NTR's and SHR's PED was abolished by ouabain or solutions containing no potassium, neither of which affected the steady state pressure-volume relationship of the aorta. These interventions, which are known to block electrogenic pumps, also lowered the pressure threshold and increased the curvature of the steady state pressure-frequency curve. Furthermore, lithium, another agent that blocks electrogenic pumps, also abolished PED. Thus, PED is attributed mainly to an electrogenic sodium pump which operates normally in baroreceptors. We found that adaptation from peak transient to steady state frequency did not appear to be altered significantly when the pump was blocked. Blockage of the pump by ouabain is responsible for the baroreceptor reflex effects elicited by this drug. We conclude that resetting and reduced sensitivity in SHR baroreceptors are not attributed to significant differences in electrogenic pump activity.", "contents": "An electrogenic sodium pump and baroreceptor function in normotensive and spontaneously hypertensive rats. Postexcitatory depression (PED) and adaptation were examined in slowly adapting aortic baroreceptors of normotensive rats (NTR) and spontaneously hypertensive rats (SHR); an aortic arch-aortic nerve preparation in vitro was used. PED was elicited either mechanically by employing single or double pressure steps, or electrically by antidromic stimulation of the aortic nerve. During PED the axon of the receptor was capable of conducting action potentials and the receptor itself could respond to increased pressures. The relationship between duration of PED and number of impulses preceding it was hyperbolic. In NTR's and SHR's PED was abolished by ouabain or solutions containing no potassium, neither of which affected the steady state pressure-volume relationship of the aorta. These interventions, which are known to block electrogenic pumps, also lowered the pressure threshold and increased the curvature of the steady state pressure-frequency curve. Furthermore, lithium, another agent that blocks electrogenic pumps, also abolished PED. Thus, PED is attributed mainly to an electrogenic sodium pump which operates normally in baroreceptors. We found that adaptation from peak transient to steady state frequency did not appear to be altered significantly when the pump was blocked. Blockage of the pump by ouabain is responsible for the baroreceptor reflex effects elicited by this drug. We conclude that resetting and reduced sensitivity in SHR baroreceptors are not attributed to significant differences in electrogenic pump activity.", "PMID": 963833}
{"id": "pubmed23n0032_5677", "title": "Effect of variation in dietary NaCl intake on total and fractional renal blood flow in the normal and mercury-intoxicated rat.", "content": "We studied the effect of different chronic (3-4 weeks) dietary salt intakes on intrarenal hemodynamics of normal and mercury-intoxicated rats. Cardiac output (CO), total renal blood flow (RBF), and the zonal perfusion rate in the outer cortex (OC) and inner cortex (IC) were measured by the radioactive microsphere method. The distribution of cortical blood flow was calculated as the distribution index (DI), which reflects the ratio OC/IC. Rats were placed on a high salt diet (group I), intermediate salt diet (group II), or low salt diet (group III). For each group control rats (subgroup A) and mercury-intoxicated rats (subgroup B) were studied. No effect of the different salt intakes on the DI could be detected. The DI in group IA was 2.35 +/- 0.14; in IIA, 2.40 +/- 0.16; and in IIIA, 2.38 +/- 0.09 (P greater than 0.05). After mercury injection RBF changed from 5.32 +/- 0.36 ml/g.min(-1) (IIA) to 3.31 +/- 0.20 ml/g.min(-1), IIB and from 4.32 +/- 0.11ml/g.min(-1) (IIIA) to 1.98 +/- 0.10 ml/g.min(-1) (IIIB) P less than 0.01). The DI was lowered to 1.53 +/- 0.06 (IIB) (P less than 0.05) and to 1.16 +/- 0.10 (IIIB) (P less than 0.01). In both IIB and IIIB a marked elevation of the blood urea was noted (IIB = 97 +/- 9 MG/100 ML AND IIIB = 182 +/- 25 mg/100 ml). In group IB no effect on RBF, OC, IC, or DI could be observed (for all values, P greater than 0.05) despite similar histological renal lesions. Group IB rats also had normal blood urea levels (31 +/- 6 mg/100 ml;P greater than 0.05). We conclude (1) that variations in dietary salt intake appear to have no detectable effect on the intracortical blood flow distribution; and furthermore (2) that the mercury-induced acute renal failure (ARF) is characterized hemodynamically by a total renal and preferential outer cortical ischemia and that chronic salt loading prevents the ARF while preserving normal renal perfusion.", "contents": "Effect of variation in dietary NaCl intake on total and fractional renal blood flow in the normal and mercury-intoxicated rat. We studied the effect of different chronic (3-4 weeks) dietary salt intakes on intrarenal hemodynamics of normal and mercury-intoxicated rats. Cardiac output (CO), total renal blood flow (RBF), and the zonal perfusion rate in the outer cortex (OC) and inner cortex (IC) were measured by the radioactive microsphere method. The distribution of cortical blood flow was calculated as the distribution index (DI), which reflects the ratio OC/IC. Rats were placed on a high salt diet (group I), intermediate salt diet (group II), or low salt diet (group III). For each group control rats (subgroup A) and mercury-intoxicated rats (subgroup B) were studied. No effect of the different salt intakes on the DI could be detected. The DI in group IA was 2.35 +/- 0.14; in IIA, 2.40 +/- 0.16; and in IIIA, 2.38 +/- 0.09 (P greater than 0.05). After mercury injection RBF changed from 5.32 +/- 0.36 ml/g.min(-1) (IIA) to 3.31 +/- 0.20 ml/g.min(-1), IIB and from 4.32 +/- 0.11ml/g.min(-1) (IIIA) to 1.98 +/- 0.10 ml/g.min(-1) (IIIB) P less than 0.01). The DI was lowered to 1.53 +/- 0.06 (IIB) (P less than 0.05) and to 1.16 +/- 0.10 (IIIB) (P less than 0.01). In both IIB and IIIB a marked elevation of the blood urea was noted (IIB = 97 +/- 9 MG/100 ML AND IIIB = 182 +/- 25 mg/100 ml). In group IB no effect on RBF, OC, IC, or DI could be observed (for all values, P greater than 0.05) despite similar histological renal lesions. Group IB rats also had normal blood urea levels (31 +/- 6 mg/100 ml;P greater than 0.05). We conclude (1) that variations in dietary salt intake appear to have no detectable effect on the intracortical blood flow distribution; and furthermore (2) that the mercury-induced acute renal failure (ARF) is characterized hemodynamically by a total renal and preferential outer cortical ischemia and that chronic salt loading prevents the ARF while preserving normal renal perfusion.", "PMID": 963834}
{"id": "pubmed23n0032_5678", "title": "Effects of sodium pentobarbital anesthesia on left ventricular function and distribution of cardiac output in dogs, with particular reference to the mechanism for tachycardia.", "content": "Sodium pentobarbital (PB), 30 mg/kg, iv, was administered to 30 conscious dogs instrumented for measurement of cardiac output and regional blood flow distribution, left ventricular (LV) diameter, LV pressure, dP/dt, and dD/dt, i.e., velocity of myocardial fiber shortening. Ventilation was controlled during anesthesia to maintain arterial blood gases at control values for conscious dogs. The anesthetic produced an initial transient, peripheral vasodilation but the steady state effects 15-30 minutes later were characterized by slight reductions in mesenteric flow and cardiac output and increases in mesenteric and systemic resistances, whereas iliac and renal resistances were not significantly different from control. When heart rate rose, PB increased end-systolic diameter and decreased coronary resistance, LV end-diastolic diameter, dP/dt/P (42%), and shortening velocity (36%). When heart rate was controlled, PB still increased end-systolic diameter and decreased shortening velocity and dP/dt/P, as occurred during spontaneous rhythm, but end-diastolic diameter rose instead of falling and coronary resistance did not change. After recovery from bilateral cervical section of both carotid sinus and aortic nerves, PB failed to elicit tachycardia. Thus, PB affects systemic and regional hemodynamics only slightly, but depresses the myocardium markedly. The tachycardia associated with PB anesthesia in intact, trained dogs appears not to be only vagolytic, as previously thought, but is predominantly mediated through the arterial baroreceptor reflex.", "contents": "Effects of sodium pentobarbital anesthesia on left ventricular function and distribution of cardiac output in dogs, with particular reference to the mechanism for tachycardia. Sodium pentobarbital (PB), 30 mg/kg, iv, was administered to 30 conscious dogs instrumented for measurement of cardiac output and regional blood flow distribution, left ventricular (LV) diameter, LV pressure, dP/dt, and dD/dt, i.e., velocity of myocardial fiber shortening. Ventilation was controlled during anesthesia to maintain arterial blood gases at control values for conscious dogs. The anesthetic produced an initial transient, peripheral vasodilation but the steady state effects 15-30 minutes later were characterized by slight reductions in mesenteric flow and cardiac output and increases in mesenteric and systemic resistances, whereas iliac and renal resistances were not significantly different from control. When heart rate rose, PB increased end-systolic diameter and decreased coronary resistance, LV end-diastolic diameter, dP/dt/P (42%), and shortening velocity (36%). When heart rate was controlled, PB still increased end-systolic diameter and decreased shortening velocity and dP/dt/P, as occurred during spontaneous rhythm, but end-diastolic diameter rose instead of falling and coronary resistance did not change. After recovery from bilateral cervical section of both carotid sinus and aortic nerves, PB failed to elicit tachycardia. Thus, PB affects systemic and regional hemodynamics only slightly, but depresses the myocardium markedly. The tachycardia associated with PB anesthesia in intact, trained dogs appears not to be only vagolytic, as previously thought, but is predominantly mediated through the arterial baroreceptor reflex.", "PMID": 963835}
{"id": "pubmed23n0032_5679", "title": "Speech problems in a national survey: assessments and prevalences.", "content": "In this paper we present data on the prevalence of speech problems in a nationally representative group of children according to three modes of assessment. There was a large measure of disagreement between the assessments. Sex, social class, birth order and family size differences were examined. There was indirect evidence to suggest that the social class differences in the teacher's assessments of poor speech could be explained in terms of differences in children's dialect and language rather than a reflection of speech deficits. Finally we examined the relationship between parental assessment and the three modes of assessment. Overall, the degree of concurrence was low.", "contents": "Speech problems in a national survey: assessments and prevalences. In this paper we present data on the prevalence of speech problems in a nationally representative group of children according to three modes of assessment. There was a large measure of disagreement between the assessments. Sex, social class, birth order and family size differences were examined. There was indirect evidence to suggest that the social class differences in the teacher's assessments of poor speech could be explained in terms of differences in children's dialect and language rather than a reflection of speech deficits. Finally we examined the relationship between parental assessment and the three modes of assessment. Overall, the degree of concurrence was low.", "PMID": 963832}
{"id": "pubmed23n0032_5680", "title": "Bioassay in vivo for circulating vasoactive agents after renal artery constriction in dogs.", "content": "We used the gracilis muscle vascular bed to bioassay blood from the two renal veins, vena cava, and aorta continuously for the presence of vasoactive agents before and for 45 minutes after partial occlusion of the left renal artery in dogs. Compared to comparable blood samples from control dogs, left renal venous, vena caval, and aortic blood, but not right renal venous blood, from dogs with renal artery constriction developed vasoconstrictor activity. This was associated with increased renin concentration in plasma from the left renal vein and the vena cava and an increase in systemic arterial pressure. In dogs pretreated with indomethacin, blood from the right renal vein also showed vasoconstrictor activity. Pretreatment with antirenin serum abolished all of the differences between control and experimental dogs. These findings suggest that during acute unilateral renal artery constriction the constricted kidney releases renin and the contralateral kidney releases prostaglandins in sufficient quantity to produce systemic vascular effects.", "contents": "Bioassay in vivo for circulating vasoactive agents after renal artery constriction in dogs. We used the gracilis muscle vascular bed to bioassay blood from the two renal veins, vena cava, and aorta continuously for the presence of vasoactive agents before and for 45 minutes after partial occlusion of the left renal artery in dogs. Compared to comparable blood samples from control dogs, left renal venous, vena caval, and aortic blood, but not right renal venous blood, from dogs with renal artery constriction developed vasoconstrictor activity. This was associated with increased renin concentration in plasma from the left renal vein and the vena cava and an increase in systemic arterial pressure. In dogs pretreated with indomethacin, blood from the right renal vein also showed vasoconstrictor activity. Pretreatment with antirenin serum abolished all of the differences between control and experimental dogs. These findings suggest that during acute unilateral renal artery constriction the constricted kidney releases renin and the contralateral kidney releases prostaglandins in sufficient quantity to produce systemic vascular effects.", "PMID": 963836}
{"id": "pubmed23n0032_5681", "title": "beta-adrenergic blockade in essential hypertension: reduced renin release despite renal vasoconstriction.", "content": "The acute effects of small doses of intravenous propranolol on renin release and on circulatory dynamics were studied at the time of renal arteriography in 12 persons with essential hypertension. All of the subjects had a normal peripheral renin response to chronic sodium depletion and all had normal renal function. Seven subjects received a 10-mEq sodium diet. At the time of arteriography, arterial blood pressure, pulse rate, cardiac output, renal blood flow, and arterial and renal venous renin activity were measured before and 6-20 minutes after the intravenous administration of propranolol (9-18 mjg/kg). Average renin secretion rate in the salt-depleted subjects fell from 367 +/- 80 (SEM) U/ml per 100 g/min to 122 +/- 51 U/ml per 100 g (P=0.03) and renal plasma flow fell from 189 to 155 ml/min per 100 g (P = 0.018). We also found that in the salt-loaded subjects, renal plasma flow fell from 213 to 184 ml/min per 100 g (P = 0.025), whereas renin secretion did not change significantly in either group. We conclude that propranolol rapidly blocks renin release despite circulatory changes which ordinarily constitute a stimulus for renin secretion, i.e., renal vasoconstriction and reduced renal blood flow.", "contents": "beta-adrenergic blockade in essential hypertension: reduced renin release despite renal vasoconstriction. The acute effects of small doses of intravenous propranolol on renin release and on circulatory dynamics were studied at the time of renal arteriography in 12 persons with essential hypertension. All of the subjects had a normal peripheral renin response to chronic sodium depletion and all had normal renal function. Seven subjects received a 10-mEq sodium diet. At the time of arteriography, arterial blood pressure, pulse rate, cardiac output, renal blood flow, and arterial and renal venous renin activity were measured before and 6-20 minutes after the intravenous administration of propranolol (9-18 mjg/kg). Average renin secretion rate in the salt-depleted subjects fell from 367 +/- 80 (SEM) U/ml per 100 g/min to 122 +/- 51 U/ml per 100 g (P=0.03) and renal plasma flow fell from 189 to 155 ml/min per 100 g (P = 0.018). We also found that in the salt-loaded subjects, renal plasma flow fell from 213 to 184 ml/min per 100 g (P = 0.025), whereas renin secretion did not change significantly in either group. We conclude that propranolol rapidly blocks renin release despite circulatory changes which ordinarily constitute a stimulus for renin secretion, i.e., renal vasoconstriction and reduced renal blood flow.", "PMID": 963837}
{"id": "pubmed23n0032_5682", "title": "Gestational changes in pulmonary vascular responses in fetal lambs in utero.", "content": "Pulmonary arterial (PA) blood flow patterns, changes in pulmonary blood flow, and pulmonary vascular responses to graded hypoxemia and intravenous acetylcholine (ACh) were studied in 15 fetal lambs in utero 3-12 days after surgical implantation of an electromagnetic flow transducer and PA catheter. Phasic PA flow in the fetus was forward only during the first third of systole, almost zero during midsystole, and backward during late systole and early diastole. In contrast, neonatal lambs showed forward PA flow throughout systole. The constriction of the fetal pulmonary vasculature in response to progressive hypoxemia varied with gestational age. At 103 days there was no significant drop in PA flow and only a small increase in pulmonary vascular resistance (Rp) with hypoxemia. The greatest increase in Rp was seen in fetuses after 121 days of gestation. This response was unaffected by alpha- and beta-sympathetic and parasympathetic blockade. Similarly, the pulmonary vascular response to ACh injected into the fetal jugular vein depended on gestational age. Little or no increase in pulmonary flow was noted in the youngest fetus, whereas ACh produced a marked increase in pulmonary flow in festuses over 120 days of gestation. These data suggest that the mechanisms by which hypoxemia constricts and ACh relaxes the pulmonary vascular smooth muscle are not fully developed in fetal lambs at 100 days of gestation and furthermore, that these mechanisms progressively develop during the last third of gestation.", "contents": "Gestational changes in pulmonary vascular responses in fetal lambs in utero. Pulmonary arterial (PA) blood flow patterns, changes in pulmonary blood flow, and pulmonary vascular responses to graded hypoxemia and intravenous acetylcholine (ACh) were studied in 15 fetal lambs in utero 3-12 days after surgical implantation of an electromagnetic flow transducer and PA catheter. Phasic PA flow in the fetus was forward only during the first third of systole, almost zero during midsystole, and backward during late systole and early diastole. In contrast, neonatal lambs showed forward PA flow throughout systole. The constriction of the fetal pulmonary vasculature in response to progressive hypoxemia varied with gestational age. At 103 days there was no significant drop in PA flow and only a small increase in pulmonary vascular resistance (Rp) with hypoxemia. The greatest increase in Rp was seen in fetuses after 121 days of gestation. This response was unaffected by alpha- and beta-sympathetic and parasympathetic blockade. Similarly, the pulmonary vascular response to ACh injected into the fetal jugular vein depended on gestational age. Little or no increase in pulmonary flow was noted in the youngest fetus, whereas ACh produced a marked increase in pulmonary flow in festuses over 120 days of gestation. These data suggest that the mechanisms by which hypoxemia constricts and ACh relaxes the pulmonary vascular smooth muscle are not fully developed in fetal lambs at 100 days of gestation and furthermore, that these mechanisms progressively develop during the last third of gestation.", "PMID": 963838}
{"id": "pubmed23n0032_5683", "title": "Lower limit of cerebral blood flow autoregulation in experimental renovascular hypertension in the baboon.", "content": "The effect of chronic renovascular hypertension on the autoregulation of cerebral blood flow was studied in anesthetized baboons. Cerebral blood flow was measured by the intracarotid 133Xe clearance method. Six baboons with renal hypertension of 8-12 weeks' duration were compared with six normotensive controls. The lower limit of autoregulation was determined following controlled hemorrhage. In the initially normotensive baboons, cerebral blood flow remained constant until mean arterial pressure had decreased to the range of 45-59 mm Hg. Thereafter, cerebral blood flow decreased with each further decrease in mean arterial pressure. In the chronically hypertensive baboons cerebral blood flow autoregulated until the mean arterial pressure had decreased to the range of 75-89 mm Hg. Therefore, the lower limit of autoregulation of cerebral blood flow was shifted to higher absolute levels of mean arterial pressure in baboons with chronic renovascular hypertension presumably due to adaptive changes in the cerebral circulation.", "contents": "Lower limit of cerebral blood flow autoregulation in experimental renovascular hypertension in the baboon. The effect of chronic renovascular hypertension on the autoregulation of cerebral blood flow was studied in anesthetized baboons. Cerebral blood flow was measured by the intracarotid 133Xe clearance method. Six baboons with renal hypertension of 8-12 weeks' duration were compared with six normotensive controls. The lower limit of autoregulation was determined following controlled hemorrhage. In the initially normotensive baboons, cerebral blood flow remained constant until mean arterial pressure had decreased to the range of 45-59 mm Hg. Thereafter, cerebral blood flow decreased with each further decrease in mean arterial pressure. In the chronically hypertensive baboons cerebral blood flow autoregulated until the mean arterial pressure had decreased to the range of 75-89 mm Hg. Therefore, the lower limit of autoregulation of cerebral blood flow was shifted to higher absolute levels of mean arterial pressure in baboons with chronic renovascular hypertension presumably due to adaptive changes in the cerebral circulation.", "PMID": 963839}
{"id": "pubmed23n0032_5684", "title": "The response of canine coronary vascular resistance to local alterations in coronary arterial P CO2.", "content": "The effect of hypercapnia on coronary vascular resistance (CVR) was studied in seven open-chest dogs. Coronary blood flow was supplied to the cannulated left main coronary artery from the femoral artery by a precision pump. Coronary arterial PCO2 was locally controlled with a small membrane oxygenator in the coronary perfusion circuit. Each PCO2 change was made at a constant coronary flow, and CVR was calculated from the ratio of perfusion pressure to flow. Coronary sinus (CS) PCO2 and PO2 were recorded continuously from blood withdrawn through a CS catheter. Normocapnia (PCO2 = 42.3 +/- 2.8 mm Hg) was obtained with a membrane oxygenator gas composition of 95% O2-5% CO2, and hypocapnia was produced with 100% O2-0% CO2. In addition to physiology normal coronary flow (determined by a CS PO2 of 20-30 mm Hg) relatively high and low flow states were studied. At a normal control CS PO2, a decrease in coronary arterial PCO2 from 42.3 +/- 2.8 to 23.8 +/- 1.3 mm Hg caused CVR to increase by 84.2%, from 1.27 +/- 0.06 to 2.30 +/- 0.04 units. Since pH was inversely related to PCO2, the effect on CVR may have been mediated through a pH change. CS PCO2 decreased from 65.2 +/- 1.9 to 39.4 +/- 1.3 mm Hg. myocardial oxygen consumption was unchanged. Increases in CVR of 74.5, 119.5, and 69.3% occurred during hypocapnia in three additional experiments in which control arterial PO2 was maintained at 52-90 mm Hg. When CS PO2 was greater than 30 mm Hg, the normocapnic CVR was high, and was only minimally increased by hypocapnia. When coronary flow was reduced to an ischemic level there was little response in CVR to hypocapnia. Thus the level of arterial PCO2 can have an important effect on CVR independent of changes in O2 consumption. Myocardial PCO2, derived from metabolically produced CO2 and contributed to by arterial CO2, may be a major factor in normal control of coronary flow.", "contents": "The response of canine coronary vascular resistance to local alterations in coronary arterial P CO2. The effect of hypercapnia on coronary vascular resistance (CVR) was studied in seven open-chest dogs. Coronary blood flow was supplied to the cannulated left main coronary artery from the femoral artery by a precision pump. Coronary arterial PCO2 was locally controlled with a small membrane oxygenator in the coronary perfusion circuit. Each PCO2 change was made at a constant coronary flow, and CVR was calculated from the ratio of perfusion pressure to flow. Coronary sinus (CS) PCO2 and PO2 were recorded continuously from blood withdrawn through a CS catheter. Normocapnia (PCO2 = 42.3 +/- 2.8 mm Hg) was obtained with a membrane oxygenator gas composition of 95% O2-5% CO2, and hypocapnia was produced with 100% O2-0% CO2. In addition to physiology normal coronary flow (determined by a CS PO2 of 20-30 mm Hg) relatively high and low flow states were studied. At a normal control CS PO2, a decrease in coronary arterial PCO2 from 42.3 +/- 2.8 to 23.8 +/- 1.3 mm Hg caused CVR to increase by 84.2%, from 1.27 +/- 0.06 to 2.30 +/- 0.04 units. Since pH was inversely related to PCO2, the effect on CVR may have been mediated through a pH change. CS PCO2 decreased from 65.2 +/- 1.9 to 39.4 +/- 1.3 mm Hg. myocardial oxygen consumption was unchanged. Increases in CVR of 74.5, 119.5, and 69.3% occurred during hypocapnia in three additional experiments in which control arterial PO2 was maintained at 52-90 mm Hg. When CS PO2 was greater than 30 mm Hg, the normocapnic CVR was high, and was only minimally increased by hypocapnia. When coronary flow was reduced to an ischemic level there was little response in CVR to hypocapnia. Thus the level of arterial PCO2 can have an important effect on CVR independent of changes in O2 consumption. Myocardial PCO2, derived from metabolically produced CO2 and contributed to by arterial CO2, may be a major factor in normal control of coronary flow.", "PMID": 963840}
{"id": "pubmed23n0032_5685", "title": "The effect of prostaglandin A1 on renin and aldosterone in man.", "content": "Blood pressure, pulse rate, plasma aldosterone (PA), renin, and cortisol were monitored during graded intravenous infusions of prostaglandin At (PGA)1), 0.075-0.6 mug/kg min-1, alone and superimposed on angiotensin II (A II) administration in five normal men. The infusions of PGA1 did not affect blood pressure, but did progressively increase the pulse rate up to 15.2 +/- 2.0 (SEM- beats/min at the highest prostaglandin dose (0.6 mug/kg min-1). Both PA and plasma renin activity (PRA) increased in a dose-related fashion in response to the prostaglandin infusions. Aldosterone increased from a control of 4.8 +/- 0.4 to 20.7 +/- 1.2 ng and PRA increased from 0.9 +/- 0.1 to 5.4 +/- 0.4 ng/ml hr-1 at the dose of 0.6 mug/kg min-1. The correlation between the aldosterone and renin values was r = 0.85 P less than 0.001. In separate experiments, acute volume expansion with 2 liters of saline did not affect the increase in renin activity induced by exogenous prostaglandin. A II (5 ng/kg min-1) increased aldosterone and blood pressure and decreased the pulse rate. The hemodynamic effects were progressively reversed by the superimposed prostaglandin infusions, but the observed changes in renin and aldosterone concentrations were not further altered. The PA response to A II infusions was not influenced by indomethacin pretreatment. Prostaglandin A (infusion) appears to have a direct effect on renin release in man.", "contents": "The effect of prostaglandin A1 on renin and aldosterone in man. Blood pressure, pulse rate, plasma aldosterone (PA), renin, and cortisol were monitored during graded intravenous infusions of prostaglandin At (PGA)1), 0.075-0.6 mug/kg min-1, alone and superimposed on angiotensin II (A II) administration in five normal men. The infusions of PGA1 did not affect blood pressure, but did progressively increase the pulse rate up to 15.2 +/- 2.0 (SEM- beats/min at the highest prostaglandin dose (0.6 mug/kg min-1). Both PA and plasma renin activity (PRA) increased in a dose-related fashion in response to the prostaglandin infusions. Aldosterone increased from a control of 4.8 +/- 0.4 to 20.7 +/- 1.2 ng and PRA increased from 0.9 +/- 0.1 to 5.4 +/- 0.4 ng/ml hr-1 at the dose of 0.6 mug/kg min-1. The correlation between the aldosterone and renin values was r = 0.85 P less than 0.001. In separate experiments, acute volume expansion with 2 liters of saline did not affect the increase in renin activity induced by exogenous prostaglandin. A II (5 ng/kg min-1) increased aldosterone and blood pressure and decreased the pulse rate. The hemodynamic effects were progressively reversed by the superimposed prostaglandin infusions, but the observed changes in renin and aldosterone concentrations were not further altered. The PA response to A II infusions was not influenced by indomethacin pretreatment. Prostaglandin A (infusion) appears to have a direct effect on renin release in man.", "PMID": 963841}
{"id": "pubmed23n0032_5686", "title": "Morphology and relationship to extensibility curves of human mitral valve chordae tendineae.", "content": "Human mitral valve chordae tendineae in which elastic response curves are nonlinear have also been found to exhibit extensibility that increases with chordal size and decreases with chordal age. We used selective enzymatic digestion and scanning transmission electron microscopy to explain these observations. Removal of the outer elastin sheath by enzymatic digestion did not significantly affect the elastic response of this tissue. Scanning electron microscopy revealed that the collagen fibers in the central core of young chordae exhibited a very wavy pattern but the pattern in adult specimens was relatively straight. The increased waviness accounted for the greater extensibility of the young specimens. The collagen fibers from young and old chordae consisted of a network of collagen fibrils that became more collapsed when the tissue was fixed under tension. This network arrangement of the fibrils explains the nonlinearity in the elastic response of the tissue. Transmission electron micrographs showed that the density of collagen fibrils decreased as chordal size increased. The number of fibrils per 10(-8) cm2 of the central core decreased from 182.4 (SE = 1.3) to 131.3 (SE = 1.6) as average chordal cross-sectional area increased from 0.0016 cm2 to 0.0268 cm2. This difference in fibril density provides an explanation for the greater extensibility shown by the thicker chordae. The collagen fibril diameters ranged from 516 A to 552 A.", "contents": "Morphology and relationship to extensibility curves of human mitral valve chordae tendineae. Human mitral valve chordae tendineae in which elastic response curves are nonlinear have also been found to exhibit extensibility that increases with chordal size and decreases with chordal age. We used selective enzymatic digestion and scanning transmission electron microscopy to explain these observations. Removal of the outer elastin sheath by enzymatic digestion did not significantly affect the elastic response of this tissue. Scanning electron microscopy revealed that the collagen fibers in the central core of young chordae exhibited a very wavy pattern but the pattern in adult specimens was relatively straight. The increased waviness accounted for the greater extensibility of the young specimens. The collagen fibers from young and old chordae consisted of a network of collagen fibrils that became more collapsed when the tissue was fixed under tension. This network arrangement of the fibrils explains the nonlinearity in the elastic response of the tissue. Transmission electron micrographs showed that the density of collagen fibrils decreased as chordal size increased. The number of fibrils per 10(-8) cm2 of the central core decreased from 182.4 (SE = 1.3) to 131.3 (SE = 1.6) as average chordal cross-sectional area increased from 0.0016 cm2 to 0.0268 cm2. This difference in fibril density provides an explanation for the greater extensibility shown by the thicker chordae. The collagen fibril diameters ranged from 516 A to 552 A.", "PMID": 963842}
{"id": "pubmed23n0032_5687", "title": "Echocardiographic detection of intracardiac right-to-left shunts following peripheral vein injections.", "content": "A contrast echocardiographic technique using peripheral vein injections for the detection and localization of intracardiac right-to-left shunting is described. Fifty children underwent cardiac catheterization and peripheral contrast echocardiographic studies. The site, degree, and direction of shunting were established at catheterization by oximetry, indicator dilution analysis and/or angiography. Peripheral vein injections were performed from the right antecubital vein or the right saphenous vein. Right-to-left shunts were documented at catheterization in 27/50 patients; contrast echocardiographic studies indicated the presence and level of shunting in all 27 patients. The contrast echocardiographic technique using peripheral vein injections detects and localizes right-to-left intracardiac shunting. It is a safe and sensitive method to evaluate systemic desaturation in ambulatory and postoperative patients.", "contents": "Echocardiographic detection of intracardiac right-to-left shunts following peripheral vein injections. A contrast echocardiographic technique using peripheral vein injections for the detection and localization of intracardiac right-to-left shunting is described. Fifty children underwent cardiac catheterization and peripheral contrast echocardiographic studies. The site, degree, and direction of shunting were established at catheterization by oximetry, indicator dilution analysis and/or angiography. Peripheral vein injections were performed from the right antecubital vein or the right saphenous vein. Right-to-left shunts were documented at catheterization in 27/50 patients; contrast echocardiographic studies indicated the presence and level of shunting in all 27 patients. The contrast echocardiographic technique using peripheral vein injections detects and localizes right-to-left intracardiac shunting. It is a safe and sensitive method to evaluate systemic desaturation in ambulatory and postoperative patients.", "PMID": 963845}
{"id": "pubmed23n0032_5688", "title": "Echocardiographic features of congenital left ventricular inflow obstruction.", "content": "The echocardiographic features of congenital left ventricular inflow obstruction are described in six patients. The echocardiograms in two patients with cor triatriatum were distinguished by normal mitral valve motion and an abnormal echo within the left atrium. In two patients with supravalvar mitral ring, in addition to abnormal mitral valve motion, an abnormal echo, presumably originating from the obstructive membrane, was located between the anterior and posterior mitral leaflets. In two cases of parachute mitral valve, mitral valve motion was abnormal. In one of these cases there were multiple mitral valve echoes similar to those found in supravalvar mitral ring. The echocardiographic identification of an obstructive membrane within the left atrium is difficult because of the occurrence of artifacts. However, membranes may be identified if careful scanning techniques are employed in patients in whom left ventricular inflow obstruction is suspected. The echocardiogram is useful in detecting mitral valve abnormalities in these patients and is valuable in cases where mitral valve replacement is contemplated.", "contents": "Echocardiographic features of congenital left ventricular inflow obstruction. The echocardiographic features of congenital left ventricular inflow obstruction are described in six patients. The echocardiograms in two patients with cor triatriatum were distinguished by normal mitral valve motion and an abnormal echo within the left atrium. In two patients with supravalvar mitral ring, in addition to abnormal mitral valve motion, an abnormal echo, presumably originating from the obstructive membrane, was located between the anterior and posterior mitral leaflets. In two cases of parachute mitral valve, mitral valve motion was abnormal. In one of these cases there were multiple mitral valve echoes similar to those found in supravalvar mitral ring. The echocardiographic identification of an obstructive membrane within the left atrium is difficult because of the occurrence of artifacts. However, membranes may be identified if careful scanning techniques are employed in patients in whom left ventricular inflow obstruction is suspected. The echocardiogram is useful in detecting mitral valve abnormalities in these patients and is valuable in cases where mitral valve replacement is contemplated.", "PMID": 963846}
{"id": "pubmed23n0032_5689", "title": "Multiple accessory pathways in patients with the pre-excitation syndrome.", "content": "We have studied 135 patients with the pre-excitation syndrome and have demonstrated evidence of multiple accessory pathways in 20 patients. Five patients had two distinct accessory atrioventricular (A-V) connections, associated with enhanced A-V node conduction in one patient. Twelve patients had a single accessory A-V connection associated with enhanced A-V conduction. In one of these there was an additional fasciculo-ventricular connection. One patient had an accessory A-V connection associated with a nodoventricular bundle. Two patients had fasciculo-ventricular connections combined with enhanced A-V conduction. The latter two patients had electrocardiograms suggestive of a complete accessory A-V connection. Patients with enhanced A-V conduction had shorter cycle lengths during reciprocating tachycardia, primarily because of a short A-H during the dysrhythmia, than those without such conduction. In addition, patients with enhanced A-V conduction demonstrated more rapid conduction from atrium to His bundle during induced atrial fibrillation and two developed life-threatening ventricular responses during atrial fibrillation. A nodo-ventricular pathway was documented to participate in reciprocating tachycardia in one patient. Surgery was undertaken in 13 patients. In 11, the intraoperative mapping studies confirmed the preoperative predictions. In two patients, the presence of a second accessory A-V connection was documented after ablation of one.", "contents": "Multiple accessory pathways in patients with the pre-excitation syndrome. We have studied 135 patients with the pre-excitation syndrome and have demonstrated evidence of multiple accessory pathways in 20 patients. Five patients had two distinct accessory atrioventricular (A-V) connections, associated with enhanced A-V node conduction in one patient. Twelve patients had a single accessory A-V connection associated with enhanced A-V conduction. In one of these there was an additional fasciculo-ventricular connection. One patient had an accessory A-V connection associated with a nodoventricular bundle. Two patients had fasciculo-ventricular connections combined with enhanced A-V conduction. The latter two patients had electrocardiograms suggestive of a complete accessory A-V connection. Patients with enhanced A-V conduction had shorter cycle lengths during reciprocating tachycardia, primarily because of a short A-H during the dysrhythmia, than those without such conduction. In addition, patients with enhanced A-V conduction demonstrated more rapid conduction from atrium to His bundle during induced atrial fibrillation and two developed life-threatening ventricular responses during atrial fibrillation. A nodo-ventricular pathway was documented to participate in reciprocating tachycardia in one patient. Surgery was undertaken in 13 patients. In 11, the intraoperative mapping studies confirmed the preoperative predictions. In two patients, the presence of a second accessory A-V connection was documented after ablation of one.", "PMID": 963847}
{"id": "pubmed23n0032_5690", "title": "Use of changes in the epicardial QRS complex to assess interventions which modify the extent of myocardial necrosis following coronary artery occlusion.", "content": "The goal of this study was to determine if changes in the epicardial QRS complex after coronary artery occlusion (CAO) can be used to evaluate the efficacy of interventions designed to limit infarct size. Forty-one open-chest dogs with CAO were studied: 15 were controls, 18 received hyaluronidase and eight received propranolol starting 20 minutes after CAO. Epicardial ECGs were recorded at specific time intervals to analyze ST-segment elevation and changes in Q and R waves. Transmural specimens were obtained 24 hours after CAO from the same sites at which ECGs were recorded. Q wave development (deltaQ), R wave fall (deltaR), and their combination (deltaR + deltaQ) at 24 hours correlated with the extent of necrosis, as determined by myocardial creatine phosphokinase activity depression and histologic appearance. In the control group ST-segment elevation 15 minutes after CAO (ST15M) predicted changes in Q and R waves 24 hours later; in the treated groups, the same ST15M prior to drug administration resulted in significantly less QRS changes. Thus, 1) Q wave development and R wave fall 24 hours after CAO accurately reflect myocardial necrosis. 2) ST15M predicts subsequent changes in Q and R waves. 3) The efficacy of hyaluronidase and propranolol, agents previously shown to reduce myocardial necrosis, can be detected by less Q wave development and a smaller fall in R wave voltage.", "contents": "Use of changes in the epicardial QRS complex to assess interventions which modify the extent of myocardial necrosis following coronary artery occlusion. The goal of this study was to determine if changes in the epicardial QRS complex after coronary artery occlusion (CAO) can be used to evaluate the efficacy of interventions designed to limit infarct size. Forty-one open-chest dogs with CAO were studied: 15 were controls, 18 received hyaluronidase and eight received propranolol starting 20 minutes after CAO. Epicardial ECGs were recorded at specific time intervals to analyze ST-segment elevation and changes in Q and R waves. Transmural specimens were obtained 24 hours after CAO from the same sites at which ECGs were recorded. Q wave development (deltaQ), R wave fall (deltaR), and their combination (deltaR + deltaQ) at 24 hours correlated with the extent of necrosis, as determined by myocardial creatine phosphokinase activity depression and histologic appearance. In the control group ST-segment elevation 15 minutes after CAO (ST15M) predicted changes in Q and R waves 24 hours later; in the treated groups, the same ST15M prior to drug administration resulted in significantly less QRS changes. Thus, 1) Q wave development and R wave fall 24 hours after CAO accurately reflect myocardial necrosis. 2) ST15M predicts subsequent changes in Q and R waves. 3) The efficacy of hyaluronidase and propranolol, agents previously shown to reduce myocardial necrosis, can be detected by less Q wave development and a smaller fall in R wave voltage.", "PMID": 963848}
{"id": "pubmed23n0032_5691", "title": "The protective effect of hyperosmotic mannitol in myocardial ischemia and necrosis.", "content": "Morphologic and hemodynamic changes that occur following coronary occlusion are examined. The effectiveness of hyperosmotic mannitol in lessening the extent of myocardial damage is assessed and mechanisms for its action discussed. Forty and 60 min of coronary vascular occlusion followed by 15 and 45 min of reflow were associated with a persistence of ischemia following reflow of blood, as established by infusions of silastic into the aortic root. Electron microscopic studies demonstrated myocardial and endothelial cell swelling at the end of the reflow period. The process of cell swelling appeared to be initiated during the period of arterial occlusion. This cell swelling was reduced by elevation of serum osmolality by 30-40 mOsm above control with the administration of mannitol during and following occlusion. There was an associated 40-50% reduction of vascular resistance following occlusion if mannitol was administered. In addition, the extent of necrosis, which was widespread in untreated hearts 12 hours after occlusion, was strikingly less in the hearts of dogs which received mannitol. Thus, in ischemic myocardium, elevation of osmolality by mannitol reduces myocardial necrosis, probably through its restoration of normal cell volume.", "contents": "The protective effect of hyperosmotic mannitol in myocardial ischemia and necrosis. Morphologic and hemodynamic changes that occur following coronary occlusion are examined. The effectiveness of hyperosmotic mannitol in lessening the extent of myocardial damage is assessed and mechanisms for its action discussed. Forty and 60 min of coronary vascular occlusion followed by 15 and 45 min of reflow were associated with a persistence of ischemia following reflow of blood, as established by infusions of silastic into the aortic root. Electron microscopic studies demonstrated myocardial and endothelial cell swelling at the end of the reflow period. The process of cell swelling appeared to be initiated during the period of arterial occlusion. This cell swelling was reduced by elevation of serum osmolality by 30-40 mOsm above control with the administration of mannitol during and following occlusion. There was an associated 40-50% reduction of vascular resistance following occlusion if mannitol was administered. In addition, the extent of necrosis, which was widespread in untreated hearts 12 hours after occlusion, was strikingly less in the hearts of dogs which received mannitol. Thus, in ischemic myocardium, elevation of osmolality by mannitol reduces myocardial necrosis, probably through its restoration of normal cell volume.", "PMID": 963849}
{"id": "pubmed23n0032_5692", "title": "Regional contractility. Selective depression of ischemic myocardium by verapamil.", "content": "The effects of verapamil (0.02-0.2 mg/kg) on contractility in normal and partially ischemic myocardium were compared with the changes following propranolol (0.01-1.0 mg/kg). Regional contractile function was studied in open-chest dogs with ultrasonic crystals and ischemia was controlled by graded occlusion of a carotid-to-coronary artery shunt. Reduction in shunt perfusion pressure (40-55 mm Hg) resulted in hypokinesia. Verapamil depressed contractility in ischemic myocardium in 5/5 dogs, but did not alter the maximum velocity of shortening (max V) or end-diastolic segment length in normal myocardium. Propranolol in doses sufficient to depress ischemic myocardium also depressed contractile function in normal myocardium. In two dogs without coronary occlusion, verapamil (up to 1.0 mg/kg) increased end-diastolic segment length but did not reduce max V. We conclude that verapamil selectively depresses ischemic myocardium, a finding that may have clinical implication since ischemic injury can be decreased by reducing contractility (and thereby MVO2).", "contents": "Regional contractility. Selective depression of ischemic myocardium by verapamil. The effects of verapamil (0.02-0.2 mg/kg) on contractility in normal and partially ischemic myocardium were compared with the changes following propranolol (0.01-1.0 mg/kg). Regional contractile function was studied in open-chest dogs with ultrasonic crystals and ischemia was controlled by graded occlusion of a carotid-to-coronary artery shunt. Reduction in shunt perfusion pressure (40-55 mm Hg) resulted in hypokinesia. Verapamil depressed contractility in ischemic myocardium in 5/5 dogs, but did not alter the maximum velocity of shortening (max V) or end-diastolic segment length in normal myocardium. Propranolol in doses sufficient to depress ischemic myocardium also depressed contractile function in normal myocardium. In two dogs without coronary occlusion, verapamil (up to 1.0 mg/kg) increased end-diastolic segment length but did not reduce max V. We conclude that verapamil selectively depresses ischemic myocardium, a finding that may have clinical implication since ischemic injury can be decreased by reducing contractility (and thereby MVO2).", "PMID": 963850}
{"id": "pubmed23n0032_5693", "title": "Stress testing with ST-segment depression at rest. An angiographic correlation.", "content": "Near maximal graded exercise tests and coronary angiograms were compared in 37 patients with a history of chest pain and with ST segment depression at rest, who were free of obvious nonischemic causes of ST depression. Additional ST depression of 0.1 mV or more occurred with exercise in 26 patients and 23 of these had obstruction of one or more coronary arteries (sensitivity = 0.92). Eleven patients showed no additional ST-segment depression with exercise, and nine of these had normal coronary angiograms (specificity = 0.75). Patients with no increase in ST depression on exercise developed the highest heart rates; those with asymptomatic additional ST depression achieved intermediate rates; and those with anginal attacks during testing demonstrated the least heart rate acceleration. Those with less coronary obstruction exercised longer on the treadmill than those with more obstruction. Those showing added ST depression were predominantly men (18 of 26) and were older (mean 54 years) than those who did not (mean 44 years). No test complications were encountered. This study suggests that safe and effective stress testing may be accomplished not only in persons with normal resting ECGs but also in selected patients who have abnormal ST segments at rest.", "contents": "Stress testing with ST-segment depression at rest. An angiographic correlation. Near maximal graded exercise tests and coronary angiograms were compared in 37 patients with a history of chest pain and with ST segment depression at rest, who were free of obvious nonischemic causes of ST depression. Additional ST depression of 0.1 mV or more occurred with exercise in 26 patients and 23 of these had obstruction of one or more coronary arteries (sensitivity = 0.92). Eleven patients showed no additional ST-segment depression with exercise, and nine of these had normal coronary angiograms (specificity = 0.75). Patients with no increase in ST depression on exercise developed the highest heart rates; those with asymptomatic additional ST depression achieved intermediate rates; and those with anginal attacks during testing demonstrated the least heart rate acceleration. Those with less coronary obstruction exercised longer on the treadmill than those with more obstruction. Those showing added ST depression were predominantly men (18 of 26) and were older (mean 54 years) than those who did not (mean 44 years). No test complications were encountered. This study suggests that safe and effective stress testing may be accomplished not only in persons with normal resting ECGs but also in selected patients who have abnormal ST segments at rest.", "PMID": 963851}
{"id": "pubmed23n0032_5694", "title": "Limitations of thallium-201 myocardial perfusion scintigrams.", "content": "The reliability of myocardial perfusion scintigrams with thallium-201 (201Tl) for detecting areas of hypoperfusion was assessed in 16 closed-chest dogs. Variable areas of ischemia were produced either by occluding or stenosing the left anterior descending coronary artery. Cardiac scintigrams taken in four projections were compared with regional myocardial perfusion maps. Segmental concentrations and segmental perfusions were quantitated by counting the emissions from 201Tl and the microspheres in each of 96 segments of the left ventricle. In addition, studies with a phantom were performed. The results indicate: 1) The emissions from 201Tl and from microspheres correlated well in ischemic segments (r = 0.93 +/- SE 0.02). 2) Seven of twelve ischemic hearts had definitely abnormal scintigrams and in each of these the hypoperfused zone was greater than 4.9 grams and perfusion was decreased by more than 45%. 3) In the phantom, abnormal scintigrams could be detected in the presence of lesser deficits than in the dogs. The limitation of the thallium perfusion scintigrams will be the inconsistent detection of small perfusion deficits.", "contents": "Limitations of thallium-201 myocardial perfusion scintigrams. The reliability of myocardial perfusion scintigrams with thallium-201 (201Tl) for detecting areas of hypoperfusion was assessed in 16 closed-chest dogs. Variable areas of ischemia were produced either by occluding or stenosing the left anterior descending coronary artery. Cardiac scintigrams taken in four projections were compared with regional myocardial perfusion maps. Segmental concentrations and segmental perfusions were quantitated by counting the emissions from 201Tl and the microspheres in each of 96 segments of the left ventricle. In addition, studies with a phantom were performed. The results indicate: 1) The emissions from 201Tl and from microspheres correlated well in ischemic segments (r = 0.93 +/- SE 0.02). 2) Seven of twelve ischemic hearts had definitely abnormal scintigrams and in each of these the hypoperfused zone was greater than 4.9 grams and perfusion was decreased by more than 45%. 3) In the phantom, abnormal scintigrams could be detected in the presence of lesser deficits than in the dogs. The limitation of the thallium perfusion scintigrams will be the inconsistent detection of small perfusion deficits.", "PMID": 963852}
{"id": "pubmed23n0032_5695", "title": "Effect of coronary thrombus age on fibrinogen uptake.", "content": "A study was carried out to define the time limits during which an experimental coronary thrombus remains capable of incorporating fibrinogen. 131I-fibrinogen was given to intact anesthetized dogs at different time intervals, up to 67 hours, following the formation of a coronary thrombus by catheter-electrode. Radioactivity of the recovered thrombi as a whole and segmentally divided, was determined following variable time intervals of exposure to circulating fibrinogen and was expressed as thrombus/blood ratio. The results indicate that coronary thrombi formed in a normal coronary vessel remain capable of incorporating fibrinogen for at least eighteen hours, with no significant differences in the segmental distribution of radioactivity. These findings do not support the view that the recovery of isotopic fibrinogen, which was given after the onset of coronary symptoms, in thrombi from patients with myocardial infarction establishes that the thrombus was initiated after the ischemic process.", "contents": "Effect of coronary thrombus age on fibrinogen uptake. A study was carried out to define the time limits during which an experimental coronary thrombus remains capable of incorporating fibrinogen. 131I-fibrinogen was given to intact anesthetized dogs at different time intervals, up to 67 hours, following the formation of a coronary thrombus by catheter-electrode. Radioactivity of the recovered thrombi as a whole and segmentally divided, was determined following variable time intervals of exposure to circulating fibrinogen and was expressed as thrombus/blood ratio. The results indicate that coronary thrombi formed in a normal coronary vessel remain capable of incorporating fibrinogen for at least eighteen hours, with no significant differences in the segmental distribution of radioactivity. These findings do not support the view that the recovery of isotopic fibrinogen, which was given after the onset of coronary symptoms, in thrombi from patients with myocardial infarction establishes that the thrombus was initiated after the ischemic process.", "PMID": 963853}
{"id": "pubmed23n0032_5696", "title": "Continuous murmur following Mustard operation for transposition of the great arteries. A sign of pulmonary venous obstruction.", "content": "Three patients developed severe pulmonary venous obstruction following Mustard operation for transposition of the great arteries. Each patient had a soft continuous murmur with distinct diastolic accentuation at the low left sternal border or xiphoid area. Simultaneous recording of intracardiac sound and pressure in one patient showed that the murmur originated at the site of obstruction in the surgically-constructed pulmonary venous atrium. Selective cineangiograms demonstrated the baffle to be the cause of the obstruction. A continuous murmur in a patient following Mustard operation may suggest significant pulmonary venous obstruction.", "contents": "Continuous murmur following Mustard operation for transposition of the great arteries. A sign of pulmonary venous obstruction. Three patients developed severe pulmonary venous obstruction following Mustard operation for transposition of the great arteries. Each patient had a soft continuous murmur with distinct diastolic accentuation at the low left sternal border or xiphoid area. Simultaneous recording of intracardiac sound and pressure in one patient showed that the murmur originated at the site of obstruction in the surgically-constructed pulmonary venous atrium. Selective cineangiograms demonstrated the baffle to be the cause of the obstruction. A continuous murmur in a patient following Mustard operation may suggest significant pulmonary venous obstruction.", "PMID": 963854}
{"id": "pubmed23n0032_5697", "title": "De subitaneis mortibus. XX. Cardiac electrical instability in the presence of a left superior vena cava.", "content": "Abnormalities of cardiac rhythm have been reported by others in patients with a persistent left superior vena cava. We present the histological findings from postmortem examination of the cardiac conduction system of two such patients. The first patient was a schoolboy who died suddenly and unexpectedly. His sinus node was abnormally small, his A-V node contained numerous venous lacunae and was stretched out beneath the enlarged coronary sinus, both A-V node and His bundle were dispersed in the central fibrous body in a fetal pattern, and isolated fragments of A-V nodal tissue were connected to the myocardium at the crest of the interventricular septum. The second patient complained of palpitations many months prior to surgical correction of an interventricular septal defect; his postoperative course included multiple arrhythmias and he died suddenly on the 16th postoperative day. Although his sinus node was histologically normal, the nutrient artery contained a polypoid fibromuscular  mass virtually occluding its lumen; his A-V node and His bundle were also dispersed in the central fibrous body in the fetal pattern, and the A-V node contained numerous venous lacunae as well as being histologically disorganized in its cytological pattern. These anatomical findings may indicate a basis for various forms of cardiac electrical instability, and deserve consideration in the future evaluation of any patient found to have a persistent left superior vene cava, but particularly if there is clinical evidence suggesting an arrhythmia or conduction disturbance.", "contents": "De subitaneis mortibus. XX. Cardiac electrical instability in the presence of a left superior vena cava. Abnormalities of cardiac rhythm have been reported by others in patients with a persistent left superior vena cava. We present the histological findings from postmortem examination of the cardiac conduction system of two such patients. The first patient was a schoolboy who died suddenly and unexpectedly. His sinus node was abnormally small, his A-V node contained numerous venous lacunae and was stretched out beneath the enlarged coronary sinus, both A-V node and His bundle were dispersed in the central fibrous body in a fetal pattern, and isolated fragments of A-V nodal tissue were connected to the myocardium at the crest of the interventricular septum. The second patient complained of palpitations many months prior to surgical correction of an interventricular septal defect; his postoperative course included multiple arrhythmias and he died suddenly on the 16th postoperative day. Although his sinus node was histologically normal, the nutrient artery contained a polypoid fibromuscular  mass virtually occluding its lumen; his A-V node and His bundle were also dispersed in the central fibrous body in the fetal pattern, and the A-V node contained numerous venous lacunae as well as being histologically disorganized in its cytological pattern. These anatomical findings may indicate a basis for various forms of cardiac electrical instability, and deserve consideration in the future evaluation of any patient found to have a persistent left superior vene cava, but particularly if there is clinical evidence suggesting an arrhythmia or conduction disturbance.", "PMID": 963855}
{"id": "pubmed23n0032_5698", "title": "Predisposing factors and the development of reaginic allergy in infancy.", "content": "In a prospective study of fifty-eight newborn infants of parents with reaginic allergy, twenty-seven developed eczema and twenty-eight positive prick tests to one or more of six antigens during the first year of life. These reaginic manifestations were related to presymptomatic transient IgA deficiency. The development of positive skin tests was also related to HLA A1 B8, and to season of birth. The order of frequency of positivity was Dermatophagoides, grass pollens, cat fur, feathers and cow's milk. The skin tests were often positive in infants in whom serum IgE was not detected. The eczema disappeared and the skin tests became negative in some infants at the end of the first year. This work suggests that sensitization in the new-born period is important in the subsequent development of disease.", "contents": "Predisposing factors and the development of reaginic allergy in infancy. In a prospective study of fifty-eight newborn infants of parents with reaginic allergy, twenty-seven developed eczema and twenty-eight positive prick tests to one or more of six antigens during the first year of life. These reaginic manifestations were related to presymptomatic transient IgA deficiency. The development of positive skin tests was also related to HLA A1 B8, and to season of birth. The order of frequency of positivity was Dermatophagoides, grass pollens, cat fur, feathers and cow's milk. The skin tests were often positive in infants in whom serum IgE was not detected. The eczema disappeared and the skin tests became negative in some infants at the end of the first year. This work suggests that sensitization in the new-born period is important in the subsequent development of disease.", "PMID": 963861}
{"id": "pubmed23n0032_5699", "title": "Immunological development in infants of allergic parents.", "content": "Serum immunoglobulins were determined every 4 months from cord blood to age 2 years in ninety-two infants of allergic parents. By 7 months of age, the concentration of IgA was significantly greater in infants who developed atopic dermatitis than those who did not. The IgA was also significantly greater than in matched non-allergic controls. The difference continued to the end of the 2-year study. No significant difference was found in the three groups for levels of IgG and IgM. These findings differ from the results of a previous report and possible explanations are presented.", "contents": "Immunological development in infants of allergic parents. Serum immunoglobulins were determined every 4 months from cord blood to age 2 years in ninety-two infants of allergic parents. By 7 months of age, the concentration of IgA was significantly greater in infants who developed atopic dermatitis than those who did not. The IgA was also significantly greater than in matched non-allergic controls. The difference continued to the end of the 2-year study. No significant difference was found in the three groups for levels of IgG and IgM. These findings differ from the results of a previous report and possible explanations are presented.", "PMID": 963862}
{"id": "pubmed23n0032_5700", "title": "Asthma and wheezy bronchitis in children. Skin test reactivity in cases, their parents and siblings. A controlled population study of sex differences.", "content": "Twice as many boys as girls are prone to wheeze (15-3% compared with 7%). This study examines the possible immunological basis for this phenomenon. There were 1064 children in this survey. The family of each wheezy child was indexed, and a control sample of families was selected. At least 85% of the study population were skin tested for Type 1 allergic reactions. An interval survey of values for FEV1, FVC and PEFR against height was completed. Of 118 wheezy subjects there were 83/542 boys and 35/522 girls. Single and multiple sensitivity, the capacity to react to two or more major allergens, is examined. Of the child asthmatics, significant differences from controls emerge for pollens, foods and the house dust mite. These differences are borne out with multiple sensitivity. (P greater than 0-001.) Of the brothers and sisters of asthmatics, only the sisters show differences from controls. This is true for grass pollen, cow's milk and the house dust mite, and also to a highly significant degree for multiple sensitivity (P greater than 0-001). When the incidence of multiple sensitivity is expressed for all children in the asthma group, that is, cases and their siblings, the sex difference is not demonstrable. The mothers (P greater than 0-05) and fathers (P greater than 0-01), of asthmatics also differ from control parents in respect of skin sensitivity. If multiple sensitivity is equated with atopy, it may not be the main determination of the capacity to wheeze, and thus of the sex difference. The mean curve for interval PEFR for asthmatic children was significantly lower than that of control children and of the brothers and sisters of asthmatics.", "contents": "Asthma and wheezy bronchitis in children. Skin test reactivity in cases, their parents and siblings. A controlled population study of sex differences. Twice as many boys as girls are prone to wheeze (15-3% compared with 7%). This study examines the possible immunological basis for this phenomenon. There were 1064 children in this survey. The family of each wheezy child was indexed, and a control sample of families was selected. At least 85% of the study population were skin tested for Type 1 allergic reactions. An interval survey of values for FEV1, FVC and PEFR against height was completed. Of 118 wheezy subjects there were 83/542 boys and 35/522 girls. Single and multiple sensitivity, the capacity to react to two or more major allergens, is examined. Of the child asthmatics, significant differences from controls emerge for pollens, foods and the house dust mite. These differences are borne out with multiple sensitivity. (P greater than 0-001.) Of the brothers and sisters of asthmatics, only the sisters show differences from controls. This is true for grass pollen, cow's milk and the house dust mite, and also to a highly significant degree for multiple sensitivity (P greater than 0-001). When the incidence of multiple sensitivity is expressed for all children in the asthma group, that is, cases and their siblings, the sex difference is not demonstrable. The mothers (P greater than 0-05) and fathers (P greater than 0-01), of asthmatics also differ from control parents in respect of skin sensitivity. If multiple sensitivity is equated with atopy, it may not be the main determination of the capacity to wheeze, and thus of the sex difference. The mean curve for interval PEFR for asthmatic children was significantly lower than that of control children and of the brothers and sisters of asthmatics.", "PMID": 963863}
{"id": "pubmed23n0032_5701", "title": "Demonstration of reaginic antibodies on human basophils by immune adherence to allergen-coated Sepharose beads.", "content": "Human basophils purified by gradient density centrifugation and differential glass bead adherence were interacted with grass pollen allergen in particulate form prepared by covalent coupling of Dactylis glomerata protein concentrate to Sepharose beads. Basophils from allergic subjects were found to interact specifically with the allergen-coated bead surface in a manner so highly characteristic that false negatives with basophils from non-allergic subjects or subjects with unrelated allergies were not encountered. Microscopic examination indicated that the specifically stained malleable basophils had adapted in a multi-point attachment to the rigid bead surface by becoming one-sidedly flattened against it. When using basophils from highly pollen sensitive subjects all the beads carried basophils and sometimes as many as thirty per bead. The percentage of beads with basophils and the number of basophils per bead roughly correlated with clinical history and skin tests. Immunocytoadherence of the basophils to the allergen-coated beads was specifically inhibited by anti-IgE and anti-allergen antibodies, including reagins.", "contents": "Demonstration of reaginic antibodies on human basophils by immune adherence to allergen-coated Sepharose beads. Human basophils purified by gradient density centrifugation and differential glass bead adherence were interacted with grass pollen allergen in particulate form prepared by covalent coupling of Dactylis glomerata protein concentrate to Sepharose beads. Basophils from allergic subjects were found to interact specifically with the allergen-coated bead surface in a manner so highly characteristic that false negatives with basophils from non-allergic subjects or subjects with unrelated allergies were not encountered. Microscopic examination indicated that the specifically stained malleable basophils had adapted in a multi-point attachment to the rigid bead surface by becoming one-sidedly flattened against it. When using basophils from highly pollen sensitive subjects all the beads carried basophils and sometimes as many as thirty per bead. The percentage of beads with basophils and the number of basophils per bead roughly correlated with clinical history and skin tests. Immunocytoadherence of the basophils to the allergen-coated beads was specifically inhibited by anti-IgE and anti-allergen antibodies, including reagins.", "PMID": 963864}
{"id": "pubmed23n0032_5702", "title": "Intranasal steroid aerosol in perennial rhinitis: comparison with an antihistamine compound.", "content": "Intranasal betamethasone valerate aerosol, given for 28 days, was compared with an oral antihistamine compound in a couble-blind, double-dummy, cross-over trial involving thirty patients with perennial rhinitis. The steroid aerosol was more effective in reducing symptoms and was preferred by the patients (P less than 0-01). Nasal blockage index, calculated from oral and nasal peak expiratory flow measurements, did not provide useful or additional information. There were no side effects from the steroid and Candida albicans was not cultured from nasal swabs. It is concluded that beta-methasone valerate aerosol is a suitable short-term alternative for patients whose perennial rhinitis fails to respond to conventional therapy.", "contents": "Intranasal steroid aerosol in perennial rhinitis: comparison with an antihistamine compound. Intranasal betamethasone valerate aerosol, given for 28 days, was compared with an oral antihistamine compound in a couble-blind, double-dummy, cross-over trial involving thirty patients with perennial rhinitis. The steroid aerosol was more effective in reducing symptoms and was preferred by the patients (P less than 0-01). Nasal blockage index, calculated from oral and nasal peak expiratory flow measurements, did not provide useful or additional information. There were no side effects from the steroid and Candida albicans was not cultured from nasal swabs. It is concluded that beta-methasone valerate aerosol is a suitable short-term alternative for patients whose perennial rhinitis fails to respond to conventional therapy.", "PMID": 963865}
{"id": "pubmed23n0032_5703", "title": "The relationship between bronchial histamine reactivity and atopic status.", "content": "It has been proposed that increased production of IgE, a feature of atopy, is a cause of the non-specific bronchial hyper-reactivity that is characterisitc of asthma. This hypothesis was examined by selecting groups of subjects with asthma or rhinitis and a group of healthy control subjects and studying the relationship between their bronchial histamine reactivity and their atopic status. In none of the groups tested was there a significant association between the degree of bronchial histamine reactivity and either the serum level of total IgE or the number of extracts of aeroallergens giving positive prick test reactions.", "contents": "The relationship between bronchial histamine reactivity and atopic status. It has been proposed that increased production of IgE, a feature of atopy, is a cause of the non-specific bronchial hyper-reactivity that is characterisitc of asthma. This hypothesis was examined by selecting groups of subjects with asthma or rhinitis and a group of healthy control subjects and studying the relationship between their bronchial histamine reactivity and their atopic status. In none of the groups tested was there a significant association between the degree of bronchial histamine reactivity and either the serum level of total IgE or the number of extracts of aeroallergens giving positive prick test reactions.", "PMID": 963866}
{"id": "pubmed23n0032_5704", "title": "Qualitative and quantitative comparison of pollen calendars for plain and mountain areas.", "content": "A comparison of the quantity and the quality of pollen content in the atmosphere of two regions, one of the plains bordering the Mediterranean sea (altitude 40 m), the other of the mountains of the Pyrenees (altitude 1550 m), was made during the climatological year 1974-1975. The method which was used intercepts the pollen flux of the atmosphere with a vertical filtering unit, which is exposed facing the direction of wind on the filtering door of the weather-vane and collects much larger quantities of pollen than the other techniques of collection. The atmospheric currents displace from one region to another numerous spores and pollens, the trajectories of which are directly influenced by the general circulation of the atmosphere. The density of these fluxes can be very large, reaching 2500 grains/m3 of air at M.T.P.R. in the last week of January; the taxons collected are very numerous (more than 700). The atmospheric transfer of pollen modifies largely the pollen content of the atmosphere of different flowering domains. These interferences manifest themselves in the simplest cases by the presence of two peaks of concentration, the principal corresponding to the local efflorescence, the second to the pollen transferred from other regions. The pollen concentrations in the mountains are one-third of those in the plains. Pollination in the mountains is several weeks late in comparison to the plain during the first semester, whereas during the second semester, it is the plains that show a certain delay. These findings show why some allergic patients who go to the mountains during summer are affected twice by pollination.", "contents": "Qualitative and quantitative comparison of pollen calendars for plain and mountain areas. A comparison of the quantity and the quality of pollen content in the atmosphere of two regions, one of the plains bordering the Mediterranean sea (altitude 40 m), the other of the mountains of the Pyrenees (altitude 1550 m), was made during the climatological year 1974-1975. The method which was used intercepts the pollen flux of the atmosphere with a vertical filtering unit, which is exposed facing the direction of wind on the filtering door of the weather-vane and collects much larger quantities of pollen than the other techniques of collection. The atmospheric currents displace from one region to another numerous spores and pollens, the trajectories of which are directly influenced by the general circulation of the atmosphere. The density of these fluxes can be very large, reaching 2500 grains/m3 of air at M.T.P.R. in the last week of January; the taxons collected are very numerous (more than 700). The atmospheric transfer of pollen modifies largely the pollen content of the atmosphere of different flowering domains. These interferences manifest themselves in the simplest cases by the presence of two peaks of concentration, the principal corresponding to the local efflorescence, the second to the pollen transferred from other regions. The pollen concentrations in the mountains are one-third of those in the plains. Pollination in the mountains is several weeks late in comparison to the plain during the first semester, whereas during the second semester, it is the plains that show a certain delay. These findings show why some allergic patients who go to the mountains during summer are affected twice by pollination.", "PMID": 963867}
{"id": "pubmed23n0032_5705", "title": "Identification of allergen-specific IgE antibodies in the sera of African asthmatics.", "content": "Of eighty African asthmatics, sixty-three had significant levels of specific IgE against the house dust mite dermatophagoides pteronyssinus. By contrast, grass-specific IgE was found in six patients and mould-specific IgE in none. Sixty-three patients also had symptoms predominantly during the rainy season. Positive skin tests against D. pteronyssinus correlated well with the presence of mite-specific IgE, as did the size of these skin test weals with the amount of specific IgE. For grass pollen and moulds there was no such relationship. There was no correlation between a history of sensitivity to house dust and either skin tests or specific IgE against D. pteronyssinus. The results support previous findings that it is allergy to house dust mite and not to grass pollens or moulds which is important in producing the seasonal symptoms in our patients.", "contents": "Identification of allergen-specific IgE antibodies in the sera of African asthmatics. Of eighty African asthmatics, sixty-three had significant levels of specific IgE against the house dust mite dermatophagoides pteronyssinus. By contrast, grass-specific IgE was found in six patients and mould-specific IgE in none. Sixty-three patients also had symptoms predominantly during the rainy season. Positive skin tests against D. pteronyssinus correlated well with the presence of mite-specific IgE, as did the size of these skin test weals with the amount of specific IgE. For grass pollen and moulds there was no such relationship. There was no correlation between a history of sensitivity to house dust and either skin tests or specific IgE against D. pteronyssinus. The results support previous findings that it is allergy to house dust mite and not to grass pollens or moulds which is important in producing the seasonal symptoms in our patients.", "PMID": 963868}
{"id": "pubmed23n0032_5706", "title": "Preanesthetic care. Intoxication and trauma.", "content": "On the basis of the above discussion, a number of useful guidelines appear for the anesthetic management of alcohol and drug abusers. 1. Because of the decreased ability of intoxicated patients to withstand hemorrhage, blood replacement therapy should probably be instituted earlier than in the nonintoxicated patient. 2. Because the chronic alcoholic may actually be iso-osmotically overhydrated, fluid therapy must be planned with care. 3. Because of the tendency to hypoglycemia, glucose should be added to the fluid management regimen. 4. Because of the enzyme induction effect of chronic ETOH ingestion, anesthetic agents that are in part metabolized (methoxyflurane, halothane, fluroxene) are perhaps best avoided. Increased ability to metabolize anesthetic agents appears to be associated with toxicity. 5. Because ETOH is a CNS depressant and has been shown to have amnesia-inducing properties, supplementation of nitrous oxide-relaxant technique with narcotics or other depressant drugs should be reduced, if not eliminated. 6. Because acutely intoxicated individuals are more prone to hypothermia, their core temperature should be monitored intraoperatively. All intravenous fluids should be warmed and a warming blanket should be employed, if necessary, to maintain body temperature. 7. Because of the sympathomimetic effect of many of the drugs, pulse and blood pressure can be misleading in the assessment of blood loss.", "contents": "Preanesthetic care. Intoxication and trauma. On the basis of the above discussion, a number of useful guidelines appear for the anesthetic management of alcohol and drug abusers. 1. Because of the decreased ability of intoxicated patients to withstand hemorrhage, blood replacement therapy should probably be instituted earlier than in the nonintoxicated patient. 2. Because the chronic alcoholic may actually be iso-osmotically overhydrated, fluid therapy must be planned with care. 3. Because of the tendency to hypoglycemia, glucose should be added to the fluid management regimen. 4. Because of the enzyme induction effect of chronic ETOH ingestion, anesthetic agents that are in part metabolized (methoxyflurane, halothane, fluroxene) are perhaps best avoided. Increased ability to metabolize anesthetic agents appears to be associated with toxicity. 5. Because ETOH is a CNS depressant and has been shown to have amnesia-inducing properties, supplementation of nitrous oxide-relaxant technique with narcotics or other depressant drugs should be reduced, if not eliminated. 6. Because acutely intoxicated individuals are more prone to hypothermia, their core temperature should be monitored intraoperatively. All intravenous fluids should be warmed and a warming blanket should be employed, if necessary, to maintain body temperature. 7. Because of the sympathomimetic effect of many of the drugs, pulse and blood pressure can be misleading in the assessment of blood loss.", "PMID": 963873}
{"id": "pubmed23n0032_5707", "title": "Transportation and emergency care. Emergency assessment and management.", "content": "An organized approach for the management of the multiply injured patient has been presented. Adequate equipment and ancillary facilities support patient care. The principles of airway management, treatment of shock, and specific organ injuries have been described. More than one surgical discipline may work on a patient simultaneously; however, one physician, preferably the trauma surgeon or general surgeon, is in charge and coordinates the consultative care.", "contents": "Transportation and emergency care. Emergency assessment and management. An organized approach for the management of the multiply injured patient has been presented. Adequate equipment and ancillary facilities support patient care. The principles of airway management, treatment of shock, and specific organ injuries have been described. More than one surgical discipline may work on a patient simultaneously; however, one physician, preferably the trauma surgeon or general surgeon, is in charge and coordinates the consultative care.", "PMID": 963876}
{"id": "pubmed23n0032_5708", "title": "Postanesthetic care. Respiratory care.", "content": "Careful management of all severely patients, traumatized particularly those undergoing general anesthesia and surgery, is essential to prevent the development of serious pulmonary problems in the postoperative period. In addition to insuring adequate oxygenation, humidification, and analgesia, particular attention should be paid to careful monitoring of volume therapy, efficient filtration of banked blood, and the prevention of aspiration.", "contents": "Postanesthetic care. Respiratory care. Careful management of all severely patients, traumatized particularly those undergoing general anesthesia and surgery, is essential to prevent the development of serious pulmonary problems in the postoperative period. In addition to insuring adequate oxygenation, humidification, and analgesia, particular attention should be paid to careful monitoring of volume therapy, efficient filtration of banked blood, and the prevention of aspiration.", "PMID": 963879}
{"id": "pubmed23n0032_5709", "title": "The interactions between iophenoxic acid, iopanoic acid, bilirubin and human serum albumin as studied by fluorescence and Sephadex gel filtration.", "content": "Iophenoxic acid increases the fluorescence of bilirubin bound to human serum albumin at drug/albumin molar ratios lower than 1, while iopanoic acid decreases it. The fluorescence enhancement results probably from a change in the fluorescence efficiency due to an iophenoxic acid-induced conformational change in the albumin, which in turn causes displacement of bilirubin from the protein. Iophenoxic acid does not affect the high-affinity bilirubin binding site of albumin. Therefore any enhancement in bilirubin fluorescence caused by the drug indicates that bilirubin is bound to the low-affinity binding sites of albumin. The use of iophenoxic acid in the determination of the extent of saturation of the high-affinity bilirubin binding site of albumin may be of value in the clinical management of infants with neonatal jaundice.", "contents": "The interactions between iophenoxic acid, iopanoic acid, bilirubin and human serum albumin as studied by fluorescence and Sephadex gel filtration. Iophenoxic acid increases the fluorescence of bilirubin bound to human serum albumin at drug/albumin molar ratios lower than 1, while iopanoic acid decreases it. The fluorescence enhancement results probably from a change in the fluorescence efficiency due to an iophenoxic acid-induced conformational change in the albumin, which in turn causes displacement of bilirubin from the protein. Iophenoxic acid does not affect the high-affinity bilirubin binding site of albumin. Therefore any enhancement in bilirubin fluorescence caused by the drug indicates that bilirubin is bound to the low-affinity binding sites of albumin. The use of iophenoxic acid in the determination of the extent of saturation of the high-affinity bilirubin binding site of albumin may be of value in the clinical management of infants with neonatal jaundice.", "PMID": 963887}
{"id": "pubmed23n0032_5710", "title": "Mammalian acetoacetate decarboxylase activity. Its distribution in subfractions of human albumin and occurrence in various tissues of the rat.", "content": "In this article further information is presented about the characteristics of the mammalian enzyme acetoacetate decarboxylase (acetoacetate carboxylase, EC 4.1.1.4). The Michaelis-Menten plot shows a sigmoidal relationship between the enzyme activity (v) and the substrate concentration (s) indicating an allosteric hindrance. Because of this, the KM value can only be predicted to be equal to or less than 1 X 10(-1) M. Cysteine and glutathione, although activating the spontaneous decarboxylation, have no effect upon the enzyme activity. From experiments with human albumin by means of gel filtration with Sephadex G-200, it can be concluded that the acetoacetate decarboxylase activity does not depend upon the degree of polymerisation of albumin. From experiments performed by means of ion exchange chromatography the enzyme activity may be localized in the non-mercaptalbumin fraction. Investigation of enzyme activity in homogenates of various rat tissues, as well as in their respective subfractions, reveals that: (1) the specific activity of brain tissue exceeds those of liver and kidney and (2) most of the activity in liver tissue is localized in the 20 000 X g supernatant, containing the endoplasmatic reticulum, the ribosomes and the soluble part of the cytoplasm, while in brain tissue a high activity is found in the nuclei fraction.", "contents": "Mammalian acetoacetate decarboxylase activity. Its distribution in subfractions of human albumin and occurrence in various tissues of the rat. In this article further information is presented about the characteristics of the mammalian enzyme acetoacetate decarboxylase (acetoacetate carboxylase, EC 4.1.1.4). The Michaelis-Menten plot shows a sigmoidal relationship between the enzyme activity (v) and the substrate concentration (s) indicating an allosteric hindrance. Because of this, the KM value can only be predicted to be equal to or less than 1 X 10(-1) M. Cysteine and glutathione, although activating the spontaneous decarboxylation, have no effect upon the enzyme activity. From experiments with human albumin by means of gel filtration with Sephadex G-200, it can be concluded that the acetoacetate decarboxylase activity does not depend upon the degree of polymerisation of albumin. From experiments performed by means of ion exchange chromatography the enzyme activity may be localized in the non-mercaptalbumin fraction. Investigation of enzyme activity in homogenates of various rat tissues, as well as in their respective subfractions, reveals that: (1) the specific activity of brain tissue exceeds those of liver and kidney and (2) most of the activity in liver tissue is localized in the 20 000 X g supernatant, containing the endoplasmatic reticulum, the ribosomes and the soluble part of the cytoplasm, while in brain tissue a high activity is found in the nuclei fraction.", "PMID": 963888}
{"id": "pubmed23n0032_5711", "title": "Determination of serum triglycerides by mass fragmentography.", "content": "A mass fragmentographic reference method for determination of serum triglycerides is described. A fixed amount of a mixture of [1,1,2,3,3-2H5]glycerol tripalmitate and [1,1,2,3,3-2H5]glycerol trioleate (500 nmol) is added to a fixed amount of serum (250 mul) and extracted with chloroform/methanol (2 : 1, v/v). The triglycerides are isolated by means of thin-layer chromatography. The glycerol obtained after acid hydrolysis is converted into the tri-trimethylsilyl derivative and the amount of unlabeled glycerol is determined from the ratio between the recordings at m/e 218 m/e 222, obtained after analysis with a gas chromatograph-mass spectrometer equipped with an MID (multiple ion detector). The two ions correspond to the peak at M--90 and M--91 in the mass spectrum of the tri-trimethylsilyl derivative of unlabeled and (1,1,2,3,3-2H5)-labeled glycerol. The relative standard deviation of the method in the range 0.4--5.8 mmol/l was 2.4%. A fully enzymatic routine method for determination triglycerides was compared with the mass fragmentographic reference method. There was a good correlation between the two methods (r = 0.995) and the regression coefficient was 1.19.", "contents": "Determination of serum triglycerides by mass fragmentography. A mass fragmentographic reference method for determination of serum triglycerides is described. A fixed amount of a mixture of [1,1,2,3,3-2H5]glycerol tripalmitate and [1,1,2,3,3-2H5]glycerol trioleate (500 nmol) is added to a fixed amount of serum (250 mul) and extracted with chloroform/methanol (2 : 1, v/v). The triglycerides are isolated by means of thin-layer chromatography. The glycerol obtained after acid hydrolysis is converted into the tri-trimethylsilyl derivative and the amount of unlabeled glycerol is determined from the ratio between the recordings at m/e 218 m/e 222, obtained after analysis with a gas chromatograph-mass spectrometer equipped with an MID (multiple ion detector). The two ions correspond to the peak at M--90 and M--91 in the mass spectrum of the tri-trimethylsilyl derivative of unlabeled and (1,1,2,3,3-2H5)-labeled glycerol. The relative standard deviation of the method in the range 0.4--5.8 mmol/l was 2.4%. A fully enzymatic routine method for determination triglycerides was compared with the mass fragmentographic reference method. There was a good correlation between the two methods (r = 0.995) and the regression coefficient was 1.19.", "PMID": 963889}
{"id": "pubmed23n0032_5712", "title": "The relationship between serum carnitine levels and the nutritional status of patients with schistosomiasis.", "content": "Serum carnitine levels were investigated in a group of normal adults and two groups of patients with active schistosomiasis who also showed signs of malnutrition and vitamin deficiency. The first group consisted of 16 patients with Schistosoma mansoni and/or Schistosoma haematobium infection. They received an adequate diet supplemented with vitamin and iron therapy and received no treatment for their parasitic infection till their hemoglobin levels were restored to normal. The second group consisted of 12 patients with schistosomiasis as well as intestinal polyposis. They received the same diet as the first group but because of their poor condition were immediately treated for parasitic infection. Results showed that both groups of patients had subnormal levels of serum carnitine with the polyps patients (Group II) having a significantly lower level than patients with simple schistosomiasis (Group I). After nutritional repletion a significant increase was observed in the carnitine levels of most patients in group I indicating a relationship between the nutritional status of the patients and their serum carnitine levels. The patients with polyps also showed considerably increased carnitine levels after treatment and dietary repletion. The usefulness of serum carnitine measurement as an index of protein malnutrition in man is discussed.", "contents": "The relationship between serum carnitine levels and the nutritional status of patients with schistosomiasis. Serum carnitine levels were investigated in a group of normal adults and two groups of patients with active schistosomiasis who also showed signs of malnutrition and vitamin deficiency. The first group consisted of 16 patients with Schistosoma mansoni and/or Schistosoma haematobium infection. They received an adequate diet supplemented with vitamin and iron therapy and received no treatment for their parasitic infection till their hemoglobin levels were restored to normal. The second group consisted of 12 patients with schistosomiasis as well as intestinal polyposis. They received the same diet as the first group but because of their poor condition were immediately treated for parasitic infection. Results showed that both groups of patients had subnormal levels of serum carnitine with the polyps patients (Group II) having a significantly lower level than patients with simple schistosomiasis (Group I). After nutritional repletion a significant increase was observed in the carnitine levels of most patients in group I indicating a relationship between the nutritional status of the patients and their serum carnitine levels. The patients with polyps also showed considerably increased carnitine levels after treatment and dietary repletion. The usefulness of serum carnitine measurement as an index of protein malnutrition in man is discussed.", "PMID": 963890}
{"id": "pubmed23n0032_5713", "title": "Lysosomal enzymes in cerebral atrophy.", "content": "In seven patients with cerebral atrophy due to pre-senile dementia and/or cerebrovascular disease, the activity of acid phosphatase in lumbar cerebrospinal fluid (CSF) was higher (p less than 0.05) than in six controls. The activity of arylsulphatase and beta-galactosidase in CSF was the same in the two groups. In the serum, the activities of acid phosphatase and arylsulphatase were the same in the two groups but the activity of beta-galactosidase was lower (p less than 0.02) in patients with cerebral atrophy.", "contents": "Lysosomal enzymes in cerebral atrophy. In seven patients with cerebral atrophy due to pre-senile dementia and/or cerebrovascular disease, the activity of acid phosphatase in lumbar cerebrospinal fluid (CSF) was higher (p less than 0.05) than in six controls. The activity of arylsulphatase and beta-galactosidase in CSF was the same in the two groups. In the serum, the activities of acid phosphatase and arylsulphatase were the same in the two groups but the activity of beta-galactosidase was lower (p less than 0.02) in patients with cerebral atrophy.", "PMID": 963891}
{"id": "pubmed23n0032_5714", "title": "A method for the statistical evaluation of results in external quality control surveys.", "content": "A method for the evaluation of results in external quality control surveys is developed on purely statistical grounds. This system allows to establish accuracy and precision of assay methods by comparing statistical data from subsequent surveys. However, for a particular survey the overall accuracy, precision and quality of each laboratory are defined in statistical terms and visually displayed. Results for each laboratory are summarized on a two-page computer printout. Per component assayed and combining all components determined in the particular survey, the laboratory is assigned one of the four possible qualifications i.e. \"very good\", \"moderate\" or \"bad\". A few general conclusions drawn from experimental data of previous surveys are reported.", "contents": "A method for the statistical evaluation of results in external quality control surveys. A method for the evaluation of results in external quality control surveys is developed on purely statistical grounds. This system allows to establish accuracy and precision of assay methods by comparing statistical data from subsequent surveys. However, for a particular survey the overall accuracy, precision and quality of each laboratory are defined in statistical terms and visually displayed. Results for each laboratory are summarized on a two-page computer printout. Per component assayed and combining all components determined in the particular survey, the laboratory is assigned one of the four possible qualifications i.e. \"very good\", \"moderate\" or \"bad\". A few general conclusions drawn from experimental data of previous surveys are reported.", "PMID": 963892}
{"id": "pubmed23n0032_5715", "title": "Automated determination of the antitrypsin activity of serum.", "content": "An automated determination of the serum antitrypsin activity is described in which all reaction parameters have been optimized. The determination is carried out by measuring the difference between the activity of a standard amount of trypsin with and without addition of serum. Trypsin activity is measured using N-alpha-benzoyl-L-arginine-p-nitroanilide as a substrate. The assay is performed by means of an automatic enzyme reaction rate analyser the Vitatron AKES. The antitrypsin activity is expressed in Inhibitor Units making standardization of the trypsin preparation superfluous. Normal values of the antitrypsin activity for some of the most frequently occurring genetic variants of alpha1-antitrypsin are given.", "contents": "Automated determination of the antitrypsin activity of serum. An automated determination of the serum antitrypsin activity is described in which all reaction parameters have been optimized. The determination is carried out by measuring the difference between the activity of a standard amount of trypsin with and without addition of serum. Trypsin activity is measured using N-alpha-benzoyl-L-arginine-p-nitroanilide as a substrate. The assay is performed by means of an automatic enzyme reaction rate analyser the Vitatron AKES. The antitrypsin activity is expressed in Inhibitor Units making standardization of the trypsin preparation superfluous. Normal values of the antitrypsin activity for some of the most frequently occurring genetic variants of alpha1-antitrypsin are given.", "PMID": 963893}
{"id": "pubmed23n0032_5716", "title": "An active site peptide from human placental alkaline phosphatase.", "content": "A preparation of human placental alkaline phosphatase was labelled covalently at the active site with [32P]orthophosphate. Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight. Dansylation of the peptide showed that the amino-terminal residue was glycine. After acid hydrolysis the only radioactively-labelled amino acid present was serine phosphate. The amino acid composition was similar to those compositions reported for active site peptides from other alkaline phosphatases.", "contents": "An active site peptide from human placental alkaline phosphatase. A preparation of human placental alkaline phosphatase was labelled covalently at the active site with [32P]orthophosphate. Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight. Dansylation of the peptide showed that the amino-terminal residue was glycine. After acid hydrolysis the only radioactively-labelled amino acid present was serine phosphate. The amino acid composition was similar to those compositions reported for active site peptides from other alkaline phosphatases.", "PMID": 963894}
{"id": "pubmed23n0032_5717", "title": "The determination of progesterone receptors in breast cancer and their relationship to estrogen receptors.", "content": "A simple method for the assay of specific progesterone receptors in breast cancer tissue is described. Progesterone receptors were detected in 63 of 74 breast cancer specimens (85%). Estrogen receptor positive tumors had a wide range of progesterone receptor concentrations, but in 77% of cases the level was above 3 fmol/mg protein. The progesterone receptor level was generally low in tumors lacking estrogen receptors, 75% of the samples having concentrations between 0 and 3 fmol/mg protein. Unlike estrogen receptors, age had no influence on the number of progesterone receptors in breast cancer tissue.", "contents": "The determination of progesterone receptors in breast cancer and their relationship to estrogen receptors. A simple method for the assay of specific progesterone receptors in breast cancer tissue is described. Progesterone receptors were detected in 63 of 74 breast cancer specimens (85%). Estrogen receptor positive tumors had a wide range of progesterone receptor concentrations, but in 77% of cases the level was above 3 fmol/mg protein. The progesterone receptor level was generally low in tumors lacking estrogen receptors, 75% of the samples having concentrations between 0 and 3 fmol/mg protein. Unlike estrogen receptors, age had no influence on the number of progesterone receptors in breast cancer tissue.", "PMID": 963895}
{"id": "pubmed23n0032_5718", "title": "Oxalic acid determination in plasma.", "content": "A method is described for the estimation of oxalic acid in plasma. After removing interfering substances with an ion-exchange resin, oxalic acid is reduced to glycolic acid, which forms a coloured compound with chromotropic acid. No special equipment is required. A good recovery and precision are found.", "contents": "Oxalic acid determination in plasma. A method is described for the estimation of oxalic acid in plasma. After removing interfering substances with an ion-exchange resin, oxalic acid is reduced to glycolic acid, which forms a coloured compound with chromotropic acid. No special equipment is required. A good recovery and precision are found.", "PMID": 963896}
{"id": "pubmed23n0032_5719", "title": "Effect of riboflavin treatment on human red cell pyruvate kinase deficiency.", "content": "The effect of riboflavin on six patients with hemolytic anemia due to hereditary pyruvate kinase deficiency was studied. Treatment over a period of four to six months produced no significant hematologic or enzymologic changes in the variants investigated.", "contents": "Effect of riboflavin treatment on human red cell pyruvate kinase deficiency. The effect of riboflavin on six patients with hemolytic anemia due to hereditary pyruvate kinase deficiency was studied. Treatment over a period of four to six months produced no significant hematologic or enzymologic changes in the variants investigated.", "PMID": 963898}
{"id": "pubmed23n0032_5720", "title": "Excretion of neuraminic acid-containing trisaccharides in the urine during pregnancy.", "content": "The excretion of neuraminic acid-containing trisaccharides in the urine during the course of normal pregnancy was studied. Neuraminyl-lactose and neuraminyl-galactosyl-(l led to 4)-N-acetylglucosamine were identified, and their excretion patterns were analyzed by thin-layer and gas chromatography. A progressive increase in the outputs of these acidic oligosaccharides was observed during the course of pregnancy in all cases studied. Neuraminyl-lactose excretion increased threefold, from 13.7 +/- 1.75 to 37.1 +/- 2.56 mg/24 h, and neuraminyl-galactosyl-N-acetylglucosamine twofold, from 8.4 +/- 1.27 to 15.3 +/- 2.21 mg/24 h (Mean +/- S.E.).", "contents": "Excretion of neuraminic acid-containing trisaccharides in the urine during pregnancy. The excretion of neuraminic acid-containing trisaccharides in the urine during the course of normal pregnancy was studied. Neuraminyl-lactose and neuraminyl-galactosyl-(l led to 4)-N-acetylglucosamine were identified, and their excretion patterns were analyzed by thin-layer and gas chromatography. A progressive increase in the outputs of these acidic oligosaccharides was observed during the course of pregnancy in all cases studied. Neuraminyl-lactose excretion increased threefold, from 13.7 +/- 1.75 to 37.1 +/- 2.56 mg/24 h, and neuraminyl-galactosyl-N-acetylglucosamine twofold, from 8.4 +/- 1.27 to 15.3 +/- 2.21 mg/24 h (Mean +/- S.E.).", "PMID": 963899}
{"id": "pubmed23n0032_5721", "title": "3-Hydroxy-3-methylglutaric aciduria: deficiency of 3-hydroxy-3-methylglutaryl coenzyme A lyase.", "content": "A marked deficiency of 3-hydroxy-3-methylglutaryl coenzyme A lyase activity is present in cultured skin fibroblasts from a baby with 3-hydroxy-3-methylglutaric aciduria.", "contents": "3-Hydroxy-3-methylglutaric aciduria: deficiency of 3-hydroxy-3-methylglutaryl coenzyme A lyase. A marked deficiency of 3-hydroxy-3-methylglutaryl coenzyme A lyase activity is present in cultured skin fibroblasts from a baby with 3-hydroxy-3-methylglutaric aciduria.", "PMID": 963901}
{"id": "pubmed23n0032_5722", "title": "A screening test for patients suspected of having Turner syndrome.", "content": "An index has been devised using dermatoglyphics and selected physical traits to screen  for patients suspected of having Turner syndrome. About 60% of females with and without Turner syndrome can be diagnosed as having or not having the syndrome with a 98% or greater probability. The patient's score on the index, expressed in probability, can be used to decide whether chromosome studies should be done. Using the approach demonstrated in this pilot study, the discriminative power can be increased by adding more features and by enlarging the sample to permit division of features into more discriminating classes.", "contents": "A screening test for patients suspected of having Turner syndrome. An index has been devised using dermatoglyphics and selected physical traits to screen  for patients suspected of having Turner syndrome. About 60% of females with and without Turner syndrome can be diagnosed as having or not having the syndrome with a 98% or greater probability. The patient's score on the index, expressed in probability, can be used to decide whether chromosome studies should be done. Using the approach demonstrated in this pilot study, the discriminative power can be increased by adding more features and by enlarging the sample to permit division of features into more discriminating classes.", "PMID": 963904}
{"id": "pubmed23n0032_5723", "title": "Origin of the extra chromosome in trisomy 16.", "content": "Chromosome analysis was carried out on 22 spontaneous abortuses with trisomy 16 and their parents by means of sequential Q- and C-banding techniques. In seven cases, the extra chromosome No. 16 originated from a non-disjunctional error in the first meiotic division in the mother, and in two cases from an error in the first meiotic division in the father. In two cases, non-disjunction had occurred during the second meiotic division (one in the mother and one in the father). It seems that trisomy 16, although independent of maternal age, most frequently results from a first meiotic non-disjunction in the mother.", "contents": "Origin of the extra chromosome in trisomy 16. Chromosome analysis was carried out on 22 spontaneous abortuses with trisomy 16 and their parents by means of sequential Q- and C-banding techniques. In seven cases, the extra chromosome No. 16 originated from a non-disjunctional error in the first meiotic division in the mother, and in two cases from an error in the first meiotic division in the father. In two cases, non-disjunction had occurred during the second meiotic division (one in the mother and one in the father). It seems that trisomy 16, although independent of maternal age, most frequently results from a first meiotic non-disjunction in the mother.", "PMID": 963905}
{"id": "pubmed23n0032_5724", "title": "Genetic risks in Perthes' disease.", "content": "A population study of Perthes' disease in South Wales over a 25-year period has shown a low risk of the disorder in relatives. The risk of Perthes' disease in sibs is under 1% (2 in 323), while the risk to children of an children of an affected parent is about 3% (1 in 35) when adjusted for age of onset. No increased risk was found in relatives of patients with bilateral as compared with unilateral Perthes' disease. A mating between two affected individuals resulted in monozygotic twins concordant for Perthes' disease, but no familial concentration to suggest the action of a single major gene was found. The importance of excluding generalised disorders which may mimic Perthes' disease and may show Mendelian inheritance is emphasised, but it is concluded that true Perthes' disease has a relatively minor genetic component.", "contents": "Genetic risks in Perthes' disease. A population study of Perthes' disease in South Wales over a 25-year period has shown a low risk of the disorder in relatives. The risk of Perthes' disease in sibs is under 1% (2 in 323), while the risk to children of an children of an affected parent is about 3% (1 in 35) when adjusted for age of onset. No increased risk was found in relatives of patients with bilateral as compared with unilateral Perthes' disease. A mating between two affected individuals resulted in monozygotic twins concordant for Perthes' disease, but no familial concentration to suggest the action of a single major gene was found. The importance of excluding generalised disorders which may mimic Perthes' disease and may show Mendelian inheritance is emphasised, but it is concluded that true Perthes' disease has a relatively minor genetic component.", "PMID": 963906}
{"id": "pubmed23n0032_5725", "title": "Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material.", "content": "Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-insoluble radioactivity increased lineraly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.", "contents": "Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material. Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-insoluble radioactivity increased lineraly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.", "PMID": 963907}
{"id": "pubmed23n0032_5726", "title": "Surface receptors on human haematopoietic cell lines.", "content": "The expression of complement receptors, of Fc receptors, of SRBC receptors and of S-Ig was investigated on human haematopoietic cell lines of proved malignant derivation. According to their origin and to a panel of phenotypic markers these lines have been classified into lymphoma lines, myeloma lines and leukemia lines. Results were compared with those obtained on non-malignant EBV carrying lymphoblastoid cell lines (LCL). Among the lymphoid cell lines the LCL showed a pattern of B-lymphocyte surface markers, i.e. surface immunoglobulins, C3 receptors but low density of Fc receptors. The non-Burkitt lymphoma lines bore in varying degree these B-lymphocyte markers. The lines U-698 M and DG-75 were exceptional in having only surface immunoglobulin. The Burkitt lymphoma lines had all B-lymphocyte markers. The myeloma lines differed from the lymphoid lines in lacking C3 and Fc receptors and showed only trace amounts of surface immunoglobulins. In contrast to lymphoid and myeloma lines, the leukaemia lines were completely lacking surface immunoglobulins, but showed C3 and Fc receptors in variable densities. On line, the ALL derived line MOLT-3 showed the capacity to spontaneous rosette formation with SRBC. The findings that LCL presented a homogeneous pattern of B-lymphocyte surface markers may be of value in order to discriminate between these lines and lines derived from haematopoietic malignancies other than Burkitt lymphomas.", "contents": "Surface receptors on human haematopoietic cell lines. The expression of complement receptors, of Fc receptors, of SRBC receptors and of S-Ig was investigated on human haematopoietic cell lines of proved malignant derivation. According to their origin and to a panel of phenotypic markers these lines have been classified into lymphoma lines, myeloma lines and leukemia lines. Results were compared with those obtained on non-malignant EBV carrying lymphoblastoid cell lines (LCL). Among the lymphoid cell lines the LCL showed a pattern of B-lymphocyte surface markers, i.e. surface immunoglobulins, C3 receptors but low density of Fc receptors. The non-Burkitt lymphoma lines bore in varying degree these B-lymphocyte markers. The lines U-698 M and DG-75 were exceptional in having only surface immunoglobulin. The Burkitt lymphoma lines had all B-lymphocyte markers. The myeloma lines differed from the lymphoid lines in lacking C3 and Fc receptors and showed only trace amounts of surface immunoglobulins. In contrast to lymphoid and myeloma lines, the leukaemia lines were completely lacking surface immunoglobulins, but showed C3 and Fc receptors in variable densities. On line, the ALL derived line MOLT-3 showed the capacity to spontaneous rosette formation with SRBC. The findings that LCL presented a homogeneous pattern of B-lymphocyte surface markers may be of value in order to discriminate between these lines and lines derived from haematopoietic malignancies other than Burkitt lymphomas.", "PMID": 963908}
{"id": "pubmed23n0032_5727", "title": "Differences between plasma and serum complement in patients with chronic liver disease.", "content": "Of sixty patients with chronic liver disease, eight with cirrhosis or chronic hepatitis had very low serum CH50 but normal plasma CH50. The complement component profiles of these sera revealed markedly decreased C4 and C2 activities and normal C3T (C3-C9) activities. From these results, it is suggested that the early acting complement components had been non-specifically activated during blood coagulation in these patients. No difference between plasma and serum CH50 was found in patients with hepatitis B(s) antigen.", "contents": "Differences between plasma and serum complement in patients with chronic liver disease. Of sixty patients with chronic liver disease, eight with cirrhosis or chronic hepatitis had very low serum CH50 but normal plasma CH50. The complement component profiles of these sera revealed markedly decreased C4 and C2 activities and normal C3T (C3-C9) activities. From these results, it is suggested that the early acting complement components had been non-specifically activated during blood coagulation in these patients. No difference between plasma and serum CH50 was found in patients with hepatitis B(s) antigen.", "PMID": 963909}
{"id": "pubmed23n0032_5728", "title": "A new haemolytic assay for the second component of complement (C2) in human serum.", "content": "A new, specific haemolytic assay for C2 is described using EAC43 (Antrypol) cells and serum deficient in C4 and C2 from a patient with hereditary angio-oedema. The method is simple and the results in pathological and normal sera correlate well with those obtained using the EA and C2-deficient serum method.", "contents": "A new haemolytic assay for the second component of complement (C2) in human serum. A new, specific haemolytic assay for C2 is described using EAC43 (Antrypol) cells and serum deficient in C4 and C2 from a patient with hereditary angio-oedema. The method is simple and the results in pathological and normal sera correlate well with those obtained using the EA and C2-deficient serum method.", "PMID": 963910}
{"id": "pubmed23n0032_5729", "title": "Hapten-specific lymphocyte transformation in humans sensitized with NDMA or DNCB.", "content": "The primary immune response to a contact sensitizing dose of para-N-dimethylnitrosaniline (NDMA) and dinitrochlorobenzene (DNCB) was obtained in humans and measured in vitro by increased thymidine incorporation into sensitized lymphocytes. No cross-reaction was found between these two haptens, and it is thus possible on two separate occasions to quantify and follow the primary cellular immune response in man.", "contents": "Hapten-specific lymphocyte transformation in humans sensitized with NDMA or DNCB. The primary immune response to a contact sensitizing dose of para-N-dimethylnitrosaniline (NDMA) and dinitrochlorobenzene (DNCB) was obtained in humans and measured in vitro by increased thymidine incorporation into sensitized lymphocytes. No cross-reaction was found between these two haptens, and it is thus possible on two separate occasions to quantify and follow the primary cellular immune response in man.", "PMID": 963911}
{"id": "pubmed23n0032_5730", "title": "Regionalization of perinatal care for the United States.", "content": "A scheme of regionalization is proposed in which a regional perinatal system within a defined geographic area permits access of the high risk mother-fetus and infant to the appropriate level of technology for improved perinatal outcome.", "contents": "Regionalization of perinatal care for the United States. A scheme of regionalization is proposed in which a regional perinatal system within a defined geographic area permits access of the high risk mother-fetus and infant to the appropriate level of technology for improved perinatal outcome.", "PMID": 963920}
{"id": "pubmed23n0032_5731", "title": "The perinatal research program of the National Institute of Child Health and Human Development.", "content": "The Institute's research emphasis is on the interrelationships of specific health and developmental problems in the antenatal, perinatal, and infant periods, and the effects that these events may have upon maternal health, maternal-infant relationships, and subsequent development and well being of the child.", "contents": "The perinatal research program of the National Institute of Child Health and Human Development. The Institute's research emphasis is on the interrelationships of specific health and developmental problems in the antenatal, perinatal, and infant periods, and the effects that these events may have upon maternal health, maternal-infant relationships, and subsequent development and well being of the child.", "PMID": 963921}
{"id": "pubmed23n0032_5732", "title": "Newborn Country USA.", "content": "\"Newborn Country USA\" describes a concept for a regionwide, integrated perinatal care system that serves a population of more than four million. How the four sectors of the Denver Regional Perinatal Center - The Children's Hospital, Denver, Denver General Hospital, Fitzsimons Army Medical Center, and the University of Colorado Army Medical Center - interact as a regional resource, relate to 223 hospitals in portions of 10 states, and assist in serving a portion of 70,000 live births in 1976 are reported.", "contents": "Newborn Country USA. \"Newborn Country USA\" describes a concept for a regionwide, integrated perinatal care system that serves a population of more than four million. How the four sectors of the Denver Regional Perinatal Center - The Children's Hospital, Denver, Denver General Hospital, Fitzsimons Army Medical Center, and the University of Colorado Army Medical Center - interact as a regional resource, relate to 223 hospitals in portions of 10 states, and assist in serving a portion of 70,000 live births in 1976 are reported.", "PMID": 963922}
{"id": "pubmed23n0032_5733", "title": "Neonatal intensive care in community hospitals and remote areas. The problems and a possible solution.", "content": "This has been a brief summary of the problems emerging as more community hospitals develop neonatal intensive care, analyzing their possible origins and significance. A description of one solution to the problem has been given, exemplified by an outreach program from the University of California, San Diego, and University Hospital, San Diego, with the community of Las Vegas, Nevada. We feel strongly that the responsibilities of medical schools toward community hospitals and toward regionalization in isolated communities should be unique and are not covered by usual regulations or traditional concepts of regionalization.", "contents": "Neonatal intensive care in community hospitals and remote areas. The problems and a possible solution. This has been a brief summary of the problems emerging as more community hospitals develop neonatal intensive care, analyzing their possible origins and significance. A description of one solution to the problem has been given, exemplified by an outreach program from the University of California, San Diego, and University Hospital, San Diego, with the community of Las Vegas, Nevada. We feel strongly that the responsibilities of medical schools toward community hospitals and toward regionalization in isolated communities should be unique and are not covered by usual regulations or traditional concepts of regionalization.", "PMID": 963923}
{"id": "pubmed23n0032_5734", "title": "The large private hospital and neonatal intensive care.", "content": "The experiences of the Methodist Hospital of Indiana in integrating family physicians into neonatal intensive care are related, and some useful organizational innovations in neonatal nursing are suggested.", "contents": "The large private hospital and neonatal intensive care. The experiences of the Methodist Hospital of Indiana in integrating family physicians into neonatal intensive care are related, and some useful organizational innovations in neonatal nursing are suggested.", "PMID": 963924}
{"id": "pubmed23n0032_5735", "title": "Regionalization and service medicine.", "content": "A comprehensive plan for perinatal regionalization in the medical facilities of the military services is advocated. Factors peculiar to military medicine which must be recognized in planning are discussed.", "contents": "Regionalization and service medicine. A comprehensive plan for perinatal regionalization in the medical facilities of the military services is advocated. Factors peculiar to military medicine which must be recognized in planning are discussed.", "PMID": 963925}
{"id": "pubmed23n0032_5736", "title": "Principles of organization of an obstetrical unit from scratch.", "content": "The previous discussion has attempted to outline some basic principles in developing a high-risk obstetrical unit from scratch. Needless to say, emphasis in specific areas will be dictated by community needs, financial resources, numbers and training of personnel, and space limitations. The basic functions of patient care, education, and self-evaluation can be accomplished in any high-risk obstetrical unit.", "contents": "Principles of organization of an obstetrical unit from scratch. The previous discussion has attempted to outline some basic principles in developing a high-risk obstetrical unit from scratch. Needless to say, emphasis in specific areas will be dictated by community needs, financial resources, numbers and training of personnel, and space limitations. The basic functions of patient care, education, and self-evaluation can be accomplished in any high-risk obstetrical unit.", "PMID": 963926}
{"id": "pubmed23n0032_5737", "title": "Principles of organization of a neonatal intensive care unit from scratch.", "content": "This discussion has dealt with major principles to be considered when planning and organizing an infant special care unit. However, there remains one very important principle in the organization of any such unit: the job is never complete. As concepts and practices relative to care of the high-risk neonate change, so must organization of any unit delivering this care be modified and reorganized.", "contents": "Principles of organization of a neonatal intensive care unit from scratch. This discussion has dealt with major principles to be considered when planning and organizing an infant special care unit. However, there remains one very important principle in the organization of any such unit: the job is never complete. As concepts and practices relative to care of the high-risk neonate change, so must organization of any unit delivering this care be modified and reorganized.", "PMID": 963927}
{"id": "pubmed23n0032_5738", "title": "Preventing obsolescence in the design of a perinatal unit.", "content": "Basic principles are offered to support the suggestion that use of special care space must be made with a mind toward prevention of obsolescence. Certain conventional design features should be examined critically and decisions made as to their applicability to the needs of a particular program.", "contents": "Preventing obsolescence in the design of a perinatal unit. Basic principles are offered to support the suggestion that use of special care space must be made with a mind toward prevention of obsolescence. Certain conventional design features should be examined critically and decisions made as to their applicability to the needs of a particular program.", "PMID": 963929}
{"id": "pubmed23n0032_5739", "title": "Obstetrics: basic equipment needs.", "content": "The enunciation of equipment requirements has been attempted as needs appear today. Detailed and technical specifications for some of this equipment must be understood prior to major acquisitions. An awareness of current trends in clinical research can often be obtained from appropriate consultants which should forestall obsolescence. Finally, it must be reemphasized that the proper and intelligent use of equipment or diagnostic methods is essential. Likewise, the need to employ many of these techniques may become apparent only by paying close attention to the patient's clinical historical comments or as a result of skilled clinical observations.", "contents": "Obstetrics: basic equipment needs. The enunciation of equipment requirements has been attempted as needs appear today. Detailed and technical specifications for some of this equipment must be understood prior to major acquisitions. An awareness of current trends in clinical research can often be obtained from appropriate consultants which should forestall obsolescence. Finally, it must be reemphasized that the proper and intelligent use of equipment or diagnostic methods is essential. Likewise, the need to employ many of these techniques may become apparent only by paying close attention to the patient's clinical historical comments or as a result of skilled clinical observations.", "PMID": 963930}
{"id": "pubmed23n0032_5740", "title": "Equipment maintenance.", "content": "Basic to the success of the perinatal intensive care unit is a cooperative liasion between the medical and nursing staff and the clinical engineering staff. To this end the functions of the clinical engineering staff are preventive maintenance, inventory documentation, pre-purchase evaluation, emergency repair, personnel education, and management integration.", "contents": "Equipment maintenance. Basic to the success of the perinatal intensive care unit is a cooperative liasion between the medical and nursing staff and the clinical engineering staff. To this end the functions of the clinical engineering staff are preventive maintenance, inventory documentation, pre-purchase evaluation, emergency repair, personnel education, and management integration.", "PMID": 963932}
{"id": "pubmed23n0032_5741", "title": "Laboratory and blood bank needs of a perinatal center.", "content": "Prenatal and neonatal laboratory diagnostic tests are discussed in terms of purpose, technique, and evaluation of results. The need for quick access to fresh blood products must be appreciated by the blood bank, and the perinatologist must have a thorough understanding of blood products and the need for established procedures to guarantee safe transfusion.", "contents": "Laboratory and blood bank needs of a perinatal center. Prenatal and neonatal laboratory diagnostic tests are discussed in terms of purpose, technique, and evaluation of results. The need for quick access to fresh blood products must be appreciated by the blood bank, and the perinatologist must have a thorough understanding of blood products and the need for established procedures to guarantee safe transfusion.", "PMID": 963933}
{"id": "pubmed23n0032_5742", "title": "Diagnostic radiography and ultrasound in the perinatal intensive care unit.", "content": "A range of possible solutions to common problems associated with radiographic examination of the newborn is presented. The various modes in which ultrasound is currently used as a diagnostic modality are reviewed together with recent innovations that have greatly increased the clinical utility of the technique.", "contents": "Diagnostic radiography and ultrasound in the perinatal intensive care unit. A range of possible solutions to common problems associated with radiographic examination of the newborn is presented. The various modes in which ultrasound is currently used as a diagnostic modality are reviewed together with recent innovations that have greatly increased the clinical utility of the technique.", "PMID": 963934}
{"id": "pubmed23n0032_5743", "title": "Climate and environmental concepts.", "content": "What constitutes an optimal environment is still under question, but active research involves determining which external stimuli are beneficial and which are detrimental to infants. Temperature control and visual, auditory, proprioceptive, and tactile stimulation are discussed.", "contents": "Climate and environmental concepts. What constitutes an optimal environment is still under question, but active research involves determining which external stimuli are beneficial and which are detrimental to infants. Temperature control and visual, auditory, proprioceptive, and tactile stimulation are discussed.", "PMID": 963935}
{"id": "pubmed23n0032_5744", "title": "Family centered maternity care: its relationship to perinatal regionalization and neonatal intensive care.", "content": "For several months prior to birth a major portion of a family's attention, conversation, thought, and often worry, is directed toward the idea of a new child. This prolonged attention and anticipation contribute to making childbirth an emotionally charged experience. In psychological terms, it is therefore a critical period of peak motivation for learning, and a time to peak susceptibility to reinforcement. Theory, reason, and scientific evidence indicate thng with childbirth and early postpartum experiences, can significantly affect subsequent parental behaviors, the child's central environment influence. Evidence strongly suggests that these parental attitudes and behaviors so crucial to the child's ultimate well-being are learned rather than derived instinctually, and therefore they are malleable and can be taught, directed, and corrected. Through education and reinforcement it is possible to encourage parental behaviors and child interactions which are products of feelings of control, competence, accomplishment, understanding, and caring. Similarly we can recognize and work toward replacing attitudes, feelings, and behaviors that express fear, worry, and insecurity about the child. Over the past 50 years major changes have occurred in the practice of obstetrics and newborn pediatrics. Other major changes will necessarily occur as we move toward perinatal regionalization. Changes instigated solely on physiologic data can have unrecognized collateral effects on the psychological component of the childbirth experience. All concerned health care personnel, especially obstetricians and pediatricians, can insist that the importance of desirable mother-father-child interactions be recognized and that practices fostering them be afforded a high priority. I would like to endorse a comment from a recent article by Richmond concerning the advent of behavioral pediatrics by adding that behavioral obstetrics is also \"an idea whose time has arrived\".", "contents": "Family centered maternity care: its relationship to perinatal regionalization and neonatal intensive care. For several months prior to birth a major portion of a family's attention, conversation, thought, and often worry, is directed toward the idea of a new child. This prolonged attention and anticipation contribute to making childbirth an emotionally charged experience. In psychological terms, it is therefore a critical period of peak motivation for learning, and a time to peak susceptibility to reinforcement. Theory, reason, and scientific evidence indicate thng with childbirth and early postpartum experiences, can significantly affect subsequent parental behaviors, the child's central environment influence. Evidence strongly suggests that these parental attitudes and behaviors so crucial to the child's ultimate well-being are learned rather than derived instinctually, and therefore they are malleable and can be taught, directed, and corrected. Through education and reinforcement it is possible to encourage parental behaviors and child interactions which are products of feelings of control, competence, accomplishment, understanding, and caring. Similarly we can recognize and work toward replacing attitudes, feelings, and behaviors that express fear, worry, and insecurity about the child. Over the past 50 years major changes have occurred in the practice of obstetrics and newborn pediatrics. Other major changes will necessarily occur as we move toward perinatal regionalization. Changes instigated solely on physiologic data can have unrecognized collateral effects on the psychological component of the childbirth experience. All concerned health care personnel, especially obstetricians and pediatricians, can insist that the importance of desirable mother-father-child interactions be recognized and that practices fostering them be afforded a high priority. I would like to endorse a comment from a recent article by Richmond concerning the advent of behavioral pediatrics by adding that behavioral obstetrics is also \"an idea whose time has arrived\".", "PMID": 963936}
{"id": "pubmed23n0032_5745", "title": "Family crisis care.", "content": "By examining aspects of the family crisis care program in the Newborn Special Care Unit at Yale-New Haven Hospital, an illustration is made of one approach to utilizing the theory of crisis intervention in the development of a family oriented program of preventive intervention. The elements reviewed are adaptable as a framework for initiating such a program or as a reference for assessing programs now under development in other perinatal centers.", "contents": "Family crisis care. By examining aspects of the family crisis care program in the Newborn Special Care Unit at Yale-New Haven Hospital, an illustration is made of one approach to utilizing the theory of crisis intervention in the development of a family oriented program of preventive intervention. The elements reviewed are adaptable as a framework for initiating such a program or as a reference for assessing programs now under development in other perinatal centers.", "PMID": 963938}
{"id": "pubmed23n0032_5746", "title": "Training of personnel-pediatrics.", "content": "The educational programs that have been developed in newborn special care facilities have two purposes: first, they teach personnel the manual skills that are necessary to manage the sick newborn infant; second, they provide personnel with a better understanding of neonatal pathophysiology so that they can begin to recognize the subtle responses of the sick neonate. With these skills and knowledge, the nursery staff assumes a more active role in the team's effort to preserve a child's life.", "contents": "Training of personnel-pediatrics. The educational programs that have been developed in newborn special care facilities have two purposes: first, they teach personnel the manual skills that are necessary to manage the sick newborn infant; second, they provide personnel with a better understanding of neonatal pathophysiology so that they can begin to recognize the subtle responses of the sick neonate. With these skills and knowledge, the nursery staff assumes a more active role in the team's effort to preserve a child's life.", "PMID": 963940}
{"id": "pubmed23n0032_5747", "title": "The infant transport service.", "content": "The ultimate goal of an infant transport service is the reduction of neonatal morbidity and mortality in a given area. In order to attain this goal, the program must provide for the controlled transfer of a stabilized infant. Sub-objectives will include but not be limited to the integration of a readily available and knowledgeable ambulance service, the selection of appropriate transport teams, the establishment and maintenance of ongoing communications between all parties, the teaching of neonatal medicine to stimulate thereferring hospital to begin infant stabilization, and the constant evaluation of the overall performance of the infant transport system to detect defects in the system.", "contents": "The infant transport service. The ultimate goal of an infant transport service is the reduction of neonatal morbidity and mortality in a given area. In order to attain this goal, the program must provide for the controlled transfer of a stabilized infant. Sub-objectives will include but not be limited to the integration of a readily available and knowledgeable ambulance service, the selection of appropriate transport teams, the establishment and maintenance of ongoing communications between all parties, the teaching of neonatal medicine to stimulate thereferring hospital to begin infant stabilization, and the constant evaluation of the overall performance of the infant transport system to detect defects in the system.", "PMID": 963942}
{"id": "pubmed23n0032_5748", "title": "Intraoral cancer of the minor salivary glands.", "content": "Intraoral minor salivary gland carcinomas are relatively uncommon. The glands are concentrated in the mucosa of the mouth but occur over a wide area in the head and neck region. Little is known about the etiologic factors responsible for these tumors. Histologically, most salivary gland tumors are similar to those occurring in the parotid gland, but are more likely to be malignant. The most common presentations is an asymptomatic submucosal mass on the palate in a patient in the fifth or sixth decade, although it can occur in any age group. Neck node metastases occur in about one fourth of these patients. Surgical resection is the treatment of choice; irradiation may be a useful adjunctive method of palliation. Multiple recurrences over a long period of time are the rule, with 5 year survival for all malignant lesions being 44.5 per cent. The survival decreases another 10 per cent for each succeeding 5-year period of follow-up.", "contents": "Intraoral cancer of the minor salivary glands. Intraoral minor salivary gland carcinomas are relatively uncommon. The glands are concentrated in the mucosa of the mouth but occur over a wide area in the head and neck region. Little is known about the etiologic factors responsible for these tumors. Histologically, most salivary gland tumors are similar to those occurring in the parotid gland, but are more likely to be malignant. The most common presentations is an asymptomatic submucosal mass on the palate in a patient in the fifth or sixth decade, although it can occur in any age group. Neck node metastases occur in about one fourth of these patients. Surgical resection is the treatment of choice; irradiation may be a useful adjunctive method of palliation. Multiple recurrences over a long period of time are the rule, with 5 year survival for all malignant lesions being 44.5 per cent. The survival decreases another 10 per cent for each succeeding 5-year period of follow-up.", "PMID": 963951}
{"id": "pubmed23n0032_5749", "title": "Function and appearance following surgery for intraoral cancer.", "content": "Operations for intraoral carcinoma do produce deformities and loss of function. Historically, surgeons have constantly tried to correct or improve these deficiencies. The tendency on the part of the surgeon is to replace each bit of tissue removed, hoping that if the site appears normal the patient will be normal. The appearance of the patient has as a rule been given more attention than the functional result. Dysfunction is related to the alteration of certain anatomic and physiologic conditions identified as: (1) inability to obliterate the anterior oral cavity, (2) defects in the palate, (3) inability to close off the oropharyngeal space, and (4) disturbance of normal channels to bypass the larynx. Lateral mandibulectomy and radical neck dissection performed for cancer of the retromolar area, lower gum, lateral tongue, or floor of the mouth will in most instances result in very acceptable cosmetic and functional results. These patients look well, speak well, eat and swallow well, and can assume their usual role in society. Further surgical attempts to improve any deficiencies are discouraged. Patients sometimes ask about bone grafting or devices to replace the resected mandible and our recommendations are very emphatic on the negative side.", "contents": "Function and appearance following surgery for intraoral cancer. Operations for intraoral carcinoma do produce deformities and loss of function. Historically, surgeons have constantly tried to correct or improve these deficiencies. The tendency on the part of the surgeon is to replace each bit of tissue removed, hoping that if the site appears normal the patient will be normal. The appearance of the patient has as a rule been given more attention than the functional result. Dysfunction is related to the alteration of certain anatomic and physiologic conditions identified as: (1) inability to obliterate the anterior oral cavity, (2) defects in the palate, (3) inability to close off the oropharyngeal space, and (4) disturbance of normal channels to bypass the larynx. Lateral mandibulectomy and radical neck dissection performed for cancer of the retromolar area, lower gum, lateral tongue, or floor of the mouth will in most instances result in very acceptable cosmetic and functional results. These patients look well, speak well, eat and swallow well, and can assume their usual role in society. Further surgical attempts to improve any deficiencies are discouraged. Patients sometimes ask about bone grafting or devices to replace the resected mandible and our recommendations are very emphatic on the negative side.", "PMID": 963952}
{"id": "pubmed23n0032_5750", "title": "Intraoral reconstruction in head and neck cancer surgery.", "content": "Numerous techniques are available to resurface the oral cavity. These range from the very simple and straightforward to the very difficult and complex. Certainly for small lesions, local excision and direct approximation of mucosa is all that is necessary. For larger defects, particularly those which will require some other form of reconstruction, additional tissue must be brought into the operative defect. For superficial excisions, skin or mucosal grafts are adequate to resurface the mouth. For areas of extensive resection, flaps of some type are required. The use of local random flaps, regional arterialized flaps, and myocutaneous flaps have been described. The decision for their use must be as individual as each patient. Free flap transfer has specific theoretical benefits. The advantages and disadvantages of each technique have been presented and, it is hoped, will allow the reconstructive surgeon greater latitude in selecting a technique of reconstruction.", "contents": "Intraoral reconstruction in head and neck cancer surgery. Numerous techniques are available to resurface the oral cavity. These range from the very simple and straightforward to the very difficult and complex. Certainly for small lesions, local excision and direct approximation of mucosa is all that is necessary. For larger defects, particularly those which will require some other form of reconstruction, additional tissue must be brought into the operative defect. For superficial excisions, skin or mucosal grafts are adequate to resurface the mouth. For areas of extensive resection, flaps of some type are required. The use of local random flaps, regional arterialized flaps, and myocutaneous flaps have been described. The decision for their use must be as individual as each patient. Free flap transfer has specific theoretical benefits. The advantages and disadvantages of each technique have been presented and, it is hoped, will allow the reconstructive surgeon greater latitude in selecting a technique of reconstruction.", "PMID": 963953}
{"id": "pubmed23n0032_5751", "title": "Haemodynamics and body fluid volumes in response to fluid loading in conscious dogs: non-excretory renal influences.", "content": "1. Twelve conscious, chronically instrumented dogs were subjected to rapid loading with sodium chloride solution (150 mmol/1; saline) before and 1 day after bilateral nephrectomy (six dogs) or ureterocaval anastomosis (six dogs). Measurements were performed up to 3 h after the fluid load and included cardiac output with an electromagnetic flowmeter, mean arterial pressure and right atrial pressure with chronically implanted catheters, interstitial fluid pressure with a plastic capsule, heart rate, extracellular fluid volume, erythrocyte volume, plasma volume, plasma protein concentration and other variables. 2. The increase in cardiac output in response to saline load was significantly prolonged in the anephric dogs compared with those with uretero-caval anastomosis; mean arterial pressure, right atrial pressure and heart-rate changes were similar in both groups. 3. Plasma volume appeared to increase more in the anephric dogs than in those with uretero-caval anastomosis during the first hour after the infusion, although conflicting results were obtained with different estimates of plasma volume changes. Interstitial fluid pressure increased significantly less in the anephric dogs in the early stages of the fluid load. 4. Effective vascular compliance (the ratio of the change in blood volume to the change in right atrial pressure) appeared increased in the anephric dogs. On the other hand, the change in cardiac output for a given change in right atrial pressure was found to increase after bilateral nephrectomy. 5. It is suggested that the prolonged increase in cardiac output observed in anephric dogs was not the consequence of preferential plasma volume expansion nor of decreased venous compliance, but may reflect an alteration in the cardiac function curve.", "contents": "Haemodynamics and body fluid volumes in response to fluid loading in conscious dogs: non-excretory renal influences. 1. Twelve conscious, chronically instrumented dogs were subjected to rapid loading with sodium chloride solution (150 mmol/1; saline) before and 1 day after bilateral nephrectomy (six dogs) or ureterocaval anastomosis (six dogs). Measurements were performed up to 3 h after the fluid load and included cardiac output with an electromagnetic flowmeter, mean arterial pressure and right atrial pressure with chronically implanted catheters, interstitial fluid pressure with a plastic capsule, heart rate, extracellular fluid volume, erythrocyte volume, plasma volume, plasma protein concentration and other variables. 2. The increase in cardiac output in response to saline load was significantly prolonged in the anephric dogs compared with those with uretero-caval anastomosis; mean arterial pressure, right atrial pressure and heart-rate changes were similar in both groups. 3. Plasma volume appeared to increase more in the anephric dogs than in those with uretero-caval anastomosis during the first hour after the infusion, although conflicting results were obtained with different estimates of plasma volume changes. Interstitial fluid pressure increased significantly less in the anephric dogs in the early stages of the fluid load. 4. Effective vascular compliance (the ratio of the change in blood volume to the change in right atrial pressure) appeared increased in the anephric dogs. On the other hand, the change in cardiac output for a given change in right atrial pressure was found to increase after bilateral nephrectomy. 5. It is suggested that the prolonged increase in cardiac output observed in anephric dogs was not the consequence of preferential plasma volume expansion nor of decreased venous compliance, but may reflect an alteration in the cardiac function curve.", "PMID": 963954}
{"id": "pubmed23n0032_5752", "title": "The esterification of exogenous fatty acids by adipose tissue of hypertriglyceridaemic subjects with or without diabetes mellitus.", "content": "1. The esterification of exogenous palmitate to diglyceride and triglyceride in adipocytes was studied in obese and diabetic patients with and without hypertriglyceridaemia. The rate of esterification correlated significantly with the triglyceride content of adipocytes. 2. In diabetic patients with hypertriglyceridaemia, the rate of esterification to triglyceride was significantly greater than in diabetic patients with normotriglyceridaemia. This difference could not be attributed to differences in glucose tolerance or to the degree of obesity. 3. Fasting plasma insulin levels were greater in the hypertriglyceridaemic group than in the normotriglyceridaemic group. The difference in esterification rates could have been due to differences in adipocyte size. 4. The esterification of fatty acid in adipose tissue of diabetic patients was lower than in non-diabetic subjects and this difference could not be accounted for by differences in adipocyte size or differences in the intracellular pools of fatty acid in adipose tissue. 5. The role of esterification of exogenous fatty acids in adipose tissue as a possible determinant of the uptake of glyceride fatty acids from plasma is discussed.", "contents": "The esterification of exogenous fatty acids by adipose tissue of hypertriglyceridaemic subjects with or without diabetes mellitus. 1. The esterification of exogenous palmitate to diglyceride and triglyceride in adipocytes was studied in obese and diabetic patients with and without hypertriglyceridaemia. The rate of esterification correlated significantly with the triglyceride content of adipocytes. 2. In diabetic patients with hypertriglyceridaemia, the rate of esterification to triglyceride was significantly greater than in diabetic patients with normotriglyceridaemia. This difference could not be attributed to differences in glucose tolerance or to the degree of obesity. 3. Fasting plasma insulin levels were greater in the hypertriglyceridaemic group than in the normotriglyceridaemic group. The difference in esterification rates could have been due to differences in adipocyte size. 4. The esterification of fatty acid in adipose tissue of diabetic patients was lower than in non-diabetic subjects and this difference could not be accounted for by differences in adipocyte size or differences in the intracellular pools of fatty acid in adipose tissue. 5. The role of esterification of exogenous fatty acids in adipose tissue as a possible determinant of the uptake of glyceride fatty acids from plasma is discussed.", "PMID": 963955}
{"id": "pubmed23n0032_5753", "title": "The response of arginine vasopressin and plasma renin to postural change in normal man, with observations on syncope.", "content": "1. Fourteen mildly hydropenic normal volunteers were slowly tilted at a constant rate from the horizontal to the 85 degrees head-up position in order to study the interrelationship between plasma arginine vasopressin concentration, plasma renin activity and the change of plasma volume. 2. Nine subjects did not develop vaso-vagal symptoms and were studied for 45-60 min. Arginine vasopressin rose biphasically in all subjects: a small initial rise, which was seen at 3 min and persisted for 30 min, was followed by a striking rise between 30 and 45 min, when the fall of plasma volume had reached its maximum (17%). 3. Plasma renin activity reached a maximum at 30 min but fell by 45 min, as plasma concentration of arginine vasopressin rose. 4. Five subjects developed vaso-vagal symptoms 4-24 min after reaching 85 degrees when the study was terminated. A striking increase of arginine vasopressin concentration was seen within 4 min of syncope, but there was no change of plasma osmolality, cortisol concentration or renin activity.", "contents": "The response of arginine vasopressin and plasma renin to postural change in normal man, with observations on syncope. 1. Fourteen mildly hydropenic normal volunteers were slowly tilted at a constant rate from the horizontal to the 85 degrees head-up position in order to study the interrelationship between plasma arginine vasopressin concentration, plasma renin activity and the change of plasma volume. 2. Nine subjects did not develop vaso-vagal symptoms and were studied for 45-60 min. Arginine vasopressin rose biphasically in all subjects: a small initial rise, which was seen at 3 min and persisted for 30 min, was followed by a striking rise between 30 and 45 min, when the fall of plasma volume had reached its maximum (17%). 3. Plasma renin activity reached a maximum at 30 min but fell by 45 min, as plasma concentration of arginine vasopressin rose. 4. Five subjects developed vaso-vagal symptoms 4-24 min after reaching 85 degrees when the study was terminated. A striking increase of arginine vasopressin concentration was seen within 4 min of syncope, but there was no change of plasma osmolality, cortisol concentration or renin activity.", "PMID": 963956}
{"id": "pubmed23n0032_5754", "title": "Influence of sodium restriction upon two models of renal hypertension.", "content": "1. Restriction of dietary salt intake does not affect the development or maintenance of hypertension in rats with unilateral renal ischaemia whether the contralateral kidney is present (Goldblatt 2 model) or not (Goldblatt 1 model). 2. Acute dietary salt depletion induces a similar loss of sodium and fall in body weight with little change in blood pressure in both normal and hypertensive rats. 3. Excision of the ischaemic kidney in rats with short-term (less than 50 days) Goldblatt 2 hypertension restores the mean blood pressure to normal, whereas Goldblatt 1 hypertensive rats show only a partial response. Previous salt depletion of this model enhances the blood pressure response to nephrectomy. 4. Sodium retention plays no part in the development or maintenance of Goldblatt 2 kidney hypertension. However, although sodium retention is normally involved in the Goldblatt 1 model, hypertension can develop in the absence of dietary sodium.", "contents": "Influence of sodium restriction upon two models of renal hypertension. 1. Restriction of dietary salt intake does not affect the development or maintenance of hypertension in rats with unilateral renal ischaemia whether the contralateral kidney is present (Goldblatt 2 model) or not (Goldblatt 1 model). 2. Acute dietary salt depletion induces a similar loss of sodium and fall in body weight with little change in blood pressure in both normal and hypertensive rats. 3. Excision of the ischaemic kidney in rats with short-term (less than 50 days) Goldblatt 2 hypertension restores the mean blood pressure to normal, whereas Goldblatt 1 hypertensive rats show only a partial response. Previous salt depletion of this model enhances the blood pressure response to nephrectomy. 4. Sodium retention plays no part in the development or maintenance of Goldblatt 2 kidney hypertension. However, although sodium retention is normally involved in the Goldblatt 1 model, hypertension can develop in the absence of dietary sodium.", "PMID": 963957}
{"id": "pubmed23n0032_5755", "title": "Potassium transport across the distal colon in man.", "content": "1. The mechanism of potassium transport across human distal colon was investigated in twenty-two individuals without evidence of bowel disease, by using a dialysis method in conjunction with measurements of the transepithelial potential difference (p.d.). 2. Whether potassium was absorbed or secreted depended on its initial concentration in the lumen. The potassium net flux was approximately zero when the luminal potassium concentration was between 30 and 50 mmol/l. 3. Potassium secretion rate was little affected by sodium absorption rate, or by the luminal sodium concentration or by the osmolality of the luminal solution. 4. Potassium secretion rate was increased by partial substitution of other cations for sodium, in the descending order Li greater than NH4 greater than Rb greater than Na. Potassium concentration increased on average to over 80 mmol/l when lithium was in the lumen. 5. The observed transepithelial p.d. was inadequate to account for the intraluminal potassium concentrations attained, the discrepancy being most marked when ammonium or lithium was in the lumen.  It is suggested that some potassium is secreted by the epithelial cells and this component of the total potassium flux into the lumen is increased when rubidium, ammonium or lithium is substituted for sodium.", "contents": "Potassium transport across the distal colon in man. 1. The mechanism of potassium transport across human distal colon was investigated in twenty-two individuals without evidence of bowel disease, by using a dialysis method in conjunction with measurements of the transepithelial potential difference (p.d.). 2. Whether potassium was absorbed or secreted depended on its initial concentration in the lumen. The potassium net flux was approximately zero when the luminal potassium concentration was between 30 and 50 mmol/l. 3. Potassium secretion rate was little affected by sodium absorption rate, or by the luminal sodium concentration or by the osmolality of the luminal solution. 4. Potassium secretion rate was increased by partial substitution of other cations for sodium, in the descending order Li greater than NH4 greater than Rb greater than Na. Potassium concentration increased on average to over 80 mmol/l when lithium was in the lumen. 5. The observed transepithelial p.d. was inadequate to account for the intraluminal potassium concentrations attained, the discrepancy being most marked when ammonium or lithium was in the lumen.  It is suggested that some potassium is secreted by the epithelial cells and this component of the total potassium flux into the lumen is increased when rubidium, ammonium or lithium is substituted for sodium.", "PMID": 963958}
{"id": "pubmed23n0032_5756", "title": "Blood flow through the calf during recovery from fractures of the lower limb.", "content": "1. Venous occlusion plethysmography has been used to measure the blood flow in the calves of nineteen patients with fractures of the lower limb and in six normal control subjects. 2. The resting blood flows were significantly higher in the injured legs than in uninjured legs, irrespective of the site of injury. Flows in the uninjured limbs were similar to those of the control subjects. 3. During reactive hyperaemia after 10 min arterial occlusion, the increase of flows in both legs of the patients was significantly lower than in the control subjects. Because of the increased resting flow, the maximal flow in the injured leg was similar to that in the control subjects, whereas the maximal flow in the uninjured leg was significantly lower than in the control group. 4. The changes in resting flow cannot be accounted for by a change in the proportions of tissues in the limb but they may be explained by an increase of the flow through muscle secondary to a relative increase in the mass of slow to fast muscle fibres.", "contents": "Blood flow through the calf during recovery from fractures of the lower limb. 1. Venous occlusion plethysmography has been used to measure the blood flow in the calves of nineteen patients with fractures of the lower limb and in six normal control subjects. 2. The resting blood flows were significantly higher in the injured legs than in uninjured legs, irrespective of the site of injury. Flows in the uninjured limbs were similar to those of the control subjects. 3. During reactive hyperaemia after 10 min arterial occlusion, the increase of flows in both legs of the patients was significantly lower than in the control subjects. Because of the increased resting flow, the maximal flow in the injured leg was similar to that in the control subjects, whereas the maximal flow in the uninjured leg was significantly lower than in the control group. 4. The changes in resting flow cannot be accounted for by a change in the proportions of tissues in the limb but they may be explained by an increase of the flow through muscle secondary to a relative increase in the mass of slow to fast muscle fibres.", "PMID": 963959}
{"id": "pubmed23n0032_5757", "title": "Effects of immersion in water and changes in intrathoracic blood volume on lung function in man.", "content": "1. In healthy, normal subjects simultaneous peripheral venous occlusion of all four limbs caused a small but significant increase in vital capacity (VC) and single-breath carbon monoxide transfer factor (DLCO) without significantly changing total lung capacity (TLC), residual volume (RV), pulmonary gas flow or pulmonary compliance. 2. Immersion in water to the neck resulted in a small but significant fall in VC, FEV 1.0/FVC and TLC, and a rise in DLCO, but flow/volume curves and 'closing volume' were unchanged. Peripheral venous occlusion during immersion only significantly increased VC and DLCO; pulmonary compliance and flow/volume curves did not alter significantly. 3. It is concluded that peripheral venous occlusion produces these effects by altering intrathoracic blood volume. Water immersion reduces TLC, mainly from the hydrostatic pressure, and VC is reduced from both the hydrostatic pressure and the increase in intrathoracic blood volume consequent on immersion. The increase in DLCO is due, almost entirely, to the increase in intrathoracic blood volume.", "contents": "Effects of immersion in water and changes in intrathoracic blood volume on lung function in man. 1. In healthy, normal subjects simultaneous peripheral venous occlusion of all four limbs caused a small but significant increase in vital capacity (VC) and single-breath carbon monoxide transfer factor (DLCO) without significantly changing total lung capacity (TLC), residual volume (RV), pulmonary gas flow or pulmonary compliance. 2. Immersion in water to the neck resulted in a small but significant fall in VC, FEV 1.0/FVC and TLC, and a rise in DLCO, but flow/volume curves and 'closing volume' were unchanged. Peripheral venous occlusion during immersion only significantly increased VC and DLCO; pulmonary compliance and flow/volume curves did not alter significantly. 3. It is concluded that peripheral venous occlusion produces these effects by altering intrathoracic blood volume. Water immersion reduces TLC, mainly from the hydrostatic pressure, and VC is reduced from both the hydrostatic pressure and the increase in intrathoracic blood volume consequent on immersion. The increase in DLCO is due, almost entirely, to the increase in intrathoracic blood volume.", "PMID": 963960}
{"id": "pubmed23n0032_5758", "title": "Common bacterial urinary tract infections in women.", "content": "Unfortunately, there is no general consensus as to how long patients with bacteriuria or urinary tract infections should be monitored and certainly there is no agreement on how long recurrent episodes should be treated beyond ten days to two weeks. The most important points to remember are: 1. Culture the urine both at the time of therapy and during follow-up. The patient should be examined periodically for the presence of bacteruria. If bacteria cannot be eradicated, at least the physician is aware of the organism most likely causing the patient's symptoms. 2. Do not subject the patient with frequent recurrent (chronic) and complicated infections to continual antibacterial therapy, but rather, manage the acute episodes. 3. Use prophylaxis, particularly single bed-time doses for dysuria and frequency symptoms. 4. Screen for bacteriuria during pregnancy. 5. Avoid the use of catheters except where absolutely necessary. 6. Avoid systemic prophylaxis of infection in patients with catheters; rather, use closed-system drainage with antibacteri-irrigation. It is to be hoped within the next few years, studies now underway will allow specific recommendations regarding the management of asymptomatic bacteruria, the duration of therapy for recurrent infections, the prevention and treatment of L-form bacterial infections, and indications for urologic procedures.", "contents": "Common bacterial urinary tract infections in women. Unfortunately, there is no general consensus as to how long patients with bacteriuria or urinary tract infections should be monitored and certainly there is no agreement on how long recurrent episodes should be treated beyond ten days to two weeks. The most important points to remember are: 1. Culture the urine both at the time of therapy and during follow-up. The patient should be examined periodically for the presence of bacteruria. If bacteria cannot be eradicated, at least the physician is aware of the organism most likely causing the patient's symptoms. 2. Do not subject the patient with frequent recurrent (chronic) and complicated infections to continual antibacterial therapy, but rather, manage the acute episodes. 3. Use prophylaxis, particularly single bed-time doses for dysuria and frequency symptoms. 4. Screen for bacteriuria during pregnancy. 5. Avoid the use of catheters except where absolutely necessary. 6. Avoid systemic prophylaxis of infection in patients with catheters; rather, use closed-system drainage with antibacteri-irrigation. It is to be hoped within the next few years, studies now underway will allow specific recommendations regarding the management of asymptomatic bacteruria, the duration of therapy for recurrent infections, the prevention and treatment of L-form bacterial infections, and indications for urologic procedures.", "PMID": 963966}
{"id": "pubmed23n0032_5759", "title": "Effects of prostaglandin E2 and F2alpha therapy.", "content": "Prostaglandins are widely  distributed in the nervous system and have important effects on nerve cells. Prostaglandins of the E series tend to cause sedation, whereas this action is much less obvious with F prostaglandins. Prostaglandin therapy for termination of pregnancy is usually administered by the intrauterine route, which means that only very low concentrations of prostaglandin E2 or prostaglandin F2alpha reach the cerebral circulation after passage through the highly effecient metabolizing system in the lungs. The blood-brain barrier may also reduce the amount of prostaglandin reaching the nerve cells.However, prostaglandin F2alpha appears to have been responsible for major convulsions in a small proportion of patients, and the appearance of epileptiform spike activity has been clearly demonstrated in some patients with normal pretreatment EEGs. Prostaglandin E2 has not been reported to cause convulsions or epileptiform spike activity in normal women, although deterioration of an already abnormal EEG has been seen in one patient. Although the risk of convulsions with prostaglandin F2alpha is low, it seems preferable to use prostalgandins E2 where possible, particularly on patients with a history of epilepsy.", "contents": "Effects of prostaglandin E2 and F2alpha therapy. Prostaglandins are widely  distributed in the nervous system and have important effects on nerve cells. Prostaglandins of the E series tend to cause sedation, whereas this action is much less obvious with F prostaglandins. Prostaglandin therapy for termination of pregnancy is usually administered by the intrauterine route, which means that only very low concentrations of prostaglandin E2 or prostaglandin F2alpha reach the cerebral circulation after passage through the highly effecient metabolizing system in the lungs. The blood-brain barrier may also reduce the amount of prostaglandin reaching the nerve cells.However, prostaglandin F2alpha appears to have been responsible for major convulsions in a small proportion of patients, and the appearance of epileptiform spike activity has been clearly demonstrated in some patients with normal pretreatment EEGs. Prostaglandin E2 has not been reported to cause convulsions or epileptiform spike activity in normal women, although deterioration of an already abnormal EEG has been seen in one patient. Although the risk of convulsions with prostaglandin F2alpha is low, it seems preferable to use prostalgandins E2 where possible, particularly on patients with a history of epilepsy.", "PMID": 963968}
{"id": "pubmed23n0032_5760", "title": "Emergency treatment of wounds of the colon.", "content": "Colonic wounds vary greatly in their severity and the number of concomitantly injured organs. Relatively few colonic wounds are single; these can usually be treated by primary repair. The approach to colonic wounds generally must be selective and take into account the degree of associated shock,contamination, and surrounding tissue damage. Judicious application of the wide variety of technical approaches available today has increased the incidence of primary repair to over 50% and has reduced mortality to 10% to 15%.", "contents": "Emergency treatment of wounds of the colon. Colonic wounds vary greatly in their severity and the number of concomitantly injured organs. Relatively few colonic wounds are single; these can usually be treated by primary repair. The approach to colonic wounds generally must be selective and take into account the degree of associated shock,contamination, and surrounding tissue damage. Judicious application of the wide variety of technical approaches available today has increased the incidence of primary repair to over 50% and has reduced mortality to 10% to 15%.", "PMID": 963969}
{"id": "pubmed23n0032_5761", "title": "[Psychiatry and humanitarianism].", "content": "Opposing positions on mental disorder in current psychiatry, their origins and therapy are presented through a few extreme viewpoints. Considering the foundations of the 'medical model' of psychiatric disorder (Griesinger), it is evident that this model is not a closed system, but rather an open approach which still has validity today. The humanitarian roots of psychiatry prevented Griesinger from treating various positions in an absolute or ideological manner. Finally, the concept of 'illness' is discussed in relation to psychophysiological activation research. Pathology changes in each different situation and is therefore not a static phenomenon. Mental disturbance is not determined by absolute measures, but rather by a reduction in variability of reactions to the particular situation. This concept of illness can determine somato-, psycho-, and sociotherapeutic measures. Psychiatry can only remain based on humanitarian concepts as long as the sciences upon which it is founded are kept in appropriate relation to one another.", "contents": "[Psychiatry and humanitarianism]. Opposing positions on mental disorder in current psychiatry, their origins and therapy are presented through a few extreme viewpoints. Considering the foundations of the 'medical model' of psychiatric disorder (Griesinger), it is evident that this model is not a closed system, but rather an open approach which still has validity today. The humanitarian roots of psychiatry prevented Griesinger from treating various positions in an absolute or ideological manner. Finally, the concept of 'illness' is discussed in relation to psychophysiological activation research. Pathology changes in each different situation and is therefore not a static phenomenon. Mental disturbance is not determined by absolute measures, but rather by a reduction in variability of reactions to the particular situation. This concept of illness can determine somato-, psycho-, and sociotherapeutic measures. Psychiatry can only remain based on humanitarian concepts as long as the sciences upon which it is founded are kept in appropriate relation to one another.", "PMID": 964006}
{"id": "pubmed23n0032_5762", "title": "Obesity: some psychological and physiological considerations.", "content": "The case report of an obese patient deals with his background, history of life and its correlation to obesity. The causes, circumstances and habits of eating are almost a perfect expression of the traditional theories of obesity. This case report emphasizes the correlation between the psychiatric theory that divides the obese people into adult and juvenile types and the histological findings that obesity can be divided into hyperplastic and hypertrophic types according to the quality and quantity of the fat cells. In fact these two theories may be manifestations of the basic, yet unknown cause of obesity. A doubt to the classical theories is cast by the operation of jejunoileostomy which changes many personality characteristics which were considered primary in obesity.", "contents": "Obesity: some psychological and physiological considerations. The case report of an obese patient deals with his background, history of life and its correlation to obesity. The causes, circumstances and habits of eating are almost a perfect expression of the traditional theories of obesity. This case report emphasizes the correlation between the psychiatric theory that divides the obese people into adult and juvenile types and the histological findings that obesity can be divided into hyperplastic and hypertrophic types according to the quality and quantity of the fat cells. In fact these two theories may be manifestations of the basic, yet unknown cause of obesity. A doubt to the classical theories is cast by the operation of jejunoileostomy which changes many personality characteristics which were considered primary in obesity.", "PMID": 964007}
{"id": "pubmed23n0032_5763", "title": "The dream of a twenty-four-year-old graduate student. Existential and Jungian considerations.", "content": "There are dreams that portray with unusual clarity critical junctures in a person's life. Inasmuch as the realm from which the images of a dream emerge is of the same nature as the source of genuine fairy tales, it is intuitively perceptive of the latter's deeper meanings. Thus suitable fairy tale themes can enhance the experiential insight into dreams. The young student's dream that we discussed indicates a sharp awareness of several existential disharmonies. He confuses the messages originating in his innermost self with external demands against which he still rebels blindly; he is incapable of metamorphosing creatively the numerous disorganized urges within himself; and he thus cannot achieve a positive relationship with his deeper potentials which are set aside. The comparison of the dream with an analogous, charming Romansh tale portraying a harmonious solution of the young man's impasse proved to be of crucial help.", "contents": "The dream of a twenty-four-year-old graduate student. Existential and Jungian considerations. There are dreams that portray with unusual clarity critical junctures in a person's life. Inasmuch as the realm from which the images of a dream emerge is of the same nature as the source of genuine fairy tales, it is intuitively perceptive of the latter's deeper meanings. Thus suitable fairy tale themes can enhance the experiential insight into dreams. The young student's dream that we discussed indicates a sharp awareness of several existential disharmonies. He confuses the messages originating in his innermost self with external demands against which he still rebels blindly; he is incapable of metamorphosing creatively the numerous disorganized urges within himself; and he thus cannot achieve a positive relationship with his deeper potentials which are set aside. The comparison of the dream with an analogous, charming Romansh tale portraying a harmonious solution of the young man's impasse proved to be of crucial help.", "PMID": 964008}
{"id": "pubmed23n0032_5764", "title": "Evaluation of the cytological examination in mass survey of gastric cancer.", "content": "The significance of the cytological examination in the diagnosis of gastric cancer, especially in the detection of the early cancer of the stomach, was examined in 1200 cases subjected to the cytological examination in the mass survey, including 181 cases with early cancer of the stomach, 148 cases with advanced cancer of the stomach. Comparison of the detection rates of cancer in cases of surgically confirmed stomach cancer revealed that cytological examination is more reliable than endoscopic and X-ray examinations of the stomach. In only a few instances, the false negative and false positive diagnoses were made, and therefore it is important for correct diagnoses to correlate the cytological finding with the other findings. The importance of cytological examination was clearly indicated by examination of the annual detection rate of early cancer, which showed a sudden increase after the period of the introduction of the cytological method in mass survey.", "contents": "Evaluation of the cytological examination in mass survey of gastric cancer. The significance of the cytological examination in the diagnosis of gastric cancer, especially in the detection of the early cancer of the stomach, was examined in 1200 cases subjected to the cytological examination in the mass survey, including 181 cases with early cancer of the stomach, 148 cases with advanced cancer of the stomach. Comparison of the detection rates of cancer in cases of surgically confirmed stomach cancer revealed that cytological examination is more reliable than endoscopic and X-ray examinations of the stomach. In only a few instances, the false negative and false positive diagnoses were made, and therefore it is important for correct diagnoses to correlate the cytological finding with the other findings. The importance of cytological examination was clearly indicated by examination of the annual detection rate of early cancer, which showed a sudden increase after the period of the introduction of the cytological method in mass survey.", "PMID": 964015}
{"id": "pubmed23n0032_5765", "title": "A new fiberintestinoscope type FIS-T1 patterned after intestinal biopsy tube.", "content": "A new type of fiberintestinoscope (FIS-T1) patterned after an intestinal biopsy tube was developed for observing the small intestine beyond the ligament of Treitz. The flexible part of the new FIS has been made as pliable as the Cantor tube or the Miller-Abbott tube so that the tip of the new FIS is carried on into the small intestine by peristaltic activity along its natural configuration. A balloon attached near the tip of the new FIS acted as a bolus, enabling peristaltic activity to carry the fiberscope to the distal portion. In order to facilitate the passage of the fiberscope, it was also advisable to keep changing the posture and position of the body with a minimum of fluoroscopic exposure. Inflation of the balloon as well as air feeding through the air channel of the fiberscope made possible satisfactory observation. In a patient suffering from protein-losing gastroenteropathy, multiple white spots on the diffuse rough surface of the mucosa were visualized by using the new FIS. Histological finding of the biopsy specimen taken from near the ligament of Treitz by using the fiberduodenoscope showed dilated lymph vessels in the villi.", "contents": "A new fiberintestinoscope type FIS-T1 patterned after intestinal biopsy tube. A new type of fiberintestinoscope (FIS-T1) patterned after an intestinal biopsy tube was developed for observing the small intestine beyond the ligament of Treitz. The flexible part of the new FIS has been made as pliable as the Cantor tube or the Miller-Abbott tube so that the tip of the new FIS is carried on into the small intestine by peristaltic activity along its natural configuration. A balloon attached near the tip of the new FIS acted as a bolus, enabling peristaltic activity to carry the fiberscope to the distal portion. In order to facilitate the passage of the fiberscope, it was also advisable to keep changing the posture and position of the body with a minimum of fluoroscopic exposure. Inflation of the balloon as well as air feeding through the air channel of the fiberscope made possible satisfactory observation. In a patient suffering from protein-losing gastroenteropathy, multiple white spots on the diffuse rough surface of the mucosa were visualized by using the new FIS. Histological finding of the biopsy specimen taken from near the ligament of Treitz by using the fiberduodenoscope showed dilated lymph vessels in the villi.", "PMID": 964016}
{"id": "pubmed23n0032_5766", "title": "Further studies on lipid-chemical differences between cancerous and adenomatous polypous tissues in the human large intestine.", "content": "17 cases of cancer and 18 cases of adenomatous polyp in the large intestine were subjected to lipid-chemical studies of their tissues in order to clarify the biochemical differences between malignant neoplastic growth and benign growth. Cancerous and adenomatous polypous tissues were collected by biopsy or surgical operation together with the respective control tissues. One part of the total lipid extracted from each tissue was separated into triglyceride and phospholipid fraction by a thin-layer chromatography (TLC). The fatty acid composition and content of each lipid fraction were measured by a gas-liquid chromatography (GLC). When the fatty acid composition of a phospholipid was expressed in terms of the deviation rate, that of C14:0 was remarkably increased and that of C20:4 was remarkably decreased in the cancerous cases as compared with the respective values of the cases of adenomatous polyp. More definite differences were recognized between the cancerous cases and the cases of adenomatous polyp, when the ratio of the deviation rate of C14:0 to that of C20:4 was calculated in each case. Namely, the ratios distributed between 2.064 and 4.125 in 10 cancerous cases, and between 0.393 and 1.856 in 16 cases of adenomatous polyp, indicating the biochemical differences between cancerous tissues and adenomatous polypous tissues.", "contents": "Further studies on lipid-chemical differences between cancerous and adenomatous polypous tissues in the human large intestine. 17 cases of cancer and 18 cases of adenomatous polyp in the large intestine were subjected to lipid-chemical studies of their tissues in order to clarify the biochemical differences between malignant neoplastic growth and benign growth. Cancerous and adenomatous polypous tissues were collected by biopsy or surgical operation together with the respective control tissues. One part of the total lipid extracted from each tissue was separated into triglyceride and phospholipid fraction by a thin-layer chromatography (TLC). The fatty acid composition and content of each lipid fraction were measured by a gas-liquid chromatography (GLC). When the fatty acid composition of a phospholipid was expressed in terms of the deviation rate, that of C14:0 was remarkably increased and that of C20:4 was remarkably decreased in the cancerous cases as compared with the respective values of the cases of adenomatous polyp. More definite differences were recognized between the cancerous cases and the cases of adenomatous polyp, when the ratio of the deviation rate of C14:0 to that of C20:4 was calculated in each case. Namely, the ratios distributed between 2.064 and 4.125 in 10 cancerous cases, and between 0.393 and 1.856 in 16 cases of adenomatous polyp, indicating the biochemical differences between cancerous tissues and adenomatous polypous tissues.", "PMID": 964017}
{"id": "pubmed23n0032_5767", "title": "Ischemic colitis.", "content": "Although several hundred cases of ischemic colitis have been reported from western countries, no case has been reported in our country. Ischemic colitis is a well established entity. Clinicopathological manifestations, disgnosis, treatment and prognosis of the disease are well known. A majority of reported cases were not particularly unusual. Two cases, of which one showed an unusually long lasting acute episode but good prognosis, and the other presented a diagnostic problem and was found to have a fibrous stricture due to ischemic colitis in the sigmoid colon at surgery, were described and discussed in relation to their symptoms, diagnosis and prognosis.", "contents": "Ischemic colitis. Although several hundred cases of ischemic colitis have been reported from western countries, no case has been reported in our country. Ischemic colitis is a well established entity. Clinicopathological manifestations, disgnosis, treatment and prognosis of the disease are well known. A majority of reported cases were not particularly unusual. Two cases, of which one showed an unusually long lasting acute episode but good prognosis, and the other presented a diagnostic problem and was found to have a fibrous stricture due to ischemic colitis in the sigmoid colon at surgery, were described and discussed in relation to their symptoms, diagnosis and prognosis.", "PMID": 964018}
{"id": "pubmed23n0032_5768", "title": "Chronic hepatitis: a follow-up study.", "content": "51 cases of chronic hepatitis were followed up by serial liver biopsies. 6 cases, all of which were of the active type, showed transition to liver cirrhosis within 5 to 10 months after the initial biopsy which had indicated chronic hepatitis. In 109 cases of liver cirrhosis, the antecedent acute hepatitis had been found within a relatively short period, usually 2 to 3 years before the establishment of the diagnosis of liver cirrhosis. Whether these cases which showed rapid transition to liver cirrhosis after acute hepatitis should be included in the group of chronic hepatitis or in another group remains to be settled.", "contents": "Chronic hepatitis: a follow-up study. 51 cases of chronic hepatitis were followed up by serial liver biopsies. 6 cases, all of which were of the active type, showed transition to liver cirrhosis within 5 to 10 months after the initial biopsy which had indicated chronic hepatitis. In 109 cases of liver cirrhosis, the antecedent acute hepatitis had been found within a relatively short period, usually 2 to 3 years before the establishment of the diagnosis of liver cirrhosis. Whether these cases which showed rapid transition to liver cirrhosis after acute hepatitis should be included in the group of chronic hepatitis or in another group remains to be settled.", "PMID": 964019}
{"id": "pubmed23n0032_5769", "title": "Clinical evaluation of iron metabolism in pancreatic disease.", "content": "In order to estimate the possible influence of pancreatic disease upon iron absorption, fecal analysis was carried out after administration of 5 mg iron labeled with 59Fe. Twelve cases of pancreatic calcification, 7 cases of chronic pancreatitis, 3 cases each of pancreatectomy and miscellaneous diseases, and 52 cases for control were studied. Significant elevation of iron absorption was observed in 4 or 21 patients with pancreatic diseases. Two of the 4 cases were also found to have low transferrin saturation and the other two have had a long history of heavy alcoholic intake. There was no definite correlation between the iron absorption rate and the total bicarbonate secretion, and duration of the diseases. Three cases treated with pancreatectomy failed to show increased iron absorption. The alternative administration of 59Fe and 55Fe for consecutive 4 days either alone or together with pancreatin or secretin was done to see the effect of these preparations on iron absorption. No significant inhibitory effect of these preparations on the iron absorption was noticed. These observations suggest that exocrine pancreatic function seldom appears to influence directly iron absorption, and that iron deficiency and ingestion of alcoholic beverages can be important factors affecting the iron absorption in pancreatic diseases.", "contents": "Clinical evaluation of iron metabolism in pancreatic disease. In order to estimate the possible influence of pancreatic disease upon iron absorption, fecal analysis was carried out after administration of 5 mg iron labeled with 59Fe. Twelve cases of pancreatic calcification, 7 cases of chronic pancreatitis, 3 cases each of pancreatectomy and miscellaneous diseases, and 52 cases for control were studied. Significant elevation of iron absorption was observed in 4 or 21 patients with pancreatic diseases. Two of the 4 cases were also found to have low transferrin saturation and the other two have had a long history of heavy alcoholic intake. There was no definite correlation between the iron absorption rate and the total bicarbonate secretion, and duration of the diseases. Three cases treated with pancreatectomy failed to show increased iron absorption. The alternative administration of 59Fe and 55Fe for consecutive 4 days either alone or together with pancreatin or secretin was done to see the effect of these preparations on iron absorption. No significant inhibitory effect of these preparations on the iron absorption was noticed. These observations suggest that exocrine pancreatic function seldom appears to influence directly iron absorption, and that iron deficiency and ingestion of alcoholic beverages can be important factors affecting the iron absorption in pancreatic diseases.", "PMID": 964020}
{"id": "pubmed23n0032_5770", "title": "Continuous secretin infusion with pancreozymin pretreatment as a pancreatic function test.", "content": "This paper deals with a pancreatic function test with the submaximal dose of secretin given by continuous infusion after injection of cholecystokinin-pancreozymin (CCK-PZ) in the submaximal dose. The active elimination of bladder bile by CCK-PZ pretreatment decreased the influence of biliary contamination, and the volume output and the bicarbonate concentration were stabilized by the continuous infusion of secretin. As a result, the coefficients of variation in the volume output and the bicarbonate concentration were calculated to be about 15 and about 5%, respectively. These variations were considerably smaller than those obtained in the standard secretin test. Therefore, the present study is expected to improve the diagnostic means for pancreatic excretory dysfunction.", "contents": "Continuous secretin infusion with pancreozymin pretreatment as a pancreatic function test. This paper deals with a pancreatic function test with the submaximal dose of secretin given by continuous infusion after injection of cholecystokinin-pancreozymin (CCK-PZ) in the submaximal dose. The active elimination of bladder bile by CCK-PZ pretreatment decreased the influence of biliary contamination, and the volume output and the bicarbonate concentration were stabilized by the continuous infusion of secretin. As a result, the coefficients of variation in the volume output and the bicarbonate concentration were calculated to be about 15 and about 5%, respectively. These variations were considerably smaller than those obtained in the standard secretin test. Therefore, the present study is expected to improve the diagnostic means for pancreatic excretory dysfunction.", "PMID": 964021}
{"id": "pubmed23n0032_5771", "title": "A diagnostic re-evaluation of electric skin resistance, skin temperature and deeper tenderness in patients with abdominal pain.", "content": "Diagnostic re-evaluation of measurement of electric skin resistance (ESR), skin temperature (ST) and deeper tenderness (DT) was performed in patients with abdominal pain due to pancreatitis, cholecystopathy and duodenal ulcer. These determinations were conducted when the pain was complained of and after the pain ceased by paravertebral anesthesia. ESR was decreased on the opposite tender points of the abdominal walls as compared with those values of the healthy abdominal walls. On the contrary, ESR was increased on the suffered body areas in patients with active myelitis. ESR was decreased on the abdominal walls where visceral pain was induced by inflation of a balloon attached to the apex of a Miller-Abbott double lumen tube. DT tended to show decrease, while ST a slight increase, when the pain was evoked. However, in these pain induced experiments, ST changes were not so remarkable as those of ESR. A viscero-cutaneous reflex machanism and the predominance of sympathetic nerve control might be possible causes to produce these changes. Several important factors influencing the determination of the ESR were also discussed.", "contents": "A diagnostic re-evaluation of electric skin resistance, skin temperature and deeper tenderness in patients with abdominal pain. Diagnostic re-evaluation of measurement of electric skin resistance (ESR), skin temperature (ST) and deeper tenderness (DT) was performed in patients with abdominal pain due to pancreatitis, cholecystopathy and duodenal ulcer. These determinations were conducted when the pain was complained of and after the pain ceased by paravertebral anesthesia. ESR was decreased on the opposite tender points of the abdominal walls as compared with those values of the healthy abdominal walls. On the contrary, ESR was increased on the suffered body areas in patients with active myelitis. ESR was decreased on the abdominal walls where visceral pain was induced by inflation of a balloon attached to the apex of a Miller-Abbott double lumen tube. DT tended to show decrease, while ST a slight increase, when the pain was evoked. However, in these pain induced experiments, ST changes were not so remarkable as those of ESR. A viscero-cutaneous reflex machanism and the predominance of sympathetic nerve control might be possible causes to produce these changes. Several important factors influencing the determination of the ESR were also discussed.", "PMID": 964022}
{"id": "pubmed23n0032_5772", "title": "Minute gastric cancer.", "content": "Nineteen cases of minute early gastric cancer were diagnosed under endoscopical examinations. Diagnosis was established by endoscopic biopsy in 18 cases, and exceptional one case of type IIb was incidentally found. Some of the endoscopic characteristic features of superficial depressed minute cancer were mentioned. By these findings, the diagnosis of superficial depressed minute cancer is possibly made in certain extent only by endoscopic observation. Prognosis of minute gastric cancer is excellent. Especially in the minute early cancer, the actual 7-year-survival rate was 100%. Prognosis of minute advanced cancer is also quite good. Minute gastric cancers can be found clinically and are almost completely curable.", "contents": "Minute gastric cancer. Nineteen cases of minute early gastric cancer were diagnosed under endoscopical examinations. Diagnosis was established by endoscopic biopsy in 18 cases, and exceptional one case of type IIb was incidentally found. Some of the endoscopic characteristic features of superficial depressed minute cancer were mentioned. By these findings, the diagnosis of superficial depressed minute cancer is possibly made in certain extent only by endoscopic observation. Prognosis of minute gastric cancer is excellent. Especially in the minute early cancer, the actual 7-year-survival rate was 100%. Prognosis of minute advanced cancer is also quite good. Minute gastric cancers can be found clinically and are almost completely curable.", "PMID": 964023}
{"id": "pubmed23n0032_5773", "title": "Clinical significance of Congo red test.", "content": "Clinical significance of Congo red test was studied, especially on its relation to the liver function and amyloidosis. No significant relationship was observed between Congo red test and Indocyanin green test in cases of various liver diseases except liver cirrhosis. Histological studies also revealed that there were no pathologic features specifically affecting Congo red index (CRI), even though CRI in cases of various liver diseases tended to be increased as compared with other diseases. As a cause of increased CRI which was seen most remarkably in cases of liver cirrhosis, obstructive change of the liver blood flow followed by the hepatic tissue damage is suspected. The concept that Congo red test is a kind of the hepatic excretory function test does not have, from our data, enough evidence. The present study confirmed our previous data on the fate of Congo red injected intravenously, which indicated that this test is one of the best tests available for RES function and this test is related mainly to Kupffer's cell rather than to the so-called hepatic excretory function. There were no cases which showed notable decrease of CRI in experimental amyloidosis and in clinical amyloidosis, and it can be said that Congo red test is not the best test for the diagnosis of amyloidosis.", "contents": "Clinical significance of Congo red test. Clinical significance of Congo red test was studied, especially on its relation to the liver function and amyloidosis. No significant relationship was observed between Congo red test and Indocyanin green test in cases of various liver diseases except liver cirrhosis. Histological studies also revealed that there were no pathologic features specifically affecting Congo red index (CRI), even though CRI in cases of various liver diseases tended to be increased as compared with other diseases. As a cause of increased CRI which was seen most remarkably in cases of liver cirrhosis, obstructive change of the liver blood flow followed by the hepatic tissue damage is suspected. The concept that Congo red test is a kind of the hepatic excretory function test does not have, from our data, enough evidence. The present study confirmed our previous data on the fate of Congo red injected intravenously, which indicated that this test is one of the best tests available for RES function and this test is related mainly to Kupffer's cell rather than to the so-called hepatic excretory function. There were no cases which showed notable decrease of CRI in experimental amyloidosis and in clinical amyloidosis, and it can be said that Congo red test is not the best test for the diagnosis of amyloidosis.", "PMID": 964024}
{"id": "pubmed23n0032_5774", "title": "Erythrocyte rosette forming activity of macrophage.", "content": "Erythrocyte rosette forming activities of the rat lung macrophage, peritoneal macrophage, Kupffer cells and expressed splenic cells were studied. The erythrocytes employed were fresh human red blood cell (RBC), fresh sheep RBC (SRBC), and tanned SRBC coated with bovine albumin or with bovine gamma-globulin. The macrophages were mixed with RBC at a ratio of 1 to 100 or 200 in gelatin veronal buffer or phosphate buffered saline. The experiments were carried out at 4 degrees C. No autologous serum nor anti-RBC serum was added to the experimental system. 10 to 20 per cent of the lung macrophages formed rosette and their entire surfaces were covered with RBC. The peritoneal macrophage and Kupffer cells were also capable of forming rosette with fresh SRBC as well as with SRBC coated with bovine albumin or with bovine gamma-globulin. Examinations for cell surface immunoglobulin of alveolar macrophage, peritoneal macrophage, Kupffer cells and splenic cells revealed that alveolar and peritoneal macrophages apparently possessed surface gamma-globulin and that almost all of these macrophages possessed surface immuno-globulin. In order to examine the effect of different natures of SRBC on in vivo phagocytosis, rats were immunized with SRBC by injecting various SRBC into the femoral or the portal vein and the hemagglutinin titer was studied. The maximum hemagglutinin titer was observed in the rat injected with fresh SRBC into the femoral vein and the minimum hemagglutinin titer was observed in the splenectomized rat injected with fresh SRBC and in the rat injected with SRBC coated with albumin or gamma-globulin into the femoral or portal vein. Immunological role of Kupffer cells was discussed.", "contents": "Erythrocyte rosette forming activity of macrophage. Erythrocyte rosette forming activities of the rat lung macrophage, peritoneal macrophage, Kupffer cells and expressed splenic cells were studied. The erythrocytes employed were fresh human red blood cell (RBC), fresh sheep RBC (SRBC), and tanned SRBC coated with bovine albumin or with bovine gamma-globulin. The macrophages were mixed with RBC at a ratio of 1 to 100 or 200 in gelatin veronal buffer or phosphate buffered saline. The experiments were carried out at 4 degrees C. No autologous serum nor anti-RBC serum was added to the experimental system. 10 to 20 per cent of the lung macrophages formed rosette and their entire surfaces were covered with RBC. The peritoneal macrophage and Kupffer cells were also capable of forming rosette with fresh SRBC as well as with SRBC coated with bovine albumin or with bovine gamma-globulin. Examinations for cell surface immunoglobulin of alveolar macrophage, peritoneal macrophage, Kupffer cells and splenic cells revealed that alveolar and peritoneal macrophages apparently possessed surface gamma-globulin and that almost all of these macrophages possessed surface immuno-globulin. In order to examine the effect of different natures of SRBC on in vivo phagocytosis, rats were immunized with SRBC by injecting various SRBC into the femoral or the portal vein and the hemagglutinin titer was studied. The maximum hemagglutinin titer was observed in the rat injected with fresh SRBC into the femoral vein and the minimum hemagglutinin titer was observed in the splenectomized rat injected with fresh SRBC and in the rat injected with SRBC coated with albumin or gamma-globulin into the femoral or portal vein. Immunological role of Kupffer cells was discussed.", "PMID": 964025}
{"id": "pubmed23n0032_5775", "title": "Long-term corticosteroid therapy of idiopathic aplastic anemia.", "content": "The results of a long-term corticosteroid therapy performed in 55 patients with idiopathic aplastic anemia are reported. Of these patients, cure was attained in 20 patients, remission in 5, and improvement in 10. The duration of the treatment of cured cases ranged from 138 to 1109 days with a median of 522 days. The results were much better than those with other treatments conventionally performed. However, necessity of paying attention to side-effects was pointed out.", "contents": "Long-term corticosteroid therapy of idiopathic aplastic anemia. The results of a long-term corticosteroid therapy performed in 55 patients with idiopathic aplastic anemia are reported. Of these patients, cure was attained in 20 patients, remission in 5, and improvement in 10. The duration of the treatment of cured cases ranged from 138 to 1109 days with a median of 522 days. The results were much better than those with other treatments conventionally performed. However, necessity of paying attention to side-effects was pointed out.", "PMID": 964026}
{"id": "pubmed23n0032_5776", "title": "Hemorrhagic diathesis in Weber-Christian disease.", "content": "A case of a 21-year-old woman with Weber-Christian disease, associated with consumption coagulopathy and adequately controlled over the past year or more by corticosteroid and heparin therapy, has been described. Through the study on this case, it was concluded that a hemorrhagic diathesis complicating Weber-Christian disease, at least in advanced cases, was characterized by consumption coagulopathy associated with enhanced blood coagulability which arises from hyperlipemia due to abnormal lipid metabolism and further, that the consumption coagulopathy was modified by diminished synthesis of clotting factors and activation of the fibrinolytic system due to the secondary hepatic impairment. It might also be likely that systemic vascular changes contribute to the activation of the clotting system as a trigger of clotting factor consumption. The complex pathophysiology of a hemorrhagic diathesis in this disease seemed to be brought about by a complex combination of such a variety of factors. In addition, decreased erythrocyte sedimentation rate observed characteristically during hemorrhage might probably have a direct correlation with hypofibrinogenemia due to consumption coagulopathy in advanced cases.", "contents": "Hemorrhagic diathesis in Weber-Christian disease. A case of a 21-year-old woman with Weber-Christian disease, associated with consumption coagulopathy and adequately controlled over the past year or more by corticosteroid and heparin therapy, has been described. Through the study on this case, it was concluded that a hemorrhagic diathesis complicating Weber-Christian disease, at least in advanced cases, was characterized by consumption coagulopathy associated with enhanced blood coagulability which arises from hyperlipemia due to abnormal lipid metabolism and further, that the consumption coagulopathy was modified by diminished synthesis of clotting factors and activation of the fibrinolytic system due to the secondary hepatic impairment. It might also be likely that systemic vascular changes contribute to the activation of the clotting system as a trigger of clotting factor consumption. The complex pathophysiology of a hemorrhagic diathesis in this disease seemed to be brought about by a complex combination of such a variety of factors. In addition, decreased erythrocyte sedimentation rate observed characteristically during hemorrhage might probably have a direct correlation with hypofibrinogenemia due to consumption coagulopathy in advanced cases.", "PMID": 964027}
{"id": "pubmed23n0032_5777", "title": "Histo-clinical classification and follow-up study of gastric polyp.", "content": "Gastric polyp was found in 1,616 cases or 0.23% of 711,455 persons through the gastric mass-survey. They were classified into 3 groups: hyperplastic polyp in 61%, gastric polyposa in 34%, and polyp composed of metaplastic epithelium with marked atypism (ATP) in 5%. Gastritis polyposa was subclassified into 5 groups according to its histo-clinical features. Malignant degeneration was suspected in 5 or 2.1% of 236 hyperplastic polyps operated, while no polyp which had changed into cancer was experienced among 974 polyps followed-up by biopsy during the time course of 6 months to 11 years at the longest. On the other hand, coexistence of gastric cancer was found in 13% of cases with ATP. In polyps composed of metaplastic epithelium, there are two kinds of polyp: metaplastic polyp in hyperplastic proliferation and neoplastic polyp. In the follow-up study of 1,104 polyps, the growth of polyp was seen in only 14, and 12 of which were hyperplastic polyp. No change from gastritis polyposa to hyperplastic polyp was observed. The increase in size in 14 polyps was seen mostly 3 to 4 years after they were found and was always transient. From these results, it is considered that most of gastric polyps clinically detected have already completed their growth.", "contents": "Histo-clinical classification and follow-up study of gastric polyp. Gastric polyp was found in 1,616 cases or 0.23% of 711,455 persons through the gastric mass-survey. They were classified into 3 groups: hyperplastic polyp in 61%, gastric polyposa in 34%, and polyp composed of metaplastic epithelium with marked atypism (ATP) in 5%. Gastritis polyposa was subclassified into 5 groups according to its histo-clinical features. Malignant degeneration was suspected in 5 or 2.1% of 236 hyperplastic polyps operated, while no polyp which had changed into cancer was experienced among 974 polyps followed-up by biopsy during the time course of 6 months to 11 years at the longest. On the other hand, coexistence of gastric cancer was found in 13% of cases with ATP. In polyps composed of metaplastic epithelium, there are two kinds of polyp: metaplastic polyp in hyperplastic proliferation and neoplastic polyp. In the follow-up study of 1,104 polyps, the growth of polyp was seen in only 14, and 12 of which were hyperplastic polyp. No change from gastritis polyposa to hyperplastic polyp was observed. The increase in size in 14 polyps was seen mostly 3 to 4 years after they were found and was always transient. From these results, it is considered that most of gastric polyps clinically detected have already completed their growth.", "PMID": 964028}
{"id": "pubmed23n0032_5778", "title": "Fasting therapy for psychosomatic diseases with special reference to its indication and therapeutic mechanism.", "content": "It has been well known that the fasting therapy which was invented in Medical School of Tohoku University reveals an excellent effect upon various kinds of psychosomatic diseases, but its therapeutic mechanism and suitable indication are not yet explained completely. In order to corroborate these problems, this study was undertaken on 262 cases of psychosomatic diseases in the field of internal medicine. It is a complete fasting for 10 days with nothing by mouth except for drinking water, and 500 ml of parenteral fluid containing vitamins are administered intravenously every day. Absolute bed rest and self meditation are required in a closed individual room, and patients are not allowed to meet anyone but physicians and nurse in charge. The return to normal ordinary diet follows the order of fluid diet, soft diet and semiordinary diet during 5 days. In the period of the therapy, various clinical and laboratory examinations were carried out. Significance of these examinations consists in prediction of possible danger during the fasting period and elucidation of its therapeutic mechanism. Consequently, an outstanding efficacy rate of 87% with excellent prognosis was attained, and the following diseases were determined as suitable indication of this therapy; irritable colon, dysorexia nervosa, borderline hypertension, neurocirculatory asthenia, bronchial asthma, mild diabetes mellitus, obesity, lumbago without organic findings, conversion hysteria, various neurosis with somatic symptoms and masked depression. Possible mechanism of action of the therapy is that fasting acts as an extreme stress on the function of the autonomic nervous and endocrine systems, then it regulates the function of whole body including the brain, also it acts as one of the behaviour therapy for abnormal conditioning.", "contents": "Fasting therapy for psychosomatic diseases with special reference to its indication and therapeutic mechanism. It has been well known that the fasting therapy which was invented in Medical School of Tohoku University reveals an excellent effect upon various kinds of psychosomatic diseases, but its therapeutic mechanism and suitable indication are not yet explained completely. In order to corroborate these problems, this study was undertaken on 262 cases of psychosomatic diseases in the field of internal medicine. It is a complete fasting for 10 days with nothing by mouth except for drinking water, and 500 ml of parenteral fluid containing vitamins are administered intravenously every day. Absolute bed rest and self meditation are required in a closed individual room, and patients are not allowed to meet anyone but physicians and nurse in charge. The return to normal ordinary diet follows the order of fluid diet, soft diet and semiordinary diet during 5 days. In the period of the therapy, various clinical and laboratory examinations were carried out. Significance of these examinations consists in prediction of possible danger during the fasting period and elucidation of its therapeutic mechanism. Consequently, an outstanding efficacy rate of 87% with excellent prognosis was attained, and the following diseases were determined as suitable indication of this therapy; irritable colon, dysorexia nervosa, borderline hypertension, neurocirculatory asthenia, bronchial asthma, mild diabetes mellitus, obesity, lumbago without organic findings, conversion hysteria, various neurosis with somatic symptoms and masked depression. Possible mechanism of action of the therapy is that fasting acts as an extreme stress on the function of the autonomic nervous and endocrine systems, then it regulates the function of whole body including the brain, also it acts as one of the behaviour therapy for abnormal conditioning.", "PMID": 964029}
{"id": "pubmed23n0032_5779", "title": "Role of the kidney in gastrin metabolism.", "content": "Fasting blood gastrin level among various clinical entities was measured by means of radioimmunoassay. Hypergastrinemia of various degrees was found in some cases of chronic renal failure. During angiography arteriovenous difference of gastrin level was investigated in the kidney in 22 patients without renal diseases. The high rate of gastrin removal from the blood circulation by the kidney was also confirmed in the synthetic human gastrin 1-17 (SHG 1-17) infusion experiment in dogs. In this study, hypergastrinemia seen in renal diseases was thought to be caused partly by impaired gastrin metabolism in the kidney.", "contents": "Role of the kidney in gastrin metabolism. Fasting blood gastrin level among various clinical entities was measured by means of radioimmunoassay. Hypergastrinemia of various degrees was found in some cases of chronic renal failure. During angiography arteriovenous difference of gastrin level was investigated in the kidney in 22 patients without renal diseases. The high rate of gastrin removal from the blood circulation by the kidney was also confirmed in the synthetic human gastrin 1-17 (SHG 1-17) infusion experiment in dogs. In this study, hypergastrinemia seen in renal diseases was thought to be caused partly by impaired gastrin metabolism in the kidney.", "PMID": 964030}
{"id": "pubmed23n0032_5780", "title": "Endoscopical polypectomy of the gastric polyp with electro-coagulation wire loop.", "content": "A forward-viewing fiberscope equipped with two channels was designed for safer and easier gastric polypectomy under endoscopic control using electro-surgical technique. No electrical accident, bleeding, perforation or significant complications were noticed. Mucosal ulcerations at the polypectomy site healed within a month leaving minor scars behind. In 30 cases, U1 II gastric ulcers developed after polypectomy, which healed leaving convergence of mucosal folds behind. Fiberscopic excision of gastric polyps was also useful for establishing a reliable diagnosis of gastric polyp cancer which is sometimes overlooked by routine biopsy procedure. It may be the \"perfect biopsy\". The patient with gastric polyp who is aware of his own illness and feels uneasy can be released from his anxiety and regain both physical and mental health at the cost of minimal risks of endoscopic polypectomy.", "contents": "Endoscopical polypectomy of the gastric polyp with electro-coagulation wire loop. A forward-viewing fiberscope equipped with two channels was designed for safer and easier gastric polypectomy under endoscopic control using electro-surgical technique. No electrical accident, bleeding, perforation or significant complications were noticed. Mucosal ulcerations at the polypectomy site healed within a month leaving minor scars behind. In 30 cases, U1 II gastric ulcers developed after polypectomy, which healed leaving convergence of mucosal folds behind. Fiberscopic excision of gastric polyps was also useful for establishing a reliable diagnosis of gastric polyp cancer which is sometimes overlooked by routine biopsy procedure. It may be the \"perfect biopsy\". The patient with gastric polyp who is aware of his own illness and feels uneasy can be released from his anxiety and regain both physical and mental health at the cost of minimal risks of endoscopic polypectomy.", "PMID": 964031}
{"id": "pubmed23n0032_5781", "title": "Evaluation of mass screening program for stomach cancer.", "content": "Particular method and program of mass survey examination of gastric cancer were introduced into Miyagi Prefecture and the results accumulated during the past 14 years are presented. During this period, 1,427 cases of gastric cancer (0.18%), as well as many cases of other diseases of the stomach and duodenum, were found by the mass survey. 450 cases were those of early cancer in which invasion of carcinoma was limited to the mucosa and submucosa. The ratio of surgically confirmed early cancer cases to all the stomach cancer cases was 36.4%. Almost in all cases of early stomach cancer there were neither complaints nor clinical symptoms; that is, they were the cases of so-called preclinical cancer of the stomach. The prognosis after surgery of early stomach cancer was remarkably favorable with a five-year survival rate over 90% and the death rate due to stomach cancer was actually decreased in the surveyed population.", "contents": "Evaluation of mass screening program for stomach cancer. Particular method and program of mass survey examination of gastric cancer were introduced into Miyagi Prefecture and the results accumulated during the past 14 years are presented. During this period, 1,427 cases of gastric cancer (0.18%), as well as many cases of other diseases of the stomach and duodenum, were found by the mass survey. 450 cases were those of early cancer in which invasion of carcinoma was limited to the mucosa and submucosa. The ratio of surgically confirmed early cancer cases to all the stomach cancer cases was 36.4%. Almost in all cases of early stomach cancer there were neither complaints nor clinical symptoms; that is, they were the cases of so-called preclinical cancer of the stomach. The prognosis after surgery of early stomach cancer was remarkably favorable with a five-year survival rate over 90% and the death rate due to stomach cancer was actually decreased in the surveyed population.", "PMID": 964032}
{"id": "pubmed23n0032_5782", "title": "Extraventricular lesions demonstrated by gastric photofluorographic study.", "content": "In a mass survey for diseases of the stomach with photofluography, lesions other than those of the stomach were studied in 85,040 subjects. Gastric lesions were detected in 2.17%, while other lesions were found in 1.97%, giving an approximately similar ratio. Duodenal lesions were found most frequently and in 1.47%, followed by intraabdominal calcification in 0.44%. Among duodenal lesions, duodenal ulcer was seen in 0.14%, and duodenal diverticulum in 0.34%. Among diseases with abdominal calcifications, calcification of lymph nodes was found most frequently and in 0.24%, followed by cholelithiasis in 0.085% and urolithiasis in 0.004%. Lower esophageal lesions were found in 0.05%, in which esophageal diverticulum was the most frequent and found in 0.034%.", "contents": "Extraventricular lesions demonstrated by gastric photofluorographic study. In a mass survey for diseases of the stomach with photofluography, lesions other than those of the stomach were studied in 85,040 subjects. Gastric lesions were detected in 2.17%, while other lesions were found in 1.97%, giving an approximately similar ratio. Duodenal lesions were found most frequently and in 1.47%, followed by intraabdominal calcification in 0.44%. Among duodenal lesions, duodenal ulcer was seen in 0.14%, and duodenal diverticulum in 0.34%. Among diseases with abdominal calcifications, calcification of lymph nodes was found most frequently and in 0.24%, followed by cholelithiasis in 0.085% and urolithiasis in 0.004%. Lower esophageal lesions were found in 0.05%, in which esophageal diverticulum was the most frequent and found in 0.034%.", "PMID": 964033}
{"id": "pubmed23n0032_5783", "title": "Relationship between disturbances of micellar formation and steatorrhea.", "content": "In order to clarify the correlation between decrease in micellar formation and degree of steatorrhea, upper intestinal content was analyzed after administering a test meal of Borgstr\u00f6m et al. Fecal fat was also measured. It was demonstrated that micellar phase was composed chiefly of fatty acid and monoglyceride, with low concentrations of diglyceride and triglyceride. The fat composition of micellar phase was nearly constant in normal subjects and in cases of malabsorption syndrome. The correlation between the ratio of micellar fat to the whole ingested fat in the intestinal content and daily excretion of fecal fat was very marked.", "contents": "Relationship between disturbances of micellar formation and steatorrhea. In order to clarify the correlation between decrease in micellar formation and degree of steatorrhea, upper intestinal content was analyzed after administering a test meal of Borgstr\u00f6m et al. Fecal fat was also measured. It was demonstrated that micellar phase was composed chiefly of fatty acid and monoglyceride, with low concentrations of diglyceride and triglyceride. The fat composition of micellar phase was nearly constant in normal subjects and in cases of malabsorption syndrome. The correlation between the ratio of micellar fat to the whole ingested fat in the intestinal content and daily excretion of fecal fat was very marked.", "PMID": 964034}
{"id": "pubmed23n0032_5784", "title": "Abnormal micelle formation in various malabsorption syndromes.", "content": "In order to clarify the abnormal micelle formation in various malabsorption syndromes, the analysis of upper intestinal contents after administering a test meal of Borgstr\u00f6m et al. was performed. It was demonstrated that the percentage of micellar fat to total fat and lipase concentrations decreased markedly from the normal controls despite of normal concentrations of bile salts and pH in cases of pancreatitis and pancreatectomy. In contrast, it was shown that the percentage of micellar fat was markedly low and the concentrations of conjugated bile salts were reduced in cases of ileal diseases. Unconjugated bile salts were detected although the concentrations of total bile salts were normal in blind-loop syndrome. The correlation between the percentage of micellar fat and lipase concentrations or bile salts concentrations in normal controls and various malabsorption syndromes was also reported.", "contents": "Abnormal micelle formation in various malabsorption syndromes. In order to clarify the abnormal micelle formation in various malabsorption syndromes, the analysis of upper intestinal contents after administering a test meal of Borgstr\u00f6m et al. was performed. It was demonstrated that the percentage of micellar fat to total fat and lipase concentrations decreased markedly from the normal controls despite of normal concentrations of bile salts and pH in cases of pancreatitis and pancreatectomy. In contrast, it was shown that the percentage of micellar fat was markedly low and the concentrations of conjugated bile salts were reduced in cases of ileal diseases. Unconjugated bile salts were detected although the concentrations of total bile salts were normal in blind-loop syndrome. The correlation between the percentage of micellar fat and lipase concentrations or bile salts concentrations in normal controls and various malabsorption syndromes was also reported.", "PMID": 964035}
{"id": "pubmed23n0032_5785", "title": "Cytological diagnoses of early gastric cancer.", "content": "To evaluate the clinical value of gastric cytology in the detection of stomach cancer, the results of cytological examinations in 417 cases of early cancer of the stomach in which cancer cell infiltration was limited to the mucosa or submucosa were analyzed. The cytological examination revealed positive for cancer cells in 93% of these cases, and therefore it is concluded that this method is not only useful but indispensable for the diagnosis of early cancer of the stomach. The gastric cytology proved to be more reliable than the endoscopic and X-ray examinations in terms of detection rate of the early gastric cancer. Among various cell collection methods for gastric cytology, which include the washing, abrasive and fibergastroscopic methods, the washing method under direct vision by the use of fiberscope showed the highest value of positivity (92.8%) in the cases of early cancer of the stomach. With the help of endoscopic guidance and biopsy techniques, the cytological examination further increased its reliability and ability in the detection of early gastric cancer. Cancer lesions even smaller than 1 cm can now be correctly diagnosed by the gastric cytology.", "contents": "Cytological diagnoses of early gastric cancer. To evaluate the clinical value of gastric cytology in the detection of stomach cancer, the results of cytological examinations in 417 cases of early cancer of the stomach in which cancer cell infiltration was limited to the mucosa or submucosa were analyzed. The cytological examination revealed positive for cancer cells in 93% of these cases, and therefore it is concluded that this method is not only useful but indispensable for the diagnosis of early cancer of the stomach. The gastric cytology proved to be more reliable than the endoscopic and X-ray examinations in terms of detection rate of the early gastric cancer. Among various cell collection methods for gastric cytology, which include the washing, abrasive and fibergastroscopic methods, the washing method under direct vision by the use of fiberscope showed the highest value of positivity (92.8%) in the cases of early cancer of the stomach. With the help of endoscopic guidance and biopsy techniques, the cytological examination further increased its reliability and ability in the detection of early gastric cancer. Cancer lesions even smaller than 1 cm can now be correctly diagnosed by the gastric cytology.", "PMID": 964036}
{"id": "pubmed23n0032_5786", "title": "Crohn's disease, non-specific ulcers of the small intestine, and idiopathic proctocolitis in a Japanese university hospital from 1954 to 1974.", "content": "Most of the cases of Crohn's disease reported in Japan were originally treated surgically as acute appendicitis and, after appendectomy, they were diagnosed as acute terminal ileitis or acute Crohn's disease, which should belong to a category different from typical Crohn's disease, according to the international nomenclature by the Council for International Organization of Medical Sciences in 1973. Reviewing our university hospital records from 1954 to 1974, the incidence of typical Crohn's disease and idiopathic proctocolitis has been increasing, while the patients with intestinal tuberculosis have been decreasing. Clinical and histopathological features of operated three groups of our patients with Crohn's disease of the small intestine, non-specific ulcers of the small intestine and prestomal ileitis were comparatively studied. Furthermore, 9 cases of operated Crohn's disease of the colon and 23 cases of operated idiopathic proctocolitis were similarly evaluated. The importance of diagnosing Crohn's disease as a whole from both clinical and histopathological stand points of view was emphasized, and main differential diagnostic criteria between Crohn's disease and idiopathic proctocolitis were discussed.", "contents": "Crohn's disease, non-specific ulcers of the small intestine, and idiopathic proctocolitis in a Japanese university hospital from 1954 to 1974. Most of the cases of Crohn's disease reported in Japan were originally treated surgically as acute appendicitis and, after appendectomy, they were diagnosed as acute terminal ileitis or acute Crohn's disease, which should belong to a category different from typical Crohn's disease, according to the international nomenclature by the Council for International Organization of Medical Sciences in 1973. Reviewing our university hospital records from 1954 to 1974, the incidence of typical Crohn's disease and idiopathic proctocolitis has been increasing, while the patients with intestinal tuberculosis have been decreasing. Clinical and histopathological features of operated three groups of our patients with Crohn's disease of the small intestine, non-specific ulcers of the small intestine and prestomal ileitis were comparatively studied. Furthermore, 9 cases of operated Crohn's disease of the colon and 23 cases of operated idiopathic proctocolitis were similarly evaluated. The importance of diagnosing Crohn's disease as a whole from both clinical and histopathological stand points of view was emphasized, and main differential diagnostic criteria between Crohn's disease and idiopathic proctocolitis were discussed.", "PMID": 964037}
{"id": "pubmed23n0032_5787", "title": "[Effectiveness of antibiotic premedication in colonic surgery].", "content": "The value of preoperative bowel preparation with antibiotics in colon surgery was studied in a prospective randomized trial: 58 patients received only mechanical and dietary preparation and 50 patients were treated additionally with a combination of neomycin and bacitracin 4 days preoperatively. Bacteriologic studies of stools from the day before beginning of preparation and from the day before surgery which were done quantitatively per 1 gram stool revealed that the fecal flora of 90% of the patients with colonic disease is abnormal. After antibiotic treatment a significant reduction of Escherichia coli, Bacteroides, Bifidus, Clostridium, and Proteus was obtained in more than 70% of the patients. The effect on Klebsiella was poor. Candida were increased in 86% of the cases without resulting in a systemic candida infection. In the stool samples of 16 patients no bacteria were found after antibiotic treatment. If there was a high bacterial count in the preoperative stool infectious complications followed postoperatively. The rate of infectious complications was 28 (48.7%) in the control group and 7 (14%) in the antibiotic group. This significant lower rate of complications speaks in favor of preoperative bowel preparation with neomycin and bacitracin, especially in the absence of adverse side-effects.", "contents": "[Effectiveness of antibiotic premedication in colonic surgery]. The value of preoperative bowel preparation with antibiotics in colon surgery was studied in a prospective randomized trial: 58 patients received only mechanical and dietary preparation and 50 patients were treated additionally with a combination of neomycin and bacitracin 4 days preoperatively. Bacteriologic studies of stools from the day before beginning of preparation and from the day before surgery which were done quantitatively per 1 gram stool revealed that the fecal flora of 90% of the patients with colonic disease is abnormal. After antibiotic treatment a significant reduction of Escherichia coli, Bacteroides, Bifidus, Clostridium, and Proteus was obtained in more than 70% of the patients. The effect on Klebsiella was poor. Candida were increased in 86% of the cases without resulting in a systemic candida infection. In the stool samples of 16 patients no bacteria were found after antibiotic treatment. If there was a high bacterial count in the preoperative stool infectious complications followed postoperatively. The rate of infectious complications was 28 (48.7%) in the control group and 7 (14%) in the antibiotic group. This significant lower rate of complications speaks in favor of preoperative bowel preparation with neomycin and bacitracin, especially in the absence of adverse side-effects.", "PMID": 964056}
{"id": "pubmed23n0032_5788", "title": "[2 years of experience with balanced oral feeding in abdominal surgery].", "content": "Together with the progress of surgical technique there is the continued development of pre- and postoperative care of patients. There are two fields for the use of BSD (balanced synthetic diet): I. Preoperative: a) Full caloric nutrition b) Intestines free from waste matter and lowering of bacterial content. II. Postoperative: Interruption of the vicious circle of diminished offer of nutrition, postoperative katabolism, which increases the need for amino-acids and so interferes with the process of wound-healing and the protection against infection.", "contents": "[2 years of experience with balanced oral feeding in abdominal surgery]. Together with the progress of surgical technique there is the continued development of pre- and postoperative care of patients. There are two fields for the use of BSD (balanced synthetic diet): I. Preoperative: a) Full caloric nutrition b) Intestines free from waste matter and lowering of bacterial content. II. Postoperative: Interruption of the vicious circle of diminished offer of nutrition, postoperative katabolism, which increases the need for amino-acids and so interferes with the process of wound-healing and the protection against infection.", "PMID": 964057}
{"id": "pubmed23n0032_5789", "title": "[Physical flow studies of new surgical aspiration drains].", "content": "Mathematical and experimental results demonstrate that the customary Redon drain as well as the improved Neo-Redovac drain develop suction only over a short initial distance. The above mentioned innovation of three additional oval holes does not increase efficiency. The disadvantage of rapidly diminished suction is overcome in the \"Ulmer Drain\", in which equidistant openings of continually increasing aperture are aligned towards the drain end.", "contents": "[Physical flow studies of new surgical aspiration drains]. Mathematical and experimental results demonstrate that the customary Redon drain as well as the improved Neo-Redovac drain develop suction only over a short initial distance. The above mentioned innovation of three additional oval holes does not increase efficiency. The disadvantage of rapidly diminished suction is overcome in the \"Ulmer Drain\", in which equidistant openings of continually increasing aperture are aligned towards the drain end.", "PMID": 964058}
{"id": "pubmed23n0032_5790", "title": "[The so-called spontaneous nodular panniculitis (Weber-Christian lipodystrophy)].", "content": "Enlarged lymph nodes and a tumor, the size of a man's fist, were found in the abdomen of a female patient of 63. Histologic diagnosis determined a non-suppurative panniculitis with typical proliferation of foam cells and reactive inflammatory infiltration attaining the stage of granulomatosis. The disease is seen as granulomatous histiocytosis; a sure explanation of its pathogenesis is not yet possible.", "contents": "[The so-called spontaneous nodular panniculitis (Weber-Christian lipodystrophy)]. Enlarged lymph nodes and a tumor, the size of a man's fist, were found in the abdomen of a female patient of 63. Histologic diagnosis determined a non-suppurative panniculitis with typical proliferation of foam cells and reactive inflammatory infiltration attaining the stage of granulomatosis. The disease is seen as granulomatous histiocytosis; a sure explanation of its pathogenesis is not yet possible.", "PMID": 964059}
{"id": "pubmed23n0032_5791", "title": "[The programmed medical report in surgery].", "content": "Our experience in programs for semi-automatic medical reports developed and used in the course of the last 4 years was as follows: 9 programs for inpatients and 3 for outpatients have been developed. Programs for semi-automatic medical reports have proved especially advantageous in frequent diseases (as for example appendicitis, cholelithiasis) and in managing large special consulting hours for outpatients with predominantly similar diseases. Due to rational and cheap work the use of semi-automatic medical reports in a certain range shows an alternative to the conventional ways of writing from dictation.", "contents": "[The programmed medical report in surgery]. Our experience in programs for semi-automatic medical reports developed and used in the course of the last 4 years was as follows: 9 programs for inpatients and 3 for outpatients have been developed. Programs for semi-automatic medical reports have proved especially advantageous in frequent diseases (as for example appendicitis, cholelithiasis) and in managing large special consulting hours for outpatients with predominantly similar diseases. Due to rational and cheap work the use of semi-automatic medical reports in a certain range shows an alternative to the conventional ways of writing from dictation.", "PMID": 964062}
{"id": "pubmed23n0032_5792", "title": "[X-ray archivation with microfilm].", "content": "The LogEtronics X-Ray Microfilms system, which is used in our clinic as well as in two other Berufsgenossenschaftliche Unfallkliniken for archiving of x-ray films, has enabled us to microfilm all x-rays of 4 years within 14 months. The conception of the microfilm system has also stood the test from the point of view of fulfilling all legal requirements.", "contents": "[X-ray archivation with microfilm]. The LogEtronics X-Ray Microfilms system, which is used in our clinic as well as in two other Berufsgenossenschaftliche Unfallkliniken for archiving of x-ray films, has enabled us to microfilm all x-rays of 4 years within 14 months. The conception of the microfilm system has also stood the test from the point of view of fulfilling all legal requirements.", "PMID": 964063}
{"id": "pubmed23n0032_5793", "title": "[Esophagopericardial fistula with tension pneumopericardium].", "content": "Reported on is a 72 year old man in whom a diverticulum of the lower esophagus had perforated into the pericardium causing pyopneumopericardium and cardiac tamponade during esophagoscopy. After resuscitation and drainage of the pericardial cavity the patient ameliorated and three weeks later the fistula between the esophagus and the pericardial sac had closed at least functionally as judged by a gastrografin-swallow. Planned definitive surgical closure of the fistula could not be performed because the patient's condition deteriorated following tracheobronchial aspiration. He succumbed due to bilateral pleuro-bronchopneumonia, progressive renal failure and central circulatory dysregulation. Reviewing 24 reported cases the clinical signs, etiology, differential diagnosis, therapeutic management and prognosis of this rare condition are discussed.", "contents": "[Esophagopericardial fistula with tension pneumopericardium]. Reported on is a 72 year old man in whom a diverticulum of the lower esophagus had perforated into the pericardium causing pyopneumopericardium and cardiac tamponade during esophagoscopy. After resuscitation and drainage of the pericardial cavity the patient ameliorated and three weeks later the fistula between the esophagus and the pericardial sac had closed at least functionally as judged by a gastrografin-swallow. Planned definitive surgical closure of the fistula could not be performed because the patient's condition deteriorated following tracheobronchial aspiration. He succumbed due to bilateral pleuro-bronchopneumonia, progressive renal failure and central circulatory dysregulation. Reviewing 24 reported cases the clinical signs, etiology, differential diagnosis, therapeutic management and prognosis of this rare condition are discussed.", "PMID": 964064}
{"id": "pubmed23n0032_5794", "title": "[Blunt renal trauma: diagnosis, therapy and possible complications].", "content": "223 cases of blunt renal injuries, which were observed over a period of 19 years at the department of Surgery, University of Graz, are presented in this study. The value of several diagnostic procedures and the therapy are discussed in respect of early and late complications and the literature reviewed.", "contents": "[Blunt renal trauma: diagnosis, therapy and possible complications]. 223 cases of blunt renal injuries, which were observed over a period of 19 years at the department of Surgery, University of Graz, are presented in this study. The value of several diagnostic procedures and the therapy are discussed in respect of early and late complications and the literature reviewed.", "PMID": 964065}
{"id": "pubmed23n0032_5795", "title": "[Solitary bone cysts in the foot].", "content": "The localisations of bone cysts known under the title of \"intraosseous ganglion, synovial cyst of bone and solitary unicameral cyst\" are summarised from the literature. Two of the authors' patients are added to the few cases of talar cysts recorded up to the present. In addition, a patient with a calcaneal cyst is described. The cysts can be traumatic or non-traumatic in origin. The various theories concerning their etiology are discussed. The clinical findings, differential diagnosis and therapy of subchondral bone cysts particularly in the region of the foot are considered.", "contents": "[Solitary bone cysts in the foot]. The localisations of bone cysts known under the title of \"intraosseous ganglion, synovial cyst of bone and solitary unicameral cyst\" are summarised from the literature. Two of the authors' patients are added to the few cases of talar cysts recorded up to the present. In addition, a patient with a calcaneal cyst is described. The cysts can be traumatic or non-traumatic in origin. The various theories concerning their etiology are discussed. The clinical findings, differential diagnosis and therapy of subchondral bone cysts particularly in the region of the foot are considered.", "PMID": 964066}
{"id": "pubmed23n0032_5796", "title": "[Cultivation possibility for obligatory anaerobic bacteria from contaminated cotton carriers after storage. Comparative testing of simple swabs and swabs in transport medium using Stuart's method].", "content": "26 strains of obligate anaerobic bacteria, which had been isolated from clinical specimens, were tested for their survival on artificially contaminated cotton swabs after storage. All strains could hardly if at all be cultivated, if the cotton swabs had been stored in empty test tubes at 4 degrees C for 48 hrs. In contrast to that it was possible to cultivate all strains without significant reduction of their number from swabs, which were stored at 4 degrees C for 48 hrs in test tubes containing Stuart's transport medium. The necessity of an improvement of techniques and transport-methods to isolate anaerobic bacteria is discussed. The use of a special transport medium is recommended as an important aid to the early recognition of anaerobic infection.", "contents": "[Cultivation possibility for obligatory anaerobic bacteria from contaminated cotton carriers after storage. Comparative testing of simple swabs and swabs in transport medium using Stuart's method]. 26 strains of obligate anaerobic bacteria, which had been isolated from clinical specimens, were tested for their survival on artificially contaminated cotton swabs after storage. All strains could hardly if at all be cultivated, if the cotton swabs had been stored in empty test tubes at 4 degrees C for 48 hrs. In contrast to that it was possible to cultivate all strains without significant reduction of their number from swabs, which were stored at 4 degrees C for 48 hrs in test tubes containing Stuart's transport medium. The necessity of an improvement of techniques and transport-methods to isolate anaerobic bacteria is discussed. The use of a special transport medium is recommended as an important aid to the early recognition of anaerobic infection.", "PMID": 964067}
{"id": "pubmed23n0032_5797", "title": "[Wound infection following arterial surgery in the pelvis-leg region].", "content": "In a series of 581 reconstructions of the aorta to the leg arteries, 28 cases (4.8%) developed wound infection with positive microbiological identification. There were 24 monoinfections with significant prevalence of Staphylococcus aureus and S. epidermis. Thirteen patients with prosthetic implants and one patient with autologous saphenous vein bypass showed graft infection, which occurred in 13 patients as a complication of reoperation for bleeding or graft occlusion in the early postoperative period. The incidence of vascular infection in patients without reoperation was 0.4%. The risk of wound infection could not be lowered by the use of prophylactic broad spectrum antibiotics. Five (33%) patients with graft infection died because of sepsis and/or rupture of anastomosis. In the group of 9 survivors there were 3 patients with excision of the graft and limb preservation without reconstruction, and 3 patients with partial or total excision of the graft and successful simultaneous axillofemoral or obturator bypass. From this study it is assumed that improvement of indication and operative technique in reconstructive procedures is more promising in preventing wound infection than the extended administration of prophylactic antibiotic drugs. In case of vascular infection the excision of the graft is very urgent and consequent \"extraanatomic\" reconstruction can prevent loss of limb and life.", "contents": "[Wound infection following arterial surgery in the pelvis-leg region]. In a series of 581 reconstructions of the aorta to the leg arteries, 28 cases (4.8%) developed wound infection with positive microbiological identification. There were 24 monoinfections with significant prevalence of Staphylococcus aureus and S. epidermis. Thirteen patients with prosthetic implants and one patient with autologous saphenous vein bypass showed graft infection, which occurred in 13 patients as a complication of reoperation for bleeding or graft occlusion in the early postoperative period. The incidence of vascular infection in patients without reoperation was 0.4%. The risk of wound infection could not be lowered by the use of prophylactic broad spectrum antibiotics. Five (33%) patients with graft infection died because of sepsis and/or rupture of anastomosis. In the group of 9 survivors there were 3 patients with excision of the graft and limb preservation without reconstruction, and 3 patients with partial or total excision of the graft and successful simultaneous axillofemoral or obturator bypass. From this study it is assumed that improvement of indication and operative technique in reconstructive procedures is more promising in preventing wound infection than the extended administration of prophylactic antibiotic drugs. In case of vascular infection the excision of the graft is very urgent and consequent \"extraanatomic\" reconstruction can prevent loss of limb and life.", "PMID": 964072}
{"id": "pubmed23n0032_5798", "title": "[Spontaneous splenic rupture].", "content": "Discussion of rupture of the spleen apparently without cause; the patient suffered no trauma nor did he have a disease of the spleen. The conclusion is that the rupture occurred spontaneously.", "contents": "[Spontaneous splenic rupture]. Discussion of rupture of the spleen apparently without cause; the patient suffered no trauma nor did he have a disease of the spleen. The conclusion is that the rupture occurred spontaneously.", "PMID": 964073}
{"id": "pubmed23n0032_5799", "title": "[Ischemic necroses of the colon and rectum following alloplastic vessel substitution of the abdominal aorta].", "content": "Arterial insufficiency to the colon and rectum followed by ischemic necrosis of this bowel portion is considered to be a rare complication after resection of abdominal aortic aneurysms and alloplastic replacement of the abdominal aorta in arterial occlusive disease. The main symptom is diarrhea and mucus or blood in stool. Sigmoidoscopic examination is of diagnostic value; the treatment of choice has to be colostomy and resection. A causative factor for the development of large bowel necrosis is diminution of collateral blood supply, which is discussed in detail.", "contents": "[Ischemic necroses of the colon and rectum following alloplastic vessel substitution of the abdominal aorta]. Arterial insufficiency to the colon and rectum followed by ischemic necrosis of this bowel portion is considered to be a rare complication after resection of abdominal aortic aneurysms and alloplastic replacement of the abdominal aorta in arterial occlusive disease. The main symptom is diarrhea and mucus or blood in stool. Sigmoidoscopic examination is of diagnostic value; the treatment of choice has to be colostomy and resection. A causative factor for the development of large bowel necrosis is diminution of collateral blood supply, which is discussed in detail.", "PMID": 964081}
{"id": "pubmed23n0032_5800", "title": "[Lymph flow disorders following arterial surgery of the leg].", "content": "In a series of 342 arterial reconstructions of the iliacolic to popliteal arteries 24 patients with lymph vessel damage were observed. The diagnosis of lymphedema was established in 18 patients by clinical findings and in 2 of them the diagnosis was confirmed by lymphography with followup of 1 year. Three patients developed an internal lymphogenic cyst, 3 showed an external lymph fistula, and 1 patient developed an infection of a dacron bifurcation graft. The infection occurred when an additional occlusion of the left femoral artery was bypassed with an autologous saphenous vein graft and a lymph cyst in the groin, which was excised several times, became infected. The patient was treated successfully by excising the graft and performing an axillofemoral bypass. Although cases of surgical damage to lymph vessels following arterial reconstructions are rare in the literature, it is assumed that this complication occurs more often than reported, because of lack of lymphographic information.", "contents": "[Lymph flow disorders following arterial surgery of the leg]. In a series of 342 arterial reconstructions of the iliacolic to popliteal arteries 24 patients with lymph vessel damage were observed. The diagnosis of lymphedema was established in 18 patients by clinical findings and in 2 of them the diagnosis was confirmed by lymphography with followup of 1 year. Three patients developed an internal lymphogenic cyst, 3 showed an external lymph fistula, and 1 patient developed an infection of a dacron bifurcation graft. The infection occurred when an additional occlusion of the left femoral artery was bypassed with an autologous saphenous vein graft and a lymph cyst in the groin, which was excised several times, became infected. The patient was treated successfully by excising the graft and performing an axillofemoral bypass. Although cases of surgical damage to lymph vessels following arterial reconstructions are rare in the literature, it is assumed that this complication occurs more often than reported, because of lack of lymphographic information.", "PMID": 964082}
{"id": "pubmed23n0032_5801", "title": "[Leiomyoma of the portal vein].", "content": "A case report of a 34-year-old female with leiomyoma of the portal vein is presented. The tumor was found accidentally, when laparatomy with diagnosis of cholelithiasis was performed. Total excision of the leiomyoma including a part of the anterior wall of the portal vein was necessary and could be carried out. The continuity of the portal vein could be preserved using continuous vascular suture. Microscopical examination of the tumor showed an increased proliferation rate in the leiomyoma; therefore the tumor was classified \"semimalignant\". A review of the literature concerning leiomyoma and leiomyosarcoma of the central and the peripheral veins showed 91 published cases and no other report of portal vein leiomyoma. The tumors of the vena cava inferior, which have been observed mostly, are listed in particular.", "contents": "[Leiomyoma of the portal vein]. A case report of a 34-year-old female with leiomyoma of the portal vein is presented. The tumor was found accidentally, when laparatomy with diagnosis of cholelithiasis was performed. Total excision of the leiomyoma including a part of the anterior wall of the portal vein was necessary and could be carried out. The continuity of the portal vein could be preserved using continuous vascular suture. Microscopical examination of the tumor showed an increased proliferation rate in the leiomyoma; therefore the tumor was classified \"semimalignant\". A review of the literature concerning leiomyoma and leiomyosarcoma of the central and the peripheral veins showed 91 published cases and no other report of portal vein leiomyoma. The tumors of the vena cava inferior, which have been observed mostly, are listed in particular.", "PMID": 964083}
{"id": "pubmed23n0032_5802", "title": "[Liver resection under occlusion of hepato-duodenal ligaments. Renunciation of a textbook opinion].", "content": "The belief that the hepatoduodenal ligament should only be clamped for 5-10 min, then the clamp released for a shorter or longer period, can no longer be upheld. The author's clinical investigations have shown that continous clamping up to 20 min can be tolerated by the liver without adverse effect. The authors consider a clamping time of 30-40 min tolerable particularly if the liver is cooled by pouring cold Ringer's solution over it. In one case, splenic capsule rupture was a complication of clamping the hepatoduodenal ligament.", "contents": "[Liver resection under occlusion of hepato-duodenal ligaments. Renunciation of a textbook opinion]. The belief that the hepatoduodenal ligament should only be clamped for 5-10 min, then the clamp released for a shorter or longer period, can no longer be upheld. The author's clinical investigations have shown that continous clamping up to 20 min can be tolerated by the liver without adverse effect. The authors consider a clamping time of 30-40 min tolerable particularly if the liver is cooled by pouring cold Ringer's solution over it. In one case, splenic capsule rupture was a complication of clamping the hepatoduodenal ligament.", "PMID": 964084}
{"id": "pubmed23n0032_5803", "title": "[Unusual indication for 2/3 gastroduodenopancreatectomy in ulcer of the papilla, acute pancreatitis and massive ascites].", "content": "A 32-year-old worker, an alcoholic with a history of duodenal ulcer for 2 years, was admitted because of weight loss, severe pain in the upper abdomen, and ascites. Serum lipase was 1200 mU. X-ray showed a possible postbulbar ulcer. Laparotomy was performed, and 10 1 ascitic fluid were evacuated, the latter caused by a peripancreatic callosity compressing the portal vein. multiple necrotic areas in the pancreas; sequestrum cavity in the head of the pancreas connected with a callous ulcer at the duodenal papilla (histology: no malignancy). Two-thirds gastric resection, subtotal duodenopancreatectomy, extirpation of the gallbladder and common bile duct, splenectomy, gastroenteroanastomosis, and antecolic hepatojejunostomy were performed. After a transitory cerebrovascular incident, the patient was discharged on the 34th postoperative day. The patient has gained 11 kg, and works in the construction business. The pathogene development, frequency of complications, and therapy of postbulbar ulcer are described.", "contents": "[Unusual indication for 2/3 gastroduodenopancreatectomy in ulcer of the papilla, acute pancreatitis and massive ascites]. A 32-year-old worker, an alcoholic with a history of duodenal ulcer for 2 years, was admitted because of weight loss, severe pain in the upper abdomen, and ascites. Serum lipase was 1200 mU. X-ray showed a possible postbulbar ulcer. Laparotomy was performed, and 10 1 ascitic fluid were evacuated, the latter caused by a peripancreatic callosity compressing the portal vein. multiple necrotic areas in the pancreas; sequestrum cavity in the head of the pancreas connected with a callous ulcer at the duodenal papilla (histology: no malignancy). Two-thirds gastric resection, subtotal duodenopancreatectomy, extirpation of the gallbladder and common bile duct, splenectomy, gastroenteroanastomosis, and antecolic hepatojejunostomy were performed. After a transitory cerebrovascular incident, the patient was discharged on the 34th postoperative day. The patient has gained 11 kg, and works in the construction business. The pathogene development, frequency of complications, and therapy of postbulbar ulcer are described.", "PMID": 964085}
{"id": "pubmed23n0032_5804", "title": "[Modification of postoperative intestinal atony using caerulein. Comparative studies in the rat and man].", "content": "The effect of caerulein upon postoperative enteral paralysis was investigated in animals and men. The increase in motility effected by cerulein in rats was proved by the increase in passage demonstrated after administration of radioactive test food (51CrO4Na2 solution). A clinical survey comprising 20 patients in the postoperative period following cholecystectomy, compared to an untreated control group, confirmed our findings.", "contents": "[Modification of postoperative intestinal atony using caerulein. Comparative studies in the rat and man]. The effect of caerulein upon postoperative enteral paralysis was investigated in animals and men. The increase in motility effected by cerulein in rats was proved by the increase in passage demonstrated after administration of radioactive test food (51CrO4Na2 solution). A clinical survey comprising 20 patients in the postoperative period following cholecystectomy, compared to an untreated control group, confirmed our findings.", "PMID": 964086}
{"id": "pubmed23n0032_5805", "title": "[Proximal rupture in the triceps surae a typical, but often unrecognized injury].", "content": "An account is given of 12 high ruptures of the triceps surae, a traumatic lesion of the medial belly of the gastrocnemius, caused by sudden overstreching of the muscle by concomitant ankle dorsiflexion and full knee extension. In all cases the authors found characteristic clinical features. Surgical exploration in 5 of 12 cases verified the ruptures of the musculotendinous junction of the medial gastrocnemius belly. A follow-up study of 2 years showed that the surgical treatment seems to be better, especially in younger and athletic patients.", "contents": "[Proximal rupture in the triceps surae a typical, but often unrecognized injury]. An account is given of 12 high ruptures of the triceps surae, a traumatic lesion of the medial belly of the gastrocnemius, caused by sudden overstreching of the muscle by concomitant ankle dorsiflexion and full knee extension. In all cases the authors found characteristic clinical features. Surgical exploration in 5 of 12 cases verified the ruptures of the musculotendinous junction of the medial gastrocnemius belly. A follow-up study of 2 years showed that the surgical treatment seems to be better, especially in younger and athletic patients.", "PMID": 964087}
{"id": "pubmed23n0032_5806", "title": "[Studies on the antibacterial efficacy of Cu-compounds in combination with acridine orange as additive to a polymethylacrylate].", "content": "Copper acetate of suitable specific surface (Kupferacetat Fargua) and copper acetate plus acridin orange intermixed with Palacos powder have bactericidal efficacy against gram-negative and gram-positive bacterias depending on bacterial species. The Palacos osseous cement releases the intermixed substances over long periods of time. There exists a mutual influence on the bactericidal increase of both substances.", "contents": "[Studies on the antibacterial efficacy of Cu-compounds in combination with acridine orange as additive to a polymethylacrylate]. Copper acetate of suitable specific surface (Kupferacetat Fargua) and copper acetate plus acridin orange intermixed with Palacos powder have bactericidal efficacy against gram-negative and gram-positive bacterias depending on bacterial species. The Palacos osseous cement releases the intermixed substances over long periods of time. There exists a mutual influence on the bactericidal increase of both substances.", "PMID": 964088}
{"id": "pubmed23n0032_5807", "title": "Single copy DNA and structural gene sequence relationships among four sea urchin species.", "content": "Measurements of the divergence of single copy DNA sequences among four sea urichin species are presented. At a standard criterion for reassociation (0.12 M phosphate buffer, 60 degrees C, hydroxyapatite binding) we observe the following extents of reaction and reductions in thermal stability for single copy DNA reassociation between Strongylocentrotus purpuratus tracer and heterologous driver DNA: S. dr\u00f6bachiensis 68% and 2.5 degrees C; S. franciscanus 51% and 3.5 degrees C; Lytechinus pictus 12% and 7.5 degrees C. The implied extents of sequence relatedness are consistent with the phylogenetic relationships of these species. The rate of single copy sequence divergence in the evolutionary lines leading to the Strongylocentrotus species is estimated to be 0.06-0.35% per million years. The rate of divergence of total single copy sequence has been compared to that of structural gene sequences represented in S. purpuratus gastrula polysomal messenger RNA. When closely related species, S. purpuratus and S. franciscanus, are compared, these polysomal sequences are found to diverge at a lower rate than does the total single copy sequence. For two very distantly related species, S. purpuratus and L. pictus, a small fraction of the single copy DNA sequence is probably conserved. These conserved sequences are not enriched in their content of structural gene sequences.", "contents": "Single copy DNA and structural gene sequence relationships among four sea urchin species. Measurements of the divergence of single copy DNA sequences among four sea urichin species are presented. At a standard criterion for reassociation (0.12 M phosphate buffer, 60 degrees C, hydroxyapatite binding) we observe the following extents of reaction and reductions in thermal stability for single copy DNA reassociation between Strongylocentrotus purpuratus tracer and heterologous driver DNA: S. dr\u00f6bachiensis 68% and 2.5 degrees C; S. franciscanus 51% and 3.5 degrees C; Lytechinus pictus 12% and 7.5 degrees C. The implied extents of sequence relatedness are consistent with the phylogenetic relationships of these species. The rate of single copy sequence divergence in the evolutionary lines leading to the Strongylocentrotus species is estimated to be 0.06-0.35% per million years. The rate of divergence of total single copy sequence has been compared to that of structural gene sequences represented in S. purpuratus gastrula polysomal messenger RNA. When closely related species, S. purpuratus and S. franciscanus, are compared, these polysomal sequences are found to diverge at a lower rate than does the total single copy sequence. For two very distantly related species, S. purpuratus and L. pictus, a small fraction of the single copy DNA sequence is probably conserved. These conserved sequences are not enriched in their content of structural gene sequences.", "PMID": 964102}
{"id": "pubmed23n0032_5808", "title": "Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei.", "content": "DNA replication in haploid spermatid nuclei has been induced by hybridization of mouse early spermatids to proliferating HeLa cells. Use of polyethylene glycol rather than inactivated Sendai virus as the cell fusion agent was found to be essential to the production of large numbers of heterokaryons containing spermatid nuclei. DNA replication was detected in the heterokaryons by autoradiography. Density of silver grains over spermatid nucleic closely approximated the grain density over labelled HeLa nuclei in the same heterokaryons. Mouse centromeric heterochromatin appeared to be labelled last during the spermatid DNA synthetic period. On the average, HeLa nuclei in heterokaryons began DNA synthesis before spermatid nuclei. Results indicated, however, that DNA synthesis by HeLa nuclei might not be a prerequisite for spermatid DNA synthesis. These experiments demonstrate induction of DNA synthesis in spermatid nuclei, the first major step toward reactivation and recovery of their haploid genome by cell hybridization.", "contents": "Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei. DNA replication in haploid spermatid nuclei has been induced by hybridization of mouse early spermatids to proliferating HeLa cells. Use of polyethylene glycol rather than inactivated Sendai virus as the cell fusion agent was found to be essential to the production of large numbers of heterokaryons containing spermatid nuclei. DNA replication was detected in the heterokaryons by autoradiography. Density of silver grains over spermatid nucleic closely approximated the grain density over labelled HeLa nuclei in the same heterokaryons. Mouse centromeric heterochromatin appeared to be labelled last during the spermatid DNA synthetic period. On the average, HeLa nuclei in heterokaryons began DNA synthesis before spermatid nuclei. Results indicated, however, that DNA synthesis by HeLa nuclei might not be a prerequisite for spermatid DNA synthesis. These experiments demonstrate induction of DNA synthesis in spermatid nuclei, the first major step toward reactivation and recovery of their haploid genome by cell hybridization.", "PMID": 964103}
{"id": "pubmed23n0032_5809", "title": "Chromosome constitution of in vitro segregated haploid and diploid cells of the mouse.", "content": "The composition in segregated haploid sets of paternal and maternal chromosomes has been studied in order to verify whether their composition is uniparental of mixed, fixed or variable. Primary cultures where prepared using kidneys from hybrids of strains of Mus musculus in which the parental chromosomes are distinguishable; the maternal set consists of 20 teleocentric chromosomes, the paternal set of 9 metacentric chromosomes, derived by Robertsonian fusion and 2 telocentrics. Applying Seabright's banding technique, an analysis of segregated haploid and diploid cells, which have originated spontaneously through polyploidisation-segregation processes was carried out. It was concluded that the haploid sets have a variable composition of paternal and maternal chromosomes.", "contents": "Chromosome constitution of in vitro segregated haploid and diploid cells of the mouse. The composition in segregated haploid sets of paternal and maternal chromosomes has been studied in order to verify whether their composition is uniparental of mixed, fixed or variable. Primary cultures where prepared using kidneys from hybrids of strains of Mus musculus in which the parental chromosomes are distinguishable; the maternal set consists of 20 teleocentric chromosomes, the paternal set of 9 metacentric chromosomes, derived by Robertsonian fusion and 2 telocentrics. Applying Seabright's banding technique, an analysis of segregated haploid and diploid cells, which have originated spontaneously through polyploidisation-segregation processes was carried out. It was concluded that the haploid sets have a variable composition of paternal and maternal chromosomes.", "PMID": 964104}
{"id": "pubmed23n0032_5810", "title": "Analysis of the frequency of sister chromatid exchange in different regions of chromosomes of the kangaroo rat (Dipodomys ordii).", "content": "The frequency of sister chromatid exchanges (SCEs) has been determined for C band and non-C band regions of chromosomes of the kangaroo rat after staining with the fluorescence plus giemsa (FPG) technique. After one complete round of DNA synthesis in the presence of bromodeoxyuridine (BrdU) staining of the C band regions revealed simple or complex asymmetries between chromatids. After two complete rounds of DNA synthesis in the presence of BrdU \"harlequin\" chromosomes were observed. Analysis of the distribution of SCE in chromosomes at their 1st and 2nd mitosis showed that relatively few exchanges occur within C band regions, although the frequency of SCEs is high at the junction between C band and non-C band chromosome regions.", "contents": "Analysis of the frequency of sister chromatid exchange in different regions of chromosomes of the kangaroo rat (Dipodomys ordii). The frequency of sister chromatid exchanges (SCEs) has been determined for C band and non-C band regions of chromosomes of the kangaroo rat after staining with the fluorescence plus giemsa (FPG) technique. After one complete round of DNA synthesis in the presence of bromodeoxyuridine (BrdU) staining of the C band regions revealed simple or complex asymmetries between chromatids. After two complete rounds of DNA synthesis in the presence of BrdU \"harlequin\" chromosomes were observed. Analysis of the distribution of SCE in chromosomes at their 1st and 2nd mitosis showed that relatively few exchanges occur within C band regions, although the frequency of SCEs is high at the junction between C band and non-C band chromosome regions.", "PMID": 964105}
{"id": "pubmed23n0032_5811", "title": "Fistula-in-Ano: perineal fistula of intra-abdominal or intrapelvic origin simulating fistula-in-ano--report of seven cases.", "content": "Fistulas manifesting in the perianal region but originating in the abdomen often have their true origin not recognized for extended periods. Examples of seven such complex cases are presented and discussed. Removal of the abdominal abnormality is mandatory before any cure can be effected; the perianal portion usually heals without any special local treatment.", "contents": "Fistula-in-Ano: perineal fistula of intra-abdominal or intrapelvic origin simulating fistula-in-ano--report of seven cases. Fistulas manifesting in the perianal region but originating in the abdomen often have their true origin not recognized for extended periods. Examples of seven such complex cases are presented and discussed. Removal of the abdominal abnormality is mandatory before any cure can be effected; the perianal portion usually heals without any special local treatment.", "PMID": 964107}
{"id": "pubmed23n0032_5812", "title": "Fistula-in-ano: a ten-year follow-up study of horseshoe-abscess fistula-in-ano.", "content": "A preliminary report in 1965 described a conservative surgical procedure for the management of acute and chronic horseshoe anal fistulas. The operation has been used exclusively at the Ochsner Clinic for this problem since 1963. Forty-one patients were treated from 1963 to 1973. The paper reviews the pathology of acute and chronic horseshoe anal fistulas and describes the surgical procedure for both acute and chronic horseshoe abscess anal fistulas with accompanying illustrations. The excellent results with minimal deformity of the anus and anal canal are attributed to avoidance of severing the superficial external sphincter between its coccygeal origin and the anus. Of the 41 patients treated in the period from 1963 to 1973, healing was good, and there has been no recurrence.", "contents": "Fistula-in-ano: a ten-year follow-up study of horseshoe-abscess fistula-in-ano. A preliminary report in 1965 described a conservative surgical procedure for the management of acute and chronic horseshoe anal fistulas. The operation has been used exclusively at the Ochsner Clinic for this problem since 1963. Forty-one patients were treated from 1963 to 1973. The paper reviews the pathology of acute and chronic horseshoe anal fistulas and describes the surgical procedure for both acute and chronic horseshoe abscess anal fistulas with accompanying illustrations. The excellent results with minimal deformity of the anus and anal canal are attributed to avoidance of severing the superficial external sphincter between its coccygeal origin and the anus. Of the 41 patients treated in the period from 1963 to 1973, healing was good, and there has been no recurrence.", "PMID": 964108}
{"id": "pubmed23n0032_5813", "title": "Volvulus of the sigmoid colon: a ten-year study.", "content": "A ten-year study of volvulus of the sigmoid colon is presented. Although the results of treatment of volvulus of the sigmoid colon are generally improving, the challenge for further improvement remains.", "contents": "Volvulus of the sigmoid colon: a ten-year study. A ten-year study of volvulus of the sigmoid colon is presented. Although the results of treatment of volvulus of the sigmoid colon are generally improving, the challenge for further improvement remains.", "PMID": 964112}
{"id": "pubmed23n0032_5814", "title": "Malignant duodenocolic fistula: report of two cases, each with one or more other synchronous gastointestinal cancers.", "content": "Two patients had duodenocolic fistulas, each following a carcinoma of the colon in the area of the hepatic flexure that had perforated into the duodenum. The first patient was treated by a radical pancreatoduodenectomy with right colectomy; the second by subtotal colectomy with excision of the duodenal wall and suture. Both patients are alive and without evidence of recurrent disease. In addition, the first patient had two other primary carcinomas, in the cecum and in the stomach, and the second patient had another primary in the sigmoid. The definitive procedure had to be adjusted to encompass all lesions. The radical operation in one stage seems to be the preferred procedure and certainly is most satisfactory as a cancer operation. Our patient treated by this procedure has survived more than 11 years. An intestinal fistula related to colonic carcinoma, evan though rare, should not be considered as a separate entity. Treatment of the cancer with an en-bloc resection of the communicating organs should be employed if possible.", "contents": "Malignant duodenocolic fistula: report of two cases, each with one or more other synchronous gastointestinal cancers. Two patients had duodenocolic fistulas, each following a carcinoma of the colon in the area of the hepatic flexure that had perforated into the duodenum. The first patient was treated by a radical pancreatoduodenectomy with right colectomy; the second by subtotal colectomy with excision of the duodenal wall and suture. Both patients are alive and without evidence of recurrent disease. In addition, the first patient had two other primary carcinomas, in the cecum and in the stomach, and the second patient had another primary in the sigmoid. The definitive procedure had to be adjusted to encompass all lesions. The radical operation in one stage seems to be the preferred procedure and certainly is most satisfactory as a cancer operation. Our patient treated by this procedure has survived more than 11 years. An intestinal fistula related to colonic carcinoma, evan though rare, should not be considered as a separate entity. Treatment of the cancer with an en-bloc resection of the communicating organs should be employed if possible.", "PMID": 964113}
{"id": "pubmed23n0032_5815", "title": "Adenocarcinoma arising in a sigmoid diverticulum: report of a case.", "content": "A case of adenocarcinoma arising in a sigmoid diverticulum is presented. A patient who had colonic diverticulosis was found to have one diverticular lumen distended by tumor mass. Microscopically, this was seen to be adenocarcinoma arising from the diverticular mucosa. Apparently, this is the first reported case of adenocarcinoma arising in an intact diverticulum of the colon.", "contents": "Adenocarcinoma arising in a sigmoid diverticulum: report of a case. A case of adenocarcinoma arising in a sigmoid diverticulum is presented. A patient who had colonic diverticulosis was found to have one diverticular lumen distended by tumor mass. Microscopically, this was seen to be adenocarcinoma arising from the diverticular mucosa. Apparently, this is the first reported case of adenocarcinoma arising in an intact diverticulum of the colon.", "PMID": 964114}
{"id": "pubmed23n0032_5816", "title": "Gangrene and spontaneous perforation of the cecum as a complication of pseudo-obstruction of the colon: report of three cases and speculation as to etiology.", "content": "\"Pseudo-obstruction of the colon\" refers to a condition in which physical and radiologic findings identical to those associated with mechanical obstruction of the large bowel are found, but in which no organic cause of colonic distention can be identified. These cases may involve progressive proximal large-intestinal dilation to the point of cecal perforation or necrosis. Two cases of spontaneous perforation of the cecum and one case of gangrene of the cecum secondary to proximal distention of the right colon that followed pseudo-obstruction of the colon are presented. Various etiologic factors reported in the medical literature are discussed and analyzed, and an anatomicophysiologic explanation of a possible mechanism, based on sympathetic-parasympathetic neurostimulatory imbalance, is offered.", "contents": "Gangrene and spontaneous perforation of the cecum as a complication of pseudo-obstruction of the colon: report of three cases and speculation as to etiology. \"Pseudo-obstruction of the colon\" refers to a condition in which physical and radiologic findings identical to those associated with mechanical obstruction of the large bowel are found, but in which no organic cause of colonic distention can be identified. These cases may involve progressive proximal large-intestinal dilation to the point of cecal perforation or necrosis. Two cases of spontaneous perforation of the cecum and one case of gangrene of the cecum secondary to proximal distention of the right colon that followed pseudo-obstruction of the colon are presented. Various etiologic factors reported in the medical literature are discussed and analyzed, and an anatomicophysiologic explanation of a possible mechanism, based on sympathetic-parasympathetic neurostimulatory imbalance, is offered.", "PMID": 964115}
{"id": "pubmed23n0032_5817", "title": "[Ultrasonic diagnosis of space-occupying lesions of the kidney (author's transl)].", "content": "In 140 patients who were suspected of having space-occupying lesions of the kidney the ultrasound findings were analysed. There were no false negative results. Out of 4 false positives two could be explained by nontumourous focal renal disease. In a further 10 cases a space-occupying lesion could not be excluded with certainty on the grounds of the ultrasound investigation alone. Out of a total of 96 space-occupying lesions (48 solid tumours, 48 cystic processes) 83 were correctly diagnosed sonographically.", "contents": "[Ultrasonic diagnosis of space-occupying lesions of the kidney (author's transl)]. In 140 patients who were suspected of having space-occupying lesions of the kidney the ultrasound findings were analysed. There were no false negative results. Out of 4 false positives two could be explained by nontumourous focal renal disease. In a further 10 cases a space-occupying lesion could not be excluded with certainty on the grounds of the ultrasound investigation alone. Out of a total of 96 space-occupying lesions (48 solid tumours, 48 cystic processes) 83 were correctly diagnosed sonographically.", "PMID": 964137}
{"id": "pubmed23n0032_5818", "title": "[Dose-activity relationships of the BCG vaccine for newborn infants (author's transl)].", "content": "BCG vaccine with the Kopenhagen strain was injected intracutaneously in 217 newborn infants in four different concentrations (22.000, 50.000, 110.000, and 160.000 organisms per dose). 171 children could be followed up and tested. The results showed a good activity (tuberculin conversion) with a low risk (no ulcers, no severe regional lymph node swelling). There were no complications. As would be expected the conversion rate was highest (95%) with the vaccine with the highest concentration (160.000 U per dose). This concentration is thus recommended for the general BCG vaccination of newborn infants. In contrast to previous experience tuberculin conversion could be demonstrated in 22 babies without a local reaction at the vaccination site. In 13 children the opposite was true.", "contents": "[Dose-activity relationships of the BCG vaccine for newborn infants (author's transl)]. BCG vaccine with the Kopenhagen strain was injected intracutaneously in 217 newborn infants in four different concentrations (22.000, 50.000, 110.000, and 160.000 organisms per dose). 171 children could be followed up and tested. The results showed a good activity (tuberculin conversion) with a low risk (no ulcers, no severe regional lymph node swelling). There were no complications. As would be expected the conversion rate was highest (95%) with the vaccine with the highest concentration (160.000 U per dose). This concentration is thus recommended for the general BCG vaccination of newborn infants. In contrast to previous experience tuberculin conversion could be demonstrated in 22 babies without a local reaction at the vaccination site. In 13 children the opposite was true.", "PMID": 964138}
{"id": "pubmed23n0032_5819", "title": "[Out-patient treatment of periproctitic abscesses (author's transl)].", "content": "A total of 237 patients were treated over a period of five years for anorectal abscesses. Wide circular opening with removal of all purulent foci and necroses from outside had been preferred, because almost only \"late forms\" of anorectal inflammatory processes (perianal and ischiorectal abscesses) were seen. Sphincterotomy was possible in only a few patients. Postoperative fistulae occurred in 14% of the patients.", "contents": "[Out-patient treatment of periproctitic abscesses (author's transl)]. A total of 237 patients were treated over a period of five years for anorectal abscesses. Wide circular opening with removal of all purulent foci and necroses from outside had been preferred, because almost only \"late forms\" of anorectal inflammatory processes (perianal and ischiorectal abscesses) were seen. Sphincterotomy was possible in only a few patients. Postoperative fistulae occurred in 14% of the patients.", "PMID": 964139}
{"id": "pubmed23n0032_5820", "title": "[Elimination of toxic peptides from amanita phalloides by charcoal perfusion in vitro (author's transl)].", "content": "Charcoal perfusion in vitro resulted in virtually complete detoxication of an extract from 170 g of Amanita phalloides in aqueous or plasma protein solution. After 4 min of perfusion the amanitin content was reduced to 1-2% after 15 min it was less than 1% of the starting material. Under the same conditions toadstool toxin was eliminated from plasma protein solution too: after 4 min of perfusion a dose of remaining material, corresponding to the LD70 of the starting material, was nontoxic in mice.", "contents": "[Elimination of toxic peptides from amanita phalloides by charcoal perfusion in vitro (author's transl)]. Charcoal perfusion in vitro resulted in virtually complete detoxication of an extract from 170 g of Amanita phalloides in aqueous or plasma protein solution. After 4 min of perfusion the amanitin content was reduced to 1-2% after 15 min it was less than 1% of the starting material. Under the same conditions toadstool toxin was eliminated from plasma protein solution too: after 4 min of perfusion a dose of remaining material, corresponding to the LD70 of the starting material, was nontoxic in mice.", "PMID": 964140}
{"id": "pubmed23n0032_5821", "title": "[Coarctation of the aorta on the plain chest x-ray (author's transl)].", "content": "Chest X-rays were analysed in 22 male and 16 female patients (mean age 30.7 years) with coarctation of the aorta. The results were correlated with angiography, haemodynamic pressures and operative findings. The left subclavian artery was prominent in 33 cases, signs indicating a collateral circulation (rib notching, internal mammary artery) were present in 26 cases. In addition there were changes of cardiac and aortic configuration. The least reliable sign was constriction of the aorta due to the stenosis itself. Frequent combinations of X-ray signs were dilatation of the left subclavian artery and a collateral circulation, in addition to signs of increased pressure (n = 25). Less frequently (n = 8) only a dilated subclavian artery and signs of increased pressure were found. The prominence of the subclavian artery was particularly marked in systolic pressure gradients through the stenosis of greater than 40 mm Hg, and the formation of a collateral circulation when average aortic pressure differences above and below the stenosis were less than 40 mm Hg. Collaterals were to be found most frequently with a correlation of 2:1 of the systolic gradient to the mean pressure gradient delta Ps/delta Pm. A very good correlation existed comparing X-ray signs with findings at operation. The diagnosis of coarctation of the aorta can thus be made with sufficient certainty from the plain chest X-ray.", "contents": "[Coarctation of the aorta on the plain chest x-ray (author's transl)]. Chest X-rays were analysed in 22 male and 16 female patients (mean age 30.7 years) with coarctation of the aorta. The results were correlated with angiography, haemodynamic pressures and operative findings. The left subclavian artery was prominent in 33 cases, signs indicating a collateral circulation (rib notching, internal mammary artery) were present in 26 cases. In addition there were changes of cardiac and aortic configuration. The least reliable sign was constriction of the aorta due to the stenosis itself. Frequent combinations of X-ray signs were dilatation of the left subclavian artery and a collateral circulation, in addition to signs of increased pressure (n = 25). Less frequently (n = 8) only a dilated subclavian artery and signs of increased pressure were found. The prominence of the subclavian artery was particularly marked in systolic pressure gradients through the stenosis of greater than 40 mm Hg, and the formation of a collateral circulation when average aortic pressure differences above and below the stenosis were less than 40 mm Hg. Collaterals were to be found most frequently with a correlation of 2:1 of the systolic gradient to the mean pressure gradient delta Ps/delta Pm. A very good correlation existed comparing X-ray signs with findings at operation. The diagnosis of coarctation of the aorta can thus be made with sufficient certainty from the plain chest X-ray.", "PMID": 964150}
{"id": "pubmed23n0032_5822", "title": "[ECG changes in dextran intolerance (author's transl)].", "content": "In a 46-year-old patient following infusion of 5-10 ml of dextran ECG changes resembling those of infarction occurred together with severe left-sided chest pain, tachycardia, and an immeasurably low blood pressure. Rapid recovery occurred and normalisation of the ECG within an hour without signs of an intermediate phase. On these grounds it can be assumed that these previously unrecognised changes are probably the expression of a short-term coronary perfusion disturbance as part of an anaphylactoid reaction of the intima.", "contents": "[ECG changes in dextran intolerance (author's transl)]. In a 46-year-old patient following infusion of 5-10 ml of dextran ECG changes resembling those of infarction occurred together with severe left-sided chest pain, tachycardia, and an immeasurably low blood pressure. Rapid recovery occurred and normalisation of the ECG within an hour without signs of an intermediate phase. On these grounds it can be assumed that these previously unrecognised changes are probably the expression of a short-term coronary perfusion disturbance as part of an anaphylactoid reaction of the intima.", "PMID": 964151}
{"id": "pubmed23n0032_5823", "title": "Comparative in vitro biological activity of 1-34 N-terminal synthetic fragments of human parathyroid hormone on bovine and porcine kidney membranes.", "content": "1-34 N-terminal fragments of human parathyroid hormone with sequences according to Brewer et al (hPTHB) and to Niall et al (hPTHN) were synthesized and compared for their ability to activate bovine and porcine kidney cortex membrane adenylate cyclase. Results show that these two hormone sequences are able to activate these membranes but at least in this in vitro assay, hPTHN is about 10 times more active than hPTHB on bovine as well as on porcine membranes. These \"apparent potencies\" with respect to the potency of bPTH 1-34 in bovine and porcine membrane assay systems are respectively 4% and 11% for hPTHB and 39% and 156% for hPTHN. These relative potencies may be interpreted as corresponding to species specificity of the hormone receptor structural relationships.", "contents": "Comparative in vitro biological activity of 1-34 N-terminal synthetic fragments of human parathyroid hormone on bovine and porcine kidney membranes. 1-34 N-terminal fragments of human parathyroid hormone with sequences according to Brewer et al (hPTHB) and to Niall et al (hPTHN) were synthesized and compared for their ability to activate bovine and porcine kidney cortex membrane adenylate cyclase. Results show that these two hormone sequences are able to activate these membranes but at least in this in vitro assay, hPTHN is about 10 times more active than hPTHB on bovine as well as on porcine membranes. These \"apparent potencies\" with respect to the potency of bPTH 1-34 in bovine and porcine membrane assay systems are respectively 4% and 11% for hPTHB and 39% and 156% for hPTHN. These relative potencies may be interpreted as corresponding to species specificity of the hormone receptor structural relationships.", "PMID": 964205}
{"id": "pubmed23n0032_5824", "title": "Induction of uterine ornithine decarboxylase (ODC) by antiestrogens. Inhibition of estradiol-mediated induction of ODC: a possible mechanism of action of antiestrogens.", "content": "The ability of antiestrogens (tamoxifen and nafoxidine) to affect uterine ornithine decarboxylase (ODC) in the ovariectomized rat was determined. Tamoxifen citrate (1 mg or 10 mg/kg) and nafoxidine (0.5 mg/kg) markedly elevated ODC levels. Tamoxifen (1 mg/kg) given for 4 days totally inhibited the E2 (0.5 mug/kg)-mediated induction of ODC. Similarly nafoxidine (0.5 mug/kg) given once a day for 2 days inhibited the E2-mediated induction of ODC. The relation of the inhibition of ODC induction by antiestrogens to their mechanism of action as antiestrogens is discussed.", "contents": "Induction of uterine ornithine decarboxylase (ODC) by antiestrogens. Inhibition of estradiol-mediated induction of ODC: a possible mechanism of action of antiestrogens. The ability of antiestrogens (tamoxifen and nafoxidine) to affect uterine ornithine decarboxylase (ODC) in the ovariectomized rat was determined. Tamoxifen citrate (1 mg or 10 mg/kg) and nafoxidine (0.5 mg/kg) markedly elevated ODC levels. Tamoxifen (1 mg/kg) given for 4 days totally inhibited the E2 (0.5 mug/kg)-mediated induction of ODC. Similarly nafoxidine (0.5 mug/kg) given once a day for 2 days inhibited the E2-mediated induction of ODC. The relation of the inhibition of ODC induction by antiestrogens to their mechanism of action as antiestrogens is discussed.", "PMID": 964206}
{"id": "pubmed23n0032_5825", "title": "Uterine tubulin production during early pregnancy in the rabbit.", "content": "Rabbit uterine tubulin levels rise rapidly in the preimplantation period to peak values of about 10-fold in the endometrium and about 4-5 fold in the myometrium on days 3-5 of pregnancy. Endometrial tubulin falls to non-pregnancy levels by mid-pregnancy while myometrial tubulin declines much more slowly. The rise in tubulin exceeds the early rate of increase in total uterine protein and DNA. Following implantation, tubulin content of the decidual basal plate area increases rapidly to peak about day 12 then declines. Production of uterine tubulin appears capable of a major, short term response to gonadal hormone stimulation.", "contents": "Uterine tubulin production during early pregnancy in the rabbit. Rabbit uterine tubulin levels rise rapidly in the preimplantation period to peak values of about 10-fold in the endometrium and about 4-5 fold in the myometrium on days 3-5 of pregnancy. Endometrial tubulin falls to non-pregnancy levels by mid-pregnancy while myometrial tubulin declines much more slowly. The rise in tubulin exceeds the early rate of increase in total uterine protein and DNA. Following implantation, tubulin content of the decidual basal plate area increases rapidly to peak about day 12 then declines. Production of uterine tubulin appears capable of a major, short term response to gonadal hormone stimulation.", "PMID": 964207}
{"id": "pubmed23n0032_5826", "title": "The half-life of luteinizing hormone in intact and castrated quail during photoperiodic stimulation.", "content": "In the Japanese quail (Coturnix coturnix japonica) the plasma concentration of luteinizing hormone (LH) depends on the physiological state of the animal. Low levels are found in sexually immature birds on short days (0.8 +/- 0.1 (n = 20) ng/ml) while after exposure to long days, sexually mature birds have a significantly higher LH level (4.4 +/- 0.4 (n = 20) ng/ml). Castration of long day males results in an even higher rise in LH concentration to 16.8 +/- 1.8 (n = 16) ng/ml. When the half-life (t1/2) of circulating LH was determined it was found to have two components: a fast t 1/2 of about 6 min and a slower component of either 20 min (determined by a radioisotope method), or 40 min (following hypophysectomy). No significant difference was observed in either of these components between birds in the three physiological states (short days, long days, castration plus long days). It would appear, therefore, that since the half-life of plasma LH remains constant, the difference in circulating LH observed under the various physiological conditions results solely from a change in the rate of LH secretion.", "contents": "The half-life of luteinizing hormone in intact and castrated quail during photoperiodic stimulation. In the Japanese quail (Coturnix coturnix japonica) the plasma concentration of luteinizing hormone (LH) depends on the physiological state of the animal. Low levels are found in sexually immature birds on short days (0.8 +/- 0.1 (n = 20) ng/ml) while after exposure to long days, sexually mature birds have a significantly higher LH level (4.4 +/- 0.4 (n = 20) ng/ml). Castration of long day males results in an even higher rise in LH concentration to 16.8 +/- 1.8 (n = 16) ng/ml. When the half-life (t1/2) of circulating LH was determined it was found to have two components: a fast t 1/2 of about 6 min and a slower component of either 20 min (determined by a radioisotope method), or 40 min (following hypophysectomy). No significant difference was observed in either of these components between birds in the three physiological states (short days, long days, castration plus long days). It would appear, therefore, that since the half-life of plasma LH remains constant, the difference in circulating LH observed under the various physiological conditions results solely from a change in the rate of LH secretion.", "PMID": 964208}
{"id": "pubmed23n0032_5827", "title": "Continuous measurement and rapid kinetics of ATP synthesis in rat liver mitochondria, mitoplasts and inner membrane vesicles determined by firefly-luciferase luminescence.", "content": "Oxidative phosphorylation in rat liver mitochondria, the mitoplast or inner membrane-matrix fraction, and inverted inner membrane vesicles was studied with firefly luciferase luminescence. All preparations showed relatively high ATP/O ratios under initial reaction conditions in the presence of Mg2+, but only intact mitochondria demonstrated significant respiratory control. Mitochondria and mitoplasts, but not vesicles, catalyzed nearly complete conversion of ADP to ATP. Vesicles converted between 50% and 70% of added ADP to ATP establishing a phosphate potential of 9.8 to 10.5 kcal/mol (43.9 kJ/mol). In \"loosely coupled\" submitochondrial systems which do not display respiratory control, the phosphate potential appears to be a useful parameter of the integrity of the phosphorylating membrane, while ATP/O ratios may be dependent on the relative concentrations of ADP and ATP in the reaction medium. The rapid kinetics of oxidative synthesis of ATP by inverted inner membrane vesicles was investigated in stopped-flow rapid-mixing experiments. After an oxygen pulse to reduced vesicles, ATP synthesis commenced within 20 ms. In antimycin-inhibited vesicles, ATP synthesis resulting from the rapid oxidation of the terminal portion of the respiratory chain commenced within 20ms and took approximately 100 ms for half completion. Since half oxidation of the terminal portion of the respiratory chain occurs within a few milliseconds, ATP synthesis resulting from such redox reactions must occur over a significantly longer time course than the redox reactions themselves.", "contents": "Continuous measurement and rapid kinetics of ATP synthesis in rat liver mitochondria, mitoplasts and inner membrane vesicles determined by firefly-luciferase luminescence. Oxidative phosphorylation in rat liver mitochondria, the mitoplast or inner membrane-matrix fraction, and inverted inner membrane vesicles was studied with firefly luciferase luminescence. All preparations showed relatively high ATP/O ratios under initial reaction conditions in the presence of Mg2+, but only intact mitochondria demonstrated significant respiratory control. Mitochondria and mitoplasts, but not vesicles, catalyzed nearly complete conversion of ADP to ATP. Vesicles converted between 50% and 70% of added ADP to ATP establishing a phosphate potential of 9.8 to 10.5 kcal/mol (43.9 kJ/mol). In \"loosely coupled\" submitochondrial systems which do not display respiratory control, the phosphate potential appears to be a useful parameter of the integrity of the phosphorylating membrane, while ATP/O ratios may be dependent on the relative concentrations of ADP and ATP in the reaction medium. The rapid kinetics of oxidative synthesis of ATP by inverted inner membrane vesicles was investigated in stopped-flow rapid-mixing experiments. After an oxygen pulse to reduced vesicles, ATP synthesis commenced within 20 ms. In antimycin-inhibited vesicles, ATP synthesis resulting from the rapid oxidation of the terminal portion of the respiratory chain commenced within 20ms and took approximately 100 ms for half completion. Since half oxidation of the terminal portion of the respiratory chain occurs within a few milliseconds, ATP synthesis resulting from such redox reactions must occur over a significantly longer time course than the redox reactions themselves.", "PMID": 964235}
{"id": "pubmed23n0032_5828", "title": "The structure of pentasaccharides and hexasaccharides from blood group substance H.", "content": "Six individual reduced pentasaccharides and hexasaccharides were isolated after sodium borohydride degradation of blood group substance H from pig stomach linings. Their structure was determined by means of periodate oxidation, methylation analysis, mass spectrometry, partial acid and enzymic hydrolysis and chromic anhydride oxidation. It has been found that all the oligosaccharides investigated contain the common tetrasaccharide core to which terminal alpha-L-fucose and N-acetyl-alpha-D-glucosamine residues are attached. Some problems of carbohydrate chain heterogeneity in blood group substances are discussed.", "contents": "The structure of pentasaccharides and hexasaccharides from blood group substance H. Six individual reduced pentasaccharides and hexasaccharides were isolated after sodium borohydride degradation of blood group substance H from pig stomach linings. Their structure was determined by means of periodate oxidation, methylation analysis, mass spectrometry, partial acid and enzymic hydrolysis and chromic anhydride oxidation. It has been found that all the oligosaccharides investigated contain the common tetrasaccharide core to which terminal alpha-L-fucose and N-acetyl-alpha-D-glucosamine residues are attached. Some problems of carbohydrate chain heterogeneity in blood group substances are discussed.", "PMID": 964236}
{"id": "pubmed23n0032_5829", "title": "Human-polymorphonuclear-leucocyte neutral protease and its inhibitor. Studies with fluorescein-labelled polymeric collagen fibrils as a substrate.", "content": "Human polymorphonuclear leucocytes were obtained from the synovial fluids of patients with inflamed knee joints suffering either from Reiter's syndrome or from rheumatoid arthritis. The polymorphonuclear leucocytes were collected by gentle centrifugation followed by disruption and their subcellular fractionation by centrifugation in 0.34 M sucrose to provide a granule fraction and a post-granule supernatant fraction. 0.5 M KCl extraction of the granule fraction yielded neutral protease activity, similar to trypsin, when assayed against fluorescein-labelled polymeric collagen fibrils. The post-granule supernatant fraction contained an inhibitor towards the neutral protease and trypsin. The inhibition of the neutral protease was found to be time-dependent, this inhibition being released after 1.5-2 h. In contrast, the inhibition of trypsin was irreversible and this property was used to devise an assay procedure for the inhibitor.", "contents": "Human-polymorphonuclear-leucocyte neutral protease and its inhibitor. Studies with fluorescein-labelled polymeric collagen fibrils as a substrate. Human polymorphonuclear leucocytes were obtained from the synovial fluids of patients with inflamed knee joints suffering either from Reiter's syndrome or from rheumatoid arthritis. The polymorphonuclear leucocytes were collected by gentle centrifugation followed by disruption and their subcellular fractionation by centrifugation in 0.34 M sucrose to provide a granule fraction and a post-granule supernatant fraction. 0.5 M KCl extraction of the granule fraction yielded neutral protease activity, similar to trypsin, when assayed against fluorescein-labelled polymeric collagen fibrils. The post-granule supernatant fraction contained an inhibitor towards the neutral protease and trypsin. The inhibition of the neutral protease was found to be time-dependent, this inhibition being released after 1.5-2 h. In contrast, the inhibition of trypsin was irreversible and this property was used to devise an assay procedure for the inhibitor.", "PMID": 964237}
{"id": "pubmed23n0032_5830", "title": "Synthesis of the brain-specific S-100 protein in a cell-free system from wheat embryo programmed with poly(A)-containing RNA from rabbit brain.", "content": "Polyadenylated polysomal RNA was prepared from rabbit cerebral hemispheres using phenol extraction and chromatography on oligo(dT)-cellulose. This RNA directed the synthesis of the brain-specific S-100 protein in cell-free extracts from wheat embryo. S-100 protein was absent from the products of endogenous incorporation and from a reaction programmed with kidney mRNA. These results suggest that S-100 protein mRNA contains a poly(adenylic acid) sequence and rule out the necessity of a brain-specific factor for translation of -S100 protein mRNA.", "contents": "Synthesis of the brain-specific S-100 protein in a cell-free system from wheat embryo programmed with poly(A)-containing RNA from rabbit brain. Polyadenylated polysomal RNA was prepared from rabbit cerebral hemispheres using phenol extraction and chromatography on oligo(dT)-cellulose. This RNA directed the synthesis of the brain-specific S-100 protein in cell-free extracts from wheat embryo. S-100 protein was absent from the products of endogenous incorporation and from a reaction programmed with kidney mRNA. These results suggest that S-100 protein mRNA contains a poly(adenylic acid) sequence and rule out the necessity of a brain-specific factor for translation of -S100 protein mRNA.", "PMID": 964238}
{"id": "pubmed23n0032_5831", "title": "Structural determination of three glycoasparagines isolated from the urine of a patient with aspartylglycosaminuria.", "content": "Three different glycoasparagines have been isolated from the urine of a patient with aspartylglycosaminuria and their structures determined using sugar, amino acid and methylation analysis, enzymic degradation and measurements of the optical rotations. The structures were 2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 135 mg/l) beta-D-galactopyranosyl-(1 leads to 4)-2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 35 mg/l), and alpha-D-mannopyranosyl-(1 leads to 6)-beta-D-mannopyranosyl-(1 leads to 4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1 leads to 4)-2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 30 mg/l). The first two compounds have previously been described, whereas the third compound is different from any of the glycoasparagines isolated before.", "contents": "Structural determination of three glycoasparagines isolated from the urine of a patient with aspartylglycosaminuria. Three different glycoasparagines have been isolated from the urine of a patient with aspartylglycosaminuria and their structures determined using sugar, amino acid and methylation analysis, enzymic degradation and measurements of the optical rotations. The structures were 2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 135 mg/l) beta-D-galactopyranosyl-(1 leads to 4)-2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 35 mg/l), and alpha-D-mannopyranosyl-(1 leads to 6)-beta-D-mannopyranosyl-(1 leads to 4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1 leads to 4)-2-acetamido-1-N-(4'-L-aspartyl)-2-deoxy-beta-D-glucopyranosylamine (yield 30 mg/l). The first two compounds have previously been described, whereas the third compound is different from any of the glycoasparagines isolated before.", "PMID": 964239}
{"id": "pubmed23n0032_5832", "title": "Structure and function of rat-liver ribosomes. Modification by 2-methoxy-5-nitrotropone treatment.", "content": "Rat liver ribosomes and 60-S ribosome subunits were treated with the primary-amino-group-specific reagent 2-methoxy-5-nitrotropone. Important differences in the sensitivity of several ribosomal activities to inactivation by the reagent were observed. While elongation-factor-dependent activities are totally abolished in the treated particles, peptidyl transferase activity is either unaffected in 60-S ribosomal subunits or even strongly stimulated in 80-S ribosomes. Analysis of the ribosomal proteins modified by nitrotropone made it possible to draw some conclusions on their accessibility in the ribosomal structure and to relate some proteins with their involvement in the ribosome active centers. Thus, proteins L3, L13, L15 and L23 seem to be in a rather well protected position while proteins L10, L35, L37, X1 and X2 are totally exposed to the reagent. The protein accessibility also depends on the ribosome conformation, proteins L14 and L17, for instance, being sensitive in 80-S ribosomes and protected in 60-S subunits. In relation to the implication of proteins in functional centers, the data presented here together with other data obtained from protein-deficient core particles seem to indicate a possible role of proteins L21 and/or L26 in the peptidyl transferase center.", "contents": "Structure and function of rat-liver ribosomes. Modification by 2-methoxy-5-nitrotropone treatment. Rat liver ribosomes and 60-S ribosome subunits were treated with the primary-amino-group-specific reagent 2-methoxy-5-nitrotropone. Important differences in the sensitivity of several ribosomal activities to inactivation by the reagent were observed. While elongation-factor-dependent activities are totally abolished in the treated particles, peptidyl transferase activity is either unaffected in 60-S ribosomal subunits or even strongly stimulated in 80-S ribosomes. Analysis of the ribosomal proteins modified by nitrotropone made it possible to draw some conclusions on their accessibility in the ribosomal structure and to relate some proteins with their involvement in the ribosome active centers. Thus, proteins L3, L13, L15 and L23 seem to be in a rather well protected position while proteins L10, L35, L37, X1 and X2 are totally exposed to the reagent. The protein accessibility also depends on the ribosome conformation, proteins L14 and L17, for instance, being sensitive in 80-S ribosomes and protected in 60-S subunits. In relation to the implication of proteins in functional centers, the data presented here together with other data obtained from protein-deficient core particles seem to indicate a possible role of proteins L21 and/or L26 in the peptidyl transferase center.", "PMID": 964240}
{"id": "pubmed23n0032_5833", "title": "A novel pathway for the synthesis of solanidine in the isolated chloroplast from greening potatoes.", "content": "Synthesis of chlorophyll always preceded the synthesis of solanine in cold-stored potatoes in the presence of dim light (30--40 lux). Isolated chloroplasts from green peelings were able to fix CO2. The evidence obtained showed a direct reduction of CO2 to formate, which was the primary product of fixation. Apart from this the chloroplasts were capable of incorporating NaH14CO3, [14C]formate, [2-14/C]glycine, [2-14C]pyruvate, [2-14C]acetate, [2-14C]mevalonate and [U-14C]serine into the solanidine moiety of the alkaloid. The intermediates in the pathway between CO2 and acetate have been identified as formate, glycine, serine and pyruvate. The localization of the enzymes involved in the reaction sequence viz. serine hydroxymethyl-transferase, and pyruvic dehydrogenase complex, in the isolated chloroplast has been established.", "contents": "A novel pathway for the synthesis of solanidine in the isolated chloroplast from greening potatoes. Synthesis of chlorophyll always preceded the synthesis of solanine in cold-stored potatoes in the presence of dim light (30--40 lux). Isolated chloroplasts from green peelings were able to fix CO2. The evidence obtained showed a direct reduction of CO2 to formate, which was the primary product of fixation. Apart from this the chloroplasts were capable of incorporating NaH14CO3, [14C]formate, [2-14/C]glycine, [2-14C]pyruvate, [2-14C]acetate, [2-14C]mevalonate and [U-14C]serine into the solanidine moiety of the alkaloid. The intermediates in the pathway between CO2 and acetate have been identified as formate, glycine, serine and pyruvate. The localization of the enzymes involved in the reaction sequence viz. serine hydroxymethyl-transferase, and pyruvic dehydrogenase complex, in the isolated chloroplast has been established.", "PMID": 964241}
{"id": "pubmed23n0032_5834", "title": "The keratin chains of avian scale tissue. Sequence heterogeneity and the number of scale keratin genes.", "content": "The three major proteins of chick scale keratin were isolated as their S-carboxymethylated derivatives and shown to be similar or identical in molecular weight by gel filtration but to be distinct by amino acid analysis and gel electrophoresis. The major amino-terminal sequence of scale keratin chains was determined and shown to be highly homologous to the corresponding region of feather keratin chains. The carboxyl-terminal peptides of the three scale keratin fractions differed in sequence but were all homologous to the carboxyl-terminal segment of feather keratin chains. The pronounced concentration of cysteine residues at the amino-terminal and carboxyl-terminal segments suggested a similar role for these regions in both scale and feather keratin chains, namely to provide a disulphide-linked matrix to maintain the organisation of fibrils which arise from the internal hydrophobic segments of both types of chain. Analysis of a large hydrophobic segment from each of the three isolated protein fractions revealed that each was composed largely of repeating tripeptide units of the type Gly-Gly-X (where X = Phe, Leu or Tyr). At a few positions in each hydrophobic peptide, microheterogeneity was apparent in the sequences indicating that each isolated protein fraction was composed of at least three different chains each encoded by a different gene. A minimum of nine keratin genes are therefore expressed in scale tissue.", "contents": "The keratin chains of avian scale tissue. Sequence heterogeneity and the number of scale keratin genes. The three major proteins of chick scale keratin were isolated as their S-carboxymethylated derivatives and shown to be similar or identical in molecular weight by gel filtration but to be distinct by amino acid analysis and gel electrophoresis. The major amino-terminal sequence of scale keratin chains was determined and shown to be highly homologous to the corresponding region of feather keratin chains. The carboxyl-terminal peptides of the three scale keratin fractions differed in sequence but were all homologous to the carboxyl-terminal segment of feather keratin chains. The pronounced concentration of cysteine residues at the amino-terminal and carboxyl-terminal segments suggested a similar role for these regions in both scale and feather keratin chains, namely to provide a disulphide-linked matrix to maintain the organisation of fibrils which arise from the internal hydrophobic segments of both types of chain. Analysis of a large hydrophobic segment from each of the three isolated protein fractions revealed that each was composed largely of repeating tripeptide units of the type Gly-Gly-X (where X = Phe, Leu or Tyr). At a few positions in each hydrophobic peptide, microheterogeneity was apparent in the sequences indicating that each isolated protein fraction was composed of at least three different chains each encoded by a different gene. A minimum of nine keratin genes are therefore expressed in scale tissue.", "PMID": 964242}
{"id": "pubmed23n0032_5835", "title": "Pyridoxal 5'-phosphate and the regulation of ornithine decarboxylase activity and stability.", "content": "There are two forms of ornithine decarboxylase with respect to pyridoxal 5'-phosphate (pyridoxal-P) affinity in exponentially-growing Swiss 3T3 mouse fibroblasts: form I (Km approximately 10 muM) accounts for 30% of the total activity, and form II (Km approximately 0.4 muM) the remainder. Each form of the enzyme is in rapid equilibrium with ornithine and pyridoxal-P; neither form recognizes the Schiff base between ornithine and pyridoxal-P as a substrate. Total pyridoxal-P concentrations indicate that both forms may normally be at least partially active in vivo. Upon stimulation of 3T3 cells by pituitary growth factors, form I becomes undetectable within 4 h. As total activity increases over 10-fold during this time and continues to increase thereafter, a possible conversion of form I to form II could account for this increase only if the Km change reflects other changes in preexisting enzyme. The rates of cofactor dissociation are apparently the same for each form and neither rate changes with the growth state. Since rapid equilibrium kinetics apply, the forms apparently differ in their rate of cofactor association. The half-lives of the two forms in vivo are the same in unstimulated cells when measured concurrently. Also, the half-life of total activity decreases markedly upon stimulation as form II becomes dominant. These and other observations are not consistent with pyridoxal-P serving a major protective function for the enzyme in vivo.", "contents": "Pyridoxal 5'-phosphate and the regulation of ornithine decarboxylase activity and stability. There are two forms of ornithine decarboxylase with respect to pyridoxal 5'-phosphate (pyridoxal-P) affinity in exponentially-growing Swiss 3T3 mouse fibroblasts: form I (Km approximately 10 muM) accounts for 30% of the total activity, and form II (Km approximately 0.4 muM) the remainder. Each form of the enzyme is in rapid equilibrium with ornithine and pyridoxal-P; neither form recognizes the Schiff base between ornithine and pyridoxal-P as a substrate. Total pyridoxal-P concentrations indicate that both forms may normally be at least partially active in vivo. Upon stimulation of 3T3 cells by pituitary growth factors, form I becomes undetectable within 4 h. As total activity increases over 10-fold during this time and continues to increase thereafter, a possible conversion of form I to form II could account for this increase only if the Km change reflects other changes in preexisting enzyme. The rates of cofactor dissociation are apparently the same for each form and neither rate changes with the growth state. Since rapid equilibrium kinetics apply, the forms apparently differ in their rate of cofactor association. The half-lives of the two forms in vivo are the same in unstimulated cells when measured concurrently. Also, the half-life of total activity decreases markedly upon stimulation as form II becomes dominant. These and other observations are not consistent with pyridoxal-P serving a major protective function for the enzyme in vivo.", "PMID": 964243}
{"id": "pubmed23n0032_5836", "title": "A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures.", "content": "A comparison was made of some kinetic properties of three chicken lactate dehydrogenase isoenzymes (1, 3 and 5) at 4, 16, 23 and 40 degrees C. Assays were performed with an enzyme concentration of 0.01 muM at pH 6.0. Under the conditions of assay, lactate dehydrogenase 3 and 5 bound to the particulate fraction of homogenized skeletal muscle and were evaluated in the soluble and particulate state. Binding of isoenzymes 3 and5 to the cellular particulate fraction decreased V. This decrease was much greater for lactate dehydrogenase 5 and 3. Values of V for lactate dehydrogenase isoenzymes 1 and 3 did not follow a simple Arrhenius relationship; there was a rapid change in activity between 16 and 23 degrees C. The apparent Km (pyruvate) values of all isoenzymes (bound or soluble) increased with increasing temperature, changing 4--10-fold. The apparent Km for lactate dehydrogenase 5 was greater than that for lactate dehydrogenase 3, which in turn was greater than that for lactate dehydrogenase 1.", "contents": "A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures. A comparison was made of some kinetic properties of three chicken lactate dehydrogenase isoenzymes (1, 3 and 5) at 4, 16, 23 and 40 degrees C. Assays were performed with an enzyme concentration of 0.01 muM at pH 6.0. Under the conditions of assay, lactate dehydrogenase 3 and 5 bound to the particulate fraction of homogenized skeletal muscle and were evaluated in the soluble and particulate state. Binding of isoenzymes 3 and5 to the cellular particulate fraction decreased V. This decrease was much greater for lactate dehydrogenase 5 and 3. Values of V for lactate dehydrogenase isoenzymes 1 and 3 did not follow a simple Arrhenius relationship; there was a rapid change in activity between 16 and 23 degrees C. The apparent Km (pyruvate) values of all isoenzymes (bound or soluble) increased with increasing temperature, changing 4--10-fold. The apparent Km for lactate dehydrogenase 5 was greater than that for lactate dehydrogenase 3, which in turn was greater than that for lactate dehydrogenase 1.", "PMID": 964244}
{"id": "pubmed23n0032_5837", "title": "Structure-activity relationships in acetylcholinesterase reactions. Hydrolysis of non-ionic acetic esters.", "content": "The Michaelis-Menten parameters kcat, Ks(app) and the second-order rate constants kII = k2/Ks of acetylcholinesterase-catalyzed hydrolysis of 25 acetic esters with non-ionic leaving groups have been determined at 25 degree C and pH 7.5 in 0.15 M KCL. A linear relationship between the substrate noncovalent binding capacity and the leaving group hydrophobicity, and a multiparameter correlation of the acetylation reaction rate constant logarithm with the leaving group inductive effect, hydrophobicity, and steric effect, have been established. The acetyl-enzyme deacetylation rate constant has been calculated. Taken together, a fairly complete understanding of acetylcholinesterase specificity is possible. The data are consistent with a model of the acetylcholinesterase active site, in which the catalytically active groups are located at the bottom of a jaws-like slit with a limited range of hydrophobic walls that provide the sorption of the substrate leaving groups not longer than that in n-butyl acetate.", "contents": "Structure-activity relationships in acetylcholinesterase reactions. Hydrolysis of non-ionic acetic esters. The Michaelis-Menten parameters kcat, Ks(app) and the second-order rate constants kII = k2/Ks of acetylcholinesterase-catalyzed hydrolysis of 25 acetic esters with non-ionic leaving groups have been determined at 25 degree C and pH 7.5 in 0.15 M KCL. A linear relationship between the substrate noncovalent binding capacity and the leaving group hydrophobicity, and a multiparameter correlation of the acetylation reaction rate constant logarithm with the leaving group inductive effect, hydrophobicity, and steric effect, have been established. The acetyl-enzyme deacetylation rate constant has been calculated. Taken together, a fairly complete understanding of acetylcholinesterase specificity is possible. The data are consistent with a model of the acetylcholinesterase active site, in which the catalytically active groups are located at the bottom of a jaws-like slit with a limited range of hydrophobic walls that provide the sorption of the substrate leaving groups not longer than that in n-butyl acetate.", "PMID": 964245}
{"id": "pubmed23n0032_5838", "title": "Synthesis of a class of DNA-binding proteins in synchronized untransformed and virus-transformed cells.", "content": "The synthesis of a classs of proteins with affinity for denatured DNA has been studied in synchronized cultures of the established hamster fibroblast line HIL-1 and its virus-transformed derivative NIL-1-hamster sarcoma virus. It has been found that the synthesis of a DNA-binding protein (P6, molecular weight 50 000) in synchronized untransformed NIL-1 cells follows a pattern different from that observed in the transformed cells. The protein is low in stationary cultures of NIL-1 and NIL-1-hamster sarcoma virus but increases after the cells are stimulated to grow, although the time of maximal P6 synthesis relative to cellular DNA synthesis is different in NIL-1 and NIL-1-hamster sarcoma virus. In contrast, the pattern of synthesis of two other DNA-proteins (P7 and P8') in essentially identical in synchronized untransformed and transformed cells. P7 is very low in resting cultures of both types of cells, but greatly increases early after the cells are stimulated to divide, while P8'is hish in stationary cultures and decreases slightly after the initiation of cellular DNA synthesis has occurred.", "contents": "Synthesis of a class of DNA-binding proteins in synchronized untransformed and virus-transformed cells. The synthesis of a classs of proteins with affinity for denatured DNA has been studied in synchronized cultures of the established hamster fibroblast line HIL-1 and its virus-transformed derivative NIL-1-hamster sarcoma virus. It has been found that the synthesis of a DNA-binding protein (P6, molecular weight 50 000) in synchronized untransformed NIL-1 cells follows a pattern different from that observed in the transformed cells. The protein is low in stationary cultures of NIL-1 and NIL-1-hamster sarcoma virus but increases after the cells are stimulated to grow, although the time of maximal P6 synthesis relative to cellular DNA synthesis is different in NIL-1 and NIL-1-hamster sarcoma virus. In contrast, the pattern of synthesis of two other DNA-proteins (P7 and P8') in essentially identical in synchronized untransformed and transformed cells. P7 is very low in resting cultures of both types of cells, but greatly increases early after the cells are stimulated to divide, while P8'is hish in stationary cultures and decreases slightly after the initiation of cellular DNA synthesis has occurred.", "PMID": 964246}
{"id": "pubmed23n0032_5839", "title": "Non-equivalence of human hemoglobin chains in the oxidation-reduction and heme-transfer reactions. A 13C nuclear-Magnetic-resonance study.", "content": "12C nuclear magnetic resonance (NMR) spectroscopy has been applied to the investigation of chain non-equivalence for two reactions of human hemoglobin: oxidation-reduction and hemetransfer. The method is based on previous observations that in the carbonyl region, Hb13CO gives two well-resolved resonances which arise from 13C of carbonyls bound respectively to the alpha and beta chains; moreover, integration of spectra allows on e to estimate their relative abundance. A mixture of ferrous and ferric hemoglobins in dye-mediated oxidation-reduction equilibrium can be formally considered to be equivalent to two redox couples in equilibrium, namely alphaIII/alphaII and betaIII/betaII; from a knowledge of these ratios, one can conclude whether the chains are equivalent or not in their oxidation-reduction properties. In this work, these ratios were evaluated by reacting the redox systems with 13CO and integrating the 13C NMR spectra. The results show differences in the intrinsic oxidation-reduction potentials of the chains in hemoglobin tetramer, E1/2(beta)being higher than E1/2(alpha)in neutral solution but not at pH9 and above. The binding of inositol hexakisphosphate does not modify the difference between beta and alpha though substantially increasing the overall potential The results are discussed in the light of current hypotheses to account for the change of Hill coefficient with pH for the reaction studied. The non-equivalence of chains is shown also for heme transfer from methemoglobin. For the phosphate-free protein, the beta chains lose heme more rapidly than that alpha chains; the addition of inositol hexakisphosphate results in the decrease of overall heme transfer as well as of chain heterogeneity.", "contents": "Non-equivalence of human hemoglobin chains in the oxidation-reduction and heme-transfer reactions. A 13C nuclear-Magnetic-resonance study. 12C nuclear magnetic resonance (NMR) spectroscopy has been applied to the investigation of chain non-equivalence for two reactions of human hemoglobin: oxidation-reduction and hemetransfer. The method is based on previous observations that in the carbonyl region, Hb13CO gives two well-resolved resonances which arise from 13C of carbonyls bound respectively to the alpha and beta chains; moreover, integration of spectra allows on e to estimate their relative abundance. A mixture of ferrous and ferric hemoglobins in dye-mediated oxidation-reduction equilibrium can be formally considered to be equivalent to two redox couples in equilibrium, namely alphaIII/alphaII and betaIII/betaII; from a knowledge of these ratios, one can conclude whether the chains are equivalent or not in their oxidation-reduction properties. In this work, these ratios were evaluated by reacting the redox systems with 13CO and integrating the 13C NMR spectra. The results show differences in the intrinsic oxidation-reduction potentials of the chains in hemoglobin tetramer, E1/2(beta)being higher than E1/2(alpha)in neutral solution but not at pH9 and above. The binding of inositol hexakisphosphate does not modify the difference between beta and alpha though substantially increasing the overall potential The results are discussed in the light of current hypotheses to account for the change of Hill coefficient with pH for the reaction studied. The non-equivalence of chains is shown also for heme transfer from methemoglobin. For the phosphate-free protein, the beta chains lose heme more rapidly than that alpha chains; the addition of inositol hexakisphosphate results in the decrease of overall heme transfer as well as of chain heterogeneity.", "PMID": 964247}
{"id": "pubmed23n0032_5840", "title": "Structural studies of chicken erythrocyte histone H5.", "content": "Spectroscopic studies (nuclear magnetic resonance, circular dichroism and infrared) have been carried out on chicken erythrocyte histone H5 and on  three peptides cleaved therefrom: 1-31, 32-197 and 58-197. It is shown that at ionic strengths above o.1M part of the H5 molecule takes up a globular conformation containing 14% alpha helix but no beta sheet structure. Several details of the circular dichroism and nuclear magnetic resonace spectra indicate that the globular region is located in the N-terminal half of the molecule and this proposal is supported by the observation that the peptide 32-197 is largely incapable of folding and the peptide 59-197 is completely incapable of folding. Structural similarities and differences between histone H5 and histone H1 are discussed.", "contents": "Structural studies of chicken erythrocyte histone H5. Spectroscopic studies (nuclear magnetic resonance, circular dichroism and infrared) have been carried out on chicken erythrocyte histone H5 and on  three peptides cleaved therefrom: 1-31, 32-197 and 58-197. It is shown that at ionic strengths above o.1M part of the H5 molecule takes up a globular conformation containing 14% alpha helix but no beta sheet structure. Several details of the circular dichroism and nuclear magnetic resonace spectra indicate that the globular region is located in the N-terminal half of the molecule and this proposal is supported by the observation that the peptide 32-197 is largely incapable of folding and the peptide 59-197 is completely incapable of folding. Structural similarities and differences between histone H5 and histone H1 are discussed.", "PMID": 964248}
{"id": "pubmed23n0032_5841", "title": "Calcium regulation in heart cells. The interaction of mitochondrial and sarcoplasmic reticulum with troponin-bound calcium.", "content": "A study has been carried out of the interaction of dog heart mitochondria and sarcoplasmic reticulum with Ca2+ bound to troponin or to its Ca2+-binding component (troponin-C). Both organelles are able to release more than 80% of the Ca2+ bound to the two proteins. However, sarcoplasmic reticulum is only able to do so in the presence of oxalate, whereas mitochondria are active also in the absence of permeant Ca-complexing anions. On the other hand, calcium uptake in heart mitochondria is severely inhibited by Mg2+. The results are discussed with reference to the problem of the control of Ca2+ in heart sarcoplasm.", "contents": "Calcium regulation in heart cells. The interaction of mitochondrial and sarcoplasmic reticulum with troponin-bound calcium. A study has been carried out of the interaction of dog heart mitochondria and sarcoplasmic reticulum with Ca2+ bound to troponin or to its Ca2+-binding component (troponin-C). Both organelles are able to release more than 80% of the Ca2+ bound to the two proteins. However, sarcoplasmic reticulum is only able to do so in the presence of oxalate, whereas mitochondria are active also in the absence of permeant Ca-complexing anions. On the other hand, calcium uptake in heart mitochondria is severely inhibited by Mg2+. The results are discussed with reference to the problem of the control of Ca2+ in heart sarcoplasm.", "PMID": 964249}
{"id": "pubmed23n0032_5842", "title": "The structure of Artemia salina haemoglobins. A comparative characterisation of four naupliar and adult heamoglobins.", "content": "Haemoglobins of Artemia salina were investigated during development and the results obtained are summarised as follows. a)A new haemoglobin (haemoglobin N) was induced de novo in the hatching nauplius, but not in the prenauplius nor in the embryo. b)Haemoglobin N seemed not to be synthesised in the adult. c)The adult contained three distinct haemoglobins (haemoglobins I, II and III) in a quantitative ratio of 1:6:3, respectively, and these haemoglobins were not detected in nauplius. d)Isoelectric points determined were 5.7,5.6,5.7, and 5.9 for haemoglobin N, I, II and III, respectively. e)All four haemoglobins contained one large polypeptide (Mr= 1.1-1.3 X 10(5)) and possibly two minir polypeptides (Mr = 8-9.5 X 10(4) and Mr = 5.4 X qo(4), respectively).", "contents": "The structure of Artemia salina haemoglobins. A comparative characterisation of four naupliar and adult heamoglobins. Haemoglobins of Artemia salina were investigated during development and the results obtained are summarised as follows. a)A new haemoglobin (haemoglobin N) was induced de novo in the hatching nauplius, but not in the prenauplius nor in the embryo. b)Haemoglobin N seemed not to be synthesised in the adult. c)The adult contained three distinct haemoglobins (haemoglobins I, II and III) in a quantitative ratio of 1:6:3, respectively, and these haemoglobins were not detected in nauplius. d)Isoelectric points determined were 5.7,5.6,5.7, and 5.9 for haemoglobin N, I, II and III, respectively. e)All four haemoglobins contained one large polypeptide (Mr= 1.1-1.3 X 10(5)) and possibly two minir polypeptides (Mr = 8-9.5 X 10(4) and Mr = 5.4 X qo(4), respectively).", "PMID": 964250}
{"id": "pubmed23n0032_5843", "title": "Acetyl-CoA hydrolase; activity, regulation and physiological significance of the enzyme in brown adipose tissue from hamster.", "content": "1. Acetate production in hamster brown adipose tissue is a consequence of the existence of an acetyl-CoA hydrolase. The enzyme is soluble and is localised to the mitochondrial matrix. 2. Acetyl-CoA hydrolase has an apparent Km for acetyl-CoA of 51 muM and a specific activity at 30 degrees C of 870 nmol of acetate formed/min per mg 100 000 X g supernatant protein. 3. The enzyme is noncompetitively activated by ADP and inhibited by NADH and the effect of these nucleotides may well serve to regulate the enzyme activity in vivo. 4. A strong product inhibition by CoA is observed. The inhibition is of S-linear-I-hyperbolic noncompetitive nature. 5. The hydrolase has a q10 of 2.0, which represents a 7.3% change in the rat of acetate production per degrees C. The energy of activation is 12,200 cal/mol (53905 J/mol). 6. The regulatory role of acetyl-CoA hydrolase for fatty acid oxidation in brown adipose tissue of the hamster (a hibernator) at low as well as at normal body temperature is discussed.", "contents": "Acetyl-CoA hydrolase; activity, regulation and physiological significance of the enzyme in brown adipose tissue from hamster. 1. Acetate production in hamster brown adipose tissue is a consequence of the existence of an acetyl-CoA hydrolase. The enzyme is soluble and is localised to the mitochondrial matrix. 2. Acetyl-CoA hydrolase has an apparent Km for acetyl-CoA of 51 muM and a specific activity at 30 degrees C of 870 nmol of acetate formed/min per mg 100 000 X g supernatant protein. 3. The enzyme is noncompetitively activated by ADP and inhibited by NADH and the effect of these nucleotides may well serve to regulate the enzyme activity in vivo. 4. A strong product inhibition by CoA is observed. The inhibition is of S-linear-I-hyperbolic noncompetitive nature. 5. The hydrolase has a q10 of 2.0, which represents a 7.3% change in the rat of acetate production per degrees C. The energy of activation is 12,200 cal/mol (53905 J/mol). 6. The regulatory role of acetyl-CoA hydrolase for fatty acid oxidation in brown adipose tissue of the hamster (a hibernator) at low as well as at normal body temperature is discussed.", "PMID": 964251}
{"id": "pubmed23n0032_5844", "title": "Solubilization of the chromatin-bound estrogen receptor from chicken liver and fractionation on hydroxylapatite.", "content": "1. High-affinity estrogen-binding sites can be solubilized from the liver chromatin of estrogenized chickens by treatment of the chromatin with 2 M KCL/5 M urea and fractionation on hydroxylapatite. Two estrogen-binding proteins are eluted from hydroxylapatite columns by 20mM phosphate (binding protein I) and 200mMphosphate (binding protein II), respectively. 2. The binding protein I is part of a non-histone protein fraction containing acid-soluble and insoluble proteins, whereas the binding protein II elutes together with high molecular weight nonhistone proteins containing acid insoluble proteins only. Both binding proteins exhibit the smae affinity for estradiol (Kd approximately 10(-9) M). 3. From chromatin of untreated chickens very small amounts of binding protein I (0.1 pmol/mg protein compared to 1.9 pmol/mg protein from estrogenized chickens) with the smae affinity for estradiol as that from estrogenized animals can be solubilized. Binding protein II is not detectable. 4. The \"soluble nuclear estrogen receptor\" extracted from crude liver nucleir of estrogenized chickens by 0.5 M KCL behaves on hydroxylapatite very similarly to salt/urea-dissociated chromatin with respect to the binding protein I. No binding protein II, however, can be demonstrated. 5. Chromatography of various preparations on Bio-Gel A-1.5 m indicates that the binding protein II is a residual chromatin fragment containing an unseparated binding protein-DNA complex, whereas the binding protein I represents the solubilized nucleic-acid-free chromosomal estrogen receptor. The \"soluble nuclear receptor\" and the binding protein I, however, are not identical with respect to their chromatographic behaviour on Bio-Gel A-1.5m, even though their estrogen binding entity remaining after trypsin treatment seems to be very similar.", "contents": "Solubilization of the chromatin-bound estrogen receptor from chicken liver and fractionation on hydroxylapatite. 1. High-affinity estrogen-binding sites can be solubilized from the liver chromatin of estrogenized chickens by treatment of the chromatin with 2 M KCL/5 M urea and fractionation on hydroxylapatite. Two estrogen-binding proteins are eluted from hydroxylapatite columns by 20mM phosphate (binding protein I) and 200mMphosphate (binding protein II), respectively. 2. The binding protein I is part of a non-histone protein fraction containing acid-soluble and insoluble proteins, whereas the binding protein II elutes together with high molecular weight nonhistone proteins containing acid insoluble proteins only. Both binding proteins exhibit the smae affinity for estradiol (Kd approximately 10(-9) M). 3. From chromatin of untreated chickens very small amounts of binding protein I (0.1 pmol/mg protein compared to 1.9 pmol/mg protein from estrogenized chickens) with the smae affinity for estradiol as that from estrogenized animals can be solubilized. Binding protein II is not detectable. 4. The \"soluble nuclear estrogen receptor\" extracted from crude liver nucleir of estrogenized chickens by 0.5 M KCL behaves on hydroxylapatite very similarly to salt/urea-dissociated chromatin with respect to the binding protein I. No binding protein II, however, can be demonstrated. 5. Chromatography of various preparations on Bio-Gel A-1.5 m indicates that the binding protein II is a residual chromatin fragment containing an unseparated binding protein-DNA complex, whereas the binding protein I represents the solubilized nucleic-acid-free chromosomal estrogen receptor. The \"soluble nuclear receptor\" and the binding protein I, however, are not identical with respect to their chromatographic behaviour on Bio-Gel A-1.5m, even though their estrogen binding entity remaining after trypsin treatment seems to be very similar.", "PMID": 964252}
{"id": "pubmed23n0032_5845", "title": "Functional identity of the monomeric and multiple forms of elongation-factor 1 from Krebs-II mouse ascites-tumor cells.", "content": "Highly purified 3H-labelled elongation factor 1 (EF-1) from Krebs II mouse ascites tumour cells was separated into biologically active monomeric and aggregate forms of the enzyme by either gradient centrifugation or gel filtration. When corrected for their content of inactive enzyme both forms of the factor were found to be equally active whether tested in the binding or synthesis reaction. The only form of the enzyme found bound to ribosomes was the monomer; it was therefore concluded that the aggregate form of the enzyme must first dissociate before it reacts with the ribosome. The stoichiometry of the aminoacyl-tRNA binding reaction to ribosomes in the presence of guanosine nucleotides was also studied. It was found that one molecule of aminoacyl-tRNA and of Guo-5'-P2-CH2-P is bound per molecule of EF-1 bound to the ribosome. Following interaction with a release from, the ribosomes, EF-1 was found to be predominantly monomeric.", "contents": "Functional identity of the monomeric and multiple forms of elongation-factor 1 from Krebs-II mouse ascites-tumor cells. Highly purified 3H-labelled elongation factor 1 (EF-1) from Krebs II mouse ascites tumour cells was separated into biologically active monomeric and aggregate forms of the enzyme by either gradient centrifugation or gel filtration. When corrected for their content of inactive enzyme both forms of the factor were found to be equally active whether tested in the binding or synthesis reaction. The only form of the enzyme found bound to ribosomes was the monomer; it was therefore concluded that the aggregate form of the enzyme must first dissociate before it reacts with the ribosome. The stoichiometry of the aminoacyl-tRNA binding reaction to ribosomes in the presence of guanosine nucleotides was also studied. It was found that one molecule of aminoacyl-tRNA and of Guo-5'-P2-CH2-P is bound per molecule of EF-1 bound to the ribosome. Following interaction with a release from, the ribosomes, EF-1 was found to be predominantly monomeric.", "PMID": 964253}
{"id": "pubmed23n0032_5846", "title": "The kinetics in vivo of the synthesis of albumin-like protein and albumin in rats.", "content": "The incorporation of radioactivity into total protein, albumin-like protein and albumin in liver and into total protein and albumin in blood plasma, was studied for a time period of 2 h after intraportal injection of L-[1-14C]leucine. 1. In the liver, radioactivity began to increase in albumin-like protein before 2.5 min and in albumin at about 10 min after injection. In the plasma, radioactive albumin appeared at about 15 min. No albumin-like protein could be detected in the plasma. 2. Maximum radioactivity was reached first in albumin-like protein, then in hepatic albumin and finally in albumin in the blood-stream. The maximum specific radioactivity of albumin-like protein was 15 times higher than that of extravascular hepatic albumin which, in turn, was 6 times higher than that of plasma albumin. 3. The increase of radioactivity in albumin in the blood corresponded almost quantitatively to the decrease of radioactivity in albumin-like protein. 4. It is concluded that the albumin-like protein is the precursor of albumin in vivo. It is converted into albumin 5-6 min before the appearance of newly synthesized albumin in the blood-stream.", "contents": "The kinetics in vivo of the synthesis of albumin-like protein and albumin in rats. The incorporation of radioactivity into total protein, albumin-like protein and albumin in liver and into total protein and albumin in blood plasma, was studied for a time period of 2 h after intraportal injection of L-[1-14C]leucine. 1. In the liver, radioactivity began to increase in albumin-like protein before 2.5 min and in albumin at about 10 min after injection. In the plasma, radioactive albumin appeared at about 15 min. No albumin-like protein could be detected in the plasma. 2. Maximum radioactivity was reached first in albumin-like protein, then in hepatic albumin and finally in albumin in the blood-stream. The maximum specific radioactivity of albumin-like protein was 15 times higher than that of extravascular hepatic albumin which, in turn, was 6 times higher than that of plasma albumin. 3. The increase of radioactivity in albumin in the blood corresponded almost quantitatively to the decrease of radioactivity in albumin-like protein. 4. It is concluded that the albumin-like protein is the precursor of albumin in vivo. It is converted into albumin 5-6 min before the appearance of newly synthesized albumin in the blood-stream.", "PMID": 964254}
{"id": "pubmed23n0032_5847", "title": "The amino-acid sequence of the alpha-crystallin A chains of red kangaroo and Virginia opossum.", "content": "The amino acid sequence of the A chain of the eye lens protein alpha-crystallin from the red kangaroo (Macropus rufus) was completely determined by manual Edman degradation of tryptic, thermolytic and cyanogen bromide peptides. The sequence of the alpha-crystallin A chain from the Virginia opossum (Didelphis marsupialis) was deduced from amino acid analyses and partial Edman degradation of peptides. The 173-residue A chains of kangaroo and opossum differ in six positions, whereas comparison with the bovine alpha-crystallin A chain reveals 17 and 22 substitutions, respectively. Most substitutions occur in the COOH-terminal part of the chain.", "contents": "The amino-acid sequence of the alpha-crystallin A chains of red kangaroo and Virginia opossum. The amino acid sequence of the A chain of the eye lens protein alpha-crystallin from the red kangaroo (Macropus rufus) was completely determined by manual Edman degradation of tryptic, thermolytic and cyanogen bromide peptides. The sequence of the alpha-crystallin A chain from the Virginia opossum (Didelphis marsupialis) was deduced from amino acid analyses and partial Edman degradation of peptides. The 173-residue A chains of kangaroo and opossum differ in six positions, whereas comparison with the bovine alpha-crystallin A chain reveals 17 and 22 substitutions, respectively. Most substitutions occur in the COOH-terminal part of the chain.", "PMID": 964255}
{"id": "pubmed23n0032_5848", "title": "Hamster brown-adipose-tissue mitochondria. The role of fatty acids in the control of the proton conductance of the inner membrane.", "content": "The specific ability of fatty acids to increase the proton conductance of the inner membrane of mitochondria from the liver and brown adipose tissue of cold-adapted hamsters was compared. The liver and brown-adipose-tissue mitochondria had their effective proton conductances increased by respectively 0.028 and 0.94 nmol H+- min-1. (mV of proton electrochemical gradient)-1 for each nmol of palmitate bound. No difference could be detected between the abilities of liver and brown-adipose-tissue mitochondria to bind fatty acids. Purine nucleotides did not displace farry acids from the brown-adipase-tissue mitochondria. The endogenous fatty acid content of hamster brown-adipose-tissue mitochondria prepared in the absence of album was found to be equivalent to 17 +/-  7 nmol of palmitate/mg protein. The fatty acid content was reduced to 1 nmol/mg after preincubation of the mitochondria with CoA, ATP and carnitine. No inert pool of fatty acids could be detected. The endogenous fatty acids of hamster liver mitochondria were less than 4 nmol of palmitate equivalent/mg protein. Some of the fatty acid associated with the brown-adipose-tissue mitochondria originates during preparation of the mitochondria. In the light of these results, the physiological role of the fatty acids in controlling the proton conductance of the brown-adipose-tissue mitochondrial inner membrane, and hence- non-shivering thermogenesis, is re-evaluated.", "contents": "Hamster brown-adipose-tissue mitochondria. The role of fatty acids in the control of the proton conductance of the inner membrane. The specific ability of fatty acids to increase the proton conductance of the inner membrane of mitochondria from the liver and brown adipose tissue of cold-adapted hamsters was compared. The liver and brown-adipose-tissue mitochondria had their effective proton conductances increased by respectively 0.028 and 0.94 nmol H+- min-1. (mV of proton electrochemical gradient)-1 for each nmol of palmitate bound. No difference could be detected between the abilities of liver and brown-adipose-tissue mitochondria to bind fatty acids. Purine nucleotides did not displace farry acids from the brown-adipase-tissue mitochondria. The endogenous fatty acid content of hamster brown-adipose-tissue mitochondria prepared in the absence of album was found to be equivalent to 17 +/-  7 nmol of palmitate/mg protein. The fatty acid content was reduced to 1 nmol/mg after preincubation of the mitochondria with CoA, ATP and carnitine. No inert pool of fatty acids could be detected. The endogenous fatty acids of hamster liver mitochondria were less than 4 nmol of palmitate equivalent/mg protein. Some of the fatty acid associated with the brown-adipose-tissue mitochondria originates during preparation of the mitochondria. In the light of these results, the physiological role of the fatty acids in controlling the proton conductance of the brown-adipose-tissue mitochondrial inner membrane, and hence- non-shivering thermogenesis, is re-evaluated.", "PMID": 964256}
{"id": "pubmed23n0032_5849", "title": "Messenger RNA translation in the presence of homologous and heterologous tRNA.", "content": "The effect of tRNA distribution in the cell on the rate of specific protein synthesis has been investigated. A tRNA-dependent cell-free protein-synthesizing system derived from Krebs-II ascites cells has been worked out. In this system it is possible to synthesize specific proteins (egg-white proteins or globin) by the translation of oviduct or reticulocyte messenger RNA in the presence of tRNA from homologous or heterologous tissues. The rate of translation of a give messenger RNA is optimal in the presence of tRNA from the homologous tissue. The lower level of protein synthesis obtained with an excess of heterologous tRNA can be overcome by adding the homologous tRNA. Nevertheless homologous isoaccepting species partly purified by a reversed-phase chromatography or a benzoylated DEAE-cellulose column cannot fully restore the optimal synthesis, eliminating the hypothesis according to which a particular tRNA species is involved in this phenomenon. The correct distribution of tRNA is necessary for optimal translation of a messenger RNA.", "contents": "Messenger RNA translation in the presence of homologous and heterologous tRNA. The effect of tRNA distribution in the cell on the rate of specific protein synthesis has been investigated. A tRNA-dependent cell-free protein-synthesizing system derived from Krebs-II ascites cells has been worked out. In this system it is possible to synthesize specific proteins (egg-white proteins or globin) by the translation of oviduct or reticulocyte messenger RNA in the presence of tRNA from homologous or heterologous tissues. The rate of translation of a give messenger RNA is optimal in the presence of tRNA from the homologous tissue. The lower level of protein synthesis obtained with an excess of heterologous tRNA can be overcome by adding the homologous tRNA. Nevertheless homologous isoaccepting species partly purified by a reversed-phase chromatography or a benzoylated DEAE-cellulose column cannot fully restore the optimal synthesis, eliminating the hypothesis according to which a particular tRNA species is involved in this phenomenon. The correct distribution of tRNA is necessary for optimal translation of a messenger RNA.", "PMID": 964257}
{"id": "pubmed23n0032_5850", "title": "Isolation and chracterization of rat-lens messenger RNAs. Comparison of lens proteins, synthesized in lens culture and in homologous and heterologous cell-free systems.", "content": "Messenger RNA has been isolated from rat lens tissue. The mRNA species which codes for the A2 chain of alpha-crystallin, revealed the same extremely high sedimentation value (14S) as the corresponding messenger from calf lens. However, it has been shown that in rat lens the 14-S messenger preparation directs the synthesis of an additional alpha-crystallin chain, designated as alpha-X with an approximate molecular weight of 24000. For comparative purpose crystallin synthesis has also been studied as well in cultured rat lens cells as in the rat lens cell-free system.", "contents": "Isolation and chracterization of rat-lens messenger RNAs. Comparison of lens proteins, synthesized in lens culture and in homologous and heterologous cell-free systems. Messenger RNA has been isolated from rat lens tissue. The mRNA species which codes for the A2 chain of alpha-crystallin, revealed the same extremely high sedimentation value (14S) as the corresponding messenger from calf lens. However, it has been shown that in rat lens the 14-S messenger preparation directs the synthesis of an additional alpha-crystallin chain, designated as alpha-X with an approximate molecular weight of 24000. For comparative purpose crystallin synthesis has also been studied as well in cultured rat lens cells as in the rat lens cell-free system.", "PMID": 964258}
{"id": "pubmed23n0032_5851", "title": "Identification of a membrane protein involved in mitochondrial phosphate transport.", "content": "A specific labeling by radioactive N-ethylmaleimide of a protein involved in phosphate transport was obtained by protecting one of the two-SH-groups of the transport system with low concentrations of mersalyl. Subsequently, the other free-SH groups were blocked with excess N-ethylmaleimide. Removal of mersalyl by cysteine and subsequent inbucation with labeled N-ethylmaleimide results in a \"specific\" binding of N-ethylmaleimide to one-SH group functionally involved in phosphate transport. The isolated inner membrane fraction of the labeled mitochondria was subjected to dodecylsulfate gel electrophoresis. The followin results were obtained. 1. The difference of the radioactivity pattern on the dodecylsulfate-polyacrylamide gel of inner membrane proteins, labeled with N-[14C]ethylmaleimide in the absence and with N-[3H-A1-ethylmaleimide in the presence of mersalyl during preincubation of mitochondria, shows only one main labeled peak. The same labeled peak is obtained from the difference of labeling after preincubation with a constant low concentration of mersalyl at 32 degrees C and at 0 degrees C. 2. The position of the labeled peak on the dodecylsulfate-polyacrylamide gel corresponds to a protein of molecular weight of 26500 +/- 800. 3. The amount of one of the two-SH groups, involved in phosphate transport, was estimated to be 30 nmol per g of mitochondrial protein.", "contents": "Identification of a membrane protein involved in mitochondrial phosphate transport. A specific labeling by radioactive N-ethylmaleimide of a protein involved in phosphate transport was obtained by protecting one of the two-SH-groups of the transport system with low concentrations of mersalyl. Subsequently, the other free-SH groups were blocked with excess N-ethylmaleimide. Removal of mersalyl by cysteine and subsequent inbucation with labeled N-ethylmaleimide results in a \"specific\" binding of N-ethylmaleimide to one-SH group functionally involved in phosphate transport. The isolated inner membrane fraction of the labeled mitochondria was subjected to dodecylsulfate gel electrophoresis. The followin results were obtained. 1. The difference of the radioactivity pattern on the dodecylsulfate-polyacrylamide gel of inner membrane proteins, labeled with N-[14C]ethylmaleimide in the absence and with N-[3H-A1-ethylmaleimide in the presence of mersalyl during preincubation of mitochondria, shows only one main labeled peak. The same labeled peak is obtained from the difference of labeling after preincubation with a constant low concentration of mersalyl at 32 degrees C and at 0 degrees C. 2. The position of the labeled peak on the dodecylsulfate-polyacrylamide gel corresponds to a protein of molecular weight of 26500 +/- 800. 3. The amount of one of the two-SH groups, involved in phosphate transport, was estimated to be 30 nmol per g of mitochondrial protein.", "PMID": 964259}
{"id": "pubmed23n0032_5852", "title": "Light-induced increase in the activity of maize plastid DNA-dependent RNA polymerase.", "content": "Illumination of dark-grown maize plants induces a rapid rise in DNA-DEPENDENT RNA polymerase activity in plastids. Within 16 h illumination the enzyme activity is isolated plastids increases 3-4 fold as compared to other dark-grown plants illuminated for only 2 h. This change in activity is unaccompanied by either a quantitative rise in the amount of chloroplast RNA polymerase or qualitative changes in the purified enzyme. It is suggested that other factors may be present which interact either with the enzyme or the DNA template and that these factors are responsible for the light-induced enhancement of the RNA polymerase activity inplastids.", "contents": "Light-induced increase in the activity of maize plastid DNA-dependent RNA polymerase. Illumination of dark-grown maize plants induces a rapid rise in DNA-DEPENDENT RNA polymerase activity in plastids. Within 16 h illumination the enzyme activity is isolated plastids increases 3-4 fold as compared to other dark-grown plants illuminated for only 2 h. This change in activity is unaccompanied by either a quantitative rise in the amount of chloroplast RNA polymerase or qualitative changes in the purified enzyme. It is suggested that other factors may be present which interact either with the enzyme or the DNA template and that these factors are responsible for the light-induced enhancement of the RNA polymerase activity inplastids.", "PMID": 964260}
{"id": "pubmed23n0032_5853", "title": "Isopycnic centrifugation of thyroid iodoproteins: selectivity of endocytosis.", "content": "Isopycnic centrifugation in RbCl was shown to be an effective method both for evaluating the iodine content of thyroglobulin labelled in vivo and for the fractionation of thyroglobulin molecules as a function of their iodine content. Iodination and degradation of thyroid iodoproteins were studied by this method and by zonal centrifugation in sucrose density gradients. Based on these methods it was shown that iodination in vivo is a selective process, 19-S and poorly iodinated thyroglobulin having a higher reactivity toward iodine than 27-S and iodine-rich thyroglobulin. The disappearance of iodoproteins from the thyroid was evaluated by equilibrium, labelling the iodoproteins, blocking iodine incorporation with thiourea derivatives and observing (by sucrose gradient and by RbCl isopycnic centrifugation) at different times the properties of the remaining molecules. Among molecules of different size (19-S and 27-S) and among molecules of the same size (19 S) but different iodine content it was shown that reabsorption from the thyroid gland occurred at the same rate. It was concluded, therefore, that the degradative pathway is essentially a random, non-selective process. Newly iodinated (pulse-labelled) iodoproteins were degraded faster than preexisting molecules. Among the pool of those newly iodinated thyroid proteins, 27-S molecules were reabsorbed faster than 19-S molecules and iodine-rich thyroglobulin molecules were reabsorbed faster than the iodine-poor ones. Since iodination in vivo occurs as repeated pulses of iodine incorporation, it is suggested that this latter phenomenon is a regulatory mechanism which minimizes degradation of molecules which are iodine-poor and have a lower hormonal content.", "contents": "Isopycnic centrifugation of thyroid iodoproteins: selectivity of endocytosis. Isopycnic centrifugation in RbCl was shown to be an effective method both for evaluating the iodine content of thyroglobulin labelled in vivo and for the fractionation of thyroglobulin molecules as a function of their iodine content. Iodination and degradation of thyroid iodoproteins were studied by this method and by zonal centrifugation in sucrose density gradients. Based on these methods it was shown that iodination in vivo is a selective process, 19-S and poorly iodinated thyroglobulin having a higher reactivity toward iodine than 27-S and iodine-rich thyroglobulin. The disappearance of iodoproteins from the thyroid was evaluated by equilibrium, labelling the iodoproteins, blocking iodine incorporation with thiourea derivatives and observing (by sucrose gradient and by RbCl isopycnic centrifugation) at different times the properties of the remaining molecules. Among molecules of different size (19-S and 27-S) and among molecules of the same size (19 S) but different iodine content it was shown that reabsorption from the thyroid gland occurred at the same rate. It was concluded, therefore, that the degradative pathway is essentially a random, non-selective process. Newly iodinated (pulse-labelled) iodoproteins were degraded faster than preexisting molecules. Among the pool of those newly iodinated thyroid proteins, 27-S molecules were reabsorbed faster than 19-S molecules and iodine-rich thyroglobulin molecules were reabsorbed faster than the iodine-poor ones. Since iodination in vivo occurs as repeated pulses of iodine incorporation, it is suggested that this latter phenomenon is a regulatory mechanism which minimizes degradation of molecules which are iodine-poor and have a lower hormonal content.", "PMID": 964261}
{"id": "pubmed23n0032_5854", "title": "Structure and synthesis of a lipid-containing bacteriophage. Purification, chemical composition, and partial sequences of the structural proteins.", "content": "The four structural proteins of the lipid-containing bacteriophage PM2 have been purified by dissociation of the virus in the presence of acetic acid followed by a combination of gel filtration and ion-exchange chromatography in the presence of sodium dodecylsulfate and guanidine hydrochloride. Amino acid analyses of each of the proteins were performed and correlated with the properties and functions of the proteins. Protein I has the highest polarity and is the only water-soluble protein. Protein II has a rather high polarity and hydrophobicity index and probably interacts electrostatically and hydrophobically with the bilayer. Proteins III and IV have low polarities and possess the solubility properties of proteolipids. At least protein III and perhaps also protein IV may interact with the bilayer. No fatty acids are covalently linked to these proteins. Tryptic fingerprints showed that proteins I and II contain a high proportion of hydrophobic peptides, but especially protein I also contains a large number of hydrophilic peptides. Proteins III and IV have relatively few hydrophobic peptides despite their relatively high hydrophobicity. Protein IV has two distinct regions, as shown by partial sequence studies. Basic amino acids at the N-terminus would serve for interaction with the viral DNA, the following hydrophobic sequence might interact with protein III or with the bilayer.", "contents": "Structure and synthesis of a lipid-containing bacteriophage. Purification, chemical composition, and partial sequences of the structural proteins. The four structural proteins of the lipid-containing bacteriophage PM2 have been purified by dissociation of the virus in the presence of acetic acid followed by a combination of gel filtration and ion-exchange chromatography in the presence of sodium dodecylsulfate and guanidine hydrochloride. Amino acid analyses of each of the proteins were performed and correlated with the properties and functions of the proteins. Protein I has the highest polarity and is the only water-soluble protein. Protein II has a rather high polarity and hydrophobicity index and probably interacts electrostatically and hydrophobically with the bilayer. Proteins III and IV have low polarities and possess the solubility properties of proteolipids. At least protein III and perhaps also protein IV may interact with the bilayer. No fatty acids are covalently linked to these proteins. Tryptic fingerprints showed that proteins I and II contain a high proportion of hydrophobic peptides, but especially protein I also contains a large number of hydrophilic peptides. Proteins III and IV have relatively few hydrophobic peptides despite their relatively high hydrophobicity. Protein IV has two distinct regions, as shown by partial sequence studies. Basic amino acids at the N-terminus would serve for interaction with the viral DNA, the following hydrophobic sequence might interact with protein III or with the bilayer.", "PMID": 964262}
{"id": "pubmed23n0032_5855", "title": "Matrix-bound phosphoglucose isomerase. Formation and properties of monomers and hybrids.", "content": "Dimeric phosphoglucose isomerase from rabbit muscle was immobilized by reaction with cyanogen-bromide-activated Sephrose 4B. The catalytic parameters and stability properties of the free and matrix-bound isomerases were essentially identical. Total monomerisation of the matrix-bound enzyme was achieved with 8 M urea as determined by a study in which one subunit was labelled with iodo[14C]acetate and the other with the 3H-labelled reagent. Although matrix-bound monomers were devoid of isomerase activity, they were still capable of binding the substrate. Matrix-bound monomers exhibited the ability to redimerize with soluble isomerase subunits from either rabbit or human yielding catalytically active dimers. Yeast isomerase monomers, in contrast, did not yield active hybrids with the rabbit monomers. Furthermore, soluble subunits, which had been inactivated with pyridoxal 5'-phosphate were also capable of hybridizing with and inducing catalytic activity in the matrix-bound monomers. These studies indicate the prerequisite of dimer formation for catalytic activity but the independent action of the catalytic centers of the dimer.", "contents": "Matrix-bound phosphoglucose isomerase. Formation and properties of monomers and hybrids. Dimeric phosphoglucose isomerase from rabbit muscle was immobilized by reaction with cyanogen-bromide-activated Sephrose 4B. The catalytic parameters and stability properties of the free and matrix-bound isomerases were essentially identical. Total monomerisation of the matrix-bound enzyme was achieved with 8 M urea as determined by a study in which one subunit was labelled with iodo[14C]acetate and the other with the 3H-labelled reagent. Although matrix-bound monomers were devoid of isomerase activity, they were still capable of binding the substrate. Matrix-bound monomers exhibited the ability to redimerize with soluble isomerase subunits from either rabbit or human yielding catalytically active dimers. Yeast isomerase monomers, in contrast, did not yield active hybrids with the rabbit monomers. Furthermore, soluble subunits, which had been inactivated with pyridoxal 5'-phosphate were also capable of hybridizing with and inducing catalytic activity in the matrix-bound monomers. These studies indicate the prerequisite of dimer formation for catalytic activity but the independent action of the catalytic centers of the dimer.", "PMID": 964263}
{"id": "pubmed23n0032_5856", "title": "Effects of prolactin and progesterone on expression of casein genes. Titration of casein mRNA by hybridization with complementary DNA.", "content": "Prolactin injected into pseudopregnant rabbits induced lactogenesis. Progesterone injected simultaneously considerably reduced this effect. The content of mRNA for casein in total cellular RNA and in total polysomal RNA was estimated during these two hormonal actions using a hybridization technique. The synthesis of DNA complementary to casein mRNA was carried out with mRNA obtained from polysomes immunoprecipitated with anti-casein antibody. The content of casein mRNA was determined by hybridization of this cDNA with the RNA fractions. This technique revealed that prolactin induced the synthesis of mRNA for casein whereas progresterone greatly limited this effect of prolactin. These variations of casein mRNA content are in good agreement with values previously determined by translation and with the ability of mammary gland to synthesize casein.", "contents": "Effects of prolactin and progesterone on expression of casein genes. Titration of casein mRNA by hybridization with complementary DNA. Prolactin injected into pseudopregnant rabbits induced lactogenesis. Progesterone injected simultaneously considerably reduced this effect. The content of mRNA for casein in total cellular RNA and in total polysomal RNA was estimated during these two hormonal actions using a hybridization technique. The synthesis of DNA complementary to casein mRNA was carried out with mRNA obtained from polysomes immunoprecipitated with anti-casein antibody. The content of casein mRNA was determined by hybridization of this cDNA with the RNA fractions. This technique revealed that prolactin induced the synthesis of mRNA for casein whereas progresterone greatly limited this effect of prolactin. These variations of casein mRNA content are in good agreement with values previously determined by translation and with the ability of mammary gland to synthesize casein.", "PMID": 964265}
{"id": "pubmed23n0032_5857", "title": "Formation of extracellular protein A by Staphylococcus aureus.", "content": "Staphylococcus aureus contains cell wall protein A as well as extracellular protein A. The two types of protein A have very similar amino acid compositions, electrophoretic mobilities and sizes. The release of extracellular protein A from exponentially growing bacteria is dependent on protein synthesis de novo and protein A is released directly after being synthesized on the ribosomes. Bacteria in the stationary phase, however, release protein A as a result of cell lysis. Protoplasts have been isolated which produce protein A as extensively as the intact bacteria but because of the absence of of the formation of extracellular protein A is observed from cells also producing cell wall protein A.", "contents": "Formation of extracellular protein A by Staphylococcus aureus. Staphylococcus aureus contains cell wall protein A as well as extracellular protein A. The two types of protein A have very similar amino acid compositions, electrophoretic mobilities and sizes. The release of extracellular protein A from exponentially growing bacteria is dependent on protein synthesis de novo and protein A is released directly after being synthesized on the ribosomes. Bacteria in the stationary phase, however, release protein A as a result of cell lysis. Protoplasts have been isolated which produce protein A as extensively as the intact bacteria but because of the absence of of the formation of extracellular protein A is observed from cells also producing cell wall protein A.", "PMID": 964266}
{"id": "pubmed23n0032_5858", "title": "Analysis of proteins synthesized in mitochondria of cultured mammalian cells. An assessment of current approaches and problems in interpretation.", "content": "1. The conditions which enable highly efficient utilization of [35S]methionine by cultured mammalian cells and the resolution of selectively labelled mitochondrial products are described. 2. Analysis of mitochondria purified from cells labelled in the presence or absence of inhibitors of cytoplasmic (or mitochondrial) protein synthesis indicated that about 5% of the [35S]methionine incorporated into mitochondrial proteins results from synthesis on mitoribosomes. 3. The electrophoretic profile of the detergent-solubilized proteins of mitochondrial isolated from cells which were labelled in the presence of 50 mug/ml emetine was similar to those obtained with extracts prepared by direct solbuilization of the intact cells after incorporation of label. 4. Pulse-labelling studies suggested that the components resolved by electrophoresis and autoradiography under the conditions described, apparently represent discrete and stable end products radiography under the conditions described, apparently represent discrete and stable end products of mitochondrial protein synthesis. No post-synthetic modification or degradation of these products was detected. 5. Erythromycin was found to suppress the synthesis of additional labelled products which were detected in extracts of one cell line, when analysed by procedures which normally detected only mitochondrially synthesized proteins. These additional bands were attributed to the synthetic activity of Mycoplasma.", "contents": "Analysis of proteins synthesized in mitochondria of cultured mammalian cells. An assessment of current approaches and problems in interpretation. 1. The conditions which enable highly efficient utilization of [35S]methionine by cultured mammalian cells and the resolution of selectively labelled mitochondrial products are described. 2. Analysis of mitochondria purified from cells labelled in the presence or absence of inhibitors of cytoplasmic (or mitochondrial) protein synthesis indicated that about 5% of the [35S]methionine incorporated into mitochondrial proteins results from synthesis on mitoribosomes. 3. The electrophoretic profile of the detergent-solubilized proteins of mitochondrial isolated from cells which were labelled in the presence of 50 mug/ml emetine was similar to those obtained with extracts prepared by direct solbuilization of the intact cells after incorporation of label. 4. Pulse-labelling studies suggested that the components resolved by electrophoresis and autoradiography under the conditions described, apparently represent discrete and stable end products radiography under the conditions described, apparently represent discrete and stable end products of mitochondrial protein synthesis. No post-synthetic modification or degradation of these products was detected. 5. Erythromycin was found to suppress the synthesis of additional labelled products which were detected in extracts of one cell line, when analysed by procedures which normally detected only mitochondrially synthesized proteins. These additional bands were attributed to the synthetic activity of Mycoplasma.", "PMID": 964267}
{"id": "pubmed23n0032_5859", "title": "Kinetic studies of the reactivity of the sulfhydryl groups of glyceraldehyde-3-phosphate dehydrogenase.", "content": "The reaction of sulfhydryl groups of glyceraldehyde-3-phosphate dehydrogenase from rabbit and pig muscles with a large molar excess of 5,5'-dithiobis(2-nitrobenzoate) (Nbs2) shows three-phasic pseudo-first-order kinetics. Since the fastest reaction between active cysteine-149 and Nbs2 is apparently biphasic, half-of-the-sites reactivity towards Nbs2 is suggested. Further sulfhydryl groups become reactive as an effect of conformational changes in the protein molecule after formation of a mixed disulfide on cysteine-149. In the presence of 40 mM borate the reaction is biphasic only, and two sulfhydryl groups per subunit react very quickly. The bound NAD+ is only partially released even after a long reaction with Nbs2. It was demonstrated that the two NAD+ binding sites with the highest dissociation constants have no significant effect on the reaction between cysteine-149 and Nbs2.", "contents": "Kinetic studies of the reactivity of the sulfhydryl groups of glyceraldehyde-3-phosphate dehydrogenase. The reaction of sulfhydryl groups of glyceraldehyde-3-phosphate dehydrogenase from rabbit and pig muscles with a large molar excess of 5,5'-dithiobis(2-nitrobenzoate) (Nbs2) shows three-phasic pseudo-first-order kinetics. Since the fastest reaction between active cysteine-149 and Nbs2 is apparently biphasic, half-of-the-sites reactivity towards Nbs2 is suggested. Further sulfhydryl groups become reactive as an effect of conformational changes in the protein molecule after formation of a mixed disulfide on cysteine-149. In the presence of 40 mM borate the reaction is biphasic only, and two sulfhydryl groups per subunit react very quickly. The bound NAD+ is only partially released even after a long reaction with Nbs2. It was demonstrated that the two NAD+ binding sites with the highest dissociation constants have no significant effect on the reaction between cysteine-149 and Nbs2.", "PMID": 964268}
{"id": "pubmed23n0032_5860", "title": "Diffusion of dextran in concentrated solutions.", "content": "A free-diffusion method has been developed for the determination of the intradiffusion coefficient ('self-diffusion coefficient') of a polymer in highly concentrated solutions. A fraction of the polymer is labelled with a small amount of light-absorbing substituent. The diffusion of this labelled species, present in low concentration, is followed in the presence of a high concentration of unlabelled material with the aid of absorption optics in the analytical ultracentrifuge. The diffusion proceeds over a boundary at which the difference in concentration of unlabelled material is varied. The average concentration of total polymer and the concentration of the labelled material are, however, constant. From theoretical considerations it is shown that by extrapolation of the diffusion coefficient so obtained to zero concentration difference of total material, the intradiffusion coefficient of the polymer at that concentration is obtained. The procedure also permits the ordinary translational diffusion coefficient to be estimated. The method has been applied to two dextran fractions with weight-average molecular weights of 19000 and 150000, which were labelled with fluorescein groups. As expected, the intradiffusion coefficient decreases with increasing polymer concentration, the decrease being more pronounced for the high-molecular-weight material. This decrease in the diffusion rate of dextran is, however, less than the corresponding decrease in the sedimentation rate which proteins with similar hydrodynamic parameters experience in dextran solutions. This agrees with the hypothesis that flexible linear polymers move through a network as chains rather than as hydrodynamic spheres. By combining measurements of the ordinary diffusion coefficient and the intradiffusion coefficient, it is possible to calculate the thermodynamic properties (as expressed by the virial expansion) of the system. This method is of particular importance in studies on concentrated solutions of high-molecular-weight polymers.", "contents": "Diffusion of dextran in concentrated solutions. A free-diffusion method has been developed for the determination of the intradiffusion coefficient ('self-diffusion coefficient') of a polymer in highly concentrated solutions. A fraction of the polymer is labelled with a small amount of light-absorbing substituent. The diffusion of this labelled species, present in low concentration, is followed in the presence of a high concentration of unlabelled material with the aid of absorption optics in the analytical ultracentrifuge. The diffusion proceeds over a boundary at which the difference in concentration of unlabelled material is varied. The average concentration of total polymer and the concentration of the labelled material are, however, constant. From theoretical considerations it is shown that by extrapolation of the diffusion coefficient so obtained to zero concentration difference of total material, the intradiffusion coefficient of the polymer at that concentration is obtained. The procedure also permits the ordinary translational diffusion coefficient to be estimated. The method has been applied to two dextran fractions with weight-average molecular weights of 19000 and 150000, which were labelled with fluorescein groups. As expected, the intradiffusion coefficient decreases with increasing polymer concentration, the decrease being more pronounced for the high-molecular-weight material. This decrease in the diffusion rate of dextran is, however, less than the corresponding decrease in the sedimentation rate which proteins with similar hydrodynamic parameters experience in dextran solutions. This agrees with the hypothesis that flexible linear polymers move through a network as chains rather than as hydrodynamic spheres. By combining measurements of the ordinary diffusion coefficient and the intradiffusion coefficient, it is possible to calculate the thermodynamic properties (as expressed by the virial expansion) of the system. This method is of particular importance in studies on concentrated solutions of high-molecular-weight polymers.", "PMID": 964269}
{"id": "pubmed23n0032_5861", "title": "Respiratory failure and cardiac disturbances in myotonic dystrophy.", "content": "Cardiopulmonary abnormalities are frequently encountered in myotonic dystrophy. We present five patients with myotonic dystrophy who entered the intensive care unit in acute respiratory failure. The possible etiologic factors of pulmonary complications are reviewed. The most important is probably aspiration pneumonia. The difficulties in the treatment of the respiratory failure are emphasezed. Myotonia of the chest muscles and diaphragm make artificial ventilation difficult. Recovery is delayed chiefly by swallowing disturbances. General anaesthesia is hazardous. Four patients presented cardiac arrhythmias and/or conduction abnormalities which were transient.", "contents": "Respiratory failure and cardiac disturbances in myotonic dystrophy. Cardiopulmonary abnormalities are frequently encountered in myotonic dystrophy. We present five patients with myotonic dystrophy who entered the intensive care unit in acute respiratory failure. The possible etiologic factors of pulmonary complications are reviewed. The most important is probably aspiration pneumonia. The difficulties in the treatment of the respiratory failure are emphasezed. Myotonia of the chest muscles and diaphragm make artificial ventilation difficult. Recovery is delayed chiefly by swallowing disturbances. General anaesthesia is hazardous. Four patients presented cardiac arrhythmias and/or conduction abnormalities which were transient.", "PMID": 964270}
{"id": "pubmed23n0032_5862", "title": "A nomogram to obtain pulmonary shuntflow (Qs/Qt).", "content": "A nomogram to simplify the determination of pulmonary shunt is presented. It is based on Leigh's et al. shunt-equation (1969). The nomogram aggregates the factors which contribute most to the pulmonary shunt in acute respiratory failure. Arterial and venous oxygen saturation, Hb content, arterial oxygen tension and barometric pressure do influence strongly the result of calculated shuntflow. For the factors with little importance in the calculation (vapor pressure, mixed venous oxygen tension, alveolar-CO2 tension), international mean values are used. The shunt determined with the nomogram deviates less than 1% from the calculated values, if the range of PCO2 is between 32 and 52 mm Hg and the error does not exceed 1,5% with a PCO2 up to 82 mm Hg unless it is combined with a very low Hb and a high mixed venous saturation. By the nomogram the effect of the different parameters on the shunt can be very well illustrated. Especially mistakes in determination of the mixed venous saturation and of arterial O2 tension result in errors. Too high saturation values in mixed venous blood occur in distally located catheters due to pulmonary capillary admixtures. This contamination can be easily detected if the relationship between PVO2 and PACO2 is examined. The other source of error lies in the technical problems of PO2 determination in high ranges and is caused by escape of O2 into the PO2-electrode and O2-consumption of the blood. The end effect which the factors of O2 escape have on the accuracy of PO2 determination in high ranges is demonstrated with blood samples which have been equilibrated with different gas mixtures at different temperatures and had their PO2 measured at regular intervals. The error in PaO2 measurement can be reduced if blood-gas analyses are performed immediately or on cooled samples.", "contents": "A nomogram to obtain pulmonary shuntflow (Qs/Qt). A nomogram to simplify the determination of pulmonary shunt is presented. It is based on Leigh's et al. shunt-equation (1969). The nomogram aggregates the factors which contribute most to the pulmonary shunt in acute respiratory failure. Arterial and venous oxygen saturation, Hb content, arterial oxygen tension and barometric pressure do influence strongly the result of calculated shuntflow. For the factors with little importance in the calculation (vapor pressure, mixed venous oxygen tension, alveolar-CO2 tension), international mean values are used. The shunt determined with the nomogram deviates less than 1% from the calculated values, if the range of PCO2 is between 32 and 52 mm Hg and the error does not exceed 1,5% with a PCO2 up to 82 mm Hg unless it is combined with a very low Hb and a high mixed venous saturation. By the nomogram the effect of the different parameters on the shunt can be very well illustrated. Especially mistakes in determination of the mixed venous saturation and of arterial O2 tension result in errors. Too high saturation values in mixed venous blood occur in distally located catheters due to pulmonary capillary admixtures. This contamination can be easily detected if the relationship between PVO2 and PACO2 is examined. The other source of error lies in the technical problems of PO2 determination in high ranges and is caused by escape of O2 into the PO2-electrode and O2-consumption of the blood. The end effect which the factors of O2 escape have on the accuracy of PO2 determination in high ranges is demonstrated with blood samples which have been equilibrated with different gas mixtures at different temperatures and had their PO2 measured at regular intervals. The error in PaO2 measurement can be reduced if blood-gas analyses are performed immediately or on cooled samples.", "PMID": 964271}
{"id": "pubmed23n0032_5863", "title": "Side effects of doxapram infusion.", "content": "Four cases are reported where patients reacted with severe restlessness, violence or hallucinations at low doses of doxapram infusion. A possible association with hepatic dysfunction is discussed. These reactions persisted long after the cessation of doxapram infusion and the various treatments used are described.", "contents": "Side effects of doxapram infusion. Four cases are reported where patients reacted with severe restlessness, violence or hallucinations at low doses of doxapram infusion. A possible association with hepatic dysfunction is discussed. These reactions persisted long after the cessation of doxapram infusion and the various treatments used are described.", "PMID": 964272}
{"id": "pubmed23n0032_5864", "title": "The use of tombramycin in the management of severe infections. Clinical and pharmacological data.", "content": "Tobramycin was used in the treatment of 35 severe infections. Its clinical effectiveness was confirmed in broncho-pulmonary infections without septicemia and in septicemia without lung involvement. Poor results were obtained in septicemia where the initial site 9 infection was in the lungs. This antibiotic appeared as a very good antistaphylococcal agent. In vitro superiority over gentamicin against Pseudomonas was not be confirmed clinically. Tobramycin deserves to be administered initially in serious infections because of the possibility that the causative organism might be a gentamicin-resistant, tobramycin susceptible strain. Three such cases were observed in our 35 patients. This susceptibility dissociation in favor of tobramycin was demonstrated in two strains of Klebsiella and one strain of Enterobacter. A dosage regimen in patients with impaired renal function is proposed. It requires confirmation.", "contents": "The use of tombramycin in the management of severe infections. Clinical and pharmacological data. Tobramycin was used in the treatment of 35 severe infections. Its clinical effectiveness was confirmed in broncho-pulmonary infections without septicemia and in septicemia without lung involvement. Poor results were obtained in septicemia where the initial site 9 infection was in the lungs. This antibiotic appeared as a very good antistaphylococcal agent. In vitro superiority over gentamicin against Pseudomonas was not be confirmed clinically. Tobramycin deserves to be administered initially in serious infections because of the possibility that the causative organism might be a gentamicin-resistant, tobramycin susceptible strain. Three such cases were observed in our 35 patients. This susceptibility dissociation in favor of tobramycin was demonstrated in two strains of Klebsiella and one strain of Enterobacter. A dosage regimen in patients with impaired renal function is proposed. It requires confirmation.", "PMID": 964273}
{"id": "pubmed23n0032_5865", "title": "Retrograde atrial preexcitation following premature ventricular beats during reciprocating tachycardia in the Wolff-Parkinson-White syndrome.", "content": "In 34 successive patients with Wolff-Parkinson-White syndrome premature beats were induced from the right ventricular apex during reciprocating tachycardia (RT) at progressively shorter coupling intervals. The presence of an accessory pathway was confirmed by a reduction in the atrial cycle length (A-A interval) during which the premature ventricular beat was introduced. This retrograde preexcitation occurred at a time when the His-AV node pathway was refractory; i.e. there was premature activation of the atria over a pathway other than the His-AV node. 3 patients were excluded because of unsatisfactory or unstable H-H intervals. In the remaining 31 patients with constant preceding H-H intervals, the A-A interval shortened; (a) 35-65 msec in 4 patients with right-sided pathways and normal conduction during RT and by 110 msec in a 5th patient with a right-sided pathway, in whom bundle branch block aberration persisted during RT, (b) 45 msec in the single patient with both a right-sided and a septal accessory pathway, (c) 35-65 msec in 5 patients with septal pathways, and (d) 15-35 msec in only 4/20 patients with left-sided pathways and normal conduction during RT. Left-sided ventricular premature beats were introduced in 5 patients with left-sided pathways and normal conduction in RT. In 4/5, left-sided premature beats shortened the A-A interval 40-75 msec whereas right-sided premature beats at the same coupling interval failed to do so. In the fifth case, the left-sided premature resulted in a 65 msec abbreviation of the A-A interval compared to 30 msec from the right ventricular outflow tract and 15 msec from the right ventricular apex. In 5 patients with left-sided pathways, right ventricular premature beats were introduced during RT with left bundle branch block aberration, and shortened the A-A interval 30-50 msec in all of these, whereas right-sided premature beats in 4 of the 5 during normal conduction failed to do so. This technique is useful to confirm the participation of accessory pathways in reciprocating tachycardias associated with the preexcitation syndromes, and emphasizes the importance of the site of stimulation used relative to the location of the accessory pathway. Because of the possibility of multiple accessory pathways, stimulation of the left ventricle should be performed in patients undergoing surgery for preexcitation unless the left ventricle is already inplicated by right-sided studies.", "contents": "Retrograde atrial preexcitation following premature ventricular beats during reciprocating tachycardia in the Wolff-Parkinson-White syndrome. In 34 successive patients with Wolff-Parkinson-White syndrome premature beats were induced from the right ventricular apex during reciprocating tachycardia (RT) at progressively shorter coupling intervals. The presence of an accessory pathway was confirmed by a reduction in the atrial cycle length (A-A interval) during which the premature ventricular beat was introduced. This retrograde preexcitation occurred at a time when the His-AV node pathway was refractory; i.e. there was premature activation of the atria over a pathway other than the His-AV node. 3 patients were excluded because of unsatisfactory or unstable H-H intervals. In the remaining 31 patients with constant preceding H-H intervals, the A-A interval shortened; (a) 35-65 msec in 4 patients with right-sided pathways and normal conduction during RT and by 110 msec in a 5th patient with a right-sided pathway, in whom bundle branch block aberration persisted during RT, (b) 45 msec in the single patient with both a right-sided and a septal accessory pathway, (c) 35-65 msec in 5 patients with septal pathways, and (d) 15-35 msec in only 4/20 patients with left-sided pathways and normal conduction during RT. Left-sided ventricular premature beats were introduced in 5 patients with left-sided pathways and normal conduction in RT. In 4/5, left-sided premature beats shortened the A-A interval 40-75 msec whereas right-sided premature beats at the same coupling interval failed to do so. In the fifth case, the left-sided premature resulted in a 65 msec abbreviation of the A-A interval compared to 30 msec from the right ventricular outflow tract and 15 msec from the right ventricular apex. In 5 patients with left-sided pathways, right ventricular premature beats were introduced during RT with left bundle branch block aberration, and shortened the A-A interval 30-50 msec in all of these, whereas right-sided premature beats in 4 of the 5 during normal conduction failed to do so. This technique is useful to confirm the participation of accessory pathways in reciprocating tachycardias associated with the preexcitation syndromes, and emphasizes the importance of the site of stimulation used relative to the location of the accessory pathway. Because of the possibility of multiple accessory pathways, stimulation of the left ventricle should be performed in patients undergoing surgery for preexcitation unless the left ventricle is already inplicated by right-sided studies.", "PMID": 964277}
{"id": "pubmed23n0032_5866", "title": "The retrograde His bundle deflection: its recognition and value in the analysis of tachyarrhythmias induced by stimulation on the T wave.", "content": "Interpretation of deflections presumably retrograde His bundle in origin has to be performed in context considering the coexisting changes in simultaneously recorded intracardiac and surface leads. His bundle electrocardiography thus conceived is helpful in the analysis of the runs of ectopic beats elicited by premature ventricular stimulation during the antecedent T wave in patients without coronary artery or primary myocardial disease. Identification of AV nodal echoes within the paroxysms as well as the subsequent runs of reciprocating tachycardias, was possible in patients with and without preexcitation, although a thorough study of these cases also requires recording of coronary sinus and low lateral right atrial electrograms. The behavior of the retrograde H deflection in respect to the first extra beat following the premature QRS complex helped in excluding bundle branch reentry. The latter is improbable in patients with 'complete' bundle branch block pattern, if extra beats show a contralateral bundle branch morphology. However, in absence of bundle branch block, retrograde H deflections were not helpful in elucidating the mechanisms of pacemaker-induced intraventricular (bundle branch, fasicular or vulnerability-related) reentry.", "contents": "The retrograde His bundle deflection: its recognition and value in the analysis of tachyarrhythmias induced by stimulation on the T wave. Interpretation of deflections presumably retrograde His bundle in origin has to be performed in context considering the coexisting changes in simultaneously recorded intracardiac and surface leads. His bundle electrocardiography thus conceived is helpful in the analysis of the runs of ectopic beats elicited by premature ventricular stimulation during the antecedent T wave in patients without coronary artery or primary myocardial disease. Identification of AV nodal echoes within the paroxysms as well as the subsequent runs of reciprocating tachycardias, was possible in patients with and without preexcitation, although a thorough study of these cases also requires recording of coronary sinus and low lateral right atrial electrograms. The behavior of the retrograde H deflection in respect to the first extra beat following the premature QRS complex helped in excluding bundle branch reentry. The latter is improbable in patients with 'complete' bundle branch block pattern, if extra beats show a contralateral bundle branch morphology. However, in absence of bundle branch block, retrograde H deflections were not helpful in elucidating the mechanisms of pacemaker-induced intraventricular (bundle branch, fasicular or vulnerability-related) reentry.", "PMID": 964278}
{"id": "pubmed23n0032_5867", "title": "A study of the acute electrophysiological and cardiovascular action of disopyramide in man.", "content": "The acute electrophysiological and hemodynamic effects of intravenous disopyramide (1.5 mg/kg body weight) were studied in 12 patients with suspected coronary artery disease. Plasma levels of disopyramide were monitored. Disopyramide delayed conduction within the His-Purkinje system and increased the effective refractory period of the atrium. Conduction within the AV node and its refractory period, however, was not significantly altered by the drug. The corrected Q-T interval was significantly increased. Disopyramide increased systemic arterial pressure and slightly raised the left ventricular end diastolic pressure. Heart rate, cardiac and stroke index were unaltered. Peak mean levels of disopyramide were reached 5 minutes after the injection of the drug.", "contents": "A study of the acute electrophysiological and cardiovascular action of disopyramide in man. The acute electrophysiological and hemodynamic effects of intravenous disopyramide (1.5 mg/kg body weight) were studied in 12 patients with suspected coronary artery disease. Plasma levels of disopyramide were monitored. Disopyramide delayed conduction within the His-Purkinje system and increased the effective refractory period of the atrium. Conduction within the AV node and its refractory period, however, was not significantly altered by the drug. The corrected Q-T interval was significantly increased. Disopyramide increased systemic arterial pressure and slightly raised the left ventricular end diastolic pressure. Heart rate, cardiac and stroke index were unaltered. Peak mean levels of disopyramide were reached 5 minutes after the injection of the drug.", "PMID": 964279}
{"id": "pubmed23n0032_5868", "title": "A new approach to simultaneous multiple unipolar and bipolar esophageal electrocardiograms, and the findings in normal sinus rhythm.", "content": "A multielectrode catheter for esophageal electrocardiography, using the cardia as point of fixation and reference, was tested in 10 normal adults. The ECG configurations derived from simultaneous multiple unipolar and bipolar leads in normal sinus rhythm are delineated. The bipolar recordings are particularly informative as they demonstrate the separate depolarization of each atrium, the distribution of atrial potentials in the sagittal plane with an obligatory shift in polarity at the midatrial level, and the pattern of left atrial depolarization. No previous report seems to have compiled these distinctive features.", "contents": "A new approach to simultaneous multiple unipolar and bipolar esophageal electrocardiograms, and the findings in normal sinus rhythm. A multielectrode catheter for esophageal electrocardiography, using the cardia as point of fixation and reference, was tested in 10 normal adults. The ECG configurations derived from simultaneous multiple unipolar and bipolar leads in normal sinus rhythm are delineated. The bipolar recordings are particularly informative as they demonstrate the separate depolarization of each atrium, the distribution of atrial potentials in the sagittal plane with an obligatory shift in polarity at the midatrial level, and the pattern of left atrial depolarization. No previous report seems to have compiled these distinctive features.", "PMID": 964280}
{"id": "pubmed23n0032_5869", "title": "Prinzmetal's variant angina: clinical, angiographic and pathologic correlations in two typical cases.", "content": "ECG and cinecoronary angiography of two patients with Prinzmetal's variant angina are reported. A pathologic study was performed on both. In one case, a transient luminal obstruction of the left circumflex coronary artery became apparent during selective coronary arteriography. Histologic examination of the corresponding coronary segment revealed a severe concentric atherosclerotic process with a normal-sized muscular layer, so that the angiographic transient occlusion might be attributed to an arterial spasm. In the second case no angiographic narrowings were demonstrated by cinecoronary angiography. Nevertheless, histologic examination revealed significant obstructive atherosclerotic narrowing in some coronary segments. It is suggested that atherosclerotic disease may be present also in cases with variant angina who have apparently normal coronary angiograms.", "contents": "Prinzmetal's variant angina: clinical, angiographic and pathologic correlations in two typical cases. ECG and cinecoronary angiography of two patients with Prinzmetal's variant angina are reported. A pathologic study was performed on both. In one case, a transient luminal obstruction of the left circumflex coronary artery became apparent during selective coronary arteriography. Histologic examination of the corresponding coronary segment revealed a severe concentric atherosclerotic process with a normal-sized muscular layer, so that the angiographic transient occlusion might be attributed to an arterial spasm. In the second case no angiographic narrowings were demonstrated by cinecoronary angiography. Nevertheless, histologic examination revealed significant obstructive atherosclerotic narrowing in some coronary segments. It is suggested that atherosclerotic disease may be present also in cases with variant angina who have apparently normal coronary angiograms.", "PMID": 964281}
{"id": "pubmed23n0032_5870", "title": "The grip test: a simple method for the assessment of left ventricular performance.", "content": "37 patients with mixed cardiac pathologies were subjected to isometric exercise (hand grip) during routine cardiac catheterization. On the basis of a simple and safe grip test it was possible to distinguish three groups of patients according to the left ventricular pressure at rest and its response to this test. Group 1 consisted of 14 patients with left ventricular end diastolic pressures remaining below 12 mm Hg both at rest and on exercise. These patients were considered to have normal left ventricular function some, even in the presence of organic heart disease. No deaths occurred in this group during the follow-up period which averaged 33.8 months. At the other extreme (Group 3) there were 12 obviously disabled patients with resting left ventricular filling pressures above 12 mm Hg rising further under isometric stress. Six of these patients (50%) died during the period of the study. (Average follow-up 21.4 months). By the application of the hand grip test, an intermediate population (Group 2) of 11 patients was discernible. These patients were able to maintain a normal cardiac reserve at rest (LVEDP less than 12 mm Hg) but not during isometric effort (LVEDP greater than 12 mm Hg). Two of these patients (18%) died during the follow up period (average 22.1 months). Assuming a pathological progression with time from groups 1-3 and in view of the different prognoses observed in the course of the long-term follow-up it would appear that the Group 2 patients should be considered more critically and offered more active management.", "contents": "The grip test: a simple method for the assessment of left ventricular performance. 37 patients with mixed cardiac pathologies were subjected to isometric exercise (hand grip) during routine cardiac catheterization. On the basis of a simple and safe grip test it was possible to distinguish three groups of patients according to the left ventricular pressure at rest and its response to this test. Group 1 consisted of 14 patients with left ventricular end diastolic pressures remaining below 12 mm Hg both at rest and on exercise. These patients were considered to have normal left ventricular function some, even in the presence of organic heart disease. No deaths occurred in this group during the follow-up period which averaged 33.8 months. At the other extreme (Group 3) there were 12 obviously disabled patients with resting left ventricular filling pressures above 12 mm Hg rising further under isometric stress. Six of these patients (50%) died during the period of the study. (Average follow-up 21.4 months). By the application of the hand grip test, an intermediate population (Group 2) of 11 patients was discernible. These patients were able to maintain a normal cardiac reserve at rest (LVEDP less than 12 mm Hg) but not during isometric effort (LVEDP greater than 12 mm Hg). Two of these patients (18%) died during the follow up period (average 22.1 months). Assuming a pathological progression with time from groups 1-3 and in view of the different prognoses observed in the course of the long-term follow-up it would appear that the Group 2 patients should be considered more critically and offered more active management.", "PMID": 964282}
{"id": "pubmed23n0032_5871", "title": "Familial prevalence of asymmetric septal hypertrophy.", "content": "Echocardiography was used to detect the familial prevalence of asymmetric septal hypertrophy in relatives of patients with proven idiopathic hypertrophic subaortic stenosis. Idiopathic hypertrophic subaortic stenosis is only one clinical expression of a cardiomyopathic disease spectrum, including asymptomatic patients having asymmetric septal hypertrophy as a characteristic anatomic marker which can be detected by echo. The validity of previous proposed criteria to detect this marker was checked in our population. Therefore we examined normal subjects, patients with fixed left ventricular outflow obstruction (valvular aortic stenosis), and those with idiopathic hypertrophic subaortic stenosis who served as index cases. A septal thickness exceeding that of the free left ventricular posterior wall by 30% separates patients with a cardiomyopathy from those without this disease. 27 of 73 examined relatives of 14 index cases were found to have asymmetric septal hypertrophy. In those instances where information was available from the parents of the index cases, one parent was found to be affected. When the examined group is considered from a parent-child relationship (including the index case when appropriate), it included 78 children of affected single parents of which 20 males and 19 females had asymmetric septal hypertrophy. The history, clinical examination and electrocardiogram were not useful to detect the disease. The results suggest an autosomal dominant mode of inheritance of asymmetric septal hypertrophy with a high penetrance.", "contents": "Familial prevalence of asymmetric septal hypertrophy. Echocardiography was used to detect the familial prevalence of asymmetric septal hypertrophy in relatives of patients with proven idiopathic hypertrophic subaortic stenosis. Idiopathic hypertrophic subaortic stenosis is only one clinical expression of a cardiomyopathic disease spectrum, including asymptomatic patients having asymmetric septal hypertrophy as a characteristic anatomic marker which can be detected by echo. The validity of previous proposed criteria to detect this marker was checked in our population. Therefore we examined normal subjects, patients with fixed left ventricular outflow obstruction (valvular aortic stenosis), and those with idiopathic hypertrophic subaortic stenosis who served as index cases. A septal thickness exceeding that of the free left ventricular posterior wall by 30% separates patients with a cardiomyopathy from those without this disease. 27 of 73 examined relatives of 14 index cases were found to have asymmetric septal hypertrophy. In those instances where information was available from the parents of the index cases, one parent was found to be affected. When the examined group is considered from a parent-child relationship (including the index case when appropriate), it included 78 children of affected single parents of which 20 males and 19 females had asymmetric septal hypertrophy. The history, clinical examination and electrocardiogram were not useful to detect the disease. The results suggest an autosomal dominant mode of inheritance of asymmetric septal hypertrophy with a high penetrance.", "PMID": 964283}
{"id": "pubmed23n0032_5872", "title": "Effect of transducer placement on echocardiographic mitral valve systolic motion.", "content": "The influence of transducer position and angulation upon the mitral systolic echo was studied in 100 presumably healthy females. Echocardiographic studies were performed from the second, third, fourth and fifth intercostal spaces (ICS). The role of the sound beam's path relative to cardiac motion was assessed by analyzing the recorded mitral valve pattern as a function of transducer orientation, independent of the absolute ICS used. With the transducer directed caudally when both mitral leaflets and left atrium were recorded, holosystolic or midsystolic posterior motion of the mitral valve leaflet echo was seen in 59% of the subjects. These patterns, recorded this way, were not related to phonocardiographic signs suggesting mitral valve prolapse. Best correlation with phonocardiographic findings was obtained when the echocardiographic examination was performed with the transducer either perpendicular to the chest in the sagittal plane, or pointing slightly cephalad ('perpendicular' position). With the transducer in 'perpendicular' position, both holosystolic and midsystolic posterior motion of the mitral systolic echo, deviating more than 2 mm from a line joining the C and D points, were highly related statistically to phonocardiographic findings suggesting mitral valve prolapse. This study demonstrates that transducer position and angulation on the chest wall are important determinants of echocardiographic appearance of mitral valve during systole. Only the 'perpendicular' transducer position should be used when analyzing echocardiograms for the presence of mitral valve prolapse.", "contents": "Effect of transducer placement on echocardiographic mitral valve systolic motion. The influence of transducer position and angulation upon the mitral systolic echo was studied in 100 presumably healthy females. Echocardiographic studies were performed from the second, third, fourth and fifth intercostal spaces (ICS). The role of the sound beam's path relative to cardiac motion was assessed by analyzing the recorded mitral valve pattern as a function of transducer orientation, independent of the absolute ICS used. With the transducer directed caudally when both mitral leaflets and left atrium were recorded, holosystolic or midsystolic posterior motion of the mitral valve leaflet echo was seen in 59% of the subjects. These patterns, recorded this way, were not related to phonocardiographic signs suggesting mitral valve prolapse. Best correlation with phonocardiographic findings was obtained when the echocardiographic examination was performed with the transducer either perpendicular to the chest in the sagittal plane, or pointing slightly cephalad ('perpendicular' position). With the transducer in 'perpendicular' position, both holosystolic and midsystolic posterior motion of the mitral systolic echo, deviating more than 2 mm from a line joining the C and D points, were highly related statistically to phonocardiographic findings suggesting mitral valve prolapse. This study demonstrates that transducer position and angulation on the chest wall are important determinants of echocardiographic appearance of mitral valve during systole. Only the 'perpendicular' transducer position should be used when analyzing echocardiograms for the presence of mitral valve prolapse.", "PMID": 964284}
{"id": "pubmed23n0032_5873", "title": "Exercise performance in children and adolescents after surgical repair of tetralogy of Fallot.", "content": "Cardiopulmonary function studies at rest and during submaximal and maximal exercise were performed in 21 children and adolescents who had undergone surgical correction of tetralogy of Fallot. Maximal oxygen uptake of the patients was 84.6% of healthy peers matched for age and height. The reduced aerobic capacity can mainly be attributed to a reduction in stroke volume. In the presence of a reduced stroke volume normal cardiac output during submaximal exercise was achieved and maintained by an increase in heart rate. During maximal exercise, however, the heart rate did not exceed that of the healthy controls and the results for the children in this series are about 20% higher than those reported in the literature for adults who had undergone surgical repair of a tetralogy. Persistent impairment of cardiac function in patients with tetralogy of Fallot who have undergone corrective surgery may represent a residual outflow tract obstruction in the right ventricle, impaired function of the left ventricle or the result of restricted physical activity.", "contents": "Exercise performance in children and adolescents after surgical repair of tetralogy of Fallot. Cardiopulmonary function studies at rest and during submaximal and maximal exercise were performed in 21 children and adolescents who had undergone surgical correction of tetralogy of Fallot. Maximal oxygen uptake of the patients was 84.6% of healthy peers matched for age and height. The reduced aerobic capacity can mainly be attributed to a reduction in stroke volume. In the presence of a reduced stroke volume normal cardiac output during submaximal exercise was achieved and maintained by an increase in heart rate. During maximal exercise, however, the heart rate did not exceed that of the healthy controls and the results for the children in this series are about 20% higher than those reported in the literature for adults who had undergone surgical repair of a tetralogy. Persistent impairment of cardiac function in patients with tetralogy of Fallot who have undergone corrective surgery may represent a residual outflow tract obstruction in the right ventricle, impaired function of the left ventricle or the result of restricted physical activity.", "PMID": 964285}
{"id": "pubmed23n0032_5874", "title": "Pharmacokinetics of diazepam in disordered liver function.", "content": "The plasma elimination curves of diazepam following intravenous administration of 10 mg were studied in nine patients with cirrhosis of the liver and four patients without liver disease. The data were analyzed according to a two compartment model. The mean biological half-life (T/2) of diazepam was increased five-fold in patients with cirrhosis compared to the controls (164 hours vs. 32.1 hours). The plasma clearance of diazepam could be correlated neither with a quantitative measure of liver function, as estimated by galactose elimination capacity, nor to semiquantitative measures of liver function, such as serum albumin and prothrombin. It is suggested that the plasma clearance of diazepam is an inaccurate index of its rate of hepatic metabolism due to the complex kinetics of the drug.", "contents": "Pharmacokinetics of diazepam in disordered liver function. The plasma elimination curves of diazepam following intravenous administration of 10 mg were studied in nine patients with cirrhosis of the liver and four patients without liver disease. The data were analyzed according to a two compartment model. The mean biological half-life (T/2) of diazepam was increased five-fold in patients with cirrhosis compared to the controls (164 hours vs. 32.1 hours). The plasma clearance of diazepam could be correlated neither with a quantitative measure of liver function, as estimated by galactose elimination capacity, nor to semiquantitative measures of liver function, such as serum albumin and prothrombin. It is suggested that the plasma clearance of diazepam is an inaccurate index of its rate of hepatic metabolism due to the complex kinetics of the drug.", "PMID": 964287}
{"id": "pubmed23n0032_5875", "title": "Comparison of the pharmacokinetics of diazepam after single and subchronic doses.", "content": "In seven healthy male volunteers the effects of the pattern of dosing on the pharmacokinetics of diazepam have been studied. A cross-over design was employed that consisted of three parts: a single intravenous dose (0.1 mg/kg), and oral dosing (10 mg/day) for six days followed by an intravenous bolus (0.1 mg/kg) on the seventh day, followed by re-examination of a single intravenous dose after diazepam (D) and its major metabolite desmethyldiazepam (DD) had been completely eliminated. Plasma levels of D and DD were monitored by a specific, sensitive GLC-method. In younger patients (n = 5, age 29 - 35 years) the elimination half-life, T1/2(beta), of D was 33.9 +/- 10.6 h (mean +/- S.D.) after the single dose. The control study gave an almost identical result (35.7 +/- 12.1). After subchronic dosage in all patients T1/2(beta) showed a modest but significant prolongation (paired t-test p less than 0.01) to 52.9 +/- 17.4 h. It was caused by a significant decrease (p = 0.016) in total plasma clearance (Cl), from 26.0 +/- 10.8 ml/min. Older patients (age 43-60 years) showed the same phenomenon. Blood/plasma ratios remained constant indicating no change in protein binding. Biliary excretion of D was measured in five patients with a T-tube. Only negligible amounts (0.3 - 0.4%) of administered D were excreted within 3 days after subchronic dosage, which demonstrates a lack of enterohepatic cycling of D. After multiple administration of D, there was accumulation of DD to levels approximately five times higher than after a single dose. The possibility that the slower elimination of D after subchronic treatment might be caused by DD was also supported by experiments in dogs and rabbits. After pretreating rabbits with DD and maintaining a high DD plasma level, there was prolongation of T1/2(beta) from 2.7 h to 5.2 h, with a corresponding decrease of Cl from 101.6 ml/min to 23.4 ml/min. Similar results were obtained in dogs. It is concluded that the disposition of D is altered by subchronic use and may be regulated by the plasma DD concentration.", "contents": "Comparison of the pharmacokinetics of diazepam after single and subchronic doses. In seven healthy male volunteers the effects of the pattern of dosing on the pharmacokinetics of diazepam have been studied. A cross-over design was employed that consisted of three parts: a single intravenous dose (0.1 mg/kg), and oral dosing (10 mg/day) for six days followed by an intravenous bolus (0.1 mg/kg) on the seventh day, followed by re-examination of a single intravenous dose after diazepam (D) and its major metabolite desmethyldiazepam (DD) had been completely eliminated. Plasma levels of D and DD were monitored by a specific, sensitive GLC-method. In younger patients (n = 5, age 29 - 35 years) the elimination half-life, T1/2(beta), of D was 33.9 +/- 10.6 h (mean +/- S.D.) after the single dose. The control study gave an almost identical result (35.7 +/- 12.1). After subchronic dosage in all patients T1/2(beta) showed a modest but significant prolongation (paired t-test p less than 0.01) to 52.9 +/- 17.4 h. It was caused by a significant decrease (p = 0.016) in total plasma clearance (Cl), from 26.0 +/- 10.8 ml/min. Older patients (age 43-60 years) showed the same phenomenon. Blood/plasma ratios remained constant indicating no change in protein binding. Biliary excretion of D was measured in five patients with a T-tube. Only negligible amounts (0.3 - 0.4%) of administered D were excreted within 3 days after subchronic dosage, which demonstrates a lack of enterohepatic cycling of D. After multiple administration of D, there was accumulation of DD to levels approximately five times higher than after a single dose. The possibility that the slower elimination of D after subchronic treatment might be caused by DD was also supported by experiments in dogs and rabbits. After pretreating rabbits with DD and maintaining a high DD plasma level, there was prolongation of T1/2(beta) from 2.7 h to 5.2 h, with a corresponding decrease of Cl from 101.6 ml/min to 23.4 ml/min. Similar results were obtained in dogs. It is concluded that the disposition of D is altered by subchronic use and may be regulated by the plasma DD concentration.", "PMID": 964288}
{"id": "pubmed23n0032_5876", "title": "Coumarin-7-hydroxylase activity in microsomes from needle biopsies of normal and diseased human liver.", "content": "The specific activity of coumarin-7-hydroxylase was measured in liver microsomes from normal subjects and patients with liver disease. Liver specimens were obtained by needle biopsy and the microsomal fraction was separated by differential centrifugation. Its freedom from mitochondria was demonstrated by the absence of succinic dehydrogenase, a marker enzyme for mitochondria. Liver from healthy subjects showed variation in the specific activity of coumarin-7-hydroxylase from 0.16 to 0.65 nmol-mg-1-min-1, which is probably due to genetic factors. Patients with cirrhosis of the liver, chronic fatty hepatitis (chronic alcoholic hepatitis) and chronic active hepatitis showed a significantly lower mean hydroxylase activity. There was no significant difference in the mean level of hydroxylase between patients with subacute viral hepatitis or chronic persistent hepatitis and the normal controls.", "contents": "Coumarin-7-hydroxylase activity in microsomes from needle biopsies of normal and diseased human liver. The specific activity of coumarin-7-hydroxylase was measured in liver microsomes from normal subjects and patients with liver disease. Liver specimens were obtained by needle biopsy and the microsomal fraction was separated by differential centrifugation. Its freedom from mitochondria was demonstrated by the absence of succinic dehydrogenase, a marker enzyme for mitochondria. Liver from healthy subjects showed variation in the specific activity of coumarin-7-hydroxylase from 0.16 to 0.65 nmol-mg-1-min-1, which is probably due to genetic factors. Patients with cirrhosis of the liver, chronic fatty hepatitis (chronic alcoholic hepatitis) and chronic active hepatitis showed a significantly lower mean hydroxylase activity. There was no significant difference in the mean level of hydroxylase between patients with subacute viral hepatitis or chronic persistent hepatitis and the normal controls.", "PMID": 964289}
{"id": "pubmed23n0032_5877", "title": "Binding of four sulphonamides to human albumin.", "content": "The four sulphonamides studied--furosemide, tolbutamide, sulfafurazole and sulfonamidochlorobenzoic acid--bind to human albumin at the same sites but with decreasing affinity. These sites are also common to other drugs, namely acenocoumarin, chlorophenoxyisobutyric acid, phenylbutazone and warfarin. In plasma, the four sulphonamides considered bind mainly to albumin, but also, at higher concentrations, to globulins, to an extent that increases as their affinity for albumin lessens.", "contents": "Binding of four sulphonamides to human albumin. The four sulphonamides studied--furosemide, tolbutamide, sulfafurazole and sulfonamidochlorobenzoic acid--bind to human albumin at the same sites but with decreasing affinity. These sites are also common to other drugs, namely acenocoumarin, chlorophenoxyisobutyric acid, phenylbutazone and warfarin. In plasma, the four sulphonamides considered bind mainly to albumin, but also, at higher concentrations, to globulins, to an extent that increases as their affinity for albumin lessens.", "PMID": 964290}
{"id": "pubmed23n0032_5878", "title": "Pharmacokinetics and pharmacodynamics of alprenolol in the treatment of hypertension. II. Relationship to its effect on blood pressure and plasma renin activity.", "content": "The kinetics of alprenolol, in relation to its effect on blood pressure and plasma renin activity, have been studied in sixteen patients. A within-patient comparison was made between therapy for six weeks with placebo or alpronolol 200 mg thrice daily. Thirteen patients responded to alprenolol by a signoficant fall in blood pressure. In three other patients treatment did not lower blood pressure. In the group as a whole there was no significant correlation between the fall in systolic, diastolic or mean blood pressure, and the steady-state plasma alprenolol concentration, renin status, or degree of beta blockade. However, the thirteen responsive patients showed a significant relationship (p less than 0.05 - 0.001) between the log mean steady-state plasma alprenolol concentration and the hypotensive response.", "contents": "Pharmacokinetics and pharmacodynamics of alprenolol in the treatment of hypertension. II. Relationship to its effect on blood pressure and plasma renin activity. The kinetics of alprenolol, in relation to its effect on blood pressure and plasma renin activity, have been studied in sixteen patients. A within-patient comparison was made between therapy for six weeks with placebo or alpronolol 200 mg thrice daily. Thirteen patients responded to alprenolol by a signoficant fall in blood pressure. In three other patients treatment did not lower blood pressure. In the group as a whole there was no significant correlation between the fall in systolic, diastolic or mean blood pressure, and the steady-state plasma alprenolol concentration, renin status, or degree of beta blockade. However, the thirteen responsive patients showed a significant relationship (p less than 0.05 - 0.001) between the log mean steady-state plasma alprenolol concentration and the hypotensive response.", "PMID": 964292}
{"id": "pubmed23n0032_5879", "title": "Serum digoxin levels in patients of a general practice in Germany.", "content": "Serum digoxin levels were determined in 33 outpatients of a general practice in the countryside, on three occasions at intervals of 8 weeks. All the patients were on long term digoxin treatment, about 2 years on average. About 14 days after the first and the second visits the results of the measurements were sent to the patients, with a comment about their reliability in taking treatment according to the serum digoxin level. At the first visit half of the serum digoxin level were lower than 0.5 ng/ml; the mean serum concentration was 0.52 ng/ml. There was no correlation between serum concentration and age, dose or creatinine level; but there was with replies to the question about regularity of drug intake. The mean serum level at the second and the third visits was 0.88 ng/ml and 0.89 ng/ml, respectively. A correlation was found between the dose and the serum digoxin level. From these results it seems that compliance by the patient plays a major role in producing steady state levels of drugs.", "contents": "Serum digoxin levels in patients of a general practice in Germany. Serum digoxin levels were determined in 33 outpatients of a general practice in the countryside, on three occasions at intervals of 8 weeks. All the patients were on long term digoxin treatment, about 2 years on average. About 14 days after the first and the second visits the results of the measurements were sent to the patients, with a comment about their reliability in taking treatment according to the serum digoxin level. At the first visit half of the serum digoxin level were lower than 0.5 ng/ml; the mean serum concentration was 0.52 ng/ml. There was no correlation between serum concentration and age, dose or creatinine level; but there was with replies to the question about regularity of drug intake. The mean serum level at the second and the third visits was 0.88 ng/ml and 0.89 ng/ml, respectively. A correlation was found between the dose and the serum digoxin level. From these results it seems that compliance by the patient plays a major role in producing steady state levels of drugs.", "PMID": 964293}
{"id": "pubmed23n0032_5880", "title": "Studies on the lymphocytes of sheep. I. Recirculation of lymphocytes through peripheral lymph nodes and tissues.", "content": "Recirculating lymphocytes were collected from the efferent ducts of peripheral somatic nodes and incubated in vitro with 51Cr. After washing, the labeled cells were returned to the sheep by i.v. injection. The partitioning of the labeled cells between blood and efferent (intermediate) lymph, or between efferent and afferent (peripheral) lymph was monitored for the next 24 h by assaying the radioactivity in appropriate samples of cells. The labeled lymphocytes took several hours to migrate through the nodes so that they equilibrated between blood and lymph in about 10 h. This pattern was not altered grossly during the inductive phase of an immune response. The small numbers of lymphocytes which migrated through peripheral tissues entered the lymph at least quickly as those that migrated through lymph nodes.", "contents": "Studies on the lymphocytes of sheep. I. Recirculation of lymphocytes through peripheral lymph nodes and tissues. Recirculating lymphocytes were collected from the efferent ducts of peripheral somatic nodes and incubated in vitro with 51Cr. After washing, the labeled cells were returned to the sheep by i.v. injection. The partitioning of the labeled cells between blood and efferent (intermediate) lymph, or between efferent and afferent (peripheral) lymph was monitored for the next 24 h by assaying the radioactivity in appropriate samples of cells. The labeled lymphocytes took several hours to migrate through the nodes so that they equilibrated between blood and lymph in about 10 h. This pattern was not altered grossly during the inductive phase of an immune response. The small numbers of lymphocytes which migrated through peripheral tissues entered the lymph at least quickly as those that migrated through lymph nodes.", "PMID": 964294}
{"id": "pubmed23n0032_5881", "title": "Suppressor cells in rabbit peripheral blood.", "content": "Rabbit peripheral blood leukocytes (PBL) added to cultures of autologous spleen cells, primed in vivo to sheep red cells, are able to suppress with high efficacy the secondary in vitro response of the spleen cells to that antigen. Removal of nylon wool adherent cells from PBL abolishes the suppressive effect. When the PBL are fractionated by velocity sedimentation the suppressor cells can be separated from the responding cells. The circulating lymphocytes, freed from the inhibiting effect, either by nylon wool absorption or by velocity sedimentation fractionation, are able to give a strong secondary in vitro anti-SRC response, in which the long latency period, usually observed when PBL are stimulated with antigen in culture, is abolished or at least reduced. The suppressor effect present in the PBL is not due to granulocytes, platelets or erythrocytes.", "contents": "Suppressor cells in rabbit peripheral blood. Rabbit peripheral blood leukocytes (PBL) added to cultures of autologous spleen cells, primed in vivo to sheep red cells, are able to suppress with high efficacy the secondary in vitro response of the spleen cells to that antigen. Removal of nylon wool adherent cells from PBL abolishes the suppressive effect. When the PBL are fractionated by velocity sedimentation the suppressor cells can be separated from the responding cells. The circulating lymphocytes, freed from the inhibiting effect, either by nylon wool absorption or by velocity sedimentation fractionation, are able to give a strong secondary in vitro anti-SRC response, in which the long latency period, usually observed when PBL are stimulated with antigen in culture, is abolished or at least reduced. The suppressor effect present in the PBL is not due to granulocytes, platelets or erythrocytes.", "PMID": 964295}
{"id": "pubmed23n0032_5882", "title": "Ontogeny of adherent cells. I. Distribution and ontogeny of A cells participating in the response to sheep erythrocytes in vitro.", "content": "An adherent (A) cell function required for the in vitro antibody-forming response to sheep erythrocyte (SRBC) antigen has been demonstrated in a culture medium enriched with nucleic acid precursors and 2-mercaptoethanol. The essential function is fulfilled best by adult adherent cells of the spleen, to some extent by bone marrow and perhaps lymph node A cells, and poorly or not at all by peritoneal or lung A cells. Ontogenetic studies reveal the absence or inhibition of neonatal A cell synergistic function in the in vitro response to SRBC. The capacity of splenic adherent cells to collaborate with column-separated nonadherent cells appears only from the third or fourth day after birth, with ratio indices reaching levels significantly greater than nonsynergistic background by the fifth day and showing little further increase until the second week of age.", "contents": "Ontogeny of adherent cells. I. Distribution and ontogeny of A cells participating in the response to sheep erythrocytes in vitro. An adherent (A) cell function required for the in vitro antibody-forming response to sheep erythrocyte (SRBC) antigen has been demonstrated in a culture medium enriched with nucleic acid precursors and 2-mercaptoethanol. The essential function is fulfilled best by adult adherent cells of the spleen, to some extent by bone marrow and perhaps lymph node A cells, and poorly or not at all by peritoneal or lung A cells. Ontogenetic studies reveal the absence or inhibition of neonatal A cell synergistic function in the in vitro response to SRBC. The capacity of splenic adherent cells to collaborate with column-separated nonadherent cells appears only from the third or fourth day after birth, with ratio indices reaching levels significantly greater than nonsynergistic background by the fifth day and showing little further increase until the second week of age.", "PMID": 964296}
{"id": "pubmed23n0032_5883", "title": "Cell surface immunoglobulin. XV. The presence of IgM and an IgD-like molecule on the same cell in murine lymphoid tissue.", "content": "The present studies were designed to determine whether IgM and \"IgD\" are on the same cell in the mouse. Thus, radioiodinated murine lymphocytes were treated with anti-mu and complement to kill IgM-bearing cells. The dead cells were removed by gradient centrifugation. Results indicate that a portion of the IgD is removed by depleting IgM-bearing cells. Using cells from thoracic duct or lymph nodes but not spleen, it was evident that some of the double bearers are large lymphocytes.", "contents": "Cell surface immunoglobulin. XV. The presence of IgM and an IgD-like molecule on the same cell in murine lymphoid tissue. The present studies were designed to determine whether IgM and \"IgD\" are on the same cell in the mouse. Thus, radioiodinated murine lymphocytes were treated with anti-mu and complement to kill IgM-bearing cells. The dead cells were removed by gradient centrifugation. Results indicate that a portion of the IgD is removed by depleting IgM-bearing cells. Using cells from thoracic duct or lymph nodes but not spleen, it was evident that some of the double bearers are large lymphocytes.", "PMID": 964297}
{"id": "pubmed23n0032_5884", "title": "Concentrations of three hapten-binding immunoglobulins in pooled normal human serum.", "content": "The amount of natural anti-DNP-cap, anti-NIP-cap or anti-ABA-TYR in human IgM was measured by using immunoabsorbents and hapten elution. We found that 1.0% of pooled human IgM is specific for DNP-cap, 0.2% for NIP-cap and 0.02% for ABA-TYR. Haptens NIP and DNP were found to be cross-reactive when tested with natural antibodies even though they were not cross-reactive when tested with immune antibodies.", "contents": "Concentrations of three hapten-binding immunoglobulins in pooled normal human serum. The amount of natural anti-DNP-cap, anti-NIP-cap or anti-ABA-TYR in human IgM was measured by using immunoabsorbents and hapten elution. We found that 1.0% of pooled human IgM is specific for DNP-cap, 0.2% for NIP-cap and 0.02% for ABA-TYR. Haptens NIP and DNP were found to be cross-reactive when tested with natural antibodies even though they were not cross-reactive when tested with immune antibodies.", "PMID": 964298}
{"id": "pubmed23n0032_5885", "title": "The effects of dantrolene sodium on cardiac and skeletal muscle in rats.", "content": "Dantrolene sodium in different concentrations was administered to the spontaneously beating heart placed in a modified Langendorff apparatus. Heart frequency and contractility were recorded. Dantrolene sodium was also administered to the rat diaphragm. Twitch tension after indirect supramaximal stimulation was recorded. Dantrolene sodium produced a long lasting dose-dependent reduction of the contractility of the isolated rat heart up to 75% of control values. It had no effect on the heart frequency. The drug also decreased the force of contraction of the rat skeletal muscle in vitro to the same extent. The diaphragm appeared to be more sensitive to low concentrations of dantrolene sodium than was heart muscle i.e. the dose-response curve on rat diaphragm was flatter. It may be concluded however that higher concentrations of dantrolene sodium may effect the contractility of heart muscle as well and that this may have clinical implications.", "contents": "The effects of dantrolene sodium on cardiac and skeletal muscle in rats. Dantrolene sodium in different concentrations was administered to the spontaneously beating heart placed in a modified Langendorff apparatus. Heart frequency and contractility were recorded. Dantrolene sodium was also administered to the rat diaphragm. Twitch tension after indirect supramaximal stimulation was recorded. Dantrolene sodium produced a long lasting dose-dependent reduction of the contractility of the isolated rat heart up to 75% of control values. It had no effect on the heart frequency. The drug also decreased the force of contraction of the rat skeletal muscle in vitro to the same extent. The diaphragm appeared to be more sensitive to low concentrations of dantrolene sodium than was heart muscle i.e. the dose-response curve on rat diaphragm was flatter. It may be concluded however that higher concentrations of dantrolene sodium may effect the contractility of heart muscle as well and that this may have clinical implications.", "PMID": 964299}
{"id": "pubmed23n0032_5886", "title": "Increase in the coronary vascular resistance by indomethacin in the isolated guinea pig heart preparation in the absence of changes in mechanical performance and oxygen consumption.", "content": "Experiments were performed in isolated guinea pig hearts, perfused at constant volume. Left ventricular pressure, left ventricular dp/dtmax, coronary vascular pressure and coronary venous pO2 were measured continuously. Indomethacin (1.4 X 10(-6) M) increased coronary vascular resistance by 15% over control (p less than 0.01) without altering the myocardial contractile force. When PGE2 (1.4 X10(-8) M) was subsequently added, it abolished the indomethacin action and lowered the coronary vascular resistance below the pre-indomethacin control value. This increase in coronary vascular resistance caused by indomethacin was also seen when the animals were pretreated with reserpine and/or the adrenoceptor blocking agents phenoxybenzamine and propranolol, but was prevented completely by previous addition of PGE2 (1.4 X 10(-7) M). The results provide evidence for possible involvement of endogenous prostaglandin-like substances in the maintenance of coronary vascular resistance in the isolated guinea pig heart.", "contents": "Increase in the coronary vascular resistance by indomethacin in the isolated guinea pig heart preparation in the absence of changes in mechanical performance and oxygen consumption. Experiments were performed in isolated guinea pig hearts, perfused at constant volume. Left ventricular pressure, left ventricular dp/dtmax, coronary vascular pressure and coronary venous pO2 were measured continuously. Indomethacin (1.4 X 10(-6) M) increased coronary vascular resistance by 15% over control (p less than 0.01) without altering the myocardial contractile force. When PGE2 (1.4 X10(-8) M) was subsequently added, it abolished the indomethacin action and lowered the coronary vascular resistance below the pre-indomethacin control value. This increase in coronary vascular resistance caused by indomethacin was also seen when the animals were pretreated with reserpine and/or the adrenoceptor blocking agents phenoxybenzamine and propranolol, but was prevented completely by previous addition of PGE2 (1.4 X 10(-7) M). The results provide evidence for possible involvement of endogenous prostaglandin-like substances in the maintenance of coronary vascular resistance in the isolated guinea pig heart.", "PMID": 964300}
{"id": "pubmed23n0032_5887", "title": "Isoprenaline and canine cardiac refractory periods.", "content": "Atrioventricular refractory periods and their modifications induced by variable doses of sioprenaline have been investigated in dogs. Premature atrial stimuli (St2) were applied at variable intervals following regular stimuli (St1) which ensured atrial pacing. Atrial (AERP), nodoventricular (NERP) and global (GERP) effective refractory periods, as well as the global functional refractory period (GFRP) were determined before, during and after infusion of the drug. In low doses, isoprenaline did not significantly alter these various parameters. This confirms its lack of dromotropic effect on the healthy heart. In contrast, high doses of the drug significantly reduced the refractory periods, probably by simple correction of the negative dromotropic effects resulting from overstimulation. Also when the infusion was stopped, marked but reversible conduction depression was observed. It would appear that this reflects 'exhaustion' of cell metabolism induced by the drug.", "contents": "Isoprenaline and canine cardiac refractory periods. Atrioventricular refractory periods and their modifications induced by variable doses of sioprenaline have been investigated in dogs. Premature atrial stimuli (St2) were applied at variable intervals following regular stimuli (St1) which ensured atrial pacing. Atrial (AERP), nodoventricular (NERP) and global (GERP) effective refractory periods, as well as the global functional refractory period (GFRP) were determined before, during and after infusion of the drug. In low doses, isoprenaline did not significantly alter these various parameters. This confirms its lack of dromotropic effect on the healthy heart. In contrast, high doses of the drug significantly reduced the refractory periods, probably by simple correction of the negative dromotropic effects resulting from overstimulation. Also when the infusion was stopped, marked but reversible conduction depression was observed. It would appear that this reflects 'exhaustion' of cell metabolism induced by the drug.", "PMID": 964301}
{"id": "pubmed23n0032_5888", "title": "The effects of amiodarone on the sinus node activity of the rabbit heart.", "content": "The action of amiodarone (1.5 X 10(-5) M) on sinus node activity of spontaneously beating isolated right atria of rabbit at 30 degrees C was investigated using a microelectrode technique. The drug significantly increased the action potential duration and decreased the slope of diastolic depolarization, both effects leading to a reduction of the sinus rate. In contrast to beta-blocking agents, amiodarone reduced but did not completely abolish the adrenergic effects on the sinus node activity. It is concluded that the amiodarone-induced bradycarida observed in clinical trials might be due to a direct effect of the drug on the sinus node.", "contents": "The effects of amiodarone on the sinus node activity of the rabbit heart. The action of amiodarone (1.5 X 10(-5) M) on sinus node activity of spontaneously beating isolated right atria of rabbit at 30 degrees C was investigated using a microelectrode technique. The drug significantly increased the action potential duration and decreased the slope of diastolic depolarization, both effects leading to a reduction of the sinus rate. In contrast to beta-blocking agents, amiodarone reduced but did not completely abolish the adrenergic effects on the sinus node activity. It is concluded that the amiodarone-induced bradycarida observed in clinical trials might be due to a direct effect of the drug on the sinus node.", "PMID": 964302}
{"id": "pubmed23n0032_5889", "title": "Effect of probenecid on endogenous and exogenous 3,4-dihydroxyphenylacetic acid and homovanillic acid in the rat brain.", "content": "The two dopamine metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were measured in corpus striatum and hippocampus in rats after an intracerebroventricular injection of DOPAC or HVA. Probenecid 200 mg/kg i.p. increased the concentration of HVA by the same factor in both control animals and animals treated with HVA. Probenecid had no effect on the DOPAC concentration in control animals or in animals treated with DOPAC. After DOPAC intraventricularly some increase in the HVA concentration could be seen. Pargyline 75 mg/kg was given i.m. and the decline of HVA and DOPAC in corpus striatum was compared in animals with or without probenecid treatment (200 mg/kg i.p.). In the control animals DOPAC declined more rapidly than did HVA. In the probenecid-treated animals there was no change in the DOPAC curve while the HVA showed a slower elimination but still with an exponential curve. The conclusion is drawn that there is an active mechanism for the excretion of DOPAC which is not identical with the excretion mechanism for HVA. A considerable part of the dopamine is directly deaminated to DOPAC in vivo and some of this DOPAC is probably O-methylated to HVA.", "contents": "Effect of probenecid on endogenous and exogenous 3,4-dihydroxyphenylacetic acid and homovanillic acid in the rat brain. The two dopamine metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were measured in corpus striatum and hippocampus in rats after an intracerebroventricular injection of DOPAC or HVA. Probenecid 200 mg/kg i.p. increased the concentration of HVA by the same factor in both control animals and animals treated with HVA. Probenecid had no effect on the DOPAC concentration in control animals or in animals treated with DOPAC. After DOPAC intraventricularly some increase in the HVA concentration could be seen. Pargyline 75 mg/kg was given i.m. and the decline of HVA and DOPAC in corpus striatum was compared in animals with or without probenecid treatment (200 mg/kg i.p.). In the control animals DOPAC declined more rapidly than did HVA. In the probenecid-treated animals there was no change in the DOPAC curve while the HVA showed a slower elimination but still with an exponential curve. The conclusion is drawn that there is an active mechanism for the excretion of DOPAC which is not identical with the excretion mechanism for HVA. A considerable part of the dopamine is directly deaminated to DOPAC in vivo and some of this DOPAC is probably O-methylated to HVA.", "PMID": 964303}
{"id": "pubmed23n0032_5890", "title": "Uptake and release of catecholamines in sympathetic nerve fibres in the spleen of the cod, Gadus morhua.", "content": "The effects of drugs known from mammalian experiments to interfere with uptake and release of adrenergic transmitters in the sympathetic nerve terminals have been investigated on perfused spleen and isolated spleen strips from the cod. Cocaine and desipramine inhibit the accumulation of 3H-noradrenaline in the perfused spleen, probably by interfering with uptake mechanisms. Both drugs also shift to the left the dose-response curves for noradrenaline and adrenaline on isolated strips, cocaine being most potent. The maximal contraction force of isolated strips also increases after cocaine when noradrenaline is the agonist, but not when methacholine is used or on chronically denervated strips. This effect of cocaine is therefore not due to a general postsynaptic effect. Tyramine and amphetamine both release stored 3H-noradrenaline from the perfused spleen, and tyramine also contracted the spleen strips. In contrast to the situation in mammals, the dose-response curve for tyramine is not affected by cocaine, and tyramine (up to 10(-3) M) present in the bath does not potentiate the dose-response curve for noradrenaline on isolated strips. Different uptake mechanisms for tyramine and noradrenaline into the nerve terminal are therefore suggested for the cod sympathetic fibres. The alpha-adrenoceptor blocking agent phentolamine produces an increase in overflow of label during nerve stimulation at 5Hz in the perfused spleen preloaded with 3H-noradrenaline. An alpha-adrenoceptor-mediated control of the release of catecholamines, similar to that in mammals, appears to be present in the cod.", "contents": "Uptake and release of catecholamines in sympathetic nerve fibres in the spleen of the cod, Gadus morhua. The effects of drugs known from mammalian experiments to interfere with uptake and release of adrenergic transmitters in the sympathetic nerve terminals have been investigated on perfused spleen and isolated spleen strips from the cod. Cocaine and desipramine inhibit the accumulation of 3H-noradrenaline in the perfused spleen, probably by interfering with uptake mechanisms. Both drugs also shift to the left the dose-response curves for noradrenaline and adrenaline on isolated strips, cocaine being most potent. The maximal contraction force of isolated strips also increases after cocaine when noradrenaline is the agonist, but not when methacholine is used or on chronically denervated strips. This effect of cocaine is therefore not due to a general postsynaptic effect. Tyramine and amphetamine both release stored 3H-noradrenaline from the perfused spleen, and tyramine also contracted the spleen strips. In contrast to the situation in mammals, the dose-response curve for tyramine is not affected by cocaine, and tyramine (up to 10(-3) M) present in the bath does not potentiate the dose-response curve for noradrenaline on isolated strips. Different uptake mechanisms for tyramine and noradrenaline into the nerve terminal are therefore suggested for the cod sympathetic fibres. The alpha-adrenoceptor blocking agent phentolamine produces an increase in overflow of label during nerve stimulation at 5Hz in the perfused spleen preloaded with 3H-noradrenaline. An alpha-adrenoceptor-mediated control of the release of catecholamines, similar to that in mammals, appears to be present in the cod.", "PMID": 964304}
{"id": "pubmed23n0032_5891", "title": "Uptake and release of catecholamines in sympathetic nerve fibers in the spleen of the cod, Gadus morhua.", "content": "The effects of denervation or pretreatment with reserpine or 6-hydroxydopamine on the contractile responses of the cod spleen have been studied. The contraction produced by tyramine is abolished by pretreatment with reserpine or 6-hydroxydopamine and by denervation. The response to tyramine is restored in reserpine-treated strips after exposure to noradrenaline, but this is not the denervated or 6-hydroxydopamine-treated strips. The response to noradrenaline is potentiated by 6-hydroxydopamine treatment or denervation, while neither acute (2 days) nor chronic (8 days) reserpine treatment have any detectable effect. The effects of 6-hydroxydopamine and denervation are probably due to specific supersensitivity caused by the loss of the presynaptic uptake mechanisms on destruction of the nerve terminals, but contribution by a non-specific postsynaptic supersensitivity is not completely excluded. The effect of acetylcholine is also significantly potentiated by pretreatment with 6-hydroxydopamine or denervation, but not by reserpine, while the dose-response curve for carbachol is not affected by the specific cholinesterase inhibitor BW 284 C51, denervation or 6-hydroxydopamine. The degree of potentiation of the acetylcholine curve caused by both denervation and 6-hydroxydopamine treatment is very similar to that produced by BW 284 C51. It is concluded therefore, that the cholinesterase is associated with nerve fibres which are destroyed by denervation and 6-hydroxydopamine. Although the cod spleen receives both cholinergic and adrenergic sympathetic innervation all responses to nerve stimulation of the perfused spleen are abolished after 6-hydroxydopamine treatment. The possibility is suggested that the sympathetic fibres to the cod spleen represent a primitive type of neuron, with both catecholamines and acetylcholine present within the same terminals.", "contents": "Uptake and release of catecholamines in sympathetic nerve fibers in the spleen of the cod, Gadus morhua. The effects of denervation or pretreatment with reserpine or 6-hydroxydopamine on the contractile responses of the cod spleen have been studied. The contraction produced by tyramine is abolished by pretreatment with reserpine or 6-hydroxydopamine and by denervation. The response to tyramine is restored in reserpine-treated strips after exposure to noradrenaline, but this is not the denervated or 6-hydroxydopamine-treated strips. The response to noradrenaline is potentiated by 6-hydroxydopamine treatment or denervation, while neither acute (2 days) nor chronic (8 days) reserpine treatment have any detectable effect. The effects of 6-hydroxydopamine and denervation are probably due to specific supersensitivity caused by the loss of the presynaptic uptake mechanisms on destruction of the nerve terminals, but contribution by a non-specific postsynaptic supersensitivity is not completely excluded. The effect of acetylcholine is also significantly potentiated by pretreatment with 6-hydroxydopamine or denervation, but not by reserpine, while the dose-response curve for carbachol is not affected by the specific cholinesterase inhibitor BW 284 C51, denervation or 6-hydroxydopamine. The degree of potentiation of the acetylcholine curve caused by both denervation and 6-hydroxydopamine treatment is very similar to that produced by BW 284 C51. It is concluded therefore, that the cholinesterase is associated with nerve fibres which are destroyed by denervation and 6-hydroxydopamine. Although the cod spleen receives both cholinergic and adrenergic sympathetic innervation all responses to nerve stimulation of the perfused spleen are abolished after 6-hydroxydopamine treatment. The possibility is suggested that the sympathetic fibres to the cod spleen represent a primitive type of neuron, with both catecholamines and acetylcholine present within the same terminals.", "PMID": 964305}
{"id": "pubmed23n0032_5892", "title": "Enhancement of stereospecific opiate binding to neural membranes by phosphatidyl serine.", "content": "An effort was made to elucidate the possible involvement of phosphatidyl serine in the opiate receptor by examining stereospecific 3H-dihydromorphine binding to neural membrane preparations in the presence of exogenous phosphatidyl serine. The addition of phosphatidyl serine to suspensions of either synaptic membranes or a microsomal fraction significantly enhanced both high and lower affinity binding, the Kd's being 1.0 X 10(-9) and 5.7 X 10(-9) M without lipid and 5.0 X 10(-10) and 3.8 X 10(-9) M with added phosphatidyl serine. Phosphatidyl ethanolamine and lysophosphatidyl ethanolamine had an inhibitory effect on opiate binding. Sulfatides had a slight enhancing effect, while other acidic lipids and lecithin were without any effect. With the use of 3H-dansyl phosphatidyl serine it was established that sufficient exogenous lipid was associated with the membrane to account for the enhancement of opiate binding. The results were discussed from the standpoint of the modification of membrane lipids and their possible significance for the binding of opiates and other ligands. It is suggested that phosphatidyl serine may be an important component of the opiate pharmacophore.", "contents": "Enhancement of stereospecific opiate binding to neural membranes by phosphatidyl serine. An effort was made to elucidate the possible involvement of phosphatidyl serine in the opiate receptor by examining stereospecific 3H-dihydromorphine binding to neural membrane preparations in the presence of exogenous phosphatidyl serine. The addition of phosphatidyl serine to suspensions of either synaptic membranes or a microsomal fraction significantly enhanced both high and lower affinity binding, the Kd's being 1.0 X 10(-9) and 5.7 X 10(-9) M without lipid and 5.0 X 10(-10) and 3.8 X 10(-9) M with added phosphatidyl serine. Phosphatidyl ethanolamine and lysophosphatidyl ethanolamine had an inhibitory effect on opiate binding. Sulfatides had a slight enhancing effect, while other acidic lipids and lecithin were without any effect. With the use of 3H-dansyl phosphatidyl serine it was established that sufficient exogenous lipid was associated with the membrane to account for the enhancement of opiate binding. The results were discussed from the standpoint of the modification of membrane lipids and their possible significance for the binding of opiates and other ligands. It is suggested that phosphatidyl serine may be an important component of the opiate pharmacophore.", "PMID": 964306}
{"id": "pubmed23n0032_5893", "title": "Acute effects of morphine on regional brain levels of acetylcholine in mice and rats.", "content": "Morphine increased levels of acetylcholine in mouse striatum in a dose-dependent manner, the increase occurring at the lowest dose previously found to produce analgesia and coinciding with the time of peak analgesic effect. Naloxone blocked this increase. After repeated injections of high doses of morphine, no effect was seen. The hippocampus was the only other brain region showing an effect, and this after a high dose. In the rat, morphine (30 and 90 mg/kg) increased striatal acetylcholine levels. At these and lower doses (2.5 mg/kg and 10 mg/kg) the ratios in the striatum of levels of acetylcholine to levels of dopamine were significantly increased. Only at the highest dose did morphine increase the levels of dopamine in the striatum and of acetylcholine in the hippocampus. Morphine did not change the levels of dopamine in the striatum and of acetylcholine in the hippocampus. Morphine did not change the levels of norepinephrine in either the hypothalamus or cortex of the rat.", "contents": "Acute effects of morphine on regional brain levels of acetylcholine in mice and rats. Morphine increased levels of acetylcholine in mouse striatum in a dose-dependent manner, the increase occurring at the lowest dose previously found to produce analgesia and coinciding with the time of peak analgesic effect. Naloxone blocked this increase. After repeated injections of high doses of morphine, no effect was seen. The hippocampus was the only other brain region showing an effect, and this after a high dose. In the rat, morphine (30 and 90 mg/kg) increased striatal acetylcholine levels. At these and lower doses (2.5 mg/kg and 10 mg/kg) the ratios in the striatum of levels of acetylcholine to levels of dopamine were significantly increased. Only at the highest dose did morphine increase the levels of dopamine in the striatum and of acetylcholine in the hippocampus. Morphine did not change the levels of dopamine in the striatum and of acetylcholine in the hippocampus. Morphine did not change the levels of norepinephrine in either the hypothalamus or cortex of the rat.", "PMID": 964307}
{"id": "pubmed23n0032_5894", "title": "Specific patterns of neuron arrangement and of synaptic articulation in the medial geniculate body.", "content": "Golgi and electron microscopic analysis of the known cellular layers in concentric shells of the ventro-lateral portion of the medial geniculate body revealed a flat grid of high density neuropil filling the space between the geniculocortical relay cells, forming essentially a single cell layer in each lamina. The \"skeleton\" of this neuropil grid is made up by the interdigitating dendritic tufts of the geniculocortical relay cells, joined together by a rich system of desmosomoid adhesion plaques. The \"holes\" of the \"skeleton\" are filled in by the multilobed dendritic appendages of Golgi type II interneurons and the grape-like terminals of the inferior collicular specific afferents. Additional axon terminals of other sources--terminals of descending corticogenicular fibers, axons of the Golgi type II interneurons and terminals of the initial collaterals of the geniculocortical relay cells--contribute only to a very insignificant fraction of neuropil volume. The Golgi type II interneurons are oriented in perpendicular direction to the cell layers so that they may bridge with their dendrites several successive layers. Although the general expression \"synaptic glomeruli\" used in other relay nuclei for this type of specific synaptic arrangement is hardly applicable to this grid-like neuropil, the essential synaptic articulation pattern of all thalamic relay nuclei is well maintained. The specific inferior collicular afferents are presynaptic to both relay cell dendrites and to the multilobed dendritic appendages of Golgi type II cells, which in turn are presynaptic to the same dendritic regions of the relay cells receiving the bulk of the specific afferents.", "contents": "Specific patterns of neuron arrangement and of synaptic articulation in the medial geniculate body. Golgi and electron microscopic analysis of the known cellular layers in concentric shells of the ventro-lateral portion of the medial geniculate body revealed a flat grid of high density neuropil filling the space between the geniculocortical relay cells, forming essentially a single cell layer in each lamina. The \"skeleton\" of this neuropil grid is made up by the interdigitating dendritic tufts of the geniculocortical relay cells, joined together by a rich system of desmosomoid adhesion plaques. The \"holes\" of the \"skeleton\" are filled in by the multilobed dendritic appendages of Golgi type II interneurons and the grape-like terminals of the inferior collicular specific afferents. Additional axon terminals of other sources--terminals of descending corticogenicular fibers, axons of the Golgi type II interneurons and terminals of the initial collaterals of the geniculocortical relay cells--contribute only to a very insignificant fraction of neuropil volume. The Golgi type II interneurons are oriented in perpendicular direction to the cell layers so that they may bridge with their dendrites several successive layers. Although the general expression \"synaptic glomeruli\" used in other relay nuclei for this type of specific synaptic arrangement is hardly applicable to this grid-like neuropil, the essential synaptic articulation pattern of all thalamic relay nuclei is well maintained. The specific inferior collicular afferents are presynaptic to both relay cell dendrites and to the multilobed dendritic appendages of Golgi type II cells, which in turn are presynaptic to the same dendritic regions of the relay cells receiving the bulk of the specific afferents.", "PMID": 964325}
{"id": "pubmed23n0032_5895", "title": "Types of interneurons and their participation in the neuronal network of the medial geniculate body.", "content": "Three different types of interneurons can be separated in the Golgi picture, and many of their details can be identified under the electron microscope, in the medial geniculate body (MGB) of the cat: (1) typical short axon Golgi II. cells of the thalamic type, (2) somewhat larger Golgi type II cells with medium range axon, and (3) spidery neurogliform short axon cells. The most distinctive features of the two first types (1) and (2) are their irregular drumstick shape appendages, increasing in number as well as in length and irregularity of their stalks towards the periphery of the dendrites. These appendages form the vast majority of synaptic profiles in the aggregations of synaptic neuropil (glomeruli) of the nuclei, and they are both presynaptic and postsynaptic by the usual standards applied for the evaluation of the polarity of synapses. The characteristic beaded dendrites of the (3) neurogliform cell type can be recognised particulary easily in the electron microscopic picture. They are both presynaptic and postsynaptic in structural polarity. All identified process profiles of interneurons contain flattened (F-type) or pleomorphic synaptic vesicles. Membrane contacts, in which the interneurons appear to be presynaptic are either of the symmetric (Gray type II) or of an intermediate type. The membrane contacts of postsynaptic portions of the interneurons are usually of the asymmetric type (Gray type I) and the presynaptic profiles contain round (R-type) vesicles. The larger one have been shown already earlier to be derived from specific sensory (inferior collicular) afferents, while many of the smaller ones could be identified in the present study as being derived from cortico-geniculate descending pathways, arising from the auditory areas. Some of the synaptic contacts of the interneurons are apparently derived from other interneurons, the presynaptic profiles being often equivocal or more likely of axonal origin (all interneurons have clear axons in the Golgi picture). The occurrence of three distinct types of interneurons--probably all of inhibitory nature--the complexity in synaptic arrangement, and more particularly in the dendritic linkage of numerous synaptic sites does not favour such simple explanations as surround inhibition by forward or by backward inhibition, but suggests more sophisticated modes of impulse processing in the MGB.", "contents": "Types of interneurons and their participation in the neuronal network of the medial geniculate body. Three different types of interneurons can be separated in the Golgi picture, and many of their details can be identified under the electron microscope, in the medial geniculate body (MGB) of the cat: (1) typical short axon Golgi II. cells of the thalamic type, (2) somewhat larger Golgi type II cells with medium range axon, and (3) spidery neurogliform short axon cells. The most distinctive features of the two first types (1) and (2) are their irregular drumstick shape appendages, increasing in number as well as in length and irregularity of their stalks towards the periphery of the dendrites. These appendages form the vast majority of synaptic profiles in the aggregations of synaptic neuropil (glomeruli) of the nuclei, and they are both presynaptic and postsynaptic by the usual standards applied for the evaluation of the polarity of synapses. The characteristic beaded dendrites of the (3) neurogliform cell type can be recognised particulary easily in the electron microscopic picture. They are both presynaptic and postsynaptic in structural polarity. All identified process profiles of interneurons contain flattened (F-type) or pleomorphic synaptic vesicles. Membrane contacts, in which the interneurons appear to be presynaptic are either of the symmetric (Gray type II) or of an intermediate type. The membrane contacts of postsynaptic portions of the interneurons are usually of the asymmetric type (Gray type I) and the presynaptic profiles contain round (R-type) vesicles. The larger one have been shown already earlier to be derived from specific sensory (inferior collicular) afferents, while many of the smaller ones could be identified in the present study as being derived from cortico-geniculate descending pathways, arising from the auditory areas. Some of the synaptic contacts of the interneurons are apparently derived from other interneurons, the presynaptic profiles being often equivocal or more likely of axonal origin (all interneurons have clear axons in the Golgi picture). The occurrence of three distinct types of interneurons--probably all of inhibitory nature--the complexity in synaptic arrangement, and more particularly in the dendritic linkage of numerous synaptic sites does not favour such simple explanations as surround inhibition by forward or by backward inhibition, but suggests more sophisticated modes of impulse processing in the MGB.", "PMID": 964326}
{"id": "pubmed23n0032_5896", "title": "Adapting reflexes controlling the human posture.", "content": "Doubt about the role of stretch reflexes in movement and posture control has remained in part because the questions of reflex \"usefulness\" and the postural \"set\" have not been adequately considered in the design of experimental paradigms. The intent of this study was to discover the stabilizing role of stretch reflexes acting upon the ankle musculature while human subjects performed stance tasks requiring several different postural \"sets\". Task specific differences of reflex function were investigated by experiments in which the role of stretch reflexes to stabilize sway doing stance could be altered to be useful, of no use, or inappropriate. Because the system has available a number of alternate inputs to posture (e.g., vestibular and visual), stretch reflex responses were in themselves not necessary to prevent a loss of balance. Nevertheless, 5 out of 12 subjects in this study used long-latency (120 msec) stretch reflexes to help reduce postural sway. Following an unexpected change in the usefulness of stretch reflexes, the 5 subjects progressively altered reflex gain during the succeeding 3-5 trials. Adaptive changes in gain were always in the sense to reduce sway, and therefore could be attenuating or facilitating the reflex response. Comparing subjects using the reflex with those not during so, stretch reflex control resulted in less swaying when the task conditions were unchanging. However, the 5 subjects using reflex controls oftentimes swayed more during the first 3-5 trials after a change, when inappropriate responses were elicited. Four patients with clinically diagnosed cerebellar deficits were studied briefly. Among the stance tasks, their performance was similar to normal in some and significantly poorer in others. Their most significant deficit appeared to be the inability to adapt long-latency reflex gain following changes in the stance task. The study concludes with a discussion of the role of stretch reflexes within a hierarchy of controls ranging from muscle stiffness up to centrally initiated responses.", "contents": "Adapting reflexes controlling the human posture. Doubt about the role of stretch reflexes in movement and posture control has remained in part because the questions of reflex \"usefulness\" and the postural \"set\" have not been adequately considered in the design of experimental paradigms. The intent of this study was to discover the stabilizing role of stretch reflexes acting upon the ankle musculature while human subjects performed stance tasks requiring several different postural \"sets\". Task specific differences of reflex function were investigated by experiments in which the role of stretch reflexes to stabilize sway doing stance could be altered to be useful, of no use, or inappropriate. Because the system has available a number of alternate inputs to posture (e.g., vestibular and visual), stretch reflex responses were in themselves not necessary to prevent a loss of balance. Nevertheless, 5 out of 12 subjects in this study used long-latency (120 msec) stretch reflexes to help reduce postural sway. Following an unexpected change in the usefulness of stretch reflexes, the 5 subjects progressively altered reflex gain during the succeeding 3-5 trials. Adaptive changes in gain were always in the sense to reduce sway, and therefore could be attenuating or facilitating the reflex response. Comparing subjects using the reflex with those not during so, stretch reflex control resulted in less swaying when the task conditions were unchanging. However, the 5 subjects using reflex controls oftentimes swayed more during the first 3-5 trials after a change, when inappropriate responses were elicited. Four patients with clinically diagnosed cerebellar deficits were studied briefly. Among the stance tasks, their performance was similar to normal in some and significantly poorer in others. Their most significant deficit appeared to be the inability to adapt long-latency reflex gain following changes in the stance task. The study concludes with a discussion of the role of stretch reflexes within a hierarchy of controls ranging from muscle stiffness up to centrally initiated responses.", "PMID": 964327}
{"id": "pubmed23n0032_5897", "title": "Effect of peripheral anatomy on the fine structure and histochemistry of the Rolando substance: degenerative atrophy of central processes of pseudounipolar cells.", "content": "Disappearance of fluorid-resistant acid phosphatase activity from the ipsilateral Rolando substance after transection of the peripheral nerve, is shown to be due to the cessation of enzyme supply from dorsal root ganglion cells to their central terminals. This is accompanied by (or ensues in consequence of) a fine structural derangement of these terminals (\"degenerative atrophy\"). Fine structural alterations of axon terminals undergoing degenerative atrophy, though similar to some extent to those seen during early phases of a Wallerian degeneration, are markedly different. Also myelinated nerve fibers, both in the dorsal horn and in dorsal columns, are affected by degenerative atrophy. This important, new trophical feature of sensory ganglion cells suggests a delicate metabolic balance between peripheral and central axonal branches of bipolar (pseudounipolar) cells. Degenerative atrophy raises serious implications in evaluating hodological experiments based upon Wallerian degeneration and offers new perspectives for theoretical and clinical neurology.", "contents": "Effect of peripheral anatomy on the fine structure and histochemistry of the Rolando substance: degenerative atrophy of central processes of pseudounipolar cells. Disappearance of fluorid-resistant acid phosphatase activity from the ipsilateral Rolando substance after transection of the peripheral nerve, is shown to be due to the cessation of enzyme supply from dorsal root ganglion cells to their central terminals. This is accompanied by (or ensues in consequence of) a fine structural derangement of these terminals (\"degenerative atrophy\"). Fine structural alterations of axon terminals undergoing degenerative atrophy, though similar to some extent to those seen during early phases of a Wallerian degeneration, are markedly different. Also myelinated nerve fibers, both in the dorsal horn and in dorsal columns, are affected by degenerative atrophy. This important, new trophical feature of sensory ganglion cells suggests a delicate metabolic balance between peripheral and central axonal branches of bipolar (pseudounipolar) cells. Degenerative atrophy raises serious implications in evaluating hodological experiments based upon Wallerian degeneration and offers new perspectives for theoretical and clinical neurology.", "PMID": 964328}
{"id": "pubmed23n0032_5898", "title": "Inhibitory interaction between the vestibulo-ocular reflexes arising from semicircular canals of rabbits.", "content": "In anesthetized albino rabbits, electric pulse stimulation was applied to ampullary branches of the vestibular nerve. Reflex discharges evoked from a canal in an extraocular muscle were depressed very effectively by conditioning stimulation at a certain other canal. The present systematic survey revealed that this reflex depression occurred specifically in 3 combinations of conditioning and testing canals; 1. anterior and posterior canals of the same side; 2. anterior and posterior canals of the opposite sides; and 3. horizontal canals of the two sides. Occurrence of postsynaptic inhibition in oculomotor neurons, on the other hand, was indicated by appearance of slow muscle potentials in extraocular muscles. It was confirmed that this motoneuronal inhibition did not contribute to the reflex depression in the above combination (1). Even in combinations (2) and (3), the accompanying motoneuronal inhibition was eliminated by adjusting intensities of canal stimuli or by severing its pathway in the medulla, or it was discriminated from the reflex depression by their different latencies and time courses. Hence, it was concluded that the reflex depression was attributable, at least largely, to non-motoneuronal inhibition, presumably postsynaptic inhibition at relay neurons for vestibulo-ocular reflexes. Slow muscle potentials evoked from a canal were also used as testing responses, but their depression could not be detected after conditioning at other canals.", "contents": "Inhibitory interaction between the vestibulo-ocular reflexes arising from semicircular canals of rabbits. In anesthetized albino rabbits, electric pulse stimulation was applied to ampullary branches of the vestibular nerve. Reflex discharges evoked from a canal in an extraocular muscle were depressed very effectively by conditioning stimulation at a certain other canal. The present systematic survey revealed that this reflex depression occurred specifically in 3 combinations of conditioning and testing canals; 1. anterior and posterior canals of the same side; 2. anterior and posterior canals of the opposite sides; and 3. horizontal canals of the two sides. Occurrence of postsynaptic inhibition in oculomotor neurons, on the other hand, was indicated by appearance of slow muscle potentials in extraocular muscles. It was confirmed that this motoneuronal inhibition did not contribute to the reflex depression in the above combination (1). Even in combinations (2) and (3), the accompanying motoneuronal inhibition was eliminated by adjusting intensities of canal stimuli or by severing its pathway in the medulla, or it was discriminated from the reflex depression by their different latencies and time courses. Hence, it was concluded that the reflex depression was attributable, at least largely, to non-motoneuronal inhibition, presumably postsynaptic inhibition at relay neurons for vestibulo-ocular reflexes. Slow muscle potentials evoked from a canal were also used as testing responses, but their depression could not be detected after conditioning at other canals.", "PMID": 964329}
{"id": "pubmed23n0032_5899", "title": "The social consequences of teenage parenthood.", "content": "The adolescent mothers consistently experienced great difficulty in realizing their life plans, when compared with their classmates who did not become pregnant premaritally in their early teens. Marital instability, school disruption, economic problems, and difficulty in family size regulation and child-rearing were some of the complications brought on by their premature, unscheduled childbearing.", "contents": "The social consequences of teenage parenthood. The adolescent mothers consistently experienced great difficulty in realizing their life plans, when compared with their classmates who did not become pregnant premaritally in their early teens. Marital instability, school disruption, economic problems, and difficulty in family size regulation and child-rearing were some of the complications brought on by their premature, unscheduled childbearing.", "PMID": 964349}
{"id": "pubmed23n0032_5900", "title": "Fertility control services for adolescents: access and utilization.", "content": "More than one million teenagers get pregnant each year; six in 10 pregnancies end in live births--the rest in induced or spontaneous abortions. More than one-third of the births are out of wedlock. Yet, two-thirds of teenage pregnancies and half of the births are not intended. Some two million teenagers at risk of unintended pregnancy are now using effective birth control methods; but two millions are still denied them.", "contents": "Fertility control services for adolescents: access and utilization. More than one million teenagers get pregnant each year; six in 10 pregnancies end in live births--the rest in induced or spontaneous abortions. More than one-third of the births are out of wedlock. Yet, two-thirds of teenage pregnancies and half of the births are not intended. Some two million teenagers at risk of unintended pregnancy are now using effective birth control methods; but two millions are still denied them.", "PMID": 964350}
{"id": "pubmed23n0032_5901", "title": "Teenagers: fertility control behavior and attitudes before and after abortion, childbearing or negative pregnancy test.", "content": "Following abortion or delivery, teenagers' knowledge and use of effective contraception improved markedly; but those who had negative pregnancy tests continued to take risks--and to get pregnant--subsequently. Most sexually active teenagers were poorly educated on the facts of reproduction, and began to have intercourse before seeking contraception.", "contents": "Teenagers: fertility control behavior and attitudes before and after abortion, childbearing or negative pregnancy test. Following abortion or delivery, teenagers' knowledge and use of effective contraception improved markedly; but those who had negative pregnancy tests continued to take risks--and to get pregnant--subsequently. Most sexually active teenagers were poorly educated on the facts of reproduction, and began to have intercourse before seeking contraception.", "PMID": 964352}
{"id": "pubmed23n0032_5902", "title": "Circadian rhythms and photoperiodic time measurement in mammals.", "content": "Many mammalian species display seasonal breeding patterns correlated with annual cycles of change in the physiology and morphology of the reproductive system. Such annual reproductive cycles are often photoperiodically controlled (i.e, the annual change in day length determines when reproductive activity begins and when it ends within the annual cycle). Photoperiodic control of seasonally appropriate changes in reproductive activity is dependent on an endogenous time measuring process. Among mammals the physiological basis of photoperiodic time measurement has been studied most extensively in the golden hamster. Studies with this species indicate that photoperiodic time measurement is executed by the circadian system. The time measuring process depends on a circadian oscillation of responsiveness to light with properties similar to those of the hypothetical rhythm originally proposed by B\u00fcnning to explain photoperiodic phenomena in plants. The available evidence strongly suggests the participation of the pineal gland and the suprachiasmatic nucleus (SCN) in the photoperiodic regulation of mammalian reproductive cycles. However, little is known regarding concrete physiological mechanisms, and the extent to which the SCN and the pineal gland may participate in the time measuring process per se remains to be determined.", "contents": "Circadian rhythms and photoperiodic time measurement in mammals. Many mammalian species display seasonal breeding patterns correlated with annual cycles of change in the physiology and morphology of the reproductive system. Such annual reproductive cycles are often photoperiodically controlled (i.e, the annual change in day length determines when reproductive activity begins and when it ends within the annual cycle). Photoperiodic control of seasonally appropriate changes in reproductive activity is dependent on an endogenous time measuring process. Among mammals the physiological basis of photoperiodic time measurement has been studied most extensively in the golden hamster. Studies with this species indicate that photoperiodic time measurement is executed by the circadian system. The time measuring process depends on a circadian oscillation of responsiveness to light with properties similar to those of the hypothetical rhythm originally proposed by B\u00fcnning to explain photoperiodic phenomena in plants. The available evidence strongly suggests the participation of the pineal gland and the suprachiasmatic nucleus (SCN) in the photoperiodic regulation of mammalian reproductive cycles. However, little is known regarding concrete physiological mechanisms, and the extent to which the SCN and the pineal gland may participate in the time measuring process per se remains to be determined.", "PMID": 964387}
{"id": "pubmed23n0032_5903", "title": "Renal prostaglandins in relation to sodium regulation and hypertension.", "content": "The renal inner medulla is very rich in prostaglandins as well as in the enzymes that biosynthesize prostaglandins. When given intravenously, prostaglandin E or A will cause a natriuresis. Using a quick-freeze method one can measure the in vivo concentration of prostaglandins in the kidney. After a high NaCl intake for 2 weeks, the prostaglandin E2 (PGE2) concentration in rat kidneys decreases 40% below control. After 2 weeks of a low NaCl diet, the intrarenal PGE2 concentration approximately doubles. Blockers of prostaglandin synthesis have recently been shown to cause a natriuresis. These observations are compatible with the possibility that intrarenal PGE2 acts locally as an antinatriuretic hormone. Intrarenal prostaglandins may also have a role in certain forms of hypertension. The interstitial cells of the renal papilla appear to be able to secret antihypertensive humoral substances and can also synthesize prostaglandins. They have diminished numbers of cytoplasmic lipid granules in several forms of experimental hypertension. Moreover, using the quick-freeze method, the kidneys of \"post-salt\" hypertensive rats have a 36% lower intrarenal PGE2 level than is present in \"post-salt\" normotensive controls. Moreover, the kidneys from Kyoto spontaneously hypertensive rats have almost a three times greater intrarenal PGF2alpha concentration from Kyoto normotensive controls. The abnormal prostaglandin concentrations in these two types of hypertension may constitute key integral features of the control mechanisms through which the kidney brings about high blood pressure.", "contents": "Renal prostaglandins in relation to sodium regulation and hypertension. The renal inner medulla is very rich in prostaglandins as well as in the enzymes that biosynthesize prostaglandins. When given intravenously, prostaglandin E or A will cause a natriuresis. Using a quick-freeze method one can measure the in vivo concentration of prostaglandins in the kidney. After a high NaCl intake for 2 weeks, the prostaglandin E2 (PGE2) concentration in rat kidneys decreases 40% below control. After 2 weeks of a low NaCl diet, the intrarenal PGE2 concentration approximately doubles. Blockers of prostaglandin synthesis have recently been shown to cause a natriuresis. These observations are compatible with the possibility that intrarenal PGE2 acts locally as an antinatriuretic hormone. Intrarenal prostaglandins may also have a role in certain forms of hypertension. The interstitial cells of the renal papilla appear to be able to secret antihypertensive humoral substances and can also synthesize prostaglandins. They have diminished numbers of cytoplasmic lipid granules in several forms of experimental hypertension. Moreover, using the quick-freeze method, the kidneys of \"post-salt\" hypertensive rats have a 36% lower intrarenal PGE2 level than is present in \"post-salt\" normotensive controls. Moreover, the kidneys from Kyoto spontaneously hypertensive rats have almost a three times greater intrarenal PGF2alpha concentration from Kyoto normotensive controls. The abnormal prostaglandin concentrations in these two types of hypertension may constitute key integral features of the control mechanisms through which the kidney brings about high blood pressure.", "PMID": 964389}
{"id": "pubmed23n0032_5904", "title": "An outbreak of methylmercury poisoning due to consumption of contaminated grain.", "content": "An outbreak of methylmercury poisoning took place in the fall and winter of 1971-72 in Iraq. Six thousand five hundred and thirty cases were admitted to hospitals throughout the country and 459 died in hospital. The outbreak was the result of eating homemade bread prepared from wheat treated with a methylmercury fungicide. The wheat was intended for planting purposes only. Signs and symptoms of poisonings in adults indicate that the major site of action of this form of mercury is the central nervous system. Severe brain damage also resulted from prenatal exposure when the mother ingested large amounts of the contaminated bread. The frequency of signs and symptoms in an exposed population was found to be related to the estimated maximum blood levels, i.e., the concentration in blood at the end of exposure. A small percentage of the population exhibited a significant increase in complaints of paresthesia at maximum blood levels in the range of 240 to 480 ng Hg/ml. At higher blood levels a greater proportion of the population complained of paresthesia and other signs and symptoms became apparent.", "contents": "An outbreak of methylmercury poisoning due to consumption of contaminated grain. An outbreak of methylmercury poisoning took place in the fall and winter of 1971-72 in Iraq. Six thousand five hundred and thirty cases were admitted to hospitals throughout the country and 459 died in hospital. The outbreak was the result of eating homemade bread prepared from wheat treated with a methylmercury fungicide. The wheat was intended for planting purposes only. Signs and symptoms of poisonings in adults indicate that the major site of action of this form of mercury is the central nervous system. Severe brain damage also resulted from prenatal exposure when the mother ingested large amounts of the contaminated bread. The frequency of signs and symptoms in an exposed population was found to be related to the estimated maximum blood levels, i.e., the concentration in blood at the end of exposure. A small percentage of the population exhibited a significant increase in complaints of paresthesia at maximum blood levels in the range of 240 to 480 ng Hg/ml. At higher blood levels a greater proportion of the population complained of paresthesia and other signs and symptoms became apparent.", "PMID": 964390}
{"id": "pubmed23n0032_5905", "title": "Evaluation of intramuscular 15(s)-15-methyl prostaglandin F2 alpha tromethamine salt for induction of abortion, medications to attenuate side effects, and intracervical laminaria tents.", "content": "Sixty gravidas 8 to 20 menstrual weeks' gestation were studied to evaluate (1) the efficacy of intramuscularly administered 15(S)-15-methyl prostaglandin F2alpha tromethamine (15(S)-Me-PGF2alpha) as an abortifacient; (2) the effectiveness of prochlorperazine and Lomotil for attenuation of vomiting and diarrhea; and (3) the practicability of augmenting this prostaglandin dose schedule with intracervical laminaria tents. Group I subjects received 250 mug of 15(S)-Me-PGF2alpha intramuscularly every 2 hours for the initial 24 hours and 500 mug for the next 24-hour period. Group II received the same dose schedule of prostaglandin and prearranged doses of prochlorperazine and Lomotil. Group III received the same dose schedule of prostaglandin after intracervical laminaria tents had been inserted, and prochlorperazine and Lomotil were administered by the prearranged dose schedule. It appears that (1) the 15(S)-Me-PGF2alpha was effective in inducing abortion; (2) a significant decrease in body temperature occurred; (3) the abortifacient effectiveness of this prostaglandin dose schedule was not altered by the regimen of prochlorperazine and Lomotil; (4) only diarrhea was significantly attenuated with the regimen of prochlorperazine and Lomotil; and (5) laminaria augmentation was not useful.", "contents": "Evaluation of intramuscular 15(s)-15-methyl prostaglandin F2 alpha tromethamine salt for induction of abortion, medications to attenuate side effects, and intracervical laminaria tents. Sixty gravidas 8 to 20 menstrual weeks' gestation were studied to evaluate (1) the efficacy of intramuscularly administered 15(S)-15-methyl prostaglandin F2alpha tromethamine (15(S)-Me-PGF2alpha) as an abortifacient; (2) the effectiveness of prochlorperazine and Lomotil for attenuation of vomiting and diarrhea; and (3) the practicability of augmenting this prostaglandin dose schedule with intracervical laminaria tents. Group I subjects received 250 mug of 15(S)-Me-PGF2alpha intramuscularly every 2 hours for the initial 24 hours and 500 mug for the next 24-hour period. Group II received the same dose schedule of prostaglandin and prearranged doses of prochlorperazine and Lomotil. Group III received the same dose schedule of prostaglandin after intracervical laminaria tents had been inserted, and prochlorperazine and Lomotil were administered by the prearranged dose schedule. It appears that (1) the 15(S)-Me-PGF2alpha was effective in inducing abortion; (2) a significant decrease in body temperature occurred; (3) the abortifacient effectiveness of this prostaglandin dose schedule was not altered by the regimen of prochlorperazine and Lomotil; (4) only diarrhea was significantly attenuated with the regimen of prochlorperazine and Lomotil; and (5) laminaria augmentation was not useful.", "PMID": 964392}
{"id": "pubmed23n0032_5906", "title": "Intrauterine extra-amniotic 15(s)-15-methyl prostaglandin f2 alpha for induction of early midtrimester abortion.", "content": "Twenty-six patients 13 to 15 weeks pregnant received extraovular 15(S)-15-methyl prostaglandin F2 alpha in a dosage of 1 mg every 18 to 24 hours for three doses. Twenty-five (96%) of the patients aborted; fifteen patients aborted incompletely. The mean induction-to-abortion time was 15.8 hours. A decrease in plasma progesterone levels could not be consistently correlated with abortifacient activity, and there was no statistically significant relationship between abortion time and parity or gestational age. One patient failed to abort after 36 hours of therapy and was aborted by curettage. She was the only patient who developed a postabortion uterine infection. Results with the extraovular technique appear to be similar to those with the intra-amniotic and the intramuscular prostaglandin techniques. Therefore, extraovular administration may be useful for inducing abortion in patients who are in the early midtrimester of pregnancy.", "contents": "Intrauterine extra-amniotic 15(s)-15-methyl prostaglandin f2 alpha for induction of early midtrimester abortion. Twenty-six patients 13 to 15 weeks pregnant received extraovular 15(S)-15-methyl prostaglandin F2 alpha in a dosage of 1 mg every 18 to 24 hours for three doses. Twenty-five (96%) of the patients aborted; fifteen patients aborted incompletely. The mean induction-to-abortion time was 15.8 hours. A decrease in plasma progesterone levels could not be consistently correlated with abortifacient activity, and there was no statistically significant relationship between abortion time and parity or gestational age. One patient failed to abort after 36 hours of therapy and was aborted by curettage. She was the only patient who developed a postabortion uterine infection. Results with the extraovular technique appear to be similar to those with the intra-amniotic and the intramuscular prostaglandin techniques. Therefore, extraovular administration may be useful for inducing abortion in patients who are in the early midtrimester of pregnancy.", "PMID": 964393}
{"id": "pubmed23n0032_5907", "title": "The detection of ovulation by intravaginal telemetry.", "content": "Following ovulatory control cycles in four celibate, healthy women, 27 to 34 years of age, basal body temperatures (BBT) were obtained in three cycles, utilizing a transistorized transvaginal telemeter. The electronic signals were collected on an automatically activated cassette receiver and later translated into temperature readings. The results were compared with values obtained simultaneously with a standard metabolic oral thermometer. The vaginal temperatures were consistently lower than the oral readings and offered a more sensitive and reproducible method of obtaining BBT. However, this system is very complex, requiring well-informed, highly motivated patients, and should be viewed currently as an investigative tool.", "contents": "The detection of ovulation by intravaginal telemetry. Following ovulatory control cycles in four celibate, healthy women, 27 to 34 years of age, basal body temperatures (BBT) were obtained in three cycles, utilizing a transistorized transvaginal telemeter. The electronic signals were collected on an automatically activated cassette receiver and later translated into temperature readings. The results were compared with values obtained simultaneously with a standard metabolic oral thermometer. The vaginal temperatures were consistently lower than the oral readings and offered a more sensitive and reproducible method of obtaining BBT. However, this system is very complex, requiring well-informed, highly motivated patients, and should be viewed currently as an investigative tool.", "PMID": 964394}
{"id": "pubmed23n0032_5908", "title": "Sutureless vasectomy, an improved technique: 1300 cases performed without failure.", "content": "Thirteen hundred bilateral, partial vasectomies have been performed for sterilization, without a failure. The procedure utilizes (1) a small, single incision; (2) no sutures; (3) cautery of each vas deferens lumen with a hand-held, battery-operated, disposable, hot-wire cautery; and (4) sealing of the proximal vas deferens in its sheath with a single tantalum clip. No spermatic granulomas were noted using this technique.", "contents": "Sutureless vasectomy, an improved technique: 1300 cases performed without failure. Thirteen hundred bilateral, partial vasectomies have been performed for sterilization, without a failure. The procedure utilizes (1) a small, single incision; (2) no sutures; (3) cautery of each vas deferens lumen with a hand-held, battery-operated, disposable, hot-wire cautery; and (4) sealing of the proximal vas deferens in its sheath with a single tantalum clip. No spermatic granulomas were noted using this technique.", "PMID": 964395}
{"id": "pubmed23n0032_5909", "title": "Varicocelectomy in the subfertile male: a ten-year experience with 295 cases.", "content": "During a 10-year period 295 varicocelectomies were performed in subfertile males. Results of follow-up studies indicate the following: (1) Semen quality improved in a meaningful manner in 58% of men following varicocelectomy. The improvement, except at times for motility, was often only modest. The semen improved in 46% of men with a preoperative average count of less than 10 million/ml and in 70% whose count was 10 million/ml or more. (2) The stress pattern of sperm morphology was seen prior to surgery in 93% of the men. (3) The pregnancy rate for the entire series was about 41%. Men with a preoperative count of 10 million/ml or more achieved a 48% conception rate and those with a count of less than 10 million/ml succeeded 35% of the time. Only 7% of the pregnancies occurred without a concomitant improvement in the semen quality. (4) Men with moderate- and large-sized left varicoceles fared better than those with small-sized varicoceles. (5) Varicocelectomy, when varicocele and poor semen quality coexist, is a justified procedure in the treatment of the infertile couple.", "contents": "Varicocelectomy in the subfertile male: a ten-year experience with 295 cases. During a 10-year period 295 varicocelectomies were performed in subfertile males. Results of follow-up studies indicate the following: (1) Semen quality improved in a meaningful manner in 58% of men following varicocelectomy. The improvement, except at times for motility, was often only modest. The semen improved in 46% of men with a preoperative average count of less than 10 million/ml and in 70% whose count was 10 million/ml or more. (2) The stress pattern of sperm morphology was seen prior to surgery in 93% of the men. (3) The pregnancy rate for the entire series was about 41%. Men with a preoperative count of 10 million/ml or more achieved a 48% conception rate and those with a count of less than 10 million/ml succeeded 35% of the time. Only 7% of the pregnancies occurred without a concomitant improvement in the semen quality. (4) Men with moderate- and large-sized left varicoceles fared better than those with small-sized varicoceles. (5) Varicocelectomy, when varicocele and poor semen quality coexist, is a justified procedure in the treatment of the infertile couple.", "PMID": 964396}
{"id": "pubmed23n0032_5910", "title": "Linear and quantitative migration of stored sperm through cervical mucus during the periovular period.", "content": "The ability of stored spermatozoa to penetrate fresh samples of cervical mucus obtained from 23 patients during 47 menstrual cycles was studied, utilizing an in vitro test. The determination of daily hormonal and gonadotropin values allowed an accurate realationship to be established between the results of sperm-mucus interaction and the environmental milieu. Cervical mucus was found to be effectively penetrable only on day -1 and day 0 when considered with respect to the plasma luteinizing hormone surge. Correlation was apparent between the ability of sperm to penetrate and the favorable clinical parameters of cervical assessment.", "contents": "Linear and quantitative migration of stored sperm through cervical mucus during the periovular period. The ability of stored spermatozoa to penetrate fresh samples of cervical mucus obtained from 23 patients during 47 menstrual cycles was studied, utilizing an in vitro test. The determination of daily hormonal and gonadotropin values allowed an accurate realationship to be established between the results of sperm-mucus interaction and the environmental milieu. Cervical mucus was found to be effectively penetrable only on day -1 and day 0 when considered with respect to the plasma luteinizing hormone surge. Correlation was apparent between the ability of sperm to penetrate and the favorable clinical parameters of cervical assessment.", "PMID": 964397}
{"id": "pubmed23n0032_5911", "title": "Serum luteinizing hormone, follicle-stimulating hormone, and testosterone responses to gonadotropin-releasing factor in males with varicoceles.", "content": "Five infertile males, ages 25 to 35, with oligospermia and varicocele had following gonadotropin-releasing hormone (LRF) infusion a rise of serum follicle-stimulating hormone, luteinizing hormone, and testosterone levels which was not different from that of normal fertile males. The response of these hormones to LRF infusion was unaltered by spermatic vein ligation, but a significant elevation of the sperm count occurred. Thus, improvement in sperm count following spermatic vein ligation is not mediated via changes in peripheral gonadotropin or testosterone concentrations.", "contents": "Serum luteinizing hormone, follicle-stimulating hormone, and testosterone responses to gonadotropin-releasing factor in males with varicoceles. Five infertile males, ages 25 to 35, with oligospermia and varicocele had following gonadotropin-releasing hormone (LRF) infusion a rise of serum follicle-stimulating hormone, luteinizing hormone, and testosterone levels which was not different from that of normal fertile males. The response of these hormones to LRF infusion was unaltered by spermatic vein ligation, but a significant elevation of the sperm count occurred. Thus, improvement in sperm count following spermatic vein ligation is not mediated via changes in peripheral gonadotropin or testosterone concentrations.", "PMID": 964398}
{"id": "pubmed23n0032_5912", "title": "Detection of human chorionic gonadotropin in blood of regularly bleeding women using copper intrauterine contraceptive devices.", "content": "Highly sensitive and specific radioreceptorassay and radioimmunoassay of human chorionic gonadtropin (hCG) have been used in the detection of hCG in random serum samples during the luteal phase of the menstrual cycles of 200 women and in daily serum samples obtained a few days prior to expected ovulation through the luteal phase in 3 women with regular bleeding patterns and using a copper intrauterine device (IUD). Twelve to nineteen per cent of IUD users showed hCG in serum during the luteal phase, indicating that the presence of the IUD, while permitting fertilization, probably causes interference through degeneration of the blastocyst and consequent lack of implantation.", "contents": "Detection of human chorionic gonadotropin in blood of regularly bleeding women using copper intrauterine contraceptive devices. Highly sensitive and specific radioreceptorassay and radioimmunoassay of human chorionic gonadtropin (hCG) have been used in the detection of hCG in random serum samples during the luteal phase of the menstrual cycles of 200 women and in daily serum samples obtained a few days prior to expected ovulation through the luteal phase in 3 women with regular bleeding patterns and using a copper intrauterine device (IUD). Twelve to nineteen per cent of IUD users showed hCG in serum during the luteal phase, indicating that the presence of the IUD, while permitting fertilization, probably causes interference through degeneration of the blastocyst and consequent lack of implantation.", "PMID": 964399}
{"id": "pubmed23n0032_5913", "title": "Embryotoxicity of leukocyte extracts and its relationship to intrauterine contraception in humans.", "content": "Twelve human uteri containing intrauterine contraceptive devices (IUDs) and ten uteri without IUDs were obtained at hysterectomy. Samples of fluid were collected from the uterine lumina by absorbing the fluid in small pieces of lens paper. In the samples of luminal fluid we measured the concentration of beta-galactosidase, an enzyme which is present in human neutrophilic leukocytes and whose concentration in luminal fluid should correlate with the local inflammatory response to the intrauterine foreign body. In the samples of fluid from IUD-bearing uteri, the concentration of beta-galactosidase was significantly (P less than 0.0005) greater than that in luminal fluid from control uteri, the averages of the two groups differing by 3.8 units. To determine whether a foreign-body response of this magnitude could have any effect on preimplantation embryos, we cultured mouse embryos from day 4 to day 7 of development in culture media to which extracts of human leukocytes were added. All mouse embryos were killed when the culture media contained enough leukocyte extract to give beta-galactosidase concentrations of 0.5 unit or higher. Thus mouse embryos were killed by leukocyte extracts whose beta-galactosidase concentrations were actually less than the concentration of this marker enzyme measured in IUD uterine fluid. This comparison indicates that the luminal fluid in IUD-bearing uteri contains leukocyte break-down products in sufficient concentration to be lethal for preimplantation embryos.", "contents": "Embryotoxicity of leukocyte extracts and its relationship to intrauterine contraception in humans. Twelve human uteri containing intrauterine contraceptive devices (IUDs) and ten uteri without IUDs were obtained at hysterectomy. Samples of fluid were collected from the uterine lumina by absorbing the fluid in small pieces of lens paper. In the samples of luminal fluid we measured the concentration of beta-galactosidase, an enzyme which is present in human neutrophilic leukocytes and whose concentration in luminal fluid should correlate with the local inflammatory response to the intrauterine foreign body. In the samples of fluid from IUD-bearing uteri, the concentration of beta-galactosidase was significantly (P less than 0.0005) greater than that in luminal fluid from control uteri, the averages of the two groups differing by 3.8 units. To determine whether a foreign-body response of this magnitude could have any effect on preimplantation embryos, we cultured mouse embryos from day 4 to day 7 of development in culture media to which extracts of human leukocytes were added. All mouse embryos were killed when the culture media contained enough leukocyte extract to give beta-galactosidase concentrations of 0.5 unit or higher. Thus mouse embryos were killed by leukocyte extracts whose beta-galactosidase concentrations were actually less than the concentration of this marker enzyme measured in IUD uterine fluid. This comparison indicates that the luminal fluid in IUD-bearing uteri contains leukocyte break-down products in sufficient concentration to be lethal for preimplantation embryos.", "PMID": 964400}
{"id": "pubmed23n0032_5914", "title": "Surface changes of the rat embryo before implantation.", "content": "Study of the rat embryo surface under the scanning electron microscope shows the superficial structure of the pellucid membrane as a perforated network cover which does not change throughout the preimplantation period (1 to 41/2 days). Dissolution of the pellucid membrane by brief ATP treatment reveals a zygotic surface which changes from day to day. The number and length of microvilli increase with development. At the early blastula stage there is a great heterogeneity of microvilli of different size and shape as well as large membranous ruffles mainly located at one pole of the embryo. The significance of these structures may relate to the changing metabolic requirements of the developing embryo and to the invasive properties of the trophoblast.", "contents": "Surface changes of the rat embryo before implantation. Study of the rat embryo surface under the scanning electron microscope shows the superficial structure of the pellucid membrane as a perforated network cover which does not change throughout the preimplantation period (1 to 41/2 days). Dissolution of the pellucid membrane by brief ATP treatment reveals a zygotic surface which changes from day to day. The number and length of microvilli increase with development. At the early blastula stage there is a great heterogeneity of microvilli of different size and shape as well as large membranous ruffles mainly located at one pole of the embryo. The significance of these structures may relate to the changing metabolic requirements of the developing embryo and to the invasive properties of the trophoblast.", "PMID": 964401}
{"id": "pubmed23n0032_5915", "title": "Measurement of androstanediol in plasma in a radioimmunoassay using celite column chromatography.", "content": "A rapid and relatively simple, but specific, radioimmunoassay for the potent androgen, androstanediol (3 alpha-diol), is described. Despite the availability of a nonspecific C-19 androgen antibody requiring a 17 beta-hydroxy group, androstanediol can be measured in plasma by prior purification of a plasma solvent extract using a Celite microcolumn. Values obtained do not differ from those previously reported using more complicated chromatographic techniques.", "contents": "Measurement of androstanediol in plasma in a radioimmunoassay using celite column chromatography. A rapid and relatively simple, but specific, radioimmunoassay for the potent androgen, androstanediol (3 alpha-diol), is described. Despite the availability of a nonspecific C-19 androgen antibody requiring a 17 beta-hydroxy group, androstanediol can be measured in plasma by prior purification of a plasma solvent extract using a Celite microcolumn. Values obtained do not differ from those previously reported using more complicated chromatographic techniques.", "PMID": 964402}
{"id": "pubmed23n0032_5916", "title": "Preliminary results of the endometrial and ovarian response to luteal phase norgestrel.", "content": "Luteal phase administration of dl-norgestrel, in doses of 10 mg to two groups of women with ovulatory cycles, did not significantly affect the length of the menstrual cycles, the duration of menses, or the endometrium whether given on a 5-consecutive day (group A) or 5-alternate day schedule (group B). Serum progesterone levels decreased in 9 of 11 patients in group A from a pretreatment level of 1431 ng/100 ml to 568 ng/100 ml and then to 419 ng/100 ml for the first and third treatment cycles, respectively; corresponding values for group B were 1149 ng/100 ml, 237 ng/100 ml, and less than 100 ng/100 ml, respectively. No adverse reactions were noted in any of the subjects; Papanicolaou smears remained class I, blood chemistry values showed no significant changes from pretreatment values, and no pregnancies occurred during treatment.", "contents": "Preliminary results of the endometrial and ovarian response to luteal phase norgestrel. Luteal phase administration of dl-norgestrel, in doses of 10 mg to two groups of women with ovulatory cycles, did not significantly affect the length of the menstrual cycles, the duration of menses, or the endometrium whether given on a 5-consecutive day (group A) or 5-alternate day schedule (group B). Serum progesterone levels decreased in 9 of 11 patients in group A from a pretreatment level of 1431 ng/100 ml to 568 ng/100 ml and then to 419 ng/100 ml for the first and third treatment cycles, respectively; corresponding values for group B were 1149 ng/100 ml, 237 ng/100 ml, and less than 100 ng/100 ml, respectively. No adverse reactions were noted in any of the subjects; Papanicolaou smears remained class I, blood chemistry values showed no significant changes from pretreatment values, and no pregnancies occurred during treatment.", "PMID": 964403}
{"id": "pubmed23n0032_5917", "title": "Interaction of lectins with surface membrane receptors of animal cells. I. Factors responsible for agglutinability of human, rabbit, and sheep erythrocytes with concanavalin A.", "content": "A study of various factors influencing the Con A-mediated cell agglutinability failed to reveal any correlation between the agglutinability and the binding capacity of Con A-agglutinable and non-agglutinable human, rabbit, and sheep erythrocytes. Treatment with trypsin and neuraminidase on the one hand and chondroitin sulphate, polyvinylpyrrolidone and polylysine on the other hand made the agglutination of human erythrocytes possible, promoted the agglutination of rabbit erythrocytes, but had almost no effect on sheep erythrocytes, which agglutinated only after treatment with pronase or polylysine. The negligible effects of low temperature, NaN3, cytochalasine B and theophylline on cell agglutinability indicate that neither membrane fluidity, metabolic energy, cAMP, nor the microfilamentous apparatus are likely to play any important role in the Con A-mediated agglutination of erythrocytes. Differences in agglutinability between erythrocytes obtained from different animal species and subjected to different treatments with enzymes and polymers are explained as being due to alterations in the surface charge and the zeta potential.", "contents": "Interaction of lectins with surface membrane receptors of animal cells. I. Factors responsible for agglutinability of human, rabbit, and sheep erythrocytes with concanavalin A. A study of various factors influencing the Con A-mediated cell agglutinability failed to reveal any correlation between the agglutinability and the binding capacity of Con A-agglutinable and non-agglutinable human, rabbit, and sheep erythrocytes. Treatment with trypsin and neuraminidase on the one hand and chondroitin sulphate, polyvinylpyrrolidone and polylysine on the other hand made the agglutination of human erythrocytes possible, promoted the agglutination of rabbit erythrocytes, but had almost no effect on sheep erythrocytes, which agglutinated only after treatment with pronase or polylysine. The negligible effects of low temperature, NaN3, cytochalasine B and theophylline on cell agglutinability indicate that neither membrane fluidity, metabolic energy, cAMP, nor the microfilamentous apparatus are likely to play any important role in the Con A-mediated agglutination of erythrocytes. Differences in agglutinability between erythrocytes obtained from different animal species and subjected to different treatments with enzymes and polymers are explained as being due to alterations in the surface charge and the zeta potential.", "PMID": 964407}
{"id": "pubmed23n0032_5918", "title": "Enhancement of antibody response to soluble HSA antigen of young rabbits pretreated with deoxyribonucleic acids from adult donors.", "content": "Rabbits were injected with one dose of 2-4 mg. isolated nuclear DNA (from spleens of adult donors) at 3-5 days of age and their antibody response to a later administered soluble HSA antigen was followed. A) Rabbits receiving antigen only at 9 days of age, without previous DNA, did not form antibodies even after the second dose of HSA antigen given at 8 weeks of age. Nor were antibodies detected in the serum of rabbits treated before antigen, i.e., at 4-5 days of age, with DNA-N (from spleens of non-immunized rabbits), or DNA-P (from spleens of the pig immunized with a different antigen). Only rabbits treated with DNA-Ia (from spleens of rabbits immunized with soluble HSA antigen) formed antibodies to HSA antigen given at 9 days after birth. Antibodies were detectable in the serum at 21 days after antigen administration and retained increased levels as long as 50 days after the first dose of antigen. B) When HSA antigen was injected at 17-18 days after birth, even the DNA-N (4 mg.) given at 3-5 days after birth increased the antibody response in the recipients. Substantial acceleration and increase in antibody formation was obtained in rabbits treated with DNA-Ib after birth as compared to control rabbits (after antigen only with DNA). On the contrary, DNA-H from spleens of rabbits intensively immunized with HSA in adjuvant, given in a larger dose (4 mg.), suppressed the antibody response of rabbits over 8 weeks of age after the second dose of antigen. DNAs from spleens of immunized donors injected into rabbit offspring, without subsequent HSA antigen administration, did not lead to the appearance of detectable anti-HSA antibodies in the serum of young rabbits under the conditions of our experiments A and B. Long-term effects of DNAs injected in the postnatal period into rabbits on their immune response are discussed.", "contents": "Enhancement of antibody response to soluble HSA antigen of young rabbits pretreated with deoxyribonucleic acids from adult donors. Rabbits were injected with one dose of 2-4 mg. isolated nuclear DNA (from spleens of adult donors) at 3-5 days of age and their antibody response to a later administered soluble HSA antigen was followed. A) Rabbits receiving antigen only at 9 days of age, without previous DNA, did not form antibodies even after the second dose of HSA antigen given at 8 weeks of age. Nor were antibodies detected in the serum of rabbits treated before antigen, i.e., at 4-5 days of age, with DNA-N (from spleens of non-immunized rabbits), or DNA-P (from spleens of the pig immunized with a different antigen). Only rabbits treated with DNA-Ia (from spleens of rabbits immunized with soluble HSA antigen) formed antibodies to HSA antigen given at 9 days after birth. Antibodies were detectable in the serum at 21 days after antigen administration and retained increased levels as long as 50 days after the first dose of antigen. B) When HSA antigen was injected at 17-18 days after birth, even the DNA-N (4 mg.) given at 3-5 days after birth increased the antibody response in the recipients. Substantial acceleration and increase in antibody formation was obtained in rabbits treated with DNA-Ib after birth as compared to control rabbits (after antigen only with DNA). On the contrary, DNA-H from spleens of rabbits intensively immunized with HSA in adjuvant, given in a larger dose (4 mg.), suppressed the antibody response of rabbits over 8 weeks of age after the second dose of antigen. DNAs from spleens of immunized donors injected into rabbit offspring, without subsequent HSA antigen administration, did not lead to the appearance of detectable anti-HSA antibodies in the serum of young rabbits under the conditions of our experiments A and B. Long-term effects of DNAs injected in the postnatal period into rabbits on their immune response are discussed.", "PMID": 964408}
{"id": "pubmed23n0032_5919", "title": "Decreased in vitro incorporation of 3H-thymidine into spleen cell DNA of antigenically stimulated rabbits treated in the postnatal period with nucleic acid from adult donors.", "content": "Spleen cell DNA from 6-month-old rabbits, that has received the first dose of soluble HSA antigen at 9 days of age and the second at 2 months of age and did not form antibodies incorporated 3H-thymidine in vitro as early as 1 hour after restimulation of rabbits with HSA antigen in vivo. The incorporation rate (in muCi/mg.) of isolated cellular DNA was higher than that of DNA from rabbits that had been pretreated with nuclear DNA from the spleens of adult non-immunized rabbits or from the spleens of pigs immunized with a different antigen (DNP-BGG), 4-5 days before the first dose of antigen. The inhibitory effect of pig DNA was stronger. Decreased incorporation of DNA was noted at 8 hours after restimulation of rabbits treated in the same manner as the previous group. The difference between the incorporation rate at 1 hour and at 8 hours after the restimulation was most marked in the DNA obtained from rabbits not treated with nuclear DNA in the postnatal period. The activity of lymphoid cell DNA after antigenic stimulation is discussed.", "contents": "Decreased in vitro incorporation of 3H-thymidine into spleen cell DNA of antigenically stimulated rabbits treated in the postnatal period with nucleic acid from adult donors. Spleen cell DNA from 6-month-old rabbits, that has received the first dose of soluble HSA antigen at 9 days of age and the second at 2 months of age and did not form antibodies incorporated 3H-thymidine in vitro as early as 1 hour after restimulation of rabbits with HSA antigen in vivo. The incorporation rate (in muCi/mg.) of isolated cellular DNA was higher than that of DNA from rabbits that had been pretreated with nuclear DNA from the spleens of adult non-immunized rabbits or from the spleens of pigs immunized with a different antigen (DNP-BGG), 4-5 days before the first dose of antigen. The inhibitory effect of pig DNA was stronger. Decreased incorporation of DNA was noted at 8 hours after restimulation of rabbits treated in the same manner as the previous group. The difference between the incorporation rate at 1 hour and at 8 hours after the restimulation was most marked in the DNA obtained from rabbits not treated with nuclear DNA in the postnatal period. The activity of lymphoid cell DNA after antigenic stimulation is discussed.", "PMID": 964409}
{"id": "pubmed23n0032_5920", "title": "The intersex difference in the production of alloantibodies and growth of tumour allografts in mice.", "content": "The comparisons of immune responses to tumour allografts in male and female mice showed the following intersex differences: 1. Production of humoral antibodies is, in general, higher in females; since, however, the time course of titres differs for haemagglutinating and cytotoxic antibodies as well as with the sex, the titre of the same antibody may be higher in males at certain time intervals when the peak is delayed and the production in females is already declining. 2. In recipients specifically presensitized with spleen extract as well as in controls, the growth of allogeneic tumour (SaI) exhibits quantitative intersex differences being reduced in females. In the initial phase of growth, following the spleen extract, the facilitation (enhancement) of the tumour allograft is apparent olny in males, whereas in females it is delayed. 3. The facilitating effect of antibodies passively transferred from males or females corresponds mainly to the sex and strain of the recipient, only rarely to the sex of the producer. 4. In CBA strain mice, females are more resistant to the growth of SaI and also mortality from the primary tumour is lower in females; in contrast, the frequency of metastases in females is higher. 5. In the IC strain, where the sex effect on tumour growth is particularly pronounced, the consequences of castration were concordant with the inferred hormonal control of the allograft response: in males, the relatively high percentage of lethal spontaneous takes (60%) was reduced following castration (to 13%), whereas the opposite trend was observed in females, namely an increase from 0% in sham-operated controls to 23% in the castrated group.", "contents": "The intersex difference in the production of alloantibodies and growth of tumour allografts in mice. The comparisons of immune responses to tumour allografts in male and female mice showed the following intersex differences: 1. Production of humoral antibodies is, in general, higher in females; since, however, the time course of titres differs for haemagglutinating and cytotoxic antibodies as well as with the sex, the titre of the same antibody may be higher in males at certain time intervals when the peak is delayed and the production in females is already declining. 2. In recipients specifically presensitized with spleen extract as well as in controls, the growth of allogeneic tumour (SaI) exhibits quantitative intersex differences being reduced in females. In the initial phase of growth, following the spleen extract, the facilitation (enhancement) of the tumour allograft is apparent olny in males, whereas in females it is delayed. 3. The facilitating effect of antibodies passively transferred from males or females corresponds mainly to the sex and strain of the recipient, only rarely to the sex of the producer. 4. In CBA strain mice, females are more resistant to the growth of SaI and also mortality from the primary tumour is lower in females; in contrast, the frequency of metastases in females is higher. 5. In the IC strain, where the sex effect on tumour growth is particularly pronounced, the consequences of castration were concordant with the inferred hormonal control of the allograft response: in males, the relatively high percentage of lethal spontaneous takes (60%) was reduced following castration (to 13%), whereas the opposite trend was observed in females, namely an increase from 0% in sham-operated controls to 23% in the castrated group.", "PMID": 964410}
{"id": "pubmed23n0032_5921", "title": "Development of immunity against antigens of the A and B blood groups during postembryogenesis in chickens.", "content": "51Cr-labelled erythrocytes were injected into young chicks, F1 hybrids of the inbred chicken lines, from hatching to 2 months of age. The onset of immunity was measured by means of elimination of labelled red blood cells. It was found that with the B-locus difference between donors and recipients the immune response developed from 4 days of age to the end of the first week, whereas with the A-locus difference it developed during the second week of life. The onset of immunity is delayed in inbred chickens compared to outbred chickens, because we have used one alloantigen difference.", "contents": "Development of immunity against antigens of the A and B blood groups during postembryogenesis in chickens. 51Cr-labelled erythrocytes were injected into young chicks, F1 hybrids of the inbred chicken lines, from hatching to 2 months of age. The onset of immunity was measured by means of elimination of labelled red blood cells. It was found that with the B-locus difference between donors and recipients the immune response developed from 4 days of age to the end of the first week, whereas with the A-locus difference it developed during the second week of life. The onset of immunity is delayed in inbred chickens compared to outbred chickens, because we have used one alloantigen difference.", "PMID": 964411}
{"id": "pubmed23n0032_5922", "title": "[Increased immune reactivity of exacerbated visceral lupus erythematosus: II. Reason and pathogenetic significance].", "content": "The sera of patients suffering from acute phase Lupus erythematosus visceralis contains factors inducing an increased blastoid transformation of lymphocytes: 1. one or some factors having a molecular weight ranging from 7.8 X 10(5) to 1.5 X 10(6) daltons. This active principle was shown to increase specifically the transformation of only the autologous lymphocytes. These high-molecular weights components are supposed to be antigen-antibody-complexes. 2. Beside those high-molecular weight components also low-molecular weight factors could be shown to increase the transformation of lymphocytes as well. By contrast these latter factors stimulate the transformation of autologous as well as homologous lymphocytes. The low-molecular weights components are stable after an incubation for 30 min after 56 degrees C and have molecular weights of about 7.5 X 10(4) and 1.6 X 10(5) daltons, respectively. Supposed the low molecular weight factors of the sera of exacerbated Lupus erythematosus visceralis are mitogenic lymphokines the increased activity of those mediators should possess pathogenic significance.", "contents": "[Increased immune reactivity of exacerbated visceral lupus erythematosus: II. Reason and pathogenetic significance]. The sera of patients suffering from acute phase Lupus erythematosus visceralis contains factors inducing an increased blastoid transformation of lymphocytes: 1. one or some factors having a molecular weight ranging from 7.8 X 10(5) to 1.5 X 10(6) daltons. This active principle was shown to increase specifically the transformation of only the autologous lymphocytes. These high-molecular weights components are supposed to be antigen-antibody-complexes. 2. Beside those high-molecular weight components also low-molecular weight factors could be shown to increase the transformation of lymphocytes as well. By contrast these latter factors stimulate the transformation of autologous as well as homologous lymphocytes. The low-molecular weights components are stable after an incubation for 30 min after 56 degrees C and have molecular weights of about 7.5 X 10(4) and 1.6 X 10(5) daltons, respectively. Supposed the low molecular weight factors of the sera of exacerbated Lupus erythematosus visceralis are mitogenic lymphokines the increased activity of those mediators should possess pathogenic significance.", "PMID": 964423}
{"id": "pubmed23n0032_5923", "title": "[Hyde's nodular prurigo: an electronmicroscopic study].", "content": "Electron microscopic studies were carried out on cutaneous lesions in one case of prurigo nodularis Hyde. The cells of the basal layer partly showed large paranuclear vacuoles, which were sometimes divided by thin membrane structures. The intercellular space of the stratum basale and stratum spinosum was usually dilated, exhibiting acantholytic phenomen. Furthermore, numerous cells of these layers contain vacuolic-like mitochondria and polymorphous granular structures. Melanocytes and Langerhans-cells have not been observed, however, Merkel-cells were found in the basal layer, containing characteristic electrondense granules measuring 80 to 100 nm in diameter. The stratum granulosum frequently showed large intercellular complexes of numerous desmosomes, which sometimes were forming desmosomal bridges crossing the intercellular space. The capillaries in the dermis were usually widened and frequently surrounded by edematous alterations. The cytoplasm of the endothelial cells appeared very electrondens. Numerous unmyelinated axons were observed within the subepidermal zone containing mitochondria and single granules, measuring 80 to 100 nm in diameter.", "contents": "[Hyde's nodular prurigo: an electronmicroscopic study]. Electron microscopic studies were carried out on cutaneous lesions in one case of prurigo nodularis Hyde. The cells of the basal layer partly showed large paranuclear vacuoles, which were sometimes divided by thin membrane structures. The intercellular space of the stratum basale and stratum spinosum was usually dilated, exhibiting acantholytic phenomen. Furthermore, numerous cells of these layers contain vacuolic-like mitochondria and polymorphous granular structures. Melanocytes and Langerhans-cells have not been observed, however, Merkel-cells were found in the basal layer, containing characteristic electrondense granules measuring 80 to 100 nm in diameter. The stratum granulosum frequently showed large intercellular complexes of numerous desmosomes, which sometimes were forming desmosomal bridges crossing the intercellular space. The capillaries in the dermis were usually widened and frequently surrounded by edematous alterations. The cytoplasm of the endothelial cells appeared very electrondens. Numerous unmyelinated axons were observed within the subepidermal zone containing mitochondria and single granules, measuring 80 to 100 nm in diameter.", "PMID": 964424}
{"id": "pubmed23n0032_5924", "title": "Sensitization and testing of guinea pigs with nickel sulfate.", "content": "Experiments in passive transfer of nickel allergy require highly sensitive guinea pigs and a reliable and technically usable test method. A comparison was made between two sensitization methods, the guinea pig maximization test of MAGNUSSON and KLIGMAN (MK) and a combined injection and painting method devised by POLAK and TURK (PT). Intradermal injection was chosen as test method. The reactions to the test were greater than those of normal animals and the differences were statistically significant. With testing before the start of sensitization it was found difficult to obtain highly sensitive animals (tolerance). PT painting resulted in rather higher sensitivity with Triton X-100 than with sodium lauryl sulphate. In a comparison between the MK and PT methods, no difference in effectiveness was found provided that the animals had not been pretested. Repeated PT injections and paintings caused a further increase of the sensitivity, but the toxicity of nickel is a limiting factor.", "contents": "Sensitization and testing of guinea pigs with nickel sulfate. Experiments in passive transfer of nickel allergy require highly sensitive guinea pigs and a reliable and technically usable test method. A comparison was made between two sensitization methods, the guinea pig maximization test of MAGNUSSON and KLIGMAN (MK) and a combined injection and painting method devised by POLAK and TURK (PT). Intradermal injection was chosen as test method. The reactions to the test were greater than those of normal animals and the differences were statistically significant. With testing before the start of sensitization it was found difficult to obtain highly sensitive animals (tolerance). PT painting resulted in rather higher sensitivity with Triton X-100 than with sodium lauryl sulphate. In a comparison between the MK and PT methods, no difference in effectiveness was found provided that the animals had not been pretested. Repeated PT injections and paintings caused a further increase of the sensitivity, but the toxicity of nickel is a limiting factor.", "PMID": 964426}
{"id": "pubmed23n0032_5925", "title": "Acid hydrolase activity in normal human callus and in psoriatic scales.", "content": "The activities of 14 acid hydrolases have been measured in normal human callus and in psoriatic scales. Values for the scales were much higher than for callus. The increase is believed to be the result of two factors: an increased synthesis of these enzymes by the psoriatic lesion and a diminished degree of inactivation during the keratinization process.", "contents": "Acid hydrolase activity in normal human callus and in psoriatic scales. The activities of 14 acid hydrolases have been measured in normal human callus and in psoriatic scales. Values for the scales were much higher than for callus. The increase is believed to be the result of two factors: an increased synthesis of these enzymes by the psoriatic lesion and a diminished degree of inactivation during the keratinization process.", "PMID": 964428}
{"id": "pubmed23n0032_5926", "title": "Syphilis, homosexuality and legislation.", "content": "The proportion of cases with fresh syphilis in males contracted by homosexual contacts in Helsinki before and after the change of the criminal law in 1971 was studied. Since 1971, homosexuality is by law no longer a crime in Finland. In 1964, only 2% of males with fresh syphilis admitted a homosexual contact. The same figure was 8% in 1970, and increased to about 50% in 1974 and 1975. It was concluded that a change of legislation concerning homosexuality, probably by several different routes, changed the proportion of cases of detected homosexually transmitted early syphilis. This was thought to be of special importance for case finding and controlling of the spread of syphilis.", "contents": "Syphilis, homosexuality and legislation. The proportion of cases with fresh syphilis in males contracted by homosexual contacts in Helsinki before and after the change of the criminal law in 1971 was studied. Since 1971, homosexuality is by law no longer a crime in Finland. In 1964, only 2% of males with fresh syphilis admitted a homosexual contact. The same figure was 8% in 1970, and increased to about 50% in 1974 and 1975. It was concluded that a change of legislation concerning homosexuality, probably by several different routes, changed the proportion of cases of detected homosexually transmitted early syphilis. This was thought to be of special importance for case finding and controlling of the spread of syphilis.", "PMID": 964429}
{"id": "pubmed23n0032_5927", "title": "Modality differences in the habituation and dishabituation of cardiac responsiveness in the human newborn.", "content": "The relative efficacy of (1) repeated auditory and somesthetic stimulation for the habituation of cardiac acceleration responses and (2) intramodal and cross-modal stimulation for the dishabituation of cardiac responses was studied in 45 full-term 2-day-old infants. Although the stimuli were equally effective initially, repeated presentation of the somesthetic stimulus had a greater decremental effect than repeated presentation of the auditory stimulus. The stimuli were equally effective in producing dishabituation when in a different modality from that of the habituating stimulus (cross-modal) but not when in the same modality (intramodal). Changes in the locus of stimulation without a change in modality were ineffective for producing dishabituation. The findings indicate the human newborn discriminates auditory and somesthetic inputs effectively and equally but does not discriminate contralateral from ipsilateral stimulation in either modality.", "contents": "Modality differences in the habituation and dishabituation of cardiac responsiveness in the human newborn. The relative efficacy of (1) repeated auditory and somesthetic stimulation for the habituation of cardiac acceleration responses and (2) intramodal and cross-modal stimulation for the dishabituation of cardiac responses was studied in 45 full-term 2-day-old infants. Although the stimuli were equally effective initially, repeated presentation of the somesthetic stimulus had a greater decremental effect than repeated presentation of the auditory stimulus. The stimuli were equally effective in producing dishabituation when in a different modality from that of the habituating stimulus (cross-modal) but not when in the same modality (intramodal). Changes in the locus of stimulation without a change in modality were ineffective for producing dishabituation. The findings indicate the human newborn discriminates auditory and somesthetic inputs effectively and equally but does not discriminate contralateral from ipsilateral stimulation in either modality.", "PMID": 964456}
{"id": "pubmed23n0032_5928", "title": "Anatomical and behavioral effects of colchicine administration to rats late in utero.", "content": "Offspring from pregnant rats injected with 4mg/kg body weight colchicine on Embryonic Days 18, 19, and 20 were found to have isocortical and hippocampal structures greatly reduced in mass when examined at birth. Cells with pyknotic nuclei were found in Layers 5, 4, and 3 of the cerebral isocortex, the habenula, and anterior medial nuclei of the thalamus. Brains taken at Postnatal Days 22 and 132 were reduced in overall size, and had a 20-30% reduction of cells at the vertex of the neocortex with up to 50% reduction in the thickness of the corpus callosum. A decrease in activity, an increase in fearfulness and/or decreased tendency to explore, reduced error scores on the Hebb-Williams maze, poor performance on the Maier elevated maze, and a lessened sensitivity to sound-induced seizures were correlated with these anatomical changes.", "contents": "Anatomical and behavioral effects of colchicine administration to rats late in utero. Offspring from pregnant rats injected with 4mg/kg body weight colchicine on Embryonic Days 18, 19, and 20 were found to have isocortical and hippocampal structures greatly reduced in mass when examined at birth. Cells with pyknotic nuclei were found in Layers 5, 4, and 3 of the cerebral isocortex, the habenula, and anterior medial nuclei of the thalamus. Brains taken at Postnatal Days 22 and 132 were reduced in overall size, and had a 20-30% reduction of cells at the vertex of the neocortex with up to 50% reduction in the thickness of the corpus callosum. A decrease in activity, an increase in fearfulness and/or decreased tendency to explore, reduced error scores on the Hebb-Williams maze, poor performance on the Maier elevated maze, and a lessened sensitivity to sound-induced seizures were correlated with these anatomical changes.", "PMID": 964457}
{"id": "pubmed23n0032_5929", "title": "Weak inhibitory behavioral effects of postnatal! preweaning taurine injections in rats.", "content": "The behavior of rats injected once every 2 days between Postnatal Days 4 and 20 with 62.5 mug/g or 125 mug/g body weight of taurine was compared with saline- or .5 M NaCl-injected litter-mate controls. Taurine-injected rats ran significantly less in the running wheel test and displayed lower response/reinforcement ratios following step-like changes in DRL reinforcement schedules. The quicker adjustments to the changes in DRL schedules did not occur immediately but during sessions following the change. We concluded that taurine administration during early juvenile development could weakly influence adult inhibitory behavior.", "contents": "Weak inhibitory behavioral effects of postnatal! preweaning taurine injections in rats. The behavior of rats injected once every 2 days between Postnatal Days 4 and 20 with 62.5 mug/g or 125 mug/g body weight of taurine was compared with saline- or .5 M NaCl-injected litter-mate controls. Taurine-injected rats ran significantly less in the running wheel test and displayed lower response/reinforcement ratios following step-like changes in DRL reinforcement schedules. The quicker adjustments to the changes in DRL schedules did not occur immediately but during sessions following the change. We concluded that taurine administration during early juvenile development could weakly influence adult inhibitory behavior.", "PMID": 964458}
{"id": "pubmed23n0032_5930", "title": "Peculiarities in the changes of catecholamine level in the brain, blood, and adrenals of dogs and rats in ontogenesis.", "content": "The norepinephrine content of the whole brainstem and of its different parts and the epinephrine and norepinephrine (NE) content in the blood and adrenals in dogs and rats during ontogenesis were studied. The catecholamine (CA) level in the blood and different parts of the dog brainstem increased to the age of 20-30 days and then decreased. The NE value of the whole dog brainstem increased to the age of 9-30 days, and then decreased. The content of NE of the rat brainstem increased to the age of 120 days and then decreased. The amount of CA of the dog and rat adrenals increased progressively from birth to adulthood. The CA brain and adrenal level of dogs during postnatal ontogenesis was lower than in rats.", "contents": "Peculiarities in the changes of catecholamine level in the brain, blood, and adrenals of dogs and rats in ontogenesis. The norepinephrine content of the whole brainstem and of its different parts and the epinephrine and norepinephrine (NE) content in the blood and adrenals in dogs and rats during ontogenesis were studied. The catecholamine (CA) level in the blood and different parts of the dog brainstem increased to the age of 20-30 days and then decreased. The NE value of the whole dog brainstem increased to the age of 9-30 days, and then decreased. The content of NE of the rat brainstem increased to the age of 120 days and then decreased. The amount of CA of the dog and rat adrenals increased progressively from birth to adulthood. The CA brain and adrenal level of dogs during postnatal ontogenesis was lower than in rats.", "PMID": 964459}
{"id": "pubmed23n0032_5931", "title": "Visual sensitivity to orientation of rectangles in mice with retinal degeneration.", "content": "C3H/HeJ male mice, an inbred strain showing retinal degeneration (rd), and normal-eyed DBA/IJ male mice were studied in a task requiring discrimination of horizontally and vertically oriented rectangles. The apparatus was modified Yerkes discrimination apparatus. The mice were given 30 days of discrimination training, 10 trials/day, after which 10 more trails were given on the 31st day with positive and negative cues reversed. Both strains showed a highly significant decrease in errors during acquisition, the rd strain making significantly fewer errors than the DBA/IJ animals. Reversal of the cues brought about a sharp increase in percentage of errors.", "contents": "Visual sensitivity to orientation of rectangles in mice with retinal degeneration. C3H/HeJ male mice, an inbred strain showing retinal degeneration (rd), and normal-eyed DBA/IJ male mice were studied in a task requiring discrimination of horizontally and vertically oriented rectangles. The apparatus was modified Yerkes discrimination apparatus. The mice were given 30 days of discrimination training, 10 trials/day, after which 10 more trails were given on the 31st day with positive and negative cues reversed. Both strains showed a highly significant decrease in errors during acquisition, the rd strain making significantly fewer errors than the DBA/IJ animals. Reversal of the cues brought about a sharp increase in percentage of errors.", "PMID": 964460}
{"id": "pubmed23n0032_5932", "title": "The development of passive and active avoidance learning in the cat.", "content": "Kittens of 25 and 50 days of age were tested on a passive avoidance task in a 2-compartment black-white shuttle box. Latency to enter the dark side of the box was the same for both groups on Day 1, but the latency to enter the dark side on Day 2 was much shorter for the younger kittens. Other kittens were trained in a step-up active avoidance task also at 25 and 50 days of age. No differences were found in active avoidance performance between the younger and older animals, indicating that active and passive avoidance tasks may possibly reflect separate developmental processes that mature at different rates in the cat.", "contents": "The development of passive and active avoidance learning in the cat. Kittens of 25 and 50 days of age were tested on a passive avoidance task in a 2-compartment black-white shuttle box. Latency to enter the dark side of the box was the same for both groups on Day 1, but the latency to enter the dark side on Day 2 was much shorter for the younger kittens. Other kittens were trained in a step-up active avoidance task also at 25 and 50 days of age. No differences were found in active avoidance performance between the younger and older animals, indicating that active and passive avoidance tasks may possibly reflect separate developmental processes that mature at different rates in the cat.", "PMID": 964461}
{"id": "pubmed23n0032_5933", "title": "The pineal gland and the development of salt intake patterns in male rats.", "content": "The effect of gonadectomy, sham-gonadectomy, pinealectomy, and sham-pinealectomy on the concentration of NaCl in the total volume of fluid drunk per day ([NaCl]) in male rats offered a free choice between water and 3% saline was measured. Neonatal gonadectomy increased [NaCl] I in adult males but had no effect on immature rats. If the rats, however, were neonatally pinealectomized as well as gonadectomized, the increase of [NaCl] I also appeared in the immatures. Neonatal sham-pinealectomy decreased [NaCl] I in immature and adult rats, both gonadectomized and sham-gonadectomized. In adult neonatally gonadectomized males the effects of sham-pinealectomy did not occur in those animals actually pinealectomized. The role of the pineal gland in the regulation of water and salt intake is discussed in relation to the function of hypothalamus, neonatal stress, and sexual maturation.", "contents": "The pineal gland and the development of salt intake patterns in male rats. The effect of gonadectomy, sham-gonadectomy, pinealectomy, and sham-pinealectomy on the concentration of NaCl in the total volume of fluid drunk per day ([NaCl]) in male rats offered a free choice between water and 3% saline was measured. Neonatal gonadectomy increased [NaCl] I in adult males but had no effect on immature rats. If the rats, however, were neonatally pinealectomized as well as gonadectomized, the increase of [NaCl] I also appeared in the immatures. Neonatal sham-pinealectomy decreased [NaCl] I in immature and adult rats, both gonadectomized and sham-gonadectomized. In adult neonatally gonadectomized males the effects of sham-pinealectomy did not occur in those animals actually pinealectomized. The role of the pineal gland in the regulation of water and salt intake is discussed in relation to the function of hypothalamus, neonatal stress, and sexual maturation.", "PMID": 964462}
{"id": "pubmed23n0032_5934", "title": "The organization of sleep and wakefulness after maternal separation in young rats.", "content": "Two-week-old infant rats were studied before and after 24 hr of maternal absence. This experience produced an increase in time spent awake, a reduction in time spent in paradoxical sleep (PS), and an increased frequency of state transitions with more frequent and shorter periods of both slow-wave sleep (SWS) AND PS. Body movements were increased and nonnutritive sucking decreased during PS after separation. Other aspects of sleep organization were relativly stable: the pattern of probability of direction of state transitions, the integration of the physiological and behavioral determinants of state, the pattern and amount of nonnutritive sucking, and the underlying PS cycle period. These findings, and the results of 2 control experiments, suggest that some aspects of the prolonged absence of the mother produce a shift in the infant's central neutral state regulation through processes yet to be understood.", "contents": "The organization of sleep and wakefulness after maternal separation in young rats. Two-week-old infant rats were studied before and after 24 hr of maternal absence. This experience produced an increase in time spent awake, a reduction in time spent in paradoxical sleep (PS), and an increased frequency of state transitions with more frequent and shorter periods of both slow-wave sleep (SWS) AND PS. Body movements were increased and nonnutritive sucking decreased during PS after separation. Other aspects of sleep organization were relativly stable: the pattern of probability of direction of state transitions, the integration of the physiological and behavioral determinants of state, the pattern and amount of nonnutritive sucking, and the underlying PS cycle period. These findings, and the results of 2 control experiments, suggest that some aspects of the prolonged absence of the mother produce a shift in the infant's central neutral state regulation through processes yet to be understood.", "PMID": 964463}
{"id": "pubmed23n0032_5935", "title": "Acquisition and retention of habituation in the preweanling rat.", "content": "The acquisition and retention of habituation to air-puff was studied in rats on Days 1-19 postpartum. Animals of all ages demonstrated habituation but the rate of habituation varied with age: slower on each successive day from Day 1 to Day 8, but thereafter more rapid on each successive day. This peak of elicited responding is discussed in relation to the peak of spontaneous activity at Day 16 and to the pattern of neural development, including changes in acetylcholinesterase, norepinephrine, and serotonin, measured during the preweaning period. All ages showed 30-min retention of habituation, with 60-min retention developing after the 1st week. No evidence of 24-hr retention appeared at any age.", "contents": "Acquisition and retention of habituation in the preweanling rat. The acquisition and retention of habituation to air-puff was studied in rats on Days 1-19 postpartum. Animals of all ages demonstrated habituation but the rate of habituation varied with age: slower on each successive day from Day 1 to Day 8, but thereafter more rapid on each successive day. This peak of elicited responding is discussed in relation to the peak of spontaneous activity at Day 16 and to the pattern of neural development, including changes in acetylcholinesterase, norepinephrine, and serotonin, measured during the preweaning period. All ages showed 30-min retention of habituation, with 60-min retention developing after the 1st week. No evidence of 24-hr retention appeared at any age.", "PMID": 964464}
{"id": "pubmed23n0032_5936", "title": "Effects of neonatal thyroid hormone stimulation and differential preweaning rearing on open-field behavior in adult rats.", "content": "In 2 experiments, rats were given a mixture containing high doses of 1-tri-iodothyronine and 1-thyroxine-sodium on Day 4 after birth (Group T). Controls (C) received equivalent amounts of saline. During the preweaning period, half of the litters in the T and C groups were raised in an impoverished (I) condition, and half in an enriched (E) condition. Open-field ambulation, rearing, defecation, and, in Experiment 2, grooming behavior was recorded during a 4-day period at 210 days (Experiment 1) and at 90 days of age (Experiment 2). A consistent augmenting action of neonatal hormone stimulation on ambulation and rearing was found for both males and females. Hormone-treated animals did not habituate their rate of general motor activity with repeated testings in the open-field. An over-all pattern of a larger effect of hormone-treatment in the I condition than in the E condition was noted, particularly among the females. Thus, the experience of preweaning enrichment partly \"normalized\" the open-field behavior of the T groups. The results are discussed in relation to an hypothesis concerning the interaction of rate of development of the central nervous system and environmental stimulation in determining later behavior.", "contents": "Effects of neonatal thyroid hormone stimulation and differential preweaning rearing on open-field behavior in adult rats. In 2 experiments, rats were given a mixture containing high doses of 1-tri-iodothyronine and 1-thyroxine-sodium on Day 4 after birth (Group T). Controls (C) received equivalent amounts of saline. During the preweaning period, half of the litters in the T and C groups were raised in an impoverished (I) condition, and half in an enriched (E) condition. Open-field ambulation, rearing, defecation, and, in Experiment 2, grooming behavior was recorded during a 4-day period at 210 days (Experiment 1) and at 90 days of age (Experiment 2). A consistent augmenting action of neonatal hormone stimulation on ambulation and rearing was found for both males and females. Hormone-treated animals did not habituate their rate of general motor activity with repeated testings in the open-field. An over-all pattern of a larger effect of hormone-treatment in the I condition than in the E condition was noted, particularly among the females. Thus, the experience of preweaning enrichment partly \"normalized\" the open-field behavior of the T groups. The results are discussed in relation to an hypothesis concerning the interaction of rate of development of the central nervous system and environmental stimulation in determining later behavior.", "PMID": 964465}
{"id": "pubmed23n0032_5937", "title": "Growth in the Hubbard broiler: increased size following early handling.", "content": "Hubbard cockerels, 1 day of age (n = 91), were divided into 3 handling groups: (1) chicks from the 5-Day group were removed from the brooder and isolated at 22 degrees C for 3 min on each of the first 5 days; (2) chicks from the 15-Day group received this treatment for 15 days; and (3) Control chicks were not handled. Within each group half were fed broiler starter for 5 weeks, and half were fed layer starter which produces slower growth. On Days 28, 35, 42, and 49 the average weight of 5-Day birds was significantly higher than that of Controls; 15-Day birds weighed slightly less than birds in the 5-Day group. The weight advantage of the 5-Day birds occurred with both starter rations, at all weighing periods, and did not appear to reflect increased food consumption. Similarities between these findings and results obtained with rodents were noted.", "contents": "Growth in the Hubbard broiler: increased size following early handling. Hubbard cockerels, 1 day of age (n = 91), were divided into 3 handling groups: (1) chicks from the 5-Day group were removed from the brooder and isolated at 22 degrees C for 3 min on each of the first 5 days; (2) chicks from the 15-Day group received this treatment for 15 days; and (3) Control chicks were not handled. Within each group half were fed broiler starter for 5 weeks, and half were fed layer starter which produces slower growth. On Days 28, 35, 42, and 49 the average weight of 5-Day birds was significantly higher than that of Controls; 15-Day birds weighed slightly less than birds in the 5-Day group. The weight advantage of the 5-Day birds occurred with both starter rations, at all weighing periods, and did not appear to reflect increased food consumption. Similarities between these findings and results obtained with rodents were noted.", "PMID": 964466}
{"id": "pubmed23n0032_5938", "title": "Protein deprivation in primates. VIII. Early behavior of progeny.", "content": "Infant rhesus monkeys whose mothers were fed diets providing 1, 2, or 4 g of protein/kg body weight/day during pregnancy were tested on 13 behavioral tests during their first 60 days of life. (The diets had been shown to affect significantly the weights and blood protein concentrations of both pregnant and nonpregnant females.) The tests samples a wide range of behaviors, including visual and auditory perception, feeding reflexes, and locomotion. Despite the maternal dietary differences, the performances of infants on all tests were comparable in all groups. These data contrast with the finding of persistent deficit in rats born of protein-deprived mothers.", "contents": "Protein deprivation in primates. VIII. Early behavior of progeny. Infant rhesus monkeys whose mothers were fed diets providing 1, 2, or 4 g of protein/kg body weight/day during pregnancy were tested on 13 behavioral tests during their first 60 days of life. (The diets had been shown to affect significantly the weights and blood protein concentrations of both pregnant and nonpregnant females.) The tests samples a wide range of behaviors, including visual and auditory perception, feeding reflexes, and locomotion. Despite the maternal dietary differences, the performances of infants on all tests were comparable in all groups. These data contrast with the finding of persistent deficit in rats born of protein-deprived mothers.", "PMID": 964467}
{"id": "pubmed23n0032_5939", "title": "Visual evoked response (VER) changes during maturation in the Weddell seal.", "content": "Visual evoked responses (VER'S) were recorded from the cortex of immature Weddel seals, 3-365 days of age. Results indicated a high degree of maturity at birth evident from the multiphasic array of waveforms and the comparatively short onset latency of the VER. At low intensities, single flashes evoked an immature secondary response. Topographical distribution of VER's were confined largely to the gyrus immediately adjacent to midline, from the posterior aspect near lambda to the vertex. Recordings from CI-744 dosed seals displayed a well-demarcated developmental sequence of VER's, contrary to VER's recorded in flaxedilized seals. Onset latency and waveform configuration changed concomitantly as a function of age. During the postnatal period from birth to weaning, VER changes were related to major behavioral events such as the seal's first encounter with swimming and diving at 2 weeks of age and weaning at 6 weeks of age.", "contents": "Visual evoked response (VER) changes during maturation in the Weddell seal. Visual evoked responses (VER'S) were recorded from the cortex of immature Weddel seals, 3-365 days of age. Results indicated a high degree of maturity at birth evident from the multiphasic array of waveforms and the comparatively short onset latency of the VER. At low intensities, single flashes evoked an immature secondary response. Topographical distribution of VER's were confined largely to the gyrus immediately adjacent to midline, from the posterior aspect near lambda to the vertex. Recordings from CI-744 dosed seals displayed a well-demarcated developmental sequence of VER's, contrary to VER's recorded in flaxedilized seals. Onset latency and waveform configuration changed concomitantly as a function of age. During the postnatal period from birth to weaning, VER changes were related to major behavioral events such as the seal's first encounter with swimming and diving at 2 weeks of age and weaning at 6 weeks of age.", "PMID": 964468}
{"id": "pubmed23n0032_5940", "title": "Gastrointestinal myoelectrical activity during the postoperative period in man.", "content": "Myoelectrical activity of the gastrointestinal tract has been studied in the postoperative period of 13 patients who underwent cholecystectomy. The recordings have been performed by means of extracellular electrodes which were implanted at the levels of stomach, jejunum, ileum and colon during the surgical procedure. The records showed that fast activity is always persistent while the basic electrical rhythm is greatly disorganized during the immediate postoperative period. Such a characteristic pattern of the electrical activity suggested that the lack of peristaltic and propulsive movements, always noted during this period, is not correlated with a disappearance of gastrointestinal contractions, but only with a disturbance in their coordination.", "contents": "Gastrointestinal myoelectrical activity during the postoperative period in man. Myoelectrical activity of the gastrointestinal tract has been studied in the postoperative period of 13 patients who underwent cholecystectomy. The recordings have been performed by means of extracellular electrodes which were implanted at the levels of stomach, jejunum, ileum and colon during the surgical procedure. The records showed that fast activity is always persistent while the basic electrical rhythm is greatly disorganized during the immediate postoperative period. Such a characteristic pattern of the electrical activity suggested that the lack of peristaltic and propulsive movements, always noted during this period, is not correlated with a disappearance of gastrointestinal contractions, but only with a disturbance in their coordination.", "PMID": 964469}
{"id": "pubmed23n0032_5941", "title": "Carcinomas of the colon in Buffalo strain rats given intraperitoneal injections of methylazoxymethanol acetate.", "content": "Buffalo strain male and female rats were given methylazoxymethanol acetate (20% solution) intraperitoneally 20 mg/kg body weight weekly for 9 weeks, and killed 27 weeks later. Carcinomas of the large intestine, predominantly in the descending colon, developed in some of the rats. The incidence was higher in male than in female rats. Polypoid carcinomas were observed more often in male rats and sessile carcinomas with metastases in the female rats. Carcinomas were not observed in organs other than the large intestine.", "contents": "Carcinomas of the colon in Buffalo strain rats given intraperitoneal injections of methylazoxymethanol acetate. Buffalo strain male and female rats were given methylazoxymethanol acetate (20% solution) intraperitoneally 20 mg/kg body weight weekly for 9 weeks, and killed 27 weeks later. Carcinomas of the large intestine, predominantly in the descending colon, developed in some of the rats. The incidence was higher in male than in female rats. Polypoid carcinomas were observed more often in male rats and sessile carcinomas with metastases in the female rats. Carcinomas were not observed in organs other than the large intestine.", "PMID": 964470}
{"id": "pubmed23n0032_5942", "title": "Transit time changes with age in the gastrointestinal tract of the rat.", "content": "Using 85Sr-labelled microspheres as nonabsorbable markers, the effect of age on gastrointestinal propulsion motility was determined in conscious rats. There was no significant correlation between the gastric emptying rate or the propulsion motility of the small intestine and the age of the rats. However, the transit time of the intestinal content in the caecum in adult (17- to 20-month-old) rats was about fivefold the value found in weanling (19-day-old) rats. Also the transit time of the large intestine was found to be prolonged by about 70% during the life span investigated.", "contents": "Transit time changes with age in the gastrointestinal tract of the rat. Using 85Sr-labelled microspheres as nonabsorbable markers, the effect of age on gastrointestinal propulsion motility was determined in conscious rats. There was no significant correlation between the gastric emptying rate or the propulsion motility of the small intestine and the age of the rats. However, the transit time of the intestinal content in the caecum in adult (17- to 20-month-old) rats was about fivefold the value found in weanling (19-day-old) rats. Also the transit time of the large intestine was found to be prolonged by about 70% during the life span investigated.", "PMID": 964471}
{"id": "pubmed23n0032_5943", "title": "Effects of L-glutamine on various gastric lesions in rats and guinea pigs.", "content": "Effects of L-glutamine on gastric lesion models in rats and guinea pigs were studied. Shay ulceration in rats was not inhibited by oral L-glutamine. Although stress-induced gastric lesions in intact rats were not inhibited by L-glutamine, a strong antagonism of gastric lesions was induced in pylorus-ligated rats under the same stress by the amino acid. Histamine-induced gastric lesions in intact rats and guinea pigs were also markedly inhibited by L-glutamine. L-Glutamine inhibited the gastric lesions in rats induced by compound 48/80 but not significantly. Reserpine- or serotonin-induced gastric lesions in rats were not affected by L-glutamine. The mechanism of L-glutamine protection was discussed.", "contents": "Effects of L-glutamine on various gastric lesions in rats and guinea pigs. Effects of L-glutamine on gastric lesion models in rats and guinea pigs were studied. Shay ulceration in rats was not inhibited by oral L-glutamine. Although stress-induced gastric lesions in intact rats were not inhibited by L-glutamine, a strong antagonism of gastric lesions was induced in pylorus-ligated rats under the same stress by the amino acid. Histamine-induced gastric lesions in intact rats and guinea pigs were also markedly inhibited by L-glutamine. L-Glutamine inhibited the gastric lesions in rats induced by compound 48/80 but not significantly. Reserpine- or serotonin-induced gastric lesions in rats were not affected by L-glutamine. The mechanism of L-glutamine protection was discussed.", "PMID": 964472}
{"id": "pubmed23n0032_5944", "title": "The role of the digestive tract in cholesterol metabolism.", "content": "New studies have permit to reevaluate the importance of the digestive tract in each of the mechanisms regulating cholesterol turnover. Particularly, the role of the digestive trace in the rat cholesterol synthesis was underestimated. Is there a similar situation in man?", "contents": "The role of the digestive tract in cholesterol metabolism. New studies have permit to reevaluate the importance of the digestive tract in each of the mechanisms regulating cholesterol turnover. Particularly, the role of the digestive trace in the rat cholesterol synthesis was underestimated. Is there a similar situation in man?", "PMID": 964473}
{"id": "pubmed23n0032_5945", "title": "Gastric clearance of serum albumin in patients with alcoholic liver cirrhosis.", "content": "Gastric albumin clearance studies were carried out in 26 patients with alcoholic cirrhosis and in 10 control subjects. The mean clearance was significantly higher in patients than in control subjects. An increased protein gastric loss in cirrhotic patients was observed only in presence of gastritis. The presence or absence of distended esophageal varices did not influence the protein loss.", "contents": "Gastric clearance of serum albumin in patients with alcoholic liver cirrhosis. Gastric albumin clearance studies were carried out in 26 patients with alcoholic cirrhosis and in 10 control subjects. The mean clearance was significantly higher in patients than in control subjects. An increased protein gastric loss in cirrhotic patients was observed only in presence of gastritis. The presence or absence of distended esophageal varices did not influence the protein loss.", "PMID": 964474}
{"id": "pubmed23n0032_5946", "title": "High serum glutamic acid levels in patients with carcinoma of the pancreas.", "content": "High levels of glutamic acid and normal levels of glutamine were found in the blood of all but one of ten patients with carcinoma of the pancreas. No changes of the concentration in blood of these two amino acids were observed in 49 patients suffering from several diseases including malignant tumours. Although the mechanism of the rise of serum glutamic acid is difficult to explain at present, it may have some diagnostic value.", "contents": "High serum glutamic acid levels in patients with carcinoma of the pancreas. High levels of glutamic acid and normal levels of glutamine were found in the blood of all but one of ten patients with carcinoma of the pancreas. No changes of the concentration in blood of these two amino acids were observed in 49 patients suffering from several diseases including malignant tumours. Although the mechanism of the rise of serum glutamic acid is difficult to explain at present, it may have some diagnostic value.", "PMID": 964475}
{"id": "pubmed23n0032_5947", "title": "Serum gastric concentration after sham feeding and feeding under the influence of propranolol in man.", "content": "The influence of propranolol, 50 mug/kg administered intravenously, on the gastrin concentration during sham feeding and after a test meal was studied in eight normal subjects. beta-Adrenergic blockade had no inhibitory effect on the gastrin response to feeding. Sham feeding did not provoke a significant rise of serum concentration; however, in three subjects a definite gastrin response occurred in the presence of beta-adrenergic blockade.", "contents": "Serum gastric concentration after sham feeding and feeding under the influence of propranolol in man. The influence of propranolol, 50 mug/kg administered intravenously, on the gastrin concentration during sham feeding and after a test meal was studied in eight normal subjects. beta-Adrenergic blockade had no inhibitory effect on the gastrin response to feeding. Sham feeding did not provoke a significant rise of serum concentration; however, in three subjects a definite gastrin response occurred in the presence of beta-adrenergic blockade.", "PMID": 964476}
{"id": "pubmed23n0032_5948", "title": "Gastric secretory response to cholinergic stimulation in rats chronically pretreated with pentagastrin or histamine in depot form.", "content": "Rats pretreated by daily depot injections of pentagastric for 24 days, responded with a significant increase of gastric secretion volume, and HC1 concentration and output, when infused intravenously with bethanechol chloride. In these rats, the total weight of the stomach and the height of the fundic mucosa were also increased. Pretreatment with histamine had no effect on rat gastric tissue or gastric secretion, under identical conditions of stimulation.", "contents": "Gastric secretory response to cholinergic stimulation in rats chronically pretreated with pentagastrin or histamine in depot form. Rats pretreated by daily depot injections of pentagastric for 24 days, responded with a significant increase of gastric secretion volume, and HC1 concentration and output, when infused intravenously with bethanechol chloride. In these rats, the total weight of the stomach and the height of the fundic mucosa were also increased. Pretreatment with histamine had no effect on rat gastric tissue or gastric secretion, under identical conditions of stimulation.", "PMID": 964477}
{"id": "pubmed23n0032_5949", "title": "The effect of intravenous infusion of synthetic human gastrin-I on lower esophageal sphincter (LES) pressure in the dog and its relation to gastrin level.", "content": "To demonstrate the relation between gastrin level and lower esophageal sphincter (LES) pressure, experiments with intravenous infusions of synthetic human gastrin-I (SHG-I) were performed in anaesthetized dogs. The results show that infusions with 5 mug-kg-1-h-1 SHG-I have an initially increasing effect on LES pressure but produce unphysiologically high gastrin blood levels. Physiological levels of gastrin were measured only using an infusion dose of 0.5 mug-kg-1-h-1, which was below the threshold for increasing LES pressure.", "contents": "The effect of intravenous infusion of synthetic human gastrin-I on lower esophageal sphincter (LES) pressure in the dog and its relation to gastrin level. To demonstrate the relation between gastrin level and lower esophageal sphincter (LES) pressure, experiments with intravenous infusions of synthetic human gastrin-I (SHG-I) were performed in anaesthetized dogs. The results show that infusions with 5 mug-kg-1-h-1 SHG-I have an initially increasing effect on LES pressure but produce unphysiologically high gastrin blood levels. Physiological levels of gastrin were measured only using an infusion dose of 0.5 mug-kg-1-h-1, which was below the threshold for increasing LES pressure.", "PMID": 964478}
{"id": "pubmed23n0032_5950", "title": "Histamine-stimulated gastric secretion in rats with chronic renal insufficiency.", "content": "Chronic uremia was induced by long-term maintenance of rats which had undergone sub-total nephrectomy, with sham-operated rats serving as controls. Chronic gastric cannulas and chronic vascular catheters (vena cava) were installed and the rats were stimulated with intravenous infusion of histamine. In uremic rats, the volume of gastric juice, output of HC1 and H+ concentration were significantly higher than in the controls during the 6-hour secretion experiments.", "contents": "Histamine-stimulated gastric secretion in rats with chronic renal insufficiency. Chronic uremia was induced by long-term maintenance of rats which had undergone sub-total nephrectomy, with sham-operated rats serving as controls. Chronic gastric cannulas and chronic vascular catheters (vena cava) were installed and the rats were stimulated with intravenous infusion of histamine. In uremic rats, the volume of gastric juice, output of HC1 and H+ concentration were significantly higher than in the controls during the 6-hour secretion experiments.", "PMID": 964479}
{"id": "pubmed23n0032_5951", "title": "Serum proinsulin in normal and gestational diabetic pregnancy.", "content": "The concentration of proinsulin-like components (PLC) in serum has been determined by gel filtration on samples obtained from eight normal pregnant women and eight nonobese gestational diabetics. The normal women were investigated early in pregnancy and all subjects were investigated in mid pregnancy, late pregnancy, and postpartum. At each occasion, samples were obtained after an overnight fast and after glucose ingestion. In both groups, the concentration of PLC in serum after overnight fast rose with gestation as well as after glucose ingestion, but there were no significant differences between mean levels of PLC of the normals and the gestational diabetics. With gestation, serum insulin rose in parallel with PLC in either group. The proportion of total insulin immunoreactivity composed by PLC thus remained constant and, furthermore, the proportions of PLC in gestation were equal to those observed postpartum. Four to six weeks after delivery, the basal concentration of PLC in serum was higher in the gestational diabetics than in the normals, whereas the concentrations of insulin were equal. Since the biological potency of proinsulin is much less than that of insulin, the results exclude the possibility that the decrease of glucose tolerance in normal pregnant women and gestational diabetics is due to an increased concentration of proinsulin in serum.", "contents": "Serum proinsulin in normal and gestational diabetic pregnancy. The concentration of proinsulin-like components (PLC) in serum has been determined by gel filtration on samples obtained from eight normal pregnant women and eight nonobese gestational diabetics. The normal women were investigated early in pregnancy and all subjects were investigated in mid pregnancy, late pregnancy, and postpartum. At each occasion, samples were obtained after an overnight fast and after glucose ingestion. In both groups, the concentration of PLC in serum after overnight fast rose with gestation as well as after glucose ingestion, but there were no significant differences between mean levels of PLC of the normals and the gestational diabetics. With gestation, serum insulin rose in parallel with PLC in either group. The proportion of total insulin immunoreactivity composed by PLC thus remained constant and, furthermore, the proportions of PLC in gestation were equal to those observed postpartum. Four to six weeks after delivery, the basal concentration of PLC in serum was higher in the gestational diabetics than in the normals, whereas the concentrations of insulin were equal. Since the biological potency of proinsulin is much less than that of insulin, the results exclude the possibility that the decrease of glucose tolerance in normal pregnant women and gestational diabetics is due to an increased concentration of proinsulin in serum.", "PMID": 964507}
{"id": "pubmed23n0032_5952", "title": "Serum insulin levels in school children aged 9--12 in Westland, Holland.", "content": "In a study of risk factors for cardiovascular disease in 2388 school children aged 9--12 years carried out in Westland, Holland, serum insulin levels at one hour after an oral challenge of 50 g glucose were measured in a systematically selected subsample of 715 children. The distribution and associations of serum insulin in these children are described. The mean insulin values were 24.6 muU/ml for boys and 32.0 muU/ml for girls. The difference between these means was statistically significant and remained so even taking measures of adiposity into account. Insulin values were positively related to levels of plasma sugar and systolic blood pressure in both sexes.", "contents": "Serum insulin levels in school children aged 9--12 in Westland, Holland. In a study of risk factors for cardiovascular disease in 2388 school children aged 9--12 years carried out in Westland, Holland, serum insulin levels at one hour after an oral challenge of 50 g glucose were measured in a systematically selected subsample of 715 children. The distribution and associations of serum insulin in these children are described. The mean insulin values were 24.6 muU/ml for boys and 32.0 muU/ml for girls. The difference between these means was statistically significant and remained so even taking measures of adiposity into account. Insulin values were positively related to levels of plasma sugar and systolic blood pressure in both sexes.", "PMID": 964508}
{"id": "pubmed23n0032_5953", "title": "Effects of exogenous hormones and glucose on plasma levels and hepatic metabolism of amino acids in the fetus and in the newborn rat.", "content": "The present study examines the role of insulin, glucagon and cortisol in the regulation of gluconeogenesis from lactate and amino acids in fetal and newborn rats. Injection of glucagon in the full-term fetal rat caused a rise in glucose (and insulin) and a fall in blood levels of most individual amino acids, stimulated hepatic accumulation of 14C-amino isobutyric acid and 14C-cycloleucine and increased the conversion of 14C lactate, alanine and serine to glucose in vivo and in vitro (liver slices). Such changes were equivalent to the changes seen in 4 h old newborn rats. When glucagon was administered at birth, little difference was observed between control and treated animals in plasma amino acids and a smaller increment in conversion of 14C substrate to glucose occurred. By contrast, insulin injection at birth caused hypoglycemia, suppression of levels of certain amino acids and inhibition of conversion of 14C substrates into glucose. Glucose injection at birth caused elevated glycemia and plasma insulin and suppression of most amino acid levels and of conversion of 14C substrate into glucose. Cortisol injection at birth caused a marked, generalized by hyperaminoacidemia, a stimulation of glucagon secretion and of conversion of 14C substrates into glucose. These observations support the thesis that glucagon plays a major role in the induction of hepatic gluconeogenesis and that insulin acts as an antagonist hormone.", "contents": "Effects of exogenous hormones and glucose on plasma levels and hepatic metabolism of amino acids in the fetus and in the newborn rat. The present study examines the role of insulin, glucagon and cortisol in the regulation of gluconeogenesis from lactate and amino acids in fetal and newborn rats. Injection of glucagon in the full-term fetal rat caused a rise in glucose (and insulin) and a fall in blood levels of most individual amino acids, stimulated hepatic accumulation of 14C-amino isobutyric acid and 14C-cycloleucine and increased the conversion of 14C lactate, alanine and serine to glucose in vivo and in vitro (liver slices). Such changes were equivalent to the changes seen in 4 h old newborn rats. When glucagon was administered at birth, little difference was observed between control and treated animals in plasma amino acids and a smaller increment in conversion of 14C substrate to glucose occurred. By contrast, insulin injection at birth caused hypoglycemia, suppression of levels of certain amino acids and inhibition of conversion of 14C substrates into glucose. Glucose injection at birth caused elevated glycemia and plasma insulin and suppression of most amino acid levels and of conversion of 14C substrate into glucose. Cortisol injection at birth caused a marked, generalized by hyperaminoacidemia, a stimulation of glucagon secretion and of conversion of 14C substrates into glucose. These observations support the thesis that glucagon plays a major role in the induction of hepatic gluconeogenesis and that insulin acts as an antagonist hormone.", "PMID": 964509}
{"id": "pubmed23n0032_5954", "title": "Circadian variation of serum glucose, C-peptide immunoreactivity and free insulin normal and insulin-treated diabetic pregnant subjects.", "content": "To examine differences among pregnant diabetic and nondiabetic subjects, serum glucose, and immunoreactivity of C-peptide, free and total insulin were measured at hourly intervals during a 24--h third trimester metabolic ward evaluation. Six normals, three mild, and four juvenile-onset type diabetics were studied. Diets were identical for all subjects. Mild diabetics differed from juvenile diabetics by having significant residual pancreatic B-cell function, as measured by C-peptide immunoreactivity. Short and intermediate acting insulins given once or twice daily to diabetics maintained serum glucose levels within the normal range throughout the 24 h. Despite wide variation in serum total insulin levels, peripheral free insulin concentrations in well-controlled diabetics fell within a relatively narrow range that was higher than in controls. Infants of the diabetic subjects were comparable to the offpsring of the control women.", "contents": "Circadian variation of serum glucose, C-peptide immunoreactivity and free insulin normal and insulin-treated diabetic pregnant subjects. To examine differences among pregnant diabetic and nondiabetic subjects, serum glucose, and immunoreactivity of C-peptide, free and total insulin were measured at hourly intervals during a 24--h third trimester metabolic ward evaluation. Six normals, three mild, and four juvenile-onset type diabetics were studied. Diets were identical for all subjects. Mild diabetics differed from juvenile diabetics by having significant residual pancreatic B-cell function, as measured by C-peptide immunoreactivity. Short and intermediate acting insulins given once or twice daily to diabetics maintained serum glucose levels within the normal range throughout the 24 h. Despite wide variation in serum total insulin levels, peripheral free insulin concentrations in well-controlled diabetics fell within a relatively narrow range that was higher than in controls. Infants of the diabetic subjects were comparable to the offpsring of the control women.", "PMID": 964510}
{"id": "pubmed23n0032_5955", "title": "Thermosensitivity of nuclear RNA polymerase during the in vitro senescence of mouse fibroblasts.", "content": "Embryonic mouse fibroblasts divide approximately twelve times in vitro prior to cessation of mitotic activity. During this period of cellular senescence the thermosensitivity of the RNA polymerase activity of isolated nuclei has been examined as a means of detecting the possible accumulation of defective enzyme molecules, as has been found by other workers for several cytoplasmic enzymes during the ageing of human fibroblasts in vitro. The total RNA polymerase activity of nuclei isolated from old (10th generation) cells is more thermoresistant than that of young (2nd generation) cells. However, the net RNA polymerase activity of nuclei from non-dividing (confluent) cells is more thermoresistant than that of exponentially growing cells of the same age. When allowance is made for the state of growth of the cultures, little difference is seens in the thermosensitivity of the activities of nuclei from old and young cells. Neither is there any difference between the thermosensitivity of the net activity of an established line of murine fibroblasts (L-cells) and cells in primary culture. Preheating nuclei increases the inhibition of their total RNA polymerase activity by alpha-amanitin, indicating that RNA polymerase II is the most heat resistance species present. There appears to be no difference between the thermosensitivity of the alpha-amanitin sensitive and resistance species of the enzyme in the nuclei of old and young cells. It is concluded that old cells resemble non-dividing young cells in containing a higher proportion of RNA polymerase II in their nuclei, resulting in greater thermoresistance of the total RNA polymerase activity over that of exponentially growing cells. However, there appears to be no increase in thermosensitivity of the enzymes with age.", "contents": "Thermosensitivity of nuclear RNA polymerase during the in vitro senescence of mouse fibroblasts. Embryonic mouse fibroblasts divide approximately twelve times in vitro prior to cessation of mitotic activity. During this period of cellular senescence the thermosensitivity of the RNA polymerase activity of isolated nuclei has been examined as a means of detecting the possible accumulation of defective enzyme molecules, as has been found by other workers for several cytoplasmic enzymes during the ageing of human fibroblasts in vitro. The total RNA polymerase activity of nuclei isolated from old (10th generation) cells is more thermoresistant than that of young (2nd generation) cells. However, the net RNA polymerase activity of nuclei from non-dividing (confluent) cells is more thermoresistant than that of exponentially growing cells of the same age. When allowance is made for the state of growth of the cultures, little difference is seens in the thermosensitivity of the activities of nuclei from old and young cells. Neither is there any difference between the thermosensitivity of the net activity of an established line of murine fibroblasts (L-cells) and cells in primary culture. Preheating nuclei increases the inhibition of their total RNA polymerase activity by alpha-amanitin, indicating that RNA polymerase II is the most heat resistance species present. There appears to be no difference between the thermosensitivity of the alpha-amanitin sensitive and resistance species of the enzyme in the nuclei of old and young cells. It is concluded that old cells resemble non-dividing young cells in containing a higher proportion of RNA polymerase II in their nuclei, resulting in greater thermoresistance of the total RNA polymerase activity over that of exponentially growing cells. However, there appears to be no increase in thermosensitivity of the enzymes with age.", "PMID": 964511}
{"id": "pubmed23n0032_5956", "title": "Embryo cell surfaces--lectin binding and cell proliferation.", "content": "The interaction between chick embryo fibroblasts and various lectins has been studied at different stages of embryo development. There is evidence that Robinia lectin, Dolichos lectin, and Concanavalin A decrease cell number and proportion of cells incorporating [3H] thymidine in case of 8- and 10-day-old chick embryo fibroblasts, whereas they stimulated the proliferation of 16-day-old embryo cells. No effect was noticed in 12-day cells. These results suggest that some cell surface changes occur during embryo development. The site number of Dolichos lectin remains the same during embryo development, and the affinity constant decreases. The site number of Robinia lectin and Concanavalin A decreases from the 8th to the 12th day of development, and slowly increases on the 16-day cells, the affinity constant remaining rather constant. The results indicate that the age-dependent effect of lectin on embryo cells could not be directly related to the number of lectin-binding sites. Competitive binding experiments revealed that Dolichos receptor sites were distincts from binding sites of Robinia lectin and Concanavalin A, and Robina receptor sites distinct from those of concanavalin A. Lectin effects on embryo fibroblasts were very specific as determined by inhibitory assays.", "contents": "Embryo cell surfaces--lectin binding and cell proliferation. The interaction between chick embryo fibroblasts and various lectins has been studied at different stages of embryo development. There is evidence that Robinia lectin, Dolichos lectin, and Concanavalin A decrease cell number and proportion of cells incorporating [3H] thymidine in case of 8- and 10-day-old chick embryo fibroblasts, whereas they stimulated the proliferation of 16-day-old embryo cells. No effect was noticed in 12-day cells. These results suggest that some cell surface changes occur during embryo development. The site number of Dolichos lectin remains the same during embryo development, and the affinity constant decreases. The site number of Robinia lectin and Concanavalin A decreases from the 8th to the 12th day of development, and slowly increases on the 16-day cells, the affinity constant remaining rather constant. The results indicate that the age-dependent effect of lectin on embryo cells could not be directly related to the number of lectin-binding sites. Competitive binding experiments revealed that Dolichos receptor sites were distincts from binding sites of Robinia lectin and Concanavalin A, and Robina receptor sites distinct from those of concanavalin A. Lectin effects on embryo fibroblasts were very specific as determined by inhibitory assays.", "PMID": 964512}
{"id": "pubmed23n0032_5957", "title": "Carcinoplacental alkaline phosphatase. Base level and hormone-induced activity associated with events in the cell cycle.", "content": "Using HeLa TCRC-1, a cell line which is monophenotypic with respect to the Regan isoenzyme of alkaline phosphatase, we have examined the factors which influence its expression in relation to events of the cell cycle. DNA synthesis is not required for hormone induction of the Regan isoenzyme as in the presence of hydroxyurea, a specific inhibitor of DNA synthesis, we found induction to occur. Additionally, when partially synchronised cells were allowed to leave the S period prior to hormone treatment, and hydroxyurea was added to prevent cells from entering the next S period, hormone induction of the Regan isoenzyme was still observed. This indicates that initiation of expression of hormone-induced carcinoplacental alkaline phosphatase occur prior to the DNA synthetic phase of the cell cycle. We propose a hypothetical two-step mechanism of hormone induction to interpret the present findings in relation to previous results.", "contents": "Carcinoplacental alkaline phosphatase. Base level and hormone-induced activity associated with events in the cell cycle. Using HeLa TCRC-1, a cell line which is monophenotypic with respect to the Regan isoenzyme of alkaline phosphatase, we have examined the factors which influence its expression in relation to events of the cell cycle. DNA synthesis is not required for hormone induction of the Regan isoenzyme as in the presence of hydroxyurea, a specific inhibitor of DNA synthesis, we found induction to occur. Additionally, when partially synchronised cells were allowed to leave the S period prior to hormone treatment, and hydroxyurea was added to prevent cells from entering the next S period, hormone induction of the Regan isoenzyme was still observed. This indicates that initiation of expression of hormone-induced carcinoplacental alkaline phosphatase occur prior to the DNA synthetic phase of the cell cycle. We propose a hypothetical two-step mechanism of hormone induction to interpret the present findings in relation to previous results.", "PMID": 964513}
{"id": "pubmed23n0032_5958", "title": "Decidualisation in the prepuberal rat uterus.", "content": "Decidualisation has been experimentally induced in the prepuberal rat. It is morphologically similar to that obtained in the adult. The cytodifferentiation includes the formation of binucleated cells and extensive specialised cellular junctions. Furthermore, blastocysts from adults transferred into uterine horns of immature specimens implant and develop, indicating that the decidual response they induce is functionally comparable to the uterine response at the time of nidation in the fertilised adult. The model of the experimental decidual induction in the immature is used to study the ontogenic processes of uterine maturation. Preliminary observations indicate that the 7-10-days period might be of significance in the maturation of the rat uterus.", "contents": "Decidualisation in the prepuberal rat uterus. Decidualisation has been experimentally induced in the prepuberal rat. It is morphologically similar to that obtained in the adult. The cytodifferentiation includes the formation of binucleated cells and extensive specialised cellular junctions. Furthermore, blastocysts from adults transferred into uterine horns of immature specimens implant and develop, indicating that the decidual response they induce is functionally comparable to the uterine response at the time of nidation in the fertilised adult. The model of the experimental decidual induction in the immature is used to study the ontogenic processes of uterine maturation. Preliminary observations indicate that the 7-10-days period might be of significance in the maturation of the rat uterus.", "PMID": 964514}
{"id": "pubmed23n0032_5959", "title": "The effect of isoproterenol upon the chemical composition of plasma membranes in the mouse parotid gland.", "content": "A single intraperitoneal injection of isoproterenol induces resting cells from the acini of the mouse parotid gland to enter the proliferative cycle. Parotid plasma membrane from non-stimulated and isoproterenol-treated mice were prepared by differential centrifugation of the homogenates. Comparing the chemical composition of plasma membranes from non-stimulated and isoproterenol-treated mice, no variation in the phospholipid/protein ratio was observed. However, the levels of neutral sugars, hexosamines and sialic acid falls drastically in the early prereplicative phase. The decrease in neutral sugars and hexosamines in plasma membranes caused by isoproterenol is imitated by pilocarpine, which induces secretion but little or no increase in DNA synthesis. However, pilocarpine does not mobilize sialic acid from the plasma membrane. Moreover, dosis of isoproterenol that elicits secretion but not mitosis in the acinar cells, does not induce the movement of sialic acid from the plasma membrane. The mobilization of sialic acid from plasma membranes caused by isoproterenol was also demonstrated in an in vitro system. Treatment of the plasma membrane with chloroform/methanol shows that around 60% of the sialic acid is present in the less polar phase. We conclude that the separation of sialic acid from the plasma membrane is one of the early steps in the sequence of events leading to DNA synthesis and cell division in the isoproterenol-stimulated parotid gland of mice.", "contents": "The effect of isoproterenol upon the chemical composition of plasma membranes in the mouse parotid gland. A single intraperitoneal injection of isoproterenol induces resting cells from the acini of the mouse parotid gland to enter the proliferative cycle. Parotid plasma membrane from non-stimulated and isoproterenol-treated mice were prepared by differential centrifugation of the homogenates. Comparing the chemical composition of plasma membranes from non-stimulated and isoproterenol-treated mice, no variation in the phospholipid/protein ratio was observed. However, the levels of neutral sugars, hexosamines and sialic acid falls drastically in the early prereplicative phase. The decrease in neutral sugars and hexosamines in plasma membranes caused by isoproterenol is imitated by pilocarpine, which induces secretion but little or no increase in DNA synthesis. However, pilocarpine does not mobilize sialic acid from the plasma membrane. Moreover, dosis of isoproterenol that elicits secretion but not mitosis in the acinar cells, does not induce the movement of sialic acid from the plasma membrane. The mobilization of sialic acid from plasma membranes caused by isoproterenol was also demonstrated in an in vitro system. Treatment of the plasma membrane with chloroform/methanol shows that around 60% of the sialic acid is present in the less polar phase. We conclude that the separation of sialic acid from the plasma membrane is one of the early steps in the sequence of events leading to DNA synthesis and cell division in the isoproterenol-stimulated parotid gland of mice.", "PMID": 964515}
{"id": "pubmed23n0032_5960", "title": "Thymus-derived striated muscle clones. An ultrastructural analysis of cell differentiation.", "content": "Myogenic clones can be induced in thymus reticulum cultures from adult mice. We investigated the differentiation and maturation of myogenic cells by combined ultrathin section and freeze etching analyses. New insights into the development of cell membranes and cytoplasmic organelles will be discussed in the light of previous investigations of embryonic muscle cell cultures.", "contents": "Thymus-derived striated muscle clones. An ultrastructural analysis of cell differentiation. Myogenic clones can be induced in thymus reticulum cultures from adult mice. We investigated the differentiation and maturation of myogenic cells by combined ultrathin section and freeze etching analyses. New insights into the development of cell membranes and cytoplasmic organelles will be discussed in the light of previous investigations of embryonic muscle cell cultures.", "PMID": 964516}
{"id": "pubmed23n0032_5961", "title": "Mechanisms of cell interaction during primary embryonic induction studied in transfilter experiments.", "content": "The transmission mechanisms operative at different stages of neutralisation during primary embryonic induction of the newt Triturus vulgaris were studied in experiments employing Nuclepore filters placed between interactive tissue explants. The transmission time of the neuralising effect was determined with 0.2 mum Nuclepore filter. In another series of experiments the transformation of neuralised ectoderm by archenteron roof mesoderm into other parts of the CNS was studied. Although sufficiently long induction times were used no transformation into hindbrain structures could be induced across filters with pore sizes from 0.1 mum to 1.0 mum. However, electron microscopy demonstrated cytoplasmic penetration into 0.6 mum filters at 15 h of induction. The results speak against free long-range diffusion of inductive material at the stage of transformation of the neuralised ectoderm to more caudal parts of CSN and warrant a more detailed structural study of the transmission phenomenon in question.", "contents": "Mechanisms of cell interaction during primary embryonic induction studied in transfilter experiments. The transmission mechanisms operative at different stages of neutralisation during primary embryonic induction of the newt Triturus vulgaris were studied in experiments employing Nuclepore filters placed between interactive tissue explants. The transmission time of the neuralising effect was determined with 0.2 mum Nuclepore filter. In another series of experiments the transformation of neuralised ectoderm by archenteron roof mesoderm into other parts of the CNS was studied. Although sufficiently long induction times were used no transformation into hindbrain structures could be induced across filters with pore sizes from 0.1 mum to 1.0 mum. However, electron microscopy demonstrated cytoplasmic penetration into 0.6 mum filters at 15 h of induction. The results speak against free long-range diffusion of inductive material at the stage of transformation of the neuralised ectoderm to more caudal parts of CSN and warrant a more detailed structural study of the transmission phenomenon in question.", "PMID": 964517}
{"id": "pubmed23n0032_5962", "title": "Distribution of acid phosphatase in chick Hensen's node.", "content": "Acid phosphatase distribution and yolk drop ultrastructure in Hensen's node of chick blastoderm at Hamburger-Hamilton stages 3 and 4 are described. At stage 3 large deposits of the reaction product are localised in type-A yolk drops, where signs of intensive degradation are seen. It seems that during this degradation lipid droplets are being formed in the degrading yolk drops. Partly digested yolk drops with a vesicular appearance are extruded from the cells, especially in deeper layers of the node. The phosphatase reaction product is also distributed into intercellular spaces. At stage 4 the cells of the node are more vacuolated and contain acid phosphatase and electron-dense yolk drops in lesser amounts. The possible physiological role of acid phosphatase in Hensen's node during gastrulation is discussed.", "contents": "Distribution of acid phosphatase in chick Hensen's node. Acid phosphatase distribution and yolk drop ultrastructure in Hensen's node of chick blastoderm at Hamburger-Hamilton stages 3 and 4 are described. At stage 3 large deposits of the reaction product are localised in type-A yolk drops, where signs of intensive degradation are seen. It seems that during this degradation lipid droplets are being formed in the degrading yolk drops. Partly digested yolk drops with a vesicular appearance are extruded from the cells, especially in deeper layers of the node. The phosphatase reaction product is also distributed into intercellular spaces. At stage 4 the cells of the node are more vacuolated and contain acid phosphatase and electron-dense yolk drops in lesser amounts. The possible physiological role of acid phosphatase in Hensen's node during gastrulation is discussed.", "PMID": 964518}
{"id": "pubmed23n0032_5963", "title": "Changes in expression of fibroblast surface antigen (SFA) during cytodifferentiation and heterokaryon formation.", "content": "Fibroblast surface antigen (SF antigen, SFA) is a major glycoprotein antigen detected in connective tissue cells (primitive mesenchymal cells, fibroblasts, and astroglial cells). In this study the expression of SFA was followed during differentiation of the mesenchymal cells of the mouse metanephros and during heterokaryon formation produced by Sendai-virus induced fusion of human fibroblasts and chick red blood cells. It was demonstrated by immunofluorescence that SFA was lost from the kidney mesenchymal cells when they differentiate into epithelial cells of the secretory tubuli. During this process SFA became detectable in the basement membrane formed around the tubuli. In cell fusion experiments human SFA which was present as fibrillar network on the surface of cultured fibroblasts, was gradually lost from the heterokaryons when the incorporated chick nuclei became activated. These two sets of experiments indicate that SFA can be used as a phenotypic marker of cytodifferentiation.", "contents": "Changes in expression of fibroblast surface antigen (SFA) during cytodifferentiation and heterokaryon formation. Fibroblast surface antigen (SF antigen, SFA) is a major glycoprotein antigen detected in connective tissue cells (primitive mesenchymal cells, fibroblasts, and astroglial cells). In this study the expression of SFA was followed during differentiation of the mesenchymal cells of the mouse metanephros and during heterokaryon formation produced by Sendai-virus induced fusion of human fibroblasts and chick red blood cells. It was demonstrated by immunofluorescence that SFA was lost from the kidney mesenchymal cells when they differentiate into epithelial cells of the secretory tubuli. During this process SFA became detectable in the basement membrane formed around the tubuli. In cell fusion experiments human SFA which was present as fibrillar network on the surface of cultured fibroblasts, was gradually lost from the heterokaryons when the incorporated chick nuclei became activated. These two sets of experiments indicate that SFA can be used as a phenotypic marker of cytodifferentiation.", "PMID": 964519}
{"id": "pubmed23n0032_5964", "title": "Studies on the organisation of the chicken genome and its expression during myogenesis in vitro.", "content": "DNA from the chicken genome was analysed both by isopycnic centrifugation in cesium salt density gradients and by reassociation analysis using hydroxyapatite (HAP) chromatography. Centrifugation in neutral CsCl revealed a single non-Gaussian band skewed toward the heavy side, but no discrete satellite components. In heavy metal (Ag+ or Hg++)-Cs2SO4 gradients, 4-8 satellite bands were revealed, comprising 5-9% of the total DNA. Purification of the satellites and recentrifugation in neutral CsCl demonstrated that 80-90% of this DNA would band in the shoulder, with the remainder in the main band. These satellites can account at most for 30% of the heavy shoulder DNA, thus most of the heavy shoulder DNA must be of lower repetition frequencies. Reassociation analyses of chicken DNA demonstrated that the complexity of the non-repetitive DNA is 9.49 X 10(8) nucleotide pairs, equivalent to about 90% of the haploid genome. Repetitive DNA comprises only 8-10% of the genome and has the following composition, relative to total DNA: 3.7% intermediate repetitive, 1.9% highly repetitive, and 3.9% \"zero-time binding\" DNA. This unusually low repetitive DNA content may be related to the small genome size of chickens, relative to other vertebrates, and to the presence of many microchromosomes in the chicken karyotype. Total cell RNA extracted from perfusion myoblasts, post-fusion myotubes, and myoblasts grown in BrdU was incubated in large excess with 3H-TdR labelled non-repetitive DNA and the resulting hybrids assayed by HAP chromatography. The amount of non-repetitive DNA represented in the RNA was found to increase from 7-8% in the myoblast stage to 10-11% in myotubes. An even smaller proportion, about 5%, is represented in the RNA of myoblasts prevented from differentiating by growth in BrdU.", "contents": "Studies on the organisation of the chicken genome and its expression during myogenesis in vitro. DNA from the chicken genome was analysed both by isopycnic centrifugation in cesium salt density gradients and by reassociation analysis using hydroxyapatite (HAP) chromatography. Centrifugation in neutral CsCl revealed a single non-Gaussian band skewed toward the heavy side, but no discrete satellite components. In heavy metal (Ag+ or Hg++)-Cs2SO4 gradients, 4-8 satellite bands were revealed, comprising 5-9% of the total DNA. Purification of the satellites and recentrifugation in neutral CsCl demonstrated that 80-90% of this DNA would band in the shoulder, with the remainder in the main band. These satellites can account at most for 30% of the heavy shoulder DNA, thus most of the heavy shoulder DNA must be of lower repetition frequencies. Reassociation analyses of chicken DNA demonstrated that the complexity of the non-repetitive DNA is 9.49 X 10(8) nucleotide pairs, equivalent to about 90% of the haploid genome. Repetitive DNA comprises only 8-10% of the genome and has the following composition, relative to total DNA: 3.7% intermediate repetitive, 1.9% highly repetitive, and 3.9% \"zero-time binding\" DNA. This unusually low repetitive DNA content may be related to the small genome size of chickens, relative to other vertebrates, and to the presence of many microchromosomes in the chicken karyotype. Total cell RNA extracted from perfusion myoblasts, post-fusion myotubes, and myoblasts grown in BrdU was incubated in large excess with 3H-TdR labelled non-repetitive DNA and the resulting hybrids assayed by HAP chromatography. The amount of non-repetitive DNA represented in the RNA was found to increase from 7-8% in the myoblast stage to 10-11% in myotubes. An even smaller proportion, about 5%, is represented in the RNA of myoblasts prevented from differentiating by growth in BrdU.", "PMID": 964520}
{"id": "pubmed23n0032_5965", "title": "Combination effect of citrinin and other chemicals on rat kidney tumorigenesis.", "content": "Histological studies were made on the nephrotoxic effect of citrinin on the kidneys of rats, with or without previous treatment with the nephrotoxic chemicals, N-(3,5-dichlorophenyl)succinimide (NDPS) and N-nitrosodimethylamine (DMN). Oral administration of 0.02% or 0.05% citrinin alone caused signs of kidney injury but did not induce kidney tumors. On treatment with DMN alone, 8 of 14 rats (57.1%) developed kidney tumors; two (14.3%) were renal cell tumors, eight (57.1%) embryonal cell tumors, and one (7.1%) hemangioendothelioma. On the other hand, kidney tumors developed in 18 of 19 rats (94.7%) and 13 of 15 rats (86.7%) by the administration of 0.02% and 0.05% citrinin, respectively, after DMN. The tumors in these two groups were diagnosed histologically as renal cell tumors in 18 (94.7%) in group IV and 13 (86.7%) in group III, and as embryonal cell tumors in 14 (73.7%) in group IV and 9 (60.0%) in group III. Thus, in groups treated with citrinin after DMN the incidence of renal cell tumors was much greater and the incidence of embryonal cell tumors slightly greater than in the group treated with DMN alone. Kidney tumors developed in 4 of 18 rats (22.2%) treated with 0.02% citrinin after NDPS, but treatment with NDPS alone did not induce kidney tumors. Thus, treatment with citrinin changes the histological type and incidence of kidney tumors in rats induced by DMN. Moreover, this study confirms that citrinin in combination with NDPS can induce kidney tumor in rats, which was renal cell tumor (adenoma) histologically.", "contents": "Combination effect of citrinin and other chemicals on rat kidney tumorigenesis. Histological studies were made on the nephrotoxic effect of citrinin on the kidneys of rats, with or without previous treatment with the nephrotoxic chemicals, N-(3,5-dichlorophenyl)succinimide (NDPS) and N-nitrosodimethylamine (DMN). Oral administration of 0.02% or 0.05% citrinin alone caused signs of kidney injury but did not induce kidney tumors. On treatment with DMN alone, 8 of 14 rats (57.1%) developed kidney tumors; two (14.3%) were renal cell tumors, eight (57.1%) embryonal cell tumors, and one (7.1%) hemangioendothelioma. On the other hand, kidney tumors developed in 18 of 19 rats (94.7%) and 13 of 15 rats (86.7%) by the administration of 0.02% and 0.05% citrinin, respectively, after DMN. The tumors in these two groups were diagnosed histologically as renal cell tumors in 18 (94.7%) in group IV and 13 (86.7%) in group III, and as embryonal cell tumors in 14 (73.7%) in group IV and 9 (60.0%) in group III. Thus, in groups treated with citrinin after DMN the incidence of renal cell tumors was much greater and the incidence of embryonal cell tumors slightly greater than in the group treated with DMN alone. Kidney tumors developed in 4 of 18 rats (22.2%) treated with 0.02% citrinin after NDPS, but treatment with NDPS alone did not induce kidney tumors. Thus, treatment with citrinin changes the histological type and incidence of kidney tumors in rats induced by DMN. Moreover, this study confirms that citrinin in combination with NDPS can induce kidney tumor in rats, which was renal cell tumor (adenoma) histologically.", "PMID": 964548}
{"id": "pubmed23n0032_5966", "title": "In vitro transformation of newborn hamster cells induced by sodium nitrite.", "content": "The in vitro carcinogenicity of sodium nitrite was examined. Addition of a high concentration of sodium nitrite (50 mM or 100 mM) to mass cultures of newborn hamster cells for 24 hr resulted in morphological transformation of the cells. The shortest time required for morphological transformation after this treatment was 21 days. Two of 5 transformed cultures produced progressively growing tumors when injected into young adult hamsters. These tumors were diagnosed as fibrosarcomas. In two control cultures of the same experimental groups, transformed cells appeared 10 weeks or more later than those in treated cultures. The chromosomes were analysed at several stages of transformation. In the early stages of transformation, chromosome number varied widely from near diploid to tetraploid, but later they became near diploid. Morphological alteration caused by sodium nitrite was also seen in a short-term assay, in which hamster embryonic cells (1 x 10(4) cells/60 mm dish) were treated and morphology was observed 8 days after the treatment.", "contents": "In vitro transformation of newborn hamster cells induced by sodium nitrite. The in vitro carcinogenicity of sodium nitrite was examined. Addition of a high concentration of sodium nitrite (50 mM or 100 mM) to mass cultures of newborn hamster cells for 24 hr resulted in morphological transformation of the cells. The shortest time required for morphological transformation after this treatment was 21 days. Two of 5 transformed cultures produced progressively growing tumors when injected into young adult hamsters. These tumors were diagnosed as fibrosarcomas. In two control cultures of the same experimental groups, transformed cells appeared 10 weeks or more later than those in treated cultures. The chromosomes were analysed at several stages of transformation. In the early stages of transformation, chromosome number varied widely from near diploid to tetraploid, but later they became near diploid. Morphological alteration caused by sodium nitrite was also seen in a short-term assay, in which hamster embryonic cells (1 x 10(4) cells/60 mm dish) were treated and morphology was observed 8 days after the treatment.", "PMID": 964549}
{"id": "pubmed23n0032_5967", "title": "Different susceptibilities of the urinary bladder epithelium of animal species to three nitroso compounds.", "content": "Differences in the susceptibilities of the urinary bladder epithelium of Wistar rats, ICR mice, Syrian golden hamsters, and Hartley guinea pigs to three N-nitroso compounds, N-butyl-N-(4-hydroxybutyl)nitrosamine, N-ethyl-N-(4-hydroxybutyl)nitrosamine, and N-butyl-N-(3-carboxypropyl)nitrosamine, were examined histologically. The urinary bladder epithelium of rats was the most susceptible to all three compounds, and especially to N-ethyl-N-(4-hydroxybutyl)nitrosamine, and bladder cancers developed in all the rats given this compound. The bladder epithelium of mice was less susceptible than that of rats but, although the incidence of cancer was lower than in rats, that of cancer with invasion was higher. Hamsters were far less susceptible than mice, and cancer developed only in 3 of 41 animals given N-ethyl-N-(4-hydroxybutyl)nitrosamine. Guinea pigs were the least susceptible of the 4 species and no tumors were found. Hisotlogically, most of the tumors induced in rats and mice were transtitional cell carcinomas. The incidence of undifferentiated carcinoma was higher in mice than in rats and cellular or structural atypism of the cancer was also greater in mice. Cancers induced in hamsters were all transitional cell carcinomas showing invasion.", "contents": "Different susceptibilities of the urinary bladder epithelium of animal species to three nitroso compounds. Differences in the susceptibilities of the urinary bladder epithelium of Wistar rats, ICR mice, Syrian golden hamsters, and Hartley guinea pigs to three N-nitroso compounds, N-butyl-N-(4-hydroxybutyl)nitrosamine, N-ethyl-N-(4-hydroxybutyl)nitrosamine, and N-butyl-N-(3-carboxypropyl)nitrosamine, were examined histologically. The urinary bladder epithelium of rats was the most susceptible to all three compounds, and especially to N-ethyl-N-(4-hydroxybutyl)nitrosamine, and bladder cancers developed in all the rats given this compound. The bladder epithelium of mice was less susceptible than that of rats but, although the incidence of cancer was lower than in rats, that of cancer with invasion was higher. Hamsters were far less susceptible than mice, and cancer developed only in 3 of 41 animals given N-ethyl-N-(4-hydroxybutyl)nitrosamine. Guinea pigs were the least susceptible of the 4 species and no tumors were found. Hisotlogically, most of the tumors induced in rats and mice were transtitional cell carcinomas. The incidence of undifferentiated carcinoma was higher in mice than in rats and cellular or structural atypism of the cancer was also greater in mice. Cancers induced in hamsters were all transitional cell carcinomas showing invasion.", "PMID": 964550}
{"id": "pubmed23n0032_5968", "title": "Antitumor activity of degraded products of lentinan: its correlation with molecular weight.", "content": "Lentinan, an antitumor polysaccharide from Lentinus edodes, was degraded to seven fractions by treatment with formic acid. The low molecular-weight fractions (I and II) showed no antitumor activity against sarcoma-180 solid-type tumor and the absorption maximum of Congo Red did not shift in their presence in 0.1M sodium hydroxide. The medium molecular-weight fraction III, which required the increase of doses (5 or 10 mg/kg) for inhibition of tumor growth, caused a little shift. On the other hand, the absorption maximum of Congo Red shifted largely by the presence of high molecular-weight fractions (IV approximately VII) which showed the inhibition ratio of over 95% in a dose of 1 mg/kg. Participation of molecular weight in the antitumor activity of polysaccharides which contain (1 leads to 3)-beta-D-glucan main chain was discussed.", "contents": "Antitumor activity of degraded products of lentinan: its correlation with molecular weight. Lentinan, an antitumor polysaccharide from Lentinus edodes, was degraded to seven fractions by treatment with formic acid. The low molecular-weight fractions (I and II) showed no antitumor activity against sarcoma-180 solid-type tumor and the absorption maximum of Congo Red did not shift in their presence in 0.1M sodium hydroxide. The medium molecular-weight fraction III, which required the increase of doses (5 or 10 mg/kg) for inhibition of tumor growth, caused a little shift. On the other hand, the absorption maximum of Congo Red shifted largely by the presence of high molecular-weight fractions (IV approximately VII) which showed the inhibition ratio of over 95% in a dose of 1 mg/kg. Participation of molecular weight in the antitumor activity of polysaccharides which contain (1 leads to 3)-beta-D-glucan main chain was discussed.", "PMID": 964551}
{"id": "pubmed23n0032_5969", "title": "Effect of plastic bead on gastric carcinogenesis in rats treated with N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "Experiments were made on induction of cancer of the glandular stomach of rats by a combination of oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and insertion of a plastic bead into the gastric lumen. The incidence of cancer with this combination of treatments was greater than by administration of MNNG alone. Fluoroscopic examination showed that barium sulfate remained in the stomach longer when a bead had been inserted into the gastric lumen. This indicates that after insertion of a bead, MNNG must have remained in the stomach longer, and so the period of exposure of the gastric mucosa to the carcinogen must have increased.", "contents": "Effect of plastic bead on gastric carcinogenesis in rats treated with N-methyl-N'-nitro-N-nitrosoguanidine. Experiments were made on induction of cancer of the glandular stomach of rats by a combination of oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and insertion of a plastic bead into the gastric lumen. The incidence of cancer with this combination of treatments was greater than by administration of MNNG alone. Fluoroscopic examination showed that barium sulfate remained in the stomach longer when a bead had been inserted into the gastric lumen. This indicates that after insertion of a bead, MNNG must have remained in the stomach longer, and so the period of exposure of the gastric mucosa to the carcinogen must have increased.", "PMID": 964552}
{"id": "pubmed23n0032_5970", "title": "Effect of drug combination of antitumor activity and myelotoxicity.", "content": "Relationship between the survival time of L-1210-bearing mice and myelosuppression of normal mice after combination chemotherapy was studied. In a variety of combinations of 6-mercaptopurine, 6-thioguanine, and cyclophosphamide, a combination of cyclophosphamide and 6-thioguanine produced the highest increase in life span (ILS) and the highest number of 60-day survivors of all. In contrast, a combination of 6-mercaptopurine and 6-thioguanine showed as low as ILS as did single agents. A combination of 6-mercaptopurine, cyclophosphamide, and 6-thioguanine exhibited the second best effect on survival time of leukemic mice. However, in myelosuppression as measured by changes in the total number of nucleated cells, in the number of hematopoietic colony-forming cell, and in peroxidase level of femoral bone-marrow, the combination of 6-mercaptopurine and 6-thioguanine showed no more toxicity than the other 2-drug combinations. In addition to these findings, the presence of a striking difference in the cell number in ascitic fluid of leukemic mice among the animals given 6-thioguanine, and those given other 2- or 3-drug combinations may suggest that the difference in the antileukemic activity is not due to the difference in the toxicity against the host, but due to the difference in the activity of direct action of combined drugs on leukemic cells in the peritoneal cavity or in other sites.", "contents": "Effect of drug combination of antitumor activity and myelotoxicity. Relationship between the survival time of L-1210-bearing mice and myelosuppression of normal mice after combination chemotherapy was studied. In a variety of combinations of 6-mercaptopurine, 6-thioguanine, and cyclophosphamide, a combination of cyclophosphamide and 6-thioguanine produced the highest increase in life span (ILS) and the highest number of 60-day survivors of all. In contrast, a combination of 6-mercaptopurine and 6-thioguanine showed as low as ILS as did single agents. A combination of 6-mercaptopurine, cyclophosphamide, and 6-thioguanine exhibited the second best effect on survival time of leukemic mice. However, in myelosuppression as measured by changes in the total number of nucleated cells, in the number of hematopoietic colony-forming cell, and in peroxidase level of femoral bone-marrow, the combination of 6-mercaptopurine and 6-thioguanine showed no more toxicity than the other 2-drug combinations. In addition to these findings, the presence of a striking difference in the cell number in ascitic fluid of leukemic mice among the animals given 6-thioguanine, and those given other 2- or 3-drug combinations may suggest that the difference in the antileukemic activity is not due to the difference in the toxicity against the host, but due to the difference in the activity of direct action of combined drugs on leukemic cells in the peritoneal cavity or in other sites.", "PMID": 964553}
{"id": "pubmed23n0032_5971", "title": "Protective effect of mucin on experimental gastric cancer induced by N-methyl-N'-nitro-N-nitrosoguanidine plus sodium chloride in rats.", "content": "Studies were made on the effect of mucin on the induction of gastric carcinomas by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), with or without sodium chloride, in male Wistar rats. Seven groups of rats were treated as follows: Group 4 was given continuously 50 mg MNNG/liter solution to drink and 1 ml of saturated sodium chloride once a week and fed on stock diet supplemented with 4% mucin. Group 2 was given 50 mg MNNG/liter solution and fed on stock diet supplemented with 4% mucin. Group 3 received 1 ml of saturated sodium chloride once a week and 50 mg MNNG/liter solution to drink. Group 1 was treated with MNNG only. Group 5 was fed on stock diet supplemented with 4% mucin. Group 6 was given sodium chloride only. Group 7 was untreated. The incidence of gastric cancer in Group 3 was significantly higher than that in Group 4 (P less than 0.05) or in Group 1 (P less than 0.05). The difference in the incidence of gastric cancer in Groups 2 and 4, and of intestinal tumors in Groups 1 to 4 were not statistically significant. No malignant tumors were seen in Groups 5, 6, and 7. Thus mucin reduced the high incidence of gastric cancer induced by MNNG and sodium chloride to the level induced by MNNG alone, but it had no effect on the incidence of intestinal tumors. The effect of mucin in preventing destruction of the gastric mucosal barrier by sodium chloride and so reducing the induction of gastric cancer is discussed.", "contents": "Protective effect of mucin on experimental gastric cancer induced by N-methyl-N'-nitro-N-nitrosoguanidine plus sodium chloride in rats. Studies were made on the effect of mucin on the induction of gastric carcinomas by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), with or without sodium chloride, in male Wistar rats. Seven groups of rats were treated as follows: Group 4 was given continuously 50 mg MNNG/liter solution to drink and 1 ml of saturated sodium chloride once a week and fed on stock diet supplemented with 4% mucin. Group 2 was given 50 mg MNNG/liter solution and fed on stock diet supplemented with 4% mucin. Group 3 received 1 ml of saturated sodium chloride once a week and 50 mg MNNG/liter solution to drink. Group 1 was treated with MNNG only. Group 5 was fed on stock diet supplemented with 4% mucin. Group 6 was given sodium chloride only. Group 7 was untreated. The incidence of gastric cancer in Group 3 was significantly higher than that in Group 4 (P less than 0.05) or in Group 1 (P less than 0.05). The difference in the incidence of gastric cancer in Groups 2 and 4, and of intestinal tumors in Groups 1 to 4 were not statistically significant. No malignant tumors were seen in Groups 5, 6, and 7. Thus mucin reduced the high incidence of gastric cancer induced by MNNG and sodium chloride to the level induced by MNNG alone, but it had no effect on the incidence of intestinal tumors. The effect of mucin in preventing destruction of the gastric mucosal barrier by sodium chloride and so reducing the induction of gastric cancer is discussed.", "PMID": 964554}
{"id": "pubmed23n0032_5972", "title": "Carcinogenic effect of N-butyl-N-nitrosourethan on CDF1 mice.", "content": "Studies were made on the induction of tumors in mice by N-butyl-N-nitro-sourethan (BNUR), with the following results. 1) Oral administration of 0.04% BNUR in deionized water to CDF1 mice for 20 weeks produced papillomas in the esophagus and forestomach of all 58 mice, and 24 of the mice had squamous cell carcinoma. 2) No skin tumors developed when 0.1%, 0.5%, or 1% BNUR in acetone was painted on the skin on the back of CDF1 mice three times a week for 25 weeks. 3) No sarcomas developed within 30 weeks when a dose of 10, 60, or 120 mg/ml of BNUR in dimethyl sulfoxide solution was injected subcutaneously into the back of CDF1 mice, 10 times at 1-week intervals. 4) In the in vitro malignant transformation test, golden hamster lung fibroblast cells did not show malignant transformation during an observation period of 150 days after treatment with BNUR at a final concentration of 30 and 60 mug/ml for 24 hr. The carcinogenicity and possible mode of action of BNUR are discussed on the basis of these results.", "contents": "Carcinogenic effect of N-butyl-N-nitrosourethan on CDF1 mice. Studies were made on the induction of tumors in mice by N-butyl-N-nitro-sourethan (BNUR), with the following results. 1) Oral administration of 0.04% BNUR in deionized water to CDF1 mice for 20 weeks produced papillomas in the esophagus and forestomach of all 58 mice, and 24 of the mice had squamous cell carcinoma. 2) No skin tumors developed when 0.1%, 0.5%, or 1% BNUR in acetone was painted on the skin on the back of CDF1 mice three times a week for 25 weeks. 3) No sarcomas developed within 30 weeks when a dose of 10, 60, or 120 mg/ml of BNUR in dimethyl sulfoxide solution was injected subcutaneously into the back of CDF1 mice, 10 times at 1-week intervals. 4) In the in vitro malignant transformation test, golden hamster lung fibroblast cells did not show malignant transformation during an observation period of 150 days after treatment with BNUR at a final concentration of 30 and 60 mug/ml for 24 hr. The carcinogenicity and possible mode of action of BNUR are discussed on the basis of these results.", "PMID": 964555}
{"id": "pubmed23n0032_5973", "title": "Leiomyosarcomas of the small intestine induced in dogs by N-methul-N'-nitro-N-nitrosoguanidine.", "content": "Leiomyosarcomas of the small intestine were found in dogs during experimental induction of gastric carcinoma by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Leiomyosarcomas were found most frequenly in the duodenum and jejunum, and occasionally in the stomach but never in the ileum, colon, or rectum. The leiomyosarcomas developed in all the dogs given 50 mug/ml of MNNG in deionized water to drink but not in dogs fed on porridge food made from standard pellet diet mashed with MNNG at the same concentration in tap water. Intestinal sarcomas developed in 3 months to 5 years after the end of MNNG administration, and frequently metastasized to the liver and/or the peritoneum.", "contents": "Leiomyosarcomas of the small intestine induced in dogs by N-methul-N'-nitro-N-nitrosoguanidine. Leiomyosarcomas of the small intestine were found in dogs during experimental induction of gastric carcinoma by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Leiomyosarcomas were found most frequenly in the duodenum and jejunum, and occasionally in the stomach but never in the ileum, colon, or rectum. The leiomyosarcomas developed in all the dogs given 50 mug/ml of MNNG in deionized water to drink but not in dogs fed on porridge food made from standard pellet diet mashed with MNNG at the same concentration in tap water. Intestinal sarcomas developed in 3 months to 5 years after the end of MNNG administration, and frequently metastasized to the liver and/or the peritoneum.", "PMID": 964556}
{"id": "pubmed23n0032_5974", "title": "Mechanism of suppressive effect of basic cupric acetate on rat liver carcinogenesis by ethionine.", "content": "The rate of ethylation of t-RNA in vivo by L-ethionine[ethyl-1-14C] markedly lowered in the liver of rats fed a diet containing copper than that of control. In corporation of the labeled compound into the t-RNA of liver by a single injection. of L-ethionine[ethyl-1-14C] was inhibited as much as 50% by the preceding concurrent administration of copper and ethionine added in the diet to rats. Direct interaction of copper ion with ethylation by ethione in the specific t-RNA of the rat liver was examined as an important biochemical explanation in molecular level for the inhibitory mechanism of copper on rat liver carcinogenesis by ethionine. Profiles of normal t-RNA on methylated albumin-kieselguhr column chromatography revealed three components for leucine, two of which were found at the tube number 40 to 50 and disappeared in the leucyl t-RNA treated with ethionine. The components for leucine that disappeared were normalized in the liver of rats by the concurrent administration of copper.", "contents": "Mechanism of suppressive effect of basic cupric acetate on rat liver carcinogenesis by ethionine. The rate of ethylation of t-RNA in vivo by L-ethionine[ethyl-1-14C] markedly lowered in the liver of rats fed a diet containing copper than that of control. In corporation of the labeled compound into the t-RNA of liver by a single injection. of L-ethionine[ethyl-1-14C] was inhibited as much as 50% by the preceding concurrent administration of copper and ethionine added in the diet to rats. Direct interaction of copper ion with ethylation by ethione in the specific t-RNA of the rat liver was examined as an important biochemical explanation in molecular level for the inhibitory mechanism of copper on rat liver carcinogenesis by ethionine. Profiles of normal t-RNA on methylated albumin-kieselguhr column chromatography revealed three components for leucine, two of which were found at the tube number 40 to 50 and disappeared in the leucyl t-RNA treated with ethionine. The components for leucine that disappeared were normalized in the liver of rats by the concurrent administration of copper.", "PMID": 964557}
{"id": "pubmed23n0032_5975", "title": "Complete regression of Ehrlich solid tumors by the combined administration of glucose, wheat straw hemicellulose-B, and ovoglycopeptide.", "content": "Glucose solution containing wheat straw hemicellulose-B and ovoglycopeptide (W-O-G) has no effect on Ehrlich ascites tumors but completely regresses Ehrlich solid tumors. The carcinostatic effect of this mixture is lost or decreases markedly whenever one of the three substances is lacking. For this glucose solution to possess a carcinostatic effect it is necessary that this hemicellulose-B of W-O-G is the hydrolysate of the extract with 20% NaOH from the residue obtained after extraction of wheat straw with 10% NaOH.", "contents": "Complete regression of Ehrlich solid tumors by the combined administration of glucose, wheat straw hemicellulose-B, and ovoglycopeptide. Glucose solution containing wheat straw hemicellulose-B and ovoglycopeptide (W-O-G) has no effect on Ehrlich ascites tumors but completely regresses Ehrlich solid tumors. The carcinostatic effect of this mixture is lost or decreases markedly whenever one of the three substances is lacking. For this glucose solution to possess a carcinostatic effect it is necessary that this hemicellulose-B of W-O-G is the hydrolysate of the extract with 20% NaOH from the residue obtained after extraction of wheat straw with 10% NaOH.", "PMID": 964558}
{"id": "pubmed23n0032_5976", "title": "Induction of transplantation immunity by dansylated tumor cells.", "content": "Tumor cells were coupled with fluorecent dansyl group in aqueous medium by dansyl chloride-cycloheptaamylose complex (CDC) without destruction of the cells. C57BL/6 mice and Donryu rats pretreated respectively with dansylated EL4 leukemia cells and with dansylated Yoshida sarcoma cells acquired transplantation immunity to the corresponding tumor cells. Serum and spleen cells obtained from EL4 immune mice showed cytotoxicity to EL4 cells but not to other allogeneic leukemia cells. Hapten-specific cytotoxicity of immune serum and spleen cells was not observed in the present immune system.", "contents": "Induction of transplantation immunity by dansylated tumor cells. Tumor cells were coupled with fluorecent dansyl group in aqueous medium by dansyl chloride-cycloheptaamylose complex (CDC) without destruction of the cells. C57BL/6 mice and Donryu rats pretreated respectively with dansylated EL4 leukemia cells and with dansylated Yoshida sarcoma cells acquired transplantation immunity to the corresponding tumor cells. Serum and spleen cells obtained from EL4 immune mice showed cytotoxicity to EL4 cells but not to other allogeneic leukemia cells. Hapten-specific cytotoxicity of immune serum and spleen cells was not observed in the present immune system.", "PMID": 964559}
{"id": "pubmed23n0032_5977", "title": "Antitumor activity of berberrubine derivatives.", "content": "The antitumor activity of berberine, berberrubine, and their derivatives against sarcoma-180 ascites was determined by the total packed cell volume method. Berbine and tetrahydroberberine derivatives had no antitumor activity, but berberrubine (9-demethylberberine) and the ester derivatives of berberrubine had a strong antitumor activity. ED90 of berberrubine, its acetate and benzoate, were 15, 23, and 44 mg/kg, respectively. The therapeutic indices (LD10/ED90 by the present method) of these compounds were as follows: Berberrubine hydrochloride, 6.7 approximately 9.4; 9-acetyl-9-demethylberberine (9-acetylberberrubine) chloride, 7.6 approximately 8.7; 9-benzoyl-9-demethylberberine (9-benzoylberberrubine) chloride, 3.4 approximately 4.9.", "contents": "Antitumor activity of berberrubine derivatives. The antitumor activity of berberine, berberrubine, and their derivatives against sarcoma-180 ascites was determined by the total packed cell volume method. Berbine and tetrahydroberberine derivatives had no antitumor activity, but berberrubine (9-demethylberberine) and the ester derivatives of berberrubine had a strong antitumor activity. ED90 of berberrubine, its acetate and benzoate, were 15, 23, and 44 mg/kg, respectively. The therapeutic indices (LD10/ED90 by the present method) of these compounds were as follows: Berberrubine hydrochloride, 6.7 approximately 9.4; 9-acetyl-9-demethylberberine (9-acetylberberrubine) chloride, 7.6 approximately 8.7; 9-benzoyl-9-demethylberberine (9-benzoylberberrubine) chloride, 3.4 approximately 4.9.", "PMID": 964560}
{"id": "pubmed23n0032_5978", "title": "Combined effect of cyclocytidine and lentinan of spontaneous mammary tumors in mice.", "content": "Cytotoxic cytidine analog, cyclocytidine, and antitumor immunopotentiator, lentinan, offered an opportunity of testing the effect of combined modality of cytocidal agent and immunopotentiator on spontaneous mammary tumors of mice. Lentinan did not improve the therapeutic effect of cyclocytidine; it only prevented the early toxic death of mice due to cyclocytidine in one strain of mice but not in the other strain.", "contents": "Combined effect of cyclocytidine and lentinan of spontaneous mammary tumors in mice. Cytotoxic cytidine analog, cyclocytidine, and antitumor immunopotentiator, lentinan, offered an opportunity of testing the effect of combined modality of cytocidal agent and immunopotentiator on spontaneous mammary tumors of mice. Lentinan did not improve the therapeutic effect of cyclocytidine; it only prevented the early toxic death of mice due to cyclocytidine in one strain of mice but not in the other strain.", "PMID": 964561}
{"id": "pubmed23n0032_5979", "title": "Colonic perforation in Crohn's disease.", "content": "Three cases of acute, free perforation of the colon in Crohn's disease are described and their clinical and pathological features are detailed. Colonic perforation in Crohn's disease bears no definite relationship to obstruction or adrenocorticosteroid therapy. It may occur with or without toxic megacolon, and pathological study has not thus far suggested a common pathogenetic mechanism. Although free perforation has been considered to be extremely rare in Crohn's colitis, it is likely that this complication is more common than previously believed.", "contents": "Colonic perforation in Crohn's disease. Three cases of acute, free perforation of the colon in Crohn's disease are described and their clinical and pathological features are detailed. Colonic perforation in Crohn's disease bears no definite relationship to obstruction or adrenocorticosteroid therapy. It may occur with or without toxic megacolon, and pathological study has not thus far suggested a common pathogenetic mechanism. Although free perforation has been considered to be extremely rare in Crohn's colitis, it is likely that this complication is more common than previously believed.", "PMID": 964562}
{"id": "pubmed23n0032_5980", "title": "Candida esophagitis: a prospective study of 27 cases.", "content": "A prospective study of candida esophagitis was undertaken to determine the spectrum of this disease in a general hospital. During 1 year, in 370 consecutive endoscopies, 27 patients with Candida esophagitis were detected. The diagnosis was established by finding white plaques on endoscopy, yeast organisms on microscopic examination of a direct smear from the plaques, and a serum agglutinin titer of at least 1:160. Of these 27 patients, 14 had esophageal symptoms. Twelve patients were reendoscoped after nystatin or nystatin and flucytosine therapy. Nine patients showed absence of lesions, a negative smear, and disappearance of symptoms. Control patients had no plaques on endoscopy, no yeast organisms on microscopical examination of esophageal brushings, and a positive titer in 4 to 17% of cases. A minimal agglutinin titer of 1:160 was found in 4 to 12% of two additional groups on controls. Absence of titer precluded a diagnosis of Candida esophagitis.", "contents": "Candida esophagitis: a prospective study of 27 cases. A prospective study of candida esophagitis was undertaken to determine the spectrum of this disease in a general hospital. During 1 year, in 370 consecutive endoscopies, 27 patients with Candida esophagitis were detected. The diagnosis was established by finding white plaques on endoscopy, yeast organisms on microscopic examination of a direct smear from the plaques, and a serum agglutinin titer of at least 1:160. Of these 27 patients, 14 had esophageal symptoms. Twelve patients were reendoscoped after nystatin or nystatin and flucytosine therapy. Nine patients showed absence of lesions, a negative smear, and disappearance of symptoms. Control patients had no plaques on endoscopy, no yeast organisms on microscopical examination of esophageal brushings, and a positive titer in 4 to 17% of cases. A minimal agglutinin titer of 1:160 was found in 4 to 12% of two additional groups on controls. Absence of titer precluded a diagnosis of Candida esophagitis.", "PMID": 964563}
{"id": "pubmed23n0032_5981", "title": "Antral gastrin concentration in patients with vagotomy and pyloroplasty.", "content": "Antral gastrin concentration was measured in endoscopic forceps biopsy specimens and prepyloric mucosa in patients with vagotomy and pyloroplasty, duodenal ulcer patients and nonulcer patients. Antral gastrin concentration in vagotomy and pyloroplasty patients, 35.5 +/- 4.1 ng of gastrin per mg of tissue (mean +/- SEM), was significantly greater (P less than 0.01) than antral gastrin concentration in duodenal ulcer patients, 13.0 +/- 2.4 ng per mg, and antral gastrin concentration in controls, 14.8 +/- 3.1 ng per mg. Basal and meal-stimulated serum gastrin levels in vagotomy and pyloroplasty patients were also significantly greater (P less than 0.05) than gastrin levels in either duodenal ulcer patients or controls. Acid secretion rates were not significantly correlated with serum or antral gastrin concentrations in the patients groups.", "contents": "Antral gastrin concentration in patients with vagotomy and pyloroplasty. Antral gastrin concentration was measured in endoscopic forceps biopsy specimens and prepyloric mucosa in patients with vagotomy and pyloroplasty, duodenal ulcer patients and nonulcer patients. Antral gastrin concentration in vagotomy and pyloroplasty patients, 35.5 +/- 4.1 ng of gastrin per mg of tissue (mean +/- SEM), was significantly greater (P less than 0.01) than antral gastrin concentration in duodenal ulcer patients, 13.0 +/- 2.4 ng per mg, and antral gastrin concentration in controls, 14.8 +/- 3.1 ng per mg. Basal and meal-stimulated serum gastrin levels in vagotomy and pyloroplasty patients were also significantly greater (P less than 0.05) than gastrin levels in either duodenal ulcer patients or controls. Acid secretion rates were not significantly correlated with serum or antral gastrin concentrations in the patients groups.", "PMID": 964564}
{"id": "pubmed23n0032_5982", "title": "Absorptive and digestive function of the jejunum after jejunoileal bypass for treatment of human obesity.", "content": "Rates of absorption of leucine, glycylleucine, and glucose, and rates of hydrolysis of maltose were determined in the jejunum of a group of obese persons before and at intervals (between 2 and 20 montsh) after jejunoileal bypass for the treatment of obesity. The leucine absorption rate was significantly reduced after the bypass, but the absorption rates of glycylleucine and glucose as well as the hydrolysis rate of maltose were unchanged. Light microscopic investigation of the jejunal mucosa, obtained by a peroral biopsy technique before and at 7 months after by bypass operation, did not reveal any change in the histological appearance of this tissue. The plasma aminograms of all 7 patients were compared before and at intervals after the bypass operation; all exhibited a constant pattern of change that was characterized by significant decreases in the concentrations of serine and glycine and by significant decreases in the concentrations of valine, isoleucine, leucine, tyrosine, and phenylalanine. This pattern of change, which is characteristic of protein depletion, persisted during the entire period of observation. Two of these 7 patients developed laboratory evidence of hepatic dysfunction. It is concluded that (1) protein depletion is common to all patients with jejunoileal bypass with or without hepatic dysfunction; and (2) protein depletion results in a sustained reduction in free amino acid absorption in the jejunum.", "contents": "Absorptive and digestive function of the jejunum after jejunoileal bypass for treatment of human obesity. Rates of absorption of leucine, glycylleucine, and glucose, and rates of hydrolysis of maltose were determined in the jejunum of a group of obese persons before and at intervals (between 2 and 20 montsh) after jejunoileal bypass for the treatment of obesity. The leucine absorption rate was significantly reduced after the bypass, but the absorption rates of glycylleucine and glucose as well as the hydrolysis rate of maltose were unchanged. Light microscopic investigation of the jejunal mucosa, obtained by a peroral biopsy technique before and at 7 months after by bypass operation, did not reveal any change in the histological appearance of this tissue. The plasma aminograms of all 7 patients were compared before and at intervals after the bypass operation; all exhibited a constant pattern of change that was characterized by significant decreases in the concentrations of serine and glycine and by significant decreases in the concentrations of valine, isoleucine, leucine, tyrosine, and phenylalanine. This pattern of change, which is characteristic of protein depletion, persisted during the entire period of observation. Two of these 7 patients developed laboratory evidence of hepatic dysfunction. It is concluded that (1) protein depletion is common to all patients with jejunoileal bypass with or without hepatic dysfunction; and (2) protein depletion results in a sustained reduction in free amino acid absorption in the jejunum.", "PMID": 964565}
{"id": "pubmed23n0032_5983", "title": "Medical and surgical therapy in diverticular disease: a comparative study.", "content": "The course of 132 patients with documented acute diverticulitis was analyzed: 99 patients treated medically and 33 patients treated surgically were followed for an average of 9.2 years. Seventy-three per cent of the medical group and 79% of the surgical group had no further symptoms or hospital admissions as a result of their diverticular disease once they were recovered from the acute episode. For three-quarters of the patients, therefore, acute diverticulitis occurred as a single episode that responded to either medical or surgical management. Considering the morbidity and cost to the patient, the treatment of the patient recovered from acute diverticulitis should be medical, with operation reserved for complications.", "contents": "Medical and surgical therapy in diverticular disease: a comparative study. The course of 132 patients with documented acute diverticulitis was analyzed: 99 patients treated medically and 33 patients treated surgically were followed for an average of 9.2 years. Seventy-three per cent of the medical group and 79% of the surgical group had no further symptoms or hospital admissions as a result of their diverticular disease once they were recovered from the acute episode. For three-quarters of the patients, therefore, acute diverticulitis occurred as a single episode that responded to either medical or surgical management. Considering the morbidity and cost to the patient, the treatment of the patient recovered from acute diverticulitis should be medical, with operation reserved for complications.", "PMID": 964566}
{"id": "pubmed23n0032_5984", "title": "Effect of caffeine on the human small intestine.", "content": "Methylxanthines produce intracellular accumulation of cyclic 3'5'-AMP (cAMP) by inhibition of phosphodiesterase and mucosal cAMP accumulation. Cyclic AMP is thought to mediate small intestinal secretion caused by some enterotoxins, hormones, and methylxanthines. These studies were designed to evaluate the effect of caffeine on small intestinal net fluid movement and transit times. The administration of caffeine in amounts ordinarily contained in many beverages and medications (75 to 300 mg) resulted in striking net secretion in the jejunum which lasted at least 15 minutes. This occurred in six of seven studies. Baseline net absorption of 0.5 ml per cm per hr was reversed to net secretion of 6.0 +/- 2.2 ml per cm per hr after oral caffeine ingestion (P less than 0.01). Net secretion also occurred in the ileum in seven of eight studies, but the onset of secretion appeared 35 min later than in the jejunum. These patterns of secretion correlated best with the passage of the intestinal bolus of caffeine rather than plasma caffeine levels. In contrast to other net secretory conditions, which increase the speed of transit, small intestinal transit times, as determined by dye dilution methods, were unchanged by caffeine. It is possible that methylxanthine-induced small intestinal secretion may play a role in the symptoms experienced by some patients with functional diarrhea.", "contents": "Effect of caffeine on the human small intestine. Methylxanthines produce intracellular accumulation of cyclic 3'5'-AMP (cAMP) by inhibition of phosphodiesterase and mucosal cAMP accumulation. Cyclic AMP is thought to mediate small intestinal secretion caused by some enterotoxins, hormones, and methylxanthines. These studies were designed to evaluate the effect of caffeine on small intestinal net fluid movement and transit times. The administration of caffeine in amounts ordinarily contained in many beverages and medications (75 to 300 mg) resulted in striking net secretion in the jejunum which lasted at least 15 minutes. This occurred in six of seven studies. Baseline net absorption of 0.5 ml per cm per hr was reversed to net secretion of 6.0 +/- 2.2 ml per cm per hr after oral caffeine ingestion (P less than 0.01). Net secretion also occurred in the ileum in seven of eight studies, but the onset of secretion appeared 35 min later than in the jejunum. These patterns of secretion correlated best with the passage of the intestinal bolus of caffeine rather than plasma caffeine levels. In contrast to other net secretory conditions, which increase the speed of transit, small intestinal transit times, as determined by dye dilution methods, were unchanged by caffeine. It is possible that methylxanthine-induced small intestinal secretion may play a role in the symptoms experienced by some patients with functional diarrhea.", "PMID": 964567}
{"id": "pubmed23n0032_5985", "title": "Effect of intestinal hormones and peptides on intragastric pressure in dogs.", "content": "Intragastric pressure was measured in dogs with gastric fistulas by using a flaccid balloon containing 500 ml of water. Intravenous infusion of cholecystokinin (20% pure), the carboxyl-terminal octapeptide of cholecystokinin, secretin, and vasoactive intestinal peptide produced dose-related decreases in intragastric pressure with maximal decreases of 40% or more. Glucagon and gastric inhibitory peptide produced smaller decreases in intragastric pressure. Motilin caused a dose-related increase in intragastric pressure that lasted only about 7 min despite continuing infusion of the peptide. The half-dose of cholecystokinin or of octapeptide of cholecystokinin for pancreatic protein secretion and the half-dose of secretin for pancreatic bicarbonate secretion each produced significant inhibition of intragastric pressure, suggesting that these hormones play a physiological reole in regulating gastric pressure.", "contents": "Effect of intestinal hormones and peptides on intragastric pressure in dogs. Intragastric pressure was measured in dogs with gastric fistulas by using a flaccid balloon containing 500 ml of water. Intravenous infusion of cholecystokinin (20% pure), the carboxyl-terminal octapeptide of cholecystokinin, secretin, and vasoactive intestinal peptide produced dose-related decreases in intragastric pressure with maximal decreases of 40% or more. Glucagon and gastric inhibitory peptide produced smaller decreases in intragastric pressure. Motilin caused a dose-related increase in intragastric pressure that lasted only about 7 min despite continuing infusion of the peptide. The half-dose of cholecystokinin or of octapeptide of cholecystokinin for pancreatic protein secretion and the half-dose of secretin for pancreatic bicarbonate secretion each produced significant inhibition of intragastric pressure, suggesting that these hormones play a physiological reole in regulating gastric pressure.", "PMID": 964568}
{"id": "pubmed23n0032_5986", "title": "Maturation and differentiation of cultured fetal stomach. Effects of corticosteroids, pentagastrin, and cytochalasin B.", "content": "Gastric mucosa of fetal rats undergoes striking developmental changes during the last few days of gestation in utero. To investigate some aspects of this process, gastric explants from 18-day fetuses (4 days before birth) were maintained in organ culture for 3 days, then assessed by light and electron microscopy. The epithelia from base line uncultured stomachs were stratified and morphologically undifferentiated. During culture in basic medium (Leibovitz L 15), modest maturation of antral and fundic architecture occurred, characterized by epithelial invagination to produce small pit-glands. Secretory granules appeared in occasional epithelial cells, and cytochemistry indicated that most were mucous granules. Addition of pentagastrin (10(-9) to 2 X 10(-7) M) did not induce further morphological maturation in this system. However, addition of cortisol (10(-6) to 10(-5) M) resulted in a marked, dose-related increase of pit-gland formation and of cytological differentiation (appearance of secretory granules). This cortisol-induced architectural maturation was completely inhibited by the mold metabolite cytochalasin B (10(-5) M). The results indicate that fetal gastric maturation can be partially reproduced by culture in chemically defined media, and also suggest that corticosteroids may plan an important role in gastric organogenesis.", "contents": "Maturation and differentiation of cultured fetal stomach. Effects of corticosteroids, pentagastrin, and cytochalasin B. Gastric mucosa of fetal rats undergoes striking developmental changes during the last few days of gestation in utero. To investigate some aspects of this process, gastric explants from 18-day fetuses (4 days before birth) were maintained in organ culture for 3 days, then assessed by light and electron microscopy. The epithelia from base line uncultured stomachs were stratified and morphologically undifferentiated. During culture in basic medium (Leibovitz L 15), modest maturation of antral and fundic architecture occurred, characterized by epithelial invagination to produce small pit-glands. Secretory granules appeared in occasional epithelial cells, and cytochemistry indicated that most were mucous granules. Addition of pentagastrin (10(-9) to 2 X 10(-7) M) did not induce further morphological maturation in this system. However, addition of cortisol (10(-6) to 10(-5) M) resulted in a marked, dose-related increase of pit-gland formation and of cytological differentiation (appearance of secretory granules). This cortisol-induced architectural maturation was completely inhibited by the mold metabolite cytochalasin B (10(-5) M). The results indicate that fetal gastric maturation can be partially reproduced by culture in chemically defined media, and also suggest that corticosteroids may plan an important role in gastric organogenesis.", "PMID": 964569}
{"id": "pubmed23n0032_5987", "title": "The effect of ischemic villus cell damage on crypt cell proliferation in the small intestine: evidence for a feedback control mechanism.", "content": "In recent years the hypothesis that the number of villus cells regulates crypt cell proliferation in the epithelium of the small intestine has been brought forward by a number of investigators. To test this hypothesis, the villus cell population was reduced by clamping the superior mesenteric artery and vein in rats for 1 hr and the effects on the intestinal epithelium were studied during the first 24 hr. It was shown that temporary interruption of the blood flow to the small intestine led to a marked decrease in the number of functional villus cells within 2 hr; preferentially, cells from the upper part of the villus were lost and the number of crypt cells was not affected. This reduction in the number of villus cells led to an increase in the percentage of labeled crypt cells after pulse labeling with [3H]thymidine, and an expansion of the proliferative cell compartment in the crypt. After a peak of proliferative activity at 16 hr, the investigated crypt cell kinetic parameters approached control values after 24 hr, as did the number of villus cells. The enzyme activities of nonspecific esterase and neutral alpha-glucosidase showed marked decreases in isolated crypt and villus cell compartments as crypt cell proliferation increased. These data support the view that the feedback control of crypt cell proliferation by the functional villus cells, and confirm earlier data on the influence of changing cell kinetics on crypt cell maturation. Additional data which were obtained after creating temporary ischemia in part of the small intestine support the hypothesis of a feedback control of crypt cell proliferation by the functional villus cells, and confirm earlier data on the influence of changing cell kinetics on crypt cell maturation. Additional data which were obtained after creating temporary ischemia in part of the small intestine support the view that the feedback control of proliferation by the villus cells is a local control mechanism.", "contents": "The effect of ischemic villus cell damage on crypt cell proliferation in the small intestine: evidence for a feedback control mechanism. In recent years the hypothesis that the number of villus cells regulates crypt cell proliferation in the epithelium of the small intestine has been brought forward by a number of investigators. To test this hypothesis, the villus cell population was reduced by clamping the superior mesenteric artery and vein in rats for 1 hr and the effects on the intestinal epithelium were studied during the first 24 hr. It was shown that temporary interruption of the blood flow to the small intestine led to a marked decrease in the number of functional villus cells within 2 hr; preferentially, cells from the upper part of the villus were lost and the number of crypt cells was not affected. This reduction in the number of villus cells led to an increase in the percentage of labeled crypt cells after pulse labeling with [3H]thymidine, and an expansion of the proliferative cell compartment in the crypt. After a peak of proliferative activity at 16 hr, the investigated crypt cell kinetic parameters approached control values after 24 hr, as did the number of villus cells. The enzyme activities of nonspecific esterase and neutral alpha-glucosidase showed marked decreases in isolated crypt and villus cell compartments as crypt cell proliferation increased. These data support the view that the feedback control of crypt cell proliferation by the functional villus cells, and confirm earlier data on the influence of changing cell kinetics on crypt cell maturation. Additional data which were obtained after creating temporary ischemia in part of the small intestine support the hypothesis of a feedback control of crypt cell proliferation by the functional villus cells, and confirm earlier data on the influence of changing cell kinetics on crypt cell maturation. Additional data which were obtained after creating temporary ischemia in part of the small intestine support the view that the feedback control of proliferation by the villus cells is a local control mechanism.", "PMID": 964570}
{"id": "pubmed23n0032_5988", "title": "Post-transfusion chronic liver disease.", "content": "To document the sequelae of acute hepatitis among recipients of commercial and volunteer blood and to assess factors influencing the development of chronic hepatitis (CH), 47 patients with post-transfusion hepatitis were followed prospectively from the time they received their transfusions. Twenty-nine had prolongation of at least 2-fold serum glutamic pyruvic transaminase (T) elevations for more than 20 weeks, and were classified as CH. When the patients with CH were compared to those with only acute hepatitis (abnormal T for less that 20 weeks), no difference was found with respect to age, sex, number of units transfused, incubation period, presence or absence of symptoms, occurrence of jaundice, maximum T, receipt or development of hepatitis B surface antigen or antibody, underlying illness, or area of the hospital where the patient was treated. Liver biopsies in 15 of the 29 revealed chronic-active hepatitis in 9, chronic persistent hepatitis in 2, unresolved hepatitis in 4. Five of the 9 patients with chronic active hepatitis were without symptoms. None of these died or have developed cirrhosis. Because chronic liver disease frequently developed after acute post-transfusion hepatitis among multiply transfused hepatitis B surface antigen negative blood recipients, close follow-up, including liver biopsy, is warranted in such patients with prolonged transaminase elevations.", "contents": "Post-transfusion chronic liver disease. To document the sequelae of acute hepatitis among recipients of commercial and volunteer blood and to assess factors influencing the development of chronic hepatitis (CH), 47 patients with post-transfusion hepatitis were followed prospectively from the time they received their transfusions. Twenty-nine had prolongation of at least 2-fold serum glutamic pyruvic transaminase (T) elevations for more than 20 weeks, and were classified as CH. When the patients with CH were compared to those with only acute hepatitis (abnormal T for less that 20 weeks), no difference was found with respect to age, sex, number of units transfused, incubation period, presence or absence of symptoms, occurrence of jaundice, maximum T, receipt or development of hepatitis B surface antigen or antibody, underlying illness, or area of the hospital where the patient was treated. Liver biopsies in 15 of the 29 revealed chronic-active hepatitis in 9, chronic persistent hepatitis in 2, unresolved hepatitis in 4. Five of the 9 patients with chronic active hepatitis were without symptoms. None of these died or have developed cirrhosis. Because chronic liver disease frequently developed after acute post-transfusion hepatitis among multiply transfused hepatitis B surface antigen negative blood recipients, close follow-up, including liver biopsy, is warranted in such patients with prolonged transaminase elevations.", "PMID": 964571}
{"id": "pubmed23n0032_5989", "title": "Kinetics of [14C]cholic acid in fulminant hepatic failure: a prognostic test.", "content": "The kinetics of intravenously injected [14C]cholic acid have been investigated in 14 patients with fulminant hepatic failure, 24 to 36 hr after the development of grade IV encephalopathy. Radioactivity was measured in plasma samples and in the individual plasma bile acid fractions after separation by thin layer chromatography. Plasma disappearance curves of the free [14C]cholic acid were calculated by an iterative nonlinear least squares fitting procedure using a computer. The disappearance of total plasma radioactivity was similar in all patients and greatly prolonged compared with healthy subjects. However, the plasma disappearance of free [14C]cholic acid was significantly faster in the 8 patients who recovered consciousness than in the 6 who did not. Plasma disappearance of free [14C]cholic acid correlated highly significantly with the proportion of conjugated [14C]cholate in plasma. All patients in whom more than 70% of plasma radioactivity was in the conjugated fraction 3 hr after injection survived and left hospital, whereas all of those in whom less than 55% was conjugated died. Measuring the percentage conjugation of [14C]cholate 3 hr after injection may therefore be a useful test of residual liver function in hepatic failure, as a guide to prognosis and in evaluating new forms of treatment.", "contents": "Kinetics of [14C]cholic acid in fulminant hepatic failure: a prognostic test. The kinetics of intravenously injected [14C]cholic acid have been investigated in 14 patients with fulminant hepatic failure, 24 to 36 hr after the development of grade IV encephalopathy. Radioactivity was measured in plasma samples and in the individual plasma bile acid fractions after separation by thin layer chromatography. Plasma disappearance curves of the free [14C]cholic acid were calculated by an iterative nonlinear least squares fitting procedure using a computer. The disappearance of total plasma radioactivity was similar in all patients and greatly prolonged compared with healthy subjects. However, the plasma disappearance of free [14C]cholic acid was significantly faster in the 8 patients who recovered consciousness than in the 6 who did not. Plasma disappearance of free [14C]cholic acid correlated highly significantly with the proportion of conjugated [14C]cholate in plasma. All patients in whom more than 70% of plasma radioactivity was in the conjugated fraction 3 hr after injection survived and left hospital, whereas all of those in whom less than 55% was conjugated died. Measuring the percentage conjugation of [14C]cholate 3 hr after injection may therefore be a useful test of residual liver function in hepatic failure, as a guide to prognosis and in evaluating new forms of treatment.", "PMID": 964572}
{"id": "pubmed23n0032_5990", "title": "Lincomycin-induced cholecystitis and gallstones in guinea pigs.", "content": "Guinea pigs given lincomycin 60 mg per kg per day showed a striking increase in the renewal of epithelial cells in the gallbladder. This was detectable after only 24 hr of treatment. By 48 hr a precipitate consisting predominantly of calcium and bilirubin had formed in the gallbladder. Gallbladder bile glycoprotein concentrations rose progressively. At 8 days epithelial dysplasia was marked. Treatment was discontinued at 9 days. The guinea pigs that survived beyond 34 days showed that new tubuloalveolar glands had formed focally in the body and fundus of the gallbladder. Multiple calcium-containing stones were present. The failure to culture bacteria from bile, the failure of the bile salt pattern to change, and the progressive nature of the epithelial injury suggested that the changes resulted from a direct toxic effect and were not dependent on the antibiotic activity of this drug. It was concluded that damage to the epithelium of the gallbladder preceded the formation of a precipitate and initiated histological changes which culminated, at the time that the precipitate became organized into stones, in impressive glandular metaplasia.", "contents": "Lincomycin-induced cholecystitis and gallstones in guinea pigs. Guinea pigs given lincomycin 60 mg per kg per day showed a striking increase in the renewal of epithelial cells in the gallbladder. This was detectable after only 24 hr of treatment. By 48 hr a precipitate consisting predominantly of calcium and bilirubin had formed in the gallbladder. Gallbladder bile glycoprotein concentrations rose progressively. At 8 days epithelial dysplasia was marked. Treatment was discontinued at 9 days. The guinea pigs that survived beyond 34 days showed that new tubuloalveolar glands had formed focally in the body and fundus of the gallbladder. Multiple calcium-containing stones were present. The failure to culture bacteria from bile, the failure of the bile salt pattern to change, and the progressive nature of the epithelial injury suggested that the changes resulted from a direct toxic effect and were not dependent on the antibiotic activity of this drug. It was concluded that damage to the epithelium of the gallbladder preceded the formation of a precipitate and initiated histological changes which culminated, at the time that the precipitate became organized into stones, in impressive glandular metaplasia.", "PMID": 964573}
{"id": "pubmed23n0032_5991", "title": "Evaluation in dogs of cross-circulation in the treatment of acute hepatic necrosis induced by yellow phosphorus.", "content": "The efficacy of cross-circulation in the treatment of acute liver failure has been evaluated in dogs. Four of 5 dogs administered a dose of yellow phosphorus that is lethal 90% of the time survived after treatment by cross-circulation of whole blood for between 1 and 8 hr with a normal dog. In 2 normal unmatched dogs plasma cross-circulations were performed over a period of 31 days without any clinical or laboratory manifestation of hypersensitivity except for lymphocytotoxic antibody titer rise. The results suggest that whole blood cross-circulation is effective and imply that a single donor could be utilized for prolonged periods of plasma cross-circulation with avoidance of immunological consequences of whole blood exchange.", "contents": "Evaluation in dogs of cross-circulation in the treatment of acute hepatic necrosis induced by yellow phosphorus. The efficacy of cross-circulation in the treatment of acute liver failure has been evaluated in dogs. Four of 5 dogs administered a dose of yellow phosphorus that is lethal 90% of the time survived after treatment by cross-circulation of whole blood for between 1 and 8 hr with a normal dog. In 2 normal unmatched dogs plasma cross-circulations were performed over a period of 31 days without any clinical or laboratory manifestation of hypersensitivity except for lymphocytotoxic antibody titer rise. The results suggest that whole blood cross-circulation is effective and imply that a single donor could be utilized for prolonged periods of plasma cross-circulation with avoidance of immunological consequences of whole blood exchange.", "PMID": 964574}
{"id": "pubmed23n0032_5992", "title": "Radioautographic observations of a 19-month-old ileal conduit.", "content": "The influence of an altered environment of the cellular kinetics and histological appearance of ileal mucosa used as a urinary bladder was investigated in a patient with a conduit of 1 year and 7 months' duration. After injection of 10 mc of 3H-TdR, multiple biopsies were taken spanning a 3-day period. No increase in inflammatory cell numbers was seen and villous atrophy was minimal. A generation time or total cell cycle time of 36 hr was observed, with a G1 phase of 22 hr and an S phase of 11 hr. At 1 hr 2.4 3H-TdR-labeled cells per crypt column and a mitotic index of 1.4% were obtained. The complet labeled mitosis curve obtained on the epithelial cells of the 19-month-old ileal conduit mucosa demonstrated a retention of the kinetics and histological characteristics of normal ileal mucosa, contrary to previously documented findings.", "contents": "Radioautographic observations of a 19-month-old ileal conduit. The influence of an altered environment of the cellular kinetics and histological appearance of ileal mucosa used as a urinary bladder was investigated in a patient with a conduit of 1 year and 7 months' duration. After injection of 10 mc of 3H-TdR, multiple biopsies were taken spanning a 3-day period. No increase in inflammatory cell numbers was seen and villous atrophy was minimal. A generation time or total cell cycle time of 36 hr was observed, with a G1 phase of 22 hr and an S phase of 11 hr. At 1 hr 2.4 3H-TdR-labeled cells per crypt column and a mitotic index of 1.4% were obtained. The complet labeled mitosis curve obtained on the epithelial cells of the 19-month-old ileal conduit mucosa demonstrated a retention of the kinetics and histological characteristics of normal ileal mucosa, contrary to previously documented findings.", "PMID": 964575}
{"id": "pubmed23n0032_5993", "title": "Congenital hepatic fibrosis with dilation of intrahepatic bile ducts. A therapeutic approach.", "content": "A case of congenital hepatic fibrosis with dilation of the intrahepatic ducts is presented. External drainage and lavage of the biliary tract through a prosthetic \"Y\" tube and internal drainage accomplished by a Roux en-Y cholangiojejunostomy resulted in prevention of the patient's recurrent bouts of cholangitis and sepsis. Surgical therapy for recurrent cholangitis in this disease is reviewed.", "contents": "Congenital hepatic fibrosis with dilation of intrahepatic bile ducts. A therapeutic approach. A case of congenital hepatic fibrosis with dilation of the intrahepatic ducts is presented. External drainage and lavage of the biliary tract through a prosthetic \"Y\" tube and internal drainage accomplished by a Roux en-Y cholangiojejunostomy resulted in prevention of the patient's recurrent bouts of cholangitis and sepsis. Surgical therapy for recurrent cholangitis in this disease is reviewed.", "PMID": 964576}
{"id": "pubmed23n0032_5994", "title": "Acute suppurative cholangitis secondary to calcific pancreatitis.", "content": "In the absence of calculi or carcinoma, the occurrence of acute suppurative cholangitis secondary to calcific pancreatitis is extremely rare. This report describes the successful management of acute suppurative cholangitis by surgical decompression and use of a new aminoglycoside, amikacin.", "contents": "Acute suppurative cholangitis secondary to calcific pancreatitis. In the absence of calculi or carcinoma, the occurrence of acute suppurative cholangitis secondary to calcific pancreatitis is extremely rare. This report describes the successful management of acute suppurative cholangitis by surgical decompression and use of a new aminoglycoside, amikacin.", "PMID": 964577}
{"id": "pubmed23n0032_5995", "title": "Dimensions of adolescent psychopathology and their prognostic significance for treatment outcome.", "content": "The mothers of 87 male and female adolescents accepted at a counseling agency described their offspring by completing the Institute of Juvenile Research Behavior Checklist. A factor analysis of the initial data yielded nine symptom factors. These were used in combination with selected demographic variables, to compare the 54 continuers in treatment with the 22 early defectors and the 18 cases improved after a year of a full course of treatment with the 16 unimproved cases. Both discriminant function analyses indicated that the groups could be successfully differentiated; however, variables most predictive of continuance in treatment were not most predictive of a successful outcome.", "contents": "Dimensions of adolescent psychopathology and their prognostic significance for treatment outcome. The mothers of 87 male and female adolescents accepted at a counseling agency described their offspring by completing the Institute of Juvenile Research Behavior Checklist. A factor analysis of the initial data yielded nine symptom factors. These were used in combination with selected demographic variables, to compare the 54 continuers in treatment with the 22 early defectors and the 18 cases improved after a year of a full course of treatment with the 16 unimproved cases. Both discriminant function analyses indicated that the groups could be successfully differentiated; however, variables most predictive of continuance in treatment were not most predictive of a successful outcome.", "PMID": 964593}
{"id": "pubmed23n0032_5996", "title": "Accelerated conservation acquisition and IQ gains by blind children.", "content": "Because blind chidlren are retarded in the attainment of substance conservation and other Piagetial concepts, it was hypothesized that a learning set training procedure which incorporated both substance conservation and subordinate concepts might accelerate the acquisition of substance conservation itself and promote generalization to weight conservation scores and IQ. To test the hypotheses, 28 blind children aged 8 to 13 (IQ range 62 to 131) were divided into two groups. Their visual capacities ranged from 20/500 in the best eye to total congenital blindness and etiologies were varied, but consisted primarily of cataracts and glaucoma. All children were pretested and posttested on Slosson's Intelligence Test, 40 substance conservation tasks, and 20 weight conservation tasks, which involved both continuous and discontinuous substance and included almost all those found in the literature prior to 1970. A lengthy learning set procedure employing everyday household objects was then used to teach the experimental children conceptual skills that are prerequisites to substance conservation. Finally the learning set procedure was used to teach the concept of substance conservation itself to the children. The trained group exhibited significantly increased substance and weight conservation scores and IQs (all ps less than .005). The control group, which had spent matched periods of time in intellectual enrichment activities failed to improve significantly on any of the dependent variables. The research suggests that acceleration of the development of Piagetian concepts may be profitable enterprise in the case of blind children.", "contents": "Accelerated conservation acquisition and IQ gains by blind children. Because blind chidlren are retarded in the attainment of substance conservation and other Piagetial concepts, it was hypothesized that a learning set training procedure which incorporated both substance conservation and subordinate concepts might accelerate the acquisition of substance conservation itself and promote generalization to weight conservation scores and IQ. To test the hypotheses, 28 blind children aged 8 to 13 (IQ range 62 to 131) were divided into two groups. Their visual capacities ranged from 20/500 in the best eye to total congenital blindness and etiologies were varied, but consisted primarily of cataracts and glaucoma. All children were pretested and posttested on Slosson's Intelligence Test, 40 substance conservation tasks, and 20 weight conservation tasks, which involved both continuous and discontinuous substance and included almost all those found in the literature prior to 1970. A lengthy learning set procedure employing everyday household objects was then used to teach the experimental children conceptual skills that are prerequisites to substance conservation. Finally the learning set procedure was used to teach the concept of substance conservation itself to the children. The trained group exhibited significantly increased substance and weight conservation scores and IQs (all ps less than .005). The control group, which had spent matched periods of time in intellectual enrichment activities failed to improve significantly on any of the dependent variables. The research suggests that acceleration of the development of Piagetian concepts may be profitable enterprise in the case of blind children.", "PMID": 964594}
{"id": "pubmed23n0032_5997", "title": "Factor VII levels as a guide to prognosis in fulminant hepatic failure.", "content": "Levels of clotting factors II, V, and VII were measured on admission and then daily in 12 patients with grade IV hepatic coma due to fulminant hepatic failure. Factor VII levels obtained within 36 hours of the development of grade IV coma were not of value in predicting which patients would subsequently recover consciousness. Four of the latter group had levels below 9% at this time while the levels in three of the seven fatal cases were higher. Serial determinations were of more value and levels rose rapidly in those patients who ultimately made a complete recovery.", "contents": "Factor VII levels as a guide to prognosis in fulminant hepatic failure. Levels of clotting factors II, V, and VII were measured on admission and then daily in 12 patients with grade IV hepatic coma due to fulminant hepatic failure. Factor VII levels obtained within 36 hours of the development of grade IV coma were not of value in predicting which patients would subsequently recover consciousness. Four of the latter group had levels below 9% at this time while the levels in three of the seven fatal cases were higher. Serial determinations were of more value and levels rose rapidly in those patients who ultimately made a complete recovery.", "PMID": 964680}
{"id": "pubmed23n0032_5998", "title": "Focal nodular hyperplasia of the liver: a rational approach to treatment.", "content": "Focal nodular hyperplasia of the liver in a 3 year old child has been successfully treated by ligation of the hepatic artery branches supplying the lesion. The prominence of the abnormal vasculature in the lesion and its possible aetiological involvement is stressed. Because of the risks of partial hepatectomy, hepatic artery ligation is suggested as the treatment of choice for focal nodular hyperplasia.", "contents": "Focal nodular hyperplasia of the liver: a rational approach to treatment. Focal nodular hyperplasia of the liver in a 3 year old child has been successfully treated by ligation of the hepatic artery branches supplying the lesion. The prominence of the abnormal vasculature in the lesion and its possible aetiological involvement is stressed. Because of the risks of partial hepatectomy, hepatic artery ligation is suggested as the treatment of choice for focal nodular hyperplasia.", "PMID": 964681}
{"id": "pubmed23n0032_5999", "title": "Abnormalities of sodium excretion and other disorders of renal function in fulminant hepatic failure.", "content": "Renal function was evaluated in 40 patients with fulminant hepatic failure, They were divided into two groups on the basis of glomerular filtration rates greater than 40 ml/min or less than 25 ml/min. A number of patients in group 1 had markedly abnormal renal retention of sodium together with a reduced free water clearance and low potassium excretion which could be explained by increased proximal tubular reabsorption of sodium. The patients in group 2 had evidence that renal tubular integrity was maintained when the glomerular filtration rate was greater than or equal ml/min (functional renal failure), but evidence of tubular damage was present when this was less than 3 ml/min (acute tubular necrosis).", "contents": "Abnormalities of sodium excretion and other disorders of renal function in fulminant hepatic failure. Renal function was evaluated in 40 patients with fulminant hepatic failure, They were divided into two groups on the basis of glomerular filtration rates greater than 40 ml/min or less than 25 ml/min. A number of patients in group 1 had markedly abnormal renal retention of sodium together with a reduced free water clearance and low potassium excretion which could be explained by increased proximal tubular reabsorption of sodium. The patients in group 2 had evidence that renal tubular integrity was maintained when the glomerular filtration rate was greater than or equal ml/min (functional renal failure), but evidence of tubular damage was present when this was less than 3 ml/min (acute tubular necrosis).", "PMID": 964682}
{"id": "pubmed23n0032_6000", "title": "Intestinal perfusion studies in tropical sprue. 1. Amino acid and dipeptide absorption.", "content": "Intestinal absorption of glycine 20 mmol/1, glycyl-glycine 10 mmol/1 plus L-leucine 10 mmol/1, and glycyl-L-leucine 10 mmol/1 has been studied by intestinal perfusion in 11 patients with tropical sprue and 10 control subjects. The patients with sprue had a significant reduction in the rate of absorption of glycine from a 20 mmol/1 solution, but there were no significant differences in the absorption of the other substances. The failure to demonstrate any difference in the absorption of these substances is probably related to their low concentration relative to the maximum absorptive capacity of the intestine. In both groups of subjects the kinetic advantage of glycyl-glycine absorption as compared with glycine absorption was maintained. When the dipeptides were perfused, free amino acids appeared in the perfusate presumably by \"back diffusion\" from the mucosal cells. In the case of glycyl-L-leucine considerably more glycine and leucine were found in the perfusate in patients with sprue than in the control subjects. There was no correlation between peptide absorption and the concentration of total glycly-glycine hydrolase and glycyl-L-leucine hydrolase, measured as combined brush border and cytosol enzymes. The concentrations of these enzymes were similar in both groups of subjects.", "contents": "Intestinal perfusion studies in tropical sprue. 1. Amino acid and dipeptide absorption. Intestinal absorption of glycine 20 mmol/1, glycyl-glycine 10 mmol/1 plus L-leucine 10 mmol/1, and glycyl-L-leucine 10 mmol/1 has been studied by intestinal perfusion in 11 patients with tropical sprue and 10 control subjects. The patients with sprue had a significant reduction in the rate of absorption of glycine from a 20 mmol/1 solution, but there were no significant differences in the absorption of the other substances. The failure to demonstrate any difference in the absorption of these substances is probably related to their low concentration relative to the maximum absorptive capacity of the intestine. In both groups of subjects the kinetic advantage of glycyl-glycine absorption as compared with glycine absorption was maintained. When the dipeptides were perfused, free amino acids appeared in the perfusate presumably by \"back diffusion\" from the mucosal cells. In the case of glycyl-L-leucine considerably more glycine and leucine were found in the perfusate in patients with sprue than in the control subjects. There was no correlation between peptide absorption and the concentration of total glycly-glycine hydrolase and glycyl-L-leucine hydrolase, measured as combined brush border and cytosol enzymes. The concentrations of these enzymes were similar in both groups of subjects.", "PMID": 964683}
{"id": "pubmed23n0032_6001", "title": "Permeability of the small intestine after intra-arterial injection of histamine-type mediators and irradiation.", "content": "Permeability and selectivity of rabbit small intestine were estimated by a perfusion technique after intra-arterial injection of histamine-type mediators and intestinal irradiation. It was shown that the histamine-type mediators caused an increase in capillary permeability which produced an overall moderate increase in transmucosal permeability with a moderate loss of selectivity. Local intestinal irradiation caused a very marked increase in permeability and a profound loss of selectivity. It was felt that this was produced partly by an increase in capillary permeability but largely by damage to the epithelial basement membrane. It is concluded that the intestinal capillary endothelium is both rate-limiting and selective, though not to a major degree in either case. The epithelial basement membrane, however, appears to be both rate-limiting and markedly selective.", "contents": "Permeability of the small intestine after intra-arterial injection of histamine-type mediators and irradiation. Permeability and selectivity of rabbit small intestine were estimated by a perfusion technique after intra-arterial injection of histamine-type mediators and intestinal irradiation. It was shown that the histamine-type mediators caused an increase in capillary permeability which produced an overall moderate increase in transmucosal permeability with a moderate loss of selectivity. Local intestinal irradiation caused a very marked increase in permeability and a profound loss of selectivity. It was felt that this was produced partly by an increase in capillary permeability but largely by damage to the epithelial basement membrane. It is concluded that the intestinal capillary endothelium is both rate-limiting and selective, though not to a major degree in either case. The epithelial basement membrane, however, appears to be both rate-limiting and markedly selective.", "PMID": 964684}
{"id": "pubmed23n0032_6002", "title": "Influence of staphylococcal enterotoxin on water and electrolyte transport in the small intestine.", "content": "The direct and indirect effects of staphylococcal enterotoxins A and C on the flux of water, sodium, and potassium have been studied in paired Thiry Vella fistulae in dogs. Administration of toxin resulted in a significant decrease in absorption, both in the loop to which the toxin had been administered and its pair. This decrease in absorption was associated with a decrease in movement out of the lumen, movement into the lumen remaining relatively unchanged. The mechanism of action of staphylococcal enterotoxins is discussed, and comparisons made with other enterotoxins.", "contents": "Influence of staphylococcal enterotoxin on water and electrolyte transport in the small intestine. The direct and indirect effects of staphylococcal enterotoxins A and C on the flux of water, sodium, and potassium have been studied in paired Thiry Vella fistulae in dogs. Administration of toxin resulted in a significant decrease in absorption, both in the loop to which the toxin had been administered and its pair. This decrease in absorption was associated with a decrease in movement out of the lumen, movement into the lumen remaining relatively unchanged. The mechanism of action of staphylococcal enterotoxins is discussed, and comparisons made with other enterotoxins.", "PMID": 964685}
{"id": "pubmed23n0032_6003", "title": "Effect of gastrin on gastric mucosal cell proliferation in man.", "content": "The effect of short-term infusion of a large dose of pentagastrin and a small dose of synthetic human gastrin I (SHG) on the rate of cell proliferation in gastric mucosa was studied in normal human subjects. Moreover, the kinetic parameters were compared with the serum gastrin concentrations in fasting patients. Endoscopic biopsies were labelled in vitro with 3H-thymidine and autoradiographs were prepared. The percentage of DNA-synthesising cells in the progenitor cell region was estimated. In healthy volunteers infusion of a large dose of pentagastrin (10 mug/kg per hour) was followed by a marked increase in the labelling index in fundic mucosa. The antral mucosa was not responsive to this effect. In the same subjects, infusion of a low dose of SHG (8 ng/kg per min) did not affect the rate of cell proliferation, either in fundic or in antral mucosa. In 46 patients with different gastric diseases no correlation between the serum gastrin concentrations and the labelling indices was found. The results suggest that human fundic mucosa is responsive to a trophic action of pentagastrin. If it exists, however, a physiological action of gastrin as a trophic hormone for human gastric mucosa must be considerably more complex than previously believed.", "contents": "Effect of gastrin on gastric mucosal cell proliferation in man. The effect of short-term infusion of a large dose of pentagastrin and a small dose of synthetic human gastrin I (SHG) on the rate of cell proliferation in gastric mucosa was studied in normal human subjects. Moreover, the kinetic parameters were compared with the serum gastrin concentrations in fasting patients. Endoscopic biopsies were labelled in vitro with 3H-thymidine and autoradiographs were prepared. The percentage of DNA-synthesising cells in the progenitor cell region was estimated. In healthy volunteers infusion of a large dose of pentagastrin (10 mug/kg per hour) was followed by a marked increase in the labelling index in fundic mucosa. The antral mucosa was not responsive to this effect. In the same subjects, infusion of a low dose of SHG (8 ng/kg per min) did not affect the rate of cell proliferation, either in fundic or in antral mucosa. In 46 patients with different gastric diseases no correlation between the serum gastrin concentrations and the labelling indices was found. The results suggest that human fundic mucosa is responsive to a trophic action of pentagastrin. If it exists, however, a physiological action of gastrin as a trophic hormone for human gastric mucosa must be considerably more complex than previously believed.", "PMID": 964686}
{"id": "pubmed23n0032_6004", "title": "Gastric emptying in control subjects and patients with duodenal ulcer before and after vagotomy.", "content": "The emptying of a solid meal labelled with Indium 113mDTPA from the stomach was studied with a gamma camera in 26 normal subjects, 27 patients with duodenal ulcer, on 41 occasions after truncal vagotomy and pyloroplasty and 38 times after highly selective vagotomy. Applying the method of principal component analysis to the results, differences were detected between control and duodenal ulcer subjects and two probable subgroups of duodenal ulcer were observed. Half emptying times did not reveal these patterns. After vagotomy, delayed emptying was general at one week. At one month, patients after highly selective vagotomy had a more normal result than those with truncal vagotomy and pyloroplasty (TV), but by six months no significant difference in overall emptying rate was found, although changes in the pattern of gastric emptying persisted in some patients after TV.", "contents": "Gastric emptying in control subjects and patients with duodenal ulcer before and after vagotomy. The emptying of a solid meal labelled with Indium 113mDTPA from the stomach was studied with a gamma camera in 26 normal subjects, 27 patients with duodenal ulcer, on 41 occasions after truncal vagotomy and pyloroplasty and 38 times after highly selective vagotomy. Applying the method of principal component analysis to the results, differences were detected between control and duodenal ulcer subjects and two probable subgroups of duodenal ulcer were observed. Half emptying times did not reveal these patterns. After vagotomy, delayed emptying was general at one week. At one month, patients after highly selective vagotomy had a more normal result than those with truncal vagotomy and pyloroplasty (TV), but by six months no significant difference in overall emptying rate was found, although changes in the pattern of gastric emptying persisted in some patients after TV.", "PMID": 964687}
{"id": "pubmed23n0032_6005", "title": "Gastric morphology and serum gastrin levels in pernicious anaemia.", "content": "Mucosal biopsies from multiple sites in the stomachs of 21 patients with pernicious anaemia have been examined. The histological changes almost always involved the entire gastric mucosa, including that of the pyloric antrum. Metaplastic changes were almost universal and consisted of intestinal metaplasia in the body and antrum and pyloric metaplasia in the body. The severity of the pyloric metaplasia was such as to make the distinction between body and antrum on biopsy impossible. No relationship was found between serum gastrin activity and the histological appearances of the gastric antrum or body.", "contents": "Gastric morphology and serum gastrin levels in pernicious anaemia. Mucosal biopsies from multiple sites in the stomachs of 21 patients with pernicious anaemia have been examined. The histological changes almost always involved the entire gastric mucosa, including that of the pyloric antrum. Metaplastic changes were almost universal and consisted of intestinal metaplasia in the body and antrum and pyloric metaplasia in the body. The severity of the pyloric metaplasia was such as to make the distinction between body and antrum on biopsy impossible. No relationship was found between serum gastrin activity and the histological appearances of the gastric antrum or body.", "PMID": 964688}
{"id": "pubmed23n0032_6006", "title": "Tissue demonstration of carcinoembryonic antigen (CEA) in ulcerative colitis.", "content": "An immunoperoxidase technique was used to demonstrate carcinoembryonic antigen (CEA) in sections of proctocolectomy specimens from nine cases of ulcerative colitis in some of which carcinoma and premalignant change had developed. Carcinoma and premalignant change stained positively for CEA in contrast with benign mucosa, whether or not inflammatory dysplasia was present.", "contents": "Tissue demonstration of carcinoembryonic antigen (CEA) in ulcerative colitis. An immunoperoxidase technique was used to demonstrate carcinoembryonic antigen (CEA) in sections of proctocolectomy specimens from nine cases of ulcerative colitis in some of which carcinoma and premalignant change had developed. Carcinoma and premalignant change stained positively for CEA in contrast with benign mucosa, whether or not inflammatory dysplasia was present.", "PMID": 964689}
{"id": "pubmed23n0032_6007", "title": "Cell contacts and distribution of nerves in the smooth muscle of estrogen-dominated rabbit oviduct.", "content": "The types of cell-to-cell contacts and the relationship between the nerve endings and the smooth muscle of the estrogen-treated rabbit oviduct have been investigated. The cell contacts most consistently found in the oviduct were simple appositions, with a 20-nm cell separation, which occasionally included a more dense region, and interdigitations of muscle cell processes with the same separation distances. Very rarely, wider intermediate contacts were present. Gap junctions (nexuses) were absent. It seems likely that simple appositions and interdigitations form the low resistance pathway for conduction of electrical activity. Nerves containing small dense-cored vesicles were present both within and near muscle bundles; they were rarely found close to smooth muscle cells.", "contents": "Cell contacts and distribution of nerves in the smooth muscle of estrogen-dominated rabbit oviduct. The types of cell-to-cell contacts and the relationship between the nerve endings and the smooth muscle of the estrogen-treated rabbit oviduct have been investigated. The cell contacts most consistently found in the oviduct were simple appositions, with a 20-nm cell separation, which occasionally included a more dense region, and interdigitations of muscle cell processes with the same separation distances. Very rarely, wider intermediate contacts were present. Gap junctions (nexuses) were absent. It seems likely that simple appositions and interdigitations form the low resistance pathway for conduction of electrical activity. Nerves containing small dense-cored vesicles were present both within and near muscle bundles; they were rarely found close to smooth muscle cells.", "PMID": 964720}
{"id": "pubmed23n0032_6008", "title": "Bulk flows through human fetal membranes.", "content": "Bulk water flows across term human amnio-chorion are studied in vitro. The hydrodynamic permeability is found to vary with both hydrostatic and osmotic pressure. The coefficient characterizing flows generated by hydrostatic pressure is substantially larger than that characterizing osmotic flows. The measurements are utilised to predict in vivo bulk flows across the amnio-chorion. These lead to the prediction that at most a flux of 34-83 ml/day may occur across amnio-chorion directed outwards from the amniotic cavity, the principal contribution to this arising from the effects of hydrostatic pressure.", "contents": "Bulk flows through human fetal membranes. Bulk water flows across term human amnio-chorion are studied in vitro. The hydrodynamic permeability is found to vary with both hydrostatic and osmotic pressure. The coefficient characterizing flows generated by hydrostatic pressure is substantially larger than that characterizing osmotic flows. The measurements are utilised to predict in vivo bulk flows across the amnio-chorion. These lead to the prediction that at most a flux of 34-83 ml/day may occur across amnio-chorion directed outwards from the amniotic cavity, the principal contribution to this arising from the effects of hydrostatic pressure.", "PMID": 964721}
{"id": "pubmed23n0032_6009", "title": "Permeability of chorion laeve to succinylcholine in vitro.", "content": "The simultaneous diffusion of 14C-succinylcholine and p-amino hippurate across human placental tissue in vitro was measured. Column chromatographic purification of the radioactivity followed by paper chromatographic analysis showed little alteration of the succinylcholine during the course of these experiments. Similar chloroform-buffer partition coefficients for the two solutes were measured. Thus in these experiments, the nearly identical transfer rates of these two similar sized, lipid-insoluble compounds indicated that succinylcholine crosses human placental tissue in quantitites compatible with its molecular size and lipophobic nature, and that the diffusion rate is not significantly influenced by its increased molecular charge.", "contents": "Permeability of chorion laeve to succinylcholine in vitro. The simultaneous diffusion of 14C-succinylcholine and p-amino hippurate across human placental tissue in vitro was measured. Column chromatographic purification of the radioactivity followed by paper chromatographic analysis showed little alteration of the succinylcholine during the course of these experiments. Similar chloroform-buffer partition coefficients for the two solutes were measured. Thus in these experiments, the nearly identical transfer rates of these two similar sized, lipid-insoluble compounds indicated that succinylcholine crosses human placental tissue in quantitites compatible with its molecular size and lipophobic nature, and that the diffusion rate is not significantly influenced by its increased molecular charge.", "PMID": 964722}
{"id": "pubmed23n0032_6010", "title": "Suicides committed by minors.", "content": "The bodies of 76 minors, below 18 years of age, who died as a consequence of committing suicide during the years 1960 to 1974 were examined in the Institute of Forensic Medicine in Cracow. The youngest victim was a boy 8 1/2 years of age. The majority of cases (63) referred to minors aged 16-18. The deaths were due to: hanging (41 cases), poisoning (17 cases), leaping from a height (7 cases), and others (11 cases including one case of self shooting). Among the motives for suicide the first place is taken by personal troubles, mostly school failures, then bad health and family conflicts. Prior suicidal attempts were noted in 13 cases and in 10 others oral declarations of suicide were made. Motives for suicide essentially differ from those found in earlier studies from the years 1881-1939, where ill luck in love and undesired pregnancy were treated as one of the important factors leading to suicide.", "contents": "Suicides committed by minors. The bodies of 76 minors, below 18 years of age, who died as a consequence of committing suicide during the years 1960 to 1974 were examined in the Institute of Forensic Medicine in Cracow. The youngest victim was a boy 8 1/2 years of age. The majority of cases (63) referred to minors aged 16-18. The deaths were due to: hanging (41 cases), poisoning (17 cases), leaping from a height (7 cases), and others (11 cases including one case of self shooting). Among the motives for suicide the first place is taken by personal troubles, mostly school failures, then bad health and family conflicts. Prior suicidal attempts were noted in 13 cases and in 10 others oral declarations of suicide were made. Motives for suicide essentially differ from those found in earlier studies from the years 1881-1939, where ill luck in love and undesired pregnancy were treated as one of the important factors leading to suicide.", "PMID": 964801}
{"id": "pubmed23n0032_6011", "title": "The quantitative analysis of glass by emission spectrography: a six element survey.", "content": "The use of emission spectrography for the analysis of small glass fragments (approximately 1 mg) is described. Levels of Al, Ba, Ca, Fe, Mg and Mn have been determined in approximately 200 samples, and with the exception of Ca their variation appears to be of value in the classification and/or discrimination of glass. The precision of the method has been studied and its accuracy determined by the analysis of standard glasses prepared in the laboratory.", "contents": "The quantitative analysis of glass by emission spectrography: a six element survey. The use of emission spectrography for the analysis of small glass fragments (approximately 1 mg) is described. Levels of Al, Ba, Ca, Fe, Mg and Mn have been determined in approximately 200 samples, and with the exception of Ca their variation appears to be of value in the classification and/or discrimination of glass. The precision of the method has been studied and its accuracy determined by the analysis of standard glasses prepared in the laboratory.", "PMID": 964804}
{"id": "pubmed23n0032_6012", "title": "Homicide in acute porphyria.", "content": "The subject was a young man known to suffer from the intermittent (Swedish) form of this disorder, who committed homicide in an acute attack of porphyria and who died from the disease 7 days later. The family history and clinical features of the acute attack are reported. The paper includes a brief review of the literature, with reference to the psychiatric features of acute porphyria.", "contents": "Homicide in acute porphyria. The subject was a young man known to suffer from the intermittent (Swedish) form of this disorder, who committed homicide in an acute attack of porphyria and who died from the disease 7 days later. The family history and clinical features of the acute attack are reported. The paper includes a brief review of the literature, with reference to the psychiatric features of acute porphyria.", "PMID": 964803}
{"id": "pubmed23n0032_6013", "title": "[Epithelial barriers of the intestine. Significance and function in defense against infection].", "content": "The epithelial surface of the intestinal tract provides for an important barrier between the organism and its environment. However, it permits the passage into the tissues of limited amounts of macromolecules and/or particles, especially bacteria. These functions are age- and species-dependent, and they are closely related to nonspecific and specific immune reactions. Continuous and intimate contact between antigenic material from the gut lumen and immunocompetent cells takes place in gut-associated lymphoid tissues.", "contents": "[Epithelial barriers of the intestine. Significance and function in defense against infection]. The epithelial surface of the intestinal tract provides for an important barrier between the organism and its environment. However, it permits the passage into the tissues of limited amounts of macromolecules and/or particles, especially bacteria. These functions are age- and species-dependent, and they are closely related to nonspecific and specific immune reactions. Continuous and intimate contact between antigenic material from the gut lumen and immunocompetent cells takes place in gut-associated lymphoid tissues.", "PMID": 964810}
{"id": "pubmed23n0032_6014", "title": "[Mushroom poisoning. New possibilities for treatment].", "content": "Poisonous species of fungi in Germany are very few. Dangerous is the ingestion of raw, spoiled or poisonous mushrooms. There exist no reliable tests to determine whether a mushroom is safe except by expert examination and identification of the mushroom. In clinical practice the classification of mushroom poisoning is possible in muscarine-syndrome, gastroenteritic syndrome and in two-phase-syndrome. 90-95% of lethal mushroom poisonings are due to ingestion of Amanita phalloides. In severe cases extensive hepatic necrosis occurs, characterized by profound abnormalities in liver function caused by hepatic coma. In deep coma mortality rates amount to 70% or more. A new therapeutic measure (coated charcoal hemoperfusion)-first applied in liver failure by Chang (1972) and Williams (1973)-has been performed in 3 patients with severe poisoning after ingestion of Amanita phalloides (each patient had eaten at least 7-10 fungi Amanita phalloides). Two of the patients survived.", "contents": "[Mushroom poisoning. New possibilities for treatment]. Poisonous species of fungi in Germany are very few. Dangerous is the ingestion of raw, spoiled or poisonous mushrooms. There exist no reliable tests to determine whether a mushroom is safe except by expert examination and identification of the mushroom. In clinical practice the classification of mushroom poisoning is possible in muscarine-syndrome, gastroenteritic syndrome and in two-phase-syndrome. 90-95% of lethal mushroom poisonings are due to ingestion of Amanita phalloides. In severe cases extensive hepatic necrosis occurs, characterized by profound abnormalities in liver function caused by hepatic coma. In deep coma mortality rates amount to 70% or more. A new therapeutic measure (coated charcoal hemoperfusion)-first applied in liver failure by Chang (1972) and Williams (1973)-has been performed in 3 patients with severe poisoning after ingestion of Amanita phalloides (each patient had eaten at least 7-10 fungi Amanita phalloides). Two of the patients survived.", "PMID": 964811}
{"id": "pubmed23n0032_6015", "title": "Evaluation of column extraction: a new procedure for the analysis of drugs in body fluids.", "content": "A method is described for the extraction of drugs from body fluids, whereby liquid-liquid extraction is replaced by liquid-solid elution. The aqueous sample is absorbed on a column filled with dry supporting material. By elution with organic solvents lipophilic substances are extracted from the water phase which remains on the column. The eluate containing the drugs is free of emulsions. For optimal extraction any pH value and a variety of solvents can be used. Column extraction of urine, serum and blood is easily performed and gives high recovery rates, which are superior to conventional extraction. Thin layer chromatography of drugs following conventional XAD-2 and column extraction, demonstrates identical qualitative results, but shows higher purity of the column extracts. The sensitivity of detection is enhanced.", "contents": "Evaluation of column extraction: a new procedure for the analysis of drugs in body fluids. A method is described for the extraction of drugs from body fluids, whereby liquid-liquid extraction is replaced by liquid-solid elution. The aqueous sample is absorbed on a column filled with dry supporting material. By elution with organic solvents lipophilic substances are extracted from the water phase which remains on the column. The eluate containing the drugs is free of emulsions. For optimal extraction any pH value and a variety of solvents can be used. Column extraction of urine, serum and blood is easily performed and gives high recovery rates, which are superior to conventional extraction. Thin layer chromatography of drugs following conventional XAD-2 and column extraction, demonstrates identical qualitative results, but shows higher purity of the column extracts. The sensitivity of detection is enhanced.", "PMID": 964805}
{"id": "pubmed23n0032_6016", "title": "[Antibiotic therapy for infectious enteritis in childhood].", "content": "A brief review of the actual knowledge on the infantile enteritis caused by infectious agents is given. The antibiotic therapy of bacterial enteritis is still acute, but the range of indications is limited. This kind of therapy aims at the curative effect, but not at the sanitation of the stools, for which no reliable method exists. Any kind of infantile enteritis needs dietetic treatment.", "contents": "[Antibiotic therapy for infectious enteritis in childhood]. A brief review of the actual knowledge on the infantile enteritis caused by infectious agents is given. The antibiotic therapy of bacterial enteritis is still acute, but the range of indications is limited. This kind of therapy aims at the curative effect, but not at the sanitation of the stools, for which no reliable method exists. Any kind of infantile enteritis needs dietetic treatment.", "PMID": 964812}
{"id": "pubmed23n0032_6017", "title": "The increasing use of paraquat as a suicidal agent.", "content": "Paraquat, the most important of the bipyridyl group of herbicides, has proved a most effective weedkiller and is now in world-wide use. When used as intended, the compound is quite safe, but misuse has led to a significant number of deaths. If taken orally in significant amounts a fatal outcome is likely and there is as yet no effective antidote. Deaths from paraquat poisoning have been proportionately higher in Ireland than elsewhere. The present paper analyses 26 fatal and 7 non-fatal poisonings occurring in Northern Ireland during the period 1967-1975. The history, clinical features, length of survival and pathological findings are discussed. Extensive toxicological analyses have been performed on samples taken during life, and on a wide range of body fluids and tissues obtained at autopsy. These data are presented and show the progressive but slow fall-off in paraquat recovery after ingestion, with detectable levels still present for up to 21 days. The increasing incidence of suicidal, as opposed to accidental, paraquat poisoning is stressed. The decline in accidental poisoning suggests that the measures taken to prevent accidental ingestion are proving successful. The swing from accident to suicide is reflected in the shorter survival times, differing autopsy findings and significantly higher tissue and body fluid levels encountered in suicidal poisonings.", "contents": "The increasing use of paraquat as a suicidal agent. Paraquat, the most important of the bipyridyl group of herbicides, has proved a most effective weedkiller and is now in world-wide use. When used as intended, the compound is quite safe, but misuse has led to a significant number of deaths. If taken orally in significant amounts a fatal outcome is likely and there is as yet no effective antidote. Deaths from paraquat poisoning have been proportionately higher in Ireland than elsewhere. The present paper analyses 26 fatal and 7 non-fatal poisonings occurring in Northern Ireland during the period 1967-1975. The history, clinical features, length of survival and pathological findings are discussed. Extensive toxicological analyses have been performed on samples taken during life, and on a wide range of body fluids and tissues obtained at autopsy. These data are presented and show the progressive but slow fall-off in paraquat recovery after ingestion, with detectable levels still present for up to 21 days. The increasing incidence of suicidal, as opposed to accidental, paraquat poisoning is stressed. The decline in accidental poisoning suggests that the measures taken to prevent accidental ingestion are proving successful. The swing from accident to suicide is reflected in the shorter survival times, differing autopsy findings and significantly higher tissue and body fluid levels encountered in suicidal poisonings.", "PMID": 964807}
{"id": "pubmed23n0032_6018", "title": "Aspects of the hyoid-larynx complex in forensic pathology.", "content": "Post-mortem radiological studies of the hyoid-larynx complex on excised specimens were undertaken. The post-mortem specimens were fixed to polysterene boards by ratio-translucent plastic pins. Radiological examinations revealed a fracture of the left greater cornu of the hyoid bone in a case of suicidal hanging; a fracture through the calcified right superior cornu of the thyroid cartilage in a case of blunt trauma to the left side of the head and neck; and a fracture artefact through the middle of the left greater cornu of the hyoid bone through compression of the structure by manipulation during the process of removal of the neck structures. In each instance the fracture sites were exposed and photographed. The use of a technique which permits a radiological examination of free dissected specimens of the hyoid-larynx complex has many advantages in the medicolegal investigations of injuries of the neck.", "contents": "Aspects of the hyoid-larynx complex in forensic pathology. Post-mortem radiological studies of the hyoid-larynx complex on excised specimens were undertaken. The post-mortem specimens were fixed to polysterene boards by ratio-translucent plastic pins. Radiological examinations revealed a fracture of the left greater cornu of the hyoid bone in a case of suicidal hanging; a fracture through the calcified right superior cornu of the thyroid cartilage in a case of blunt trauma to the left side of the head and neck; and a fracture artefact through the middle of the left greater cornu of the hyoid bone through compression of the structure by manipulation during the process of removal of the neck structures. In each instance the fracture sites were exposed and photographed. The use of a technique which permits a radiological examination of free dissected specimens of the hyoid-larynx complex has many advantages in the medicolegal investigations of injuries of the neck.", "PMID": 964808}
{"id": "pubmed23n0032_6019", "title": "[Acupuncture, Theory and practice].", "content": "Trasditional Chinese medicine is based on inductive-synthetic reasoning with regard to all life processes, in contrast to the causal-analytic orientation in our Western medicine. This philosophical approach is based on a system of comparisons (equivalents), referred to as Yin and Yang. Vital functions are seen as \"energizing\" processes, whereby the acupuncture points and the system of meridians are seen as a means of regulation for this \"energy\". A disturbed balance in this energy results in illness. The therapy according to the acupuncture method is to acheive a balance in the disturbed flow of energy by means of acupuncture points. By utilizing biophysical methods of measurement, it is possible to prove that a change in this energy takes place. The complex active principle which results in the effectiveness of acupuncture includes neurophysiological, biochemical and biophysical functional processes.", "contents": "[Acupuncture, Theory and practice]. Trasditional Chinese medicine is based on inductive-synthetic reasoning with regard to all life processes, in contrast to the causal-analytic orientation in our Western medicine. This philosophical approach is based on a system of comparisons (equivalents), referred to as Yin and Yang. Vital functions are seen as \"energizing\" processes, whereby the acupuncture points and the system of meridians are seen as a means of regulation for this \"energy\". A disturbed balance in this energy results in illness. The therapy according to the acupuncture method is to acheive a balance in the disturbed flow of energy by means of acupuncture points. By utilizing biophysical methods of measurement, it is possible to prove that a change in this energy takes place. The complex active principle which results in the effectiveness of acupuncture includes neurophysiological, biochemical and biophysical functional processes.", "PMID": 964816}
{"id": "pubmed23n0032_6020", "title": "[The thermal trigeminal impulse test. A method for more precise trigeminal nerve testing].", "content": "A new method is described, which determines the subjective sensitivity-level for warmth-impulses in the innervation-areas of trigeminus in the face. This method seems to be more sensitive than methods for judging the tactile sensitivity. The warmth-sensitivity shows no correlation to age and sex; the lowest level was found in the innervation area of the 2nd branch of trigeminus.", "contents": "[The thermal trigeminal impulse test. A method for more precise trigeminal nerve testing]. A new method is described, which determines the subjective sensitivity-level for warmth-impulses in the innervation-areas of trigeminus in the face. This method seems to be more sensitive than methods for judging the tactile sensitivity. The warmth-sensitivity shows no correlation to age and sex; the lowest level was found in the innervation area of the 2nd branch of trigeminus.", "PMID": 964817}
{"id": "pubmed23n0032_6021", "title": "[Phlebography of the legs].", "content": "On the basis of more than 10,000 phlebographies we believe that the ascending phlebography with control by means of x-rays and accurate photographs, the so-called \"phleboscopy\", is the most appropriate of all techniques of phlebography for a routine ambulant examination of the veins. By means of phlebography we succeed in employing our therapeutical measures accurately, control of therapeutic results is possible. The phlebography informs about the morphological condition of the veinous system and the defluxion conditions. It serves for determining pathological conditions, such as the post-thrombotical syndrom in the case of acute deep thrombosis of the crural veins, and informs about the extent and defluxion conditions of the varicose veins as well as the sufficiency or insufficiency of the Vv. perforantes.", "contents": "[Phlebography of the legs]. On the basis of more than 10,000 phlebographies we believe that the ascending phlebography with control by means of x-rays and accurate photographs, the so-called \"phleboscopy\", is the most appropriate of all techniques of phlebography for a routine ambulant examination of the veins. By means of phlebography we succeed in employing our therapeutical measures accurately, control of therapeutic results is possible. The phlebography informs about the morphological condition of the veinous system and the defluxion conditions. It serves for determining pathological conditions, such as the post-thrombotical syndrom in the case of acute deep thrombosis of the crural veins, and informs about the extent and defluxion conditions of the varicose veins as well as the sufficiency or insufficiency of the Vv. perforantes.", "PMID": 964818}
{"id": "pubmed23n0032_6022", "title": "[Pharmacokinetics of angiographic contrast media].", "content": "The pharmaco-kinetics of angiographic contrast media in the extravascular space, which are largely unknown, were investigated experimentally in dogs. As part of a basic study, using radio-active contrast media, it was possible to determine the concentration and rate of elimination in practically all organs and tissues. Measurements were carried out first after prolonged infusion of the contrast under conditions of balanced flow, and secondly six hours after the end of the infusion. It was therefore possible to determine the inflow and loss of contrast medium in various organs or organ systems. The most commonly used angiographic contrast media in Germany were investigated. Their kinetic behaviour is largely identical, their pattern of distribution and elimination depended principally on the organ or tissue. In another study injections were carried out through angiographic catheters in a \"physiological angiographic\" manner. Measurements obtained show that distribution and excretion of the contrast medium are independent of the site of injection. The kinetics of the contrast within an organ do not differ, whether it is injected selectively or not. The presence of an entero-hepatic circulation of angiographic contrast media has been demonstrated, but quantitatively it is of little importance.", "contents": "[Pharmacokinetics of angiographic contrast media]. The pharmaco-kinetics of angiographic contrast media in the extravascular space, which are largely unknown, were investigated experimentally in dogs. As part of a basic study, using radio-active contrast media, it was possible to determine the concentration and rate of elimination in practically all organs and tissues. Measurements were carried out first after prolonged infusion of the contrast under conditions of balanced flow, and secondly six hours after the end of the infusion. It was therefore possible to determine the inflow and loss of contrast medium in various organs or organ systems. The most commonly used angiographic contrast media in Germany were investigated. Their kinetic behaviour is largely identical, their pattern of distribution and elimination depended principally on the organ or tissue. In another study injections were carried out through angiographic catheters in a \"physiological angiographic\" manner. Measurements obtained show that distribution and excretion of the contrast medium are independent of the site of injection. The kinetics of the contrast within an organ do not differ, whether it is injected selectively or not. The presence of an entero-hepatic circulation of angiographic contrast media has been demonstrated, but quantitatively it is of little importance.", "PMID": 964819}
{"id": "pubmed23n0032_6023", "title": "[Controversial caries prevention using fluorine].", "content": "Fluorine as an essential trace element is necessary for life. The artificial increase of the natural fluoride-content of drinking water up to a maximum of one milligram per litre has been proved a prophylactic step against dental caries. The available material is examined to find out how far side effects of this fluoride concentration as well as any higher fluoride doses can be brought in connection with special defects and patterns of diseases. A comprehensive review of scientific findings concerning physiological reactions caused by fluoride is given.", "contents": "[Controversial caries prevention using fluorine]. Fluorine as an essential trace element is necessary for life. The artificial increase of the natural fluoride-content of drinking water up to a maximum of one milligram per litre has been proved a prophylactic step against dental caries. The available material is examined to find out how far side effects of this fluoride concentration as well as any higher fluoride doses can be brought in connection with special defects and patterns of diseases. A comprehensive review of scientific findings concerning physiological reactions caused by fluoride is given.", "PMID": 964820}
{"id": "pubmed23n0032_6024", "title": "[Vascular malformations of the spinal cord. Diagnosis and therapy].", "content": "A review is given on the current diagnosis and treatment of spinal vascular malformations with special emphasis on the use of selective spinal angiography. Spinal vascular malformations are mostly encountered within the thoraco-lumbar segment of the spinal cord. Typical clinical symptoms are due to either progressive myelopathy or hemorrhage (subarachnoidal hemorrhage). The prognosis of untreated spinal angiomas is poor. Therefore, therapeutic measures are aiming to prevent bleeding of angiomas resulting in subarachnoidal hemorrhages or hematomyelia or to prevent spinal cord ischemia due to compression. Using selective spinal angiography, spinal angiomas can now be treated successfully by complete surgical extirpation or embolization procedures. The evaluation of radicular myelopathies and subarachnoidal hemorrhages of unknown origin has also considerably benefited from this technique. It is concluded that early diagnosis of spinal vascular malformations is of crucial importance for good therapeutical results.", "contents": "[Vascular malformations of the spinal cord. Diagnosis and therapy]. A review is given on the current diagnosis and treatment of spinal vascular malformations with special emphasis on the use of selective spinal angiography. Spinal vascular malformations are mostly encountered within the thoraco-lumbar segment of the spinal cord. Typical clinical symptoms are due to either progressive myelopathy or hemorrhage (subarachnoidal hemorrhage). The prognosis of untreated spinal angiomas is poor. Therefore, therapeutic measures are aiming to prevent bleeding of angiomas resulting in subarachnoidal hemorrhages or hematomyelia or to prevent spinal cord ischemia due to compression. Using selective spinal angiography, spinal angiomas can now be treated successfully by complete surgical extirpation or embolization procedures. The evaluation of radicular myelopathies and subarachnoidal hemorrhages of unknown origin has also considerably benefited from this technique. It is concluded that early diagnosis of spinal vascular malformations is of crucial importance for good therapeutical results.", "PMID": 964822}
{"id": "pubmed23n0032_6025", "title": "[New methods in vascular surgery. Intraoperative transluminal catheter-recanalization using Dotter's technic].", "content": "The combination of vascular operative procedures and transluminal recanalization with a Dotter-catheter is recommended. According to own results this has been proven to be very successful, especially in old patients or in patients in a reduced physical state. The main advantage is a shorter duration of the surgical procedures and a reduction of the operative stress situation compared to other surgical methods. For the application of the Dotter-technique, however, it is necessary to observe the indications carefully.", "contents": "[New methods in vascular surgery. Intraoperative transluminal catheter-recanalization using Dotter's technic]. The combination of vascular operative procedures and transluminal recanalization with a Dotter-catheter is recommended. According to own results this has been proven to be very successful, especially in old patients or in patients in a reduced physical state. The main advantage is a shorter duration of the surgical procedures and a reduction of the operative stress situation compared to other surgical methods. For the application of the Dotter-technique, however, it is necessary to observe the indications carefully.", "PMID": 964824}
{"id": "pubmed23n0032_6026", "title": "[Nodular fascitis].", "content": "Since 1971 four cases of Fasciitis nodularis were treated by the outpatient-department of the Surgical Clinic, Univ. of Erlangen. Alieno loco one patient's disease had been diagnosed as sarcoma, another patient had a lesion operated on which had been suggested being malignant by a pathologist. The danger in Fasciitis nodularis is, that it is easily considered a malignant process and therefore too radical a tumor therapy is performed. The differential diagnosis from clinical and histological point of view between Fasciitis nodularis and soft tissue tumors is presented.", "contents": "[Nodular fascitis]. Since 1971 four cases of Fasciitis nodularis were treated by the outpatient-department of the Surgical Clinic, Univ. of Erlangen. Alieno loco one patient's disease had been diagnosed as sarcoma, another patient had a lesion operated on which had been suggested being malignant by a pathologist. The danger in Fasciitis nodularis is, that it is easily considered a malignant process and therefore too radical a tumor therapy is performed. The differential diagnosis from clinical and histological point of view between Fasciitis nodularis and soft tissue tumors is presented.", "PMID": 964825}
{"id": "pubmed23n0032_6027", "title": "[Recurrent skin tumors and their surgical management].", "content": "Recurrences of basal cell carcinomas and squamous cell carcinomas are possible in cases of radiation before surgical treatment. This inadequacy occurs in general, if the emphasis of the treatment was on the cosmetical point and therefore the necessary radical therapy had been neglected. Furthermore cicatrizing basal cell carcinomas are prone to recurrences, after only a macroscopical tumor extirpation had been carried out. In special cases of epidermal tumor recurrences our dermato-surgical proceedings are demonstrated. The repair of large skin defects, caused by surgical treatment, requires methods of plastic and reconstructive surgery. Should there be any doubt whether the therapy is radical enough, material for microscopical examination should be taken immediately.", "contents": "[Recurrent skin tumors and their surgical management]. Recurrences of basal cell carcinomas and squamous cell carcinomas are possible in cases of radiation before surgical treatment. This inadequacy occurs in general, if the emphasis of the treatment was on the cosmetical point and therefore the necessary radical therapy had been neglected. Furthermore cicatrizing basal cell carcinomas are prone to recurrences, after only a macroscopical tumor extirpation had been carried out. In special cases of epidermal tumor recurrences our dermato-surgical proceedings are demonstrated. The repair of large skin defects, caused by surgical treatment, requires methods of plastic and reconstructive surgery. Should there be any doubt whether the therapy is radical enough, material for microscopical examination should be taken immediately.", "PMID": 964826}
{"id": "pubmed23n0032_6028", "title": "[Echocardiography].", "content": "Echocardiography makes possible the analysis of cardiac structures and their dynamics. The presence of valvular disease and its severity can be determined by evaluation of variations in the intensity of reflection and alterations in the motion of valvular structures. The echocardiographic representation of cardiac chambers permits the detection of congenital and acquired structural defects as well as an evaluation of ventricular function. Echocardiography provides a non-invasive means of diagnostic analysis throughout the spectrum of heart diseases and can replace a number of invasive diagnostic procedures.", "contents": "[Echocardiography]. Echocardiography makes possible the analysis of cardiac structures and their dynamics. The presence of valvular disease and its severity can be determined by evaluation of variations in the intensity of reflection and alterations in the motion of valvular structures. The echocardiographic representation of cardiac chambers permits the detection of congenital and acquired structural defects as well as an evaluation of ventricular function. Echocardiography provides a non-invasive means of diagnostic analysis throughout the spectrum of heart diseases and can replace a number of invasive diagnostic procedures.", "PMID": 964829}
{"id": "pubmed23n0032_6029", "title": "[Leading neurological symptoms of internal diseases].", "content": "After description of the central-nervous signs due to hypoglycemia, hypoxia, hypo- and hypercalcemia there are discussed several internal diseases with prominent neurologic symptoms like endocrine disorders of thyroid, adrenal and pituitary gland, malignant diseases especially regarding the hematopoietic system, disorders of the joints and connective tissue and metabolic disorders as acute intermittent porphyria, uremic and hepatic coma. The principal differential diagnostic considerations in this relation are being described in order to avoid severe diagnostic errors.", "contents": "[Leading neurological symptoms of internal diseases]. After description of the central-nervous signs due to hypoglycemia, hypoxia, hypo- and hypercalcemia there are discussed several internal diseases with prominent neurologic symptoms like endocrine disorders of thyroid, adrenal and pituitary gland, malignant diseases especially regarding the hematopoietic system, disorders of the joints and connective tissue and metabolic disorders as acute intermittent porphyria, uremic and hepatic coma. The principal differential diagnostic considerations in this relation are being described in order to avoid severe diagnostic errors.", "PMID": 964830}
{"id": "pubmed23n0032_6030", "title": "[Acute hepatic coma. Experimental study on the predictive value of clinical-chemical findings for the prognosis of acute hepatic coma].", "content": "In order to determine the validity of clinical-chemical parameters for the prognosis of hepatic failure, 28 pigs were subjected to liver ischemia for 40--160 minutes duration. The following parameters were studied: GOT, GPT, gamma-GT, LAP, LDH, GlDH, AP and isoenzymes, total bilirubin, potassium, sodium and chloride. In a statistical comparison in the surviving animals, an unexplainable increase in GlDH activity was observed. In the other clinical-chemical parameters none was seen to be of use for the prognosis for either life or death in acute hepatic failure.", "contents": "[Acute hepatic coma. Experimental study on the predictive value of clinical-chemical findings for the prognosis of acute hepatic coma]. In order to determine the validity of clinical-chemical parameters for the prognosis of hepatic failure, 28 pigs were subjected to liver ischemia for 40--160 minutes duration. The following parameters were studied: GOT, GPT, gamma-GT, LAP, LDH, GlDH, AP and isoenzymes, total bilirubin, potassium, sodium and chloride. In a statistical comparison in the surviving animals, an unexplainable increase in GlDH activity was observed. In the other clinical-chemical parameters none was seen to be of use for the prognosis for either life or death in acute hepatic failure.", "PMID": 964833}
{"id": "pubmed23n0032_6031", "title": "[Mycotoxins as a cause of disease in human beings].", "content": "Secondary metabolites, toxic to macro-organisms and micro-organisms, are produced by certain molds and some plant parasitic living fungi. A risk is given for man worldwide by ingestion or apparently also be other routes always undetected. In vivo-effects of the various mycotoxins are different, but mainly the liver is affected, expecially by the intake of smaller amounts of these poisons. Accordingly cirrhosis of the liver or primary liver carcinoma are expected in man as well as in animals and were already proved outside of Europe.", "contents": "[Mycotoxins as a cause of disease in human beings]. Secondary metabolites, toxic to macro-organisms and micro-organisms, are produced by certain molds and some plant parasitic living fungi. A risk is given for man worldwide by ingestion or apparently also be other routes always undetected. In vivo-effects of the various mycotoxins are different, but mainly the liver is affected, expecially by the intake of smaller amounts of these poisons. Accordingly cirrhosis of the liver or primary liver carcinoma are expected in man as well as in animals and were already proved outside of Europe.", "PMID": 964834}
{"id": "pubmed23n0032_6032", "title": "[Heart muscle after heart infarct. Function of surviving heart muscle following acute myocardial infarct].", "content": "In the initial phase of the infarction, there is a decrease of ventricular function due to loss of contractile activity. In addition, a negative effect of the paradoxical movement of the infarcted area on the hemodynamics of the ventricle is noted. The stiffening of the infarcted area in the early stage has a favorable influence on ventricular function, leading to a change in the elastic properties of the ventricle. The loss of ventricular compliance persists after the infarction, and its severity depends on the extent of myocardial destruction. In the non-compliant ventricle, the end-diastolic pressure rises without a proportionate increase in volume. Consequently, the ventricular function curve shows a shift downward and to the right, making it difficult to distinguish between the loss of contractile function or compliance in the heart in situ. Evaluation of the contractile properties of the surviving cardiac muscle in situ is, however, hardly possible due to the changed geometry and the additonal elastic elements functioning in series with the surviving muscle. To exclude these factors, a study of the contractile properties of the surviving cardiac muscle in the isolated state was carried out following experimental myocardial infarction in cats. By ligating several coronary branches, infarctions in the area of the left ventricle were caused; to avoid the ischaemic border zone of the infarction, right ventricular papillary muscles were studied. Haemodynamic investigations showed an increase in right ventricular end-diastolic pressure which persisted 6 weeks after infarction. As early as 48 hours but, more significantly 1 week after infarction, there was a decrease of actively developed force in the surviving cardiac muscle due to a lower rate of force development. The resting length tension curve of the surviving cardiac muscle after infarction showed no alterations; and 6 weeks following infarction, almost normal contractility parameters were observed. As a result of the infarction, a decrease in contractility in the surviving cardiac muscle is observed during the early stage, which regresses after complete recuperation.", "contents": "[Heart muscle after heart infarct. Function of surviving heart muscle following acute myocardial infarct]. In the initial phase of the infarction, there is a decrease of ventricular function due to loss of contractile activity. In addition, a negative effect of the paradoxical movement of the infarcted area on the hemodynamics of the ventricle is noted. The stiffening of the infarcted area in the early stage has a favorable influence on ventricular function, leading to a change in the elastic properties of the ventricle. The loss of ventricular compliance persists after the infarction, and its severity depends on the extent of myocardial destruction. In the non-compliant ventricle, the end-diastolic pressure rises without a proportionate increase in volume. Consequently, the ventricular function curve shows a shift downward and to the right, making it difficult to distinguish between the loss of contractile function or compliance in the heart in situ. Evaluation of the contractile properties of the surviving cardiac muscle in situ is, however, hardly possible due to the changed geometry and the additonal elastic elements functioning in series with the surviving muscle. To exclude these factors, a study of the contractile properties of the surviving cardiac muscle in the isolated state was carried out following experimental myocardial infarction in cats. By ligating several coronary branches, infarctions in the area of the left ventricle were caused; to avoid the ischaemic border zone of the infarction, right ventricular papillary muscles were studied. Haemodynamic investigations showed an increase in right ventricular end-diastolic pressure which persisted 6 weeks after infarction. As early as 48 hours but, more significantly 1 week after infarction, there was a decrease of actively developed force in the surviving cardiac muscle due to a lower rate of force development. The resting length tension curve of the surviving cardiac muscle after infarction showed no alterations; and 6 weeks following infarction, almost normal contractility parameters were observed. As a result of the infarction, a decrease in contractility in the surviving cardiac muscle is observed during the early stage, which regresses after complete recuperation.", "PMID": 964835}
{"id": "pubmed23n0032_6033", "title": "[Value of results of animal experiments for the evaluation of drugs. 3. Animal trials and subjective probability].", "content": "Inasmuch as the findings in pharmacology and toxicology are based on animal trials these disciplines have to be considered as subjective sciences, since the experimentally obtained objective data become medically relevant only on the basis of subjective probabilities. The value of pharmacological and toxicological tests is dependent on the quality of the investigator. Data obtained in animals trials are of no relevance for human medicine unless the investigator substantiates his subjective probabilities and at the same time demonstrates how the hypotheses won in animal trials can be verified in man. Toxicological testing methods allowing the \"preselection\" of drugs or any other kind of deductions without verification in man do not exist. Animal studies which are not based on the principles of decision theory serve merely as an alibi and may lead to wrong conclusions. Besides, they interfere with the German Animal Protection Law. Methodological problems can neither be solved by normative regulations of an administrative authority nor by increasing experimental activity.", "contents": "[Value of results of animal experiments for the evaluation of drugs. 3. Animal trials and subjective probability]. Inasmuch as the findings in pharmacology and toxicology are based on animal trials these disciplines have to be considered as subjective sciences, since the experimentally obtained objective data become medically relevant only on the basis of subjective probabilities. The value of pharmacological and toxicological tests is dependent on the quality of the investigator. Data obtained in animals trials are of no relevance for human medicine unless the investigator substantiates his subjective probabilities and at the same time demonstrates how the hypotheses won in animal trials can be verified in man. Toxicological testing methods allowing the \"preselection\" of drugs or any other kind of deductions without verification in man do not exist. Animal studies which are not based on the principles of decision theory serve merely as an alibi and may lead to wrong conclusions. Besides, they interfere with the German Animal Protection Law. Methodological problems can neither be solved by normative regulations of an administrative authority nor by increasing experimental activity.", "PMID": 964836}
{"id": "pubmed23n0032_6034", "title": "[The malignant struma. Pathological anatomy and functional criteria].", "content": "Description of the pathological anatomy of thyroid cancer with classification into carcinomas, sarcomas and special forms of tumors. Concerning the carcinomas of the thyroid gland, the malignant papilloma (papillary carcinoma) and the medullary amyloid- and calcitonin-producing carcinoma deserve special consideration because of their small malignancy and therefore favourable prognosis. Also the proliferating struma Langhans does not come up to \"real\" carcinomas (organoid structure of the tumor tissue) concerning the growing speed. All other types of carcinomas, as well as sarcomas, are fast growing malignant tumors, which also place metastases early. The metastasizing struma-adenoma is not a malignant tumor in the sense of the term. In addition, it is necessary to refer to the existing difficulties in marking off clearly structurally sarcomatous tumors of the thyroid gland from carcinomas. Besides, one must emphasize, that thyreostatic substances, also ionizing rays (i.e. radioiodine) can produce considerable tissue-changes with cell polymorphia and that a considerable increase in the number of large-cell eosinophilic adenomas (oncocytomas, H\u00fcrthle-cell adenomas) exists. The sclerosizing microcarcinoma (Graham's tumor) is not a carcinoma in the sense of the term.", "contents": "[The malignant struma. Pathological anatomy and functional criteria]. Description of the pathological anatomy of thyroid cancer with classification into carcinomas, sarcomas and special forms of tumors. Concerning the carcinomas of the thyroid gland, the malignant papilloma (papillary carcinoma) and the medullary amyloid- and calcitonin-producing carcinoma deserve special consideration because of their small malignancy and therefore favourable prognosis. Also the proliferating struma Langhans does not come up to \"real\" carcinomas (organoid structure of the tumor tissue) concerning the growing speed. All other types of carcinomas, as well as sarcomas, are fast growing malignant tumors, which also place metastases early. The metastasizing struma-adenoma is not a malignant tumor in the sense of the term. In addition, it is necessary to refer to the existing difficulties in marking off clearly structurally sarcomatous tumors of the thyroid gland from carcinomas. Besides, one must emphasize, that thyreostatic substances, also ionizing rays (i.e. radioiodine) can produce considerable tissue-changes with cell polymorphia and that a considerable increase in the number of large-cell eosinophilic adenomas (oncocytomas, H\u00fcrthle-cell adenomas) exists. The sclerosizing microcarcinoma (Graham's tumor) is not a carcinoma in the sense of the term.", "PMID": 964839}
{"id": "pubmed23n0032_6035", "title": "[Retinal hemangiomas].", "content": "In the present study the clinical picture, therapy and pathogenesis of 6 retinal diseases are individually described and discussed. These are: familial tortuosity of the small retinal arteries, arteriovenous haemangioma, cavernous haemangioma, Leber's multiple retinal aneurysms, Coats' disease and von Hippel's haemangiomatosis retinae. They can all be subsumed under the broad concept of haemangioma and represent hamartias and hamartomas of the retinal vascular system.", "contents": "[Retinal hemangiomas]. In the present study the clinical picture, therapy and pathogenesis of 6 retinal diseases are individually described and discussed. These are: familial tortuosity of the small retinal arteries, arteriovenous haemangioma, cavernous haemangioma, Leber's multiple retinal aneurysms, Coats' disease and von Hippel's haemangiomatosis retinae. They can all be subsumed under the broad concept of haemangioma and represent hamartias and hamartomas of the retinal vascular system.", "PMID": 964840}
{"id": "pubmed23n0032_6036", "title": "[Clinical experiences with vasoatherolip in early cerebral insufficiency].", "content": "Under clinical conditions and during 12 weeks Vasoatherolip (alufibrat and nicotinuric acid) were administered to a group of 30 patients suffering from definitive symptoms of early cerebral insufficiency. The daily dosage was 3 X 1 tablet. In about 66 per cent of the patients a significant improvement, especially for intellectual and affective capacity was achieved. This could be proved by a number of confirmed psychometrical tests (i.e. concentration-test, Benton-test, pegboard-test, Wechsler-test, etc.).", "contents": "[Clinical experiences with vasoatherolip in early cerebral insufficiency]. Under clinical conditions and during 12 weeks Vasoatherolip (alufibrat and nicotinuric acid) were administered to a group of 30 patients suffering from definitive symptoms of early cerebral insufficiency. The daily dosage was 3 X 1 tablet. In about 66 per cent of the patients a significant improvement, especially for intellectual and affective capacity was achieved. This could be proved by a number of confirmed psychometrical tests (i.e. concentration-test, Benton-test, pegboard-test, Wechsler-test, etc.).", "PMID": 964843}
{"id": "pubmed23n0032_6037", "title": "[Functional scintigraphy of the heat in congenital or acquired heart valve diseases].", "content": "Quantitative nuclear cardiography was applied to 400 patients with valvular heart disease proven by heart catheterisation. The study yielded the following results: 1. The parameters used were directly related to th4 cardiac index, the enddiastolic volume of the left ventricle and the pressures in the pulmonary vascular system. 2. The method proved to be highly sensitive in stating whether there was a hemodynamically effective valvular disease present or not. Differential diagnosis, however, was not possible. 3. The prolongation of the circulation times correlated with the hemodynamic severity of the disease. 4. The method could be used in evaluation of corrective surgery and for follow-up after operation.", "contents": "[Functional scintigraphy of the heat in congenital or acquired heart valve diseases]. Quantitative nuclear cardiography was applied to 400 patients with valvular heart disease proven by heart catheterisation. The study yielded the following results: 1. The parameters used were directly related to th4 cardiac index, the enddiastolic volume of the left ventricle and the pressures in the pulmonary vascular system. 2. The method proved to be highly sensitive in stating whether there was a hemodynamically effective valvular disease present or not. Differential diagnosis, however, was not possible. 3. The prolongation of the circulation times correlated with the hemodynamic severity of the disease. 4. The method could be used in evaluation of corrective surgery and for follow-up after operation.", "PMID": 964846}
{"id": "pubmed23n0032_6038", "title": "[Side-effects of oral contraceptives under psychological-sociological aspects. Results of an empirical study on 345 women].", "content": "Do women without experience in taking oral contraceptives expect more side-effects than women, who use this contraceptive method, ever experienced? This question was examined on 345 women in pregnancy and childbed Psychological and sociological data, as personality factors, general well-being, school education, intelligence and age  were evaluated. The attitude towards the pill depended on the families opinion of the pill, fear of cancer, knowledge about the pill and experience of menstruation. Half of the women had never taken the pill. Those women expected more side-effects than women, who had taken the pill had experienced (p less than 0,05). Those women who had never taken the pill stated frequently, that their partner (p less than 0,001) and mother (p less than 0,001) rejected the pill. Three fourth of women without experiences in taking the pill doubted thepill to be safe. Two thirds of these women were anxious the pill might cause cancer. Four fifth of the questioned women thought a pregnancy would mean more stress than taking the pill, women without experience in taking the pill more frequently thought the pill to be more disturbing (p less than 0,01). Women who had never taken the pill, suffered more often from reduced well-being (p less than 0,05) and were also more often introverted (p less than 0,1). The more irregular and painful menstruations were, the more women accepted the pill (p less than 0,01). Women with experience in taking the pill had often higher intelligence (p less than 0,05) and a higher education level (p less than 0,01). This is important for the interpretation of the results. The results were statistically checked by the x2-method.", "contents": "[Side-effects of oral contraceptives under psychological-sociological aspects. Results of an empirical study on 345 women]. Do women without experience in taking oral contraceptives expect more side-effects than women, who use this contraceptive method, ever experienced? This question was examined on 345 women in pregnancy and childbed Psychological and sociological data, as personality factors, general well-being, school education, intelligence and age  were evaluated. The attitude towards the pill depended on the families opinion of the pill, fear of cancer, knowledge about the pill and experience of menstruation. Half of the women had never taken the pill. Those women expected more side-effects than women, who had taken the pill had experienced (p less than 0,05). Those women who had never taken the pill stated frequently, that their partner (p less than 0,001) and mother (p less than 0,001) rejected the pill. Three fourth of women without experiences in taking the pill doubted thepill to be safe. Two thirds of these women were anxious the pill might cause cancer. Four fifth of the questioned women thought a pregnancy would mean more stress than taking the pill, women without experience in taking the pill more frequently thought the pill to be more disturbing (p less than 0,01). Women who had never taken the pill, suffered more often from reduced well-being (p less than 0,05) and were also more often introverted (p less than 0,1). The more irregular and painful menstruations were, the more women accepted the pill (p less than 0,01). Women with experience in taking the pill had often higher intelligence (p less than 0,05) and a higher education level (p less than 0,01). This is important for the interpretation of the results. The results were statistically checked by the x2-method.", "PMID": 964848}
{"id": "pubmed23n0032_6039", "title": "[Vena cava occlusion syndrome. Cardiovascular parameters and uterine blood supply].", "content": "In late pregnancy about 10% of the patients suffer from dizziness and a shock symptomatic if placed in a supine position. These symptoms are reversible if the patient turns to the lateral position. In the present paper experiments in human and sheep are reviewed. The cause for this disease is the occlusion of the inferior vena cava by the gravid uterus. The venous backflow to the maternal heart is reduced and the cardiac output decreased by about 1 1/min. The total peripheral resistance (TPR) is increased (Lees et al. 1967b). The increase in TPR is due to the compression of the vena cava by the uterus and not related to an increased arterial tone during the first time course. The fall in mean arterial blood pressure is of varying degree and paralleled by an increase of the blood pressure in the vena cava below the compression and the uterine vein. The fall of the mean arterial blood pressure war related to the decrease of the perfusion pressure. The fall in arterial blood pressure and most important the rise of the uterine vein blood pressure led to a decrease of the perfusion pressure across the uterus. The uterine blood flow fell proportional to the drop of perfusion pressure. The uterine vasculare resistance remained unchanged. The fall of uterine blood flow was associated with a fall of fetal heart rate.", "contents": "[Vena cava occlusion syndrome. Cardiovascular parameters and uterine blood supply]. In late pregnancy about 10% of the patients suffer from dizziness and a shock symptomatic if placed in a supine position. These symptoms are reversible if the patient turns to the lateral position. In the present paper experiments in human and sheep are reviewed. The cause for this disease is the occlusion of the inferior vena cava by the gravid uterus. The venous backflow to the maternal heart is reduced and the cardiac output decreased by about 1 1/min. The total peripheral resistance (TPR) is increased (Lees et al. 1967b). The increase in TPR is due to the compression of the vena cava by the uterus and not related to an increased arterial tone during the first time course. The fall in mean arterial blood pressure is of varying degree and paralleled by an increase of the blood pressure in the vena cava below the compression and the uterine vein. The fall of the mean arterial blood pressure war related to the decrease of the perfusion pressure. The fall in arterial blood pressure and most important the rise of the uterine vein blood pressure led to a decrease of the perfusion pressure across the uterus. The uterine blood flow fell proportional to the drop of perfusion pressure. The uterine vasculare resistance remained unchanged. The fall of uterine blood flow was associated with a fall of fetal heart rate.", "PMID": 964849}
{"id": "pubmed23n0032_6040", "title": "[Indications for vital and preventive cesarean section].", "content": "The indications for Caesarean section have not only increased but also changed. A spectrum of indications is described which comprises emergency and preventive indications. The postpartal state of the newborn after Caesarean section (200 cases) is described regarding indication, 148 additional cases due to the change of indication. The lowest Apgar-scores were found in newborns after sectiones for emergency fetal indications. ithe increase of the Caesarean section rate since 1971 was due to preventive fetal indications, which reached in the last 2 years more than 50%. It was found that the \"\"Caesarean section infant'' is not endangered, but the infant delivered by Caesarean section for reasons of emergency indications of fetal or maternal nature. Control examinations during pregnancy should help to recognize the risk-pregnancy and to decide on necessary ambulant treatment or hospitalisation.", "contents": "[Indications for vital and preventive cesarean section]. The indications for Caesarean section have not only increased but also changed. A spectrum of indications is described which comprises emergency and preventive indications. The postpartal state of the newborn after Caesarean section (200 cases) is described regarding indication, 148 additional cases due to the change of indication. The lowest Apgar-scores were found in newborns after sectiones for emergency fetal indications. ithe increase of the Caesarean section rate since 1971 was due to preventive fetal indications, which reached in the last 2 years more than 50%. It was found that the \"\"Caesarean section infant'' is not endangered, but the infant delivered by Caesarean section for reasons of emergency indications of fetal or maternal nature. Control examinations during pregnancy should help to recognize the risk-pregnancy and to decide on necessary ambulant treatment or hospitalisation.", "PMID": 964850}
{"id": "pubmed23n0032_6041", "title": "[Changes in the electrolyte and acid-base equilibrium during the first days of life].", "content": "The concentrations of potassium, sodium and magnesium in plasma, red corpuscels and acid-base status were measured during the first five days of life. Significant differences were observed during this period of  life, these values also differ markedly from those of adults. The causes of the particular conditions of the neonates are complex and not all known at present.", "contents": "[Changes in the electrolyte and acid-base equilibrium during the first days of life]. The concentrations of potassium, sodium and magnesium in plasma, red corpuscels and acid-base status were measured during the first five days of life. Significant differences were observed during this period of  life, these values also differ markedly from those of adults. The causes of the particular conditions of the neonates are complex and not all known at present.", "PMID": 964851}
{"id": "pubmed23n0032_6042", "title": "[Impulse cytophotometry in neoplasm prevention. The behavior of normal vaginal smears in the impulse cytophotometric histogram].", "content": "Evaluation of histograms of normal cervical and vaginal smears, produced by \"\"rapid flow through'' cytophotometry. Normal cytological cervical and vaginal smears were examined for their appearance in histograms in order to find criteria for the classification of \"\"normal non-pathological'' cytological slides. Simultaneously, the reliability of the standardized method for preparation of vaginal and cervical smears was checked. The diagrams and data produced by the histograph were also coded on line and analyzed by a computer; thus obtaining an objective mathematical analysis and avoiding errors due to subjective visual analysis of the histograms. The objective interpretation of histograms of normal vaginal smears is an important step to the realisation of automatic pre-screening.", "contents": "[Impulse cytophotometry in neoplasm prevention. The behavior of normal vaginal smears in the impulse cytophotometric histogram]. Evaluation of histograms of normal cervical and vaginal smears, produced by \"\"rapid flow through'' cytophotometry. Normal cytological cervical and vaginal smears were examined for their appearance in histograms in order to find criteria for the classification of \"\"normal non-pathological'' cytological slides. Simultaneously, the reliability of the standardized method for preparation of vaginal and cervical smears was checked. The diagrams and data produced by the histograph were also coded on line and analyzed by a computer; thus obtaining an objective mathematical analysis and avoiding errors due to subjective visual analysis of the histograms. The objective interpretation of histograms of normal vaginal smears is an important step to the realisation of automatic pre-screening.", "PMID": 964854}
{"id": "pubmed23n0032_6043", "title": "[A reducing diet with a high proportion of bread].", "content": "10 female and 10 male moderately obese persons, pursuing their usual activities, received a reduction diet for 4 weeks, in which 46% of the calories were supplied by rye bread. In percent of calories the diet consisted of 60% carbohydrates, 20% fat and 20% protein. The total intake of calories corresponded to 60% of the caloric requirement. Weight losses ranged from 1.8 to 10.7 kg; the mean was 6.0 kg. Serum cholesterol values tended to decrease by 15% on the average, triglyceride values decreased by 38%. All subjects declared to be satiated during the entire 4 week period. No significant differences between the influence of crumb and crust on weight reduction could be observed.", "contents": "[A reducing diet with a high proportion of bread]. 10 female and 10 male moderately obese persons, pursuing their usual activities, received a reduction diet for 4 weeks, in which 46% of the calories were supplied by rye bread. In percent of calories the diet consisted of 60% carbohydrates, 20% fat and 20% protein. The total intake of calories corresponded to 60% of the caloric requirement. Weight losses ranged from 1.8 to 10.7 kg; the mean was 6.0 kg. Serum cholesterol values tended to decrease by 15% on the average, triglyceride values decreased by 38%. All subjects declared to be satiated during the entire 4 week period. No significant differences between the influence of crumb and crust on weight reduction could be observed.", "PMID": 964855}
{"id": "pubmed23n0032_6044", "title": "[The pathogenesis of multiple sclerosis. Cytotoxic antibodies against myelin sheath tissue in multiple sclerosis].", "content": "Cytotoxic antibodies to myelin can be demonstrated by the method of 51Cr-release from chick erythrocytes coated with myelin basic protein. The cytotoxic antibody is inactivated by heating to 56 degrees C and needs complement in order to exert its action. The antibody was determined as IgM and IgG. It has relative specifity and shows cross-reaction with other basic proteins. The cytotoxic antibody was found in only 8% of healthy persons. Patients with multiple sclerosis were positive in 87% of the cases and in acute cases in 94%. In other neurological diseases cytotoxic antibody was present in 64%. The occurrence of cytotoxic antibody to myelin protein is not specific for a particular neurological disorder, especially not for multiple sclerosis. Cytotoxic antibodies arise as a secondary phenomenon, they are not the cause of the disease involved. They appear to be suitable, however, to determine, in association with cellular immunological reactions against myelin which may be regarded as the \"primary\" immunological processes, the demyelination process in the multiple sclerosis focus.", "contents": "[The pathogenesis of multiple sclerosis. Cytotoxic antibodies against myelin sheath tissue in multiple sclerosis]. Cytotoxic antibodies to myelin can be demonstrated by the method of 51Cr-release from chick erythrocytes coated with myelin basic protein. The cytotoxic antibody is inactivated by heating to 56 degrees C and needs complement in order to exert its action. The antibody was determined as IgM and IgG. It has relative specifity and shows cross-reaction with other basic proteins. The cytotoxic antibody was found in only 8% of healthy persons. Patients with multiple sclerosis were positive in 87% of the cases and in acute cases in 94%. In other neurological diseases cytotoxic antibody was present in 64%. The occurrence of cytotoxic antibody to myelin protein is not specific for a particular neurological disorder, especially not for multiple sclerosis. Cytotoxic antibodies arise as a secondary phenomenon, they are not the cause of the disease involved. They appear to be suitable, however, to determine, in association with cellular immunological reactions against myelin which may be regarded as the \"primary\" immunological processes, the demyelination process in the multiple sclerosis focus.", "PMID": 964856}
{"id": "pubmed23n0032_6045", "title": "[Stimulation of the bodies defense against cervix carcinoma. Clinical-experimental study].", "content": "The method of choice for stimulation is extracorporeal radiotherapy of the blood, which has undergone stepwise clinical experimental development, is recommended. Implementation of this method induces a marked increase in the biosynthesis of certain serum proteins, especially the IgM and IgG fractions of the gammaglobulins and there is a definite tendency to normalization of certain serum enzymes in patients with cervical carcinoma. The dose dependence observed in the present study and the good clinical tolerance shown to this radiotherapeutic procedure are emphasized. The investigation is concluded with a cautions appraisal of the possible mode of action of extracorporeal X ray-irradiation of the blood at a dosage of 5000 R/O.", "contents": "[Stimulation of the bodies defense against cervix carcinoma. Clinical-experimental study]. The method of choice for stimulation is extracorporeal radiotherapy of the blood, which has undergone stepwise clinical experimental development, is recommended. Implementation of this method induces a marked increase in the biosynthesis of certain serum proteins, especially the IgM and IgG fractions of the gammaglobulins and there is a definite tendency to normalization of certain serum enzymes in patients with cervical carcinoma. The dose dependence observed in the present study and the good clinical tolerance shown to this radiotherapeutic procedure are emphasized. The investigation is concluded with a cautions appraisal of the possible mode of action of extracorporeal X ray-irradiation of the blood at a dosage of 5000 R/O.", "PMID": 964857}
{"id": "pubmed23n0032_6046", "title": "[Infant nutrition in West Africa].", "content": "The breastfeeding-customs of Westafrican tribes are described. Details are given about the duration of the suckling-period, about the nourishment of the baby after breastfeeding has been reduced and about the application of indigenous lactogenic substances. The relations between mother and child as well as the relations between wife and husband during the suckling-period are illustrated; the dangers of a precocious weaning from breastfeeding and the dangers due to the application of wrongly prepared bottle-milk are discussed.", "contents": "[Infant nutrition in West Africa]. The breastfeeding-customs of Westafrican tribes are described. Details are given about the duration of the suckling-period, about the nourishment of the baby after breastfeeding has been reduced and about the application of indigenous lactogenic substances. The relations between mother and child as well as the relations between wife and husband during the suckling-period are illustrated; the dangers of a precocious weaning from breastfeeding and the dangers due to the application of wrongly prepared bottle-milk are discussed.", "PMID": 964860}
{"id": "pubmed23n0032_6047", "title": "[Juvenile psychogenic hearing disorders].", "content": "Psychogenic hearing problems exist even in childhood. They must be differentiated from the stimulated or aggravated hearing losses. A characteristic is the discrepancy between the understanding during conversation and the bad results of the hearing tests. The psychical case history of the patient shows his conflict-situation and personal problems. If the psychogenic hearing problems do not improve on their own or by means of oto-rhinolaryngical placebo-therapy, psycho-therapy will be needed. Taking the example of 2 children, where the suspected primary diagnosis was sudden deafness, the problems of diagnosis was shown.", "contents": "[Juvenile psychogenic hearing disorders]. Psychogenic hearing problems exist even in childhood. They must be differentiated from the stimulated or aggravated hearing losses. A characteristic is the discrepancy between the understanding during conversation and the bad results of the hearing tests. The psychical case history of the patient shows his conflict-situation and personal problems. If the psychogenic hearing problems do not improve on their own or by means of oto-rhinolaryngical placebo-therapy, psycho-therapy will be needed. Taking the example of 2 children, where the suspected primary diagnosis was sudden deafness, the problems of diagnosis was shown.", "PMID": 964861}
{"id": "pubmed23n0032_6048", "title": "[Endoscopy, a hobby for specialists--or more?].", "content": "In the years between 1958 and 1970, diagnostic endoscopy made decisive advances. In this period, the ground was prepared for operative endoscopy. This applies not only to the technical, but also to the psychological aspects. The fact that formerly hidden structures could now be directly inspected and \"\"grasped'', spurned the endoscopist to intensive activity. Polypectomy, papillotomy with the removal of gallstones and, increasingly, photocoagulation for the arrest of bleeding, have made in many cases classical surgical operations superfluous. In close cooperation and rapport with the surgeons, the indications for, but also the limitations of, the new procedure are being established. In addition to the more practical aspects, modern endoscopy has also created a bridge to better understanding of biochemical processes in the gastrointestinal tract. The possibility of being able to investigate the effects of newly discovered, analysed and synthesized gastrointestinal hormones has been considerably improved. Endoscopy, formerly considered, more or less, a hobby for a few specialists, has become a sort of intermediary system which is capable of providing excellent diagnostic information, of making possible elegant therapeutic measures and of producing new biochemical information. In this creative function, it is surpassed by virtually no other method. A pre-condition for this development was, however, that the endoscopist was not content to remain merely an observer of interesting views, but was, rather, an active physician, to whom the advantages to the patient of early detection of disease and suitable therapeutic measures were equally important as the useful information obtained on biochemical relationships.", "contents": "[Endoscopy, a hobby for specialists--or more?]. In the years between 1958 and 1970, diagnostic endoscopy made decisive advances. In this period, the ground was prepared for operative endoscopy. This applies not only to the technical, but also to the psychological aspects. The fact that formerly hidden structures could now be directly inspected and \"\"grasped'', spurned the endoscopist to intensive activity. Polypectomy, papillotomy with the removal of gallstones and, increasingly, photocoagulation for the arrest of bleeding, have made in many cases classical surgical operations superfluous. In close cooperation and rapport with the surgeons, the indications for, but also the limitations of, the new procedure are being established. In addition to the more practical aspects, modern endoscopy has also created a bridge to better understanding of biochemical processes in the gastrointestinal tract. The possibility of being able to investigate the effects of newly discovered, analysed and synthesized gastrointestinal hormones has been considerably improved. Endoscopy, formerly considered, more or less, a hobby for a few specialists, has become a sort of intermediary system which is capable of providing excellent diagnostic information, of making possible elegant therapeutic measures and of producing new biochemical information. In this creative function, it is surpassed by virtually no other method. A pre-condition for this development was, however, that the endoscopist was not content to remain merely an observer of interesting views, but was, rather, an active physician, to whom the advantages to the patient of early detection of disease and suitable therapeutic measures were equally important as the useful information obtained on biochemical relationships.", "PMID": 964864}
{"id": "pubmed23n0032_6049", "title": "[The use of vaginal tampons in hospital and practice].", "content": "Industrially manufactured cotton wool tampons have been used for 5 years on approx. 5,000 women. The tampons were used in cases of inflammatory diseases of the vagina and of the cervix as well as postoperatively after abdominal and vaginal operations in order to apply medicaments. The absorbing effect of the tampons immediately stops the disagreeable secretion. The insertion of the tampons is simple and can be performed by the women themselves. Use of the pre-fabricated tampons is more economical than that of those tampons developed by our personnel. The advantages of the tampons during menstruation as well as during sports activities while menstruating are discussed. No side effects were observed during tampon use provided the tampons were inserted correctly.", "contents": "[The use of vaginal tampons in hospital and practice]. Industrially manufactured cotton wool tampons have been used for 5 years on approx. 5,000 women. The tampons were used in cases of inflammatory diseases of the vagina and of the cervix as well as postoperatively after abdominal and vaginal operations in order to apply medicaments. The absorbing effect of the tampons immediately stops the disagreeable secretion. The insertion of the tampons is simple and can be performed by the women themselves. Use of the pre-fabricated tampons is more economical than that of those tampons developed by our personnel. The advantages of the tampons during menstruation as well as during sports activities while menstruating are discussed. No side effects were observed during tampon use provided the tampons were inserted correctly.", "PMID": 964867}
{"id": "pubmed23n0032_6050", "title": "[Histamine-H2-receptor antagonists. Physiological and clinical aspects].", "content": "Histamine-H2-receptor antagonists (Burimamide, Metiamide, Cimetidine) may inhibit histamine-stimulated gastric secretion in various species including man; in constrast, conventional antihistaminics (H1-antagonists) do not do so. Gastric secretion as stimulated by pentagastrin, insulin, 2-deoxy-glucose or an intragastrically instilled meal is also decreased by H2-antagonists, and a final common mediator role for histamine in the gastric secretory is discussed. H2-antagonists do also inhibit pancreatic enzyme secretion, but do not affect at all bicarbonate secretion. Recent clinical trials have shown Metiamide to be superior to placebo in therapy of duodenal ulcer.", "contents": "[Histamine-H2-receptor antagonists. Physiological and clinical aspects]. Histamine-H2-receptor antagonists (Burimamide, Metiamide, Cimetidine) may inhibit histamine-stimulated gastric secretion in various species including man; in constrast, conventional antihistaminics (H1-antagonists) do not do so. Gastric secretion as stimulated by pentagastrin, insulin, 2-deoxy-glucose or an intragastrically instilled meal is also decreased by H2-antagonists, and a final common mediator role for histamine in the gastric secretory is discussed. H2-antagonists do also inhibit pancreatic enzyme secretion, but do not affect at all bicarbonate secretion. Recent clinical trials have shown Metiamide to be superior to placebo in therapy of duodenal ulcer.", "PMID": 964868}
{"id": "pubmed23n0032_6051", "title": "[Preventive medicine aspects in the sex education of young girls].", "content": "Girls in their teens have many questions and uncertainities regarding their physical maturation. In many cases personal hygiene is insufficient, resulting from lack of information and education in the family. The onset of menarche introduces a new dimension of life to cope with. Competent advice is essential in these habit-forming years to correct misconceptions and lead to proper health care.", "contents": "[Preventive medicine aspects in the sex education of young girls]. Girls in their teens have many questions and uncertainities regarding their physical maturation. In many cases personal hygiene is insufficient, resulting from lack of information and education in the family. The onset of menarche introduces a new dimension of life to cope with. Competent advice is essential in these habit-forming years to correct misconceptions and lead to proper health care.", "PMID": 964876}
{"id": "pubmed23n0032_6052", "title": "[Diagnosis of hydrocephalus. Changes in visual evoked potentials in children with progressive hydrocephalus internus].", "content": "Visual evoked potentials (VEP) of 12 children with progressive hydrocephalus internus were studied. Depending on the severity of hydrocephalus internus VEP show remarkable changes. First of deformation of VEP occurs, than a slowing of latencies and finally an extreme increase of latencies combined with a further deformation of VEP. The whole duration of the VEP shows a marked prolongation.", "contents": "[Diagnosis of hydrocephalus. Changes in visual evoked potentials in children with progressive hydrocephalus internus]. Visual evoked potentials (VEP) of 12 children with progressive hydrocephalus internus were studied. Depending on the severity of hydrocephalus internus VEP show remarkable changes. First of deformation of VEP occurs, than a slowing of latencies and finally an extreme increase of latencies combined with a further deformation of VEP. The whole duration of the VEP shows a marked prolongation.", "PMID": 964877}
{"id": "pubmed23n0032_6053", "title": "[Folk medicine, experience therapy, a component of modern therapeutics?].", "content": "Empirical medicine has three main supporting pillars: physical treatment, dietetics and phytotherapy. In this area of medicine, a number of therapeutical methods have been evolved which stand up to investigations employing scientific criteria, and which, in consequence, have become officially recognized by medical science. Examples are: diet rich in bulkage, digitalis, products of Papaver somniferum (Poppy) and salicylates. The acceptance of phytotherapeutic agents into the drug armamentarium of scientific medicine presupposes the availability of test methods suitable to demonstrate their effectiveness. Quite a number of remedies that have always been firmly anchored in empirical medicine, but which was applied regularly only by the nature healer, have suddenly, in the light of new test methods, been shown to be effective medicines; thus, for example, onions and garlic are good for thrombotic processes and hyperglycaemia, carminatives for sphincter spasms in the gastrointestinal tract, and alcohol is an appetite-stimulating stomachic. This fact, the limited applicability of the information obtained from animal experiments, and the further fact that even test results obtained in human subjects cannot be applied on a world-wide basis, exhort us to take care not to subscribe to an all-too apodictic classification of therapeutic measures into effective and noneffective. A further point for consideration is that the administration even of what is only a supposed remedy, can trigger or promote healing simply on the strength of the action of the physician. Scientific thinking is an indispensible precondition of the physician's activity--to consider it as the beginning and end of all things medical, however, is simply to demonstrate a narrowness of outlook.", "contents": "[Folk medicine, experience therapy, a component of modern therapeutics?]. Empirical medicine has three main supporting pillars: physical treatment, dietetics and phytotherapy. In this area of medicine, a number of therapeutical methods have been evolved which stand up to investigations employing scientific criteria, and which, in consequence, have become officially recognized by medical science. Examples are: diet rich in bulkage, digitalis, products of Papaver somniferum (Poppy) and salicylates. The acceptance of phytotherapeutic agents into the drug armamentarium of scientific medicine presupposes the availability of test methods suitable to demonstrate their effectiveness. Quite a number of remedies that have always been firmly anchored in empirical medicine, but which was applied regularly only by the nature healer, have suddenly, in the light of new test methods, been shown to be effective medicines; thus, for example, onions and garlic are good for thrombotic processes and hyperglycaemia, carminatives for sphincter spasms in the gastrointestinal tract, and alcohol is an appetite-stimulating stomachic. This fact, the limited applicability of the information obtained from animal experiments, and the further fact that even test results obtained in human subjects cannot be applied on a world-wide basis, exhort us to take care not to subscribe to an all-too apodictic classification of therapeutic measures into effective and noneffective. A further point for consideration is that the administration even of what is only a supposed remedy, can trigger or promote healing simply on the strength of the action of the physician. Scientific thinking is an indispensible precondition of the physician's activity--to consider it as the beginning and end of all things medical, however, is simply to demonstrate a narrowness of outlook.", "PMID": 964878}
{"id": "pubmed23n0032_6054", "title": "[Emergency situations in drug addicts. Classification, diagnostic and therapeutic measures].", "content": "In drug addicts emergency situations can occur which differ only slightly in symptoms but require different therapeutic measures, depending on type of the drug and symptomatology. Diagnostic and therapeutical difficulties in emergency situations, especially the not unfrequent late complications and the considerable danger of suicidal actions require in general the immediate hospitalization. Intensive care and surveillance are necessary. The adequate therapy of ventilatory and circulatory functions is of great importance and should be started by the first consulted physician before transport to the hospital.", "contents": "[Emergency situations in drug addicts. Classification, diagnostic and therapeutic measures]. In drug addicts emergency situations can occur which differ only slightly in symptoms but require different therapeutic measures, depending on type of the drug and symptomatology. Diagnostic and therapeutical difficulties in emergency situations, especially the not unfrequent late complications and the considerable danger of suicidal actions require in general the immediate hospitalization. Intensive care and surveillance are necessary. The adequate therapy of ventilatory and circulatory functions is of great importance and should be started by the first consulted physician before transport to the hospital.", "PMID": 964881}
{"id": "pubmed23n0032_6055", "title": "[Treatment of primary chronic polyarthritis in special cases].", "content": "Empirical medicine has three main supporting pillars: physical treatment, dietetics and phytotherapy. In this area of medicine, a number of therapeutical methods have been evolved which stand up to investigations employing scientific criteria, and which, in consequence, have become officially recognized by medical science. Examples are: diet rich in bulkage, digitalis, products of Papaver somniferum (Poppy) and salicylates. The acceptance of phytotherapeutic agents into the drug armamentarium of scientific medicine presupposes the availability of test methods suitable to demonstrate their effectiveness. Quite a number of remedies that have always been firmly anchored in empirical medicine, but which was applied regularly only by the nature healer, have suddenly, in the light of new test methods, been shown to be effective medicines; thus, for example, onions and garlic are good for thrombotic processes and hyperglycaemia, carminatives for sphincter spasms in the gastrointestinal tract, and alcohol is an appetite-stimulating stomachic. This fact, the limited applicability of the information obtained from animal experiments, and the further fact that even test results obtained in human subjects cannot be applied on a world-wide basis, exhort us to take care not to subscribe to an all-too apodictic classification of therapeutic measures into effective and noneffective. A further point for consideration is that the administration even of what is only a supposed remedy, can trigger or promote healing simply on the strength of the action of the physician. Scientific thinking is an indispensible precondition of the physician's activity--to consider it as the beginning and end of all things medical, however, is simply to demonstrate a narrowness of outlook.", "contents": "[Treatment of primary chronic polyarthritis in special cases]. Empirical medicine has three main supporting pillars: physical treatment, dietetics and phytotherapy. In this area of medicine, a number of therapeutical methods have been evolved which stand up to investigations employing scientific criteria, and which, in consequence, have become officially recognized by medical science. Examples are: diet rich in bulkage, digitalis, products of Papaver somniferum (Poppy) and salicylates. The acceptance of phytotherapeutic agents into the drug armamentarium of scientific medicine presupposes the availability of test methods suitable to demonstrate their effectiveness. Quite a number of remedies that have always been firmly anchored in empirical medicine, but which was applied regularly only by the nature healer, have suddenly, in the light of new test methods, been shown to be effective medicines; thus, for example, onions and garlic are good for thrombotic processes and hyperglycaemia, carminatives for sphincter spasms in the gastrointestinal tract, and alcohol is an appetite-stimulating stomachic. This fact, the limited applicability of the information obtained from animal experiments, and the further fact that even test results obtained in human subjects cannot be applied on a world-wide basis, exhort us to take care not to subscribe to an all-too apodictic classification of therapeutic measures into effective and noneffective. A further point for consideration is that the administration even of what is only a supposed remedy, can trigger or promote healing simply on the strength of the action of the physician. Scientific thinking is an indispensible precondition of the physician's activity--to consider it as the beginning and end of all things medical, however, is simply to demonstrate a narrowness of outlook.", "PMID": 964883}
{"id": "pubmed23n0032_6056", "title": "[Mineralocorticoid syndromes and hypertension].", "content": "Mineralocorticoids are out of the causes of secondary hypertension. Excess production of mineralocorticoids induces sodium and fluid retention, loss of potassium and metabolic alcalosis. The diagnosis of mineralocorticoid syndromes depends on the interpretation of the functional status of the renin-mineralocorticoid-system, which is in part responsible for the maintenance of normal blood pressure. The classical representative of this group is the syndrome of primary aldosteronism. Causes of mineralocorticoid syndromes associated with hypertension are: 1. autonomous production of mineralo-corticoids by an adrenal adenoma or by idiopathic bilateral adrenal hyperplasia; 2. deficiency of adrenal 17-alpha-hydroxylase or of 11-beta-hydroxylase; 3. secondary aldosteronism associated with primary reninism, or renal arterial stenosis; and 4. pseudo aldosteronism due to excessive ingestion of licorice. Malign or essential hypertension may also often be followed by secondary aldosteronism.", "contents": "[Mineralocorticoid syndromes and hypertension]. Mineralocorticoids are out of the causes of secondary hypertension. Excess production of mineralocorticoids induces sodium and fluid retention, loss of potassium and metabolic alcalosis. The diagnosis of mineralocorticoid syndromes depends on the interpretation of the functional status of the renin-mineralocorticoid-system, which is in part responsible for the maintenance of normal blood pressure. The classical representative of this group is the syndrome of primary aldosteronism. Causes of mineralocorticoid syndromes associated with hypertension are: 1. autonomous production of mineralo-corticoids by an adrenal adenoma or by idiopathic bilateral adrenal hyperplasia; 2. deficiency of adrenal 17-alpha-hydroxylase or of 11-beta-hydroxylase; 3. secondary aldosteronism associated with primary reninism, or renal arterial stenosis; and 4. pseudo aldosteronism due to excessive ingestion of licorice. Malign or essential hypertension may also often be followed by secondary aldosteronism.", "PMID": 964885}
{"id": "pubmed23n0032_6057", "title": "[Cerebral embolism as a complication. Incidence and pathogenesis after heart infarct].", "content": "By means of clinical studies the frequency of cerebral embolic incidents after myocardial infarction was clarified. It is referred to the importance of some risk factors. The value of long time anticoagulant therapy is discussed.", "contents": "[Cerebral embolism as a complication. Incidence and pathogenesis after heart infarct]. By means of clinical studies the frequency of cerebral embolic incidents after myocardial infarction was clarified. It is referred to the importance of some risk factors. The value of long time anticoagulant therapy is discussed.", "PMID": 964890}
{"id": "pubmed23n0032_6058", "title": "[Diabetes mellitus and microcirculation. Significance of diabetic angiopathy for the prognosis and course of diabetes mellitus, possibilities and bases of angiologic therapy].", "content": "The importance of diabetic angiopathy for prognosis and course of diabetes mellitus, possibilities and basis of angiological therapy Complications originating from the vascular system determine life expectancy of the diabetic patient. He is particularly endangered by apoplexy, heart attack, arteriosclerosis of the lower extremities, retino- and nephropathy. Microangiopathy is a specific diabetic problem, the development of which shows a clear dependency on the quality of metabolism. Conventional therapy of circulatory problems today is less concerned with the vascular system than with the qualities of blood viscosity. In this context, viscosity is of main concern. Particularly in microcirculation viscosity is dependent on blood factors such as: haematocrit, plasmaviscosity, erythrocytes and thrombocytes. Their changed behaviour results, in the case of diabetes mellitus, in an increase in viscosity partly dependent on metabolism. A promising concept of treatment is available by pharmaceutically influencing the alteration of erythrocytes.", "contents": "[Diabetes mellitus and microcirculation. Significance of diabetic angiopathy for the prognosis and course of diabetes mellitus, possibilities and bases of angiologic therapy]. The importance of diabetic angiopathy for prognosis and course of diabetes mellitus, possibilities and basis of angiological therapy Complications originating from the vascular system determine life expectancy of the diabetic patient. He is particularly endangered by apoplexy, heart attack, arteriosclerosis of the lower extremities, retino- and nephropathy. Microangiopathy is a specific diabetic problem, the development of which shows a clear dependency on the quality of metabolism. Conventional therapy of circulatory problems today is less concerned with the vascular system than with the qualities of blood viscosity. In this context, viscosity is of main concern. Particularly in microcirculation viscosity is dependent on blood factors such as: haematocrit, plasmaviscosity, erythrocytes and thrombocytes. Their changed behaviour results, in the case of diabetes mellitus, in an increase in viscosity partly dependent on metabolism. A promising concept of treatment is available by pharmaceutically influencing the alteration of erythrocytes.", "PMID": 964891}
{"id": "pubmed23n0032_6059", "title": "[Suicide and the weather. Risk of suicide in mental disorders and possibilities for prevention].", "content": "The risk of suicid in psychic disorders and possibilities of suicid prophylaxis The mysterious connection between weather and suicidal acts has been of particular interest at all times. In the past years extensive studies were carried out. But often the results were differing from each other. Nowadays, every person, committing suicid is regarded as psychically disturbed. Therefore, it may be better to analyse the diagnoses retrospectively and to determine which type of psychiatric illness is reacting significantly to which meteorological event. The results of this study using the \"coincidence method\" (n = 17 268) make it possible to give exact clues, which meteorological event may be dangerous to which kind of psychiatric illness. The time before a change of weather is of importance. The daily medical-meteorological telephone-service in the Federal Republic of Germany (bioprog) is therefore a biometeorological contribution to the prophylaxis of suicides.", "contents": "[Suicide and the weather. Risk of suicide in mental disorders and possibilities for prevention]. The risk of suicid in psychic disorders and possibilities of suicid prophylaxis The mysterious connection between weather and suicidal acts has been of particular interest at all times. In the past years extensive studies were carried out. But often the results were differing from each other. Nowadays, every person, committing suicid is regarded as psychically disturbed. Therefore, it may be better to analyse the diagnoses retrospectively and to determine which type of psychiatric illness is reacting significantly to which meteorological event. The results of this study using the \"coincidence method\" (n = 17 268) make it possible to give exact clues, which meteorological event may be dangerous to which kind of psychiatric illness. The time before a change of weather is of importance. The daily medical-meteorological telephone-service in the Federal Republic of Germany (bioprog) is therefore a biometeorological contribution to the prophylaxis of suicides.", "PMID": 964893}
{"id": "pubmed23n0032_6060", "title": "[New method of thrombocyte counting. Technic of electronic counting of low thrombocyte concentrations in peripheral blood].", "content": "Starting from recent studies of Balleisen (1972) and Schmidt et al. (1973) about automated counting of platelets an automated method has been developed for accurate platelet counting at very low platelet concentration of peripheral blood. The method can be summarized as follows: 0,1 ml anticoagulated venous blood (Na-EDTA or polystyrol tubes containing EDTA powder) or capillary blood from finger tips was diluted with 20 ml of a mixture of Isoton-Periston-(Bayer-)Na2-EDTA (22:2:1 vol.) in polystyrol tubes. After 5 min centrifugation at 85 g, using an angle head and decantation into a polystyrol tube, second centrifugation. Decantation in a Coulter glass and counting at aperture 8, attenuation 0.500 and theshold 6 respectively 70 using at 70 mikron capillary. Counts at threshold 70 (erythrocyte counts) were subtracted from counts at threshold 6 (platelets and erythrocytes); to calculate platelet counts/mm3 multiplication by factor 2 at undiluted venous or capillary blood. The mean variation coefficient of our method being 4,1%, counting of platelets is possible with the accuracy of other automated methods at normal platelet concentration. At very low platelet counts our method seems to be the most accurate laboratory method for platelet counting.", "contents": "[New method of thrombocyte counting. Technic of electronic counting of low thrombocyte concentrations in peripheral blood]. Starting from recent studies of Balleisen (1972) and Schmidt et al. (1973) about automated counting of platelets an automated method has been developed for accurate platelet counting at very low platelet concentration of peripheral blood. The method can be summarized as follows: 0,1 ml anticoagulated venous blood (Na-EDTA or polystyrol tubes containing EDTA powder) or capillary blood from finger tips was diluted with 20 ml of a mixture of Isoton-Periston-(Bayer-)Na2-EDTA (22:2:1 vol.) in polystyrol tubes. After 5 min centrifugation at 85 g, using an angle head and decantation into a polystyrol tube, second centrifugation. Decantation in a Coulter glass and counting at aperture 8, attenuation 0.500 and theshold 6 respectively 70 using at 70 mikron capillary. Counts at threshold 70 (erythrocyte counts) were subtracted from counts at threshold 6 (platelets and erythrocytes); to calculate platelet counts/mm3 multiplication by factor 2 at undiluted venous or capillary blood. The mean variation coefficient of our method being 4,1%, counting of platelets is possible with the accuracy of other automated methods at normal platelet concentration. At very low platelet counts our method seems to be the most accurate laboratory method for platelet counting.", "PMID": 964894}
{"id": "pubmed23n0032_6061", "title": "[Artificial anus. Advantages and disadvantages of ileo- and colostomy].", "content": "The majority of patients with ileostomy adapt to their new situation and are able to live a nearly normal professional and social life. This does not mean that there is not a need for improvement in the ileostomy construction. It merely proves the great adaptive mechanism in man. In spite of the improvement in surgical techniques in the construction of the ileostomy and the development of modern ileostomy appliances, a proportion of the patients still experiences serious problems. In order to improve the situation for patients with ileostomy a new type of ileostomy has been developed. From the terminal ileum an intraabdominal, intestinal reservoir is constructed and the outlet from the reservoir is provided with a \"nipple valve\" preventing leakage of gas and faeces through the outlet. The continent ileostomy has now been under clinical trial for more than seven years. The success-rate has increased along with improvements in technique and introduction of methodological modifications. More than 90% of 164 patients provided with this type of ileostomy had at follow-up satisfactory functional results of their ileostomy. That means that they had no need for carrying external ileostomy appliances. A method for constructing a continent colostomy has been tested in dogs. The sigmoid colon was divided and the distal end closed. At the proximal end a \"nipple valve\" was constructed by intussuscepting a part of the intestine into its lumen. All dogs were continent from the time of operation until they were sacrificed one to eight weeks later. The method is now under elaboration for clinical trial.", "contents": "[Artificial anus. Advantages and disadvantages of ileo- and colostomy]. The majority of patients with ileostomy adapt to their new situation and are able to live a nearly normal professional and social life. This does not mean that there is not a need for improvement in the ileostomy construction. It merely proves the great adaptive mechanism in man. In spite of the improvement in surgical techniques in the construction of the ileostomy and the development of modern ileostomy appliances, a proportion of the patients still experiences serious problems. In order to improve the situation for patients with ileostomy a new type of ileostomy has been developed. From the terminal ileum an intraabdominal, intestinal reservoir is constructed and the outlet from the reservoir is provided with a \"nipple valve\" preventing leakage of gas and faeces through the outlet. The continent ileostomy has now been under clinical trial for more than seven years. The success-rate has increased along with improvements in technique and introduction of methodological modifications. More than 90% of 164 patients provided with this type of ileostomy had at follow-up satisfactory functional results of their ileostomy. That means that they had no need for carrying external ileostomy appliances. A method for constructing a continent colostomy has been tested in dogs. The sigmoid colon was divided and the distal end closed. At the proximal end a \"nipple valve\" was constructed by intussuscepting a part of the intestine into its lumen. All dogs were continent from the time of operation until they were sacrificed one to eight weeks later. The method is now under elaboration for clinical trial.", "PMID": 964896}
{"id": "pubmed23n0032_6062", "title": "[Manifestations of systemic diseases in the gastrointestinal tract].", "content": "Under the aspect of systemic diseases and their manifestation in the gut the following conclusions can be drawn: 1. The skin is the mirror of the intestinal tract; not only in primary gastroenterological disorders one should look for dermatological complications, but should also think in chronic skin lesions of concomitant intestinal alterations. 2. In all patients with collagen diseases a gastrointestinal involvement is very common. 3. In all endocrine disorders except in hypothyroidism diarrhea is a very common finding. 4. Infiltrations of gastrointestinal tract can be demonstrated in many cases by gastric, small bowel or rectal biopsy. 5. In all forms of dysgammaglobulinemia giardiasis is very common. 6. In right heart failure protein-losing enteropathy should be considered, in left ventricular insufficiency bowel ischemia.", "contents": "[Manifestations of systemic diseases in the gastrointestinal tract]. Under the aspect of systemic diseases and their manifestation in the gut the following conclusions can be drawn: 1. The skin is the mirror of the intestinal tract; not only in primary gastroenterological disorders one should look for dermatological complications, but should also think in chronic skin lesions of concomitant intestinal alterations. 2. In all patients with collagen diseases a gastrointestinal involvement is very common. 3. In all endocrine disorders except in hypothyroidism diarrhea is a very common finding. 4. Infiltrations of gastrointestinal tract can be demonstrated in many cases by gastric, small bowel or rectal biopsy. 5. In all forms of dysgammaglobulinemia giardiasis is very common. 6. In right heart failure protein-losing enteropathy should be considered, in left ventricular insufficiency bowel ischemia.", "PMID": 964897}
{"id": "pubmed23n0032_6063", "title": "[Heart pacemaker with lithium batteries. 2 years' clinical experience].", "content": "102 lithium-powered cardiac pacemakers manufactured by Cardiac Pacemakers Inc. (CPI 301 UD, 101 UA and 501 UD) were implanted in 94 patients. During the observation time (25 months) the following complications were registered: 2 pulse generator failures, 4 failures of the demand-function, anodic muscle-twitching in 2 patients, 2 erosions of the pacemaker-pocket and 1 hematoma of the pocket. 5 pulse generators were exchanged without malfunction of the pacemaker. In 4 patients reinterventions on the electrode had, to be performed. 4 deaths occurred, none of them related to malfunction of the pacemaker. 2 lithium-powered pacemakers were explanted in other hospitals, for reasons not known to us and 1 patient left the German Federal Republic. 83 patients are under control with well working lithium-powered pacemakers.", "contents": "[Heart pacemaker with lithium batteries. 2 years' clinical experience]. 102 lithium-powered cardiac pacemakers manufactured by Cardiac Pacemakers Inc. (CPI 301 UD, 101 UA and 501 UD) were implanted in 94 patients. During the observation time (25 months) the following complications were registered: 2 pulse generator failures, 4 failures of the demand-function, anodic muscle-twitching in 2 patients, 2 erosions of the pacemaker-pocket and 1 hematoma of the pocket. 5 pulse generators were exchanged without malfunction of the pacemaker. In 4 patients reinterventions on the electrode had, to be performed. 4 deaths occurred, none of them related to malfunction of the pacemaker. 2 lithium-powered pacemakers were explanted in other hospitals, for reasons not known to us and 1 patient left the German Federal Republic. 83 patients are under control with well working lithium-powered pacemakers.", "PMID": 964900}
{"id": "pubmed23n0032_6064", "title": "[Glaucoma prevention].", "content": "All glaucomatous eye damages are caused by a too high intra-ocular pressure. This damage of the eye nerve leads in chronical cases from a slowly increasing loss of the visual field, which is usually not noticed by the patient, to total blindness. Prophylactic examinations should be introduced to all persons over 40 years old. The examination should consist of the measurement of the intra-ocular pressure and should be carried out within a period of 3-5 years.", "contents": "[Glaucoma prevention]. All glaucomatous eye damages are caused by a too high intra-ocular pressure. This damage of the eye nerve leads in chronical cases from a slowly increasing loss of the visual field, which is usually not noticed by the patient, to total blindness. Prophylactic examinations should be introduced to all persons over 40 years old. The examination should consist of the measurement of the intra-ocular pressure and should be carried out within a period of 3-5 years.", "PMID": 964904}
{"id": "pubmed23n0032_6065", "title": "[Contact lenses for children. Indications and results].", "content": "If it is necessary to prescribe contact lenses for children, this correction should be made as soon as possible. As a consequence of the development of soft (hydrophile) lenses, the above mentioned group of patients has increased considerably. In any case, a monolateral aphakia as well as a manifest anisometropia should be corrected by a contact lense to avoid amblyopia, heterotropia and loss of stereoscopic vision. Usually soft lenses are well tolerated, so that patients suffering from aphakia as well as hyperopia should always use this type of lense. Nevertheless, the hard lense still has its indication in treating myopia and high astigmatism.", "contents": "[Contact lenses for children. Indications and results]. If it is necessary to prescribe contact lenses for children, this correction should be made as soon as possible. As a consequence of the development of soft (hydrophile) lenses, the above mentioned group of patients has increased considerably. In any case, a monolateral aphakia as well as a manifest anisometropia should be corrected by a contact lense to avoid amblyopia, heterotropia and loss of stereoscopic vision. Usually soft lenses are well tolerated, so that patients suffering from aphakia as well as hyperopia should always use this type of lense. Nevertheless, the hard lense still has its indication in treating myopia and high astigmatism.", "PMID": 964905}
{"id": "pubmed23n0032_6066", "title": "[Value of the results of animal experiments for the evaluation of drugs. 2. Methodology and prediction of effects].", "content": "The efficiency of animal trials is critically examined against the background of the new EC drug regulations. The present contributions deal with the problem of predictability of qualitative events. It is established that the so-called \"pharmacological properties\" of substances do not exist. Further it must be taken into account that observations made on test animals are reductionistic in relation to man. The study also shows that it is difficult to predict events with reasonable certainty even within one particular animal species. The problem of extrapolation is being viewed under three different aspects: when there is no previous experience at all, when there is relevant previous experience, and when there are animal models available. In the first case, it is impossible to make any prediction at all. In the second case, only subjective suppositions are possible. As to the third case, it is an open question at the moment whether animal models do exist at all. According to the present state of science it is not possible to make objective predictions for man on the basis of animal trials. In regard to animal trials, the EC regulations stand in contrast to science and the methodology of statistics. The subjective nature of prediction will be discussed in the following contributions.", "contents": "[Value of the results of animal experiments for the evaluation of drugs. 2. Methodology and prediction of effects]. The efficiency of animal trials is critically examined against the background of the new EC drug regulations. The present contributions deal with the problem of predictability of qualitative events. It is established that the so-called \"pharmacological properties\" of substances do not exist. Further it must be taken into account that observations made on test animals are reductionistic in relation to man. The study also shows that it is difficult to predict events with reasonable certainty even within one particular animal species. The problem of extrapolation is being viewed under three different aspects: when there is no previous experience at all, when there is relevant previous experience, and when there are animal models available. In the first case, it is impossible to make any prediction at all. In the second case, only subjective suppositions are possible. As to the third case, it is an open question at the moment whether animal models do exist at all. According to the present state of science it is not possible to make objective predictions for man on the basis of animal trials. In regard to animal trials, the EC regulations stand in contrast to science and the methodology of statistics. The subjective nature of prediction will be discussed in the following contributions.", "PMID": 964908}
{"id": "pubmed23n0032_6067", "title": "[Malignant systemic diseases of the digestive tract].", "content": "In about 5-6% of all patients with malignant myeloproliferative disorders the gastrointestinal tract is involved with macroscopic lesions. The most common sites of digestive manifestations are stomach, small bowel, pancreas and colon. Multiple irregular ulcers, doughy infiltration of the bowel wall, multiple nodules and diffuse polyposis of the colon are classic patterns of secondary lymphoma of the gut. Prognosis is considerably impaired by gastroenterological symptoms.", "contents": "[Malignant systemic diseases of the digestive tract]. In about 5-6% of all patients with malignant myeloproliferative disorders the gastrointestinal tract is involved with macroscopic lesions. The most common sites of digestive manifestations are stomach, small bowel, pancreas and colon. Multiple irregular ulcers, doughy infiltration of the bowel wall, multiple nodules and diffuse polyposis of the colon are classic patterns of secondary lymphoma of the gut. Prognosis is considerably impaired by gastroenterological symptoms.", "PMID": 964909}
{"id": "pubmed23n0032_6068", "title": "[Comparative studies of the psychosomatic aspects of liver diseases].", "content": "Connections between disturbed functions of liver and mental diseases at all times have been generally supposed as existent and have been discussed in the literature; they should be clarified by means of questionnaire-tests: MPI-test and MMQ-test of Eysenck combined with neurotizism-depression-questionnaire of Hoffmann-La Roche allowed to comprehend the dimensions of personality \"neurotizism\" and \"depressivity\". Concerning \"neurotizism\" statistically significant differences could be found between liver-patients and healthy persons in a tendency to a significant increase among liver-patients, but not between liver-patients with other diseases. The tendency to \"depressivity\" of healthy persons could be statistically evaluated as being much greater than that of liver-patients; there were no differences between liver-patients and patients with other diseases. That is why liver-patients do not differ psychically from patients suffering from other diseases; they share their reaction to the occurrence of disease - in various degrees until a neurotic lack of active realization of the fact of illness. Liver-diseases apparently are not triggered or caused by special pre-morbid alterations.", "contents": "[Comparative studies of the psychosomatic aspects of liver diseases]. Connections between disturbed functions of liver and mental diseases at all times have been generally supposed as existent and have been discussed in the literature; they should be clarified by means of questionnaire-tests: MPI-test and MMQ-test of Eysenck combined with neurotizism-depression-questionnaire of Hoffmann-La Roche allowed to comprehend the dimensions of personality \"neurotizism\" and \"depressivity\". Concerning \"neurotizism\" statistically significant differences could be found between liver-patients and healthy persons in a tendency to a significant increase among liver-patients, but not between liver-patients with other diseases. The tendency to \"depressivity\" of healthy persons could be statistically evaluated as being much greater than that of liver-patients; there were no differences between liver-patients and patients with other diseases. That is why liver-patients do not differ psychically from patients suffering from other diseases; they share their reaction to the occurrence of disease - in various degrees until a neurotic lack of active realization of the fact of illness. Liver-diseases apparently are not triggered or caused by special pre-morbid alterations.", "PMID": 964910}
{"id": "pubmed23n0032_6069", "title": "[Desire for children and the practice of family planning].", "content": "Of 1647 women in confinement (mean age 26years) and 185 students nurses (mean age 18 years), the majority wanted two children. 8% of the women in confinement and 2% of the student nurses had received no previous family planning information, whereas 38% of the women in confinement and 81% of the student nurses had practiced contraceptive methods with a Pearl-index of 3 or less (oral contraceptives, basal-temperature-method). 31% of the women in confinement had already had unplanned pregnancies (mean: 1.4 pregnancy per woman). The last pregnancy was not planned in 27% and in these 4 out of 5 pregnancies were caused by incorrect application of the contraceptive method. Faulty practices and unsatisfactory results, in spite of the availability of secure contraceptive methods, emphasize the necessity of intensifying family planning. Because programmes including personal counsel have yielded better results, these should be favoured and include a clinical post partum programme. The latter was considered necessary by 95% of the women attending such a programme in our hospital.", "contents": "[Desire for children and the practice of family planning]. Of 1647 women in confinement (mean age 26years) and 185 students nurses (mean age 18 years), the majority wanted two children. 8% of the women in confinement and 2% of the student nurses had received no previous family planning information, whereas 38% of the women in confinement and 81% of the student nurses had practiced contraceptive methods with a Pearl-index of 3 or less (oral contraceptives, basal-temperature-method). 31% of the women in confinement had already had unplanned pregnancies (mean: 1.4 pregnancy per woman). The last pregnancy was not planned in 27% and in these 4 out of 5 pregnancies were caused by incorrect application of the contraceptive method. Faulty practices and unsatisfactory results, in spite of the availability of secure contraceptive methods, emphasize the necessity of intensifying family planning. Because programmes including personal counsel have yielded better results, these should be favoured and include a clinical post partum programme. The latter was considered necessary by 95% of the women attending such a programme in our hospital.", "PMID": 964912}
{"id": "pubmed23n0032_6070", "title": "[Ambulatory tubal sterilization using a plastic clip].", "content": "The ambulant sterilization of the ovarien tubes with the plastic clip represents a notable method among the operative sterilization methods. With this method, failures can be totally avoided. The bloodless proceeding in the intraperitoneal region results in a minimum of operational risks. The postoperative morbidity is 0%. The clip sterilization takes a few minutes and can be done with intubation or Trapanal/fluothane narcosis in the ambulance. The possibility of refertilization is still to be discussed. From April 1974 till April 1975 this method for sterilization was employed in 97 patients. There were no complications or failures seen.", "contents": "[Ambulatory tubal sterilization using a plastic clip]. The ambulant sterilization of the ovarien tubes with the plastic clip represents a notable method among the operative sterilization methods. With this method, failures can be totally avoided. The bloodless proceeding in the intraperitoneal region results in a minimum of operational risks. The postoperative morbidity is 0%. The clip sterilization takes a few minutes and can be done with intubation or Trapanal/fluothane narcosis in the ambulance. The possibility of refertilization is still to be discussed. From April 1974 till April 1975 this method for sterilization was employed in 97 patients. There were no complications or failures seen.", "PMID": 964913}
{"id": "pubmed23n0032_6071", "title": "[Copper-7 intrauterine device (Gravigard). Report of experience].", "content": "Within 2 years 258 women aged 16 to 42 years were fitted with a copper-7-IUD (Gravigard, Searle). 45% of these patients were nulliparous women. Follow-up studies were provided in 236 cases in a total of 2717 women-months according to an average time of observation of 11.5 women-months per patient. Altogether there occurred 5 unwanted pregnancies with a Pearl-Index of 2.3. Partial or total expulsion occurred in 27 cases (11%). Fourteen out of 27 expulsions were noticed within the first 2 months after insertion. Removals for medical reasons were requested in 36 cases-in the majority (26 cases) because of bleeding and pain. Thirteen women desired removal of the IUD in order to get either pregnant or sterilized. The continuation rate after 2 years was 73%. The highest number of drop-outs occurred within the first year after insertion (60 cases = 23%) whereas only 4% drop-outs occurred in the 2nd year. It was suggested that the low continuation rate might be partially due to the high number of physicians (19) participating in this study. The most important side effects of the copper-7-IUD in situ were irregular bleeding, intermenstrual pain and discharge. Irregular bleeding and pain decreased markedly after the first year, while slightly prolonged periods persisted in 30 to 50% of all patients. Compared with oral contraceptives copper IUDs hav less severe side effects but less safety at the same time-especially for the younger fertile age group. Therefore they should be offered to young nulliparous women only for a limited period of time.", "contents": "[Copper-7 intrauterine device (Gravigard). Report of experience]. Within 2 years 258 women aged 16 to 42 years were fitted with a copper-7-IUD (Gravigard, Searle). 45% of these patients were nulliparous women. Follow-up studies were provided in 236 cases in a total of 2717 women-months according to an average time of observation of 11.5 women-months per patient. Altogether there occurred 5 unwanted pregnancies with a Pearl-Index of 2.3. Partial or total expulsion occurred in 27 cases (11%). Fourteen out of 27 expulsions were noticed within the first 2 months after insertion. Removals for medical reasons were requested in 36 cases-in the majority (26 cases) because of bleeding and pain. Thirteen women desired removal of the IUD in order to get either pregnant or sterilized. The continuation rate after 2 years was 73%. The highest number of drop-outs occurred within the first year after insertion (60 cases = 23%) whereas only 4% drop-outs occurred in the 2nd year. It was suggested that the low continuation rate might be partially due to the high number of physicians (19) participating in this study. The most important side effects of the copper-7-IUD in situ were irregular bleeding, intermenstrual pain and discharge. Irregular bleeding and pain decreased markedly after the first year, while slightly prolonged periods persisted in 30 to 50% of all patients. Compared with oral contraceptives copper IUDs hav less severe side effects but less safety at the same time-especially for the younger fertile age group. Therefore they should be offered to young nulliparous women only for a limited period of time.", "PMID": 964914}
{"id": "pubmed23n0032_6072", "title": "Demonstration of neuraminidase activity in human blood serum and human milk using a modified, radioactively labelled alpha1-glycoprotein as substrate.", "content": "N-Acetylneuramini acid of alpha1-glycoprotein was oxidized with a small molar excess of periodate and reduced with tritium-labelled borohydride. By this method about 50% of the N-acetylneuraminic acid was converted to its radioactively labelled C8-analog and 25% to its C7-analog. Using this modified alpha1-glycoprotein as substrate, minimum neuraminidase concentrations of 10(-18) units/ml, related to the activity of neuraminidase from Vibrio cholerae, could be determined. Neuraminidase activity was demonstrated in 95% of the sera or blood plasma samples from a series of 417 healthy or ill human individuals and in milk samples from 5 different donors. The neuraminidase in both serum and milk had optimal activity at pH 5.5. On an average, 10(-10) neuraminidase units were found in 1 ml serum and 10(-8) units in 1 ml milk. Although the neuraminidase activities in the sera varied, a correlation with definite pathological states is not yet possible. N-Acetylneuraminate pyruvate-lyase activity could not be detected in human serum.", "contents": "Demonstration of neuraminidase activity in human blood serum and human milk using a modified, radioactively labelled alpha1-glycoprotein as substrate. N-Acetylneuramini acid of alpha1-glycoprotein was oxidized with a small molar excess of periodate and reduced with tritium-labelled borohydride. By this method about 50% of the N-acetylneuraminic acid was converted to its radioactively labelled C8-analog and 25% to its C7-analog. Using this modified alpha1-glycoprotein as substrate, minimum neuraminidase concentrations of 10(-18) units/ml, related to the activity of neuraminidase from Vibrio cholerae, could be determined. Neuraminidase activity was demonstrated in 95% of the sera or blood plasma samples from a series of 417 healthy or ill human individuals and in milk samples from 5 different donors. The neuraminidase in both serum and milk had optimal activity at pH 5.5. On an average, 10(-10) neuraminidase units were found in 1 ml serum and 10(-8) units in 1 ml milk. Although the neuraminidase activities in the sera varied, a correlation with definite pathological states is not yet possible. N-Acetylneuraminate pyruvate-lyase activity could not be detected in human serum.", "PMID": 964915}
{"id": "pubmed23n0032_6073", "title": "Metabolic transformation of colchicine, IV[1]. On the interaction of colchicine and colchiceine with sulfhydryl compounds.", "content": "The interaction of colchicine and one of its metabolites, O10-demethylcolchicine (colchiceine), with sulfhydryl compounds was studied in several experimental systems. Colchiceine protects reduced glutathione from oxidation by atmospheric oxygen. Inactivation of enzymes with catalytically essential sulfhydryl groups is prevented by colchiceine. In affinity chromatographic experiments, Agarose-bound colchiceine adsorbs sulfhydryl enzymes which may be eluted specifically by SH-compounds like mercaptoethanol. No such effects are shown by colchicine. The data demonstrate a novel type of biochemical reactivity in the metabolite colchiceine with potential biological relevance.", "contents": "Metabolic transformation of colchicine, IV[1]. On the interaction of colchicine and colchiceine with sulfhydryl compounds. The interaction of colchicine and one of its metabolites, O10-demethylcolchicine (colchiceine), with sulfhydryl compounds was studied in several experimental systems. Colchiceine protects reduced glutathione from oxidation by atmospheric oxygen. Inactivation of enzymes with catalytically essential sulfhydryl groups is prevented by colchiceine. In affinity chromatographic experiments, Agarose-bound colchiceine adsorbs sulfhydryl enzymes which may be eluted specifically by SH-compounds like mercaptoethanol. No such effects are shown by colchicine. The data demonstrate a novel type of biochemical reactivity in the metabolite colchiceine with potential biological relevance.", "PMID": 964916}
{"id": "pubmed23n0032_6074", "title": "Uptake of cortisol by isolated rat liver cells. A phenomenon indicative of carrier-mediation and simple diffusion.", "content": "The uptake of cortisol by isolated rat liver cells was studied. Cortisol was taken up rapidly; the uptake increased with increasing temperature and reached a plateau after 45 s at 37 degrees, C, after 60 s at 27 degrees C, and after 90 s at 22 degrees C; at 5 degrees C the uptake increased linearly with time. The uptake was linear up to 1.5 mg of cell protein. Analysis of uptake as a function of increasing concentration of cortisol in the external medium indicated the presence of two saturable systems: a high-affinity system with an apparent Km value of 190 +/- 25 nM and a low-affinity system with an apparent Km value of 2200 +/- 180 nM. Above 600 nM, the rate of uptake of cortisol increased almost linearly with increasing cortisol concentration. Treatment of cells with KCN or 2,4-dinitrophenol inhibited the two saturable components, leaving the nonsaturable system unaffected. The affinity constants, Ka, were 6 X 10(6) M-1 and 0.6 X 10(6) M-1 for the high and low affinity components, respectively. These values increased approximately two-fold when uptake rates were corrected for diffusion. Cortisone and corticosterone inhibited the uptake of cortisol by liver cells competitively; dexamethasone inhibited cortisol uptake noncompetitively. Similarly, oestrone, oestradiol and testosterone decreased the uptake of cortisol, at a concentration of 2000 nM in the external medium, by 20, 49 and 35 percent, respectively; the inhibition was noncompetitive. p-Chloromercuribenzoate, N-ethylmaleimide and 1-fluoro-2,4-dinitrobenzene decreased the uptake of cortisol. Ouabain did not influence the uptake of cortisol; varying the external sodium concentration also did not affect uptake of cortisol. Cyclic-3',5'-adenosine monophosphate had a stimulatory effect. The results show that the first step, before cortisol is bound to intracellular binding proteins, is the uptake of cortisol by proteins in the plasma membrane. At lower concentrations of cortisol, uptake takes place by saturable processes; at higher concentrations saturation is not achieved, indicating that simple diffusion becomes the major route of transport into the cell. The proteins in the plasma membrane probably function as carriers to transport the glucocorticoid into the cell.", "contents": "Uptake of cortisol by isolated rat liver cells. A phenomenon indicative of carrier-mediation and simple diffusion. The uptake of cortisol by isolated rat liver cells was studied. Cortisol was taken up rapidly; the uptake increased with increasing temperature and reached a plateau after 45 s at 37 degrees, C, after 60 s at 27 degrees C, and after 90 s at 22 degrees C; at 5 degrees C the uptake increased linearly with time. The uptake was linear up to 1.5 mg of cell protein. Analysis of uptake as a function of increasing concentration of cortisol in the external medium indicated the presence of two saturable systems: a high-affinity system with an apparent Km value of 190 +/- 25 nM and a low-affinity system with an apparent Km value of 2200 +/- 180 nM. Above 600 nM, the rate of uptake of cortisol increased almost linearly with increasing cortisol concentration. Treatment of cells with KCN or 2,4-dinitrophenol inhibited the two saturable components, leaving the nonsaturable system unaffected. The affinity constants, Ka, were 6 X 10(6) M-1 and 0.6 X 10(6) M-1 for the high and low affinity components, respectively. These values increased approximately two-fold when uptake rates were corrected for diffusion. Cortisone and corticosterone inhibited the uptake of cortisol by liver cells competitively; dexamethasone inhibited cortisol uptake noncompetitively. Similarly, oestrone, oestradiol and testosterone decreased the uptake of cortisol, at a concentration of 2000 nM in the external medium, by 20, 49 and 35 percent, respectively; the inhibition was noncompetitive. p-Chloromercuribenzoate, N-ethylmaleimide and 1-fluoro-2,4-dinitrobenzene decreased the uptake of cortisol. Ouabain did not influence the uptake of cortisol; varying the external sodium concentration also did not affect uptake of cortisol. Cyclic-3',5'-adenosine monophosphate had a stimulatory effect. The results show that the first step, before cortisol is bound to intracellular binding proteins, is the uptake of cortisol by proteins in the plasma membrane. At lower concentrations of cortisol, uptake takes place by saturable processes; at higher concentrations saturation is not achieved, indicating that simple diffusion becomes the major route of transport into the cell. The proteins in the plasma membrane probably function as carriers to transport the glucocorticoid into the cell.", "PMID": 964917}
{"id": "pubmed23n0032_6075", "title": "The complete amino acid sequences of both subunits of the sweet protein monellin.", "content": "The amino acid sequences of both chains of the sweet protein Monellin have been determined. Since chain separation could not be accomplished easily, cyanogen bromide cleavage at the only methionine residue (in the B-chain) was performed and the three products obtained after cyanogen bromide cleavage were separated. For the identification of amino acid phenylthiohydantoins, high performance liquid chromatography was employed. Thus 37 out of a total of 44 residues of the A chain and 40 out of a total of 42 residues of the large CNBr fragment of the B chain could be determined after Edman degradation of the polypeptides on an automated sequenator.", "contents": "The complete amino acid sequences of both subunits of the sweet protein monellin. The amino acid sequences of both chains of the sweet protein Monellin have been determined. Since chain separation could not be accomplished easily, cyanogen bromide cleavage at the only methionine residue (in the B-chain) was performed and the three products obtained after cyanogen bromide cleavage were separated. For the identification of amino acid phenylthiohydantoins, high performance liquid chromatography was employed. Thus 37 out of a total of 44 residues of the A chain and 40 out of a total of 42 residues of the large CNBr fragment of the B chain could be determined after Edman degradation of the polypeptides on an automated sequenator.", "PMID": 964918}
{"id": "pubmed23n0032_6076", "title": "[Quantitative determination of taurine and glycine in the picomole range using [14C]dansyl chloride (author's transl)].", "content": "Taurine, a possible neurotransmitter, and the transmitter substance glycine can be determined quantitatively in the picomole range using [14C]dansyl chloride even in the presence of a several thousand fold excess of other amino acids. However, it is necessary to eliminate the excess of dansyl chloride after complete reaction by adding diethylamine. In corresponding microchromatograms on 3 X 3 cm polyamide sheets the dansyltaurine is clearly separated as a circumscript spot without fluorescing contaminations. But commercial [14C]dansyl chloride preparations regularly contain radioactive impurities which do not fluoresce and therefore can only be detected by autoradiography. Since one of these labelled impurities migrates with the usual solvents together with [14C]dansyltaurine during chromatography a method is described for a chromatographic separation of [14C]dansyltaurine from these impurities. As internal standards for preparing calibration curves, alpha-phenylglycine and sarcosine are used. gamma-Aminobutyric acid as well as glutamic acid can react with dansyl chloride to form the corresponding lactams. These lactam rings can be converted with ammonia into the corresponding amides (dansylglutamine and dansyl-gamma-aminobutyramide).", "contents": "[Quantitative determination of taurine and glycine in the picomole range using [14C]dansyl chloride (author's transl)]. Taurine, a possible neurotransmitter, and the transmitter substance glycine can be determined quantitatively in the picomole range using [14C]dansyl chloride even in the presence of a several thousand fold excess of other amino acids. However, it is necessary to eliminate the excess of dansyl chloride after complete reaction by adding diethylamine. In corresponding microchromatograms on 3 X 3 cm polyamide sheets the dansyltaurine is clearly separated as a circumscript spot without fluorescing contaminations. But commercial [14C]dansyl chloride preparations regularly contain radioactive impurities which do not fluoresce and therefore can only be detected by autoradiography. Since one of these labelled impurities migrates with the usual solvents together with [14C]dansyltaurine during chromatography a method is described for a chromatographic separation of [14C]dansyltaurine from these impurities. As internal standards for preparing calibration curves, alpha-phenylglycine and sarcosine are used. gamma-Aminobutyric acid as well as glutamic acid can react with dansyl chloride to form the corresponding lactams. These lactam rings can be converted with ammonia into the corresponding amides (dansylglutamine and dansyl-gamma-aminobutyramide).", "PMID": 964919}
{"id": "pubmed23n0032_6077", "title": "Storage of mannose-containing material in cultured human mannosidosis cells and metabolic correction by pig kidney alpha-mannosidase.", "content": "Skin fibroblasts from healthy individuals and a mannosidosis patient were cultured in the presence of [2-3H] mannose and the cell homogenates were fractionated by trichloroacetic acid precipitation into a precipitable and a non-precipitable portion. In uptake as well as in chase experiments the precipitable fractions show no significant difference in their content of radioactivity, while an increased level of radioactivity is found in the non-precipitable fraction of mannosidosis cells. This higher radioactivity content is due to a higher mannose content and is caused by a slower degradation of this fraction. The differences between the metabolisms of the two cell lines can be expressed by the ratio of radioactivity in the non-precipitable and the precipitable fractions. This value is about three times higher for mannosidosis than for control cells. Pig kidney alpha-mannosidase is taken up by both cell lines and is able to correct the impaired degradation of the non-precipitable material in mannosidosis cells, as shown by the normalization of the above defined ratio of radioactivity for this cell type.", "contents": "Storage of mannose-containing material in cultured human mannosidosis cells and metabolic correction by pig kidney alpha-mannosidase. Skin fibroblasts from healthy individuals and a mannosidosis patient were cultured in the presence of [2-3H] mannose and the cell homogenates were fractionated by trichloroacetic acid precipitation into a precipitable and a non-precipitable portion. In uptake as well as in chase experiments the precipitable fractions show no significant difference in their content of radioactivity, while an increased level of radioactivity is found in the non-precipitable fraction of mannosidosis cells. This higher radioactivity content is due to a higher mannose content and is caused by a slower degradation of this fraction. The differences between the metabolisms of the two cell lines can be expressed by the ratio of radioactivity in the non-precipitable and the precipitable fractions. This value is about three times higher for mannosidosis than for control cells. Pig kidney alpha-mannosidase is taken up by both cell lines and is able to correct the impaired degradation of the non-precipitable material in mannosidosis cells, as shown by the normalization of the above defined ratio of radioactivity for this cell type.", "PMID": 964924}
{"id": "pubmed23n0032_6078", "title": "Determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain.", "content": "Reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. The ability of the method to distinguish between arginine and lysine residues was examined. Trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (Kunitz), peanuts and porcine seminal plasma. Inhibitors of the lysine-type were not inactivated by 1,2-cyclohexanedione/borate, e.g. the bovine trypsin/kallikrein inhibitor, the trypsin inhibitors from cow colostrum, porcine pancreas, or snail epidermis (isoinhibitor K from Helix pomatia), and the human alpha1-proteinase inhibitor (alpha1-antitrypsin). However, 2,3-butanedione/borate does not show this high specificity. The method was applied to four high-molecular weight proteinase inhibitors from the albumin gland of the snail (Helix pomatia). All four inhibitors were subject to inactivation by the reagent, indicating an arginine residue at the reactive site. Evidence is given by NMR, UV, and titration data that a complex is formed from 1,2-cyclohexanedione and borate. Due to this complex formation, the amount of free carbonyl component is drastically decreased. Since 1,2-cyclohexanedione without borate lacks the high specificity for arginine residues, the borate complex of the reagent is presumed to be responsible for the high specificity. The new procedure for identification of reactive sites is based on a reversible modification of the guanidium group of the arginine residues.", "contents": "Determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain. Reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. The ability of the method to distinguish between arginine and lysine residues was examined. Trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (Kunitz), peanuts and porcine seminal plasma. Inhibitors of the lysine-type were not inactivated by 1,2-cyclohexanedione/borate, e.g. the bovine trypsin/kallikrein inhibitor, the trypsin inhibitors from cow colostrum, porcine pancreas, or snail epidermis (isoinhibitor K from Helix pomatia), and the human alpha1-proteinase inhibitor (alpha1-antitrypsin). However, 2,3-butanedione/borate does not show this high specificity. The method was applied to four high-molecular weight proteinase inhibitors from the albumin gland of the snail (Helix pomatia). All four inhibitors were subject to inactivation by the reagent, indicating an arginine residue at the reactive site. Evidence is given by NMR, UV, and titration data that a complex is formed from 1,2-cyclohexanedione and borate. Due to this complex formation, the amount of free carbonyl component is drastically decreased. Since 1,2-cyclohexanedione without borate lacks the high specificity for arginine residues, the borate complex of the reagent is presumed to be responsible for the high specificity. The new procedure for identification of reactive sites is based on a reversible modification of the guanidium group of the arginine residues.", "PMID": 964925}
{"id": "pubmed23n0032_6079", "title": "Occurrence of multiple forms of bull and ram acrosin during proenzyme activation and inhibition of activation by p-nitrophenyl p'-guanidinobenzoate.", "content": "Proacrosin was extracted from freshly ejaculated bull and ram spermatozoa with acidic buffer solution pH 3.0, and was partially purified by gel filtration to remove acrosin inhibitors. The occurrence of multiple active forms during proacrosin activation at pH 7.8 was monitored by active enzyme staining of samples after polyacrylamide gel electrophoresis at pH 3.8. For comparison, the protein pattern of such activation samples was also determined after sodium dodecylsulfate-polyacrylamide gel electrophoresis. Proacrosin activation was completely prevented in the presence of 10(-4) M p-nitrophenyl p'-guanidinobenzoate.", "contents": "Occurrence of multiple forms of bull and ram acrosin during proenzyme activation and inhibition of activation by p-nitrophenyl p'-guanidinobenzoate. Proacrosin was extracted from freshly ejaculated bull and ram spermatozoa with acidic buffer solution pH 3.0, and was partially purified by gel filtration to remove acrosin inhibitors. The occurrence of multiple active forms during proacrosin activation at pH 7.8 was monitored by active enzyme staining of samples after polyacrylamide gel electrophoresis at pH 3.8. For comparison, the protein pattern of such activation samples was also determined after sodium dodecylsulfate-polyacrylamide gel electrophoresis. Proacrosin activation was completely prevented in the presence of 10(-4) M p-nitrophenyl p'-guanidinobenzoate.", "PMID": 964926}
{"id": "pubmed23n0032_6080", "title": "[Binding of insulin analogs to partially purified insulin receptor from rat liver membrane (author's transl)].", "content": "The insulin binding fraction was solubilized from rat liver membrane vesicles by triton and partially purified up to the specific binding activity of 2.1 pmol/mg. A further characteristic of the partially purified soluble receptor is a decrease in irreversible binding to 0.36% (+/- 0.28) with regard to total iodine insulin and to (+/- 1.8) in comparison to reversible binding. From Scatchard plots a high affinity binding site (KD = 5 X 10(-10)M) with low capacity (5 pmol/mg) and a low affinity binding site (KD = 3 X 10(-8) M) with high capacity (30 pmol/mg) can be seen. With carefully prepared liver membrane vesicles, the dissociation constant of the high affinity binding site from Scatchard plot is only 2 X 10(-9)M. With liver membrane vesicles, isolated for preparative purification procedure, the high affinity binding site could not be demonstrated. Displacement studies with insulin analogs were performed with [A1-D-alanine] insulin, [A1-L-alanine] insulin, [des-Gly-A1, NB1, NB29-(Msc)2]-insulin, proinsulin and [desoctapeptid B23-B30]-insulin. Results of binding measurements are presented in half-maximal iodo-insulin binding, in determination of inhibitor- and dissociation constants from Dixon-, Scatchard- and Lineweaver-Burk plots. There are equal relative binding potencies of analogs, observed with crude membrane vesicles and partially purified soluble receptor, although there is a 50-fold difference in specific binding activity. Biologically active insulins are characterized by strong binding to the high affinity binding site. The binding to the low affinity binding site is not correlated to the biological activity of the insulin analog. With insulin and biologically responsive analogs a non-linear curve in the double-reciprocal Lineweaver-Burk plot can be observed. Analogs with low biological activity show a linear dependency. Functional interactions of insulin with the receptor can be demonstrated in a high affinity binding with the first binding site of the Scatchard plot and in a non-linear hyperbolic Lineweaver-Burk plot.", "contents": "[Binding of insulin analogs to partially purified insulin receptor from rat liver membrane (author's transl)]. The insulin binding fraction was solubilized from rat liver membrane vesicles by triton and partially purified up to the specific binding activity of 2.1 pmol/mg. A further characteristic of the partially purified soluble receptor is a decrease in irreversible binding to 0.36% (+/- 0.28) with regard to total iodine insulin and to (+/- 1.8) in comparison to reversible binding. From Scatchard plots a high affinity binding site (KD = 5 X 10(-10)M) with low capacity (5 pmol/mg) and a low affinity binding site (KD = 3 X 10(-8) M) with high capacity (30 pmol/mg) can be seen. With carefully prepared liver membrane vesicles, the dissociation constant of the high affinity binding site from Scatchard plot is only 2 X 10(-9)M. With liver membrane vesicles, isolated for preparative purification procedure, the high affinity binding site could not be demonstrated. Displacement studies with insulin analogs were performed with [A1-D-alanine] insulin, [A1-L-alanine] insulin, [des-Gly-A1, NB1, NB29-(Msc)2]-insulin, proinsulin and [desoctapeptid B23-B30]-insulin. Results of binding measurements are presented in half-maximal iodo-insulin binding, in determination of inhibitor- and dissociation constants from Dixon-, Scatchard- and Lineweaver-Burk plots. There are equal relative binding potencies of analogs, observed with crude membrane vesicles and partially purified soluble receptor, although there is a 50-fold difference in specific binding activity. Biologically active insulins are characterized by strong binding to the high affinity binding site. The binding to the low affinity binding site is not correlated to the biological activity of the insulin analog. With insulin and biologically responsive analogs a non-linear curve in the double-reciprocal Lineweaver-Burk plot can be observed. Analogs with low biological activity show a linear dependency. Functional interactions of insulin with the receptor can be demonstrated in a high affinity binding with the first binding site of the Scatchard plot and in a non-linear hyperbolic Lineweaver-Burk plot.", "PMID": 964927}
{"id": "pubmed23n0032_6081", "title": "On the biosynthesis of plasmalogens during myelination in the rat. VIII[1]. Incorporation of 1-[1-14C] alkyl-2-acyl-3-sn-glycerophosphoethanolamine with different fatty acids.", "content": "The uptake of 14C labelled alkyl chains into the alkenyl chains of plasmalogens was estimated 12, 24, 48 and 72 h after intracerebral administration of different substrates to 14 day-old rats  We used as substrates 1-[1-14C] alkyl-3-sn-glycerophosphoethanolamines, containing in the 2-position of the glycerol residue a stearoyl (substrate I), an oleoyl (II), an archidonoyl (III), a 4, 7, 10, 13, 16, 19-docosahexaenoyl (IV) or no acyl residue (substrate V, so-called lysoether phosphatide). 1) If the fatty acid in the 2-position of the substrate is a saturated one (as in experiment I), the recovery of radioactive alkyl chains is relatively high, but their desaturation is very slow and seems not to reach its maximum even after 72 h. 2) If a substrate with an unsaturated fatty acid in the 2-position is applied, the number of radioactive chains (alkyl plus alkenyl) depends on the chain length and/or the number of double bonds in the fatty acids. The desaturation of the alkyl chains, however, reaches its maximum about 48 h after application (except for substrate IV). 3) Not only is the recovery of radioactivity in the glycerophosphoethanolamine lipids by far the highest in experiment V at any time, but also the total activity of the alkenyl chains has already reached a maximum 24 h after application.", "contents": "On the biosynthesis of plasmalogens during myelination in the rat. VIII[1]. Incorporation of 1-[1-14C] alkyl-2-acyl-3-sn-glycerophosphoethanolamine with different fatty acids. The uptake of 14C labelled alkyl chains into the alkenyl chains of plasmalogens was estimated 12, 24, 48 and 72 h after intracerebral administration of different substrates to 14 day-old rats  We used as substrates 1-[1-14C] alkyl-3-sn-glycerophosphoethanolamines, containing in the 2-position of the glycerol residue a stearoyl (substrate I), an oleoyl (II), an archidonoyl (III), a 4, 7, 10, 13, 16, 19-docosahexaenoyl (IV) or no acyl residue (substrate V, so-called lysoether phosphatide). 1) If the fatty acid in the 2-position of the substrate is a saturated one (as in experiment I), the recovery of radioactive alkyl chains is relatively high, but their desaturation is very slow and seems not to reach its maximum even after 72 h. 2) If a substrate with an unsaturated fatty acid in the 2-position is applied, the number of radioactive chains (alkyl plus alkenyl) depends on the chain length and/or the number of double bonds in the fatty acids. The desaturation of the alkyl chains, however, reaches its maximum about 48 h after application (except for substrate IV). 3) Not only is the recovery of radioactivity in the glycerophosphoethanolamine lipids by far the highest in experiment V at any time, but also the total activity of the alkenyl chains has already reached a maximum 24 h after application.", "PMID": 964928}
{"id": "pubmed23n0032_6082", "title": "On the mode of action of eucaryotic initiation factor 1.", "content": "A factor isolated from the human tonsillar ribosomal wash specifically stimulated the poly (U)-dependent binding of Phe-tRNA to 40S subunits at low Mg2+ concentration and without any requirement for GTP. The stimulated binding of Phe-tRNA to 40S particles was inhibited in proportion to added deacylated tRNA. The factor was inactivated by N-ethylmaleimide, but in the presence of 40S subunits a considerable protection was observed. 40S subunits, incubated with the factor and isolated by centrifugation, carried significant factor activity. The results imply that the human tonsillar factor, which shows a great functional analogy to the eucaryotic initiation factor 1 from other sources, exerts its effect by an initial interaction with the 40S subunit.", "contents": "On the mode of action of eucaryotic initiation factor 1. A factor isolated from the human tonsillar ribosomal wash specifically stimulated the poly (U)-dependent binding of Phe-tRNA to 40S subunits at low Mg2+ concentration and without any requirement for GTP. The stimulated binding of Phe-tRNA to 40S particles was inhibited in proportion to added deacylated tRNA. The factor was inactivated by N-ethylmaleimide, but in the presence of 40S subunits a considerable protection was observed. 40S subunits, incubated with the factor and isolated by centrifugation, carried significant factor activity. The results imply that the human tonsillar factor, which shows a great functional analogy to the eucaryotic initiation factor 1 from other sources, exerts its effect by an initial interaction with the 40S subunit.", "PMID": 964930}
{"id": "pubmed23n0032_6083", "title": "[Aminolysis and racemization of activated esters with the use of high temperatures (author's transl)].", "content": "The reactivities in aminolysis of the benzyl, phenyl, p-nitrophenyl and pentachlorophenyl esters of benzyloxycarbonylphenylalanine were ascertained at different temperatures up to 90 degrees C and the occurrence of racemization investigated. It turned out that the protective function of the benzyloxycarbonyl group in relation to the racemization was conserved, even at the high temperatures used in the peptide synthesis.", "contents": "[Aminolysis and racemization of activated esters with the use of high temperatures (author's transl)]. The reactivities in aminolysis of the benzyl, phenyl, p-nitrophenyl and pentachlorophenyl esters of benzyloxycarbonylphenylalanine were ascertained at different temperatures up to 90 degrees C and the occurrence of racemization investigated. It turned out that the protective function of the benzyloxycarbonyl group in relation to the racemization was conserved, even at the high temperatures used in the peptide synthesis.", "PMID": 964931}
{"id": "pubmed23n0032_6084", "title": "A rapid method for dry weight determination of proteins on a micro scale with an electrobalance.", "content": "A method to determine the dry weight (0.25-2 mg) of aqueous protein solutions within 1 h, using an electrobalance, is described. The drying of 50-200 mul solution pipetted onto a glass fiber disc is carried out in vacuo at 70 degrees C until the recorded dry weight becomes constant (within 25-40 min). It has been shown that the dried residue can subsequently be used for other purposes, such as quantitative amino acid analyses. The method is also suitable for the determination of moisture content in lyophilized protein samples.", "contents": "A rapid method for dry weight determination of proteins on a micro scale with an electrobalance. A method to determine the dry weight (0.25-2 mg) of aqueous protein solutions within 1 h, using an electrobalance, is described. The drying of 50-200 mul solution pipetted onto a glass fiber disc is carried out in vacuo at 70 degrees C until the recorded dry weight becomes constant (within 25-40 min). It has been shown that the dried residue can subsequently be used for other purposes, such as quantitative amino acid analyses. The method is also suitable for the determination of moisture content in lyophilized protein samples.", "PMID": 964932}
{"id": "pubmed23n0032_6085", "title": "Operational dimensions of one multihospital system.", "content": "The pressing realities of health care delivery in the 1970s call for new solutions and fresh approaches to health care administration. The Lutheran Hospitals and Homes Society of America was founded to assist communities that were encountering difficulties in providing for the health care needs of their people. This article describes how the society meets these needs through a multihospital approach in which the \"parent\" organization totally operates its member facilities rather than fragmenting its services by allowing member units to utilize one or more of its services.", "contents": "Operational dimensions of one multihospital system. The pressing realities of health care delivery in the 1970s call for new solutions and fresh approaches to health care administration. The Lutheran Hospitals and Homes Society of America was founded to assist communities that were encountering difficulties in providing for the health care needs of their people. This article describes how the society meets these needs through a multihospital approach in which the \"parent\" organization totally operates its member facilities rather than fragmenting its services by allowing member units to utilize one or more of its services.", "PMID": 964964}
{"id": "pubmed23n0032_6086", "title": "Effectiveness of hospital administrators: a study.", "content": "A survey study of the effectiveness of hospital administrators was conducted by Graduate Studies in Health Services Management, University of Missouri, Columbia. The purpose of the study was to determine whether or not hospital administrators and others knowledgeable about hospital administrators believe that these professionals are effective in their work. In general, administrators, chiefs of staff, and board chairmen rated the administrator's effectiveness higher than did directors of health agencies.", "contents": "Effectiveness of hospital administrators: a study. A survey study of the effectiveness of hospital administrators was conducted by Graduate Studies in Health Services Management, University of Missouri, Columbia. The purpose of the study was to determine whether or not hospital administrators and others knowledgeable about hospital administrators believe that these professionals are effective in their work. In general, administrators, chiefs of staff, and board chairmen rated the administrator's effectiveness higher than did directors of health agencies.", "PMID": 964965}
{"id": "pubmed23n0032_6087", "title": "Fat distribution in the adrenal cortex as an indication of the mode of intrauterine death.", "content": "The presence of fat in the fetal zone of the adrenal cortex in the stillborn macerated fetus is indicative of the mode of intrauterine death. Three basic patterns are recognized. In the type I pattern there is only a scant amount of fat, close to the medullary zone. In the type II pattern fat is more widespread and is present in many more cells of the fetal zone. The type III pattern reflects a massive fatty change of almost all cells throughout the fetal cortical zone. Correlation of the three fat patterns with the pathology of the placenta and clinical data relevant to the etiology of intrauterine death has led to the following conclusions. The type I fat pattern is indicative of an acute mode of death, the type II pattern is indicative of a more prolonged period of intrauterine deterioration prior to death, whereas the type III pattern is indicative of a chronic mode of death. Determination of the fat patterns in the fetal zone of the adrenal cortex can be helpful when the clinician is confronted with the problem of fetal death, especially when maceration may hamper more detailed pathologic studies.", "contents": "Fat distribution in the adrenal cortex as an indication of the mode of intrauterine death. The presence of fat in the fetal zone of the adrenal cortex in the stillborn macerated fetus is indicative of the mode of intrauterine death. Three basic patterns are recognized. In the type I pattern there is only a scant amount of fat, close to the medullary zone. In the type II pattern fat is more widespread and is present in many more cells of the fetal zone. The type III pattern reflects a massive fatty change of almost all cells throughout the fetal cortical zone. Correlation of the three fat patterns with the pathology of the placenta and clinical data relevant to the etiology of intrauterine death has led to the following conclusions. The type I fat pattern is indicative of an acute mode of death, the type II pattern is indicative of a more prolonged period of intrauterine deterioration prior to death, whereas the type III pattern is indicative of a chronic mode of death. Determination of the fat patterns in the fetal zone of the adrenal cortex can be helpful when the clinician is confronted with the problem of fetal death, especially when maceration may hamper more detailed pathologic studies.", "PMID": 964978}
{"id": "pubmed23n0032_6088", "title": "Malignant fibrillo-caveolated cell carcinoma of the human intestinal tract.", "content": "The ultrastructural features of a peculiar midgut carcinoid, containing cytoplasmic filaments, fibrils, and caveolae, are presented. Because of the morphologic similarities between the tumor cells and the recently described intestinal caveolated cell, it is proposed that the cells of the reported carcinoid represent the malignant counterpart of this new type of cell. The name suggested for this variant of a tumor of the enterochromaffin group of intestinal endocrine cells is malignant fibrillocaveolated cell carcinoma.", "contents": "Malignant fibrillo-caveolated cell carcinoma of the human intestinal tract. The ultrastructural features of a peculiar midgut carcinoid, containing cytoplasmic filaments, fibrils, and caveolae, are presented. Because of the morphologic similarities between the tumor cells and the recently described intestinal caveolated cell, it is proposed that the cells of the reported carcinoid represent the malignant counterpart of this new type of cell. The name suggested for this variant of a tumor of the enterochromaffin group of intestinal endocrine cells is malignant fibrillocaveolated cell carcinoma.", "PMID": 964979}
{"id": "pubmed23n0032_6089", "title": "Focal nodular hyperplasia of the liver: a clinicopathologic study and review of the literature.", "content": "We received the clinical records and pathologic material of 20 patients with biopsy proven hepatic focal nodular hyperplasia. The majority of the patients were females of child bearing age, five of whom had a history of oral use of contraceptives. In every instance focal nodular hyperplasia was an incidental finding; liver function tests were always normal. Focal nodular hyperplasia is a distinct histopathologic entity, distinguishable from liver cell adenoma. Specifically it consists of nodular aggregates of cytologically normal hepatocytes with foci of intranodular bile duct proliferation. Focal nodular hyperplasia appears to be a benign entity, even in patients in whom the lesion was not excised. The association between focal nodular hyperplasia and oral use of contraceptives may be coincidental, although hormonally related vascular changes may be responsible for rupture of the lesion.", "contents": "Focal nodular hyperplasia of the liver: a clinicopathologic study and review of the literature. We received the clinical records and pathologic material of 20 patients with biopsy proven hepatic focal nodular hyperplasia. The majority of the patients were females of child bearing age, five of whom had a history of oral use of contraceptives. In every instance focal nodular hyperplasia was an incidental finding; liver function tests were always normal. Focal nodular hyperplasia is a distinct histopathologic entity, distinguishable from liver cell adenoma. Specifically it consists of nodular aggregates of cytologically normal hepatocytes with foci of intranodular bile duct proliferation. Focal nodular hyperplasia appears to be a benign entity, even in patients in whom the lesion was not excised. The association between focal nodular hyperplasia and oral use of contraceptives may be coincidental, although hormonally related vascular changes may be responsible for rupture of the lesion.", "PMID": 964980}
{"id": "pubmed23n0032_6090", "title": "Structural basis of ventricular arrhythmias in human myocardial infarction: a hypothesis.", "content": "The present study was undertaken using light and electron microscopic techniques to determine whether Purkinje fibers survive in the subendocardial region of anteroseptal infarcts in humans. Tissue was obtained for this purpose from 11 patients with 12 documented infarctions at the time of autopsy; six patients died within 72 hours of the infarction and five had healed infarcts. Seven of the 11 patients had ventricular arrhythmias. Light microscopic study indicated that intact cells with a normal appearance remained on the subendocardial surface, although the underlying ventricular muscle either was necrotic or was replaced by fibrous tissue. Electron microscopy demonstrated that these intact surviving cells over the surface of the infarct had few randomly oriented myofibrils, abundant glycogen, and other characteristics of Purkinje fibers. These cells could be readily distinguished from normal or infarcted ventricular muscle cells. Purkinje fibers, the most peripheral part of the conduction system, survive in extensive anteroseptal infarcts and may be the site of origin of ventricular arrhythmias.", "contents": "Structural basis of ventricular arrhythmias in human myocardial infarction: a hypothesis. The present study was undertaken using light and electron microscopic techniques to determine whether Purkinje fibers survive in the subendocardial region of anteroseptal infarcts in humans. Tissue was obtained for this purpose from 11 patients with 12 documented infarctions at the time of autopsy; six patients died within 72 hours of the infarction and five had healed infarcts. Seven of the 11 patients had ventricular arrhythmias. Light microscopic study indicated that intact cells with a normal appearance remained on the subendocardial surface, although the underlying ventricular muscle either was necrotic or was replaced by fibrous tissue. Electron microscopy demonstrated that these intact surviving cells over the surface of the infarct had few randomly oriented myofibrils, abundant glycogen, and other characteristics of Purkinje fibers. These cells could be readily distinguished from normal or infarcted ventricular muscle cells. Purkinje fibers, the most peripheral part of the conduction system, survive in extensive anteroseptal infarcts and may be the site of origin of ventricular arrhythmias.", "PMID": 964981}
{"id": "pubmed23n0032_6091", "title": "Multiple sebaceous adenomas and internal malignant disease: a case report with chromosomal analysis.", "content": "A case of multiple sebaceous adenomas associated with internal malignant disease is further documented. Thirteen similar cases reported in the literature are also reviewed. We support the thesis that this association represents a unique syndrome. It can be seen in both sexes and usually is manifested during the fifth and sixth decades. A family history of malignant disease was revealed among seven of the nine cases mentioned. Of the other skin lesions, keratoacanthoma is also frequently seen. The internal malignant tumors most commonly afflict the gastrointestinal tract, especially the large intestine. With proper treatment the internal tumors appear to be of a low degree of malignancy. However, these patients have a propensity to develop muliple visceral tumors and require periodic follow-up. Some patients develop skin tumors prior to the discovery of internal malignant disease, an event that offers some prognostic value. Chromosomal study of the cultured blood lymphocytes by the Giemsa banding technique failed to reveal any abnormality.", "contents": "Multiple sebaceous adenomas and internal malignant disease: a case report with chromosomal analysis. A case of multiple sebaceous adenomas associated with internal malignant disease is further documented. Thirteen similar cases reported in the literature are also reviewed. We support the thesis that this association represents a unique syndrome. It can be seen in both sexes and usually is manifested during the fifth and sixth decades. A family history of malignant disease was revealed among seven of the nine cases mentioned. Of the other skin lesions, keratoacanthoma is also frequently seen. The internal malignant tumors most commonly afflict the gastrointestinal tract, especially the large intestine. With proper treatment the internal tumors appear to be of a low degree of malignancy. However, these patients have a propensity to develop muliple visceral tumors and require periodic follow-up. Some patients develop skin tumors prior to the discovery of internal malignant disease, an event that offers some prognostic value. Chromosomal study of the cultured blood lymphocytes by the Giemsa banding technique failed to reveal any abnormality.", "PMID": 964982}
{"id": "pubmed23n0032_6092", "title": "A new case of trisomy for the distal part of 13q due to maternal translocation, t(9;13)(p21;q21).", "content": "The first child of a mother with a balanced translocation (9;13) revealed a trisomy for the distal third of 13q. Clinical signs were microcephaly, hemangiomata, long incurved eyelashes, strabismus, enlarged bridge of the nose, abnormally long philtrum, high-arched palate, low set ears, hexadactyly of the four extremities, umbilical and inguinal hernias, neonatal respiratory distress, psychomotor and growth retardation. The proband presented also male pseudohermaphroditism and trigonocephaly. This last trait is the object of a discussion in which cases of partial trisomy 13q cited in the literature are considered for study of the incidence of this dyscephaly in this partial syndrome.", "contents": "A new case of trisomy for the distal part of 13q due to maternal translocation, t(9;13)(p21;q21). The first child of a mother with a balanced translocation (9;13) revealed a trisomy for the distal third of 13q. Clinical signs were microcephaly, hemangiomata, long incurved eyelashes, strabismus, enlarged bridge of the nose, abnormally long philtrum, high-arched palate, low set ears, hexadactyly of the four extremities, umbilical and inguinal hernias, neonatal respiratory distress, psychomotor and growth retardation. The proband presented also male pseudohermaphroditism and trigonocephaly. This last trait is the object of a discussion in which cases of partial trisomy 13q cited in the literature are considered for study of the incidence of this dyscephaly in this partial syndrome.", "PMID": 964983}
{"id": "pubmed23n0032_6093", "title": "Familial C/D translocation t(9;13)(9p23.13q21) in a male associated with recurrent abortion.", "content": "A familial reciprocal translocation, established by R-banding as t(9;13) (9p23;13q21), is described in a phenotypically normal male carrier, whose father is also a balanced carrier and wife had four consecutive spontaneous abortions. The role of translocation in reproductive failure through production of chromosomally unbalanced gametes or by impairment of the spermatogenesis is briefly discussed.", "contents": "Familial C/D translocation t(9;13)(9p23.13q21) in a male associated with recurrent abortion. A familial reciprocal translocation, established by R-banding as t(9;13) (9p23;13q21), is described in a phenotypically normal male carrier, whose father is also a balanced carrier and wife had four consecutive spontaneous abortions. The role of translocation in reproductive failure through production of chromosomally unbalanced gametes or by impairment of the spermatogenesis is briefly discussed.", "PMID": 964984}
{"id": "pubmed23n0032_6094", "title": "Normal meiosis in two 47,XYY men.", "content": "Details of testicular histology and meiosis are given for two 47,XYY men, one an oligospermic childless individual, the other a fertile man with near-normal spermatogenic activity in his testes. Examination of the chromosomes at meiosis, with \"Q\" and \"C\" staining, gave no evidence for the occurrence of the second Y chromosome in the germ line of either individual.", "contents": "Normal meiosis in two 47,XYY men. Details of testicular histology and meiosis are given for two 47,XYY men, one an oligospermic childless individual, the other a fertile man with near-normal spermatogenic activity in his testes. Examination of the chromosomes at meiosis, with \"Q\" and \"C\" staining, gave no evidence for the occurrence of the second Y chromosome in the germ line of either individual.", "PMID": 964985}
{"id": "pubmed23n0032_6095", "title": "Follow-up till age 3--4 of unselected children with sex chromosome abnormalities.", "content": "Follow-up examination of 15 unselected children with aneuploid sex chromosome abnormalities has been made till between the age of 2 1/2 and 5 years. The mental development of the 15 children was in all cases within the normal range, but there was a tendency to some differences compared with their siblings.", "contents": "Follow-up till age 3--4 of unselected children with sex chromosome abnormalities. Follow-up examination of 15 unselected children with aneuploid sex chromosome abnormalities has been made till between the age of 2 1/2 and 5 years. The mental development of the 15 children was in all cases within the normal range, but there was a tendency to some differences compared with their siblings.", "PMID": 964986}
{"id": "pubmed23n0032_6096", "title": "Fine structure of 33 258 H-treated chromosomes.", "content": "Metaphase chromosomes of mouse strain L cells show strikingly uncondensed pericentric heterochromatic regions after treatment of living cells with the benzimidazol-derivative 33 258 Hoechst. In electron micrographs of total preparations after G-band staining the chromosomes are seen to be made up of irregularly folded fibrils of 200-400 A in diameter. In the uncondensed regions only very few fibrils laid loose loops are present, making it probable that only one fibril forms one chromatid.", "contents": "Fine structure of 33 258 H-treated chromosomes. Metaphase chromosomes of mouse strain L cells show strikingly uncondensed pericentric heterochromatic regions after treatment of living cells with the benzimidazol-derivative 33 258 Hoechst. In electron micrographs of total preparations after G-band staining the chromosomes are seen to be made up of irregularly folded fibrils of 200-400 A in diameter. In the uncondensed regions only very few fibrils laid loose loops are present, making it probable that only one fibril forms one chromatid.", "PMID": 964987}
{"id": "pubmed23n0032_6097", "title": "The human leukocyte test system. IX. DNA synthesis and mitoses in PHA-stimulated 2-day cultures set up with Eagle's mimimal essential medium (MEM).", "content": "In human leukocyte cultures set up with Eagle's MEM and stimulated with Difco's PHA M, DNA synthesis and mitotic indices were analyzed by means of 3(H)-thymidine autoradiography and cell counting from 23 up to 52 h after culture initiation. Considerable amounts of DNA synthesis and mitoses were found in this time span. This resembles the results found with Ham's F-10 medium. However, the DNA synthesis pattern and the distribution of mitotic indices a higher yield of asynchrony in Eagle's MEM as compared with Ham's F-10 cultures. Proportions of first, second, and third mitoses at 72 h culture time were determined with different methods.", "contents": "The human leukocyte test system. IX. DNA synthesis and mitoses in PHA-stimulated 2-day cultures set up with Eagle's mimimal essential medium (MEM). In human leukocyte cultures set up with Eagle's MEM and stimulated with Difco's PHA M, DNA synthesis and mitotic indices were analyzed by means of 3(H)-thymidine autoradiography and cell counting from 23 up to 52 h after culture initiation. Considerable amounts of DNA synthesis and mitoses were found in this time span. This resembles the results found with Ham's F-10 medium. However, the DNA synthesis pattern and the distribution of mitotic indices a higher yield of asynchrony in Eagle's MEM as compared with Ham's F-10 cultures. Proportions of first, second, and third mitoses at 72 h culture time were determined with different methods.", "PMID": 964988}
{"id": "pubmed23n0032_6098", "title": "Bf polymorphism: study of a new variant (F0.55).", "content": "A variant band in the Bf polymorphism has been found in the serum of a healthy women, her mother, her sister, and two brothers. By direct comparison the band was found to migrate faster than F but slower than F1. It is highly probable that the band represents a new allele at the Bf locus, Bf 0.55. The new allele was transmitted in this family together with the HLA haplotype A11, B27.", "contents": "Bf polymorphism: study of a new variant (F0.55). A variant band in the Bf polymorphism has been found in the serum of a healthy women, her mother, her sister, and two brothers. By direct comparison the band was found to migrate faster than F but slower than F1. It is highly probable that the band represents a new allele at the Bf locus, Bf 0.55. The new allele was transmitted in this family together with the HLA haplotype A11, B27.", "PMID": 964989}
{"id": "pubmed23n0032_6099", "title": "Heterogeneity of Dyggve-Melchior-Clausen dwarfism.", "content": "Sibs with apparent Dyggve-Melchior-Clausen (DMC) dwarfism and normal intelligence are described. Three other familial and 3 sporadic cases with DMC dwarfism and normal intelligence are known. Twelve familial and 9 sporadic cases are known with the usual combination of DMC dwarfism and severe mental retardation. Since the two conditions appear to breed true they seem to be genetically different. We propose to name the former \"Smith-McCort dwarfism\" to clearly distinguish it from the DMC syndrome in which mental retardation is a constituent part. Both conditions are inherited as autosomal recessive traits. Spinal cord compression due to atlantoaxial instability is a serious and preventable complication of both disorders.", "contents": "Heterogeneity of Dyggve-Melchior-Clausen dwarfism. Sibs with apparent Dyggve-Melchior-Clausen (DMC) dwarfism and normal intelligence are described. Three other familial and 3 sporadic cases with DMC dwarfism and normal intelligence are known. Twelve familial and 9 sporadic cases are known with the usual combination of DMC dwarfism and severe mental retardation. Since the two conditions appear to breed true they seem to be genetically different. We propose to name the former \"Smith-McCort dwarfism\" to clearly distinguish it from the DMC syndrome in which mental retardation is a constituent part. Both conditions are inherited as autosomal recessive traits. Spinal cord compression due to atlantoaxial instability is a serious and preventable complication of both disorders.", "PMID": 964990}
{"id": "pubmed23n0032_6100", "title": "Polymorphism of properdin factor B in South African Negroid, Indian and Colored populations.", "content": "Properdin factor B phenotypes were determined in 7 Bantu speaking Negroid populations, 1 Indian, and 1 Colored population of South Africa in a total of 1258 individuals. In the Negroid populations allele frequencies were: BfF 0.655, BfS 0.282, BfRARE 0.063, in the Indian population: BfF 0.322, BfS 0.645, BfRARE 0.033, and in the Colored population: BfF 0.513, BfS 0.435, BfRARE 0.052. In addition, 2 so far unknown F alleles and possibly 1 new S allele were discovered.", "contents": "Polymorphism of properdin factor B in South African Negroid, Indian and Colored populations. Properdin factor B phenotypes were determined in 7 Bantu speaking Negroid populations, 1 Indian, and 1 Colored population of South Africa in a total of 1258 individuals. In the Negroid populations allele frequencies were: BfF 0.655, BfS 0.282, BfRARE 0.063, in the Indian population: BfF 0.322, BfS 0.645, BfRARE 0.033, and in the Colored population: BfF 0.513, BfS 0.435, BfRARE 0.052. In addition, 2 so far unknown F alleles and possibly 1 new S allele were discovered.", "PMID": 964991}
{"id": "pubmed23n0032_6101", "title": "A familial tetraphocomelia syndrome involving limb deformities, cleft lip, cleft palate, and associated anomalies--a new syndrome.", "content": "This paper reports a rare malformation syndrome which is observed in two sibs (brother and sister) of a family. It consists of nearly symmetric reductive defects of the limbs, flexon contractures of various joints, cleft lip and cleft palate, multiple minor abnormalities including capillary hemangioma of the forehead, hypoplastic cartilages of ears and nose, micrognathia, intrauterine growth retardation, and possibly mental retardation. Chromosomes of both parents and propositi are normal. Genetic data suggest autosomal recessive inheritance.", "contents": "A familial tetraphocomelia syndrome involving limb deformities, cleft lip, cleft palate, and associated anomalies--a new syndrome. This paper reports a rare malformation syndrome which is observed in two sibs (brother and sister) of a family. It consists of nearly symmetric reductive defects of the limbs, flexon contractures of various joints, cleft lip and cleft palate, multiple minor abnormalities including capillary hemangioma of the forehead, hypoplastic cartilages of ears and nose, micrognathia, intrauterine growth retardation, and possibly mental retardation. Chromosomes of both parents and propositi are normal. Genetic data suggest autosomal recessive inheritance.", "PMID": 964992}
{"id": "pubmed23n0032_6102", "title": "Unbalanced 13q/21q translocation: a revised study of the case previously reported as 21-monosomy.", "content": "Reexamination was made on a male infant previously reported as 21-monosomy. Extensive chromosome banding analyses in the patient and parents disclosed an unbalanced de novo translocation between chromosomes 13 and 21. The patient's karyotype was interpreted as 45,XY,--13,--21+der(13),t(13;21) (q2 or 3;q1 or 2)pat. The patient showed many clinical features characteristic of 13q--syndrome.", "contents": "Unbalanced 13q/21q translocation: a revised study of the case previously reported as 21-monosomy. Reexamination was made on a male infant previously reported as 21-monosomy. Extensive chromosome banding analyses in the patient and parents disclosed an unbalanced de novo translocation between chromosomes 13 and 21. The patient's karyotype was interpreted as 45,XY,--13,--21+der(13),t(13;21) (q2 or 3;q1 or 2)pat. The patient showed many clinical features characteristic of 13q--syndrome.", "PMID": 964993}
{"id": "pubmed23n0032_6103", "title": "Tertiary trisomy, 47,XX,+14q--, resulting from maternal balanced translocation, 46,XX,t(14;16)(q11;q24).", "content": "A 3-year-old child with tertiary trisomy (14 (+14q--), daughter of a mother with a balanced reciprocal translocation [46,XX,t(14;16)(q11;q24) is presented. Craniostenosis and developmental retardation were the primary presenting features in this patient.", "contents": "Tertiary trisomy, 47,XX,+14q--, resulting from maternal balanced translocation, 46,XX,t(14;16)(q11;q24). A 3-year-old child with tertiary trisomy (14 (+14q--), daughter of a mother with a balanced reciprocal translocation [46,XX,t(14;16)(q11;q24) is presented. Craniostenosis and developmental retardation were the primary presenting features in this patient.", "PMID": 964994}
{"id": "pubmed23n0032_6104", "title": "Center for Barr body condensation. A case of Turner's syndrome with 45,X/46,X,dic(X) (Xqter leads to p22::p22 leads to qter).", "content": "An 11-year-old girl with karyotype 45,X/46,X,dic(X) (Xqter leads to p22::p22 leads to qter) is presented. The abnormal X is always found to be the inactive and late replicating X, and according to previous investigations by Therman et al. (1974) part of the cells are seen to have bipartite Barr bodies.", "contents": "Center for Barr body condensation. A case of Turner's syndrome with 45,X/46,X,dic(X) (Xqter leads to p22::p22 leads to qter). An 11-year-old girl with karyotype 45,X/46,X,dic(X) (Xqter leads to p22::p22 leads to qter) is presented. The abnormal X is always found to be the inactive and late replicating X, and according to previous investigations by Therman et al. (1974) part of the cells are seen to have bipartite Barr bodies.", "PMID": 964996}
{"id": "pubmed23n0032_6105", "title": "Partial trisomy 4q syndrome: case report and review.", "content": "This communication contributes an additional case of partial trisomy for the long arm of chromosome 4[46,XX,t(X;4)(Q27;Q25)]. Three generations of the patient's family were karyotyped and her mother and brother were found to be balanced translocation carriers. From the patient's clinical examination and from review of 13 similar cases reported in the literature, the following phenotype emerged: constant features were psychomotor retardation, muscle hypotonia at birth, undescended testes in all males and abnormal auricles, typically with a prominent antitragus. Anomalies of kidneys and other structures of the urinary system were present consistently as probably the most characteristic feature of the syndrome. Over 200 structural anomalies of other systems were reported, many of the common to other autosomal chromosomal syndromes.", "contents": "Partial trisomy 4q syndrome: case report and review. This communication contributes an additional case of partial trisomy for the long arm of chromosome 4[46,XX,t(X;4)(Q27;Q25)]. Three generations of the patient's family were karyotyped and her mother and brother were found to be balanced translocation carriers. From the patient's clinical examination and from review of 13 similar cases reported in the literature, the following phenotype emerged: constant features were psychomotor retardation, muscle hypotonia at birth, undescended testes in all males and abnormal auricles, typically with a prominent antitragus. Anomalies of kidneys and other structures of the urinary system were present consistently as probably the most characteristic feature of the syndrome. Over 200 structural anomalies of other systems were reported, many of the common to other autosomal chromosomal syndromes.", "PMID": 964997}
{"id": "pubmed23n0032_6106", "title": "A 46,XY, del(18)(pter leads to p1 100:) cebocephalic child from a 46,XX,t(12;18)(18pter leads to 18 p 1100:: 12qter leads to 12pter) normal parent.", "content": "A normal female with an 18p--chromosome but without any feature of the 18p--syndrome produced a cebocephalic child whose karyotype included an 18p--chromosome. Evidence is presented that the normal female is a nonreciprocal translocation heterozygote resulting from the short arm of one chromosome 18 becoming attached to the long arm of a chromosome 12.", "contents": "A 46,XY, del(18)(pter leads to p1 100:) cebocephalic child from a 46,XX,t(12;18)(18pter leads to 18 p 1100:: 12qter leads to 12pter) normal parent. A normal female with an 18p--chromosome but without any feature of the 18p--syndrome produced a cebocephalic child whose karyotype included an 18p--chromosome. Evidence is presented that the normal female is a nonreciprocal translocation heterozygote resulting from the short arm of one chromosome 18 becoming attached to the long arm of a chromosome 12.", "PMID": 964998}
{"id": "pubmed23n0032_6107", "title": "Acromesomelic dwarfism: description of a patient and comparison with previously reported cases.", "content": "A woman is described who has a severe bone dysplasia confined to the limbs. The degree of involvement is more marked in the distal bones. Her forearms and legs are short; the fibulae are represented by distal triangular remnants; the metacarpals are short, several phalanges in the fingers are absent; and the toes are represented by \"ball-like\" remnants containing a single bone. The findings in our patient are compared to previously reported cases of acromesomelic dwarfism.", "contents": "Acromesomelic dwarfism: description of a patient and comparison with previously reported cases. A woman is described who has a severe bone dysplasia confined to the limbs. The degree of involvement is more marked in the distal bones. Her forearms and legs are short; the fibulae are represented by distal triangular remnants; the metacarpals are short, several phalanges in the fingers are absent; and the toes are represented by \"ball-like\" remnants containing a single bone. The findings in our patient are compared to previously reported cases of acromesomelic dwarfism.", "PMID": 964999}
{"id": "pubmed23n0032_6108", "title": "Nucleolus organizer and satellite association in a variant D-group chromosome.", "content": "A No. 15 chromosome with a short arm longer than usual is observed in two phenotypically normal brothers. This chromosome appears to have no visible satellite, shows no N-band staining, and is never involved in satellite association. These results have led us to the conclusion that this chromosome lacks the nucleolus organizer region.", "contents": "Nucleolus organizer and satellite association in a variant D-group chromosome. A No. 15 chromosome with a short arm longer than usual is observed in two phenotypically normal brothers. This chromosome appears to have no visible satellite, shows no N-band staining, and is never involved in satellite association. These results have led us to the conclusion that this chromosome lacks the nucleolus organizer region.", "PMID": 965001}
{"id": "pubmed23n0032_6109", "title": "A new, high frequency variant of alpha1-antitrypsin.", "content": "A new variant of alpha1-antitrypsin has been detected with the aid of isoelectric focusing on polyacrylamide slab gels. In contrast with many other variants this new alpha1-antitrypsin allele is found in 10-15% of the phenotypes examined. The electrophoretic properties of the new alpha1-antitrypsin variant in isofocusing polyacrylamide gels differ only slightly from the most common alpha1-antitrypsin allele M. On the basis of its electroforetic behaviour we propose the term MN to indicate this new protease inhibitor variant. The isofocusing technique employed, provides an easy to handle, very reproducible method for determining the alpha1-antitrypsin phenotype and can be employed for large scale screening.", "contents": "A new, high frequency variant of alpha1-antitrypsin. A new variant of alpha1-antitrypsin has been detected with the aid of isoelectric focusing on polyacrylamide slab gels. In contrast with many other variants this new alpha1-antitrypsin allele is found in 10-15% of the phenotypes examined. The electrophoretic properties of the new alpha1-antitrypsin variant in isofocusing polyacrylamide gels differ only slightly from the most common alpha1-antitrypsin allele M. On the basis of its electroforetic behaviour we propose the term MN to indicate this new protease inhibitor variant. The isofocusing technique employed, provides an easy to handle, very reproducible method for determining the alpha1-antitrypsin phenotype and can be employed for large scale screening.", "PMID": 965002}
{"id": "pubmed23n0032_6110", "title": "Assignment of a gene for human mitochondrial isocitrate dehydrogenase (ICD-M, EC 1.1.1.41) to chromosome 15.", "content": "Segregation analysis of human biochemical markers and chromosomes in human-mouse somatic cell hybrids allowed to demonstrate synteny of ICDM with the genes for phosphomannose isomerase and pyruvate kinase and to assign the linkage group to human chromosome 15.", "contents": "Assignment of a gene for human mitochondrial isocitrate dehydrogenase (ICD-M, EC 1.1.1.41) to chromosome 15. Segregation analysis of human biochemical markers and chromosomes in human-mouse somatic cell hybrids allowed to demonstrate synteny of ICDM with the genes for phosphomannose isomerase and pyruvate kinase and to assign the linkage group to human chromosome 15.", "PMID": 965003}
{"id": "pubmed23n0032_6111", "title": "Rapid detection of glyoxalase I (GLO) on cellulose acetate gel and the distribution of GLO variants in a Dutch population.", "content": "A rapid electrophoretic procedure is described for detecting the human red cell glyoxalase I variants (GLO 1, GLO 2-1, and GLO 2) on cellulose acetate gel (cellogel) on which the sites of enzymed activity are visualized as purple bands against white background. The frequency of GLO1 gene in a Dutch population living in and around Leiden was found to be 0.4544.", "contents": "Rapid detection of glyoxalase I (GLO) on cellulose acetate gel and the distribution of GLO variants in a Dutch population. A rapid electrophoretic procedure is described for detecting the human red cell glyoxalase I variants (GLO 1, GLO 2-1, and GLO 2) on cellulose acetate gel (cellogel) on which the sites of enzymed activity are visualized as purple bands against white background. The frequency of GLO1 gene in a Dutch population living in and around Leiden was found to be 0.4544.", "PMID": 965004}
{"id": "pubmed23n0032_6112", "title": "Persistence of high intestinal lactase activity (lactose tolerance) in Afghanistan.", "content": "Two hundred and seventy apparently healthy adult subjects from Afghanistan, mainly from the central and eastern parts of the country, were subjected to a lactose tolerance test. The change of blood glucose from the fasting concentration at 20 min after the administration of lactose showed a bimodal distribution. Forty-seven subjects had a rise of blood glucose concentration of more than 1.1 mmol/l and were classified as persistence of high intestinal lactase activity (PHILA), a term which lays emphasis on the fact that high lactase activity in the adult is an unusual state whose prevalence in some populations requires explanation. In the Afghan sample there were no significant differences of the frequency of PHILA in different ethnic groups.", "contents": "Persistence of high intestinal lactase activity (lactose tolerance) in Afghanistan. Two hundred and seventy apparently healthy adult subjects from Afghanistan, mainly from the central and eastern parts of the country, were subjected to a lactose tolerance test. The change of blood glucose from the fasting concentration at 20 min after the administration of lactose showed a bimodal distribution. Forty-seven subjects had a rise of blood glucose concentration of more than 1.1 mmol/l and were classified as persistence of high intestinal lactase activity (PHILA), a term which lays emphasis on the fact that high lactase activity in the adult is an unusual state whose prevalence in some populations requires explanation. In the Afghan sample there were no significant differences of the frequency of PHILA in different ethnic groups.", "PMID": 965005}
{"id": "pubmed23n0032_6113", "title": "ESD polymorphism in Norway.", "content": "ESD phenotype distribution and allele frequencies in 217 Norwegians and 196 Norwegian Lapps are presented. There is good accordance with Hardy-Weinberg distribution, ESD1 allele frequencies are 0.887 in the Norwegians and 0.872 in the Lapps.", "contents": "ESD polymorphism in Norway. ESD phenotype distribution and allele frequencies in 217 Norwegians and 196 Norwegian Lapps are presented. There is good accordance with Hardy-Weinberg distribution, ESD1 allele frequencies are 0.887 in the Norwegians and 0.872 in the Lapps.", "PMID": 965006}
{"id": "pubmed23n0032_6114", "title": "Premature chromosome condensation in irradiated man.", "content": "Micronuclei and premature chromosome condensation previously described in mammalian cells exposed to different types of irradiation and chemicals in vitro or in vivo could be demonstrated in cultured lymphocytes derived from chronically low-dose irradiated persons. The usefulness of these findings for biological recording of irradiation effects is suggested.", "contents": "Premature chromosome condensation in irradiated man. Micronuclei and premature chromosome condensation previously described in mammalian cells exposed to different types of irradiation and chemicals in vitro or in vivo could be demonstrated in cultured lymphocytes derived from chronically low-dose irradiated persons. The usefulness of these findings for biological recording of irradiation effects is suggested.", "PMID": 965007}
{"id": "pubmed23n0032_6115", "title": "[A further chromosome analysis in induced abortions (author's transl)].", "content": "The cytogenetic data of 500 unpublished cases of induced abortions are reported. The incidence of gross chromosome abnormalities was 7.4% (37/500). The frequencies of chromosome abnormalities after 6 gestational weeks decreased gradually with advancing the gestational weeks, while the frequency in the group of 5 gestational weeks remained considerably high as 25.0%.", "contents": "[A further chromosome analysis in induced abortions (author's transl)]. The cytogenetic data of 500 unpublished cases of induced abortions are reported. The incidence of gross chromosome abnormalities was 7.4% (37/500). The frequencies of chromosome abnormalities after 6 gestational weeks decreased gradually with advancing the gestational weeks, while the frequency in the group of 5 gestational weeks remained considerably high as 25.0%.", "PMID": 965008}
{"id": "pubmed23n0032_6116", "title": "Leukocyte peroxidase in Spielmeyer-Vogt's disease.", "content": "Leukocyte peroxidase activity was estimated in 5 patients with the juvenile form of neuronal ceroid lipofuscinosis (Spielmeyer-Vogt's disease) and in 15 healthy controls. In contradiction to recent reports normal activity of p-phenylene diamine mediated peroxidase was found in the patients. The possible role of contamination of the white cell preparation with hemoglobin is discussed.", "contents": "Leukocyte peroxidase in Spielmeyer-Vogt's disease. Leukocyte peroxidase activity was estimated in 5 patients with the juvenile form of neuronal ceroid lipofuscinosis (Spielmeyer-Vogt's disease) and in 15 healthy controls. In contradiction to recent reports normal activity of p-phenylene diamine mediated peroxidase was found in the patients. The possible role of contamination of the white cell preparation with hemoglobin is discussed.", "PMID": 965009}
{"id": "pubmed23n0032_6117", "title": "A case of trisomy for the short arm of chromosome no. 9(+9(p)).", "content": "A new case of \"free\" trisomy for the short arm of No. 9 chromosome identified by Giemsa staining and \"Giemsa-11 technique\" is reported.", "contents": "A case of trisomy for the short arm of chromosome no. 9(+9(p)). A new case of \"free\" trisomy for the short arm of No. 9 chromosome identified by Giemsa staining and \"Giemsa-11 technique\" is reported.", "PMID": 965010}
{"id": "pubmed23n0032_6118", "title": "Familial 13p+ chromosome with mental retardation and dysmorphic features of two children.", "content": "Chromosomal studies of two brothers with mental retardation and dysmorphic features showed a 13p+ chromosome. The same 13p+ chromosome was found in the father and the other sib, who were both phenotypically normal. The relationship between the physical abnormalities and the chromosomal findings are discussed.", "contents": "Familial 13p+ chromosome with mental retardation and dysmorphic features of two children. Chromosomal studies of two brothers with mental retardation and dysmorphic features showed a 13p+ chromosome. The same 13p+ chromosome was found in the father and the other sib, who were both phenotypically normal. The relationship between the physical abnormalities and the chromosomal findings are discussed.", "PMID": 965011}
{"id": "pubmed23n0032_6119", "title": "Deletion of the short arms of chromosome 20.", "content": "A 46, XX, del(20) (p11) karyotype (Paris Conference, 1971) was identified in an 11-month-old French-Canadian girl with a dysmorphic syndrome, multiple congenital anomalies, psychomotor and growth retardation. Both parents had normal phenotype and karyotype.", "contents": "Deletion of the short arms of chromosome 20. A 46, XX, del(20) (p11) karyotype (Paris Conference, 1971) was identified in an 11-month-old French-Canadian girl with a dysmorphic syndrome, multiple congenital anomalies, psychomotor and growth retardation. Both parents had normal phenotype and karyotype.", "PMID": 965012}
{"id": "pubmed23n0032_6120", "title": "The different actions of normal and supranormal calcium concentrations on the proliferation of BALB/c 3T3 mouse cells.", "content": "In the presence of a normal (1.25 to 1.80 mM) calcium concentration, addition of fresh bovine calf serum or completely changing the medium induces proliferatively quiescent BALB/c 3T3 mouse cells in dense cultures to start a growth division cycle and initiate DNA synthesis about 12 hr later. Fresh, low-calcium (0.02 mM physiologically available) medium also causes cells to start a growth-division cycle. Howver, the development of such stimulated, calcium-deprived cells tops just before the expected time of initiation of DNA synthesis, which can then be rapidly induced by restoration of the normal calcium concentration. Simply raising the calcium concentration to nonphysiologically high levels (without otherwise altering the medium) can mimic the action of fresh serum or fresh whole medium by inducing some of the cells in proliferatively quiescent confluent concluent cultures to start a growth-division cycle and initiate DNA synthesis 22 hr later.", "contents": "The different actions of normal and supranormal calcium concentrations on the proliferation of BALB/c 3T3 mouse cells. In the presence of a normal (1.25 to 1.80 mM) calcium concentration, addition of fresh bovine calf serum or completely changing the medium induces proliferatively quiescent BALB/c 3T3 mouse cells in dense cultures to start a growth division cycle and initiate DNA synthesis about 12 hr later. Fresh, low-calcium (0.02 mM physiologically available) medium also causes cells to start a growth-division cycle. Howver, the development of such stimulated, calcium-deprived cells tops just before the expected time of initiation of DNA synthesis, which can then be rapidly induced by restoration of the normal calcium concentration. Simply raising the calcium concentration to nonphysiologically high levels (without otherwise altering the medium) can mimic the action of fresh serum or fresh whole medium by inducing some of the cells in proliferatively quiescent confluent concluent cultures to start a growth-division cycle and initiate DNA synthesis 22 hr later.", "PMID": 965015}
{"id": "pubmed23n0032_6121", "title": "The role of the gas phase in the greening and growth of illuminated cell suspension cultures of spinach (Spinacia oleracea, L.).", "content": "The gas phase developed above spinach suspension cultures critically affected their growth and greening. Ethylene accumulation inhibited greening; this effect of ethylene was antagonised when the culture gas phase was enriched with carbon dioxide.  Greening was enhanced by reducing the partial pressure of oxygen below the air level; this effect was observed when oxygen supply did not restrict growth.", "contents": "The role of the gas phase in the greening and growth of illuminated cell suspension cultures of spinach (Spinacia oleracea, L.). The gas phase developed above spinach suspension cultures critically affected their growth and greening. Ethylene accumulation inhibited greening; this effect of ethylene was antagonised when the culture gas phase was enriched with carbon dioxide.  Greening was enhanced by reducing the partial pressure of oxygen below the air level; this effect was observed when oxygen supply did not restrict growth.", "PMID": 965016}
{"id": "pubmed23n0032_6122", "title": "Changes in soluble proteins in callus cells of hypersensitive tobacco inoculated with tobacco mosaic virus.", "content": "Protein changes occurred in callus cells of hypersensitive tobacco (Nicotiana tabacum var. Xanthi-nc) 72 hr after inoculation with tobacco mosaic virus and incubation on a minimal growth medium. Two protein bands, serologically related to viral coat protein, were obtained from extracts of infected cells following electrophoresis on 7% and 10% polyacrylamide gels. An additional, slower migrating protein, perhaps due to virus-induced stimulation of a host protein, also was detected. Although local lesions appeared on callus after 40 hr of incubation, four proteins previously reported in lesion-bearing hypersensitive tobacco leaves were not found. The possible significance of this and the usefulness of a callus-TMV system as a tool to study virus-induced protein changes are discussed.", "contents": "Changes in soluble proteins in callus cells of hypersensitive tobacco inoculated with tobacco mosaic virus. Protein changes occurred in callus cells of hypersensitive tobacco (Nicotiana tabacum var. Xanthi-nc) 72 hr after inoculation with tobacco mosaic virus and incubation on a minimal growth medium. Two protein bands, serologically related to viral coat protein, were obtained from extracts of infected cells following electrophoresis on 7% and 10% polyacrylamide gels. An additional, slower migrating protein, perhaps due to virus-induced stimulation of a host protein, also was detected. Although local lesions appeared on callus after 40 hr of incubation, four proteins previously reported in lesion-bearing hypersensitive tobacco leaves were not found. The possible significance of this and the usefulness of a callus-TMV system as a tool to study virus-induced protein changes are discussed.", "PMID": 965017}
{"id": "pubmed23n0032_6123", "title": "[Measles and its complications in Hamburg from 1960 to 1973 (author's transl)].", "content": "A study of measles was undertaken from the case histories of 3 093 children under 15 years of age, treated in the children's clinics of Hamburg between 1960 to 1973. 562 children were suffering from pneumonia (18,2%), 230 had otitis (7,4%) and 229 (7,4%) showed neural affections (143 cases with fits and 86 patients with encephalopathy or encephalitis), Various parameters were analysed: age, sex, seasonal distribution, fatality, age disposition to convulsions and measles encephalitis, \"incubation period\" of neural affections, sequelae. Permanent sequelae due to measles encephalitis were seen in 14% of the cases. A plea is made for an universal immunisation against measles.", "contents": "[Measles and its complications in Hamburg from 1960 to 1973 (author's transl)]. A study of measles was undertaken from the case histories of 3 093 children under 15 years of age, treated in the children's clinics of Hamburg between 1960 to 1973. 562 children were suffering from pneumonia (18,2%), 230 had otitis (7,4%) and 229 (7,4%) showed neural affections (143 cases with fits and 86 patients with encephalopathy or encephalitis), Various parameters were analysed: age, sex, seasonal distribution, fatality, age disposition to convulsions and measles encephalitis, \"incubation period\" of neural affections, sequelae. Permanent sequelae due to measles encephalitis were seen in 14% of the cases. A plea is made for an universal immunisation against measles.", "PMID": 965033}
{"id": "pubmed23n0032_6124", "title": "[Seasonal patterns of coronavirus infections in Hamburg].", "content": "During a serological surveillance programme between October 1974 and September 1975 in Hamburg, 1624 sera from all age groups of the population were examined for the presence of coronavirus antibodies (OC 43 agent). 61,51% of the sera showed HI-titres between 1:8 and 1:128. The geometric mean antiboty titre (GMT) for the whole group was 1:12,2. The serological seasonal pattern suggests that coronaviruses are circulating the whole year in the urban population with a prevalence in the cold and a decrease in the warm season (June). The highest incidence of seropositive reactions (82,9%) was found in the 15-24 years old individuals (GMT 1:13,8). These data indicate an increased spread of coronaviruses in this segment of the population and a marked susceptibility of the group.", "contents": "[Seasonal patterns of coronavirus infections in Hamburg]. During a serological surveillance programme between October 1974 and September 1975 in Hamburg, 1624 sera from all age groups of the population were examined for the presence of coronavirus antibodies (OC 43 agent). 61,51% of the sera showed HI-titres between 1:8 and 1:128. The geometric mean antiboty titre (GMT) for the whole group was 1:12,2. The serological seasonal pattern suggests that coronaviruses are circulating the whole year in the urban population with a prevalence in the cold and a decrease in the warm season (June). The highest incidence of seropositive reactions (82,9%) was found in the 15-24 years old individuals (GMT 1:13,8). These data indicate an increased spread of coronaviruses in this segment of the population and a marked susceptibility of the group.", "PMID": 965034}
{"id": "pubmed23n0032_6125", "title": "[Identification of dextrans using rapid fluid chromatography].", "content": "It is reported about an analytical method for routine control of clinical dextrans, a method which is considerably quicker than the previously used ones. It is based on the application of HPLC (high pressure liquid chromatography) to permeation chromatography of dextrans on controlled pore glass beads (CPG). The time required for a permeation chromatogram of a dextran sample could be reduced to less than one hour. Calibration curves, necessary for the evaluation of the chromatograms, were established by use of Pharmacia-Testdextrans.", "contents": "[Identification of dextrans using rapid fluid chromatography]. It is reported about an analytical method for routine control of clinical dextrans, a method which is considerably quicker than the previously used ones. It is based on the application of HPLC (high pressure liquid chromatography) to permeation chromatography of dextrans on controlled pore glass beads (CPG). The time required for a permeation chromatogram of a dextran sample could be reduced to less than one hour. Calibration curves, necessary for the evaluation of the chromatograms, were established by use of Pharmacia-Testdextrans.", "PMID": 965079}
{"id": "pubmed23n0032_6126", "title": "[New system for no-wait continuous infusion of several different solutions using an additional apparatus].", "content": "This article introduces a simple coordination system for two or more infusion liquids. Solutions drip down one after the other in a continuous flow, with no attendance required, two or more infusion containers being connected in a way described below. There is no intermingling of solutions when using non-rigid containers, and only a slight one when using glass or plastic material. The check valves work through the pressure exerted by the columns of liquid, the containers being placed on different levels. The essential advantages are: less discomfort and less danger for patients and staff. The system can be used for a washing drainage.", "contents": "[New system for no-wait continuous infusion of several different solutions using an additional apparatus]. This article introduces a simple coordination system for two or more infusion liquids. Solutions drip down one after the other in a continuous flow, with no attendance required, two or more infusion containers being connected in a way described below. There is no intermingling of solutions when using non-rigid containers, and only a slight one when using glass or plastic material. The check valves work through the pressure exerted by the columns of liquid, the containers being placed on different levels. The essential advantages are: less discomfort and less danger for patients and staff. The system can be used for a washing drainage.", "PMID": 965080}
{"id": "pubmed23n0032_6127", "title": "Effect of glucose on the activity of feeding centres before and after parasagittal cuts between medial and lateral hypothalamus.", "content": "Intracarotid administration of isotonic glucose (0.5 ml of 5.4%) in the starving albino rats produced an increase in the multiunit activity (MUA) of ventromedial hypothalamus (satiety centre) and a decrease in the MUA of the lateral hypothalamic area (feeding centre). Intracarotid infusion of normal saline did not change the MUA of any of these centres. Parasagittal knife cuts placed in between the satiety and feeding centres did not affect the responsiveness of these centres to glucose administration as indicated by the recorded multiunit activity.", "contents": "Effect of glucose on the activity of feeding centres before and after parasagittal cuts between medial and lateral hypothalamus. Intracarotid administration of isotonic glucose (0.5 ml of 5.4%) in the starving albino rats produced an increase in the multiunit activity (MUA) of ventromedial hypothalamus (satiety centre) and a decrease in the MUA of the lateral hypothalamic area (feeding centre). Intracarotid infusion of normal saline did not change the MUA of any of these centres. Parasagittal knife cuts placed in between the satiety and feeding centres did not affect the responsiveness of these centres to glucose administration as indicated by the recorded multiunit activity.", "PMID": 965075}
{"id": "pubmed23n0032_6128", "title": "[Osmolality problems].", "content": "This is a survey about all factors influencing the osmotic pressure. The actual osmotic pressure in the plasma of healthy individuals is regulated essentially by the natrium ions and the corresponding anions. The one exception is the dysfunctioning diabetes mellitus with distinctly advanced hyperglycaemia. Syndromes with an accompanying azotemia demonstrate a seeming hyperosmolality as long as the osmotic pressure is measured or calculated by the customary method. The real and present osmotic condition can only be calculated when the blood urea concentration is no longer considered. It is proposed that the term \"corrected osmolality\" be used for the values determined in this manner.", "contents": "[Osmolality problems]. This is a survey about all factors influencing the osmotic pressure. The actual osmotic pressure in the plasma of healthy individuals is regulated essentially by the natrium ions and the corresponding anions. The one exception is the dysfunctioning diabetes mellitus with distinctly advanced hyperglycaemia. Syndromes with an accompanying azotemia demonstrate a seeming hyperosmolality as long as the osmotic pressure is measured or calculated by the customary method. The real and present osmotic condition can only be calculated when the blood urea concentration is no longer considered. It is proposed that the term \"corrected osmolality\" be used for the values determined in this manner.", "PMID": 965081}
{"id": "pubmed23n0032_6129", "title": "A comparative study of several methods for recording spontaneous motor activity in mice under drug effects.", "content": "Various existing methods of recording motor activity in animals are briefly reviewed. An electronic adaptation of an old one, the Jiggle cage, is described. The relative advantages and demerits in recording behaviour of mice under drug effects are compared with those of the traditional Jiggle cage, an electromagnetic activity counter and observational ratings.", "contents": "A comparative study of several methods for recording spontaneous motor activity in mice under drug effects. Various existing methods of recording motor activity in animals are briefly reviewed. An electronic adaptation of an old one, the Jiggle cage, is described. The relative advantages and demerits in recording behaviour of mice under drug effects are compared with those of the traditional Jiggle cage, an electromagnetic activity counter and observational ratings.", "PMID": 965076}
{"id": "pubmed23n0032_6130", "title": "In vitro anthelmintic action of some indigenous medicinal plants on Ascardia galli worms.", "content": "Different parts of ten indigenous medicinal plants were screened for their in vitro anthelmintic activity against Ascaridia galli worms of the birds. Preparations from Carica papaya, Sapindus trifoliatus, Butea frondosa and Momordica charantia were more effective than piperazine hexahydrate.", "contents": "In vitro anthelmintic action of some indigenous medicinal plants on Ascardia galli worms. Different parts of ten indigenous medicinal plants were screened for their in vitro anthelmintic activity against Ascaridia galli worms of the birds. Preparations from Carica papaya, Sapindus trifoliatus, Butea frondosa and Momordica charantia were more effective than piperazine hexahydrate.", "PMID": 965077}
{"id": "pubmed23n0032_6131", "title": "[Nutrition problems in kidney diseases].", "content": "The practice of dietetic therapy is unusual today for patients suffering from renal failure without hypertension and reduction of glomerular filtration rate. Specific treatment is needed, however, for arterial hypertension, uremia, calculus and uralith disease. Experiments in rats showed, that a lot of uremic symptoms following poorly functioning kidneys are partly at least caused by disturbances in amino acid metabolism. Uremia patients with dysfunctioning plasma protein metabolism (transferrin, complement, cholinesterase, prealbumin and retinolbinding protein) need oral, respectively parenteral substitution of essential amino acids. This substitution is very important under catabolic stress conditions in uremic syndrome with and without vividialysis treatment.", "contents": "[Nutrition problems in kidney diseases]. The practice of dietetic therapy is unusual today for patients suffering from renal failure without hypertension and reduction of glomerular filtration rate. Specific treatment is needed, however, for arterial hypertension, uremia, calculus and uralith disease. Experiments in rats showed, that a lot of uremic symptoms following poorly functioning kidneys are partly at least caused by disturbances in amino acid metabolism. Uremia patients with dysfunctioning plasma protein metabolism (transferrin, complement, cholinesterase, prealbumin and retinolbinding protein) need oral, respectively parenteral substitution of essential amino acids. This substitution is very important under catabolic stress conditions in uremic syndrome with and without vividialysis treatment.", "PMID": 965082}
{"id": "pubmed23n0032_6132", "title": "[Effect of canrenoate potassium on intraoperative disturbances of electrolyte equilibrium].", "content": "In 61 patients the influence of canrenoate potassium on the shift of electrolytes during the intraoperative phase is studied. Without medication of canrenoate potassium depletion of potassium, magnesium and zinc occurs, a sign of transitory intraoperative hyperaldosteronism. On the other hand, preoperative and intraoperative infusion of 1000 mg canrenoate potassium prevents an increased excretion of electrolytes. Causes and consequences of the electrolyte depletion as well as the effect of canrenoate-potassium medication are discussed.", "contents": "[Effect of canrenoate potassium on intraoperative disturbances of electrolyte equilibrium]. In 61 patients the influence of canrenoate potassium on the shift of electrolytes during the intraoperative phase is studied. Without medication of canrenoate potassium depletion of potassium, magnesium and zinc occurs, a sign of transitory intraoperative hyperaldosteronism. On the other hand, preoperative and intraoperative infusion of 1000 mg canrenoate potassium prevents an increased excretion of electrolytes. Causes and consequences of the electrolyte depletion as well as the effect of canrenoate-potassium medication are discussed.", "PMID": 965083}
{"id": "pubmed23n0032_6133", "title": "Ability of temperature-sensitive mutants of the recombinant influenza S/N (H2N1) virus to induce immunity to parental (H0N1 and H2N2) viruses.", "content": "The behavior in mice of two thermosensitive (ts) mutants (denoted ts217 and ts700) of the recombinant influenza virus S/N (H2N1) was studied. The parental thermoresistant (tr) virus and both of the mutants were capable of inducing protection against pneumotropic A/Singapore (H2N2) and A/WS (H0N1) challenge viruses. Immunity against the Singapore virus, with which the S/N virus shared the hemagglutinin, developed earlier than against the WS virus, with which the S/N virus shared the neuraminidase. The tr and ts217 viruses were immunologically more active than the ts700 virus. The first two viruses grew markedly better in mouse lungs than did the latter. In the course of ts217 virus replication in vivo, revertants capable of growing at 39 degrees C appeared readily. On the other hand, the ts700 virus proved to be genetically stable. These data seem to provide evidence of a linkage between the stability of the ts phenotype, reproductive capacity in mouse lungs, and immunogenicity in the viruses examined.", "contents": "Ability of temperature-sensitive mutants of the recombinant influenza S/N (H2N1) virus to induce immunity to parental (H0N1 and H2N2) viruses. The behavior in mice of two thermosensitive (ts) mutants (denoted ts217 and ts700) of the recombinant influenza virus S/N (H2N1) was studied. The parental thermoresistant (tr) virus and both of the mutants were capable of inducing protection against pneumotropic A/Singapore (H2N2) and A/WS (H0N1) challenge viruses. Immunity against the Singapore virus, with which the S/N virus shared the hemagglutinin, developed earlier than against the WS virus, with which the S/N virus shared the neuraminidase. The tr and ts217 viruses were immunologically more active than the ts700 virus. The first two viruses grew markedly better in mouse lungs than did the latter. In the course of ts217 virus replication in vivo, revertants capable of growing at 39 degrees C appeared readily. On the other hand, the ts700 virus proved to be genetically stable. These data seem to provide evidence of a linkage between the stability of the ts phenotype, reproductive capacity in mouse lungs, and immunogenicity in the viruses examined.", "PMID": 965084}
{"id": "pubmed23n0032_6134", "title": "Comparison of replication of adenovirus type 2 and type 4 in human lymphocyte cultures.", "content": "Adenovirus type 2 was capable of replicating in purified lymphocyte cultures from human adenoid specimens. Phytohemagglutinin stimulation enhanced the replication of virus. Viral titers of 103 to 104 50% tissue culture infective doses per ml were reached after 4 to 8 days. Only 1 to 3 per 106 cells were found to produce virus. In contrast, there was no evidence that lymphocyte cultures could support the replication of adenovirus type 4. The life span of cultures infected with type 2 or 4 was not reduced. The possibility that lymphocytes infected with virus play a role in initiating natural, persisting adenovirus infections of human adenoids is discussed.", "contents": "Comparison of replication of adenovirus type 2 and type 4 in human lymphocyte cultures. Adenovirus type 2 was capable of replicating in purified lymphocyte cultures from human adenoid specimens. Phytohemagglutinin stimulation enhanced the replication of virus. Viral titers of 103 to 104 50% tissue culture infective doses per ml were reached after 4 to 8 days. Only 1 to 3 per 106 cells were found to produce virus. In contrast, there was no evidence that lymphocyte cultures could support the replication of adenovirus type 4. The life span of cultures infected with type 2 or 4 was not reduced. The possibility that lymphocytes infected with virus play a role in initiating natural, persisting adenovirus infections of human adenoids is discussed.", "PMID": 965085}
{"id": "pubmed23n0032_6135", "title": "Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.", "content": "Preparative polyacrylamide gel electrophoresis was used to isolate individual components of an alkaline-soluble-water-soluble fraction of the cell wall of Blastomyces dermatitidis yeast phase. One component isolated demonstrated exceptional specificity and reactivity when tested on guinea pigs infected with B. dermatitidis. This component displayed no cross-reactivity when tested on animals infected with Histoplasma capsulatum. The significance of isolation of a purified, specific antigen is discussed.", "contents": "Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall. Preparative polyacrylamide gel electrophoresis was used to isolate individual components of an alkaline-soluble-water-soluble fraction of the cell wall of Blastomyces dermatitidis yeast phase. One component isolated demonstrated exceptional specificity and reactivity when tested on guinea pigs infected with B. dermatitidis. This component displayed no cross-reactivity when tested on animals infected with Histoplasma capsulatum. The significance of isolation of a purified, specific antigen is discussed.", "PMID": 965086}
{"id": "pubmed23n0032_6136", "title": "Significant contribution of spleen cells in mediating the lethal effects of endotoxin in vivo.", "content": "Two closely related, histocompatible mouse strains that have marked differences in both in vitro and in vivo responses to endotoxin were used to evaluate the contribution of lymphoid cells to the lethal effect of endotoxin. C3H/HeJ mice are endotoxin resistant, whereas C3H/HeN mice are endotoxin sensitive. In vitro spleen cell mitogenic responses to endotoxin were similar in untreated mice and in mice that received sublethal irradiation (450 R) followed by reconstitution with autologous spleen cells. Reconstitution with spleen cells from the related strain produced chimeric animals with spleen cell mitogenic activity like that of the donor strain. When chimeric animals were subjected to a lethal challenge of endotoxin, their response was markedly altered by the transferred lymphoid cells. C3H/HeJ animals reconstituted with C3H/HeN cells became more endotoxin sensitive, whereas C3H/HeJ cells became more endotoxin resistant. These results indicate that spleen cells play a significant, detrimental role in endotoxin-induced lethality.", "contents": "Significant contribution of spleen cells in mediating the lethal effects of endotoxin in vivo. Two closely related, histocompatible mouse strains that have marked differences in both in vitro and in vivo responses to endotoxin were used to evaluate the contribution of lymphoid cells to the lethal effect of endotoxin. C3H/HeJ mice are endotoxin resistant, whereas C3H/HeN mice are endotoxin sensitive. In vitro spleen cell mitogenic responses to endotoxin were similar in untreated mice and in mice that received sublethal irradiation (450 R) followed by reconstitution with autologous spleen cells. Reconstitution with spleen cells from the related strain produced chimeric animals with spleen cell mitogenic activity like that of the donor strain. When chimeric animals were subjected to a lethal challenge of endotoxin, their response was markedly altered by the transferred lymphoid cells. C3H/HeJ animals reconstituted with C3H/HeN cells became more endotoxin sensitive, whereas C3H/HeJ cells became more endotoxin resistant. These results indicate that spleen cells play a significant, detrimental role in endotoxin-induced lethality.", "PMID": 965087}
{"id": "pubmed23n0032_6137", "title": "Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system.", "content": "Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion. Hepatitis B antigen (HBAg)-associated deoxyribonucleic acid (DNA) polymerase activity was specifically determined by the difference in incorporation of [methyl-3H]thymidine 5' -triphosphate into DNA by an aliquot of centrifuged serum samples after it had been treated either with normal rabbit serum or with rabbit antibody to HBSAg. All of 58 serum samples containing e antigen revealed HBAg-associated DNA polymerase activity, whereas none of 96 samples containing anti-e did. In the remaining 249 samples in which neither e antigen nor anti-e was found, 62 showed specific DNA polymerase activity, although at lower levels than the samples containing e antigen.", "contents": "Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system. Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion. Hepatitis B antigen (HBAg)-associated deoxyribonucleic acid (DNA) polymerase activity was specifically determined by the difference in incorporation of [methyl-3H]thymidine 5' -triphosphate into DNA by an aliquot of centrifuged serum samples after it had been treated either with normal rabbit serum or with rabbit antibody to HBSAg. All of 58 serum samples containing e antigen revealed HBAg-associated DNA polymerase activity, whereas none of 96 samples containing anti-e did. In the remaining 249 samples in which neither e antigen nor anti-e was found, 62 showed specific DNA polymerase activity, although at lower levels than the samples containing e antigen.", "PMID": 965088}
{"id": "pubmed23n0032_6138", "title": "Immunological relationships between glucosyltransferases from Streptococcus mutans serotypes.", "content": "Partially purified glycosyltransferase enzymes for Streptococcus mutans GS-5 (serotype c) have been utilized to prepare antibodies directed against the soluble glucan-synthesizing activity, GTF-B, and the insoluble-soluble glucan synthetic activity, GTF-A. Anti-GTF-A inhibited insoluble glucan formation catalyzed by the extracellular enzymes from strains GS-5 and FA-1 (serotype b) to a much greater extent than that of strains HS-6 (serotype a) or OMZ-176 (serotype d). This antibody fraction also inhibited both the cell-associated glucosyltransferase activities as well as the sucrose-mediated adherence of cells to glass surfaces by strains GS-5 and FA-1 but not that of strains HS-6 and OMZ-176. Anti-GTF-B inhibited soluble glucan formation catalyzed by the extracellular enzymes of strains GS-5 but not that of strain HS-6, FA-1, or OMZ-176. However, this antibody fraction did not strongly inhibit either the cell-associated glycosyltransferase activity or cellular adherence of any of the four strains. These results with body antibody fractions were also correlated with the ability  of the antibodies to agglutinate the cells and form precipitin bands after immunodiffusion with the extracellular enzymes. Antibody prepared against the homogeneous soluble glucan-synthesizing enzyme demonstrated similar effects to the anti-GTF-B fraction. These results are discussed in terms of the antigenic relationships existing between the glucosyltransferases from different serotypes of S. mutans.", "contents": "Immunological relationships between glucosyltransferases from Streptococcus mutans serotypes. Partially purified glycosyltransferase enzymes for Streptococcus mutans GS-5 (serotype c) have been utilized to prepare antibodies directed against the soluble glucan-synthesizing activity, GTF-B, and the insoluble-soluble glucan synthetic activity, GTF-A. Anti-GTF-A inhibited insoluble glucan formation catalyzed by the extracellular enzymes from strains GS-5 and FA-1 (serotype b) to a much greater extent than that of strains HS-6 (serotype a) or OMZ-176 (serotype d). This antibody fraction also inhibited both the cell-associated glucosyltransferase activities as well as the sucrose-mediated adherence of cells to glass surfaces by strains GS-5 and FA-1 but not that of strains HS-6 and OMZ-176. Anti-GTF-B inhibited soluble glucan formation catalyzed by the extracellular enzymes of strains GS-5 but not that of strain HS-6, FA-1, or OMZ-176. However, this antibody fraction did not strongly inhibit either the cell-associated glycosyltransferase activity or cellular adherence of any of the four strains. These results with body antibody fractions were also correlated with the ability  of the antibodies to agglutinate the cells and form precipitin bands after immunodiffusion with the extracellular enzymes. Antibody prepared against the homogeneous soluble glucan-synthesizing enzyme demonstrated similar effects to the anti-GTF-B fraction. These results are discussed in terms of the antigenic relationships existing between the glucosyltransferases from different serotypes of S. mutans.", "PMID": 965089}
{"id": "pubmed23n0032_6139", "title": "Requirements for ingestion of Chlamydia psittaci by mouse fibroblasts (L cells).", "content": "Ingestion of 14C-amino acid-labeled Chlamydia psittaci (6BC) by mouse fibroblasts (L cells) was inhibited when the host cells were incubated for 30 min at 37 degrees C in Earle salts containing 10 mug of crystalline trypsin per ml. Tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. Trypsin-treated L cells almost completely recovered their ability to ingest chlamydiae after 4 h at 37 degrees C in medium 199 with 5% fetal calf serum. Cycloheximide (10 mug/ml) blocked this recovery. Heating 14C-amino acid-labeled C. psittaci for 3 min at 60 degrees C inhibited its ingestion by L cells, whereas inactivating it with ultraviolet light was without effect on the ingestion rate. These results show that efficient ingestion of C. psittaci by L cells involves trypsin-labile sites on the host and heat-sensitive sites on the parasite. The failure of excess unlabeled infectious C. psittaci to promote the ingestion of 14C-labeled heat-inactivated chlamydiae suggests that direct interaction between these two sites must occur for uptake to proceed normally.", "contents": "Requirements for ingestion of Chlamydia psittaci by mouse fibroblasts (L cells). Ingestion of 14C-amino acid-labeled Chlamydia psittaci (6BC) by mouse fibroblasts (L cells) was inhibited when the host cells were incubated for 30 min at 37 degrees C in Earle salts containing 10 mug of crystalline trypsin per ml. Tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. Trypsin-treated L cells almost completely recovered their ability to ingest chlamydiae after 4 h at 37 degrees C in medium 199 with 5% fetal calf serum. Cycloheximide (10 mug/ml) blocked this recovery. Heating 14C-amino acid-labeled C. psittaci for 3 min at 60 degrees C inhibited its ingestion by L cells, whereas inactivating it with ultraviolet light was without effect on the ingestion rate. These results show that efficient ingestion of C. psittaci by L cells involves trypsin-labile sites on the host and heat-sensitive sites on the parasite. The failure of excess unlabeled infectious C. psittaci to promote the ingestion of 14C-labeled heat-inactivated chlamydiae suggests that direct interaction between these two sites must occur for uptake to proceed normally.", "PMID": 965090}
{"id": "pubmed23n0032_6140", "title": "Serological and genetic examination of some nontypical Streptococcus mutans strains.", "content": "Thirty-four strains of Streptococcus mutans whose antigenic or genetic positions were unclear or unknown with respect to the serological scheme of Bratthall (1970) and Perch et al. (1974), or the genetic (deoxyribonucleic acid base sequence homology) scheme of Coykendall were analyzed to clarify their relationship to previously well-characterized strains. Strain OMZ175 of the \"new\" serotype f was genetically homologous with strains of S. mutans subsp. mutans. Strains of the \"new\" serotype g were homologous with serotype d strains (S. mutans subsp. sobrinus). Strains isolated from wild rats constituted a new genetic group but carried the c antigen. Thus, strains within a \"genospecies\" (subspecies) of S. mutans may not always carry a unique or characteristic antigen. We suggest that the existence of multiple serotypes within subspecies represents antigenic variation and adaptations to hosts.", "contents": "Serological and genetic examination of some nontypical Streptococcus mutans strains. Thirty-four strains of Streptococcus mutans whose antigenic or genetic positions were unclear or unknown with respect to the serological scheme of Bratthall (1970) and Perch et al. (1974), or the genetic (deoxyribonucleic acid base sequence homology) scheme of Coykendall were analyzed to clarify their relationship to previously well-characterized strains. Strain OMZ175 of the \"new\" serotype f was genetically homologous with strains of S. mutans subsp. mutans. Strains of the \"new\" serotype g were homologous with serotype d strains (S. mutans subsp. sobrinus). Strains isolated from wild rats constituted a new genetic group but carried the c antigen. Thus, strains within a \"genospecies\" (subspecies) of S. mutans may not always carry a unique or characteristic antigen. We suggest that the existence of multiple serotypes within subspecies represents antigenic variation and adaptations to hosts.", "PMID": 965091}
{"id": "pubmed23n0032_6141", "title": "Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures.", "content": "A modified purification method was used to isolate the neurotoxin of proteolytic Clostridium botulinum type B strain Lamanna. The preparation was found to be a mixture of two protein forms. They were of molecular weight 152,000 and could not be separated by ion-exchange chromatography or electrophoresis in polyacrylamide gel. One was a single polypeptide chain, and the other was a dichain molecule (nicked toxin) held together by an interchain disulfide bond(s). Trypsinization increased the toxicity of the toxin preparation and converted the single-chain molecules into dichain forms that were indistinguishable from the endogenously generated nicked toxin. A protease of the type B culture, with substrate specificity similar to that of trypsin, did not change detectably the molecular form of unnicked type E toxin, although toxicity was increased. Higher toxicity was obtained when unnicked type E was trypsinized; the resulting preparation contained only nicked toxin molecules.", "contents": "Molecular forms of neurotoxins in proteolytic Clostridium botulinum type B cultures. A modified purification method was used to isolate the neurotoxin of proteolytic Clostridium botulinum type B strain Lamanna. The preparation was found to be a mixture of two protein forms. They were of molecular weight 152,000 and could not be separated by ion-exchange chromatography or electrophoresis in polyacrylamide gel. One was a single polypeptide chain, and the other was a dichain molecule (nicked toxin) held together by an interchain disulfide bond(s). Trypsinization increased the toxicity of the toxin preparation and converted the single-chain molecules into dichain forms that were indistinguishable from the endogenously generated nicked toxin. A protease of the type B culture, with substrate specificity similar to that of trypsin, did not change detectably the molecular form of unnicked type E toxin, although toxicity was increased. Higher toxicity was obtained when unnicked type E was trypsinized; the resulting preparation contained only nicked toxin molecules.", "PMID": 965092}
{"id": "pubmed23n0032_6142", "title": "Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans Serovar pomona.", "content": "Mice not normally susceptible to infection with Leptospira interrogans serovar pomona were rendered susceptible to lethal infections by treatment with a single dose of 300 mg of cyclophosphamide (Cy) per kg administered optimally from 4 days before to 1 day after infection. Cy-treated mice with either passively or actively acquired antibody were protected from death. Blood levels of leptospires in infected untreated and in Cy-treated mice remained similar until 2 days after infection, when untreated mice cleared the leptospires. Soon afterwards, opsonizing and agglutinating antibody appeared. Cy-treated mice given spleen cells from other normal or specifically immune mice were protected from infection. An important factor in the natural resistance of mice to leptospiral infection appears to be their capacity to produce circulating antibody within 48 to 72 h. Applications are suggested for this animal model in vaccination and protection studies.", "contents": "Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans Serovar pomona. Mice not normally susceptible to infection with Leptospira interrogans serovar pomona were rendered susceptible to lethal infections by treatment with a single dose of 300 mg of cyclophosphamide (Cy) per kg administered optimally from 4 days before to 1 day after infection. Cy-treated mice with either passively or actively acquired antibody were protected from death. Blood levels of leptospires in infected untreated and in Cy-treated mice remained similar until 2 days after infection, when untreated mice cleared the leptospires. Soon afterwards, opsonizing and agglutinating antibody appeared. Cy-treated mice given spleen cells from other normal or specifically immune mice were protected from infection. An important factor in the natural resistance of mice to leptospiral infection appears to be their capacity to produce circulating antibody within 48 to 72 h. Applications are suggested for this animal model in vaccination and protection studies.", "PMID": 965093}
{"id": "pubmed23n0032_6143", "title": "Use of semisolid agar from initiation of pure Bacteroides fragilis infection in mice.", "content": "The development of a pure Bacteroides fragilis infection in mice is described. The infection produces large subcutaneous abscesses at the site of injection which can be observed grossly within 7 days after injection. The infection was initiated by infection of pure cultures grown in semisolid agar medium. Similar infections were also produced with pure cultures of B. distasonis, B. ovatus, B. thetaiotaomicron, and B. vulgatus. However, a distinct deoxyribonucleic acid homology group, formerly classified as B. thetaiotaomicron, did not produce abscesses in any of the mice tested.", "contents": "Use of semisolid agar from initiation of pure Bacteroides fragilis infection in mice. The development of a pure Bacteroides fragilis infection in mice is described. The infection produces large subcutaneous abscesses at the site of injection which can be observed grossly within 7 days after injection. The infection was initiated by infection of pure cultures grown in semisolid agar medium. Similar infections were also produced with pure cultures of B. distasonis, B. ovatus, B. thetaiotaomicron, and B. vulgatus. However, a distinct deoxyribonucleic acid homology group, formerly classified as B. thetaiotaomicron, did not produce abscesses in any of the mice tested.", "PMID": 965094}
{"id": "pubmed23n0032_6144", "title": "Ultrastructural and biochemical alterations effected by viridin B, a bacterocin of alpha-hemolytic streptococci.", "content": "Viridin B, a bacteriocin produced by Streptococcus mitis strain 42885, was shown previously to exert a bactericidal effect against a Neisseria sicca strain but only a bacteriostatic effect against a coagulase-negative staphylococcus. In the present report, this dual effect was defined further. Viridin B caused the appearance of mesosomes in both indicator strains early in the course of exposure. Whereas mesosomes were the only noticeable alterations in the staphylococcal strain at all times of exposure to bacteriocin, progressive changes occurred in the N. sicca cells involving the nucleoid and cytoplasmic contents and resulting in the appearance of bacterial ghosts. Biochemical studies indicated a rapid and complete cessation of the incorporation of [3H]thymidine in N. sicca and in the staphylococcus. Whereas the incorporation of [3H]leucine and [3H]uridine in N. sicca was completely stopped, such incorporation was only reduced in the staphylococcus.", "contents": "Ultrastructural and biochemical alterations effected by viridin B, a bacterocin of alpha-hemolytic streptococci. Viridin B, a bacteriocin produced by Streptococcus mitis strain 42885, was shown previously to exert a bactericidal effect against a Neisseria sicca strain but only a bacteriostatic effect against a coagulase-negative staphylococcus. In the present report, this dual effect was defined further. Viridin B caused the appearance of mesosomes in both indicator strains early in the course of exposure. Whereas mesosomes were the only noticeable alterations in the staphylococcal strain at all times of exposure to bacteriocin, progressive changes occurred in the N. sicca cells involving the nucleoid and cytoplasmic contents and resulting in the appearance of bacterial ghosts. Biochemical studies indicated a rapid and complete cessation of the incorporation of [3H]thymidine in N. sicca and in the staphylococcus. Whereas the incorporation of [3H]leucine and [3H]uridine in N. sicca was completely stopped, such incorporation was only reduced in the staphylococcus.", "PMID": 965095}
{"id": "pubmed23n0032_6145", "title": "Relationship of bacteriophages to alpha toxin production in Clostridium novyi types A and B.", "content": "The relationship of specific bacteriophages to the production of the lethal alpha toxin in Clostridium novyi types A and B was investigated. When type A strain 5771 reverted to the phage-sensitive state, it ceased to produce alpha toxin but continued to produce the gamma and epsilon antigens. This \"nontoxigenic\" culture, therefore, more closely resembled C. botulinum types C and D than the other C. novyi types. Phage-sensitive type B strains also ceased to produce the alpha toxin but continued to produce the beta toxin, and therefore very colesly resembled C. novyi type D (C. haemolyticum). Alpha toxin was again produced when the phage-sensitive cultures were reinfected with the respective tox+ phages. Alpha toxin production could also be induced in the \"nontoxigenic\" phage-sensitive derivatives from type B strain 8024 by tox+ phages isolated from other strains of type B. tox- phages were also isolated, but they did not affect alpha toxin production. The tox+ phages also caused a marked change in the colonial morphology of type B strains. In this report we present evidence that alpha toxin production by C. novyi type A strain 5771 and type B strain 8024 depends upon the continued presence and participation of specific bacteriophages designated as NA1tox+ and NB1tox+, respectively.", "contents": "Relationship of bacteriophages to alpha toxin production in Clostridium novyi types A and B. The relationship of specific bacteriophages to the production of the lethal alpha toxin in Clostridium novyi types A and B was investigated. When type A strain 5771 reverted to the phage-sensitive state, it ceased to produce alpha toxin but continued to produce the gamma and epsilon antigens. This \"nontoxigenic\" culture, therefore, more closely resembled C. botulinum types C and D than the other C. novyi types. Phage-sensitive type B strains also ceased to produce the alpha toxin but continued to produce the beta toxin, and therefore very colesly resembled C. novyi type D (C. haemolyticum). Alpha toxin was again produced when the phage-sensitive cultures were reinfected with the respective tox+ phages. Alpha toxin production could also be induced in the \"nontoxigenic\" phage-sensitive derivatives from type B strain 8024 by tox+ phages isolated from other strains of type B. tox- phages were also isolated, but they did not affect alpha toxin production. The tox+ phages also caused a marked change in the colonial morphology of type B strains. In this report we present evidence that alpha toxin production by C. novyi type A strain 5771 and type B strain 8024 depends upon the continued presence and participation of specific bacteriophages designated as NA1tox+ and NB1tox+, respectively.", "PMID": 965096}
{"id": "pubmed23n0032_6146", "title": "Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals.", "content": "The reovirus-like particles present in the feces of young pigs and foals with acute enteritis and the virus causing epizootic diarrhea of infant mice were found to be indistinguishable morphologically from each other, from the South African SA. 11 and \"O\" viruses, and from the rotaviruses of children and calves. The inner capsid layer of each of these viruses reacted seriologically with sera of children, calves, mice, piglets, and foals convalescent from infection with their respective rotaviruses. These sera reacted by immunofluorescence with human, bovine, porcine, and murine rotaviruses, SA.11, and \"O\" viruses in tissue cultures and with human bovine, procine, nad murine viral antigens by complement fixation and gel diffusion. However, the antisera differed in their ability to react serologically with the outer capsid layer of the viruses investigated and in their ability to neutralize tissue culture-adapted calf virus. These two tests may demonstrate strain or host specificity among rotaviruses. Since the porcine, murine, and equine viruses are closely related serologically to and are morphologically identical to the human and bovine viruses, they should be included in the group of viruses for which the term \"rotavirus\" has been suggested. All known members of this proposed group of viruses share a common antigen, probably situated within the inner capsid layer; thus, any one of the viruses may be used for the preparation of antigen or antibody for diagnostic tests, and this will aid in the diagnosis of virus infection in those species from which a rotavirus has not been cultured.", "contents": "Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. The reovirus-like particles present in the feces of young pigs and foals with acute enteritis and the virus causing epizootic diarrhea of infant mice were found to be indistinguishable morphologically from each other, from the South African SA. 11 and \"O\" viruses, and from the rotaviruses of children and calves. The inner capsid layer of each of these viruses reacted seriologically with sera of children, calves, mice, piglets, and foals convalescent from infection with their respective rotaviruses. These sera reacted by immunofluorescence with human, bovine, porcine, and murine rotaviruses, SA.11, and \"O\" viruses in tissue cultures and with human bovine, procine, nad murine viral antigens by complement fixation and gel diffusion. However, the antisera differed in their ability to react serologically with the outer capsid layer of the viruses investigated and in their ability to neutralize tissue culture-adapted calf virus. These two tests may demonstrate strain or host specificity among rotaviruses. Since the porcine, murine, and equine viruses are closely related serologically to and are morphologically identical to the human and bovine viruses, they should be included in the group of viruses for which the term \"rotavirus\" has been suggested. All known members of this proposed group of viruses share a common antigen, probably situated within the inner capsid layer; thus, any one of the viruses may be used for the preparation of antigen or antibody for diagnostic tests, and this will aid in the diagnosis of virus infection in those species from which a rotavirus has not been cultured.", "PMID": 965097}
{"id": "pubmed23n0032_6147", "title": "Reovirus-like agent associated with fatal diarrhea in neonatal pigs.", "content": "Large numbers of a reovirus-like agent were visualized with electron microscopy in bacteria-free gut homogenates obtained from piglets with a fatal diarrhea resembling transmissible gastroenteritis. The syndrome, of vomiting, diarrhea, dehydration, and death, was reproduced in piglets artificially infected with these bacteria-free gut homogenates. Reovirus-like particles persisted in serial piglet passage and none was seen in uninfected, asymptomatic controls. Hyperimmune sera (made in recovered piglets) aggregated the reovirus-like particles, as judged by immunoelectron microscopy, and neutralized the infectious agent. The cytoplasm in enterocytes on infected intestinal epithelium fluoresced when this hyperimmune sera was used in an indirect fluorescent antibody test. Feeding cow colostrum or diets containing porcine gamma globulin protected infected piglets. No cytopathogenic effect was noted in infected tissue cultures, nor did this agent affect neonatal guinea pigs, hamsters, mice, and rats. The agent did not agglutinate human O or A erythrocytes.", "contents": "Reovirus-like agent associated with fatal diarrhea in neonatal pigs. Large numbers of a reovirus-like agent were visualized with electron microscopy in bacteria-free gut homogenates obtained from piglets with a fatal diarrhea resembling transmissible gastroenteritis. The syndrome, of vomiting, diarrhea, dehydration, and death, was reproduced in piglets artificially infected with these bacteria-free gut homogenates. Reovirus-like particles persisted in serial piglet passage and none was seen in uninfected, asymptomatic controls. Hyperimmune sera (made in recovered piglets) aggregated the reovirus-like particles, as judged by immunoelectron microscopy, and neutralized the infectious agent. The cytoplasm in enterocytes on infected intestinal epithelium fluoresced when this hyperimmune sera was used in an indirect fluorescent antibody test. Feeding cow colostrum or diets containing porcine gamma globulin protected infected piglets. No cytopathogenic effect was noted in infected tissue cultures, nor did this agent affect neonatal guinea pigs, hamsters, mice, and rats. The agent did not agglutinate human O or A erythrocytes.", "PMID": 965098}
{"id": "pubmed23n0032_6148", "title": "H and M antigens of Histoplasma capsulatum: preparation of antisera and location of these antigens in yeast-phase cells.", "content": "Antiserum has been prepared in rabbits against the H and M antigens of H. capsulatum with immunoelectrophoretic precipitin arcs used as vaccines. The antiserum is specific for H. capsulatum in the immunodiffusion test and can be used as reference serum for identifying antibodies to these antigens in sera from suspected cases histoplasmosis. We found that (1) hand m antigens are not located on the surface of yeast-phase cells and (ii) complement fixation releases the antigens reactive in the complement fixation test from yeast-phase cells.", "contents": "H and M antigens of Histoplasma capsulatum: preparation of antisera and location of these antigens in yeast-phase cells. Antiserum has been prepared in rabbits against the H and M antigens of H. capsulatum with immunoelectrophoretic precipitin arcs used as vaccines. The antiserum is specific for H. capsulatum in the immunodiffusion test and can be used as reference serum for identifying antibodies to these antigens in sera from suspected cases histoplasmosis. We found that (1) hand m antigens are not located on the surface of yeast-phase cells and (ii) complement fixation releases the antigens reactive in the complement fixation test from yeast-phase cells.", "PMID": 965099}
{"id": "pubmed23n0032_6149", "title": "Effect of aeromonas salmonicida (furunculosis) infection on the incorporation of [1-14C]acetate into lipids of trout liver.", "content": "[1-(14)C]acetate is incorporated into lipids more rapidly by liver from Aeromonas salmonicida-infected trout than by normal trout liver, both in vitro and in vivo. The increase was, in general, about twofold to less than threefold.", "contents": "Effect of aeromonas salmonicida (furunculosis) infection on the incorporation of [1-14C]acetate into lipids of trout liver. [1-(14)C]acetate is incorporated into lipids more rapidly by liver from Aeromonas salmonicida-infected trout than by normal trout liver, both in vitro and in vivo. The increase was, in general, about twofold to less than threefold.", "PMID": 965100}
{"id": "pubmed23n0032_6150", "title": "Comparison of the in vitro bactericidal activity of human serum and leukocytes against bacteroides fragilis and Fusobacterium mortiferum in aerobic and anaerobic environments.", "content": "In aerobic and anaerobic in vitro environments, Fusobacterium mortiferum was killed by human leukocytes or serum alone or in combination; clinical isolates of Bacteroides fragilis were killed only by leukocytes in the presence of serum.", "contents": "Comparison of the in vitro bactericidal activity of human serum and leukocytes against bacteroides fragilis and Fusobacterium mortiferum in aerobic and anaerobic environments. In aerobic and anaerobic in vitro environments, Fusobacterium mortiferum was killed by human leukocytes or serum alone or in combination; clinical isolates of Bacteroides fragilis were killed only by leukocytes in the presence of serum.", "PMID": 965101}
{"id": "pubmed23n0032_6151", "title": "Binding of morphine by serum globulins from morphine-treated rabbits. II. Antibody nature of the binding globulins.", "content": "Data are presented which show that the increased binding of morphine by sera from rabbits which had been treated with morphine in an appropriate manner is mediated by immunoglobulins and is specific. These observations, together with findings reported in the preceding paper, suggest that morphine or one of its derivatives can evoke specific immune responses. It seems most likely that the response is evoked by a complex, formed in vivo, of a tissue or serum protein and morphine or one of its metabolites.", "contents": "Binding of morphine by serum globulins from morphine-treated rabbits. II. Antibody nature of the binding globulins. Data are presented which show that the increased binding of morphine by sera from rabbits which had been treated with morphine in an appropriate manner is mediated by immunoglobulins and is specific. These observations, together with findings reported in the preceding paper, suggest that morphine or one of its derivatives can evoke specific immune responses. It seems most likely that the response is evoked by a complex, formed in vivo, of a tissue or serum protein and morphine or one of its metabolites.", "PMID": 965102}
{"id": "pubmed23n0032_6152", "title": "Characteristics of guinea pig IgE.", "content": "Anti-dinitrophenyl (DNP) IgE antibody was produced in guinea pigs (GP) by immunization and repeated boosters with 1 mug DNP-Ascaris extract in A1 (OH)3. The isoelectric point was determined to be 6.48 and the molecular weight to be 185,000 daltons. In absorbtion chromatography, rabbit anti-GPIgG1 could not absorb the anaphylactic activity of the IgE. The activity of IgE was destroyed by heating at 56 degrees C for 1 h. IgE persisted in the skin for at least 3 weeks.", "contents": "Characteristics of guinea pig IgE. Anti-dinitrophenyl (DNP) IgE antibody was produced in guinea pigs (GP) by immunization and repeated boosters with 1 mug DNP-Ascaris extract in A1 (OH)3. The isoelectric point was determined to be 6.48 and the molecular weight to be 185,000 daltons. In absorbtion chromatography, rabbit anti-GPIgG1 could not absorb the anaphylactic activity of the IgE. The activity of IgE was destroyed by heating at 56 degrees C for 1 h. IgE persisted in the skin for at least 3 weeks.", "PMID": 965103}
{"id": "pubmed23n0032_6153", "title": "Bovine reaginic antibody. I. Rat mast cell degranulation by bovine allergic serum.", "content": "A method of utilizing morphological changes in rat mast cells to determine reaginic antibody activity in bovine serum is described. This technique, which has been shown to be useful for the diagnosis of allergies in man, relies on the ability of antigen to degranulate mast cells sensitized with allergic serum. Experiments with radioactively-labelled allergic bovine globulin indicated the specificity of the binding of such proteins to rat mast cells. Cross-reaction between reaginic bovine antibody and human IgE was shown by a binding assay involving the uptake of 125I-labelled anti-human IgE globulin by mast cells incubated with bovine passive cutaneous anaphylaxis positive globulin.", "contents": "Bovine reaginic antibody. I. Rat mast cell degranulation by bovine allergic serum. A method of utilizing morphological changes in rat mast cells to determine reaginic antibody activity in bovine serum is described. This technique, which has been shown to be useful for the diagnosis of allergies in man, relies on the ability of antigen to degranulate mast cells sensitized with allergic serum. Experiments with radioactively-labelled allergic bovine globulin indicated the specificity of the binding of such proteins to rat mast cells. Cross-reaction between reaginic bovine antibody and human IgE was shown by a binding assay involving the uptake of 125I-labelled anti-human IgE globulin by mast cells incubated with bovine passive cutaneous anaphylaxis positive globulin.", "PMID": 965104}
{"id": "pubmed23n0032_6154", "title": "In vitro diagnosis of atopic allergy. V. The value of repeated testing using several batches of the allergen extract.", "content": "The radioallergosorbent test (RAST) has been used for in vitro diagnosis of allergy in a selected group of children with asthma and allergic rhinoconjunctivitis. The results were compared with those of provocation tests and also with the results of skin tests. Where the results of RAST and the provocation tests differed, repeated tests were performed, initially in vitro and secondly in vivo. Selected commercial allergens of good quality were used and, for the most part, the same allergen batches were used in RAST, in the provocation tests and in the skin tests. A 76% correlation between RAST and provocation tests was found on preliminary investigation before divergent results were repeated. This degree of correlation is similar to that obtained previously in unselected patients using RAST as a part of the routine diagnostic. On repeated examination, agreement was obtained in an additional 16% of the cases. The major part of the improvement (13%) was due to conversion of preliminary RAST results from negative to positive in children with positive provocation tests. To a minor extent, the improvement was due to the fact that in, some children with positive RAST, negative provocation tests became positive when repeated. Finally, in one case an originally positive RAST in a child with a negative provocation test was found to be negative on re-examination. Comparing the final revised results of RAST and provocation tests there were no cases where a negative provocation test was combined with a positive RAST. Nearly all the children with positive provocation tests in spite of a negative RAST were shown to have low degree allergy. Although circulating reaginic antibodies are not always present, it seems that, on repeated investigations, the great majority of children with asthma and hay fever can be shown to have such antibodies even though the serum concentration may be low or very low. In doubtful cases repeated RAST examinations may be of value.", "contents": "In vitro diagnosis of atopic allergy. V. The value of repeated testing using several batches of the allergen extract. The radioallergosorbent test (RAST) has been used for in vitro diagnosis of allergy in a selected group of children with asthma and allergic rhinoconjunctivitis. The results were compared with those of provocation tests and also with the results of skin tests. Where the results of RAST and the provocation tests differed, repeated tests were performed, initially in vitro and secondly in vivo. Selected commercial allergens of good quality were used and, for the most part, the same allergen batches were used in RAST, in the provocation tests and in the skin tests. A 76% correlation between RAST and provocation tests was found on preliminary investigation before divergent results were repeated. This degree of correlation is similar to that obtained previously in unselected patients using RAST as a part of the routine diagnostic. On repeated examination, agreement was obtained in an additional 16% of the cases. The major part of the improvement (13%) was due to conversion of preliminary RAST results from negative to positive in children with positive provocation tests. To a minor extent, the improvement was due to the fact that in, some children with positive RAST, negative provocation tests became positive when repeated. Finally, in one case an originally positive RAST in a child with a negative provocation test was found to be negative on re-examination. Comparing the final revised results of RAST and provocation tests there were no cases where a negative provocation test was combined with a positive RAST. Nearly all the children with positive provocation tests in spite of a negative RAST were shown to have low degree allergy. Although circulating reaginic antibodies are not always present, it seems that, on repeated investigations, the great majority of children with asthma and hay fever can be shown to have such antibodies even though the serum concentration may be low or very low. In doubtful cases repeated RAST examinations may be of value.", "PMID": 965105}
{"id": "pubmed23n0032_6155", "title": "Passive cutaneous anaphylaxis in the domestic fowl.", "content": "Passive cutaneous anaphylaxis (PCA) has been demonstrated in young and adult domestic fowl using antisera obtained from actively sensitized chickens on the day of their maximal susceptibility to systemic anaphylaxis. Young chickens are more sensitive to histamine and PCA reaction than adults. The PCA reaction was inhibited by the H1-histamine receptor blocker, mepyramine. In the PCA reaction of 2 h sensitization period, the main exudating cells were heterophils, mononuclear cells (lymphocytes, monocytes and macrophages) and some basophils. This study suggests a possible role for circulating basophils in the mediation of early acute inflammatory reaction of immunological origin.", "contents": "Passive cutaneous anaphylaxis in the domestic fowl. Passive cutaneous anaphylaxis (PCA) has been demonstrated in young and adult domestic fowl using antisera obtained from actively sensitized chickens on the day of their maximal susceptibility to systemic anaphylaxis. Young chickens are more sensitive to histamine and PCA reaction than adults. The PCA reaction was inhibited by the H1-histamine receptor blocker, mepyramine. In the PCA reaction of 2 h sensitization period, the main exudating cells were heterophils, mononuclear cells (lymphocytes, monocytes and macrophages) and some basophils. This study suggests a possible role for circulating basophils in the mediation of early acute inflammatory reaction of immunological origin.", "PMID": 965107}
{"id": "pubmed23n0032_6156", "title": "Activation and consumption of Hageman factor in the anaphylactic shock of the rat.", "content": "In the early stages of anaphylactic shock of rats pretreated with Bordetella pertussis vaccine, a prompt and parallel activation of the factor XIIa-dependent intrinsic coagulation, kinin generation, and fibrinolytic acticity was observed. The coagulation studies, the similarity of anaphylactic results with those produced by a single injection of ellagic acid, and the effective inhibition of the anaphylactic and the ellagic acid-induced activation of these pathways by lysozyme all suggest that factor XII itself becomes activated in rat anaphylaxis. As the reaction proceeded, considerable anticoagulant activities emerged, but the bradykinin and the plasminogen activator levels even further increased. During the first 10 min of anaphylactic shock, factor XII was partly consumed and this was prevented by epsilon-aminocaproic acid infusion. The results show that in pathological conditions such as anaphylaxis there is an intimate in vivo interaction among the three factor XIIa-dependent pathways.", "contents": "Activation and consumption of Hageman factor in the anaphylactic shock of the rat. In the early stages of anaphylactic shock of rats pretreated with Bordetella pertussis vaccine, a prompt and parallel activation of the factor XIIa-dependent intrinsic coagulation, kinin generation, and fibrinolytic acticity was observed. The coagulation studies, the similarity of anaphylactic results with those produced by a single injection of ellagic acid, and the effective inhibition of the anaphylactic and the ellagic acid-induced activation of these pathways by lysozyme all suggest that factor XII itself becomes activated in rat anaphylaxis. As the reaction proceeded, considerable anticoagulant activities emerged, but the bradykinin and the plasminogen activator levels even further increased. During the first 10 min of anaphylactic shock, factor XII was partly consumed and this was prevented by epsilon-aminocaproic acid infusion. The results show that in pathological conditions such as anaphylaxis there is an intimate in vivo interaction among the three factor XIIa-dependent pathways.", "PMID": 965106}
{"id": "pubmed23n0032_6157", "title": "Phytohaemagglutinin (PHA) skin test in predicting prognosis in cancer patients.", "content": "The response to intradermal injection of 1 mug of purified PHA was found to correlate with survival of patients with advanced solid tumours treated by chemotherapy, with responders surviving longer than non-responders. In patients with prolonged survival, the reaction was enhanced. Negative intradermal response to PHA was found to be less common in cancer patients than negative responses to tuberculin or streptokinase, and was more easily reversed by successful chemotherapy.", "contents": "Phytohaemagglutinin (PHA) skin test in predicting prognosis in cancer patients. The response to intradermal injection of 1 mug of purified PHA was found to correlate with survival of patients with advanced solid tumours treated by chemotherapy, with responders surviving longer than non-responders. In patients with prolonged survival, the reaction was enhanced. Negative intradermal response to PHA was found to be less common in cancer patients than negative responses to tuberculin or streptokinase, and was more easily reversed by successful chemotherapy.", "PMID": 965108}
{"id": "pubmed23n0032_6158", "title": "Passive cutaneous anaphylaxis in guinea pigs elicited by gastric absorption of dextran induced by acetylsalicylic acid.", "content": "Passive cutaneous anaphylaxis (PCA) was elicited in guinea pigs sensitized with rabbit antidextran by the absorption of dextran macromolecules from the stomach induced by intragastric acetylsalicylic acid. The gastric contents had a pH sufficiently low to maintain the acid mainly in the unionized form since it is the latter which alters gastric permeability. The acid concentration required to induce PCA was below that which caused mucosal cell loss or bleeding. The maximal molecular weight of the absorbed dextran was approximately 25,000. Dextran was chosen as antigen because of its well-characterized physical and immunological properties. It is suggested that ingestion of acetylsalicylic acid may contribute to sensitization and allergic reactions to antigenic food materials by facilitating their absorption from the stomach.", "contents": "Passive cutaneous anaphylaxis in guinea pigs elicited by gastric absorption of dextran induced by acetylsalicylic acid. Passive cutaneous anaphylaxis (PCA) was elicited in guinea pigs sensitized with rabbit antidextran by the absorption of dextran macromolecules from the stomach induced by intragastric acetylsalicylic acid. The gastric contents had a pH sufficiently low to maintain the acid mainly in the unionized form since it is the latter which alters gastric permeability. The acid concentration required to induce PCA was below that which caused mucosal cell loss or bleeding. The maximal molecular weight of the absorbed dextran was approximately 25,000. Dextran was chosen as antigen because of its well-characterized physical and immunological properties. It is suggested that ingestion of acetylsalicylic acid may contribute to sensitization and allergic reactions to antigenic food materials by facilitating their absorption from the stomach.", "PMID": 965109}
{"id": "pubmed23n0032_6159", "title": "Sugar polymers in rats and man.", "content": "Intracutaneous injections of a glucan contaminant of invert sugar solutions and crude cane sugar into human skin produced a localised wheal and erythema reaction. The glucan was also active on intradermal injection into both dextran-sensitive and dextran-resistant rats.", "contents": "Sugar polymers in rats and man. Intracutaneous injections of a glucan contaminant of invert sugar solutions and crude cane sugar into human skin produced a localised wheal and erythema reaction. The glucan was also active on intradermal injection into both dextran-sensitive and dextran-resistant rats.", "PMID": 965110}
{"id": "pubmed23n0032_6160", "title": "Computerised assessment of cardiac pacemaker performance.", "content": "The design and use of a PDP 11/40 based automated on-line cardiac pacemaker assessment system is described. One program has been developed for testing pacemakers on the bench and another for implanted pacemaker tests. Either fixed rate or demand pacemakers can be tested. The on-line system is easy and fast to use and is also highly accurate. Parameters extracted are pulse width, rate and pulse energy. In addition, a graphical representation of the patient's ECG and the pacemaker pulse is obtained on a computer graphics terminal.", "contents": "Computerised assessment of cardiac pacemaker performance. The design and use of a PDP 11/40 based automated on-line cardiac pacemaker assessment system is described. One program has been developed for testing pacemakers on the bench and another for implanted pacemaker tests. Either fixed rate or demand pacemakers can be tested. The on-line system is easy and fast to use and is also highly accurate. Parameters extracted are pulse width, rate and pulse energy. In addition, a graphical representation of the patient's ECG and the pacemaker pulse is obtained on a computer graphics terminal.", "PMID": 965115}
{"id": "pubmed23n0032_6161", "title": "Analysis of the human respiratory system in hypoxic and anoxic hypoxia by analogue computer simulation.", "content": "The goal of this work is to synthesise a model of an adaptive system of respiration control in man, which could be used for the prognosis of human performance under extreme conditions. The model was partly based on 428 experimental results from hypoxic and anoxic hypoxia. Simulation of the oxygen regulatory circuit was realised on the NADAC 100 and MEDA T 80 analogue computers with satisfactory results. Equations constituting a new version of the model are presented in some detail, together with the results of the simulation of ventilatory mechanics.", "contents": "Analysis of the human respiratory system in hypoxic and anoxic hypoxia by analogue computer simulation. The goal of this work is to synthesise a model of an adaptive system of respiration control in man, which could be used for the prognosis of human performance under extreme conditions. The model was partly based on 428 experimental results from hypoxic and anoxic hypoxia. Simulation of the oxygen regulatory circuit was realised on the NADAC 100 and MEDA T 80 analogue computers with satisfactory results. Equations constituting a new version of the model are presented in some detail, together with the results of the simulation of ventilatory mechanics.", "PMID": 965116}
{"id": "pubmed23n0032_6162", "title": "[Compartmental model of the adsorption of a dye by proteins. The possible role of adsorption in the hepatic excretion of bromosulphophthalein].", "content": "A compartmental model was suggested for the study of the hepatic excretion of bromosulphophthalein (BSP). By using a hypothesis concerning the adsorption of the BSP compound by proteins, one derives from the equations of the model several known experimental results, viz. form of concentration curves that vary in accordance with the strength of the injection, existence of a limiting Tm value, etc. An explicit formula is given for calculating Tm.", "contents": "[Compartmental model of the adsorption of a dye by proteins. The possible role of adsorption in the hepatic excretion of bromosulphophthalein]. A compartmental model was suggested for the study of the hepatic excretion of bromosulphophthalein (BSP). By using a hypothesis concerning the adsorption of the BSP compound by proteins, one derives from the equations of the model several known experimental results, viz. form of concentration curves that vary in accordance with the strength of the injection, existence of a limiting Tm value, etc. An explicit formula is given for calculating Tm.", "PMID": 965117}
{"id": "pubmed23n0032_6163", "title": "Do efferent fibres to hair cells intervene in acoustic stimulus peripheral coding?", "content": "The functional roles suggested for Olivo-Cochlear Bundle (OCB) fibres innervating auditory receptors never received a fully convincing demonstration. Assuming that efferent fibres effects, at the level of hair cells, are inhibitory with regard to single auditory nerve fibre activity, a mathematical model of the peripheral auditory system is proposed in which the presence of the descending pathway is taken into account. Computer simulation of the model accounts for most of the salient features of the patterns of activity of single cochlear nerve fibres. In the light of the model predictions, the influence of OCB fibres on peripheral receptors is assumed to act in the direction of improving the signal-to-noise ratio, as a first step of information processing.", "contents": "Do efferent fibres to hair cells intervene in acoustic stimulus peripheral coding? The functional roles suggested for Olivo-Cochlear Bundle (OCB) fibres innervating auditory receptors never received a fully convincing demonstration. Assuming that efferent fibres effects, at the level of hair cells, are inhibitory with regard to single auditory nerve fibre activity, a mathematical model of the peripheral auditory system is proposed in which the presence of the descending pathway is taken into account. Computer simulation of the model accounts for most of the salient features of the patterns of activity of single cochlear nerve fibres. In the light of the model predictions, the influence of OCB fibres on peripheral receptors is assumed to act in the direction of improving the signal-to-noise ratio, as a first step of information processing.", "PMID": 965118}
{"id": "pubmed23n0032_6164", "title": "Numerical determination of intestinal membrane diffusing constants by a gradient method.", "content": "Optimisation problems arising in the identification of kinetic parameters of intestinal membranes are here considered. The dynamic behaviour of the membrane is described by means of a linear compartmental model. Using optimisation techniques of a gradient type, the intestinal kinetic parameters are identified, minimising a quadratic criterion between experimental data of D-histidine transport and model prediction. Numerical results are reported and their physiological implications discussed. The quantitative assessment of the asymmetry of diffusion constants with respect to diffusion direction seems to be an important result of this work.", "contents": "Numerical determination of intestinal membrane diffusing constants by a gradient method. Optimisation problems arising in the identification of kinetic parameters of intestinal membranes are here considered. The dynamic behaviour of the membrane is described by means of a linear compartmental model. Using optimisation techniques of a gradient type, the intestinal kinetic parameters are identified, minimising a quadratic criterion between experimental data of D-histidine transport and model prediction. Numerical results are reported and their physiological implications discussed. The quantitative assessment of the asymmetry of diffusion constants with respect to diffusion direction seems to be an important result of this work.", "PMID": 965119}
{"id": "pubmed23n0032_6165", "title": "Methadone treatment: program evaluation and dose response relationships.", "content": "Data from a heterogeneous group of 155 clients in a Methadone Treatment Program were analyzed for (A) number of possible methadone administrations since S joined the program, (B) actual number of methadone administrations received, (C) spottiness (A-B), and (D) presence of illicit drugs in urine analysis reports. Data were evaluated for three separate time spans which corresponded with three programmatic changes. Results indicate that initially high blockading doses of methadone are effective in reducing illicit drug use, but as length of time in program increases, the efficacy of low doses of methadone in reducing illicit drug use increases. At least one programmatic change, a more stringent selection procedure, was demonstrated to be highly successful in reducing illicit drug use.", "contents": "Methadone treatment: program evaluation and dose response relationships. Data from a heterogeneous group of 155 clients in a Methadone Treatment Program were analyzed for (A) number of possible methadone administrations since S joined the program, (B) actual number of methadone administrations received, (C) spottiness (A-B), and (D) presence of illicit drugs in urine analysis reports. Data were evaluated for three separate time spans which corresponded with three programmatic changes. Results indicate that initially high blockading doses of methadone are effective in reducing illicit drug use, but as length of time in program increases, the efficacy of low doses of methadone in reducing illicit drug use increases. At least one programmatic change, a more stringent selection procedure, was demonstrated to be highly successful in reducing illicit drug use.", "PMID": 965120}
{"id": "pubmed23n0032_6166", "title": "Attitudes toward alcohol as predictors of self-estimated alcohol consumption in college students.", "content": "The purpose of the present investigation was to examine the relationship between attitudes toward alcohol use and self-estimated drinking behavior. The results indicated that attitudes can reliably predict variations in self-reported drinking behavior. The implication of these findings for treatment and future research is discussed.", "contents": "Attitudes toward alcohol as predictors of self-estimated alcohol consumption in college students. The purpose of the present investigation was to examine the relationship between attitudes toward alcohol use and self-estimated drinking behavior. The results indicated that attitudes can reliably predict variations in self-reported drinking behavior. The implication of these findings for treatment and future research is discussed.", "PMID": 965121}
{"id": "pubmed23n0032_6167", "title": "Cognitive functioning under moderate and low dosage methadone maintenance.", "content": "The Wechsler Adult Intelligence Scale (WAIS) was administered to two groups of patients in the Santa Clara County Methadone Maintenance Program who were receiving low and moderate daily stabilized dosages of methadone hydrochloride. In the two separate statistical analyses performed, there were no significant differences found between subtest scores or verbal, performance, and full-scale scores of the groups measured. These results, combined with observations regarding the similarities of WAIS profiles of the two groups, indicate that cognitive functioning as measured by the WAIS was not differentially affected by the two dosages.", "contents": "Cognitive functioning under moderate and low dosage methadone maintenance. The Wechsler Adult Intelligence Scale (WAIS) was administered to two groups of patients in the Santa Clara County Methadone Maintenance Program who were receiving low and moderate daily stabilized dosages of methadone hydrochloride. In the two separate statistical analyses performed, there were no significant differences found between subtest scores or verbal, performance, and full-scale scores of the groups measured. These results, combined with observations regarding the similarities of WAIS profiles of the two groups, indicate that cognitive functioning as measured by the WAIS was not differentially affected by the two dosages.", "PMID": 965122}
{"id": "pubmed23n0032_6168", "title": "Case-note abstraction: a methodological substudy as cautionary tale.", "content": "A sample of 42 case notes of alcoholic patients were abstracted for 17 items by three different raters. Interrater agreement was generally rather low. Research based on case-note abstraction which does not report on abstraction reliabilities must therefore be viewed with some suspicion. It would be helpful if clinical material could more often be collected in a standardized manner.", "contents": "Case-note abstraction: a methodological substudy as cautionary tale. A sample of 42 case notes of alcoholic patients were abstracted for 17 items by three different raters. Interrater agreement was generally rather low. Research based on case-note abstraction which does not report on abstraction reliabilities must therefore be viewed with some suspicion. It would be helpful if clinical material could more often be collected in a standardized manner.", "PMID": 965123}
{"id": "pubmed23n0032_6169", "title": "The heroin epidemic in San Francisco: estimates of incidence and prevalence.", "content": "Much attention has recently been focused on the question of the extent of heroin use in America. In a worthwhile effort to call public attention to the problem, many estimates of its size have evidenced a tendency toward exaggeration and aggrandizement. This paper presents methods of estimation of the extent of heroin addiction which, when carefully employed, should effectively correct such distorted estimates. Two general types of estimation are employed, incidence and prevalence. Incidence estimates are concerned with new cases of heroin addiction that occur in a specific population within a given amount of time. These estimates are based upon self-report data from addicts regarding date of first addiction. When corrected for the \"lag phase\" (that period of time between onset of first addiction and eventual visit to a treatment program), these data permit accurate retrospective charting of incidence trends. Prevalence estimates focus on all known cases of heroin addiction in a specific population within a given amount of time. Three separate types of prevalence estimates from three separate sources are outlined in this article: estimates based on overdose death data, estimates based on crime statistics, and estimates of \"unknown\" addicts. In outlining these methods, this article describes the fluctuations in heroin addiction in one major American city, San Francisco, California. After analyzing data gathered from a sample of 2,367 addicts contacted over a 3-year period, this study suggests that the incidence of heroin addiction seems to have declined after 1970. Possible factors underlying this apparent decline in heroin addiction are then discussed, including the post-1970 maturation of the \"population at risk,\" the effectiveness of antidrug media messages, the changing drug fashions in the heroin subculture, and the gradual deterioration of the quality and potency of street heroin.", "contents": "The heroin epidemic in San Francisco: estimates of incidence and prevalence. Much attention has recently been focused on the question of the extent of heroin use in America. In a worthwhile effort to call public attention to the problem, many estimates of its size have evidenced a tendency toward exaggeration and aggrandizement. This paper presents methods of estimation of the extent of heroin addiction which, when carefully employed, should effectively correct such distorted estimates. Two general types of estimation are employed, incidence and prevalence. Incidence estimates are concerned with new cases of heroin addiction that occur in a specific population within a given amount of time. These estimates are based upon self-report data from addicts regarding date of first addiction. When corrected for the \"lag phase\" (that period of time between onset of first addiction and eventual visit to a treatment program), these data permit accurate retrospective charting of incidence trends. Prevalence estimates focus on all known cases of heroin addiction in a specific population within a given amount of time. Three separate types of prevalence estimates from three separate sources are outlined in this article: estimates based on overdose death data, estimates based on crime statistics, and estimates of \"unknown\" addicts. In outlining these methods, this article describes the fluctuations in heroin addiction in one major American city, San Francisco, California. After analyzing data gathered from a sample of 2,367 addicts contacted over a 3-year period, this study suggests that the incidence of heroin addiction seems to have declined after 1970. Possible factors underlying this apparent decline in heroin addiction are then discussed, including the post-1970 maturation of the \"population at risk,\" the effectiveness of antidrug media messages, the changing drug fashions in the heroin subculture, and the gradual deterioration of the quality and potency of street heroin.", "PMID": 965124}
{"id": "pubmed23n0032_6170", "title": "Readiness for rehabilitation.", "content": "High dropout rates of addicts in treatment have long been attributed to a lack of motivation or readiness for rehabilitation. Two groups of subjects were compared on several demographic characteristics and several variables isolated as possible predictors of readiness for rehabilitation.", "contents": "Readiness for rehabilitation. High dropout rates of addicts in treatment have long been attributed to a lack of motivation or readiness for rehabilitation. Two groups of subjects were compared on several demographic characteristics and several variables isolated as possible predictors of readiness for rehabilitation.", "PMID": 965125}
{"id": "pubmed23n0032_6171", "title": "The validity of self-recording reports made by drug and alcohol abusers in a residential setting.", "content": "In this study a self-recording procedure was used to clarify the relationship between urges or cravings for drugs (including alcohol) and actual drug (including alcohol) use within a residential setting. There were two purposes of the study: first, to evaluate the predictive validity of the self-recording procedure; and second, to generate empirical data relevant to the concept of craving, which is crucial to many theories of drug and alcohol addiction. The predictive validity of the urge reports was evaluated using a behavioral measure--actual and suspected drug use incidents. These incidents were reported by staff members on all drug and alcohol abusers in residential treatment during a 2-month period. The results indicate that there is no significant tendency for there to be an increased use of drugs on the same days that drug urges are reported, and that drug use incidents are most likely associated with reports of no urges for drugs. Therefore, the predictive validity of the self-recording reports was not established. The data challenge the notion that drug usage is preceeded by or accompanied by (intense, driving) drug cravings at least within this setting.", "contents": "The validity of self-recording reports made by drug and alcohol abusers in a residential setting. In this study a self-recording procedure was used to clarify the relationship between urges or cravings for drugs (including alcohol) and actual drug (including alcohol) use within a residential setting. There were two purposes of the study: first, to evaluate the predictive validity of the self-recording procedure; and second, to generate empirical data relevant to the concept of craving, which is crucial to many theories of drug and alcohol addiction. The predictive validity of the urge reports was evaluated using a behavioral measure--actual and suspected drug use incidents. These incidents were reported by staff members on all drug and alcohol abusers in residential treatment during a 2-month period. The results indicate that there is no significant tendency for there to be an increased use of drugs on the same days that drug urges are reported, and that drug use incidents are most likely associated with reports of no urges for drugs. Therefore, the predictive validity of the self-recording reports was not established. The data challenge the notion that drug usage is preceeded by or accompanied by (intense, driving) drug cravings at least within this setting.", "PMID": 965126}
{"id": "pubmed23n0032_6172", "title": "Interpersonal behavior in a small group setting during the heroin addiction cycle.", "content": "In a study of social interaction during an experimental addiction cycle, male narcotic addicts expressed more hostility after higher doses of heroin. No consistent role variations were observed in dominance, friendliness, or therapeutic-orientation.", "contents": "Interpersonal behavior in a small group setting during the heroin addiction cycle. In a study of social interaction during an experimental addiction cycle, male narcotic addicts expressed more hostility after higher doses of heroin. No consistent role variations were observed in dominance, friendliness, or therapeutic-orientation.", "PMID": 965127}
{"id": "pubmed23n0032_6173", "title": "Psychopathology, craving, and mood during heroin acquisition: an experimental study.", "content": "Six detoxified addict volunteers were allowed to self-administer intravenous heroin on an essentially self-determined schedule. Two periods of heroin acquisition were compared: an unmodified cycle in which patients could become intoxicated and a later cycle in which the effects of heroin were blocked with a narcotic antagonist. In the unblocked condition, patients initially experienced an increase in positive mood, but with chronic administration there was a significant rise in psychopathology and the development of a generalized dysphoric state. Similar changes did not occur when the same patients took heroin while blocked with a narcotic antagonist. Drug craving rose dramatically when \"unblocked\" heroin was available, but gradually fell during methadone detoxification. Following treatment with a narcotic antagonist, the presence of heroin failed to elicit any sustained rise in craving and drug taking was dramatically reduced.", "contents": "Psychopathology, craving, and mood during heroin acquisition: an experimental study. Six detoxified addict volunteers were allowed to self-administer intravenous heroin on an essentially self-determined schedule. Two periods of heroin acquisition were compared: an unmodified cycle in which patients could become intoxicated and a later cycle in which the effects of heroin were blocked with a narcotic antagonist. In the unblocked condition, patients initially experienced an increase in positive mood, but with chronic administration there was a significant rise in psychopathology and the development of a generalized dysphoric state. Similar changes did not occur when the same patients took heroin while blocked with a narcotic antagonist. Drug craving rose dramatically when \"unblocked\" heroin was available, but gradually fell during methadone detoxification. Following treatment with a narcotic antagonist, the presence of heroin failed to elicit any sustained rise in craving and drug taking was dramatically reduced.", "PMID": 965128}
{"id": "pubmed23n0032_6174", "title": "Drug interactions of the components of Optalidon after oral administration.", "content": "An investigation involving seven successive was undertaken on several groups of 10 to 14 volunteers, in order to evaluate any drug interaction between the three active components of Optalidon, namely amidopyrine (A), butalbital (B), and caffeine (C). Each component was investigated after oral administration, alone and in combination either with one of the others (i.e. A+B, B+C, C+A) or with both of the others in Optalidon (A+B+C). The plasma concentration and urinary excretion were recorded for each component as a function of time. For amidopyrine, two metabolites, amino-4-antipyrine and acetamino-4-antipyrine, were also measured in the urine. Based on a pharmacokinetic model, the following conclusions can be drawn: a) There is no change in bioavailability due to the combination of the three components in Optalidon in respect to their single administration. Within each study, there is no significant difference between the elimination rate constants, areas under the plasma concentration/time curve and percentage excreted in urine for the three components administered alone or in any combination with the other components of Optalidon. b) Concerning the absorption half-life, there is no change for amidopyrine. Only caffeine and butalbital show a statistically significant interaction in respect to this parameter and, as a consequence, differences in the time and value of the maximal plasma concentration in Optalidon. However, these differences are scarcely of anyl clinical relevance.", "contents": "Drug interactions of the components of Optalidon after oral administration. An investigation involving seven successive was undertaken on several groups of 10 to 14 volunteers, in order to evaluate any drug interaction between the three active components of Optalidon, namely amidopyrine (A), butalbital (B), and caffeine (C). Each component was investigated after oral administration, alone and in combination either with one of the others (i.e. A+B, B+C, C+A) or with both of the others in Optalidon (A+B+C). The plasma concentration and urinary excretion were recorded for each component as a function of time. For amidopyrine, two metabolites, amino-4-antipyrine and acetamino-4-antipyrine, were also measured in the urine. Based on a pharmacokinetic model, the following conclusions can be drawn: a) There is no change in bioavailability due to the combination of the three components in Optalidon in respect to their single administration. Within each study, there is no significant difference between the elimination rate constants, areas under the plasma concentration/time curve and percentage excreted in urine for the three components administered alone or in any combination with the other components of Optalidon. b) Concerning the absorption half-life, there is no change for amidopyrine. Only caffeine and butalbital show a statistically significant interaction in respect to this parameter and, as a consequence, differences in the time and value of the maximal plasma concentration in Optalidon. However, these differences are scarcely of anyl clinical relevance.", "PMID": 965130}
{"id": "pubmed23n0032_6175", "title": "A contribution to the pharmacokinetics of bencyclane (Fludilat) in man.", "content": "The quantitative determination of bencyclane from the biological material was carried out with the aid of a combined microchemical method (thin-layer chromatography and measurement of fluorescence) using NBD chloride. The original method [J. Reisch, Z. Analyt. Chemie, 247 (1969) 56; J. Monforte, Clinical Chemistry 18 (1972) 1329; R.S. Fager, Anal. Biochemistry, 53 (1973) 290, etc.] was so modified as to enable attainment of optimal results in respect of sensitivity and accuracy in the determination of bencyclane. The sensitivity of this modified method is 0.1 mug/ml plasma. Volunteer subjects and patients received under standard conditions 2 coated tablets Fludilat (i.e. 200 mg bencyclane hydrogen fumarate) orally as a single dose or repeated 3 times daily over 5 days, or 4 ampoules (= 200 mg) in a single intravenous injection. After a single oral administration, maximum plasma concentrations of approximately 2 mug/ml were attained in about 2 hours. The elimination half-life was about 360-480 min. The appearance of a second peak after about 6-7 hours indicates involvement of several compartments. On intravenous administration, maximum plasma concentrations of above 2 mug/ml were attained. A second peak in the late phase of the elimination was also detected here. The repeated oral administration led to maximum plasma concentrations of above 3 mug/ml without there being any indication of accumulation. Protein binding of about 30% was determined with the aid of the equilibrium dialysis method. A parallel \"in vitro\" study with 14C-bencyclane (U.R. Kleeberg, 1973, unpublished) showed an approx. 40% protein binding, an approx. 30% erythrocyte binding, and an approx. 10% thrombocyte binding. About 20% bencyclane remain free.", "contents": "A contribution to the pharmacokinetics of bencyclane (Fludilat) in man. The quantitative determination of bencyclane from the biological material was carried out with the aid of a combined microchemical method (thin-layer chromatography and measurement of fluorescence) using NBD chloride. The original method [J. Reisch, Z. Analyt. Chemie, 247 (1969) 56; J. Monforte, Clinical Chemistry 18 (1972) 1329; R.S. Fager, Anal. Biochemistry, 53 (1973) 290, etc.] was so modified as to enable attainment of optimal results in respect of sensitivity and accuracy in the determination of bencyclane. The sensitivity of this modified method is 0.1 mug/ml plasma. Volunteer subjects and patients received under standard conditions 2 coated tablets Fludilat (i.e. 200 mg bencyclane hydrogen fumarate) orally as a single dose or repeated 3 times daily over 5 days, or 4 ampoules (= 200 mg) in a single intravenous injection. After a single oral administration, maximum plasma concentrations of approximately 2 mug/ml were attained in about 2 hours. The elimination half-life was about 360-480 min. The appearance of a second peak after about 6-7 hours indicates involvement of several compartments. On intravenous administration, maximum plasma concentrations of above 2 mug/ml were attained. A second peak in the late phase of the elimination was also detected here. The repeated oral administration led to maximum plasma concentrations of above 3 mug/ml without there being any indication of accumulation. Protein binding of about 30% was determined with the aid of the equilibrium dialysis method. A parallel \"in vitro\" study with 14C-bencyclane (U.R. Kleeberg, 1973, unpublished) showed an approx. 40% protein binding, an approx. 30% erythrocyte binding, and an approx. 10% thrombocyte binding. About 20% bencyclane remain free.", "PMID": 965131}
{"id": "pubmed23n0032_6176", "title": "[Elimination kinetics of phenprocoumon (Marcumar) in liver cirrhosis and after premedication with phenobarbital].", "content": "In this paper are presented the results of a study on the elimination kinetics of phenprocoumon in patients concomitantly treated with phenprocoumon and phenobarbital as well as in patients with bioptically proved hepatic cirrhosis. The pre-treatment of the patients with phenobarbital (3 X 100 mg), 4 days before phenprocoumon was started, resulted in an accelerated elimination of phenprocoumon. The biological half-life of phenprocoumon was reduced from 150 to 70 hrs when compared to normal controls, and the elimination constant was found to increase to 9,96 X 10(-3). Phenobarbital at plasma concentrations achieved by usual therapeutical dosage schedules of the drug does not interfere with the protein binding of phenprocoumon as could be shown by equilibrium dialysis. Phenobarbital, therefore, does not accelerate the elimination of phenprocoumon. The inducing effect of phenobarbital on the drug metabolizing enzyme system in the liver results in more rapid biotransformation of phenprocoumon. In patients with bioptically proved hepatic cirrhosis, the elimination kinetics did not differ from the results obtained in normal controls.", "contents": "[Elimination kinetics of phenprocoumon (Marcumar) in liver cirrhosis and after premedication with phenobarbital]. In this paper are presented the results of a study on the elimination kinetics of phenprocoumon in patients concomitantly treated with phenprocoumon and phenobarbital as well as in patients with bioptically proved hepatic cirrhosis. The pre-treatment of the patients with phenobarbital (3 X 100 mg), 4 days before phenprocoumon was started, resulted in an accelerated elimination of phenprocoumon. The biological half-life of phenprocoumon was reduced from 150 to 70 hrs when compared to normal controls, and the elimination constant was found to increase to 9,96 X 10(-3). Phenobarbital at plasma concentrations achieved by usual therapeutical dosage schedules of the drug does not interfere with the protein binding of phenprocoumon as could be shown by equilibrium dialysis. Phenobarbital, therefore, does not accelerate the elimination of phenprocoumon. The inducing effect of phenobarbital on the drug metabolizing enzyme system in the liver results in more rapid biotransformation of phenprocoumon. In patients with bioptically proved hepatic cirrhosis, the elimination kinetics did not differ from the results obtained in normal controls.", "PMID": 965132}
{"id": "pubmed23n0032_6177", "title": "Hemodynamic effects of thiothixene and chlorpromazine in schizophrenic patients at rest and during exercise.", "content": "The hemodynamic effects and plasma levels of noradrenaline were studied in schizophrenic patients at rest and during exercise after long-term treatment with chlorpromazine (150-600 mg daily) and thiothixene (60-80 mg daily). The results are compared with those from previous studies in untreated patients and patients receiving very large doses of chlorpromazine. The effects of thiothixene on the different hemodynamic variables were very moderate, and the observed differences between this group and the control group may be due to the different patient materials. In the two groups of patients receiving chlorpromazine, the heart rate at rest and durng exercise tended to be higher than in the control group. There was also a tendency towards a lower stroke volume after this drug and thiothixene during exercise. The noradrenaline levels in plasma were highest after the high dose of chlorpromazine both at rest and during exercise, while they were lower after the moderate chlorpromazine dose. After thiothixene, the values were between those of the group on the low chlorpromazine dose and those of the control group.", "contents": "Hemodynamic effects of thiothixene and chlorpromazine in schizophrenic patients at rest and during exercise. The hemodynamic effects and plasma levels of noradrenaline were studied in schizophrenic patients at rest and during exercise after long-term treatment with chlorpromazine (150-600 mg daily) and thiothixene (60-80 mg daily). The results are compared with those from previous studies in untreated patients and patients receiving very large doses of chlorpromazine. The effects of thiothixene on the different hemodynamic variables were very moderate, and the observed differences between this group and the control group may be due to the different patient materials. In the two groups of patients receiving chlorpromazine, the heart rate at rest and durng exercise tended to be higher than in the control group. There was also a tendency towards a lower stroke volume after this drug and thiothixene during exercise. The noradrenaline levels in plasma were highest after the high dose of chlorpromazine both at rest and during exercise, while they were lower after the moderate chlorpromazine dose. After thiothixene, the values were between those of the group on the low chlorpromazine dose and those of the control group.", "PMID": 965133}
{"id": "pubmed23n0032_6178", "title": "Effects of xipamide and triamterene - alone or in combination - on electrolyte excretion in the rat.", "content": "The effects of X and T in different doses alone or in combination on renal sodium and potassium excretion were examined in conscious rats. In acute experiments, the combination of both diuretics produced an approximately additive effect on sodium excretion. Addition of T to X in different dose relations (X:T = 1:0.5, 1:1 or 1:1.5) prevented the X-induced potassium loss. These effects were more pronounced at the highest dose of T; the difference to the other combinations, however, was small. In chronic experiments, when the diuretics were administered daily for 13 days, both combinations (X:T = 1:1 and X:T = 1:1.5) resulted in a nearly equilibrated potassium balance at the end of this period. In shorter intervals, however, sometimes considerable oscillations in potassium excretion occured.", "contents": "Effects of xipamide and triamterene - alone or in combination - on electrolyte excretion in the rat. The effects of X and T in different doses alone or in combination on renal sodium and potassium excretion were examined in conscious rats. In acute experiments, the combination of both diuretics produced an approximately additive effect on sodium excretion. Addition of T to X in different dose relations (X:T = 1:0.5, 1:1 or 1:1.5) prevented the X-induced potassium loss. These effects were more pronounced at the highest dose of T; the difference to the other combinations, however, was small. In chronic experiments, when the diuretics were administered daily for 13 days, both combinations (X:T = 1:1 and X:T = 1:1.5) resulted in a nearly equilibrated potassium balance at the end of this period. In shorter intervals, however, sometimes considerable oscillations in potassium excretion occured.", "PMID": 965134}
{"id": "pubmed23n0032_6179", "title": "Contact allergy to corticosteroids.", "content": "Contact dermatitis to topical corticosteroids does occur, although infrequently. Patch testing to the available steroids is recommended for patients with long-standing dermatoses which have responded poorly to topical steroids.", "contents": "Contact allergy to corticosteroids. Contact dermatitis to topical corticosteroids does occur, although infrequently. Patch testing to the available steroids is recommended for patients with long-standing dermatoses which have responded poorly to topical steroids.", "PMID": 965139}
{"id": "pubmed23n0032_6180", "title": "The haem-accessibility in leghaemoglobin of Lupinus luteus as observed by proton magnetic relaxation.", "content": "Using the solvent-protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our \"stereo-chemical p.m.r. titration\" method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHZ. Taking into account the measured value of tauc = (7.7 +/- 0.5 x 10(-10) s the iron-to-proton inter-spin distances have been calculated. The significance of these distances as well as the electronic g-factor anisotrophy for elucidation of fine structural details of the haem-environment are discussed.", "contents": "The haem-accessibility in leghaemoglobin of Lupinus luteus as observed by proton magnetic relaxation. Using the solvent-protons' longitudinal magnetic relaxation rates (p.m.r.) for Lupinus luteus leghaemoglobin derivatives the accessibility of the haem has been evaluated by our \"stereo-chemical p.m.r. titration\" method with nonexchangeable protons of aliphatic lower alcohols in otherwise deuterated solutions. The haem in leghaemoglobin is more accessible and its protein environment more flexible compared with vertebrate haemoglobins. The correlation time in aquometleghaemglobin aqueous solution has been determined by measuring the frequency dispersion of the p.m.r. rates between 6.1 and 93 MHZ. Taking into account the measured value of tauc = (7.7 +/- 0.5 x 10(-10) s the iron-to-proton inter-spin distances have been calculated. The significance of these distances as well as the electronic g-factor anisotrophy for elucidation of fine structural details of the haem-environment are discussed.", "PMID": 965150}
{"id": "pubmed23n0032_6181", "title": "A square pyramidal model for ribonuclease action.", "content": "A mechanism incorporating a square pyramidal model (SP) is presented for the action of bovine pancreatic ribonuclease. Its formulation is based on structural principles governing pentacoordinate behavior. The model is compared with a previous trigonal bipyramidal (TP) representation with regard to the geometry of the active site and enzyme constraints. Of two variants of the SP model, an adjacent (cis displacement) and in-line (trans displacement) process, the in-line mechanism, as with the TP model, fits existing model studies. Consideration of the energetics of the SP vs. the TP model leads to an estimated energy difference of about 1-2 kcal/mol. This suggests that the preferred model may be intermediate in geometry between the two idealized representations for the enzymatic hydrolysis. Comparisons are made showing that pseudorotation is an unlikely process in either model.", "contents": "A square pyramidal model for ribonuclease action. A mechanism incorporating a square pyramidal model (SP) is presented for the action of bovine pancreatic ribonuclease. Its formulation is based on structural principles governing pentacoordinate behavior. The model is compared with a previous trigonal bipyramidal (TP) representation with regard to the geometry of the active site and enzyme constraints. Of two variants of the SP model, an adjacent (cis displacement) and in-line (trans displacement) process, the in-line mechanism, as with the TP model, fits existing model studies. Consideration of the energetics of the SP vs. the TP model leads to an estimated energy difference of about 1-2 kcal/mol. This suggests that the preferred model may be intermediate in geometry between the two idealized representations for the enzymatic hydrolysis. Comparisons are made showing that pseudorotation is an unlikely process in either model.", "PMID": 965152}
{"id": "pubmed23n0032_6182", "title": "Retro analogs related to oxytocin.", "content": "[D-alle3]-retro-D-deaminotocinamide (I), retro-L-deaminotocinamide (III) and their respective N-formyl derivatives (II and IV) were synthesized by the stepwise active ester method: deaminotocinamide was prepared by the solid-phase method. The retro-analogs of deaminotocinamide, tested at concentrations up to 10)-5)M, were found to be without activity as agonists or antagonists in the oxytocic assay. At 10(-4)M,[D-alle]-retro-D-deaminotocinamide is a weak competitive inhibitor of oxytocin. [D-alle3, Gly7]-retro-D-deaminooxytocin (V) was synthesized either by the active ester method or by a fragment condensation method employing the retro-D-RING, [D-alle3]-retro-D-deaminotocinamide, and D-tail, Boc-Gly-D-Leu-Gly, as the fragments...", "contents": "Retro analogs related to oxytocin. [D-alle3]-retro-D-deaminotocinamide (I), retro-L-deaminotocinamide (III) and their respective N-formyl derivatives (II and IV) were synthesized by the stepwise active ester method: deaminotocinamide was prepared by the solid-phase method. The retro-analogs of deaminotocinamide, tested at concentrations up to 10)-5)M, were found to be without activity as agonists or antagonists in the oxytocic assay. At 10(-4)M,[D-alle]-retro-D-deaminotocinamide is a weak competitive inhibitor of oxytocin. [D-alle3, Gly7]-retro-D-deaminooxytocin (V) was synthesized either by the active ester method or by a fragment condensation method employing the retro-D-RING, [D-alle3]-retro-D-deaminotocinamide, and D-tail, Boc-Gly-D-Leu-Gly, as the fragments...", "PMID": 965155}
{"id": "pubmed23n0032_6183", "title": "Chemical study of the topography of porcine lutropin (LH) using dinitrofluorobenzene and dansyl chloride.", "content": "Treatment of porcine lutropin beta-subunit by increasing amounts of dansyl chloride shows that only one fluorescent group can be bound to the free subunit, namely on tyrosine beta-37. This modification prevents reassociation with native chi-subunit. In contrast to dansyl chloride, dinitrofluorobenzene reacts preferentially with tyrosine beta-59. This substitution does not interfere with the reassociation with the native chi-subunit. By using equimolar ratio of dansyl chloride and porcine lutropin chi-subunit it is possible to modify this subunit on a single site, which is found to be a tyrosine residue. The monodansylated X-subunit is still able to recombine with native B-subunit but its fluorescence is found to be markedly quenched upon binding. In addition, the O-dansyl-tyrosyl fluorescence quenching by potassium iodide is more effective on the recombined dimer than on the free chi-subunit. Both reconstituted dimers (native xhi chi dinitrophenylated beta and dansylated chi chi native beta) are without biological activity. It is not clear whether the substituted phenolic groups are essential or whether the added groups prevent the chi beta dimer from taking the active conformation. This alternative is discussed in the light of recent data from this laboratory and others.", "contents": "Chemical study of the topography of porcine lutropin (LH) using dinitrofluorobenzene and dansyl chloride. Treatment of porcine lutropin beta-subunit by increasing amounts of dansyl chloride shows that only one fluorescent group can be bound to the free subunit, namely on tyrosine beta-37. This modification prevents reassociation with native chi-subunit. In contrast to dansyl chloride, dinitrofluorobenzene reacts preferentially with tyrosine beta-59. This substitution does not interfere with the reassociation with the native chi-subunit. By using equimolar ratio of dansyl chloride and porcine lutropin chi-subunit it is possible to modify this subunit on a single site, which is found to be a tyrosine residue. The monodansylated X-subunit is still able to recombine with native B-subunit but its fluorescence is found to be markedly quenched upon binding. In addition, the O-dansyl-tyrosyl fluorescence quenching by potassium iodide is more effective on the recombined dimer than on the free chi-subunit. Both reconstituted dimers (native xhi chi dinitrophenylated beta and dansylated chi chi native beta) are without biological activity. It is not clear whether the substituted phenolic groups are essential or whether the added groups prevent the chi beta dimer from taking the active conformation. This alternative is discussed in the light of recent data from this laboratory and others.", "PMID": 965156}
{"id": "pubmed23n0032_6184", "title": "The phosphopyridoxyl peptide from the mitochondrial aspartate aminotransferase of beef kidney.", "content": "The sequence around the coenzyme-binding lysine of mitochondrial aspartate amino-transferase from beef kidney was determined. The holoenzyme was treated with NaB3H4 and digested with thermolysin; the labelled peptide was isolated and its sequence proved to be identical with sequences around the coenzyme site from the same isoenzyme of different organs and animals (pig heart and sheep liver). The sequences of the phosphopyridoxyl peptides of the mitochondrial aspartate aminotransferases appear to be closely related to the corresponding peptides from the cytoplasmic isoenzymes.", "contents": "The phosphopyridoxyl peptide from the mitochondrial aspartate aminotransferase of beef kidney. The sequence around the coenzyme-binding lysine of mitochondrial aspartate amino-transferase from beef kidney was determined. The holoenzyme was treated with NaB3H4 and digested with thermolysin; the labelled peptide was isolated and its sequence proved to be identical with sequences around the coenzyme site from the same isoenzyme of different organs and animals (pig heart and sheep liver). The sequences of the phosphopyridoxyl peptides of the mitochondrial aspartate aminotransferases appear to be closely related to the corresponding peptides from the cytoplasmic isoenzymes.", "PMID": 965157}
{"id": "pubmed23n0032_6185", "title": "Synthesis of peptides and derivatives of 3- and 4- hydroxyproline.", "content": "Synthesis and properties are reported for a number of peptides and related derivatives of 3-hydroxy-L-proline and 4-hydroxyl-L-proline. These were made for several different purposes, namely, sythesis of N-acetyl-O-tosyl-4-hydroxy-L-proline amide as a model for chemical reduction of peptide-bound hydroxyproline, synthesis of tripeptides with the sequence glycyl-4-hydroxy-L-hydroxy-L-prolyl-X, synthesis of the naturally occurring sequence, glycyl-3-hydroxy-L-prolyl-4-hydroxy-L-proline, and monomers for polymerization to yield the sequence -glycyl-prolyl-4-hydroxy-L-prolyl-.", "contents": "Synthesis of peptides and derivatives of 3- and 4- hydroxyproline. Synthesis and properties are reported for a number of peptides and related derivatives of 3-hydroxy-L-proline and 4-hydroxyl-L-proline. These were made for several different purposes, namely, sythesis of N-acetyl-O-tosyl-4-hydroxy-L-proline amide as a model for chemical reduction of peptide-bound hydroxyproline, synthesis of tripeptides with the sequence glycyl-4-hydroxy-L-hydroxy-L-prolyl-X, synthesis of the naturally occurring sequence, glycyl-3-hydroxy-L-prolyl-4-hydroxy-L-proline, and monomers for polymerization to yield the sequence -glycyl-prolyl-4-hydroxy-L-prolyl-.", "PMID": 965158}
{"id": "pubmed23n0032_6186", "title": "Late results following substitution of colon for the stomach, esophagus, urinary bladder and vagina.", "content": "The transverse colon was substituted for the stomach in four patients after total gastrectomy for cancer. Three of them are alive after 21 to 23 years. They are in good condition and fully active. In six patients the esophagus was resected and the colon was interposed transthoracically. Two patients survive 13 to 15 years later. Carcinoma of the urinary bladder was resected three times and colon substituted. In a case of total vaginal aplasia, sigmoid colon was substituted for the vagina. The patient is alive after 17 years and has a normal sexual life. Sixteen patients have had colon transplantations to replace various organs. Seven of them are alive. The average time of survival thus far is 19 years.", "contents": "Late results following substitution of colon for the stomach, esophagus, urinary bladder and vagina. The transverse colon was substituted for the stomach in four patients after total gastrectomy for cancer. Three of them are alive after 21 to 23 years. They are in good condition and fully active. In six patients the esophagus was resected and the colon was interposed transthoracically. Two patients survive 13 to 15 years later. Carcinoma of the urinary bladder was resected three times and colon substituted. In a case of total vaginal aplasia, sigmoid colon was substituted for the vagina. The patient is alive after 17 years and has a normal sexual life. Sixteen patients have had colon transplantations to replace various organs. Seven of them are alive. The average time of survival thus far is 19 years.", "PMID": 965171}
{"id": "pubmed23n0032_6187", "title": "A ten-year experience in the evaluation and management of patients with stress urinary incontinence.", "content": "The surgical procedures used, the complications encountered and the results obtained in 549 patients with urinary stress incontinence with or without prolapse and 50 patients with prolapse without urinary stress incontinence are presented. Incontinence was cured in 347 patients, improved in 126, unchanged in 66 and worsened in ten. Seven patients operated on for uterine prolapse developed urinary incontinence after surgery. The overall recurrence of SUI was 12.75%. The introduction of suprapubic bladder drainage has practically eliminated postoperative urinary tract infections and reduced the length of hospitalization from 9.1 to 7.2 days. My experience in 214 patients with suprabpubic drainage demonstratedthe superiority of the Ansari method over the cystocath. The addition of Cantor's bladder neck plication improved the results (cured plus improved) from 80% to 100% in the Marshall-Marchetti-Krantz operation and from 81% to 86% when the Marshall-Marchetti-Krantz operation was associated with an abdominal hysterectomy.", "contents": "A ten-year experience in the evaluation and management of patients with stress urinary incontinence. The surgical procedures used, the complications encountered and the results obtained in 549 patients with urinary stress incontinence with or without prolapse and 50 patients with prolapse without urinary stress incontinence are presented. Incontinence was cured in 347 patients, improved in 126, unchanged in 66 and worsened in ten. Seven patients operated on for uterine prolapse developed urinary incontinence after surgery. The overall recurrence of SUI was 12.75%. The introduction of suprapubic bladder drainage has practically eliminated postoperative urinary tract infections and reduced the length of hospitalization from 9.1 to 7.2 days. My experience in 214 patients with suprabpubic drainage demonstratedthe superiority of the Ansari method over the cystocath. The addition of Cantor's bladder neck plication improved the results (cured plus improved) from 80% to 100% in the Marshall-Marchetti-Krantz operation and from 81% to 86% when the Marshall-Marchetti-Krantz operation was associated with an abdominal hysterectomy.", "PMID": 965172}
{"id": "pubmed23n0032_6188", "title": "Polycystography in the evaluation of carcinoma of the urinary bladder.", "content": "Polycystography is a safe, simple, useful, radiological procedure to assess the degree of infiltration of bladder tumors. It broadly classifies bladder tumors into two groups: infiltrating and noninfiltrating. This information is important to plan the treatment. In 96% of the cases, the radiological findings correlated well with other methods of assessing the degree of infiltration. The standard technic, modifications, advantages and disadvantages of this procedure are discussed.", "contents": "Polycystography in the evaluation of carcinoma of the urinary bladder. Polycystography is a safe, simple, useful, radiological procedure to assess the degree of infiltration of bladder tumors. It broadly classifies bladder tumors into two groups: infiltrating and noninfiltrating. This information is important to plan the treatment. In 96% of the cases, the radiological findings correlated well with other methods of assessing the degree of infiltration. The standard technic, modifications, advantages and disadvantages of this procedure are discussed.", "PMID": 965174}
{"id": "pubmed23n0032_6189", "title": "Treatment of lithiasis by the forced injection of liquid and ureteral catheterization by the translumbar route.", "content": "We have used translumbar intrapelvic injection of liquid and intraureteral sliding of a catheter to push ureteral calculi and obtain their elimination through the bladder in 32 patients. We have described the technic and the results obtained. We had more success with the intraureteral catheter technic. No complications were observed in our cases. Ureteral physiology in relation to the favorable results obtained is discussed.", "contents": "Treatment of lithiasis by the forced injection of liquid and ureteral catheterization by the translumbar route. We have used translumbar intrapelvic injection of liquid and intraureteral sliding of a catheter to push ureteral calculi and obtain their elimination through the bladder in 32 patients. We have described the technic and the results obtained. We had more success with the intraureteral catheter technic. No complications were observed in our cases. Ureteral physiology in relation to the favorable results obtained is discussed.", "PMID": 965175}
{"id": "pubmed23n0032_6190", "title": "Effect of cataract on intraocular pressure.", "content": "Cases of immature and mature cataract were selected to study normal values in relation to hypermature cataract. We observed that cases of hypermature cataract had a high intraocular tension which was associated with a high protein level and increase in the number of macrophages in the aqueous. We concluded that a phacolytic mechanism is responsible for a secondary rise of intraocular tension in cases of hypermature cataract.", "contents": "Effect of cataract on intraocular pressure. Cases of immature and mature cataract were selected to study normal values in relation to hypermature cataract. We observed that cases of hypermature cataract had a high intraocular tension which was associated with a high protein level and increase in the number of macrophages in the aqueous. We concluded that a phacolytic mechanism is responsible for a secondary rise of intraocular tension in cases of hypermature cataract.", "PMID": 965176}
{"id": "pubmed23n0032_6191", "title": "Candida sepsis successfully treated by parenteral administration of 5-fluorocytosine.", "content": "Candida sepsis has become one of the most common and dangerous forms of hospital acquired infection. The recommended drug for parenteral treatment of Candida sepsis is amphotericin B, however, its toxic effects preclude its usage in many patients, particularly in the presence of renal failure. A less toxic antifungal agent is 5-fluorocytosine. A patient with Candida albicans sepsis was treated successfully with 5-fluorocytosine by intravenous administration. The fungal infection developed during the course of acute renal failure, repeated surgical intervention, intravenous hyperalimentation, gastrointestinal bleeding and five months of antibiotic therapy. The clinical symptoms receded rapidly and cultures became sterile after one week of intravenous treatment. The predisposing factors, difficulties in prevention and diagnosis of fungal infection are discussed.", "contents": "Candida sepsis successfully treated by parenteral administration of 5-fluorocytosine. Candida sepsis has become one of the most common and dangerous forms of hospital acquired infection. The recommended drug for parenteral treatment of Candida sepsis is amphotericin B, however, its toxic effects preclude its usage in many patients, particularly in the presence of renal failure. A less toxic antifungal agent is 5-fluorocytosine. A patient with Candida albicans sepsis was treated successfully with 5-fluorocytosine by intravenous administration. The fungal infection developed during the course of acute renal failure, repeated surgical intervention, intravenous hyperalimentation, gastrointestinal bleeding and five months of antibiotic therapy. The clinical symptoms receded rapidly and cultures became sterile after one week of intravenous treatment. The predisposing factors, difficulties in prevention and diagnosis of fungal infection are discussed.", "PMID": 965177}
{"id": "pubmed23n0032_6192", "title": "Solid tumors of the liver. Diagnostic and therapeutic problems.", "content": "The diagnostic and therapeutic problems involved in 29 cases of isolated hepatic tumors have been presented. In nine cases the hepatoma was discovered during emergency treatment for complications. Selective arteriography was the most accurate method of establishing the etiologic diagnosis. Suprahepatic phlebography indicated the possibilities for surgical treatment. These possibilities are not influenced by the volume of the tumor, but are closely dependent on damage to the suprahepatic vascular system. Effective surgery was performed in 22 cases, of which 14 were excisions.", "contents": "Solid tumors of the liver. Diagnostic and therapeutic problems. The diagnostic and therapeutic problems involved in 29 cases of isolated hepatic tumors have been presented. In nine cases the hepatoma was discovered during emergency treatment for complications. Selective arteriography was the most accurate method of establishing the etiologic diagnosis. Suprahepatic phlebography indicated the possibilities for surgical treatment. These possibilities are not influenced by the volume of the tumor, but are closely dependent on damage to the suprahepatic vascular system. Effective surgery was performed in 22 cases, of which 14 were excisions.", "PMID": 965180}
{"id": "pubmed23n0032_6193", "title": "Malignant tumors of the thyroid.", "content": "Carcinoma of the thyroid gland is not common. The majority of lesions in this series were differentiated in type. The treatment of choice is almost total thyroidectomy supplemented by I131 in some cases. L-thyroxine was prescribed routinely for all patients postoperatively for replacement and depression treatment. The immediate postoperative mortality was about 1% and prognosis is relatively good. Undifferentiated thyroid carcinomas are fast growing and very lethal and unfortunately no method of treatment is effective. Only four patients in this group survived more than a year.", "contents": "Malignant tumors of the thyroid. Carcinoma of the thyroid gland is not common. The majority of lesions in this series were differentiated in type. The treatment of choice is almost total thyroidectomy supplemented by I131 in some cases. L-thyroxine was prescribed routinely for all patients postoperatively for replacement and depression treatment. The immediate postoperative mortality was about 1% and prognosis is relatively good. Undifferentiated thyroid carcinomas are fast growing and very lethal and unfortunately no method of treatment is effective. Only four patients in this group survived more than a year.", "PMID": 965182}
{"id": "pubmed23n0032_6194", "title": "Perineovulvovaginal preoperative preparation in minor gynecological surgery.", "content": "Fifty consecutive patients underwent minor elective gynecologic surgery. Most of them were from the low socioeconomic class. Twenty-five patients had their pubic, vulval and perineal hair shaved as part of the preoperative preparation. All patients underwent the same routine perineal, vulval and vaginal swabbing in the operating room. All patients were then examined for postoperative complications. Only two women (who were shaved) complained of mild lower abdominal pain 48 hours after operation, but neither had any clinical evidence of genital or urinary infection. Their symptoms disappeared with the use of analgesics. Even in developing countries where patients with poor personal hygiene are common, preoperative vulval, pubic and perineal hair shaving prior to minor gynecologic surgery is unnecessary. We suggest that this procedure should be discontinued.", "contents": "Perineovulvovaginal preoperative preparation in minor gynecological surgery. Fifty consecutive patients underwent minor elective gynecologic surgery. Most of them were from the low socioeconomic class. Twenty-five patients had their pubic, vulval and perineal hair shaved as part of the preoperative preparation. All patients underwent the same routine perineal, vulval and vaginal swabbing in the operating room. All patients were then examined for postoperative complications. Only two women (who were shaved) complained of mild lower abdominal pain 48 hours after operation, but neither had any clinical evidence of genital or urinary infection. Their symptoms disappeared with the use of analgesics. Even in developing countries where patients with poor personal hygiene are common, preoperative vulval, pubic and perineal hair shaving prior to minor gynecologic surgery is unnecessary. We suggest that this procedure should be discontinued.", "PMID": 965183}
{"id": "pubmed23n0032_6195", "title": "Incidence and evolution of moles and the relationship to malignant melanoma in eastern India.", "content": "Malignant melanoma is rather uncommon in India. Pigmented moles are also less common than among Caucasians. The fewer number of moles probably is one factor responsible for the relative rarity of malignant melanomas in this area.", "contents": "Incidence and evolution of moles and the relationship to malignant melanoma in eastern India. Malignant melanoma is rather uncommon in India. Pigmented moles are also less common than among Caucasians. The fewer number of moles probably is one factor responsible for the relative rarity of malignant melanomas in this area.", "PMID": 965185}
{"id": "pubmed23n0032_6196", "title": "Hormonal therapy and cholelithiasis.", "content": "This report is to alert the abdominal surgeon to a new factor responsible for a rising incidence of cholelithiasis in the younger woman. The cause of cholelithiasis has been explained on the basis of biochemical changes with mechanical obstruction. The role of estrogen-progesterone elevation in pregnancy with associated hypercholesteremia is well-known. Little known are reports (more common in non-American literature) that female hormones effect a sluggishness of gallbladder function. Thus, a chemical change with a functional type of obstruction in the biliary tree predisposes toward cholelithiasis. A survey of 1346 cholecystic operations from 1969 through 1973 showed the expected greater incidence in women. Women under 40 years of age who received hormonal medication were younger when operated upon than a comparable group not receiving hormonal therapy. The incidence of gallbladder disease in young women using hormonal therapy should be expected to increase in the coming years. Cholelithiasis should be suspected in the young female taking birth control medication. X-ray study is indicated and early surgery is desirable to prevent morbidity, mortality and the complications of cholecystic disease.", "contents": "Hormonal therapy and cholelithiasis. This report is to alert the abdominal surgeon to a new factor responsible for a rising incidence of cholelithiasis in the younger woman. The cause of cholelithiasis has been explained on the basis of biochemical changes with mechanical obstruction. The role of estrogen-progesterone elevation in pregnancy with associated hypercholesteremia is well-known. Little known are reports (more common in non-American literature) that female hormones effect a sluggishness of gallbladder function. Thus, a chemical change with a functional type of obstruction in the biliary tree predisposes toward cholelithiasis. A survey of 1346 cholecystic operations from 1969 through 1973 showed the expected greater incidence in women. Women under 40 years of age who received hormonal medication were younger when operated upon than a comparable group not receiving hormonal therapy. The incidence of gallbladder disease in young women using hormonal therapy should be expected to increase in the coming years. Cholelithiasis should be suspected in the young female taking birth control medication. X-ray study is indicated and early surgery is desirable to prevent morbidity, mortality and the complications of cholecystic disease.", "PMID": 965186}
{"id": "pubmed23n0032_6197", "title": "Pattern of acute head injuries in Ibadan.", "content": "A series of 525 patients with head injuries was analyzed. Males and young people, especially children under 10, predominated. More injuries were sustained on Sunday than any other day, and were mostly caused by road traffic accidents and domestic accidents. Limb injuries were the most common associated wounds. The more severely disturbed the level of consciousness on admission, the worse was the prognosis.", "contents": "Pattern of acute head injuries in Ibadan. A series of 525 patients with head injuries was analyzed. Males and young people, especially children under 10, predominated. More injuries were sustained on Sunday than any other day, and were mostly caused by road traffic accidents and domestic accidents. Limb injuries were the most common associated wounds. The more severely disturbed the level of consciousness on admission, the worse was the prognosis.", "PMID": 965188}
{"id": "pubmed23n0032_6198", "title": "Surgical management of pyogenic pericarditis.", "content": "Sixteen cases of acute pyogenic pericarditis have been reviewed. Uncontrollable septic course, episodes of cardiac tamponade and failure of the nonsurgical regimen were the main indications for surgery. Medical management is often ineffective because of the relentless septic course, presence of thick, viscid pus and granulation tissue, and loculation. Even if it is effective, it may not halt the progression to constriction. We treated these cases by emergency pericardiectomy. The results were gratifying, the risk of cardiac tamponade was eliminated and termination of the septic course was dramatic. The mortality rate was within acceptable limits. All the survivors have remained asymptomatic.", "contents": "Surgical management of pyogenic pericarditis. Sixteen cases of acute pyogenic pericarditis have been reviewed. Uncontrollable septic course, episodes of cardiac tamponade and failure of the nonsurgical regimen were the main indications for surgery. Medical management is often ineffective because of the relentless septic course, presence of thick, viscid pus and granulation tissue, and loculation. Even if it is effective, it may not halt the progression to constriction. We treated these cases by emergency pericardiectomy. The results were gratifying, the risk of cardiac tamponade was eliminated and termination of the septic course was dramatic. The mortality rate was within acceptable limits. All the survivors have remained asymptomatic.", "PMID": 965190}
{"id": "pubmed23n0032_6199", "title": "Triple isotope scintiphotography for differentiating between renal cysts and renal tumors.", "content": "As a routine diagnostic approach to patients who present hematuria or abnormal findings in X-ray films such as intravenous pyelograms, the cases are submitted to serial scintiphotography every two minutes after the intravenous administration of 250 muCi of 131I-Hippuran and to renography with PHO/GAMMA Scintillation Camera (Nuclear-Chicago). When scintiphotography with 131I-Hippuran reveals filling defect in the kidney region, scintiphotography with 150 muCi of 203Hg-chlormerodrin and renal blood flow scintiphotography after antecubital intravenous injection of 10 mCi of 99mTc are carried out. The present report concerns 8 cases of renal tumor and 5 cases of renal cyst confirmed by X-ray examination and surgical operation. The scintiphotography revealed decreased uptake of 131I-Hippuran and 203Hg-chlormerodrin in both tumor and cyst sites, exhibiting round or band-shaped filling defects. On the other hand, renal blood flow scintiphotography with 99mTc demonstrated the cyst as a filling defect but failed to visualize most of tumors. Accordingly, these techniques are available for differential diagnosis of the two lesions.", "contents": "Triple isotope scintiphotography for differentiating between renal cysts and renal tumors. As a routine diagnostic approach to patients who present hematuria or abnormal findings in X-ray films such as intravenous pyelograms, the cases are submitted to serial scintiphotography every two minutes after the intravenous administration of 250 muCi of 131I-Hippuran and to renography with PHO/GAMMA Scintillation Camera (Nuclear-Chicago). When scintiphotography with 131I-Hippuran reveals filling defect in the kidney region, scintiphotography with 150 muCi of 203Hg-chlormerodrin and renal blood flow scintiphotography after antecubital intravenous injection of 10 mCi of 99mTc are carried out. The present report concerns 8 cases of renal tumor and 5 cases of renal cyst confirmed by X-ray examination and surgical operation. The scintiphotography revealed decreased uptake of 131I-Hippuran and 203Hg-chlormerodrin in both tumor and cyst sites, exhibiting round or band-shaped filling defects. On the other hand, renal blood flow scintiphotography with 99mTc demonstrated the cyst as a filling defect but failed to visualize most of tumors. Accordingly, these techniques are available for differential diagnosis of the two lesions.", "PMID": 965194}
{"id": "pubmed23n0032_6200", "title": "Four-channel radioisotope renography (complex investigations).", "content": "An attempt has been made at a fuller utilization of the information content of 131I-Hippurate in four-channel renography, recording the activities over the kidneys, heart and bladder. The four-channel system was supplemented with a digital scaler, which enabled also the volume of residual urine to be determined after renography. In functional disorder of the upper outflow tract, the examinations were performed in both supine and sitting positions. The expanded procedure has rendered the familiar method of renography more valuable and has often given essential diagnostic help when used in combination with other clinical data.", "contents": "Four-channel radioisotope renography (complex investigations). An attempt has been made at a fuller utilization of the information content of 131I-Hippurate in four-channel renography, recording the activities over the kidneys, heart and bladder. The four-channel system was supplemented with a digital scaler, which enabled also the volume of residual urine to be determined after renography. In functional disorder of the upper outflow tract, the examinations were performed in both supine and sitting positions. The expanded procedure has rendered the familiar method of renography more valuable and has often given essential diagnostic help when used in combination with other clinical data.", "PMID": 965195}
{"id": "pubmed23n0032_6201", "title": "Renal colic and associated pyelorenal reflux as a cause of some diseases.", "content": "Renal colic always involves pelvic hypertension and severe urodynamic disturbances which result in fornical reflux, urine leakage to renal interstitial tissues, to tissues of the renal sinus, and sometimes to the retroperitoneal space. If the urinary tract is infected, renal colic may lead to acute pyelonephritis and generalized infection, as well as to bacteriemic shock. Of great importance in the origin of retroperitoneal fibrosis are fornical refluxes. Since renal colic is the most frequent cause of pyelorenal reflux, patients with renal colic should be promptly given appropriate treatment aimed at eliminating or at least lowering pelvic hypertension.", "contents": "Renal colic and associated pyelorenal reflux as a cause of some diseases. Renal colic always involves pelvic hypertension and severe urodynamic disturbances which result in fornical reflux, urine leakage to renal interstitial tissues, to tissues of the renal sinus, and sometimes to the retroperitoneal space. If the urinary tract is infected, renal colic may lead to acute pyelonephritis and generalized infection, as well as to bacteriemic shock. Of great importance in the origin of retroperitoneal fibrosis are fornical refluxes. Since renal colic is the most frequent cause of pyelorenal reflux, patients with renal colic should be promptly given appropriate treatment aimed at eliminating or at least lowering pelvic hypertension.", "PMID": 965197}
{"id": "pubmed23n0032_6202", "title": "The angiographic appearance of renal angiomyolipoma and problems of its preoperative diagnosis.", "content": "The radiodiagnostic difficulties of renal hamartoma (renal angiomyolipoma) have been discussed in connection with the authors' two cases. In the first case a unifocal cystic renal angiomyolipoma without tuberous sclerosis caused retroperitoneal haematoma and acute symptoms. In the second case a preoperative tentative diagnosis of angiomyolipoma was made, however, the Pringle-type dermal lesions were only observed 6 months after surgery on control examination. The authors state that the radiographic functional findings vary depending on the tissue structure of the tumour. Differential diagnosis of cystic angiomyolipoma containing little fatty and angiomatous tissue is impossible, whereas for the hypervascular variant a tentative diagnosis can be established even in the case of unifocal hamartoma.", "contents": "The angiographic appearance of renal angiomyolipoma and problems of its preoperative diagnosis. The radiodiagnostic difficulties of renal hamartoma (renal angiomyolipoma) have been discussed in connection with the authors' two cases. In the first case a unifocal cystic renal angiomyolipoma without tuberous sclerosis caused retroperitoneal haematoma and acute symptoms. In the second case a preoperative tentative diagnosis of angiomyolipoma was made, however, the Pringle-type dermal lesions were only observed 6 months after surgery on control examination. The authors state that the radiographic functional findings vary depending on the tissue structure of the tumour. Differential diagnosis of cystic angiomyolipoma containing little fatty and angiomatous tissue is impossible, whereas for the hypervascular variant a tentative diagnosis can be established even in the case of unifocal hamartoma.", "PMID": 965196}
{"id": "pubmed23n0032_6203", "title": "Results of operative treatment of vesicoureteral reflux by the Leadbetter-Politano method in children.", "content": "In this work the results of vesicoureteroplasty performed by the Leadbetter-Politano method in 77 children are presented. The indications for a vesicoureteroplasty procedure are outlined. Bad results are analysed and the causes of failure and possibilities of preventing them are considered. Probable causes of vesicoureteral reflux on the side opposite to the operated one, and the influence of coexisting congenital anomalies of urinary tract upon operative results are discussed.", "contents": "Results of operative treatment of vesicoureteral reflux by the Leadbetter-Politano method in children. In this work the results of vesicoureteroplasty performed by the Leadbetter-Politano method in 77 children are presented. The indications for a vesicoureteroplasty procedure are outlined. Bad results are analysed and the causes of failure and possibilities of preventing them are considered. Probable causes of vesicoureteral reflux on the side opposite to the operated one, and the influence of coexisting congenital anomalies of urinary tract upon operative results are discussed.", "PMID": 965198}
{"id": "pubmed23n0032_6204", "title": "Congenital stricture of male urethra.", "content": "Seven cases of anterior urethral stricture were studied. The diagnosis was made by exclusion of the inflammatory and traumatic causes. In three cases the stricture was located in the bulbar and in four cases in the penile urethra. The results obtained were good, with a follow-up of 5 to 50 months. Considerations are made about embryogenic, diagnostic, clinical picture and treatment. The verification of extensive lesions of the urethra determined the use of more complex surgical techniques of Johansen's urethroplasty devised for the treatment of this kind of pathology.", "contents": "Congenital stricture of male urethra. Seven cases of anterior urethral stricture were studied. The diagnosis was made by exclusion of the inflammatory and traumatic causes. In three cases the stricture was located in the bulbar and in four cases in the penile urethra. The results obtained were good, with a follow-up of 5 to 50 months. Considerations are made about embryogenic, diagnostic, clinical picture and treatment. The verification of extensive lesions of the urethra determined the use of more complex surgical techniques of Johansen's urethroplasty devised for the treatment of this kind of pathology.", "PMID": 965199}
{"id": "pubmed23n0032_6205", "title": "Piromidic acid and its ethylic ester: absorption, distribution, excretion.", "content": "The absorption, distribution and excretion of piromidic acid and its ethyl ester were investigated in the rat. When administered orally the ethyl ester was well absorbed and subsequently hydrolysed, giving much higher blood and tissue concentration than a corresponding dose of the parent compound. The antimicrobial activity of urine and bile samples was also investigated.", "contents": "Piromidic acid and its ethylic ester: absorption, distribution, excretion. The absorption, distribution and excretion of piromidic acid and its ethyl ester were investigated in the rat. When administered orally the ethyl ester was well absorbed and subsequently hydrolysed, giving much higher blood and tissue concentration than a corresponding dose of the parent compound. The antimicrobial activity of urine and bile samples was also investigated.", "PMID": 965200}
{"id": "pubmed23n0032_6206", "title": "Appendectomies in patients with nephritis.", "content": "A retrospective study of 150 records of patients attending a renal clinic revealed a high (10 per cent) incidence of history of appendectomy. The possibility that an acute exacerbation of pyelonephritis, especially on the right side, might be misdiagnosed in some cases as appendicitis is discussed. Acute renal failure following unnecessary appendectomy in a patient with previously unrecognized and asymptomatic chronic renal disease may occur if this possibility is not borne in mind before operating on an atypical case of appendicitis.", "contents": "Appendectomies in patients with nephritis. A retrospective study of 150 records of patients attending a renal clinic revealed a high (10 per cent) incidence of history of appendectomy. The possibility that an acute exacerbation of pyelonephritis, especially on the right side, might be misdiagnosed in some cases as appendicitis is discussed. Acute renal failure following unnecessary appendectomy in a patient with previously unrecognized and asymptomatic chronic renal disease may occur if this possibility is not borne in mind before operating on an atypical case of appendicitis.", "PMID": 965201}
{"id": "pubmed23n0032_6207", "title": "Use of absorbable material for the closure of experimental longitudinal nephrotomy.", "content": "Large nephrotomy incisions made in 15 dogs were closed by sewing up the cavity system with stitches of 5/0 catgut and by sealing the renal parenchyma with tissue adhesive being pinpointed at the opened vessels. The incision was covered with a preparation of oxidized cellulose which was then likewise impregnated with tissue adhesive. This technique was found rapid and convenient and provided for adequate haemostasis. Its essential advantage lies in eliminating the deep through-and-through sutures involving a major loss of functioning renal parenchyma. All substances used were absorbed in due time.", "contents": "Use of absorbable material for the closure of experimental longitudinal nephrotomy. Large nephrotomy incisions made in 15 dogs were closed by sewing up the cavity system with stitches of 5/0 catgut and by sealing the renal parenchyma with tissue adhesive being pinpointed at the opened vessels. The incision was covered with a preparation of oxidized cellulose which was then likewise impregnated with tissue adhesive. This technique was found rapid and convenient and provided for adequate haemostasis. Its essential advantage lies in eliminating the deep through-and-through sutures involving a major loss of functioning renal parenchyma. All substances used were absorbed in due time.", "PMID": 965203}
{"id": "pubmed23n0032_6208", "title": "Haemorrhagic pericarditis and cardiac tamponade during chronic haemodialysis.", "content": "Three cases of uraemic haemorrhagic pericarditis observed during haemodialysis treatment are described. The condition is explained with uraemic toxicosis, cyclic heparinaemia resulting from dialysis, deficient thrombocyte function, and decreased capillary resistance. The clinical appearance of haemorrhagic pericarditis was not typical in any of the cases. Two patients died, while in one patient partial pericardiectomy was performed with success.", "contents": "Haemorrhagic pericarditis and cardiac tamponade during chronic haemodialysis. Three cases of uraemic haemorrhagic pericarditis observed during haemodialysis treatment are described. The condition is explained with uraemic toxicosis, cyclic heparinaemia resulting from dialysis, deficient thrombocyte function, and decreased capillary resistance. The clinical appearance of haemorrhagic pericarditis was not typical in any of the cases. Two patients died, while in one patient partial pericardiectomy was performed with success.", "PMID": 965202}
{"id": "pubmed23n0032_6209", "title": "Method of choice for urinary diversion in surgical treatment of bladder carcinoma.", "content": "Evidence is presented concerning 94 patients with malignancy of bladder in whom diversion of urine was performed after cystectomy for exclusion of the bladder. There were 80 men and 14 women, ages ranging from 30 to 81 years. In 35 of these 94 cases ureterosigmoidoanastomosis, in 38 cases ureterocutaneostomy, in 15 Portilla's operation, in 2 Bricker's method and in 4 nephro-pyelostomy were performed. For the prevention of bleeding cystectomy was preceded by bilateral ligation of the internal iliac artery in 14 cases. Continuous postoperative follow-up of the patients (from a few months to 14 years) and analysis of the early and late results allow to regard the combined technique of Coffey II-Nesbit-Goodwin as the method of choice having the slightest risk of peritonitis, intestinoureteral reflux and other complications inherent in other procedures. Of the existing methods of ureterocutaneostomy the authors have chosen Le Dentu's method with some modifications. They consider ureterostomy to be the simplest and quickest method owing to the higher location of ureterostoma. Portilla's method is no longer used because of its unfavourable long-term results. Of 94 patients with cancer of the bladder, who had been subjected to different methods of urinary diversion in the years 1954-1974, 16 have survived up till now. There are 10 survivors after ureterosigmoidoanastomosis, 5 after ureterocutaneostomy and 1 after Bricker's operation.", "contents": "Method of choice for urinary diversion in surgical treatment of bladder carcinoma. Evidence is presented concerning 94 patients with malignancy of bladder in whom diversion of urine was performed after cystectomy for exclusion of the bladder. There were 80 men and 14 women, ages ranging from 30 to 81 years. In 35 of these 94 cases ureterosigmoidoanastomosis, in 38 cases ureterocutaneostomy, in 15 Portilla's operation, in 2 Bricker's method and in 4 nephro-pyelostomy were performed. For the prevention of bleeding cystectomy was preceded by bilateral ligation of the internal iliac artery in 14 cases. Continuous postoperative follow-up of the patients (from a few months to 14 years) and analysis of the early and late results allow to regard the combined technique of Coffey II-Nesbit-Goodwin as the method of choice having the slightest risk of peritonitis, intestinoureteral reflux and other complications inherent in other procedures. Of the existing methods of ureterocutaneostomy the authors have chosen Le Dentu's method with some modifications. They consider ureterostomy to be the simplest and quickest method owing to the higher location of ureterostoma. Portilla's method is no longer used because of its unfavourable long-term results. Of 94 patients with cancer of the bladder, who had been subjected to different methods of urinary diversion in the years 1954-1974, 16 have survived up till now. There are 10 survivors after ureterosigmoidoanastomosis, 5 after ureterocutaneostomy and 1 after Bricker's operation.", "PMID": 965204}
{"id": "pubmed23n0032_6210", "title": "Vascular changes in the urinary bladder wall after irradiation (an experimental study in rabbits with angiography and scanning electron microscopy of vascular casts).", "content": "Seven adult rabbits were used to study radiation-induced vascular changes in the urinary bladder by angiography and scanning electron microscopy of vascular casts. The angiographic studies, in which a magnification techniques was used, revealed a state of hypervascularization in the irradiated area of the bladder. With the angiographic method, however, it was not possible to visualize specific radiation-induced vascular changes within the irradiated area of the urinary bladder. Scanning electron microscopy of vascular casts made possible a detailed study of finer vessels in which typical radiation changes, teleangiectases, could be demonstrated.", "contents": "Vascular changes in the urinary bladder wall after irradiation (an experimental study in rabbits with angiography and scanning electron microscopy of vascular casts). Seven adult rabbits were used to study radiation-induced vascular changes in the urinary bladder by angiography and scanning electron microscopy of vascular casts. The angiographic studies, in which a magnification techniques was used, revealed a state of hypervascularization in the irradiated area of the bladder. With the angiographic method, however, it was not possible to visualize specific radiation-induced vascular changes within the irradiated area of the urinary bladder. Scanning electron microscopy of vascular casts made possible a detailed study of finer vessels in which typical radiation changes, teleangiectases, could be demonstrated.", "PMID": 965205}
{"id": "pubmed23n0032_6211", "title": "Hydrostatic pressure technique and intravesical instillation of formalin: new methods for the control of severe bleeding from the bladder.", "content": "Intractable haemorrhages of the bladder in consequence of wide-spread tumours or of haemorrhagic cystitis secondary to radiation therapy involve serious therapeutic problems. Failure of the conventional therapy to bring the haemorrhage under control imposes more active measures so as to avert the danger of exsanguination. The condition of the patients is, however, generally incompatible with major surgery. In this situation the authors resorted to the hydrostatic pressure technique and to intravesical instillations of formalin. Their observations with these procedures are reported.", "contents": "Hydrostatic pressure technique and intravesical instillation of formalin: new methods for the control of severe bleeding from the bladder. Intractable haemorrhages of the bladder in consequence of wide-spread tumours or of haemorrhagic cystitis secondary to radiation therapy involve serious therapeutic problems. Failure of the conventional therapy to bring the haemorrhage under control imposes more active measures so as to avert the danger of exsanguination. The condition of the patients is, however, generally incompatible with major surgery. In this situation the authors resorted to the hydrostatic pressure technique and to intravesical instillations of formalin. Their observations with these procedures are reported.", "PMID": 965206}
{"id": "pubmed23n0032_6212", "title": "Liver function in porstatic carcinoma patients.", "content": "Liver function tests were carried out for serum zinc turbidity test, icterus index, alkaline phosphatase, bilirubin and transaminases in a series of 222 patients, of whom 119 had prostatic carcinoma, 63 benign hyperplasia of the prostate and 40 congenital abnormality without prostatic trouble. Patients having prostatic carcinoma often displayed abnormally high levels of zinc turbidity test, alkaline phosphatase, bilirubin and transaminases.", "contents": "Liver function in porstatic carcinoma patients. Liver function tests were carried out for serum zinc turbidity test, icterus index, alkaline phosphatase, bilirubin and transaminases in a series of 222 patients, of whom 119 had prostatic carcinoma, 63 benign hyperplasia of the prostate and 40 congenital abnormality without prostatic trouble. Patients having prostatic carcinoma often displayed abnormally high levels of zinc turbidity test, alkaline phosphatase, bilirubin and transaminases.", "PMID": 965207}
{"id": "pubmed23n0032_6213", "title": "Single-needle haemodialysis.", "content": "The single-needle method, which greatly simplifies the technique of haemodialysis, was tested for its efficiency by in vitro and in vivo studies. It has proved equal in efficiency to the conventional double-needle techniques. The possibilities of unilateral puncture of the femoral vein in association with the single-needle technique are pointed out.", "contents": "Single-needle haemodialysis. The single-needle method, which greatly simplifies the technique of haemodialysis, was tested for its efficiency by in vitro and in vivo studies. It has proved equal in efficiency to the conventional double-needle techniques. The possibilities of unilateral puncture of the femoral vein in association with the single-needle technique are pointed out.", "PMID": 965208}
{"id": "pubmed23n0032_6214", "title": "Diagnostic value of ceruloplasmin, haptoglobin and sialic acid in chronic pyelonephritis.", "content": "In a group of 84 patients with exacerbated chronic pyelonephritis the authors have determined the blood serum levels of ceruloplasmin, haptoglobin and sialic acid before, during and after antibacterial treatment. They have found a decrease in these levels following treatment. The results of ceruloplasmin, haptoglobin and sialic acid assays could be used as an index for the activity of the pathologic process, as well as in the differential diagnosis between pyelonephritis and glomerulonephritis.", "contents": "Diagnostic value of ceruloplasmin, haptoglobin and sialic acid in chronic pyelonephritis. In a group of 84 patients with exacerbated chronic pyelonephritis the authors have determined the blood serum levels of ceruloplasmin, haptoglobin and sialic acid before, during and after antibacterial treatment. They have found a decrease in these levels following treatment. The results of ceruloplasmin, haptoglobin and sialic acid assays could be used as an index for the activity of the pathologic process, as well as in the differential diagnosis between pyelonephritis and glomerulonephritis.", "PMID": 965209}
{"id": "pubmed23n0032_6215", "title": "Peritoneal clearances in scleroderma and diabetes mellitus: effects of intraperitioneal isoproterenol.", "content": "Reduced peritoneal clearances of creatinine and urate were demonstrated repeatedly in a patient with scleroderma and a patient with diabetes mellitus. Urea clearances were not significantly different from usual values. The findings suggest decreased peritoneal membrane permeability and/or area (if urea clearance is flow limited). Clearances increased to usual values with intraperitioneal isoproterenol in the patient with diabetes. There was no effect of isoproterenol in the patient with scleroderma.", "contents": "Peritoneal clearances in scleroderma and diabetes mellitus: effects of intraperitioneal isoproterenol. Reduced peritoneal clearances of creatinine and urate were demonstrated repeatedly in a patient with scleroderma and a patient with diabetes mellitus. Urea clearances were not significantly different from usual values. The findings suggest decreased peritoneal membrane permeability and/or area (if urea clearance is flow limited). Clearances increased to usual values with intraperitioneal isoproterenol in the patient with diabetes. There was no effect of isoproterenol in the patient with scleroderma.", "PMID": 965210}
{"id": "pubmed23n0032_6216", "title": "Non-deflating Foley catheter.", "content": "The different methods of removal of a non-deflating ureteral catheter were compared with the stylet technique. Two hundred and sixty randomly selected catheters were tested. Overdistention of the balloon with water or air and chemically induced rupture of the balloon produced fragments of various sizes in almost 100%. Our stylet technique failed to demonstrate any of the complications reported in the literature i.e., chemical or mechanical injury to the bladder or retained rubber fragments causing stone formation.", "contents": "Non-deflating Foley catheter. The different methods of removal of a non-deflating ureteral catheter were compared with the stylet technique. Two hundred and sixty randomly selected catheters were tested. Overdistention of the balloon with water or air and chemically induced rupture of the balloon produced fragments of various sizes in almost 100%. Our stylet technique failed to demonstrate any of the complications reported in the literature i.e., chemical or mechanical injury to the bladder or retained rubber fragments causing stone formation.", "PMID": 965211}
{"id": "pubmed23n0032_6217", "title": "Malignant tumours of the renal pelvis.", "content": "A review of the clinical data of 22 patients with malignant tumours of the renal pelvis, accounting for 10% of the total patient material of renal tumours, is presented. The earliest manifestation was prevalently haematuria, less often pain. In two thirds of the cases the diagnosis was established on radiological evidence and in one third the process remained undetected until surgical exposure of the kidney. The diagnostic difficulties involved by tumours of the renal pelvis and the value of the diagnostic methods are discussed. Nephroureterectomy with excision of a cuff of bladder was carried out in 15, nephrectomy in 5 cases. Three of the 22 patients have survived five years. Early diagnosis followed by total nephroureterectomy without delay may bring some improvement in the poor prognosis of these tumours.", "contents": "Malignant tumours of the renal pelvis. A review of the clinical data of 22 patients with malignant tumours of the renal pelvis, accounting for 10% of the total patient material of renal tumours, is presented. The earliest manifestation was prevalently haematuria, less often pain. In two thirds of the cases the diagnosis was established on radiological evidence and in one third the process remained undetected until surgical exposure of the kidney. The diagnostic difficulties involved by tumours of the renal pelvis and the value of the diagnostic methods are discussed. Nephroureterectomy with excision of a cuff of bladder was carried out in 15, nephrectomy in 5 cases. Three of the 22 patients have survived five years. Early diagnosis followed by total nephroureterectomy without delay may bring some improvement in the poor prognosis of these tumours.", "PMID": 965212}
{"id": "pubmed23n0032_6218", "title": "Contribution of a nurse clinician to office practice productivity: comparison of two solo primary care practices.", "content": "A comparison of two solo primary care practices with similar patient populations reveals a significant difference in productivity. A nurse clinician was employed in the more productive practice. She independently managed 1,848 patient visits a year that would otherwise have required the time and attention of a physician. She contributed to the productivity of the physician by performing some tasks he would normally have performed during visits they managed jointly. It was primarily because of the assistance he received from the nurse clinician that the physician in practice II was 12 percent more productive than the physician in practice I. The nurse clinician and physician managed 31 percent more patient visits during a standard day than the physician in practice I, or a difference of 2,856 patient visits a year. This annual difference is based on a work schedule that could be matched in other practices: an eight-hour day and a 240-day work year.", "contents": "Contribution of a nurse clinician to office practice productivity: comparison of two solo primary care practices. A comparison of two solo primary care practices with similar patient populations reveals a significant difference in productivity. A nurse clinician was employed in the more productive practice. She independently managed 1,848 patient visits a year that would otherwise have required the time and attention of a physician. She contributed to the productivity of the physician by performing some tasks he would normally have performed during visits they managed jointly. It was primarily because of the assistance he received from the nurse clinician that the physician in practice II was 12 percent more productive than the physician in practice I. The nurse clinician and physician managed 31 percent more patient visits during a standard day than the physician in practice I, or a difference of 2,856 patient visits a year. This annual difference is based on a work schedule that could be matched in other practices: an eight-hour day and a 240-day work year.", "PMID": 965232}
{"id": "pubmed23n0032_6219", "title": "A managerial accounting analysis of hospital costs.", "content": "Variance analysis, an accounting technique, is applied to an eight-component model of hospital costs to determine the contribution each component makes to cost increases. The method is illustrated by application to data on total costs from 1950 to 1973 for all U.S. nongovernmental not-for-profit short-term general hospitals. The costs of a single hospital are analyzed and compared to the group costs. The potential uses and limitations of the method as a planning and research tool are discussed.", "contents": "A managerial accounting analysis of hospital costs. Variance analysis, an accounting technique, is applied to an eight-component model of hospital costs to determine the contribution each component makes to cost increases. The method is illustrated by application to data on total costs from 1950 to 1973 for all U.S. nongovernmental not-for-profit short-term general hospitals. The costs of a single hospital are analyzed and compared to the group costs. The potential uses and limitations of the method as a planning and research tool are discussed.", "PMID": 965233}
{"id": "pubmed23n0032_6220", "title": "Utility of the injury severity score: a confirmation.", "content": "Independent results obtained from Illinois Trauma Registry data confirm an earlier finding of a fixed, monotonic relationship between expected mortality and the Injury Severity Score (ISS) value of injuries sustained in both vehicular and nonvehicular incidents. Further results show the ISS measure to be a good indicator of hospitalization demand from accident victims and suggest that this variable can be used to control for variations in injury severity in comparative evaluations of EMS systems.", "contents": "Utility of the injury severity score: a confirmation. Independent results obtained from Illinois Trauma Registry data confirm an earlier finding of a fixed, monotonic relationship between expected mortality and the Injury Severity Score (ISS) value of injuries sustained in both vehicular and nonvehicular incidents. Further results show the ISS measure to be a good indicator of hospitalization demand from accident victims and suggest that this variable can be used to control for variations in injury severity in comparative evaluations of EMS systems.", "PMID": 965234}
{"id": "pubmed23n0032_6221", "title": "A staff allocation model for mental health facilities.", "content": "This article describes a model for allocating staff within a large psychiatric hospital. The model provides an objective framework within which one can test alternative staff operating policies before making critical decisions concerning the employment of one category of personnel as opposed to another. It is based on objective data describing patient needs and staff functioning patterns, rather than subjective opinions concerning staff deployment. Besides being useful for the short-term deployment of staff and budgetary resources, it can also be used as a long-range planning tool for testing modifications in policy decisions and budget proposals. The algorithm employed, mixed-integer linear programming, is readily available; computer costs and running time are relatively minimal.", "contents": "A staff allocation model for mental health facilities. This article describes a model for allocating staff within a large psychiatric hospital. The model provides an objective framework within which one can test alternative staff operating policies before making critical decisions concerning the employment of one category of personnel as opposed to another. It is based on objective data describing patient needs and staff functioning patterns, rather than subjective opinions concerning staff deployment. Besides being useful for the short-term deployment of staff and budgetary resources, it can also be used as a long-range planning tool for testing modifications in policy decisions and budget proposals. The algorithm employed, mixed-integer linear programming, is readily available; computer costs and running time are relatively minimal.", "PMID": 965235}
{"id": "pubmed23n0032_6222", "title": "Determinants of output in group and solo medical practice.", "content": "Certain structural and environmental factors other than technical combination of resources and firm size are hypothesized to affect medical practice output. Four groups of variables related to physician attributes and activities, practice organization and patient characteristics, community characteristics, and factors specific to medical groups are examined by regression for correlation with two measures of practice output: gross revenue and total patient visits. Some tentative conclusions are discussed in relation to policies that might increase practice output.", "contents": "Determinants of output in group and solo medical practice. Certain structural and environmental factors other than technical combination of resources and firm size are hypothesized to affect medical practice output. Four groups of variables related to physician attributes and activities, practice organization and patient characteristics, community characteristics, and factors specific to medical groups are examined by regression for correlation with two measures of practice output: gross revenue and total patient visits. Some tentative conclusions are discussed in relation to policies that might increase practice output.", "PMID": 965236}
{"id": "pubmed23n0032_6223", "title": "[The management of sudden deafness (author's transl)].", "content": "The mean hearing gain (125-8,000 Hz) in 46 cases of sudden deafness treated with low molecular weight dextran was found to be significantly greater (20.9 dB) than that obtained in twelve cases treated differently (9.4 dB). These alternate measures of care included stellate ganglion blockade, vasoactive drugs, sodium bicarbonate infusions, and CO2-insufflation. Early diagnosis and treatment are considered to be crucial for successful therapy: the mean hearing gain in those patients who came for dextran treatment later than one week was less than half of the gain that resulted after treatment beginning within the first week. The results obtained conform with the hypothesis of cochlear microcirculation impairment or \"sludging\" as the prevalent cause of sudden deafness.", "contents": "[The management of sudden deafness (author's transl)]. The mean hearing gain (125-8,000 Hz) in 46 cases of sudden deafness treated with low molecular weight dextran was found to be significantly greater (20.9 dB) than that obtained in twelve cases treated differently (9.4 dB). These alternate measures of care included stellate ganglion blockade, vasoactive drugs, sodium bicarbonate infusions, and CO2-insufflation. Early diagnosis and treatment are considered to be crucial for successful therapy: the mean hearing gain in those patients who came for dextran treatment later than one week was less than half of the gain that resulted after treatment beginning within the first week. The results obtained conform with the hypothesis of cochlear microcirculation impairment or \"sludging\" as the prevalent cause of sudden deafness.", "PMID": 965258}
{"id": "pubmed23n0032_6224", "title": "[The facial nerve and the parotid gland (author's transl)].", "content": "The modern histological classification of epithelial salivary gland tumours is described. An exact knowledge of the behaviour of each type of tumour permits correct surgical planning. Contrary to the teaching that the superficial parotidectomy is the minimal safe resection, the author feels that in many cases dependent on tumour site, size and histological type, a simple excision is sufficient. The application of microsurgical techniques permits careful and safe surgery.", "contents": "[The facial nerve and the parotid gland (author's transl)]. The modern histological classification of epithelial salivary gland tumours is described. An exact knowledge of the behaviour of each type of tumour permits correct surgical planning. Contrary to the teaching that the superficial parotidectomy is the minimal safe resection, the author feels that in many cases dependent on tumour site, size and histological type, a simple excision is sufficient. The application of microsurgical techniques permits careful and safe surgery.", "PMID": 965259}
{"id": "pubmed23n0032_6225", "title": "[Bacterial airborne dispersal during the drilling of infected bone (author's transl)].", "content": "The hazards of infection by bacterial airborne dispersal secondary to drilling bone in the surgical treatment of infection of the air spaces of the skull were assessed experimentally. This included estimation of the furthest distance that the cooling fluid, using coloured water, and the bone chips of a dry petrous temporal bone can be thrown, and the spread of the fine dust produced by the drilling using a staph. albus suspension as the rinsing fluid. The drops and bone chips were flung to a distance of about 90 cm in the working area around a cutting burr rotating at 80 000 r.p.m. The fine dust thrown off by cutting and diamond burrs and the infected fluid mist stays suspended in the air for hours and can be carried across the whole room by air currents. The surgeon and operating theatre personnel are therefore always at risk of infection. Preventive measures are discussed.", "contents": "[Bacterial airborne dispersal during the drilling of infected bone (author's transl)]. The hazards of infection by bacterial airborne dispersal secondary to drilling bone in the surgical treatment of infection of the air spaces of the skull were assessed experimentally. This included estimation of the furthest distance that the cooling fluid, using coloured water, and the bone chips of a dry petrous temporal bone can be thrown, and the spread of the fine dust produced by the drilling using a staph. albus suspension as the rinsing fluid. The drops and bone chips were flung to a distance of about 90 cm in the working area around a cutting burr rotating at 80 000 r.p.m. The fine dust thrown off by cutting and diamond burrs and the infected fluid mist stays suspended in the air for hours and can be carried across the whole room by air currents. The surgeon and operating theatre personnel are therefore always at risk of infection. Preventive measures are discussed.", "PMID": 965260}
{"id": "pubmed23n0032_6226", "title": "[Deafness due to topical neomycin (author's transl)].", "content": "Today neomycin is almost exclusively given as an ointment in combination with other antibiotics, because of its known oto- and nephrotoxicity. Topical application in high dosage can cause ototoxicity as proved by one patient from this clinic. After laryngectomy and neck dissection for an extensive carcinoma of the epiglottis a 2 X 2 cm dehiscent pyocyaneus infected wound was treated daily with neomycin sulphate solution. A total of 30 gm was given over 3 months during which time the patient became deaf neomycin caused the deafness.", "contents": "[Deafness due to topical neomycin (author's transl)]. Today neomycin is almost exclusively given as an ointment in combination with other antibiotics, because of its known oto- and nephrotoxicity. Topical application in high dosage can cause ototoxicity as proved by one patient from this clinic. After laryngectomy and neck dissection for an extensive carcinoma of the epiglottis a 2 X 2 cm dehiscent pyocyaneus infected wound was treated daily with neomycin sulphate solution. A total of 30 gm was given over 3 months during which time the patient became deaf neomycin caused the deafness.", "PMID": 965261}
{"id": "pubmed23n0032_6227", "title": "[The significance of tympanometry in pediatric audiometry (author's transl)].", "content": "Audiological findings through tympanometry and audiometry were correlated with pathological findings involving the tympanic membranes and the nasopharynx in 232 patients (406 ears) with various hearing disorders in order to determine the probability of a conductive hearing deficit when only tympanogram and clinical findings are known. These measures hold special import to patients in whom exact evaluations of pure tone hearing thresholds are not possible (ie, in small infants and in patients with severe hearing losses in low frequency ranges).", "contents": "[The significance of tympanometry in pediatric audiometry (author's transl)]. Audiological findings through tympanometry and audiometry were correlated with pathological findings involving the tympanic membranes and the nasopharynx in 232 patients (406 ears) with various hearing disorders in order to determine the probability of a conductive hearing deficit when only tympanogram and clinical findings are known. These measures hold special import to patients in whom exact evaluations of pure tone hearing thresholds are not possible (ie, in small infants and in patients with severe hearing losses in low frequency ranges).", "PMID": 965262}
{"id": "pubmed23n0032_6228", "title": "[Results of otoplasty (author's transl)].", "content": "77 patients with uni- or bilateral otoplasty have been reviewed between 6 months and 5 years postoperatively. Surgery had been performed using a modified Converse technique. A comparison was made between the objective results and the patient's satisfaction. Using our own classification, we listed 64.9% as good results and 3.9% as failures, suggesting a reoperation. 31.2% of the cases demonstrated only some improvement. Evaluating our results listed as \"good\", the average distance helix: planum mastoideum, measured between the upper and middle third of the ear, was 18.8 mm and between the middle and lower third 18.0 mm.", "contents": "[Results of otoplasty (author's transl)]. 77 patients with uni- or bilateral otoplasty have been reviewed between 6 months and 5 years postoperatively. Surgery had been performed using a modified Converse technique. A comparison was made between the objective results and the patient's satisfaction. Using our own classification, we listed 64.9% as good results and 3.9% as failures, suggesting a reoperation. 31.2% of the cases demonstrated only some improvement. Evaluating our results listed as \"good\", the average distance helix: planum mastoideum, measured between the upper and middle third of the ear, was 18.8 mm and between the middle and lower third 18.0 mm.", "PMID": 965263}
{"id": "pubmed23n0032_6229", "title": "[Tracheal stenosis in children and its operative management (author's transl)].", "content": "The problem of tracheal stenosis in children is discussed, with emphasis placed on subglottic disease. The primary etiologies for the stenosis were chronic intubation and emergency tracheostomies following accidents. The operative technique described utilizes vertical division of the trachea and cricoid in the midline. The margins are then stretched laterally and sutured to skin incision lines. Granulations, scar and dislocated cartilage are removed, and the denuded area covered with free mucosal grafts or with pedicle skin flaps. After reestablishing an adequate airway (with use of an acrylic prosthesis for several weeks), the tracheal groove is closed with a composite graft from the auricle. A tracheostomy is left in place an additional 2-3 weeks, while the raw neck area is resurfaced by a transposition flap from adjacent skin. The technique has worked well since 1971 in three children (aged 3, 5 and 7 years), and was also performed successfully on five adult patients.", "contents": "[Tracheal stenosis in children and its operative management (author's transl)]. The problem of tracheal stenosis in children is discussed, with emphasis placed on subglottic disease. The primary etiologies for the stenosis were chronic intubation and emergency tracheostomies following accidents. The operative technique described utilizes vertical division of the trachea and cricoid in the midline. The margins are then stretched laterally and sutured to skin incision lines. Granulations, scar and dislocated cartilage are removed, and the denuded area covered with free mucosal grafts or with pedicle skin flaps. After reestablishing an adequate airway (with use of an acrylic prosthesis for several weeks), the tracheal groove is closed with a composite graft from the auricle. A tracheostomy is left in place an additional 2-3 weeks, while the raw neck area is resurfaced by a transposition flap from adjacent skin. The technique has worked well since 1971 in three children (aged 3, 5 and 7 years), and was also performed successfully on five adult patients.", "PMID": 965264}
{"id": "pubmed23n0032_6230", "title": "[The use of microlaryngoscopy in phoniatry (author's transl)].", "content": "Definite diagnostic advantages exist in the use of combined microlaryngoscopy and microstroboscopy in vocal rehbilitation since objective determination of vocal pathology can be made. The phoniatric considerations used in determining the indications for operative investigation are described. Reference is made to specific clinical problems, including the \"hormone voice\" in the Reinke edema from medications used in therapy and arytenoid dislocations from insufflation anesthesias. The postoperative prognosis after endolaryngeal surgery depends on the effectiveness of the surgical intervetion and the appropriate phoniatric-logopedic care employed. The author suggests that post-operative voice pauses should be minimized and not depend upon complete re-epithelialization of operative sites. Phoniatric and logopedic treatment used is based on considerations for appropriate vocal cord movements. The advantages of endolaryngeal faradization for vocal rehabilitation are discussed. In addition, the theoretical considerations of fitness for work are also discussed.", "contents": "[The use of microlaryngoscopy in phoniatry (author's transl)]. Definite diagnostic advantages exist in the use of combined microlaryngoscopy and microstroboscopy in vocal rehbilitation since objective determination of vocal pathology can be made. The phoniatric considerations used in determining the indications for operative investigation are described. Reference is made to specific clinical problems, including the \"hormone voice\" in the Reinke edema from medications used in therapy and arytenoid dislocations from insufflation anesthesias. The postoperative prognosis after endolaryngeal surgery depends on the effectiveness of the surgical intervetion and the appropriate phoniatric-logopedic care employed. The author suggests that post-operative voice pauses should be minimized and not depend upon complete re-epithelialization of operative sites. Phoniatric and logopedic treatment used is based on considerations for appropriate vocal cord movements. The advantages of endolaryngeal faradization for vocal rehabilitation are discussed. In addition, the theoretical considerations of fitness for work are also discussed.", "PMID": 965266}
{"id": "pubmed23n0032_6231", "title": "[Operative repair of the traumatically disrupted ossicular chain (author's transl)].", "content": "Consequent to the large number of skull injuries following traffic accidents, a significant number of patients experience traumatic disruption of the ossicular chain. Although most of these injuries can be managed by standard otologic procedures, this communication deals with three special clinical problems. Although not previously encountered, the diagnosis in the first could be made by otoscopy. In the second, infracture of the malleolar head had produced injury to the facial nerve which was exposed in its horizontal course. Although transposition of the malleus was required in repair, significant interference with hearing had not occurred since a spontaneous tympanoincudopexy had been produced by the injury.", "contents": "[Operative repair of the traumatically disrupted ossicular chain (author's transl)]. Consequent to the large number of skull injuries following traffic accidents, a significant number of patients experience traumatic disruption of the ossicular chain. Although most of these injuries can be managed by standard otologic procedures, this communication deals with three special clinical problems. Although not previously encountered, the diagnosis in the first could be made by otoscopy. In the second, infracture of the malleolar head had produced injury to the facial nerve which was exposed in its horizontal course. Although transposition of the malleus was required in repair, significant interference with hearing had not occurred since a spontaneous tympanoincudopexy had been produced by the injury.", "PMID": 965267}
{"id": "pubmed23n0032_6232", "title": "[Dysphonia in acromegaly (author's transl)].", "content": "Acromegalic dysphonia is described in a series of 80 acromegalic patients. Subjective dysphonia was observed in most patients, objective dysphonia, however, wasnoted in only 50% of the cases. The patients with objective dysphonia usually had progenia, moderate to high acromegalic activity and duration of disease of more than five years. The different factors of importance for the development of acromegalic dysphonia are summarized.", "contents": "[Dysphonia in acromegaly (author's transl)]. Acromegalic dysphonia is described in a series of 80 acromegalic patients. Subjective dysphonia was observed in most patients, objective dysphonia, however, wasnoted in only 50% of the cases. The patients with objective dysphonia usually had progenia, moderate to high acromegalic activity and duration of disease of more than five years. The different factors of importance for the development of acromegalic dysphonia are summarized.", "PMID": 965269}
{"id": "pubmed23n0032_6233", "title": "[Management of median cysts and fistulae (author's transl)].", "content": "The total extirpation of thyroglossal duct remnants with resection of the mid hyoid bone is reported as the only method for successful surgical therapy. Convoluted ducts of cysts and fistulae must be dissected to the base of the tongue and excised. As our cases demonstrate, recurrences will be avoided and revision surgery, when required, will be most successful.", "contents": "[Management of median cysts and fistulae (author's transl)]. The total extirpation of thyroglossal duct remnants with resection of the mid hyoid bone is reported as the only method for successful surgical therapy. Convoluted ducts of cysts and fistulae must be dissected to the base of the tongue and excised. As our cases demonstrate, recurrences will be avoided and revision surgery, when required, will be most successful.", "PMID": 965270}
{"id": "pubmed23n0032_6234", "title": "[Puncture of the maxillary sinus (author's transl)].", "content": "In some texts on operative technique it is recommended, when puncturing the maxillary sinus via the inferior meatus, to aim towards the outer canthus of the eye. It is demonstrated on a skull that aiming at the inner canthus avoids the danger of piercing the premaxillary tissue and in addition the diameter of the sinus is greater at this angle.", "contents": "[Puncture of the maxillary sinus (author's transl)]. In some texts on operative technique it is recommended, when puncturing the maxillary sinus via the inferior meatus, to aim towards the outer canthus of the eye. It is demonstrated on a skull that aiming at the inner canthus avoids the danger of piercing the premaxillary tissue and in addition the diameter of the sinus is greater at this angle.", "PMID": 965271}
{"id": "pubmed23n0032_6235", "title": "[Treatment of Meni\u00e8re's disease with betahistine (author's transl)].", "content": "Betahistine was gien to 86 patients with Meniere's disease in a thric daily dose of 8 mg. All patients except for three had earlier been treated medically without success. Symptomatic improvement occurred overall in 82,5%, including in 66% ear pressure symptoms, in 59.5% headache in 57% tinnitus, and in 35% hearing, although this latter statistically was not confirmed. The duration of administration of betahistine is therapeutically important and benefit from 4 months' continued use was demonstrated. After stopping treatment, the therapeutic effects were maintained and more than 6 months later improvement in hearing and ear pressure symptoms was still recorded. There was though a deterioration in symptoms of vertigo, tinnitus and headache in 18% over the same period. The shorter the duration of the symptoms the better the therapeutic effect gained. Improvement estimations of 81% in patients affected for up to 1 month, of 65% in patients affected from 1 to 12 months, and of 50% in patients affected more than one year were obtained. These results compare averagedly with the widely varible results of others. The management of Meniere's disease with betahistine can be regarded as an improvement in the conservative therapy of this disorder.", "contents": "[Treatment of Meni\u00e8re's disease with betahistine (author's transl)]. Betahistine was gien to 86 patients with Meniere's disease in a thric daily dose of 8 mg. All patients except for three had earlier been treated medically without success. Symptomatic improvement occurred overall in 82,5%, including in 66% ear pressure symptoms, in 59.5% headache in 57% tinnitus, and in 35% hearing, although this latter statistically was not confirmed. The duration of administration of betahistine is therapeutically important and benefit from 4 months' continued use was demonstrated. After stopping treatment, the therapeutic effects were maintained and more than 6 months later improvement in hearing and ear pressure symptoms was still recorded. There was though a deterioration in symptoms of vertigo, tinnitus and headache in 18% over the same period. The shorter the duration of the symptoms the better the therapeutic effect gained. Improvement estimations of 81% in patients affected for up to 1 month, of 65% in patients affected from 1 to 12 months, and of 50% in patients affected more than one year were obtained. These results compare averagedly with the widely varible results of others. The management of Meniere's disease with betahistine can be regarded as an improvement in the conservative therapy of this disorder.", "PMID": 965272}
{"id": "pubmed23n0032_6236", "title": "The fistula and Hennebert tests.", "content": "The fistula and Hennebert tests are used to detect the structural integrity of the bony labyrinth or to detect when the membranous labyrinth is in contact with the footplate of the stapes. Both tests may be administered with the manometer of an electroacoustic impedance instrument. When vertigo is a symptom, these tests should be a part of the routine impedance test battery.", "contents": "The fistula and Hennebert tests. The fistula and Hennebert tests are used to detect the structural integrity of the bony labyrinth or to detect when the membranous labyrinth is in contact with the footplate of the stapes. Both tests may be administered with the manometer of an electroacoustic impedance instrument. When vertigo is a symptom, these tests should be a part of the routine impedance test battery.", "PMID": 965273}
{"id": "pubmed23n0032_6237", "title": "Audiologic and metabolic findings in 90 patients with fluctuant hearing loss.", "content": "Fluctuant hearing loss is a common occurrence. It is difficult to diagnose in its early stages when hearing thresholds are near normal and the only complaints the patient has are of fullness and tinnitus. Audiologic tests are helpful in confirming the diagnosis. Impedance measurements are an accurate assessment of middle ear status and can assist in localizing the fullness experienced by these patients. Site of lesion tests and discrimination scores at various sensation levels are sensitive indexes of disease activity. Observations during medical treatment of 90 patients with metabolic dysfunction (hyperlipoproteinemia: hypoglycemia; hypothyroidism) suggest that discrimination scores fluctuate more widely than do pure tone thresholds over a period of time. Thirty patients were given complete audiologic testing after dietary management and treatment. All reported relief from tinnitus and fullness, and 15 or 50% showed improved audiograms and discrimination scores. Any change in the energy reserve or metabolic rate of the inner ear by a systemic metabolic dysfunction can contribute to or cause sensorineural hearing loss. Energy flow from metabolic sources is needed to transduce the acoustic stimuli into neural excitation patterns. The presence of any systemic metabolic dysfunction can be expected to contribute to and cause fluctuant hearing loss.", "contents": "Audiologic and metabolic findings in 90 patients with fluctuant hearing loss. Fluctuant hearing loss is a common occurrence. It is difficult to diagnose in its early stages when hearing thresholds are near normal and the only complaints the patient has are of fullness and tinnitus. Audiologic tests are helpful in confirming the diagnosis. Impedance measurements are an accurate assessment of middle ear status and can assist in localizing the fullness experienced by these patients. Site of lesion tests and discrimination scores at various sensation levels are sensitive indexes of disease activity. Observations during medical treatment of 90 patients with metabolic dysfunction (hyperlipoproteinemia: hypoglycemia; hypothyroidism) suggest that discrimination scores fluctuate more widely than do pure tone thresholds over a period of time. Thirty patients were given complete audiologic testing after dietary management and treatment. All reported relief from tinnitus and fullness, and 15 or 50% showed improved audiograms and discrimination scores. Any change in the energy reserve or metabolic rate of the inner ear by a systemic metabolic dysfunction can contribute to or cause sensorineural hearing loss. Energy flow from metabolic sources is needed to transduce the acoustic stimuli into neural excitation patterns. The presence of any systemic metabolic dysfunction can be expected to contribute to and cause fluctuant hearing loss.", "PMID": 965274}
{"id": "pubmed23n0032_6238", "title": "Dichotic listening in adults with sensorineural hearing loss.", "content": "Digits and consonant-vowel (CV) nonsense syllables were presented dichotically to 36 normal-hearing subjects and 36 subjects with bilaterally symmetrical sensorineural hearing loss. The normal-hearing subjects performed significantly better in the recall of both digits and CV nonsense syllables, and recall decreased significantly as the degree of hearing loss increased. The expected right ear advantage was observed for the normal-hearing subjects on both tests. As a group, the hearing-loss subjects showed no significant ear preference, and ear preference did not vary significantly with the degree of hearing loss. However, individual hearing-loss subjects showed marked ear asymmetry for the dichotic digits. For both dichotic tests, individual ear preference increased as the degree of hearing loss increased. Significant but low correlations were observed between better ear speech discrimination scores for the hearing-loss subjects and the preferred ear for dichotic CV nonsense syllables.", "contents": "Dichotic listening in adults with sensorineural hearing loss. Digits and consonant-vowel (CV) nonsense syllables were presented dichotically to 36 normal-hearing subjects and 36 subjects with bilaterally symmetrical sensorineural hearing loss. The normal-hearing subjects performed significantly better in the recall of both digits and CV nonsense syllables, and recall decreased significantly as the degree of hearing loss increased. The expected right ear advantage was observed for the normal-hearing subjects on both tests. As a group, the hearing-loss subjects showed no significant ear preference, and ear preference did not vary significantly with the degree of hearing loss. However, individual hearing-loss subjects showed marked ear asymmetry for the dichotic digits. For both dichotic tests, individual ear preference increased as the degree of hearing loss increased. Significant but low correlations were observed between better ear speech discrimination scores for the hearing-loss subjects and the preferred ear for dichotic CV nonsense syllables.", "PMID": 965275}
{"id": "pubmed23n0032_6239", "title": "Performance by cortical lesion patients on 40 and 60% time-compressed materials.", "content": "Tape recordings of time-compressed (40 and 60%) monosyllables were administered to 11 patients with diffuse unilateral temporal lobe lesion, 4 hemispherectomy patients, and 16 patients with discrete unilateral temporal lobe lesion. Time compression was accomplished with the Fairbanks electromechanical apparatus, which allowed temporal compression but did not introduce frequency distortion. The results revealed that with 60% time compression, all patients with diffuse unilateral cortical lesion showed breakdown of speech discrimination in the ear contralateral to the lesion. Patients with discrete unilateral cortical lesion generally did not demonstrate breakdown with the 60% time-compressed materials.", "contents": "Performance by cortical lesion patients on 40 and 60% time-compressed materials. Tape recordings of time-compressed (40 and 60%) monosyllables were administered to 11 patients with diffuse unilateral temporal lobe lesion, 4 hemispherectomy patients, and 16 patients with discrete unilateral temporal lobe lesion. Time compression was accomplished with the Fairbanks electromechanical apparatus, which allowed temporal compression but did not introduce frequency distortion. The results revealed that with 60% time compression, all patients with diffuse unilateral cortical lesion showed breakdown of speech discrimination in the ear contralateral to the lesion. Patients with discrete unilateral cortical lesion generally did not demonstrate breakdown with the 60% time-compressed materials.", "PMID": 965276}
{"id": "pubmed23n0032_6240", "title": "Granulomatous enteritis in nine horses.", "content": "Granulomatous enteritis was diagnosed in 9 horses between 1 and 11 years of age. The complaint in all cases was persistent weight loss. Four of the horses had chronic diarrhea. Two consistent diagnostic features were hypoalbuminemia and decreased phagocytic activity of mesothelial cells in the peritoneal fluid. In 2 cases the diagnosis was confirmed by biopsy of the rectal mucosa. Remaining cases were definitively diagnosed by exploratory laparotomy and biopsy or at necropsy.", "contents": "Granulomatous enteritis in nine horses. Granulomatous enteritis was diagnosed in 9 horses between 1 and 11 years of age. The complaint in all cases was persistent weight loss. Four of the horses had chronic diarrhea. Two consistent diagnostic features were hypoalbuminemia and decreased phagocytic activity of mesothelial cells in the peritoneal fluid. In 2 cases the diagnosis was confirmed by biopsy of the rectal mucosa. Remaining cases were definitively diagnosed by exploratory laparotomy and biopsy or at necropsy.", "PMID": 965279}
{"id": "pubmed23n0032_6241", "title": "Diagnosis and surgical treatment of cystlike lesions of the equine paranasal sinuses.", "content": "Development of cysts in the equine paranasal sinuses is probably a congenital disease; however, signs may not appear for several years. In 4 cases, clinical signs were observed when fluid accumulation within the cysts resulted in nasal discharge or facial swelling, or when secondary infection occurred. Treatment required surgical removal of the lining membrane and other involved tissues. Postoperative care included lavage of the sinuses and systemic antibiotic therapy.", "contents": "Diagnosis and surgical treatment of cystlike lesions of the equine paranasal sinuses. Development of cysts in the equine paranasal sinuses is probably a congenital disease; however, signs may not appear for several years. In 4 cases, clinical signs were observed when fluid accumulation within the cysts resulted in nasal discharge or facial swelling, or when secondary infection occurred. Treatment required surgical removal of the lining membrane and other involved tissues. Postoperative care included lavage of the sinuses and systemic antibiotic therapy.", "PMID": 965280}
{"id": "pubmed23n0032_6242", "title": "Temporal summation of the acoustic reflex in normal and sensorineural hearing-impaired ears.", "content": "Psychophysical and acoustic reflex temporal summation measures were obtained on normal-hearing and sensorineural hearing-impaired persons. Bursts of one-third octave noise with center frequencies of 500, 1000, 2000, and 4000 Hz and durations of 15, 20, 50, 100, 200, and 300 msec were used as stimuli. Reflexes were obtained using an acoustic impedance measuring technique. Results demonstrated that the duration of the noise stimuli had a profound effect on the acoustic reflex response and that the ear with M\u00e9ni\u00e8re's disease was clearly distinguishable from the normal ear by both psychophysical and acoustic reflex temporal summation measurements. The presbycusic subjects were separated from the normal subjects to a greater extent in the psychophysical data at the higher frequencies. The reflex thresholds for the noise bands and durations used in this study occurred at lower sound pressure levels than those previously obtained. The normal-hearing subjects failed to show any differences in either psychophysical or acoustic reflex temporal summation as a function of stimulus frequency.", "contents": "Temporal summation of the acoustic reflex in normal and sensorineural hearing-impaired ears. Psychophysical and acoustic reflex temporal summation measures were obtained on normal-hearing and sensorineural hearing-impaired persons. Bursts of one-third octave noise with center frequencies of 500, 1000, 2000, and 4000 Hz and durations of 15, 20, 50, 100, 200, and 300 msec were used as stimuli. Reflexes were obtained using an acoustic impedance measuring technique. Results demonstrated that the duration of the noise stimuli had a profound effect on the acoustic reflex response and that the ear with M\u00e9ni\u00e8re's disease was clearly distinguishable from the normal ear by both psychophysical and acoustic reflex temporal summation measurements. The presbycusic subjects were separated from the normal subjects to a greater extent in the psychophysical data at the higher frequencies. The reflex thresholds for the noise bands and durations used in this study occurred at lower sound pressure levels than those previously obtained. The normal-hearing subjects failed to show any differences in either psychophysical or acoustic reflex temporal summation as a function of stimulus frequency.", "PMID": 965277}
{"id": "pubmed23n0032_6243", "title": "Innervation of the equine hip and stifle joint capsules.", "content": "The hindlimbs of 3 ponies and 3 horses were dissected. The hip joint capsule was found to receive articular nerve fibers from the femoral, obturator, cranial gluteal, and sciatic nerves. The nerve fibers were distributed to the fibrous joint capsule and associated capsular ligaments. The stifle joint capsule was found to receive articular branches from the femoral, saphenous, obturator, common peroneal, and tibial nerves. The fibers terminated in the joint capsule, fat pad, patellar and collateral ligaments, and the internally situated meniscal and cruciate ligaments.", "contents": "Innervation of the equine hip and stifle joint capsules. The hindlimbs of 3 ponies and 3 horses were dissected. The hip joint capsule was found to receive articular nerve fibers from the femoral, obturator, cranial gluteal, and sciatic nerves. The nerve fibers were distributed to the fibrous joint capsule and associated capsular ligaments. The stifle joint capsule was found to receive articular branches from the femoral, saphenous, obturator, common peroneal, and tibial nerves. The fibers terminated in the joint capsule, fat pad, patellar and collateral ligaments, and the internally situated meniscal and cruciate ligaments.", "PMID": 965281}
{"id": "pubmed23n0032_6244", "title": "Sebaceous gland adenomas in dogs.", "content": "In a review of neoplasm registry records at Kansas State University (1961 through 1971), 162 sebaceous gland adenomas were reported in 31 breeds of dogs, representing 5.0% of 3,240 neoplasms recorded. Mean age of the affected dogs was 9.5 years. Females accounted for 56.1% of the recorded sebaceous gland adenomas. The tumors were removed surgically for biopsy in all dogs; 24 dogs had multiple concurrent skin neoplasms of a different cell type, and 7 dogs had recurrence of sebaceous gland adenomas, without metastases, and required further surgery. The skin of the hindquarters, abdomen, and thorax was a principal site (54%) of the sebaceous gland adenomas.", "contents": "Sebaceous gland adenomas in dogs. In a review of neoplasm registry records at Kansas State University (1961 through 1971), 162 sebaceous gland adenomas were reported in 31 breeds of dogs, representing 5.0% of 3,240 neoplasms recorded. Mean age of the affected dogs was 9.5 years. Females accounted for 56.1% of the recorded sebaceous gland adenomas. The tumors were removed surgically for biopsy in all dogs; 24 dogs had multiple concurrent skin neoplasms of a different cell type, and 7 dogs had recurrence of sebaceous gland adenomas, without metastases, and required further surgery. The skin of the hindquarters, abdomen, and thorax was a principal site (54%) of the sebaceous gland adenomas.", "PMID": 965289}
{"id": "pubmed23n0032_6245", "title": "Evaluation of a vaccine for control of streptococcic lymphadenitis of swine.", "content": "Three tirals were conducted to evaluate the use of a vaccine for control of streptococcic lymphadenitis of swine. Clinically normal swine were vaccinated orally, according to the manufacturer's instructions. Unvaccinated controls and the vaccinated pigs were subsequently inoculated with a virulent strain of the etiologic agent, group E Streptococcus, serotype IV. At necropsy, 47 of the 51 vaccinated pigs (92.1%) were free of cervical abscesses, whereas 20 of the 21 control pigs (95.2%) developed cervical abscesses.", "contents": "Evaluation of a vaccine for control of streptococcic lymphadenitis of swine. Three tirals were conducted to evaluate the use of a vaccine for control of streptococcic lymphadenitis of swine. Clinically normal swine were vaccinated orally, according to the manufacturer's instructions. Unvaccinated controls and the vaccinated pigs were subsequently inoculated with a virulent strain of the etiologic agent, group E Streptococcus, serotype IV. At necropsy, 47 of the 51 vaccinated pigs (92.1%) were free of cervical abscesses, whereas 20 of the 21 control pigs (95.2%) developed cervical abscesses.", "PMID": 965290}
{"id": "pubmed23n0032_6246", "title": "Porcine abortion caused by Actinobacillus equuli.", "content": "Actinobacillus equuli was isolated from an aborted porcine fetus. Fetal lesions of peracute embolic septicemia were similar to those seen in septicemic actinobacillosis of older swine and other species. This case represented an individual rather than a herd abortion problem.", "contents": "Porcine abortion caused by Actinobacillus equuli. Actinobacillus equuli was isolated from an aborted porcine fetus. Fetal lesions of peracute embolic septicemia were similar to those seen in septicemic actinobacillosis of older swine and other species. This case represented an individual rather than a herd abortion problem.", "PMID": 965291}
{"id": "pubmed23n0032_6247", "title": "Cranial cruciate ligament repair in a calf.", "content": "A 1-month-old calf was unable to use its right pelvic limb because of rupture of the cranial cruciate ligament. The ligament was replaced with a wedge of bone from the cranial one-third of the patella and the attached portion of the middle patellar tendon. Postoperative complications limited function of the joint.", "contents": "Cranial cruciate ligament repair in a calf. A 1-month-old calf was unable to use its right pelvic limb because of rupture of the cranial cruciate ligament. The ligament was replaced with a wedge of bone from the cranial one-third of the patella and the attached portion of the middle patellar tendon. Postoperative complications limited function of the joint.", "PMID": 965292}
{"id": "pubmed23n0032_6248", "title": "Effect of mechanical factors on the relation between rate and depth of breathing in cats.", "content": "In anesthetized tracheotomized cats we compared the time sequence of ventilatory events with neurological events (electrical activity of the diaphragm) during the breathing cycle. We found that under control conditions a phase shift existed between the neurological and the spirometric duration of inspiration, amounting to about 150 ms. This phase shift was increased by increasing the time constant of the respiratory system (resistive loading) and decreased by decreasing it (elastic loading). These phase shifts resulted in a difference in the appreciation of the volume responsible for the termination of inspiration (Hering-Breuer inhibition) because the volume corresponding to the end of the neurological event was somewhat smaller than the final tidal volume reached.", "contents": "Effect of mechanical factors on the relation between rate and depth of breathing in cats. In anesthetized tracheotomized cats we compared the time sequence of ventilatory events with neurological events (electrical activity of the diaphragm) during the breathing cycle. We found that under control conditions a phase shift existed between the neurological and the spirometric duration of inspiration, amounting to about 150 ms. This phase shift was increased by increasing the time constant of the respiratory system (resistive loading) and decreased by decreasing it (elastic loading). These phase shifts resulted in a difference in the appreciation of the volume responsible for the termination of inspiration (Hering-Breuer inhibition) because the volume corresponding to the end of the neurological event was somewhat smaller than the final tidal volume reached.", "PMID": 965293}
{"id": "pubmed23n0032_6249", "title": "Adaptation of anesthetized men to breathing through an inspiratory resistor.", "content": "Normal men anesthetized with methoxyflurane rebreathed carbon dioxide under two conditions. In one case they breathed most of the time through a low-resistance circuit and an inspiratory resistor of 40.4 cmH2O/1-s-1 was applied at intervals. In another case they breathed most of the time through the resistor and were allowed occasional free breaths. There were no differences between the two types of runs in tidal volume, respiratory frequency, duration of inspiration of loaded or unloaded breaths, or in amplitude or shape of occlusion pressure waves. It is concluded that the reaction of conscious men to an inspiratory resistive load, consisting of a compensatory augmentation of neural drive to respiratory muscles that does not depend on a chemical stimulus, is absent in anesthetized men.", "contents": "Adaptation of anesthetized men to breathing through an inspiratory resistor. Normal men anesthetized with methoxyflurane rebreathed carbon dioxide under two conditions. In one case they breathed most of the time through a low-resistance circuit and an inspiratory resistor of 40.4 cmH2O/1-s-1 was applied at intervals. In another case they breathed most of the time through the resistor and were allowed occasional free breaths. There were no differences between the two types of runs in tidal volume, respiratory frequency, duration of inspiration of loaded or unloaded breaths, or in amplitude or shape of occlusion pressure waves. It is concluded that the reaction of conscious men to an inspiratory resistive load, consisting of a compensatory augmentation of neural drive to respiratory muscles that does not depend on a chemical stimulus, is absent in anesthetized men.", "PMID": 965294}
{"id": "pubmed23n0032_6250", "title": "Physiological and behavioral reactions to repeated tail cooling in the white rat.", "content": "The purpose of the study was to examine a) the effect of local cooling on the metabolic rate, b) the effect of metabolic rate on the peripheral circulation, and c) acclimation to local cooling. After habituation to the experimental situation, 10 white rats had their tails exposed to ice water for 40 min/day for 37 days. Metabolic rate and tail temperature were measured. There was a conspicuous increase in oxygen consumption during the first 10 min of each cold exposure. This increase did not change significantly through the series. The metabolic rate during cooling was significantly reduced in the last half of the series. Tail temperature was significantly influenced by metabolic rate and increased significantly from the beginning of the series to the middle, and decreased from the middle to the end. Time of onset of the cold-induced vasodilatation decreased in the middle of the series and increased again at the end. These paradoxical findings are believed to be due to interplay between orienting reactions and habituation. It is pointed out that habituation may counteract acclimation.", "contents": "Physiological and behavioral reactions to repeated tail cooling in the white rat. The purpose of the study was to examine a) the effect of local cooling on the metabolic rate, b) the effect of metabolic rate on the peripheral circulation, and c) acclimation to local cooling. After habituation to the experimental situation, 10 white rats had their tails exposed to ice water for 40 min/day for 37 days. Metabolic rate and tail temperature were measured. There was a conspicuous increase in oxygen consumption during the first 10 min of each cold exposure. This increase did not change significantly through the series. The metabolic rate during cooling was significantly reduced in the last half of the series. Tail temperature was significantly influenced by metabolic rate and increased significantly from the beginning of the series to the middle, and decreased from the middle to the end. Time of onset of the cold-induced vasodilatation decreased in the middle of the series and increased again at the end. These paradoxical findings are believed to be due to interplay between orienting reactions and habituation. It is pointed out that habituation may counteract acclimation.", "PMID": 965295}
{"id": "pubmed23n0032_6251", "title": "Effects of intermittent hyperbaric oxygen on guinea pig lung elastin and collagen.", "content": "The effect of high oxygen pressure on collagen and elastin turnover in lung parenchyma tissue was studied in guinea pigs. Three groups of animals was used. One group (I) was given a clinically excessive exposure of 3 h daily for 9 days of 3 ATA oxygen. A second group (II) was given 3 ATA compressed air on the same schedule, and a third (III), a control, was given the same handling conditions only. Collagen and elastin which amounted to 10.45 and 4.62 mg, respectively, per 100 mg dry defatted parenchymal tissue was labeled with [14C]proline injected ip. Connective tissue protein turnover was estimated by labeling half of each group before exposure (a); effects on biosynthesis were determined by labeling the other half postexposure (b). No differences in hydroxyproline specific activity of collagen fractions or elastin were found with respect to either biosynthesis or turnover. Alveolar wall length-tension measurements were not changed. Since exposures were in excess of those used for treatment of gas gangrene, ischemic burns or wound healing, these results suggest that the metabolism of lung connective tissue is unaffected by the short-term exposure to hyperbaric oxygen.", "contents": "Effects of intermittent hyperbaric oxygen on guinea pig lung elastin and collagen. The effect of high oxygen pressure on collagen and elastin turnover in lung parenchyma tissue was studied in guinea pigs. Three groups of animals was used. One group (I) was given a clinically excessive exposure of 3 h daily for 9 days of 3 ATA oxygen. A second group (II) was given 3 ATA compressed air on the same schedule, and a third (III), a control, was given the same handling conditions only. Collagen and elastin which amounted to 10.45 and 4.62 mg, respectively, per 100 mg dry defatted parenchymal tissue was labeled with [14C]proline injected ip. Connective tissue protein turnover was estimated by labeling half of each group before exposure (a); effects on biosynthesis were determined by labeling the other half postexposure (b). No differences in hydroxyproline specific activity of collagen fractions or elastin were found with respect to either biosynthesis or turnover. Alveolar wall length-tension measurements were not changed. Since exposures were in excess of those used for treatment of gas gangrene, ischemic burns or wound healing, these results suggest that the metabolism of lung connective tissue is unaffected by the short-term exposure to hyperbaric oxygen.", "PMID": 965296}
{"id": "pubmed23n0032_6252", "title": "Gas-blood CO2 equilibration in parabronchial lungs of birds.", "content": "We have conducted two experimental series in the chicken in order to study CO2 exchange in the parabronchial lungs of birds. In the first series, the animals were artifically ventilated and end-expired PCO2, PE'CO2, was measured and compared with mixed venous PCO2, PVCO2. On the average, PECO2 exceeded PVCO2 by 2.8 Torr. In the second series, rebreathing was used to investigate the mechanism of this positive (PE'-PV)CO2 difference. Lung gas PCO2 was found to equilibrate with PVCO2 if both CO2 and O2 exchange in the lung was abolished during rebreathing. Only if O2 uptake continued, we observed a positive gas-to-mixed venous blood PCO2 difference. The results suggest that positive gas-blood PCO2 differences both during rebreathing and steady-state ventilation are brought about by the Haldane effect. Model calculations show that in the homogeneous avian lung, unlike in the alveolar lung, the Haldane effect can produce positive (PE'-PV)CO2 differences during steady-state breathing due to the peculiarities of the crosscurrent arrangement and parabronchial ventilation and blood perfusion.", "contents": "Gas-blood CO2 equilibration in parabronchial lungs of birds. We have conducted two experimental series in the chicken in order to study CO2 exchange in the parabronchial lungs of birds. In the first series, the animals were artifically ventilated and end-expired PCO2, PE'CO2, was measured and compared with mixed venous PCO2, PVCO2. On the average, PECO2 exceeded PVCO2 by 2.8 Torr. In the second series, rebreathing was used to investigate the mechanism of this positive (PE'-PV)CO2 difference. Lung gas PCO2 was found to equilibrate with PVCO2 if both CO2 and O2 exchange in the lung was abolished during rebreathing. Only if O2 uptake continued, we observed a positive gas-to-mixed venous blood PCO2 difference. The results suggest that positive gas-blood PCO2 differences both during rebreathing and steady-state ventilation are brought about by the Haldane effect. Model calculations show that in the homogeneous avian lung, unlike in the alveolar lung, the Haldane effect can produce positive (PE'-PV)CO2 differences during steady-state breathing due to the peculiarities of the crosscurrent arrangement and parabronchial ventilation and blood perfusion.", "PMID": 965297}
{"id": "pubmed23n0032_6253", "title": "Uterine work in parturition.", "content": "This  paper presents a theoretical analysis relating work expended by the uterus to the synergistic relationship between intrauterine pressure (IUP) and cervical dilatation (CD). By utilizing a geometrical model for the fetal presenting part which is assumed to be in contact with the cervix, the changes in internal volume of the uterus with each contraction can be shown to be functionally related to cervical dilatation. Thus work, expressed in terms of foot-pounds, can be calculated using the integral of pressure times incremental volume. By simulating intrauterine pressure and cervical dilatation with continuous analytical wave forms, the alterations required in uterine work to dilate the cervix are calculated for various wave-form aberrations which are seen in clinical situations. The wave-form aberrations are applied to an elastic cervical model as well as a viscoelastic model. Using the principles of thermodynamics the areas of the fetouterine complex which absorb the work generated by the contractions are defined. It is shown that the efficiency of the contractions to dilate the cervix can be calculated by evaluating the work expended in these various areas of energy absorption. The purpose of this paper is to present the theory upon which clinical findings in obstetrics can be based so that conclusions drawn will be technically sound.", "contents": "Uterine work in parturition. This  paper presents a theoretical analysis relating work expended by the uterus to the synergistic relationship between intrauterine pressure (IUP) and cervical dilatation (CD). By utilizing a geometrical model for the fetal presenting part which is assumed to be in contact with the cervix, the changes in internal volume of the uterus with each contraction can be shown to be functionally related to cervical dilatation. Thus work, expressed in terms of foot-pounds, can be calculated using the integral of pressure times incremental volume. By simulating intrauterine pressure and cervical dilatation with continuous analytical wave forms, the alterations required in uterine work to dilate the cervix are calculated for various wave-form aberrations which are seen in clinical situations. The wave-form aberrations are applied to an elastic cervical model as well as a viscoelastic model. Using the principles of thermodynamics the areas of the fetouterine complex which absorb the work generated by the contractions are defined. It is shown that the efficiency of the contractions to dilate the cervix can be calculated by evaluating the work expended in these various areas of energy absorption. The purpose of this paper is to present the theory upon which clinical findings in obstetrics can be based so that conclusions drawn will be technically sound.", "PMID": 965298}
{"id": "pubmed23n0032_6254", "title": "Plasma renin activity, angiotensin II, and aldosterone during intense heat stress.", "content": "Plasma renin activity (PRA), angiotensin II, and aldosterone levels, arterial blood pressure, and heart rate of six male students were investigated during and after heat stress in a sauna bath. Increased PRA, angiotensin II, and aldosterone levels were found both during and after sauna. The greatest mean increases in PRA (94.9 +/- 10.4% SE, P less than 0.005) and angiotensin II (196 +/- 54.7% SE, P less than 0.02) were observed at the end of the heat stress (at 20 min), and that in plasma aldosterone (505 +/- 209% SE, P less than 0.02) 30 min after the sauna. The heart rate roughly doubled during the heat stress and there was a transient increase followed by a decrease in systolic blood pressure and a decrease in diastolic blood pressure. This study demonstrates that intense heat stress can cause remarkable changes in the three main components of the renin-angiotensin-aldosterone system.", "contents": "Plasma renin activity, angiotensin II, and aldosterone during intense heat stress. Plasma renin activity (PRA), angiotensin II, and aldosterone levels, arterial blood pressure, and heart rate of six male students were investigated during and after heat stress in a sauna bath. Increased PRA, angiotensin II, and aldosterone levels were found both during and after sauna. The greatest mean increases in PRA (94.9 +/- 10.4% SE, P less than 0.005) and angiotensin II (196 +/- 54.7% SE, P less than 0.02) were observed at the end of the heat stress (at 20 min), and that in plasma aldosterone (505 +/- 209% SE, P less than 0.02) 30 min after the sauna. The heart rate roughly doubled during the heat stress and there was a transient increase followed by a decrease in systolic blood pressure and a decrease in diastolic blood pressure. This study demonstrates that intense heat stress can cause remarkable changes in the three main components of the renin-angiotensin-aldosterone system.", "PMID": 965299}
{"id": "pubmed23n0032_6255", "title": "Evaluation of the isolated paced rat heart.", "content": "Ventricular performance and coronary flow in Langendorff perfused rat hearts were measured over a wide range of perfusion pressures and heart rates. A change in aortic pressure from 60 to 120 mmHg induced a linear increase in coronary flow, ventricular systolic pressure, and contractility. Ventricular pacing from 300 to 600 beats/min under a constant afterload had no effect on coronary flow. Systolic pressure remained stable up to 400-450 beats/min and then decreased 14% at 600 beats/min compared to the nonpaced controls. When contraction rate exceeded 450 beats/min diastolic pressure progressively increased as the heart rate was elevated. Contractility decreased rapidly between 450 and 600 beats/min under all perfusion pressures. These data indicate that this heart model is physiologically stable with heart rates less than 450 beats/min and may be useful in studying tachycardia-induced work overload.", "contents": "Evaluation of the isolated paced rat heart. Ventricular performance and coronary flow in Langendorff perfused rat hearts were measured over a wide range of perfusion pressures and heart rates. A change in aortic pressure from 60 to 120 mmHg induced a linear increase in coronary flow, ventricular systolic pressure, and contractility. Ventricular pacing from 300 to 600 beats/min under a constant afterload had no effect on coronary flow. Systolic pressure remained stable up to 400-450 beats/min and then decreased 14% at 600 beats/min compared to the nonpaced controls. When contraction rate exceeded 450 beats/min diastolic pressure progressively increased as the heart rate was elevated. Contractility decreased rapidly between 450 and 600 beats/min under all perfusion pressures. These data indicate that this heart model is physiologically stable with heart rates less than 450 beats/min and may be useful in studying tachycardia-induced work overload.", "PMID": 965300}
{"id": "pubmed23n0032_6256", "title": "Exercise stress and enzyme-induced emphysema.", "content": "Rats were exposed to an aerosol of 10% papain for 8 h found in pilot studies to produce marked emphysema. One week after exposure some animals were forced to exercise in a motor-driven activity wheel 2 h daily for 4 wk, while others remained at rest. Another group of rats which served as the control were exposed to saline under the same conditions and divided into exercise and rest groups. Elastic recoil pressure (Pst) of lung was measured from static deflation pressure-volume curves with air and saline. Pst measured in air-filled lungs was not significantly different between the emphysema-rest and emphysema-exercise groups. When inflated with saline the Pst was significantly reduced in the emphysema-exercise compared to the emphysema-rest group at high and mid (100, 80, 60%) lung volumes. Pst in air- and saline-filled lungs was not significantly different between the control-rest and control-exercise groups. We therefore conclude that mechanical stress resulting from physical exercise decreased Pst of lung tissue in emphysematous rats.", "contents": "Exercise stress and enzyme-induced emphysema. Rats were exposed to an aerosol of 10% papain for 8 h found in pilot studies to produce marked emphysema. One week after exposure some animals were forced to exercise in a motor-driven activity wheel 2 h daily for 4 wk, while others remained at rest. Another group of rats which served as the control were exposed to saline under the same conditions and divided into exercise and rest groups. Elastic recoil pressure (Pst) of lung was measured from static deflation pressure-volume curves with air and saline. Pst measured in air-filled lungs was not significantly different between the emphysema-rest and emphysema-exercise groups. When inflated with saline the Pst was significantly reduced in the emphysema-exercise compared to the emphysema-rest group at high and mid (100, 80, 60%) lung volumes. Pst in air- and saline-filled lungs was not significantly different between the control-rest and control-exercise groups. We therefore conclude that mechanical stress resulting from physical exercise decreased Pst of lung tissue in emphysematous rats.", "PMID": 965301}
{"id": "pubmed23n0032_6257", "title": "Role of the carotid bodies in the heart rate response to breath holding in man.", "content": "To investigate the role of the carotid bodies in regulating the bradycardia of breath holding in man, we studied heart rate (HR) responses to prolonged breath holding (BH) in five asymptomatic asthmatic patients whose carotid bodies had been resected (CBR). Seven normal subjects served as controls. BH experiments were randomly initiated with single breaths of 100%, 21%, or 12% 92. During BH with 21% O2, normal subjects displayed the typical bradycardia; this response, however, was attenuated with the other O2 concentrations. In contrast, the CBR subjects manifested BH tachycardia which was inversely proportional to the O2 tension. HR increased in be CBR group by 5%, 31%, and 45% during BH with 100%, 21%, and 12% O2, respectively. These results demonstrate that the bradycardia of BH in normal man is under the influence of the carotid bodies. During BH and in the absence of carotid bodies, an O2 tension-dependent tachycardia is unveiled.", "contents": "Role of the carotid bodies in the heart rate response to breath holding in man. To investigate the role of the carotid bodies in regulating the bradycardia of breath holding in man, we studied heart rate (HR) responses to prolonged breath holding (BH) in five asymptomatic asthmatic patients whose carotid bodies had been resected (CBR). Seven normal subjects served as controls. BH experiments were randomly initiated with single breaths of 100%, 21%, or 12% 92. During BH with 21% O2, normal subjects displayed the typical bradycardia; this response, however, was attenuated with the other O2 concentrations. In contrast, the CBR subjects manifested BH tachycardia which was inversely proportional to the O2 tension. HR increased in be CBR group by 5%, 31%, and 45% during BH with 100%, 21%, and 12% O2, respectively. These results demonstrate that the bradycardia of BH in normal man is under the influence of the carotid bodies. During BH and in the absence of carotid bodies, an O2 tension-dependent tachycardia is unveiled.", "PMID": 965302}
{"id": "pubmed23n0032_6258", "title": "Decompression-induced decrease in nitrogen elimination rate in awake dogs.", "content": "Formulation of safe decompression procedures still requires unproven assumptions regarding both gas equilibration rates and the associated ascent criteria. Although the assumption of symmetry of uptake and elimination rates has been suspect for several years, few data are available. Measurements of actual mixed venous blood nitrogen content [vN2] during compression and following decompression in chronically catheterized awake dogs have clearly demonstrated that desaturation is markedly slower than saturation, and that this effect can be imposed by decompression. The disappearance of arteriovenous nitrogen concentration differences during desaturation following a decompression that produced decompression sickness indicates that cardiopulmonary and cardiovascular changes induced by mechanisms associated with decompression per se can potentiate its deleterious effects. Current US practices do not provide for such asymmetry, while those used in the UK have incorporated this in their models for the last decade.", "contents": "Decompression-induced decrease in nitrogen elimination rate in awake dogs. Formulation of safe decompression procedures still requires unproven assumptions regarding both gas equilibration rates and the associated ascent criteria. Although the assumption of symmetry of uptake and elimination rates has been suspect for several years, few data are available. Measurements of actual mixed venous blood nitrogen content [vN2] during compression and following decompression in chronically catheterized awake dogs have clearly demonstrated that desaturation is markedly slower than saturation, and that this effect can be imposed by decompression. The disappearance of arteriovenous nitrogen concentration differences during desaturation following a decompression that produced decompression sickness indicates that cardiopulmonary and cardiovascular changes induced by mechanisms associated with decompression per se can potentiate its deleterious effects. Current US practices do not provide for such asymmetry, while those used in the UK have incorporated this in their models for the last decade.", "PMID": 965303}
{"id": "pubmed23n0032_6259", "title": "Depressed myocardial function in the goat at high altitude.", "content": "To determine if depressed myocardial function contributes to the reported decrease in cardiac performance at high altitude, six chronically instrumented, unsedated goats were studied before, during, and after 2-wk exposure to hypobaric hypoxia (PaO2 44 mmHg). Undistorted ventricular pressure wave form was obtained from a miniature transducer implanted in the left ventricular cavity. The relationship between (dP/dt)/28P and P was extrapolated to obtain Vmax as an index of myocardial function. With beta sympathetic blockade (practolol) and pacing to reproduce heart rates, Vmax was uniformly and significantly depressed (P less than 0.01) during chronic hypoxia, and returned to control values following descent to low altitude. Likewise, stroke volume following saline infusion was decreased at high altitude and returned to control values following descent. Acute relief of hypoxia at high altitude by administration of 100% oxygen by mask did not reverse the depressed Vmax. These findings indicate that chronic hypobaric hypoxia produces a depression of myocardial function which is reversible by chronic but not acute relief of hypoxia.", "contents": "Depressed myocardial function in the goat at high altitude. To determine if depressed myocardial function contributes to the reported decrease in cardiac performance at high altitude, six chronically instrumented, unsedated goats were studied before, during, and after 2-wk exposure to hypobaric hypoxia (PaO2 44 mmHg). Undistorted ventricular pressure wave form was obtained from a miniature transducer implanted in the left ventricular cavity. The relationship between (dP/dt)/28P and P was extrapolated to obtain Vmax as an index of myocardial function. With beta sympathetic blockade (practolol) and pacing to reproduce heart rates, Vmax was uniformly and significantly depressed (P less than 0.01) during chronic hypoxia, and returned to control values following descent to low altitude. Likewise, stroke volume following saline infusion was decreased at high altitude and returned to control values following descent. Acute relief of hypoxia at high altitude by administration of 100% oxygen by mask did not reverse the depressed Vmax. These findings indicate that chronic hypobaric hypoxia produces a depression of myocardial function which is reversible by chronic but not acute relief of hypoxia.", "PMID": 965304}
{"id": "pubmed23n0032_6260", "title": "Myocardial interaction between the ventricles.", "content": "The myocardial interaction between the ventricles was studied using isolated, flow-perfused, paced rabbit hearts beating isovolumically. In general, increasing left ventricular (LV) volume increased right ventricular (RV) diastolic and developed pressures. In particular, with a peak RV volume (RVV), increasing LV volume (LVV) from zero to two-thirds of its peak volume increase RV diastolic pressure by 1.7 mmHg (N=10, P less than 0.001) and RV developed pressure by 1.5 mmHg (N=10,P less than 0.001). For the LV, small RVV caused LV diastolic and developed pressure to increase, while large RVV increased LV diastolic pressure but decreased LV developed pressure. With a LVV held at two-third of peak volume, increasing RVV from zero to its peak volume caused LV diastolic pressure to increase by 2.5 mmHg (N=10,P less than 0.001) and LV developed pressure to decrease by 2.0 mmHg (N=10, P less than 0.001). The position of the interventricular septum correlated with LV diastolic pressure and RV diastolic and developed pressure changes (P less than 0.01). The results demonstrate that the diastolic and developed pressure-volume relationships of either ventricle can be acutely altered by varying the volume of the other ventricle.", "contents": "Myocardial interaction between the ventricles. The myocardial interaction between the ventricles was studied using isolated, flow-perfused, paced rabbit hearts beating isovolumically. In general, increasing left ventricular (LV) volume increased right ventricular (RV) diastolic and developed pressures. In particular, with a peak RV volume (RVV), increasing LV volume (LVV) from zero to two-thirds of its peak volume increase RV diastolic pressure by 1.7 mmHg (N=10, P less than 0.001) and RV developed pressure by 1.5 mmHg (N=10,P less than 0.001). For the LV, small RVV caused LV diastolic and developed pressure to increase, while large RVV increased LV diastolic pressure but decreased LV developed pressure. With a LVV held at two-third of peak volume, increasing RVV from zero to its peak volume caused LV diastolic pressure to increase by 2.5 mmHg (N=10,P less than 0.001) and LV developed pressure to decrease by 2.0 mmHg (N=10, P less than 0.001). The position of the interventricular septum correlated with LV diastolic pressure and RV diastolic and developed pressure changes (P less than 0.01). The results demonstrate that the diastolic and developed pressure-volume relationships of either ventricle can be acutely altered by varying the volume of the other ventricle.", "PMID": 965305}
{"id": "pubmed23n0032_6261", "title": "Mechanics of the canine diaphragm.", "content": "Diaphragmatic force-length behavior was assessed in anesthetized dogs using two techniques. One employed measurements in a diaphragm strip with intact nerve and blood supply; the second related transdiaphragmatic pressure (Pdi) to direct estimates of diaphragmatic tension from strain gauge arches during bilateral, tetanic, supramaximal phrenic nerve stimulation. In strip preparations the diaphragm exerted active force at lengths as short as 40% of resting in situ length (Lo), and maximal force was registered at a length averaging 25% greater than Lo. This suggested a broader effective length range for the diaphragm as compared to other skeletal muscles. In the intact preparation both Pdi and directly measured diaphragmatic tension were inversely related to lung volume and when they were used to calculate the diaphragm's effective radius of curvature (r), r was found to change little or decrease at large lung volumes rather than increase as theory would predict. These findings suggest that length tension characteristics outweigh geometric considerations in explaining the diaphragm's function in normal dogs and probably normal men.", "contents": "Mechanics of the canine diaphragm. Diaphragmatic force-length behavior was assessed in anesthetized dogs using two techniques. One employed measurements in a diaphragm strip with intact nerve and blood supply; the second related transdiaphragmatic pressure (Pdi) to direct estimates of diaphragmatic tension from strain gauge arches during bilateral, tetanic, supramaximal phrenic nerve stimulation. In strip preparations the diaphragm exerted active force at lengths as short as 40% of resting in situ length (Lo), and maximal force was registered at a length averaging 25% greater than Lo. This suggested a broader effective length range for the diaphragm as compared to other skeletal muscles. In the intact preparation both Pdi and directly measured diaphragmatic tension were inversely related to lung volume and when they were used to calculate the diaphragm's effective radius of curvature (r), r was found to change little or decrease at large lung volumes rather than increase as theory would predict. These findings suggest that length tension characteristics outweigh geometric considerations in explaining the diaphragm's function in normal dogs and probably normal men.", "PMID": 965306}
{"id": "pubmed23n0032_6262", "title": "Modulation of carotid baroreflex responsiveness in man: effects of posture and propranolol.", "content": "Carotid baroreceptors were stimulated with graded neck suction in supine and standing volunteers, before and after autonomic blockade, to determine the influence of posture on baroreflex responsiveness. Propranolol significantly augmented baroreflex pulse interval prolongation in the supine position. Upright posture did not modify baroreflex pulse interval responses prior to propranolol, but significantly augmented responses after propranolol. The results suggest that standing enhances baroreflex sensitivity, but that under normal circumstances, this effect is masked by beta-adrenergic stimulation. Augmentation of baroreflex pulse interval prolongation in the supine and standing positions by propranolol may contribute to the effectiveness of this drug in angina pectoris and labile hypertension.", "contents": "Modulation of carotid baroreflex responsiveness in man: effects of posture and propranolol. Carotid baroreceptors were stimulated with graded neck suction in supine and standing volunteers, before and after autonomic blockade, to determine the influence of posture on baroreflex responsiveness. Propranolol significantly augmented baroreflex pulse interval prolongation in the supine position. Upright posture did not modify baroreflex pulse interval responses prior to propranolol, but significantly augmented responses after propranolol. The results suggest that standing enhances baroreflex sensitivity, but that under normal circumstances, this effect is masked by beta-adrenergic stimulation. Augmentation of baroreflex pulse interval prolongation in the supine and standing positions by propranolol may contribute to the effectiveness of this drug in angina pectoris and labile hypertension.", "PMID": 965307}
{"id": "pubmed23n0032_6263", "title": "Distribution of pulmonary resistance: effects of gas density, viscosity, and flow rate.", "content": "Theoretical predictions of the distribution of inspiratory viscous pressure loss were made for canine and human pulmonary airways for gases with varying density and viscosity at different pulmonary flow rates. We predicted that in canine or human airways when tracheal flow was turbulent, most of the total calculated pressure loss would be in the first few branchings of the bronchial tree; however when tracheal flow was nearly laminar inspiratory pressure loss would be spread more uniformly along the airways. To test these predictions an airway catheter was used to partition total pulmonary resistance (RL) in five anesthetized dogs. On air the catheter was positioned such that the mean resistance mouthward of the catheter tip (Rc) at a flow of 0.5 1/s was 63% of RL. At the same catheter position Rc was 87% of RL when the dogs were breathing a mixture of 80% sulfur hexafluoride-20% oxygen and resistance was determined at 1.0 1/s. Rc was 50% of RL when the dogs were breathing a mixture of 80% helium-20% oxygen and resistance was determined at 0.25 1/s. Thus altering gas physical properties and flow rates changed the distribution of pulmonary resistance as predicted.", "contents": "Distribution of pulmonary resistance: effects of gas density, viscosity, and flow rate. Theoretical predictions of the distribution of inspiratory viscous pressure loss were made for canine and human pulmonary airways for gases with varying density and viscosity at different pulmonary flow rates. We predicted that in canine or human airways when tracheal flow was turbulent, most of the total calculated pressure loss would be in the first few branchings of the bronchial tree; however when tracheal flow was nearly laminar inspiratory pressure loss would be spread more uniformly along the airways. To test these predictions an airway catheter was used to partition total pulmonary resistance (RL) in five anesthetized dogs. On air the catheter was positioned such that the mean resistance mouthward of the catheter tip (Rc) at a flow of 0.5 1/s was 63% of RL. At the same catheter position Rc was 87% of RL when the dogs were breathing a mixture of 80% sulfur hexafluoride-20% oxygen and resistance was determined at 1.0 1/s. Rc was 50% of RL when the dogs were breathing a mixture of 80% helium-20% oxygen and resistance was determined at 0.25 1/s. Thus altering gas physical properties and flow rates changed the distribution of pulmonary resistance as predicted.", "PMID": 965308}
{"id": "pubmed23n0032_6264", "title": "Localization of airway constriction using gases of varying density and viscosity.", "content": "The relationship between the major site of airway constriction and change in total pulmonary resistance while breathing gases of varying density and viscosity was studied in five anesthetized dogs pretreated with atropine. Using an airway catheter, central and peripheral components of pulmonary resistance were measured by forced oscillation. Total pulmonary resistance was measured at 0.5 1/s with lungs air-filled, at 0.25 1/s with the lungs filled with 80% helium-20% oxygen (RL-He), and at 1.0 1/s with 80% sulfur hexafluoride-20% oxygen (RL-SF6). Intravenous histamine infusion resulted in a predominantly peripheral resistance increase as determined by the airway catheter and a much larger percentage increase in RL-He than in RL-SF6. Tracheal banding produced a purely central resistance increase and a greater change in RL-SF6 than in FL-He. These results support theoretical predictions that the predominant site of airways constriction can be determined without on airway catheter by comparing relative changes in total pulmonary resistance using different flow regimes.", "contents": "Localization of airway constriction using gases of varying density and viscosity. The relationship between the major site of airway constriction and change in total pulmonary resistance while breathing gases of varying density and viscosity was studied in five anesthetized dogs pretreated with atropine. Using an airway catheter, central and peripheral components of pulmonary resistance were measured by forced oscillation. Total pulmonary resistance was measured at 0.5 1/s with lungs air-filled, at 0.25 1/s with the lungs filled with 80% helium-20% oxygen (RL-He), and at 1.0 1/s with 80% sulfur hexafluoride-20% oxygen (RL-SF6). Intravenous histamine infusion resulted in a predominantly peripheral resistance increase as determined by the airway catheter and a much larger percentage increase in RL-He than in RL-SF6. Tracheal banding produced a purely central resistance increase and a greater change in RL-SF6 than in FL-He. These results support theoretical predictions that the predominant site of airways constriction can be determined without on airway catheter by comparing relative changes in total pulmonary resistance using different flow regimes.", "PMID": 965309}
{"id": "pubmed23n0032_6265", "title": "Determinants of regional distribution of a bolus inhaled from residual volume.", "content": "The volume of lung at residual volume (RV) which fails to receive an inhaled tracer bolus (EXV) was quantitated in 13 normals by comparison of a scintigram of the distribution of a tracer bolus inhaled from RV (BORV) with a scintigram at RV of lung equilibrated with the tracer (EQRV). EXV was found in the dependent lung in the erect position in all subjects but also occurred to a lesser degree at the apex in 11 of 13 subjects. Basal EXV ranged from 1 to 7% of TLC, and unlike apical EXV increased with age (r= 0.91, P less than 0.01). EXV in the decubitus position shifted largely to the dependent lung with none remaining in the original erect apical and basal locations, demonstrating that gravity determined EXV location. Nitrous oxide, which is highly diffusible, failed in four subjects to carry the tracer to basal EXV even though perfusion was documented to persist to this area, implying basal EXV airways were closed, not narrowed. In one of the four subjects apical EXV was readily definable. Nitrous oxide carried tracer into this region, implying patent apical EXV airways.", "contents": "Determinants of regional distribution of a bolus inhaled from residual volume. The volume of lung at residual volume (RV) which fails to receive an inhaled tracer bolus (EXV) was quantitated in 13 normals by comparison of a scintigram of the distribution of a tracer bolus inhaled from RV (BORV) with a scintigram at RV of lung equilibrated with the tracer (EQRV). EXV was found in the dependent lung in the erect position in all subjects but also occurred to a lesser degree at the apex in 11 of 13 subjects. Basal EXV ranged from 1 to 7% of TLC, and unlike apical EXV increased with age (r= 0.91, P less than 0.01). EXV in the decubitus position shifted largely to the dependent lung with none remaining in the original erect apical and basal locations, demonstrating that gravity determined EXV location. Nitrous oxide, which is highly diffusible, failed in four subjects to carry the tracer to basal EXV even though perfusion was documented to persist to this area, implying basal EXV airways were closed, not narrowed. In one of the four subjects apical EXV was readily definable. Nitrous oxide carried tracer into this region, implying patent apical EXV airways.", "PMID": 965310}
{"id": "pubmed23n0032_6266", "title": "Storage telemetric system for detection of radionuclide tracers in humans.", "content": "An external-storage telemetric device for continuous detection and registration of radionuclide tracers in humans is described. The weight of the system is 600 g. A pocket-sized ECG cassette tape recorder with 24-h continuous registration constitutes the storage unit. Two end-window GM detectors (Philips 18515) are used as surface detectors. Both gamma- and beta-emitters are detected by this equipment. The linear range is 100 counts/s. Renal excretion of [125I]iodide during 30 h in a normal person is shown. The disappearance rates are 0.1 h-1 during the day and 0.05 h-1 during the night. The coefficient of variation of the individual slopes has been calculated to less than 5%. A comparison with the analog radiotelemetric equipment is shown. The storage telemetric equipment can be applied outside the hospital, when the patient is ambulatory.", "contents": "Storage telemetric system for detection of radionuclide tracers in humans. An external-storage telemetric device for continuous detection and registration of radionuclide tracers in humans is described. The weight of the system is 600 g. A pocket-sized ECG cassette tape recorder with 24-h continuous registration constitutes the storage unit. Two end-window GM detectors (Philips 18515) are used as surface detectors. Both gamma- and beta-emitters are detected by this equipment. The linear range is 100 counts/s. Renal excretion of [125I]iodide during 30 h in a normal person is shown. The disappearance rates are 0.1 h-1 during the day and 0.05 h-1 during the night. The coefficient of variation of the individual slopes has been calculated to less than 5%. A comparison with the analog radiotelemetric equipment is shown. The storage telemetric equipment can be applied outside the hospital, when the patient is ambulatory.", "PMID": 965311}
{"id": "pubmed23n0032_6267", "title": "Ventilation-perfusion ratio obtained by a noninvasive frequency response technique.", "content": "Results of animal experiments using sinusoidal changes in inspired halothane concentration showed that the ratio of variation in end-expired concentration to the variation in inspired concentration reached a plateau in the Bode diagram. With the help of an uptake and distribution model, the interpretation of the results showed that the level of the plateau is determined by the overall ventilation-perfusion ratio. With a good selection of input frequency, tracer agent, and known ventilation, the ventilation-perfusion ratio and the lung perfusion can be consequently obtained noninvasively. Mean ventilation-perfusion ratio was determined with 20 human voluteers. At rest a mean ratio was found of 0.87 +/- 0.28 (SD). At a work load of 90 W a mean ratio was found of 1.19 +/- 0.19 (SD). In two individuals reproducibility and influence of CO2 was studied. At rest without additional CO2 the ventilation-perfusion ratio was 0.71 +/- 0.06 (SD) obtained with a constant breathing rate of 10/min. At an end-expired CO2 level of 6% the ventilation-perfusion ratio was increased almost 2.5 times. The calculated perfusion with and without increased end-expired CO2 levels under the same work load were well reproducible.", "contents": "Ventilation-perfusion ratio obtained by a noninvasive frequency response technique. Results of animal experiments using sinusoidal changes in inspired halothane concentration showed that the ratio of variation in end-expired concentration to the variation in inspired concentration reached a plateau in the Bode diagram. With the help of an uptake and distribution model, the interpretation of the results showed that the level of the plateau is determined by the overall ventilation-perfusion ratio. With a good selection of input frequency, tracer agent, and known ventilation, the ventilation-perfusion ratio and the lung perfusion can be consequently obtained noninvasively. Mean ventilation-perfusion ratio was determined with 20 human voluteers. At rest a mean ratio was found of 0.87 +/- 0.28 (SD). At a work load of 90 W a mean ratio was found of 1.19 +/- 0.19 (SD). In two individuals reproducibility and influence of CO2 was studied. At rest without additional CO2 the ventilation-perfusion ratio was 0.71 +/- 0.06 (SD) obtained with a constant breathing rate of 10/min. At an end-expired CO2 level of 6% the ventilation-perfusion ratio was increased almost 2.5 times. The calculated perfusion with and without increased end-expired CO2 levels under the same work load were well reproducible.", "PMID": 965312}
{"id": "pubmed23n0032_6268", "title": "Novel telemetric system for extraluminal measurement of gastrointestinal motility.", "content": "A frequency-modulated, telemetric system with a transducer sensitive to variation in distance between two points was developed and used to measure in vivo small intestinal motor function in unanesthetized dogs. The construction of the system and one application of it to the study of gastrointestinal motility is described. Data were recorded after fasting, 1 h after feeding a standardized meat meal, and after intravenous injections of pentagastrin. A significant increase in motility was seen after pentagastrin injections and feeding as compared to the motility pattern seen in a fasting animal, and the telemetric system was proven adequate for in vivo measurement of motility of the gastrointestinal tract.", "contents": "Novel telemetric system for extraluminal measurement of gastrointestinal motility. A frequency-modulated, telemetric system with a transducer sensitive to variation in distance between two points was developed and used to measure in vivo small intestinal motor function in unanesthetized dogs. The construction of the system and one application of it to the study of gastrointestinal motility is described. Data were recorded after fasting, 1 h after feeding a standardized meat meal, and after intravenous injections of pentagastrin. A significant increase in motility was seen after pentagastrin injections and feeding as compared to the motility pattern seen in a fasting animal, and the telemetric system was proven adequate for in vivo measurement of motility of the gastrointestinal tract.", "PMID": 965313}
{"id": "pubmed23n0032_6269", "title": "Small inexpensive anesthetic apparatus for rats.", "content": "An anesthetic apparatus is described that is capable of delivering easily controlled, constant ether-air mixtures to rats and other small laboratory animals. The apparatus is readily calibrated and is stable for extended periods of time under laboratory conditions. In  addition, the apparatus can be constructed of commonly available components.", "contents": "Small inexpensive anesthetic apparatus for rats. An anesthetic apparatus is described that is capable of delivering easily controlled, constant ether-air mixtures to rats and other small laboratory animals. The apparatus is readily calibrated and is stable for extended periods of time under laboratory conditions. In  addition, the apparatus can be constructed of commonly available components.", "PMID": 965314}
{"id": "pubmed23n0032_6270", "title": "Mechanical apparatus for rigid suspension of the spinal column in mammals.", "content": "An apparatus is described for rigid suspension of the spinal columns of mammals ranging in size from large dogs to small rabbits. This apparatus is unique in that the animal is suspended on its side, allowing free access to the entire spinal column for spinal cord studies and free access to the thoracic and abdominal regions for heart, lung, and visceral studies that may require simultaneous surgical procedures in both ventral and dorsal regions.", "contents": "Mechanical apparatus for rigid suspension of the spinal column in mammals. An apparatus is described for rigid suspension of the spinal columns of mammals ranging in size from large dogs to small rabbits. This apparatus is unique in that the animal is suspended on its side, allowing free access to the entire spinal column for spinal cord studies and free access to the thoracic and abdominal regions for heart, lung, and visceral studies that may require simultaneous surgical procedures in both ventral and dorsal regions.", "PMID": 965315}
{"id": "pubmed23n0032_6271", "title": "Estimation of cardiac output by an N2O rebreathing method in adults and children.", "content": "A nitrous oxide rebreathing method was used to measure recirculation time and cardiac output (as pulmonary blood flow) in adults and children during exercise. The method was compared with the indirect (CO2) Fick rebreathing method in almost simultaneous measurements of cardiac output. Recirculation time varied from about 15 s at rest to 8.5 s at high levels of exercise, and was slightly longer in children than adults. Cardiac output estimated by both methods was within the normal range for children and adults, being lower for a given oxygen consumption in children. For the adults the mean intrasubject variability of the N2O rebreathing method, measured as the coefficient of variation of cardiac output, was in the range of 6-8% at all levels of work. This was lower than reported for other N2O rebreathing methods, and similar to values based on the indirect (CO2) Fick, direct (O2) Fick, and dye-dilution methods of estimating cardiac output. The method is therefore proposed as a noninvasive technique suitable for rapid, accurate, and reproducible measurements of cardiac output.", "contents": "Estimation of cardiac output by an N2O rebreathing method in adults and children. A nitrous oxide rebreathing method was used to measure recirculation time and cardiac output (as pulmonary blood flow) in adults and children during exercise. The method was compared with the indirect (CO2) Fick rebreathing method in almost simultaneous measurements of cardiac output. Recirculation time varied from about 15 s at rest to 8.5 s at high levels of exercise, and was slightly longer in children than adults. Cardiac output estimated by both methods was within the normal range for children and adults, being lower for a given oxygen consumption in children. For the adults the mean intrasubject variability of the N2O rebreathing method, measured as the coefficient of variation of cardiac output, was in the range of 6-8% at all levels of work. This was lower than reported for other N2O rebreathing methods, and similar to values based on the indirect (CO2) Fick, direct (O2) Fick, and dye-dilution methods of estimating cardiac output. The method is therefore proposed as a noninvasive technique suitable for rapid, accurate, and reproducible measurements of cardiac output.", "PMID": 965316}
{"id": "pubmed23n0032_6272", "title": "Simple method of hepatic venous blood sampling in the rat.", "content": "An improved method of hepatic venous blood sampling by catheterization in rats is proposed and applied to the measurement of hepatic blood flow (HBF) by the continuous ICG infusion method in normal rats and in rats treated chronically with CC1(4). The value of HBF determined by the present method was 0.90 +/- 0.17 (SD) ml/min per g liver under ether anesthesia, which was consistent with the reported values determined by other more complicated methods. The applicability of the present method was also shown in CC1(4) chronic intoxicated rats, to give 0.64 +/- 0.11 ml/min per g liver of HBF.", "contents": "Simple method of hepatic venous blood sampling in the rat. An improved method of hepatic venous blood sampling by catheterization in rats is proposed and applied to the measurement of hepatic blood flow (HBF) by the continuous ICG infusion method in normal rats and in rats treated chronically with CC1(4). The value of HBF determined by the present method was 0.90 +/- 0.17 (SD) ml/min per g liver under ether anesthesia, which was consistent with the reported values determined by other more complicated methods. The applicability of the present method was also shown in CC1(4) chronic intoxicated rats, to give 0.64 +/- 0.11 ml/min per g liver of HBF.", "PMID": 965317}
{"id": "pubmed23n0032_6273", "title": "Electrochemical sensor for continuous transcutaneous PCO2 measurement.", "content": "A sensor suitable for continuous transcutaneous PCO2 measurements is described. The sensor consists of an antimony-antimony oxide electrode in combination with a silver-silver chloride reference electrode, bathed in an electrolyte and covered by a Teflon membrane. A servo-controlled heater unit was used to maintain the sensor's temperature and to produce local hyperemia. The resulting oxidation-reduction potential under constant temperature is a linear function of the logarithm PCO2. Response time (95%) to step changes in PCO2 from 27 to 70 mmHg was 2.7 +/- 0.3 min. Following a 12-h \"aging\" time, the electrode exhibited a minimal drift of 5.2 +/- 2.2 mV for 16 h, representing an average PCO2 drift of 0.5 mmHg/h. This sensor was applied on three rabbits and on five human volunteers, and found satisfactory under normal physiological conditions.", "contents": "Electrochemical sensor for continuous transcutaneous PCO2 measurement. A sensor suitable for continuous transcutaneous PCO2 measurements is described. The sensor consists of an antimony-antimony oxide electrode in combination with a silver-silver chloride reference electrode, bathed in an electrolyte and covered by a Teflon membrane. A servo-controlled heater unit was used to maintain the sensor's temperature and to produce local hyperemia. The resulting oxidation-reduction potential under constant temperature is a linear function of the logarithm PCO2. Response time (95%) to step changes in PCO2 from 27 to 70 mmHg was 2.7 +/- 0.3 min. Following a 12-h \"aging\" time, the electrode exhibited a minimal drift of 5.2 +/- 2.2 mV for 16 h, representing an average PCO2 drift of 0.5 mmHg/h. This sensor was applied on three rabbits and on five human volunteers, and found satisfactory under normal physiological conditions.", "PMID": 965318}
{"id": "pubmed23n0032_6274", "title": "Collaborative study of accelerated development of latent fingerprint images on paper by application of steam.", "content": "Nine latent fingerprint examiners participated in a collaborative study in the use of a steam iron to accelerate chemical development of latent images on bond and newsprint papers treated with a 0.5% solution of ninhydrin in acetone. Four latent images were placed on each paper by the Associate Referee during an 8 hr period. The papers were paired and numbered 1, 2, and 3. Three pairs were mailed to each collaborator who treated Pair 1 by dipping, Pair 2 by brushing or swabbing, and Pair 3 by spraying. After air-drying, the papers were subjected to heat and steam from a household steam iron until the images developed. The average time for developing all images was 5.8 min/paper. Of the total images available, 92.1% were developed to some degree; 47.9% of these images or 43.5% of all images were identified by the collaborators. The method has been adopted as offical first action.", "contents": "Collaborative study of accelerated development of latent fingerprint images on paper by application of steam. Nine latent fingerprint examiners participated in a collaborative study in the use of a steam iron to accelerate chemical development of latent images on bond and newsprint papers treated with a 0.5% solution of ninhydrin in acetone. Four latent images were placed on each paper by the Associate Referee during an 8 hr period. The papers were paired and numbered 1, 2, and 3. Three pairs were mailed to each collaborator who treated Pair 1 by dipping, Pair 2 by brushing or swabbing, and Pair 3 by spraying. After air-drying, the papers were subjected to heat and steam from a household steam iron until the images developed. The average time for developing all images was 5.8 min/paper. Of the total images available, 92.1% were developed to some degree; 47.9% of these images or 43.5% of all images were identified by the collaborators. The method has been adopted as offical first action.", "PMID": 965322}
{"id": "pubmed23n0032_6275", "title": "Uses of field desorption mass spectrometry in the pharmaceutical industry.", "content": "Field desorption (FD) is a gentle method of ionizing molecules from the solid state. The method is still in its infancy and remains more of an art than a science. Purines, pyrimidines, pteridines, and nucleosides represent some of the compound classes which are studied repeatedly in our laboratory. Often the primary information sought is the molecular weight. Many of these compounds are thermally unstable or nonvolatile. In these instances, FD mass spectrometry has proven to be quite helpful. Molecular weights are obtained in a short time, eliminating the need for more time-consuming derivatization or chemical degradation methods. FD has been applied to the study of drug metabolism and has shown great promise in determining polar metabolites, including direct analysis of glucuronide, sulfate, and amino acid conjugates. Some problems have been encountered in handling samples of biological origins. Salt impurities introduced by the separation procedure may interfere with the desorption process. Cluster ions and background noise make it difficult to assign molecular ions, and some compounds do not yield molecular ions with FD.", "contents": "Uses of field desorption mass spectrometry in the pharmaceutical industry. Field desorption (FD) is a gentle method of ionizing molecules from the solid state. The method is still in its infancy and remains more of an art than a science. Purines, pyrimidines, pteridines, and nucleosides represent some of the compound classes which are studied repeatedly in our laboratory. Often the primary information sought is the molecular weight. Many of these compounds are thermally unstable or nonvolatile. In these instances, FD mass spectrometry has proven to be quite helpful. Molecular weights are obtained in a short time, eliminating the need for more time-consuming derivatization or chemical degradation methods. FD has been applied to the study of drug metabolism and has shown great promise in determining polar metabolites, including direct analysis of glucuronide, sulfate, and amino acid conjugates. Some problems have been encountered in handling samples of biological origins. Salt impurities introduced by the separation procedure may interfere with the desorption process. Cluster ions and background noise make it difficult to assign molecular ions, and some compounds do not yield molecular ions with FD.", "PMID": 965323}
{"id": "pubmed23n0032_6276", "title": "Applications of mass spectrometry to the analysis of mycotoxins.", "content": "Methods of identifying mycotoxins by mass spectrometry are reviewed, with special reference to the value of a complete listing of known fungal metabolites, arranged by molecular weight. Such a list is applied to the identification of various mycotoxins, and mass spectrometry is applied to the structural elucidation of dimethylnidurufin and ostreogrycin A.", "contents": "Applications of mass spectrometry to the analysis of mycotoxins. Methods of identifying mycotoxins by mass spectrometry are reviewed, with special reference to the value of a complete listing of known fungal metabolites, arranged by molecular weight. Such a list is applied to the identification of various mycotoxins, and mass spectrometry is applied to the structural elucidation of dimethylnidurufin and ostreogrycin A.", "PMID": 965324}
{"id": "pubmed23n0032_6277", "title": "Review of the metabolism and mode of action of cyclophosphamide.", "content": "A review of recent studies of the pharmacology of cyclophosphamide is presented. Particular attention is paid to the use of mass spectrometry in the identification of the active compound phosphoramide mustard as a circulating human metabolite of cyclophosphamide, the development of an assay method for cyclophosphamide, phosphoramide mustard, and nornitrogen mustard, and a demonstration that alkylation by phosphoramide mustard proceeds through a symmetrical aziridinium ion.", "contents": "Review of the metabolism and mode of action of cyclophosphamide. A review of recent studies of the pharmacology of cyclophosphamide is presented. Particular attention is paid to the use of mass spectrometry in the identification of the active compound phosphoramide mustard as a circulating human metabolite of cyclophosphamide, the development of an assay method for cyclophosphamide, phosphoramide mustard, and nornitrogen mustard, and a demonstration that alkylation by phosphoramide mustard proceeds through a symmetrical aziridinium ion.", "PMID": 965325}
{"id": "pubmed23n0032_6278", "title": "Gas-liquid chromatography and mass spectrometric ion selective detection of sterigmatocystin in grains.", "content": "A method was developed for direct gas-liquid chromatographic-mass spectrometric (GLC-MS) analysis of nonderivatized sterigmatocystin in wheat, rice, barley, and corn. Sterigmatocystin was extracted from spiked grain samples by 2 methods, both of which yielded high recoveries of 14C-labeled sterigmatocystin (greater than 90%). Extracts were cleaned up by gel permeation chromatography on polystyrene with tetrahydrofuran as the eluant. Chromatography of 14C-labeled compound indicated good recoveries (greater than 90%). The GLC-MS system was modified to give an all-glass chromatographic pathway to the ion source. Short glass or quartz columns packed with silicone liquid phase on inert supports gave the best results. Trace levels of sterigmatocystin were very labile in all gas chromatographic systems and levels below 5 ppb in grains were detected only with great difficulty. The GLC-MS system was utilized in the mass fragmentography mode, with selective ion monitoring of the m/e 295, 306, and 324 peaks. These ions were present in only trace levels in all control grain samples, indicating minimal interference from other compounds. The limit of accurate detectability (20 ng) on spiked grain samples was lower than previously reported. Lower levels of sterigmatocystin (1 ppb or 4 ng on column) could be detected in grains by using the mass fragmentography mode, but these levels could not be measured accurately.", "contents": "Gas-liquid chromatography and mass spectrometric ion selective detection of sterigmatocystin in grains. A method was developed for direct gas-liquid chromatographic-mass spectrometric (GLC-MS) analysis of nonderivatized sterigmatocystin in wheat, rice, barley, and corn. Sterigmatocystin was extracted from spiked grain samples by 2 methods, both of which yielded high recoveries of 14C-labeled sterigmatocystin (greater than 90%). Extracts were cleaned up by gel permeation chromatography on polystyrene with tetrahydrofuran as the eluant. Chromatography of 14C-labeled compound indicated good recoveries (greater than 90%). The GLC-MS system was modified to give an all-glass chromatographic pathway to the ion source. Short glass or quartz columns packed with silicone liquid phase on inert supports gave the best results. Trace levels of sterigmatocystin were very labile in all gas chromatographic systems and levels below 5 ppb in grains were detected only with great difficulty. The GLC-MS system was utilized in the mass fragmentography mode, with selective ion monitoring of the m/e 295, 306, and 324 peaks. These ions were present in only trace levels in all control grain samples, indicating minimal interference from other compounds. The limit of accurate detectability (20 ng) on spiked grain samples was lower than previously reported. Lower levels of sterigmatocystin (1 ppb or 4 ng on column) could be detected in grains by using the mass fragmentography mode, but these levels could not be measured accurately.", "PMID": 965326}
{"id": "pubmed23n0032_6279", "title": "High-performance liquid chromatographic determination of L-aspartyl-L-phenylalanine methyl ester in various food products and formulations.", "content": "A simple, rapid, and specific high-performance liquid chromatographic (HPLC) procedure is described for the analysis of the chemical sweetener L-aspartyl-L-phenylalanine methyl ester (aspartame). Using a strong cation exchange column and pressures less than 1000 psig, an analysis can be performed in less than 15 min. The technique has been applied to a wide range of food products and formulations. No interferences were found in the samples studied. Recoveries are quantitative, and the coefficients of variation for replicate analyses are less than or equal to 2.5%.", "contents": "High-performance liquid chromatographic determination of L-aspartyl-L-phenylalanine methyl ester in various food products and formulations. A simple, rapid, and specific high-performance liquid chromatographic (HPLC) procedure is described for the analysis of the chemical sweetener L-aspartyl-L-phenylalanine methyl ester (aspartame). Using a strong cation exchange column and pressures less than 1000 psig, an analysis can be performed in less than 15 min. The technique has been applied to a wide range of food products and formulations. No interferences were found in the samples studied. Recoveries are quantitative, and the coefficients of variation for replicate analyses are less than or equal to 2.5%.", "PMID": 965327}
{"id": "pubmed23n0032_6280", "title": "Impurities in commercial saccharin. I. Impurities soluble in organic solvents.", "content": "Thirteen saccharin samples used for carcinogenicity tests in animals in various laboratories were analyzed for their chemical purity. Although most of the impurities were water-soluble, some were mainly soluble in organic solvents. These impurities were extracted with chloroform-methanol from a water solution of sodium saccharin. Samples obtained as acid-saccharin were converted to the sodium form before extraction. The major impurity in commercial saccharin, o-toluenesulfonamide, was also soluble in this system. Impurities were separated by gas-liquid chromatography of the underivatized, concentrated extract. Eleven major, well separated peaks were collected from the gas chromatographic column and identified by mass spectroscopy. Some of the peaks were compared with known standards. Qualitative and quantitative differences in impurities were observed among different saccharin samples. The identified impurities (in order of appearance from the gas-liquid chromatographic column) were as follows: o-toluenesulfonamide; p-toluenesufonamide; 1,2-benzisothiazole 1,1-dioxide; 1,2-benzisothiazoline 1,1-dioxide; diphenylsulfone; 0,0'-ditolylsulfone; o,m'-ditolylsulfone, o,p'-ditolylsulfone; m,p'-ditolylsulfone; p,p'-ditolylsulfone, and tetracosane.", "contents": "Impurities in commercial saccharin. I. Impurities soluble in organic solvents. Thirteen saccharin samples used for carcinogenicity tests in animals in various laboratories were analyzed for their chemical purity. Although most of the impurities were water-soluble, some were mainly soluble in organic solvents. These impurities were extracted with chloroform-methanol from a water solution of sodium saccharin. Samples obtained as acid-saccharin were converted to the sodium form before extraction. The major impurity in commercial saccharin, o-toluenesulfonamide, was also soluble in this system. Impurities were separated by gas-liquid chromatography of the underivatized, concentrated extract. Eleven major, well separated peaks were collected from the gas chromatographic column and identified by mass spectroscopy. Some of the peaks were compared with known standards. Qualitative and quantitative differences in impurities were observed among different saccharin samples. The identified impurities (in order of appearance from the gas-liquid chromatographic column) were as follows: o-toluenesulfonamide; p-toluenesufonamide; 1,2-benzisothiazole 1,1-dioxide; 1,2-benzisothiazoline 1,1-dioxide; diphenylsulfone; 0,0'-ditolylsulfone; o,m'-ditolylsulfone, o,p'-ditolylsulfone; m,p'-ditolylsulfone; p,p'-ditolylsulfone, and tetracosane.", "PMID": 965328}
{"id": "pubmed23n0032_6281", "title": "Evaluation and confirmation of an alkylation-gas-liquid chromatographic method for the determination of carbamate and urea herbicides in foods.", "content": "An alkylation technique using methyl iodide and sodium hydride in dimethyl sulfoxide has been evaluated for 3 carbamate and 7 urea herbicides in 9 foods. Recoveries ranged from 56 to 113%, depending on herbicide, concentration, and food type. Reproducibility was about +/-6% at 0.1 and 1.0 ppm. Identities of the herbicides were confirmed at these and lower levels by cleaving the aniline moiety from the alkylated herbicides with sodium methoxide in methanol, followed by gas-liquid chromatography on the same column as the parent compound. An electrolytic conductivity detector in the nitrogen mode was used for all analyses. Minimum detectable levels were in the range of 0.005-0.01 ppm in the foods studied.", "contents": "Evaluation and confirmation of an alkylation-gas-liquid chromatographic method for the determination of carbamate and urea herbicides in foods. An alkylation technique using methyl iodide and sodium hydride in dimethyl sulfoxide has been evaluated for 3 carbamate and 7 urea herbicides in 9 foods. Recoveries ranged from 56 to 113%, depending on herbicide, concentration, and food type. Reproducibility was about +/-6% at 0.1 and 1.0 ppm. Identities of the herbicides were confirmed at these and lower levels by cleaving the aniline moiety from the alkylated herbicides with sodium methoxide in methanol, followed by gas-liquid chromatography on the same column as the parent compound. An electrolytic conductivity detector in the nitrogen mode was used for all analyses. Minimum detectable levels were in the range of 0.005-0.01 ppm in the foods studied.", "PMID": 965329}
{"id": "pubmed23n0032_6282", "title": "High-pressure liquid chromatographic analysis of urea herbicides in foods.", "content": "A method is described for the direct analysis of the urea herbicides linuron, monuron, diuron, chlorbromuron, fluometuron, chloroxuron, and fenuron in cabbage, corn, potatoes, turnip, and wheat at 0.01-1.0 ppm. The samples are extracted with acetone; the filtrate is partitioned with hexane-methylene chloride (1+1) followed by 2 other extractions with methylene chloride. The organic phases are dried and concentrated for column chromatographic cleanup on 2% deactivated Florisil. The column fractions are evaporated just to dryness and redissolved in 10 ml isooctane for high-pressure liquid chromatography on a 25 cm silica gel (5 mum) column. Isopropanol in isooctane is the mobile phase, and compounds are measured by ultraviolet absorption at 254 nm. Recoveries are greater than 80% in most cases. These results are confirmed by alkylation with sodium hydride-methyl iodide in dimethyl sulfoxide to form the methyl products which are analyzed by the same chromatographic system or by gas-liquid chromatography.", "contents": "High-pressure liquid chromatographic analysis of urea herbicides in foods. A method is described for the direct analysis of the urea herbicides linuron, monuron, diuron, chlorbromuron, fluometuron, chloroxuron, and fenuron in cabbage, corn, potatoes, turnip, and wheat at 0.01-1.0 ppm. The samples are extracted with acetone; the filtrate is partitioned with hexane-methylene chloride (1+1) followed by 2 other extractions with methylene chloride. The organic phases are dried and concentrated for column chromatographic cleanup on 2% deactivated Florisil. The column fractions are evaporated just to dryness and redissolved in 10 ml isooctane for high-pressure liquid chromatography on a 25 cm silica gel (5 mum) column. Isopropanol in isooctane is the mobile phase, and compounds are measured by ultraviolet absorption at 254 nm. Recoveries are greater than 80% in most cases. These results are confirmed by alkylation with sodium hydride-methyl iodide in dimethyl sulfoxide to form the methyl products which are analyzed by the same chromatographic system or by gas-liquid chromatography.", "PMID": 965330}
{"id": "pubmed23n0032_6283", "title": "Reference Raman spectra of ten phosphorus-containing pesticides.", "content": "The Raman spectra of malathion, ethion, methyl parathion, parathion, EPN, 0,0-diethyl-0-(2,4-dichlorophenyl) phosphorothioate, dichlorvos, mevinphos, tributyl phosphorotrithioite, and 2,4-dichlorobenzyltributylphosphonium chloride have been recorded. These spectra are presented, along with tables giving values for the frequencies.", "contents": "Reference Raman spectra of ten phosphorus-containing pesticides. The Raman spectra of malathion, ethion, methyl parathion, parathion, EPN, 0,0-diethyl-0-(2,4-dichlorophenyl) phosphorothioate, dichlorvos, mevinphos, tributyl phosphorotrithioite, and 2,4-dichlorobenzyltributylphosphonium chloride have been recorded. These spectra are presented, along with tables giving values for the frequencies.", "PMID": 965331}
{"id": "pubmed23n0032_6284", "title": "Gas-liquid chromatographic determination of chlorphoxim residues in water and fish by in-block methylation.", "content": "A gas-liquid chromatographic method has been developed for the analysis of residues of chlorphoxim, 2-chloro-alpha ((diethoxyphosphinothioyl)oxy)imino)-benzeneacetonitrile, in water and fish. The method is based on the in-block methylation of chlorphoxim with 0.01M trimethylanilinium hydroxide in methanol. The derivative, O,O-diethyl O-methyl phosphorothioate, was determined quantitatively by using a flame photometric detector specific for phosphorus. The in-block reaction is 70% efficient. Water samples were extracted with hexane; fish were extracted with methylene chloride and cleaned up on an acetonitrile-hexane partition column. Recoveries from water and fish samples spiked with chlorphoxim averaged 86.3 and 80.4%, respectively. Limits of detection were 10.0 ppb for 5 g samples of fish and 0.10 ppb for 300 ml water samples.", "contents": "Gas-liquid chromatographic determination of chlorphoxim residues in water and fish by in-block methylation. A gas-liquid chromatographic method has been developed for the analysis of residues of chlorphoxim, 2-chloro-alpha ((diethoxyphosphinothioyl)oxy)imino)-benzeneacetonitrile, in water and fish. The method is based on the in-block methylation of chlorphoxim with 0.01M trimethylanilinium hydroxide in methanol. The derivative, O,O-diethyl O-methyl phosphorothioate, was determined quantitatively by using a flame photometric detector specific for phosphorus. The in-block reaction is 70% efficient. Water samples were extracted with hexane; fish were extracted with methylene chloride and cleaned up on an acetonitrile-hexane partition column. Recoveries from water and fish samples spiked with chlorphoxim averaged 86.3 and 80.4%, respectively. Limits of detection were 10.0 ppb for 5 g samples of fish and 0.10 ppb for 300 ml water samples.", "PMID": 965332}
{"id": "pubmed23n0032_6285", "title": "Gas-liquid chromatographic determination of azinphos ethyl in human plasma and in mouse plasma, tissue, and fat.", "content": "A new method for the determination of azinphos ethyl (O,O-diethyl-S-(4-oxo-1,2,3-benzotriazin-3(4H)-ylmethyl) phosphorodithioate) in human plasma and in mouse plasma, tissue, and fat has been developed. The method is based on extraction with benzene or hexane and cleanup of fat and tissue samples by a minicolumn containing Florisil and sodium sulfate. Azinphos ethyl is eluted from the column with 10% acetonitrile in benzene and is concentrated to an appropriate volume for gas-liquid chromatographic analysis, using a 63Ni electron capture detector and a glass column containing 3% OV-1 on Gas-Chrom Q. The method is sensitive to 0.005 ppm in human plasma, 0.01 ppm in mouse plasma, 0.08 ppm in mouse liver, 0.05 ppm in mouse brain, and 0.10 ppm in mouse fat. The limit of detection is 2 pg; mean recoveries ranged from 96 to 98%.", "contents": "Gas-liquid chromatographic determination of azinphos ethyl in human plasma and in mouse plasma, tissue, and fat. A new method for the determination of azinphos ethyl (O,O-diethyl-S-(4-oxo-1,2,3-benzotriazin-3(4H)-ylmethyl) phosphorodithioate) in human plasma and in mouse plasma, tissue, and fat has been developed. The method is based on extraction with benzene or hexane and cleanup of fat and tissue samples by a minicolumn containing Florisil and sodium sulfate. Azinphos ethyl is eluted from the column with 10% acetonitrile in benzene and is concentrated to an appropriate volume for gas-liquid chromatographic analysis, using a 63Ni electron capture detector and a glass column containing 3% OV-1 on Gas-Chrom Q. The method is sensitive to 0.005 ppm in human plasma, 0.01 ppm in mouse plasma, 0.08 ppm in mouse liver, 0.05 ppm in mouse brain, and 0.10 ppm in mouse fat. The limit of detection is 2 pg; mean recoveries ranged from 96 to 98%.", "PMID": 965333}
{"id": "pubmed23n0032_6286", "title": "Ion selective electrode for 2,4-dichlorophenoxyacetic acid.", "content": "An electrode responsive to 2,4-D (2,4-dichlorophenoxyacetic acid) was constructed by dissolving tetrazolium derivatives as an ion exchanger in a liquid membrane. The electrode exhibited rapid and Nernstian response to solutions of 2,4-D over the concentration range 10(-1) to 10(-4)M. The presence of diverse substances such as acetate, benzoate, and 3-indoleacetate showed no appreciable effect on the electromotive force of the electrode.", "contents": "Ion selective electrode for 2,4-dichlorophenoxyacetic acid. An electrode responsive to 2,4-D (2,4-dichlorophenoxyacetic acid) was constructed by dissolving tetrazolium derivatives as an ion exchanger in a liquid membrane. The electrode exhibited rapid and Nernstian response to solutions of 2,4-D over the concentration range 10(-1) to 10(-4)M. The presence of diverse substances such as acetate, benzoate, and 3-indoleacetate showed no appreciable effect on the electromotive force of the electrode.", "PMID": 965334}
{"id": "pubmed23n0032_6287", "title": "Improved microbiological procedure for determining bacitracin in premixes and mixed feeds.", "content": "An improved microbiological procedure is presented for determining bacitracin in premixes and mixed feeds. Premixes are extracted with acidic 50% aqueous methanol, and mixed feeds are extracted with 50% dimethylformamide after washing with acetone to remove fats and pigments. Extracts are diluted with pH 6.0 phosphate buffer and assayed by using a 2-point assay system with Micrococcus flavus as the test organism. The simpler extraction coupled with the 2-point assay system measured bacitracin levels as low as 10 g/ton. Accuracy and repeatability of the 2-point system were at least equivalent to those for official AOAC methods.", "contents": "Improved microbiological procedure for determining bacitracin in premixes and mixed feeds. An improved microbiological procedure is presented for determining bacitracin in premixes and mixed feeds. Premixes are extracted with acidic 50% aqueous methanol, and mixed feeds are extracted with 50% dimethylformamide after washing with acetone to remove fats and pigments. Extracts are diluted with pH 6.0 phosphate buffer and assayed by using a 2-point assay system with Micrococcus flavus as the test organism. The simpler extraction coupled with the 2-point assay system measured bacitracin levels as low as 10 g/ton. Accuracy and repeatability of the 2-point system were at least equivalent to those for official AOAC methods.", "PMID": 965335}
{"id": "pubmed23n0032_6288", "title": "Gas-liquid chromatographic determination of chloramphenicol in agricultural crops.", "content": "A method is presented for the gas-liquid chromatographic determination of chloramphenicol in agricultural crops. Chloramphenicol is extracted with ethyl acetate, cleaned up by silicic acid column chromatography, acetylated with acetic anhydride and pyridine, and then measured by gas-liquid chromatography with electron capture detection. Two stationary phases, DEGS + phosphoric acid and Reoplex 400, were used. The sensitivity was ca 8 ng (40% full scale deflection). The efficicency of the analytical method was evaluated by analyzing crops fortified with chloramphenicol. The average recovery ranged from 72% in unpolished rice to 86% in Chinese radishes.", "contents": "Gas-liquid chromatographic determination of chloramphenicol in agricultural crops. A method is presented for the gas-liquid chromatographic determination of chloramphenicol in agricultural crops. Chloramphenicol is extracted with ethyl acetate, cleaned up by silicic acid column chromatography, acetylated with acetic anhydride and pyridine, and then measured by gas-liquid chromatography with electron capture detection. Two stationary phases, DEGS + phosphoric acid and Reoplex 400, were used. The sensitivity was ca 8 ng (40% full scale deflection). The efficicency of the analytical method was evaluated by analyzing crops fortified with chloramphenicol. The average recovery ranged from 72% in unpolished rice to 86% in Chinese radishes.", "PMID": 965336}
{"id": "pubmed23n0032_6289", "title": "Colorimetric reagents for determining vitamin A in feeds and foods.", "content": "Selected blue-color developing reagents for determining vitamin A, effects of some carotenoids, tocopherol, and sterols commonly found in vitamin A extracts of feeds and foods, and effects of moisture and light on the colors developed under normal laboratory conditions were studied. Similar blue colors were obtained using SbCl3, CF3COOH, and CCl3COOH in solutions of CHCl3, CH2Cl2 and C2H4Cl2. In general, the reagents in CH2Cl2 solutions produced the most stable blue colors. The CCl3COOH-in-CH2Cl2 reagent developed slightly higher initial color, but CCl3COOH, especially in CHCl3 solution, was the least stable reagent. Small amounts of moisture affected only SbCl3 reagents, by forming cloudy solutions and precipitates. Carotene and monohydroxy and dihydroxy carotenoids reacted with all reagents tested to produce bluish colors, which, however, reached maximum development much later than the vitamin A blue colors. The presence of sterols slightly decreased color production of vitamin A solutions and frequently slightly increased rate of fading, especially for SbCl3 reagents. With proper precautions any of the reagents studied may be used to determine vitamin A. The preferred reagent for general use probably is CF3COOH-in-CH2Cl2, but it is not the most economical.", "contents": "Colorimetric reagents for determining vitamin A in feeds and foods. Selected blue-color developing reagents for determining vitamin A, effects of some carotenoids, tocopherol, and sterols commonly found in vitamin A extracts of feeds and foods, and effects of moisture and light on the colors developed under normal laboratory conditions were studied. Similar blue colors were obtained using SbCl3, CF3COOH, and CCl3COOH in solutions of CHCl3, CH2Cl2 and C2H4Cl2. In general, the reagents in CH2Cl2 solutions produced the most stable blue colors. The CCl3COOH-in-CH2Cl2 reagent developed slightly higher initial color, but CCl3COOH, especially in CHCl3 solution, was the least stable reagent. Small amounts of moisture affected only SbCl3 reagents, by forming cloudy solutions and precipitates. Carotene and monohydroxy and dihydroxy carotenoids reacted with all reagents tested to produce bluish colors, which, however, reached maximum development much later than the vitamin A blue colors. The presence of sterols slightly decreased color production of vitamin A solutions and frequently slightly increased rate of fading, especially for SbCl3 reagents. With proper precautions any of the reagents studied may be used to determine vitamin A. The preferred reagent for general use probably is CF3COOH-in-CH2Cl2, but it is not the most economical.", "PMID": 965337}
{"id": "pubmed23n0032_6290", "title": "Collaborative study of the determination of sodium in dietetic foods by the sodium ion electrode method.", "content": "The dietetic food sample is diluted with total ionic strength adjusted buffer. The electrode potential of the sample is measured, known increments of sodium are added, and the electrode potential is measured after each addition. The mv readings obtained are plotted on Gran's paper and the concentration of sodium in the sample is found from the intercept of the extrapolated straight line. Seven processed dietetic vegetables and 2 spiked samples were sent to 7 collaborators. There was a 95.8% agreement between the results from the 7 collaborators and the Associate Referee for the unfortified samples. Recoveries of 98.5 and 95.1% were obtained on fortified samples, based on results from 5 collaborators. The statistical analysis indicates minimal bias and adequate precision for determination of sodium in dietetic food at the current regulatory requirements. The method has been adopted as official first action.", "contents": "Collaborative study of the determination of sodium in dietetic foods by the sodium ion electrode method. The dietetic food sample is diluted with total ionic strength adjusted buffer. The electrode potential of the sample is measured, known increments of sodium are added, and the electrode potential is measured after each addition. The mv readings obtained are plotted on Gran's paper and the concentration of sodium in the sample is found from the intercept of the extrapolated straight line. Seven processed dietetic vegetables and 2 spiked samples were sent to 7 collaborators. There was a 95.8% agreement between the results from the 7 collaborators and the Associate Referee for the unfortified samples. Recoveries of 98.5 and 95.1% were obtained on fortified samples, based on results from 5 collaborators. The statistical analysis indicates minimal bias and adequate precision for determination of sodium in dietetic food at the current regulatory requirements. The method has been adopted as official first action.", "PMID": 965338}
{"id": "pubmed23n0032_6291", "title": "Collaborative study of methods for the determination of chloride, citrate, and phosphate in processed cheese.", "content": "A collaborative study was conducted to compare the International Dairy Federation (IDF)/International Organization for Standardization (ISO)/Association of Official Analytical Chemists (AOAC) proposed methods for the determination of chloride and citrate in processed cheese with the corresponding current AOAC methods. Since there is no current AOAC method for phosphate, only the IDF/ISO/AOAC method was used. Five samples of processed cheese were prepared with varying added amounts of the anions of interest. The samples were analyzed in duplicate by 7 collaborating laboratories. For chloride, both methods are comparable, but since the IDF/ISO/AOAC method is simpler and faster it has been adopted as official first action to replace the current AOAC method, 16.224. For citrate, the colorimetric IDF/ISO/AOAC method is more rapid and simpler than the current AOAC gravimetric method. However, the AOAC method is more precise. Therefore, the proposed method has been adopted as official first action as an alternative to 16.237. For phosphate, 2 of the 7 laboratories were outliers by the Youden ranking test but the results by the remaining laboratories appear satisfactory. The phosphate method should receive further study to reduce the between-laboratory error component.", "contents": "Collaborative study of methods for the determination of chloride, citrate, and phosphate in processed cheese. A collaborative study was conducted to compare the International Dairy Federation (IDF)/International Organization for Standardization (ISO)/Association of Official Analytical Chemists (AOAC) proposed methods for the determination of chloride and citrate in processed cheese with the corresponding current AOAC methods. Since there is no current AOAC method for phosphate, only the IDF/ISO/AOAC method was used. Five samples of processed cheese were prepared with varying added amounts of the anions of interest. The samples were analyzed in duplicate by 7 collaborating laboratories. For chloride, both methods are comparable, but since the IDF/ISO/AOAC method is simpler and faster it has been adopted as official first action to replace the current AOAC method, 16.224. For citrate, the colorimetric IDF/ISO/AOAC method is more rapid and simpler than the current AOAC gravimetric method. However, the AOAC method is more precise. Therefore, the proposed method has been adopted as official first action as an alternative to 16.237. For phosphate, 2 of the 7 laboratories were outliers by the Youden ranking test but the results by the remaining laboratories appear satisfactory. The phosphate method should receive further study to reduce the between-laboratory error component.", "PMID": 965339}
{"id": "pubmed23n0032_6292", "title": "Spectrophotometric determination of ferric, ferrous, and total iron in drugs by reaction with alpha, alpha'-dipyridyl.", "content": "The drug sample is dissolved in 0.2N HCl to yield a final concentration of 30 mug Fe/ml, an aliquot is reacted with alpha,alpha-dipyridyl, and the pH is adjusted with an acetate buffer. For Fe(II), the color intensity is measured at 523 nm. For total iron, ascorbic acid is added to a separate aliquot of the sample solution, reducing all the iron present to Fe(II), which is then reacted with alpha,alpha'-dipyridyl, diluted to volume, and read at 523 nm. The Fe(III) content is determined by subtracing Fe(II) from total iron. This method is applicable to iron-containing drugs and drug dosage forms.", "contents": "Spectrophotometric determination of ferric, ferrous, and total iron in drugs by reaction with alpha, alpha'-dipyridyl. The drug sample is dissolved in 0.2N HCl to yield a final concentration of 30 mug Fe/ml, an aliquot is reacted with alpha,alpha-dipyridyl, and the pH is adjusted with an acetate buffer. For Fe(II), the color intensity is measured at 523 nm. For total iron, ascorbic acid is added to a separate aliquot of the sample solution, reducing all the iron present to Fe(II), which is then reacted with alpha,alpha'-dipyridyl, diluted to volume, and read at 523 nm. The Fe(III) content is determined by subtracing Fe(II) from total iron. This method is applicable to iron-containing drugs and drug dosage forms.", "PMID": 965340}
{"id": "pubmed23n0032_6293", "title": "Investigation and identification of the bromination products of dimethoxyamphetamines.", "content": "The qualitative analysis of the aromatic bromination products of the 6 isomeric dimethoxyamphetamines and their hydrochloride or hydrobromide salts is described. Their ultraviolet, mass, and proton magnetic resonance spectra are not sufficiently different for distinction but infrared spectra allow a positive identification to be made and reference spectra are provided for the bromination products of 2,4-, 2,5-, 2,6-, 4,5-, and 3,5-dimethoxyamphetamines. The application of gas-liquid and thin layer chromatography for the analysis of these products is discussed. The bromination of 2,3-dimethoxyamphetamine consistently gave mixtures which could not be separated satisfactorily; spectra are included for completeness of the comparison of products.", "contents": "Investigation and identification of the bromination products of dimethoxyamphetamines. The qualitative analysis of the aromatic bromination products of the 6 isomeric dimethoxyamphetamines and their hydrochloride or hydrobromide salts is described. Their ultraviolet, mass, and proton magnetic resonance spectra are not sufficiently different for distinction but infrared spectra allow a positive identification to be made and reference spectra are provided for the bromination products of 2,4-, 2,5-, 2,6-, 4,5-, and 3,5-dimethoxyamphetamines. The application of gas-liquid and thin layer chromatography for the analysis of these products is discussed. The bromination of 2,3-dimethoxyamphetamine consistently gave mixtures which could not be separated satisfactorily; spectra are included for completeness of the comparison of products.", "PMID": 965341}
{"id": "pubmed23n0032_6294", "title": "Extraction and partition chromatography of tolbutamide as ion-pairs with quaternary ammonium cations.", "content": "The partition of tolbutamide (1-butyl-3-(p-tolylsulfonyl) urea) as ion-pairs with homologous tetraalkyl ammonium cations was studied. The determination of experimental extraction constants permitted quantitative calculation of distribution ratios, in agreement with theoretical relationships, over a continuous range of about one billion. The nature and the concentration of the counter-ion and solvent composition were the variables studied. Based on the theoretical results, a rapid partition chromatographic procedure was devised. A solution of tolbutamide sample in 10% aqueous tetraethyl ammonium hydroxide is incorporated in the system as the immobile phase in the partition column. A 1+1 mixture of chloroform and isooctane removes extraneous materials; then chloroform elutes the tolbutamide-tetraethyl ammonium ion-pair, which is converted to the free acid by passing the eluate through a phosphoric acid segment, and tolbutamide is determined spectrophotometrically without further treatment. Standard recoveries averaged 100.5+/-0.70%; commercial tablets assayed 100.5+/-0.85%.", "contents": "Extraction and partition chromatography of tolbutamide as ion-pairs with quaternary ammonium cations. The partition of tolbutamide (1-butyl-3-(p-tolylsulfonyl) urea) as ion-pairs with homologous tetraalkyl ammonium cations was studied. The determination of experimental extraction constants permitted quantitative calculation of distribution ratios, in agreement with theoretical relationships, over a continuous range of about one billion. The nature and the concentration of the counter-ion and solvent composition were the variables studied. Based on the theoretical results, a rapid partition chromatographic procedure was devised. A solution of tolbutamide sample in 10% aqueous tetraethyl ammonium hydroxide is incorporated in the system as the immobile phase in the partition column. A 1+1 mixture of chloroform and isooctane removes extraneous materials; then chloroform elutes the tolbutamide-tetraethyl ammonium ion-pair, which is converted to the free acid by passing the eluate through a phosphoric acid segment, and tolbutamide is determined spectrophotometrically without further treatment. Standard recoveries averaged 100.5+/-0.70%; commercial tablets assayed 100.5+/-0.85%.", "PMID": 965342}
{"id": "pubmed23n0032_6295", "title": "Rapid gas-liquid chromatographic method for determining residues of ethephon (2-chloroethyl phosphonic acid) in tomatoes.", "content": "A rapid method is described for the determination of the growth regulator ethephon (2-chloroethyl phosphonic acid) in tomatoes. Samples are extracted with ethyl acetate. The extract is methylated, decolorized with carbon, and analyzed by gas-liquid chromatography on a column packed with 15% QF-1/10% DC-200 on Chromosorb W (HP). A flame photometric detector is used. Recoveries from tomatoes fortified with 0.1-11.0 ppm ethephon were 78-98%. The sensitivity of this method is about 0.1 ppm.", "contents": "Rapid gas-liquid chromatographic method for determining residues of ethephon (2-chloroethyl phosphonic acid) in tomatoes. A rapid method is described for the determination of the growth regulator ethephon (2-chloroethyl phosphonic acid) in tomatoes. Samples are extracted with ethyl acetate. The extract is methylated, decolorized with carbon, and analyzed by gas-liquid chromatography on a column packed with 15% QF-1/10% DC-200 on Chromosorb W (HP). A flame photometric detector is used. Recoveries from tomatoes fortified with 0.1-11.0 ppm ethephon were 78-98%. The sensitivity of this method is about 0.1 ppm.", "PMID": 965343}
{"id": "pubmed23n0032_6296", "title": "Gas-liquid chromatographic determination of levamisole residues in bovine milk.", "content": "A gas-liquid chromatographic (GLC) method is described for determining residues of levamisole, (1-)-(2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole), in milk. Levamisole is extracted from an alkaline milk sample with water-saturated ethyl acetate. After co-extractives are removed by various partitionings, the levamisole concentration is measured by GLC by using an alkali (RbSO4) flame ionization detector, vs. an external standard. This method is satisfactory for determining levamisole residues as low as 0.01 ppm in bovine milk. Apparent residues in control milk samples averaged less than 0.001 ppm. Recoveries of 0.01-2.0 ppm added levamisole ranged from 86.5 to 113%. Animals treated with various Tramisol formulations showed apparent levamisole residues below the validated sensitivity of the method (0.01 ppm) at sampling periods exceeding 48 hr.", "contents": "Gas-liquid chromatographic determination of levamisole residues in bovine milk. A gas-liquid chromatographic (GLC) method is described for determining residues of levamisole, (1-)-(2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole), in milk. Levamisole is extracted from an alkaline milk sample with water-saturated ethyl acetate. After co-extractives are removed by various partitionings, the levamisole concentration is measured by GLC by using an alkali (RbSO4) flame ionization detector, vs. an external standard. This method is satisfactory for determining levamisole residues as low as 0.01 ppm in bovine milk. Apparent residues in control milk samples averaged less than 0.001 ppm. Recoveries of 0.01-2.0 ppm added levamisole ranged from 86.5 to 113%. Animals treated with various Tramisol formulations showed apparent levamisole residues below the validated sensitivity of the method (0.01 ppm) at sampling periods exceeding 48 hr.", "PMID": 965344}
{"id": "pubmed23n0032_6297", "title": "Bis-diazotized benzidine as a spray reagent for detecting zearalenone on thin layer chromatoplates.", "content": "Bis-diazotized benzidine is a highly sensitive spray reagent for detecting zearalenone (F-2) in corn samples. As little as 2.0 ng of this Fusarium toxin could be visualized on a thin layer plate after development. In addition, this spray reagent is more specific than the spray reagents currently used for zearalenone.", "contents": "Bis-diazotized benzidine as a spray reagent for detecting zearalenone on thin layer chromatoplates. Bis-diazotized benzidine is a highly sensitive spray reagent for detecting zearalenone (F-2) in corn samples. As little as 2.0 ng of this Fusarium toxin could be visualized on a thin layer plate after development. In addition, this spray reagent is more specific than the spray reagents currently used for zearalenone.", "PMID": 965345}
{"id": "pubmed23n0032_6298", "title": "Determination of sterigmatocystin in corn and oats by gel permeation and high-pressure liquid chromatography.", "content": "Corn and oats samples are extracted with acetonitrile-water, followed by partition of the extract against hexane, transfer to chloroform, and elution from a silica gel column. The extract is purified by gel permeation chromatography on an automatic instrument. Reverse phase high-pressure liquid chromatography, using a 254 nm ultraviolet detector and 0.1 M KH2PO4-acetonitrile (7+5) as the mobile phase, is used for quantitation. The average recovery from 6 samples of corn to which 0, 25, 50, and 100 mug sterigmatocystin/kg had been added was 59%, with a coefficient of variation of 8.4%. The average recovery from oats fortified at the same levels was 74%, with a coefficient of variation of 12%. A confirmation procedure based on hemiacetal derivative formation on a thin layer chromatographic plate is also described.", "contents": "Determination of sterigmatocystin in corn and oats by gel permeation and high-pressure liquid chromatography. Corn and oats samples are extracted with acetonitrile-water, followed by partition of the extract against hexane, transfer to chloroform, and elution from a silica gel column. The extract is purified by gel permeation chromatography on an automatic instrument. Reverse phase high-pressure liquid chromatography, using a 254 nm ultraviolet detector and 0.1 M KH2PO4-acetonitrile (7+5) as the mobile phase, is used for quantitation. The average recovery from 6 samples of corn to which 0, 25, 50, and 100 mug sterigmatocystin/kg had been added was 59%, with a coefficient of variation of 8.4%. The average recovery from oats fortified at the same levels was 74%, with a coefficient of variation of 12%. A confirmation procedure based on hemiacetal derivative formation on a thin layer chromatographic plate is also described.", "PMID": 965346}
{"id": "pubmed23n0032_6299", "title": "Ion selective method for the determination of nitrite in smoked fish.", "content": "In a direct ion selective electrode method for determining nitrite in smoked fish, the nitrite extracted from the sample is converted to nitrous acid with a measured addition of an acid buffer. The released nitrous acid is measured by a nitrogen oxide electrode. A known addition procedure is used to accurately measure the amount of nitrite in solution and the nitrite concentration is determined directly from Gran's plot paper. Recoveries of nitrite from spiked smoked chub samples ranged from 94.0 to 100.0% with an average of 96.7 and a relative standard deviation of +/-1.9% at the 100 ppm level. Results from the ion selective method were comparable with those from the official first action AOAC method 24.037-24.038.", "contents": "Ion selective method for the determination of nitrite in smoked fish. In a direct ion selective electrode method for determining nitrite in smoked fish, the nitrite extracted from the sample is converted to nitrous acid with a measured addition of an acid buffer. The released nitrous acid is measured by a nitrogen oxide electrode. A known addition procedure is used to accurately measure the amount of nitrite in solution and the nitrite concentration is determined directly from Gran's plot paper. Recoveries of nitrite from spiked smoked chub samples ranged from 94.0 to 100.0% with an average of 96.7 and a relative standard deviation of +/-1.9% at the 100 ppm level. Results from the ion selective method were comparable with those from the official first action AOAC method 24.037-24.038.", "PMID": 965347}
{"id": "pubmed23n0032_6300", "title": "Oxidative phosphorylation in brown adipose tissue mitochondria from rats kept under normal environmental conditions.", "content": "Based on criteria such as the ADP/O ratio and respiratory control by ADP, the energy-coupling efficiency of brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions for a long time decreased remarkably. The presence of bovine serum albumin, GTP, or ATP plus carnitine in the reaction medium markedly increased the efficiency of oxidative phosphorylation of brown adipose tissue mitochondria. Pre-treatment of brown adipose tissue mitochondria with 2% bovine serum albumin, GTP, or ATP plus carnitine caused a decrease in the amount of free fatty acids bound to the mitochondria from 13.1 to 7.0, 9.0, or 8.2 mug per mg protein, respectively. Removal of the free fatty acids by means of these pre-treatments resulted in restoration of efficient oxidative phosphorylation; there was a correlation between the amount of free fatty acids removed and the degree of recovery in the respiratory control ratio. The elimination of only a fraction of the free fatty acids, as little as 4 mug per mg protein, was sufficient to ensure respiratory control by ADP. It appears that the free fatty acids which lie mainly outside the inner mitochondrial membrane are responsible for the decrease in the efficiency of oxidative phosphorylation in brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions.", "contents": "Oxidative phosphorylation in brown adipose tissue mitochondria from rats kept under normal environmental conditions. Based on criteria such as the ADP/O ratio and respiratory control by ADP, the energy-coupling efficiency of brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions for a long time decreased remarkably. The presence of bovine serum albumin, GTP, or ATP plus carnitine in the reaction medium markedly increased the efficiency of oxidative phosphorylation of brown adipose tissue mitochondria. Pre-treatment of brown adipose tissue mitochondria with 2% bovine serum albumin, GTP, or ATP plus carnitine caused a decrease in the amount of free fatty acids bound to the mitochondria from 13.1 to 7.0, 9.0, or 8.2 mug per mg protein, respectively. Removal of the free fatty acids by means of these pre-treatments resulted in restoration of efficient oxidative phosphorylation; there was a correlation between the amount of free fatty acids removed and the degree of recovery in the respiratory control ratio. The elimination of only a fraction of the free fatty acids, as little as 4 mug per mg protein, was sufficient to ensure respiratory control by ADP. It appears that the free fatty acids which lie mainly outside the inner mitochondrial membrane are responsible for the decrease in the efficiency of oxidative phosphorylation in brown adipose tissue mitochondria isolated from rats kept under normal environmental conditions.", "PMID": 965363}
{"id": "pubmed23n0032_6301", "title": "Enzyme immunoassay of testosterone using the testosterone-glucoamylase complex.", "content": "A highly sensitive, reproducible method was established for enzyme-coupled immunoassay of testosterone, involving coupling of testosterone to glucoamylase [EC 3.2.1.3] with a water-soluble coupling reagen carbodimide. No enzyme activity was lost during this coupling procedure. The sensitivity of the method was comparable to that of competitive protein binding assay.", "contents": "Enzyme immunoassay of testosterone using the testosterone-glucoamylase complex. A highly sensitive, reproducible method was established for enzyme-coupled immunoassay of testosterone, involving coupling of testosterone to glucoamylase [EC 3.2.1.3] with a water-soluble coupling reagen carbodimide. No enzyme activity was lost during this coupling procedure. The sensitivity of the method was comparable to that of competitive protein binding assay.", "PMID": 965364}
{"id": "pubmed23n0032_6302", "title": "Further studies on the specificity of the minor ribonuclease from Aspergillus saitoi.", "content": "In order to investigate the base specificity of the minor RNase [EC 3.1.4.23] from Aspergillus saitoi, the kinetic constant of the enzyme was measured with 16 dinucleoside phosphates (XpY's) as substrates at pH 5.5 and 25 degrees. The maximum rates of transesterification of GpY's were in the range of 10,000 to 2,800 and were markedly larger than those of other XpY's, including XpG's. The average Km values of UpY, CpY, ApY, and GpY increased in the order A, C, U, and G. This order coincides with that of the rates of release of 4 common nucleotides from RNA by RNase Ms (the rates decreased in the order 3'-GMP, 3'-AMP, 3'-CMP, and 3'-UMP), except for the case of GpY. Therefore the rates of release of nucleotides seem to be dependent on the affinity constant of the X base in XpY, except in the case of GpY. The high rate of release of guanylic acid from RNA was explained by the findings that higher rates of hydrolysis of GpY's compensate for their lower affinity to the enzyme. These results suggested that the base specificity was rather dependent upon the X nucleotide in XpY. The Ki values of various nucleotides and nucleosides towards RNase Ms were measured. These compounds inhibited the RNase competitively. Although the inhibitory effect depends on the bases, sugars and location of phosphate, when the location of phosphate on the sugar was the same, the Ki values of ribonucleotides decreased in the order U, G, C, and A and those of deoxyribonucleotides decreased in the order T, G, C, and A. The dependence of the inhibitory effect of ribonucleosides on the bases was similar to that of ribonucleotides, but that of deoxyribonucleosides was in the order dT, dA, dG, and dC.", "contents": "Further studies on the specificity of the minor ribonuclease from Aspergillus saitoi. In order to investigate the base specificity of the minor RNase [EC 3.1.4.23] from Aspergillus saitoi, the kinetic constant of the enzyme was measured with 16 dinucleoside phosphates (XpY's) as substrates at pH 5.5 and 25 degrees. The maximum rates of transesterification of GpY's were in the range of 10,000 to 2,800 and were markedly larger than those of other XpY's, including XpG's. The average Km values of UpY, CpY, ApY, and GpY increased in the order A, C, U, and G. This order coincides with that of the rates of release of 4 common nucleotides from RNA by RNase Ms (the rates decreased in the order 3'-GMP, 3'-AMP, 3'-CMP, and 3'-UMP), except for the case of GpY. Therefore the rates of release of nucleotides seem to be dependent on the affinity constant of the X base in XpY, except in the case of GpY. The high rate of release of guanylic acid from RNA was explained by the findings that higher rates of hydrolysis of GpY's compensate for their lower affinity to the enzyme. These results suggested that the base specificity was rather dependent upon the X nucleotide in XpY. The Ki values of various nucleotides and nucleosides towards RNase Ms were measured. These compounds inhibited the RNase competitively. Although the inhibitory effect depends on the bases, sugars and location of phosphate, when the location of phosphate on the sugar was the same, the Ki values of ribonucleotides decreased in the order U, G, C, and A and those of deoxyribonucleotides decreased in the order T, G, C, and A. The dependence of the inhibitory effect of ribonucleosides on the bases was similar to that of ribonucleotides, but that of deoxyribonucleosides was in the order dT, dA, dG, and dC.", "PMID": 965365}
{"id": "pubmed23n0032_6303", "title": "Identification of glucosyl sphingosine from Gaucher's spleen by gas chromatography-electron impact and GC-chemical ionization mass spectrometry.", "content": "Glucosyl sphingosine was isolated from the spleen of a patient with adult-type Gaucher's disease. The yield of purified glucosyl sphingosine was 9.3 nmoles/g wet tissue. The gas chromatography-chemical ionization mass spectrum of acetylated glucosyl sphingosine showed peaks due to the presence of ions formed by successive loss of acetic acid (mass 60) from the molecular ion (QM+, 714). Fragments from acetylated sugar, m/e 331, and from the sphingosine residue, m/e 306, were also detected. The GC-CI mass spectrum of the trimethylsilyl derivative of glucosyl sphingosine showed peaks due to the molecular ion (QM+, 822), ions from the sugar moiety (m/e 361, 271), and ions from the sphingosine base (m/e 264, 280). Fragmentation analysis of the purified sample by GC-EI and GC-CI mass spectrometry confirmed the structure glucopyranosyl(1 leads to 1)-1,3-dihydroxy-2-amino-4-octadecene.", "contents": "Identification of glucosyl sphingosine from Gaucher's spleen by gas chromatography-electron impact and GC-chemical ionization mass spectrometry. Glucosyl sphingosine was isolated from the spleen of a patient with adult-type Gaucher's disease. The yield of purified glucosyl sphingosine was 9.3 nmoles/g wet tissue. The gas chromatography-chemical ionization mass spectrum of acetylated glucosyl sphingosine showed peaks due to the presence of ions formed by successive loss of acetic acid (mass 60) from the molecular ion (QM+, 714). Fragments from acetylated sugar, m/e 331, and from the sphingosine residue, m/e 306, were also detected. The GC-CI mass spectrum of the trimethylsilyl derivative of glucosyl sphingosine showed peaks due to the molecular ion (QM+, 822), ions from the sugar moiety (m/e 361, 271), and ions from the sphingosine base (m/e 264, 280). Fragmentation analysis of the purified sample by GC-EI and GC-CI mass spectrometry confirmed the structure glucopyranosyl(1 leads to 1)-1,3-dihydroxy-2-amino-4-octadecene.", "PMID": 965366}
{"id": "pubmed23n0032_6304", "title": "Additional evidence for hydrophobic bond formation in the adsorption of sulfanilamide on bio-gel beads.", "content": "We have previously suggested the involvement of both hydrogen binding and hydrophobic bonding in the adsorption of sulfanilamide on Bio-Gel beads. In the present study, we closely examined the concentration dependence of the binding curve and our proposed binding model has been corroborated. For comparison, binding parameters and thermodynamic data pertaining to the sulfanilamide-Sephadex system have been also evaluated.", "contents": "Additional evidence for hydrophobic bond formation in the adsorption of sulfanilamide on bio-gel beads. We have previously suggested the involvement of both hydrogen binding and hydrophobic bonding in the adsorption of sulfanilamide on Bio-Gel beads. In the present study, we closely examined the concentration dependence of the binding curve and our proposed binding model has been corroborated. For comparison, binding parameters and thermodynamic data pertaining to the sulfanilamide-Sephadex system have been also evaluated.", "PMID": 965367}
{"id": "pubmed23n0032_6305", "title": "The clinic waiting room. Environment for health education via television.", "content": "Parents and children in a clinic waiting room are motivated toward better health and constitute a receptive audience for health education via the familiar medium of television. A videotape cassette covering materials from four areas of child health care was displayed in an out-patient waiting area, using a recorder-player and monitor. Parents were interviewed prior to the introduction of the video program, immediately after the program, and again by telephone two weeks after exposure to the program. Analysis of the interviews revealed increases in knowledge about child health, as well as positive attitudes toward the clinic. The familiarity, availability, and nonthreatening nature of the medium were deemed advantages of this format.", "contents": "The clinic waiting room. Environment for health education via television. Parents and children in a clinic waiting room are motivated toward better health and constitute a receptive audience for health education via the familiar medium of television. A videotape cassette covering materials from four areas of child health care was displayed in an out-patient waiting area, using a recorder-player and monitor. Parents were interviewed prior to the introduction of the video program, immediately after the program, and again by telephone two weeks after exposure to the program. Analysis of the interviews revealed increases in knowledge about child health, as well as positive attitudes toward the clinic. The familiarity, availability, and nonthreatening nature of the medium were deemed advantages of this format.", "PMID": 965370}
{"id": "pubmed23n0032_6306", "title": "Biochemical characterization of collagens synthesized by fibroblasts derived from normal and diseased human gingiva.", "content": "Fibroblasts obtained from healthy and diseased human gingiva were labeled with radioactive amino acids and the collagenous proteins synthesized were studied. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of untreated, reduced, and pepsin-treated proteins of the medium and cell extract showed that the collagenous proteins synthesized by these cells exist in the precursor form. Type I collagen was the chief constituent. In addition, cells from normal tissue synthesized type III collagen in amounts varying from 5 to 30%. Type III collagen was not detected in the cultures of fibroblasts from diseased tissue; however, an additional collagen fractionated between 2.5 to 5.0 M NaCl and accounted for 22 to 29% of the total. This collagen had an alpha1/alpha2 ratio of 8.6 and hydroxylysine/lysine ratio and cyanogen bromide peptide pattern were similar to that of alpha1[I]. It is concluded that the fibroblasts derived from disease gingiva synthesize a collagen of composition (alpha1)3, probably of type I.", "contents": "Biochemical characterization of collagens synthesized by fibroblasts derived from normal and diseased human gingiva. Fibroblasts obtained from healthy and diseased human gingiva were labeled with radioactive amino acids and the collagenous proteins synthesized were studied. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of untreated, reduced, and pepsin-treated proteins of the medium and cell extract showed that the collagenous proteins synthesized by these cells exist in the precursor form. Type I collagen was the chief constituent. In addition, cells from normal tissue synthesized type III collagen in amounts varying from 5 to 30%. Type III collagen was not detected in the cultures of fibroblasts from diseased tissue; however, an additional collagen fractionated between 2.5 to 5.0 M NaCl and accounted for 22 to 29% of the total. This collagen had an alpha1/alpha2 ratio of 8.6 and hydroxylysine/lysine ratio and cyanogen bromide peptide pattern were similar to that of alpha1[I]. It is concluded that the fibroblasts derived from disease gingiva synthesize a collagen of composition (alpha1)3, probably of type I.", "PMID": 965371}
{"id": "pubmed23n0032_6307", "title": "Studies on the attachment of carbohydrate to ovalbumin nascent chains in hen oviduct.", "content": "We have examined the question, is the carbohydrate moiety of ovalbumin attached to nascent chains or to the completed protein after its release from polyribosomes. Ovalbumin nascent chains have been isolated from hen oviduct by immunoprecipitation of ovalbumin-synthesizing polysomes and subsequent isolation of peptidyl-tRNA by DEAE-cellulose chromatography. Our results indicate that both glucosamine and mannose are incorporated into ovalbumin nascent chains by hen oviduct fragments. Although the asparagine to which the carbohydrates are attached is 10 amino acid residues from the NH2 terminus, the majority of the carbohydrate, if not all, is attached only to those peptide chains that have been essentially completed.", "contents": "Studies on the attachment of carbohydrate to ovalbumin nascent chains in hen oviduct. We have examined the question, is the carbohydrate moiety of ovalbumin attached to nascent chains or to the completed protein after its release from polyribosomes. Ovalbumin nascent chains have been isolated from hen oviduct by immunoprecipitation of ovalbumin-synthesizing polysomes and subsequent isolation of peptidyl-tRNA by DEAE-cellulose chromatography. Our results indicate that both glucosamine and mannose are incorporated into ovalbumin nascent chains by hen oviduct fragments. Although the asparagine to which the carbohydrates are attached is 10 amino acid residues from the NH2 terminus, the majority of the carbohydrate, if not all, is attached only to those peptide chains that have been essentially completed.", "PMID": 965372}
{"id": "pubmed23n0032_6308", "title": "Purification and properties of sterol carrier protein1.", "content": "Previous studies have demonstrated that both the 105,000 X g soluble supernatant (S105) and microsomal membranes from rat liver are required for the enzymatic conversion of squalene to cholesterol (Scallen, T.J., Dean, W.J., and Schuster, M.W. (1968) J. Biol. Chem. 243, 5202). It was postulated that S105 contained a noncatalytic carrier protein which was required for this enzymatic process (Scallen, T. J., Schuster, M.W., and Dhar, A.K. (1971) J. Biol. Chem. 246, 224). Later evidence demonstrated that S105 contained at least two proteins which were required for the microsomal conversion of squalene to cholesterol (Scallen, T.J., Srikantaiah, M.V., Seetharam, B., Hansbury, E., and Gavey, K.L. (1974) Fed. Proc. 33, 1733). This article describes the purification and properties of the first of these soluble proteins, sterol carrier protein1 (SCP1), which has been purified 575-fold from rat liver S105. While SCP1 specifically activated the enzymatic conversion of squalene to lanosterol by liver microsomal membranes, SCP1 possessed no capacity to activate the microsomal conversion of [3H-A14,4-dimethyl-delta8-cholestenol to C27 sterols or of [3H]7-dehydrocholesterol to cholesterol. Lanosterol was identified by silicic acid chromatography and mass spectrometry. The formation of lanosterol was a hyperbolic function of the concentration of SCP1 present in the incubation mixture. The Km observed for SCP1 was similar to the Km observed for squalene. The formation of lanosterol from squalene required FAD. The addition of phosphatidylserine increased enzymatic activity; however, phosphatidylserine was not required for this conversion. SCP1 was catalytically inactive when it was incubated with [3H] squalene and cofactors in the absence of microsomes. Substantial evidence supports the hypothesis that SCP1 operates as a noncatalytic carrier protein for the water-insoluble substrate squalene in the enzymatic conversion of squalene to lanosterol by liver microsomal membranes.", "contents": "Purification and properties of sterol carrier protein1. Previous studies have demonstrated that both the 105,000 X g soluble supernatant (S105) and microsomal membranes from rat liver are required for the enzymatic conversion of squalene to cholesterol (Scallen, T.J., Dean, W.J., and Schuster, M.W. (1968) J. Biol. Chem. 243, 5202). It was postulated that S105 contained a noncatalytic carrier protein which was required for this enzymatic process (Scallen, T. J., Schuster, M.W., and Dhar, A.K. (1971) J. Biol. Chem. 246, 224). Later evidence demonstrated that S105 contained at least two proteins which were required for the microsomal conversion of squalene to cholesterol (Scallen, T.J., Srikantaiah, M.V., Seetharam, B., Hansbury, E., and Gavey, K.L. (1974) Fed. Proc. 33, 1733). This article describes the purification and properties of the first of these soluble proteins, sterol carrier protein1 (SCP1), which has been purified 575-fold from rat liver S105. While SCP1 specifically activated the enzymatic conversion of squalene to lanosterol by liver microsomal membranes, SCP1 possessed no capacity to activate the microsomal conversion of [3H-A14,4-dimethyl-delta8-cholestenol to C27 sterols or of [3H]7-dehydrocholesterol to cholesterol. Lanosterol was identified by silicic acid chromatography and mass spectrometry. The formation of lanosterol was a hyperbolic function of the concentration of SCP1 present in the incubation mixture. The Km observed for SCP1 was similar to the Km observed for squalene. The formation of lanosterol from squalene required FAD. The addition of phosphatidylserine increased enzymatic activity; however, phosphatidylserine was not required for this conversion. SCP1 was catalytically inactive when it was incubated with [3H] squalene and cofactors in the absence of microsomes. Substantial evidence supports the hypothesis that SCP1 operates as a noncatalytic carrier protein for the water-insoluble substrate squalene in the enzymatic conversion of squalene to lanosterol by liver microsomal membranes.", "PMID": 965373}
{"id": "pubmed23n0032_6309", "title": "Shortening of the poly(A) region of mouse globin messenger RNA.", "content": "Nucleated erythroid cells isolated from the spleens of anemic mice were used to investigate the processing of the polyadenylic acid region of globin mRNA. Cells were labeled in media containing [3H] adenosine and transferred to media containing no radioactive precursor and incubated further in the presence or absence of actinomycin D. After various times following the transfer of the cells, globin mRNA was isolated using a combination of oligo(dT)-cellulose affinity chromatography, sucrose density centrifugation, and globin cDNA (the complementary DNA copy of globin mRNA)-cellulose affinity chromatography. The size of the poly(A) region was determined by polyacrylamide gel electrophoresis of the T1 and pancreatic RNase-resistant fragments. The prelabeled poly(A) region which initially comprises approximately 150 adenylate residues was found to become shorter with time, both in cells incubated in medium containing no radioactive precursor and in the presence of actinomycin D. After 9 h of incubation in the presence of actinomycin D, two major size classes of poly(A) were observed, one containing 35 to 45 adenylic acid residues and the other containing 55 to 65 residues. These two size classes are similar to those found in circulating reticulocytes suggesting that the poly(A) shortening observed in these cell incubation studies is similar to that which occurs in vivo. Two protein synthesis inhibitors, emetine and cycloheximide, were investigated with respect to their effect on poly(A) shortening. Neither drug inhibited the shortening of the poly(A) region of globin mRNA, suggesting that protein synthesis is not required for this process to occur.", "contents": "Shortening of the poly(A) region of mouse globin messenger RNA. Nucleated erythroid cells isolated from the spleens of anemic mice were used to investigate the processing of the polyadenylic acid region of globin mRNA. Cells were labeled in media containing [3H] adenosine and transferred to media containing no radioactive precursor and incubated further in the presence or absence of actinomycin D. After various times following the transfer of the cells, globin mRNA was isolated using a combination of oligo(dT)-cellulose affinity chromatography, sucrose density centrifugation, and globin cDNA (the complementary DNA copy of globin mRNA)-cellulose affinity chromatography. The size of the poly(A) region was determined by polyacrylamide gel electrophoresis of the T1 and pancreatic RNase-resistant fragments. The prelabeled poly(A) region which initially comprises approximately 150 adenylate residues was found to become shorter with time, both in cells incubated in medium containing no radioactive precursor and in the presence of actinomycin D. After 9 h of incubation in the presence of actinomycin D, two major size classes of poly(A) were observed, one containing 35 to 45 adenylic acid residues and the other containing 55 to 65 residues. These two size classes are similar to those found in circulating reticulocytes suggesting that the poly(A) shortening observed in these cell incubation studies is similar to that which occurs in vivo. Two protein synthesis inhibitors, emetine and cycloheximide, were investigated with respect to their effect on poly(A) shortening. Neither drug inhibited the shortening of the poly(A) region of globin mRNA, suggesting that protein synthesis is not required for this process to occur.", "PMID": 965374}
{"id": "pubmed23n0032_6310", "title": "Purification of a toxic protein from scorpion venom which activates the action potential Na+ ionophore.", "content": "Venom of the scorpion Leiurus quinquestriatus acts cooperatively with the alkaloids veratridine, aconitine, and batrachotoxin in activating the action potential Na+ ionophore. A small (Mr = 6700), basic (pI approximately 9.8), toxic polypeptide purified approximately 80-fold from this venom by ion exchange chromatography appears homogeneous by gel electrophoresis and isoelectric focusing and, like whole venom, acts cooperatively with the alkaloids veratridine, aconitine, and batrachotoxin to activate the action potential Na+ ionophore. The action of the scorpion toxin is slowly reversible. Concentration-response curves suggest interaction with a single class of sites with KD - 1.3 to 2.4 nM. The scorpion toxin is a poor activator of the Na+ ionophore when tested alone. However, treatment of cells sequentially with scorpion toxin followed by veratridine activates as well as treatment with both simultaneously suggesting that scorpion toxin binds in the absence of veratridine but does not activate the Na+ ionophore unless veratridine is present. In contrast, scorpion toxin causes 3- to 20-fold decreases in apparent KD for aconitine, veratridine, and batrachotoxin. The effect of the toxin is inhibited competitively by divalent cations and noncompetitively by tetrodotoxin (KI - 4 nM).", "contents": "Purification of a toxic protein from scorpion venom which activates the action potential Na+ ionophore. Venom of the scorpion Leiurus quinquestriatus acts cooperatively with the alkaloids veratridine, aconitine, and batrachotoxin in activating the action potential Na+ ionophore. A small (Mr = 6700), basic (pI approximately 9.8), toxic polypeptide purified approximately 80-fold from this venom by ion exchange chromatography appears homogeneous by gel electrophoresis and isoelectric focusing and, like whole venom, acts cooperatively with the alkaloids veratridine, aconitine, and batrachotoxin to activate the action potential Na+ ionophore. The action of the scorpion toxin is slowly reversible. Concentration-response curves suggest interaction with a single class of sites with KD - 1.3 to 2.4 nM. The scorpion toxin is a poor activator of the Na+ ionophore when tested alone. However, treatment of cells sequentially with scorpion toxin followed by veratridine activates as well as treatment with both simultaneously suggesting that scorpion toxin binds in the absence of veratridine but does not activate the Na+ ionophore unless veratridine is present. In contrast, scorpion toxin causes 3- to 20-fold decreases in apparent KD for aconitine, veratridine, and batrachotoxin. The effect of the toxin is inhibited competitively by divalent cations and noncompetitively by tetrodotoxin (KI - 4 nM).", "PMID": 965375}
{"id": "pubmed23n0032_6311", "title": "Biochemical evidence that Semliki Forest virus obtains its envelope from the plasma membrane of the host cell.", "content": "The data from chemical studies and electron microscopy suggest that Semliki Forest virus obtains its envelope by budding into the medium from the plasma membrane of the host cell. Biochemical evidence for this phenomenon, however, has not been published. Therefore, we undertook a series of pulse-chase studies so that we might quantitatively evaluate the importance of the budding mechanism in the morphogenesis of Semliki Forest virus. Baby hamster kidney cells (clone 13) were grown in culture and infected with Semliki Forest virus. The cells were exposed to [4,5-3H] leucine for 20 min and the subsequent incorporation of the label into virus proteins associated with cytoplasmic membranes and extracellular virus was determined. Initial experiments were conducted with microsomes and a precursor-product relationship was demonstrated between viral proteins in the microsomes and in extracellular virus. Further studies were performed with endoplasmic reticulum and plasma membrane preparations. Maximal incorporation of [3H] leucine was observed in the viral proteins located in the endoplasmic reticulum at the end of a 20-min pulse period; greater than 50% of this activity had disappeared within 2 h. The plasma membrane fraction contained no radioactivity at the end of the pulse period; subsequently, maximal labeling of the viral proteins in the plasma membrane occurred 4 h into the chase period and these labeled proteins had disappeared from this membrane 11 h after the pulse. At this time maximal incorporation of the labeled proteins into extracellular virus was observed. These data are consistent with a precursor-product relationship between the viral proteins in the endoplasmic reticulum which migrate to the plasma membrane and are subsequently incorporated into extracellular virus. All the radioactivity in the extracellular virus appears to have been derived from viral proteins associated with the plasma membrane of the cell. Therefore, mechanisms for the morphogenesis of Semliki Forest virus (in baby hamster kidney cells), other than budding from the plasma membrane, are unlikely to be of quantitative importance.", "contents": "Biochemical evidence that Semliki Forest virus obtains its envelope from the plasma membrane of the host cell. The data from chemical studies and electron microscopy suggest that Semliki Forest virus obtains its envelope by budding into the medium from the plasma membrane of the host cell. Biochemical evidence for this phenomenon, however, has not been published. Therefore, we undertook a series of pulse-chase studies so that we might quantitatively evaluate the importance of the budding mechanism in the morphogenesis of Semliki Forest virus. Baby hamster kidney cells (clone 13) were grown in culture and infected with Semliki Forest virus. The cells were exposed to [4,5-3H] leucine for 20 min and the subsequent incorporation of the label into virus proteins associated with cytoplasmic membranes and extracellular virus was determined. Initial experiments were conducted with microsomes and a precursor-product relationship was demonstrated between viral proteins in the microsomes and in extracellular virus. Further studies were performed with endoplasmic reticulum and plasma membrane preparations. Maximal incorporation of [3H] leucine was observed in the viral proteins located in the endoplasmic reticulum at the end of a 20-min pulse period; greater than 50% of this activity had disappeared within 2 h. The plasma membrane fraction contained no radioactivity at the end of the pulse period; subsequently, maximal labeling of the viral proteins in the plasma membrane occurred 4 h into the chase period and these labeled proteins had disappeared from this membrane 11 h after the pulse. At this time maximal incorporation of the labeled proteins into extracellular virus was observed. These data are consistent with a precursor-product relationship between the viral proteins in the endoplasmic reticulum which migrate to the plasma membrane and are subsequently incorporated into extracellular virus. All the radioactivity in the extracellular virus appears to have been derived from viral proteins associated with the plasma membrane of the cell. Therefore, mechanisms for the morphogenesis of Semliki Forest virus (in baby hamster kidney cells), other than budding from the plasma membrane, are unlikely to be of quantitative importance.", "PMID": 965376}
{"id": "pubmed23n0032_6312", "title": "Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta.", "content": "The active protein components of initiation factor M2B (IF-M2B) have been resolved into two homogeneous factors. These proteins, IF-M2Balpha and IF-M2Bbeta, were purified 300- and 500-fold, respectively, with a yield of about 15% of the original starting activity. The low molecular weight (approximately 17,000) of these two proteins is in contrast with the much greater molecular weights that have been found for other initiation factors. IF-M2Balpha is also unique among the initiation factors in that it contains no tryptophan and is capable of self-association. Both proteins are required for model assays which utilize 40 S and 60 S subunits (poly(U)-directed polyphenylalanine synthesis or AUG-directed methionyl-puromycin synthesis). IF-M2Bbeta has been shown to be required for hemoglobin synthesis, however, the presence of high concentrations of IF-M2Balpha in the 100,000 X g lysate supernatant has precluded the demonstration of an IF-M2Balpha requirement in hemoglobin synthesis.", "contents": "Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta. The active protein components of initiation factor M2B (IF-M2B) have been resolved into two homogeneous factors. These proteins, IF-M2Balpha and IF-M2Bbeta, were purified 300- and 500-fold, respectively, with a yield of about 15% of the original starting activity. The low molecular weight (approximately 17,000) of these two proteins is in contrast with the much greater molecular weights that have been found for other initiation factors. IF-M2Balpha is also unique among the initiation factors in that it contains no tryptophan and is capable of self-association. Both proteins are required for model assays which utilize 40 S and 60 S subunits (poly(U)-directed polyphenylalanine synthesis or AUG-directed methionyl-puromycin synthesis). IF-M2Bbeta has been shown to be required for hemoglobin synthesis, however, the presence of high concentrations of IF-M2Balpha in the 100,000 X g lysate supernatant has precluded the demonstration of an IF-M2Balpha requirement in hemoglobin synthesis.", "PMID": 965377}
{"id": "pubmed23n0032_6313", "title": "Effect of single and double peptide bond scission by trypsin on the structure and activity of staphylococcal enterotoxin C.", "content": "Two peptide bonds of staphylococcal enterotoxin C, were hydrolyzed concurrently at quite different rates during limited digestion with trypsin. A Lys-Val at about position 92 in the disulfide loop was the first bond cleaved, followed by a Lys-Asx at about position 57 on the NH2-terminal side of the loop. Preparations of singly cleaved material (enterotoxin C1-T1) contained about 93% of the cleaved protein and 7% unreacted enterotoxin. Preparations of the doubly cleaved material (enterotoxin C1-T2) consisted of 98% enterotoxin C1-T2 and 2% enterotoxin C1-T1. In the absence of denaturant, enterotoxin C1-T2 behaved as a single particle. It gave a single peak on Sephadex G-75 with a sedimentation coefficient of 2.85 S and a molecular weight of 29,100 by sedimentation equilibrium. Circular dichroic spectra indicated only minor conformational differences between enterotoxins C1-T2 and C1. However conformational stability was significantly affected with the unfolding of enterotoxin C1-T2 in 4 M guanidine hydrochloride proceeding at about twice the rate of native enterotoxin. Enterotoxin C1-T2 was separated into 6,500 and 22,000 molecular weight polypeptides by gel filtration on Sepharose 6B in 6 M guanidine hydrochloride. Complementation (as measured by CD spectra, serologic activity and mitogenicity) of the two polypeptides was readily achieved from solution in 6 M guanidine hydrochloride by dialysis against phosphate buffer. The 22,000 molecular weight polypeptide was further separated into two peptides (Mr = 4,000 and 19,000 after alkylation of the reduced disulfide bridge. Summation of the amino acid composition of the constituent peptides of enterotoxin C1-T2 agreed well with the composition of enterotoxin C1. A comparison of the 6,500 and 4,000 molecular weight polypeptides from enterotoxin C1-T2 with structurally equivalent segments of enterotoxin B suggested structural homology between the two antigenic variants. Enterotoxins C1, C1-T1, and C1-T2 gave reactions of complete identity in Ouchterlony immunodiffusion and were indistinguishable in the quantitative precipitin reaction. Enterotoxins C1-T1 and C1-T2 were highly mitogenic but were slightly less potent than the native enterotoxin. Enterotoxin C1-T2 had equivalent emetic activity to enterotoxin C1 in rhesus monkeys. It is suggested that the exceptional lability to limited enzymic hydrolysis exemplified by enterotoxin C1 is associated with beta turn structures at protein surfaces.", "contents": "Effect of single and double peptide bond scission by trypsin on the structure and activity of staphylococcal enterotoxin C. Two peptide bonds of staphylococcal enterotoxin C, were hydrolyzed concurrently at quite different rates during limited digestion with trypsin. A Lys-Val at about position 92 in the disulfide loop was the first bond cleaved, followed by a Lys-Asx at about position 57 on the NH2-terminal side of the loop. Preparations of singly cleaved material (enterotoxin C1-T1) contained about 93% of the cleaved protein and 7% unreacted enterotoxin. Preparations of the doubly cleaved material (enterotoxin C1-T2) consisted of 98% enterotoxin C1-T2 and 2% enterotoxin C1-T1. In the absence of denaturant, enterotoxin C1-T2 behaved as a single particle. It gave a single peak on Sephadex G-75 with a sedimentation coefficient of 2.85 S and a molecular weight of 29,100 by sedimentation equilibrium. Circular dichroic spectra indicated only minor conformational differences between enterotoxins C1-T2 and C1. However conformational stability was significantly affected with the unfolding of enterotoxin C1-T2 in 4 M guanidine hydrochloride proceeding at about twice the rate of native enterotoxin. Enterotoxin C1-T2 was separated into 6,500 and 22,000 molecular weight polypeptides by gel filtration on Sepharose 6B in 6 M guanidine hydrochloride. Complementation (as measured by CD spectra, serologic activity and mitogenicity) of the two polypeptides was readily achieved from solution in 6 M guanidine hydrochloride by dialysis against phosphate buffer. The 22,000 molecular weight polypeptide was further separated into two peptides (Mr = 4,000 and 19,000 after alkylation of the reduced disulfide bridge. Summation of the amino acid composition of the constituent peptides of enterotoxin C1-T2 agreed well with the composition of enterotoxin C1. A comparison of the 6,500 and 4,000 molecular weight polypeptides from enterotoxin C1-T2 with structurally equivalent segments of enterotoxin B suggested structural homology between the two antigenic variants. Enterotoxins C1, C1-T1, and C1-T2 gave reactions of complete identity in Ouchterlony immunodiffusion and were indistinguishable in the quantitative precipitin reaction. Enterotoxins C1-T1 and C1-T2 were highly mitogenic but were slightly less potent than the native enterotoxin. Enterotoxin C1-T2 had equivalent emetic activity to enterotoxin C1 in rhesus monkeys. It is suggested that the exceptional lability to limited enzymic hydrolysis exemplified by enterotoxin C1 is associated with beta turn structures at protein surfaces.", "PMID": 965378}
{"id": "pubmed23n0032_6314", "title": "Purification, size, and properties of the complex of orotate phosphoribosyltransferase: orotidylate decarboxylase from mouse Ehrlich ascites carcinoma.", "content": "Complex U, which contains the last two enzymes (orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidylate decarboxylase (EC 4.1.23)) of the six enzymes for the de novo biosynthesis of UMP, has been purified 200-fold from mouse Ehrlich ascites cells. The specific activity of the orotate phosphoribosyltransferase and the orotidylate decarboxylase activities of the complex were 0.115 and 0.290 mumol of product/mg of protein/min; the recovery of the activities was high being 20 to 30%. The rate of the two activities remained similar to that of the homogenate. At the sixth step of the fractionation, one can obtain a fraction that has lost phosphoribosyltransferase activity but retains decarboxylase activity. The apparent molecular weights, as determined by density gradient centrifugation, of the native complex and the fraction containing only decarboxylase activity are identical, 55,700 +/- 4,000. Both activities of complex U are labile to very mild treatments such as dilution, dialysis, or storage at 3 degrees. Dithiothreitol and 5-phosphoribosyl-1-pyrophosphate (PP-Rib-P), but not orotic acid or MgCl2, can stabilize either or both of the enzyme activities. The degree of stabilization by three of these chemicals varies with the reagent(s) used, with the nature of the treatment, and with the concentration of Complex U. When PP-Rib-P, Mg2+ and dithiothreitol are present in the diluting buffer the activity losses were slowed and then followed by a partial recovery of the phosphoribosyltransferase activity. Maximum activities of both enzymes are observed by adding undiluted complex to a complete reaction mixture without preincubation. The complex cannot be exposed to pH values of 4 or below, or pH 9 or above. The stability studies have led to the development of conditions that permit one, for the first time, to subject the complex to electrophoresis and to recover a large percentage of both enzyme activities, rather than only decarboxylase activity as has occurred in the past. The electrophoretic studies indicate that PP-Rib-P produces a complex whose conformation and/or net charge differ significantly from that of the complex in the absence of PP-Rib-P. Kinetic characteristics of the transferase are a pH optimum between 6.5 and 7.5, apparent Km values for orotate, PP-Rib-P, and Mg2+ of 1.9 muM, 16 muM, and 2.9 mM, respectively; for the decarboxylase, a sharp pH optimum of 7.0 is observed, and a Km value for orotidine 5'-phosphate of 0.8 muM.", "contents": "Purification, size, and properties of the complex of orotate phosphoribosyltransferase: orotidylate decarboxylase from mouse Ehrlich ascites carcinoma. Complex U, which contains the last two enzymes (orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidylate decarboxylase (EC 4.1.23)) of the six enzymes for the de novo biosynthesis of UMP, has been purified 200-fold from mouse Ehrlich ascites cells. The specific activity of the orotate phosphoribosyltransferase and the orotidylate decarboxylase activities of the complex were 0.115 and 0.290 mumol of product/mg of protein/min; the recovery of the activities was high being 20 to 30%. The rate of the two activities remained similar to that of the homogenate. At the sixth step of the fractionation, one can obtain a fraction that has lost phosphoribosyltransferase activity but retains decarboxylase activity. The apparent molecular weights, as determined by density gradient centrifugation, of the native complex and the fraction containing only decarboxylase activity are identical, 55,700 +/- 4,000. Both activities of complex U are labile to very mild treatments such as dilution, dialysis, or storage at 3 degrees. Dithiothreitol and 5-phosphoribosyl-1-pyrophosphate (PP-Rib-P), but not orotic acid or MgCl2, can stabilize either or both of the enzyme activities. The degree of stabilization by three of these chemicals varies with the reagent(s) used, with the nature of the treatment, and with the concentration of Complex U. When PP-Rib-P, Mg2+ and dithiothreitol are present in the diluting buffer the activity losses were slowed and then followed by a partial recovery of the phosphoribosyltransferase activity. Maximum activities of both enzymes are observed by adding undiluted complex to a complete reaction mixture without preincubation. The complex cannot be exposed to pH values of 4 or below, or pH 9 or above. The stability studies have led to the development of conditions that permit one, for the first time, to subject the complex to electrophoresis and to recover a large percentage of both enzyme activities, rather than only decarboxylase activity as has occurred in the past. The electrophoretic studies indicate that PP-Rib-P produces a complex whose conformation and/or net charge differ significantly from that of the complex in the absence of PP-Rib-P. Kinetic characteristics of the transferase are a pH optimum between 6.5 and 7.5, apparent Km values for orotate, PP-Rib-P, and Mg2+ of 1.9 muM, 16 muM, and 2.9 mM, respectively; for the decarboxylase, a sharp pH optimum of 7.0 is observed, and a Km value for orotidine 5'-phosphate of 0.8 muM.", "PMID": 965379}
{"id": "pubmed23n0032_6315", "title": "Hysteretic response of human erythrocyte pyruvate kinase to phosphoenolpyruvate. Potential role in regulation of 2,3-bisphosphoglycerate metabolism.", "content": "Human erythrocyte pyruvate kinase (EC 2.7.1.40, ATP-pyruvate phosphotransferase) was found to display a time-dependent activation (lag phase) in the reaction progress curves. The extent of this lag phase depended upon the treatment of the enzyme prior to assay. Preincubation of the enzyme with adenine nucleotides amplified the lag, whereas prior treatment with phosphoenolpyruvate diminished it. The activation process was first order in enzyme with the pseudo first order rate constants being a hyperbolic function of phosphoenolpyruvate concentration. The data provide evidence for a phosphoenol-pyruvate-mediated conversion of the enzyme to a more active form. Studies with the irreversible sulfhydryl inhibitor, N-ethylmaleimide (MalNEt), provided additional evidence for different conformational states of the enzyme induced by its substrates and effectors. Adenine nucleotides were found to promote inactivation by MalNEt and phosphoenolpyruvate protected against MalNEt. The possible metabolic significance of this \"hysteretic\" pyruvate kinase is discussed in relation to the known role of this enzyme in 2,3-bisphosphoglycerate metabolism (Rose, I.A. (1971) Exp. Eye Res. 11, 264-272).", "contents": "Hysteretic response of human erythrocyte pyruvate kinase to phosphoenolpyruvate. Potential role in regulation of 2,3-bisphosphoglycerate metabolism. Human erythrocyte pyruvate kinase (EC 2.7.1.40, ATP-pyruvate phosphotransferase) was found to display a time-dependent activation (lag phase) in the reaction progress curves. The extent of this lag phase depended upon the treatment of the enzyme prior to assay. Preincubation of the enzyme with adenine nucleotides amplified the lag, whereas prior treatment with phosphoenolpyruvate diminished it. The activation process was first order in enzyme with the pseudo first order rate constants being a hyperbolic function of phosphoenolpyruvate concentration. The data provide evidence for a phosphoenol-pyruvate-mediated conversion of the enzyme to a more active form. Studies with the irreversible sulfhydryl inhibitor, N-ethylmaleimide (MalNEt), provided additional evidence for different conformational states of the enzyme induced by its substrates and effectors. Adenine nucleotides were found to promote inactivation by MalNEt and phosphoenolpyruvate protected against MalNEt. The possible metabolic significance of this \"hysteretic\" pyruvate kinase is discussed in relation to the known role of this enzyme in 2,3-bisphosphoglycerate metabolism (Rose, I.A. (1971) Exp. Eye Res. 11, 264-272).", "PMID": 965380}
{"id": "pubmed23n0032_6316", "title": "Role of gamma-carboxyglutamic acid. An unusual protein transition required for the calcium-dependent binding of prothrombin to phospholipid.", "content": "A first order calcium-dependent transition can be monitored by a decrease in the intrinsic fluorescence of the isolated \"pro\" (Fragment1) region of prothrombin. The maximum fluorescence change is -40% for Fragment 1, and only about -6% for whole prothrombin. The most remarkable features of this transition are its rate and activation energy. The half-life for the transition at 0 degrees is about 100 min, and the temperature dependence shows an activation energy of 21 kcal/mol. The rate constant for the forward reaction is zero order in calcium and is not affected by the presence of phospholipid membranes. The equilibrium for the transition, however, is affected by phospholipid. At 30 degrees, [Ca]eq (the calcium concentration where half of the protein has undergone the transition) is 0.4 mM and the Hill coefficient is 2.6. Under the same conditions but in the presence of phospholipid [Ca]eq is 0.24 mM and the Hill coefficient is about 4.5. The transition is triggered by binding 3 or 4 calcium ions. The rate of Fragment 1 binding to phospholipid vesicles was tested using gel filtration techniques at 0 degrees. The rate constants, activation energy, and [Ca]eq values for this process were shown to correspond to the properties of the fluorescence change. The rate constants, activation energy, and Hill coefficients for binding of whole prothrombin to phospholipid correspond to the same parameters for Fragment 1 but the [Ca]eq values are lower. At 0 degrees, the [Ca]eq is 0.19 mM for the prothrombin transition and 0.1 mM for the transition in the presence of phospholipid. These results demonstrate that Fragment 1 and prothrombin undergo a transition when exposed to calcium ions which necessarily precedes protein-phospholipid interactions. In addition to its role in determining the correct protein structure, calcium plays a second role in prothrombin-phos-pholipid interaction which is in the actual formation of the protein-phospholipid bond. The [Ca]eq for binding protein (after its transition) to phospholipid is about 0.06 mM.", "contents": "Role of gamma-carboxyglutamic acid. An unusual protein transition required for the calcium-dependent binding of prothrombin to phospholipid. A first order calcium-dependent transition can be monitored by a decrease in the intrinsic fluorescence of the isolated \"pro\" (Fragment1) region of prothrombin. The maximum fluorescence change is -40% for Fragment 1, and only about -6% for whole prothrombin. The most remarkable features of this transition are its rate and activation energy. The half-life for the transition at 0 degrees is about 100 min, and the temperature dependence shows an activation energy of 21 kcal/mol. The rate constant for the forward reaction is zero order in calcium and is not affected by the presence of phospholipid membranes. The equilibrium for the transition, however, is affected by phospholipid. At 30 degrees, [Ca]eq (the calcium concentration where half of the protein has undergone the transition) is 0.4 mM and the Hill coefficient is 2.6. Under the same conditions but in the presence of phospholipid [Ca]eq is 0.24 mM and the Hill coefficient is about 4.5. The transition is triggered by binding 3 or 4 calcium ions. The rate of Fragment 1 binding to phospholipid vesicles was tested using gel filtration techniques at 0 degrees. The rate constants, activation energy, and [Ca]eq values for this process were shown to correspond to the properties of the fluorescence change. The rate constants, activation energy, and Hill coefficients for binding of whole prothrombin to phospholipid correspond to the same parameters for Fragment 1 but the [Ca]eq values are lower. At 0 degrees, the [Ca]eq is 0.19 mM for the prothrombin transition and 0.1 mM for the transition in the presence of phospholipid. These results demonstrate that Fragment 1 and prothrombin undergo a transition when exposed to calcium ions which necessarily precedes protein-phospholipid interactions. In addition to its role in determining the correct protein structure, calcium plays a second role in prothrombin-phos-pholipid interaction which is in the actual formation of the protein-phospholipid bond. The [Ca]eq for binding protein (after its transition) to phospholipid is about 0.06 mM.", "PMID": 965381}
{"id": "pubmed23n0032_6317", "title": "Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate.", "content": "Translation of HeLa cell RNA containing poly(A) in a wheat germ cell-free system is markedly but incompletely inhibited by 7-methylguanosine 5'-monophosphate (m7G5'p). We have analyzed the translation products synthesized in the presence of different concentrations of m7G5'p and find that translation of all mRNAs is equally inhibited. To demonstrate the specificity of the inhibitor for RNAs with 5'-terminal m7G5' ppp... we show that specific translation products of satellite tobacco necrosis virus RNA, which does not have this 5' terminus, are synthesized in the presence of m7G5' p. Protein synthesis programmed by endogenous mRNA in a HeLa cell-free system is inhibited after a 10-min lag by m7G5' p. Other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position are not inhibitor. We show that translation of all mRNAs is inhibited to a similar extent by m7G5'p in the HeLa cell-free system, by synthesizing 35S-labeled proteins in the presence of different inhibitory concentrations of this nucleotide and analyzing the translation products by electrophoresis and autoradiography. Translation of encephalomyocarditis virus RNA added to the HeLa cell-free system is not inhibited by m7\"g5p; this viral RNA does not have this nucleotide at the 5' terminus. This indicates that m7G5'p specifically inhibits translation of mRNAs with the 5' terminus m7G5'ppp... and suggests that initiation of translation of picornavirus RNA may proceed via a mechanism different from that of cellular mRNAs.", "contents": "Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate. Translation of HeLa cell RNA containing poly(A) in a wheat germ cell-free system is markedly but incompletely inhibited by 7-methylguanosine 5'-monophosphate (m7G5'p). We have analyzed the translation products synthesized in the presence of different concentrations of m7G5'p and find that translation of all mRNAs is equally inhibited. To demonstrate the specificity of the inhibitor for RNAs with 5'-terminal m7G5' ppp... we show that specific translation products of satellite tobacco necrosis virus RNA, which does not have this 5' terminus, are synthesized in the presence of m7G5' p. Protein synthesis programmed by endogenous mRNA in a HeLa cell-free system is inhibited after a 10-min lag by m7G5' p. Other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position are not inhibitor. We show that translation of all mRNAs is inhibited to a similar extent by m7G5'p in the HeLa cell-free system, by synthesizing 35S-labeled proteins in the presence of different inhibitory concentrations of this nucleotide and analyzing the translation products by electrophoresis and autoradiography. Translation of encephalomyocarditis virus RNA added to the HeLa cell-free system is not inhibited by m7\"g5p; this viral RNA does not have this nucleotide at the 5' terminus. This indicates that m7G5'p specifically inhibits translation of mRNAs with the 5' terminus m7G5'ppp... and suggests that initiation of translation of picornavirus RNA may proceed via a mechanism different from that of cellular mRNAs.", "PMID": 965382}
{"id": "pubmed23n0032_6318", "title": "Carbamylation of aspartate transaminase and the pK value of the active site lysyl residue.", "content": "Abnormal lysyl residues can be detected in aspartate transaminase by following the rate of reaction of amino groups with KN14CO and the rate of enzymatic inactivation. Peptide isolation subsequent to carbamylation of the apoenzyme produces a peptide which is absent in the carbamylated holoenzyme. The composition of the carbamylated peptide matches that of a tryptic peptide containing the active site Lys-258. The holoenzyme retains full catalytic activity after carbamylation of its NH2-terminal alanine and lysyl residues other than Lys-258, which is protected by aldimine formation with pyridoxal phosphate. Apoenzyme prepared from KNCO-treated holoenzyme (apoenzyme') is susceptible to further carbamylation at Lys-258 with irreversible loss of catalytic activity. Carbamylation of the active site lysyl residue is 25 to 50 times more rapid than that of the other 18 lysyl residues of aspartate transaminase. The kinetics of inactivation by KNCO at different pH values served to determine the pH-independent second order rate constant (k) and the pK of the amino group of Lys-258. These values are pK = 7.98 +/- 0.08 and k = 146 +/- 5 M-1S-1, which are similar to the values determined for carbamylation of the NH2- terminal groups of human hemoglobin (Garner, M. H., Bogardt, R. A., and Gurd, E. R. N. (1975) J. Biol. Chem. 250, 4398-4404). The pK value for Lys-258 is as low as that for a group in the active site region which can perturb a 19F nuclear magnetic resonance probe inserted into that region (Martinez-Carrion, M., Slebe, J. C., Boettcher, B., and Relimpio, A. M. (1976) J. Biol. Chem. 251, 1853-1858). Apoenzyme carbamylated at Lys-258 can accept pyridoxal phosphate at the active site even though no Schiff base in formed. Furthermore, this active site carbamylated holoenzyme will form spectroscopically detectable enzyme-substrate complexes with amino acids. The complexes slowly convert to species with absorbance identical with that of enzyme in the pyridoxamine phosphate form.", "contents": "Carbamylation of aspartate transaminase and the pK value of the active site lysyl residue. Abnormal lysyl residues can be detected in aspartate transaminase by following the rate of reaction of amino groups with KN14CO and the rate of enzymatic inactivation. Peptide isolation subsequent to carbamylation of the apoenzyme produces a peptide which is absent in the carbamylated holoenzyme. The composition of the carbamylated peptide matches that of a tryptic peptide containing the active site Lys-258. The holoenzyme retains full catalytic activity after carbamylation of its NH2-terminal alanine and lysyl residues other than Lys-258, which is protected by aldimine formation with pyridoxal phosphate. Apoenzyme prepared from KNCO-treated holoenzyme (apoenzyme') is susceptible to further carbamylation at Lys-258 with irreversible loss of catalytic activity. Carbamylation of the active site lysyl residue is 25 to 50 times more rapid than that of the other 18 lysyl residues of aspartate transaminase. The kinetics of inactivation by KNCO at different pH values served to determine the pH-independent second order rate constant (k) and the pK of the amino group of Lys-258. These values are pK = 7.98 +/- 0.08 and k = 146 +/- 5 M-1S-1, which are similar to the values determined for carbamylation of the NH2- terminal groups of human hemoglobin (Garner, M. H., Bogardt, R. A., and Gurd, E. R. N. (1975) J. Biol. Chem. 250, 4398-4404). The pK value for Lys-258 is as low as that for a group in the active site region which can perturb a 19F nuclear magnetic resonance probe inserted into that region (Martinez-Carrion, M., Slebe, J. C., Boettcher, B., and Relimpio, A. M. (1976) J. Biol. Chem. 251, 1853-1858). Apoenzyme carbamylated at Lys-258 can accept pyridoxal phosphate at the active site even though no Schiff base in formed. Furthermore, this active site carbamylated holoenzyme will form spectroscopically detectable enzyme-substrate complexes with amino acids. The complexes slowly convert to species with absorbance identical with that of enzyme in the pyridoxamine phosphate form.", "PMID": 965383}
{"id": "pubmed23n0032_6319", "title": "Solubilization of nerve growth factor receptors of rabbit superior cervical ganglia.", "content": "Nerve growth factor (NGF) receptors of rabbit superior cervical ganglia can be solubilized by treatment with detergents and readily assayed in the soluble state. Triton X-100 and deoxycholate reduce specific binding of NGF to ganglia membranes. In membranes treated with Triton X-100 (0.5 to 2.0%) the reduction in NGF binding by membranes is accompanied by a corresponding increase in binding in the supernatant fluid. NGF binding in soluble preparations can be rapidly assayed by precipitating NGF bound to receptors with polyethylene glycol under conditions in which unbound NGF is not precipitated. NGF binding to soluble preparations is saturable whether evaluated by the binding of 125I-NGF or by diluting 125I-NGF with native NGF. Using both techniques, the dissociation constant for NGF binding to soluble receptors is about 0.2 nM, the same as its dissociation constant from receptor sites in intact membranes. NGF binding to soluble receptors displays a high degree of peptide specificity, similar to receptor sites in intact membranes of superior cervical ganglia. A method of labeling NGF with 125I-3(4-hydroxyphenyl) propionic acid N-hydroxysuccinimide ester is described which leads to binding properties that are superior to those obtained with previously described 125I-NGF preparations.", "contents": "Solubilization of nerve growth factor receptors of rabbit superior cervical ganglia. Nerve growth factor (NGF) receptors of rabbit superior cervical ganglia can be solubilized by treatment with detergents and readily assayed in the soluble state. Triton X-100 and deoxycholate reduce specific binding of NGF to ganglia membranes. In membranes treated with Triton X-100 (0.5 to 2.0%) the reduction in NGF binding by membranes is accompanied by a corresponding increase in binding in the supernatant fluid. NGF binding in soluble preparations can be rapidly assayed by precipitating NGF bound to receptors with polyethylene glycol under conditions in which unbound NGF is not precipitated. NGF binding to soluble preparations is saturable whether evaluated by the binding of 125I-NGF or by diluting 125I-NGF with native NGF. Using both techniques, the dissociation constant for NGF binding to soluble receptors is about 0.2 nM, the same as its dissociation constant from receptor sites in intact membranes. NGF binding to soluble receptors displays a high degree of peptide specificity, similar to receptor sites in intact membranes of superior cervical ganglia. A method of labeling NGF with 125I-3(4-hydroxyphenyl) propionic acid N-hydroxysuccinimide ester is described which leads to binding properties that are superior to those obtained with previously described 125I-NGF preparations.", "PMID": 965384}
{"id": "pubmed23n0032_6320", "title": "Purification and characterization of a lectin from Wistaria floribunda seeds.", "content": "A lectin from Wistaria floribunda seeds which specifically binds to N-acetyl-D-galactosamine was purified to homogeneity as judged by polyacrylamide gel electrophoresis. Its molecular weight was estimated to be 68,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. It dissociated into subunits on reduction with 2-mercaptoethanol with concomitant loss of hemagglutinating activity. On oxidation in air, the subunits reassociated into the lectin molecules with hemagglutinating activity. Carboxymethylation of the subunits with iodoacetic acid prevented their reassociation on oxidation in air. The molecular weight of the subunits was 32,000, which is about one-half that of the native lectin, suggesting that the lectin consists of two subunits. The results total, NH2-terminal, and COOH-terminal amino acid analyses, and mapping of the tryptic digest of the lectin indicated that these two subunits were indistinguishable and were probably identical, and that they were linked together covalently through a single disulfide bond. Equilibrium dialysis experiments show that the lectin and its subunit molecules are divalent and monovalent, respectively, with respect to sugar binding. The lectin is a glycoprotein, containing 3.2% carbohydrate. The carbohydrate moiety is composed of mannose, galactose, and glucosamine is a molar ratio of 1:2:1 and these sugars seem to be linked as a single oligosaccharide chain to each subunit of the protein.", "contents": "Purification and characterization of a lectin from Wistaria floribunda seeds. A lectin from Wistaria floribunda seeds which specifically binds to N-acetyl-D-galactosamine was purified to homogeneity as judged by polyacrylamide gel electrophoresis. Its molecular weight was estimated to be 68,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. It dissociated into subunits on reduction with 2-mercaptoethanol with concomitant loss of hemagglutinating activity. On oxidation in air, the subunits reassociated into the lectin molecules with hemagglutinating activity. Carboxymethylation of the subunits with iodoacetic acid prevented their reassociation on oxidation in air. The molecular weight of the subunits was 32,000, which is about one-half that of the native lectin, suggesting that the lectin consists of two subunits. The results total, NH2-terminal, and COOH-terminal amino acid analyses, and mapping of the tryptic digest of the lectin indicated that these two subunits were indistinguishable and were probably identical, and that they were linked together covalently through a single disulfide bond. Equilibrium dialysis experiments show that the lectin and its subunit molecules are divalent and monovalent, respectively, with respect to sugar binding. The lectin is a glycoprotein, containing 3.2% carbohydrate. The carbohydrate moiety is composed of mannose, galactose, and glucosamine is a molar ratio of 1:2:1 and these sugars seem to be linked as a single oligosaccharide chain to each subunit of the protein.", "PMID": 965385}
{"id": "pubmed23n0032_6321", "title": "Studies on the role of plasminogen activator in ovulation. In vitro response of granulosa cells to gonadotropins, cyclic nucleotides, and prostaglandins.", "content": "A quantitative method is described for measuring the amount of plasminogen activator produced by rat ovarian granulosa cells following exposure to hormones in vivo or in vitro. The results confirm the previously reported observation (Beers, W. H., Strickland, S., and Reich, E. (1975) Cell 6, (387-394) that granulosa cells in vivo produce increasing amounts of plasminogen activator as the time of ovulation approaches and that the enzyme is produced only be cells obtained from follicles destined to ovulate. Inactive cells can be stimulated in vitro by gonadotropins to produce plasminogen activator. This response is time- and dose-dependent, and results in an increase of intracellular and extracellular enzyme. Studies of the specificity of this response indicate that preparations of follicle-stimulating hormone are much more effective than corresponding preparations of luteinizing hormone. The effect of other pituitary hormones is also presented. Molecules other than gonadotropins are also capable of stimulating the cells to produce the enzyme. Prostaglandins E1 and E2 and analogues of cAMP effectively stimulated the cells to produce plasminogen activator, cGMP and its analogues and prostaglandins F1a and F2a were without effect as were the six steroids studied. The inactive compounds also did not inhibit the response of the cells to gonadotropins. The granulosa cell plasminogen activator has been analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has an apparent molecular weight of 75,000. By this and other criteria, the granulosa cell enzyme is similar to one of the species of plasminogen activators obtained from cultures of simian virus 40-transformed rat embryo fibroblasts.", "contents": "Studies on the role of plasminogen activator in ovulation. In vitro response of granulosa cells to gonadotropins, cyclic nucleotides, and prostaglandins. A quantitative method is described for measuring the amount of plasminogen activator produced by rat ovarian granulosa cells following exposure to hormones in vivo or in vitro. The results confirm the previously reported observation (Beers, W. H., Strickland, S., and Reich, E. (1975) Cell 6, (387-394) that granulosa cells in vivo produce increasing amounts of plasminogen activator as the time of ovulation approaches and that the enzyme is produced only be cells obtained from follicles destined to ovulate. Inactive cells can be stimulated in vitro by gonadotropins to produce plasminogen activator. This response is time- and dose-dependent, and results in an increase of intracellular and extracellular enzyme. Studies of the specificity of this response indicate that preparations of follicle-stimulating hormone are much more effective than corresponding preparations of luteinizing hormone. The effect of other pituitary hormones is also presented. Molecules other than gonadotropins are also capable of stimulating the cells to produce the enzyme. Prostaglandins E1 and E2 and analogues of cAMP effectively stimulated the cells to produce plasminogen activator, cGMP and its analogues and prostaglandins F1a and F2a were without effect as were the six steroids studied. The inactive compounds also did not inhibit the response of the cells to gonadotropins. The granulosa cell plasminogen activator has been analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has an apparent molecular weight of 75,000. By this and other criteria, the granulosa cell enzyme is similar to one of the species of plasminogen activators obtained from cultures of simian virus 40-transformed rat embryo fibroblasts.", "PMID": 965386}
{"id": "pubmed23n0032_6322", "title": "Nonuniform biosynthesis of multiple hemoglobins in the adult rat and guinea pig.", "content": "Separation of adult rat bone marrow cells by the method of thin layer countercurrent distribution permits the analyses of 59Fe-tagged erythroid cells for the various multiple hemoglobins and the assignment of such hemoglobins to erythroid cells at different stages of their development. Of the six adult red cell hemoglobins, hemoglobin 5 is synthesized most actively in the earliest erythroid cell whereas hemoglobin 4 (the major hemoglobin of the red cell) is synthesized most actively in the latest erythroid cells, e.g. the reticulocyte. Experimental evidence also indicates that maturation of the erythroid cell is accompanied by a decreased rate of synthesis of hemoglobin 5. The earliest erythroid cells of the marrow contain two hemoglobins, 7 and 8, which are absent in the adult red cell. Similar studies with the guinea pig confirm the nonuniform biosynthesis of its two hemoglobins and suggest that the phenomenon may be a general one among mammalian multiple hemoglobins.", "contents": "Nonuniform biosynthesis of multiple hemoglobins in the adult rat and guinea pig. Separation of adult rat bone marrow cells by the method of thin layer countercurrent distribution permits the analyses of 59Fe-tagged erythroid cells for the various multiple hemoglobins and the assignment of such hemoglobins to erythroid cells at different stages of their development. Of the six adult red cell hemoglobins, hemoglobin 5 is synthesized most actively in the earliest erythroid cell whereas hemoglobin 4 (the major hemoglobin of the red cell) is synthesized most actively in the latest erythroid cells, e.g. the reticulocyte. Experimental evidence also indicates that maturation of the erythroid cell is accompanied by a decreased rate of synthesis of hemoglobin 5. The earliest erythroid cells of the marrow contain two hemoglobins, 7 and 8, which are absent in the adult red cell. Similar studies with the guinea pig confirm the nonuniform biosynthesis of its two hemoglobins and suggest that the phenomenon may be a general one among mammalian multiple hemoglobins.", "PMID": 965387}
{"id": "pubmed23n0032_6323", "title": "Requirement for homologous rabbit reticulocyte initiation factor 3 for initiation of alpha- and beta-globin mRNA translation in a crude protozoal cell-free system.", "content": "Experimental evidence showing specificity of rabbit reticulocyte initiation factor 3 (EIF-3) for selective initiation of mRNA translation is presented. A new cell-free system was developed from Crithidia fasciculata. The crude postmitochondrial supernatant fluid was treated with puromycin and 0.5 M KCl to dissociate mRNA from polysomes and ribosomes into subunits. The drug and salt were removed by gel filtration on Sephadex G-25. Additions of amino acids and energy source initiate protein synthesis. All synthesis starts at the initiation site. This treatment brought about a shift in MgCl2 optimum from 6 to 3 mM. Exogenously supplied rabbit reticulocyte globin mRNA is faithfully translated in this system. However, crithidial EIF-3 has a low affinity for globin mRNA as evidenced by a 6-fold increase in the rate of globin synthesis after the addition of rabbit reticulocyte EIF-3 in the range at which globin synthesis is linear to the amount of globin mRNA added to the system. It is also shown that in a reconstituted system in which ribosomal subunits are depleted from initiation factors, EIF-3 from rabbit reticulocytes has a higher affinity for globin mRNA, as measured by the formation of polysomes during the linear time of amino acid incorporation. These results are taken to indicate that initiation factor EIF-3 action should be considered as an enzyme catalyzed reaction for which various mRNAs serve as different substrate analogs. Therefore, specificity is most likely to be expressed as an affinity of enzyme to substrate and would show as rate difference rather than an all-or-none phenomenon.", "contents": "Requirement for homologous rabbit reticulocyte initiation factor 3 for initiation of alpha- and beta-globin mRNA translation in a crude protozoal cell-free system. Experimental evidence showing specificity of rabbit reticulocyte initiation factor 3 (EIF-3) for selective initiation of mRNA translation is presented. A new cell-free system was developed from Crithidia fasciculata. The crude postmitochondrial supernatant fluid was treated with puromycin and 0.5 M KCl to dissociate mRNA from polysomes and ribosomes into subunits. The drug and salt were removed by gel filtration on Sephadex G-25. Additions of amino acids and energy source initiate protein synthesis. All synthesis starts at the initiation site. This treatment brought about a shift in MgCl2 optimum from 6 to 3 mM. Exogenously supplied rabbit reticulocyte globin mRNA is faithfully translated in this system. However, crithidial EIF-3 has a low affinity for globin mRNA as evidenced by a 6-fold increase in the rate of globin synthesis after the addition of rabbit reticulocyte EIF-3 in the range at which globin synthesis is linear to the amount of globin mRNA added to the system. It is also shown that in a reconstituted system in which ribosomal subunits are depleted from initiation factors, EIF-3 from rabbit reticulocytes has a higher affinity for globin mRNA, as measured by the formation of polysomes during the linear time of amino acid incorporation. These results are taken to indicate that initiation factor EIF-3 action should be considered as an enzyme catalyzed reaction for which various mRNAs serve as different substrate analogs. Therefore, specificity is most likely to be expressed as an affinity of enzyme to substrate and would show as rate difference rather than an all-or-none phenomenon.", "PMID": 965388}
{"id": "pubmed23n0032_6324", "title": "Interaction of diphtheria toxin with mammalian cell membranes.", "content": "Uptake of 125I-labeled diphtheria toxin and serologically related proteins by a sensitive human HeLa cell line and by a resistant mouse L929 cell line has been studied. The evidence suggests that there is an initial rapid reaction between a recognition site present on the toxin Fragment B and specific plasma membrane receptors on the sensitive cell (there are approximately 4000/HeLa cell). This initial interaction is followed by a slow irreversible process during which there is a major conformational alteration of the toxin molecule causing the enzymically active 22,000-dalton Fragment A to become exposed to the cytosol. We suggest that it is at this point that cleavage of the NH2-terminal disulfide bond occurs leading to release of Fragment A into the cytoplasm. The toxin Fragment B remains attached to the membrane, probably formed in a complex with receptor, and blocks entry of additional toxin molecules through the same site. Specific membrane receptors are lacking from mouse cells. Both HeLa cells and L929 cells internalize toxin, related nontoxic proteins, and inert molecules such as inulin nonspecifically into endocytotoc vesicles. At 30 degrees the bulk internalization of extracellular fluid is about 1.2% of their cell volume per h for both cell lines. Fragment A does not traverse the plasma membrane by a mechanism that depends on endocytosis. The interaction of diphtheria toxin with the sensitive cell membrane is discussed in relation to other protein toxins and certain glycopeptide tropic hormones in which relatively large, hydrophilic polypeptide fragments or subunits are presumed to traverse the target cell plasma membrane and reach the cytoplasm in biologically active form.", "contents": "Interaction of diphtheria toxin with mammalian cell membranes. Uptake of 125I-labeled diphtheria toxin and serologically related proteins by a sensitive human HeLa cell line and by a resistant mouse L929 cell line has been studied. The evidence suggests that there is an initial rapid reaction between a recognition site present on the toxin Fragment B and specific plasma membrane receptors on the sensitive cell (there are approximately 4000/HeLa cell). This initial interaction is followed by a slow irreversible process during which there is a major conformational alteration of the toxin molecule causing the enzymically active 22,000-dalton Fragment A to become exposed to the cytosol. We suggest that it is at this point that cleavage of the NH2-terminal disulfide bond occurs leading to release of Fragment A into the cytoplasm. The toxin Fragment B remains attached to the membrane, probably formed in a complex with receptor, and blocks entry of additional toxin molecules through the same site. Specific membrane receptors are lacking from mouse cells. Both HeLa cells and L929 cells internalize toxin, related nontoxic proteins, and inert molecules such as inulin nonspecifically into endocytotoc vesicles. At 30 degrees the bulk internalization of extracellular fluid is about 1.2% of their cell volume per h for both cell lines. Fragment A does not traverse the plasma membrane by a mechanism that depends on endocytosis. The interaction of diphtheria toxin with the sensitive cell membrane is discussed in relation to other protein toxins and certain glycopeptide tropic hormones in which relatively large, hydrophilic polypeptide fragments or subunits are presumed to traverse the target cell plasma membrane and reach the cytoplasm in biologically active form.", "PMID": 965389}
{"id": "pubmed23n0032_6325", "title": "Stimulation of prostaglandin synthesis by bradykinin and thrombin and their mechanisms of action on MC5-5 fibroblasts.", "content": "Transformed mouse fibroblasts, in which [3H]arachidonic acid was incorporated in the cellular lipids, released radioactive materials into the medium when stimulated by bradykinin and thrombin. Most of this released radioactivity was found in the landins; the remainder was present in the arachidonic acid fraction. When the cells stimulated by serum or when they were mechanically manipulated, not only prostaglandins but also high levels of arachidonic acid were released. Anti-inflammatory steroids, which decrease the availability of arachidonic acid, inhibited the stimulated release of prostaglandins caused by the vasoactive agents, suggesting that bradykinin and thrombin stimulated prostaglandin synthesis by increasing deacylation of the phospholipids. The efficiency of the conversion of the released arachidonic acid into prostaglandins appears to reflect the cellular proximity of the phospholipid deacylating activity and prostaglandin synthesizing system.", "contents": "Stimulation of prostaglandin synthesis by bradykinin and thrombin and their mechanisms of action on MC5-5 fibroblasts. Transformed mouse fibroblasts, in which [3H]arachidonic acid was incorporated in the cellular lipids, released radioactive materials into the medium when stimulated by bradykinin and thrombin. Most of this released radioactivity was found in the landins; the remainder was present in the arachidonic acid fraction. When the cells stimulated by serum or when they were mechanically manipulated, not only prostaglandins but also high levels of arachidonic acid were released. Anti-inflammatory steroids, which decrease the availability of arachidonic acid, inhibited the stimulated release of prostaglandins caused by the vasoactive agents, suggesting that bradykinin and thrombin stimulated prostaglandin synthesis by increasing deacylation of the phospholipids. The efficiency of the conversion of the released arachidonic acid into prostaglandins appears to reflect the cellular proximity of the phospholipid deacylating activity and prostaglandin synthesizing system.", "PMID": 965390}
{"id": "pubmed23n0032_6326", "title": "Mechanism for enzymatic thioether formation. Mechanism of action of S-adenosylhomocysteinase.", "content": "Homogeneous S-adenosylhomocysteinase contains tightly bound NAD+. This NAD+ is not dissociable under nondenaturing conditions but can be removed by heat or acid denaturation. Addition of adenosine to the enzyme causes an increase in the absorption at 327 nm which we attribute to NADH formation. The enzyme also catalyzes the rapid exchange of the adenosine 4'-proton with solvent water. A mechanism is proposed for the reversible hydrolysis of S-adenosyl-L-homocysteine involving oxidation of position 3' of adenosine followed by alpha-beta elimination of L-homocysteine to give 3'-deto-4',5'-dehydro-5'-deoxyadenosine. This compound reacts with water in a Michael-type addition to form 3'-keto adenosine which is then re-reduced to adenosine.", "contents": "Mechanism for enzymatic thioether formation. Mechanism of action of S-adenosylhomocysteinase. Homogeneous S-adenosylhomocysteinase contains tightly bound NAD+. This NAD+ is not dissociable under nondenaturing conditions but can be removed by heat or acid denaturation. Addition of adenosine to the enzyme causes an increase in the absorption at 327 nm which we attribute to NADH formation. The enzyme also catalyzes the rapid exchange of the adenosine 4'-proton with solvent water. A mechanism is proposed for the reversible hydrolysis of S-adenosyl-L-homocysteine involving oxidation of position 3' of adenosine followed by alpha-beta elimination of L-homocysteine to give 3'-deto-4',5'-dehydro-5'-deoxyadenosine. This compound reacts with water in a Michael-type addition to form 3'-keto adenosine which is then re-reduced to adenosine.", "PMID": 965391}
{"id": "pubmed23n0032_6327", "title": "Determination of the michaelis-menten constant for beta-hydroxy-beta-methylglutaryl coenzyme A reductase. Demonstration of a substrate affinity 10-fold greater than previously reported.", "content": "This paper presents evidence that earlier kinetic determinations of rat liver beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase, the reported rate-determining enzyme in cholesterol biogenesis, may have substantially underestimated the affinity of this enzyme for HMG-CoA. Nonlinear conversion of this substrate to mevalonic acid, with time, is shown under the conditions of a previous study, and it was concluded that velocities were limited at the lower substrate levels tested by an insufficient quantity of substrate. For this present study, conditions were established under which linearity of time versus product formation was observed, and the extent of conversion did not exceed 6% of the initial quantity of D isomer. These conditions gave a Michaelis-Menten constant of 1.01 +/- 0.12 muM for the D isomer, which is 3.5 to 40 times lower than those previously reported.", "contents": "Determination of the michaelis-menten constant for beta-hydroxy-beta-methylglutaryl coenzyme A reductase. Demonstration of a substrate affinity 10-fold greater than previously reported. This paper presents evidence that earlier kinetic determinations of rat liver beta-hydroxy-beta-methylglutaryl coenzyme A (HMG-CoA) reductase, the reported rate-determining enzyme in cholesterol biogenesis, may have substantially underestimated the affinity of this enzyme for HMG-CoA. Nonlinear conversion of this substrate to mevalonic acid, with time, is shown under the conditions of a previous study, and it was concluded that velocities were limited at the lower substrate levels tested by an insufficient quantity of substrate. For this present study, conditions were established under which linearity of time versus product formation was observed, and the extent of conversion did not exceed 6% of the initial quantity of D isomer. These conditions gave a Michaelis-Menten constant of 1.01 +/- 0.12 muM for the D isomer, which is 3.5 to 40 times lower than those previously reported.", "PMID": 965392}
{"id": "pubmed23n0032_6328", "title": "Inhibition of oxalacetate decarboxylation during C4 photosynthesis by 3-mercaptopicolin acid.", "content": "3-Mercaptopicolinic acid specifically inhibits phosphoenolpyruvate carboxykinase in leaves of the C4 plant Panicum maximum. Both the ATP- and ADP-dependent decarboxylation of oxalacetate and the carboxylation activity of phosphoenolpyruvate carboxykinase are inhibited by 3-mercaptopicolinic acid while phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase are not inhibited. 3-Mercaptopicolinic acid inhibits the fixation of 14CO2 by illuminated P. maximum bundle sheath strands which is dependent upon oxalacetate and ATP but does not affect C3 photosynthesis in bundle sheath strands nor C4 photosynthesis in mesophyll cells. 3-Mercaptopicolinic acid treatment reduced P. maximum leaf photosynthesis 25% while raising the photosynthetic CO2 compensation point from near zero to 18 to 45 mul of CO2/liter of air.", "contents": "Inhibition of oxalacetate decarboxylation during C4 photosynthesis by 3-mercaptopicolin acid. 3-Mercaptopicolinic acid specifically inhibits phosphoenolpyruvate carboxykinase in leaves of the C4 plant Panicum maximum. Both the ATP- and ADP-dependent decarboxylation of oxalacetate and the carboxylation activity of phosphoenolpyruvate carboxykinase are inhibited by 3-mercaptopicolinic acid while phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase are not inhibited. 3-Mercaptopicolinic acid inhibits the fixation of 14CO2 by illuminated P. maximum bundle sheath strands which is dependent upon oxalacetate and ATP but does not affect C3 photosynthesis in bundle sheath strands nor C4 photosynthesis in mesophyll cells. 3-Mercaptopicolinic acid treatment reduced P. maximum leaf photosynthesis 25% while raising the photosynthetic CO2 compensation point from near zero to 18 to 45 mul of CO2/liter of air.", "PMID": 965393}
{"id": "pubmed23n0032_6329", "title": "Vitamin K-dependent carboxylase. Development of a peptide substrate.", "content": "Rat liver microsomes contain a vitamin K-dependent carboxylase activity that converts specific glutamyl residues of microsomal prothrombin precursor to gamma-carboxyglutamic acid residues. This activity has now been solubilized by treatment with Triton X-100. The pentapeptide, Phe-Leu-Glu-Glu-Val, has been synthesized; and it has been demonstrated that, in the presence of this peptide, the solubilized microsomes catalyze a vitamin K-dependent incorporation of added H14CO3- into a low molecular weight trichloroacetic acid-soluble compound. The carboxylated product has been identified as peptide-bound gamma-carboxyglutamic acid by its chemical stability during acidic and alkaline hydrolysis and by co-chromatography of an alkaline hydrolysate of the product with authentic gamma-carboxyglutamic acid. The conditions for peptide carboxylation appear to be identical with those demonstrated for precursor carboxylation.", "contents": "Vitamin K-dependent carboxylase. Development of a peptide substrate. Rat liver microsomes contain a vitamin K-dependent carboxylase activity that converts specific glutamyl residues of microsomal prothrombin precursor to gamma-carboxyglutamic acid residues. This activity has now been solubilized by treatment with Triton X-100. The pentapeptide, Phe-Leu-Glu-Glu-Val, has been synthesized; and it has been demonstrated that, in the presence of this peptide, the solubilized microsomes catalyze a vitamin K-dependent incorporation of added H14CO3- into a low molecular weight trichloroacetic acid-soluble compound. The carboxylated product has been identified as peptide-bound gamma-carboxyglutamic acid by its chemical stability during acidic and alkaline hydrolysis and by co-chromatography of an alkaline hydrolysate of the product with authentic gamma-carboxyglutamic acid. The conditions for peptide carboxylation appear to be identical with those demonstrated for precursor carboxylation.", "PMID": 965394}
{"id": "pubmed23n0032_6330", "title": "Two soluble forms of guanosine 5'-(beta,gamma-imino)triphosphate and fluoride-activated adenylate cyclase.", "content": "Soluble and membrane-bound adenylate cyclase from the canine renal medulla are very slowly activated by guanosine 5'-(beta,gamma-imino)triphosphate (Gpp(NH)p) or fluoride. At 25 degrees, 8 to 10 h of incubation of the soluble enzyme, and 6 to 8 h of incubation of the membrane-bound enzyme are required to reach the maximal activity. The dependence of activation on concentration of Gpp(NH)p changes with time. Half-maximal activation of soluble adenylate cyclase occurs at 3 +/- 1 X 10(-6) M Gpp(NH)p if the activity is measured without preincubation with Gpp(NH)pp and at 4 +/- 2 X 10(-8) M if it is measured after 6 to 22 h of incubation with Gpp(NH)p at 25 degrees. The activation occurs over a rather broad range of nucleotide concentration and cannot, therefore, be simply interpreted to reflect a function of nucleotide binding. Two forms of soluble adenylate cyclase are resolved by Sepharose gel filtration. One form, with a Stokes radius of 71 A, is rapidly activated by Gpp(NH)p; the other, with a Stokes radius of 56 A, requires long incubation with Gpp(NH)p to be activated. The sedimentation coefficient of the two forms is 7.3 S. The apparent molecular weight of the rapidly activated form is 200,000 while that of the slowly activated form is 160,000. The interrelationship of these two species of adenylate cyclase is not clear and is under investigation.", "contents": "Two soluble forms of guanosine 5'-(beta,gamma-imino)triphosphate and fluoride-activated adenylate cyclase. Soluble and membrane-bound adenylate cyclase from the canine renal medulla are very slowly activated by guanosine 5'-(beta,gamma-imino)triphosphate (Gpp(NH)p) or fluoride. At 25 degrees, 8 to 10 h of incubation of the soluble enzyme, and 6 to 8 h of incubation of the membrane-bound enzyme are required to reach the maximal activity. The dependence of activation on concentration of Gpp(NH)p changes with time. Half-maximal activation of soluble adenylate cyclase occurs at 3 +/- 1 X 10(-6) M Gpp(NH)p if the activity is measured without preincubation with Gpp(NH)pp and at 4 +/- 2 X 10(-8) M if it is measured after 6 to 22 h of incubation with Gpp(NH)p at 25 degrees. The activation occurs over a rather broad range of nucleotide concentration and cannot, therefore, be simply interpreted to reflect a function of nucleotide binding. Two forms of soluble adenylate cyclase are resolved by Sepharose gel filtration. One form, with a Stokes radius of 71 A, is rapidly activated by Gpp(NH)p; the other, with a Stokes radius of 56 A, requires long incubation with Gpp(NH)p to be activated. The sedimentation coefficient of the two forms is 7.3 S. The apparent molecular weight of the rapidly activated form is 200,000 while that of the slowly activated form is 160,000. The interrelationship of these two species of adenylate cyclase is not clear and is under investigation.", "PMID": 965395}
{"id": "pubmed23n0032_6331", "title": "A comparison of membrane fracture faces of fixed and unfixed glycerinated tissue.", "content": "Fixed (glutaraldehyde, 3%) and unfixed specimens of rat buccal epithelium, striated muscle, and liver, were cryoprotected with glycerol, freeze-fractured, and replicated without sublimation. A comparison of fracture faces of general plasma membranes, nuclear membranes, mitochondrial membranes, and membranes of rough endoplasmic reticulum revealed no significant differences as between fixed and unfixed material. Apart from some membranes of liver endoplasmic reticulum, there was no evidence of aggregation or redistribution of intramembranous particles in the unfixed material. The results demonstrate that chemical prefixation of tissues for freeze-fracture is not always necessary, or even desirable, and that glycerol may not be as deeply or directly implicated in particle aggregation as previously thought. Fixation with glutaraldehyde alters the cleaving behaviour of plasma membrane at desmosomes and tight junctions, but not at gap junctions.", "contents": "A comparison of membrane fracture faces of fixed and unfixed glycerinated tissue. Fixed (glutaraldehyde, 3%) and unfixed specimens of rat buccal epithelium, striated muscle, and liver, were cryoprotected with glycerol, freeze-fractured, and replicated without sublimation. A comparison of fracture faces of general plasma membranes, nuclear membranes, mitochondrial membranes, and membranes of rough endoplasmic reticulum revealed no significant differences as between fixed and unfixed material. Apart from some membranes of liver endoplasmic reticulum, there was no evidence of aggregation or redistribution of intramembranous particles in the unfixed material. The results demonstrate that chemical prefixation of tissues for freeze-fracture is not always necessary, or even desirable, and that glycerol may not be as deeply or directly implicated in particle aggregation as previously thought. Fixation with glutaraldehyde alters the cleaving behaviour of plasma membrane at desmosomes and tight junctions, but not at gap junctions.", "PMID": 965426}
{"id": "pubmed23n0032_6332", "title": "Intranuclear electrophoresis of the chromatin of living cells.", "content": "Intranuclear electrophoresis of living cells under appropriate conditions causes the chromatin and nucleoli to move rapidly into the anodal side of the nucleus. In pig kidney cells, chromatin lengths attached to the nuclear envelope are oriented by the current and freed from surrounding non-oriented chromatin. Individual chromatin strands isolated in this way are often long and have not been subjected to the trauma of isolation from the nucleus. This has allowed us to demonstrate oriented lines of up to 8 chromomeres in a strand, linked by fine single fibres. These chromomeres of chromatin have the same linear dimensions as the bands and interbands of polytene chromosomes. A very wide range of morphology of chromatin is revealed - from lines of nucleosomes in open array, to strands uniformly 25 nm thick. Doublet strands and multiple strands - often embedded in darkly staining material - are also seen. All morphological types may be seen in the same nucleus. Many of the oriented threads appear to be transcriptionally active. The variable morphology of these sites and their relation to peripheral heterochromatin is discussed. Histone nucleosomes are present in these apparently transcriptionally active regions. The method is useful for investigating the relationships between chromatin and the nuclear envelope. Approximately 1500 attachment sites per nucleus are found in these cells. Some nucleoli are attached to the nuclear envelope.", "contents": "Intranuclear electrophoresis of the chromatin of living cells. Intranuclear electrophoresis of living cells under appropriate conditions causes the chromatin and nucleoli to move rapidly into the anodal side of the nucleus. In pig kidney cells, chromatin lengths attached to the nuclear envelope are oriented by the current and freed from surrounding non-oriented chromatin. Individual chromatin strands isolated in this way are often long and have not been subjected to the trauma of isolation from the nucleus. This has allowed us to demonstrate oriented lines of up to 8 chromomeres in a strand, linked by fine single fibres. These chromomeres of chromatin have the same linear dimensions as the bands and interbands of polytene chromosomes. A very wide range of morphology of chromatin is revealed - from lines of nucleosomes in open array, to strands uniformly 25 nm thick. Doublet strands and multiple strands - often embedded in darkly staining material - are also seen. All morphological types may be seen in the same nucleus. Many of the oriented threads appear to be transcriptionally active. The variable morphology of these sites and their relation to peripheral heterochromatin is discussed. Histone nucleosomes are present in these apparently transcriptionally active regions. The method is useful for investigating the relationships between chromatin and the nuclear envelope. Approximately 1500 attachment sites per nucleus are found in these cells. Some nucleoli are attached to the nuclear envelope.", "PMID": 965427}
{"id": "pubmed23n0032_6333", "title": "Rates of synthesis of polyadenylated messenger RNA and ribosomal RNA during the cell cycle of Schizosaccharomyces pombe. With an appendix: calculation of the pattern of protein accumulation from observed changes in the rate of messenger RNA synthesis.", "content": "The rates of polyadenylated messenger RNA and ribosomal RNA synthesis were measured in synchronously dividing cultures of fission yeast (Schizosaccharomyces pombe). Control asynchronous cultures, which had been exposed to the conditions used for preparing synchronous cultures, were investigated to check for effects of the synchronization procedure itself on RNA synthesis. After each period of DNA synthesis in synchronous culture, the rates of messenger and ribosomal RNA synthesis doubled, suggesting that gene number controls the rate of messenger and ribosomal RNA synthesis. This was confirmed by experiments with asynchronous, exponential-phase cultures in which DNA synthesis was inhibited by hydroxyurea. Both synchronous culture and hydroxyurea experiments suggested that there is a delay of 15 min (0-1 of the cell generation time) between replication of the DNA and transcription of both gene copies. A pattern of protein accumulation was calculated from changes in the rate of polyadenylated messenger RNA synthesis during synchronous culture. The simulated pattern indicates that protein is accumulated linearly, with a doubling in the rate of accumulation once per cell cycle. The simulated pattern of protein accumulation is very similar to measurements previously reported by other workers of changes in activities of 3 enzymes in synchronous cultures. It is suggested that the doubling of the rate of messenger RNA synthesis, as a consequence of the replication of the DNA once per cycle, provides the basis of a mechanism for control of the doubling of other cellular constituents during the cell cycle.", "contents": "Rates of synthesis of polyadenylated messenger RNA and ribosomal RNA during the cell cycle of Schizosaccharomyces pombe. With an appendix: calculation of the pattern of protein accumulation from observed changes in the rate of messenger RNA synthesis. The rates of polyadenylated messenger RNA and ribosomal RNA synthesis were measured in synchronously dividing cultures of fission yeast (Schizosaccharomyces pombe). Control asynchronous cultures, which had been exposed to the conditions used for preparing synchronous cultures, were investigated to check for effects of the synchronization procedure itself on RNA synthesis. After each period of DNA synthesis in synchronous culture, the rates of messenger and ribosomal RNA synthesis doubled, suggesting that gene number controls the rate of messenger and ribosomal RNA synthesis. This was confirmed by experiments with asynchronous, exponential-phase cultures in which DNA synthesis was inhibited by hydroxyurea. Both synchronous culture and hydroxyurea experiments suggested that there is a delay of 15 min (0-1 of the cell generation time) between replication of the DNA and transcription of both gene copies. A pattern of protein accumulation was calculated from changes in the rate of polyadenylated messenger RNA synthesis during synchronous culture. The simulated pattern indicates that protein is accumulated linearly, with a doubling in the rate of accumulation once per cell cycle. The simulated pattern of protein accumulation is very similar to measurements previously reported by other workers of changes in activities of 3 enzymes in synchronous cultures. It is suggested that the doubling of the rate of messenger RNA synthesis, as a consequence of the replication of the DNA once per cycle, provides the basis of a mechanism for control of the doubling of other cellular constituents during the cell cycle.", "PMID": 965428}
{"id": "pubmed23n0032_6334", "title": "Proliferation and agglutinability of primary and transformed human epithelial cells in culture.", "content": "Primary epithelial populations (HAM) were obtained by dissociation of the amniotic membrane stripped from human placentae. Agglutinability of cells from such normal populations and of cells from the transformed epithelial line WISH was then compared using concavanalin A as mediator. Extensive similar studies have previously been reported with cell strains isolated from other species. Freshly dissociated HAM cells from primary cultures agglutinated much less readily than did cells from WISH populations. Furthermore, the former exhibited a drastic decline in agglutinability as a function of time in suspension culture after trypsinization. Short-term exposure (60 h) of HAM cells in monolayer culture to 5-bromodeoxyuridine (BrdU) elicited heightened agglutinability detectable through 22 days in vitro. Addition of the protease inhibitors n-tosyl-L-lysyl-chloromethyl ketone (TLCK) or p-tosyl-L-arginine-methyl ester (TAME) to the culture medium inhibited proliferation of the WISH line by 40--50% while effecting only a 10-15% inhibition of HAM cells. These results also confirm data with other cell species indicating that high proteolytic activity at the surface of transformed cells may be related to the rapid proliferation rate.", "contents": "Proliferation and agglutinability of primary and transformed human epithelial cells in culture. Primary epithelial populations (HAM) were obtained by dissociation of the amniotic membrane stripped from human placentae. Agglutinability of cells from such normal populations and of cells from the transformed epithelial line WISH was then compared using concavanalin A as mediator. Extensive similar studies have previously been reported with cell strains isolated from other species. Freshly dissociated HAM cells from primary cultures agglutinated much less readily than did cells from WISH populations. Furthermore, the former exhibited a drastic decline in agglutinability as a function of time in suspension culture after trypsinization. Short-term exposure (60 h) of HAM cells in monolayer culture to 5-bromodeoxyuridine (BrdU) elicited heightened agglutinability detectable through 22 days in vitro. Addition of the protease inhibitors n-tosyl-L-lysyl-chloromethyl ketone (TLCK) or p-tosyl-L-arginine-methyl ester (TAME) to the culture medium inhibited proliferation of the WISH line by 40--50% while effecting only a 10-15% inhibition of HAM cells. These results also confirm data with other cell species indicating that high proteolytic activity at the surface of transformed cells may be related to the rapid proliferation rate.", "PMID": 965429}
{"id": "pubmed23n0032_6335", "title": "Pinocytosis and intracellular digestion of 125I-labelled haemoglobin by trophoblastic cells in tissue culture in the presence and absence of serum.", "content": "One aspect of human placental function which has not hitherto been studied is the ability of the placenta to digest proteins intracellularly and use the products of hydrolysis to supply its own and foetall complement of hydrolytic enzymes, including the acid proteases cathepsin C and D. We have used trophoblast cells in monolayer tissue culture as a model for the study of endocytosis and intracellular digestion of 125I-haemoglobin. The normal use of serum in tissue culture medium has shown up differences from the pattern observed with other phagocytic cells such as macrophages, in that serum allows endocytosis but prevents intracellular digestion of 125I-haemoglobin. Replacement of serum by lactalbumin hydrolysate enables both endocytosis and intracellular digestion of 125I-haemoglobin to occur as in other phagocytic cells. Digestion is followed by release into the medium of acid-soluble, lower-molecular-weight compounds. The reasons for this major difference between trophoblast and other cells are discussed in the light of our results and their possible relevance to placental function.", "contents": "Pinocytosis and intracellular digestion of 125I-labelled haemoglobin by trophoblastic cells in tissue culture in the presence and absence of serum. One aspect of human placental function which has not hitherto been studied is the ability of the placenta to digest proteins intracellularly and use the products of hydrolysis to supply its own and foetall complement of hydrolytic enzymes, including the acid proteases cathepsin C and D. We have used trophoblast cells in monolayer tissue culture as a model for the study of endocytosis and intracellular digestion of 125I-haemoglobin. The normal use of serum in tissue culture medium has shown up differences from the pattern observed with other phagocytic cells such as macrophages, in that serum allows endocytosis but prevents intracellular digestion of 125I-haemoglobin. Replacement of serum by lactalbumin hydrolysate enables both endocytosis and intracellular digestion of 125I-haemoglobin to occur as in other phagocytic cells. Digestion is followed by release into the medium of acid-soluble, lower-molecular-weight compounds. The reasons for this major difference between trophoblast and other cells are discussed in the light of our results and their possible relevance to placental function.", "PMID": 965430}
{"id": "pubmed23n0032_6336", "title": "Amino acid metabolism of myeloma cells in culture.", "content": "The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.", "contents": "Amino acid metabolism of myeloma cells in culture. The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.", "PMID": 965431}
{"id": "pubmed23n0032_6337", "title": "Cell wall development of Micrasterias americana, especially in isotonic and hypertonic solutions.", "content": "The cell wall development of Micrasterias americana was investigated by light and electron microscopy. From digestion experiments with pectinase and cellulase, and from fluorescence spectra in Calcofluor and Coriphosphin solution, it was concluded that pectin substances were the main component of the young developing cell wall and that cellulose was synthesized after the daughter hemicell was well developed. In 0-16 M mannitol, wall materials accumulated and were incompletely incorporated into the wall at the region where wall growth would be expected. The plasma membrane was in close contact with the cell wall at the sinus, and this contact was assumed to prevent penetration of wall material at this region, resulting in the accumulation of wall material at regions other than the sinus. The cellulosic wall layer was formed after the production of pectic substances in the 0-16 M mannitol. In 0-3 M mannitol neither a definite wall layer of cellulose nor a pectic wall was produced, presumably due to extensive dilution of the wall materials in the plasmolysed space between the cell wall and the plasma membrane. Under normal circumstances, the shape of the daughter cell is assumed to be determined by the shape of the developed primary wall, which is induced by precocious differentiation of the wall at the sinus.", "contents": "Cell wall development of Micrasterias americana, especially in isotonic and hypertonic solutions. The cell wall development of Micrasterias americana was investigated by light and electron microscopy. From digestion experiments with pectinase and cellulase, and from fluorescence spectra in Calcofluor and Coriphosphin solution, it was concluded that pectin substances were the main component of the young developing cell wall and that cellulose was synthesized after the daughter hemicell was well developed. In 0-16 M mannitol, wall materials accumulated and were incompletely incorporated into the wall at the region where wall growth would be expected. The plasma membrane was in close contact with the cell wall at the sinus, and this contact was assumed to prevent penetration of wall material at this region, resulting in the accumulation of wall material at regions other than the sinus. The cellulosic wall layer was formed after the production of pectic substances in the 0-16 M mannitol. In 0-3 M mannitol neither a definite wall layer of cellulose nor a pectic wall was produced, presumably due to extensive dilution of the wall materials in the plasmolysed space between the cell wall and the plasma membrane. Under normal circumstances, the shape of the daughter cell is assumed to be determined by the shape of the developed primary wall, which is induced by precocious differentiation of the wall at the sinus.", "PMID": 965432}
{"id": "pubmed23n0032_6338", "title": "[Experimental study of anatomo-pathological and physiopathological manifestations of acute cholecystitis].", "content": "From their experimental findings in 120 rabbits, the authors conclude that, at first, cholecystitis is usually an aseptic lesion and infection occurs only secondarily. In a large number of cases the initial physiopathological mechanism is that of inflammation which may be due to mechanical causes such as obstruction of the gall bladder siphon and vasomotor phenomena under autonomic control. The histological lesions and course are comparable to those observed in clinical medicine. The interest of this experimental study is to compare the pathology of acute cholecystitis with pancreatitis and Reilly's syndrome and Gregoire and Couvelaire's theory of visceral apoplexy.", "contents": "[Experimental study of anatomo-pathological and physiopathological manifestations of acute cholecystitis]. From their experimental findings in 120 rabbits, the authors conclude that, at first, cholecystitis is usually an aseptic lesion and infection occurs only secondarily. In a large number of cases the initial physiopathological mechanism is that of inflammation which may be due to mechanical causes such as obstruction of the gall bladder siphon and vasomotor phenomena under autonomic control. The histological lesions and course are comparable to those observed in clinical medicine. The interest of this experimental study is to compare the pathology of acute cholecystitis with pancreatitis and Reilly's syndrome and Gregoire and Couvelaire's theory of visceral apoplexy.", "PMID": 965437}
{"id": "pubmed23n0032_6339", "title": "[Hepatic surgery with vascular exclusion. Experimental bases. Technic and preliminary results].", "content": "Hepatic surgery has a high mortality due to hemorrhage or gas embolism due to tears of the hepatic veins or vena cava, hence the idea of temporary vascular exclusion. Based on clinical, hemodynamic, laboratory and pathological findings, we have designed a catheter which permits one to isolate the retro and suprahepatic segment of the vena cana and obtain hepatic vascular exclusion within 30 minutes. We report here six cases where this technic was used, 3 hepatectomies, 2 liver injuries, and one case of disobstruction of an iliac vein which confirm the use, ease and harmlessness of this method.", "contents": "[Hepatic surgery with vascular exclusion. Experimental bases. Technic and preliminary results]. Hepatic surgery has a high mortality due to hemorrhage or gas embolism due to tears of the hepatic veins or vena cava, hence the idea of temporary vascular exclusion. Based on clinical, hemodynamic, laboratory and pathological findings, we have designed a catheter which permits one to isolate the retro and suprahepatic segment of the vena cana and obtain hepatic vascular exclusion within 30 minutes. We report here six cases where this technic was used, 3 hepatectomies, 2 liver injuries, and one case of disobstruction of an iliac vein which confirm the use, ease and harmlessness of this method.", "PMID": 965438}
{"id": "pubmed23n0032_6340", "title": "[Injuries of the abdominal aorta. General review apropos of a late discovered case].", "content": "The authors present a case of acute ischemia of one lower limb with a an uncommon etiology. The cause of embolism was an incomplete thrombosis of the abdominal aorta; this followed a blunt trauma occured three months before. Some similar cases, reported in the litterature, are resumed. This observation permits the authors to review other cases of rupture and post-traumatic thrombosis of the abdominal aorta.", "contents": "[Injuries of the abdominal aorta. General review apropos of a late discovered case]. The authors present a case of acute ischemia of one lower limb with a an uncommon etiology. The cause of embolism was an incomplete thrombosis of the abdominal aorta; this followed a blunt trauma occured three months before. Some similar cases, reported in the litterature, are resumed. This observation permits the authors to review other cases of rupture and post-traumatic thrombosis of the abdominal aorta.", "PMID": 965439}
{"id": "pubmed23n0032_6341", "title": "[Crohn's disease. Apropos of 72 surgical cases].", "content": "The authors report a series of 72 cases of Crohn's disease, and discuss the clinical and pathological aspects, the surgical treatment and results. A critical study of the operations in view of the requirements of the disease showed that incomplete resection was mainly responsible for the recurrences. In most cases, the word relapse was more suitable to describe the poor results noted. Immediate radical removal gives such good results that these outweigh the functional disadvantages.", "contents": "[Crohn's disease. Apropos of 72 surgical cases]. The authors report a series of 72 cases of Crohn's disease, and discuss the clinical and pathological aspects, the surgical treatment and results. A critical study of the operations in view of the requirements of the disease showed that incomplete resection was mainly responsible for the recurrences. In most cases, the word relapse was more suitable to describe the poor results noted. Immediate radical removal gives such good results that these outweigh the functional disadvantages.", "PMID": 965440}
{"id": "pubmed23n0032_6342", "title": "[Chronic painless pancreatitis. Apropos of 9 cases].", "content": "The authors report 9 cases of painless chronic pancreatitis and review the literature on this apparently rare condition. They emphasise the contrast between absent or minimal pain and the severe pathological lesions. In the light of these cases and pathological specimens, they attempt to interpret the pain during chronic pancreatitis. Pain seems to occur when there is a downhill course.", "contents": "[Chronic painless pancreatitis. Apropos of 9 cases]. The authors report 9 cases of painless chronic pancreatitis and review the literature on this apparently rare condition. They emphasise the contrast between absent or minimal pain and the severe pathological lesions. In the light of these cases and pathological specimens, they attempt to interpret the pain during chronic pancreatitis. Pain seems to occur when there is a downhill course.", "PMID": 965441}
{"id": "pubmed23n0032_6343", "title": "[Thrombo-embolitic complications due to total hip prostheses. Results of preventive treatment using low-dose heparin therapy].", "content": "Our experience of the prevention of thrombosis and embolism by the use of low doses of calcium heparinate in total hip replacement, with individual variations, shows that this method is efficacious, convenient and has a low complication rate both locally and generally.", "contents": "[Thrombo-embolitic complications due to total hip prostheses. Results of preventive treatment using low-dose heparin therapy]. Our experience of the prevention of thrombosis and embolism by the use of low doses of calcium heparinate in total hip replacement, with individual variations, shows that this method is efficacious, convenient and has a low complication rate both locally and generally.", "PMID": 965442}
{"id": "pubmed23n0032_6344", "title": "[Spontaneous rupture of an aneuysm of the abdominal aorta into the inferior vena cava].", "content": "Rupture of an aortic aneurysm into the inferior vena cava is a rare complication; it represents 3 p. 100 of all ruptured aneurysms. The authors report a case with a typical clinical history and 3 main symptoms : lumbar pain, a pulsatile expansile mass, a continuous abdominal murmur giving rise to a thrill. The diagnosis was confirmed before operation by angiography which showed a fistula. A technical detail is emphasised: clamping of the inferior vena cava opening the aneurysm to reduce the risk of embolism into the right side of the heart during operation.", "contents": "[Spontaneous rupture of an aneuysm of the abdominal aorta into the inferior vena cava]. Rupture of an aortic aneurysm into the inferior vena cava is a rare complication; it represents 3 p. 100 of all ruptured aneurysms. The authors report a case with a typical clinical history and 3 main symptoms : lumbar pain, a pulsatile expansile mass, a continuous abdominal murmur giving rise to a thrill. The diagnosis was confirmed before operation by angiography which showed a fistula. A technical detail is emphasised: clamping of the inferior vena cava opening the aneurysm to reduce the risk of embolism into the right side of the heart during operation.", "PMID": 965443}
{"id": "pubmed23n0032_6345", "title": "[Physiopathology of the Zollinger-Ellison syndrome. Present aspects].", "content": "Recent technics, e.g. radio-immunoassay of gastrin (MacGuigan 1968), cellular immuno-fluroescence (Polak and Pearse 1972) have modified our concepts of the physiopathology of the Zollinger-Ellison syndrome hypergastrinemia of pancreatic origin. 1. Pollak and Pearse, (1972) Ganguli, Pollak and Pearse (1974) have shown that apart from the Zollinger-Ellison syndrome of pancreatic origin (ZE type II) there are cases where the hypergastrinemia is due to hyperplasia of the antral \"G\" cells which secrete gastrin (ZE Tye I). Thus is raised the problem of the relationship between common peptic ulcer, \"G\" cell antral hyperplasia and pancreatic hypergastrinemia. 2. There exists, in the anterior part of the intestine (pancreas, duodenum, stomach, ileum) a system of neuro-humoral cells derived embryologically from the neural crest (Weichert 1967, Gorin and Leger 1969). These pluripotent cells may be the origin of digestive endocrine, or carcinoid tumours, islet cell adenomas, or gastrinomas. Thus may be explained among others the multihormonal secretion of some of these tumours and the frequently multiple sites. 3. Gastrin is secreted by the duodenal \"G\" cells (Watson 1974). Their physiopathological role is still unknown.", "contents": "[Physiopathology of the Zollinger-Ellison syndrome. Present aspects]. Recent technics, e.g. radio-immunoassay of gastrin (MacGuigan 1968), cellular immuno-fluroescence (Polak and Pearse 1972) have modified our concepts of the physiopathology of the Zollinger-Ellison syndrome hypergastrinemia of pancreatic origin. 1. Pollak and Pearse, (1972) Ganguli, Pollak and Pearse (1974) have shown that apart from the Zollinger-Ellison syndrome of pancreatic origin (ZE type II) there are cases where the hypergastrinemia is due to hyperplasia of the antral \"G\" cells which secrete gastrin (ZE Tye I). Thus is raised the problem of the relationship between common peptic ulcer, \"G\" cell antral hyperplasia and pancreatic hypergastrinemia. 2. There exists, in the anterior part of the intestine (pancreas, duodenum, stomach, ileum) a system of neuro-humoral cells derived embryologically from the neural crest (Weichert 1967, Gorin and Leger 1969). These pluripotent cells may be the origin of digestive endocrine, or carcinoid tumours, islet cell adenomas, or gastrinomas. Thus may be explained among others the multihormonal secretion of some of these tumours and the frequently multiple sites. 3. Gastrin is secreted by the duodenal \"G\" cells (Watson 1974). Their physiopathological role is still unknown.", "PMID": 965444}
{"id": "pubmed23n0032_6346", "title": "A method for the estimation of free and esterified cholesterol involving thin-layer chromatography.", "content": "A method for the estimation of both free and bound cholesterol in serum and plasma involving thin-layer chromatography has been developed. The serum or plasma is applied as a band directly on the plate. Extraction of lipid material from this band and the chromatographic separation of the above components are carried out on the same plate. The components are extracted from the adsorbent directly with acetic acid and measured colorimetrically using iron(III) chloride-sulphuric acid reagent.", "contents": "A method for the estimation of free and esterified cholesterol involving thin-layer chromatography. A method for the estimation of both free and bound cholesterol in serum and plasma involving thin-layer chromatography has been developed. The serum or plasma is applied as a band directly on the plate. Extraction of lipid material from this band and the chromatographic separation of the above components are carried out on the same plate. The components are extracted from the adsorbent directly with acetic acid and measured colorimetrically using iron(III) chloride-sulphuric acid reagent.", "PMID": 965455}
{"id": "pubmed23n0032_6347", "title": "Identification of antibiotic residues in milk by thin-layer chromatography.", "content": "A scheme has been devised that makes it possible to separate and identify, by means of thin-layer chromatography, the 14 different antibiotic resudues in milk which are, besides penicillin, the most widely used in mastitis control: cloxacillin, dihydrostreptomycin, tetracycline, oxytetracycline, chlortetracycline, chloramphenicol, neomycin, novobiocin, bacitracin, erythromycin, oleandomycin, ampicillin, streptomycin and oxacillin. The limits of detectability of the antibiotics studied vary between 0.1 and 3 mug/ml, with the exception of neomycin the minimum detectable concentration of which is 15 mug/ml.", "contents": "Identification of antibiotic residues in milk by thin-layer chromatography. A scheme has been devised that makes it possible to separate and identify, by means of thin-layer chromatography, the 14 different antibiotic resudues in milk which are, besides penicillin, the most widely used in mastitis control: cloxacillin, dihydrostreptomycin, tetracycline, oxytetracycline, chlortetracycline, chloramphenicol, neomycin, novobiocin, bacitracin, erythromycin, oleandomycin, ampicillin, streptomycin and oxacillin. The limits of detectability of the antibiotics studied vary between 0.1 and 3 mug/ml, with the exception of neomycin the minimum detectable concentration of which is 15 mug/ml.", "PMID": 965456}
{"id": "pubmed23n0032_6348", "title": "An improved technique for the gas-liquid chromatographic separation of the N-trifluoroacetyl n-butyl derivatives of amino acids.", "content": "A complete separation of the twenty protein amino acids and two internal standards in 35 min has been made possible by analyzing their N-trifluoroacetyl n-butyl (TAB) derivatives simultaneously on an ethylene glycol adipate (EGA) column and on an OV-17 column using the same temperature programme. The detector signal from the EGA column is recorded up to the point just after the elution of TAB-aspartic acid; thereafter, the signal from the effluent of the OV-17 column is recorded. The TAB derivative of norleucine is poorly resolved from TAB-leucine and TAB-proline. Therefore, alpha-aminocaprylic acid has been used as the internal standard instead of noreleucine. TAB-alpha-aminocaprylic acid appears as a separate peak between TAB-serine and TAB-cysteine.", "contents": "An improved technique for the gas-liquid chromatographic separation of the N-trifluoroacetyl n-butyl derivatives of amino acids. A complete separation of the twenty protein amino acids and two internal standards in 35 min has been made possible by analyzing their N-trifluoroacetyl n-butyl (TAB) derivatives simultaneously on an ethylene glycol adipate (EGA) column and on an OV-17 column using the same temperature programme. The detector signal from the EGA column is recorded up to the point just after the elution of TAB-aspartic acid; thereafter, the signal from the effluent of the OV-17 column is recorded. The TAB derivative of norleucine is poorly resolved from TAB-leucine and TAB-proline. Therefore, alpha-aminocaprylic acid has been used as the internal standard instead of noreleucine. TAB-alpha-aminocaprylic acid appears as a separate peak between TAB-serine and TAB-cysteine.", "PMID": 965460}
{"id": "pubmed23n0032_6349", "title": "Selective detection of N-nitrosamines by gas chromatography using a modified microelectrolytic conductivity detector in the pyrolytic mode.", "content": "The pyrolysis of nitrosamines in a modified Hall microelectrolytic conductivity system provides the basis for a highly selective and sensitive determination method. Under the conditions specified, the response of this detector system to nitrosamines is at least 10(7) times greater than that for n-hexane. The influence of several operational parameters on the response of the detector to nitrosamines was studied. For open-chain nitrosamines, the detection limit is 50 pg and the response is linear up to at least 200 ng.", "contents": "Selective detection of N-nitrosamines by gas chromatography using a modified microelectrolytic conductivity detector in the pyrolytic mode. The pyrolysis of nitrosamines in a modified Hall microelectrolytic conductivity system provides the basis for a highly selective and sensitive determination method. Under the conditions specified, the response of this detector system to nitrosamines is at least 10(7) times greater than that for n-hexane. The influence of several operational parameters on the response of the detector to nitrosamines was studied. For open-chain nitrosamines, the detection limit is 50 pg and the response is linear up to at least 200 ng.", "PMID": 965461}
{"id": "pubmed23n0032_6350", "title": "Determination of diamines and polyamines in tissues by high-pressure liquid chromatography.", "content": "The 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) derivatives of 1,2-diaminoethane, 1,3-diaminopropane, 1,4-diaminobutane (putrescine), spermidine and spermine have been separated by high-pressure liquid chromatography on a Micropak CN-10 column using a programmed solvent gradient elution. The column eluate is monitored by a fluorescence detector. This method has been used to determine the levles of putrescine, spermidine and spermine in various tissues of rats and in L 1210 leukemic cells of mice grown in culture. The technique is sufficiently sensitive to detect ca. 40pmoles of putrescine and ca. 20 pmoles of spermidine and spermine, is quite specific and can be performed rapidly.", "contents": "Determination of diamines and polyamines in tissues by high-pressure liquid chromatography. The 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) derivatives of 1,2-diaminoethane, 1,3-diaminopropane, 1,4-diaminobutane (putrescine), spermidine and spermine have been separated by high-pressure liquid chromatography on a Micropak CN-10 column using a programmed solvent gradient elution. The column eluate is monitored by a fluorescence detector. This method has been used to determine the levles of putrescine, spermidine and spermine in various tissues of rats and in L 1210 leukemic cells of mice grown in culture. The technique is sufficiently sensitive to detect ca. 40pmoles of putrescine and ca. 20 pmoles of spermidine and spermine, is quite specific and can be performed rapidly.", "PMID": 965462}
{"id": "pubmed23n0032_6351", "title": "Comparative biochemistry and pharmacology of salivary gland secretions. III. Chromatographic isolation of a myocardial depressor protein (MDP) from the venom of Crotalus atrox.", "content": "A protein has been isolated from the venom of the western diamondback rattlesnake (Crotalus atrox) which induces acute myocardial depression when administered to experimental animals. Purification was achieved by gel filtration on Sephadex G-100, DEAE- and CM-cellulose ion-exchange chromatography, ultra-filtration, and adsorption chromatography on hydroxyapatite. Amino acid analysis of the highly purified protein indicated N-terminal isoleucine and C-terminal tyrosine residues, and the absence of free sulfhydryl groups. Rabbits were immunized against the myocardial depressor protein (MDP) and a highly specific antiserum prepared which made it possible to study other snake venoms for the presence or absence of MDP. All of the North American Crotalid species of snakes contain MDP in varying degrees of concentration, but none of the Asiatic snake venoms tested reacted with the antiserum to the myocardial depressor protein. Intravenous administration of MDP to experimental animals (dogs, cats) produces an immediate and profound decrease in the cardiac output, the left ventricular systolic and mean pressures, the velocity of shortening of the contractile element, the systemic arterial pressure and an elevation in the left ventricular end-diastolic and pulmonary wedge pressures. These hemodynamic changes indicate that MDP administration induces an acute myocardial failure which is does dependent. The potential use of this protein for the reproducible causation of left ventricular failure, obviating the need for the more commonly used surgical ligation of the coronary arteries, warrants a full investigation into its structure, active site and its mechanism of action on the myocardial cell.", "contents": "Comparative biochemistry and pharmacology of salivary gland secretions. III. Chromatographic isolation of a myocardial depressor protein (MDP) from the venom of Crotalus atrox. A protein has been isolated from the venom of the western diamondback rattlesnake (Crotalus atrox) which induces acute myocardial depression when administered to experimental animals. Purification was achieved by gel filtration on Sephadex G-100, DEAE- and CM-cellulose ion-exchange chromatography, ultra-filtration, and adsorption chromatography on hydroxyapatite. Amino acid analysis of the highly purified protein indicated N-terminal isoleucine and C-terminal tyrosine residues, and the absence of free sulfhydryl groups. Rabbits were immunized against the myocardial depressor protein (MDP) and a highly specific antiserum prepared which made it possible to study other snake venoms for the presence or absence of MDP. All of the North American Crotalid species of snakes contain MDP in varying degrees of concentration, but none of the Asiatic snake venoms tested reacted with the antiserum to the myocardial depressor protein. Intravenous administration of MDP to experimental animals (dogs, cats) produces an immediate and profound decrease in the cardiac output, the left ventricular systolic and mean pressures, the velocity of shortening of the contractile element, the systemic arterial pressure and an elevation in the left ventricular end-diastolic and pulmonary wedge pressures. These hemodynamic changes indicate that MDP administration induces an acute myocardial failure which is does dependent. The potential use of this protein for the reproducible causation of left ventricular failure, obviating the need for the more commonly used surgical ligation of the coronary arteries, warrants a full investigation into its structure, active site and its mechanism of action on the myocardial cell.", "PMID": 965463}
{"id": "pubmed23n0032_6352", "title": "Gas-liquid chromatography of amino acids. Determination of cystine and cysteine as N-acetyl, n-propyl S-carboxymethylcysteinate.", "content": "A procedure is described for the derivatisation of S-carboxymethylcysteine yielding the corresponding N-acetyl, n-propyl S-carboxymethylcysteinate which is separated by gas chromatography form similar derivatives of other protein amino acids. Its quantitative determination provides a useful extension to a method for analysis of amino acids by gas chromatography and offers potential as a means of obviating errors in cystine (and/or cysteine) analysis arising from acid hydrolysis of proteins.", "contents": "Gas-liquid chromatography of amino acids. Determination of cystine and cysteine as N-acetyl, n-propyl S-carboxymethylcysteinate. A procedure is described for the derivatisation of S-carboxymethylcysteine yielding the corresponding N-acetyl, n-propyl S-carboxymethylcysteinate which is separated by gas chromatography form similar derivatives of other protein amino acids. Its quantitative determination provides a useful extension to a method for analysis of amino acids by gas chromatography and offers potential as a means of obviating errors in cystine (and/or cysteine) analysis arising from acid hydrolysis of proteins.", "PMID": 965464}
{"id": "pubmed23n0032_6353", "title": "Separation of permethylated isomeric glucuronides by gas chromatography and analysis of the mass spectra.", "content": "Glucuronic acid conjugates of several foreign compounds (1- and 2-naphthols, 2-, 3-, and 4-hydroxybiphenyls, m- and p-hydroxyphenylphenylhydantoins) were produced in the isolated perfused rat liver and identified in the bile as the permethyl derivatives. Permethylated glucuronide isomers were easily separated by gas chromatography on SE-30 and OV-17 columns. The mass spectra of permethylated glucuronide isomers were very similar. Gas chromatography proved to be most useful for the separation and identification of isomeric glucuronides.", "contents": "Separation of permethylated isomeric glucuronides by gas chromatography and analysis of the mass spectra. Glucuronic acid conjugates of several foreign compounds (1- and 2-naphthols, 2-, 3-, and 4-hydroxybiphenyls, m- and p-hydroxyphenylphenylhydantoins) were produced in the isolated perfused rat liver and identified in the bile as the permethyl derivatives. Permethylated glucuronide isomers were easily separated by gas chromatography on SE-30 and OV-17 columns. The mass spectra of permethylated glucuronide isomers were very similar. Gas chromatography proved to be most useful for the separation and identification of isomeric glucuronides.", "PMID": 965465}
{"id": "pubmed23n0032_6354", "title": "Gas chromatographic determination of acenocoumarin in human plasma.", "content": "A method based on solvent extraction, formation of a fluorinated derivative and quantitation by gas-liquid chromatography with electron-capture detection has been developed for the determination of acenocoumarin in plasma. The specificity and sensitivity of the procedure appear to be satisfactory for drug level measurements in human plasma. Its relative simplicity permits its use in routine analysis.", "contents": "Gas chromatographic determination of acenocoumarin in human plasma. A method based on solvent extraction, formation of a fluorinated derivative and quantitation by gas-liquid chromatography with electron-capture detection has been developed for the determination of acenocoumarin in plasma. The specificity and sensitivity of the procedure appear to be satisfactory for drug level measurements in human plasma. Its relative simplicity permits its use in routine analysis.", "PMID": 965466}
{"id": "pubmed23n0032_6355", "title": "[High-performance liquid chromatography of penimocycline (author's transl)].", "content": "Penimocycline is an antibiotic obtained by Mannich reaction between tetracycline and ampicilline. Separation of penimocycline from tetracyclines and other impurities has been studied by high-performance liquid chromatography. The most effective method is liquid-liquid partition on a Micropak CH column (non-polar hydrocarbon bonded on porous silica microparticles) and gradient elution with water-methanol, 0.02 M phosphate buffer (pH 7.6) and 1 mM EDTA. Some results on hydrolysis of penimocycline are given.", "contents": "[High-performance liquid chromatography of penimocycline (author's transl)]. Penimocycline is an antibiotic obtained by Mannich reaction between tetracycline and ampicilline. Separation of penimocycline from tetracyclines and other impurities has been studied by high-performance liquid chromatography. The most effective method is liquid-liquid partition on a Micropak CH column (non-polar hydrocarbon bonded on porous silica microparticles) and gradient elution with water-methanol, 0.02 M phosphate buffer (pH 7.6) and 1 mM EDTA. Some results on hydrolysis of penimocycline are given.", "PMID": 965467}
{"id": "pubmed23n0032_6356", "title": "Determination of nanogram amounts of aromatic compounds by spectrophotometry on thin-layer chromatograms.", "content": "A highly sensitive method is described for the simultaneous determination of codeine and chlorpheniramine in plasma. Thin-layer chromatography is used for the separation of the drugs. The spots are then rendered visible by nitration of the substances on the thin-layer plate. Codeine can be quantified by direct measurement of the resulting fluorescence. After reduction, the aromatic amines are diazotized and coupled with N-(1-naphthyl)ethylenediamine on the thin-layer plate. For codeine the fluorimetric measurement is more reliable than the colorimetric determination. The sensitivity limits are 8 ng/ml of plasma for codeine phosphate and 1-2 ng/ml of plasma for chlorpheniramine maleate. This procedure is also applicable to other aromatic compounds which can be nitrated by the described method. The method has been applied to compare a determination of the plasma levels of codeine and chlorpheniramine after administration in normal capsules and retard tablets.", "contents": "Determination of nanogram amounts of aromatic compounds by spectrophotometry on thin-layer chromatograms. A highly sensitive method is described for the simultaneous determination of codeine and chlorpheniramine in plasma. Thin-layer chromatography is used for the separation of the drugs. The spots are then rendered visible by nitration of the substances on the thin-layer plate. Codeine can be quantified by direct measurement of the resulting fluorescence. After reduction, the aromatic amines are diazotized and coupled with N-(1-naphthyl)ethylenediamine on the thin-layer plate. For codeine the fluorimetric measurement is more reliable than the colorimetric determination. The sensitivity limits are 8 ng/ml of plasma for codeine phosphate and 1-2 ng/ml of plasma for chlorpheniramine maleate. This procedure is also applicable to other aromatic compounds which can be nitrated by the described method. The method has been applied to compare a determination of the plasma levels of codeine and chlorpheniramine after administration in normal capsules and retard tablets.", "PMID": 965468}
{"id": "pubmed23n0032_6357", "title": "Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.", "content": "A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.", "contents": "Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies. A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.", "PMID": 965475}
{"id": "pubmed23n0032_6358", "title": "Enhancement by sodium chloride of the selectivity of thiosulfate citrate bile salts sucrose agar for isolating Vibrio cholerae biotype El Tor.", "content": "In this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of NaCl to thiosulfate citrate bile salts sucrose (TCBS) agar. The effect of adding salt to TCBS agar varied greatly among brands of TCBS agar and between lots of the same brand. The addition of salt at concentrations as high as 1.5% (2.5% total NaCl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical Vibrio cholerae but did not compromise the use of TCBS agar for isolation of V. cholerae biotype El Tor.", "contents": "Enhancement by sodium chloride of the selectivity of thiosulfate citrate bile salts sucrose agar for isolating Vibrio cholerae biotype El Tor. In this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of NaCl to thiosulfate citrate bile salts sucrose (TCBS) agar. The effect of adding salt to TCBS agar varied greatly among brands of TCBS agar and between lots of the same brand. The addition of salt at concentrations as high as 1.5% (2.5% total NaCl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical Vibrio cholerae but did not compromise the use of TCBS agar for isolation of V. cholerae biotype El Tor.", "PMID": 965476}
{"id": "pubmed23n0032_6359", "title": "Yersinia enterocolitica isolates from humans in California, 1968-1975.", "content": "This paper reports on the serological and biochemical characteristics of 24 human isolates of Yersinia enterocolitica submitted to the California Department of Health from 1968 through 1975. Nine different serotypes were represented. The majority of strains were serotype O:8 (six strains) and serotype O:5 (five strains). Sources of the isolates included feces (12 cases), blood (3), sputum or throat (3), bile or bowel drainage (2), wounds (2), breast abscess (1), and skin abscess (1). Clinical histories indicated a number of different syndromes. Underlying medical conditions existed in 13 cases. Results of selected biochemical tests and antimicrobial susceptibility tests on the strains indicated grouping compatible with the O serotypes of the organisms.", "contents": "Yersinia enterocolitica isolates from humans in California, 1968-1975. This paper reports on the serological and biochemical characteristics of 24 human isolates of Yersinia enterocolitica submitted to the California Department of Health from 1968 through 1975. Nine different serotypes were represented. The majority of strains were serotype O:8 (six strains) and serotype O:5 (five strains). Sources of the isolates included feces (12 cases), blood (3), sputum or throat (3), bile or bowel drainage (2), wounds (2), breast abscess (1), and skin abscess (1). Clinical histories indicated a number of different syndromes. Underlying medical conditions existed in 13 cases. Results of selected biochemical tests and antimicrobial susceptibility tests on the strains indicated grouping compatible with the O serotypes of the organisms.", "PMID": 965477}
{"id": "pubmed23n0032_6360", "title": "Evaluation of a new system for the rapid identification of clinically important yeasts.", "content": "The rapid system developed by Huppert et al. (1975) for the identification of yeasts based on assimilation and fermentation patterns and on germ tube and pseudohyphal production was evaluated in a comparative study with conventional procedures. The 95 test cultures were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The study revealed that approximately 94% of the isolates were correctly identified by the rapid method in comparison with the standard method. With the rapid method identification was accomplished in 72h, and with the conventional procedures identification was completed in 2 weeks. Although it was difficult with some isolates to obtain definitive speciation by the rapid method, this method promises to be especially useful in clinical laboratories for the identification of yeasts of medical importance. Modifications were made in the procedure of Huppert et al. (1975) to improve the reading of reactions. Commercial media and a disk dispenser to make the method more useful were also investigated.", "contents": "Evaluation of a new system for the rapid identification of clinically important yeasts. The rapid system developed by Huppert et al. (1975) for the identification of yeasts based on assimilation and fermentation patterns and on germ tube and pseudohyphal production was evaluated in a comparative study with conventional procedures. The 95 test cultures were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The study revealed that approximately 94% of the isolates were correctly identified by the rapid method in comparison with the standard method. With the rapid method identification was accomplished in 72h, and with the conventional procedures identification was completed in 2 weeks. Although it was difficult with some isolates to obtain definitive speciation by the rapid method, this method promises to be especially useful in clinical laboratories for the identification of yeasts of medical importance. Modifications were made in the procedure of Huppert et al. (1975) to improve the reading of reactions. Commercial media and a disk dispenser to make the method more useful were also investigated.", "PMID": 965478}
{"id": "pubmed23n0032_6361", "title": "Isolation of Vibrio parahaemolyticus from fecal specimens on mannitol salt agar.", "content": "Unless laboratories use an inhibitory medium, Vibrio parahaemolyticus will be unrecognizable in fecal specimens. The use of a medium exclusively for vibrio isolation, such as thiosulfate-citrate-bile salts-sucrose agar (TCBS), however, may not be considered economically justified in the United States. The isolation and recognition of V. parahaemolyticus is reported on mannitol salt agar (MS), a medium which is used for fecal specimens here. Eight Kanagawa-positive and two of three Kanagawa-negative strains of V. parahaemolyticus grew as well on MS as on TCBS and better than on a representative enteric medium, Hektoen enteric agar (HE). Twenty-two fecal specimens from 16 noninfected individuals were inoculated with known quantities of V. parahaemolyticus, and recovery of these vibrios was assessed on TCBS, MS, and HE. Recovery of vibrios from MS and TCBS was similar when inoculum size was 10(3) colony-forming units/ml or greater. Recovery of vibrios from mixed culture was distinctly lower on HE. The colonial morphology of V. parahaemolyticus and several other bacteria on MS is illustrated.", "contents": "Isolation of Vibrio parahaemolyticus from fecal specimens on mannitol salt agar. Unless laboratories use an inhibitory medium, Vibrio parahaemolyticus will be unrecognizable in fecal specimens. The use of a medium exclusively for vibrio isolation, such as thiosulfate-citrate-bile salts-sucrose agar (TCBS), however, may not be considered economically justified in the United States. The isolation and recognition of V. parahaemolyticus is reported on mannitol salt agar (MS), a medium which is used for fecal specimens here. Eight Kanagawa-positive and two of three Kanagawa-negative strains of V. parahaemolyticus grew as well on MS as on TCBS and better than on a representative enteric medium, Hektoen enteric agar (HE). Twenty-two fecal specimens from 16 noninfected individuals were inoculated with known quantities of V. parahaemolyticus, and recovery of these vibrios was assessed on TCBS, MS, and HE. Recovery of vibrios from MS and TCBS was similar when inoculum size was 10(3) colony-forming units/ml or greater. Recovery of vibrios from mixed culture was distinctly lower on HE. The colonial morphology of V. parahaemolyticus and several other bacteria on MS is illustrated.", "PMID": 965479}
{"id": "pubmed23n0032_6362", "title": "SXT and Taxo A disks for presumptive identification of group A and B streptococci in throat cultures.", "content": "A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B.", "contents": "SXT and Taxo A disks for presumptive identification of group A and B streptococci in throat cultures. A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B.", "PMID": 965480}
{"id": "pubmed23n0032_6363", "title": "Phage typing set for E1, E2, E3, and E4 salmonellae.", "content": "Eighteen common serotypes representative of group E(1), E(2), E(3), and E(4)Salmonella were characterized using a single set of phages.", "contents": "Phage typing set for E1, E2, E3, and E4 salmonellae. Eighteen common serotypes representative of group E(1), E(2), E(3), and E(4)Salmonella were characterized using a single set of phages.", "PMID": 965481}
{"id": "pubmed23n0032_6364", "title": "Compositional mapping of cholesteryl ester droplets in the fatty streaks of human aorta.", "content": "Frozen sections prepared from human aortic tissue containing fatty streak lesions were examined on a thermally controlled stage with a polarizing light microscope. Distinct birefringent droplets, 0.5-5 mum in diameter, were observed, many apparently aggregated into clusters. The clusters were about 20 X 20 mum in diameter (the approximate size of foam cells). Upon being heated, each smectic droplet exhibited a sudden change of birefringence, indicating a change of state. The transition temperatures were compared to assess compositional distributions in the tissue. We found that for 52% of the clusters the standard deviation of the cluster's droplet melting point distribution was less than half that observed in the surrounding microscopic field. If clusters were intracellular lipid inclusions, this observation indicates that the lipid composition within a foam cell is more homogeneous than that of the overall field. However, using statistical methods, we compared droplet melting populations from cluster to cluster and found significant heterogeneity. The observations can be interpreted to suggest that many foam cells modify the cholesteryl ester fatty acid composition of their accumulations be selective uptake, temporal sampling, or chemical reaction. Furthermore, the intercellular heterogeneity suggests that different cells in the lesion may have different metabolic and transport enzyme affinities or be in different states.", "contents": "Compositional mapping of cholesteryl ester droplets in the fatty streaks of human aorta. Frozen sections prepared from human aortic tissue containing fatty streak lesions were examined on a thermally controlled stage with a polarizing light microscope. Distinct birefringent droplets, 0.5-5 mum in diameter, were observed, many apparently aggregated into clusters. The clusters were about 20 X 20 mum in diameter (the approximate size of foam cells). Upon being heated, each smectic droplet exhibited a sudden change of birefringence, indicating a change of state. The transition temperatures were compared to assess compositional distributions in the tissue. We found that for 52% of the clusters the standard deviation of the cluster's droplet melting point distribution was less than half that observed in the surrounding microscopic field. If clusters were intracellular lipid inclusions, this observation indicates that the lipid composition within a foam cell is more homogeneous than that of the overall field. However, using statistical methods, we compared droplet melting populations from cluster to cluster and found significant heterogeneity. The observations can be interpreted to suggest that many foam cells modify the cholesteryl ester fatty acid composition of their accumulations be selective uptake, temporal sampling, or chemical reaction. Furthermore, the intercellular heterogeneity suggests that different cells in the lesion may have different metabolic and transport enzyme affinities or be in different states.", "PMID": 965482}
{"id": "pubmed23n0032_6365", "title": "Direct measurement of papillary collecting duct sodium transport in the rat. Evidence for heterogeneity of nephron function during Ringer loading.", "content": "It has been suggested that collecting duct sodium transport was inhibited by extracellular volume expansion. To directly evaluate this possibility, micropuncture of the papillary collecting duct of young rats was performed during hydropenia and Ringer loading. The possibility of heterogeneity of nephron function was evaluated during Ringer and hyperoncotic albumin loading by comparing the delivery of sodium to the end of the distal tubule of superficial nephrons with papillary base delivery. During hydropenia (n = 14), sodium delivery to the base averaged 0.95% of the filtered sodium load and reabsorption along the collecting duct was noted from base to tip in each collection pair averaging 0.80% of the filtered load. During Ringer loading, sodium delivery to the base was markedly greater than in hydropenia, 11.8 vs. 0.95% of the filtered load (P less than 0.001). Yet, sodium reabsorption was also much greater, 6 vs. 0.8% (P less than 0.001). In 13 paired collections, during Ringer loading, sodium delivery to the papillary base, 12.2% of the filtered load, was consistently greater than late distal tubular delivery from superficial nephrons. 8% (P less than 0.005). In contrast, reabsorption of sodium from late distal tubule to papillary base was found during albumin infusion, 6.2 vs. 3.1% (P less than 0.001). Therefore, these studies demonstrate that: (a) the delivery of sodium to and reabsorption along the papillary collecting duct were markedly greater during Ringer loading than in hydropenia; (b) the amount of sodium delivered to the papillary base was greater than the delivery to the end of the distal tubule of superficial nephrons during Ringer loading, suggesting that deeper nephrons deliver more sodium to the collecting duct in this setting; and (c) the difference in sodium excretion between Ringer loading and hyperoncotic albumin infusion is due to events occurring between the late distal tubule of superficial nephrons and the base of the papillary collecting duct.", "contents": "Direct measurement of papillary collecting duct sodium transport in the rat. Evidence for heterogeneity of nephron function during Ringer loading. It has been suggested that collecting duct sodium transport was inhibited by extracellular volume expansion. To directly evaluate this possibility, micropuncture of the papillary collecting duct of young rats was performed during hydropenia and Ringer loading. The possibility of heterogeneity of nephron function was evaluated during Ringer and hyperoncotic albumin loading by comparing the delivery of sodium to the end of the distal tubule of superficial nephrons with papillary base delivery. During hydropenia (n = 14), sodium delivery to the base averaged 0.95% of the filtered sodium load and reabsorption along the collecting duct was noted from base to tip in each collection pair averaging 0.80% of the filtered load. During Ringer loading, sodium delivery to the base was markedly greater than in hydropenia, 11.8 vs. 0.95% of the filtered load (P less than 0.001). Yet, sodium reabsorption was also much greater, 6 vs. 0.8% (P less than 0.001). In 13 paired collections, during Ringer loading, sodium delivery to the papillary base, 12.2% of the filtered load, was consistently greater than late distal tubular delivery from superficial nephrons. 8% (P less than 0.005). In contrast, reabsorption of sodium from late distal tubule to papillary base was found during albumin infusion, 6.2 vs. 3.1% (P less than 0.001). Therefore, these studies demonstrate that: (a) the delivery of sodium to and reabsorption along the papillary collecting duct were markedly greater during Ringer loading than in hydropenia; (b) the amount of sodium delivered to the papillary base was greater than the delivery to the end of the distal tubule of superficial nephrons during Ringer loading, suggesting that deeper nephrons deliver more sodium to the collecting duct in this setting; and (c) the difference in sodium excretion between Ringer loading and hyperoncotic albumin infusion is due to events occurring between the late distal tubule of superficial nephrons and the base of the papillary collecting duct.", "PMID": 965483}
{"id": "pubmed23n0032_6366", "title": "Further characterization of NADPH oxidase activity of human polymorphonuclear leukocytes.", "content": "Mn2+ was shown to catalyze a nonenzymatic oxidation of NADPH in the presence of superoxide anion by means of an isotopic assay for measurement of the oxidation of NADPH to NADP+. Human polymorphonuclear leukocyte granule NADPH oxidase activity was evaluated in the absence of Mn2+ and was found to be higher in granules from phagocytizing cells than in granules from resting cells. The drug phorbol myristate acetate, which affects the oxidative metabolism of the neutrophil like phagocytosis, was found to activate granule NADPH oxidase activity. Superoxide dismutase was shown to inhibit NADPH oxidase activity both in the presence and absence of added Mn2+. The NADPH oxidase reaction in the absence of Mn2+ was optimal at pH 5.5, and was more linear with increasing time and protein concentration than in the presence of Mn2+. No activity was measurable in granules isolated from resting cells until the level of NADPH added was above 0.25 mM. Activity was present in granules isolated from cells challenged with opsonized zymosan, even at 0.05 mM NADPH, and was higher than the activity found in granule fractions from resting cells at all levels of NADPH tested. The addition of as little as 0.1 muM NADH to the reaction mixture was found to inhibit granular NADPH oxidase activity, indicating a possible regulatory role for NADH. These results suggest that NADPH oxidase may be the enzyme that initiates the metabolic events accompanying phagocytosis.", "contents": "Further characterization of NADPH oxidase activity of human polymorphonuclear leukocytes. Mn2+ was shown to catalyze a nonenzymatic oxidation of NADPH in the presence of superoxide anion by means of an isotopic assay for measurement of the oxidation of NADPH to NADP+. Human polymorphonuclear leukocyte granule NADPH oxidase activity was evaluated in the absence of Mn2+ and was found to be higher in granules from phagocytizing cells than in granules from resting cells. The drug phorbol myristate acetate, which affects the oxidative metabolism of the neutrophil like phagocytosis, was found to activate granule NADPH oxidase activity. Superoxide dismutase was shown to inhibit NADPH oxidase activity both in the presence and absence of added Mn2+. The NADPH oxidase reaction in the absence of Mn2+ was optimal at pH 5.5, and was more linear with increasing time and protein concentration than in the presence of Mn2+. No activity was measurable in granules isolated from resting cells until the level of NADPH added was above 0.25 mM. Activity was present in granules isolated from cells challenged with opsonized zymosan, even at 0.05 mM NADPH, and was higher than the activity found in granule fractions from resting cells at all levels of NADPH tested. The addition of as little as 0.1 muM NADH to the reaction mixture was found to inhibit granular NADPH oxidase activity, indicating a possible regulatory role for NADH. These results suggest that NADPH oxidase may be the enzyme that initiates the metabolic events accompanying phagocytosis.", "PMID": 965484}
{"id": "pubmed23n0032_6367", "title": "Selective uptake of intact parathyroid hormone by the liver: differences between hepatic and renal uptake.", "content": "Hepatic and renal extraction of immunoreactive parathyroid hormone (i-PTH) was studied in awake dogs with explanted kidneys and chronic indwelling hepatic vein catheters. After a single injection of bovine PTH 1-84 (b-PTH 1-84), hepatic arteriovenous (A-V) differences for immunoreactive PTH (i-PTH) was 39% at 2 min after injection but decreased to 0% by 25 min, despite high levels of i-PTH in the arterial circulation. Gel filtration of arterila and hepatic venous samples obtained when hepatic A-V differences for i-PTH were demonstrable revealed hepatic uptake of the intact hormone and addition of a smaller COOH-terminal fragment, eluting just after the intact hormone, to the hepatic venous blood. Gel filtration of samples obtained 20-30 min after injection of b-PTH was demonstrable) revealed no detectable intact hormone in the circulation. Levels of COOH-terminal fragments of the hormone at the time were identical in arterial and hepatic venous samples. In additional experiemtns no hepatic A-V difference was observed after the injection of the synthetic bovine PTH 1-34 (syn b-PTH 1-34). By comparison there was a demonstrable A-V difference of 20% across the kidney for both intact PTH and COOH-terminal fragments that persisted until i-PTH disappeared from the circulation. The kidney also demonstrated an A-V difference of 22% after injection of syn b-PTH 1-34. These studies demonstrate selective extraction of intact PTH but not of its fragments by the liver. The kidney, on the other hand, extracted the intact hormone and both COOH and NH2 terminal fragments. The studies demonstrate that the kidney was the only organ of those examined that detectably removed the fragments of PTH from the circulation.", "contents": "Selective uptake of intact parathyroid hormone by the liver: differences between hepatic and renal uptake. Hepatic and renal extraction of immunoreactive parathyroid hormone (i-PTH) was studied in awake dogs with explanted kidneys and chronic indwelling hepatic vein catheters. After a single injection of bovine PTH 1-84 (b-PTH 1-84), hepatic arteriovenous (A-V) differences for immunoreactive PTH (i-PTH) was 39% at 2 min after injection but decreased to 0% by 25 min, despite high levels of i-PTH in the arterial circulation. Gel filtration of arterila and hepatic venous samples obtained when hepatic A-V differences for i-PTH were demonstrable revealed hepatic uptake of the intact hormone and addition of a smaller COOH-terminal fragment, eluting just after the intact hormone, to the hepatic venous blood. Gel filtration of samples obtained 20-30 min after injection of b-PTH was demonstrable) revealed no detectable intact hormone in the circulation. Levels of COOH-terminal fragments of the hormone at the time were identical in arterial and hepatic venous samples. In additional experiemtns no hepatic A-V difference was observed after the injection of the synthetic bovine PTH 1-34 (syn b-PTH 1-34). By comparison there was a demonstrable A-V difference of 20% across the kidney for both intact PTH and COOH-terminal fragments that persisted until i-PTH disappeared from the circulation. The kidney also demonstrated an A-V difference of 22% after injection of syn b-PTH 1-34. These studies demonstrate selective extraction of intact PTH but not of its fragments by the liver. The kidney, on the other hand, extracted the intact hormone and both COOH and NH2 terminal fragments. The studies demonstrate that the kidney was the only organ of those examined that detectably removed the fragments of PTH from the circulation.", "PMID": 965485}
{"id": "pubmed23n0032_6368", "title": "The origin of the chemiluminescence of phagocytosing granulocytes.", "content": "Granulocytes engaged in the phagocytosis of opsonized zymosan emit light by a process that is inhibited by superoxide dismutase and catalase. In the present report is is shown that light emission is the result of reactions between certain unspecified constituents of the ingested particles and some or all of the oxidizing agents (H2O2, O2),and possibly the hydroxyl radical and singlet oxygen) produced by the activated cells. This conclusion is based on a study of light emission by both activated cells ans artificial O2 generating system containing xanthine oxidase and purine. With these two systems light production required the presence of both zymosan and oxidizing agent, suggesting that the oxidation of particle components is necessary for luminescence to occur. The characteristics of the emission spectrum as well as the finding that granulocytes activated by a nonparticulate agent (F-) fail to liminesce show that light emission by the relaxation of singlet oxygen to the ground state does not contribute in a major way to the chemiluminescence of phagocytosing granulocytes; whether singlet oxygen contributes to chemiluminescence in other ways cannot be decided from the data available. Inasmuch as the oxidation of constituents of ingested particles is an important bacterial killing mechanism in the granulocyte, chemiluminescence may be viewed as a manifestation of the microbicidal activity of the cell.", "contents": "The origin of the chemiluminescence of phagocytosing granulocytes. Granulocytes engaged in the phagocytosis of opsonized zymosan emit light by a process that is inhibited by superoxide dismutase and catalase. In the present report is is shown that light emission is the result of reactions between certain unspecified constituents of the ingested particles and some or all of the oxidizing agents (H2O2, O2),and possibly the hydroxyl radical and singlet oxygen) produced by the activated cells. This conclusion is based on a study of light emission by both activated cells ans artificial O2 generating system containing xanthine oxidase and purine. With these two systems light production required the presence of both zymosan and oxidizing agent, suggesting that the oxidation of particle components is necessary for luminescence to occur. The characteristics of the emission spectrum as well as the finding that granulocytes activated by a nonparticulate agent (F-) fail to liminesce show that light emission by the relaxation of singlet oxygen to the ground state does not contribute in a major way to the chemiluminescence of phagocytosing granulocytes; whether singlet oxygen contributes to chemiluminescence in other ways cannot be decided from the data available. Inasmuch as the oxidation of constituents of ingested particles is an important bacterial killing mechanism in the granulocyte, chemiluminescence may be viewed as a manifestation of the microbicidal activity of the cell.", "PMID": 965486}
{"id": "pubmed23n0032_6369", "title": "Mechanisms of methylene blue stimulation of the hexose monophosphate shunt in erythrocytes.", "content": "The response of the hexose monophosphate shunt in erythrocytes was studied with the ionization chamber-electrometer apparatus to measure continuously 14CO2 derived from 14C-labeled substrates. The effect of methylene blue at high (0.1 mM) and low (1 muM) concentrations was evaluated under different gas mixtures; air, carbon monoxide, and 6% carbon monoxide in air. The latter gas mixture results in nearly 100% carboxyhemoglobin but provides a physiologic partial pressure of oxygen. The extent to which pentose is recycled through the shunt in response to methylene blue stimulation was examined with radioactive glucose substrates labeled on the first, second, and third carbon positions. Generation of hydrogen peroxide after stimulation of erythrocytes with methylene blue was evaluated by the catalase-aminotriazole trapping technique, [14C]formate oxidation, and oxidation of reduced glutatione. Stimulation of the shunt with 1 muM methylene blue was markedly impaired in the absence of oxyhemoglobin, but stimulation with 0.1 mM methylene blue was only slightly impaired under the carbon monoxide-air mixture. The higher concentration of methylene blue produced evidence of hydrogen peroxide generation of all three techniques. Despite the evidence for the involvement of oxygen, oxyhemoglobin, and hydrogen peroxide in the response to methylene blue, cells containing methemoglobin induced by sodium nitrite or from a patient with congenital methemoglobinemia responded normally to methylene blue in the absence of oxygen. These experiments indicate that the reactions induced by methylene blue in erythrocytes are more complex than generally thought and that high concentrations are associated with production of peroxide.", "contents": "Mechanisms of methylene blue stimulation of the hexose monophosphate shunt in erythrocytes. The response of the hexose monophosphate shunt in erythrocytes was studied with the ionization chamber-electrometer apparatus to measure continuously 14CO2 derived from 14C-labeled substrates. The effect of methylene blue at high (0.1 mM) and low (1 muM) concentrations was evaluated under different gas mixtures; air, carbon monoxide, and 6% carbon monoxide in air. The latter gas mixture results in nearly 100% carboxyhemoglobin but provides a physiologic partial pressure of oxygen. The extent to which pentose is recycled through the shunt in response to methylene blue stimulation was examined with radioactive glucose substrates labeled on the first, second, and third carbon positions. Generation of hydrogen peroxide after stimulation of erythrocytes with methylene blue was evaluated by the catalase-aminotriazole trapping technique, [14C]formate oxidation, and oxidation of reduced glutatione. Stimulation of the shunt with 1 muM methylene blue was markedly impaired in the absence of oxyhemoglobin, but stimulation with 0.1 mM methylene blue was only slightly impaired under the carbon monoxide-air mixture. The higher concentration of methylene blue produced evidence of hydrogen peroxide generation of all three techniques. Despite the evidence for the involvement of oxygen, oxyhemoglobin, and hydrogen peroxide in the response to methylene blue, cells containing methemoglobin induced by sodium nitrite or from a patient with congenital methemoglobinemia responded normally to methylene blue in the absence of oxygen. These experiments indicate that the reactions induced by methylene blue in erythrocytes are more complex than generally thought and that high concentrations are associated with production of peroxide.", "PMID": 965487}
{"id": "pubmed23n0032_6370", "title": "Urate crystal-induced chemotactic factor: isolation and partial characterization.", "content": "A factor with chemotactic properties for neutrophils and mononuclear cells was extracted from the lysosomal fraction of both human and rabbit neutrophils that had been allowed to phagocytose monosodium urate crystals. The chemotactic factor was found to be a glycoprotein with a mol wt of 8,400 daltons. The factor is heat labile and has chemotactic activity for human as well as rabbit cells. Preincubation of the cells with the urate induced chemotactic factor or with complement activated plasma prevents the cell from migrating chemotactically when challenged with either factor in the chemotactic chamber. The chemotactic factor induces release of lysosomal enzymes for cytochalasin B treated human neutrophils.", "contents": "Urate crystal-induced chemotactic factor: isolation and partial characterization. A factor with chemotactic properties for neutrophils and mononuclear cells was extracted from the lysosomal fraction of both human and rabbit neutrophils that had been allowed to phagocytose monosodium urate crystals. The chemotactic factor was found to be a glycoprotein with a mol wt of 8,400 daltons. The factor is heat labile and has chemotactic activity for human as well as rabbit cells. Preincubation of the cells with the urate induced chemotactic factor or with complement activated plasma prevents the cell from migrating chemotactically when challenged with either factor in the chemotactic chamber. The chemotactic factor induces release of lysosomal enzymes for cytochalasin B treated human neutrophils.", "PMID": 965488}
{"id": "pubmed23n0032_6371", "title": "Red cell age-related changes of hemoglobins AIa+b and AIc in normal and diabetic subjects.", "content": "The minor hemoglobin components, hemoglobin AIa+b and hemoglobin AIc, were measured in the 10% youngest and 10% oldest erythrocytes of 15 normal and 14 diabetic subjects. Erythrocyte fractions were obtained by centrifugation in isopyknic concentrations of dextran: 28.5% of 40,000-mol wt dextran yeilded the 10% lightest of young cells, and 30.5% dextran provided the 10% heaviest or old erythrocytes. Both normal and diabetic erythrocytes contain increased amounts of Hb AIa+b and Hb AIc in old as compared to young cells. In normal subjects, young cells contained 1.2+/-0.2%, and old cells contained 1.8+/-0.4% Hb AIa+b. Corresponding values for diabetic cells were 1.7+/-0.6 and 2.6+/-0.9%. Hb AIc increased from 3.1+/-0.8 to 6.0+/-1.1% in normals and from 5.1+/-2.1 to 10.1+/-3.7% in diabetics. The results indicate that both cell age and diabetes are significant determinants of the amounts of Hb AIa+b and Hb AIc.", "contents": "Red cell age-related changes of hemoglobins AIa+b and AIc in normal and diabetic subjects. The minor hemoglobin components, hemoglobin AIa+b and hemoglobin AIc, were measured in the 10% youngest and 10% oldest erythrocytes of 15 normal and 14 diabetic subjects. Erythrocyte fractions were obtained by centrifugation in isopyknic concentrations of dextran: 28.5% of 40,000-mol wt dextran yeilded the 10% lightest of young cells, and 30.5% dextran provided the 10% heaviest or old erythrocytes. Both normal and diabetic erythrocytes contain increased amounts of Hb AIa+b and Hb AIc in old as compared to young cells. In normal subjects, young cells contained 1.2+/-0.2%, and old cells contained 1.8+/-0.4% Hb AIa+b. Corresponding values for diabetic cells were 1.7+/-0.6 and 2.6+/-0.9%. Hb AIc increased from 3.1+/-0.8 to 6.0+/-1.1% in normals and from 5.1+/-2.1 to 10.1+/-3.7% in diabetics. The results indicate that both cell age and diabetes are significant determinants of the amounts of Hb AIa+b and Hb AIc.", "PMID": 965489}
{"id": "pubmed23n0032_6372", "title": "Idiopathic scoliosis. Gas exchange and the age dependence of arterial blood gases.", "content": "The aims were to examine the gas exchange and arterial blood gas abnormalities among patients with scoliosis, and the correlation of these abnormalities with age and severity of deformity. Means among 51 patients were as follows: age 25.4 +/- 17.5 yr, angle of scoliosis 80.2 +/- 29.9 (SD), vital capacity 1.94 +/- 0.91 (SD) (i.e. 60.6 +/- 19.2% of predicted), PaO2 85.8 +/- 12.0 (SD), PaCO2 42.4 +/- 8.0, physiological dead space to tidal volume ratio 0.438 +/- 0.074 (SD), and alveolar-arterial oxygen difference breathing air 14.9 +/- 8.9 (SD). Statistically significant correlations were as follows: the PaCO2 and physiological dead space to tidal volume ratio increased with age, and the PaO2 and alveolar ventilation decreased with age. The PaO2, alveolar ventilation, and tidal volume were inversely related to the angle of scoliosis and directly related to the vital capacity, precent predicted vital capacity, and the compliance of the respiratory system. The physiological dead space to tidal volume ratio and the alveolar-arterial oxygen difference were inversely related to the vital capacity, percent predicted vital capacity, and the compliance of the respiratory system. PaCO2 was directly related to the elastance of the respiratory system. We conclude that ventilation-blood flow maldistribution as a result of deformity of the rib cage was the primary abnormality in gas exchange, and that with age there was progressive deterioration in gas exchange. The age-dependent increase in PaCO2 and decrease in alveolar ventilation were due to the increasing physiological dead space to tidal volume ratio and failure of a compensatory increase in ventilation.", "contents": "Idiopathic scoliosis. Gas exchange and the age dependence of arterial blood gases. The aims were to examine the gas exchange and arterial blood gas abnormalities among patients with scoliosis, and the correlation of these abnormalities with age and severity of deformity. Means among 51 patients were as follows: age 25.4 +/- 17.5 yr, angle of scoliosis 80.2 +/- 29.9 (SD), vital capacity 1.94 +/- 0.91 (SD) (i.e. 60.6 +/- 19.2% of predicted), PaO2 85.8 +/- 12.0 (SD), PaCO2 42.4 +/- 8.0, physiological dead space to tidal volume ratio 0.438 +/- 0.074 (SD), and alveolar-arterial oxygen difference breathing air 14.9 +/- 8.9 (SD). Statistically significant correlations were as follows: the PaCO2 and physiological dead space to tidal volume ratio increased with age, and the PaO2 and alveolar ventilation decreased with age. The PaO2, alveolar ventilation, and tidal volume were inversely related to the angle of scoliosis and directly related to the vital capacity, precent predicted vital capacity, and the compliance of the respiratory system. The physiological dead space to tidal volume ratio and the alveolar-arterial oxygen difference were inversely related to the vital capacity, percent predicted vital capacity, and the compliance of the respiratory system. PaCO2 was directly related to the elastance of the respiratory system. We conclude that ventilation-blood flow maldistribution as a result of deformity of the rib cage was the primary abnormality in gas exchange, and that with age there was progressive deterioration in gas exchange. The age-dependent increase in PaCO2 and decrease in alveolar ventilation were due to the increasing physiological dead space to tidal volume ratio and failure of a compensatory increase in ventilation.", "PMID": 965490}
{"id": "pubmed23n0032_6373", "title": "Regulation of angiotensin II receptors in the rat adrenal cortex by dietary electrolytes.", "content": "The binding affinity and concentration of specific angiotensin II receptor sites of rat adrenal cortical cells and homogenates were determined after 1 and 6 wk of altered sodium and potassium intake. Sodium deprivation caused marked increases in plasma renin, blood angiotensin II, and plasma aldosterone, and was accompanied by a significant increase (+74%) in the number of specific angiotensin II receptor sites per adrenal cortical cell. High potassium intake was followed by increased serum potassium and markedly elevated plasma aldosterone, with subnormal levels of renin and angiotensin II and a 170% increase in the number of angiotensin II receptors per cell after 1 wk. Sodium loading and potassium deprivation were followed by the opposite effect upon adrenal receptors, with reduction of the angiotensin II-binding capacity. None of the dietary electrolyte changes were accompanied by an ancrease in receptor affinity above the control value of 2 nM-1. A decrease in receptor affinity was noted after 6 wk of either low sodium or low potassium intake, when the renin and angiotensin II levels were increased by 104-129%. The adrenals of normal rats infused acutely with synthetic angiotensin II, or anesthetized with ether or sodium pentobarbital, which markedly increased plasma renin activity, contained fewer angiotensin receptors. These reductions in binding site concentration were not accompanied by changes in affinity and were attributed to occupancy by angiotensin II. These studies have demonstrated that chronic changes in sodium or potassium balance and acute changes in blood angiotensin II levels can exert modulating effects upon the adrenal content and/or affinity of specific receptor sites for angiotensin II.", "contents": "Regulation of angiotensin II receptors in the rat adrenal cortex by dietary electrolytes. The binding affinity and concentration of specific angiotensin II receptor sites of rat adrenal cortical cells and homogenates were determined after 1 and 6 wk of altered sodium and potassium intake. Sodium deprivation caused marked increases in plasma renin, blood angiotensin II, and plasma aldosterone, and was accompanied by a significant increase (+74%) in the number of specific angiotensin II receptor sites per adrenal cortical cell. High potassium intake was followed by increased serum potassium and markedly elevated plasma aldosterone, with subnormal levels of renin and angiotensin II and a 170% increase in the number of angiotensin II receptors per cell after 1 wk. Sodium loading and potassium deprivation were followed by the opposite effect upon adrenal receptors, with reduction of the angiotensin II-binding capacity. None of the dietary electrolyte changes were accompanied by an ancrease in receptor affinity above the control value of 2 nM-1. A decrease in receptor affinity was noted after 6 wk of either low sodium or low potassium intake, when the renin and angiotensin II levels were increased by 104-129%. The adrenals of normal rats infused acutely with synthetic angiotensin II, or anesthetized with ether or sodium pentobarbital, which markedly increased plasma renin activity, contained fewer angiotensin receptors. These reductions in binding site concentration were not accompanied by changes in affinity and were attributed to occupancy by angiotensin II. These studies have demonstrated that chronic changes in sodium or potassium balance and acute changes in blood angiotensin II levels can exert modulating effects upon the adrenal content and/or affinity of specific receptor sites for angiotensin II.", "PMID": 965491}
{"id": "pubmed23n0032_6374", "title": "Inherited deficiency of the second component of complement. Rheumatic disease associations.", "content": "The prevalence of homozygous and heterozygous deficiency of the second component of complement (C2) was determined in patients with rheumatic disease including 137 with systemic lupus erythematosus (SLE), 274 with juvenile rheumatoid arthritis, and 134 with rheumatoid arthritis. 1 C2 homozygous deficient and 19 possible heterozygous deficient individuals were identified by using both immunochemical and functional assays to determine C2 levels. Of the 20, 8 had SLE (5.9%), 10 had juvenile rheumatoid arthritis (3.7%), and 2 had rheumatoid arthritis (1.4%), the homozygous deficient individual having SLE. The prevalence of C2 deficiency in the SLE and juvenile rheumatoid arthritis patients was significantly increased (P = 0.0009 and P = 0.02, respectively) when compared with controls, 6 (1.2%) of 509 blood donors having C2 levels consistent with heterozygous deficiency. 15 of the 20 C2 deficient patients were HLA typed and found to have antigens A10(Aw25), B18, or both. The patients with C2 deficiency and SLE had earlier age of onset of disease and less antinuclear antibody when compared with the C2 normal SLE patients. 11 families of the propositi were studied and found to have one or more C2 heterozygous deficient individuals. The family members had an equal distribution of rheumatic disease and antinuclear antibody in the C2 deficient and C2 normal groups. C2 deficient individuals were found to have significantly lower levels of properdin Factor B (242 mug/ml+/-54) when compared with the non-C2 deficient family members (282 mug/ml+/-73). These data support the concept that inherited deficiency of C2 is significantly associated with both SLE and juvenile rheumatoid arthritis.", "contents": "Inherited deficiency of the second component of complement. Rheumatic disease associations. The prevalence of homozygous and heterozygous deficiency of the second component of complement (C2) was determined in patients with rheumatic disease including 137 with systemic lupus erythematosus (SLE), 274 with juvenile rheumatoid arthritis, and 134 with rheumatoid arthritis. 1 C2 homozygous deficient and 19 possible heterozygous deficient individuals were identified by using both immunochemical and functional assays to determine C2 levels. Of the 20, 8 had SLE (5.9%), 10 had juvenile rheumatoid arthritis (3.7%), and 2 had rheumatoid arthritis (1.4%), the homozygous deficient individual having SLE. The prevalence of C2 deficiency in the SLE and juvenile rheumatoid arthritis patients was significantly increased (P = 0.0009 and P = 0.02, respectively) when compared with controls, 6 (1.2%) of 509 blood donors having C2 levels consistent with heterozygous deficiency. 15 of the 20 C2 deficient patients were HLA typed and found to have antigens A10(Aw25), B18, or both. The patients with C2 deficiency and SLE had earlier age of onset of disease and less antinuclear antibody when compared with the C2 normal SLE patients. 11 families of the propositi were studied and found to have one or more C2 heterozygous deficient individuals. The family members had an equal distribution of rheumatic disease and antinuclear antibody in the C2 deficient and C2 normal groups. C2 deficient individuals were found to have significantly lower levels of properdin Factor B (242 mug/ml+/-54) when compared with the non-C2 deficient family members (282 mug/ml+/-73). These data support the concept that inherited deficiency of C2 is significantly associated with both SLE and juvenile rheumatoid arthritis.", "PMID": 965492}
{"id": "pubmed23n0032_6375", "title": "The effect of D-penicillamine on polymorphonuclear leukocyte function.", "content": "D-Penicillamine, a reducing and chelating agent used in the treating of rheumatoid arthritis, was tested for its effects of polymorphonuclear leukocyte chemotaxis, phagocytosis, and lysosomal enzymes. beta-Glucuronidase release from polymorphonuclear leukocytes after phagocytosis of latex particles was not affected by D-penicillamine at concentrations ranging from 25 to 400 mg/liter. No direct effect was seen on enzyme activity at the maximum concentration of the drug. There was no inhibition of latex particle ingestion. No cell damage was found at 400 mg/liter penicillamine as measured by lactic dehydrogenase release. At this drug concentration there was only a 15% reduction in hemolytic complement levels. Chemotaxis was significantly decreased at concentrations of 50 mg/liter with a dose-dependent effect at higher concentrations which showed a plateau from 200 to 400 mg/liter. The parent compound D-cysteine was also tested in these systems. The same lack of effect of phagocytosis and enzyme release was found. D-Cysteine did inhibit chemotaxis but to a lesser degree than D-penicillamine. This dicotomy of drug effect may indicate that the beneficial action of D-penicillamine in the treatment of rheumatoid arthritis is due to the decreased chemotaxis of polymorphonuclear leukocytes into the joint, while the absence of an effect of phagocytosis and lysosomal enzymes shows the cells can still function to ingest and destroy bacteria. This latter effect correlates with the absence of infection in patients treated with this compound.", "contents": "The effect of D-penicillamine on polymorphonuclear leukocyte function. D-Penicillamine, a reducing and chelating agent used in the treating of rheumatoid arthritis, was tested for its effects of polymorphonuclear leukocyte chemotaxis, phagocytosis, and lysosomal enzymes. beta-Glucuronidase release from polymorphonuclear leukocytes after phagocytosis of latex particles was not affected by D-penicillamine at concentrations ranging from 25 to 400 mg/liter. No direct effect was seen on enzyme activity at the maximum concentration of the drug. There was no inhibition of latex particle ingestion. No cell damage was found at 400 mg/liter penicillamine as measured by lactic dehydrogenase release. At this drug concentration there was only a 15% reduction in hemolytic complement levels. Chemotaxis was significantly decreased at concentrations of 50 mg/liter with a dose-dependent effect at higher concentrations which showed a plateau from 200 to 400 mg/liter. The parent compound D-cysteine was also tested in these systems. The same lack of effect of phagocytosis and enzyme release was found. D-Cysteine did inhibit chemotaxis but to a lesser degree than D-penicillamine. This dicotomy of drug effect may indicate that the beneficial action of D-penicillamine in the treatment of rheumatoid arthritis is due to the decreased chemotaxis of polymorphonuclear leukocytes into the joint, while the absence of an effect of phagocytosis and lysosomal enzymes shows the cells can still function to ingest and destroy bacteria. This latter effect correlates with the absence of infection in patients treated with this compound.", "PMID": 965493}
{"id": "pubmed23n0032_6376", "title": "Maximal oxygen consumption in patients with lung disease.", "content": "A theoretical model for oxygen transport assuming a series linkage of ventilation, diffusion, oxygen uptake by erythrocytes, cardiac output, and oxygen release was used to calculate expected values for maximal oxygen intake (VO2max) of patients with various pulmonary disorders 22 patients with either restrictive or obstructive ventilatory impairment were studied at rest and maximal exercise. When exercise measurements of maximal pulmonary blood flow (QCmax), oxygen capacity, membrane diffusing capacity for CO, pulmonary capillary blood volume, alveolar ventilation, and mixed venous oxygen saturation were employed as input values, predictions of VO2max from the model correlated closely with measured values (r = 0.978). Measured VO2max was 976+/-389 ml/min (45.3+/-13% of predicted normal), and VO2max predicted from the model was 1,111+/-427 ml/min. The discrepancy may in part reflect uneven matching of alveolar ventilation, pulmonary capillary blood flow, and membrane diffusing capacity for CO within the lung; uniform matching is assumed in the model so that mismatching will impair gas exchange beyond our predictions. Although QCmax was less than predicted in most patients (63.6+/-19.6% of predicted) the model suggests that raising QCmax to normal could have raised VO2max only 11.6+/-8.8% in the face of existent impairment of intrapulmonary gas exchange. Since pulmonary functions measured at rest correlated well with exercise parameters needed in the model to predict VO2max we developed a nomogram for predicting VO2max from resting CO diffusing capacity, the forced one second expired volume, and the resting ratio of dead space to tidal volume. The correlation coefficient between measured and predicted VO2max, by using this nomogram, was 0.942.", "contents": "Maximal oxygen consumption in patients with lung disease. A theoretical model for oxygen transport assuming a series linkage of ventilation, diffusion, oxygen uptake by erythrocytes, cardiac output, and oxygen release was used to calculate expected values for maximal oxygen intake (VO2max) of patients with various pulmonary disorders 22 patients with either restrictive or obstructive ventilatory impairment were studied at rest and maximal exercise. When exercise measurements of maximal pulmonary blood flow (QCmax), oxygen capacity, membrane diffusing capacity for CO, pulmonary capillary blood volume, alveolar ventilation, and mixed venous oxygen saturation were employed as input values, predictions of VO2max from the model correlated closely with measured values (r = 0.978). Measured VO2max was 976+/-389 ml/min (45.3+/-13% of predicted normal), and VO2max predicted from the model was 1,111+/-427 ml/min. The discrepancy may in part reflect uneven matching of alveolar ventilation, pulmonary capillary blood flow, and membrane diffusing capacity for CO within the lung; uniform matching is assumed in the model so that mismatching will impair gas exchange beyond our predictions. Although QCmax was less than predicted in most patients (63.6+/-19.6% of predicted) the model suggests that raising QCmax to normal could have raised VO2max only 11.6+/-8.8% in the face of existent impairment of intrapulmonary gas exchange. Since pulmonary functions measured at rest correlated well with exercise parameters needed in the model to predict VO2max we developed a nomogram for predicting VO2max from resting CO diffusing capacity, the forced one second expired volume, and the resting ratio of dead space to tidal volume. The correlation coefficient between measured and predicted VO2max, by using this nomogram, was 0.942.", "PMID": 965494}
{"id": "pubmed23n0032_6377", "title": "The steady state concentration gradients of an electron-dense marker (ferritin in the three-layered hemochorial placenta of the rabbit.", "content": "Ferritin was injected into the fetal or the maternal circulation of 27-29-day-pregnant rabbits. After the occurrence of a quasi-steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the two interstitial compartments of the three-layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces \"excluded\" ferritin; the plasma-interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto- and syncytiotrophoblasts. 55% of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45% across the cytotrophoblast, and less than 5% across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these three layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these three layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.", "contents": "The steady state concentration gradients of an electron-dense marker (ferritin in the three-layered hemochorial placenta of the rabbit. Ferritin was injected into the fetal or the maternal circulation of 27-29-day-pregnant rabbits. After the occurrence of a quasi-steady state, the placentas were prepared for electron microscopy. Ferritin particles were counted in the electron micrographs in the fetal capillaries, in the maternal blood spaces, and in the two interstitial compartments of the three-layered placenta. Under the circumstances of the experiments (excessively elevated plasma ferritin concentrations), no evidence was found for nondiffusional transport of radiolabeled ferritin. Comparison of the standing concentration gradients in the placentas, recorded after maternal and after fetal injection, showed that the interstitial spaces \"excluded\" ferritin; the plasma-interstitial space ferritin partition coefficients were 10 in the basement membrane space and 3 in the space between the cyto- and syncytiotrophoblasts. 55% of the total concentration gradient across the rabbit placenta occurred across the fetal endothelium, about 45% across the cytotrophoblast, and less than 5% across the syncytiotrophoblast. These figures are believed to reflect the relative contributions of these three layers to the total diffusional resistance in the rabbit placenta. When compared to previous data on the relative contributions of these three layers for small ions and molecules, the present data lead to the conclusion that discrimination of molecular size is a function of the fetal capillary endothelium alone.", "PMID": 965495}
{"id": "pubmed23n0032_6378", "title": "Lead poisoning: association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5'-nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines.", "content": "Lead intoxication is accompanied by an acquired deficiency of erythrocyte pryimidine-specific, 5'-nucleotidase. Genetically determined deficiency of this enzyme is associated with chronic hemolysis, marked basophilic stippling of erythrocytes on stained blood films, and unique intraerythrocytic accumulations of pyrimidine-containing nucleotides. The present report documents that lead-induced deficiency when sufficiently severe gives rise to findings similar to the hereditary disorder. Whereas pyrimidine-containing nucleotides are virutally absent in the erythrocytes of normal and reticulocyte-rich blood, 12% of erythrocyte nucloetides in the blood of a patient with lead intoxication contained cytidine. Nucleotidase activity was about 25% that in normal erythrocytes and 15% or less of that expected in comparable reticulocyte-rich blood. The distribution of nucleotidase activity in patient erythrocytes is unknown, and much more severe deficiency could have been present in subsets of the cell populations analyzed. The findings indicate that the hemolytic anemia and increased basophilic stippling characteristic of certain cases of lead intoxication may share a common etiology with essentially identical features of the genetically determined disorder.", "contents": "Lead poisoning: association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5'-nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines. Lead intoxication is accompanied by an acquired deficiency of erythrocyte pryimidine-specific, 5'-nucleotidase. Genetically determined deficiency of this enzyme is associated with chronic hemolysis, marked basophilic stippling of erythrocytes on stained blood films, and unique intraerythrocytic accumulations of pyrimidine-containing nucleotides. The present report documents that lead-induced deficiency when sufficiently severe gives rise to findings similar to the hereditary disorder. Whereas pyrimidine-containing nucleotides are virutally absent in the erythrocytes of normal and reticulocyte-rich blood, 12% of erythrocyte nucloetides in the blood of a patient with lead intoxication contained cytidine. Nucleotidase activity was about 25% that in normal erythrocytes and 15% or less of that expected in comparable reticulocyte-rich blood. The distribution of nucleotidase activity in patient erythrocytes is unknown, and much more severe deficiency could have been present in subsets of the cell populations analyzed. The findings indicate that the hemolytic anemia and increased basophilic stippling characteristic of certain cases of lead intoxication may share a common etiology with essentially identical features of the genetically determined disorder.", "PMID": 965496}
{"id": "pubmed23n0032_6379", "title": "The role of complement in the clearance of cold agglutinin-sensitized erythrocytes in man.", "content": "To define the pathophysiologic mechanisms of cold agglutinin disease, we investigated a human model of this syndrome in normal volunteers and in patients with diminished levels of serum complement. Subjects received intravenous injections of autologous, chromated (51Cr) erythrocytes which had been exposed in vitro to purified cold agglutinin preparations and to fresh autologous serum (as a source of complement). In vitro tests confirmed that such cells were coated with activated complement components (C3b), but not with immunoglobulin. Studies of erythrocyte clearance and simultaneous organ scanning showed that erythrocytes sensitized with low levels of cold agglutinin primarily undergo reticuloendothelial sequestration by the liver rather than intravascular hemolysis. After the initial sequestration of C3b-coated erythrocytes, a fraction of the cells are released back into the circulation and survive normally thereafter. Both phenomena are dose dependent and closely follow the sequestration and release pattern observed with IgM isoagglutinin sensitization. Experiments that used heated autologous serum as a source of B3 inactivator demonstrated that functionally intact C3b is required for hepatic sequestration. Erythrocytes coated with C3d were not cleared from the circulation. In vitro assays that sued human macrophage monolayers suggested that the intrahepatic conversion of C3b to C3d is responsible for the release of sensitized erythrocytes back into the circulation. The clearance of cold agglutinin-sensititzed erythrocytes was compared to the clearance mediated by IgM isoagglutinin. We found that the rate of complement fixation by an IgM antibody proceeds rapidly in vivo that the time for complement activation is not a factor in limiting the rate of hepatic sequestration. The major limiting factor appears to be the rate of liver blood flow. Maximal in vitro coating of erythrocytes with C3d conferred protection from further cold agglutinin sensitization but not from IgM isoagglutinin-mediated clearance. This suggests a mechanism for the resistance to lysis observed in cells obtained from patients with the cold agglutinin syndrome and confirms the marked dependence of the site of C3 attachment on the site of membrane localization of the sensitizing antibody.", "contents": "The role of complement in the clearance of cold agglutinin-sensitized erythrocytes in man. To define the pathophysiologic mechanisms of cold agglutinin disease, we investigated a human model of this syndrome in normal volunteers and in patients with diminished levels of serum complement. Subjects received intravenous injections of autologous, chromated (51Cr) erythrocytes which had been exposed in vitro to purified cold agglutinin preparations and to fresh autologous serum (as a source of complement). In vitro tests confirmed that such cells were coated with activated complement components (C3b), but not with immunoglobulin. Studies of erythrocyte clearance and simultaneous organ scanning showed that erythrocytes sensitized with low levels of cold agglutinin primarily undergo reticuloendothelial sequestration by the liver rather than intravascular hemolysis. After the initial sequestration of C3b-coated erythrocytes, a fraction of the cells are released back into the circulation and survive normally thereafter. Both phenomena are dose dependent and closely follow the sequestration and release pattern observed with IgM isoagglutinin sensitization. Experiments that used heated autologous serum as a source of B3 inactivator demonstrated that functionally intact C3b is required for hepatic sequestration. Erythrocytes coated with C3d were not cleared from the circulation. In vitro assays that sued human macrophage monolayers suggested that the intrahepatic conversion of C3b to C3d is responsible for the release of sensitized erythrocytes back into the circulation. The clearance of cold agglutinin-sensititzed erythrocytes was compared to the clearance mediated by IgM isoagglutinin. We found that the rate of complement fixation by an IgM antibody proceeds rapidly in vivo that the time for complement activation is not a factor in limiting the rate of hepatic sequestration. The major limiting factor appears to be the rate of liver blood flow. Maximal in vitro coating of erythrocytes with C3d conferred protection from further cold agglutinin sensitization but not from IgM isoagglutinin-mediated clearance. This suggests a mechanism for the resistance to lysis observed in cells obtained from patients with the cold agglutinin syndrome and confirms the marked dependence of the site of C3 attachment on the site of membrane localization of the sensitizing antibody.", "PMID": 965497}
{"id": "pubmed23n0032_6380", "title": "Irreversible deformation of the spectrin-actin lattice in irreversibly sickled cells.", "content": "Irreversibly sickled cells (ISC's) are circulating erythrocytes in patients with sickle cell disease that retain a sickled shape even when oxygenated. Evidence points to a membrane defect that prevents the return of these cells to the normal biconcave shape. The erythrocyte membrane protein spectrin is believed to help control erythrocyte shape and deformability. Recent studies suggest that normally spectrin and an erythrocyte actin form a self-supporting, fibrillar, lattice-like network on the cytoplasmic membrane surface. When normal erythrocyte ghosts are extracted with Triton X-100 all the integral membrane proteins and most of the membrane lipids are removed, leaving a ghost-shaped residue composed principally of spectrin and actin. We concentrated ISC's from patients with sickle cell anemia and compared the morphology and protein composition of ghosts and Triton-extracted ghost residues prepared from these ISC's with similar preparations of reversibly sickable cells and normal cells. (a) Many ISC's formed ISC-shaped ghosts. (b) All ISC-shaped ghosts formed ISC-shaped Triton residues. (c) Spectrin, erythrocyte actin (Band 5), an unidentified Band 3 component, and Band 4.1 were the major protein components of the Triton residues. All membrane-associated sickle hemoglobin was removed by the Triton treatment. (d) No ISC-shaped ghosts or ISC-shaped Triton residues were formed when deoxygenated, sickled RSC's were lysed or Triton-extracted. ISC-shaped ghosts and Triton residues were never formed from normal cells. These observations suggest that a defect of the \"spectrin-actin lattice\" may be the primary abnormality of the ISC membrane. Since ISC's are rigid cells, the data support the postulate that spectrin is a major determinant of membrane deformability. Finally, they provide direct evidence that spectrin is important in determining erythrocyte shape.", "contents": "Irreversible deformation of the spectrin-actin lattice in irreversibly sickled cells. Irreversibly sickled cells (ISC's) are circulating erythrocytes in patients with sickle cell disease that retain a sickled shape even when oxygenated. Evidence points to a membrane defect that prevents the return of these cells to the normal biconcave shape. The erythrocyte membrane protein spectrin is believed to help control erythrocyte shape and deformability. Recent studies suggest that normally spectrin and an erythrocyte actin form a self-supporting, fibrillar, lattice-like network on the cytoplasmic membrane surface. When normal erythrocyte ghosts are extracted with Triton X-100 all the integral membrane proteins and most of the membrane lipids are removed, leaving a ghost-shaped residue composed principally of spectrin and actin. We concentrated ISC's from patients with sickle cell anemia and compared the morphology and protein composition of ghosts and Triton-extracted ghost residues prepared from these ISC's with similar preparations of reversibly sickable cells and normal cells. (a) Many ISC's formed ISC-shaped ghosts. (b) All ISC-shaped ghosts formed ISC-shaped Triton residues. (c) Spectrin, erythrocyte actin (Band 5), an unidentified Band 3 component, and Band 4.1 were the major protein components of the Triton residues. All membrane-associated sickle hemoglobin was removed by the Triton treatment. (d) No ISC-shaped ghosts or ISC-shaped Triton residues were formed when deoxygenated, sickled RSC's were lysed or Triton-extracted. ISC-shaped ghosts and Triton residues were never formed from normal cells. These observations suggest that a defect of the \"spectrin-actin lattice\" may be the primary abnormality of the ISC membrane. Since ISC's are rigid cells, the data support the postulate that spectrin is a major determinant of membrane deformability. Finally, they provide direct evidence that spectrin is important in determining erythrocyte shape.", "PMID": 965498}
{"id": "pubmed23n0032_6381", "title": "Mechanisms of portal hypertension-induced alterations in renal hemodynamics, renal water excretion, and renin secretion.", "content": "Clinical states with portal venous hypertension are frequently associated with impairment in renal hemodynamics and water excretion, as well as increased renin secretion. In the present investigation, portal venous pressure (PVP) was increased in anesthetized dogs undergoing a water diuresis. Renal arterial pressure was maintained constant in all studies. As PVP was increased from 6 to 20 mm Hg, decreases in cardiac output (2.5-2.0 liter/min, P less than 0.05) and mean arterial pressure (140-131 mm Hg, P less than 0.05) were observed. Increases in PVP were also associated with decreases in glomerular filtration rate (GFR, 40-31 ml/min, P less than 0.001), renal blood flow (RBF, 276-193 ml/min, P less than  0.001), and increases in renin secretion (232-939 U/min, P less than 0.025) in innervated kidneys. No significant change in either GFR or RBF and a decrease in renin secretion occurred with increases in PVP in denervated kidneys. To dissociate the changes in cardiac output and mean arterial pressure induced by increase PVP from the observed decreases in GFR and RBF, studies were performed on animals undergoing constriction of the thoracic inferior vena cava. In these studies, similar decreases in cardiac output and mean arterial pressure were not associated with significant changes in GFR or RBF. Increases in PVP also were associated with an antidiuresis as urine osmolality increased from 101 to 446 mosmol/kg H2O (P less than 0.001). This antidiuresis was significantly blunted but not abolished by acute hypophysectomy. In hypophysectomized animals, changes in free water clearance and urine flow were linearly correlated as PVP was increased. These studies indicate that increases in PVP result in decreases in GFR and RBF and increases in renin secretion mediated by increased renal adrenergic tone. Increased PVP is also associated with antidiuresis; this antidiuresis is mediated both by vasopressin release and by diminished tubular fluid delivery to the distal nephron.", "contents": "Mechanisms of portal hypertension-induced alterations in renal hemodynamics, renal water excretion, and renin secretion. Clinical states with portal venous hypertension are frequently associated with impairment in renal hemodynamics and water excretion, as well as increased renin secretion. In the present investigation, portal venous pressure (PVP) was increased in anesthetized dogs undergoing a water diuresis. Renal arterial pressure was maintained constant in all studies. As PVP was increased from 6 to 20 mm Hg, decreases in cardiac output (2.5-2.0 liter/min, P less than 0.05) and mean arterial pressure (140-131 mm Hg, P less than 0.05) were observed. Increases in PVP were also associated with decreases in glomerular filtration rate (GFR, 40-31 ml/min, P less than 0.001), renal blood flow (RBF, 276-193 ml/min, P less than  0.001), and increases in renin secretion (232-939 U/min, P less than 0.025) in innervated kidneys. No significant change in either GFR or RBF and a decrease in renin secretion occurred with increases in PVP in denervated kidneys. To dissociate the changes in cardiac output and mean arterial pressure induced by increase PVP from the observed decreases in GFR and RBF, studies were performed on animals undergoing constriction of the thoracic inferior vena cava. In these studies, similar decreases in cardiac output and mean arterial pressure were not associated with significant changes in GFR or RBF. Increases in PVP also were associated with an antidiuresis as urine osmolality increased from 101 to 446 mosmol/kg H2O (P less than 0.001). This antidiuresis was significantly blunted but not abolished by acute hypophysectomy. In hypophysectomized animals, changes in free water clearance and urine flow were linearly correlated as PVP was increased. These studies indicate that increases in PVP result in decreases in GFR and RBF and increases in renin secretion mediated by increased renal adrenergic tone. Increased PVP is also associated with antidiuresis; this antidiuresis is mediated both by vasopressin release and by diminished tubular fluid delivery to the distal nephron.", "PMID": 965499}
{"id": "pubmed23n0032_6382", "title": "Molecular organization of the cholesteryl ester droplets in the fatty streaks of human aorta.", "content": "X-ray diffraction patterns from human arterial specimens containing atherosclerotic fatty streak lesions exhibited a single sharp reflection, corresponding to a structural spacing of about 35 A. Specimens without lesions did not. When specimens with fatty streaks were heated, an order-to-disorder phase transition was revealed by the disappearance of the sharp reflection. The transition was thermally reversible and its temperature varied from aorta to aorta over a range from 28 degrees to 42 degrees C. Since cholesteryl ester droplets are a major component of fatty streaks, comparison studies were made of the diffraction behavior from pure cholesteryl esters. We found that the diffraction patterns of the fatty streak material could be accounted for by the organization of the cholesteryl esters into a liquid-crystalline smectic phase that melts from the smectic to a less ordered phase upon heating. When combined with the conclusions of others from polarized light microscopy, our study shows that a droplet in the smectic phase has well-defined concentric layers of lipid molecules. In each layer, the long axes of the molecules have a net radial orientation with respect to the droplet, but the side-to-side organization is disordered. We suggest that the accessibility of portions of the lipids for specific binding to enzymes or transport proteins may be restricted when they are in the smectic state, and that exchange of lipids with surrounding membranes or other potential binding sites may likewise be inhibited. The restriction in the smectic phase should be greater than in the less ordered phases that exist at higher temperatures.", "contents": "Molecular organization of the cholesteryl ester droplets in the fatty streaks of human aorta. X-ray diffraction patterns from human arterial specimens containing atherosclerotic fatty streak lesions exhibited a single sharp reflection, corresponding to a structural spacing of about 35 A. Specimens without lesions did not. When specimens with fatty streaks were heated, an order-to-disorder phase transition was revealed by the disappearance of the sharp reflection. The transition was thermally reversible and its temperature varied from aorta to aorta over a range from 28 degrees to 42 degrees C. Since cholesteryl ester droplets are a major component of fatty streaks, comparison studies were made of the diffraction behavior from pure cholesteryl esters. We found that the diffraction patterns of the fatty streak material could be accounted for by the organization of the cholesteryl esters into a liquid-crystalline smectic phase that melts from the smectic to a less ordered phase upon heating. When combined with the conclusions of others from polarized light microscopy, our study shows that a droplet in the smectic phase has well-defined concentric layers of lipid molecules. In each layer, the long axes of the molecules have a net radial orientation with respect to the droplet, but the side-to-side organization is disordered. We suggest that the accessibility of portions of the lipids for specific binding to enzymes or transport proteins may be restricted when they are in the smectic state, and that exchange of lipids with surrounding membranes or other potential binding sites may likewise be inhibited. The restriction in the smectic phase should be greater than in the less ordered phases that exist at higher temperatures.", "PMID": 965500}
{"id": "pubmed23n0032_6383", "title": "Psychotic children's free-choice selection of natural and distorted speech.", "content": "The relative preferences of psychotic, language-handicapped, and linguistically normal children for natural intelligible speech or various forms of distorted speech were assessed by a two-choice operant procedure. The stimulus preferences of the psychotic group were found to be qualitatively different from those of either nonpsychotic group. The nonpsychotic children preferred natural intelligible speech in every case, although the language-handicapped children were less sensitive than the normals to the distinctions between communicative speech and unintelligible speech. In contrast, the psychotic group showed an overall preference for unnatural, distorted speech. They apparently attended primarily to the acoustic novelty of the stimuli and had little regard for linguistic intelligibility. These results suggest that speech and language may have distinctly different functional significance for these psychotic children than for normals.", "contents": "Psychotic children's free-choice selection of natural and distorted speech. The relative preferences of psychotic, language-handicapped, and linguistically normal children for natural intelligible speech or various forms of distorted speech were assessed by a two-choice operant procedure. The stimulus preferences of the psychotic group were found to be qualitatively different from those of either nonpsychotic group. The nonpsychotic children preferred natural intelligible speech in every case, although the language-handicapped children were less sensitive than the normals to the distinctions between communicative speech and unintelligible speech. In contrast, the psychotic group showed an overall preference for unnatural, distorted speech. They apparently attended primarily to the acoustic novelty of the stimuli and had little regard for linguistic intelligibility. These results suggest that speech and language may have distinctly different functional significance for these psychotic children than for normals.", "PMID": 965502}
{"id": "pubmed23n0032_6384", "title": "Confusions in recognizing phonemes spoken by esophageal speakers: I. initial consonants and clusters.", "content": "A methodology was developed to determine the patterns of phonemic errors that listeners would make in receiving the initial consonants and clusters of monosyllables spoken by esophageal speakers. Analyses of the features preserved in the errors were performed. The identified phonemic error patterns and the features found to be poorly preserved in the errors were used to structure multiple-choice intelligibility practice materials for esophageal speakers.", "contents": "Confusions in recognizing phonemes spoken by esophageal speakers: I. initial consonants and clusters. A methodology was developed to determine the patterns of phonemic errors that listeners would make in receiving the initial consonants and clusters of monosyllables spoken by esophageal speakers. Analyses of the features preserved in the errors were performed. The identified phonemic error patterns and the features found to be poorly preserved in the errors were used to structure multiple-choice intelligibility practice materials for esophageal speakers.", "PMID": 965503}
{"id": "pubmed23n0032_6385", "title": "Punishment contingencies for the reduction of incorrect responses during articulation instruction.", "content": "A comparison was made between the effects of punishment combined with positive reinforcement and reinforcement alone for the reduction of incorrect articulation responses. Three different punishers (a buzzer, response cost, and \"No!\") were studied for six young children while they were receiving programmed articulation instruction. The results indicated (1) that punishment in combination with positive reinforcement was generally more effective than positive reinforcement alone; (2) that whether or not a particular stimulus acted as a punisher could be determined only be empirical demonstration; and (3) that the introduction of punishment contingencies did not produce increased rates of disruptive, off-task behaviors. Conversely, off-task behaviors tended to increase in rate when punishement contingencies for articulation responses were removed.", "contents": "Punishment contingencies for the reduction of incorrect responses during articulation instruction. A comparison was made between the effects of punishment combined with positive reinforcement and reinforcement alone for the reduction of incorrect articulation responses. Three different punishers (a buzzer, response cost, and \"No!\") were studied for six young children while they were receiving programmed articulation instruction. The results indicated (1) that punishment in combination with positive reinforcement was generally more effective than positive reinforcement alone; (2) that whether or not a particular stimulus acted as a punisher could be determined only be empirical demonstration; and (3) that the introduction of punishment contingencies did not produce increased rates of disruptive, off-task behaviors. Conversely, off-task behaviors tended to increase in rate when punishement contingencies for articulation responses were removed.", "PMID": 965504}
{"id": "pubmed23n0032_6386", "title": "Nasalance in utterances of hearing-impaired speakers.", "content": "Instrumental comparisons of sound from the nose and mouth expressed in percent nasalance, articulation errors, and speech rate are used in this study to contrast utterances of 50 speakers with severe hearing impairment and 64 with normal hearing. Results of the study revealed that 54% of the hearing-impaired subjects had nasalance ratio scores greater than two standard deviations above the mean of the group with normal auditory acuity. No general relationship was found between the number or type of articulation errors and the nasalance scores. Rate of speaking was significantly related to the nasalance scores of the normal group but not those of the hearing-impaired group. Tonagrams displaying the variations in nasalance revealed small, 1 to 50% spike-shaped fluctuations in the displays of the utterances from both groups. Additional prolonged bursts of nasalance in excess of a 30% change in the ratio between the nasal and oral signal were common in the displays from the hearing-impaired group did not in those of the group with normal acuity. Possible sources and perceptual effects of these phenomena are discussed.", "contents": "Nasalance in utterances of hearing-impaired speakers. Instrumental comparisons of sound from the nose and mouth expressed in percent nasalance, articulation errors, and speech rate are used in this study to contrast utterances of 50 speakers with severe hearing impairment and 64 with normal hearing. Results of the study revealed that 54% of the hearing-impaired subjects had nasalance ratio scores greater than two standard deviations above the mean of the group with normal auditory acuity. No general relationship was found between the number or type of articulation errors and the nasalance scores. Rate of speaking was significantly related to the nasalance scores of the normal group but not those of the hearing-impaired group. Tonagrams displaying the variations in nasalance revealed small, 1 to 50% spike-shaped fluctuations in the displays of the utterances from both groups. Additional prolonged bursts of nasalance in excess of a 30% change in the ratio between the nasal and oral signal were common in the displays from the hearing-impaired group did not in those of the group with normal acuity. Possible sources and perceptual effects of these phenomena are discussed.", "PMID": 965505}
{"id": "pubmed23n0032_6387", "title": "Development of acoustic cue discrimination in children.", "content": "Three groups of twelve subjects each, ages four, five and six years, respectively, made same-different judgments on six speech discrimination tests. On each test, a single acoustic cue signaled a phonemic difference in minimal pairs which were otherwise acoustically identical. On three of the tests, the independent variable was a spectral cue and on three others a temporal cue was manipulated. With one exception, all subjects passed tests involving a spectral cue, however, significant developmental differences were apparent on tests in which a temporal cue signaled a phonemic difference. The probable contribution of these cues to the perception of speech in young children is discussed.", "contents": "Development of acoustic cue discrimination in children. Three groups of twelve subjects each, ages four, five and six years, respectively, made same-different judgments on six speech discrimination tests. On each test, a single acoustic cue signaled a phonemic difference in minimal pairs which were otherwise acoustically identical. On three of the tests, the independent variable was a spectral cue and on three others a temporal cue was manipulated. With one exception, all subjects passed tests involving a spectral cue, however, significant developmental differences were apparent on tests in which a temporal cue signaled a phonemic difference. The probable contribution of these cues to the perception of speech in young children is discussed.", "PMID": 965506}
{"id": "pubmed23n0032_6388", "title": "The frequency of disfluencies during phonatory transitions in stuttered and nonstuttered speech.", "content": "The hypothesis that stuttering and nonstuttering adults have fewer disfluencies during voiced-voiced phoneme-to-phoneme phonatory transitions than during voiced-voiceless, voiceless-voiced, or voiceless-voiceless transitions was investigated. The speech of 11 adult stutterers and a matched group of nonstutterers was analyzed according to the occurrence of disfluencies during the above categories of phonatory transtions. Each subject's speech was recorded individually while reading several passages. The percentage of disfluencies varied significantly (p less than 0.001) across the four phonatory transition categories for both groups of subjects. Both stutterers and nonstutterers demonstrated a lower percentage of disfluencies during voiced-voiced transitions than during voiced-voiceless, voiceless-voiced, and voiceless-voiceless phonatory transitions. Furthermore, both groups of subjects demonstrated a similar distribution of disfluencies across the four phonatory transitions categories. An inability to successfully complete phoneme-to-phoneme phonatory transitions does not appear to fully explain the pattern of disfluencies for stuttering or nonstuttering adults.", "contents": "The frequency of disfluencies during phonatory transitions in stuttered and nonstuttered speech. The hypothesis that stuttering and nonstuttering adults have fewer disfluencies during voiced-voiced phoneme-to-phoneme phonatory transitions than during voiced-voiceless, voiceless-voiced, or voiceless-voiceless transitions was investigated. The speech of 11 adult stutterers and a matched group of nonstutterers was analyzed according to the occurrence of disfluencies during the above categories of phonatory transtions. Each subject's speech was recorded individually while reading several passages. The percentage of disfluencies varied significantly (p less than 0.001) across the four phonatory transition categories for both groups of subjects. Both stutterers and nonstutterers demonstrated a lower percentage of disfluencies during voiced-voiced transitions than during voiced-voiceless, voiceless-voiced, and voiceless-voiceless phonatory transitions. Furthermore, both groups of subjects demonstrated a similar distribution of disfluencies across the four phonatory transitions categories. An inability to successfully complete phoneme-to-phoneme phonatory transitions does not appear to fully explain the pattern of disfluencies for stuttering or nonstuttering adults.", "PMID": 965507}
{"id": "pubmed23n0032_6389", "title": "Voice initiation characteristics of normal children and children with vocal nodules: a preliminary investigation.", "content": "Three types of vocal initiation (hard, soft, breathy) were produced by eight normal speaking children and eight children with vocal nodules. The results of the acoustic and aerodynamic measurements indicated systematically longer rise time values and greater air volumes during the initial few hundred milliseconds of phonation for vocal nodule children when compared with the normal speaking children across the three initiation conditions. A significant difference between the two groups was found for the amount of air volume expired in the first 200 msec of hard vocal initiation. Clinical implications of the results suggest reducing the abnormal glottal attack and abusive speaking habits of children with laryngeal dysfunction.", "contents": "Voice initiation characteristics of normal children and children with vocal nodules: a preliminary investigation. Three types of vocal initiation (hard, soft, breathy) were produced by eight normal speaking children and eight children with vocal nodules. The results of the acoustic and aerodynamic measurements indicated systematically longer rise time values and greater air volumes during the initial few hundred milliseconds of phonation for vocal nodule children when compared with the normal speaking children across the three initiation conditions. A significant difference between the two groups was found for the amount of air volume expired in the first 200 msec of hard vocal initiation. Clinical implications of the results suggest reducing the abnormal glottal attack and abusive speaking habits of children with laryngeal dysfunction.", "PMID": 965508}
{"id": "pubmed23n0032_6390", "title": "The cat optic nerve: fibre total count and diameter spectrum.", "content": "An electron microscopic examination of two cat optic nerves indicates a mean total count of 193,000 fibres ranging from 0.5 mu to 13.5 mu in diameter. This count, although nearly double any previously reported, supports recent minimum estimates of the retinal ganglion cell population of the cat eye. A radial gradient of packing density exists across the nerve close to the globe; a high density \"core\" with a unimodal fibre diameter spectrum may be identified as the area centralis outflow and a peripheral low density region with a trimodal diameter spectrum contains the projection of the peripheral retina. Division of the peripheral fibre spectrum suggests the percentages of alpha, beta and gamma ganglion cells in the peripheral retina to be 5%, 42% and 53% respectively.", "contents": "The cat optic nerve: fibre total count and diameter spectrum. An electron microscopic examination of two cat optic nerves indicates a mean total count of 193,000 fibres ranging from 0.5 mu to 13.5 mu in diameter. This count, although nearly double any previously reported, supports recent minimum estimates of the retinal ganglion cell population of the cat eye. A radial gradient of packing density exists across the nerve close to the globe; a high density \"core\" with a unimodal fibre diameter spectrum may be identified as the area centralis outflow and a peripheral low density region with a trimodal diameter spectrum contains the projection of the peripheral retina. Division of the peripheral fibre spectrum suggests the percentages of alpha, beta and gamma ganglion cells in the peripheral retina to be 5%, 42% and 53% respectively.", "PMID": 965509}
{"id": "pubmed23n0032_6391", "title": "Effect of prestimulation on avoidance responding in rats, and hormonal dependence of the effect.", "content": "Rats were tested daily in a free-operant avoidance task and were found to make more avoidance responses in sessions preceded by a brief period of prestimulation than in control sessions. Footshock, air blast, and handling were effective sources of prestimulation for this effect. Further experiments showed that the effect of prestimulation depended on the presence of circulating mineralocorticoid and not on any other adrenal or pituitary hormone. Adrenalectomy, but not hypophysectomy, abolished the effect. The effect was also abolished in intact rats by maintaining them on saline drinking fluid, a procedure that inhibits mineralocorticoid production. In either adrenalectomized rats or intact, saline-maintained rats, injection of mineralocorticoid permitted the effect of prestimulation to reappear while leaving the nonprestimulated response rate unaffected.", "contents": "Effect of prestimulation on avoidance responding in rats, and hormonal dependence of the effect. Rats were tested daily in a free-operant avoidance task and were found to make more avoidance responses in sessions preceded by a brief period of prestimulation than in control sessions. Footshock, air blast, and handling were effective sources of prestimulation for this effect. Further experiments showed that the effect of prestimulation depended on the presence of circulating mineralocorticoid and not on any other adrenal or pituitary hormone. Adrenalectomy, but not hypophysectomy, abolished the effect. The effect was also abolished in intact rats by maintaining them on saline drinking fluid, a procedure that inhibits mineralocorticoid production. In either adrenalectomized rats or intact, saline-maintained rats, injection of mineralocorticoid permitted the effect of prestimulation to reappear while leaving the nonprestimulated response rate unaffected.", "PMID": 965510}
{"id": "pubmed23n0032_6392", "title": "Fornix damage enhances successive, but not simultaneous, object-discrimination learning in cats.", "content": "The purpose of this study was to compare the effects of fornix lesions with the deficits known to follow septum lesions in cats learning visual object discriminations. In Experiment 1 when eight cats with aspiration lesions of the fornix performed successive discrimination problems, acquisition was greatly facilitated, but first- and second-reversal scores were normal by comparison with eight normal cats and two operated corpus callosum-control cats without fornix damage. Four corpus callosum-damaged cats with secondary degeneration in the medial fornix also learned the initial problem faster than normals. In Experiment 2 in a simultaneous paradigm, eight cats with fornix lesions scored like five normal controls, although they perseverated in early stages of reversal problems. The authors hypothesize that medial fornix damage, in contrast with septum lesions, enhances both initiation and maintenance of response inhibition.", "contents": "Fornix damage enhances successive, but not simultaneous, object-discrimination learning in cats. The purpose of this study was to compare the effects of fornix lesions with the deficits known to follow septum lesions in cats learning visual object discriminations. In Experiment 1 when eight cats with aspiration lesions of the fornix performed successive discrimination problems, acquisition was greatly facilitated, but first- and second-reversal scores were normal by comparison with eight normal cats and two operated corpus callosum-control cats without fornix damage. Four corpus callosum-damaged cats with secondary degeneration in the medial fornix also learned the initial problem faster than normals. In Experiment 2 in a simultaneous paradigm, eight cats with fornix lesions scored like five normal controls, although they perseverated in early stages of reversal problems. The authors hypothesize that medial fornix damage, in contrast with septum lesions, enhances both initiation and maintenance of response inhibition.", "PMID": 965511}
{"id": "pubmed23n0032_6393", "title": "Androgenic control of food intake and body weight in male rats.", "content": "Castration of adult male rats produces a delayed (by approximately 1 mo), permanent hypophagia and reduction in weight gain. This contrasts with the rapid, transient hyperphagia and increased weight gain caused by ovariectomy in female rats. Injections of testosterone propionate (TP) stimulate food intake and weight gain in castrated males. Neither 5alpha-reduction nor aromatization of the testosterone molecule plays an important role in the stimulation of these measures by TP. The 5alpha-reduced metabolite of TP, 5alpha-dihydrotestosterone propionate (DHTP), is less effective in increasing eating and weight gain than is TP. Very high doses of TP may actually reduce weight gain with prolonged treatment (2-6 wk). It is likely that this reduced weight gain is due to aromatization of TP to an estrogen. The nonaromatizable androgen, DHTP, does not reduce weight gain even in very high doses, and concurrent progesterone injections reverse the weight-reducing actions of high TP doses.", "contents": "Androgenic control of food intake and body weight in male rats. Castration of adult male rats produces a delayed (by approximately 1 mo), permanent hypophagia and reduction in weight gain. This contrasts with the rapid, transient hyperphagia and increased weight gain caused by ovariectomy in female rats. Injections of testosterone propionate (TP) stimulate food intake and weight gain in castrated males. Neither 5alpha-reduction nor aromatization of the testosterone molecule plays an important role in the stimulation of these measures by TP. The 5alpha-reduced metabolite of TP, 5alpha-dihydrotestosterone propionate (DHTP), is less effective in increasing eating and weight gain than is TP. Very high doses of TP may actually reduce weight gain with prolonged treatment (2-6 wk). It is likely that this reduced weight gain is due to aromatization of TP to an estrogen. The nonaromatizable androgen, DHTP, does not reduce weight gain even in very high doses, and concurrent progesterone injections reverse the weight-reducing actions of high TP doses.", "PMID": 965512}
{"id": "pubmed23n0032_6394", "title": "Posthatch age and developmental age as a baseline for determination of the sensitive period for imprinting.", "content": "Two experiments comparing developmental age and posthatch age were performed. In the first experiment 143 Peking ducklings of different posthatch ages were imprinted, and their following responses examined in a simultaneous choice test. A sensitive period was evident on the basis of posthatch age. In the second experiment 99 ducklings were separated in three clusters of different posthatch ages, which did not vary within the clusters relative to their probability to be imprinted. The ducklings of each cluster were imprinted at various developmental ages, and the results show the involvement of developmental age. Thus, both experiments considered together favor the concept of an interaction of developmental and posthatch age. Imprinting and the determination of the sensitive period are dependent on prehatch and posthatch learning, so both developmental age and posthatch age are involved in setting the limit of the sensitive period.", "contents": "Posthatch age and developmental age as a baseline for determination of the sensitive period for imprinting. Two experiments comparing developmental age and posthatch age were performed. In the first experiment 143 Peking ducklings of different posthatch ages were imprinted, and their following responses examined in a simultaneous choice test. A sensitive period was evident on the basis of posthatch age. In the second experiment 99 ducklings were separated in three clusters of different posthatch ages, which did not vary within the clusters relative to their probability to be imprinted. The ducklings of each cluster were imprinted at various developmental ages, and the results show the involvement of developmental age. Thus, both experiments considered together favor the concept of an interaction of developmental and posthatch age. Imprinting and the determination of the sensitive period are dependent on prehatch and posthatch learning, so both developmental age and posthatch age are involved in setting the limit of the sensitive period.", "PMID": 965513}
{"id": "pubmed23n0032_6395", "title": "Developing visual function in the red jungle fowl embryo.", "content": "The onset of visual system function in the wild red jungle fowl embryo was measured by the pupillary reflex technique and compared with its domesticated descendant, the domestic chick. The first neurally mediated pupillary reflex in the jungle fowl embryos was found at Day 15 of incubation after 77% of incubation was completed. This point did not differ significantly from the onset of this reflex in the domestic chick embryo (after approximately 83% of incubation). Thus, it was concluded that the relatively late onset of overt visual system function in the chick, as compared with several other precocial avian species, was not a result of its history of intense domestication but rather was most likely a normal characteristic of this and other closely related species.", "contents": "Developing visual function in the red jungle fowl embryo. The onset of visual system function in the wild red jungle fowl embryo was measured by the pupillary reflex technique and compared with its domesticated descendant, the domestic chick. The first neurally mediated pupillary reflex in the jungle fowl embryos was found at Day 15 of incubation after 77% of incubation was completed. This point did not differ significantly from the onset of this reflex in the domestic chick embryo (after approximately 83% of incubation). Thus, it was concluded that the relatively late onset of overt visual system function in the chick, as compared with several other precocial avian species, was not a result of its history of intense domestication but rather was most likely a normal characteristic of this and other closely related species.", "PMID": 965514}
{"id": "pubmed23n0032_6396", "title": "Some effects of conditioned aversion on food intake and olfactory bulb electrical responses in the rat.", "content": "Rats maintained on an unadulterated synthetic food, available from 8 a.m. to 10 a.m. everyday, were submitted to an aversive conditioning schedule on which a first ingestion of eucalyptol-flavored food (EF) was followed by an apomorphine injection (20 mg/kg, ip). In the first experiment the daily food intake was measured from Day 1 to 17, during the first and second hours of the meal. The EF was offered on Days 8, 9, and 17 during the first or the second hour of the meal (Series B or A). On Day 8, the meal was followed in a group of rats by the apomorphine injection. As compared with the intake of Day 8, the mean EF intake of Day 9 was significantly decreased in Series A and B, and of Day 17 in Series A only. No significant EF-intake modification could be observed in a saline-injected group or in an untreated control group. In the second experiment, rats bearing bulbar electrodes for the chronic recording of multiunit mitral cell responses received a 2-hr EF meal before the apomorphine injection. They were stimulated by puffs of odors of pure eucalyptol, unadulterated food, and EF and recorded in hungry and satiated states. Before the aversive conditioning, a significantly greater occurrence of positive responses to the odors of unadulterated food and EF was observed in hungry rats compared with satiated rats. The eucalyptol odor yielded equivalent patterns of responses in hungry and satiated rats before and after conditioning. Conditioning did not alter the modulated responses to unadulterated food odor (a greater occurrence of positive responses was still observed in hungry rats) but modified the responses to the odor of EF (the same high rate of positive responses was then observed in satiated and hungry rats). Electrophysiological data are discussed in terms of palatability changes and food-odor meaning.", "contents": "Some effects of conditioned aversion on food intake and olfactory bulb electrical responses in the rat. Rats maintained on an unadulterated synthetic food, available from 8 a.m. to 10 a.m. everyday, were submitted to an aversive conditioning schedule on which a first ingestion of eucalyptol-flavored food (EF) was followed by an apomorphine injection (20 mg/kg, ip). In the first experiment the daily food intake was measured from Day 1 to 17, during the first and second hours of the meal. The EF was offered on Days 8, 9, and 17 during the first or the second hour of the meal (Series B or A). On Day 8, the meal was followed in a group of rats by the apomorphine injection. As compared with the intake of Day 8, the mean EF intake of Day 9 was significantly decreased in Series A and B, and of Day 17 in Series A only. No significant EF-intake modification could be observed in a saline-injected group or in an untreated control group. In the second experiment, rats bearing bulbar electrodes for the chronic recording of multiunit mitral cell responses received a 2-hr EF meal before the apomorphine injection. They were stimulated by puffs of odors of pure eucalyptol, unadulterated food, and EF and recorded in hungry and satiated states. Before the aversive conditioning, a significantly greater occurrence of positive responses to the odors of unadulterated food and EF was observed in hungry rats compared with satiated rats. The eucalyptol odor yielded equivalent patterns of responses in hungry and satiated rats before and after conditioning. Conditioning did not alter the modulated responses to unadulterated food odor (a greater occurrence of positive responses was still observed in hungry rats) but modified the responses to the odor of EF (the same high rate of positive responses was then observed in satiated and hungry rats). Electrophysiological data are discussed in terms of palatability changes and food-odor meaning.", "PMID": 965515}
{"id": "pubmed23n0032_6397", "title": "Electrical activity of the dorsal hippocampus in rats operantly trained to lever press and to lick.", "content": "One group of four rats was operantly conditioned in a Sidman avoidance procedure to lever press during one discriminative stimulus and to lick during another discriminative stimulus. Another group of four rats was conditioned in a similar manner in order to receive positive reinforcements. More dorsal hippocampal rhythmic slow activity (RSA) occurred during lever pressing than during operant licking. It was also found that dorsal hippocampal RSA accompanied walking and that dorsal hippocampal large irregular activity occurred during periods of immobility, during normal drinking and grooming and during the intense saliva spreading and grooming that were induced by exposure to a 40 degrees C environmental temperature. The data suggested that hippocampal RSA is related to type of response rather than to sensory, motivational, or learning processes. Furthermore, the data suggested that RSA is not related to the operant role of response or to relative vigor of response.", "contents": "Electrical activity of the dorsal hippocampus in rats operantly trained to lever press and to lick. One group of four rats was operantly conditioned in a Sidman avoidance procedure to lever press during one discriminative stimulus and to lick during another discriminative stimulus. Another group of four rats was conditioned in a similar manner in order to receive positive reinforcements. More dorsal hippocampal rhythmic slow activity (RSA) occurred during lever pressing than during operant licking. It was also found that dorsal hippocampal RSA accompanied walking and that dorsal hippocampal large irregular activity occurred during periods of immobility, during normal drinking and grooming and during the intense saliva spreading and grooming that were induced by exposure to a 40 degrees C environmental temperature. The data suggested that hippocampal RSA is related to type of response rather than to sensory, motivational, or learning processes. Furthermore, the data suggested that RSA is not related to the operant role of response or to relative vigor of response.", "PMID": 965516}
{"id": "pubmed23n0032_6398", "title": "Effects of analgesic midbrain stimulation on reflex withdrawal and thermal escape in the rat.", "content": "Strong analgesia produced by mesencephalic electrical brain stimulation in rats significantly increased escape latencies in two-way escape from a floor heated by hydraulic circulation. Reflective reactions to pinching and needling were abolished or greatly diminished. Individual differences in the strengths of analgesia, as assessed by instrumental and reflex indicants, were highly correlated. Induction of analgesia was demonstrated in the absence of positive reinforcement effects produced by brain stimulation. The findings are discussed in relation to possible neural pain suppression systems with critical components situated in the midbrain.", "contents": "Effects of analgesic midbrain stimulation on reflex withdrawal and thermal escape in the rat. Strong analgesia produced by mesencephalic electrical brain stimulation in rats significantly increased escape latencies in two-way escape from a floor heated by hydraulic circulation. Reflective reactions to pinching and needling were abolished or greatly diminished. Individual differences in the strengths of analgesia, as assessed by instrumental and reflex indicants, were highly correlated. Induction of analgesia was demonstrated in the absence of positive reinforcement effects produced by brain stimulation. The findings are discussed in relation to possible neural pain suppression systems with critical components situated in the midbrain.", "PMID": 965517}
{"id": "pubmed23n0032_6399", "title": "Sensory responsiveness and avoidance learning in rats.", "content": "Analysis of sensory responsiveness and avoidance learning was carried out in three experiments using five rat strains. There were significant differences among the strains in response to electric footshock. Also the strain-specific shock intensity as unconditioned stimulus (US) elicited significantly higher rates of avoidance learning as compared with the rates of avoidance learning under an equal but average level of shock intensity as US. In general, discrete auditory and visual sensory modes as conditioned stimuli (CS) produced almost the same rate of avoidance learning. The proportion of variation in avoidance learning attributable to strains was significant under all six experimental conditions except no-discrete CS and strain-specific US condition.", "contents": "Sensory responsiveness and avoidance learning in rats. Analysis of sensory responsiveness and avoidance learning was carried out in three experiments using five rat strains. There were significant differences among the strains in response to electric footshock. Also the strain-specific shock intensity as unconditioned stimulus (US) elicited significantly higher rates of avoidance learning as compared with the rates of avoidance learning under an equal but average level of shock intensity as US. In general, discrete auditory and visual sensory modes as conditioned stimuli (CS) produced almost the same rate of avoidance learning. The proportion of variation in avoidance learning attributable to strains was significant under all six experimental conditions except no-discrete CS and strain-specific US condition.", "PMID": 965518}
{"id": "pubmed23n0032_6400", "title": "Trial development of unit response changes during conditioning in cats.", "content": "Single-cell and evoked potential (EP) activity in the postcruciate cortex of acutely prepared cats was recorded during a classical conditioning procedure. The temporal pattern of response (PSH) of the neuron was separated into three components, and the trial development of learning changes for these components was examined individually. Changes over trials in the similarity of the EP waveform and the unit PSH were also examined. The unit response components showed changes in response at different trial periods; these changes could not be accounted for by changes at lower brain levels that projected to the cortex. This suggests that local learning changes were taking places in the cortex. The EP-PSH similarity decreased for neurons showing the largest changes in response during conditioning. Since the EP reflects the response of the whole population of neurons and a majority did not show learning changes, this could account for the observed decrease.", "contents": "Trial development of unit response changes during conditioning in cats. Single-cell and evoked potential (EP) activity in the postcruciate cortex of acutely prepared cats was recorded during a classical conditioning procedure. The temporal pattern of response (PSH) of the neuron was separated into three components, and the trial development of learning changes for these components was examined individually. Changes over trials in the similarity of the EP waveform and the unit PSH were also examined. The unit response components showed changes in response at different trial periods; these changes could not be accounted for by changes at lower brain levels that projected to the cortex. This suggests that local learning changes were taking places in the cortex. The EP-PSH similarity decreased for neurons showing the largest changes in response during conditioning. Since the EP reflects the response of the whole population of neurons and a majority did not show learning changes, this could account for the observed decrease.", "PMID": 965519}
{"id": "pubmed23n0032_6401", "title": "Dissociation of gustatory and weight regulatory responses to quinine following lateral hypothalamic lesions.", "content": "In a two-phase experiment, consummatory (Phase 1) and body weight regulation (Phase 2) responses to quinine adulteration of a wet mash diet were measured in rats recovered from bilateral lateral hypothalamic lesions (LH; n = 16) and in unoperated control rats (C; n = 18). In Phase 1, all rats were fed wet mash adulterated with increasing concentrations of quinine sulfate every other day, and fed unadulterated wet mash on the alternate days. Group LH consumed a significantly lower proportion of quinine-adulterated wet mash relative to unadulterated wet mash, displaying a steeper concentration-response function and a lower rejection threshold than did Group C. In Phase 2, Groups LH and C were maintained exclusively on quinine-adulterated mash for 20 days. This procedure caused equivalent weight loss in the two groups. Therefore, an apparent exaggerated aversion to quinine-adulterated food does not appear to contribute abnormally to the weight regulation exhibited by rats with lateral hypothalamic damage.", "contents": "Dissociation of gustatory and weight regulatory responses to quinine following lateral hypothalamic lesions. In a two-phase experiment, consummatory (Phase 1) and body weight regulation (Phase 2) responses to quinine adulteration of a wet mash diet were measured in rats recovered from bilateral lateral hypothalamic lesions (LH; n = 16) and in unoperated control rats (C; n = 18). In Phase 1, all rats were fed wet mash adulterated with increasing concentrations of quinine sulfate every other day, and fed unadulterated wet mash on the alternate days. Group LH consumed a significantly lower proportion of quinine-adulterated wet mash relative to unadulterated wet mash, displaying a steeper concentration-response function and a lower rejection threshold than did Group C. In Phase 2, Groups LH and C were maintained exclusively on quinine-adulterated mash for 20 days. This procedure caused equivalent weight loss in the two groups. Therefore, an apparent exaggerated aversion to quinine-adulterated food does not appear to contribute abnormally to the weight regulation exhibited by rats with lateral hypothalamic damage.", "PMID": 965520}
{"id": "pubmed23n0032_6402", "title": "Marginal and extramarginal cortical lesions and visual discrimination by cats.", "content": "Seventy-five cats learned a shape discrimination with zero, one, or two irrelevant cues. They were then subjected to sham operations (n = 34), ablation of the marginal and splenial gyri (n = 9), or lesions in the extramarginal (EM) cortex. The 32 EM cats comprised four groups, three with small (EM1, n = 9), intermediate (EM2, n = 10), and large (EM3, n = 9) decortications, and a fourth group with both EM lesions and heavy degeneration in the lateral geniculate nucleus (LGN, n = 4). The cats with marginal or extensive extramarginal lesions were severely impaired in shape and size discrimination. Two lines of evidence indicate that their behavioral defects resulted from different neural dysfunctions. (a) The errors made by marginal gyrus cases increased sharply as a function of the number of irrelevant cues present in shape discrimination training; no other group, including Group EM3, was affected by this variable. (b) Cats with extramarginal ablations and strong LGN degeneration were no more severely impaired than were subjects with comparable extramarginal damage and little or no LGN degeneration. While the nature of the two kinds of deficits remains unclear, they seem parallel to those following posterior cortical lesions in monkeys.", "contents": "Marginal and extramarginal cortical lesions and visual discrimination by cats. Seventy-five cats learned a shape discrimination with zero, one, or two irrelevant cues. They were then subjected to sham operations (n = 34), ablation of the marginal and splenial gyri (n = 9), or lesions in the extramarginal (EM) cortex. The 32 EM cats comprised four groups, three with small (EM1, n = 9), intermediate (EM2, n = 10), and large (EM3, n = 9) decortications, and a fourth group with both EM lesions and heavy degeneration in the lateral geniculate nucleus (LGN, n = 4). The cats with marginal or extensive extramarginal lesions were severely impaired in shape and size discrimination. Two lines of evidence indicate that their behavioral defects resulted from different neural dysfunctions. (a) The errors made by marginal gyrus cases increased sharply as a function of the number of irrelevant cues present in shape discrimination training; no other group, including Group EM3, was affected by this variable. (b) Cats with extramarginal ablations and strong LGN degeneration were no more severely impaired than were subjects with comparable extramarginal damage and little or no LGN degeneration. While the nature of the two kinds of deficits remains unclear, they seem parallel to those following posterior cortical lesions in monkeys.", "PMID": 965521}
{"id": "pubmed23n0032_6403", "title": "Performance on the visual cliff by cats with marginal gyrus lesions.", "content": "Lesions in 12 cats that destroyed almost all of area 17 (Group MS) abolished differential responding on the visual cliff. Performance was not impaired in 9 cats in which regions of the striate cortex were spared in the depths of the splenial sulcus or in 23 cats with damage to extramarginal areas. There were 37 controls. Additional experiments indicated that the deficit in performance by MS cats was not reduced either by the administration of amphetamine or by increases in cues for motion parallax. Monocularly occluded normal cats preferred the shallow surface of the visual cliff, demonstrating that the deficit was not due solely to removal of neurons sensitive to binocular disparity. The findings were discussed in light of electrophysiological evidence that lesions of the visual cortex disrupt functions of the superior colliculus.", "contents": "Performance on the visual cliff by cats with marginal gyrus lesions. Lesions in 12 cats that destroyed almost all of area 17 (Group MS) abolished differential responding on the visual cliff. Performance was not impaired in 9 cats in which regions of the striate cortex were spared in the depths of the splenial sulcus or in 23 cats with damage to extramarginal areas. There were 37 controls. Additional experiments indicated that the deficit in performance by MS cats was not reduced either by the administration of amphetamine or by increases in cues for motion parallax. Monocularly occluded normal cats preferred the shallow surface of the visual cliff, demonstrating that the deficit was not due solely to removal of neurons sensitive to binocular disparity. The findings were discussed in light of electrophysiological evidence that lesions of the visual cortex disrupt functions of the superior colliculus.", "PMID": 965522}
{"id": "pubmed23n0032_6404", "title": "Sex differences in sensitivity of food intake, body weight, and running-wheel activity to ovarian steroids in rats.", "content": "The effects of sex and experimental androgenization on the responsiveness of food intake and running-wheel activity to exogenously administered ovarian hormones were investigated in adult, gonadectomized rats. Food intake was found to be analogous to lordotic behavior in that males and neonatally androgenized females were relatively insensitive to the influences of either estradiol benzoate (EB) or progesterone (P). Running-wheel activity, however, could be differentiated from food intake in that males responded the same as females to the effects of EB and P. Androgenized females (500-mug testosterone propionate on the third day of life) showed a response to EB quantitatively equivalent to that of nonandrogenized females, but they had a longer latency to respond. The estrogen-antagonistic effects of P were confirmed for both of these behaviors, and there was a positive correlation between the magnitude of the responses to EB and P.", "contents": "Sex differences in sensitivity of food intake, body weight, and running-wheel activity to ovarian steroids in rats. The effects of sex and experimental androgenization on the responsiveness of food intake and running-wheel activity to exogenously administered ovarian hormones were investigated in adult, gonadectomized rats. Food intake was found to be analogous to lordotic behavior in that males and neonatally androgenized females were relatively insensitive to the influences of either estradiol benzoate (EB) or progesterone (P). Running-wheel activity, however, could be differentiated from food intake in that males responded the same as females to the effects of EB and P. Androgenized females (500-mug testosterone propionate on the third day of life) showed a response to EB quantitatively equivalent to that of nonandrogenized females, but they had a longer latency to respond. The estrogen-antagonistic effects of P were confirmed for both of these behaviors, and there was a positive correlation between the magnitude of the responses to EB and P.", "PMID": 965523}
{"id": "pubmed23n0032_6405", "title": "Selection for spontaneous or priming-induced audiogenic seizure susceptibility in mice.", "content": "Four lines of mice were selectively bred from a heterogeneous foundation stock for audiogenic seizure prone (SP), priming prone (PP), moderately priming prone (MPP), and seizure resistant (SR). Significant changes in proportions of animals showing the desired phenotypes were found after two generations of selection, indicating involvement of genetic components in these behavioral characteristics. Although response to selection for spontaneous seizure proneness was rapid, the results do not support a view that initial seizure risk is controlled by a single recessive gene. The effects of tympanic membrane perforation on development of seizure susceptibility in these four selected lines were investigated in Experiment 2. Results indicate that the method is highly effective in inducing seizure susceptibility in the PP mice and the SR mice, but not so effective for the SP and the MPP lines. These results suggest that spontaneous and priming-induced seizure susceptibility could be due to development of hyperreactivity in centripetal auditory structures brought about by reduction of auditory input. They also suggest that the phenotypic difference between the PP and the SR lines could be due to differences in their cochlear susceptibility to stimulation damage but that a qualitatively different mechanism is involved in the MPP line.", "contents": "Selection for spontaneous or priming-induced audiogenic seizure susceptibility in mice. Four lines of mice were selectively bred from a heterogeneous foundation stock for audiogenic seizure prone (SP), priming prone (PP), moderately priming prone (MPP), and seizure resistant (SR). Significant changes in proportions of animals showing the desired phenotypes were found after two generations of selection, indicating involvement of genetic components in these behavioral characteristics. Although response to selection for spontaneous seizure proneness was rapid, the results do not support a view that initial seizure risk is controlled by a single recessive gene. The effects of tympanic membrane perforation on development of seizure susceptibility in these four selected lines were investigated in Experiment 2. Results indicate that the method is highly effective in inducing seizure susceptibility in the PP mice and the SR mice, but not so effective for the SP and the MPP lines. These results suggest that spontaneous and priming-induced seizure susceptibility could be due to development of hyperreactivity in centripetal auditory structures brought about by reduction of auditory input. They also suggest that the phenotypic difference between the PP and the SR lines could be due to differences in their cochlear susceptibility to stimulation damage but that a qualitatively different mechanism is involved in the MPP line.", "PMID": 965524}
{"id": "pubmed23n0032_6406", "title": "Inhibition of acoustic priming in mice.", "content": "Mice of the C57BL/6J strain can be made susceptible to audiogenic seizures by a process known as acoustic priming. Acoustic priming can be blocked when the animals are injected either with puromycin or with puromycin aminonucleoside before the application of the priming stimulus. Cycloheximide, diphenylhydantoin, and d-amphetamine had little effect on priming-induced audiogenic seizures in these animals. All of these drugs, however, when given in combination with puromycin reversed in the protective action of puromycin against audiogenic seizures. Puromycin administered to 19-day-old mice increased susceptibility to electroconvulsive seizures when the animals were tested at 22 days of age. It is suggested that puromycin is able to block priming-induced audiogenic seizures by producing abnormal electrical activity in the brain or through an interference with normal neurohumoral transmission by incomplete peptides.", "contents": "Inhibition of acoustic priming in mice. Mice of the C57BL/6J strain can be made susceptible to audiogenic seizures by a process known as acoustic priming. Acoustic priming can be blocked when the animals are injected either with puromycin or with puromycin aminonucleoside before the application of the priming stimulus. Cycloheximide, diphenylhydantoin, and d-amphetamine had little effect on priming-induced audiogenic seizures in these animals. All of these drugs, however, when given in combination with puromycin reversed in the protective action of puromycin against audiogenic seizures. Puromycin administered to 19-day-old mice increased susceptibility to electroconvulsive seizures when the animals were tested at 22 days of age. It is suggested that puromycin is able to block priming-induced audiogenic seizures by producing abnormal electrical activity in the brain or through an interference with normal neurohumoral transmission by incomplete peptides.", "PMID": 965525}
{"id": "pubmed23n0032_6407", "title": "DRL performance, extinction, and secondary reinforcement: role of appetitive value of food in mice with septal lesions.", "content": "Normal mice and mice with septal lesions were trained on a differential-reinforcement-for-low-rates-of-responding (DRL 8-sec) schedule for the reinforcement varying in incentive value. Dilution of diet increased the number of reinforcements received by mice with septal lesions. In Experiments 2 and 3 the effects of septal lesions on resistance to extinction and continuous reinforcement training and the strength of secondary reinforcement were investigated. Changes in reinforcement value modified the septal lesion effects in both cases. All three experiments demonstrated the alteration of the septal lesion effect through a change in the appetitive value of the reinforcement. The results suggest that one of the mechanisms by which septal lesions impair DRL performance is an enhancement of reinforcing properties of food.", "contents": "DRL performance, extinction, and secondary reinforcement: role of appetitive value of food in mice with septal lesions. Normal mice and mice with septal lesions were trained on a differential-reinforcement-for-low-rates-of-responding (DRL 8-sec) schedule for the reinforcement varying in incentive value. Dilution of diet increased the number of reinforcements received by mice with septal lesions. In Experiments 2 and 3 the effects of septal lesions on resistance to extinction and continuous reinforcement training and the strength of secondary reinforcement were investigated. Changes in reinforcement value modified the septal lesion effects in both cases. All three experiments demonstrated the alteration of the septal lesion effect through a change in the appetitive value of the reinforcement. The results suggest that one of the mechanisms by which septal lesions impair DRL performance is an enhancement of reinforcing properties of food.", "PMID": 965526}
{"id": "pubmed23n0032_6408", "title": "Grooming in the rat as an aftereffect of lateral hypothalamic stimulation.", "content": "Grooming occurred as an aftereffect of electrical stimulation at hypothalamic sites that elicited locomotion, drinking, or eating as stimulus-bound behaviors. Removal of food or water, which caused rats to switch from one stimulus-bound behavior to another, produced little or no change in the grooming aftereffect. Stimulation with pulse showed that the absolute refractory period for the neurons responsible for the occurrence of grooming is approximately 1 msec. This value is longer than those reported for drinking, eating, and locomotion. Finally, it was found that with some electrodes, low levels of stimulation elicited grooming directly, during the stimulation; at higher levels grooming occurred only as an aftereffect. It is concluded that grooming is activated through neurons separate from those that produce drinking, eating, or locomotion and that its occurrence as an aftereffect may be due to an interaction between short-lasting inhibitory and longer lasting excitatory effects of the stimulation.", "contents": "Grooming in the rat as an aftereffect of lateral hypothalamic stimulation. Grooming occurred as an aftereffect of electrical stimulation at hypothalamic sites that elicited locomotion, drinking, or eating as stimulus-bound behaviors. Removal of food or water, which caused rats to switch from one stimulus-bound behavior to another, produced little or no change in the grooming aftereffect. Stimulation with pulse showed that the absolute refractory period for the neurons responsible for the occurrence of grooming is approximately 1 msec. This value is longer than those reported for drinking, eating, and locomotion. Finally, it was found that with some electrodes, low levels of stimulation elicited grooming directly, during the stimulation; at higher levels grooming occurred only as an aftereffect. It is concluded that grooming is activated through neurons separate from those that produce drinking, eating, or locomotion and that its occurrence as an aftereffect may be due to an interaction between short-lasting inhibitory and longer lasting excitatory effects of the stimulation.", "PMID": 965527}
{"id": "pubmed23n0032_6409", "title": "Drug exposure and the acquisition and retention of a conditioned taste aversion.", "content": "Two experiments examined the effects of preexposure and postexposure to a drug on the acquisition and retention of a conditioned taste aversion induced by that drug. Experiment 1 demonstrated that although drug preexposure attenuated a subsequent conditioned aversion, repeated taste-drug pairings reversed the initial attenuation effect and resulted in nearly complete avoidance of consumption. Experiment 2, however, demonstrated that drug postexposure did not alter a previously established conditioned aversion, although the postexposure experiences were effective in attenuating a conditioned aversion to a second novel solution. It was suggested that conditioned aversions are mediated by ACTH and that preexposure to a drug results in tolerance to that drug, yielding a smaller ACTH response and thereby a weaker aversion.", "contents": "Drug exposure and the acquisition and retention of a conditioned taste aversion. Two experiments examined the effects of preexposure and postexposure to a drug on the acquisition and retention of a conditioned taste aversion induced by that drug. Experiment 1 demonstrated that although drug preexposure attenuated a subsequent conditioned aversion, repeated taste-drug pairings reversed the initial attenuation effect and resulted in nearly complete avoidance of consumption. Experiment 2, however, demonstrated that drug postexposure did not alter a previously established conditioned aversion, although the postexposure experiences were effective in attenuating a conditioned aversion to a second novel solution. It was suggested that conditioned aversions are mediated by ACTH and that preexposure to a drug results in tolerance to that drug, yielding a smaller ACTH response and thereby a weaker aversion.", "PMID": 965528}
{"id": "pubmed23n0032_6410", "title": "Ontogeny of persistence: immediate and long-term persistence in rats varying in training age between 17 and 65 days.", "content": "In Experiment 1, six age groups of rats were trained to run an alley for either partial (PRF) or continuous reinforcement (CRF). Training was during a 2-day period starting at six different ages, and extinction was initiated about 12 hr later. There was a clear partial reinforcement extinction effect (PREE) at all ages and, particularly after PRF training, an inverse relationship between resistance to extinction and age. In Experiment 2, retention and durability of persistence as well as immediate persistence were tested following acquisition at three different ages. The immediate extinction tests confirmed the results of Experiment 1. In the delayed extinction tests, greater resistance to extinction following PRF than following CRF was found in all age groups after both the retention and durability manipulations. In neither test was there an effect of age in original acquisition on the magnitude of adult persistence. These experiments confirm earlier findings of remarkable persistence in rats trained and tested at weanling age, show that the persistence is even greater in pre-weanlings, and show that it is retained into young adulthood.", "contents": "Ontogeny of persistence: immediate and long-term persistence in rats varying in training age between 17 and 65 days. In Experiment 1, six age groups of rats were trained to run an alley for either partial (PRF) or continuous reinforcement (CRF). Training was during a 2-day period starting at six different ages, and extinction was initiated about 12 hr later. There was a clear partial reinforcement extinction effect (PREE) at all ages and, particularly after PRF training, an inverse relationship between resistance to extinction and age. In Experiment 2, retention and durability of persistence as well as immediate persistence were tested following acquisition at three different ages. The immediate extinction tests confirmed the results of Experiment 1. In the delayed extinction tests, greater resistance to extinction following PRF than following CRF was found in all age groups after both the retention and durability manipulations. In neither test was there an effect of age in original acquisition on the magnitude of adult persistence. These experiments confirm earlier findings of remarkable persistence in rats trained and tested at weanling age, show that the persistence is even greater in pre-weanlings, and show that it is retained into young adulthood.", "PMID": 965529}
{"id": "pubmed23n0032_6411", "title": "Olfactory recognition of conspecifics by domestic Norway rats.", "content": "In order to learn more about their ability to recognize one another via olfaction, domestic male rats were given a series of preference tests in which pairs of odors from male conspecifics were presented. Both immature and mature males prefer (p less than .05) the odor from immature strangers over that from immature cage mates but are indifferent to the ordors from mature strangers versus cage mates. Both immature and mature males prefer (p less than .05) the odor from mature novel cage mates over that from mature cage mates to which they are temporarily habituated but are indifferent to the odors from immature novel versus familial cage mates. Mature males prefer (p less than .08) the odor from a cage mate over the subject's own odor, and they prefer (p less than .01) their own odor over no odor. Under certain conditions, male rats can discriminate between the odors from (a) strangers versus cage mates, (b) two cage mates, and (c) their own body versus a cage mate.", "contents": "Olfactory recognition of conspecifics by domestic Norway rats. In order to learn more about their ability to recognize one another via olfaction, domestic male rats were given a series of preference tests in which pairs of odors from male conspecifics were presented. Both immature and mature males prefer (p less than .05) the odor from immature strangers over that from immature cage mates but are indifferent to the ordors from mature strangers versus cage mates. Both immature and mature males prefer (p less than .05) the odor from mature novel cage mates over that from mature cage mates to which they are temporarily habituated but are indifferent to the odors from immature novel versus familial cage mates. Mature males prefer (p less than .08) the odor from a cage mate over the subject's own odor, and they prefer (p less than .01) their own odor over no odor. Under certain conditions, male rats can discriminate between the odors from (a) strangers versus cage mates, (b) two cage mates, and (c) their own body versus a cage mate.", "PMID": 965530}
{"id": "pubmed23n0032_6412", "title": "Olfactory denervation: its biological and behavioral effects in infant rats.", "content": "Two-week-old infant rats were treated with intranasal ZnSO4 and compared with littlemate controls 24 hr later. When infants were returned to their mother and littermates, those with olfactory deficits lost weight, had reduced cardiac and respiratory rates and lower body temperatures, and showed increased locomotor behavior in an unfamiliar test area. Subsequent experiments showed that in the absence of the mother and also in isolation, differences between ZnSO4-treated and control infants involved fewer systems were less marked, and presented different patterns. Isolated infants given oral ZnSO4 did not differ from controls on any measures. Olfactory denervation appears to produce these effects by disrupting nursing, by altering littermate interaction, and by other central nervous system effects that are independent of social interaction.", "contents": "Olfactory denervation: its biological and behavioral effects in infant rats. Two-week-old infant rats were treated with intranasal ZnSO4 and compared with littlemate controls 24 hr later. When infants were returned to their mother and littermates, those with olfactory deficits lost weight, had reduced cardiac and respiratory rates and lower body temperatures, and showed increased locomotor behavior in an unfamiliar test area. Subsequent experiments showed that in the absence of the mother and also in isolation, differences between ZnSO4-treated and control infants involved fewer systems were less marked, and presented different patterns. Isolated infants given oral ZnSO4 did not differ from controls on any measures. Olfactory denervation appears to produce these effects by disrupting nursing, by altering littermate interaction, and by other central nervous system effects that are independent of social interaction.", "PMID": 965531}
{"id": "pubmed23n0032_6413", "title": "Hormonal influences on sexual dimorphism in rate of extinction of a conditioned taste aversion in rats.", "content": "The hormonal influences on the slow extinction rate of a conditioned taste aversion shown by male rats and the fast extinction rate shown by female rats were investigated. When males were castrated, they extinguished as quickly as females. When castrated males were given testosterone propionate replacement, they had a slow extinction rate. Castration had no effect on the extinction rate of females. But when testosterone propionate was administered to castrated or intact females, they had a slow, malelike extinction rate. Thus, sexual dimorphism in the extinction rate of a conditioned taste aversion seems to be due to the activational effects of testosterone.", "contents": "Hormonal influences on sexual dimorphism in rate of extinction of a conditioned taste aversion in rats. The hormonal influences on the slow extinction rate of a conditioned taste aversion shown by male rats and the fast extinction rate shown by female rats were investigated. When males were castrated, they extinguished as quickly as females. When castrated males were given testosterone propionate replacement, they had a slow extinction rate. Castration had no effect on the extinction rate of females. But when testosterone propionate was administered to castrated or intact females, they had a slow, malelike extinction rate. Thus, sexual dimorphism in the extinction rate of a conditioned taste aversion seems to be due to the activational effects of testosterone.", "PMID": 965532}
{"id": "pubmed23n0032_6414", "title": "Failure to show modification of male golden hamster mating behavior through taste/odor aversion learning.", "content": "This research investigated whether the dependence of mating behavior in the male hamster on olfactory and gustatory stimuli could be used with the taste/odor conditioning paradigm to alter sexual behavior. Preliminary experiments established the effectiveness of lithium chloride and methyl-atropine nitrate in forming aversions to a saccharin solution. These same agents failed to modify mating behavior when the conditional stimulus was an estrous female hamster. Animals made ill after an exposure to a cotton swab containing phenylacetic acid later showed increased latencies in the initiation of mating with an estrous female swabbed with phenylacetic acid. Animals given three punished exposures to phenylacetic acid also showed increased latency to initiate mating and decreased anogenital sniff-licking with a phenylacetic acid-swabbed estrous female. However, no other measures of mating behavior were altered.", "contents": "Failure to show modification of male golden hamster mating behavior through taste/odor aversion learning. This research investigated whether the dependence of mating behavior in the male hamster on olfactory and gustatory stimuli could be used with the taste/odor conditioning paradigm to alter sexual behavior. Preliminary experiments established the effectiveness of lithium chloride and methyl-atropine nitrate in forming aversions to a saccharin solution. These same agents failed to modify mating behavior when the conditional stimulus was an estrous female hamster. Animals made ill after an exposure to a cotton swab containing phenylacetic acid later showed increased latencies in the initiation of mating with an estrous female swabbed with phenylacetic acid. Animals given three punished exposures to phenylacetic acid also showed increased latency to initiate mating and decreased anogenital sniff-licking with a phenylacetic acid-swabbed estrous female. However, no other measures of mating behavior were altered.", "PMID": 965533}
{"id": "pubmed23n0032_6415", "title": "Habituation and tonic immobility in domestic chickens.", "content": "The habituation of tonic immobility in chickens was examined in six studies. It was shown that repeated elicitation of immobility, and not just handling, was responsible for reduced response durations after multiple exposures to manual restraint. Habituation was a function of the number of stimulus presentations and, in addition, proved surprisingly durable, with diminished reactions using lasting at least 2.5 mo. Strain differences were found in the number of trials required to reach a criteria of habituation, and habituation proceeded faster when immobility termination was self-paced as opposed to experimenter induced. Also, massed trials produced robust sensitization effects rather than diminished responsiveness.", "contents": "Habituation and tonic immobility in domestic chickens. The habituation of tonic immobility in chickens was examined in six studies. It was shown that repeated elicitation of immobility, and not just handling, was responsible for reduced response durations after multiple exposures to manual restraint. Habituation was a function of the number of stimulus presentations and, in addition, proved surprisingly durable, with diminished reactions using lasting at least 2.5 mo. Strain differences were found in the number of trials required to reach a criteria of habituation, and habituation proceeded faster when immobility termination was self-paced as opposed to experimenter induced. Also, massed trials produced robust sensitization effects rather than diminished responsiveness.", "PMID": 965534}
{"id": "pubmed23n0032_6416", "title": "Spontaneous and centrally induced behaviors in normal and thalamic opossums.", "content": "Electrical stimulation of localized areas of the opossum medulla and cerebellum induced goal-oriented eating and grooming behaviors of stereotyped gagging. Following radical telencephalic lesions, these animals showed considerable behavioral capacity and in many cases continued to demonstrate centrally induced responses. Animals with lesions, however, failed to groom or show active food-seeking behavior, and they showed poor thermoregulation in the heat, presumably because of the loss of thermoregulatory grooming. These deficits following removal of the telencephalon appear to have resulted from disruption of important sources of control over behaviors rather than from loss of basic consummatory response mechanisms, since grooming and eating, while less directed, could still be elicited by central stimulation. These results support the view that the lower brain stem may contain intrinsic neural circuits for the integration of complex behaviors.", "contents": "Spontaneous and centrally induced behaviors in normal and thalamic opossums. Electrical stimulation of localized areas of the opossum medulla and cerebellum induced goal-oriented eating and grooming behaviors of stereotyped gagging. Following radical telencephalic lesions, these animals showed considerable behavioral capacity and in many cases continued to demonstrate centrally induced responses. Animals with lesions, however, failed to groom or show active food-seeking behavior, and they showed poor thermoregulation in the heat, presumably because of the loss of thermoregulatory grooming. These deficits following removal of the telencephalon appear to have resulted from disruption of important sources of control over behaviors rather than from loss of basic consummatory response mechanisms, since grooming and eating, while less directed, could still be elicited by central stimulation. These results support the view that the lower brain stem may contain intrinsic neural circuits for the integration of complex behaviors.", "PMID": 965535}
{"id": "pubmed23n0032_6417", "title": "Oropharyngeal control of drinking in rats.", "content": "Drinking was studied in water-deprived rats with chronic intragastric fistulas. Sham drinking, by which the ingested water was immediately lost through the open gastric fistula, was approximately four times greater than normal drinking. This proportion held across different levels of water deprivation, following 5-ml intragastric preloads of water or isotonic saline or ingestion of isotonic saline. The contribution of oropharyngeal controls in the normal drinking of rats is discussed and is compared with that of other species.", "contents": "Oropharyngeal control of drinking in rats. Drinking was studied in water-deprived rats with chronic intragastric fistulas. Sham drinking, by which the ingested water was immediately lost through the open gastric fistula, was approximately four times greater than normal drinking. This proportion held across different levels of water deprivation, following 5-ml intragastric preloads of water or isotonic saline or ingestion of isotonic saline. The contribution of oropharyngeal controls in the normal drinking of rats is discussed and is compared with that of other species.", "PMID": 965536}
{"id": "pubmed23n0032_6418", "title": "Effect of high dietary zinc upon zinc metabolism and intracellular distribution in cows and calves.", "content": "Changes in the metabolism of zinc were investigated in mature nonlactating Holstein cows and 4-mo old calves fed high but nontoxic amounts of dietary zinc. Increasing dietary zinc of calves from 42 to 642 ppm elevated the zinc content of liver and pancreas 600% and 1400%, respectively. The elevated zinc in liver and pancreas involved a substantial increase in all intracellular fractions, with by far the largest amount associated with a 10,000 molecular-weight protein in the soluble cell fraction. This zinc-binding protein may protect the cell against damage from increased zinc. A comparable increase in dietary zinc of mature cows did not affect zinc content of liver or kidney nor alter the intracellular distribution of zinc in the liver. In mature cows, homeostatic control mechanisms which regulate the zinc content of tissue are much more effective than in calves. Accordingly, mature cows probably are able to tolerate higher concentrations of dietary zinc.", "contents": "Effect of high dietary zinc upon zinc metabolism and intracellular distribution in cows and calves. Changes in the metabolism of zinc were investigated in mature nonlactating Holstein cows and 4-mo old calves fed high but nontoxic amounts of dietary zinc. Increasing dietary zinc of calves from 42 to 642 ppm elevated the zinc content of liver and pancreas 600% and 1400%, respectively. The elevated zinc in liver and pancreas involved a substantial increase in all intracellular fractions, with by far the largest amount associated with a 10,000 molecular-weight protein in the soluble cell fraction. This zinc-binding protein may protect the cell against damage from increased zinc. A comparable increase in dietary zinc of mature cows did not affect zinc content of liver or kidney nor alter the intracellular distribution of zinc in the liver. In mature cows, homeostatic control mechanisms which regulate the zinc content of tissue are much more effective than in calves. Accordingly, mature cows probably are able to tolerate higher concentrations of dietary zinc.", "PMID": 965537}
{"id": "pubmed23n0032_6419", "title": "Fertility of frozen bovine spermatozoa packaged in continental straws or ampules.", "content": "Fertility of semen frozen in .25-ml Continental straws or 1.0-ml ampules was compared in two field trials. Semen from four bulls was used to inseminate 574 cows over 38 days in Trial 1. Rates of pregnancy for cows inseminated with semen in straws or ampules were 54 and 32% for first-service inseminations and 70 and 50% at the end of the breeding period. Semen from one bull was used to inseminate 204 cows over 44 days in Trial 2. Rates of pregnancy from single inseminations averaged 58 and 44%, while corresponding values at the end of the breeding period were 83 and 67% for semen in straws and ampules.", "contents": "Fertility of frozen bovine spermatozoa packaged in continental straws or ampules. Fertility of semen frozen in .25-ml Continental straws or 1.0-ml ampules was compared in two field trials. Semen from four bulls was used to inseminate 574 cows over 38 days in Trial 1. Rates of pregnancy for cows inseminated with semen in straws or ampules were 54 and 32% for first-service inseminations and 70 and 50% at the end of the breeding period. Semen from one bull was used to inseminate 204 cows over 44 days in Trial 2. Rates of pregnancy from single inseminations averaged 58 and 44%, while corresponding values at the end of the breeding period were 83 and 67% for semen in straws and ampules.", "PMID": 965538}
{"id": "pubmed23n0032_6420", "title": "Effect of glucagon infusion on plasma magnesium, glucose, and insulin in bull calves.", "content": "Two Holstein bull calves each were infused intravenously with 1 mg glucagon in .9% sodium chloride, and two were given saline alone; 1 wk later treatments were reversed. Glucagon increased concentrations of insulin and glucose but decreased potassium in blood plasma and moderately increased urinary magnesium and calcium losses. When only saline was used, there was no effect. A hypothesis relating elevated glucagon to grass tetany is proposed.", "contents": "Effect of glucagon infusion on plasma magnesium, glucose, and insulin in bull calves. Two Holstein bull calves each were infused intravenously with 1 mg glucagon in .9% sodium chloride, and two were given saline alone; 1 wk later treatments were reversed. Glucagon increased concentrations of insulin and glucose but decreased potassium in blood plasma and moderately increased urinary magnesium and calcium losses. When only saline was used, there was no effect. A hypothesis relating elevated glucagon to grass tetany is proposed.", "PMID": 965539}
{"id": "pubmed23n0032_6421", "title": "Fat cow syndrome.", "content": "The objective of this paper is to characterize the fat cow syndrome. This condition refers to a combination of metabolic, digestive, infectious, and reproductive conditions which affects the obese periparturient cow. The condition develops primarily due to faulty feed management which permits excessive consumption of unbalanced diets. The syndrome is frequently a herd problem characterized by a high morbidity and mortality due to an increase in disease in periparturient cows. Clinical signs include depression, anorexia, ketonuria, marked decrease in production, progressive debilitation, weakness, nervous signs, and an elevation in temperature due to infectious disease. The obesity is generalized throughout the body with extensive fatty metamorphosis in the liver. Histological changes are primarily in the liver and kidney. Treatment of the condition consists of feeding a balanced diet, symptomatic treatment, and good supportive care. The condition can be prevented by feeding a balanced diet according to nutrient requirements of the National Research Council.", "contents": "Fat cow syndrome. The objective of this paper is to characterize the fat cow syndrome. This condition refers to a combination of metabolic, digestive, infectious, and reproductive conditions which affects the obese periparturient cow. The condition develops primarily due to faulty feed management which permits excessive consumption of unbalanced diets. The syndrome is frequently a herd problem characterized by a high morbidity and mortality due to an increase in disease in periparturient cows. Clinical signs include depression, anorexia, ketonuria, marked decrease in production, progressive debilitation, weakness, nervous signs, and an elevation in temperature due to infectious disease. The obesity is generalized throughout the body with extensive fatty metamorphosis in the liver. Histological changes are primarily in the liver and kidney. Treatment of the condition consists of feeding a balanced diet, symptomatic treatment, and good supportive care. The condition can be prevented by feeding a balanced diet according to nutrient requirements of the National Research Council.", "PMID": 965540}
{"id": "pubmed23n0032_6422", "title": "Study on cockroach antigen as a probable causative agent in bronchial asthma.", "content": "Bronchial provocative test (BPT) with cockroach (CR) antigen was performed in 22 asthmatic subjects. Sixteen had positive reaction (CR-positive) and 6 had negative reaction (CR-negative) to CR antigen by skin test. Immediate bronchoconstrictive response was noted following the antigen inhalation in 14 of 16 CR-positive asthmatics, while none of 6 CR-negative asthmatics showed bronchospasm. Late asthmatic responses also were noted in 13 of 16 CR-positive asthmatic individuals following BPT with CR antigen. The dual asthmatic reactions in CR-positive individuals were mostly inhibited by the prior administration of cromolyn sodium. Three-fold increases in peripheral eosinophil counts were noted 24 hr following BPT with CR antigen. Results indicate that CR-induced asthmatic responses are allergen-specific and CR plays a causative role in allergic asthma in the population studied.", "contents": "Study on cockroach antigen as a probable causative agent in bronchial asthma. Bronchial provocative test (BPT) with cockroach (CR) antigen was performed in 22 asthmatic subjects. Sixteen had positive reaction (CR-positive) and 6 had negative reaction (CR-negative) to CR antigen by skin test. Immediate bronchoconstrictive response was noted following the antigen inhalation in 14 of 16 CR-positive asthmatics, while none of 6 CR-negative asthmatics showed bronchospasm. Late asthmatic responses also were noted in 13 of 16 CR-positive asthmatic individuals following BPT with CR antigen. The dual asthmatic reactions in CR-positive individuals were mostly inhibited by the prior administration of cromolyn sodium. Three-fold increases in peripheral eosinophil counts were noted 24 hr following BPT with CR antigen. Results indicate that CR-induced asthmatic responses are allergen-specific and CR plays a causative role in allergic asthma in the population studied.", "PMID": 965615}
{"id": "pubmed23n0032_6423", "title": "Immunologic studies in a case of baker's asthma.", "content": "A 28-year-old baker with 13 years of occupational experience has developed, over the past several years, sinusitis, rhinitis, contact dermatitis, and asthma. Clinically, all of his symptoms were felt to be related to occupational exposure. Immunologic evaluation consisted of the following tests: intradermal skin testing, leukocyte histamine release, total serum IgE, PK transfer with immunoadsorption, and specific antigen bronchial challenge. Analysis of results would tend to implicate a type I, IgE-mediated immunologic reaction.", "contents": "Immunologic studies in a case of baker's asthma. A 28-year-old baker with 13 years of occupational experience has developed, over the past several years, sinusitis, rhinitis, contact dermatitis, and asthma. Clinically, all of his symptoms were felt to be related to occupational exposure. Immunologic evaluation consisted of the following tests: intradermal skin testing, leukocyte histamine release, total serum IgE, PK transfer with immunoadsorption, and specific antigen bronchial challenge. Analysis of results would tend to implicate a type I, IgE-mediated immunologic reaction.", "PMID": 965616}
{"id": "pubmed23n0032_6424", "title": "Reagin synthesis in inbred rats. II. Genetic control of reaginic antibody synthesis.", "content": "Specific reaginic (IgE) and hemagglutinating (IgG) antibodies were quantified after immunization with ovalbumin aluminum hydroxide gel in BN and ACI inbred rats, as well as their F1, F1 X BN backcross, F2, and F3, and F3 progeny. The dissimilarity of these immune responses indicated that reaginic (IgE) antibody synthesis was influenced by polygenic factors, but not sex, and was controlled by loci different from that governing hemagglutinating (IgG) antibody synthesis. In addition, tissue typing of the BN, ACI, F1, and F3 hybrids suggested that reaginic antibody synthesis was not linked to the major rat histocompatibility locus.", "contents": "Reagin synthesis in inbred rats. II. Genetic control of reaginic antibody synthesis. Specific reaginic (IgE) and hemagglutinating (IgG) antibodies were quantified after immunization with ovalbumin aluminum hydroxide gel in BN and ACI inbred rats, as well as their F1, F1 X BN backcross, F2, and F3, and F3 progeny. The dissimilarity of these immune responses indicated that reaginic (IgE) antibody synthesis was influenced by polygenic factors, but not sex, and was controlled by loci different from that governing hemagglutinating (IgG) antibody synthesis. In addition, tissue typing of the BN, ACI, F1, and F3 hybrids suggested that reaginic antibody synthesis was not linked to the major rat histocompatibility locus.", "PMID": 965617}
{"id": "pubmed23n0032_6425", "title": "The clinical gerontologist.", "content": "A major objective now and in the future should be that of maximizing health and well-being during our essentially fixed span of life. It is proposed that this objective be delegated to a new field of medicine, perhaps to be known as clinical gerontology--a combination of preventive medicine and geriatrics.", "contents": "The clinical gerontologist. A major objective now and in the future should be that of maximizing health and well-being during our essentially fixed span of life. It is proposed that this objective be delegated to a new field of medicine, perhaps to be known as clinical gerontology--a combination of preventive medicine and geriatrics.", "PMID": 965674}
{"id": "pubmed23n0032_6426", "title": "A complex case of sarcoidosis fever.", "content": "Febrile episodes in a male patient with coronary artery disease and arteriovenous malformation of the spinal cord had been attributed to urinary-tract infections and pulmonary atelectasis. The autopsy findings, however, indicated that the fever may have been related to sarcoidosis which had not been detected pre mortem.", "contents": "A complex case of sarcoidosis fever. Febrile episodes in a male patient with coronary artery disease and arteriovenous malformation of the spinal cord had been attributed to urinary-tract infections and pulmonary atelectasis. The autopsy findings, however, indicated that the fever may have been related to sarcoidosis which had not been detected pre mortem.", "PMID": 965675}
{"id": "pubmed23n0032_6427", "title": "Sensory deprivation in geriatric patients.", "content": "Sensory deprivation speeds up the degenerative changes normally associated with aging and enhances the loss of functional cells in the central nervous system. Since it connotes reduction or absence of stimulation of the five senses, it involves the patient's physical activity, social relationships, intellectual status and overall \"value system.\" Geriatric patients with various illnesses are particularly vulnerable to sensory deprivation, which becomes increasingly pronounced as physical or mental deterioration progresses. All cells require stimulation for continued growth and activity; lack of stimulation leads to atrophy, with secondary physical or psychosocial abnormalities. Depression acts as a negative reinforcement of stimuli, and anxiety acts as a blocking mechanism. The goal of rehabilitation is to maintain and strengthen the patient's social values and to preserve mental and physical motility. The recommended procedure involves assessment of medical, psychologic and social factors, and determination of whether organic disease is present. A specific treatment plan to meet the individualized needs of each patient is then established so as to minimize sensory deprivation and maximize physical and mental functioning. Re-evaluation (physical and psychologic) at regular intervals establishes a pattern not only for individual comparison but for comparison with results in untreated control groups.", "contents": "Sensory deprivation in geriatric patients. Sensory deprivation speeds up the degenerative changes normally associated with aging and enhances the loss of functional cells in the central nervous system. Since it connotes reduction or absence of stimulation of the five senses, it involves the patient's physical activity, social relationships, intellectual status and overall \"value system.\" Geriatric patients with various illnesses are particularly vulnerable to sensory deprivation, which becomes increasingly pronounced as physical or mental deterioration progresses. All cells require stimulation for continued growth and activity; lack of stimulation leads to atrophy, with secondary physical or psychosocial abnormalities. Depression acts as a negative reinforcement of stimuli, and anxiety acts as a blocking mechanism. The goal of rehabilitation is to maintain and strengthen the patient's social values and to preserve mental and physical motility. The recommended procedure involves assessment of medical, psychologic and social factors, and determination of whether organic disease is present. A specific treatment plan to meet the individualized needs of each patient is then established so as to minimize sensory deprivation and maximize physical and mental functioning. Re-evaluation (physical and psychologic) at regular intervals establishes a pattern not only for individual comparison but for comparison with results in untreated control groups.", "PMID": 965676}
{"id": "pubmed23n0032_6428", "title": "Old-age emotions.", "content": "One hundred old people (age range 60-94 years) were studied with respect to the state of their emotions. The chief factors leading to emotional sterility were loneliness, retirement and decline in sensory and locomotory functions. Among the 100 subjects, 60 were optimists and 40 were pessimists; most of the pessimists were lonely people. Suggestions are made for combating emotional \"emptiness\" before it leads to catastrophic mental impairment.", "contents": "Old-age emotions. One hundred old people (age range 60-94 years) were studied with respect to the state of their emotions. The chief factors leading to emotional sterility were loneliness, retirement and decline in sensory and locomotory functions. Among the 100 subjects, 60 were optimists and 40 were pessimists; most of the pessimists were lonely people. Suggestions are made for combating emotional \"emptiness\" before it leads to catastrophic mental impairment.", "PMID": 965677}
{"id": "pubmed23n0032_6429", "title": "Upgrading services in the small extended care facility.", "content": "The Extended Care Facility (ECF) has become an important part of the community's provision of health care for the aged. Appropriate services must be available for long-term, intermediate, skilled, and short-term medical care. This report describes a small ECF (180 patients) rendering these needed services for the elderly. A limited operating budget, a shortage of physicians, and demand for an increase in the patient-load are some of the typical problems. In order that the important services can be rendered properly to the aged patients, participation by all the medical disciplines is essential. (The role of the Physical Therapy Department is discussed.) To upgrade services effectively, the interdisciplinary approach must involve a dual responsibility, i.e., the individual effort of the single discipline plus the group cooperative effort of all areas concerned.", "contents": "Upgrading services in the small extended care facility. The Extended Care Facility (ECF) has become an important part of the community's provision of health care for the aged. Appropriate services must be available for long-term, intermediate, skilled, and short-term medical care. This report describes a small ECF (180 patients) rendering these needed services for the elderly. A limited operating budget, a shortage of physicians, and demand for an increase in the patient-load are some of the typical problems. In order that the important services can be rendered properly to the aged patients, participation by all the medical disciplines is essential. (The role of the Physical Therapy Department is discussed.) To upgrade services effectively, the interdisciplinary approach must involve a dual responsibility, i.e., the individual effort of the single discipline plus the group cooperative effort of all areas concerned.", "PMID": 965678}
{"id": "pubmed23n0032_6430", "title": "Geriatric psycho-social history outline.", "content": "The details of a Geriatric Psycho-Social History Outline for use with the institutional aged are presented. All health care facilities require background information on the patient at the time of admission, but with long-term facilities a more comprehensive psycho-social history is needed. The Outline provides a basis for obtaining information in five categories: 1) identification (detailed, observational and attitudinal); 2) referral source; 3) background; 4) family constellation or environmental factors; and 5) finances. Its comprehensiveness requires more than one pre-admission interview. It is aimed at reducing morbidity and mortality rates following admission to long-term facilities. If the aged person becomes somewhat familiar with the institution before admission, he is less susceptible to accelerated physiologic and psycho-social deterioration and death. Because of constant growth in the field of gerontology, new and improved instruments must be devised to help carry the load. The Geriatric Psycho-Social History Outline is for use as a guide to those who require such information if they are to function at maximum efficiency.", "contents": "Geriatric psycho-social history outline. The details of a Geriatric Psycho-Social History Outline for use with the institutional aged are presented. All health care facilities require background information on the patient at the time of admission, but with long-term facilities a more comprehensive psycho-social history is needed. The Outline provides a basis for obtaining information in five categories: 1) identification (detailed, observational and attitudinal); 2) referral source; 3) background; 4) family constellation or environmental factors; and 5) finances. Its comprehensiveness requires more than one pre-admission interview. It is aimed at reducing morbidity and mortality rates following admission to long-term facilities. If the aged person becomes somewhat familiar with the institution before admission, he is less susceptible to accelerated physiologic and psycho-social deterioration and death. Because of constant growth in the field of gerontology, new and improved instruments must be devised to help carry the load. The Geriatric Psycho-Social History Outline is for use as a guide to those who require such information if they are to function at maximum efficiency.", "PMID": 965679}
{"id": "pubmed23n0032_6431", "title": "Doxorubicin (adriamycin) therapy of uterine sarcoma without surgery in an elderly patient.", "content": "A case is presented of primary endolymphatic stromal sarcoma of the uterus in a debilitated elderly patient. Surgical intervention was contraindicated. Radium therapy was unsuccessful. A 4-month course of treatment with doxorubicin (Adriamycin) resulted in disappearance of the tumor. The patient is living 19 months after the beginning of Adriamycin therapy.", "contents": "Doxorubicin (adriamycin) therapy of uterine sarcoma without surgery in an elderly patient. A case is presented of primary endolymphatic stromal sarcoma of the uterus in a debilitated elderly patient. Surgical intervention was contraindicated. Radium therapy was unsuccessful. A 4-month course of treatment with doxorubicin (Adriamycin) resulted in disappearance of the tumor. The patient is living 19 months after the beginning of Adriamycin therapy.", "PMID": 965680}
{"id": "pubmed23n0032_6432", "title": "Studies on the Malayan forest rat filaria, Breinlia booliati (Filarioidea: Onchocercidae): course of development in rat host.", "content": "The development of Breinlia booliati is described in its natural host, Rattus sabanus and in an inbred strain of laboratory albino rat. The growth of the parasite is similar in both the rat hosts. The third moult occurs between six-eight days and the final moult between 24-28 days. Larvae were recovered initially from the skin and carcass. After five weeks, developing stages were seen only in the thoracic and abdominal cavities, the site of development of the adult worms. Worms became sexually mature by 11-12 weeks and there was considerable growth in length of the female worms after this stage.", "contents": "Studies on the Malayan forest rat filaria, Breinlia booliati (Filarioidea: Onchocercidae): course of development in rat host. The development of Breinlia booliati is described in its natural host, Rattus sabanus and in an inbred strain of laboratory albino rat. The growth of the parasite is similar in both the rat hosts. The third moult occurs between six-eight days and the final moult between 24-28 days. Larvae were recovered initially from the skin and carcass. After five weeks, developing stages were seen only in the thoracic and abdominal cavities, the site of development of the adult worms. Worms became sexually mature by 11-12 weeks and there was considerable growth in length of the female worms after this stage.", "PMID": 965704}
{"id": "pubmed23n0032_6433", "title": "Preliminary observations on the distribution, definitive hosts and possible relation with other schistosomes, of Schistosoma margrebowiei, Le Roux, 1933 and Schistosoma leiperi, Le Roux, 1955.", "content": "In attempting to define the south western limits of the distribution of Schistosoma margrebowiei and S. leiperi and investigating their apparent limited distribution in relation to their snail hosts it was found that there was little overlap in the distribution of S. margrebowiei and S. leiperi and that of the human parasites S. haematobium and S. mansoni, and no overlap with S. mattheei within the area surveyed. It is suggested that the presence of either or both lechwe schistosomes prevented the development of the human parasites and in addition, prevented the development of S. mattheei in certain areas inhabited or surrounded by certain Kobus spp. The restricted distribution of S. margrebowiei and S. leiperi was thought to be due to the restricted distribution of their main definitive hosts Kobus leche and K. vardoni; the poor host susceptibility of cat and possibly Syncerus caffer; and the likelihood of most other \"common\" game being unable to maintain the parasites indefinitely outside the confines of lechwe and puku distribution in southern Africa.", "contents": "Preliminary observations on the distribution, definitive hosts and possible relation with other schistosomes, of Schistosoma margrebowiei, Le Roux, 1933 and Schistosoma leiperi, Le Roux, 1955. In attempting to define the south western limits of the distribution of Schistosoma margrebowiei and S. leiperi and investigating their apparent limited distribution in relation to their snail hosts it was found that there was little overlap in the distribution of S. margrebowiei and S. leiperi and that of the human parasites S. haematobium and S. mansoni, and no overlap with S. mattheei within the area surveyed. It is suggested that the presence of either or both lechwe schistosomes prevented the development of the human parasites and in addition, prevented the development of S. mattheei in certain areas inhabited or surrounded by certain Kobus spp. The restricted distribution of S. margrebowiei and S. leiperi was thought to be due to the restricted distribution of their main definitive hosts Kobus leche and K. vardoni; the poor host susceptibility of cat and possibly Syncerus caffer; and the likelihood of most other \"common\" game being unable to maintain the parasites indefinitely outside the confines of lechwe and puku distribution in southern Africa.", "PMID": 965705}
{"id": "pubmed23n0032_6434", "title": "Observations on the life-cycle of the strigeoid trematode, Apatemon (Apatemon) gracilis (Rudolphi, 1819) Szidat, 1928.", "content": "The life-cycle of Apatemon (A.) gracilis was completed in the laboratory. The snail host is Lymnaea peregra (Muller). The cercaria is redescribed from a wide range of material. Metacercariae were found in naturally infected rainbow trout (Salmo gairdneri Richardson), three-spined sticklebacks (Gasterosteus aculeatus L.) and stone loach (Nemacheilus barbatulus (L.)) from Scotland and in three-spined sticklebacks from Iceland. In trout, most metacercariae were found in the pericardial cavity, in sticklebacks, the eye, and in loach, the body cavity. In infection experiments, cercariae from naturally infected Scottish snails developed in three-spined sticklebacks, rainbow trout and brown trout (Salmo trutta L.). Under experimental conditions cercariae did not penetrate stone loach, although this species is naturally infected with A. gracilis. The phenomenon of fish host specificity is briefly discussed. Development of the metacercaria is described. Excystation of metacercarial cysts with pepsin and trypsin solutions is unlike that reported for any other digenean; the contents of the cyst appear to be under pressure. In pepsin, layers of the cyst wall peel back from one end. When transferred to trypsin, one pole of the cyst ruptures and the worm is forcibly expelled.", "contents": "Observations on the life-cycle of the strigeoid trematode, Apatemon (Apatemon) gracilis (Rudolphi, 1819) Szidat, 1928. The life-cycle of Apatemon (A.) gracilis was completed in the laboratory. The snail host is Lymnaea peregra (Muller). The cercaria is redescribed from a wide range of material. Metacercariae were found in naturally infected rainbow trout (Salmo gairdneri Richardson), three-spined sticklebacks (Gasterosteus aculeatus L.) and stone loach (Nemacheilus barbatulus (L.)) from Scotland and in three-spined sticklebacks from Iceland. In trout, most metacercariae were found in the pericardial cavity, in sticklebacks, the eye, and in loach, the body cavity. In infection experiments, cercariae from naturally infected Scottish snails developed in three-spined sticklebacks, rainbow trout and brown trout (Salmo trutta L.). Under experimental conditions cercariae did not penetrate stone loach, although this species is naturally infected with A. gracilis. The phenomenon of fish host specificity is briefly discussed. Development of the metacercaria is described. Excystation of metacercarial cysts with pepsin and trypsin solutions is unlike that reported for any other digenean; the contents of the cyst appear to be under pressure. In pepsin, layers of the cyst wall peel back from one end. When transferred to trypsin, one pole of the cyst ruptures and the worm is forcibly expelled.", "PMID": 965706}
{"id": "pubmed23n0032_6435", "title": "A demonstration of the presence of anti-snail antibodies in individuals infected with Schistosoma haematobium.", "content": "An extract of the snail Bulinus (Physopsis) africanus, the intermediate host of Schistosoma haematobium, was used as the antigen in haemagglutination tests in a survey of the prevalence of anti-snail antibodies in various population groups. It was found that sera from known bilharzia-infected individuals and randomly selected individuals from bilharzia endemic areas had significantly higher incidences as well as higher titres of antibodies to this snail antigen than non-infected individuals and individuals from non-endemic areas.", "contents": "A demonstration of the presence of anti-snail antibodies in individuals infected with Schistosoma haematobium. An extract of the snail Bulinus (Physopsis) africanus, the intermediate host of Schistosoma haematobium, was used as the antigen in haemagglutination tests in a survey of the prevalence of anti-snail antibodies in various population groups. It was found that sera from known bilharzia-infected individuals and randomly selected individuals from bilharzia endemic areas had significantly higher incidences as well as higher titres of antibodies to this snail antigen than non-infected individuals and individuals from non-endemic areas.", "PMID": 965707}
{"id": "pubmed23n0032_6436", "title": "The epidemiology of Echinococcus infection in Kuwait.", "content": "Infection of dogs with E. granulosus is recorded for the first time in the State of Kuwait and observations on 204 dogs from different suburbs showed an infection rate of 23.039%. Cattle, sheep, goats and camels killed for public consumption in the period 6th April to 6th May, 1975, in a slaughter house were inspected and the rate of infection with E. granulosus cysts was recorded. The possible methods of transmission from animals and dogs to man is discussed.", "contents": "The epidemiology of Echinococcus infection in Kuwait. Infection of dogs with E. granulosus is recorded for the first time in the State of Kuwait and observations on 204 dogs from different suburbs showed an infection rate of 23.039%. Cattle, sheep, goats and camels killed for public consumption in the period 6th April to 6th May, 1975, in a slaughter house were inspected and the rate of infection with E. granulosus cysts was recorded. The possible methods of transmission from animals and dogs to man is discussed.", "PMID": 965708}
{"id": "pubmed23n0032_6437", "title": "Developing protoscoleces of Echinococcus granulosus on the outer surface of the brood capsule, detected by scanning electron microscopy.", "content": "By means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules of Echinococcus granulosus. Histological studies showed these protrusions to be developing protoscoleces. However, complete development is not attained and the protoscoleces eventually die. It is suggested that external development is a result of overcrowding within the brood capsule.", "contents": "Developing protoscoleces of Echinococcus granulosus on the outer surface of the brood capsule, detected by scanning electron microscopy. By means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules of Echinococcus granulosus. Histological studies showed these protrusions to be developing protoscoleces. However, complete development is not attained and the protoscoleces eventually die. It is suggested that external development is a result of overcrowding within the brood capsule.", "PMID": 965709}
{"id": "pubmed23n0032_6438", "title": "The occurrence of Mesocestoides sp. in British wild red foxes (Vulpes vulpes crucigera).", "content": "Infection with the cyclophyllidean tapeworm Mesocestoides sp. is recorded in British wild red foxes (Vulpes vulpes cr-cigera) from Scotland and South East England. Previously, the occurence of this parasite in Great Britain has been rarely reported, but the results of the present study indicate that vulpine infection may be common.", "contents": "The occurrence of Mesocestoides sp. in British wild red foxes (Vulpes vulpes crucigera). Infection with the cyclophyllidean tapeworm Mesocestoides sp. is recorded in British wild red foxes (Vulpes vulpes cr-cigera) from Scotland and South East England. Previously, the occurence of this parasite in Great Britain has been rarely reported, but the results of the present study indicate that vulpine infection may be common.", "PMID": 965710}
{"id": "pubmed23n0032_6439", "title": "Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.", "content": "Oxidation of tissue sections by 25-30% (v/v) acetic anhydride (AA) in dimethyl sulfoxide (DMSO) resulted in facile induction of tissue carbonyls readily localized with Schiff's reagent and o-dianisidine but not with the 3-hydroxy-2-naphthoic acid hydrazide-tetraazotized diorthoanisidine method. Carbonyls generated by AA-DMSO oxidation were confined predomintly to substrates containing pyranosides. Oxidized furanosides, as represented by deoxyribonucleic acid and ribonucleic acid, gave only a residual color reaction. The AA-DMSO method possesses an advantage in that the oxidation of tissue polysaccharides does not proceed beyond the formation of carbonyly and is particularly suited for use after formol fixation.", "contents": "Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide. Oxidation of tissue sections by 25-30% (v/v) acetic anhydride (AA) in dimethyl sulfoxide (DMSO) resulted in facile induction of tissue carbonyls readily localized with Schiff's reagent and o-dianisidine but not with the 3-hydroxy-2-naphthoic acid hydrazide-tetraazotized diorthoanisidine method. Carbonyls generated by AA-DMSO oxidation were confined predomintly to substrates containing pyranosides. Oxidized furanosides, as represented by deoxyribonucleic acid and ribonucleic acid, gave only a residual color reaction. The AA-DMSO method possesses an advantage in that the oxidation of tissue polysaccharides does not proceed beyond the formation of carbonyly and is particularly suited for use after formol fixation.", "PMID": 965716}
{"id": "pubmed23n0032_6440", "title": "[Quantitative growth analysis of limbic nuclei areas fresh volume in diencephalon and mesencephalon of an albino mouse ontogenic series. I. Nucleus habenulare].", "content": "The fresh volumes of the Nucl. mediales et laterales habenulae of 52 male white mice aged between 17 and 60 days of ontogenesis have been determined. The data of both nuclei have been fitted by a sum of logistic functions. There is an increasing component and a decreasing component. The degree of maturity and its differential quotient have been discussed. The Nucl. medialis habenulae belongs to those structures of the central nervous system developing extremely early, the Nucl. lateralis habenulae belongs to those structures of the central nervous system developing extremely early, the Nucl. lateralis habenulae belongs to those structures developing early. It has been pointed out, that there is a complex heterochrony of regional development in the brain. There have to be more quantitative analyses.", "contents": "[Quantitative growth analysis of limbic nuclei areas fresh volume in diencephalon and mesencephalon of an albino mouse ontogenic series. I. Nucleus habenulare]. The fresh volumes of the Nucl. mediales et laterales habenulae of 52 male white mice aged between 17 and 60 days of ontogenesis have been determined. The data of both nuclei have been fitted by a sum of logistic functions. There is an increasing component and a decreasing component. The degree of maturity and its differential quotient have been discussed. The Nucl. medialis habenulae belongs to those structures of the central nervous system developing extremely early, the Nucl. lateralis habenulae belongs to those structures of the central nervous system developing extremely early, the Nucl. lateralis habenulae belongs to those structures developing early. It has been pointed out, that there is a complex heterochrony of regional development in the brain. There have to be more quantitative analyses.", "PMID": 965717}
{"id": "pubmed23n0032_6441", "title": "[Quantitative growth analysis of limbic nuclei areas fresh volume in diencephalon and mesencephalon of an albino mouse ontogenic series. III. Nucleus interpe-uncularis].", "content": "The fresh volume of the Nucl. interpeduncularis of 52 white mice aged between 17 and 60 days of ontogenesis has been determined. The data have been fitted by the logistic function and the ideal volume (0.32 mm3) the half value time (21.6 days of ontogenesis) and the factor of enlargement (2.65) have been derived. The Nucl. interpeduncularis is one of the early and fast growing brain regions. The growth parameters of this nucleus have been discussed and have been compared with the parameters of other cortical and subcortical limbic brain regions.", "contents": "[Quantitative growth analysis of limbic nuclei areas fresh volume in diencephalon and mesencephalon of an albino mouse ontogenic series. III. Nucleus interpe-uncularis]. The fresh volume of the Nucl. interpeduncularis of 52 white mice aged between 17 and 60 days of ontogenesis has been determined. The data have been fitted by the logistic function and the ideal volume (0.32 mm3) the half value time (21.6 days of ontogenesis) and the factor of enlargement (2.65) have been derived. The Nucl. interpeduncularis is one of the early and fast growing brain regions. The growth parameters of this nucleus have been discussed and have been compared with the parameters of other cortical and subcortical limbic brain regions.", "PMID": 965718}
{"id": "pubmed23n0032_6442", "title": "[Morphometric-statistical structure analysis of human striatum, pallidum and nucleus su-thalamicus. III. Nucleus subthalamicus].", "content": "1. A morphometric-statistical analysis of the subthalamic nucleus was carried out on 14 hemispheres of 12 normal human brains. 2. The values determined in paraffin embedded sections were corrected for shrinkage which showed considerable interindividual variation. 3. The mean fresh volume of the subthalamic nucleus amounted to 144 mm3 for males and 134 mm3 for females, the difference of 7% not being statistically significant. 4. In the medial part of the subthalamic nucleus the nerve cells were smaller and more closely grouped than in the lateral part which had larger nerve cells. The ratio of the volumes of the two parts was about 1:4 (medial: lateral). 5. The nucleus subthalamicus occupied 0,027% of the volume of the hemisphere. 6. The numerical nerve cell densities -- corrected for shrinkage -- were 1970 +/- 145 nerve cells/mm3 for the lateral part and 2910 +/- 310 nerve cells/mm3 for the medial part, this difference of 48 +/- 4% being highly significant. On the other hand, no difference between sexes could be shown. 7. The mean of the absolute number of nerve cells was 306000 for male and 286000 for females, the difference of 7% not being significant. 8. The volumetric nerve cell densities were found to be 1.58 +/- 0.15 vol% lateral and 1.99 +/- 0.16 vol% medial, the difference of 27% being significant. No differences between sexes were found. 9. The linear dimensions of the nerve cells of the medial part were about 10% smaller than those of the nerve cells of the lateral part. The mean fresh volume of a nerve cell was 8070 mum3 in the lateral portion and 6960 mum3 in the medial portion, the nerve cells of pars lateralis being just as large as the nerve cells of the lateral segment of the pallidum (8100 mum3). 10. The parameters determined showed good correlations with corresponding data of the pallidum. The fresh volume and the absolute numbers of nerve cells of the nucleus subthalamicus were better correlated with the pallidum laterale than with the pallidum mediale.", "contents": "[Morphometric-statistical structure analysis of human striatum, pallidum and nucleus su-thalamicus. III. Nucleus subthalamicus]. 1. A morphometric-statistical analysis of the subthalamic nucleus was carried out on 14 hemispheres of 12 normal human brains. 2. The values determined in paraffin embedded sections were corrected for shrinkage which showed considerable interindividual variation. 3. The mean fresh volume of the subthalamic nucleus amounted to 144 mm3 for males and 134 mm3 for females, the difference of 7% not being statistically significant. 4. In the medial part of the subthalamic nucleus the nerve cells were smaller and more closely grouped than in the lateral part which had larger nerve cells. The ratio of the volumes of the two parts was about 1:4 (medial: lateral). 5. The nucleus subthalamicus occupied 0,027% of the volume of the hemisphere. 6. The numerical nerve cell densities -- corrected for shrinkage -- were 1970 +/- 145 nerve cells/mm3 for the lateral part and 2910 +/- 310 nerve cells/mm3 for the medial part, this difference of 48 +/- 4% being highly significant. On the other hand, no difference between sexes could be shown. 7. The mean of the absolute number of nerve cells was 306000 for male and 286000 for females, the difference of 7% not being significant. 8. The volumetric nerve cell densities were found to be 1.58 +/- 0.15 vol% lateral and 1.99 +/- 0.16 vol% medial, the difference of 27% being significant. No differences between sexes were found. 9. The linear dimensions of the nerve cells of the medial part were about 10% smaller than those of the nerve cells of the lateral part. The mean fresh volume of a nerve cell was 8070 mum3 in the lateral portion and 6960 mum3 in the medial portion, the nerve cells of pars lateralis being just as large as the nerve cells of the lateral segment of the pallidum (8100 mum3). 10. The parameters determined showed good correlations with corresponding data of the pallidum. The fresh volume and the absolute numbers of nerve cells of the nucleus subthalamicus were better correlated with the pallidum laterale than with the pallidum mediale.", "PMID": 965719}
{"id": "pubmed23n0032_6443", "title": "The nuclei of the vestibular nerve of the red kangaroo, Megaleia rufus.", "content": "The preceeding account describes vestibular nuclei of the kangaroo which conform to the general mammalian pattern. Certain pecularities such as subdivisions of the medial vestibular nuclei are reported but cannot be assigned functional importance. The four major vestibular nuclei and the cerebellum receive vestibular root fibers in like manner as in other mammalian brains. The nuclear patterns and the secondary connections of the vestibular complex in the kangaroo are likewise consistent with those described in other mammals. This is so evident from the description of these regions in the kangaroo and the reports in the literature that the statement needs no further documentation. However, there are certain differences which are significant. Unusual size and/or differentiation of a center, together with an increase in the projection path arising from it, is often reflected in the behavioral patterns of the animal. Thus the lateral vestibular nucleus and the ventrolateral vestibulospinal tract are conspicuously large in the kangaroo in which the methods of locomotion and the manner in which he fights must require frequent orientation in space. It is obvious that the locomotion capabilities of the kangaroo are in large measure due to his well developed vestibular complex and its descending connection. Studies of the sort represented here, then, emphasize the specializations in certain systems and provide information with regard to the interrelations between the anatomic patterns and functional specilization in these systems. They also supplement our knowledge of the course of differentiation within the mammalian phylum. It is to be noticed that specialization at a given level may occur at any place in the evolutionary scale but cephalization is an indication of a relatively progressive development from lower to higher forms.", "contents": "The nuclei of the vestibular nerve of the red kangaroo, Megaleia rufus. The preceeding account describes vestibular nuclei of the kangaroo which conform to the general mammalian pattern. Certain pecularities such as subdivisions of the medial vestibular nuclei are reported but cannot be assigned functional importance. The four major vestibular nuclei and the cerebellum receive vestibular root fibers in like manner as in other mammalian brains. The nuclear patterns and the secondary connections of the vestibular complex in the kangaroo are likewise consistent with those described in other mammals. This is so evident from the description of these regions in the kangaroo and the reports in the literature that the statement needs no further documentation. However, there are certain differences which are significant. Unusual size and/or differentiation of a center, together with an increase in the projection path arising from it, is often reflected in the behavioral patterns of the animal. Thus the lateral vestibular nucleus and the ventrolateral vestibulospinal tract are conspicuously large in the kangaroo in which the methods of locomotion and the manner in which he fights must require frequent orientation in space. It is obvious that the locomotion capabilities of the kangaroo are in large measure due to his well developed vestibular complex and its descending connection. Studies of the sort represented here, then, emphasize the specializations in certain systems and provide information with regard to the interrelations between the anatomic patterns and functional specilization in these systems. They also supplement our knowledge of the course of differentiation within the mammalian phylum. It is to be noticed that specialization at a given level may occur at any place in the evolutionary scale but cephalization is an indication of a relatively progressive development from lower to higher forms.", "PMID": 965720}
{"id": "pubmed23n0032_6444", "title": "[Ventromedial field of human tuber cinereum. Mechanical morphometry].", "content": "A quantitative structural analysis of the ventromedial area of the human hypothalamus was performed using an automatic image analysing computer. We divided the ventromedial area into two nuclei, nucleus ventromedialis (N. vm.) and nucleus infundibularis (N. inf.). N. vm. was studied in 6 cases, N. inf. in 7 cases. The following findings were obtained: 1. No significant differences of cell population became apparent between the left and right sides of both nuclei. Aside from a female case above 80 years of age, so far, no correlation with cellular distribution of age and sex, respectively, could be established. 2. In N. vm. the distribution of large and small nerve cells depends on the region within this nucleus. Small nerve cells appear more frequent in the medial region than in the central and in the lateral regions. On the other hand, significant distributional differences of nerve cells in the dorsal, central, and basal regions as well as in oro-caudal direction are absent. Data of individual cases and their mean value are conforming. Cell density (cell number/volume) does not show any regional dependence both in the frontal plane and in the oro-caudal direction; it amounts to approximately 170/mum3 in the entire nucleus. 3. In the frontal plane plane N. inf. does not disclose marked regional differences of cell population. Large neurons are slightly more frequent in the basal-medial region as compared to the dorsal region. An 81-year-old woman displayed a conspicuous increase of large nerve cells in the basal and centrolateral regions, obviously hypertrophy of the potential \"subventricular nucleus\" as described by SHEEHAN and KOVACS, which is, in our opinion, a part of the N. inf. In young subjects (below the age of 40 years) the frequency of large nerve cells decreases in oro-caudal direction. Identical cell populations suggest that the cuadal end of N. inf. extends to the floor of the mammillary recessus. Cell density in the frontal plane appears higher in the centrolateral region (230/mum3) compared to the dorsal and basal regions (190-200/mum3). Cell density increases in oro-caudal extent; it decreases, however, in the most caudal part.", "contents": "[Ventromedial field of human tuber cinereum. Mechanical morphometry]. A quantitative structural analysis of the ventromedial area of the human hypothalamus was performed using an automatic image analysing computer. We divided the ventromedial area into two nuclei, nucleus ventromedialis (N. vm.) and nucleus infundibularis (N. inf.). N. vm. was studied in 6 cases, N. inf. in 7 cases. The following findings were obtained: 1. No significant differences of cell population became apparent between the left and right sides of both nuclei. Aside from a female case above 80 years of age, so far, no correlation with cellular distribution of age and sex, respectively, could be established. 2. In N. vm. the distribution of large and small nerve cells depends on the region within this nucleus. Small nerve cells appear more frequent in the medial region than in the central and in the lateral regions. On the other hand, significant distributional differences of nerve cells in the dorsal, central, and basal regions as well as in oro-caudal direction are absent. Data of individual cases and their mean value are conforming. Cell density (cell number/volume) does not show any regional dependence both in the frontal plane and in the oro-caudal direction; it amounts to approximately 170/mum3 in the entire nucleus. 3. In the frontal plane plane N. inf. does not disclose marked regional differences of cell population. Large neurons are slightly more frequent in the basal-medial region as compared to the dorsal region. An 81-year-old woman displayed a conspicuous increase of large nerve cells in the basal and centrolateral regions, obviously hypertrophy of the potential \"subventricular nucleus\" as described by SHEEHAN and KOVACS, which is, in our opinion, a part of the N. inf. In young subjects (below the age of 40 years) the frequency of large nerve cells decreases in oro-caudal direction. Identical cell populations suggest that the cuadal end of N. inf. extends to the floor of the mammillary recessus. Cell density in the frontal plane appears higher in the centrolateral region (230/mum3) compared to the dorsal and basal regions (190-200/mum3). Cell density increases in oro-caudal extent; it decreases, however, in the most caudal part.", "PMID": 965721}
{"id": "pubmed23n0032_6445", "title": "[Conditioning and synapse density. Morphometry of stratum lacunosum-moleculare of rat hippocampal CA1-region].", "content": "Male albino rats aged 13 weeks experienced a single training for bright -- dark discrimination in a Y-chamber. The numerical density of synapses determined in the stratum lacunosum-moleculare of the hippocampal area CA1 24 hours later turned out to be lower by 3.4 per cent with trained animals compared to passive controls. This difference failed, however, to prove significant.", "contents": "[Conditioning and synapse density. Morphometry of stratum lacunosum-moleculare of rat hippocampal CA1-region]. Male albino rats aged 13 weeks experienced a single training for bright -- dark discrimination in a Y-chamber. The numerical density of synapses determined in the stratum lacunosum-moleculare of the hippocampal area CA1 24 hours later turned out to be lower by 3.4 per cent with trained animals compared to passive controls. This difference failed, however, to prove significant.", "PMID": 965722}
{"id": "pubmed23n0032_6446", "title": "Distribution and organization of spino-reticular afferents in the brain stem of rat.", "content": "The spino-reticular projection was studied, using the silver impregnation method according to NAUTA in 15 rats with various lesions in the spinal cord. The brains were cut in frontal, sagittal or horizontal sections. Two bundles were found projecting from the spinal cord into the reticular formation: The dorso-medial bundle contains independent spino-reticular fibres which terminate in the nucleus reticularis lateralis and in the medial nuclei of the reticular formation of the rhombencephalon (this bundle is topographically organized). The spino-reticular projection to the mesencephalon runs separately in the ventro-lateral bundle, which is a complex of spino-cerebellar, spino-tectal and spino-thalamic pathways. The spino-reticular projection to the mesencephalon is is diffuse and has no sign of organization as opposed to the dorso-medial spino-reticular tract.", "contents": "Distribution and organization of spino-reticular afferents in the brain stem of rat. The spino-reticular projection was studied, using the silver impregnation method according to NAUTA in 15 rats with various lesions in the spinal cord. The brains were cut in frontal, sagittal or horizontal sections. Two bundles were found projecting from the spinal cord into the reticular formation: The dorso-medial bundle contains independent spino-reticular fibres which terminate in the nucleus reticularis lateralis and in the medial nuclei of the reticular formation of the rhombencephalon (this bundle is topographically organized). The spino-reticular projection to the mesencephalon runs separately in the ventro-lateral bundle, which is a complex of spino-cerebellar, spino-tectal and spino-thalamic pathways. The spino-reticular projection to the mesencephalon is is diffuse and has no sign of organization as opposed to the dorso-medial spino-reticular tract.", "PMID": 965723}
{"id": "pubmed23n0032_6447", "title": "An attempt at determining the origin of the reticulo-spinal tract in the rat.", "content": "A search was made for retrograde changes in the cells of the reticular formation of the rhombencephalon and mesencephalon in 26 rats aged 7 to 32 days after lesions in different spinal cord segments with survival periods of 3 to 14 days. No retrograde changes were found in any of the animals. Negative results yieled by the method of retrograde degeneration appeared to show that the main mass of the axoplasm of the reticular cells in the rat aimed to other brain stem structures or in the ascending direction.", "contents": "An attempt at determining the origin of the reticulo-spinal tract in the rat. A search was made for retrograde changes in the cells of the reticular formation of the rhombencephalon and mesencephalon in 26 rats aged 7 to 32 days after lesions in different spinal cord segments with survival periods of 3 to 14 days. No retrograde changes were found in any of the animals. Negative results yieled by the method of retrograde degeneration appeared to show that the main mass of the axoplasm of the reticular cells in the rat aimed to other brain stem structures or in the ascending direction.", "PMID": 965724}
{"id": "pubmed23n0032_6448", "title": "[Quantitative study of primitive pyramidal cells in rat anterior cingular cortex].", "content": "In the present paper the primitive pyramidal cells of the Vthlayer of the anterior cingulate cortex in adult male white rats were analyzed quantitatively and compared statisticaly with large pyramidal cells of the same region. The number of dendrites, the total lengths of dendrites, the number of spines and the density of spines -- according to the order of dendrites -- show similarity between the primitive pyramidal cells and the large pyramidal cells the latter one exhibit the higher values. The curves of distribution of the various density of spines along the apical main dendrite of both cell types are similar in shape, too. The lengths of the dendritic fields and their basal spines-values are without significant distinction for both cell types, however there are more dendritic fields in large pyramidal cells. Refered to a complete pyramidal neuron they can say: there are significantly higher values in large pyramidal cells for the number of dendrites and their total lengths, the total number of spines, the number of branching sites sites and free endings. However the density of spines of the complete neuron has no significant differences between primitive and large pyramidal cells.", "contents": "[Quantitative study of primitive pyramidal cells in rat anterior cingular cortex]. In the present paper the primitive pyramidal cells of the Vthlayer of the anterior cingulate cortex in adult male white rats were analyzed quantitatively and compared statisticaly with large pyramidal cells of the same region. The number of dendrites, the total lengths of dendrites, the number of spines and the density of spines -- according to the order of dendrites -- show similarity between the primitive pyramidal cells and the large pyramidal cells the latter one exhibit the higher values. The curves of distribution of the various density of spines along the apical main dendrite of both cell types are similar in shape, too. The lengths of the dendritic fields and their basal spines-values are without significant distinction for both cell types, however there are more dendritic fields in large pyramidal cells. Refered to a complete pyramidal neuron they can say: there are significantly higher values in large pyramidal cells for the number of dendrites and their total lengths, the total number of spines, the number of branching sites sites and free endings. However the density of spines of the complete neuron has no significant differences between primitive and large pyramidal cells.", "PMID": 965725}
{"id": "pubmed23n0032_6449", "title": "[Quantitative study of dendritic tree of large (regular) pyramidal cells of lamina V in rat anterior cingular cortex].", "content": "At three month old male rats the dendritic trees of 36 large pyramidal cells in the Vth layer of the anterior cingulate cortex were analyzed quantitatively by means of the Golgi-Kopsch method. 12 pyramidal cells were localized at the medial border of the regio praecentralis (neocortex), 12 cells were localized in the adjacent mesoarchicortex, 12 cells were localized in the mesoarchicortex, which are three subregions of the anterior cingulate cortex. By means of a varianz-analysis the values of the three subregions were compared, in order to verify a supposed gradient of differentiation. 1. In the three subregions basal the number and the branching behaviour of the dendrites are greater than apical. The pyramidal cells of the neocortex and of the mesoneocortex have significantly more basal dendrites of the 1st, 2nd, 3rd, and 4th order than those of the mesoarchocortex. There are significantly more apical dendrites of the 1st and 2nd order at pyramidal cells of the neocortex compared with the two other subregions. 2. The total lengths of the dendritic branches are in proportion to the dendritic numbers of the corresponding orders. There are following tendencies for the three subregions: the number and length of apical dendrites decrease with the increasing number of order, basally, however, the number and length first increase and than decrease. 3. The total length of all basal dendrites of a pyramidal cell is largest in the mesoneocortex, than follows the neocortex, least values they find in the mesoarchicortex. There are significant differences between every one of the three subregions. The values of the total dendritic tree of a pyramidal cell (total dendritic length, total number of dendrites, volume of the dendritic tree, number of branching sites and free endings, total apical dendritic length) show significant differences between neocortex and mesoarchicortex as well as between mesoneocortex and mesoarchicortex. There are no significant differences between noecortex and mesoneocortex. The values of the total dendritic tree demonstrate that neocortex and mesoneocortex have a similar organization, while the lamina V-pyramidal cells of the mesoarchicortex indicate a significantly smaller and less branched dendritic tree.", "contents": "[Quantitative study of dendritic tree of large (regular) pyramidal cells of lamina V in rat anterior cingular cortex]. At three month old male rats the dendritic trees of 36 large pyramidal cells in the Vth layer of the anterior cingulate cortex were analyzed quantitatively by means of the Golgi-Kopsch method. 12 pyramidal cells were localized at the medial border of the regio praecentralis (neocortex), 12 cells were localized in the adjacent mesoarchicortex, 12 cells were localized in the mesoarchicortex, which are three subregions of the anterior cingulate cortex. By means of a varianz-analysis the values of the three subregions were compared, in order to verify a supposed gradient of differentiation. 1. In the three subregions basal the number and the branching behaviour of the dendrites are greater than apical. The pyramidal cells of the neocortex and of the mesoneocortex have significantly more basal dendrites of the 1st, 2nd, 3rd, and 4th order than those of the mesoarchocortex. There are significantly more apical dendrites of the 1st and 2nd order at pyramidal cells of the neocortex compared with the two other subregions. 2. The total lengths of the dendritic branches are in proportion to the dendritic numbers of the corresponding orders. There are following tendencies for the three subregions: the number and length of apical dendrites decrease with the increasing number of order, basally, however, the number and length first increase and than decrease. 3. The total length of all basal dendrites of a pyramidal cell is largest in the mesoneocortex, than follows the neocortex, least values they find in the mesoarchicortex. There are significant differences between every one of the three subregions. The values of the total dendritic tree of a pyramidal cell (total dendritic length, total number of dendrites, volume of the dendritic tree, number of branching sites and free endings, total apical dendritic length) show significant differences between neocortex and mesoarchicortex as well as between mesoneocortex and mesoarchicortex. There are no significant differences between noecortex and mesoneocortex. The values of the total dendritic tree demonstrate that neocortex and mesoneocortex have a similar organization, while the lamina V-pyramidal cells of the mesoarchicortex indicate a significantly smaller and less branched dendritic tree.", "PMID": 965726}
{"id": "pubmed23n0032_6450", "title": "Enhanced E- and EAC-rosette formation by neuraminidase.", "content": "Pre-treatment by neuraminidase of lymphocytes obtained from peripheral blood of normal donors significantly enhanced E- and EAC-rosette formation. Of other lymphoid cells only spleen cells showed significant enhancement of E-rosettes. The EAC-rosettes slightly increased when the peripheral blood lymphocytes from patients with acute lymphoblastic leukemia or chronic lymphocytic leukemia and MOLT-4 lymphoid cells were pre-treated with this enzyme. The EAC-rosettes were not increased by neuraminidase treatment of phytohemagglutinin-induced blasts, thymus cells or spleen cells. Pre-treatment of peripheral blood lymphocytes with neuraminidase also increased the proportion of stable E-rosettes resistant to incubation at 37 degrees C and to vigorous shaking. Various concentrations of neuraminidase (1-100 U/ml) produced enhancement of E- and EAC-rosettes with the highest activity at 25 and 50 U/ml. Neuraminidase treatment of sheep red blood cells failed to increase the proportion of E-rosettes of peripheral blood lymphocytes. The increased rosette forming capacity induced by neuraminidase is probably related to changes in lymphocyte surface properties.", "contents": "Enhanced E- and EAC-rosette formation by neuraminidase. Pre-treatment by neuraminidase of lymphocytes obtained from peripheral blood of normal donors significantly enhanced E- and EAC-rosette formation. Of other lymphoid cells only spleen cells showed significant enhancement of E-rosettes. The EAC-rosettes slightly increased when the peripheral blood lymphocytes from patients with acute lymphoblastic leukemia or chronic lymphocytic leukemia and MOLT-4 lymphoid cells were pre-treated with this enzyme. The EAC-rosettes were not increased by neuraminidase treatment of phytohemagglutinin-induced blasts, thymus cells or spleen cells. Pre-treatment of peripheral blood lymphocytes with neuraminidase also increased the proportion of stable E-rosettes resistant to incubation at 37 degrees C and to vigorous shaking. Various concentrations of neuraminidase (1-100 U/ml) produced enhancement of E- and EAC-rosettes with the highest activity at 25 and 50 U/ml. Neuraminidase treatment of sheep red blood cells failed to increase the proportion of E-rosettes of peripheral blood lymphocytes. The increased rosette forming capacity induced by neuraminidase is probably related to changes in lymphocyte surface properties.", "PMID": 965728}
{"id": "pubmed23n0032_6451", "title": "A method for tissue extraction and determination of prostate concentrations of endogenous androgens by radioimmunoassay.", "content": "A method for simultaneously determining concentrations of major androgens in prostate has been developed. Extraction techniques used to isolate the androgens from minced tissue include homogenization with high-speed blades in Delsal's solvent mixture, adsorption to silica gel, followed by column and one thin-layer chromatography (TLC). Radioimmunoassays (RIA) of small aliquots of TLC eluates are used to quantitate picogram amounts of 5alpha-dihydrotestosterone (DHT) and 5alpha-androstanediols (Diol) and to estimate testosterone (T) and androstenedione (Ad). Contamination of blanks was reduced to RIA sensitivity limits primarily by treatment of glassware in a self-cleaning oven. The specificity of the method for each androgen was established by TLC separations of known prostate metabolites, antisera specificities, and parallelism of sample aliquots to androgen RIA standards. The overall precision, in terms of coefficients of variation, was 21% for DHT and 24% for Diol. T and Ad could not be measured with acceptable precision because their very low concentrations in prostate (less than or equal 0.5 ng/g tissue) were less than RIA sensitivity limits. Accuracy studies indicated recoveries ranging from 96% for Diol to 121% for DHT. In human benign hypertrophic prostate tissue, DHT averaged 153 ng/g soluble protein (5.8 ng/g tissue) which was 17 times higher than values obtained in human spleen and kidney; Diol in prostate showed no consistent differences from values noted in kidney or spleen.", "contents": "A method for tissue extraction and determination of prostate concentrations of endogenous androgens by radioimmunoassay. A method for simultaneously determining concentrations of major androgens in prostate has been developed. Extraction techniques used to isolate the androgens from minced tissue include homogenization with high-speed blades in Delsal's solvent mixture, adsorption to silica gel, followed by column and one thin-layer chromatography (TLC). Radioimmunoassays (RIA) of small aliquots of TLC eluates are used to quantitate picogram amounts of 5alpha-dihydrotestosterone (DHT) and 5alpha-androstanediols (Diol) and to estimate testosterone (T) and androstenedione (Ad). Contamination of blanks was reduced to RIA sensitivity limits primarily by treatment of glassware in a self-cleaning oven. The specificity of the method for each androgen was established by TLC separations of known prostate metabolites, antisera specificities, and parallelism of sample aliquots to androgen RIA standards. The overall precision, in terms of coefficients of variation, was 21% for DHT and 24% for Diol. T and Ad could not be measured with acceptable precision because their very low concentrations in prostate (less than or equal 0.5 ng/g tissue) were less than RIA sensitivity limits. Accuracy studies indicated recoveries ranging from 96% for Diol to 121% for DHT. In human benign hypertrophic prostate tissue, DHT averaged 153 ng/g soluble protein (5.8 ng/g tissue) which was 17 times higher than values obtained in human spleen and kidney; Diol in prostate showed no consistent differences from values noted in kidney or spleen.", "PMID": 965729}
{"id": "pubmed23n0032_6452", "title": "Use and abuse of sepharose-conjugated antibodies for the isolation of lymphocyte-surface immunoglobulins.", "content": "Immunoadsorbents of Sepharose-4B-conjugated antibodies were shown to be suitable for the characterization, by subsequent SDS-polyacrylamide gel electrophoresis, of splenocyte-membrane Immunoglobulin (Ig) solubilized by detergent lysis of surface 125I-labelled cells. However, high non-specific binding of 125I-labelled lymphocyte-membrane components to Sepharose-4B prevented accurate quantition of Ig in such lysates. 125I-labelled lymphocyte-membrane components solubilized by metabolic release also showed high non-specific binding to Sepharose-4B, and this interfered with both quantitative and qualitative analysis of Ig solubilized in this manner. Initial attempts to overcome the nonspecific binding of 125I-labelled lymphocyte-membrane components to Sepharose-4B were unsuccessful, and attention is drawn to the technical problems of using Sepharose-4B as a matrix for solid-phase immunoadsorbent studies of lymphocyte-membrane Ig.", "contents": "Use and abuse of sepharose-conjugated antibodies for the isolation of lymphocyte-surface immunoglobulins. Immunoadsorbents of Sepharose-4B-conjugated antibodies were shown to be suitable for the characterization, by subsequent SDS-polyacrylamide gel electrophoresis, of splenocyte-membrane Immunoglobulin (Ig) solubilized by detergent lysis of surface 125I-labelled cells. However, high non-specific binding of 125I-labelled lymphocyte-membrane components to Sepharose-4B prevented accurate quantition of Ig in such lysates. 125I-labelled lymphocyte-membrane components solubilized by metabolic release also showed high non-specific binding to Sepharose-4B, and this interfered with both quantitative and qualitative analysis of Ig solubilized in this manner. Initial attempts to overcome the nonspecific binding of 125I-labelled lymphocyte-membrane components to Sepharose-4B were unsuccessful, and attention is drawn to the technical problems of using Sepharose-4B as a matrix for solid-phase immunoadsorbent studies of lymphocyte-membrane Ig.", "PMID": 965730}
{"id": "pubmed23n0032_6453", "title": "Acridine orange fluorescence cytochemistry for detecting lymphocyte immunoreactivity.", "content": "Acridine orange staining reveals changes within 3 hours of in vitro stimulation of normal rat lymphocytes with mitogens, and of immune rat lymphocytes with the sensitizing antigen. An increased number of red fluorescent cytoplasmic organelles, presumably lysosomes are seen by fluorescence microscopy. Fluorimetry of the supernatants from stained cell suspensions suggests an overall decreased cell uptake of the dye. The microscopy and fluorimetry detected early events in the reaction of lymphocytes from tumour-bearing rats with the target tumour cells. It would appear that the changes in intracellular behaviour of the dye and in overall cell uptake after immune stimulation are a reflection of dissociated variations in internal and external cell membrane permeability, and may provide simple general means for recognizing cellular immune reactions.", "contents": "Acridine orange fluorescence cytochemistry for detecting lymphocyte immunoreactivity. Acridine orange staining reveals changes within 3 hours of in vitro stimulation of normal rat lymphocytes with mitogens, and of immune rat lymphocytes with the sensitizing antigen. An increased number of red fluorescent cytoplasmic organelles, presumably lysosomes are seen by fluorescence microscopy. Fluorimetry of the supernatants from stained cell suspensions suggests an overall decreased cell uptake of the dye. The microscopy and fluorimetry detected early events in the reaction of lymphocytes from tumour-bearing rats with the target tumour cells. It would appear that the changes in intracellular behaviour of the dye and in overall cell uptake after immune stimulation are a reflection of dissociated variations in internal and external cell membrane permeability, and may provide simple general means for recognizing cellular immune reactions.", "PMID": 965732}
{"id": "pubmed23n0032_6454", "title": "Rapid, safe and simple method for grafting whole thymus in the mouse.", "content": "A rapid, safe and simple method is presented for obtaining and grafting whole neonatal thymuses in mice. No anesthesia, sutures or incisions are required for implantation. The grafting technique involves no special skills except the ability to inject mice subcutaneously. Thymuses grafted by the method described show normal architecture, survive lethal irradiation and bone marrow reconstitution, repopulate thymic dependent areas of peripheral lymphoid organs and restore normal inflammatory cell responses to thymic deprived mice.", "contents": "Rapid, safe and simple method for grafting whole thymus in the mouse. A rapid, safe and simple method is presented for obtaining and grafting whole neonatal thymuses in mice. No anesthesia, sutures or incisions are required for implantation. The grafting technique involves no special skills except the ability to inject mice subcutaneously. Thymuses grafted by the method described show normal architecture, survive lethal irradiation and bone marrow reconstitution, repopulate thymic dependent areas of peripheral lymphoid organs and restore normal inflammatory cell responses to thymic deprived mice.", "PMID": 965733}
{"id": "pubmed23n0032_6455", "title": "Enzymatic conversion of human group O red cells into Group B active cells by alpha-D-galactosyltransferases of sera and salivas from group B and its variant types.", "content": "Salivas from group B secretor or non-secretor, acting on O red cells in the presence of UDP-galactose, each converted them into B cells, which were agglutinated against anti-B human serum (1:512) at the titre of thirty-two-fold, while secretor or non-secretor group AB salivas converted O red cells into B active cells, which were agglutinated by anti-B human serum (1:512) at the titre of eight- to sixteen-fold. The results indicate that the alpha-galactosyltransferases which participate in the biosynthesis of group B substance are secreted in group B or AB salivas of both secretor and non-secretor types as well as in their sera. Agglutinabilities of enzymatically converted B-active red cells against anti-B human serum indicate that alpha-galactosyltransferase activities of both serum and saliva from a weak B (Bw) individual, who has weak B antigens in red cells and saliva, were lower than those of normal group B. The alpha-galactosyltransferase activities in group Bm sera were lower than those of normal group B, while the enzyme activities in salivas of group Bm were demonstrated to the same degree in normal group B salivas.", "contents": "Enzymatic conversion of human group O red cells into Group B active cells by alpha-D-galactosyltransferases of sera and salivas from group B and its variant types. Salivas from group B secretor or non-secretor, acting on O red cells in the presence of UDP-galactose, each converted them into B cells, which were agglutinated against anti-B human serum (1:512) at the titre of thirty-two-fold, while secretor or non-secretor group AB salivas converted O red cells into B active cells, which were agglutinated by anti-B human serum (1:512) at the titre of eight- to sixteen-fold. The results indicate that the alpha-galactosyltransferases which participate in the biosynthesis of group B substance are secreted in group B or AB salivas of both secretor and non-secretor types as well as in their sera. Agglutinabilities of enzymatically converted B-active red cells against anti-B human serum indicate that alpha-galactosyltransferase activities of both serum and saliva from a weak B (Bw) individual, who has weak B antigens in red cells and saliva, were lower than those of normal group B. The alpha-galactosyltransferase activities in group Bm sera were lower than those of normal group B, while the enzyme activities in salivas of group Bm were demonstrated to the same degree in normal group B salivas.", "PMID": 965765}
{"id": "pubmed23n0032_6456", "title": "Anti-B activity of a lectin coexistent with blood group H-like substance in seeds of Euonymus sieboldiana.", "content": "Euonymus Sieboldiana seeds possess a lectin for human erythrocytes with anti-B specificity. Lectin fractions with strong agglutinating activity were separated by ammonium sulphate precipitation from blood group H-like substance, which is coexistent in the same seeds. The lectin has properties resembling a complete agglutinin, being non-dialysable, inactivated by heat treatment at 70 degrees C and specific for D-galactoside.", "contents": "Anti-B activity of a lectin coexistent with blood group H-like substance in seeds of Euonymus sieboldiana. Euonymus Sieboldiana seeds possess a lectin for human erythrocytes with anti-B specificity. Lectin fractions with strong agglutinating activity were separated by ammonium sulphate precipitation from blood group H-like substance, which is coexistent in the same seeds. The lectin has properties resembling a complete agglutinin, being non-dialysable, inactivated by heat treatment at 70 degrees C and specific for D-galactoside.", "PMID": 965766}
{"id": "pubmed23n0032_6457", "title": "Linkage relationships of the loci of the major histocompatibility complex in families with a recombination in the HLA region.", "content": "A number of families with an established recombination in the major histocompatibility complex has been investigated for markers known to be coded by genes of this linkage group. The results provide further data on the relative position of the loci for HLA-A, HLA-B, HLA-C, HLA-D, Bf, Chido, Rodgers and PGM3 on chromosome 6. A positive lodscore for linkage between HLA and blood group P was found; lodscores between HLA and nineteen other markers were negative.", "contents": "Linkage relationships of the loci of the major histocompatibility complex in families with a recombination in the HLA region. A number of families with an established recombination in the major histocompatibility complex has been investigated for markers known to be coded by genes of this linkage group. The results provide further data on the relative position of the loci for HLA-A, HLA-B, HLA-C, HLA-D, Bf, Chido, Rodgers and PGM3 on chromosome 6. A positive lodscore for linkage between HLA and blood group P was found; lodscores between HLA and nineteen other markers were negative.", "PMID": 965767}
{"id": "pubmed23n0032_6458", "title": "Genetic control of the immune response to ferritin in mice.", "content": "The immune response to the antigen horse spleen ferritin was tested in sixteen inbred strains of mice. Using a fixed antigen percentage  bound isotope technique, it was found that the quantity of antibody produced was related to the genetic status of the responding animal. A continuous distribution of responses was obtained, which were found to be linked to H-2 loci and the 2 non-H-2 loci 'Tla' and 'Theta' and these responses are detectable following primary immunization. It is suggested that the continuous distribution of quantitative antibody responses to ferritin, is compatible either with a large number of IR-genes or with a cross tolerance hypothesis requiring no IR-genes at all.", "contents": "Genetic control of the immune response to ferritin in mice. The immune response to the antigen horse spleen ferritin was tested in sixteen inbred strains of mice. Using a fixed antigen percentage  bound isotope technique, it was found that the quantity of antibody produced was related to the genetic status of the responding animal. A continuous distribution of responses was obtained, which were found to be linked to H-2 loci and the 2 non-H-2 loci 'Tla' and 'Theta' and these responses are detectable following primary immunization. It is suggested that the continuous distribution of quantitative antibody responses to ferritin, is compatible either with a large number of IR-genes or with a cross tolerance hypothesis requiring no IR-genes at all.", "PMID": 965768}
{"id": "pubmed23n0032_6459", "title": "Short communication structure of 'natural antibodies' in lower vertebrates.", "content": "'Natural' antibodies are generally believed to possess solely 2H-2L immunoglobulin structure. They have many disulphide bonds, the structural importance of which is not yet understood. However, some natural heterohaemagglutinins in the sera of some lower vertebrates (eel, shark, perhaps also trout and toad) are subunit proteins; the subunits are held together not by disulphide bonds but solely by non-covalent interactions like animal lectins. The relevance of these findings for immunophylogeny is discussed.", "contents": "Short communication structure of 'natural antibodies' in lower vertebrates. 'Natural' antibodies are generally believed to possess solely 2H-2L immunoglobulin structure. They have many disulphide bonds, the structural importance of which is not yet understood. However, some natural heterohaemagglutinins in the sera of some lower vertebrates (eel, shark, perhaps also trout and toad) are subunit proteins; the subunits are held together not by disulphide bonds but solely by non-covalent interactions like animal lectins. The relevance of these findings for immunophylogeny is discussed.", "PMID": 965769}
{"id": "pubmed23n0032_6460", "title": "Incidence of a weak HL-A W27 antigen in a Czech family.", "content": "In two members of a Czech family a weak W 27 antigen was found. The lymphocytes of the two individuals showed an identical positive cytotoxic reaction only with some sera having anti-W 27 specificity; anti-W 27 of both the said sera and those showing negative cytotoxic test was absorbed by the lymphocytes. The thrombocyte absorption capacity was substantially poorer.", "contents": "Incidence of a weak HL-A W27 antigen in a Czech family. In two members of a Czech family a weak W 27 antigen was found. The lymphocytes of the two individuals showed an identical positive cytotoxic reaction only with some sera having anti-W 27 specificity; anti-W 27 of both the said sera and those showing negative cytotoxic test was absorbed by the lymphocytes. The thrombocyte absorption capacity was substantially poorer.", "PMID": 965770}
{"id": "pubmed23n0032_6461", "title": "Morphology of delayed-type hypersensitivity reactions in man.", "content": "The new morphologic findings reviewed here substantially alter prevalent conceptions of delayed hypersensitivity as a simple cutaneous infiltration of lymphocytes and macrophages. By assigning an integral role of basophils, mast cells, the microvasculature, and the clotting system, the findings have far-reaching implications for an understanding of these clinically important reactions. Morphologic observations, of course, represent only a first step, a foundation on which subsequent immunologic, physiologic, and biochemical experiments can build. Much further work will be required to interrelate these new findings and to integrate them with older observations into a coherent sequence of events which can explain the pathogenesis of cell-mediated reactions. A preliminary attempt in this direction, based on present, rather incomplete information, is presented in Figure 8 as a basis for further investigation.", "contents": "Morphology of delayed-type hypersensitivity reactions in man. The new morphologic findings reviewed here substantially alter prevalent conceptions of delayed hypersensitivity as a simple cutaneous infiltration of lymphocytes and macrophages. By assigning an integral role of basophils, mast cells, the microvasculature, and the clotting system, the findings have far-reaching implications for an understanding of these clinically important reactions. Morphologic observations, of course, represent only a first step, a foundation on which subsequent immunologic, physiologic, and biochemical experiments can build. Much further work will be required to interrelate these new findings and to integrate them with older observations into a coherent sequence of events which can explain the pathogenesis of cell-mediated reactions. A preliminary attempt in this direction, based on present, rather incomplete information, is presented in Figure 8 as a basis for further investigation.", "PMID": 965787}
{"id": "pubmed23n0032_6462", "title": "Erythropoiesis in chronic renal disease.", "content": "The diminished erythropoiesis in the anemia of chronic renal disease has been attributed to three possible factors: (1) decreased erythropoietin production, (2) inhibition of erythropoietin activity, and (3) decreased bone marrow response to erythropoietin. In this report we isolated and evaluated these parameters in 19 patients with chronic renal disease, nine patients with iron-deficiency anemia, and seven control subjects. The results in patients with chronic renal failure were as follows: (1) erythropoietin enhanced heme synthesis in bone marrow cell cultures by 88 +/- 12 per cent in renal failure, as compared to 65 +/- 7 per cent in the control group; (2) plasma erythropoietin activity did not increase appropriately for the degree of anemia; and (3) erythropoietin inhibitor activity in renal failure was not greater than in a control group. In conclusion, the relative failure of erythropoiesis in chronic renal disease appears to be due primarily to decreased production of erythropoietin and not to diminished marrow response to erythropoietin.", "contents": "Erythropoiesis in chronic renal disease. The diminished erythropoiesis in the anemia of chronic renal disease has been attributed to three possible factors: (1) decreased erythropoietin production, (2) inhibition of erythropoietin activity, and (3) decreased bone marrow response to erythropoietin. In this report we isolated and evaluated these parameters in 19 patients with chronic renal disease, nine patients with iron-deficiency anemia, and seven control subjects. The results in patients with chronic renal failure were as follows: (1) erythropoietin enhanced heme synthesis in bone marrow cell cultures by 88 +/- 12 per cent in renal failure, as compared to 65 +/- 7 per cent in the control group; (2) plasma erythropoietin activity did not increase appropriately for the degree of anemia; and (3) erythropoietin inhibitor activity in renal failure was not greater than in a control group. In conclusion, the relative failure of erythropoiesis in chronic renal disease appears to be due primarily to decreased production of erythropoietin and not to diminished marrow response to erythropoietin.", "PMID": 965807}
{"id": "pubmed23n0032_6463", "title": "Coagulation factor XIII concentration in sickle-cell disease.", "content": "Plasma factor XIII was assayed serially for a 1 1/2 to 2 year period in four patients with sickle-cell disease (SSD), during which several episodes of SSD crisis were observed. During crisis-free periods, plasma factor XIII concentration was lower than in normal subjects. With crisis onset, there was usually a concomitant fall in platelets and in plasma factor XIII soon after followed by a rise in fibrinogen concentration and platelet count. Following crisis resolution there was an increase in plasma factor XIII concentration peaking 3 weeks after crisis onset and occurring at the time of subsidence of thrombocytosis. It is suggested that the observed changes in plasma factor XIII concentration in SSD patients support the hypothesis that in the early stage of SSD crisis, fibrin deposition and/or thrombosis significantly enhance the role of sickled erythrocytes in producing vascular occlusion and/or organ infarction.", "contents": "Coagulation factor XIII concentration in sickle-cell disease. Plasma factor XIII was assayed serially for a 1 1/2 to 2 year period in four patients with sickle-cell disease (SSD), during which several episodes of SSD crisis were observed. During crisis-free periods, plasma factor XIII concentration was lower than in normal subjects. With crisis onset, there was usually a concomitant fall in platelets and in plasma factor XIII soon after followed by a rise in fibrinogen concentration and platelet count. Following crisis resolution there was an increase in plasma factor XIII concentration peaking 3 weeks after crisis onset and occurring at the time of subsidence of thrombocytosis. It is suggested that the observed changes in plasma factor XIII concentration in SSD patients support the hypothesis that in the early stage of SSD crisis, fibrin deposition and/or thrombosis significantly enhance the role of sickled erythrocytes in producing vascular occlusion and/or organ infarction.", "PMID": 965808}
{"id": "pubmed23n0032_6464", "title": "The erythropoietin response to the anemia of thermal injury.", "content": "Erythropoietin excretion was persistently increased following major thermal injury in 4 of 5 patients. A good correlation was found between erythropoietin excretion and red cell mass but not between erythropoietin excretion and hematocrit. In spite of the increased erythropoietin, erythropoiesis in these thermally injured patients was inadequate to compensate for erythrocyte deficits as judged by bone marrow morphology, reticulocyte counts, and transfusion requirements.", "contents": "The erythropoietin response to the anemia of thermal injury. Erythropoietin excretion was persistently increased following major thermal injury in 4 of 5 patients. A good correlation was found between erythropoietin excretion and red cell mass but not between erythropoietin excretion and hematocrit. In spite of the increased erythropoietin, erythropoiesis in these thermally injured patients was inadequate to compensate for erythrocyte deficits as judged by bone marrow morphology, reticulocyte counts, and transfusion requirements.", "PMID": 965809}
{"id": "pubmed23n0032_6465", "title": "Effects of ultrafiltration on solute clearances in hollow fiber artificial kidneys.", "content": "Although solute clearances in artificial kidney coils increase with ultrafiltration (UF), we have previously shown that increases are usually less than UF rate (most likely because of decreases in diffusive transport with UF coils and, for larger solutes, molecular sieving). The present studies demonstrate the effects of UF on clearances of Na and bromsulphalein (BSP) (mol. wt. 838) in hollow fiber dialyzers. Clearances were measured at increasing transmembrane hydrostatic pressures at perfusion rates of 200 and 500 ml. per minute. Fractions of total clearance attributable to diffusion as compared to solvent drag forces were calculated. Sieving coefficients were determined in studies where diffusion was minimized and clearance was primarily by solvent drag. Clearance increases were less than UF rate only for BSP; molecular sieving most likely accounts for the difference at high perfusion rates. Only at 200 ml. per minute was slight decrease of diffusion with UF suggested. Thus, in contrast to coils, there is minimal or no decrease in diffusion with UF in hollow fiber dialyzers.", "contents": "Effects of ultrafiltration on solute clearances in hollow fiber artificial kidneys. Although solute clearances in artificial kidney coils increase with ultrafiltration (UF), we have previously shown that increases are usually less than UF rate (most likely because of decreases in diffusive transport with UF coils and, for larger solutes, molecular sieving). The present studies demonstrate the effects of UF on clearances of Na and bromsulphalein (BSP) (mol. wt. 838) in hollow fiber dialyzers. Clearances were measured at increasing transmembrane hydrostatic pressures at perfusion rates of 200 and 500 ml. per minute. Fractions of total clearance attributable to diffusion as compared to solvent drag forces were calculated. Sieving coefficients were determined in studies where diffusion was minimized and clearance was primarily by solvent drag. Clearance increases were less than UF rate only for BSP; molecular sieving most likely accounts for the difference at high perfusion rates. Only at 200 ml. per minute was slight decrease of diffusion with UF suggested. Thus, in contrast to coils, there is minimal or no decrease in diffusion with UF in hollow fiber dialyzers.", "PMID": 965810}
{"id": "pubmed23n0032_6466", "title": "Evidence for cell-mediated immunity to collagen in progressive systemic sclerosis.", "content": "Peripheral blood leukocytes from 12 patients with progressive systemic sclerosis and from 12 normal subjects were cultured in the presence of human skin collagen, lathryritic chick skin collagen, and alpha-chains from chick skin collagen. Leukocytes from 11 of the patients produced a factor chemotactic for human monocytes when cultured with the collagen preparations. The one patient whose cells did not respond to the collagen preparations also failed to respond to other common antigens and was apparently anergic. In addition, lymphocytes from three of the patients were found to transform when cultured with the collagen preparations. These responses were significantly different from the response of leukocytes from normal control subjects. The data suggest that patients with progressive systemic sclerosis may have cellular immunity to collagen.", "contents": "Evidence for cell-mediated immunity to collagen in progressive systemic sclerosis. Peripheral blood leukocytes from 12 patients with progressive systemic sclerosis and from 12 normal subjects were cultured in the presence of human skin collagen, lathryritic chick skin collagen, and alpha-chains from chick skin collagen. Leukocytes from 11 of the patients produced a factor chemotactic for human monocytes when cultured with the collagen preparations. The one patient whose cells did not respond to the collagen preparations also failed to respond to other common antigens and was apparently anergic. In addition, lymphocytes from three of the patients were found to transform when cultured with the collagen preparations. These responses were significantly different from the response of leukocytes from normal control subjects. The data suggest that patients with progressive systemic sclerosis may have cellular immunity to collagen.", "PMID": 965811}
{"id": "pubmed23n0032_6467", "title": "A simple and precise method of determining true sodium, potassium, and chloride concentrations in hyperlipemia.", "content": "Sodium, potassium, and chloride levels are artifactually depressed in hyperlipemic sera. Accurate electrolyte levels are needed for management of patients with hyperlipemia, but present methods for correcting the values (serum water and/or osmolality determinations) either are technically cumbersome or fail to provide accurate data to correct the falsely low levels. Alternatively, to determine true sodium, potassium, and chloride concentrations in hyperlipemic sera, only the required electrolyte values and the triglycerides are measured. The percentage by which the measured electrolyte levels in the hyperlipemic sample must be increased to approximate the true values is given by the following equation: per cent increase = 2.1 X triglycerides (Gm./dl.) - 0.6.", "contents": "A simple and precise method of determining true sodium, potassium, and chloride concentrations in hyperlipemia. Sodium, potassium, and chloride levels are artifactually depressed in hyperlipemic sera. Accurate electrolyte levels are needed for management of patients with hyperlipemia, but present methods for correcting the values (serum water and/or osmolality determinations) either are technically cumbersome or fail to provide accurate data to correct the falsely low levels. Alternatively, to determine true sodium, potassium, and chloride concentrations in hyperlipemic sera, only the required electrolyte values and the triglycerides are measured. The percentage by which the measured electrolyte levels in the hyperlipemic sample must be increased to approximate the true values is given by the following equation: per cent increase = 2.1 X triglycerides (Gm./dl.) - 0.6.", "PMID": 965812}
{"id": "pubmed23n0032_6468", "title": "Meni\u00e8re's disease in childhood.", "content": "Two cases of Meni\u00e8re's disease in childhood were found during one year from 850 examinations of children. The clinical and audiological findings are similar to those seen in adults.", "contents": "Meni\u00e8re's disease in childhood. Two cases of Meni\u00e8re's disease in childhood were found during one year from 850 examinations of children. The clinical and audiological findings are similar to those seen in adults.", "PMID": 965819}
{"id": "pubmed23n0032_6469", "title": "Vestibular nerve section for M\u00e9ni\u00e8re's disease.", "content": "In summary: (i) the fact that VNS spares auditory function makes it preferable to labyrinthectomy. A comparison of VNS and the \"conservative\" operations on the inner ear shown that they have much the same effect on hearing. VNS by controlling vertigo may also provide an additional benefit by eliminating one of the stress factors concerned in the perpetuation of hydrops; (2) in the treatment of vertigo, VNS is now the most effective method of treatment. Because of elimination of vertigo is what chiefly concerns most of our patients, we suggest that when medical treatment fails to control vertigo, VNS is the surgical method of choice.", "contents": "Vestibular nerve section for M\u00e9ni\u00e8re's disease. In summary: (i) the fact that VNS spares auditory function makes it preferable to labyrinthectomy. A comparison of VNS and the \"conservative\" operations on the inner ear shown that they have much the same effect on hearing. VNS by controlling vertigo may also provide an additional benefit by eliminating one of the stress factors concerned in the perpetuation of hydrops; (2) in the treatment of vertigo, VNS is now the most effective method of treatment. Because of elimination of vertigo is what chiefly concerns most of our patients, we suggest that when medical treatment fails to control vertigo, VNS is the surgical method of choice.", "PMID": 965820}
{"id": "pubmed23n0032_6470", "title": "Destruction of the endolymphatic sac in the cat.", "content": "The endolymphatic duct and sac were destroyed by drilling in a cat that was sacrificied after 2 3/4 years. Histological examination of the temporal bones showed marked endolymphatic hydrops; degeneration of the stria vascularis in the upper coil; degeneration of the organ of Corti and spiral ganglion in the upper and middle coils; and degeneration of the macula of the saccule. It is probable that, in some cases at least, the primary pathology of Meni\u00e8re's disease is in the endolymphatic sac.", "contents": "Destruction of the endolymphatic sac in the cat. The endolymphatic duct and sac were destroyed by drilling in a cat that was sacrificied after 2 3/4 years. Histological examination of the temporal bones showed marked endolymphatic hydrops; degeneration of the stria vascularis in the upper coil; degeneration of the organ of Corti and spiral ganglion in the upper and middle coils; and degeneration of the macula of the saccule. It is probable that, in some cases at least, the primary pathology of Meni\u00e8re's disease is in the endolymphatic sac.", "PMID": 965821}
{"id": "pubmed23n0032_6471", "title": "The tympanic membrane/ossicular chain Block implant - early impression.", "content": "Twenty-six tympanic membrane-ossicular chain implants have been used during a twelve-month period in the reconstruction of middle ears which had been affected by tympanosclerosis, inflammatory processes and cholesteatoma. The results which have been achieved, both from the point of view of anatomy and function, are presented and changes observed in the post-operative period are described. It is suggested that alternative methods of preservation of these grafts and modifications in technique may give more consistent results.", "contents": "The tympanic membrane/ossicular chain Block implant - early impression. Twenty-six tympanic membrane-ossicular chain implants have been used during a twelve-month period in the reconstruction of middle ears which had been affected by tympanosclerosis, inflammatory processes and cholesteatoma. The results which have been achieved, both from the point of view of anatomy and function, are presented and changes observed in the post-operative period are described. It is suggested that alternative methods of preservation of these grafts and modifications in technique may give more consistent results.", "PMID": 965822}
{"id": "pubmed23n0032_6472", "title": "Rhinoentomophthoromycosis.", "content": "A rare case of Rhinophycomycosis entomophthora treated with a combination of Bacterium, potassium iodide and steroid is reported. Clinical features, epidemiology, mycology, and management are discussed.", "contents": "Rhinoentomophthoromycosis. A rare case of Rhinophycomycosis entomophthora treated with a combination of Bacterium, potassium iodide and steroid is reported. Clinical features, epidemiology, mycology, and management are discussed.", "PMID": 965825}
{"id": "pubmed23n0032_6473", "title": "E.N.T. complications in thalassaemia major.", "content": "Twenty-six cases of thalassaemia major have been investigated for E.N.T. complications. Twelve (46 percent) had significantly problems requiring surgery. Since the prognosis of the disease is improving dramatically, these patients should not be denied treatment. bony hypertrophy of the turbinates was a noticeable feature in the untreated group and does not seem to have been described before.", "contents": "E.N.T. complications in thalassaemia major. Twenty-six cases of thalassaemia major have been investigated for E.N.T. complications. Twelve (46 percent) had significantly problems requiring surgery. Since the prognosis of the disease is improving dramatically, these patients should not be denied treatment. bony hypertrophy of the turbinates was a noticeable feature in the untreated group and does not seem to have been described before.", "PMID": 965826}
{"id": "pubmed23n0032_6474", "title": "Failure to thrive.", "content": "We report a case of a \"butterfly\" nut removed successfully from the oesophagus of a 1-year-old baby. This foreign body was thought to have been present for three months. Continuous cough, feeding difficulty and weight loss were the predominant clinical features. The value of a chest X-ray is demonstrated.", "contents": "Failure to thrive. We report a case of a \"butterfly\" nut removed successfully from the oesophagus of a 1-year-old baby. This foreign body was thought to have been present for three months. Continuous cough, feeding difficulty and weight loss were the predominant clinical features. The value of a chest X-ray is demonstrated.", "PMID": 965827}
{"id": "pubmed23n0032_6475", "title": "The absolute rate of fatty acid synthesis by mammary gland slices from lactating rats.", "content": "Mammary gland utilizes a combination of substrates in vivo. To determine if and how the substrates presented to the gland alter milk fat synthesis, slices of mammary gland of lactating rats were incubated with combinations of glucose, L-lactate, and pyruvate. Uptakes and conversions of uniformly and specifically labeled substrates to CO2 and fatty acid were measured. The absolute rate of fatty acid synthesis was measured by incoporation of tritium from tritiated water into fatty acid. The extent, but not the type, of fatty acids synthesized was affected by the substrates utilized. Glucose stimulated uptake and conversion to fatty acid of l-lactate and, to a smaller extent, of pyruvate. Analysis of CO2 and fatty acid yields revealed that (a) the major stimulatory effect of glucose was on the conversion of acetyl coenyzme A from other substrates to fatty acid; (b) the rate of fatty acid synthesis paralleled the activity of the hexose monophosphate pathway up to a point. A likely source of the NADPH required beyond this point is provided by oxidation of malate to pyruvate. Reduction of NADH of oxaloacetate, produced during citrate cleavage, would yield malate. Hence, maximal synthesis would require production of NADPH and NADH, a condition met when glucose and L-lactate were substrates. Decreased fatty acids synthesis in pyruvate's presence supports this suggested requirement for cytosolic NADH in rat mammary gland.", "contents": "The absolute rate of fatty acid synthesis by mammary gland slices from lactating rats. Mammary gland utilizes a combination of substrates in vivo. To determine if and how the substrates presented to the gland alter milk fat synthesis, slices of mammary gland of lactating rats were incubated with combinations of glucose, L-lactate, and pyruvate. Uptakes and conversions of uniformly and specifically labeled substrates to CO2 and fatty acid were measured. The absolute rate of fatty acid synthesis was measured by incoporation of tritium from tritiated water into fatty acid. The extent, but not the type, of fatty acids synthesized was affected by the substrates utilized. Glucose stimulated uptake and conversion to fatty acid of l-lactate and, to a smaller extent, of pyruvate. Analysis of CO2 and fatty acid yields revealed that (a) the major stimulatory effect of glucose was on the conversion of acetyl coenyzme A from other substrates to fatty acid; (b) the rate of fatty acid synthesis paralleled the activity of the hexose monophosphate pathway up to a point. A likely source of the NADPH required beyond this point is provided by oxidation of malate to pyruvate. Reduction of NADH of oxaloacetate, produced during citrate cleavage, would yield malate. Hence, maximal synthesis would require production of NADPH and NADH, a condition met when glucose and L-lactate were substrates. Decreased fatty acids synthesis in pyruvate's presence supports this suggested requirement for cytosolic NADH in rat mammary gland.", "PMID": 965839}
{"id": "pubmed23n0032_6476", "title": "C26-Analogs of naturally occurring C27 bile alcohols.", "content": "C26 Bile of the 24-nor-5beta-cholestane series were prepared starting from methyl cholate. A Grignard reaction of methyl magnesium iodide with methyl cholate yielded 24-nor-5beta-cholestane-3alpha, 7alpha, 12alpha, 25-tetrol which was dehydrated TO FORM a mixture of 24-nor-5beta-cholest-23-ene-3alpha, 7alpha, 12 alpha-triol and the corresponding delta25 compound. Oxidation of the former with OsO4 yielded 24-nor-5beta-cholestane-3alpha, 7alpha, 12alpha, 23epsilon, 25pentol, while catalytic hydrogenation of a mixture of the delta23 and delta25 triols resulted in the formation of 24-nor-5beta-cholestane-3-alpha-, 7alpha-12alpha-triol. The structures of these new compounds were confirmed by infrared and nuclear megnetic resonance spectrometry and by gas-liquid chromatography-mass spectrometry.", "contents": "C26-Analogs of naturally occurring C27 bile alcohols. C26 Bile of the 24-nor-5beta-cholestane series were prepared starting from methyl cholate. A Grignard reaction of methyl magnesium iodide with methyl cholate yielded 24-nor-5beta-cholestane-3alpha, 7alpha, 12alpha, 25-tetrol which was dehydrated TO FORM a mixture of 24-nor-5beta-cholest-23-ene-3alpha, 7alpha, 12 alpha-triol and the corresponding delta25 compound. Oxidation of the former with OsO4 yielded 24-nor-5beta-cholestane-3alpha, 7alpha, 12alpha, 23epsilon, 25pentol, while catalytic hydrogenation of a mixture of the delta23 and delta25 triols resulted in the formation of 24-nor-5beta-cholestane-3-alpha-, 7alpha-12alpha-triol. The structures of these new compounds were confirmed by infrared and nuclear megnetic resonance spectrometry and by gas-liquid chromatography-mass spectrometry.", "PMID": 965840}
{"id": "pubmed23n0032_6477", "title": "Interactions of pancreatic lipase with bile salts and dodecyl sulfate.", "content": "Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the formation of mixed bile salt-SDS micelles.", "contents": "Interactions of pancreatic lipase with bile salts and dodecyl sulfate. Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the formation of mixed bile salt-SDS micelles.", "PMID": 965841}
{"id": "pubmed23n0032_6478", "title": "Factors affecting fatty acid oxidation in fat cells isolated from rat white adipose tissue.", "content": "Fat cells isolated from rat epididymal adipose tissue were incubated with albumin-bound [14C]palmitate. Incorporation of 14C into 14CO2 and glycerides was measured. Some evidence is presented to suggest that the exogenous palmitate pool is in isotopic equilibrium with intracellular precursors for these metabolic processes. Precautions were taken to minimize dilution of the exogenous palmitate pool by fatty acids released from the cells. 14CO2 production from [1-14C]palmitate was 3 times that from [16-14C]palmitate. Octanoate increased this differential oxidation of palmitate carbons and also inhibited palmitate oxidation without similarly affecting esterification. Glucose increases palmitate esterification in cells from fed or starved rats. Insulin potentiated this effect of glucose. Glucose influenced palmitate oxidation in a more complex manner, dependent upon the glucose concentration. Both the observation that esterification constitutes 99% of the metabolic flux of fatty acid and the manner in which glucose, insulin, or starvation influence palmitate esterification and oxidation suggested that factors controlling esterification may alter oxidation as a secondary effect, but not vice versa. It is suggested that oxidation and esterification compete for a single intracellular precursor, possibly extramitochondrial long chain fatty acyl CoA.", "contents": "Factors affecting fatty acid oxidation in fat cells isolated from rat white adipose tissue. Fat cells isolated from rat epididymal adipose tissue were incubated with albumin-bound [14C]palmitate. Incorporation of 14C into 14CO2 and glycerides was measured. Some evidence is presented to suggest that the exogenous palmitate pool is in isotopic equilibrium with intracellular precursors for these metabolic processes. Precautions were taken to minimize dilution of the exogenous palmitate pool by fatty acids released from the cells. 14CO2 production from [1-14C]palmitate was 3 times that from [16-14C]palmitate. Octanoate increased this differential oxidation of palmitate carbons and also inhibited palmitate oxidation without similarly affecting esterification. Glucose increases palmitate esterification in cells from fed or starved rats. Insulin potentiated this effect of glucose. Glucose influenced palmitate oxidation in a more complex manner, dependent upon the glucose concentration. Both the observation that esterification constitutes 99% of the metabolic flux of fatty acid and the manner in which glucose, insulin, or starvation influence palmitate esterification and oxidation suggested that factors controlling esterification may alter oxidation as a secondary effect, but not vice versa. It is suggested that oxidation and esterification compete for a single intracellular precursor, possibly extramitochondrial long chain fatty acyl CoA.", "PMID": 965842}
{"id": "pubmed23n0032_6479", "title": "Equally rapid activation of lipogenesis in nibbling and gorging mice.", "content": "Minimal average rates of exogenous glucose-C-conversion to whole body, total lipid fatty acids were measured in nibbling and gorging mice. Gorgers trained to eat 1 meal/day (8-10 am) were fasted 22-24 hr and given [14C]glucose with pure glucose, 30% glucose in water, or a 58% glucose, fat-free diet. Conversion of glucose-C to total lipid fatty acids increased from 0.6 (fasted) to approximately 20 mug/min/20 g body weight during 40 min after glucose feeding using each test meal. Dietary amino acids were not required for activation of lipogenesis in gorgers. Exogenous glucose-C was incorporated into fatty acids as fast in nibbling mice as in gorgers. This was true after varying all of the following conditions: training period, number of meals gorged, previous fasting time, and diet composition. The total rate of fatty acid synthesis from body glucose-C during absorption of a glucose load was also estimated in absorption of a glucose load was also estimated in previously fasted nibbling and gorging mice. These estimates were based on composite, serial measurement of both plasma glucose specific activities and 14C-labeled fatty acids. The total rate of fatty acid synthesis from both exogenous and endogenous glucose-C was only 15% higher than the rate from exogenous glucose-C between 10 and 40 min. No significant differences between nibblers and gorgers were found.", "contents": "Equally rapid activation of lipogenesis in nibbling and gorging mice. Minimal average rates of exogenous glucose-C-conversion to whole body, total lipid fatty acids were measured in nibbling and gorging mice. Gorgers trained to eat 1 meal/day (8-10 am) were fasted 22-24 hr and given [14C]glucose with pure glucose, 30% glucose in water, or a 58% glucose, fat-free diet. Conversion of glucose-C to total lipid fatty acids increased from 0.6 (fasted) to approximately 20 mug/min/20 g body weight during 40 min after glucose feeding using each test meal. Dietary amino acids were not required for activation of lipogenesis in gorgers. Exogenous glucose-C was incorporated into fatty acids as fast in nibbling mice as in gorgers. This was true after varying all of the following conditions: training period, number of meals gorged, previous fasting time, and diet composition. The total rate of fatty acid synthesis from body glucose-C during absorption of a glucose load was also estimated in absorption of a glucose load was also estimated in previously fasted nibbling and gorging mice. These estimates were based on composite, serial measurement of both plasma glucose specific activities and 14C-labeled fatty acids. The total rate of fatty acid synthesis from both exogenous and endogenous glucose-C was only 15% higher than the rate from exogenous glucose-C between 10 and 40 min. No significant differences between nibblers and gorgers were found.", "PMID": 965843}
{"id": "pubmed23n0032_6480", "title": "Determination of protein in adipose tissue extracts.", "content": "An accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-increasing substance and the precipitate is extracted with an organic solvent. This interference caused by lipids, sulfhydryl reagents, and several other compounds is eliminated. The accuracy of the determination of dilute reference protein solutions (2-20 mug/ml) is demonstrated.", "contents": "Determination of protein in adipose tissue extracts. An accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-increasing substance and the precipitate is extracted with an organic solvent. This interference caused by lipids, sulfhydryl reagents, and several other compounds is eliminated. The accuracy of the determination of dilute reference protein solutions (2-20 mug/ml) is demonstrated.", "PMID": 965844}
{"id": "pubmed23n0032_6481", "title": "Silicic acid thin-layer chromatography of conjugated and free bile acids.", "content": "The separation of free bile acids, taurine conjugates, and glycine conjugates from one another was achieved by thin-layer chromatography, using a solvent system of isopropanol glacial acetic acid 93:7 in a solvent system of hexane-methylethylketone-glacial acetic acid 56:36:8 (v/v), it was possible to separate cholic, chenodeoxycholic, deoxycholic, and lithocholic acids. Application to a biological bile sample was demonstrated.", "contents": "Silicic acid thin-layer chromatography of conjugated and free bile acids. The separation of free bile acids, taurine conjugates, and glycine conjugates from one another was achieved by thin-layer chromatography, using a solvent system of isopropanol glacial acetic acid 93:7 in a solvent system of hexane-methylethylketone-glacial acetic acid 56:36:8 (v/v), it was possible to separate cholic, chenodeoxycholic, deoxycholic, and lithocholic acids. Application to a biological bile sample was demonstrated.", "PMID": 965845}
{"id": "pubmed23n0032_6482", "title": "Prolactin, diuretics and urinary electrolytes in normal subjects.", "content": "The relationship of plasma prolactin concentration and renal electrolyte excretion has been investigated in six normal male volunteers. In two studies, 80 mg frusemide were administered at 18.00 h on Day 1 and followed by dietary sodium restriction. In study A, after 38 h of sodium depletion, a second dose of frusemide was administered at 08.00 h on Day 3. In study B, after 14 h of sodium depletion, the effect of administration of 100 mg spironolactone or 45 mg prorenoate potassium (another aldosterone antagonist) at 08.00 h on Day 2 was compared with that of a placebo. After the first dose of frusemide in study A, the mean plasma prolactin concentration correlated negatively with the urinary Na and K excretion over 5 h. After 38 h sodium depletion, the plasma prolactin concentration correlated positively with urinary Na excretion following the second dose of frusemide. In study B, after Na depletion for 14 h the plasma prolactin concentration of 08.00 h on Day 2 had a positive correlation with the 24 h urinary log10 Na:K ratio following placebo administration and had negative correlations with the true urinary log10 Na:K ratio following spironolactone and prorenoate potassium administration. Neither acute Na deprivation nor the administration of single doses of frusemide, spironolactone or proprenoate potassium appeared to affect the normal circadian rhythm of plasma prolactin concentrations which remained constant for each subject throughout the 3 months covered by the investigation. The correlations of plasma prolactin concentration to renal excretion of electrolytes, with no evidence for a negative feedback control mechanism, suggest an indirect relationship between prolactin and renal function.", "contents": "Prolactin, diuretics and urinary electrolytes in normal subjects. The relationship of plasma prolactin concentration and renal electrolyte excretion has been investigated in six normal male volunteers. In two studies, 80 mg frusemide were administered at 18.00 h on Day 1 and followed by dietary sodium restriction. In study A, after 38 h of sodium depletion, a second dose of frusemide was administered at 08.00 h on Day 3. In study B, after 14 h of sodium depletion, the effect of administration of 100 mg spironolactone or 45 mg prorenoate potassium (another aldosterone antagonist) at 08.00 h on Day 2 was compared with that of a placebo. After the first dose of frusemide in study A, the mean plasma prolactin concentration correlated negatively with the urinary Na and K excretion over 5 h. After 38 h sodium depletion, the plasma prolactin concentration correlated positively with urinary Na excretion following the second dose of frusemide. In study B, after Na depletion for 14 h the plasma prolactin concentration of 08.00 h on Day 2 had a positive correlation with the 24 h urinary log10 Na:K ratio following placebo administration and had negative correlations with the true urinary log10 Na:K ratio following spironolactone and prorenoate potassium administration. Neither acute Na deprivation nor the administration of single doses of frusemide, spironolactone or proprenoate potassium appeared to affect the normal circadian rhythm of plasma prolactin concentrations which remained constant for each subject throughout the 3 months covered by the investigation. The correlations of plasma prolactin concentration to renal excretion of electrolytes, with no evidence for a negative feedback control mechanism, suggest an indirect relationship between prolactin and renal function.", "PMID": 965879}
{"id": "pubmed23n0032_6483", "title": "Ageing and ovarian delta 5-3 beta-hydrosteroid dehydrogenase in the pregnant mouse.", "content": "Aged (12- to 14-month-old) C57BL oestrous mice exhibited significantly lower (P less than 0-001) ovarian delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) concentration, specific activity and total content than young (3-month-old) oestrous mice, suggesting a decrease in the potential of the older animals to produce ovarian delta 4-3-oxosteroids. Mice in both age groups maintaining pregnancy to 10 or 18 days post coitum (p.c.) had similar values for activity of ovarian 3 beta-HSD. In the aged females in which foetal resorption had occurred, the majority of foetuses had been resorbed by 10 days p.c. However, ovarian 3 beta-HSD activity in these animals was not significantly different from that of young or aged mice maintaining pregnancy. By day 18, however, ovarian dehydrogenase activity in aged females failing to maintain pregnancy had decreased significantly. It is suggested that foetal death in aged mice is not the result of a deficiency in ovarian 3 beta-HSD, but rather may initiate luteal regression and consequently a decline in 3 beta-HSD.", "contents": "Ageing and ovarian delta 5-3 beta-hydrosteroid dehydrogenase in the pregnant mouse. Aged (12- to 14-month-old) C57BL oestrous mice exhibited significantly lower (P less than 0-001) ovarian delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) concentration, specific activity and total content than young (3-month-old) oestrous mice, suggesting a decrease in the potential of the older animals to produce ovarian delta 4-3-oxosteroids. Mice in both age groups maintaining pregnancy to 10 or 18 days post coitum (p.c.) had similar values for activity of ovarian 3 beta-HSD. In the aged females in which foetal resorption had occurred, the majority of foetuses had been resorbed by 10 days p.c. However, ovarian 3 beta-HSD activity in these animals was not significantly different from that of young or aged mice maintaining pregnancy. By day 18, however, ovarian dehydrogenase activity in aged females failing to maintain pregnancy had decreased significantly. It is suggested that foetal death in aged mice is not the result of a deficiency in ovarian 3 beta-HSD, but rather may initiate luteal regression and consequently a decline in 3 beta-HSD.", "PMID": 965880}
{"id": "pubmed23n0032_6484", "title": "Plasma and adrenal cortisol concentrations in foetal, newborn and mother guinea-pigs during the perinatal period.", "content": "Concentrations of cortisol, corticosterone and cortisone in the plasma and adrenal glands, liver glycogen and plasma glucose of foetal, newborn and mother guinea-pigs were estimated during the last 6 days of pregnancy and throughout the first 24 h post partum. At the same time progesterone was measured in the plasma of the mother. During the prepartum rise in foetal plasma cortisol levels and liver glycogen, no significant change in the foetal adrenal cortisol content was observed. The plasma and adrenal cortisol concentrations of the mother were much higher than those observed in the foetus and increased significantly before parturition. In the mother as in the foetus, cortisone and corticosterone represent only a small percentage of corticosteroids compared with cortisol. These results indicate that the autonomous capacity of foetal adrenals, inhibited by maternal secretions before term, appears suddenly at birth.", "contents": "Plasma and adrenal cortisol concentrations in foetal, newborn and mother guinea-pigs during the perinatal period. Concentrations of cortisol, corticosterone and cortisone in the plasma and adrenal glands, liver glycogen and plasma glucose of foetal, newborn and mother guinea-pigs were estimated during the last 6 days of pregnancy and throughout the first 24 h post partum. At the same time progesterone was measured in the plasma of the mother. During the prepartum rise in foetal plasma cortisol levels and liver glycogen, no significant change in the foetal adrenal cortisol content was observed. The plasma and adrenal cortisol concentrations of the mother were much higher than those observed in the foetus and increased significantly before parturition. In the mother as in the foetus, cortisone and corticosterone represent only a small percentage of corticosteroids compared with cortisol. These results indicate that the autonomous capacity of foetal adrenals, inhibited by maternal secretions before term, appears suddenly at birth.", "PMID": 965881}
{"id": "pubmed23n0032_6485", "title": "Sustained effects on synthetic ovarian steroids of rat myometrial contractility.", "content": "Ovariectomized rats were given a single dose of synthetic oestrogen or progestogen, or both combined. On selected days after treatment one rat from each treatment group was killed and myometrical contractility in response to spasmogens was measured isometrically in isolated tissue baths. Contractility persisted at a low level after ovariectomy without steroid replacement (controls) for the 60 days of the experiment. The powerful potentiating action of oestrogen reached a maximum in about 10 days and sustained a high level thereafter. Progestogen treatment did not influence contractility to a significant degree when compared with saline, except that the contractions were sometimes of higher frequency and more irregular in size. There was a less powerful potentiating action on contractility with combined oestrogen and progestogen treatment than with oestrogen alone. It reached a maximum in 4 days and declined more rapidly than with oestrogen-treated preparation but was still vigorous for up to 30 days.", "contents": "Sustained effects on synthetic ovarian steroids of rat myometrial contractility. Ovariectomized rats were given a single dose of synthetic oestrogen or progestogen, or both combined. On selected days after treatment one rat from each treatment group was killed and myometrical contractility in response to spasmogens was measured isometrically in isolated tissue baths. Contractility persisted at a low level after ovariectomy without steroid replacement (controls) for the 60 days of the experiment. The powerful potentiating action of oestrogen reached a maximum in about 10 days and sustained a high level thereafter. Progestogen treatment did not influence contractility to a significant degree when compared with saline, except that the contractions were sometimes of higher frequency and more irregular in size. There was a less powerful potentiating action on contractility with combined oestrogen and progestogen treatment than with oestrogen alone. It reached a maximum in 4 days and declined more rapidly than with oestrogen-treated preparation but was still vigorous for up to 30 days.", "PMID": 965882}
{"id": "pubmed23n0032_6486", "title": "Testosterone concentration in the fluids of seminiferous tubules, the interstitium and the rete testis of the rat.", "content": "Testosterone was measured by radioimmunoassay in interstitial fluid, 'free-flow' seminiferous tubular fluid, obtained by micropuncture, and rete testis fluid from intact adult anaesthetized rats. Under non-stimulated conditions the concentration of testosterone in interstitial fluid was below the limit of detection in two rats and achieved a mean level of 150 +/- 27 (S.E.M.) ng/ml in the remaining 17 determinations. The testosterone concentration of the seminiferous tubular fluid was below the limit of detection in two rats, and had a mean level in the remaining 15 determinations of 91 +/- 14 ng/ml, which is significantly lower (P less than 0-02) than that in interstitial fluid. The mean ratio of seminiferous tubular:interstitial fluid testosterone concentration calculated in 14 rats was 0-94 +/- 0.24. This ratio was less than unity whenever the interstitial fluid testosterone concentration was more than 50 ng/ml, whereas in all animals with interstitial fluid testosterone of 50 ng/ml, or less, the ratio was greater than or equal to one. The mean testosterone concentration of rete testis fluid in 32 samples was 33 +/- 3 ng/ml. After HCG stimulation in 12 rats, testosterone concentration in interstitial fluid increased to a mean value of 660 +/- 83 ng/ml, and in seminiferous tubular fluid to 460 +/- 44 ng/ml; the difference between the two was significant (P less than 0-05). These results are discussed in relation to the presumed dilution of seminiferous tubular fluid in rete testis fluid and the role of androgen-binding proteins in the transport of steroids.", "contents": "Testosterone concentration in the fluids of seminiferous tubules, the interstitium and the rete testis of the rat. Testosterone was measured by radioimmunoassay in interstitial fluid, 'free-flow' seminiferous tubular fluid, obtained by micropuncture, and rete testis fluid from intact adult anaesthetized rats. Under non-stimulated conditions the concentration of testosterone in interstitial fluid was below the limit of detection in two rats and achieved a mean level of 150 +/- 27 (S.E.M.) ng/ml in the remaining 17 determinations. The testosterone concentration of the seminiferous tubular fluid was below the limit of detection in two rats, and had a mean level in the remaining 15 determinations of 91 +/- 14 ng/ml, which is significantly lower (P less than 0-02) than that in interstitial fluid. The mean ratio of seminiferous tubular:interstitial fluid testosterone concentration calculated in 14 rats was 0-94 +/- 0.24. This ratio was less than unity whenever the interstitial fluid testosterone concentration was more than 50 ng/ml, whereas in all animals with interstitial fluid testosterone of 50 ng/ml, or less, the ratio was greater than or equal to one. The mean testosterone concentration of rete testis fluid in 32 samples was 33 +/- 3 ng/ml. After HCG stimulation in 12 rats, testosterone concentration in interstitial fluid increased to a mean value of 660 +/- 83 ng/ml, and in seminiferous tubular fluid to 460 +/- 44 ng/ml; the difference between the two was significant (P less than 0-05). These results are discussed in relation to the presumed dilution of seminiferous tubular fluid in rete testis fluid and the role of androgen-binding proteins in the transport of steroids.", "PMID": 965883}
{"id": "pubmed23n0032_6487", "title": "Stimulation of nucleic acid and protein synthesis in the epididymis and accessory organs of the rat by testosterone.", "content": "The effects of a single dose to testosterone on the content of DNA, RNA and protein and the incorporation of [3H]phenylalanine into protein in the epididymis, vas deferens and ventral prostate of the rat were studied. A single dose of testosterone did not increase the weights of the accessory organs but restored the incorporation of [3H]phenylalanine into proteins in the catput, corpus and cauda epididymides, vas deferens and ventral prostate to the normal level. Within 1 h of hormone administration, significant increases in the content of DNA, RNA and protein were noticed in the cauda epididymidis and ventral prostate. The catput and corpus epididymides and was deferens whoed decreasing order to responsiveness to testosterone. These data are discussed with respect to the relative responsiveness of these organs to reinitiation to their function by administration of a single dose of testosterone. The new protein(s) synthesized in response to hormonal stimulation associated with growth and secretory activity of the cells may be different from the new protein macromolecules synthesized after hormone withdrawal or inhibition of hormone action which are involved in autolytic processes.", "contents": "Stimulation of nucleic acid and protein synthesis in the epididymis and accessory organs of the rat by testosterone. The effects of a single dose to testosterone on the content of DNA, RNA and protein and the incorporation of [3H]phenylalanine into protein in the epididymis, vas deferens and ventral prostate of the rat were studied. A single dose of testosterone did not increase the weights of the accessory organs but restored the incorporation of [3H]phenylalanine into proteins in the catput, corpus and cauda epididymides, vas deferens and ventral prostate to the normal level. Within 1 h of hormone administration, significant increases in the content of DNA, RNA and protein were noticed in the cauda epididymidis and ventral prostate. The catput and corpus epididymides and was deferens whoed decreasing order to responsiveness to testosterone. These data are discussed with respect to the relative responsiveness of these organs to reinitiation to their function by administration of a single dose of testosterone. The new protein(s) synthesized in response to hormonal stimulation associated with growth and secretory activity of the cells may be different from the new protein macromolecules synthesized after hormone withdrawal or inhibition of hormone action which are involved in autolytic processes.", "PMID": 965884}
{"id": "pubmed23n0032_6488", "title": "Branchial sodium exchange in seawater-adapted Tilapia mossambica: effects of prolactin and hypophysectomy.", "content": "The effects of exogenous prolactin and hypophysectomy were tested on Na exchange across the gills of Tilapia mossambica adapted to sea-water. Exogenous prolactin produced a 75% decrease of both total Na influx and Na efflux. Total Na influx was measured simultaneously with the drinking rate. Water ingestion stopped almost completely in prolactin-treated fish. Thus branchial net excretion flux of Na was reduced 75% by prolactin. Hypophysectomy produced a 50% inhibition of Na efflux across the gill.", "contents": "Branchial sodium exchange in seawater-adapted Tilapia mossambica: effects of prolactin and hypophysectomy. The effects of exogenous prolactin and hypophysectomy were tested on Na exchange across the gills of Tilapia mossambica adapted to sea-water. Exogenous prolactin produced a 75% decrease of both total Na influx and Na efflux. Total Na influx was measured simultaneously with the drinking rate. Water ingestion stopped almost completely in prolactin-treated fish. Thus branchial net excretion flux of Na was reduced 75% by prolactin. Hypophysectomy produced a 50% inhibition of Na efflux across the gill.", "PMID": 965885}
{"id": "pubmed23n0032_6489", "title": "Carcino-embryonic antigen activity in urine of patients with bladder carcinoma. Clinical evaluation of carcino-embryonic antigen. II.", "content": "This paper deals with the study of CEA levels in urine from healthy control subjects, of patients with bladder carcinoma or previous bladder carcinoma, and other groups of patients. In 53% of the patients with bladder carcinoma and without urinary infection, urinary CEA was increased. There were no false positive results. It is concluded that urinary CEA is a valuable aid in the detection of malignant tumour growth in the bladder. The results are compared with several tumour parameters, e.g. tumour size, stage of disease and histological parameters. Evidence is presented that CEA in infected urine is not produced by bacteria and that, like soluble vaginal excretory products, this substance has antigenic groupings in common with CEA from bladder tumours.", "contents": "Carcino-embryonic antigen activity in urine of patients with bladder carcinoma. Clinical evaluation of carcino-embryonic antigen. II. This paper deals with the study of CEA levels in urine from healthy control subjects, of patients with bladder carcinoma or previous bladder carcinoma, and other groups of patients. In 53% of the patients with bladder carcinoma and without urinary infection, urinary CEA was increased. There were no false positive results. It is concluded that urinary CEA is a valuable aid in the detection of malignant tumour growth in the bladder. The results are compared with several tumour parameters, e.g. tumour size, stage of disease and histological parameters. Evidence is presented that CEA in infected urine is not produced by bacteria and that, like soluble vaginal excretory products, this substance has antigenic groupings in common with CEA from bladder tumours.", "PMID": 965892}
{"id": "pubmed23n0032_6490", "title": "Clinical significance of urinary carcino-embryonic antigen estimations during the follow-up of patients with bladder carcinoma or previous bladder carcinoma. Clinical evaluation of carcino-embryonic antigen, III.", "content": "Urinary carcino-embryonic antigen (CEA) was measured in a follow-up study of 101 bladder carcinoma patients. Urinary CEA estimation during follow-up appears to be of clinical value. Negative results can be interpreted only in relation to previously increased values. Positive results are of value in retrospect when combined with negative data, e.g. in evaluation of suspicious bladder changes and as an early indication of renewed tumour growth. Increased CEA levels in urines from patients with urinary infection rapidly decline once the infection is cured.", "contents": "Clinical significance of urinary carcino-embryonic antigen estimations during the follow-up of patients with bladder carcinoma or previous bladder carcinoma. Clinical evaluation of carcino-embryonic antigen, III. Urinary carcino-embryonic antigen (CEA) was measured in a follow-up study of 101 bladder carcinoma patients. Urinary CEA estimation during follow-up appears to be of clinical value. Negative results can be interpreted only in relation to previously increased values. Positive results are of value in retrospect when combined with negative data, e.g. in evaluation of suspicious bladder changes and as an early indication of renewed tumour growth. Increased CEA levels in urines from patients with urinary infection rapidly decline once the infection is cured.", "PMID": 965893}
{"id": "pubmed23n0032_6491", "title": "Turbidimetric determination of haptoglobin.", "content": "A specific, accurate and simple determination of haptoglobin is described with an antiserum against the antigenic determinants of the three haptoglobin phenotypes 1-1, 2-1 and 2-2. Antihaptoglobin is the determining factor in the formation of an antigen-antibody complex with maximal light absorption properties. Haptoglobin concentration is expressed as hemoglobin binding capacity. For the three haptoglobin phenotypes we found similar correlation between the turbidimetric method and the direct measurement of hemoglobin binding capacity by agar-electrophoresis.", "contents": "Turbidimetric determination of haptoglobin. A specific, accurate and simple determination of haptoglobin is described with an antiserum against the antigenic determinants of the three haptoglobin phenotypes 1-1, 2-1 and 2-2. Antihaptoglobin is the determining factor in the formation of an antigen-antibody complex with maximal light absorption properties. Haptoglobin concentration is expressed as hemoglobin binding capacity. For the three haptoglobin phenotypes we found similar correlation between the turbidimetric method and the direct measurement of hemoglobin binding capacity by agar-electrophoresis.", "PMID": 965894}
{"id": "pubmed23n0032_6492", "title": "A new enzymatic determination of cholesterol. The use of aldehyde dehydrogenase to measure H2O2 producing reactions, II.", "content": "Cholesterol oxidase, coupled with the catalase and aldehyde dehydrogenase, is proposed for the determination of cholesterol. The main advantages of this procedure over comparable methods employing cholesterol oxidase are its short reaction time and the use of NADP which permits the direct calculation of the cholesterol concentration from the absorbance value.", "contents": "A new enzymatic determination of cholesterol. The use of aldehyde dehydrogenase to measure H2O2 producing reactions, II. Cholesterol oxidase, coupled with the catalase and aldehyde dehydrogenase, is proposed for the determination of cholesterol. The main advantages of this procedure over comparable methods employing cholesterol oxidase are its short reaction time and the use of NADP which permits the direct calculation of the cholesterol concentration from the absorbance value.", "PMID": 965895}
{"id": "pubmed23n0032_6493", "title": "[Experience with the glucose-dehydrogenase-UV-method for the determination of blood glucose (author's transl)].", "content": "A method for glucose determination using glucose-dehydrogenase is described. Our experience with this method is based on 70 000 determinations of blood glucose within six months. Linearity over a wide range, accuracy and specificity highly recommend this method. No interference was noticed by substances physiologically occuring in blood. The glucose-dehydrogenase-method correlated well with the hexokinase glucose-6-phosphatedehydrogenase method for the determination of blood glucose.", "contents": "[Experience with the glucose-dehydrogenase-UV-method for the determination of blood glucose (author's transl)]. A method for glucose determination using glucose-dehydrogenase is described. Our experience with this method is based on 70 000 determinations of blood glucose within six months. Linearity over a wide range, accuracy and specificity highly recommend this method. No interference was noticed by substances physiologically occuring in blood. The glucose-dehydrogenase-method correlated well with the hexokinase glucose-6-phosphatedehydrogenase method for the determination of blood glucose.", "PMID": 965896}
{"id": "pubmed23n0032_6494", "title": "[Chromatographic investigation of the substrate properties of 8-bromo-ATP in the nucleoside diphosphate kinase reaction (author's transl)].", "content": "8-bromo-ATP is a substrate of nucleoside diphosphate kinase. Its reactivity is similar to that of ATP, and the reaction appears to be unaffected by the different conformations of these two substrates. The nucleoside diphosphate kinase reaction with 8-bromo-ATP cannot be monitored photometrically (optical test) using an NADH/NAD+-dependent enzyme system, because the product 8-bromo-ADP is not a substrate for pyruvate kinase. Measurements of the nucleoside diphosphate kinase reaction with 8-bromo-ATP therefore, were carried out by chromatographic analysis of the nucleoside-triphosphate/diphosphate ratio.", "contents": "[Chromatographic investigation of the substrate properties of 8-bromo-ATP in the nucleoside diphosphate kinase reaction (author's transl)]. 8-bromo-ATP is a substrate of nucleoside diphosphate kinase. Its reactivity is similar to that of ATP, and the reaction appears to be unaffected by the different conformations of these two substrates. The nucleoside diphosphate kinase reaction with 8-bromo-ATP cannot be monitored photometrically (optical test) using an NADH/NAD+-dependent enzyme system, because the product 8-bromo-ADP is not a substrate for pyruvate kinase. Measurements of the nucleoside diphosphate kinase reaction with 8-bromo-ATP therefore, were carried out by chromatographic analysis of the nucleoside-triphosphate/diphosphate ratio.", "PMID": 965897}
{"id": "pubmed23n0032_6495", "title": "A spectrophotometric method for the determination of monoglyceride hydrolase.", "content": "A monoglyceride hydrolase assay is introduced in which the activity of the enzyme is determined by the use of coupled enzymatic reactions. Measurements are easy to perform on a conventional photometer; they are specific, precise and show good reproducibility.", "contents": "A spectrophotometric method for the determination of monoglyceride hydrolase. A monoglyceride hydrolase assay is introduced in which the activity of the enzyme is determined by the use of coupled enzymatic reactions. Measurements are easy to perform on a conventional photometer; they are specific, precise and show good reproducibility.", "PMID": 965898}
{"id": "pubmed23n0032_6496", "title": "Gas chromatographic estimation of homovanillic acid in serum of normals and psychotic patients.", "content": "Homovanillic acid is extracted from 0.5 ml serum with ethyl acetate at acidic pH, and its pentafluoropropionic anhydride and hexafluoroisopropanol derivative injected into a 3% SE-30 column at 140 degrees C, fitted with an electron capture detector. In a group of normal volunteers (n = 42) a mean value of 62+/-45 mug/l was found. The distribution of the serum concentrations was found to be bimodal. Using the same procedure for the homovanillic acid estimation in cerebrospinal fluid (CSF), mean values of 73.0+/-41 mug/l serum and 68+/-36 mug/l CSF were found for a group of 22 untreated patients with paranoid-hallucinatory syndrome. After treatment with neuroleptics, for 1 week to 1 month, the homovanillic acid concentration increased significantly only in the CSF. By the procedure described, other acidic metabolites of biogenic amines are extracted together with homovanillic acid and can also be estimated in the same sample.", "contents": "Gas chromatographic estimation of homovanillic acid in serum of normals and psychotic patients. Homovanillic acid is extracted from 0.5 ml serum with ethyl acetate at acidic pH, and its pentafluoropropionic anhydride and hexafluoroisopropanol derivative injected into a 3% SE-30 column at 140 degrees C, fitted with an electron capture detector. In a group of normal volunteers (n = 42) a mean value of 62+/-45 mug/l was found. The distribution of the serum concentrations was found to be bimodal. Using the same procedure for the homovanillic acid estimation in cerebrospinal fluid (CSF), mean values of 73.0+/-41 mug/l serum and 68+/-36 mug/l CSF were found for a group of 22 untreated patients with paranoid-hallucinatory syndrome. After treatment with neuroleptics, for 1 week to 1 month, the homovanillic acid concentration increased significantly only in the CSF. By the procedure described, other acidic metabolites of biogenic amines are extracted together with homovanillic acid and can also be estimated in the same sample.", "PMID": 965899}
{"id": "pubmed23n0032_6497", "title": "[Bivariate evaluation of laboratory findings: immunoglobulin G and albumin in cerebrospinal fluid (author's transl)].", "content": "Using radial immunodiffusion, albumin and immunoglobulin G were determined in non-preconcentrated cerebrospinal fluid from 127 controls and from 239 patients. In controls the concentrations of albumin and immunoglobulin G followed normal distribution. The two variables were correlated linearly (r = 0.60). The elliptic bivariate normal range was calculated, and was found to contain 95% of the paired values. As a clinical limit, this range discriminated more effectively between normal and altered pairs than the two one-dimensional normal ranges X+/-2 s, thus improving the evaluation of laboratory findings in the single case. Likewise in clinically defined groups of patients, bivariate evaluation of results provided additional evidence. In many distinct clinical syndromes, e.g. bacterial encephalomeningitis, polyneuropathy, amyotrophic lateral sclerosis, albumin and immunoglobulin G concentrations exhibited an especially close correlation, probably resulting from damage to the blood-cerebrospinal fluid barrier. However, no correlation of these two variables was detected in acute encephalomeningitis due to virus infection, and in multiple sclerosis: in these groups, immunoglobulin G concentrations were elevated independently of albumin. Since evidence is lacking as to the pathogenesis of multiple sclerosis, it seems noteworthy that the same phenomenon was observed in a well-defined group of viral infections.", "contents": "[Bivariate evaluation of laboratory findings: immunoglobulin G and albumin in cerebrospinal fluid (author's transl)]. Using radial immunodiffusion, albumin and immunoglobulin G were determined in non-preconcentrated cerebrospinal fluid from 127 controls and from 239 patients. In controls the concentrations of albumin and immunoglobulin G followed normal distribution. The two variables were correlated linearly (r = 0.60). The elliptic bivariate normal range was calculated, and was found to contain 95% of the paired values. As a clinical limit, this range discriminated more effectively between normal and altered pairs than the two one-dimensional normal ranges X+/-2 s, thus improving the evaluation of laboratory findings in the single case. Likewise in clinically defined groups of patients, bivariate evaluation of results provided additional evidence. In many distinct clinical syndromes, e.g. bacterial encephalomeningitis, polyneuropathy, amyotrophic lateral sclerosis, albumin and immunoglobulin G concentrations exhibited an especially close correlation, probably resulting from damage to the blood-cerebrospinal fluid barrier. However, no correlation of these two variables was detected in acute encephalomeningitis due to virus infection, and in multiple sclerosis: in these groups, immunoglobulin G concentrations were elevated independently of albumin. Since evidence is lacking as to the pathogenesis of multiple sclerosis, it seems noteworthy that the same phenomenon was observed in a well-defined group of viral infections.", "PMID": 965900}
{"id": "pubmed23n0032_6498", "title": "[Determination of the activity of peptidases and amino acid aryl amidases (author's transl)].", "content": "A modification of the Bratton-Marshall reaction for the quantitative determination of enzymically released p-nitroaniline is described. It is very sensitive, easily performed, shows good reproducibility, and it is suitable for routine use in clinical chemistry.", "contents": "[Determination of the activity of peptidases and amino acid aryl amidases (author's transl)]. A modification of the Bratton-Marshall reaction for the quantitative determination of enzymically released p-nitroaniline is described. It is very sensitive, easily performed, shows good reproducibility, and it is suitable for routine use in clinical chemistry.", "PMID": 965901}
{"id": "pubmed23n0032_6499", "title": "Critical evaluation of measurement of platelet monoamine oxidase in man.", "content": "Some biochemical characteristics such as substrate specificity, substrate affinity and inhibitor sensitivity of monoamine oxidase of human blood platelets were investigated. Tyramine, tryptamine and beta-phenylethylamine were used as substrates. The apparent Michaelis constants, maximal velocity rates and I50 for the inhibitor tranylcypromine were determined. The data were analyzed according to Lineweaver-Burk and Dixon. The influence of amitriptyline, a prototype of tricyclic antidepressants, on the selected variables (Km, V, I50), was studied. The parameters investigated showed remarkably low interindividual differences when healthy volunteers were tested. The inhibitor activity of amitriptyline towards platelet monoamine oxidase depends on the substrate used. Amitriptyline concentrations which showed a pronounced effect on the enzyme characteristics are significantly higher than plasma levels of the drug found under therapeutic conditions.", "contents": "Critical evaluation of measurement of platelet monoamine oxidase in man. Some biochemical characteristics such as substrate specificity, substrate affinity and inhibitor sensitivity of monoamine oxidase of human blood platelets were investigated. Tyramine, tryptamine and beta-phenylethylamine were used as substrates. The apparent Michaelis constants, maximal velocity rates and I50 for the inhibitor tranylcypromine were determined. The data were analyzed according to Lineweaver-Burk and Dixon. The influence of amitriptyline, a prototype of tricyclic antidepressants, on the selected variables (Km, V, I50), was studied. The parameters investigated showed remarkably low interindividual differences when healthy volunteers were tested. The inhibitor activity of amitriptyline towards platelet monoamine oxidase depends on the substrate used. Amitriptyline concentrations which showed a pronounced effect on the enzyme characteristics are significantly higher than plasma levels of the drug found under therapeutic conditions.", "PMID": 965902}
{"id": "pubmed23n0032_6500", "title": "On the self-regulation of the occipital alpha rhythm: control strategies, states of consciousness, and the role of physiological feedback.", "content": "In an experiment investigating the self-control of occipital alpha strength, a factorial design was employed with two levels of feedback (available or non-available), three levels of instruction (Cognitive, Oculomotor, and none), and two levels of lighting (on and off), except that the no-feedback-no-instruction group was not run. The Cognitive instructions were based on the strategies reported in the literature: To generate alpha, persons were instructed to relax, \"let go\", try to feel pleasant and serene, and allow themselves to review pleasant personal experiences; to suppress alpha, persons were instructed to concentrate, try hard, exert themselves mentally, and try to fell anxious or frustrated. The Oculomotor instructions to generate alpha were to visually blur and not focus, and to suppress alpha were to \"look\" and focus. The results led to the following conclusions: (a) Feedback-augmented enhancement and suppression of occipital alpha strength is always mediated by learned control of oculomotor processes, although sometimes persons are not aware that they are employing this strategy, especially in the case of alpha enhancement; alpha feedback trainees learn to \"not look\" in order to enhance alpha strength. (b) Whenever Cognitive strategies are successful in producing alpha strength changes, this is due to the fact that they can be efficient mediators of changes in oculomotor processes. (c) Feedback together with simple Oculomotor instructions leads to more successful alpha control than either alone. (d) High or enhanced levels of occipital alpha strength are not invariably accompanied by the \"alpha experience\", in fact, if a person is not led to expect it, the alpha experience will usually not occur during occipital alpha enhancement feedback. (e) States of nonsensory awareness, such as an absorption in thoughts and feelings, are reliably associated with enhanced occipital alpha strength. This association is what would be expected: Since vision is the dominant sensory modality in humans, it is reasonable that when visual processing is reduced during alpha enhancement feedback that there is also reduced sensory awareness in general and often greater attention to thoughts and feelings. (f) Alpha feedback per se is neither necessary for nor especially facilitative of the achievement of the alpha experience. Thus, if the alpha experience is more likely to occur with alpha feedback than without it, this is due to the special circumstances that make up the feedback situation and not due to alpha feedback per se.", "contents": "On the self-regulation of the occipital alpha rhythm: control strategies, states of consciousness, and the role of physiological feedback. In an experiment investigating the self-control of occipital alpha strength, a factorial design was employed with two levels of feedback (available or non-available), three levels of instruction (Cognitive, Oculomotor, and none), and two levels of lighting (on and off), except that the no-feedback-no-instruction group was not run. The Cognitive instructions were based on the strategies reported in the literature: To generate alpha, persons were instructed to relax, \"let go\", try to feel pleasant and serene, and allow themselves to review pleasant personal experiences; to suppress alpha, persons were instructed to concentrate, try hard, exert themselves mentally, and try to fell anxious or frustrated. The Oculomotor instructions to generate alpha were to visually blur and not focus, and to suppress alpha were to \"look\" and focus. The results led to the following conclusions: (a) Feedback-augmented enhancement and suppression of occipital alpha strength is always mediated by learned control of oculomotor processes, although sometimes persons are not aware that they are employing this strategy, especially in the case of alpha enhancement; alpha feedback trainees learn to \"not look\" in order to enhance alpha strength. (b) Whenever Cognitive strategies are successful in producing alpha strength changes, this is due to the fact that they can be efficient mediators of changes in oculomotor processes. (c) Feedback together with simple Oculomotor instructions leads to more successful alpha control than either alone. (d) High or enhanced levels of occipital alpha strength are not invariably accompanied by the \"alpha experience\", in fact, if a person is not led to expect it, the alpha experience will usually not occur during occipital alpha enhancement feedback. (e) States of nonsensory awareness, such as an absorption in thoughts and feelings, are reliably associated with enhanced occipital alpha strength. This association is what would be expected: Since vision is the dominant sensory modality in humans, it is reasonable that when visual processing is reduced during alpha enhancement feedback that there is also reduced sensory awareness in general and often greater attention to thoughts and feelings. (f) Alpha feedback per se is neither necessary for nor especially facilitative of the achievement of the alpha experience. Thus, if the alpha experience is more likely to occur with alpha feedback than without it, this is due to the special circumstances that make up the feedback situation and not due to alpha feedback per se.", "PMID": 965905}
{"id": "pubmed23n0032_6501", "title": "An analytical model for ovarian follicle dynamics.", "content": "Differential counts of follicles of different sizes in the ovaries of intact and hypophysectomized strain A mice of various ages have been analysed statistically. The influence of age on the number of follicles in each size group is described. The number of small follicles in the ovarian pool declined exponentially, whereas numbers of follicles in other groups displayed two peaks, one during immaturity and the other in adult life. These appeared sequentially in follicles in successive groups. The proportion of follicles growing and dying was estimated and age dependent fluctuations in the time spent by follicles in each group were demonstrated. Further calculations then determined the mean inflow and outflow of follicles to and from the various groups throughout the lifespan. Hypophysectomy during post-puberty decreased only slightly the utilization of small follicles from the pool and did not prevent their subsequent development to the two-layered stage. Many follicles then died at this stage of development, indicating that the initial main site of gonadotrophic action may be on medium sized follicles with two layers of granulosa cells.", "contents": "An analytical model for ovarian follicle dynamics. Differential counts of follicles of different sizes in the ovaries of intact and hypophysectomized strain A mice of various ages have been analysed statistically. The influence of age on the number of follicles in each size group is described. The number of small follicles in the ovarian pool declined exponentially, whereas numbers of follicles in other groups displayed two peaks, one during immaturity and the other in adult life. These appeared sequentially in follicles in successive groups. The proportion of follicles growing and dying was estimated and age dependent fluctuations in the time spent by follicles in each group were demonstrated. Further calculations then determined the mean inflow and outflow of follicles to and from the various groups throughout the lifespan. Hypophysectomy during post-puberty decreased only slightly the utilization of small follicles from the pool and did not prevent their subsequent development to the two-layered stage. Many follicles then died at this stage of development, indicating that the initial main site of gonadotrophic action may be on medium sized follicles with two layers of granulosa cells.", "PMID": 965906}
{"id": "pubmed23n0032_6502", "title": "Effects of epididymal occlusion on sperm maturation in the hamster.", "content": "Hamster epididymal spermatozoa were isolated in the caput, corpus and cauda regions for one, two and three days. Sperm suspensions from these regions were assessed for morphology, motility, viability; and for fertility by A.I. The ability to capacitate was tested by checking for acrosome reactions when the sperm were cultured under defined capacitating conditions at 37 degrees C in vitro. Normal, mature cauda sperm were highly fertile (overall 77% of ova fertilized) and showed 50-80% incidence of acrosome reactions after six hours in culture. Isolation for up to three days had no effect. Immature sperm from the caput and corpus were poorly motile, infertile, and did not manifest an acrosome reaction. Isolation for one and two days produced improvements in motility, and distal migration of the cytoplasmic droplet skin to normal maturation, however the sperm remained infertile and did not capacitate in vitro. Survival after three days isolation was poor. Thus the development of fertilizing ability in hamster epididymal spermatozoa is closely related to the ability to manifest an acrosome reaction in vitro; however it is only poorly correlated with motility and morphology. Completion of sperm maturation in this species appears to require the environment of the cauda epididymidis.", "contents": "Effects of epididymal occlusion on sperm maturation in the hamster. Hamster epididymal spermatozoa were isolated in the caput, corpus and cauda regions for one, two and three days. Sperm suspensions from these regions were assessed for morphology, motility, viability; and for fertility by A.I. The ability to capacitate was tested by checking for acrosome reactions when the sperm were cultured under defined capacitating conditions at 37 degrees C in vitro. Normal, mature cauda sperm were highly fertile (overall 77% of ova fertilized) and showed 50-80% incidence of acrosome reactions after six hours in culture. Isolation for up to three days had no effect. Immature sperm from the caput and corpus were poorly motile, infertile, and did not manifest an acrosome reaction. Isolation for one and two days produced improvements in motility, and distal migration of the cytoplasmic droplet skin to normal maturation, however the sperm remained infertile and did not capacitate in vitro. Survival after three days isolation was poor. Thus the development of fertilizing ability in hamster epididymal spermatozoa is closely related to the ability to manifest an acrosome reaction in vitro; however it is only poorly correlated with motility and morphology. Completion of sperm maturation in this species appears to require the environment of the cauda epididymidis.", "PMID": 965907}
{"id": "pubmed23n0032_6503", "title": "Changes in the shape of the developing vertebrate nervous system analyzed experimentally, mathematically and by computer simulation.", "content": "Two forces are necessary and sufficient to produce the transformation of the newt neural plate from a hemispheric sheet of cells one cell thick to a keyhole shape. These forces are: (1) a regionally programmed shrinkage of the surface of the neural plate (accomplished by contraction of the apical surfaces of the neural plate cells and elongation of these cells perpendicular to the plate); and (2) displacement of the whole sheet caused by elongation of either the notochord or the overlying neural plate cells in the antero-posterior direction. A computer simulation and mathematical analysis (\"morphodynamics\"), together with experiments and observations on embryos, were used to deduce the morphogenesis of the neural plate from these forces.", "contents": "Changes in the shape of the developing vertebrate nervous system analyzed experimentally, mathematically and by computer simulation. Two forces are necessary and sufficient to produce the transformation of the newt neural plate from a hemispheric sheet of cells one cell thick to a keyhole shape. These forces are: (1) a regionally programmed shrinkage of the surface of the neural plate (accomplished by contraction of the apical surfaces of the neural plate cells and elongation of these cells perpendicular to the plate); and (2) displacement of the whole sheet caused by elongation of either the notochord or the overlying neural plate cells in the antero-posterior direction. A computer simulation and mathematical analysis (\"morphodynamics\"), together with experiments and observations on embryos, were used to deduce the morphogenesis of the neural plate from these forces.", "PMID": 965908}
{"id": "pubmed23n0032_6504", "title": "Response of the ovary in the catfish, Heteropneustes fossilis (Bloch), to various combinations of photoperiod and temperature.", "content": "Responses of the ovary of the catfish, Heteropneustes fossilis, to various regimes of photoperiod and temperature, were studied during the different periods of the annual ovarian cycle. During the preparatory and post-spawning periods moderate temperatures of 25 degrees C and above, regardless of photoperiod, were more favorable for the formation of yolky (stage III) oocytes than coller temperatures (below 23 degrees C). Even under conditions of continuous dark or light, ovarian development occurred as a function of warm temperatures. Gravid ovaries were maintained beyond the spawning period only with a temperature of 30 degrees C, photoperiod notwithstanding. Nevertheless, moderate or cool temperatures did not prevent the ovarian regression that occurred by the first week of October in the post-spawning period. Regulation of ovarian activity at least in part by an endogenous circannual rhythm appears possible.", "contents": "Response of the ovary in the catfish, Heteropneustes fossilis (Bloch), to various combinations of photoperiod and temperature. Responses of the ovary of the catfish, Heteropneustes fossilis, to various regimes of photoperiod and temperature, were studied during the different periods of the annual ovarian cycle. During the preparatory and post-spawning periods moderate temperatures of 25 degrees C and above, regardless of photoperiod, were more favorable for the formation of yolky (stage III) oocytes than coller temperatures (below 23 degrees C). Even under conditions of continuous dark or light, ovarian development occurred as a function of warm temperatures. Gravid ovaries were maintained beyond the spawning period only with a temperature of 30 degrees C, photoperiod notwithstanding. Nevertheless, moderate or cool temperatures did not prevent the ovarian regression that occurred by the first week of October in the post-spawning period. Regulation of ovarian activity at least in part by an endogenous circannual rhythm appears possible.", "PMID": 965909}
{"id": "pubmed23n0032_6505", "title": "Protein synthesis in lethal nucleocytoplasmic hybrids between the species Pleurodeles waltlii Michahelles and Ambystoma mexicanum Shaw (urodele amphibians) obtained by nuclear grafting.", "content": "Using polyacrylamide gel electrophoresis, proteins stemming from the nucleus of the donor species in nucleocytoplasmic hybrids were determined. The interspecific lethal nucleocytoplasmic hybrids of Pleurodeles waltlii and Ambystoma mexicanum were studied. The presence of these proteins at the blastula or early gastrula stages attests to the early nuclear control of their synthesis.", "contents": "Protein synthesis in lethal nucleocytoplasmic hybrids between the species Pleurodeles waltlii Michahelles and Ambystoma mexicanum Shaw (urodele amphibians) obtained by nuclear grafting. Using polyacrylamide gel electrophoresis, proteins stemming from the nucleus of the donor species in nucleocytoplasmic hybrids were determined. The interspecific lethal nucleocytoplasmic hybrids of Pleurodeles waltlii and Ambystoma mexicanum were studied. The presence of these proteins at the blastula or early gastrula stages attests to the early nuclear control of their synthesis.", "PMID": 965910}
{"id": "pubmed23n0032_6506", "title": "A role for esterases in the fertilization process.", "content": "The corona penetrating enzyme of sperm is shown to be esterolytic in nature. This is the first known function of the several sperm esterases and indicates the cementing substance between cells of the corona radiata contains an essential ester linkage.", "contents": "A role for esterases in the fertilization process. The corona penetrating enzyme of sperm is shown to be esterolytic in nature. This is the first known function of the several sperm esterases and indicates the cementing substance between cells of the corona radiata contains an essential ester linkage.", "PMID": 965911}
{"id": "pubmed23n0032_6507", "title": "Photoperiodic control of antler cycles in deer. III. Decreasing versus increasing day lengths.", "content": "Deer were exposed for three years to photoperiods which increased or decreased two hours every four months, starting at 4L/20D or 20L/4D, respectively. Under both sets of conditions, antlers were repeatedly shed and replaced, usually in synchrony with every other time the day lengths were changed. On decreasing days, antler cycles were omitted as the photoperiod passed the equinox (12L/12D). On increasing days, the equinoctial photoperiod induced prolonged episodes of antler growth. The tendency for the antler replacement cycle to lock onto alternate changes in artificial photoperiods is consistent with the seasonal growth of antlers every other time the day lengths change in the natural environment. It is suggested that antler replacement is triggered neither by shortening nor lengthening days, but by the alternation of such changes irrespective of the direction of the shift in the photoperiod.", "contents": "Photoperiodic control of antler cycles in deer. III. Decreasing versus increasing day lengths. Deer were exposed for three years to photoperiods which increased or decreased two hours every four months, starting at 4L/20D or 20L/4D, respectively. Under both sets of conditions, antlers were repeatedly shed and replaced, usually in synchrony with every other time the day lengths were changed. On decreasing days, antler cycles were omitted as the photoperiod passed the equinox (12L/12D). On increasing days, the equinoctial photoperiod induced prolonged episodes of antler growth. The tendency for the antler replacement cycle to lock onto alternate changes in artificial photoperiods is consistent with the seasonal growth of antlers every other time the day lengths change in the natural environment. It is suggested that antler replacement is triggered neither by shortening nor lengthening days, but by the alternation of such changes irrespective of the direction of the shift in the photoperiod.", "PMID": 965912}
{"id": "pubmed23n0032_6508", "title": "The regeneration of brachial nerves of contralateral origin into benervated fused newt forelimbs.", "content": "Previous studies involving the end-to-end fusion of the forelimbs of the adult newt have demonstrated that new limbs can regenerate from the transected ends of proximo-distally reversed limb segments. The limb regeneration could only have been initiated by nerve fibers of contralateral origin. The purpose of the present study is to describe histologically the manner in which nerve fibers of contralateral origin regenerate through the junction of fused limbs into the opposite limb. The first sign of nerve regeneration into the opposite limb was observed at eight days post fusion. The nerves crossed over into the opposite, originally denervated limb in a highly dispersed manner. These nerve fibers eventually aggregated, however, either under the skin or within persisting nerve trunks. By 19 days post fusion the nerve fibers had reached the elbow region of the originally denervated limb and by 25 days they were seen at the most proximal extent of the limb. The diameters of the axons seemed smaller than the diameters of regenerating axons observed in non-fused newt forelimbs.", "contents": "The regeneration of brachial nerves of contralateral origin into benervated fused newt forelimbs. Previous studies involving the end-to-end fusion of the forelimbs of the adult newt have demonstrated that new limbs can regenerate from the transected ends of proximo-distally reversed limb segments. The limb regeneration could only have been initiated by nerve fibers of contralateral origin. The purpose of the present study is to describe histologically the manner in which nerve fibers of contralateral origin regenerate through the junction of fused limbs into the opposite limb. The first sign of nerve regeneration into the opposite limb was observed at eight days post fusion. The nerves crossed over into the opposite, originally denervated limb in a highly dispersed manner. These nerve fibers eventually aggregated, however, either under the skin or within persisting nerve trunks. By 19 days post fusion the nerve fibers had reached the elbow region of the originally denervated limb and by 25 days they were seen at the most proximal extent of the limb. The diameters of the axons seemed smaller than the diameters of regenerating axons observed in non-fused newt forelimbs.", "PMID": 965913}
{"id": "pubmed23n0032_6509", "title": "Anesthesia as an effective agent against the production of congenital anomalies in mouse fetuses exposed to electromagnetic radiation.", "content": "A total of 130 female mice pregnant at 8 days were exposed singly to a 7.3 watt microwave waveguide at 2,450 MHz wave length while being monitored for total obsorbed dose, the exposure being four minutes. Among the 1,328 offspirng examined (327 of which were controls) those not anesthetized but irradiated showed greater fetal damage than those that were anesthetized and placed in one of the three positions with respect to the radiation beam. Immobilization with anesthesia and restraint in the broadside position provided the greatest protection to the fetus against any teratologic effects. Anesthesia reduces the body temperature to a degree equivalent to the rise in temperature expected from the radiation conditions used, hence the protection against the primary thermal effect of microwave irradiation.", "contents": "Anesthesia as an effective agent against the production of congenital anomalies in mouse fetuses exposed to electromagnetic radiation. A total of 130 female mice pregnant at 8 days were exposed singly to a 7.3 watt microwave waveguide at 2,450 MHz wave length while being monitored for total obsorbed dose, the exposure being four minutes. Among the 1,328 offspirng examined (327 of which were controls) those not anesthetized but irradiated showed greater fetal damage than those that were anesthetized and placed in one of the three positions with respect to the radiation beam. Immobilization with anesthesia and restraint in the broadside position provided the greatest protection to the fetus against any teratologic effects. Anesthesia reduces the body temperature to a degree equivalent to the rise in temperature expected from the radiation conditions used, hence the protection against the primary thermal effect of microwave irradiation.", "PMID": 965914}
{"id": "pubmed23n0032_6510", "title": "Effects of various low temperatures, cryoprotective agents and cooling rates on the survival, fertilizability and development of frozen-thawed mouse eggs.", "content": "Frozen mouse eggs were examined to determine the effects of low temperatures, concentration of cryoprotective agents and cooling rates on their survival, fertilizability in vitro and subsequent development. Dimethyl sulfoxide administered at 1.5 M concentration was found to be the most effective cryoprotective agent. Upon thawing, 51% and 56% of the eggs appeared to be normal after having been cooled at 0.33 degrees C/min to -30 degrees C and -50 degrees C, but only 18% of the eggs appeared to be normal after having been cooled at the same rate to -75 degrees C. When eggs were cooled at 0.33 degrees C/min to -45 degrees C and the cooling rate increased to 1 degree C/min from -45 degrees C to -75 degrees C, 44% and 72% appeared normal upon thawing. Of the normal eggs fertilized in vitro from C3H mice, 65% cleaved to the 2-cell stage and 24% of the 2-cell eggs developed into blastocysts. Following the transfer of 17 blastocysts into three recipient mice, one mouse delivered three normal young.", "contents": "Effects of various low temperatures, cryoprotective agents and cooling rates on the survival, fertilizability and development of frozen-thawed mouse eggs. Frozen mouse eggs were examined to determine the effects of low temperatures, concentration of cryoprotective agents and cooling rates on their survival, fertilizability in vitro and subsequent development. Dimethyl sulfoxide administered at 1.5 M concentration was found to be the most effective cryoprotective agent. Upon thawing, 51% and 56% of the eggs appeared to be normal after having been cooled at 0.33 degrees C/min to -30 degrees C and -50 degrees C, but only 18% of the eggs appeared to be normal after having been cooled at the same rate to -75 degrees C. When eggs were cooled at 0.33 degrees C/min to -45 degrees C and the cooling rate increased to 1 degree C/min from -45 degrees C to -75 degrees C, 44% and 72% appeared normal upon thawing. Of the normal eggs fertilized in vitro from C3H mice, 65% cleaved to the 2-cell stage and 24% of the 2-cell eggs developed into blastocysts. Following the transfer of 17 blastocysts into three recipient mice, one mouse delivered three normal young.", "PMID": 965915}
{"id": "pubmed23n0032_6511", "title": "Alterations in the developmental properties of stroma during the development of the urogenital ridge into ductus deferens and uterus in embryonic and neonatal mice.", "content": "The ability of mesenchyme from the urogenital ridge to participate in the development of male and female urogenital organs was studied by preparing homo- and heterotypic recombinants of epithelium and stroma from ductus deferens and uterus of neonatal mice. The recombinants as well as intact 14-day old embryonic urogenital ridges (URG) were grown for two to eight weeks in adult male and female hosts. The development of ductus deferens and seminal vesicle from female UGR's grafted into male hosts and uterus from male UGR's grafted into female hosts demonstrate that the mesenchyme of embryonic UGR's is capable of participating in the development of either male or female urogenital structures. During normal development of the female urogenital tract, the bisexual morphogenetic potentiality of UGR mesenchyme persists postnatally as demonstrated by the ability of uterine stroma to support normal differentiation of epithelium of ductus deferens in male hosts. Conversely, during the normal development of the male urogenital tract, the morphogenetic potentiality of mesenchyme of the urogenital ridge is restricted as stroma from the ductus deferens appears incapable of participating in uterine morphogenesis in male or female hosts. These differences in the developmental properties of UGR stroma may be attributed to differences in hormonal conditions in male and female fetuses.", "contents": "Alterations in the developmental properties of stroma during the development of the urogenital ridge into ductus deferens and uterus in embryonic and neonatal mice. The ability of mesenchyme from the urogenital ridge to participate in the development of male and female urogenital organs was studied by preparing homo- and heterotypic recombinants of epithelium and stroma from ductus deferens and uterus of neonatal mice. The recombinants as well as intact 14-day old embryonic urogenital ridges (URG) were grown for two to eight weeks in adult male and female hosts. The development of ductus deferens and seminal vesicle from female UGR's grafted into male hosts and uterus from male UGR's grafted into female hosts demonstrate that the mesenchyme of embryonic UGR's is capable of participating in the development of either male or female urogenital structures. During normal development of the female urogenital tract, the bisexual morphogenetic potentiality of UGR mesenchyme persists postnatally as demonstrated by the ability of uterine stroma to support normal differentiation of epithelium of ductus deferens in male hosts. Conversely, during the normal development of the male urogenital tract, the morphogenetic potentiality of mesenchyme of the urogenital ridge is restricted as stroma from the ductus deferens appears incapable of participating in uterine morphogenesis in male or female hosts. These differences in the developmental properties of UGR stroma may be attributed to differences in hormonal conditions in male and female fetuses.", "PMID": 965916}
{"id": "pubmed23n0032_6512", "title": "An ultrastructural analysis of chick embryonic skin development in vitro.", "content": "Behaviour of 6-day chick embryo thigh skin in vitro in two different nutrients was studied electron microscopically. In explants supported with chicken serum containing medium epithelium keratinized faster than and in a similar way to that in ovo, except for the absence of corpuscola cribriformia in pericytes. It did not in explants supported with chick embryo extract containing medium, but underwent a noticeable ultrastructural evolution, mainly of granular reticulum and Golgi complexes. In the two series of cultures mesenchyme presented the same ultrastructural characteristics, especially as far as collagen fibers were concerned. The above data rule out the suggested regulatory role of collagen fibers in epidermal differentiation and support a possible epidermal two-step differentiative process. They are discussed in relation to the general mechanisms implicated in skin evolution.", "contents": "An ultrastructural analysis of chick embryonic skin development in vitro. Behaviour of 6-day chick embryo thigh skin in vitro in two different nutrients was studied electron microscopically. In explants supported with chicken serum containing medium epithelium keratinized faster than and in a similar way to that in ovo, except for the absence of corpuscola cribriformia in pericytes. It did not in explants supported with chick embryo extract containing medium, but underwent a noticeable ultrastructural evolution, mainly of granular reticulum and Golgi complexes. In the two series of cultures mesenchyme presented the same ultrastructural characteristics, especially as far as collagen fibers were concerned. The above data rule out the suggested regulatory role of collagen fibers in epidermal differentiation and support a possible epidermal two-step differentiative process. They are discussed in relation to the general mechanisms implicated in skin evolution.", "PMID": 965917}
{"id": "pubmed23n0032_6513", "title": "Intercellular junctions in the Xenopus embryo prior to gastrulation.", "content": "A freeze-fracture study of Xenopus embryos at the eight-cell stage revealed the presence of tight junctions and small aggregations of 120 A diameter intramembranous particles. These aggregations consisted of up to ten particles and resembled forming gap junctions. Examination of early blastulae showed that fully developed gap junctions were present, apparently occurring very infrequently. These results support the contention that specialized junctions mediate electrical coupling during early amphibian development.", "contents": "Intercellular junctions in the Xenopus embryo prior to gastrulation. A freeze-fracture study of Xenopus embryos at the eight-cell stage revealed the presence of tight junctions and small aggregations of 120 A diameter intramembranous particles. These aggregations consisted of up to ten particles and resembled forming gap junctions. Examination of early blastulae showed that fully developed gap junctions were present, apparently occurring very infrequently. These results support the contention that specialized junctions mediate electrical coupling during early amphibian development.", "PMID": 965918}
{"id": "pubmed23n0032_6514", "title": "The reaquisition of cell adhesiveness following tissue disaggregation by eleven different agents.", "content": "Disaggregation of seven-day embryonic chick neural retina tissue by eleven different agents produces cells which require variable periods of time to become aggregation competent in culture. The time required between cell isolation and the beginning of inter-cellular adhesion in rotation culture is dependent upon the particular isolating agent used and ranges from zero to sixty minutes.", "contents": "The reaquisition of cell adhesiveness following tissue disaggregation by eleven different agents. Disaggregation of seven-day embryonic chick neural retina tissue by eleven different agents produces cells which require variable periods of time to become aggregation competent in culture. The time required between cell isolation and the beginning of inter-cellular adhesion in rotation culture is dependent upon the particular isolating agent used and ranges from zero to sixty minutes.", "PMID": 965919}
{"id": "pubmed23n0032_6515", "title": "Induction of meiosis by injection of heterologous protein kinase and phosphorylase kinase in Xenopus laevis oocytes.", "content": "Meiosis reinitiation has been triggered by injection of beef heart protein kinase or rabbit phosphorylase kinase into Xenopus laevis oocytes. Successful injections are followed by germinal vesicle breakdown, chromosome condensation, formation of a normal meiotic spindle, and appearance of an amplifiable maturation promoting factor. Meiosis reinitiation does not occur when the enzymes are introduced into the oocytes simultaneously with EGTA or after pretreatment with cycloheximide. Antipain, an antiprotease which abolishes the response of oocytes to progesterone, does not suppress the meiosis reinitiation induced by injection of protein kinase or phosphorylase kinase.", "contents": "Induction of meiosis by injection of heterologous protein kinase and phosphorylase kinase in Xenopus laevis oocytes. Meiosis reinitiation has been triggered by injection of beef heart protein kinase or rabbit phosphorylase kinase into Xenopus laevis oocytes. Successful injections are followed by germinal vesicle breakdown, chromosome condensation, formation of a normal meiotic spindle, and appearance of an amplifiable maturation promoting factor. Meiosis reinitiation does not occur when the enzymes are introduced into the oocytes simultaneously with EGTA or after pretreatment with cycloheximide. Antipain, an antiprotease which abolishes the response of oocytes to progesterone, does not suppress the meiosis reinitiation induced by injection of protein kinase or phosphorylase kinase.", "PMID": 965920}
{"id": "pubmed23n0032_6516", "title": "Electrophoretic introduction of calcium ions into the cortex of Xenopus laevis oocytes triggers meiosis neinitiation.", "content": "Meiosis reinitiation has been triggered via electrophoretic driving of Ca++ into the cortex of Xenopus laevis oocytes freed from functional follicle cells by collagenase treatment. Mg++ could not be substituted for Ca++. Ca++ induced maturation resembles progesterone induced maturation in so far both are inhibited by cycloheximide, give rise to amplifiable MPF in the cytoplasm of treated oocytes and allow chromosome condensation and the formation of a normal meiotic spindle.", "contents": "Electrophoretic introduction of calcium ions into the cortex of Xenopus laevis oocytes triggers meiosis neinitiation. Meiosis reinitiation has been triggered via electrophoretic driving of Ca++ into the cortex of Xenopus laevis oocytes freed from functional follicle cells by collagenase treatment. Mg++ could not be substituted for Ca++. Ca++ induced maturation resembles progesterone induced maturation in so far both are inhibited by cycloheximide, give rise to amplifiable MPF in the cytoplasm of treated oocytes and allow chromosome condensation and the formation of a normal meiotic spindle.", "PMID": 965921}
{"id": "pubmed23n0032_6517", "title": "Purification and polypeptide composition of Semliki Forest virus RNA polymerase.", "content": "A purification method for Semliki Forest virus-specified RNA-dependent RNA polymerase from BHK cells is described. The procedure entails (i) the preparation of a crude cell lysate by Dounce homogenization of cells 3-5 h post-infection, (ii) differential centrifugation to give a 15 000 g 'mitochondrial' pellet, (iii) equilibrium centrifugation on discontinuous sucrose gradients (Friedman et al. 1972) to give a membranous band of density 1-16 g/ml, (iv) solubilization with Triton N-101 and velocity centrifugation to give a 25S solubilized polymerase complex and (v) affinity chromatography through an oligo (dT)-cellulose matrix bearing immobilized 42S virus particle RNA. The overall purification was approx. 360-fold with a 5% recovery of activity. Of the various intermediate fractions in the purfication procedure, only the relatively crude post-nuclear supernatant fraction was competent to synthesize the major single-stranded RNAs found in infected cells. Other fractions incorporated precursor only into replicative intermediate (RI) or replicative from (RF). Analysis of the product RF showed that it was of the same size and could bind to the same extent to oligo (dT)-cellulose as the RF isolated directly from lysates of infected cells. Displacement hybridization and ribonuclease digestion suggested that the purified polymerase could only complete previously initiated progeny positive strands using negative strands as template and, even in its most highly purified form, was still tightly bound to its template. Analysis on polyacrylamide slab gels revealed the presence of three 35S-labelled polypeptides in the purified polymerase preparation, but a polypeptide which had identical electrophoretic mobility to the lowest mol. wt. polypeptide of the purified polymerase was also present in material from mock-fected cells which had been taken through the purification procedure. From these results we conclude that only two virus-specified polypeptides are present in the polymerase. A scheme for the synthesis of these polypeptides is presented in the accompanying paper.", "contents": "Purification and polypeptide composition of Semliki Forest virus RNA polymerase. A purification method for Semliki Forest virus-specified RNA-dependent RNA polymerase from BHK cells is described. The procedure entails (i) the preparation of a crude cell lysate by Dounce homogenization of cells 3-5 h post-infection, (ii) differential centrifugation to give a 15 000 g 'mitochondrial' pellet, (iii) equilibrium centrifugation on discontinuous sucrose gradients (Friedman et al. 1972) to give a membranous band of density 1-16 g/ml, (iv) solubilization with Triton N-101 and velocity centrifugation to give a 25S solubilized polymerase complex and (v) affinity chromatography through an oligo (dT)-cellulose matrix bearing immobilized 42S virus particle RNA. The overall purification was approx. 360-fold with a 5% recovery of activity. Of the various intermediate fractions in the purfication procedure, only the relatively crude post-nuclear supernatant fraction was competent to synthesize the major single-stranded RNAs found in infected cells. Other fractions incorporated precursor only into replicative intermediate (RI) or replicative from (RF). Analysis of the product RF showed that it was of the same size and could bind to the same extent to oligo (dT)-cellulose as the RF isolated directly from lysates of infected cells. Displacement hybridization and ribonuclease digestion suggested that the purified polymerase could only complete previously initiated progeny positive strands using negative strands as template and, even in its most highly purified form, was still tightly bound to its template. Analysis on polyacrylamide slab gels revealed the presence of three 35S-labelled polypeptides in the purified polymerase preparation, but a polypeptide which had identical electrophoretic mobility to the lowest mol. wt. polypeptide of the purified polymerase was also present in material from mock-fected cells which had been taken through the purification procedure. From these results we conclude that only two virus-specified polypeptides are present in the polymerase. A scheme for the synthesis of these polypeptides is presented in the accompanying paper.", "PMID": 965947}
{"id": "pubmed23n0032_6518", "title": "RNA polymerase components in Semliki Forest virus-infected cells: synthesis from large precursors.", "content": "Two previously undescribed stable polypeptides (referred to as nsp 90 and nsp 63) appear in mammalian and avian cells infected with Semliki Forest virus. They are distinguishable from the virus structural proteins and their known precursors by their molecular weights and tryptic peptide maps, and are identical in size to two polypeptides found in purified preparations of virus-specific RNA polymerase. Data from pulse-chase experiments and from the use of inhibitors of proteolytic cleavage indicate that nsp 90 and nsp 63 are synthesized via a series of post-translational cleavages from three larger polypeptides, p200, p184 and p150. The labelling kinetics after synchronous initiation of protein synthesis are also consistent with the synthesis of nsp 90 and nsp 63 from a common initiation site, and show that nsp 63 is located close to this site. It is concluded that nsp 90 and nsp 63 are components of the virus-specific RNA polymerase, and are synthesized via a post-translational cleavage scheme entirely separate from that leading to the synthesis of the virus structural proteins.", "contents": "RNA polymerase components in Semliki Forest virus-infected cells: synthesis from large precursors. Two previously undescribed stable polypeptides (referred to as nsp 90 and nsp 63) appear in mammalian and avian cells infected with Semliki Forest virus. They are distinguishable from the virus structural proteins and their known precursors by their molecular weights and tryptic peptide maps, and are identical in size to two polypeptides found in purified preparations of virus-specific RNA polymerase. Data from pulse-chase experiments and from the use of inhibitors of proteolytic cleavage indicate that nsp 90 and nsp 63 are synthesized via a series of post-translational cleavages from three larger polypeptides, p200, p184 and p150. The labelling kinetics after synchronous initiation of protein synthesis are also consistent with the synthesis of nsp 90 and nsp 63 from a common initiation site, and show that nsp 63 is located close to this site. It is concluded that nsp 90 and nsp 63 are components of the virus-specific RNA polymerase, and are synthesized via a post-translational cleavage scheme entirely separate from that leading to the synthesis of the virus structural proteins.", "PMID": 965948}
{"id": "pubmed23n0032_6519", "title": "Purification and physicochemical characteristics of African swine fever virus.", "content": "Methods for the purification of African swine fever virus (ASFV) and its dissection into two fractions are described. The difficulties which have been encountered previously in attempts to purify the virus, namely contamination with large amounts of cellular constituents and aggregation of the virus particles, have been overcome by treatment with Tween 80 and by the use of 1 M-NaCl in the sucrose gradients. Five major polypeptides, mol. wt. 10(3) X 125 (VP1), 76 (VP2), 50 (VP3), 44 (VP4) and 39 (VP5) were found in the purified particles. The virus was dissected by treatment with Nonidet NP 40 into (a) a fraction which had the appearance of an empty capsid shell and capsid shell and contained the polypeptides VP2 and VP3 and (b) a structure containing VP1 and VP4. The location of VP5 was not ascertained.", "contents": "Purification and physicochemical characteristics of African swine fever virus. Methods for the purification of African swine fever virus (ASFV) and its dissection into two fractions are described. The difficulties which have been encountered previously in attempts to purify the virus, namely contamination with large amounts of cellular constituents and aggregation of the virus particles, have been overcome by treatment with Tween 80 and by the use of 1 M-NaCl in the sucrose gradients. Five major polypeptides, mol. wt. 10(3) X 125 (VP1), 76 (VP2), 50 (VP3), 44 (VP4) and 39 (VP5) were found in the purified particles. The virus was dissected by treatment with Nonidet NP 40 into (a) a fraction which had the appearance of an empty capsid shell and capsid shell and contained the polypeptides VP2 and VP3 and (b) a structure containing VP1 and VP4. The location of VP5 was not ascertained.", "PMID": 965949}
{"id": "pubmed23n0032_6520", "title": "[Contribution to the anatomoclinical and genetic study of tuberous sclerosis in infancy (author's transl)].", "content": "A case of Bourneville's disease is described, which is unusual because of the absence of clinical symptomatology until death occurred in the ninth month of life. Moreover, the post-mortem examination revealed the presence of cortical plaques of tuberous sclerosis which were in number, size and extension much more pronounced than in any previously published cases. On the basis of extensive topographical preparations of continuous sections taken from two cerebral hemispheres, the author provides an exact spatial representation of the tuberous sclerotic lesions and demonstrates the almost complete integrity of the central grey nuclei and the rhinencephalic complex. From data collected on numerous published infantile cases, in which he records the date of the onset of epilepsy, and, in addition, making use of neuropediatric and electrophysiological studies, he attempts to demonstrate that the appearance of the first neurological symptoms is related to certain stages of the cerebral corticalisation. So far as genetic factors are concerned, the family histroy did not provide any evidence of disease in ancestors, and a clinical examination of the parents did not disclose any stigmata of Bourneville's disease. This case can thus be considered as sporadic, due to a new mutation.", "contents": "[Contribution to the anatomoclinical and genetic study of tuberous sclerosis in infancy (author's transl)]. A case of Bourneville's disease is described, which is unusual because of the absence of clinical symptomatology until death occurred in the ninth month of life. Moreover, the post-mortem examination revealed the presence of cortical plaques of tuberous sclerosis which were in number, size and extension much more pronounced than in any previously published cases. On the basis of extensive topographical preparations of continuous sections taken from two cerebral hemispheres, the author provides an exact spatial representation of the tuberous sclerotic lesions and demonstrates the almost complete integrity of the central grey nuclei and the rhinencephalic complex. From data collected on numerous published infantile cases, in which he records the date of the onset of epilepsy, and, in addition, making use of neuropediatric and electrophysiological studies, he attempts to demonstrate that the appearance of the first neurological symptoms is related to certain stages of the cerebral corticalisation. So far as genetic factors are concerned, the family histroy did not provide any evidence of disease in ancestors, and a clinical examination of the parents did not disclose any stigmata of Bourneville's disease. This case can thus be considered as sporadic, due to a new mutation.", "PMID": 965950}
{"id": "pubmed23n0032_6521", "title": "[A genealogical study of Steinert's disease (author's transl)].", "content": "As a result of retrospective genealogical studies on 41 cases of myotonic dystrophy, the authors have found: 1) sound arguments to support the hypothesis of anticipation often discussed in association with this disease; 2) a high rate of mortinatality in the progeny of affected females; this aspect merits being taken into consideration in genetic counselling and should be reported to obstetricians. Its pathogenetic determination still remains obscure; 3) a relative frequency of illegitimacy in familes with affected fathers. On the basis of the low fertility associated with the disease, the authors postulate that new mutations may not be rare. Finally, they emphasize two little known symptomatic features, the first being the frequency of lithiasis, the second the frequency of neoplasms. This latter observation, however, still requires confirmation.", "contents": "[A genealogical study of Steinert's disease (author's transl)]. As a result of retrospective genealogical studies on 41 cases of myotonic dystrophy, the authors have found: 1) sound arguments to support the hypothesis of anticipation often discussed in association with this disease; 2) a high rate of mortinatality in the progeny of affected females; this aspect merits being taken into consideration in genetic counselling and should be reported to obstetricians. Its pathogenetic determination still remains obscure; 3) a relative frequency of illegitimacy in familes with affected fathers. On the basis of the low fertility associated with the disease, the authors postulate that new mutations may not be rare. Finally, they emphasize two little known symptomatic features, the first being the frequency of lithiasis, the second the frequency of neoplasms. This latter observation, however, still requires confirmation.", "PMID": 965951}
{"id": "pubmed23n0032_6522", "title": "Creatine-phosphokinase (CPK) activity in relatives of patients with X-linked muscular dystrophies: a Brazilian study.", "content": "Serum CPK was measured in 135 families with Duchenne muscular dystrophy (DMD) and 19 with the Becker type (BMD). Increased CPK was found in 62% of the carriers of DMD and 62.5% of the BMD. Two certain carries of DMD and one of their daughters showed clinical signs of myopathy. Three studied DMD pregnant carriers suggest that there is a decrease in CPK levels around the 4th-5th months of gestation. In genetic counselling of suspected carriers the CPK activity of their normal daughters should always be considered. Our data suggest strongly that CPK activity decreases in carriers with increasing age.", "contents": "Creatine-phosphokinase (CPK) activity in relatives of patients with X-linked muscular dystrophies: a Brazilian study. Serum CPK was measured in 135 families with Duchenne muscular dystrophy (DMD) and 19 with the Becker type (BMD). Increased CPK was found in 62% of the carriers of DMD and 62.5% of the BMD. Two certain carries of DMD and one of their daughters showed clinical signs of myopathy. Three studied DMD pregnant carriers suggest that there is a decrease in CPK levels around the 4th-5th months of gestation. In genetic counselling of suspected carriers the CPK activity of their normal daughters should always be considered. Our data suggest strongly that CPK activity decreases in carriers with increasing age.", "PMID": 965952}
{"id": "pubmed23n0032_6523", "title": "[A small supernumerary metacentric chromosome: interpr\u00e9tation test (author's transl)].", "content": "The report relates to a new case of small supernumerary metacentric chromosome. The phenotypical variability of previously published cases suggests that this chromosomal anomaly may arise from multiple origins. Our study shows the difficulties of a cytogenetic interpretation and the limits of the semeiologic arguments. It is, however, possible to hypothesize an 18 partial trisomy.", "contents": "[A small supernumerary metacentric chromosome: interpr\u00e9tation test (author's transl)]. The report relates to a new case of small supernumerary metacentric chromosome. The phenotypical variability of previously published cases suggests that this chromosomal anomaly may arise from multiple origins. Our study shows the difficulties of a cytogenetic interpretation and the limits of the semeiologic arguments. It is, however, possible to hypothesize an 18 partial trisomy.", "PMID": 965953}
{"id": "pubmed23n0032_6524", "title": "Comparative levels of creative ability among elementary school children.", "content": "Eighty-six black fourth-grade children and 111 white fourth-grade children randomly selected from normal classrooms in a rural country school system in Tennessee were compared on Torrance's Unusual Uses and Ask and Guess activities. No differences were found on the frequency or flexibility measures of either activity. No attempt was made to examine the results on any variable except race. Pearson Product Moment Correlations were computed on the Torrance Test results and Stanford-Binet intelligence test scores for both groups. The correlations were quite different with r = +.51 for the white students and r = +.22 for the black students.", "contents": "Comparative levels of creative ability among elementary school children. Eighty-six black fourth-grade children and 111 white fourth-grade children randomly selected from normal classrooms in a rural country school system in Tennessee were compared on Torrance's Unusual Uses and Ask and Guess activities. No differences were found on the frequency or flexibility measures of either activity. No attempt was made to examine the results on any variable except race. Pearson Product Moment Correlations were computed on the Torrance Test results and Stanford-Binet intelligence test scores for both groups. The correlations were quite different with r = +.51 for the white students and r = +.22 for the black students.", "PMID": 965954}
{"id": "pubmed23n0032_6525", "title": "An examination of errors in children's visual and haptic-tactual memory for random forms.", "content": "Hypotheses were advanced that (a) errors of omission would occur more frequently than errors of commission, and (b) errors would be related to stimulus complexity. Forty-eight children, 12 boys and 12 girls from each grades 1 and 3 were given a memory task for recognition of random polygons of 4, 8, 12, 16, and 20 sides; these forms were presented either visually or haptically. Visual memory was found to be superior to haptic memory, and performance on both tasks improved with age. Analyses of error-type and sideness, the two major variables of a priori interest, indicated support for both hypotheses. The curvilinear relationship between errors and sideness suggested difficulty in identifying shapes of intermediate complexity.", "contents": "An examination of errors in children's visual and haptic-tactual memory for random forms. Hypotheses were advanced that (a) errors of omission would occur more frequently than errors of commission, and (b) errors would be related to stimulus complexity. Forty-eight children, 12 boys and 12 girls from each grades 1 and 3 were given a memory task for recognition of random polygons of 4, 8, 12, 16, and 20 sides; these forms were presented either visually or haptically. Visual memory was found to be superior to haptic memory, and performance on both tasks improved with age. Analyses of error-type and sideness, the two major variables of a priori interest, indicated support for both hypotheses. The curvilinear relationship between errors and sideness suggested difficulty in identifying shapes of intermediate complexity.", "PMID": 965955}
{"id": "pubmed23n0032_6526", "title": "Empathy and pseudo-empathy: the affective judgments of first- and third-graders.", "content": "Eighty male and female first- and third-grader Ss were presented one of two brief videotaped social interaction episodes. Half of the Ss viewed an unambiguous tape in which a target character's affective response was congruent with the situational context in which he operated. The remaining Ss viewed a similar, but ambiguous tape in which the nonverbal affective response of the target character was incongruent with situational demands. Ss' responses were scored on the basis of their judgments of the target character's affective state and facial expression, and the degree of relative certainty with which they made their affective judgments. No differences were predicted or found in any of the empathic responses of first- and third-graders to the unambiguous tape. The two age groups studied did, however, differ significantly in their responses to the ambiguous tape. As anticipated, the first-grade Ss employed a highly centered inference strategy and based their judgments on only one of the available incongruous cues. The older Ss, by contrast, recognized the incongruities present and expressed little confidence in their ability to infer the true affect state of the target character.", "contents": "Empathy and pseudo-empathy: the affective judgments of first- and third-graders. Eighty male and female first- and third-grader Ss were presented one of two brief videotaped social interaction episodes. Half of the Ss viewed an unambiguous tape in which a target character's affective response was congruent with the situational context in which he operated. The remaining Ss viewed a similar, but ambiguous tape in which the nonverbal affective response of the target character was incongruent with situational demands. Ss' responses were scored on the basis of their judgments of the target character's affective state and facial expression, and the degree of relative certainty with which they made their affective judgments. No differences were predicted or found in any of the empathic responses of first- and third-graders to the unambiguous tape. The two age groups studied did, however, differ significantly in their responses to the ambiguous tape. As anticipated, the first-grade Ss employed a highly centered inference strategy and based their judgments on only one of the available incongruous cues. The older Ss, by contrast, recognized the incongruities present and expressed little confidence in their ability to infer the true affect state of the target character.", "PMID": 965956}
{"id": "pubmed23n0032_6527", "title": "Kinematics of locomotion by cats with a single hindlimb deafferented.", "content": "1. Cinematographic measurements were made of stepping by cats on a motor-driven treadmill, both normally and 2-3 wk after deafferentation of the LH (left hind) limb. 2. After surgery, rhythmic cycling of the LH limb was blurred whether the leg was dragged, as by some cats, or if it was lifted from the surface, as by others. 3. Interlimb coordination was also blurred with respect to normal, although distinct rhythms were still seen. The RH (right hind) limb descended prematurely and, in the walk, had a prolonged stance phase. The interval between touchdowns of hind- and forelimb on the left side no longer equaled that interval on the right side. 4. As is true for a normal cat, if the LH-deafferented animal stumbled, relatively normal single and interlimb cyclings were regained after several strides. 5. By kinematic analysis, force deficits were found in the deafferented LH limb both during the stance, when extensors should be most active, and the swing, when the limb failed to attain a normal position above the surface of the belt. Weight bearing by the LF (left fore) limb was altered in some animals. 6. At high speed, mean LH stance duration failed to decrease in the normal fashion. Inter-limb timings were reset to greater extent than in low-speed walking, as if the LH limb was being used only minimally. 7. It was concluded that both rhythm and force were impaired in the deafferented limb and also in the three intact limbs, whose weight bearing had to compensate for LH weakness. The changed mechanical demands after surgery were probably met by interactions between the remaining afferent input and central pattern generators so as to secure fairly effective and expedient locomotion.", "contents": "Kinematics of locomotion by cats with a single hindlimb deafferented. 1. Cinematographic measurements were made of stepping by cats on a motor-driven treadmill, both normally and 2-3 wk after deafferentation of the LH (left hind) limb. 2. After surgery, rhythmic cycling of the LH limb was blurred whether the leg was dragged, as by some cats, or if it was lifted from the surface, as by others. 3. Interlimb coordination was also blurred with respect to normal, although distinct rhythms were still seen. The RH (right hind) limb descended prematurely and, in the walk, had a prolonged stance phase. The interval between touchdowns of hind- and forelimb on the left side no longer equaled that interval on the right side. 4. As is true for a normal cat, if the LH-deafferented animal stumbled, relatively normal single and interlimb cyclings were regained after several strides. 5. By kinematic analysis, force deficits were found in the deafferented LH limb both during the stance, when extensors should be most active, and the swing, when the limb failed to attain a normal position above the surface of the belt. Weight bearing by the LF (left fore) limb was altered in some animals. 6. At high speed, mean LH stance duration failed to decrease in the normal fashion. Inter-limb timings were reset to greater extent than in low-speed walking, as if the LH limb was being used only minimally. 7. It was concluded that both rhythm and force were impaired in the deafferented limb and also in the three intact limbs, whose weight bearing had to compensate for LH weakness. The changed mechanical demands after surgery were probably met by interactions between the remaining afferent input and central pattern generators so as to secure fairly effective and expedient locomotion.", "PMID": 966036}
{"id": "pubmed23n0032_6528", "title": "Responses of cells of posterior lateral line lobe to activation of electroreceptors in a mormyrid fish.", "content": "Activity of neurons in the lateral line lobe was studied by intracellular recording of responses to stimulation of the lateral line nerves and of electroreceptors on the skin surface. Two modes of activation occur for cells responding to inputs from medium receptors. There is a direct monosynaptic input mediated by a single fiber. Short latency of response and antidromic spread from cell to afferent fiber indicate that the mediating synapse is electrotonic. The second input is from a number of additional fibers and is relayed, presumably by the granule cells. At shortest latency this input is disynaptic, probably involving at least one electrotonic synapse. A relay is indicated by heterosynaptic facilitation of the PSP and by pronounced depression with repetitive stimulation. The monosynaptic input may be on the axon. Disynaptic inputs are distributed over the dendrites, and impulses can arise in the dendrites. What appear to be spikes restricted to dendritic regions are often recorded as small brief potentials in the cell body. There is a somatotopic projection of the electroreceptors to the lateral line lobe. The monosynaptic input comes from a specific receptor in the periphery. Strong disynaptic inputs come from a group of receptors generally found anterior, but less commonly posterior or lateral, to the receptor giving rise to the monosynaptic input. Additional inputs that are inhibitory come from surrounding receptors. The inhibition only affects responses to the disynaptic input. The different inputs and multiple sites of impulse initiation must modify the cell's response as compared with the input-output relations that would be obtained with inputs acting on a single summation point. Cells responding to activation of large receptors are infrequent. They are characterized by low threshold, little latency change near threshold, and ability to follow high frequencies of stimulation.", "contents": "Responses of cells of posterior lateral line lobe to activation of electroreceptors in a mormyrid fish. Activity of neurons in the lateral line lobe was studied by intracellular recording of responses to stimulation of the lateral line nerves and of electroreceptors on the skin surface. Two modes of activation occur for cells responding to inputs from medium receptors. There is a direct monosynaptic input mediated by a single fiber. Short latency of response and antidromic spread from cell to afferent fiber indicate that the mediating synapse is electrotonic. The second input is from a number of additional fibers and is relayed, presumably by the granule cells. At shortest latency this input is disynaptic, probably involving at least one electrotonic synapse. A relay is indicated by heterosynaptic facilitation of the PSP and by pronounced depression with repetitive stimulation. The monosynaptic input may be on the axon. Disynaptic inputs are distributed over the dendrites, and impulses can arise in the dendrites. What appear to be spikes restricted to dendritic regions are often recorded as small brief potentials in the cell body. There is a somatotopic projection of the electroreceptors to the lateral line lobe. The monosynaptic input comes from a specific receptor in the periphery. Strong disynaptic inputs come from a group of receptors generally found anterior, but less commonly posterior or lateral, to the receptor giving rise to the monosynaptic input. Additional inputs that are inhibitory come from surrounding receptors. The inhibition only affects responses to the disynaptic input. The different inputs and multiple sites of impulse initiation must modify the cell's response as compared with the input-output relations that would be obtained with inputs acting on a single summation point. Cells responding to activation of large receptors are infrequent. They are characterized by low threshold, little latency change near threshold, and ability to follow high frequencies of stimulation.", "PMID": 966037}
{"id": "pubmed23n0032_6529", "title": "Frequency response of human soleus muscle.", "content": "1. The properties of human soleus muscle were studied by systems analysis. Single stimulus pulses and random stimulus pulse trains were applied to a branch of the nerve to soleus muscle and the resultant tension fluctuations were recorded. 2. The frequency-response function between stimulus pulses and tension conforms to that of a second-order, low-pass filter. The parameters of the second-order system, low frequency gain, natural frequency, and damping ratio, varied systematically with the angle of the ankle. As the ankle was flexed (the length of the muscle was increased), the low frequency gain increased, the natural frequency decreased, and the damping ratio was unaffected or increased slightly. 3. These results are discussed in relation to the twitch responses of human soleus muscles and the responses previously observed in cat muscles.", "contents": "Frequency response of human soleus muscle. 1. The properties of human soleus muscle were studied by systems analysis. Single stimulus pulses and random stimulus pulse trains were applied to a branch of the nerve to soleus muscle and the resultant tension fluctuations were recorded. 2. The frequency-response function between stimulus pulses and tension conforms to that of a second-order, low-pass filter. The parameters of the second-order system, low frequency gain, natural frequency, and damping ratio, varied systematically with the angle of the ankle. As the ankle was flexed (the length of the muscle was increased), the low frequency gain increased, the natural frequency decreased, and the damping ratio was unaffected or increased slightly. 3. These results are discussed in relation to the twitch responses of human soleus muscles and the responses previously observed in cat muscles.", "PMID": 966038}
{"id": "pubmed23n0032_6530", "title": "Axon conduction block in a region of dense connective tissue in crayfish.", "content": "1. The excitor nerve which innervates the opener muscle of the crayfish walking leg was stimulated repetitively for prolonged periods of time. 2. After an initial facilitation and subsequent depression of the postsynaptic potentials, occasional stimulus pulses failed to evoke a postsynaptic response; eventually, every second stimulus pulse failed to produce a postsynaptic potential change. This is termed the 1:1 block state. 3. The block resulted from failure of the appropriate nerve action potentials to propagate through an identifiable region of the axon. This region was in the joint between the propodite and the carpopodite and was characterized by dense surrounding connective tissue. 4. Associated with block development is a decrease in axon conduction velocity and an increase in the amplitude of the postsynaptic potentials; this latter phenomenon is termed anomalous facilitation and results from a decrease in the extent of synaptic depression. 5. The cause of the conduction block resides in the associated nonneural tissue and not in some geometrical property of the axon. 6. It is concluded that extracellular accumulation of K+, because of limited diffusion possibilities and possibly reduced Schwann cell tissue, depolarizes the axon, leading to propagation failure.", "contents": "Axon conduction block in a region of dense connective tissue in crayfish. 1. The excitor nerve which innervates the opener muscle of the crayfish walking leg was stimulated repetitively for prolonged periods of time. 2. After an initial facilitation and subsequent depression of the postsynaptic potentials, occasional stimulus pulses failed to evoke a postsynaptic response; eventually, every second stimulus pulse failed to produce a postsynaptic potential change. This is termed the 1:1 block state. 3. The block resulted from failure of the appropriate nerve action potentials to propagate through an identifiable region of the axon. This region was in the joint between the propodite and the carpopodite and was characterized by dense surrounding connective tissue. 4. Associated with block development is a decrease in axon conduction velocity and an increase in the amplitude of the postsynaptic potentials; this latter phenomenon is termed anomalous facilitation and results from a decrease in the extent of synaptic depression. 5. The cause of the conduction block resides in the associated nonneural tissue and not in some geometrical property of the axon. 6. It is concluded that extracellular accumulation of K+, because of limited diffusion possibilities and possibly reduced Schwann cell tissue, depolarizes the axon, leading to propagation failure.", "PMID": 966039}
{"id": "pubmed23n0032_6531", "title": "Mechanoreceptors for near-field water displacements in crayfish.", "content": "1. Mechanosensory hairs on the surface of the crayfish telson are dually innervated, one sensory cell responding to headward, the other to tailward deflection of the hair. The average conduction velocity of headward elements was 0.8 m/s (variance 0.08) and of tailward elements 1.2 m/s (variance 0.19). In a frequency range from 0.05 to 200 Hz, thresholds were lowest near 20 Hz: 0.08 mum (pp) for headward-sensitive and 0.1 mum (pp) for tailward-sensitive cells. 2. The receptors are displacement sensitive since thresholds are of the same order of magnitude over the frequency range 1-70 Hz when the hair is moved by a vibrating wire loop. With natural stimuli (surface waves), the velocity component of the particle movement (and consequently force) becomes influential. The coding of a broad range of stimulus intensities is aided by variations in mechanical properties of the hair. 3. Marked directionality (better than 4:1), in addition to the dual innervation, enhances vector detection. At least part of this characteristic stems from the hingelike articulation of the hair on the body surface: the hair can be moved easily 40 degrees tailward and 20 degrees headward, but must be forced in the orthogonal direction. Morphological studies indicate the presence of a double pivoted hinge, with rigid guides for movement of the hair shaft. Preliminary results of electron microscope examination show a clearly polarized arrangement of densely packed microtubules in the two dendrites; they appear interconnected in groups of two and three along a line parallel to the sensitivity plane of the receptor. 4. The 50-fold threshold difference between the results of behavioral experiments in lobsters (24) and the data for the individual receptors reported here may be due to improvement in signal-to-noise ratio by central nervous averaging of  the input from an estimated 2 X 10(3) receptors (Procambarus), and/or to the kind of threshold criteria applied to individual receptor thresholds. As would be expected (35), the sensory cells of each directional class synapse with separate interneurons: in this way, the organism might employ differential microphones to reduce background noise. 5. The receptors are analogous to those of the lateral-line system in lower vertebrates in having receptors with sensitivities polarized by 180 degrees. These similarities suggest that in both cases monitoring of near field water displacements has proved in essential way of orienting in opaque waters.", "contents": "Mechanoreceptors for near-field water displacements in crayfish. 1. Mechanosensory hairs on the surface of the crayfish telson are dually innervated, one sensory cell responding to headward, the other to tailward deflection of the hair. The average conduction velocity of headward elements was 0.8 m/s (variance 0.08) and of tailward elements 1.2 m/s (variance 0.19). In a frequency range from 0.05 to 200 Hz, thresholds were lowest near 20 Hz: 0.08 mum (pp) for headward-sensitive and 0.1 mum (pp) for tailward-sensitive cells. 2. The receptors are displacement sensitive since thresholds are of the same order of magnitude over the frequency range 1-70 Hz when the hair is moved by a vibrating wire loop. With natural stimuli (surface waves), the velocity component of the particle movement (and consequently force) becomes influential. The coding of a broad range of stimulus intensities is aided by variations in mechanical properties of the hair. 3. Marked directionality (better than 4:1), in addition to the dual innervation, enhances vector detection. At least part of this characteristic stems from the hingelike articulation of the hair on the body surface: the hair can be moved easily 40 degrees tailward and 20 degrees headward, but must be forced in the orthogonal direction. Morphological studies indicate the presence of a double pivoted hinge, with rigid guides for movement of the hair shaft. Preliminary results of electron microscope examination show a clearly polarized arrangement of densely packed microtubules in the two dendrites; they appear interconnected in groups of two and three along a line parallel to the sensitivity plane of the receptor. 4. The 50-fold threshold difference between the results of behavioral experiments in lobsters (24) and the data for the individual receptors reported here may be due to improvement in signal-to-noise ratio by central nervous averaging of  the input from an estimated 2 X 10(3) receptors (Procambarus), and/or to the kind of threshold criteria applied to individual receptor thresholds. As would be expected (35), the sensory cells of each directional class synapse with separate interneurons: in this way, the organism might employ differential microphones to reduce background noise. 5. The receptors are analogous to those of the lateral-line system in lower vertebrates in having receptors with sensitivities polarized by 180 degrees. These similarities suggest that in both cases monitoring of near field water displacements has proved in essential way of orienting in opaque waters.", "PMID": 966040}
{"id": "pubmed23n0032_6532", "title": "Integration of directional mechanosensory input by crayfish interneurons.", "content": "1. Interneurons activated by mechanosensory hairs on the crayfish telson respond selectively to directional displacements of the medium; the directions of maximum sensitivity lie 180 degrees apart in approximately the rostrocaudal plane, corresponding to the directional sensitivities of the two populations of primary afferent neurons. We have examined the basis for this selectivity by intracellular recording in the interneurons, correlating subthreshold potentials with activity evoked in identified afferents by bending single hairs or by producing nearfield displacements of the medium. 2. Interneurons can usually be caused to discharge by a brief train of impulses in single sensory axons. Unitary EPEPs are associated with arriving affterent spikes in the fourth (sensory) root; each primary interneuron receives convergence from several sensory axons, all sensitive to the same direction of movement. Since each afferent axon is drawn from a pair innervating a single sensory structure, this remarkable specificity of connection is unlikely to depend on an anatomical mode of address. 3. Higher order interneurons receive from directionally sensitive lower order interneurons of the same class, as well as from primary afferents of that class. The responses of such cells may show much more decrement during a train of displacement stimuli than do those of lower order cells. Directionality does not appear to be enhanced. 4. During the \"null phase\" some interneurons appear to be actively inhibited: bending of single hairs 180 degrees away from the effective direction may produce membrane hyperpolarization and slow spontaneous discharges, and shocks to afferent roots produce mixtures of monosynaptic EPSPs and polysynaptic IPSPs.", "contents": "Integration of directional mechanosensory input by crayfish interneurons. 1. Interneurons activated by mechanosensory hairs on the crayfish telson respond selectively to directional displacements of the medium; the directions of maximum sensitivity lie 180 degrees apart in approximately the rostrocaudal plane, corresponding to the directional sensitivities of the two populations of primary afferent neurons. We have examined the basis for this selectivity by intracellular recording in the interneurons, correlating subthreshold potentials with activity evoked in identified afferents by bending single hairs or by producing nearfield displacements of the medium. 2. Interneurons can usually be caused to discharge by a brief train of impulses in single sensory axons. Unitary EPEPs are associated with arriving affterent spikes in the fourth (sensory) root; each primary interneuron receives convergence from several sensory axons, all sensitive to the same direction of movement. Since each afferent axon is drawn from a pair innervating a single sensory structure, this remarkable specificity of connection is unlikely to depend on an anatomical mode of address. 3. Higher order interneurons receive from directionally sensitive lower order interneurons of the same class, as well as from primary afferents of that class. The responses of such cells may show much more decrement during a train of displacement stimuli than do those of lower order cells. Directionality does not appear to be enhanced. 4. During the \"null phase\" some interneurons appear to be actively inhibited: bending of single hairs 180 degrees away from the effective direction may produce membrane hyperpolarization and slow spontaneous discharges, and shocks to afferent roots produce mixtures of monosynaptic EPSPs and polysynaptic IPSPs.", "PMID": 966041}
{"id": "pubmed23n0032_6533", "title": "Electrical measurement of axon diameter and its use in relating motoneuron size to critical firing level.", "content": "1. Action potentials of single plantaris motoneurons were recorded monophasically from fine ventral root filaments. A resistor was placed in shunt across the recording electrodes and its value was varied until the size of the action potentials was reduced by one-half. At this point the resistance of the filament was taken to be equal to that of the shunt, and the quotient of action-potential amplitude divided by filament resistance was proportional to the axonal action current. 2. The measurement of axonal action currents depends on the assumption that the filament-electrode system obeys Ohm's law. Tests were performed which validated this assumption. It was then shown that the axonal action currents varied as the square of conduction velocity over the range of alpha and gamma motoneurons. 3. A direct correlation was established between the critical firing levels of motoneurons and the sizes of the impulses in their axons after the recorded sizes had been normalized in accordance with the resistances of the ventral root filaments in which they were located. Since both the CFL and axonal diameter were related to impulse size, they were related to each other (Fig. 6). 4. Evidence is cited justifying the conclusion that the dimater of a motor axon is directly related to the size of its soma. Thus, it may be inferred that the critical firing level of a motoneuron is a function of its size.", "contents": "Electrical measurement of axon diameter and its use in relating motoneuron size to critical firing level. 1. Action potentials of single plantaris motoneurons were recorded monophasically from fine ventral root filaments. A resistor was placed in shunt across the recording electrodes and its value was varied until the size of the action potentials was reduced by one-half. At this point the resistance of the filament was taken to be equal to that of the shunt, and the quotient of action-potential amplitude divided by filament resistance was proportional to the axonal action current. 2. The measurement of axonal action currents depends on the assumption that the filament-electrode system obeys Ohm's law. Tests were performed which validated this assumption. It was then shown that the axonal action currents varied as the square of conduction velocity over the range of alpha and gamma motoneurons. 3. A direct correlation was established between the critical firing levels of motoneurons and the sizes of the impulses in their axons after the recorded sizes had been normalized in accordance with the resistances of the ventral root filaments in which they were located. Since both the CFL and axonal diameter were related to impulse size, they were related to each other (Fig. 6). 4. Evidence is cited justifying the conclusion that the dimater of a motor axon is directly related to the size of its soma. Thus, it may be inferred that the critical firing level of a motoneuron is a function of its size.", "PMID": 966042}
{"id": "pubmed23n0032_6534", "title": "Modulation of spike frequency by regions of special axonal geometry and by synaptic inputs.", "content": "1. Spike propagation across the nonhomogeneous section of the giant axon in ganglion T3 of the cockroach was analyzed by intracellular microelectrodes recording at the posterior and anterior ends of T3. Ascending and descending potentials were evoked by stimulation of A5-A6 and T2-T3 connectives. 2. At high frequencies, descending and ascending impulses exhibit the following: a) consecutive reduction in the spike amplitude, b) a decrease in the afterhyperpolarization; c) gradual appearance of a prepotential together with an increase in delay of spike initiation; d) failure of full spike invasion into the recording area, showing only a decremental potential. 3. The duration of a train required to block spike propagation when the whole connective is stimulated is much shorter (about 6 times) than that required when a single giant axon is stimulated. 4. The conduction block is associated with a marked decrease in effective membrane resistance, greater than that expected from depolarization and delayed rectification. 5. Synaptic potentials could be recorded in the giant axons in the caudal base of ganglion T3 after stimulation of either the ipsilateral or contralateral connectives at both ends of the ganglion. These synaptic potentials could be blocked by d-tubocurarine (d-TC) or low Ca2+-high Mg2+. 6. Activation of these synapses produces a marked increase in membrane conductance, blocking propagation of spike trains through the ganglion. 7. After these synapses are blocked by d-TC or low Ca2+-high Mg2+, high-frequency stimulation still produces a conduction block. 8. It seems that conduction of spike during repetitive stimulation is affected both by accumulation of extracellular potassium, which depolarizes the membrane and causes sodium inactivation, and by activation of synaptic inputs to shunt the membrane in this region. 8. Each of these two mechanisms by itself can produce conduction block along the giant axons in ganglion T3.", "contents": "Modulation of spike frequency by regions of special axonal geometry and by synaptic inputs. 1. Spike propagation across the nonhomogeneous section of the giant axon in ganglion T3 of the cockroach was analyzed by intracellular microelectrodes recording at the posterior and anterior ends of T3. Ascending and descending potentials were evoked by stimulation of A5-A6 and T2-T3 connectives. 2. At high frequencies, descending and ascending impulses exhibit the following: a) consecutive reduction in the spike amplitude, b) a decrease in the afterhyperpolarization; c) gradual appearance of a prepotential together with an increase in delay of spike initiation; d) failure of full spike invasion into the recording area, showing only a decremental potential. 3. The duration of a train required to block spike propagation when the whole connective is stimulated is much shorter (about 6 times) than that required when a single giant axon is stimulated. 4. The conduction block is associated with a marked decrease in effective membrane resistance, greater than that expected from depolarization and delayed rectification. 5. Synaptic potentials could be recorded in the giant axons in the caudal base of ganglion T3 after stimulation of either the ipsilateral or contralateral connectives at both ends of the ganglion. These synaptic potentials could be blocked by d-tubocurarine (d-TC) or low Ca2+-high Mg2+. 6. Activation of these synapses produces a marked increase in membrane conductance, blocking propagation of spike trains through the ganglion. 7. After these synapses are blocked by d-TC or low Ca2+-high Mg2+, high-frequency stimulation still produces a conduction block. 8. It seems that conduction of spike during repetitive stimulation is affected both by accumulation of extracellular potassium, which depolarizes the membrane and causes sodium inactivation, and by activation of synaptic inputs to shunt the membrane in this region. 8. Each of these two mechanisms by itself can produce conduction block along the giant axons in ganglion T3.", "PMID": 966043}
{"id": "pubmed23n0032_6535", "title": "Ultrastructure of region of a low safety factor in inhomogeneous giant axon of the cockroach.", "content": "1. The structure of the ventral giant axons of the cockroach at the level of ganglion T3 was studied by means of light and electron microscopy. 2. From serial sections and cobalt injections, the axons diameter was found to range between 40 and 60 mum at the caudal end of ganglion T3; toward the center of T3 they narrow to 20-40 mum, and again expand to 30-45 mum anteriorly in ganglion T3. 3. Each giant axon sends off several branches, 1-15 mum in diameter, into the neuropil. The giant axons and the bases of their branches are enveloped by cytoplasmic processes of glial cells. The periaxonal space is about 100-200 A. 4. Distally the branches are devoid of glial envelopes and the extracellular space between the branches and other axonal profiles is about 200 A. Terminals with presumptive chemical synapses on the giant axon branches were found. Clear vesicles, 300-400 A in diameter, are seen clustered together. The width of the supposedly synaptic gap is about 100 A. 5. In some areas the branches and other axonal profiles form close appositions.", "contents": "Ultrastructure of region of a low safety factor in inhomogeneous giant axon of the cockroach. 1. The structure of the ventral giant axons of the cockroach at the level of ganglion T3 was studied by means of light and electron microscopy. 2. From serial sections and cobalt injections, the axons diameter was found to range between 40 and 60 mum at the caudal end of ganglion T3; toward the center of T3 they narrow to 20-40 mum, and again expand to 30-45 mum anteriorly in ganglion T3. 3. Each giant axon sends off several branches, 1-15 mum in diameter, into the neuropil. The giant axons and the bases of their branches are enveloped by cytoplasmic processes of glial cells. The periaxonal space is about 100-200 A. 4. Distally the branches are devoid of glial envelopes and the extracellular space between the branches and other axonal profiles is about 200 A. Terminals with presumptive chemical synapses on the giant axon branches were found. Clear vesicles, 300-400 A in diameter, are seen clustered together. The width of the supposedly synaptic gap is about 100 A. 5. In some areas the branches and other axonal profiles form close appositions.", "PMID": 966044}
{"id": "pubmed23n0032_6536", "title": "Theoretical analysis of parameters leading to frequency modulation along an inhomogeneous axon.", "content": "1. Theoretical computations were conducted on a computer model of a segmented, nonhomogeneous axon to understand the mechanism of frequency block of conduction. 2. The model is based on the Hodgkin-Huxley equations modified in several ways to better describe the cockroach axon. We used cockroach parameters where available. 3. The increase in fiber radius was spread over a series of segments to approximate a taper. We found that a taper allows a larger overall increase in fiber diameter than a single step to be successfully passed. 4. We studied effects on a train of impulses. The modified equations included effects due to changes in extracellular potassium concentration resulting from the repetitive firing of the axon. 5. An increase in diameter which allows a single spike to pass blocks the subsequent impulses in a train at the taper if potassium concentration variability is introduced. This could explain the low-pass filter characteristics of axon constrictions. 6. Results of the model fit well with the experiemental spike shape and height. Data were computed for the refractory period and its dependence on the taper parameters.", "contents": "Theoretical analysis of parameters leading to frequency modulation along an inhomogeneous axon. 1. Theoretical computations were conducted on a computer model of a segmented, nonhomogeneous axon to understand the mechanism of frequency block of conduction. 2. The model is based on the Hodgkin-Huxley equations modified in several ways to better describe the cockroach axon. We used cockroach parameters where available. 3. The increase in fiber radius was spread over a series of segments to approximate a taper. We found that a taper allows a larger overall increase in fiber diameter than a single step to be successfully passed. 4. We studied effects on a train of impulses. The modified equations included effects due to changes in extracellular potassium concentration resulting from the repetitive firing of the axon. 5. An increase in diameter which allows a single spike to pass blocks the subsequent impulses in a train at the taper if potassium concentration variability is introduced. This could explain the low-pass filter characteristics of axon constrictions. 6. Results of the model fit well with the experiemental spike shape and height. Data were computed for the refractory period and its dependence on the taper parameters.", "PMID": 966045}
{"id": "pubmed23n0032_6537", "title": "Imaging small pulmonary ischemic lesions after radioactive carbon monoxide inhalation.", "content": "A new method is described for imaging small ischemic regions in the lung immediately after a single breath of radioactive carbon monoxide (11CO). A tungsten-collimated scintillation camera is used to visualize the  0.51-MeV annihilation photons due to the 11C. In normal dogs the entire field is cleared of 11CO within 10 sec. However, in dogs with experimentally occluded 2-mm-diam segmental arteries, the ischemic but well-ventilated segment appears as a region of persistent high radioactivity, due most likely to temporary entrapment of 11CO-labeled red blood cells in the ischemic region. This technique also provides a simple noninvasive means for instantly labeling the systemic circulation without left heart catheterization.", "contents": "Imaging small pulmonary ischemic lesions after radioactive carbon monoxide inhalation. A new method is described for imaging small ischemic regions in the lung immediately after a single breath of radioactive carbon monoxide (11CO). A tungsten-collimated scintillation camera is used to visualize the  0.51-MeV annihilation photons due to the 11C. In normal dogs the entire field is cleared of 11CO within 10 sec. However, in dogs with experimentally occluded 2-mm-diam segmental arteries, the ischemic but well-ventilated segment appears as a region of persistent high radioactivity, due most likely to temporary entrapment of 11CO-labeled red blood cells in the ischemic region. This technique also provides a simple noninvasive means for instantly labeling the systemic circulation without left heart catheterization.", "PMID": 966052}
{"id": "pubmed23n0032_6538", "title": "Significance of delayed 67Ga localization in the kidneys.", "content": "One hundred and seventy-five 67Ga-citrate scans were performed to detect suspected occult inflammatory processes. None of the patients had a known malignancy. Renal activity was noted in 12 patients (6.8%) on the 48-hr image. These patients had either pyelonephritis, acute tubular necrosis, vasculitis, or a renal abscess. Since delayed 67Ga uptake in the kidneys may be the first evidence of renal disease, further investigation, including either arteriography or biopsy, is necessary. In patients with a known malignancy, tumor involvement must be considered.", "contents": "Significance of delayed 67Ga localization in the kidneys. One hundred and seventy-five 67Ga-citrate scans were performed to detect suspected occult inflammatory processes. None of the patients had a known malignancy. Renal activity was noted in 12 patients (6.8%) on the 48-hr image. These patients had either pyelonephritis, acute tubular necrosis, vasculitis, or a renal abscess. Since delayed 67Ga uptake in the kidneys may be the first evidence of renal disease, further investigation, including either arteriography or biopsy, is necessary. In patients with a known malignancy, tumor involvement must be considered.", "PMID": 966053}
{"id": "pubmed23n0032_6539", "title": "A simple technique for measuring relative renal blood flow.", "content": "To determine whether externally monitored early renal uptake of 131I-hippurate is proportional to renal blood flow, the renal uptake of 131I-hippurate at 1--2 min after injection was compared with the renal accumulation of radioactive carbonized microspheres in dogs. A renal artery catheter equipped with a balloon was used to decrease renal blood flow unilaterally. One minute after the intravenous injection of 100 muCi of 131I-hippurate, about 1 muCi of either  85Sr- or 98Nb-labeled carbon microspheres was injected into the left ventricle. Radioactivity was measured over both kidneys. The total radioactivity within each kidney region of interest was corrected for background and integrated over the 1--2 min after injection. Thirteen measurements of relative renal blood flow were made for seven dogs. The dogs were then killed and both kidneys were excised and counted for the radioactivity of the microspheres. The 1--2-min relative renal uptake of 131I-hippurate correlated well with relative microsphere uptake, suggesting that relative renal blood flow can be simply determined from the external measurement of renal uptake of 131I-hippurate.", "contents": "A simple technique for measuring relative renal blood flow. To determine whether externally monitored early renal uptake of 131I-hippurate is proportional to renal blood flow, the renal uptake of 131I-hippurate at 1--2 min after injection was compared with the renal accumulation of radioactive carbonized microspheres in dogs. A renal artery catheter equipped with a balloon was used to decrease renal blood flow unilaterally. One minute after the intravenous injection of 100 muCi of 131I-hippurate, about 1 muCi of either  85Sr- or 98Nb-labeled carbon microspheres was injected into the left ventricle. Radioactivity was measured over both kidneys. The total radioactivity within each kidney region of interest was corrected for background and integrated over the 1--2 min after injection. Thirteen measurements of relative renal blood flow were made for seven dogs. The dogs were then killed and both kidneys were excised and counted for the radioactivity of the microspheres. The 1--2-min relative renal uptake of 131I-hippurate correlated well with relative microsphere uptake, suggesting that relative renal blood flow can be simply determined from the external measurement of renal uptake of 131I-hippurate.", "PMID": 966054}
{"id": "pubmed23n0032_6540", "title": "Intercomparison of myocardial imaging agents: 201Ti, 129Cs, 43K, and 81Rb.", "content": "Thallium-201, 129Cs, 43K, and 81Rb were evaluated as \"static\" myocardial-imaging agents. Optimal settings of the scintillation camera were determined for each agent. Accumulation for good-quality images can be started as early as 5 min after the dose with 43K, 10 min with 201T1, and 30 min with 129Cs. Imaging times were comparable for 43K, 129Cs, and 201T1 (using the 80-keV x-rays). High-energy photons from the 81Rb preparation, largely from 82mRb contaminant, made it impossible to obtain an interpretable image without the addition of more shielding. Absorbed radiation dose from 81Rb is lower than that from 43K, 129Cs, and 201T1. The highest background activity was observed with 81Rb, followed by 43K, 129Cs, and 201T1 in that order. Overall, 201T1 was best suited for imaging acute myocardial infarction with currently available equipment, and 129Cs was next best. However, because of instrument setting and commercially obtained preparations, 81Rb could not be properly compared with the other radionuclides in our study.", "contents": "Intercomparison of myocardial imaging agents: 201Ti, 129Cs, 43K, and 81Rb. Thallium-201, 129Cs, 43K, and 81Rb were evaluated as \"static\" myocardial-imaging agents. Optimal settings of the scintillation camera were determined for each agent. Accumulation for good-quality images can be started as early as 5 min after the dose with 43K, 10 min with 201T1, and 30 min with 129Cs. Imaging times were comparable for 43K, 129Cs, and 201T1 (using the 80-keV x-rays). High-energy photons from the 81Rb preparation, largely from 82mRb contaminant, made it impossible to obtain an interpretable image without the addition of more shielding. Absorbed radiation dose from 81Rb is lower than that from 43K, 129Cs, and 201T1. The highest background activity was observed with 81Rb, followed by 43K, 129Cs, and 201T1 in that order. Overall, 201T1 was best suited for imaging acute myocardial infarction with currently available equipment, and 129Cs was next best. However, because of instrument setting and commercially obtained preparations, 81Rb could not be properly compared with the other radionuclides in our study.", "PMID": 966055}
{"id": "pubmed23n0032_6541", "title": "Right atrial myxoma presenting as nonresolving pulmonary emboli: case report.", "content": "A case of right atrial myxoma causing extensive pulmonary embolization is described. Five years elapsed between the initial consultation and the correct diagnosis. The patient has been free of symptoms for 3 years following surgical removal of the tumor. The clinical manifestations of right atrial myxoma, the differential diagnosis, and the atypical lung-scan features are discussed.", "contents": "Right atrial myxoma presenting as nonresolving pulmonary emboli: case report. A case of right atrial myxoma causing extensive pulmonary embolization is described. Five years elapsed between the initial consultation and the correct diagnosis. The patient has been free of symptoms for 3 years following surgical removal of the tumor. The clinical manifestations of right atrial myxoma, the differential diagnosis, and the atypical lung-scan features are discussed.", "PMID": 966056}
{"id": "pubmed23n0032_6542", "title": "Gallium imaging in pulmonary artery sarcoma mimicking pulmonary embolism: case report.", "content": "Primary pulmonary artery sarcoma provides perfusion-ventilation images, as well as arteriographic studies, that can suggest pulmonary embolism. The awareness of atypical correlation among the studies for pulmonary embolism can lead to an early suspicion of pulmonary artery tumor. Imaging with 67Ga-citrate may facilitate earlier diagnosis.", "contents": "Gallium imaging in pulmonary artery sarcoma mimicking pulmonary embolism: case report. Primary pulmonary artery sarcoma provides perfusion-ventilation images, as well as arteriographic studies, that can suggest pulmonary embolism. The awareness of atypical correlation among the studies for pulmonary embolism can lead to an early suspicion of pulmonary artery tumor. Imaging with 67Ga-citrate may facilitate earlier diagnosis.", "PMID": 966057}
{"id": "pubmed23n0032_6543", "title": "Intense myocardial uptake of 99mTc-diphosphonate in a uremic patient with secondary hyperparathyroidism and pericarditis: case report.", "content": "A patient on chronic hemodialysis, with secondary hyperparathyroidism and uremic pericarditis, showed marked myocardial uptake of 99mTc-diphosphonate. Several possible mechanisms for the myocardial uptake are discussed.", "contents": "Intense myocardial uptake of 99mTc-diphosphonate in a uremic patient with secondary hyperparathyroidism and pericarditis: case report. A patient on chronic hemodialysis, with secondary hyperparathyroidism and uremic pericarditis, showed marked myocardial uptake of 99mTc-diphosphonate. Several possible mechanisms for the myocardial uptake are discussed.", "PMID": 966058}
{"id": "pubmed23n0032_6544", "title": "Scan findings in a case of splenic infarction due to amyloidosis: case report.", "content": "Spleen images in a patient with monoclonal gammopathy showed numerous areas of decreased radiocolloid accumulation. Microscopic examination revealed areas of coagulation necrosis with diffuse amyloid infiltration in the spleen. In this patient, amyloidosis is considered to have caused the infarct.", "contents": "Scan findings in a case of splenic infarction due to amyloidosis: case report. Spleen images in a patient with monoclonal gammopathy showed numerous areas of decreased radiocolloid accumulation. Microscopic examination revealed areas of coagulation necrosis with diffuse amyloid infiltration in the spleen. In this patient, amyloidosis is considered to have caused the infarct.", "PMID": 966059}
{"id": "pubmed23n0032_6545", "title": "Scintigraphic detection of hepatic metastases with 131I-labeled steroid in recurrent adrenal carcinoma: case report.", "content": "Abdominal scintigraphy using a new 131I-labeled steroid agent was performed on a 40-year-old women proven by surgery to have adrenocortical carcinoma. Considerable accumulations were observed at the sites of liver metastasis. Hepatic scintigraphy and autopsy findings revealed that the accumulation was more marked on the active cancer cells and only slight in the central necrotic tissue. Adrenal scintigraphy is valuable in the study of metastatic hormone-producing adrenal carcinoma.", "contents": "Scintigraphic detection of hepatic metastases with 131I-labeled steroid in recurrent adrenal carcinoma: case report. Abdominal scintigraphy using a new 131I-labeled steroid agent was performed on a 40-year-old women proven by surgery to have adrenocortical carcinoma. Considerable accumulations were observed at the sites of liver metastasis. Hepatic scintigraphy and autopsy findings revealed that the accumulation was more marked on the active cancer cells and only slight in the central necrotic tissue. Adrenal scintigraphy is valuable in the study of metastatic hormone-producing adrenal carcinoma.", "PMID": 966060}
{"id": "pubmed23n0032_6546", "title": "Quality assurance for 99mTc-Sn-pyrophosphate.", "content": "Because skeletal images obtained with a commercial lyophilized preparation of 99mTc-Sn-pyrophosphate were occasionally degraded by liver and spleen uptake, a method for preparing 99mTc-Sn-pyrophosphate and a system for in-house quality assurance were developed. Control of the age of the stannous chloride solution and the final pH of the 99mTc-Sn-pyrophosphate eliminated the undesirable uptake. The quality and body distribution of the in-house preparation were compared to those of the radiopharmaceutical from a commercial kit.", "contents": "Quality assurance for 99mTc-Sn-pyrophosphate. Because skeletal images obtained with a commercial lyophilized preparation of 99mTc-Sn-pyrophosphate were occasionally degraded by liver and spleen uptake, a method for preparing 99mTc-Sn-pyrophosphate and a system for in-house quality assurance were developed. Control of the age of the stannous chloride solution and the final pH of the 99mTc-Sn-pyrophosphate eliminated the undesirable uptake. The quality and body distribution of the in-house preparation were compared to those of the radiopharmaceutical from a commercial kit.", "PMID": 966061}
{"id": "pubmed23n0032_6547", "title": "A new dual-probe system for the rapid bedside assessment of left ventricular function.", "content": "A mobile dual-probe system has been developed for the rapid bedside measurement of left ventricular ejection fraction (LVEF) from the beat-to-beat count rate variations that occur during the transit of a single  radionuclide bolus through the left ventricle (LV). Cardiac output, pulmonary blood volume, and left ventricular end-diastolic volume can also be calculated. The dual-probe system incorporates a central collimated probe for monitoring activity in the LV surrounded by an annular detector collimated in such a manner as to provide simultaneous real-time monitoring of the LV background activity. The LVEFs obtained from this system correlated well with LVEFs derived from conventional single-plane contrast angiography (r=0.90) in a series of 33 patients with coronary artery disease. Positioning the dual probe over the midpoint of the LV is accomplished with standard M-mode ultrasound. Since the method is noninvasive and sensitive to volume changes in the LV, the dual probe is particularly useful for monitoring seriously ill patients as well as those with segmental wall-motion abnormalities.", "contents": "A new dual-probe system for the rapid bedside assessment of left ventricular function. A mobile dual-probe system has been developed for the rapid bedside measurement of left ventricular ejection fraction (LVEF) from the beat-to-beat count rate variations that occur during the transit of a single  radionuclide bolus through the left ventricle (LV). Cardiac output, pulmonary blood volume, and left ventricular end-diastolic volume can also be calculated. The dual-probe system incorporates a central collimated probe for monitoring activity in the LV surrounded by an annular detector collimated in such a manner as to provide simultaneous real-time monitoring of the LV background activity. The LVEFs obtained from this system correlated well with LVEFs derived from conventional single-plane contrast angiography (r=0.90) in a series of 33 patients with coronary artery disease. Positioning the dual probe over the midpoint of the LV is accomplished with standard M-mode ultrasound. Since the method is noninvasive and sensitive to volume changes in the LV, the dual probe is particularly useful for monitoring seriously ill patients as well as those with segmental wall-motion abnormalities.", "PMID": 966062}
{"id": "pubmed23n0032_6548", "title": "Direct recording on rectilinear scan images on 4x5-in. film.", "content": "Rectilinear scans are usually recorded on 14 X 17-in. x-ray film. This is costly both in terms of film and storage space. Using 4 X 5-in. film instead of the larger film will reduce this cost. An Ohio-Nuclear Model 84FD dual-probe 5-in. scanner was converted for the production of scan images directly on 4 X 5-in film (Kodak SO-79 nuclear medicine film). The smaller images may be viewed directly on a standard x-ray viewbox, or they may be projected if a larger image is desired. The image quality is excellent. This system has replaced the 14 X 17-in. film at our institution.", "contents": "Direct recording on rectilinear scan images on 4x5-in. film. Rectilinear scans are usually recorded on 14 X 17-in. x-ray film. This is costly both in terms of film and storage space. Using 4 X 5-in. film instead of the larger film will reduce this cost. An Ohio-Nuclear Model 84FD dual-probe 5-in. scanner was converted for the production of scan images directly on 4 X 5-in film (Kodak SO-79 nuclear medicine film). The smaller images may be viewed directly on a standard x-ray viewbox, or they may be projected if a larger image is desired. The image quality is excellent. This system has replaced the 14 X 17-in. film at our institution.", "PMID": 966063}
{"id": "pubmed23n0032_6549", "title": "Effect of dietary chromium on glucose tolerance and serum cholesterol in guinea pigs.", "content": "The effects of feeding three levels of dietary chromium (Cr) to non-pregnant guinea pigs, guinea pigs during pregnancy and lactation and F-1 offspring guinea pigs on weight gain, glucose tolerance, glucose peak time value and serum cholesterol concentration has been investigated. Dietary levels of Cr were: Basal Diet (B) = 0.125 ppm; B supplemented with 0.5 ppm Cr (S1 Diet) and B supplemented with 50 ppm Cr (S2 Diet). All groups had similar weight gain patterns and daily feed intake levels. Parent generation guinea pigs fed the B diet consumed less than 10 mug Cr/kg body weight/day while F-U guinea pigs consumed more than this amount. Mortality rates during pregnancy were greater in guinea pigs fed the B diet than in the Cr supplemented groups suggesting a possible protective effect by Dr. Glucose tolerance, glucose peak time values and serum cholesterol appeared to be more affected by pregnancy and generation of guinea pigs than by the level of dietary Cr. Results suggest that species differences may exist between Cr requirements of guinea pigs and rodents for avoiding glucose tolerance.", "contents": "Effect of dietary chromium on glucose tolerance and serum cholesterol in guinea pigs. The effects of feeding three levels of dietary chromium (Cr) to non-pregnant guinea pigs, guinea pigs during pregnancy and lactation and F-1 offspring guinea pigs on weight gain, glucose tolerance, glucose peak time value and serum cholesterol concentration has been investigated. Dietary levels of Cr were: Basal Diet (B) = 0.125 ppm; B supplemented with 0.5 ppm Cr (S1 Diet) and B supplemented with 50 ppm Cr (S2 Diet). All groups had similar weight gain patterns and daily feed intake levels. Parent generation guinea pigs fed the B diet consumed less than 10 mug Cr/kg body weight/day while F-U guinea pigs consumed more than this amount. Mortality rates during pregnancy were greater in guinea pigs fed the B diet than in the Cr supplemented groups suggesting a possible protective effect by Dr. Glucose tolerance, glucose peak time values and serum cholesterol appeared to be more affected by pregnancy and generation of guinea pigs than by the level of dietary Cr. Results suggest that species differences may exist between Cr requirements of guinea pigs and rodents for avoiding glucose tolerance.", "PMID": 966065}
{"id": "pubmed23n0032_6550", "title": "Postnatal patterns of brain lipids in progeny of vitamin B-6 deficient rats before and after pyridoxine supplementation.", "content": "The influence of deficient and adequate maternal intakes of pyridoxine on lipid profiles in brains of progeny at 5, 10, 15, 25 and 50 days of age was studied. The effects of supplementing deficient dams at two different times with pyridoxine on the brain development of progeny were also examined. Three groups of weanling, female rats were fed diets deficient in pyridoxine (1.2 mg pyridoxine-HC1/kg diet) and another group received a control diet (30.0 mg pyridoxine-HC1/kg diet). One deficient group and the control group were fed their diets throughout growth, gestation and lactation. Two groups of dams were fed the deficient diet through growth, gestation and until 5 or 10 days postpartum when pyridoxine was supplemented by feeding the control diet. Body and brain weights were significantly lower in 15, 25 and 50 day-old progeny of deficient dams and deficient dams supplemented at 10 days postpartum. Cerebroside content at 15 days and ganglioside content at 15 and 25 days were significantly lower in brains of pups from unsupplemented deficient dams and deficient dams supplemented at 10 days postpartum. The postnatal development of cerebroside and ganglioside levels in brain was delayed or retarded in brain of pups from unsupplemented deficient dams. Supplementation of dams fed a low level of pyridoxine (1.2 mg/kg diet) with the vitamin beginning at 5 days postpartum reversed all observed effects of the low vitamin intake on brain lipids in progeny.", "contents": "Postnatal patterns of brain lipids in progeny of vitamin B-6 deficient rats before and after pyridoxine supplementation. The influence of deficient and adequate maternal intakes of pyridoxine on lipid profiles in brains of progeny at 5, 10, 15, 25 and 50 days of age was studied. The effects of supplementing deficient dams at two different times with pyridoxine on the brain development of progeny were also examined. Three groups of weanling, female rats were fed diets deficient in pyridoxine (1.2 mg pyridoxine-HC1/kg diet) and another group received a control diet (30.0 mg pyridoxine-HC1/kg diet). One deficient group and the control group were fed their diets throughout growth, gestation and lactation. Two groups of dams were fed the deficient diet through growth, gestation and until 5 or 10 days postpartum when pyridoxine was supplemented by feeding the control diet. Body and brain weights were significantly lower in 15, 25 and 50 day-old progeny of deficient dams and deficient dams supplemented at 10 days postpartum. Cerebroside content at 15 days and ganglioside content at 15 and 25 days were significantly lower in brains of pups from unsupplemented deficient dams and deficient dams supplemented at 10 days postpartum. The postnatal development of cerebroside and ganglioside levels in brain was delayed or retarded in brain of pups from unsupplemented deficient dams. Supplementation of dams fed a low level of pyridoxine (1.2 mg/kg diet) with the vitamin beginning at 5 days postpartum reversed all observed effects of the low vitamin intake on brain lipids in progeny.", "PMID": 966066}
{"id": "pubmed23n0032_6551", "title": "Postnatal patterns of fatty acids in brain of progeny from vitamin B-6 deficient rats before and after pyridoxine supplementation.", "content": "The influence of deficient and adequate maternal intakes of pyridoxine on fatty acid profiles in brains of progeny at 5, 10, and 15 days of age was studied. The effects of two different times of initiating rehabilitation of deficient dams on the brain development of progeny at 5, 10, 15, 25, and 50 days of age were also examined. Three groups of weanling, female rats were fed diets deficient in pyridoxine (1.2 mg pyridoxine-HC1/kg diet) and a fourth group received a control diet (30.0 mg pyridoxine-HC1/kg diet) throughout growth, gestation and until 5 and 10 days postpartum. Supplementation with 30.0 mg pyridoxine-HC1/kg was begun in two deficient groups at 5 and 10 days postpartum. Fatty acids C18:2, C20:4, and C22:6 in cerebellum were significantly lower in brains of 15 day-old pups from unsupplemented deficient dams compared to values for pups of control dams. Significant reductions in the omega6 fatty acids (C18:2, C20:4, and C22:4) were evident in cerebellum of 15 day-old progeny of unsupplemented deficient dams. Fatty acids C20:1 and C24:0 were not detectable in cerebellum or cerebrum of the deficient group at 15 days but were evident in other groups. Supplementation of deficient dams with vitamin B-6 at 5 and 10 days postpartum prevented the reduction of omega6 fatty acids found in deficient progeny.", "contents": "Postnatal patterns of fatty acids in brain of progeny from vitamin B-6 deficient rats before and after pyridoxine supplementation. The influence of deficient and adequate maternal intakes of pyridoxine on fatty acid profiles in brains of progeny at 5, 10, and 15 days of age was studied. The effects of two different times of initiating rehabilitation of deficient dams on the brain development of progeny at 5, 10, 15, 25, and 50 days of age were also examined. Three groups of weanling, female rats were fed diets deficient in pyridoxine (1.2 mg pyridoxine-HC1/kg diet) and a fourth group received a control diet (30.0 mg pyridoxine-HC1/kg diet) throughout growth, gestation and until 5 and 10 days postpartum. Supplementation with 30.0 mg pyridoxine-HC1/kg was begun in two deficient groups at 5 and 10 days postpartum. Fatty acids C18:2, C20:4, and C22:6 in cerebellum were significantly lower in brains of 15 day-old pups from unsupplemented deficient dams compared to values for pups of control dams. Significant reductions in the omega6 fatty acids (C18:2, C20:4, and C22:4) were evident in cerebellum of 15 day-old progeny of unsupplemented deficient dams. Fatty acids C20:1 and C24:0 were not detectable in cerebellum or cerebrum of the deficient group at 15 days but were evident in other groups. Supplementation of deficient dams with vitamin B-6 at 5 and 10 days postpartum prevented the reduction of omega6 fatty acids found in deficient progeny.", "PMID": 966067}
{"id": "pubmed23n0032_6552", "title": "Effect of dietary copper, manganese, and zinc on nitrogen equilibrium and mineral distribution subsequent to trauma in mature rats.", "content": "Following fracture of the femur, changes in nitrogen retention and mineral tissue concentrations in response to graded levels of dietary copper, manganese, and zinc were investigated. Retained nitrogen and liver copper, manganese, zinc, nitrogen and glycogen were determined prior to trauma and at 48-hour intervals for 8 days posttrauma. The excretion of nitrogen increased, and liver copper,manganese, and zinc decreased, as did liver nitrogen and glycogen in response to fracture. Increasing dietary copper, manganese, or zinc increased liver copper, manganese, or zinc, respectively, and decreased nitrogen loss by traumatized rats. The data suggest that trauma induced a change in the concentrations of liver copper, manganese, and zinc which coincided with changes in liver nitrogen and glycogen. The results indicated that subsequent to femoral fracture, the nutritional requirement for copper, manganese, and zinc for minimal nitrogen loss were higher than the NRC requirements for the mature male rat.", "contents": "Effect of dietary copper, manganese, and zinc on nitrogen equilibrium and mineral distribution subsequent to trauma in mature rats. Following fracture of the femur, changes in nitrogen retention and mineral tissue concentrations in response to graded levels of dietary copper, manganese, and zinc were investigated. Retained nitrogen and liver copper, manganese, zinc, nitrogen and glycogen were determined prior to trauma and at 48-hour intervals for 8 days posttrauma. The excretion of nitrogen increased, and liver copper,manganese, and zinc decreased, as did liver nitrogen and glycogen in response to fracture. Increasing dietary copper, manganese, or zinc increased liver copper, manganese, or zinc, respectively, and decreased nitrogen loss by traumatized rats. The data suggest that trauma induced a change in the concentrations of liver copper, manganese, and zinc which coincided with changes in liver nitrogen and glycogen. The results indicated that subsequent to femoral fracture, the nutritional requirement for copper, manganese, and zinc for minimal nitrogen loss were higher than the NRC requirements for the mature male rat.", "PMID": 966068}
{"id": "pubmed23n0032_6553", "title": "A method to measure the adsorption of bile salts to vegetable fiber of differing water holding capacity.", "content": "Dietary vegetable fibers have physico-chemical properties which may influence their role in nutrition. A method is described which measures bile acid adsorption to fibers of differing water holding capacity. Such absorption studies are complicated by the water held in the fiber matrix which includes bile acid in solution. The entrapped solution gives some apparent adsorption but this can be removed by washing the fibers with water. This method differentiates between strong adsorption and reversible adsorption. This enables dietary vegetable fibers to be classified according to their bile acid absorption capacity which may be useful in selecting fibers for clinical trials.", "contents": "A method to measure the adsorption of bile salts to vegetable fiber of differing water holding capacity. Dietary vegetable fibers have physico-chemical properties which may influence their role in nutrition. A method is described which measures bile acid adsorption to fibers of differing water holding capacity. Such absorption studies are complicated by the water held in the fiber matrix which includes bile acid in solution. The entrapped solution gives some apparent adsorption but this can be removed by washing the fibers with water. This method differentiates between strong adsorption and reversible adsorption. This enables dietary vegetable fibers to be classified according to their bile acid absorption capacity which may be useful in selecting fibers for clinical trials.", "PMID": 966069}
{"id": "pubmed23n0032_6554", "title": "Comparative human intestinal bioavailability of vitamin B-6 from a synthetic and a natural source.", "content": "Intraluminal perfusion of human jejunum has not been previously reported to demonstrate differences in bioavailability of vitamins from a food product. In this report, a triple lumen tube with a 30 cm study segment was used to study water soluble vitamin absorption in 15 normal subjects serving as their own controls. The intestinal uptake of vitamin B-6 from organ juice (O) and from a synthetic solution (S) perfused at 7.6 ml/minute were compared. In a separate series of perfusions in six subjects, the absorption of a mixture pf pyridoxine, pyridoxal, and pyridoxamine from O and S solutions with and without glucose were compared. Mean vitamin B-6 absorption was significantly greater from S (65% uptake, transport rate 0.71 mug/cm/hour) than from 9 (30% uptake, transport rate 0.40 mug/cm/hour, p less than 0.05). The addition of glucose to S enhanced this difference. Triple lumen intestinal perfusion can be used to discriminate difference in bioavailability of water soluble vitamins from natural and synthetic sources.", "contents": "Comparative human intestinal bioavailability of vitamin B-6 from a synthetic and a natural source. Intraluminal perfusion of human jejunum has not been previously reported to demonstrate differences in bioavailability of vitamins from a food product. In this report, a triple lumen tube with a 30 cm study segment was used to study water soluble vitamin absorption in 15 normal subjects serving as their own controls. The intestinal uptake of vitamin B-6 from organ juice (O) and from a synthetic solution (S) perfused at 7.6 ml/minute were compared. In a separate series of perfusions in six subjects, the absorption of a mixture pf pyridoxine, pyridoxal, and pyridoxamine from O and S solutions with and without glucose were compared. Mean vitamin B-6 absorption was significantly greater from S (65% uptake, transport rate 0.71 mug/cm/hour) than from 9 (30% uptake, transport rate 0.40 mug/cm/hour, p less than 0.05). The addition of glucose to S enhanced this difference. Triple lumen intestinal perfusion can be used to discriminate difference in bioavailability of water soluble vitamins from natural and synthetic sources.", "PMID": 966070}
{"id": "pubmed23n0032_6555", "title": "Influence of diet on glucose tolerance, on the rate of glucose utilization and on gluconeogenic enzyme activities in the dog.", "content": "Four diets containing 0 to 62% of energy from carbohydrate and 24% to 48% of energy from protein were fed to young dogs. Two of the diets (diets LPLC and HPLC) were carbohydrate-free and two of the diets (diets HPHC and HPLC) contained 46% to 48% protein. The influence of these diets on several parameters of glucose metabolism was ascertained. Following an intravenous glucose load, plasma glucose levels were higher in dogs fed carbohydrate-free diets (diets LPLC and HPLC) than observed in dogs fed carbohydrate-containing diets (diet LPHC and HPHC). Consumption of high-protein diets (diets HPHC and HPLC) also impaired glucose tolerance. Estimates of glucose utilization were obtained. Dogs fed carbohydrate-containing diets exhibited a higher rate of glucose utilization than did dogs fed the carbohydrate-free diets. Fasting the dogs for 48 hours reduced the glucose replacement rate in dogs fed the carbohydrate-containing diets but did not influence the rate of glucose utilization in dogs fed the carbohydrate-free diets. The activities of phosphoenolpyruvate carboxykinase (PEPCK) and of pyruvate carboxylase (PYCAR) in livers and kidneys of these dogs were influenced by the diets fed. Mitochondrial PEPCK and PYCAR activities in both liver and kidney were increased in dogs fed the carbohydrate-free diets. Consumption of the high-protein diets actually decreased the activities of PEPCK in liver and kidney mitochondria.", "contents": "Influence of diet on glucose tolerance, on the rate of glucose utilization and on gluconeogenic enzyme activities in the dog. Four diets containing 0 to 62% of energy from carbohydrate and 24% to 48% of energy from protein were fed to young dogs. Two of the diets (diets LPLC and HPLC) were carbohydrate-free and two of the diets (diets HPHC and HPLC) contained 46% to 48% protein. The influence of these diets on several parameters of glucose metabolism was ascertained. Following an intravenous glucose load, plasma glucose levels were higher in dogs fed carbohydrate-free diets (diets LPLC and HPLC) than observed in dogs fed carbohydrate-containing diets (diet LPHC and HPHC). Consumption of high-protein diets (diets HPHC and HPLC) also impaired glucose tolerance. Estimates of glucose utilization were obtained. Dogs fed carbohydrate-containing diets exhibited a higher rate of glucose utilization than did dogs fed the carbohydrate-free diets. Fasting the dogs for 48 hours reduced the glucose replacement rate in dogs fed the carbohydrate-containing diets but did not influence the rate of glucose utilization in dogs fed the carbohydrate-free diets. The activities of phosphoenolpyruvate carboxykinase (PEPCK) and of pyruvate carboxylase (PYCAR) in livers and kidneys of these dogs were influenced by the diets fed. Mitochondrial PEPCK and PYCAR activities in both liver and kidney were increased in dogs fed the carbohydrate-free diets. Consumption of the high-protein diets actually decreased the activities of PEPCK in liver and kidney mitochondria.", "PMID": 966071}
{"id": "pubmed23n0032_6556", "title": "Effect of stable strontium on the tissue alkaline and acid phosphatase activities of rat: feeding studies.", "content": "The effect of feeding stable strontium (Sr) on the tissue alkaline and acid phosphatase activities was studied in young rats. These activities were reduced in liver and small intestine by 10% to 15% at 2 weeks, 20% to 30% at 4 weeks and in kidney by 20% at 6 weeks only in rats fed 2% Sr diet; bone alkaliine phosphatase activity was, however, increased by 80% to 100% (2-6 weeks) in these rats. Gross lesions like paralysis, hemorrhage, rickets and high mortality were observed after 4 to 6 weeks. Although no such lesions were seen, appreciable changes in enzyme activities as mentioned above were discernible in rats fed 1% Sr diet for 6 weeks. Feeding of a 0.5% Sr diet for a period up to 6 weeks had no deleterious effect. Recovery following consumption of a normal diet for 2 weeks was almost complete in liver and small intestine but not in kidney. The elevated tissue Sr levels do not explain the pronounced losses seen in this investigation as compared to those in the earlier in vitro experiments. This study depicts the possible damage due to prolonged therapeutic use of large amounts of stable Sr for the removal of radiostrontium.", "contents": "Effect of stable strontium on the tissue alkaline and acid phosphatase activities of rat: feeding studies. The effect of feeding stable strontium (Sr) on the tissue alkaline and acid phosphatase activities was studied in young rats. These activities were reduced in liver and small intestine by 10% to 15% at 2 weeks, 20% to 30% at 4 weeks and in kidney by 20% at 6 weeks only in rats fed 2% Sr diet; bone alkaliine phosphatase activity was, however, increased by 80% to 100% (2-6 weeks) in these rats. Gross lesions like paralysis, hemorrhage, rickets and high mortality were observed after 4 to 6 weeks. Although no such lesions were seen, appreciable changes in enzyme activities as mentioned above were discernible in rats fed 1% Sr diet for 6 weeks. Feeding of a 0.5% Sr diet for a period up to 6 weeks had no deleterious effect. Recovery following consumption of a normal diet for 2 weeks was almost complete in liver and small intestine but not in kidney. The elevated tissue Sr levels do not explain the pronounced losses seen in this investigation as compared to those in the earlier in vitro experiments. This study depicts the possible damage due to prolonged therapeutic use of large amounts of stable Sr for the removal of radiostrontium.", "PMID": 966072}
{"id": "pubmed23n0032_6557", "title": "Effect of dietary protein depletion on the galactose elimination capacity in intact rats.", "content": "The galactose elimination capacity (GEC) of rats fed low protein liquid diets and libitum with 5% or 0.5% of the energy as protein was compared with that of a control group receiving a similar diet with 25% of the energy as protein. The daily dietary energy intake was almost identical in the three groups. The rats fed the 5% protein diet showed slightly higher GEC than the control group. In the rats receiving the 0.5% protein diet, GEC was significantly decreased to approximately half of the control values and the hepatic protein content was reduced to the same extent. Body weight and liver weight showed reductions to approximately 75% of control values. Triglyceride content in the livers was increased five times in the protein depleted rats. The results indicate that GEC in rats deprived of dietary protein is determined by the amount of hepatic protein.", "contents": "Effect of dietary protein depletion on the galactose elimination capacity in intact rats. The galactose elimination capacity (GEC) of rats fed low protein liquid diets and libitum with 5% or 0.5% of the energy as protein was compared with that of a control group receiving a similar diet with 25% of the energy as protein. The daily dietary energy intake was almost identical in the three groups. The rats fed the 5% protein diet showed slightly higher GEC than the control group. In the rats receiving the 0.5% protein diet, GEC was significantly decreased to approximately half of the control values and the hepatic protein content was reduced to the same extent. Body weight and liver weight showed reductions to approximately 75% of control values. Triglyceride content in the livers was increased five times in the protein depleted rats. The results indicate that GEC in rats deprived of dietary protein is determined by the amount of hepatic protein.", "PMID": 966073}
{"id": "pubmed23n0032_6558", "title": "Evidence that vitamin A is not required for the biosynthesis of ovalbumin in chicks.", "content": "Four-day-old pullets fed a vitamin A-deficient diet were stimulated daily with 1 mg 17beta-estradiol-3-benzoate/day for 6 to 19 days. The onset of vitamin A deficiency had no effect on oviduct growth in these chicks; even though vitamin A-deficient chicks showed a severe decline in growth rate while controls (fed the same diet supplemented with retinyl palmitate) continued to grow, estrogen stimulated resulted in similar oviduct size. Ovalbumin concentrations of estrogen-stimulated chicks were determined by immunoprecipitation of the soluble protein supernatant fraction of oviduct. The concentration of ovalbumin in oviducts of chicks fed a vitamin A-supplemented diet was similar in the concentration in oviducts of chicks fed a vitamin A-deficient diet. The incorporation of [3H]glucosamine and 14C-amino acids into immunoprecipitable ovalbumin, following the in vitro incubation of minced oviduct, indicated that ovalbumin synthesis was not affected by vitamin A deficiency. The specific activity of incorporated [3H]glucosamine, the 14C-amino acid incorporation into ovalbumin, the relative rate of ovalbumin synthesis, and the relative effiency of [3H]glucosamine incorporation into ovalbumin were each similar between the two diet groups. The relative efficiency of [3H]glucosamine incorporation into sodium dodecyl sulfate and dithiothreitol extractable membranous proteins of oviduct was not affect by vitamin A deficiency.", "contents": "Evidence that vitamin A is not required for the biosynthesis of ovalbumin in chicks. Four-day-old pullets fed a vitamin A-deficient diet were stimulated daily with 1 mg 17beta-estradiol-3-benzoate/day for 6 to 19 days. The onset of vitamin A deficiency had no effect on oviduct growth in these chicks; even though vitamin A-deficient chicks showed a severe decline in growth rate while controls (fed the same diet supplemented with retinyl palmitate) continued to grow, estrogen stimulated resulted in similar oviduct size. Ovalbumin concentrations of estrogen-stimulated chicks were determined by immunoprecipitation of the soluble protein supernatant fraction of oviduct. The concentration of ovalbumin in oviducts of chicks fed a vitamin A-supplemented diet was similar in the concentration in oviducts of chicks fed a vitamin A-deficient diet. The incorporation of [3H]glucosamine and 14C-amino acids into immunoprecipitable ovalbumin, following the in vitro incubation of minced oviduct, indicated that ovalbumin synthesis was not affected by vitamin A deficiency. The specific activity of incorporated [3H]glucosamine, the 14C-amino acid incorporation into ovalbumin, the relative rate of ovalbumin synthesis, and the relative effiency of [3H]glucosamine incorporation into ovalbumin were each similar between the two diet groups. The relative efficiency of [3H]glucosamine incorporation into sodium dodecyl sulfate and dithiothreitol extractable membranous proteins of oviduct was not affect by vitamin A deficiency.", "PMID": 966074}
{"id": "pubmed23n0032_6559", "title": "The effect of an acute dose of biotin at a post-implantation stage and its relation with female sex steriods in the rat.", "content": "An acute dose (10 mg/100 g body weight) of biotin at the post-implantation stage (day 14 and 15) inhibited the fetal and placental growth, and in few rats it also caused resorption of fetuses and placentae. The maintenance of pregnancy with normal fetal and placental growth was effected with estrogen therapy, but progesterone failed to correct the biotin-induced effect. The uterine and placental glycogen, RNA and protein levels, as well as, glucose-6-phosphate dehydrogenase activity in the ovary, liver and uterus showed a reduction following biotin treatment. Estrogen therapy under such conditions corrected these adverse effects of biotin overdose, while progesterone had no significant effects. The study suggests that the acute dose of biotin at an advance stage of pregnancy may cause adverse effects on the physiological regulation of gestation, possibly by creating deficiency of estrogen and gestagen. The possible role of estrogen in the fetal and placental growth and regulation of gestagen secretion is discussed.", "contents": "The effect of an acute dose of biotin at a post-implantation stage and its relation with female sex steriods in the rat. An acute dose (10 mg/100 g body weight) of biotin at the post-implantation stage (day 14 and 15) inhibited the fetal and placental growth, and in few rats it also caused resorption of fetuses and placentae. The maintenance of pregnancy with normal fetal and placental growth was effected with estrogen therapy, but progesterone failed to correct the biotin-induced effect. The uterine and placental glycogen, RNA and protein levels, as well as, glucose-6-phosphate dehydrogenase activity in the ovary, liver and uterus showed a reduction following biotin treatment. Estrogen therapy under such conditions corrected these adverse effects of biotin overdose, while progesterone had no significant effects. The study suggests that the acute dose of biotin at an advance stage of pregnancy may cause adverse effects on the physiological regulation of gestation, possibly by creating deficiency of estrogen and gestagen. The possible role of estrogen in the fetal and placental growth and regulation of gestagen secretion is discussed.", "PMID": 966075}
{"id": "pubmed23n0032_6560", "title": "Fundamental studies on physiological and pharmacological actions of L-ascorbate 2-sulfate. I. On the hypolipidemic effects.", "content": "The effects of L-ascorbate 2-sulfate (AAS) on the lipid metabolism were examined in Triton-induced hyperlipemic mice, hypercholesterolemic and normal rats, the following results being obtained. 1) In Triton-induced hyperlipemic mice, AAS (300 mg/kg) significantly decreased the serum cholesterol level, while L-ascorbate (AA, 175 mg/kg) was found ineffective. 2)In hypercholesterolemic rats fed 0.5% cholesterol diet, the consecutive administration of AAS decreased the level of serum cholesterol and liver triacylglycerols. AA only slightly affected these levels. However, both AAS and AA prevented the unordinal increase in the liver weight caused by cholesterol feeding. 3)In normal rats, the administration of AAS over a 4-week period decreased the levels of serum cholesterol and liver triacylglycerols.", "contents": "Fundamental studies on physiological and pharmacological actions of L-ascorbate 2-sulfate. I. On the hypolipidemic effects. The effects of L-ascorbate 2-sulfate (AAS) on the lipid metabolism were examined in Triton-induced hyperlipemic mice, hypercholesterolemic and normal rats, the following results being obtained. 1) In Triton-induced hyperlipemic mice, AAS (300 mg/kg) significantly decreased the serum cholesterol level, while L-ascorbate (AA, 175 mg/kg) was found ineffective. 2)In hypercholesterolemic rats fed 0.5% cholesterol diet, the consecutive administration of AAS decreased the level of serum cholesterol and liver triacylglycerols. AA only slightly affected these levels. However, both AAS and AA prevented the unordinal increase in the liver weight caused by cholesterol feeding. 3)In normal rats, the administration of AAS over a 4-week period decreased the levels of serum cholesterol and liver triacylglycerols.", "PMID": 966076}
{"id": "pubmed23n0032_6561", "title": "Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle.", "content": "Standardized and reliable methods have been devised to assay preparations of the glutamic oxaloacetic transaminase from human erythrocytes by the CAS principle, i.e., the unsaturation and saturation of the coenzyme (pyridoxal 5'-phosphate) of a Coenzyme-Apoenzyme System. This dual enzymic assay is effective to detect and measure human deficiencies of vitamin B6. A preferred procedure has been developed, particularly for individuals and for limited groups, but is also applicable to large groups. A simplified procedure, which emphasizes the statistical significance of difference between large groups was also developed. Lyophilized erythrocyte hemolysates appear stable for up to 7 days for simplified transportation.", "contents": "Improved and effective assays of the glutamic oxaloacetic transaminase by the coenzyme-apoenzyme system (CAS) principle. Standardized and reliable methods have been devised to assay preparations of the glutamic oxaloacetic transaminase from human erythrocytes by the CAS principle, i.e., the unsaturation and saturation of the coenzyme (pyridoxal 5'-phosphate) of a Coenzyme-Apoenzyme System. This dual enzymic assay is effective to detect and measure human deficiencies of vitamin B6. A preferred procedure has been developed, particularly for individuals and for limited groups, but is also applicable to large groups. A simplified procedure, which emphasizes the statistical significance of difference between large groups was also developed. Lyophilized erythrocyte hemolysates appear stable for up to 7 days for simplified transportation.", "PMID": 966077}
{"id": "pubmed23n0032_6562", "title": "Fatty acid composition of adipose tissue and liver fats of the rats fed yeast grown on n-alkanes.", "content": "n-Alkane assimilating yeast, Candida tropicalis YO-148, was grown on an n-alkane-containing medium. A synthetic diet containing 6.8% of dried yeast was fed to rats. The fatty acid composition of adipose tissue and liver fats was determined after a two week feeding period. The percentage of odd-numbered acids increased in the animals fed the yeast diet. Furthermore, it was shown that heptadecenoic acid, the major odd-numbered acid in yeast, was accumulated in neutral lipid fraction of adipose tissue fat. Fatty acid composition of protein isolate prepared from yeast cells had a profile similar to that of the original cells.", "contents": "Fatty acid composition of adipose tissue and liver fats of the rats fed yeast grown on n-alkanes. n-Alkane assimilating yeast, Candida tropicalis YO-148, was grown on an n-alkane-containing medium. A synthetic diet containing 6.8% of dried yeast was fed to rats. The fatty acid composition of adipose tissue and liver fats was determined after a two week feeding period. The percentage of odd-numbered acids increased in the animals fed the yeast diet. Furthermore, it was shown that heptadecenoic acid, the major odd-numbered acid in yeast, was accumulated in neutral lipid fraction of adipose tissue fat. Fatty acid composition of protein isolate prepared from yeast cells had a profile similar to that of the original cells.", "PMID": 966078}
{"id": "pubmed23n0032_6563", "title": "Effect of occupational and nonoccupational factors on the respiratory system of vinyl chloride and other workers.", "content": "There are suggestions in the literature that vinyl chloride (VC) acts as a lung irritant. Respiratory questionnaires and lung function tests were administered to 174 chemical (VC) workers, 81 polyvinyl chloride (PVC) workers, 72 former VC workers, and 136 rubber workers, and 68 maintenance workers with exposure to VC, PVC, and rubber. Except for small airways obstruction associated with rubber, increased respiratory symptoms and decreased pulmonary function were not associated with working in chemicals, plastics, or rubber. Some increases in baseline pulmonary function were associated with VC exposure. Acute reductions in pulmonary function were observed in smokers working in chemicals, plastics, and rubber. Heavier cigarette smokers over 40 years of age had the most adversely affected respiratory system. Work was not associated with chronic respiratory effects, but all exposure groups experienced some acute respiratory insult.", "contents": "Effect of occupational and nonoccupational factors on the respiratory system of vinyl chloride and other workers. There are suggestions in the literature that vinyl chloride (VC) acts as a lung irritant. Respiratory questionnaires and lung function tests were administered to 174 chemical (VC) workers, 81 polyvinyl chloride (PVC) workers, 72 former VC workers, and 136 rubber workers, and 68 maintenance workers with exposure to VC, PVC, and rubber. Except for small airways obstruction associated with rubber, increased respiratory symptoms and decreased pulmonary function were not associated with working in chemicals, plastics, or rubber. Some increases in baseline pulmonary function were associated with VC exposure. Acute reductions in pulmonary function were observed in smokers working in chemicals, plastics, and rubber. Heavier cigarette smokers over 40 years of age had the most adversely affected respiratory system. Work was not associated with chronic respiratory effects, but all exposure groups experienced some acute respiratory insult.", "PMID": 966081}
{"id": "pubmed23n0032_6564", "title": "How is the acceptability of risks to the health of the workers to be determined?", "content": "The determination of risk at work is a shared responsibility of the employer and the employee, but objective evaluation of determining risk may be outweighed by economic considerations. Who and in what instances a person shall work is further modified by legal, social, and cultural restraints. The physician, as the agent of the employer, is often a key factor in deciding the real risk inherent in working in a specific environment, in informing management of the risk, and in determining which worker will accept the risk. The conscientiousness, competence, and ethical character of the physician are challenged by these responsibilities and circumstances, and a code of behavior is outlined for professional conduct of the occupational physician.", "contents": "How is the acceptability of risks to the health of the workers to be determined? The determination of risk at work is a shared responsibility of the employer and the employee, but objective evaluation of determining risk may be outweighed by economic considerations. Who and in what instances a person shall work is further modified by legal, social, and cultural restraints. The physician, as the agent of the employer, is often a key factor in deciding the real risk inherent in working in a specific environment, in informing management of the risk, and in determining which worker will accept the risk. The conscientiousness, competence, and ethical character of the physician are challenged by these responsibilities and circumstances, and a code of behavior is outlined for professional conduct of the occupational physician.", "PMID": 966083}
{"id": "pubmed23n0032_6565", "title": "Hepatitis among plasma fractionation workers. An industry-wide study.", "content": "An industry-wide survey of plasm fractionation facilities in the United States was conducted during 1973-74. Hepatitis was reported among the workers with varying degrees of plasma contact at all plant sites. For each of fourteen facilities in this investigation, field inspections disclosed: (1) numerous overt instances of employee-product contact: (2) inconsistent methodologic approaches at virtually all stages of the plasma fractionation process; (3) at low level of management and employee awareness regarding the potential bio-hazard identified; and (4) disparate examples of hepatitis surveillance and prophylaxis. Our data suggests that there is a high risk of hepatitis among plasma fractionation workers and, as such, much more attention needs to be focused on the reduction of health hazards within this industry.", "contents": "Hepatitis among plasma fractionation workers. An industry-wide study. An industry-wide survey of plasm fractionation facilities in the United States was conducted during 1973-74. Hepatitis was reported among the workers with varying degrees of plasma contact at all plant sites. For each of fourteen facilities in this investigation, field inspections disclosed: (1) numerous overt instances of employee-product contact: (2) inconsistent methodologic approaches at virtually all stages of the plasma fractionation process; (3) at low level of management and employee awareness regarding the potential bio-hazard identified; and (4) disparate examples of hepatitis surveillance and prophylaxis. Our data suggests that there is a high risk of hepatitis among plasma fractionation workers and, as such, much more attention needs to be focused on the reduction of health hazards within this industry.", "PMID": 966085}
{"id": "pubmed23n0032_6566", "title": "Chronic respiratory symptoms and job type within the rubber industry.", "content": "Associations between the presence of chronic respiratory symptoms and a history of working in certain production job categories within the rubber industry are presented. Environmental explanations for these associations are discussed. Particular attention is also paid to the methodologic diffculties in analyzing cross-sectional health data in relation to the mobile and environmentally varied work-histories of individual workers within the rubber industry.", "contents": "Chronic respiratory symptoms and job type within the rubber industry. Associations between the presence of chronic respiratory symptoms and a history of working in certain production job categories within the rubber industry are presented. Environmental explanations for these associations are discussed. Particular attention is also paid to the methodologic diffculties in analyzing cross-sectional health data in relation to the mobile and environmentally varied work-histories of individual workers within the rubber industry.", "PMID": 966093}
{"id": "pubmed23n0032_6567", "title": "Safe and unsafe behavior and its modification.", "content": "After discussing some of the limitations of existing behavioral safety research, the authors propose a new approach to the problem. The approach is based on systematically controlling the psychological environment by precisely defining and rewarding safe behabiors and extinguishing unsafe behaviors. This approach to changing behavior is broadly designated the experimental analysis of behavior, and comprises two separate components, applied behavior analysis and applied behavior management. Application of the principles to the problem of increasing the frequency of safe behaviors and decreasing the frequency of unsafe behaviors is illustrated with two examples, one real, one hypothetical. A conclusion is drawn that researchers pursuing behavioral approaches to accident reduction should utilize the methods and technology of applied behavior analysis and management.", "contents": "Safe and unsafe behavior and its modification. After discussing some of the limitations of existing behavioral safety research, the authors propose a new approach to the problem. The approach is based on systematically controlling the psychological environment by precisely defining and rewarding safe behabiors and extinguishing unsafe behaviors. This approach to changing behavior is broadly designated the experimental analysis of behavior, and comprises two separate components, applied behavior analysis and applied behavior management. Application of the principles to the problem of increasing the frequency of safe behaviors and decreasing the frequency of unsafe behaviors is illustrated with two examples, one real, one hypothetical. A conclusion is drawn that researchers pursuing behavioral approaches to accident reduction should utilize the methods and technology of applied behavior analysis and management.", "PMID": 966094}
{"id": "pubmed23n0032_6568", "title": "Ocular optics and evolution.", "content": "The application of sampling theory to image formation in the human eye is related to the population density of photoreceptors. A close numerical agreement is revealed which suggests that an image-forming device is of cardinal importance in ocular evolution.", "contents": "Ocular optics and evolution. The application of sampling theory to image formation in the human eye is related to the population density of photoreceptors. A close numerical agreement is revealed which suggests that an image-forming device is of cardinal importance in ocular evolution.", "PMID": 966098}
{"id": "pubmed23n0032_6569", "title": "Changes of the postoperative minimal oxygen consumption of the newborn.", "content": "Minimal oxygen consumption and carbon dioxide production were studied in newborns without operation and with minor or major operation by means of open indirect calorimetry. (1) A postnatal increase in the mVO2 was observed in most of the full-term newborns without operation or with minor operation. (2) A postoperative increase in the mVO2, as observed in the adult, was not found in all newborns with a major operation. (3) This finding was particularly obvious in the newborn with a major abdominal operation and with a long pre- and postoperative periods of starvation. (4) The most important factor determining the postoperative decrease in the mVO2 is not the intensity of operative stress but the amount of caloric intake.", "contents": "Changes of the postoperative minimal oxygen consumption of the newborn. Minimal oxygen consumption and carbon dioxide production were studied in newborns without operation and with minor or major operation by means of open indirect calorimetry. (1) A postnatal increase in the mVO2 was observed in most of the full-term newborns without operation or with minor operation. (2) A postoperative increase in the mVO2, as observed in the adult, was not found in all newborns with a major operation. (3) This finding was particularly obvious in the newborn with a major abdominal operation and with a long pre- and postoperative periods of starvation. (4) The most important factor determining the postoperative decrease in the mVO2 is not the intensity of operative stress but the amount of caloric intake.", "PMID": 966114}
{"id": "pubmed23n0032_6570", "title": "Transumbilical aortography in newborn infants for noncardiac diagnosis.", "content": "Transumbilical aortography and selective arteriography were performed in three newborn infants for the evaluation of massive hepatomegaly, large sacrococcygeal teratoma and extensive tumor of the thigh. The study was valuable in all cases and resulted in no complication.", "contents": "Transumbilical aortography in newborn infants for noncardiac diagnosis. Transumbilical aortography and selective arteriography were performed in three newborn infants for the evaluation of massive hepatomegaly, large sacrococcygeal teratoma and extensive tumor of the thigh. The study was valuable in all cases and resulted in no complication.", "PMID": 966115}
{"id": "pubmed23n0032_6571", "title": "Cutaneous loop versus Roux-en-Y ureterostomy: a comparison of two methods for diversion of urine.", "content": "Loop and Roux-en-Y ureterostomies are compared. Both methods have a place if diversion is necessary in cases of severe hydronephrosis and decompensated ureters. Although the loop ureterostomy has advantages, it can lead to problems, i.e., the nonfunctioning and the contracted bladder. This serious complication is not seen after Roux-en-Y ureterostomy, because the bladder continues to function. The localization of the new pyeloureteral junction is discussed as the formation of kinks in this region must be avoided.", "contents": "Cutaneous loop versus Roux-en-Y ureterostomy: a comparison of two methods for diversion of urine. Loop and Roux-en-Y ureterostomies are compared. Both methods have a place if diversion is necessary in cases of severe hydronephrosis and decompensated ureters. Although the loop ureterostomy has advantages, it can lead to problems, i.e., the nonfunctioning and the contracted bladder. This serious complication is not seen after Roux-en-Y ureterostomy, because the bladder continues to function. The localization of the new pyeloureteral junction is discussed as the formation of kinks in this region must be avoided.", "PMID": 966116}
{"id": "pubmed23n0032_6572", "title": "Complicated giant hemangioma: excision using cardiopulmonary bypass and deep hypothermia.", "content": "A child with a giant hemangioma of the left scalp, neck, and upper chest had severe complications including congestive cardiac failure. Surgical excision using cardiac bypass, deep hypothermia, and circulatory arrest was successfully carried out.", "contents": "Complicated giant hemangioma: excision using cardiopulmonary bypass and deep hypothermia. A child with a giant hemangioma of the left scalp, neck, and upper chest had severe complications including congestive cardiac failure. Surgical excision using cardiac bypass, deep hypothermia, and circulatory arrest was successfully carried out.", "PMID": 966117}
{"id": "pubmed23n0032_6573", "title": "A new approach to the diagnosis of testicular torsion.", "content": "The acutely painful, enlarged scrotum has necessitated urgent exploration of the scrotum so as not to miss testicular torsion. This has led to unnecessary operations. The urgency with which the diagnosis is made is particularly important in infants and children for in these patients the signs and symptoms of testicular torsion may be minimal. Since its introduction in 1973, scrotal scanning has allowed a precise evaluation of the testicular perfusion and thus refined the indications for operation. Using scrotal scanning in a series of 18 patients with possible testicular torsion a correct assessment of testicular perfusion was made in every patient. In only two patients did the interpretation differ from the ultimate clinical impression. In one, the torsion had relieved itself before the scan was performed and at operation the testis had adequate perfusion. In the second, clinically suspected epididymitis was not confirmed. Because of the ease and rapidity with which the test may be done in children more testes should be salvaged with fewer negative explorations.", "contents": "A new approach to the diagnosis of testicular torsion. The acutely painful, enlarged scrotum has necessitated urgent exploration of the scrotum so as not to miss testicular torsion. This has led to unnecessary operations. The urgency with which the diagnosis is made is particularly important in infants and children for in these patients the signs and symptoms of testicular torsion may be minimal. Since its introduction in 1973, scrotal scanning has allowed a precise evaluation of the testicular perfusion and thus refined the indications for operation. Using scrotal scanning in a series of 18 patients with possible testicular torsion a correct assessment of testicular perfusion was made in every patient. In only two patients did the interpretation differ from the ultimate clinical impression. In one, the torsion had relieved itself before the scan was performed and at operation the testis had adequate perfusion. In the second, clinically suspected epididymitis was not confirmed. Because of the ease and rapidity with which the test may be done in children more testes should be salvaged with fewer negative explorations.", "PMID": 966118}
{"id": "pubmed23n0032_6574", "title": "Infarction of the common bile duct in an infant after surfical exploration for jaundice: a possible cause of extrahepatic biliary atresia.", "content": "An infant explored for obstructive jaundice died 6 days postoperatively and was found to have extensive infarction of the common duct. This would have gone undetected had the patient survived and without careful sampling at autopsy. The incidence of this unsuspected complication is unknown. Surgical infarction may be one cause of fibrosis and secondary atresia of the extra-hepatic bile ducts.", "contents": "Infarction of the common bile duct in an infant after surfical exploration for jaundice: a possible cause of extrahepatic biliary atresia. An infant explored for obstructive jaundice died 6 days postoperatively and was found to have extensive infarction of the common duct. This would have gone undetected had the patient survived and without careful sampling at autopsy. The incidence of this unsuspected complication is unknown. Surgical infarction may be one cause of fibrosis and secondary atresia of the extra-hepatic bile ducts.", "PMID": 966119}
{"id": "pubmed23n0032_6575", "title": "The internal anal sphincter in translevator (low) anal anomalies.", "content": "Internal sphincteric function was studied manometrically in 19 children with translevator anal anomalies. The normal internal sphincter inhibitory response to rectal distension was present in ten pateints, attenuated in six, and absent in three. Our findings suggest that a functional internal sphincter is usually present in translevator anal anomalies.", "contents": "The internal anal sphincter in translevator (low) anal anomalies. Internal sphincteric function was studied manometrically in 19 children with translevator anal anomalies. The normal internal sphincter inhibitory response to rectal distension was present in ten pateints, attenuated in six, and absent in three. Our findings suggest that a functional internal sphincter is usually present in translevator anal anomalies.", "PMID": 966120}
{"id": "pubmed23n0032_6576", "title": "The painless intussusception.", "content": "There were 422 cases of intussusception at the Hospital for Sick Children, Toronto, over a 12-yr period between 1959 and 1970. The histories of all patients who had no pain recorded were evaluated and the duration of signs and symptoms and the treatment required assessed. When compared with those who had pain, it was noted that the children with painless intussusception presented later, hydrostatic reduction was less likely to be effective, and operative reduction and resection was required more often.", "contents": "The painless intussusception. There were 422 cases of intussusception at the Hospital for Sick Children, Toronto, over a 12-yr period between 1959 and 1970. The histories of all patients who had no pain recorded were evaluated and the duration of signs and symptoms and the treatment required assessed. When compared with those who had pain, it was noted that the children with painless intussusception presented later, hydrostatic reduction was less likely to be effective, and operative reduction and resection was required more often.", "PMID": 966121}
{"id": "pubmed23n0032_6577", "title": "Pseudolymphoma presenting as a cecal tumor.", "content": "A 7-yr-old with abdominal pain and melena had a barium enema which revealed a cecal lesion that proved to be a pseudolymphoma of the cecum and appendix--a site not previously reported.", "contents": "Pseudolymphoma presenting as a cecal tumor. A 7-yr-old with abdominal pain and melena had a barium enema which revealed a cecal lesion that proved to be a pseudolymphoma of the cecum and appendix--a site not previously reported.", "PMID": 966122}
{"id": "pubmed23n0032_6578", "title": "Urine extravasation secondary to upper urinary tract obstruction.", "content": "To the best of our knowledge, this is the first reported case of renal salvage in a neonate with presumed spontaneous perirenal leakage of urine associated with ureteropelvic junction obstruction. Many instances of such extravasation in this age group have been described with lower urinary tract obstructions. As in the latter cases, the occurrence of extravasation secondary to upper tract obstruction need not preclude primary reconstruction.", "contents": "Urine extravasation secondary to upper urinary tract obstruction. To the best of our knowledge, this is the first reported case of renal salvage in a neonate with presumed spontaneous perirenal leakage of urine associated with ureteropelvic junction obstruction. Many instances of such extravasation in this age group have been described with lower urinary tract obstructions. As in the latter cases, the occurrence of extravasation secondary to upper tract obstruction need not preclude primary reconstruction.", "PMID": 966123}
{"id": "pubmed23n0032_6579", "title": "Ureteral triplication.", "content": "A case of ureteral triplication is presented. There was reflux of the middle ureter, which did not end in the kidney, but rather ended blindly. There was atrophic pyelonephritis of the upper pole because of an obstructed ureter, and a normal lower caleceal system and ureter. There were three ureters but only two originated in the kidney and two entered the bladder, with only one ureter originating from the kidney entering the bladder normally. The refluxing ureter and the obstructed upper pole and ureter were removed.", "contents": "Ureteral triplication. A case of ureteral triplication is presented. There was reflux of the middle ureter, which did not end in the kidney, but rather ended blindly. There was atrophic pyelonephritis of the upper pole because of an obstructed ureter, and a normal lower caleceal system and ureter. There were three ureters but only two originated in the kidney and two entered the bladder, with only one ureter originating from the kidney entering the bladder normally. The refluxing ureter and the obstructed upper pole and ureter were removed.", "PMID": 966124}
{"id": "pubmed23n0032_6580", "title": "Angiomatous malformation of the testis in an infant.", "content": "A 2-mo-old infant with a benign vascular lesion of the right testis and epididymus was presented. The lesion was diagnosed by incisional biopsy avoiding orchiectomy. One year later, the lesion has not increased in size. A careful follow-up of patients with these rare lesions is required to increase our knowledge about the pathogenesis.", "contents": "Angiomatous malformation of the testis in an infant. A 2-mo-old infant with a benign vascular lesion of the right testis and epididymus was presented. The lesion was diagnosed by incisional biopsy avoiding orchiectomy. One year later, the lesion has not increased in size. A careful follow-up of patients with these rare lesions is required to increase our knowledge about the pathogenesis.", "PMID": 966126}
{"id": "pubmed23n0032_6581", "title": "The use of dextrostix and dextrostix reflectance meters in the diagnosis of neonatal hypoglycemia.", "content": "Dextrostix is now widely used as a method of screening for hypoglycemia of the newborn. There has always been some anxiety about its accuracy for estimating very low blood sugars; this is important, since symptomatic hypoglycemia of the newborn does not usually occur until the blood glucose falls below 20 mg/dl. In 1970 a reflectance meter was introduced which would measure the colour of the strip electronically. The aim of this study was to assess the accuracy of the meter and its reliability in estimating hypoglycemia. The study was continued to investigate a new model of the reflectance meter which superseded the original one. In the pilot study using the original model, blood samples were taken from 46 babies. Readings of the Dextrostix were made by two independent observers and compared with a reading taken on the reflectance meter. These estimations were later compared with blood glucose measured by the glucose oxidase method. the meter showed a marked tendency to overestimate the blood glucose: 44 out of 46 samples were overestimated (Fig. 1, 2). In the second series 180 cord blood samples were collected. Because the intention was to study very low levels of blood glucose, the samples were allowed to stand at room temperature for several hours to allow glycolysis to occur. Again, readings were taken by two independent observers and compared with the readings taken on the new Dextrostix-Eyetone meter. The blood glucose was measured on each of the samples. There was a strong correlation (r = 0.8877, p less than 0.00005) between the blood glucose values and the readings taken from the meter, with no tendency towards overestimation (Fig. 3). A similar correlation (r = 0.8533, p less than 0.00005) was seen for the observers' readings and the chemical method, although there was a tendency to underestimate blood glucose (Fig. 4). When the meter gave an estimate of more than 20 ml/dl, in no case was the actual blood glucose in the profoundly hypoglycemic group of less than 10 mg/dl. When estimated by eye there was one case in which the blood glucose was only 7 mg/dl but the observer had estimated the result of 30 mg/dl. In order to avoid any possibility that a blood glucose was less than 20 mg/dl, it is necessary to take action on any estimate below 40 mg/dl on a Dextrostix. Despite this, Dextrostix remains a very useful method of screening for neonatal hypoglycemia, whether assessed by eye or with the new Dextrostix-Eyetone meter. the meter does give better results than estimating Dextrostix by eye, but the difference was never statistically significant.", "contents": "The use of dextrostix and dextrostix reflectance meters in the diagnosis of neonatal hypoglycemia. Dextrostix is now widely used as a method of screening for hypoglycemia of the newborn. There has always been some anxiety about its accuracy for estimating very low blood sugars; this is important, since symptomatic hypoglycemia of the newborn does not usually occur until the blood glucose falls below 20 mg/dl. In 1970 a reflectance meter was introduced which would measure the colour of the strip electronically. The aim of this study was to assess the accuracy of the meter and its reliability in estimating hypoglycemia. The study was continued to investigate a new model of the reflectance meter which superseded the original one. In the pilot study using the original model, blood samples were taken from 46 babies. Readings of the Dextrostix were made by two independent observers and compared with a reading taken on the reflectance meter. These estimations were later compared with blood glucose measured by the glucose oxidase method. the meter showed a marked tendency to overestimate the blood glucose: 44 out of 46 samples were overestimated (Fig. 1, 2). In the second series 180 cord blood samples were collected. Because the intention was to study very low levels of blood glucose, the samples were allowed to stand at room temperature for several hours to allow glycolysis to occur. Again, readings were taken by two independent observers and compared with the readings taken on the new Dextrostix-Eyetone meter. The blood glucose was measured on each of the samples. There was a strong correlation (r = 0.8877, p less than 0.00005) between the blood glucose values and the readings taken from the meter, with no tendency towards overestimation (Fig. 3). A similar correlation (r = 0.8533, p less than 0.00005) was seen for the observers' readings and the chemical method, although there was a tendency to underestimate blood glucose (Fig. 4). When the meter gave an estimate of more than 20 ml/dl, in no case was the actual blood glucose in the profoundly hypoglycemic group of less than 10 mg/dl. When estimated by eye there was one case in which the blood glucose was only 7 mg/dl but the observer had estimated the result of 30 mg/dl. In order to avoid any possibility that a blood glucose was less than 20 mg/dl, it is necessary to take action on any estimate below 40 mg/dl on a Dextrostix. Despite this, Dextrostix remains a very useful method of screening for neonatal hypoglycemia, whether assessed by eye or with the new Dextrostix-Eyetone meter. the meter does give better results than estimating Dextrostix by eye, but the difference was never statistically significant.", "PMID": 966130}
{"id": "pubmed23n0032_6582", "title": "A comparative study between the values of lecithin, sphingomyelin, lysolecithin, the L/S index, and the Clements test in amniotic fluid.", "content": "The relationship between the concentration of several phospholipids in amniotic fluid and the ocurrence of neonatal RDS was established some years ago. However the methods used for the determination of those substances are sophisticated and time consuming. They require specalized equipment and take at least 2 or 3 hours. CLEMENTS described a semiquantitative method which overcomes these disadvantages. The present study compared the concentration of lecithin, shingomyelin, lysolecithin and the L/S ratio against the results obtained when processing the same samples with the CLEMENTS Test. the results are expressed in mg/100 ml. for the different phospholipids and the test was evaluated as positive, intermediate or negative. a statistically significant correlation has been found between the values of lecithin, lysolecithin and L/S ratio and the results of the CLEMENTS Test, respectively. No correlation could be found with sphingomyelin (Figs. 1-4). It is concluded that the CLEMENTS Test can be a useful tool as a screening test available to any obstetrical centre.", "contents": "A comparative study between the values of lecithin, sphingomyelin, lysolecithin, the L/S index, and the Clements test in amniotic fluid. The relationship between the concentration of several phospholipids in amniotic fluid and the ocurrence of neonatal RDS was established some years ago. However the methods used for the determination of those substances are sophisticated and time consuming. They require specalized equipment and take at least 2 or 3 hours. CLEMENTS described a semiquantitative method which overcomes these disadvantages. The present study compared the concentration of lecithin, shingomyelin, lysolecithin and the L/S ratio against the results obtained when processing the same samples with the CLEMENTS Test. the results are expressed in mg/100 ml. for the different phospholipids and the test was evaluated as positive, intermediate or negative. a statistically significant correlation has been found between the values of lecithin, lysolecithin and L/S ratio and the results of the CLEMENTS Test, respectively. No correlation could be found with sphingomyelin (Figs. 1-4). It is concluded that the CLEMENTS Test can be a useful tool as a screening test available to any obstetrical centre.", "PMID": 966131}
{"id": "pubmed23n0032_6583", "title": "Oxygen consumption, heart rate, respiratory rate and rectal temperature in healthy newborns during the first two hours after birth.", "content": "A report is made on the continuous registration of oxygen consumption, heart rate, respiratory rate and rectal temperature in 32 neonates during the first two hours of life. A short account of the registration technique is given. A comparative study shows, that oxygen consumption, heart rate and respiratory rate fall during the period of observation, whereas the rectal temperature rises. 26 out of the 32 neonates demonstrated a approximately linear fall of each of the oxygen consumption values during the period of observation. There is a similar relationship, which is statistically verified, between the oxygen uptake and heart rate. Oxygen consumption and heart rate can be correlated with each other after the first 60 minutes of life. This close and direct relationship between the oxygen consumption and the heart rate, an important parameter of the heart and circulatory system, stresses clearly the importance of the continuous registration of oxygen consumption for monitoring the general condition of the neonate. A change from this situation means a risk for the neonate which must be clarified by blood-gas analysis. A continuous registration of oxygen uptake for the surveillance of the neonate includes numerous vital functions and appears to be a more suitable parameter than single parameters which include only partial functions. The application of the method is simple and safe for the infant.", "contents": "Oxygen consumption, heart rate, respiratory rate and rectal temperature in healthy newborns during the first two hours after birth. A report is made on the continuous registration of oxygen consumption, heart rate, respiratory rate and rectal temperature in 32 neonates during the first two hours of life. A short account of the registration technique is given. A comparative study shows, that oxygen consumption, heart rate and respiratory rate fall during the period of observation, whereas the rectal temperature rises. 26 out of the 32 neonates demonstrated a approximately linear fall of each of the oxygen consumption values during the period of observation. There is a similar relationship, which is statistically verified, between the oxygen uptake and heart rate. Oxygen consumption and heart rate can be correlated with each other after the first 60 minutes of life. This close and direct relationship between the oxygen consumption and the heart rate, an important parameter of the heart and circulatory system, stresses clearly the importance of the continuous registration of oxygen consumption for monitoring the general condition of the neonate. A change from this situation means a risk for the neonate which must be clarified by blood-gas analysis. A continuous registration of oxygen uptake for the surveillance of the neonate includes numerous vital functions and appears to be a more suitable parameter than single parameters which include only partial functions. The application of the method is simple and safe for the infant.", "PMID": 966132}
{"id": "pubmed23n0032_6584", "title": "The effect of modern intensive monitoring in obstetrics on infant mortality and the incidence of hypoxia and acidosis.", "content": "We consider intensive monitoring to be fetal monitoring during labor and in the newborn period of all births using the most efficient methods. During the last ten years we have sequentially used the following techniques: Amnioscopy, blood analysis, estrogen determinations in urine, external and internal cardiotokography and internal pressure determinations, gas analyses of umbilical blood. amniotic fluid analyses (phospholipids), ultrasound (B-apparatus) and HCS determinations. All clinics dealing with risk pregnancies should have these techniques available. Total perinatal mortality decreased to below 2% after introduction of cardiotokography. During the last year it decreased to 0.89%. Premature mortality shows the same decrease and is 50% of total mortality. The frequency of premature deliveries remained unchanged at 6.2%. Both improved intensive monitoring and neonatal reanimation and intensive care contributed to the reduction of perinatal mortality. Continuous heart rate recordings make it possible to uncover hypoxic and acidotic states in time and this is of particular value for the premature infant. The incidence of acidosis (pH less than 7.10) was 2.03% before monitoring was introduced and fell to 0.45% this year when intensive monitoring became the rule. No pH lower than 7.0 was found this year. It is thus not sufficient to monitor only cases at risk, since in about 50% of infants born with acidosis no alarming symptoms were found that would have indicated the need for intensive monitoring. Infant mortality should be reduced to less than 1% if the diagnostic tools available are applied. Below this nonviable infants limit further improvement. Perinatal hypoxia and acidosis (below pH 7.10) should also be lower than 0.5% but at least lower than 1%.", "contents": "The effect of modern intensive monitoring in obstetrics on infant mortality and the incidence of hypoxia and acidosis. We consider intensive monitoring to be fetal monitoring during labor and in the newborn period of all births using the most efficient methods. During the last ten years we have sequentially used the following techniques: Amnioscopy, blood analysis, estrogen determinations in urine, external and internal cardiotokography and internal pressure determinations, gas analyses of umbilical blood. amniotic fluid analyses (phospholipids), ultrasound (B-apparatus) and HCS determinations. All clinics dealing with risk pregnancies should have these techniques available. Total perinatal mortality decreased to below 2% after introduction of cardiotokography. During the last year it decreased to 0.89%. Premature mortality shows the same decrease and is 50% of total mortality. The frequency of premature deliveries remained unchanged at 6.2%. Both improved intensive monitoring and neonatal reanimation and intensive care contributed to the reduction of perinatal mortality. Continuous heart rate recordings make it possible to uncover hypoxic and acidotic states in time and this is of particular value for the premature infant. The incidence of acidosis (pH less than 7.10) was 2.03% before monitoring was introduced and fell to 0.45% this year when intensive monitoring became the rule. No pH lower than 7.0 was found this year. It is thus not sufficient to monitor only cases at risk, since in about 50% of infants born with acidosis no alarming symptoms were found that would have indicated the need for intensive monitoring. Infant mortality should be reduced to less than 1% if the diagnostic tools available are applied. Below this nonviable infants limit further improvement. Perinatal hypoxia and acidosis (below pH 7.10) should also be lower than 0.5% but at least lower than 1%.", "PMID": 966133}
{"id": "pubmed23n0032_6585", "title": "Ultrasound and biochemical findings in intrauterine growth retardation.", "content": "An attempt was made to make an early diagnosis of intrauterine growth retardation due to chronic placental insufficiency using ultrasound cephalometry and biochemical tests of placental function. We examined 83 hospitalised patients in whom there was a risk or suspicion of chronic placental insufficiency. For each patient an average of 4 determinations were made of head diameter, 10 of estriol and 3 of pregnandiol urinary excretion, 6 serum HPL and 7 of heat stable alkaline phosphatase (HSAP). Fetal growth retardation was assumed if the last 2 determined head diameters were below the normal curve with the same tendency and with term being well predicted. Biochemical parameters were considered to indicate pathological changes if they were 95% below normal values, with 2 below this range or 3 continuously falling below the normal level. Infants were assessed after birth both neurologically and somatically according to DUBOWITZ. Small for date infants were those whose birth weight was below the 10th percentile (LUBCHENKO). This was found to be the case in 15 out of 83 newborns five of whom were younger than 37 weeks. These 15 could be diagnosed before birth with various degrees of certainty. For estriol and HPL this was 67% or 53% and these two parameters were found to be the most valuable. Cephalometry was found to be less valuable with 20%, pregnandiol levels with 9% and placental HSAP with 33%. Hence it is recommended to perform serial determinations of estriol and HPL in the third trimester of pregnancy in all patients with histories indicative of fetal growth retardation and in those in whom the uterus appears small for date.", "contents": "Ultrasound and biochemical findings in intrauterine growth retardation. An attempt was made to make an early diagnosis of intrauterine growth retardation due to chronic placental insufficiency using ultrasound cephalometry and biochemical tests of placental function. We examined 83 hospitalised patients in whom there was a risk or suspicion of chronic placental insufficiency. For each patient an average of 4 determinations were made of head diameter, 10 of estriol and 3 of pregnandiol urinary excretion, 6 serum HPL and 7 of heat stable alkaline phosphatase (HSAP). Fetal growth retardation was assumed if the last 2 determined head diameters were below the normal curve with the same tendency and with term being well predicted. Biochemical parameters were considered to indicate pathological changes if they were 95% below normal values, with 2 below this range or 3 continuously falling below the normal level. Infants were assessed after birth both neurologically and somatically according to DUBOWITZ. Small for date infants were those whose birth weight was below the 10th percentile (LUBCHENKO). This was found to be the case in 15 out of 83 newborns five of whom were younger than 37 weeks. These 15 could be diagnosed before birth with various degrees of certainty. For estriol and HPL this was 67% or 53% and these two parameters were found to be the most valuable. Cephalometry was found to be less valuable with 20%, pregnandiol levels with 9% and placental HSAP with 33%. Hence it is recommended to perform serial determinations of estriol and HPL in the third trimester of pregnancy in all patients with histories indicative of fetal growth retardation and in those in whom the uterus appears small for date.", "PMID": 966134}
{"id": "pubmed23n0032_6586", "title": "Estrogens, lactation and oral glucose tolerance test in the early puerperium.", "content": "An oral glucose tolerance test (OGTT) was performed on 98 women free from any known risk factor of diabetes on the fifth day of the puerperium. Results show that OGTT is greatly influenced by the conditions of lactation. A high proportion of abnormal curves (50%) is found among the group of women receiving estrogens as lactation suppressors at the time of the test. However, in breast feeding women or in non breast feeding women not given estrogen, the proportion of abnormal curves is less than 10%. It is concluded that the unexplained previously reported lack of specificity of the OGTT in the early puerperium could be related to hormonal treatment for lactation suppression.", "contents": "Estrogens, lactation and oral glucose tolerance test in the early puerperium. An oral glucose tolerance test (OGTT) was performed on 98 women free from any known risk factor of diabetes on the fifth day of the puerperium. Results show that OGTT is greatly influenced by the conditions of lactation. A high proportion of abnormal curves (50%) is found among the group of women receiving estrogens as lactation suppressors at the time of the test. However, in breast feeding women or in non breast feeding women not given estrogen, the proportion of abnormal curves is less than 10%. It is concluded that the unexplained previously reported lack of specificity of the OGTT in the early puerperium could be related to hormonal treatment for lactation suppression.", "PMID": 966135}
{"id": "pubmed23n0032_6587", "title": "Age and emotion of an offender as determinants of adult punitive reactions.", "content": "Male and female college students were shown videotapes of supposed male offenders describing the details of thefts they had committed. Videotaped sequences were varied so that offenders were 9 to 10 years old or of college age. Also, actors varied in their mode of emotional self-presentation. Comparisons of punishments suggested by subjects indicated that joyful actors received the highest level of punishment while distressed offenders received the lowest level of suggested punishment. It was also found that the emotions of children exerted a greater persuasive effect on subjects' ratings than did the emotions of adults although the pattern of results was similar across ages. These results were discussed as demonstrating that the age and emotion of an offender can exert considerable influence on the attribution of blame made by a disciplinarian.", "contents": "Age and emotion of an offender as determinants of adult punitive reactions. Male and female college students were shown videotapes of supposed male offenders describing the details of thefts they had committed. Videotaped sequences were varied so that offenders were 9 to 10 years old or of college age. Also, actors varied in their mode of emotional self-presentation. Comparisons of punishments suggested by subjects indicated that joyful actors received the highest level of punishment while distressed offenders received the lowest level of suggested punishment. It was also found that the emotions of children exerted a greater persuasive effect on subjects' ratings than did the emotions of adults although the pattern of results was similar across ages. These results were discussed as demonstrating that the age and emotion of an offender can exert considerable influence on the attribution of blame made by a disciplinarian.", "PMID": 966136}
{"id": "pubmed23n0032_6588", "title": "Casual attributions for success and failure for psychiatric rehabilitees and college students.", "content": "College students and psychiatric rehabilitees performed a manual dexterity task in which consistent success or failure was maipulated over four consecutive task trials. Contrasting predictions for the use of casual attributions (luck, task difficulty, effort, and ability) following Trial 1 and Trial 4 for the two populations were derived from Heider's balance theory and \"naive theory of action\". Consistent with native theory, students who succeeded and rehabilitees who succeeded used unstable attributions only college students who failed used stable attributions to account for these trial outcomes, whereas students who failed and rehabilitees who failed made significant changes in their attributional patterns from Trial 1 to Trial 4. Results are discussed regarding intervention with psychiatric rehabilitees and other groups with severe achievement difficulties.", "contents": "Casual attributions for success and failure for psychiatric rehabilitees and college students. College students and psychiatric rehabilitees performed a manual dexterity task in which consistent success or failure was maipulated over four consecutive task trials. Contrasting predictions for the use of casual attributions (luck, task difficulty, effort, and ability) following Trial 1 and Trial 4 for the two populations were derived from Heider's balance theory and \"naive theory of action\". Consistent with native theory, students who succeeded and rehabilitees who succeeded used unstable attributions only college students who failed used stable attributions to account for these trial outcomes, whereas students who failed and rehabilitees who failed made significant changes in their attributional patterns from Trial 1 to Trial 4. Results are discussed regarding intervention with psychiatric rehabilitees and other groups with severe achievement difficulties.", "PMID": 966137}
{"id": "pubmed23n0032_6589", "title": "Effects of colestipol hydrochloride on drug absorption in the rat II.", "content": "The effects of colestipol hydrochloride, a hypocholesterolemic bile acid-binding anion-exchange polymer, on the GI absorption of drugs commonly used in humans were studied in the rat. Colestipol hydrochloride was given by gavage in single doses of 71.5 or 214.5 mg/kg, equivalent to 5 or 15 g, respectively, in a 70-kg human; controls received equivalent amounts of microcrystalline cellulose. Single oral doses of labeled drugs were given concurrently with colestipol hydrochloride or microcrystalline cellulose at the human therapeutic dose range on a milligrams per kilogram basis. Subsequent changes in serum drug levels were measured at several time periods, and absorption was evaluated as the total area under the time-concentration curve. Colestipol hydrochloride at either dose did not significantly alter the absorption of 6-14C-nicotinic acid, 7-3H-tetracycline, 35S-chlorpromazine, 12alpha-3H-digoxin, warfarin (alpha-14C-benzyl), or clofibrate (14C-carboxyl). In addition, the effects of 214.5 mg/kg of colestipol hydrochloride were compared with the same dose of cholestyramine with respect to the absorption of 3-14C-hydrochlorothiazide, 2-14C-phenobarbital, and 3H-digitoxin. Cholestyramine reduced absorption of hydrochlorothiazide by 42%, but colestipol hydrochloride had no significant effect. Neither resin altered phenobarbital or digitoxin absorption when compared with the the control, but a significant difference occurred between the two resins with digitoxin; areas under the time-concentration curve [in (dpm/0.1 ml serum) x hr] were: colestipol hydrochloride, 2001; cholestyramine, 16,300; and cellulose, 17, 067. These results indicate that colestipol hydrochloride and cholestyramine can differ in their effects on the absorption of certain drugs from the GI tract of the rat.", "contents": "Effects of colestipol hydrochloride on drug absorption in the rat II. The effects of colestipol hydrochloride, a hypocholesterolemic bile acid-binding anion-exchange polymer, on the GI absorption of drugs commonly used in humans were studied in the rat. Colestipol hydrochloride was given by gavage in single doses of 71.5 or 214.5 mg/kg, equivalent to 5 or 15 g, respectively, in a 70-kg human; controls received equivalent amounts of microcrystalline cellulose. Single oral doses of labeled drugs were given concurrently with colestipol hydrochloride or microcrystalline cellulose at the human therapeutic dose range on a milligrams per kilogram basis. Subsequent changes in serum drug levels were measured at several time periods, and absorption was evaluated as the total area under the time-concentration curve. Colestipol hydrochloride at either dose did not significantly alter the absorption of 6-14C-nicotinic acid, 7-3H-tetracycline, 35S-chlorpromazine, 12alpha-3H-digoxin, warfarin (alpha-14C-benzyl), or clofibrate (14C-carboxyl). In addition, the effects of 214.5 mg/kg of colestipol hydrochloride were compared with the same dose of cholestyramine with respect to the absorption of 3-14C-hydrochlorothiazide, 2-14C-phenobarbital, and 3H-digitoxin. Cholestyramine reduced absorption of hydrochlorothiazide by 42%, but colestipol hydrochloride had no significant effect. Neither resin altered phenobarbital or digitoxin absorption when compared with the the control, but a significant difference occurred between the two resins with digitoxin; areas under the time-concentration curve [in (dpm/0.1 ml serum) x hr] were: colestipol hydrochloride, 2001; cholestyramine, 16,300; and cellulose, 17, 067. These results indicate that colestipol hydrochloride and cholestyramine can differ in their effects on the absorption of certain drugs from the GI tract of the rat.", "PMID": 966139}
{"id": "pubmed23n0032_6590", "title": "Effects of film coatings on tablet hardness.", "content": "The effects of five conventional film-coating materials on tablet hardness were studied. Placebos showed apparently linear increases in hardness as coatings were applied. Completely coated samples exhibited hardness increases from 50 to 140%, with a corresponding 3% increase in tablet weight. Equations were derived relating hardness changes to the \"breaking strength\" of the film on the tablet. Findings indicate that the coatings exert their influence primarily along the diameter of the tablet in a direction perpendicular to an applied compressional force. Furthermore, the coating process itself did not alter core hardness since tablets from which the film could be stripped showed original values.", "contents": "Effects of film coatings on tablet hardness. The effects of five conventional film-coating materials on tablet hardness were studied. Placebos showed apparently linear increases in hardness as coatings were applied. Completely coated samples exhibited hardness increases from 50 to 140%, with a corresponding 3% increase in tablet weight. Equations were derived relating hardness changes to the \"breaking strength\" of the film on the tablet. Findings indicate that the coatings exert their influence primarily along the diameter of the tablet in a direction perpendicular to an applied compressional force. Furthermore, the coating process itself did not alter core hardness since tablets from which the film could be stripped showed original values.", "PMID": 966140}
{"id": "pubmed23n0032_6591", "title": "Quantitative precorneal disposition of topically applied pilocarpine nitrate in rabbit eyes.", "content": "The present study was designed to quantitate the influence of several precorneal factors on the disposition of topically applied ophthalmic drugs. With tritiated pilocarpine nitrate methodology was developed for in vivo assessment of the relative contribution of tear turnover, instilled solution drainage, and nonproductive absorption to the loss of drug from the precorneal area. Studies were conducted in both awake and anesthetized rabbits whose drainage ducts were either unobstructed or plugged, and the loss of drug was monitored directly from the precorneal area or as appearance in the aqueous humor. By selective variation in experimental conditions, the influence of tear turnover, instilled solution drainage, and nonproductive absorption on ocular drug bioavailability was separately studied and quantitated. Instilled solution drainage was by far the largest contributing factor in the loss of drug from the precorneal area of the eye and, in the range of instilled volumes normally employed, tear turnover played a relatively minor role in drug loss. Compared to the cornea, precorneal tissue other than the cornea has a considerably greater surface area and thus is a potentially signifanct route for drug loss. However, under normal circumstances, loss by this route was minimal as compared to loss via instilled solution drainage.", "contents": "Quantitative precorneal disposition of topically applied pilocarpine nitrate in rabbit eyes. The present study was designed to quantitate the influence of several precorneal factors on the disposition of topically applied ophthalmic drugs. With tritiated pilocarpine nitrate methodology was developed for in vivo assessment of the relative contribution of tear turnover, instilled solution drainage, and nonproductive absorption to the loss of drug from the precorneal area. Studies were conducted in both awake and anesthetized rabbits whose drainage ducts were either unobstructed or plugged, and the loss of drug was monitored directly from the precorneal area or as appearance in the aqueous humor. By selective variation in experimental conditions, the influence of tear turnover, instilled solution drainage, and nonproductive absorption on ocular drug bioavailability was separately studied and quantitated. Instilled solution drainage was by far the largest contributing factor in the loss of drug from the precorneal area of the eye and, in the range of instilled volumes normally employed, tear turnover played a relatively minor role in drug loss. Compared to the cornea, precorneal tissue other than the cornea has a considerably greater surface area and thus is a potentially signifanct route for drug loss. However, under normal circumstances, loss by this route was minimal as compared to loss via instilled solution drainage.", "PMID": 966141}
{"id": "pubmed23n0032_6592", "title": "Substitued 5-nitro-1, 3-dioxanes: correlation of chemical structure and antimicrobial activity.", "content": "Various derivatives of 5-nitro-1, 3-dioxane were synthesized to determine the relative effect of chemical substitution in the 2-and 5-positions on broad spectrum antimicrobial activity. Each compound was evaluated quantitatively by calculation of a microbiocidal index, which measured the time to kill several different microorganisms. This test system indicated that 5-bromo-5-nitro substitution was essential for significant activity. Optimal activity was effected by 2-methyl substitution in the alkyl series and 2-hydroxy-phenyl substitution in the aryl series. The antimicrobial activity of the substituted dioxanes was not related directly to water solubility or hydrolysis to microbiocidal diols or aldehydes.", "contents": "Substitued 5-nitro-1, 3-dioxanes: correlation of chemical structure and antimicrobial activity. Various derivatives of 5-nitro-1, 3-dioxane were synthesized to determine the relative effect of chemical substitution in the 2-and 5-positions on broad spectrum antimicrobial activity. Each compound was evaluated quantitatively by calculation of a microbiocidal index, which measured the time to kill several different microorganisms. This test system indicated that 5-bromo-5-nitro substitution was essential for significant activity. Optimal activity was effected by 2-methyl substitution in the alkyl series and 2-hydroxy-phenyl substitution in the aryl series. The antimicrobial activity of the substituted dioxanes was not related directly to water solubility or hydrolysis to microbiocidal diols or aldehydes.", "PMID": 966142}
{"id": "pubmed23n0032_6593", "title": "GLC and NMR analysis of isomeric impurities in the new anti-inflammatory agent benoxaprofen.", "content": "GLC and NMR methods are described for the determination of four possible isomeric impurities in the novel anti-inflammatory agent benoxaprofen. The 2- and 3-chlorophenyl isomers were determined by GLC after alkaline hydrolysis and subsequent methylation. A rapid NMR procedure, using the lanthanide shift reagent tris- (1, 1, 1, 2, 2, 3, 3-heptafluoro- 7, 7-dimethyl- 4, 6-octanedionato)-europium, was developed for the 6- and 7- (alpha-methylacetic acid) isomers. Similar methodolology, with tris-(3-heptafluorobutyryl-d-camphorato)europium, enabled the determination of the enantiomer ratio for benoxaprofen. For the positional isomers, the limits of detection were 0.05% by GLC and 0.2% by NMR.", "contents": "GLC and NMR analysis of isomeric impurities in the new anti-inflammatory agent benoxaprofen. GLC and NMR methods are described for the determination of four possible isomeric impurities in the novel anti-inflammatory agent benoxaprofen. The 2- and 3-chlorophenyl isomers were determined by GLC after alkaline hydrolysis and subsequent methylation. A rapid NMR procedure, using the lanthanide shift reagent tris- (1, 1, 1, 2, 2, 3, 3-heptafluoro- 7, 7-dimethyl- 4, 6-octanedionato)-europium, was developed for the 6- and 7- (alpha-methylacetic acid) isomers. Similar methodolology, with tris-(3-heptafluorobutyryl-d-camphorato)europium, enabled the determination of the enantiomer ratio for benoxaprofen. For the positional isomers, the limits of detection were 0.05% by GLC and 0.2% by NMR.", "PMID": 966143}
{"id": "pubmed23n0032_6594", "title": "Fluphenazine enanthate and fluphenazine decanoate: intramuscular injection and esterification as requirements for slow - release characteristics in dogs.", "content": "14C-Fluphenazine base was administered intramuscularly in sesame oil to five male beagles (2 mg/kg). The concentration of radioactivity in plasma and the excretion of radioactivity in urine and feces were measured for 14 days. Maximum concentrations of radioactivity were found in plasma 2 hr after administration. These levels declined with elimination half-lives of 3.20 hr during the 2-12-hr interval after dosing and of 4.02 days during the 2-14-day interval. Most administered radioactivity was excreted during the first 2 days after dosing, predominantly in the feces. An average of 0.43% of the dose was present at the injection site 14 days after dosing; some residual radioactivity was found in the liver and in the ocular portion consisting of the combined retina, choroid, and sclera. 14C-Fluphenazine and its enanthate and decanoate esters were each administered intravenously to three different groups of intact dogs at doses of 1 mg/kg. Regardless of which compound was administered, concentrations of radioactivity in the plasma of these dogs were comparable. Thirty minutes after these dogs had been dosed with 14C-fluphenazine enanthate or 14C-fluphenazine decanoate, most radioactivity in the plasma was present as 14C-fluphenazine base and other unidentified metabolites; at this time, at least 79% of either of the two 14C-fluphenazine esters had been biotransformed. The excretion of radioactivity by these same three groups of dogs was very similar, regardless of which of the compounds was given. In 7 days, an average of only 3-4% of the dose was excreted in urine; the remainder was excreted in feces. 14C Fluphenazine and its enanthate and decanoate esters (1 mg/kg) were administered intravenously to dogs whose bile ducts had been cannulated. The amounts of radioactivity excreted in the urine and bile in 8 hr were very similar, as were the residual amounts of radioactivity present in selected tissues. Comparison of the data obtained from dogs given these three compounds intravenously (unformulated) or intramuscularly in sesame oil points to the following conclusions: (a) fluphenazine base per se does not provide slow-release characteristics unless it has been esterified, for example, with heptanoic or decanoic acid, and (b) intramuscular rather than intravenous administration of these two esters is responsible for producing their slow-release characteristics.", "contents": "Fluphenazine enanthate and fluphenazine decanoate: intramuscular injection and esterification as requirements for slow - release characteristics in dogs. 14C-Fluphenazine base was administered intramuscularly in sesame oil to five male beagles (2 mg/kg). The concentration of radioactivity in plasma and the excretion of radioactivity in urine and feces were measured for 14 days. Maximum concentrations of radioactivity were found in plasma 2 hr after administration. These levels declined with elimination half-lives of 3.20 hr during the 2-12-hr interval after dosing and of 4.02 days during the 2-14-day interval. Most administered radioactivity was excreted during the first 2 days after dosing, predominantly in the feces. An average of 0.43% of the dose was present at the injection site 14 days after dosing; some residual radioactivity was found in the liver and in the ocular portion consisting of the combined retina, choroid, and sclera. 14C-Fluphenazine and its enanthate and decanoate esters were each administered intravenously to three different groups of intact dogs at doses of 1 mg/kg. Regardless of which compound was administered, concentrations of radioactivity in the plasma of these dogs were comparable. Thirty minutes after these dogs had been dosed with 14C-fluphenazine enanthate or 14C-fluphenazine decanoate, most radioactivity in the plasma was present as 14C-fluphenazine base and other unidentified metabolites; at this time, at least 79% of either of the two 14C-fluphenazine esters had been biotransformed. The excretion of radioactivity by these same three groups of dogs was very similar, regardless of which of the compounds was given. In 7 days, an average of only 3-4% of the dose was excreted in urine; the remainder was excreted in feces. 14C Fluphenazine and its enanthate and decanoate esters (1 mg/kg) were administered intravenously to dogs whose bile ducts had been cannulated. The amounts of radioactivity excreted in the urine and bile in 8 hr were very similar, as were the residual amounts of radioactivity present in selected tissues. Comparison of the data obtained from dogs given these three compounds intravenously (unformulated) or intramuscularly in sesame oil points to the following conclusions: (a) fluphenazine base per se does not provide slow-release characteristics unless it has been esterified, for example, with heptanoic or decanoic acid, and (b) intramuscular rather than intravenous administration of these two esters is responsible for producing their slow-release characteristics.", "PMID": 966144}
{"id": "pubmed23n0032_6595", "title": "GLC determination of aprindine: quantitation and stability measurement.", "content": "A GLC method of analysis of a new antiarrhythmic agent, aprindine, is described. The raw material of the new drug substance, supplied as the hydrochloride salt, is dissolved in deionized water, and the base is liberated by a 10% aqueous solution of sodium carbonate. aprindine is extracted with chloroform and mixed with the internal standard, 5alpha-cholestane. GLC is perfomed on a glass column packed with 3.8% W-98 on Chromosorb W-HP. Quantitation is achieved by computer calculation of the peak area ratios. GLC-mass spectral analysis indicates that the observed peak is that of aprindine, with a molecular ion at m/e 322. The retention times of aprindine and the internal standard are 2.0 and 5.8 min, respectively. All synthetic precursors show a shorter retention time than aprindine. This GLC method is applied to the quantitative determination of aprindine as raw material and in capsule and ampul formulations. The method is also used to measure the stability of aprindine to acid, base, dry, heat, refluxing, and UV light and to pH variations.", "contents": "GLC determination of aprindine: quantitation and stability measurement. A GLC method of analysis of a new antiarrhythmic agent, aprindine, is described. The raw material of the new drug substance, supplied as the hydrochloride salt, is dissolved in deionized water, and the base is liberated by a 10% aqueous solution of sodium carbonate. aprindine is extracted with chloroform and mixed with the internal standard, 5alpha-cholestane. GLC is perfomed on a glass column packed with 3.8% W-98 on Chromosorb W-HP. Quantitation is achieved by computer calculation of the peak area ratios. GLC-mass spectral analysis indicates that the observed peak is that of aprindine, with a molecular ion at m/e 322. The retention times of aprindine and the internal standard are 2.0 and 5.8 min, respectively. All synthetic precursors show a shorter retention time than aprindine. This GLC method is applied to the quantitative determination of aprindine as raw material and in capsule and ampul formulations. The method is also used to measure the stability of aprindine to acid, base, dry, heat, refluxing, and UV light and to pH variations.", "PMID": 966145}
{"id": "pubmed23n0032_6596", "title": "Comparative plasma concentrations of quinidine following administration of one intramuscular and three oral formulations to 13 human subjects.", "content": "A GLC method, based on flame-ionization detection, was developed for the assay of methotrimeprazine and its sulfoxide in plasma. For a 6-ml aliquot, the sensitivity was 2-3 ng/ml for the unchanged drug and 4-5 ng/ml for the sulfoxide. The coefficient of variation, calculated from duplicate analyses of plasma samples, was 8-15% for concentrations between 10 and 100 ng/ml. Patients treated with orally administered methotrimeprazine had higher plasma levels of the sulfoxide than of unmetabolized drug. The method also was applied to the analysis of promazine and chlorpromazine in patient plasma.", "contents": "Comparative plasma concentrations of quinidine following administration of one intramuscular and three oral formulations to 13 human subjects. A GLC method, based on flame-ionization detection, was developed for the assay of methotrimeprazine and its sulfoxide in plasma. For a 6-ml aliquot, the sensitivity was 2-3 ng/ml for the unchanged drug and 4-5 ng/ml for the sulfoxide. The coefficient of variation, calculated from duplicate analyses of plasma samples, was 8-15% for concentrations between 10 and 100 ng/ml. Patients treated with orally administered methotrimeprazine had higher plasma levels of the sulfoxide than of unmetabolized drug. The method also was applied to the analysis of promazine and chlorpromazine in patient plasma.", "PMID": 966146}
{"id": "pubmed23n0032_6597", "title": "GLC determination of methotrimeprazine and its sulfoxide in plasma.", "content": "A GLC method, based on flame-ionization detection, was developed for the assay of methotrimeprazine and its sulfoxide in plasma. For a 6-ml aliquot, the sensitivity was 2-3 ng/ml for the unchanged drug and 4-5 ng/ml for the sulfoxide. The coefficient of variation, calculated from duplicate analyses of plasma samples, was 8-15% for concentrations between 10 and 100 ng/ml. Patients treated with orally administered methotrimeprazine had higher plasma levels of the sulfoxide than of unmetabolized drug. The method also was applied to the analysis of promazine and chlorpromazine in patient plasma.", "contents": "GLC determination of methotrimeprazine and its sulfoxide in plasma. A GLC method, based on flame-ionization detection, was developed for the assay of methotrimeprazine and its sulfoxide in plasma. For a 6-ml aliquot, the sensitivity was 2-3 ng/ml for the unchanged drug and 4-5 ng/ml for the sulfoxide. The coefficient of variation, calculated from duplicate analyses of plasma samples, was 8-15% for concentrations between 10 and 100 ng/ml. Patients treated with orally administered methotrimeprazine had higher plasma levels of the sulfoxide than of unmetabolized drug. The method also was applied to the analysis of promazine and chlorpromazine in patient plasma.", "PMID": 966147}
{"id": "pubmed23n0032_6598", "title": "Drug release from methyl acrylate-methyl methacrylate copolymer matrix III: stimultaneous release of noninteracting drug-excipient mixtures.", "content": "The simultaneous release of mixtures of noninteracting chemicals incorporated in a methyl acrylate-methyl methacrylate copolymer was studied. Different mixtures of dextrose-methapyrilene hydrochloride and sodium chloride-methapyrilene hydrochlodire in the plastic were compressed into tablets, and single-surface release was obtained in 0.N HCI at 37 degrees. The amounts of both drug and excipients released per unit surface rate constants were analysed in terms of the solubility, diffusion coefficient, concentrations of each chemical, and porosity and tortuosity of the tablets. It was concluded that for each tablet system the individual solid-liquid boundaries of the incorporated chemicals had merged together, and the release could be explained by Higuchi's square foot of time relationship.", "contents": "Drug release from methyl acrylate-methyl methacrylate copolymer matrix III: stimultaneous release of noninteracting drug-excipient mixtures. The simultaneous release of mixtures of noninteracting chemicals incorporated in a methyl acrylate-methyl methacrylate copolymer was studied. Different mixtures of dextrose-methapyrilene hydrochloride and sodium chloride-methapyrilene hydrochlodire in the plastic were compressed into tablets, and single-surface release was obtained in 0.N HCI at 37 degrees. The amounts of both drug and excipients released per unit surface rate constants were analysed in terms of the solubility, diffusion coefficient, concentrations of each chemical, and porosity and tortuosity of the tablets. It was concluded that for each tablet system the individual solid-liquid boundaries of the incorporated chemicals had merged together, and the release could be explained by Higuchi's square foot of time relationship.", "PMID": 966148}
{"id": "pubmed23n0032_6599", "title": "Binding of spirolactones to human plasma proteins.", "content": "The lipophilicity and plasma binding of 16 spirolactones and 4 hydroxy acid analogs were determined. Mathematical expressions were derived to correlate quantitatively the extent of plasma binding to the lipophilicity of the drugs. The nonspecific binding of these spirolactones and their hydroxy acid analogs was also analyzed using purified serum albumin. A computer program was developed to examine the mechanism of drug-serum protein interactions. One class of binding sites was observed for the range of concentrations used. The number of binding sites and the equilibrium binding constant were computed and were sensitive to substitution at the C-6 and C7 positions. Hydrolysis of the C-17 lactone ring in spirolactones to form hydroxy acid analogs resulted in a decrease in the lipophilicity and, hence, the equilibrium constant for binding.", "contents": "Binding of spirolactones to human plasma proteins. The lipophilicity and plasma binding of 16 spirolactones and 4 hydroxy acid analogs were determined. Mathematical expressions were derived to correlate quantitatively the extent of plasma binding to the lipophilicity of the drugs. The nonspecific binding of these spirolactones and their hydroxy acid analogs was also analyzed using purified serum albumin. A computer program was developed to examine the mechanism of drug-serum protein interactions. One class of binding sites was observed for the range of concentrations used. The number of binding sites and the equilibrium binding constant were computed and were sensitive to substitution at the C-6 and C7 positions. Hydrolysis of the C-17 lactone ring in spirolactones to form hydroxy acid analogs resulted in a decrease in the lipophilicity and, hence, the equilibrium constant for binding.", "PMID": 966149}
{"id": "pubmed23n0032_6600", "title": "Rationale for apparent differences in pharmacokinetic aspects of model compounds determined from blood level data and urinary excretion data in rats.", "content": "Results of studies carried out in rats for model compounds, D-(-) mandelic acid, benzoylformic acid, and some of their para-alkylated homologs, showed that their biological half-lives determined from the elimination phase of urinary excretion data were longer than those determined from the elimination phase of blood level data. With compounds that followed multicompartment open models, the initial distributive phase (alpha-phase) noted from the blood level data was not detected from the urinary excretion data. Based on the analysis of half-life data obtained in the absence and presence of DL-tropic acid (a competitive renal tubular secretion inhibitor of these compounds), it is proposed that, besides the shortness of the alpha-phase period, the factor accounting for these compounds is their retention and/or detention in the renal tubular membranes during their tubular secretion. Furthermore, it is proposed that the renal tubular membranes do not constitute a part of the central or peripheral compartment. Examples are cited to show that, where studies are reported for the same drugs in the same human subjects in the same laboratory, drugs usually exhibit longer biological half-lives when urinary excretion data rather than blood level data are used.", "contents": "Rationale for apparent differences in pharmacokinetic aspects of model compounds determined from blood level data and urinary excretion data in rats. Results of studies carried out in rats for model compounds, D-(-) mandelic acid, benzoylformic acid, and some of their para-alkylated homologs, showed that their biological half-lives determined from the elimination phase of urinary excretion data were longer than those determined from the elimination phase of blood level data. With compounds that followed multicompartment open models, the initial distributive phase (alpha-phase) noted from the blood level data was not detected from the urinary excretion data. Based on the analysis of half-life data obtained in the absence and presence of DL-tropic acid (a competitive renal tubular secretion inhibitor of these compounds), it is proposed that, besides the shortness of the alpha-phase period, the factor accounting for these compounds is their retention and/or detention in the renal tubular membranes during their tubular secretion. Furthermore, it is proposed that the renal tubular membranes do not constitute a part of the central or peripheral compartment. Examples are cited to show that, where studies are reported for the same drugs in the same human subjects in the same laboratory, drugs usually exhibit longer biological half-lives when urinary excretion data rather than blood level data are used.", "PMID": 966150}
{"id": "pubmed23n0032_6601", "title": "Influence of cholestasis on drug elimination: pharmacokinetics.", "content": "A two-compartment model representing the body and the GI tract, with elimination occurring in each compartment, was used to study, in theory, the influence of impaired biliary excretion on drug disposition. The results suggest that cholestasis can either increase or decrease a drug's half-life, depending upon the relative values of the two elimination rate constants, In all cases, however, impaired biliary excretion reduced the initial elimination of drug from the body and increased the half-life of the alpha-phase of drug disposition.", "contents": "Influence of cholestasis on drug elimination: pharmacokinetics. A two-compartment model representing the body and the GI tract, with elimination occurring in each compartment, was used to study, in theory, the influence of impaired biliary excretion on drug disposition. The results suggest that cholestasis can either increase or decrease a drug's half-life, depending upon the relative values of the two elimination rate constants, In all cases, however, impaired biliary excretion reduced the initial elimination of drug from the body and increased the half-life of the alpha-phase of drug disposition.", "PMID": 966151}
{"id": "pubmed23n0032_6602", "title": "Biliary secretion of methotrexate in rats and its inhibition by probenecid.", "content": "The biliary secretion of methotrexate was investigated in rats under steady-state conditions. The transport system involved was saturable and displayed Michaelis-Menten-type kinetics. Values for the maximal rate of transport and a transport constant analogous to the Michaelis constant were 12 mg/hr and 32 mg/liter (7 x 10-5 M), respectively. The inhibition of this transport mechanism by probenecid also was investigated, and the relationship between the plasma concentration of probenecid and the biliary clearance of methotrexate was elucidated. The value of Ki, the dissociation constant for the transport carrier-inhibitor complex, was 23 mug/ml (8 x10-5 M).", "contents": "Biliary secretion of methotrexate in rats and its inhibition by probenecid. The biliary secretion of methotrexate was investigated in rats under steady-state conditions. The transport system involved was saturable and displayed Michaelis-Menten-type kinetics. Values for the maximal rate of transport and a transport constant analogous to the Michaelis constant were 12 mg/hr and 32 mg/liter (7 x 10-5 M), respectively. The inhibition of this transport mechanism by probenecid also was investigated, and the relationship between the plasma concentration of probenecid and the biliary clearance of methotrexate was elucidated. The value of Ki, the dissociation constant for the transport carrier-inhibitor complex, was 23 mug/ml (8 x10-5 M).", "PMID": 966152}
{"id": "pubmed23n0032_6603", "title": "GLC determination of nylidrin in human urine samples.", "content": "A method for the detection of nanogram quantities of nylidrin in human urine is described. The method involves beta-glucuronidase hydrolysis, extraction with chloroform, derivatization by silylation, and GLC determination. The suitability of the method was tested by analysis of urine samples of subjects after oral ingestion of nylidrin hydrochloride.", "contents": "GLC determination of nylidrin in human urine samples. A method for the detection of nanogram quantities of nylidrin in human urine is described. The method involves beta-glucuronidase hydrolysis, extraction with chloroform, derivatization by silylation, and GLC determination. The suitability of the method was tested by analysis of urine samples of subjects after oral ingestion of nylidrin hydrochloride.", "PMID": 966153}
{"id": "pubmed23n0032_6604", "title": "Assay of sulfonylureas in human plasma by high-performance liquid chromatography.", "content": "A sensitive and specific high-performance liquid chromatographic procedure for the determination of chlorpropamide or tolbutamide in plasma in the presence of their metabolites is described. The ether extract of acidified plasma is redissolved in the mobile phase, 17% acetonitrile in 0.05 M aqueous ammonium formate, and chromatographed on a reverse-phase column on a high-performance liquid chromatograph fitted with a UV absorbance detector. Quantitation of plasma samples containing less than 0.5 mug/ml of chlorpropamide and 5 mug/ml of tolbutamide is reported, using these drugs as mutual internal standards. The retention times of the metabolites are such that they do not interfere in the procedure. The assay method was tested in a human volunteer with both drugs and found suitable for single-dose pharmacokinetic studies.", "contents": "Assay of sulfonylureas in human plasma by high-performance liquid chromatography. A sensitive and specific high-performance liquid chromatographic procedure for the determination of chlorpropamide or tolbutamide in plasma in the presence of their metabolites is described. The ether extract of acidified plasma is redissolved in the mobile phase, 17% acetonitrile in 0.05 M aqueous ammonium formate, and chromatographed on a reverse-phase column on a high-performance liquid chromatograph fitted with a UV absorbance detector. Quantitation of plasma samples containing less than 0.5 mug/ml of chlorpropamide and 5 mug/ml of tolbutamide is reported, using these drugs as mutual internal standards. The retention times of the metabolites are such that they do not interfere in the procedure. The assay method was tested in a human volunteer with both drugs and found suitable for single-dose pharmacokinetic studies.", "PMID": 966154}
{"id": "pubmed23n0032_6605", "title": "Effects of intravenous dantrolene sodium on respiratory and cardiovascular functions.", "content": "Dantrolene sodium, a peripherally acting skeletal muscle relaxant, at doses up to 30 mg/kg iv had no effect on respiratory volume, respiratory rate, blood pressure, or heart rate in anesthetized dogs. The ED50 for inhibition of skeletal muscle contractions was 4.5 mg/kg in anesthetized dogs. In anesthetized sheep, the ED50 for skeletal muscle relaxation was 3.2 mg/kg under methoxyflurane anesthesia and 1.7 mg/kg under pentobarbital anesthesia. Unanesthetized sheep administered doses up to 30 mg/kg iv evidenced no dose-related cardiovascular effects. Respiratory volume decreased and respiratory rate increased, with the net result that the respiratory minute volume was not affected by dantrolene sodium. The results indicate that dantrolene sodium has no effect on the cardiovascular or respiratory systems that would preclude its use intravenously in acute conditions where direct relaxation of skeletal muscle is required, as in the management of malignant hyperthermia.", "contents": "Effects of intravenous dantrolene sodium on respiratory and cardiovascular functions. Dantrolene sodium, a peripherally acting skeletal muscle relaxant, at doses up to 30 mg/kg iv had no effect on respiratory volume, respiratory rate, blood pressure, or heart rate in anesthetized dogs. The ED50 for inhibition of skeletal muscle contractions was 4.5 mg/kg in anesthetized dogs. In anesthetized sheep, the ED50 for skeletal muscle relaxation was 3.2 mg/kg under methoxyflurane anesthesia and 1.7 mg/kg under pentobarbital anesthesia. Unanesthetized sheep administered doses up to 30 mg/kg iv evidenced no dose-related cardiovascular effects. Respiratory volume decreased and respiratory rate increased, with the net result that the respiratory minute volume was not affected by dantrolene sodium. The results indicate that dantrolene sodium has no effect on the cardiovascular or respiratory systems that would preclude its use intravenously in acute conditions where direct relaxation of skeletal muscle is required, as in the management of malignant hyperthermia.", "PMID": 966155}
{"id": "pubmed23n0032_6606", "title": "Spectrophotometric study of complex formation between oxovanadium (IV) and antiamebic drugs.", "content": "Complex formation between oxovanadium(IV) and the antiamebic drugs 5, 7-dibromo-8-quinolinol and 5, 7-dichloro-8-quinolinol was studied in the pH 1.5-2.0 range, using ethanol, dioxane-water, and dimethylformamide as solvents. The composition of the formed complexes was determined by more than one procedure. In ethanol and dioxane-water, the 1:1 and 1:2 complexes were formed; in dimethylformamide, the 1:1, 1:2 and 1:3 complexes were formed. The stability constants were computed using two procedures: the molar ratio method and the extrapolation method. The reproducibility or results in satisfactory.", "contents": "Spectrophotometric study of complex formation between oxovanadium (IV) and antiamebic drugs. Complex formation between oxovanadium(IV) and the antiamebic drugs 5, 7-dibromo-8-quinolinol and 5, 7-dichloro-8-quinolinol was studied in the pH 1.5-2.0 range, using ethanol, dioxane-water, and dimethylformamide as solvents. The composition of the formed complexes was determined by more than one procedure. In ethanol and dioxane-water, the 1:1 and 1:2 complexes were formed; in dimethylformamide, the 1:1, 1:2 and 1:3 complexes were formed. The stability constants were computed using two procedures: the molar ratio method and the extrapolation method. The reproducibility or results in satisfactory.", "PMID": 966156}
{"id": "pubmed23n0032_6607", "title": "Hypocholesterolemic agents V: Inhibition of beta-hydroxy-beta-methylglutaryl coenzyme A reductase by substituted 4-biphenylylalkyl carboxylic acids and methyl esters.", "content": "Eleven substituted 4-biphenylylalkyl carboxylic acids and three methyl esters were synthesized and assayed for inhibition of rat liver beta-hydroxy-beta-methylglutaryl coenzyme A reductase. Five of the acids were analogs, resulting from various isosteric replacements of the carbonyl and ether oxygens of the previously described reversible inhibitor 1-(4-biphenylyl)pentyl hydrogen succinate. No significant change in activity was noted, except upon introduction of an amide linkage where a decrease in inhibition was found. Six carboxylic acids and three methyl esters, all containing the 4-biphenylyl radical but lacking the n-butyl side chain found in 1-(4-biphenylyl)pentyl hydrogen succinate, also were inhibitors of the reductase.", "contents": "Hypocholesterolemic agents V: Inhibition of beta-hydroxy-beta-methylglutaryl coenzyme A reductase by substituted 4-biphenylylalkyl carboxylic acids and methyl esters. Eleven substituted 4-biphenylylalkyl carboxylic acids and three methyl esters were synthesized and assayed for inhibition of rat liver beta-hydroxy-beta-methylglutaryl coenzyme A reductase. Five of the acids were analogs, resulting from various isosteric replacements of the carbonyl and ether oxygens of the previously described reversible inhibitor 1-(4-biphenylyl)pentyl hydrogen succinate. No significant change in activity was noted, except upon introduction of an amide linkage where a decrease in inhibition was found. Six carboxylic acids and three methyl esters, all containing the 4-biphenylyl radical but lacking the n-butyl side chain found in 1-(4-biphenylyl)pentyl hydrogen succinate, also were inhibitors of the reductase.", "PMID": 966157}
{"id": "pubmed23n0032_6608", "title": "Physical characterization and activity in vivo of polymorphic forms of 7-chloro-5, 11-dihydrodibenz[b, e][1, 4]oxazepine-5-carboxamide, a potential tricyclic antidepressant.", "content": "The biological availability in dogs and humans of 7-chloro-5, 11-dihydrodibenz[b,e][1,4]oxazepine-5-carboxamide, a potential antidepressant drug, was increased when the compound was administered in capsule formulations as micronized drug coated with 1% sodium lauryl sulfate or as a lyophilate with poloxamer 407. This increase with these two formulations had been predicted by dissolution tests in vitro. The lyophilized combination with poloxamer 407 was more soluble in 0.1 N HCl than was the untreated compound. Characterization of the lyophilate by differential thermal analysis, X-ray diffraction, and IR spectroscopy indicated that the increase in solubility was attributable to the formation of a polymorphic from. a polymorph of the compound designated form 8, was prepared. The solubility and dissolution characteristics of the two polymorphic forms, A and B, as well as of the lyophilized combination with poloxamer 407, were determined.", "contents": "Physical characterization and activity in vivo of polymorphic forms of 7-chloro-5, 11-dihydrodibenz[b, e][1, 4]oxazepine-5-carboxamide, a potential tricyclic antidepressant. The biological availability in dogs and humans of 7-chloro-5, 11-dihydrodibenz[b,e][1,4]oxazepine-5-carboxamide, a potential antidepressant drug, was increased when the compound was administered in capsule formulations as micronized drug coated with 1% sodium lauryl sulfate or as a lyophilate with poloxamer 407. This increase with these two formulations had been predicted by dissolution tests in vitro. The lyophilized combination with poloxamer 407 was more soluble in 0.1 N HCl than was the untreated compound. Characterization of the lyophilate by differential thermal analysis, X-ray diffraction, and IR spectroscopy indicated that the increase in solubility was attributable to the formation of a polymorphic from. a polymorph of the compound designated form 8, was prepared. The solubility and dissolution characteristics of the two polymorphic forms, A and B, as well as of the lyophilized combination with poloxamer 407, were determined.", "PMID": 966158}
{"id": "pubmed23n0032_6609", "title": "Epimeric 5-hydroxy-5-phenyl-1-azabicyclo [5.4.0] undecanes.", "content": "The development of order during the aging of aluminum hydroxide gel prepared by the reaction of aluminum chloride and ammonium hydroxide to a final pH of 7.0 can be monitored by differential thermal analysis. The loss fo acid reactivity upon aging is accompanied by an increase in the temperature and intensity of the dehydroxylation endotherm and an accompanying decrease in the intensity of the water of hydration endotherm. With continued aging, the thermogram develops the characteristics of a crystalline aluminum hydroxide.", "contents": "Epimeric 5-hydroxy-5-phenyl-1-azabicyclo [5.4.0] undecanes. The development of order during the aging of aluminum hydroxide gel prepared by the reaction of aluminum chloride and ammonium hydroxide to a final pH of 7.0 can be monitored by differential thermal analysis. The loss fo acid reactivity upon aging is accompanied by an increase in the temperature and intensity of the dehydroxylation endotherm and an accompanying decrease in the intensity of the water of hydration endotherm. With continued aging, the thermogram develops the characteristics of a crystalline aluminum hydroxide.", "PMID": 966160}
{"id": "pubmed23n0032_6610", "title": "Differential thermal analysis of aluminum hydroxide gel.", "content": "The development of order during the aging of aluminum hydroxide gel prepared by the reaction of aluminum chloride and ammonium hydroxide to a final pH of 7.0 can be monitored by differential thermal analysis. The loss of acid reactivity upon aging is accompanied by an increase in the temperature and intensity of the dehydroxylation endotherm and an accompanying decrease in the intensity of the water of hydration endotherm. With continued aging, the thermogram develops the characteristics of a crystalline aluminum hydroxide.", "contents": "Differential thermal analysis of aluminum hydroxide gel. The development of order during the aging of aluminum hydroxide gel prepared by the reaction of aluminum chloride and ammonium hydroxide to a final pH of 7.0 can be monitored by differential thermal analysis. The loss of acid reactivity upon aging is accompanied by an increase in the temperature and intensity of the dehydroxylation endotherm and an accompanying decrease in the intensity of the water of hydration endotherm. With continued aging, the thermogram develops the characteristics of a crystalline aluminum hydroxide.", "PMID": 966161}
{"id": "pubmed23n0032_6611", "title": "Extraction and GLC analysis of pyrithyldione in highly putrified human postmortem samples.", "content": "Pyrithyldione was isolated from highly putrified human brain and small intestine samples by ether extraction followed by alumina column cleanup. The total yield was 90+/- 5%. No derivatization was necessary prior to GLC analysis.", "contents": "Extraction and GLC analysis of pyrithyldione in highly putrified human postmortem samples. Pyrithyldione was isolated from highly putrified human brain and small intestine samples by ether extraction followed by alumina column cleanup. The total yield was 90+/- 5%. No derivatization was necessary prior to GLC analysis.", "PMID": 966162}
{"id": "pubmed23n0032_6612", "title": "Pharmacokinetics of skin penetration.", "content": "A model for estimating in vivo skin permeability coefficients is presented. Explicit expressions are derived for the permeability coefficient in terms of excretion rates and tissue absorption. The excretion rate has a linear asymptotic limit from which the permeability coefficient can be determined. The usefulness of the model is demonstrated with existing literature data.", "contents": "Pharmacokinetics of skin penetration. A model for estimating in vivo skin permeability coefficients is presented. Explicit expressions are derived for the permeability coefficient in terms of excretion rates and tissue absorption. The excretion rate has a linear asymptotic limit from which the permeability coefficient can be determined. The usefulness of the model is demonstrated with existing literature data.", "PMID": 966163}
{"id": "pubmed23n0032_6613", "title": "Tumor inhibitory agents from Vauquelinia corymbosa (Rosaceae).", "content": "The chloroform extract of Vauquelinia corymbosa Correa has shown activity against the P-388 lymphocytic leukemia test system. The constituents responsible for this activity were identified as uvaol, ursolic acid, and betulinic acid. Their identity was proven by melting point; mixed melting point; elemental analysis; IR, PMR, and mass spectra; and preparation of derivatives.", "contents": "Tumor inhibitory agents from Vauquelinia corymbosa (Rosaceae). The chloroform extract of Vauquelinia corymbosa Correa has shown activity against the P-388 lymphocytic leukemia test system. The constituents responsible for this activity were identified as uvaol, ursolic acid, and betulinic acid. Their identity was proven by melting point; mixed melting point; elemental analysis; IR, PMR, and mass spectra; and preparation of derivatives.", "PMID": 966165}
{"id": "pubmed23n0032_6614", "title": "Stimulation of the rabbit hypothalamus: caudal projections to respiratory and cardiovascular centres.", "content": "1. Electrical stimulation of a small area of the hypothalamus, lying about 2 mm dorsal to the lateral margin of the mammillary bodies, evoked a characteristic complex of effects in the anaesthetized rabbit. 2. The observed effects included apneusis followed by tachypnoea, increased arterial blood pressure, and vagally mediated bradycardia. Exophthalmost and pupillodilatation was commonly seen. The ears were erected and the vibrissae drawn forward. Somatic effects were weaker, but often included increased extensor tone, especially in the forelimbs. 3. The intensity of these effects was dependent upon the frequency of stimuli within the trains. Effects were weak or absent below 20 shocks/sec, and were near-maximal above 60 shocks/sec, when employing constant-current rectangular wave stimuli of 100-300 muA, 1 msec pulse duration and 5-9 sec train duration. 4. The effects were not dependent upon connexions with the cerebral cortex, but were mediated by pathways that projected ipsilaterally from each side of the hypothalamus through the mesencephalon. 5. The evidence suggests that this region of the rabbit hypothalamus is partly homologous with that hypothalamic area associated with the defence reaction in cat. However, the over-all responses to stimulation in the anaesthetized rabbit differed considerably from those described in the anaesthetized carnivore, suggesting that their integrated physiological responses to emotional threat are dissimilar.", "contents": "Stimulation of the rabbit hypothalamus: caudal projections to respiratory and cardiovascular centres. 1. Electrical stimulation of a small area of the hypothalamus, lying about 2 mm dorsal to the lateral margin of the mammillary bodies, evoked a characteristic complex of effects in the anaesthetized rabbit. 2. The observed effects included apneusis followed by tachypnoea, increased arterial blood pressure, and vagally mediated bradycardia. Exophthalmost and pupillodilatation was commonly seen. The ears were erected and the vibrissae drawn forward. Somatic effects were weaker, but often included increased extensor tone, especially in the forelimbs. 3. The intensity of these effects was dependent upon the frequency of stimuli within the trains. Effects were weak or absent below 20 shocks/sec, and were near-maximal above 60 shocks/sec, when employing constant-current rectangular wave stimuli of 100-300 muA, 1 msec pulse duration and 5-9 sec train duration. 4. The effects were not dependent upon connexions with the cerebral cortex, but were mediated by pathways that projected ipsilaterally from each side of the hypothalamus through the mesencephalon. 5. The evidence suggests that this region of the rabbit hypothalamus is partly homologous with that hypothalamic area associated with the defence reaction in cat. However, the over-all responses to stimulation in the anaesthetized rabbit differed considerably from those described in the anaesthetized carnivore, suggesting that their integrated physiological responses to emotional threat are dissimilar.", "PMID": 966173}
{"id": "pubmed23n0032_6615", "title": "Calcium exchange in vascular smooth muscle, action of noradrenaline and lanthanum.", "content": "1. The Na, K, Ca and Mg content and the 45Ca uptake and loss were determined in rat aortae incubated in physiological solution or in solution containing LaCl3 instead of CaCl2. 2. Aortae washed in La-solution contained less Ca and Na than controls in physiological solution, the K content was not modified and the Mg content was slightly decreased. 3. In 50 mM-La solution the 45Ca diffusion space was intermediate between the values found for the [14C]sorbitol space and the [14C]inulin space, indicating that there was no Ca entry within the cell nor Ca binding at superficial sites. 45Ca loss from the tissue was directly related to the La concentration. 4. Noradrenaline increased the rate of uptake of 45Ca into the Ca fraction resistant to displacement by La. This increase was dose dependent, a response of 50% of the maximum being produced by 2 x 10(-8) noradrenaline as for the contraction. In the presence of phentolamine, the dose-effect curves for the action of noradrenaline on 45Ca uptake were displaced in a manner characteristic of competitive antagonism. The rhoA2 for phentolamine was 7-8. 5. In physiological solution, the rate of loss of 45Ca, from the Ca fraction resistant to displacement by La, was increased by noradrenaline the ED50 was 2 x 10(-8) M, and the effect was abolished by phentolamine. 6. In view of the similarity of phentolamine rhoA2 estimated by measuring noradrenaline sensitive 45Ca uptake or noradrenaline evoked contraction, it is likely that the activation of alpha-adrenergic receptors is responsible for both effects.", "contents": "Calcium exchange in vascular smooth muscle, action of noradrenaline and lanthanum. 1. The Na, K, Ca and Mg content and the 45Ca uptake and loss were determined in rat aortae incubated in physiological solution or in solution containing LaCl3 instead of CaCl2. 2. Aortae washed in La-solution contained less Ca and Na than controls in physiological solution, the K content was not modified and the Mg content was slightly decreased. 3. In 50 mM-La solution the 45Ca diffusion space was intermediate between the values found for the [14C]sorbitol space and the [14C]inulin space, indicating that there was no Ca entry within the cell nor Ca binding at superficial sites. 45Ca loss from the tissue was directly related to the La concentration. 4. Noradrenaline increased the rate of uptake of 45Ca into the Ca fraction resistant to displacement by La. This increase was dose dependent, a response of 50% of the maximum being produced by 2 x 10(-8) noradrenaline as for the contraction. In the presence of phentolamine, the dose-effect curves for the action of noradrenaline on 45Ca uptake were displaced in a manner characteristic of competitive antagonism. The rhoA2 for phentolamine was 7-8. 5. In physiological solution, the rate of loss of 45Ca, from the Ca fraction resistant to displacement by La, was increased by noradrenaline the ED50 was 2 x 10(-8) M, and the effect was abolished by phentolamine. 6. In view of the similarity of phentolamine rhoA2 estimated by measuring noradrenaline sensitive 45Ca uptake or noradrenaline evoked contraction, it is likely that the activation of alpha-adrenergic receptors is responsible for both effects.", "PMID": 966174}
{"id": "pubmed23n0032_6616", "title": "Influence of amygdala stimulation on the activity of identified tuberoinfundibular neurones in the rat hypothalamus.", "content": "1. Extracellular action potentials were recorded from 1246 neurones in the mediobasal hypothalamus of pentobarbitone or urethane anaesthetized male rats. Antidromic invasion from the surface of the median eminence identified 165 cells, located in the arcuate and ventromedial nuclei and the periventricular area, as tuberoinfundibular neurones. The majority (65%) of these cells displayed no spontaneous activity. 2. Latencies for antidromic invasion from median eminence ranged from 0-5 to 14-0 msec (mean 4-3 +/- 2-9 msec, S.D.). Conduction velocities for axons of tuberoinfundibular neurones were under 1-0 m/sec, and were slowest (under 0-2 m/sec) for those tuberoinfundibular neurones located in the arcuate nucleus. 3. Single 1 HZ stimulation of amygdala evoked short latency (mean 18-8 +/- 7-0 msec; n = 30) excitation of tuberoinfundibular neurones in the ventromedial nucleus. Stria terminalis stimulation evoked similar responses at a shorter latency (mean 10-2 +/- 3-5 msec; n = 12) from other ventromedial tuberoinfundibular neurones. Three of these neurones were also excited by amygdala stimulation at comparably longer latencies. In spontaneously active tuberoinfundibular cells, the initial excitation was followed by a decrease in excitability lasting 70-150 msec. Tuberoinfundibular neurones soldom followed orthodromic activation at frequencies beyond 30 HZ. 4. An initial decrease in activity at latencies of 18-40 msec (mean 29-2 +/- 10-2 msec) characterized the amygdala evoked responses from nine tuberoinfundibular neurones. A similar response from one other tuberoinfundibular neurone followed stria terminalis stimulation at a latency of 11 msec. Most of these tuberoinfundibular neurones were located in the dorsal part of the ventromedial nucleus. 5. Two ventromedial tuberoinfundibular neurones also displayed antidromic invasion from the amygdala; interaction studies suggested an axon collateral pathway that originated close to the origin of the axon. 6. Tuberoinfundibular neurones unresponsive to amygdala stimulation were usually located in the arcuate nucleus or periventricular area. 7. These results provide electrophysiological evidence for a direct influence of the amygdala on the activity of tuberoinfundibular neurones in the ventromedial hypothalamic nucleus. There are also data to indicate that some ventromedial tuberoinfundibular neurones have axon collaterals that return to the amygdala. These reciprocal connexions between the amygdala and ventromedial tuberoinfundibular neurones may indicate neural circuits important for extrahypothalamic modulation of adenohypophyseal secretion.", "contents": "Influence of amygdala stimulation on the activity of identified tuberoinfundibular neurones in the rat hypothalamus. 1. Extracellular action potentials were recorded from 1246 neurones in the mediobasal hypothalamus of pentobarbitone or urethane anaesthetized male rats. Antidromic invasion from the surface of the median eminence identified 165 cells, located in the arcuate and ventromedial nuclei and the periventricular area, as tuberoinfundibular neurones. The majority (65%) of these cells displayed no spontaneous activity. 2. Latencies for antidromic invasion from median eminence ranged from 0-5 to 14-0 msec (mean 4-3 +/- 2-9 msec, S.D.). Conduction velocities for axons of tuberoinfundibular neurones were under 1-0 m/sec, and were slowest (under 0-2 m/sec) for those tuberoinfundibular neurones located in the arcuate nucleus. 3. Single 1 HZ stimulation of amygdala evoked short latency (mean 18-8 +/- 7-0 msec; n = 30) excitation of tuberoinfundibular neurones in the ventromedial nucleus. Stria terminalis stimulation evoked similar responses at a shorter latency (mean 10-2 +/- 3-5 msec; n = 12) from other ventromedial tuberoinfundibular neurones. Three of these neurones were also excited by amygdala stimulation at comparably longer latencies. In spontaneously active tuberoinfundibular cells, the initial excitation was followed by a decrease in excitability lasting 70-150 msec. Tuberoinfundibular neurones soldom followed orthodromic activation at frequencies beyond 30 HZ. 4. An initial decrease in activity at latencies of 18-40 msec (mean 29-2 +/- 10-2 msec) characterized the amygdala evoked responses from nine tuberoinfundibular neurones. A similar response from one other tuberoinfundibular neurone followed stria terminalis stimulation at a latency of 11 msec. Most of these tuberoinfundibular neurones were located in the dorsal part of the ventromedial nucleus. 5. Two ventromedial tuberoinfundibular neurones also displayed antidromic invasion from the amygdala; interaction studies suggested an axon collateral pathway that originated close to the origin of the axon. 6. Tuberoinfundibular neurones unresponsive to amygdala stimulation were usually located in the arcuate nucleus or periventricular area. 7. These results provide electrophysiological evidence for a direct influence of the amygdala on the activity of tuberoinfundibular neurones in the ventromedial hypothalamic nucleus. There are also data to indicate that some ventromedial tuberoinfundibular neurones have axon collaterals that return to the amygdala. These reciprocal connexions between the amygdala and ventromedial tuberoinfundibular neurones may indicate neural circuits important for extrahypothalamic modulation of adenohypophyseal secretion.", "PMID": 966175}
{"id": "pubmed23n0032_6617", "title": "The effect of temperature on the reaction of carbon monoxide with oxygenated haemoglobin.", "content": "1. The rate at which carbon monoxide displaces oxygen from its combination with haemoglobin in solution, has been measured spectrophotometrically, using a rapid-mixing stopped-flow technique. 2. In the presence of carbon dioxide, the reaction proceeds by a unimolecular dissociation, with a rate constant r. 3. The relationship of the reciprocal of r to the ratio PO2/PCO is non-linear, and a different curve is obtained at each carbon monoxide concentration. 4. From measurements of the rate constant at temperatures between 5 and 35 degrees C, it is concluded that the non-linearity is due to the formation at a finite rate of an intermediate complex, CO2Hb4O6, by the reaction of carbon dioxide with Hb4O6.", "contents": "The effect of temperature on the reaction of carbon monoxide with oxygenated haemoglobin. 1. The rate at which carbon monoxide displaces oxygen from its combination with haemoglobin in solution, has been measured spectrophotometrically, using a rapid-mixing stopped-flow technique. 2. In the presence of carbon dioxide, the reaction proceeds by a unimolecular dissociation, with a rate constant r. 3. The relationship of the reciprocal of r to the ratio PO2/PCO is non-linear, and a different curve is obtained at each carbon monoxide concentration. 4. From measurements of the rate constant at temperatures between 5 and 35 degrees C, it is concluded that the non-linearity is due to the formation at a finite rate of an intermediate complex, CO2Hb4O6, by the reaction of carbon dioxide with Hb4O6.", "PMID": 966176}
{"id": "pubmed23n0032_6618", "title": "Energetics of isovolumic contractions of the isolated rabbit heart.", "content": "1. In the isolated beating rabbit heart the heat production (measured by Dewar-flask calorimetry) and oxygen consumption (measured polarographically) increased in a similar way to force development (assessed as the time integral of left ventricular developed pressure) when the diastolic size of the heart was increased. 2. The energy expenditure of the heart consists of an element which is independent of the force developed and another which varies with the force developed. 3. The calorific equivalent of oxygen in the beating heart when it was provided with pyruvate as a substrate was found to be 20-48 mJ/mul: O2 at 25 degrees C. 4. The anaerobic metabolism was well below 5% of the total energy liberation and was constant at all levels of mechanical activity.", "contents": "Energetics of isovolumic contractions of the isolated rabbit heart. 1. In the isolated beating rabbit heart the heat production (measured by Dewar-flask calorimetry) and oxygen consumption (measured polarographically) increased in a similar way to force development (assessed as the time integral of left ventricular developed pressure) when the diastolic size of the heart was increased. 2. The energy expenditure of the heart consists of an element which is independent of the force developed and another which varies with the force developed. 3. The calorific equivalent of oxygen in the beating heart when it was provided with pyruvate as a substrate was found to be 20-48 mJ/mul: O2 at 25 degrees C. 4. The anaerobic metabolism was well below 5% of the total energy liberation and was constant at all levels of mechanical activity.", "PMID": 966177}
{"id": "pubmed23n0032_6619", "title": "The effects of potassium and temperature on the pace-maker current, iK2, in Purkinje fibres.", "content": "1. The reversal potential for the pace-maker K current, iK2, was measured in sheep cardiac Purkinje fibres at extracellular K concentrations, [K]O, between 2-7 and 8 mM. The reversal potentials were found to be significantly more negative than the values predicted using the Nernst equation for any reasonable value of intracellular K+ concentration or activity. 2. It is suggested that this discrepancy may be explained by postulating that the extracellular K+ concentration [K]e in the cleft spaces between cells is smaller than [K]o as a result of ion pumping and restricted diffusion from the bulk extracellular medium. 3. In conformity with this hypothesis, it was shown that the value of [K]e may be further reduced by hyperpolarizing pulses, presumably as a consequence of K+ depletion during the passage of inward current. 4. The influence of temperature on the kinetics of the gating mechanism, s, controlling iK2 was investigated. The Q10 for the time constant, pis, of current change following voltage clamp steps was found to be about 17. This corresponds to an apparent activation enthalpy of 50 kcal/mole. 5. The Q10 of the maximum amplitude iotaK2, was found to 1-3. 6. The activation curve, s infinity (Em), spread slightly to more negative potentials by cooling from 37 to 30 degrees C and the curve became less steep. 7. There is a large decrease in the inward background current on cooling, as estimated by measuring the net membrane current when iK is presumed to be zero, i.e. at the reversal potential for iK2.", "contents": "The effects of potassium and temperature on the pace-maker current, iK2, in Purkinje fibres. 1. The reversal potential for the pace-maker K current, iK2, was measured in sheep cardiac Purkinje fibres at extracellular K concentrations, [K]O, between 2-7 and 8 mM. The reversal potentials were found to be significantly more negative than the values predicted using the Nernst equation for any reasonable value of intracellular K+ concentration or activity. 2. It is suggested that this discrepancy may be explained by postulating that the extracellular K+ concentration [K]e in the cleft spaces between cells is smaller than [K]o as a result of ion pumping and restricted diffusion from the bulk extracellular medium. 3. In conformity with this hypothesis, it was shown that the value of [K]e may be further reduced by hyperpolarizing pulses, presumably as a consequence of K+ depletion during the passage of inward current. 4. The influence of temperature on the kinetics of the gating mechanism, s, controlling iK2 was investigated. The Q10 for the time constant, pis, of current change following voltage clamp steps was found to be about 17. This corresponds to an apparent activation enthalpy of 50 kcal/mole. 5. The Q10 of the maximum amplitude iotaK2, was found to 1-3. 6. The activation curve, s infinity (Em), spread slightly to more negative potentials by cooling from 37 to 30 degrees C and the curve became less steep. 7. There is a large decrease in the inward background current on cooling, as estimated by measuring the net membrane current when iK is presumed to be zero, i.e. at the reversal potential for iK2.", "PMID": 966178}
{"id": "pubmed23n0032_6620", "title": "An analysis of the actions of low concentrations of ouabain on membrane currents in Purkinje fibres.", "content": "1. The influence of low concentrations (5 X 10(-8) to 5 X 10(-7) M) of ouabain on the K gradient in sheep cardiac Purkinje fibres was observed by measuring changes in the reversal potential for a K specific current iK2, and by measuring total steady-state current-voltage relations. 2. Provided that the bathing solution K concentration, [K]o  was not too low, these doses of ouabain were often observed to increase the K gradient, i.e. the reversal potential was shifted in a negative direction. 3. The change in the reversal potential and in the current-voltage relation could be mimicked by reducing the value of [K]o in the absence of ouabain. It is therefore suggested that ouabain may stimulate the Na+-K+ exchange pump and so reduce the K concentration, [K]e, in the clefts of the preparation. 4. At sufficiently low values of [K]o, a dose of ouabain that was stimulatory may become inhibitory. The reversal potential for iK2 then shifts in a positive direction. 5. During either stimulation or inhibition, the speed of change of reversal potential is consistent with a change in [K]e, which may change fairly rapidly. It is not possible to account for the results solely by changes in intracellular concentration, [K]i. 6. Low concentrations of ouabain were found to have no effect on the activation curve, s infinity (Em), controlling iK2. It is concluded that the changes in iK2 are solely attributable to changes in reversal potential. 7. Since net stimulation of the Na+-K+ exchange pump was observed to occur at doses of ouabain that exert a strong positive inotropic action on Purkinje fibres (Blood, 1975), it is not likely that the inotropic action is causally related to net pump inhibition.", "contents": "An analysis of the actions of low concentrations of ouabain on membrane currents in Purkinje fibres. 1. The influence of low concentrations (5 X 10(-8) to 5 X 10(-7) M) of ouabain on the K gradient in sheep cardiac Purkinje fibres was observed by measuring changes in the reversal potential for a K specific current iK2, and by measuring total steady-state current-voltage relations. 2. Provided that the bathing solution K concentration, [K]o  was not too low, these doses of ouabain were often observed to increase the K gradient, i.e. the reversal potential was shifted in a negative direction. 3. The change in the reversal potential and in the current-voltage relation could be mimicked by reducing the value of [K]o in the absence of ouabain. It is therefore suggested that ouabain may stimulate the Na+-K+ exchange pump and so reduce the K concentration, [K]e, in the clefts of the preparation. 4. At sufficiently low values of [K]o, a dose of ouabain that was stimulatory may become inhibitory. The reversal potential for iK2 then shifts in a positive direction. 5. During either stimulation or inhibition, the speed of change of reversal potential is consistent with a change in [K]e, which may change fairly rapidly. It is not possible to account for the results solely by changes in intracellular concentration, [K]i. 6. Low concentrations of ouabain were found to have no effect on the activation curve, s infinity (Em), controlling iK2. It is concluded that the changes in iK2 are solely attributable to changes in reversal potential. 7. Since net stimulation of the Na+-K+ exchange pump was observed to occur at doses of ouabain that exert a strong positive inotropic action on Purkinje fibres (Blood, 1975), it is not likely that the inotropic action is causally related to net pump inhibition.", "PMID": 966179}
{"id": "pubmed23n0032_6621", "title": "[Digestion and absorption of dietary triglycerides].", "content": "This review is an attempt to put in order some facts obtained during twenty-five years of studying the digestion and absorption of dietary triglycerides. After a short history of this problem to show the progress of research, the active mechanism of pancreatic lipase on triglycerides is explained: this enzyme specifically hydrolyses fatty acids esterified with the primary hydroxyl groups of glycerol, forming 2-monoglycerides and free fatty acids. This fact is very important for the further process involving formation and composition of intraluminal micelles, along with absorption and de novo synthesis of triglycerides inside enterocytes. Several different points are disscussed: - Short or medium chain-length fatty acids are hydrolyzed more easily than long-chain ones; after their absorption, the former go to the liver by the portal circulation and the latter go to lymph. - An evaluation of the extent of digestive hydrolysis has been given by means of doubly-labelled triglycerides (glycerol and fatty acids), and by comparing isotope ratio values of lymph and dietary triglycerides. - The physico-chemical state that intraluminal fats are in is discussed; 2-monoglycerides and fatty acids released from triglyceride hydrolysis form macromolecular agregates with biliary salts and phospholipids, cholesterol, and dietary phospholipids (or their degradation products). These are termed mixed micelles and are absorbed by diffusion. - Many recent morphological studies carried out with the electron microscope are indicated. - Two metabolic pathways for triglyceride resynthesis in the mucosal cells have been established, one from free fatty acids and alpha-glycerophosphate, the other from 1 or 2-monoglycerides. Phosphatidic acids are involved in this resynthesis, but the role of other phospholipids is almost certainly not so narrowly limited. - The apparent digestibility of fats according to their fatty acid composition, and mainly according to the location of the fatty acids in the initial triglyceride molecule, is discussed. - The nature and importance of endogenous fatty acids in the digestive tract and lymph is discussed. - The lipid and fatty acid composition of lymph chylomicrons is given. - The importance of lymph lipid particles (average diameter 0,05 less than or equal mum) for transport of exogenous fatty acids is indicated. The biochemical mechanism involved in the digestion and absorption of dietary lipids are now well known, but some processes are still uncertain, particularly the physico-chemical state of intraluminal lipids, the role of enterocyte membranes, the synthesis and secretion of digestive enzymes, and the role of intestinal flora.", "contents": "[Digestion and absorption of dietary triglycerides]. This review is an attempt to put in order some facts obtained during twenty-five years of studying the digestion and absorption of dietary triglycerides. After a short history of this problem to show the progress of research, the active mechanism of pancreatic lipase on triglycerides is explained: this enzyme specifically hydrolyses fatty acids esterified with the primary hydroxyl groups of glycerol, forming 2-monoglycerides and free fatty acids. This fact is very important for the further process involving formation and composition of intraluminal micelles, along with absorption and de novo synthesis of triglycerides inside enterocytes. Several different points are disscussed: - Short or medium chain-length fatty acids are hydrolyzed more easily than long-chain ones; after their absorption, the former go to the liver by the portal circulation and the latter go to lymph. - An evaluation of the extent of digestive hydrolysis has been given by means of doubly-labelled triglycerides (glycerol and fatty acids), and by comparing isotope ratio values of lymph and dietary triglycerides. - The physico-chemical state that intraluminal fats are in is discussed; 2-monoglycerides and fatty acids released from triglyceride hydrolysis form macromolecular agregates with biliary salts and phospholipids, cholesterol, and dietary phospholipids (or their degradation products). These are termed mixed micelles and are absorbed by diffusion. - Many recent morphological studies carried out with the electron microscope are indicated. - Two metabolic pathways for triglyceride resynthesis in the mucosal cells have been established, one from free fatty acids and alpha-glycerophosphate, the other from 1 or 2-monoglycerides. Phosphatidic acids are involved in this resynthesis, but the role of other phospholipids is almost certainly not so narrowly limited. - The apparent digestibility of fats according to their fatty acid composition, and mainly according to the location of the fatty acids in the initial triglyceride molecule, is discussed. - The nature and importance of endogenous fatty acids in the digestive tract and lymph is discussed. - The lipid and fatty acid composition of lymph chylomicrons is given. - The importance of lymph lipid particles (average diameter 0,05 less than or equal mum) for transport of exogenous fatty acids is indicated. The biochemical mechanism involved in the digestion and absorption of dietary lipids are now well known, but some processes are still uncertain, particularly the physico-chemical state of intraluminal lipids, the role of enterocyte membranes, the synthesis and secretion of digestive enzymes, and the role of intestinal flora.", "PMID": 966180}
{"id": "pubmed23n0032_6622", "title": "[Hand blood flow at rest and during submaximal exercise in acute and chronic heat stress (author's transl)].", "content": "Three groups of subjects, Europeans without any heat acclimation (called EE), Europeans after 3 weeks of acclimatization in India (EI), and Indians in their natural environment (II) were studied during exposure to an ambient temperature of 33 degrees C. Hand blood flow (Q), rectal temperature (Tre), mean cutaneous temperature (Tsk) were simultaneously recorded at rest and during 2 periods of muscular exercise (0.44 and 0.7 Vo2 max) of 35 mn duration. The results showed (1) at rest, Q was very high in EE, quite low in both EI and II; (2) at the onset of exercise, a hand vasoconstriction was observed in all cases; (3) during exercise, there was a progressive increase of Q until 200% maximum above rest values; (4) at the end of exercise, Q was proportional to the intensity of the exercise for each group and inversely proportional to the duration of heat exposure, the highest Q was observed in EE, the lowest in II and an intermediate value for EI close to the latter). These differences in hand blood flow could not be explained by differences in deep and/or superficial temperatures between subjects. Thus, during chronic heat exposure, there is, especially for an exercising man, a progressive modification of heat transport in the body: that is, a reduction of skin perfusion and a greater Tre-Tsk difference which are both adaptative responses. The value of hand blood flow as an estimation of whole superficial circulation is discussed.", "contents": "[Hand blood flow at rest and during submaximal exercise in acute and chronic heat stress (author's transl)]. Three groups of subjects, Europeans without any heat acclimation (called EE), Europeans after 3 weeks of acclimatization in India (EI), and Indians in their natural environment (II) were studied during exposure to an ambient temperature of 33 degrees C. Hand blood flow (Q), rectal temperature (Tre), mean cutaneous temperature (Tsk) were simultaneously recorded at rest and during 2 periods of muscular exercise (0.44 and 0.7 Vo2 max) of 35 mn duration. The results showed (1) at rest, Q was very high in EE, quite low in both EI and II; (2) at the onset of exercise, a hand vasoconstriction was observed in all cases; (3) during exercise, there was a progressive increase of Q until 200% maximum above rest values; (4) at the end of exercise, Q was proportional to the intensity of the exercise for each group and inversely proportional to the duration of heat exposure, the highest Q was observed in EE, the lowest in II and an intermediate value for EI close to the latter). These differences in hand blood flow could not be explained by differences in deep and/or superficial temperatures between subjects. Thus, during chronic heat exposure, there is, especially for an exercising man, a progressive modification of heat transport in the body: that is, a reduction of skin perfusion and a greater Tre-Tsk difference which are both adaptative responses. The value of hand blood flow as an estimation of whole superficial circulation is discussed.", "PMID": 966181}
{"id": "pubmed23n0032_6623", "title": "Vocational training for general practice-the Spalding seminars. A technique of topic teaching for trainees.", "content": "A technique for topic teaching for vocational trainees is described which matches trainees to patients to illustrate different facets of the topic under discussion.", "contents": "Vocational training for general practice-the Spalding seminars. A technique of topic teaching for trainees. A technique for topic teaching for vocational trainees is described which matches trainees to patients to illustrate different facets of the topic under discussion.", "PMID": 966206}
{"id": "pubmed23n0032_6624", "title": "Music and handicapped children.", "content": "Handicapped children may gain considerably from being introduced systematically to musical sounds. The benefit comes not only from enjoyment, but also from promoting intellectual, emotional, and social development. Some children who are severely handicapped intellectually may have great musical talent, such as perfect pitch, and if this is systematically cultivated a key can sometimes be found unlocking the barriers to the child's progress. Some examples are described.Several years' experience of working as a music therapist with children both normal and handicapped have convinced me that the use of music as an aid to learning is worth further study and research.", "contents": "Music and handicapped children. Handicapped children may gain considerably from being introduced systematically to musical sounds. The benefit comes not only from enjoyment, but also from promoting intellectual, emotional, and social development. Some children who are severely handicapped intellectually may have great musical talent, such as perfect pitch, and if this is systematically cultivated a key can sometimes be found unlocking the barriers to the child's progress. Some examples are described.Several years' experience of working as a music therapist with children both normal and handicapped have convinced me that the use of music as an aid to learning is worth further study and research.", "PMID": 966207}
{"id": "pubmed23n0032_6625", "title": "Complications of \"slow-K\" therapy.", "content": "(1) The failure of ;Slow-K' tablets to disintegrate prevents rapid release but allows them to be trapped by their bulk in the intestine.(2) Two cases are reported. In the first the tablet was trapped in a caecal diverticulum and the patient developed an abcess. In the second, abdominal pain developed which subsided when ;Slow-K' was stopped. Later ;Slow-K' was again started and the patient developed dysphagia.(3) The possibility of abdominal complications with this treatment should be remembered.(4) Effervescent KC1 preparations may replace ;Slow-K' but KC1 supplementation may be necessary only in cardiac disease.", "contents": "Complications of \"slow-K\" therapy. (1) The failure of ;Slow-K' tablets to disintegrate prevents rapid release but allows them to be trapped by their bulk in the intestine.(2) Two cases are reported. In the first the tablet was trapped in a caecal diverticulum and the patient developed an abcess. In the second, abdominal pain developed which subsided when ;Slow-K' was stopped. Later ;Slow-K' was again started and the patient developed dysphagia.(3) The possibility of abdominal complications with this treatment should be remembered.(4) Effervescent KC1 preparations may replace ;Slow-K' but KC1 supplementation may be necessary only in cardiac disease.", "PMID": 966209}
{"id": "pubmed23n0032_6626", "title": "Consultation patterns in a general practice.", "content": "A study from the Oxford Community Health Project is reported on the effects on consultation rates in general practice of patients' age, sex, date of registration, address, and usual doctor. The impact on consultation rates of accessibility of surgeries and availability of doctors is examined when the variables are controlled, and the importance of these factors is discussed in relation to planning primary medical care.", "contents": "Consultation patterns in a general practice. A study from the Oxford Community Health Project is reported on the effects on consultation rates in general practice of patients' age, sex, date of registration, address, and usual doctor. The impact on consultation rates of accessibility of surgeries and availability of doctors is examined when the variables are controlled, and the importance of these factors is discussed in relation to planning primary medical care.", "PMID": 966210}
{"id": "pubmed23n0032_6627", "title": "Otitis externa in children.", "content": "The commonest cause of ear-ache in children is otitis externa and five new cases of otitis externa will be seen for every case of otitis media.ACUTE OTITIS EXTERNA AND OTITIS MEDIA ARE QUITE DIFFERENT AND DISTINCT CLINICAL ENTITIES IN CHILDREN: the former is a tender, dirty, pruritic ear, often recurring in children with simple febrile illnesses; the latter is more isolated than is realised, non-recurrent and usually accompanying upper respiratory catarrhal illness.The fleeting nature of otitis externa as seen in childhood is typical of clinical material in general practice that presents quite differently from that in hospital practice. The long aetiological lists quoted in all series of cases of otitis externa in adults do not apply to otitis externa as seen in children.", "contents": "Otitis externa in children. The commonest cause of ear-ache in children is otitis externa and five new cases of otitis externa will be seen for every case of otitis media.ACUTE OTITIS EXTERNA AND OTITIS MEDIA ARE QUITE DIFFERENT AND DISTINCT CLINICAL ENTITIES IN CHILDREN: the former is a tender, dirty, pruritic ear, often recurring in children with simple febrile illnesses; the latter is more isolated than is realised, non-recurrent and usually accompanying upper respiratory catarrhal illness.The fleeting nature of otitis externa as seen in childhood is typical of clinical material in general practice that presents quite differently from that in hospital practice. The long aetiological lists quoted in all series of cases of otitis externa in adults do not apply to otitis externa as seen in children.", "PMID": 966211}
{"id": "pubmed23n0032_6628", "title": "Illness in a summer holiday camp.", "content": "(1) The medical needs of patients staying at a holiday camp during the summer of 1974 have been analysed.(2) Medical advice was sought from a doctor about half as often as would have been expected from a comparable population at home, but the camp nurse saw three times as many patients as the doctor.(3) The clinical content of the consultation was analysed.(4) The outcome of the consultations and the role played by the nurse are discussed.", "contents": "Illness in a summer holiday camp. (1) The medical needs of patients staying at a holiday camp during the summer of 1974 have been analysed.(2) Medical advice was sought from a doctor about half as often as would have been expected from a comparable population at home, but the camp nurse saw three times as many patients as the doctor.(3) The clinical content of the consultation was analysed.(4) The outcome of the consultations and the role played by the nurse are discussed.", "PMID": 966212}
{"id": "pubmed23n0032_6629", "title": "Lead and multiple sclerosis.", "content": "It has been suggested that lead may be a cause of multiple sclerosis, but confirmation of this hypothesis is difficult to obtain as lead is so widely present both in the environment and in many human tissues.In this study 22 patients with multiple sclerosis were compared with 22 controls. Lead levels in the blood of the patients were not higher and urinary lead levels in the patients before and after a single dose of penicillamine did not provide evidence to support the hypothesis that lead may cause the disease.", "contents": "Lead and multiple sclerosis. It has been suggested that lead may be a cause of multiple sclerosis, but confirmation of this hypothesis is difficult to obtain as lead is so widely present both in the environment and in many human tissues.In this study 22 patients with multiple sclerosis were compared with 22 controls. Lead levels in the blood of the patients were not higher and urinary lead levels in the patients before and after a single dose of penicillamine did not provide evidence to support the hypothesis that lead may cause the disease.", "PMID": 966213}
{"id": "pubmed23n0032_6630", "title": "A difference in dry mass between the heads of X- and Y-bearing human spermatozoa.", "content": "An integrating microinterferometer was used to measure the dry mass of sperm heads. The dry mass was found to be proportional to DNA content, and thus provides a useful method of estimating sperm DNA content. Using this technique we have confirmed that human spermatozoa which show none and one quinacrine-fluorescent spot are X- and Y-bearing respectively. However, the measurements suggest that many of the spermatozoa with two quinacrine-fluorescent spots are not YY-bearing, as previously thought, but might be incompletely condensed Y-bearing spermatozoa.", "contents": "A difference in dry mass between the heads of X- and Y-bearing human spermatozoa. An integrating microinterferometer was used to measure the dry mass of sperm heads. The dry mass was found to be proportional to DNA content, and thus provides a useful method of estimating sperm DNA content. Using this technique we have confirmed that human spermatozoa which show none and one quinacrine-fluorescent spot are X- and Y-bearing respectively. However, the measurements suggest that many of the spermatozoa with two quinacrine-fluorescent spots are not YY-bearing, as previously thought, but might be incompletely condensed Y-bearing spermatozoa.", "PMID": 966214}
{"id": "pubmed23n0032_6631", "title": "Protein composition in the fluid of individual bovine follicles.", "content": "The proteins in follicular fluid from individual and pooled bovine follicles were studied by gel chromatography and quantitative immunoelectrophoresis. The mean protein concentration was 86-4% of serum; very large proteins were present in only low concentrations. A minimum of 40 individual proteins was distinguished in follicular fluid, and 15 of these proteins were quantitated. A correlation between molecular weight and follicular fluid: serum concentration ratio was found. Fluid from individual follicles differed only in the relative concentrations of small and large proteins. An exception to this was IgG which was occasionally, but never in healthy growing follicles, present in concetrations above 150% of serum. Healthy growing, preovulatory and atretic follicles had higher, and cystic follicles mostly lower, concentrations of small proteins than serum. The concentration of alpha2-macroglobulin in healthy growing follicles never exceeded 16% of serum. The concentration of large proteins in follicular fluid increased with increasing follicle size. Attempts to detect proteins specific to follicular fluid by immunizing rabbits with pooled follicular samples and the follicular fluid proteins not bound by anti-bovine antiserum resulted in production of antibodies against fibrinogen and its split products D+E only.", "contents": "Protein composition in the fluid of individual bovine follicles. The proteins in follicular fluid from individual and pooled bovine follicles were studied by gel chromatography and quantitative immunoelectrophoresis. The mean protein concentration was 86-4% of serum; very large proteins were present in only low concentrations. A minimum of 40 individual proteins was distinguished in follicular fluid, and 15 of these proteins were quantitated. A correlation between molecular weight and follicular fluid: serum concentration ratio was found. Fluid from individual follicles differed only in the relative concentrations of small and large proteins. An exception to this was IgG which was occasionally, but never in healthy growing follicles, present in concetrations above 150% of serum. Healthy growing, preovulatory and atretic follicles had higher, and cystic follicles mostly lower, concentrations of small proteins than serum. The concentration of alpha2-macroglobulin in healthy growing follicles never exceeded 16% of serum. The concentration of large proteins in follicular fluid increased with increasing follicle size. Attempts to detect proteins specific to follicular fluid by immunizing rabbits with pooled follicular samples and the follicular fluid proteins not bound by anti-bovine antiserum resulted in production of antibodies against fibrinogen and its split products D+E only.", "PMID": 966215}
{"id": "pubmed23n0032_6632", "title": "Effect of removal of epididymal secretions on fertilization in vitro of mouse eggs.", "content": "The removal by centrifugation of epididymal contents from mouse spermatozoa had no deleterious effect on fertilization in vitro, and, depending on the genotype of the gametes, was frequently associated with increased levels of fertility. Washing of the spermatozoa significantly improved the fertilization rate of F1 eggs with TO spermatozoa and of BALB/c eggs with BALB/c spermatozoa, but had no significant effect of F1 spermatozoa with F1 eggs.", "contents": "Effect of removal of epididymal secretions on fertilization in vitro of mouse eggs. The removal by centrifugation of epididymal contents from mouse spermatozoa had no deleterious effect on fertilization in vitro, and, depending on the genotype of the gametes, was frequently associated with increased levels of fertility. Washing of the spermatozoa significantly improved the fertilization rate of F1 eggs with TO spermatozoa and of BALB/c eggs with BALB/c spermatozoa, but had no significant effect of F1 spermatozoa with F1 eggs.", "PMID": 966216}
{"id": "pubmed23n0032_6633", "title": "Ultrastructural studies of frozen-thawed 8-cell mouse embryos.", "content": "Survival of frozen 8-cell mouse embryos transferred directly upon thawing to the uteri of Day 3 pseudopregnant foster mothers was significantly lower (26%) than the survival of unfrozen 8-cell embryos transferred immediately after collection (73%). When frozen-thawed 8-cell embryos were cultured for 20-24 hr before transfer survival was similar to that of unfrozen 8-cell embryos transferred after 20-24 hr inculture (65% and 73%, respectively). Ultrastructural examination of the frozen-thawed 8-cell embryos revealed no obvious damage to protoplasmic components.", "contents": "Ultrastructural studies of frozen-thawed 8-cell mouse embryos. Survival of frozen 8-cell mouse embryos transferred directly upon thawing to the uteri of Day 3 pseudopregnant foster mothers was significantly lower (26%) than the survival of unfrozen 8-cell embryos transferred immediately after collection (73%). When frozen-thawed 8-cell embryos were cultured for 20-24 hr before transfer survival was similar to that of unfrozen 8-cell embryos transferred after 20-24 hr inculture (65% and 73%, respectively). Ultrastructural examination of the frozen-thawed 8-cell embryos revealed no obvious damage to protoplasmic components.", "PMID": 966217}
{"id": "pubmed23n0032_6634", "title": "Uterine luminal proteins at the time of implantation in rats.", "content": "Changes in rat urerine luminal proteins were analysed during pregnancy and after ovariectomy on Day 3 of pregnancy plus daily administration of progesterone, when delay of implantation is imposed. Total protein levels reached a peak level on Day 5 of pregnancy, the day of implantation, in the intact but not the ovariectomized females. Qualitative analyses of protein-SDS complexes showed an increase in a protein of molecular weight of approximately 70.000 and others of higher molecular weight during pregnancy, but in ovariectomized females the proteins of higher molecular weight were virtually undetectable by Day 10. The entry of blastocysts into quiescence after ovariectomy appears to be an adaptation by the embryos to suboptimal uterine luminal conditions.", "contents": "Uterine luminal proteins at the time of implantation in rats. Changes in rat urerine luminal proteins were analysed during pregnancy and after ovariectomy on Day 3 of pregnancy plus daily administration of progesterone, when delay of implantation is imposed. Total protein levels reached a peak level on Day 5 of pregnancy, the day of implantation, in the intact but not the ovariectomized females. Qualitative analyses of protein-SDS complexes showed an increase in a protein of molecular weight of approximately 70.000 and others of higher molecular weight during pregnancy, but in ovariectomized females the proteins of higher molecular weight were virtually undetectable by Day 10. The entry of blastocysts into quiescence after ovariectomy appears to be an adaptation by the embryos to suboptimal uterine luminal conditions.", "PMID": 966218}
{"id": "pubmed23n0032_6635", "title": "The fertilizing capacity of epididymal spermatozoa in relation to age, body weight and the onset of sexual maturity in the golden hamster.", "content": "Experiments with prepubertal hamsters were undertaken to determine if the appearance of spermatozoa with fertilizing capacity in the cauda epididymidis is related to age and/or body weight and whether the development of sperm function coincides with complete maturation of the mating behaviour pattern. At Week 6 after birth, intrauterine insemination of comparable numbers of spermatozoa showed that fertilizing capacity was related to body weight and not to age or seminal vesicular fructose concentration. In contrast to the low fertilization rate produced by spermatozoa from males weighing 80 g at Week 6 after birth, spermatozoa from 80-g males at Week 7 showed normal fertilizing capacity. Only 50% of 7-week-old males weighing 100-110 g produced fertile matings whereas all matings were fertile with males weighing 135 g and with males weighing 100 to 110 g that had received 7 consecutive daily injections of 50 mug testosterone beginning at Week 6 after birth. Comparison of sperm numbers in the cauda epididymidis and ductus deferens after mating at Week 7 showed that males which produced sterile matings were unable to terminate intromission with normal ejaculation of spermatozoa.", "contents": "The fertilizing capacity of epididymal spermatozoa in relation to age, body weight and the onset of sexual maturity in the golden hamster. Experiments with prepubertal hamsters were undertaken to determine if the appearance of spermatozoa with fertilizing capacity in the cauda epididymidis is related to age and/or body weight and whether the development of sperm function coincides with complete maturation of the mating behaviour pattern. At Week 6 after birth, intrauterine insemination of comparable numbers of spermatozoa showed that fertilizing capacity was related to body weight and not to age or seminal vesicular fructose concentration. In contrast to the low fertilization rate produced by spermatozoa from males weighing 80 g at Week 6 after birth, spermatozoa from 80-g males at Week 7 showed normal fertilizing capacity. Only 50% of 7-week-old males weighing 100-110 g produced fertile matings whereas all matings were fertile with males weighing 135 g and with males weighing 100 to 110 g that had received 7 consecutive daily injections of 50 mug testosterone beginning at Week 6 after birth. Comparison of sperm numbers in the cauda epididymidis and ductus deferens after mating at Week 7 showed that males which produced sterile matings were unable to terminate intromission with normal ejaculation of spermatozoa.", "PMID": 966219}
{"id": "pubmed23n0032_6636", "title": "Proteins in human uterine fluid.", "content": "The proteins in saline washings from uteri of women at different stages of the menstrual cycle were examined by polacrylamide gel electrophoresis at pH 4-5 and pH 8-9, isoelectric focusing in polyacrylamide, and immunoelectrophoresis. The components present were mainly serum proteins but small amounts of uterine-specific proteins were also detected. Measurement of the activities of several glycosidases in uterine washings revealed that alpha-L-fucosidase, beta-N-acetylgalactosaminidase and beta-N-acetylglucosaminidase were elevated when compared with serum.", "contents": "Proteins in human uterine fluid. The proteins in saline washings from uteri of women at different stages of the menstrual cycle were examined by polacrylamide gel electrophoresis at pH 4-5 and pH 8-9, isoelectric focusing in polyacrylamide, and immunoelectrophoresis. The components present were mainly serum proteins but small amounts of uterine-specific proteins were also detected. Measurement of the activities of several glycosidases in uterine washings revealed that alpha-L-fucosidase, beta-N-acetylgalactosaminidase and beta-N-acetylglucosaminidase were elevated when compared with serum.", "PMID": 966220}
{"id": "pubmed23n0032_6637", "title": "Effects of RMI 12,936, a synthetic antiprogestational steroid, in the rat.", "content": "The antifertility effect of RMI 12,936 in the rat is shown to be reversible. Luteal hypertrophy followed administration to pregnant, but not to pregnant hysterectomized rats. Similar hypertrophy followed administration to pseudopregnant, immature gonadotrophin-treated, and immature hysterectomized and gonadotrophin-treated rats, but the ovarian sensitivity to gonadotrophins was reduced or unchanged, suggesting that the ovarian hypertrophy is due to a direct ovarian action of RMI 12,936. Progesterone production on Day 15 of pregnancy by the enlarged ovaries following RMI 12,936 administration on Day 8 was not significantly different from control levels, but there was a highly significantly reduction on Day 9. An isomer of RMI 12,936, Tsomer 201, presumed to be 7alpha-methyltestosterone, was  shown to have antifertility and uterotrophic activities in rats similar to those of RMI 12,936. Unlike RMI 12,936, however, Isomer 201 did show significant cross-reaction in the competitive protein-binding assay for progesterone and did not cause significant reduction in peripheral progesterone levels on Day 9 after administration on Day 8. From this and previous evidence it is concluded that RMI 12,936 inhibits progesterone synthesis, possibly by acting as an alternative substrate for ovarian delta(5)3-ketosteroid isomerase, and that the product is probably a competitive antagonist of progesterone at the receptor level.", "contents": "Effects of RMI 12,936, a synthetic antiprogestational steroid, in the rat. The antifertility effect of RMI 12,936 in the rat is shown to be reversible. Luteal hypertrophy followed administration to pregnant, but not to pregnant hysterectomized rats. Similar hypertrophy followed administration to pseudopregnant, immature gonadotrophin-treated, and immature hysterectomized and gonadotrophin-treated rats, but the ovarian sensitivity to gonadotrophins was reduced or unchanged, suggesting that the ovarian hypertrophy is due to a direct ovarian action of RMI 12,936. Progesterone production on Day 15 of pregnancy by the enlarged ovaries following RMI 12,936 administration on Day 8 was not significantly different from control levels, but there was a highly significantly reduction on Day 9. An isomer of RMI 12,936, Tsomer 201, presumed to be 7alpha-methyltestosterone, was  shown to have antifertility and uterotrophic activities in rats similar to those of RMI 12,936. Unlike RMI 12,936, however, Isomer 201 did show significant cross-reaction in the competitive protein-binding assay for progesterone and did not cause significant reduction in peripheral progesterone levels on Day 9 after administration on Day 8. From this and previous evidence it is concluded that RMI 12,936 inhibits progesterone synthesis, possibly by acting as an alternative substrate for ovarian delta(5)3-ketosteroid isomerase, and that the product is probably a competitive antagonist of progesterone at the receptor level.", "PMID": 966221}
{"id": "pubmed23n0032_6638", "title": "Plasma concentrations of testosterone in the developing ram in different social environments.", "content": "Plasma testosterone profiles were determined by taking frequent blood samples at intervals from birth to 21 months of age from rams reared in isolation, in an all-male group and in a mixed-sex group. The testosterone pattern was not modified by these different social environments. The ability of the ram to copulate when first exposed to an oestrous ewe, apparently a maturational process, was also independent of social rearing. At birth plasma testosterone levels were detectable but low, they were higher at 10 and 16 weeks of age and showed a marked rise by 26 weeks, coincident with the time of puberty. Depressive effects of season on testosterone profiles occurred during the 2nd year of life but not the 1st year.", "contents": "Plasma concentrations of testosterone in the developing ram in different social environments. Plasma testosterone profiles were determined by taking frequent blood samples at intervals from birth to 21 months of age from rams reared in isolation, in an all-male group and in a mixed-sex group. The testosterone pattern was not modified by these different social environments. The ability of the ram to copulate when first exposed to an oestrous ewe, apparently a maturational process, was also independent of social rearing. At birth plasma testosterone levels were detectable but low, they were higher at 10 and 16 weeks of age and showed a marked rise by 26 weeks, coincident with the time of puberty. Depressive effects of season on testosterone profiles occurred during the 2nd year of life but not the 1st year.", "PMID": 966222}
{"id": "pubmed23n0032_6639", "title": "Effect of administration of progesterone and oestrogen on litter size in pigs.", "content": "Progesterone and oestrone alone or in combination were administered to sows during early gestation. A dose of 25 mg progesterone and 12-5 mug oestrone injected together for 10,5 or 2 consecutive days during the implantation interval caused increased litter size at term.", "contents": "Effect of administration of progesterone and oestrogen on litter size in pigs. Progesterone and oestrone alone or in combination were administered to sows during early gestation. A dose of 25 mg progesterone and 12-5 mug oestrone injected together for 10,5 or 2 consecutive days during the implantation interval caused increased litter size at term.", "PMID": 966227}
{"id": "pubmed23n0032_6640", "title": "Uterine proteins and development in vitro of rabbit preimplantation embryos.", "content": "The effects of isolated protein fractions from rabbit uteri (prealbumin, albumin, uteroglobin, and beta-glycoprotein), unfractionated uterine proteins, progesterone, oestradiol-17beta, and prostaglandin F-2a on the development of rabbit embryos in vitro were investigated. When exposed to individual protein fractions obtained from Day-6 uteri, 8-cell embryos did not develop into early blastocysts; morulae readily developed into early blastocysts, but further development was retarded. Progesterone (10(-5)-10(-11)M) and prostaglandin F-2a (0-1-10 ng/ml) added to the medium slowed development of blastocysts to advanced stages. Growth of 8- to 16-cell embryos, morulae, and Day-4 blastocysts was stimulated by unfractionated uterine proteins obtained from Day-5 uterine flushings. Although embryos cultured in medium containing BSA had similar rates of blastocyst formation and, ultimately similar blastocyst expansion as did the embryos cultured in medium with unfractionated proteins, the radial and immediate expansion of the early blastocysts cultured in the latter approximated that found in utero.", "contents": "Uterine proteins and development in vitro of rabbit preimplantation embryos. The effects of isolated protein fractions from rabbit uteri (prealbumin, albumin, uteroglobin, and beta-glycoprotein), unfractionated uterine proteins, progesterone, oestradiol-17beta, and prostaglandin F-2a on the development of rabbit embryos in vitro were investigated. When exposed to individual protein fractions obtained from Day-6 uteri, 8-cell embryos did not develop into early blastocysts; morulae readily developed into early blastocysts, but further development was retarded. Progesterone (10(-5)-10(-11)M) and prostaglandin F-2a (0-1-10 ng/ml) added to the medium slowed development of blastocysts to advanced stages. Growth of 8- to 16-cell embryos, morulae, and Day-4 blastocysts was stimulated by unfractionated uterine proteins obtained from Day-5 uterine flushings. Although embryos cultured in medium containing BSA had similar rates of blastocyst formation and, ultimately similar blastocyst expansion as did the embryos cultured in medium with unfractionated proteins, the radial and immediate expansion of the early blastocysts cultured in the latter approximated that found in utero.", "PMID": 966234}
{"id": "pubmed23n0032_6641", "title": "Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or-196 degrees C for various periods.", "content": "Rabbit spermatozoa stored at 37 degrees C for 5 hr, 5 degrees C for 5 hr or 5 days, and-196 degrees C for 5 hr, 5 days, or 1 year were used to artificially inseminate does induced to ovulate normal or excess numbers of eggs. Fertility and subsequent embryonic development were examined. Frozen spermatozoa stored for 5 hr fertilized fewer oocytes than those stored for 5 days or longer. Fewer embryos were recovered on Day 6 than on Day 2. Blastocysts were smaller when spermatozoa were stored for 5 days, were frozen or were placed in superovulating does, but no statistically significant differences in the % of fetal survival were found in the groups of normally ovulating does. Embryonic wastage associated with storage of spermatozoa resulted from a reduced fertilization rate and/or increased embryonic mortality before implantation.", "contents": "Embryonic development in rabbits after insemination with spermatozoa stored at 37, 5 or-196 degrees C for various periods. Rabbit spermatozoa stored at 37 degrees C for 5 hr, 5 degrees C for 5 hr or 5 days, and-196 degrees C for 5 hr, 5 days, or 1 year were used to artificially inseminate does induced to ovulate normal or excess numbers of eggs. Fertility and subsequent embryonic development were examined. Frozen spermatozoa stored for 5 hr fertilized fewer oocytes than those stored for 5 days or longer. Fewer embryos were recovered on Day 6 than on Day 2. Blastocysts were smaller when spermatozoa were stored for 5 days, were frozen or were placed in superovulating does, but no statistically significant differences in the % of fetal survival were found in the groups of normally ovulating does. Embryonic wastage associated with storage of spermatozoa resulted from a reduced fertilization rate and/or increased embryonic mortality before implantation.", "PMID": 966235}
{"id": "pubmed23n0032_6642", "title": "Concentrations of fat and plasma 5alpha-androstenone and plasma testosterone in boars selected for rate of body weight gain and thickness of back fat during growth, sexual maturation and after mating.", "content": "The levels of 5alpha-androstenone and testosterone in peripheral plasma and 5alpha-androstenone in fat collected from 18 boars selected according to thickness of back fat and rate of gain were evaluated. Sampling was started when the animals were about 100 days of age and samples were collected every 14 days until the boars were about 240 days old. The steroid profiles varied considerably between boars, although an increase in steroid levels was found in most animals with the onset of adolescence. The regression line for the level of 5alpha-androstenone in fat and the age of the animals in days was found to be y = 0.02x-1-31. High coefficients of correlation were found between the concentration of 5alpha-androstenone in peripheral plasma and fat (r = 0-78) and between levels of 5alpha-androstenone and testosterone in peripheral plasma (r = 0-64). Levels of 5alpha-androstenone in peripheral plasma above about 15 ng/ml were usually accompanied by a heavy accumulation of 5alpha-androstenone in adipose tissue. The use of four of the boars for mating resulted in increased steroid levels in peripheral plasma and fat. Significantly higher levels of 5alpha-androstenone and testosterone were found in the peripheral plasma of boars selected for fatness and a low rate of gain than in boars selected for low back-fat thickness and a high rate of gain.", "contents": "Concentrations of fat and plasma 5alpha-androstenone and plasma testosterone in boars selected for rate of body weight gain and thickness of back fat during growth, sexual maturation and after mating. The levels of 5alpha-androstenone and testosterone in peripheral plasma and 5alpha-androstenone in fat collected from 18 boars selected according to thickness of back fat and rate of gain were evaluated. Sampling was started when the animals were about 100 days of age and samples were collected every 14 days until the boars were about 240 days old. The steroid profiles varied considerably between boars, although an increase in steroid levels was found in most animals with the onset of adolescence. The regression line for the level of 5alpha-androstenone in fat and the age of the animals in days was found to be y = 0.02x-1-31. High coefficients of correlation were found between the concentration of 5alpha-androstenone in peripheral plasma and fat (r = 0-78) and between levels of 5alpha-androstenone and testosterone in peripheral plasma (r = 0-64). Levels of 5alpha-androstenone in peripheral plasma above about 15 ng/ml were usually accompanied by a heavy accumulation of 5alpha-androstenone in adipose tissue. The use of four of the boars for mating resulted in increased steroid levels in peripheral plasma and fat. Significantly higher levels of 5alpha-androstenone and testosterone were found in the peripheral plasma of boars selected for fatness and a low rate of gain than in boars selected for low back-fat thickness and a high rate of gain.", "PMID": 966236}
{"id": "pubmed23n0032_6643", "title": "Production, chemical composition and prostaglandin F-2alpha content of uterine fluid in pregnant sheep.", "content": "A uterine pouch was prepared surgically in ewes before mating to study the production and chemical composition of uterine secretions during pregnancy. Pregnancy was established in 6 sheep and in the first 55 days uterine fluid was present in small amounts (less than 5 ml), whereas between 109 days post coitum and 3 days post partum the volume ranged from 90 to 775 ml. The chemical composition of uterine fluid in pregnancy differed from that of plasma especially in respect of its high concentration of total calcium (up to 83.5 mM) and prostaglandin (PG) F-2a (up to 1500 ng/ml). Progesterone was implicated as an important endocrine factor since uterine fluid (188 ml) with a high concentration of total calcium (53-5 mM) and PGF-2a(235ng/ml) was recovered from a non-pregnant sheep treated with progesterone for 115 days.", "contents": "Production, chemical composition and prostaglandin F-2alpha content of uterine fluid in pregnant sheep. A uterine pouch was prepared surgically in ewes before mating to study the production and chemical composition of uterine secretions during pregnancy. Pregnancy was established in 6 sheep and in the first 55 days uterine fluid was present in small amounts (less than 5 ml), whereas between 109 days post coitum and 3 days post partum the volume ranged from 90 to 775 ml. The chemical composition of uterine fluid in pregnancy differed from that of plasma especially in respect of its high concentration of total calcium (up to 83.5 mM) and prostaglandin (PG) F-2a (up to 1500 ng/ml). Progesterone was implicated as an important endocrine factor since uterine fluid (188 ml) with a high concentration of total calcium (53-5 mM) and PGF-2a(235ng/ml) was recovered from a non-pregnant sheep treated with progesterone for 115 days.", "PMID": 966237}
{"id": "pubmed23n0032_6644", "title": "Placental fine structure after experimental embryonic death in cows.", "content": "Early embryonic death was experimentally induced by amnion rupture in pregnant heifers. The subsequent morphological alterations in the placenta were studied with light and electron microscopy, histochemical tests and electron microscope microanalysis. The material from the treated heifers differed from that inuntreated cows in the following aspects: the fetal-maternal junction was firm; many endometrial epithelial cells were desquamated and the remaining epithelium showed vacuolization or depletion of organelles; the trophoblast remained at the preattachment stage in its marked phagocytic activity and accumulation of lipid and glycogen, and many of the organelles were degenerated; and the mesenchyme contained extracellular deposits of iron and calcium. It is believed that the changes observed were caused by interruption of the fetal circulation followed by an accumulation of metabolic products.", "contents": "Placental fine structure after experimental embryonic death in cows. Early embryonic death was experimentally induced by amnion rupture in pregnant heifers. The subsequent morphological alterations in the placenta were studied with light and electron microscopy, histochemical tests and electron microscope microanalysis. The material from the treated heifers differed from that inuntreated cows in the following aspects: the fetal-maternal junction was firm; many endometrial epithelial cells were desquamated and the remaining epithelium showed vacuolization or depletion of organelles; the trophoblast remained at the preattachment stage in its marked phagocytic activity and accumulation of lipid and glycogen, and many of the organelles were degenerated; and the mesenchyme contained extracellular deposits of iron and calcium. It is believed that the changes observed were caused by interruption of the fetal circulation followed by an accumulation of metabolic products.", "PMID": 966238}
{"id": "pubmed23n0032_6645", "title": "Macromolecular components of genital tract fluids from the sheep.", "content": "The proteins and glycosidases in the luminal fluid from the genital tract of ewes at different stages of the oestrous cycle and early pregnancy were studied. Most of the proteins in oviducal fluid and uterine fluid were serum proteins, but an increased amount of a glycoprotein excluded from Sephadex G-200 was detected in oviducal fluid at oestrus. Small amounts of uterine-specific proteins were detected in uterine fluid by polyacrylamide gel electrophoresis and  isoelectric focusing. The number of these varied according to the stage of pregnancy. The activities of several glycosidases were elevated in uterine fluid when compared with serum; beta-N-acetylgalactosaminidase and beta-N-acetylglucosamindase were particularly active. Ovine cervical mucus was shown to contain an epithelial glycoprotein which had a composition similar to that of the glycoproteins isolated from human and bovine cervical mucus.", "contents": "Macromolecular components of genital tract fluids from the sheep. The proteins and glycosidases in the luminal fluid from the genital tract of ewes at different stages of the oestrous cycle and early pregnancy were studied. Most of the proteins in oviducal fluid and uterine fluid were serum proteins, but an increased amount of a glycoprotein excluded from Sephadex G-200 was detected in oviducal fluid at oestrus. Small amounts of uterine-specific proteins were detected in uterine fluid by polyacrylamide gel electrophoresis and  isoelectric focusing. The number of these varied according to the stage of pregnancy. The activities of several glycosidases were elevated in uterine fluid when compared with serum; beta-N-acetylgalactosaminidase and beta-N-acetylglucosamindase were particularly active. Ovine cervical mucus was shown to contain an epithelial glycoprotein which had a composition similar to that of the glycoproteins isolated from human and bovine cervical mucus.", "PMID": 966239}
{"id": "pubmed23n0032_6646", "title": "Intrauterine contraception with the copper T-200 and the Dalkon shield in nulligravid women.", "content": "A randomized, prospective study design compares the small Dalkon shield and the Copper T 200 (T Cu 200) intrauterine contraceptive devices in nulligravid women in the same clinic population during a 12-month period. This prospective study demonstrates statistically significant differences in the accidental pregnancy, expulsion and medical removal rates between the two devices. In nulligravid patients the T Cu 200 had lower event rates than the small shield in the categories of accidental pregnancy and medical removals for bleeding and pain.", "contents": "Intrauterine contraception with the copper T-200 and the Dalkon shield in nulligravid women. A randomized, prospective study design compares the small Dalkon shield and the Copper T 200 (T Cu 200) intrauterine contraceptive devices in nulligravid women in the same clinic population during a 12-month period. This prospective study demonstrates statistically significant differences in the accidental pregnancy, expulsion and medical removal rates between the two devices. In nulligravid patients the T Cu 200 had lower event rates than the small shield in the categories of accidental pregnancy and medical removals for bleeding and pain.", "PMID": 966243}
{"id": "pubmed23n0032_6647", "title": "Cysteine derivatives with reactive groups as potential antitumor agents.", "content": "Cysteine derivatives having diazo ketone and chloro ketone functions have been prepared. In order to effect adequate protection for modifying the carboxyl group, cysteine was converted to a thiazolidine derivative I, which was then converted to the N-acetyl derivative VII. The active ester method or activation with DCC yielded the diazo ketone derivative VIII. Similar treatment of the parent compound I with DCC led to a self-condensation reaction giving a diketopiperazine VI. The diazo ketone derivative VIII has been used in preparing alpha-chloro ketone derivative X and a homologue of cysteine. Deblocking N-acetylated thiazolidine derivatives with various reagents did not proceed satisfactorily. Interaction of the N-acetylated blocked ester XII with trifluoroacetic acid opened the thiazolidine ring to give the N-acetylated blocked diester XIII and other products. The chloro ketone derivative X was found to have a moderate inhibitory activity against mouse mammary adenocarcinoma in cell culture. S-(1-Adamantyl)-L-cysteine was prepared and was found to be inactive.", "contents": "Cysteine derivatives with reactive groups as potential antitumor agents. Cysteine derivatives having diazo ketone and chloro ketone functions have been prepared. In order to effect adequate protection for modifying the carboxyl group, cysteine was converted to a thiazolidine derivative I, which was then converted to the N-acetyl derivative VII. The active ester method or activation with DCC yielded the diazo ketone derivative VIII. Similar treatment of the parent compound I with DCC led to a self-condensation reaction giving a diketopiperazine VI. The diazo ketone derivative VIII has been used in preparing alpha-chloro ketone derivative X and a homologue of cysteine. Deblocking N-acetylated thiazolidine derivatives with various reagents did not proceed satisfactorily. Interaction of the N-acetylated blocked ester XII with trifluoroacetic acid opened the thiazolidine ring to give the N-acetylated blocked diester XIII and other products. The chloro ketone derivative X was found to have a moderate inhibitory activity against mouse mammary adenocarcinoma in cell culture. S-(1-Adamantyl)-L-cysteine was prepared and was found to be inactive.", "PMID": 966244}
{"id": "pubmed23n0032_6648", "title": "Synthesis and biological evaluation of omega-homologues of prostaglandin E1.", "content": "The synthesis and biological activities of some compounds with novel modifications of the omega side chain of prostaglandin E1 (PGE1) are described. The preparation of (+/-)-omega-Me-PGE1 (3) (+/-)-omega-Pr-PGE1 (5), and (+/-)-omega-Bu-PGE1 (6) is outlined. The compounds were evaluated for in vitro smooth muscle stimulating activity on isolated gerbil colon preparations, for hypotensive action in anesthetized rats, and for gastric antisecretory effects in histamine-stimulated Heidenhain pouch dogs. Structural changes in the omega position of the noncarboxyl side chain of PGE1 influenced the biological potency of the resulting compound when compared to the reference standard PGE1 (2). The homologues demonstrated interesting separation of biological activities; for example, 4 showed potent hypotensive activity (84% of PGE1, it showed very low smooth muscle stimulating activity. Compound 3 possessed the same order of potency as 2 in the gastric antisecretory assay.", "contents": "Synthesis and biological evaluation of omega-homologues of prostaglandin E1. The synthesis and biological activities of some compounds with novel modifications of the omega side chain of prostaglandin E1 (PGE1) are described. The preparation of (+/-)-omega-Me-PGE1 (3) (+/-)-omega-Pr-PGE1 (5), and (+/-)-omega-Bu-PGE1 (6) is outlined. The compounds were evaluated for in vitro smooth muscle stimulating activity on isolated gerbil colon preparations, for hypotensive action in anesthetized rats, and for gastric antisecretory effects in histamine-stimulated Heidenhain pouch dogs. Structural changes in the omega position of the noncarboxyl side chain of PGE1 influenced the biological potency of the resulting compound when compared to the reference standard PGE1 (2). The homologues demonstrated interesting separation of biological activities; for example, 4 showed potent hypotensive activity (84% of PGE1, it showed very low smooth muscle stimulating activity. Compound 3 possessed the same order of potency as 2 in the gastric antisecretory assay.", "PMID": 966245}
{"id": "pubmed23n0032_6649", "title": "Tyrosylated analogues of somatostatin.", "content": "The total synthesis of [tyr1]-, [tyr6]-, E1Tyr7], [d-tyr8]-, [tyr11]-,and [tyr1,D-Trp8]somatostatin is described. The biological potencies of these analogues in vitro (inhibition of spontaneous secretion of radioimmunoassayable growth hormone by cultured rat anterior pituitary cells) as compared with that of somatostatin (100) were found to be 116, 29, 108, 10, 65, and 400, respectively; in vivo, in the rat, these peptides were assayed for their ability to inhibit the spontaneous release of insulin and glucagon. No statistically significant discrepancies were observed in the potency values obtained in vitro and in vivo further indicating similarities of specificities and sensitivities of the pituitary and pancreatotropic receptors.", "contents": "Tyrosylated analogues of somatostatin. The total synthesis of [tyr1]-, [tyr6]-, E1Tyr7], [d-tyr8]-, [tyr11]-,and [tyr1,D-Trp8]somatostatin is described. The biological potencies of these analogues in vitro (inhibition of spontaneous secretion of radioimmunoassayable growth hormone by cultured rat anterior pituitary cells) as compared with that of somatostatin (100) were found to be 116, 29, 108, 10, 65, and 400, respectively; in vivo, in the rat, these peptides were assayed for their ability to inhibit the spontaneous release of insulin and glucagon. No statistically significant discrepancies were observed in the potency values obtained in vitro and in vivo further indicating similarities of specificities and sensitivities of the pituitary and pancreatotropic receptors.", "PMID": 966246}
{"id": "pubmed23n0032_6650", "title": "Nucleic acids. 16. Orally active derivatives of ara-cytidine.", "content": "Water-soluble derivatives of ara-cytidine (cytarabine, Cytosar) were prepared and tested for antitumor, immunosuppressive, and antiarthritic activities in animals after oral administration. The compounds tested included the 5'-palmitate, 5'-benzoate, and 5'-adamantoate esters of ara-cytidine, made water soluble by use of their hydrochloride salts of peptidyl derivatives, and two basic 5' esters (5'-nicotinoate and 5'-quinuclidinate) as their hydrochloride salts. Five of the compounds had antitumor activity superior to that found with ara-cytidine itself after oral administration in the L1210 leukemic mouse assay. One of these, 5'-adamantoyl-ara-cytidine hydrochloride, had antitumor activity after oral administration approaching that achieved with parenterally administered ara-cytidine.", "contents": "Nucleic acids. 16. Orally active derivatives of ara-cytidine. Water-soluble derivatives of ara-cytidine (cytarabine, Cytosar) were prepared and tested for antitumor, immunosuppressive, and antiarthritic activities in animals after oral administration. The compounds tested included the 5'-palmitate, 5'-benzoate, and 5'-adamantoate esters of ara-cytidine, made water soluble by use of their hydrochloride salts of peptidyl derivatives, and two basic 5' esters (5'-nicotinoate and 5'-quinuclidinate) as their hydrochloride salts. Five of the compounds had antitumor activity superior to that found with ara-cytidine itself after oral administration in the L1210 leukemic mouse assay. One of these, 5'-adamantoyl-ara-cytidine hydrochloride, had antitumor activity after oral administration approaching that achieved with parenterally administered ara-cytidine.", "PMID": 966247}
{"id": "pubmed23n0032_6651", "title": "Synthesis of tetrazole ribonucleosides and their evaluation as antiviral agents. 2. 5-Amino-1-(beta-D-ribofuranosyl)-1H-tetrazole and 5-amino-2-(beta-D-ribofuranosyl)-2H-tetrazole.", "content": "Synthesis of 5-amino-1-(beta-D-ribofuranosyl)-1H-tetrazole and 5-amino-2-(beta-D-ribofuranosyl)-2H-tetrazole is described. X-Ray crystallography was first used to establish the stereochemical configuration of the two isomers. By conducting 13C NMR analysis on these isomers with known structures, i.e., N1beta, a correlation is developed for determining the N-ribosyl attachment site of tetrazole ribonucleosides. Results are also presented on antiviral testing of these synthetic 5-aminotetrazole ribonucleosides against influenza A2/Asian/J305 virus infection in mice.", "contents": "Synthesis of tetrazole ribonucleosides and their evaluation as antiviral agents. 2. 5-Amino-1-(beta-D-ribofuranosyl)-1H-tetrazole and 5-amino-2-(beta-D-ribofuranosyl)-2H-tetrazole. Synthesis of 5-amino-1-(beta-D-ribofuranosyl)-1H-tetrazole and 5-amino-2-(beta-D-ribofuranosyl)-2H-tetrazole is described. X-Ray crystallography was first used to establish the stereochemical configuration of the two isomers. By conducting 13C NMR analysis on these isomers with known structures, i.e., N1beta, a correlation is developed for determining the N-ribosyl attachment site of tetrazole ribonucleosides. Results are also presented on antiviral testing of these synthetic 5-aminotetrazole ribonucleosides against influenza A2/Asian/J305 virus infection in mice.", "PMID": 966248}
{"id": "pubmed23n0032_6652", "title": "Design of substrate-site-directed irreversible inhibitors of adenosine 5'-phosphate aminohydrolase. Effect of substrate substituents on affinity for the substrate site.", "content": "Derivatives of adenosine 5'-phosphate (AMP) have been synthesized in which the phosphoester (POCH2) grouping of AMP is replaced by PCH(R)CH2 where R is OC(O)Me, CH2NHCOMe, CH2NHCOEt, and CH2NHCOOR' (R' = Me, Et, and Pr). The 2',3'-O-isopropylidene and 2', 3'-di-O-acetyl derivatives of AMP were also prepared. All compounds were competitive inhibitors of rabbit muscle AMP aminohydrolase with enzyme-inhibitor dissociation constants (Ki values) of 330, 20, 17, 19, 16, 14, 260, and 105 muM, respectively. All compounds were substrates except those in which R was CH2NHCOEt and CH2NHCOOR' (R'=Me, Et, and Pr). The previously described allo and talo epimers of 5'-C-acetylaminomethyl-AMP and the allo epimer of 5'-C-propionylaminomethyl-AMP were substrates and competitive inhibitors with Ki values of 18, 47, and 42 muM, respectively. The talo epimer of 5'-C-propionylaminomethyl-AMP was not a substrate and was a noncompetitive inhibitor, Ki", "contents": "Design of substrate-site-directed irreversible inhibitors of adenosine 5'-phosphate aminohydrolase. Effect of substrate substituents on affinity for the substrate site. Derivatives of adenosine 5'-phosphate (AMP) have been synthesized in which the phosphoester (POCH2) grouping of AMP is replaced by PCH(R)CH2 where R is OC(O)Me, CH2NHCOMe, CH2NHCOEt, and CH2NHCOOR' (R' = Me, Et, and Pr). The 2',3'-O-isopropylidene and 2', 3'-di-O-acetyl derivatives of AMP were also prepared. All compounds were competitive inhibitors of rabbit muscle AMP aminohydrolase with enzyme-inhibitor dissociation constants (Ki values) of 330, 20, 17, 19, 16, 14, 260, and 105 muM, respectively. All compounds were substrates except those in which R was CH2NHCOEt and CH2NHCOOR' (R'=Me, Et, and Pr). The previously described allo and talo epimers of 5'-C-acetylaminomethyl-AMP and the allo epimer of 5'-C-propionylaminomethyl-AMP were substrates and competitive inhibitors with Ki values of 18, 47, and 42 muM, respectively. The talo epimer of 5'-C-propionylaminomethyl-AMP was not a substrate and was a noncompetitive inhibitor, Ki", "PMID": 966249}
{"id": "pubmed23n0032_6653", "title": "Theoretical model-based equations for the linear free energy relationships of the biological activity of ionizable substances. 1. Equilibrium-controlled potency.", "content": "Because of the ambiguities of how to treat ionization in empirical equations which relate biological activity to partition coefficient by use of a (log P)2 term, a theoretical approach to the problem is proposed. Based on a simplified view of assays of potency following in vitro or continuous infusion administration of drugs, equations have been derived from a combination of mass law, equilibrium, and extrathermodynamic assumptions. In general form the equations which relate potency to partition coefficient (P) and degree of ionization (alpha) are the following. If the neutral form reacts with the receptor, log (1/C) =-log [1 + SIGMAM(DIPci) + sigman[aj/Pb(1-alpha4y]] + X. If the ionic form reacts with the receptor, log (1/C) =-log [1 + (1 - Alphan)/(alphan)[sigmam(diPci) + sigman[aj/Pb(1-alphaj)]]] + X. In this generalized model there are m nonaqueous compartments and n aqueous compartments of different pH. The parameters a, b, c, and d can be interpreted in terms of the model. The shape of the log (1/C) vs. log P curve may be asymptotic, linear, or composed of two portions of unequal slope which meet at an optimum or a bend. With the use of these equations it is possible to examine whether the ion or the neutral form is the active species and whether there is hydrophobic bonding to the receptor and/or an inert compartment. The models may be further extended to include terms other than log P and alpha.", "contents": "Theoretical model-based equations for the linear free energy relationships of the biological activity of ionizable substances. 1. Equilibrium-controlled potency. Because of the ambiguities of how to treat ionization in empirical equations which relate biological activity to partition coefficient by use of a (log P)2 term, a theoretical approach to the problem is proposed. Based on a simplified view of assays of potency following in vitro or continuous infusion administration of drugs, equations have been derived from a combination of mass law, equilibrium, and extrathermodynamic assumptions. In general form the equations which relate potency to partition coefficient (P) and degree of ionization (alpha) are the following. If the neutral form reacts with the receptor, log (1/C) =-log [1 + SIGMAM(DIPci) + sigman[aj/Pb(1-alpha4y]] + X. If the ionic form reacts with the receptor, log (1/C) =-log [1 + (1 - Alphan)/(alphan)[sigmam(diPci) + sigman[aj/Pb(1-alphaj)]]] + X. In this generalized model there are m nonaqueous compartments and n aqueous compartments of different pH. The parameters a, b, c, and d can be interpreted in terms of the model. The shape of the log (1/C) vs. log P curve may be asymptotic, linear, or composed of two portions of unequal slope which meet at an optimum or a bend. With the use of these equations it is possible to examine whether the ion or the neutral form is the active species and whether there is hydrophobic bonding to the receptor and/or an inert compartment. The models may be further extended to include terms other than log P and alpha.", "PMID": 966250}
{"id": "pubmed23n0032_6654", "title": "Quantitative structure-activity relationships. 3.1 A comparison of different Free-Wilson models.", "content": "The Fujita-Ban model and the classical Free-Wilson model are shown to be linearly related: the de novo group contributions obtained by one model are linear transformations of those obtained by the other model. An example is given to illustrate this linear dependence. The Fujita-Ban model is characterized by a number of advantages as compared with the classical Free-Wilson model: no transformation of the structural matrix and no symmetry equations are necessary; all group contributions are based on an arbitrarily chosen reference compound, preferably the unsubstituted compound; the constant term, which is the theoretically predicted activity value of the reference compound, and the values of the group contributions are not markedly influenced by addition or elimination of a compound; the problem of linear dependence (the singularity problem) sometimes can be circumvented by preparation of a contracted matrix; if the unsubstituted compound is chosen as reference compound, the group contributions are numerically equivalent to Hansch-derived group contributions; therefore, the Hansch approach and the Fujita-Ban model can be combined to a mixed approach. Taking all these facts into consideration, the Frujita-Ban model is recommended as the most suitable approach for the calculation of de novo group contributions.", "contents": "Quantitative structure-activity relationships. 3.1 A comparison of different Free-Wilson models. The Fujita-Ban model and the classical Free-Wilson model are shown to be linearly related: the de novo group contributions obtained by one model are linear transformations of those obtained by the other model. An example is given to illustrate this linear dependence. The Fujita-Ban model is characterized by a number of advantages as compared with the classical Free-Wilson model: no transformation of the structural matrix and no symmetry equations are necessary; all group contributions are based on an arbitrarily chosen reference compound, preferably the unsubstituted compound; the constant term, which is the theoretically predicted activity value of the reference compound, and the values of the group contributions are not markedly influenced by addition or elimination of a compound; the problem of linear dependence (the singularity problem) sometimes can be circumvented by preparation of a contracted matrix; if the unsubstituted compound is chosen as reference compound, the group contributions are numerically equivalent to Hansch-derived group contributions; therefore, the Hansch approach and the Fujita-Ban model can be combined to a mixed approach. Taking all these facts into consideration, the Frujita-Ban model is recommended as the most suitable approach for the calculation of de novo group contributions.", "PMID": 966251}
{"id": "pubmed23n0032_6655", "title": "(-)-3-Isothujone, a small nonnitrogenous molecule with antinociceptive activity in mice.", "content": "(-)-3-Isothujone and (+)-3-thujone were examined for antinociceptive activity using the hot-plate and Nilsen tests. In the hot plate test (-)-3-isothujone (ED50 = 6.5 mg/kg) was found to be codeine-like and equipotent with (-)-delta9-tetrahydrocannabinol while the racemic material was essentially half as potent as the levoratatory isomer. (+)-3-Thujone was inactive in both antinociceptive tests as were several structural analogues of the 3-thujones. As with the THC's less antinociceptive activity was observed in the Nilsen test than in the hot-plate assay. Acute toxicities for the 3-thujones were determined and vastly improved synthetic procedures have been developed for two long-known but difficulty accessible 3-thujanols.", "contents": "(-)-3-Isothujone, a small nonnitrogenous molecule with antinociceptive activity in mice. (-)-3-Isothujone and (+)-3-thujone were examined for antinociceptive activity using the hot-plate and Nilsen tests. In the hot plate test (-)-3-isothujone (ED50 = 6.5 mg/kg) was found to be codeine-like and equipotent with (-)-delta9-tetrahydrocannabinol while the racemic material was essentially half as potent as the levoratatory isomer. (+)-3-Thujone was inactive in both antinociceptive tests as were several structural analogues of the 3-thujones. As with the THC's less antinociceptive activity was observed in the Nilsen test than in the hot-plate assay. Acute toxicities for the 3-thujones were determined and vastly improved synthetic procedures have been developed for two long-known but difficulty accessible 3-thujanols.", "PMID": 966252}
{"id": "pubmed23n0032_6656", "title": "Optical isomers of 2-(2-ethoxyphenoxymethyl)tetrahydro-1,4-oxazine (viloxazine) and related compounds.", "content": "The optical isomers of 2-(2-ethoxyphenoxymethyl)tetrahydro-1,4-oxazine (viloxazine) and 2-(3-methoxyphenoxymethyl)tetrahydro-1,4-oxazine have been prepared and absolute configurations have been assigned. In their action on the central nervous system the S isomers are at least ten times more potent than the R isomers. The intermediate 4-benzyl-2-(p-toluensulfonyloxymethyl)tetrahydro-1,4-oxazine has been resolved. Its isomers provide a convenient starting point for the preparation of optical isomers of viloxazine analogues of known configuration.", "contents": "Optical isomers of 2-(2-ethoxyphenoxymethyl)tetrahydro-1,4-oxazine (viloxazine) and related compounds. The optical isomers of 2-(2-ethoxyphenoxymethyl)tetrahydro-1,4-oxazine (viloxazine) and 2-(3-methoxyphenoxymethyl)tetrahydro-1,4-oxazine have been prepared and absolute configurations have been assigned. In their action on the central nervous system the S isomers are at least ten times more potent than the R isomers. The intermediate 4-benzyl-2-(p-toluensulfonyloxymethyl)tetrahydro-1,4-oxazine has been resolved. Its isomers provide a convenient starting point for the preparation of optical isomers of viloxazine analogues of known configuration.", "PMID": 966254}
{"id": "pubmed23n0032_6657", "title": "Synthesis and biological properties of 4-amino- and 4-bromo-4-norpyridoxol. New approaches for the modification of the 4 position of vitamin B6.", "content": "4-Amino-4-norpyridoxol, a new key intermediate for the modification of the 4 position of vitamin B6, has been obtained by an unusual photochemical rearrangement of pyridoxal oxime. It has also been synthesized starting from 3,-alpha5-O-dibenzylpyridoxal, which was converted to 3,alpha5-O-dibenzylpyridoxamide. The latter, on Hoffman reaction, gave the desired 3,5-blocked 4-amino derivative. Several derivatives of this analogue have been prepared, and its existence in the amino tautomeric form has been established by NMR spectroscopy. A modified Sandmeyer reaction on 4-amino-4-norpyridoxol gave the 4-bromo analogue, which was found to be moderately active as an inhibitor of mouse mammary adenocarcinoma cells grown in cell culture, whereas the 4-amino analogue was not active at 10(-4)M. Other analogues containing electron-withdrawing and electron-donating substituents in the 4 position of pyridoxine were also tested.", "contents": "Synthesis and biological properties of 4-amino- and 4-bromo-4-norpyridoxol. New approaches for the modification of the 4 position of vitamin B6. 4-Amino-4-norpyridoxol, a new key intermediate for the modification of the 4 position of vitamin B6, has been obtained by an unusual photochemical rearrangement of pyridoxal oxime. It has also been synthesized starting from 3,-alpha5-O-dibenzylpyridoxal, which was converted to 3,alpha5-O-dibenzylpyridoxamide. The latter, on Hoffman reaction, gave the desired 3,5-blocked 4-amino derivative. Several derivatives of this analogue have been prepared, and its existence in the amino tautomeric form has been established by NMR spectroscopy. A modified Sandmeyer reaction on 4-amino-4-norpyridoxol gave the 4-bromo analogue, which was found to be moderately active as an inhibitor of mouse mammary adenocarcinoma cells grown in cell culture, whereas the 4-amino analogue was not active at 10(-4)M. Other analogues containing electron-withdrawing and electron-donating substituents in the 4 position of pyridoxine were also tested.", "PMID": 966255}
{"id": "pubmed23n0032_6658", "title": "Justice, welfare and health care.", "content": "Miss Telfer offers a new analysis, classifying health care into four systems, only one of which, the \"laissez-faire\" type, is unlikely to be acceptable today. The other three systems are defined here as \"liberal humanitarian\", \"liberal socialist\" and \"pure socialist.\" Each is analysed for its content and for the views of its protagonists and antagonists. On these issues no dogma is proclaimed as the author says she has sought to \"bring out some of the principles at issue in any discussion of the rights and wrongs of socialized medicine\". This journal is surely the proper place for such a discussion as the worlds of the politician, of the economist, of the doctor and of the patient come to a point in the philosophies behind the aspect of medical ethics exemplified in the provision of medical services by the state. Miss Telfer also glances down the byways of the medicine of the market place.", "contents": "Justice, welfare and health care. Miss Telfer offers a new analysis, classifying health care into four systems, only one of which, the \"laissez-faire\" type, is unlikely to be acceptable today. The other three systems are defined here as \"liberal humanitarian\", \"liberal socialist\" and \"pure socialist.\" Each is analysed for its content and for the views of its protagonists and antagonists. On these issues no dogma is proclaimed as the author says she has sought to \"bring out some of the principles at issue in any discussion of the rights and wrongs of socialized medicine\". This journal is surely the proper place for such a discussion as the worlds of the politician, of the economist, of the doctor and of the patient come to a point in the philosophies behind the aspect of medical ethics exemplified in the provision of medical services by the state. Miss Telfer also glances down the byways of the medicine of the market place.", "PMID": 966258}
{"id": "pubmed23n0032_6659", "title": "The ethics of animal experimentation.", "content": "Animal experimentation arouses great emotion in many people, perhaps more especially in Britain, and this has increased as more sophisticated medical and non-medical animal experiments are demanded by modern research. The Cruelty to Animals Act of 1876 is the only legal regulation of experiments in animals, and many of its clauses are ambiguous. So in 1963 a committee of enquiry - the Littlewood Committee - was set up. Dr Lane-Petter examines the emotional and factual background to the enquiry, and discusses in an ethical context the usefulness and positive advantages of animal experiments compared with those of possible substitutes and in some detail three of the questions left unanswered by the Littlewood Committee.", "contents": "The ethics of animal experimentation. Animal experimentation arouses great emotion in many people, perhaps more especially in Britain, and this has increased as more sophisticated medical and non-medical animal experiments are demanded by modern research. The Cruelty to Animals Act of 1876 is the only legal regulation of experiments in animals, and many of its clauses are ambiguous. So in 1963 a committee of enquiry - the Littlewood Committee - was set up. Dr Lane-Petter examines the emotional and factual background to the enquiry, and discusses in an ethical context the usefulness and positive advantages of animal experiments compared with those of possible substitutes and in some detail three of the questions left unanswered by the Littlewood Committee.", "PMID": 966259}
{"id": "pubmed23n0032_6660", "title": "Exorcism: a psychiatric viewpoint.", "content": "Doctors, for several reasons, should be concerned with exorcism is the view of Professor Trethowan, who in this paper, looks at the main features of exorcism as practised in the middle ages and now appearing in the modern world, as was seen in the recent Ossett case in Britain. He examines in some detail the nature of supposed demoniacal possession and describes its symptoms and signs. He also touches on the social, as opposed to the religious, background in which demoniacal possession flourished (not lacking in the world today), so leading to an examination of the psychodynamic aspects of demoniacal possession and the question of absolute evil. Finally he compares the techniques of exorcism and of modern psychiatric practice.", "contents": "Exorcism: a psychiatric viewpoint. Doctors, for several reasons, should be concerned with exorcism is the view of Professor Trethowan, who in this paper, looks at the main features of exorcism as practised in the middle ages and now appearing in the modern world, as was seen in the recent Ossett case in Britain. He examines in some detail the nature of supposed demoniacal possession and describes its symptoms and signs. He also touches on the social, as opposed to the religious, background in which demoniacal possession flourished (not lacking in the world today), so leading to an examination of the psychodynamic aspects of demoniacal possession and the question of absolute evil. Finally he compares the techniques of exorcism and of modern psychiatric practice.", "PMID": 966260}
{"id": "pubmed23n0032_6661", "title": "\"The soft embalmer of the still midnight\": a problem of prescribing hypnotics in general practice.", "content": "Was it euthanasia or was it even murder? That is the rather dramatic question with which the case history ends. However, none of those taking part in this conference had any doubts that it was neither murder nor euthanasia. But all had doubts as to whether the patient received the best care which the medical and allied professions can command. The barrister declared that in law there would have been no case to answer but the consensus of opinion of the other participants was that as no real diagnosis had been made the treatment was essentially superficial - ensuring sleep for the patient by prescribing barbiturates. The most fundamental criticism of the management of this patient was that individuals looked after her rather than an interdisciplinary team, some member of which might properly have sought to overrule Dorothy (the patient's daughter) when she took her mother away from a nursing home. However, what makes this case conference fascinating is not the facts of the case or the treatment so much as the avenues for moral and social discussion opened for the reader.", "contents": "\"The soft embalmer of the still midnight\": a problem of prescribing hypnotics in general practice. Was it euthanasia or was it even murder? That is the rather dramatic question with which the case history ends. However, none of those taking part in this conference had any doubts that it was neither murder nor euthanasia. But all had doubts as to whether the patient received the best care which the medical and allied professions can command. The barrister declared that in law there would have been no case to answer but the consensus of opinion of the other participants was that as no real diagnosis had been made the treatment was essentially superficial - ensuring sleep for the patient by prescribing barbiturates. The most fundamental criticism of the management of this patient was that individuals looked after her rather than an interdisciplinary team, some member of which might properly have sought to overrule Dorothy (the patient's daughter) when she took her mother away from a nursing home. However, what makes this case conference fascinating is not the facts of the case or the treatment so much as the avenues for moral and social discussion opened for the reader.", "PMID": 966261}
{"id": "pubmed23n0032_6662", "title": "Fluctuation and relaxation analysis of monazomycin-induced conductance in black lipid membranes.", "content": "Fluctuation and relaxation analyses were performed on monazomycin-induced conductance of lipid bilayer membranes. With both methods a slow (sec) and a fast (msec) current component are apparent; however, the amplitude of the slow, voltage-dependent process is greater than that of the fast component in the step relaxation experiment and less in the fluctuation experiment. The fluctuation analysis showed principally a rapid voltage-dependent process which appears to be related to the multistate character of the conducting channel. The experimental results are interpreted in terms of a simplified kinetic model which is used to calculate relaxation and noise amplitudes.", "contents": "Fluctuation and relaxation analysis of monazomycin-induced conductance in black lipid membranes. Fluctuation and relaxation analyses were performed on monazomycin-induced conductance of lipid bilayer membranes. With both methods a slow (sec) and a fast (msec) current component are apparent; however, the amplitude of the slow, voltage-dependent process is greater than that of the fast component in the step relaxation experiment and less in the fluctuation experiment. The fluctuation analysis showed principally a rapid voltage-dependent process which appears to be related to the multistate character of the conducting channel. The experimental results are interpreted in terms of a simplified kinetic model which is used to calculate relaxation and noise amplitudes.", "PMID": 966263}
{"id": "pubmed23n0032_6663", "title": "2-Deoxyglucose transport by intestinal epithelial cells isolated from the chick.", "content": "Characteristics of 2-deoxyglucose uptake (2DG) by intestinal epithelial cells isolated from chickens were evaluated as a means of discriminating between the concentrative transport system for monosaccharides, associated with the mucosal brush border, and other possible routes of monosaccharide entry. 2DG was chosen as it is not a substrate for the mucosal transport system. The deoxysugar enters via a saturable pathway which is not Na+-dependent, is not inhibited by K+, does not accumulate solute against a concentration gradient; exhibits a high sensitivity to inhibition by phloretin; is relatively insensitive to phlorizin inhibition; and has low affinity [but high capacity relative to Na+-dependent mucosal transport of 3-O-methylglucose (3-OMG) and other monosaccharides]. These characteristics confirm those established in an earlier report for Na+-independent uptake of 3-OMG. Complications encountered in the use of 2DG as a test sugar include significant rates of metabolic conversion to an anionic form which presumably is a phosphorylated species. Methods for distinguishing between transport and subsequent metabolism are described. Inhibition of 2DG entry by several other sugars is described and inhibitory constants (K's) given for each.", "contents": "2-Deoxyglucose transport by intestinal epithelial cells isolated from the chick. Characteristics of 2-deoxyglucose uptake (2DG) by intestinal epithelial cells isolated from chickens were evaluated as a means of discriminating between the concentrative transport system for monosaccharides, associated with the mucosal brush border, and other possible routes of monosaccharide entry. 2DG was chosen as it is not a substrate for the mucosal transport system. The deoxysugar enters via a saturable pathway which is not Na+-dependent, is not inhibited by K+, does not accumulate solute against a concentration gradient; exhibits a high sensitivity to inhibition by phloretin; is relatively insensitive to phlorizin inhibition; and has low affinity [but high capacity relative to Na+-dependent mucosal transport of 3-O-methylglucose (3-OMG) and other monosaccharides]. These characteristics confirm those established in an earlier report for Na+-independent uptake of 3-OMG. Complications encountered in the use of 2DG as a test sugar include significant rates of metabolic conversion to an anionic form which presumably is a phosphorylated species. Methods for distinguishing between transport and subsequent metabolism are described. Inhibition of 2DG entry by several other sugars is described and inhibitory constants (K's) given for each.", "PMID": 966264}
{"id": "pubmed23n0032_6664", "title": "Effect of transepithelial concentration gradients on the passive fluxes of sodium across toad bladder.", "content": "Bidirectional sodium fluxes across toad bladder were measured after eliminating active transport with ouabain. Mucosal sodium concentration, Cm, was progressively reduced (from 114 to 3 mM) while serosal sodium remained constant. Potential difference was maintained at zero by current passage. The ratio, Q, of the bulk permeability coefficient for sodium, P, to the tracer sodium permeability coefficient, P, was found to remain constant as Cm decreased. Equations were derived on this basis for bidirectional fluxes and for P and P as functions of Cm, which corresponded closely to the observed data. The explanation for the observed value of Q and its constancy under these conditions is uncertain.", "contents": "Effect of transepithelial concentration gradients on the passive fluxes of sodium across toad bladder. Bidirectional sodium fluxes across toad bladder were measured after eliminating active transport with ouabain. Mucosal sodium concentration, Cm, was progressively reduced (from 114 to 3 mM) while serosal sodium remained constant. Potential difference was maintained at zero by current passage. The ratio, Q, of the bulk permeability coefficient for sodium, P, to the tracer sodium permeability coefficient, P, was found to remain constant as Cm decreased. Equations were derived on this basis for bidirectional fluxes and for P and P as functions of Cm, which corresponded closely to the observed data. The explanation for the observed value of Q and its constancy under these conditions is uncertain.", "PMID": 966265}
{"id": "pubmed23n0032_6665", "title": "On the mechanism of electrical coupling between cells of early Xenopus embryos.", "content": "The mechanism of electrical coupling between cells of early Xenopus embryos has been studied by examination of the nonjunctional membrane resistances and capacitances as a function of cleavage stage, the junctional and nonjunctional membrane resistances as functions of time during the first cleavage, and the electrical properties of the primitive blastocoel. The changes in membrane resistances and capacitances during the first two cleavages may be completely explained by the addition of new membrane, identical in specific resistance and capacitance to the original membrane, at a constant rate to furrows which are electrically connected to the perivitelline space. Microelectrode recording from the primitive blastocoel indicates that there is no electrical difference detectable between it and the perivitelline space. These results are discussed in the context of current theories of the mechanism of intercellular electrotonic coupling.", "contents": "On the mechanism of electrical coupling between cells of early Xenopus embryos. The mechanism of electrical coupling between cells of early Xenopus embryos has been studied by examination of the nonjunctional membrane resistances and capacitances as a function of cleavage stage, the junctional and nonjunctional membrane resistances as functions of time during the first cleavage, and the electrical properties of the primitive blastocoel. The changes in membrane resistances and capacitances during the first two cleavages may be completely explained by the addition of new membrane, identical in specific resistance and capacitance to the original membrane, at a constant rate to furrows which are electrically connected to the perivitelline space. Microelectrode recording from the primitive blastocoel indicates that there is no electrical difference detectable between it and the perivitelline space. These results are discussed in the context of current theories of the mechanism of intercellular electrotonic coupling.", "PMID": 966266}
{"id": "pubmed23n0032_6666", "title": "The mechanism of Na+ transport by rabbit urinary bladder.", "content": "The mechanism of Na+ transport in rabbit urinary bladder has been studied by microelectrode techniques. Of the three layers of epithelium, the apical layer contains virtually all the transepithelial resistance. There is radial cell-to-cell coupling within this layer, but there is no detectable transverse coupling between layers. Cell coupling is apparently interrupted by intracellular injection of depolarizing current. The cell interiors are electrically negative to the bathing solutions, but the apical membrane of the apical layer depolarizes with increasing Isc. Voltage scanning detects no current sinks at the cell junctions or elsewhere. The voltage-divider ratio, alpha, (ratio of resistance of apical cell membrane, Ralpha, to basolateral cell membrane, Rb) decreases from 30 to 0.5 with increasing Isc, because of the transport-related conductance pathway in the apical membrane. Changes in effective transepithelial capacitance with Isc are predicted and possibly observed. The transepithelial resistance, Rt, has been resolved into Ra, Rb, and the junctional resistance, Rj, by four different methods: cable analysis, resistance of uncoupled cells, measurements of pairs of (Rt, alpha) values in the same bladder at different transport rates, and the relation between Rt and Isc and between alpha and Isc. Rj proves to be effectively infinite (nominally 300 k omega muF) and independent of Isc, and Ra decreases from 154 to 4 omega muF with increasing Isc. In the resulting model of Na+ transport in \"tight\" epithelia, the apical membrane contains an amiloride-inhibited and Ca++-inhibited conductance pathway for Na+ entry; the basolateral membrane contains a Na+--K+-activated ATPase that extrudes Na+; intracellular (Na+) may exert negative feedback on apical membrane conductance; and aldosterone acts to stimulate Na+ entry at the apical membrane via the amiloride-sensitive pathway.", "contents": "The mechanism of Na+ transport by rabbit urinary bladder. The mechanism of Na+ transport in rabbit urinary bladder has been studied by microelectrode techniques. Of the three layers of epithelium, the apical layer contains virtually all the transepithelial resistance. There is radial cell-to-cell coupling within this layer, but there is no detectable transverse coupling between layers. Cell coupling is apparently interrupted by intracellular injection of depolarizing current. The cell interiors are electrically negative to the bathing solutions, but the apical membrane of the apical layer depolarizes with increasing Isc. Voltage scanning detects no current sinks at the cell junctions or elsewhere. The voltage-divider ratio, alpha, (ratio of resistance of apical cell membrane, Ralpha, to basolateral cell membrane, Rb) decreases from 30 to 0.5 with increasing Isc, because of the transport-related conductance pathway in the apical membrane. Changes in effective transepithelial capacitance with Isc are predicted and possibly observed. The transepithelial resistance, Rt, has been resolved into Ra, Rb, and the junctional resistance, Rj, by four different methods: cable analysis, resistance of uncoupled cells, measurements of pairs of (Rt, alpha) values in the same bladder at different transport rates, and the relation between Rt and Isc and between alpha and Isc. Rj proves to be effectively infinite (nominally 300 k omega muF) and independent of Isc, and Ra decreases from 154 to 4 omega muF with increasing Isc. In the resulting model of Na+ transport in \"tight\" epithelia, the apical membrane contains an amiloride-inhibited and Ca++-inhibited conductance pathway for Na+ entry; the basolateral membrane contains a Na+--K+-activated ATPase that extrudes Na+; intracellular (Na+) may exert negative feedback on apical membrane conductance; and aldosterone acts to stimulate Na+ entry at the apical membrane via the amiloride-sensitive pathway.", "PMID": 966267}
{"id": "pubmed23n0032_6667", "title": "Diffusion and 1/f noise.", "content": "The noise associated with ion transport through porous membranes is considered as a diffusion process. This is confirmed experimentally by measuring the noise spectra associated with pores of known dimension. It is then shown that one dimensional diffusion through pores of variable length can produce approximate 1/f noise spectra, if the distribution of lengths is proportional to (length)-1.", "contents": "Diffusion and 1/f noise. The noise associated with ion transport through porous membranes is considered as a diffusion process. This is confirmed experimentally by measuring the noise spectra associated with pores of known dimension. It is then shown that one dimensional diffusion through pores of variable length can produce approximate 1/f noise spectra, if the distribution of lengths is proportional to (length)-1.", "PMID": 966269}
{"id": "pubmed23n0032_6668", "title": "Permeability of a cell junction during intracellular injection of divalent cations.", "content": "Divalent cations are microinjected into Chironomus salivary gland cells while the cell-to-cell passage of fluorescein (330 dalton) and electrical coupling are monitored. Injections of Ca and Mg that substantially depolarize the cells produce block or marked slowing fluorescein passage, accompanied by electrical uncoupling. Injections of Ca, Mg or Sr that cause little depolarization, and presumably smaller elevation of divalent cation concentration in the cytoplasm, produce block or marked slowing of fluorescein passage with little or no detectable electrical uncoupling. This partial uncoupling may reflect total closure of a fraction of the channels in junctional membrane or partial closure of all channels.", "contents": "Permeability of a cell junction during intracellular injection of divalent cations. Divalent cations are microinjected into Chironomus salivary gland cells while the cell-to-cell passage of fluorescein (330 dalton) and electrical coupling are monitored. Injections of Ca and Mg that substantially depolarize the cells produce block or marked slowing fluorescein passage, accompanied by electrical uncoupling. Injections of Ca, Mg or Sr that cause little depolarization, and presumably smaller elevation of divalent cation concentration in the cytoplasm, produce block or marked slowing of fluorescein passage with little or no detectable electrical uncoupling. This partial uncoupling may reflect total closure of a fraction of the channels in junctional membrane or partial closure of all channels.", "PMID": 966268}
{"id": "pubmed23n0032_6669", "title": "Potassium movement during hyperpolarization of cardiac muscle.", "content": "When a bundle of cardiac muscle cells is hyperpolarized, membrane current declines with time. Voltage clamp experiments on sheep and cat ventricular bundles showed that the magnitude of inward current depended on the external K+ concentration. Following prolonged hyperpolarization, membrane current near the resting potential was generally outward. The half-time of decay of this outward current was approximately 2.5 sec at -60mV. The potential measured in the absence of externally supplied current was generally more negative than it would have been without conditioning hyperpolarization. The half-time of recovery of the current response following hyperpolarization was also approximately 2.5 sec at -60 mV, a factor of approximately 3.7 slower than the preceding decline of inward current. The rate of recovery has only a slight temperature dependence (Q10 approximately equal to 1.2). The experimental results are consistent with the idea that during hyperpolarization K+ is depleted from approximately 3% of the total muscle volume, and that the replenishment of K+ occurs primarily by K+ diffusion from a much larger fraction of the extracellular space.", "contents": "Potassium movement during hyperpolarization of cardiac muscle. When a bundle of cardiac muscle cells is hyperpolarized, membrane current declines with time. Voltage clamp experiments on sheep and cat ventricular bundles showed that the magnitude of inward current depended on the external K+ concentration. Following prolonged hyperpolarization, membrane current near the resting potential was generally outward. The half-time of decay of this outward current was approximately 2.5 sec at -60mV. The potential measured in the absence of externally supplied current was generally more negative than it would have been without conditioning hyperpolarization. The half-time of recovery of the current response following hyperpolarization was also approximately 2.5 sec at -60 mV, a factor of approximately 3.7 slower than the preceding decline of inward current. The rate of recovery has only a slight temperature dependence (Q10 approximately equal to 1.2). The experimental results are consistent with the idea that during hyperpolarization K+ is depleted from approximately 3% of the total muscle volume, and that the replenishment of K+ occurs primarily by K+ diffusion from a much larger fraction of the extracellular space.", "PMID": 966270}
{"id": "pubmed23n0032_6670", "title": "Mutants with reduced Ca activation in Paramecium aurelia.", "content": "Two heat-sensitive \"pawn\" mutants of Paramecium aurelia are capable of avoiding reactions when grown at 23 degrees C but not at 35 degrees C. Electrophysiological analyses show that Ca activation is reduces in the mutants even when they are grown at 23 degrees C. The maximal rate of rise and the peak of the evoked action potential (Ca-spike) in the mutants are smaller than those of wild type in a K-solution. After suppression of K conductance by either TEA+ or Ba++, the action potentials of the mutants peak at the same level as that of wild type. However, the maximal rate of rise of the mutants remains only about half that of wild type. Thus, the mutations affect Ca activation but not K activation. Incubation at a high temperature (35 degrees C) further reduces Ca activation to almost zero in the mutants but has little or no effect on wild type. This almost complete loss of Ca activation explains the lack of avoiding reactions when the mutants are grown at high temperatures. A double mutant containing two heat-sensitive mutations shows extremely reduced Ca activation even when grown at 23 degrees C.", "contents": "Mutants with reduced Ca activation in Paramecium aurelia. Two heat-sensitive \"pawn\" mutants of Paramecium aurelia are capable of avoiding reactions when grown at 23 degrees C but not at 35 degrees C. Electrophysiological analyses show that Ca activation is reduces in the mutants even when they are grown at 23 degrees C. The maximal rate of rise and the peak of the evoked action potential (Ca-spike) in the mutants are smaller than those of wild type in a K-solution. After suppression of K conductance by either TEA+ or Ba++, the action potentials of the mutants peak at the same level as that of wild type. However, the maximal rate of rise of the mutants remains only about half that of wild type. Thus, the mutations affect Ca activation but not K activation. Incubation at a high temperature (35 degrees C) further reduces Ca activation to almost zero in the mutants but has little or no effect on wild type. This almost complete loss of Ca activation explains the lack of avoiding reactions when the mutants are grown at high temperatures. A double mutant containing two heat-sensitive mutations shows extremely reduced Ca activation even when grown at 23 degrees C.", "PMID": 966271}
{"id": "pubmed23n0032_6671", "title": "Effects of continuous light and darkness on the eyes of the troglobitic salamander Typhlotriton spelaeus.", "content": "Larval Typhlotriton spelaeus collected from five caves in Pulaski Co., Missouri, were kept as larvae or induced to transform in darkness or continuous fluorescent illumination. Larvae maintained in darkness for 215 and 279 days had smaller eyes, smaller rod inner and outer segments, and fewer metaphase figures in the germinative zone of the neural retina than comparable larvae maintained in light (258 lux). Except for visual cell size, differences were small and for each characteristic exceptions were observed. One larva kept in light showed early retinal degeneration comparable to that in transformed adults to T. spelaeus. All larvae exhibited optomotor behavior both before and after the experiment. Among animals induced to transform by L-thyroxin and maintained in darkness 111 to 366 days, visual cell and pigment epithelium degeneration was more extensive and more frequent than in animals kept for the same length of time in light (237-298 lux). In darkness the frequency of animals with retinal degeneration increased between 111 and 366 days. In light some animals exhibited pigment epithelium reduction with normal visual cells, and others had free, pigmented cells in the subretinal space. These effects were not comparable to degeneration in darkness. Eyelids covered the eyes of only a few animals in both light and dark treatments. The extent of eyelid encroachment over the eye greater in darkness than in light. Most animals exhibited optomotor responses after experiments, but responses of animals kept in darkness were impaired in comparison to those of animals kept in light.", "contents": "Effects of continuous light and darkness on the eyes of the troglobitic salamander Typhlotriton spelaeus. Larval Typhlotriton spelaeus collected from five caves in Pulaski Co., Missouri, were kept as larvae or induced to transform in darkness or continuous fluorescent illumination. Larvae maintained in darkness for 215 and 279 days had smaller eyes, smaller rod inner and outer segments, and fewer metaphase figures in the germinative zone of the neural retina than comparable larvae maintained in light (258 lux). Except for visual cell size, differences were small and for each characteristic exceptions were observed. One larva kept in light showed early retinal degeneration comparable to that in transformed adults to T. spelaeus. All larvae exhibited optomotor behavior both before and after the experiment. Among animals induced to transform by L-thyroxin and maintained in darkness 111 to 366 days, visual cell and pigment epithelium degeneration was more extensive and more frequent than in animals kept for the same length of time in light (237-298 lux). In darkness the frequency of animals with retinal degeneration increased between 111 and 366 days. In light some animals exhibited pigment epithelium reduction with normal visual cells, and others had free, pigmented cells in the subretinal space. These effects were not comparable to degeneration in darkness. Eyelids covered the eyes of only a few animals in both light and dark treatments. The extent of eyelid encroachment over the eye greater in darkness than in light. Most animals exhibited optomotor responses after experiments, but responses of animals kept in darkness were impaired in comparison to those of animals kept in light.", "PMID": 966284}
{"id": "pubmed23n0032_6672", "title": "A staging system for forelimb regeneration in the axolotl, Ambystoma mexicanum.", "content": "A staging system has been devised for normal regeneration from the upper arm in the mature axolotl. It consists of seven externally definable stages: (1) Wound healing (WH): (2) Dedifferentiation (DD); (3) Early bud (EB); (4) Medium bud (MB); (5) Late bud (LB); (6) Palette (Pal), and (7) Digital outgrowth (DO). Serial histological sections of 38 regenerating limbs were used to correlate gross stages with microscopic events in the regenerative process.", "contents": "A staging system for forelimb regeneration in the axolotl, Ambystoma mexicanum. A staging system has been devised for normal regeneration from the upper arm in the mature axolotl. It consists of seven externally definable stages: (1) Wound healing (WH): (2) Dedifferentiation (DD); (3) Early bud (EB); (4) Medium bud (MB); (5) Late bud (LB); (6) Palette (Pal), and (7) Digital outgrowth (DO). Serial histological sections of 38 regenerating limbs were used to correlate gross stages with microscopic events in the regenerative process.", "PMID": 966285}
{"id": "pubmed23n0032_6673", "title": "Cytological observations of the ovarian epithelium in mammals during the reproductive cycle.", "content": "We have studied the ovarian epithelijm at various stages of the reproductive cycle in a number of mammalian species utilizing light microscopy, scanning microscopy, the freeze-fracture technique, transmission microscopy and by employing specialized tracers that use lanthanum and horseradish peroxidase. We found that the epithelial cells are joined by incomplete tight junctions, gap junctions, and desmosomes. The cytoplasmic matrix contains a large irregularly shaped nucleus, few microtubules, microfilaments, mitochondria, endoplasmic reticulum and a host of coated and non-coated vesicles of varying diameters. The saccules comprising the large Golgi complex and its companion vesicles are associated with a basal body-centriole complex: some of these saccules and affiliated vesicles are acid phosphatase positive. Surface modifications of ovarian epithelial cells include numerous microvilli, some of which have a bulbous tip, and plications of the lateral plasma membrane which are thought to accomodate volume changes of the ovary during follicular development. Many coated and non-coated endocytotic caveolae were found on these cells, particularly in the basal area. These caveolae internalized exogeneously administered horseradish peroxidase. We view the marked endocytotic activity as an efficient transport mechanism for partially removing substances from the interstitium of the ovary and the peritoneum.", "contents": "Cytological observations of the ovarian epithelium in mammals during the reproductive cycle. We have studied the ovarian epithelijm at various stages of the reproductive cycle in a number of mammalian species utilizing light microscopy, scanning microscopy, the freeze-fracture technique, transmission microscopy and by employing specialized tracers that use lanthanum and horseradish peroxidase. We found that the epithelial cells are joined by incomplete tight junctions, gap junctions, and desmosomes. The cytoplasmic matrix contains a large irregularly shaped nucleus, few microtubules, microfilaments, mitochondria, endoplasmic reticulum and a host of coated and non-coated vesicles of varying diameters. The saccules comprising the large Golgi complex and its companion vesicles are associated with a basal body-centriole complex: some of these saccules and affiliated vesicles are acid phosphatase positive. Surface modifications of ovarian epithelial cells include numerous microvilli, some of which have a bulbous tip, and plications of the lateral plasma membrane which are thought to accomodate volume changes of the ovary during follicular development. Many coated and non-coated endocytotic caveolae were found on these cells, particularly in the basal area. These caveolae internalized exogeneously administered horseradish peroxidase. We view the marked endocytotic activity as an efficient transport mechanism for partially removing substances from the interstitium of the ovary and the peritoneum.", "PMID": 966286}
{"id": "pubmed23n0032_6674", "title": "Ultrastructure of the secretory cells of the submucosal glands in the human maxillary sinus.", "content": "Tissue samples obtained from the lateral wall of the maxillary sinuses of five patients were examined by light microscopical, histochemical, and ultrastructural techniques. Submucosal glands were tubulo-alveolar mixed glands. The acini consisted of either all serous or all mucous cells, or a mixture of both. Serous granules were stained by toluidine blue, or by hematoxylin and eosin (H and E), but showed little or no reaction with periodic acid-Schiff (PAS) or Alcian blue. Mucous granules were pale in toluidine blue or H and E preparations, and consisted primarly of acid mucosubstances, as demonstrated by their staining reaction with PAS and Alcian blue. At the electron microscope level, the serous granules were either homogeneously dense, or showed a substructure consisting of at least two layers of distincly different electron-opacity. Typical mucous droplets consisted of a fibrillar network dispersed in a translucent matrix. A second secretory product was present in the mucous cells in the form of elongated, membrane-bounded structures containing numerous parallel filaments, which measured about 55 A in diameter. The mucous droplets and the filamentous bodies appear to arise from the opposite faces of the Golgi complex in the mucous cells. The filamentous bodies showed a pronounced tendency to fuse with the mucous droplets. All acini were surrounded by a well-defined myoepithelial layer and contained intercellular nerve terminals.", "contents": "Ultrastructure of the secretory cells of the submucosal glands in the human maxillary sinus. Tissue samples obtained from the lateral wall of the maxillary sinuses of five patients were examined by light microscopical, histochemical, and ultrastructural techniques. Submucosal glands were tubulo-alveolar mixed glands. The acini consisted of either all serous or all mucous cells, or a mixture of both. Serous granules were stained by toluidine blue, or by hematoxylin and eosin (H and E), but showed little or no reaction with periodic acid-Schiff (PAS) or Alcian blue. Mucous granules were pale in toluidine blue or H and E preparations, and consisted primarly of acid mucosubstances, as demonstrated by their staining reaction with PAS and Alcian blue. At the electron microscope level, the serous granules were either homogeneously dense, or showed a substructure consisting of at least two layers of distincly different electron-opacity. Typical mucous droplets consisted of a fibrillar network dispersed in a translucent matrix. A second secretory product was present in the mucous cells in the form of elongated, membrane-bounded structures containing numerous parallel filaments, which measured about 55 A in diameter. The mucous droplets and the filamentous bodies appear to arise from the opposite faces of the Golgi complex in the mucous cells. The filamentous bodies showed a pronounced tendency to fuse with the mucous droplets. All acini were surrounded by a well-defined myoepithelial layer and contained intercellular nerve terminals.", "PMID": 966287}
{"id": "pubmed23n0032_6675", "title": "Fine structure of the retinal epithelium and tapetum lucidum of the opossum (Didelphis virginiana).", "content": "The fine structure of the retinal epithelium has been studied by electron microscopy in the opossum (Didelphis virginiana). The retinal epithelium, over most of the retina, is typical of that in other vertebrates and consists of a single layer of heavily pigmented, cuboidal cells. These cells display extensive basal (scleral) infoldings and numerous apical (vitreal) processes which enclose photoreceptor outer segments. A semicircular area of the retinal epithelium in the superior fundus is further specialized as a tapetum lucidum. The reflecting material consists of a large quantity of lipoidal spheres scattered throughout the epithelial cells. Centrally in the tapetal area very few or no melanosomes are found, indicating a non-occlusible tapetum. Peripherally in be tapetum, the epithelial cells contain both reflecting material and melanosomes. As in the non-tapetal area, the epithelial cells of the tapetum display large amounts of smooth endoplasmic reticulum and numerous mitochondria. Bruch's membrane everywhere displays the usual pentalaminate structure described for most vertebrates. The choriocapillaris is also typical, in that numerous fenestrations are present in the endothelium bordering Bruch's membrane.", "contents": "Fine structure of the retinal epithelium and tapetum lucidum of the opossum (Didelphis virginiana). The fine structure of the retinal epithelium has been studied by electron microscopy in the opossum (Didelphis virginiana). The retinal epithelium, over most of the retina, is typical of that in other vertebrates and consists of a single layer of heavily pigmented, cuboidal cells. These cells display extensive basal (scleral) infoldings and numerous apical (vitreal) processes which enclose photoreceptor outer segments. A semicircular area of the retinal epithelium in the superior fundus is further specialized as a tapetum lucidum. The reflecting material consists of a large quantity of lipoidal spheres scattered throughout the epithelial cells. Centrally in the tapetal area very few or no melanosomes are found, indicating a non-occlusible tapetum. Peripherally in be tapetum, the epithelial cells contain both reflecting material and melanosomes. As in the non-tapetal area, the epithelial cells of the tapetum display large amounts of smooth endoplasmic reticulum and numerous mitochondria. Bruch's membrane everywhere displays the usual pentalaminate structure described for most vertebrates. The choriocapillaris is also typical, in that numerous fenestrations are present in the endothelium bordering Bruch's membrane.", "PMID": 966288}
{"id": "pubmed23n0032_6676", "title": "The structure of the hypothalamic inferior lobes of the blacktip reef shark: scanning and transmission electron microscopic observations.", "content": "The inferior lobes of the shark hypothalamus were examined with light, transmission and scanning electron microscopy. The cells bordering the floor of the lateral recess appear to be typical liquor-contacting neurons. With scanning electron microscopy (SEM) the apical ends of these cells are seen to bulge into the ventricular lumen. In contrast, the roof is lined by a more typical ependymal cell characterized by numerous cilia and microvilli. In addition, SEM reveals several kinds of supraependymal cells with processes that appear to penetrate the ventricular lining. A periventricular nucleus underlies the ependymal cells. Neurons of the periventricular nucleus contain numerous lipofuchsin granules. The rest of the inferior lobe consists of many neuronal fibers. The morphology of the hypothalamic inferior lobe is discussed in relation to its possible role in feeding and aggressive behavior in both elasmobranchs and teleosts.", "contents": "The structure of the hypothalamic inferior lobes of the blacktip reef shark: scanning and transmission electron microscopic observations. The inferior lobes of the shark hypothalamus were examined with light, transmission and scanning electron microscopy. The cells bordering the floor of the lateral recess appear to be typical liquor-contacting neurons. With scanning electron microscopy (SEM) the apical ends of these cells are seen to bulge into the ventricular lumen. In contrast, the roof is lined by a more typical ependymal cell characterized by numerous cilia and microvilli. In addition, SEM reveals several kinds of supraependymal cells with processes that appear to penetrate the ventricular lining. A periventricular nucleus underlies the ependymal cells. Neurons of the periventricular nucleus contain numerous lipofuchsin granules. The rest of the inferior lobe consists of many neuronal fibers. The morphology of the hypothalamic inferior lobe is discussed in relation to its possible role in feeding and aggressive behavior in both elasmobranchs and teleosts.", "PMID": 966289}
{"id": "pubmed23n0032_6677", "title": "Self complementarity in messenger RNA of collagen. I. Possible hairpin structures in regions coding for oligopeptides of glycine, proline (hydroxyproline) and alanine.", "content": "The periodic protein collagen is of special interest for the study of the relationship which exists between the structure of a protein and that of its mRNA, because oligopeptides containing glycine, proline (hydroxyproline) and alanine occur with great frequency in it. Collagen is particularly rich in these amino acids, which have codons containing only G and/or C in the obligatory first and second positions. If unlimited choice of codons for all amino acids were to occur, the stretch of mRNA coding for an alpha-chain should contain about 40% G and 31% C (Bachra et al., 1974). These high values suggest that a considerable degree of secondary structure will occur, unless selective codon use would result in the avoidance of G and C in optional third codon positions. In the present paper putative secondary structure formation in collagen mRNA was studied. This was done by studying the positions and frequencies of hairpin structures which could contain stem sections composed of the coding triplets of the above mentioned amino acids and hairpin sections of 4-40 bases. Calculation of the free energy contributions of such hairpin structures, using published values for the contributions of base-pair stacking, hairpin, bulge and interior loops and also taking into account the possible minimum number of base-pairs required for helix nucleation from a single-strand RNA (3 adjacent AU-pairs or 1 or 2 adjacent GC-pairs) led to the following conclusions. A considerable number of alternative, mutually exclusive hairpins can be constructed.", "contents": "Self complementarity in messenger RNA of collagen. I. Possible hairpin structures in regions coding for oligopeptides of glycine, proline (hydroxyproline) and alanine. The periodic protein collagen is of special interest for the study of the relationship which exists between the structure of a protein and that of its mRNA, because oligopeptides containing glycine, proline (hydroxyproline) and alanine occur with great frequency in it. Collagen is particularly rich in these amino acids, which have codons containing only G and/or C in the obligatory first and second positions. If unlimited choice of codons for all amino acids were to occur, the stretch of mRNA coding for an alpha-chain should contain about 40% G and 31% C (Bachra et al., 1974). These high values suggest that a considerable degree of secondary structure will occur, unless selective codon use would result in the avoidance of G and C in optional third codon positions. In the present paper putative secondary structure formation in collagen mRNA was studied. This was done by studying the positions and frequencies of hairpin structures which could contain stem sections composed of the coding triplets of the above mentioned amino acids and hairpin sections of 4-40 bases. Calculation of the free energy contributions of such hairpin structures, using published values for the contributions of base-pair stacking, hairpin, bulge and interior loops and also taking into account the possible minimum number of base-pairs required for helix nucleation from a single-strand RNA (3 adjacent AU-pairs or 1 or 2 adjacent GC-pairs) led to the following conclusions. A considerable number of alternative, mutually exclusive hairpins can be constructed.", "PMID": 966290}
{"id": "pubmed23n0032_6678", "title": "Elemental abundance as a factor in the origins of mineral nutrient requirements.", "content": "No element is found to be commonly required if it has an abundance of less than about 2 nM in the ocean, 20 mumoles/kg in the earth's crust, or 200 mumoles/100 moles Si in the cosmos. More than 40 elements are above these limits, but only 18 of them are commonly required (6 of these being dispensed with by some organism). It is postulated that all of the required elements fall under one of four hypotheses: H-I--a unique requirement dating from the origin of life; H-II--a unique requirement, acquired later; H-III--a primordial requirement which was satisfied by a number of elements, evolutionary adaptation being made to the most abundant member; H-IV--same as III, but a later acquisition. It is suggested that H, K (vs. Na), Mg (vs. Ca), C, N, O, P, S and Fe fall under H-I. Special requirements such as for B, Se and I fall under H-II. In H-III are K vs. Rb, Mg vs. Be(?), S vs. Se, Clvs. Br, H vs F(?), and Zn and Mn vs. various metals. In H-IV probably fall Ca vs. Sr, Na vs. Li (?), Mo vs. V, and Si vs. Ge. The most abundant heavy metal in the ocean is Zn, which may account for its utilization; other required heavy metals have special utility as electron carriers.", "contents": "Elemental abundance as a factor in the origins of mineral nutrient requirements. No element is found to be commonly required if it has an abundance of less than about 2 nM in the ocean, 20 mumoles/kg in the earth's crust, or 200 mumoles/100 moles Si in the cosmos. More than 40 elements are above these limits, but only 18 of them are commonly required (6 of these being dispensed with by some organism). It is postulated that all of the required elements fall under one of four hypotheses: H-I--a unique requirement dating from the origin of life; H-II--a unique requirement, acquired later; H-III--a primordial requirement which was satisfied by a number of elements, evolutionary adaptation being made to the most abundant member; H-IV--same as III, but a later acquisition. It is suggested that H, K (vs. Na), Mg (vs. Ca), C, N, O, P, S and Fe fall under H-I. Special requirements such as for B, Se and I fall under H-II. In H-III are K vs. Rb, Mg vs. Be(?), S vs. Se, Clvs. Br, H vs F(?), and Zn and Mn vs. various metals. In H-IV probably fall Ca vs. Sr, Na vs. Li (?), Mo vs. V, and Si vs. Ge. The most abundant heavy metal in the ocean is Zn, which may account for its utilization; other required heavy metals have special utility as electron carriers.", "PMID": 966291}
{"id": "pubmed23n0032_6679", "title": "Criteria for optimising phylogenetic trees and the problem of determining the root of a tree.", "content": "The process of determining the optimal phylogenetic tree from amino acid sequences or comparable data is divided into six stages. Particular attention is given both to the criteria that are used when testing for the optimal tree and the problem of determining the position of the original ancestor. Four types of criteria for evaluating the optimal tree are considered: 1. parsimony (fewest total changes), 2. path lengths from an ancestor to existing species, 3. subtracting the difference between each pair of species as measured on the tree and as compared directly with the data (\"excess differences\"), 4. Moore Residual Coefficient. These criteria are examined on a set of test data and some of the reasons for the differences among them are discussed. For example, the \"average percent standard deviation\" weights excess differences unequally in inverse proportion to the square of the observed differences. The Moore Residual Coefficient and the \"excess differences\" will not necessarily give a value of zero when there are no duplicated changes unless there can only be two states for each character (i.e. binary data). The path length and difference criteria (as well as the Moore Residual Coefficient) give unequal weighting to the individual branches of the tree by counting some branches more times than others. Particularly because of this some criteria will reject trees that are equally parsimonious and the criteria are said to be invalid. However the criterion of parsimony is insensitive in that it can give the same value for several basic networks and it does not specify the position of the original ancestor, the root of the tree. The importance is emphasised of stating a model and examining its predictions before a criterion is chosen to select the best network. The number of rooted trees that can be derived from a basic network (or unrooted tree) is described in relation to how detailed a description of the original ancestor is required. Four methods are described for determining the position of the root of the tree or original ancestor. Each method depends upon some additional information to that used in constructing the basic network and the method chosen will depend on this additional knowledge.", "contents": "Criteria for optimising phylogenetic trees and the problem of determining the root of a tree. The process of determining the optimal phylogenetic tree from amino acid sequences or comparable data is divided into six stages. Particular attention is given both to the criteria that are used when testing for the optimal tree and the problem of determining the position of the original ancestor. Four types of criteria for evaluating the optimal tree are considered: 1. parsimony (fewest total changes), 2. path lengths from an ancestor to existing species, 3. subtracting the difference between each pair of species as measured on the tree and as compared directly with the data (\"excess differences\"), 4. Moore Residual Coefficient. These criteria are examined on a set of test data and some of the reasons for the differences among them are discussed. For example, the \"average percent standard deviation\" weights excess differences unequally in inverse proportion to the square of the observed differences. The Moore Residual Coefficient and the \"excess differences\" will not necessarily give a value of zero when there are no duplicated changes unless there can only be two states for each character (i.e. binary data). The path length and difference criteria (as well as the Moore Residual Coefficient) give unequal weighting to the individual branches of the tree by counting some branches more times than others. Particularly because of this some criteria will reject trees that are equally parsimonious and the criteria are said to be invalid. However the criterion of parsimony is insensitive in that it can give the same value for several basic networks and it does not specify the position of the original ancestor, the root of the tree. The importance is emphasised of stating a model and examining its predictions before a criterion is chosen to select the best network. The number of rooted trees that can be derived from a basic network (or unrooted tree) is described in relation to how detailed a description of the original ancestor is required. Four methods are described for determining the position of the root of the tree or original ancestor. Each method depends upon some additional information to that used in constructing the basic network and the method chosen will depend on this additional knowledge.", "PMID": 966292}
{"id": "pubmed23n0032_6680", "title": "Arsenic and cancer: the still unanswered question.", "content": "For some 2,500 years, arsenic was considered an important element of pharmacopeias and was praised for its medicinal qualities. However, an incidental link with cancer was suggested during the nineteenth century, and since that time a controversy has ensued with regard to the compound's tumorigenicity. Several epidemiological surveys, for example, have suggested that arsenic induces lung, liver, or skin cancer in humans. On the other hand, all experimental attempts to reproduce such neoplasms in laboratory animals have consistently failed, thereby denying support to the human data. Recently arsenic was reported to be mutagenic. However, because of the inconsistency of most mutagenesis findings at present, this also cannot be regarded as supporting evidence. Furthermore, a recent trend has been to consider arsenic as beneficial in cancer prevention and in maintaining the health of farm animals, and, perhaps, humans. A critical review of epidemiological and experimental data from the literature has been made in an attempt to present an objective picture of this controversial and sensitive question and to encourage further research, which may ultimately determine whether arsenic deserves its execrable reputation.", "contents": "Arsenic and cancer: the still unanswered question. For some 2,500 years, arsenic was considered an important element of pharmacopeias and was praised for its medicinal qualities. However, an incidental link with cancer was suggested during the nineteenth century, and since that time a controversy has ensued with regard to the compound's tumorigenicity. Several epidemiological surveys, for example, have suggested that arsenic induces lung, liver, or skin cancer in humans. On the other hand, all experimental attempts to reproduce such neoplasms in laboratory animals have consistently failed, thereby denying support to the human data. Recently arsenic was reported to be mutagenic. However, because of the inconsistency of most mutagenesis findings at present, this also cannot be regarded as supporting evidence. Furthermore, a recent trend has been to consider arsenic as beneficial in cancer prevention and in maintaining the health of farm animals, and, perhaps, humans. A critical review of epidemiological and experimental data from the literature has been made in an attempt to present an objective picture of this controversial and sensitive question and to encourage further research, which may ultimately determine whether arsenic deserves its execrable reputation.", "PMID": 966311}
{"id": "pubmed23n0032_6681", "title": "Effect of acute carbon monoxide poisoning on serum lactate dehydrogenase and creatine phosphokinase.", "content": "Young adult rats were exposed to 1,500 ppm CO in air for 2 hr. Blood carboxyhemoglobin (COHb) concentration immediately postexposure was 55.68 +/- 0.90%. Serum lactate dehydrogenase (LDH) activity peaked at 117% above control 2 hr postexposure, while serum creatine phosphokinase (CPK) activity was maximal at 132% above control immediately following termination of exposure. Serum LDH activity declined to control within 8 hr, while serum CPK undershot controls at 8 hr and returned to the control value by 24 hr.", "contents": "Effect of acute carbon monoxide poisoning on serum lactate dehydrogenase and creatine phosphokinase. Young adult rats were exposed to 1,500 ppm CO in air for 2 hr. Blood carboxyhemoglobin (COHb) concentration immediately postexposure was 55.68 +/- 0.90%. Serum lactate dehydrogenase (LDH) activity peaked at 117% above control 2 hr postexposure, while serum creatine phosphokinase (CPK) activity was maximal at 132% above control immediately following termination of exposure. Serum LDH activity declined to control within 8 hr, while serum CPK undershot controls at 8 hr and returned to the control value by 24 hr.", "PMID": 966312}
{"id": "pubmed23n0032_6682", "title": "A study of chromosomes of schistosomiasis patients under oxaminiquine (UK 4271) treatment.", "content": "Blood samples from 24 patients infested with Schistosoma mansoni were drawn immediately before and 2 days after the administration of a single therapeutic dose of 12-15 mg/kg oral oxaminiquine. Two-day lymphocyte cultures were obtained and about 100 mitoses from each blood sample were analyzed for chromosome aberrations. No significant differences were observed between the \"before\" and \"after\" cultures in the frequencies of aberrations resulting from spindle, chromatidic, or chromosome events. It is concluded that there is no reason to fear harmful effects on the chromosomes of patients from treatment with oxaminiquine.", "contents": "A study of chromosomes of schistosomiasis patients under oxaminiquine (UK 4271) treatment. Blood samples from 24 patients infested with Schistosoma mansoni were drawn immediately before and 2 days after the administration of a single therapeutic dose of 12-15 mg/kg oral oxaminiquine. Two-day lymphocyte cultures were obtained and about 100 mitoses from each blood sample were analyzed for chromosome aberrations. No significant differences were observed between the \"before\" and \"after\" cultures in the frequencies of aberrations resulting from spindle, chromatidic, or chromosome events. It is concluded that there is no reason to fear harmful effects on the chromosomes of patients from treatment with oxaminiquine.", "PMID": 966313}
{"id": "pubmed23n0032_6683", "title": "Teratology and percutaneous toxicity studies on hair dyes.", "content": "Twelve hair dye formulations were tested for systemic toxicity by topical application twice weekly for 13 wk to groups of 12 New Zealand white rabbits and for teratologic effects following applications to groups of 20 pregnant Charles River CD rats on days 1, 4, 7, 10, 13, 16, and 19 of gestation. The three semipermanent formulations were applied as is, and the nine oxidation dyes were mixed 1:1 with 6% hydrogen peroxide just prior to application, as in normal use. The formulations induced a broad spectrum of dyes and dye intermediates used or considered useful in oxidative and semipermanent hair color products. In the teratology study no biologically significant soft tissue or skeletal changes were noted. Similarly, the mean numbers of corpora lutea, implantation sites, live fetuses, and resorptions per pregnancy, as well as numbers of litters with resporptions, were not significantly affected by the dye treatment. In the percutaneous toxicity study there was no evidence of compound-induced systemic effects. Microscopic examination of 25 tissues from each animal gave no indication of histomorphologic evidence of toxicity. No dye discoloration of urine was seen at any time during the test or at necropsy. Some of the dye groups showed epidermal hyperplasia, which was probably a reflection of slight irritation due to the frequency of application of the oxidation formulations.", "contents": "Teratology and percutaneous toxicity studies on hair dyes. Twelve hair dye formulations were tested for systemic toxicity by topical application twice weekly for 13 wk to groups of 12 New Zealand white rabbits and for teratologic effects following applications to groups of 20 pregnant Charles River CD rats on days 1, 4, 7, 10, 13, 16, and 19 of gestation. The three semipermanent formulations were applied as is, and the nine oxidation dyes were mixed 1:1 with 6% hydrogen peroxide just prior to application, as in normal use. The formulations induced a broad spectrum of dyes and dye intermediates used or considered useful in oxidative and semipermanent hair color products. In the teratology study no biologically significant soft tissue or skeletal changes were noted. Similarly, the mean numbers of corpora lutea, implantation sites, live fetuses, and resorptions per pregnancy, as well as numbers of litters with resporptions, were not significantly affected by the dye treatment. In the percutaneous toxicity study there was no evidence of compound-induced systemic effects. Microscopic examination of 25 tissues from each animal gave no indication of histomorphologic evidence of toxicity. No dye discoloration of urine was seen at any time during the test or at necropsy. Some of the dye groups showed epidermal hyperplasia, which was probably a reflection of slight irritation due to the frequency of application of the oxidation formulations.", "PMID": 966314}
{"id": "pubmed23n0032_6684", "title": "Strain differences in histopathologic, hematologic, and blood chemistry changes induced in mice by a technical and a purified preparation of 2,4,5-trichlorphenoxyacetic acid.", "content": "Including controls, 978 mice were studied. On days corresponding to days 6 through 14 of pregnancy, groups of pregnant and nonpregnant CD-1 mice and male and nonpregnant female dihybrid cross F2 mice received by gavage 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) ranging in dosage from 30 to 140 mg/kg. Some groups received a technical preparation containing 97.9 +/- 0.4% 2,4,5-T and some a purified preparation containing 99 +/- 0.3% 2,4,5-T. Mice were sacrificed when they became moribund and at 1, 2, 4, 6, 8, and 11 days after beginning treatment. Sick or moribund mice sacrificed after 2-9 doses of 2,4,5-T often showed severe myocardial lesions, hypocellularlity of the bone marrow, and depletion of lymphocytes in the thymus, spleen or lymph nodes. They also showed marked hematologic and blood chemistry changes. Treated mice remaining healthy showed few or no lesions or blood chemistry changes, but often developed a mild anemia attributable to a hemolytic effect of 2,4,5-T. The incidence of animals becoming moribund was less than 1% in the CD-1 mice, including those given 140 mg/kg, and 53-82% in groups of male and female F2 mice receiving 120 mg/kg 2,4,5-T. The incidence of moribund mice tended to be higher in male than in female F2 mice and in those given the purified compound. These findings indicate that impairment of maternal health by severe lesions early in gestation is not the primary cause of an increase in incidence of fetal abnormalities observed in mice given 2,4,5-t. they also indicate that the lesions are due primarily to 2,4,5-T, rather than contaminants in the technical preparation, and illustrate the importance of using more than one strain of mouse in a toxicologic or teratologic study.", "contents": "Strain differences in histopathologic, hematologic, and blood chemistry changes induced in mice by a technical and a purified preparation of 2,4,5-trichlorphenoxyacetic acid. Including controls, 978 mice were studied. On days corresponding to days 6 through 14 of pregnancy, groups of pregnant and nonpregnant CD-1 mice and male and nonpregnant female dihybrid cross F2 mice received by gavage 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) ranging in dosage from 30 to 140 mg/kg. Some groups received a technical preparation containing 97.9 +/- 0.4% 2,4,5-T and some a purified preparation containing 99 +/- 0.3% 2,4,5-T. Mice were sacrificed when they became moribund and at 1, 2, 4, 6, 8, and 11 days after beginning treatment. Sick or moribund mice sacrificed after 2-9 doses of 2,4,5-T often showed severe myocardial lesions, hypocellularlity of the bone marrow, and depletion of lymphocytes in the thymus, spleen or lymph nodes. They also showed marked hematologic and blood chemistry changes. Treated mice remaining healthy showed few or no lesions or blood chemistry changes, but often developed a mild anemia attributable to a hemolytic effect of 2,4,5-T. The incidence of animals becoming moribund was less than 1% in the CD-1 mice, including those given 140 mg/kg, and 53-82% in groups of male and female F2 mice receiving 120 mg/kg 2,4,5-T. The incidence of moribund mice tended to be higher in male than in female F2 mice and in those given the purified compound. These findings indicate that impairment of maternal health by severe lesions early in gestation is not the primary cause of an increase in incidence of fetal abnormalities observed in mice given 2,4,5-t. they also indicate that the lesions are due primarily to 2,4,5-T, rather than contaminants in the technical preparation, and illustrate the importance of using more than one strain of mouse in a toxicologic or teratologic study.", "PMID": 966315}
{"id": "pubmed23n0032_6685", "title": "Metabolism of 109Cd in rats fed normal and low-calcium diets.", "content": "Growing male rats were fed purified diets that contained either 0.6% or 0.1% calcium to investigate the relationship of calcium intake to the uptake, tissue distribution, and excretion of 109Cd. An equal number of rats were fed either the 0.6 or 0.1% calcium diets for 4 wk before they were used for experiments. In the first experiment 11 rats from each dietary group were administered 5 muCi 109Cd by stomach tube and were then maintained in metabolism cages for 72 hr. Animals fed the low-calcium diet took up more 109Cd, as significantly higher levels of radioactivity were found in the intestinal mucosa, serum, lungs, liver, kidneys, and urine and a significantly lower level was found in the feces. Higher levels of 109Cd, associated with low-molecular-weight proteins that may be related to the absorption process, were found in the intestinal mucosa of the low-calcium group. In the second experiment 10 rats from each dietary group were administered 5 muCi 109Cd by subcutaneous injection and then maintained in a metabolism cage for 72 hr. No significant differences were found in the distribution or excretion of 109Cd except for the lungs where radioactivity was greater in the low-calcium group. The results of the study indicate that the enhanced cadmium toxicity observed in calcium-deficient animals exposed to the heavy metal is the result of an increased uptake from the small intestine.", "contents": "Metabolism of 109Cd in rats fed normal and low-calcium diets. Growing male rats were fed purified diets that contained either 0.6% or 0.1% calcium to investigate the relationship of calcium intake to the uptake, tissue distribution, and excretion of 109Cd. An equal number of rats were fed either the 0.6 or 0.1% calcium diets for 4 wk before they were used for experiments. In the first experiment 11 rats from each dietary group were administered 5 muCi 109Cd by stomach tube and were then maintained in metabolism cages for 72 hr. Animals fed the low-calcium diet took up more 109Cd, as significantly higher levels of radioactivity were found in the intestinal mucosa, serum, lungs, liver, kidneys, and urine and a significantly lower level was found in the feces. Higher levels of 109Cd, associated with low-molecular-weight proteins that may be related to the absorption process, were found in the intestinal mucosa of the low-calcium group. In the second experiment 10 rats from each dietary group were administered 5 muCi 109Cd by subcutaneous injection and then maintained in a metabolism cage for 72 hr. No significant differences were found in the distribution or excretion of 109Cd except for the lungs where radioactivity was greater in the low-calcium group. The results of the study indicate that the enhanced cadmium toxicity observed in calcium-deficient animals exposed to the heavy metal is the result of an increased uptake from the small intestine.", "PMID": 966316}
{"id": "pubmed23n0032_6686", "title": "Effects of platinum and palladium salts on parameters of drug metabolism in rat liver.", "content": "The effects of various salts of platinum of palladium were determined on the parameters of the microsomal mixed-function oxidase system from rat liver. The intraperitoneal injection of PtCl4 or Pd(NO3)2 increased the hexobarbital-induced sleeping time in vivo and generally decreased the aminopyrine demethylase and the content of cytochrome P-450 in isolated hepatic microsomes. The dietary administration of various salts of platinum or palladium for 1 wk generally decreased or had no effect on the parameters of drug metabolism by isolated microsomes and after 4 or more wk generally had no effect on, or increased, the parameters. The addition of 0.15-0.2 mM PtCl4 or 0.2-0.3 mM Pd(NO3)2 to the incubation medium (containing 5 mM MgCl2) inhibited the aminopyrine demethylase of isolated hepatic microsomes by 50%.", "contents": "Effects of platinum and palladium salts on parameters of drug metabolism in rat liver. The effects of various salts of platinum of palladium were determined on the parameters of the microsomal mixed-function oxidase system from rat liver. The intraperitoneal injection of PtCl4 or Pd(NO3)2 increased the hexobarbital-induced sleeping time in vivo and generally decreased the aminopyrine demethylase and the content of cytochrome P-450 in isolated hepatic microsomes. The dietary administration of various salts of platinum or palladium for 1 wk generally decreased or had no effect on the parameters of drug metabolism by isolated microsomes and after 4 or more wk generally had no effect on, or increased, the parameters. The addition of 0.15-0.2 mM PtCl4 or 0.2-0.3 mM Pd(NO3)2 to the incubation medium (containing 5 mM MgCl2) inhibited the aminopyrine demethylase of isolated hepatic microsomes by 50%.", "PMID": 966317}
{"id": "pubmed23n0032_6687", "title": "Lupin alkaloids from teratogenic and nonteratogenic lupins. III. Identification of anagyrine as the probable teratogen by feeding trials.", "content": "Alkaloidal extracts from teratogenic lupins produced congenital deformities in calves typical of crooked calf disease when the extracts were administered to pregnant cows during the susceptible gestational period. These data and previous epidemiologic studies suggest that one of the four alkaloids in the preparation, anagyrine, is the responsible teratogen. Severity of the malformations was directly related to the level of anagyrine present in the preparations administered.", "contents": "Lupin alkaloids from teratogenic and nonteratogenic lupins. III. Identification of anagyrine as the probable teratogen by feeding trials. Alkaloidal extracts from teratogenic lupins produced congenital deformities in calves typical of crooked calf disease when the extracts were administered to pregnant cows during the susceptible gestational period. These data and previous epidemiologic studies suggest that one of the four alkaloids in the preparation, anagyrine, is the responsible teratogen. Severity of the malformations was directly related to the level of anagyrine present in the preparations administered.", "PMID": 966318}
{"id": "pubmed23n0032_6688", "title": "Lupin alkaloids from teratogenic and nonteratogenic lupins. IV. Concentration of total alkaloids, individual major alkaloids, and the teratogen anagyrine as a function of plant part and stage of growth and their relationship to crooked calf disease.", "content": "The concentrations of total alkaloids and individual major alkaloids including the teratogen anagyrine were measured in various plant parts of teratogenic lupins as the plants matured. All alkaloids including angyrine were high in concentration in above-ground parts early in growth and decreased as plants matured, except for an increase in mature, intact seeds. Seeds were highest, followed by early growth leaves and stems. Roots were lowest with mature leaves and stems only slightly higher. Pregnant cows have the greatest risk of giving birth to calves with crooked calf disease when the concentration of the teratogen anagyrine is highest and the cows are in the susceptible 40-75 day gestation period when ingesting the plant.", "contents": "Lupin alkaloids from teratogenic and nonteratogenic lupins. IV. Concentration of total alkaloids, individual major alkaloids, and the teratogen anagyrine as a function of plant part and stage of growth and their relationship to crooked calf disease. The concentrations of total alkaloids and individual major alkaloids including the teratogen anagyrine were measured in various plant parts of teratogenic lupins as the plants matured. All alkaloids including angyrine were high in concentration in above-ground parts early in growth and decreased as plants matured, except for an increase in mature, intact seeds. Seeds were highest, followed by early growth leaves and stems. Roots were lowest with mature leaves and stems only slightly higher. Pregnant cows have the greatest risk of giving birth to calves with crooked calf disease when the concentration of the teratogen anagyrine is highest and the cows are in the susceptible 40-75 day gestation period when ingesting the plant.", "PMID": 966319}
{"id": "pubmed23n0032_6689", "title": "Reproductive, teratologic, and mutagenic studies with some polydimethylsiloxanes.", "content": "The purpose of these studies was to evaluate the effects of selected polydimethylsiloxanes on reproduction and fetal development in rats and rabbits and to determine the mutagenic potential of one such material in mice. In two separate three-phase studies in rats and rabbits with a 350 centistoke medical grade fluid, the only significant effect noted was an apparent dose-related incidence of in utero mortality at dose levels of 200 and 1,000 mg/kg sc in rats in one study. No evidence of fetotoxicity was obtained in the second study at the same dose levels. The incidence of talipes varus at a sc dose level of 200 mg/kg in rabbits (8.8%) is at or above that expected for control populations. The nonoccurrence of talipes varus at the higher level of 1,000 mg/kg and the absence of this defect in the companion study casts doubt on the significance of this finding. A 7-cs pump fluid was nonteratogenic in rats at oral doses as high as 1,000 mg/kg and was nonmutagenic in ma1e mice at ip doses of 5 and 10 g/kg. A 10-cs fluid was nonteratogenic in rabbits at a dermal dose level of 200 mg/kg.", "contents": "Reproductive, teratologic, and mutagenic studies with some polydimethylsiloxanes. The purpose of these studies was to evaluate the effects of selected polydimethylsiloxanes on reproduction and fetal development in rats and rabbits and to determine the mutagenic potential of one such material in mice. In two separate three-phase studies in rats and rabbits with a 350 centistoke medical grade fluid, the only significant effect noted was an apparent dose-related incidence of in utero mortality at dose levels of 200 and 1,000 mg/kg sc in rats in one study. No evidence of fetotoxicity was obtained in the second study at the same dose levels. The incidence of talipes varus at a sc dose level of 200 mg/kg in rabbits (8.8%) is at or above that expected for control populations. The nonoccurrence of talipes varus at the higher level of 1,000 mg/kg and the absence of this defect in the companion study casts doubt on the significance of this finding. A 7-cs pump fluid was nonteratogenic in rats at oral doses as high as 1,000 mg/kg and was nonmutagenic in ma1e mice at ip doses of 5 and 10 g/kg. A 10-cs fluid was nonteratogenic in rabbits at a dermal dose level of 200 mg/kg.", "PMID": 966320}
{"id": "pubmed23n0032_6690", "title": "Sublethal effects of chronic lead ingestion in mallard ducks.", "content": "Mallard drakes (Anas platyrhynchos) fed 1, 5, or 25 ppm lead nitrate were bled and sacrificed at 3-wk intervals. No mortality occurred, and the pathologic lesions usually associated with lead poisoning were not found. Changes in hematocrit and hemoglobin concentration did not occur. After 3-wk ducks fed 25 ppm lead exhibited a 40% inhibition of blood delta-aminolevulinic acid dehydratase activity that persisted through 12 wk exposure. After 12 wk treatment similar enzyme inhibition was present in the ducks fed 5 ppm lead. At 3 wk there was a small accumulation of lead (less than 1 ppm) in the liver and kidneys of ducks fed 25ppm lead; no further increases occurred throughout the exposure. No significant accumulation of lead occurred the the tibiae or wing bones. Groups of ducks fed 5 and 25 ppm diets for 12 wk were placed on clean feed and examined through a 12 wk posttreatment period. After 3 wk on clean diet delta-aminolevulinic acid dehydratase activity and lead concentrations in the blood had returned to pretreatment levels. Even though lead concentrations in the blood, soft organs and bone were low, a highly significant negative correlation between blood lead and blood enzyme activity was obtained. This enzyme bioassay should provide a sensitive and precise estimate for monitoring lead in the blood for waterflow.", "contents": "Sublethal effects of chronic lead ingestion in mallard ducks. Mallard drakes (Anas platyrhynchos) fed 1, 5, or 25 ppm lead nitrate were bled and sacrificed at 3-wk intervals. No mortality occurred, and the pathologic lesions usually associated with lead poisoning were not found. Changes in hematocrit and hemoglobin concentration did not occur. After 3-wk ducks fed 25 ppm lead exhibited a 40% inhibition of blood delta-aminolevulinic acid dehydratase activity that persisted through 12 wk exposure. After 12 wk treatment similar enzyme inhibition was present in the ducks fed 5 ppm lead. At 3 wk there was a small accumulation of lead (less than 1 ppm) in the liver and kidneys of ducks fed 25ppm lead; no further increases occurred throughout the exposure. No significant accumulation of lead occurred the the tibiae or wing bones. Groups of ducks fed 5 and 25 ppm diets for 12 wk were placed on clean feed and examined through a 12 wk posttreatment period. After 3 wk on clean diet delta-aminolevulinic acid dehydratase activity and lead concentrations in the blood had returned to pretreatment levels. Even though lead concentrations in the blood, soft organs and bone were low, a highly significant negative correlation between blood lead and blood enzyme activity was obtained. This enzyme bioassay should provide a sensitive and precise estimate for monitoring lead in the blood for waterflow.", "PMID": 966321}
{"id": "pubmed23n0032_6691", "title": "Phytochemical and toxicological screening of household ornamental plants potentially toxic to humans. I.", "content": "Thirty different genera of household ornamental plants have been investigated to determine the loci and natures of potential toxins and any injurious effects produced in experimental animals. Different plant parts were tested for alkaloids, glycosides, steroids, saponins, and cyanogenic glycosides. At least seven genera showed detectable concentrations of alkaloids. Glycoside tests were variable, and there was an indication that several species may contain steroid components. Sixteen genera showed positive saponin foam tests. None of the species were clearly cyanogenic. Preliminary biologic screening on rats and mice is given.", "contents": "Phytochemical and toxicological screening of household ornamental plants potentially toxic to humans. I. Thirty different genera of household ornamental plants have been investigated to determine the loci and natures of potential toxins and any injurious effects produced in experimental animals. Different plant parts were tested for alkaloids, glycosides, steroids, saponins, and cyanogenic glycosides. At least seven genera showed detectable concentrations of alkaloids. Glycoside tests were variable, and there was an indication that several species may contain steroid components. Sixteen genera showed positive saponin foam tests. None of the species were clearly cyanogenic. Preliminary biologic screening on rats and mice is given.", "PMID": 966322}
{"id": "pubmed23n0032_6692", "title": "Heptachlor: toxicity to and uptake by several estuarine organisms.", "content": "Technical-grade heptachlor (65% heptachlor, 22% trans-chlordane, 2% cis-chlordane, and 2% nonachlor) was tested in 96-hr bioassays to determine its toxicity to estuarine animals. The test organisms and the 96-hr LC50 or EC50s based on measured concentrations in water) are as follows: American oyster (Crassostrea virginica), 1.5 mug/liter; pink shrimp (Penaeus duorarum), 0.11 mug/liter; grass shrimp (Palaemonetes vulgaris), 1.06 mug/liter; sheepshead minnow (Cyprinodon variegatus), 3.68 mug/liter; pinfish (Lagodon rhomboides), 3.77 mug/liter; and spot (Leiostomus xanthurus), 0.85 mug/liter. Analytical-grade heptachlor (99.8% heptachlor) and heptachlor epoxide (99%) were also studied. The analytical-grade heptachlor 96-hr LC50 for pink shrimp and spot was 0.03 mug/liter and 0.86 mug/liter, respectively, while that for pink shrimp exposed to heptachlor epoxide was 0.04 mug/liter. Heptachlor was accumulated and some metabolized to its epoxide by all animals tested. Fish and oysters accumulated heptachlor in their tissues 2,800-21,300 times the measured concentration in water; shrimp, only 200-700 times.", "contents": "Heptachlor: toxicity to and uptake by several estuarine organisms. Technical-grade heptachlor (65% heptachlor, 22% trans-chlordane, 2% cis-chlordane, and 2% nonachlor) was tested in 96-hr bioassays to determine its toxicity to estuarine animals. The test organisms and the 96-hr LC50 or EC50s based on measured concentrations in water) are as follows: American oyster (Crassostrea virginica), 1.5 mug/liter; pink shrimp (Penaeus duorarum), 0.11 mug/liter; grass shrimp (Palaemonetes vulgaris), 1.06 mug/liter; sheepshead minnow (Cyprinodon variegatus), 3.68 mug/liter; pinfish (Lagodon rhomboides), 3.77 mug/liter; and spot (Leiostomus xanthurus), 0.85 mug/liter. Analytical-grade heptachlor (99.8% heptachlor) and heptachlor epoxide (99%) were also studied. The analytical-grade heptachlor 96-hr LC50 for pink shrimp and spot was 0.03 mug/liter and 0.86 mug/liter, respectively, while that for pink shrimp exposed to heptachlor epoxide was 0.04 mug/liter. Heptachlor was accumulated and some metabolized to its epoxide by all animals tested. Fish and oysters accumulated heptachlor in their tissues 2,800-21,300 times the measured concentration in water; shrimp, only 200-700 times.", "PMID": 966323}
{"id": "pubmed23n0032_6693", "title": "Elution of benzo[alpha]pyrene from carbon particles in the respiratory tract of mice.", "content": "The effect of carrier particle size on the rate of dissociation of benzo[alpha]pyrene (BaP) from carrier particles deposited in the respiratory tract of mice was studied. BaP-coated carbon particles (in two size ranges, 0.5-1.0 and 15-30 mum) plus 103Ru-tagged carbon tracer particles were intratracheally instilled in mice. The clearance of carbon particles and the simultaneous rate of elimination of BaP from the respiratory tract was measured. BaP adsorbed to 15- to 30-muM carbon particles was eliminated from the lung at essentially the same rate as the carbon particles were cleared. In contrast, BaP adsorbed to 0.5- to 1.0-muM carbon particles was eliminated from the lung approximately 4 times faster than the carbon particles were cleared. The persistence of carcinogens and their rates of elution from carrier particles are discussed in relation to the pathogenesis of lung cancer in animals treated with carcinogen-carrier particle preparations.", "contents": "Elution of benzo[alpha]pyrene from carbon particles in the respiratory tract of mice. The effect of carrier particle size on the rate of dissociation of benzo[alpha]pyrene (BaP) from carrier particles deposited in the respiratory tract of mice was studied. BaP-coated carbon particles (in two size ranges, 0.5-1.0 and 15-30 mum) plus 103Ru-tagged carbon tracer particles were intratracheally instilled in mice. The clearance of carbon particles and the simultaneous rate of elimination of BaP from the respiratory tract was measured. BaP adsorbed to 15- to 30-muM carbon particles was eliminated from the lung at essentially the same rate as the carbon particles were cleared. In contrast, BaP adsorbed to 0.5- to 1.0-muM carbon particles was eliminated from the lung approximately 4 times faster than the carbon particles were cleared. The persistence of carcinogens and their rates of elution from carrier particles are discussed in relation to the pathogenesis of lung cancer in animals treated with carcinogen-carrier particle preparations.", "PMID": 966324}
{"id": "pubmed23n0032_6694", "title": "DNA damage produced by povidone-iodine in cultured human diploid cells.", "content": "Povidone-iodine is capable of selective altering the DNA of human diploid cells growing in culture. This finding extends to eukaryotic cells, the previously reported DNA-modifying activity of this agent for bacteria. In view of the known relationship between DNA-modifying activity and potential carcinogenicity, the results suggest that the potential hazards posed by the widespread use of this agent be evaluated.", "contents": "DNA damage produced by povidone-iodine in cultured human diploid cells. Povidone-iodine is capable of selective altering the DNA of human diploid cells growing in culture. This finding extends to eukaryotic cells, the previously reported DNA-modifying activity of this agent for bacteria. In view of the known relationship between DNA-modifying activity and potential carcinogenicity, the results suggest that the potential hazards posed by the widespread use of this agent be evaluated.", "PMID": 966325}
{"id": "pubmed23n0032_6695", "title": "Effect of mycotoxins on uptake and degradation of [125I] albumin in mouse liver and kidney lysosomes.", "content": "Some mycotoxins have been evaluated with respect to effects on the reticuloendothelial function of uptake and degradation of a soluble, denatured protein in phagolysosomes isolated from mouse liver and kidneys. Toxins were dissolved in dimethylsulfoxide and administered intraperitoneally. Particulate fractions isolated from liver and kidney homogenates were assayed for proteolytic activity within osmotically active particles (phagolysosomes). Treatment with citrinin, penitrem A, streigmatocystin, and zearalenone produced inhibition of proteolysis in kidney but not in liver phagolysosomes. Moniliformin and T-2 toxin had no detectable effect on lysosome formation or function in either tissue. The phagolysosomal toxicity of these compounds appeared to be more toward kidney than liver. Both sterigmatocystin and zearalenone caused an increase in kidney phagolysosomal fragility at early times after treatment, and all agents except penitrem A caused a decrease in uptake of labeled albumin into kidney cells. Citrinin labelized kidney phagolysosomes in vitro but not in vivo, while only citrinin and zearalenone inhibited in vitro preparations of cathepsin.", "contents": "Effect of mycotoxins on uptake and degradation of [125I] albumin in mouse liver and kidney lysosomes. Some mycotoxins have been evaluated with respect to effects on the reticuloendothelial function of uptake and degradation of a soluble, denatured protein in phagolysosomes isolated from mouse liver and kidneys. Toxins were dissolved in dimethylsulfoxide and administered intraperitoneally. Particulate fractions isolated from liver and kidney homogenates were assayed for proteolytic activity within osmotically active particles (phagolysosomes). Treatment with citrinin, penitrem A, streigmatocystin, and zearalenone produced inhibition of proteolysis in kidney but not in liver phagolysosomes. Moniliformin and T-2 toxin had no detectable effect on lysosome formation or function in either tissue. The phagolysosomal toxicity of these compounds appeared to be more toward kidney than liver. Both sterigmatocystin and zearalenone caused an increase in kidney phagolysosomal fragility at early times after treatment, and all agents except penitrem A caused a decrease in uptake of labeled albumin into kidney cells. Citrinin labelized kidney phagolysosomes in vitro but not in vivo, while only citrinin and zearalenone inhibited in vitro preparations of cathepsin.", "PMID": 966326}
{"id": "pubmed23n0032_6696", "title": "Influence of certain metals on the level of metallothionein-like proteins in the liver and kidneys of rats.", "content": "Rats were given certain metal salts once every other day for six to eight doses and therafter the levels of metallothionein-like proteins (MTP) were determined in their liver and kidneys. The normal level of those proteins ranged from 0.1 to 0.4 mg/g in the liver and 0.2 and 0.6 mg/g in the kidney. Beryllium, magnesium, barium, strontium, tin, arsenic, selenium, chromium, and nickel administration did not influence the tissue levels of MTP. There was a tendency toward increased MTP levels in the liver after the application of high doses of iron. A significant increase in MTP levels in the liver resulted from cobalt administration and in the kidneys of bismuth-treated rats. Applying molecular filtration it was shown that both metals were partially bound in vivo to protein fractions, the molecular weights of which are close to that of metallothionein.", "contents": "Influence of certain metals on the level of metallothionein-like proteins in the liver and kidneys of rats. Rats were given certain metal salts once every other day for six to eight doses and therafter the levels of metallothionein-like proteins (MTP) were determined in their liver and kidneys. The normal level of those proteins ranged from 0.1 to 0.4 mg/g in the liver and 0.2 and 0.6 mg/g in the kidney. Beryllium, magnesium, barium, strontium, tin, arsenic, selenium, chromium, and nickel administration did not influence the tissue levels of MTP. There was a tendency toward increased MTP levels in the liver after the application of high doses of iron. A significant increase in MTP levels in the liver resulted from cobalt administration and in the kidneys of bismuth-treated rats. Applying molecular filtration it was shown that both metals were partially bound in vivo to protein fractions, the molecular weights of which are close to that of metallothionein.", "PMID": 966327}
{"id": "pubmed23n0032_6697", "title": "A quantitative rotational test of vestibular function.", "content": "This paper describes a quantitative vestibular test in which the stimulus is a constant angular acceleration of 3 degrees/sec2 for 80 sec. The nystagmus output is plotted as cumulative slow phase eye displacement against time and is displayed on an oscilloscope screen along with the output from a mathematical model describing vestibulo-oculomotor function. External dials allow one to change the equation constants thereby altering the shape of the model output. Values for equation constants which match the model output to the patient can be read directly from the dials, thus providing a description of the vestibular system under test. Results indicate that vestibular asymmetry (manifest by spontaneous nystagmus is not uncommon in normals, and only when spontaneous nystagmus is coupled with vestibular gain does one get a measure of abnormality. The significance of altered vestibular parameters in certain pathological states is discussed.", "contents": "A quantitative rotational test of vestibular function. This paper describes a quantitative vestibular test in which the stimulus is a constant angular acceleration of 3 degrees/sec2 for 80 sec. The nystagmus output is plotted as cumulative slow phase eye displacement against time and is displayed on an oscilloscope screen along with the output from a mathematical model describing vestibulo-oculomotor function. External dials allow one to change the equation constants thereby altering the shape of the model output. Values for equation constants which match the model output to the patient can be read directly from the dials, thus providing a description of the vestibular system under test. Results indicate that vestibular asymmetry (manifest by spontaneous nystagmus is not uncommon in normals, and only when spontaneous nystagmus is coupled with vestibular gain does one get a measure of abnormality. The significance of altered vestibular parameters in certain pathological states is discussed.", "PMID": 966328}
{"id": "pubmed23n0032_6698", "title": "[Long-term antibiotic therapy in chronic purulent otitis media].", "content": "A new therapeutic approach is proposed for chronic purulent otitis media. This clinical condition is often a recurring phenomenon in spite of all forms of treatment. Ten cases are reviewed and the results encourage the authors to pursue this form of treatment consisting of a longterm antibiotherapy of Ampicilline and Cloxacilline in a further study.", "contents": "[Long-term antibiotic therapy in chronic purulent otitis media]. A new therapeutic approach is proposed for chronic purulent otitis media. This clinical condition is often a recurring phenomenon in spite of all forms of treatment. Ten cases are reviewed and the results encourage the authors to pursue this form of treatment consisting of a longterm antibiotherapy of Ampicilline and Cloxacilline in a further study.", "PMID": 966329}
{"id": "pubmed23n0032_6699", "title": "Hydrocephalus following translabyrinthine surgery.", "content": "Hydrocephalus has been identified and surgically treated with ventricular peritoneal shunts in two of 150 patients following translabyrinthine microsurgical removal of acoustic tumors. This problem has prompted a review of the subject of hydrocephalus in general and \"otitic hydrocephalus\" in particular in relation to contemporary extended temporal bone surgery.", "contents": "Hydrocephalus following translabyrinthine surgery. Hydrocephalus has been identified and surgically treated with ventricular peritoneal shunts in two of 150 patients following translabyrinthine microsurgical removal of acoustic tumors. This problem has prompted a review of the subject of hydrocephalus in general and \"otitic hydrocephalus\" in particular in relation to contemporary extended temporal bone surgery.", "PMID": 966330}
{"id": "pubmed23n0032_6700", "title": "Epidural hemorrhage, a rare complication of otitis media.", "content": "Extradural hemorrhage caused by otitis media is so rare that only four cases were found in a search of the literature. Certain striking similarities and the singular absence of a major demonstrable bleeding vessel in all cases appear more than coincidental and we have proposed a theory to explain the cause of the hemorrhage.", "contents": "Epidural hemorrhage, a rare complication of otitis media. Extradural hemorrhage caused by otitis media is so rare that only four cases were found in a search of the literature. Certain striking similarities and the singular absence of a major demonstrable bleeding vessel in all cases appear more than coincidental and we have proposed a theory to explain the cause of the hemorrhage.", "PMID": 966331}
{"id": "pubmed23n0032_6701", "title": "Bilateral temporal bone metastases of a uterine cervix carcinoma.", "content": "A case is reviewed of bilateral temporal bone metastases from a carcinoma of the uterine cervix. The pertinent literature is reviewed and the temporal bone histology is described.", "contents": "Bilateral temporal bone metastases of a uterine cervix carcinoma. A case is reviewed of bilateral temporal bone metastases from a carcinoma of the uterine cervix. The pertinent literature is reviewed and the temporal bone histology is described.", "PMID": 966332}
{"id": "pubmed23n0032_6702", "title": "Vocal restoration following laryngectomy.", "content": "Methods of vocal restoration after laryngectomy are summarized and a new approach briefly described. It is emphasized that surgical procedures should only be contemplated when a satisfactory esophageal voice has not been achieved.", "contents": "Vocal restoration following laryngectomy. Methods of vocal restoration after laryngectomy are summarized and a new approach briefly described. It is emphasized that surgical procedures should only be contemplated when a satisfactory esophageal voice has not been achieved.", "PMID": 966333}
{"id": "pubmed23n0032_6703", "title": "Systemic lupus erythematosus with a vocal cord granulomatous nodule.", "content": "Systemic lupus erythematosus (SLE) with subcutaneous granulomatous nodules, joint symptoms, and a positive latex titre for rheumatoid factor may cause diagnostic confusion with rheumatoid arthritis (RA). This has been a subject of renewed interest in recent medical literature, but reports of nodules in SLE outside the subcutis are rare. This report presents a patient with SLE and with a vocal cord granulomatous nodule, arthralgia, and a positive latex titre for rheumatoid factor. The laryngeal manifestations of SLE are discussed.", "contents": "Systemic lupus erythematosus with a vocal cord granulomatous nodule. Systemic lupus erythematosus (SLE) with subcutaneous granulomatous nodules, joint symptoms, and a positive latex titre for rheumatoid factor may cause diagnostic confusion with rheumatoid arthritis (RA). This has been a subject of renewed interest in recent medical literature, but reports of nodules in SLE outside the subcutis are rare. This report presents a patient with SLE and with a vocal cord granulomatous nodule, arthralgia, and a positive latex titre for rheumatoid factor. The laryngeal manifestations of SLE are discussed.", "PMID": 966334}
{"id": "pubmed23n0032_6704", "title": "[Non-instrumental traumatic rupture of the hypopharynx and the cervical esophagus].", "content": "We present four cases of non-instrumental traumatic ruptures of the hypopharynx and the esophagus. Three are ruptures with closed neck and one with a rupture associated with a cervical wound. A conjoined lesion of the airway is usual, except in one of our observation. All people injured come from car, sport, or work accidents. The esophageal wound, contaminating the mediastinum, is life-threatening and requires a rapid therapeutic solution: wide drainage tube and antibiotics. The diagnosis, often difficult due to poor symptomatology, must be thought of when there is any severe trauma of the cervical region.", "contents": "[Non-instrumental traumatic rupture of the hypopharynx and the cervical esophagus]. We present four cases of non-instrumental traumatic ruptures of the hypopharynx and the esophagus. Three are ruptures with closed neck and one with a rupture associated with a cervical wound. A conjoined lesion of the airway is usual, except in one of our observation. All people injured come from car, sport, or work accidents. The esophageal wound, contaminating the mediastinum, is life-threatening and requires a rapid therapeutic solution: wide drainage tube and antibiotics. The diagnosis, often difficult due to poor symptomatology, must be thought of when there is any severe trauma of the cervical region.", "PMID": 966335}
{"id": "pubmed23n0032_6705", "title": "Mucocele of the sphenoid sinus presenting as spontaneous pneumocephalus.", "content": "A 60 year old woman presented with headaches and a destructive lesion in the sella turcica. She refused treatment but returned seven years later with a spontaneous pneumocephalus and dementia. A mucocele of the sphenoid sinus was discovered. Her symptoms resolved after removal of the mucocele and obliteration of the sphenoid sinus.", "contents": "Mucocele of the sphenoid sinus presenting as spontaneous pneumocephalus. A 60 year old woman presented with headaches and a destructive lesion in the sella turcica. She refused treatment but returned seven years later with a spontaneous pneumocephalus and dementia. A mucocele of the sphenoid sinus was discovered. Her symptoms resolved after removal of the mucocele and obliteration of the sphenoid sinus.", "PMID": 966336}
{"id": "pubmed23n0032_6706", "title": "[Physiopathology of spontaneous extravasation of urine in the upper urinary tract. Apropos of 24 cases].", "content": "Extravasation of urine was seen in 24 intravenous pyelograms taken for renal colic during the year 1973-74. A number of the patients presented with an acute abdomen. Extravasation took place of retained urine under pressure in the renal pelvis with leakage either around the clayces, from a fissure in the pelvis or even around the ureter. Extravasations of this type are in general rabsorbed without trace. In 5 cases, operation was necessary to drain a purulent collection which had developed in association with the extravasated urine.", "contents": "[Physiopathology of spontaneous extravasation of urine in the upper urinary tract. Apropos of 24 cases]. Extravasation of urine was seen in 24 intravenous pyelograms taken for renal colic during the year 1973-74. A number of the patients presented with an acute abdomen. Extravasation took place of retained urine under pressure in the renal pelvis with leakage either around the clayces, from a fissure in the pelvis or even around the ureter. Extravasations of this type are in general rabsorbed without trace. In 5 cases, operation was necessary to drain a purulent collection which had developed in association with the extravasated urine.", "PMID": 966337}
{"id": "pubmed23n0032_6707", "title": "[Post-operative course in tetraplegics after urological surgers (author's transl)].", "content": "The authors report their experience with 33 tetraplegics undergoing surgery. The operations were varied, endoscopic (resection of the bladder neck, of the prostate or striate sphincter, lithot-itiasis) or surgical (lombotomies, transintentinal cutaneous ureterostomies). The post-operative course may be dramatic or even fatal (acute hypertension with cerebral haemorrhage). Respiratory insufficiency poses no particular problem and can be controlled. Epidural anaesthesia maintained for several days makes it possible to avoid the more serious problems: -- autonomic hyperreflectivity, -- delayed resolution of ileus and digestive complication. The authors hence use epidural anaesthesia routinely and are satisfied with the technique.", "contents": "[Post-operative course in tetraplegics after urological surgers (author's transl)]. The authors report their experience with 33 tetraplegics undergoing surgery. The operations were varied, endoscopic (resection of the bladder neck, of the prostate or striate sphincter, lithot-itiasis) or surgical (lombotomies, transintentinal cutaneous ureterostomies). The post-operative course may be dramatic or even fatal (acute hypertension with cerebral haemorrhage). Respiratory insufficiency poses no particular problem and can be controlled. Epidural anaesthesia maintained for several days makes it possible to avoid the more serious problems: -- autonomic hyperreflectivity, -- delayed resolution of ileus and digestive complication. The authors hence use epidural anaesthesia routinely and are satisfied with the technique.", "PMID": 966338}
{"id": "pubmed23n0032_6708", "title": "[Standardisation of the terminology of function of the lower urinary tract. Incontinence, cystometry, ureteral profile, units of measurement (author's transl)].", "content": "This report contains an initial series of recommendations concerning the terminology of function of the lower urinary tract. It is related specifically to the storage of urine in the bladder, urinary continence and units of measurement. These recommendation were discussed during the 4th Annual Meeting of the International Continence Society at Maintz, Germany in September 1974. This standardisation is proposed in order to facilitate the comparison of results obtained by investigators who use urodynamic methods. It is recommended that the taking into consideration of these standards by noted in written publications, in the \"Material and Methods\" paragraph or its equivalent: \"The methods, definitions and units conforme with the standards proposed by the International Continence Society\".", "contents": "[Standardisation of the terminology of function of the lower urinary tract. Incontinence, cystometry, ureteral profile, units of measurement (author's transl)]. This report contains an initial series of recommendations concerning the terminology of function of the lower urinary tract. It is related specifically to the storage of urine in the bladder, urinary continence and units of measurement. These recommendation were discussed during the 4th Annual Meeting of the International Continence Society at Maintz, Germany in September 1974. This standardisation is proposed in order to facilitate the comparison of results obtained by investigators who use urodynamic methods. It is recommended that the taking into consideration of these standards by noted in written publications, in the \"Material and Methods\" paragraph or its equivalent: \"The methods, definitions and units conforme with the standards proposed by the International Continence Society\".", "PMID": 966339}
{"id": "pubmed23n0032_6709", "title": "[Hypertension due to limited coarctation of the right renal artery occurring 2 years after left nephrectomy for a \"small kidney\". 5 year cure obtained by enlargement angioplasty (author's transl)].", "content": "The case of a young boy developing hypertension 3 years after removal of a small kidney due to pyelonephritis and vesico-ureteral reflux. Discovery in the artery of the remaining kidney of a limited coarctation without any of the lesions usually responsible for hypertension. Cured by enlargement angioplasty with a 5 year follow-up. This case is interpreted as an example (to our knowledge the only case in the literature) of hypertension, the mechanism of which is that revealed by Schlegel's experiments.", "contents": "[Hypertension due to limited coarctation of the right renal artery occurring 2 years after left nephrectomy for a \"small kidney\". 5 year cure obtained by enlargement angioplasty (author's transl)]. The case of a young boy developing hypertension 3 years after removal of a small kidney due to pyelonephritis and vesico-ureteral reflux. Discovery in the artery of the remaining kidney of a limited coarctation without any of the lesions usually responsible for hypertension. Cured by enlargement angioplasty with a 5 year follow-up. This case is interpreted as an example (to our knowledge the only case in the literature) of hypertension, the mechanism of which is that revealed by Schlegel's experiments.", "PMID": 966340}
{"id": "pubmed23n0032_6710", "title": "[Association of a benign renal tumor with renal tuberculosis (author's transl)].", "content": "The authors present what seems to be the first case in the world of the association of urinary tuberculosis with a benign tumor of the kidney.", "contents": "[Association of a benign renal tumor with renal tuberculosis (author's transl)]. The authors present what seems to be the first case in the world of the association of urinary tuberculosis with a benign tumor of the kidney.", "PMID": 966341}
{"id": "pubmed23n0032_6711", "title": "Relationship between intrarenal distribution of blood flow and renin secretion during ureteral occlusion.", "content": "The present study was undertaken to evaluate the relationship between renin secretion from the denervated kidney and intrarenal distribution of blood flow during reductions in renal perfusion pressure by partial constriction of the aorta with and without ureteral occlusion in the anesthetized dog. In addition, renin contents in different zones of the kidney were measured. A reduction in renal arterial pressure from normal pressure (125-135 mmHg) to 77 mm Hg resulted in significant increase in renin secretion and redistribution of cortical blood flow. A further reduction of renal arterial pressure to 51 mmHg produced a marked increase in renin secretion rate (RSR) without further changes in the intrarenal distribution pattern of blood flow. The pressure reductions during ureteral occlusion increased RSR without any change in the distribution pattern of blood flow, and a decrease in the amounts of extractable renin was found in the outer cortex of the experimental kidney. These findings suggest that renin release occurs mainly in the outer cortex, and this process may be stimulated when the mechanism of autoregulation fails as the perfusion presure approaches to the lower range of autoregulation in the outer cortex.", "contents": "Relationship between intrarenal distribution of blood flow and renin secretion during ureteral occlusion. The present study was undertaken to evaluate the relationship between renin secretion from the denervated kidney and intrarenal distribution of blood flow during reductions in renal perfusion pressure by partial constriction of the aorta with and without ureteral occlusion in the anesthetized dog. In addition, renin contents in different zones of the kidney were measured. A reduction in renal arterial pressure from normal pressure (125-135 mmHg) to 77 mm Hg resulted in significant increase in renin secretion and redistribution of cortical blood flow. A further reduction of renal arterial pressure to 51 mmHg produced a marked increase in renin secretion rate (RSR) without further changes in the intrarenal distribution pattern of blood flow. The pressure reductions during ureteral occlusion increased RSR without any change in the distribution pattern of blood flow, and a decrease in the amounts of extractable renin was found in the outer cortex of the experimental kidney. These findings suggest that renin release occurs mainly in the outer cortex, and this process may be stimulated when the mechanism of autoregulation fails as the perfusion presure approaches to the lower range of autoregulation in the outer cortex.", "PMID": 966360}
{"id": "pubmed23n0032_6712", "title": "Epidemiological studies of cerebro-and cardiovascular diseases in the northeast of Japan Part I. Pathological approach to the study in Akita Prefecture.", "content": "To define the etiologic factors of Japanese cerebrovascular diseases, a pathological research was conducted on autopsy cases of stroke in Akita Prefecture. In most cases of cerebral hemorrhages, especially in the middle age group (30-59 of age), arteriosclerotic deviations in basal berebral arteries of the circle of Willis and intracerebral small arteries are not found. The authors conclude that cerebral hemorrhage may occur without relationship to disturbances of lipid metabolism, biochemically, and to atherosclerosis, pathologically. Concerning cerebral infarction, especially in the old generation (over 60 years of age), severe arteriosclerotic deviations were recognized both in basal cerebral and intracerebral arteries. These changes were highly influenced by the grade and duration of hypertension, and rarely influenced by hypercholesterolemia. According to the above-mentioned facts, the results obtained from out epidemiological survey were confirmed by the pathological studies.", "contents": "Epidemiological studies of cerebro-and cardiovascular diseases in the northeast of Japan Part I. Pathological approach to the study in Akita Prefecture. To define the etiologic factors of Japanese cerebrovascular diseases, a pathological research was conducted on autopsy cases of stroke in Akita Prefecture. In most cases of cerebral hemorrhages, especially in the middle age group (30-59 of age), arteriosclerotic deviations in basal berebral arteries of the circle of Willis and intracerebral small arteries are not found. The authors conclude that cerebral hemorrhage may occur without relationship to disturbances of lipid metabolism, biochemically, and to atherosclerosis, pathologically. Concerning cerebral infarction, especially in the old generation (over 60 years of age), severe arteriosclerotic deviations were recognized both in basal cerebral and intracerebral arteries. These changes were highly influenced by the grade and duration of hypertension, and rarely influenced by hypercholesterolemia. According to the above-mentioned facts, the results obtained from out epidemiological survey were confirmed by the pathological studies.", "PMID": 966361}
{"id": "pubmed23n0032_6713", "title": "Quantitative detection of cerebral arteriosclerosis by means of the pressure-velocity hysteresis technique.", "content": "The quantitative detection of cerebral arteriosclerosis by means of the pressure-velocity hysteresis technique composed of the cerebral blood velocity pattern (blood velocity pattern in the internal carotid artery measured by the ultrasonic Doppler technique) and the cerebral pressure pulse pattern (common carotid arteriogram measured by non-invasive sphygmography) was investigated in 17-normal young males, normal middle-aged males and patients with cerebral vascular disorders. The ratio of blood pressure/blood velocity in the maximum blood velocity was used as a measure of resistance of the blood flow. The resistance of the cerebral blood flow was significantlpy increased in the patients with cerebral vascular disorders compared to that in the normal young males. The resistance of cerebral blood flow in the middle-aged males was prone to be intermediate between the former two. The results suggest that the pressure-velocity hysteresis technique is useful for the quantitative detection of cerebral vascular resistance as well as cerebral arteriosclerosis. Several factors related to the technique were discussed.", "contents": "Quantitative detection of cerebral arteriosclerosis by means of the pressure-velocity hysteresis technique. The quantitative detection of cerebral arteriosclerosis by means of the pressure-velocity hysteresis technique composed of the cerebral blood velocity pattern (blood velocity pattern in the internal carotid artery measured by the ultrasonic Doppler technique) and the cerebral pressure pulse pattern (common carotid arteriogram measured by non-invasive sphygmography) was investigated in 17-normal young males, normal middle-aged males and patients with cerebral vascular disorders. The ratio of blood pressure/blood velocity in the maximum blood velocity was used as a measure of resistance of the blood flow. The resistance of the cerebral blood flow was significantlpy increased in the patients with cerebral vascular disorders compared to that in the normal young males. The resistance of cerebral blood flow in the middle-aged males was prone to be intermediate between the former two. The results suggest that the pressure-velocity hysteresis technique is useful for the quantitative detection of cerebral vascular resistance as well as cerebral arteriosclerosis. Several factors related to the technique were discussed.", "PMID": 966362}
{"id": "pubmed23n0032_6714", "title": "Correlation between myocardial and plasma concentration of digitoxin and its metabolites with special reference to digitoxin radio-immunoassay.", "content": "According to our prevous study, the metabolites of digitoxin show nearly dentical affinity with digitoxin antibody. The present study reveals relatively constant ratio of heart to plasma in total 3H-counts, and the concentration of metabolites are ver similar between the two throughout the time investigated which indicates digitoxin concentration of plasma as determined by radioimmunoassay reflects well that of myocardium, although it may contain metabolites.", "contents": "Correlation between myocardial and plasma concentration of digitoxin and its metabolites with special reference to digitoxin radio-immunoassay. According to our prevous study, the metabolites of digitoxin show nearly dentical affinity with digitoxin antibody. The present study reveals relatively constant ratio of heart to plasma in total 3H-counts, and the concentration of metabolites are ver similar between the two throughout the time investigated which indicates digitoxin concentration of plasma as determined by radioimmunoassay reflects well that of myocardium, although it may contain metabolites.", "PMID": 966363}
{"id": "pubmed23n0032_6715", "title": "A histopathological study on the conduction system of the so-called \"Pokkuri disease\" (sudden unexpected cardiac death of unknown origin in Japan).", "content": "The conduction system of seven cases died of the so-called \"Pokkuri disease\", was histopathologically studied with serial sectioning method. Fibrosis with a significant reduction of conduction fibers was observed in the sinoartrial node and junction between the node and atrial muscle fibers in 6 cases. Abnormal course and branching of the sinus node artery was seen in 6 cases of which 3 had no penetration of its main branch into the node. Some pathological lesions exsisted in the atrioventricular conduction system in 4 cases: 2 had fibrotic lesion in the distal bundle of His and proximal left and right bundle branches which were sandwiched between the abnormal conal muscle and the summit of ventricular septum. Remaining 2 had lipomatous partial interruption in the mid-and distal bundle of His. Simultaneous involvement of sinoartrial node and the atrioventricular conduction system was observed in 4 cases. One of such cases showed abnormal ECG consisted of a left axis deviation and right bundle branch block. As a conclusion, pathological lesions in the conduction system are revealed in more than half of cases of \"Pokkuri disease/. The pathgenesis seems to be related to minor anomalies such as abnormal sinus node artery, abnormal conal muscle situation.", "contents": "A histopathological study on the conduction system of the so-called \"Pokkuri disease\" (sudden unexpected cardiac death of unknown origin in Japan). The conduction system of seven cases died of the so-called \"Pokkuri disease\", was histopathologically studied with serial sectioning method. Fibrosis with a significant reduction of conduction fibers was observed in the sinoartrial node and junction between the node and atrial muscle fibers in 6 cases. Abnormal course and branching of the sinus node artery was seen in 6 cases of which 3 had no penetration of its main branch into the node. Some pathological lesions exsisted in the atrioventricular conduction system in 4 cases: 2 had fibrotic lesion in the distal bundle of His and proximal left and right bundle branches which were sandwiched between the abnormal conal muscle and the summit of ventricular septum. Remaining 2 had lipomatous partial interruption in the mid-and distal bundle of His. Simultaneous involvement of sinoartrial node and the atrioventricular conduction system was observed in 4 cases. One of such cases showed abnormal ECG consisted of a left axis deviation and right bundle branch block. As a conclusion, pathological lesions in the conduction system are revealed in more than half of cases of \"Pokkuri disease/. The pathgenesis seems to be related to minor anomalies such as abnormal sinus node artery, abnormal conal muscle situation.", "PMID": 966364}
{"id": "pubmed23n0032_6716", "title": "The concept of afterload mismatch and its implications in the clinical assessment of cardiac contractility.", "content": "The characteristics of left ventricular ejection (velocity and extent of wall shortening) can be analysed in relation to the appropriateness of the matching between afterload and the level of inotropic state (contractility), as modified by the preload (Frank-Starling) reserve. In the normal left ventricle if the preload is not allowed to compensate for an acute increase in afterload, or if the limit of preload reserve is reached, velocity (V CF) and stroke volume will diminish; that is an afterload mismatch occurs. This acute mismatch can be corrected by administration of a positive inotropic agent. In normal conscious animals and in man the ejection phase measures in the basal state  (such as ejection fraction, and VCF corrected for heart size) encompass a relatively narrow range, and when the normal heart adapts successfully to a chronic pressure or volume overload such measures remain normal per unit of muscle. These findings provide the basis for their use in detecting a depressed basal level of inotropic state, even in the presence of certain valvular lesions. When there is mild depression of the basal inotropic state, enhanced preload and dilatation can allow full compensation of VCF, but acute pressure loading can allow detection of the the reduced preload reserve by inducing a substantial fall in stroke volume and VCF. When the basal inotropic state is greatly reduced, a mismatch between afterload and contractility, expressed as reduced VCF or ejection function, will become evident in the basal state even if the afterload is normal. Any increase in aortic pressure will then cause a sharp reduction in stroke volume or VCF. Also, under these circumstances therapeutic afterload reduction with agents such as nitroprusside can increase velocity and extent of wall shortening, and the cardiac output, providing the preload is maintained. The concept of afterload mismatch with limited preload reserve provides a framework for understanding the behavior of the normal or depressed ventrile and how it can operate on a \"descending limb\" of function. It helps to explain why measures of the ejecting phase (which are sensitive to afterload) appear to be more reliable than isovolumic phase indices (which are relatively insensitive to afterload) for detecting depressed basal inotropic state. Finally, the concept allows for interpretation of the responses observed in the clinical setting to acute and chronic increases and decreases in loading conditions on the left ventricle.", "contents": "The concept of afterload mismatch and its implications in the clinical assessment of cardiac contractility. The characteristics of left ventricular ejection (velocity and extent of wall shortening) can be analysed in relation to the appropriateness of the matching between afterload and the level of inotropic state (contractility), as modified by the preload (Frank-Starling) reserve. In the normal left ventricle if the preload is not allowed to compensate for an acute increase in afterload, or if the limit of preload reserve is reached, velocity (V CF) and stroke volume will diminish; that is an afterload mismatch occurs. This acute mismatch can be corrected by administration of a positive inotropic agent. In normal conscious animals and in man the ejection phase measures in the basal state  (such as ejection fraction, and VCF corrected for heart size) encompass a relatively narrow range, and when the normal heart adapts successfully to a chronic pressure or volume overload such measures remain normal per unit of muscle. These findings provide the basis for their use in detecting a depressed basal level of inotropic state, even in the presence of certain valvular lesions. When there is mild depression of the basal inotropic state, enhanced preload and dilatation can allow full compensation of VCF, but acute pressure loading can allow detection of the the reduced preload reserve by inducing a substantial fall in stroke volume and VCF. When the basal inotropic state is greatly reduced, a mismatch between afterload and contractility, expressed as reduced VCF or ejection function, will become evident in the basal state even if the afterload is normal. Any increase in aortic pressure will then cause a sharp reduction in stroke volume or VCF. Also, under these circumstances therapeutic afterload reduction with agents such as nitroprusside can increase velocity and extent of wall shortening, and the cardiac output, providing the preload is maintained. The concept of afterload mismatch with limited preload reserve provides a framework for understanding the behavior of the normal or depressed ventrile and how it can operate on a \"descending limb\" of function. It helps to explain why measures of the ejecting phase (which are sensitive to afterload) appear to be more reliable than isovolumic phase indices (which are relatively insensitive to afterload) for detecting depressed basal inotropic state. Finally, the concept allows for interpretation of the responses observed in the clinical setting to acute and chronic increases and decreases in loading conditions on the left ventricle.", "PMID": 966366}
{"id": "pubmed23n0032_6717", "title": "Vascular protein metabolism in the pathogenesis of hypertension.", "content": "Labelled proline incorporation into collagenous and noncollagenous proteins of aorta or mesenteric arteries was significantly increased in 70-day-old spontaneously hypertensive rats (SHR) at the early stage of hypertension in comparison with normotensive Wistar-Kyoto (WK) rats; however such an increase was not detected in 30-day-old SHR at the prehypertensive stage. Similar increases in the  proline incorporation were noted in 70-day-old renal hypertensive rats and in DOCA hypertensive rats in which hypertension had been induced similarly to that om SHR. Furthermore, the decay of the specific activity of noncollagenous and collagenous proteins was studied for 100 days after labelled proline infusion. The dacay of the noncollagenous protein activity was clearly accelerated in the heart, aorta and especially in the mesenteric arteries of SHR compared with WK. The decrease in the hydroxyproline radioactivity of the collagenous protein was significantly faster in the aorta and mesenteric arteries in SHR. These results proved the increased protein metabolism in the arterial walls in the relatively early stage of hypertension in SHR as well as in experimental hypertension, and then suggested its importance in the common pathogenetic mechanisms of hypertension.", "contents": "Vascular protein metabolism in the pathogenesis of hypertension. Labelled proline incorporation into collagenous and noncollagenous proteins of aorta or mesenteric arteries was significantly increased in 70-day-old spontaneously hypertensive rats (SHR) at the early stage of hypertension in comparison with normotensive Wistar-Kyoto (WK) rats; however such an increase was not detected in 30-day-old SHR at the prehypertensive stage. Similar increases in the  proline incorporation were noted in 70-day-old renal hypertensive rats and in DOCA hypertensive rats in which hypertension had been induced similarly to that om SHR. Furthermore, the decay of the specific activity of noncollagenous and collagenous proteins was studied for 100 days after labelled proline infusion. The dacay of the noncollagenous protein activity was clearly accelerated in the heart, aorta and especially in the mesenteric arteries of SHR compared with WK. The decrease in the hydroxyproline radioactivity of the collagenous protein was significantly faster in the aorta and mesenteric arteries in SHR. These results proved the increased protein metabolism in the arterial walls in the relatively early stage of hypertension in SHR as well as in experimental hypertension, and then suggested its importance in the common pathogenetic mechanisms of hypertension.", "PMID": 966368}
{"id": "pubmed23n0032_6718", "title": "Plasma renin and vascular complications in substrains of the spontaneously hypertensive rat, with a reference to water and electrolyte balance.", "content": "The stroke-prone and stroke-resistant substrains of the spontaneously hypertensive rat (SHR) were employed for an evaluation of possible role of reninangiotensin system and of water and electrolyte balances in inducing hypertensive vascular lesions. Serial study on plasma renin level and vascular changes did not support the hypothesis that plasma renin is a major risk factor in the development of cardiovascular complications. The high plasma renin level seen in advanced stages of hypertension appeared to be a result of severe vascular damages, indicating malignant transformation of hypertension. In this state, water turnover was enhanced without any abnormality in electrolyte balances. A possible mechanism involved in the malignant course of hypertension is discussed.", "contents": "Plasma renin and vascular complications in substrains of the spontaneously hypertensive rat, with a reference to water and electrolyte balance. The stroke-prone and stroke-resistant substrains of the spontaneously hypertensive rat (SHR) were employed for an evaluation of possible role of reninangiotensin system and of water and electrolyte balances in inducing hypertensive vascular lesions. Serial study on plasma renin level and vascular changes did not support the hypothesis that plasma renin is a major risk factor in the development of cardiovascular complications. The high plasma renin level seen in advanced stages of hypertension appeared to be a result of severe vascular damages, indicating malignant transformation of hypertension. In this state, water turnover was enhanced without any abnormality in electrolyte balances. A possible mechanism involved in the malignant course of hypertension is discussed.", "PMID": 966370}
{"id": "pubmed23n0032_6719", "title": "Renin inhibition by synthetic phosphatidyl and phosphorylethanolamines.", "content": "Renin inhibitory effects of about 30 kinds of newly synthesized Phosphatidyl-E and Phosphoryl-E were studied in vitro and in vivo. Among the synthetic Phosphatidyl-E, PE (SEE ARTICLE) SERies were the most potent and these were stronger than natural Phosphatidyl-E. We also confirmed that the converison from the original Phosphatidyl-E, so called prerenininhibitor, to lyso-form to exhibit renin inhibition as mentioned by Sen et al15,16 and Baggio et al.20 was not essential. But a stronger inhibition to renin was observed in PE-72, one of synthetic Phosphoryl-E analogues. PE-104, Phosphoryl-E without long fatty acid chain, was the most potent inhibitor in this study. PE-72 and PE-104 inhibited the reaction between dog renin and substrate in a competitive way. In dogs and rats, Phosphoryl-E decreased hypertensive responce and increase angiotensin I concentration induced by the exogenous renin. Hypotensive effects of Phosphatidyl-E and Phosphoryl-E were also demonstrated in renal hypertensive rats and not in normotensive rats.", "contents": "Renin inhibition by synthetic phosphatidyl and phosphorylethanolamines. Renin inhibitory effects of about 30 kinds of newly synthesized Phosphatidyl-E and Phosphoryl-E were studied in vitro and in vivo. Among the synthetic Phosphatidyl-E, PE (SEE ARTICLE) SERies were the most potent and these were stronger than natural Phosphatidyl-E. We also confirmed that the converison from the original Phosphatidyl-E, so called prerenininhibitor, to lyso-form to exhibit renin inhibition as mentioned by Sen et al15,16 and Baggio et al.20 was not essential. But a stronger inhibition to renin was observed in PE-72, one of synthetic Phosphoryl-E analogues. PE-104, Phosphoryl-E without long fatty acid chain, was the most potent inhibitor in this study. PE-72 and PE-104 inhibited the reaction between dog renin and substrate in a competitive way. In dogs and rats, Phosphoryl-E decreased hypertensive responce and increase angiotensin I concentration induced by the exogenous renin. Hypotensive effects of Phosphatidyl-E and Phosphoryl-E were also demonstrated in renal hypertensive rats and not in normotensive rats.", "PMID": 966372}
{"id": "pubmed23n0032_6720", "title": "Plasma catecholamine levels in the coronary sinus, aorta and femoral vein of subjects undergoing cardiac catheterization at rest and during exercise.", "content": "Plasma catecholamine (CA) levels in the coronary sinus (CS), aorta (Ao) and femoral vein (fv) were simultaneously measured in 22 patients with various heart diseases at rest and during handgrip exercise (IHG). The mean resting levels of plasma norepinephine (NE) in CS, Ao and FV were 359 +/- 49 (SEM) pg/ml, 290 +/- 27 and 234 +/- 24, respectively. The corresponding values of epinephrine (E) were 127 +/- 18 pg/ml, 186+/- 30 and 97 +/- 11, respectively. The E values in Ao were significantly greater than those in CS and in FV (p less than 0.05). IHG exercise induced an obvious elevation of plasma CA levels in every portion of the circulation studied. The mean increments of NE concentration were 81%, 54% and 67% of the resting levels at CS, Ao and FV, respectively, while IHG induced elevation of E were 70% of the resting values at each portion studied. Significant correlations were observed between individual CA concentrations in CS and in Ao, and also between those in Ao and in FV at rest. Under raised sympathoadrenal conditions, however, individual values of NE in CS failed to correlate signficantly to those in Ao and in FV, respectively. The NE output from CS was limited to only 3% and 5% of those in Ao at rest and during IHG, respectively. An actual mean increment of NE on its passing through the coronary circulation was only 2% or less of NE output in ao at both stages. It appears, thus, to be untenable that the cardiac tissue is one of the major source of circulating CA at physiological condition. From these reasons, the direct measurement of NE levels in CS may be mandatory, when plasma CA assay is designed for the purpose of studying the role of the sympathetic nerve activity in the regulation of cardiac function.", "contents": "Plasma catecholamine levels in the coronary sinus, aorta and femoral vein of subjects undergoing cardiac catheterization at rest and during exercise. Plasma catecholamine (CA) levels in the coronary sinus (CS), aorta (Ao) and femoral vein (fv) were simultaneously measured in 22 patients with various heart diseases at rest and during handgrip exercise (IHG). The mean resting levels of plasma norepinephine (NE) in CS, Ao and FV were 359 +/- 49 (SEM) pg/ml, 290 +/- 27 and 234 +/- 24, respectively. The corresponding values of epinephrine (E) were 127 +/- 18 pg/ml, 186+/- 30 and 97 +/- 11, respectively. The E values in Ao were significantly greater than those in CS and in FV (p less than 0.05). IHG exercise induced an obvious elevation of plasma CA levels in every portion of the circulation studied. The mean increments of NE concentration were 81%, 54% and 67% of the resting levels at CS, Ao and FV, respectively, while IHG induced elevation of E were 70% of the resting values at each portion studied. Significant correlations were observed between individual CA concentrations in CS and in Ao, and also between those in Ao and in FV at rest. Under raised sympathoadrenal conditions, however, individual values of NE in CS failed to correlate signficantly to those in Ao and in FV, respectively. The NE output from CS was limited to only 3% and 5% of those in Ao at rest and during IHG, respectively. An actual mean increment of NE on its passing through the coronary circulation was only 2% or less of NE output in ao at both stages. It appears, thus, to be untenable that the cardiac tissue is one of the major source of circulating CA at physiological condition. From these reasons, the direct measurement of NE levels in CS may be mandatory, when plasma CA assay is designed for the purpose of studying the role of the sympathetic nerve activity in the regulation of cardiac function.", "PMID": 966374}
{"id": "pubmed23n0032_6721", "title": "Relationship between renin secretion and renal autoregulation in the dogs.", "content": "The present investigation examined the relationship between renin release and renal autoregulation of blood flow in the anesthetized dogs. A reduction in renal arterial pressure from control pressure to 100mmHg changed neither the flow rates of all cortex zones nor renin secretion. Further reductions of renal arterial pressure to 70mmHg and 50mmHg resulted in a significant increase of renin secretion and a decrease of blood flow in the outer cortex. Intrarenal arterial infusion of acethylcholine abolished an autoregulation of blood flow and pressure reduction to 100mmHg during acetylcholine infusion resulted in a marked increase of renin secretion without any further change in afferent arteriolar resistance. Intrarenal arterial infusion of norepinephrine at a control pressure increased a renin release. However, norepinephrine infusion at reduced pressure suppressed the renin release with recovery a vascular resistance to the control level. These results suggest that the changes in the degree of blood flow and pressure in the renal afferent arterioles are not essential for the renin release, but renin release by the pressure reduction might be related to the autoregulatory capapcity of afferent arterioles in the outer cortex.", "contents": "Relationship between renin secretion and renal autoregulation in the dogs. The present investigation examined the relationship between renin release and renal autoregulation of blood flow in the anesthetized dogs. A reduction in renal arterial pressure from control pressure to 100mmHg changed neither the flow rates of all cortex zones nor renin secretion. Further reductions of renal arterial pressure to 70mmHg and 50mmHg resulted in a significant increase of renin secretion and a decrease of blood flow in the outer cortex. Intrarenal arterial infusion of acethylcholine abolished an autoregulation of blood flow and pressure reduction to 100mmHg during acetylcholine infusion resulted in a marked increase of renin secretion without any further change in afferent arteriolar resistance. Intrarenal arterial infusion of norepinephrine at a control pressure increased a renin release. However, norepinephrine infusion at reduced pressure suppressed the renin release with recovery a vascular resistance to the control level. These results suggest that the changes in the degree of blood flow and pressure in the renal afferent arterioles are not essential for the renin release, but renin release by the pressure reduction might be related to the autoregulatory capapcity of afferent arterioles in the outer cortex.", "PMID": 966375}
{"id": "pubmed23n0032_6722", "title": "An experience in clinical application of real-time two-dimensional echocardiography.", "content": "An experience with a real-time two-dimensional echocardiographic system was presented. In this report a sagittal plane section, i.e., the section which was parallel to the long axis of the left ventricle was used. The two-dimensional section of the normal heart, mitral stenosis, interventricular septal defect (preoperatively and postoperatively), mitral prosthetic valve, pericardial effusion, and acute myocardial infarction were studied. Easy applicability, quick recording, and non-invasive nature of the procedure, make the indications widened including acutely distressed patients. We conclude that real-time two-dimensional echocardiography is one of the most promising cardiac examinations.", "contents": "An experience in clinical application of real-time two-dimensional echocardiography. An experience with a real-time two-dimensional echocardiographic system was presented. In this report a sagittal plane section, i.e., the section which was parallel to the long axis of the left ventricle was used. The two-dimensional section of the normal heart, mitral stenosis, interventricular septal defect (preoperatively and postoperatively), mitral prosthetic valve, pericardial effusion, and acute myocardial infarction were studied. Easy applicability, quick recording, and non-invasive nature of the procedure, make the indications widened including acutely distressed patients. We conclude that real-time two-dimensional echocardiography is one of the most promising cardiac examinations.", "PMID": 966379}
{"id": "pubmed23n0032_6723", "title": "Serum dopamine beta-hydroxylase in cardiovascular diseases.", "content": "Dopamine-beta-hydroxylase (DBH), the enzyme responsible for the biosynthesis of norepinephrine from dopamine was assayed in the blood serum of 23 normal adults, 16 patients with congestive heart failure, 19 patients with asymptomatic ischemic heart disease, 4 patients with angina pectoris at rest, 15 patients with essential hypertension, 8 patients with essential hypotension, 5 patients with neurocirculatory asthenia, and 9 normal adults before and after the exercise test. The serum DBH activity varied within a wide range in the control population. The DBH activity increased about 16% after the exercise stress. A tendency to increase DBH activity was shown in the patients with congestive heart failure. There was no significant difference between asymptomatic ischemic heart disease, essential hypertension and normal controls. A lower value was observed in the patients of angina pectoris at rest, while a higher DBH activity was demonstrated in the patients with essential hypotension. There was no significant difference between the neurocirculatory asthenia group and the normal control group.", "contents": "Serum dopamine beta-hydroxylase in cardiovascular diseases. Dopamine-beta-hydroxylase (DBH), the enzyme responsible for the biosynthesis of norepinephrine from dopamine was assayed in the blood serum of 23 normal adults, 16 patients with congestive heart failure, 19 patients with asymptomatic ischemic heart disease, 4 patients with angina pectoris at rest, 15 patients with essential hypertension, 8 patients with essential hypotension, 5 patients with neurocirculatory asthenia, and 9 normal adults before and after the exercise test. The serum DBH activity varied within a wide range in the control population. The DBH activity increased about 16% after the exercise stress. A tendency to increase DBH activity was shown in the patients with congestive heart failure. There was no significant difference between asymptomatic ischemic heart disease, essential hypertension and normal controls. A lower value was observed in the patients of angina pectoris at rest, while a higher DBH activity was demonstrated in the patients with essential hypotension. There was no significant difference between the neurocirculatory asthenia group and the normal control group.", "PMID": 966380}
{"id": "pubmed23n0032_6724", "title": "Syphilis and its cardiovascular complications in the elderly.", "content": "The prevalence of syphilis and its cardiovascular complications were investigated in the living population more than 60 years of age. The prevalence of positive serologic test for syphilis (STS) were 16.9% (124/736) in males and 17.1% (236/1380) in females, which were not statistically different. Aortic regurgitation (AR) was significantly more frequent in those with positive STS (9.68% in males, 5.08% in females) compared with negative STS (0.98% in males, 1.75% in females) in both sexes (p less than 0.01) and it was marked in males. AR with positive STS had a significantly lower minimal blood pressure than AR with negative STS (p less than 0.05). Calcifications in the ascending aorta were noticed in 5 out of 24 syphilitic AR. Myocardial infarctions were almost equally found among those with positive STS (1.67%) and negative STS (1.65%), and 2 out of 6 syphilitic cases were complicated by AR. The measurement of the aortic width was not valuable for the diagnosis of uncomplicated syphilitic aortitis.", "contents": "Syphilis and its cardiovascular complications in the elderly. The prevalence of syphilis and its cardiovascular complications were investigated in the living population more than 60 years of age. The prevalence of positive serologic test for syphilis (STS) were 16.9% (124/736) in males and 17.1% (236/1380) in females, which were not statistically different. Aortic regurgitation (AR) was significantly more frequent in those with positive STS (9.68% in males, 5.08% in females) compared with negative STS (0.98% in males, 1.75% in females) in both sexes (p less than 0.01) and it was marked in males. AR with positive STS had a significantly lower minimal blood pressure than AR with negative STS (p less than 0.05). Calcifications in the ascending aorta were noticed in 5 out of 24 syphilitic AR. Myocardial infarctions were almost equally found among those with positive STS (1.67%) and negative STS (1.65%), and 2 out of 6 syphilitic cases were complicated by AR. The measurement of the aortic width was not valuable for the diagnosis of uncomplicated syphilitic aortitis.", "PMID": 966381}
{"id": "pubmed23n0032_6725", "title": "Echocardiographic measurement of aortic root diameter.", "content": "One hundred and thirty patients with various heart diseases who underwent angiocardiography were studied to assess the clinical usefulness of echocardiographic aortic root diameter. The echocardiographic aortic root diameter was measured from the anterior surface of the anterior wall to the anterior surface of the posterior wall at end-diastole. A significant correlation was found between aortic root diameters measured by angiocardiograms and those by echocardiograms (r = 0.944, p less than 0.001). The aortic root diameter in normal subjects was 15 to 40 mm with a mean value of 29.0 mm. The corrected aortic root diameter by body surface area in normal subjects ranged from 10.1 to 29.5 mm/M2 with a mean value of 19.18 mm/M2. There appeared to be direct relationship between corrected aortic root diameter and the age. Echocardiography is a simple, safe, and reliable noninvasive method for estimating aortic root diameter.", "contents": "Echocardiographic measurement of aortic root diameter. One hundred and thirty patients with various heart diseases who underwent angiocardiography were studied to assess the clinical usefulness of echocardiographic aortic root diameter. The echocardiographic aortic root diameter was measured from the anterior surface of the anterior wall to the anterior surface of the posterior wall at end-diastole. A significant correlation was found between aortic root diameters measured by angiocardiograms and those by echocardiograms (r = 0.944, p less than 0.001). The aortic root diameter in normal subjects was 15 to 40 mm with a mean value of 29.0 mm. The corrected aortic root diameter by body surface area in normal subjects ranged from 10.1 to 29.5 mm/M2 with a mean value of 19.18 mm/M2. There appeared to be direct relationship between corrected aortic root diameter and the age. Echocardiography is a simple, safe, and reliable noninvasive method for estimating aortic root diameter.", "PMID": 966382}
{"id": "pubmed23n0032_6726", "title": "Severity of coronary sclerosis in the aged: a pathological study in 968 consecutive autopsy cases.", "content": "Severity of the coronary sclerosis was estimated in a total of 968 aged cases of consecutive autopsy. Incidence of myocardial infarction was elevated in the high stenotic indices (sum of the coronary stenosis of the 3 vessels). There were 594 cases (61.4%) of balanced stenosis, 238 cases (24.6%) of unbalanced stenosis, and 136 cases (14.0%) of rare type (extreme difference of degree of stenosis between 3 vessels). There were 155 cases (16.0%) of 3 vessel disease, 154 cases (15.9%) of 2 vessel disease, 215 cases (22.2%) of 1 vessel disease, and 444 cases (45.9%) of no significant coronary sclerosis. Incidence of myocardial infarction in these 4 groups was 47.1%, 22.1%, 9.3%, and 2.3%, respectively. The left anterior descending artery was the leading artery in 1 and 2 vessel diseases, followed by the right coronary artery and then the left circumflex coronary artery.", "contents": "Severity of coronary sclerosis in the aged: a pathological study in 968 consecutive autopsy cases. Severity of the coronary sclerosis was estimated in a total of 968 aged cases of consecutive autopsy. Incidence of myocardial infarction was elevated in the high stenotic indices (sum of the coronary stenosis of the 3 vessels). There were 594 cases (61.4%) of balanced stenosis, 238 cases (24.6%) of unbalanced stenosis, and 136 cases (14.0%) of rare type (extreme difference of degree of stenosis between 3 vessels). There were 155 cases (16.0%) of 3 vessel disease, 154 cases (15.9%) of 2 vessel disease, 215 cases (22.2%) of 1 vessel disease, and 444 cases (45.9%) of no significant coronary sclerosis. Incidence of myocardial infarction in these 4 groups was 47.1%, 22.1%, 9.3%, and 2.3%, respectively. The left anterior descending artery was the leading artery in 1 and 2 vessel diseases, followed by the right coronary artery and then the left circumflex coronary artery.", "PMID": 966383}
{"id": "pubmed23n0032_6727", "title": "Hypotensive action and increased plasma renin activity by Ca2+ antagonist (Nifedipine) in hypertensive patients.", "content": "The Ca2+ antagonistic coronary vasodilator, Nifedipine, was sublingually administered by a dose of 30 mg to 19 patients with hypertension. Blood pressure of patients with with essential hypertension (n=14) decreased from 177 +/- 24 to 123 +/- 13 mmHg systolic and from 108 +/- 12 to 80 +/- 11 mmHg diastolic (mean +/- SD) (p less than 0.01). Plasma renin activity (PRA) increased significantly from 0.73 +/- 0.62 to 1.50 +/- 1.02 ng/ml/h (p less than 0.05). The same tendency was observed in malignant and renovascular hypertension. In primary aldosteronism (n = 2), blood pressure decreased but PRA did not increase. Hypotensive action and increased plasma renin activity by Ca2+ antagonist, Nifedipine, were clearly demonstrated in patients with hypertension.", "contents": "Hypotensive action and increased plasma renin activity by Ca2+ antagonist (Nifedipine) in hypertensive patients. The Ca2+ antagonistic coronary vasodilator, Nifedipine, was sublingually administered by a dose of 30 mg to 19 patients with hypertension. Blood pressure of patients with with essential hypertension (n=14) decreased from 177 +/- 24 to 123 +/- 13 mmHg systolic and from 108 +/- 12 to 80 +/- 11 mmHg diastolic (mean +/- SD) (p less than 0.01). Plasma renin activity (PRA) increased significantly from 0.73 +/- 0.62 to 1.50 +/- 1.02 ng/ml/h (p less than 0.05). The same tendency was observed in malignant and renovascular hypertension. In primary aldosteronism (n = 2), blood pressure decreased but PRA did not increase. Hypotensive action and increased plasma renin activity by Ca2+ antagonist, Nifedipine, were clearly demonstrated in patients with hypertension.", "PMID": 966384}
{"id": "pubmed23n0032_6728", "title": "Influence of alpha- and beta-sympathetic blockade on hypoxic sensitivity of the coronaries.", "content": "The effect of 2 types of adrenergic blockade on the capacity of the coronaries for hypoxic dilatation was studied in open chest dogs under chloralose anesthesia. Reactive hyperemia following the temporary occlusion of the left coronary artery and the vasodilatation elicited by general arterial hypoxia served to determine the range of coronary adaptation. beta-adrenergic blockade induced by propranolol (0.3 mg/Kg i.v. or 0.05 mg/Kg intracoronarially) failed to reduce the range of hypoxic dilatation. On the other hand, the alpha-blocking agent phentolamine (0.45 mg/Kg i.v. or 0.15 mg/Kg i.c.), in association with a decrease of the vascular tone, significantly limited the coronary adaptation to hypoxia.", "contents": "Influence of alpha- and beta-sympathetic blockade on hypoxic sensitivity of the coronaries. The effect of 2 types of adrenergic blockade on the capacity of the coronaries for hypoxic dilatation was studied in open chest dogs under chloralose anesthesia. Reactive hyperemia following the temporary occlusion of the left coronary artery and the vasodilatation elicited by general arterial hypoxia served to determine the range of coronary adaptation. beta-adrenergic blockade induced by propranolol (0.3 mg/Kg i.v. or 0.05 mg/Kg intracoronarially) failed to reduce the range of hypoxic dilatation. On the other hand, the alpha-blocking agent phentolamine (0.45 mg/Kg i.v. or 0.15 mg/Kg i.c.), in association with a decrease of the vascular tone, significantly limited the coronary adaptation to hypoxia.", "PMID": 966385}
{"id": "pubmed23n0032_6729", "title": "Phasic coronary blood flow patterns as related to the balance between oxygen demand and supply in the myocardium.", "content": "To explore possible relationship between changing coronary arterial flow patterns and states of coronary circulation, the left circumflex arterial flow (mean and phasic) was measured simultaneously with the oxygen saturation of the great cardiac vein blood (SGCVO2) in anesthetized open-chest dogs. Coronary vasomotion was altered either with administration of drugs or with mechanical interventions. The \"intermittent\" flow pattern observed in the resting state persisted as far as SGCVO2 remained unchanged or lowered regardless of changes in coronary flow, aortic pressure or heart rate. On the other hand, when increase in circumflex flow occurred simultaneously with elevation of SGCVO2, a \"continuous\" flow pattern of the circumflwx flow was noticed. We suggest that (1) the \"intermittent\" flow pattern would indicate that efficient O2 uptake from the supplied arterial blood is taking place in the myocardium and (2) the \"continuous\" flow pattern would indicate presence of arterial flow excess to the myocardial requirement, rendering the increased coronary blood flow less effective for its amount in terms of O2 supply to the myocardium.", "contents": "Phasic coronary blood flow patterns as related to the balance between oxygen demand and supply in the myocardium. To explore possible relationship between changing coronary arterial flow patterns and states of coronary circulation, the left circumflex arterial flow (mean and phasic) was measured simultaneously with the oxygen saturation of the great cardiac vein blood (SGCVO2) in anesthetized open-chest dogs. Coronary vasomotion was altered either with administration of drugs or with mechanical interventions. The \"intermittent\" flow pattern observed in the resting state persisted as far as SGCVO2 remained unchanged or lowered regardless of changes in coronary flow, aortic pressure or heart rate. On the other hand, when increase in circumflex flow occurred simultaneously with elevation of SGCVO2, a \"continuous\" flow pattern of the circumflwx flow was noticed. We suggest that (1) the \"intermittent\" flow pattern would indicate that efficient O2 uptake from the supplied arterial blood is taking place in the myocardium and (2) the \"continuous\" flow pattern would indicate presence of arterial flow excess to the myocardial requirement, rendering the increased coronary blood flow less effective for its amount in terms of O2 supply to the myocardium.", "PMID": 966386}
{"id": "pubmed23n0032_6730", "title": "Renin and nervous system.", "content": "The renin release is controlled mainly by 3 mechanisms which are mutually not exclusive. The mechanisms can work together or 1 mechanism may play an important role than the other 2. The 3 mechanisms are the sympathetic nervous system, intrarenal baroreceptors, and the macula densa. In this paper, the renin release by electrical stimulation of brain stem, renin release by beta-adrenergic receptor, renin release by catecholamine infusion and angiotensin=sensitive site in brain are reported and discussed.", "contents": "Renin and nervous system. The renin release is controlled mainly by 3 mechanisms which are mutually not exclusive. The mechanisms can work together or 1 mechanism may play an important role than the other 2. The 3 mechanisms are the sympathetic nervous system, intrarenal baroreceptors, and the macula densa. In this paper, the renin release by electrical stimulation of brain stem, renin release by beta-adrenergic receptor, renin release by catecholamine infusion and angiotensin=sensitive site in brain are reported and discussed.", "PMID": 966387}
{"id": "pubmed23n0032_6731", "title": "Positive feedback hypothesis on development of essential hypertension.", "content": "A hypothesis is presented on a possible mechanism of development of the essential hypertension. The present theoretical consideration with Laplace's law and Poiseuille's law indicates that arteriolar constriction increases, unless the blood flow is reduced, both arterial blood pressure and vascular circumferential wall tension which is considered a trigger of medial hypertrophy of the constricted arteriole. If the medial hypertrophy aggravates the vascular constriction, it all the more increases arterial pressure and wall tension. Therefore, the initial arteriolar constriction, however slight, may progressively produce hypertension and augment medial hypertrophy by such a positive feedback mechanism.", "contents": "Positive feedback hypothesis on development of essential hypertension. A hypothesis is presented on a possible mechanism of development of the essential hypertension. The present theoretical consideration with Laplace's law and Poiseuille's law indicates that arteriolar constriction increases, unless the blood flow is reduced, both arterial blood pressure and vascular circumferential wall tension which is considered a trigger of medial hypertrophy of the constricted arteriole. If the medial hypertrophy aggravates the vascular constriction, it all the more increases arterial pressure and wall tension. Therefore, the initial arteriolar constriction, however slight, may progressively produce hypertension and augment medial hypertrophy by such a positive feedback mechanism.", "PMID": 966388}
{"id": "pubmed23n0032_6732", "title": "Changes in P-R interval immediately before, during, and after acute myocardial infarction.", "content": "A 48 years old male developed acute cardiac pain manifested by prolonged P-R interval as the only electrocardiographic sign. Subsequently the electrocardiogram evolved an acute myocardial infarction with a normal P-R interval. Immediately therafter prolonged P-R interval reappeared to become established even after digitalis was long discontinued.", "contents": "Changes in P-R interval immediately before, during, and after acute myocardial infarction. A 48 years old male developed acute cardiac pain manifested by prolonged P-R interval as the only electrocardiographic sign. Subsequently the electrocardiogram evolved an acute myocardial infarction with a normal P-R interval. Immediately therafter prolonged P-R interval reappeared to become established even after digitalis was long discontinued.", "PMID": 966389}
{"id": "pubmed23n0032_6733", "title": "Behaviors of a vigorous protease-producing strain of Staphylocollus aureus in the skin tissue of mice.", "content": "The behaviors of a protease-producing strain of Staphylococcus aureus, including the production of alpha- and beta-hemolysin, protease, and nuclease in the skin tissue of mice, were examined by the fluorescent antibody technique and hematoxylin and eosin staining. When about 10(6) viable cells were subcutaneously inoculated into a mouse, they were phagocytized by leukocytes and a suppurative focus developed in situ. No active extracellular substances other than alpha-hemolysin were demonstrated in this mouse. When 10(7) viable cells were inoculated, most of them were also phagocytized by leukocytes, and a rather large suppurative focus was formed. In this focus a low degree of multiplication of the organisms was observed. Protease and alpha-hemolysin could be detected in the neighborhood of the leukocytes. When 10(8) to 10(9) cells were inoculated, they multiplied transitorily in the subcutaneous tissue and produced the four substances mentioned above. The multiplication of bacterial cells as well as the production of extracellular substances ceased within a comparatively short period and the transitory state was similar to that in a batch fermenter. Necrotic and histolytic lesions were observed only in the mice inoculated with 10(8) to 10(9) viable cells. Similar dermatolytic lesions were also found in mice injected with 0.5 to 1.0 mg of pure protease.", "contents": "Behaviors of a vigorous protease-producing strain of Staphylocollus aureus in the skin tissue of mice. The behaviors of a protease-producing strain of Staphylococcus aureus, including the production of alpha- and beta-hemolysin, protease, and nuclease in the skin tissue of mice, were examined by the fluorescent antibody technique and hematoxylin and eosin staining. When about 10(6) viable cells were subcutaneously inoculated into a mouse, they were phagocytized by leukocytes and a suppurative focus developed in situ. No active extracellular substances other than alpha-hemolysin were demonstrated in this mouse. When 10(7) viable cells were inoculated, most of them were also phagocytized by leukocytes, and a rather large suppurative focus was formed. In this focus a low degree of multiplication of the organisms was observed. Protease and alpha-hemolysin could be detected in the neighborhood of the leukocytes. When 10(8) to 10(9) cells were inoculated, they multiplied transitorily in the subcutaneous tissue and produced the four substances mentioned above. The multiplication of bacterial cells as well as the production of extracellular substances ceased within a comparatively short period and the transitory state was similar to that in a batch fermenter. Necrotic and histolytic lesions were observed only in the mice inoculated with 10(8) to 10(9) viable cells. Similar dermatolytic lesions were also found in mice injected with 0.5 to 1.0 mg of pure protease.", "PMID": 966392}
{"id": "pubmed23n0032_6734", "title": "Studies on delayed hypersensitivity of antigens isolated from Mycoplasma pneumoniae cells.", "content": "Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone-soluble and insoluble fractions. Acetone-insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G-75, yielding three water-soluble fractions which were free from lipid and consisted mainly of polysaccharide-protein complex. All these water-soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement-fixing antibodies. In contrast, the acetone-soluble fraction was reactive with the complement-fixing antibodies but not for the delayed hypersensitivity.", "contents": "Studies on delayed hypersensitivity of antigens isolated from Mycoplasma pneumoniae cells. Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone-soluble and insoluble fractions. Acetone-insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G-75, yielding three water-soluble fractions which were free from lipid and consisted mainly of polysaccharide-protein complex. All these water-soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement-fixing antibodies. In contrast, the acetone-soluble fraction was reactive with the complement-fixing antibodies but not for the delayed hypersensitivity.", "PMID": 966393}
{"id": "pubmed23n0032_6735", "title": "The ability of athymic nude mice to survive intracerebral infection of lymphocytic choriomeningitis (LCM) virus.", "content": "Adult athymic nude mice were protected against the lethality of intracerebral infection with LCM virus. Although the multiplication of LCM virus in the brain was almost equal between nu/nu and nu/+ mice, the delayed hypersensitivity reaction was not evoked in nu/nu mice as evidenced by foot pad reaction.", "contents": "The ability of athymic nude mice to survive intracerebral infection of lymphocytic choriomeningitis (LCM) virus. Adult athymic nude mice were protected against the lethality of intracerebral infection with LCM virus. Although the multiplication of LCM virus in the brain was almost equal between nu/nu and nu/+ mice, the delayed hypersensitivity reaction was not evoked in nu/nu mice as evidenced by foot pad reaction.", "PMID": 966396}
{"id": "pubmed23n0032_6736", "title": "Effects of oxygen supply on electrical and secretory responses of humorally stimulated acinar cells in isolated rat pancreas.", "content": "A technique is described for perfusing isolated rat pancreas with a modified Krebs-Henseleit solution. This preparation made feasible the simultaneous measurement of transmembrane potential, effective membrane resistance, rate of flow of pancreatic juice and amylase output. The hyperpolarizing effect of cholecystokinin-pancreozymin (CCK-PZ) was confirmed by perfusion with a solution containing 5 mU CCK-PZ/ml. The hyperpolarizing efffect of CCK-PZ was enhanced when the pancreas was perfused with an oxygenated solution containing dog erythrocytes. This effect was inhibited by ouabain and anaerobic conditions. It is suggested that the oxidative metabolism supplies energy for a mechanism which is responsible for the hyperpolarizing effect of CCK-PZ. The amylase output and the rate of flow of pancreatic juice were enhanced concurrently under conditions that augumented the hyperpolarizing effect, and they were inhibited under conditions that suppressed the effect. Cellular events in stimulus-secretion coupling in the pancreatic acinar cell are discussed by correlating the electrophysiological responses and the secretory responses under various conditions.", "contents": "Effects of oxygen supply on electrical and secretory responses of humorally stimulated acinar cells in isolated rat pancreas. A technique is described for perfusing isolated rat pancreas with a modified Krebs-Henseleit solution. This preparation made feasible the simultaneous measurement of transmembrane potential, effective membrane resistance, rate of flow of pancreatic juice and amylase output. The hyperpolarizing effect of cholecystokinin-pancreozymin (CCK-PZ) was confirmed by perfusion with a solution containing 5 mU CCK-PZ/ml. The hyperpolarizing efffect of CCK-PZ was enhanced when the pancreas was perfused with an oxygenated solution containing dog erythrocytes. This effect was inhibited by ouabain and anaerobic conditions. It is suggested that the oxidative metabolism supplies energy for a mechanism which is responsible for the hyperpolarizing effect of CCK-PZ. The amylase output and the rate of flow of pancreatic juice were enhanced concurrently under conditions that augumented the hyperpolarizing effect, and they were inhibited under conditions that suppressed the effect. Cellular events in stimulus-secretion coupling in the pancreatic acinar cell are discussed by correlating the electrophysiological responses and the secretory responses under various conditions.", "PMID": 966397}
{"id": "pubmed23n0032_6737", "title": "Role of superficially membrane-bound calcium on excitation-contraction coupling in frog skeletal muscle.", "content": "Single fibers isolated from frog semitendinosus muscle continued to twitch for 5-8 min in calcium-free Ringer solution containing 1 mM ethylene glycol bis (beta-aminoethyl ether)-N, N'-tetraacetic acid (EGTA). Even after twitching was completely abolished, the tension of potassium contracture was not depressed, although the time course was markedly shortened. The resting potential recorded from single fibers 10 min after immersion in EGTA-Ringer solution decreased slightly. These fibers failed to generate action potential, however, in a whole sartorius muscle the self-exchangeable calcium was not significantly reduced by 1mM  EGTA, while it was reduced by about 27% during the potassium contracture induced in the EGTA solution. From these results, it is suggested that the calcium bound on the surface sites of the transverse tubular membrane and the sarcolemma can serve as a trigger for the release of calcium from the sarcoplasmic reticulum and can produce a resting potential and maintain relatively long duration excitability under the condition of extracellular calcium deficiency.", "contents": "Role of superficially membrane-bound calcium on excitation-contraction coupling in frog skeletal muscle. Single fibers isolated from frog semitendinosus muscle continued to twitch for 5-8 min in calcium-free Ringer solution containing 1 mM ethylene glycol bis (beta-aminoethyl ether)-N, N'-tetraacetic acid (EGTA). Even after twitching was completely abolished, the tension of potassium contracture was not depressed, although the time course was markedly shortened. The resting potential recorded from single fibers 10 min after immersion in EGTA-Ringer solution decreased slightly. These fibers failed to generate action potential, however, in a whole sartorius muscle the self-exchangeable calcium was not significantly reduced by 1mM  EGTA, while it was reduced by about 27% during the potassium contracture induced in the EGTA solution. From these results, it is suggested that the calcium bound on the surface sites of the transverse tubular membrane and the sarcolemma can serve as a trigger for the release of calcium from the sarcoplasmic reticulum and can produce a resting potential and maintain relatively long duration excitability under the condition of extracellular calcium deficiency.", "PMID": 966398}
{"id": "pubmed23n0032_6738", "title": "Contracture and hyperpolarization of the rabbit sinoatrial node cells in Na-depleted solution.", "content": "A study was made on both hyperpolarization and contracture which developed in the rabbit sinoatrial node cell during perfusion with Na-depleted solution. The membrane was hyperpolarized in 19 out of 27 specimens (-69.5 +/- 6.0 mV), while in the remaining 8 specimens the value of the membrane potential remained low (-39.4 +/- 2.7 mV). Slope conductance increased as the membrane was hyperpolarized in Na-depleted solution, but not when the value of the membrane potential remained at low. The amplitude of hyperpolarization increased with decrease in Na concentration, increase in Ca concentration and increase in perfusate temperature. A marked increase in diastolic tension was observed in 23 out of 29 specimens but in the 6 remaining specimens the increase in tension was small. Tension increased with decrease in Na concentration, increase in Ca concentration, and increase in perfusate temperature. Pretreatment with ouabain caused a consistent increase in contracture tension, while its effect on the membrane hyperpolarization was variable among different specimens. These results may be explained by assuming that intracellular Ca concentration increased with Na-Ca exchange in Na-depleted solution. In addition to contracture, increase in intracellular Ca concentration might have caused an increase of membrane K conductance and produced marked hyperpolarization.", "contents": "Contracture and hyperpolarization of the rabbit sinoatrial node cells in Na-depleted solution. A study was made on both hyperpolarization and contracture which developed in the rabbit sinoatrial node cell during perfusion with Na-depleted solution. The membrane was hyperpolarized in 19 out of 27 specimens (-69.5 +/- 6.0 mV), while in the remaining 8 specimens the value of the membrane potential remained low (-39.4 +/- 2.7 mV). Slope conductance increased as the membrane was hyperpolarized in Na-depleted solution, but not when the value of the membrane potential remained at low. The amplitude of hyperpolarization increased with decrease in Na concentration, increase in Ca concentration and increase in perfusate temperature. A marked increase in diastolic tension was observed in 23 out of 29 specimens but in the 6 remaining specimens the increase in tension was small. Tension increased with decrease in Na concentration, increase in Ca concentration, and increase in perfusate temperature. Pretreatment with ouabain caused a consistent increase in contracture tension, while its effect on the membrane hyperpolarization was variable among different specimens. These results may be explained by assuming that intracellular Ca concentration increased with Na-Ca exchange in Na-depleted solution. In addition to contracture, increase in intracellular Ca concentration might have caused an increase of membrane K conductance and produced marked hyperpolarization.", "PMID": 966399}
{"id": "pubmed23n0032_6739", "title": "Contraction produced by intracellular injection of calcium, strontium, and barium in the single crayfish muscle fibers.", "content": "Ca ions were ionophoretically injected through an intracellular microelectrode into the single muscle fiber of a crayfish, and the resulting contraction sphere was observed under a microscope and photographed with a movie camera. The minimum contraction produced by the threshold current involved usually three or four, sometimes two, sarcomers on both sides of the injecting pipette but contraction involving only one sarcomere was not observered. The rheobase of the Ca-injecting current was 3.2 X 10(-9) A. The strength-duration curves were determined for Ca-, Sr-, and Ba-injecting currents; all fitted a similar hyperbolic equation. The threshold amount of Ca above rheobasic injection was 2.1 X 10(-15)mol, and the ratios between threshold amounts were Ca: Sr: Ba=1: 1.9: 3.0. The effects of Ca and Sr were additive for the contraction. More current was required for the Ca-injection to produce the contraction in the K-depolarized-or 15mM-procaine-treated muscle, although less current was sufficient for the muscle treated with 0.5-1.0 mM of caffeine. The participation of the Ca-induced Ca release mechanism in the contraction produced by Ca injection and the role of Sr or Ba as a substitute for Ca were discussed.", "contents": "Contraction produced by intracellular injection of calcium, strontium, and barium in the single crayfish muscle fibers. Ca ions were ionophoretically injected through an intracellular microelectrode into the single muscle fiber of a crayfish, and the resulting contraction sphere was observed under a microscope and photographed with a movie camera. The minimum contraction produced by the threshold current involved usually three or four, sometimes two, sarcomers on both sides of the injecting pipette but contraction involving only one sarcomere was not observered. The rheobase of the Ca-injecting current was 3.2 X 10(-9) A. The strength-duration curves were determined for Ca-, Sr-, and Ba-injecting currents; all fitted a similar hyperbolic equation. The threshold amount of Ca above rheobasic injection was 2.1 X 10(-15)mol, and the ratios between threshold amounts were Ca: Sr: Ba=1: 1.9: 3.0. The effects of Ca and Sr were additive for the contraction. More current was required for the Ca-injection to produce the contraction in the K-depolarized-or 15mM-procaine-treated muscle, although less current was sufficient for the muscle treated with 0.5-1.0 mM of caffeine. The participation of the Ca-induced Ca release mechanism in the contraction produced by Ca injection and the role of Sr or Ba as a substitute for Ca were discussed.", "PMID": 966400}
{"id": "pubmed23n0032_6740", "title": "Novel action of a piperazine derivative on the end-plate of the frog.", "content": "The effects of a piperazine derivative, trimetazidine (1-(2, 3, 4-trimethoxybenzyl) piperazine dihydrochloride) on the frog end-plate membrane were studied. Action and resting membrane potentials and the input resistance of muscle fibers were not affected by trimetazidine (82-165 muM). Under these conditions, the frequency of the miniature endplate potentials was unchanged while its amplitude was slightly decreased. The amplitude of acetylcholine (ACh) potentials were markedly and reversibly decreased after application of trimetazine (82-165 muM). The dose response curve of the end-plate membrane to ACh showed a non-competitive type of blockade. Trimetazidine (165 muM) not only decreased the amplitude of the end-plate currents (EPC) recorded from the glycerinated muscles using a voltage clamp technique, but also drastically shortened its time course. Under these conditions, the falling phase of the EPC became completely voltage insensitive. The equilibrium potential for the EPC slightly shifted to a more negative value in the presence of trimetazine (165 muM). Coefficient of variation of EPC was increased by Trimetazidine (165 mum), indicating a decrease in the quantal content of the EPC. The rate of desensitization of the end-plate to ACh was facilitated and the rate of decrease in EPC amplitude during tetanic stimulation became voltage sensitive by the action of trimetazidine (133 muM). It is concluded that trimetazidine mainly acts on the postsynaptic membrane with a weak presynaptic action. The agent seems to block a step subsequent to the interaction of ACh with its receptor, which presumably involves changes in the ion conductance of the membrane and is responsible for the voltage sensitivity of the response.", "contents": "Novel action of a piperazine derivative on the end-plate of the frog. The effects of a piperazine derivative, trimetazidine (1-(2, 3, 4-trimethoxybenzyl) piperazine dihydrochloride) on the frog end-plate membrane were studied. Action and resting membrane potentials and the input resistance of muscle fibers were not affected by trimetazidine (82-165 muM). Under these conditions, the frequency of the miniature endplate potentials was unchanged while its amplitude was slightly decreased. The amplitude of acetylcholine (ACh) potentials were markedly and reversibly decreased after application of trimetazine (82-165 muM). The dose response curve of the end-plate membrane to ACh showed a non-competitive type of blockade. Trimetazidine (165 muM) not only decreased the amplitude of the end-plate currents (EPC) recorded from the glycerinated muscles using a voltage clamp technique, but also drastically shortened its time course. Under these conditions, the falling phase of the EPC became completely voltage insensitive. The equilibrium potential for the EPC slightly shifted to a more negative value in the presence of trimetazine (165 muM). Coefficient of variation of EPC was increased by Trimetazidine (165 mum), indicating a decrease in the quantal content of the EPC. The rate of desensitization of the end-plate to ACh was facilitated and the rate of decrease in EPC amplitude during tetanic stimulation became voltage sensitive by the action of trimetazidine (133 muM). It is concluded that trimetazidine mainly acts on the postsynaptic membrane with a weak presynaptic action. The agent seems to block a step subsequent to the interaction of ACh with its receptor, which presumably involves changes in the ion conductance of the membrane and is responsible for the voltage sensitivity of the response.", "PMID": 966401}
{"id": "pubmed23n0032_6741", "title": "Effects of diets on cold tolerance and metabolic responses to cold in fasted rats.", "content": "Effects of high-fat and high-protein diets on cold tolerance in fasted rats were investigated. High-fat diets caused significant increases in body weight, blood-free fatty acids (FFA), ketone bodies and glucose, while high-protein diet did not modify any of these parameters. Rats on high-fat diets that were exposed to cold after clipping exhibited an intermediate cold tolerance as assessed by the rate of fall in colonic temperature between control rats on a standard diet and cold-acclimated rats. The extent of increase of blood FFA and decrease of blood glucose due to cold exposure was less in the high-fat diet group than in control group, but greater than in cold-acclimated group. The lower fall in colonic temperature due to cold exposure was signifcantly associated with less increase in blood FFA and less decrease in blood glucose. In this relation the high-fat diet group was also intermediate between the control and cold-acclimated groups. The high-protein diet did not make any difference in cold tolerance and cold-induced changes in blood metabolites as compared with those in control standard diet, although it resulted in a marked increase in urinary nitrogen excretion. These results indicate that a high-fat diet could exert a significant favorable effect on cold tolerance in fasted rats, but the effect would not be as much as as in cold acclimated rats.", "contents": "Effects of diets on cold tolerance and metabolic responses to cold in fasted rats. Effects of high-fat and high-protein diets on cold tolerance in fasted rats were investigated. High-fat diets caused significant increases in body weight, blood-free fatty acids (FFA), ketone bodies and glucose, while high-protein diet did not modify any of these parameters. Rats on high-fat diets that were exposed to cold after clipping exhibited an intermediate cold tolerance as assessed by the rate of fall in colonic temperature between control rats on a standard diet and cold-acclimated rats. The extent of increase of blood FFA and decrease of blood glucose due to cold exposure was less in the high-fat diet group than in control group, but greater than in cold-acclimated group. The lower fall in colonic temperature due to cold exposure was signifcantly associated with less increase in blood FFA and less decrease in blood glucose. In this relation the high-fat diet group was also intermediate between the control and cold-acclimated groups. The high-protein diet did not make any difference in cold tolerance and cold-induced changes in blood metabolites as compared with those in control standard diet, although it resulted in a marked increase in urinary nitrogen excretion. These results indicate that a high-fat diet could exert a significant favorable effect on cold tolerance in fasted rats, but the effect would not be as much as as in cold acclimated rats.", "PMID": 966402}
{"id": "pubmed23n0032_6742", "title": "The effects of D600 and verapamil on action potential in the X-organ neuron of the crayfish.", "content": "The effects of D600 and verapamil on the membrane activity of the X-organ neuron of the crayfish were investigated. In TTX solution, D600 and verapamil reversibly reduced the amplitude and the maximum rate of rise of the Ca-dependent action potential as well as delayed rectification. The decrease and the recovery in amplitude of the Ca spike were usually accompanied by parallel changes in delayed rectification. The threshold concentration for observable effects on the Ca spike was from 5 X 10(-6) g/ml to 1X 10(-5) g/ml for both drugs. At 1 X 10(-4) g/ml, the Ca spike was almost blocked. The dose-response relation  with regard to the maximum rate of rise of the Ca spike showed no noticeable difference in effect between D600 and verapamil. By increasing the extracellular Ca concentration, the Ca spike, which had been reduced by these drugs, was restored by the delayed rectification remained to be reduced. In Mn solution, both D600 and verapamil reduced the amplitude and the maximum rate of rise of the Na-dependent action potential as well as the delayed rectification. At 1 X 10(-4) g/ml, the maximum rate of rise of the Na spike was reduced by 30 to 50% with each drug. From the experiments in the Ca and the Mn solutions, it was inferred that the decrease in K activation by these drugs was not brought about secondarily by the decrease in Ca activation but both activation were inhibited independently. It was concluded that both D600 and verapamil are the inhibitors for Ca activation in the X-organ neuron of the crayfish, although they are not Ca specific.", "contents": "The effects of D600 and verapamil on action potential in the X-organ neuron of the crayfish. The effects of D600 and verapamil on the membrane activity of the X-organ neuron of the crayfish were investigated. In TTX solution, D600 and verapamil reversibly reduced the amplitude and the maximum rate of rise of the Ca-dependent action potential as well as delayed rectification. The decrease and the recovery in amplitude of the Ca spike were usually accompanied by parallel changes in delayed rectification. The threshold concentration for observable effects on the Ca spike was from 5 X 10(-6) g/ml to 1X 10(-5) g/ml for both drugs. At 1 X 10(-4) g/ml, the Ca spike was almost blocked. The dose-response relation  with regard to the maximum rate of rise of the Ca spike showed no noticeable difference in effect between D600 and verapamil. By increasing the extracellular Ca concentration, the Ca spike, which had been reduced by these drugs, was restored by the delayed rectification remained to be reduced. In Mn solution, both D600 and verapamil reduced the amplitude and the maximum rate of rise of the Na-dependent action potential as well as the delayed rectification. At 1 X 10(-4) g/ml, the maximum rate of rise of the Na spike was reduced by 30 to 50% with each drug. From the experiments in the Ca and the Mn solutions, it was inferred that the decrease in K activation by these drugs was not brought about secondarily by the decrease in Ca activation but both activation were inhibited independently. It was concluded that both D600 and verapamil are the inhibitors for Ca activation in the X-organ neuron of the crayfish, although they are not Ca specific.", "PMID": 966403}
{"id": "pubmed23n0032_6743", "title": "[Disorders of intraventricular conductivity in ischemic heart disease].", "content": "An analysis of 439 cases of interventricular blocks in ischaemic heart disease is presented, 75.2% of the disorders developing in the branches of the left His' bundle. Intraventricular block was more common in cases of large focal myocardial changes. Bifascicular blocks preceeded full atrioventricular block more often than other forms of intraventricular conductivity dosorders. The greatest difficulties were encountered in the electrocardiographic diagnosis of focal changes in the posterior wall of the left ventricle when the left anterior branch of His' bundle was blocked.", "contents": "[Disorders of intraventricular conductivity in ischemic heart disease]. An analysis of 439 cases of interventricular blocks in ischaemic heart disease is presented, 75.2% of the disorders developing in the branches of the left His' bundle. Intraventricular block was more common in cases of large focal myocardial changes. Bifascicular blocks preceeded full atrioventricular block more often than other forms of intraventricular conductivity dosorders. The greatest difficulties were encountered in the electrocardiographic diagnosis of focal changes in the posterior wall of the left ventricle when the left anterior branch of His' bundle was blocked.", "PMID": 966429}
{"id": "pubmed23n0032_6744", "title": "[Discriminative analysis in detecting cardiac insufficiency in myocardial infarct on the basis of the values of stroke volume and blood lactic acid level].", "content": "In 203 patients with large focal and transmural myocardial infarctions the cardiac output was studied by means of the dye dilution method, as well as the content of the lactic (Barker and Summerson's technique) and pyruvic acids (Travina's technique). Along with the growth of cardiac insufficiency a fall was observed in the cardiac and stroke indices, and a growing content of the lactic and pyruvic acids and of the L/P factor. The stroke index and the blood lactate level proved to be the most informative values. With a stroke index below 24 ml/m2 all patients with myocardial infarction displayed signs of cardiac insufficiency, while with a stroke index above 54.2 ml/m2 cardiac insufficiency was lacking. A stroke index falling between 24 and 54.2 ml/m2 was observed in both the patients with cardiac insufficiency, and in those without it. A lactic acid content above 31.8 mg% was always concomitant with cardiac insufficiency. With the lactic acid level ranging from 16 to 31.8 mg% the probability of cardiac insufficiency was doubtful.", "contents": "[Discriminative analysis in detecting cardiac insufficiency in myocardial infarct on the basis of the values of stroke volume and blood lactic acid level]. In 203 patients with large focal and transmural myocardial infarctions the cardiac output was studied by means of the dye dilution method, as well as the content of the lactic (Barker and Summerson's technique) and pyruvic acids (Travina's technique). Along with the growth of cardiac insufficiency a fall was observed in the cardiac and stroke indices, and a growing content of the lactic and pyruvic acids and of the L/P factor. The stroke index and the blood lactate level proved to be the most informative values. With a stroke index below 24 ml/m2 all patients with myocardial infarction displayed signs of cardiac insufficiency, while with a stroke index above 54.2 ml/m2 cardiac insufficiency was lacking. A stroke index falling between 24 and 54.2 ml/m2 was observed in both the patients with cardiac insufficiency, and in those without it. A lactic acid content above 31.8 mg% was always concomitant with cardiac insufficiency. With the lactic acid level ranging from 16 to 31.8 mg% the probability of cardiac insufficiency was doubtful.", "PMID": 966430}
{"id": "pubmed23n0032_6745", "title": "[Strophanthin sensitivity of animals after coronary artery occlusion and pharmacological action on adrenergic innervation of the heart].", "content": "In 95 feline experiments it was established that coronary artery occlusion does not affect the Strophantin lethal dose, but it increases the sensitivity to the arrhythmogenic effect of the cardiac glycoside after 24-48 hours. Premedication of intact animals with such a beta-adrenergic blocking agent as Anapriline (Inderal) significantly increases the toolerance of Strophantin while the stimulation of the beta-adrenergic structures with Isadrine, on the contrary, decreases the arrhythmogenic and lethal dose of Strophantin. In coronary circulation disorders the pharmacological block of the adrenergic heart innervation did not produce any significant reduction of the sensitivity to the cardiac glycoside.", "contents": "[Strophanthin sensitivity of animals after coronary artery occlusion and pharmacological action on adrenergic innervation of the heart]. In 95 feline experiments it was established that coronary artery occlusion does not affect the Strophantin lethal dose, but it increases the sensitivity to the arrhythmogenic effect of the cardiac glycoside after 24-48 hours. Premedication of intact animals with such a beta-adrenergic blocking agent as Anapriline (Inderal) significantly increases the toolerance of Strophantin while the stimulation of the beta-adrenergic structures with Isadrine, on the contrary, decreases the arrhythmogenic and lethal dose of Strophantin. In coronary circulation disorders the pharmacological block of the adrenergic heart innervation did not produce any significant reduction of the sensitivity to the cardiac glycoside.", "PMID": 966432}
{"id": "pubmed23n0032_6746", "title": "[Study of the relation between cardiac function and the tonus of resistant vessels in experimental disorder of coronary circulation].", "content": "In tests set up on dogs under morphine-chloralose anesthesia features specific for upset cardio- and hemodynamics following a primary and repeated (against the background of a preliminarily provoked myocardial infarction) acute disruption of the coronary circulation were investigated. The development of an acute ischemia of the myocardium in animals with preliminarily induced chronic disruption of the coronary circulation was shown to be attended by more intensive than in healthy dogs hemodynamic shifts and functional disorders of the heart, whereas the reflex component of the reaction, the vasodilating reactions of the peripheral vessels, in particular, proved much less marked. Possible mechanisms accounting for differences in the derangement of the cardio- and hemodynamics in primary and repeated ischemia of the myocardium are discussed.", "contents": "[Study of the relation between cardiac function and the tonus of resistant vessels in experimental disorder of coronary circulation]. In tests set up on dogs under morphine-chloralose anesthesia features specific for upset cardio- and hemodynamics following a primary and repeated (against the background of a preliminarily provoked myocardial infarction) acute disruption of the coronary circulation were investigated. The development of an acute ischemia of the myocardium in animals with preliminarily induced chronic disruption of the coronary circulation was shown to be attended by more intensive than in healthy dogs hemodynamic shifts and functional disorders of the heart, whereas the reflex component of the reaction, the vasodilating reactions of the peripheral vessels, in particular, proved much less marked. Possible mechanisms accounting for differences in the derangement of the cardio- and hemodynamics in primary and repeated ischemia of the myocardium are discussed.", "PMID": 966433}
{"id": "pubmed23n0032_6747", "title": "[Myocardial changes in rats after considerable physical exertion and the effect of pyridoxilate on the severity of these changes].", "content": "Under significant physical exercises the myocardium of rats displays distinct enzymopathy, increased permeability of the cell membranes with a considerable dispersion of plasma proteins and damage to the ultrastructures of the myocytes. The employment of pyridoxilate reduces the severity of the damaging reaction, and to some extent normalizes the structure of the cellular and sub-cellular elements of the myocardium. This indicates to the prospects of using the regulators of cellular respiration under total and tissue hypoxia.", "contents": "[Myocardial changes in rats after considerable physical exertion and the effect of pyridoxilate on the severity of these changes]. Under significant physical exercises the myocardium of rats displays distinct enzymopathy, increased permeability of the cell membranes with a considerable dispersion of plasma proteins and damage to the ultrastructures of the myocytes. The employment of pyridoxilate reduces the severity of the damaging reaction, and to some extent normalizes the structure of the cellular and sub-cellular elements of the myocardium. This indicates to the prospects of using the regulators of cellular respiration under total and tissue hypoxia.", "PMID": 966434}
{"id": "pubmed23n0032_6748", "title": "[Lipid peroxides and arteriosclerosis].", "content": "The significance of free radical oxidation of phospholipids in tissues of animals with experimental atherosclerosis was investigated. By using modern physico-chemical methods an elevated content of polyunsaturated fatty acids and other lipids peroxides was discovered in the blood and the aorta of rabbits with experimental atheromatosis. The human blood demonstrated a low level of protective enzymatic systems and a high content of products secondary to peroxidal oxidation of the lipids. The mechanism accounting for the action of lipids peroxides on the vascular wall resulting in the formation of atheromatous plaques is considered.", "contents": "[Lipid peroxides and arteriosclerosis]. The significance of free radical oxidation of phospholipids in tissues of animals with experimental atherosclerosis was investigated. By using modern physico-chemical methods an elevated content of polyunsaturated fatty acids and other lipids peroxides was discovered in the blood and the aorta of rabbits with experimental atheromatosis. The human blood demonstrated a low level of protective enzymatic systems and a high content of products secondary to peroxidal oxidation of the lipids. The mechanism accounting for the action of lipids peroxides on the vascular wall resulting in the formation of atheromatous plaques is considered.", "PMID": 966438}
{"id": "pubmed23n0032_6749", "title": "[Mechanism of lipoprotein and cholesterol transport into the vascular wall].", "content": "Penetration of radioactive lipoproteins of very low and low density, of free cholesterol and its esters into the aortal wall of rabbits was studied. Close values for ratios between lipids and protein, cholesterol and esterified cholesterol contained in the perfusate's lipoproteins and corresponding lipoprotein fractions separated from normal and atherosclerotic aortas following their perfusion were registered. These findings bear evidence to the fact of atherogenic lipoproteins penetrate the vascular wall in the form of intact particles. Quantitative regularities educed in this connection point to a greater permeability of atherosclerotic aortas to lipoproteins. An analysis of aortal lipids uncombined with lipoproteins showed the ratio between cholesterol and etherified cholesterol to be on the average 5.5 times as high as in the lipoproteins of the aorta and the perfusate. The prevalence of cholesterol in the lipids of the aortal tissue uncombined with lipoproteins points to the existence of other mechanisms governing the entrance of radioactive cholesterol into the vascular wall. Tests in vivo involving intravenous administration of C14-cholesterol yielded the same regularities as \"in vitro\" experiments.", "contents": "[Mechanism of lipoprotein and cholesterol transport into the vascular wall]. Penetration of radioactive lipoproteins of very low and low density, of free cholesterol and its esters into the aortal wall of rabbits was studied. Close values for ratios between lipids and protein, cholesterol and esterified cholesterol contained in the perfusate's lipoproteins and corresponding lipoprotein fractions separated from normal and atherosclerotic aortas following their perfusion were registered. These findings bear evidence to the fact of atherogenic lipoproteins penetrate the vascular wall in the form of intact particles. Quantitative regularities educed in this connection point to a greater permeability of atherosclerotic aortas to lipoproteins. An analysis of aortal lipids uncombined with lipoproteins showed the ratio between cholesterol and etherified cholesterol to be on the average 5.5 times as high as in the lipoproteins of the aorta and the perfusate. The prevalence of cholesterol in the lipids of the aortal tissue uncombined with lipoproteins points to the existence of other mechanisms governing the entrance of radioactive cholesterol into the vascular wall. Tests in vivo involving intravenous administration of C14-cholesterol yielded the same regularities as \"in vitro\" experiments.", "PMID": 966439}
{"id": "pubmed23n0032_6750", "title": "[Role of foam cells in lipoprotein transformation within the vascular wall].", "content": "Foam cells were isolated from atherosclerotic aortas of rabbits following their intima incubation with collagenase and elastase. The viability of the cells, their incidence and nativity were supported histochemically and electron-microscopically. The interaction of the foam cells with lipoproteins was studied in vitro with perfusions of isolated atherosclerotic aortas of rabbits during 6 hours with a medium containing beta- and pre-beta-lipoproteins labelled with I125 or simultaneously with I125 and C14-cholesterol. It was found that after the perfusion the foam cells contain a much higher proportion of radioactivity of the total lipids, cholesterol and cholesterol esters, to that of the proteins, than the perfusate. The foam cells must selectively \"capture\" the lipids from the lipoprotein particles. The proportion \"free cholesterol/cholesterol esters\" (according to the radioactivity) proved to be much higher in the foam cells than in the perfusate. The possible mechanisms of the participation of the foam cells in the metabolism of lipoproteins and in the pathogenesis of atherosclerosis are discussed.", "contents": "[Role of foam cells in lipoprotein transformation within the vascular wall]. Foam cells were isolated from atherosclerotic aortas of rabbits following their intima incubation with collagenase and elastase. The viability of the cells, their incidence and nativity were supported histochemically and electron-microscopically. The interaction of the foam cells with lipoproteins was studied in vitro with perfusions of isolated atherosclerotic aortas of rabbits during 6 hours with a medium containing beta- and pre-beta-lipoproteins labelled with I125 or simultaneously with I125 and C14-cholesterol. It was found that after the perfusion the foam cells contain a much higher proportion of radioactivity of the total lipids, cholesterol and cholesterol esters, to that of the proteins, than the perfusate. The foam cells must selectively \"capture\" the lipids from the lipoprotein particles. The proportion \"free cholesterol/cholesterol esters\" (according to the radioactivity) proved to be much higher in the foam cells than in the perfusate. The possible mechanisms of the participation of the foam cells in the metabolism of lipoproteins and in the pathogenesis of atherosclerosis are discussed.", "PMID": 966440}
{"id": "pubmed23n0032_6751", "title": "[Free hydroxyproline content of the blood serum in patients with coronary arteriosclerosis].", "content": "In 47 patients with atherosclerosis of the coronary arteries the concentration of the blood serum free oxyproline was up by comparison with the normal figure and it depended not so much on the stage of the disease, but rather upon the character of hyperlipoproteinemia. A higher oxyproline concentration was revealed in the blood serum of patients presenting the IIb and IV types of hyperlipoproteinemia. The results of experimental investigations suggest that the activation of the glucocorticoid function of the adrenal cortex in patients with atherosclerosis of the coronary arteries results in a more intensive catabolism of the vascular wall collagen and in attending it rise of the blood serum free oxyproline concentration.", "contents": "[Free hydroxyproline content of the blood serum in patients with coronary arteriosclerosis]. In 47 patients with atherosclerosis of the coronary arteries the concentration of the blood serum free oxyproline was up by comparison with the normal figure and it depended not so much on the stage of the disease, but rather upon the character of hyperlipoproteinemia. A higher oxyproline concentration was revealed in the blood serum of patients presenting the IIb and IV types of hyperlipoproteinemia. The results of experimental investigations suggest that the activation of the glucocorticoid function of the adrenal cortex in patients with atherosclerosis of the coronary arteries results in a more intensive catabolism of the vascular wall collagen and in attending it rise of the blood serum free oxyproline concentration.", "PMID": 966441}
{"id": "pubmed23n0032_6752", "title": "[Various changes in the composition of blood serum phospholipids and lipoproteins in arteriosclerosis].", "content": "The composition of phospholipids of plasma lipoproteins was studied in normal individuals and in patients with coronary atherosclerosis with type IIb and IV hyperlipoproteidemia using two-dimentional thin-layer chromatography. It was demonstrated that in normals the fractions of lipoproteins of a very low, low and high density have an even distribution of most of the phospholipid components, lecithin and sphyngomyelin being the prevailing phospholipids in all fractions of lipoproteins. In type IIb and IV hyperlipoproteidemia, lipoprotein fractions of very low density have a relatively elevated content of lecithin and those of high density have an elevated content of lecithin and lysolecithin and a decreased level of sphyngomyelin. The obtained data are discussed in terms of the possible effect of the chemical composition of phospholipids on the properties of lipoproteins in atherosclerosis.", "contents": "[Various changes in the composition of blood serum phospholipids and lipoproteins in arteriosclerosis]. The composition of phospholipids of plasma lipoproteins was studied in normal individuals and in patients with coronary atherosclerosis with type IIb and IV hyperlipoproteidemia using two-dimentional thin-layer chromatography. It was demonstrated that in normals the fractions of lipoproteins of a very low, low and high density have an even distribution of most of the phospholipid components, lecithin and sphyngomyelin being the prevailing phospholipids in all fractions of lipoproteins. In type IIb and IV hyperlipoproteidemia, lipoprotein fractions of very low density have a relatively elevated content of lecithin and those of high density have an elevated content of lecithin and lysolecithin and a decreased level of sphyngomyelin. The obtained data are discussed in terms of the possible effect of the chemical composition of phospholipids on the properties of lipoproteins in atherosclerosis.", "PMID": 966442}
{"id": "pubmed23n0032_6753", "title": "[Lipid content of the blood serum in relatives of patients with ischemic heart disease].", "content": "The method of calculating the threshold levels of blood plasma cholesterol and triglycerids was analysed. The threshold levels were used for a genetic analysis of the relatives of the probands with coronary heart disease combined with hyperlipoproteinemia. Among 25 relatives of probands suffering coronary heart disease combined with hyperlipoproteinemia the latter was found in 15, while among 25 relatives of probands suffering coronary heart disease and having normal lipid levels hyperlipoproteinemia was found only in 2. The presented data characterise the peculiarities of the diagnosis of familial (primary) hyperlipoproteinemia and support the importance of the hereditary factor in the regulation of lipid metabolsm in the families of coronary atherosclerosis patients.", "contents": "[Lipid content of the blood serum in relatives of patients with ischemic heart disease]. The method of calculating the threshold levels of blood plasma cholesterol and triglycerids was analysed. The threshold levels were used for a genetic analysis of the relatives of the probands with coronary heart disease combined with hyperlipoproteinemia. Among 25 relatives of probands suffering coronary heart disease combined with hyperlipoproteinemia the latter was found in 15, while among 25 relatives of probands suffering coronary heart disease and having normal lipid levels hyperlipoproteinemia was found only in 2. The presented data characterise the peculiarities of the diagnosis of familial (primary) hyperlipoproteinemia and support the importance of the hereditary factor in the regulation of lipid metabolsm in the families of coronary atherosclerosis patients.", "PMID": 966445}
{"id": "pubmed23n0032_6754", "title": "[Plasma renin activity in patients with coronary arteriosclerosis with different types of hyperlipoproteinemia].", "content": "The examination was conducted in 142 patients with coronary atherosclerosis, aged 33 to 74 years, and in 40 normal persons, aged 25 to 48 years. The pain form of the disease was observed in 96 patients, the arrhythmic one--in 38, the painless one--in 8 patients. The arterial pressure was within the normal limits in the examined patients. In 67.6% of the patients hypercholesterolemia was diagnosed, in 47.3%--hypertriglyceridemia. Type II hyperlipoproteinemia was found in 67.6% of the cases, types III and IV--in 5.3 and 9.1%, respectively, type V--in 0.5% of the patients; the type of hyperlipoproteinemia could not be identified in 10.6%, and in 6.9% of the cases the blood level of lipoproteins did not differ from the normal. The plasma renin activity examined by the radioimmunoassay in normals comprised 1.26 +/- 0.21 ng/ml/hour; in patients with the pain form of coronary atherosclerosis--6.67 +/- 0.72 ng/ml/hour; in those with arrhythmias--6.89 +/- 1.20 ng/ml/hour; in those with the painless form--2.39 +/- 1.02 ng/ml/hour. The highest renin activity was revealed in types IIa, IIb and III hyperlipoproteinemia, as well as in paroxysmal arrhythmia and cardiac fibrillation.", "contents": "[Plasma renin activity in patients with coronary arteriosclerosis with different types of hyperlipoproteinemia]. The examination was conducted in 142 patients with coronary atherosclerosis, aged 33 to 74 years, and in 40 normal persons, aged 25 to 48 years. The pain form of the disease was observed in 96 patients, the arrhythmic one--in 38, the painless one--in 8 patients. The arterial pressure was within the normal limits in the examined patients. In 67.6% of the patients hypercholesterolemia was diagnosed, in 47.3%--hypertriglyceridemia. Type II hyperlipoproteinemia was found in 67.6% of the cases, types III and IV--in 5.3 and 9.1%, respectively, type V--in 0.5% of the patients; the type of hyperlipoproteinemia could not be identified in 10.6%, and in 6.9% of the cases the blood level of lipoproteins did not differ from the normal. The plasma renin activity examined by the radioimmunoassay in normals comprised 1.26 +/- 0.21 ng/ml/hour; in patients with the pain form of coronary atherosclerosis--6.67 +/- 0.72 ng/ml/hour; in those with arrhythmias--6.89 +/- 1.20 ng/ml/hour; in those with the painless form--2.39 +/- 1.02 ng/ml/hour. The highest renin activity was revealed in types IIa, IIb and III hyperlipoproteinemia, as well as in paroxysmal arrhythmia and cardiac fibrillation.", "PMID": 966446}
{"id": "pubmed23n0032_6755", "title": "[Quantitative composition of blood plasma prostaglandins in patients with ischemic heart disease].", "content": "From the blood plasma of 34 patients with ischemic heart disease and hyperlipoproteinemia and without it and in 10 practically healthy persons prostaglandins E, A and F2alpha were isolated and the proportion of each one of them was determined by the radioimmunological method. Changes in the quantitative composition of the blood plasma prostaglandins in patients with ischemic heart disease and hyperlipoproteinemia were disclosed, these changes suggesting the participation of the substances in question in the pathogenesis of atherosclerosis.", "contents": "[Quantitative composition of blood plasma prostaglandins in patients with ischemic heart disease]. From the blood plasma of 34 patients with ischemic heart disease and hyperlipoproteinemia and without it and in 10 practically healthy persons prostaglandins E, A and F2alpha were isolated and the proportion of each one of them was determined by the radioimmunological method. Changes in the quantitative composition of the blood plasma prostaglandins in patients with ischemic heart disease and hyperlipoproteinemia were disclosed, these changes suggesting the participation of the substances in question in the pathogenesis of atherosclerosis.", "PMID": 966447}
{"id": "pubmed23n0032_6756", "title": "[Diagnostic possibilities of veloergometry in ischemic heart disease].", "content": "The results of a veloergometric investigation of 160 persons, most of whom were patients with ischemic heart disease, are analyzed. The method of graded physical loads is a necessary complement and continuation of clinical and instrumental examination of patients afflicted with ischemic heart disease. A key electrocardiographic indication of coronary insufficiency identifiable following physical loading is a shift of the ST segment. This sign helps disclose the presence of latent coronary insufficiency in most patients with typical angina pectoris and in 25 per cent of patients with an atypical form of this ailment. In some of the patients with ischemic heart disease the results of the test can be utilized in recognizing early stages of cardiac incompetence. A feature distinguishing the response to a physical load in patients with ischemic heart disease is a greater accretion of systolic pressure than in the case of healthy individuals.", "contents": "[Diagnostic possibilities of veloergometry in ischemic heart disease]. The results of a veloergometric investigation of 160 persons, most of whom were patients with ischemic heart disease, are analyzed. The method of graded physical loads is a necessary complement and continuation of clinical and instrumental examination of patients afflicted with ischemic heart disease. A key electrocardiographic indication of coronary insufficiency identifiable following physical loading is a shift of the ST segment. This sign helps disclose the presence of latent coronary insufficiency in most patients with typical angina pectoris and in 25 per cent of patients with an atypical form of this ailment. In some of the patients with ischemic heart disease the results of the test can be utilized in recognizing early stages of cardiac incompetence. A feature distinguishing the response to a physical load in patients with ischemic heart disease is a greater accretion of systolic pressure than in the case of healthy individuals.", "PMID": 966449}
{"id": "pubmed23n0032_6757", "title": "[Various aspects of the pathogenesis of arteriosclerosis in the light of data of geographic pathology].", "content": "An analysis of 9.550 autopsies of males, aged 10 to 69 years, demonstrated that the time and incidence of the appearance of lipid spots did not depend on the place of residence and ethnic characteristics of the person, while the appearance and further progressive development of fibrous plaques and other more severe kinds of atherosclerotic changes varied greatly from one geographic zone and ethnic group to other. The results of the study of different stages of atherosclerosis and the analysis of literature data support our earlier concept that indicates that fibrous plaques do not necessarily develop from lipid spots, and the latter do not necessarily transform into fibrous plaques.", "contents": "[Various aspects of the pathogenesis of arteriosclerosis in the light of data of geographic pathology]. An analysis of 9.550 autopsies of males, aged 10 to 69 years, demonstrated that the time and incidence of the appearance of lipid spots did not depend on the place of residence and ethnic characteristics of the person, while the appearance and further progressive development of fibrous plaques and other more severe kinds of atherosclerotic changes varied greatly from one geographic zone and ethnic group to other. The results of the study of different stages of atherosclerosis and the analysis of literature data support our earlier concept that indicates that fibrous plaques do not necessarily develop from lipid spots, and the latter do not necessarily transform into fibrous plaques.", "PMID": 966448}
{"id": "pubmed23n0032_6758", "title": "[ECG changes during an attack of stenocardia caused by physical exertion].", "content": "In 85 patients suffering from ischemic heart disease ECG changes appearing at the time of an attack of angina pectoris provoked by a physical effort were studied. An analysis of these changes helps substantiate an objective choice of criteria for stopping a physical load test in this category of patients. As shown by pertinent investigations there is good reason for broadening the criteria for cancelling the load test in persons with a manifest coronary incompetence. A complete scientific justification of criteria for cancelling tests involving physical efforts is possible through contrasting the described changes against coronarographic, clinical and catamnestic findings.", "contents": "[ECG changes during an attack of stenocardia caused by physical exertion]. In 85 patients suffering from ischemic heart disease ECG changes appearing at the time of an attack of angina pectoris provoked by a physical effort were studied. An analysis of these changes helps substantiate an objective choice of criteria for stopping a physical load test in this category of patients. As shown by pertinent investigations there is good reason for broadening the criteria for cancelling the load test in persons with a manifest coronary incompetence. A complete scientific justification of criteria for cancelling tests involving physical efforts is possible through contrasting the described changes against coronarographic, clinical and catamnestic findings.", "PMID": 966450}
{"id": "pubmed23n0032_6759", "title": "Water extraction from the inner medullary collecting tubule system: a role for urea.", "content": "Recent examinations of the inner medullary collecting tubule membrane in vitro have demonstrated that its reflection coefficient to urea (sigma urea) is significantly less than unity and less than sigma NaClhe presence of antidiuretic hormone. Fluid entering the inner medullary collecting tubule has a higher urea concentration and lower NaCl concentration than does the medullary interstitium, although total osmolarity is nearly equal on either side of the membrane. The transtubular difference in solute composition, together with the difference between sigma urea and sigma NaCl, should result in a driving force for extraction of water from the tubule. This hypothesis was examined in a differential analysis of water and solute fluxes across the collecting tubule epitheliu. The results indicate that this driving force contributes significantly to water extraction from the inner medullary collecting tubule.", "contents": "Water extraction from the inner medullary collecting tubule system: a role for urea. Recent examinations of the inner medullary collecting tubule membrane in vitro have demonstrated that its reflection coefficient to urea (sigma urea) is significantly less than unity and less than sigma NaClhe presence of antidiuretic hormone. Fluid entering the inner medullary collecting tubule has a higher urea concentration and lower NaCl concentration than does the medullary interstitium, although total osmolarity is nearly equal on either side of the membrane. The transtubular difference in solute composition, together with the difference between sigma urea and sigma NaCl, should result in a driving force for extraction of water from the tubule. This hypothesis was examined in a differential analysis of water and solute fluxes across the collecting tubule epitheliu. The results indicate that this driving force contributes significantly to water extraction from the inner medullary collecting tubule.", "PMID": 966451}
{"id": "pubmed23n0032_6760", "title": "Mineralocorticoid and glucocorticoid receptors in human kidney.", "content": "Currently, studies on renal mineralocorticoid and glucocorticoid receptors have been confined to experimental animals. In experiments made upon human kidneys removed at operation, tritiated aldosterone has been shown to bind specifically to a single class of cytoplasmic sites which show steroid specificity requisite for physiological mineralocorticoid receptors. Tritiated dexamethasone, similarly, binds specifically to a single class of cytoplasmic sites with affinity characteristics appropriate for glucocorticoid receptors. The similarity between the human renal receptor profile for adrenocorticoids and that found in experimental animals may validate cautions extrapolation from animal models of adrenocorticoid effector mechanisms to man.", "contents": "Mineralocorticoid and glucocorticoid receptors in human kidney. Currently, studies on renal mineralocorticoid and glucocorticoid receptors have been confined to experimental animals. In experiments made upon human kidneys removed at operation, tritiated aldosterone has been shown to bind specifically to a single class of cytoplasmic sites which show steroid specificity requisite for physiological mineralocorticoid receptors. Tritiated dexamethasone, similarly, binds specifically to a single class of cytoplasmic sites with affinity characteristics appropriate for glucocorticoid receptors. The similarity between the human renal receptor profile for adrenocorticoids and that found in experimental animals may validate cautions extrapolation from animal models of adrenocorticoid effector mechanisms to man.", "PMID": 966452}
{"id": "pubmed23n0032_6761", "title": "The role of volume contraction in the hypocalciuric action of chlorothiazide.", "content": "Thiazide-induced hypocalciuria usually requires two to four days of drug treatment for its full expression. The present experiments were designed to test the possibility that an immediate hypocalciuria could be produced in subjects whose extracellular volumes were already contracted. In previously untreated subjects, chlorothiazide (2 g/day) did not produce hypocalciuria on the first day of treatment. Hypocalciuria occurred on the second day and was somewhat enhanced on the third and fourth days of treatment. In subjects pretreated with furosemide (40 mg two or three times a day for two days), chlorothiazide produced hypocalciuria on the first day of its use. Under the influence of chlorothiazide, there was a strong linear correlation between the changes in the excretion of calcium and sodium. The results are consistent with the idea that chlorothiazide enhances the ratio of calcium to sodium reabsorptions and that this tendency for absolute hypocalciuria can be overridden by a large natriuretic response to the drug. Volume contraction, which minimizes the natriretic response to diuretics, allows a more immediate and pronounced hypocalciuric response to thiazides.", "contents": "The role of volume contraction in the hypocalciuric action of chlorothiazide. Thiazide-induced hypocalciuria usually requires two to four days of drug treatment for its full expression. The present experiments were designed to test the possibility that an immediate hypocalciuria could be produced in subjects whose extracellular volumes were already contracted. In previously untreated subjects, chlorothiazide (2 g/day) did not produce hypocalciuria on the first day of treatment. Hypocalciuria occurred on the second day and was somewhat enhanced on the third and fourth days of treatment. In subjects pretreated with furosemide (40 mg two or three times a day for two days), chlorothiazide produced hypocalciuria on the first day of its use. Under the influence of chlorothiazide, there was a strong linear correlation between the changes in the excretion of calcium and sodium. The results are consistent with the idea that chlorothiazide enhances the ratio of calcium to sodium reabsorptions and that this tendency for absolute hypocalciuria can be overridden by a large natriuretic response to the drug. Volume contraction, which minimizes the natriretic response to diuretics, allows a more immediate and pronounced hypocalciuric response to thiazides.", "PMID": 966453}
{"id": "pubmed23n0032_6762", "title": "[Bull's-eye disease (author's transl)].", "content": "Bull's-eye disease is observed in all degenerative maculopathies whether hereditary or acquired, whether primary or secondary, which have as cause an elective or obvious pigment-cell condition. In evolutionary maculardegeneration is bull's-eye disease a temporary occurrence, which precedes the diffuse depigmentation of the macular pigmentepithelium.", "contents": "[Bull's-eye disease (author's transl)]. Bull's-eye disease is observed in all degenerative maculopathies whether hereditary or acquired, whether primary or secondary, which have as cause an elective or obvious pigment-cell condition. In evolutionary maculardegeneration is bull's-eye disease a temporary occurrence, which precedes the diffuse depigmentation of the macular pigmentepithelium.", "PMID": 966559}
{"id": "pubmed23n0032_6763", "title": "[Clinically unsuspected malignant melanomas of the posterior uvea (author's transl)].", "content": "Out of 1300 eyes enucleated between 1966-1974, 300 subsequently revealed histologically-proved malignant melanomas of the uvea. In 264 cases the clinical diagnosis was correctly made. In 36 cases the i.o. malignant melanomas were clinically unsuspected. The incorrect clinical diagnoses included: secondary glaucoma (30), retinal detachment (2), iritis (2), and end/panophthalmitis (2). The clinical symptomatology and morphology of the secondary glaucoma caused by the i.o. melanomas did not differ from that due to other, non-neoplastic etiologies. In almost all of the 36 cases the eyes revealed unilateral severe opacity of the media. It is important that one consider an intraocular melanoma in cases of secondary glaucoma, retinal detachment, or intraocular inflammation in which the etiology is uncertain. This is also true in cases of blind, painful eyes in which there is no obvious etiology. Early diagnosis is of vital interest to the patient because this group, in which the malignant melanoma was unsuspected, may be, as suspected in this study, is characterised by extreme aggressivness and invasive capacity. One third of these eyes revealed extension of tumor through the sclera and optic nerve. Also these tumors often revealed more malignant cell types.", "contents": "[Clinically unsuspected malignant melanomas of the posterior uvea (author's transl)]. Out of 1300 eyes enucleated between 1966-1974, 300 subsequently revealed histologically-proved malignant melanomas of the uvea. In 264 cases the clinical diagnosis was correctly made. In 36 cases the i.o. malignant melanomas were clinically unsuspected. The incorrect clinical diagnoses included: secondary glaucoma (30), retinal detachment (2), iritis (2), and end/panophthalmitis (2). The clinical symptomatology and morphology of the secondary glaucoma caused by the i.o. melanomas did not differ from that due to other, non-neoplastic etiologies. In almost all of the 36 cases the eyes revealed unilateral severe opacity of the media. It is important that one consider an intraocular melanoma in cases of secondary glaucoma, retinal detachment, or intraocular inflammation in which the etiology is uncertain. This is also true in cases of blind, painful eyes in which there is no obvious etiology. Early diagnosis is of vital interest to the patient because this group, in which the malignant melanoma was unsuspected, may be, as suspected in this study, is characterised by extreme aggressivness and invasive capacity. One third of these eyes revealed extension of tumor through the sclera and optic nerve. Also these tumors often revealed more malignant cell types.", "PMID": 966560}
{"id": "pubmed23n0032_6764", "title": "[Tonography after trabeculotomy (author's transl)].", "content": "In 25 eyes suffering from primary open angle glaucoma the facility of outflow was measured before and 1 to 3 months after surgery in consideration of the rigidity. Trabeculotomy improved the facility on an average of 0.14+/-0.02 to 0.28+/-0.03 units. This means that the outflow resistance is decreased from 10.58+/-1.34 to 4.31+/-0.45 units. The statistically highly significant improvement of the outflow conditions must be a consequence of the surgical opening in the trabecular meshwork as the sclera had been closed watertightly at the end of the trabeculotomy. The coefficient of rigidity (graphically determined by applanation tonometry and Schi\u00f6tz tonometry in recumbent position) was decreased from 0.0208+/-0.0010 to 0.0172+/-0.0008. This means that measuring the intraocular pressure postoperatively with a Schi\u00f6tz tonometer results in data which are 3.08+/-0.65 mm Hg lower than the actual pressure level.", "contents": "[Tonography after trabeculotomy (author's transl)]. In 25 eyes suffering from primary open angle glaucoma the facility of outflow was measured before and 1 to 3 months after surgery in consideration of the rigidity. Trabeculotomy improved the facility on an average of 0.14+/-0.02 to 0.28+/-0.03 units. This means that the outflow resistance is decreased from 10.58+/-1.34 to 4.31+/-0.45 units. The statistically highly significant improvement of the outflow conditions must be a consequence of the surgical opening in the trabecular meshwork as the sclera had been closed watertightly at the end of the trabeculotomy. The coefficient of rigidity (graphically determined by applanation tonometry and Schi\u00f6tz tonometry in recumbent position) was decreased from 0.0208+/-0.0010 to 0.0172+/-0.0008. This means that measuring the intraocular pressure postoperatively with a Schi\u00f6tz tonometer results in data which are 3.08+/-0.65 mm Hg lower than the actual pressure level.", "PMID": 966561}
{"id": "pubmed23n0032_6765", "title": "[Hydrophthalmia and configuration of the optic disc (author's transl)].", "content": "Three sisters, one brother, and the father of a female patient with primary hydrophthalmus on both eyes had abnormally great physiologic cups. The cup/disc ratio was between 0.5 and 0.72. Two other brothers showed a cup/disc ratio between 0.37 and 0.42, whereas the mother had a cup/disc ratio of 0.2 on both sides. The enlarged physiologic cups of the sisters, the brother, and the father of the propositus correspond to the 4th category of the classification according to Elschnig. Like the hydrophthalmus as a result of a functional disturbance of the eye cup, the occurrence of abnormally big physiologic cups is also referred to the organizing and forming effects of the eye cup. Whether there exists an intrafamiliar relationship between the hydrophthalmus and the abnormally big physiologic cups or not is to be elucidated by further investigations.", "contents": "[Hydrophthalmia and configuration of the optic disc (author's transl)]. Three sisters, one brother, and the father of a female patient with primary hydrophthalmus on both eyes had abnormally great physiologic cups. The cup/disc ratio was between 0.5 and 0.72. Two other brothers showed a cup/disc ratio between 0.37 and 0.42, whereas the mother had a cup/disc ratio of 0.2 on both sides. The enlarged physiologic cups of the sisters, the brother, and the father of the propositus correspond to the 4th category of the classification according to Elschnig. Like the hydrophthalmus as a result of a functional disturbance of the eye cup, the occurrence of abnormally big physiologic cups is also referred to the organizing and forming effects of the eye cup. Whether there exists an intrafamiliar relationship between the hydrophthalmus and the abnormally big physiologic cups or not is to be elucidated by further investigations.", "PMID": 966562}
{"id": "pubmed23n0032_6766", "title": "[The influence of a metabolic stimulator on the vitality of preserved human corneas (author's transl)].", "content": "20 human donor corneas were placed in a tissue culture medium (TC 199 + 5% Dextran = MK-Medium) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. Another group of 20 human donor eyes were placed in to the same medium with a additional content of 5% Actihaemyl resp. Solcoseryl. Same procedure as above. It could be shown that under the influence of the metabolic activator 22.0% and 23.3% more living endothelial cells could be counted and that the total survival time could be prolonged by 1 1/2 days.", "contents": "[The influence of a metabolic stimulator on the vitality of preserved human corneas (author's transl)]. 20 human donor corneas were placed in a tissue culture medium (TC 199 + 5% Dextran = MK-Medium) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. Another group of 20 human donor eyes were placed in to the same medium with a additional content of 5% Actihaemyl resp. Solcoseryl. Same procedure as above. It could be shown that under the influence of the metabolic activator 22.0% and 23.3% more living endothelial cells could be counted and that the total survival time could be prolonged by 1 1/2 days.", "PMID": 966563}
{"id": "pubmed23n0032_6767", "title": "[Distraneurin (Chlormethiazol) as an anaesthetic for high-risk cases in ophthalmology (author's transl)].", "content": "A new anaesthesia, through Chlormethiazol i.v. is described, which is especially used in cataract (lens) operations. There is no excitation during induction and the depth of anaesthesia (stage) depends on dosage-time ratio. Recovery is calm without undesirable side-effects. The intraocular pressure is significantly diminished during anaesthesia.", "contents": "[Distraneurin (Chlormethiazol) as an anaesthetic for high-risk cases in ophthalmology (author's transl)]. A new anaesthesia, through Chlormethiazol i.v. is described, which is especially used in cataract (lens) operations. There is no excitation during induction and the depth of anaesthesia (stage) depends on dosage-time ratio. Recovery is calm without undesirable side-effects. The intraocular pressure is significantly diminished during anaesthesia.", "PMID": 966564}
{"id": "pubmed23n0032_6768", "title": "[Cataract during intermittent hemodialysis. The influence of hypocalcemia on the development of opacities (author's transl)].", "content": "Twenty uremic patients on intermittent hemodialysis were followed up for lens changes over 4-53 months. Only one patient of 22 years with megaureters and megacystis developed bilateral anterior and posterior subcapsular cataracts during dialysis treatment. The morphology of these cataracts was similar to that of fresh hypocalcemic opacities. The patient had actually been hypocalcemic before the onset of the opacification. Possible reasons for the development of hypocalcemic cataracts during intermittent dialysis might be: 1. a reduced calcium-phosphate ratio due to phosphate accumulation, 2. a decrease of ionized calcium due to removed metabolic acidosis, and 3. a permissive effect of longer latencies.", "contents": "[Cataract during intermittent hemodialysis. The influence of hypocalcemia on the development of opacities (author's transl)]. Twenty uremic patients on intermittent hemodialysis were followed up for lens changes over 4-53 months. Only one patient of 22 years with megaureters and megacystis developed bilateral anterior and posterior subcapsular cataracts during dialysis treatment. The morphology of these cataracts was similar to that of fresh hypocalcemic opacities. The patient had actually been hypocalcemic before the onset of the opacification. Possible reasons for the development of hypocalcemic cataracts during intermittent dialysis might be: 1. a reduced calcium-phosphate ratio due to phosphate accumulation, 2. a decrease of ionized calcium due to removed metabolic acidosis, and 3. a permissive effect of longer latencies.", "PMID": 966565}
{"id": "pubmed23n0032_6769", "title": "[The influence on the speed of epithelialisation of rabbit's eyes of IDU- and tromantadine-containing eye ointments (author's transl)].", "content": "The viro-static drugs IDU and tromantadin were tested in view of their influence on epithelial regeneration. IDU slows down the speed of epithelisation only slightly, and produces no irreversible damage in the corneal stroma, Descemet's membrane and endothelium of the rabbit eye. Tromantadin, however, slows down the speed of epithelisation quite a lot, even if the dosis is relatively small. We also found substantial changes in corneal stroma and Descemet's membrane, which have been partly recognized histologically as fibrous tissue. Application of these medicaments is recommended only if the relation between side effects and viro-static potency has been carefully rated.", "contents": "[The influence on the speed of epithelialisation of rabbit's eyes of IDU- and tromantadine-containing eye ointments (author's transl)]. The viro-static drugs IDU and tromantadin were tested in view of their influence on epithelial regeneration. IDU slows down the speed of epithelisation only slightly, and produces no irreversible damage in the corneal stroma, Descemet's membrane and endothelium of the rabbit eye. Tromantadin, however, slows down the speed of epithelisation quite a lot, even if the dosis is relatively small. We also found substantial changes in corneal stroma and Descemet's membrane, which have been partly recognized histologically as fibrous tissue. Application of these medicaments is recommended only if the relation between side effects and viro-static potency has been carefully rated.", "PMID": 966566}
{"id": "pubmed23n0032_6770", "title": "[Physical symptoms in the orbit during ophthalmodynamography (author's transl)].", "content": "Analysing physical process occurring in the orbit during ophthalmodynamography the author comes to the conclusion that the \"systolic pressure\" as recorded ophthalmodynamographically is not a real pressure present in the ophthalmic artery, but a sum of this pressure and of that which is necessary to overcome the resistence of the orbital tissues. A considerable influence of the blood flow in the system of the external carotid artery is explained by superficial localisation of arterial branches belonging to the system and consequently by direct transmission of volume and pressure changes in the blood flow to the registering device, practically without damping effect of the orbital tissues.", "contents": "[Physical symptoms in the orbit during ophthalmodynamography (author's transl)]. Analysing physical process occurring in the orbit during ophthalmodynamography the author comes to the conclusion that the \"systolic pressure\" as recorded ophthalmodynamographically is not a real pressure present in the ophthalmic artery, but a sum of this pressure and of that which is necessary to overcome the resistence of the orbital tissues. A considerable influence of the blood flow in the system of the external carotid artery is explained by superficial localisation of arterial branches belonging to the system and consequently by direct transmission of volume and pressure changes in the blood flow to the registering device, practically without damping effect of the orbital tissues.", "PMID": 966567}
{"id": "pubmed23n0032_6771", "title": "[The two-way syringe. A comparison to modified aspiration methods in cataract surgery, and more recent indications for its use (author's transl)].", "content": "After description of construction, indication, and application of the two-way syringe in cataract surgery, this instrument is compared to some of its numerous modfications. The original device has the advantage of simplicity, and enables the surgeon to perform simultaneous irrigation and aspiration under zero pressure conditions without an assistant. More recent indications for the use of the two-way syringe are vitreous prolapse, and retinal detachment. These operations are described in detail.", "contents": "[The two-way syringe. A comparison to modified aspiration methods in cataract surgery, and more recent indications for its use (author's transl)]. After description of construction, indication, and application of the two-way syringe in cataract surgery, this instrument is compared to some of its numerous modfications. The original device has the advantage of simplicity, and enables the surgeon to perform simultaneous irrigation and aspiration under zero pressure conditions without an assistant. More recent indications for the use of the two-way syringe are vitreous prolapse, and retinal detachment. These operations are described in detail.", "PMID": 966568}
{"id": "pubmed23n0032_6772", "title": "[Diabetes mellitus--not an aetiological factor of uveitis? (author's transl)].", "content": "Out of 103 patients with uveitis, 14.5% of the cases showed pre-clinical diabetes mellitus. This number exceeds by far the average frequency of approx. 6% of pre-clinical diabetes. On account of these findings, the diabetes mellitus is assumed to be a co-factor of the development of uveitis. In order to clarify the cause of uveitis, the glucose tolerancetest should be given preference in the diagnosis.", "contents": "[Diabetes mellitus--not an aetiological factor of uveitis? (author's transl)]. Out of 103 patients with uveitis, 14.5% of the cases showed pre-clinical diabetes mellitus. This number exceeds by far the average frequency of approx. 6% of pre-clinical diabetes. On account of these findings, the diabetes mellitus is assumed to be a co-factor of the development of uveitis. In order to clarify the cause of uveitis, the glucose tolerancetest should be given preference in the diagnosis.", "PMID": 966569}
{"id": "pubmed23n0032_6773", "title": "[The clinical picture, fluorescence angiography and histologie of a ringmelanoma of the iris (author's transl)].", "content": "Report on a rare case of an iris-ringmelanoma. Arising from the iris base the primary tumor spread in a continuous line along the anterior chamber angle and a shedding of tumor cells in other places of the iris surface could be stated as well. A unilateral dark spotted colouring of the iris with a secondary glaucoma was clinically observed. Gonioscopy, diaphanoscopy, 32P-test, and iris fluorescence-angiography aided the diagnosis. Fluorescence angiography appears to be of particular value in the diagnosis of malignant melanoma of the iris: The peculiar filling pattern seen at the pupillary border of the tumor has up to now only been found in malignant processes of the anterior uvea. The clinical tentative diagnosis of malignant ringmelanoma of the iris, could histopathologically be verified.", "contents": "[The clinical picture, fluorescence angiography and histologie of a ringmelanoma of the iris (author's transl)]. Report on a rare case of an iris-ringmelanoma. Arising from the iris base the primary tumor spread in a continuous line along the anterior chamber angle and a shedding of tumor cells in other places of the iris surface could be stated as well. A unilateral dark spotted colouring of the iris with a secondary glaucoma was clinically observed. Gonioscopy, diaphanoscopy, 32P-test, and iris fluorescence-angiography aided the diagnosis. Fluorescence angiography appears to be of particular value in the diagnosis of malignant melanoma of the iris: The peculiar filling pattern seen at the pupillary border of the tumor has up to now only been found in malignant processes of the anterior uvea. The clinical tentative diagnosis of malignant ringmelanoma of the iris, could histopathologically be verified.", "PMID": 966570}
{"id": "pubmed23n0032_6774", "title": "[A case of healed orbital phlegmona of sinus origin (author's transl)].", "content": "In the etiology of the orbital phlegmona the initial focus originates most frequently in the sinuses. In our case we had the opportunity to observe an orbital phlegmona caused by a purulent inflammation of the sinuses by streptococcus pyogenes (hemolyticus). The course of the process was explosive, with toxic symptoms. After a successful operation and conservative treatment, recovery was achieved.", "contents": "[A case of healed orbital phlegmona of sinus origin (author's transl)]. In the etiology of the orbital phlegmona the initial focus originates most frequently in the sinuses. In our case we had the opportunity to observe an orbital phlegmona caused by a purulent inflammation of the sinuses by streptococcus pyogenes (hemolyticus). The course of the process was explosive, with toxic symptoms. After a successful operation and conservative treatment, recovery was achieved.", "PMID": 966571}
{"id": "pubmed23n0032_6775", "title": "[Fibrosarcoma of the orbit (author's transl)].", "content": "During a period of 8 months a capsulated orbital tumour--not adherent the orbital walls or the bulbus--developed in a 21-year-old female patient. The tumour was exstirpated in toto. The histological findings show a fibrosarcoma with poorly differentiated cells, giant cells and numerous mitoses. The comparatively small incidence of the tumour as well as the prognosis quoad vitam are discussed.", "contents": "[Fibrosarcoma of the orbit (author's transl)]. During a period of 8 months a capsulated orbital tumour--not adherent the orbital walls or the bulbus--developed in a 21-year-old female patient. The tumour was exstirpated in toto. The histological findings show a fibrosarcoma with poorly differentiated cells, giant cells and numerous mitoses. The comparatively small incidence of the tumour as well as the prognosis quoad vitam are discussed.", "PMID": 966572}
{"id": "pubmed23n0032_6776", "title": "[Ocular myasthenia caused by D-penicillamine (author's transl)].", "content": "This report concerns a 55-year-old woman who developed ocular myasthenia after 12 months treatment with D-Penicillamine for her rheumatoid arthritis. In the last 2 years the literature contains reports of 22 similar cases.", "contents": "[Ocular myasthenia caused by D-penicillamine (author's transl)]. This report concerns a 55-year-old woman who developed ocular myasthenia after 12 months treatment with D-Penicillamine for her rheumatoid arthritis. In the last 2 years the literature contains reports of 22 similar cases.", "PMID": 966573}
{"id": "pubmed23n0032_6777", "title": "[Stimulus deprivation amblyopia (author's transl)].", "content": "36 patients who had unilateral occlusion early in life were examined. Only 1 deprivation amblyopia due to unilateral occlusion during 9 weeks was found. Deprivation amblyopias did not occur after unilateral occlusion for periods up to 10 days.", "contents": "[Stimulus deprivation amblyopia (author's transl)]. 36 patients who had unilateral occlusion early in life were examined. Only 1 deprivation amblyopia due to unilateral occlusion during 9 weeks was found. Deprivation amblyopias did not occur after unilateral occlusion for periods up to 10 days.", "PMID": 966574}
{"id": "pubmed23n0032_6778", "title": "[The correction of labile metamorphopsias with cylindrical lenses (author's transl)].", "content": "The author describes a procedure for correction of labile i.e. reversible metamorphopsias by means of cylindrical lenses. The possibility of a curative effect by long-time application of an adequate correction is discussed.", "contents": "[The correction of labile metamorphopsias with cylindrical lenses (author's transl)]. The author describes a procedure for correction of labile i.e. reversible metamorphopsias by means of cylindrical lenses. The possibility of a curative effect by long-time application of an adequate correction is discussed.", "PMID": 966575}
{"id": "pubmed23n0032_6779", "title": "[Observations on long term medication with atropine eye-drops (author's transl)].", "content": "It is reported on two children which had moderate general symptoms after long term application of atropine eye-drops. This was observed the day after discontinuing this medication. The symptoms of nausea, vomiting and perspiration were similar in both children and disappeared after one day. The reason for the symptoms is discussed and it is warned against an excessive application of atropine eye-drops.", "contents": "[Observations on long term medication with atropine eye-drops (author's transl)]. It is reported on two children which had moderate general symptoms after long term application of atropine eye-drops. This was observed the day after discontinuing this medication. The symptoms of nausea, vomiting and perspiration were similar in both children and disappeared after one day. The reason for the symptoms is discussed and it is warned against an excessive application of atropine eye-drops.", "PMID": 966576}
{"id": "pubmed23n0032_6780", "title": "[Spontaneous healing of central-vein-thrombosis in early gravidity (author's transl)].", "content": "The author reports on spontaneous healing of central-vein-thrombosis of both eyes in gravidity (Mens III). The 24-years-old patient is still healthy.", "contents": "[Spontaneous healing of central-vein-thrombosis in early gravidity (author's transl)]. The author reports on spontaneous healing of central-vein-thrombosis of both eyes in gravidity (Mens III). The 24-years-old patient is still healthy.", "PMID": 966577}
{"id": "pubmed23n0032_6781", "title": "[Sclerodermie en coup de sabre with spontaneous filtering bleb (author's transl)].", "content": "In a 43-year-old female patient with sclerodermie en coup de sabre on the right forehead, the following changes of the right eye were observed: Partial lack of hairs of the eyebrow; distortion of the upper eyelid, similar to a coloboma; paralysis of the inferior oblique and the superior rectus muscles; decrease of corneal sensibility; formation of a spontaneous filtering bleb in the upper limbal region; ocular hypotension with changes of the papilla.", "contents": "[Sclerodermie en coup de sabre with spontaneous filtering bleb (author's transl)]. In a 43-year-old female patient with sclerodermie en coup de sabre on the right forehead, the following changes of the right eye were observed: Partial lack of hairs of the eyebrow; distortion of the upper eyelid, similar to a coloboma; paralysis of the inferior oblique and the superior rectus muscles; decrease of corneal sensibility; formation of a spontaneous filtering bleb in the upper limbal region; ocular hypotension with changes of the papilla.", "PMID": 966578}
{"id": "pubmed23n0032_6782", "title": "[Remarks concerning the neuroparalytic keratitis (author's transl)].", "content": "A brief reminder is given of the antagonism between cellular pathology (Virchow) and the pathology of relation (Ricker), as well as a reference to the American paper on the \"Systems of material transport in nerve fibers\" by Sidney Ochs.", "contents": "[Remarks concerning the neuroparalytic keratitis (author's transl)]. A brief reminder is given of the antagonism between cellular pathology (Virchow) and the pathology of relation (Ricker), as well as a reference to the American paper on the \"Systems of material transport in nerve fibers\" by Sidney Ochs.", "PMID": 966579}
{"id": "pubmed23n0032_6783", "title": "[A training device for intraocular surgery (author's transl)].", "content": "Description of a new holder for experimental intraocular surgery on human autopsy eyes. Stability of shape and position of the experimental eyes is achieved by a vacuum surrounding the posterior segment.", "contents": "[A training device for intraocular surgery (author's transl)]. Description of a new holder for experimental intraocular surgery on human autopsy eyes. Stability of shape and position of the experimental eyes is achieved by a vacuum surrounding the posterior segment.", "PMID": 966580}
{"id": "pubmed23n0032_6784", "title": "[Excision of the ciliary-body in cases of secondary closed-angle glaucoma (author's transl)].", "content": "Six cases are reported, in which an excision of the ciliary-body has been performed: 5 cases with secondary closed-angle glaucoma, and one case with secondary open-angle glaucoma. All eyes were aphakic, and in all cases other surgical interventions preceded. The ciliary-body excision is the easiest exactly doseable procedure, compared with other procedures regarding the ciliary-body. Usually excision of the ciliary-body represents the last choice to offer, however in selected cases it might be the method of choice. In this aspect our results are encouraging. The technical details of this procedure are described.", "contents": "[Excision of the ciliary-body in cases of secondary closed-angle glaucoma (author's transl)]. Six cases are reported, in which an excision of the ciliary-body has been performed: 5 cases with secondary closed-angle glaucoma, and one case with secondary open-angle glaucoma. All eyes were aphakic, and in all cases other surgical interventions preceded. The ciliary-body excision is the easiest exactly doseable procedure, compared with other procedures regarding the ciliary-body. Usually excision of the ciliary-body represents the last choice to offer, however in selected cases it might be the method of choice. In this aspect our results are encouraging. The technical details of this procedure are described.", "PMID": 966581}
{"id": "pubmed23n0032_6785", "title": "[Phacolytic and hemolytic glaucoma (author's transl)].", "content": "Clinical and histological data of four patients with acute secondary open-angle glaucoma are described. These included two hemolytic and one phacolytic glaucoma. Another patient showed a transition of hemolytic to a hemosiderotic glaucoma. The hemolytic glaucoma follows a hemorrhage of the vitreous, it is characterised by an open-angle with red-brown pigment deposits on the trabecular meshwork. Macrophages can be isolated from the aqueous humor by zentrifugation. The same macrophages and fragments of lysed red blood cells are histologically visible in the vitreous as well as in the anterior chamber and the trabecular meshwork. The cause of the large vitreous hemorrhage is often a retinal detachment. The hemolytic glaucoma must be clearly distinguished from hemosiderotic and neovascular glaucoma. A transition from hemolytic to hemosiderotic glaucoma is possible. The simultaneous description of hemolytic and phacolytic glaucoma follows by reason of the same pathogenetic mechanism. The treatment of hemolytic glaucoma follows the standard medical therapy of glaucoma. If this therapy will be unsuccessful, a paracentesis with irrigation of the anterior chamber may be required. The only therapy of phacolytic glaucoma is immediate intracapsular cataract extraction.", "contents": "[Phacolytic and hemolytic glaucoma (author's transl)]. Clinical and histological data of four patients with acute secondary open-angle glaucoma are described. These included two hemolytic and one phacolytic glaucoma. Another patient showed a transition of hemolytic to a hemosiderotic glaucoma. The hemolytic glaucoma follows a hemorrhage of the vitreous, it is characterised by an open-angle with red-brown pigment deposits on the trabecular meshwork. Macrophages can be isolated from the aqueous humor by zentrifugation. The same macrophages and fragments of lysed red blood cells are histologically visible in the vitreous as well as in the anterior chamber and the trabecular meshwork. The cause of the large vitreous hemorrhage is often a retinal detachment. The hemolytic glaucoma must be clearly distinguished from hemosiderotic and neovascular glaucoma. A transition from hemolytic to hemosiderotic glaucoma is possible. The simultaneous description of hemolytic and phacolytic glaucoma follows by reason of the same pathogenetic mechanism. The treatment of hemolytic glaucoma follows the standard medical therapy of glaucoma. If this therapy will be unsuccessful, a paracentesis with irrigation of the anterior chamber may be required. The only therapy of phacolytic glaucoma is immediate intracapsular cataract extraction.", "PMID": 966582}
{"id": "pubmed23n0032_6786", "title": "[Echography of the vitreous body in case of aphakia and malignant aphakic glaucoma (author's transl)].", "content": "The senile destructions of the vitreous body which can be seen with the slit-lamp (formation of visible fibres, vacuoles and posterior vitreous detachment) cause, if sufficiently advanced, small echoblips visible at echographic examination. We reexamined aphakic patients who had no complications during cataract cryo-extraction. In aphakic eyes without postoperative vitreous prolapse into the anterior chamber the echograms usually consisted of uninterrupted series of small amplitude echoes in the vitreous area. In aphakic eyes with postoperative vitreous prolapse into the anterior chamber echo-free parts were found in the vitreous area and also parts showing closer placing of vitreous echoes. The echo-free parts may represent fluid-filled vacuoles. In malignant aphakic glaucomas, which could not be controlled otherwise, we succeeded inaspirating aqueous humour from these echo-free parts by punction. Echography facilitated, in this way, the punction of the trapped aqueous. Echography of the senile vitreous destructions approaches the limits of present ultrasonographic facilities; artefacts may occur, and technical improvements are desirable and possible.", "contents": "[Echography of the vitreous body in case of aphakia and malignant aphakic glaucoma (author's transl)]. The senile destructions of the vitreous body which can be seen with the slit-lamp (formation of visible fibres, vacuoles and posterior vitreous detachment) cause, if sufficiently advanced, small echoblips visible at echographic examination. We reexamined aphakic patients who had no complications during cataract cryo-extraction. In aphakic eyes without postoperative vitreous prolapse into the anterior chamber the echograms usually consisted of uninterrupted series of small amplitude echoes in the vitreous area. In aphakic eyes with postoperative vitreous prolapse into the anterior chamber echo-free parts were found in the vitreous area and also parts showing closer placing of vitreous echoes. The echo-free parts may represent fluid-filled vacuoles. In malignant aphakic glaucomas, which could not be controlled otherwise, we succeeded inaspirating aqueous humour from these echo-free parts by punction. Echography facilitated, in this way, the punction of the trapped aqueous. Echography of the senile vitreous destructions approaches the limits of present ultrasonographic facilities; artefacts may occur, and technical improvements are desirable and possible.", "PMID": 966583}
{"id": "pubmed23n0032_6787", "title": "[Computer tomography in ophthalmology (author's transl)].", "content": "The computer-aided tomography system (EMI-Scan) represents a new X-ray method in neuroradiology. Some cases of various space occupying retrobulbar orbit processes are presented, and it is demonstrated that this method is also well suited to the diagnosis of orbit diseases. Physicotechnical basis, technique of examination, the indication for computer tomography and the diagnostic possibilities are described.", "contents": "[Computer tomography in ophthalmology (author's transl)]. The computer-aided tomography system (EMI-Scan) represents a new X-ray method in neuroradiology. Some cases of various space occupying retrobulbar orbit processes are presented, and it is demonstrated that this method is also well suited to the diagnosis of orbit diseases. Physicotechnical basis, technique of examination, the indication for computer tomography and the diagnostic possibilities are described.", "PMID": 966584}
{"id": "pubmed23n0032_6788", "title": "[Computer tomography of the eye and the orbit (author's transl)].", "content": "Princips of computer tomography is demonstrated and the absorption coefficients of ocular and orbital tissue are calculated. The advantages and disadvantages of this procedure are discussed. 39 patients with ocular and orbital diseases were examined by computer tomography. The main indication was tumors, including metastases and diseases arising from ethmoidal cells. Vascular anomalies and tumors were better demonstrated by angiography. Dislocated lens, enlarged tumors of the choroid and prostheses (cerclage and orbital implants) are demonstrable. In contrast to echography, the computer tomography gives certainly better results in the posterior part of the orbit. Technical improvement of the apparatus could lead to a wider use in ophthalmology.", "contents": "[Computer tomography of the eye and the orbit (author's transl)]. Princips of computer tomography is demonstrated and the absorption coefficients of ocular and orbital tissue are calculated. The advantages and disadvantages of this procedure are discussed. 39 patients with ocular and orbital diseases were examined by computer tomography. The main indication was tumors, including metastases and diseases arising from ethmoidal cells. Vascular anomalies and tumors were better demonstrated by angiography. Dislocated lens, enlarged tumors of the choroid and prostheses (cerclage and orbital implants) are demonstrable. In contrast to echography, the computer tomography gives certainly better results in the posterior part of the orbit. Technical improvement of the apparatus could lead to a wider use in ophthalmology.", "PMID": 966585}
{"id": "pubmed23n0032_6789", "title": "[On iris reposition in case of extraction of lens (author's transl)].", "content": "A description is given of a pair of iris retractors which avoid certain shortcomings of other instruments or of other methods of retracting the iris in connection with cryo-extraction, particularly in case where the pupil cannot be dilated or only insufficiently. This pair of iris retractors has proved useful. In addition, information is given about our own technique to achieve a round functional pupil, after complicated lens-extractions with vitreous prolapse, by peripheral iridotomy parallel to the limbus and discission of the body.", "contents": "[On iris reposition in case of extraction of lens (author's transl)]. A description is given of a pair of iris retractors which avoid certain shortcomings of other instruments or of other methods of retracting the iris in connection with cryo-extraction, particularly in case where the pupil cannot be dilated or only insufficiently. This pair of iris retractors has proved useful. In addition, information is given about our own technique to achieve a round functional pupil, after complicated lens-extractions with vitreous prolapse, by peripheral iridotomy parallel to the limbus and discission of the body.", "PMID": 966586}
{"id": "pubmed23n0032_6790", "title": "[Siderosis of the lens (author's transl)].", "content": "Incipient siderosis of the lens is reflected by an extremely fine granular, almost homogenous closely subcapsular brown discoloration between the anterior capsule of the lens and the epithelium. Advanced siderosis of the lens leads to subcapsular \"rust spots\" of varying size, especially in the region below the pupil; to increasing permeability cataract with protein breakdown and also to brown discoloration of the developing hollow spaces and clefts containing protein. In extensive siderosis of the lens, there was the following histological evidence of iron infiltration colour reactions: Between epithelium and capsule: (fusiformly) changed epithelia, intercellular substance formed by metaplasia of epithelia with connective tissue fibrillae (birefractive capsular cataract), in large, balloon-like epithelial cells freely occurring in the liquefied capsule, by decomposition of these cells in all fluid cavities containing protein (water clefts, etc.). On the other hand, there were no siderous granulations of protein: in normal epithelial cells of the lens, in unchanged fibres of the lens, in Wedl's cells and in Morgagni's or myelin droplets.", "contents": "[Siderosis of the lens (author's transl)]. Incipient siderosis of the lens is reflected by an extremely fine granular, almost homogenous closely subcapsular brown discoloration between the anterior capsule of the lens and the epithelium. Advanced siderosis of the lens leads to subcapsular \"rust spots\" of varying size, especially in the region below the pupil; to increasing permeability cataract with protein breakdown and also to brown discoloration of the developing hollow spaces and clefts containing protein. In extensive siderosis of the lens, there was the following histological evidence of iron infiltration colour reactions: Between epithelium and capsule: (fusiformly) changed epithelia, intercellular substance formed by metaplasia of epithelia with connective tissue fibrillae (birefractive capsular cataract), in large, balloon-like epithelial cells freely occurring in the liquefied capsule, by decomposition of these cells in all fluid cavities containing protein (water clefts, etc.). On the other hand, there were no siderous granulations of protein: in normal epithelial cells of the lens, in unchanged fibres of the lens, in Wedl's cells and in Morgagni's or myelin droplets.", "PMID": 966587}
{"id": "pubmed23n0032_6791", "title": "[Corneal pathology in rheumatoid arthritis and Sj\u00f6gren-syndrome with special reference to \"rheumatoid keratomalacia\" (author's transl)].", "content": "The corneal pathology in rheumatoid arthritis and Sj\u00f6gren's syndrome is described with emphasis on rheumatic keratomalacia. This particular disorder whether affecting the central and paracentral or the peripheral parts of the cornea is characterized by thinning of the corneal stroma, loss of epithelium, ulceration and eventually by corneal perforation. 7 cases are reported. Reduced tear production and simultaneous corticosteroid treatment seem to be the essential pathogenetic factors of this corneal disease. Therapeutic consequences and differential diagnosis are discussed.", "contents": "[Corneal pathology in rheumatoid arthritis and Sj\u00f6gren-syndrome with special reference to \"rheumatoid keratomalacia\" (author's transl)]. The corneal pathology in rheumatoid arthritis and Sj\u00f6gren's syndrome is described with emphasis on rheumatic keratomalacia. This particular disorder whether affecting the central and paracentral or the peripheral parts of the cornea is characterized by thinning of the corneal stroma, loss of epithelium, ulceration and eventually by corneal perforation. 7 cases are reported. Reduced tear production and simultaneous corticosteroid treatment seem to be the essential pathogenetic factors of this corneal disease. Therapeutic consequences and differential diagnosis are discussed.", "PMID": 966588}
{"id": "pubmed23n0032_6792", "title": "[Combined contactoptical system (author's transl)].", "content": "The indications and the adaptation method of a combined contactoptical system called piggy-back system, are dealt with. This system comprises the adaptation of a soft contact lens on which is applied afterwards, a hard one. The optical and the therapeutic results obtained in 7 groups of patients (56 eyes) with astigmatism, aphakia, keratokonus, keratoplasty, injuries, chronic lesions of the epithelium, and hyposecretion of the tear fluid, are discussed together with the results of the adaptation of the lenses.", "contents": "[Combined contactoptical system (author's transl)]. The indications and the adaptation method of a combined contactoptical system called piggy-back system, are dealt with. This system comprises the adaptation of a soft contact lens on which is applied afterwards, a hard one. The optical and the therapeutic results obtained in 7 groups of patients (56 eyes) with astigmatism, aphakia, keratokonus, keratoplasty, injuries, chronic lesions of the epithelium, and hyposecretion of the tear fluid, are discussed together with the results of the adaptation of the lenses.", "PMID": 966589}
{"id": "pubmed23n0032_6793", "title": "[Occurrence of diffusions shown by fluorescence angiography of the laser coagulation in animals (author's transl)].", "content": "Laser treated rat fundi were observed angiographically and with the fluorescence microscope for 3 months. Angiographically diffusions have been observable up to about one week post laser, fluorescence microscopically, however, for the whole observation time of 3 months. From the 2nd week, these diffusions were produced mainly by neovascularizations which angiographically were not visible. They started from the choroid and penetrated into the outer part of the retina.", "contents": "[Occurrence of diffusions shown by fluorescence angiography of the laser coagulation in animals (author's transl)]. Laser treated rat fundi were observed angiographically and with the fluorescence microscope for 3 months. Angiographically diffusions have been observable up to about one week post laser, fluorescence microscopically, however, for the whole observation time of 3 months. From the 2nd week, these diffusions were produced mainly by neovascularizations which angiographically were not visible. They started from the choroid and penetrated into the outer part of the retina.", "PMID": 966590}
{"id": "pubmed23n0032_6794", "title": "[The action of pilocarpine on the metabolism of surviving lens epithelium and iris cells (author's transl)].", "content": "In tissue culture, lens epithelia and iris cells survive concentrations of 0.5 mg pilocarpine/100 ml TCM, even better those of 0.25 mg/100 ml. By means of the Warburg-method and Boehringer's Glukotest, the consumption of oxygen and glucose were measured without and with the addition of pilocarpine. The oxygen-uptake of the epithelia decreased to one third after addition of the drug, while it doubled in the iris cells. This reveals a compensating effect of the iris tissue protecting the epithelia. Pilocarpine inhibited the consumption of glucose in both tissues, but not significantly. Consequently, we are not dealing with a cataractogenuous substance. This gives new light upon Hockwin's so-called \"Additions-katarakt\".", "contents": "[The action of pilocarpine on the metabolism of surviving lens epithelium and iris cells (author's transl)]. In tissue culture, lens epithelia and iris cells survive concentrations of 0.5 mg pilocarpine/100 ml TCM, even better those of 0.25 mg/100 ml. By means of the Warburg-method and Boehringer's Glukotest, the consumption of oxygen and glucose were measured without and with the addition of pilocarpine. The oxygen-uptake of the epithelia decreased to one third after addition of the drug, while it doubled in the iris cells. This reveals a compensating effect of the iris tissue protecting the epithelia. Pilocarpine inhibited the consumption of glucose in both tissues, but not significantly. Consequently, we are not dealing with a cataractogenuous substance. This gives new light upon Hockwin's so-called \"Additions-katarakt\".", "PMID": 966591}
{"id": "pubmed23n0032_6795", "title": "[The clinical picture of retinopathia oxalogenica (author's transl)].", "content": "A presentation is given of the severe changes in the retina of a 26-year-old man caused by intraocular Calciumoxalate crystals with clinical, histological and polarizing optical findings. The stadia of the retinopathia oxalogenica are classified here.", "contents": "[The clinical picture of retinopathia oxalogenica (author's transl)]. A presentation is given of the severe changes in the retina of a 26-year-old man caused by intraocular Calciumoxalate crystals with clinical, histological and polarizing optical findings. The stadia of the retinopathia oxalogenica are classified here.", "PMID": 966592}
{"id": "pubmed23n0032_6796", "title": "[Alport-syndrom with participation of the cornea and alterations of ERG (author's transl)].", "content": "A description of a 26-year-old patient who had hearing difficulties, progressive nephropathy, and conus of the lens in both eyes is given. The ERG was below normal and the EOG remained normal. About the same time the nephropathy affliction occurred, a ringed abscess appeared in the left eye. Afterwards it healed with a scar, but half a year later the same affliction appeared in the right eye.", "contents": "[Alport-syndrom with participation of the cornea and alterations of ERG (author's transl)]. A description of a 26-year-old patient who had hearing difficulties, progressive nephropathy, and conus of the lens in both eyes is given. The ERG was below normal and the EOG remained normal. About the same time the nephropathy affliction occurred, a ringed abscess appeared in the left eye. Afterwards it healed with a scar, but half a year later the same affliction appeared in the right eye.", "PMID": 966593}
{"id": "pubmed23n0032_6797", "title": "[Tuberous sclerosis with megalocornea and coloboma of the iris (author's transl)].", "content": "A mentally and physically retarded 4 1/2 year-old boy with epileptic seizures showed a megalocornea on both sides, a coloboma of the iris in the right eye and a white area at the temporal side of the disc in the left eye. At first a coloboma of the disc was suspected. By further controls at the age of 8 years a typical two diopters elevated nodular opaque white mass was seen in place of the white area in the left eye, in addition two flat tumours were also seen. In the right eye with coloboma of the iris there was also a flat area. Radiographically the right kidney showed two ureters with flat calyces. At the age of 8 years symmetrical face naevi occurred only under atropine medication, and showed at the age of 10 years the typical picture of Pringle's tumours.", "contents": "[Tuberous sclerosis with megalocornea and coloboma of the iris (author's transl)]. A mentally and physically retarded 4 1/2 year-old boy with epileptic seizures showed a megalocornea on both sides, a coloboma of the iris in the right eye and a white area at the temporal side of the disc in the left eye. At first a coloboma of the disc was suspected. By further controls at the age of 8 years a typical two diopters elevated nodular opaque white mass was seen in place of the white area in the left eye, in addition two flat tumours were also seen. In the right eye with coloboma of the iris there was also a flat area. Radiographically the right kidney showed two ureters with flat calyces. At the age of 8 years symmetrical face naevi occurred only under atropine medication, and showed at the age of 10 years the typical picture of Pringle's tumours.", "PMID": 966594}
{"id": "pubmed23n0032_6798", "title": "[Reconstruction of the lacrimal canaliculi (author's transl)].", "content": "The different methods of reconstructing obliterated lacrimal canaliculi are described. At the Eye Clinic in Erlangen Henderson's method is prefered. Into a new canal between the lacrimal punctum and the lacrimal sac a tube of polyethylene is introduced and remains there for some weeks. About 60% of the reconstruction lacrimal canaliculi were still open after months and years.", "contents": "[Reconstruction of the lacrimal canaliculi (author's transl)]. The different methods of reconstructing obliterated lacrimal canaliculi are described. At the Eye Clinic in Erlangen Henderson's method is prefered. Into a new canal between the lacrimal punctum and the lacrimal sac a tube of polyethylene is introduced and remains there for some weeks. About 60% of the reconstruction lacrimal canaliculi were still open after months and years.", "PMID": 966595}
{"id": "pubmed23n0032_6799", "title": "[Rare ocular manifestations of Boeck's sarcoidosis (author's transl)].", "content": "The clinical and histological diagnosis of two patients having an unusual localisation of eyelid and orbita with sarcoidosis Besnier-Boeck-Schaumann is explained. The importance of a prompt excission of the sample for the diagnosis and initiation of therapy is emphasized.", "contents": "[Rare ocular manifestations of Boeck's sarcoidosis (author's transl)]. The clinical and histological diagnosis of two patients having an unusual localisation of eyelid and orbita with sarcoidosis Besnier-Boeck-Schaumann is explained. The importance of a prompt excission of the sample for the diagnosis and initiation of therapy is emphasized.", "PMID": 966596}
{"id": "pubmed23n0032_6800", "title": "[Intraocular cryptococcosis (author's transl)].", "content": "A 79-year-old female patient suffered from iridocyclitis of both eyes. In the fundus of the left eye equatorially a preretinal questionable granuloma (size 1/2 d.d.) was found; the first hint of a mycotic involvement. In the enucleated right bulbus, an abscess-like granulomatous focus with a diameter of 10 mm was found. Several round encapsulated cells having a diameter of 7 to 20 mu and looking like with Cryptococcus neoformans were microscopically demonstrable in the pus obtained from this abscess. Since the fungus could not be cultured, it may be assumed that a spontaneous healing occurred. The only known basic disease was a temporary uremia (330 mg0/0 urea and 7.0 mg0/0 creatinin). The role of amphotericin B therapy in this patient is discussed.", "contents": "[Intraocular cryptococcosis (author's transl)]. A 79-year-old female patient suffered from iridocyclitis of both eyes. In the fundus of the left eye equatorially a preretinal questionable granuloma (size 1/2 d.d.) was found; the first hint of a mycotic involvement. In the enucleated right bulbus, an abscess-like granulomatous focus with a diameter of 10 mm was found. Several round encapsulated cells having a diameter of 7 to 20 mu and looking like with Cryptococcus neoformans were microscopically demonstrable in the pus obtained from this abscess. Since the fungus could not be cultured, it may be assumed that a spontaneous healing occurred. The only known basic disease was a temporary uremia (330 mg0/0 urea and 7.0 mg0/0 creatinin). The role of amphotericin B therapy in this patient is discussed.", "PMID": 966597}
{"id": "pubmed23n0032_6801", "title": "[Ophthalmic and orthoptic investigations in the kindergarten (author's transl)].", "content": "Recent observations of about 4000 children within the age of two to seven years are reported. It seems to be very useful to do all examinations such as visual acuity, cover-test and stereo-test in familial surroundings by experienced investigators. A survey of visual acuity, squint and stereopsis of all children is given in detail as well as an outline of the consequences of this study to the public health welfare.", "contents": "[Ophthalmic and orthoptic investigations in the kindergarten (author's transl)]. Recent observations of about 4000 children within the age of two to seven years are reported. It seems to be very useful to do all examinations such as visual acuity, cover-test and stereo-test in familial surroundings by experienced investigators. A survey of visual acuity, squint and stereopsis of all children is given in detail as well as an outline of the consequences of this study to the public health welfare.", "PMID": 966598}
{"id": "pubmed23n0032_6802", "title": "[The exfoliation syndrome: source of the fibrillar material on the capsule (author's transl)].", "content": "A clinical case of unilateral capsular exfoliation syndrome was observed in the previously traumatized eye of a 22-year-old man with a drawn-down pupil. The distribution of material corresponded to the folds of the inner surface of contiguous iris. The periphery of the lens was free of deposits, as far as could be seen. These findings contradict previous theories on the source of the fibrillar material from the lens epithelium and indicate that it is derived from the iris pigment epithelium and not from within the lens capsule, although there is no question that a similar process involves the peripheral lens epithelium and capsule, the products of which probably do not reach the lens surface.", "contents": "[The exfoliation syndrome: source of the fibrillar material on the capsule (author's transl)]. A clinical case of unilateral capsular exfoliation syndrome was observed in the previously traumatized eye of a 22-year-old man with a drawn-down pupil. The distribution of material corresponded to the folds of the inner surface of contiguous iris. The periphery of the lens was free of deposits, as far as could be seen. These findings contradict previous theories on the source of the fibrillar material from the lens epithelium and indicate that it is derived from the iris pigment epithelium and not from within the lens capsule, although there is no question that a similar process involves the peripheral lens epithelium and capsule, the products of which probably do not reach the lens surface.", "PMID": 966599}
{"id": "pubmed23n0032_6803", "title": "[Treatment of malignant lid tumours by intrapalpebral injection of urea (author's transl)].", "content": "36 malignant tumours of the eyelids (basaliomas, basal-cell carcinomas and squamous-cell carcinomas) have been treated by intrapalpebral injections of a 5% to 25% solution of urea as recommended by E.D. Danopoulos. 1.5 to 3.0 ml of the solutions were injected around the tumours twice per week. Six malignant tumours disappeared completely after a treatment of eleven months; their largest initial diameter averaged 0.53 cm. Considering the protracted treatment, the uncertainty of success, without histological examination of the excised scar tissue, and finally because of the low rate of verified success, injections of dissolved urea around malignant tumours of the eye lids cannot replace radical excision.", "contents": "[Treatment of malignant lid tumours by intrapalpebral injection of urea (author's transl)]. 36 malignant tumours of the eyelids (basaliomas, basal-cell carcinomas and squamous-cell carcinomas) have been treated by intrapalpebral injections of a 5% to 25% solution of urea as recommended by E.D. Danopoulos. 1.5 to 3.0 ml of the solutions were injected around the tumours twice per week. Six malignant tumours disappeared completely after a treatment of eleven months; their largest initial diameter averaged 0.53 cm. Considering the protracted treatment, the uncertainty of success, without histological examination of the excised scar tissue, and finally because of the low rate of verified success, injections of dissolved urea around malignant tumours of the eye lids cannot replace radical excision.", "PMID": 966600}
{"id": "pubmed23n0032_6804", "title": "[Eye involvement in plasmocytoma (author's transl)].", "content": "A description is given of a 37-year-old man, who suffered from a IgG myelom, diagnosed by internal medicine. The initial symptoms of the disease were grey-white, crystalline deposits in the cornea and recurring inflammation of the iris in both eyes. Only when a multiple recurring exophthalmos occurred, could the diagnosis be determined through an intensive internal examination. The continuing disease was still complicated by an insufficiency of the kidneys with hypertony and beginning retinopathia angiospastica.", "contents": "[Eye involvement in plasmocytoma (author's transl)]. A description is given of a 37-year-old man, who suffered from a IgG myelom, diagnosed by internal medicine. The initial symptoms of the disease were grey-white, crystalline deposits in the cornea and recurring inflammation of the iris in both eyes. Only when a multiple recurring exophthalmos occurred, could the diagnosis be determined through an intensive internal examination. The continuing disease was still complicated by an insufficiency of the kidneys with hypertony and beginning retinopathia angiospastica.", "PMID": 966601}
{"id": "pubmed23n0032_6805", "title": "[Micrometastasis of the uvea: a rare cause of uveal effusion (author's transl)].", "content": "A clinicopathological report is given on a case of spontaneous chorioideal detachment in a patient with metastatic carcinoma. Histological there was infiltration of the ciliary body and peripheral choroid and a large uveal effusion. Pain would seem to be a pathognomic sign of metastatic tumor in choroideal detachment caused by metastatic tumor.", "contents": "[Micrometastasis of the uvea: a rare cause of uveal effusion (author's transl)]. A clinicopathological report is given on a case of spontaneous chorioideal detachment in a patient with metastatic carcinoma. Histological there was infiltration of the ciliary body and peripheral choroid and a large uveal effusion. Pain would seem to be a pathognomic sign of metastatic tumor in choroideal detachment caused by metastatic tumor.", "PMID": 966602}
{"id": "pubmed23n0032_6806", "title": "[Electrolytic eye-lash epilation with the operating microscope (author's transl)].", "content": "A modified technique of epilation by electrolysis is reported. Epilation is hereby performed under the operating microscope. Contrary to the original method, with this technique the eyelash is firstly removed and then the hair canal is sounded with a fine wire under corresponding magnification. After the circuit is closed, white foam is formed and subsequently the hair papilla and the hair follicle are destroyed. The technique has several advantages compared to the original method: 1. It is relatively harmless to tissue. 2. Larger areas of abberant cilia may be epilated in one session as confluent necroses do not occur. 3. The risk of cilia again growing is reduced as the hair follicle and hair papilla are destroyed with high certainty.", "contents": "[Electrolytic eye-lash epilation with the operating microscope (author's transl)]. A modified technique of epilation by electrolysis is reported. Epilation is hereby performed under the operating microscope. Contrary to the original method, with this technique the eyelash is firstly removed and then the hair canal is sounded with a fine wire under corresponding magnification. After the circuit is closed, white foam is formed and subsequently the hair papilla and the hair follicle are destroyed. The technique has several advantages compared to the original method: 1. It is relatively harmless to tissue. 2. Larger areas of abberant cilia may be epilated in one session as confluent necroses do not occur. 3. The risk of cilia again growing is reduced as the hair follicle and hair papilla are destroyed with high certainty.", "PMID": 966603}
{"id": "pubmed23n0032_6807", "title": "[Spontaneous improvement of visual fusion by correction of minor anisometropia (author's transl)].", "content": "It is shown from two examples that spectacle correction of minor grades of anisometropia in children can be sufficient to allow them to fuse normally and to avoid asthenopic complaints caused by partial suppression of the image from one eye.", "contents": "[Spontaneous improvement of visual fusion by correction of minor anisometropia (author's transl)]. It is shown from two examples that spectacle correction of minor grades of anisometropia in children can be sufficient to allow them to fuse normally and to avoid asthenopic complaints caused by partial suppression of the image from one eye.", "PMID": 966604}
{"id": "pubmed23n0032_6808", "title": "[A comparative trial between pilocarpine drops and ocusert-pilocarpine].", "content": "Ocusert P 20/P 40 was tested on 53 outpatients with glaucoma for 5 weeks. In 27 patients good results and compatibility could be seen. The therapy had to be stopped with 26 patients, due to bad compatibility in 16 patients, and due to insufficient effectiveness in 10 patients.", "contents": "[A comparative trial between pilocarpine drops and ocusert-pilocarpine]. Ocusert P 20/P 40 was tested on 53 outpatients with glaucoma for 5 weeks. In 27 patients good results and compatibility could be seen. The therapy had to be stopped with 26 patients, due to bad compatibility in 16 patients, and due to insufficient effectiveness in 10 patients.", "PMID": 966605}
{"id": "pubmed23n0032_6809", "title": "[Pilocarpine as a diagnostic agent for early recognition of a tendency to glaucoma. (Comparable results between Hollwich's pilocarpine-test and tonography) (author's transl)].", "content": "In a group of 20 patients Hollwich's pilocarpine-test and tonography were performed repeatedly at regular intervals, for diagnostic purposes. The results of the pilocarpine-test were compared with those of tonography. Hence it was shown, that the pilocarpine-test performed at the peak of the daily pressure curve, gives comparable results to tonography and hence can be useful when glaucoma suspicion exists in the eye-doctor's surgery.", "contents": "[Pilocarpine as a diagnostic agent for early recognition of a tendency to glaucoma. (Comparable results between Hollwich's pilocarpine-test and tonography) (author's transl)]. In a group of 20 patients Hollwich's pilocarpine-test and tonography were performed repeatedly at regular intervals, for diagnostic purposes. The results of the pilocarpine-test were compared with those of tonography. Hence it was shown, that the pilocarpine-test performed at the peak of the daily pressure curve, gives comparable results to tonography and hence can be useful when glaucoma suspicion exists in the eye-doctor's surgery.", "PMID": 966606}
{"id": "pubmed23n0032_6810", "title": "[Ocular myiasis (author's transl)].", "content": "One case of an extraocular myiasis is presented. It is reported on parasites, which cause an ocular myiasis, complications and treatments.", "contents": "[Ocular myiasis (author's transl)]. One case of an extraocular myiasis is presented. It is reported on parasites, which cause an ocular myiasis, complications and treatments.", "PMID": 966607}
{"id": "pubmed23n0032_6811", "title": "[Retinopathy and maculopathy in cystinosis (author's transl)].", "content": "Retinopathy and maculopathy combined with pseudoneuritis were observed in cystinosis. The disease was classified as a nephropathic process.", "contents": "[Retinopathy and maculopathy in cystinosis (author's transl)]. Retinopathy and maculopathy combined with pseudoneuritis were observed in cystinosis. The disease was classified as a nephropathic process.", "PMID": 966608}
{"id": "pubmed23n0032_6812", "title": "[Self-destruction by repeated introduction of foreign bodies into the eye (author's transl)].", "content": "There is an observation of a 30-year-old woman inserting foreign bodies into the left eye. Perhaps a grave psychiatric sign", "contents": "[Self-destruction by repeated introduction of foreign bodies into the eye (author's transl)]. There is an observation of a 30-year-old woman inserting foreign bodies into the left eye. Perhaps a grave psychiatric sign", "PMID": 966609}
{"id": "pubmed23n0032_6813", "title": "[Ophthalmic-pharmacological complications (author's transl)].", "content": "The action of some drugs on the eye may lead to the occurrence of unforeseen ocular and extraocular reactions. Vincaleukoblastin (Velbe) provokes serious corneal lesions with a healing time of two weeks and a half. The local application of Neo-Synephrine eye drops (10%) and Mydrial-Atropine ointment may lead in the case of a pharmacogenetic disposition to disagreeable systemic effects. In one case of preparation for a cataract operation by akinesis and retrobulbar injection, Novocain was changed by mistake with Pilocarpinum hydrochloricum by the error of a pharmaceutical firm. This led to severe complications, which could by successfully treated with Chlorpromazine (Largactil). The knowledge of these clinical observations may be of greater usefulness in the preventation of untoward drug reactions.", "contents": "[Ophthalmic-pharmacological complications (author's transl)]. The action of some drugs on the eye may lead to the occurrence of unforeseen ocular and extraocular reactions. Vincaleukoblastin (Velbe) provokes serious corneal lesions with a healing time of two weeks and a half. The local application of Neo-Synephrine eye drops (10%) and Mydrial-Atropine ointment may lead in the case of a pharmacogenetic disposition to disagreeable systemic effects. In one case of preparation for a cataract operation by akinesis and retrobulbar injection, Novocain was changed by mistake with Pilocarpinum hydrochloricum by the error of a pharmaceutical firm. This led to severe complications, which could by successfully treated with Chlorpromazine (Largactil). The knowledge of these clinical observations may be of greater usefulness in the preventation of untoward drug reactions.", "PMID": 966610}
{"id": "pubmed23n0032_6814", "title": "[A soft, self-adhering contact lens for fundus examination (author's transl)].", "content": "A soft, self-adhering, biconcave silicone contact lens is presented. After surface anesthesia the lens can be placed on the cornea without additional contact fluid and a fundus area of about 35 degrees can be viewed. For vitreous surgery a modification with a flat or slightly convex surface is recommended.", "contents": "[A soft, self-adhering contact lens for fundus examination (author's transl)]. A soft, self-adhering, biconcave silicone contact lens is presented. After surface anesthesia the lens can be placed on the cornea without additional contact fluid and a fundus area of about 35 degrees can be viewed. For vitreous surgery a modification with a flat or slightly convex surface is recommended.", "PMID": 966611}
{"id": "pubmed23n0032_6815", "title": "[Retinoschisis and detachment (author's transl)].", "content": "Senile retinoschisis was found in 30 out of 315 pairs of eyes taken from cadavers. The retinal splitting occurred primarily as a result of the confluence of peripheral cystoid degeneration (Blessig's spaces or Iwanoff's cysts). There is no reason to suppose that two different forms of retinoschisis are present if the vessels are located in the inner layer. Based on reticular cystoid degeneration, there was only once case which could have represented another types of splitting. Here the vessels were located in the outer layer; the inner layer consisted only of the vitreous membrane. External spaces in the layer are the main causal factor for the development of retinoschisis detachment.", "contents": "[Retinoschisis and detachment (author's transl)]. Senile retinoschisis was found in 30 out of 315 pairs of eyes taken from cadavers. The retinal splitting occurred primarily as a result of the confluence of peripheral cystoid degeneration (Blessig's spaces or Iwanoff's cysts). There is no reason to suppose that two different forms of retinoschisis are present if the vessels are located in the inner layer. Based on reticular cystoid degeneration, there was only once case which could have represented another types of splitting. Here the vessels were located in the outer layer; the inner layer consisted only of the vitreous membrane. External spaces in the layer are the main causal factor for the development of retinoschisis detachment.", "PMID": 966612}
{"id": "pubmed23n0032_6816", "title": "[A-scan-ultrasonography in unilateral optic nerve lesions (author's transl)].", "content": "With A-scan-ultrasonography it is possible to determine the time of transmission of ultrasound within the cross section of the distal optic nerve. In this way information regarding its thickness can be obtained. 156 patients with various unilateral optic nerve lesions were examined. Thickening of the affected nerve was found in the acute phase of neuritis nervi optici, trauma, optic nerve tumors and in papilloedema due to raised orbital or intracranial pressure. This investigation has proved a valuable aid in diagnosis in the following unilateral conditions: 1. Acute visual loss in the absence of significant fundus findings--an enlarged optic nerve ultrasonogram is an objective sign of a retrobulbar neuritis, or after injury, a haematoma. 2. Papilloedema--the optic nerve ultrasonogram gives information about the nerve. 3. Optic atrophy--an enlarged optic nerve ultrasonogram indicates an optic nerve or sheath tumor.", "contents": "[A-scan-ultrasonography in unilateral optic nerve lesions (author's transl)]. With A-scan-ultrasonography it is possible to determine the time of transmission of ultrasound within the cross section of the distal optic nerve. In this way information regarding its thickness can be obtained. 156 patients with various unilateral optic nerve lesions were examined. Thickening of the affected nerve was found in the acute phase of neuritis nervi optici, trauma, optic nerve tumors and in papilloedema due to raised orbital or intracranial pressure. This investigation has proved a valuable aid in diagnosis in the following unilateral conditions: 1. Acute visual loss in the absence of significant fundus findings--an enlarged optic nerve ultrasonogram is an objective sign of a retrobulbar neuritis, or after injury, a haematoma. 2. Papilloedema--the optic nerve ultrasonogram gives information about the nerve. 3. Optic atrophy--an enlarged optic nerve ultrasonogram indicates an optic nerve or sheath tumor.", "PMID": 966613}
{"id": "pubmed23n0032_6817", "title": "[Heterotopic fundus (author's transl)].", "content": "Fundus heterotopicus is the term used to describe a rare, non-hereditary curvature anomaly of the fundus in the non-myopic eye, which is characterized: 1. functionally, by a slowly increasing myopic-astigmatic refractive error, 2. by correctable bitemporal or binasal refractionscomata and 3. ophthalmoscopically by a posterior out-pouching of the nasal or temporal fundus portions, and including the optic disc and macula in the obliquely descending wall of the extasis.", "contents": "[Heterotopic fundus (author's transl)]. Fundus heterotopicus is the term used to describe a rare, non-hereditary curvature anomaly of the fundus in the non-myopic eye, which is characterized: 1. functionally, by a slowly increasing myopic-astigmatic refractive error, 2. by correctable bitemporal or binasal refractionscomata and 3. ophthalmoscopically by a posterior out-pouching of the nasal or temporal fundus portions, and including the optic disc and macula in the obliquely descending wall of the extasis.", "PMID": 966614}
{"id": "pubmed23n0032_6818", "title": "[Traumatic palsies of the inferior oblique muscle (author's transl)].", "content": "The author discussed the increase in the frequency of traumatic paralyses of the ocular muscles, and reported 6 cases of inferior oblique muscle paresis caused by local blunt or sharp traumas (haematoma, contusion, rupture, incarceration) and one case of traumatic Brownian pseudo-paralysis. The localisation of the injuries in 3 cases was on the inferior temporal part and in the other 3 cases on the superior part of the bulbar conjunctiva. Atypical horizontal deviation and characteristic vertical deviation, torsion and torticollis were observed in the majority of cases. One patient recovered spontaneously. 3 patients became asymptomatic after correction by prisms and one after recession of contralateral superior rectus muscle. The symptoms remained unchanged in the case of a \"blow out\" fracture (in spite of operation) and a Brown's syndrome (without treatment).", "contents": "[Traumatic palsies of the inferior oblique muscle (author's transl)]. The author discussed the increase in the frequency of traumatic paralyses of the ocular muscles, and reported 6 cases of inferior oblique muscle paresis caused by local blunt or sharp traumas (haematoma, contusion, rupture, incarceration) and one case of traumatic Brownian pseudo-paralysis. The localisation of the injuries in 3 cases was on the inferior temporal part and in the other 3 cases on the superior part of the bulbar conjunctiva. Atypical horizontal deviation and characteristic vertical deviation, torsion and torticollis were observed in the majority of cases. One patient recovered spontaneously. 3 patients became asymptomatic after correction by prisms and one after recession of contralateral superior rectus muscle. The symptoms remained unchanged in the case of a \"blow out\" fracture (in spite of operation) and a Brown's syndrome (without treatment).", "PMID": 966615}
{"id": "pubmed23n0032_6819", "title": "[Aberrant reinnervation after oculomotor palsy with involvement of the ciliary muscle (author's transl)].", "content": "The clinical and electromyographic signs of the misdirection syndrome after oculomotor palsy are described. Besides the previously described findings we found an increase of intraocular pressure dependent on the direction of gaze. In three young patients (16-26 years), we were able to demonstrate an aberrant reinnervation of the ciliary muscle, which was responsible for an increasing myopia in adduction. These observations supplement the previous concept of the misdirection syndrome.", "contents": "[Aberrant reinnervation after oculomotor palsy with involvement of the ciliary muscle (author's transl)]. The clinical and electromyographic signs of the misdirection syndrome after oculomotor palsy are described. Besides the previously described findings we found an increase of intraocular pressure dependent on the direction of gaze. In three young patients (16-26 years), we were able to demonstrate an aberrant reinnervation of the ciliary muscle, which was responsible for an increasing myopia in adduction. These observations supplement the previous concept of the misdirection syndrome.", "PMID": 966616}
{"id": "pubmed23n0032_6820", "title": "[Radionuclide dacryography in clinical practice and research (author's transl)].", "content": "Radionuclide dacryography (RND) has the following indications: 1. Localisation of obstructions in the lacrimal drainage system. 2. Judgement of relative tear obstruction. 3. Pre- and post-operative control of the function of lacrimal drainage in surgical and radiotherapy of the inner canthus region. 4. Investigations of tear secretion are described and own preliminary results are discussed. 5. Resorption of suitable marker substances clinically. First results of conjunctival resorption of pertechnetate are reported. 6. The possibility of testing in vivo radioactive drug delivery systems is shown by means of own investigations. 7. The absorbed radiation dose using RND is very low as compared to radiological methods, thus justifying repeated controls and investigations even in children.", "contents": "[Radionuclide dacryography in clinical practice and research (author's transl)]. Radionuclide dacryography (RND) has the following indications: 1. Localisation of obstructions in the lacrimal drainage system. 2. Judgement of relative tear obstruction. 3. Pre- and post-operative control of the function of lacrimal drainage in surgical and radiotherapy of the inner canthus region. 4. Investigations of tear secretion are described and own preliminary results are discussed. 5. Resorption of suitable marker substances clinically. First results of conjunctival resorption of pertechnetate are reported. 6. The possibility of testing in vivo radioactive drug delivery systems is shown by means of own investigations. 7. The absorbed radiation dose using RND is very low as compared to radiological methods, thus justifying repeated controls and investigations even in children.", "PMID": 966617}
{"id": "pubmed23n0032_6821", "title": "[Intraarterial injection of Tolazolinhydrochlorid in retinal arterial occlusions. Our experiences with puncture of the carotid artery (author's transl)].", "content": "In partial and total retinal arterial occlusions we have been giving intracarotideal Tolazolinhydrochlorid since 1969. In the beginning we have prepared surgically the common carotid artery, at present this puncture is done percutanously. The author describes the surgical procedure and the results. A significant improvement of the visual acuity and of the visual field was present in the cases with intraarterial injection.", "contents": "[Intraarterial injection of Tolazolinhydrochlorid in retinal arterial occlusions. Our experiences with puncture of the carotid artery (author's transl)]. In partial and total retinal arterial occlusions we have been giving intracarotideal Tolazolinhydrochlorid since 1969. In the beginning we have prepared surgically the common carotid artery, at present this puncture is done percutanously. The author describes the surgical procedure and the results. A significant improvement of the visual acuity and of the visual field was present in the cases with intraarterial injection.", "PMID": 966618}
{"id": "pubmed23n0032_6822", "title": "[Studies on the changes of transcortin-level in patients with glaucoma (author's transl)].", "content": "Changes in the plasma transcortin level were studied in patients with primary glaucoma, glaucoma and diabetes, diabetes mellitus compared with a control croup. It was found that the plasma transcortin level of the patients with or without glaucoma was significantly higher than that of the control values. Moreover, the analysis of the dissociation constant of transcortincorticosterone complex revealed that it is greater in patients with primary glaucoma than could be expected by the dilution ratio. Further studies are required to understand the mechanism of the changes occurring in plasma transcortin level in glaucoma.", "contents": "[Studies on the changes of transcortin-level in patients with glaucoma (author's transl)]. Changes in the plasma transcortin level were studied in patients with primary glaucoma, glaucoma and diabetes, diabetes mellitus compared with a control croup. It was found that the plasma transcortin level of the patients with or without glaucoma was significantly higher than that of the control values. Moreover, the analysis of the dissociation constant of transcortincorticosterone complex revealed that it is greater in patients with primary glaucoma than could be expected by the dilution ratio. Further studies are required to understand the mechanism of the changes occurring in plasma transcortin level in glaucoma.", "PMID": 966619}
{"id": "pubmed23n0032_6823", "title": "[Relationships between hemopoiesis and peripheral blood counts in untreated and 32P-treated patients with polycythaemia vera (author's transl)].", "content": "The composition of the hemopoiesis was determined in iliac crest biopsies of 51 patients with polycythaemia vera. There was a good correlation between thrombopoiesis and thrombocytes and, to a minor degree also between erythropoiesis and erythrocytes in untreated patients as well as between granulopoiesis and granulocytes in 32P-treated patients. In patients with normal blood counts there existed no correlation between the bone marrow and the blood counts within smaller limits of the cell count. Histomorphometric analysis shows no difference either between untreated and 32P-treated patients or between patients with and without splenomegaly. This is an argument against a significant intrasplenic hemopoiesis or an intrasplenic cell pooling, or destruction (hypersplenism), respectively. The blood sinusoids are hyperplastic and distended. With increasing hyperplasia of the hemopoiesis the sinusoids become relatively smaller. So changes in vascularisation may be of importance in the infrequent transitions into myelofibrosis and/or leukemia.", "contents": "[Relationships between hemopoiesis and peripheral blood counts in untreated and 32P-treated patients with polycythaemia vera (author's transl)]. The composition of the hemopoiesis was determined in iliac crest biopsies of 51 patients with polycythaemia vera. There was a good correlation between thrombopoiesis and thrombocytes and, to a minor degree also between erythropoiesis and erythrocytes in untreated patients as well as between granulopoiesis and granulocytes in 32P-treated patients. In patients with normal blood counts there existed no correlation between the bone marrow and the blood counts within smaller limits of the cell count. Histomorphometric analysis shows no difference either between untreated and 32P-treated patients or between patients with and without splenomegaly. This is an argument against a significant intrasplenic hemopoiesis or an intrasplenic cell pooling, or destruction (hypersplenism), respectively. The blood sinusoids are hyperplastic and distended. With increasing hyperplasia of the hemopoiesis the sinusoids become relatively smaller. So changes in vascularisation may be of importance in the infrequent transitions into myelofibrosis and/or leukemia.", "PMID": 966631}
{"id": "pubmed23n0032_6824", "title": "[Insufficiency of the exocrine pancreas in Wilson's disease (author's transl)].", "content": "The function of the exocrine pancreas was examined by the secretin-pancreozymin-test in 3 patients with Wilson's disease. In all cases we found a partial insufficiency. At the time of investigation the patients were 6(7)/12, 11(6)/12 and 21 years old. The youngest one was examined before therapy with D-Penicillamin. We suppose that storage of copper in lysosomes causes a cytotoxic damage of the exocrine part of the pancreas requiring substitution therapy in advanced cases.", "contents": "[Insufficiency of the exocrine pancreas in Wilson's disease (author's transl)]. The function of the exocrine pancreas was examined by the secretin-pancreozymin-test in 3 patients with Wilson's disease. In all cases we found a partial insufficiency. At the time of investigation the patients were 6(7)/12, 11(6)/12 and 21 years old. The youngest one was examined before therapy with D-Penicillamin. We suppose that storage of copper in lysosomes causes a cytotoxic damage of the exocrine part of the pancreas requiring substitution therapy in advanced cases.", "PMID": 966633}
{"id": "pubmed23n0032_6825", "title": "[Changes of erythrocyte membrane lipids in ethanol induced hyperlipidemia (Zieve's syndrome) (author's transl)].", "content": "In 11 patients with alcohol-induced hyperlipemia, of whom 6 showed a Zieve Syndrome increased phospholipids, triglycerides and total cholesterol were found in the red cells stromal. The gasliquid chromatographic analysis of the phospholipid fatty acids showed increased contents of saturated and monounsaturated fatty acids C 16-C 18 smaller contents of longchain highly unsaturated fatty acids. The changes in the fatty acid pattern mainly occurred in patients with Zieve Syndrome and could be important for the mechanism of the hemolytic anemia. Theses findings could be in connexion with vitamin E deficiency.", "contents": "[Changes of erythrocyte membrane lipids in ethanol induced hyperlipidemia (Zieve's syndrome) (author's transl)]. In 11 patients with alcohol-induced hyperlipemia, of whom 6 showed a Zieve Syndrome increased phospholipids, triglycerides and total cholesterol were found in the red cells stromal. The gasliquid chromatographic analysis of the phospholipid fatty acids showed increased contents of saturated and monounsaturated fatty acids C 16-C 18 smaller contents of longchain highly unsaturated fatty acids. The changes in the fatty acid pattern mainly occurred in patients with Zieve Syndrome and could be important for the mechanism of the hemolytic anemia. Theses findings could be in connexion with vitamin E deficiency.", "PMID": 966632}
{"id": "pubmed23n0032_6826", "title": "Biliary reabsorption of cholate sodium, glycocholate sodium, and taurocholate sodium from the rat biliary tree after retrograde intrabiliary injection.", "content": "The results demonstrate the biliary reabsorption of 14C-cholate, 14C-glycocholate, and 14C-taurocholate from the rat biliary tree after retrograde intrabiliary injection. It could be shown that retrograde injection of these bile salts (20 nmol) in a total volume of 40 mul leads to significantly increased biliary reabsorption in contrast to the administration in a retrograde volume of only 20 mul. These differences in reabsorption may be explained by greater reabsorption at a more proximal site in the biliary tree. Furthermore 14C-glycocholate and 14C-taurocholate are reabsorbed to a lesser extent in contrast to 14C-cholate when bile flow was restarted at once after retrograde injection in a volume of 40 mul. It is speculated that conjugation of cholate to glycine and taurine has some effect on the extent of biliary reabsorption of this bile acid. Following the results presented in this paper one might hypothize that biliary reabsorption has an important influence on bile composition i.e. the biliary excretion of bile salts.", "contents": "Biliary reabsorption of cholate sodium, glycocholate sodium, and taurocholate sodium from the rat biliary tree after retrograde intrabiliary injection. The results demonstrate the biliary reabsorption of 14C-cholate, 14C-glycocholate, and 14C-taurocholate from the rat biliary tree after retrograde intrabiliary injection. It could be shown that retrograde injection of these bile salts (20 nmol) in a total volume of 40 mul leads to significantly increased biliary reabsorption in contrast to the administration in a retrograde volume of only 20 mul. These differences in reabsorption may be explained by greater reabsorption at a more proximal site in the biliary tree. Furthermore 14C-glycocholate and 14C-taurocholate are reabsorbed to a lesser extent in contrast to 14C-cholate when bile flow was restarted at once after retrograde injection in a volume of 40 mul. It is speculated that conjugation of cholate to glycine and taurine has some effect on the extent of biliary reabsorption of this bile acid. Following the results presented in this paper one might hypothize that biliary reabsorption has an important influence on bile composition i.e. the biliary excretion of bile salts.", "PMID": 966634}
{"id": "pubmed23n0032_6827", "title": "Lymphatic absorption and metabolism of orally administered testosterone undecanoate in man.", "content": "[3H]-testosterone undecanoate ([3H]TU) was administered orally to 4 patients with a thoracic duct catheter after neck dissection surgery. Appearance of radioactivity in lymph, plasma and urine was measured at different times. Metabolites of TU in these fluids were investigated. Peak levels of radioactivity appeared simultaneously in lymph and plasma (2.5-5 h after administration) while the excretion in urine was highest approximately 2 h after the plasma and lymph peak. The main compounds appearing in the lymph were TU and 5alpha-dihydrotestosterone undecanoate (5alpha-DHTU), but 5beta-DHTU could not be detected. In plasma almost all metabolites were probably conjugated. During the first 24 h approximately 40% of the administered radioactivity was excreted in the urine. The total amount of radioactivity excreted in the urine during the first week was 45-48%. The predominant urinary metabolites were testosterone- and androsterone-glucuronide. The results indicate that TU is metabolized partly in the intestinal wall. The remaining TU and newly-formed 5alpha-DHTU, at least partly, are absorbed via the lymphatic system.", "contents": "Lymphatic absorption and metabolism of orally administered testosterone undecanoate in man. [3H]-testosterone undecanoate ([3H]TU) was administered orally to 4 patients with a thoracic duct catheter after neck dissection surgery. Appearance of radioactivity in lymph, plasma and urine was measured at different times. Metabolites of TU in these fluids were investigated. Peak levels of radioactivity appeared simultaneously in lymph and plasma (2.5-5 h after administration) while the excretion in urine was highest approximately 2 h after the plasma and lymph peak. The main compounds appearing in the lymph were TU and 5alpha-dihydrotestosterone undecanoate (5alpha-DHTU), but 5beta-DHTU could not be detected. In plasma almost all metabolites were probably conjugated. During the first 24 h approximately 40% of the administered radioactivity was excreted in the urine. The total amount of radioactivity excreted in the urine during the first week was 45-48%. The predominant urinary metabolites were testosterone- and androsterone-glucuronide. The results indicate that TU is metabolized partly in the intestinal wall. The remaining TU and newly-formed 5alpha-DHTU, at least partly, are absorbed via the lymphatic system.", "PMID": 966635}
{"id": "pubmed23n0032_6828", "title": "[Decrease of disaccharidase activity following application of bencyclan-hydrogenfumarate (author's transl)].", "content": "The disaccharidase activity of the small bowel mucosa of the rat is decreased by intraperitoneal application of Bencyclan-hydrogenfumarate. The doses applied were 10, 50 and 100 mg/kg body weight. The decrease rate after 100 mg/kg attained up to 50% of the control values. Furthermore, a reduction of villous height and an increase of the crypt depth has been observed in correlation with the enzyme activity decrease. Though functional and morphological alterations show a correlation, they cannot be explained by an antimetabolic-like effect because of the fact that the enzyme function recovers 72 h after application of the drug. Whilst our results do not sustain a cytostatic effect of Bencyclan-hydrogenfumarate, there is no doubt that the intraperitoneally applied drug influences the disaccharidase activity. These facts have to be considered in clinical therapy.", "contents": "[Decrease of disaccharidase activity following application of bencyclan-hydrogenfumarate (author's transl)]. The disaccharidase activity of the small bowel mucosa of the rat is decreased by intraperitoneal application of Bencyclan-hydrogenfumarate. The doses applied were 10, 50 and 100 mg/kg body weight. The decrease rate after 100 mg/kg attained up to 50% of the control values. Furthermore, a reduction of villous height and an increase of the crypt depth has been observed in correlation with the enzyme activity decrease. Though functional and morphological alterations show a correlation, they cannot be explained by an antimetabolic-like effect because of the fact that the enzyme function recovers 72 h after application of the drug. Whilst our results do not sustain a cytostatic effect of Bencyclan-hydrogenfumarate, there is no doubt that the intraperitoneally applied drug influences the disaccharidase activity. These facts have to be considered in clinical therapy.", "PMID": 966636}
{"id": "pubmed23n0032_6829", "title": "[DNA depolymerase activity in rat spleen following flight on the \"Kosmos-605= satellite].", "content": "The activity of enzymes involved in DNA disintegration (acid and alkaline DNA-ases) was measured in the spleen of rats flown onboard the Cosmos-605 biosatellite for 22 days. The activity of alkaline DNA-ase remained unaltered whereas that of acid DNA-ase increased more than twice. The activity of acid DNA-ase returned to the normal 26 days postflight. The study of characteristic viscosity (eta) of native and denatured DNA showed that enzyme activation was not followed by degradation changes in the DNA molecule.", "contents": "[DNA depolymerase activity in rat spleen following flight on the \"Kosmos-605= satellite]. The activity of enzymes involved in DNA disintegration (acid and alkaline DNA-ases) was measured in the spleen of rats flown onboard the Cosmos-605 biosatellite for 22 days. The activity of alkaline DNA-ase remained unaltered whereas that of acid DNA-ase increased more than twice. The activity of acid DNA-ase returned to the normal 26 days postflight. The study of characteristic viscosity (eta) of native and denatured DNA showed that enzyme activation was not followed by degradation changes in the DNA molecule.", "PMID": 966671}
{"id": "pubmed23n0032_6830", "title": "[Changes in human monosynaptic H-reflex during altitude decompression].", "content": "Test subjects exposed to altitude decompression without preliminary denitrogenization showed in 65-75% of cases stability or reduction of the amplitude of the H-response of a leg with subsequent painful sensations and in increase of the amplitude of the H-response in the other leg as long as they were raised to an altitude of 11,000 m. When bends developed in a leg the amplitude of the H-response in that leg decreased and in the opposite leg increased. During the descent and disappearance of painful sensations changes in the amplitude of the H-response were just the opposite. It is assumed that a decrease of the amplitude of the H-response during altitude decompression was associated with oxygen deficiency of nerve afferents and an increase--with the descending activating supraspinal effect.", "contents": "[Changes in human monosynaptic H-reflex during altitude decompression]. Test subjects exposed to altitude decompression without preliminary denitrogenization showed in 65-75% of cases stability or reduction of the amplitude of the H-response of a leg with subsequent painful sensations and in increase of the amplitude of the H-response in the other leg as long as they were raised to an altitude of 11,000 m. When bends developed in a leg the amplitude of the H-response in that leg decreased and in the opposite leg increased. During the descent and disappearance of painful sensations changes in the amplitude of the H-response were just the opposite. It is assumed that a decrease of the amplitude of the H-response during altitude decompression was associated with oxygen deficiency of nerve afferents and an increase--with the descending activating supraspinal effect.", "PMID": 966672}
{"id": "pubmed23n0032_6831", "title": "[State of the human motor apparatus during prolonged exposure to a nitrogen-oxygen atmosphere at a pressure of 5 atm].", "content": "The state of the motor function of individuals who were pedalling a bicycle ergometer in an altitude chamber containing a nitrogen-oxygen atmosphere at 5.0 ata was studied. Maximum isometric force, precision of controlling movement and muscular tension, accuracy and velocity of movements were recorded. The maximum force, precision and velocity of movements varied phasically. The greatest changes were found on the 1st-3rd and 6--7th days of the exposure. These parameters returned to the normal when the atmosphere pressure was reduced to 1.0 ata. During exercises at an elevated pressure the muscular force, accuracy and velocity of movements decreased. During exercises at a normal pressure similar changes were less distinct and consistent.", "contents": "[State of the human motor apparatus during prolonged exposure to a nitrogen-oxygen atmosphere at a pressure of 5 atm]. The state of the motor function of individuals who were pedalling a bicycle ergometer in an altitude chamber containing a nitrogen-oxygen atmosphere at 5.0 ata was studied. Maximum isometric force, precision of controlling movement and muscular tension, accuracy and velocity of movements were recorded. The maximum force, precision and velocity of movements varied phasically. The greatest changes were found on the 1st-3rd and 6--7th days of the exposure. These parameters returned to the normal when the atmosphere pressure was reduced to 1.0 ata. During exercises at an elevated pressure the muscular force, accuracy and velocity of movements decreased. During exercises at a normal pressure similar changes were less distinct and consistent.", "PMID": 966674}
{"id": "pubmed23n0032_6832", "title": "[Effect on water metabolism of body posture relative to the gravity vector].", "content": "A combined effect of hypokinesia and body position at different angles to the gravity vector on the water content and rate of its metabolism was studied. It was shown that bed rest reduced total water losses and the rate of its metabolism. It was body position as related to the gravity vector rather than bed rest that influenced the total water content in the body.", "contents": "[Effect on water metabolism of body posture relative to the gravity vector]. A combined effect of hypokinesia and body position at different angles to the gravity vector on the water content and rate of its metabolism was studied. It was shown that bed rest reduced total water losses and the rate of its metabolism. It was body position as related to the gravity vector rather than bed rest that influenced the total water content in the body.", "PMID": 966676}
{"id": "pubmed23n0032_6833", "title": "[Recovery of muscle functions in rats following prolonged movement restraint].", "content": "Experiments were carried out to measure time and force parameters of isometric contractions and structural parameters of muscles of hind limbs of rats which were exposed to hypokinesia for 130 days and then were kept under surveillance for 3 months. During recovery certain functions of the contractile system returned to the normal. At the same time M. soleus displayed persistent changes that were indicative of atrophic developments. Unlike M. soleus where delayed contraction (due to an increase in the time of relaxation) was followed by losses in force, fast muscles (M. ext. digit. longus and M. plantaris) showed recovery of force parameters. They also exhibited delayed contraction due to an increase in the time of tension development and half-relaxation. These differences are associated with dissimilar function and structure of muscular fibers. On the whole, the pattern of recovery of the motor functions reflects responses to an increased load of the neuromuscular system of animals which are kept in large cages after prolonged hypokinesia.", "contents": "[Recovery of muscle functions in rats following prolonged movement restraint]. Experiments were carried out to measure time and force parameters of isometric contractions and structural parameters of muscles of hind limbs of rats which were exposed to hypokinesia for 130 days and then were kept under surveillance for 3 months. During recovery certain functions of the contractile system returned to the normal. At the same time M. soleus displayed persistent changes that were indicative of atrophic developments. Unlike M. soleus where delayed contraction (due to an increase in the time of relaxation) was followed by losses in force, fast muscles (M. ext. digit. longus and M. plantaris) showed recovery of force parameters. They also exhibited delayed contraction due to an increase in the time of tension development and half-relaxation. These differences are associated with dissimilar function and structure of muscular fibers. On the whole, the pattern of recovery of the motor functions reflects responses to an increased load of the neuromuscular system of animals which are kept in large cages after prolonged hypokinesia.", "PMID": 966677}
{"id": "pubmed23n0032_6834", "title": "[Prevention of decompression sickness as applied to conditions of multiple space walks].", "content": "Six 3-day experiments were carried out in an altitude chamber in which 6 test subjects took part. The experiments simulated repeated extra-vehicular activities--intensive work at an altitude of 10,000 m in a helmet or at an altitude of 40,000 m on a space suit pressurized at 200 mm Hg. It was demonstrated that altitude decompression could be prevented by desaturation--prolonged exposure to the hypobaric atmosphere (430 mm Hg) and 100% O2 or a 40% O2 and 60% N2 mixture. During the 3-day experiment the test subjects made seven 3--4 hour excursions, showing no symptoms of decompression disease.", "contents": "[Prevention of decompression sickness as applied to conditions of multiple space walks]. Six 3-day experiments were carried out in an altitude chamber in which 6 test subjects took part. The experiments simulated repeated extra-vehicular activities--intensive work at an altitude of 10,000 m in a helmet or at an altitude of 40,000 m on a space suit pressurized at 200 mm Hg. It was demonstrated that altitude decompression could be prevented by desaturation--prolonged exposure to the hypobaric atmosphere (430 mm Hg) and 100% O2 or a 40% O2 and 60% N2 mixture. During the 3-day experiment the test subjects made seven 3--4 hour excursions, showing no symptoms of decompression disease.", "PMID": 966673}
{"id": "pubmed23n0032_6835", "title": "[Human adrenal function during prolonged exposure to hyperbaric conditions (N2-02, 5 atm)].", "content": "Test subjects were exposed to a nitrogen-oxygen atmosphere at 5 ata, performing various activities. The adrenal function of the subjects was examined with respect to the catecholamine and corticosteroids excretion in the urine. During adaptation to the environment the excretion of catecholamines and corticosteroids varied phasically. After 3 days of the 7-day exposure to the hyperbaric atmosphere and during decompression the excretion of norepinephrine increased and that of corticosteroids, particularly 17-corticosteroids, decreased. The diurnal rhythm of the excretion changed though the life regimen of the test subjects remained unaltered. These variations can be considered as adaptive-protective responses of the human body.", "contents": "[Human adrenal function during prolonged exposure to hyperbaric conditions (N2-02, 5 atm)]. Test subjects were exposed to a nitrogen-oxygen atmosphere at 5 ata, performing various activities. The adrenal function of the subjects was examined with respect to the catecholamine and corticosteroids excretion in the urine. During adaptation to the environment the excretion of catecholamines and corticosteroids varied phasically. After 3 days of the 7-day exposure to the hyperbaric atmosphere and during decompression the excretion of norepinephrine increased and that of corticosteroids, particularly 17-corticosteroids, decreased. The diurnal rhythm of the excretion changed though the life regimen of the test subjects remained unaltered. These variations can be considered as adaptive-protective responses of the human body.", "PMID": 966675}
{"id": "pubmed23n0032_6836", "title": "[Comparative characteristics of electrocardiograms of overweight and normal weight flight personnel in conditions of moderate hypoxia].", "content": "Examination of electrocardiograms of pilots with normal and 10% excessive weight in a moderately hypoxic atmosphere showed that changes in the pulse rate, T voltage, systolic index were less expressed in obese subjects than in normal pilots. This can be attributed to the fact that obese subjects had reduced metabolism, minute oxygen consumption and compensatory capabilities of the cardiovascular system.", "contents": "[Comparative characteristics of electrocardiograms of overweight and normal weight flight personnel in conditions of moderate hypoxia]. Examination of electrocardiograms of pilots with normal and 10% excessive weight in a moderately hypoxic atmosphere showed that changes in the pulse rate, T voltage, systolic index were less expressed in obese subjects than in normal pilots. This can be attributed to the fact that obese subjects had reduced metabolism, minute oxygen consumption and compensatory capabilities of the cardiovascular system.", "PMID": 966681}
{"id": "pubmed23n0032_6837", "title": "[Relation of human weight loss to the duration of space flight].", "content": "A formula describing weight losses in the first approximation during space mission was derived assuming that humans may adapt to space flight effects. The process depended on two parameters one of which characterized maximum body losses and another--time of adaptation. Weight estimates of Skylab crewmembers obtained with the aid of the formula were in good agreement with actual results. The typical time of adaptation was two days. Possible ways of constructing a more precise model of weight changes and its use to diagnose the status of crewmembers are discussed.", "contents": "[Relation of human weight loss to the duration of space flight]. A formula describing weight losses in the first approximation during space mission was derived assuming that humans may adapt to space flight effects. The process depended on two parameters one of which characterized maximum body losses and another--time of adaptation. Weight estimates of Skylab crewmembers obtained with the aid of the formula were in good agreement with actual results. The typical time of adaptation was two days. Possible ways of constructing a more precise model of weight changes and its use to diagnose the status of crewmembers are discussed.", "PMID": 966680}
{"id": "pubmed23n0032_6838", "title": "[Method of studying acceleration tolerance of pilots in flight conditions].", "content": "The following procedure was used to study the pilot's tolerance to acceleration inflight. Experiments were carried out in a specially equipped plane with a dual monitoring system. Inflight the pilot performed 3 dives, attaining 3.0, 5.0 and 6.0 g for 30 sec at an interval of 1-2 min and an onset rate of 0.4-0.6 g/sec. The control over the health state and performance of the pilot was maintained by the coach as well as with the aid of breathing rate, ECG, ear pulse rate recordings and cynematography of the pilot's face during exposure to acceleration. These methods were employed in experimental flights of a good number of pilots. The results show that the methods may well be used to better medical monitoring inflight and to identify subjects with decreased tolerance to acceleration.", "contents": "[Method of studying acceleration tolerance of pilots in flight conditions]. The following procedure was used to study the pilot's tolerance to acceleration inflight. Experiments were carried out in a specially equipped plane with a dual monitoring system. Inflight the pilot performed 3 dives, attaining 3.0, 5.0 and 6.0 g for 30 sec at an interval of 1-2 min and an onset rate of 0.4-0.6 g/sec. The control over the health state and performance of the pilot was maintained by the coach as well as with the aid of breathing rate, ECG, ear pulse rate recordings and cynematography of the pilot's face during exposure to acceleration. These methods were employed in experimental flights of a good number of pilots. The results show that the methods may well be used to better medical monitoring inflight and to identify subjects with decreased tolerance to acceleration.", "PMID": 966682}
{"id": "pubmed23n0032_6839", "title": "[Mouse hematopoietic organs in hypokinesia].", "content": "The capacity of hemopoietic cells for colony-formation and the pattern of their differentiation during hypokinesia were studied. The absolute count of colony-forming units (CFU) decreased exponentially during 3 to 30 days. Bone marrow showed a change in the total cell count and a wave-like change in the absolute and relative count of CFU with a maximum on the 1st-3rd and 15th-45th days. Differentiating potentialities of CFU from the spleen and bone marrow were not altered towards erythro-, myelo- and thrombopoiesis. The cell structure of the peripheral blood changed, displaying lymphopenia, transient neutrophil leukocytosis, increase in the percentage content of large lymphocytes and stress-lymphocytes.", "contents": "[Mouse hematopoietic organs in hypokinesia]. The capacity of hemopoietic cells for colony-formation and the pattern of their differentiation during hypokinesia were studied. The absolute count of colony-forming units (CFU) decreased exponentially during 3 to 30 days. Bone marrow showed a change in the total cell count and a wave-like change in the absolute and relative count of CFU with a maximum on the 1st-3rd and 15th-45th days. Differentiating potentialities of CFU from the spleen and bone marrow were not altered towards erythro-, myelo- and thrombopoiesis. The cell structure of the peripheral blood changed, displaying lymphopenia, transient neutrophil leukocytosis, increase in the percentage content of large lymphocytes and stress-lymphocytes.", "PMID": 966678}
{"id": "pubmed23n0032_6840", "title": "[Recovery of central vision after temporary blindness].", "content": "Recovery of spatial resolving capacity (acuity) of central vision after temporary blindness induced by prolonged (1.5, 3, 6 min) light adaptation to a sun or incandescence lamp (20, 40, 80 thous. lux) illuminated white screen was studied. The recovery time increased exponentially with an increase in energy light stimulation (product of brightness of the deadapting source by the time of action). A general formula describing the relationship between the time of recovery of acuity and brightness of the test table and energy of light stimulus was derived.", "contents": "[Recovery of central vision after temporary blindness]. Recovery of spatial resolving capacity (acuity) of central vision after temporary blindness induced by prolonged (1.5, 3, 6 min) light adaptation to a sun or incandescence lamp (20, 40, 80 thous. lux) illuminated white screen was studied. The recovery time increased exponentially with an increase in energy light stimulation (product of brightness of the deadapting source by the time of action). A general formula describing the relationship between the time of recovery of acuity and brightness of the test table and energy of light stimulus was derived.", "PMID": 966679}
{"id": "pubmed23n0032_6841", "title": "[Rabbit coronary vessels following a single irradiation of the heart region with a dose of 2400 rad].", "content": "The effect of ionizing radiation on the coronary vessels was studied on rabbits whose cardiac areas were irradiated at a dose of 2400 rads. This exposure led to dilatation and stricture of small vessels. The most significant changes were found in the system of the left coronary artery at an early post-irradiation period.", "contents": "[Rabbit coronary vessels following a single irradiation of the heart region with a dose of 2400 rad]. The effect of ionizing radiation on the coronary vessels was studied on rabbits whose cardiac areas were irradiated at a dose of 2400 rads. This exposure led to dilatation and stricture of small vessels. The most significant changes were found in the system of the left coronary artery at an early post-irradiation period.", "PMID": 966684}
{"id": "pubmed23n0032_6842", "title": "[Evaluation and application of quantitative indices of the work quality of an operator].", "content": "The paper gives assessment of quantitative parameters of the quality of the operators' activity involved in the spacecraft control. It discusses specific problems that can be solved using parameters characterizing the quality of the operator's activity during space flight, spacecraft development and operators' training.", "contents": "[Evaluation and application of quantitative indices of the work quality of an operator]. The paper gives assessment of quantitative parameters of the quality of the operators' activity involved in the spacecraft control. It discusses specific problems that can be solved using parameters characterizing the quality of the operator's activity during space flight, spacecraft development and operators' training.", "PMID": 966683}
{"id": "pubmed23n0032_6843", "title": "Rabbit mortality survey.", "content": "In a survey of mortality in 2224 rabbits of up to 12 weeks of age, drawn from 10 production units, neonatal mortality was important with 111 stillbirths and 175 deaths in the 1st week of life out of a total of 457 deaths in the whole survey. There was a low overall incidence of death from respiratory and enteric disease during the period of study.", "contents": "Rabbit mortality survey. In a survey of mortality in 2224 rabbits of up to 12 weeks of age, drawn from 10 production units, neonatal mortality was important with 111 stillbirths and 175 deaths in the 1st week of life out of a total of 457 deaths in the whole survey. There was a low overall incidence of death from respiratory and enteric disease during the period of study.", "PMID": 966692}
{"id": "pubmed23n0032_6844", "title": "Experimental induction of cervical lymphadenitis in guinea-pigs with group C streptococci.", "content": "Streptococcal lymphadenitis with macroscopic abscesses was induced in guinea-pigs when an isolate of Lancefield's group C streptococci of guinea-pig origin was sprayed orally. The disease was also produced in guinea-pigs when another isolate was injected sublingually but not when it was sprayed orally. Treatment with prednisolone did not increase the susceptibility to the latter isolant when sprayed orally. Abscesses could not be induced in the cervical lymph nodes of guinea-pigs exposed by injecting group E streptococci sublingually, although the organism was isolated from the cervical lymph nodes 2 days after inoculation. Neither could abscesses be induced by injecting these streptococci sublingually in guinea-pigs treated with prednisolone.", "contents": "Experimental induction of cervical lymphadenitis in guinea-pigs with group C streptococci. Streptococcal lymphadenitis with macroscopic abscesses was induced in guinea-pigs when an isolate of Lancefield's group C streptococci of guinea-pig origin was sprayed orally. The disease was also produced in guinea-pigs when another isolate was injected sublingually but not when it was sprayed orally. Treatment with prednisolone did not increase the susceptibility to the latter isolant when sprayed orally. Abscesses could not be induced in the cervical lymph nodes of guinea-pigs exposed by injecting group E streptococci sublingually, although the organism was isolated from the cervical lymph nodes 2 days after inoculation. Neither could abscesses be induced by injecting these streptococci sublingually in guinea-pigs treated with prednisolone.", "PMID": 966693}
{"id": "pubmed23n0032_6845", "title": "Seizure resistance in albino gerbils.", "content": "Albino gerbils (Meriones unguiculatus) and their agouti litter mates were tested once per week for 10-16 weeks for spontaneous epileptiform seizures when transferred to a novel environment. Only 16% of the albinos had seizures compared with 41% among the agoutis (P less than 0-05).", "contents": "Seizure resistance in albino gerbils. Albino gerbils (Meriones unguiculatus) and their agouti litter mates were tested once per week for 10-16 weeks for spontaneous epileptiform seizures when transferred to a novel environment. Only 16% of the albinos had seizures compared with 41% among the agoutis (P less than 0-05).", "PMID": 966694}
{"id": "pubmed23n0032_6846", "title": "The distribution of enzymes in the skin of the domestic pig.", "content": "On the basis of enzyme histochemical methods, the distribution and the activities of various enzymes in the skin of the domestic pig were investigated. The results are discussed in connection with the importance of the domestic pig as an experimental animal for dermatological research.", "contents": "The distribution of enzymes in the skin of the domestic pig. On the basis of enzyme histochemical methods, the distribution and the activities of various enzymes in the skin of the domestic pig were investigated. The results are discussed in connection with the importance of the domestic pig as an experimental animal for dermatological research.", "PMID": 966695}
{"id": "pubmed23n0032_6847", "title": "[Experiment with rats on a 22-day flight on the \"Kosmos-605\" biological satellite (objectives and methods)].", "content": "In 1974 a rat experiment was carried out onboard the Cosmos-605 biosatellite. Inflight Wistar rats were kept unrestrained in small cages. The cages were equipped with a feeder, water supply, light source and a ventilation device. The state of the animals was assessed with respect to their motor activity. The flight experiment was preceded by a number of preparatory runs and testinns that were completed with an end-to-end experiment in a biosatellite mockup. The flight experiment was paralleled by the ground-based synchroneous experiment which simulated almost entirely the flight profile. For each experiment rats were selected and trained during a month's observation. Postflight rats were exposed to clinical, physiological, morphological, cytochemical and biochemical investigations. Tissue examinations were performed on the 2nd-3rd day (20 rats) and 26-27th day (12 rats) after flight. Four rats were kept to study remote aftereffects.", "contents": "[Experiment with rats on a 22-day flight on the \"Kosmos-605\" biological satellite (objectives and methods)]. In 1974 a rat experiment was carried out onboard the Cosmos-605 biosatellite. Inflight Wistar rats were kept unrestrained in small cages. The cages were equipped with a feeder, water supply, light source and a ventilation device. The state of the animals was assessed with respect to their motor activity. The flight experiment was preceded by a number of preparatory runs and testinns that were completed with an end-to-end experiment in a biosatellite mockup. The flight experiment was paralleled by the ground-based synchroneous experiment which simulated almost entirely the flight profile. For each experiment rats were selected and trained during a month's observation. Postflight rats were exposed to clinical, physiological, morphological, cytochemical and biochemical investigations. Tissue examinations were performed on the 2nd-3rd day (20 rats) and 26-27th day (12 rats) after flight. Four rats were kept to study remote aftereffects.", "PMID": 966690}
{"id": "pubmed23n0032_6848", "title": "The effects of age and anaesthetic on the blood picture of the cuis, Galea musteloides.", "content": "Blood samples from 25 adult and 6 immature cuis were examined. There were slight differences in red cell parameters (RBC, Hb, PCV, MCV, MCH, & MCHC) between cuis and guinea-pigs. The total white cell count of cuis was much lower. The neutrophils of the cuis, as of the guinea-pig, had eosinophilic granules and were therefore termed pseudoeosinophils. Kurloff bodies were not present in mononuclear cells. There were more platelets in cuis blood compared with guinea-pig blood. The choice of anaesthetic did not influence any of the red cell parameters of the cuis, but may have affected the relative numbers of pseudoeosinophils, the platelet count and the percentage of reticulocytes. Immature animals had a lower erythrocyte count, a higher MCH and fewer platelets than adults.", "contents": "The effects of age and anaesthetic on the blood picture of the cuis, Galea musteloides. Blood samples from 25 adult and 6 immature cuis were examined. There were slight differences in red cell parameters (RBC, Hb, PCV, MCV, MCH, & MCHC) between cuis and guinea-pigs. The total white cell count of cuis was much lower. The neutrophils of the cuis, as of the guinea-pig, had eosinophilic granules and were therefore termed pseudoeosinophils. Kurloff bodies were not present in mononuclear cells. There were more platelets in cuis blood compared with guinea-pig blood. The choice of anaesthetic did not influence any of the red cell parameters of the cuis, but may have affected the relative numbers of pseudoeosinophils, the platelet count and the percentage of reticulocytes. Immature animals had a lower erythrocyte count, a higher MCH and fewer platelets than adults.", "PMID": 966696}
{"id": "pubmed23n0032_6849", "title": "An improved method for the production of experimental congestive heart failure in the guinea-pig.", "content": "The method involves supravalvular aortic constriction, and a simple, efficient, aortic constrictor has been developed which gives highly predictable results. Mortality due to causes directly related to the surgical procedure was low.", "contents": "An improved method for the production of experimental congestive heart failure in the guinea-pig. The method involves supravalvular aortic constriction, and a simple, efficient, aortic constrictor has been developed which gives highly predictable results. Mortality due to causes directly related to the surgical procedure was low.", "PMID": 966697}
{"id": "pubmed23n0032_6850", "title": "Measurement of digestible and metabolizable energy of diets for rats.", "content": "A balance method for the measurement of digestible and metabolizable energy in rats is described. Experimentally determined metabolizable energies of 2 diets for rats were closer to those values for metabolizable energy calculated from tabulated values for pigs rather than chickens. Some drawbacks in the use of tables of energy values to predict the energy of diets are discussed.", "contents": "Measurement of digestible and metabolizable energy of diets for rats. A balance method for the measurement of digestible and metabolizable energy in rats is described. Experimentally determined metabolizable energies of 2 diets for rats were closer to those values for metabolizable energy calculated from tabulated values for pigs rather than chickens. Some drawbacks in the use of tables of energy values to predict the energy of diets are discussed.", "PMID": 966698}
{"id": "pubmed23n0032_6851", "title": "Effects of stress due to deprivation and transport in different genotypes of house mouse.", "content": "The importance of various stress factors involved in boxing and transit of wild and laboratory mice on a 28 hour journey was studied. Transference from laboratory cage to transit box alone caused weight loss; under the best conditions the laboratory mice lost 5% of their initial weight and wild ones 8%. Deprivation of food resulted in absolute loss of weight; from this death ensued when 20% of initial weight was lost: the smaller wild mice died sooner than the larger laboratory ones. Water deprivation resulted in retarding recovery of weight lost; wild mice took longer to recover than laboratory ones. It also caused weight loss and, in conjunction with deprivation of food, poor condition and death. Transit itself affected percentage weight loss and wheat consumption; wild mice were affected differently from laboratory mice in both respects. Genotype is seen to be important not only in controlling initial weight and activity level, but also--and independently of weight--the total food requirement. In the light of these findings current literature giving guidance on shipment of small mammals is shown to be inadequate, and certain recommendations are given.", "contents": "Effects of stress due to deprivation and transport in different genotypes of house mouse. The importance of various stress factors involved in boxing and transit of wild and laboratory mice on a 28 hour journey was studied. Transference from laboratory cage to transit box alone caused weight loss; under the best conditions the laboratory mice lost 5% of their initial weight and wild ones 8%. Deprivation of food resulted in absolute loss of weight; from this death ensued when 20% of initial weight was lost: the smaller wild mice died sooner than the larger laboratory ones. Water deprivation resulted in retarding recovery of weight lost; wild mice took longer to recover than laboratory ones. It also caused weight loss and, in conjunction with deprivation of food, poor condition and death. Transit itself affected percentage weight loss and wheat consumption; wild mice were affected differently from laboratory mice in both respects. Genotype is seen to be important not only in controlling initial weight and activity level, but also--and independently of weight--the total food requirement. In the light of these findings current literature giving guidance on shipment of small mammals is shown to be inadequate, and certain recommendations are given.", "PMID": 966699}
{"id": "pubmed23n0032_6852", "title": "Effect of age and sex on histamine-induced gastric secretion in laboratory rats.", "content": "The effect of age and sex on histamine-stimulated 24-hr gastric secretion was assessed in the rat. Secretion tests were done in rats of both sexes at 3, 6, 9, 12, 18, and 24 mo of age. The highest 24-hr volume and acid output of gastric secretion was found in both sexes in the 9-mo age group. When related to body weight the highest 24-hr gastric secretion and acid output was found in 6-mo-old males and in 9-mo-old females. This observation may have practical value when the rat is selected as an experimental tool for the study of gastric secretory function.", "contents": "Effect of age and sex on histamine-induced gastric secretion in laboratory rats. The effect of age and sex on histamine-stimulated 24-hr gastric secretion was assessed in the rat. Secretion tests were done in rats of both sexes at 3, 6, 9, 12, 18, and 24 mo of age. The highest 24-hr volume and acid output of gastric secretion was found in both sexes in the 9-mo age group. When related to body weight the highest 24-hr gastric secretion and acid output was found in 6-mo-old males and in 9-mo-old females. This observation may have practical value when the rat is selected as an experimental tool for the study of gastric secretory function.", "PMID": 966701}
{"id": "pubmed23n0032_6853", "title": "Reproductive performance and fertility testing in strain 13 and Hartley guinea pigs.", "content": "A study to test the effects of certain experimental manipulation on the reproductive capacity of male guinea pigs required verifying the fertility of the male guinea pigs before and after manipulation. Methods of testing fertility were evaluated, and normal reproductive data from preexperimental and control groups were tabulated and analyzed. No data from the actual experiments were included. Virgin and proven fertile males were mated with 1 (1:1) or 2 (2:1) virgin or proven fertile females. Inbred (13/N Umm) and conventional (Mfi:CFDH-ML (DH) ) guinea pigs were used. Ninety-five percent of both groups of males were fertile. Eighty-four percent of both groups of females were fertile. Male guinea pigs previously proven fertile had the same subsequent fertility rate as virgin males. Over one-third of the conceptions did not take place during the first estrus cycle (16 da) during which the males and females were mated. Strain 13 and Hartley females had litters of approximately the same size (3.1 vs 3.0), but the neonatal mortality was statistically lower (P less than 0.001) in the Hartley stock (9.3%) than in the Strain 13 guinea pigs (28.4%).", "contents": "Reproductive performance and fertility testing in strain 13 and Hartley guinea pigs. A study to test the effects of certain experimental manipulation on the reproductive capacity of male guinea pigs required verifying the fertility of the male guinea pigs before and after manipulation. Methods of testing fertility were evaluated, and normal reproductive data from preexperimental and control groups were tabulated and analyzed. No data from the actual experiments were included. Virgin and proven fertile males were mated with 1 (1:1) or 2 (2:1) virgin or proven fertile females. Inbred (13/N Umm) and conventional (Mfi:CFDH-ML (DH) ) guinea pigs were used. Ninety-five percent of both groups of males were fertile. Eighty-four percent of both groups of females were fertile. Male guinea pigs previously proven fertile had the same subsequent fertility rate as virgin males. Over one-third of the conceptions did not take place during the first estrus cycle (16 da) during which the males and females were mated. Strain 13 and Hartley females had litters of approximately the same size (3.1 vs 3.0), but the neonatal mortality was statistically lower (P less than 0.001) in the Hartley stock (9.3%) than in the Strain 13 guinea pigs (28.4%).", "PMID": 966702}
{"id": "pubmed23n0032_6854", "title": "Record-keeping for a colony of 15,000 mice without use of a computer.", "content": "An economical and easy to learn method of record-keeping was developed which utilizes standard office supply 3 5 cards for cage cards and central office records. The method developed has been used to maintain all records of a mouse colony of 18 inbred strains and substrains for 9 yr. With this system, detailed pedigrees, postmortem data, tumor incidence, and average age are available in minutes.", "contents": "Record-keeping for a colony of 15,000 mice without use of a computer. An economical and easy to learn method of record-keeping was developed which utilizes standard office supply 3 5 cards for cage cards and central office records. The method developed has been used to maintain all records of a mouse colony of 18 inbred strains and substrains for 9 yr. With this system, detailed pedigrees, postmortem data, tumor incidence, and average age are available in minutes.", "PMID": 966703}
{"id": "pubmed23n0032_6855", "title": "Measurement of intestinal absorption in mice by a double-label radioisotope perfusion technic.", "content": "The use of the standard chemical technic for measuring intestinal glucose absorption was compared with a new double-label radioisotope technic. Glucose absorption and water flux in the mouse small intestine were measured by both methods. The results indicated that the 2 approaches yield almost identical values. The radioisotope technic utilized [14C] polyethylene glycol to measure water flux and [3H] glucose. The technic was found to be reliable, rapid, and applicable to small samples and other substrates. It is particularly suited to absorption studies in small animals such as the mouse, where sample size is limited. The study also showed that the polyethylene glycol recovery rate from the mouse intestine was 97.8%, indicating that it is a valid absorption marker in this species.", "contents": "Measurement of intestinal absorption in mice by a double-label radioisotope perfusion technic. The use of the standard chemical technic for measuring intestinal glucose absorption was compared with a new double-label radioisotope technic. Glucose absorption and water flux in the mouse small intestine were measured by both methods. The results indicated that the 2 approaches yield almost identical values. The radioisotope technic utilized [14C] polyethylene glycol to measure water flux and [3H] glucose. The technic was found to be reliable, rapid, and applicable to small samples and other substrates. It is particularly suited to absorption studies in small animals such as the mouse, where sample size is limited. The study also showed that the polyethylene glycol recovery rate from the mouse intestine was 97.8%, indicating that it is a valid absorption marker in this species.", "PMID": 966704}
{"id": "pubmed23n0032_6856", "title": "Intracisternal virus-like particles in two guinea pig mammary adenocarcinomas.", "content": "Virus-like particles were found in spontaneously arising mammary adenocarcinomas from 2 male guinea pigs. The type-B particles were 100 nm in diameter and budded from the apex of stalks into cisternae. Examination of normal and neoplastic tissues from other guinea pigs failed to reveal similar particles. A review of the literature revealed that of the 16 known cases of mammary adenocarcinomas in guinea pigs, 5 have occurred in male animals. The apparent propensity for spontaneous mammary adenocarcinomas in males of this species is discussed in relation to the possible etiologic agents responsible for them.", "contents": "Intracisternal virus-like particles in two guinea pig mammary adenocarcinomas. Virus-like particles were found in spontaneously arising mammary adenocarcinomas from 2 male guinea pigs. The type-B particles were 100 nm in diameter and budded from the apex of stalks into cisternae. Examination of normal and neoplastic tissues from other guinea pigs failed to reveal similar particles. A review of the literature revealed that of the 16 known cases of mammary adenocarcinomas in guinea pigs, 5 have occurred in male animals. The apparent propensity for spontaneous mammary adenocarcinomas in males of this species is discussed in relation to the possible etiologic agents responsible for them.", "PMID": 966705}
{"id": "pubmed23n0032_6857", "title": "A restraining device and oral dosing technic for the chinchilla (Chinchilla laniger).", "content": "A device, based on the principle of a cattle squeeze chute, was designed for restraining a chinchilla; and a cannula-tipped syringe was designed for introducing a solution into the animal's throat. The restraining device allows 1 person to conduct routine clinical procedures while the cannula-tipped syringe facilitates oral administration of liquids.", "contents": "A restraining device and oral dosing technic for the chinchilla (Chinchilla laniger). A device, based on the principle of a cattle squeeze chute, was designed for restraining a chinchilla; and a cannula-tipped syringe was designed for introducing a solution into the animal's throat. The restraining device allows 1 person to conduct routine clinical procedures while the cannula-tipped syringe facilitates oral administration of liquids.", "PMID": 966706}
{"id": "pubmed23n0032_6858", "title": "Blood and serum chemistry values of gnotobiotic beagles.", "content": "Several blood and serum chemistry values were serially determined over a 2-yr period on 17 purebred beagle dogs from the same closed, inbred colony. Seven of these dogs were germfree, and 10 had a conventional bacterial flora. Seven of those with a conventional flora were confined in germfree-type isolators; 3 were housed in a routine manner in a conventional animal facility. The germfree dogs were found to have significantly lower red blood cell, white blood cell, neutrophil, and band cell counts lower levels of alpha-2 and gamma globulins, and higher amounts of cholesterol and alkaline phosphatase than the 2 sets of conventional dogs. In most other respects, the blood and serum chemistry values of the germfree dogs were similar to those of the conventional dogs and to previously reported values for normal beagles.", "contents": "Blood and serum chemistry values of gnotobiotic beagles. Several blood and serum chemistry values were serially determined over a 2-yr period on 17 purebred beagle dogs from the same closed, inbred colony. Seven of these dogs were germfree, and 10 had a conventional bacterial flora. Seven of those with a conventional flora were confined in germfree-type isolators; 3 were housed in a routine manner in a conventional animal facility. The germfree dogs were found to have significantly lower red blood cell, white blood cell, neutrophil, and band cell counts lower levels of alpha-2 and gamma globulins, and higher amounts of cholesterol and alkaline phosphatase than the 2 sets of conventional dogs. In most other respects, the blood and serum chemistry values of the germfree dogs were similar to those of the conventional dogs and to previously reported values for normal beagles.", "PMID": 966707}
{"id": "pubmed23n0032_6859", "title": "Effect of deworming medication on the microbial flora of the upper gastrointestinal tract of dogs.", "content": "Control cultures were taken at laporatomy of the stomach or stomach remnant, jejunum, and ileum of 26 adult dogs, 8 of which had had gastric surgery 1 yr earlier. Two wk after the control cultures were taken, 17 of the 26 dogs, including all 8 which had had prior surgery, were treated with anthelmintics, niclosamide, dichlorophene, methylbenzene, and arecoline hydrobromide. Two wk after the anthelmintic treatment, or 4 wk after the control cultures were taken, the 26 dogs were recultured. Samples of microbial flora were obtained by direct needle aspiration employing anaerobic precautions. These samples were processed both quantitatively and qualitatively using both anaerobic and aerobic technics. The specific sites cultured were the lower stomach or stomach remnant, proximal jejunum 15 cm distal to the ligament of Treitz, and distal ileum 45 cm proximal to the ileocecal valve. Bacteria isolated were predominately facultative aerobes. No fungi were isolated. Cultivation of spirochetes was not attempted. The results showed that there was no significant qualitative or quantitative alteration of microbial flora caused by the anthelmintic treatment.", "contents": "Effect of deworming medication on the microbial flora of the upper gastrointestinal tract of dogs. Control cultures were taken at laporatomy of the stomach or stomach remnant, jejunum, and ileum of 26 adult dogs, 8 of which had had gastric surgery 1 yr earlier. Two wk after the control cultures were taken, 17 of the 26 dogs, including all 8 which had had prior surgery, were treated with anthelmintics, niclosamide, dichlorophene, methylbenzene, and arecoline hydrobromide. Two wk after the anthelmintic treatment, or 4 wk after the control cultures were taken, the 26 dogs were recultured. Samples of microbial flora were obtained by direct needle aspiration employing anaerobic precautions. These samples were processed both quantitatively and qualitatively using both anaerobic and aerobic technics. The specific sites cultured were the lower stomach or stomach remnant, proximal jejunum 15 cm distal to the ligament of Treitz, and distal ileum 45 cm proximal to the ileocecal valve. Bacteria isolated were predominately facultative aerobes. No fungi were isolated. Cultivation of spirochetes was not attempted. The results showed that there was no significant qualitative or quantitative alteration of microbial flora caused by the anthelmintic treatment.", "PMID": 966708}
{"id": "pubmed23n0032_6860", "title": "A system for inducing hyperthermia in sheep.", "content": "A system that produces hyperthermia in pregnant sheep in a consistent and reproducible manner was designed. The experimental approach was through control of ambient temperature and relative humidity in a closed chamber; the sheep was unable to lose its metabolic heat in an environment of 42 degrees C and 75% relative humidity. This system resulted in a steady and progressive temperature rise of 2.5 degrees C in the maternal abdominal aorta in 120 +/- 17 min.", "contents": "A system for inducing hyperthermia in sheep. A system that produces hyperthermia in pregnant sheep in a consistent and reproducible manner was designed. The experimental approach was through control of ambient temperature and relative humidity in a closed chamber; the sheep was unable to lose its metabolic heat in an environment of 42 degrees C and 75% relative humidity. This system resulted in a steady and progressive temperature rise of 2.5 degrees C in the maternal abdominal aorta in 120 +/- 17 min.", "PMID": 966709}
{"id": "pubmed23n0032_6861", "title": "Koni, kona, kava. orange-beer culture of the Cook islands.", "content": "The orange-beer culture of the Cook Islands is described. Island drinking customs were found to have cultlike forms, provide for social integration, and control drunken aggression.", "contents": "Koni, kona, kava. orange-beer culture of the Cook islands. The orange-beer culture of the Cook Islands is described. Island drinking customs were found to have cultlike forms, provide for social integration, and control drunken aggression.", "PMID": 966767}
{"id": "pubmed23n0032_6862", "title": "Cerebral blood flow and its regional distribution in alcoholism and in Korsakoff's psychosis.", "content": "The regional cerebral blood flow in alcoholics decreased with age. This reduction may be associated with the length of excessive drinking.", "contents": "Cerebral blood flow and its regional distribution in alcoholism and in Korsakoff's psychosis. The regional cerebral blood flow in alcoholics decreased with age. This reduction may be associated with the length of excessive drinking.", "PMID": 966768}
{"id": "pubmed23n0032_6863", "title": "Effects of rate of drinking on human performance.", "content": "Performance on tests of motor control and of central processing of information was more impaired in rapid drinkers than in slower drinkers.", "contents": "Effects of rate of drinking on human performance. Performance on tests of motor control and of central processing of information was more impaired in rapid drinkers than in slower drinkers.", "PMID": 966769}
{"id": "pubmed23n0032_6864", "title": "Alcohol-induced taste aversion in golden hamsters.", "content": "Multiple injections of alcohol were effective in producing substantial aversions to saccharin-flavored water in golden hamsters. A dose of 1.76 g of alcohol per kg produced a long-lasting aversion, whereas aversion produced by injections of 1.17 g per kg extinguished rapidly.", "contents": "Alcohol-induced taste aversion in golden hamsters. Multiple injections of alcohol were effective in producing substantial aversions to saccharin-flavored water in golden hamsters. A dose of 1.76 g of alcohol per kg produced a long-lasting aversion, whereas aversion produced by injections of 1.17 g per kg extinguished rapidly.", "PMID": 966770}
{"id": "pubmed23n0032_6865", "title": "Alcoholics view group therapy. Process and goals.", "content": "Alcoholics ranked certain elements of group therapy as more or less helpful, depending on the stage of therapy they were in.", "contents": "Alcoholics view group therapy. Process and goals. Alcoholics ranked certain elements of group therapy as more or less helpful, depending on the stage of therapy they were in.", "PMID": 966771}
{"id": "pubmed23n0032_6866", "title": "Neighborhood reactions to a community-based treatment center for alcoholics.", "content": "Survey data from residents of a neighborhood located near a controversial community-based treatment center for alcoholics were compared with data from a similar neighborhood with no treatment facility. The results suggest that neighborhood opposition to such facilities may not be as intense as is commonly thought.", "contents": "Neighborhood reactions to a community-based treatment center for alcoholics. Survey data from residents of a neighborhood located near a controversial community-based treatment center for alcoholics were compared with data from a similar neighborhood with no treatment facility. The results suggest that neighborhood opposition to such facilities may not be as intense as is commonly thought.", "PMID": 966772}
{"id": "pubmed23n0032_6867", "title": "Reconciling unidimensional and multidimensional models of patterns of drug use.", "content": "Guttman-scale analyses and factor analyses seem to produce contradictory results in analyzing patterns of multiple drug use. Guttman-scale analyses usually show that drug use fits a unidimensional and cumulative model of involvement; factor analyses reject the unidimensional model. The two methods, however, can be reconciled. Guttman scaling is appropriate for discerning patterns of drug involvement, factor analysis for operationalizing degree of involvement within a specific pattern of use.", "contents": "Reconciling unidimensional and multidimensional models of patterns of drug use. Guttman-scale analyses and factor analyses seem to produce contradictory results in analyzing patterns of multiple drug use. Guttman-scale analyses usually show that drug use fits a unidimensional and cumulative model of involvement; factor analyses reject the unidimensional model. The two methods, however, can be reconciled. Guttman scaling is appropriate for discerning patterns of drug involvement, factor analysis for operationalizing degree of involvement within a specific pattern of use.", "PMID": 966773}
{"id": "pubmed23n0032_6868", "title": "Women arrested for drunken driving in Boston. Social characteristics and circumstances of arrest.", "content": "The social characteristics and circumstances of arrest of 73 women arrested for driving while intoxicated and referred to the Boston Alcohol Safety Action Project were examined. Driving while intoxicated was a necessary but not sufficient condition to accoutn for the arrests.", "contents": "Women arrested for drunken driving in Boston. Social characteristics and circumstances of arrest. The social characteristics and circumstances of arrest of 73 women arrested for driving while intoxicated and referred to the Boston Alcohol Safety Action Project were examined. Driving while intoxicated was a necessary but not sufficient condition to accoutn for the arrests.", "PMID": 966774}
{"id": "pubmed23n0032_6869", "title": "Effect of a negative stereotype on nurses' attitudes toward an alcoholic patient.", "content": "Nurses' impressions of a hypothetical patient, labeled an alcoholic, were strongly influenced by their negative stereotypic views of alcoholics. Nurses responded more favorably to the same \"patient\" when he was not labeled an alcoholic even though the behavior of the patient was held constant.", "contents": "Effect of a negative stereotype on nurses' attitudes toward an alcoholic patient. Nurses' impressions of a hypothetical patient, labeled an alcoholic, were strongly influenced by their negative stereotypic views of alcoholics. Nurses responded more favorably to the same \"patient\" when he was not labeled an alcoholic even though the behavior of the patient was held constant.", "PMID": 966775}
{"id": "pubmed23n0032_6870", "title": "Missouri statewide alcoholism treatment survey.", "content": "Respondents to a statewide survey of alcoholism treatment services throught that prevention should be given prime priority consideration.", "contents": "Missouri statewide alcoholism treatment survey. Respondents to a statewide survey of alcoholism treatment services throught that prevention should be given prime priority consideration.", "PMID": 966776}
{"id": "pubmed23n0032_6871", "title": "A.A. and group therapy for alcoholics. An application of the World Hypotheses Scale.", "content": "The results of a study suggest that alcoholics with a preference for formistic thinking (as defined by Pepper's World Hypotheses) are more likely to benefit from participation in Alcholics Anonymous than from group therapy.", "contents": "A.A. and group therapy for alcoholics. An application of the World Hypotheses Scale. The results of a study suggest that alcoholics with a preference for formistic thinking (as defined by Pepper's World Hypotheses) are more likely to benefit from participation in Alcholics Anonymous than from group therapy.", "PMID": 966777}
{"id": "pubmed23n0032_6872", "title": "Personality traits and moods of alcoholics and heroin addicts.", "content": "No differences in moods were found between alcoholics and drug addicts, but the alcoholics were significantly more neurotic than the addicts.", "contents": "Personality traits and moods of alcoholics and heroin addicts. No differences in moods were found between alcoholics and drug addicts, but the alcoholics were significantly more neurotic than the addicts.", "PMID": 966778}
{"id": "pubmed23n0032_6873", "title": "Evaluation of the randomized response technique in a drinking survey.", "content": "A randomized response questionnaire elicited more positive responses from high-school students on an alcohol-related question than did  a self-report questionnaire.", "contents": "Evaluation of the randomized response technique in a drinking survey. A randomized response questionnaire elicited more positive responses from high-school students on an alcohol-related question than did  a self-report questionnaire.", "PMID": 966779}
{"id": "pubmed23n0032_6874", "title": "Control orientation in women alcoholics.", "content": "Women alcoholics had higher scores than women social drinkers (indicating greater external control orientation) on both the Internal-External Locus of Control Scale and a Locus of Drinking Control Scale.", "contents": "Control orientation in women alcoholics. Women alcoholics had higher scores than women social drinkers (indicating greater external control orientation) on both the Internal-External Locus of Control Scale and a Locus of Drinking Control Scale.", "PMID": 966780}
{"id": "pubmed23n0032_6875", "title": "Psychomotor and intellectual development after deep hypothermia and circulatory arrest in early infancy.", "content": "Somatic, psychomotor, and intellectual development were studied in 11 patients who had total correction of congenital cardiac malformation in early infancy by the method of surface-induced deep hypothermia and circulatory arrest up to 43 minutes. Time between operation and re-evaluation ranged from 3 to 11 years (average 7 years). Somatic development was normal in all children. Psychomotor and intellectual behavior, measured by a battery of specially adapted test methods, failed to show a statistically significant difference when compared between the study group, a matched control group, and the normal population. Normal scores in psychomotor tests and normal intelligence quotients (I.Q.) were present in 8 patients; mental retardation was found in 2 patients: and unstable behavior, evident from different tests, was present in one. Except for the last case, in none of the patients was the method was found to be the primary cause of the abnormal behavior. Deep hypothermia and circulatory arrest in early infancy does not appear to have a negative influence on somatic, psychomotor, or intellectual development.", "contents": "Psychomotor and intellectual development after deep hypothermia and circulatory arrest in early infancy. Somatic, psychomotor, and intellectual development were studied in 11 patients who had total correction of congenital cardiac malformation in early infancy by the method of surface-induced deep hypothermia and circulatory arrest up to 43 minutes. Time between operation and re-evaluation ranged from 3 to 11 years (average 7 years). Somatic development was normal in all children. Psychomotor and intellectual behavior, measured by a battery of specially adapted test methods, failed to show a statistically significant difference when compared between the study group, a matched control group, and the normal population. Normal scores in psychomotor tests and normal intelligence quotients (I.Q.) were present in 8 patients; mental retardation was found in 2 patients: and unstable behavior, evident from different tests, was present in one. Except for the last case, in none of the patients was the method was found to be the primary cause of the abnormal behavior. Deep hypothermia and circulatory arrest in early infancy does not appear to have a negative influence on somatic, psychomotor, or intellectual development.", "PMID": 966781}
{"id": "pubmed23n0032_6876", "title": "Acute clinical hypocalcemic myocardial depression during rapid blood transfusion and postoperative hemodialysis: a preventable complication.", "content": "Despite experimental evidence that myocardial depression resulting from rapid transfusion of ACD blood (citrate binds ionic calcium) is avoidable by simultaneous calcium administration, most hypovolemic patients receive calcium either after transfusion or not at all. Similar iatrogenic hypocalcemic myocardial depression occurs in normovolemic patients with known myocardial damage who are dialyzed for acute uremia when ACD blood prime is used at high initial flow rates (350 c.c. per minute) and when dialysis is performed against low calcium dialysate (2.5 mEq. per liter or less). This study tests the hypotheses that (1) rapid transfusion of as little as one unit of CPD blood causes a significant reduction in ionized calcium, (2) the depressive effect of CPD blood is significant and similar to that of ACD blood, (3) rapid blood transfusion (ACD or CPD) is safe if calcium is given simultaneously, (4) addition of calcium to the extracorporeal heparinized blood prime used in dialysis prevents initial depression, and (5) hemodynamic instability during dialysis is prevented when the dialysate is normocalcemic. From the results of our study, we made the following conclusions: (1) Ionized calcium is reduced significantly by rapid transfusion of CPD blood; (2) acute myocardial depression noted with CPD blood is similar to that previously observed with ACD blood and is prevented during transfusion of either type of blood by simultaneous calcium administration; and (3) hemodialysis in patients who have had cardiac surgery is safe if calcium is added to blood prime and dialysate is made normocalcemic.", "contents": "Acute clinical hypocalcemic myocardial depression during rapid blood transfusion and postoperative hemodialysis: a preventable complication. Despite experimental evidence that myocardial depression resulting from rapid transfusion of ACD blood (citrate binds ionic calcium) is avoidable by simultaneous calcium administration, most hypovolemic patients receive calcium either after transfusion or not at all. Similar iatrogenic hypocalcemic myocardial depression occurs in normovolemic patients with known myocardial damage who are dialyzed for acute uremia when ACD blood prime is used at high initial flow rates (350 c.c. per minute) and when dialysis is performed against low calcium dialysate (2.5 mEq. per liter or less). This study tests the hypotheses that (1) rapid transfusion of as little as one unit of CPD blood causes a significant reduction in ionized calcium, (2) the depressive effect of CPD blood is significant and similar to that of ACD blood, (3) rapid blood transfusion (ACD or CPD) is safe if calcium is given simultaneously, (4) addition of calcium to the extracorporeal heparinized blood prime used in dialysis prevents initial depression, and (5) hemodynamic instability during dialysis is prevented when the dialysate is normocalcemic. From the results of our study, we made the following conclusions: (1) Ionized calcium is reduced significantly by rapid transfusion of CPD blood; (2) acute myocardial depression noted with CPD blood is similar to that previously observed with ACD blood and is prevented during transfusion of either type of blood by simultaneous calcium administration; and (3) hemodialysis in patients who have had cardiac surgery is safe if calcium is added to blood prime and dialysate is made normocalcemic.", "PMID": 966782}
{"id": "pubmed23n0032_6877", "title": "Preoperative assessment of esophageal pathology.", "content": "Panmural esophagitis results in esophageal thickening and shortening and prevents adequate reduction of a hernia. Twenty patients with panmural esophagitis, treated by Belsey repair, have been followed up for more than 5 years; 9 of them remain asymptomatic and 11 have symptomatic reflux, 7 of whom have required further surgery. Belsey also has reported a 45 per cent recurrence rate in patients with this type of disease. Preoperative recognition of panmural esophagitis allows a planned surgical approach and the use of a surgical technique designed for the management of an irreducible hernia. The ability to predict these changes was studied in 124 patients, who were evaluated by history, radiology, endoscopy, and manometry prior to transthoracic hernia repair. The esophagus was inspected at operation to determine the presence of panmural changes. History was of no value in assessment. Radiologically, a large and irreducible hernia was associated with panmural changes, but these changes also occurred in the absence of ulceration. Manometric studies allowed accurate prediction of mural changes. Over 90 per cent of patients with panmural esophagitis have more than 40 per cent disordered motor activity (DMA) in the distal part of the esophagus, and 75 per cent of such patients have more than 60 per cent DMA. Combining these investigative data allowed the accurate prediction of panmural changes in 90 per cent of the 124 patients.", "contents": "Preoperative assessment of esophageal pathology. Panmural esophagitis results in esophageal thickening and shortening and prevents adequate reduction of a hernia. Twenty patients with panmural esophagitis, treated by Belsey repair, have been followed up for more than 5 years; 9 of them remain asymptomatic and 11 have symptomatic reflux, 7 of whom have required further surgery. Belsey also has reported a 45 per cent recurrence rate in patients with this type of disease. Preoperative recognition of panmural esophagitis allows a planned surgical approach and the use of a surgical technique designed for the management of an irreducible hernia. The ability to predict these changes was studied in 124 patients, who were evaluated by history, radiology, endoscopy, and manometry prior to transthoracic hernia repair. The esophagus was inspected at operation to determine the presence of panmural changes. History was of no value in assessment. Radiologically, a large and irreducible hernia was associated with panmural changes, but these changes also occurred in the absence of ulceration. Manometric studies allowed accurate prediction of mural changes. Over 90 per cent of patients with panmural esophagitis have more than 40 per cent disordered motor activity (DMA) in the distal part of the esophagus, and 75 per cent of such patients have more than 60 per cent DMA. Combining these investigative data allowed the accurate prediction of panmural changes in 90 per cent of the 124 patients.", "PMID": 966783}
{"id": "pubmed23n0032_6878", "title": "Management of acid-peptic esophageal strictures.", "content": "Eighty-nine patients with benign acid-peptic strictures of the esophagus were evaluated. Of these, 56 patients were treated by dilatation and medical therapy, whereas 33 underwent operative therapy because medical therapy failed. Three patients, all psychotic and having post-emetic strictures, required resection of the stricture and a colon interposition. Twenty-nine patients underwent a Belsey or Nissen fundoplication combined with esophageal dilatation. Fourteen of 29 had an associated vagotomy and 15 did not. Two patients of this 29 had a poor result, one of whom had a post-emetic stricture. Although the patients having a concurrent vagotomy and pyloroplasty had the more severe disease,there was no significant difference between the results in the two groups. It is concluded that most patients with such esophageal strictures may be adequately treated without resecting the stricture and that the routine addition of a vagotomy and drainage procedure is probably not indicated.", "contents": "Management of acid-peptic esophageal strictures. Eighty-nine patients with benign acid-peptic strictures of the esophagus were evaluated. Of these, 56 patients were treated by dilatation and medical therapy, whereas 33 underwent operative therapy because medical therapy failed. Three patients, all psychotic and having post-emetic strictures, required resection of the stricture and a colon interposition. Twenty-nine patients underwent a Belsey or Nissen fundoplication combined with esophageal dilatation. Fourteen of 29 had an associated vagotomy and 15 did not. Two patients of this 29 had a poor result, one of whom had a post-emetic stricture. Although the patients having a concurrent vagotomy and pyloroplasty had the more severe disease,there was no significant difference between the results in the two groups. It is concluded that most patients with such esophageal strictures may be adequately treated without resecting the stricture and that the routine addition of a vagotomy and drainage procedure is probably not indicated.", "PMID": 966784}
{"id": "pubmed23n0032_6879", "title": "Prosthetic reconstruction of the trachea and carina.", "content": "Twenty-six patients with tracheal stenosis owing to benign stricture and malignancy have had airway reconstruction with a molded silicone rubber prosthesis. In 8 patients the distal trachea and carina were replaced with a bifurcated graft. One individual with respiratory insufficiency could not be weaned from the respirator and died in 3 weeks. Two other patients with malignancy died 15 and 18 months following operation from disseminated cancer. The remainder are living from 1 to 5 years postoperatively. Eighteen individuals have had the trachea reconstructed with a straight graft of silicone rubber. In 5 patients with long strictures the prosthesis was invaginated into the upper and lower ends of the trachea. Thirteen individuals had circumferential resection of the airway with end-to-end anastomosis of the graft to the trachea. There was one early death from erosion of the innominate artery and 5 late deaths unrelated to the prosthesis.", "contents": "Prosthetic reconstruction of the trachea and carina. Twenty-six patients with tracheal stenosis owing to benign stricture and malignancy have had airway reconstruction with a molded silicone rubber prosthesis. In 8 patients the distal trachea and carina were replaced with a bifurcated graft. One individual with respiratory insufficiency could not be weaned from the respirator and died in 3 weeks. Two other patients with malignancy died 15 and 18 months following operation from disseminated cancer. The remainder are living from 1 to 5 years postoperatively. Eighteen individuals have had the trachea reconstructed with a straight graft of silicone rubber. In 5 patients with long strictures the prosthesis was invaginated into the upper and lower ends of the trachea. Thirteen individuals had circumferential resection of the airway with end-to-end anastomosis of the graft to the trachea. There was one early death from erosion of the innominate artery and 5 late deaths unrelated to the prosthesis.", "PMID": 966785}
{"id": "pubmed23n0032_6880", "title": "A hermetically sealed mercury cell pacemaker: advantages and early clinical results.", "content": "Despite the known advantages of hermetic encapsulation of implanted pacemakers, only a few conventionally powered models are totally hermetically sealed, and most of them are not yet clinically available. This report describes the results with a mercury cell unit which has been in clinical use since 1970 and has been totally hermetically sealed since 1974. The lowered premature failure rate with the newer model substantiates the theoretical advantages of hermetic encapsulation. The rate behavior of the pulse generator, upon which our telephone follow-up system is based, is essentially the same for both models.", "contents": "A hermetically sealed mercury cell pacemaker: advantages and early clinical results. Despite the known advantages of hermetic encapsulation of implanted pacemakers, only a few conventionally powered models are totally hermetically sealed, and most of them are not yet clinically available. This report describes the results with a mercury cell unit which has been in clinical use since 1970 and has been totally hermetically sealed since 1974. The lowered premature failure rate with the newer model substantiates the theoretical advantages of hermetic encapsulation. The rate behavior of the pulse generator, upon which our telephone follow-up system is based, is essentially the same for both models.", "PMID": 966786}
{"id": "pubmed23n0032_6881", "title": "Permanent pervenous atrial sensing and pacing with a new J-shaped lead.", "content": "We have developed a new J-shaped lead for atrial pacing. The lead has silicone rubber tines which become entangled in the trabeculae and thereby anchor the lead firmly in the left atrial appendage. Later, scar tissue forms around the tines, rather than around the electrode, so that there is no problem with either lead displacement or loss of sensing.", "contents": "Permanent pervenous atrial sensing and pacing with a new J-shaped lead. We have developed a new J-shaped lead for atrial pacing. The lead has silicone rubber tines which become entangled in the trabeculae and thereby anchor the lead firmly in the left atrial appendage. Later, scar tissue forms around the tines, rather than around the electrode, so that there is no problem with either lead displacement or loss of sensing.", "PMID": 966787}
{"id": "pubmed23n0032_6882", "title": "Aberrant left pulmonary artery presenting as a right paratracheal mass in an adult.", "content": "Aberrant left pulmonary artery, or pulmonary sling, is a rare variation of the pulmonary vasculature, with only one previous report of the condition being recognized in an adult. In this report a second case recognized de novo in an adult is presented. Both of these adults were evaluated because of right paratracheal masses which required differentiation from pulmonary malignancies. The barium swallow shows a characteristic indentation of the anterior esophageal wall and the lateral tomogram demonstrates absence of the left pulmonary artery from its normal position in the left hilum. The pulmonary arteriogram is diagnostic and allows avoidance of more invasive diagnostic measures.", "contents": "Aberrant left pulmonary artery presenting as a right paratracheal mass in an adult. Aberrant left pulmonary artery, or pulmonary sling, is a rare variation of the pulmonary vasculature, with only one previous report of the condition being recognized in an adult. In this report a second case recognized de novo in an adult is presented. Both of these adults were evaluated because of right paratracheal masses which required differentiation from pulmonary malignancies. The barium swallow shows a characteristic indentation of the anterior esophageal wall and the lateral tomogram demonstrates absence of the left pulmonary artery from its normal position in the left hilum. The pulmonary arteriogram is diagnostic and allows avoidance of more invasive diagnostic measures.", "PMID": 966788}
{"id": "pubmed23n0032_6883", "title": "Chondrosarcoma simulating malignant atrial myxoma.", "content": "A 69-year-old patient underwent removal of a left atrial tumor which was diagnosed clinically and pathologically as atrial myxoma. Fourteen, 18 and 22 months later, distant metastases were discovered. Two of the metastases were removed entirely and one partially. The metastatic tumors were also thought to be of myxomatous origin by light and electron microscopy. Only at autopsy was it demonstrated that the patient had a primary chondrosarcoma of the pelvis giving rise to widespread myxoid metastases to the heart and other sites.", "contents": "Chondrosarcoma simulating malignant atrial myxoma. A 69-year-old patient underwent removal of a left atrial tumor which was diagnosed clinically and pathologically as atrial myxoma. Fourteen, 18 and 22 months later, distant metastases were discovered. Two of the metastases were removed entirely and one partially. The metastatic tumors were also thought to be of myxomatous origin by light and electron microscopy. Only at autopsy was it demonstrated that the patient had a primary chondrosarcoma of the pelvis giving rise to widespread myxoid metastases to the heart and other sites.", "PMID": 966789}
{"id": "pubmed23n0032_6884", "title": "Silent, chronic, massive pulmonary thromboembolism masquerading as bronchogenic carcinoma.", "content": "A unique case is described of a 64-year-old white woman who had silent thromboembolic occlusion of the right pulmonary artery. Over the ensuing months, severe pulmonary hypertension developed, as manifested by marked dilatation and atherosclerosis of the right and left pulmonary arteries and severe right ventricular hypertrophy. Nevertheless, she remained fully ambulatory and felt generally well throughout this time. Eventually, however, the pulmonary arteries became so dilated that they compressed the recurrent laryngeal nerve as it looped under the aortic arch, and it was the resulting hoarseness that first caused the patient to seek medical attention. A work-up disclosed normal peripheral lung fields on x-ray study and a large dense right hilar mass. Accordingly, the patient was subjected to an exploratory thoracotomy on the reasonable but mistaken diagnosis of bronchogenic carcinoma. After the following operation, her condition deteriorated. She developed bronchopneumonia which, when superimposed on her already precariously reduced cardiopulmonary function, precipitated respiratory insufficiency. An independent stroke was the immediate cause of death.", "contents": "Silent, chronic, massive pulmonary thromboembolism masquerading as bronchogenic carcinoma. A unique case is described of a 64-year-old white woman who had silent thromboembolic occlusion of the right pulmonary artery. Over the ensuing months, severe pulmonary hypertension developed, as manifested by marked dilatation and atherosclerosis of the right and left pulmonary arteries and severe right ventricular hypertrophy. Nevertheless, she remained fully ambulatory and felt generally well throughout this time. Eventually, however, the pulmonary arteries became so dilated that they compressed the recurrent laryngeal nerve as it looped under the aortic arch, and it was the resulting hoarseness that first caused the patient to seek medical attention. A work-up disclosed normal peripheral lung fields on x-ray study and a large dense right hilar mass. Accordingly, the patient was subjected to an exploratory thoracotomy on the reasonable but mistaken diagnosis of bronchogenic carcinoma. After the following operation, her condition deteriorated. She developed bronchopneumonia which, when superimposed on her already precariously reduced cardiopulmonary function, precipitated respiratory insufficiency. An independent stroke was the immediate cause of death.", "PMID": 966790}
{"id": "pubmed23n0032_6885", "title": "A new method of producing pulsatile flow during cardiopulmonary bypass using a standard roller pump.", "content": "A new method for producing pulsatile flow during cardiopulmonary bypass is described. The method requires only the addition of a dilated segment in the tubing which passes beneath the arc of a standard roller pump. Preliminary studies demonstrate that pulsatile flow and pressure with physiologic contours can be produced by this \"bubble tube\" system. Furthermore, the new tubing causes less hemolysis than standard pump tubing.", "contents": "A new method of producing pulsatile flow during cardiopulmonary bypass using a standard roller pump. A new method for producing pulsatile flow during cardiopulmonary bypass is described. The method requires only the addition of a dilated segment in the tubing which passes beneath the arc of a standard roller pump. Preliminary studies demonstrate that pulsatile flow and pressure with physiologic contours can be produced by this \"bubble tube\" system. Furthermore, the new tubing causes less hemolysis than standard pump tubing.", "PMID": 966791}
{"id": "pubmed23n0032_6886", "title": "The Waterston anastomosis with no deaths in the neonate.", "content": "The Waterston anastomosis has been performed with no deaths in 11 consecutive neonates during the past 24 months. Seven neonates had pulmonary atresia with either single ventricle or hypoplastic right ventricle, 3 had tetralogy of Fallot, and one had severe pulmonary stenosis with a normal right ventricle. Six neonates were one day old and only one was older than 7 days. All have obtained symptomatic and documented (increase Po2) benefit. This level of success is attributed to (1) minimal delay between the  recognition of cyanosis and operation (mean time between hospital admission and catheterization was 3 hours and between catheterization and operation, 4 hours); (2) correction of any base deficit prior to, during, and after operation; (3) accurate construction of the anastomosis to avoid excessive size, and (4) careful postoperative management of the pulmonary subsystem by experienced personnel. We have demonstrated that the high mortality rate previously reported for the Waterston anastomosis in the neonate can be markedly improved by an appropriate patient management program.", "contents": "The Waterston anastomosis with no deaths in the neonate. The Waterston anastomosis has been performed with no deaths in 11 consecutive neonates during the past 24 months. Seven neonates had pulmonary atresia with either single ventricle or hypoplastic right ventricle, 3 had tetralogy of Fallot, and one had severe pulmonary stenosis with a normal right ventricle. Six neonates were one day old and only one was older than 7 days. All have obtained symptomatic and documented (increase Po2) benefit. This level of success is attributed to (1) minimal delay between the  recognition of cyanosis and operation (mean time between hospital admission and catheterization was 3 hours and between catheterization and operation, 4 hours); (2) correction of any base deficit prior to, during, and after operation; (3) accurate construction of the anastomosis to avoid excessive size, and (4) careful postoperative management of the pulmonary subsystem by experienced personnel. We have demonstrated that the high mortality rate previously reported for the Waterston anastomosis in the neonate can be markedly improved by an appropriate patient management program.", "PMID": 966792}
{"id": "pubmed23n0032_6887", "title": "Surgical management of life-threatening coronary artery disease: experience in 18 consecutive patients with left main trunk or equivalent coronary artery obstruction associated with an obstructed or a nondominant right coronary artery.", "content": "Eighteen patients with obstruction of the left main trunk or equivalent coronary artery associated with an obstructed or a nondominant right coronary artery were studied. All patients had coronary artery bypass surgery because of their symptoms and coronary anatomy. Five patients had coronary artery bypass without the use of preoperative elective intra-aortic balloon counterpulsation, and 3 of these patients had cardiac arrest prior to insertion of the grafts. The other 13 patients received elective intra-aortic balloon counterpulsation prior to induction of anesthesia and cannulation. None in this second group of patients developed any hypotensive episodes or cardiac arrest, and all have done well following the bypass operation. This experience suggests that the use of elective preoperative intra-aortic balloon assist in patients who have a very critical coronary artery obstruction is indicated in view of the high incidence of cardiac arrest observed in patients who did not receive the preoperative elective intra-aortic balloon support.", "contents": "Surgical management of life-threatening coronary artery disease: experience in 18 consecutive patients with left main trunk or equivalent coronary artery obstruction associated with an obstructed or a nondominant right coronary artery. Eighteen patients with obstruction of the left main trunk or equivalent coronary artery associated with an obstructed or a nondominant right coronary artery were studied. All patients had coronary artery bypass surgery because of their symptoms and coronary anatomy. Five patients had coronary artery bypass without the use of preoperative elective intra-aortic balloon counterpulsation, and 3 of these patients had cardiac arrest prior to insertion of the grafts. The other 13 patients received elective intra-aortic balloon counterpulsation prior to induction of anesthesia and cannulation. None in this second group of patients developed any hypotensive episodes or cardiac arrest, and all have done well following the bypass operation. This experience suggests that the use of elective preoperative intra-aortic balloon assist in patients who have a very critical coronary artery obstruction is indicated in view of the high incidence of cardiac arrest observed in patients who did not receive the preoperative elective intra-aortic balloon support.", "PMID": 966793}
{"id": "pubmed23n0032_6888", "title": "Homograft replacement of the trachea using immunosuppression.", "content": "The experiments were divided into three-ring and five-ring autografts and allografts. In the animals receiving allografts, the trachea was exchanged between paired animals which were preoperatively immunosuppressed with Imuran (azathioprine) and postoperatively given Decadron (dexamethasone). Seven of the 14 dogs receiving a three-ring allograft survived until put to death months after the operation. Postmortem examination demonstrated a patent airway, but the cartilaginous rings were absent and the proximal and distal suture lines were approximated. By contrast, none of the dogs receiving the five-ring allografts survived beyond 29 days. In all, the cartilage had disappeared and the intervening fibrous tissue had collapsed and constricted the airway. On the other hand, in animals in which the trachea was simply transected and resutured over an area of seven to ten rings, leaving membranous portion intact, the trachea was entirely normal when the animals were put to death. It is concluded that tracheal replacement with an allograft is not feasible, even with immunosuppression. It is the blood supply to the grafted segment which precludes success.", "contents": "Homograft replacement of the trachea using immunosuppression. The experiments were divided into three-ring and five-ring autografts and allografts. In the animals receiving allografts, the trachea was exchanged between paired animals which were preoperatively immunosuppressed with Imuran (azathioprine) and postoperatively given Decadron (dexamethasone). Seven of the 14 dogs receiving a three-ring allograft survived until put to death months after the operation. Postmortem examination demonstrated a patent airway, but the cartilaginous rings were absent and the proximal and distal suture lines were approximated. By contrast, none of the dogs receiving the five-ring allografts survived beyond 29 days. In all, the cartilage had disappeared and the intervening fibrous tissue had collapsed and constricted the airway. On the other hand, in animals in which the trachea was simply transected and resutured over an area of seven to ten rings, leaving membranous portion intact, the trachea was entirely normal when the animals were put to death. It is concluded that tracheal replacement with an allograft is not feasible, even with immunosuppression. It is the blood supply to the grafted segment which precludes success.", "PMID": 966794}
{"id": "pubmed23n0032_6889", "title": "Gunshot wounds of the esophagus.", "content": "During a 4 year period between 1970 and 1974 there were eleven esophageal gunshot wounds representing 52 per cent of the total esophageal perforations. The increased incidence of esophageal gunshot wounds reflects the higher rate of civilian gunshot injuries. There were six perforations in the cervical portion of the esophagus and five in the thoracic portion, with three located above the aortic arch, one in the midesophagus, and one in the lower third. Symptoms are less diagnostic than in esophageal perforations from other causes because the gunshot wound tends to mask the complaints related to mediastinitis. The signs are similar. In 9 patients free air was present in the neck or mediastinum and in 10 patients the diagnosis was confirmed by barium swallow. Of the 2 patients without free air, one had a lateral thoracic wound and esophageal injury was not suspected; the diagnosis was made by drainage of oral feeding through a thoracostomy and confirmed by barium swallow. In the other patient the perforation was found during surgery for hemothorax. Primary repair with drainage was done in the group with cervical injuries. All survived with no serious complications. In the group with thoracic injuries, fistulas developed in 2 of 3 patients who had primary repair with drainage. Two patients with extensive injuries of the esophagus treated by defunctionalization did well but required a second procedure. It is concluded that gunshot wounds of the cervical esophagus, if treated promptly by suture and drainage, will do well. Thoracic injuries represent a more difficult problem and it is suggested that defunctionalization of the esophagus is the safest procedure, particularly if damage is extensive.", "contents": "Gunshot wounds of the esophagus. During a 4 year period between 1970 and 1974 there were eleven esophageal gunshot wounds representing 52 per cent of the total esophageal perforations. The increased incidence of esophageal gunshot wounds reflects the higher rate of civilian gunshot injuries. There were six perforations in the cervical portion of the esophagus and five in the thoracic portion, with three located above the aortic arch, one in the midesophagus, and one in the lower third. Symptoms are less diagnostic than in esophageal perforations from other causes because the gunshot wound tends to mask the complaints related to mediastinitis. The signs are similar. In 9 patients free air was present in the neck or mediastinum and in 10 patients the diagnosis was confirmed by barium swallow. Of the 2 patients without free air, one had a lateral thoracic wound and esophageal injury was not suspected; the diagnosis was made by drainage of oral feeding through a thoracostomy and confirmed by barium swallow. In the other patient the perforation was found during surgery for hemothorax. Primary repair with drainage was done in the group with cervical injuries. All survived with no serious complications. In the group with thoracic injuries, fistulas developed in 2 of 3 patients who had primary repair with drainage. Two patients with extensive injuries of the esophagus treated by defunctionalization did well but required a second procedure. It is concluded that gunshot wounds of the cervical esophagus, if treated promptly by suture and drainage, will do well. Thoracic injuries represent a more difficult problem and it is suggested that defunctionalization of the esophagus is the safest procedure, particularly if damage is extensive.", "PMID": 966795}
{"id": "pubmed23n0032_6890", "title": "Effect of thoracotomy upon the carotid sinus control of regional hemodynamics in the dog.", "content": "The effects of bilateral thoracotomy upon the operating characteristics of the carotid sinus reflex were examined in 11 vagotomized dogs. Pressure-flow relationships in the aorta, celiac, mesenteric, renal, and femoral arteries were studied as a function of pressure in the isolated carotid sinuses. These relationships were analyzed to give the carotid sinus reflex operating point values and sensitivities of pressure, flow, impedance, and power for each regional vascular bed. Following thoracotomy there was a significant fall in the closed-loop operating point pressure (CLOP) ( p less than 0.005). The operating point values and sensitivities for each regional flow and resistance together with the corresponding mean and oscillatory kinetic and pressure power were not significantly altered by thoracotomy. Renal characteristic impedance at the operating point significantly increased following thoracotomy (p less than 0.05), as did the aortic characteristic impedance for carotid sinus pressures (CSP) above the closed-loop operating point. The operating sensitivity of renal characteristic impedance was decreased (p less than 0.05). Possible mechanisms underlying these changes are discussed.", "contents": "Effect of thoracotomy upon the carotid sinus control of regional hemodynamics in the dog. The effects of bilateral thoracotomy upon the operating characteristics of the carotid sinus reflex were examined in 11 vagotomized dogs. Pressure-flow relationships in the aorta, celiac, mesenteric, renal, and femoral arteries were studied as a function of pressure in the isolated carotid sinuses. These relationships were analyzed to give the carotid sinus reflex operating point values and sensitivities of pressure, flow, impedance, and power for each regional vascular bed. Following thoracotomy there was a significant fall in the closed-loop operating point pressure (CLOP) ( p less than 0.005). The operating point values and sensitivities for each regional flow and resistance together with the corresponding mean and oscillatory kinetic and pressure power were not significantly altered by thoracotomy. Renal characteristic impedance at the operating point significantly increased following thoracotomy (p less than 0.05), as did the aortic characteristic impedance for carotid sinus pressures (CSP) above the closed-loop operating point. The operating sensitivity of renal characteristic impedance was decreased (p less than 0.05). Possible mechanisms underlying these changes are discussed.", "PMID": 966797}
{"id": "pubmed23n0032_6891", "title": "Anomalous origin of the right coronary artery from the pulmonary artery.", "content": "Anomalous origin of a coronary artery from the pulmonary artery is a relatively rarer congenital anomaly which almost exclusively involves the left coronary artery. A previously healthy 25-year-old woman had a cardiopulmonary arrest. Following resuscitation, investigations revealed an even rarer anomaly, an anomalous right coronary artery arising from the pulmonary artery. Successful surgical repair was accomplished by reimplanting the right coronary artery into the aortic root. The patient is currently asymptomatic 2 years postoperatively. The pertinent literature is reviewed.", "contents": "Anomalous origin of the right coronary artery from the pulmonary artery. Anomalous origin of a coronary artery from the pulmonary artery is a relatively rarer congenital anomaly which almost exclusively involves the left coronary artery. A previously healthy 25-year-old woman had a cardiopulmonary arrest. Following resuscitation, investigations revealed an even rarer anomaly, an anomalous right coronary artery arising from the pulmonary artery. Successful surgical repair was accomplished by reimplanting the right coronary artery into the aortic root. The patient is currently asymptomatic 2 years postoperatively. The pertinent literature is reviewed.", "PMID": 966798}
{"id": "pubmed23n0032_6892", "title": "Glucocorticoid protection of the myocardial cell membrane and the reduction of edema in experimental acute myocardial ischemia.", "content": "A possible protective effect of glucocorticoids on the ischemic myocardium was investigated in in situ dog hearts subjected to regional ischemia and in isolated rat hearts subjected to global ischemia. In the whole-animal preparation, the left anterior descending coronary artery (LAD) was occluded for 3 hours, or for 2 1/2 hours followed by 30 minutes of reperfusion. Dexamethasone phosphate was randomly administered (20 mg. per kilogram intravenously) after 15 minutes of ischemia. Its effects were studied on the following: (1) myocardial cell membrane integrity, using electron microscopic examination of tissue biopsies treated with colloidal lanthanum; (2) myocardial water content, measuring the wet/dry weight of myocardial tissue; (3) ischemic injury, by a count of fuchsinophilic cells at light microscopy. In isolated rat hearts, ischemia was produced by a 60 per cent reduction of coronary flow. Randomized hearts were perfused for 2 hours with dexamethasone, 15 mg. per milliliter in buffered salt solution. Study included determination of tissue water content and coronary vascular resistance. Lanthanum was confined to the extracellular spaces in normal dog myocardium, but it was found all intracellularly after 3 hours of ischemia or after reperfusion. This was associated with morphologic changes characteristic of irreversible cell injury. In the hearts treated with dexamethasone, lanthanum remained excluded from the cells, water content was less (p less than 0.005), and fuchsinophilia less severe (p less than 0.005). Likewise, water content was less (p less than 0.005) and the increase in coronary vascular resistance resulting from ischemia less severe (p less than 0.005) in the dexamethasone-treated isolated rat hearts. Thus dexamethasone administered in pharmacologic doses, early, appeared to stabilize the cell membrane, limit myocardial edema, and reduce the severity of injury, both during ischemia and upon reperfusion.", "contents": "Glucocorticoid protection of the myocardial cell membrane and the reduction of edema in experimental acute myocardial ischemia. A possible protective effect of glucocorticoids on the ischemic myocardium was investigated in in situ dog hearts subjected to regional ischemia and in isolated rat hearts subjected to global ischemia. In the whole-animal preparation, the left anterior descending coronary artery (LAD) was occluded for 3 hours, or for 2 1/2 hours followed by 30 minutes of reperfusion. Dexamethasone phosphate was randomly administered (20 mg. per kilogram intravenously) after 15 minutes of ischemia. Its effects were studied on the following: (1) myocardial cell membrane integrity, using electron microscopic examination of tissue biopsies treated with colloidal lanthanum; (2) myocardial water content, measuring the wet/dry weight of myocardial tissue; (3) ischemic injury, by a count of fuchsinophilic cells at light microscopy. In isolated rat hearts, ischemia was produced by a 60 per cent reduction of coronary flow. Randomized hearts were perfused for 2 hours with dexamethasone, 15 mg. per milliliter in buffered salt solution. Study included determination of tissue water content and coronary vascular resistance. Lanthanum was confined to the extracellular spaces in normal dog myocardium, but it was found all intracellularly after 3 hours of ischemia or after reperfusion. This was associated with morphologic changes characteristic of irreversible cell injury. In the hearts treated with dexamethasone, lanthanum remained excluded from the cells, water content was less (p less than 0.005), and fuchsinophilia less severe (p less than 0.005). Likewise, water content was less (p less than 0.005) and the increase in coronary vascular resistance resulting from ischemia less severe (p less than 0.005) in the dexamethasone-treated isolated rat hearts. Thus dexamethasone administered in pharmacologic doses, early, appeared to stabilize the cell membrane, limit myocardial edema, and reduce the severity of injury, both during ischemia and upon reperfusion.", "PMID": 966799}
{"id": "pubmed23n0032_6893", "title": "Cor triatriatum: masked by complex congenital cardiac anomalies.", "content": "The radiographic, angiographic, hemodynamic, and surgical features of 2 cases of cor triatriatum complicated by other cardiac anomalies are presented. In the first case, the patient had tetralogy of Fallot, the scimitar syndrome, and left ventricular obstruction. In the second case the patient had truncus arteriosus. The first case was revealed only after augmentation of pulmonary flow by an aorta-pulmonary artery anastomosis. In the second case, the diagnosis was retrospectively suggested by certain angiographic and hemodynamic clues. The prospective diagnosis was made only after a modified Rastelli procedure for truncus arteriosus. The difficulties encountered in making the diagnosis in both cases are discussed. It is stressed that a high index of suspicion, thorough accumulation and examination of hemodynamic data, and excellent angiograms and plain film radiography are all necessary to an early diagnosis of cor triatriatum when the latter is complicated by other congenital heart anomalies.", "contents": "Cor triatriatum: masked by complex congenital cardiac anomalies. The radiographic, angiographic, hemodynamic, and surgical features of 2 cases of cor triatriatum complicated by other cardiac anomalies are presented. In the first case, the patient had tetralogy of Fallot, the scimitar syndrome, and left ventricular obstruction. In the second case the patient had truncus arteriosus. The first case was revealed only after augmentation of pulmonary flow by an aorta-pulmonary artery anastomosis. In the second case, the diagnosis was retrospectively suggested by certain angiographic and hemodynamic clues. The prospective diagnosis was made only after a modified Rastelli procedure for truncus arteriosus. The difficulties encountered in making the diagnosis in both cases are discussed. It is stressed that a high index of suspicion, thorough accumulation and examination of hemodynamic data, and excellent angiograms and plain film radiography are all necessary to an early diagnosis of cor triatriatum when the latter is complicated by other congenital heart anomalies.", "PMID": 966800}
{"id": "pubmed23n0032_6894", "title": "Endocrine studies during the refeeding of young women with nutritional amenorrhea and infertility.", "content": "Serial endocrine studies were performed during the refeeding of a group of nine young women who presented with amenorrhea associated with injudicious dieting. No significant psychopathology was disclosed by the Lanyon Psychological Screening Inventory in any of the patients, six of whom were single and three married and infertile. The mean weight before refeeding was 81.6% of the normal for height. The mean initial serum follicle-stimulating hormone concentration was within the limits of normal for young women of reproductive age, but the serum luteinizing hormone (LH) concentration and the vaginal maturation score were low. As weight was regained, the serum LH concentration increased (the pooled r for LH versus percent normal weight = 0.85) and the vaginal maturation score rose (the pooled r for vaginal maturation score versus percent normal weight = 0.93). When a mean weight of 96.6% of normal was reached after a mean of 5.3 (range, 2 to 11) months of refeeding, spontaneous menses occurred. The initial cycle was ovulatory in two instances and anovulatory in five, as determined by basal body temperature patterns, plasma progesterone concentrations, and endometrial biopsies. With continued weight gain, menstruation became regular and the three married patients conceived.", "contents": "Endocrine studies during the refeeding of young women with nutritional amenorrhea and infertility. Serial endocrine studies were performed during the refeeding of a group of nine young women who presented with amenorrhea associated with injudicious dieting. No significant psychopathology was disclosed by the Lanyon Psychological Screening Inventory in any of the patients, six of whom were single and three married and infertile. The mean weight before refeeding was 81.6% of the normal for height. The mean initial serum follicle-stimulating hormone concentration was within the limits of normal for young women of reproductive age, but the serum luteinizing hormone (LH) concentration and the vaginal maturation score were low. As weight was regained, the serum LH concentration increased (the pooled r for LH versus percent normal weight = 0.85) and the vaginal maturation score rose (the pooled r for vaginal maturation score versus percent normal weight = 0.93). When a mean weight of 96.6% of normal was reached after a mean of 5.3 (range, 2 to 11) months of refeeding, spontaneous menses occurred. The initial cycle was ovulatory in two instances and anovulatory in five, as determined by basal body temperature patterns, plasma progesterone concentrations, and endometrial biopsies. With continued weight gain, menstruation became regular and the three married patients conceived.", "PMID": 966811}
{"id": "pubmed23n0032_6895", "title": "Hormones and preantral follicle growth in women.", "content": "Studies of follicular morphology in biopsies of human ovarian tissue obtained from women with a syndrome of hypogonadotropic hypogonadism have been reviewed. Follicular maturation was consistent with that seen in ovarian tissues removed at postmortem examination of infants. Similarly, follicular maturation was inhibited in ovaries of women receiving oral contraceptive preparations consisting of a mixture of an estrogen and a progestogen. Considered together, ovarian morphology in these two states of relative gonadotropin deficiency suggests that initiation and progression of follicular maturation require adequate quantities of gonadotropins. The stimulatory effects of gonadotropin on preantral stages of follicular maturation are mediated by estrogen in rat ovaries. Failure to observe follicular maturation in ovaries of women with a congenital inability to synthesize estrogen in response to stimulation by gonadotropin suggests that the steroid may play a similar role in initiating follicular maturation in women.", "contents": "Hormones and preantral follicle growth in women. Studies of follicular morphology in biopsies of human ovarian tissue obtained from women with a syndrome of hypogonadotropic hypogonadism have been reviewed. Follicular maturation was consistent with that seen in ovarian tissues removed at postmortem examination of infants. Similarly, follicular maturation was inhibited in ovaries of women receiving oral contraceptive preparations consisting of a mixture of an estrogen and a progestogen. Considered together, ovarian morphology in these two states of relative gonadotropin deficiency suggests that initiation and progression of follicular maturation require adequate quantities of gonadotropins. The stimulatory effects of gonadotropin on preantral stages of follicular maturation are mediated by estrogen in rat ovaries. Failure to observe follicular maturation in ovaries of women with a congenital inability to synthesize estrogen in response to stimulation by gonadotropin suggests that the steroid may play a similar role in initiating follicular maturation in women.", "PMID": 966812}
{"id": "pubmed23n0032_6896", "title": "Time study of effects of pregnant mare's serum gonadotropin on surface characteristics of granulosa cells in rat ovaries.", "content": "A single injection of pregnant mare's serum gonadotropin (PMSG) into immature female rats was used to stimulate graafian follicle growth. The surface of granulosa cells in the growing follicles was examined at 12-hour intervals for 72 hours by scanning electron microscopy. Between 24 and 36 hours after injection of PMSG, microvillous processes appeared on the surface of the cells. These villous processes became fully developed at 48 hours and remained for at least 72 hours after PMSG injection. The significance of this PMSG-induced microvilli formation on granulosa cells is discussed.", "contents": "Time study of effects of pregnant mare's serum gonadotropin on surface characteristics of granulosa cells in rat ovaries. A single injection of pregnant mare's serum gonadotropin (PMSG) into immature female rats was used to stimulate graafian follicle growth. The surface of granulosa cells in the growing follicles was examined at 12-hour intervals for 72 hours by scanning electron microscopy. Between 24 and 36 hours after injection of PMSG, microvillous processes appeared on the surface of the cells. These villous processes became fully developed at 48 hours and remained for at least 72 hours after PMSG injection. The significance of this PMSG-induced microvilli formation on granulosa cells is discussed.", "PMID": 966813}
{"id": "pubmed23n0032_6897", "title": "Some questions related to melanocyte-stimulating hormone.", "content": "A number of questions remain unsettled about the release of melanocyte-stimulating hormone (MSH) and about its function. Even though relatively few investigators are studying this area, some generalities have emerged during the last 10 years. It now seems that release of MSH from the pituitary is inhibited by a substance present in the hypothalamus. The structure of this physiologic inhibitor of MSH release may still not be considered an established entity but there is evidence for additional mechanisms capable of exerting a fine control on the release of MSH. Contrary to some opinions, the release of MSH does not always occur together with the release of ACTH, and the release of the two hormones can be dissociated in several laboratory and clinical situations. In addition, many studies have shown that the pituitary peptide, MSH, exerts behavioral and electroencephalographic effects in both the rat and man. The hypothalamic peptide Pro-Leu-Gly-NH2 (MIF-I) also has direct effects on the central nervous system that may include alleviation of the symptoms of Parkinson's disease.", "contents": "Some questions related to melanocyte-stimulating hormone. A number of questions remain unsettled about the release of melanocyte-stimulating hormone (MSH) and about its function. Even though relatively few investigators are studying this area, some generalities have emerged during the last 10 years. It now seems that release of MSH from the pituitary is inhibited by a substance present in the hypothalamus. The structure of this physiologic inhibitor of MSH release may still not be considered an established entity but there is evidence for additional mechanisms capable of exerting a fine control on the release of MSH. Contrary to some opinions, the release of MSH does not always occur together with the release of ACTH, and the release of the two hormones can be dissociated in several laboratory and clinical situations. In addition, many studies have shown that the pituitary peptide, MSH, exerts behavioral and electroencephalographic effects in both the rat and man. The hypothalamic peptide Pro-Leu-Gly-NH2 (MIF-I) also has direct effects on the central nervous system that may include alleviation of the symptoms of Parkinson's disease.", "PMID": 966814}
{"id": "pubmed23n0032_6898", "title": "The diagnosis and natural history of spinal cord arteriovenous malformations.", "content": "The present study of 71 patients shows that the initial symptoms often cannot differentiate spinal cord arteriovenous malformation from other lesions causing cord dysfunction, but the picture at the time of presentation may suggest the diagnosis. Most patients are males with neurologic findings referable to the thoracolumbar area who present with gradually progressive pain, weadness, sensory distubance, and disturbance of micturition. Early impairment of micturition may help suggest this lesion because it is less likely to be an early complaint in patients with disk disease or tumor affecting the spinal cord. Symptoms occasionally vary with posture and exercise and menses. Most commonly there are combined upper motor neuron and lower motor neuron manifestations with nonradicular sensory deficit. The cerebrospinal fluid is abnormal in more than 75% of cases. The myelogram is positivie in 75 to 90% of cases and the angiogram is almost always diagnostic.", "contents": "The diagnosis and natural history of spinal cord arteriovenous malformations. The present study of 71 patients shows that the initial symptoms often cannot differentiate spinal cord arteriovenous malformation from other lesions causing cord dysfunction, but the picture at the time of presentation may suggest the diagnosis. Most patients are males with neurologic findings referable to the thoracolumbar area who present with gradually progressive pain, weadness, sensory distubance, and disturbance of micturition. Early impairment of micturition may help suggest this lesion because it is less likely to be an early complaint in patients with disk disease or tumor affecting the spinal cord. Symptoms occasionally vary with posture and exercise and menses. Most commonly there are combined upper motor neuron and lower motor neuron manifestations with nonradicular sensory deficit. The cerebrospinal fluid is abnormal in more than 75% of cases. The myelogram is positivie in 75 to 90% of cases and the angiogram is almost always diagnostic.", "PMID": 966815}
{"id": "pubmed23n0032_6899", "title": "Intrasynovial injection of steroids uses and abuses.", "content": "Intra-articular injection of steroids is a valuable method of treatment, but selection of the appropriate clinical setting is required to obtain maximal benefits and to minimize the complications. All intra-articular injections must be performed under strict aseptic technique. The rheumatoid patient with one or two joints that resist systemic therapy and the patient with an occasional osteoarthritic joint with an acute inflammation are amenable to this treatment. The patient who has acute attacks of tendinitis, pseudogout, or bursitis will obtain significant benefits. Selected patients with traumatic and acute gouty arthritis also may benefit. Repetitive injections appear to be contra-indicated as they may create an environment conducive to joint destruction.", "contents": "Intrasynovial injection of steroids uses and abuses. Intra-articular injection of steroids is a valuable method of treatment, but selection of the appropriate clinical setting is required to obtain maximal benefits and to minimize the complications. All intra-articular injections must be performed under strict aseptic technique. The rheumatoid patient with one or two joints that resist systemic therapy and the patient with an occasional osteoarthritic joint with an acute inflammation are amenable to this treatment. The patient who has acute attacks of tendinitis, pseudogout, or bursitis will obtain significant benefits. Selected patients with traumatic and acute gouty arthritis also may benefit. Repetitive injections appear to be contra-indicated as they may create an environment conducive to joint destruction.", "PMID": 966816}
{"id": "pubmed23n0032_6900", "title": "Serum lipids in chronic renal failure.", "content": "The serum lipids and lipoprotein patterns in 100 adult patients with nonnephrotic chronic renal failure were analyzed retrospectively. Hypertriglyceridemia was found in 43% of these patients. Forty-nine of the 100 had a normal lipoprotein pattern, whereas 42 had type IV hyperlipoproteinemia. This abnormal lipoprotein pattern could not be correlated with the degree of renal impairment, the type of renal disease, or with the patient's age, sex, weight, or diet.", "contents": "Serum lipids in chronic renal failure. The serum lipids and lipoprotein patterns in 100 adult patients with nonnephrotic chronic renal failure were analyzed retrospectively. Hypertriglyceridemia was found in 43% of these patients. Forty-nine of the 100 had a normal lipoprotein pattern, whereas 42 had type IV hyperlipoproteinemia. This abnormal lipoprotein pattern could not be correlated with the degree of renal impairment, the type of renal disease, or with the patient's age, sex, weight, or diet.", "PMID": 966817}
{"id": "pubmed23n0032_6901", "title": "Development of the aging cell surface: a freeze-fracture analysis of gap junctions between human embryo fibroblasts aging in culture. A brief note.", "content": "Gap junctions have been identified between cells in late passage cultures of human embryo fibroblasts using techniques of freeze-fracture and electron microscopy. The junctions are sparsely distributed, which may suggest a decrease in electrotonic and metabolic coupling with increased age in culture.", "contents": "Development of the aging cell surface: a freeze-fracture analysis of gap junctions between human embryo fibroblasts aging in culture. A brief note. Gap junctions have been identified between cells in late passage cultures of human embryo fibroblasts using techniques of freeze-fracture and electron microscopy. The junctions are sparsely distributed, which may suggest a decrease in electrotonic and metabolic coupling with increased age in culture.", "PMID": 966823}
{"id": "pubmed23n0032_6902", "title": "Morphological changes in aging cell lines of Paramecium aurelia. II. Macronuclear alterations.", "content": "Electron microscopic examination of aging P. aurelia showed that the macronucleus undergoes characteristic structural changes. The surface of the aging macronucleus was highly invaginated. Compared with young macronuclei, the chromatin bodies appeared to be smaller and condensed, and were fewer per unit area of the aging macronucleus. The nucleolar bodies also showed morphological alterations. In many aging macronuclei the nucleolar bodies appeared to be aggregated. Large ring-shaped or coiled loops of nucleoli were also observed. These changes in the ultrastructure of macronucleus in the aging paramecia suggest that the macronucleus in the aging cells is less active in the synthesis of macromolecules and transport of material to the cytoplasm.", "contents": "Morphological changes in aging cell lines of Paramecium aurelia. II. Macronuclear alterations. Electron microscopic examination of aging P. aurelia showed that the macronucleus undergoes characteristic structural changes. The surface of the aging macronucleus was highly invaginated. Compared with young macronuclei, the chromatin bodies appeared to be smaller and condensed, and were fewer per unit area of the aging macronucleus. The nucleolar bodies also showed morphological alterations. In many aging macronuclei the nucleolar bodies appeared to be aggregated. Large ring-shaped or coiled loops of nucleoli were also observed. These changes in the ultrastructure of macronucleus in the aging paramecia suggest that the macronucleus in the aging cells is less active in the synthesis of macromolecules and transport of material to the cytoplasm.", "PMID": 966822}
{"id": "pubmed23n0032_6903", "title": "[Aerodynamic analysis of a new sterile operating enclosure, and comparative bacteriologic testing between a conventional operating theatre and a sterile enclosure during sham operations (author's transl)].", "content": "An aerodynamic and bacteriological evaluation of a new sterile operating enclosure has been undertaken, comparing it with a conventional operating theatre under sham operating conditions with or without coincident use of either a surgical helmet or surgical face mask. Statistical analysis of these studies show the bacterial level in the sterile enclosure at the wound site is significantly lower than in ordinary operating theatre conditions whereas the use of a helmet with evacuating ducts effects these findings insignificantly.", "contents": "[Aerodynamic analysis of a new sterile operating enclosure, and comparative bacteriologic testing between a conventional operating theatre and a sterile enclosure during sham operations (author's transl)]. An aerodynamic and bacteriological evaluation of a new sterile operating enclosure has been undertaken, comparing it with a conventional operating theatre under sham operating conditions with or without coincident use of either a surgical helmet or surgical face mask. Statistical analysis of these studies show the bacterial level in the sterile enclosure at the wound site is significantly lower than in ordinary operating theatre conditions whereas the use of a helmet with evacuating ducts effects these findings insignificantly.", "PMID": 966908}
{"id": "pubmed23n0032_6904", "title": "[Pathogenesis and clinics of gastric stump carcinoma (author's transl)].", "content": "Statistics of postmortem examinations and retrospective long-term observations indicate a disposition of the resected stomach for the development of a primary carcinoma. The risk to develop a carcinoma after resection for peptic ulcer shows a two- to three-fold increase compared to gastric cancer mortality of the normal population. Because of the lack of typical symptoms for gastric stump cancer surgical treatment will occur late and the 5-years survival rate is only 1.38%. The cause of the increased cancer risk after gastric resection seems to be the atrophic gastritis in the gastric remnant which is found after a few years.", "contents": "[Pathogenesis and clinics of gastric stump carcinoma (author's transl)]. Statistics of postmortem examinations and retrospective long-term observations indicate a disposition of the resected stomach for the development of a primary carcinoma. The risk to develop a carcinoma after resection for peptic ulcer shows a two- to three-fold increase compared to gastric cancer mortality of the normal population. Because of the lack of typical symptoms for gastric stump cancer surgical treatment will occur late and the 5-years survival rate is only 1.38%. The cause of the increased cancer risk after gastric resection seems to be the atrophic gastritis in the gastric remnant which is found after a few years.", "PMID": 966909}
{"id": "pubmed23n0032_6905", "title": "[Release of 14c-chloramphenicol in the rabbit femur implanted with polymethylmetacrylate (author's transl)].", "content": "In vitro studies and animal experiments were started for the purpose of following the migration of chloramphenicol marked with 14C from polymerised polymethylmetacrylate cylinders. Test-cylinders were submerged in a physiological saline solution and the 14C concentration followed over a period of 34 days. Two series were started with the cylinders being submerged at intervals of 5 and 40 min after the start of polymerisation. These in vitro studies showed that initially the migration of active substances marked with 14C from the plastic cylinders was extremely high but remained then constant over the whole test period. Over a period of at least 20 days a higher 14C migration was evident in those cylinders submerged in the elution 5 min after the start of polymerisation. In our in vivo studies we implanted test-cylinders into the femur of rabbits. The migration of active substances marked with 14C resulted in 14C-concentrations being present in the surrounding tissues. During the phase of exudation, depending on the operative process, the concentration increased and reached values that remained constant during the phase of recanalisation of the surrounding tissues over a period of up to 6 weeks. Thereafter the concentration of active substances fell. The max. concentration rates of active substances within the boundary layer were between 15 and 20 mug/g humidity weight. After 8 weeks we found concentration rates of approx. 1 mug/g humidity weight. On examining the 14C distribution within the implanted plastic cylinders we observed that in the course of time the boundary layer increased in thickness and a concentration gradient towards the centre had developed. The spread of diffusion gradually seized the deeper layers so that a long-lasting presence of active substances may be expected.", "contents": "[Release of 14c-chloramphenicol in the rabbit femur implanted with polymethylmetacrylate (author's transl)]. In vitro studies and animal experiments were started for the purpose of following the migration of chloramphenicol marked with 14C from polymerised polymethylmetacrylate cylinders. Test-cylinders were submerged in a physiological saline solution and the 14C concentration followed over a period of 34 days. Two series were started with the cylinders being submerged at intervals of 5 and 40 min after the start of polymerisation. These in vitro studies showed that initially the migration of active substances marked with 14C from the plastic cylinders was extremely high but remained then constant over the whole test period. Over a period of at least 20 days a higher 14C migration was evident in those cylinders submerged in the elution 5 min after the start of polymerisation. In our in vivo studies we implanted test-cylinders into the femur of rabbits. The migration of active substances marked with 14C resulted in 14C-concentrations being present in the surrounding tissues. During the phase of exudation, depending on the operative process, the concentration increased and reached values that remained constant during the phase of recanalisation of the surrounding tissues over a period of up to 6 weeks. Thereafter the concentration of active substances fell. The max. concentration rates of active substances within the boundary layer were between 15 and 20 mug/g humidity weight. After 8 weeks we found concentration rates of approx. 1 mug/g humidity weight. On examining the 14C distribution within the implanted plastic cylinders we observed that in the course of time the boundary layer increased in thickness and a concentration gradient towards the centre had developed. The spread of diffusion gradually seized the deeper layers so that a long-lasting presence of active substances may be expected.", "PMID": 966910}
{"id": "pubmed23n0032_6906", "title": "[Hydatid disease of liver and its complications (author's transl)].", "content": "This is a report on 121 cases of hydatid disease of liver. The alteration of the false cyst, explaining the most common complications of the disease, is discussed. Complications were present in 61 (50.41%) of our 121 patients: calcification in 13.22%, infection in 10.74%, perforation in bile ducts in 9.91%, thorax in 5.78% and abdominal cavity in 10.74% of our cases. Diagnostic importances of clinical findings, immunoelectrophoretic studies and radioisotopic liverscanning is emphasized. In 28 cases the complete excision of cysts were carried out, in 110 cases only removal of the hydatid membrane was performed. Other therapeutic methods are discussed. Of 108 treated patients 7 died postoperatively: they were all over 70 years old.", "contents": "[Hydatid disease of liver and its complications (author's transl)]. This is a report on 121 cases of hydatid disease of liver. The alteration of the false cyst, explaining the most common complications of the disease, is discussed. Complications were present in 61 (50.41%) of our 121 patients: calcification in 13.22%, infection in 10.74%, perforation in bile ducts in 9.91%, thorax in 5.78% and abdominal cavity in 10.74% of our cases. Diagnostic importances of clinical findings, immunoelectrophoretic studies and radioisotopic liverscanning is emphasized. In 28 cases the complete excision of cysts were carried out, in 110 cases only removal of the hydatid membrane was performed. Other therapeutic methods are discussed. Of 108 treated patients 7 died postoperatively: they were all over 70 years old.", "PMID": 966911}
{"id": "pubmed23n0032_6907", "title": "Division of posterior cricoid plate in young children with subglottic stenosis.", "content": "Subglottic stenosis was corrected surgically in three young children. The key surgical maneuver is the anterior and posterior division of the cricoid cartilage. Endoscopic, xerographic and speech assessments have been done at intervals since decannulation. The anterior commissure is widened and the endolarynx deformed in two children examined. Radiographic studies indicated that increased airway diameters have been maintained to date. Speech has been adversely affected to some degree in all three patients.", "contents": "Division of posterior cricoid plate in young children with subglottic stenosis. Subglottic stenosis was corrected surgically in three young children. The key surgical maneuver is the anterior and posterior division of the cricoid cartilage. Endoscopic, xerographic and speech assessments have been done at intervals since decannulation. The anterior commissure is widened and the endolarynx deformed in two children examined. Radiographic studies indicated that increased airway diameters have been maintained to date. Speech has been adversely affected to some degree in all three patients.", "PMID": 966912}
{"id": "pubmed23n0032_6908", "title": "Bifid epiglottis: a rare laryngeal anomaly.", "content": "Two cases of bifid epiglottis are presented: one with an associated laryngeal cyst and another with an associated cricoid stenosis. The occurrence of multiple laryngeal anomalies in association with bifid epiglottis has not previously been described. The occurrence of an extra digit is noted to be statistically significant both in the current series and in a review of the literature. A brief review of the embryologic classification and staging by the Carnegie System, and the correlation of the time sequence of development of the epiglottis is presented. No correlation is made as to the mechanism of the origin of this laryngeal anomaly, as adequate embryologic knowledge of the development of the pharynx is not available at this time.", "contents": "Bifid epiglottis: a rare laryngeal anomaly. Two cases of bifid epiglottis are presented: one with an associated laryngeal cyst and another with an associated cricoid stenosis. The occurrence of multiple laryngeal anomalies in association with bifid epiglottis has not previously been described. The occurrence of an extra digit is noted to be statistically significant both in the current series and in a review of the literature. A brief review of the embryologic classification and staging by the Carnegie System, and the correlation of the time sequence of development of the epiglottis is presented. No correlation is made as to the mechanism of the origin of this laryngeal anomaly, as adequate embryologic knowledge of the development of the pharynx is not available at this time.", "PMID": 966913}
{"id": "pubmed23n0032_6909", "title": "Patterns of anomalies in children with malformed ears.", "content": "Sixteen children with anomalies of the auricle and/or middle ear who presented malformations of the face, mouth, upper airway, spine, limbs, heart, gastrointestinal (GI), and/or genitourinary (GU) systems, were described. While clusters of anomalies suggested syndromes such as the oculo-auriculo-vertebral syndrome of Goldenhar, hamifacial microsomia, mandibulo-facial dysostosis (Treacher Collins syndrome), Pierre Robin, Klippel-Feil, Moebius, Duane, and/or VATER syndromes, many children did not fit what are usually considered even minimal criteria for these syndromes. Several children had malformations which fit the description of more than one syndrome. The importance of investigating the children for unsuspected anomalies, especially of the GU system, was emphasized. Life threatening problems in this group consisted of airway problems, congenital heart disease, and major anomalies of the GI and GU systems. Better management of sucking, swallowing and airway problems might have decreased the early morbidity and mortality (3/16) in this group. Children with multiple defacing anomalies may not be mentally retarded so that aggressive management of their visceral anomalies and hearing problems, and early educational intervention are mandatory. Delay in development may be due to hearing loss, vestibular impairment, ataxia, the consequences of early malnutrition, and multiple hospitalizations rather than to mental retardation. A pessimistic attitude in infancy is unwarranted since it is impossible to predict which children will end up competitive individuals.", "contents": "Patterns of anomalies in children with malformed ears. Sixteen children with anomalies of the auricle and/or middle ear who presented malformations of the face, mouth, upper airway, spine, limbs, heart, gastrointestinal (GI), and/or genitourinary (GU) systems, were described. While clusters of anomalies suggested syndromes such as the oculo-auriculo-vertebral syndrome of Goldenhar, hamifacial microsomia, mandibulo-facial dysostosis (Treacher Collins syndrome), Pierre Robin, Klippel-Feil, Moebius, Duane, and/or VATER syndromes, many children did not fit what are usually considered even minimal criteria for these syndromes. Several children had malformations which fit the description of more than one syndrome. The importance of investigating the children for unsuspected anomalies, especially of the GU system, was emphasized. Life threatening problems in this group consisted of airway problems, congenital heart disease, and major anomalies of the GI and GU systems. Better management of sucking, swallowing and airway problems might have decreased the early morbidity and mortality (3/16) in this group. Children with multiple defacing anomalies may not be mentally retarded so that aggressive management of their visceral anomalies and hearing problems, and early educational intervention are mandatory. Delay in development may be due to hearing loss, vestibular impairment, ataxia, the consequences of early malnutrition, and multiple hospitalizations rather than to mental retardation. A pessimistic attitude in infancy is unwarranted since it is impossible to predict which children will end up competitive individuals.", "PMID": 966914}
{"id": "pubmed23n0032_6910", "title": "Recurrent meningitis secondary to idiopathic oval window CSF leak.", "content": "Bacterial meningitis remains a life-threatening infection even in the present antibiotic era; thus, any abnormality which predisposes a patient to a recurrence of this serious disease, must be identified and corrected. This report describes the histroy of a 12-year old boy with a profound neurosensory hearing loss, a related absence of vestibular function and a Mondini-type of temporal bone dysplasia who developed recurrent episodes of meningitis which were due to an idiopathic cerebrospinal fluid otorrhea. Even though the meningitis was labyrinthogenic in origin, the patient did not experience the associated symptoms of hearing loss and/or vertigo since the affected inner ear was clinically unreactive. By surgically exploring the middle ear, the presence of a cerebrospinal fluid otorrhea was confirmed. The leak was observed to be coming from a defect in the stapes footplate, and it was controlled by firmly packing the inner ear vestibule with muscle. A remarkable similarity exists between the patient described above and the 15 previously reported cases of meningitis due to a spontaneous cerebrospinal fluid otorrhea. Generally, the problem occurred in young children, the average age being 6.4 years; male and female were equally afflicted. All 15 previously reported cases had a severe neurosensory hearing loss which was unilateral in 10 individuals and bilateral in the other five. In 11 of the case reports, the vestibular function was evaluated, and the labyrinth was noted to be unreactive in the affected ear. An associated congenital abnormality of the inner ear was described in 11 of the patients reviewed. Anatomically, in 13 cases, the leak was observed to be coming from the oval window area. Other affected sites included one report of a fissure of the promontory and one report of a defect in the roof of the eustachian tube. Multiple surgical procedures were required in 11 of the 15 patients in order to identify the exact source of the otorrhea and to seal it permanently. In three cases, the successful procedure was a middle ear exploration with stapedectomy and packing of the inner ear vestibule. Overall, a total of 36 operations was performed in the 15 patients reviewed. In conclusion, when the physician is confronted by a case of meningitis in a patient with a unilateral or bilateral total loss of hearing and vestibular function, the possible presence of an idiopathic cerebrospinal fluid leak should be considered, expecially if radiographic studies demonstrate a temporal bone dysplasia. In these selected cases, if the etiology of the meningitis is obscure, a middle ear exploration should be performed both for diagnostic purposes as a means to ascertain definitely the presence of a leak and for therapeutic purposes to seal it effectively.", "contents": "Recurrent meningitis secondary to idiopathic oval window CSF leak. Bacterial meningitis remains a life-threatening infection even in the present antibiotic era; thus, any abnormality which predisposes a patient to a recurrence of this serious disease, must be identified and corrected. This report describes the histroy of a 12-year old boy with a profound neurosensory hearing loss, a related absence of vestibular function and a Mondini-type of temporal bone dysplasia who developed recurrent episodes of meningitis which were due to an idiopathic cerebrospinal fluid otorrhea. Even though the meningitis was labyrinthogenic in origin, the patient did not experience the associated symptoms of hearing loss and/or vertigo since the affected inner ear was clinically unreactive. By surgically exploring the middle ear, the presence of a cerebrospinal fluid otorrhea was confirmed. The leak was observed to be coming from a defect in the stapes footplate, and it was controlled by firmly packing the inner ear vestibule with muscle. A remarkable similarity exists between the patient described above and the 15 previously reported cases of meningitis due to a spontaneous cerebrospinal fluid otorrhea. Generally, the problem occurred in young children, the average age being 6.4 years; male and female were equally afflicted. All 15 previously reported cases had a severe neurosensory hearing loss which was unilateral in 10 individuals and bilateral in the other five. In 11 of the case reports, the vestibular function was evaluated, and the labyrinth was noted to be unreactive in the affected ear. An associated congenital abnormality of the inner ear was described in 11 of the patients reviewed. Anatomically, in 13 cases, the leak was observed to be coming from the oval window area. Other affected sites included one report of a fissure of the promontory and one report of a defect in the roof of the eustachian tube. Multiple surgical procedures were required in 11 of the 15 patients in order to identify the exact source of the otorrhea and to seal it permanently. In three cases, the successful procedure was a middle ear exploration with stapedectomy and packing of the inner ear vestibule. Overall, a total of 36 operations was performed in the 15 patients reviewed. In conclusion, when the physician is confronted by a case of meningitis in a patient with a unilateral or bilateral total loss of hearing and vestibular function, the possible presence of an idiopathic cerebrospinal fluid leak should be considered, expecially if radiographic studies demonstrate a temporal bone dysplasia. In these selected cases, if the etiology of the meningitis is obscure, a middle ear exploration should be performed both for diagnostic purposes as a means to ascertain definitely the presence of a leak and for therapeutic purposes to seal it effectively.", "PMID": 966915}
{"id": "pubmed23n0032_6911", "title": "Office otology follow-up.", "content": "The validation of otological care in the previous quarter century has been based, by and large, on short-term follow-up information. The rapid variation in procedures introduced during this period indicates that there may be need for further consideration. Analyses based on relatively short duration have led the \"acceptance\" of a variety of diagnostic and treatment methods which have proven disappointing in some cases. To help avoid this invalid \"acceptance,\" a review of patients with significant follow-up may result in a more critical evaluation of future procedural changes. This presentation will be in four parts: 1. History of the office structure. 2. Discussion of five long-term patients that illustrates and supports certain otological concepts. 3. A review of surgical care for chronic suppurative otitis media in 1968. 4. A review of patients treated for serous otitis media with myringotomy and tubal insertion in 1968.", "contents": "Office otology follow-up. The validation of otological care in the previous quarter century has been based, by and large, on short-term follow-up information. The rapid variation in procedures introduced during this period indicates that there may be need for further consideration. Analyses based on relatively short duration have led the \"acceptance\" of a variety of diagnostic and treatment methods which have proven disappointing in some cases. To help avoid this invalid \"acceptance,\" a review of patients with significant follow-up may result in a more critical evaluation of future procedural changes. This presentation will be in four parts: 1. History of the office structure. 2. Discussion of five long-term patients that illustrates and supports certain otological concepts. 3. A review of surgical care for chronic suppurative otitis media in 1968. 4. A review of patients treated for serous otitis media with myringotomy and tubal insertion in 1968.", "PMID": 966916}
{"id": "pubmed23n0032_6912", "title": "Physician and patient-induced diseases in otolaryngology office practice.", "content": "\"Theragenic\" diseases produce common ENT complaints. They are frequently and predictably induced by self or physician treatment. Fifteen percent of first office visits in various practice situations stem from therapeutically-induced disease. Self treatment by cotton tip applicator ear cleaning, nasal decongestants, mouth washes, and lozenges produce symptoms which mimic other etiologies. Prescription medications produce diseases with ENT symptoms which are based on pharmacologic toxicity, multiple drug therapy, drug intolerance, and occasional idiopathic hypersensitivity. The treatment of these diseases is simple and effective once their etiology is discovered.", "contents": "Physician and patient-induced diseases in otolaryngology office practice. \"Theragenic\" diseases produce common ENT complaints. They are frequently and predictably induced by self or physician treatment. Fifteen percent of first office visits in various practice situations stem from therapeutically-induced disease. Self treatment by cotton tip applicator ear cleaning, nasal decongestants, mouth washes, and lozenges produce symptoms which mimic other etiologies. Prescription medications produce diseases with ENT symptoms which are based on pharmacologic toxicity, multiple drug therapy, drug intolerance, and occasional idiopathic hypersensitivity. The treatment of these diseases is simple and effective once their etiology is discovered.", "PMID": 966917}
{"id": "pubmed23n0032_6913", "title": "Pharyngoesophageal dysphagia and gastroesophageal reflux.", "content": "Pharyngoesophageal dysphagia occurred in 51.3 percent of 1,000 consecutive patients with gastroesophageal reflux. Aspiration, secondary to food obstruction, occurred in 30 percent of these patients, and some developed significant secondary respiratory symptoms. The site of obstruction was localized to the cricopharyngeus by timing the interval from swallow to obstruction. Cricopharyngeal incoordination was demonstrated in 20 of 52 patients studied by high speed esophageal manometry. Surgical correction of gastroesophageal reflux in patients with intractable reflux symptoms was shown to be effective in relieving pharyngoesophageal dysphagia in all but a small number of patients with very severe symptoms. In those with persistent dysphagia cricopharyngeal myotomy at a later stage was effective in giving relief.", "contents": "Pharyngoesophageal dysphagia and gastroesophageal reflux. Pharyngoesophageal dysphagia occurred in 51.3 percent of 1,000 consecutive patients with gastroesophageal reflux. Aspiration, secondary to food obstruction, occurred in 30 percent of these patients, and some developed significant secondary respiratory symptoms. The site of obstruction was localized to the cricopharyngeus by timing the interval from swallow to obstruction. Cricopharyngeal incoordination was demonstrated in 20 of 52 patients studied by high speed esophageal manometry. Surgical correction of gastroesophageal reflux in patients with intractable reflux symptoms was shown to be effective in relieving pharyngoesophageal dysphagia in all but a small number of patients with very severe symptoms. In those with persistent dysphagia cricopharyngeal myotomy at a later stage was effective in giving relief.", "PMID": 966918}
{"id": "pubmed23n0032_6914", "title": "Globus hystericus -- office evaluation by phychological testing with the MMPI.", "content": "Ninety-nine patients, twenty-three men and seventy-six women, who complained of a lump in the throat were evaluated with the Minnesota Multiphasic Personality Inventory, which revealed that these patients had a higher than average score on the depression and the hypochondriasis scales. The intensity of this finding was greater in males. One must never overlook the possibility of a neurotic patient also having serious organic disease.", "contents": "Globus hystericus -- office evaluation by phychological testing with the MMPI. Ninety-nine patients, twenty-three men and seventy-six women, who complained of a lump in the throat were evaluated with the Minnesota Multiphasic Personality Inventory, which revealed that these patients had a higher than average score on the depression and the hypochondriasis scales. The intensity of this finding was greater in males. One must never overlook the possibility of a neurotic patient also having serious organic disease.", "PMID": 966919}
{"id": "pubmed23n0032_6915", "title": "Computerized tomography (CT) in otolaryngology.", "content": "Computerized tomography (CT) of the brain has been available since 1973 and has changed neurological, neurosurgical and radiological practice beyond recognition. A rapid growth of literature has documented it role in the diagnosis of intracranial hematoma, cerebral atrophy, brain tumor, orbital lesions and postoperative tumor evaluation. This computerized method of image reconstruction is now also being applied to the rest of the body and to other diagnostic modalities such as isotopes and ultrasound. The initial impact of CT scanning in otolaryngology is largely in four areas: 1. Facial deformities either acquired or congenital as hypertelorism, fibrous dysplasia, ossifying fibroma and Crouzon's disease. 2. Tumors involving sinuses primarily or by secondary invasion. 3. Cerebello-pontine angle lesions as meningiomas, acoustic neuromas, and glomus jugulare tumors. The larger acoustic neuroma is well visualized by this technique rendering contrast myelography unnecessary. In CT scanning, subtle differences in position and an increase in density before contrast enhancement should allow a differentiation of angle meningiomas from acoustic neuromas; in addition, CT scanning has a very important role in the postoperative assessment of angle tumors. 4. Infections as cerebral abscess or cerebritis secondary to sinus or mastoid disease. The future holds changes that will allow much finer detail, re-orientation of the horizontal information into any plane, and much more accurate differentiation of tissue density. The principle of CT image reconstruction is being applied to both isotope and ultrasound scanning and should improve their accuracy and yield. If the rapid growth of the new technology over the past two years continues, even greater usefulness can be anticipated.", "contents": "Computerized tomography (CT) in otolaryngology. Computerized tomography (CT) of the brain has been available since 1973 and has changed neurological, neurosurgical and radiological practice beyond recognition. A rapid growth of literature has documented it role in the diagnosis of intracranial hematoma, cerebral atrophy, brain tumor, orbital lesions and postoperative tumor evaluation. This computerized method of image reconstruction is now also being applied to the rest of the body and to other diagnostic modalities such as isotopes and ultrasound. The initial impact of CT scanning in otolaryngology is largely in four areas: 1. Facial deformities either acquired or congenital as hypertelorism, fibrous dysplasia, ossifying fibroma and Crouzon's disease. 2. Tumors involving sinuses primarily or by secondary invasion. 3. Cerebello-pontine angle lesions as meningiomas, acoustic neuromas, and glomus jugulare tumors. The larger acoustic neuroma is well visualized by this technique rendering contrast myelography unnecessary. In CT scanning, subtle differences in position and an increase in density before contrast enhancement should allow a differentiation of angle meningiomas from acoustic neuromas; in addition, CT scanning has a very important role in the postoperative assessment of angle tumors. 4. Infections as cerebral abscess or cerebritis secondary to sinus or mastoid disease. The future holds changes that will allow much finer detail, re-orientation of the horizontal information into any plane, and much more accurate differentiation of tissue density. The principle of CT image reconstruction is being applied to both isotope and ultrasound scanning and should improve their accuracy and yield. If the rapid growth of the new technology over the past two years continues, even greater usefulness can be anticipated.", "PMID": 966920}
{"id": "pubmed23n0032_6916", "title": "Hemilaryngectomy for T3 (fixed cord) epidermoid carcinoma of larynx.", "content": "This clinicopathologic study was undertaken to determine whether true vocal cord fixation produced by epidermoid carcinoma is an absolute contra-indication to treatment by hemilaryngectomy. In a consecutive series of 114 hemilaryngectomies performed at McMillan Hospital (1960-1967) for previously untreated epidermoid carcinoma, 18 patients had fixation of the involved true vocal cord. Each hemilaryngectomy specimen (serial step sections in the longitudinal plane) was re-examined to ascertain the cause of vocal cord fixation; the adequacy of margin; and the presence of blood vessel, nerve sheath and cartilage invasion. Clinical follow-up on each patient was current through December, 1972 (5-12 years postop). All of these cases were seen initially by one of the authors (J.H.O.). Serial sections revealed that true vocal cord fixation was caused by muscle invasion in 14 of the 18 patients. \"Positive margins\" were present in eight patients but no immediate treatment was given. Two of these patients developed biopsy proven local recurrences, and both were cured with Co60 irradiation. Among the 18 patients with T3 epidermoid carcinoma treated by hemilaryngectomy: a. Three local recurrences developed, all in the anterior commissure. Two were cured with irradiation. The third had a laryngectomy but died from persistent cancer. b. Two patients developed cervical metastases (without local recurrence), and one was salvaged with radical neck dissection. c. Four patients died of other causes, cancer free, three to five years postoperatively. Of the 14 determinant patients, two patients died of cancer. Twelve (85 percent) were alive and free of cancer five years postoperatively. Two had received full course irradiation; one had a radical neck dissection, and all 12 had a functioning larynx. When Ogura's patients are added to the reports of other hemilaryngectomies performed despite true vocal cord fixation (Leroux-Robert [1950] 18/24, Kirchner, Som [1971] 13/19), a determinant salvage rate of 78 percent can be expected. True vocal cord fixation is generally caused by invasion of the vocalis muscle and thus may be well encompassed by a hemilaryngectomy. The precise anatomical limits of the lesion should dictate the type of surgery required for cure.", "contents": "Hemilaryngectomy for T3 (fixed cord) epidermoid carcinoma of larynx. This clinicopathologic study was undertaken to determine whether true vocal cord fixation produced by epidermoid carcinoma is an absolute contra-indication to treatment by hemilaryngectomy. In a consecutive series of 114 hemilaryngectomies performed at McMillan Hospital (1960-1967) for previously untreated epidermoid carcinoma, 18 patients had fixation of the involved true vocal cord. Each hemilaryngectomy specimen (serial step sections in the longitudinal plane) was re-examined to ascertain the cause of vocal cord fixation; the adequacy of margin; and the presence of blood vessel, nerve sheath and cartilage invasion. Clinical follow-up on each patient was current through December, 1972 (5-12 years postop). All of these cases were seen initially by one of the authors (J.H.O.). Serial sections revealed that true vocal cord fixation was caused by muscle invasion in 14 of the 18 patients. \"Positive margins\" were present in eight patients but no immediate treatment was given. Two of these patients developed biopsy proven local recurrences, and both were cured with Co60 irradiation. Among the 18 patients with T3 epidermoid carcinoma treated by hemilaryngectomy: a. Three local recurrences developed, all in the anterior commissure. Two were cured with irradiation. The third had a laryngectomy but died from persistent cancer. b. Two patients developed cervical metastases (without local recurrence), and one was salvaged with radical neck dissection. c. Four patients died of other causes, cancer free, three to five years postoperatively. Of the 14 determinant patients, two patients died of cancer. Twelve (85 percent) were alive and free of cancer five years postoperatively. Two had received full course irradiation; one had a radical neck dissection, and all 12 had a functioning larynx. When Ogura's patients are added to the reports of other hemilaryngectomies performed despite true vocal cord fixation (Leroux-Robert [1950] 18/24, Kirchner, Som [1971] 13/19), a determinant salvage rate of 78 percent can be expected. True vocal cord fixation is generally caused by invasion of the vocalis muscle and thus may be well encompassed by a hemilaryngectomy. The precise anatomical limits of the lesion should dictate the type of surgery required for cure.", "PMID": 966921}
{"id": "pubmed23n0032_6917", "title": "Red chorda tympani nerve in Herpes Zoster oticus.", "content": "A red chorda tympani nerve was previously reported as a finding limited to patients with Bell's palsy. This is a report of red chorda tympani nerve observed in 3 of 10 patients with Herpes Zoster oticus in whom the chorda tympani was visible. It is speculated that the red discoloration is due to a viral inflammatory process and, therefore, when this sign is present in a patient with idiopathic peripheral facial paralysis, a viral neuropathy should be suspected.", "contents": "Red chorda tympani nerve in Herpes Zoster oticus. A red chorda tympani nerve was previously reported as a finding limited to patients with Bell's palsy. This is a report of red chorda tympani nerve observed in 3 of 10 patients with Herpes Zoster oticus in whom the chorda tympani was visible. It is speculated that the red discoloration is due to a viral inflammatory process and, therefore, when this sign is present in a patient with idiopathic peripheral facial paralysis, a viral neuropathy should be suspected.", "PMID": 966922}
{"id": "pubmed23n0032_6918", "title": "Diagnostic value of tomography in unilateral vocal cord paralysis.", "content": "Tomographic studies were made on 56 patients with unilateral paralysis of the vocal cord. The findings were examined in relation to etiology, course, and laryngoscopic findings. In 74.1 percent of the cases, marked enlargement of the ventricle was noticed on the side of the paralysis, especially during inhalation. The paralyzed vocal cord was higher than the intact cord during phonation in 46.4 percent of the cases. The rest of the cases (53.6 percent) demonstrated no level difference between the vocal cords. The position of the paralyzed vocal cord, unilateral involvement of the cricothyroid muscle and other neck muscles were suggested as possible contributors to the findings.", "contents": "Diagnostic value of tomography in unilateral vocal cord paralysis. Tomographic studies were made on 56 patients with unilateral paralysis of the vocal cord. The findings were examined in relation to etiology, course, and laryngoscopic findings. In 74.1 percent of the cases, marked enlargement of the ventricle was noticed on the side of the paralysis, especially during inhalation. The paralyzed vocal cord was higher than the intact cord during phonation in 46.4 percent of the cases. The rest of the cases (53.6 percent) demonstrated no level difference between the vocal cords. The position of the paralyzed vocal cord, unilateral involvement of the cricothyroid muscle and other neck muscles were suggested as possible contributors to the findings.", "PMID": 966923}
{"id": "pubmed23n0032_6919", "title": "Rhinosporidiosis.", "content": "A case of rhinosporidiosis occurring in an immigrant from India is presented along with a review of the disease. Rhinosporidiosis most commonly involves the nose, and presents as a friable papillomatous mass causing nasal obstruction, epistaxis, purulent discharge, and headache may also occur. The treatment of choice is surgical excision with cauterization of the base.", "contents": "Rhinosporidiosis. A case of rhinosporidiosis occurring in an immigrant from India is presented along with a review of the disease. Rhinosporidiosis most commonly involves the nose, and presents as a friable papillomatous mass causing nasal obstruction, epistaxis, purulent discharge, and headache may also occur. The treatment of choice is surgical excision with cauterization of the base.", "PMID": 966924}
{"id": "pubmed23n0032_6920", "title": "Medullary carcinoma of the thyroid.", "content": "Medullary carcinoma is an unusual malignant neoplasm. The cell of origin is considered to be the C-cell which is derived from the ultimobranchial body and neural crest. The tumor is solid and has characteristic amyloid in the stroma. The lesion usually presents as a neck mass, and multicentricity is not unusual. In some patients diarrhea may be an important presenting symptom. Serum calcitonin is typically elevated and is important in the diagnostic confirmation. About 10 percent of the cases are part of a familial endocrine syndrome which includes pheochromocytomas, mucosal neuromas, and other endocrine gland adenomas. Frequent multicentricity and early cervical lymphatic and systemic metastases necessitate total thyroidectomy and radical neck dissection. Prognosis varies from a rapid demise to long term survival.", "contents": "Medullary carcinoma of the thyroid. Medullary carcinoma is an unusual malignant neoplasm. The cell of origin is considered to be the C-cell which is derived from the ultimobranchial body and neural crest. The tumor is solid and has characteristic amyloid in the stroma. The lesion usually presents as a neck mass, and multicentricity is not unusual. In some patients diarrhea may be an important presenting symptom. Serum calcitonin is typically elevated and is important in the diagnostic confirmation. About 10 percent of the cases are part of a familial endocrine syndrome which includes pheochromocytomas, mucosal neuromas, and other endocrine gland adenomas. Frequent multicentricity and early cervical lymphatic and systemic metastases necessitate total thyroidectomy and radical neck dissection. Prognosis varies from a rapid demise to long term survival.", "PMID": 966925}
{"id": "pubmed23n0032_6921", "title": "\"Practical suggestions on facial plastic surgery -- how I do it\". Augmentation mentoplasty using polyamide mesh.", "content": "Augmentation mentoplasty, a cosmetic surgical procedure to reshape the contour of the mental area, usually is accomplished with the insertion of autogenous graft or alloplastic implant material. Obtaining autogenous graft material has been the major disadvantage of requiring an additional surgical procedure. Alloplastic implant material is wiedly perferred because of its ready availability, ease of sterilization, capability of being cut, carved, and shaped as desired, and the relatively few postoperative complications that ensue from its use. The possible complications are infection, rejection by the host, displacement (even many years later), and underlying mandibular bone resorption. The author's four-year experience using polyamide mesh placed supraperiosteally in 100 patients is reported and the technique is described. Results have been consistently good, no implant has had to be removed or replaced. Other advantages are ease of inserting the polyamide mesh, thus facilitating the surgical procedure, minimal postoperative morbidity or reaction, flexibility in conforming exactly to the shape of the mandible, ingrowth of fibrous tissue thus avoiding late displacement, and providing a natural feel to the reconstructed area. There has been no roentgenographically demonstrable evidence that suggests bone resorption.", "contents": "\"Practical suggestions on facial plastic surgery -- how I do it\". Augmentation mentoplasty using polyamide mesh. Augmentation mentoplasty, a cosmetic surgical procedure to reshape the contour of the mental area, usually is accomplished with the insertion of autogenous graft or alloplastic implant material. Obtaining autogenous graft material has been the major disadvantage of requiring an additional surgical procedure. Alloplastic implant material is wiedly perferred because of its ready availability, ease of sterilization, capability of being cut, carved, and shaped as desired, and the relatively few postoperative complications that ensue from its use. The possible complications are infection, rejection by the host, displacement (even many years later), and underlying mandibular bone resorption. The author's four-year experience using polyamide mesh placed supraperiosteally in 100 patients is reported and the technique is described. Results have been consistently good, no implant has had to be removed or replaced. Other advantages are ease of inserting the polyamide mesh, thus facilitating the surgical procedure, minimal postoperative morbidity or reaction, flexibility in conforming exactly to the shape of the mandible, ingrowth of fibrous tissue thus avoiding late displacement, and providing a natural feel to the reconstructed area. There has been no roentgenographically demonstrable evidence that suggests bone resorption.", "PMID": 966926}
{"id": "pubmed23n0032_6922", "title": "[Operating room ethrane pollution (author's transl)].", "content": "Several surveys of ethrane pollution were made by the authors in operating rooms, either equipped or not with general ventilation systems, during operations where non-rebreathing anaesthesia devices were used. In operating rooms without general ventilation systems, but with simple air conditioning systems, ethrane pollution was found to be rather high and to attain 30-40 ppm at the fourth hour of operation. No statistically significant differences were elicited among levels found at the anaesthetist, surgeon and assistance personnel sites. The authors analyze the kinetics of ethrane pollution as a function of its consumption and of time and discuss the most suitable means for decreasig and preventing such pollution.", "contents": "[Operating room ethrane pollution (author's transl)]. Several surveys of ethrane pollution were made by the authors in operating rooms, either equipped or not with general ventilation systems, during operations where non-rebreathing anaesthesia devices were used. In operating rooms without general ventilation systems, but with simple air conditioning systems, ethrane pollution was found to be rather high and to attain 30-40 ppm at the fourth hour of operation. No statistically significant differences were elicited among levels found at the anaesthetist, surgeon and assistance personnel sites. The authors analyze the kinetics of ethrane pollution as a function of its consumption and of time and discuss the most suitable means for decreasig and preventing such pollution.", "PMID": 966927}
{"id": "pubmed23n0032_6923", "title": "[Benzene acute aleukemic leukosis, with widespread leukemic parenchymal infiltration (author's transl)].", "content": "A case is reported by the authors of a progressive benzene hemopathy with a final leukemic evolution. The leukemic pattern was an autopsy \"\"surprise'', inasmuch as never, even in the hours just before death, could leukemic cells be detected in the blood stream. The authors discuss some pathogenic aspects, with particular emphasis on the systemic spreading of a malignant hemopathy from very few leukemic cells in bone marrow.", "contents": "[Benzene acute aleukemic leukosis, with widespread leukemic parenchymal infiltration (author's transl)]. A case is reported by the authors of a progressive benzene hemopathy with a final leukemic evolution. The leukemic pattern was an autopsy \"\"surprise'', inasmuch as never, even in the hours just before death, could leukemic cells be detected in the blood stream. The authors discuss some pathogenic aspects, with particular emphasis on the systemic spreading of a malignant hemopathy from very few leukemic cells in bone marrow.", "PMID": 966928}
{"id": "pubmed23n0032_6924", "title": "[Epidemiologic evaluation of isocyanate immediate irritative action: 'one day effect' control (author's transl)].", "content": "Nineteen MDI exposed workers showed ventilatory changes, after eight hour exposure, mainly involving upper respiratory airways; the changes reversed following a bronchodilator aerosol. An incomplete recovery of VC was found in some subjects; therefore, the possibility of changes in the lung elastic recoil forces was hypothesized. No statistically significant differences were found as to occupation and smoking habit, which suggests that even low MDI concentrations could be enough to give rise to acute effects. The greater impairment found in subjects who were already affected by morning time emphasizes the necessity that such subjects were not employed in their specific work.", "contents": "[Epidemiologic evaluation of isocyanate immediate irritative action: 'one day effect' control (author's transl)]. Nineteen MDI exposed workers showed ventilatory changes, after eight hour exposure, mainly involving upper respiratory airways; the changes reversed following a bronchodilator aerosol. An incomplete recovery of VC was found in some subjects; therefore, the possibility of changes in the lung elastic recoil forces was hypothesized. No statistically significant differences were found as to occupation and smoking habit, which suggests that even low MDI concentrations could be enough to give rise to acute effects. The greater impairment found in subjects who were already affected by morning time emphasizes the necessity that such subjects were not employed in their specific work.", "PMID": 966929}
{"id": "pubmed23n0032_6925", "title": "[The medical management of carcinoma of the stomach (author's transl)].", "content": "Only a small proportion of patients with carcinoma of the stomach are suitable for radical surgery. For the remainder symptomatic treatment and radiotherapy alone or in combination with chemotherapy can be used to relieve distressing symptoms of pain, nausea and vomiting, and severe anemia. In the case of radiotherapy or chemotherapy, a degree of tumor regression can be expected. There have been encouraging results recently in palliating this disease with new radiotherapy techniques, notably fast neutron irradiation.", "contents": "[The medical management of carcinoma of the stomach (author's transl)]. Only a small proportion of patients with carcinoma of the stomach are suitable for radical surgery. For the remainder symptomatic treatment and radiotherapy alone or in combination with chemotherapy can be used to relieve distressing symptoms of pain, nausea and vomiting, and severe anemia. In the case of radiotherapy or chemotherapy, a degree of tumor regression can be expected. There have been encouraging results recently in palliating this disease with new radiotherapy techniques, notably fast neutron irradiation.", "PMID": 966930}
{"id": "pubmed23n0032_6926", "title": "[Surgical management of gastric carcinoma (author's transl)].", "content": "The treatment of choice of gastric carcinoma is still radical surgery which has to be tried in all cases which are not proven to be untreatable by this method. During surgery the tumor as well as regional lymph nodes and neighboring structures have to be removed; in special cases subtotal gastrectomy carrying a lower risk at surgery does allow complete removal of the tumor. Replacing the stomach by interposition of a long loop of the small intestine has proven to yield good results. The overall bad results of management of gastric carcinoma cannot be improved to any considerable extent by surgical means, e.g. by lowering the risk of surgery or by increasing the number of patients operated upon. Early diagnosis is of outmost importance since patients with tumors of an early stage have a rather good chance of being completely cured.", "contents": "[Surgical management of gastric carcinoma (author's transl)]. The treatment of choice of gastric carcinoma is still radical surgery which has to be tried in all cases which are not proven to be untreatable by this method. During surgery the tumor as well as regional lymph nodes and neighboring structures have to be removed; in special cases subtotal gastrectomy carrying a lower risk at surgery does allow complete removal of the tumor. Replacing the stomach by interposition of a long loop of the small intestine has proven to yield good results. The overall bad results of management of gastric carcinoma cannot be improved to any considerable extent by surgical means, e.g. by lowering the risk of surgery or by increasing the number of patients operated upon. Early diagnosis is of outmost importance since patients with tumors of an early stage have a rather good chance of being completely cured.", "PMID": 966931}
{"id": "pubmed23n0032_6927", "title": "[A new endoscopic method: the peroral choledocho-pancreatoscopy (author's transl)].", "content": "A new very thin glass fiber endoscope (\"baby scope\") has been developed, which can be passed through the channel of a modified duodenoscope (\"mother scope\") for catheterization of the choledochus or of the pancreatic duct. Peroral choledocho-pancreatoscopy (PCPS) was performed in 9 patients, the endoscopic results are presented. Other new auxiliary instruments for diagnostic or therapeutic purposes in biliary or pancreatic diseases are described: a brushing catheter, a special biopsy forceps, a retention probe and a balloon-tipped catheter.", "contents": "[A new endoscopic method: the peroral choledocho-pancreatoscopy (author's transl)]. A new very thin glass fiber endoscope (\"baby scope\") has been developed, which can be passed through the channel of a modified duodenoscope (\"mother scope\") for catheterization of the choledochus or of the pancreatic duct. Peroral choledocho-pancreatoscopy (PCPS) was performed in 9 patients, the endoscopic results are presented. Other new auxiliary instruments for diagnostic or therapeutic purposes in biliary or pancreatic diseases are described: a brushing catheter, a special biopsy forceps, a retention probe and a balloon-tipped catheter.", "PMID": 966932}
{"id": "pubmed23n0032_6928", "title": "[Endoscopy of the upper gastrointestinal tract with the new panendoscope f7 (author's transl)].", "content": "The Panendoscope F7 with a diameter of 9.5 mm, originally designed for pediatric endoscopy, was used in adults. First results are reported. The instrument has a shorter flexible tip facilitating biopsies in the esophagus. During exploration of the duodenal bulb tip of the instrument can be inverted in most cases allowing inspection of the pyloric region of the duodenum. The aboral part of a gastrojejunal anastomosis after partial gastrectomy can be visualized with the inverted tip of the panendoscope as well. The instrument does also facilitate endoscopic examination of the remaining stomach and of the afferent loop in patients partially gastrectomized. Emergency endoscopy can be done while a gastric tube removing blood during the examination may stay in position.", "contents": "[Endoscopy of the upper gastrointestinal tract with the new panendoscope f7 (author's transl)]. The Panendoscope F7 with a diameter of 9.5 mm, originally designed for pediatric endoscopy, was used in adults. First results are reported. The instrument has a shorter flexible tip facilitating biopsies in the esophagus. During exploration of the duodenal bulb tip of the instrument can be inverted in most cases allowing inspection of the pyloric region of the duodenum. The aboral part of a gastrojejunal anastomosis after partial gastrectomy can be visualized with the inverted tip of the panendoscope as well. The instrument does also facilitate endoscopic examination of the remaining stomach and of the afferent loop in patients partially gastrectomized. Emergency endoscopy can be done while a gastric tube removing blood during the examination may stay in position.", "PMID": 966933}
{"id": "pubmed23n0032_6929", "title": "[Lymphangiosis carcinomatosa of the lung and cardiopulmonary shock as initial findings in gastric cancer (author's transl)].", "content": "A case is reported of a 34 year old woman, who was hospitalized because of cardiopulmonary shock of sudden unsuspected onset. X-ray examination revealed diffuse interstitial pulmonary infiltration. Intra-aortal counter-pulsation did improve the condition only for short time. On autopsy an adenocarcinoma of the stomach was found, as well as diffuse carcinomatous infiltration of pulmonary lymph and arterial vessels. Thus lymphangiosis carcinomatosa has to be taken into consideration in discussing the differential diagnosis of diffuse interstitial pulmonary infiltration in young patients. The presence of microangiopathic hemolytic anemia may help to establish the diagnosis.", "contents": "[Lymphangiosis carcinomatosa of the lung and cardiopulmonary shock as initial findings in gastric cancer (author's transl)]. A case is reported of a 34 year old woman, who was hospitalized because of cardiopulmonary shock of sudden unsuspected onset. X-ray examination revealed diffuse interstitial pulmonary infiltration. Intra-aortal counter-pulsation did improve the condition only for short time. On autopsy an adenocarcinoma of the stomach was found, as well as diffuse carcinomatous infiltration of pulmonary lymph and arterial vessels. Thus lymphangiosis carcinomatosa has to be taken into consideration in discussing the differential diagnosis of diffuse interstitial pulmonary infiltration in young patients. The presence of microangiopathic hemolytic anemia may help to establish the diagnosis.", "PMID": 966934}
{"id": "pubmed23n0032_6930", "title": "[The pathogenesis of gastric carcinoma--epidemiologic pathology of precursor lesions (author's transl)].", "content": "An overview of the epidemiologic and pathologic significance of premalignant lesions of the stomach is presented, with correlations between cancer risk, prevalence of atrophic gastritis and environmental factors such as nitrate and food items. The protective role of lettuce and fresh vegetables is discussed. The diffuse type of gastric cancer appears to be less environmentally linked than the intestinal type, and it is hypothesized that the pathogenetic process may begin during the first decade of life. An analysis of precursor lesions and gastric cancer in various geographic regions of Columbia, South America is presented to illustrate these relationships.", "contents": "[The pathogenesis of gastric carcinoma--epidemiologic pathology of precursor lesions (author's transl)]. An overview of the epidemiologic and pathologic significance of premalignant lesions of the stomach is presented, with correlations between cancer risk, prevalence of atrophic gastritis and environmental factors such as nitrate and food items. The protective role of lettuce and fresh vegetables is discussed. The diffuse type of gastric cancer appears to be less environmentally linked than the intestinal type, and it is hypothesized that the pathogenetic process may begin during the first decade of life. An analysis of precursor lesions and gastric cancer in various geographic regions of Columbia, South America is presented to illustrate these relationships.", "PMID": 966935}
{"id": "pubmed23n0032_6931", "title": "[Experimental gastric cancer (author's transl)].", "content": "Methods have been established to produce gastric cancer in rats and dogs by administration of N-methyl-N'-nitro-N-nitrosoguanidine or of the ethyl derivate. The agent is administered in drinking water or by a pellet diet soaked in the carcinogen. Histologically well differentiated and poorly differentiated types of adenocarcinoma and signet-ring cell tumors are induced in several months with greath reliability. Metastases were observed in both rats and dogs with gastric carcinoma. The carcinogenic effect could be enhanced by surface active agents, sodium chloride, iodoacetamide, insertion of plastic beads into the stomach and gastroenteroanastomosis. Follow-up studies by radiologic, endoscopic and bioptic examinations are possible in the dog. There are similarities in these experimental tumors to those in man and thus they provide means for the investigation of histogenesis, prevention, and chemotherapy of gastric cancer. An adenocarcinoma of the glandular stomach of a Wistar rat was successively transplanted to new born rats of the same strain.", "contents": "[Experimental gastric cancer (author's transl)]. Methods have been established to produce gastric cancer in rats and dogs by administration of N-methyl-N'-nitro-N-nitrosoguanidine or of the ethyl derivate. The agent is administered in drinking water or by a pellet diet soaked in the carcinogen. Histologically well differentiated and poorly differentiated types of adenocarcinoma and signet-ring cell tumors are induced in several months with greath reliability. Metastases were observed in both rats and dogs with gastric carcinoma. The carcinogenic effect could be enhanced by surface active agents, sodium chloride, iodoacetamide, insertion of plastic beads into the stomach and gastroenteroanastomosis. Follow-up studies by radiologic, endoscopic and bioptic examinations are possible in the dog. There are similarities in these experimental tumors to those in man and thus they provide means for the investigation of histogenesis, prevention, and chemotherapy of gastric cancer. An adenocarcinoma of the glandular stomach of a Wistar rat was successively transplanted to new born rats of the same strain.", "PMID": 966936}
{"id": "pubmed23n0032_6932", "title": "[Gastric cancer--an attempt to correlate experimental and clinical findings (author's transl)].", "content": "The correlation of induced gastric cancer in experimental animals with spontaneous gastric cancer in man may be based on several parameters. Malignant tumors have many properties, such as growth, heterotopia, anaplasia, unresponsiveness to extrinsic stimuli, and ability to evoke local and systemic reactions in the autologous host. Individually these properties do not characterise malignancy, but collectively they provide criteria for correlation of human and experimental gastric cancer. Accumulated data, particularly recent findings, attest to many similarities of induced animal and spontaneous human gastric cancer. However, the complexity of the disease is emphasized and data indicate a multi-causative etiology, with various factors interacting in a multi-coordinate manner.", "contents": "[Gastric cancer--an attempt to correlate experimental and clinical findings (author's transl)]. The correlation of induced gastric cancer in experimental animals with spontaneous gastric cancer in man may be based on several parameters. Malignant tumors have many properties, such as growth, heterotopia, anaplasia, unresponsiveness to extrinsic stimuli, and ability to evoke local and systemic reactions in the autologous host. Individually these properties do not characterise malignancy, but collectively they provide criteria for correlation of human and experimental gastric cancer. Accumulated data, particularly recent findings, attest to many similarities of induced animal and spontaneous human gastric cancer. However, the complexity of the disease is emphasized and data indicate a multi-causative etiology, with various factors interacting in a multi-coordinate manner.", "PMID": 966937}
{"id": "pubmed23n0032_6933", "title": "Effects of beta-hydroxybutyrate, glycerol, and free fatty acid infusions on glucagon and epinephrine secretion in dogs during acute hypoglycemia.", "content": "The importance of glucagon in the regulation of carbohydrate metabolism is clearly established. However, the role played by this hormone in the regulation of the overall fuel economy is less certain, particularly with respect to such nonglucose fuels as free fatty acids, glycerol, and ketoacids. In order to elucidate glucagon's role with respect to the latter substrates, dogs were infused with solutions of these three fuels, and their A-cell responses to concomitant insulin-induced hypoglycemia were studied. In addition, epinephrine levels were also monitored. It was found that while these infusions failed to suppress glucagon release, the ketoacid infusion did significantly reduce epinephrine secretion during the insulin-induced hypoglycemic period. It was therefore concluded that glucagon secretion under these experimental conditions is not responsive to prevailing non-glucose fuel levels. Indeed, these data suggest that the sympathetic nervous system may play an important role in the regulation of the over-all fuel economy.", "contents": "Effects of beta-hydroxybutyrate, glycerol, and free fatty acid infusions on glucagon and epinephrine secretion in dogs during acute hypoglycemia. The importance of glucagon in the regulation of carbohydrate metabolism is clearly established. However, the role played by this hormone in the regulation of the overall fuel economy is less certain, particularly with respect to such nonglucose fuels as free fatty acids, glycerol, and ketoacids. In order to elucidate glucagon's role with respect to the latter substrates, dogs were infused with solutions of these three fuels, and their A-cell responses to concomitant insulin-induced hypoglycemia were studied. In addition, epinephrine levels were also monitored. It was found that while these infusions failed to suppress glucagon release, the ketoacid infusion did significantly reduce epinephrine secretion during the insulin-induced hypoglycemic period. It was therefore concluded that glucagon secretion under these experimental conditions is not responsive to prevailing non-glucose fuel levels. Indeed, these data suggest that the sympathetic nervous system may play an important role in the regulation of the over-all fuel economy.", "PMID": 967013}
{"id": "pubmed23n0032_6934", "title": "Alterations in proline metabolic enzymes with mammalian development.", "content": "Through the use of specific radioisotopic assays, the activities of enzymes degrading and synthesizing proline were examined in rat liver and kidney as a function of development. Proline oxidase (PO), the enzyme converting proline to delta 1-pyrroline-5-carboxylate (PC), undergoes 15- and eight-fold increases in liver and kidney, respectively, as rats mature from term-fetal to adult life (6-12 wk). The differences are not due to enzyme inhibitors or activators, and kinetic analysis reveals the change to be one of greater tissue content of the same enzyme. delta 1-Pyrroline-5-carboxylate dehydrogenase, which converts PC to glutamate, shows a two- to three-fold increase in both tissues, paralleling the changes in PO with development. delta 1-Pyrroline-5-carboxylate reductase (PCR), the enzyme which catalyzes the committed step in endogenous proline formation, undergoes oppositely directed changes, such that adult levels are only 20%-25% of fetal levels in liver and kidney. PO/PCR ratios are 25- to 50-fold greater in adult central tissues than they are in fetal tissues. Thus, the central tissues of adult rats appear to function as proline utilizers, whereas those of young rats are chiefly proline formers. This difference may relate to different rates of utilization of proline for protein synthesis in young and adult rats.", "contents": "Alterations in proline metabolic enzymes with mammalian development. Through the use of specific radioisotopic assays, the activities of enzymes degrading and synthesizing proline were examined in rat liver and kidney as a function of development. Proline oxidase (PO), the enzyme converting proline to delta 1-pyrroline-5-carboxylate (PC), undergoes 15- and eight-fold increases in liver and kidney, respectively, as rats mature from term-fetal to adult life (6-12 wk). The differences are not due to enzyme inhibitors or activators, and kinetic analysis reveals the change to be one of greater tissue content of the same enzyme. delta 1-Pyrroline-5-carboxylate dehydrogenase, which converts PC to glutamate, shows a two- to three-fold increase in both tissues, paralleling the changes in PO with development. delta 1-Pyrroline-5-carboxylate reductase (PCR), the enzyme which catalyzes the committed step in endogenous proline formation, undergoes oppositely directed changes, such that adult levels are only 20%-25% of fetal levels in liver and kidney. PO/PCR ratios are 25- to 50-fold greater in adult central tissues than they are in fetal tissues. Thus, the central tissues of adult rats appear to function as proline utilizers, whereas those of young rats are chiefly proline formers. This difference may relate to different rates of utilization of proline for protein synthesis in young and adult rats.", "PMID": 967014}
{"id": "pubmed23n0032_6935", "title": "Insulin sensitivity of adipose tissue in vitro and the response to exogenous insulin in obese human subjects.", "content": "The effect of varying concentrations of insulin on 1-14C-glucose conversion to 14CO2 was measured in subcutaneous adipose tissue samples obtained from 16 obese human subjects (10 nondiabetic, 6 diabetic). An index of insulin sensitivity in vitro, Kins, was calculated as the concentration of insulin stimulating one-half maximal 14CO2 production. An index of insulin sensitivity in vivo, Kitt, was calculated as the rate constant for decrease in blood glucose after rapid intravenous administration of 0.05 U/kg insulin. There was, over-all, a significant correlation between Kins and Kitt, indicating that insulin sensitivity of 1-14C-glucose oxidation by adipose tissue in vitro reflects the general state of sensitivity of glucose metabolism to insulin in vivo in obese human subjects. The mean values for both Kins and Kitt in the nondiabetic subjects were significantly different from those in the diabetic subjects, indicating greater sensitivity to insulin in the former group. The nondiabetic group was also distinguished by a significantly greater plasma insulin:blood glucose ratio in the oral glucose tolerance test. These results support the view that tissue insulin sensitivity as well as pancreatic beta cell response play an important role in determining glucose tolerance in obesity.", "contents": "Insulin sensitivity of adipose tissue in vitro and the response to exogenous insulin in obese human subjects. The effect of varying concentrations of insulin on 1-14C-glucose conversion to 14CO2 was measured in subcutaneous adipose tissue samples obtained from 16 obese human subjects (10 nondiabetic, 6 diabetic). An index of insulin sensitivity in vitro, Kins, was calculated as the concentration of insulin stimulating one-half maximal 14CO2 production. An index of insulin sensitivity in vivo, Kitt, was calculated as the rate constant for decrease in blood glucose after rapid intravenous administration of 0.05 U/kg insulin. There was, over-all, a significant correlation between Kins and Kitt, indicating that insulin sensitivity of 1-14C-glucose oxidation by adipose tissue in vitro reflects the general state of sensitivity of glucose metabolism to insulin in vivo in obese human subjects. The mean values for both Kins and Kitt in the nondiabetic subjects were significantly different from those in the diabetic subjects, indicating greater sensitivity to insulin in the former group. The nondiabetic group was also distinguished by a significantly greater plasma insulin:blood glucose ratio in the oral glucose tolerance test. These results support the view that tissue insulin sensitivity as well as pancreatic beta cell response play an important role in determining glucose tolerance in obesity.", "PMID": 967015}
{"id": "pubmed23n0032_6936", "title": "Metabolic responses to dietary supplements of bran.", "content": "During a metabolic ward study, the addition of dietary fiber in the form of wheat bran biscuits to the diet of five volunteer subjects resulted in an increase in the stool wet weight and fecal solids. The excretion of fecal solids was highly correlated with the intake of unavailable carbohydrates, and fecal losses of water were similarly correlated with fecal excretion of these constituents. The major component of the increase in fecal solids was due to the noncellulosic polysaccharide fraction of dietary fiber. There was an increased fecal excretion of nitrogen fat and energy by most subjects when the supplement was eaten. However, the increased loss of energy in the feces was only 40-80 kcal/day, and therefore a large supplemental intake of dietary fiber had only minor effects on energy metabolism. Supplemental fiber is thus unlikely to induce a useful loss of calories in the management of obesity. The addition of dietary fiber caused an increased excretion of most inorganic constituents, particularly sodium and phosphorus; increased excretion of iron and magnesium was also found in two subjects.", "contents": "Metabolic responses to dietary supplements of bran. During a metabolic ward study, the addition of dietary fiber in the form of wheat bran biscuits to the diet of five volunteer subjects resulted in an increase in the stool wet weight and fecal solids. The excretion of fecal solids was highly correlated with the intake of unavailable carbohydrates, and fecal losses of water were similarly correlated with fecal excretion of these constituents. The major component of the increase in fecal solids was due to the noncellulosic polysaccharide fraction of dietary fiber. There was an increased fecal excretion of nitrogen fat and energy by most subjects when the supplement was eaten. However, the increased loss of energy in the feces was only 40-80 kcal/day, and therefore a large supplemental intake of dietary fiber had only minor effects on energy metabolism. Supplemental fiber is thus unlikely to induce a useful loss of calories in the management of obesity. The addition of dietary fiber caused an increased excretion of most inorganic constituents, particularly sodium and phosphorus; increased excretion of iron and magnesium was also found in two subjects.", "PMID": 967016}
{"id": "pubmed23n0032_6937", "title": "The importance of the stomach in mediating histamine-induced hypocalcemia in the rat.", "content": "The effect of histamine on serum calcium homeostasis was studied in the rat. After the intravenous administration of 0.5-1.0 mg histamine base to fasted Holtzman rats weighing 80-100 g, a significant lowering of serum calcium (Ca) level occurred 30 min after injection (decrease in Ca, 1.4-1.9 mg/100 ml), but normocalcemia returned at 60 min. Repeat intravenous injections of histamine 1.0 mg resulted in repeated lowering of the serum Ca level. Hypophosphatemia did not accompany the hypocalcemia. Thyroparathyroidectomy (TPTX) did not eliminate the calcium-lowering effect of histamine in acute TPTX animals but did so in more chronic TPTX animals in which the mean serum Ca was 7.6 mg/100 ml or less. Gastrectomy, however, completely eliminated the calcium-lowering effect of histamine given in doses of up to 2 mg/rat (20 mg/kg of body weight), despite the presence of an intact thyroid gland. These studies support the role of a gastric factor and not the thyroid secretion of calcitonin in mediating this response in the rat.", "contents": "The importance of the stomach in mediating histamine-induced hypocalcemia in the rat. The effect of histamine on serum calcium homeostasis was studied in the rat. After the intravenous administration of 0.5-1.0 mg histamine base to fasted Holtzman rats weighing 80-100 g, a significant lowering of serum calcium (Ca) level occurred 30 min after injection (decrease in Ca, 1.4-1.9 mg/100 ml), but normocalcemia returned at 60 min. Repeat intravenous injections of histamine 1.0 mg resulted in repeated lowering of the serum Ca level. Hypophosphatemia did not accompany the hypocalcemia. Thyroparathyroidectomy (TPTX) did not eliminate the calcium-lowering effect of histamine in acute TPTX animals but did so in more chronic TPTX animals in which the mean serum Ca was 7.6 mg/100 ml or less. Gastrectomy, however, completely eliminated the calcium-lowering effect of histamine given in doses of up to 2 mg/rat (20 mg/kg of body weight), despite the presence of an intact thyroid gland. These studies support the role of a gastric factor and not the thyroid secretion of calcitonin in mediating this response in the rat.", "PMID": 967017}
{"id": "pubmed23n0032_6938", "title": "[Biological and medical applications of the cathodoluminescence in the scanning electron microscope (author's transl)].", "content": "The light emission according to the excitation of solids and molecules by electrons--named cathodoluminescence--extends the analytical field of applications of the scanning electron microscope. This method can be applied in biological and medical research. It can be used for the investigation of marked specimens similar to the fluorescence microscopy. The autoluminescence of some substances can also be used for special applications. The cathodoluminescence in the scanning electron microscope has the advantage that more sensitive detector-systems can be used than with the fluorescence microscope. Moreover, in the scanning microscope it is possible to combine the cathodoluminescence mode with other imaging or analytical equipments.", "contents": "[Biological and medical applications of the cathodoluminescence in the scanning electron microscope (author's transl)]. The light emission according to the excitation of solids and molecules by electrons--named cathodoluminescence--extends the analytical field of applications of the scanning electron microscope. This method can be applied in biological and medical research. It can be used for the investigation of marked specimens similar to the fluorescence microscopy. The autoluminescence of some substances can also be used for special applications. The cathodoluminescence in the scanning electron microscope has the advantage that more sensitive detector-systems can be used than with the fluorescence microscope. Moreover, in the scanning microscope it is possible to combine the cathodoluminescence mode with other imaging or analytical equipments.", "PMID": 967018}
{"id": "pubmed23n0032_6939", "title": "[A coordinate and area recording microscope for the topographic analysis of particle size distributions (author's transl)].", "content": "A coordinate recording microscope equipped with a rolling disc planimeter is described. The application of the instrument for the analysis of cell size distribution in histologic preparations is demonstrated. The microscope stage is moved in x, and y directions by two digital micrometer spindles. The planimeter is equipped with a rotary encoder. The spindles and the rotary encoder are connected to digital counters which are input to a multiplexer. The multiplexer outputs the data to a teletype terminal both as hard copy and on paper tape. To avoid repeated measurements a video-acoustic monitoring system is provided. Processing of the data is carried out off-line with a IBM 1130 computer. Here the measured cells are displayed as points or circles on an oscilloscope. The distribution of cells of any size can be studied topographically in any region of the field of measurements.", "contents": "[A coordinate and area recording microscope for the topographic analysis of particle size distributions (author's transl)]. A coordinate recording microscope equipped with a rolling disc planimeter is described. The application of the instrument for the analysis of cell size distribution in histologic preparations is demonstrated. The microscope stage is moved in x, and y directions by two digital micrometer spindles. The planimeter is equipped with a rotary encoder. The spindles and the rotary encoder are connected to digital counters which are input to a multiplexer. The multiplexer outputs the data to a teletype terminal both as hard copy and on paper tape. To avoid repeated measurements a video-acoustic monitoring system is provided. Processing of the data is carried out off-line with a IBM 1130 computer. Here the measured cells are displayed as points or circles on an oscilloscope. The distribution of cells of any size can be studied topographically in any region of the field of measurements.", "PMID": 967019}
{"id": "pubmed23n0032_6940", "title": "Incoherent bar images formed by a polarizing microscope with crossed polarizers.", "content": "Diffraction images of incoherent bar objects formed by a polarizing microscope with crossed polarizers are studied. Expression for intensity distribution has been derived and the results in the form of curves have been plotted. The images which are of unusual shape, different from the patterns formed by the ordinary microscope are strongly dependent upon the size of the bar/coherence parameter. The line spread function can be obtained by taking a very narrow bar.", "contents": "Incoherent bar images formed by a polarizing microscope with crossed polarizers. Diffraction images of incoherent bar objects formed by a polarizing microscope with crossed polarizers are studied. Expression for intensity distribution has been derived and the results in the form of curves have been plotted. The images which are of unusual shape, different from the patterns formed by the ordinary microscope are strongly dependent upon the size of the bar/coherence parameter. The line spread function can be obtained by taking a very narrow bar.", "PMID": 967020}
{"id": "pubmed23n0032_6941", "title": "Dynamics of myocardial infarction--the role of critical closing pressure.", "content": "Pressure in the primary distributing network of a vessel on the surface of the heart is normally sufficient to prevent critical closure of its derivative vessels. It is proposed that acute occlusion of a major supply vessel or over activity of the heart in the presence of an impairment of the lumen of a major supply vessel may reduce transmural pressure in parts of the network. In consequence vasospasm, the result directly of critical closure, is postulated as the basis for myocardial infarction, together with maximal dilatation of vessels in which pressure did not fall to critical levels.", "contents": "Dynamics of myocardial infarction--the role of critical closing pressure. Pressure in the primary distributing network of a vessel on the surface of the heart is normally sufficient to prevent critical closure of its derivative vessels. It is proposed that acute occlusion of a major supply vessel or over activity of the heart in the presence of an impairment of the lumen of a major supply vessel may reduce transmural pressure in parts of the network. In consequence vasospasm, the result directly of critical closure, is postulated as the basis for myocardial infarction, together with maximal dilatation of vessels in which pressure did not fall to critical levels.", "PMID": 967062}
{"id": "pubmed23n0032_6942", "title": "Ascorbic acid and diabetes mellitus.", "content": "Dehydroascorbic acid, the oxidized form of vitamin C, causes diabetes when injected into animals and has been reported to be present in increased amounts in the blood of patients with diabetes mellitus and even in prediabetics. One of the earliest changes in diabetes mellitus is electron-microscopic evidence of damage to the inner endothelial lining of the blood vessels. Certain bioflavonoids, which are natural non-toxic food substances from plants, like rutin from buckwheat, prevent the oxidation of ascorbic acid and seem to protect the endothelium when given with vitamin C; it is therefore suggested that all vitamin C tablets should be combined with these flavonoids.", "contents": "Ascorbic acid and diabetes mellitus. Dehydroascorbic acid, the oxidized form of vitamin C, causes diabetes when injected into animals and has been reported to be present in increased amounts in the blood of patients with diabetes mellitus and even in prediabetics. One of the earliest changes in diabetes mellitus is electron-microscopic evidence of damage to the inner endothelial lining of the blood vessels. Certain bioflavonoids, which are natural non-toxic food substances from plants, like rutin from buckwheat, prevent the oxidation of ascorbic acid and seem to protect the endothelium when given with vitamin C; it is therefore suggested that all vitamin C tablets should be combined with these flavonoids.", "PMID": 967063}
{"id": "pubmed23n0032_6943", "title": "On the potential usefulness of exchange plasmapheresis in the immunotherapy of cancer and of some chronic persistent infections.", "content": "In an individual sensitized to an antigen, Withdrawal of the corresponding antibody and of the accompanying antigen-antibody complexes stimulates antibody production: the end result is thus not unlike the effect of a booster dose of that antigen. Conversely, a sufficient concentration of antigen-antibody complexes will eventually shut off the antibody production to that antigen. The body is able to regulate through this mechanism the amplitude of the immune response, using the feed-back interaction of antigen-antibody complexes with the immune system. Without such a control system any antigenic stimulation would result in an uncontrolled out-pouring of antibodies, as is observed in myeloma. The regulation of cell mediated immunity is also indirectly affected by the concentration of circulating antigen-antibody complexes. Other mechanisms of immunoregulation are also at work, using the mediation of so-called suppressor cells, identified as sub-populations of T and B cells acting both specifically and non-specifically on immune effectors cells. It is likely that a major factor contributing to the pathogenesis of some persistent chronic infections such as syphilis, brucellosis, chronic viral hepatitis, leprosy, vaccinia, congenital cytomegalovirus infection persisting in childhood, and so on, and in conditions such as cancer, is an inadequate initial production of antibodies, further aggravated by the ensuing immunosuppression brought about by the formation of antigen-antibody complexes. Antigen-antibody complexes have indeed been identified as playing a prominent role in some of these diseases. It is also suggested that the magnitude of the initial antigenic dose may influence the ensuing immune response: while a large antigen dose could induce a \"classic\" and efficient immune response, a low antigen dose, such as an incipient neoplasm, could result in a minimal antibody response, further suppressed by the appearance of antigen-antibody complexes. Through this mechanism, a premature failure to eradicate the disease would follow. I suggest that a significant and sudden lowering of the concentration of relevant entibodies and antigen-antibody complexes through exchange plasmapheresis, would trigger a fully adequate and therapeutic immune response. This possibility is discussed.", "contents": "On the potential usefulness of exchange plasmapheresis in the immunotherapy of cancer and of some chronic persistent infections. In an individual sensitized to an antigen, Withdrawal of the corresponding antibody and of the accompanying antigen-antibody complexes stimulates antibody production: the end result is thus not unlike the effect of a booster dose of that antigen. Conversely, a sufficient concentration of antigen-antibody complexes will eventually shut off the antibody production to that antigen. The body is able to regulate through this mechanism the amplitude of the immune response, using the feed-back interaction of antigen-antibody complexes with the immune system. Without such a control system any antigenic stimulation would result in an uncontrolled out-pouring of antibodies, as is observed in myeloma. The regulation of cell mediated immunity is also indirectly affected by the concentration of circulating antigen-antibody complexes. Other mechanisms of immunoregulation are also at work, using the mediation of so-called suppressor cells, identified as sub-populations of T and B cells acting both specifically and non-specifically on immune effectors cells. It is likely that a major factor contributing to the pathogenesis of some persistent chronic infections such as syphilis, brucellosis, chronic viral hepatitis, leprosy, vaccinia, congenital cytomegalovirus infection persisting in childhood, and so on, and in conditions such as cancer, is an inadequate initial production of antibodies, further aggravated by the ensuing immunosuppression brought about by the formation of antigen-antibody complexes. Antigen-antibody complexes have indeed been identified as playing a prominent role in some of these diseases. It is also suggested that the magnitude of the initial antigenic dose may influence the ensuing immune response: while a large antigen dose could induce a \"classic\" and efficient immune response, a low antigen dose, such as an incipient neoplasm, could result in a minimal antibody response, further suppressed by the appearance of antigen-antibody complexes. Through this mechanism, a premature failure to eradicate the disease would follow. I suggest that a significant and sudden lowering of the concentration of relevant entibodies and antigen-antibody complexes through exchange plasmapheresis, would trigger a fully adequate and therapeutic immune response. This possibility is discussed.", "PMID": 967065}
{"id": "pubmed23n0032_6944", "title": "On the evolution of X-chromosome inactivation in mammals and the clinical consequences to man--a hypothesis.", "content": "A clinical analysis of abnormal sex chromosome states in man suggests that Lyon's recent X-Y translocation hypothesis for the evolution of X-chromosome inactivation in mammals most likely would have lead to an evolutionary dead-end. Therefore, as an alternate I have hypothesized that: X-chromosome inactivation in somatic cells of mammals could have evolved by a complementary process of one by one heterozygous physical deletion in males and heterozygous inactivation in females of genes for \"somatic\" traits scattered throughout the genome whose effective output had become 50% excessive during prior evolution. However, this complementary process could occur safely only if the genes so deleted or inactivated first segregated by chance onto the evolving sex-chromosomes via a one by one reciprocal exchange for non-sex related genes already there. The complementary process thereby would allow slow evolution of the Y-chromosome in the male and X-chromosome inactivation in the female. Evolution of X-chromosome inactivation in this manner is compatible with Ohno's observation of \"conservation\" of the X-chromosome in mammals; and the occurrance of clinical \"somatic\" abnormalities in the abnormal X or Y chromosome states of man despite X-chromosome inactivation.", "contents": "On the evolution of X-chromosome inactivation in mammals and the clinical consequences to man--a hypothesis. A clinical analysis of abnormal sex chromosome states in man suggests that Lyon's recent X-Y translocation hypothesis for the evolution of X-chromosome inactivation in mammals most likely would have lead to an evolutionary dead-end. Therefore, as an alternate I have hypothesized that: X-chromosome inactivation in somatic cells of mammals could have evolved by a complementary process of one by one heterozygous physical deletion in males and heterozygous inactivation in females of genes for \"somatic\" traits scattered throughout the genome whose effective output had become 50% excessive during prior evolution. However, this complementary process could occur safely only if the genes so deleted or inactivated first segregated by chance onto the evolving sex-chromosomes via a one by one reciprocal exchange for non-sex related genes already there. The complementary process thereby would allow slow evolution of the Y-chromosome in the male and X-chromosome inactivation in the female. Evolution of X-chromosome inactivation in this manner is compatible with Ohno's observation of \"conservation\" of the X-chromosome in mammals; and the occurrance of clinical \"somatic\" abnormalities in the abnormal X or Y chromosome states of man despite X-chromosome inactivation.", "PMID": 967064}
{"id": "pubmed23n0032_6945", "title": "\"Basal\" palmar skin potentials and body fluid potassium.", "content": "When palmar eccrine sweat glands are inactive the potential difference between palmar skin and a prepared forearm site is a function of the ratio of external (electrode electrolyte) and internal (tissue fluid) potassium concentrations. Evidence indicates that this \"basal\" palmar skin potential changes systematically with changes in ECF K+, and may be used to monitor such shifts, as, for example, in stress.", "contents": "\"Basal\" palmar skin potentials and body fluid potassium. When palmar eccrine sweat glands are inactive the potential difference between palmar skin and a prepared forearm site is a function of the ratio of external (electrode electrolyte) and internal (tissue fluid) potassium concentrations. Evidence indicates that this \"basal\" palmar skin potential changes systematically with changes in ECF K+, and may be used to monitor such shifts, as, for example, in stress.", "PMID": 967067}
{"id": "pubmed23n0032_6946", "title": "Computer analysis of external counterpulsation by use of a nonlinear mathematical model of the cardiovascular system.", "content": "A pressure system externally applied to the limbs of a patient has been proven effective in assisting circulation in animal and clinical studies. In this study a nonlinear mathematical model of the cardiovascular system is utilized to determine the effectiveness of high-frequency components in the external pressure waveform. The development of the model, the method of analysis, and the results acquired with the model are presented in this report, demonstrating its suitability for the study of external counterpulsation.", "contents": "Computer analysis of external counterpulsation by use of a nonlinear mathematical model of the cardiovascular system. A pressure system externally applied to the limbs of a patient has been proven effective in assisting circulation in animal and clinical studies. In this study a nonlinear mathematical model of the cardiovascular system is utilized to determine the effectiveness of high-frequency components in the external pressure waveform. The development of the model, the method of analysis, and the results acquired with the model are presented in this report, demonstrating its suitability for the study of external counterpulsation.", "PMID": 967070}
{"id": "pubmed23n0032_6947", "title": "Counterpulsation with a new pulsatile assist device (PAD) in open-heart surgery.", "content": "A new pulsatile assist device that converts roller pump flow to pulsatile flow has been developed and proven effective through clinical testing. The PAD is also a hemodynamically effective arterial counterpulsator both before and after cardiopulmonary bypass. The PAD has been found to be an important adjunct to the care of critically ill patients undergoing open-heart surgery. In addition, in selected patients, the PAD is a reasonable alternative to the elective use of intra-aortic balloon pumping.", "contents": "Counterpulsation with a new pulsatile assist device (PAD) in open-heart surgery. A new pulsatile assist device that converts roller pump flow to pulsatile flow has been developed and proven effective through clinical testing. The PAD is also a hemodynamically effective arterial counterpulsator both before and after cardiopulmonary bypass. The PAD has been found to be an important adjunct to the care of critically ill patients undergoing open-heart surgery. In addition, in selected patients, the PAD is a reasonable alternative to the elective use of intra-aortic balloon pumping.", "PMID": 967071}
{"id": "pubmed23n0032_6948", "title": "Cortisol in physiological concentrations acts within minutes to modify effects of prolactin and growth hormone on prostaglandin secretion: importance of effect in modulating cellular responses to calcium and cyclic nucleotides.", "content": "We propose that intracellular prostaglandins (PGs) are essential for the final expression of the effects of second messengers in most cells. We suggest that the amounts of PGs required are very small (in the picomolar range) and are much lower than those used in most current PG studies. We suggest that while therapeutic levels of inhibitors of PG synthetase may be adequate to block the overflow of PGs from cells, they are in most cases unlikely to reduce intracellular PGs sufficiently to test the role of such PGs. We propose that there is a basal level of PG synthesis unaffected by hormones but that above this level PG synthesis is regulated by the interplay between physiological levels of cortisol, prolactin, growth hormone and thyroid hormones. For the most part prolactin seems to stimulate PG synthesis and cortisol to inhibit it: cortisol has, however, no inhibitory effect on basal PG synthesis. In reducing prolactin-stimulated PG synthesis cortisol is 1000-2000 times more potent than indomethacin on a molar basis. We suggest that the regulation of intracellular PG levels is the mechanism of the so-called \"permissive\" actions of these hormones. These concepts could prove important in the understanding of many aspects of physiology and pathophysiology including diurnal and seasonal changes in hormone responsiveness. They are also relevant to the use of established drugs and the design of new ones.", "contents": "Cortisol in physiological concentrations acts within minutes to modify effects of prolactin and growth hormone on prostaglandin secretion: importance of effect in modulating cellular responses to calcium and cyclic nucleotides. We propose that intracellular prostaglandins (PGs) are essential for the final expression of the effects of second messengers in most cells. We suggest that the amounts of PGs required are very small (in the picomolar range) and are much lower than those used in most current PG studies. We suggest that while therapeutic levels of inhibitors of PG synthetase may be adequate to block the overflow of PGs from cells, they are in most cases unlikely to reduce intracellular PGs sufficiently to test the role of such PGs. We propose that there is a basal level of PG synthesis unaffected by hormones but that above this level PG synthesis is regulated by the interplay between physiological levels of cortisol, prolactin, growth hormone and thyroid hormones. For the most part prolactin seems to stimulate PG synthesis and cortisol to inhibit it: cortisol has, however, no inhibitory effect on basal PG synthesis. In reducing prolactin-stimulated PG synthesis cortisol is 1000-2000 times more potent than indomethacin on a molar basis. We suggest that the regulation of intracellular PG levels is the mechanism of the so-called \"permissive\" actions of these hormones. These concepts could prove important in the understanding of many aspects of physiology and pathophysiology including diurnal and seasonal changes in hormone responsiveness. They are also relevant to the use of established drugs and the design of new ones.", "PMID": 967066}
{"id": "pubmed23n0032_6949", "title": "Physiologic profiles in circulatory support.", "content": "Measurements of hemodynamic, oxygen transport, and tissue utilization functions were incorporated into a physiologic profile assessment system in order to evaluate patients who are candidates for mechanical support of the failing heart. Circulatory function was assessed in patients with acute myocardial infarction associated with diminished peripheral perfusion and compared to an age-matched, normal patient group. Serial studies performed prior to and during intra-aortic balloon counterpulsation provide a means to assess circulatory status accurately and document the efficacy of therapeutic interventions.", "contents": "Physiologic profiles in circulatory support. Measurements of hemodynamic, oxygen transport, and tissue utilization functions were incorporated into a physiologic profile assessment system in order to evaluate patients who are candidates for mechanical support of the failing heart. Circulatory function was assessed in patients with acute myocardial infarction associated with diminished peripheral perfusion and compared to an age-matched, normal patient group. Serial studies performed prior to and during intra-aortic balloon counterpulsation provide a means to assess circulatory status accurately and document the efficacy of therapeutic interventions.", "PMID": 967073}
{"id": "pubmed23n0032_6950", "title": "Circulatory support with a left-heart assist device after intracardiac operation: design concepts and management techniques.", "content": "A simple left-heart assist device was developed to reduce left ventricular preload while simultaneously increasing total systemic blood flow. It consists of special cannulas connected to a simple extracorporeal tubing loop and roller pump, designed to permit bypass of as much as 5 liters of blood per minute from left atrium to ascending aorta. Employed in 15 patients with advanced heart disease who were in low cardiac output following repair, the system was proven effective. An asset of the device is the ability to subsequently separate the patient from the device without need to reenter the thorax or abdomen.", "contents": "Circulatory support with a left-heart assist device after intracardiac operation: design concepts and management techniques. A simple left-heart assist device was developed to reduce left ventricular preload while simultaneously increasing total systemic blood flow. It consists of special cannulas connected to a simple extracorporeal tubing loop and roller pump, designed to permit bypass of as much as 5 liters of blood per minute from left atrium to ascending aorta. Employed in 15 patients with advanced heart disease who were in low cardiac output following repair, the system was proven effective. An asset of the device is the ability to subsequently separate the patient from the device without need to reenter the thorax or abdomen.", "PMID": 967074}
{"id": "pubmed23n0032_6951", "title": "Return to work after a myocardial infarction: evaluation of planned rehabilitation and of a predictive rating scale.", "content": "This paper reports the first recorded controlled trial of cardiac rehabilitation after myocardial infarction in men of working age, viewed as a team intervention effort to facilitate the patient's return to normal work.  Our results show that this intervention is helpful in returning to jobs which they can handle successfully men who would otherwise be at risk of remaining unemployed. A previously developed rating scale for predicting return to work after myocardial infarction was used and reevaluated. Employment and occupational level at admission to hospital, work history, availability of the previous job, educational level, family and social stability, age at which regular cigarette smoking commenced, and level of anxiety and depression on a personality scale proved highly predictive.", "contents": "Return to work after a myocardial infarction: evaluation of planned rehabilitation and of a predictive rating scale. This paper reports the first recorded controlled trial of cardiac rehabilitation after myocardial infarction in men of working age, viewed as a team intervention effort to facilitate the patient's return to normal work.  Our results show that this intervention is helpful in returning to jobs which they can handle successfully men who would otherwise be at risk of remaining unemployed. A previously developed rating scale for predicting return to work after myocardial infarction was used and reevaluated. Employment and occupational level at admission to hospital, work history, availability of the previous job, educational level, family and social stability, age at which regular cigarette smoking commenced, and level of anxiety and depression on a personality scale proved highly predictive.", "PMID": 967080}
{"id": "pubmed23n0032_6952", "title": "Mobile laboratory for respiratory surveys in industry.", "content": "This paper describes the design and performance of a self-contained mobile respiratory laboratory used for the carrying out of complex lung function tests in epidemiological studies of respiratory disease in industry. The laboratory is equipped to perform all conventional lung function tests other than those involving arterial puncture or radioactive isotopes. It has been shown to have a high level of employee acceptability and technical accuracy.", "contents": "Mobile laboratory for respiratory surveys in industry. This paper describes the design and performance of a self-contained mobile respiratory laboratory used for the carrying out of complex lung function tests in epidemiological studies of respiratory disease in industry. The laboratory is equipped to perform all conventional lung function tests other than those involving arterial puncture or radioactive isotopes. It has been shown to have a high level of employee acceptability and technical accuracy.", "PMID": 967081}
{"id": "pubmed23n0032_6953", "title": "Hemodynamic alterations with a reversed unidirectional intra-aortic balloon.", "content": "The hemodynamic effects of dual-chambered unidirectional balloon pumping in alternate directions were studied in 44 trials conducted on 8 dogs. Results indicate an increase in cardiac output in normal dogs with downstream pumping by unidirectional intra-aortic balloon, which may have important implications for the treatment of low output cardiac failure.", "contents": "Hemodynamic alterations with a reversed unidirectional intra-aortic balloon. The hemodynamic effects of dual-chambered unidirectional balloon pumping in alternate directions were studied in 44 trials conducted on 8 dogs. Results indicate an increase in cardiac output in normal dogs with downstream pumping by unidirectional intra-aortic balloon, which may have important implications for the treatment of low output cardiac failure.", "PMID": 967072}
{"id": "pubmed23n0032_6954", "title": "Medical manpower-the Australian scene.", "content": "Medical manpower projections indicate that by 1991 there will be one doctor to 500 population instead of the 1: 721 ratio in 1976. There are many variables; projections, while hazardous, are necessary for planning because of the long lead time. Productivity is equally important. There is no evidence that the rapidly rising female intake will reduce productivity in the next 15 years.", "contents": "Medical manpower-the Australian scene. Medical manpower projections indicate that by 1991 there will be one doctor to 500 population instead of the 1: 721 ratio in 1976. There are many variables; projections, while hazardous, are necessary for planning because of the long lead time. Productivity is equally important. There is no evidence that the rapidly rising female intake will reduce productivity in the next 15 years.", "PMID": 967082}
{"id": "pubmed23n0032_6955", "title": "Primary oxalosis with myocardial involvement and heart block.", "content": "An unusual case is described of primary oxalosis with renal failure and cardiac involvement with complete heart block. Although cardiac involvement rarely occurs, it nevertheless requires evaluation because of the therapeutic implications.", "contents": "Primary oxalosis with myocardial involvement and heart block. An unusual case is described of primary oxalosis with renal failure and cardiac involvement with complete heart block. Although cardiac involvement rarely occurs, it nevertheless requires evaluation because of the therapeutic implications.", "PMID": 967083}
{"id": "pubmed23n0032_6956", "title": "The Windkesselventricle with guiding balloon as a new approach for assisted circulation.", "content": "A simple method was developed to aid the patient with low cardiac output syndrome following cardiac surgery. The concept was shown to be feasible in electric circulatory analog studies and verified in 20 dog experiments. A Dacron graft (end-to-side) through the right second intercostal space connects the ascending aorta to the subcutaneously implanted, ellipsoidal-shaped artifical ventricle. A spherical polyurethane balloon is positioned in the aorta distal to the Dacron graft via the femoral artery. The ventricle and balloon are pneumatically driven synchronously with the ECG. In natural systole the balloon is inflated, occluding the aorta, and the artificial ventricle sucks the entire stroke volume. In natural diastole the balloon deflates and the artificial ventricle ejects the blood into the peripheral arteries. With this system it is possible to maintain a normal systemic pressure and have high hemodynamic efficiency. The left ventricular systolic pressure is 85 percent unloaded. The systolic wave is turned 180 degrees to the natural. After treatment, the device can be removed without thoracotomy.", "contents": "The Windkesselventricle with guiding balloon as a new approach for assisted circulation. A simple method was developed to aid the patient with low cardiac output syndrome following cardiac surgery. The concept was shown to be feasible in electric circulatory analog studies and verified in 20 dog experiments. A Dacron graft (end-to-side) through the right second intercostal space connects the ascending aorta to the subcutaneously implanted, ellipsoidal-shaped artifical ventricle. A spherical polyurethane balloon is positioned in the aorta distal to the Dacron graft via the femoral artery. The ventricle and balloon are pneumatically driven synchronously with the ECG. In natural systole the balloon is inflated, occluding the aorta, and the artificial ventricle sucks the entire stroke volume. In natural diastole the balloon deflates and the artificial ventricle ejects the blood into the peripheral arteries. With this system it is possible to maintain a normal systemic pressure and have high hemodynamic efficiency. The left ventricular systolic pressure is 85 percent unloaded. The systolic wave is turned 180 degrees to the natural. After treatment, the device can be removed without thoracotomy.", "PMID": 967076}
{"id": "pubmed23n0032_6957", "title": "Intra-aortic balloon assist for cardiogenic shock and ischemic states at Massachusetts General Hospital.", "content": "Intra-aortic balloon pumping is used at MGH for the reversal of acute myocardial ischemia in those patients not responding to usual medical means. The survival rate of surgical intervention in patients in cardiogenic shock was significantly increased in patients who had been balloon-pumped prior to surgery. The use of the intra-aortic balloon assist early in the postoperative patient showing low cardiac output state not only served to improve hemodynamics but also led to the preservation of the myocardium and ultimate improved survival. The balloon assist was initiated to control the ischemic states in patients with severe unstable angina. Used in association with nitroprusside and propranolol, this method produced marked improvement and reduction of pain in patients. Indications are that intra-aortic balloon counterpulsation at the present time is the safest and most predictable means of controlling acute ischemia in patients who are resistant to standard medical intervention.", "contents": "Intra-aortic balloon assist for cardiogenic shock and ischemic states at Massachusetts General Hospital. Intra-aortic balloon pumping is used at MGH for the reversal of acute myocardial ischemia in those patients not responding to usual medical means. The survival rate of surgical intervention in patients in cardiogenic shock was significantly increased in patients who had been balloon-pumped prior to surgery. The use of the intra-aortic balloon assist early in the postoperative patient showing low cardiac output state not only served to improve hemodynamics but also led to the preservation of the myocardium and ultimate improved survival. The balloon assist was initiated to control the ischemic states in patients with severe unstable angina. Used in association with nitroprusside and propranolol, this method produced marked improvement and reduction of pain in patients. Indications are that intra-aortic balloon counterpulsation at the present time is the safest and most predictable means of controlling acute ischemia in patients who are resistant to standard medical intervention.", "PMID": 967075}
{"id": "pubmed23n0032_6958", "title": "Anatomy of the lumbar spine.", "content": "The macroscopic courses of the posterior primary rami in the lumbar spine are described and illustrated from fresh cadaver dissections.", "contents": "Anatomy of the lumbar spine. The macroscopic courses of the posterior primary rami in the lumbar spine are described and illustrated from fresh cadaver dissections.", "PMID": 967084}
{"id": "pubmed23n0032_6959", "title": "How did you guess? Or, what do multiple-choice questions measure?", "content": "Multiple-choice questions classified as requiring problem-solving skills have been interpreted as measuring problem-solving skills within students, with the implicit hypothesis that questions needing an increasingly complex intellectual process should present increasing difficulty to the student. This hypothesis was tested in a 150-question paper taken by 721 students in seven Australian medical schools. No correlation was observed between difficulty and assigned process. Consequently, the question-answering process was explored with a group of final-year students. Anecdotal recall by students gave heavy weight to knowledge rather than problem solving in answering these questions. Assignment of the 150 questions to the classification by three teachers and six students showed their congruence to be a little above random probability.", "contents": "How did you guess? Or, what do multiple-choice questions measure? Multiple-choice questions classified as requiring problem-solving skills have been interpreted as measuring problem-solving skills within students, with the implicit hypothesis that questions needing an increasingly complex intellectual process should present increasing difficulty to the student. This hypothesis was tested in a 150-question paper taken by 721 students in seven Australian medical schools. No correlation was observed between difficulty and assigned process. Consequently, the question-answering process was explored with a group of final-year students. Anecdotal recall by students gave heavy weight to knowledge rather than problem solving in answering these questions. Assignment of the 150 questions to the classification by three teachers and six students showed their congruence to be a little above random probability.", "PMID": 967086}
{"id": "pubmed23n0032_6960", "title": "[Breast affections requiring surgery: diagnosis from the view-point of a radiologist (author's transl)].", "content": "Examinations by X-rays are the best method to detect early carcinomas of the breast and precancerous alterations. In many cases it is possible to avoid operations by pneumocystography. An unsuspected result by pneumocytostography permits to depart from the principle to remove every palpable tumor. Nevertheless the X-ray examinations have some limits. Therefore it is necessary for the radiologist to know exactly the clinical state. Furthermore there must be a very good cooperation between the radiologist, the surgeon and the histologist. Problems originated from the appearance of so-called suspicious microcalcifications visible in well made mammograms. The sooner the microcalcifications are excised the more it is possible to discover early carcinomas and precancerous alterations but also harmless variations. Pathological secretion from the nipple is an indication for galactography. If there are pathological alterations in the ducts it is necessary in order to avoid mistakes in diagnosis to excise and examine the specimen by very reliable methods. The results from thermographical and cytological investigations are sometimes of value for the decision to perform an operation but not for the decision to omit the histological examination.", "contents": "[Breast affections requiring surgery: diagnosis from the view-point of a radiologist (author's transl)]. Examinations by X-rays are the best method to detect early carcinomas of the breast and precancerous alterations. In many cases it is possible to avoid operations by pneumocystography. An unsuspected result by pneumocytostography permits to depart from the principle to remove every palpable tumor. Nevertheless the X-ray examinations have some limits. Therefore it is necessary for the radiologist to know exactly the clinical state. Furthermore there must be a very good cooperation between the radiologist, the surgeon and the histologist. Problems originated from the appearance of so-called suspicious microcalcifications visible in well made mammograms. The sooner the microcalcifications are excised the more it is possible to discover early carcinomas and precancerous alterations but also harmless variations. Pathological secretion from the nipple is an indication for galactography. If there are pathological alterations in the ducts it is necessary in order to avoid mistakes in diagnosis to excise and examine the specimen by very reliable methods. The results from thermographical and cytological investigations are sometimes of value for the decision to perform an operation but not for the decision to omit the histological examination.", "PMID": 967092}
{"id": "pubmed23n0032_6961", "title": "[Vulvitis due to ancylostoma duodenale (author's transl)].", "content": "There is reported about a case of vulvitis, which was resistant to usual forms of therapy. Infections as such of Candida or of Trichomonas as well as diabetes could be excluded. But there could be proved an incidence of Ancylostoma duodenale, which is nowadays more often to be observed. Adequate therapy was successful.", "contents": "[Vulvitis due to ancylostoma duodenale (author's transl)]. There is reported about a case of vulvitis, which was resistant to usual forms of therapy. Infections as such of Candida or of Trichomonas as well as diabetes could be excluded. But there could be proved an incidence of Ancylostoma duodenale, which is nowadays more often to be observed. Adequate therapy was successful.", "PMID": 967093}
{"id": "pubmed23n0032_6962", "title": "Nervous signs in bovine coccidiosis.", "content": "In 2 outbreaks of coccidiosis due to E bovis and/or E zurnii infection in Canadian cattle, nervous signs included opisthotonos, medial strabismus, hypersensitivity, tetanic spasms, and convulsions. All of the affected animals died in convulsions after an illness of one to several days during which time they showed periodic nervous signs. Necropsy revealed a very severe enteritis with the most severe lesions in the spiral colon. Much of the intestinal mucosa in this area had been destroyed by the parasite. None of several suggested causes of such nervous signs was indicated by laboratory findings, but the possibility of toxins produced by the coccidia could not be ruled out.", "contents": "Nervous signs in bovine coccidiosis. In 2 outbreaks of coccidiosis due to E bovis and/or E zurnii infection in Canadian cattle, nervous signs included opisthotonos, medial strabismus, hypersensitivity, tetanic spasms, and convulsions. All of the affected animals died in convulsions after an illness of one to several days during which time they showed periodic nervous signs. Necropsy revealed a very severe enteritis with the most severe lesions in the spiral colon. Much of the intestinal mucosa in this area had been destroyed by the parasite. None of several suggested causes of such nervous signs was indicated by laboratory findings, but the possibility of toxins produced by the coccidia could not be ruled out.", "PMID": 967151}
{"id": "pubmed23n0032_6963", "title": "Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii.", "content": "Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.", "contents": "Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii. Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.", "PMID": 967156}
{"id": "pubmed23n0032_6964", "title": "Recombination between mutants of Phycomyces with abnormal phototropism.", "content": "Sexual crosses were studied between mutants of Phycomyces blakesleeanus with abnormal phototropism (phenotype mad). Recombination frequencies were determined among five genes madA to madE. No clear evidence was found for linkage between any of the genes. Inconsistent results in crosses involving madC are attributed to nonisogenicity between the particular strains used. One mad strain was discovered to be a double mutant. A new gene, tentatively designated madG, was segregated from a cross involving that strain.", "contents": "Recombination between mutants of Phycomyces with abnormal phototropism. Sexual crosses were studied between mutants of Phycomyces blakesleeanus with abnormal phototropism (phenotype mad). Recombination frequencies were determined among five genes madA to madE. No clear evidence was found for linkage between any of the genes. Inconsistent results in crosses involving madC are attributed to nonisogenicity between the particular strains used. One mad strain was discovered to be a double mutant. A new gene, tentatively designated madG, was segregated from a cross involving that strain.", "PMID": 967157}
{"id": "pubmed23n0032_6965", "title": "A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis.", "content": "A new genetic nomenclature for the macromolecules involved in bacterial protein synthesis is proposed and explained. Genes for ribosomal proteins are designated rsp, rpl and rpm while genes for ribosomal RNAs are rrs and rrl. Protein synthesis factors and ribosome assembly and modification activities are also consistantly named.", "contents": "A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis. A new genetic nomenclature for the macromolecules involved in bacterial protein synthesis is proposed and explained. Genes for ribosomal proteins are designated rsp, rpl and rpm while genes for ribosomal RNAs are rrs and rrl. Protein synthesis factors and ribosome assembly and modification activities are also consistantly named.", "PMID": 967155}
{"id": "pubmed23n0032_6966", "title": "The product of the ade1: gene in Schizosaccharomyces pombe: a bifunctional enzyme catalysing two distinct steps in purine biosynthesis.", "content": "The assignment of the known ade genes to steps in purine biosynthesis in Schizosaccharomyces pombe has been completed with the demonstration that an ade3 mutants lacks FGAR amidotransferase, ade1A mutants lack GAR synthetase and ade1B mutants lack AIR synthetase. A comparison of enzyme activity with map position for ade1 mutants shows that (1) complementing ade1A mutants lack GAR synthetase but posses wild type amounts of AIR synthetase, (2) complementing ade1B mutants lack AIR synthetase but posses variable amounts of GAR synthetase, (3) non-complementing mutants lack both activities. In wild type strains the two activities fractionate together throughout a hundred-fold purification. Hence the ade1 gene appears to code for a bifunctional enzyme catalysing two distinct steps in purine biosynthesis. The two activities are catalysed by two different regions of the polypeptide chain which can be altered independently by mutation. Gel filtration studies on partially purified enzymes from wild type and various complementing mutant strains, indicate that the bifunctional enzyme is a multimer consisting of between four and six sub-units of 40,000 daltons each. GAR synthetase activity is associated with both the monomeric and multimeric forms but AIR synthetase is only associated with the multimer. A comparison of enzyme levels between diploids and their original complementing haploid strains suggests that complementation is due to hybrid enzyme formation.", "contents": "The product of the ade1: gene in Schizosaccharomyces pombe: a bifunctional enzyme catalysing two distinct steps in purine biosynthesis. The assignment of the known ade genes to steps in purine biosynthesis in Schizosaccharomyces pombe has been completed with the demonstration that an ade3 mutants lacks FGAR amidotransferase, ade1A mutants lack GAR synthetase and ade1B mutants lack AIR synthetase. A comparison of enzyme activity with map position for ade1 mutants shows that (1) complementing ade1A mutants lack GAR synthetase but posses wild type amounts of AIR synthetase, (2) complementing ade1B mutants lack AIR synthetase but posses variable amounts of GAR synthetase, (3) non-complementing mutants lack both activities. In wild type strains the two activities fractionate together throughout a hundred-fold purification. Hence the ade1 gene appears to code for a bifunctional enzyme catalysing two distinct steps in purine biosynthesis. The two activities are catalysed by two different regions of the polypeptide chain which can be altered independently by mutation. Gel filtration studies on partially purified enzymes from wild type and various complementing mutant strains, indicate that the bifunctional enzyme is a multimer consisting of between four and six sub-units of 40,000 daltons each. GAR synthetase activity is associated with both the monomeric and multimeric forms but AIR synthetase is only associated with the multimer. A comparison of enzyme levels between diploids and their original complementing haploid strains suggests that complementation is due to hybrid enzyme formation.", "PMID": 967158}
{"id": "pubmed23n0032_6967", "title": "Lethal and mutagenic effects of nitrosoguanidine on synchronized Chlamydomonas.", "content": "The lethal and mutagenic effects of 5 mug/ml N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were maximal during the nuclear S-period of synchronously grown Chlamydomonas reinhardtii. This was revealed by a 50% drop in survival and a 50- to 100-fold increase in the recovery of slow-growth mutants (up to 40% of the survivors) which were first recognized as small colonies on agar medium. Partial characterization of these isolates revealed about 50% to be stable on subculture, and several were demonstrated to be either acetate-dependent, dark-lethal (light-dependent), or acetate-sensitive mutants. There was no significant increase of lethality or of slow-growth mutants correlated with treatment during the chloroplast DNA replication phase of the cell-cycle. The results of genetic analysis with 13 mutants induced during the nuclear S-period were consistent with their nuclear origin. These analyses were hampered by the high proportion of lethality among the progeny of most crosses. It is concluded that the enhanced mutant induction among nuclear S-phase cells may indicate preferential mutagenesis of replication fork DNA and induction of multiple-closely-linked mutations, as in some bacteria. Consequently, for C. reinhardtii, caution should be exercised in drawing relationships between abnormal behavioral and biochemical phenotypes in MNNG-induced mutants.", "contents": "Lethal and mutagenic effects of nitrosoguanidine on synchronized Chlamydomonas. The lethal and mutagenic effects of 5 mug/ml N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were maximal during the nuclear S-period of synchronously grown Chlamydomonas reinhardtii. This was revealed by a 50% drop in survival and a 50- to 100-fold increase in the recovery of slow-growth mutants (up to 40% of the survivors) which were first recognized as small colonies on agar medium. Partial characterization of these isolates revealed about 50% to be stable on subculture, and several were demonstrated to be either acetate-dependent, dark-lethal (light-dependent), or acetate-sensitive mutants. There was no significant increase of lethality or of slow-growth mutants correlated with treatment during the chloroplast DNA replication phase of the cell-cycle. The results of genetic analysis with 13 mutants induced during the nuclear S-period were consistent with their nuclear origin. These analyses were hampered by the high proportion of lethality among the progeny of most crosses. It is concluded that the enhanced mutant induction among nuclear S-phase cells may indicate preferential mutagenesis of replication fork DNA and induction of multiple-closely-linked mutations, as in some bacteria. Consequently, for C. reinhardtii, caution should be exercised in drawing relationships between abnormal behavioral and biochemical phenotypes in MNNG-induced mutants.", "PMID": 967159}
{"id": "pubmed23n0032_6968", "title": "Genetic evidence of ribosomal antisuppressors in Podospora anserina.", "content": "Antisuppressors were screened for with the help of informational suppressors in Podospores anserina. Four mutations in the AS1 locus and two in the AS2 locus were isolated, using allele non specific suppressors supposed to be ribosomal ambiguity mutations. Four mutations in the AS3 locus and 45 in the AS4 locus were obtained, using a nonsense (t-RNA like) suppressor. All antisuppressors are partially dominant. Most mutations in the AS4 locus are lethal. The four mutants at the AS3 locus and 6 out of the 8 viable mutants at the AS4 locus are cold sensitive. Phenotypic properties and action spectra of the antisuppressors suggest that they are restrictive ribosomal mutations.", "contents": "Genetic evidence of ribosomal antisuppressors in Podospora anserina. Antisuppressors were screened for with the help of informational suppressors in Podospores anserina. Four mutations in the AS1 locus and two in the AS2 locus were isolated, using allele non specific suppressors supposed to be ribosomal ambiguity mutations. Four mutations in the AS3 locus and 45 in the AS4 locus were obtained, using a nonsense (t-RNA like) suppressor. All antisuppressors are partially dominant. Most mutations in the AS4 locus are lethal. The four mutants at the AS3 locus and 6 out of the 8 viable mutants at the AS4 locus are cold sensitive. Phenotypic properties and action spectra of the antisuppressors suggest that they are restrictive ribosomal mutations.", "PMID": 967160}
{"id": "pubmed23n0032_6969", "title": "Insect cytochrome P-450.", "content": "Two approaches may be used to study the function of cytochrome P-450 in insects: (a) an evaluation of the spectral and catalytic properties of the hemoprotein while associated with microsomal membranes; (b) the solubilization, resolution and purification of the microsomal mixed-function oxidase system. The first approach has provided some understanding of the biochemical factors involved in the metabolism of a variety of compounds, including pesticides, drugs, hormones and many other xenobiotics. However, solubilization of the monooxygenase system allows the study of each of its components individually, providing a better insight on the sequence of events leading to the hydroxylation of a substrate, the type of intermediates formed, and the rate-limiting step(s). This report discusses studies carried out with the monooxygenase system associated with microsomal membranes, as well as procedures to solubilize and partially purify its components from housefly microsomes. The latter involves solubilization with either Triton X-100 or sodium cholate, followed by either ammonium sulfate fractionation, Sephadex G-200, DEAE-Sephadex A-50 column chromatography or by omega-amino-n-octyl-Sepharose 4B affinity chromatography. These procedures have shown that two cytochrome P-450 species (P-450 and P-450I) are present in microsomes isolated from a resistant housefly strain. Induction with either naphthalene or phenobarbital appears to increase cytochrome P-450I preferentially.", "contents": "Insect cytochrome P-450. Two approaches may be used to study the function of cytochrome P-450 in insects: (a) an evaluation of the spectral and catalytic properties of the hemoprotein while associated with microsomal membranes; (b) the solubilization, resolution and purification of the microsomal mixed-function oxidase system. The first approach has provided some understanding of the biochemical factors involved in the metabolism of a variety of compounds, including pesticides, drugs, hormones and many other xenobiotics. However, solubilization of the monooxygenase system allows the study of each of its components individually, providing a better insight on the sequence of events leading to the hydroxylation of a substrate, the type of intermediates formed, and the rate-limiting step(s). This report discusses studies carried out with the monooxygenase system associated with microsomal membranes, as well as procedures to solubilize and partially purify its components from housefly microsomes. The latter involves solubilization with either Triton X-100 or sodium cholate, followed by either ammonium sulfate fractionation, Sephadex G-200, DEAE-Sephadex A-50 column chromatography or by omega-amino-n-octyl-Sepharose 4B affinity chromatography. These procedures have shown that two cytochrome P-450 species (P-450 and P-450I) are present in microsomes isolated from a resistant housefly strain. Induction with either naphthalene or phenobarbital appears to increase cytochrome P-450I preferentially.", "PMID": 967161}
{"id": "pubmed23n0032_6970", "title": "Modification of calf lens crystallins as determined by gel electrophoresis.", "content": "Variations in size and charge of calf lens proteins, particularly gamma crystallins, were studied by polyacrylamide gel electrophoresis. Exposure of gamma crystallins to near-UV light in the presence of L-tryptophan produces species of higher electrophoretic mobility and higher retardation. Treatment with urea and sulfonation also produced changes in the retardation co-efficient. The increase of retardation co-efficient of gamma crystallin is interpreted to be a result of conformational changes. Gamma crystallins are particularly sensitive to photo-modification, and this process may be associated with age-related changes in the lens.", "contents": "Modification of calf lens crystallins as determined by gel electrophoresis. Variations in size and charge of calf lens proteins, particularly gamma crystallins, were studied by polyacrylamide gel electrophoresis. Exposure of gamma crystallins to near-UV light in the presence of L-tryptophan produces species of higher electrophoretic mobility and higher retardation. Treatment with urea and sulfonation also produced changes in the retardation co-efficient. The increase of retardation co-efficient of gamma crystallin is interpreted to be a result of conformational changes. Gamma crystallins are particularly sensitive to photo-modification, and this process may be associated with age-related changes in the lens.", "PMID": 967162}
{"id": "pubmed23n0032_6971", "title": "Efficacy of law enforcement procedures concerning alcohol, drugs, and driving.", "content": "Fatality rates in autobomile crashes vary widely from nation to nation. For instance, in 1970 Japan reported a rate of 11.4 and the United States 2.6. Finland stood about midway with 6.2 fatalities per 100 million vehicle kilometers 1970 (OECD, 1974). These rates reflect all fatal crash causes. The alcohol rates are quite imprecise for many nations but a comparison of the Finnish and United States figures reveals some interesting facts. The United States rate of alcohol involvement in highway deaths is about 50% (of a rate of 2.6). The Finnish rate of alcohol involvement is 27% (of a rate of 6.2). Thus the alcohol rate for Finland is about 1.7, and for the United States 1.3. These percentages provide a guide for enforcement officials. The higher the percentage, the higher the priority. Moreover, the role of traffic deaths in overall mortality is important. For instance, in Japan each year 25 traffic deaths occur in each 100,000 of population, while in the United Kingdom the figure is 13 (1970 figures; OECD, 1974). Another factor is available countermeasures. Safety features on cars, quality of roads, driver training, and cultural attitudes toward cars all contribute. Driver behavior controlled by law enforcement is a very important factor. There is evidence that enforcement of drunken driving laws is extremely lax even in those nations claiming vigorous programs. An enforcement program that will screen the corporate whole of the driving population on an optimal basis will identify those drivers whose drinking is a problem for treatment ranging from punitive to therapeutic on the basis of their needs and at the same time bring about a general deterrence because of a perception of the high risk of being apprehended.", "contents": "Efficacy of law enforcement procedures concerning alcohol, drugs, and driving. Fatality rates in autobomile crashes vary widely from nation to nation. For instance, in 1970 Japan reported a rate of 11.4 and the United States 2.6. Finland stood about midway with 6.2 fatalities per 100 million vehicle kilometers 1970 (OECD, 1974). These rates reflect all fatal crash causes. The alcohol rates are quite imprecise for many nations but a comparison of the Finnish and United States figures reveals some interesting facts. The United States rate of alcohol involvement in highway deaths is about 50% (of a rate of 2.6). The Finnish rate of alcohol involvement is 27% (of a rate of 6.2). Thus the alcohol rate for Finland is about 1.7, and for the United States 1.3. These percentages provide a guide for enforcement officials. The higher the percentage, the higher the priority. Moreover, the role of traffic deaths in overall mortality is important. For instance, in Japan each year 25 traffic deaths occur in each 100,000 of population, while in the United Kingdom the figure is 13 (1970 figures; OECD, 1974). Another factor is available countermeasures. Safety features on cars, quality of roads, driver training, and cultural attitudes toward cars all contribute. Driver behavior controlled by law enforcement is a very important factor. There is evidence that enforcement of drunken driving laws is extremely lax even in those nations claiming vigorous programs. An enforcement program that will screen the corporate whole of the driving population on an optimal basis will identify those drivers whose drinking is a problem for treatment ranging from punitive to therapeutic on the basis of their needs and at the same time bring about a general deterrence because of a perception of the high risk of being apprehended.", "PMID": 967163}
{"id": "pubmed23n0032_6972", "title": "Characteristics of driving in relation to the drug and alcohol use of Finnish outpatients.", "content": "A questionnaire was administered to 765 rheumatoid arthritic, 715 tubercular and 1,050 psychiatric outpatients and to 587 control subjects concerning their use of drugs and alcohol, driving habits and traffic accident involvement. The driving populations of all groups were matched as to their age and area of residence. The results of the study show a relatively very small number of driving licence holders among rheumatoid and psychiatric patients whereas the tubercular group includes as many driving licences as the control group. Also, the annual driving experience of the rheumatoid arthritic and psychiatric patients was low. The main reason for driving was recreation, and the driving mainly took place on highways. Excluding the psychiatric patients, the patient groups had been involved less often in traffic accidents that the controls. The use of drugs correlated inversely with driving: the more you used drugs the less you drove. Also the consumption of alcohol increased in direct proportion to the number of kilometres driven: the greater the alcohol consumption per drinking session, the more you drove annually and were involved in accidents.", "contents": "Characteristics of driving in relation to the drug and alcohol use of Finnish outpatients. A questionnaire was administered to 765 rheumatoid arthritic, 715 tubercular and 1,050 psychiatric outpatients and to 587 control subjects concerning their use of drugs and alcohol, driving habits and traffic accident involvement. The driving populations of all groups were matched as to their age and area of residence. The results of the study show a relatively very small number of driving licence holders among rheumatoid and psychiatric patients whereas the tubercular group includes as many driving licences as the control group. Also, the annual driving experience of the rheumatoid arthritic and psychiatric patients was low. The main reason for driving was recreation, and the driving mainly took place on highways. Excluding the psychiatric patients, the patient groups had been involved less often in traffic accidents that the controls. The use of drugs correlated inversely with driving: the more you used drugs the less you drove. Also the consumption of alcohol increased in direct proportion to the number of kilometres driven: the greater the alcohol consumption per drinking session, the more you drove annually and were involved in accidents.", "PMID": 967164}
{"id": "pubmed23n0032_6973", "title": "Alcohol and highway crashes. Closing the gap between epidemiology and experimentation.", "content": "It is frequently observed that alcohol-impaired drivers involved in certain types of crashes apparently 'couldn't stop in time'. However accurate this observation, it is simply a description and is not an adequate explanation. Therein lies the basis for the gap between epidemiology and experimentation. If alcohol actually does degrade a motorist's performance and increase the probability of his being responsible for a fatal crash, then alcohol-induced changes in driving behavior should be manifest and should be measurable. However, no controlled study has previously been conducted to obtain systematic but unobtrusive data on the actual influences of alcohol upon real-world driving behavior in its natural environment. One recently completed field study is reported which was designed to provide such data by means of unobtrusive electronic measures of nocturnal driving performance. In the present paper, to illustrate one approach to closing the gap between epidemiology and experimentation, one combination variable was selected which lends itself readily to comparison across the full spectrum of alcohol investigation: reaction time and braking performance. A review of the literature concerning this interrelated variable examined alcohol influences upon reaction time as investigated in laboratory, simulator, and instrumented car experiments, as well as alcohol influences upon braking performance in instrumented car experiments and in our recent field study involving unobtrusive electronic measures. The reviewed experiments were interpreted in terms of a conceptualization of the information-processing sequence which leads up to brake use. On the basis of the reviewed results, it was concluded that alcohol increases reaction time (both simple and choice) appreciably more in driving situations than in laboratory experiments. It was also concluded that a consistent alcohol impairment of the qualitative aspects of braking performance is manifest in driving situations, for example, as reflected by changes in brake-pressure modulation. More specifically, the braking performance of motorists and subjects at high BACs is abrupt, unsmooth, and less controlled than that of sober motorists or the same subjects with no alcohol. It was concluded that at least in terms of quality, alcohol impairs response implementation of the final stage of the information-processing sequence. It was hypothesized that the abrupt, lower quality braking performance observed at high BACs results--at least in part--from less time remaining available for stopping because the information-processing time has been increased by alcohol at some previous stage--apparently the response-selection stage. At the most general level, it was suggested that high BACs both increase the time necessary to begin applying the brakes, as well as reduce the degree of control in the actual use of the brakes during the course of stopping. These two factors in combination probably account for a large part of the alcohol contribution to highway crashes.", "contents": "Alcohol and highway crashes. Closing the gap between epidemiology and experimentation. It is frequently observed that alcohol-impaired drivers involved in certain types of crashes apparently 'couldn't stop in time'. However accurate this observation, it is simply a description and is not an adequate explanation. Therein lies the basis for the gap between epidemiology and experimentation. If alcohol actually does degrade a motorist's performance and increase the probability of his being responsible for a fatal crash, then alcohol-induced changes in driving behavior should be manifest and should be measurable. However, no controlled study has previously been conducted to obtain systematic but unobtrusive data on the actual influences of alcohol upon real-world driving behavior in its natural environment. One recently completed field study is reported which was designed to provide such data by means of unobtrusive electronic measures of nocturnal driving performance. In the present paper, to illustrate one approach to closing the gap between epidemiology and experimentation, one combination variable was selected which lends itself readily to comparison across the full spectrum of alcohol investigation: reaction time and braking performance. A review of the literature concerning this interrelated variable examined alcohol influences upon reaction time as investigated in laboratory, simulator, and instrumented car experiments, as well as alcohol influences upon braking performance in instrumented car experiments and in our recent field study involving unobtrusive electronic measures. The reviewed experiments were interpreted in terms of a conceptualization of the information-processing sequence which leads up to brake use. On the basis of the reviewed results, it was concluded that alcohol increases reaction time (both simple and choice) appreciably more in driving situations than in laboratory experiments. It was also concluded that a consistent alcohol impairment of the qualitative aspects of braking performance is manifest in driving situations, for example, as reflected by changes in brake-pressure modulation. More specifically, the braking performance of motorists and subjects at high BACs is abrupt, unsmooth, and less controlled than that of sober motorists or the same subjects with no alcohol. It was concluded that at least in terms of quality, alcohol impairs response implementation of the final stage of the information-processing sequence. It was hypothesized that the abrupt, lower quality braking performance observed at high BACs results--at least in part--from less time remaining available for stopping because the information-processing time has been increased by alcohol at some previous stage--apparently the response-selection stage. At the most general level, it was suggested that high BACs both increase the time necessary to begin applying the brakes, as well as reduce the degree of control in the actual use of the brakes during the course of stopping. These two factors in combination probably account for a large part of the alcohol contribution to highway crashes.", "PMID": 967165}
{"id": "pubmed23n0032_6974", "title": "A case-control study on alcohol as a risk factor in pedestrian accident. A preliminary report.", "content": "Alcohol as a risk factor in pedestrian accidents was studied so that the BACs were measured by gas chromatography in 341 accident patients and by ASD-breathalyzers in 682 sex-, site-, and time-matched pedestrians not involved in accidents. Alcohol was found in the blood in 53% of the accident cases and in 15% of the controls. The RR, if 1.0 at zero BAC, was 0.59 at BAC 0.3-0.5, 2.2 at 0.6-1.0, 6.8 at 1.1-1.5, 13 at 1.6-2.0, and 29 at BACs of over 2.0 g/l.", "contents": "A case-control study on alcohol as a risk factor in pedestrian accident. A preliminary report. Alcohol as a risk factor in pedestrian accidents was studied so that the BACs were measured by gas chromatography in 341 accident patients and by ASD-breathalyzers in 682 sex-, site-, and time-matched pedestrians not involved in accidents. Alcohol was found in the blood in 53% of the accident cases and in 15% of the controls. The RR, if 1.0 at zero BAC, was 0.59 at BAC 0.3-0.5, 2.2 at 0.6-1.0, 6.8 at 1.1-1.5, 13 at 1.6-2.0, and 29 at BACs of over 2.0 g/l.", "PMID": 967167}
{"id": "pubmed23n0032_6975", "title": "Precision, accuracy and relevance of breath alcohol measurements.", "content": "The methods employed for evaluating the performance of some currently available breath alcohol devices are described. A program consisting of in vitro experiments, extensive in vivo laboratory studies, controlled field studies, and finally roadside random testing has been used. The importance of considering the underlying physiological mechanisms particularly with regard to the blood/breath partition ratio and its variability is emphasized. An important finding is that the relationship varies with the phase of alcohol metabolism in a regular and systematic way. Some statistical techniques are outlined for calculating the precision and accuracy of breath-testing instruments, which are important properties of a method to be used for medico-legal purposes.", "contents": "Precision, accuracy and relevance of breath alcohol measurements. The methods employed for evaluating the performance of some currently available breath alcohol devices are described. A program consisting of in vitro experiments, extensive in vivo laboratory studies, controlled field studies, and finally roadside random testing has been used. The importance of considering the underlying physiological mechanisms particularly with regard to the blood/breath partition ratio and its variability is emphasized. An important finding is that the relationship varies with the phase of alcohol metabolism in a regular and systematic way. Some statistical techniques are outlined for calculating the precision and accuracy of breath-testing instruments, which are important properties of a method to be used for medico-legal purposes.", "PMID": 967169}
{"id": "pubmed23n0032_6976", "title": "X-ray-induced mutation to 6-thioguanine resistance in cultured human diploid fibroblasts.", "content": "X-ray induced mutation to 6-thioguanine (6TG)-resistance was studied in early passage cultures of human diploid fibroblasts. The appearance of phenotypic induced mutants in irradiated cell populations was linearly related to the number of post-irradiation cell doublings and to the duration of the growth period prior to mutant selection; the maximum yield of X-ray induced mutants was observed when cells surviving radiation had completed 3--4 douplings (6--7 days growth) in non-selective medium. The maximum induced mutation frequency was linearly related to X-ray dose and the mutation rate was estimated to be 3.1-10(-7) mutations per viable cell per rad. The data obtained for X-ray induced mutations in cultured human diploid fibroblasts were compared with (a) similar experimental data obtained with established cell cultures and (b) with theoretical predictions of X-ray mutation rates in human germ cells.", "contents": "X-ray-induced mutation to 6-thioguanine resistance in cultured human diploid fibroblasts. X-ray induced mutation to 6-thioguanine (6TG)-resistance was studied in early passage cultures of human diploid fibroblasts. The appearance of phenotypic induced mutants in irradiated cell populations was linearly related to the number of post-irradiation cell doublings and to the duration of the growth period prior to mutant selection; the maximum yield of X-ray induced mutants was observed when cells surviving radiation had completed 3--4 douplings (6--7 days growth) in non-selective medium. The maximum induced mutation frequency was linearly related to X-ray dose and the mutation rate was estimated to be 3.1-10(-7) mutations per viable cell per rad. The data obtained for X-ray induced mutations in cultured human diploid fibroblasts were compared with (a) similar experimental data obtained with established cell cultures and (b) with theoretical predictions of X-ray mutation rates in human germ cells.", "PMID": 967185}
{"id": "pubmed23n0032_6977", "title": "Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential sample mammalian assay for mutagenesis.", "content": "Since mutagenic substances induce structural changes in DNA, studies were undertaken to determine whether substances would modify DNA replicative activity. DNA synthesis was quantitated 3.5 h after drug administration as uptake into DNA of a 30-min pulse of 10 muCi of [3H] thymidine. Combinations of methylurea (20000 mg/kg) and sodium nitrite (150 mg/kg) administered p.o. resulted in gastric synthesis of nitrosomethylurea and inhibited testicular DNA synthesis by 83%. Combinations of methylurea and sodium nitrite of 1000 and 100 mg/kg respectively inhibited DNA synthesis by 75%. With dimethylamine and sodium nitrite, a combination which results in gastric synthesis of dimethylnitrosamine, inhibitions of 65 and 57% were observed at 2000 mg/kg together with 150 mg/kg and 1000 mg/kg in combination with 100 mg/kg, respectively. In separate experiments, dimethylnitrosamine (50 mg/kg, p.o.) and diethylnitrosamine (100 mg/kg, i.p.) inhibited thymidine uptake by 30 and 89%, respectively. The mutagenic polynucelar hydrocarbon 3-methylcholanthrene (15 mg/kg) inhibited DNA synthesis by 95% and safrole (640 mg/kg), a mutagenic methylenedioxybenzene derivative, inhibited by 60%. Cadmium chloride (10 mg/kg), acetylaminofluorene (160 mg/kg) and dibutylnitrosamine (500 mg/kg) also induced statistically significant effects. Noncarcinogenic analogues of these substances (anthracene, 125 mg/kg; diphenyl-nitrosamine, 500 mg/kg; piperonyl butoxide, 640 mg/kg, and methylurea, 2000 mg/kg in combination with sodium nitrate) were inactive. Highly toxic substances (potassium cyanide, 2.5 mg/kg; 2,4-dinitrophenol, 20 mg/kg; and lead acetate, 150 mg/kg) were also inactive. This index of mutagenicity appears to have considerable sensitivity and therefore may have potential in drug evaluation.", "contents": "Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential sample mammalian assay for mutagenesis. Since mutagenic substances induce structural changes in DNA, studies were undertaken to determine whether substances would modify DNA replicative activity. DNA synthesis was quantitated 3.5 h after drug administration as uptake into DNA of a 30-min pulse of 10 muCi of [3H] thymidine. Combinations of methylurea (20000 mg/kg) and sodium nitrite (150 mg/kg) administered p.o. resulted in gastric synthesis of nitrosomethylurea and inhibited testicular DNA synthesis by 83%. Combinations of methylurea and sodium nitrite of 1000 and 100 mg/kg respectively inhibited DNA synthesis by 75%. With dimethylamine and sodium nitrite, a combination which results in gastric synthesis of dimethylnitrosamine, inhibitions of 65 and 57% were observed at 2000 mg/kg together with 150 mg/kg and 1000 mg/kg in combination with 100 mg/kg, respectively. In separate experiments, dimethylnitrosamine (50 mg/kg, p.o.) and diethylnitrosamine (100 mg/kg, i.p.) inhibited thymidine uptake by 30 and 89%, respectively. The mutagenic polynucelar hydrocarbon 3-methylcholanthrene (15 mg/kg) inhibited DNA synthesis by 95% and safrole (640 mg/kg), a mutagenic methylenedioxybenzene derivative, inhibited by 60%. Cadmium chloride (10 mg/kg), acetylaminofluorene (160 mg/kg) and dibutylnitrosamine (500 mg/kg) also induced statistically significant effects. Noncarcinogenic analogues of these substances (anthracene, 125 mg/kg; diphenyl-nitrosamine, 500 mg/kg; piperonyl butoxide, 640 mg/kg, and methylurea, 2000 mg/kg in combination with sodium nitrate) were inactive. Highly toxic substances (potassium cyanide, 2.5 mg/kg; 2,4-dinitrophenol, 20 mg/kg; and lead acetate, 150 mg/kg) were also inactive. This index of mutagenicity appears to have considerable sensitivity and therefore may have potential in drug evaluation.", "PMID": 967187}
{"id": "pubmed23n0032_6978", "title": "Cytogenetic effects of mutagens/carcinogens after activation in a microsomal system in vitro I. Induction of chromosome aberrations and sister chromatid exchanges by diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) in CHO cells in the presence of rat-liver microsomes.", "content": "A rat-liver microsomal system in vitro has been used to activate two indirectly acting carcinogens, DMN and DEN. On activation, both compounds were extremely potent in inducing chromosomal aberrations as well as sister chromatid exchanges in Chinese hamster cells. The implications of these findings and the potential utility of this technique to detect mutagens/carcinogens are discussed.", "contents": "Cytogenetic effects of mutagens/carcinogens after activation in a microsomal system in vitro I. Induction of chromosome aberrations and sister chromatid exchanges by diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) in CHO cells in the presence of rat-liver microsomes. A rat-liver microsomal system in vitro has been used to activate two indirectly acting carcinogens, DMN and DEN. On activation, both compounds were extremely potent in inducing chromosomal aberrations as well as sister chromatid exchanges in Chinese hamster cells. The implications of these findings and the potential utility of this technique to detect mutagens/carcinogens are discussed.", "PMID": 967188}
{"id": "pubmed23n0032_6979", "title": "Replicative bypass repair of ultraviolet damage to DNA of mammalian cells: caffeine sensitive and caffeine resistant mechanisms.", "content": "Replicative bypass repair of UV damage to DNA was studied in a wide variety of human, mouse and hamster cells in culture. Survival curve analysis revealed that in established cell lines (mouse L, Chinese hamster V79, HeLa S3 and SV40-transformed xeroderma pigmentosum (XP)), post-UV caffeine treatment potentiated cell killing by reducing the extrapolation number and mean lethal UV fluence (Do). In the Do reduction as the result of random inactivation by caffeine of sensitive repair there were marked clonal differences among such cell lines, V79 being most sensitive to caffeine potentiation. However, other diploid cell lines (normal human, excision-defective XP and Syrian hamster) exhibited no obvious reduction in Do by caffeine. In parallel, alkaline sucrose sedimentation results showed that the conversion of initially smaller segments of DNA synthesized after irradiation with 10 J/m2 to high-molecular-weight DNA was inhibited by caffeine in transformed XP cells, but not in the diploid human cell lines. Exceptionally, diploid XP variants had a retarded ability of bypass repair which was drastically prevented by caffeine, so that caffeine enhanced the lethal effect of UV. Neutral CsC1 study on the bypass repair mechanism by use of bromodeoxyuridine for DNA synthesis on damaged template suggests that the pyrimidine dimerer acts as a block to replication and subsequently it is circumvented presumably by a new process involving replicative bypassing following strand displacement, rather than by gap-filling de novo. This mechanism worked similarly in normal and XP cells, whether or not caffeine was present, indicating that excision of dimer is not always necessary. However, replicative bypassing become defective in XP variant and transformed XP cells when caffeine was present. It appears, therefore, that the replicative bypass repair process is either caffeine resistant or sensitive, depending on the cell type used, but not necessarily on the excision repair capability.", "contents": "Replicative bypass repair of ultraviolet damage to DNA of mammalian cells: caffeine sensitive and caffeine resistant mechanisms. Replicative bypass repair of UV damage to DNA was studied in a wide variety of human, mouse and hamster cells in culture. Survival curve analysis revealed that in established cell lines (mouse L, Chinese hamster V79, HeLa S3 and SV40-transformed xeroderma pigmentosum (XP)), post-UV caffeine treatment potentiated cell killing by reducing the extrapolation number and mean lethal UV fluence (Do). In the Do reduction as the result of random inactivation by caffeine of sensitive repair there were marked clonal differences among such cell lines, V79 being most sensitive to caffeine potentiation. However, other diploid cell lines (normal human, excision-defective XP and Syrian hamster) exhibited no obvious reduction in Do by caffeine. In parallel, alkaline sucrose sedimentation results showed that the conversion of initially smaller segments of DNA synthesized after irradiation with 10 J/m2 to high-molecular-weight DNA was inhibited by caffeine in transformed XP cells, but not in the diploid human cell lines. Exceptionally, diploid XP variants had a retarded ability of bypass repair which was drastically prevented by caffeine, so that caffeine enhanced the lethal effect of UV. Neutral CsC1 study on the bypass repair mechanism by use of bromodeoxyuridine for DNA synthesis on damaged template suggests that the pyrimidine dimerer acts as a block to replication and subsequently it is circumvented presumably by a new process involving replicative bypassing following strand displacement, rather than by gap-filling de novo. This mechanism worked similarly in normal and XP cells, whether or not caffeine was present, indicating that excision of dimer is not always necessary. However, replicative bypassing become defective in XP variant and transformed XP cells when caffeine was present. It appears, therefore, that the replicative bypass repair process is either caffeine resistant or sensitive, depending on the cell type used, but not necessarily on the excision repair capability.", "PMID": 967189}
{"id": "pubmed23n0032_6980", "title": "Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure.", "content": "To determine the relation between the presence of donor DNA polymerase and e antigen, and recipient hepatitis, we tested, under code, serums from a controlled trial of hepatitis B immune globulin used to treat individuals accidentally inoculated with HBs Ag-positive blood. All recipients lacked antibody to HBs Ag. In 29 of 31 donors, both polymerase and e were in perfect agreement; both demonstrated a highly significant correlation with recipient hepatitis (P less than 0.001). DNA polymerase/e-negative blood did not cause hepatitis. Blood containing polymerase or e antigen did not cause hepatitis in six of 31 and four of 18 recipients, respectively. Hepatitis did not correlate with transaminase or duration of antigenemia in the donor. Polymerase and e appear to be indicators of the relative infectivity of HBs Ag-positive serum, particularly after small-volume exposure. They may be important determinants in assessing infectivity of chronic carriers of HBs Ag and in evaluating efficacy of hepatitis B immune globulin and hepatitis B vaccines.", "contents": "Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure. To determine the relation between the presence of donor DNA polymerase and e antigen, and recipient hepatitis, we tested, under code, serums from a controlled trial of hepatitis B immune globulin used to treat individuals accidentally inoculated with HBs Ag-positive blood. All recipients lacked antibody to HBs Ag. In 29 of 31 donors, both polymerase and e were in perfect agreement; both demonstrated a highly significant correlation with recipient hepatitis (P less than 0.001). DNA polymerase/e-negative blood did not cause hepatitis. Blood containing polymerase or e antigen did not cause hepatitis in six of 31 and four of 18 recipients, respectively. Hepatitis did not correlate with transaminase or duration of antigenemia in the donor. Polymerase and e appear to be indicators of the relative infectivity of HBs Ag-positive serum, particularly after small-volume exposure. They may be important determinants in assessing infectivity of chronic carriers of HBs Ag and in evaluating efficacy of hepatitis B immune globulin and hepatitis B vaccines.", "PMID": 967200}
{"id": "pubmed23n0032_6981", "title": "Polycythemia vera: stem-cell and probable clonal origin of the disease.", "content": "Two women with polycythemia vera and heterozygosity (GdB/GdA) at the X-chromosome-linked locus for glucose-6-phosphate dehydrogenase were studied to determine the nature of the cellular origin of their polycythemia. In contrast to unaffected tissue, such as skin fibroblasts, which consisted of both B and A types, the glucose-6-phosphate dehydrogenase of the patients' erythrocytes, granulocytes and platelets was only of Type A. These results provide direct evidence for the stem-cell nature of polycythemia vera and strongly imply a clonal origin for this disease. The fact that no descendants of the presumed normal stem cells were found in circulation suggests that bone-marrow proliferation in this disorder is influenced by local (intramarrow) regulatory factors.", "contents": "Polycythemia vera: stem-cell and probable clonal origin of the disease. Two women with polycythemia vera and heterozygosity (GdB/GdA) at the X-chromosome-linked locus for glucose-6-phosphate dehydrogenase were studied to determine the nature of the cellular origin of their polycythemia. In contrast to unaffected tissue, such as skin fibroblasts, which consisted of both B and A types, the glucose-6-phosphate dehydrogenase of the patients' erythrocytes, granulocytes and platelets was only of Type A. These results provide direct evidence for the stem-cell nature of polycythemia vera and strongly imply a clonal origin for this disease. The fact that no descendants of the presumed normal stem cells were found in circulation suggests that bone-marrow proliferation in this disorder is influenced by local (intramarrow) regulatory factors.", "PMID": 967201}
{"id": "pubmed23n0032_6982", "title": "Doctors who perform operations. A study on in-hospital surgery in four diverse geographic areas (first of two parts).", "content": "The generous supply of surgeons in the United States stimulated a study of their operative work based on all operations performed by all physicians in hospitals of four geographic areas. Each operation was assigned a California Relative Value to permit work-load comparisons between specialties and practices. General practitioners constituted more than one quarter of physicians who performed operations but accounted for less than one tenth of total work. Surgical specialists, about half the physicians in the study, performed about 80 per cent of total operative work. Work loads of surgical specialists varied by certification, specialty, age and practice organization status. We conclude that far too many physicians perform surgical operations and that work loads of surgical specialists are modest. Calculations involving reallocation of operative work loads suggest that the total volume of operations in this study could have been handled by a substantially smaller cadre of busier surgical specialists.", "contents": "Doctors who perform operations. A study on in-hospital surgery in four diverse geographic areas (first of two parts). The generous supply of surgeons in the United States stimulated a study of their operative work based on all operations performed by all physicians in hospitals of four geographic areas. Each operation was assigned a California Relative Value to permit work-load comparisons between specialties and practices. General practitioners constituted more than one quarter of physicians who performed operations but accounted for less than one tenth of total work. Surgical specialists, about half the physicians in the study, performed about 80 per cent of total operative work. Work loads of surgical specialists varied by certification, specialty, age and practice organization status. We conclude that far too many physicians perform surgical operations and that work loads of surgical specialists are modest. Calculations involving reallocation of operative work loads suggest that the total volume of operations in this study could have been handled by a substantially smaller cadre of busier surgical specialists.", "PMID": 967202}
{"id": "pubmed23n0032_6983", "title": "Studies on the biological effects of deuteriated organic compounds. I. Antifungal activity of perdeuteriated fatty acids on dermatophytes in vitro.", "content": "The antifugal activity of some perdeuteriated fatty acids, with normal chain 11 to 18 carbon atoms, was investigated on common dermatophytes Epidermophyton floccosum, Microsporum canis, Trichophyton mentagrophytes and T. rubrum under in vitro conditions. These studies were performed by dilution technique and with respiratory measurements, Perdeuteriation of some fatty acids increases their inhibitory effect on the dermatophyte growth. Perdeuteriated n-hendecanoic acid proved to be the most active of the substances tested. Possible mechanisms behind the enhanced antifungal activity due to the perdeuteriation of fatty acids are discussed.", "contents": "Studies on the biological effects of deuteriated organic compounds. I. Antifungal activity of perdeuteriated fatty acids on dermatophytes in vitro. The antifugal activity of some perdeuteriated fatty acids, with normal chain 11 to 18 carbon atoms, was investigated on common dermatophytes Epidermophyton floccosum, Microsporum canis, Trichophyton mentagrophytes and T. rubrum under in vitro conditions. These studies were performed by dilution technique and with respiratory measurements, Perdeuteriation of some fatty acids increases their inhibitory effect on the dermatophyte growth. Perdeuteriated n-hendecanoic acid proved to be the most active of the substances tested. Possible mechanisms behind the enhanced antifungal activity due to the perdeuteriation of fatty acids are discussed.", "PMID": 967223}
{"id": "pubmed23n0032_6984", "title": "Studies on the biological effects of deuteriated organic compounds. II. Morphological changes of Epidermophyton floccosum exposed to perdeuteriated n-hendecanoic acid studied with interference contrast and scanning electron microscopy.", "content": "In order to visualise the morphological changes of Epidermophyton floccosum associated with exposure to perdeuteriated n-hendecanoic acid, the architecture of the dermatophyte was investigated by means of interference contrast and scanning electron microscopy. The morphology of mycelia grown on substrate containing perdeuteriated n-hendecanoic acid, or the unlabelled analogue, was compared. The perdeuteriated n-hendecanoic acid produced a characteristic undulant effect of the hyphae. The characteristic wave-like appearance of the mycelia looked similar to the curling effect occuring after treatment of dermatophytes with griseofulvin, but was not so pronounced. Perdeuteriated n-hendecanoic acid, unlike the unlabelled analogue, also seems to cause a reduction of the number of chlamydospores perforations of the macroconidia. The changes in the morphological structure of Epidermophyton floccosum exposed to pereuteriated n-hendecanoic acid have been investigated. Morphological examination of mycelia exposed to this substance by interference contrast microscopy demonstrated a picture of defect hyphae and macroconidia. By the aid of scanning electron microscopy we have attempted to obtain a better visualization of these changes at ultrastructural level.", "contents": "Studies on the biological effects of deuteriated organic compounds. II. Morphological changes of Epidermophyton floccosum exposed to perdeuteriated n-hendecanoic acid studied with interference contrast and scanning electron microscopy. In order to visualise the morphological changes of Epidermophyton floccosum associated with exposure to perdeuteriated n-hendecanoic acid, the architecture of the dermatophyte was investigated by means of interference contrast and scanning electron microscopy. The morphology of mycelia grown on substrate containing perdeuteriated n-hendecanoic acid, or the unlabelled analogue, was compared. The perdeuteriated n-hendecanoic acid produced a characteristic undulant effect of the hyphae. The characteristic wave-like appearance of the mycelia looked similar to the curling effect occuring after treatment of dermatophytes with griseofulvin, but was not so pronounced. Perdeuteriated n-hendecanoic acid, unlike the unlabelled analogue, also seems to cause a reduction of the number of chlamydospores perforations of the macroconidia. The changes in the morphological structure of Epidermophyton floccosum exposed to pereuteriated n-hendecanoic acid have been investigated. Morphological examination of mycelia exposed to this substance by interference contrast microscopy demonstrated a picture of defect hyphae and macroconidia. By the aid of scanning electron microscopy we have attempted to obtain a better visualization of these changes at ultrastructural level.", "PMID": 967224}
{"id": "pubmed23n0032_6985", "title": "Studies on the biological effects of deuteriated organic compounds. III. Antifungal activity of perdeuteriated fatty acids on dermatophytes in vivo experimental microsporie in guinea pigs.", "content": "Investigations on the antimycotic properties of perdeuteriated fatty acids were carried out on Microsporum cains infections in vivo. The study was performed on experimental microsporie in guinea pigs using four different methods, all based on the ability of M. canis to cause alopecia. Perdeuteriated n-hendecanoic acid showed in vivo a statistically significant enhanced antimycotic effect compared to its unlabelled analogue. This is in accordance with our previous observations in vitro conditions. The remaining perdeuteriated fatty acids (C12--C18) showed no statistically significant growth retarding effect on M. canis infections in guinea pigs when compared with their unlabelled analogues. The present study attempts to ascertain if some perdeuteriated fatty acids have any antifungal activity in vivo conditions. Our previous papers (3,4) concerning the evaluation of the antifungal activity of some perdeuteriated fatty acids on dermatophytes in vitro, demonstrated that the perdeuteriation of n-hendecanoic acid, lead to a pronounced antimycotic effect on common dermatophytes as e.g. E. floccosum, T. rubrum, M. canis and T. mentagrophytes. As our previous results indicate a decreasing of fungistasis with an increasing carbon-chain length of the perdeuteriated fatty acids, we have attempted to verify this observation in vivo conditions. A special interest was, of course, focused on the most promising compound, the perdeuteriated n-hendecanoic acid.", "contents": "Studies on the biological effects of deuteriated organic compounds. III. Antifungal activity of perdeuteriated fatty acids on dermatophytes in vivo experimental microsporie in guinea pigs. Investigations on the antimycotic properties of perdeuteriated fatty acids were carried out on Microsporum cains infections in vivo. The study was performed on experimental microsporie in guinea pigs using four different methods, all based on the ability of M. canis to cause alopecia. Perdeuteriated n-hendecanoic acid showed in vivo a statistically significant enhanced antimycotic effect compared to its unlabelled analogue. This is in accordance with our previous observations in vitro conditions. The remaining perdeuteriated fatty acids (C12--C18) showed no statistically significant growth retarding effect on M. canis infections in guinea pigs when compared with their unlabelled analogues. The present study attempts to ascertain if some perdeuteriated fatty acids have any antifungal activity in vivo conditions. Our previous papers (3,4) concerning the evaluation of the antifungal activity of some perdeuteriated fatty acids on dermatophytes in vitro, demonstrated that the perdeuteriation of n-hendecanoic acid, lead to a pronounced antimycotic effect on common dermatophytes as e.g. E. floccosum, T. rubrum, M. canis and T. mentagrophytes. As our previous results indicate a decreasing of fungistasis with an increasing carbon-chain length of the perdeuteriated fatty acids, we have attempted to verify this observation in vivo conditions. A special interest was, of course, focused on the most promising compound, the perdeuteriated n-hendecanoic acid.", "PMID": 967225}
{"id": "pubmed23n0032_6986", "title": "Kerion-like tinea capitis and barbae caused by Microsporum gypseum in Israel.", "content": "Four cases of hair invasion by Microsporum gypseum are reported. In three the scalp was involved and in one, the beard. All presented a kerion type of invasion. In 3 cases hair penetration was of the ectothrix type, while in one case the kerion of the scalp resembled the type of invasion seen in T. schonleini infection. None of the invaded hairs showed fluorescence in Wood's light. The soil was the source of infection in 3 patients, the fungus having been isolated from soil samples collected in the vicinity of the houses of these patients through the hair bait method. In one case the source of contamination was the family dog, in which scaly, alopecic, follicularlike lesions caused by the same agent were found.", "contents": "Kerion-like tinea capitis and barbae caused by Microsporum gypseum in Israel. Four cases of hair invasion by Microsporum gypseum are reported. In three the scalp was involved and in one, the beard. All presented a kerion type of invasion. In 3 cases hair penetration was of the ectothrix type, while in one case the kerion of the scalp resembled the type of invasion seen in T. schonleini infection. None of the invaded hairs showed fluorescence in Wood's light. The soil was the source of infection in 3 patients, the fungus having been isolated from soil samples collected in the vicinity of the houses of these patients through the hair bait method. In one case the source of contamination was the family dog, in which scaly, alopecic, follicularlike lesions caused by the same agent were found.", "PMID": 967226}
{"id": "pubmed23n0032_6987", "title": "Fungal degradation of anaerobically digested sewage sludge.", "content": "Fifty isolates of fungi were screened for their ability to grow on and degrade sludge. Cunninghamella elegans was selected for further study of degradation as affected by moisture, nutrients, time and temperature. Maximal sludge degradation (total dry weight basis) was 5.8% by Rhizopus oligosporus, 5.4% by C. elegans and 5.3% by Myrothecium verrucaria, representing approximately 11% degradation of the organic matter present. Added nutrients had little or no effect on sludge degradation. Maximal sludge degradation by C. elegans occurred in three weeks at 30-35 C or four weeks at 25 and 40 C.", "contents": "Fungal degradation of anaerobically digested sewage sludge. Fifty isolates of fungi were screened for their ability to grow on and degrade sludge. Cunninghamella elegans was selected for further study of degradation as affected by moisture, nutrients, time and temperature. Maximal sludge degradation (total dry weight basis) was 5.8% by Rhizopus oligosporus, 5.4% by C. elegans and 5.3% by Myrothecium verrucaria, representing approximately 11% degradation of the organic matter present. Added nutrients had little or no effect on sludge degradation. Maximal sludge degradation by C. elegans occurred in three weeks at 30-35 C or four weeks at 25 and 40 C.", "PMID": 967227}
{"id": "pubmed23n0032_6988", "title": "Miconazole in the treatment of superficial mycoses.", "content": "Miconazole nitrate (2%) cream was evaluated in the treatment of superficial mycoses. Out of 116 patients having multiple clinical diagnoses, 66 cases were found to be positive by culture. Species of Trichophyton were the predominant etiological agents (in over 60%) followed by Candida species (20%) and Epidermophyton floccosum (15%). All the cases selected for study were followed up to a period of 4-18 months. A cure rate of 94.6 per cent was observed in all the cases where causal organisms were isolated. Significantly high cure rate (66%) was also seen in cases where causal organisms could not be isolated, including cases of tinea versicolor. Results of mycological examination were in confirmity with the clinical results.", "contents": "Miconazole in the treatment of superficial mycoses. Miconazole nitrate (2%) cream was evaluated in the treatment of superficial mycoses. Out of 116 patients having multiple clinical diagnoses, 66 cases were found to be positive by culture. Species of Trichophyton were the predominant etiological agents (in over 60%) followed by Candida species (20%) and Epidermophyton floccosum (15%). All the cases selected for study were followed up to a period of 4-18 months. A cure rate of 94.6 per cent was observed in all the cases where causal organisms were isolated. Significantly high cure rate (66%) was also seen in cases where causal organisms could not be isolated, including cases of tinea versicolor. Results of mycological examination were in confirmity with the clinical results.", "PMID": 967228}
{"id": "pubmed23n0032_6989", "title": "Development of injectable microcapsules for use in the treatment of narcotic addiction.", "content": "Injectible microcapsules containing narcotic antagonists have been prepared with dl-poly (lactic acid) as the coating material. The encapsulation technology has developed to the point that high yields of less than 180 mu capsules can be prepared routinely. Such capsules with an initial payload of 50 wt. % naltrexone pamoate provide 60-90% antagonism to the action of morphine 28 days after injection into mice as a peanut oil/aluminum monostearate suspension at a dose level of 40 miligrams naltrexone pamoate/ kg. mouse.", "contents": "Development of injectable microcapsules for use in the treatment of narcotic addiction. Injectible microcapsules containing narcotic antagonists have been prepared with dl-poly (lactic acid) as the coating material. The encapsulation technology has developed to the point that high yields of less than 180 mu capsules can be prepared routinely. Such capsules with an initial payload of 50 wt. % naltrexone pamoate provide 60-90% antagonism to the action of morphine 28 days after injection into mice as a peanut oil/aluminum monostearate suspension at a dose level of 40 miligrams naltrexone pamoate/ kg. mouse.", "PMID": 967230}
{"id": "pubmed23n0032_6990", "title": "Long-acting narcotic antagonist complexes.", "content": "We evaluated the ability of close to 100 organic acids to form water-soluble salts with methadone, cyclazocine, naloxone, naltrexone and, more recently, diprenorphine. About half the acids yielded insoluble salts. Polybasic acids affording insoluble salts were evaluated for their ability to form drug:acid:metal complexes with the polyvalent metal ions, Zn++, Al+++, Mg++ and Ca++. Optimum conditions for forming complexes have been developed and the consistency of their composition has been established. Salts were analyzed spectrophotometrically for drug content, and complexes were analyzed for drug and metal content. The in vitro degree of dissociation at equilibrium was measured for the preparations suspended in a simulated physiological buffer, pH 7.3. Preparations of the narcotic antagonist drugs showing relatively low degrees of dissociation in vitro, since it early appeared that a high degree of dissociation contraindicated a prolonged duration of pharmacological action, were evaluated in mice after intramuscular administration at several dose levels by the mouse tail-flick test for the potency and duration of their morphine antagonist activity. Our most promising preparations to date, showing the most prolonged durations of action without evidence of gross toxicity, are naltrexone zinc tannate and naltrexone aluminum tannate. These are undergoing detailed evaluation as potential clinical candidates. Thus far, the most useful of several dosage forms studied is a suspension in an aluminum monostearate gel.", "contents": "Long-acting narcotic antagonist complexes. We evaluated the ability of close to 100 organic acids to form water-soluble salts with methadone, cyclazocine, naloxone, naltrexone and, more recently, diprenorphine. About half the acids yielded insoluble salts. Polybasic acids affording insoluble salts were evaluated for their ability to form drug:acid:metal complexes with the polyvalent metal ions, Zn++, Al+++, Mg++ and Ca++. Optimum conditions for forming complexes have been developed and the consistency of their composition has been established. Salts were analyzed spectrophotometrically for drug content, and complexes were analyzed for drug and metal content. The in vitro degree of dissociation at equilibrium was measured for the preparations suspended in a simulated physiological buffer, pH 7.3. Preparations of the narcotic antagonist drugs showing relatively low degrees of dissociation in vitro, since it early appeared that a high degree of dissociation contraindicated a prolonged duration of pharmacological action, were evaluated in mice after intramuscular administration at several dose levels by the mouse tail-flick test for the potency and duration of their morphine antagonist activity. Our most promising preparations to date, showing the most prolonged durations of action without evidence of gross toxicity, are naltrexone zinc tannate and naltrexone aluminum tannate. These are undergoing detailed evaluation as potential clinical candidates. Thus far, the most useful of several dosage forms studied is a suspension in an aluminum monostearate gel.", "PMID": 967231}
{"id": "pubmed23n0032_6991", "title": "Sustained release of naltrexone from glyceride implants.", "content": "Solid dispersions of naltrexone in natural glycerides were used to form injectable implants which continuously release narcotic antagonists in vivo. The dispersions were formed and tested either as small cylindrical pellets, e.g. l x 3.0 mm in size, or as particles with diameters in size ranges between 125-250 mu, that are suspended in an aqueous methyl cellulose solution. Both types of implants delivered naltrexone to mice at rates that were effective in blocking the antiociceptive action of morphine for at least one month. The rate of naltrexone release was controlled by altering its concentration in the dispersion and by varying the glyceride composition. Degradation and absorption of the implants were found to depend on their composition, dimensions and location in the body. No appreciable tissue incompatibility was seen in mice, rats, rabbits, monkeys and swine, even when long-lasting preparations were removed a year after treatment.", "contents": "Sustained release of naltrexone from glyceride implants. Solid dispersions of naltrexone in natural glycerides were used to form injectable implants which continuously release narcotic antagonists in vivo. The dispersions were formed and tested either as small cylindrical pellets, e.g. l x 3.0 mm in size, or as particles with diameters in size ranges between 125-250 mu, that are suspended in an aqueous methyl cellulose solution. Both types of implants delivered naltrexone to mice at rates that were effective in blocking the antiociceptive action of morphine for at least one month. The rate of naltrexone release was controlled by altering its concentration in the dispersion and by varying the glyceride composition. Degradation and absorption of the implants were found to depend on their composition, dimensions and location in the body. No appreciable tissue incompatibility was seen in mice, rats, rabbits, monkeys and swine, even when long-lasting preparations were removed a year after treatment.", "PMID": 967232}
{"id": "pubmed23n0032_6992", "title": "Use of synthetic polypeptides in the preparation of biodegradable delivery vehicles for narcotic antagonists.", "content": "Synthetic polypeptides consisting of copolymers of glutamic acid and leucine have been shown to be useful materials for the fabrication of practical, biodegradable delivery vehicles for narcotic antagonists. Model delivery vehicles in film form were prepared from copolymers containing 10 mole percent to 40 mole percent glutamic acid, and loaded with 10% to 40% naltrexone by weight. The naltrexone was found to be released by diffusion, exhibiting diffusion coefficients that varied as a function of the glutamic acid content and the initial naltrexone loading. A wide range in diffusion coefficients were achieved (0.31 x 10-7 cm2/hr to 120 x 10-7 cm2/hr), leading to release rates within practical ranges of interest for meeting the program goals. We have demonstrated that the polypeptides can be fabricated into dosage forms that are amenable to administration by trochar. For example, rods 0.4 mm to 0.8 mm in diameter containing as much as 40% naltrexone by weight were extruded using a simple compression mold and die arrangement. An in vitro evaluation of the rods showed that antagonist is released by diffusion at a continuously decreasing rate, a behavior similar to that observed with the film devices that were, nonetheless, capable of blocking an AD80 challenge of morphene sulfate in mice for more than 30 days. One of the most promising delivery vehicles that we have developed to date consists of a polypeptide tube filled with a naltrexone/polypeptide core. Preliminary experiments have shown that these devices may be capable of administering high, constant rates of release for prolonged periods of time. Additional work, however, is required to develop techniques for the preparation of reproducible delivery vehicles.", "contents": "Use of synthetic polypeptides in the preparation of biodegradable delivery vehicles for narcotic antagonists. Synthetic polypeptides consisting of copolymers of glutamic acid and leucine have been shown to be useful materials for the fabrication of practical, biodegradable delivery vehicles for narcotic antagonists. Model delivery vehicles in film form were prepared from copolymers containing 10 mole percent to 40 mole percent glutamic acid, and loaded with 10% to 40% naltrexone by weight. The naltrexone was found to be released by diffusion, exhibiting diffusion coefficients that varied as a function of the glutamic acid content and the initial naltrexone loading. A wide range in diffusion coefficients were achieved (0.31 x 10-7 cm2/hr to 120 x 10-7 cm2/hr), leading to release rates within practical ranges of interest for meeting the program goals. We have demonstrated that the polypeptides can be fabricated into dosage forms that are amenable to administration by trochar. For example, rods 0.4 mm to 0.8 mm in diameter containing as much as 40% naltrexone by weight were extruded using a simple compression mold and die arrangement. An in vitro evaluation of the rods showed that antagonist is released by diffusion at a continuously decreasing rate, a behavior similar to that observed with the film devices that were, nonetheless, capable of blocking an AD80 challenge of morphene sulfate in mice for more than 30 days. One of the most promising delivery vehicles that we have developed to date consists of a polypeptide tube filled with a naltrexone/polypeptide core. Preliminary experiments have shown that these devices may be capable of administering high, constant rates of release for prolonged periods of time. Additional work, however, is required to develop techniques for the preparation of reproducible delivery vehicles.", "PMID": 967233}
{"id": "pubmed23n0032_6993", "title": "Development of Chronomers tm for narcotic antagonists.", "content": "The object of this program is to prepare a bioerodable naltrexone delivery system which can be implanted subcutaneously in humans and which can relieve the narcotic antagonist over 1-6 months at relatively constant and sufficient rates to block the euphoric effect of morphine based drugs. The system is composed of naltrexone uniformly dispered in a solid hydropholic CHRONOMER TM matrix which undergoes predictable surface erosion when exposed to an aqueous medium. Kinetic studies in vitro have been carried out during the course of the program to determine the best composition for the system. Toxilogical studies conducted at ALZA during the past 2 years have not revealed limiting adverse effects of either the CHRONOMER TM materials or their hydrolysis products. The tail-flick test procedure was used to measure the effectiveness of naltrexone to antagonize the analgesis of morphine in rats. Naltrexone infused intravenously at doses of 4 and 16 ug/kg/hr resulted in, after 6 hours, 54 and 89% antagonism, respectively, against a 63.5% effective dose of morphine. Perliminary sterilization studies showed that no adverse effects to CHRONOMER TM/naltrexone systems occurred after exposure to 2.5 or 5.0 mrads of 60CO irradiation.", "contents": "Development of Chronomers tm for narcotic antagonists. The object of this program is to prepare a bioerodable naltrexone delivery system which can be implanted subcutaneously in humans and which can relieve the narcotic antagonist over 1-6 months at relatively constant and sufficient rates to block the euphoric effect of morphine based drugs. The system is composed of naltrexone uniformly dispered in a solid hydropholic CHRONOMER TM matrix which undergoes predictable surface erosion when exposed to an aqueous medium. Kinetic studies in vitro have been carried out during the course of the program to determine the best composition for the system. Toxilogical studies conducted at ALZA during the past 2 years have not revealed limiting adverse effects of either the CHRONOMER TM materials or their hydrolysis products. The tail-flick test procedure was used to measure the effectiveness of naltrexone to antagonize the analgesis of morphine in rats. Naltrexone infused intravenously at doses of 4 and 16 ug/kg/hr resulted in, after 6 hours, 54 and 89% antagonism, respectively, against a 63.5% effective dose of morphine. Perliminary sterilization studies showed that no adverse effects to CHRONOMER TM/naltrexone systems occurred after exposure to 2.5 or 5.0 mrads of 60CO irradiation.", "PMID": 967234}
{"id": "pubmed23n0032_6994", "title": "Analytical methods for the determination of cannabinoids in biological media.", "content": "A pharmacokinetic study of the blood plasma levels in man of delta9-tetrahydrocannabinol, 11-hydroxy-delta9-tetrahydrocannabinol and cannabinol has been carried out by means of combined gas chromatographic-mass spectral analysis. In some cases comparison of the data was obtained on the same sample using thin layer chromatography of radiolabeled samples and electron capture gas-liquid chromatography. For the mass spectral studies appropriately deuterium labeled analogs of the previously named compounds were used both as internal standards and as a carrier for the relatively small amounts of nonlabeled drug present in plasma. Blood samples were obtainted at periodic intervals up to 24 hours from volunteers receiving 4-5 mg delta9-THC intravenously. After extraction and \"clean-up\" by Sephadex chromatography, the extracts were concentrated and subjected to glc-ms in the electron impact (ei) mode or alternatively with a chemical ionization (ci) source, in which case preliminary chromatography could be omitted. In all cases calibration curves were obtained from replicate analyses of spiked plasma containing the internal standard and various quantities of the cannabinoid under analysis. A typical biphasic elimination of the drug was observed with rapid elimination of delta9-THC from the blood over a period of 40 min followed by a much slower elimination up to 24 hours. The experimental data show that 11-hydroxy-delta9-THC is found in the plasma in quantities only about one-twentieth to one-twenty-fifth the values found for delta9-THC. Cannabinol was not found in significant quantities. Good agreement was obtained between the mass spectral analyses and the thin layer chromatography or electron capture gas-liquid chromatographic procedures.", "contents": "Analytical methods for the determination of cannabinoids in biological media. A pharmacokinetic study of the blood plasma levels in man of delta9-tetrahydrocannabinol, 11-hydroxy-delta9-tetrahydrocannabinol and cannabinol has been carried out by means of combined gas chromatographic-mass spectral analysis. In some cases comparison of the data was obtained on the same sample using thin layer chromatography of radiolabeled samples and electron capture gas-liquid chromatography. For the mass spectral studies appropriately deuterium labeled analogs of the previously named compounds were used both as internal standards and as a carrier for the relatively small amounts of nonlabeled drug present in plasma. Blood samples were obtainted at periodic intervals up to 24 hours from volunteers receiving 4-5 mg delta9-THC intravenously. After extraction and \"clean-up\" by Sephadex chromatography, the extracts were concentrated and subjected to glc-ms in the electron impact (ei) mode or alternatively with a chemical ionization (ci) source, in which case preliminary chromatography could be omitted. In all cases calibration curves were obtained from replicate analyses of spiked plasma containing the internal standard and various quantities of the cannabinoid under analysis. A typical biphasic elimination of the drug was observed with rapid elimination of delta9-THC from the blood over a period of 40 min followed by a much slower elimination up to 24 hours. The experimental data show that 11-hydroxy-delta9-THC is found in the plasma in quantities only about one-twentieth to one-twenty-fifth the values found for delta9-THC. Cannabinol was not found in significant quantities. Good agreement was obtained between the mass spectral analyses and the thin layer chromatography or electron capture gas-liquid chromatographic procedures.", "PMID": 967238}
{"id": "pubmed23n0032_6995", "title": "Separation and sensitive assay of THC in biological fluids by HPLC and GLC.", "content": "HPLC systems were developed to permit quantitative separation of delta9-tetrahydrocannabinol from many of the heptane extractable lipoidal and other endogenous substances in biological fluids. These substances interfered with the quantification by flame ionization GLC of unmodified compound and by electron capture GLC of pentafluorobenzoylated compound. Reverse phase HPLC elution, with 47% acetonitrile in water, and normal phase HPLC with 25% chloroform in heptane, separated tetrahydrocannabinol from 11-hydroxy-delta9-tetrahydrocannabinol and other monohydroxylated tetrahydrocannabinols. These systems also purified stock solutions of tetrahydrocannabinol from accompanying contaminants. The various monohydroxylated tetrahydrocannabinols were resolved from each other in normal phase, 80% chloroform in heptane. The delta8 and delta9-tetrahydrocannabinols were separable in normal phase with 5% tetrahydrofuran in hexane. The GLC analysis of pentafluorobenzoylated tetrahydrocannabinol had a sensitivity of 1 ng/ml of plasma with an estimated 5% standard error of an assay with the extraction and GLC procedures given herein. Radiochemical analysis of the HPLC separated fraction had s sensitivity of 0.2 ng/ml of plasma with an estimated 2% standard error of an assay. There was no significant difference between the liquid scintillation and electron capture GLC assays of the HPLC separated delta9-tetrahydrocannabinol obtained from the plasma of dogs administered the drug. Radiolabelled compounds can be added to plasma samples as internal standards to determine the recovery efficiencies of the several procedures in the analysis of unlabelled tetrahydrocannabinol.", "contents": "Separation and sensitive assay of THC in biological fluids by HPLC and GLC. HPLC systems were developed to permit quantitative separation of delta9-tetrahydrocannabinol from many of the heptane extractable lipoidal and other endogenous substances in biological fluids. These substances interfered with the quantification by flame ionization GLC of unmodified compound and by electron capture GLC of pentafluorobenzoylated compound. Reverse phase HPLC elution, with 47% acetonitrile in water, and normal phase HPLC with 25% chloroform in heptane, separated tetrahydrocannabinol from 11-hydroxy-delta9-tetrahydrocannabinol and other monohydroxylated tetrahydrocannabinols. These systems also purified stock solutions of tetrahydrocannabinol from accompanying contaminants. The various monohydroxylated tetrahydrocannabinols were resolved from each other in normal phase, 80% chloroform in heptane. The delta8 and delta9-tetrahydrocannabinols were separable in normal phase with 5% tetrahydrofuran in hexane. The GLC analysis of pentafluorobenzoylated tetrahydrocannabinol had a sensitivity of 1 ng/ml of plasma with an estimated 5% standard error of an assay with the extraction and GLC procedures given herein. Radiochemical analysis of the HPLC separated fraction had s sensitivity of 0.2 ng/ml of plasma with an estimated 2% standard error of an assay. There was no significant difference between the liquid scintillation and electron capture GLC assays of the HPLC separated delta9-tetrahydrocannabinol obtained from the plasma of dogs administered the drug. Radiolabelled compounds can be added to plasma samples as internal standards to determine the recovery efficiencies of the several procedures in the analysis of unlabelled tetrahydrocannabinol.", "PMID": 967239}
{"id": "pubmed23n0032_6996", "title": "Rolling hairpin model for replication of parvovirus and linear chromosomal DNA.", "content": "A novel, quasicircular scheme is proposed for the replication of parvovirus DNA. Daughter strands are initiated after the copying and rearrangement of a terminal palindromic sequence, a process termed 'hairpin transfer'. Such a process may be involved in the replication of other viruses and host cell DNA.", "contents": "Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. A novel, quasicircular scheme is proposed for the replication of parvovirus DNA. Daughter strands are initiated after the copying and rearrangement of a terminal palindromic sequence, a process termed 'hairpin transfer'. Such a process may be involved in the replication of other viruses and host cell DNA.", "PMID": 967244}
{"id": "pubmed23n0032_6997", "title": "[Atmospheric trace chemicals].", "content": "This paper deals with the important trace-materials in the natural and anthropogenetic influenced atmosphere. They exist as gases as well as solid and solid-fluid particles. They are significant for numerous physical processes in the atmosphere and have also a direct influence on our biological environment.", "contents": "[Atmospheric trace chemicals]. This paper deals with the important trace-materials in the natural and anthropogenetic influenced atmosphere. They exist as gases as well as solid and solid-fluid particles. They are significant for numerous physical processes in the atmosphere and have also a direct influence on our biological environment.", "PMID": 967259}
{"id": "pubmed23n0032_6998", "title": "Atmospheric ozone and man-made pollution.", "content": "Atmospheric photochemistry and transport processes, related to the ozone layer, are discussed. Natural or man-made changes of the biosphere, variations of radiation, or general circulation as well as anthropogenic release of ozone-destroying catalysts are likely to alter the earth's ozone shield. The possible effects of ozone depletion caused by supersonic aircraft, nuclear weapons, nitrogen fertilizers, and chlorofluoromethanes are discussed.", "contents": "Atmospheric ozone and man-made pollution. Atmospheric photochemistry and transport processes, related to the ozone layer, are discussed. Natural or man-made changes of the biosphere, variations of radiation, or general circulation as well as anthropogenic release of ozone-destroying catalysts are likely to alter the earth's ozone shield. The possible effects of ozone depletion caused by supersonic aircraft, nuclear weapons, nitrogen fertilizers, and chlorofluoromethanes are discussed.", "PMID": 967263}
{"id": "pubmed23n0032_6999", "title": "Progress in biochemical engineering.", "content": "Biochemical engineering is one of the answers to some of the challenges of the present age: hunger, shortage in raw material and energy supply and contamination of environment. Its contribution to the solution of these problems is the industrial production of protein, the use of raw materials (incl. waste products) not used up to now, the accomplishment of chemical reactions at ambient temperatures as well as the degradation or utilization of widely different waste materials.", "contents": "Progress in biochemical engineering. Biochemical engineering is one of the answers to some of the challenges of the present age: hunger, shortage in raw material and energy supply and contamination of environment. Its contribution to the solution of these problems is the industrial production of protein, the use of raw materials (incl. waste products) not used up to now, the accomplishment of chemical reactions at ambient temperatures as well as the degradation or utilization of widely different waste materials.", "PMID": 967269}
{"id": "pubmed23n0032_7000", "title": "Evidence for a free-running circadian rhythm in pituitary-adrenal function in blinded adult female rats.", "content": "To study the eyes' role in maintaining the circadian rhythm in pituitary-adrenal function, 24-h patterns of corticosterone levels were compared in intact and blinded adult female rats. Rats were blinded by optic enucleation at approximately 80 days of age. Nonstress plasma corticosterone levels were determined fluorometrically in serial blood samples obtained from a tail vein at 4-h intervals for 44-h periods, 3 and 10 weeks after surgery. At 3 weeks after surgery, blinded and intact rats demonstrated comparable rhythms in corticosterone levels. At 10 weeks, steroid fluctuations in individual blinded rats still had an approximate 24-h periodicity. However, these fluctuations were no longer synchronized with the light-dark cycle or with those of other rats. These findings suggest that rats blinded as adults have a free-running pituitary-adrenal circadian rhythm.", "contents": "Evidence for a free-running circadian rhythm in pituitary-adrenal function in blinded adult female rats. To study the eyes' role in maintaining the circadian rhythm in pituitary-adrenal function, 24-h patterns of corticosterone levels were compared in intact and blinded adult female rats. Rats were blinded by optic enucleation at approximately 80 days of age. Nonstress plasma corticosterone levels were determined fluorometrically in serial blood samples obtained from a tail vein at 4-h intervals for 44-h periods, 3 and 10 weeks after surgery. At 3 weeks after surgery, blinded and intact rats demonstrated comparable rhythms in corticosterone levels. At 10 weeks, steroid fluctuations in individual blinded rats still had an approximate 24-h periodicity. However, these fluctuations were no longer synchronized with the light-dark cycle or with those of other rats. These findings suggest that rats blinded as adults have a free-running pituitary-adrenal circadian rhythm.", "PMID": 967300}
{"id": "pubmed23n0032_7001", "title": "Effect of variations in pituitary-adrenal activity on dopamine-beta-hydroxylase activity in various regions of rat brain.", "content": "Since noradrenergic neurons in the brain appear to inhibit ACTH secretion and dopamine-beta-hydroxylase (DBH) is found in noradrenergic neurons, the effect of variations in pituitary-adrenal activity on the activity of DBH in the brain stem, hypothalamus and hippocampus was determined. There was no significant circadian fluctuation in hypothalamic or brain stem DBH, as measured by the coupled radioenzymatic method of MOLINOFF et. al. [1971]. Pentobarbital and ether anesthesia, injection stress and surgical stress also had no acute effect. There was a decrease in anterior hypothalamic DBH 30 min after immobilization stress. Two days after adrenalectomy, there was a decrease in DBH in the hypothalamus and brain stem. A large dose of corticosterone (B) caused an increase in hypothalamic DBH. However, a smaller dose of B which increased plasma B to values comparable to those produced by endogenous secretion failed to have this effect. The data demonstrate an effect of the adrenal glands on brain DBH activity, but it is as yet uncertain whether this effect is mediated by glucocorticoids.", "contents": "Effect of variations in pituitary-adrenal activity on dopamine-beta-hydroxylase activity in various regions of rat brain. Since noradrenergic neurons in the brain appear to inhibit ACTH secretion and dopamine-beta-hydroxylase (DBH) is found in noradrenergic neurons, the effect of variations in pituitary-adrenal activity on the activity of DBH in the brain stem, hypothalamus and hippocampus was determined. There was no significant circadian fluctuation in hypothalamic or brain stem DBH, as measured by the coupled radioenzymatic method of MOLINOFF et. al. [1971]. Pentobarbital and ether anesthesia, injection stress and surgical stress also had no acute effect. There was a decrease in anterior hypothalamic DBH 30 min after immobilization stress. Two days after adrenalectomy, there was a decrease in DBH in the hypothalamus and brain stem. A large dose of corticosterone (B) caused an increase in hypothalamic DBH. However, a smaller dose of B which increased plasma B to values comparable to those produced by endogenous secretion failed to have this effect. The data demonstrate an effect of the adrenal glands on brain DBH activity, but it is as yet uncertain whether this effect is mediated by glucocorticoids.", "PMID": 967301}
{"id": "pubmed23n0032_7002", "title": "Hypophysectomy facilitates sexual behavior in female rats.", "content": "Lordosis was elecited in 49% of 87 hormonally untreated, hypophysectomized-ovariectomized (hypox-ovx) female rats in response to palpation of the flanks and perineum (vaginal stimulation was not applied). By contrast, only 12% of 113 hormonally untreated ovariectomized (ovx) rats showed lordosis in response to such stimulation. Subsequently, hypox-ovx and ovx-only rats were given daily injections of 1 mug/kg estradiol benzoate (EB) and tested for sexual receptivity with males. Teh estrogen-treated hypox-ovx females became sexually receptive significantly earlier, and exhibited higher lordosis quotients and more soliciting behavior, than the estrogen-treated ovx-only rats. The increased sexual responsiveness in the hypox-ovx rats could be due to increased LRH activity. To test this, we treated hypox-ovx rats with dihydrotestosterone propionate (DHT-P), which suppresses plasma LH levels but is relatively ineffective in inducing sexual receptivity, and found a significant depression of lordosis responsiveness. These experiments suggest that hypox-ovx females show a heightened responsiveness to hormonal and/or sensory factors that induce a lordosis response, possibly because of increased LRH activity.", "contents": "Hypophysectomy facilitates sexual behavior in female rats. Lordosis was elecited in 49% of 87 hormonally untreated, hypophysectomized-ovariectomized (hypox-ovx) female rats in response to palpation of the flanks and perineum (vaginal stimulation was not applied). By contrast, only 12% of 113 hormonally untreated ovariectomized (ovx) rats showed lordosis in response to such stimulation. Subsequently, hypox-ovx and ovx-only rats were given daily injections of 1 mug/kg estradiol benzoate (EB) and tested for sexual receptivity with males. Teh estrogen-treated hypox-ovx females became sexually receptive significantly earlier, and exhibited higher lordosis quotients and more soliciting behavior, than the estrogen-treated ovx-only rats. The increased sexual responsiveness in the hypox-ovx rats could be due to increased LRH activity. To test this, we treated hypox-ovx rats with dihydrotestosterone propionate (DHT-P), which suppresses plasma LH levels but is relatively ineffective in inducing sexual receptivity, and found a significant depression of lordosis responsiveness. These experiments suggest that hypox-ovx females show a heightened responsiveness to hormonal and/or sensory factors that induce a lordosis response, possibly because of increased LRH activity.", "PMID": 967302}
{"id": "pubmed23n0032_7003", "title": "Immobilization does not inhibit the post-coital ovulatory surge of luteinizing hormone in the rabbit.", "content": "Heparinized blood samples were obtained through an indwelling catheter in the central ear artery from sexually mature female New Zealand white rabbits after copulation. Immediately after 1 or 2 matings the female was transferred to a restraining cage; blood samples were taken at intervals while the animal was completely immobilized for 3-5 h or was only immobilized for blood withdrawal. Ovulation was detected by the presence of corpora lutea at laparotomy the following day. Plasma was analyzed for LH by established radioimmunoassay procedures. Immobilization did not inhibit ovulation or the pre-ovulatory surge of LH in either group. These data suggest that immobilization cannot inhibit the reflex release of LH in the female rabbit.", "contents": "Immobilization does not inhibit the post-coital ovulatory surge of luteinizing hormone in the rabbit. Heparinized blood samples were obtained through an indwelling catheter in the central ear artery from sexually mature female New Zealand white rabbits after copulation. Immediately after 1 or 2 matings the female was transferred to a restraining cage; blood samples were taken at intervals while the animal was completely immobilized for 3-5 h or was only immobilized for blood withdrawal. Ovulation was detected by the presence of corpora lutea at laparotomy the following day. Plasma was analyzed for LH by established radioimmunoassay procedures. Immobilization did not inhibit ovulation or the pre-ovulatory surge of LH in either group. These data suggest that immobilization cannot inhibit the reflex release of LH in the female rabbit.", "PMID": 967303}
{"id": "pubmed23n0032_7004", "title": "Cytoplasmic and nuclear metabolism of testosterone in the brains of neonatal and prepubertal rats.", "content": "The metabolism of testosterone (T) in neonatal rat brains was measured following in vivo and in vitro incubations with 3H-testosterone.Steroids associated with nuclear and cytoplasmic fractions of brain tissue from 4, 12, and 32-day-old male and female rats were identified. Although there are quantitative differences under different methods of incubation, in all cases more than 95% of the radioactivity was recovered and identified as T, or the 5alpha-reduced metabolities, DHT and 3alpha-androstanediol. Most of the metabolism of T occurred in the cytoplasm but 5alpha-reduced metabolities were also associated with purified nuclei. Reaction kinetics indicate that the metabolic sequence is T leads to DHT leads to 3alpha DIOL. DHT levels were similar in the cytoplasmic fractions from the neonates (4 and 12-day-old animals) and prepubertal animals (32-day-old animals), but 3alpha-androstanediol was significantly reduced in these fractions from prepubertal animals compared to those from neonates. Sex differences in the metabolism of T in the various subcellular fractions were not detectable.", "contents": "Cytoplasmic and nuclear metabolism of testosterone in the brains of neonatal and prepubertal rats. The metabolism of testosterone (T) in neonatal rat brains was measured following in vivo and in vitro incubations with 3H-testosterone.Steroids associated with nuclear and cytoplasmic fractions of brain tissue from 4, 12, and 32-day-old male and female rats were identified. Although there are quantitative differences under different methods of incubation, in all cases more than 95% of the radioactivity was recovered and identified as T, or the 5alpha-reduced metabolities, DHT and 3alpha-androstanediol. Most of the metabolism of T occurred in the cytoplasm but 5alpha-reduced metabolities were also associated with purified nuclei. Reaction kinetics indicate that the metabolic sequence is T leads to DHT leads to 3alpha DIOL. DHT levels were similar in the cytoplasmic fractions from the neonates (4 and 12-day-old animals) and prepubertal animals (32-day-old animals), but 3alpha-androstanediol was significantly reduced in these fractions from prepubertal animals compared to those from neonates. Sex differences in the metabolism of T in the various subcellular fractions were not detectable.", "PMID": 967304}
{"id": "pubmed23n0032_7005", "title": "[Acute plasma-cell leukemia. Report of a case].", "content": "A case of fatal, diffused plasmacytoma is presented. The picture also included leukaemia and is felt to be an example of plasma-cell leukaemia, as opposed to plasmacytoma in the light of the anatomical and clinical features of the onset and course: high and very pronounced plasma-cell leukocytosis, diffuse proliferation of the plasma cell series in the bone marrow, spleen and lymph nodes, infiltration of plasma cells in the parenchymal organs, extremely rapid progress with serious anaemia, haemorrhage, general organic involvement, high fever, and infection.", "contents": "[Acute plasma-cell leukemia. Report of a case]. A case of fatal, diffused plasmacytoma is presented. The picture also included leukaemia and is felt to be an example of plasma-cell leukaemia, as opposed to plasmacytoma in the light of the anatomical and clinical features of the onset and course: high and very pronounced plasma-cell leukocytosis, diffuse proliferation of the plasma cell series in the bone marrow, spleen and lymph nodes, infiltration of plasma cells in the parenchymal organs, extremely rapid progress with serious anaemia, haemorrhage, general organic involvement, high fever, and infection.", "PMID": 967335}
{"id": "pubmed23n0032_7006", "title": "[Clinical applicability of an enzyme micromethod for the rapid determination of blood sugar].", "content": "The clinical applicability of an enzymatic micromethod for the fast determination of glycaemia is discussed. The method is based on the use of an optical reflectometer for the quantitative reading of the variation in colour intensity of specific reactive strips for the semi-quantitative evaluation of blood glucose. Thanks to the method's rapidity and simplicity, it can be used for mass screening and is also very useful for the routine investigations of Diabetologic Centres. It is also invaluable for the identification of emergency clinical situations.", "contents": "[Clinical applicability of an enzyme micromethod for the rapid determination of blood sugar]. The clinical applicability of an enzymatic micromethod for the fast determination of glycaemia is discussed. The method is based on the use of an optical reflectometer for the quantitative reading of the variation in colour intensity of specific reactive strips for the semi-quantitative evaluation of blood glucose. Thanks to the method's rapidity and simplicity, it can be used for mass screening and is also very useful for the routine investigations of Diabetologic Centres. It is also invaluable for the identification of emergency clinical situations.", "PMID": 967336}
{"id": "pubmed23n0032_7007", "title": "[Medical treatment of arrhythmias in myocardial infarct. Results of the use of bunaftine].", "content": "After a short survey of the physiopathology of arrhythmia in myocardial infarction, especially at the initial stage, the results of using bunaphthine in 21 personal cases of myocardial infarction are reported and the fully satisfactory results described. This substance is held to be an effective, useful antiarrhythmia drug which can be usefully employed in the prophylaxis of arrhythmia in myocardial infarction.", "contents": "[Medical treatment of arrhythmias in myocardial infarct. Results of the use of bunaftine]. After a short survey of the physiopathology of arrhythmia in myocardial infarction, especially at the initial stage, the results of using bunaphthine in 21 personal cases of myocardial infarction are reported and the fully satisfactory results described. This substance is held to be an effective, useful antiarrhythmia drug which can be usefully employed in the prophylaxis of arrhythmia in myocardial infarction.", "PMID": 967338}
{"id": "pubmed23n0032_7008", "title": "[On a syndrome of immunopathic type with pleural and systemic localization observed in patients with intracavitary electrostimulators. Considerations on 4 cases].", "content": "The complications arising out of the installation of pacemakers are listed and classified and it is suggested that a syndrome characterized by recurrent pleural effusion recalcitrant to the usual medico-depletive therapies, observed in four patients with pacemakers, should be included among such complications. The clinical pictures of the syndrome is described in detail and particular attention is paid to its date of onset in relation to installation of the pacemaker, early symptomatology, the most typical symptoms, clinico-humoral development, prognosis and therapy. Some theoretical and practical remarks are also offered, specifically three pathogenetic suggestions are formulated on the basis of clinical and developmental features but mainly in relation to humoral and immunological findings. These suggestions stress alternative interpretations of the syndrome: A) as an autoimmune disease arising secondary to surgical denaturation of myocardial and/or endocardial autoantigens; B) as a systemic autoimmune disease due to congenital or acquired insufficiency of immunological homeostasis; C) as a monosymptomatic pleural form of systemic lupus erythematodes with late clinical outbreak. The fascinating pathogenetic, clinical, biological and therapeutic resemblances between the present syndrome and the post-infarctual syndrome of Dressler and Johnson's post-pericardiotomic syndrome are pointed out and it is suggested that complications of medical nature already described as being secondary to the installation of pacemakers, such as endocarditis and pericarditis, should be looked at from an autoimmune type of pathogenetic viewpoint. Finally, in the light of personal observations, the need for immunological study of high risk patients, namely relations of patients with autoimmune diseases, is stressed. All candidates for pacemaker installation should also be included. Routine screening of this type would take on enormous practical importance as it would enable preventive or curative treatment to be undertaken to modify the immunitary balance of those subjects in whom pacemaker installation is liable to trigger off critical, irreversible breakdown in immunitary homoeostasis of which the syndrome in question would seem to be the most dramatic, but perhaps no the only, clinical manifestation.", "contents": "[On a syndrome of immunopathic type with pleural and systemic localization observed in patients with intracavitary electrostimulators. Considerations on 4 cases]. The complications arising out of the installation of pacemakers are listed and classified and it is suggested that a syndrome characterized by recurrent pleural effusion recalcitrant to the usual medico-depletive therapies, observed in four patients with pacemakers, should be included among such complications. The clinical pictures of the syndrome is described in detail and particular attention is paid to its date of onset in relation to installation of the pacemaker, early symptomatology, the most typical symptoms, clinico-humoral development, prognosis and therapy. Some theoretical and practical remarks are also offered, specifically three pathogenetic suggestions are formulated on the basis of clinical and developmental features but mainly in relation to humoral and immunological findings. These suggestions stress alternative interpretations of the syndrome: A) as an autoimmune disease arising secondary to surgical denaturation of myocardial and/or endocardial autoantigens; B) as a systemic autoimmune disease due to congenital or acquired insufficiency of immunological homeostasis; C) as a monosymptomatic pleural form of systemic lupus erythematodes with late clinical outbreak. The fascinating pathogenetic, clinical, biological and therapeutic resemblances between the present syndrome and the post-infarctual syndrome of Dressler and Johnson's post-pericardiotomic syndrome are pointed out and it is suggested that complications of medical nature already described as being secondary to the installation of pacemakers, such as endocarditis and pericarditis, should be looked at from an autoimmune type of pathogenetic viewpoint. Finally, in the light of personal observations, the need for immunological study of high risk patients, namely relations of patients with autoimmune diseases, is stressed. All candidates for pacemaker installation should also be included. Routine screening of this type would take on enormous practical importance as it would enable preventive or curative treatment to be undertaken to modify the immunitary balance of those subjects in whom pacemaker installation is liable to trigger off critical, irreversible breakdown in immunitary homoeostasis of which the syndrome in question would seem to be the most dramatic, but perhaps no the only, clinical manifestation.", "PMID": 967339}
{"id": "pubmed23n0032_7009", "title": "[Recording of the potential of the bundle of His through the veins of the arm].", "content": "A new technique for recording His bundle and right branch potential through the veins of the arm instead of by the femoral route is described. Recordings were carried out in 70 patients. In 10, His potential was recorded simultaneously by the femoral and brachial routes. Potentials proved to be similar as regards morphology and duration; A-V conduction time proved identical while there were slight variations in His node conduction time since the atriogram revealed varying intrinsic deflexions. It is thus possible to confirm that the recording of His potential by the brachial route offers various advantages: 1) it takes less time than the femoral route; 2) it is an excellent alternative if, for various reasons, the patient cannot be examined by the femoral route; 3) His potential can be monitored more easily because of the greater stability of the catheter and the provisional electrosimulation using the same catheter when necessary; 4) contemporaneous recording of right branch and His bundle potential is possible in association with the femoral technique with better results than those obtained using multipolar catheters; 5) it is without complications: i.e. the phlebitis, phlebothrombosis, a-v fistula, and pulmonary emboly which, however rarely, may occur when using the femoral route.", "contents": "[Recording of the potential of the bundle of His through the veins of the arm]. A new technique for recording His bundle and right branch potential through the veins of the arm instead of by the femoral route is described. Recordings were carried out in 70 patients. In 10, His potential was recorded simultaneously by the femoral and brachial routes. Potentials proved to be similar as regards morphology and duration; A-V conduction time proved identical while there were slight variations in His node conduction time since the atriogram revealed varying intrinsic deflexions. It is thus possible to confirm that the recording of His potential by the brachial route offers various advantages: 1) it takes less time than the femoral route; 2) it is an excellent alternative if, for various reasons, the patient cannot be examined by the femoral route; 3) His potential can be monitored more easily because of the greater stability of the catheter and the provisional electrosimulation using the same catheter when necessary; 4) contemporaneous recording of right branch and His bundle potential is possible in association with the femoral technique with better results than those obtained using multipolar catheters; 5) it is without complications: i.e. the phlebitis, phlebothrombosis, a-v fistula, and pulmonary emboly which, however rarely, may occur when using the femoral route.", "PMID": 967340}
{"id": "pubmed23n0032_7010", "title": "[Supravalvular aortic stenosis. 6 operated cases].", "content": "6 cases of aortic supravalvular stenosis with associated abnormalities are reported. An aetiopathogenetic, anatomopathological and clinical discussion follows and the excellent results obtained with the therapy employed are reported.", "contents": "[Supravalvular aortic stenosis. 6 operated cases]. 6 cases of aortic supravalvular stenosis with associated abnormalities are reported. An aetiopathogenetic, anatomopathological and clinical discussion follows and the excellent results obtained with the therapy employed are reported.", "PMID": 967341}
{"id": "pubmed23n0032_7011", "title": "[Critical analysis of the diagnostic value of the flow-volume curve].", "content": "Changes in the flow-volume curve after administration of a bronchodilator were studied in reversible bronchial obstruction. The concept of isovolume is used in analysis of the curve indices, i.e. it is felt that the index (75, 60, 50, 25% FVC) after administration should be fixed on the curve at the same volume as on the basic curve to enable an indirect account to be taken of changes in flow in function of time, since, if resistance is reduced, the emitted volume varies in the same period even though the conditions promoting the flow are unchanged.", "contents": "[Critical analysis of the diagnostic value of the flow-volume curve]. Changes in the flow-volume curve after administration of a bronchodilator were studied in reversible bronchial obstruction. The concept of isovolume is used in analysis of the curve indices, i.e. it is felt that the index (75, 60, 50, 25% FVC) after administration should be fixed on the curve at the same volume as on the basic curve to enable an indirect account to be taken of changes in flow in function of time, since, if resistance is reduced, the emitted volume varies in the same period even though the conditions promoting the flow are unchanged.", "PMID": 967343}
{"id": "pubmed23n0032_7012", "title": "[Albers-Schoenberg disease in adults. Description of a case].", "content": "-- A benign form of Albers Schonberg disease (osteopetrosis) is described. The clinical and pathogenetical aspects are discussed and the findings compared with the literature. The diagnosis was made on the basis of radiological findings and histological results of bone biopsy.", "contents": "[Albers-Schoenberg disease in adults. Description of a case]. -- A benign form of Albers Schonberg disease (osteopetrosis) is described. The clinical and pathogenetical aspects are discussed and the findings compared with the literature. The diagnosis was made on the basis of radiological findings and histological results of bone biopsy.", "PMID": 967344}
{"id": "pubmed23n0032_7013", "title": "[Effect of S-adenosyl-methionine (SAMe) on some endocrine parameters in normal adult subjects].", "content": "No changes in serum prolactin, TSH and LH were noted when the antidepressant s-adenosylmethionine was given i.v. to 7 normal adults of both sexes. It would thus appear that the drug's antidepressant effect is not mediated by TSH. The results obtained are not comparable with those observed by other workers in the rat.", "contents": "[Effect of S-adenosyl-methionine (SAMe) on some endocrine parameters in normal adult subjects]. No changes in serum prolactin, TSH and LH were noted when the antidepressant s-adenosylmethionine was given i.v. to 7 normal adults of both sexes. It would thus appear that the drug's antidepressant effect is not mediated by TSH. The results obtained are not comparable with those observed by other workers in the rat.", "PMID": 967354}
{"id": "pubmed23n0032_7014", "title": "[Chronic alcoholic pancreatitis and blood gastrin].", "content": "On the basis of some experimental observations of hypergastrinemia in animals chronically intoxicated with ethanol, both fasting and after meals serum gastrin were determined in patients affected by chronic alcoholic pancreatitis. A significant increase in serum gastrin levels was observed in patients with chronic pancreatitis compared with controls, both in basal conditions and following food stimulation. The physiopathological hypotheses and possible aetiopathogenetic implications suggested by such gastrin behaviour are discussed.", "contents": "[Chronic alcoholic pancreatitis and blood gastrin]. On the basis of some experimental observations of hypergastrinemia in animals chronically intoxicated with ethanol, both fasting and after meals serum gastrin were determined in patients affected by chronic alcoholic pancreatitis. A significant increase in serum gastrin levels was observed in patients with chronic pancreatitis compared with controls, both in basal conditions and following food stimulation. The physiopathological hypotheses and possible aetiopathogenetic implications suggested by such gastrin behaviour are discussed.", "PMID": 967355}
{"id": "pubmed23n0032_7015", "title": "[Acute pancreatitis and hyperlipoproteinemia].", "content": "About 10 patients admitted with acute pancreatitis were also, found to have Fredrikson type V hyperlipoproteinaemia. Eight were males aged less than 45 yr. Awareness of the aetiopathogenetic and metabolic aspects of this increasingly common association and its clinical identification form the sine qua non in the prevention of recurrences via marked dietary restriction of fats.", "contents": "[Acute pancreatitis and hyperlipoproteinemia]. About 10 patients admitted with acute pancreatitis were also, found to have Fredrikson type V hyperlipoproteinaemia. Eight were males aged less than 45 yr. Awareness of the aetiopathogenetic and metabolic aspects of this increasingly common association and its clinical identification form the sine qua non in the prevention of recurrences via marked dietary restriction of fats.", "PMID": 967356}
{"id": "pubmed23n0032_7016", "title": "[Changes in vitro of the electrolyte content and erythrocyte volume of the venous blood of patients with chronic pneumopathy subjected to tonometry in oxygen].", "content": "As previously observed in vivo, statistically significant variations characterized by increase in chlorine and diminution in sodium, associated with an increase in red cell volume, have been noted in vitro in samples of venous blood taken from patients undergoing tonometry under oxygen. These variations appear to be independent of the basic concentration of chlorine and do not occur if the blood samples are incubated with theophylline at the same time. These results confirm the hypothesis of a \"non respiratory\" plasmatic acidifying effect of oxygen therapy inhibited by the antiphosphodiesterasic action of theophylline.", "contents": "[Changes in vitro of the electrolyte content and erythrocyte volume of the venous blood of patients with chronic pneumopathy subjected to tonometry in oxygen]. As previously observed in vivo, statistically significant variations characterized by increase in chlorine and diminution in sodium, associated with an increase in red cell volume, have been noted in vitro in samples of venous blood taken from patients undergoing tonometry under oxygen. These variations appear to be independent of the basic concentration of chlorine and do not occur if the blood samples are incubated with theophylline at the same time. These results confirm the hypothesis of a \"non respiratory\" plasmatic acidifying effect of oxygen therapy inhibited by the antiphosphodiesterasic action of theophylline.", "PMID": 967357}
{"id": "pubmed23n0032_7017", "title": "[Changes in paO2 and of acid-base equilibrium of the arterial blood of hypoxemic polyglobulic patients subjected to acute blood depletion].", "content": "Partial respiratory gas tension and acid-base equilibrium patterns were examined in polyglobulic patients subjected to acute blood depletion. The increase in oxyhaemoglobin concentration and fall in haematocrit value already described were confirmed. It was also noted that: 1) pH increased significantly in all subjects; 2) urinary osmolarity increased in all subjects; 3) blood sodium and potassium concentration increased significantly in 14 patients (1st group), but fell in the 2nd group (8 subjects). It is suggested, therefore, that acute blood depletion changes renal reabsorption of the main electrolytes, i.e. increased values in cases where a fall in glomerular arteriolar pressure leads to increased renal medullary osmolarity, and decreased values where a fall in circulation leads to increased arteriolar pressure, hyperosmolarity of the medulla, and retainment of liquids as opposed to electrolytes.", "contents": "[Changes in paO2 and of acid-base equilibrium of the arterial blood of hypoxemic polyglobulic patients subjected to acute blood depletion]. Partial respiratory gas tension and acid-base equilibrium patterns were examined in polyglobulic patients subjected to acute blood depletion. The increase in oxyhaemoglobin concentration and fall in haematocrit value already described were confirmed. It was also noted that: 1) pH increased significantly in all subjects; 2) urinary osmolarity increased in all subjects; 3) blood sodium and potassium concentration increased significantly in 14 patients (1st group), but fell in the 2nd group (8 subjects). It is suggested, therefore, that acute blood depletion changes renal reabsorption of the main electrolytes, i.e. increased values in cases where a fall in glomerular arteriolar pressure leads to increased renal medullary osmolarity, and decreased values where a fall in circulation leads to increased arteriolar pressure, hyperosmolarity of the medulla, and retainment of liquids as opposed to electrolytes.", "PMID": 967358}
{"id": "pubmed23n0032_7018", "title": "[Gilbert's jaundice. Current clinico-nosographical, physiopathological and therapeutic aspects. Note II. The phenomenon of enzyme induction at the level of the liver].", "content": "After a brief survey of the various modifications that may be encountered by drugs through the work of systems that detoxify the liver cell, attention is given to the problem of enzymic induction. The latter is the result of derepression of a gene that codes for a given enzyme; at the molecular level, derepression takes place when the substrate, by changing the tertiary structure of the repressor, brings about its detachment from DNA: in this way, RNA-polymerase is made capable of synthesising the corresponding mRNA. The inducing activity of phenobarbitone, a drug employed classically in the management of Gilbertian forms, must be substantially attributed to an increase in the synthesis of microsomial proteins, as shown by studies with labelled amino acids.", "contents": "[Gilbert's jaundice. Current clinico-nosographical, physiopathological and therapeutic aspects. Note II. The phenomenon of enzyme induction at the level of the liver]. After a brief survey of the various modifications that may be encountered by drugs through the work of systems that detoxify the liver cell, attention is given to the problem of enzymic induction. The latter is the result of derepression of a gene that codes for a given enzyme; at the molecular level, derepression takes place when the substrate, by changing the tertiary structure of the repressor, brings about its detachment from DNA: in this way, RNA-polymerase is made capable of synthesising the corresponding mRNA. The inducing activity of phenobarbitone, a drug employed classically in the management of Gilbertian forms, must be substantially attributed to an increase in the synthesis of microsomial proteins, as shown by studies with labelled amino acids.", "PMID": 967360}
{"id": "pubmed23n0032_7019", "title": "[Secretion of GH and diabetic retinopathy].", "content": "Recently it has been studied a possible pathogenetic role of GH in diabetic angiopathy. The purpose of the present study was to verify whether the diabetic retinopathy is associated or not with a high plasma GH level. In an attempt to answer this question we did the dosage of plasma GH after a oral glucose load (110 g), Insulin (0,1 U/Kg) i.v., and arginin i.v. (25 g/30'). The plasma GH measurements were approximately the same as in normal patients. The Authors underline the importance of GH in pathophysiology of diabetic angiopathy and report all recent literature on this argument.", "contents": "[Secretion of GH and diabetic retinopathy]. Recently it has been studied a possible pathogenetic role of GH in diabetic angiopathy. The purpose of the present study was to verify whether the diabetic retinopathy is associated or not with a high plasma GH level. In an attempt to answer this question we did the dosage of plasma GH after a oral glucose load (110 g), Insulin (0,1 U/Kg) i.v., and arginin i.v. (25 g/30'). The plasma GH measurements were approximately the same as in normal patients. The Authors underline the importance of GH in pathophysiology of diabetic angiopathy and report all recent literature on this argument.", "PMID": 967359}
{"id": "pubmed23n0032_7020", "title": "[Perforating plantar ulcers in alcoholic polyneuritis. Physiopathology; clinical and instrumental findings].", "content": "Notes on the anatomical and physiological features of the circulation are followed by a description of the main physiopathological aspects of the terminal circulation of the lower extremities. Reference is made to the pathogenesis and clinical and instrumental diagnosis of plantar ulcers in alcoholic polyneuritis, along with the symtoms constantly observed in a series of some 30 cases.", "contents": "[Perforating plantar ulcers in alcoholic polyneuritis. Physiopathology; clinical and instrumental findings]. Notes on the anatomical and physiological features of the circulation are followed by a description of the main physiopathological aspects of the terminal circulation of the lower extremities. Reference is made to the pathogenesis and clinical and instrumental diagnosis of plantar ulcers in alcoholic polyneuritis, along with the symtoms constantly observed in a series of some 30 cases.", "PMID": 967361}
{"id": "pubmed23n0032_7021", "title": "[Evaluation of the changes in some laboratory parameters in liver disease patients treated with Toxepasi Complex].", "content": "Toxepasi Complex (UDPG, glutathione and vitamin B12) was administered to patients with aggressive chronic hepatitis and a second group with severe cirrhosis. Changes in laboratory enzymological data indicative of the overall extent of liver damage due to cell necrosis, cholostasis and impairment of protein synthesis were evaluated. Decreases tending to normalisation were noted two weeks after the commencement of treatment, particularly in the first group. The results suggest that the complex may be usefully employed in the management of aggressive chronic hepatitis and cirrhosis of the liver.", "contents": "[Evaluation of the changes in some laboratory parameters in liver disease patients treated with Toxepasi Complex]. Toxepasi Complex (UDPG, glutathione and vitamin B12) was administered to patients with aggressive chronic hepatitis and a second group with severe cirrhosis. Changes in laboratory enzymological data indicative of the overall extent of liver damage due to cell necrosis, cholostasis and impairment of protein synthesis were evaluated. Decreases tending to normalisation were noted two weeks after the commencement of treatment, particularly in the first group. The results suggest that the complex may be usefully employed in the management of aggressive chronic hepatitis and cirrhosis of the liver.", "PMID": 967362}
{"id": "pubmed23n0032_7022", "title": "[Intestinal absorption of radiocalcium after long-term treatment with sodium fluoride in senile osteoporpsis].", "content": "Prolonged administration of sodium fluoride (NaF) in patients suffering from osteoporosis or Paget's disease leads to a positive calcium balance together with positivization of the calcium balance may mean an ins, intestinal absorption of calcium was evaluated directly with the oral rrotic patients; 6 months treatment with NaF led to a significant imprt is difficult to say how this finding is connected with the drug's actide apatite crystals; the crystals' greater stability probably leads to greater resistance of fluorated bone to the action of parathormone, thus bring on hypocalcaemia which homeostatically stimulates parathyroid hyperiirect pointer to parathyroid hyperincretion. The therapeutic effectiveness of NaF in diffuse decalcifying osteopathies is, however, limited by the drug's toxicity.", "contents": "[Intestinal absorption of radiocalcium after long-term treatment with sodium fluoride in senile osteoporpsis]. Prolonged administration of sodium fluoride (NaF) in patients suffering from osteoporosis or Paget's disease leads to a positive calcium balance together with positivization of the calcium balance may mean an ins, intestinal absorption of calcium was evaluated directly with the oral rrotic patients; 6 months treatment with NaF led to a significant imprt is difficult to say how this finding is connected with the drug's actide apatite crystals; the crystals' greater stability probably leads to greater resistance of fluorated bone to the action of parathormone, thus bring on hypocalcaemia which homeostatically stimulates parathyroid hyperiirect pointer to parathyroid hyperincretion. The therapeutic effectiveness of NaF in diffuse decalcifying osteopathies is, however, limited by the drug's toxicity.", "PMID": 967363}
{"id": "pubmed23n0032_7023", "title": "[Trial of a combination of proscillaridin A, thioridazine and Cori ester in the ambulatory treatment of some cardiopathies, especially senile types].", "content": "The effect of an association of proscillaridin A, thioridazine and Cori ester in ambulatory management of certain heart diseases, particularly in aged subjects, was assessed. Satisfactory results were observed in 58.3% of cases, with significant improvement of cardiac erethism, psychomotor instability and sleep disturbances, as well as cardiac frequency and signs of myocardial insufficiency. It is felt that the association is of assistance in the treatment of certain heart diseases unaccompanied by frank signs of insufficiency, especially in aged subjects.", "contents": "[Trial of a combination of proscillaridin A, thioridazine and Cori ester in the ambulatory treatment of some cardiopathies, especially senile types]. The effect of an association of proscillaridin A, thioridazine and Cori ester in ambulatory management of certain heart diseases, particularly in aged subjects, was assessed. Satisfactory results were observed in 58.3% of cases, with significant improvement of cardiac erethism, psychomotor instability and sleep disturbances, as well as cardiac frequency and signs of myocardial insufficiency. It is felt that the association is of assistance in the treatment of certain heart diseases unaccompanied by frank signs of insufficiency, especially in aged subjects.", "PMID": 967364}
{"id": "pubmed23n0032_7024", "title": "[Preliminary comparative results of the detection of hepatitis B antigen (HBAg) in the blood of mental patients, using several methods in common use].", "content": "Radioimmunoassay (RIA), immunoelectrophoresis (IEP) and latex agglutination (L-HHA) were compared in the detection of hepatitis B antigen in sera from 494 mental patients. Positivity was observed in 39 subjects (7.9%) with RIA, 14 (2.8%) with IEP, and 8 (1.6%) with L-HHA. The dilution titres noted with RIA were many times and significantly higher than those obtained with the other two methods. Antigen was again detected in over 50% of those subjects on whom it was possible to repeat the tests one year later. The advantages of RIA are stressed.", "contents": "[Preliminary comparative results of the detection of hepatitis B antigen (HBAg) in the blood of mental patients, using several methods in common use]. Radioimmunoassay (RIA), immunoelectrophoresis (IEP) and latex agglutination (L-HHA) were compared in the detection of hepatitis B antigen in sera from 494 mental patients. Positivity was observed in 39 subjects (7.9%) with RIA, 14 (2.8%) with IEP, and 8 (1.6%) with L-HHA. The dilution titres noted with RIA were many times and significantly higher than those obtained with the other two methods. Antigen was again detected in over 50% of those subjects on whom it was possible to repeat the tests one year later. The advantages of RIA are stressed.", "PMID": 967365}
{"id": "pubmed23n0032_7025", "title": "[GH secretion after L-dopa administration in hepatic cirrhosis].", "content": "A 500 mg L-Dopa administration in normal and cirrhotic subjects does not determinate a significant varation of plasma glucose and insulin level, while a peak plasma GH level in both cirrhotic and normal subjects occurred at 90' with a significantly greates values in cirrhotics. It is doubtful to affirm that a high plasma GH level is the only one factor which responsable of glucose intolerance in cirrhotic subjects, when there are many others factors contribute to it in synergic way. In order to elucidate the hypothesis on possible pathogenetic mechanism it is discussed some of our own experience and observations.", "contents": "[GH secretion after L-dopa administration in hepatic cirrhosis]. A 500 mg L-Dopa administration in normal and cirrhotic subjects does not determinate a significant varation of plasma glucose and insulin level, while a peak plasma GH level in both cirrhotic and normal subjects occurred at 90' with a significantly greates values in cirrhotics. It is doubtful to affirm that a high plasma GH level is the only one factor which responsable of glucose intolerance in cirrhotic subjects, when there are many others factors contribute to it in synergic way. In order to elucidate the hypothesis on possible pathogenetic mechanism it is discussed some of our own experience and observations.", "PMID": 967366}
{"id": "pubmed23n0032_7026", "title": "[Hepatic distomiasis caused by Fasciola hepatica].", "content": "A case of bile duct obstruction due to Fasciola hepatica is presented. The evolution, aetiopathogenesis, symptomatology, diagnosis and therapy of this rare syndrome are then discribed.", "contents": "[Hepatic distomiasis caused by Fasciola hepatica]. A case of bile duct obstruction due to Fasciola hepatica is presented. The evolution, aetiopathogenesis, symptomatology, diagnosis and therapy of this rare syndrome are then discribed.", "PMID": 967367}
{"id": "pubmed23n0032_7027", "title": "Plasma cortisol levels in human fetus during parturition.", "content": "In several mammalian species, the fetal adrenal cortex plays an important role in the spontaneous onset of labor. No conclusive evidence has yet been presented that a similar mechanism is active in humans. Although in some previous studies the cortisol concentration in human cord blood has been found higher after spontaneous labor than after induced labor, it has not been possible to determine whether this rise in the fetal cortisol level precedes the onset of parturition or is a consequence of labor. In the present study, starting at the earliest possible stage of labor, fetal scalp blood samples were taken sequentially during 16 spontaneous and 13 induced vaginal deliveries. Cortisol levels in these 2 goups of samples were compared with each other and also with cord blood cortisol levels in 11 patients undergoing elective cesarean section. The initial fetal cortisol levels did not differ between groups with different modes of labor onset. A pronounced rise of plasma cortisol levels occurred during labor in simultaneously sampled maternal and fetal blood. These results do not support the concept of a role for the fetal adrenal cortex in the initiation of labor in humans; they invalidate the use of cortisol concentration in cord blood for the estimation of prelabor fetal cortisol level. The origin of the cortisol surge in fetal plasma during labor and also whether the fetal adrenocortical function responds to the stress of labor are discussed. It is concluded that the rise of fetal cortisol levels during labor might mainly be a reflection of the maternal response to stress.", "contents": "Plasma cortisol levels in human fetus during parturition. In several mammalian species, the fetal adrenal cortex plays an important role in the spontaneous onset of labor. No conclusive evidence has yet been presented that a similar mechanism is active in humans. Although in some previous studies the cortisol concentration in human cord blood has been found higher after spontaneous labor than after induced labor, it has not been possible to determine whether this rise in the fetal cortisol level precedes the onset of parturition or is a consequence of labor. In the present study, starting at the earliest possible stage of labor, fetal scalp blood samples were taken sequentially during 16 spontaneous and 13 induced vaginal deliveries. Cortisol levels in these 2 goups of samples were compared with each other and also with cord blood cortisol levels in 11 patients undergoing elective cesarean section. The initial fetal cortisol levels did not differ between groups with different modes of labor onset. A pronounced rise of plasma cortisol levels occurred during labor in simultaneously sampled maternal and fetal blood. These results do not support the concept of a role for the fetal adrenal cortex in the initiation of labor in humans; they invalidate the use of cortisol concentration in cord blood for the estimation of prelabor fetal cortisol level. The origin of the cortisol surge in fetal plasma during labor and also whether the fetal adrenocortical function responds to the stress of labor are discussed. It is concluded that the rise of fetal cortisol levels during labor might mainly be a reflection of the maternal response to stress.", "PMID": 967373}
{"id": "pubmed23n0032_7028", "title": "Serum cortisol and 11-desoxycortisol levels in hirsute premenopausal women.", "content": "It has been postulated that hirsute patients may have a relative deficiency in 11beta-hydroxylase activity of the adrenal cortex. In order to test this postulate, we have measured the serum levels of cortisol (Cp F) and 11-desoxycortisol (Cp S) and estimated the Cp S/Cp F ratio in 9 nonhirsute and 34 hirsute premenopausal women. As a group, the hirsute patients had significantly elevated (P less than 0.05) mean Cp F and Cp S levels but the mean Cp S/Cp F ratio was not significantly different from normal. Considered individually, only 3 hirsute patients had a Cp S/Cp F ratio greater than 2 SD above the mean normal levels. These ratios were 0.0218, 0.0139, and 0.023. If there is indeed an 11beta-hydroxylase deficiency in these 3 patients, it must be relatively minor, since a patient with documented 11beta-hydroxylase deficiency had Cp S levels of 218 ng/ml and a Cp S/Cp F ratio of 0.7. Our data suggest that 11beta-hydroxylase deficiency is not a common cause of hirsutism.", "contents": "Serum cortisol and 11-desoxycortisol levels in hirsute premenopausal women. It has been postulated that hirsute patients may have a relative deficiency in 11beta-hydroxylase activity of the adrenal cortex. In order to test this postulate, we have measured the serum levels of cortisol (Cp F) and 11-desoxycortisol (Cp S) and estimated the Cp S/Cp F ratio in 9 nonhirsute and 34 hirsute premenopausal women. As a group, the hirsute patients had significantly elevated (P less than 0.05) mean Cp F and Cp S levels but the mean Cp S/Cp F ratio was not significantly different from normal. Considered individually, only 3 hirsute patients had a Cp S/Cp F ratio greater than 2 SD above the mean normal levels. These ratios were 0.0218, 0.0139, and 0.023. If there is indeed an 11beta-hydroxylase deficiency in these 3 patients, it must be relatively minor, since a patient with documented 11beta-hydroxylase deficiency had Cp S levels of 218 ng/ml and a Cp S/Cp F ratio of 0.7. Our data suggest that 11beta-hydroxylase deficiency is not a common cause of hirsutism.", "PMID": 967374}
{"id": "pubmed23n0032_7029", "title": "Incidence of endometriosis among Japanese women.", "content": "Clinical experience based on gynecologic practice in Hawaii indicated a high incidence of endometriosis among Oriental women. To investigate this impression, a statistical analysis of gynecologic admissions and of diagnoses of endometriosis was performed on the basis of race at three hospitals, two in Hawaii and one in Japan. The result supports the impression that the Japanese female population has a high incidence of endometriosis. Approximately 10% of their gynecologic admissions were for endometriosis. The order of incidence in other racial groups was non-Japanese Oriental, white, and black.", "contents": "Incidence of endometriosis among Japanese women. Clinical experience based on gynecologic practice in Hawaii indicated a high incidence of endometriosis among Oriental women. To investigate this impression, a statistical analysis of gynecologic admissions and of diagnoses of endometriosis was performed on the basis of race at three hospitals, two in Hawaii and one in Japan. The result supports the impression that the Japanese female population has a high incidence of endometriosis. Approximately 10% of their gynecologic admissions were for endometriosis. The order of incidence in other racial groups was non-Japanese Oriental, white, and black.", "PMID": 967375}
{"id": "pubmed23n0032_7030", "title": "Venereal transmission of hepatitis B virus. The possible role of vaginal secretions.", "content": "The presence of Type B viral (Australia antigen related serum) hepatitis in vaginal specimens was investigated and compared with that in saliva specimens in a group of patients during the acute phase of their illness. Hepatitis B surface antigen was detectable in most vaginal and salivary secretions during the phase of antigenemia and not in hepatitis B surface antigen-negative controls. Despite care in collection, occult blood was detected in the majority of specimens, but the positivity for hepatitis B surface antigen did not depend on the presence of occult blood. Recovery studies were performed in both vaginal fluids and saliva to determine if inhibitors to hepatitis B surface antigen were similar to those observed in stool samples. Significant inhibition was not observed. Although infectivity of the specimens was not established from this study, handling of these specimens as potentially infective material is recommended.", "contents": "Venereal transmission of hepatitis B virus. The possible role of vaginal secretions. The presence of Type B viral (Australia antigen related serum) hepatitis in vaginal specimens was investigated and compared with that in saliva specimens in a group of patients during the acute phase of their illness. Hepatitis B surface antigen was detectable in most vaginal and salivary secretions during the phase of antigenemia and not in hepatitis B surface antigen-negative controls. Despite care in collection, occult blood was detected in the majority of specimens, but the positivity for hepatitis B surface antigen did not depend on the presence of occult blood. Recovery studies were performed in both vaginal fluids and saliva to determine if inhibitors to hepatitis B surface antigen were similar to those observed in stool samples. Significant inhibition was not observed. Although infectivity of the specimens was not established from this study, handling of these specimens as potentially infective material is recommended.", "PMID": 967376}
{"id": "pubmed23n0032_7031", "title": "Intrauterine adhesions secondary to elective abortion. Hysteroscopic diagnosis and management.", "content": "Ten patients who developed Asherman's syndrome following elective first trimester abortion underwent outpatient hysteroscopy under local anesthesia. Six of the 10 patients had hysterosalpingograms prior to surgery. Correlation between the radiographic findings and those at hysteroscopy was poor. Treatment consisted of hysteroscopic lysis of adhesions, placement of an IUD or Foley catheter, and sequential estrogen-progestin administration. Normal menses were resumed in all women. Hysteroscopy was also used for followup in 4 patients. Outpatient hysteroscopy under local anesthesia is safe and is the method of choice for diagnosing, treating, and following patients with Asherman's syndrome.", "contents": "Intrauterine adhesions secondary to elective abortion. Hysteroscopic diagnosis and management. Ten patients who developed Asherman's syndrome following elective first trimester abortion underwent outpatient hysteroscopy under local anesthesia. Six of the 10 patients had hysterosalpingograms prior to surgery. Correlation between the radiographic findings and those at hysteroscopy was poor. Treatment consisted of hysteroscopic lysis of adhesions, placement of an IUD or Foley catheter, and sequential estrogen-progestin administration. Normal menses were resumed in all women. Hysteroscopy was also used for followup in 4 patients. Outpatient hysteroscopy under local anesthesia is safe and is the method of choice for diagnosing, treating, and following patients with Asherman's syndrome.", "PMID": 967377}
{"id": "pubmed23n0032_7032", "title": "Pregnancy following jejunoileal bypass. Effects on fetal outcome.", "content": "Four cases of pregnancy following jejunoileal bypass surgery for obesity are presented, and 9 cases from the recent literature are reviewed. Pregnancy after bypass surgery often resulted in low-birthweight infants (46%) at term, and this was especially true when conception occurred within 2 years of the surgery. In light of these findings it may be advisable to postpone pregnancy until after the second postoperative year.", "contents": "Pregnancy following jejunoileal bypass. Effects on fetal outcome. Four cases of pregnancy following jejunoileal bypass surgery for obesity are presented, and 9 cases from the recent literature are reviewed. Pregnancy after bypass surgery often resulted in low-birthweight infants (46%) at term, and this was especially true when conception occurred within 2 years of the surgery. In light of these findings it may be advisable to postpone pregnancy until after the second postoperative year.", "PMID": 967378}
{"id": "pubmed23n0032_7033", "title": "Progesterone levels in amniotic fluid and maternal plasma in prostaglandin F2alpha-induced midtrimester abortion.", "content": "Progesterone concentrations in amniotic fluid and maternal plasma were determined in 11 midtrimester pregnant patients following the intraamniotic administration of prostaglandin F2alpha. Samples were obtained at 3-hour intervals until abortion or spontaneous rupture of membranes occurred or fetal heart tones disappeared. In amniotic fluid, the mean progesterone concentrations increased throughout the sampling period. The plasma progesterone levels declined by about one-third of basal values in the first 3 hours after prostaglandin administration. The paradoxic increase in amniotic fluid progesterone is probably secondary to alterations in uterine blood flow and intrauterine pressure.", "contents": "Progesterone levels in amniotic fluid and maternal plasma in prostaglandin F2alpha-induced midtrimester abortion. Progesterone concentrations in amniotic fluid and maternal plasma were determined in 11 midtrimester pregnant patients following the intraamniotic administration of prostaglandin F2alpha. Samples were obtained at 3-hour intervals until abortion or spontaneous rupture of membranes occurred or fetal heart tones disappeared. In amniotic fluid, the mean progesterone concentrations increased throughout the sampling period. The plasma progesterone levels declined by about one-third of basal values in the first 3 hours after prostaglandin administration. The paradoxic increase in amniotic fluid progesterone is probably secondary to alterations in uterine blood flow and intrauterine pressure.", "PMID": 967379}
{"id": "pubmed23n0032_7034", "title": "Gonococcemia in pregnancy.", "content": "A case of gonococcemia in pregnancy in which the patient presented with the classic clinical manifestations of gonococcemia--cutaneous lesions, arthritis, and possibly pericarditis--is reported. A review of the literature reveals a dramatic increase in incidence of gonorrhea and the increasing importance of the complications associated with this disease, particularly in pregnancy. Recommendations regarding recognition of gonococcemia, criteria for diagnosis, and treatment are presented.", "contents": "Gonococcemia in pregnancy. A case of gonococcemia in pregnancy in which the patient presented with the classic clinical manifestations of gonococcemia--cutaneous lesions, arthritis, and possibly pericarditis--is reported. A review of the literature reveals a dramatic increase in incidence of gonorrhea and the increasing importance of the complications associated with this disease, particularly in pregnancy. Recommendations regarding recognition of gonococcemia, criteria for diagnosis, and treatment are presented.", "PMID": 967380}
{"id": "pubmed23n0032_7035", "title": "The effect of drugs on uterine activity.", "content": "Uterine activity was measured for a 30-minute preinjection period and a 30-minute postinjection period for six drugs commonly used in labor. Uterine activity was quantitated by measuring the area below the uterine pressure curve with an on-line technic utilizing a voltage control oscillator. Uterine activity readings were obtained every 2.5 minutes and reported in uterine activity units (UAU). Unmedicated labor demonstrated a relatively static increase in UAU per unit of time in labor. On comparing total UAU before and after injection, only magnesium sulfate was noted to cause a decrease in total UAU. Utilizing 10-minute summations of UAU, the pre- and postinjection periods were compared to the calculated expected UAU for the 30 minutes following the preinjection period (assuming no drug had been used). For each of the other drugs, the positive slope of the calculated expected UAU shifted to a negative slope of the actual observed UAU following drug administration.", "contents": "The effect of drugs on uterine activity. Uterine activity was measured for a 30-minute preinjection period and a 30-minute postinjection period for six drugs commonly used in labor. Uterine activity was quantitated by measuring the area below the uterine pressure curve with an on-line technic utilizing a voltage control oscillator. Uterine activity readings were obtained every 2.5 minutes and reported in uterine activity units (UAU). Unmedicated labor demonstrated a relatively static increase in UAU per unit of time in labor. On comparing total UAU before and after injection, only magnesium sulfate was noted to cause a decrease in total UAU. Utilizing 10-minute summations of UAU, the pre- and postinjection periods were compared to the calculated expected UAU for the 30 minutes following the preinjection period (assuming no drug had been used). For each of the other drugs, the positive slope of the calculated expected UAU shifted to a negative slope of the actual observed UAU following drug administration.", "PMID": 967381}
{"id": "pubmed23n0032_7036", "title": "Oxytocin-induced uterine hypercontractility pressure wave forms.", "content": "To identify similarities and differences between uterine contractions in patients with spontaneous labor, oxytocin-corrected hypocontractility, and oxytocin-induced hypercontractility, measurements were made of specific characteristics of the intrauterine wave form, including pressure, rate of change of pressure, and duration. Contractions from oxytocin-treated patients differed from contractions of those with spontaneous labor in their disproportionately high rates of rise of the pressure. The time to reach the maximum rate of the pressure--the start-up time--was reduced in oxytocin-induced hypercontractility. The data demonstrate that the contractions of spontaneous labor and oxytocin-augmented labor are not identical, and the physiologic implications are discussed.", "contents": "Oxytocin-induced uterine hypercontractility pressure wave forms. To identify similarities and differences between uterine contractions in patients with spontaneous labor, oxytocin-corrected hypocontractility, and oxytocin-induced hypercontractility, measurements were made of specific characteristics of the intrauterine wave form, including pressure, rate of change of pressure, and duration. Contractions from oxytocin-treated patients differed from contractions of those with spontaneous labor in their disproportionately high rates of rise of the pressure. The time to reach the maximum rate of the pressure--the start-up time--was reduced in oxytocin-induced hypercontractility. The data demonstrate that the contractions of spontaneous labor and oxytocin-augmented labor are not identical, and the physiologic implications are discussed.", "PMID": 967382}
{"id": "pubmed23n0032_7037", "title": "\"Prediction\" of the one-minute Apgar score from fetal heart rate data.", "content": "The value of any fetal monitoring technic is in its ability to predict infant outcome. In the present study, the ability of fetal heart rate (FHR) monitoring data to \"predict\" a measure of short-term infant outcome, the 1-minute Apgar score, was evaluated using univariate and multivariate statistical analyses. Of 61 monitored high-risk infants, 46 had high (7 to 10) and 15 had low (1 to 6) 1-minute Apgar scores. Computer analysis of FHR/intrauterine pressure (IUP) data for these 61 infants revealed that the infants with low Apgar scores had more than the expected number of late decelerations (LD). Using a threshold of ten LD and univariate analysis, 74% of the infants could be properly classified for high or low Apgar scores, but 60% of the infants with low Apgar scores were not identified. Using discriminant function (multivariate) analysis for the numbers of LD and uterine contractions, 47% of the depressed infants were appropriately identified and simple risk scoring equations were devised. Using additional observation vectors, including the number of accelerations and early decelerations, 67% of the depressed infants could be identified. The results of this study suggest that using multiple observation vectors improves the predictive capacity and, thus, the value of fetal monitoring data. Clinical experience suggests that the value of monitoring data can be further enhanced by simultaneous evaluation of other observation vectors from additional perinatal data sets using the technics of this study.", "contents": "\"Prediction\" of the one-minute Apgar score from fetal heart rate data. The value of any fetal monitoring technic is in its ability to predict infant outcome. In the present study, the ability of fetal heart rate (FHR) monitoring data to \"predict\" a measure of short-term infant outcome, the 1-minute Apgar score, was evaluated using univariate and multivariate statistical analyses. Of 61 monitored high-risk infants, 46 had high (7 to 10) and 15 had low (1 to 6) 1-minute Apgar scores. Computer analysis of FHR/intrauterine pressure (IUP) data for these 61 infants revealed that the infants with low Apgar scores had more than the expected number of late decelerations (LD). Using a threshold of ten LD and univariate analysis, 74% of the infants could be properly classified for high or low Apgar scores, but 60% of the infants with low Apgar scores were not identified. Using discriminant function (multivariate) analysis for the numbers of LD and uterine contractions, 47% of the depressed infants were appropriately identified and simple risk scoring equations were devised. Using additional observation vectors, including the number of accelerations and early decelerations, 67% of the depressed infants could be identified. The results of this study suggest that using multiple observation vectors improves the predictive capacity and, thus, the value of fetal monitoring data. Clinical experience suggests that the value of monitoring data can be further enhanced by simultaneous evaluation of other observation vectors from additional perinatal data sets using the technics of this study.", "PMID": 967383}
{"id": "pubmed23n0032_7038", "title": "Fetal heart rate response to amniocentesis in cases of decreased fetal movements.", "content": "Eleven women with fetal distress as manifested by a decrease of fetal movements up to cessation underwent amniocentesis for amniotic fluid evaluation under continuous FHR monitoring. All the fetuses showed abnormal heart rate response, manifested by absence of FHR acceleration or early or late deceleration. The pathologic response, or lack of response of fetal heart rate during amniocentesis is suggested as an additional test to evaluate the severity of fetal distress.", "contents": "Fetal heart rate response to amniocentesis in cases of decreased fetal movements. Eleven women with fetal distress as manifested by a decrease of fetal movements up to cessation underwent amniocentesis for amniotic fluid evaluation under continuous FHR monitoring. All the fetuses showed abnormal heart rate response, manifested by absence of FHR acceleration or early or late deceleration. The pathologic response, or lack of response of fetal heart rate during amniocentesis is suggested as an additional test to evaluate the severity of fetal distress.", "PMID": 967384}
{"id": "pubmed23n0032_7039", "title": "The effect of diazoxide on uterine blood flow in pregnant sheep.", "content": "Diazoxide, a labor inhibiting agent, was administered intravenously at various rates to seven pregnant, near-term sheep to evaluate its effect on cardiovascular and uterine hemodynamics. Uterine blood flow was measured with electromagnetic flow transducers. Rapid administration of diazoxide resulted in a profound maternal tachycardia with hypotension, an increase in uterine vascular resistance, and a significant decrease in uterine blood flow. With slow infusion of the drug, the changes in heart rate and blood pressure were minimized, uterine vascular resistance was decreased, and uterine blood flow was maintained. Therefore, slow infusion appears to be the preferred method for inhibiting labor with diazoxide.", "contents": "The effect of diazoxide on uterine blood flow in pregnant sheep. Diazoxide, a labor inhibiting agent, was administered intravenously at various rates to seven pregnant, near-term sheep to evaluate its effect on cardiovascular and uterine hemodynamics. Uterine blood flow was measured with electromagnetic flow transducers. Rapid administration of diazoxide resulted in a profound maternal tachycardia with hypotension, an increase in uterine vascular resistance, and a significant decrease in uterine blood flow. With slow infusion of the drug, the changes in heart rate and blood pressure were minimized, uterine vascular resistance was decreased, and uterine blood flow was maintained. Therefore, slow infusion appears to be the preferred method for inhibiting labor with diazoxide.", "PMID": 967385}
{"id": "pubmed23n0032_7040", "title": "The synergistic effect of calcium and prostaglandin F2alpha in second trimester abortion. A pilot study.", "content": "A clinical use of the basic science phenomenon that prostaglandin is synergistic with calcium in increasing uterine contractility has been demonstrated. Twenty-five women had midtrimester abortions induced with intraamniotic calcium gluconate followed by prostaglandin F2alpha. All patients aborted within 21 hours with minimal toxicity. The mean injection-to-abortion interval was 13.5 hours in the group of women without a preinjection laminaria and 10.1 hours in patients when a laminaria was included in the protocol.", "contents": "The synergistic effect of calcium and prostaglandin F2alpha in second trimester abortion. A pilot study. A clinical use of the basic science phenomenon that prostaglandin is synergistic with calcium in increasing uterine contractility has been demonstrated. Twenty-five women had midtrimester abortions induced with intraamniotic calcium gluconate followed by prostaglandin F2alpha. All patients aborted within 21 hours with minimal toxicity. The mean injection-to-abortion interval was 13.5 hours in the group of women without a preinjection laminaria and 10.1 hours in patients when a laminaria was included in the protocol.", "PMID": 967386}
{"id": "pubmed23n0032_7041", "title": "Prostaglandin E2 induction of labor for fetal demise.", "content": "Intrauterine fetal demise is a source of anxiety to both patient and physician. Heretofore, the standard treatment was either careful observation until the patient went into labor or attempt at induction of labor with oxytocin. Unfortunately, oxytocin stimulation has not proven to be uniformly successful for this problem. Prostaglandin E2 suppositories have been shown to be effective in inducing uterine evacuation after intrauterine fetal demise. In the opinion of the authors, this approach will in the future replace the sometimes dangerous and emotionally laden convention of watchful delayed therapy.", "contents": "Prostaglandin E2 induction of labor for fetal demise. Intrauterine fetal demise is a source of anxiety to both patient and physician. Heretofore, the standard treatment was either careful observation until the patient went into labor or attempt at induction of labor with oxytocin. Unfortunately, oxytocin stimulation has not proven to be uniformly successful for this problem. Prostaglandin E2 suppositories have been shown to be effective in inducing uterine evacuation after intrauterine fetal demise. In the opinion of the authors, this approach will in the future replace the sometimes dangerous and emotionally laden convention of watchful delayed therapy.", "PMID": 967387}
{"id": "pubmed23n0032_7042", "title": "Gonadal calcification. Indication for gonadectomy in gonadal dysgenesis.", "content": "Germ cell tumors, including gonadoblastoma, are usually found in patients with gonadal dysgenesis who have Y chromatin in their genotype. Diffuse calcification is a common finding in gonadoblastomas. Bilateral calcifications in the gonadal sites were seen on the intravenous pyelogram of a patient with 46,XX pure gonadal dysgenesis. Although the calcification was present in the fimbria of the fallopian tube, we suggest that gonadectomy is indicated in the patient with gonadal dysgenesis and probably gonadal calcification.", "contents": "Gonadal calcification. Indication for gonadectomy in gonadal dysgenesis. Germ cell tumors, including gonadoblastoma, are usually found in patients with gonadal dysgenesis who have Y chromatin in their genotype. Diffuse calcification is a common finding in gonadoblastomas. Bilateral calcifications in the gonadal sites were seen on the intravenous pyelogram of a patient with 46,XX pure gonadal dysgenesis. Although the calcification was present in the fimbria of the fallopian tube, we suggest that gonadectomy is indicated in the patient with gonadal dysgenesis and probably gonadal calcification.", "PMID": 967390}
{"id": "pubmed23n0032_7043", "title": "Scanning electron microscopy of melanoblastomas of the choroid.", "content": "With the scanning electron microscopic study of a case of the fascicular and two cases of epithelioid melanoblastomas it has been found that the shape of the cells is more variable than it was believed before on the basis of light microscopy. Certain types of arrangement described in histological observations, e.g. the fascicular type, can also be well demonstrated by scanning electron microscopy.", "contents": "Scanning electron microscopy of melanoblastomas of the choroid. With the scanning electron microscopic study of a case of the fascicular and two cases of epithelioid melanoblastomas it has been found that the shape of the cells is more variable than it was believed before on the basis of light microscopy. Certain types of arrangement described in histological observations, e.g. the fascicular type, can also be well demonstrated by scanning electron microscopy.", "PMID": 967399}
{"id": "pubmed23n0032_7044", "title": "Peripheral vision in children.", "content": "A comparison of peripheral vision in adults and children showed that children had reduced sensitivity in a standard visual field examination. The constriction of the visual field amounted to 0.4 log units at 30 degrees excentricity in a static perimetric test. This loss was maintained in a spatial forced choice experiment without foreknowledge of target location. However, in a temporal forced choice experiment with foreknowledge of target position the difference between children and adults was reduced to 0.1 log units. It is thought, therefore, that the former visual loss in children is due to the nature of the task rather than to deficiencies in the visual mechanisms.", "contents": "Peripheral vision in children. A comparison of peripheral vision in adults and children showed that children had reduced sensitivity in a standard visual field examination. The constriction of the visual field amounted to 0.4 log units at 30 degrees excentricity in a static perimetric test. This loss was maintained in a spatial forced choice experiment without foreknowledge of target location. However, in a temporal forced choice experiment with foreknowledge of target position the difference between children and adults was reduced to 0.1 log units. It is thought, therefore, that the former visual loss in children is due to the nature of the task rather than to deficiencies in the visual mechanisms.", "PMID": 967400}
{"id": "pubmed23n0032_7045", "title": "Are the oldest 'fossils', fossils?", "content": "A comparative statistical study has been carried out on populations of modern algae, of Precambrian algal microfossils, of the 'organized elements' of the Orgueil carbonaceous meteorite, and of the oldest microfossil-like objects now known (spheroidal bodies from the Fig Tree and Onverwacht Groups of the Swaziland Supergroup, South Africa). The distribution patterns exhibited by the greater than 3000 m.y.-old Swaziland microstructures bear considerable resemblance to those of the abiotic 'organized elements' but rather markedly differ from those exhibited by younger, assuredly biogenic, populations. Based on these comparisons it is concluded that the Swaziland spheroids could be, at least in part, of non-biologic origin; these oldest known fossil-like micro-structures should not be regarded as constituting firm evidence of Archean life.", "contents": "Are the oldest 'fossils', fossils? A comparative statistical study has been carried out on populations of modern algae, of Precambrian algal microfossils, of the 'organized elements' of the Orgueil carbonaceous meteorite, and of the oldest microfossil-like objects now known (spheroidal bodies from the Fig Tree and Onverwacht Groups of the Swaziland Supergroup, South Africa). The distribution patterns exhibited by the greater than 3000 m.y.-old Swaziland microstructures bear considerable resemblance to those of the abiotic 'organized elements' but rather markedly differ from those exhibited by younger, assuredly biogenic, populations. Based on these comparisons it is concluded that the Swaziland spheroids could be, at least in part, of non-biologic origin; these oldest known fossil-like micro-structures should not be regarded as constituting firm evidence of Archean life.", "PMID": 967401}
{"id": "pubmed23n0032_7046", "title": "Abiotic origin of biopolymers.", "content": "A variety of methods have been investigated in different laboratories for the polymerization of amino acids and nucleotides under abiotic conditions. They include (1) thermal polymerization, (2) direct polymerization of certain amino acid nitriles, amides or esters, (3) pi, Tokyo 194, Japan.", "contents": "Abiotic origin of biopolymers. A variety of methods have been investigated in different laboratories for the polymerization of amino acids and nucleotides under abiotic conditions. They include (1) thermal polymerization, (2) direct polymerization of certain amino acid nitriles, amides or esters, (3) pi, Tokyo 194, Japan.", "PMID": 967403}
{"id": "pubmed23n0032_7047", "title": "Evolutionary oscillation in prebiology: igneous activity and the origins of life.", "content": "The processes of chemical evolution are responsible for the origin of life. Three such processes have special importance: oscillation, creation, and competition. An oscillation from one kind of environment to another provides a mechanism for instituting processes that can only take place under conditions far removed from equilibrium. Oscillating evolutionary processes are likely to have played an important part in the origin of life. It is a mistake to assume that life originated in any one environment. It did not arrive in a moment of time. It was the result of a long period of chemical evolution during which it passed through a variety of environments. Biopoesis took place in an environment in which a variety of different kinds of protolife were assembled and concentrated. One essential form of protolife involved in these processes is the protocell. The experiments of Fox suggest that the creation of protocells involves violent oscillations of temperature and hydration. Igneous activity is especially characterised by oscillating conditions. Volcanic eruptions consist of violent changes from one extreme condition to another. Temperatures, pressure, phase, concentration and hydration all oscillate violently, and are subject to shock pulses of many kinds. Protolife may well have passed through extremes of environment for wider that those that life itself can sustain. The most probable environment for the assembly of the various forms of protolife would be on mudbanks forming either at the mouth of streams draining regions of active vulcanicity, or round the edge of hot volclanic pools. In this situation one could fins concentrated not only the various stands of protolife necessary for the final act of biopoesis, but also perbiologically formed nutrients necessary as for the first eobionts. As soon as the first protocells start to grow, they start to compete with each other, and so initiate a new additional evolutionary process, that of natural selection. Only after such competition has been initiated is life itself likely to be established\"20", "contents": "Evolutionary oscillation in prebiology: igneous activity and the origins of life. The processes of chemical evolution are responsible for the origin of life. Three such processes have special importance: oscillation, creation, and competition. An oscillation from one kind of environment to another provides a mechanism for instituting processes that can only take place under conditions far removed from equilibrium. Oscillating evolutionary processes are likely to have played an important part in the origin of life. It is a mistake to assume that life originated in any one environment. It did not arrive in a moment of time. It was the result of a long period of chemical evolution during which it passed through a variety of environments. Biopoesis took place in an environment in which a variety of different kinds of protolife were assembled and concentrated. One essential form of protolife involved in these processes is the protocell. The experiments of Fox suggest that the creation of protocells involves violent oscillations of temperature and hydration. Igneous activity is especially characterised by oscillating conditions. Volcanic eruptions consist of violent changes from one extreme condition to another. Temperatures, pressure, phase, concentration and hydration all oscillate violently, and are subject to shock pulses of many kinds. Protolife may well have passed through extremes of environment for wider that those that life itself can sustain. The most probable environment for the assembly of the various forms of protolife would be on mudbanks forming either at the mouth of streams draining regions of active vulcanicity, or round the edge of hot volclanic pools. In this situation one could fins concentrated not only the various stands of protolife necessary for the final act of biopoesis, but also perbiologically formed nutrients necessary as for the first eobionts. As soon as the first protocells start to grow, they start to compete with each other, and so initiate a new additional evolutionary process, that of natural selection. Only after such competition has been initiated is life itself likely to be established\"20", "PMID": 967406}
{"id": "pubmed23n0032_7048", "title": "[Hypokinetic appearance of huntington's chorea during the childhood (author's transl)].", "content": "During the last two years two patients with hypokinetic appearance of Huntington's chorea--beginning in childhood--were investigated in the Neurological Univ.-Clinic of Vienna. Both of the patients descend from families with primary hyperkinetic Huntington's chorea in adult life. The problem of the early manifest hypokinetic form--when the family case history is not attended--are of special interest for the first symptoms can lead into false diagnosis. Therefore oftenly systematic treatment will be done at first just during the adult life, when already the complete picture of Huntington's chorea has appeared. From the point of eugenic deliberations it is very interesting for relatives of families with Huntington's chorea to be informed about the heredity, the most lately appearance of the disease and by the possiblity of anteposition.", "contents": "[Hypokinetic appearance of huntington's chorea during the childhood (author's transl)]. During the last two years two patients with hypokinetic appearance of Huntington's chorea--beginning in childhood--were investigated in the Neurological Univ.-Clinic of Vienna. Both of the patients descend from families with primary hyperkinetic Huntington's chorea in adult life. The problem of the early manifest hypokinetic form--when the family case history is not attended--are of special interest for the first symptoms can lead into false diagnosis. Therefore oftenly systematic treatment will be done at first just during the adult life, when already the complete picture of Huntington's chorea has appeared. From the point of eugenic deliberations it is very interesting for relatives of families with Huntington's chorea to be informed about the heredity, the most lately appearance of the disease and by the possiblity of anteposition.", "PMID": 967501}
{"id": "pubmed23n0032_7049", "title": "[Gastrostomy in paediatric surgery (author's transl)].", "content": "Gastrostomy has the same field of application as the feeding tube which is introduced either orally or nasally. With careful nursing and surgical technique, only few and slight disturbances are to be expected. This method, however, ist not suited for routine application by the premature baby because of its possible septic complications.", "contents": "[Gastrostomy in paediatric surgery (author's transl)]. Gastrostomy has the same field of application as the feeding tube which is introduced either orally or nasally. With careful nursing and surgical technique, only few and slight disturbances are to be expected. This method, however, ist not suited for routine application by the premature baby because of its possible septic complications.", "PMID": 967502}
{"id": "pubmed23n0032_7050", "title": "[Cardiac manifestations in prenatal coxsackie virus myocarditis (author's transl)].", "content": "This is a report of 6 single cases of prenatal virus myocarditis originated from an endemic region around Salzburg. The cardiac involvement showed different grades dependent too on time of infection and on transfer of maternal antibodies to the child. Infections immediately before delivery cause severe illness, but infections some weeks before birth less severe damage. Symptoms of meningitis were not noticed. The diagnosis of myocarditis by Coxsackie virus was certain in two deceased newborns (Patient 1 and 2), very probable in one decreased baby (Patient 3) and probable in the surviving children (Patient 4, 5 and 6).", "contents": "[Cardiac manifestations in prenatal coxsackie virus myocarditis (author's transl)]. This is a report of 6 single cases of prenatal virus myocarditis originated from an endemic region around Salzburg. The cardiac involvement showed different grades dependent too on time of infection and on transfer of maternal antibodies to the child. Infections immediately before delivery cause severe illness, but infections some weeks before birth less severe damage. Symptoms of meningitis were not noticed. The diagnosis of myocarditis by Coxsackie virus was certain in two deceased newborns (Patient 1 and 2), very probable in one decreased baby (Patient 3) and probable in the surviving children (Patient 4, 5 and 6).", "PMID": 967503}
{"id": "pubmed23n0032_7051", "title": "[Cystic changes in the lungs in childhood (author's transl)].", "content": "Two boys with presumably congenital cystic changes in the lungs are described. In one child multiple cystic deformations led to acute symptoms of severe respiratory distress necessitating urgent resection of the right middle and upper lobes. The other boy had a large thick-walled cyst in the left upper lobe causing no distress whatsoever. This patient was observed for more than 6 years before operation. Multiple vascular anomalies and anastomoses were revealed during surgery. Both cases are presented in detail and the completely different clinical course is outlined. Only when careful supervision of intrapulmonary cystic changes is guaranteed can operation be postponed for longer than 6 months. Pulmonary cysts may lead to emergency procedures. They are always a potential source of serious complications such as rupture or infection. When no tendency for regression can be discovered surgical removal is indicated. Conservative treatment will not influence the course. Differential diagnosis is discussed briefly.", "contents": "[Cystic changes in the lungs in childhood (author's transl)]. Two boys with presumably congenital cystic changes in the lungs are described. In one child multiple cystic deformations led to acute symptoms of severe respiratory distress necessitating urgent resection of the right middle and upper lobes. The other boy had a large thick-walled cyst in the left upper lobe causing no distress whatsoever. This patient was observed for more than 6 years before operation. Multiple vascular anomalies and anastomoses were revealed during surgery. Both cases are presented in detail and the completely different clinical course is outlined. Only when careful supervision of intrapulmonary cystic changes is guaranteed can operation be postponed for longer than 6 months. Pulmonary cysts may lead to emergency procedures. They are always a potential source of serious complications such as rupture or infection. When no tendency for regression can be discovered surgical removal is indicated. Conservative treatment will not influence the course. Differential diagnosis is discussed briefly.", "PMID": 967504}
{"id": "pubmed23n0032_7052", "title": "[Diagnosis and therapy of chronic constipation].", "content": "Severe chronic constipation is an extremely rare disorder. Out of 39 370 patients seen in our hospital during the past two years only 309 children (=0,78%) were suffered from severe chronic constipation. Radiologic and electromanometrical examinations revealed as an organic cause anal sphincter achalasia or Hirschsprung's disease in 30,7% of these constipated children. In 54,9% however no pathomorphologic results could be found. Of all cases 14,4% examined were postoperative controls. Careful electromanometric and defecographic examination of every chronically constipated child is crucially important in determining the therapy as in most cases chronic constipation in childhood, is due to a functional asynchronism of the internal and external anal sphincter relaxation reflexes, it can most reliably be diagnosed by electromanometrical examinations. The accuracy and reliability of our electromanometrical method has been proved not only by comparison with radiologic and histologic techniques but also statistically by analysis of discriminance of the recorded parameters. The therapeutic approach depends on the underlying disease. Mechanical obstruction of the intestine, nervous dysregulation of colonic motility causing Hirschsprung's disease and organic anal sphincter achalasia require surgical treatment. In cases of achalasia myotomia of the internal anal sphincter gives excellent functional results. In 73 cases treated by myotomy no symptoms of anal incontinence were seen. Medical therapy includes administration of laxatives combined with high roughage diet, adjustment of living conditions including increased physical activity and bowel training in order to learn a conditioned defecation reflex. The matter can be more easily achieved by administering Dihydroergotamine and Lactulose initially.", "contents": "[Diagnosis and therapy of chronic constipation]. Severe chronic constipation is an extremely rare disorder. Out of 39 370 patients seen in our hospital during the past two years only 309 children (=0,78%) were suffered from severe chronic constipation. Radiologic and electromanometrical examinations revealed as an organic cause anal sphincter achalasia or Hirschsprung's disease in 30,7% of these constipated children. In 54,9% however no pathomorphologic results could be found. Of all cases 14,4% examined were postoperative controls. Careful electromanometric and defecographic examination of every chronically constipated child is crucially important in determining the therapy as in most cases chronic constipation in childhood, is due to a functional asynchronism of the internal and external anal sphincter relaxation reflexes, it can most reliably be diagnosed by electromanometrical examinations. The accuracy and reliability of our electromanometrical method has been proved not only by comparison with radiologic and histologic techniques but also statistically by analysis of discriminance of the recorded parameters. The therapeutic approach depends on the underlying disease. Mechanical obstruction of the intestine, nervous dysregulation of colonic motility causing Hirschsprung's disease and organic anal sphincter achalasia require surgical treatment. In cases of achalasia myotomia of the internal anal sphincter gives excellent functional results. In 73 cases treated by myotomy no symptoms of anal incontinence were seen. Medical therapy includes administration of laxatives combined with high roughage diet, adjustment of living conditions including increased physical activity and bowel training in order to learn a conditioned defecation reflex. The matter can be more easily achieved by administering Dihydroergotamine and Lactulose initially.", "PMID": 967505}
{"id": "pubmed23n0032_7053", "title": "[Medullary sponge kidney: a short review and a report of two cases (author's transl)].", "content": "Radiologically the collecting tubules in the medulla are dilated up to cystic proportions. There may be no clinical symptoms, but frequently the dilated tubules predispose to infection and lithiasis. Such cysts do not interfere with renal function. X-ray diagnosis, frequency, therapy and etiology are discussed. Two further cases of MSK in childhood are described.", "contents": "[Medullary sponge kidney: a short review and a report of two cases (author's transl)]. Radiologically the collecting tubules in the medulla are dilated up to cystic proportions. There may be no clinical symptoms, but frequently the dilated tubules predispose to infection and lithiasis. Such cysts do not interfere with renal function. X-ray diagnosis, frequency, therapy and etiology are discussed. Two further cases of MSK in childhood are described.", "PMID": 967506}
{"id": "pubmed23n0032_7054", "title": "[Manifestation of diabetes in the first to third year of life. Later fates of 43 patients (author's transl)].", "content": "Life expectancy of diabetics since early childhood is differently judged. Out of 43 own patients with diabetes started between the first and third year of life, no less than 33 have passed their twentieth year of life; 16 patients are aged between 25 and 46 years; one woman patient lived to the age of 64! In all cases the duration of diabetes is more than 10 years, in 26 cases 20 years or more. In 24 cases angiopathy developed chiefly in the form of retinopathy, nephroapthy and arterial hypertension (none in 19 cases). 7 patients died of uraemia. While there is hardly any difference between the two groups (with or without angiopathy) as far as duration and quality of diabetes-control are concerned, in the angiopathy-group hereditary taint clearly prevails. The early beginning of diabetes does not at all represent an absolutely unfavourable symptom, rather the reverse (O. Imerslund).", "contents": "[Manifestation of diabetes in the first to third year of life. Later fates of 43 patients (author's transl)]. Life expectancy of diabetics since early childhood is differently judged. Out of 43 own patients with diabetes started between the first and third year of life, no less than 33 have passed their twentieth year of life; 16 patients are aged between 25 and 46 years; one woman patient lived to the age of 64! In all cases the duration of diabetes is more than 10 years, in 26 cases 20 years or more. In 24 cases angiopathy developed chiefly in the form of retinopathy, nephroapthy and arterial hypertension (none in 19 cases). 7 patients died of uraemia. While there is hardly any difference between the two groups (with or without angiopathy) as far as duration and quality of diabetes-control are concerned, in the angiopathy-group hereditary taint clearly prevails. The early beginning of diabetes does not at all represent an absolutely unfavourable symptom, rather the reverse (O. Imerslund).", "PMID": 967507}
{"id": "pubmed23n0032_7055", "title": "[Secondary cystathioninuria due to vitamin B 6 deficiency in familial neuroblastoma].", "content": "In this study numerous amino acid analyses were performed in a family with increased incidence of tumors of the sympathetic nervous system. Since in 9 respectively 10 of a total of 13 surveyed persons an elevated urinary excretion of cystathionine was demonstrated with thin layer and column chromatographic methods, this constitutes the first report on cystathioninuria in familial neuroblastoma. Two family members also presented a homocystinuria in the spontaneously voided 24-hours urine. The only person whose plasma amino acids were analysed showed nearly normal levels. After oral loading with L-methionine the urinary excretion of cystathionine raised considerably. The different reaction of the two vitamin B6-dependent enzymes, cystathionine-synthetase and cystathionase, points at a different methionine induced sensitivity. The determination of pyridoxal phosphate and pyridoxal kinase detected a vitamin B6-deficiency, which corresponded well with an increased excretion of oxalic acid and a low normal urinary taurin excretion. Therefore these alterations of the amino acids are explained and thus urinary excretion of cystathionine can be interpreted as secondary cystathioninuria. Furthermore it was possible to provoke corresponding biochemical changes by oral administration of vitamin B6 such as reduction of the cystathioninuria and disappearance of the homocystinuria. In an infant the cystathioninuria could be observed over a period for 5 months, by which a transitory deficiency of the apoenzyme appears to be unlikely. The analysis of 24 hours urine samples of a gravida showed the persistance of cystathioninuria also during pregnancy. After radiation of a 5 year old girl with ganglioneuroblastoma an increase of the urinary cystathionine excretion and a first occurrence of homocystinuria was noted. These observations give rise to various considerations. The vitamin-B6-deficiency in familial neuroblastoma supports the assumption, that also the cystathioninuria in nonhereditary cases may be caused by vitamin-B6-deficiency. Since in this family the excretion of catecholamines was examined in a prior investigation a comparison of these two studies does not support the suggestion of a direct connection between the excretion of catecholamines and cystathioninuria, as it has been assumed to occur in sporadic neuroblastoma. The vitamin-B6-deficiency as seen in this family can also be considered in relation to tumor development. In the discussion about this possibility also the appearance of cystathioninuria in other tumors of early childhood is mentioned. Furthermore the relation of vitamin B6 to teratogenesis is commented on.", "contents": "[Secondary cystathioninuria due to vitamin B 6 deficiency in familial neuroblastoma]. In this study numerous amino acid analyses were performed in a family with increased incidence of tumors of the sympathetic nervous system. Since in 9 respectively 10 of a total of 13 surveyed persons an elevated urinary excretion of cystathionine was demonstrated with thin layer and column chromatographic methods, this constitutes the first report on cystathioninuria in familial neuroblastoma. Two family members also presented a homocystinuria in the spontaneously voided 24-hours urine. The only person whose plasma amino acids were analysed showed nearly normal levels. After oral loading with L-methionine the urinary excretion of cystathionine raised considerably. The different reaction of the two vitamin B6-dependent enzymes, cystathionine-synthetase and cystathionase, points at a different methionine induced sensitivity. The determination of pyridoxal phosphate and pyridoxal kinase detected a vitamin B6-deficiency, which corresponded well with an increased excretion of oxalic acid and a low normal urinary taurin excretion. Therefore these alterations of the amino acids are explained and thus urinary excretion of cystathionine can be interpreted as secondary cystathioninuria. Furthermore it was possible to provoke corresponding biochemical changes by oral administration of vitamin B6 such as reduction of the cystathioninuria and disappearance of the homocystinuria. In an infant the cystathioninuria could be observed over a period for 5 months, by which a transitory deficiency of the apoenzyme appears to be unlikely. The analysis of 24 hours urine samples of a gravida showed the persistance of cystathioninuria also during pregnancy. After radiation of a 5 year old girl with ganglioneuroblastoma an increase of the urinary cystathionine excretion and a first occurrence of homocystinuria was noted. These observations give rise to various considerations. The vitamin-B6-deficiency in familial neuroblastoma supports the assumption, that also the cystathioninuria in nonhereditary cases may be caused by vitamin-B6-deficiency. Since in this family the excretion of catecholamines was examined in a prior investigation a comparison of these two studies does not support the suggestion of a direct connection between the excretion of catecholamines and cystathioninuria, as it has been assumed to occur in sporadic neuroblastoma. The vitamin-B6-deficiency as seen in this family can also be considered in relation to tumor development. In the discussion about this possibility also the appearance of cystathioninuria in other tumors of early childhood is mentioned. Furthermore the relation of vitamin B6 to teratogenesis is commented on.", "PMID": 967508}
{"id": "pubmed23n0032_7056", "title": "[Prophylactic replacement therapy in hemophilia. A case report (author's transl)].", "content": "In a six year old boy with severe hemophilia prophylactic substitution of factor VIII was started in 1973 in order to prevent early invalidity due to series of bleeding in the right anklejoint. A substitution of factor VIII over 8 months with 21 resp. 30 U/kg body-weight did not lead to a significant improvement. But since the factor VIII in a dosage of 18 U/kg body-weight is given three times a week no bleeding occurred during the treatment time of 14 months and also the ability to walk improved to an excellent degree.--So far no signs of hepatitis or an factor-VIII-antibody could be detected.--Some results from the prophylactic treatment of severe hemophilia and Christmas disease are cited from the literature.", "contents": "[Prophylactic replacement therapy in hemophilia. A case report (author's transl)]. In a six year old boy with severe hemophilia prophylactic substitution of factor VIII was started in 1973 in order to prevent early invalidity due to series of bleeding in the right anklejoint. A substitution of factor VIII over 8 months with 21 resp. 30 U/kg body-weight did not lead to a significant improvement. But since the factor VIII in a dosage of 18 U/kg body-weight is given three times a week no bleeding occurred during the treatment time of 14 months and also the ability to walk improved to an excellent degree.--So far no signs of hepatitis or an factor-VIII-antibody could be detected.--Some results from the prophylactic treatment of severe hemophilia and Christmas disease are cited from the literature.", "PMID": 967509}
{"id": "pubmed23n0032_7057", "title": "Effect of chronic anemia on left ventricular ejection time and myocardial performance in man.", "content": "The duration of systolic time interval in ten anemic, (mean Hb 3.46) and otherwise healthy individuals (mean age 15) was measured from simultaneous recording of electrocardiogram, phonocardiogram and carotid pulsation. The preejection period (PEP); left ventricular ejection time (LVET) and ratio of PEP/LVET from 20 consecutive beats were calculated and averaged. Anemia was corrected slowly and study was repeated (mean 3 1/2 weeks) later and a similar data for comparison was obtained; heart rate decreased, LVET lengthened and the ratio of PEP/LVET remained unchanged. The lengthening of the LVET was due to slower heart rate. LVET before and after correction of anemia could be predicted from regression formula reported for normals. The unchanged PEP/LVET before and after the anemia indicates no significant alteration in myocardial performance at rest.", "contents": "Effect of chronic anemia on left ventricular ejection time and myocardial performance in man. The duration of systolic time interval in ten anemic, (mean Hb 3.46) and otherwise healthy individuals (mean age 15) was measured from simultaneous recording of electrocardiogram, phonocardiogram and carotid pulsation. The preejection period (PEP); left ventricular ejection time (LVET) and ratio of PEP/LVET from 20 consecutive beats were calculated and averaged. Anemia was corrected slowly and study was repeated (mean 3 1/2 weeks) later and a similar data for comparison was obtained; heart rate decreased, LVET lengthened and the ratio of PEP/LVET remained unchanged. The lengthening of the LVET was due to slower heart rate. LVET before and after correction of anemia could be predicted from regression formula reported for normals. The unchanged PEP/LVET before and after the anemia indicates no significant alteration in myocardial performance at rest.", "PMID": 967511}
{"id": "pubmed23n0032_7058", "title": "The range of antistreptolysin-O titer among 3129 healthy individuals in winter and summer in Tehran, Iran.", "content": "A rise in ASO titre can be demonstrated in 75-80% of patients following untreated streptococcal upper respiratory infection. The difficulty, however, is that there is no such thing as normal ASO titre. The levels encountered in a given population depend upon age, geographical location, season, etc. It was, therefore, on this basis that sera were taken from 3129 healthy individuals during winter and summer in Tehran for the determination of ASO titres. Sixty-three individuals were treated both during summer and winter. During summer only 4 (6%) had titres above 250 T.U. and 59 (94%) below 250 T.U. The same individuals, when tested during the winter, showed that 7 (11%) had ASO titres above 250 T.U. and 56 (89%) below 250 T.U. This seasonal difference is statistically significant. In another study 320 individuals were tested during summer and another 394 individuals during winter. Among the summer group 31 (10%) were above 250 T.U. and 289 (90%) were below 250 T.U. In the winter group 55 (14%) were above 250 T.U. and 339 (86%) were below 250 T.U. This difference is again statistically significant. Further, 35 samples of pooled sera representing 2289 individuals were treated during the winter. Here 563 (24%) were above and 1723 (76%) were below 250 T.U. The possible sources of error in this group, in comparison with the other, are discussed.", "contents": "The range of antistreptolysin-O titer among 3129 healthy individuals in winter and summer in Tehran, Iran. A rise in ASO titre can be demonstrated in 75-80% of patients following untreated streptococcal upper respiratory infection. The difficulty, however, is that there is no such thing as normal ASO titre. The levels encountered in a given population depend upon age, geographical location, season, etc. It was, therefore, on this basis that sera were taken from 3129 healthy individuals during winter and summer in Tehran for the determination of ASO titres. Sixty-three individuals were treated both during summer and winter. During summer only 4 (6%) had titres above 250 T.U. and 59 (94%) below 250 T.U. The same individuals, when tested during the winter, showed that 7 (11%) had ASO titres above 250 T.U. and 56 (89%) below 250 T.U. This seasonal difference is statistically significant. In another study 320 individuals were tested during summer and another 394 individuals during winter. Among the summer group 31 (10%) were above 250 T.U. and 289 (90%) were below 250 T.U. In the winter group 55 (14%) were above 250 T.U. and 339 (86%) were below 250 T.U. This difference is again statistically significant. Further, 35 samples of pooled sera representing 2289 individuals were treated during the winter. Here 563 (24%) were above and 1723 (76%) were below 250 T.U. The possible sources of error in this group, in comparison with the other, are discussed.", "PMID": 967513}
{"id": "pubmed23n0032_7059", "title": "Atmospheric pollen counts in Tehran, Iran, 1974.", "content": "Daily pollen counts were made in Tehran by placing a Durham sampler on the roof of the College of Pharmacy building at the University of Tehran. Three pollen seasons were identified in Tehran. Tree season extended from the first week of February through the middle of October. Important pollens of tree season were Willow, Cedar, Sycamore, Pine, Alder, Walnuts, Elm, Cottonwood, Oak and/or Maple, Birch, and Beech. The grass season started from the first week of May and extended into the second week of November. The weed season began at the middle of May and lasted until the last week of December. Important weed pollens belonged to various members of the Order Chenopodials, particularly Burning Bush, and Russian Thistle. Sagebrush and English Plantain were also abundant during the weed season.", "contents": "Atmospheric pollen counts in Tehran, Iran, 1974. Daily pollen counts were made in Tehran by placing a Durham sampler on the roof of the College of Pharmacy building at the University of Tehran. Three pollen seasons were identified in Tehran. Tree season extended from the first week of February through the middle of October. Important pollens of tree season were Willow, Cedar, Sycamore, Pine, Alder, Walnuts, Elm, Cottonwood, Oak and/or Maple, Birch, and Beech. The grass season started from the first week of May and extended into the second week of November. The weed season began at the middle of May and lasted until the last week of December. Important weed pollens belonged to various members of the Order Chenopodials, particularly Burning Bush, and Russian Thistle. Sagebrush and English Plantain were also abundant during the weed season.", "PMID": 967514}
{"id": "pubmed23n0032_7060", "title": "The stimulation of daughter redia production during the larval development of Fasciola hepatica.", "content": "In snails maintained at 20 degrees C rediae of Fasciola hepatica merge from sporocysts from 11 days after infection onwards. The number of mother rediae rises steadily thereafter until at least 40 days after infection. Daughter rediae are seldom observed in mother rediae dissected from snails maintained at 20 degrees C. Their production can, however, be stimulated by subjecting the snail host to starvation, to low, and to high temperature shocks. The parasite is susceptible to stress from immediately after infection for about 16 days, when maintained at 20 degrees C. In general, the more extreme the shock, the greater is daughter redial production. Increasing the length of the period of stress from 12 h up to 9 days does not increase the production of daughter rediae, nor does repeated on/off cold shocks or continuous maintenance at 10 degrees C. Daughter rediae develop more rapidly than cercariae and leave the mother rediae several days earlier. There is no evidence that presence of daughter rediae coincides with the suppression of cercarial production. The findings are discussed with reference to possible mechanisms by which parasite development might be controlled.", "contents": "The stimulation of daughter redia production during the larval development of Fasciola hepatica. In snails maintained at 20 degrees C rediae of Fasciola hepatica merge from sporocysts from 11 days after infection onwards. The number of mother rediae rises steadily thereafter until at least 40 days after infection. Daughter rediae are seldom observed in mother rediae dissected from snails maintained at 20 degrees C. Their production can, however, be stimulated by subjecting the snail host to starvation, to low, and to high temperature shocks. The parasite is susceptible to stress from immediately after infection for about 16 days, when maintained at 20 degrees C. In general, the more extreme the shock, the greater is daughter redial production. Increasing the length of the period of stress from 12 h up to 9 days does not increase the production of daughter rediae, nor does repeated on/off cold shocks or continuous maintenance at 10 degrees C. Daughter rediae develop more rapidly than cercariae and leave the mother rediae several days earlier. There is no evidence that presence of daughter rediae coincides with the suppression of cercarial production. The findings are discussed with reference to possible mechanisms by which parasite development might be controlled.", "PMID": 967517}
{"id": "pubmed23n0032_7061", "title": "The cell coat of Crithidia fasciculata.", "content": "A prominent filamentous and granular cell coat is associated with the plasma membrane of haptomonads of Crithidia fasciculata attached to the cuticular lining of the hindgut of Anopheles gambiae. This surface material is completely absent from morphologically identical haptomonads from the mosquito foregut and from rosettes in culture. On the basis of these observations, it is suggested that cell coat formation is dependent on suitable environmental conditions. A cytochemical staining technique indicates the presence of carbohydrates in (a) the cell coat, (b) the outer leaflet of the plasma membrane of haptomonads from all locations and (c) small cytoplasmic vesicles and multivesicular bodies in all haptomonads. It is suggested that these cytoplasmic organelles represent stages in the intracellular pathway for material ingested by pinocytosis rather than elements involved in surface coat formation.", "contents": "The cell coat of Crithidia fasciculata. A prominent filamentous and granular cell coat is associated with the plasma membrane of haptomonads of Crithidia fasciculata attached to the cuticular lining of the hindgut of Anopheles gambiae. This surface material is completely absent from morphologically identical haptomonads from the mosquito foregut and from rosettes in culture. On the basis of these observations, it is suggested that cell coat formation is dependent on suitable environmental conditions. A cytochemical staining technique indicates the presence of carbohydrates in (a) the cell coat, (b) the outer leaflet of the plasma membrane of haptomonads from all locations and (c) small cytoplasmic vesicles and multivesicular bodies in all haptomonads. It is suggested that these cytoplasmic organelles represent stages in the intracellular pathway for material ingested by pinocytosis rather than elements involved in surface coat formation.", "PMID": 967518}
{"id": "pubmed23n0032_7062", "title": "Partial purification of antigens collected during in vitro cultivation of the larval stages of Taenia pisiformis.", "content": "Larvae of Taenia pisiformis were cultured in vitro in medium containing 2-5, 5 or 20% (v/v) of normal rabbit serum (NRS). Greatest development occurred in 20% NRS, and the potency of antigens collected in medium from each culture tested by intradermal (2/d) skin tests in infected rabbits paralleled the in vitro growth rate of larvae. 'Culture' antigens from 5% NRS stimulated good immunity in rabbits to a challenge infection with T. pisiformis eggs, although they were poorly reactive in skin tests. T. pisiformis larvae were also cultured in 10% (v/v) of filtrates of serum reduced to one-half of its volume by passage through 300 000 MW cut off (XM300F) or 100 000 MW cut off (XM100F) ultrafiltration membranes. Larvae cultured using XM300F had growth rates comparable with those cultured in 20% NRS, and the antigens release into the culture medium had equal potency in i/d tests and in stimulating protective immunity in rabbits. Larvae did not develop in XM100F or produce skin-reactive or protective antigens. Crude 'culture' antigen from cultures in 20% NRS was separated into 4 fractions by filtration on Sephadex G200. All of these fractions gave i/d skin reactions in infected rabbits. Fraction 3 (F3) was the most active, but was shown by acrylamide gel electrophoresis and immunoelectrophoresis to be highly contaminated with rabbit serum proteins. F3 was separated into fractions on DEAE-Sephadex A50, and the third fraction from this was as active as the original culture medium in i/d skin tests, but had only 5% of the original protein concentration. Electrophoresis demonstrated few serum contaminants, and 2 indistinct protein bands that were not present in a similar fraction of NRS. Neither Sephadex G200 F3 nor DEAE-Sephadex F3 stimulated protective immunity in rabbits, suggesting that antigens stimulating immunity against the establishment of T. pisiformis in rabbits and those provoking cell-mediated immune reactions may be different.", "contents": "Partial purification of antigens collected during in vitro cultivation of the larval stages of Taenia pisiformis. Larvae of Taenia pisiformis were cultured in vitro in medium containing 2-5, 5 or 20% (v/v) of normal rabbit serum (NRS). Greatest development occurred in 20% NRS, and the potency of antigens collected in medium from each culture tested by intradermal (2/d) skin tests in infected rabbits paralleled the in vitro growth rate of larvae. 'Culture' antigens from 5% NRS stimulated good immunity in rabbits to a challenge infection with T. pisiformis eggs, although they were poorly reactive in skin tests. T. pisiformis larvae were also cultured in 10% (v/v) of filtrates of serum reduced to one-half of its volume by passage through 300 000 MW cut off (XM300F) or 100 000 MW cut off (XM100F) ultrafiltration membranes. Larvae cultured using XM300F had growth rates comparable with those cultured in 20% NRS, and the antigens release into the culture medium had equal potency in i/d tests and in stimulating protective immunity in rabbits. Larvae did not develop in XM100F or produce skin-reactive or protective antigens. Crude 'culture' antigen from cultures in 20% NRS was separated into 4 fractions by filtration on Sephadex G200. All of these fractions gave i/d skin reactions in infected rabbits. Fraction 3 (F3) was the most active, but was shown by acrylamide gel electrophoresis and immunoelectrophoresis to be highly contaminated with rabbit serum proteins. F3 was separated into fractions on DEAE-Sephadex A50, and the third fraction from this was as active as the original culture medium in i/d skin tests, but had only 5% of the original protein concentration. Electrophoresis demonstrated few serum contaminants, and 2 indistinct protein bands that were not present in a similar fraction of NRS. Neither Sephadex G200 F3 nor DEAE-Sephadex F3 stimulated protective immunity in rabbits, suggesting that antigens stimulating immunity against the establishment of T. pisiformis in rabbits and those provoking cell-mediated immune reactions may be different.", "PMID": 967519}
{"id": "pubmed23n0032_7063", "title": "Seasonal variation in the population dynamics of Caryophyllaeus laticeps.", "content": "The biological parameters which generate seasonal fluctuations in the size of populations of Caryophyllaeus laticeps are examined in detail. A mathematical model is formulated to describe the dynamics of the adult parasite within the fish host Abramis brama, and the predictions of the model are compared with observed population data. The seasonality of parasite population size is shown to be caused by the combined effects of a temperature dependent mortality rate and flucate of larval parasites. The observed overdispersed distribution of adult parasite numbers within the host population is shown to be generated by variability in feeding habits between individual members of the fish population. The theoretical population model is used as a framework for the investigation of, among other factors, the comparative influences of the immigration and death rates on the dynamics of the adult parasite, the future behaviour of the system under altered environmental conditions and the importance of chance effects in the dynamics of individual parasite populations within a single host. The stability of the dynamics of the complete life-cycle of C. laticeps is discussed in relation to the inherent biological complexity of host/helminth parasite interactions.", "contents": "Seasonal variation in the population dynamics of Caryophyllaeus laticeps. The biological parameters which generate seasonal fluctuations in the size of populations of Caryophyllaeus laticeps are examined in detail. A mathematical model is formulated to describe the dynamics of the adult parasite within the fish host Abramis brama, and the predictions of the model are compared with observed population data. The seasonality of parasite population size is shown to be caused by the combined effects of a temperature dependent mortality rate and flucate of larval parasites. The observed overdispersed distribution of adult parasite numbers within the host population is shown to be generated by variability in feeding habits between individual members of the fish population. The theoretical population model is used as a framework for the investigation of, among other factors, the comparative influences of the immigration and death rates on the dynamics of the adult parasite, the future behaviour of the system under altered environmental conditions and the importance of chance effects in the dynamics of individual parasite populations within a single host. The stability of the dynamics of the complete life-cycle of C. laticeps is discussed in relation to the inherent biological complexity of host/helminth parasite interactions.", "PMID": 967520}
{"id": "pubmed23n0032_7064", "title": "Accelerated expulsion of adult Trichinella spiralis in mice given lymphoid cells and serum from infected donors.", "content": "Immunity to the adult stage of Trichinella spiralis, assessed by an acceleration of worm expulsion, was transferred to recipient mice with mesenteric lymph node cells (MLNC) or serum taken from infected donors. Immunity was transferred most effectively by MLNC taken from donors infected for 8 days, i.e. donors actively responding to infection. Transfer of both MLNC and serum brought about a marked acceleration of worm expulsion in all cases, even where MLNC or serum given separately failed to transfer a significant degree of immunity.", "contents": "Accelerated expulsion of adult Trichinella spiralis in mice given lymphoid cells and serum from infected donors. Immunity to the adult stage of Trichinella spiralis, assessed by an acceleration of worm expulsion, was transferred to recipient mice with mesenteric lymph node cells (MLNC) or serum taken from infected donors. Immunity was transferred most effectively by MLNC taken from donors infected for 8 days, i.e. donors actively responding to infection. Transfer of both MLNC and serum brought about a marked acceleration of worm expulsion in all cases, even where MLNC or serum given separately failed to transfer a significant degree of immunity.", "PMID": 967521}
{"id": "pubmed23n0032_7065", "title": "The growth of secondary infections of Hymenolepis microstoma in mice: the effect of various primary infection regimes.", "content": "Groups of mice were infected with 1, 5 or 10 cysticercoids of Hymenolepis microstoma. Infections were terminated with the anthelmintic 'Zanil' after 5, 15 or 30 days and the mice challenged with 6 cysticercoids. The growth of the worms in the secondary infections decreased as either the intensity (number of cysticercoids administered) or duration of the primary infections increased. It is postulated that the results have an immunological basis.", "contents": "The growth of secondary infections of Hymenolepis microstoma in mice: the effect of various primary infection regimes. Groups of mice were infected with 1, 5 or 10 cysticercoids of Hymenolepis microstoma. Infections were terminated with the anthelmintic 'Zanil' after 5, 15 or 30 days and the mice challenged with 6 cysticercoids. The growth of the worms in the secondary infections decreased as either the intensity (number of cysticercoids administered) or duration of the primary infections increased. It is postulated that the results have an immunological basis.", "PMID": 967522}
{"id": "pubmed23n0032_7066", "title": "Eimeria maxima: a comparison of two laboratory strains with a fresh isolate.", "content": "Oocysts of the Weybridge and Houghton strains of Eimeria maxima and a fresh field isolate were similar and measured on average 30-9 X 22-4 mum. The Weybridge strain and the field isolate produced similar pathogenic effects in 6-week-old chickens, high doses causing 50-80% mortality and severe weight loss. The Houghton strain was slightly less pathogenic and few birds died but more oocysts were produced. With each strain, a single dose gave complete immunity to a light challenge with the homologous strain. Two or 3 immunizing doses gave complete immunity to a heavy homologous challenge but were not sufficient to protect against heterologous challenge.", "contents": "Eimeria maxima: a comparison of two laboratory strains with a fresh isolate. Oocysts of the Weybridge and Houghton strains of Eimeria maxima and a fresh field isolate were similar and measured on average 30-9 X 22-4 mum. The Weybridge strain and the field isolate produced similar pathogenic effects in 6-week-old chickens, high doses causing 50-80% mortality and severe weight loss. The Houghton strain was slightly less pathogenic and few birds died but more oocysts were produced. With each strain, a single dose gave complete immunity to a light challenge with the homologous strain. Two or 3 immunizing doses gave complete immunity to a heavy homologous challenge but were not sufficient to protect against heterologous challenge.", "PMID": 967523}
{"id": "pubmed23n0032_7067", "title": "Milk-borne infection of rats with Strongyloides ratti and Nippostrongylus brasiliensis.", "content": "The numbers of mature worms which developed in young rats after their mothers were injected with 4000 L3 late in lactation were 1% (Nippostrongylus brasiliensis) and 24% (Strongyloides ratti) of the dose administered. The low value for N. brasiliensis validates the conclusion that milk is a real and important vehicle for infection in S. ratti since possible errors from skin invasion of the young would have been common to both species. The level of mature S. ratti infection in lactating mothers in this experiment was negligible, 97-99% of the adult worms appearing in the offspring. These results may indicate that the milk route is possible with N. brasiliensis even though it is quantitatively insignificant.", "contents": "Milk-borne infection of rats with Strongyloides ratti and Nippostrongylus brasiliensis. The numbers of mature worms which developed in young rats after their mothers were injected with 4000 L3 late in lactation were 1% (Nippostrongylus brasiliensis) and 24% (Strongyloides ratti) of the dose administered. The low value for N. brasiliensis validates the conclusion that milk is a real and important vehicle for infection in S. ratti since possible errors from skin invasion of the young would have been common to both species. The level of mature S. ratti infection in lactating mothers in this experiment was negligible, 97-99% of the adult worms appearing in the offspring. These results may indicate that the milk route is possible with N. brasiliensis even though it is quantitatively insignificant.", "PMID": 967524}
{"id": "pubmed23n0032_7068", "title": "The isolation and characterization of a Babesia from red deer (Cervus elaphus).", "content": "On three occasions, antibody positive blood from wild red deer produced overt infections with Babesia when inoculated into splenectomized red deer. One of the deer also became infected with Eperythrozoon sp. Babesia divergens, B. capreoli and the Babesia of red deer are morphologically similar and the marginal position of the parasites in the host cell is characteristic. Babesia were not seen and no antibody was formed in five out of six splenectomized bovine calves which were injected with parasitaemic red deer blood. Two of these calves when challenged with B. divergens were fully susceptible. A transient infection with the deer Babesia may have occurred in the sixth calf since antibody was detected and the animal resisted challenge with B. divergens. In indirect fluorescent antibody tests there was little or no difference in the titre of sera from naturally or experimentally infected cattle and deer when reacted with B. divergens or the red deer Babesia antigens. Despite their similarities, specific status for B. divergens and the red deer Babesia is probably justified; at present there is insufficient evidence to justify separation of the red deer Babesia from B. capreoli.", "contents": "The isolation and characterization of a Babesia from red deer (Cervus elaphus). On three occasions, antibody positive blood from wild red deer produced overt infections with Babesia when inoculated into splenectomized red deer. One of the deer also became infected with Eperythrozoon sp. Babesia divergens, B. capreoli and the Babesia of red deer are morphologically similar and the marginal position of the parasites in the host cell is characteristic. Babesia were not seen and no antibody was formed in five out of six splenectomized bovine calves which were injected with parasitaemic red deer blood. Two of these calves when challenged with B. divergens were fully susceptible. A transient infection with the deer Babesia may have occurred in the sixth calf since antibody was detected and the animal resisted challenge with B. divergens. In indirect fluorescent antibody tests there was little or no difference in the titre of sera from naturally or experimentally infected cattle and deer when reacted with B. divergens or the red deer Babesia antigens. Despite their similarities, specific status for B. divergens and the red deer Babesia is probably justified; at present there is insufficient evidence to justify separation of the red deer Babesia from B. capreoli.", "PMID": 967525}
{"id": "pubmed23n0032_7069", "title": "Fine structural observations on oocyte development in monogeneans.", "content": "The ultrastructural changes accompanying oocyte differentiation in the ovaries of the monogeneans, Diclidophora merlangi, Diplozoon paradoxum and Calicotyle kr\u00f6yeri have been described. In each case, oogenesis in the ovary proceeds as far as meiotic prophase in the primary oocyte. A three-stage sequence of development based on oocyte morphology is proposed: (1) Oogonia and early, immature primary oocytes are typically undifferentiated, with chromatin-laden nuclei occupying most of the cell volume. The cytoplasm contains small clumps of mitochondria and unattached ribosomal aggregates. There is evidence of mitosis and, in later stages, meiotic prophase is indicated by the appearance of nuclear synaptonemal complexes. (2) Maturing primary oocytes are characterized by increased nucleolar volume associated with the production of RNA for export to the cytoplasm. An organized GER and Golgi apparatus are established and involved in the synthesis and packaging of membrane-limited cortical granules. Annulate lamellae and nucleolus-like bodies appear in the cytoplasm and, with development, the cells increase in size and, peripherally, become interdigitated. (3) Mature primary oocytes represent a resting phase when cellular activity is minimal. Golgi disappear and the ER fragments or becomes reduced in dimensions. Mitochondria and free ribosomes are numerous and cortical granules move to the cell periphery. The cells separate and, when mature, are released from the ovary. There are minor species differences in oocyte ultrastructure and development.", "contents": "Fine structural observations on oocyte development in monogeneans. The ultrastructural changes accompanying oocyte differentiation in the ovaries of the monogeneans, Diclidophora merlangi, Diplozoon paradoxum and Calicotyle kr\u00f6yeri have been described. In each case, oogenesis in the ovary proceeds as far as meiotic prophase in the primary oocyte. A three-stage sequence of development based on oocyte morphology is proposed: (1) Oogonia and early, immature primary oocytes are typically undifferentiated, with chromatin-laden nuclei occupying most of the cell volume. The cytoplasm contains small clumps of mitochondria and unattached ribosomal aggregates. There is evidence of mitosis and, in later stages, meiotic prophase is indicated by the appearance of nuclear synaptonemal complexes. (2) Maturing primary oocytes are characterized by increased nucleolar volume associated with the production of RNA for export to the cytoplasm. An organized GER and Golgi apparatus are established and involved in the synthesis and packaging of membrane-limited cortical granules. Annulate lamellae and nucleolus-like bodies appear in the cytoplasm and, with development, the cells increase in size and, peripherally, become interdigitated. (3) Mature primary oocytes represent a resting phase when cellular activity is minimal. Golgi disappear and the ER fragments or becomes reduced in dimensions. Mitochondria and free ribosomes are numerous and cortical granules move to the cell periphery. The cells separate and, when mature, are released from the ovary. There are minor species differences in oocyte ultrastructure and development.", "PMID": 967526}
{"id": "pubmed23n0032_7070", "title": "Immunity to coccidiosis: stages of the life-cycle of Eimeria maxima which induce, and are affected by, the response of the host.", "content": "An attempt was made to determine the relative importance of the different life-cycle stages of Eimeria maxima in the induction of immunity and also those stages most affected by the immune response of the host. In one experiment the life-cycle was controlled by chemotherapy but in all other experiments partial life-cycles were induced by transfers of infected mucosa between hosts. The results indicated that the second generation schizont stage is probably that most concerned in the induction of protective immunity and that sexual stages are most susceptible to immune inhibition. After initial inhibition in the immune host the earlier asexual stages were able to resume development when transferred to a susceptible host. The longer the period of exposure to the immune environment, the less able was the parasite to recover.", "contents": "Immunity to coccidiosis: stages of the life-cycle of Eimeria maxima which induce, and are affected by, the response of the host. An attempt was made to determine the relative importance of the different life-cycle stages of Eimeria maxima in the induction of immunity and also those stages most affected by the immune response of the host. In one experiment the life-cycle was controlled by chemotherapy but in all other experiments partial life-cycles were induced by transfers of infected mucosa between hosts. The results indicated that the second generation schizont stage is probably that most concerned in the induction of protective immunity and that sexual stages are most susceptible to immune inhibition. After initial inhibition in the immune host the earlier asexual stages were able to resume development when transferred to a susceptible host. The longer the period of exposure to the immune environment, the less able was the parasite to recover.", "PMID": 967527}
{"id": "pubmed23n0032_7071", "title": "Different levels of acquired immunity to Schistosoma mansoni in two strains of hamster.", "content": "A primary infection with schistosoma mansoni, of 5 or more weeks duration, stimulated a high level of resistance to a challenge infection in the WO outbred strain of golden hamster. In sharp contrast, the LGN strain showed no statistically significant immunity to reinfection in most experiments and where detected the level of resistance was only about half that observed in the WO strain. Immunity to reinfection was assayed with the lung recovery method which assesses resistance a few days after challenge and with the conventional perfusion assay which measures immunity 6 weeks after challenge. Wide differences in the immune response to S. mansoni in different strains of the same host species have not been reported previously.", "contents": "Different levels of acquired immunity to Schistosoma mansoni in two strains of hamster. A primary infection with schistosoma mansoni, of 5 or more weeks duration, stimulated a high level of resistance to a challenge infection in the WO outbred strain of golden hamster. In sharp contrast, the LGN strain showed no statistically significant immunity to reinfection in most experiments and where detected the level of resistance was only about half that observed in the WO strain. Immunity to reinfection was assayed with the lung recovery method which assesses resistance a few days after challenge and with the conventional perfusion assay which measures immunity 6 weeks after challenge. Wide differences in the immune response to S. mansoni in different strains of the same host species have not been reported previously.", "PMID": 967528}
{"id": "pubmed23n0032_7072", "title": "Cross-immunity to Schistosoma mansoni and S. haematobium in the hamster.", "content": "Hamsters (WO strain) with a primary infection of Schistosoma mansoni or S. haematobium rapidly developed immunity to homologous challenge judged by the lung recovery assay. Immunity was detected at 4-5 weeks and reached a plateau 6 weeks after infection. Using this information, hamsters with an 8-week primary infection with S. mansoni or S. haematobium were tested for resistance to homologous reinfection and resistance to a challenge with the other species of schistosome. Primary infection with S. mansoni or S. haematobium conferred a high level of immunity to reinfection with either species of schistosome judged by the perfusion assay, involving recovery of adult worms 6-10 weeks following challenge. Estimation of the level of immunity with the lung recovery assay, 5 days after challenge, indicated that immunity due to a primary infection with S. mansoni acted at or before migration of the challenge through the lungs but immunity stimulated by a primary S. haematobium infection was only partially effective at the lung stage and substantial destruction of challenging organisms occurred at a later stage of development. Antibodies in immune sera of hamsters with a primary S. mansoni or  or S. haematobium infection were shown to bind to common antigens on the surface of young  schistosomula of either species by u.v. microscopy using as detecting agent a fluorescein-labelled rabbit antiserum directed against hamster globulins.", "contents": "Cross-immunity to Schistosoma mansoni and S. haematobium in the hamster. Hamsters (WO strain) with a primary infection of Schistosoma mansoni or S. haematobium rapidly developed immunity to homologous challenge judged by the lung recovery assay. Immunity was detected at 4-5 weeks and reached a plateau 6 weeks after infection. Using this information, hamsters with an 8-week primary infection with S. mansoni or S. haematobium were tested for resistance to homologous reinfection and resistance to a challenge with the other species of schistosome. Primary infection with S. mansoni or S. haematobium conferred a high level of immunity to reinfection with either species of schistosome judged by the perfusion assay, involving recovery of adult worms 6-10 weeks following challenge. Estimation of the level of immunity with the lung recovery assay, 5 days after challenge, indicated that immunity due to a primary infection with S. mansoni acted at or before migration of the challenge through the lungs but immunity stimulated by a primary S. haematobium infection was only partially effective at the lung stage and substantial destruction of challenging organisms occurred at a later stage of development. Antibodies in immune sera of hamsters with a primary S. mansoni or  or S. haematobium infection were shown to bind to common antigens on the surface of young  schistosomula of either species by u.v. microscopy using as detecting agent a fluorescein-labelled rabbit antiserum directed against hamster globulins.", "PMID": 967529}
{"id": "pubmed23n0032_7073", "title": "The number and size of ovarian balls of Moniliformis (Acanthocephala) from laboratory rats.", "content": "By means of conventional light microscopical and counting procedures, estimations have been made of the average number and size of ovarian balls from unfertilized and fertilized female Moniliformis dubius. The observations were made on worms recovered from experimental primary infections lasting 1, 2, 3, 4, 5, 8, 11, 14, 17 and 20 weeks in male rats. The number of ovarian balls rises to about 6300 per worm, with the maximum being attained more rapidly in unfertilized than in fertilized females. On average more ovarian balls were present in unfertilized than in fertilized females, but a marked decline in number occurred in both groups of worms towards the end of the experimental period. Similarly, on the basis of the area within the elliptical profiles of fixed ovarian balls, it appeared that those from unfertilized females were generally larger on average than those from fertilized worms.", "contents": "The number and size of ovarian balls of Moniliformis (Acanthocephala) from laboratory rats. By means of conventional light microscopical and counting procedures, estimations have been made of the average number and size of ovarian balls from unfertilized and fertilized female Moniliformis dubius. The observations were made on worms recovered from experimental primary infections lasting 1, 2, 3, 4, 5, 8, 11, 14, 17 and 20 weeks in male rats. The number of ovarian balls rises to about 6300 per worm, with the maximum being attained more rapidly in unfertilized than in fertilized females. On average more ovarian balls were present in unfertilized than in fertilized females, but a marked decline in number occurred in both groups of worms towards the end of the experimental period. Similarly, on the basis of the area within the elliptical profiles of fixed ovarian balls, it appeared that those from unfertilized females were generally larger on average than those from fertilized worms.", "PMID": 967530}
{"id": "pubmed23n0032_7074", "title": "Molecular structure and morphology of glycogen isolated from the cestode, Moniezia expansa.", "content": "A particulate polysaccharide was isolated by differential centrifugation and alkali extraction from homogenates of the cestode Moniezia expansa. The polysaccharide had the structure of a glycogen. Its chemical properties, infra-red spectrum and optical rotation showed that it consisted of alpha-1,4- and alpha-1,6-linked glucopyranose units. Examination of the complex with iodine and the precipitate with concanavalin-A showed that the structure was highly branched. Oxidation with periodate and hydrolysis with alpha- and beta-amylase were used to measure mean chain lengths. For the particulate preparation the average chain length was 12-9 glucose units and the exterior and interior chain lengths were 9-0 and 2-9 units respectively. The particulate preparation had a very high sedimentation constant (s20, w = 910) with a smaller component at about s20, w = 600, but the alkali extracted material had an s20, w = 61 similar to that shown by alkali degradation of the particulate preparation. The morphology of the particulate material was similar to that of rat liver glycogen, alpha, beta- and possible gamma-particles being identified by electron microscopy. The alpha-particles were relatively stable under acidic conditions remaining intact down to pH 2-5. At pH 1-7 the alpha-particles dissociated into their constituent beta-particles with a consequent decrease in the opalescence of the solution. The nitrogen content of 0-9% was high for a glycogen.", "contents": "Molecular structure and morphology of glycogen isolated from the cestode, Moniezia expansa. A particulate polysaccharide was isolated by differential centrifugation and alkali extraction from homogenates of the cestode Moniezia expansa. The polysaccharide had the structure of a glycogen. Its chemical properties, infra-red spectrum and optical rotation showed that it consisted of alpha-1,4- and alpha-1,6-linked glucopyranose units. Examination of the complex with iodine and the precipitate with concanavalin-A showed that the structure was highly branched. Oxidation with periodate and hydrolysis with alpha- and beta-amylase were used to measure mean chain lengths. For the particulate preparation the average chain length was 12-9 glucose units and the exterior and interior chain lengths were 9-0 and 2-9 units respectively. The particulate preparation had a very high sedimentation constant (s20, w = 910) with a smaller component at about s20, w = 600, but the alkali extracted material had an s20, w = 61 similar to that shown by alkali degradation of the particulate preparation. The morphology of the particulate material was similar to that of rat liver glycogen, alpha, beta- and possible gamma-particles being identified by electron microscopy. The alpha-particles were relatively stable under acidic conditions remaining intact down to pH 2-5. At pH 1-7 the alpha-particles dissociated into their constituent beta-particles with a consequent decrease in the opalescence of the solution. The nitrogen content of 0-9% was high for a glycogen.", "PMID": 967531}
{"id": "pubmed23n0032_7075", "title": "Evasion of the haemocytic defence reaction of certain insects by larvae of Hymenolepis diminuta (Cestoda).", "content": "Larvae of Hymenolepis diminuta develop in the haemocoele of the beetles Tribolium and Tenebrio, and of the locust Schistocerca gregaria, without being encapsulated by haemocytes. The mechanism of this evasion of the haemocytic defence reaction has been examined using various techniques. Larvae grown in culture and injected into S. gregaria have a few or no haemocytes adherent even after 8h, although latex beads injected at the same time have been thickly encapsulated. This, and results of transplanting cysticercoids and host tissue between different insect species, suggests that the surface of the larvae may bear an inherent similarity to the surface of host tissues and thus escape recognition as 'not-self' by the host's haemocytes.", "contents": "Evasion of the haemocytic defence reaction of certain insects by larvae of Hymenolepis diminuta (Cestoda). Larvae of Hymenolepis diminuta develop in the haemocoele of the beetles Tribolium and Tenebrio, and of the locust Schistocerca gregaria, without being encapsulated by haemocytes. The mechanism of this evasion of the haemocytic defence reaction has been examined using various techniques. Larvae grown in culture and injected into S. gregaria have a few or no haemocytes adherent even after 8h, although latex beads injected at the same time have been thickly encapsulated. This, and results of transplanting cysticercoids and host tissue between different insect species, suggests that the surface of the larvae may bear an inherent similarity to the surface of host tissues and thus escape recognition as 'not-self' by the host's haemocytes.", "PMID": 967532}
{"id": "pubmed23n0032_7076", "title": "Symphalangism with metacarpophalangeal fusions and elbow abnormalities.", "content": "Three generations of a family manifest similar skeletal abnormalities: proximal symphalangism with several unusual features, metacarpophalangeal synostoses, massive tarsal and carpal fusions and abnormalities of the elbows (radial head dislocation, radiohumeral synostosis). Two members of this family were previously reported by Pearlman et al. as examples of the Nievergelt syndrome, but the affected individuals lack the mesomelic dysplasia that is an integral part of that entity. Moreover, the Nievergelt syndrome does not include symphalangism. The term \"Nievergelt-Pearlman syndrome\" should be dropped.", "contents": "Symphalangism with metacarpophalangeal fusions and elbow abnormalities. Three generations of a family manifest similar skeletal abnormalities: proximal symphalangism with several unusual features, metacarpophalangeal synostoses, massive tarsal and carpal fusions and abnormalities of the elbows (radial head dislocation, radiohumeral synostosis). Two members of this family were previously reported by Pearlman et al. as examples of the Nievergelt syndrome, but the affected individuals lack the mesomelic dysplasia that is an integral part of that entity. Moreover, the Nievergelt syndrome does not include symphalangism. The term \"Nievergelt-Pearlman syndrome\" should be dropped.", "PMID": 967566}
{"id": "pubmed23n0032_7077", "title": "Giant idiopathic thymomegaly.", "content": "Apart from the anterior mediastinal position and the usual size of the normal thymus, aberrant locations and extreme size variations may occur. This is illustrated by an eight month old boy whose histologically normal thymus presented as a large mass in the right hemithorax. It is the largest normal thymus on record in an infant. Thus, idiopathic thymomegaly should also be considered in the differential diagnosis of peripheral intrathoracic mass lesions.", "contents": "Giant idiopathic thymomegaly. Apart from the anterior mediastinal position and the usual size of the normal thymus, aberrant locations and extreme size variations may occur. This is illustrated by an eight month old boy whose histologically normal thymus presented as a large mass in the right hemithorax. It is the largest normal thymus on record in an infant. Thus, idiopathic thymomegaly should also be considered in the differential diagnosis of peripheral intrathoracic mass lesions.", "PMID": 967568}
{"id": "pubmed23n0032_7078", "title": "Traumatic para-mediastinal air cyst. A case report.", "content": "The roentgenographic features of a paramediastinal air cyst following chest trauma are reported. After an initial period of enlargement the lesion completely resolved in one month. Experience with this patient and a review of similar cases reported in the literature suggests that lesions of this type may be observed for spontaneous resolution.", "contents": "Traumatic para-mediastinal air cyst. A case report. The roentgenographic features of a paramediastinal air cyst following chest trauma are reported. After an initial period of enlargement the lesion completely resolved in one month. Experience with this patient and a review of similar cases reported in the literature suggests that lesions of this type may be observed for spontaneous resolution.", "PMID": 967569}
{"id": "pubmed23n0032_7079", "title": "Epiphyseo-metaphyseal cupping following infantile scurvy.", "content": "A five year old girl presented with short femora, cupped distal metaphyses and flexion deformity of the knees suggesting previous epiphyseo-metaphyseal damage. The aetiology was suggested by finding a history of previous infantile scurvy with separated distal femoral epiphyses. The rarity of this complication and possible contributing factors are discussed.", "contents": "Epiphyseo-metaphyseal cupping following infantile scurvy. A five year old girl presented with short femora, cupped distal metaphyses and flexion deformity of the knees suggesting previous epiphyseo-metaphyseal damage. The aetiology was suggested by finding a history of previous infantile scurvy with separated distal femoral epiphyses. The rarity of this complication and possible contributing factors are discussed.", "PMID": 967570}
{"id": "pubmed23n0032_7080", "title": "The neuro-radiological examination of endocrine disorders of central origin in the child (precocious puberty, hypopituitarism).", "content": "The neuro-radiological findings in 38 cases of precocious puberty of central origin and 9 cases of hypopituitarism (craniopharyngiomas excepted), are reported. The radiological examination consisted of plain films of the skull and pneumo-encephalography. In the 9 cases with hypopituitarism radiological examination was normal in 4 and localised but quite diverse anomalies were discovered in 5. Out of 38 patients presenting with isosexual precocious puberty, 29 were female and 9 male. Out of the 29 girls, neuro-radiological examination was normal in 20 and showed a hypothalamic anomaly in 9. Out of the 9 boys, 8 had a hypothalamic anomaly, and only one examination was normal. In precocious puberty we found 1 ectopic pinealoma and 2 gliomas of the chiasma. In these three cases the clinical context and radiological examination made the diagnosis obvious. Masses were discovered in 7 (3 spongioblastomas and 4 heterotopias). In 2 cases (spongioblastomas) neurological symptoms were present and made an operation mandatory. In 5 cases (1 spongioblastoma, 4 heterotopias) precocious puberty was an isolated finding. It was not possible to make, on a clinical or radiological bases, a distinction between spongioblastoma and heterotopia. As time has passed the role of surgery has changed. Formerly, surgery aimed at excision of the lesion, but with advances in medical treatment surgical intervention is now directed towards biopsy and the histological study of lesions which may be treated by radiotherapy.", "contents": "The neuro-radiological examination of endocrine disorders of central origin in the child (precocious puberty, hypopituitarism). The neuro-radiological findings in 38 cases of precocious puberty of central origin and 9 cases of hypopituitarism (craniopharyngiomas excepted), are reported. The radiological examination consisted of plain films of the skull and pneumo-encephalography. In the 9 cases with hypopituitarism radiological examination was normal in 4 and localised but quite diverse anomalies were discovered in 5. Out of 38 patients presenting with isosexual precocious puberty, 29 were female and 9 male. Out of the 29 girls, neuro-radiological examination was normal in 20 and showed a hypothalamic anomaly in 9. Out of the 9 boys, 8 had a hypothalamic anomaly, and only one examination was normal. In precocious puberty we found 1 ectopic pinealoma and 2 gliomas of the chiasma. In these three cases the clinical context and radiological examination made the diagnosis obvious. Masses were discovered in 7 (3 spongioblastomas and 4 heterotopias). In 2 cases (spongioblastomas) neurological symptoms were present and made an operation mandatory. In 5 cases (1 spongioblastoma, 4 heterotopias) precocious puberty was an isolated finding. It was not possible to make, on a clinical or radiological bases, a distinction between spongioblastoma and heterotopia. As time has passed the role of surgery has changed. Formerly, surgery aimed at excision of the lesion, but with advances in medical treatment surgical intervention is now directed towards biopsy and the histological study of lesions which may be treated by radiotherapy.", "PMID": 967572}
{"id": "pubmed23n0032_7081", "title": "Arteriographic diagnosis of brain death in children.", "content": "Transfemoral cerebral angiography was performed in four children with brain death secondary to increased intracranial pressure. Findings not previously described are filling of the cavernous, petrous and cervical portions of the contralateral internal carotid artery via the anterior communicating artery, filling of the basilar and vertebral arteries via the posterior communicating artery and anterior displacement of the basilar artery.", "contents": "Arteriographic diagnosis of brain death in children. Transfemoral cerebral angiography was performed in four children with brain death secondary to increased intracranial pressure. Findings not previously described are filling of the cavernous, petrous and cervical portions of the contralateral internal carotid artery via the anterior communicating artery, filling of the basilar and vertebral arteries via the posterior communicating artery and anterior displacement of the basilar artery.", "PMID": 967573}
{"id": "pubmed23n0032_7082", "title": "Radiographic findings in children with psychosocial dwarfism.", "content": "The diagnosis of psychosocial dwarfism is established through history, the determination of delay in growth and skeletal maturation, the lack of evidence of organic disease and accelerated growth and psychomotor development in a nuturing environment. Radiographically temporary widening of the cranial sutures may be detected in a follow up examination or less often already at the time of initial examination. Determination of wide cranial sutures may be contributary in establishing the diagnosis of psychosocial dwarfism. In a patient with this syndrome and lack of neurological signs or symptoms widening of the cranial sutures is an expected finding and no further neuroradiologic studies are necessary.", "contents": "Radiographic findings in children with psychosocial dwarfism. The diagnosis of psychosocial dwarfism is established through history, the determination of delay in growth and skeletal maturation, the lack of evidence of organic disease and accelerated growth and psychomotor development in a nuturing environment. Radiographically temporary widening of the cranial sutures may be detected in a follow up examination or less often already at the time of initial examination. Determination of wide cranial sutures may be contributary in establishing the diagnosis of psychosocial dwarfism. In a patient with this syndrome and lack of neurological signs or symptoms widening of the cranial sutures is an expected finding and no further neuroradiologic studies are necessary.", "PMID": 967574}
{"id": "pubmed23n0032_7083", "title": "Radiologic features of \"adult type\" polycystic kidney disease in the neonate.", "content": "Two cases are reported of adult type polycystic renal disease (autosomal dominant) presenting in the newborn as a unilateral abdominal mass. The radiographic findings in the involved kidney simulated the ectatic tubules of infantile polycystic disease, yet histologic examination was consistent with the adult variety and both infants had other family members with adult type polycystic kidneys. These cases emphasize some of the ambiguities that exist in the definition and classification of polycystic renal disease.", "contents": "Radiologic features of \"adult type\" polycystic kidney disease in the neonate. Two cases are reported of adult type polycystic renal disease (autosomal dominant) presenting in the newborn as a unilateral abdominal mass. The radiographic findings in the involved kidney simulated the ectatic tubules of infantile polycystic disease, yet histologic examination was consistent with the adult variety and both infants had other family members with adult type polycystic kidneys. These cases emphasize some of the ambiguities that exist in the definition and classification of polycystic renal disease.", "PMID": 967575}
{"id": "pubmed23n0032_7084", "title": "Multiple bladder filling defects in children with cystitis.", "content": "Two children with cystitis presented with multiple filling defects in the urinary bladder. They were proven to be cases of cystitis glandularis with proliferative changes of the bladder epithelium. The radiographic findings are described and the differential diagnosis from neoplasms is outlined.", "contents": "Multiple bladder filling defects in children with cystitis. Two children with cystitis presented with multiple filling defects in the urinary bladder. They were proven to be cases of cystitis glandularis with proliferative changes of the bladder epithelium. The radiographic findings are described and the differential diagnosis from neoplasms is outlined.", "PMID": 967576}
{"id": "pubmed23n0032_7085", "title": "Postmortem angiocardiography in newborn infants with congenital malformation of the heart and great vessels.", "content": "In congenital malformations of the heart and great vessels with a fatal outcome a postmortem angiocardiography may contribute to diagnosis. In this technique the heart is not dissected and the topographical relationships remain unchanged. A postmorten angiocardiography is of particular interest if no autopsy is to be performed. Some patho-anatomical details are demonstrated in three selected cases: newborn infants with 1. hypoplastic left heart syndrome, 2. truncus arteriosus communis with interruption of the aortic arch, and 3. aortic-left ventricular tunnel.", "contents": "Postmortem angiocardiography in newborn infants with congenital malformation of the heart and great vessels. In congenital malformations of the heart and great vessels with a fatal outcome a postmortem angiocardiography may contribute to diagnosis. In this technique the heart is not dissected and the topographical relationships remain unchanged. A postmorten angiocardiography is of particular interest if no autopsy is to be performed. Some patho-anatomical details are demonstrated in three selected cases: newborn infants with 1. hypoplastic left heart syndrome, 2. truncus arteriosus communis with interruption of the aortic arch, and 3. aortic-left ventricular tunnel.", "PMID": 967577}
{"id": "pubmed23n0032_7086", "title": "Experiences with triggered pulmonary exposures in children.", "content": "With conventional manual triggering technique pulmonary exposures expected to time and end-inspiration were made on 51 small children. These exposures showed a random distribution. Transthoracic impedance reflects the variations in the pulmonary air-fluid ratio during respiration. In 94 infants and small children the transthoracic impedance was monitored while pulmonary exposures were triggered by a pulse given at the level of end-inspiration or end-expiration. It was found that transthoracic impedance as a triggering device permitted precise matching of the exposures to end-inspiration or end-expiration.", "contents": "Experiences with triggered pulmonary exposures in children. With conventional manual triggering technique pulmonary exposures expected to time and end-inspiration were made on 51 small children. These exposures showed a random distribution. Transthoracic impedance reflects the variations in the pulmonary air-fluid ratio during respiration. In 94 infants and small children the transthoracic impedance was monitored while pulmonary exposures were triggered by a pulse given at the level of end-inspiration or end-expiration. It was found that transthoracic impedance as a triggering device permitted precise matching of the exposures to end-inspiration or end-expiration.", "PMID": 967578}
{"id": "pubmed23n0032_7087", "title": "Hodgkin's disease in children: clinico-roentgenologic features of the lesion in the chest.", "content": "The roentgenologic features of Hodgkin's disease were studied in 105 patients under 14 years of age. The mediastinal and hilar nodes were involved in 64.7% of patients. Some consistency of extent and pattern of disease was observed in that mediastinal lymphatic lesions could be associated with cervico-supra-clavicular and retroperitoneal lesions. There was lung infiltration in 14.3% of patients and the frequency of this lesion dependent on the histological type. It was commoner in nodular sclerosis, mixed cellularity and lymphocytic depletion. Lung infiltration usually developed in continuity with the lymphatic gland lesion and only rarely in a metastasis-like form.", "contents": "Hodgkin's disease in children: clinico-roentgenologic features of the lesion in the chest. The roentgenologic features of Hodgkin's disease were studied in 105 patients under 14 years of age. The mediastinal and hilar nodes were involved in 64.7% of patients. Some consistency of extent and pattern of disease was observed in that mediastinal lymphatic lesions could be associated with cervico-supra-clavicular and retroperitoneal lesions. There was lung infiltration in 14.3% of patients and the frequency of this lesion dependent on the histological type. It was commoner in nodular sclerosis, mixed cellularity and lymphocytic depletion. Lung infiltration usually developed in continuity with the lymphatic gland lesion and only rarely in a metastasis-like form.", "PMID": 967579}
{"id": "pubmed23n0032_7088", "title": "Preoperative diagnosis of benign hepatic hamartoma by correlation radioisotopic and angiographic studies.", "content": "The case of a 12 year old girl with a hamartoma of the liver is reported. A preoperative liver scan showed accumulation of technetium-99m sulfur colloid in the tumor with isotopic and contrast arteriography demonstration of a hypervascular intrahepatic mass. Although these diagnostic findings are not pathognomonic of hamartoma, they are indicative of a benign lesion within the liver.", "contents": "Preoperative diagnosis of benign hepatic hamartoma by correlation radioisotopic and angiographic studies. The case of a 12 year old girl with a hamartoma of the liver is reported. A preoperative liver scan showed accumulation of technetium-99m sulfur colloid in the tumor with isotopic and contrast arteriography demonstration of a hypervascular intrahepatic mass. Although these diagnostic findings are not pathognomonic of hamartoma, they are indicative of a benign lesion within the liver.", "PMID": 967580}
{"id": "pubmed23n0032_7089", "title": "The radiolucent liver.", "content": "Changes in the liver density on the plain film of the abdomen may be seen when there is a change in the nature of the liver structure. Increased amount of fat is associated with increased radioluceny which can be detected when looked for specifically. Since 1972, we have had the chance to make a diagnosis of fatty liver on the plain film of the abdomen in five patients. In all these cases, histological sections showed severe fatty change. This finding becomes easier to appreciate on the early films of an I.V.P. examination, especially when large doses are used.", "contents": "The radiolucent liver. Changes in the liver density on the plain film of the abdomen may be seen when there is a change in the nature of the liver structure. Increased amount of fat is associated with increased radioluceny which can be detected when looked for specifically. Since 1972, we have had the chance to make a diagnosis of fatty liver on the plain film of the abdomen in five patients. In all these cases, histological sections showed severe fatty change. This finding becomes easier to appreciate on the early films of an I.V.P. examination, especially when large doses are used.", "PMID": 967581}
{"id": "pubmed23n0032_7090", "title": "Magnification renal scintigraphy in the differential diagnosis of septa of Bertin.", "content": "Eight consecutive patients, referred because intravenous urography (IVP) raised the question of hypertrophied septum of Bertin versus a pathologic mass, were examined using 99mTc labeled radiopharmaceuticals with magnification renal scintigraphy. The areas of concern showed a spectrum of activity, from normal to markedly increased, as compared to surrounding tissue. The activity correlated with the size of the mass effect on IVP. Increased resolution provided by pinhole magnification was important in evaluating these patients. We feel that magnification renal scintigraphy is valuable in the evaluation of intrarenal masses.", "contents": "Magnification renal scintigraphy in the differential diagnosis of septa of Bertin. Eight consecutive patients, referred because intravenous urography (IVP) raised the question of hypertrophied septum of Bertin versus a pathologic mass, were examined using 99mTc labeled radiopharmaceuticals with magnification renal scintigraphy. The areas of concern showed a spectrum of activity, from normal to markedly increased, as compared to surrounding tissue. The activity correlated with the size of the mass effect on IVP. Increased resolution provided by pinhole magnification was important in evaluating these patients. We feel that magnification renal scintigraphy is valuable in the evaluation of intrarenal masses.", "PMID": 967582}
{"id": "pubmed23n0032_7091", "title": "Calcified thrombus in the inferior vena cava in infants and children.", "content": "Calcified thrombi in the prerenal (suprarenal) segment of the inferior vena have a characteristic radiographic appearance that permits accurate \"plain film\" diagnosis. Most have been fortuitously discovered in infants and young children. None of the affected individuals has had clinical evidence of venous obstruction. Vena caval obstruction is usually incomplete. Uncalcified clot caudal to the calcified thrombus caused complete obstruction of the inferior vena cava and renal veins in one of our patients, a healthy infant; since collateral flow was adequate surgery was not advised. We believe that aggressive diagnostic and therapeutic measures are unnecessary in the management of infants and children with calcified thrombi in the inferior vena cava.", "contents": "Calcified thrombus in the inferior vena cava in infants and children. Calcified thrombi in the prerenal (suprarenal) segment of the inferior vena have a characteristic radiographic appearance that permits accurate \"plain film\" diagnosis. Most have been fortuitously discovered in infants and young children. None of the affected individuals has had clinical evidence of venous obstruction. Vena caval obstruction is usually incomplete. Uncalcified clot caudal to the calcified thrombus caused complete obstruction of the inferior vena cava and renal veins in one of our patients, a healthy infant; since collateral flow was adequate surgery was not advised. We believe that aggressive diagnostic and therapeutic measures are unnecessary in the management of infants and children with calcified thrombi in the inferior vena cava.", "PMID": 967583}
{"id": "pubmed23n0032_7092", "title": "A form of metatropic dwarfism in two brothers.", "content": "Two brothers are described with identical features of short-limbed dwarfism, normal face, hands and feet, respiratory difficulties and mild scoliosis. It proved impossible to offer a definitive diagnosis, though radiological features as a whole bore similarities to those of metatropic dwarfism, with the most notable feature the narrowing of the ossified components of the vertebral bodies.", "contents": "A form of metatropic dwarfism in two brothers. Two brothers are described with identical features of short-limbed dwarfism, normal face, hands and feet, respiratory difficulties and mild scoliosis. It proved impossible to offer a definitive diagnosis, though radiological features as a whole bore similarities to those of metatropic dwarfism, with the most notable feature the narrowing of the ossified components of the vertebral bodies.", "PMID": 967584}
{"id": "pubmed23n0032_7093", "title": "Anomalous left pulmonary artery with an unusual barium swallow.", "content": "An atypical finding on barium swallow in a child with an anomalous left pulmonary artery causing obstructive emphysema in the right lung is described. The value of bronchography and need for early surgical intervention is stressed.", "contents": "Anomalous left pulmonary artery with an unusual barium swallow. An atypical finding on barium swallow in a child with an anomalous left pulmonary artery causing obstructive emphysema in the right lung is described. The value of bronchography and need for early surgical intervention is stressed.", "PMID": 967586}
{"id": "pubmed23n0032_7094", "title": "Cervical herniation of the lung. Report of a case and review of the literature.", "content": "Cervical herniation of the apex of the lung into the soft tissues of the neck is a rare finding in the pediatric age group. Whereas in adults the etiology is either post-traumatic or secondary to chronic respiratory disease, in children the condition appears to arise spontaneously as the result of a congenital defect in the costovertebral fascia. Surgical treatment is rarely indicated unless there is evidence of incarceration, or progressive increase in size with resultant cosmetic deformity.", "contents": "Cervical herniation of the lung. Report of a case and review of the literature. Cervical herniation of the apex of the lung into the soft tissues of the neck is a rare finding in the pediatric age group. Whereas in adults the etiology is either post-traumatic or secondary to chronic respiratory disease, in children the condition appears to arise spontaneously as the result of a congenital defect in the costovertebral fascia. Surgical treatment is rarely indicated unless there is evidence of incarceration, or progressive increase in size with resultant cosmetic deformity.", "PMID": 967587}
{"id": "pubmed23n0032_7095", "title": "Calcified subvalvular left ventricular aneurysm: an unusual case in a 4-year-old-child.", "content": "An unusual form of left ventricular aneurysm affecting young Negroes has recently been described at the aortic or mitral valve ring; constitutional factors have been postulated. A 4-year-old Negro boy was observed with a left ventricular submitral aneurysm, probably one of the largest and one of the most calcified to be reported. Tuberculous lymphadenitis involving some of the mediastinal lymph nodes was found on postmortem examination; tuberculosis was believed to be a secondary contributing factor. A review of the literature disclosed only 5 other children with calcified left ventricular aneurysms, of which 2 were of the subvalvular type.", "contents": "Calcified subvalvular left ventricular aneurysm: an unusual case in a 4-year-old-child. An unusual form of left ventricular aneurysm affecting young Negroes has recently been described at the aortic or mitral valve ring; constitutional factors have been postulated. A 4-year-old Negro boy was observed with a left ventricular submitral aneurysm, probably one of the largest and one of the most calcified to be reported. Tuberculous lymphadenitis involving some of the mediastinal lymph nodes was found on postmortem examination; tuberculosis was believed to be a secondary contributing factor. A review of the literature disclosed only 5 other children with calcified left ventricular aneurysms, of which 2 were of the subvalvular type.", "PMID": 967588}
{"id": "pubmed23n0032_7096", "title": "[Serum IgE in patients suffering from IgA deficiency with or without atopy].", "content": "Numerous studies, based upon the frequency of allergic and auto-immune disorders in association with selective deficiency in IgA, as well as the abnormally high proportion of IgA deficiency in atopic patients, have led to the hypothesis that allergy results from a deficiency in IgA during the first months of life. Thus subjects with an initial transient deficiency in IgA (followed by normal or greater than average production) produce an excess of IgE. The aim of this study, carried out by multifactorial data analysis, was to determine whether serum IgE levels were related to those of IgA. Comparison of the serum Ig levels of 4 groups of individuals, with or without IgA deficiency, showed that the production of IgE was more closely related to the respiratory condition responsible than to IgA levels. According to IgA and E levels, the 4 groups differ distinctly, atopic patients without asthma constituting a \"bridge\" between the control group and those with asthma. In the presence of an apparently identical situation, i.e. an IgA deficiency, individuals may react in two different ways: some produce an abundance of IgE whilst others fail to do so and are more susceptible to recurrent infective episodes. The role of the genetic control of IgE as well as environmental factors in the pathogenesis of allergic manifestations is discussed.", "contents": "[Serum IgE in patients suffering from IgA deficiency with or without atopy]. Numerous studies, based upon the frequency of allergic and auto-immune disorders in association with selective deficiency in IgA, as well as the abnormally high proportion of IgA deficiency in atopic patients, have led to the hypothesis that allergy results from a deficiency in IgA during the first months of life. Thus subjects with an initial transient deficiency in IgA (followed by normal or greater than average production) produce an excess of IgE. The aim of this study, carried out by multifactorial data analysis, was to determine whether serum IgE levels were related to those of IgA. Comparison of the serum Ig levels of 4 groups of individuals, with or without IgA deficiency, showed that the production of IgE was more closely related to the respiratory condition responsible than to IgA levels. According to IgA and E levels, the 4 groups differ distinctly, atopic patients without asthma constituting a \"bridge\" between the control group and those with asthma. In the presence of an apparently identical situation, i.e. an IgA deficiency, individuals may react in two different ways: some produce an abundance of IgE whilst others fail to do so and are more susceptible to recurrent infective episodes. The role of the genetic control of IgE as well as environmental factors in the pathogenesis of allergic manifestations is discussed.", "PMID": 967655}
{"id": "pubmed23n0032_7097", "title": "[Echotomography of the liver with three-dimensional representation of the organ].", "content": "Three-dimensional representation facilitates the reading of ultrasonic seriographs of the liver. It uses a conducing wire to provide a correspondence between the xipho-umbilical line and the information collected on the seriographic polaroids. Proceeding from image to image, a more thorough interpretation is possible. This analytical study provides a synthetic representation of the organ. When present lesions are seen on these diagrams and their location and dimensions clearly defined. A precise indication of the height, breadth and thickness of the organ is given, which makes it possible to obtain an accurate approximation of the hepatic volume, taking the morphotype into consideration.", "contents": "[Echotomography of the liver with three-dimensional representation of the organ]. Three-dimensional representation facilitates the reading of ultrasonic seriographs of the liver. It uses a conducing wire to provide a correspondence between the xipho-umbilical line and the information collected on the seriographic polaroids. Proceeding from image to image, a more thorough interpretation is possible. This analytical study provides a synthetic representation of the organ. When present lesions are seen on these diagrams and their location and dimensions clearly defined. A precise indication of the height, breadth and thickness of the organ is given, which makes it possible to obtain an accurate approximation of the hepatic volume, taking the morphotype into consideration.", "PMID": 967656}
{"id": "pubmed23n0032_7098", "title": "CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.", "content": "5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the change in this band were similar to the UV melting profiles of the change monitored at 260 nm. This strongly suggests a close relation between heat denaturation of the tRNA and the conformation of the S base. Oligonucleotides containing S showed negative CD bands at 320-330 nm, like the monomer S itself, but when the 3'-2/5 fragment containing S formed a complex with the complementary 5'-3/5 fragment, a positive CD band appeared at 310 nm. These results suggest that combination of the TpsiC loop containing S with the hU loop is necessary for the positive band of S at 310 nm. S may serve to strengthen the association of the TpsiC loop with the hU loop in tRNA of the thermophile.", "contents": "CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile. 5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the change in this band were similar to the UV melting profiles of the change monitored at 260 nm. This strongly suggests a close relation between heat denaturation of the tRNA and the conformation of the S base. Oligonucleotides containing S showed negative CD bands at 320-330 nm, like the monomer S itself, but when the 3'-2/5 fragment containing S formed a complex with the complementary 5'-3/5 fragment, a positive CD band appeared at 310 nm. These results suggest that combination of the TpsiC loop containing S with the hU loop is necessary for the positive band of S at 310 nm. S may serve to strengthen the association of the TpsiC loop with the hU loop in tRNA of the thermophile.", "PMID": 967669}
{"id": "pubmed23n0032_7099", "title": "Reactivity and selectivity in light-induced free radical reactions of 2-propanol with purine and pyrimidine mononucleotides and dinucleoside monophosphates.", "content": "Photoalkylation reactions with 2-propanol, initiated with di-tert-butyl peroxide, of a variety of purine and pyrimidine mononucleotides and dinucleoside monophosphates lead to the substitution of an alpha-hydroxyisopropyl group for the H-8 atom of adenosine and the addition of the alcohol across the 5,6-double bond of the pyrimidines. Adenosine moieties blocked at their 3'-hydroxyl group are alkylated faster than those blocked at their 5'-hydroxyl. The reactivity of the uridine moieties of 3'-UMP, 5'-UMP, and uridylyl-(3',5')-uridine is not affected by the location of the phosphate group. However, the uridine moiety of uridylyl-(3',5')-adenosine is modified faster than that of adenylyl-(3',5')-uridine. It is suggested that steric hindrance imposed by the phosphate group determines the reactivity of adenosine moieties, while base stacking involving adenosine determines the reactivity of uridine moieties. These two effects play a major role in controlling the nature and degree of the selectivity of these photoalkylation reactions for either adenosine or uridine. Cytidine has been found to be inert in these reactions.", "contents": "Reactivity and selectivity in light-induced free radical reactions of 2-propanol with purine and pyrimidine mononucleotides and dinucleoside monophosphates. Photoalkylation reactions with 2-propanol, initiated with di-tert-butyl peroxide, of a variety of purine and pyrimidine mononucleotides and dinucleoside monophosphates lead to the substitution of an alpha-hydroxyisopropyl group for the H-8 atom of adenosine and the addition of the alcohol across the 5,6-double bond of the pyrimidines. Adenosine moieties blocked at their 3'-hydroxyl group are alkylated faster than those blocked at their 5'-hydroxyl. The reactivity of the uridine moieties of 3'-UMP, 5'-UMP, and uridylyl-(3',5')-uridine is not affected by the location of the phosphate group. However, the uridine moiety of uridylyl-(3',5')-adenosine is modified faster than that of adenylyl-(3',5')-uridine. It is suggested that steric hindrance imposed by the phosphate group determines the reactivity of adenosine moieties, while base stacking involving adenosine determines the reactivity of uridine moieties. These two effects play a major role in controlling the nature and degree of the selectivity of these photoalkylation reactions for either adenosine or uridine. Cytidine has been found to be inert in these reactions.", "PMID": 967670}
{"id": "pubmed23n0032_7100", "title": "Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels.", "content": "A procedure is described for the detection of DNA dependent DNA and RNA polymerase activities in intact polyacrylamide gels that contain DNA. After electrophoresis under non-denaturing conditions, the intact gels are incubated with DNA or RNA polymerase reaction mixture in which one of the four deoxyribonucleoside or ribonucleoside triphosphates is radioactively labeled. The acid insoluble radioactivity associated with the intact gel is then analyzed by autoradiography of the intact gel or by liquid scintillation spectrometry of the sliced gel. Inhibition of the enzymatic activities by low molecular weight compounds such as N-ethylmaleimide or rifampin can be demonstrated by this procedure.", "contents": "Detection of DNA and RNA polymerase activities in situ following electrophoresis in polyacrylamide gels. A procedure is described for the detection of DNA dependent DNA and RNA polymerase activities in intact polyacrylamide gels that contain DNA. After electrophoresis under non-denaturing conditions, the intact gels are incubated with DNA or RNA polymerase reaction mixture in which one of the four deoxyribonucleoside or ribonucleoside triphosphates is radioactively labeled. The acid insoluble radioactivity associated with the intact gel is then analyzed by autoradiography of the intact gel or by liquid scintillation spectrometry of the sliced gel. Inhibition of the enzymatic activities by low molecular weight compounds such as N-ethylmaleimide or rifampin can be demonstrated by this procedure.", "PMID": 967671}
{"id": "pubmed23n0032_7101", "title": "Organisation of subunits in chromatin.", "content": "There is considerable current interest in the organisation of nucleosomes in chromatin. A strong X-ray and neutron semi-meridional diffraction peak at approximately 10 nm had previously been attributed to the interparticle specing of a linear array of nucleosomes. This diffraction peak could also result from a close packed helical array of nucleosomes. A direct test of these proposals is whether the 10 nm peak is truly meridional as would be expected for a linear array of nucleosomes or is slightly off the meridian as expected for a helical array. Neutron diffraction studies of H1-depleted chromatin support the latter alternative. The 10 nm peak has maxima which form a cross-pattern with semi-meridional angle of 8 to 9 degrees. This is consistent with a coil of nucleosomes of pitch 10 nm and outer diameter of approximately 30 nm. These dimensions correspond to about six nucleosomes per turn of the coli.", "contents": "Organisation of subunits in chromatin. There is considerable current interest in the organisation of nucleosomes in chromatin. A strong X-ray and neutron semi-meridional diffraction peak at approximately 10 nm had previously been attributed to the interparticle specing of a linear array of nucleosomes. This diffraction peak could also result from a close packed helical array of nucleosomes. A direct test of these proposals is whether the 10 nm peak is truly meridional as would be expected for a linear array of nucleosomes or is slightly off the meridian as expected for a helical array. Neutron diffraction studies of H1-depleted chromatin support the latter alternative. The 10 nm peak has maxima which form a cross-pattern with semi-meridional angle of 8 to 9 degrees. This is consistent with a coil of nucleosomes of pitch 10 nm and outer diameter of approximately 30 nm. These dimensions correspond to about six nucleosomes per turn of the coli.", "PMID": 967672}
{"id": "pubmed23n0032_7102", "title": "Two restriction endonucleases from Bacillus globiggi.", "content": "The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.", "contents": "Two restriction endonucleases from Bacillus globiggi. The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.", "PMID": 967673}
{"id": "pubmed23n0032_7103", "title": "Persistence of the ten-nucleotide repeat in chromatin unfolded in urea, as revealed by digestion with deoxyribonuclease i.", "content": "It is shown by enzymatic digestion of chromatin from rat liver or Guerin ascites tumour (GAT) that treatments, which abolish the 180 base pair repeat, as revealed by digestion with micrococcal nuclease (shearing in salt solutions of medium ionic strength, sonication, fixation with formaldehyde in the presence of 5 M urea), have little effect on the 10 nucleotide repeat, observed in deoxyribonuclease I digests.", "contents": "Persistence of the ten-nucleotide repeat in chromatin unfolded in urea, as revealed by digestion with deoxyribonuclease i. It is shown by enzymatic digestion of chromatin from rat liver or Guerin ascites tumour (GAT) that treatments, which abolish the 180 base pair repeat, as revealed by digestion with micrococcal nuclease (shearing in salt solutions of medium ionic strength, sonication, fixation with formaldehyde in the presence of 5 M urea), have little effect on the 10 nucleotide repeat, observed in deoxyribonuclease I digests.", "PMID": 967674}
{"id": "pubmed23n0032_7104", "title": "An improved synthetic route to the beta-hydroxyethyl esters of 5'-nucleotides.", "content": "A simple method is described for the preparation of the beta-hydroxyethyl esters of nucleoside 5'-phosphates by treatment of the appropriate 2',3'-isopropylidene nucleoside with 2-chloro-2-oxo-1,3-dioxaphospholane. Unambigous structural assignments were based on 13C nmr spectroscopy. Chemical shifts and 13C-31P spin-spin coupling constants are discussed.", "contents": "An improved synthetic route to the beta-hydroxyethyl esters of 5'-nucleotides. A simple method is described for the preparation of the beta-hydroxyethyl esters of nucleoside 5'-phosphates by treatment of the appropriate 2',3'-isopropylidene nucleoside with 2-chloro-2-oxo-1,3-dioxaphospholane. Unambigous structural assignments were based on 13C nmr spectroscopy. Chemical shifts and 13C-31P spin-spin coupling constants are discussed.", "PMID": 967675}
{"id": "pubmed23n0032_7105", "title": "Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.", "content": "Complementary DNA was synthesized using polyadenylated nuclear RNA of cultured Drosophila cells as template. The kinetics of hybridization of this cDNA with nuclear RNA indicated that the complexity of this RNA population is five to ten times greater than that of cytoplasmic mRNA. The same difference in the fraction of DNA represented was obtained when nuclear and cytoplasmic RNA were hybridized with labeled unique sequence DNA. The fraction of the DNA sequences represented in total number of polyadenylated nuclear RNA is much higher than that represented in cytoplasmic RNA.", "contents": "Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells. Complementary DNA was synthesized using polyadenylated nuclear RNA of cultured Drosophila cells as template. The kinetics of hybridization of this cDNA with nuclear RNA indicated that the complexity of this RNA population is five to ten times greater than that of cytoplasmic mRNA. The same difference in the fraction of DNA represented was obtained when nuclear and cytoplasmic RNA were hybridized with labeled unique sequence DNA. The fraction of the DNA sequences represented in total number of polyadenylated nuclear RNA is much higher than that represented in cytoplasmic RNA.", "PMID": 967676}
{"id": "pubmed23n0032_7106", "title": "The base catalysed anomerisation of beta-5-formyluridine; crystal and molecular structure of alpha-5-formyluridine.", "content": "On treatment with strong base beta-5-formyluridine undergoes an anomerisation to give a mixture of the alpha- and beta-anomers. The anomers have been separated by fractional recrystallisation and the absolute configuration of the alpha-anomer has been determined by X-ray analysis.", "contents": "The base catalysed anomerisation of beta-5-formyluridine; crystal and molecular structure of alpha-5-formyluridine. On treatment with strong base beta-5-formyluridine undergoes an anomerisation to give a mixture of the alpha- and beta-anomers. The anomers have been separated by fractional recrystallisation and the absolute configuration of the alpha-anomer has been determined by X-ray analysis.", "PMID": 967677}
{"id": "pubmed23n0032_7107", "title": "Regenerating rat liver DNA polymerases: disimilitude or relationship between nuclear and cytoplasmic enzymes?", "content": "The possible relationship between the nuclear and cytoplasmic DNA polymerases of regenerating rat liver was studied by sucrose gradient analysis, salt dissociation, and with specific inhibitors. After aqueous subcellular fractionation and removal of the nuclear membranes, three species of DNA-dependent DNA polymerases were characterized: 1) a DNA polymerase-beta in the nuclei. 2) a DNA polymerase-alpha in the cytosol which was not dissociated at high salt concentrations; and 3) an intermediate form in the cytosol and in the Triton wash containing the nuclear membranes. The latter form behaved like DNA polymerase-alpha et low salt concentration but was dissociated at high salt concentrations to a low molecular weight species with properties like DNA polymerase-beta (resistance to inhibition by N-ethylmaleimide, heparin and KCL). In vitro reassociation experiments suggest that this intermediate form corresponds to the association of DNA polymerase-beta with a membrane component or cytoplasmic protein(s) which appear(s) in regenerating rat liver.", "contents": "Regenerating rat liver DNA polymerases: disimilitude or relationship between nuclear and cytoplasmic enzymes? The possible relationship between the nuclear and cytoplasmic DNA polymerases of regenerating rat liver was studied by sucrose gradient analysis, salt dissociation, and with specific inhibitors. After aqueous subcellular fractionation and removal of the nuclear membranes, three species of DNA-dependent DNA polymerases were characterized: 1) a DNA polymerase-beta in the nuclei. 2) a DNA polymerase-alpha in the cytosol which was not dissociated at high salt concentrations; and 3) an intermediate form in the cytosol and in the Triton wash containing the nuclear membranes. The latter form behaved like DNA polymerase-alpha et low salt concentration but was dissociated at high salt concentrations to a low molecular weight species with properties like DNA polymerase-beta (resistance to inhibition by N-ethylmaleimide, heparin and KCL). In vitro reassociation experiments suggest that this intermediate form corresponds to the association of DNA polymerase-beta with a membrane component or cytoplasmic protein(s) which appear(s) in regenerating rat liver.", "PMID": 967678}
{"id": "pubmed23n0032_7108", "title": "Chromatin and nucleosome structure.", "content": "Chromatin nucleosomes (mononucleosomes through pentanucleosomes) have been isolated by staphylococcal nuclease digestion of calf thymus nuclei. The peak value ellipticity is the same for all oligomers, 1900 deg cm2, mol-1 at 280-nm, 23 degrees C. The dh280/dT vs T show a progressive increase in Tm of the main thermal band (73.5 degrees C, monomer; 79 degrees C, pentamer). Very small amounts of free DNA can be observed in the melting profiles, and shoulders at 60 degrees C and 93 degrees C appear and increase in magnitude as the particle size increases. The magnitude of the change, delta[theta]280, increases with oligomer size. This pattern could result from an initial unfolding of an asymmetric assembly of nucleosomes (polynucleosome superhelix) in addition to the denaturation of the internal nucleosome structure, and a subsequent or simultaneous denaturation of the double strand DNA. The extent of this unfolding appears to depend upon the size of the oligomer and therefore implies interactions between asymmetrically assembled neighboring nucleosomes.", "contents": "Chromatin and nucleosome structure. Chromatin nucleosomes (mononucleosomes through pentanucleosomes) have been isolated by staphylococcal nuclease digestion of calf thymus nuclei. The peak value ellipticity is the same for all oligomers, 1900 deg cm2, mol-1 at 280-nm, 23 degrees C. The dh280/dT vs T show a progressive increase in Tm of the main thermal band (73.5 degrees C, monomer; 79 degrees C, pentamer). Very small amounts of free DNA can be observed in the melting profiles, and shoulders at 60 degrees C and 93 degrees C appear and increase in magnitude as the particle size increases. The magnitude of the change, delta[theta]280, increases with oligomer size. This pattern could result from an initial unfolding of an asymmetric assembly of nucleosomes (polynucleosome superhelix) in addition to the denaturation of the internal nucleosome structure, and a subsequent or simultaneous denaturation of the double strand DNA. The extent of this unfolding appears to depend upon the size of the oligomer and therefore implies interactions between asymmetrically assembled neighboring nucleosomes.", "PMID": 967679}
{"id": "pubmed23n0032_7109", "title": "Circular dichroism studies of ethidium bromide binding to the isolated nucleolus.", "content": "Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of the induced ellipticity comes from the DNA component. In nucleoli with an RNA/DNA = 0.8/1.0 the RNA component gives only 20% of the total ellipticity when measured at an ethidium bromide/DNA = 0.25. Spectro-fluorometric titration shows that ethidium bromide intercalates mostly into DNA in nucleoli. Both circular dichroism and fluorescence studies indicate that both DNA and RNA components in isolated nucleoli are less accessible to intercalating binding by ethidium bromide when compared to purified nucleolar DNA, DNA in chromatin or purified ribosomal RNA. Circular dichroic measurements of intercalating binding of ethidium bromide to to nucleoli may be used to study changes in nucleoli under different physiological or pathological conditions.", "contents": "Circular dichroism studies of ethidium bromide binding to the isolated nucleolus. Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of the induced ellipticity comes from the DNA component. In nucleoli with an RNA/DNA = 0.8/1.0 the RNA component gives only 20% of the total ellipticity when measured at an ethidium bromide/DNA = 0.25. Spectro-fluorometric titration shows that ethidium bromide intercalates mostly into DNA in nucleoli. Both circular dichroism and fluorescence studies indicate that both DNA and RNA components in isolated nucleoli are less accessible to intercalating binding by ethidium bromide when compared to purified nucleolar DNA, DNA in chromatin or purified ribosomal RNA. Circular dichroic measurements of intercalating binding of ethidium bromide to to nucleoli may be used to study changes in nucleoli under different physiological or pathological conditions.", "PMID": 967680}
{"id": "pubmed23n0032_7110", "title": "Thermal denaturation of sheared and unsheared chromatin by absorption and circular dichroism measurements.", "content": "Thermal denaturation of chromatin is observed by simultaneously monitoring absorption and circular dichroism at 276 nm as functions of temperature. Either observation indicates that sheared chromatins shows less thermal stability than native chromatin. The temperature-dependent ellipticities at 276 nm of these chromatins show features not seen in the absorption curves: the ellipticity of unsheared chromatin increases with temperature, while this increase is abolished or greatly reduced in the same chromatin after shearing. After its first thermal transition (prior to the helix-coli transition) the unsheared chromatin achieves the same ellipticity as sheared chromatin.", "contents": "Thermal denaturation of sheared and unsheared chromatin by absorption and circular dichroism measurements. Thermal denaturation of chromatin is observed by simultaneously monitoring absorption and circular dichroism at 276 nm as functions of temperature. Either observation indicates that sheared chromatins shows less thermal stability than native chromatin. The temperature-dependent ellipticities at 276 nm of these chromatins show features not seen in the absorption curves: the ellipticity of unsheared chromatin increases with temperature, while this increase is abolished or greatly reduced in the same chromatin after shearing. After its first thermal transition (prior to the helix-coli transition) the unsheared chromatin achieves the same ellipticity as sheared chromatin.", "PMID": 967681}
{"id": "pubmed23n0032_7111", "title": "Preparation and properties of a poly A resin and its use in the isolation of naturally occurring poly U sequences.", "content": "A method for the covalent attachment of poly A, as well as other nucleic acids and nucleosides, to a methylene dianiline derivative of starch is described. The properties of this poly A resin and its use for the recovery of poly U sequences from both nuclear and cytoplasmic extracts of HeLa cells is described.", "contents": "Preparation and properties of a poly A resin and its use in the isolation of naturally occurring poly U sequences. A method for the covalent attachment of poly A, as well as other nucleic acids and nucleosides, to a methylene dianiline derivative of starch is described. The properties of this poly A resin and its use for the recovery of poly U sequences from both nuclear and cytoplasmic extracts of HeLa cells is described.", "PMID": 967682}
{"id": "pubmed23n0032_7112", "title": "Structural studies on the two forms of 8-bromo-2',3'-O-isopropylideneadenosine.", "content": "The crystal and molecular structures of two forms of 8-bromo-2',3'-O-isopropylideneadenosine have been determined by X-ray methods. In one form, the molecular structure has planar conformation in the sugar moiety and no intramolecular hydrogen bond. On the other hand, the molecular structure of the second form has C(2')-endo conformation and an intramolecular hydrogen bond. No stacking interaction between adjacent bases is found in either form, but two modes of the base-pairing hydrogen bond exist in the second form.", "contents": "Structural studies on the two forms of 8-bromo-2',3'-O-isopropylideneadenosine. The crystal and molecular structures of two forms of 8-bromo-2',3'-O-isopropylideneadenosine have been determined by X-ray methods. In one form, the molecular structure has planar conformation in the sugar moiety and no intramolecular hydrogen bond. On the other hand, the molecular structure of the second form has C(2')-endo conformation and an intramolecular hydrogen bond. No stacking interaction between adjacent bases is found in either form, but two modes of the base-pairing hydrogen bond exist in the second form.", "PMID": 967683}
{"id": "pubmed23n0032_7113", "title": "The conservation of poly-A-containing RNA during the dormant state of the moss Polytrichum commune.", "content": "The moss Polytrichum commune can be dried to less than 2% of free water and kept for some weeks without losing its viability. Upon rehydration of the moss, protein synthesis starts about 60 minutes before incorporation of radioactive precursors into RNA can be observed. Pulse labeled total RNA and poly-A-containing RNA do not show quantitative or qualitative alterations during desiccation up to 20 days.", "contents": "The conservation of poly-A-containing RNA during the dormant state of the moss Polytrichum commune. The moss Polytrichum commune can be dried to less than 2% of free water and kept for some weeks without losing its viability. Upon rehydration of the moss, protein synthesis starts about 60 minutes before incorporation of radioactive precursors into RNA can be observed. Pulse labeled total RNA and poly-A-containing RNA do not show quantitative or qualitative alterations during desiccation up to 20 days.", "PMID": 967684}
{"id": "pubmed23n0032_7114", "title": "Chicken globin gene number.", "content": "Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DNA, and with protein data.", "contents": "Chicken globin gene number. Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DNA, and with protein data.", "PMID": 967685}
{"id": "pubmed23n0032_7115", "title": "Sequence specific fragments in the field desorption mass spectra of dinucleoside phosphates.", "content": "In field desorption mass spectrometry of ribodinucleoside phosphates the formation of nucleoside cyclophosphates can be used to determine the base sequence.", "contents": "Sequence specific fragments in the field desorption mass spectra of dinucleoside phosphates. In field desorption mass spectrometry of ribodinucleoside phosphates the formation of nucleoside cyclophosphates can be used to determine the base sequence.", "PMID": 967686}
{"id": "pubmed23n0032_7116", "title": "Effects of phytohormones on thermal denaturation profiles of Cymbidium DNA: indication of differential DNA replication.", "content": "Protocorm pieces of the orchid Cymbidium were aseptically cultured either without phytohormones, or with one of the growth promoting substances, auxin cytokinin, and gibberellin. The derivative melting profiles of the extracted DNA's differ from each other with respect to the size of various AT- and GC- rich fractions. Evidence has been obtained for the increase of the more AT--rich fractions in auxin-treated cultures, while gibberellin stimulated the expansion of the GC-rich fractions. These results are consistent with earlier cytological and cytochemical findings and indicate the involvement of hormone-controlled differential DNA replication in the development of Cymbidium protocorms in vitro.", "contents": "Effects of phytohormones on thermal denaturation profiles of Cymbidium DNA: indication of differential DNA replication. Protocorm pieces of the orchid Cymbidium were aseptically cultured either without phytohormones, or with one of the growth promoting substances, auxin cytokinin, and gibberellin. The derivative melting profiles of the extracted DNA's differ from each other with respect to the size of various AT- and GC- rich fractions. Evidence has been obtained for the increase of the more AT--rich fractions in auxin-treated cultures, while gibberellin stimulated the expansion of the GC-rich fractions. These results are consistent with earlier cytological and cytochemical findings and indicate the involvement of hormone-controlled differential DNA replication in the development of Cymbidium protocorms in vitro.", "PMID": 967687}
{"id": "pubmed23n0032_7117", "title": "The absence of histone H1 from the chromatin fraction obtained by sonication of calf thymus nuclei under \"quasiphysiological\" ionic conditions.", "content": "The minor chromatin fraction was isolated from the sonicated calf thymus nuclei on the basis of its differential solubility in the \"quasiphysiological\" salt medium (0.1 M KCl-0.05 M NaCl-l mM MgCl2-1 mM CaCl2). Histone Hl is almost completely absent from this fraction. DNA isolated from this fraction occurs in three discrete low mol. wt. fragments. The fraction of chromatin which lacks histone Hl can also be obtained by two other methods. On of them consists in salt precipitation of the chromatin gel and its subsequent sonication. The second method includes precipitation of the sonicated chromatin gel by salts. In the first case the properties of the chromatin fraction which remains in the supernatant after centrifugation closely resemble those of the original salt-soluble nuclear fraction. The second method yields supernatant fraction also lacking histone Hl but containing heterogeneous DNA. Comparisons were also made of the sonically-solubilized nuclear fractions obtained in the complete salt medium and its mono and divalent cationic constituents.", "contents": "The absence of histone H1 from the chromatin fraction obtained by sonication of calf thymus nuclei under \"quasiphysiological\" ionic conditions. The minor chromatin fraction was isolated from the sonicated calf thymus nuclei on the basis of its differential solubility in the \"quasiphysiological\" salt medium (0.1 M KCl-0.05 M NaCl-l mM MgCl2-1 mM CaCl2). Histone Hl is almost completely absent from this fraction. DNA isolated from this fraction occurs in three discrete low mol. wt. fragments. The fraction of chromatin which lacks histone Hl can also be obtained by two other methods. On of them consists in salt precipitation of the chromatin gel and its subsequent sonication. The second method includes precipitation of the sonicated chromatin gel by salts. In the first case the properties of the chromatin fraction which remains in the supernatant after centrifugation closely resemble those of the original salt-soluble nuclear fraction. The second method yields supernatant fraction also lacking histone Hl but containing heterogeneous DNA. Comparisons were also made of the sonically-solubilized nuclear fractions obtained in the complete salt medium and its mono and divalent cationic constituents.", "PMID": 967688}
{"id": "pubmed23n0032_7118", "title": "Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells.", "content": "In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE-and CM-cellulose chromatography and resulted in an RNAas D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly (rC). Significant levels of RNAse H activity against poly (rA)-poly (dT) were still present in these preparations.", "contents": "Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells. In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE-and CM-cellulose chromatography and resulted in an RNAas D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly (rC). Significant levels of RNAse H activity against poly (rA)-poly (dT) were still present in these preparations.", "PMID": 967689}
{"id": "pubmed23n0032_7119", "title": "On the mechanism of tRNA methylase-tRNA recognition.", "content": "In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneously present in the reaction mixture. The inhibitory action of yeast tRNASer, possessing m5c in position 29, on the methylation of C29 in other individual tRNAs was shown. Yeast tRNAVal which possesses an A residue in position 27 was shown to inhibit the methylation of G27 in E. coli tRNAMet. The data obtained confirm the suggestion that tRNA methylases recognizes the tertiary structure of tRNAs. They show also that the recognition and the proper catalytic action are two  autonomous processes and that the former at least in its first stage is rather unspecific.", "contents": "On the mechanism of tRNA methylase-tRNA recognition. In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneously present in the reaction mixture. The inhibitory action of yeast tRNASer, possessing m5c in position 29, on the methylation of C29 in other individual tRNAs was shown. Yeast tRNAVal which possesses an A residue in position 27 was shown to inhibit the methylation of G27 in E. coli tRNAMet. The data obtained confirm the suggestion that tRNA methylases recognizes the tertiary structure of tRNAs. They show also that the recognition and the proper catalytic action are two  autonomous processes and that the former at least in its first stage is rather unspecific.", "PMID": 967690}
{"id": "pubmed23n0032_7120", "title": "Metabolism of pyrimidine L-nucleosides.", "content": "The intraperitoneal application of L-nucleosides (L-Cyd, L-Urd, L-dThd) to mice results in distribution of these compounds into tissues of the organism and their gradual excretion in the unchanged form. The residual level has been observed with L-ribonucleosides only and contains in addition to the L-nucleoside its 5'-phosphate. The phosphorylation in vivo is catalyzed by nucleoside-kinase and utilizes ATP as the phosphate donor while glycerol 1-phosphate and creatine phosphate are inactive. The L-cytidine derivatives are in vivo deaminated to the derivatives of L-uridine. On the other hand, when L-uridine is applied in vivo, derivatives of L-cytidine are obtained on the level of both the nucleoside and 5'-ribonucleotide.", "contents": "Metabolism of pyrimidine L-nucleosides. The intraperitoneal application of L-nucleosides (L-Cyd, L-Urd, L-dThd) to mice results in distribution of these compounds into tissues of the organism and their gradual excretion in the unchanged form. The residual level has been observed with L-ribonucleosides only and contains in addition to the L-nucleoside its 5'-phosphate. The phosphorylation in vivo is catalyzed by nucleoside-kinase and utilizes ATP as the phosphate donor while glycerol 1-phosphate and creatine phosphate are inactive. The L-cytidine derivatives are in vivo deaminated to the derivatives of L-uridine. On the other hand, when L-uridine is applied in vivo, derivatives of L-cytidine are obtained on the level of both the nucleoside and 5'-ribonucleotide.", "PMID": 967691}
{"id": "pubmed23n0032_7121", "title": "Effects of DL-ethionine on mouse liver tRNA base composition.", "content": "Treatment of mice with DL-ethionine and adenine causes a reduction of all methylated bases of liver tRNA. This effect is dose-dependent and specific for the methylated bases. Individual methylated components are affected to different extents, m22G being most sensitive to inhibition.", "contents": "Effects of DL-ethionine on mouse liver tRNA base composition. Treatment of mice with DL-ethionine and adenine causes a reduction of all methylated bases of liver tRNA. This effect is dose-dependent and specific for the methylated bases. Individual methylated components are affected to different extents, m22G being most sensitive to inhibition.", "PMID": 967692}
{"id": "pubmed23n0032_7122", "title": "Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.", "content": "We describe a method of isolating a homogeneous population of \"trimmed\" monomeric nucleosomes from Hela cells. These nucleoprotein particles contain a 140 +/- 5 base pair length of DNA and have a histone/DNA ratio of 1.2. They lack H1 and contain equal amounts of the four smaller histones. The DNA contains no single strand nicks. The particles sediment with an S20,w of 11S in D2O density gradients. After formaldehyde fixation, they band at a density of 1.4370 in neutral CsCl. Digestion of nucleosomes with either micrococcal nuclease or DNase I generates the same pattern of DNA fragments observed when intact nuclei are digested. Circular dichroism spectra indicate that the 280 nm positive ellipticity maximum of nucleosomes is about one-half that of chromatin. In the presence of 6 M urea, nucleosomes sediment with an S20,w of 6S, have a multiphasic thermal denaturation profile, and exhibit a circular dichroic spectrum nearly identical to that of B-form DNA. Our yield of purified nucleosomes (10-15% of the input DNA) is similar to the yields of other methods; our nucleosome population is substantially more homogeneous than those previously reported.", "contents": "Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells. We describe a method of isolating a homogeneous population of \"trimmed\" monomeric nucleosomes from Hela cells. These nucleoprotein particles contain a 140 +/- 5 base pair length of DNA and have a histone/DNA ratio of 1.2. They lack H1 and contain equal amounts of the four smaller histones. The DNA contains no single strand nicks. The particles sediment with an S20,w of 11S in D2O density gradients. After formaldehyde fixation, they band at a density of 1.4370 in neutral CsCl. Digestion of nucleosomes with either micrococcal nuclease or DNase I generates the same pattern of DNA fragments observed when intact nuclei are digested. Circular dichroism spectra indicate that the 280 nm positive ellipticity maximum of nucleosomes is about one-half that of chromatin. In the presence of 6 M urea, nucleosomes sediment with an S20,w of 6S, have a multiphasic thermal denaturation profile, and exhibit a circular dichroic spectrum nearly identical to that of B-form DNA. Our yield of purified nucleosomes (10-15% of the input DNA) is similar to the yields of other methods; our nucleosome population is substantially more homogeneous than those previously reported.", "PMID": 967693}
{"id": "pubmed23n0032_7123", "title": "Protection of particular cleavage sites of restriction endonucleases by distamycin A and actinomycin D.", "content": "It is shown here that distamycin A and actinomycin D can protect the recognition sites of endo R.EcoRI, EcoRII, HindII, HindIII, HpaI and HpaII from the attack of these restriction endonucleases. At proper distamycin concentrations only two endo R.EcoRI sites of phage lambda DNA are available for the restriction enzyme--sRI1 and sRI4. This phenomenon results in the appearance of larger DNA fragments comprising several consecutive fragments of endo R.EcoRI complete cleavage. The distamycin fragments isolated from the agarose gels can be subsequently cleaved by endo R.EcoRI with the yield of the fragments of complete digestion. We have compared the effect of distamycin A and actinomycin D on a number of restriction endonucleases having different nucleotide sequences in the recognition sites and established that antibiotic action depends on the nucleotide sequences of the recognition sites and their closest environment", "contents": "Protection of particular cleavage sites of restriction endonucleases by distamycin A and actinomycin D. It is shown here that distamycin A and actinomycin D can protect the recognition sites of endo R.EcoRI, EcoRII, HindII, HindIII, HpaI and HpaII from the attack of these restriction endonucleases. At proper distamycin concentrations only two endo R.EcoRI sites of phage lambda DNA are available for the restriction enzyme--sRI1 and sRI4. This phenomenon results in the appearance of larger DNA fragments comprising several consecutive fragments of endo R.EcoRI complete cleavage. The distamycin fragments isolated from the agarose gels can be subsequently cleaved by endo R.EcoRI with the yield of the fragments of complete digestion. We have compared the effect of distamycin A and actinomycin D on a number of restriction endonucleases having different nucleotide sequences in the recognition sites and established that antibiotic action depends on the nucleotide sequences of the recognition sites and their closest environment", "PMID": 967694}
{"id": "pubmed23n0032_7124", "title": "Effect of ribosylzeatin isomers on the enzymatic degradation of N6-(delta2-isopentenyl) adenosine.", "content": "Cytokinin oxidase has been partially purified from cultured tobacco tissue. This enzyme converts N6-(delta2-isopentenyl)-adenosine to adenosine. The reaction is inhibited by the two isomers of ribosylzeatin [n6-4-hydroxy-3-methylbut-2-enyl)adenosine]. Trans-ribosylzeatin inhibits the reaction more than the cis-isomer.", "contents": "Effect of ribosylzeatin isomers on the enzymatic degradation of N6-(delta2-isopentenyl) adenosine. Cytokinin oxidase has been partially purified from cultured tobacco tissue. This enzyme converts N6-(delta2-isopentenyl)-adenosine to adenosine. The reaction is inhibited by the two isomers of ribosylzeatin [n6-4-hydroxy-3-methylbut-2-enyl)adenosine]. Trans-ribosylzeatin inhibits the reaction more than the cis-isomer.", "PMID": 967695}
{"id": "pubmed23n0032_7125", "title": "Some properties of tobacco protoplast chromatin.", "content": "Chromatin was prepared from tobacco-leaf protoplasts. Its solubility in increasing molarities of NaCl was studied and the structure of the soluble fraction observed by electron microscopy. We demonstrate that in plants, the DNA and histones are associated in beaded structures similar to those called omicron-bodies or nucleosomes in animal chromatin. The nucleosomes were associated with DNA in either compact or extended forms. The compact arrangement was predominant in the fraction solubilized between 0.1 and 0.4 M NaCl. The extended form, present at 0.5 and 0.6 M NaCl. showed DNA filaments of various lengths interspacing the nucleosomes. At these ionic strengths ring structures were present, associated with the DNA. At 0.7 M NaCl and above, only DNA filaments were present, occasionally associated with big rings, and nucleosomes were compoetely dissociated. Free DNA molecules were present at all ionic strengths used. The possible origin and significance of the rings are discussed.", "contents": "Some properties of tobacco protoplast chromatin. Chromatin was prepared from tobacco-leaf protoplasts. Its solubility in increasing molarities of NaCl was studied and the structure of the soluble fraction observed by electron microscopy. We demonstrate that in plants, the DNA and histones are associated in beaded structures similar to those called omicron-bodies or nucleosomes in animal chromatin. The nucleosomes were associated with DNA in either compact or extended forms. The compact arrangement was predominant in the fraction solubilized between 0.1 and 0.4 M NaCl. The extended form, present at 0.5 and 0.6 M NaCl. showed DNA filaments of various lengths interspacing the nucleosomes. At these ionic strengths ring structures were present, associated with the DNA. At 0.7 M NaCl and above, only DNA filaments were present, occasionally associated with big rings, and nucleosomes were compoetely dissociated. Free DNA molecules were present at all ionic strengths used. The possible origin and significance of the rings are discussed.", "PMID": 967696}
{"id": "pubmed23n0032_7126", "title": "Electrocardiographic surface mapping of the heart following myocardial infarction and the influence of beta-blockade.", "content": "It is generally accepted that ST segment elevation is one of the characteristic electrocardiographic features of acute myocardial infarction. In experimental myocardial infarction the degree of ST segment elevation has been related to the degree of change in coronary flow. Surface mapping in patients of the electrocardiographic potentials gives an indirect representation of the epicardial ST segment change and in indication of the area of underlying myocardial damage. A number of patients who had sustained myocardial infar-tion within 72 hours were studied. All the patients had a history of prolonged ischaemic cardiac pain, electrocardiographic changes and a rise in serum enzymes. Electrocardiagrams were recorded from 72 points on the chest surface. Control maps were constructed from the ST segment changes measured at each of these points and plotted on a standard diagram. Electrocardiograms were recorded during a control period immediately before 20 mg of practolol was given intravenously over a period of five minutes. In all patients practolol produced a significant reduction in the area of ST segment elevation as represented by a reduction in the number of points with ST elevation of more than 2 mm. The significance of these findings is discussed.", "contents": "Electrocardiographic surface mapping of the heart following myocardial infarction and the influence of beta-blockade. It is generally accepted that ST segment elevation is one of the characteristic electrocardiographic features of acute myocardial infarction. In experimental myocardial infarction the degree of ST segment elevation has been related to the degree of change in coronary flow. Surface mapping in patients of the electrocardiographic potentials gives an indirect representation of the epicardial ST segment change and in indication of the area of underlying myocardial damage. A number of patients who had sustained myocardial infar-tion within 72 hours were studied. All the patients had a history of prolonged ischaemic cardiac pain, electrocardiographic changes and a rise in serum enzymes. Electrocardiagrams were recorded from 72 points on the chest surface. Control maps were constructed from the ST segment changes measured at each of these points and plotted on a standard diagram. Electrocardiograms were recorded during a control period immediately before 20 mg of practolol was given intravenously over a period of five minutes. In all patients practolol produced a significant reduction in the area of ST segment elevation as represented by a reduction in the number of points with ST elevation of more than 2 mm. The significance of these findings is discussed.", "PMID": 967768}
{"id": "pubmed23n0032_7127", "title": "Propranolol in acute myocardial infarction in man: effects of haemodynamics and myocardial oxygenation.", "content": "Improvement of myocardial oxygenation is a major goal in the treatment of ischaemic heart disease. Propranolol, 0-1 mg/kg intravenously, was administered to 20 patients with acute myocardial infarction without clinical evidence of left ventricular failure. The most important haemodynamic response was a substantial decrease in myocardial contractility. This was reflected by a fall in cardiac index (average of 0-4 l/min/M2, P less than 0-001) and of arterial mean pressure (average of 16 mmHg, P less than 0-001) with little change in systemic vascular resistance. Heart rate, not strikingly increased at the control state in the majority of patients, decreased an average of 7 beats/minute (P less than 0-001). Pulmonary wedge pressure varied; it decreased in 6 patients with high values (greater than 15 mmHg) prior to propranolol. These changes in wedge pressure in the presence of decreased contractility are probably related to improved left ventricular compliance, produced by propranolol. Propranolol markedly improved myocardial metabolism. Arterial-coronary sinus oxygen difference decreased an average of 0-72 ml/100 ml (P less than 0-001). Myocardial lactate production shifted to extraction (average of -8% to 14%) or the rate of lactate extraction increased (average of 20% to 29%). Coronary blood flow decreased an average of 13 ml/100 g/min (P less than 0-001). The finding, that myocardial metabolism improved, suggests that decrease in coronary blood flow was mediated by reduced myocardial oxygen requirements. None of the 20 patients developed left ventricular failure or other complications related to beta-adrenergic blockade. Severe chest pain, unresponsive to conventional therapy in four patients, was relieved by propranolol. These findings demonstrate that acutely administered propranolol improves myocardila oxygenation in patients with uncomplicated acute infarction without endangering perfusion of other vital organs.", "contents": "Propranolol in acute myocardial infarction in man: effects of haemodynamics and myocardial oxygenation. Improvement of myocardial oxygenation is a major goal in the treatment of ischaemic heart disease. Propranolol, 0-1 mg/kg intravenously, was administered to 20 patients with acute myocardial infarction without clinical evidence of left ventricular failure. The most important haemodynamic response was a substantial decrease in myocardial contractility. This was reflected by a fall in cardiac index (average of 0-4 l/min/M2, P less than 0-001) and of arterial mean pressure (average of 16 mmHg, P less than 0-001) with little change in systemic vascular resistance. Heart rate, not strikingly increased at the control state in the majority of patients, decreased an average of 7 beats/minute (P less than 0-001). Pulmonary wedge pressure varied; it decreased in 6 patients with high values (greater than 15 mmHg) prior to propranolol. These changes in wedge pressure in the presence of decreased contractility are probably related to improved left ventricular compliance, produced by propranolol. Propranolol markedly improved myocardial metabolism. Arterial-coronary sinus oxygen difference decreased an average of 0-72 ml/100 ml (P less than 0-001). Myocardial lactate production shifted to extraction (average of -8% to 14%) or the rate of lactate extraction increased (average of 20% to 29%). Coronary blood flow decreased an average of 13 ml/100 g/min (P less than 0-001). The finding, that myocardial metabolism improved, suggests that decrease in coronary blood flow was mediated by reduced myocardial oxygen requirements. None of the 20 patients developed left ventricular failure or other complications related to beta-adrenergic blockade. Severe chest pain, unresponsive to conventional therapy in four patients, was relieved by propranolol. These findings demonstrate that acutely administered propranolol improves myocardila oxygenation in patients with uncomplicated acute infarction without endangering perfusion of other vital organs.", "PMID": 967769}
{"id": "pubmed23n0032_7128", "title": "Propranolol in patients with acute myocardial infarction at the Royal Melbourne Hospital from 1965: a review.", "content": "This review presents our experience in the use of propranolol in the management of cardiac infarction. The drug has now been shown to have a definite place, not only in  the prevention of cardiac infarction, but also in the treatment of some of the complications. The drug has also been advocated because it may minimize the extent of myocardial damage.", "contents": "Propranolol in patients with acute myocardial infarction at the Royal Melbourne Hospital from 1965: a review. This review presents our experience in the use of propranolol in the management of cardiac infarction. The drug has now been shown to have a definite place, not only in  the prevention of cardiac infarction, but also in the treatment of some of the complications. The drug has also been advocated because it may minimize the extent of myocardial damage.", "PMID": 967770}
{"id": "pubmed23n0032_7129", "title": "The use of drug concentration measurements in studies of the therapeutic response to propranolol.", "content": "The dose of propranolol which produces the optimal therapeutic effect in patients with angina pectoris has been found to vary widely among patients. Because the plasma concentration of propranolol also differs markedly due to interpatient variability in absorption it seemed possible that this might be the reason for the wide range of effective doses in angina and that plasma propranolol might provide a useful guide to therapeutic response. To examine this possibility we selected ten patients with coronary artery disease complicated by angina and studied them at varying propranolol doses to a maximum of 320 mg/day. Exercise capacity was tested on a treadmill and plasma propranolol concentration was measured by gas liquid chromatography. In seven normal subjects beta-blockade was quantified precisely as inhibition of exercise tachycardia and was related to plasma propranolol levels at various doses. Maximal beta-blockade occurred at 100 ng/ml of plasma propranolol, but the dose response curve of blockade was relatively flat and the ED50 of plasma propranolol was 8+/-1 ng/ml. In the patients maximal therapeutic benefit from propranolol occurred at 30+/-7 ng/ml and at a dose of 144 mg/day. This resulted in an increase in exercise capacity from an estimated 12-7+/-0-8 ml/kg/min of oxygen consumption during control to 17-2+/-1-1 ml/kg/min on the drug. Thus, there was a wide variation of both dose and concentration among these patients at the maximum therapeutic response. However, when plasma propranolol was related to pharmacologic activity, the maximum therapeutic response was observed between 64 and 98% of total blockade. These studies indicated the extent of beta-blockade necessary to produce an effective therapeutic response in angina, but demonstrate that plasma drug levels provide no practical guide to therapy in patients with angina pectoris. A further study was conducted measuring plasma propranolol in twenty hypertensive patients to investigate the fall in blood pressure in relations to the change in plasma renin activity and the inhibition of cardiac adrenergic receptors. The inhibition of plasma renin closely resemble the response seen in heart rate inhibition in that the maximum response is seen at 100 ng/ml and the ED50 was 11 ng/ml. In contrast propranolol is shown only to begin to have a significant effect on blood pressure at a plasma level of 30 ng/ml and the effect becomes progressively greater as the plasma level increases. This suggests that the hypotensive effect of propranolol may be dissociated from the beta-blocking effects of cardiac and renin releasing receptors.", "contents": "The use of drug concentration measurements in studies of the therapeutic response to propranolol. The dose of propranolol which produces the optimal therapeutic effect in patients with angina pectoris has been found to vary widely among patients. Because the plasma concentration of propranolol also differs markedly due to interpatient variability in absorption it seemed possible that this might be the reason for the wide range of effective doses in angina and that plasma propranolol might provide a useful guide to therapeutic response. To examine this possibility we selected ten patients with coronary artery disease complicated by angina and studied them at varying propranolol doses to a maximum of 320 mg/day. Exercise capacity was tested on a treadmill and plasma propranolol concentration was measured by gas liquid chromatography. In seven normal subjects beta-blockade was quantified precisely as inhibition of exercise tachycardia and was related to plasma propranolol levels at various doses. Maximal beta-blockade occurred at 100 ng/ml of plasma propranolol, but the dose response curve of blockade was relatively flat and the ED50 of plasma propranolol was 8+/-1 ng/ml. In the patients maximal therapeutic benefit from propranolol occurred at 30+/-7 ng/ml and at a dose of 144 mg/day. This resulted in an increase in exercise capacity from an estimated 12-7+/-0-8 ml/kg/min of oxygen consumption during control to 17-2+/-1-1 ml/kg/min on the drug. Thus, there was a wide variation of both dose and concentration among these patients at the maximum therapeutic response. However, when plasma propranolol was related to pharmacologic activity, the maximum therapeutic response was observed between 64 and 98% of total blockade. These studies indicated the extent of beta-blockade necessary to produce an effective therapeutic response in angina, but demonstrate that plasma drug levels provide no practical guide to therapy in patients with angina pectoris. A further study was conducted measuring plasma propranolol in twenty hypertensive patients to investigate the fall in blood pressure in relations to the change in plasma renin activity and the inhibition of cardiac adrenergic receptors. The inhibition of plasma renin closely resemble the response seen in heart rate inhibition in that the maximum response is seen at 100 ng/ml and the ED50 was 11 ng/ml. In contrast propranolol is shown only to begin to have a significant effect on blood pressure at a plasma level of 30 ng/ml and the effect becomes progressively greater as the plasma level increases. This suggests that the hypotensive effect of propranolol may be dissociated from the beta-blocking effects of cardiac and renin releasing receptors.", "PMID": 967771}
{"id": "pubmed23n0032_7130", "title": "Haemodynamic effects of beta-blockade in patients with angina pectoris.", "content": "The pressure-volume relationship of the intact human ventricle during ischaemia and following the administration of a drug commonly used to treat ischaemic heart disease has been studied. Precise quantitation of the effects of the non-selective beta-adrenergic blocking drug propranolol (0-15 mg/kg body weight) on left ventricular function, segmental wall motion and diastolic pressure-volume relationship in man has been performed. High fidelity left ventricular pressure measurements and simultaneous single-plane angiograms were recorded and volumes calculated by a light-pen computer system. Patients were transvenously atrially paced for constancy of heart rate during drug infusion or paced to angina. In patients with coronary heart disease paced to angina, logarithmic pressure-volume relationship showed change in slope (P greater than 0-05) of the regression line and upward shift in intercept b (+0-25; P greater than 0-001). The diastolic effects of propranolol may include substantial increases in ventricular volumes in those patients with impaired cardiac function. With respect to the intact human ventricle, propranolol may increase diastolic volume for a given level of ventricular pressure. Thus, in a static sense, the ventricle in these patients could be viewed as being more compliant following propranolol administration. However, the fact that the length-tension relationship as measured by the slope of the logarithmic pressure versus volume plot is unaltered by propranolol, suggests that the muscle comprising the ventricle itself exhibits no alteration in its passive elastic properties.", "contents": "Haemodynamic effects of beta-blockade in patients with angina pectoris. The pressure-volume relationship of the intact human ventricle during ischaemia and following the administration of a drug commonly used to treat ischaemic heart disease has been studied. Precise quantitation of the effects of the non-selective beta-adrenergic blocking drug propranolol (0-15 mg/kg body weight) on left ventricular function, segmental wall motion and diastolic pressure-volume relationship in man has been performed. High fidelity left ventricular pressure measurements and simultaneous single-plane angiograms were recorded and volumes calculated by a light-pen computer system. Patients were transvenously atrially paced for constancy of heart rate during drug infusion or paced to angina. In patients with coronary heart disease paced to angina, logarithmic pressure-volume relationship showed change in slope (P greater than 0-05) of the regression line and upward shift in intercept b (+0-25; P greater than 0-001). The diastolic effects of propranolol may include substantial increases in ventricular volumes in those patients with impaired cardiac function. With respect to the intact human ventricle, propranolol may increase diastolic volume for a given level of ventricular pressure. Thus, in a static sense, the ventricle in these patients could be viewed as being more compliant following propranolol administration. However, the fact that the length-tension relationship as measured by the slope of the logarithmic pressure versus volume plot is unaltered by propranolol, suggests that the muscle comprising the ventricle itself exhibits no alteration in its passive elastic properties.", "PMID": 967772}
{"id": "pubmed23n0032_7131", "title": "Patient acceptability of propranolol and the occurence of side effects.", "content": "The incidence of adverse effects is reported in a group of more than 400 patients treated with propranolol between 1964 and 1974. Severe side effects necessitating withdrawal of the drug were found in less than 10%; dose-limiting side effects were found in less than 15%. Tolerable side effects are studied in detail. Pharmacologically based side effects are recorded and ways of minimizing their frequency are discussed. The acceptability of propranolol in general terms and its advantages are assessed.", "contents": "Patient acceptability of propranolol and the occurence of side effects. The incidence of adverse effects is reported in a group of more than 400 patients treated with propranolol between 1964 and 1974. Severe side effects necessitating withdrawal of the drug were found in less than 10%; dose-limiting side effects were found in less than 15%. Tolerable side effects are studied in detail. Pharmacologically based side effects are recorded and ways of minimizing their frequency are discussed. The acceptability of propranolol in general terms and its advantages are assessed.", "PMID": 967773}
{"id": "pubmed23n0032_7132", "title": "Safeguards in the treatment of schizophrenia with propranolol.", "content": "Early results for an uncontrolled study of 555 patients with florid schizophrenia suggest that propranolol can be used safely in high dosage, and in a proportion of cases it appears to control schizophrenic symptoms. This method of treatment is now being submitted to controlled trial. Evdence from this uncontrolled study suggests that there was a therapeutic dose range in which symptoms steadily improved as a low dose was ineffective and a high dose, particularly if reached too rapidly, caused toxic effects. Rapid increases (400-800 mg) in the daily total intake when given in divided doses (4 to 10/day) produced gross toxic effects that included ataxia with unprotected falls, drop attacks, visual hallucinations, and confusional states. Severe toxic effects were uncommon when the dose was raised by regular, gradual increments (e.g. by 40-80 mg/day), when propranolol was given twice daily, when the dose was held steady as the patient started to improve, and when the daily total dose was reduced if the fall in pulse rate or blood pressure was excessive, or if there was evidence of toxicity. The observation of gradual, progressive improvement was the most valuable positive guide to the dose of propranolol. All schizophrenic symptoms remitted, at least temporarily, in 26 of 55 patients (in 15 of 17 patients who had been ill for less than one year and in 11 of 38 patients who had been ill for longer than a year). Patients who then stopped propranolol usually relapsed within hours or days.", "contents": "Safeguards in the treatment of schizophrenia with propranolol. Early results for an uncontrolled study of 555 patients with florid schizophrenia suggest that propranolol can be used safely in high dosage, and in a proportion of cases it appears to control schizophrenic symptoms. This method of treatment is now being submitted to controlled trial. Evdence from this uncontrolled study suggests that there was a therapeutic dose range in which symptoms steadily improved as a low dose was ineffective and a high dose, particularly if reached too rapidly, caused toxic effects. Rapid increases (400-800 mg) in the daily total intake when given in divided doses (4 to 10/day) produced gross toxic effects that included ataxia with unprotected falls, drop attacks, visual hallucinations, and confusional states. Severe toxic effects were uncommon when the dose was raised by regular, gradual increments (e.g. by 40-80 mg/day), when propranolol was given twice daily, when the dose was held steady as the patient started to improve, and when the daily total dose was reduced if the fall in pulse rate or blood pressure was excessive, or if there was evidence of toxicity. The observation of gradual, progressive improvement was the most valuable positive guide to the dose of propranolol. All schizophrenic symptoms remitted, at least temporarily, in 26 of 55 patients (in 15 of 17 patients who had been ill for less than one year and in 11 of 38 patients who had been ill for longer than a year). Patients who then stopped propranolol usually relapsed within hours or days.", "PMID": 967774}
{"id": "pubmed23n0032_7133", "title": "Port health problems.", "content": "As quarantine control of international travellers dies away, health screening of immigrants becomes more important. Port health controls attempt to identify the sick immigrant, thereby limiting disease spread among susceptibles and sparking early treatment. Port health controls also ease the impact of immigrants arriving at destinations by alerting relevant authorities so that they can begin health education procedures early, if they wish. Two problems arise: some destination authorities make little attempt to trace their immigrants and even those that do cannot find more than 70 per cent so notified. For receiving communities the most important disease the immigrant may carry or later develop is tuberculosis. In the indigenous population the incidence of this disease is decaying exponentially but, in communities of recent immigrants, may be stable or even rising. This means that in about ten years the great majority of new tuberculosis cases will be in recent immigrants, unless better preventive techniques are used. The overall trend is likely to be a fall in the number of cases, but the disproportion may provoke problems. What can be done? Assuming incoming immigrants are typical of their country of origin, many must enter Britain mantoux-negative, a fact supported by various destination authorities here which have performed testing. So, the logical solution is to ensure that all receive BCG vaccination, preferably before they migrate or otherwise as they enter Britain, and to ensure their babies born later in Britain also receive the protection of BCG vaccination. Consumption of medical services by the travelling public increases with the number of travellers and, as more people penetrate romantic but unhealthy areas formerly inaccessible, importation of communicable disease also increases. Many of these diseases could be prevented by good advice but, unfortunately, unfamiliarity blunts the edge of prophylaxis and diagnosis. Airport experience indicates that the general practitioner could help his patient more, particularly the overlander, by discussing sanitary precautions and suggesting anti-malarial drugs and immunization against typhoid and infectious hepatitis, when appropriate. Vaccination against smallpox, cholera or yellow fever may be obligatory and a valid certificate required; inoculation against diphtheria, poliomyelitis or tetanus may be sensible, and against rabies if the traveller may come into contact with animals abroad. Although complex queries about health hazards abroad should be addressed to such research centres as the Ross Institute, I suggest much practical advice can be obtained by phoning Heathrow health control 01-759 4361.", "contents": "Port health problems. As quarantine control of international travellers dies away, health screening of immigrants becomes more important. Port health controls attempt to identify the sick immigrant, thereby limiting disease spread among susceptibles and sparking early treatment. Port health controls also ease the impact of immigrants arriving at destinations by alerting relevant authorities so that they can begin health education procedures early, if they wish. Two problems arise: some destination authorities make little attempt to trace their immigrants and even those that do cannot find more than 70 per cent so notified. For receiving communities the most important disease the immigrant may carry or later develop is tuberculosis. In the indigenous population the incidence of this disease is decaying exponentially but, in communities of recent immigrants, may be stable or even rising. This means that in about ten years the great majority of new tuberculosis cases will be in recent immigrants, unless better preventive techniques are used. The overall trend is likely to be a fall in the number of cases, but the disproportion may provoke problems. What can be done? Assuming incoming immigrants are typical of their country of origin, many must enter Britain mantoux-negative, a fact supported by various destination authorities here which have performed testing. So, the logical solution is to ensure that all receive BCG vaccination, preferably before they migrate or otherwise as they enter Britain, and to ensure their babies born later in Britain also receive the protection of BCG vaccination. Consumption of medical services by the travelling public increases with the number of travellers and, as more people penetrate romantic but unhealthy areas formerly inaccessible, importation of communicable disease also increases. Many of these diseases could be prevented by good advice but, unfortunately, unfamiliarity blunts the edge of prophylaxis and diagnosis. Airport experience indicates that the general practitioner could help his patient more, particularly the overlander, by discussing sanitary precautions and suggesting anti-malarial drugs and immunization against typhoid and infectious hepatitis, when appropriate. Vaccination against smallpox, cholera or yellow fever may be obligatory and a valid certificate required; inoculation against diphtheria, poliomyelitis or tetanus may be sensible, and against rabies if the traveller may come into contact with animals abroad. Although complex queries about health hazards abroad should be addressed to such research centres as the Ross Institute, I suggest much practical advice can be obtained by phoning Heathrow health control 01-759 4361.", "PMID": 967779}
{"id": "pubmed23n0032_7134", "title": "Interchondral subluxation or \"clicking rib syndrome\".", "content": "A case history of the interchondral subluxation syndrome is presented, the anatomy as related to the symptoms discussed and the literature of this so-called \"clicking-rib syndrome\" reviewed. Unfortunately, this syndrome is not widely recognized but it is hoped that this article will bring it to the attention of those most likely to encounter it-the primary-care physician-and so save numerous unnecessary investigations and consultations. Once one is aware of its existence, the condition is easily diagnosed by careful history and examination alone.", "contents": "Interchondral subluxation or \"clicking rib syndrome\". A case history of the interchondral subluxation syndrome is presented, the anatomy as related to the symptoms discussed and the literature of this so-called \"clicking-rib syndrome\" reviewed. Unfortunately, this syndrome is not widely recognized but it is hoped that this article will bring it to the attention of those most likely to encounter it-the primary-care physician-and so save numerous unnecessary investigations and consultations. Once one is aware of its existence, the condition is easily diagnosed by careful history and examination alone.", "PMID": 967780}
{"id": "pubmed23n0032_7135", "title": "Effects of induction on the mother-baby relationship.", "content": "Even though a minority of mothers and babies benefit from the sequence of events which starts with induction, continues with active obstetric intervention, and culminates with the baby in the intensive care unit, it is questionable whether this justifies submitting the majority of women to what many of these mothers described as the misery of enforced separation from their infants, and whether we should permit modern technological innovations in the culture of childbirth to risk interfering with the basic human patterns of attachment between parents and their young.", "contents": "Effects of induction on the mother-baby relationship. Even though a minority of mothers and babies benefit from the sequence of events which starts with induction, continues with active obstetric intervention, and culminates with the baby in the intensive care unit, it is questionable whether this justifies submitting the majority of women to what many of these mothers described as the misery of enforced separation from their infants, and whether we should permit modern technological innovations in the culture of childbirth to risk interfering with the basic human patterns of attachment between parents and their young.", "PMID": 967782}
{"id": "pubmed23n0032_7136", "title": "Tuberculoid leprosy.", "content": "The clinical features and management of a case of major tuberculoid leprosy presenting in the United Kindom are described. The possibility of encountering tropical disease in one's practice should be remembered.", "contents": "Tuberculoid leprosy. The clinical features and management of a case of major tuberculoid leprosy presenting in the United Kindom are described. The possibility of encountering tropical disease in one's practice should be remembered.", "PMID": 967784}
{"id": "pubmed23n0032_7137", "title": "Forensic problems in practice VIII.-Sexual offences.", "content": "In general, the examination of alleged sexual offences is a combination of perception and experience in history taking and observation, of clinical skill in examination and of meticulous retention of material for forensic science examination. It is not a task to be undertaken lightly, especially as the doctor's actions and opinions may well become the subject of intensive cross-examination at any later court proceedings. The frequency with which unfounded allegations of rape or indecent assault are made by girls makes it all the more important to help separate the genuine from the spurious cases and medical evidence frequently plays a vital role in this direction.", "contents": "Forensic problems in practice VIII.-Sexual offences. In general, the examination of alleged sexual offences is a combination of perception and experience in history taking and observation, of clinical skill in examination and of meticulous retention of material for forensic science examination. It is not a task to be undertaken lightly, especially as the doctor's actions and opinions may well become the subject of intensive cross-examination at any later court proceedings. The frequency with which unfounded allegations of rape or indecent assault are made by girls makes it all the more important to help separate the genuine from the spurious cases and medical evidence frequently plays a vital role in this direction.", "PMID": 967785}
{"id": "pubmed23n0032_7138", "title": "[Health hazards to the anaesthesist].", "content": "During the past years there has been increasing concern regarding potential hazards to the anaesthetist presented by, e.g., anaesthetic agents, exposure to X-rays, virus hepatitis. Chronic exposure to inhalational anaesthetics, in particular, has been held responsible for a variety of complants and disorders. Investigations at the Technical University, Munich, have shown no appreciable difference in the sick rate between the staff of the Department of Anaesthetics and the Department of Medicine. Whereas exposure to X-radiation cannot be regarded as a health hazard provided the prescribed safety measures are adhered to, virus hepatitis still constitutes a major risk. There is also some evidence of an increased incidence of miscarriages amoung the female anaesthetic personnel. The causes are unknown. Halothane hepatitis is very rare and does not present a major risk.", "contents": "[Health hazards to the anaesthesist]. During the past years there has been increasing concern regarding potential hazards to the anaesthetist presented by, e.g., anaesthetic agents, exposure to X-rays, virus hepatitis. Chronic exposure to inhalational anaesthetics, in particular, has been held responsible for a variety of complants and disorders. Investigations at the Technical University, Munich, have shown no appreciable difference in the sick rate between the staff of the Department of Anaesthetics and the Department of Medicine. Whereas exposure to X-radiation cannot be regarded as a health hazard provided the prescribed safety measures are adhered to, virus hepatitis still constitutes a major risk. There is also some evidence of an increased incidence of miscarriages amoung the female anaesthetic personnel. The causes are unknown. Halothane hepatitis is very rare and does not present a major risk.", "PMID": 967786}
{"id": "pubmed23n0032_7139", "title": "[Danger of fire and explosion in anaesthetic and surgical departments].", "content": "Corresponding to the fact that flammable anaesthetics are still very seldron in use the possibility of fires an explosions has diminished; the frequency of accidents caused by flammable colonic gases and by desinficients is about unchanged whilst a new kind can and dose occur: Fires and (occassional) explosions with \"nonflammable\" anaesthetic technics. A survey about 16 cases (including oxygen-fires) should be of interest for surgeons and intensive-care-people as well as for anaesthetists.", "contents": "[Danger of fire and explosion in anaesthetic and surgical departments]. Corresponding to the fact that flammable anaesthetics are still very seldron in use the possibility of fires an explosions has diminished; the frequency of accidents caused by flammable colonic gases and by desinficients is about unchanged whilst a new kind can and dose occur: Fires and (occassional) explosions with \"nonflammable\" anaesthetic technics. A survey about 16 cases (including oxygen-fires) should be of interest for surgeons and intensive-care-people as well as for anaesthetists.", "PMID": 967787}
{"id": "pubmed23n0032_7140", "title": "Hygienic problems in anaesthesia and intensive care therapy.", "content": "Cross infection has become a serious risk to hospitalized patients. Potential sources of infection by anaesthetic apparatus and equipment and the danger arising from disregard of proper asepsis are discussed. Prophylactic and hygienic measures to minimize these hazards are reviewed. Since patients receiving intensive therapy are particularly are risk very high hygienic standards are a \"must\" in these units. The need for thoroughly and regularly checking all equipment for contamination is emphasized.", "contents": "Hygienic problems in anaesthesia and intensive care therapy. Cross infection has become a serious risk to hospitalized patients. Potential sources of infection by anaesthetic apparatus and equipment and the danger arising from disregard of proper asepsis are discussed. Prophylactic and hygienic measures to minimize these hazards are reviewed. Since patients receiving intensive therapy are particularly are risk very high hygienic standards are a \"must\" in these units. The need for thoroughly and regularly checking all equipment for contamination is emphasized.", "PMID": 967788}
{"id": "pubmed23n0032_7141", "title": "[Mild form of malignant hyperthermia? OR, ABNORMAL REACTION To succinyldicholine?].", "content": "In addition to two published cases of malignant hyperthermia (M.H.) 13 more cases have been observed in this institution since 1971, which are probably early reactions of the M.H. type. All 13 patients were children, 3-12 years of age, who developed a rigor of the jaw muscles after succinyldicholine and/or halothane within minutes after the induction of anesthesia. Further symptoms that could be observed regularly were a tachycardia of several hours duration and a marked elevation of the serum CPK-activity with a peak after 24 hours. Since the anesthesia was terminted immediately and the operations postponed as soon as a M.H. reaction was suspected, the rise in body temperature was minimal or absent and exceeded in no case 39 degrees C. Several children were operated upon later in neuroleptanalgesia (NLA) supplemented by nitrous oxide without any muscle relaxants. None of these children developed the aforementioned symptoms rigor, techycardia and CPK-elevation. One child had Alloferin in addition to NLA-nitrous oxide-anesthesia. This child had postoperatively a tachycardia lasting several hours and 24 hours later a serum CPK activity of 1500 U/1. Since this CPK activity is higher than in comparable cases (see fig. 1) it is concluded, that the nitrous oxide-NLA sequence appears to be safe for patients susceptible to M.H. Alloferin however must probably be regarded as a trigger agent for M.H.", "contents": "[Mild form of malignant hyperthermia? OR, ABNORMAL REACTION To succinyldicholine?]. In addition to two published cases of malignant hyperthermia (M.H.) 13 more cases have been observed in this institution since 1971, which are probably early reactions of the M.H. type. All 13 patients were children, 3-12 years of age, who developed a rigor of the jaw muscles after succinyldicholine and/or halothane within minutes after the induction of anesthesia. Further symptoms that could be observed regularly were a tachycardia of several hours duration and a marked elevation of the serum CPK-activity with a peak after 24 hours. Since the anesthesia was terminted immediately and the operations postponed as soon as a M.H. reaction was suspected, the rise in body temperature was minimal or absent and exceeded in no case 39 degrees C. Several children were operated upon later in neuroleptanalgesia (NLA) supplemented by nitrous oxide without any muscle relaxants. None of these children developed the aforementioned symptoms rigor, techycardia and CPK-elevation. One child had Alloferin in addition to NLA-nitrous oxide-anesthesia. This child had postoperatively a tachycardia lasting several hours and 24 hours later a serum CPK activity of 1500 U/1. Since this CPK activity is higher than in comparable cases (see fig. 1) it is concluded, that the nitrous oxide-NLA sequence appears to be safe for patients susceptible to M.H. Alloferin however must probably be regarded as a trigger agent for M.H.", "PMID": 967789}
{"id": "pubmed23n0032_7142", "title": "[Report on 2 cases of malignant hyperthermia with different clinical courses].", "content": "Two cases of malignant hyperthermia with different clinical courses are reported. The patients showed the classical signs of malignant hyperthermia consisting of tachycardia, tachypnoea, ocasional peripheral cyanosis, high body temperature as well as characteristic rise in serum enzymes. In one of the patients the symptoms were recognized early during the operation. The immediate commencement of therapy with ice-cooled. Ringer-Lactate-Solution, Procainmedication, Corticoids as well as physical body cooling favourably influenced the clinical course and the patient survived. In both cases the patients underwent succinylcholine and halothane anaesthesia, but the symptoms of the second patient appeared after the reduction of anaesthesia. In spite of vigorous therapy the hyperpyrexia resulted in heart arrest and death. Morphologically, both patients showed signs of preexistent myopathy with volumetric alterations of the muscle fibres, centralisation of the nuclei and acute muscle fibre necrosis. On the basis of the observed variable course, the various symptom complexes reported in the literature to data are  reviewed. A detailed discussion of the \"carrier problem\" and the available treatment possibilities is also made. Realising that malignant hyperthermia is an inheritable disease, prophylactic measures such as, f.i. the issue of medical certificates to the patient and his relatives are suggested.", "contents": "[Report on 2 cases of malignant hyperthermia with different clinical courses]. Two cases of malignant hyperthermia with different clinical courses are reported. The patients showed the classical signs of malignant hyperthermia consisting of tachycardia, tachypnoea, ocasional peripheral cyanosis, high body temperature as well as characteristic rise in serum enzymes. In one of the patients the symptoms were recognized early during the operation. The immediate commencement of therapy with ice-cooled. Ringer-Lactate-Solution, Procainmedication, Corticoids as well as physical body cooling favourably influenced the clinical course and the patient survived. In both cases the patients underwent succinylcholine and halothane anaesthesia, but the symptoms of the second patient appeared after the reduction of anaesthesia. In spite of vigorous therapy the hyperpyrexia resulted in heart arrest and death. Morphologically, both patients showed signs of preexistent myopathy with volumetric alterations of the muscle fibres, centralisation of the nuclei and acute muscle fibre necrosis. On the basis of the observed variable course, the various symptom complexes reported in the literature to data are  reviewed. A detailed discussion of the \"carrier problem\" and the available treatment possibilities is also made. Realising that malignant hyperthermia is an inheritable disease, prophylactic measures such as, f.i. the issue of medical certificates to the patient and his relatives are suggested.", "PMID": 967790}
{"id": "pubmed23n0032_7143", "title": "[Halothane-intoxication by failure of the halothane-vaporizer].", "content": "Regular  measurement of the Halothane-concentration delivered by the output of the vaporizer is necessary to avoid a clinically dangerous situation due to Halothane-overdosage.", "contents": "[Halothane-intoxication by failure of the halothane-vaporizer]. Regular  measurement of the Halothane-concentration delivered by the output of the vaporizer is necessary to avoid a clinically dangerous situation due to Halothane-overdosage.", "PMID": 967791}
{"id": "pubmed23n0032_7144", "title": "[Anti-G-suit-a facility of Neuroanaesthesia at operations in the sitting position].", "content": "The Anti-G-Suit- an over-all-air-pressure-garment over legs and bowel up to the xiphoid is proposed as a successful method to encounter acute postural decrease of blood pressure in neurosurgical patients. Increase of peripheral resistance and displacement of the circulating blood volume above the diaphragm enhance blood pressure which has fallen by the depressive effect on the vasomotor centre and the negative inotrope effect of narcotics.", "contents": "[Anti-G-suit-a facility of Neuroanaesthesia at operations in the sitting position]. The Anti-G-Suit- an over-all-air-pressure-garment over legs and bowel up to the xiphoid is proposed as a successful method to encounter acute postural decrease of blood pressure in neurosurgical patients. Increase of peripheral resistance and displacement of the circulating blood volume above the diaphragm enhance blood pressure which has fallen by the depressive effect on the vasomotor centre and the negative inotrope effect of narcotics.", "PMID": 967793}
{"id": "pubmed23n0032_7145", "title": "[Treatment of alkyl phosphate poisoning with purified serum cholinesterase (author's transl)].", "content": "A 26-years-old man injected himself intramuscularly with 3.75 gram of demeton methyl in an attempt to commit suicide. After 36 hours severe symptoms of poisoning developed with loss of consciousness and respiratory arrest. As symptomatic treatment failed to improve his condition has given injections of purified serum cholinesterase. This was followed by a rise in the cholin esterase levels and the increased activity was accompanied by a rapid and definite improvement in the clinical findings. The results clearly indicate the usefulness of this method of treatment. Transfusions of fresh blood to counteract the cholin esterase deficiency are inadvisable because of the risks of complications. Substitution therapy with serum cholinesterase must be accompanied by administration of large doses of atropine.", "contents": "[Treatment of alkyl phosphate poisoning with purified serum cholinesterase (author's transl)]. A 26-years-old man injected himself intramuscularly with 3.75 gram of demeton methyl in an attempt to commit suicide. After 36 hours severe symptoms of poisoning developed with loss of consciousness and respiratory arrest. As symptomatic treatment failed to improve his condition has given injections of purified serum cholinesterase. This was followed by a rise in the cholin esterase levels and the increased activity was accompanied by a rapid and definite improvement in the clinical findings. The results clearly indicate the usefulness of this method of treatment. Transfusions of fresh blood to counteract the cholin esterase deficiency are inadvisable because of the risks of complications. Substitution therapy with serum cholinesterase must be accompanied by administration of large doses of atropine.", "PMID": 967794}
{"id": "pubmed23n0032_7146", "title": "[Artificial respiration for the postoperative care after major surgery (author's transl)].", "content": "An analysis was made of the postoperative course of 158 cases of major surgery performed during 1971-1973. The operations comprised thoraco-abdominal resection of the oesophageal and cardia, abdominal duodenopancreatectomy, partial gastrectomy and enterectomy and partial hepatectomy. 63 per cent of the patients were given artificial respiration either prophylactically immediately after the operation (47 per cent) or at a later stage (53 per cent) on account of respiratory complications (arterial hypoxia below 55 mm Hg pO2). Postoperative mortality for the group of patients who did not have artificial respiration was 46 per cent, as compared with 32 per cent for the artifically ventilated patients - a difference of 14 per cent. The causes for this discrepancy, and especially the role of non-respiratory complications are discussed and the indications for prophylactic postoperative artificial respiration are reviewed.", "contents": "[Artificial respiration for the postoperative care after major surgery (author's transl)]. An analysis was made of the postoperative course of 158 cases of major surgery performed during 1971-1973. The operations comprised thoraco-abdominal resection of the oesophageal and cardia, abdominal duodenopancreatectomy, partial gastrectomy and enterectomy and partial hepatectomy. 63 per cent of the patients were given artificial respiration either prophylactically immediately after the operation (47 per cent) or at a later stage (53 per cent) on account of respiratory complications (arterial hypoxia below 55 mm Hg pO2). Postoperative mortality for the group of patients who did not have artificial respiration was 46 per cent, as compared with 32 per cent for the artifically ventilated patients - a difference of 14 per cent. The causes for this discrepancy, and especially the role of non-respiratory complications are discussed and the indications for prophylactic postoperative artificial respiration are reviewed.", "PMID": 967795}
{"id": "pubmed23n0032_7147", "title": "[The influence of enflurane on the haemodynamics in the pulmonary vasculary system (author's transl)].", "content": "The influence of enflurane on the haemodynamics of the main and pulmonary vasculary system was studied in young healthy patients. The results showed no influence in heart frequency and mean arterial pressure, a slight fall in certain output and a significant increase in mean pulmonary arterial pressure. There is no certain explanation for the pathophysiological mechanism in pulmonary vasculary system caused by enflurane. According to our opinion these results are to discuss as a cause of a decrease of left ventricular work and a regulative vasoconstriction in the pulmonary vasculary system.", "contents": "[The influence of enflurane on the haemodynamics in the pulmonary vasculary system (author's transl)]. The influence of enflurane on the haemodynamics of the main and pulmonary vasculary system was studied in young healthy patients. The results showed no influence in heart frequency and mean arterial pressure, a slight fall in certain output and a significant increase in mean pulmonary arterial pressure. There is no certain explanation for the pathophysiological mechanism in pulmonary vasculary system caused by enflurane. According to our opinion these results are to discuss as a cause of a decrease of left ventricular work and a regulative vasoconstriction in the pulmonary vasculary system.", "PMID": 967796}
{"id": "pubmed23n0032_7148", "title": "[Catheterization of the vena cava superior via the internal jugular vein (author's transl)].", "content": "A method is described of introducing a catheter into the vena cava superior via the internal jugular vein. Possible complications and the experience gained with the method in 260 cases are discussed.", "contents": "[Catheterization of the vena cava superior via the internal jugular vein (author's transl)]. A method is described of introducing a catheter into the vena cava superior via the internal jugular vein. Possible complications and the experience gained with the method in 260 cases are discussed.", "PMID": 967797}
{"id": "pubmed23n0032_7149", "title": "[Verifying right position of central venous catheters by intracardial ecg derivation (author's transl)].", "content": "It is reported on the electrocardiographical method for the location of the position of central-venous catheter. The advantages of this method compared to the usual radiological method: immediate correction is possible no additional apparat is needed.", "contents": "[Verifying right position of central venous catheters by intracardial ecg derivation (author's transl)]. It is reported on the electrocardiographical method for the location of the position of central-venous catheter. The advantages of this method compared to the usual radiological method: immediate correction is possible no additional apparat is needed.", "PMID": 967798}
{"id": "pubmed23n0032_7150", "title": "[Dopamin-therapy for acute postoperative renal failure (author's transl)].", "content": "In 10 out 14 patients with acute postoperative renal failure the administration of a dopamin-drip (175-350 mcg/min) resulted in an increase in urinary output more than 30 ml/h. But there was no decrease of the high lethality by postoperative renal failure.", "contents": "[Dopamin-therapy for acute postoperative renal failure (author's transl)]. In 10 out 14 patients with acute postoperative renal failure the administration of a dopamin-drip (175-350 mcg/min) resulted in an increase in urinary output more than 30 ml/h. But there was no decrease of the high lethality by postoperative renal failure.", "PMID": 967799}
{"id": "pubmed23n0032_7151", "title": "[Abdominal and cremaster reflexes after trauma-induced brain death (author's transl)].", "content": "The case is reported of a 42-years-old man who was admitted to hospital with a head trauma following a fall and died 12 hours later. Although irreversible loss of cerebral function had been established neurologically, electoencephalographically, angiographically (carotid and vertebral angiography) and scintigraphically abdominal and cremaster reflexes could still be elicited. The neurophysiological conditions for the reappearance of these reflexes are discussed.", "contents": "[Abdominal and cremaster reflexes after trauma-induced brain death (author's transl)]. The case is reported of a 42-years-old man who was admitted to hospital with a head trauma following a fall and died 12 hours later. Although irreversible loss of cerebral function had been established neurologically, electoencephalographically, angiographically (carotid and vertebral angiography) and scintigraphically abdominal and cremaster reflexes could still be elicited. The neurophysiological conditions for the reappearance of these reflexes are discussed.", "PMID": 967800}
{"id": "pubmed23n0032_7152", "title": "[Prolonged extracorporeal membrane oxygenation in the treatment of burns-induced shock lung (authors transl)].", "content": "A case of third-degree burns involving 35 per cent of the body surface is reported with occurred in a 28-year-old man. On the 6th day progressive pulmonary insufficiency developed which 3 days later necessitated extracorporeal membrane oxygenation. Oxygen diffusion capacity gradually improved and the patients resumed spontaneous respiration with oxygen enriched air. He died 5 days after membrane oxygenation was discontinued due to massive haemorrhages from an infection at the site of the femoral by-pass. The technique adopted for the perfusion, which lasted 83 hours, is described.", "contents": "[Prolonged extracorporeal membrane oxygenation in the treatment of burns-induced shock lung (authors transl)]. A case of third-degree burns involving 35 per cent of the body surface is reported with occurred in a 28-year-old man. On the 6th day progressive pulmonary insufficiency developed which 3 days later necessitated extracorporeal membrane oxygenation. Oxygen diffusion capacity gradually improved and the patients resumed spontaneous respiration with oxygen enriched air. He died 5 days after membrane oxygenation was discontinued due to massive haemorrhages from an infection at the site of the femoral by-pass. The technique adopted for the perfusion, which lasted 83 hours, is described.", "PMID": 967804}
{"id": "pubmed23n0032_7153", "title": "The effects of ozone on the respiratory epithelium and alveolar macrophages of mice. I. Interferon production.", "content": "The effects of 0.8 ppm ozone on the capacity of the tracheal epithelium and alveolar macrophages of mice to produce interferon in vitro was studied. Exposure of mice to ozone for a period of 11 days or more affected the capacity of the tracheal epithelial cells in vitro to produce interferon. The inability of the tracheal epithelium in vitro to produce interferon was not due to the inhibition in the release of intracellular interferon but to an inhibition in the production of interferon. There was a complete recovery of the ability of tracheal epithelium to respond to interferon inducers after the mice were returned to ambient air 24 days post ozone exposure. However, ozone did not seem to have any affect on the capacity of the alveolar macrophages to produce interferon in vitro.", "contents": "The effects of ozone on the respiratory epithelium and alveolar macrophages of mice. I. Interferon production. The effects of 0.8 ppm ozone on the capacity of the tracheal epithelium and alveolar macrophages of mice to produce interferon in vitro was studied. Exposure of mice to ozone for a period of 11 days or more affected the capacity of the tracheal epithelial cells in vitro to produce interferon. The inability of the tracheal epithelium in vitro to produce interferon was not due to the inhibition in the release of intracellular interferon but to an inhibition in the production of interferon. There was a complete recovery of the ability of tracheal epithelium to respond to interferon inducers after the mice were returned to ambient air 24 days post ozone exposure. However, ozone did not seem to have any affect on the capacity of the alveolar macrophages to produce interferon in vitro.", "PMID": 967873}
{"id": "pubmed23n0032_7154", "title": "Endotoxin-stimulated spleen cells: dissociation between DNA synthesis and IgM production at the cellular level.", "content": "LPS stimulation of mouse spleen cells in the presence of Hu resulted in almost total suppression of [3H]thymidine incorporation without affecting the percentage of cells induced to produce IgM. Utilizing a method which permitted the simultaneous measurement of IgM production and [3H]thymidine incorporation in individual cells, it was demonstrated directly that LPS stimulation of IgM production can occur in the absence of DNA synthesis.", "contents": "Endotoxin-stimulated spleen cells: dissociation between DNA synthesis and IgM production at the cellular level. LPS stimulation of mouse spleen cells in the presence of Hu resulted in almost total suppression of [3H]thymidine incorporation without affecting the percentage of cells induced to produce IgM. Utilizing a method which permitted the simultaneous measurement of IgM production and [3H]thymidine incorporation in individual cells, it was demonstrated directly that LPS stimulation of IgM production can occur in the absence of DNA synthesis.", "PMID": 967874}
{"id": "pubmed23n0032_7155", "title": "Kinetics of immunoglobulin transport into canine bronchial secretions.", "content": "Radiolabeled 125I Preparations of canine IgG, IgM, and F(ab')2 fragments of IgG were injected intramuscularly into normal dogs to quantitate the transfer of immunoglobulins from the intravascular space into the lower respiratory tract and to observe the clearance of these proteins from lung secretions. Respiratory fluids were sampled by serial broncho-alveolar lavages during a 3-4 week interval. Only a small portion (0.15%) of the passively administered IgG was recovered in respiratory specimens indicating that minimal transfer of humoral IgG occurs under normal conditions. Alteration of the IgG molecule by removal of the Fc portion almost eliminates its penetration into lung secretions. Furthermore, IgM, which is not a prominent component of normal lung secretions, is not detected in these secretions following passive intramuscular injection.", "contents": "Kinetics of immunoglobulin transport into canine bronchial secretions. Radiolabeled 125I Preparations of canine IgG, IgM, and F(ab')2 fragments of IgG were injected intramuscularly into normal dogs to quantitate the transfer of immunoglobulins from the intravascular space into the lower respiratory tract and to observe the clearance of these proteins from lung secretions. Respiratory fluids were sampled by serial broncho-alveolar lavages during a 3-4 week interval. Only a small portion (0.15%) of the passively administered IgG was recovered in respiratory specimens indicating that minimal transfer of humoral IgG occurs under normal conditions. Alteration of the IgG molecule by removal of the Fc portion almost eliminates its penetration into lung secretions. Furthermore, IgM, which is not a prominent component of normal lung secretions, is not detected in these secretions following passive intramuscular injection.", "PMID": 967875}
{"id": "pubmed23n0032_7156", "title": "Studies on the blood clotting and fibrinolytic system in the plasma from a sei (baleen) whale.", "content": "Blood clotting and fibrinolytic systems were studied in the plasma of a sei whale (Balaenoptera borealis). The sei whale belongs to the suborder baleen whales of the order Cetacea. Whale plasma had a greatly prolonged kaolin-activated partial thromboplastin time and was deficient in Hageman factor (factor XII), Fletcher factor (a plasma prekallikrein), and PTA (factor XI). All other clotting factor activities were present in amounts comparable to that of normal human plasma. Whale plasminogen was activated by human urokinase, but not by streptokinase. Whale plasma contained inhibitory activities against thrombin, activated Stuart factor, activated PTA, activated Fletcher factor, and plasmin.", "contents": "Studies on the blood clotting and fibrinolytic system in the plasma from a sei (baleen) whale. Blood clotting and fibrinolytic systems were studied in the plasma of a sei whale (Balaenoptera borealis). The sei whale belongs to the suborder baleen whales of the order Cetacea. Whale plasma had a greatly prolonged kaolin-activated partial thromboplastin time and was deficient in Hageman factor (factor XII), Fletcher factor (a plasma prekallikrein), and PTA (factor XI). All other clotting factor activities were present in amounts comparable to that of normal human plasma. Whale plasminogen was activated by human urokinase, but not by streptokinase. Whale plasma contained inhibitory activities against thrombin, activated Stuart factor, activated PTA, activated Fletcher factor, and plasmin.", "PMID": 967876}
{"id": "pubmed23n0032_7157", "title": "Isolation and partial characterization of a major basic protein from rat eosinophil granules.", "content": "The rat eosinophil granule possesses a major basic protein (MBP) similar to that previously isolated from human and guinea pig eosinophils. This conclusion is based on demonstration that the major protein of the rat eosinophil granule is a low molecular weight, highly basic material with an amino acid composition similar to that of the human and guinea pig MBP.", "contents": "Isolation and partial characterization of a major basic protein from rat eosinophil granules. The rat eosinophil granule possesses a major basic protein (MBP) similar to that previously isolated from human and guinea pig eosinophils. This conclusion is based on demonstration that the major protein of the rat eosinophil granule is a low molecular weight, highly basic material with an amino acid composition similar to that of the human and guinea pig MBP.", "PMID": 967878}
{"id": "pubmed23n0032_7158", "title": "A radioimmunoassay for serum calcitonin in the rat.", "content": "A radioimmunoassay for serum calcitonin in the rat is described. Materials for assay of human calcitonin are employed. The sensitivity of the assay is 3 pg and corresponds to a lower limit of detection of 60 pg/ml for peripheral serum. The normal range is up to 650 pg/ml in rats weighing between 125 and 200 g. Serum calcitonin is not detectable in thyroparathyroidectomized animals. It increases and varies directly with the serum calcium after intraperitoneal administration of the ion. Values for serum calcitonin in male and female rats are comparable under basal conditions and after calcium. The radioimmunoassay should allow the further characterization of calcitonin secretion in response to physiologic and pathophysiologic stimuli in this species.", "contents": "A radioimmunoassay for serum calcitonin in the rat. A radioimmunoassay for serum calcitonin in the rat is described. Materials for assay of human calcitonin are employed. The sensitivity of the assay is 3 pg and corresponds to a lower limit of detection of 60 pg/ml for peripheral serum. The normal range is up to 650 pg/ml in rats weighing between 125 and 200 g. Serum calcitonin is not detectable in thyroparathyroidectomized animals. It increases and varies directly with the serum calcium after intraperitoneal administration of the ion. Values for serum calcitonin in male and female rats are comparable under basal conditions and after calcium. The radioimmunoassay should allow the further characterization of calcitonin secretion in response to physiologic and pathophysiologic stimuli in this species.", "PMID": 967879}
{"id": "pubmed23n0032_7159", "title": "Glutathione peroxidase activity and glutathione concentration in genetically dystrophic mice.", "content": "The present studies were conducted to determine whether inherited muscular dystrophy in the 129/ReJ-dy mouse was associated with differences in specific activity, substrate availability, or apparent Km of glutathione peroxidase. The results indicate that glutathione peroxidase is elevated in skeletal muscle of mice with genetic muscular dystrophy when the activity is expressed on a protein basis. This elevation precedes the development of severe paralysis since muscles from the fore legs showed increased enzyme activity as early as the more severely affected hind legs. There was no difference in glutathione peroxidase activity in tissues other than skeletal muscle. GSH concentration was elevated in muscle and normal in other tissues of dystrophic mice, showing that adequate substrate was available to the enzyme. The apparent Km for cumene hydroperoxide was also similar for muscle of normal and dystrophic mice. This report provides further evidence that mice with dystrophia muscularis have a functional glutathione peroxidase system in all tissues including skeletal muscle, and that a defect in this in vivo protective system is apparently not a contributing factor in the pathology of the disease.", "contents": "Glutathione peroxidase activity and glutathione concentration in genetically dystrophic mice. The present studies were conducted to determine whether inherited muscular dystrophy in the 129/ReJ-dy mouse was associated with differences in specific activity, substrate availability, or apparent Km of glutathione peroxidase. The results indicate that glutathione peroxidase is elevated in skeletal muscle of mice with genetic muscular dystrophy when the activity is expressed on a protein basis. This elevation precedes the development of severe paralysis since muscles from the fore legs showed increased enzyme activity as early as the more severely affected hind legs. There was no difference in glutathione peroxidase activity in tissues other than skeletal muscle. GSH concentration was elevated in muscle and normal in other tissues of dystrophic mice, showing that adequate substrate was available to the enzyme. The apparent Km for cumene hydroperoxide was also similar for muscle of normal and dystrophic mice. This report provides further evidence that mice with dystrophia muscularis have a functional glutathione peroxidase system in all tissues including skeletal muscle, and that a defect in this in vivo protective system is apparently not a contributing factor in the pathology of the disease.", "PMID": 967880}
{"id": "pubmed23n0032_7160", "title": "Seroepidemiologic investigations of human hepatitis caused by A, B, and a possible third virus.", "content": "Seroepidemiologic studies were made of normal subjects in populations in the United States and Costa Rica and in family outbreaks of hepatitis in Costa Rica. Hepatitis A affected a majority of children of very young age in Costa Rica, while such experience in persons of high socioeconomic status in the United States did not occur before middle life. Persons of low socioeconomic status (paid plasma donors) and residents and attendants of institutions for the mentally retarded showed a far greater incidence of hepatitis A antibody than did their counterparts in the open community. Hepatitis A and B epidemics occurred in families in Costa Rica with rapid spread to other susceptible members of the group. The disease was clinically apparent in roughly half the cases, whether the responsible agent be hepatitis A or B. Five cases of nonhepatitis A or B (hypothetical hepatitis C) were found and all but one of them were subclinical.", "contents": "Seroepidemiologic investigations of human hepatitis caused by A, B, and a possible third virus. Seroepidemiologic studies were made of normal subjects in populations in the United States and Costa Rica and in family outbreaks of hepatitis in Costa Rica. Hepatitis A affected a majority of children of very young age in Costa Rica, while such experience in persons of high socioeconomic status in the United States did not occur before middle life. Persons of low socioeconomic status (paid plasma donors) and residents and attendants of institutions for the mentally retarded showed a far greater incidence of hepatitis A antibody than did their counterparts in the open community. Hepatitis A and B epidemics occurred in families in Costa Rica with rapid spread to other susceptible members of the group. The disease was clinically apparent in roughly half the cases, whether the responsible agent be hepatitis A or B. Five cases of nonhepatitis A or B (hypothetical hepatitis C) were found and all but one of them were subclinical.", "PMID": 967881}
{"id": "pubmed23n0032_7161", "title": "Effects of glucose or insulin on myocardial performance in endotoxin shock.", "content": "Past studies reported by this laboratory have documented myocardial dysfunction and progressively developing hypoglycemia in canine endotoxin shock. The purpose of the present study was to determine the effects of glucose concentrations and insulin infusions on myocardial performance following endotoxin administration. Experiments were carried out on isolated, working canine left ventricular heart preparations exchanging blood with intact dogs. Myocardial function was evaluated following endotoxin and correlated with concentrations of glucose and effects of insulin infusion. Cardiac dysfunction occurred within 2-4 hr postendotoxin and the degree of malfunction was not related to arterial blood glucose concentrations. Maintaining blood glucose at control, preshock, levels by infusion of 50% glucose did not prevent myocardial dysfunction as evidenced by elevations of left ventricular end diastolic pressure, and depressed power. Infusions of insulin reversed cardiac failure and maintained normal performance in spite of wide ranges in glucose concentration (5-120 mg%). Findings suggest that myocardial dysfunction is not precipitated or enhanced by the hypoglycemia of endotoxin shock. The beneficial actions of infused insulin on cardiac performance appear to be elicited on the basis of mechanisms other than myocardial glucose transport.", "contents": "Effects of glucose or insulin on myocardial performance in endotoxin shock. Past studies reported by this laboratory have documented myocardial dysfunction and progressively developing hypoglycemia in canine endotoxin shock. The purpose of the present study was to determine the effects of glucose concentrations and insulin infusions on myocardial performance following endotoxin administration. Experiments were carried out on isolated, working canine left ventricular heart preparations exchanging blood with intact dogs. Myocardial function was evaluated following endotoxin and correlated with concentrations of glucose and effects of insulin infusion. Cardiac dysfunction occurred within 2-4 hr postendotoxin and the degree of malfunction was not related to arterial blood glucose concentrations. Maintaining blood glucose at control, preshock, levels by infusion of 50% glucose did not prevent myocardial dysfunction as evidenced by elevations of left ventricular end diastolic pressure, and depressed power. Infusions of insulin reversed cardiac failure and maintained normal performance in spite of wide ranges in glucose concentration (5-120 mg%). Findings suggest that myocardial dysfunction is not precipitated or enhanced by the hypoglycemia of endotoxin shock. The beneficial actions of infused insulin on cardiac performance appear to be elicited on the basis of mechanisms other than myocardial glucose transport.", "PMID": 967882}
{"id": "pubmed23n0032_7162", "title": "Plasma glucagon after total resection of the pancreas in man.", "content": "Serial measurements of plasma \"true glucagon\" (PG) and of glucagon-like immunoreactive materials (GLI) were carried out during and after total resection of the pancreas in a 62-year-old man with calcified pancreatitis. The postoperative course of this patient was uneventful and diabetes was well controlled. PG disappeared from the blood within 30 min after resection of the pancreas. In spite of the evidence that no pancreatic tissue was present in the abdomen, PG was detected again in the blood from 18 hr after total pancreatectomy until the ninth postoperative day. However, plasma PG did not rise following infusion of arginine during the fourteenth postoperative week. After an initial decrease, plasma GLI rose abruptly on the second postoperative day and remained elevated thereafter. The fluctuations of plasma PG and GLI were not parallel.", "contents": "Plasma glucagon after total resection of the pancreas in man. Serial measurements of plasma \"true glucagon\" (PG) and of glucagon-like immunoreactive materials (GLI) were carried out during and after total resection of the pancreas in a 62-year-old man with calcified pancreatitis. The postoperative course of this patient was uneventful and diabetes was well controlled. PG disappeared from the blood within 30 min after resection of the pancreas. In spite of the evidence that no pancreatic tissue was present in the abdomen, PG was detected again in the blood from 18 hr after total pancreatectomy until the ninth postoperative day. However, plasma PG did not rise following infusion of arginine during the fourteenth postoperative week. After an initial decrease, plasma GLI rose abruptly on the second postoperative day and remained elevated thereafter. The fluctuations of plasma PG and GLI were not parallel.", "PMID": 967883}
{"id": "pubmed23n0032_7163", "title": "Effect of skin or muscle incisions or the loss of blood on serum leucogenenol level.", "content": "It has been found that damage to a tissue of a rabbit or a rat, such as results from a skin incision or an incision through the skin and muscles into the abdominal cavity, is followed 24 hr later by a significant increase in the concentration of leucogenenol in the animal's serum. Likewise, loss of approximately one-quarter to one-half of the blood in the circulation of rabbits or rats causes an increase 24 hr later in the animals' serum leucogenenol concentration.", "contents": "Effect of skin or muscle incisions or the loss of blood on serum leucogenenol level. It has been found that damage to a tissue of a rabbit or a rat, such as results from a skin incision or an incision through the skin and muscles into the abdominal cavity, is followed 24 hr later by a significant increase in the concentration of leucogenenol in the animal's serum. Likewise, loss of approximately one-quarter to one-half of the blood in the circulation of rabbits or rats causes an increase 24 hr later in the animals' serum leucogenenol concentration.", "PMID": 967884}
{"id": "pubmed23n0032_7164", "title": "Role of the renin-angiotensin system in dogs with perinephritis hypertension.", "content": "Cellophane perinephritis hypertension was produced in four dogs, while five additional dogs served as normotensive controls. A competitive antagonist of angiotensin II, 1-sarcosine-8-alanine angiotensin II, was infused iv into these conscious dogs at a rate of 6 mug/min/kg of body weight for 45 min. Arterial pressure averaged 170 +/- 11 (SEM) mm Hg in the dogs with perinephritic hypertension, and was not altered significantly during infusion of the angiotensin antagonist. In the normal dogs the arterial pressure averaged 100 +/- 10 mm Hg and likewise, did not change during administration of the angiotensin analog. Plasma renin activity values were essentially the same in these two groups of dogs and did not change during infusion of the angiotensin antagonist. These studies provide strong evidence that the renin-angiotensin system is not involved in maintaining the elevated arterial pressure in dogs with chronic hypertension produced by cellophane perinephritis.", "contents": "Role of the renin-angiotensin system in dogs with perinephritis hypertension. Cellophane perinephritis hypertension was produced in four dogs, while five additional dogs served as normotensive controls. A competitive antagonist of angiotensin II, 1-sarcosine-8-alanine angiotensin II, was infused iv into these conscious dogs at a rate of 6 mug/min/kg of body weight for 45 min. Arterial pressure averaged 170 +/- 11 (SEM) mm Hg in the dogs with perinephritic hypertension, and was not altered significantly during infusion of the angiotensin antagonist. In the normal dogs the arterial pressure averaged 100 +/- 10 mm Hg and likewise, did not change during administration of the angiotensin analog. Plasma renin activity values were essentially the same in these two groups of dogs and did not change during infusion of the angiotensin antagonist. These studies provide strong evidence that the renin-angiotensin system is not involved in maintaining the elevated arterial pressure in dogs with chronic hypertension produced by cellophane perinephritis.", "PMID": 967886}
{"id": "pubmed23n0032_7165", "title": "Direct stimulation of renin release by calcium.", "content": "Calcium directly stimulates renin release from rat kidney slices previously treated with calcium-free medium. The stimulant effect of calcium (0.5 or 6.0 mM) is not seen without the period of calcium depletion. The stimulant effect of calcium is still present in sodium-free medium but is reduced when the incubation is performed at 20 degrees instead of 37 degrees. The results suggest that the underlying mechanism of renin release may be comparable to that of catecholamine release, involving calcium-dependent and energy-dependent steps.", "contents": "Direct stimulation of renin release by calcium. Calcium directly stimulates renin release from rat kidney slices previously treated with calcium-free medium. The stimulant effect of calcium (0.5 or 6.0 mM) is not seen without the period of calcium depletion. The stimulant effect of calcium is still present in sodium-free medium but is reduced when the incubation is performed at 20 degrees instead of 37 degrees. The results suggest that the underlying mechanism of renin release may be comparable to that of catecholamine release, involving calcium-dependent and energy-dependent steps.", "PMID": 967887}
{"id": "pubmed23n0032_7166", "title": "Effect of leukocytic endogenous mediator (LEM) on zinc absorption in the rat.", "content": "Leukocytic endogenous mediator (LEM) was shown to produce a significant increase in the intestinal absorption of zinc within 7 hr after its administration to normal rats. Consistent with earlier studies, LEM also produced a significant decrease in the plasma zinc concentration and an increased hepatic uptake of this metal which further demonstrates that LEM may be a key intermediate in altering zinc homeostasis during inflammation.", "contents": "Effect of leukocytic endogenous mediator (LEM) on zinc absorption in the rat. Leukocytic endogenous mediator (LEM) was shown to produce a significant increase in the intestinal absorption of zinc within 7 hr after its administration to normal rats. Consistent with earlier studies, LEM also produced a significant decrease in the plasma zinc concentration and an increased hepatic uptake of this metal which further demonstrates that LEM may be a key intermediate in altering zinc homeostasis during inflammation.", "PMID": 967889}
{"id": "pubmed23n0032_7167", "title": "Immunogenic properties of a nonproducer malignant tumor induced by murine sarcoma virus.", "content": "A cloned cell line H-11 (HP) derived from an MSV-induced neoplasm (a hemangiosarcoma) was found to possess virus-specific tumor rejection antigen(s). The specific nature of the immune response was established through the use of a polyoma virus-induced neoplasm no. 89 both in cross immunization and cross challenge experiments. Virus antigens or surface antigens associated with virus production are not responsible for tumor rejection since virus production could not be detected, nor were any viral fingerprints of MSV found by the several assays used.", "contents": "Immunogenic properties of a nonproducer malignant tumor induced by murine sarcoma virus. A cloned cell line H-11 (HP) derived from an MSV-induced neoplasm (a hemangiosarcoma) was found to possess virus-specific tumor rejection antigen(s). The specific nature of the immune response was established through the use of a polyoma virus-induced neoplasm no. 89 both in cross immunization and cross challenge experiments. Virus antigens or surface antigens associated with virus production are not responsible for tumor rejection since virus production could not be detected, nor were any viral fingerprints of MSV found by the several assays used.", "PMID": 967890}
{"id": "pubmed23n0032_7168", "title": "Kinetics of the rapid action of interferon.", "content": "The present study was undertaken to examine some factors which lead to early-appearing resistance in human cells treated with interferon. It was previously shown that two conditions required for rapid development of resistance were continuous maintenance of cultures at 37 degrees and use of more than 10 units/ml of interferon. The decay kinetics of the established resistance appear to be approximately the same whether the resistance was induced under conditions favoring rapid or slow development. With the use of actinomycin D it was shown that the mRNA for the antiviral protein is produced between 30 and 45 min after the first contact with interferon. Ruled out were the possibilities that a priming action of interferon and a newly synthesized intermediary protein were necessary for rapid development of resistance.", "contents": "Kinetics of the rapid action of interferon. The present study was undertaken to examine some factors which lead to early-appearing resistance in human cells treated with interferon. It was previously shown that two conditions required for rapid development of resistance were continuous maintenance of cultures at 37 degrees and use of more than 10 units/ml of interferon. The decay kinetics of the established resistance appear to be approximately the same whether the resistance was induced under conditions favoring rapid or slow development. With the use of actinomycin D it was shown that the mRNA for the antiviral protein is produced between 30 and 45 min after the first contact with interferon. Ruled out were the possibilities that a priming action of interferon and a newly synthesized intermediary protein were necessary for rapid development of resistance.", "PMID": 967891}
{"id": "pubmed23n0032_7169", "title": "Spontaneous development of gluconeogenesis in fetal rat livers during incubation in organ culture.", "content": "Freshly isolated fetal liver explants in organ culture did not convert L-[14C]alanine or L-[14C]lactate to carbohydrate, but L-[14C]serine and D-[14C]glycerol were both transformed. When explants were subjected to 42 hr of preliminary incubation without supplements, followed by transfer to fresh medium with added precursor, all four substrates underwent gluconeogenic transformation. It was concluded that the ability of fetal rat liver in organ culture to convert alanine and lactate to carbohydrate evolves slowly, but the conversion of glycerol, and to a lesser extent serine, to glucose and glycogen is initiated immediately.", "contents": "Spontaneous development of gluconeogenesis in fetal rat livers during incubation in organ culture. Freshly isolated fetal liver explants in organ culture did not convert L-[14C]alanine or L-[14C]lactate to carbohydrate, but L-[14C]serine and D-[14C]glycerol were both transformed. When explants were subjected to 42 hr of preliminary incubation without supplements, followed by transfer to fresh medium with added precursor, all four substrates underwent gluconeogenic transformation. It was concluded that the ability of fetal rat liver in organ culture to convert alanine and lactate to carbohydrate evolves slowly, but the conversion of glycerol, and to a lesser extent serine, to glucose and glycogen is initiated immediately.", "PMID": 967892}
{"id": "pubmed23n0032_7170", "title": "Environmental effects on glutathione-insulin transhydrogenase in rat liver.", "content": "The dietary and hormonal regulation of the level of glutathione-insulin tranhydrogenase in rat liver was investigated in these studies. In order to make valid comparisons, the assay of glutathione-insulin transhydrogenase was performed at near zero-order kinetics wherein enzyme rate was proportional to enzyme amount. Changing the protein content of the diet or administration of glucagon or cortisone did not significantly affect the specific activity of glutathione-insulin transhydrogenase in microsomes from rat liver. However, the Vmax and Km of this enzyme in the livers of adrenalectomized rats were increased three-and fourfold over these values in microsomes from normal liver. Administration of cortisone resulted in a return to the normal kinetic constants of microsomal GIT within 4 hr.", "contents": "Environmental effects on glutathione-insulin transhydrogenase in rat liver. The dietary and hormonal regulation of the level of glutathione-insulin tranhydrogenase in rat liver was investigated in these studies. In order to make valid comparisons, the assay of glutathione-insulin transhydrogenase was performed at near zero-order kinetics wherein enzyme rate was proportional to enzyme amount. Changing the protein content of the diet or administration of glucagon or cortisone did not significantly affect the specific activity of glutathione-insulin transhydrogenase in microsomes from rat liver. However, the Vmax and Km of this enzyme in the livers of adrenalectomized rats were increased three-and fourfold over these values in microsomes from normal liver. Administration of cortisone resulted in a return to the normal kinetic constants of microsomal GIT within 4 hr.", "PMID": 967893}
{"id": "pubmed23n0032_7171", "title": "Developmental studies on glucosamine metabolism.", "content": "Changes in hepatic hexosamine metabolism and serum seromucoid concentrations during postnatal development were investigated in the rat. A relatively low activity of hepatic L-glutamine: D-fructose-6-phosphate aminotransferase (AT) was observed within 24 hr of birth. This rapidly increased to a maximum at about 2 weeks of age, followed by a decline to adult levels after another 2 weeks. The developmental pattern of hepatic UDP-N-acetylglucosamine 2'-epimerase (EP) closely resembled that of AT. It was relatively low in the newborn, increased to a maximum at about 2 weeks and then declined to adult values after another 2 weeks. The serum seromucoid concentration was low in the new born rats and then increased gradually with development to adult levels at about 50 days. The hepatic concentrations of UDP-GlcNAC and CMP-NANA both were relatively high at 24 hr after birth compared to values observed at 50 days. The developmental pattern observed for both over this time period was quite similar and was almost the reciprocal to that for the seromucoid fraction. After 50 days the concentration of UDP-GlcNAc remained constant at adult levels as did that of the seromucoid fraction, but the concentration of CMP-NANA showed a marked increase. It is suggested that the hepatic concentrations of these amino-sugar nucleotides during development primarily reflect the demand for the hepatic synthesis and secretion of plasma glycoproteins as reflected in the seromucoid fraction.", "contents": "Developmental studies on glucosamine metabolism. Changes in hepatic hexosamine metabolism and serum seromucoid concentrations during postnatal development were investigated in the rat. A relatively low activity of hepatic L-glutamine: D-fructose-6-phosphate aminotransferase (AT) was observed within 24 hr of birth. This rapidly increased to a maximum at about 2 weeks of age, followed by a decline to adult levels after another 2 weeks. The developmental pattern of hepatic UDP-N-acetylglucosamine 2'-epimerase (EP) closely resembled that of AT. It was relatively low in the newborn, increased to a maximum at about 2 weeks and then declined to adult values after another 2 weeks. The serum seromucoid concentration was low in the new born rats and then increased gradually with development to adult levels at about 50 days. The hepatic concentrations of UDP-GlcNAC and CMP-NANA both were relatively high at 24 hr after birth compared to values observed at 50 days. The developmental pattern observed for both over this time period was quite similar and was almost the reciprocal to that for the seromucoid fraction. After 50 days the concentration of UDP-GlcNAc remained constant at adult levels as did that of the seromucoid fraction, but the concentration of CMP-NANA showed a marked increase. It is suggested that the hepatic concentrations of these amino-sugar nucleotides during development primarily reflect the demand for the hepatic synthesis and secretion of plasma glycoproteins as reflected in the seromucoid fraction.", "PMID": 967894}
{"id": "pubmed23n0032_7172", "title": "The sulfhydryl group of monellin: its chemical reactivity and importance to the sweet taste.", "content": "The presence of a single cysteine in the sweet-tasting protein monellin was confirmed by titrations with p-hydroxymercuribenzoate (PHMB) and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). The sulfhydryl group in native monellin reacts very slowly with each of these reagents, indicating that the sulfhydryl is relatively inaccessible. In the presence of either 6 M guanidine-HCl, 8 M urea, or 1% sodium dodecyl sulfate, the rate of reaction of the sulfhydryl group with titrant is dramatically increased. Under a variety of conditions, the presence of 1 mole of sulfhydryl per mole of protein (of molecular weight 10,700) was found. Reaction of the sulfhydryl by titration with PHMB or DTNB leads to loss of sweetness. The free sulfhydryl is also lost by carboxymethylation of monellin in the presence of guanidine-HCl, yielding a protein that is not sweet. Exposure to air in the presence of denaturant leads to a decrease in the sweetness of monellin. Sweetness of the PHMB-reacted monellin can be recovered upon treatment of the protein with mercaptoethanol, and the partial loss of sweetness that occurs with air exposure is lessened in the presence of mercaptoethanol. It is postulated that alteration of the single sulfhydryl group of monellin leads to a change in the tertiary structure of the protein and hence its sweet taste.", "contents": "The sulfhydryl group of monellin: its chemical reactivity and importance to the sweet taste. The presence of a single cysteine in the sweet-tasting protein monellin was confirmed by titrations with p-hydroxymercuribenzoate (PHMB) and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). The sulfhydryl group in native monellin reacts very slowly with each of these reagents, indicating that the sulfhydryl is relatively inaccessible. In the presence of either 6 M guanidine-HCl, 8 M urea, or 1% sodium dodecyl sulfate, the rate of reaction of the sulfhydryl group with titrant is dramatically increased. Under a variety of conditions, the presence of 1 mole of sulfhydryl per mole of protein (of molecular weight 10,700) was found. Reaction of the sulfhydryl by titration with PHMB or DTNB leads to loss of sweetness. The free sulfhydryl is also lost by carboxymethylation of monellin in the presence of guanidine-HCl, yielding a protein that is not sweet. Exposure to air in the presence of denaturant leads to a decrease in the sweetness of monellin. Sweetness of the PHMB-reacted monellin can be recovered upon treatment of the protein with mercaptoethanol, and the partial loss of sweetness that occurs with air exposure is lessened in the presence of mercaptoethanol. It is postulated that alteration of the single sulfhydryl group of monellin leads to a change in the tertiary structure of the protein and hence its sweet taste.", "PMID": 967895}
{"id": "pubmed23n0032_7173", "title": "Effect of tryptophan and its metabolites on gastric emptying of liquid meals in dogs.", "content": "The effects of tryptophan and its metabolites were studied to determine whether the known inhibiting effect of tryptophan on emptying was due to local or systemic effects or due to tryptophan metabolites. In five dogs with chronic gastric fistulas, instillation of 300 ml of DL-kynurenine (5mM), 5-hydroxytryptophan (mM), or 5-hydroxytryptamine (20 mM) into the gut did not slow gastric emptying. Furthermore, iv infusion of L-tryptophan (5, 20, 50 mM), DL-kynurenine (2, 5, 10 mM), 5-hydroxytryptamine (2mM, 10 mM), and 3-indolepyruvic acid (2, 5, 10 mM) also did not slow gastric emptying. These studies indicate that tryptophan slows gastric emptying by exciting a receptor in the gut and not by a direct effect on the stomach or brain or via its major metabolites.", "contents": "Effect of tryptophan and its metabolites on gastric emptying of liquid meals in dogs. The effects of tryptophan and its metabolites were studied to determine whether the known inhibiting effect of tryptophan on emptying was due to local or systemic effects or due to tryptophan metabolites. In five dogs with chronic gastric fistulas, instillation of 300 ml of DL-kynurenine (5mM), 5-hydroxytryptophan (mM), or 5-hydroxytryptamine (20 mM) into the gut did not slow gastric emptying. Furthermore, iv infusion of L-tryptophan (5, 20, 50 mM), DL-kynurenine (2, 5, 10 mM), 5-hydroxytryptamine (2mM, 10 mM), and 3-indolepyruvic acid (2, 5, 10 mM) also did not slow gastric emptying. These studies indicate that tryptophan slows gastric emptying by exciting a receptor in the gut and not by a direct effect on the stomach or brain or via its major metabolites.", "PMID": 967897}
{"id": "pubmed23n0032_7174", "title": "Contribution of luminal concentration of nutrients and osmolality to postprandial intestinal hyperemia in dogs.", "content": "Intestinal blood flow is increased during digestion. This study assesses if the concentration of nutrients and/or osmolality of chyme in the intestinal lumen are factors determining the hyperemia. Six digested food mixtures containing different concentrations of nutrients and/or having different osmolalities were placed into the jejunal lumen, and their effects on local venous outflow compared. The 100% (999 mOsm/kg), 33% (291 mOsm/kg), and 20% (183 mOsm/kg) food mixtures all increased flow, but the 10% food mixture (94 mOsm/kg) did not. The hyperemic effect of 33 and 20% food was similar, but 100% food produced a greater increase in flow than did 33 or 20% food. Luminal placement of a 30% solution of a nonabsorbable substance polyethylene glycol (1000 mOsm/kg) did not alter flow. Also, the vascular effects of 20 or 10% food mixtures were not altered when these mixtures were made isotonic by the addition of NaCl. These studies indicate that lumen osmolality, within a range of 180 to 1000 mOsm/kg, is not a significant factor contributing to the local hyperemia occurring when nutrients are in the gut lumen. However, the concentration of nutrients in the lumen is a factor determining the local hyperemia.", "contents": "Contribution of luminal concentration of nutrients and osmolality to postprandial intestinal hyperemia in dogs. Intestinal blood flow is increased during digestion. This study assesses if the concentration of nutrients and/or osmolality of chyme in the intestinal lumen are factors determining the hyperemia. Six digested food mixtures containing different concentrations of nutrients and/or having different osmolalities were placed into the jejunal lumen, and their effects on local venous outflow compared. The 100% (999 mOsm/kg), 33% (291 mOsm/kg), and 20% (183 mOsm/kg) food mixtures all increased flow, but the 10% food mixture (94 mOsm/kg) did not. The hyperemic effect of 33 and 20% food was similar, but 100% food produced a greater increase in flow than did 33 or 20% food. Luminal placement of a 30% solution of a nonabsorbable substance polyethylene glycol (1000 mOsm/kg) did not alter flow. Also, the vascular effects of 20 or 10% food mixtures were not altered when these mixtures were made isotonic by the addition of NaCl. These studies indicate that lumen osmolality, within a range of 180 to 1000 mOsm/kg, is not a significant factor contributing to the local hyperemia occurring when nutrients are in the gut lumen. However, the concentration of nutrients in the lumen is a factor determining the local hyperemia.", "PMID": 967898}
{"id": "pubmed23n0032_7175", "title": "The contribution of the distal gastrointestinal tract to glucagon-like immunoreactivity secretion in the rat.", "content": "Radioimmunoassay of acid ethanol extracts of the rat intestinal tract showed the presence of glucagon-like immunoreactivity (GLI) from the duodenum to the colon with maximal concentrations in the ileum and colon. Twenty percent glucose instilled in the duodenum at a dose of 2g/kg body weight stimulated a twofold increase in peripheral plasma GLI concentration. When the instilled glucose load was restricted to only the duodenum and jejunum, or to only the ileum and colon, the increase in peripheral plasma GLI was approximately half that seen when the entire gut was exposed to the glucose. It is concluded that the distal gut as well as the proximal gut releases GLI in the rat.", "contents": "The contribution of the distal gastrointestinal tract to glucagon-like immunoreactivity secretion in the rat. Radioimmunoassay of acid ethanol extracts of the rat intestinal tract showed the presence of glucagon-like immunoreactivity (GLI) from the duodenum to the colon with maximal concentrations in the ileum and colon. Twenty percent glucose instilled in the duodenum at a dose of 2g/kg body weight stimulated a twofold increase in peripheral plasma GLI concentration. When the instilled glucose load was restricted to only the duodenum and jejunum, or to only the ileum and colon, the increase in peripheral plasma GLI was approximately half that seen when the entire gut was exposed to the glucose. It is concluded that the distal gut as well as the proximal gut releases GLI in the rat.", "PMID": 967899}
{"id": "pubmed23n0032_7176", "title": "A study of taste and smell of heavy water (99.8%) in rats.", "content": "Restricted to heavy water (99.8%), rats drank it freely for the first day, then they drank progressively less and died within 14 days. When given a choice between distilled water and heavy water (99.8%) rats avoided heavy water partly by virtue of some deleterious effects of heavy water and partly by virtue of a faint smell of heavy water with which the untoward effects could be associated. Rats did not taste heavy water. Whether a higher viscosity of heavy water plays any part could not be determined. Apparently the low intensity of any untoward effects of heavy water and the faintness of its smell could explain the erratic variability of the reactions of rats to heavy water that put it in a different category from other substances tested so far.", "contents": "A study of taste and smell of heavy water (99.8%) in rats. Restricted to heavy water (99.8%), rats drank it freely for the first day, then they drank progressively less and died within 14 days. When given a choice between distilled water and heavy water (99.8%) rats avoided heavy water partly by virtue of some deleterious effects of heavy water and partly by virtue of a faint smell of heavy water with which the untoward effects could be associated. Rats did not taste heavy water. Whether a higher viscosity of heavy water plays any part could not be determined. Apparently the low intensity of any untoward effects of heavy water and the faintness of its smell could explain the erratic variability of the reactions of rats to heavy water that put it in a different category from other substances tested so far.", "PMID": 967900}
{"id": "pubmed23n0032_7177", "title": "Palmitate incorporation in the lungs of dogs with granulomatous disease.", "content": "Moderate hypoxia did not influence the pulmonary incorporation of an intravenous dose of [1-14C]palmitate either in dogs with experimentally produced granulomatous disease or in normal controls. The lung weight in the diseased animals, was, on the average, double that of the controls. There was a proportionate increase in uptake of the radioactive label at 1 hr after infusion in the diseased lungs, hence the specific activity of labeled palmitate (counts per minute per gram of phospholipid) was no different in the two groups. Moreover, half the radioactivity of the phospholipids was recovered in palmitate separated from the phosphatidyl choline fraction in both diseased and normal lungs. Anatomic studies demonstrated increased numbers of Type II pneumocytes lining all alveolar air spaces in the diseased lung. Autoradiographic studies indicated the presence of labeled palmitate in the Type II cells, but not in the inflammatory cells of the granulomata. We conclude that the increased palmitate uptake in this disease is accounted for by the metabolic activity of the Type II pneumocytes.", "contents": "Palmitate incorporation in the lungs of dogs with granulomatous disease. Moderate hypoxia did not influence the pulmonary incorporation of an intravenous dose of [1-14C]palmitate either in dogs with experimentally produced granulomatous disease or in normal controls. The lung weight in the diseased animals, was, on the average, double that of the controls. There was a proportionate increase in uptake of the radioactive label at 1 hr after infusion in the diseased lungs, hence the specific activity of labeled palmitate (counts per minute per gram of phospholipid) was no different in the two groups. Moreover, half the radioactivity of the phospholipids was recovered in palmitate separated from the phosphatidyl choline fraction in both diseased and normal lungs. Anatomic studies demonstrated increased numbers of Type II pneumocytes lining all alveolar air spaces in the diseased lung. Autoradiographic studies indicated the presence of labeled palmitate in the Type II cells, but not in the inflammatory cells of the granulomata. We conclude that the increased palmitate uptake in this disease is accounted for by the metabolic activity of the Type II pneumocytes.", "PMID": 967901}
{"id": "pubmed23n0032_7178", "title": "Energy-selective reconstructions in X-ray computerized tomography.", "content": "All X-ray computerized tomography systems that are available or proposed base their reconstructions on measurements that integrate over energy. X-ray tubes produce a broad spectrum of photon energies and a great deal of information can be derived by measuring changes in the transmitted spectrum. We show that for any material, complete energy spectral information may be summarized by a few constants which are independent of energy. A technique is presented which uses simple, low-resolution, energy spectrum measurements and conventional computerized tomography techniques to calculate these constants at every point within a cross-section of an object. For comparable accuracy, patient dose is shown to be approximately the same as that produced by conventional systems. Possible uses of energy spectral information for diagnosis are presented.", "contents": "Energy-selective reconstructions in X-ray computerized tomography. All X-ray computerized tomography systems that are available or proposed base their reconstructions on measurements that integrate over energy. X-ray tubes produce a broad spectrum of photon energies and a great deal of information can be derived by measuring changes in the transmitted spectrum. We show that for any material, complete energy spectral information may be summarized by a few constants which are independent of energy. A technique is presented which uses simple, low-resolution, energy spectrum measurements and conventional computerized tomography techniques to calculate these constants at every point within a cross-section of an object. For comparable accuracy, patient dose is shown to be approximately the same as that produced by conventional systems. Possible uses of energy spectral information for diagnosis are presented.", "PMID": 967922}
{"id": "pubmed23n0032_7179", "title": "A technique for measuring the variation of photon spectrum from X-ray generators over the mains voltage cycle.", "content": "The variation in current and accelerating voltage across an X-ray tube, that occurs over the mains voltage waveform cycle, produces changes in photon flux and spectrum shape. A knowledge of these changes is required to provide an understanding of the parameters affecting X-ray output. Variation in the photon flux will cause distortion of a measured 'mean' spectrum if the dead time of the spectrometry system employed varies over the waveform cycle. A system has been developed that enables the spectrometer to be synchronized to the mains voltage cycle so that variations in photon flux and the instantaneous spectra at selected parts of this cycle can be measured. The variation in photon flux is dependent upon the type of power supplies (both high and low tension). the tube current and the degree of filtration employed. Examples are given of the ripple of photon output due to the voltage and current ripple for a nominally constant potential generator and a half-wave rectified type. The way in which they interact to produce the measured variation is shown.", "contents": "A technique for measuring the variation of photon spectrum from X-ray generators over the mains voltage cycle. The variation in current and accelerating voltage across an X-ray tube, that occurs over the mains voltage waveform cycle, produces changes in photon flux and spectrum shape. A knowledge of these changes is required to provide an understanding of the parameters affecting X-ray output. Variation in the photon flux will cause distortion of a measured 'mean' spectrum if the dead time of the spectrometry system employed varies over the waveform cycle. A system has been developed that enables the spectrometer to be synchronized to the mains voltage cycle so that variations in photon flux and the instantaneous spectra at selected parts of this cycle can be measured. The variation in photon flux is dependent upon the type of power supplies (both high and low tension). the tube current and the degree of filtration employed. Examples are given of the ripple of photon output due to the voltage and current ripple for a nominally constant potential generator and a half-wave rectified type. The way in which they interact to produce the measured variation is shown.", "PMID": 967923}
{"id": "pubmed23n0032_7180", "title": "Saturation corrections for plane-parallel ionization chambers.", "content": "Experiments described in the literature lead to different formulae for saturation corrections in ionization chambers. To elucidate the differences, saturation curves of an extrapolation chamber irradiated with beta particles from 90Sr+90Y sources have been studied experimentally. The results could be described by one formula which was a combination of known formulae for charge collection losses due to volume recombination, initial recombination and diffusion.", "contents": "Saturation corrections for plane-parallel ionization chambers. Experiments described in the literature lead to different formulae for saturation corrections in ionization chambers. To elucidate the differences, saturation curves of an extrapolation chamber irradiated with beta particles from 90Sr+90Y sources have been studied experimentally. The results could be described by one formula which was a combination of known formulae for charge collection losses due to volume recombination, initial recombination and diffusion.", "PMID": 967924}
{"id": "pubmed23n0032_7181", "title": "Bone density measured by photon scattering. I. A system for clinical use.", "content": "A system for the routine measurement of the density of the os calcis is described. Measurements are made of the number of photons scattered by the bone from a 153Sm photon beam. The first results from human subjects are presented.", "contents": "Bone density measured by photon scattering. I. A system for clinical use. A system for the routine measurement of the density of the os calcis is described. Measurements are made of the number of photons scattered by the bone from a 153Sm photon beam. The first results from human subjects are presented.", "PMID": 967925}
{"id": "pubmed23n0032_7182", "title": "Bone density measured by photon scattering. II. Inherent sources of error.", "content": "The origins of inherent sources of error in results of measurements of bone density by a photon scattering technique are described and their effects are predicted theoretically. The predictions are confirmed experimentally. The prime source of error is multiple scattering. A procedure has been developed to correct the observed densities for effects of multiple scattering.", "contents": "Bone density measured by photon scattering. II. Inherent sources of error. The origins of inherent sources of error in results of measurements of bone density by a photon scattering technique are described and their effects are predicted theoretically. The predictions are confirmed experimentally. The prime source of error is multiple scattering. A procedure has been developed to correct the observed densities for effects of multiple scattering.", "PMID": 967926}
{"id": "pubmed23n0032_7183", "title": "A new technique for radiotherapy planning using quadratic programming.", "content": "Previous attempts at optimization of radiotherapy planning are described and criticized, and consideration of these attempts has resulted in the development of a new technique using quadratic programming. Uniformity of tumour dose is selected as the most important feature of any plan, and this is achieved by minimizing the variance of the dose to preselected points within the tumour. The dose to vulnerable regions can be constrained not to exceed a given percentage of the mean tumour dose. Optimization of field weight and field type is possible. The operation of the system is described and some typical results are given.", "contents": "A new technique for radiotherapy planning using quadratic programming. Previous attempts at optimization of radiotherapy planning are described and criticized, and consideration of these attempts has resulted in the development of a new technique using quadratic programming. Uniformity of tumour dose is selected as the most important feature of any plan, and this is achieved by minimizing the variance of the dose to preselected points within the tumour. The dose to vulnerable regions can be constrained not to exceed a given percentage of the mean tumour dose. Optimization of field weight and field type is possible. The operation of the system is described and some typical results are given.", "PMID": 967927}
{"id": "pubmed23n0032_7184", "title": "Digital computer processing of brain scans using principal components.", "content": "Most of the scan processing methods used to date are unable to distinguish between normal and abnormal features. This paper describes a method of constructing a particular orthogonal transform from a set of normal images, the method of principal components, which may be used selectively to filter out normal features, leaving behind abnormal features. The method has been applied to brain scans and has been implemented on a small digital computer system with magnetic disc.", "contents": "Digital computer processing of brain scans using principal components. Most of the scan processing methods used to date are unable to distinguish between normal and abnormal features. This paper describes a method of constructing a particular orthogonal transform from a set of normal images, the method of principal components, which may be used selectively to filter out normal features, leaving behind abnormal features. The method has been applied to brain scans and has been implemented on a small digital computer system with magnetic disc.", "PMID": 967928}
{"id": "pubmed23n0032_7185", "title": "Dosimetry in single lung cells by means of microautoradiographic activity measurements.", "content": "After inhalation of compounds containing promethium-147 in the lungs of mice most of the activity is deposited in the form of local concentrations (hotspots). By means of a special quantitative microautoradiographic method using stripping film ORWO K 105, measurements of the activity of single hotspots of about 10(-14) Ci are possible. A microphotometer with a variable measuring diaphragm is used for the determination of the density profile of the autoradiographic image in order to get hotspot depth within the biological specimen. To determine hotspot activity it is necessary to calibrate the film with a Pm-147 plane source. The systematic and random errors of the method are discussed in detail, giving a total error of +/- 21% (SD) for one hotspot activity measurement. A few examples of biological results obtained by the method are given. Simple models are used to calculate doses absorbed in macrophage and alveolar cell nuclei from the measured activities.", "contents": "Dosimetry in single lung cells by means of microautoradiographic activity measurements. After inhalation of compounds containing promethium-147 in the lungs of mice most of the activity is deposited in the form of local concentrations (hotspots). By means of a special quantitative microautoradiographic method using stripping film ORWO K 105, measurements of the activity of single hotspots of about 10(-14) Ci are possible. A microphotometer with a variable measuring diaphragm is used for the determination of the density profile of the autoradiographic image in order to get hotspot depth within the biological specimen. To determine hotspot activity it is necessary to calibrate the film with a Pm-147 plane source. The systematic and random errors of the method are discussed in detail, giving a total error of +/- 21% (SD) for one hotspot activity measurement. A few examples of biological results obtained by the method are given. Simple models are used to calculate doses absorbed in macrophage and alveolar cell nuclei from the measured activities.", "PMID": 967929}
{"id": "pubmed23n0032_7186", "title": "The response of cells in culture to fractionated radiation: a theoretical approach.", "content": "A mathematical model has been constructed to simulate the response of cells in vitro to fractionated doses of radiation. The model is capable of describing most of the radiobiological functions that are commonly studied. Input data are measurable cell properties. The details of the model and methods of acquiring the input data are outlined and a comparison with experimental observations on two radiobiological functions, survival curves and recovery curves are discussed in some detail.", "contents": "The response of cells in culture to fractionated radiation: a theoretical approach. A mathematical model has been constructed to simulate the response of cells in vitro to fractionated doses of radiation. The model is capable of describing most of the radiobiological functions that are commonly studied. Input data are measurable cell properties. The details of the model and methods of acquiring the input data are outlined and a comparison with experimental observations on two radiobiological functions, survival curves and recovery curves are discussed in some detail.", "PMID": 967930}
{"id": "pubmed23n0032_7187", "title": "Program for cardiac patients: stress testing and training.", "content": "Dynamic exercise stress testing had become a widely accepted technique in the diagnosis of cardiac patients. The results of standardized exercise testing may be used to evaluate a patient's functional capacity and to derive an exercise prescription to assist him in improving his state of cardiovascular fitness. Because the physical therapist has special training and skill in exercise-related techniques, a stress testing laboratory and an exercise-based cardiac rehabilitation program may be appropriately established in a hospital's physical therapy department. Results are presented of a diagnostic exercise testing and an exercise-based cardiac rehabilitation program organized and supervised by physical therapists in a 300-bed private hospital.", "contents": "Program for cardiac patients: stress testing and training. Dynamic exercise stress testing had become a widely accepted technique in the diagnosis of cardiac patients. The results of standardized exercise testing may be used to evaluate a patient's functional capacity and to derive an exercise prescription to assist him in improving his state of cardiovascular fitness. Because the physical therapist has special training and skill in exercise-related techniques, a stress testing laboratory and an exercise-based cardiac rehabilitation program may be appropriately established in a hospital's physical therapy department. Results are presented of a diagnostic exercise testing and an exercise-based cardiac rehabilitation program organized and supervised by physical therapists in a 300-bed private hospital.", "PMID": 967937}
{"id": "pubmed23n0032_7188", "title": "A method of developing and evaluating a clinical performance program for physical therapy interns.", "content": "A method used by interns and supervisors in developing and evaluating a clinical performance program during a 6-week internship in the Division of Physical Therapy at the University of Michigan Medical Center is presented. This method required a statement of educational resources available, establishment of criteria for judging acceptable performance, statement by the intern of his educational objectives, negotiation of a written contract, and maintenance of a log. The 32 participants thought that this was a rational and acceptable program and stated that they would, if given a choice, elect to follow the same procedure again.", "contents": "A method of developing and evaluating a clinical performance program for physical therapy interns. A method used by interns and supervisors in developing and evaluating a clinical performance program during a 6-week internship in the Division of Physical Therapy at the University of Michigan Medical Center is presented. This method required a statement of educational resources available, establishment of criteria for judging acceptable performance, statement by the intern of his educational objectives, negotiation of a written contract, and maintenance of a log. The 32 participants thought that this was a rational and acceptable program and stated that they would, if given a choice, elect to follow the same procedure again.", "PMID": 967938}
{"id": "pubmed23n0032_7189", "title": "[The motormetric Rostock-Oseretzky scale].", "content": "A new motometric scale with 24 tasks was designed taking into account Oseretzky tasks. It was standardized with 325 normal school children ranging in age from 5 to 11 years. The normal presentation in form of C scales and MQ scales, resp., allows a qualitative evaluation of the motor development irrespective of the age of the child. Furthermore, the procedure allows an improvement in the successful diagnosis of cerebral lesions in early infancy; this was verified by an examination of 92 children with proved brain lesions or high risk births. Details are given on the reliability, validity with reference to criteria, and diagnostic value of the scale. The procedure is economic as far as the necessary time is concerned and therefore it can also be applied in the treatment of out-patients.", "contents": "[The motormetric Rostock-Oseretzky scale]. A new motometric scale with 24 tasks was designed taking into account Oseretzky tasks. It was standardized with 325 normal school children ranging in age from 5 to 11 years. The normal presentation in form of C scales and MQ scales, resp., allows a qualitative evaluation of the motor development irrespective of the age of the child. Furthermore, the procedure allows an improvement in the successful diagnosis of cerebral lesions in early infancy; this was verified by an examination of 92 children with proved brain lesions or high risk births. Details are given on the reliability, validity with reference to criteria, and diagnostic value of the scale. The procedure is economic as far as the necessary time is concerned and therefore it can also be applied in the treatment of out-patients.", "PMID": 967945}
{"id": "pubmed23n0032_7190", "title": "[The attitude toward learning and favorite subjects in students with reading and spelling disability].", "content": "The hypothesis of a general dislike of school and learning aversion in children with reading and spelling disability could not be confirmed. As far as the attitude to the subjects and to the teachers, particularly to the teachers of German, is concerned no deviation from the norm generally existed. The inclination towards lingual subjects is lower than in pupils attending polytechnic high schools. The contrast between frequent behavioural disorders in school on the one hand, and the general positive attitude towards school is interpreted as a appetency-aversion conflict. The dominance of appetency is caused by the favourable social and pedagogic conditions in socialist society and is regarded as a prerequisite of successful therapy.", "contents": "[The attitude toward learning and favorite subjects in students with reading and spelling disability]. The hypothesis of a general dislike of school and learning aversion in children with reading and spelling disability could not be confirmed. As far as the attitude to the subjects and to the teachers, particularly to the teachers of German, is concerned no deviation from the norm generally existed. The inclination towards lingual subjects is lower than in pupils attending polytechnic high schools. The contrast between frequent behavioural disorders in school on the one hand, and the general positive attitude towards school is interpreted as a appetency-aversion conflict. The dominance of appetency is caused by the favourable social and pedagogic conditions in socialist society and is regarded as a prerequisite of successful therapy.", "PMID": 967946}
{"id": "pubmed23n0032_7191", "title": "[Secondary symptoms of reading and spelling disability].", "content": "The hypothesis that the secondary symptoms of legasthenia can be derived in a linear and causal way from the primary symptoms is investigated. This study is based on data from the patients' history, diagnosis, therapy, and catamnesis. It is shown, that behavioural and adaptability disturbances are for the major part of prelegasthenic character but may become more pronounced by the manifestation of the primary symptoms. The parallel course of primary and so-called secondary symptoms with different times of manifestation is discussed as a conceptional model. Furthermore, the much less fully authenticated conceptional model based on findings in a childrens' neuropsychiatric clinic, which claims the existence of genuine secondary symptoms in a causal relationship to the primary symptoms, is not put into dispute.", "contents": "[Secondary symptoms of reading and spelling disability]. The hypothesis that the secondary symptoms of legasthenia can be derived in a linear and causal way from the primary symptoms is investigated. This study is based on data from the patients' history, diagnosis, therapy, and catamnesis. It is shown, that behavioural and adaptability disturbances are for the major part of prelegasthenic character but may become more pronounced by the manifestation of the primary symptoms. The parallel course of primary and so-called secondary symptoms with different times of manifestation is discussed as a conceptional model. Furthermore, the much less fully authenticated conceptional model based on findings in a childrens' neuropsychiatric clinic, which claims the existence of genuine secondary symptoms in a causal relationship to the primary symptoms, is not put into dispute.", "PMID": 967947}
{"id": "pubmed23n0032_7192", "title": "[Excretion of 3,4-dimethoxy phenylethylamine in the urine of schizophrenics].", "content": "According to the hypothesis of Osmond, Harley-Mason and Smythies on abnormal methylation products in the catecholamine metabolism in schizophrenics, the excretion of 3,4-dimethoxyphenylethylamine (3,4-DMPEA) in the urine was examined in 80 patients with endogenous psychoses and in 20 normal persons. In 38% of the patients 3,4-DMPEA was found, whereas no 3,4-DMPEA was found in normal persons. When the biochemical findings obtained are correlated to the corresponding diagnoses the highest incidence of positive findings was observed in paranoid-hallucinatory schizophrenics with 71% in males and 75% in females. The signification of controls during the course of the experiments is emphasized.", "contents": "[Excretion of 3,4-dimethoxy phenylethylamine in the urine of schizophrenics]. According to the hypothesis of Osmond, Harley-Mason and Smythies on abnormal methylation products in the catecholamine metabolism in schizophrenics, the excretion of 3,4-dimethoxyphenylethylamine (3,4-DMPEA) in the urine was examined in 80 patients with endogenous psychoses and in 20 normal persons. In 38% of the patients 3,4-DMPEA was found, whereas no 3,4-DMPEA was found in normal persons. When the biochemical findings obtained are correlated to the corresponding diagnoses the highest incidence of positive findings was observed in paranoid-hallucinatory schizophrenics with 71% in males and 75% in females. The signification of controls during the course of the experiments is emphasized.", "PMID": 967948}
{"id": "pubmed23n0032_7193", "title": "[Correlation between reflex behavior and EEG in a brain-stem tumor].", "content": "In a female patient with a tumour of the brain stem the e.e.g. showed signs of increasing general functional disorders and additional transient high voltage monographic waves with bilateral frontal and central accentuation, which we regarded as brain stem disturbances. At the same time the authors observed a characteristic inhibition of the amplitude of brain reflexes as well as high phases of a polyphasic stimulation--response potential with stimulation of the tibial nerve. Beside these inhibition phenomena, signs of disinhibition were verified (nociceptive, trigemino-trigeminal reflex, polyphasic disinhibited brain reflex?). The photostimulation was without influence on the sensitivity of motoneurons.", "contents": "[Correlation between reflex behavior and EEG in a brain-stem tumor]. In a female patient with a tumour of the brain stem the e.e.g. showed signs of increasing general functional disorders and additional transient high voltage monographic waves with bilateral frontal and central accentuation, which we regarded as brain stem disturbances. At the same time the authors observed a characteristic inhibition of the amplitude of brain reflexes as well as high phases of a polyphasic stimulation--response potential with stimulation of the tibial nerve. Beside these inhibition phenomena, signs of disinhibition were verified (nociceptive, trigemino-trigeminal reflex, polyphasic disinhibited brain reflex?). The photostimulation was without influence on the sensitivity of motoneurons.", "PMID": 967949}
{"id": "pubmed23n0032_7194", "title": "An analysis of career enquiries made to a school of radiography over a five-year period.", "content": "A follow-up enquiry form is described and results provided from a survey over a five-year period. The information gained has enabled a further and we hope more searching questionnaire to be designed. At the time of producing the pro-forma in 1969, career and salary prospects in radiography were not as good as at present. The Halsbury Report in May 1974 was a great advantage to the paramedical professions as a whole. It will be interesting to see how recruitment and job availability proceed over the ensuing five years.", "contents": "An analysis of career enquiries made to a school of radiography over a five-year period. A follow-up enquiry form is described and results provided from a survey over a five-year period. The information gained has enabled a further and we hope more searching questionnaire to be designed. At the time of producing the pro-forma in 1969, career and salary prospects in radiography were not as good as at present. The Halsbury Report in May 1974 was a great advantage to the paramedical professions as a whole. It will be interesting to see how recruitment and job availability proceed over the ensuing five years.", "PMID": 968022}
{"id": "pubmed23n0032_7195", "title": "The value of lead shields in reducing radiation dosage to the eye during neuroradiological procedures.", "content": "Home-made protective eyeshields are described, for use during certain types of skull radiography. Measurements made during the examination of patients and with a phantom skull indicate that a ten-fold reduction in radiation dose may be achieved through their use during certain diagnostic procedures.", "contents": "The value of lead shields in reducing radiation dosage to the eye during neuroradiological procedures. Home-made protective eyeshields are described, for use during certain types of skull radiography. Measurements made during the examination of patients and with a phantom skull indicate that a ten-fold reduction in radiation dose may be achieved through their use during certain diagnostic procedures.", "PMID": 968025}
{"id": "pubmed23n0032_7196", "title": "A method of measuring the angle of tomographic movement.", "content": "A technique is described for measuring the angle of two tomographic movements. It requires only simple equipment, most of which is normally available in X-ray departments. The calculation involved is quite simple and the result obtained is accurate even at small angles.", "contents": "A method of measuring the angle of tomographic movement. A technique is described for measuring the angle of two tomographic movements. It requires only simple equipment, most of which is normally available in X-ray departments. The calculation involved is quite simple and the result obtained is accurate even at small angles.", "PMID": 968031}
{"id": "pubmed23n0032_7197", "title": "Double-contrast arthrography in meniscal lesions and patellar chondropathy.", "content": "The technique of double-contrast arthrography is discussed in this paper. The serial exposures included a special exposure of the retropatellar region in order to detect patellar chondropathy. Various types of meniscal rupture and degeneration are discussed with special reference to combinations of these lesions and to lesions of both menisci in the same knee joint. The aetiology and radiological features of patellar chondropathy are discussed in detail. Patellar chondropathy was diagnosed in 19% of patients without meniscal lesions, 23% of patients with medial and 35% of those with lateral meniscal lesions, as well as in 73% of post-meniscectomy cases. Comparison of arthrographic with arthroscopic results revealed a positive correlation in 74% of cases.", "contents": "Double-contrast arthrography in meniscal lesions and patellar chondropathy. The technique of double-contrast arthrography is discussed in this paper. The serial exposures included a special exposure of the retropatellar region in order to detect patellar chondropathy. Various types of meniscal rupture and degeneration are discussed with special reference to combinations of these lesions and to lesions of both menisci in the same knee joint. The aetiology and radiological features of patellar chondropathy are discussed in detail. Patellar chondropathy was diagnosed in 19% of patients without meniscal lesions, 23% of patients with medial and 35% of those with lateral meniscal lesions, as well as in 73% of post-meniscectomy cases. Comparison of arthrographic with arthroscopic results revealed a positive correlation in 74% of cases.", "PMID": 968032}
{"id": "pubmed23n0032_7198", "title": "Echoscopic and echographic differential diagnosis of pancreatic lesions.", "content": "Combined echoscopic and echographic examination enables accurate display of the pancreatic region. Furthermore, this method yields reliable results in demonstrating pathological changes of the pancreas caused by acute pancreatitis, acute necrotising pancreatitis, pancreatic pseudocyst and neoplasm of the pancreas. The characteristic ultrasonographic findings in these diseases are presented and the differential diagnosis is briefly discussed.", "contents": "Echoscopic and echographic differential diagnosis of pancreatic lesions. Combined echoscopic and echographic examination enables accurate display of the pancreatic region. Furthermore, this method yields reliable results in demonstrating pathological changes of the pancreas caused by acute pancreatitis, acute necrotising pancreatitis, pancreatic pseudocyst and neoplasm of the pancreas. The characteristic ultrasonographic findings in these diseases are presented and the differential diagnosis is briefly discussed.", "PMID": 968033}
{"id": "pubmed23n0032_7199", "title": "Serial liver and spleen scanning in patients with malignant lymphomas.", "content": "Spleen and liver represent target organs for involvement in patients with Hodgkin's disease and with non-Hodgkin's lymphomas. Although histological examination remains the most reliable method in diagnosing the presence of disease in these organs, particularly during the early phases, exploratory laparotomy and liver biopsy cannot be repeated, for practical purposes, on a regular basis. As the incidence of splenic and hepatic involvement continues to rise in the years following the initial diagnosis, we have found that liver and spleen scans performed in a serial manner are helpful in establishing the diagnosis during the follow-up period. By comparing the later studies to the earlier ones, interval changes as related to the size, contour, homogeneity and to the presence or absence of filling defects, become apparent. These changes, should they occur, are related, in our experience to the underlying lymphoma. Conversely, through serial scanning, improvement under treatment of a previously involved organ, is well demonstrated by this noninvasive technique. The limitations imposed by the resolving power of the imaging system must be taken into consideration when interpreting the results.", "contents": "Serial liver and spleen scanning in patients with malignant lymphomas. Spleen and liver represent target organs for involvement in patients with Hodgkin's disease and with non-Hodgkin's lymphomas. Although histological examination remains the most reliable method in diagnosing the presence of disease in these organs, particularly during the early phases, exploratory laparotomy and liver biopsy cannot be repeated, for practical purposes, on a regular basis. As the incidence of splenic and hepatic involvement continues to rise in the years following the initial diagnosis, we have found that liver and spleen scans performed in a serial manner are helpful in establishing the diagnosis during the follow-up period. By comparing the later studies to the earlier ones, interval changes as related to the size, contour, homogeneity and to the presence or absence of filling defects, become apparent. These changes, should they occur, are related, in our experience to the underlying lymphoma. Conversely, through serial scanning, improvement under treatment of a previously involved organ, is well demonstrated by this noninvasive technique. The limitations imposed by the resolving power of the imaging system must be taken into consideration when interpreting the results.", "PMID": 968034}
{"id": "pubmed23n0032_7200", "title": "Frequency of bilateral tumours and primary retroperitoneal localization in tumours of the testicles.", "content": "Report on 4 patients with bilateral testicular tumours (out of a total number of 181 cases) and the problems arising with a second course of irradiation. The question of histogenesis and therapeutic course in 4 patients with a primary retroperitoneal tumour localization are discussed in the second part.", "contents": "Frequency of bilateral tumours and primary retroperitoneal localization in tumours of the testicles. Report on 4 patients with bilateral testicular tumours (out of a total number of 181 cases) and the problems arising with a second course of irradiation. The question of histogenesis and therapeutic course in 4 patients with a primary retroperitoneal tumour localization are discussed in the second part.", "PMID": 968035}
{"id": "pubmed23n0032_7201", "title": "A short course - low dose preoperative irradiation of endometrial carcinoma. Preliminary report.", "content": "This is a preliminary and comparative report of a randomized prospective study in which 14 unselected women with a proven histological diagnosis of endometrial adenocarcinoma were subjected to a course of external irradiation treatment with total 1,500 rad mid-pelvic dose delivered on five consecutive days followed by total abdominal hysterectomy and bilateral salpingo-oophorectomy. Surgery was performed within 1 week after the completion of radiation therapy. At the same time, 15 additional patients with identical histological diagnosis were treated with the conventional external irradiation technique of 5,000 rad delivered in 5 weeks followed by surgery 6 weeks later. The stage indicates no significant differences between these two radiotherapeutic modalities in regard to survival rate, recurrences or complications. The short course-low dose approach markedly reduces the cost and overall treatment time.", "contents": "A short course - low dose preoperative irradiation of endometrial carcinoma. Preliminary report. This is a preliminary and comparative report of a randomized prospective study in which 14 unselected women with a proven histological diagnosis of endometrial adenocarcinoma were subjected to a course of external irradiation treatment with total 1,500 rad mid-pelvic dose delivered on five consecutive days followed by total abdominal hysterectomy and bilateral salpingo-oophorectomy. Surgery was performed within 1 week after the completion of radiation therapy. At the same time, 15 additional patients with identical histological diagnosis were treated with the conventional external irradiation technique of 5,000 rad delivered in 5 weeks followed by surgery 6 weeks later. The stage indicates no significant differences between these two radiotherapeutic modalities in regard to survival rate, recurrences or complications. The short course-low dose approach markedly reduces the cost and overall treatment time.", "PMID": 968036}
{"id": "pubmed23n0032_7202", "title": "[Carcinoids of the small intestine. Retrospective study of the radiological diagnosis of 15 patients (author's transl)].", "content": "The radiological workup of 15 patients with a carcinoid has been evaluated. As 7 of the 15 patients presented for emergency operation, only the remaining 8 had barium studies (7 barium meals and 1 barium enema). Two of the barium meals were even retrospectively, entirely negative. In two others, initially the diagnosis was missed despite radiological signs, which had been overlooked on follow-up films. Fluoroscopic examination of the small bowel would probably have ensured the detection of the lesion. At the time of diagnosis nine tenth of the tumours were already infiltrating and more than half of them metastasizing. The presence of the carcinoid syndrome implies the production of the causative hormones in the liver or the pulmonary circulation. In order to find the primary intestinal carcinoid in a cureable stage- that is, before it has metastasized-, all patients with diarrhoea and repeated bouts of abdominal pain should have a careful follow-up examination of the small bowel. This examination must include fluoroscopies.", "contents": "[Carcinoids of the small intestine. Retrospective study of the radiological diagnosis of 15 patients (author's transl)]. The radiological workup of 15 patients with a carcinoid has been evaluated. As 7 of the 15 patients presented for emergency operation, only the remaining 8 had barium studies (7 barium meals and 1 barium enema). Two of the barium meals were even retrospectively, entirely negative. In two others, initially the diagnosis was missed despite radiological signs, which had been overlooked on follow-up films. Fluoroscopic examination of the small bowel would probably have ensured the detection of the lesion. At the time of diagnosis nine tenth of the tumours were already infiltrating and more than half of them metastasizing. The presence of the carcinoid syndrome implies the production of the causative hormones in the liver or the pulmonary circulation. In order to find the primary intestinal carcinoid in a cureable stage- that is, before it has metastasized-, all patients with diarrhoea and repeated bouts of abdominal pain should have a careful follow-up examination of the small bowel. This examination must include fluoroscopies.", "PMID": 968037}
{"id": "pubmed23n0032_7203", "title": "[An analysis of the plain chest films of 109 children with atrial septal defect. The correlation between different radiological criteria and shunt size (author's transl)].", "content": "Radiograms of the chest (p.a.--and lateral projection) of 109 children with atrial septal defect taken before cardiac catheterisation have been evaluated under standardized principles; correlation between size of the left-to-right shunt and the radiographic changes has been performed. The examiner evaluated at random radiographs of 213 subjects, of whom 104 had no cardiac disease. Besides the determination of several radiographic criteria the examiner had to decide whether the subject in question had cardiac abnormalities or not. There has been a statistically significant correlation between the size of shunt-volume and the amount of radiographic changes as: 1. pulmonary vascular markings, 2. size of the right ventricle in the lateral projection, 3. cardiac configuration in the p.a.-projection, 4. relative cardiac volume, 5. size and shape of the pulmonary artery segment, 6. size of the aortic arch. The highest diagnostic value is represented by pulmonary vascular markings; this is of great importance. Because pulmonary vascular markings can be in any age group evaluated on technically good radiographs. 78% of the atrial septal defects could be detected by radiographic criteria. In 5% of the children with no cardiac disease a vitium cordis had been diagnosed erroneously.", "contents": "[An analysis of the plain chest films of 109 children with atrial septal defect. The correlation between different radiological criteria and shunt size (author's transl)]. Radiograms of the chest (p.a.--and lateral projection) of 109 children with atrial septal defect taken before cardiac catheterisation have been evaluated under standardized principles; correlation between size of the left-to-right shunt and the radiographic changes has been performed. The examiner evaluated at random radiographs of 213 subjects, of whom 104 had no cardiac disease. Besides the determination of several radiographic criteria the examiner had to decide whether the subject in question had cardiac abnormalities or not. There has been a statistically significant correlation between the size of shunt-volume and the amount of radiographic changes as: 1. pulmonary vascular markings, 2. size of the right ventricle in the lateral projection, 3. cardiac configuration in the p.a.-projection, 4. relative cardiac volume, 5. size and shape of the pulmonary artery segment, 6. size of the aortic arch. The highest diagnostic value is represented by pulmonary vascular markings; this is of great importance. Because pulmonary vascular markings can be in any age group evaluated on technically good radiographs. 78% of the atrial septal defects could be detected by radiographic criteria. In 5% of the children with no cardiac disease a vitium cordis had been diagnosed erroneously.", "PMID": 968041}
{"id": "pubmed23n0032_7204", "title": "[Several causes of respiratory insufficiency in children (author's transl)].", "content": "Differential diagnosis respiratory insufficiency in children is great problem for pediatricians and a radiologists. The mortality rate for respiratory insufficiency especially in the newborn may be as high as 50 per cent. The etiology of respiratory insufficiency may be of different origin. Considering location one can distinguish between pulmonary and extrapulmonary causes, as congenital oesophageal stresia, diaphragmatic hernia, diaphragmatic relaxation, and tumours of the neck, mediastinum and thoracic wall. Roentgen examinations are important to diagnose of extrapulmonary causes for respiratory insufficiency.", "contents": "[Several causes of respiratory insufficiency in children (author's transl)]. Differential diagnosis respiratory insufficiency in children is great problem for pediatricians and a radiologists. The mortality rate for respiratory insufficiency especially in the newborn may be as high as 50 per cent. The etiology of respiratory insufficiency may be of different origin. Considering location one can distinguish between pulmonary and extrapulmonary causes, as congenital oesophageal stresia, diaphragmatic hernia, diaphragmatic relaxation, and tumours of the neck, mediastinum and thoracic wall. Roentgen examinations are important to diagnose of extrapulmonary causes for respiratory insufficiency.", "PMID": 968042}
{"id": "pubmed23n0032_7205", "title": "[Radiographic signs of cor pulmonale (author's transl)].", "content": "The pulmonary artery pressure of 47 patients was compared with radiological criteria of pulmonary hypertension: in rest and exercise. The diameter of the truncus intermediul is the only measurable criterium. A diameter larger than or equal to 18 mm is an indication for latent or manifest cor pulmonale. If the diameter is less than or equal to 15 mm a pulmonary hypertension is improbably. Additional findings (primary pulmonary disease, diameter of the truncus pulmonalis, loss of lung vessel structure in the periphery, marked reduction in vessel caliber--graded 0-3) leads to the diagnosis of manifest cor pulmonale on the basis of routine x-ray of the chest. The differentiation between manifest and latent cor pulmonale ist possible in most cases. An overestimation of the primary lung disease, particularly by fibrosis, may mistead from the correct diagnosis.", "contents": "[Radiographic signs of cor pulmonale (author's transl)]. The pulmonary artery pressure of 47 patients was compared with radiological criteria of pulmonary hypertension: in rest and exercise. The diameter of the truncus intermediul is the only measurable criterium. A diameter larger than or equal to 18 mm is an indication for latent or manifest cor pulmonale. If the diameter is less than or equal to 15 mm a pulmonary hypertension is improbably. Additional findings (primary pulmonary disease, diameter of the truncus pulmonalis, loss of lung vessel structure in the periphery, marked reduction in vessel caliber--graded 0-3) leads to the diagnosis of manifest cor pulmonale on the basis of routine x-ray of the chest. The differentiation between manifest and latent cor pulmonale ist possible in most cases. An overestimation of the primary lung disease, particularly by fibrosis, may mistead from the correct diagnosis.", "PMID": 968043}
{"id": "pubmed23n0032_7206", "title": "Prostaglandin endoperoxide and thromboxane generating systems and their selective inhibition.", "content": "Two enzyme systems and their selective inhibition are described. Microsomes from ram seminal vesicles (RSV) incubated with arachidonic acid at 22 degrees C generated a rabbit aorta contracting substance which, after rapid ether extraction, had characteristics similar to purified standard endoperoxides. Incubation of either purified endoperoxide or the product from RSV and arachidonic acid with horse platelet microsomes (HPM) yielded a more potent rabbit aorta contracting substance characterized as thromboxane A2, with a half life of 35.9 +/- 2.2 s at 37 degrees C after ether extraction. Two inhibitors, indomethacin and benzydamine exhibited selectivity for the two enzyme systems. The IC50 for benzydamine against thromboxane synthetase was 100 mug/ml and 250 mug/ml against RSV. Indomethacin showed a greater degree of selectivity with an IC50 of 5 mug/ml for the ram seminal vesicle cyclo-oxygenase compared to 100 mug/ml for thromboxane synthetase.", "contents": "Prostaglandin endoperoxide and thromboxane generating systems and their selective inhibition. Two enzyme systems and their selective inhibition are described. Microsomes from ram seminal vesicles (RSV) incubated with arachidonic acid at 22 degrees C generated a rabbit aorta contracting substance which, after rapid ether extraction, had characteristics similar to purified standard endoperoxides. Incubation of either purified endoperoxide or the product from RSV and arachidonic acid with horse platelet microsomes (HPM) yielded a more potent rabbit aorta contracting substance characterized as thromboxane A2, with a half life of 35.9 +/- 2.2 s at 37 degrees C after ether extraction. Two inhibitors, indomethacin and benzydamine exhibited selectivity for the two enzyme systems. The IC50 for benzydamine against thromboxane synthetase was 100 mug/ml and 250 mug/ml against RSV. Indomethacin showed a greater degree of selectivity with an IC50 of 5 mug/ml for the ram seminal vesicle cyclo-oxygenase compared to 100 mug/ml for thromboxane synthetase.", "PMID": 968048}
{"id": "pubmed23n0032_7207", "title": "Paradoxical endogenous synthesis of a coronary dilating substance from arachidonate.", "content": "Isolated bovine, canine, and human coronary arteries exhibited dose dependent contractions to prostaglandin (PG) E2 and F2alpha (50 ng/ml to 10mug/ml). The ED50 value for both PGE2 and PGF2alpha was 500 ng/ml in the bovine and human coronary arteries. Paradoxically, although PGE2 and gf2alpha are vasoconstrictors, administration of their precursor, arachidonate (100 ng/ml to 10 mug/ml) caused relaxation of the bovine, canine and human coronary arteries. This observation suggests that arachidonate is not being converted by the coronary PG synthetase to PGE2 or PGF2alpha. However, the arachidonate induced coronary relaxation was inhibited by pretreatment with PG synthetase inhibitors, indomethacin, meclofenemate and aspirin. Indomethacin addition to the strips previously relaxed by arachidonate caused contraction. In contrast to other PGs (E2 and F2alpha), PGE1 (10 ng/ml to 10 mug/ml) caused dose dependent relaxation of the bovine coronary arteries (ED50 = 100 ng/ml). Indomethacin induced further relaxation of the blood vessels previously relaxed by PGE1. Since PGE1 cannot arise from arachidonate, the arachidonate coronary dilation and reversal by indomethacin must be independent of PGE1 formation. Linolenate (100 ng/ml to 10 mug/ml) and oleate (100 ng/ml to 10 mug/ml) also caused relaxation of the bovine coronary blood vessels both before and after indomethacin, thereby eliminating a direct non-specific fatty acid effect as the cause of the arachidonate relaxation. These results suggest that in isolated coronaries, arachidonate undergoes a novel conversion, possibly by PG synthetase, to a dilating substance which exerts different contractile effects than exogenously administered PGE2, PGF2alpha and PGE1.", "contents": "Paradoxical endogenous synthesis of a coronary dilating substance from arachidonate. Isolated bovine, canine, and human coronary arteries exhibited dose dependent contractions to prostaglandin (PG) E2 and F2alpha (50 ng/ml to 10mug/ml). The ED50 value for both PGE2 and PGF2alpha was 500 ng/ml in the bovine and human coronary arteries. Paradoxically, although PGE2 and gf2alpha are vasoconstrictors, administration of their precursor, arachidonate (100 ng/ml to 10 mug/ml) caused relaxation of the bovine, canine and human coronary arteries. This observation suggests that arachidonate is not being converted by the coronary PG synthetase to PGE2 or PGF2alpha. However, the arachidonate induced coronary relaxation was inhibited by pretreatment with PG synthetase inhibitors, indomethacin, meclofenemate and aspirin. Indomethacin addition to the strips previously relaxed by arachidonate caused contraction. In contrast to other PGs (E2 and F2alpha), PGE1 (10 ng/ml to 10 mug/ml) caused dose dependent relaxation of the bovine coronary arteries (ED50 = 100 ng/ml). Indomethacin induced further relaxation of the blood vessels previously relaxed by PGE1. Since PGE1 cannot arise from arachidonate, the arachidonate coronary dilation and reversal by indomethacin must be independent of PGE1 formation. Linolenate (100 ng/ml to 10 mug/ml) and oleate (100 ng/ml to 10 mug/ml) also caused relaxation of the bovine coronary blood vessels both before and after indomethacin, thereby eliminating a direct non-specific fatty acid effect as the cause of the arachidonate relaxation. These results suggest that in isolated coronaries, arachidonate undergoes a novel conversion, possibly by PG synthetase, to a dilating substance which exerts different contractile effects than exogenously administered PGE2, PGF2alpha and PGE1.", "PMID": 968049}
{"id": "pubmed23n0032_7208", "title": "Studies on the effects of prostaglandins E1, E2, A1, and A2 on the dustus arteriosus of swine in vivo using cineangiography.", "content": "Prostaglandins E1, E2, A1, and A2 have been shown by cineaortography to open and dilate the ductus arteriosus in anesthetised piglets 3 to 6 hours of age. The dosage of PGEs required was 1 to 4 mug/kg/min. and of PGAs 20 to 40 mug/kg/min. The effect of PGEs faded within 20 minutes of stopping infusion but the effect of PGAs was still evident up to 45 minutes after stopping the infusion. Little effect was noted from hypoxia or from the addition of indomethacin to prostaglandin infusion. Side effects were not troublesome with the dosage employed but hypotension and apnea sometimes occurred at the onset of PGE infusions.", "contents": "Studies on the effects of prostaglandins E1, E2, A1, and A2 on the dustus arteriosus of swine in vivo using cineangiography. Prostaglandins E1, E2, A1, and A2 have been shown by cineaortography to open and dilate the ductus arteriosus in anesthetised piglets 3 to 6 hours of age. The dosage of PGEs required was 1 to 4 mug/kg/min. and of PGAs 20 to 40 mug/kg/min. The effect of PGEs faded within 20 minutes of stopping infusion but the effect of PGAs was still evident up to 45 minutes after stopping the infusion. Little effect was noted from hypoxia or from the addition of indomethacin to prostaglandin infusion. Side effects were not troublesome with the dosage employed but hypotension and apnea sometimes occurred at the onset of PGE infusions.", "PMID": 968050}
{"id": "pubmed23n0032_7209", "title": "Indomethacin inhibits responses to all vasoconstrictors in the rat mesenteric vascular bed: restoration of responses by prostaglandin E2.", "content": "Indomethacin added to the perfusing buffer inhibited pressor responses to noradrenaline, angiotensin II, arginine vasopressin, histamine, serotonin, calcium ions and potassium ions in the male rat mesenteric vascular bed. For every pressor agent the indomethacin concentration which inhibited response amplitude by 50% was about 7 microg/ml (2.1 X 10(-5) M). With every pressor agent, prostaglandin (PG) E2 could restore normal responsiveness in indomethacin-blocked preparations even while the indomethacin was still present in the buffer. The concentration of PGE2 required was proportional to the concentration of indomethacin. Preparations completely inhibited by indomethacin needed about 5ng/ml PGE2 for complete restoration of normal responses. Aspirin and mefenamic acid could also inhibit responses to all pressor agents tested but with these drugs only a partial restoration could be achieved by PGE2.", "contents": "Indomethacin inhibits responses to all vasoconstrictors in the rat mesenteric vascular bed: restoration of responses by prostaglandin E2. Indomethacin added to the perfusing buffer inhibited pressor responses to noradrenaline, angiotensin II, arginine vasopressin, histamine, serotonin, calcium ions and potassium ions in the male rat mesenteric vascular bed. For every pressor agent the indomethacin concentration which inhibited response amplitude by 50% was about 7 microg/ml (2.1 X 10(-5) M). With every pressor agent, prostaglandin (PG) E2 could restore normal responsiveness in indomethacin-blocked preparations even while the indomethacin was still present in the buffer. The concentration of PGE2 required was proportional to the concentration of indomethacin. Preparations completely inhibited by indomethacin needed about 5ng/ml PGE2 for complete restoration of normal responses. Aspirin and mefenamic acid could also inhibit responses to all pressor agents tested but with these drugs only a partial restoration could be achieved by PGE2.", "PMID": 968051}
{"id": "pubmed23n0032_7210", "title": "Radioimmunoassay of prostaglandins A and B in human blood.", "content": "A highly sensitive radioimmunoassay for the measurement of plasma prostaglandins A and B, expressed in equivalents of PGA1, is described. This method was used for the measurement of prostaglandins A and B (PGA/B) in 23 healthy volunteers and 25 hypertensive patients. The PGA/B concentration in peripheral venous plasma of 23 healthy normotensive subjects is 115 +/- 15 pg/ml. The repeated measurement of the same plasma samples kept frozen for 60 days at -20 degrees C shows mean 194% increase of PGA/B concentration. The major site of synthesis of PGA/B seems to be the kidney. However in two patients PGA/B concentration in arterial blood was greater than in venous blood suggesting the possibility of cardio-pulmonary synthesis. The major site of inactivation is the hepatic circulation, as PGA/B concentration in hepatal venous blood is by 30% lower than in vena caval blood. The arterial concentration is 3% lower than venous PGA/B demonstrating very low pulmonary inactivation. Therefore the prostaglandins of the A and B series may represent a \"circulating hormone\". The plasmatic PGA/B is significantly increased in reno-vascular and essential hypertension.", "contents": "Radioimmunoassay of prostaglandins A and B in human blood. A highly sensitive radioimmunoassay for the measurement of plasma prostaglandins A and B, expressed in equivalents of PGA1, is described. This method was used for the measurement of prostaglandins A and B (PGA/B) in 23 healthy volunteers and 25 hypertensive patients. The PGA/B concentration in peripheral venous plasma of 23 healthy normotensive subjects is 115 +/- 15 pg/ml. The repeated measurement of the same plasma samples kept frozen for 60 days at -20 degrees C shows mean 194% increase of PGA/B concentration. The major site of synthesis of PGA/B seems to be the kidney. However in two patients PGA/B concentration in arterial blood was greater than in venous blood suggesting the possibility of cardio-pulmonary synthesis. The major site of inactivation is the hepatic circulation, as PGA/B concentration in hepatal venous blood is by 30% lower than in vena caval blood. The arterial concentration is 3% lower than venous PGA/B demonstrating very low pulmonary inactivation. Therefore the prostaglandins of the A and B series may represent a \"circulating hormone\". The plasmatic PGA/B is significantly increased in reno-vascular and essential hypertension.", "PMID": 968052}
{"id": "pubmed23n0032_7211", "title": "Mechanism of steroid action in inflammation: inhibition of prostaglandin synthesis and release.", "content": "Acute arthritis was induced by injection of cell-free extract of group A Streptococci into the knee joints of mature male rats. Slices of control and inflamed synovia were incubated for 30 to 240 minutes and the rate of prostaglandin E (PGE) released into the medium was measured by radioimmunoassay. PGE release from inflamed synovia was 5-8 fold higher than that in normal tissue. Incubation of inflamed synovia with corticosterone acetate, dexamethasone or prednisone (100 mug/ml) for one or four hours reduced PGE release by 33% and 55% respectively. Lower concentrations of corticosterone (10 - 30 mug/ml) were ineffective. Aldosterone and progesterone (100 mug/ml) had no effect on PGE release throughout the incubation period. Chloroquine (10 mug/ml) inhibited PGE release from inflamed synovia by 50%. Indomethacin (1 mug/ml) abolished PGE release by 90%. Corticosterone, dexamethasone and prednisone reduced PGE content of inflamed synovia by approximately 45% during a 4-h incubation period. Aldosterone and progesterone were ineffective, while indomethacin reduced PGE content by 70%. The suppressive action of corticosterone on PGE release was prevented by addition to the medium of arachidonic acid (2 mug/ml). By contrast, the inhibitory action of indomethacin was not affected by provision of exogenous substrate. We suggest that glucocorticosteroids reduce PGE release by limiting the availability of the substrate for prostaglandin biosynthesis, and this may well explain some of their anti-inflammatory properties.", "contents": "Mechanism of steroid action in inflammation: inhibition of prostaglandin synthesis and release. Acute arthritis was induced by injection of cell-free extract of group A Streptococci into the knee joints of mature male rats. Slices of control and inflamed synovia were incubated for 30 to 240 minutes and the rate of prostaglandin E (PGE) released into the medium was measured by radioimmunoassay. PGE release from inflamed synovia was 5-8 fold higher than that in normal tissue. Incubation of inflamed synovia with corticosterone acetate, dexamethasone or prednisone (100 mug/ml) for one or four hours reduced PGE release by 33% and 55% respectively. Lower concentrations of corticosterone (10 - 30 mug/ml) were ineffective. Aldosterone and progesterone (100 mug/ml) had no effect on PGE release throughout the incubation period. Chloroquine (10 mug/ml) inhibited PGE release from inflamed synovia by 50%. Indomethacin (1 mug/ml) abolished PGE release by 90%. Corticosterone, dexamethasone and prednisone reduced PGE content of inflamed synovia by approximately 45% during a 4-h incubation period. Aldosterone and progesterone were ineffective, while indomethacin reduced PGE content by 70%. The suppressive action of corticosterone on PGE release was prevented by addition to the medium of arachidonic acid (2 mug/ml). By contrast, the inhibitory action of indomethacin was not affected by provision of exogenous substrate. We suggest that glucocorticosteroids reduce PGE release by limiting the availability of the substrate for prostaglandin biosynthesis, and this may well explain some of their anti-inflammatory properties.", "PMID": 968054}
{"id": "pubmed23n0032_7212", "title": "Changes in pituitary hormone secretions and corpora lutea following PGF2alpha administration to PMS/hCG-stimulated rats.", "content": "Pseudopregnancy was induced in 26 day old female rats by giving 30 IU of PMS followed 56 hours later with 5 IU of hCG. Day 1 of pseudopregnancy was considered established 72 hours after PMS administration. Pseudopregnancy lasted 14 to 15 days. Ovarian weights increased for 4 to 8 times due to treatment. Histological examiniation of the corpora lutea (CL) of pseudopregnancy suggested luteolysis began on day 6 and extended to day 8. A \"new\" crop of CL appeared on day 9 suggesting the duration of pseudopregnancy was supported by more than one generation of CL or by CL maturing at different rates. Twice daily administration of 1 mg PGF2alpha on days 5 to 8 prevented the appearance of the \"new\" CL on day 9, and increased signs of luteolysis in the initially formed CL. Lower doses (0.01 and 0.1 mg, b.i.d., X 4 days) delayed the appearance of \"new\" CL until day 10. Blood samples withdrawn between 0930 and 1100 hours were analysed for FSH, LH and prolactin. Animals treated with 0.01 and 0.1 mg of PGF2alpha, b.i.d., X 4 days had increased LH values on day 8, thus the PG appeared luteotrophic. Rats treated with 1 mg of PGF2alpha, b.i.d., X 4 days had decreased LH values on day 7 and the CL showed subsequent luteolysis. FSH levels were relatively constant during pseudopregnancy. However, all doses of PGF2alpha reduced FSH levels on day 7. An associated decrease in uterine weight occurred, possibly due to reduced follicular development. Prolactin levels fell in response to PGF2alpha treatment which undoubtedly contributed to the observed luteolysis. The signs of early cessation of pseudopregnancy were: increased serum FSH on days 9 and 10; increasing uterine weight; and the reappearance of follicular growth. These data suggest that PGF2alpha reduced the duration of pseudopregnancy primarily by inhibiting the secretion of FSH, LH and prolactin.", "contents": "Changes in pituitary hormone secretions and corpora lutea following PGF2alpha administration to PMS/hCG-stimulated rats. Pseudopregnancy was induced in 26 day old female rats by giving 30 IU of PMS followed 56 hours later with 5 IU of hCG. Day 1 of pseudopregnancy was considered established 72 hours after PMS administration. Pseudopregnancy lasted 14 to 15 days. Ovarian weights increased for 4 to 8 times due to treatment. Histological examiniation of the corpora lutea (CL) of pseudopregnancy suggested luteolysis began on day 6 and extended to day 8. A \"new\" crop of CL appeared on day 9 suggesting the duration of pseudopregnancy was supported by more than one generation of CL or by CL maturing at different rates. Twice daily administration of 1 mg PGF2alpha on days 5 to 8 prevented the appearance of the \"new\" CL on day 9, and increased signs of luteolysis in the initially formed CL. Lower doses (0.01 and 0.1 mg, b.i.d., X 4 days) delayed the appearance of \"new\" CL until day 10. Blood samples withdrawn between 0930 and 1100 hours were analysed for FSH, LH and prolactin. Animals treated with 0.01 and 0.1 mg of PGF2alpha, b.i.d., X 4 days had increased LH values on day 8, thus the PG appeared luteotrophic. Rats treated with 1 mg of PGF2alpha, b.i.d., X 4 days had decreased LH values on day 7 and the CL showed subsequent luteolysis. FSH levels were relatively constant during pseudopregnancy. However, all doses of PGF2alpha reduced FSH levels on day 7. An associated decrease in uterine weight occurred, possibly due to reduced follicular development. Prolactin levels fell in response to PGF2alpha treatment which undoubtedly contributed to the observed luteolysis. The signs of early cessation of pseudopregnancy were: increased serum FSH on days 9 and 10; increasing uterine weight; and the reappearance of follicular growth. These data suggest that PGF2alpha reduced the duration of pseudopregnancy primarily by inhibiting the secretion of FSH, LH and prolactin.", "PMID": 968055}
{"id": "pubmed23n0032_7213", "title": "Extra-amniotic 15 (S)-15 methyl PGF2alpha to induce abortion: a study of three administration schedules.", "content": "Abortion was induced in 60 patients between 8 and 18 weeks gestation using 15(S)-15 methyl PGF2alpha in one of three extra-amniotic administration schedules: 1.0 mg in viscous medium (Tylose), 1 mg in viscous medium (Hyskon) or 0.5 mg in non-viscous medium repeated at 12 hours. Eighty per cent of patients aborted within 24 hours in each group. The overall mean induction-abortion interval (+/- S.E.) was 17.6 +/- 2.0: there was no significant difference between the three groups. Twenty patients treated with 1.0 mg in viscous medium had the catheter removed immediately following the prostaglandin injection and the success rate was not significantly altered. Gastro-intestinal side effects (vomiting in 50%, diarrhoea in 32.5%) were more frequent in the patients treated with the larger dose though the difference was not statistically significant. No significant haematological or biochemical changes were detected during the 24 hours following the start of treatment in 24 patients investigated. Thirty seven of the 60 patients (61.5%) aborted completely and did not require surgical evacuation, and none lost more than 500 ml of blood, nor required transfusion. It is concluded that abortion can be induced with a single extra-amniotic injection of 1 mg of 15(S)-15 methyl PGF2alpha in viscous medium in a large percentage of patients but that the incidence of side effects is high.", "contents": "Extra-amniotic 15 (S)-15 methyl PGF2alpha to induce abortion: a study of three administration schedules. Abortion was induced in 60 patients between 8 and 18 weeks gestation using 15(S)-15 methyl PGF2alpha in one of three extra-amniotic administration schedules: 1.0 mg in viscous medium (Tylose), 1 mg in viscous medium (Hyskon) or 0.5 mg in non-viscous medium repeated at 12 hours. Eighty per cent of patients aborted within 24 hours in each group. The overall mean induction-abortion interval (+/- S.E.) was 17.6 +/- 2.0: there was no significant difference between the three groups. Twenty patients treated with 1.0 mg in viscous medium had the catheter removed immediately following the prostaglandin injection and the success rate was not significantly altered. Gastro-intestinal side effects (vomiting in 50%, diarrhoea in 32.5%) were more frequent in the patients treated with the larger dose though the difference was not statistically significant. No significant haematological or biochemical changes were detected during the 24 hours following the start of treatment in 24 patients investigated. Thirty seven of the 60 patients (61.5%) aborted completely and did not require surgical evacuation, and none lost more than 500 ml of blood, nor required transfusion. It is concluded that abortion can be induced with a single extra-amniotic injection of 1 mg of 15(S)-15 methyl PGF2alpha in viscous medium in a large percentage of patients but that the incidence of side effects is high.", "PMID": 968056}
{"id": "pubmed23n0032_7214", "title": "Menstrual induction by the vaginal application of ICI 81008 gel.", "content": "After approximately 2 weeks menstrual delay (positive Pregnosticon Tests) \"menstrual induction\" was attempted in 75 gravidas by repeated vaginal application of a gel, containing 200 or 400 mug/ml ICI 81008. After approximately 10 minutes, following the 1st vaginal delivery of 400 mug ICI 81008, the uterus responded to this PGF2alpha analogue with sustained contracture. The highest success rate in induced bleeding (93%) and pregnancy termination (79%), without supportive therapy, was achieved when 400 mug ICI 81008 was administered 2 to 5 times at 4 hour intervals. Those gravidas (21%), who failed in induced menstruation, or stopped bleeding within 24 hour- after treatment, had positive Pregnosticon Tests on day 14 and were curetted. The side effects, mostly vomiting and increased blood pressure, were transient and subjectively and medically acceptable. While the vaginal application of the drug is apparently less effective than the intrauterine (1), it has the advantage of simple delivery and the potential of self-administration.", "contents": "Menstrual induction by the vaginal application of ICI 81008 gel. After approximately 2 weeks menstrual delay (positive Pregnosticon Tests) \"menstrual induction\" was attempted in 75 gravidas by repeated vaginal application of a gel, containing 200 or 400 mug/ml ICI 81008. After approximately 10 minutes, following the 1st vaginal delivery of 400 mug ICI 81008, the uterus responded to this PGF2alpha analogue with sustained contracture. The highest success rate in induced bleeding (93%) and pregnancy termination (79%), without supportive therapy, was achieved when 400 mug ICI 81008 was administered 2 to 5 times at 4 hour intervals. Those gravidas (21%), who failed in induced menstruation, or stopped bleeding within 24 hour- after treatment, had positive Pregnosticon Tests on day 14 and were curetted. The side effects, mostly vomiting and increased blood pressure, were transient and subjectively and medically acceptable. While the vaginal application of the drug is apparently less effective than the intrauterine (1), it has the advantage of simple delivery and the potential of self-administration.", "PMID": 968057}
{"id": "pubmed23n0032_7215", "title": "Evaluation of vaginal delivery systems containing 15[s]15-methyl PGF2alpha methyl ester.", "content": "Silicone vaginal delivery systems containing 15[S]15-methyl-PGF2alpha methyl ester have been evaluated in vitro, and in vivo in the rhesus monkey. Three types of vaginal devices have been formulated to contain different concentrations of drug. The cumulative amount of 15[s]15-methyl-PGF2alpha methyl ester released in vitro from a planar silicone rubber matrix was dependent upon the initial loading dose of the prostaglandin. Drug-containing vaginal rings were evaluated in early pregnant and mid-trimester pregnant monkeys. Within 10 min after ring administration there was an increase in the amplitude and frequency of contractions. In mid-trimester pregnant animals there was an initial increase in plasma progesterone and then a decrease following treatment, while there was a progressive decrease in plasma progesterone in early pregnant animals. All 6 mid-trimester pregnant monkeys treated with vaginal rings aborted. Pregnancy was terminated in 4 of 5 early pregnant monkeys treated with vaginal rings. Blood levels of 15-methyl-PGF2alpha rose rapidly to attain a peak 1 hr after treatment with vaginal rings. After the peak, plasma levels of 15-methul-PGF2alpha declined to a plateau which was fairly constant between 2 and 8 hr. Vaginal silicone-gelatin laminates containing 15[s]15-methyl-PGF2alpha methyl ester were also effective in causing an increase in uterine muscle activity and terminating pregnancy in mid-trimester in pregnant monkeys. Plasma progesterone also increased shortly after treatment in these animals, and then declined after the peak at 8 hr. The plasma profile of 15-methyl-PGF2alpha when animals were treated with these vaginal laminates was different from that observed following ring treatment. In pregnant animals the concentration of 15-methyl-PGF2alpha rose more slowly following laminate insertion, and the peak values were considerably less than those following ring treatment. Vaginal devices used in monkeys and humans caused plasma concentrations of 15-methyl-PGF2alpha that were generally lower than those observed in monkeys treated with either vaginal rings or laminates. These studies have demonstrated that silicone vaginal delivery systems containing 15[S]15-methyl-PGF2alpha methyl ester result in sustained and fairly constant plasma levels of 15-methyl-PGF2alpha, and terminate both 1st- and 2nd-trimester pregnancies in the monkey. The vaginal device offers the possibility for self-administration of PGs for early pregnancy termination and menstrual cycle regulation.", "contents": "Evaluation of vaginal delivery systems containing 15[s]15-methyl PGF2alpha methyl ester. Silicone vaginal delivery systems containing 15[S]15-methyl-PGF2alpha methyl ester have been evaluated in vitro, and in vivo in the rhesus monkey. Three types of vaginal devices have been formulated to contain different concentrations of drug. The cumulative amount of 15[s]15-methyl-PGF2alpha methyl ester released in vitro from a planar silicone rubber matrix was dependent upon the initial loading dose of the prostaglandin. Drug-containing vaginal rings were evaluated in early pregnant and mid-trimester pregnant monkeys. Within 10 min after ring administration there was an increase in the amplitude and frequency of contractions. In mid-trimester pregnant animals there was an initial increase in plasma progesterone and then a decrease following treatment, while there was a progressive decrease in plasma progesterone in early pregnant animals. All 6 mid-trimester pregnant monkeys treated with vaginal rings aborted. Pregnancy was terminated in 4 of 5 early pregnant monkeys treated with vaginal rings. Blood levels of 15-methyl-PGF2alpha rose rapidly to attain a peak 1 hr after treatment with vaginal rings. After the peak, plasma levels of 15-methul-PGF2alpha declined to a plateau which was fairly constant between 2 and 8 hr. Vaginal silicone-gelatin laminates containing 15[s]15-methyl-PGF2alpha methyl ester were also effective in causing an increase in uterine muscle activity and terminating pregnancy in mid-trimester in pregnant monkeys. Plasma progesterone also increased shortly after treatment in these animals, and then declined after the peak at 8 hr. The plasma profile of 15-methyl-PGF2alpha when animals were treated with these vaginal laminates was different from that observed following ring treatment. In pregnant animals the concentration of 15-methyl-PGF2alpha rose more slowly following laminate insertion, and the peak values were considerably less than those following ring treatment. Vaginal devices used in monkeys and humans caused plasma concentrations of 15-methyl-PGF2alpha that were generally lower than those observed in monkeys treated with either vaginal rings or laminates. These studies have demonstrated that silicone vaginal delivery systems containing 15[S]15-methyl-PGF2alpha methyl ester result in sustained and fairly constant plasma levels of 15-methyl-PGF2alpha, and terminate both 1st- and 2nd-trimester pregnancies in the monkey. The vaginal device offers the possibility for self-administration of PGs for early pregnancy termination and menstrual cycle regulation.", "PMID": 968060}
{"id": "pubmed23n0032_7216", "title": "The use of silastic vaginal device containing (15S)-15 methyl prostaglandin F2 alpha methyl ester for early first trimester pregnancy termination.", "content": "(15 S) - 15 methyl prostaglandin F2 alpha methyl ester in a silastic vaginal device was administered to 9 gravid women in the first 7 weeks of gestation for elective termination of pregnancy. Continuous intrauterine pressure recording was carried out in one case. Prompt initiation of uterine activity and vaginal bleeding was seen and expulsion of products of conception occurred in under 24 hours in all cases. There were no serious complications and side effects were considered acceptable. Two patients have required subsequent curettage for removal of retained placental tissue. This incidence of failure to complete expulsion, if confirmed in a larger series, is considered to render this technique without modification unacceptable as a routine method for early pregnancy termination.", "contents": "The use of silastic vaginal device containing (15S)-15 methyl prostaglandin F2 alpha methyl ester for early first trimester pregnancy termination. (15 S) - 15 methyl prostaglandin F2 alpha methyl ester in a silastic vaginal device was administered to 9 gravid women in the first 7 weeks of gestation for elective termination of pregnancy. Continuous intrauterine pressure recording was carried out in one case. Prompt initiation of uterine activity and vaginal bleeding was seen and expulsion of products of conception occurred in under 24 hours in all cases. There were no serious complications and side effects were considered acceptable. Two patients have required subsequent curettage for removal of retained placental tissue. This incidence of failure to complete expulsion, if confirmed in a larger series, is considered to render this technique without modification unacceptable as a routine method for early pregnancy termination.", "PMID": 968061}
{"id": "pubmed23n0032_7217", "title": "First experiences with a (15S)-15-methyl prostaglandin F2alpha vaginal device for termination of early first trimester pregnancy with serial sonographic observations.", "content": "A silastic device containing 10 mg (15S)-15-Methyl Prostaglandin F2alpha was administered vaginally in three parous patients under 50 days gestation who requested therapeutic abortion. Painful contractions appeared within one hour and vaginal bleeding within three hours of application. All patients aborted, although in one tissue had to be removed from the uterus. Plasma progesterone concentrations were measured in two patients: they decreased within four hours, then increased slightly and finally decreased 24 hours after administration of the device. Nausea, vomiting, and diarrhea were observed in all three patients. The estimated blood loss was less than 100 ml and no significant hematocrit changes were observed in all patients. In serial sonographic examinations of one patient, the fragmentation and dislodgment of the gestational sac could be observed within six hours after administration of the device. After these albeit limited experiences, it is concluded that the (15S)-15-Methyl PGF2alpha vaginal device is of great potential value for induction of early first trimester abortion.", "contents": "First experiences with a (15S)-15-methyl prostaglandin F2alpha vaginal device for termination of early first trimester pregnancy with serial sonographic observations. A silastic device containing 10 mg (15S)-15-Methyl Prostaglandin F2alpha was administered vaginally in three parous patients under 50 days gestation who requested therapeutic abortion. Painful contractions appeared within one hour and vaginal bleeding within three hours of application. All patients aborted, although in one tissue had to be removed from the uterus. Plasma progesterone concentrations were measured in two patients: they decreased within four hours, then increased slightly and finally decreased 24 hours after administration of the device. Nausea, vomiting, and diarrhea were observed in all three patients. The estimated blood loss was less than 100 ml and no significant hematocrit changes were observed in all patients. In serial sonographic examinations of one patient, the fragmentation and dislodgment of the gestational sac could be observed within six hours after administration of the device. After these albeit limited experiences, it is concluded that the (15S)-15-Methyl PGF2alpha vaginal device is of great potential value for induction of early first trimester abortion.", "PMID": 968062}
{"id": "pubmed23n0032_7218", "title": "Induction of abortion by vaginal administration of 15(s)15-methyl prostaglandin F2alpha methyl ester. A comparison of two delivery systems.", "content": "15-methyl PGF2alpha methyl ester in a slow releasing vaginal device was administered to 30 women in the first and second trimester of gestation. Ten women were early pregnant (31-49 days following the last menstrual period) and the remaining 20 patients in the 10th to 20th week of gestation. The first group received either a device containing 10 mg 0.5% 15-methyl PGF2alpha methyl ester or a half of that device and the second group either a 0.5% or 1% device. The results were compared with those found following repeated vaginal administration of triglycerides suppositories, containing 15-methyl PGF2alpha methyl ester, to 50 early pregnant patients and 30 patients in the second trimester of gestation. The mean total dose of the compound given to these two groups was 3.9 and 7.8 mg respectively. All the 60 early pregnant patients aborted following treatment judged from clinical course and decreasing HCG values. Bleeding started as a rule three to six hours following the start of treatment and continued for 10 to 14 days. In two patients the abortion was incomplete. The side effects in the patients who received half the 0.5% device was comparable to those following the repeated vaginal suppositories while in the patients who obtained the whole 0.5% device, the frequency of side effects was increased indicating that an unnecessary high dose was given. In all the three groups of patients in the late first and second trimester of pregnancy, treatment resulted in abortion within 24 hours in 90% of the patients. In the 1% device an accumulation of side effects was found during the first hours following the start of treatment probably due to an initial, more rapid absorption of 15-methyl PGF2alpha methyl ester. With the repeated administration and with the 0.5% device, the side effects occurred mainly in the hours prior to abortion. However, in all three groups of patients the side effects were within acceptable levels. These preliminary results indicate that further development on the device may result in an effective one vaginal administration treatment for termination of both first and second trimester pregnancies.", "contents": "Induction of abortion by vaginal administration of 15(s)15-methyl prostaglandin F2alpha methyl ester. A comparison of two delivery systems. 15-methyl PGF2alpha methyl ester in a slow releasing vaginal device was administered to 30 women in the first and second trimester of gestation. Ten women were early pregnant (31-49 days following the last menstrual period) and the remaining 20 patients in the 10th to 20th week of gestation. The first group received either a device containing 10 mg 0.5% 15-methyl PGF2alpha methyl ester or a half of that device and the second group either a 0.5% or 1% device. The results were compared with those found following repeated vaginal administration of triglycerides suppositories, containing 15-methyl PGF2alpha methyl ester, to 50 early pregnant patients and 30 patients in the second trimester of gestation. The mean total dose of the compound given to these two groups was 3.9 and 7.8 mg respectively. All the 60 early pregnant patients aborted following treatment judged from clinical course and decreasing HCG values. Bleeding started as a rule three to six hours following the start of treatment and continued for 10 to 14 days. In two patients the abortion was incomplete. The side effects in the patients who received half the 0.5% device was comparable to those following the repeated vaginal suppositories while in the patients who obtained the whole 0.5% device, the frequency of side effects was increased indicating that an unnecessary high dose was given. In all the three groups of patients in the late first and second trimester of pregnancy, treatment resulted in abortion within 24 hours in 90% of the patients. In the 1% device an accumulation of side effects was found during the first hours following the start of treatment probably due to an initial, more rapid absorption of 15-methyl PGF2alpha methyl ester. With the repeated administration and with the 0.5% device, the side effects occurred mainly in the hours prior to abortion. However, in all three groups of patients the side effects were within acceptable levels. These preliminary results indicate that further development on the device may result in an effective one vaginal administration treatment for termination of both first and second trimester pregnancies.", "PMID": 968064}
{"id": "pubmed23n0032_7219", "title": "Measurement of (15S)-15-methyl prostaglandin F2alpha by radioimmunoassay.", "content": "Antibodies against (15s)-15-methyl prostaglandin F2alpha (15-MF) were produced in rabbits immunized with a 15-MF bovine serum albumin conjugate. Tritium labeled 15-MF was prepared from (15S)-15-methyl prostaglandin E2 by reduction with tritiated sodium borohydride. Antiserum specificity and label specific activity (12 Ci/mM) were sfficient to enable development of a sensitive, accurate and relatively specific radioimmunoassay for this prostaglandin analog. The assay can detect as little as five picograms of 15-MF in 100 mul of unextracted blood plasma. Endogenous concentrations of PGF2alpha should not cause a significant blank using current assay conditions, as cross reactivity with this molecule was less than one percent. There is, however, significant affinity (45%) for the C-1 methyl ester of 15-MF. This makes the assay also applicable for measuring this molecule, although no distinction can be made between it and the free acid when neat plasma is assayed. Plasma samples from human and monkey studies were assayed for 15-MF before and after recipients were treated with pharmacologically active doses of this compound. Blank values (0 Hour) of less than 0.1 nanograms per ml were found for all pre-treatment samples while post-treatment samples contained detectable levels. Results of the human study was compared and found to be in excellent agreement with values that were obtained using a gas-liquid chromatographic - mass spectrographic assay.", "contents": "Measurement of (15S)-15-methyl prostaglandin F2alpha by radioimmunoassay. Antibodies against (15s)-15-methyl prostaglandin F2alpha (15-MF) were produced in rabbits immunized with a 15-MF bovine serum albumin conjugate. Tritium labeled 15-MF was prepared from (15S)-15-methyl prostaglandin E2 by reduction with tritiated sodium borohydride. Antiserum specificity and label specific activity (12 Ci/mM) were sfficient to enable development of a sensitive, accurate and relatively specific radioimmunoassay for this prostaglandin analog. The assay can detect as little as five picograms of 15-MF in 100 mul of unextracted blood plasma. Endogenous concentrations of PGF2alpha should not cause a significant blank using current assay conditions, as cross reactivity with this molecule was less than one percent. There is, however, significant affinity (45%) for the C-1 methyl ester of 15-MF. This makes the assay also applicable for measuring this molecule, although no distinction can be made between it and the free acid when neat plasma is assayed. Plasma samples from human and monkey studies were assayed for 15-MF before and after recipients were treated with pharmacologically active doses of this compound. Blank values (0 Hour) of less than 0.1 nanograms per ml were found for all pre-treatment samples while post-treatment samples contained detectable levels. Results of the human study was compared and found to be in excellent agreement with values that were obtained using a gas-liquid chromatographic - mass spectrographic assay.", "PMID": 968065}
{"id": "pubmed23n0032_7220", "title": "Hormone release and abortifacient effectiveness of a newly developed silastic device containing 15-ME-PGF2alpha methyl ester in concentrations of 0.5% and 1.0%.", "content": "Abortion was successfully induced by intravaginal administration of a newly developed silastic device impregnated with 15(S)-15-methyl-prostaglandin F2alpha methyl ester in a concentration of 0.5% in 26 of 35 women in the 12th to the 24th weeks of gestation and by prostaglandin with concomitant oxytocin infusion in an additional 5 patients. A second comparable group of 35 women, with gestational ages from 9 to 23 weeks, received a silastic device with a concentration of 1.0% of the prostaglandin. Twenty-six women in the second group aborted with prostaglandin alone and an additional 6 patients aborted with combined prostaglandin-oxytocin therapy. Seven patients of the 70 included in this study failed to abort with intravaginal administration of 15-ME-PGF2alpha methyl ester even with concomitant oxytocin therapy; this group included 4 patients receiving the 0.5% device and 3 patients with the 1.0% device. The mean abortion time for the 31 successful abortions with 0.5% device was 15.15 hours and multiparous patients aborted in a mean time of 9.69 hours, significantly faster than the nulliparous patients, mean time 18.49 hours. The mean abortion time for the 32 successful abortions with the 1.0% device was 15.61 hours and there was no significant difference in the mean abortion times between nulliparous and multiparous patients in this group. No significant difference could be demonstrated between the mean abortion times of patients receiving an 0.5% concentrations of prostaglandin and patients receiving a 1.0% concentration of the drug. In both groups more than 40% of the patients had aborted within 12 hours of the insertion of the prostaglandin device and more than 80% had aborted within 24 hours. The intravaginal device appeared to be effective in inducing abortion throughout the gestational ages tested in this series. Peripheral plasma levels of 15-ME-PGF2alpha were analyzed in 15 patients with the 0.5% device and 8 patients with the 1.0% device. In patients with the 0.5% device the mean plasma concentration of prostaglandin peaked 2 hours after insertion, while in patients with the 1.0% device the peak was achieved within 15 minutes of insertion followed by a rapid decline in plasma prostaglandin levels. Diarrhea was the most frequent side effect related to the intravaginal administration of the prostaglandin, and in this series the severity of this gastro-intestinal disturbance appeared to be significantly higher in patients receiving the 1.0% device.", "contents": "Hormone release and abortifacient effectiveness of a newly developed silastic device containing 15-ME-PGF2alpha methyl ester in concentrations of 0.5% and 1.0%. Abortion was successfully induced by intravaginal administration of a newly developed silastic device impregnated with 15(S)-15-methyl-prostaglandin F2alpha methyl ester in a concentration of 0.5% in 26 of 35 women in the 12th to the 24th weeks of gestation and by prostaglandin with concomitant oxytocin infusion in an additional 5 patients. A second comparable group of 35 women, with gestational ages from 9 to 23 weeks, received a silastic device with a concentration of 1.0% of the prostaglandin. Twenty-six women in the second group aborted with prostaglandin alone and an additional 6 patients aborted with combined prostaglandin-oxytocin therapy. Seven patients of the 70 included in this study failed to abort with intravaginal administration of 15-ME-PGF2alpha methyl ester even with concomitant oxytocin therapy; this group included 4 patients receiving the 0.5% device and 3 patients with the 1.0% device. The mean abortion time for the 31 successful abortions with 0.5% device was 15.15 hours and multiparous patients aborted in a mean time of 9.69 hours, significantly faster than the nulliparous patients, mean time 18.49 hours. The mean abortion time for the 32 successful abortions with the 1.0% device was 15.61 hours and there was no significant difference in the mean abortion times between nulliparous and multiparous patients in this group. No significant difference could be demonstrated between the mean abortion times of patients receiving an 0.5% concentrations of prostaglandin and patients receiving a 1.0% concentration of the drug. In both groups more than 40% of the patients had aborted within 12 hours of the insertion of the prostaglandin device and more than 80% had aborted within 24 hours. The intravaginal device appeared to be effective in inducing abortion throughout the gestational ages tested in this series. Peripheral plasma levels of 15-ME-PGF2alpha were analyzed in 15 patients with the 0.5% device and 8 patients with the 1.0% device. In patients with the 0.5% device the mean plasma concentration of prostaglandin peaked 2 hours after insertion, while in patients with the 1.0% device the peak was achieved within 15 minutes of insertion followed by a rapid decline in plasma prostaglandin levels. Diarrhea was the most frequent side effect related to the intravaginal administration of the prostaglandin, and in this series the severity of this gastro-intestinal disturbance appeared to be significantly higher in patients receiving the 1.0% device.", "PMID": 968066}
{"id": "pubmed23n0032_7221", "title": "The efficacy of intravaginal 15 methyl prostaglandin F2alpha methyl ester in first and second trimester abortion.", "content": "Fifteen methyl prostaglandin F2alpha methyl ester prepared within a silastic device for intravaginal administration appears to be advantageous. This study describes the technique carried out and the results observed in 50 patients. The analogue is prepared in 1.0% and 0.5% delivery systems. Twenty-four patients received the 1.0% dosage schedule and all twenty-four patients aborted. Twenty-six patients received 0.5% and twenty-three patients aborted. The coagulation millieu and other parameters were similar to that reported for other prostaglandins.", "contents": "The efficacy of intravaginal 15 methyl prostaglandin F2alpha methyl ester in first and second trimester abortion. Fifteen methyl prostaglandin F2alpha methyl ester prepared within a silastic device for intravaginal administration appears to be advantageous. This study describes the technique carried out and the results observed in 50 patients. The analogue is prepared in 1.0% and 0.5% delivery systems. Twenty-four patients received the 1.0% dosage schedule and all twenty-four patients aborted. Twenty-six patients received 0.5% and twenty-three patients aborted. The coagulation millieu and other parameters were similar to that reported for other prostaglandins.", "PMID": 968067}
{"id": "pubmed23n0032_7222", "title": "Clinical observations with a prostaglandin-containing silastic vaginal device for pregnancy termination.", "content": "A salastic vaginal device containing either 0.5% or 1.0% concentrations of 15(s)-15 methyl prostaglandin F2 alpha methyl ester was used to induce therapeutic abortion in 22 patients at 6-16 weeks gestation. The 1.0% device appeared to be an effacious and safe method with cumulative abortion rates similar to that seen with intra-amniotic and intramuscular prostaglandin administration. The 0.5% device appears less effective. Bothersome side effects of emesis and diarrhea were seen with both devices although no serious complications occurred. Uterine activity and serum levels of 15(s)-15 methyl PGF2alpha appeared to reflect the concentration of prostaglandin in the device. The use of a prostaglandin-containing silastic vaginal device appears to be a safe method of pregnancy termination which is convenient, effective, and applicable to a broad range of gestational ages.", "contents": "Clinical observations with a prostaglandin-containing silastic vaginal device for pregnancy termination. A salastic vaginal device containing either 0.5% or 1.0% concentrations of 15(s)-15 methyl prostaglandin F2 alpha methyl ester was used to induce therapeutic abortion in 22 patients at 6-16 weeks gestation. The 1.0% device appeared to be an effacious and safe method with cumulative abortion rates similar to that seen with intra-amniotic and intramuscular prostaglandin administration. The 0.5% device appears less effective. Bothersome side effects of emesis and diarrhea were seen with both devices although no serious complications occurred. Uterine activity and serum levels of 15(s)-15 methyl PGF2alpha appeared to reflect the concentration of prostaglandin in the device. The use of a prostaglandin-containing silastic vaginal device appears to be a safe method of pregnancy termination which is convenient, effective, and applicable to a broad range of gestational ages.", "PMID": 968068}
{"id": "pubmed23n0032_7223", "title": "[The exercise factor in the treatment of stutterers (author's transl)].", "content": "Speech techniques can always become automatic with exercises provided the only purpose is the initiation and formation of particular habitual speech-motor patterns such as the coordination of respiration and phonation. However, if the speech-technical measures alienate the stutterer's spontaneous speech, exercises designed to automate speech flow seem bound to fail, since the stutterer cannot fully identify himself with a manner of speech which is not the expression of himself. The anxiety inherent in some speech situations should be allayed by the speaker's accustomization to the adverse stimuli they produce. However, in the final analysis, the irrationality of speech situations defies a systematic approach. Thus, it is the relaxation method connected with the process of graded desensitization of the stutterer which is therapeutically effective and not his adaptation to particular speech situations.", "contents": "[The exercise factor in the treatment of stutterers (author's transl)]. Speech techniques can always become automatic with exercises provided the only purpose is the initiation and formation of particular habitual speech-motor patterns such as the coordination of respiration and phonation. However, if the speech-technical measures alienate the stutterer's spontaneous speech, exercises designed to automate speech flow seem bound to fail, since the stutterer cannot fully identify himself with a manner of speech which is not the expression of himself. The anxiety inherent in some speech situations should be allayed by the speaker's accustomization to the adverse stimuli they produce. However, in the final analysis, the irrationality of speech situations defies a systematic approach. Thus, it is the relaxation method connected with the process of graded desensitization of the stutterer which is therapeutically effective and not his adaptation to particular speech situations.", "PMID": 968160}
{"id": "pubmed23n0032_7224", "title": "Identification of glucuronide metabolites of d- and 1-methadone in bile from the isolated perfused rat liver.", "content": "Three hydroxylated metabolites of methadone, 2-ethyl-3-(dihydroxyphenyl)-5-methyl-3-phenylpyrroline (di-HO-EMDP), 2-ethyl-3-(hydroxy-methoxy-phenyl)-5-methyl-3-phenylpyrroline (HO-MeO-EMDP) and 2-ethyl-3-(hydroxy-phenyl)-5-methyl-3-phenylpyrroline (HO-EMDP) were excreted as O-glucuronide conjugates in bile from the isolated perfused rat liver following the addition of either d- or 1-methadone to the perfusate. These glucuronide metabolites were identified as intact molecules by combined gas chromatographic-mass spectrometric (GC-MS) analysis of their permethylated derivatives.", "contents": "Identification of glucuronide metabolites of d- and 1-methadone in bile from the isolated perfused rat liver. Three hydroxylated metabolites of methadone, 2-ethyl-3-(dihydroxyphenyl)-5-methyl-3-phenylpyrroline (di-HO-EMDP), 2-ethyl-3-(hydroxy-methoxy-phenyl)-5-methyl-3-phenylpyrroline (HO-MeO-EMDP) and 2-ethyl-3-(hydroxy-phenyl)-5-methyl-3-phenylpyrroline (HO-EMDP) were excreted as O-glucuronide conjugates in bile from the isolated perfused rat liver following the addition of either d- or 1-methadone to the perfusate. These glucuronide metabolites were identified as intact molecules by combined gas chromatographic-mass spectrometric (GC-MS) analysis of their permethylated derivatives.", "PMID": 968169}
{"id": "pubmed23n0032_7225", "title": "Effect of oxygen toxicity and nitric acid-induced lung damage on drug absorption from the rat lung.", "content": "To investigate the effect of pulmonary oxygen toxicity and nitric acid-induced lung damage on absorption of drugs from the lung, rats were either exposed continuously to approximately 100% oxygen or given an intratracheal injection of 1% nitric acid solution (0.15 ml), and rates of drug absorption from damaged and control lungs were compared after various times. To measure pulmonary absorption rates, 0.1 ml of drug solution (0.1-10 mM) was administered through a tight-fitting tracheal cannula to anesthetized animals, and, after various times, lungs were assayed for unabsorbed drug. Drugs investigated were procaine amide ethobromide, p-aminohippuric acid, procaine amide and mannitol-14C. Rates of drug absorption were increased 1.1-1.2 fold after 48-54 hrs of continuous oxygen exposure and 1.3-1.6 fold at 1-4 days after nitric acid treatment. The results suggest that both types of lung damage increase the porosity of the absorbing membrane, and that nitric acid damage also alters the integrity of lipoid regions of the membrane.", "contents": "Effect of oxygen toxicity and nitric acid-induced lung damage on drug absorption from the rat lung. To investigate the effect of pulmonary oxygen toxicity and nitric acid-induced lung damage on absorption of drugs from the lung, rats were either exposed continuously to approximately 100% oxygen or given an intratracheal injection of 1% nitric acid solution (0.15 ml), and rates of drug absorption from damaged and control lungs were compared after various times. To measure pulmonary absorption rates, 0.1 ml of drug solution (0.1-10 mM) was administered through a tight-fitting tracheal cannula to anesthetized animals, and, after various times, lungs were assayed for unabsorbed drug. Drugs investigated were procaine amide ethobromide, p-aminohippuric acid, procaine amide and mannitol-14C. Rates of drug absorption were increased 1.1-1.2 fold after 48-54 hrs of continuous oxygen exposure and 1.3-1.6 fold at 1-4 days after nitric acid treatment. The results suggest that both types of lung damage increase the porosity of the absorbing membrane, and that nitric acid damage also alters the integrity of lipoid regions of the membrane.", "PMID": 968170}
{"id": "pubmed23n0032_7226", "title": "Metabolism of aflatoxin B1 to aflatoxins Q1, M1 and P1 by mouse and rat.", "content": "We have used a microtechnique to study (a) the metabolism of aflatoxin (AF)B1 by rat and mouse, and (b) the effect of phenobarbital treatment in vivo on the in vitro metabolism of AFB1 by hepatic microsomes from rat and mouse. The results indicate and AFP1, the O-demethylated product of AFB1, is a major metabolite produced by the mouse. Although it is detectable in rat, the amount produced is negligible and was calculated to be at least 10 times less than that produced by the mouse. Using several microincubations, AFP1 was prepared in sufficient quantities to verify its identity by UV spectroscopy and by thin layer chromatography against an authentic standard in six different solvent systems. Phenobarbital pretreatment resulted in an enhancement in the total metabolism of AFB1 as well as in the formation of AFM1, AFQ1 and AFP1.", "contents": "Metabolism of aflatoxin B1 to aflatoxins Q1, M1 and P1 by mouse and rat. We have used a microtechnique to study (a) the metabolism of aflatoxin (AF)B1 by rat and mouse, and (b) the effect of phenobarbital treatment in vivo on the in vitro metabolism of AFB1 by hepatic microsomes from rat and mouse. The results indicate and AFP1, the O-demethylated product of AFB1, is a major metabolite produced by the mouse. Although it is detectable in rat, the amount produced is negligible and was calculated to be at least 10 times less than that produced by the mouse. Using several microincubations, AFP1 was prepared in sufficient quantities to verify its identity by UV spectroscopy and by thin layer chromatography against an authentic standard in six different solvent systems. Phenobarbital pretreatment resulted in an enhancement in the total metabolism of AFB1 as well as in the formation of AFM1, AFQ1 and AFP1.", "PMID": 968171}
{"id": "pubmed23n0032_7227", "title": "Bromobenzene hepatotoxicity in lead pretreated rats.", "content": "We have studied the levels of hepatic GSH in animals intoxicated with bromobenzene. A marked decrease of hepatic GSH was observed in poisoned rats. Such decrease was prevented by previous administration of lead nitrate, an inhibitor of DMES. In the rats treated with lead nitrate alone a rise in hepatic GSH was observed. Such protection was further observed by histological examination. The mechanism of protection exerted by lead nitrate against bromobenzene intoxication is discussed.", "contents": "Bromobenzene hepatotoxicity in lead pretreated rats. We have studied the levels of hepatic GSH in animals intoxicated with bromobenzene. A marked decrease of hepatic GSH was observed in poisoned rats. Such decrease was prevented by previous administration of lead nitrate, an inhibitor of DMES. In the rats treated with lead nitrate alone a rise in hepatic GSH was observed. Such protection was further observed by histological examination. The mechanism of protection exerted by lead nitrate against bromobenzene intoxication is discussed.", "PMID": 968172}
{"id": "pubmed23n0032_7228", "title": "Disparate effects of diethylstillbestrol and prednisolone on enzyme efflux from heart and skeletal muscle.", "content": "We have previously shown that diethylstilbestrol (DES) almost always, and prednisolone (Pr) less frequently, lowered the high serum enzyme activities in Duchenne's muscular dystrophy (DMD). In experimental studies, it was shown that pretreatment of mice with each of these agents lowered enzyme efflux from isolated skeletal muscle incubated in vitro, but efflux was augmented by higher doses of Pr. This suggested that these agents may influence skeletal muscle enzyme efflux in man also, producing the effects noted in DMD. The present studies were undertaken to assess the effect on enzyme efflux from skeletal muscle and heart that these two agents would exert when given in combination. Four groups of mice (14/group) were injected with saline, 250 mug DES, 35 mug Pr, or 250 mug DES plus 35 mug Pr in saline every other day for 22 days. The left gastroecnemius and heart were isolated from animals of each group, and placed in separate tubes containing incubation medium at 25 degrees C. The efflux of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) which issued from each organ was determined over a 5 hour period. In the doses tested, it was found that: 1) DES selectively reduced enzyme efflux from skeletal muscle, but had no effect on enzyme efflux from heart; 2) a Pr dose which decreased enzyme efflux from the heart, augmented efflux from the gastrocnemius; and 3) DES prevented the enhanced enzyme efflux produced by Pr. These studies indicate that these hormones, in pharmacological doses, influence enzyme efflux from muscle. This suggests, but it is not established, that these hormones also exert a similar physiological role. Finally, this experimental model appears to be useful in assessing the effects of single agents, and agents in combination, on enzyme efflux, and should be of aid in selecting appropriate agents which may be therapeutically useful in Duchenne's muscular dystrophy.", "contents": "Disparate effects of diethylstillbestrol and prednisolone on enzyme efflux from heart and skeletal muscle. We have previously shown that diethylstilbestrol (DES) almost always, and prednisolone (Pr) less frequently, lowered the high serum enzyme activities in Duchenne's muscular dystrophy (DMD). In experimental studies, it was shown that pretreatment of mice with each of these agents lowered enzyme efflux from isolated skeletal muscle incubated in vitro, but efflux was augmented by higher doses of Pr. This suggested that these agents may influence skeletal muscle enzyme efflux in man also, producing the effects noted in DMD. The present studies were undertaken to assess the effect on enzyme efflux from skeletal muscle and heart that these two agents would exert when given in combination. Four groups of mice (14/group) were injected with saline, 250 mug DES, 35 mug Pr, or 250 mug DES plus 35 mug Pr in saline every other day for 22 days. The left gastroecnemius and heart were isolated from animals of each group, and placed in separate tubes containing incubation medium at 25 degrees C. The efflux of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) which issued from each organ was determined over a 5 hour period. In the doses tested, it was found that: 1) DES selectively reduced enzyme efflux from skeletal muscle, but had no effect on enzyme efflux from heart; 2) a Pr dose which decreased enzyme efflux from the heart, augmented efflux from the gastrocnemius; and 3) DES prevented the enhanced enzyme efflux produced by Pr. These studies indicate that these hormones, in pharmacological doses, influence enzyme efflux from muscle. This suggests, but it is not established, that these hormones also exert a similar physiological role. Finally, this experimental model appears to be useful in assessing the effects of single agents, and agents in combination, on enzyme efflux, and should be of aid in selecting appropriate agents which may be therapeutically useful in Duchenne's muscular dystrophy.", "PMID": 968173}
{"id": "pubmed23n0032_7229", "title": "Serum levels of 5-hydroxyindole derivates after administration of L-5-hydroxytryptophan ethyl ester.", "content": "Serum levels of 5-hydroxytryptophan ethyl ester, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid (5-HTPE, 5-HTP, 5-HT and 5-HIAA, respectively), were measured after intravenous administration of L-5-HTPE in humans, premedicated with a peripheral decarboxylase inhibitor. Semi-automated methods for the estimation of the 5-hydroxyindole derivatives are described. Only serum levels of 5-HTP and 5-HIAA were found to be increased, indicating that the ethyl ester is rapidly hydrolyzed and that the decarboxylation of 5-HTP is in part inhibited. The levels of 5-HTP increased during the infusion, but dropped rapidly when the intravenous administration was terminated. Serum levels of 5-HIAA remained constant for at least 6 hours, although during the same period the levels of 5-HTP do change markedly. The persistently increased serum levels of 5-HIAA suggests, that this metabolite is formed from the 5-HT stored in peripheral or central tissue. Serum levels of 5-HIAA may therefore be indicative of changes of 5-HT metabolism during drug treatment. Levels of 5-HTP may be used for the estimation of the availability of the ethylester of 5-HTP.", "contents": "Serum levels of 5-hydroxyindole derivates after administration of L-5-hydroxytryptophan ethyl ester. Serum levels of 5-hydroxytryptophan ethyl ester, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid (5-HTPE, 5-HTP, 5-HT and 5-HIAA, respectively), were measured after intravenous administration of L-5-HTPE in humans, premedicated with a peripheral decarboxylase inhibitor. Semi-automated methods for the estimation of the 5-hydroxyindole derivatives are described. Only serum levels of 5-HTP and 5-HIAA were found to be increased, indicating that the ethyl ester is rapidly hydrolyzed and that the decarboxylation of 5-HTP is in part inhibited. The levels of 5-HTP increased during the infusion, but dropped rapidly when the intravenous administration was terminated. Serum levels of 5-HIAA remained constant for at least 6 hours, although during the same period the levels of 5-HTP do change markedly. The persistently increased serum levels of 5-HIAA suggests, that this metabolite is formed from the 5-HT stored in peripheral or central tissue. Serum levels of 5-HIAA may therefore be indicative of changes of 5-HT metabolism during drug treatment. Levels of 5-HTP may be used for the estimation of the availability of the ethylester of 5-HTP.", "PMID": 968174}
{"id": "pubmed23n0032_7230", "title": "Quantitative characterization of THC and ethanol interaction.", "content": "The anticonvulsant activity of ethanol, delta9-tetrahydrocannabinol and their combinations was measured in the maximal electroshock seizure test. The determined median anticonvulsant doses (ED50) of drug combinations were compared with those calculated on the assumption of additivity of doses. No significant difference was found between the determined and expected values. It is concluded that in this test ethanol and THC may interact additively.", "contents": "Quantitative characterization of THC and ethanol interaction. The anticonvulsant activity of ethanol, delta9-tetrahydrocannabinol and their combinations was measured in the maximal electroshock seizure test. The determined median anticonvulsant doses (ED50) of drug combinations were compared with those calculated on the assumption of additivity of doses. No significant difference was found between the determined and expected values. It is concluded that in this test ethanol and THC may interact additively.", "PMID": 968175}
{"id": "pubmed23n0032_7231", "title": "Synthesis and characterization of alpha-phenyl-gamma-butyrolactone, a metabolite of glutethimide, phenobarbital and primidone, in human urine.", "content": "A novel metabolite, alpha-phenyl-gamma-butyfolactone has been isolated in urine samples of patients severely intoxicated by either glutethimide, phenobarbital, or primidone. This lactone was prepared synthetically and its spectral data and chromatographic properties were compared to those data obtained from the urine samples of drug overdosed victims. The results of these comparisons confirm the presence of this lactone in human urine following the ingestion of large amounts of the parent drug.", "contents": "Synthesis and characterization of alpha-phenyl-gamma-butyrolactone, a metabolite of glutethimide, phenobarbital and primidone, in human urine. A novel metabolite, alpha-phenyl-gamma-butyfolactone has been isolated in urine samples of patients severely intoxicated by either glutethimide, phenobarbital, or primidone. This lactone was prepared synthetically and its spectral data and chromatographic properties were compared to those data obtained from the urine samples of drug overdosed victims. The results of these comparisons confirm the presence of this lactone in human urine following the ingestion of large amounts of the parent drug.", "PMID": 968177}
{"id": "pubmed23n0032_7232", "title": "Quantitation in plasma and urine of acebutolol and a major metabolite with preliminary observations on their disposition kinetics in man.", "content": "An acetyl-homologue-metabolite, present in concentrations of up to ten times those of the unchanged drug, in the plasma of patients receiving oral doses of acebutolol, is reported. Combined gas chromatography--mass spectrometry has been utilized to determine the structure of the acetyl-metabolite. This acetyl-metabolite has been measured as acebutolol by previously published non-specific methods for the determination of acebutolol in biological fluids. A specific and sensitive gas chromatographic method is described for the separate quantitation of acebutolol and its acetyl-metabolite in plasma and urine. Using our method, preliminary data on the disposition kinetics of acebutolol are presented. The difficulties in interpreting previously published pharmacokinetic data for acebutolol, based on a non-specific method of analysis, are emphasized.", "contents": "Quantitation in plasma and urine of acebutolol and a major metabolite with preliminary observations on their disposition kinetics in man. An acetyl-homologue-metabolite, present in concentrations of up to ten times those of the unchanged drug, in the plasma of patients receiving oral doses of acebutolol, is reported. Combined gas chromatography--mass spectrometry has been utilized to determine the structure of the acetyl-metabolite. This acetyl-metabolite has been measured as acebutolol by previously published non-specific methods for the determination of acebutolol in biological fluids. A specific and sensitive gas chromatographic method is described for the separate quantitation of acebutolol and its acetyl-metabolite in plasma and urine. Using our method, preliminary data on the disposition kinetics of acebutolol are presented. The difficulties in interpreting previously published pharmacokinetic data for acebutolol, based on a non-specific method of analysis, are emphasized.", "PMID": 968178}
{"id": "pubmed23n0032_7233", "title": "Dopaminergic activity of some apomorphine analogs.", "content": "The dopaminergic activity of ten apomorphine analogs was studied in rats lesioned unilaterally with 6-hydroxydopamine in the nigro-striatal system. Of these ten compounds, N,N-dimethyl-5,6-dihydroxy-2-amino-tetralin (M-7), N-methyl-5,6-dihydroxy-2-aminotetralin (M-8) and N,N-dimethyl-4,5-dihydroxy-2-aminoindan (DDAI) exhibited potent dopaminergic stimulant activity by causing the rat to turn to the unoperated side. The turning behavior of apomorphine, M-7, DDAI and d-amphetamine were antagonized by haloperidol. M-7 and DDAI also induced pecking in pigeons and their effects were also blocked by haloperidol. It is concluded that M-7, M-8 and DDAI are direct acting central dopaminergic agents.", "contents": "Dopaminergic activity of some apomorphine analogs. The dopaminergic activity of ten apomorphine analogs was studied in rats lesioned unilaterally with 6-hydroxydopamine in the nigro-striatal system. Of these ten compounds, N,N-dimethyl-5,6-dihydroxy-2-amino-tetralin (M-7), N-methyl-5,6-dihydroxy-2-aminotetralin (M-8) and N,N-dimethyl-4,5-dihydroxy-2-aminoindan (DDAI) exhibited potent dopaminergic stimulant activity by causing the rat to turn to the unoperated side. The turning behavior of apomorphine, M-7, DDAI and d-amphetamine were antagonized by haloperidol. M-7 and DDAI also induced pecking in pigeons and their effects were also blocked by haloperidol. It is concluded that M-7, M-8 and DDAI are direct acting central dopaminergic agents.", "PMID": 968179}
{"id": "pubmed23n0032_7234", "title": "How phlebotomy affects the erythrocyte metabolism of high- and low-glutathione sheep.", "content": "Erythrocyte glutathione concentration increases dramatically in low-glutathione sheep of the Finnish-Landrace breed. To determine if this phenomenon occurs in low-glutathione sheep of the Corriedale or Merino type, the packed cell volume was decreased to less than 16 by phlebotomy. Glutathione concentrations increased in high- and low-glutathione sheep. Low-glutathione sheep returned to pre-phlebotomy levels by the 18th day; high-glutathione sheep increased, then decreased below pre-phlebotomy levels for the duration of the experiment. Packed cell volumes increased linearly (1-07 per cent per day) for 19 days. The maximum reticulocyte percentage (11-4) occurred on the eight day. Some erythrocyte enzymes increased as the mean cell age decreased, but some did not change. Erythrocyte adenosine-5'-triphosphate and creatine appear to be related to the reticulocyte count.", "contents": "How phlebotomy affects the erythrocyte metabolism of high- and low-glutathione sheep. Erythrocyte glutathione concentration increases dramatically in low-glutathione sheep of the Finnish-Landrace breed. To determine if this phenomenon occurs in low-glutathione sheep of the Corriedale or Merino type, the packed cell volume was decreased to less than 16 by phlebotomy. Glutathione concentrations increased in high- and low-glutathione sheep. Low-glutathione sheep returned to pre-phlebotomy levels by the 18th day; high-glutathione sheep increased, then decreased below pre-phlebotomy levels for the duration of the experiment. Packed cell volumes increased linearly (1-07 per cent per day) for 19 days. The maximum reticulocyte percentage (11-4) occurred on the eight day. Some erythrocyte enzymes increased as the mean cell age decreased, but some did not change. Erythrocyte adenosine-5'-triphosphate and creatine appear to be related to the reticulocyte count.", "PMID": 968180}
{"id": "pubmed23n0032_7235", "title": "Immunisation against bovine tropical theileriasis (Theileria annulata infection).", "content": "Fully susceptible cross-bred calves, six to nine months of age, were immunised by tick-induced Theileria annulata infection treated with chlortetracycline at 16 mg/kg body weight for four, eight or 16 days. The infections were induced with 10 ticks (Hyalomma anatolicum anatolicum) or 30 ticks (H dromedarii). The recovered calves were tested for immunity to homologous severe challenge, 50 or 73 days after the first infection. The reaction of the calves to infections was evaluated by noting the prepatent period, symptoms, degree of anaemia, rate of parasitisation of lymphocytes and erythrocytes. It was observed that untreated calves developed acute theileriasis characterised by typical symptoms and lesions and 56 to 66 per cent mortality. The medicated calves, however, developed a mild form of the disease. Calves which recovered from treated or untreated infections were solidly resistant to subsequent severe homologous infection. Judged from the severity of anaemia in the infected calves, eight day and 16 day medication provided slightly better protection than four day medication. It was concluded that eight day medication afforded adequate protection against the severe immunising infection, and allowed the development of solid resistance to severe homologous challenge.", "contents": "Immunisation against bovine tropical theileriasis (Theileria annulata infection). Fully susceptible cross-bred calves, six to nine months of age, were immunised by tick-induced Theileria annulata infection treated with chlortetracycline at 16 mg/kg body weight for four, eight or 16 days. The infections were induced with 10 ticks (Hyalomma anatolicum anatolicum) or 30 ticks (H dromedarii). The recovered calves were tested for immunity to homologous severe challenge, 50 or 73 days after the first infection. The reaction of the calves to infections was evaluated by noting the prepatent period, symptoms, degree of anaemia, rate of parasitisation of lymphocytes and erythrocytes. It was observed that untreated calves developed acute theileriasis characterised by typical symptoms and lesions and 56 to 66 per cent mortality. The medicated calves, however, developed a mild form of the disease. Calves which recovered from treated or untreated infections were solidly resistant to subsequent severe homologous infection. Judged from the severity of anaemia in the infected calves, eight day and 16 day medication provided slightly better protection than four day medication. It was concluded that eight day medication afforded adequate protection against the severe immunising infection, and allowed the development of solid resistance to severe homologous challenge.", "PMID": 968181}
{"id": "pubmed23n0032_7236", "title": "Preferential transport into colostrum of Fc fragment derived from serum IgG1 immunoglobulin in the goat.", "content": "The selective transport of blood IgG1 into colostrum in ruminant species is not well understood. Therefore, the transport of Fc fragments isolated from serum IgG1 (Fc gamma 1) and IgG2 (Fc gamma 2) in normal and pregnant goats was studied. The animals were injected intravenously with radio-labelled 125I-Fcgamma1 and 131I-Fcgamma2 fragments. The plasma disappearance rate of both fragments showed that at parturition the Fcgamma1 fragment had a higher disappearance rate than Fcgamma2. Over 70 per cent of the inoculated dose of 125I-Fcgamma1 and only 11-12 per cent of 131I-Fcgamma2 could be recovered in the 24 h colostrum. The results indicated that structural differences between the H chains of IgG1 and IgG2 were implicated in the selective transport of IgG1 into colostrum in ruminants.", "contents": "Preferential transport into colostrum of Fc fragment derived from serum IgG1 immunoglobulin in the goat. The selective transport of blood IgG1 into colostrum in ruminant species is not well understood. Therefore, the transport of Fc fragments isolated from serum IgG1 (Fc gamma 1) and IgG2 (Fc gamma 2) in normal and pregnant goats was studied. The animals were injected intravenously with radio-labelled 125I-Fcgamma1 and 131I-Fcgamma2 fragments. The plasma disappearance rate of both fragments showed that at parturition the Fcgamma1 fragment had a higher disappearance rate than Fcgamma2. Over 70 per cent of the inoculated dose of 125I-Fcgamma1 and only 11-12 per cent of 131I-Fcgamma2 could be recovered in the 24 h colostrum. The results indicated that structural differences between the H chains of IgG1 and IgG2 were implicated in the selective transport of IgG1 into colostrum in ruminants.", "PMID": 968182}
{"id": "pubmed23n0032_7237", "title": "Morphology and classification of subdivisions of the intrahepatic vascular and biliary systems in sheep.", "content": "Tensol casts and histology were used to demonstrate sub-divisions of the portal and hepatic veins, and arterial and biliary systems. Portal and hepatic venous subdivisions were most readily identified. Based upon size, location, sequence of branching and histological characteristics a nomenclature for the sub-divisions of the portal vascular system was proposed. The names of distributing, primary, terminal, secondary (long and short) and tertiary veins were adopted to identify portal veins branches. The same criteria allowed the identification of three hepatic vein branches, central, sublobular and hepatic; the central hepatic veins and to a much lesser extent the sublobular hepatic veins drained the sinusoids. Casts of the arterial system demonstrated the arterial blood supply to the biliary system, to the sinusoids and portal vein vasa vasorum but they were of little use in identifying subdivisions. Arterial subdivision identification is possible by using accompanying portal veins as morphological markers and designating the subdivision after the vein. This approach is also applicable to the biliary system and provides a more accurate means of subdivision identification than does the broader division into ductules, intermediate and large ducts.", "contents": "Morphology and classification of subdivisions of the intrahepatic vascular and biliary systems in sheep. Tensol casts and histology were used to demonstrate sub-divisions of the portal and hepatic veins, and arterial and biliary systems. Portal and hepatic venous subdivisions were most readily identified. Based upon size, location, sequence of branching and histological characteristics a nomenclature for the sub-divisions of the portal vascular system was proposed. The names of distributing, primary, terminal, secondary (long and short) and tertiary veins were adopted to identify portal veins branches. The same criteria allowed the identification of three hepatic vein branches, central, sublobular and hepatic; the central hepatic veins and to a much lesser extent the sublobular hepatic veins drained the sinusoids. Casts of the arterial system demonstrated the arterial blood supply to the biliary system, to the sinusoids and portal vein vasa vasorum but they were of little use in identifying subdivisions. Arterial subdivision identification is possible by using accompanying portal veins as morphological markers and designating the subdivision after the vein. This approach is also applicable to the biliary system and provides a more accurate means of subdivision identification than does the broader division into ductules, intermediate and large ducts.", "PMID": 968183}
{"id": "pubmed23n0032_7238", "title": "Passive immunity in the lamb. Effects of a second feed of colostrum on antibody absorption from the first feed.", "content": "Lambs were taken from their mothers before sucking and fed pooled colostrum (30 g/kg body weight), containing added antibody to egg albumin, 1 h after birth. At 4 h after feeding there was a two-fold range in immunoglobulin (IgG) concentration and a sixteen-fold range in anti-egg albumin activity in their sera. The coefficient of correlation between the IgG and antibody concentrations was significant but low (r = 0.445, P less than 0.02). When a second feed of colostrum without added antibody was given 6 h after the first, both the IgG and the antibody concentrations increased significantly, indicating that there had been increased absorption from the first feed, rather than absorption from the second. The increases in IgG had a significant negative correlation with the IgG concentrations before the second feed (r = 0.760, P less than 0.001). Sera from control lambs fed only untreated colostrum gave only trace, probably non-specific, antibody reactions. The results suggest that single feeds of colostrum are generally inefficient, and that the passive immune status of young lambs may be improved and made more uniform by subsequent feeds, even though these may be themselves deficient in IgG.", "contents": "Passive immunity in the lamb. Effects of a second feed of colostrum on antibody absorption from the first feed. Lambs were taken from their mothers before sucking and fed pooled colostrum (30 g/kg body weight), containing added antibody to egg albumin, 1 h after birth. At 4 h after feeding there was a two-fold range in immunoglobulin (IgG) concentration and a sixteen-fold range in anti-egg albumin activity in their sera. The coefficient of correlation between the IgG and antibody concentrations was significant but low (r = 0.445, P less than 0.02). When a second feed of colostrum without added antibody was given 6 h after the first, both the IgG and the antibody concentrations increased significantly, indicating that there had been increased absorption from the first feed, rather than absorption from the second. The increases in IgG had a significant negative correlation with the IgG concentrations before the second feed (r = 0.760, P less than 0.001). Sera from control lambs fed only untreated colostrum gave only trace, probably non-specific, antibody reactions. The results suggest that single feeds of colostrum are generally inefficient, and that the passive immune status of young lambs may be improved and made more uniform by subsequent feeds, even though these may be themselves deficient in IgG.", "PMID": 968184}
{"id": "pubmed23n0032_7239", "title": "The rate of rise of intraventricular pressure as an index of myocardial contractility in conscious and anaesthetised ponies.", "content": "Measurements of the rate of rise of left ventricular blood pressure (dP/dt) have been made in conscious and anaesthetised ponies. Concurrent measurements of heart rate, mean arterial pressure and left ventricular pressure were also made in order to assess their relationship to values of dP/dt. Thiopentone-halothane and thiopentone-ether anaesthesia reduced the maximal rate of rise of intraventricular pressure (dP/dt max) from conscious control levels. After correcting for variations in the loading conditions of the ventricle, the depressant effect of halothane was still apparent, but the action of ether was not. It was concluded that the negative inotropic effect of halothane in the pony was greater than that of ether, possibly because a compensatory sympathoadrenal stimulation occurred during ether but not during halothane anaesthesia.", "contents": "The rate of rise of intraventricular pressure as an index of myocardial contractility in conscious and anaesthetised ponies. Measurements of the rate of rise of left ventricular blood pressure (dP/dt) have been made in conscious and anaesthetised ponies. Concurrent measurements of heart rate, mean arterial pressure and left ventricular pressure were also made in order to assess their relationship to values of dP/dt. Thiopentone-halothane and thiopentone-ether anaesthesia reduced the maximal rate of rise of intraventricular pressure (dP/dt max) from conscious control levels. After correcting for variations in the loading conditions of the ventricle, the depressant effect of halothane was still apparent, but the action of ether was not. It was concluded that the negative inotropic effect of halothane in the pony was greater than that of ether, possibly because a compensatory sympathoadrenal stimulation occurred during ether but not during halothane anaesthesia.", "PMID": 968185}
{"id": "pubmed23n0032_7240", "title": "A study of the complement fixation test in Salmonella dublin infection.", "content": "The complement fixation test was evaluated in a study of Salmonella dublin infection in cattle. In calves vaccinated with a live S dublin vaccine at three days of age, complement fixing (CF) antibodies were not detected in colostrum-fed calves although CF antibodies were detected at low titre in colostrum-deprived calves. In calves vaccinated at three months of age and in experimentally infected cows, CF antibodies persisted for three to six months but serum agglutinin (SA) antibodies persisted at diagnostic titres for longer periods. When serum samples from outbreaks of S dublin infection were examined, the CF results were in agreement with the SAT in the majority of samples obtained from adults and calves suffering from enteric disease. In samples from cases of S dublin abortion, a number of samples had negative CF titres although the SA titres were indicative of infection. Non-specific CF reactions occurred in serum samples from cattle infected with salmonella serotypes other than S dublin.", "contents": "A study of the complement fixation test in Salmonella dublin infection. The complement fixation test was evaluated in a study of Salmonella dublin infection in cattle. In calves vaccinated with a live S dublin vaccine at three days of age, complement fixing (CF) antibodies were not detected in colostrum-fed calves although CF antibodies were detected at low titre in colostrum-deprived calves. In calves vaccinated at three months of age and in experimentally infected cows, CF antibodies persisted for three to six months but serum agglutinin (SA) antibodies persisted at diagnostic titres for longer periods. When serum samples from outbreaks of S dublin infection were examined, the CF results were in agreement with the SAT in the majority of samples obtained from adults and calves suffering from enteric disease. In samples from cases of S dublin abortion, a number of samples had negative CF titres although the SA titres were indicative of infection. Non-specific CF reactions occurred in serum samples from cattle infected with salmonella serotypes other than S dublin.", "PMID": 968186}
{"id": "pubmed23n0032_7241", "title": "Characterisation of an epizootic haemorrhagic disease virus.", "content": "A high titre of epizootic haemorrhagic disease (EHD) virus was obtained from roller bottles of African green monkey kidney (Vero) cells. Cell associated EHD virus was purified by use of Genetron-113 and sonication. Purified viral particles were probably icosahedral with a diameter of 58 nm and contained capsomeres which appeared to be composed of five and six subunit structures. Phenol extracted nucleic acid from purified EHD virus when treated with orcinol or diphenylamine gave colour reactions and ultraviolet absorption spectrums characteristic for ribonucleic acid (RNA). EHD viral RNA had a melting temperature of 86 degrees C in 0.015 M NaCl-0.0015 M sodium citrate. RNA from EHD virus did not react with formaldehyde, but after it was heated at 102 degrees C for 10 min hyperchromicity was observed. Electrophoresis of EHD-RNA in 5 per cent polyacrylamide gels showed the genome to be composed of at least five different segments.", "contents": "Characterisation of an epizootic haemorrhagic disease virus. A high titre of epizootic haemorrhagic disease (EHD) virus was obtained from roller bottles of African green monkey kidney (Vero) cells. Cell associated EHD virus was purified by use of Genetron-113 and sonication. Purified viral particles were probably icosahedral with a diameter of 58 nm and contained capsomeres which appeared to be composed of five and six subunit structures. Phenol extracted nucleic acid from purified EHD virus when treated with orcinol or diphenylamine gave colour reactions and ultraviolet absorption spectrums characteristic for ribonucleic acid (RNA). EHD viral RNA had a melting temperature of 86 degrees C in 0.015 M NaCl-0.0015 M sodium citrate. RNA from EHD virus did not react with formaldehyde, but after it was heated at 102 degrees C for 10 min hyperchromicity was observed. Electrophoresis of EHD-RNA in 5 per cent polyacrylamide gels showed the genome to be composed of at least five different segments.", "PMID": 968187}
{"id": "pubmed23n0032_7242", "title": "Factors regulating tapeworm populations: estimations of the infection pressure and index of clustering from Taenia hydatigena before and after the removal of infected dogs.", "content": "A pasture was prepared so that an equilibrium of fresh and ageing eggs of Taenia hydatigena was present. This was done by placing dogs with patent infections on it for six months. Five months after their introduction, groups of lambs were grazed there for two, four, eight, 16 or 32 days. The dogs were then removed. Three and six months later, further groups of sheep were grazed there for a similar period. The infection pressures and the indices of clustering of the eggs were calculated by an examination of the lambs for cysts at autopsy. When the dogs were present, the daily rate of infection was about 60 per cent of the flock. This was reduced to 6.5 per cent then to 3.5 per cent within three and six months respectively after their removal. Very high cyst counts were obtained in some animals and the indices of dispersion diverged from a Poisson model during the period when the lambs were maintained on the pasture in the presence of the dogs. There was a trend for a reduction in the mean cyst counts and the index of dispersion tended to conform more closely to a Poisson model the longer the dogs were excluded. There was also a corresponding reduction in the survival rate of the cysts. It was suggested that an asymptote of less than 100 per cent in the prevalence rate may be accounted for by the acquisition of resistance without the persistence of infection and this was induced in some animals by the initial ingestion of ageing organisms which gradually formed the predominant type after the removal of the dogs from the pasture.", "contents": "Factors regulating tapeworm populations: estimations of the infection pressure and index of clustering from Taenia hydatigena before and after the removal of infected dogs. A pasture was prepared so that an equilibrium of fresh and ageing eggs of Taenia hydatigena was present. This was done by placing dogs with patent infections on it for six months. Five months after their introduction, groups of lambs were grazed there for two, four, eight, 16 or 32 days. The dogs were then removed. Three and six months later, further groups of sheep were grazed there for a similar period. The infection pressures and the indices of clustering of the eggs were calculated by an examination of the lambs for cysts at autopsy. When the dogs were present, the daily rate of infection was about 60 per cent of the flock. This was reduced to 6.5 per cent then to 3.5 per cent within three and six months respectively after their removal. Very high cyst counts were obtained in some animals and the indices of dispersion diverged from a Poisson model during the period when the lambs were maintained on the pasture in the presence of the dogs. There was a trend for a reduction in the mean cyst counts and the index of dispersion tended to conform more closely to a Poisson model the longer the dogs were excluded. There was also a corresponding reduction in the survival rate of the cysts. It was suggested that an asymptote of less than 100 per cent in the prevalence rate may be accounted for by the acquisition of resistance without the persistence of infection and this was induced in some animals by the initial ingestion of ageing organisms which gradually formed the predominant type after the removal of the dogs from the pasture.", "PMID": 968188}
{"id": "pubmed23n0032_7243", "title": "Factors regulating tapeworm populations: estimations of the build-up and dispersion patterns of eggs after the introduction of dogs infected with Taenia hydatigena.", "content": "The build-up of the eggs of Taenia hydatigena was measured on a pasture following the introduction of infected dogs. Susceptible lambs grazed on the pasture beforehand showed that only small numbers of randomly dispersed infective eggs were present. Two dogs each with four patent T hydatigena were chained to kennels placed in the middle of the pasture. The build-up of eggs was determined by grazing further tapeworm-free lambs there for 10-day periods. High cyst counts were obtained in some of these lambs grazed within 10 days of the introduction of the infected dogs. The mean cyst counts remained at a similar level during the 85-day investigation. This indicated that at least part of the pasture became virtually saturated with eggs shortly after the dogs were introduced and remained so while the dogs were there. Some animal management practices that favour this 'epidemic' pattern and lead to a 'cysticercosis storm' are discussed.", "contents": "Factors regulating tapeworm populations: estimations of the build-up and dispersion patterns of eggs after the introduction of dogs infected with Taenia hydatigena. The build-up of the eggs of Taenia hydatigena was measured on a pasture following the introduction of infected dogs. Susceptible lambs grazed on the pasture beforehand showed that only small numbers of randomly dispersed infective eggs were present. Two dogs each with four patent T hydatigena were chained to kennels placed in the middle of the pasture. The build-up of eggs was determined by grazing further tapeworm-free lambs there for 10-day periods. High cyst counts were obtained in some of these lambs grazed within 10 days of the introduction of the infected dogs. The mean cyst counts remained at a similar level during the 85-day investigation. This indicated that at least part of the pasture became virtually saturated with eggs shortly after the dogs were introduced and remained so while the dogs were there. Some animal management practices that favour this 'epidemic' pattern and lead to a 'cysticercosis storm' are discussed.", "PMID": 968189}
{"id": "pubmed23n0032_7244", "title": "Factors regulating tapeworm populations: the changing opportunities of lambs for ingesting the eggs of Taenia hydatigena.", "content": "Some intrinsic factors that may modify the transmission of cysticercosis caused by Taenia hydatigena in lambs have been assessed and the age at which the infective pattern should no longer be modified by the grazing-learning process and by colostrum-derived antibody, estimated. Lambs of 0-1, 2-3, 5-6, 8-9, 11-12 weeks of age, were placed for one week on a pasture experimentally contaminated with eggs of T hydatigena. The lambs were allowed to suck their mothers from birth. About half the lambs up to three weeks of age were free from larval tapeworms at autopsy; the rest had only a small number of cysts. Some of the lambs grazed on the pasture at or after five weeks of age, became heavily infected. The light infections in the very young lambs could have resulted from either a limited contact with eggs or from passively transferred antibody or, more likely, from a combination of these factors. The high cyst counts observed in some of the older lambs were consistent with the ingestion of eggs in clusters. The rise in the total cyst counts was concomitant with increasing pasture intake. The higher survival rates for the cysts found in the older rather than the younger animals were compatible with the waning before five weeks of age of colostrum-derived antibody.", "contents": "Factors regulating tapeworm populations: the changing opportunities of lambs for ingesting the eggs of Taenia hydatigena. Some intrinsic factors that may modify the transmission of cysticercosis caused by Taenia hydatigena in lambs have been assessed and the age at which the infective pattern should no longer be modified by the grazing-learning process and by colostrum-derived antibody, estimated. Lambs of 0-1, 2-3, 5-6, 8-9, 11-12 weeks of age, were placed for one week on a pasture experimentally contaminated with eggs of T hydatigena. The lambs were allowed to suck their mothers from birth. About half the lambs up to three weeks of age were free from larval tapeworms at autopsy; the rest had only a small number of cysts. Some of the lambs grazed on the pasture at or after five weeks of age, became heavily infected. The light infections in the very young lambs could have resulted from either a limited contact with eggs or from passively transferred antibody or, more likely, from a combination of these factors. The high cyst counts observed in some of the older lambs were consistent with the ingestion of eggs in clusters. The rise in the total cyst counts was concomitant with increasing pasture intake. The higher survival rates for the cysts found in the older rather than the younger animals were compatible with the waning before five weeks of age of colostrum-derived antibody.", "PMID": 968190}
{"id": "pubmed23n0032_7245", "title": "Induction of pneumonia in gnotobiotic calves following inoculation of Mycoplasma dispar and ureaplasmas (T-mycoplasmas).", "content": "Cloned cultures of Mycoplasma dispar and of Ureaplasma sp were inoculated endobronchially into gnotobiotic calves. The resulting pneumonia was estimated to involve 2 to 10 per cent of the lung although no clinical signs were observed. The histopathological lesions consisted of infiltration with mononuclear cells, cuffing (peribronchiolar lympho-reticular hyperplasia), catarrhal bronchiolitis and atelectasis. The results indicate that both M dispar and Ureaplasma sp are capable of causing sub-clinical cuffing pneumonia in calves.", "contents": "Induction of pneumonia in gnotobiotic calves following inoculation of Mycoplasma dispar and ureaplasmas (T-mycoplasmas). Cloned cultures of Mycoplasma dispar and of Ureaplasma sp were inoculated endobronchially into gnotobiotic calves. The resulting pneumonia was estimated to involve 2 to 10 per cent of the lung although no clinical signs were observed. The histopathological lesions consisted of infiltration with mononuclear cells, cuffing (peribronchiolar lympho-reticular hyperplasia), catarrhal bronchiolitis and atelectasis. The results indicate that both M dispar and Ureaplasma sp are capable of causing sub-clinical cuffing pneumonia in calves.", "PMID": 968191}
{"id": "pubmed23n0032_7246", "title": "The anthelmintic efficacy of fenbendazole against thiabendazole-resistant strains of Haemonchus contortus and Trichostrongylus colubriformis in sheep.", "content": "The anthelmintic efficacy of fenbendazole (methyl 5-(phenylthio)-2-benzimidazole carbamate) was tested in sheep against standardised strains of Hcaemonchus contortus and Trichostrongylus colubriformis, known to be resistant to thiabendazole (LD90 for thiabendazole against H ontortus was 200 mg/kg bodyweight and against T colubriformis was 150 mg/kg). Fenbendazole at dose rates of 5, 10 and 20 mg/kg per os reduced total worm counts in H contortus infected sheep by 66, 90 and 100 per cent respectively, with similar reductions recorded for worm egg outputs. For the thiabendazole resistant strain of T colubriformis, fenbendazole reduced total worm counts in infected sheep by 4, 44 (40-48), 79 (75-83), 96 and 100 per cent at dose rates of 5, 10, 20, 40 and 80 mg/kg per os. Significant suppression of worm-egg production by thiabendazole resistant T colubriformis was obtained with fenbendazole at dose levels of 5 mg/kg and above. The implications of these results are discussed in the light of the increasing occurrence of strains of trichostrongylid nematodes resistant to currently available benzimidazole anthelmintics.", "contents": "The anthelmintic efficacy of fenbendazole against thiabendazole-resistant strains of Haemonchus contortus and Trichostrongylus colubriformis in sheep. The anthelmintic efficacy of fenbendazole (methyl 5-(phenylthio)-2-benzimidazole carbamate) was tested in sheep against standardised strains of Hcaemonchus contortus and Trichostrongylus colubriformis, known to be resistant to thiabendazole (LD90 for thiabendazole against H ontortus was 200 mg/kg bodyweight and against T colubriformis was 150 mg/kg). Fenbendazole at dose rates of 5, 10 and 20 mg/kg per os reduced total worm counts in H contortus infected sheep by 66, 90 and 100 per cent respectively, with similar reductions recorded for worm egg outputs. For the thiabendazole resistant strain of T colubriformis, fenbendazole reduced total worm counts in infected sheep by 4, 44 (40-48), 79 (75-83), 96 and 100 per cent at dose rates of 5, 10, 20, 40 and 80 mg/kg per os. Significant suppression of worm-egg production by thiabendazole resistant T colubriformis was obtained with fenbendazole at dose levels of 5 mg/kg and above. The implications of these results are discussed in the light of the increasing occurrence of strains of trichostrongylid nematodes resistant to currently available benzimidazole anthelmintics.", "PMID": 968192}
{"id": "pubmed23n0032_7247", "title": "Studies on the epidemiology of Uncinaria stenocephala infections in British greyhounds: seasonal availability of larvae on grass runs.", "content": "The numbers of Uncinaria stenocephala larvae on the grass in a small paddock used continually for exercising greyhounds followed the seasonal pattern associated with gastro-intestinal nematodes of ruminants, pigs and horses in Britain. Contamination persisted throughout the winter months but near-zero values were recorded in the early summer, followed by a rapid rise in mid-July to a peak level in September.", "contents": "Studies on the epidemiology of Uncinaria stenocephala infections in British greyhounds: seasonal availability of larvae on grass runs. The numbers of Uncinaria stenocephala larvae on the grass in a small paddock used continually for exercising greyhounds followed the seasonal pattern associated with gastro-intestinal nematodes of ruminants, pigs and horses in Britain. Contamination persisted throughout the winter months but near-zero values were recorded in the early summer, followed by a rapid rise in mid-July to a peak level in September.", "PMID": 968193}
{"id": "pubmed23n0032_7248", "title": "Detection of antibiotic residues in calf tissues.", "content": "Groups of calves were injected with penicillin, ampicillin or oxytetracycline and a variety of samples were taken from the calves when slaughtered and tested for the presence of antibiotic residues by microbiological assay. Diaphragm and urine samples were found to be suitable for measuring detectable levels of antibiotics in calves given therapeutic injections.", "contents": "Detection of antibiotic residues in calf tissues. Groups of calves were injected with penicillin, ampicillin or oxytetracycline and a variety of samples were taken from the calves when slaughtered and tested for the presence of antibiotic residues by microbiological assay. Diaphragm and urine samples were found to be suitable for measuring detectable levels of antibiotics in calves given therapeutic injections.", "PMID": 968194}
{"id": "pubmed23n0032_7249", "title": "Auto-antibodies in ovine fascioliasis.", "content": "In sheep, low complement fixation (CP) and passive haemagglutinating (PH) auto-antibody titres increased after primary and challenge infections with F hepatica, possibly to aid removal of tissue debris. Although the CF auto-antibodies were neither organ nor species specific and cross precipitating activity could be detected between sheep liver, kidney, lung and cattle and human livers with heterologous hyperimmune whole sheep liver anti-serum, sheep mitochondria do appear to contain an organ and species specific pH antigen.", "contents": "Auto-antibodies in ovine fascioliasis. In sheep, low complement fixation (CP) and passive haemagglutinating (PH) auto-antibody titres increased after primary and challenge infections with F hepatica, possibly to aid removal of tissue debris. Although the CF auto-antibodies were neither organ nor species specific and cross precipitating activity could be detected between sheep liver, kidney, lung and cattle and human livers with heterologous hyperimmune whole sheep liver anti-serum, sheep mitochondria do appear to contain an organ and species specific pH antigen.", "PMID": 968195}
{"id": "pubmed23n0032_7250", "title": "In vitro maintenance of juvenile Fasciola gigantica and their use to establish infections in mice.", "content": "Studies on the growth of juvenile Fasciola gigantica in vitro on tissue culture feeder layers and their subsequent infectivity for mice have indicated that although no significant growth occurs in vitro the juveniles are not damaged physiologically for at least six weeks. Thus the in vitro system may be used with confidence in studies of the physiology of newly excysted flukes. Significantly more flukes became established in mice from an intraperitoneal injection of juveniles than from an oral infection of metacercariae. This may be due to inappropriate physicochemical conditions for excystment in the gut of the mouse. Early growth of flukes in mice occurred at a similar rate to that in cattle, so the mouse is probably a satisfactory model for studies on migration and establishment of F gigantica.", "contents": "In vitro maintenance of juvenile Fasciola gigantica and their use to establish infections in mice. Studies on the growth of juvenile Fasciola gigantica in vitro on tissue culture feeder layers and their subsequent infectivity for mice have indicated that although no significant growth occurs in vitro the juveniles are not damaged physiologically for at least six weeks. Thus the in vitro system may be used with confidence in studies of the physiology of newly excysted flukes. Significantly more flukes became established in mice from an intraperitoneal injection of juveniles than from an oral infection of metacercariae. This may be due to inappropriate physicochemical conditions for excystment in the gut of the mouse. Early growth of flukes in mice occurred at a similar rate to that in cattle, so the mouse is probably a satisfactory model for studies on migration and establishment of F gigantica.", "PMID": 968196}
{"id": "pubmed23n0032_7251", "title": "Functional recovery from spinal cord trauma following dexamethazone and chlorpromazine therapy in dogs.", "content": "The spinal cords of 20 normal dogs were exposed via dorsal laminectomies and damaged with a known impact force. Ten dogs were treated with dexamethazone and 10 dogs with chlorpromazine for five days after surgery. The results showed that neither drug had a significantly beneficial effect on the functional recovery of the spinal cords, when compared to a control group of similarly traumatised dogs.", "contents": "Functional recovery from spinal cord trauma following dexamethazone and chlorpromazine therapy in dogs. The spinal cords of 20 normal dogs were exposed via dorsal laminectomies and damaged with a known impact force. Ten dogs were treated with dexamethazone and 10 dogs with chlorpromazine for five days after surgery. The results showed that neither drug had a significantly beneficial effect on the functional recovery of the spinal cords, when compared to a control group of similarly traumatised dogs.", "PMID": 968197}
{"id": "pubmed23n0032_7252", "title": "The lipid composition of cattle sebaceous glands: a comparison with skin surface lipid.", "content": "The lipid composition of isolated sebaceous glands from Ayrshire calves was compared with that of freshly secreted skin surface lipid. The sebaceous gland contained a higher proportion of phospholipid and unesterified fatty acid and a lower proportion of triglyceride; otherwise there was little difference in lipid composition between samples from the gland and the skin surface.", "contents": "The lipid composition of cattle sebaceous glands: a comparison with skin surface lipid. The lipid composition of isolated sebaceous glands from Ayrshire calves was compared with that of freshly secreted skin surface lipid. The sebaceous gland contained a higher proportion of phospholipid and unesterified fatty acid and a lower proportion of triglyceride; otherwise there was little difference in lipid composition between samples from the gland and the skin surface.", "PMID": 968198}
{"id": "pubmed23n0032_7253", "title": "Effect of papain-induced emphysema on the distrubtion of pleural surface pressure.", "content": "Distribution of transpulmonary pressure (Ptp) at FRC, during spontaneous breathing, and during inflation of the relaxed respiratory system was studied in the supine, lateral and head-up postures after papain had been injected intratracheally into dogs. Functional and morphological changes of the lung resembling those of panlobular emphysema occurred in papain-treated dogs. In all postures the relationship between lung height and Ptp at FRC was steeper and shifted to the left of that obtaining for normal dogs. During artifical inflation changes of Ptp were larger in the dependent than in the upper lung and the vertical gradient of Ptp eventually disappeared, as observed in normal dogs. During spontaneous breathing changes of Ptp were uniform in the horizontal postures, but larger over the upper regions in the head-up posture; whereas they are uniform in all postures in normal dogs. From local Ptp values and lung P-V curve, distribution of specific lung volumes at FRC and ventilation during spontaneous breathing were assessed for both normal and papain-treated head-up dogs: the results agreed with those obtained using radioactive gases in normal and in elderly man or emphysematous subjects with no apparently localized lesions.", "contents": "Effect of papain-induced emphysema on the distrubtion of pleural surface pressure. Distribution of transpulmonary pressure (Ptp) at FRC, during spontaneous breathing, and during inflation of the relaxed respiratory system was studied in the supine, lateral and head-up postures after papain had been injected intratracheally into dogs. Functional and morphological changes of the lung resembling those of panlobular emphysema occurred in papain-treated dogs. In all postures the relationship between lung height and Ptp at FRC was steeper and shifted to the left of that obtaining for normal dogs. During artifical inflation changes of Ptp were larger in the dependent than in the upper lung and the vertical gradient of Ptp eventually disappeared, as observed in normal dogs. During spontaneous breathing changes of Ptp were uniform in the horizontal postures, but larger over the upper regions in the head-up posture; whereas they are uniform in all postures in normal dogs. From local Ptp values and lung P-V curve, distribution of specific lung volumes at FRC and ventilation during spontaneous breathing were assessed for both normal and papain-treated head-up dogs: the results agreed with those obtained using radioactive gases in normal and in elderly man or emphysematous subjects with no apparently localized lesions.", "PMID": 968199}
{"id": "pubmed23n0032_7254", "title": "Fluid flow in the human foetal lung: a theoretical model.", "content": "A theoretical model is developed to predict pressure changes and velocity profiles within the foetal lung during its sporadic bursts of activity. Because of the small volume flow rates and relatively high frequencies, the linearised, unidirectional Navier-Stokes equations are used to calculate these values. About 70% of the pressure drop occurs in the first four generations and is an order of magnitude higher than the equivalent Poiseuille pressure drop. Velocity profiles, pressure falls within each generation together with the total pressure drop at different times during the cycle are illustrated.", "contents": "Fluid flow in the human foetal lung: a theoretical model. A theoretical model is developed to predict pressure changes and velocity profiles within the foetal lung during its sporadic bursts of activity. Because of the small volume flow rates and relatively high frequencies, the linearised, unidirectional Navier-Stokes equations are used to calculate these values. About 70% of the pressure drop occurs in the first four generations and is an order of magnitude higher than the equivalent Poiseuille pressure drop. Velocity profiles, pressure falls within each generation together with the total pressure drop at different times during the cycle are illustrated.", "PMID": 968200}
{"id": "pubmed23n0032_7255", "title": "The effect of limb movements on the regulation of depth and rate of breathing.", "content": "In anesthetized dogs and rabbits passive or active limb movements (1) shifted to the left the relationship between tidal volume (Vt) and inspiratory time (Ti), (2) lowered the relationship between expiratory time (Te) and Ti and decreased its slope, and (3) increased the output to the inspiratory muscles (Vt/Ti). These effects increased with increasing the frequency of movements. Similar effects were obtained after vagotomy. When the stimulus was started during expiration. Te was shorted in spite of the previous unaffected Ti, Arterial PCO2 during exercise was similar (active movements) or below (passive movements) control value. Since other chemical and physical humoral factors do not seem involved, the whole increase of ventilation should be produced by neurogenic stimuli. The time course of Te, Ti and VT/Ti at the onset and at the offset of limb movements indicates an abrupt and a slow component in the neurogenic drive. A single contraction of the limb during expiration of inspiration affected the timing and the VT/Ti of 3-8 breaths.", "contents": "The effect of limb movements on the regulation of depth and rate of breathing. In anesthetized dogs and rabbits passive or active limb movements (1) shifted to the left the relationship between tidal volume (Vt) and inspiratory time (Ti), (2) lowered the relationship between expiratory time (Te) and Ti and decreased its slope, and (3) increased the output to the inspiratory muscles (Vt/Ti). These effects increased with increasing the frequency of movements. Similar effects were obtained after vagotomy. When the stimulus was started during expiration. Te was shorted in spite of the previous unaffected Ti, Arterial PCO2 during exercise was similar (active movements) or below (passive movements) control value. Since other chemical and physical humoral factors do not seem involved, the whole increase of ventilation should be produced by neurogenic stimuli. The time course of Te, Ti and VT/Ti at the onset and at the offset of limb movements indicates an abrupt and a slow component in the neurogenic drive. A single contraction of the limb during expiration of inspiration affected the timing and the VT/Ti of 3-8 breaths.", "PMID": 968201}
{"id": "pubmed23n0032_7256", "title": "Effects of ambient pressures, He and Sf6 on O2 and CO2 transport in the avian egg.", "content": "The CO2 and O2 tnesions were determined in the air cells of 14-16 day old chicken eggs before and after transfer to a 21% O2 in He or SF6 atmosphere. In the former gas mixture the air-cell PCO2 (which reflects the arterialized blood PCO2) fell rapidly from 32 torr in air to 17 torr in He-O2 attaining a new steady state in 2-4 h. In the SF6-O2 mixture PCO2 rose from 36 torr in a similar period. A similar PCO2 increase was also observed when eggs were compressed to 2 atm of air while exposure to 0.5 atm with a 40% O2-N2 mixture decreased PCO2. Since gas transport across the eggshell is by gas phase diffusion, these findings can be explained by the changes in the diffusion coefficient of CO2 which increase in the presence of He and decrease in SF6. Furthermore, the diffusion coefficient is also inversely related to the absolute pressure. Quantitative prediction of the changes of PCO2 in the He and SF6 mixture cannot be made, since the binary diffusion coefficients are not necessarily applicable in a ternary gas mixture. However, effective diffusion coefficients in these multicomponent mixtures can be derived on the basis of the observed PCO2 changes.", "contents": "Effects of ambient pressures, He and Sf6 on O2 and CO2 transport in the avian egg. The CO2 and O2 tnesions were determined in the air cells of 14-16 day old chicken eggs before and after transfer to a 21% O2 in He or SF6 atmosphere. In the former gas mixture the air-cell PCO2 (which reflects the arterialized blood PCO2) fell rapidly from 32 torr in air to 17 torr in He-O2 attaining a new steady state in 2-4 h. In the SF6-O2 mixture PCO2 rose from 36 torr in a similar period. A similar PCO2 increase was also observed when eggs were compressed to 2 atm of air while exposure to 0.5 atm with a 40% O2-N2 mixture decreased PCO2. Since gas transport across the eggshell is by gas phase diffusion, these findings can be explained by the changes in the diffusion coefficient of CO2 which increase in the presence of He and decrease in SF6. Furthermore, the diffusion coefficient is also inversely related to the absolute pressure. Quantitative prediction of the changes of PCO2 in the He and SF6 mixture cannot be made, since the binary diffusion coefficients are not necessarily applicable in a ternary gas mixture. However, effective diffusion coefficients in these multicomponent mixtures can be derived on the basis of the observed PCO2 changes.", "PMID": 968202}
{"id": "pubmed23n0032_7257", "title": "Ternary gas diffusion - in vitro studies.", "content": "The purpose of these experiments was to compare diffusive gas movement in a two-gas system with that in a three-gas system. Gas mixtures of different compositions were placed initially on either side of a removable partition dividing a cylindrical lucite diffusion chamber, filled with 3 mm glass beads. This served to slow diffusion, minimize convective currents generated by removing the partition, and stabilize temperature within the chamber. In two-gas systems, after the partition was removed, oxygen equilibrated between the two parts of the chamber more rapidly in a helium environment than in a nitrogen environment, conforming with predictions based on binary gas laws. Results obtained with a three-gas system differed significantly from those obtained with the binary system. With 21% oxygen in belium initially in one half of the chamber and 21% oxygen in nitrogen in the other, PO2 rose transiently in the He-O2 side of the chamber. Qualitatively, similar results were obtained when the O2-N2 mixture was replaced by 100% nitrogen. Pressure in the system remained essentially constant. The possible mechanisms responsible for the PO2 rise were studied using a computer model of the system. This showed that movement of a given gas may be affected significantly by movement of other gases in the system. Hence, application of binary gas diffusion laws to systems containing more than two gases may lead to significant errors.", "contents": "Ternary gas diffusion - in vitro studies. The purpose of these experiments was to compare diffusive gas movement in a two-gas system with that in a three-gas system. Gas mixtures of different compositions were placed initially on either side of a removable partition dividing a cylindrical lucite diffusion chamber, filled with 3 mm glass beads. This served to slow diffusion, minimize convective currents generated by removing the partition, and stabilize temperature within the chamber. In two-gas systems, after the partition was removed, oxygen equilibrated between the two parts of the chamber more rapidly in a helium environment than in a nitrogen environment, conforming with predictions based on binary gas laws. Results obtained with a three-gas system differed significantly from those obtained with the binary system. With 21% oxygen in belium initially in one half of the chamber and 21% oxygen in nitrogen in the other, PO2 rose transiently in the He-O2 side of the chamber. Qualitatively, similar results were obtained when the O2-N2 mixture was replaced by 100% nitrogen. Pressure in the system remained essentially constant. The possible mechanisms responsible for the PO2 rise were studied using a computer model of the system. This showed that movement of a given gas may be affected significantly by movement of other gases in the system. Hence, application of binary gas diffusion laws to systems containing more than two gases may lead to significant errors.", "PMID": 968203}
{"id": "pubmed23n0032_7258", "title": "Airway occlusion pressures in awake and anesthetized goats.", "content": "The pressures generated by the inspiratory muscles as they contract isometrically during airway occlusion seem to be a measure of respiratory neuron efferent activity. The ventilatory and occlusion pressure responses to increasing levels of CO2 were studied in goats, awake and anesthetized, with and without inspiratory flow resistance. Hypercapnia was produced by rebreathing. Randomly, during rebreathing, inspiratory airflow was prevented on single breaths. Ventilation and pressures developed during the first 100, 200, 300 and 400 milliseconds of an inspiratory effort against a complete occlusion increased linearly with CO2 in both awake and anesthetized animals. Anesthesia reduced both the ventilatory and occlusion pressure responses to CO2. Inspiratory resistance increased occlusion pressure responses in awake goats but not in the same animals when anesthetized. Inspiratory airflow resistance seems to augment respiratory efferent activity as reflected in the pressure responses only in conscious goats. Thus the response to an inspiratory resistance seems to depend on the state of consciousness.", "contents": "Airway occlusion pressures in awake and anesthetized goats. The pressures generated by the inspiratory muscles as they contract isometrically during airway occlusion seem to be a measure of respiratory neuron efferent activity. The ventilatory and occlusion pressure responses to increasing levels of CO2 were studied in goats, awake and anesthetized, with and without inspiratory flow resistance. Hypercapnia was produced by rebreathing. Randomly, during rebreathing, inspiratory airflow was prevented on single breaths. Ventilation and pressures developed during the first 100, 200, 300 and 400 milliseconds of an inspiratory effort against a complete occlusion increased linearly with CO2 in both awake and anesthetized animals. Anesthesia reduced both the ventilatory and occlusion pressure responses to CO2. Inspiratory resistance increased occlusion pressure responses in awake goats but not in the same animals when anesthetized. Inspiratory airflow resistance seems to augment respiratory efferent activity as reflected in the pressure responses only in conscious goats. Thus the response to an inspiratory resistance seems to depend on the state of consciousness.", "PMID": 968204}
{"id": "pubmed23n0032_7259", "title": "[Technic for an inflatable and releasable balloon in neurology. Experimental study. Application in man].", "content": "We consider our method with an inflatable and releasable balloon to be trustworthy and now applicable to the treatment of carotido-cavernous fistulas; it may not be possible in all cases to pass the ballon on through the fistula and inflate it in the cavernous sinus, but it seems to us so very important to preserve the carotid flow that we think that this should be achieved whenever possible. Certain arterial aneurysms could profit from this technique. We have purposely omitted all diagnostic application of the method which serbinenko has described in his article: selective opacifcation of certain branches of the internal carotid, deliberate temporary occlusion of certain branches to enable the amount of tolerance to be assessed. All these exploratory methods which we are beginning to carry out will form the subject of a later paper.", "contents": "[Technic for an inflatable and releasable balloon in neurology. Experimental study. Application in man]. We consider our method with an inflatable and releasable balloon to be trustworthy and now applicable to the treatment of carotido-cavernous fistulas; it may not be possible in all cases to pass the ballon on through the fistula and inflate it in the cavernous sinus, but it seems to us so very important to preserve the carotid flow that we think that this should be achieved whenever possible. Certain arterial aneurysms could profit from this technique. We have purposely omitted all diagnostic application of the method which serbinenko has described in his article: selective opacifcation of certain branches of the internal carotid, deliberate temporary occlusion of certain branches to enable the amount of tolerance to be assessed. All these exploratory methods which we are beginning to carry out will form the subject of a later paper.", "PMID": 968311}
{"id": "pubmed23n0032_7260", "title": "[Striato-nigral degeneration. A propos of 2 anatomo-clinical cases].", "content": "Two sporadic cases of striato-nigral degeneration were characterized by the onset at the age of 61 of an akinetic-hypertonic syndrome and of a minimal or absent rest tremor, with a pyramidal syndrome, sphincter disorders, dysphonia, difficulty in swallowing and an unfavorable development in the space of two or three years not affected by L-Dopa. In one case, total insomnia was demonstrated by means of 3 polygraphic recordings during the night, two of them consecutive, and aprobenecide test showed a definite fall in H.V.A. and 5 H.I.A.A. in the lumbar cerebrospinal fluid. Anatomical verification showed, in one case, isolated putamino-nigral degeneration, and in the other associated with lesions of the olivo-cerebellopontine system. Much of the excess pigmentation found only in the putamen was melanotic in character and resulted perhaps from the striato-nigral degeneration, making it possible to classify this more accurately among the other multisystem degenerations, especially olivo-ponto-cerebellar atrophy and Shy-Drager syndrome", "contents": "[Striato-nigral degeneration. A propos of 2 anatomo-clinical cases]. Two sporadic cases of striato-nigral degeneration were characterized by the onset at the age of 61 of an akinetic-hypertonic syndrome and of a minimal or absent rest tremor, with a pyramidal syndrome, sphincter disorders, dysphonia, difficulty in swallowing and an unfavorable development in the space of two or three years not affected by L-Dopa. In one case, total insomnia was demonstrated by means of 3 polygraphic recordings during the night, two of them consecutive, and aprobenecide test showed a definite fall in H.V.A. and 5 H.I.A.A. in the lumbar cerebrospinal fluid. Anatomical verification showed, in one case, isolated putamino-nigral degeneration, and in the other associated with lesions of the olivo-cerebellopontine system. Much of the excess pigmentation found only in the putamen was melanotic in character and resulted perhaps from the striato-nigral degeneration, making it possible to classify this more accurately among the other multisystem degenerations, especially olivo-ponto-cerebellar atrophy and Shy-Drager syndrome", "PMID": 968312}
{"id": "pubmed23n0032_7261", "title": "[Morphological aspects (optical and ultrastructural) of hypertrophy of the olivary bulb].", "content": "The present paper is an anatomical study of 3 cases which evolved with hypertrophy of the inferior olives: a case of zoster encephalitis, a multifocal leucoencephalopathy and a post-traumatic encephalopathy. In all three cases a relationship was demonstrated between the dentate nucleus and dento-olivary pathway - a relation of topical order in some instances - and the hypertrophic lesion of the inferior olive. Examination in the electron microscope revealed in the hypertrophic olivary cell not only dystrophic lesions but also progressive intracellular phenomena, such as maintenance of the Nissl bodies, multiplication of the mitochondria and especially marked proliferation of the neuro-filaments. This shows that, at least in some of the evolutive phases, hypertrophy of the inferior olives is based upon a real hypertrophy of the olivary cell. In addition, lesions of the glial system of the hypertrophic inferior olives were noted with the presence of osmiophil degeneration whose morphology clearly differed from that of Rosenthal's fibres.", "contents": "[Morphological aspects (optical and ultrastructural) of hypertrophy of the olivary bulb]. The present paper is an anatomical study of 3 cases which evolved with hypertrophy of the inferior olives: a case of zoster encephalitis, a multifocal leucoencephalopathy and a post-traumatic encephalopathy. In all three cases a relationship was demonstrated between the dentate nucleus and dento-olivary pathway - a relation of topical order in some instances - and the hypertrophic lesion of the inferior olive. Examination in the electron microscope revealed in the hypertrophic olivary cell not only dystrophic lesions but also progressive intracellular phenomena, such as maintenance of the Nissl bodies, multiplication of the mitochondria and especially marked proliferation of the neuro-filaments. This shows that, at least in some of the evolutive phases, hypertrophy of the inferior olives is based upon a real hypertrophy of the olivary cell. In addition, lesions of the glial system of the hypertrophic inferior olives were noted with the presence of osmiophil degeneration whose morphology clearly differed from that of Rosenthal's fibres.", "PMID": 968313}
{"id": "pubmed23n0032_7262", "title": "[Meningeal arterio-venous fistulas with cortical venous drainage].", "content": "Pure dural arteriovenous fistulas are still not properly understood, and pose problems wich are far from being resolved. Among these, dural fistulas with cortical venous drainage represent a very special entity since they alone are expressed clinically by a neurological brain syndrome. Diagnosis is difficult and mistakes can be made as it is only by means of hyperselective arteriography of the branches of the external carotid that an accurate diagnosis can be made. However, their diagnosis assumes considerable importance since surgical or radiological treatment of hese fistulas brings about complete disappearance of clinical symptoms.", "contents": "[Meningeal arterio-venous fistulas with cortical venous drainage]. Pure dural arteriovenous fistulas are still not properly understood, and pose problems wich are far from being resolved. Among these, dural fistulas with cortical venous drainage represent a very special entity since they alone are expressed clinically by a neurological brain syndrome. Diagnosis is difficult and mistakes can be made as it is only by means of hyperselective arteriography of the branches of the external carotid that an accurate diagnosis can be made. However, their diagnosis assumes considerable importance since surgical or radiological treatment of hese fistulas brings about complete disappearance of clinical symptoms.", "PMID": 968314}
{"id": "pubmed23n0032_7263", "title": "[Catecholaminergic axons in the human cerebral cortex. Observation by histofluorescence of cerebral biopsies in 2 cases of Alzheimer's disease].", "content": "Catecholamine axons have been visualized in human cerebral cortex obtained during routine neurosurgical operations. The fluorescence histochemical method of Lindvall et al. was used, slightly modified (calcium-deprived buffer, glyoxylic acid fixation followed by formaldehyde vapours exposition). The frontal cortex was more richely provided with catecholamine terminals than the parietal cortex. Two general types of axon morphology are evident. The most frequent is thin and sinous, sometimes forming clews, or loose basket-like arrangement around presumed nerve cells. The other one is moniliform and demonstrates spherical evenly-spaced varicosities. They look like, respectively, the well characterized dopaminergic and noradrenergic axons of the rat cerebral cortex. In two cases of Alzheimer's disease, noradrenergic-like fibers were missing and voluminous green-fluorescent varicosities, sometimes in obvious connection with typical axons, were observed in the proximity of senile plaques.", "contents": "[Catecholaminergic axons in the human cerebral cortex. Observation by histofluorescence of cerebral biopsies in 2 cases of Alzheimer's disease]. Catecholamine axons have been visualized in human cerebral cortex obtained during routine neurosurgical operations. The fluorescence histochemical method of Lindvall et al. was used, slightly modified (calcium-deprived buffer, glyoxylic acid fixation followed by formaldehyde vapours exposition). The frontal cortex was more richely provided with catecholamine terminals than the parietal cortex. Two general types of axon morphology are evident. The most frequent is thin and sinous, sometimes forming clews, or loose basket-like arrangement around presumed nerve cells. The other one is moniliform and demonstrates spherical evenly-spaced varicosities. They look like, respectively, the well characterized dopaminergic and noradrenergic axons of the rat cerebral cortex. In two cases of Alzheimer's disease, noradrenergic-like fibers were missing and voluminous green-fluorescent varicosities, sometimes in obvious connection with typical axons, were observed in the proximity of senile plaques.", "PMID": 968315}
{"id": "pubmed23n0032_7264", "title": "[Variations in local cerebral blood volume depending on the mean arterial blood pressure in man].", "content": "A radio isotopic technique is described to measure and monitor the cerebral blood volume during a controlled arterial hypotension induced by sodium nitroprusside in anesthetised patients (neurolepanalgesia) submited to surgery for a ruptured saccular aneurysm. The study was made in 10 cases from 90 mm Hg to 30 mm Hg mean blood pressure monitored from a transducer connected to an intra humeral microcatheter. The figures obtained show an increase of the cerebral blood volume from 90 mm Hg to 50 mm Hg in every patient and a linear correlation when the pressure is lowered under 50 mm Hg level; therefore under a mean arterial pressure of 50 mm Hg the autoregulation arterial mechanism is out of work.", "contents": "[Variations in local cerebral blood volume depending on the mean arterial blood pressure in man]. A radio isotopic technique is described to measure and monitor the cerebral blood volume during a controlled arterial hypotension induced by sodium nitroprusside in anesthetised patients (neurolepanalgesia) submited to surgery for a ruptured saccular aneurysm. The study was made in 10 cases from 90 mm Hg to 30 mm Hg mean blood pressure monitored from a transducer connected to an intra humeral microcatheter. The figures obtained show an increase of the cerebral blood volume from 90 mm Hg to 50 mm Hg in every patient and a linear correlation when the pressure is lowered under 50 mm Hg level; therefore under a mean arterial pressure of 50 mm Hg the autoregulation arterial mechanism is out of work.", "PMID": 968316}
{"id": "pubmed23n0032_7265", "title": "[Study on the intraspinal cord regeneration of sensory fibers sectioned in the posterior root of the cat].", "content": "In order to assess the potential ability for posterior column fibers to repair, cat sensory root fibers where their repair is known to be possible. Progress of the outgrowth of fibers into the spinal cord (posterior columns) was tested by observation of the animals, histological investigation of the roots, examination of the posterior columns on cross-section at various levels, and by electrological investigation of conduction from root to spinal cord. The results obtained indicate that repair of root fibers may continue into the spinal cord, both segmentally and all along the posterior columns. These results are discussed and compared with failure of repair of posterior column fibers after spinal cord section.", "contents": "[Study on the intraspinal cord regeneration of sensory fibers sectioned in the posterior root of the cat]. In order to assess the potential ability for posterior column fibers to repair, cat sensory root fibers where their repair is known to be possible. Progress of the outgrowth of fibers into the spinal cord (posterior columns) was tested by observation of the animals, histological investigation of the roots, examination of the posterior columns on cross-section at various levels, and by electrological investigation of conduction from root to spinal cord. The results obtained indicate that repair of root fibers may continue into the spinal cord, both segmentally and all along the posterior columns. These results are discussed and compared with failure of repair of posterior column fibers after spinal cord section.", "PMID": 968318}
{"id": "pubmed23n0032_7266", "title": "[Nosographic approach of heriditary myopathies. Attempt at classification and diagnosis by means of erythrocyte cholinesterase and serum pseudocholinesterase analysis].", "content": "An original biological study involving assessment of red cell cholinesterase and serum pseudo-cholinesterase activity has given the authors a new approach to the classification of progressive muscular dystrophy: as a result, it has become possible to isolate Duchenne dystrophy and the carries of this disease and to distinguish them from cases of Becker's disease. Also, Leyden-M\u00f6bius dystrophy appears to deserve the name as it differs from limb-girdle dystrophy. Finally, there is a case to be made for classifying separately, because of its special biological characteristics, Steinert's myotonic dystrophy.", "contents": "[Nosographic approach of heriditary myopathies. Attempt at classification and diagnosis by means of erythrocyte cholinesterase and serum pseudocholinesterase analysis]. An original biological study involving assessment of red cell cholinesterase and serum pseudo-cholinesterase activity has given the authors a new approach to the classification of progressive muscular dystrophy: as a result, it has become possible to isolate Duchenne dystrophy and the carries of this disease and to distinguish them from cases of Becker's disease. Also, Leyden-M\u00f6bius dystrophy appears to deserve the name as it differs from limb-girdle dystrophy. Finally, there is a case to be made for classifying separately, because of its special biological characteristics, Steinert's myotonic dystrophy.", "PMID": 968319}
{"id": "pubmed23n0032_7267", "title": "[Childhood aphasia with epilepsy. Remission with antiepileptic treatment].", "content": "The authors describe an example of an aphasic syndrome peculiar to children. This syndrome, which has previously been described by several authors, is characterized by an unusual form of aphasia, by its association with epileptic attacks, and by its onset in the absence of any apparent aetiology. In such cases, the effectiveness of anti-epileptic treatment on the aphasia is variable, often quite poor; on the other hand, in the case described in this report, administration of anti-epileptic treatment was followed by spectacular regression of the aphasia.", "contents": "[Childhood aphasia with epilepsy. Remission with antiepileptic treatment]. The authors describe an example of an aphasic syndrome peculiar to children. This syndrome, which has previously been described by several authors, is characterized by an unusual form of aphasia, by its association with epileptic attacks, and by its onset in the absence of any apparent aetiology. In such cases, the effectiveness of anti-epileptic treatment on the aphasia is variable, often quite poor; on the other hand, in the case described in this report, administration of anti-epileptic treatment was followed by spectacular regression of the aphasia.", "PMID": 968320}
{"id": "pubmed23n0032_7268", "title": "[Hodgkin's granuloma of the cerebral hemisphere. Apropos of one case].", "content": "With reference to a case of Hodgkin's granuloma of the frontal lobe, a review of the literature has enabled 8 similar cases of intra-cerebral localisation of this disease to be reclassified. Their mechanism is discussed on the basis of neuropathological data and in particular their development in situ. Excision of the lesion appears to be the treatment of choice.", "contents": "[Hodgkin's granuloma of the cerebral hemisphere. Apropos of one case]. With reference to a case of Hodgkin's granuloma of the frontal lobe, a review of the literature has enabled 8 similar cases of intra-cerebral localisation of this disease to be reclassified. Their mechanism is discussed on the basis of neuropathological data and in particular their development in situ. Excision of the lesion appears to be the treatment of choice.", "PMID": 968321}
{"id": "pubmed23n0032_7269", "title": "Synovial fluid in the calf.", "content": "While awareness of synovial rupture of the knee joint confirmed by early arthrography increases, concomitant deep-vein occlusion may be being overlooked. Two cases of this are described, illustrating different mechanisms of venous occlusion by synovial rupture. Early thrombosis occurred in one patient from direct pressure as a result of haematoma following anticoagulant therapy and in another due to presumed external irritation of the vein. Although isolated bursae do not apparently rupture, tendon sheaths occasionally do. An example of lateral popliteal mononeuritis as a result of rupture of a tense peroneal tendon sheath is also given. Synovial rupture is primarily a clinical diagnosis but in selected cases, deep calf venography may be useful rather than arthrography. Almost a century ago, William Morrant Baker published his work on synovial protrusions showing his awareness of the consequences of free fluid in the calf. Some comments are made on his life and work.", "contents": "Synovial fluid in the calf. While awareness of synovial rupture of the knee joint confirmed by early arthrography increases, concomitant deep-vein occlusion may be being overlooked. Two cases of this are described, illustrating different mechanisms of venous occlusion by synovial rupture. Early thrombosis occurred in one patient from direct pressure as a result of haematoma following anticoagulant therapy and in another due to presumed external irritation of the vein. Although isolated bursae do not apparently rupture, tendon sheaths occasionally do. An example of lateral popliteal mononeuritis as a result of rupture of a tense peroneal tendon sheath is also given. Synovial rupture is primarily a clinical diagnosis but in selected cases, deep calf venography may be useful rather than arthrography. Almost a century ago, William Morrant Baker published his work on synovial protrusions showing his awareness of the consequences of free fluid in the calf. Some comments are made on his life and work.", "PMID": 968335}
{"id": "pubmed23n0032_7270", "title": "Spinal changes in idiopathic chondrocalcinosis articularis.", "content": "The lumbar-spine radiographs of 67 patients with idiopathic chondrocalcinosis articularis were reviewed for disc calcification and other changes. Calcification was present in 21 (31%) of the patients, seen most frequently at the L 2-3 disc space. The 21 patients as a group were significantly older than the 46 patients without disc calcification, and also had a much higher incidence of chondrocalcinosis in peripheral joints. There was no association with back pain or spinal stiffness. The 21 patients with disc calcification included six patients with a destructive peripheral arthropathy, and three of them had destructive changes affecting the lumbar spine. Three patients with a destructive peripheral arthropathy were also included in the group without disc calcification, and one of these had a destructive arthritis of the lumbar spine. For comparison, there was a 55% incidence of spinal chondrocalcinosis in nine patients with primary hyperparathyroidism and peripheral joint chondrocalcinosis, and a 6% incidence in 100 anteroposterior lumbar spine radiograph 'controls', taken before intravenous urography, although in this latter group the changes were minimal and confined to the margin of a single disc in each case.", "contents": "Spinal changes in idiopathic chondrocalcinosis articularis. The lumbar-spine radiographs of 67 patients with idiopathic chondrocalcinosis articularis were reviewed for disc calcification and other changes. Calcification was present in 21 (31%) of the patients, seen most frequently at the L 2-3 disc space. The 21 patients as a group were significantly older than the 46 patients without disc calcification, and also had a much higher incidence of chondrocalcinosis in peripheral joints. There was no association with back pain or spinal stiffness. The 21 patients with disc calcification included six patients with a destructive peripheral arthropathy, and three of them had destructive changes affecting the lumbar spine. Three patients with a destructive peripheral arthropathy were also included in the group without disc calcification, and one of these had a destructive arthritis of the lumbar spine. For comparison, there was a 55% incidence of spinal chondrocalcinosis in nine patients with primary hyperparathyroidism and peripheral joint chondrocalcinosis, and a 6% incidence in 100 anteroposterior lumbar spine radiograph 'controls', taken before intravenous urography, although in this latter group the changes were minimal and confined to the margin of a single disc in each case.", "PMID": 968336}
{"id": "pubmed23n0032_7271", "title": "The great toe as a clinical problem in rheumatoid arthritis.", "content": "Two hundred consecutive in-patients with rheumatoid arthritis were examined for pain or deformity of the feet, and of the great toe in particular. Some abnormality occured in 196 feet and the deformities observed are presented. The symptoms that arise from these deformities are mainly derived from ill-fitting shoes, and the need for suitable foot-wear is emphasized. Two hitherto un-named entities are described namely Hallux tortus and chisel toe, since they give rise to their own shoe-fitting problems.", "contents": "The great toe as a clinical problem in rheumatoid arthritis. Two hundred consecutive in-patients with rheumatoid arthritis were examined for pain or deformity of the feet, and of the great toe in particular. Some abnormality occured in 196 feet and the deformities observed are presented. The symptoms that arise from these deformities are mainly derived from ill-fitting shoes, and the need for suitable foot-wear is emphasized. Two hitherto un-named entities are described namely Hallux tortus and chisel toe, since they give rise to their own shoe-fitting problems.", "PMID": 968337}
{"id": "pubmed23n0032_7272", "title": "The effects of antacids on enteric-coated salicylate preparations.", "content": "A volunteer study was undertaken in which the effect of the co-administration of therapeutic doses of aluminium hydroxide and magnesium trisilicate on the excretion of aspirin derived from enteric-coated preparations was studied. A significant alteration in the pattern of salicylate excretion was seen, but the mechanism of the interaction cannot be deduced from the present study. It was concluded that the interaction was of potential therapeutic importance, and further studies based on this pilot investigation have been initiated.", "contents": "The effects of antacids on enteric-coated salicylate preparations. A volunteer study was undertaken in which the effect of the co-administration of therapeutic doses of aluminium hydroxide and magnesium trisilicate on the excretion of aspirin derived from enteric-coated preparations was studied. A significant alteration in the pattern of salicylate excretion was seen, but the mechanism of the interaction cannot be deduced from the present study. It was concluded that the interaction was of potential therapeutic importance, and further studies based on this pilot investigation have been initiated.", "PMID": 968338}
{"id": "pubmed23n0032_7273", "title": "Muscle strength before and after menisectomy: a comparison of methods of post-operative management.", "content": "In patients who have undergone menisectomy, isometric strength of the extensor and flexor muscles of the knee improves more rapidly when flexion exercises are encouraged. Immobilizing the knee joint in a back-slab and restricting the patient to static bracing of the quadriceps delays the recovery of strength, particularly in the more flexed positions of the knee joint.", "contents": "Muscle strength before and after menisectomy: a comparison of methods of post-operative management. In patients who have undergone menisectomy, isometric strength of the extensor and flexor muscles of the knee improves more rapidly when flexion exercises are encouraged. Immobilizing the knee joint in a back-slab and restricting the patient to static bracing of the quadriceps delays the recovery of strength, particularly in the more flexed positions of the knee joint.", "PMID": 968339}
{"id": "pubmed23n0032_7274", "title": "The effect of rheumatoid arthritis on the social situation of housewives.", "content": "Thirty-seven housewives treated in hospital for rheumatoid arthritis were investigated with particular reference to their husbands' reactions. Although most patients had 'accepted' the disease, many were distressed by both the physical aspects of it (tiredness, pain and limitation of activities) and by the psychological aspects (guilt, fear of being a burden, and not understanding the disease). In 70% it was felt that their husbands understood the disease, but half the patients had difficulties in coping with the anxiety of their husbands. Those whose arthritis had developed before marriage fared better in terms of fear of losing their independence, understanding the disease, guilt feelings, adjustment to the disease, their husbands' understanding and friction in the home. Patients with milder disease were more anxious, but experienced the greater understanding of their husbands. Help was required in enabling the patients to cope with their fears, to manage practically, and to deal with the anxiety of their husbands. Help in coping with the fears and in allaying the anxiety of their husbands was most required in those with mild disease and those in whom it had progressed to become socially disabling, but without confining the patients to their homes.", "contents": "The effect of rheumatoid arthritis on the social situation of housewives. Thirty-seven housewives treated in hospital for rheumatoid arthritis were investigated with particular reference to their husbands' reactions. Although most patients had 'accepted' the disease, many were distressed by both the physical aspects of it (tiredness, pain and limitation of activities) and by the psychological aspects (guilt, fear of being a burden, and not understanding the disease). In 70% it was felt that their husbands understood the disease, but half the patients had difficulties in coping with the anxiety of their husbands. Those whose arthritis had developed before marriage fared better in terms of fear of losing their independence, understanding the disease, guilt feelings, adjustment to the disease, their husbands' understanding and friction in the home. Patients with milder disease were more anxious, but experienced the greater understanding of their husbands. Help was required in enabling the patients to cope with their fears, to manage practically, and to deal with the anxiety of their husbands. Help in coping with the fears and in allaying the anxiety of their husbands was most required in those with mild disease and those in whom it had progressed to become socially disabling, but without confining the patients to their homes.", "PMID": 968340}
{"id": "pubmed23n0032_7275", "title": "Application of visual-analogue scales to the measurement of functional capacity.", "content": "Visual-analogue scales can be used to measure the subjective aspect of functional ability but, in a group of patients with severe rheumatoid arthritis, the severity of impairment of two predetermined functional tests was low in comparison with the severity of pain. Measurements of the ability to carry out these two functions, using visual-analogue and descriptive scales, and also using the time taken to perform the tasks, were not useful measures of the effectiveness of treatment of rheumatoid arthritis. Poor correlations were obtained between different measures of the same function and between different functions measured in the same way.", "contents": "Application of visual-analogue scales to the measurement of functional capacity. Visual-analogue scales can be used to measure the subjective aspect of functional ability but, in a group of patients with severe rheumatoid arthritis, the severity of impairment of two predetermined functional tests was low in comparison with the severity of pain. Measurements of the ability to carry out these two functions, using visual-analogue and descriptive scales, and also using the time taken to perform the tasks, were not useful measures of the effectiveness of treatment of rheumatoid arthritis. Poor correlations were obtained between different measures of the same function and between different functions measured in the same way.", "PMID": 968347}
{"id": "pubmed23n0032_7276", "title": "Cyclophosphamide, gold and penicillamine--disease-modifying drugs in rheumatoid arthritis--tailored dosage and ultimate success.", "content": "Gold appears to be the disease-modifying drug of first choice for two reasons: response and absence of long-term toxicity. Although cyclophosphamide produces results as good or better than gold, the possible long-term toxicity outweighs the clinical benefit except in the unusual case. Penicillamine appears to be an acceptable drug of second choice.", "contents": "Cyclophosphamide, gold and penicillamine--disease-modifying drugs in rheumatoid arthritis--tailored dosage and ultimate success. Gold appears to be the disease-modifying drug of first choice for two reasons: response and absence of long-term toxicity. Although cyclophosphamide produces results as good or better than gold, the possible long-term toxicity outweighs the clinical benefit except in the unusual case. Penicillamine appears to be an acceptable drug of second choice.", "PMID": 968355}
{"id": "pubmed23n0032_7277", "title": "[Axial computer tomography of the brain].", "content": "Computertized tomography of the brain, developed by Hounsfield - has decisively altered neuroradiologic procedures. Without the need of contrast media, cerebral structures (ventricles cisternes, sulci and physiological calcifications) and pathologic lesions (infarctions, hemorrhage, edema, tumors and malformations) may be visualized. Tumors can be diagnosed in 95 to 99% by this method. Tumors of the midline structures and of the posterior fossa are more easily detected than by conventional methods. In head injuries epidural and subdural hematoma, contusional edema and hemorrhage may be differentiated. Hydrocephalus externus and internus can be shown by computertized tomography. Probably computertized tomography will become the most frequently applied neuroradiological method in the future.", "contents": "[Axial computer tomography of the brain]. Computertized tomography of the brain, developed by Hounsfield - has decisively altered neuroradiologic procedures. Without the need of contrast media, cerebral structures (ventricles cisternes, sulci and physiological calcifications) and pathologic lesions (infarctions, hemorrhage, edema, tumors and malformations) may be visualized. Tumors can be diagnosed in 95 to 99% by this method. Tumors of the midline structures and of the posterior fossa are more easily detected than by conventional methods. In head injuries epidural and subdural hematoma, contusional edema and hemorrhage may be differentiated. Hydrocephalus externus and internus can be shown by computertized tomography. Probably computertized tomography will become the most frequently applied neuroradiological method in the future.", "PMID": 968371}
{"id": "pubmed23n0032_7278", "title": "[Surgery of gastric and duodenal ulcer].", "content": "The variety of surgical techniques to deal with gastric and duodenal ulcers has been increased by non-resecting methods (truncular, selective, selective proximal vagotomy with or without pyloroplasty). Selective proximal vagotomy has proved its worth in uncomplicated chronic duodenal ulcer. Additional drainage is needed when there is pyloric stenosis. In 309 patients of the surgical department of Cologne-Lindenthal in the years 1970 to June 1975 selective proximal vagotomy for chronic duodenal ulcer had an operative mortality of 0.7%. 1-5 years later 88% of patients had no recurrence. The main cause of recurrence is incomplete vagotomy which could be avoided by the routine intra-operative use of the electrostimulator of Burge. In chronic gastric ulcer removal of the ulcer is needed because of the danger of malignant degreneration. Two third resection is the standard technique. In patients at risk vagotomy, pyloroplasty and excision of the ulcer are indicated.", "contents": "[Surgery of gastric and duodenal ulcer]. The variety of surgical techniques to deal with gastric and duodenal ulcers has been increased by non-resecting methods (truncular, selective, selective proximal vagotomy with or without pyloroplasty). Selective proximal vagotomy has proved its worth in uncomplicated chronic duodenal ulcer. Additional drainage is needed when there is pyloric stenosis. In 309 patients of the surgical department of Cologne-Lindenthal in the years 1970 to June 1975 selective proximal vagotomy for chronic duodenal ulcer had an operative mortality of 0.7%. 1-5 years later 88% of patients had no recurrence. The main cause of recurrence is incomplete vagotomy which could be avoided by the routine intra-operative use of the electrostimulator of Burge. In chronic gastric ulcer removal of the ulcer is needed because of the danger of malignant degreneration. Two third resection is the standard technique. In patients at risk vagotomy, pyloroplasty and excision of the ulcer are indicated.", "PMID": 968373}
{"id": "pubmed23n0032_7279", "title": "[Radiologic diagnosis of retrograde jejunogastric invagination following gastrectomy].", "content": "Retrograde jejunogastric invagination is a rare late complication of operations on the stomach. Clinically signs and symptoms (colic-like upper abdominal pain, cyclindrical resistance and hematemesis) resemble that of high ileus. This complication, when wrongly interpreted or unknown, has a high mortality. One case following delivery shows the radiologic signs.", "contents": "[Radiologic diagnosis of retrograde jejunogastric invagination following gastrectomy]. Retrograde jejunogastric invagination is a rare late complication of operations on the stomach. Clinically signs and symptoms (colic-like upper abdominal pain, cyclindrical resistance and hematemesis) resemble that of high ileus. This complication, when wrongly interpreted or unknown, has a high mortality. One case following delivery shows the radiologic signs.", "PMID": 968374}
{"id": "pubmed23n0032_7280", "title": "[Localisation of mammographic findings, suspect of malignancy but not palpable, and their histologic patterns].", "content": "A technique of marking non-palpable changes in mammograms for the surgeon is described. The suspected area is injected with contrast medium and patent blue. The radiologist can assess the exact site and the surgeon can recognize it at operation. Radiographs of the specimen removed can make sure whether the suspect area was removed. There were 13 preclinical cancers, 2 cancers in site, 1 sclerosing adenosis, 1 proliferating mastopathy, and 66 fibrous or fibrocytic mastopathies. Among contrast media Urografin 76% appears the best because of its sufficient contrast density and complete reabsorbtion. So far there have been no serious complications", "contents": "[Localisation of mammographic findings, suspect of malignancy but not palpable, and their histologic patterns]. A technique of marking non-palpable changes in mammograms for the surgeon is described. The suspected area is injected with contrast medium and patent blue. The radiologist can assess the exact site and the surgeon can recognize it at operation. Radiographs of the specimen removed can make sure whether the suspect area was removed. There were 13 preclinical cancers, 2 cancers in site, 1 sclerosing adenosis, 1 proliferating mastopathy, and 66 fibrous or fibrocytic mastopathies. Among contrast media Urografin 76% appears the best because of its sufficient contrast density and complete reabsorbtion. So far there have been no serious complications", "PMID": 968375}
{"id": "pubmed23n0032_7281", "title": "[Orbital phlebography].", "content": "For the diagnosis of intra- and retro-orbital processes, orbital phlebography occupies a key position whereas carotid arteriography visualizing the ophthalmic artery has only a limited diagnostic value for the assessment of absolute intra-orbital processes. Use of subtraction method and magnification technique brought essential improvement to the vessel representation of the orbit. Combination of these techniques in connection with computerized axial tomography (EMI-Scanner), as a rule, allows localization and identification of intraorbital space-occupying processes without difficulty.", "contents": "[Orbital phlebography]. For the diagnosis of intra- and retro-orbital processes, orbital phlebography occupies a key position whereas carotid arteriography visualizing the ophthalmic artery has only a limited diagnostic value for the assessment of absolute intra-orbital processes. Use of subtraction method and magnification technique brought essential improvement to the vessel representation of the orbit. Combination of these techniques in connection with computerized axial tomography (EMI-Scanner), as a rule, allows localization and identification of intraorbital space-occupying processes without difficulty.", "PMID": 968376}
{"id": "pubmed23n0032_7282", "title": "[Important radiologic findings following blunt thoracic trauma (author's transl)].", "content": "In patients suffering from traffic accidents traumatic lesions of the chest are of special importance since in  50% of deaths the thoracic trauma is responsible for the final outcome. The fatal course is, however, not always due to severe and irreparable dammage to thoracic organs, but very often due to under-estimation of initial symptoms and thus inadequate treatment. For the proper diagnosis of traumatic lesions of the thorax the plain film of the chest is, therefore, fundamentally important. In this paper, which is mainly dealing with lesions of the airways and lungs, the significance of radiological symptoms is stressed and a correlation with pathophysiologic as well as histologic data is attempted.", "contents": "[Important radiologic findings following blunt thoracic trauma (author's transl)]. In patients suffering from traffic accidents traumatic lesions of the chest are of special importance since in  50% of deaths the thoracic trauma is responsible for the final outcome. The fatal course is, however, not always due to severe and irreparable dammage to thoracic organs, but very often due to under-estimation of initial symptoms and thus inadequate treatment. For the proper diagnosis of traumatic lesions of the thorax the plain film of the chest is, therefore, fundamentally important. In this paper, which is mainly dealing with lesions of the airways and lungs, the significance of radiological symptoms is stressed and a correlation with pathophysiologic as well as histologic data is attempted.", "PMID": 968377}
{"id": "pubmed23n0032_7283", "title": "[Diagnostic radiology in the field of traumatology (author's transl)].", "content": "Diagnostic radiology is important for casualty surgery. Evaluation of the valuable radiological indications presupposes knowledge of and experience in tactical and technical procedures. Knowledge of the biochemical processes, in the area of a fracture is also essential. The most important criteria were briefly presented.", "contents": "[Diagnostic radiology in the field of traumatology (author's transl)]. Diagnostic radiology is important for casualty surgery. Evaluation of the valuable radiological indications presupposes knowledge of and experience in tactical and technical procedures. Knowledge of the biochemical processes, in the area of a fracture is also essential. The most important criteria were briefly presented.", "PMID": 968378}
{"id": "pubmed23n0032_7284", "title": "[The value of ossovenography in advanced female genital carcinoma. A contribution to diagnosis of combined obstructive edema (author's transl)].", "content": "The pertrochanteric ossovenography brought important results on the etiology of combined obstructive edema of the lower limb after genital carcinoma. Its advantage in comparison with other methods of phlebography is the lack of embolic danger for the patient, its suitability even in thrombosis of the V. femoralis and the optimal contrast staining of venous system and anastomoses. The combined obstructive edema is characterized by a block of pelvic veins and a complete or incomplete block of lymph vessels. The combined obstructive edema depends only on the venous block as is demonstrated. A report on therapy and prognosis of 30 cases is given.", "contents": "[The value of ossovenography in advanced female genital carcinoma. A contribution to diagnosis of combined obstructive edema (author's transl)]. The pertrochanteric ossovenography brought important results on the etiology of combined obstructive edema of the lower limb after genital carcinoma. Its advantage in comparison with other methods of phlebography is the lack of embolic danger for the patient, its suitability even in thrombosis of the V. femoralis and the optimal contrast staining of venous system and anastomoses. The combined obstructive edema is characterized by a block of pelvic veins and a complete or incomplete block of lymph vessels. The combined obstructive edema depends only on the venous block as is demonstrated. A report on therapy and prognosis of 30 cases is given.", "PMID": 968379}
{"id": "pubmed23n0032_7285", "title": "[Bilateral breast cancer (author's transl)].", "content": "The frequency of bilateral primary (simultaneous and nonsimultaneous) breast cancer in 1420 patients was reported. The number of cases of bilateral breast cancer not caused by metastases amounted to 1,75%. The problems and significance of follow-up checks was discussed.", "contents": "[Bilateral breast cancer (author's transl)]. The frequency of bilateral primary (simultaneous and nonsimultaneous) breast cancer in 1420 patients was reported. The number of cases of bilateral breast cancer not caused by metastases amounted to 1,75%. The problems and significance of follow-up checks was discussed.", "PMID": 968380}
{"id": "pubmed23n0032_7286", "title": "Autophagia in myeloid precursors: an explanation for neutropenia in Chediak-Higashi syndrome?", "content": "Neutropenia is an almost constant feature of Chediak-Higashi syndrome (CHS). There is evidence for a central mechanism of neutropenia. Ultrastructural studies of the bone marrow from a child with CHS showed marked autophagic phenomena within myeloid precursor cells and mature neutrophils. Autophagic vacuoles were randomly distributed in the cytoplasm of the cells from the granulocytic series and some of them contained giant granules which thus appeared particularly resistant to the autophagic process. The vital cellular damage through endophagocytosis suggests the possibility of intramedullary destruction as an explanation for neutropenia.", "contents": "Autophagia in myeloid precursors: an explanation for neutropenia in Chediak-Higashi syndrome? Neutropenia is an almost constant feature of Chediak-Higashi syndrome (CHS). There is evidence for a central mechanism of neutropenia. Ultrastructural studies of the bone marrow from a child with CHS showed marked autophagic phenomena within myeloid precursor cells and mature neutrophils. Autophagic vacuoles were randomly distributed in the cytoplasm of the cells from the granulocytic series and some of them contained giant granules which thus appeared particularly resistant to the autophagic process. The vital cellular damage through endophagocytosis suggests the possibility of intramedullary destruction as an explanation for neutropenia.", "PMID": 968442}
{"id": "pubmed23n0032_7287", "title": "Plasma iron and erythrocytic glutathione peroxidase activity. A possible mechanism for oxidative haemolysis in iron deficiency anemia.", "content": "The red cell glutathione-peroxidase (GSH-Px) activity of 9 normal subjects is compared with that of 15 cases of iron deficiency anaemia and with 13 cases of heterozygous beta-thalassemia with the same degree of anaemia and hypochromia. 2 cases of sideroblastic anaemia with high serum iron levels were also examined. Enzymatic activity was found to be significantly decreased in iron deficiency anaemia (about 55% of normal range), while it was not affected in heterozygous beta thalassaemia and it was increased in the 2 cases of sideroblastic anaemia. Moreover, GSH-Px activity exhibited a significant correlation with serum iron levels in all the patients studied. The observed modifications in GSH-Px activity are not correlated with erythrocyte ageing because reticulocyte-poor fractions exhibited GSH-Px activity which was not significantly reduced in respect of the reticulocyte-rich ones. These data seem to suggest that iron has a crucial connection with erythrocyte GSH-Px and that the enzyme deficiency may be of some importance in explaining the decreased red cell survival observed in severe iron-deficiency anaemias.", "contents": "Plasma iron and erythrocytic glutathione peroxidase activity. A possible mechanism for oxidative haemolysis in iron deficiency anemia. The red cell glutathione-peroxidase (GSH-Px) activity of 9 normal subjects is compared with that of 15 cases of iron deficiency anaemia and with 13 cases of heterozygous beta-thalassemia with the same degree of anaemia and hypochromia. 2 cases of sideroblastic anaemia with high serum iron levels were also examined. Enzymatic activity was found to be significantly decreased in iron deficiency anaemia (about 55% of normal range), while it was not affected in heterozygous beta thalassaemia and it was increased in the 2 cases of sideroblastic anaemia. Moreover, GSH-Px activity exhibited a significant correlation with serum iron levels in all the patients studied. The observed modifications in GSH-Px activity are not correlated with erythrocyte ageing because reticulocyte-poor fractions exhibited GSH-Px activity which was not significantly reduced in respect of the reticulocyte-rich ones. These data seem to suggest that iron has a crucial connection with erythrocyte GSH-Px and that the enzyme deficiency may be of some importance in explaining the decreased red cell survival observed in severe iron-deficiency anaemias.", "PMID": 968443}
{"id": "pubmed23n0032_7288", "title": "Effect of hyperlipidaemic diet and clofibrate treatment on rat erythrocyte lipids.", "content": "Lipids of rat plasma and erythrocytes were analysed during hyperlipidaemic diet and clofibrate treatment. The diet increased the plasma cholesterol and phospholipid concentrations after 3 weeks' lipid feeding. Clofibrate in a dose of 200 mg/kg body weight did not inhibit the increase in plasma cholesterol and phospholipid contents, but significantly decreased the concentration of plasma triglycerides. The lipid diet slightly increased the erythrocyte cholesterol and phospholipid concentrations without affecting the red cell morphology or reticulocytosis. After 1 week's treatment, clofibrate in a dose of 200 mg/kg body weight caused a 2-fold increase in the cholesterol content and a rise in the cholesterol-phospholipid ratio of red blood cells of rats fed the hyperlipidaemic diet. These results suggest that the erythrocyte cell membrane can increase its cholesterol content and possibly play a role in cholesterol transport.", "contents": "Effect of hyperlipidaemic diet and clofibrate treatment on rat erythrocyte lipids. Lipids of rat plasma and erythrocytes were analysed during hyperlipidaemic diet and clofibrate treatment. The diet increased the plasma cholesterol and phospholipid concentrations after 3 weeks' lipid feeding. Clofibrate in a dose of 200 mg/kg body weight did not inhibit the increase in plasma cholesterol and phospholipid contents, but significantly decreased the concentration of plasma triglycerides. The lipid diet slightly increased the erythrocyte cholesterol and phospholipid concentrations without affecting the red cell morphology or reticulocytosis. After 1 week's treatment, clofibrate in a dose of 200 mg/kg body weight caused a 2-fold increase in the cholesterol content and a rise in the cholesterol-phospholipid ratio of red blood cells of rats fed the hyperlipidaemic diet. These results suggest that the erythrocyte cell membrane can increase its cholesterol content and possibly play a role in cholesterol transport.", "PMID": 968444}
{"id": "pubmed23n0032_7289", "title": "Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat.", "content": "The proliferating granulocyte precursor pool of rat bone marrow was labelled during DNA synthesis by continuous infusion and by single injection of 3H-thymidine (3H-TdR), as well as by single injection of 125I-iododeoxyuridine (125I-UdR). The appearance of neutrophilic granulocytes in the blood stream after these various labelling procedures was studied by autoradiography. Labelling patterns of blood neutrophils were identical during continuous infusion and after single injection of 3H-TdR, and 100% labelling of the blood compartment was achieved. This result indicated reutilization of DNA catabolites to occur in granulocytopoiesis leading to continuous availability of 3H-labelled DNA precursors even after a single injection of 3H-TdR. Attempts to suppress reutilization of label by infusion of cold thymidine 1 h after injection of 3H-TdR were unsuccessful. However, a change in the labelling pattern of blood neutrophils was seen after single injection of 125I-UdR, a DNA precursor poorly reutilized in comparison to 3H-TdR. This result provided further evidence for reutilization of DNA catabolites by the cell system investigated. A comprehensive discussion of the results indicates that thymidinemonophosphate is the biochemical level of reutilization in granulocytopoiesis.", "contents": "Autoradiographic evidence for reutilization of DNA catabolites by granulocytopoiesis in the rat. The proliferating granulocyte precursor pool of rat bone marrow was labelled during DNA synthesis by continuous infusion and by single injection of 3H-thymidine (3H-TdR), as well as by single injection of 125I-iododeoxyuridine (125I-UdR). The appearance of neutrophilic granulocytes in the blood stream after these various labelling procedures was studied by autoradiography. Labelling patterns of blood neutrophils were identical during continuous infusion and after single injection of 3H-TdR, and 100% labelling of the blood compartment was achieved. This result indicated reutilization of DNA catabolites to occur in granulocytopoiesis leading to continuous availability of 3H-labelled DNA precursors even after a single injection of 3H-TdR. Attempts to suppress reutilization of label by infusion of cold thymidine 1 h after injection of 3H-TdR were unsuccessful. However, a change in the labelling pattern of blood neutrophils was seen after single injection of 125I-UdR, a DNA precursor poorly reutilized in comparison to 3H-TdR. This result provided further evidence for reutilization of DNA catabolites by the cell system investigated. A comprehensive discussion of the results indicates that thymidinemonophosphate is the biochemical level of reutilization in granulocytopoiesis.", "PMID": 968445}
{"id": "pubmed23n0032_7290", "title": "Testicular function after radiotherapy to inverted 'Y' field for malignant lymphoma.", "content": "Testicular function was estimated by sperm counts, hormone assays and recording of reported conceptions in 9 patients irradiated for malignant lymphoma. The treatment had been an inverted 'Y' field including the inguinal regions with, in addition, a mantle field in 8 patients. Azoospermia or severe oligozoospermia was found in all but 1 patient, and the FSH levels were uniformly elevated. Testosterone and LH were within normal limits except in 2 patients with slightly subnormal testosterone levels. 7 of the patients were married to women of fertile age, and in 3 cases the wife became pregnant and give birth to a healthy child. The time lapses from irradiation to conception were 18, 40 and 57 months. 2 of these patients had severe oligozoospermia on examination 2 and 4 months respectively from conception. Thus fertility may possibly be underestimated by sperm counting and hormone assays after this type of radiotherapy.", "contents": "Testicular function after radiotherapy to inverted 'Y' field for malignant lymphoma. Testicular function was estimated by sperm counts, hormone assays and recording of reported conceptions in 9 patients irradiated for malignant lymphoma. The treatment had been an inverted 'Y' field including the inguinal regions with, in addition, a mantle field in 8 patients. Azoospermia or severe oligozoospermia was found in all but 1 patient, and the FSH levels were uniformly elevated. Testosterone and LH were within normal limits except in 2 patients with slightly subnormal testosterone levels. 7 of the patients were married to women of fertile age, and in 3 cases the wife became pregnant and give birth to a healthy child. The time lapses from irradiation to conception were 18, 40 and 57 months. 2 of these patients had severe oligozoospermia on examination 2 and 4 months respectively from conception. Thus fertility may possibly be underestimated by sperm counting and hormone assays after this type of radiotherapy.", "PMID": 968446}
{"id": "pubmed23n0032_7291", "title": "Chromosome studies in untreated polycythaemia vera.", "content": "Bone marrow chromosomes were studied in a series of 50 consecutive, unselected cases of untreated polycythaemia vera (PV). A pathological cell line was present in 7 patients (= 14%). 3 patients each had 1 extra chromosome no. 8 and 1 extra no. 9. 2 patients had a similar but not identical marker chromosome including material from the long arms of chromosome no. 1. 1 of these patients also had a 20q- deletion. 1 patient had an extra unidentified isochromosome. 1 patient had a monosomy 16. In 5 cases the abnormal clone dominated over cells with normal karyotype. In each of 6 patients a singular hyperdiploid cell was observed. 1 or several polyploid or randomly hypodiploid cells were found in most patients. The findings in our material are compared to other reports of chromosome studies in PV and allied disorders. Except for the 20q- deletion no abnormality typical for PV has so far been detected, but some chromosomes seem to be involved more often than others (1, 8, 9, 20). Patients with chromosome aberrations did not show any clinical, laboratory or morphologic features which could separate them from cases with normal karyotypes. The prognostic significance of the chromosome abnormalities encountered in early PV cannot be defined at present.", "contents": "Chromosome studies in untreated polycythaemia vera. Bone marrow chromosomes were studied in a series of 50 consecutive, unselected cases of untreated polycythaemia vera (PV). A pathological cell line was present in 7 patients (= 14%). 3 patients each had 1 extra chromosome no. 8 and 1 extra no. 9. 2 patients had a similar but not identical marker chromosome including material from the long arms of chromosome no. 1. 1 of these patients also had a 20q- deletion. 1 patient had an extra unidentified isochromosome. 1 patient had a monosomy 16. In 5 cases the abnormal clone dominated over cells with normal karyotype. In each of 6 patients a singular hyperdiploid cell was observed. 1 or several polyploid or randomly hypodiploid cells were found in most patients. The findings in our material are compared to other reports of chromosome studies in PV and allied disorders. Except for the 20q- deletion no abnormality typical for PV has so far been detected, but some chromosomes seem to be involved more often than others (1, 8, 9, 20). Patients with chromosome aberrations did not show any clinical, laboratory or morphologic features which could separate them from cases with normal karyotypes. The prognostic significance of the chromosome abnormalities encountered in early PV cannot be defined at present.", "PMID": 968448}
{"id": "pubmed23n0032_7292", "title": "Chromosome abnormalities after chlorambucil therapy of polycythaemia vera.", "content": "Bone marrow chromosomes were studied in 2 patients with polycythaemia vera (PV) at the time of diagnosis and several times subsequently during therapy with chlorambucil. Both patients were normal in the initial study, but later developed pathological diploid cell lines with structural chromosome abnormalities. Case 1 presented after 25 months of myelosuppressive therapy a deletion of chromosome no. 12, del (12) (p11), in 60% of the diploid cells. 10 months later all studied cells in the marrow contained this deletion. In addition, the deletion of chromosome no. 20, described as characteristic for PV, was found in 3 cells. Case 2 demonstrated after 40 months on chlorambucil the presence of a balanced translocation, t (1;5) (p36;q31) in 90% of the cells. In none of the patients were any definite clinical, laboratory or morphological signs of an approaching leukaemic transformation of the polycythaemic disorder present.", "contents": "Chromosome abnormalities after chlorambucil therapy of polycythaemia vera. Bone marrow chromosomes were studied in 2 patients with polycythaemia vera (PV) at the time of diagnosis and several times subsequently during therapy with chlorambucil. Both patients were normal in the initial study, but later developed pathological diploid cell lines with structural chromosome abnormalities. Case 1 presented after 25 months of myelosuppressive therapy a deletion of chromosome no. 12, del (12) (p11), in 60% of the diploid cells. 10 months later all studied cells in the marrow contained this deletion. In addition, the deletion of chromosome no. 20, described as characteristic for PV, was found in 3 cells. Case 2 demonstrated after 40 months on chlorambucil the presence of a balanced translocation, t (1;5) (p36;q31) in 90% of the cells. In none of the patients were any definite clinical, laboratory or morphological signs of an approaching leukaemic transformation of the polycythaemic disorder present.", "PMID": 968449}
{"id": "pubmed23n0032_7293", "title": "Platelet function and plasma free fatty acids during acute myocardial infarction and severe angina pectoris.", "content": "The relationship between platelet function and plasma free fatty acid concentration has been studied serially during the initial 24 h in 11 patients suffering from acute myocardial infarction and in 5 patients with severe angina pectoris, Similar results were obtained in the 2 groups. Plasma free fatty acid concentration was initially high, and decreased significantly. The distribution of plasma free fatty acids remained unchanged. Platelet concentration was constant, whereas the percentage of reversible venous platelet aggregates initially was higher than in 11 healthy persons matched for age and sex. Platelet aggregates decreased transiently at 16 h. Venous reversible platelet aggregates correlated significantly with concentration of plasma free fatty acid, thus establishing a possible link between a change in lipid metabolism and platelet function. Plasma concentration of platelet factor 4 increased slightly but significantly during the initial hours. This may indicate an increased platelet release reaction.", "contents": "Platelet function and plasma free fatty acids during acute myocardial infarction and severe angina pectoris. The relationship between platelet function and plasma free fatty acid concentration has been studied serially during the initial 24 h in 11 patients suffering from acute myocardial infarction and in 5 patients with severe angina pectoris, Similar results were obtained in the 2 groups. Plasma free fatty acid concentration was initially high, and decreased significantly. The distribution of plasma free fatty acids remained unchanged. Platelet concentration was constant, whereas the percentage of reversible venous platelet aggregates initially was higher than in 11 healthy persons matched for age and sex. Platelet aggregates decreased transiently at 16 h. Venous reversible platelet aggregates correlated significantly with concentration of plasma free fatty acid, thus establishing a possible link between a change in lipid metabolism and platelet function. Plasma concentration of platelet factor 4 increased slightly but significantly during the initial hours. This may indicate an increased platelet release reaction.", "PMID": 968450}
{"id": "pubmed23n0032_7294", "title": "The peripheral platelet count in response to intravenous infusion of isoprenaline.", "content": "5 healthy male volunteers received i.v. infusions of isoprenaline in doses of 0.03, 0.06 and 0.09 mug X kg-1 X min-1 over a period of 6 min. A statistically significant decrease in the peripheral platelet count was obtained, but no dose-response-curve for isoprenaline was observed. It is suggested that while an i.v. infusion of epinephrine causes a release of platelets from the exchangeable splenic platelet pool, isoprenaline might have the opposite effect on the spleen, thereby causing a decrease of platelets in the peripheral blood.", "contents": "The peripheral platelet count in response to intravenous infusion of isoprenaline. 5 healthy male volunteers received i.v. infusions of isoprenaline in doses of 0.03, 0.06 and 0.09 mug X kg-1 X min-1 over a period of 6 min. A statistically significant decrease in the peripheral platelet count was obtained, but no dose-response-curve for isoprenaline was observed. It is suggested that while an i.v. infusion of epinephrine causes a release of platelets from the exchangeable splenic platelet pool, isoprenaline might have the opposite effect on the spleen, thereby causing a decrease of platelets in the peripheral blood.", "PMID": 968451}
{"id": "pubmed23n0032_7295", "title": "Fasting (acute energy deprivation) in man: effect on polymorphonuclear granulocyte functions, plasma iron and serum transferrin.", "content": "The effect of 10 days of total fasting (energy deprivation) on blood polymorphonuclear granulocyte functions, leukocyte numbers, iron and transferrin levels was evaluated in 14 healthy, normal-weight males. Granulocytes from 7 of the subjects were tested in vitro. A statistically significant depression was noted in their bactericidal capacity against Staph. aureus. The 14 subjects showed a marked decrease in the stainable activity of granulocyte alkaline phosphatase and decreases were noted in plasma iron and serum transferrin levels. The iron saturation of serum transferrin was unchanged. Thus, impairment of granulocyte bactericidal functions may occur secondarily to short-term total energy deprivation, in the absence of iron deficiency.", "contents": "Fasting (acute energy deprivation) in man: effect on polymorphonuclear granulocyte functions, plasma iron and serum transferrin. The effect of 10 days of total fasting (energy deprivation) on blood polymorphonuclear granulocyte functions, leukocyte numbers, iron and transferrin levels was evaluated in 14 healthy, normal-weight males. Granulocytes from 7 of the subjects were tested in vitro. A statistically significant depression was noted in their bactericidal capacity against Staph. aureus. The 14 subjects showed a marked decrease in the stainable activity of granulocyte alkaline phosphatase and decreases were noted in plasma iron and serum transferrin levels. The iron saturation of serum transferrin was unchanged. Thus, impairment of granulocyte bactericidal functions may occur secondarily to short-term total energy deprivation, in the absence of iron deficiency.", "PMID": 968452}
{"id": "pubmed23n0032_7296", "title": "Bone marrow metastases from prostatic cancer-marked cytolytic effect after only a few days of treatment with diethylstilbestrol.", "content": "A 42-year-old male patient became hospitalized with severe back pain and marked bleeding tendency from disseminated intravascular coagulation. The bone marrow aspirate showed numerous nests of cancer cells presumably from a prostatic carcinoma. After only 4 days of treatment with diethylstilbestrol his condition was markedly improved, and a new bone marrow aspirate showed extensive necrosis of the cancer cells.", "contents": "Bone marrow metastases from prostatic cancer-marked cytolytic effect after only a few days of treatment with diethylstilbestrol. A 42-year-old male patient became hospitalized with severe back pain and marked bleeding tendency from disseminated intravascular coagulation. The bone marrow aspirate showed numerous nests of cancer cells presumably from a prostatic carcinoma. After only 4 days of treatment with diethylstilbestrol his condition was markedly improved, and a new bone marrow aspirate showed extensive necrosis of the cancer cells.", "PMID": 968453}
{"id": "pubmed23n0032_7297", "title": "Unclassified haemolytic anaemia with splenomegaly and erythrocyte cation abnormalities--a disease of the spleen?", "content": "An unclassified case of haemolytic anaemia with voluminous splenomegaly is reported. This anaemia was normocytic without any specific morphologic aspect of red blood cells (RBC); Coombs test was negative; the osmotic fragility was normal; the increased autohaemolysis was not affected by the presence of glucose; Hb studies were normal; no RBC enzyme deficiency was found; RBC lipids and membrane proteins were normal; there was a marked reduction in RBC survival with exclusive splenic uptake of erythrocytes. Before splenectomy, RBC cations and water content were abnormal: 1) the RBC water was decreased moderately; 2) the RBC sodium was about twice the normal mean with an increased 22Na turn-over; 3) the RBC potassium was markedly reduced and 42K influx was twice the normal mean; 4) the RBC calcium content was increased. Splenectomy was followed by rapid disappearance of haemolysis and RBC water and cation disturbances. Because of this extremely rapid disappearance after splenectomy the authors suggest this case of haemolytic anaemia could be a primary disease of the spleen.", "contents": "Unclassified haemolytic anaemia with splenomegaly and erythrocyte cation abnormalities--a disease of the spleen? An unclassified case of haemolytic anaemia with voluminous splenomegaly is reported. This anaemia was normocytic without any specific morphologic aspect of red blood cells (RBC); Coombs test was negative; the osmotic fragility was normal; the increased autohaemolysis was not affected by the presence of glucose; Hb studies were normal; no RBC enzyme deficiency was found; RBC lipids and membrane proteins were normal; there was a marked reduction in RBC survival with exclusive splenic uptake of erythrocytes. Before splenectomy, RBC cations and water content were abnormal: 1) the RBC water was decreased moderately; 2) the RBC sodium was about twice the normal mean with an increased 22Na turn-over; 3) the RBC potassium was markedly reduced and 42K influx was twice the normal mean; 4) the RBC calcium content was increased. Splenectomy was followed by rapid disappearance of haemolysis and RBC water and cation disturbances. Because of this extremely rapid disappearance after splenectomy the authors suggest this case of haemolytic anaemia could be a primary disease of the spleen.", "PMID": 968454}
{"id": "pubmed23n0032_7298", "title": "Serial serum enzyme studies in infectious mononucleosis.", "content": "Serial examinations of the white cells in the blood and serial determinations of serum glutamic oxaloacetic transaminase (GOT) and serum lactic dehydrogenase (LDH) isoenzymes have been performed in 36 patients with seropositive infectious mononucleosis. The first blood sample taken after the onset of the disease showed an increased number of mononuclear cells in the blood in 90% of the cases, elevated GOT level in 70% and elevated LDH level in 100%. Mainly isoenzymes I, II and III accounted for the elevated LDH values, while only 35% of the patients had increased LDH V (liver fraction). The number of mononuclear cells in the blood was normalized in all patients after 5 weeks and GOT normalized after 10 weeks. On the other hand, half of the patients had increased LDH, particularly isoenzymes I, II, and III, 4 months after the onset of the disease. The results indicate that the liver involvement probably is slight and of short duration, while the proliferation of lymphoid cells in the tissues, probably responsible for the main part of the LDH increase, continues for a considerably longer period.", "contents": "Serial serum enzyme studies in infectious mononucleosis. Serial examinations of the white cells in the blood and serial determinations of serum glutamic oxaloacetic transaminase (GOT) and serum lactic dehydrogenase (LDH) isoenzymes have been performed in 36 patients with seropositive infectious mononucleosis. The first blood sample taken after the onset of the disease showed an increased number of mononuclear cells in the blood in 90% of the cases, elevated GOT level in 70% and elevated LDH level in 100%. Mainly isoenzymes I, II and III accounted for the elevated LDH values, while only 35% of the patients had increased LDH V (liver fraction). The number of mononuclear cells in the blood was normalized in all patients after 5 weeks and GOT normalized after 10 weeks. On the other hand, half of the patients had increased LDH, particularly isoenzymes I, II, and III, 4 months after the onset of the disease. The results indicate that the liver involvement probably is slight and of short duration, while the proliferation of lymphoid cells in the tissues, probably responsible for the main part of the LDH increase, continues for a considerably longer period.", "PMID": 968455}
{"id": "pubmed23n0032_7299", "title": "The dilemma of partially treated bacterial meningitis.", "content": "An analysis of 62 patients with pyogenic meningitis is presented, 23 (37%) of whom had received pre-diagnosis antibiotic therapy. Positive bacteriological identification could be achieved in 73% of the partially treated group as opposed to 97% in the previously untreated group but otherwise such pre-treatment made little impact on the diagnosis, characteristic cerebrospinal fluid changes sufficient for diagnostic purpose being present in all but one case. The study also fails to demonstrate any advantage of immunoelectroosmophoresis over conventional bacteriology in the problem cases of partially treated bacterial meningitis.", "contents": "The dilemma of partially treated bacterial meningitis. An analysis of 62 patients with pyogenic meningitis is presented, 23 (37%) of whom had received pre-diagnosis antibiotic therapy. Positive bacteriological identification could be achieved in 73% of the partially treated group as opposed to 97% in the previously untreated group but otherwise such pre-treatment made little impact on the diagnosis, characteristic cerebrospinal fluid changes sufficient for diagnostic purpose being present in all but one case. The study also fails to demonstrate any advantage of immunoelectroosmophoresis over conventional bacteriology in the problem cases of partially treated bacterial meningitis.", "PMID": 968456}
{"id": "pubmed23n0032_7300", "title": "The occurrence of human intestinal parasites in Finland.", "content": "A survey of the occurrence of intestinal parasites covered 243 inmates of institution for the mentally retarded, 537 hospital patients from 4 areas, 100 military servicemen, and 65 prisoners. Two faecal samples from each patient were examined by the formalin-ether concentration technique. Among the mentally retarded the prevalence of intestinal parasites was high, 41.3% among children and 51.5% among adults. The commonest parasite was Entamoeba coli. In Helsinki, the prevalence among children in hospital was only 7%, but among adult out patients, 18.9%. Among hospital patients in other areas, military servicemen, and prisoners in Helsinki the prevalence of intestinal parasites was low. The high prevalence of this protozoon, 14.8% among adult out-patients in Helsinki, probably reflects the increase in tourism. Four cases of Trichuris infection were detected among the mentally retarded.", "contents": "The occurrence of human intestinal parasites in Finland. A survey of the occurrence of intestinal parasites covered 243 inmates of institution for the mentally retarded, 537 hospital patients from 4 areas, 100 military servicemen, and 65 prisoners. Two faecal samples from each patient were examined by the formalin-ether concentration technique. Among the mentally retarded the prevalence of intestinal parasites was high, 41.3% among children and 51.5% among adults. The commonest parasite was Entamoeba coli. In Helsinki, the prevalence among children in hospital was only 7%, but among adult out patients, 18.9%. Among hospital patients in other areas, military servicemen, and prisoners in Helsinki the prevalence of intestinal parasites was low. The high prevalence of this protozoon, 14.8% among adult out-patients in Helsinki, probably reflects the increase in tourism. Four cases of Trichuris infection were detected among the mentally retarded.", "PMID": 968457}
{"id": "pubmed23n0032_7301", "title": "Gentamicin-induced nephropathy.", "content": "162 consecutive gentamicin courses have been evaluated retrospectively with respect to nephrotoxicity of gentamicin (GM). Of these, 120 courses were administered in 106 patients for more than 2 days and under adequate control of plasma creatinine (PCr). In 62 of these 120 courses, PCr concentrations increased. In 17 courses (14%), GM therapy was found to be the only demonstrable etiology to the rise in PCr. The 17 courses with GM-induced reduction in kidney function were characterized by a prolonged duration of treatment, a high total dose of GM and a somewhat higher level of serum GM than the 58 courses of GM treatment in which PCr remained unchanged. No significant differences were found with regard to age, average daily dose of GM, average daily dose per kg and average daily dose in proportion to average diuresis. Additional administration of other nephrotoxic drugs did not increase the incidence of GM-induced nephropathy. When GM was the only demonstrable cause of nephropathy, the elevation in PCr concentrations were generally mild and transient, while the nephropathy when other factors were involved more often became severe and occasionally irreversible.", "contents": "Gentamicin-induced nephropathy. 162 consecutive gentamicin courses have been evaluated retrospectively with respect to nephrotoxicity of gentamicin (GM). Of these, 120 courses were administered in 106 patients for more than 2 days and under adequate control of plasma creatinine (PCr). In 62 of these 120 courses, PCr concentrations increased. In 17 courses (14%), GM therapy was found to be the only demonstrable etiology to the rise in PCr. The 17 courses with GM-induced reduction in kidney function were characterized by a prolonged duration of treatment, a high total dose of GM and a somewhat higher level of serum GM than the 58 courses of GM treatment in which PCr remained unchanged. No significant differences were found with regard to age, average daily dose of GM, average daily dose per kg and average daily dose in proportion to average diuresis. Additional administration of other nephrotoxic drugs did not increase the incidence of GM-induced nephropathy. When GM was the only demonstrable cause of nephropathy, the elevation in PCr concentrations were generally mild and transient, while the nephropathy when other factors were involved more often became severe and occasionally irreversible.", "PMID": 968458}
{"id": "pubmed23n0032_7302", "title": "Interaction between cephaloridine and furosemide in man.", "content": "The effect of a moderate oral dose of furosemide (80 mg daily) on the serum half-life of cephaloridine was studied. In 14 patients not previously treated with diuretics this dose was found to prolong the half-life significantly. A corresponding decrease of the half-life of cephaloridine was observed when furosemide was withdrawn from 2 patients who were treated with diuretics at admission to hospital. The concurrent treatment with furosemide and cephaloridine did not affect the renal function of the patients studied. From the results obtained it seems possible that even moderate doses of a potent diuretic increase the nephrotoxicity of a cephalosporin.", "contents": "Interaction between cephaloridine and furosemide in man. The effect of a moderate oral dose of furosemide (80 mg daily) on the serum half-life of cephaloridine was studied. In 14 patients not previously treated with diuretics this dose was found to prolong the half-life significantly. A corresponding decrease of the half-life of cephaloridine was observed when furosemide was withdrawn from 2 patients who were treated with diuretics at admission to hospital. The concurrent treatment with furosemide and cephaloridine did not affect the renal function of the patients studied. From the results obtained it seems possible that even moderate doses of a potent diuretic increase the nephrotoxicity of a cephalosporin.", "PMID": 968459}
{"id": "pubmed23n0032_7303", "title": "Effect of foundry size on the dust concentration of different work phases.", "content": "The dust concentrations of different work phases in iron foundries of different sizes were studied. The results of the total dust measurements made during the Finnish Foundry Project were considered according to the eight main work phases, and the 51 iron foundries were divided into four groups according to the number of foundry workers. The division between the groups at 25, 50, and 100 workers is related to the degree of mechanization in Finnish foundries. The total dust concentration clearly increased in sand making and melting as the size of the foundry increased. The concentration decreased in molding, coremaking, knock-out, and cleaning as the number of workers increased. No significant differences between the foundry groups could be found during casting. The factors affecting the differences in dust concentrations are discussed.", "contents": "Effect of foundry size on the dust concentration of different work phases. The dust concentrations of different work phases in iron foundries of different sizes were studied. The results of the total dust measurements made during the Finnish Foundry Project were considered according to the eight main work phases, and the 51 iron foundries were divided into four groups according to the number of foundry workers. The division between the groups at 25, 50, and 100 workers is related to the degree of mechanization in Finnish foundries. The total dust concentration clearly increased in sand making and melting as the size of the foundry increased. The concentration decreased in molding, coremaking, knock-out, and cleaning as the number of workers increased. No significant differences between the foundry groups could be found during casting. The factors affecting the differences in dust concentrations are discussed.", "PMID": 968462}
{"id": "pubmed23n0032_7304", "title": "Carbon monoxide in foundry air.", "content": "The concentration of carbon monoxide in the air of 67 iron, steel, or copper alloy foundries using sand molding was measured. About 1,100 carbon monoxide determinations were made. High concentrations of carbon monoxide were found in the area around the cupolas and the casting sites in iron foundries. The blood carboxyhemoglobin levels of 145 workers from iron foundries were measured. The carboxyhemoglobin level of 6% was exceeded in 26% of the nonsmokers and in 71% of the smokers.", "contents": "Carbon monoxide in foundry air. The concentration of carbon monoxide in the air of 67 iron, steel, or copper alloy foundries using sand molding was measured. About 1,100 carbon monoxide determinations were made. High concentrations of carbon monoxide were found in the area around the cupolas and the casting sites in iron foundries. The blood carboxyhemoglobin levels of 145 workers from iron foundries were measured. The carboxyhemoglobin level of 6% was exceeded in 26% of the nonsmokers and in 71% of the smokers.", "PMID": 968463}
{"id": "pubmed23n0032_7305", "title": "Metal fumes in foundries.", "content": "The metal content of melting and casting fumes was analyzed with X-ray fluorescence, atomic absorption, and mass spectrometric methods. The composition of fumes varied with the kind of alloy, the quality of scrap, and the type of melting process. In addition foundry workers' exposure to metal fumes was evaluated. The measurements of airborne metal concentrations in 10 steel foundries, 15 iron foundries, and 11 copper alloy foundries showed that exposure to lead, copper, and zinc may present a health hazard.", "contents": "Metal fumes in foundries. The metal content of melting and casting fumes was analyzed with X-ray fluorescence, atomic absorption, and mass spectrometric methods. The composition of fumes varied with the kind of alloy, the quality of scrap, and the type of melting process. In addition foundry workers' exposure to metal fumes was evaluated. The measurements of airborne metal concentrations in 10 steel foundries, 15 iron foundries, and 11 copper alloy foundries showed that exposure to lead, copper, and zinc may present a health hazard.", "PMID": 968464}
{"id": "pubmed23n0032_7306", "title": "Gases formed from furan binding agents.", "content": "Furfuryl alcohol and formaldehyde concentrations in the air of coremaking areas of 10 iron and steel foundries were measured. The mean concentration of furfuryl alcohol and formaldehyde was 4.3 cm3/m3 and 2.7 cm3/m3, respectively. Furfuryl alcohol exceeded its threshold limit value (TLV) of 5 cm3/m3 in 22% of the determinations; and formaldehyde (TLV=2 cm3/m3) in 38%. In addition phenol concentration was determined in one foundry; and phosphoric acid concentration, in two foundries. The concentrations of phenol and phosphoric acid were far lower than their TLVs. The question is raised as to whether exposure to furfuryl alcohol and formaldehyde in foundries in combination with exposure to dust and smoking can increase the risk of chronic bronchitis.", "contents": "Gases formed from furan binding agents. Furfuryl alcohol and formaldehyde concentrations in the air of coremaking areas of 10 iron and steel foundries were measured. The mean concentration of furfuryl alcohol and formaldehyde was 4.3 cm3/m3 and 2.7 cm3/m3, respectively. Furfuryl alcohol exceeded its threshold limit value (TLV) of 5 cm3/m3 in 22% of the determinations; and formaldehyde (TLV=2 cm3/m3) in 38%. In addition phenol concentration was determined in one foundry; and phosphoric acid concentration, in two foundries. The concentrations of phenol and phosphoric acid were far lower than their TLVs. The question is raised as to whether exposure to furfuryl alcohol and formaldehyde in foundries in combination with exposure to dust and smoking can increase the risk of chronic bronchitis.", "PMID": 968465}
{"id": "pubmed23n0032_7307", "title": "Angina pectoris, ECG findings and blood pressure of foundry workers in relation to carbon monoxide exposure.", "content": "A prevalence study on angina pectoris, ECG changes, and blood pressure was carried out with those 1,000 workers (response rate 93.1%) with the longest exposure time (minimum 4.2 years) from a statistical sample of 20 foundries. The history of angina was obtained from a questionnaire recommended by the World Health Organization, and the ECGs were coded according to the Minnesota code. On the basis of measurements of the concentration of carbon monoxide (CO) in the air, the workers were divided into three occupational exposure groups, one with definite CO exposure, one with slight or occasional CO exposure, and one without CO exposure. Allowance was also made for present and past smoking habits. All comparisons were made on an intrastudy basis between the different subcategories. The prevalence of angina showed a clear dose-response relationship with regard to CO exposure from either occupation, smoking, or both, but no such trend was found for ECG findings suggestive of CHD. These results may suggest a greater sensitivity in detecting CHD on the part of the angina questionnaire. The systolic and diastolic blood pressures of CO exposed workers were slightly higher than those of other workers when age and smoking habits were taken into consideration. However, exposure to heat was intermixed with exposure to CO. It could be shown that selection based on health had operated in the foundries. Hence, the prevalence found can be considered to be underestimates of the \"true\" occurrence of cardiac disorders. Nevertheless both angina and \"coronary\" ECG findings were more prevalent than in other methodologically comparable studies on the general population or industrial workers without toxic exposure.", "contents": "Angina pectoris, ECG findings and blood pressure of foundry workers in relation to carbon monoxide exposure. A prevalence study on angina pectoris, ECG changes, and blood pressure was carried out with those 1,000 workers (response rate 93.1%) with the longest exposure time (minimum 4.2 years) from a statistical sample of 20 foundries. The history of angina was obtained from a questionnaire recommended by the World Health Organization, and the ECGs were coded according to the Minnesota code. On the basis of measurements of the concentration of carbon monoxide (CO) in the air, the workers were divided into three occupational exposure groups, one with definite CO exposure, one with slight or occasional CO exposure, and one without CO exposure. Allowance was also made for present and past smoking habits. All comparisons were made on an intrastudy basis between the different subcategories. The prevalence of angina showed a clear dose-response relationship with regard to CO exposure from either occupation, smoking, or both, but no such trend was found for ECG findings suggestive of CHD. These results may suggest a greater sensitivity in detecting CHD on the part of the angina questionnaire. The systolic and diastolic blood pressures of CO exposed workers were slightly higher than those of other workers when age and smoking habits were taken into consideration. However, exposure to heat was intermixed with exposure to CO. It could be shown that selection based on health had operated in the foundries. Hence, the prevalence found can be considered to be underestimates of the \"true\" occurrence of cardiac disorders. Nevertheless both angina and \"coronary\" ECG findings were more prevalent than in other methodologically comparable studies on the general population or industrial workers without toxic exposure.", "PMID": 968466}
{"id": "pubmed23n0032_7308", "title": "Prevalence of pneumoconiosis and chronic bronchitis in foundry workers.", "content": "The prevalence of pneumoconiosis, chronic bronchitis, and impaired lung function was studied among those 1,000 foundry workers (response rate 93.1%) with the longest exposure time (minimum 4.2, mean 17, SD 9 years) from a representative sample of 20 foundries. Pneumoconiosis was diagnosed from 100 x 100 mm radiographs, and the false positives and false negatives were evaluated from normal-size radiographs from all those with a positive finding and a sample of those with a negative finding. Chronic bronchitis was studied by means of a translation of the MRC Short Questionnarie on Respiratory Symptoms. Forced vital capacity and forced expiratory volume in 1 s were measured with a Vitalograph Single Breath Wedge Spirometer, and the FEV % was calculated from these variables. The subjects were grouped according to smoking habits and dust exposure, which could be fairly well evaluated from measurements performed in connection with the health survey. All comparisons were made between different subcategories. The overall prevalence of pneumoconiosis was 3.8%, when allowance had been made for false positive and false negative findings. Most cases were mild. Chronic bronchitis occurred more frequently among those occupied in jobs classified as dusty. Smoking also strongly increased its prevalence; a combination of both exposures produced the strongest effect. The effect of smoking was also evident as an impairment of lung function; however, no such effect of dust exposure could be shown in this material. Since this was a prevalence study, the selective removal of workers from dusty jobs probably led to underestimates of all the health effects studied. In spite of the effect of selection excess bronchitis could be demonstrated in workers from dusty environments. Therefore effective dust control must be initiated not only with regard to silica dust but also with respect to total dust.", "contents": "Prevalence of pneumoconiosis and chronic bronchitis in foundry workers. The prevalence of pneumoconiosis, chronic bronchitis, and impaired lung function was studied among those 1,000 foundry workers (response rate 93.1%) with the longest exposure time (minimum 4.2, mean 17, SD 9 years) from a representative sample of 20 foundries. Pneumoconiosis was diagnosed from 100 x 100 mm radiographs, and the false positives and false negatives were evaluated from normal-size radiographs from all those with a positive finding and a sample of those with a negative finding. Chronic bronchitis was studied by means of a translation of the MRC Short Questionnarie on Respiratory Symptoms. Forced vital capacity and forced expiratory volume in 1 s were measured with a Vitalograph Single Breath Wedge Spirometer, and the FEV % was calculated from these variables. The subjects were grouped according to smoking habits and dust exposure, which could be fairly well evaluated from measurements performed in connection with the health survey. All comparisons were made between different subcategories. The overall prevalence of pneumoconiosis was 3.8%, when allowance had been made for false positive and false negative findings. Most cases were mild. Chronic bronchitis occurred more frequently among those occupied in jobs classified as dusty. Smoking also strongly increased its prevalence; a combination of both exposures produced the strongest effect. The effect of smoking was also evident as an impairment of lung function; however, no such effect of dust exposure could be shown in this material. Since this was a prevalence study, the selective removal of workers from dusty jobs probably led to underestimates of all the health effects studied. In spite of the effect of selection excess bronchitis could be demonstrated in workers from dusty environments. Therefore effective dust control must be initiated not only with regard to silica dust but also with respect to total dust.", "PMID": 968467}
{"id": "pubmed23n0032_7309", "title": "A mortality study of foundry workers.", "content": "The mortality of foundry workers was studied from a sample of all those men employed in 20 representative iron, steel, and nonferrous foundries for any period of time during 1950 through 1972. A statistical sample of 3,876 men from all those 15,401 workers with at least 3 months' exposure formed the cohort under study. The actual number of person-years of follow-up became 47,160. Total and cause-specific mortality was studied in the entire cohort and in different categories based on exposure time and occupation. The foundry workers' experience was compared to that expected on the basis of the general male population's death rates in Finland, and different categories of the cohort were compared to each other through direct standardization. During the period from 1950 through 1973, there had occurred 224 deaths. The mortality approached the expected value computed from the age-adjusted general male population, the standardized mortality ratio (SMR) being 90 for all foundry workers and 95 for workers in \"typical\" foundry occupations. The corresponding standard mortality ratios based on the estimated total number of person-years, after the application of corrections for sampling fractions, were 86 and 95, respectively. There was a slight shift of the age of death towards younger age groups among the casters, fettlers, and furnace tenders. Mortality from coronary heart disease showed a standardized mortality ratio of 80 for the whole cohort; no significant differences were found for any occupational category. Lung cancer mortality was higher than expected (SMR 150) in the entire cohort; closer analysis revealed that the excess was confined to iron foundries, and especially to molders with more than 5 years of exposure. There were no more violent deaths than expected, not even from work accidents. Because most occupational cohorts have standardized mortality ratios that are well below 90, the present results were interpreted as probably indicating slightly elevated mortality. The most important finding was the concentration of lung cancer among molders in iron foundries.", "contents": "A mortality study of foundry workers. The mortality of foundry workers was studied from a sample of all those men employed in 20 representative iron, steel, and nonferrous foundries for any period of time during 1950 through 1972. A statistical sample of 3,876 men from all those 15,401 workers with at least 3 months' exposure formed the cohort under study. The actual number of person-years of follow-up became 47,160. Total and cause-specific mortality was studied in the entire cohort and in different categories based on exposure time and occupation. The foundry workers' experience was compared to that expected on the basis of the general male population's death rates in Finland, and different categories of the cohort were compared to each other through direct standardization. During the period from 1950 through 1973, there had occurred 224 deaths. The mortality approached the expected value computed from the age-adjusted general male population, the standardized mortality ratio (SMR) being 90 for all foundry workers and 95 for workers in \"typical\" foundry occupations. The corresponding standard mortality ratios based on the estimated total number of person-years, after the application of corrections for sampling fractions, were 86 and 95, respectively. There was a slight shift of the age of death towards younger age groups among the casters, fettlers, and furnace tenders. Mortality from coronary heart disease showed a standardized mortality ratio of 80 for the whole cohort; no significant differences were found for any occupational category. Lung cancer mortality was higher than expected (SMR 150) in the entire cohort; closer analysis revealed that the excess was confined to iron foundries, and especially to molders with more than 5 years of exposure. There were no more violent deaths than expected, not even from work accidents. Because most occupational cohorts have standardized mortality ratios that are well below 90, the present results were interpreted as probably indicating slightly elevated mortality. The most important finding was the concentration of lung cancer among molders in iron foundries.", "PMID": 968468}
{"id": "pubmed23n0032_7310", "title": "Turnover and health selection among foundry workers.", "content": "The quantity, reasons, and health selection involved in labor turnover were studied with the use of questionnaires and employers' records. The basic material was the personnel of 20 representative foundries. The turnover in 1950--1972 was estimated from a sample of 588 workers. The causes and health selection were studied with questionnaires put to the 1,789 current employees (91% response), the 493 foundrymen who had left after at least 5 years of exposure (the 5-year-plus men, 71% response) and 424 of those who had left after less than 1 year of exposure (the 1-year-minus men, 55% response). The men were asked to describe their present and earlier work at the foundry, the nature and duration of their exposure, diagnosed lung and heart diseases, and chronic bronchitis and angina pectoris and to assess their present and former state of health and work capacity. The disability analysis was based on a sample of 2,834 men whose data were taken from the Social Insurance Register. The disability findings were compared to expected values based on the Finnish male population. Turnover proved to be rapid; short periods of employment predominated. The major reasons for leaving were poor work conditions, physically demanding work, low pay, and poor health. The turnover was fastest in dusty occupations. Relatively more exfoundrymen, both 5-year-plus and 1-year-minus, than current employees felt their health and/or work capacity to be poor. More of the older men in the 5-year-plus group than men of the same age in the current group had chronic bronchitis and diagnosed lung disorders. Both the 5-year-plus and the 1-year-minus exfoundrymen had relatively more diagnosed heart disorders than did the current employees. The disability prevalences of the foundrymen in any category of diseases did not exceed the expected values based on the male population. The overall findings indicate early health selection prior to pensionable disability and/or death.", "contents": "Turnover and health selection among foundry workers. The quantity, reasons, and health selection involved in labor turnover were studied with the use of questionnaires and employers' records. The basic material was the personnel of 20 representative foundries. The turnover in 1950--1972 was estimated from a sample of 588 workers. The causes and health selection were studied with questionnaires put to the 1,789 current employees (91% response), the 493 foundrymen who had left after at least 5 years of exposure (the 5-year-plus men, 71% response) and 424 of those who had left after less than 1 year of exposure (the 1-year-minus men, 55% response). The men were asked to describe their present and earlier work at the foundry, the nature and duration of their exposure, diagnosed lung and heart diseases, and chronic bronchitis and angina pectoris and to assess their present and former state of health and work capacity. The disability analysis was based on a sample of 2,834 men whose data were taken from the Social Insurance Register. The disability findings were compared to expected values based on the Finnish male population. Turnover proved to be rapid; short periods of employment predominated. The major reasons for leaving were poor work conditions, physically demanding work, low pay, and poor health. The turnover was fastest in dusty occupations. Relatively more exfoundrymen, both 5-year-plus and 1-year-minus, than current employees felt their health and/or work capacity to be poor. More of the older men in the 5-year-plus group than men of the same age in the current group had chronic bronchitis and diagnosed lung disorders. Both the 5-year-plus and the 1-year-minus exfoundrymen had relatively more diagnosed heart disorders than did the current employees. The disability prevalences of the foundrymen in any category of diseases did not exceed the expected values based on the male population. The overall findings indicate early health selection prior to pensionable disability and/or death.", "PMID": 968469}
{"id": "pubmed23n0032_7311", "title": "Image modulation in corona discharge photography.", "content": "Photographic images obtained by the Kirlian technique are principally a record of corona activity during an exposure interval. Most of the variations in the images of the corona of a living subject who is in contact with the photographic film can be accounted for by the presence of moisture on or within the subject's surface. During exposure, moisture is transferred from the subject to the emulsion surface of the photographic film and causes an alteration of the electric charge pattern on the film, hence the electric field at the surface of the subject. As a result, large variations in the density of corona images, corona streamer trajectories, and image coloration can be brought about. The radial extent of corona images--that is, the range of corona streamers--is an inverse function of the resistance in the circuit formed by the high-voltage supply, the subject, and the film-electrode configuration. This is because the voltage at which corona is initiated is dependent on the rate of rise of the voltage impressed between the subject and the electrode, and the rate of rise is governed by the applied voltage waveform and the voltage drop across the resistance. The range of streamers is proportional to the corona onset voltage. However, we have not seen any influence of large changes in skin resistance on streamer range. Presumably, this is due to the shunting effect of skin capacitance. In general, the photographic response to moisture suggests that corona discharge photography may be useful in the detection and quantification of moisture in animate and inanimate specimens through the orderly modulation of the image due to various levels of moisture.", "contents": "Image modulation in corona discharge photography. Photographic images obtained by the Kirlian technique are principally a record of corona activity during an exposure interval. Most of the variations in the images of the corona of a living subject who is in contact with the photographic film can be accounted for by the presence of moisture on or within the subject's surface. During exposure, moisture is transferred from the subject to the emulsion surface of the photographic film and causes an alteration of the electric charge pattern on the film, hence the electric field at the surface of the subject. As a result, large variations in the density of corona images, corona streamer trajectories, and image coloration can be brought about. The radial extent of corona images--that is, the range of corona streamers--is an inverse function of the resistance in the circuit formed by the high-voltage supply, the subject, and the film-electrode configuration. This is because the voltage at which corona is initiated is dependent on the rate of rise of the voltage impressed between the subject and the electrode, and the rate of rise is governed by the applied voltage waveform and the voltage drop across the resistance. The range of streamers is proportional to the corona onset voltage. However, we have not seen any influence of large changes in skin resistance on streamer range. Presumably, this is due to the shunting effect of skin capacitance. In general, the photographic response to moisture suggests that corona discharge photography may be useful in the detection and quantification of moisture in animate and inanimate specimens through the orderly modulation of the image due to various levels of moisture.", "PMID": 968480}
{"id": "pubmed23n0032_7312", "title": "Picosecond chemistry.", "content": "The ability to directly measure and evaluate ultrafast processes with unprecedented time resolution and reliability has greatly extended our knowledge about the kinetics of primary processes in chemistry and allied physical and biological sciences. Improvements in the reliability and versatility of picosecond techniques should lead to an increase in the experimental information about basic interactions in atomic and molecular systems.", "contents": "Picosecond chemistry. The ability to directly measure and evaluate ultrafast processes with unprecedented time resolution and reliability has greatly extended our knowledge about the kinetics of primary processes in chemistry and allied physical and biological sciences. Improvements in the reliability and versatility of picosecond techniques should lead to an increase in the experimental information about basic interactions in atomic and molecular systems.", "PMID": 968481}
{"id": "pubmed23n0032_7313", "title": "Cooperative computation of stereo disparity.", "content": "The extraction of stereo-disparity information from two images depends upon establishing a correspondence between them. In this article we analyze the nature of the correspondence computation and derive a cooperative algorithm that implements it. We show that this algorithm successfully extracts information from random-dot stereograms, and its implications for the psychophysics and neurophysiology of the visual system are briefly discussed.", "contents": "Cooperative computation of stereo disparity. The extraction of stereo-disparity information from two images depends upon establishing a correspondence between them. In this article we analyze the nature of the correspondence computation and derive a cooperative algorithm that implements it. We show that this algorithm successfully extracts information from random-dot stereograms, and its implications for the psychophysics and neurophysiology of the visual system are briefly discussed.", "PMID": 968482}
{"id": "pubmed23n0032_7314", "title": "Bounds on \"bound water\": transverse nuclear magnetic resonance relaxation in barnacle muscle.", "content": "Relatively mobile protons that do not exchange with D2O exist in barnacle muscle cells. These are not part of the nonfreezing \"bound water\" that does exchange. Ninety-seven percent of the muscle water exhibits a single transverse relaxation time of 35 milliseconds: one water molecule per thousand, which is briefly and irrotationally bound, will produce the observed relaxation properties.", "contents": "Bounds on \"bound water\": transverse nuclear magnetic resonance relaxation in barnacle muscle. Relatively mobile protons that do not exchange with D2O exist in barnacle muscle cells. These are not part of the nonfreezing \"bound water\" that does exchange. Ninety-seven percent of the muscle water exhibits a single transverse relaxation time of 35 milliseconds: one water molecule per thousand, which is briefly and irrotationally bound, will produce the observed relaxation properties.", "PMID": 968484}
{"id": "pubmed23n0032_7315", "title": "(D-Ala2)-Met-enkephalinamide: a potent, long-lasting synthetic pentapeptide analgesic.", "content": "[D-Ala2]-Met-enkephalinamide (DALA), a synthetic enkephalin analog designed by in vitro analysis, binds to opiate receptors almost as tightly as methionine-enkephalin. Since it is not susceptible to degradation by brain enzymes, low doses (5 to 10 micrograms) cause profound, long-lasting, morphine-like analgesia when microinjected into rat brain.", "contents": "(D-Ala2)-Met-enkephalinamide: a potent, long-lasting synthetic pentapeptide analgesic. [D-Ala2]-Met-enkephalinamide (DALA), a synthetic enkephalin analog designed by in vitro analysis, binds to opiate receptors almost as tightly as methionine-enkephalin. Since it is not susceptible to degradation by brain enzymes, low doses (5 to 10 micrograms) cause profound, long-lasting, morphine-like analgesia when microinjected into rat brain.", "PMID": 968485}
{"id": "pubmed23n0032_7316", "title": "Genetic composition of a high-yielding influenza A virus recombinant: a vaccine strain against \"Swine\" influenza.", "content": "Analysis of the RNA migration pattern of a high-yielding influenza virus recombinant, X-53, used in vaccine production, reveals that only the two genes coding for hemagglutinin and neuraminidase antigens were derived from the \"swine\" influenza virus parent. A/New Jersey/11/76, while six were acquired from the A/PR/8/34 (HON1) parent, donor of the high yield characteristic.", "contents": "Genetic composition of a high-yielding influenza A virus recombinant: a vaccine strain against \"Swine\" influenza. Analysis of the RNA migration pattern of a high-yielding influenza virus recombinant, X-53, used in vaccine production, reveals that only the two genes coding for hemagglutinin and neuraminidase antigens were derived from the \"swine\" influenza virus parent. A/New Jersey/11/76, while six were acquired from the A/PR/8/34 (HON1) parent, donor of the high yield characteristic.", "PMID": 968486}
{"id": "pubmed23n0032_7317", "title": "Variations in writing posture and cerebral organization.", "content": "Two tachistoscopic tests of cerebral lateralization were administered to 73 subjects classified by handedness, sex, and hand orientation in writing. The results indicated that the direction of cerebral lateralization could be indexed from a subject's handedness and hand posture during writing. In subjects with a normal writing posture, the linguistically specialized hemisphere was contralateral to the dominant hand, and the visuospatially specialized hemisphere was ipsilateral; the reverse was true in subjects with an \"inverted\" hand position during writing. Females and subjects having an inverted hand posture manifested smaller degrees of lateral differentiation than males and subjects with a typical hand posture.", "contents": "Variations in writing posture and cerebral organization. Two tachistoscopic tests of cerebral lateralization were administered to 73 subjects classified by handedness, sex, and hand orientation in writing. The results indicated that the direction of cerebral lateralization could be indexed from a subject's handedness and hand posture during writing. In subjects with a normal writing posture, the linguistically specialized hemisphere was contralateral to the dominant hand, and the visuospatially specialized hemisphere was ipsilateral; the reverse was true in subjects with an \"inverted\" hand position during writing. Females and subjects having an inverted hand posture manifested smaller degrees of lateral differentiation than males and subjects with a typical hand posture.", "PMID": 968487}
{"id": "pubmed23n0032_7318", "title": "The biochemical high-risk paradigm: behavioral and familial correlates of low platelet monoamine oxidase activity.", "content": "A population of individuals potentially at risk for psychiatric disorders was identified by screening 375 college student volunteers for low platelet monoamine oxidase (MAO) activity levels. The lower and upper 10 per cent in MAO activity were interviewed and family history data were obtained. Low-MAO probands reported more frequent psychiatric or psychological counseling and problems with the law. Families of low MAO probands had an eightfold increase in the incidence of suicide or suicide attempts over those of high-MAO probands. This suggests that reduced MAO levels, reported previously in patients with affective disorders and chronic schizophrenia, may predict a vulnerability to psychiatric disorder.", "contents": "The biochemical high-risk paradigm: behavioral and familial correlates of low platelet monoamine oxidase activity. A population of individuals potentially at risk for psychiatric disorders was identified by screening 375 college student volunteers for low platelet monoamine oxidase (MAO) activity levels. The lower and upper 10 per cent in MAO activity were interviewed and family history data were obtained. Low-MAO probands reported more frequent psychiatric or psychological counseling and problems with the law. Families of low MAO probands had an eightfold increase in the incidence of suicide or suicide attempts over those of high-MAO probands. This suggests that reduced MAO levels, reported previously in patients with affective disorders and chronic schizophrenia, may predict a vulnerability to psychiatric disorder.", "PMID": 968488}
{"id": "pubmed23n0032_7319", "title": "St. Louis encephalitis: clinical and epidemiologic aspects, Mississippi, 1974.", "content": "During July, August, and September 1974, 23 confirmed or presumptive cases of St. Louis encephalitis occurred in Mississippi. Attack rates were highest in the northwest corner of the state and among persons over 70 years of age. Rural and urban residents had similar attack rates. Nine persons have not resumed their pre-illness level of activity because of residual neurologic impairment six months after their illness. Additional cases of St. Louis encephalitis are expected in the next few years.", "contents": "St. Louis encephalitis: clinical and epidemiologic aspects, Mississippi, 1974. During July, August, and September 1974, 23 confirmed or presumptive cases of St. Louis encephalitis occurred in Mississippi. Attack rates were highest in the northwest corner of the state and among persons over 70 years of age. Rural and urban residents had similar attack rates. Nine persons have not resumed their pre-illness level of activity because of residual neurologic impairment six months after their illness. Additional cases of St. Louis encephalitis are expected in the next few years.", "PMID": 968523}
{"id": "pubmed23n0032_7320", "title": "Mohs' chemosurgery and skin cancer.", "content": "Experience in treating 1, 275 skin cancers with Mohs' chemosurgery is presented. The Mohs technic uses microscopic examination to avoid excision of normal tissue and assure complete removal of the tumor. Chemosurgery is indicated for tumors which are poorly demarcated or which arise in areas known to have a high recurrence rate. It is the procedure of choice for almost all recurrent lesions.", "contents": "Mohs' chemosurgery and skin cancer. Experience in treating 1, 275 skin cancers with Mohs' chemosurgery is presented. The Mohs technic uses microscopic examination to avoid excision of normal tissue and assure complete removal of the tumor. Chemosurgery is indicated for tumors which are poorly demarcated or which arise in areas known to have a high recurrence rate. It is the procedure of choice for almost all recurrent lesions.", "PMID": 968524}
{"id": "pubmed23n0032_7321", "title": "Clinical comparison of isoflurance and halothane anesthetics.", "content": "Isoflurane and halothane were compared in two similar groups of 100 patients each. Isoflurane compared favorably with halothane in producing adequate anesthesia in all our patients. Induction period was a little stormy when there was direct induction with isoflurane. Maintenance was excellent and recovery was good. Mean concentration necessary to induce anesthesia was 3.07% with isoflurane and 2.56% with halothane. Mean maintenance with isoflurane was 1.39%, compared to 1.40% with halothane. Less curare was required for relaxation when used with isoflurane than with halothane. This difference was not seen with pancuronium (Pavulon). Patient recovery was faster with isoflurane than with halothane. Incidence of delirium and shivering in the recovery period was similar for both agents. Incidence of nausea and vomiting was greater with isoflurane. Other clinical and biochemical postoperative comparisons did not show any significant differences between the two agents.", "contents": "Clinical comparison of isoflurance and halothane anesthetics. Isoflurane and halothane were compared in two similar groups of 100 patients each. Isoflurane compared favorably with halothane in producing adequate anesthesia in all our patients. Induction period was a little stormy when there was direct induction with isoflurane. Maintenance was excellent and recovery was good. Mean concentration necessary to induce anesthesia was 3.07% with isoflurane and 2.56% with halothane. Mean maintenance with isoflurane was 1.39%, compared to 1.40% with halothane. Less curare was required for relaxation when used with isoflurane than with halothane. This difference was not seen with pancuronium (Pavulon). Patient recovery was faster with isoflurane than with halothane. Incidence of delirium and shivering in the recovery period was similar for both agents. Incidence of nausea and vomiting was greater with isoflurane. Other clinical and biochemical postoperative comparisons did not show any significant differences between the two agents.", "PMID": 968525}
{"id": "pubmed23n0032_7322", "title": "Pancreatic ascites.", "content": "Two patients with pancreatic ascites secondary to acute pancreatitis are presented. Successful drainage was carried out in one patient with distal pancreatectomy and Roux-en-Y pancreaticojejunostomy and in the other by cystoduodenostomy. Most cases of pancreatic ascites are due to a mild peritoneal reaction casued by inactivated pancreatic enzymes liberated after ductal or pseudocyst disruption. A majority of cases are successfully treated by surgical correction of the underlying pathology.", "contents": "Pancreatic ascites. Two patients with pancreatic ascites secondary to acute pancreatitis are presented. Successful drainage was carried out in one patient with distal pancreatectomy and Roux-en-Y pancreaticojejunostomy and in the other by cystoduodenostomy. Most cases of pancreatic ascites are due to a mild peritoneal reaction casued by inactivated pancreatic enzymes liberated after ductal or pseudocyst disruption. A majority of cases are successfully treated by surgical correction of the underlying pathology.", "PMID": 968526}
{"id": "pubmed23n0032_7323", "title": "Clinically significant hypercorticism secondary to local steroid therapy.", "content": "Multiple gastric ulcers developed secondary to systemic absorption of the topical synthetic steroid in this case. Rapid healing occurred as well as the resolution of the moon facies and hypertension on cessation of topical steroid administration and routine treatment for ulcers.", "contents": "Clinically significant hypercorticism secondary to local steroid therapy. Multiple gastric ulcers developed secondary to systemic absorption of the topical synthetic steroid in this case. Rapid healing occurred as well as the resolution of the moon facies and hypertension on cessation of topical steroid administration and routine treatment for ulcers.", "PMID": 968527}
{"id": "pubmed23n0032_7324", "title": "Adolescent genital dermatoses.", "content": "The female adolescent period rivals the menopausal years as a most tumultuous interval. Hormonal changes account for many dermatoses not seen in older women. Vulvar problems that commonly affect the adolescent are discussed in reference to the pathophysiology which may induce these changes. The hormonal milium at this time period is emphasized. A concise grouping of disease processes, as well as a description, clue to diagnosis, and treatment of each entity is presented.", "contents": "Adolescent genital dermatoses. The female adolescent period rivals the menopausal years as a most tumultuous interval. Hormonal changes account for many dermatoses not seen in older women. Vulvar problems that commonly affect the adolescent are discussed in reference to the pathophysiology which may induce these changes. The hormonal milium at this time period is emphasized. A concise grouping of disease processes, as well as a description, clue to diagnosis, and treatment of each entity is presented.", "PMID": 968528}
{"id": "pubmed23n0032_7325", "title": "Which colonic polyps should be excised endoscopically?", "content": "A review of 464 consecutive polypoid lesions of the colon reveals that virtually all pedunculated polyps and over 80% of 218 sessile polyps were removed colonoscopically. Although size or location of the lesions occasionally precludes colonoscopic excision, the endoscopic appearance of a sessile polyp is the most important factor in deciding upon the method of excision. In gneeral, smooth, soft, nonulcerated sessile lesions of all sizes were excised endoscopically, while approximately half of the larger (2-6 cm) firm, irregular-surfaced, benign sessile lesions, and all ulcerated or malignant sessile lesions required laparotomy for proper management. All polypoid lesions should be viewed endoscopically before deciding the method of excision, regardless of their size, location, or general appearnce by barium enema. Experience with endoscopic morphology and snare electrocautery technics was most important, since more than 85% of all polypoid lesions in this consecutive series were removed endoscopically.", "contents": "Which colonic polyps should be excised endoscopically? A review of 464 consecutive polypoid lesions of the colon reveals that virtually all pedunculated polyps and over 80% of 218 sessile polyps were removed colonoscopically. Although size or location of the lesions occasionally precludes colonoscopic excision, the endoscopic appearance of a sessile polyp is the most important factor in deciding upon the method of excision. In gneeral, smooth, soft, nonulcerated sessile lesions of all sizes were excised endoscopically, while approximately half of the larger (2-6 cm) firm, irregular-surfaced, benign sessile lesions, and all ulcerated or malignant sessile lesions required laparotomy for proper management. All polypoid lesions should be viewed endoscopically before deciding the method of excision, regardless of their size, location, or general appearnce by barium enema. Experience with endoscopic morphology and snare electrocautery technics was most important, since more than 85% of all polypoid lesions in this consecutive series were removed endoscopically.", "PMID": 968529}
{"id": "pubmed23n0032_7326", "title": "Pyogenic granuloma of the hand.", "content": "Pyogenic granuloma is a benign, vascular lesion occasionally seen on the hand. It many be quite alarming to the patient. Adequate surgical excision is advocated as the treatment of choice.", "contents": "Pyogenic granuloma of the hand. Pyogenic granuloma is a benign, vascular lesion occasionally seen on the hand. It many be quite alarming to the patient. Adequate surgical excision is advocated as the treatment of choice.", "PMID": 968530}
{"id": "pubmed23n0032_7327", "title": "Infective endocarditis as a complication of heroin use.", "content": "Infective endocarditis in heroin addicts has been reported to have a mortality as high as 85% and reports have varied widely regarding predominant valvular involvement and infecting microorganisms. A retrspective study was done and 61 cases of heroin-associated infective endocarditis were identified at Freedmen's Hospital and the District of Columbia General Hospital, Washington, DC between January 1969 and January 1973. Our results indicate that staphylococcal infection of the tricuspid valve has a much higher incidence in this population than has generally been believed and that it is the predominant presentation of infective endocarditis in these patients. The outcome of patients in our series compares favorably with previous reports and suggests that early diagnosis and prompt institution of appropriate antimicrobial therapy are important and may lead to improved survival in addicts with tricuspid endocarditis.", "contents": "Infective endocarditis as a complication of heroin use. Infective endocarditis in heroin addicts has been reported to have a mortality as high as 85% and reports have varied widely regarding predominant valvular involvement and infecting microorganisms. A retrspective study was done and 61 cases of heroin-associated infective endocarditis were identified at Freedmen's Hospital and the District of Columbia General Hospital, Washington, DC between January 1969 and January 1973. Our results indicate that staphylococcal infection of the tricuspid valve has a much higher incidence in this population than has generally been believed and that it is the predominant presentation of infective endocarditis in these patients. The outcome of patients in our series compares favorably with previous reports and suggests that early diagnosis and prompt institution of appropriate antimicrobial therapy are important and may lead to improved survival in addicts with tricuspid endocarditis.", "PMID": 968531}
{"id": "pubmed23n0032_7328", "title": "Culture, adaptation, and patterns of disease.", "content": "The sick patient, rather than the disease process itself, should occupy the central focus of the physician's attention. Such an approach recognizes the interaction of physiologic and emotional forces in the occurrence of disease. It allows for a consideration of the contributory role of emotions in the precipition of physical illness and a recognition that physical illness may produce emotional sequelae of relevance to the total clinical problem. It is an approach which views man as an adapting animal, whose environment may play a crucial role in his adaptation and his disease. Only with such a perspective of man and disease can the principles of medical treatment be realistic.", "contents": "Culture, adaptation, and patterns of disease. The sick patient, rather than the disease process itself, should occupy the central focus of the physician's attention. Such an approach recognizes the interaction of physiologic and emotional forces in the occurrence of disease. It allows for a consideration of the contributory role of emotions in the precipition of physical illness and a recognition that physical illness may produce emotional sequelae of relevance to the total clinical problem. It is an approach which views man as an adapting animal, whose environment may play a crucial role in his adaptation and his disease. Only with such a perspective of man and disease can the principles of medical treatment be realistic.", "PMID": 968532}
{"id": "pubmed23n0032_7329", "title": "Appendictiis in pregnancy.", "content": "Twenty-nine cases of acute appendicitis during pregnancy are presented, and the recent literature is reviewed. Occurring once in 1,600 gestations, appendicitis carries a pregnancy loss of 10.8%. Difficulties in diagnosis and delays in treatment increase with advancing gestational age. Aggressive management may minimize the resultant maternal morbidity and fetal mortality.", "contents": "Appendictiis in pregnancy. Twenty-nine cases of acute appendicitis during pregnancy are presented, and the recent literature is reviewed. Occurring once in 1,600 gestations, appendicitis carries a pregnancy loss of 10.8%. Difficulties in diagnosis and delays in treatment increase with advancing gestational age. Aggressive management may minimize the resultant maternal morbidity and fetal mortality.", "PMID": 968533}
{"id": "pubmed23n0032_7330", "title": "Reconstruction of the oral cavity after obliterative surgery for cancer.", "content": "Simple and reliable methods for reconstruction of the oral cavity after obliterative surgery for carcinoma include (1) nasolabial cheek flaps, (2) hemiforehead and total forehead flaps, and (3) temporal island flaps. In general, the nasolabial flap is sufficient for resurfacing areas of the oral cavity anterior to the second molar tooth, while the temporal island flap provides satisfactory coverage posterior to this area. The forehead flap, which is very durable and reliable, is reserved for reconstruction of the more massive defects. Technics and use of each reconstructive procedure are described.", "contents": "Reconstruction of the oral cavity after obliterative surgery for cancer. Simple and reliable methods for reconstruction of the oral cavity after obliterative surgery for carcinoma include (1) nasolabial cheek flaps, (2) hemiforehead and total forehead flaps, and (3) temporal island flaps. In general, the nasolabial flap is sufficient for resurfacing areas of the oral cavity anterior to the second molar tooth, while the temporal island flap provides satisfactory coverage posterior to this area. The forehead flap, which is very durable and reliable, is reserved for reconstruction of the more massive defects. Technics and use of each reconstructive procedure are described.", "PMID": 968534}
{"id": "pubmed23n0032_7331", "title": "Brief treatment of sexual problems.", "content": "Brief, behaviorally oriented treatment of sexual dysfunction is gaining increasing acceptance in medicine and psychiatry. However, relatively few clinics have published their treatment results. During a 2 1/2-year period, 36 couples with diagnoses of sexual dysfunction were treated with a modified Masters and Johnson protocol. The initial success rate was 83%, but the degree of improvement reported by the couples varied from marked to equivocal. Eighty-nine percent of the male partners and 83% of the female partners manifested psychologic signs or symptoms that either warranted a psychiatric diagnosis or were noted as significant traits or symptoms. With an average of 7.9 months of follow-up, the failure rate increased from 17% to 38%. This increase was attributed in part to the degree of combined psychosexual disorder identified in the patients. The etiologic relationships between psychiatric conditions and sexual dysfunctions are not fully understood and will require further study.", "contents": "Brief treatment of sexual problems. Brief, behaviorally oriented treatment of sexual dysfunction is gaining increasing acceptance in medicine and psychiatry. However, relatively few clinics have published their treatment results. During a 2 1/2-year period, 36 couples with diagnoses of sexual dysfunction were treated with a modified Masters and Johnson protocol. The initial success rate was 83%, but the degree of improvement reported by the couples varied from marked to equivocal. Eighty-nine percent of the male partners and 83% of the female partners manifested psychologic signs or symptoms that either warranted a psychiatric diagnosis or were noted as significant traits or symptoms. With an average of 7.9 months of follow-up, the failure rate increased from 17% to 38%. This increase was attributed in part to the degree of combined psychosexual disorder identified in the patients. The etiologic relationships between psychiatric conditions and sexual dysfunctions are not fully understood and will require further study.", "PMID": 968535}
{"id": "pubmed23n0032_7332", "title": "Cardiac valve replacement in the first 21 years of life.", "content": "From 1969 through 1974, 25 patients under age 21 years had prosthetic cardiac vlave replacement at the Emory University Hospital. Twenty-nine valves were replaced in this group. Patients ranged in age from 2 to 21 years, with a mean of 14 years. Concomitant correction of associated cardiac lesions was required in 14 (56%). Five (21%) had had prior open valvuoplastic procedures. Operative mortality for the entire group was 12%, with an additional late mortality of 8%. There have been no early or late deaths in the last three years' experience. Best results are obtained when early correction of isolated valvular lesions is done as soon as clinical deterioration on vigorous medical management is recognized. The presence of multiple intrcardiac lesions or prior valve surgery does not preclude excellent results. Current indications for surgical correction and follow-up management are presented.", "contents": "Cardiac valve replacement in the first 21 years of life. From 1969 through 1974, 25 patients under age 21 years had prosthetic cardiac vlave replacement at the Emory University Hospital. Twenty-nine valves were replaced in this group. Patients ranged in age from 2 to 21 years, with a mean of 14 years. Concomitant correction of associated cardiac lesions was required in 14 (56%). Five (21%) had had prior open valvuoplastic procedures. Operative mortality for the entire group was 12%, with an additional late mortality of 8%. There have been no early or late deaths in the last three years' experience. Best results are obtained when early correction of isolated valvular lesions is done as soon as clinical deterioration on vigorous medical management is recognized. The presence of multiple intrcardiac lesions or prior valve surgery does not preclude excellent results. Current indications for surgical correction and follow-up management are presented.", "PMID": 968536}
{"id": "pubmed23n0032_7333", "title": "Histologic factors affecting prognosis of pure seminoma of the testis.", "content": "The histologic features of 92 cases of pure seminoma of the testis were reviewed in relation to the presence and degree of fibrosis, necrosis, granulomatous and lymphocytic reactions, interstitial cell hyperplasia, and lymphatic and/or vascular invasion associated with the primary tumor. No relationship between any of the histologic factors studied and survival was established.", "contents": "Histologic factors affecting prognosis of pure seminoma of the testis. The histologic features of 92 cases of pure seminoma of the testis were reviewed in relation to the presence and degree of fibrosis, necrosis, granulomatous and lymphocytic reactions, interstitial cell hyperplasia, and lymphatic and/or vascular invasion associated with the primary tumor. No relationship between any of the histologic factors studied and survival was established.", "PMID": 968537}
{"id": "pubmed23n0032_7334", "title": "Early surgical managment of penetrating ocular injuries involving the posterior segment.", "content": "Pars plana vitrectomy technic can be used in the early management of certain penetrating ocular injuries involving the posterior segment, including selected intraocular foreign bodies. This study reports the results of ten consecutive cases of intraocular foreign bodies in the posterior segment treated by a combination of vitrectomy (including lensectomy when necessary) and foreign-body extraction with forceps. The foreign body was successfully removed in nine of ten eyes, and nine of ten eyes were salvaged. This favorable experience using early vitreous surgery suggests that the vitrectomy technic can be used in other penetrating injuries involving the posterior segment that are not associated with intraocular foreign bodies. Possible indications for early vitrectomy are presented, including cases with a poor prognosis when managed by conventional methods.", "contents": "Early surgical managment of penetrating ocular injuries involving the posterior segment. Pars plana vitrectomy technic can be used in the early management of certain penetrating ocular injuries involving the posterior segment, including selected intraocular foreign bodies. This study reports the results of ten consecutive cases of intraocular foreign bodies in the posterior segment treated by a combination of vitrectomy (including lensectomy when necessary) and foreign-body extraction with forceps. The foreign body was successfully removed in nine of ten eyes, and nine of ten eyes were salvaged. This favorable experience using early vitreous surgery suggests that the vitrectomy technic can be used in other penetrating injuries involving the posterior segment that are not associated with intraocular foreign bodies. Possible indications for early vitrectomy are presented, including cases with a poor prognosis when managed by conventional methods.", "PMID": 968538}
{"id": "pubmed23n0032_7335", "title": "Immunology of the lung.", "content": "Recent conceptual advances in immunology are reviewed. Although immune reactions take place in all organs, those occurring in the lung were chosen for illustrative purposes. The lung's enormous surface area has direct contact with the external environment through the airways and receives all of the cardiac output, a situation which exposes it to numerous, potentially harmful agents. The four basic types of immune responses are depicted as ending with tissue injury, but in most instances these reactions protect the host from disease. Manipulation of the immune system with transfer factor, new routes of immunization with existing vaccines, and other forms of immunotherapy are potential avenues for future management of disease.", "contents": "Immunology of the lung. Recent conceptual advances in immunology are reviewed. Although immune reactions take place in all organs, those occurring in the lung were chosen for illustrative purposes. The lung's enormous surface area has direct contact with the external environment through the airways and receives all of the cardiac output, a situation which exposes it to numerous, potentially harmful agents. The four basic types of immune responses are depicted as ending with tissue injury, but in most instances these reactions protect the host from disease. Manipulation of the immune system with transfer factor, new routes of immunization with existing vaccines, and other forms of immunotherapy are potential avenues for future management of disease.", "PMID": 968539}
{"id": "pubmed23n0032_7336", "title": "Pregnancy complicated by Cushing's syndrome.", "content": "A case of Cushing's syndrome occurring during the eighth month of pregnancy was presented. Steroid values and the results of dexamethasone suppression tests were markedly abnormal. Urinary steroid excretion reverted toward normal following delivery and prior to the removal of a large adrenal cortical adenoma.", "contents": "Pregnancy complicated by Cushing's syndrome. A case of Cushing's syndrome occurring during the eighth month of pregnancy was presented. Steroid values and the results of dexamethasone suppression tests were markedly abnormal. Urinary steroid excretion reverted toward normal following delivery and prior to the removal of a large adrenal cortical adenoma.", "PMID": 968541}
{"id": "pubmed23n0032_7337", "title": "Intramedullary lipoma of the distal femur.", "content": "A rare case of intramedullary lipoma involving the distal femur is presented and discussed in relation to previously reported cases. The 26 previously reported extracranial cases of this bone lesion are briefly reviewed. Emphasis is placed upon establishing a definitive pathologic diagnosis by open biopsy when one is dealing with a large bony lesion that has indefinite radiologic features.", "contents": "Intramedullary lipoma of the distal femur. A rare case of intramedullary lipoma involving the distal femur is presented and discussed in relation to previously reported cases. The 26 previously reported extracranial cases of this bone lesion are briefly reviewed. Emphasis is placed upon establishing a definitive pathologic diagnosis by open biopsy when one is dealing with a large bony lesion that has indefinite radiologic features.", "PMID": 968542}
{"id": "pubmed23n0032_7338", "title": "Respiratory insufficiency after intraperitoneal administration of Kanamycin: failure of calcium gluconate to reverse toxic effects.", "content": "A 47-year-old woman developed postanesthesia respiratory insufficiency five hours after an intramuscular injection of 0.5 gm of kanamycin and one hour and 40 minutes after peritoneal lavage with 1.0 gm of kanamycin. The respiratory insufficiency was not reversed by an intravenous infusion of 400 mg of calcium gluconate. This is the third reported instance of respiratory insufficiency after kanamycin lavage. Only one of the previously reported patients responded to intravenous infusion of calcium gluconate, despite the experimental evidence supporting its efficacy.", "contents": "Respiratory insufficiency after intraperitoneal administration of Kanamycin: failure of calcium gluconate to reverse toxic effects. A 47-year-old woman developed postanesthesia respiratory insufficiency five hours after an intramuscular injection of 0.5 gm of kanamycin and one hour and 40 minutes after peritoneal lavage with 1.0 gm of kanamycin. The respiratory insufficiency was not reversed by an intravenous infusion of 400 mg of calcium gluconate. This is the third reported instance of respiratory insufficiency after kanamycin lavage. Only one of the previously reported patients responded to intravenous infusion of calcium gluconate, despite the experimental evidence supporting its efficacy.", "PMID": 968543}
{"id": "pubmed23n0032_7339", "title": "Massive intraperitoneal hemorrhage from ruptured corpus luteum during anticoagulant therapy.", "content": "The unpredictability of hemorrhagic complications during anticoagulant therapy is well known. An unusual case of massive intraperitoneal hemorrhage from a ruptured corpus luteum during such therapy is presented. That this complication is uncommon may be  because relatively few premenopausal women are placed on anticoagulant therapy. In addition, the most likely condition for which premenopausal women are given anticoagulants is thrombophlebitis associated with pregnancy and childbirth when ovulation is inhibited.", "contents": "Massive intraperitoneal hemorrhage from ruptured corpus luteum during anticoagulant therapy. The unpredictability of hemorrhagic complications during anticoagulant therapy is well known. An unusual case of massive intraperitoneal hemorrhage from a ruptured corpus luteum during such therapy is presented. That this complication is uncommon may be  because relatively few premenopausal women are placed on anticoagulant therapy. In addition, the most likely condition for which premenopausal women are given anticoagulants is thrombophlebitis associated with pregnancy and childbirth when ovulation is inhibited.", "PMID": 968544}
{"id": "pubmed23n0032_7340", "title": "Congenital absence of the left pericardium: an unusual palpation finding and echocardiographic demonstration of the defect.", "content": "A patient with classical clinical and roentgenographic features of congenital absence of the left pericardium is described. On physical examination in the supine position, the precordial apical impluse was markedly displaced laterally. In the left lateral decubitus position, however, the apical impulse was felt in a mor medial location. This apparently paradoxical movement of the apical impulse was not found in persons with normal hearts or with left ventricular enlargement of hypertrophy, and may be characteristic of the pericardial defect. Further, with echocardiography we were able to demonstrate the absence of a pericardial echo on the lateral surface of the heart. Thus, echocardiography may be a useful noninvasive tool in the diagnosis of this defect.", "contents": "Congenital absence of the left pericardium: an unusual palpation finding and echocardiographic demonstration of the defect. A patient with classical clinical and roentgenographic features of congenital absence of the left pericardium is described. On physical examination in the supine position, the precordial apical impluse was markedly displaced laterally. In the left lateral decubitus position, however, the apical impulse was felt in a mor medial location. This apparently paradoxical movement of the apical impulse was not found in persons with normal hearts or with left ventricular enlargement of hypertrophy, and may be characteristic of the pericardial defect. Further, with echocardiography we were able to demonstrate the absence of a pericardial echo on the lateral surface of the heart. Thus, echocardiography may be a useful noninvasive tool in the diagnosis of this defect.", "PMID": 968545}
{"id": "pubmed23n0032_7341", "title": "Traumatic rupture of right diaphragm presenting as pleuropneumonia.", "content": "We present a case of rupture of the right diaphragm, due to an automobile accident, discovered after a period of eight years and presenting a clinical picture of pleuropneumonia. The radiologic appearances of traumatic hernia of the diaphragm are more difficult to appreciate on the right side because of the presence of the liver and delayed symptoms. This may be dangerous or sometimes fatal.", "contents": "Traumatic rupture of right diaphragm presenting as pleuropneumonia. We present a case of rupture of the right diaphragm, due to an automobile accident, discovered after a period of eight years and presenting a clinical picture of pleuropneumonia. The radiologic appearances of traumatic hernia of the diaphragm are more difficult to appreciate on the right side because of the presence of the liver and delayed symptoms. This may be dangerous or sometimes fatal.", "PMID": 968546}
{"id": "pubmed23n0032_7342", "title": "Tracheobronchiomegaly (Mounier-Kuhn syndrome).", "content": "Tracheobronchiomegaly is a rare congenital disorder due to defective development of elastic and muscle tissues in the upper airways. The characteristic radiologic features shown by bronchography include marked dilatation of the tracheobronchial system and diffuse saccular bronchiectasis. These findings and recurrent episodes of pneumonia lead to a progressive loss of pulmonary function. Even with vigorous medical therapy the prognosis is poor and severe respiratory failure eventually develops.", "contents": "Tracheobronchiomegaly (Mounier-Kuhn syndrome). Tracheobronchiomegaly is a rare congenital disorder due to defective development of elastic and muscle tissues in the upper airways. The characteristic radiologic features shown by bronchography include marked dilatation of the tracheobronchial system and diffuse saccular bronchiectasis. These findings and recurrent episodes of pneumonia lead to a progressive loss of pulmonary function. Even with vigorous medical therapy the prognosis is poor and severe respiratory failure eventually develops.", "PMID": 968547}
{"id": "pubmed23n0032_7343", "title": "Recovery from malignant hypertension with anuria after prolonged hemodialysis.", "content": "A 29-year-old black woman with malignant hypertension and anuria was treated with hemodialysis and antihypertensive drugs. Her renal function improved and her creatinine clearance was 28 ml/min after 4 1/2 months of treatment. Similar cases in the literature are reviewed.", "contents": "Recovery from malignant hypertension with anuria after prolonged hemodialysis. A 29-year-old black woman with malignant hypertension and anuria was treated with hemodialysis and antihypertensive drugs. Her renal function improved and her creatinine clearance was 28 ml/min after 4 1/2 months of treatment. Similar cases in the literature are reviewed.", "PMID": 968548}
{"id": "pubmed23n0032_7344", "title": "Acute acquired toxoplasmosis.", "content": "A case of acute acquired toxoplasmosis presenting as a fever of unknown origin is described in a 62-year-old man. The diagnosis was prompted by the presence of Toxoplasma gondii in lymph nodes obtained at abdominal laparotomy. The unusual features of the case include the patient's age, the presence of cysts in the lymph node, and itc clinical presentation as a prolonged remittent fever.", "contents": "Acute acquired toxoplasmosis. A case of acute acquired toxoplasmosis presenting as a fever of unknown origin is described in a 62-year-old man. The diagnosis was prompted by the presence of Toxoplasma gondii in lymph nodes obtained at abdominal laparotomy. The unusual features of the case include the patient's age, the presence of cysts in the lymph node, and itc clinical presentation as a prolonged remittent fever.", "PMID": 968549}
{"id": "pubmed23n0032_7345", "title": "Homozygous beta thalassemia in a black adult: follow-up after two decases.", "content": "A 54-year-old woman, perhaps the oldest living American black with homozygous beta thalassemia, has had a relatively benign clinical course since her case was first reported two decades ago. Despite progressive splenomegaly, she has remained active without blood transfusions. This case emphasizes the mildness of homozygous beta thalassemia in black subject.", "contents": "Homozygous beta thalassemia in a black adult: follow-up after two decases. A 54-year-old woman, perhaps the oldest living American black with homozygous beta thalassemia, has had a relatively benign clinical course since her case was first reported two decades ago. Despite progressive splenomegaly, she has remained active without blood transfusions. This case emphasizes the mildness of homozygous beta thalassemia in black subject.", "PMID": 968550}
{"id": "pubmed23n0032_7346", "title": "Vitamin E-dependent anemia in a premature infant.", "content": "Reported is a case of a premature infant who developed a well documented hemolytic anemia which responded to vitamin E therapy. The infant developed the syndrome while receiving an artificial formula containing iron and vitamin E, plus iron supplementation. The infant had a feeding problem which may have complicated absorption of vitamin E. It is suggested that premature infants who are formula fed should not receive iron supplement until they have doubled their birth weight or have a hemoglobin concentration of less than 10 mg%. Premature infants should receive supplemental vitamin E if they are not breast fed.", "contents": "Vitamin E-dependent anemia in a premature infant. Reported is a case of a premature infant who developed a well documented hemolytic anemia which responded to vitamin E therapy. The infant developed the syndrome while receiving an artificial formula containing iron and vitamin E, plus iron supplementation. The infant had a feeding problem which may have complicated absorption of vitamin E. It is suggested that premature infants who are formula fed should not receive iron supplement until they have doubled their birth weight or have a hemoglobin concentration of less than 10 mg%. Premature infants should receive supplemental vitamin E if they are not breast fed.", "PMID": 968551}
{"id": "pubmed23n0032_7347", "title": "Prosthetic valve endocarditis due to Mycobacterium chelonei.", "content": "Starr-Edwards mitral and aortic prosthetic valves served as nidi for endocarditis due to M chelonei. Cultural, physiologic, lipid chromatographic, and serologic studies identified the organism as M chelonei (abscessus).", "contents": "Prosthetic valve endocarditis due to Mycobacterium chelonei. Starr-Edwards mitral and aortic prosthetic valves served as nidi for endocarditis due to M chelonei. Cultural, physiologic, lipid chromatographic, and serologic studies identified the organism as M chelonei (abscessus).", "PMID": 968552}
{"id": "pubmed23n0032_7348", "title": "Effects of breast-feeding practice on the post-partum endometrium.", "content": "Inactive endometrial patterns occur frequently in the post-partum period and their occurrence is modified by the breast-feeding practice. An abnormal endometrium pattern, viz., cystic hyperplasia, may occur more frequently in non-lactating women and in patients using oestrogens, especially long-acting preparations, for lactation suppression. Post-ovulatory endometrial patterns in lactating women occur infrequently during the puerperium. The implications of these findings are discussed.", "contents": "Effects of breast-feeding practice on the post-partum endometrium. Inactive endometrial patterns occur frequently in the post-partum period and their occurrence is modified by the breast-feeding practice. An abnormal endometrium pattern, viz., cystic hyperplasia, may occur more frequently in non-lactating women and in patients using oestrogens, especially long-acting preparations, for lactation suppression. Post-ovulatory endometrial patterns in lactating women occur infrequently during the puerperium. The implications of these findings are discussed.", "PMID": 968653}
{"id": "pubmed23n0032_7349", "title": "The alleviation of uterocornual spasm of the Fallopian tubes during hysterosalpingography by intravenous administration of orciprenaline.", "content": "A simple, safe and reliable method of differentiating between organic obstruction spasm at the uterocornual junction of the oviduct during routine hysterosalpingography is described. Seventeen patients were studied. In all patients uterocornual obstruction was found during hysterosalpingography, which was performed without general anaesthesia. In 8 patients the apparent obstruction was alleviated within 30 seconds of the intravenous administration of 0.25 mg orciprenaline. Surgical and endoscopic findings confirmed the presence of the obstruction in all the other 9 patients. These findings are discussed on the basis of the neuro-anatomy of the Fallopian tube. It is suggested that this form of treatment, in many cases, eliminates the need to perform the procedure under general anaesthesia, which is the only other method of consistently alleviating such a 'spasm'. The use of orciprenaline may facilitate a rapid turnover of patients in units where hysterosalpingography is performed as a screening investigation for infertility. Furthermore, it is suggested that the oral administration of beta-adrenergic agents during the peri-ovulatory period to patients in whom 'tubal spasm' has been diagnosed might offer a rational approach to treatment.", "contents": "The alleviation of uterocornual spasm of the Fallopian tubes during hysterosalpingography by intravenous administration of orciprenaline. A simple, safe and reliable method of differentiating between organic obstruction spasm at the uterocornual junction of the oviduct during routine hysterosalpingography is described. Seventeen patients were studied. In all patients uterocornual obstruction was found during hysterosalpingography, which was performed without general anaesthesia. In 8 patients the apparent obstruction was alleviated within 30 seconds of the intravenous administration of 0.25 mg orciprenaline. Surgical and endoscopic findings confirmed the presence of the obstruction in all the other 9 patients. These findings are discussed on the basis of the neuro-anatomy of the Fallopian tube. It is suggested that this form of treatment, in many cases, eliminates the need to perform the procedure under general anaesthesia, which is the only other method of consistently alleviating such a 'spasm'. The use of orciprenaline may facilitate a rapid turnover of patients in units where hysterosalpingography is performed as a screening investigation for infertility. Furthermore, it is suggested that the oral administration of beta-adrenergic agents during the peri-ovulatory period to patients in whom 'tubal spasm' has been diagnosed might offer a rational approach to treatment.", "PMID": 968660}
{"id": "pubmed23n0032_7350", "title": "Fetal biparietal diameter and birth weight. An interpopulation comparison.", "content": "Data on the biparietal diameter (BPD), determined ultrasonically, of the full-term South African Negroid fetus are presented. Population differences in the absolute value of the BPD and the linear regression of birth weight on the biparietal dimension are emphasised. The possible biological explanations for these differences are discussed.", "contents": "Fetal biparietal diameter and birth weight. An interpopulation comparison. Data on the biparietal diameter (BPD), determined ultrasonically, of the full-term South African Negroid fetus are presented. Population differences in the absolute value of the BPD and the linear regression of birth weight on the biparietal dimension are emphasised. The possible biological explanations for these differences are discussed.", "PMID": 968661}
{"id": "pubmed23n0032_7351", "title": "The significance of serum oestradiol and progesterone levels in evaluating the response to clomiphene citrate.", "content": "In practice, the prediction of ovulation after the administration of clomiphene citrate (Clomid) has been based on measurement of biphasic basal body temperature and menstrual changes. In this article a protocol is presented to gauge adequately the extent of the response to the drug and to monitor reliably the occurrence of ovulation. The measurement of oestradiol levels at two different times, coupled with a single progesterone estimation, has proved the method of choice in monitoring Clomid response. These assays may provide reliable criteria for establishing suitable doses for individual patients.", "contents": "The significance of serum oestradiol and progesterone levels in evaluating the response to clomiphene citrate. In practice, the prediction of ovulation after the administration of clomiphene citrate (Clomid) has been based on measurement of biphasic basal body temperature and menstrual changes. In this article a protocol is presented to gauge adequately the extent of the response to the drug and to monitor reliably the occurrence of ovulation. The measurement of oestradiol levels at two different times, coupled with a single progesterone estimation, has proved the method of choice in monitoring Clomid response. These assays may provide reliable criteria for establishing suitable doses for individual patients.", "PMID": 968662}
{"id": "pubmed23n0032_7352", "title": "A comparison of pregnancy tests.", "content": "An evaluation in terms of performance and cost was made of the Planotest, Pregnex, Gravindex, Prepurex, Planosec, Quick Test and Dap Test pregnancy test kits. It was found that Pregnex was the most accurate, but that it was expensive. Planotest, Gravindex and Prepurex were identical as far as results were concerned and they were comparable in price. In terms of readability, Gravindex, Pregnex and Prepurex were considered the easiest. Because many pregnancy test kits are available, we thought it desirable to compare these kits in terms of cost and performance.", "contents": "A comparison of pregnancy tests. An evaluation in terms of performance and cost was made of the Planotest, Pregnex, Gravindex, Prepurex, Planosec, Quick Test and Dap Test pregnancy test kits. It was found that Pregnex was the most accurate, but that it was expensive. Planotest, Gravindex and Prepurex were identical as far as results were concerned and they were comparable in price. In terms of readability, Gravindex, Pregnex and Prepurex were considered the easiest. Because many pregnancy test kits are available, we thought it desirable to compare these kits in terms of cost and performance.", "PMID": 968663}
{"id": "pubmed23n0032_7353", "title": "Pregnancy after autotransplantation of the Fallopian tube in the ewe.", "content": "Vascularised transplantation of the Fallopian tube is a technically feasible operation, but its functional result remains questionable. This article documents a pregnancy after vascularised contralateral autotransplantation of the oviduct in a ewe.", "contents": "Pregnancy after autotransplantation of the Fallopian tube in the ewe. Vascularised transplantation of the Fallopian tube is a technically feasible operation, but its functional result remains questionable. This article documents a pregnancy after vascularised contralateral autotransplantation of the oviduct in a ewe.", "PMID": 968664}
{"id": "pubmed23n0032_7354", "title": "Ultrasonography in obstetric and gynaecological private practice.", "content": "A resume of the main uses of echography in obstetrics and gynaecology in a private practice is presented. The procedure is a simple, atraumatic one, without danger of X-radiation to the fetus or the hazard of angiography to the patient, and can be repeated without risk. It has a high degree of accuracy, as well as being a very useful aid, to gynaecologists and general practitioners alike, whenever there is doubt in the clinical assessment, or for confirmation of any underlying complication.", "contents": "Ultrasonography in obstetric and gynaecological private practice. A resume of the main uses of echography in obstetrics and gynaecology in a private practice is presented. The procedure is a simple, atraumatic one, without danger of X-radiation to the fetus or the hazard of angiography to the patient, and can be repeated without risk. It has a high degree of accuracy, as well as being a very useful aid, to gynaecologists and general practitioners alike, whenever there is doubt in the clinical assessment, or for confirmation of any underlying complication.", "PMID": 968665}
{"id": "pubmed23n0032_7355", "title": "Structural abnormalities of muscle tissue in ankylosing spondylitis.", "content": "Muscle tissue of patients with ankylosing spondylitis has been studied by means of histology, histochemistry and electron microscopy and has been shown to be grossly abnormal. The underlying basis of the muscle changes is probably neuropathic and we believe that these changes form part of the over-all pathology of this disease.", "contents": "Structural abnormalities of muscle tissue in ankylosing spondylitis. Muscle tissue of patients with ankylosing spondylitis has been studied by means of histology, histochemistry and electron microscopy and has been shown to be grossly abnormal. The underlying basis of the muscle changes is probably neuropathic and we believe that these changes form part of the over-all pathology of this disease.", "PMID": 968669}
{"id": "pubmed23n0032_7356", "title": "Primary hyperparathyroidism in Black South Africans.", "content": "Despite the presence of stimuli to parathyroid activity, such as low dietary intake of calcium and low serum calcium levels, primary hyperparathyroidism is extremely uncommon in the indigenous population of Africa south of the Sahara. We report the occurrence of primary hyperparathyroidism in 3 black patients, all of whom presented with gross bone disease, high serum calcium levels, short histories of hyperparathyroidism and large parathyroid tumours. The clinical manifestations of hyperparathyroidism in these patients are probably due to the rapid rate of tumour growth and high level of hormone production.", "contents": "Primary hyperparathyroidism in Black South Africans. Despite the presence of stimuli to parathyroid activity, such as low dietary intake of calcium and low serum calcium levels, primary hyperparathyroidism is extremely uncommon in the indigenous population of Africa south of the Sahara. We report the occurrence of primary hyperparathyroidism in 3 black patients, all of whom presented with gross bone disease, high serum calcium levels, short histories of hyperparathyroidism and large parathyroid tumours. The clinical manifestations of hyperparathyroidism in these patients are probably due to the rapid rate of tumour growth and high level of hormone production.", "PMID": 968670}
{"id": "pubmed23n0032_7357", "title": "Total electrical alternans in a patient with tuberculous pericarditis.", "content": "Total electrical alternans is an uncommon electrocardiographic abnormality in which alternation in the direction and/or amplitude of all the components (P, QRS and T) of the tracing is present. The phenomenon is seen only in association with occasional cases of pericaditis with large effusion's. Knowledge of this fact may help in the often difficult differentiation between a pericardial effusion and cardiomyopathy. An illustrative case report is presented, with a r\u00e9sum\u00e9 of the mechanisms postulated to explain total electrical alternans.", "contents": "Total electrical alternans in a patient with tuberculous pericarditis. Total electrical alternans is an uncommon electrocardiographic abnormality in which alternation in the direction and/or amplitude of all the components (P, QRS and T) of the tracing is present. The phenomenon is seen only in association with occasional cases of pericaditis with large effusion's. Knowledge of this fact may help in the often difficult differentiation between a pericardial effusion and cardiomyopathy. An illustrative case report is presented, with a r\u00e9sum\u00e9 of the mechanisms postulated to explain total electrical alternans.", "PMID": 968671}
{"id": "pubmed23n0032_7358", "title": "Some problems in the hospital management of criminal mental patients.", "content": "In view of impending new legislation in Rhodesia, a survey of 256 criminal mental patients admitted to Ingutsheni Hospital was undertaken. These patients were all treated by the author and were admitted during the 5-year period 1 January 1970-31 December 1974. Among these patients there were 113 schizophrenics (44%) and 57 epileptics (22%); these proportions are not significantly different from those previously found among civil patients. The outcome was that 86 patients (33, 6%) were discharged to stand trial, 107 (41, 8%) were discharged in terms of a recommendation by the Mental Hospital Board and 57 (22, 3%) were returned to prison. Recommendations are made for the provision of a new forensic psychiatric unit, and some of its functions are discussed.", "contents": "Some problems in the hospital management of criminal mental patients. In view of impending new legislation in Rhodesia, a survey of 256 criminal mental patients admitted to Ingutsheni Hospital was undertaken. These patients were all treated by the author and were admitted during the 5-year period 1 January 1970-31 December 1974. Among these patients there were 113 schizophrenics (44%) and 57 epileptics (22%); these proportions are not significantly different from those previously found among civil patients. The outcome was that 86 patients (33, 6%) were discharged to stand trial, 107 (41, 8%) were discharged in terms of a recommendation by the Mental Hospital Board and 57 (22, 3%) were returned to prison. Recommendations are made for the provision of a new forensic psychiatric unit, and some of its functions are discussed.", "PMID": 968672}
{"id": "pubmed23n0032_7359", "title": "The clinical assessment of ageing and problems of diagnosis in the elderly.", "content": "The elderly patient presents special problems to the clinician. Accurate diagnosis in the elderly requires an understanding of the interrelationship between mental and physical illness, the altered physiology of the aged and the realisation that multiple pathology is the rule rather than the exception. The onset of illness is frequently insidious and the presentation often atypical.", "contents": "The clinical assessment of ageing and problems of diagnosis in the elderly. The elderly patient presents special problems to the clinician. Accurate diagnosis in the elderly requires an understanding of the interrelationship between mental and physical illness, the altered physiology of the aged and the realisation that multiple pathology is the rule rather than the exception. The onset of illness is frequently insidious and the presentation often atypical.", "PMID": 968673}
{"id": "pubmed23n0032_7360", "title": "The doctor's problems.", "content": "A problem is defined and its purpose is described. The doctor's personality is analysed in its various aspects, and the patient and his complaints are dealt with. Their relationship reveals their respective needs and the scheme presented is an attempt to find a satisfactory solution to the multiple problems of human existence.", "contents": "The doctor's problems. A problem is defined and its purpose is described. The doctor's personality is analysed in its various aspects, and the patient and his complaints are dealt with. Their relationship reveals their respective needs and the scheme presented is an attempt to find a satisfactory solution to the multiple problems of human existence.", "PMID": 968674}
{"id": "pubmed23n0032_7361", "title": "[Pancytopenia and aplastic anemia: a retrospective study].", "content": "During January 1971--June 1975 we examined 195 patients with pancytopenia. The cause was bone marrow failure in 67.7% of cases (classic aplastic anaemia in 11.3%) hypersplenism in 7.7%, massive blood transfusions in 1.5%, severe infections in 9.7% (Gram-negative in 3%), and various other conditions in 7.8%. Records were insufficient for diagnosis in 5.6% of cases. Analysis of the 22 patients with aplastic anaemia showed no apparent aetiology in 16 (72.7%), previous phenylbutazone ingestion in 2, and Fanconi-type anaemia in 4 of 7 children. One-year survival was 73.7%, 2-year survival 71.4%, 3-year survival 63.6% and 4-year survival 57.1%. Marrow-investigation of the 21 available samples showed that 6 were acellular, 11 hypocellular and 4 normocellular. All patients received at least temporary therapy with anabolic steroids but its effectivity could not be satisfactorily assessed. Five patients died within 7 months and 5 patients went into remission, needing no further therapy. The initial haematological features of the 5 patients who died were not significantly different from those of the rest of the patients.", "contents": "[Pancytopenia and aplastic anemia: a retrospective study]. During January 1971--June 1975 we examined 195 patients with pancytopenia. The cause was bone marrow failure in 67.7% of cases (classic aplastic anaemia in 11.3%) hypersplenism in 7.7%, massive blood transfusions in 1.5%, severe infections in 9.7% (Gram-negative in 3%), and various other conditions in 7.8%. Records were insufficient for diagnosis in 5.6% of cases. Analysis of the 22 patients with aplastic anaemia showed no apparent aetiology in 16 (72.7%), previous phenylbutazone ingestion in 2, and Fanconi-type anaemia in 4 of 7 children. One-year survival was 73.7%, 2-year survival 71.4%, 3-year survival 63.6% and 4-year survival 57.1%. Marrow-investigation of the 21 available samples showed that 6 were acellular, 11 hypocellular and 4 normocellular. All patients received at least temporary therapy with anabolic steroids but its effectivity could not be satisfactorily assessed. Five patients died within 7 months and 5 patients went into remission, needing no further therapy. The initial haematological features of the 5 patients who died were not significantly different from those of the rest of the patients.", "PMID": 968678}
{"id": "pubmed23n0032_7362", "title": "The Peutz-Jeghers syndrome: a report of 3 cases.", "content": "Three cases of the Peutz-Jeghers syndrome are reported and the literature is reviewed. The difficulties in the management of these patients are described and the importance of radiology and endoscopy is emphasised.", "contents": "The Peutz-Jeghers syndrome: a report of 3 cases. Three cases of the Peutz-Jeghers syndrome are reported and the literature is reviewed. The difficulties in the management of these patients are described and the importance of radiology and endoscopy is emphasised.", "PMID": 968679}
{"id": "pubmed23n0032_7363", "title": "Treatment of frigidity in general practice.", "content": "The commonest causes of female sexual frigidity in general practice are outlined. Thirty-four couples were interviewed, and an approach to female frigidity is explained, which can be used by the general practitioner.", "contents": "Treatment of frigidity in general practice. The commonest causes of female sexual frigidity in general practice are outlined. Thirty-four couples were interviewed, and an approach to female frigidity is explained, which can be used by the general practitioner.", "PMID": 968680}
{"id": "pubmed23n0032_7364", "title": "A review of colonoscopy in 200 patients.", "content": "During the examination of the colon by fibre-optic colonoscopy in 200 consecutive patients it was possible to reach the caecum or suspected lesion in 80% of cases. Careful bowel preparation, sedation and detailed attention to techniques described are necessary for safe and successful performance of this procedure. In 85% of examinations, colonoscopy produced positive results including the definition of uncertain radiological lesions in 45 patients, finding of a cause for rectal bleeding not shown on barium studies in 15 patients, the assessment of inflammatory bowel disease in 28 patients, and the removal of polyps in 40 patients. Important was the finding of a normal colon in 49 cases, which obviated unnecessary surgery. Colonic perforation occurred in 2 patients. The limitations and complications of the procedure must be realised, but it is concluded that the colonoscope provides a valuable and effective means of diagnosis and therapy of lesions in the large bowel.", "contents": "A review of colonoscopy in 200 patients. During the examination of the colon by fibre-optic colonoscopy in 200 consecutive patients it was possible to reach the caecum or suspected lesion in 80% of cases. Careful bowel preparation, sedation and detailed attention to techniques described are necessary for safe and successful performance of this procedure. In 85% of examinations, colonoscopy produced positive results including the definition of uncertain radiological lesions in 45 patients, finding of a cause for rectal bleeding not shown on barium studies in 15 patients, the assessment of inflammatory bowel disease in 28 patients, and the removal of polyps in 40 patients. Important was the finding of a normal colon in 49 cases, which obviated unnecessary surgery. Colonic perforation occurred in 2 patients. The limitations and complications of the procedure must be realised, but it is concluded that the colonoscope provides a valuable and effective means of diagnosis and therapy of lesions in the large bowel.", "PMID": 968685}
{"id": "pubmed23n0032_7365", "title": "Buccal administration of fenoterol aerosol in young children with asthma.", "content": "A new technique for the administration of a bronchodilator aerosol, fenoterol, in young children with asthma is described. Rapid and effective bronchodilatation can be obtained in many patients simply by directing the jet of the bronchodilator aerosol onto the buccal mucosa. This method will frequently obviate the need for painful or potentially dangerous injections of adrenaline or aminophylline as a first-line treatment in children with acute attacks of asthma.", "contents": "Buccal administration of fenoterol aerosol in young children with asthma. A new technique for the administration of a bronchodilator aerosol, fenoterol, in young children with asthma is described. Rapid and effective bronchodilatation can be obtained in many patients simply by directing the jet of the bronchodilator aerosol onto the buccal mucosa. This method will frequently obviate the need for painful or potentially dangerous injections of adrenaline or aminophylline as a first-line treatment in children with acute attacks of asthma.", "PMID": 968686}
{"id": "pubmed23n0032_7366", "title": "A necropsy study of diabetes mellitus in natal blacks.", "content": "During a period of 10 years (1965 - 1974) necropsies on 126 Black diabetic patients were performed at King Edward VIII Hospital, Durban. Metabolic factors accounted for 39% of deaths and infection for 34% of all deaths in diabetes mellitus. Sixty-two per cent of the patients who died were below the age of 55 years. These findings are similar to those pertaining in the pre-insulin era.", "contents": "A necropsy study of diabetes mellitus in natal blacks. During a period of 10 years (1965 - 1974) necropsies on 126 Black diabetic patients were performed at King Edward VIII Hospital, Durban. Metabolic factors accounted for 39% of deaths and infection for 34% of all deaths in diabetes mellitus. Sixty-two per cent of the patients who died were below the age of 55 years. These findings are similar to those pertaining in the pre-insulin era.", "PMID": 968687}
{"id": "pubmed23n0032_7367", "title": "Combined liver-lung gamma imaging in subphrenic abscess.", "content": "Combined scintigraphy of the lung and liver provides a sensitive, simple and non-invasive method of evaluating the subphrenic space. Since the surgical approach is different in subphrenic, subhepatic and intrahepatic abscesses, accurate localisation and recognition of single or multiple abscesses provides the basis for an operative approach which may avoid unnecessary violation and contamination of the peritoneal cavity. The importance of correlation of the scintiscans with companion roentgenograms of the abdomen and chest, and accurate localisation of the diaphragm using serial scanning, is emphasised. Negative good-quality scans reliably rule out subphrenic abscess.", "contents": "Combined liver-lung gamma imaging in subphrenic abscess. Combined scintigraphy of the lung and liver provides a sensitive, simple and non-invasive method of evaluating the subphrenic space. Since the surgical approach is different in subphrenic, subhepatic and intrahepatic abscesses, accurate localisation and recognition of single or multiple abscesses provides the basis for an operative approach which may avoid unnecessary violation and contamination of the peritoneal cavity. The importance of correlation of the scintiscans with companion roentgenograms of the abdomen and chest, and accurate localisation of the diaphragm using serial scanning, is emphasised. Negative good-quality scans reliably rule out subphrenic abscess.", "PMID": 968688}
{"id": "pubmed23n0032_7368", "title": "Lead encephalopathy: case reports.", "content": "Six cases of lead encephalopathy in Black children are described. There was a delay in the diagnosis of the first 4 cases, partly owing to a lack of awareness of the possibility of exposure to lead in this population. The importance of the safe disposal of industrial wastes is stressed.", "contents": "Lead encephalopathy: case reports. Six cases of lead encephalopathy in Black children are described. There was a delay in the diagnosis of the first 4 cases, partly owing to a lack of awareness of the possibility of exposure to lead in this population. The importance of the safe disposal of industrial wastes is stressed.", "PMID": 968689}
{"id": "pubmed23n0032_7369", "title": "Pseudopneumoperitoneum: a case report.", "content": "A case of post-traumatic pseudocyst of the pancreas is presented. The elevated and compressed stomach showed an appearance on X-ray film which might be interpreted as free peritoneal air. The term pseudopneumoperitoneum is defined and other causes of this condition are mentioned.", "contents": "Pseudopneumoperitoneum: a case report. A case of post-traumatic pseudocyst of the pancreas is presented. The elevated and compressed stomach showed an appearance on X-ray film which might be interpreted as free peritoneal air. The term pseudopneumoperitoneum is defined and other causes of this condition are mentioned.", "PMID": 968692}
{"id": "pubmed23n0032_7370", "title": "Dilation of the upper urinary tract in pregnancy: a case report.", "content": "A case is presented in which the upper moiety of the pelvicalyceal system and ureter in unilateral duplication was spared the dilatation which affected the lower moiety and opposite upper urinary tract during pregnancy.", "contents": "Dilation of the upper urinary tract in pregnancy: a case report. A case is presented in which the upper moiety of the pelvicalyceal system and ureter in unilateral duplication was spared the dilatation which affected the lower moiety and opposite upper urinary tract during pregnancy.", "PMID": 968693}
{"id": "pubmed23n0032_7371", "title": "Computerised axial tomography of the head.", "content": "The technique and applicaton of computerised axial tomography of the head is briefly reviewed with regard to both normal and some pathological tomographic features.", "contents": "Computerised axial tomography of the head. The technique and applicaton of computerised axial tomography of the head is briefly reviewed with regard to both normal and some pathological tomographic features.", "PMID": 968694}
{"id": "pubmed23n0032_7372", "title": "Cranial and intracranial tuberculosis.", "content": "The radiographic appearances of cranial and intracranial tuberculous lesions are reviewed. The manifestations of cranial and intracranial tuberculosis vary according to the location, severity of involvement and the chronicity of the disease.", "contents": "Cranial and intracranial tuberculosis. The radiographic appearances of cranial and intracranial tuberculous lesions are reviewed. The manifestations of cranial and intracranial tuberculosis vary according to the location, severity of involvement and the chronicity of the disease.", "PMID": 968695}
{"id": "pubmed23n0032_7373", "title": "Syringomyelia secondary to paraplegia due to fractures of the thoracic spine.", "content": "The clinical, radiological and operative findings in 3 patients with paraplegia consequent upon fractures of the thoracic spine are presented. Myelography revealed widening of the cervical spinal cord in all 3 patients and this finding was confirmed at operation. The incidence of this condition in the Spinal Injuries Unit is shown to be similar to that noted in other large units. The principal theories as to the aetiology of this condition, i.e. cavitation secondary to haematomyelia or myelomalacia caused by circulatory disorders, are reviewed.", "contents": "Syringomyelia secondary to paraplegia due to fractures of the thoracic spine. The clinical, radiological and operative findings in 3 patients with paraplegia consequent upon fractures of the thoracic spine are presented. Myelography revealed widening of the cervical spinal cord in all 3 patients and this finding was confirmed at operation. The incidence of this condition in the Spinal Injuries Unit is shown to be similar to that noted in other large units. The principal theories as to the aetiology of this condition, i.e. cavitation secondary to haematomyelia or myelomalacia caused by circulatory disorders, are reviewed.", "PMID": 968696}
{"id": "pubmed23n0032_7374", "title": "Microsurgical removal of acoustic neuromas.", "content": "This article reviews the selection and use of instruments for the microsurgical removal of acoustic neuromas by the suboccipital-transmeatal approach. The accurate use of carefully selected instruments for perineural dissection when combined with the use of the operating microscope can lead to frequent preservation of the facial nerve and occasional retention of hearing.", "contents": "Microsurgical removal of acoustic neuromas. This article reviews the selection and use of instruments for the microsurgical removal of acoustic neuromas by the suboccipital-transmeatal approach. The accurate use of carefully selected instruments for perineural dissection when combined with the use of the operating microscope can lead to frequent preservation of the facial nerve and occasional retention of hearing.", "PMID": 968719}
{"id": "pubmed23n0032_7375", "title": "A technique for early Pantopaque myelography in cervical spinal cord injuries.", "content": "A simple, reliable method for early Pantopaque myelography in patients with traumatic injuries of the cervical spinal cord is presented. Myelography is accomplished with the patient prone on a Stryker frame after vertebral reduction and alignment have been achieved with axial skeletal traction. A lateral C1-2 spinal puncture and a portable \"\"C-arm'' image intensifier with televised fluoroscopy are used. There have been no complications and no occurrences of vertebral re-dislocation with this technique.", "contents": "A technique for early Pantopaque myelography in cervical spinal cord injuries. A simple, reliable method for early Pantopaque myelography in patients with traumatic injuries of the cervical spinal cord is presented. Myelography is accomplished with the patient prone on a Stryker frame after vertebral reduction and alignment have been achieved with axial skeletal traction. A lateral C1-2 spinal puncture and a portable \"\"C-arm'' image intensifier with televised fluoroscopy are used. There have been no complications and no occurrences of vertebral re-dislocation with this technique.", "PMID": 968721}
{"id": "pubmed23n0032_7376", "title": "Reversal of experimental cerebral vasospasm by intravenous nitroprusside therapy.", "content": "Cerebral vasospasm was induced in dogs by intracisternal injection of blood. After angiographic demonstration of spasm, sodium nitroprusside was infused intravenously and its effect on the diameter of the basilar artery was studied angiographically. Ten experiments were performed within 90 minutes of the induction of spasm and nine experiments were performed 24 or 48 hours later. Significant dilatation of the basilar artery was achieved in all cases and it persisted for as long as the infusion of nitroprusside continued. The drug produced a modest degree of systemic hypotension. In six experiments it was possible to avoid hypotension by a simultaneous infusion of dopamine.", "contents": "Reversal of experimental cerebral vasospasm by intravenous nitroprusside therapy. Cerebral vasospasm was induced in dogs by intracisternal injection of blood. After angiographic demonstration of spasm, sodium nitroprusside was infused intravenously and its effect on the diameter of the basilar artery was studied angiographically. Ten experiments were performed within 90 minutes of the induction of spasm and nine experiments were performed 24 or 48 hours later. Significant dilatation of the basilar artery was achieved in all cases and it persisted for as long as the infusion of nitroprusside continued. The drug produced a modest degree of systemic hypotension. In six experiments it was possible to avoid hypotension by a simultaneous infusion of dopamine.", "PMID": 968722}
{"id": "pubmed23n0032_7377", "title": "Giant aneurysm of the middle cerebral artery.", "content": "A case of a giant aneurysm of the middle cerebral artery, (4.5 X 4.5 X 9.5 CM) presenting as a mass lesion, which was successfully excised is described. This case is compared to the few previous accounts of giant aneurysms of the middle cerebral artery larger than 3 cm in diameter.", "contents": "Giant aneurysm of the middle cerebral artery. A case of a giant aneurysm of the middle cerebral artery, (4.5 X 4.5 X 9.5 CM) presenting as a mass lesion, which was successfully excised is described. This case is compared to the few previous accounts of giant aneurysms of the middle cerebral artery larger than 3 cm in diameter.", "PMID": 968723}
{"id": "pubmed23n0032_7378", "title": "The value of sequential scanning in the detection of metastatic tumors.", "content": "The usefulness of sequential scanning, three and 24 hours after injection of 197Hg chlormerodrin in the detection of metastatic tumors of the brain has been demonstrated. The 24-hour scan was positive in 29% of the cases in which a metastatic lesion was suspected although the three-hour scan was negative, and the 24-hour scan also revealed additional masses in another 20%. The delayed scan helped to demarcate the actual location of the lesion in 24% of the cases in which the three-hour scan was graded merely suspicious. It is concluded that the addition of a 24-hour scan strongly reinforces the statistical accuracy of the 197Hg brain scan, and thus is of practical clinical importance.", "contents": "The value of sequential scanning in the detection of metastatic tumors. The usefulness of sequential scanning, three and 24 hours after injection of 197Hg chlormerodrin in the detection of metastatic tumors of the brain has been demonstrated. The 24-hour scan was positive in 29% of the cases in which a metastatic lesion was suspected although the three-hour scan was negative, and the 24-hour scan also revealed additional masses in another 20%. The delayed scan helped to demarcate the actual location of the lesion in 24% of the cases in which the three-hour scan was graded merely suspicious. It is concluded that the addition of a 24-hour scan strongly reinforces the statistical accuracy of the 197Hg brain scan, and thus is of practical clinical importance.", "PMID": 968724}
{"id": "pubmed23n0032_7379", "title": "Treatment of experimental spinal cord injury in ferrets.", "content": "A variety of treatment modalities upon a compressive model of spinal cord injury in ferrets was tested. Several of the treatments seemed to increase the degree of anatomical and/or functional sparing, but only dexamethasone did so in a statistically significant manner.", "contents": "Treatment of experimental spinal cord injury in ferrets. A variety of treatment modalities upon a compressive model of spinal cord injury in ferrets was tested. Several of the treatments seemed to increase the degree of anatomical and/or functional sparing, but only dexamethasone did so in a statistically significant manner.", "PMID": 968725}
{"id": "pubmed23n0032_7380", "title": "The management of intracranial calcified subdural hematomas.", "content": "Calcified subdural hematomas are associated with signs and symptoms similar to those of chronic, uncalcified subdural hematomas. Those found incidentally or in patients with long-standing nonprogressive neurological deficits should be left alone. If a calcified subdural hematoma is discovered in the evaluation of a patient with an acute or a progressing neurological disorder, an operation should be considered.", "contents": "The management of intracranial calcified subdural hematomas. Calcified subdural hematomas are associated with signs and symptoms similar to those of chronic, uncalcified subdural hematomas. Those found incidentally or in patients with long-standing nonprogressive neurological deficits should be left alone. If a calcified subdural hematoma is discovered in the evaluation of a patient with an acute or a progressing neurological disorder, an operation should be considered.", "PMID": 968726}
{"id": "pubmed23n0032_7381", "title": "\"\"Non-communicating'' syringomyelia: a non-existent entity.", "content": "Encroachment by man's overlarge forebrain is responsible for the anatomic substrate of syringomyelia, i.e. a hindbrain hernia developing in fetal life with persisting hydromyelia. Communication between the syrinx and 4th ventricle is readily disclosed at operation, but because of postmortem shrinkage, almost never at autopsy. Syringomyelia developing in 16 of 864 post-traumatic paraplegics has been classified non-communicating. However, the syrinx, its fluid, and the ultimate clinical picture are the same as in the non-traumatic, and surgical exposure has disclosed the communication. The symptoms develop because the traumatic subarachnoid block exaggerates the causative intracranial fluid pulse waves by eliminating the dampling effect of the yielding dural sac below. A non-traumatic spinal block also may result in syringomyelia.", "contents": "\"\"Non-communicating'' syringomyelia: a non-existent entity. Encroachment by man's overlarge forebrain is responsible for the anatomic substrate of syringomyelia, i.e. a hindbrain hernia developing in fetal life with persisting hydromyelia. Communication between the syrinx and 4th ventricle is readily disclosed at operation, but because of postmortem shrinkage, almost never at autopsy. Syringomyelia developing in 16 of 864 post-traumatic paraplegics has been classified non-communicating. However, the syrinx, its fluid, and the ultimate clinical picture are the same as in the non-traumatic, and surgical exposure has disclosed the communication. The symptoms develop because the traumatic subarachnoid block exaggerates the causative intracranial fluid pulse waves by eliminating the dampling effect of the yielding dural sac below. A non-traumatic spinal block also may result in syringomyelia.", "PMID": 968727}
{"id": "pubmed23n0032_7382", "title": "Ischemic colitis incidence following abdominal aortic reconstruction: a prospective study.", "content": "With few exceptions, the incidence of left colon ischemia following abdominal aortic reconstruction has been reported to be one to 2 percent. All reports of such ischemic events are retrospective analyses of clinically manifest or autopsy. Fifty patients were studied prospectively to determine more accurately the incidence of this complication. Aortic reconstruction was performed in 23 patients for occlusive disease (OD) and in 27 for aneurysm disease (AD). No emergency operations were performed. All patients underwent colonoscopy within 4 days of operation. Three instances of colon ischemia were noted, an incidence of 6 percent (OD 4.3 percent, AD 7.4 percent). Each patient recovered uneventfully. Two patients had diarrhea, but only after colon ischemia was recognized. Arteriographic opacification of the inferior mesenteric artery by the superior mesenteric artery collateral (meandering mesenteric artery) was documented in 35 percent of patients with OD and in 27 percent of patients with AD. Colon ischemia did not develop when this collateral was identified. The inferior mesenteric artery was patent at the aorta in all who developed colon ischema. Although clinically significant colitis following aortic reconstruction is rare, colonoscopy after operation may prove to be valuable for early recognition of ischemic changes before clinical manifestations preclude effective management.", "contents": "Ischemic colitis incidence following abdominal aortic reconstruction: a prospective study. With few exceptions, the incidence of left colon ischemia following abdominal aortic reconstruction has been reported to be one to 2 percent. All reports of such ischemic events are retrospective analyses of clinically manifest or autopsy. Fifty patients were studied prospectively to determine more accurately the incidence of this complication. Aortic reconstruction was performed in 23 patients for occlusive disease (OD) and in 27 for aneurysm disease (AD). No emergency operations were performed. All patients underwent colonoscopy within 4 days of operation. Three instances of colon ischemia were noted, an incidence of 6 percent (OD 4.3 percent, AD 7.4 percent). Each patient recovered uneventfully. Two patients had diarrhea, but only after colon ischemia was recognized. Arteriographic opacification of the inferior mesenteric artery by the superior mesenteric artery collateral (meandering mesenteric artery) was documented in 35 percent of patients with OD and in 27 percent of patients with AD. Colon ischemia did not develop when this collateral was identified. The inferior mesenteric artery was patent at the aorta in all who developed colon ischema. Although clinically significant colitis following aortic reconstruction is rare, colonoscopy after operation may prove to be valuable for early recognition of ischemic changes before clinical manifestations preclude effective management.", "PMID": 968729}
{"id": "pubmed23n0032_7383", "title": "Radiation injuries to the bowel associated with the treatment of carcinoma of the cervix.", "content": "Advances in radiation techniques and increased dosage have improved the cure rate of patients with cancer of the cervix to 65 percent. Associated with this increased dosage (betatron, 5,250 r and intracavitary 137-cesium, 4,000 r at point A) has been a serious complication incidence of 10 percent. Major intestinal complications usually become manifest within an 8 to 24 month period following radiation. Few are associated with tumor and the majority are amenable to surgical correction. Rectosigmoid stenosis is a common and frequently unrecognized complication. The 8 to 12 cm. segment of rectosigmoid, with its rigid wall and narrowed lumen, can be recognized on barium examination. The symptoms are those on incomplete obstruction and deterioration, frequently confused with tumor progression. Thirty-one patients have been treated by resection and low anterior anastomosis with relief of symptoms. Rectosigmoid stenosis progressing to necrosis, perforation, or fistula (an additional 29 patients) is treated best by the Hartmann operation as a first stage. This procedure has been less complicated than either colostomy alone or resection and anastomosis. Fifteen patients with low level rectovaginal fistula or stenosis were treated by defunctioning sigmoid colostomy. A loop transverse colostomy was unsatisfactory. Ileorectovaginal fistulas occurred in an additional six patients. Preoperative investigation should establish the presence or absence of an ileal component in all fistulas. Radiation ileitis is rare as an isolated finding but frequently is associated with severe rectosigmoid damage. Surgical treatment is seldom necessary but, if indicated (ten patients), resection appears to be preferable to bypass.", "contents": "Radiation injuries to the bowel associated with the treatment of carcinoma of the cervix. Advances in radiation techniques and increased dosage have improved the cure rate of patients with cancer of the cervix to 65 percent. Associated with this increased dosage (betatron, 5,250 r and intracavitary 137-cesium, 4,000 r at point A) has been a serious complication incidence of 10 percent. Major intestinal complications usually become manifest within an 8 to 24 month period following radiation. Few are associated with tumor and the majority are amenable to surgical correction. Rectosigmoid stenosis is a common and frequently unrecognized complication. The 8 to 12 cm. segment of rectosigmoid, with its rigid wall and narrowed lumen, can be recognized on barium examination. The symptoms are those on incomplete obstruction and deterioration, frequently confused with tumor progression. Thirty-one patients have been treated by resection and low anterior anastomosis with relief of symptoms. Rectosigmoid stenosis progressing to necrosis, perforation, or fistula (an additional 29 patients) is treated best by the Hartmann operation as a first stage. This procedure has been less complicated than either colostomy alone or resection and anastomosis. Fifteen patients with low level rectovaginal fistula or stenosis were treated by defunctioning sigmoid colostomy. A loop transverse colostomy was unsatisfactory. Ileorectovaginal fistulas occurred in an additional six patients. Preoperative investigation should establish the presence or absence of an ileal component in all fistulas. Radiation ileitis is rare as an isolated finding but frequently is associated with severe rectosigmoid damage. Surgical treatment is seldom necessary but, if indicated (ten patients), resection appears to be preferable to bypass.", "PMID": 968730}
{"id": "pubmed23n0032_7384", "title": "Intraoperative autotransfusion: an underutilized technique.", "content": "Although interest in intraoperative autotransfusion increased when commercial equipment became available, this technique still is utilized rarely in most hospitals. Our experience began with sporadic use in 1972 and has evolved to regular use at least ten times a month. The machine is operated by a technician, and we heparinize the autotransfusion system (ATS) reservoir. Our series includes 47 patients who had elective vascular operations and 141 who had emergency operations, usually for trauma. In the latter group, seven patients who were autotransfused with blood contaminated by intestinal contents survived near fatal injuries and did not develop complications attributable to the procedure. Morbidity and mortality rates in both groups did not appear to be increased as a result of intraoperative autotransfusion. Controversy over methods of anticoagulation and apprehension about effect on blood are not valid reasons for underutilization of this technique. Although significant administrative commitments are required to implement its use and to treat the coagulopathy that accompanies massive reinfusions, they are justified by the value of intraoperative autotransfusion in most cases in which two or more units of blood would be required ordinarily.", "contents": "Intraoperative autotransfusion: an underutilized technique. Although interest in intraoperative autotransfusion increased when commercial equipment became available, this technique still is utilized rarely in most hospitals. Our experience began with sporadic use in 1972 and has evolved to regular use at least ten times a month. The machine is operated by a technician, and we heparinize the autotransfusion system (ATS) reservoir. Our series includes 47 patients who had elective vascular operations and 141 who had emergency operations, usually for trauma. In the latter group, seven patients who were autotransfused with blood contaminated by intestinal contents survived near fatal injuries and did not develop complications attributable to the procedure. Morbidity and mortality rates in both groups did not appear to be increased as a result of intraoperative autotransfusion. Controversy over methods of anticoagulation and apprehension about effect on blood are not valid reasons for underutilization of this technique. Although significant administrative commitments are required to implement its use and to treat the coagulopathy that accompanies massive reinfusions, they are justified by the value of intraoperative autotransfusion in most cases in which two or more units of blood would be required ordinarily.", "PMID": 968731}
{"id": "pubmed23n0032_7385", "title": "Gallstone pancreatitis: pathophysiology.", "content": "The stools of 45 patients with proven gallstones pancreatitis were screened for gallstones. An equal number of peripheral with gallstones but without pancreatitis served as the control group. Gallstones were found in the stools of 38 of the 45 patients (84 percent) with gallstone pancreatitis and in only five (11 percent) patients of the control group. The patients with gallstone pancreatitis experienced a relief of symptoms and a decrease in the levels of serum amylase and bilirubin prior to rectal passage of the stones. Operative cholangiography revealed reflux of contrast material into the pancreatic duct of 67 percent of the patients with gallstone pancreatitis and in only 18 percent of the controls. Of the 38 patients that passed stones, 30 cholangiograms (79 percent) demonstrated a functioning common channel. it would appear that a functioning common channel is necessary for reflux and in addition favors stone passage. This study suggests that the pathophysiology of gallstone pancreatitis relates to the temporary impaction of migrating stones at the ampulla of Vater.", "contents": "Gallstone pancreatitis: pathophysiology. The stools of 45 patients with proven gallstones pancreatitis were screened for gallstones. An equal number of peripheral with gallstones but without pancreatitis served as the control group. Gallstones were found in the stools of 38 of the 45 patients (84 percent) with gallstone pancreatitis and in only five (11 percent) patients of the control group. The patients with gallstone pancreatitis experienced a relief of symptoms and a decrease in the levels of serum amylase and bilirubin prior to rectal passage of the stones. Operative cholangiography revealed reflux of contrast material into the pancreatic duct of 67 percent of the patients with gallstone pancreatitis and in only 18 percent of the controls. Of the 38 patients that passed stones, 30 cholangiograms (79 percent) demonstrated a functioning common channel. it would appear that a functioning common channel is necessary for reflux and in addition favors stone passage. This study suggests that the pathophysiology of gallstone pancreatitis relates to the temporary impaction of migrating stones at the ampulla of Vater.", "PMID": 968732}
{"id": "pubmed23n0032_7386", "title": "Jejunoileal bypass for morbid obesity: early results and body composition changes in forty-five patients.", "content": "Forty-five patients who underwent a 14 by 4 inch jejunoileal bypass for morbid obesity were studied before the operation and at periodic intervals after operation to determine the complications and changes in body composition resulting from this procedure. Body composition studies were determined using 3H2O and 42K. Rapid weight loss occurred in the first 3 months, with a mean loss of 30 percent of excess weight. This weight loss was accompanied by a decrease in exchangeable potassium (Ke) and total body water (TBW) during this interval by 14 and 10 percent, respectively. Although most patients continued to lose excess weight Ke and TBW stabilized at the end of the first year and returned to preoperative values in six patients at the end of 24 months. Analysis of the ratios of body cell mass and total body water to weight shows an improvement of body composition 12 months after operation. Body composition studies permit a quantitative assessment of the nutritional status in patients undergoing jejunoileal bypass. In spite of significant complications (23 percent), surgery for morbid obesity appears to satisfy the objective of allowing desirable loss of fat with relative sparing of muscle and other supporting tissues.", "contents": "Jejunoileal bypass for morbid obesity: early results and body composition changes in forty-five patients. Forty-five patients who underwent a 14 by 4 inch jejunoileal bypass for morbid obesity were studied before the operation and at periodic intervals after operation to determine the complications and changes in body composition resulting from this procedure. Body composition studies were determined using 3H2O and 42K. Rapid weight loss occurred in the first 3 months, with a mean loss of 30 percent of excess weight. This weight loss was accompanied by a decrease in exchangeable potassium (Ke) and total body water (TBW) during this interval by 14 and 10 percent, respectively. Although most patients continued to lose excess weight Ke and TBW stabilized at the end of the first year and returned to preoperative values in six patients at the end of 24 months. Analysis of the ratios of body cell mass and total body water to weight shows an improvement of body composition 12 months after operation. Body composition studies permit a quantitative assessment of the nutritional status in patients undergoing jejunoileal bypass. In spite of significant complications (23 percent), surgery for morbid obesity appears to satisfy the objective of allowing desirable loss of fat with relative sparing of muscle and other supporting tissues.", "PMID": 968733}
{"id": "pubmed23n0032_7387", "title": "Gastric bypass for morbid obesity: results and complications.", "content": "Seventy-five patients underwent a 90 percent distal gastric bypass for morbid obesity. The average weight was 121.4 kilogram, height 164 centimeters, and age 31.4 years in these 70 women and five men. There was a total of 20 surgical complications in the 75 patients, with wound infection being the most common. Sixteen chronic complications were noted and consisted of vomiting, diarrhea, reflux esophagitis, dysphagia, and vitamin deficiencies. None of the above complications were life-threatening or required dismantling of the bypass. Of 54 patients followed for 12 months or more after gastric bypass, there was a 24.5 percent average weight decrease at 6 months, and this progressed to 35.8 percent by 12 months. Fifty-two patients undergoing small bowel bypass previously at the same institution had a 25.4 percent weight reduction at 12 months. Of 54 patients, 83 percent followed for one or more years after gastric bypass have had an excellent or good clinical result, whereas only 42 percent of the 52 patients undergoing small bowel bypass have had an excellent or good clinical result with the same criteria. It is concluded that the Mason 90 percent distal gastric bypass is a suitable form of surgical treatment for the morbidity obese patient who cannot lose weight by dietary measures.", "contents": "Gastric bypass for morbid obesity: results and complications. Seventy-five patients underwent a 90 percent distal gastric bypass for morbid obesity. The average weight was 121.4 kilogram, height 164 centimeters, and age 31.4 years in these 70 women and five men. There was a total of 20 surgical complications in the 75 patients, with wound infection being the most common. Sixteen chronic complications were noted and consisted of vomiting, diarrhea, reflux esophagitis, dysphagia, and vitamin deficiencies. None of the above complications were life-threatening or required dismantling of the bypass. Of 54 patients followed for 12 months or more after gastric bypass, there was a 24.5 percent average weight decrease at 6 months, and this progressed to 35.8 percent by 12 months. Fifty-two patients undergoing small bowel bypass previously at the same institution had a 25.4 percent weight reduction at 12 months. Of 54 patients, 83 percent followed for one or more years after gastric bypass have had an excellent or good clinical result, whereas only 42 percent of the 52 patients undergoing small bowel bypass have had an excellent or good clinical result with the same criteria. It is concluded that the Mason 90 percent distal gastric bypass is a suitable form of surgical treatment for the morbidity obese patient who cannot lose weight by dietary measures.", "PMID": 968734}
{"id": "pubmed23n0032_7388", "title": "Comparative value of four methods of investigating the pancreas.", "content": "In a prospective study for the diagnosis of pancreatic cancer, ultrasonography, radionuclide scanning, selective arteriography, and endoscopic retrograde cholangiopancreatography (ERCP) were compared. Eighty-nine consecutive patients were investigated; 58 underwent laparotomy, and 36 were found to have periampullary cancer; seven had other malignant tumors within the abdomen, and nine had pancreatitis on biopsy. Five had other benign disease, and there was one negative laparotomy. Thirty-one patients who did not have laparotomy have shown no evidence of cancer at one year follow-up. Ultrasonography was found to be more reliable than scan or arteriography in the detection and diagnosis of a mass in the pancreas. ERCP achieved the highest rate of correct definitive diagnosis.", "contents": "Comparative value of four methods of investigating the pancreas. In a prospective study for the diagnosis of pancreatic cancer, ultrasonography, radionuclide scanning, selective arteriography, and endoscopic retrograde cholangiopancreatography (ERCP) were compared. Eighty-nine consecutive patients were investigated; 58 underwent laparotomy, and 36 were found to have periampullary cancer; seven had other malignant tumors within the abdomen, and nine had pancreatitis on biopsy. Five had other benign disease, and there was one negative laparotomy. Thirty-one patients who did not have laparotomy have shown no evidence of cancer at one year follow-up. Ultrasonography was found to be more reliable than scan or arteriography in the detection and diagnosis of a mass in the pancreas. ERCP achieved the highest rate of correct definitive diagnosis.", "PMID": 968735}
{"id": "pubmed23n0032_7389", "title": "Delineation of critical factors in the treatment of pancreatic trauma.", "content": "An analysis of 100 patients sustaining multiple injury and pancreatic trauma was completed. Sixteen patients with penetrating injury died within the first 24 hours, 14 of whom died intraoperatively from major hepatic and/or retroperitoneal venous injury. Eighty-four patients survived long enough to permit evaluation of treatment. There was no statistically significant relationship between mode (p = 0.3) or anatomic area (p = 0.5) of injury and death. However, death was more common in the presence of duct injury (p less than 0.0001). Thirty-nine patients were determined to have duct injury and 45 did not. These two groups were equivalent, with the exception of a higher incidence of concomitant bowel injury (p less than 0.05) in those with duct violation. Combined sump and Penrose drainage was found to be adequate treatment of both proximal and distal nonductal injury with no significant difference in mortality or morbidity rates (p = 0.5). Resection of distal ductal injuries as opposed to drainage alone resulted in significantly lower morbidity and mortality rates (p less than 0.05), comparable to those of drained nonductal injuries. No conclusions could be made relevant to proximal duct injuries, except that drainage alone is inadequate. Seventeen (20 percent) of the 84 patients evaluated died. Pancreatic related mortality rate was 17 percent (14 patients). Two of 23 patients with blunt injury (9 percent) and 12 of 61 patients with penetrating injury (20 percent died). Gram-negative sepsis (82 percent) was the most common cause of death (p less than 0.01), and sepsis was correlated with the presence of pancreatic duct (p less than 0.0001) and bowel (p less than 0.001) injury.", "contents": "Delineation of critical factors in the treatment of pancreatic trauma. An analysis of 100 patients sustaining multiple injury and pancreatic trauma was completed. Sixteen patients with penetrating injury died within the first 24 hours, 14 of whom died intraoperatively from major hepatic and/or retroperitoneal venous injury. Eighty-four patients survived long enough to permit evaluation of treatment. There was no statistically significant relationship between mode (p = 0.3) or anatomic area (p = 0.5) of injury and death. However, death was more common in the presence of duct injury (p less than 0.0001). Thirty-nine patients were determined to have duct injury and 45 did not. These two groups were equivalent, with the exception of a higher incidence of concomitant bowel injury (p less than 0.05) in those with duct violation. Combined sump and Penrose drainage was found to be adequate treatment of both proximal and distal nonductal injury with no significant difference in mortality or morbidity rates (p = 0.5). Resection of distal ductal injuries as opposed to drainage alone resulted in significantly lower morbidity and mortality rates (p less than 0.05), comparable to those of drained nonductal injuries. No conclusions could be made relevant to proximal duct injuries, except that drainage alone is inadequate. Seventeen (20 percent) of the 84 patients evaluated died. Pancreatic related mortality rate was 17 percent (14 patients). Two of 23 patients with blunt injury (9 percent) and 12 of 61 patients with penetrating injury (20 percent died). Gram-negative sepsis (82 percent) was the most common cause of death (p less than 0.01), and sepsis was correlated with the presence of pancreatic duct (p less than 0.0001) and bowel (p less than 0.001) injury.", "PMID": 968736}
{"id": "pubmed23n0032_7390", "title": "Massive gastrointestinal hemorrhage: a twenty-five year experience with vagotomy and drainage.", "content": "Old age and massive hemorrhage long have been recognized as ominous prognostic signs in the management of patients with peptic ulcer disease. The authors present the results of their long-term evaluation of 73 such patients. The group was unselected, critically ill, and treated surgically for the arrest of profuse hemorrhage. Accurate follow-up was achieved for 100 percent of the group; this follow-up spanned periods extending to 25 years. In comparison to published reports of similar high risk groups treated with resectional therapy, the vagotomy and pyloroplasty is shown to be comparable in long-term results and vastly superior in terms of operative mortality rate. The mortality rate for the advocated procedure was 8 percent, with a proven recurrence rate of 3.1 percent.", "contents": "Massive gastrointestinal hemorrhage: a twenty-five year experience with vagotomy and drainage. Old age and massive hemorrhage long have been recognized as ominous prognostic signs in the management of patients with peptic ulcer disease. The authors present the results of their long-term evaluation of 73 such patients. The group was unselected, critically ill, and treated surgically for the arrest of profuse hemorrhage. Accurate follow-up was achieved for 100 percent of the group; this follow-up spanned periods extending to 25 years. In comparison to published reports of similar high risk groups treated with resectional therapy, the vagotomy and pyloroplasty is shown to be comparable in long-term results and vastly superior in terms of operative mortality rate. The mortality rate for the advocated procedure was 8 percent, with a proven recurrence rate of 3.1 percent.", "PMID": 968737}
{"id": "pubmed23n0032_7391", "title": "A comparison of wedge and segmental resection of the lung.", "content": "In the past few years there has been increasing use of limited resection for pulmonary carcinoma, especially in patients with restricted cardiorespiratory function. Because there is frequently a choice as to the type of limited resection, it was considered worth while to review the safety and efficiency of the two principal types. In total, 212 wedge resections and 281 segmental resections are reported. Despite certain theoretical advantages to segmentectomy, wedge resection carried a lower complication rate. Seventy-one per cent of wedge resections were free of complications compared to 54% of segmental resections. Minor complications were defined as apical air space and apical haematoma. The incidence of minor complications was similar for each group, 22% for wedge resections and 27% for segmentectomies. However, there was a significantly higher major complication rate in the segmental resection group (19%) compared to the wedge group (7%). This is understandable, considering the amount of raw lung surface exposed after segmental resection. It appears that where it is surgically feasible, wedge resection should be practised.", "contents": "A comparison of wedge and segmental resection of the lung. In the past few years there has been increasing use of limited resection for pulmonary carcinoma, especially in patients with restricted cardiorespiratory function. Because there is frequently a choice as to the type of limited resection, it was considered worth while to review the safety and efficiency of the two principal types. In total, 212 wedge resections and 281 segmental resections are reported. Despite certain theoretical advantages to segmentectomy, wedge resection carried a lower complication rate. Seventy-one per cent of wedge resections were free of complications compared to 54% of segmental resections. Minor complications were defined as apical air space and apical haematoma. The incidence of minor complications was similar for each group, 22% for wedge resections and 27% for segmentectomies. However, there was a significantly higher major complication rate in the segmental resection group (19%) compared to the wedge group (7%). This is understandable, considering the amount of raw lung surface exposed after segmental resection. It appears that where it is surgically feasible, wedge resection should be practised.", "PMID": 968791}
{"id": "pubmed23n0032_7392", "title": "Spontaneous pneumothorax: the sharp rib syndrome.", "content": "Of 49 patients undergoing operation for benign spontaneous pneumothorax, 28 (57%) were found to have a sharp first or second rib. In a series of 100 patients undergoing thoracotomy for other conditions, only eight (8%) were found to have a sharp rib. The association between sharp ribs, apical scars and bullae, and spontaneous pneumothorax is discussed.", "contents": "Spontaneous pneumothorax: the sharp rib syndrome. Of 49 patients undergoing operation for benign spontaneous pneumothorax, 28 (57%) were found to have a sharp first or second rib. In a series of 100 patients undergoing thoracotomy for other conditions, only eight (8%) were found to have a sharp rib. The association between sharp ribs, apical scars and bullae, and spontaneous pneumothorax is discussed.", "PMID": 968792}
{"id": "pubmed23n0032_7393", "title": "Infective endocarditis: a survey of cases in the South-East region of Scotland, 1969-72.", "content": "The incidence and characteristics of infective endocarditis were studied in a defined community over a four-year period. Seventy-eight cases were found, giving an incidence of 16 cases permillion per year. The commonest presenting features were those of infection; 53% had cardiac failure and 37% evidence of emboli when first seen. Twenty-three cases occurred on rheumatic heart valves, 13 on valvular prostheses, and 19 in previously normal hearts. Streptococcus viridans was the commonest organism, but there was a relatively high incidence of staphylococcal infection. Only four cases were preceded by dental manipulation, and no source for the infection was found in 46 patients. The mortality rate was 46%, cardiac failure and embolic phenomena accounting for 65% of deaths. It is unlikely that earlier diagnosis or cardiac surgery would have reduced the mortality appreciably.", "contents": "Infective endocarditis: a survey of cases in the South-East region of Scotland, 1969-72. The incidence and characteristics of infective endocarditis were studied in a defined community over a four-year period. Seventy-eight cases were found, giving an incidence of 16 cases permillion per year. The commonest presenting features were those of infection; 53% had cardiac failure and 37% evidence of emboli when first seen. Twenty-three cases occurred on rheumatic heart valves, 13 on valvular prostheses, and 19 in previously normal hearts. Streptococcus viridans was the commonest organism, but there was a relatively high incidence of staphylococcal infection. Only four cases were preceded by dental manipulation, and no source for the infection was found in 46 patients. The mortality rate was 46%, cardiac failure and embolic phenomena accounting for 65% of deaths. It is unlikely that earlier diagnosis or cardiac surgery would have reduced the mortality appreciably.", "PMID": 968793}
{"id": "pubmed23n0032_7394", "title": "Pericardiectomy in children.", "content": "Thirty-five children under 16 years of age were prepared for pericardiectomy on account of constrictive pericarditis; three died in the immediate preparatory period. Pericardiectomy was undertaken in 32; in 28 the cause was tuberculous. The purpose of this report is to draw attention to the high incidence of constrictive pericarditis in African children in Natal.", "contents": "Pericardiectomy in children. Thirty-five children under 16 years of age were prepared for pericardiectomy on account of constrictive pericarditis; three died in the immediate preparatory period. Pericardiectomy was undertaken in 32; in 28 the cause was tuberculous. The purpose of this report is to draw attention to the high incidence of constrictive pericarditis in African children in Natal.", "PMID": 968794}
{"id": "pubmed23n0032_7395", "title": "An evaluation of Potts' aortopulmonary shunt for palliation of cyanotic heart disease.", "content": "The results of 28 Potts' aortopulmonary shunts created for the relief of cyanotic heart disease are reviewed in this study. The shunt gave excellent symptomatic relief, but the incidence of immediate and late complications is high. Regular follow-up of patients is mandatory to detect evidence of increasing pulmonary vascular disease and to under take corrective surgery whenever feasible before its occurrence. Although Potts' anastomosis has been largely replaced by alternative shunt procedures, there may still be a place for its application in selected situations. Only a few problems were encountered at the time of closure of the shunt in 11 patients during corrective surgery using a transpulmonary technique and hypothermia with circulatory arrest.", "contents": "An evaluation of Potts' aortopulmonary shunt for palliation of cyanotic heart disease. The results of 28 Potts' aortopulmonary shunts created for the relief of cyanotic heart disease are reviewed in this study. The shunt gave excellent symptomatic relief, but the incidence of immediate and late complications is high. Regular follow-up of patients is mandatory to detect evidence of increasing pulmonary vascular disease and to under take corrective surgery whenever feasible before its occurrence. Although Potts' anastomosis has been largely replaced by alternative shunt procedures, there may still be a place for its application in selected situations. Only a few problems were encountered at the time of closure of the shunt in 11 patients during corrective surgery using a transpulmonary technique and hypothermia with circulatory arrest.", "PMID": 968795}
{"id": "pubmed23n0032_7396", "title": "Surgical treatment of congenital valvular aortic stenosis.", "content": "Twenty-two patients with congenital valvular aortic stenosis were surgically treated between 1967 and July 1975. Five (23%) were under 1 year of age (group I) and 17 (77%) were between 2 and 24 years (group II). All infants exhibited severe congestive heart failure and electrocardiographi (ECG) evidence of left ventricular hypertrophy (LVH) with strain pattern. In group II, angina was present in three cases, syncope and fatigue in two; the ECG indicated LVH in 10 cases (59%) with strain pattern in five (29%). A bicuspid aortic valve was present in 77% (17/22) of the cases; 32% had other cardiac anomalies. Aortic valvotomy was performed on cardiopulmonary bypass in 20 cases, and with deep hypothermia and circulatory arrest in two. Three infants under 1 month of age with associated anomalies died (hospital mortality 14%). Intraoperative average peak left ventricular-aortic systolic pressure gradient decreased from 86 to 21 mmHg (P less than 0.001). Late clinical (in all cases) and haemodynamic (26%) follow-up showed severe restenosis in two patients of group II; one of them had a second operation, the other one died three and a half years postoperatively. Results assessed on the basis of symptoms, ECG changes, aortic valve function, and/or haemodynamic findings were fair in the two surviving infants. Results in group II were excellent in three, satisfactory in seven, fair in four, and poor in two cases. In infants, aortic valvotomy is a palliative procedure which carries a high risk. In the older age group, early and late results are more gratifying.", "contents": "Surgical treatment of congenital valvular aortic stenosis. Twenty-two patients with congenital valvular aortic stenosis were surgically treated between 1967 and July 1975. Five (23%) were under 1 year of age (group I) and 17 (77%) were between 2 and 24 years (group II). All infants exhibited severe congestive heart failure and electrocardiographi (ECG) evidence of left ventricular hypertrophy (LVH) with strain pattern. In group II, angina was present in three cases, syncope and fatigue in two; the ECG indicated LVH in 10 cases (59%) with strain pattern in five (29%). A bicuspid aortic valve was present in 77% (17/22) of the cases; 32% had other cardiac anomalies. Aortic valvotomy was performed on cardiopulmonary bypass in 20 cases, and with deep hypothermia and circulatory arrest in two. Three infants under 1 month of age with associated anomalies died (hospital mortality 14%). Intraoperative average peak left ventricular-aortic systolic pressure gradient decreased from 86 to 21 mmHg (P less than 0.001). Late clinical (in all cases) and haemodynamic (26%) follow-up showed severe restenosis in two patients of group II; one of them had a second operation, the other one died three and a half years postoperatively. Results assessed on the basis of symptoms, ECG changes, aortic valve function, and/or haemodynamic findings were fair in the two surviving infants. Results in group II were excellent in three, satisfactory in seven, fair in four, and poor in two cases. In infants, aortic valvotomy is a palliative procedure which carries a high risk. In the older age group, early and late results are more gratifying.", "PMID": 968796}
{"id": "pubmed23n0032_7397", "title": "Cellular aggregation and destruction during blood circulation and oxygenation.", "content": "Platelet and leucocyte aggregation and severe haemolysis may occur in blood during the preparation of an extracorporeal circuit for open-heart surgery. Experiments with dog blood showed that both processes result from bubble oxygenation but not from circulation of diluted blood and that they appear to be inhibited by the presence of acid citrate dextrose and heparin, or citrate phosphate dextrose and heparin, but not by heparin alone.", "contents": "Cellular aggregation and destruction during blood circulation and oxygenation. Platelet and leucocyte aggregation and severe haemolysis may occur in blood during the preparation of an extracorporeal circuit for open-heart surgery. Experiments with dog blood showed that both processes result from bubble oxygenation but not from circulation of diluted blood and that they appear to be inhibited by the presence of acid citrate dextrose and heparin, or citrate phosphate dextrose and heparin, but not by heparin alone.", "PMID": 968797}
{"id": "pubmed23n0032_7398", "title": "Effects of alternate-day prednisone therapy on respiratory function in myasthenia gravis.", "content": "To determine the effects of alternate-day prednisone therapy on respiratory function in myasthenia gravis, eight patients were evaluated during \"days on\" and \"days off\" prednisone. The patients were treated with long-term (up to three years), high-single-dose, alternate-day oral prednisone. After patients had been controlled with alternate-day prednisone they had no episodes of acute respiratory insufficiency or myasthenic crises. Although a small reduction in respiratory function during the day off prednisone was seen in some patients, the change was not statistically significant for the group and was probably physiologically unimportant in most patients. The data indicate that in myasthenic patients who have no underlying lung disease respiratory function is not significantly compromised by administering the prednisone on alternate days.", "contents": "Effects of alternate-day prednisone therapy on respiratory function in myasthenia gravis. To determine the effects of alternate-day prednisone therapy on respiratory function in myasthenia gravis, eight patients were evaluated during \"days on\" and \"days off\" prednisone. The patients were treated with long-term (up to three years), high-single-dose, alternate-day oral prednisone. After patients had been controlled with alternate-day prednisone they had no episodes of acute respiratory insufficiency or myasthenic crises. Although a small reduction in respiratory function during the day off prednisone was seen in some patients, the change was not statistically significant for the group and was probably physiologically unimportant in most patients. The data indicate that in myasthenic patients who have no underlying lung disease respiratory function is not significantly compromised by administering the prednisone on alternate days.", "PMID": 968798}
{"id": "pubmed23n0032_7399", "title": "Complement components and IgE in patients with asthma and aspirin idiosyncrasy.", "content": "Levels of circulating IgE, total haemolytic complement, and components C4 and C3 were measured in 16 asthmatics with aspirin idiosyncrasy and in control subjects. IgE levels were mostly within normal limits. No differences were found between the complement profiles-in particular the C4 levels-in the two groups. As low levels of C4 have been found in patients with intrinsic asthma, these results would suggest a fundamental difference between asthmatics with aspirin idiosyncrasy and others with intrinsic asthma.", "contents": "Complement components and IgE in patients with asthma and aspirin idiosyncrasy. Levels of circulating IgE, total haemolytic complement, and components C4 and C3 were measured in 16 asthmatics with aspirin idiosyncrasy and in control subjects. IgE levels were mostly within normal limits. No differences were found between the complement profiles-in particular the C4 levels-in the two groups. As low levels of C4 have been found in patients with intrinsic asthma, these results would suggest a fundamental difference between asthmatics with aspirin idiosyncrasy and others with intrinsic asthma.", "PMID": 968799}
{"id": "pubmed23n0032_7400", "title": "Regional lung function in ankylosing spondylitis.", "content": "Xenon-133 was used to study regional pulmonary function in nine patients with chest cage rigidity due to ankylosing spondylitis. In comparison with normal subjects, the patients showed an overall diminution in lung volume and the proportion of inhaled xenon reaching the lung apices was reduced but the distribution of injected xenon was normal. The possible relationship between these findings and apical lung disease in ankylosing spondylitis is mentioned.", "contents": "Regional lung function in ankylosing spondylitis. Xenon-133 was used to study regional pulmonary function in nine patients with chest cage rigidity due to ankylosing spondylitis. In comparison with normal subjects, the patients showed an overall diminution in lung volume and the proportion of inhaled xenon reaching the lung apices was reduced but the distribution of injected xenon was normal. The possible relationship between these findings and apical lung disease in ankylosing spondylitis is mentioned.", "PMID": 968800}
{"id": "pubmed23n0032_7401", "title": "Regional lung function in unilateral diaphragmatic paralysis.", "content": "Radioactive xenon-133 was used to study the regional lung function of five patients with unilateral diaphragmatic paralysis unassociated with intrathoracic disease. All patients showed a reduction in total lung capacity to which the affected side contributed an average of 37%. There was a decrease in the amount of inhaled xenon and a lesser decrease in the amount of injected xenon reaching the lung base on the paralysed side.The distribution in the opposite lung did not differ significantly from that found in normal subjects although the proportion of inhaled xenon reaching the lung base was rather less than in the normal group. The washout of injected xenon was normal except for slight impairment at the lung base on the paralysed side in one patient and at both bases in another.", "contents": "Regional lung function in unilateral diaphragmatic paralysis. Radioactive xenon-133 was used to study the regional lung function of five patients with unilateral diaphragmatic paralysis unassociated with intrathoracic disease. All patients showed a reduction in total lung capacity to which the affected side contributed an average of 37%. There was a decrease in the amount of inhaled xenon and a lesser decrease in the amount of injected xenon reaching the lung base on the paralysed side.The distribution in the opposite lung did not differ significantly from that found in normal subjects although the proportion of inhaled xenon reaching the lung base was rather less than in the normal group. The washout of injected xenon was normal except for slight impairment at the lung base on the paralysed side in one patient and at both bases in another.", "PMID": 968801}
{"id": "pubmed23n0032_7402", "title": "Electron microscopy in the cytological examination of metastatic pleural effusions.", "content": "In a previous publication the ultrastructure of pleural effusions in cases of pleural mesothelioma was reported. The same method has now been applied to a study of effusions produced by pleural metastases. The findings are considered sufficiently conclusive to justify the use of electron microscopic cytology in determining the nature and sometimes the origin of such effusions.", "contents": "Electron microscopy in the cytological examination of metastatic pleural effusions. In a previous publication the ultrastructure of pleural effusions in cases of pleural mesothelioma was reported. The same method has now been applied to a study of effusions produced by pleural metastases. The findings are considered sufficiently conclusive to justify the use of electron microscopic cytology in determining the nature and sometimes the origin of such effusions.", "PMID": 968802}
{"id": "pubmed23n0032_7403", "title": "Persistent hypoplasia of the lung after repair of congenital diaphragmatic hernia.", "content": "Quantitative analysis has been used to assess growth in a lung from an infant aged 21/2 months in whom a diaphragmatic hernia was repaired at birth. The lungs had been abnormally small at birth but at 21/2 months were of normal volume. Alveoli had multiplied at the normal rate after birth but had not reached the number normal of age. The number per acinus was normal but the alveoli were increased in size, particularly in the left lung. The airway number, and thus alveolar, acinar, and arterial number, were all reduced in both lungs, the ipsilateral being most affected. The pulmonary blood vessels in both lungs showed an increased muscularity that did not correlate with lung volume or alveolar number, a feature that may have been present at birth. The degrees of hypoplasia in the two lungs were different at birth and this difference had been maintained. The effect of the disturbance to lung growth on the functioning of the lung is discussed.", "contents": "Persistent hypoplasia of the lung after repair of congenital diaphragmatic hernia. Quantitative analysis has been used to assess growth in a lung from an infant aged 21/2 months in whom a diaphragmatic hernia was repaired at birth. The lungs had been abnormally small at birth but at 21/2 months were of normal volume. Alveoli had multiplied at the normal rate after birth but had not reached the number normal of age. The number per acinus was normal but the alveoli were increased in size, particularly in the left lung. The airway number, and thus alveolar, acinar, and arterial number, were all reduced in both lungs, the ipsilateral being most affected. The pulmonary blood vessels in both lungs showed an increased muscularity that did not correlate with lung volume or alveolar number, a feature that may have been present at birth. The degrees of hypoplasia in the two lungs were different at birth and this difference had been maintained. The effect of the disturbance to lung growth on the functioning of the lung is discussed.", "PMID": 968803}
{"id": "pubmed23n0032_7404", "title": "Acute thoracic inlet obstruction in achalasia of the oesophagus.", "content": "A case of acute thoracic inlet obstruction presenting as a rare complication of achalasia is described. The probable mechanism, diagnosis, and management are discussed.", "contents": "Acute thoracic inlet obstruction in achalasia of the oesophagus. A case of acute thoracic inlet obstruction presenting as a rare complication of achalasia is described. The probable mechanism, diagnosis, and management are discussed.", "PMID": 968804}
{"id": "pubmed23n0032_7405", "title": "Spontaneous pneumopericardium.", "content": "Spontaneous pneumopericardium is a relatively rare event, although cases have been recorded over the past 130 years. Many were associated with malignancy, trauma, infection or as a complication of recent surgery. Attempts at surgical resolution have been infrequent and survival extremely rare. We describe a patient in whom pneumopericardium developed spontaneously and insidiously, probably being present for some weeks before hospital investigation. Surgical exploration revealed the cause to be a benign gastric ulcer without an hiatus hernia or other diaphragmatic defect. Repair was attempted but the patient died in the early postoperative period. From an extensive review of the literature it is clear that spontaneous perforation of a gastric ulcer into the pericardium must be less rare than some authors have suggested.", "contents": "Spontaneous pneumopericardium. Spontaneous pneumopericardium is a relatively rare event, although cases have been recorded over the past 130 years. Many were associated with malignancy, trauma, infection or as a complication of recent surgery. Attempts at surgical resolution have been infrequent and survival extremely rare. We describe a patient in whom pneumopericardium developed spontaneously and insidiously, probably being present for some weeks before hospital investigation. Surgical exploration revealed the cause to be a benign gastric ulcer without an hiatus hernia or other diaphragmatic defect. Repair was attempted but the patient died in the early postoperative period. From an extensive review of the literature it is clear that spontaneous perforation of a gastric ulcer into the pericardium must be less rare than some authors have suggested.", "PMID": 968805}
{"id": "pubmed23n0032_7406", "title": "Successful surgical repair of congenital total cleft sternum with partial ectopia cordis.", "content": "Total bifid sternum with or without ectopia cordis is a rare congenital anomaly which is treated successfully in a limited number of cases. Several methods of correction have been described using tissue grafts (cartilage, bone), inert artificial prostheses (acrylic plaques, marlex mesh), and techniques to approximate the sternal bands by relaxation of costal cartilages. In our case the method described by Verska was used to approximate the bifid sternal bands. The successful result in our patient proves the efficiency of this technique.", "contents": "Successful surgical repair of congenital total cleft sternum with partial ectopia cordis. Total bifid sternum with or without ectopia cordis is a rare congenital anomaly which is treated successfully in a limited number of cases. Several methods of correction have been described using tissue grafts (cartilage, bone), inert artificial prostheses (acrylic plaques, marlex mesh), and techniques to approximate the sternal bands by relaxation of costal cartilages. In our case the method described by Verska was used to approximate the bifid sternal bands. The successful result in our patient proves the efficiency of this technique.", "PMID": 968806}
{"id": "pubmed23n0032_7407", "title": "Constrictive epicarditis following Coxsackie virus infection.", "content": "A case is presented of the rare condition of constrictive epicarditis, which was treated successfully by removal of the epicardium. This appears to be the first account of constrictive epicarditis following proven Coxsackie viral infection.", "contents": "Constrictive epicarditis following Coxsackie virus infection. A case is presented of the rare condition of constrictive epicarditis, which was treated successfully by removal of the epicardium. This appears to be the first account of constrictive epicarditis following proven Coxsackie viral infection.", "PMID": 968807}
{"id": "pubmed23n0032_7408", "title": "[Shortening of the upper jaw (brachygnathia superior) as a clinical feature of atrophic rhinitis in approximately eight-week-old piglets (author's transl)].", "content": "Determining the degree of shortening of the upper jaw is described as an effective and simple method by which piglets affected with severe atrophic rhinitis may be identified as early as the eighth week of life. A positive high correlation (r2 = 0.61) between shortening of the upper jaw and atrophy of the ventral conchae was found to be present in pigs at this age. Clinical and pathological studies of 2,000 heads of swine of various ages revealed changes of the conchae and nasal bones as well as shortening of the upper jaw in animals affected with atrophic rhinitis. Over 99 per cent of the affected animals showed changes of the conchae at an age of eight weeks. In 95 per cent of the cases, the nasal bones were also involved. In 92 per cent of the animals showing lesions of the conchae, which were pigs of two herds, the shortening of the upper jaw was given a grading of one or higher. In view of these findings, the piglets were selected for severe shorting of the upper jaw and deviation of the axis of the head. The losses caused by atrophic rhinitis were reduced to nil on these fattening farms.", "contents": "[Shortening of the upper jaw (brachygnathia superior) as a clinical feature of atrophic rhinitis in approximately eight-week-old piglets (author's transl)]. Determining the degree of shortening of the upper jaw is described as an effective and simple method by which piglets affected with severe atrophic rhinitis may be identified as early as the eighth week of life. A positive high correlation (r2 = 0.61) between shortening of the upper jaw and atrophy of the ventral conchae was found to be present in pigs at this age. Clinical and pathological studies of 2,000 heads of swine of various ages revealed changes of the conchae and nasal bones as well as shortening of the upper jaw in animals affected with atrophic rhinitis. Over 99 per cent of the affected animals showed changes of the conchae at an age of eight weeks. In 95 per cent of the cases, the nasal bones were also involved. In 92 per cent of the animals showing lesions of the conchae, which were pigs of two herds, the shortening of the upper jaw was given a grading of one or higher. In view of these findings, the piglets were selected for severe shorting of the upper jaw and deviation of the axis of the head. The losses caused by atrophic rhinitis were reduced to nil on these fattening farms.", "PMID": 968891}
{"id": "pubmed23n0032_7409", "title": "[Role of bovine respiratory syncytial virus in the aetiology of viruspneumonia in calves and yearlings (\"pinkengriep\") (author's transl)].", "content": "The clinical picture of \"pinkengriep\", an enzootic form of bronchopneumonia in young cattle, is described. In addition to cough, conjunctivitis and a soporous state, accelerated respiration initially is an outstanding clinical symptom. In some cases, symptoms of fog fever appear during the second stage of the disease. In the autumn of 1973 and that of 1974, a total number of 292 animals with \"pinkengriep\" were serologically examined for known respiratory virus infections and Chlamydia. Complement fixation tests showed that there was significant increase in antibodies to respiratory syncytial virus in 76 per cent of the animals studied. In the case of para-influenza virus 3, virus diarrhoea virus, the adenoviruses of the antigen groups 1, 2 and 3, those of the antigen groups 4 to 10 inclusive and Chlamydia, these proportions were 48, 13, 12, 11 en 10 per cent respectively. On the other hand, symptoms of infection with infectious bovine rhinotracheitis virus were absent in the herds studied. These findings suggest a possible role of bovine respiratory syncytial virus in \"pinkengriep\".", "contents": "[Role of bovine respiratory syncytial virus in the aetiology of viruspneumonia in calves and yearlings (\"pinkengriep\") (author's transl)]. The clinical picture of \"pinkengriep\", an enzootic form of bronchopneumonia in young cattle, is described. In addition to cough, conjunctivitis and a soporous state, accelerated respiration initially is an outstanding clinical symptom. In some cases, symptoms of fog fever appear during the second stage of the disease. In the autumn of 1973 and that of 1974, a total number of 292 animals with \"pinkengriep\" were serologically examined for known respiratory virus infections and Chlamydia. Complement fixation tests showed that there was significant increase in antibodies to respiratory syncytial virus in 76 per cent of the animals studied. In the case of para-influenza virus 3, virus diarrhoea virus, the adenoviruses of the antigen groups 1, 2 and 3, those of the antigen groups 4 to 10 inclusive and Chlamydia, these proportions were 48, 13, 12, 11 en 10 per cent respectively. On the other hand, symptoms of infection with infectious bovine rhinotracheitis virus were absent in the herds studied. These findings suggest a possible role of bovine respiratory syncytial virus in \"pinkengriep\".", "PMID": 968892}
{"id": "pubmed23n0032_7410", "title": "[Co-operation between veterinarian and farm adviser, still an undeveloped field? (AUTHOR'S TRANSL)].", "content": "In actual practice it does not often happen that the veterinarian gets into touch with the farm adviser, or vice versa, to exchange views on a particular farm or on problems in their fields of activity. As a rule, this is due to lack of time and/or insufficient acquaintance with each other. There are two fields in which effective co-operation between the two is possible, viz. the management of problem herds and in herd health practice. Interest is centering increasingly in the latter and it is precisely this field in which the farm adviser may also play an important role. It is claimed that working together at the solution of a (veterinary-agricultural) problem on a particular farm will contribute to a closer acquaintance on both sides. The initiative may be taken either by the veterinarian or by the farm adviser. This will possibly result in a happy collaboration in the future, not only in dealing with problem herds but also in hered health practice on those farms on which this is required (or desired).", "contents": "[Co-operation between veterinarian and farm adviser, still an undeveloped field? (AUTHOR'S TRANSL)]. In actual practice it does not often happen that the veterinarian gets into touch with the farm adviser, or vice versa, to exchange views on a particular farm or on problems in their fields of activity. As a rule, this is due to lack of time and/or insufficient acquaintance with each other. There are two fields in which effective co-operation between the two is possible, viz. the management of problem herds and in herd health practice. Interest is centering increasingly in the latter and it is precisely this field in which the farm adviser may also play an important role. It is claimed that working together at the solution of a (veterinary-agricultural) problem on a particular farm will contribute to a closer acquaintance on both sides. The initiative may be taken either by the veterinarian or by the farm adviser. This will possibly result in a happy collaboration in the future, not only in dealing with problem herds but also in hered health practice on those farms on which this is required (or desired).", "PMID": 968894}
{"id": "pubmed23n0032_7411", "title": "A rapid HLA-D matching method using PHA blasts as responding cells (preliminary data on PHA blasts HLA-D typing).", "content": "Day 3-4 PHA stimulated lymphocytes were found to respond against HLA-D without any period of latency, thus allowing a discriminative MLR reading as early as 24-48 h. The test is therefore characterized by the minute number of responding cells necessary (5 X 10(3)), a one-way reaction without additional treatment of the stimualtion normal lymphocytes (5 x 10(4)), and the rapid obtaining of data. We found the preliminary data testing its concordance with classical HLA-D typing encouraging and worthy of being tested on a larger scale. Using mitogen stimulated recipient cells stored frozen, HLA-D matching with potential donors was found to be feasible within 24-48 h. The mechanism of such a prompt response by mitogen stimulated lymphocytes is unknown but appears to be independent of the nature and dose of a given mitogen.", "contents": "A rapid HLA-D matching method using PHA blasts as responding cells (preliminary data on PHA blasts HLA-D typing). Day 3-4 PHA stimulated lymphocytes were found to respond against HLA-D without any period of latency, thus allowing a discriminative MLR reading as early as 24-48 h. The test is therefore characterized by the minute number of responding cells necessary (5 X 10(3)), a one-way reaction without additional treatment of the stimualtion normal lymphocytes (5 x 10(4)), and the rapid obtaining of data. We found the preliminary data testing its concordance with classical HLA-D typing encouraging and worthy of being tested on a larger scale. Using mitogen stimulated recipient cells stored frozen, HLA-D matching with potential donors was found to be feasible within 24-48 h. The mechanism of such a prompt response by mitogen stimulated lymphocytes is unknown but appears to be independent of the nature and dose of a given mitogen.", "PMID": 968897}
{"id": "pubmed23n0032_7412", "title": "Psoriasis, sacro-iliitis and peripheral arthritis occurring in patients with the same HLA haplotype. A preliminary family report and a hypothetical explanation of the interaction between MHS products.", "content": "The present family investigation has shown that genes within the MHS are mainly responsible for the development of psoriasis or psoriasis-associated arthritic lesions (peripheral arthritis and sacroiliitis). We have hypothetically discussed the possibility that multiple genes, all located within the MHS, act in concert to increase the risk of developing disease to very high levels. This implies that at least two MHS linked genes act in complementary fashion for the development of disease, these genes seem to be able to operate both in the cis and in the trans position. One of these genes would be situated in the chromosomal portion of the MHS which carries the HLA-D locus. Families with a high incidence of disease would show inheritance according to the cis position of genes, when it can be shown that most of the carriers of the specific disease-associated haplotype are affected by disease, whereas in other families, complementarity between two distinct HLA haplotypes with genes acting in the trans position would result in disease.", "contents": "Psoriasis, sacro-iliitis and peripheral arthritis occurring in patients with the same HLA haplotype. A preliminary family report and a hypothetical explanation of the interaction between MHS products. The present family investigation has shown that genes within the MHS are mainly responsible for the development of psoriasis or psoriasis-associated arthritic lesions (peripheral arthritis and sacroiliitis). We have hypothetically discussed the possibility that multiple genes, all located within the MHS, act in concert to increase the risk of developing disease to very high levels. This implies that at least two MHS linked genes act in complementary fashion for the development of disease, these genes seem to be able to operate both in the cis and in the trans position. One of these genes would be situated in the chromosomal portion of the MHS which carries the HLA-D locus. Families with a high incidence of disease would show inheritance according to the cis position of genes, when it can be shown that most of the carriers of the specific disease-associated haplotype are affected by disease, whereas in other families, complementarity between two distinct HLA haplotypes with genes acting in the trans position would result in disease.", "PMID": 968898}
{"id": "pubmed23n0032_7413", "title": "A \"natural\" anti-HLA-A2 antibody reacting with homozygous cells.", "content": "In the serum of a young normal male, never transfused, a cold cytotoxic IgM antibody was found reacting exclusively with A2 homozygous cells on a French panel, as shown by population and family studies. This antibody needs about two to five times more lymphocytes or platelets to be absorbed than an immune anti-A2 of the same titre. The antigen recognized by this antibody seems to be covered by the same molecule as HLA-A2, according to redistribution experiments.", "contents": "A \"natural\" anti-HLA-A2 antibody reacting with homozygous cells. In the serum of a young normal male, never transfused, a cold cytotoxic IgM antibody was found reacting exclusively with A2 homozygous cells on a French panel, as shown by population and family studies. This antibody needs about two to five times more lymphocytes or platelets to be absorbed than an immune anti-A2 of the same titre. The antigen recognized by this antibody seems to be covered by the same molecule as HLA-A2, according to redistribution experiments.", "PMID": 968899}
{"id": "pubmed23n0032_7414", "title": "Rga (Rodgers) and the HLA region: linkage and associations.", "content": "In 19 families with 97 children the segregation of Rga (Rodgers) was found to be compatible with Mendelian inheritance and five backcross and 14 intercross families were found among HLA and Bf typed families. Close linkage (lods + 17.82) without recombination was found between Rg and the HLA region, with a direct count of 96 nonrecombinant meioses for Rg-HLA-B. Rg- was strongly associated with HLA-B8 (29 of 30 haplotypes) and probably associated with Bw40, but did occur on other HLA-B haplotypes. By inference Rg- is negatively associated with Ch- (Chido). The Rg-Ch- haplotype has not been observed. Rga and Cha may or may not be coded for by different sites of the same cistron closely linked to HLA-B:C and cannot as yet be excluded from being parts of B or C.", "contents": "Rga (Rodgers) and the HLA region: linkage and associations. In 19 families with 97 children the segregation of Rga (Rodgers) was found to be compatible with Mendelian inheritance and five backcross and 14 intercross families were found among HLA and Bf typed families. Close linkage (lods + 17.82) without recombination was found between Rg and the HLA region, with a direct count of 96 nonrecombinant meioses for Rg-HLA-B. Rg- was strongly associated with HLA-B8 (29 of 30 haplotypes) and probably associated with Bw40, but did occur on other HLA-B haplotypes. By inference Rg- is negatively associated with Ch- (Chido). The Rg-Ch- haplotype has not been observed. Rga and Cha may or may not be coded for by different sites of the same cistron closely linked to HLA-B:C and cannot as yet be excluded from being parts of B or C.", "PMID": 968900}
{"id": "pubmed23n0032_7415", "title": "Beh\u00e7et disease and the HLA system.", "content": "Seventy-three unrelated patients with Beh\u00e7et disease together with 33 members of seven families with at least two patients per family were tissue typed for 26 antigens of the HLA system. The patients with the complete type of Beh\u00e7et disease were found to have HLA-B5 more significantly than healthy individuals. Family studies suggest that the genes closely linked to HLA locus influence the degree of severity of Beh\u00e7et disease.", "contents": "Beh\u00e7et disease and the HLA system. Seventy-three unrelated patients with Beh\u00e7et disease together with 33 members of seven families with at least two patients per family were tissue typed for 26 antigens of the HLA system. The patients with the complete type of Beh\u00e7et disease were found to have HLA-B5 more significantly than healthy individuals. Family studies suggest that the genes closely linked to HLA locus influence the degree of severity of Beh\u00e7et disease.", "PMID": 968901}
{"id": "pubmed23n0032_7416", "title": "The carcinogenic response in mice to the topical application of propane sultone to the skin.", "content": "The carcinogenic effects of limited and repeated skin applications of propane sultone were investigated in three strains of mice, CF1, C3H and CBah (a hairless strain). Propane sultone was shown to be carcinogenic when given as a single application of a 25% w/v solution in toluene and also following twice weekly application of a 2.5% w/v solution for up to 58 weeks. More limited exposure to 2.5% w/v solutions of propane sultone resulted in a few skin tumours, although the incidences were not statistically significant. Most neoplasms were papillomas or carcinomas, although a small number of mesenchymal tumours of dermal origin also developed. No skin neoplasms were found in any control mice. The skin application of propane sultone was associated with a statistically significant increase in the incidence of systematic neoplasia in CFl and C3H mice. The exposed CFl mice had a higher incidence of neoplasms of lymphoreticular and lung origin, while female C3H mice showed a higher incidence of mammary gland and uterine tumours. In mice exposed to beta-propiolactone as a positive control, neoplasms developed at the site of application but, there was no evidence of increased systemic neoplasis in contrast to the findings with ptopane sultone.", "contents": "The carcinogenic response in mice to the topical application of propane sultone to the skin. The carcinogenic effects of limited and repeated skin applications of propane sultone were investigated in three strains of mice, CF1, C3H and CBah (a hairless strain). Propane sultone was shown to be carcinogenic when given as a single application of a 25% w/v solution in toluene and also following twice weekly application of a 2.5% w/v solution for up to 58 weeks. More limited exposure to 2.5% w/v solutions of propane sultone resulted in a few skin tumours, although the incidences were not statistically significant. Most neoplasms were papillomas or carcinomas, although a small number of mesenchymal tumours of dermal origin also developed. No skin neoplasms were found in any control mice. The skin application of propane sultone was associated with a statistically significant increase in the incidence of systematic neoplasia in CFl and C3H mice. The exposed CFl mice had a higher incidence of neoplasms of lymphoreticular and lung origin, while female C3H mice showed a higher incidence of mammary gland and uterine tumours. In mice exposed to beta-propiolactone as a positive control, neoplasms developed at the site of application but, there was no evidence of increased systemic neoplasis in contrast to the findings with ptopane sultone.", "PMID": 968911}
{"id": "pubmed23n0032_7417", "title": "Subacute toxicity studies with sodium saccharin and two hydrolytic derivatives.", "content": "The subacute toxicity of sodium saccharin and 2 hydrolytic derivatives, o-sulfamoylbenzoic acid (Compound I) and ammonium o-carboxybenzene sulfonate (Compound II) was evaluated by feeding each of the compounds alone at a dietary level of 20 000 ppm to both beagle dogs and albino rats. Additionally, groups of dogs and rats were fed combinations of the 3 materials at levels up to 20 000 ppm (2000 ppm sodium saccharin, 9000 ppm of both Compound I and II). Dogs were maintained on the test diets for 16 weeks, rats for 13 weeks. No signs of a pharmacotoxic response to the test materials were observed. Parameters determined for treated animals, including growth, food consumption, hematologic profiles, clinical blood chemistry studies, urinalyses, organ weight and ratio data, and both gross and microscopic pathologic evaluation, were not significantly different from control values. From these findings, it is suggested that there is little toxicologic hazard associated with ingestion of the 2 hydrolytic derivatives of sodium saccharin.", "contents": "Subacute toxicity studies with sodium saccharin and two hydrolytic derivatives. The subacute toxicity of sodium saccharin and 2 hydrolytic derivatives, o-sulfamoylbenzoic acid (Compound I) and ammonium o-carboxybenzene sulfonate (Compound II) was evaluated by feeding each of the compounds alone at a dietary level of 20 000 ppm to both beagle dogs and albino rats. Additionally, groups of dogs and rats were fed combinations of the 3 materials at levels up to 20 000 ppm (2000 ppm sodium saccharin, 9000 ppm of both Compound I and II). Dogs were maintained on the test diets for 16 weeks, rats for 13 weeks. No signs of a pharmacotoxic response to the test materials were observed. Parameters determined for treated animals, including growth, food consumption, hematologic profiles, clinical blood chemistry studies, urinalyses, organ weight and ratio data, and both gross and microscopic pathologic evaluation, were not significantly different from control values. From these findings, it is suggested that there is little toxicologic hazard associated with ingestion of the 2 hydrolytic derivatives of sodium saccharin.", "PMID": 968910}
{"id": "pubmed23n0032_7418", "title": "Normal and lethal mercury levels in human beings.", "content": "Ethyl mercury in the form of Granosan M was used as a fungicide in dressing grains in Iraq. Disregarding warnings and precautions by the authorities, some villagers used this grain in making their bread. Tissue specimens of poisoned people were analysed for total mercury contents using the flameless atomic absorption spectroscopic technique. The analytical method used is highly sensitive (1 ppb/1% absorbance), and the precision in terms of relative standard deviation (RSD) was about 1.5%. The ranges of mercury content in ppm units in the two cases of poisoning were 8-9 for the kidneys, 6-7 for livers, 3-5 for the cerebella, and about 15 for the blood. The analyses included some other tissues as well. Control values were also present. These were obtained from human beings who died by accident and showed no signs of mercury poisoning.", "contents": "Normal and lethal mercury levels in human beings. Ethyl mercury in the form of Granosan M was used as a fungicide in dressing grains in Iraq. Disregarding warnings and precautions by the authorities, some villagers used this grain in making their bread. Tissue specimens of poisoned people were analysed for total mercury contents using the flameless atomic absorption spectroscopic technique. The analytical method used is highly sensitive (1 ppb/1% absorbance), and the precision in terms of relative standard deviation (RSD) was about 1.5%. The ranges of mercury content in ppm units in the two cases of poisoning were 8-9 for the kidneys, 6-7 for livers, 3-5 for the cerebella, and about 15 for the blood. The analyses included some other tissues as well. Control values were also present. These were obtained from human beings who died by accident and showed no signs of mercury poisoning.", "PMID": 968912}
{"id": "pubmed23n0032_7419", "title": "Inhalation toxicity studies on cigarette smoke. I. A versatile exposure system for inhalation toxicity studies on cigarette smoke.", "content": "A detailed description is given of the design and operation of a new smoking machine built for inhalation toxicity studies with small laboratory animals. Brief details are given of smoke monitoring carried out to determine the extent of loss of particulated matter along the smoke path through the machine.", "contents": "Inhalation toxicity studies on cigarette smoke. I. A versatile exposure system for inhalation toxicity studies on cigarette smoke. A detailed description is given of the design and operation of a new smoking machine built for inhalation toxicity studies with small laboratory animals. Brief details are given of smoke monitoring carried out to determine the extent of loss of particulated matter along the smoke path through the machine.", "PMID": 968913}
{"id": "pubmed23n0032_7420", "title": "Inhalation toxicity studies on cigarette smoke II. Tobacco smoke inhalation dosimetry studies on small laboratory animals.", "content": "A newly developed exposure system has been used to carry out smoke dosimetry studies on rats, mice, hamsters and guinea pigs. In all species, smoke total particulate matter (TPM) deposited in significant amounts in the lower respiratory system (LRS) at dose levels ranging from 0.515 to 1.710 mg TPM/g respiratory tissue. Nasal deposition of smoke particulates did occur in all of the species examined. The significance of these dosimetry data in relation to the conduct of long-term comparative inhalation toxicity studies with tobacco smoke is discussed.", "contents": "Inhalation toxicity studies on cigarette smoke II. Tobacco smoke inhalation dosimetry studies on small laboratory animals. A newly developed exposure system has been used to carry out smoke dosimetry studies on rats, mice, hamsters and guinea pigs. In all species, smoke total particulate matter (TPM) deposited in significant amounts in the lower respiratory system (LRS) at dose levels ranging from 0.515 to 1.710 mg TPM/g respiratory tissue. Nasal deposition of smoke particulates did occur in all of the species examined. The significance of these dosimetry data in relation to the conduct of long-term comparative inhalation toxicity studies with tobacco smoke is discussed.", "PMID": 968915}
{"id": "pubmed23n0032_7421", "title": "[Toxicokinetics of a thioquinoxaline component: the oxythioquinox (author's transl)].", "content": "The absorption and distribution of Morestan 35S in the organism of the rat were studied after administration by stomach tube. It was shown that Morestan, in vivo and in vitro, forms a complex with plasma proteins and particularly with albumins. Most of the plasma Morestan is distributed like this. After intravenous admininstration, the constant of elimination, K13, and the biological half-life determination show that plasma Morestan 35S is eliminated very slowly. The K12 and K21 constants indicate that there is an equilibrium between the plasma compartment and a peripheral compartment. After intragastric administration, the increase of radioactivity in epididymal adipose tissue, indicate that the peripheral compartment should include the adipose tissue.", "contents": "[Toxicokinetics of a thioquinoxaline component: the oxythioquinox (author's transl)]. The absorption and distribution of Morestan 35S in the organism of the rat were studied after administration by stomach tube. It was shown that Morestan, in vivo and in vitro, forms a complex with plasma proteins and particularly with albumins. Most of the plasma Morestan is distributed like this. After intravenous admininstration, the constant of elimination, K13, and the biological half-life determination show that plasma Morestan 35S is eliminated very slowly. The K12 and K21 constants indicate that there is an equilibrium between the plasma compartment and a peripheral compartment. After intragastric administration, the increase of radioactivity in epididymal adipose tissue, indicate that the peripheral compartment should include the adipose tissue.", "PMID": 968914}
{"id": "pubmed23n0032_7422", "title": "Inhalation toxicity studies on cigarette smoke III. Tobacco smoke inhalation dosimetry study on rats.", "content": "Smoke inhalation dosimetry studies have been carried out on rats, using a new exposure system. A range of cigarettes, tobacco types and smoke concentrations was used. Penetration of smoke into the lungs was clearly demonstrated, and loads of total particulate matter (TPM) of up 1 mg were detected in the lower respiratory system of rats. The mass of TPM deposited was affected by the smoke concentration during exposure. Deposition of TPM in the head of the rat was low in relation to total respiratory system deposition. A pattern of predominantly lung deposition was achieved under the conditions used for this series of experiments. This pattern was not affected by changes in smoke dilution level, cigarette or tobacco type.", "contents": "Inhalation toxicity studies on cigarette smoke III. Tobacco smoke inhalation dosimetry study on rats. Smoke inhalation dosimetry studies have been carried out on rats, using a new exposure system. A range of cigarettes, tobacco types and smoke concentrations was used. Penetration of smoke into the lungs was clearly demonstrated, and loads of total particulate matter (TPM) of up 1 mg were detected in the lower respiratory system of rats. The mass of TPM deposited was affected by the smoke concentration during exposure. Deposition of TPM in the head of the rat was low in relation to total respiratory system deposition. A pattern of predominantly lung deposition was achieved under the conditions used for this series of experiments. This pattern was not affected by changes in smoke dilution level, cigarette or tobacco type.", "PMID": 968916}
{"id": "pubmed23n0032_7423", "title": "[Practice of computer use in radiotherapy planning].", "content": "The main problems of radiotherapy planning are discussed with respect to use of computers now being available for a greater number of centers. One of the most essential premises is apart from clear ideas on a modern target volume concept - a sufficiently high speed in producing realistic summarized isodose contours for any radiation therapy arrangement in any individual patient's cross section outline. This problem being solved, those individual summarized isodose figures have to be critically evaluated and therefore the importance of applicable meaningful optimization criteria come into account. The properties of such criteria, which must be quantifiable, generally applicable and really relevant for judgement on quality of a plan, had to be accepted, before automatic optimization procedures could be developed; principles involved are presented. By means of a short series of examples, namely 60Co fixed field combinations with and without use of wedges, combinations of arc therapy for a number of clinical tasks. It has been pointed out, that most experienced estimate by the eye would have been by far insufficient when compared to the automized computer optimization when using such simple criteria as 1. homogeneity of absorbed dose within target volume, 2. numerically limited absorbed dose within areas of risk, 3. as low as possible radiation effects to all \"outside areas\". It seems to be a real danger, that so called isodose libraries, how high their merits might be estimated, may change into an \"isodose bcemetery\", unless we'll be successful for each individual clinical case by use of computers, which are now prepared to supply the best possible variant of the standard plan or the primary radiotherapy idea. Regular use of computers in such a way will furthermore give an incomparable documentation material.", "contents": "[Practice of computer use in radiotherapy planning]. The main problems of radiotherapy planning are discussed with respect to use of computers now being available for a greater number of centers. One of the most essential premises is apart from clear ideas on a modern target volume concept - a sufficiently high speed in producing realistic summarized isodose contours for any radiation therapy arrangement in any individual patient's cross section outline. This problem being solved, those individual summarized isodose figures have to be critically evaluated and therefore the importance of applicable meaningful optimization criteria come into account. The properties of such criteria, which must be quantifiable, generally applicable and really relevant for judgement on quality of a plan, had to be accepted, before automatic optimization procedures could be developed; principles involved are presented. By means of a short series of examples, namely 60Co fixed field combinations with and without use of wedges, combinations of arc therapy for a number of clinical tasks. It has been pointed out, that most experienced estimate by the eye would have been by far insufficient when compared to the automized computer optimization when using such simple criteria as 1. homogeneity of absorbed dose within target volume, 2. numerically limited absorbed dose within areas of risk, 3. as low as possible radiation effects to all \"outside areas\". It seems to be a real danger, that so called isodose libraries, how high their merits might be estimated, may change into an \"isodose bcemetery\", unless we'll be successful for each individual clinical case by use of computers, which are now prepared to supply the best possible variant of the standard plan or the primary radiotherapy idea. Regular use of computers in such a way will furthermore give an incomparable documentation material.", "PMID": 968922}
{"id": "pubmed23n0032_7424", "title": "Inhalation experiments with 14C-labelled cigarette smoke. III. Body size dependent distribution of particulate matter in small rodents during cigarette smoke inhalation.", "content": "Syrian golden and European hamsters were exposed to 14C-labelled cigarette smoke. The deposition of the 14C activity in the respiratory and digestive tracts was determined immediately after the exposure. From the results obtained it can be seen that the distribution of particulate matter between the upper and lower respiratory tract, as well as, between the respiratory and digestive tracts is related to the body weight of the experimental animals.", "contents": "Inhalation experiments with 14C-labelled cigarette smoke. III. Body size dependent distribution of particulate matter in small rodents during cigarette smoke inhalation. Syrian golden and European hamsters were exposed to 14C-labelled cigarette smoke. The deposition of the 14C activity in the respiratory and digestive tracts was determined immediately after the exposure. From the results obtained it can be seen that the distribution of particulate matter between the upper and lower respiratory tract, as well as, between the respiratory and digestive tracts is related to the body weight of the experimental animals.", "PMID": 968917}
{"id": "pubmed23n0032_7425", "title": "[Additive treatment of metastasizing breast cancer with special reference to postmenopausal age (results of a randomized study)].", "content": "Two therapy groups were formed at random out of 82 patients with progressive carcinoma of the breast. One was treated with estrogens, namely 17-ethinyl 1,3,5-estratriene 3,17 diol (SH 8.1083). The second group was treated with depot preparations consisting of a combination of estrogens and gestagens; these patients received 90 mg estradiol valerianate and 300 mg 17-hydroxy-19-norprogesterone caproate (SH 8.0834) weekly. The estrogen therapy resulted in a remission rate of 48% (complete remission 21.1%, partial remission 26.9%). The remission rate of the combination treatment was 46.6% (complete remission 23.3%, partial remission 23.3%). There was no considerable difference between the remission rates produced by the two therapeutik methods. It is remarkable that much more osseous remissions were found under the combination therapy of estrogens and gestagens. There were no substantial differences between the remission rates of the soft-tissue metastases and the pleuropulmonary metastases. In the estrogen group the remissions took 7.7 months, whereas an average remission period of 8.3 months was observed in the estrogen-gestagen group.", "contents": "[Additive treatment of metastasizing breast cancer with special reference to postmenopausal age (results of a randomized study)]. Two therapy groups were formed at random out of 82 patients with progressive carcinoma of the breast. One was treated with estrogens, namely 17-ethinyl 1,3,5-estratriene 3,17 diol (SH 8.1083). The second group was treated with depot preparations consisting of a combination of estrogens and gestagens; these patients received 90 mg estradiol valerianate and 300 mg 17-hydroxy-19-norprogesterone caproate (SH 8.0834) weekly. The estrogen therapy resulted in a remission rate of 48% (complete remission 21.1%, partial remission 26.9%). The remission rate of the combination treatment was 46.6% (complete remission 23.3%, partial remission 23.3%). There was no considerable difference between the remission rates produced by the two therapeutik methods. It is remarkable that much more osseous remissions were found under the combination therapy of estrogens and gestagens. There were no substantial differences between the remission rates of the soft-tissue metastases and the pleuropulmonary metastases. In the estrogen group the remissions took 7.7 months, whereas an average remission period of 8.3 months was observed in the estrogen-gestagen group.", "PMID": 968923}
{"id": "pubmed23n0032_7426", "title": "Time-dependent disappearance of ochratoxin A residues in tissues of bacon pigs.", "content": "Crystalline ochratoxin A was administered to bacon pigs for one month. After termination of toxin exposure the pigs were slaughtered at different intervals and analyses for ochratoxin A residues in four tissues were conducted. Kidneys contained the highest concentrations, and fat the lowest, at each interval. Ochratoxin A disappeared from muscles and fat after 2 weeks, from liver after 3 weeks, and from the kidneys after 4 weeks. The toxin disappeared from tissues exponentially. All the pigs would have passed the meat inspection because no pathologic lesions were developed although tissues contained mycotoxin residues. The results of this study indicate that contamination of meat by ochratoxin A may be avoided by feeding pigs ochratoxin-free feed during the last 4 weeks before slaughter.", "contents": "Time-dependent disappearance of ochratoxin A residues in tissues of bacon pigs. Crystalline ochratoxin A was administered to bacon pigs for one month. After termination of toxin exposure the pigs were slaughtered at different intervals and analyses for ochratoxin A residues in four tissues were conducted. Kidneys contained the highest concentrations, and fat the lowest, at each interval. Ochratoxin A disappeared from muscles and fat after 2 weeks, from liver after 3 weeks, and from the kidneys after 4 weeks. The toxin disappeared from tissues exponentially. All the pigs would have passed the meat inspection because no pathologic lesions were developed although tissues contained mycotoxin residues. The results of this study indicate that contamination of meat by ochratoxin A may be avoided by feeding pigs ochratoxin-free feed during the last 4 weeks before slaughter.", "PMID": 968918}
{"id": "pubmed23n0032_7427", "title": "[Experiences with mammography, plate thermography and cytology as triple diagnosis].", "content": "A report is given on 814 patients who had undergone a mamma examination by means of mammographies, plate thermographies, and, if indicated, punction cytologies. On the basis of our experiences with this triple diagnosis we drafted a preliminary control scheme.", "contents": "[Experiences with mammography, plate thermography and cytology as triple diagnosis]. A report is given on 814 patients who had undergone a mamma examination by means of mammographies, plate thermographies, and, if indicated, punction cytologies. On the basis of our experiences with this triple diagnosis we drafted a preliminary control scheme.", "PMID": 968924}
{"id": "pubmed23n0032_7428", "title": "The incidence and treatment of intestinal and urological complications after combined radiotherapy for uterine carcinomas.", "content": "OPNE HUNDRED AND SIXTY-FOUR PATIENTS WITH GYNECOLOGICAL CARCINOMAS (104 ca colli uteri, 60 cacorporis uteri) were examined retrospectively two to seven years after combined internal-external radiotherapy. Thirty-seven per cent of the patients displayed mild intestinal symptoms during the therapy. The frequency of symptoms remained unchanged after the termination of therapy, though they tended to become more severe. Twenty-five per cent of the patients had urological symptoms during the therapy. Their rate remained the same during the arly follow-up, but increased subsequently, because the frequencies of infections and hematuria had increased. Fourteen patients developed a complication requiring operative treatment five to fifty-seven months after the discontinuation of therapy. Three of them had both intestinal and urological complications, while six had only urological complications. The intestinal complications were repaired with smallgut resections and colostomies, while the urological complications (ureteral strictures) were repaired with different diversion operations with good operative results. Thirteen of the patients with serious complications are alive. One patient had died of profuse rectovaginal bleeding. The mean radiation doses employed were not different in the group with serious complications. Evleven of the patients with complications suffered from different concurrent diseases, which may have diminished their radiation tolerance. The radiotherapy of gynecological malignancies ought to be preformed individually noticing the intestinal reactions during the therapy and the concurrent disease. The value of renography in early detection of urinary tract complications is stressed.", "contents": "The incidence and treatment of intestinal and urological complications after combined radiotherapy for uterine carcinomas. OPNE HUNDRED AND SIXTY-FOUR PATIENTS WITH GYNECOLOGICAL CARCINOMAS (104 ca colli uteri, 60 cacorporis uteri) were examined retrospectively two to seven years after combined internal-external radiotherapy. Thirty-seven per cent of the patients displayed mild intestinal symptoms during the therapy. The frequency of symptoms remained unchanged after the termination of therapy, though they tended to become more severe. Twenty-five per cent of the patients had urological symptoms during the therapy. Their rate remained the same during the arly follow-up, but increased subsequently, because the frequencies of infections and hematuria had increased. Fourteen patients developed a complication requiring operative treatment five to fifty-seven months after the discontinuation of therapy. Three of them had both intestinal and urological complications, while six had only urological complications. The intestinal complications were repaired with smallgut resections and colostomies, while the urological complications (ureteral strictures) were repaired with different diversion operations with good operative results. Thirteen of the patients with serious complications are alive. One patient had died of profuse rectovaginal bleeding. The mean radiation doses employed were not different in the group with serious complications. Evleven of the patients with complications suffered from different concurrent diseases, which may have diminished their radiation tolerance. The radiotherapy of gynecological malignancies ought to be preformed individually noticing the intestinal reactions during the therapy and the concurrent disease. The value of renography in early detection of urinary tract complications is stressed.", "PMID": 968925}
{"id": "pubmed23n0032_7429", "title": "Procedure for field axes measurement, beam indication adjustment, and figure of convergence determination within performance tests for radiation therapy equipment.", "content": "A routine measuring procedure for the verification of radiation field axes and figure of convergence within a spatial resolution of +/- 0.5 mm is described. Measurements are done in two parallel planes in a certain distance before and behind the presumed isocentre. The used test arrangement permits rapid check and controlled adjustment of the alignment of beam or isocentre indicating devices for all isocentic radiation therapy equipment.", "contents": "Procedure for field axes measurement, beam indication adjustment, and figure of convergence determination within performance tests for radiation therapy equipment. A routine measuring procedure for the verification of radiation field axes and figure of convergence within a spatial resolution of +/- 0.5 mm is described. Measurements are done in two parallel planes in a certain distance before and behind the presumed isocentre. The used test arrangement permits rapid check and controlled adjustment of the alignment of beam or isocentre indicating devices for all isocentic radiation therapy equipment.", "PMID": 968926}
{"id": "pubmed23n0032_7430", "title": "Effects of tritiated thymidine on cell population kinetics.", "content": "This paper reports growth-kinetic data of Chinese Hamster tissue culture cells exposed at various times to differnt activities (0.05 muCi/ml up to 10.0 muCi/ml of tritiated thymidine. The analysis of the results aims at the determination of colony-size distribution and to the cellular capacity of reproduction after treatment which is reflected in the cell division spectrum within dialy intervals. The results of the growth-curves represented were converted into integrated curves of colony-size distribution, and their alteration as exponential curves for estimation of the cell cycle as a function of culture time was established. The recovery process and course determination of population kinetics following the 3H-TdR-treatments were evaluated.", "contents": "Effects of tritiated thymidine on cell population kinetics. This paper reports growth-kinetic data of Chinese Hamster tissue culture cells exposed at various times to differnt activities (0.05 muCi/ml up to 10.0 muCi/ml of tritiated thymidine. The analysis of the results aims at the determination of colony-size distribution and to the cellular capacity of reproduction after treatment which is reflected in the cell division spectrum within dialy intervals. The results of the growth-curves represented were converted into integrated curves of colony-size distribution, and their alteration as exponential curves for estimation of the cell cycle as a function of culture time was established. The recovery process and course determination of population kinetics following the 3H-TdR-treatments were evaluated.", "PMID": 968927}
{"id": "pubmed23n0032_7431", "title": "Mutagenicity studies on irradiated potatoes and chlorogenic acid; micronucleus test in rats.", "content": "The micronucleus test was used to study possible mutagenic effects of (1) Irradiated potatoes extracted (a) immediately and (b) after 24-h storage following irradiation; (2) Irradiated and unirradiated chlorogenic acid; and (3) Trenimon. It was observed that irradiated potatoes (groups 1 a, b) and chlorogenic acid (group 2) did not cause mutagenic effects in the bone-marrow cells of rats. The Trenimon group (group 3), which was used as the positive control group, did show significant mutagenic effects.", "contents": "Mutagenicity studies on irradiated potatoes and chlorogenic acid; micronucleus test in rats. The micronucleus test was used to study possible mutagenic effects of (1) Irradiated potatoes extracted (a) immediately and (b) after 24-h storage following irradiation; (2) Irradiated and unirradiated chlorogenic acid; and (3) Trenimon. It was observed that irradiated potatoes (groups 1 a, b) and chlorogenic acid (group 2) did not cause mutagenic effects in the bone-marrow cells of rats. The Trenimon group (group 3), which was used as the positive control group, did show significant mutagenic effects.", "PMID": 968919}
{"id": "pubmed23n0032_7432", "title": "The relationship between the population size and the postirradiation mortality of mice with respect to individual differences in stress reactivity.", "content": "Differences in radiosensitivity, expressed by the death rate up to the 30th day after irradiation by single whole-body exposures in the region of LD50, were followed in mice caged in groups by five, 15 and 25 individuals. No marked differences in the whole=group mortality of differently sized collectives were detected. Preirradiation as well as postirradiation leucocyte counts (after a sublethal irradiation) exhibit, however, differences, which correspond to an assumed different degree of the adrenocortical activity in differently sized collectives. Also the follow-up of the stress reactivity of the animals by measuring the Na/K ratio in 24-hour-samples of urine prior to irradiation demonstrated marked differences between the groups with low number and high number of animals in a cage. By correlating values of this indirect indicator of the adrenocortical activity with manifestations of the individual radiosensitivity it was found that the extremely hyporeactive animals (belonging to groups with five animals per cage) and the extremely hyperreactive ones (belonging to groups with 25 animals per cage) exhibit a markedly higher mortality. The absence of differences in the whole-group mortality of differently sized collectives can be explained by the existence of nonlinear \"U\"-shaped relations between the adrenocortical activity and the individuals' radiosensitivity. The results support the conception on the unfavorable prognostic significance of unbalanced reactions of homeostatic functions of system regulation under conditions of radiation syndrome.", "contents": "The relationship between the population size and the postirradiation mortality of mice with respect to individual differences in stress reactivity. Differences in radiosensitivity, expressed by the death rate up to the 30th day after irradiation by single whole-body exposures in the region of LD50, were followed in mice caged in groups by five, 15 and 25 individuals. No marked differences in the whole=group mortality of differently sized collectives were detected. Preirradiation as well as postirradiation leucocyte counts (after a sublethal irradiation) exhibit, however, differences, which correspond to an assumed different degree of the adrenocortical activity in differently sized collectives. Also the follow-up of the stress reactivity of the animals by measuring the Na/K ratio in 24-hour-samples of urine prior to irradiation demonstrated marked differences between the groups with low number and high number of animals in a cage. By correlating values of this indirect indicator of the adrenocortical activity with manifestations of the individual radiosensitivity it was found that the extremely hyporeactive animals (belonging to groups with five animals per cage) and the extremely hyperreactive ones (belonging to groups with 25 animals per cage) exhibit a markedly higher mortality. The absence of differences in the whole-group mortality of differently sized collectives can be explained by the existence of nonlinear \"U\"-shaped relations between the adrenocortical activity and the individuals' radiosensitivity. The results support the conception on the unfavorable prognostic significance of unbalanced reactions of homeostatic functions of system regulation under conditions of radiation syndrome.", "PMID": 968928}
{"id": "pubmed23n0032_7433", "title": "Fertility and contraceptive practice: Bogota, 1964-74.", "content": "The results of a 1974 survey of 6,692 Bogota women aged 15-49 conducted by the Colombian Association for Population Studies show that, in the last decade, approval of family planning practice increased 34 percent, contraceptive practice nearly doubled, and fertility declined by over 45 percent. Life-table analysis shows unusually high continuation rates for Bogota women. Data concerning crossovers between Bogota's three family planning programs and the commercial sector indicate that the manufacturers of contraceptives benefit from program activities. The study demonstrates, for the first time in Latin America, the feasibility of using a probability sample of the general female population to evaluate family planning programs.", "contents": "Fertility and contraceptive practice: Bogota, 1964-74. The results of a 1974 survey of 6,692 Bogota women aged 15-49 conducted by the Colombian Association for Population Studies show that, in the last decade, approval of family planning practice increased 34 percent, contraceptive practice nearly doubled, and fertility declined by over 45 percent. Life-table analysis shows unusually high continuation rates for Bogota women. Data concerning crossovers between Bogota's three family planning programs and the commercial sector indicate that the manufacturers of contraceptives benefit from program activities. The study demonstrates, for the first time in Latin America, the feasibility of using a probability sample of the general female population to evaluate family planning programs.", "PMID": 968930}
{"id": "pubmed23n0032_7434", "title": "Effectiveness of the Iranian auxiliary midwife in IUD insertion.", "content": "In Iran, a traditional rural midwife is allowed to perform pelvic exams and insert IUD'S under conditions of almost total independence after she has received one month of intensive training in the procedures. The present study compared two groups containing 232 village women each, matched for age and parity. One group had IUDs inserted by rural midwives and the other by physicians or nurse-midwives at ubran clinics. The one-year and two-year closure rates for the two groups were very similar, as were the net closure rates for various categories of termination. No cases of severe complication were known to have occurred in either series. This evidence indicates that a rural midwife, following short, intensive training in the technique, can insert IUDs as safely and effectively as physicians and nurse-midwives.", "contents": "Effectiveness of the Iranian auxiliary midwife in IUD insertion. In Iran, a traditional rural midwife is allowed to perform pelvic exams and insert IUD'S under conditions of almost total independence after she has received one month of intensive training in the procedures. The present study compared two groups containing 232 village women each, matched for age and parity. One group had IUDs inserted by rural midwives and the other by physicians or nurse-midwives at ubran clinics. The one-year and two-year closure rates for the two groups were very similar, as were the net closure rates for various categories of termination. No cases of severe complication were known to have occurred in either series. This evidence indicates that a rural midwife, following short, intensive training in the technique, can insert IUDs as safely and effectively as physicians and nurse-midwives.", "PMID": 968931}
{"id": "pubmed23n0032_7435", "title": "Influence of separation techniques on the distribution and function of lymphocyte subpopulations. A comparison of three techniques.", "content": "The effect, if any, of three different lymphocyte separation techniques on the composition and functional characteristics of the purified cell suspensions has been studied. Each separation technique was shown to yield a cell population with highly specific and reproducible characteristics. The Ficoll-Hypaque technique led to good lymphocyte yields but low yields of sheep erythrocyte-rosetting (E-rosetting) T cells, and the separated cell population responded least well to phytohemagglutinin. The glass sand filtration technique led to lowest overall yield of small lymphocytes and of EAC-rosetting cells. There was significantly lower total yield of E-rosetting T cell as well, but the separated lymphocyte suspension had excellent purity, had relatively high percentage of E-rosetting T cells, and they responded extremely well to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The Technicon separation involving magneticremoval of phagocytic cells by exposure to iron particles consistently led to large yields of small lymphocytes with good purity, the largest total harvests of E-rosetting T cells, as well as EAC-rosetting cells while the separated population had the highest percentage of E-rosetting cells and responded very well to PHA and PWM. These results show that lymphocyte losses during purification are not nonspecific and that the choice of the separation technique profoundly affects the characteristics of the purified lymphocyte population obtainable.", "contents": "Influence of separation techniques on the distribution and function of lymphocyte subpopulations. A comparison of three techniques. The effect, if any, of three different lymphocyte separation techniques on the composition and functional characteristics of the purified cell suspensions has been studied. Each separation technique was shown to yield a cell population with highly specific and reproducible characteristics. The Ficoll-Hypaque technique led to good lymphocyte yields but low yields of sheep erythrocyte-rosetting (E-rosetting) T cells, and the separated cell population responded least well to phytohemagglutinin. The glass sand filtration technique led to lowest overall yield of small lymphocytes and of EAC-rosetting cells. There was significantly lower total yield of E-rosetting T cell as well, but the separated lymphocyte suspension had excellent purity, had relatively high percentage of E-rosetting T cells, and they responded extremely well to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The Technicon separation involving magneticremoval of phagocytic cells by exposure to iron particles consistently led to large yields of small lymphocytes with good purity, the largest total harvests of E-rosetting T cells, as well as EAC-rosetting cells while the separated population had the highest percentage of E-rosetting cells and responded very well to PHA and PWM. These results show that lymphocyte losses during purification are not nonspecific and that the choice of the separation technique profoundly affects the characteristics of the purified lymphocyte population obtainable.", "PMID": 968932}
{"id": "pubmed23n0032_7436", "title": "Cell interaction in tumor allograft immunity. Suppressor cell in the spleen of allogeneic tumor-sensitized mice.", "content": "The cytotoxic activity of lymphoid cells from allogeneic tumor-sensitized mice in three different strain combinations was tested at various intervals after i.p. sensitization. Peritoneal cells (PC) gave the highest cytotoxic index. The cytotoxic activity of the PC was due to both peritoneal lymphocytees (PL) and peritoneal macrophages (PM). Splenic macrophages, on the other hand, exerted an inhibitory effect as evidenced by increased cytotoxic activity of spleen treated with antimacrophages serum (AMS). Increased cytotoxic activity was also found in: (1) the nonadherent fraction of spleen after absorption on glass; (2) the effluent fraction of spleen after nylon wool column filtration; and (3) the medium density band after centrifugation of spleen on a discontinuous bovine serum albumin gradient. The results suggest the presence of an AMS-sensitive, glass- and nylon wool-adherent spleen cell which could have a suppressor role in allogeneic tumor-sensitized mice.", "contents": "Cell interaction in tumor allograft immunity. Suppressor cell in the spleen of allogeneic tumor-sensitized mice. The cytotoxic activity of lymphoid cells from allogeneic tumor-sensitized mice in three different strain combinations was tested at various intervals after i.p. sensitization. Peritoneal cells (PC) gave the highest cytotoxic index. The cytotoxic activity of the PC was due to both peritoneal lymphocytees (PL) and peritoneal macrophages (PM). Splenic macrophages, on the other hand, exerted an inhibitory effect as evidenced by increased cytotoxic activity of spleen treated with antimacrophages serum (AMS). Increased cytotoxic activity was also found in: (1) the nonadherent fraction of spleen after absorption on glass; (2) the effluent fraction of spleen after nylon wool column filtration; and (3) the medium density band after centrifugation of spleen on a discontinuous bovine serum albumin gradient. The results suggest the presence of an AMS-sensitive, glass- and nylon wool-adherent spleen cell which could have a suppressor role in allogeneic tumor-sensitized mice.", "PMID": 968933}
{"id": "pubmed23n0032_7437", "title": "Inhibition of parental tumor colony formation by cells from a semi allogeneic mixed spleen cell reaction.", "content": "Cells capable of inhibiting colony formation by the DBA/2 fibrosarcoma SaD2-AG and mammary adenocarcinoma CaD2 are generated in vitro during semiallogeneic histoincompatibility reactions between DBA/2 and (C3H/He X DBA/2)F1 hybrid spleen cells. These colony-inhibiting cells adhere strongly to plastic, develop from nonadherent precursors, and retain colony-inhibiting ability even after receiving 5,000 rads of ionizing radiation.", "contents": "Inhibition of parental tumor colony formation by cells from a semi allogeneic mixed spleen cell reaction. Cells capable of inhibiting colony formation by the DBA/2 fibrosarcoma SaD2-AG and mammary adenocarcinoma CaD2 are generated in vitro during semiallogeneic histoincompatibility reactions between DBA/2 and (C3H/He X DBA/2)F1 hybrid spleen cells. These colony-inhibiting cells adhere strongly to plastic, develop from nonadherent precursors, and retain colony-inhibiting ability even after receiving 5,000 rads of ionizing radiation.", "PMID": 968934}
{"id": "pubmed23n0032_7438", "title": "The history of the ox warble fly in Cyprus.", "content": "The eradication of ox warble fly from Cyprus is described. Initially, the major part of the eradication consisted of dressing every bovine animal with derris at monthly intervals between December and May (inclusive), but in the final and critical stages when the level of infestation had fallen to 0-2 per cent, it was found that the most efficient and economical approach was to offer a financial reward to any stock-owner reporting the presence of warble fly.", "contents": "The history of the ox warble fly in Cyprus. The eradication of ox warble fly from Cyprus is described. Initially, the major part of the eradication consisted of dressing every bovine animal with derris at monthly intervals between December and May (inclusive), but in the final and critical stages when the level of infestation had fallen to 0-2 per cent, it was found that the most efficient and economical approach was to offer a financial reward to any stock-owner reporting the presence of warble fly.", "PMID": 968948}
{"id": "pubmed23n0032_7439", "title": "Haematological values of Nigerian goats and sheep.", "content": "Haematological parameters were determined in healthy Nigerian breeds of goats and sheep. Most values in the Nigerian goats were similar to those reported for temperate breeds of goats although the haemoglobin concentrations and the MCHC were lower. The haematocrit, haemoglobin concentrations and red cell counts of the West African Dwarf sheep were lower while the MCV were higher than those reported for sheep in the temperate climate. RBC values decreased with age in both Nigerian goats and sheep. Although sex pregnancy appeared to have little or no influence on the erythrocytic values, pregnant ewes had higher haematocrit and haemoglobin values. The low erythrocytic values were attributed to a low but constant parasitic burden which affected the flocks studied. Total leucocyte counts were considerably higher in the Nigerian goats and sheep than those reported for temperate breeds of animals. Young goats had higher total leucocyte counts while pregnant goats had reduced leucocyte counts. The leucocytic values were not affected by age and sex. Pregnant ewes also had higher leucocyte counts than non-pregnant ewes and rams.", "contents": "Haematological values of Nigerian goats and sheep. Haematological parameters were determined in healthy Nigerian breeds of goats and sheep. Most values in the Nigerian goats were similar to those reported for temperate breeds of goats although the haemoglobin concentrations and the MCHC were lower. The haematocrit, haemoglobin concentrations and red cell counts of the West African Dwarf sheep were lower while the MCV were higher than those reported for sheep in the temperate climate. RBC values decreased with age in both Nigerian goats and sheep. Although sex pregnancy appeared to have little or no influence on the erythrocytic values, pregnant ewes had higher haematocrit and haemoglobin values. The low erythrocytic values were attributed to a low but constant parasitic burden which affected the flocks studied. Total leucocyte counts were considerably higher in the Nigerian goats and sheep than those reported for temperate breeds of animals. Young goats had higher total leucocyte counts while pregnant goats had reduced leucocyte counts. The leucocytic values were not affected by age and sex. Pregnant ewes also had higher leucocyte counts than non-pregnant ewes and rams.", "PMID": 968949}
{"id": "pubmed23n0032_7440", "title": "Differences among cattle and farms as related to beef cow reproduction in the eastern plains of Colombia.", "content": "In the tropical savannas of Colombia, the Llanos Orientales, a field survey of 40 beef herds leased by farmers through a cooperative indicated that these herds had mean annual calving rates of 52 and 55 per cent in the Plains and Piedmont zones, respectively. Within zones, very large differences in reproductive rates were found between herds. The differences seemed to be associated with the soils and climates of the areas, condition and weight of cows and breed of cow. Only 9 to 13 per cent of nursing cows were found to be pregnant. Conception was seasonal, occurring mostly in the dry and early rainy seasons. Control of the time of weaning of calves, routine mineral supplementation and improved pasture management appeared to offer immediate possibilities for economically improving output of calves.", "contents": "Differences among cattle and farms as related to beef cow reproduction in the eastern plains of Colombia. In the tropical savannas of Colombia, the Llanos Orientales, a field survey of 40 beef herds leased by farmers through a cooperative indicated that these herds had mean annual calving rates of 52 and 55 per cent in the Plains and Piedmont zones, respectively. Within zones, very large differences in reproductive rates were found between herds. The differences seemed to be associated with the soils and climates of the areas, condition and weight of cows and breed of cow. Only 9 to 13 per cent of nursing cows were found to be pregnant. Conception was seasonal, occurring mostly in the dry and early rainy seasons. Control of the time of weaning of calves, routine mineral supplementation and improved pasture management appeared to offer immediate possibilities for economically improving output of calves.", "PMID": 968950}
{"id": "pubmed23n0032_7441", "title": "Growth and carcass characteristics of cattle and buffalo breeds reared on a dry zone pasture in Sri Lanka (Ceylon).", "content": "Growth and carcass characteristics were measured on calves from three cattle breeds-the Sinhala, Red Sindhi and Friesian-and two buffalo breeds-the local buffalo and Murrah. The growth study was carried out on Brachiaria brizantha pasture over a period of 48 weeks. The two buffalo breeds grew fastest followed by the two Bos indicus breeds. The Friesian cattle grew at the slowest rate. The Sinhala and local buffalo had normal haematocrit values while the values for all other breeds were lower. The Sinhala and local buffalo had the highest carcass dressing percentages  and the highest muscle; bone ratios. Tenderness measured by Warner-Bratzler shear force values and palatability scores by a taste panel were similar for meat from cattle and buffalo.", "contents": "Growth and carcass characteristics of cattle and buffalo breeds reared on a dry zone pasture in Sri Lanka (Ceylon). Growth and carcass characteristics were measured on calves from three cattle breeds-the Sinhala, Red Sindhi and Friesian-and two buffalo breeds-the local buffalo and Murrah. The growth study was carried out on Brachiaria brizantha pasture over a period of 48 weeks. The two buffalo breeds grew fastest followed by the two Bos indicus breeds. The Friesian cattle grew at the slowest rate. The Sinhala and local buffalo had normal haematocrit values while the values for all other breeds were lower. The Sinhala and local buffalo had the highest carcass dressing percentages  and the highest muscle; bone ratios. Tenderness measured by Warner-Bratzler shear force values and palatability scores by a taste panel were similar for meat from cattle and buffalo.", "PMID": 968951}
{"id": "pubmed23n0032_7442", "title": "The maintenance of the argasid tick Ornithodoros moubata (Murray) on embryonated hen eggs.", "content": "A laboratory colony of O. moubata was maintained entirely by feeding on the exposed membranes of embryonated hen eggs at the 17-19th day stage of incubation at 37 degrees C. There was no loss of either vigour or fecundity. The method gave markedly superior results to those obtained by feeding on rabbits, rats, mice and guinea-pigs. More convenient and economical than any alternative, the method has valuable advantages in its applicability to a wide range of pathogens.", "contents": "The maintenance of the argasid tick Ornithodoros moubata (Murray) on embryonated hen eggs. A laboratory colony of O. moubata was maintained entirely by feeding on the exposed membranes of embryonated hen eggs at the 17-19th day stage of incubation at 37 degrees C. There was no loss of either vigour or fecundity. The method gave markedly superior results to those obtained by feeding on rabbits, rats, mice and guinea-pigs. More convenient and economical than any alternative, the method has valuable advantages in its applicability to a wide range of pathogens.", "PMID": 968952}
{"id": "pubmed23n0032_7443", "title": "The effect of calf lymph and bovine red blood cells on in vitro cultivation of Theileria parva-infected lymphoid cells.", "content": "Calf lymph introduced to cultures of Theileria parva-infected lymphoid cells caused a stimulation of both cells and parasites resulting in the formation of rapidly dividing cells and the developmental stages of the parasite. The presence of piroplasms was confirmed when washed bovine red blood corpuscles were added to the culture", "contents": "The effect of calf lymph and bovine red blood cells on in vitro cultivation of Theileria parva-infected lymphoid cells. Calf lymph introduced to cultures of Theileria parva-infected lymphoid cells caused a stimulation of both cells and parasites resulting in the formation of rapidly dividing cells and the developmental stages of the parasite. The presence of piroplasms was confirmed when washed bovine red blood corpuscles were added to the culture", "PMID": 968954}
{"id": "pubmed23n0032_7444", "title": "Effects of head-out water immersion on cardiorespiratory responses to maximal cycling exercise.", "content": "Our objectives were to determine effects of head-out immersion (HOI), scuba breathing, and water temperature on cardiorespiratory responses to maximal aerobic work. Measurements of VO2, VE, and heart rate (HR) were obtained on seven men (27 yr, 177 cm, 67 kg) as they performed the same upright bicycling exercise to exhaustion (4-5 min) in 23 degrees C air and 30 degrees C water. Maximal oxygen uptake (VO2 max) during HOI was 3.18 liters - min-1, which was not statistically different from the mean of 3.29 liters- min-1 in air. When compressed air was breathed via scuba during HOI, VO2 max was 3.12 liters- min-1 and not significantly different from that when room air was breathed and a low-resistance valve in water was used. HOI decreased VE by 15.7 liters - min-1 and HR by 10 beats (b) - min-1. Scuba breathing further reduced VE by 22.0 liters - min-1. Similar measurements were made on four of the subjects after 18 min of HOI in water temperatures of 35,30, and 25 degrees C. Water temperature had no significant affect on VO2 max, although HR was 8 b- min-1 lower in 30 degrees C and 15 b - min-1 lower in 25 degrees C as compared to 35 degrees C water. The results show that VO2 max was not significantly changed by HOI, scuba breathing, or brief exposures to 25, 30, and 35 degrees C water, despite significant reductions that occurred for VE and HR.", "contents": "Effects of head-out water immersion on cardiorespiratory responses to maximal cycling exercise. Our objectives were to determine effects of head-out immersion (HOI), scuba breathing, and water temperature on cardiorespiratory responses to maximal aerobic work. Measurements of VO2, VE, and heart rate (HR) were obtained on seven men (27 yr, 177 cm, 67 kg) as they performed the same upright bicycling exercise to exhaustion (4-5 min) in 23 degrees C air and 30 degrees C water. Maximal oxygen uptake (VO2 max) during HOI was 3.18 liters - min-1, which was not statistically different from the mean of 3.29 liters- min-1 in air. When compressed air was breathed via scuba during HOI, VO2 max was 3.12 liters- min-1 and not significantly different from that when room air was breathed and a low-resistance valve in water was used. HOI decreased VE by 15.7 liters - min-1 and HR by 10 beats (b) - min-1. Scuba breathing further reduced VE by 22.0 liters - min-1. Similar measurements were made on four of the subjects after 18 min of HOI in water temperatures of 35,30, and 25 degrees C. Water temperature had no significant affect on VO2 max, although HR was 8 b- min-1 lower in 30 degrees C and 15 b - min-1 lower in 25 degrees C as compared to 35 degrees C water. The results show that VO2 max was not significantly changed by HOI, scuba breathing, or brief exposures to 25, 30, and 35 degrees C water, despite significant reductions that occurred for VE and HR.", "PMID": 969022}
{"id": "pubmed23n0032_7445", "title": "Diving at diminished atmospheric pressure: air decompression tables for different altitudes.", "content": "Fifty subjects performed 106 simulated dives at a final ambient pressure of 0.7 at (3000 m above sea level). One hundred and forty-three subjects performed 278 actual controlled dives at altitudes 900-1700 m above sea level. From the experience of these dives, air-decompression tables for altitudes 0-3200 m above sea level were calculated. Tables up to 2000 m above sea level were tested on humans under wet conditions.", "contents": "Diving at diminished atmospheric pressure: air decompression tables for different altitudes. Fifty subjects performed 106 simulated dives at a final ambient pressure of 0.7 at (3000 m above sea level). One hundred and forty-three subjects performed 278 actual controlled dives at altitudes 900-1700 m above sea level. From the experience of these dives, air-decompression tables for altitudes 0-3200 m above sea level were calculated. Tables up to 2000 m above sea level were tested on humans under wet conditions.", "PMID": 969023}
{"id": "pubmed23n0032_7446", "title": "Longitudinal study of performance after deep compressions with heliox and He-N2-O2.", "content": "This study concerns three hyperbaric experiments. During SAGITTAIRE iv, compression to 610 msw was accomplished in 11 days with an atmosphere of helium-oxygen. For the two CORAZ experiments, compression to 300 msw was done in 4 hours with He-N2-O2 trimix, varying nitrogen concentration from CORAZI (2.8 ATA N2 =9%) to CORAZ II (1.4 ATA N2=4.5%). Oxygen partial pressure was always maintained at 0.4 ATA except during decompression. The same two subjects participated in all three experiments and underwent the following psychometric tests: manual dexterity, visual choice-reaction time, and number ordination. Results show a large decrement in performance at 610 msw after slow compression (-50% and -47% on the number ordination test, -34% and -24% on the visual choice-reaction time). Test performance was less affected after the rapid compressions to 300 msw, and recuperation to control values occurred within 1 day at 300 msw. Our data show that 1.4 ATA N2 is less detrimental to performance than 2.8 ATA N2 for trimix compressions to 300 msw in 4 hours.", "contents": "Longitudinal study of performance after deep compressions with heliox and He-N2-O2. This study concerns three hyperbaric experiments. During SAGITTAIRE iv, compression to 610 msw was accomplished in 11 days with an atmosphere of helium-oxygen. For the two CORAZ experiments, compression to 300 msw was done in 4 hours with He-N2-O2 trimix, varying nitrogen concentration from CORAZI (2.8 ATA N2 =9%) to CORAZ II (1.4 ATA N2=4.5%). Oxygen partial pressure was always maintained at 0.4 ATA except during decompression. The same two subjects participated in all three experiments and underwent the following psychometric tests: manual dexterity, visual choice-reaction time, and number ordination. Results show a large decrement in performance at 610 msw after slow compression (-50% and -47% on the number ordination test, -34% and -24% on the visual choice-reaction time). Test performance was less affected after the rapid compressions to 300 msw, and recuperation to control values occurred within 1 day at 300 msw. Our data show that 1.4 ATA N2 is less detrimental to performance than 2.8 ATA N2 for trimix compressions to 300 msw in 4 hours.", "PMID": 969024}
{"id": "pubmed23n0032_7447", "title": "Effects of increased O2-N2 pressure and breathing apparatus on respiratory function.", "content": "The ventilatory response of four subjects was measured at rest and various intensities of exercise. Experiments were conducted in a dry pressure chamber (1) at 1 ATA and 4 ATA with the subjects breathing from a low-resistance mouthpiece, and (2) at ATA with the subjects breathing from open-circuit breathing apparatus (Royal Naval Swimmers' Air Breathing Apparatus). At 4 ATA there was significant hypoventilation and hypercapnia, together with an increased tidal volume and lower respiratory frequency. The use of the breathing apparatus tended to amplify these changes in ventilatory response. In addition, the extent of hypercapnia at 4 ATA was related to the exercise intensity. When subjects breathed from a low-resistance mouthpiece, oxygen uptake was significantly greater at 4 ATA than at the surface for the same ergometric work load, but when they breathed from the breathing apparatus, the increase in oxygen uptake was not significant in comparison to surface values. At 4 ATA bradycardia was evident at all levels of exercise but was not affected significantly by the presence of the breathing apparatus.", "contents": "Effects of increased O2-N2 pressure and breathing apparatus on respiratory function. The ventilatory response of four subjects was measured at rest and various intensities of exercise. Experiments were conducted in a dry pressure chamber (1) at 1 ATA and 4 ATA with the subjects breathing from a low-resistance mouthpiece, and (2) at ATA with the subjects breathing from open-circuit breathing apparatus (Royal Naval Swimmers' Air Breathing Apparatus). At 4 ATA there was significant hypoventilation and hypercapnia, together with an increased tidal volume and lower respiratory frequency. The use of the breathing apparatus tended to amplify these changes in ventilatory response. In addition, the extent of hypercapnia at 4 ATA was related to the exercise intensity. When subjects breathed from a low-resistance mouthpiece, oxygen uptake was significantly greater at 4 ATA than at the surface for the same ergometric work load, but when they breathed from the breathing apparatus, the increase in oxygen uptake was not significant in comparison to surface values. At 4 ATA bradycardia was evident at all levels of exercise but was not affected significantly by the presence of the breathing apparatus.", "PMID": 969025}
{"id": "pubmed23n0032_7448", "title": "Critical flicker frequency (CFF) and subjective fatigue during an oxyhelium saturation dive at 62 ATA.", "content": "Two divers spent over 50 hours at 610 msw in a helium-oxygen mixture (PO2:0.38-0.52 ATA). The dive duration was 27 days, including predive stages of confinement, compression, time at maximum pressure, and decompression. The divers were asked to answer 30 questions on their feelings of mental and physical fatigue and to indicate on a nine-point scale their estimation of a general feeling of fatigue. Subjective feelings of fatigue reported in this dive suggested that the divers were in good condition. Hyperbaric arthralgia and physical complaints were reported, especially during decompression, with some postdive persistence, but they should be considered as distinct signs of feelings of fatigue. The critical flicker frequency (CFF), measured throughout the dive for the two divers, showed systematic variations and a relationship between compression and pressure. These variations were grossly parallel to EEG modifications reported in other studies and probably reveal neurophysiological troubles that were not apparent from subjective reports.", "contents": "Critical flicker frequency (CFF) and subjective fatigue during an oxyhelium saturation dive at 62 ATA. Two divers spent over 50 hours at 610 msw in a helium-oxygen mixture (PO2:0.38-0.52 ATA). The dive duration was 27 days, including predive stages of confinement, compression, time at maximum pressure, and decompression. The divers were asked to answer 30 questions on their feelings of mental and physical fatigue and to indicate on a nine-point scale their estimation of a general feeling of fatigue. Subjective feelings of fatigue reported in this dive suggested that the divers were in good condition. Hyperbaric arthralgia and physical complaints were reported, especially during decompression, with some postdive persistence, but they should be considered as distinct signs of feelings of fatigue. The critical flicker frequency (CFF), measured throughout the dive for the two divers, showed systematic variations and a relationship between compression and pressure. These variations were grossly parallel to EEG modifications reported in other studies and probably reveal neurophysiological troubles that were not apparent from subjective reports.", "PMID": 969026}
{"id": "pubmed23n0032_7449", "title": "Reversal of nitrogen narcosis in rats by helium pressure.", "content": "Changes in operant behavior were obtained for rats breathing nitrogen, helium, and both gases combined. Exposure to 12.9 ATA of nitrogen (O2 maintained at normobaric value of 0.2 ATA) produced complete cessation of responding on a fixed-ratio reinforcement schedule for 1 hour. The addition of elevated pressure of helium (12.1, 18.2, and 24.2 ATA) to the 12.9 ATA of nitrogen resulted in a reinstatement of responding during 1-hour hyperbaric exposures. The reinstatement of responding indicated a reversal of the nitrogen effects on behavior by increased helium pressures. The functional changes in behavior appeared to parallel the pressure produced reversal of anesthesia obtained at much higher pressures.", "contents": "Reversal of nitrogen narcosis in rats by helium pressure. Changes in operant behavior were obtained for rats breathing nitrogen, helium, and both gases combined. Exposure to 12.9 ATA of nitrogen (O2 maintained at normobaric value of 0.2 ATA) produced complete cessation of responding on a fixed-ratio reinforcement schedule for 1 hour. The addition of elevated pressure of helium (12.1, 18.2, and 24.2 ATA) to the 12.9 ATA of nitrogen resulted in a reinstatement of responding during 1-hour hyperbaric exposures. The reinstatement of responding indicated a reversal of the nitrogen effects on behavior by increased helium pressures. The functional changes in behavior appeared to parallel the pressure produced reversal of anesthesia obtained at much higher pressures.", "PMID": 969027}
{"id": "pubmed23n0032_7450", "title": "Pressure-reduction limits for rats following steady-state exposures between 6 and 60 ATA.", "content": "The role of pressure reduction in the formation and growth of bubbles is universally recognized and its significance in decompression theory has been accepted. Yet the allowable limits of pressure reduction for man and animal are uncertain. This study sought to evaluate the pressure-reduction limits for rats following steady-state exposures at pressures greater than 1 atm. To define the relationship, 350 albino rats were exposed to 1 of 12 specified pressure levels between 6 and 60 ATA and then abruptly decompressed to a preselected reduced pressure level for observation. The pressure-reduction levels were selected to determine for each saturation-exposure level an ED-50 (i.e. the effective dose that will produce decompression sickness in 50% of the animals). The results demonstrate three consistent findings: (1) there is a linear relationship (r = .99) between the magnitude of a safe pressure reduction and the saturation exposures between 6 and 43 ATA; (2) at pressures greater than 43 ATA, there is a qualitative change in the decompression sickness symptoms and a reduction in the precision of the mathematical relationship (r = .44); and (3) the magnitude of the pressure change required to increase the incidence of decompression sickness from 10% to 90% is directly related to the magnitude of the exposure pressure. The implications of these results for deep operational diving are discussed.", "contents": "Pressure-reduction limits for rats following steady-state exposures between 6 and 60 ATA. The role of pressure reduction in the formation and growth of bubbles is universally recognized and its significance in decompression theory has been accepted. Yet the allowable limits of pressure reduction for man and animal are uncertain. This study sought to evaluate the pressure-reduction limits for rats following steady-state exposures at pressures greater than 1 atm. To define the relationship, 350 albino rats were exposed to 1 of 12 specified pressure levels between 6 and 60 ATA and then abruptly decompressed to a preselected reduced pressure level for observation. The pressure-reduction levels were selected to determine for each saturation-exposure level an ED-50 (i.e. the effective dose that will produce decompression sickness in 50% of the animals). The results demonstrate three consistent findings: (1) there is a linear relationship (r = .99) between the magnitude of a safe pressure reduction and the saturation exposures between 6 and 43 ATA; (2) at pressures greater than 43 ATA, there is a qualitative change in the decompression sickness symptoms and a reduction in the precision of the mathematical relationship (r = .44); and (3) the magnitude of the pressure change required to increase the incidence of decompression sickness from 10% to 90% is directly related to the magnitude of the exposure pressure. The implications of these results for deep operational diving are discussed.", "PMID": 969028}
{"id": "pubmed23n0032_7451", "title": "The response of fish blood cells, particularly thrombocytes, to decompression.", "content": "The effects of decompression on various blood-cell types in chinook salmon (Oncorhynchus tshawytscha) were investigated using a 4-liter hyperbaric chamber. Thrombocytes (platelets) were found to decrease significantly in numbers following lethal and nonlethal decompressions. The response was highly dependent on depth, gas solubility, and rate of decompression, whereby increasing depth or gas solubility caused greater and faster declines of thrombocyte levels. Return of thrombocyte numbers to normal values usually occurred within 48 hours, except after the more severe decompressions where recovery was never fully attained during the sampling period. Erythrocyte levels increased significantly 1 day after a severe decompression, suggesting hemoconcentration. Leucocytes appeared not to respond to decompression; they were not decreased compared to normal levels, although they were significantly decreased compared to levels of the chamber controls in the nonpressurized chamber. The results are discussed in relation to possible involvement of the fish's blood-coagulation system after decompression.", "contents": "The response of fish blood cells, particularly thrombocytes, to decompression. The effects of decompression on various blood-cell types in chinook salmon (Oncorhynchus tshawytscha) were investigated using a 4-liter hyperbaric chamber. Thrombocytes (platelets) were found to decrease significantly in numbers following lethal and nonlethal decompressions. The response was highly dependent on depth, gas solubility, and rate of decompression, whereby increasing depth or gas solubility caused greater and faster declines of thrombocyte levels. Return of thrombocyte numbers to normal values usually occurred within 48 hours, except after the more severe decompressions where recovery was never fully attained during the sampling period. Erythrocyte levels increased significantly 1 day after a severe decompression, suggesting hemoconcentration. Leucocytes appeared not to respond to decompression; they were not decreased compared to normal levels, although they were significantly decreased compared to levels of the chamber controls in the nonpressurized chamber. The results are discussed in relation to possible involvement of the fish's blood-coagulation system after decompression.", "PMID": 969029}
{"id": "pubmed23n0032_7452", "title": "Scanning electron microscopy of normoxic and hyperoxic hyperbaric exposed lungs.", "content": "Lungs from adult guinea pigs exposed to 1 ATA He-O2 with 200 mm Hg PO2 and 20 ATA He-O2 with 200, 400, and 600 mm Hg PO2 were studied with scanning electron microscopy. The appearance of normal alveoli is described. Even before pulmonary O2 toxicity became symptomatic, subtle changes occurred in the alveoli, such as an increase in macrophages and a marked increase in length of alveolar type-II cell microvilli. These changes occurred in animals exposed to 400 mm Hg PO2, heretofore considered below toxic levels. With increased toxic involvement, the number of alveolar type-II cells increased. A thick layer of material appeared in some of the alveoli, obscuring the Kohns pores and type-I and -II cell surfaces. The alveolar-capillary network with underlying erythrocytes was no longer observable. Lungs with the greatest toxic involvement possessed large numbers of macrophages encompassed by a fibrin-like matrix. The alveolar walls were broken down in many instances, and the alveoli were no longer discrete units but took on the appearance of an amorphous mass of lung tissue.", "contents": "Scanning electron microscopy of normoxic and hyperoxic hyperbaric exposed lungs. Lungs from adult guinea pigs exposed to 1 ATA He-O2 with 200 mm Hg PO2 and 20 ATA He-O2 with 200, 400, and 600 mm Hg PO2 were studied with scanning electron microscopy. The appearance of normal alveoli is described. Even before pulmonary O2 toxicity became symptomatic, subtle changes occurred in the alveoli, such as an increase in macrophages and a marked increase in length of alveolar type-II cell microvilli. These changes occurred in animals exposed to 400 mm Hg PO2, heretofore considered below toxic levels. With increased toxic involvement, the number of alveolar type-II cells increased. A thick layer of material appeared in some of the alveoli, obscuring the Kohns pores and type-I and -II cell surfaces. The alveolar-capillary network with underlying erythrocytes was no longer observable. Lungs with the greatest toxic involvement possessed large numbers of macrophages encompassed by a fibrin-like matrix. The alveolar walls were broken down in many instances, and the alveoli were no longer discrete units but took on the appearance of an amorphous mass of lung tissue.", "PMID": 969030}
{"id": "pubmed23n0032_7453", "title": "[Localization of urinary infection after antireflux surgery (author's transl)].", "content": "Bladder wash-out tests were performed in a series of patients after successful antireflux surgery. The majority (65.5%) of infections were localized in the bladder, even if the IVP demonstrated signs of pyelonephritis (renal scarring and calcieal clubbing). 21.3% of the tests demonstrated a participation of the upper urinary tract. The bladder bacteriurias were interpreted as reinfections, and also some of the supravesical bacteriurias. In other cases, this differentiation could not be made.", "contents": "[Localization of urinary infection after antireflux surgery (author's transl)]. Bladder wash-out tests were performed in a series of patients after successful antireflux surgery. The majority (65.5%) of infections were localized in the bladder, even if the IVP demonstrated signs of pyelonephritis (renal scarring and calcieal clubbing). 21.3% of the tests demonstrated a participation of the upper urinary tract. The bladder bacteriurias were interpreted as reinfections, and also some of the supravesical bacteriurias. In other cases, this differentiation could not be made.", "PMID": 969048}
{"id": "pubmed23n0032_7454", "title": "Subcapsular pyelolithotomy for recurrent renal calculi.", "content": "Subcapsular pyelolithotomy was performed in 28 patients who had earlier undergone either one or more operations in the same kidney for renal calculi. This approach was useful in salvaging the kidney even in the presence of dense perirenal and periureteric adhesions obscuring the normal anatomy of these structures. Serious consideration should be given to this procedure before making the decision to perform nephrectomy in such cases. Surgical technique and the results are discussed.", "contents": "Subcapsular pyelolithotomy for recurrent renal calculi. Subcapsular pyelolithotomy was performed in 28 patients who had earlier undergone either one or more operations in the same kidney for renal calculi. This approach was useful in salvaging the kidney even in the presence of dense perirenal and periureteric adhesions obscuring the normal anatomy of these structures. Serious consideration should be given to this procedure before making the decision to perform nephrectomy in such cases. Surgical technique and the results are discussed.", "PMID": 969049}
{"id": "pubmed23n0032_7455", "title": "Effects of prolactin and dihydrotestosterone upon the rat prostate gland.", "content": "The simultaneous subcutaneous injections of dihydrotestosterone (0.5 mg/kg) and/or ovine prolactin (5 or 50 IU/kg) to mature castrate rats for periods of 5-10 days led to an augmentation of prostate gland levels of RNA, DNA and protein. Of the various lobes of the prostate gland, the dorsal portion seemed to be particularly sensitive to the effects of the combined hormone treatment. In general, the highest dose of prolactin (50 IU/kg) and the longer duration of administration (10 days) produced the most consistent augmentation responses between dihydrotestosterone and prolactin as compared to those rats receiving only androgen. These findings reveal that certain lobes of the prostate are more responsive to hormonal interactions, but do not clarify the mechanisms involved in causing such enhancements of prostate cell growth and proliferation.", "contents": "Effects of prolactin and dihydrotestosterone upon the rat prostate gland. The simultaneous subcutaneous injections of dihydrotestosterone (0.5 mg/kg) and/or ovine prolactin (5 or 50 IU/kg) to mature castrate rats for periods of 5-10 days led to an augmentation of prostate gland levels of RNA, DNA and protein. Of the various lobes of the prostate gland, the dorsal portion seemed to be particularly sensitive to the effects of the combined hormone treatment. In general, the highest dose of prolactin (50 IU/kg) and the longer duration of administration (10 days) produced the most consistent augmentation responses between dihydrotestosterone and prolactin as compared to those rats receiving only androgen. These findings reveal that certain lobes of the prostate are more responsive to hormonal interactions, but do not clarify the mechanisms involved in causing such enhancements of prostate cell growth and proliferation.", "PMID": 969050}
{"id": "pubmed23n0032_7456", "title": "[The use of human dura in urology, except as a substitute of urinary bladder (author's transl)].", "content": "The human dura cannot only be used to replace the urinary bladder wall, but also to replace the capsula fibrosa of the kidney for transposition of aberrant vessels in hydronephrosis, for interposition in vesicovaginal fistula, for suspension of the urethra in stress incontinence and to replace the tunica albuginea of the corpus cavernosum penis after resection of plaque in Peyronie's disease.", "contents": "[The use of human dura in urology, except as a substitute of urinary bladder (author's transl)]. The human dura cannot only be used to replace the urinary bladder wall, but also to replace the capsula fibrosa of the kidney for transposition of aberrant vessels in hydronephrosis, for interposition in vesicovaginal fistula, for suspension of the urethra in stress incontinence and to replace the tunica albuginea of the corpus cavernosum penis after resection of plaque in Peyronie's disease.", "PMID": 969051}
{"id": "pubmed23n0032_7457", "title": "Right ovarian vein syndrome with contralateral ovarian cyst.", "content": "A 39-year-old woman with right ovarian vein syndrome accompanied by contralateral ovarian cyst is reported. Removal of the left ovarian cyst with right ureterolysis for removal of the mechanical obstruction gave a good result. It is concluded that the etiology in this case was compression of the ureter by the enlarged ovarian vein, which had an increased blood flow from contralateral venous anastomose.", "contents": "Right ovarian vein syndrome with contralateral ovarian cyst. A 39-year-old woman with right ovarian vein syndrome accompanied by contralateral ovarian cyst is reported. Removal of the left ovarian cyst with right ureterolysis for removal of the mechanical obstruction gave a good result. It is concluded that the etiology in this case was compression of the ureter by the enlarged ovarian vein, which had an increased blood flow from contralateral venous anastomose.", "PMID": 969052}
{"id": "pubmed23n0032_7458", "title": "[Experience with a one-stage hypospadias repair (author's transl)].", "content": "33 single-stage hypospadias repairs are reported, which were performed within 2 years. The operative technique is described. In two cases, fistulas developed and required operative treatment. The average follow-up was 13.5 months. 28 cases showed good functional and cosmetical results. Three cases required additional surgery to improve the cosmetical result. The Allen-Spence-Hoffman-Hall one-stage technique has proved to be suitable to correct a distal hypospadias without chordee.", "contents": "[Experience with a one-stage hypospadias repair (author's transl)]. 33 single-stage hypospadias repairs are reported, which were performed within 2 years. The operative technique is described. In two cases, fistulas developed and required operative treatment. The average follow-up was 13.5 months. 28 cases showed good functional and cosmetical results. Three cases required additional surgery to improve the cosmetical result. The Allen-Spence-Hoffman-Hall one-stage technique has proved to be suitable to correct a distal hypospadias without chordee.", "PMID": 969053}
{"id": "pubmed23n0032_7459", "title": "Leiomyosarcoma of renal vein.", "content": "Primary leiomyosarcoma of the renal vein has not been previously reported in the urologic literature. A case report with long-term survival is described and the literature reviewed.", "contents": "Leiomyosarcoma of renal vein. Primary leiomyosarcoma of the renal vein has not been previously reported in the urologic literature. A case report with long-term survival is described and the literature reviewed.", "PMID": 969071}
{"id": "pubmed23n0032_7460", "title": "Splinting in pyeloplasty.", "content": "Over a ten-year period, 129 patients with idiopathic pelviureteric obstruction were randomly selected for splinted or unsplinted pyeloplasties. Of these groups, the patients with splinted pyeloplasties had a lower incidence of postoperative complications. The long-term results were similar in both groups.", "contents": "Splinting in pyeloplasty. Over a ten-year period, 129 patients with idiopathic pelviureteric obstruction were randomly selected for splinted or unsplinted pyeloplasties. Of these groups, the patients with splinted pyeloplasties had a lower incidence of postoperative complications. The long-term results were similar in both groups.", "PMID": 969072}
{"id": "pubmed23n0032_7461", "title": "Prostatic carcinoma. relationship between primary tumor, histologic grade, and response to chemotherapy.", "content": "This National Cooperative Study has randomly compared the usefulness of 5-fluorouracil (5-FU), cyclophosphamide (Cytoxan) and standard therapy in patients with advanced carcinoma of the prostate (Stage D). All patients studied were endocrine failures and were in progression. Favorable responses were seen with all three treatments regardless of histologic grade. Actual tumor regression occurred only in patients who received either 5-FU or cyclophosphamide. In patients with poorly differentiated or anaplastic tumors treated with cyclophosphamide, progression of disease was significantly less rapid than in patients treated with 5-FU or standard therapy. However, favorable responders could not always be identified in advance on the basis of histologic grade alone.", "contents": "Prostatic carcinoma. relationship between primary tumor, histologic grade, and response to chemotherapy. This National Cooperative Study has randomly compared the usefulness of 5-fluorouracil (5-FU), cyclophosphamide (Cytoxan) and standard therapy in patients with advanced carcinoma of the prostate (Stage D). All patients studied were endocrine failures and were in progression. Favorable responses were seen with all three treatments regardless of histologic grade. Actual tumor regression occurred only in patients who received either 5-FU or cyclophosphamide. In patients with poorly differentiated or anaplastic tumors treated with cyclophosphamide, progression of disease was significantly less rapid than in patients treated with 5-FU or standard therapy. However, favorable responders could not always be identified in advance on the basis of histologic grade alone.", "PMID": 969073}
{"id": "pubmed23n0032_7462", "title": "Simple screening test for significant bacteriuria in urine. Catalase determination by disk flotation method.", "content": "Colony count cultures and catalase determinations were done on 294 urine samples in two series of patients at Royal Victoria Hospital. Thirty-six specimens were reported to be positive by colony count and by catalase determination. A total of 135 specimens found to be negative by colony count were also negative by the disk flotation test. Catalase activity in 4 urines reported as false negative were found to contain 6,000 or less colonies per ml. More than 50 per cent of the false positive results could be attributed to the presence of red blood cells. Data were insufficient to explain the remaining false positive catalase determinations. However, catalase determinations by the disk flotation method proved to be a successful screening test for significant bacteriuria.", "contents": "Simple screening test for significant bacteriuria in urine. Catalase determination by disk flotation method. Colony count cultures and catalase determinations were done on 294 urine samples in two series of patients at Royal Victoria Hospital. Thirty-six specimens were reported to be positive by colony count and by catalase determination. A total of 135 specimens found to be negative by colony count were also negative by the disk flotation test. Catalase activity in 4 urines reported as false negative were found to contain 6,000 or less colonies per ml. More than 50 per cent of the false positive results could be attributed to the presence of red blood cells. Data were insufficient to explain the remaining false positive catalase determinations. However, catalase determinations by the disk flotation method proved to be a successful screening test for significant bacteriuria.", "PMID": 969074}
{"id": "pubmed23n0032_7463", "title": "Problems associated with Gibbons ureteral catheter.", "content": "Two recent cases of bilateral ureteral obstruction secondary to benign retroperitoneal  fibrosis were treated initially with Gibbons indwelling ureteral catheters. Inadequate catheter length, calyceal stone formation, catheter encrustation, and distal migration form the basis for this report.", "contents": "Problems associated with Gibbons ureteral catheter. Two recent cases of bilateral ureteral obstruction secondary to benign retroperitoneal  fibrosis were treated initially with Gibbons indwelling ureteral catheters. Inadequate catheter length, calyceal stone formation, catheter encrustation, and distal migration form the basis for this report.", "PMID": 969075}
{"id": "pubmed23n0032_7464", "title": "Preoperative irradiation and cystectomy in 135 cases of bladder cancer.", "content": "The updated experience of the McGill Teaching Hospitals in treating cancer of the bladder with short course, high-dose radiation and immediate cystectomy is presented. Five-year life table survival for the 135 patients so managed was 43.9 per cent. Survivorship at five years was 34.5 per cent in high-stage disease and  64.1 per cent in low-stage disease. It was concluded that preoperative radiation and surgery as outlined in selected cases remains an effective method of management.", "contents": "Preoperative irradiation and cystectomy in 135 cases of bladder cancer. The updated experience of the McGill Teaching Hospitals in treating cancer of the bladder with short course, high-dose radiation and immediate cystectomy is presented. Five-year life table survival for the 135 patients so managed was 43.9 per cent. Survivorship at five years was 34.5 per cent in high-stage disease and  64.1 per cent in low-stage disease. It was concluded that preoperative radiation and surgery as outlined in selected cases remains an effective method of management.", "PMID": 969076}
{"id": "pubmed23n0032_7465", "title": "Ectopic penile testis.", "content": "A case of a rare form of penile testicular ectopia is reported. The embryology is discussed, and ectopia is differentiated from cryptorchism.", "contents": "Ectopic penile testis. A case of a rare form of penile testicular ectopia is reported. The embryology is discussed, and ectopia is differentiated from cryptorchism.", "PMID": 969077}
{"id": "pubmed23n0032_7466", "title": "Necrotic testicle with increased blood flow on Doppler ultrasonic examination.", "content": "A twelve-year-old male with a five-day history of scrotal swelling and pain had increased blood flow on ultrasonic examination. A testicular flow and scan indicated an ischemic testicle. Surgical exploration revealed complete torsion of the spermatic cord. In chronic cases of torsion, the Doppler stethoscope may give a false negative result because of reactive hyperemia.", "contents": "Necrotic testicle with increased blood flow on Doppler ultrasonic examination. A twelve-year-old male with a five-day history of scrotal swelling and pain had increased blood flow on ultrasonic examination. A testicular flow and scan indicated an ischemic testicle. Surgical exploration revealed complete torsion of the spermatic cord. In chronic cases of torsion, the Doppler stethoscope may give a false negative result because of reactive hyperemia.", "PMID": 969078}
{"id": "pubmed23n0032_7467", "title": "Diagnostic significance of levels of immunoglobulin A in seminal fluid of patients with prostatic disease.", "content": "Immunoglobulin A (IgA) levels in semen and in prostatic fluid of 48 patients with benign and malignant diseases of the prostate were estimated. It is speculated from this small study that the IgA levels rise in benign prostatic hyperplasia; in early adenocarcinoma of the prostate (Stages A and B) the rise if furthered, while in the late stages (Stages C and D) the levels fall.", "contents": "Diagnostic significance of levels of immunoglobulin A in seminal fluid of patients with prostatic disease. Immunoglobulin A (IgA) levels in semen and in prostatic fluid of 48 patients with benign and malignant diseases of the prostate were estimated. It is speculated from this small study that the IgA levels rise in benign prostatic hyperplasia; in early adenocarcinoma of the prostate (Stages A and B) the rise if furthered, while in the late stages (Stages C and D) the levels fall.", "PMID": 969080}
{"id": "pubmed23n0032_7468", "title": "Extraosseous myelomatosis. Clinical presentation with symptoms in lower urinary tract.", "content": "We believe this to be the first case reported of extraosseous myelomatosis seen clincally with lower urinary tract symptoms. Extraskeletal spread of myelomatosis which reportedly occurs in two thirds of cases is discovered postmortem. Immunoglobulin A prevails in this type of myelomatosis. Clinical course and autopsy findings are discussed.", "contents": "Extraosseous myelomatosis. Clinical presentation with symptoms in lower urinary tract. We believe this to be the first case reported of extraosseous myelomatosis seen clincally with lower urinary tract symptoms. Extraskeletal spread of myelomatosis which reportedly occurs in two thirds of cases is discovered postmortem. Immunoglobulin A prevails in this type of myelomatosis. Clinical course and autopsy findings are discussed.", "PMID": 969082}
{"id": "pubmed23n0032_7469", "title": "Nephrogenic metaplasia (adenomatoid tumors) of bladder.", "content": "Two cases of rare nephrogenic metaplasia of the bladder (adenomatoid bladder tumor) support the theory that these tumors originate as an atypical metaplastic response of urothelium to chronic infection or irritation. That these may also represent mesonephric or mesothelial choristomas is considered. Although benign, they may recur. Therapy should consist of antibiotics and transurethral resection or fulguration.", "contents": "Nephrogenic metaplasia (adenomatoid tumors) of bladder. Two cases of rare nephrogenic metaplasia of the bladder (adenomatoid bladder tumor) support the theory that these tumors originate as an atypical metaplastic response of urothelium to chronic infection or irritation. That these may also represent mesonephric or mesothelial choristomas is considered. Although benign, they may recur. Therapy should consist of antibiotics and transurethral resection or fulguration.", "PMID": 969083}
{"id": "pubmed23n0032_7470", "title": "Renal masses with unusual angiographic appearances.", "content": "Despite modern methods in evaluating renal masses a small percentage of cases remain elusive to preoperative diagnosis. The authors discuss ten renal masses with heterogeneous pathology seen at their service within the last two years. An outline of the difficulties in arriving at a correct preoperative diagnosis is discussed. The clinical findings and pathology encountered are evaluated.", "contents": "Renal masses with unusual angiographic appearances. Despite modern methods in evaluating renal masses a small percentage of cases remain elusive to preoperative diagnosis. The authors discuss ten renal masses with heterogeneous pathology seen at their service within the last two years. An outline of the difficulties in arriving at a correct preoperative diagnosis is discussed. The clinical findings and pathology encountered are evaluated.", "PMID": 969084}
{"id": "pubmed23n0032_7471", "title": "Radiologic features of adrenal metastases.", "content": "The incidence of adrenal metastasis is surprisingly high with certain types of malignant neoplasms such as carcinoma of the breast and lung, and melanoma. Since adrenal metastases are usually clinically silent, radiologic findings assume greater importance. The radiologic features found in 21 patients with proved adrenal metastases are presented.", "contents": "Radiologic features of adrenal metastases. The incidence of adrenal metastasis is surprisingly high with certain types of malignant neoplasms such as carcinoma of the breast and lung, and melanoma. Since adrenal metastases are usually clinically silent, radiologic findings assume greater importance. The radiologic features found in 21 patients with proved adrenal metastases are presented.", "PMID": 969085}
{"id": "pubmed23n0032_7472", "title": "Ureteral changes in Gardner's syndrome simulating retroperitoneal fibrosis.", "content": "The development of desmoid tumors in patients with Gardner's syndrome may produce ureteral findings on excretory urogram which, by themselves, are indistinguishable from other causes of retroperitoneal fibrosis.", "contents": "Ureteral changes in Gardner's syndrome simulating retroperitoneal fibrosis. The development of desmoid tumors in patients with Gardner's syndrome may produce ureteral findings on excretory urogram which, by themselves, are indistinguishable from other causes of retroperitoneal fibrosis.", "PMID": 969086}
{"id": "pubmed23n0032_7473", "title": "Ring-shaped calcification of renal mass.", "content": "A case of peripherally calcified renal mass is presented which was interpreted as being a benign, calcified, developmental nephrogenic cyst. A differential diagnosis of calcified renal masses is presented.", "contents": "Ring-shaped calcification of renal mass. A case of peripherally calcified renal mass is presented which was interpreted as being a benign, calcified, developmental nephrogenic cyst. A differential diagnosis of calcified renal masses is presented.", "PMID": 969087}
{"id": "pubmed23n0032_7474", "title": "[Use of gelvisol in the early postoperative period in patients with diseases of the abdominal organs].", "content": "The substance gelvisol obtained in Leningrad Research Institute of Hematology and Blood Transfusion was employed in 198 patients with abdominal organs pathology. There was noted a positive effect of the peroral plasma substitutor on the circulatory blood volume and its components, electrolytic balance, general condition of the operated patients. The authors have elaborated the method of gelvisol administration both per os (30-50 ml each 1-2 hours; up to 1000-2000 ml a day) and in intraintestinal administration via a sound.", "contents": "[Use of gelvisol in the early postoperative period in patients with diseases of the abdominal organs]. The substance gelvisol obtained in Leningrad Research Institute of Hematology and Blood Transfusion was employed in 198 patients with abdominal organs pathology. There was noted a positive effect of the peroral plasma substitutor on the circulatory blood volume and its components, electrolytic balance, general condition of the operated patients. The authors have elaborated the method of gelvisol administration both per os (30-50 ml each 1-2 hours; up to 1000-2000 ml a day) and in intraintestinal administration via a sound.", "PMID": 969130}
{"id": "pubmed23n0032_7475", "title": "[Clinical characteristics, selection of the method and results of treatment of nonspecific spontaneous pneumothorax].", "content": "Under observation were 144 patients, aged from 16 to 74 years, examined and treated for nonspecific spontaneous pneumothorax observed in 161 cases. Most patients (96) were under 40. The development of pneumothorax in most of them might be related with previously suffered diseases of the respiratory tract and lung. The clinical picture of spontaneous pneumothorax and its therapy are described.", "contents": "[Clinical characteristics, selection of the method and results of treatment of nonspecific spontaneous pneumothorax]. Under observation were 144 patients, aged from 16 to 74 years, examined and treated for nonspecific spontaneous pneumothorax observed in 161 cases. Most patients (96) were under 40. The development of pneumothorax in most of them might be related with previously suffered diseases of the respiratory tract and lung. The clinical picture of spontaneous pneumothorax and its therapy are described.", "PMID": 969144}
{"id": "pubmed23n0032_7476", "title": "[Selection of the methods of functional examination of patients with lung diseases in the preoperative period].", "content": "The authors report indications and contraindications to using different methods of functional diagnosis of the ventilatory and circulatory systems in patients with lung diseases in the postoperative period. It is felt that in each particular case different methods of functional diagnosis should be employed differentially, taking into consideration individual peculiarities of the patient and his affection. It is necessary to use the methods that would provide the maximum useful information, but not to use them following the principle of transfer from simple to more complex procedures.", "contents": "[Selection of the methods of functional examination of patients with lung diseases in the preoperative period]. The authors report indications and contraindications to using different methods of functional diagnosis of the ventilatory and circulatory systems in patients with lung diseases in the postoperative period. It is felt that in each particular case different methods of functional diagnosis should be employed differentially, taking into consideration individual peculiarities of the patient and his affection. It is necessary to use the methods that would provide the maximum useful information, but not to use them following the principle of transfer from simple to more complex procedures.", "PMID": 969145}
{"id": "pubmed23n0032_7477", "title": "[Certain properties of the extravasate effused into pleural cavity after thoracic surgery].", "content": "Data on morphological and biochemical properties of the postoperative extravasate are reported in 102 patients in different terms postoperatively. There is a reduced number of erythrocytes, amount of hemoglobin and thrombocytes in the extravasate. The number of leucocytes in the latter corresponds to their amount in peripheral blood. Free hemoglobin ranged from 6 to 125 mg%, and its concentration depends on traumatism and duration of surgery. There is no fibrinogen in the extravasate and a decreased fibrinolytic activity. Bacterial contamination of the extravasate is dependent on the character of a surgical procedure. The extravasate sterility is supported by its apyrogenic and nontoxic action on rabbits and mice. Determination of the amount of leucocytes, sugar, alpha-2 and beta golbulins in the extravasate 18 hours following the operation is of a diagnostic value for early recognition of inflammation in the pleural cavity.", "contents": "[Certain properties of the extravasate effused into pleural cavity after thoracic surgery]. Data on morphological and biochemical properties of the postoperative extravasate are reported in 102 patients in different terms postoperatively. There is a reduced number of erythrocytes, amount of hemoglobin and thrombocytes in the extravasate. The number of leucocytes in the latter corresponds to their amount in peripheral blood. Free hemoglobin ranged from 6 to 125 mg%, and its concentration depends on traumatism and duration of surgery. There is no fibrinogen in the extravasate and a decreased fibrinolytic activity. Bacterial contamination of the extravasate is dependent on the character of a surgical procedure. The extravasate sterility is supported by its apyrogenic and nontoxic action on rabbits and mice. Determination of the amount of leucocytes, sugar, alpha-2 and beta golbulins in the extravasate 18 hours following the operation is of a diagnostic value for early recognition of inflammation in the pleural cavity.", "PMID": 969146}
{"id": "pubmed23n0032_7478", "title": "[Causes of recurrences of mitral stenosis].", "content": "The authors present an analysis of the late results of closed mitral commissurotomy in 364 patients (maximum terms of observation--17 years). Main causes of recurrent mitral stenosis were recognized: activation of a rheumatic process (50%), nonspecific scarring processes (27%), inadequate commissurotomies (11.1%) and others (11.1%). The routine antirheumatic therapy (bicillin+salicylates) was found to be ineffective in prophylaxis against mitral restenoses. Recurrent mitral stenosis proved to occur more frequently in patients operated upon in young age (under 30).", "contents": "[Causes of recurrences of mitral stenosis]. The authors present an analysis of the late results of closed mitral commissurotomy in 364 patients (maximum terms of observation--17 years). Main causes of recurrent mitral stenosis were recognized: activation of a rheumatic process (50%), nonspecific scarring processes (27%), inadequate commissurotomies (11.1%) and others (11.1%). The routine antirheumatic therapy (bicillin+salicylates) was found to be ineffective in prophylaxis against mitral restenoses. Recurrent mitral stenosis proved to occur more frequently in patients operated upon in young age (under 30).", "PMID": 969147}
{"id": "pubmed23n0032_7479", "title": "[Possibilities of using the internal jugular vein as an autovenous transplant in surgical treatment of portal hypertension].", "content": "As an autovenous graft in surgical treatment of portal hypertension the authors suggest to use the one obtained by means of resection of one of internal jugular veins. Based on studies of the literature data and personal experiments with 500 anatomical preparations of the skull and 49 fresh cadavers, the authors substantiate physiological permissibility of resection of one of internal jugular veins in the absence of marked asymmetry of internal jugular foramena of the cranial base, that is a criterion of symmetric venous outflow from the cranial cavity and of the possibility of its redistribution. The mentioned operative technic was employed by the authors clinically in one patient with a extrahepatic form of portal hypertension, subjected previously to splenectomy. The result of this intervention after a year and a half was estimated as being quite favourable.", "contents": "[Possibilities of using the internal jugular vein as an autovenous transplant in surgical treatment of portal hypertension]. As an autovenous graft in surgical treatment of portal hypertension the authors suggest to use the one obtained by means of resection of one of internal jugular veins. Based on studies of the literature data and personal experiments with 500 anatomical preparations of the skull and 49 fresh cadavers, the authors substantiate physiological permissibility of resection of one of internal jugular veins in the absence of marked asymmetry of internal jugular foramena of the cranial base, that is a criterion of symmetric venous outflow from the cranial cavity and of the possibility of its redistribution. The mentioned operative technic was employed by the authors clinically in one patient with a extrahepatic form of portal hypertension, subjected previously to splenectomy. The result of this intervention after a year and a half was estimated as being quite favourable.", "PMID": 969148}
{"id": "pubmed23n0032_7480", "title": "[Immediate and remote results of treatment of hepatic echinococcosis].", "content": "The immediate results of treatment in 493 patients, in whom 573 operations were accomplished, are analysed. Postoperatively, complications developed in 47.5%. The longterm results were studied in 284 patients within the the terms from 1 to 25 years. The signs of hepatitis were observed in 72, chronic fistulas--in 68, postoperative hernias--in 13, a decreased working capacity and disability--in 32. Recurrences were noted in 52 cases, including true recurrences in 11.", "contents": "[Immediate and remote results of treatment of hepatic echinococcosis]. The immediate results of treatment in 493 patients, in whom 573 operations were accomplished, are analysed. Postoperatively, complications developed in 47.5%. The longterm results were studied in 284 patients within the the terms from 1 to 25 years. The signs of hepatitis were observed in 72, chronic fistulas--in 68, postoperative hernias--in 13, a decreased working capacity and disability--in 32. Recurrences were noted in 52 cases, including true recurrences in 11.", "PMID": 969149}
{"id": "pubmed23n0032_7481", "title": "[Blood coagulation and blood electrolytes in obstructive jaundice].", "content": "An examination of 76 patients with obstructive jaundice of cholelithic and tumor origin indicated an association of manifest hypocoagulation properties of blood with the absolute kalium deficiency in blood. In 54 patients postoperatively following restoration of bile passage in the bowel a progressive decrease in kalium concentration and an increase of sodium in erythrocytes were associated with a simultaneous enhancement of coagulation properties, and vice versa an increased kalium and decreased sodium content in erythrocytes was associated with a hypocaugulation tendency. It is evident that activation or suppression of hemocoagulation is dependent on the state of multiple factors of blood coagulation system in altered electrolytic medium and on eletrolytic reconstruction of erythrocytes.", "contents": "[Blood coagulation and blood electrolytes in obstructive jaundice]. An examination of 76 patients with obstructive jaundice of cholelithic and tumor origin indicated an association of manifest hypocoagulation properties of blood with the absolute kalium deficiency in blood. In 54 patients postoperatively following restoration of bile passage in the bowel a progressive decrease in kalium concentration and an increase of sodium in erythrocytes were associated with a simultaneous enhancement of coagulation properties, and vice versa an increased kalium and decreased sodium content in erythrocytes was associated with a hypocaugulation tendency. It is evident that activation or suppression of hemocoagulation is dependent on the state of multiple factors of blood coagulation system in altered electrolytic medium and on eletrolytic reconstruction of erythrocytes.", "PMID": 969150}
{"id": "pubmed23n0032_7482", "title": "[Diffuse polyposis of the large intestine as a precancerous condition].", "content": "In 86 (49.4%) of 173 patients cancer of the colon of various localizations against the background of diffuse polyposis was diagnosed, rectal involvement being noted in 50%, cancer involvement of the cecum involvement of the cecum and ascending colon--in 5.4% and 7.2% accordingly. These data made it possible to revise an existing concept of preservation of the rectum in radicalsurgery for diffuse polyposis in favour of remaining right portions of the colon.", "contents": "[Diffuse polyposis of the large intestine as a precancerous condition]. In 86 (49.4%) of 173 patients cancer of the colon of various localizations against the background of diffuse polyposis was diagnosed, rectal involvement being noted in 50%, cancer involvement of the cecum involvement of the cecum and ascending colon--in 5.4% and 7.2% accordingly. These data made it possible to revise an existing concept of preservation of the rectum in radicalsurgery for diffuse polyposis in favour of remaining right portions of the colon.", "PMID": 969151}
{"id": "pubmed23n0032_7483", "title": "[Healing of suppurative wounds after heteroperitoneal, gauze and resin-rinse methods of drainage].", "content": "It is shown that preserved parietal peritoneum of the cattle shows necrolytic and dehydrating properties, contributes to prompt wound clearance from fibrinopurulent exudate, thus shortening the time of the phase of hydration, and as a week stimulator it stimulates regenerative processes, contributing to accelerated dehydration phase and healing of the wound with formation of a tender scar without any signs of inflammation, the regeneration of the activated epidermis being more perfect. Moreover, the metabolic processes in the wound are actiyated, that is manifested by a more intensive. Feulgen reaction for DNA, rapid accumulation of RNA in cells and glycoproteids and glycogen--in intercellular substance. Gauze and rubber drainages fail to possess such properties.", "contents": "[Healing of suppurative wounds after heteroperitoneal, gauze and resin-rinse methods of drainage]. It is shown that preserved parietal peritoneum of the cattle shows necrolytic and dehydrating properties, contributes to prompt wound clearance from fibrinopurulent exudate, thus shortening the time of the phase of hydration, and as a week stimulator it stimulates regenerative processes, contributing to accelerated dehydration phase and healing of the wound with formation of a tender scar without any signs of inflammation, the regeneration of the activated epidermis being more perfect. Moreover, the metabolic processes in the wound are actiyated, that is manifested by a more intensive. Feulgen reaction for DNA, rapid accumulation of RNA in cells and glycoproteids and glycogen--in intercellular substance. Gauze and rubber drainages fail to possess such properties.", "PMID": 969152}
{"id": "pubmed23n0032_7484", "title": "[Thermography in differential diagnosis of lymphostasis in the lower limbs].", "content": "The authors report the data concerning the use of lymphography and thermography with the purpose of establishing the differential diagnosis in 42 patients with edema of the lower limbs of a different origin. A comparative estimation of different methods of the differential diagnosis indicated the advantages of infra-red thermography.", "contents": "[Thermography in differential diagnosis of lymphostasis in the lower limbs]. The authors report the data concerning the use of lymphography and thermography with the purpose of establishing the differential diagnosis in 42 patients with edema of the lower limbs of a different origin. A comparative estimation of different methods of the differential diagnosis indicated the advantages of infra-red thermography.", "PMID": 969153}
{"id": "pubmed23n0032_7485", "title": "[Effect of mechanized work on the indicators of traumatism among lumberjacks].", "content": "The author has considered trauma from the point of view of three main indices: frequency, gravity and average duration of incapacity of each patient in days. An important conclusion is made that further progressive replacement of manual labour by mechanized one will influence positively the basic indices of traumatism in timber industry.", "contents": "[Effect of mechanized work on the indicators of traumatism among lumberjacks]. The author has considered trauma from the point of view of three main indices: frequency, gravity and average duration of incapacity of each patient in days. An important conclusion is made that further progressive replacement of manual labour by mechanized one will influence positively the basic indices of traumatism in timber industry.", "PMID": 969154}
{"id": "pubmed23n0032_7486", "title": "[Evaluation of the effectiveness of orthopedic manipulation of inveterate traumatic hip dislocation].", "content": "The authors present an analysis of the results of operative treatment for inveterate femoral dislocations in 13 patients, in 6 of them open reduction of the dislocation was performed, in 6--arthrodesis of the coxa, in 1--subtrochanteric osteotomy. The late results within the terms from 3 to 15 years were followed up in 9 operated patients, 5 of them were previously subjected to operative reduction of the dislocation and 4--to arthrodesis. It is the authors' opinion that, taking into account the late results following operative reduction of inveterate femoral dislocation, the operation of arthrodesis of the coxa is felt to be more rational in such cases.", "contents": "[Evaluation of the effectiveness of orthopedic manipulation of inveterate traumatic hip dislocation]. The authors present an analysis of the results of operative treatment for inveterate femoral dislocations in 13 patients, in 6 of them open reduction of the dislocation was performed, in 6--arthrodesis of the coxa, in 1--subtrochanteric osteotomy. The late results within the terms from 3 to 15 years were followed up in 9 operated patients, 5 of them were previously subjected to operative reduction of the dislocation and 4--to arthrodesis. It is the authors' opinion that, taking into account the late results following operative reduction of inveterate femoral dislocation, the operation of arthrodesis of the coxa is felt to be more rational in such cases.", "PMID": 969155}
{"id": "pubmed23n0032_7487", "title": "[Operative and postoperative neurological complications in heart surgery].", "content": "Intraoperative and postoperative neurologic complications in cardiosurgical patients refer to grave cerebral affections which aggravate markedly the operative issue and prognosis for patients' life. In 525 operations on the heart and major vessels neurologic complications were noted in 47 patients. In 21 of them death was directly caused by these complications. The character of cerebral lesions (brain hypoxia, acute disorders in the cerebral circulation) and their frequency were conditioned by the kind of operative interventions and characteristic features of the cardiovascular pathology. Routine prophylactic measures and their results are described.", "contents": "[Operative and postoperative neurological complications in heart surgery]. Intraoperative and postoperative neurologic complications in cardiosurgical patients refer to grave cerebral affections which aggravate markedly the operative issue and prognosis for patients' life. In 525 operations on the heart and major vessels neurologic complications were noted in 47 patients. In 21 of them death was directly caused by these complications. The character of cerebral lesions (brain hypoxia, acute disorders in the cerebral circulation) and their frequency were conditioned by the kind of operative interventions and characteristic features of the cardiovascular pathology. Routine prophylactic measures and their results are described.", "PMID": 969156}
{"id": "pubmed23n0032_7488", "title": "[Surgical treatment of trochanteric fractures of the femur].", "content": "Till 1971 in the clinic the treatment of trochanteric fractures was mostly conservative. Application of this method resulted in numerous complications and high mortality rate (33%). Among 363 patients admitted to the clinic during this period surgical therapy was employed only in 13 patients (3.5%). Within the recent 3 years indications to surgical therapy were considerably extended: of 111 admitted patients 41 were treated surgically (37%). The greatest number of operations was made in patients aged 71-90 years old. For osteosynthesis pin-screw with diaphyseal pad was employed. The results obtained are described.", "contents": "[Surgical treatment of trochanteric fractures of the femur]. Till 1971 in the clinic the treatment of trochanteric fractures was mostly conservative. Application of this method resulted in numerous complications and high mortality rate (33%). Among 363 patients admitted to the clinic during this period surgical therapy was employed only in 13 patients (3.5%). Within the recent 3 years indications to surgical therapy were considerably extended: of 111 admitted patients 41 were treated surgically (37%). The greatest number of operations was made in patients aged 71-90 years old. For osteosynthesis pin-screw with diaphyseal pad was employed. The results obtained are described.", "PMID": 969157}
{"id": "pubmed23n0032_7489", "title": "[Electric stimulation as a method of acceleration of healing of crural bone fractures].", "content": "Having used the method of electrostimulation of the fracture site with direct current of 10--50 mA in patients with crural fractures, the authors noted a more rapid callus formation. The latter is supported by clinical data and roentgenograms. The method results in shortening of the terms of recovery in patients treated conservatively and surgically. It is believed that this method may be recommended for a wider clinical use in crural fractures.", "contents": "[Electric stimulation as a method of acceleration of healing of crural bone fractures]. Having used the method of electrostimulation of the fracture site with direct current of 10--50 mA in patients with crural fractures, the authors noted a more rapid callus formation. The latter is supported by clinical data and roentgenograms. The method results in shortening of the terms of recovery in patients treated conservatively and surgically. It is believed that this method may be recommended for a wider clinical use in crural fractures.", "PMID": 969158}
{"id": "pubmed23n0032_7490", "title": "[Compression-distraction osteosynthesis of pseudarthroses and ununited fractures of the long tubular bones].", "content": "The authors presents an analysis of clinical observations over 72 patients with pseudarthrosis and nonunited fractures of long tubular bones, in whom 73 operations of compression-distraction osteosynthesis were performed. All these patients are engaged in coal industry. For treatment the Ilizarov compression-distraction apparatus was employed in 62 patients, the Gudushauri apparatus--in 5, the apparatus of personal author's design--in 6. the results of treatment are described.", "contents": "[Compression-distraction osteosynthesis of pseudarthroses and ununited fractures of the long tubular bones]. The authors presents an analysis of clinical observations over 72 patients with pseudarthrosis and nonunited fractures of long tubular bones, in whom 73 operations of compression-distraction osteosynthesis were performed. All these patients are engaged in coal industry. For treatment the Ilizarov compression-distraction apparatus was employed in 62 patients, the Gudushauri apparatus--in 5, the apparatus of personal author's design--in 6. the results of treatment are described.", "PMID": 969159}
{"id": "pubmed23n0032_7491", "title": "[Counter-lateral compression in oblique pseudarthroses of diaphyses of the tubular bones of the lower limbs].", "content": "The author reports the results of treatment in 58 patients with oblique aplastic pseudarthrosis of tubular bones diaphyses of the lower limbs. For treatment extrafocal with counter-lateral compression by means of pins with bearing surfaces. By the present time nearly in all patients (52 of 58) with the mentioned complications such kind of treatment resulted in rehabilitation of the main motion-bearing function of the extremity.", "contents": "[Counter-lateral compression in oblique pseudarthroses of diaphyses of the tubular bones of the lower limbs]. The author reports the results of treatment in 58 patients with oblique aplastic pseudarthrosis of tubular bones diaphyses of the lower limbs. For treatment extrafocal with counter-lateral compression by means of pins with bearing surfaces. By the present time nearly in all patients (52 of 58) with the mentioned complications such kind of treatment resulted in rehabilitation of the main motion-bearing function of the extremity.", "PMID": 969160}
{"id": "pubmed23n0032_7492", "title": "[Gas exchange in burns].", "content": "Under study was the function of ventilation, gas metabolism, acid-base balance, ECG in 140 patients with burns associated with or without clinical manifestations of shock reaction. It is found that shock is not characterized by any specific changes in the cardiopulmonary system. The disturbances observed in patients with manifest burn shock and in those without shock reaction differ only in the degree of pronouncement and consist in dyspnea with a hypoventilation regimen, high level of basic metabolism, decreased reserve potentialities of the body, metabolic acidosis, diffuse changes in the myocardium. Some proper recommendations are given.", "contents": "[Gas exchange in burns]. Under study was the function of ventilation, gas metabolism, acid-base balance, ECG in 140 patients with burns associated with or without clinical manifestations of shock reaction. It is found that shock is not characterized by any specific changes in the cardiopulmonary system. The disturbances observed in patients with manifest burn shock and in those without shock reaction differ only in the degree of pronouncement and consist in dyspnea with a hypoventilation regimen, high level of basic metabolism, decreased reserve potentialities of the body, metabolic acidosis, diffuse changes in the myocardium. Some proper recommendations are given.", "PMID": 969161}
{"id": "pubmed23n0032_7493", "title": "[Changes of peripheral blood flow in patients with cancer of the lungs, esophagus and cardia under the effect of anesthesia and surgery].", "content": "The authors report the data on changes in peripheral circulation, depending on the kind of anesthesia, the amount of operative blood loss, alterations in circulating blood volume and localization of tumor in patients with cancer of the lung, esophagus and cardia. Totally, 35 patients (15--lung cancer, 10--esophageal cancer, 10--cancer of the cardia) were explored. In 25 cases fluothane was used as the main anesthetic, in 10--neuroleptanalgesia. The state of peripheral circulation was estimated by the period semielimination of radioactive xenon-133 from the muscle depot. It was found that alterations in peripheral circulation were not dependent on tumor localization, the use of fluothane and neuroleptanalgesia is associated with similar changes in peripheral blood flow and, finely, an increased loss of blood results in more pronounced alterations in peripheral circulation.", "contents": "[Changes of peripheral blood flow in patients with cancer of the lungs, esophagus and cardia under the effect of anesthesia and surgery]. The authors report the data on changes in peripheral circulation, depending on the kind of anesthesia, the amount of operative blood loss, alterations in circulating blood volume and localization of tumor in patients with cancer of the lung, esophagus and cardia. Totally, 35 patients (15--lung cancer, 10--esophageal cancer, 10--cancer of the cardia) were explored. In 25 cases fluothane was used as the main anesthetic, in 10--neuroleptanalgesia. The state of peripheral circulation was estimated by the period semielimination of radioactive xenon-133 from the muscle depot. It was found that alterations in peripheral circulation were not dependent on tumor localization, the use of fluothane and neuroleptanalgesia is associated with similar changes in peripheral blood flow and, finely, an increased loss of blood results in more pronounced alterations in peripheral circulation.", "PMID": 969162}
{"id": "pubmed23n0032_7494", "title": "[Respiratory function during peridural analgesia].", "content": "By means of spirography the ventilatory function was studied under conditions of peridural analgesia in 73 patients with suppurative and neoplastic diseases of the lung pre- and postoperatively, as well as in closed chest injuries. In 6 patients being in asthmatic state the effect of peridural analgesia is estimated clinically.", "contents": "[Respiratory function during peridural analgesia]. By means of spirography the ventilatory function was studied under conditions of peridural analgesia in 73 patients with suppurative and neoplastic diseases of the lung pre- and postoperatively, as well as in closed chest injuries. In 6 patients being in asthmatic state the effect of peridural analgesia is estimated clinically.", "PMID": 969163}
{"id": "pubmed23n0032_7495", "title": "[Use of heterogenic peritoneum in surgical treatment of ankylosis of the temporomandibular joint].", "content": "Thirty patients were operated upon after the authors' technic. In 23 patients the mandibular branch was made thinner after the method described, and caps from the heterogenous tissue were employed. The mandibular motion was found to be quite adequate, terms of the follow-up from 2 to 18 years. In 7 patients oblique osteotomy of the mandibular branch was performed with interposition of the fissure with a heterogenous peritoneum leaf. The mandibular motion was satisfactory.", "contents": "[Use of heterogenic peritoneum in surgical treatment of ankylosis of the temporomandibular joint]. Thirty patients were operated upon after the authors' technic. In 23 patients the mandibular branch was made thinner after the method described, and caps from the heterogenous tissue were employed. The mandibular motion was found to be quite adequate, terms of the follow-up from 2 to 18 years. In 7 patients oblique osteotomy of the mandibular branch was performed with interposition of the fissure with a heterogenous peritoneum leaf. The mandibular motion was satisfactory.", "PMID": 969164}
{"id": "pubmed23n0032_7496", "title": "Visceral lesions in racoons naturally infected with Heterobilharzia americana.", "content": "Three free-ranging raccoons caught in southeastern Florida were found to be infected by Heterobilharzia americana. The livers were firm with rough surfaces and stellate patterns of periportal fibrosis. The portal tracts had excessive mature fibrous connective tissue and occasional ovum and necrotic fluke granulomas. There were also ovum granulomas with concomitant lesions in small and large intestine, pancreas, and lung. Numerous other parasites were seen.", "contents": "Visceral lesions in racoons naturally infected with Heterobilharzia americana. Three free-ranging raccoons caught in southeastern Florida were found to be infected by Heterobilharzia americana. The livers were firm with rough surfaces and stellate patterns of periportal fibrosis. The portal tracts had excessive mature fibrous connective tissue and occasional ovum and necrotic fluke granulomas. There were also ovum granulomas with concomitant lesions in small and large intestine, pancreas, and lung. Numerous other parasites were seen.", "PMID": 969165}
{"id": "pubmed23n0032_7497", "title": "Hemorrhagic necrosis of the central nervous system. A spontaneous disease of fetal hamsters.", "content": "During studies of transplacental virus infections in random bred hamsters purchased with timed pregnancies from three commercial dealers, spontaneous hemorrhagic necrosis of the central nervous system was seen in fetuses harvested near term. Ninety-seven pregnant hamsters from three colonies were examined during a 6-month period; this condition was seen in 25 of 41, 19 of 36, and five of 20 litters. Hamsters from another commercial colony were received, housed, and fed under the same conditions, but remained free of the disease. The pathological process was characterized by multiple spreading zones of edema, malacia, and hemorrhage. Lesions were restricted to neural tissues, including the retina and internal ear. Neuroepithelial proliferation with rosette formation, accompanying the destructive process, constituted a striking reparative response. No inflammatory reaction or cytopathic effects suggestive of virus-induced disease were seen. Studies on the cause of this condition were negative at the time when the disease disappeared spontaneously.", "contents": "Hemorrhagic necrosis of the central nervous system. A spontaneous disease of fetal hamsters. During studies of transplacental virus infections in random bred hamsters purchased with timed pregnancies from three commercial dealers, spontaneous hemorrhagic necrosis of the central nervous system was seen in fetuses harvested near term. Ninety-seven pregnant hamsters from three colonies were examined during a 6-month period; this condition was seen in 25 of 41, 19 of 36, and five of 20 litters. Hamsters from another commercial colony were received, housed, and fed under the same conditions, but remained free of the disease. The pathological process was characterized by multiple spreading zones of edema, malacia, and hemorrhage. Lesions were restricted to neural tissues, including the retina and internal ear. Neuroepithelial proliferation with rosette formation, accompanying the destructive process, constituted a striking reparative response. No inflammatory reaction or cytopathic effects suggestive of virus-induced disease were seen. Studies on the cause of this condition were negative at the time when the disease disappeared spontaneously.", "PMID": 969166}
{"id": "pubmed23n0032_7498", "title": "Renal carcinoma in the dog.", "content": "Thirty-three cases of canine renal carcinoma reported between 1907 and 1972 are reviewed, and clinical and pathological features of nine other cases seen in Bristol between 1966 and 1975 are described. Clinical features frequently include nonspecific signs of anorexia and weight loss. The age range of affected dogs varies from 3 to 15 years, with a mean of 7.1 years. Male dogs appear to be affected more frequently than females, but there is no breed predilection. The clinical behaviour of canine renal carcinoma is variable, with 4 years the longest survival time after nephrectomy. The primary tumours characteristically are large and unilateral and occupy most of one pole of a kidney. Metastatic spread can involve a wide range of organs, the commonest being lymph nodes and lung. Solid, tubular and papillary patterns are common. Clear cell carcinoma is uncommon.", "contents": "Renal carcinoma in the dog. Thirty-three cases of canine renal carcinoma reported between 1907 and 1972 are reviewed, and clinical and pathological features of nine other cases seen in Bristol between 1966 and 1975 are described. Clinical features frequently include nonspecific signs of anorexia and weight loss. The age range of affected dogs varies from 3 to 15 years, with a mean of 7.1 years. Male dogs appear to be affected more frequently than females, but there is no breed predilection. The clinical behaviour of canine renal carcinoma is variable, with 4 years the longest survival time after nephrectomy. The primary tumours characteristically are large and unilateral and occupy most of one pole of a kidney. Metastatic spread can involve a wide range of organs, the commonest being lymph nodes and lung. Solid, tubular and papillary patterns are common. Clear cell carcinoma is uncommon.", "PMID": 969167}
{"id": "pubmed23n0032_7499", "title": "[Epidermoid bronchial carcinoma in dogs and cats].", "content": "In seven cats and four dogs of different ages, an epidermoid pulmonary carcinoma has been described. This tumour originates from the orifices or the proximal sections of the ducts of the bronchial glands. The carcinoma, which usually shows no keratinization, penetrates the bronchial mucosa, submucosa and the peribronchial tissue. There is no mucus. Occasionally in the peribronchial neoplastic areas there are adenoid formations, comparable to the combined carcinoma of the WHO classification of lung tumours. Metastases have been restricted to the bronchial lymph nodes.", "contents": "[Epidermoid bronchial carcinoma in dogs and cats]. In seven cats and four dogs of different ages, an epidermoid pulmonary carcinoma has been described. This tumour originates from the orifices or the proximal sections of the ducts of the bronchial glands. The carcinoma, which usually shows no keratinization, penetrates the bronchial mucosa, submucosa and the peribronchial tissue. There is no mucus. Occasionally in the peribronchial neoplastic areas there are adenoid formations, comparable to the combined carcinoma of the WHO classification of lung tumours. Metastases have been restricted to the bronchial lymph nodes.", "PMID": 969168}
{"id": "pubmed23n0032_7500", "title": "Histology of air sac lesions induced in chickens by contact exposure to Mycoplasma synoviae.", "content": "Chickens were housed when 1 day old with chickens experimentally infected with Mycoplasma synoviae. Air sacs from 16 of the 2-week-old and eight of the 3-week-old contact-exposed chickens were given gross lesion scores and embedded in glycol methacrylate for slide preparation. Histologic lesions in air sacs with gross scores of 0-2 were mild edema resulting in a two to eightfold increase in air sac thickness, capillary proliferation, and exudate consisting largely of heterophils and necrotic debris. Histologic lesions in air sacs with gross scores of 3 and 4 were marked hyperplasia of epithelial cells and diffuse infiltration of the air sac connective tissue by mononuclear cells. Nine of 10 air sacs with gross scores of 0 had no histologic evidence of inflammation. The most severe histologic lesions were in those air sacs with gross scores of 4. The glycol methacrylate procedure resulted in 2-mum sections with excellent cellular detail.", "contents": "Histology of air sac lesions induced in chickens by contact exposure to Mycoplasma synoviae. Chickens were housed when 1 day old with chickens experimentally infected with Mycoplasma synoviae. Air sacs from 16 of the 2-week-old and eight of the 3-week-old contact-exposed chickens were given gross lesion scores and embedded in glycol methacrylate for slide preparation. Histologic lesions in air sacs with gross scores of 0-2 were mild edema resulting in a two to eightfold increase in air sac thickness, capillary proliferation, and exudate consisting largely of heterophils and necrotic debris. Histologic lesions in air sacs with gross scores of 3 and 4 were marked hyperplasia of epithelial cells and diffuse infiltration of the air sac connective tissue by mononuclear cells. Nine of 10 air sacs with gross scores of 0 had no histologic evidence of inflammation. The most severe histologic lesions were in those air sacs with gross scores of 4. The glycol methacrylate procedure resulted in 2-mum sections with excellent cellular detail.", "PMID": 969169}
{"id": "pubmed23n0032_7501", "title": "Homogeneous joint transplantation in sheep.", "content": "An experimental study on the homogeneous transplantation of the major part of the lateral and medial femoral trochleae and trochlear groove with articular cartilage was carried out in the School of Veterinary Medicine, Pahlavi University, Shiraz. The results of postoperative clinical examinations and regular radiographical observations are described. Finally, the pathological findings and surgico-pathological observations are noted. The advantages of using the technique described and its possible clinical applications are discussed.", "contents": "Homogeneous joint transplantation in sheep. An experimental study on the homogeneous transplantation of the major part of the lateral and medial femoral trochleae and trochlear groove with articular cartilage was carried out in the School of Veterinary Medicine, Pahlavi University, Shiraz. The results of postoperative clinical examinations and regular radiographical observations are described. Finally, the pathological findings and surgico-pathological observations are noted. The advantages of using the technique described and its possible clinical applications are discussed.", "PMID": 969173}
{"id": "pubmed23n0032_7502", "title": "Comparison of pregnancy rate in heifers and suckler cows after progesterone or prostaglandin treatments.", "content": "The pregnancy rate in 321 Friesian dairy replacement heifers was not different following two inseminations on a fixed time basis when oestrus was synchronised with either a 12-day progesterone treatment using silastic coils or a double injection regimen of a synthetic analogue of prostaglandin f2alpha (chloprostenol). There was a significantly higher (P less than 0-01) pregnancy rate following insemination when 143 Hereford-cross beef suckler cows were treated with the 12-day progesterone treatment (55 per cent pregnant) in comparison to the pregnancy rate following insemination of 131 cows receiving the double injection of cloprostenol 12 days apart (32 per cent pregnant). The ovarian activity at the start of treatment affected pregnancy rate following the cloprostenol regimen but not following the progesterone regimen. In suckler cows in these trials where ovarian activity was classified at the start of treatment, 30 per cent had inactive ovaries, indicating the magnitude of the problems of synchronising oestrus in beef suckler cows.", "contents": "Comparison of pregnancy rate in heifers and suckler cows after progesterone or prostaglandin treatments. The pregnancy rate in 321 Friesian dairy replacement heifers was not different following two inseminations on a fixed time basis when oestrus was synchronised with either a 12-day progesterone treatment using silastic coils or a double injection regimen of a synthetic analogue of prostaglandin f2alpha (chloprostenol). There was a significantly higher (P less than 0-01) pregnancy rate following insemination when 143 Hereford-cross beef suckler cows were treated with the 12-day progesterone treatment (55 per cent pregnant) in comparison to the pregnancy rate following insemination of 131 cows receiving the double injection of cloprostenol 12 days apart (32 per cent pregnant). The ovarian activity at the start of treatment affected pregnancy rate following the cloprostenol regimen but not following the progesterone regimen. In suckler cows in these trials where ovarian activity was classified at the start of treatment, 30 per cent had inactive ovaries, indicating the magnitude of the problems of synchronising oestrus in beef suckler cows.", "PMID": 969174}
{"id": "pubmed23n0032_7503", "title": "The effect of orally administered histamine on the weight gain and development of gizzard lesions in chicks.", "content": "The results of a large scale trial confirmed preliminary findings that the feeding to chicks of a diet containing 4 mg/g of histamine can result in the production of localised lesions in the gizzard and depressed growth rate. This finding supports an earlier suggestion that when dietary fish meal is associated with gizzard erosion the condition is mediated, in part, by the histamine produced by certain types of bacterial spoilage of fish protein.", "contents": "The effect of orally administered histamine on the weight gain and development of gizzard lesions in chicks. The results of a large scale trial confirmed preliminary findings that the feeding to chicks of a diet containing 4 mg/g of histamine can result in the production of localised lesions in the gizzard and depressed growth rate. This finding supports an earlier suggestion that when dietary fish meal is associated with gizzard erosion the condition is mediated, in part, by the histamine produced by certain types of bacterial spoilage of fish protein.", "PMID": 969179}
{"id": "pubmed23n0032_7504", "title": "Reproduction of the ewe and female goat in the province of Fars, Iran.", "content": "Over a period of 12 months, 1006 ovine and 1129 caprine genitalia were collected from an abattoir. 57-5 per cent of the sheep and 89 per cent of the goat specimens were gravid of which 26-9 per cent and 30-2 per cent, respectively, of the total were estimated to be over two months of gestational age. Transuterine migration of the early embryo was a feature in both species although it was more frequent in the goat. Signs of fertilisation failure or embryonic death was most frequently observed if associated with multiple ovulations. Three ovine and two caprine fetuses had severe developmental abnormalities, and a total of 30 specimens had either congenital or acquired abnormalities of the ovaries and uterus.", "contents": "Reproduction of the ewe and female goat in the province of Fars, Iran. Over a period of 12 months, 1006 ovine and 1129 caprine genitalia were collected from an abattoir. 57-5 per cent of the sheep and 89 per cent of the goat specimens were gravid of which 26-9 per cent and 30-2 per cent, respectively, of the total were estimated to be over two months of gestational age. Transuterine migration of the early embryo was a feature in both species although it was more frequent in the goat. Signs of fertilisation failure or embryonic death was most frequently observed if associated with multiple ovulations. Three ovine and two caprine fetuses had severe developmental abnormalities, and a total of 30 specimens had either congenital or acquired abnormalities of the ovaries and uterus.", "PMID": 969180}
{"id": "pubmed23n0032_7505", "title": "The relationship between changes in bodyweight plasma glucose and fertility in beef cows.", "content": "Long term experiments were carried out to determine the influence of varying energy intakes on the levels of blood components in lactating beef cows. Lowering the dietary energy intake to 0-95x maintenance produced a fall in bodyweight which continued throughout a 10 week feeding period. There was an initial fall in the plasma glucose level. After four weeks the plasma glucose level began to rise, and at the end of the 10 week period it had almost reached its original level. When bodyweight and plasma glucose were falling cows were infertile, when bodyweight was falling and plasma glucose was rising cows were fertile.", "contents": "The relationship between changes in bodyweight plasma glucose and fertility in beef cows. Long term experiments were carried out to determine the influence of varying energy intakes on the levels of blood components in lactating beef cows. Lowering the dietary energy intake to 0-95x maintenance produced a fall in bodyweight which continued throughout a 10 week feeding period. There was an initial fall in the plasma glucose level. After four weeks the plasma glucose level began to rise, and at the end of the 10 week period it had almost reached its original level. When bodyweight and plasma glucose were falling cows were infertile, when bodyweight was falling and plasma glucose was rising cows were fertile.", "PMID": 969181}
{"id": "pubmed23n0032_7506", "title": "Neck lesions in the teeth of cats.", "content": "Teeth and whole jaws of cats suffering from painful cavitation of the necks of postcanine teeth were investigated histologically. It was found that the lesions were not caries but a type of progressive subgingival osteoclastic resorption.", "contents": "Neck lesions in the teeth of cats. Teeth and whole jaws of cats suffering from painful cavitation of the necks of postcanine teeth were investigated histologically. It was found that the lesions were not caries but a type of progressive subgingival osteoclastic resorption.", "PMID": 969190}
{"id": "pubmed23n0032_7507", "title": "Experimental and field studies with thiophanate in pigs.", "content": "Thiophanate, administered at a dosage of 50 mg per kg to artifically infected pigs, removed 96 to 99 per cent of adult Oesophagostomum spp, Hyostrongylus rubidus and Trichuris suis. Activity was also high against larval stages of these nematodes, except for 26-day-old T suis. Thiophanate also showed ovicidal and larvicidal activity against H rubidus and Oesophagostomum spp. At 50 mg per kg thiophanate administered alone was inactive against Ascaris suum and Metastrongylus apri, the former species also being refractory at 200 mg per kg. Field trials confirmed these efficacy results in naturally infected animals. Pellet formulations providing mean dosages of 63 mg thiophanate per kg for adult pigs and 75 mg thiophanate per kg with 83 mg piperazine base per kg for growing pigs were highly effective in reducing the faecal output of Oesophagostomum spp, H rubidus and T suis eggs. In growing pigs, A suum was controlled by the thiophanate/piperazine product. No palatability or tolerance problems were observed when thiophanate or thiophanate/piperazine mixtures were administered at recommended dosage or multiples thereof in experimental or field studies.", "contents": "Experimental and field studies with thiophanate in pigs. Thiophanate, administered at a dosage of 50 mg per kg to artifically infected pigs, removed 96 to 99 per cent of adult Oesophagostomum spp, Hyostrongylus rubidus and Trichuris suis. Activity was also high against larval stages of these nematodes, except for 26-day-old T suis. Thiophanate also showed ovicidal and larvicidal activity against H rubidus and Oesophagostomum spp. At 50 mg per kg thiophanate administered alone was inactive against Ascaris suum and Metastrongylus apri, the former species also being refractory at 200 mg per kg. Field trials confirmed these efficacy results in naturally infected animals. Pellet formulations providing mean dosages of 63 mg thiophanate per kg for adult pigs and 75 mg thiophanate per kg with 83 mg piperazine base per kg for growing pigs were highly effective in reducing the faecal output of Oesophagostomum spp, H rubidus and T suis eggs. In growing pigs, A suum was controlled by the thiophanate/piperazine product. No palatability or tolerance problems were observed when thiophanate or thiophanate/piperazine mixtures were administered at recommended dosage or multiples thereof in experimental or field studies.", "PMID": 969196}
{"id": "pubmed23n0032_7508", "title": "Mycoplasmas and ovine keratoconjunctivitis.", "content": "The clinical course of an outbreak of keratoconjunctivitis in housed lambs and their dams was followed. Signs were transient generally and became severe in only a small proportion of lambs. The outbreak became most obvious when the lambs were 46 to 55 days old, when 46.9 per cent were affected. Mycoplasma conjunctivae isolations, confirmed by comparison with the type strain by biochemical and serological reactions, increased to 62.1 per cent of all eyes swabbed, but no correlation could be demonstrated between presence of the organism and clinical status. The reasons for this are discussed. Mycoplasma ovipneumoniae was also recovered from the eyes of a small number of lambs. Instillation of a broth culture of M conjunctivae into the conjunctival sacs of four hoggs produced a transient keratoconjunctivitis similar to that observed in the field, but no effect was observed in animals inoculated intravenously. M conjunctivae may therefore be the aetiological agent of non-follicular infectious ovine keratoconjunctivitis, although further work in gnotobiotic or specific pathogen free lambs is required to establish the fact beyond doubt.", "contents": "Mycoplasmas and ovine keratoconjunctivitis. The clinical course of an outbreak of keratoconjunctivitis in housed lambs and their dams was followed. Signs were transient generally and became severe in only a small proportion of lambs. The outbreak became most obvious when the lambs were 46 to 55 days old, when 46.9 per cent were affected. Mycoplasma conjunctivae isolations, confirmed by comparison with the type strain by biochemical and serological reactions, increased to 62.1 per cent of all eyes swabbed, but no correlation could be demonstrated between presence of the organism and clinical status. The reasons for this are discussed. Mycoplasma ovipneumoniae was also recovered from the eyes of a small number of lambs. Instillation of a broth culture of M conjunctivae into the conjunctival sacs of four hoggs produced a transient keratoconjunctivitis similar to that observed in the field, but no effect was observed in animals inoculated intravenously. M conjunctivae may therefore be the aetiological agent of non-follicular infectious ovine keratoconjunctivitis, although further work in gnotobiotic or specific pathogen free lambs is required to establish the fact beyond doubt.", "PMID": 969200}
{"id": "pubmed23n0032_7509", "title": "Type II ostertagiasis in adult cattle.", "content": "During the spring of 1975 outbreaks of ostertagiasis affecting adult cattle were recorded from several beef herds. Two of these outbreaks, one involving an autumn calving herd and the other spring calving, were investigated in detail. The clinical, biochemical, haematological, parasitological and pathological findings are described and were similar to those characteristic of type II ostertagiasis in immature cattle.", "contents": "Type II ostertagiasis in adult cattle. During the spring of 1975 outbreaks of ostertagiasis affecting adult cattle were recorded from several beef herds. Two of these outbreaks, one involving an autumn calving herd and the other spring calving, were investigated in detail. The clinical, biochemical, haematological, parasitological and pathological findings are described and were similar to those characteristic of type II ostertagiasis in immature cattle.", "PMID": 969201}
{"id": "pubmed23n0032_7510", "title": "[Late results of gamma-teletherapy for lung cancer].", "content": "As a result of distance gamma-therapy on Rocus-123 machine in patients with pulmonary cancer in stage II-IV, the survival for a year period and longer was noted in 57.5 +/- 4.5%, over 3 years--in 19.5 +/- 3.6%; over 5 years--in 7.2+/- 2.4%. An average life survival was 21.6 +/- 1.8%.", "contents": "[Late results of gamma-teletherapy for lung cancer]. As a result of distance gamma-therapy on Rocus-123 machine in patients with pulmonary cancer in stage II-IV, the survival for a year period and longer was noted in 57.5 +/- 4.5%, over 3 years--in 19.5 +/- 3.6%; over 5 years--in 7.2+/- 2.4%. An average life survival was 21.6 +/- 1.8%.", "PMID": 969339}
{"id": "pubmed23n0032_7511", "title": "[Lung cancer in chronic inflammatory processes of the bronchi and pulmonary tissue].", "content": "An attempt was made to determine the frequency of lung cancer occurrence in different inflammatory processes in the bronchi and pulmonary tissue. 100 cases of lung cancer were studied according to case reports and autopsy finding, the main tumor node and metastases were explored histologically. The data obtained indicated that in 2.2% of cases the development of cancer was preceded by influenza with residual phenomenon such as purulent bronchitis or pneumonia, in 2.3% of cases cancer occurrence was found to be dependent on chronic pneumonia. In 12.1% of cases cancer has arisen in fibrous-focal or cirrhotic tuberculosis with the presence of old caverns; in 14.7% of cases its development was related with pneumosclerosis and bronchiectases, and in 17.8% of cases it developed against the background of purulent bronchitis.", "contents": "[Lung cancer in chronic inflammatory processes of the bronchi and pulmonary tissue]. An attempt was made to determine the frequency of lung cancer occurrence in different inflammatory processes in the bronchi and pulmonary tissue. 100 cases of lung cancer were studied according to case reports and autopsy finding, the main tumor node and metastases were explored histologically. The data obtained indicated that in 2.2% of cases the development of cancer was preceded by influenza with residual phenomenon such as purulent bronchitis or pneumonia, in 2.3% of cases cancer occurrence was found to be dependent on chronic pneumonia. In 12.1% of cases cancer has arisen in fibrous-focal or cirrhotic tuberculosis with the presence of old caverns; in 14.7% of cases its development was related with pneumosclerosis and bronchiectases, and in 17.8% of cases it developed against the background of purulent bronchitis.", "PMID": 969340}
{"id": "pubmed23n0032_7512", "title": "[Use of olivomycin in the treatment of cancer of the uterine cervix].", "content": "Olivomycin was used locally as 0.2% ointment (20-40 tampons) in 39 cervical cancer cases (I stage--27 patients, II--8, III--1 and IV--2 patients) and in 2 vaginal cancer cases (II stage). There was noted a visual disappearance of the neoplasm in 24 cases, the decrease up to 1/3 of initial tumor dimensions in 11 cases, up to 1/2-2/3--in 5 cases. There was no effect in one case of cervix adenocarcinoma. An extended extirpation of the uterus with its adnexa was carried out after the treatment in 36 cases, in 13 of which no histological signs of tumor were found. Two patients are being under observation, because they refused the operation in view of the complete disappearance of the tumor.", "contents": "[Use of olivomycin in the treatment of cancer of the uterine cervix]. Olivomycin was used locally as 0.2% ointment (20-40 tampons) in 39 cervical cancer cases (I stage--27 patients, II--8, III--1 and IV--2 patients) and in 2 vaginal cancer cases (II stage). There was noted a visual disappearance of the neoplasm in 24 cases, the decrease up to 1/3 of initial tumor dimensions in 11 cases, up to 1/2-2/3--in 5 cases. There was no effect in one case of cervix adenocarcinoma. An extended extirpation of the uterus with its adnexa was carried out after the treatment in 36 cases, in 13 of which no histological signs of tumor were found. Two patients are being under observation, because they refused the operation in view of the complete disappearance of the tumor.", "PMID": 969341}
{"id": "pubmed23n0032_7513", "title": "[Antitumor activity of the lymphocytes of BALB/c and C57BL mice during methylcholanthrene carcinogenesis].", "content": "By means of the elaborated by the authors method of inhibition by lymphocytes of spheroids production by tumor cells a quantitative estimation of their antitumor activity is given in mice BALB/c and C57BL in methylcholanthrene carcinogenesis, Lymphoid cells in mice BALB/c proved to be more active functionally than lymphocytes in mice C57BL. Antitumor action of the lymphocytes is high enough at early stages of blastoma development, lymphocytes from remote lymph nodes being mostly active. At terminal stage the effector action of lymphocytes is diminished.", "contents": "[Antitumor activity of the lymphocytes of BALB/c and C57BL mice during methylcholanthrene carcinogenesis]. By means of the elaborated by the authors method of inhibition by lymphocytes of spheroids production by tumor cells a quantitative estimation of their antitumor activity is given in mice BALB/c and C57BL in methylcholanthrene carcinogenesis, Lymphoid cells in mice BALB/c proved to be more active functionally than lymphocytes in mice C57BL. Antitumor action of the lymphocytes is high enough at early stages of blastoma development, lymphocytes from remote lymph nodes being mostly active. At terminal stage the effector action of lymphocytes is diminished.", "PMID": 969342}
{"id": "pubmed23n0032_7514", "title": "[Morphologic changes in the urinary bladder of mice induced by metabolites of tryptophan and tyrosine].", "content": "45-day-old 229 mice of lines C57Bl and CC57Br were injected subcutaneously water solutions of tryptophan and thyrosine metabolites 2.5 mg twice a week, the total dosage being 45-50 mg per mouse. For the control mice of the same line were taken. Within the period from 3.5 to 19 months following injection of the metabolites in the urinary bladder of animals, there were observed focal proliferates of the epithelium not infrequently with its atypism, and also lymphoid infiltration of submucous and muscle layers, reticulosarcomas, papillomas, precancerous conditions and cancer. The question of the importance of endogenous blastomogenic substances in the development of \"spontaneous\" tumors of the urinary bladder in man is discussed.", "contents": "[Morphologic changes in the urinary bladder of mice induced by metabolites of tryptophan and tyrosine]. 45-day-old 229 mice of lines C57Bl and CC57Br were injected subcutaneously water solutions of tryptophan and thyrosine metabolites 2.5 mg twice a week, the total dosage being 45-50 mg per mouse. For the control mice of the same line were taken. Within the period from 3.5 to 19 months following injection of the metabolites in the urinary bladder of animals, there were observed focal proliferates of the epithelium not infrequently with its atypism, and also lymphoid infiltration of submucous and muscle layers, reticulosarcomas, papillomas, precancerous conditions and cancer. The question of the importance of endogenous blastomogenic substances in the development of \"spontaneous\" tumors of the urinary bladder in man is discussed.", "PMID": 969343}
{"id": "pubmed23n0032_7515", "title": "[Influence of phenobarbital on the immunodepressive action of antitumor substances].", "content": "In the experiments on mice, immunized by mutton erythrocytes, the authors have studied the effect of phenobarbital, in the doses activating drug-metabolizing hepatic systems, on immunodepressive action of imurane, 6-mercaptopurine and cyclophosphane. It has been found that phenobarbital eliminates the immunodepression and reduces considerably the toxicity of the mentioned cytostatics. Under these conditions imurane and 6-mercaptopurine in certain doses render an immunostimulative instead of immunodepressive effect.", "contents": "[Influence of phenobarbital on the immunodepressive action of antitumor substances]. In the experiments on mice, immunized by mutton erythrocytes, the authors have studied the effect of phenobarbital, in the doses activating drug-metabolizing hepatic systems, on immunodepressive action of imurane, 6-mercaptopurine and cyclophosphane. It has been found that phenobarbital eliminates the immunodepression and reduces considerably the toxicity of the mentioned cytostatics. Under these conditions imurane and 6-mercaptopurine in certain doses render an immunostimulative instead of immunodepressive effect.", "PMID": 969346}
{"id": "pubmed23n0032_7516", "title": "[Venous blood leukoconcentrate of oncologic patients following radiation therapy].", "content": "Venous blood leucoconcentrate was studied in 48 patients with malignant neoplasms of the genitalia before and after radiotherapy. The decrease in total number of leucocytes on account of the decreased lymphocytes number and young undifferentiated formed elements of blood was observed; a relative neutrophylosis, monocytosis, eosinophilia as well as lymphopenia developed. The study of venous blood leucoconcentrate in oncological patients characterizes more precisely the state of peripheral blood than the routine method of investigation.", "contents": "[Venous blood leukoconcentrate of oncologic patients following radiation therapy]. Venous blood leucoconcentrate was studied in 48 patients with malignant neoplasms of the genitalia before and after radiotherapy. The decrease in total number of leucocytes on account of the decreased lymphocytes number and young undifferentiated formed elements of blood was observed; a relative neutrophylosis, monocytosis, eosinophilia as well as lymphopenia developed. The study of venous blood leucoconcentrate in oncological patients characterizes more precisely the state of peripheral blood than the routine method of investigation.", "PMID": 969347}
{"id": "pubmed23n0032_7517", "title": "[Late results of lung cancer therapy (according to material from the Kazakh Scientific Research Institute of Oncology and Radiology)].", "content": "The remote results in 295 patients subjected to radical surgical therapy are analysed, in 95 of these patients during two postoperative years 426 courses of chemotherapy were conducted. In squamous cell cancer of the lung 48 patients were administered methotrexate (90 mg per course), 18 patients with adenocarcinoma--5-fluoruracil, 29 patients with undifferentiated cancer-cyclophosphane (3 g of each substance). The comparison of 3 and 5-year results depending on the stage, extent of surgical intervention and histological form of the tumor, indicated a considerable prolongation of life terms in patients, subjected to the combined treatment, as compared with solely surgical one.", "contents": "[Late results of lung cancer therapy (according to material from the Kazakh Scientific Research Institute of Oncology and Radiology)]. The remote results in 295 patients subjected to radical surgical therapy are analysed, in 95 of these patients during two postoperative years 426 courses of chemotherapy were conducted. In squamous cell cancer of the lung 48 patients were administered methotrexate (90 mg per course), 18 patients with adenocarcinoma--5-fluoruracil, 29 patients with undifferentiated cancer-cyclophosphane (3 g of each substance). The comparison of 3 and 5-year results depending on the stage, extent of surgical intervention and histological form of the tumor, indicated a considerable prolongation of life terms in patients, subjected to the combined treatment, as compared with solely surgical one.", "PMID": 969348}
{"id": "pubmed23n0032_7518", "title": "[Recognition of metastases to the liver in lung cancer patients].", "content": "The results of the complex treatment of 441 lung cancer patients are analysed, in whom scanning of the liver was performed to exclude a possible metastatic invasion. 72 patients (16.3%) died in signs of metastatic involvement of the liver: in 33 of them the diagnosis was established based on the data of scanning. 30 of them were suspected of metastases in the liver due to scanning findings, and only in 10 cases scannograms were normal. In 14 of the last 40 patients repeat scannograms revealed metastases. Of the total number of 46 patients with metastases, revealed scannographically, only 15 showed clinical and scannographic signs of tumor involvement of the liver, whereas in the remaining 31--primary clinical signs of the liver involvement were absent, and metastases were first detected by radiologic studies. It is concluded, that it is rational to include scanning of the liver in the complex of the mandatory exploration in patients with lung cancer.", "contents": "[Recognition of metastases to the liver in lung cancer patients]. The results of the complex treatment of 441 lung cancer patients are analysed, in whom scanning of the liver was performed to exclude a possible metastatic invasion. 72 patients (16.3%) died in signs of metastatic involvement of the liver: in 33 of them the diagnosis was established based on the data of scanning. 30 of them were suspected of metastases in the liver due to scanning findings, and only in 10 cases scannograms were normal. In 14 of the last 40 patients repeat scannograms revealed metastases. Of the total number of 46 patients with metastases, revealed scannographically, only 15 showed clinical and scannographic signs of tumor involvement of the liver, whereas in the remaining 31--primary clinical signs of the liver involvement were absent, and metastases were first detected by radiologic studies. It is concluded, that it is rational to include scanning of the liver in the complex of the mandatory exploration in patients with lung cancer.", "PMID": 969350}
{"id": "pubmed23n0032_7519", "title": "[State of the exocrine function of the pancreas during human aging].", "content": "A study of the exacrinous function of the pancreas made in 205 healthy persons aged from 18 to 90 has evidenced that in ageing there takes place in the duodenal mixture of juices a statistically significant decline in the activity of trypsin and of its inhibitor, lipase, amylase, in the level of bicarbonates and in the volume of the juice along with an increase in the content of chlorides. In ageing more marked changes become apparent following application of the agents stimulating the pancreatic secretion of the secretin and pancreozymin mechanism of action, and then the greatest changes are seen to occur on the level of lipase and trypsin and the least -- on the level of the amylase activity and the bicarbonate alkalinity content. Note has also been taken of an age-specific statistically significant decline in the activity of the studied pancreatic enzymes in the blood (trypsin, its inhibitor, lipase, amylase) and in the urine (lipase, amylase).", "contents": "[State of the exocrine function of the pancreas during human aging]. A study of the exacrinous function of the pancreas made in 205 healthy persons aged from 18 to 90 has evidenced that in ageing there takes place in the duodenal mixture of juices a statistically significant decline in the activity of trypsin and of its inhibitor, lipase, amylase, in the level of bicarbonates and in the volume of the juice along with an increase in the content of chlorides. In ageing more marked changes become apparent following application of the agents stimulating the pancreatic secretion of the secretin and pancreozymin mechanism of action, and then the greatest changes are seen to occur on the level of lipase and trypsin and the least -- on the level of the amylase activity and the bicarbonate alkalinity content. Note has also been taken of an age-specific statistically significant decline in the activity of the studied pancreatic enzymes in the blood (trypsin, its inhibitor, lipase, amylase) and in the urine (lipase, amylase).", "PMID": 969354}
{"id": "pubmed23n0032_7520", "title": "[Nutrition and the state of health of the population of the UkrSSR].", "content": "Investigations made it possible to establish that in the period from 1925-28 to 1970-71 there took place in the Ukraine an increase in the consumption of animal products (milk, meat, fish, eggs), of sugar, vegetables, fruits and a fall in the consumption of cereals (bakery products, grits, macaroni, beans) and potato. Because of these dietary changes in the nutritional pattern of the population there were noted an elevated proportional share of proteins and fats, basically of animal origin, and a decrease in that of complex carbohydrates. A direct relation between the health status of the population and its alimentation has been ascertained; first--between the calorific value of the food products sets and their fat and carbohydrates content, on the one hand, and the body weight of the examined, on the other; and secondly--between the fluorine level in the food and the prevalence of fluorosis, caries, exostosis, aggravated by an imbalance of a number of nutritional components, on the other. Note has been taken at the same time of the fact that obese individuals suffer more often from cardio-vascular affections, gout, uro- and cholelithiasis and diabetes mellitus.", "contents": "[Nutrition and the state of health of the population of the UkrSSR]. Investigations made it possible to establish that in the period from 1925-28 to 1970-71 there took place in the Ukraine an increase in the consumption of animal products (milk, meat, fish, eggs), of sugar, vegetables, fruits and a fall in the consumption of cereals (bakery products, grits, macaroni, beans) and potato. Because of these dietary changes in the nutritional pattern of the population there were noted an elevated proportional share of proteins and fats, basically of animal origin, and a decrease in that of complex carbohydrates. A direct relation between the health status of the population and its alimentation has been ascertained; first--between the calorific value of the food products sets and their fat and carbohydrates content, on the one hand, and the body weight of the examined, on the other; and secondly--between the fluorine level in the food and the prevalence of fluorosis, caries, exostosis, aggravated by an imbalance of a number of nutritional components, on the other. Note has been taken at the same time of the fact that obese individuals suffer more often from cardio-vascular affections, gout, uro- and cholelithiasis and diabetes mellitus.", "PMID": 969355}
{"id": "pubmed23n0032_7521", "title": "[Effect of calorie deficient nutrition on thiol group content of liver cell nuclei from white rats of different ages].", "content": "The sulfydryl and disulfide groups content in summary histones of the hepatic cell nuclei in the Wistar albino rats aged 1, 3, 12 and 24 months was studied by employing amperometric titration under normal conditions and under the action on the organism of calorifically substandard nutrition tending to significantly prolong the life-span in these animals. In intact animals the content of thiol groups in histones was found to reach the maximum on the 3d month of their life, to continue at this level during the first year and to decline in old (34 month old) animals. In the post-natal ontogenesis of the rats the ratio of sulfhydryl to disulfide groups in the histone proteins does not show any noticeable changes. With calorific deficiency this ratio increases in histones of old animals. The possible significance of the thiol groups balance in the histone proteins in regulating the genome activity during age-dependent development of the animals is discussed.", "contents": "[Effect of calorie deficient nutrition on thiol group content of liver cell nuclei from white rats of different ages]. The sulfydryl and disulfide groups content in summary histones of the hepatic cell nuclei in the Wistar albino rats aged 1, 3, 12 and 24 months was studied by employing amperometric titration under normal conditions and under the action on the organism of calorifically substandard nutrition tending to significantly prolong the life-span in these animals. In intact animals the content of thiol groups in histones was found to reach the maximum on the 3d month of their life, to continue at this level during the first year and to decline in old (34 month old) animals. In the post-natal ontogenesis of the rats the ratio of sulfhydryl to disulfide groups in the histone proteins does not show any noticeable changes. With calorific deficiency this ratio increases in histones of old animals. The possible significance of the thiol groups balance in the histone proteins in regulating the genome activity during age-dependent development of the animals is discussed.", "PMID": 969356}
{"id": "pubmed23n0032_7522", "title": "[Processes of 14C-labeled glucose and dietary fat assimilation and metabolism].", "content": "14C-labeled butter, beef and mutton fats, naturally obtained in farm animals through biosynthesis, as well as oleic acid and glucose, put out by the industry, were introduced to albino male-rats. The values of absorption coefficients for the said substances, the rates of absorption and oxidation in tissues, the proportion of compounds eliminated from the organism in 24 hours together with the air in the form of CO2 through the lungs and skin and together with excreta were determined experimentally. It is shown that the glucose metabolism proceeds much more intensively than does the fat metabolism. The rate of assimilation and elimination of different edible fats depends upon their composition (ratio between saturated and unsaturated fatty acids).", "contents": "[Processes of 14C-labeled glucose and dietary fat assimilation and metabolism]. 14C-labeled butter, beef and mutton fats, naturally obtained in farm animals through biosynthesis, as well as oleic acid and glucose, put out by the industry, were introduced to albino male-rats. The values of absorption coefficients for the said substances, the rates of absorption and oxidation in tissues, the proportion of compounds eliminated from the organism in 24 hours together with the air in the form of CO2 through the lungs and skin and together with excreta were determined experimentally. It is shown that the glucose metabolism proceeds much more intensively than does the fat metabolism. The rate of assimilation and elimination of different edible fats depends upon their composition (ratio between saturated and unsaturated fatty acids).", "PMID": 969357}
{"id": "pubmed23n0032_7523", "title": "[Nutrition and disease].", "content": "An essentially new concept of the effect of nutrition on the development and evolution of diseases has been advanced. At its root there lies the theory of metabolic current as a main path for maintaining the homeokinesis. In the aspect of this concept the pathogenesis of malnutrition diseases is considered as affections due to imbalanced alimentation at the cellular level. Changes in the biochemical status of the organism occurring under stressor effects and the part played by the nutrition factor in adaptive reactions of the organism in response to the stressor effects are analyzed. Investigations concerning the role of nutrition in the genesis of adiposity and atherosclerosis are summarized. The paper offers a summary of the material contained in numerous research works of the author and his collaborators, as well as information published in the world literature that leave no doubt as to the effectiveness of enzymological approaches and, in particular, the validity of the metabolis current concept, all this being essential for understanding the significance of alimentary factors in preventing and treatment of many diseases.", "contents": "[Nutrition and disease]. An essentially new concept of the effect of nutrition on the development and evolution of diseases has been advanced. At its root there lies the theory of metabolic current as a main path for maintaining the homeokinesis. In the aspect of this concept the pathogenesis of malnutrition diseases is considered as affections due to imbalanced alimentation at the cellular level. Changes in the biochemical status of the organism occurring under stressor effects and the part played by the nutrition factor in adaptive reactions of the organism in response to the stressor effects are analyzed. Investigations concerning the role of nutrition in the genesis of adiposity and atherosclerosis are summarized. The paper offers a summary of the material contained in numerous research works of the author and his collaborators, as well as information published in the world literature that leave no doubt as to the effectiveness of enzymological approaches and, in particular, the validity of the metabolis current concept, all this being essential for understanding the significance of alimentary factors in preventing and treatment of many diseases.", "PMID": 969358}
{"id": "pubmed23n0032_7524", "title": "[Possible route for thiamine participation in fatty acid synthesis].", "content": "The possibility of thiamine partaking in the synthesis of fatty acids through the functions unrelated to the catalytic properties of thiamine-diphosphate was studied. Rats kept on a fat-free ration devoid of thiamine were given thiamine of thiochrome with no vitaminic properties. The total fatty acids content in different tissues and incorporation therein of tagged acetate and pyruvate was determined, while the fatty acids composition of the liver was investigated by using gas chromatography. Thiamine and thiochrome produced a similar effect on a number of the study factors, i.e. they forced down the total acids level in the spleen, intensified incorporation of tagged acetate and pyruvate in fatty acids of the heart and uniformly changed the fatty acids composition in the liver. It is suggested that the unindirectional effects of thiamine and thiochrome is due to the oxidative transformation of thiamine into thiochrome.", "contents": "[Possible route for thiamine participation in fatty acid synthesis]. The possibility of thiamine partaking in the synthesis of fatty acids through the functions unrelated to the catalytic properties of thiamine-diphosphate was studied. Rats kept on a fat-free ration devoid of thiamine were given thiamine of thiochrome with no vitaminic properties. The total fatty acids content in different tissues and incorporation therein of tagged acetate and pyruvate was determined, while the fatty acids composition of the liver was investigated by using gas chromatography. Thiamine and thiochrome produced a similar effect on a number of the study factors, i.e. they forced down the total acids level in the spleen, intensified incorporation of tagged acetate and pyruvate in fatty acids of the heart and uniformly changed the fatty acids composition in the liver. It is suggested that the unindirectional effects of thiamine and thiochrome is due to the oxidative transformation of thiamine into thiochrome.", "PMID": 969359}
{"id": "pubmed23n0032_7525", "title": "[Influence of the concentration and composition of dietary fats on biochemical changes in the liver and blood of rats during protracted muscular activity].", "content": "Tests made on adult albino rats kept for two weeks on isocaloric rations with an equal content of protein, but differing in that of fat and dissimilar in the fatty acids composition and the methionine level evidenced that in the event of a prolonged muscular activity the authors find rations with a not too high fat content (30 and less per cent of the aggregate caloricity), but sufficiently rich in linolic acid and methionine more adequate. Failure to comply with these conditions causes an inferior utilization of lipids during muscular activity and slowing down of the restitution processes at the time of rest. The character of the ration has no material effect on the mobilization of carbohydrates and lipids during muscular activity.", "contents": "[Influence of the concentration and composition of dietary fats on biochemical changes in the liver and blood of rats during protracted muscular activity]. Tests made on adult albino rats kept for two weeks on isocaloric rations with an equal content of protein, but differing in that of fat and dissimilar in the fatty acids composition and the methionine level evidenced that in the event of a prolonged muscular activity the authors find rations with a not too high fat content (30 and less per cent of the aggregate caloricity), but sufficiently rich in linolic acid and methionine more adequate. Failure to comply with these conditions causes an inferior utilization of lipids during muscular activity and slowing down of the restitution processes at the time of rest. The character of the ration has no material effect on the mobilization of carbohydrates and lipids during muscular activity.", "PMID": 969362}
{"id": "pubmed23n0032_7526", "title": "[Effect of different levels of protein and cellulose in the diet on the radiosensitivity of guinea pigs].", "content": "Investigations included 6 groups of male guinea pigs counting 50 guinea pigs in each of them. Starting from the age of 25 days the animals were kept on standard rations consisting of granulated combined fodder with diverse protein (22.8, 17.5, 13.8 per cent) and cellular tissue (11.8, 13.6, 15.7 per cent) content. The results failed to show any significant dependance of the animals' radiosensitivity on the protein and cellular tissue levels in the diet.", "contents": "[Effect of different levels of protein and cellulose in the diet on the radiosensitivity of guinea pigs]. Investigations included 6 groups of male guinea pigs counting 50 guinea pigs in each of them. Starting from the age of 25 days the animals were kept on standard rations consisting of granulated combined fodder with diverse protein (22.8, 17.5, 13.8 per cent) and cellular tissue (11.8, 13.6, 15.7 per cent) content. The results failed to show any significant dependance of the animals' radiosensitivity on the protein and cellular tissue levels in the diet.", "PMID": 969364}
{"id": "pubmed23n0032_7527", "title": "[Effect of diet therapy on several metabolic disorders in obese patients of different ages].", "content": "Data derived from a comparative study of some lipids and water metabolism rates in patients suffering mainly from metabolic-alimentary form of adiposity in 2 age groups (from 16 to 25 and from 35 years and upwards, respectively) are offered. In patients of both groups substantial changes in the lipid fractions of the blood were noted. The proportions of glycerides, beta-lipoproteins and free fatty acids in the blood were markedly increased. At the same time, all the patients, irrespective of their age, demonstrated a fall of the glucose-6-phosphate-dehydrogenase activity of the fatty tissue along with a reduced diurnal excretion of catecholamines with DOPA. There were in evidence characteristic disorders of the water metabolism that found their expression in an increased overall amount of water and also in a higher content of extra- and intracellular fluid. A low-calories diet used in conjunction with graded therapeutic exercises helped achieve a positive dynamics in the reduction of the patients' body weight along with a tendency toward normalization of the study metabolic rates in both of these age groups.", "contents": "[Effect of diet therapy on several metabolic disorders in obese patients of different ages]. Data derived from a comparative study of some lipids and water metabolism rates in patients suffering mainly from metabolic-alimentary form of adiposity in 2 age groups (from 16 to 25 and from 35 years and upwards, respectively) are offered. In patients of both groups substantial changes in the lipid fractions of the blood were noted. The proportions of glycerides, beta-lipoproteins and free fatty acids in the blood were markedly increased. At the same time, all the patients, irrespective of their age, demonstrated a fall of the glucose-6-phosphate-dehydrogenase activity of the fatty tissue along with a reduced diurnal excretion of catecholamines with DOPA. There were in evidence characteristic disorders of the water metabolism that found their expression in an increased overall amount of water and also in a higher content of extra- and intracellular fluid. A low-calories diet used in conjunction with graded therapeutic exercises helped achieve a positive dynamics in the reduction of the patients' body weight along with a tendency toward normalization of the study metabolic rates in both of these age groups.", "PMID": 969368}
{"id": "pubmed23n0032_7528", "title": "[Diet therapy of patients with chronic diffuse glomerulonephritis].", "content": "Results of dietotherapy used in dealing with 68 patients suffering from chronic diffuse glomerulonephritis at the stage of chronic renal incompetence and with 50 others with the nephrotic syndrome are reported. The pertinent observations and investigations indicate that the most adequate ration for patients with chronic renal insufficiency is the one containing 40 g of protein. With a far advanced renal incompetence the treatment is to be started with a diet containing 20 g of protein and then, as the condition of the patients gets better, puts them on a diet with 40 g of protein. Observations have also brought out the fact that patients with nephrotic syndrome are in need of a diet containing 1.5-2 g of protein per 1 kg of an edema-free body weight of the patient.", "contents": "[Diet therapy of patients with chronic diffuse glomerulonephritis]. Results of dietotherapy used in dealing with 68 patients suffering from chronic diffuse glomerulonephritis at the stage of chronic renal incompetence and with 50 others with the nephrotic syndrome are reported. The pertinent observations and investigations indicate that the most adequate ration for patients with chronic renal insufficiency is the one containing 40 g of protein. With a far advanced renal incompetence the treatment is to be started with a diet containing 20 g of protein and then, as the condition of the patients gets better, puts them on a diet with 40 g of protein. Observations have also brought out the fact that patients with nephrotic syndrome are in need of a diet containing 1.5-2 g of protein per 1 kg of an edema-free body weight of the patient.", "PMID": 969366}
{"id": "pubmed23n0032_7529", "title": "[Effect of a diet containing protein-mineral enrichment on the course of chronic gastritis with secretory inadequacy and ulcer desease].", "content": "The effect of a rational diet and of antiulcerous diets containing a proteinic-mineral enrichment (in an amount of 20--30 g) on the clinical symptomatology, the peripheral blood picture and some biochemical blood serum characteristics in patients with atrophic gastritis and duodenal ulcer was studied. In cases of atrophic gastritis with hypoferremia the proteinic enrichment was incorporated in the diet (rational) in an amount of 80--90 g per day. Control observations were conducted over analogous groups of patients receiving the same diets, but without proteinic enrichment. These observations continued over a period of 30--40 days. Thanks to a high content of easily assimilated iron and a well-balanced amino acids composition the proteinic-mineral enrichment has been found to be a valuable food product that is capable of favourably influencing the hemopoietic function of the organism and improving the blood proteins composition. Most patients with atrophic gastritis and peptic ulcer tolerate well the products and meals carrying additions of the proteinic enrichment.", "contents": "[Effect of a diet containing protein-mineral enrichment on the course of chronic gastritis with secretory inadequacy and ulcer desease]. The effect of a rational diet and of antiulcerous diets containing a proteinic-mineral enrichment (in an amount of 20--30 g) on the clinical symptomatology, the peripheral blood picture and some biochemical blood serum characteristics in patients with atrophic gastritis and duodenal ulcer was studied. In cases of atrophic gastritis with hypoferremia the proteinic enrichment was incorporated in the diet (rational) in an amount of 80--90 g per day. Control observations were conducted over analogous groups of patients receiving the same diets, but without proteinic enrichment. These observations continued over a period of 30--40 days. Thanks to a high content of easily assimilated iron and a well-balanced amino acids composition the proteinic-mineral enrichment has been found to be a valuable food product that is capable of favourably influencing the hemopoietic function of the organism and improving the blood proteins composition. Most patients with atrophic gastritis and peptic ulcer tolerate well the products and meals carrying additions of the proteinic enrichment.", "PMID": 969367}
{"id": "pubmed23n0032_7530", "title": "[Concentration of mercuro-organic compounds (methyl- and ethyl derivatives) in several species of fish and sea products].", "content": "By using gas-liquid chromatography the content of organic mercury compounds (methyl- and ethyl derivatives) in some widely consumed in alimentation fish species and marine products was determined. Methyl mercury was found in 96.2 per cent of a total of 104 samples. The average level of its content in the fish species under study was dissimilar, viz. in cod fish--0.045 + .0053 mg/kg. in sea perch--0.021 +/- 0.0029 mg/kg; in sprat--0.0050 +/- 0.0013 mg/kg. In marine products (crustacea, sea crayfish, shrimps) the percentage of the methyl mercury detection comprised 100, 60 and 85, respectively and the contamination level did not exceed 0.0520 mg/kg. Ethyl mercury was found in 33--35 per cent of 40 samples of shrimps and crustacea and in 13.6 per cent of fish subjected to analysis. From the above data the amount of methyl mercury introduced with a diurnal portion of fish was estamated at 0.9--5.0 psi per man-day.", "contents": "[Concentration of mercuro-organic compounds (methyl- and ethyl derivatives) in several species of fish and sea products]. By using gas-liquid chromatography the content of organic mercury compounds (methyl- and ethyl derivatives) in some widely consumed in alimentation fish species and marine products was determined. Methyl mercury was found in 96.2 per cent of a total of 104 samples. The average level of its content in the fish species under study was dissimilar, viz. in cod fish--0.045 + .0053 mg/kg. in sea perch--0.021 +/- 0.0029 mg/kg; in sprat--0.0050 +/- 0.0013 mg/kg. In marine products (crustacea, sea crayfish, shrimps) the percentage of the methyl mercury detection comprised 100, 60 and 85, respectively and the contamination level did not exceed 0.0520 mg/kg. Ethyl mercury was found in 33--35 per cent of 40 samples of shrimps and crustacea and in 13.6 per cent of fish subjected to analysis. From the above data the amount of methyl mercury introduced with a diurnal portion of fish was estamated at 0.9--5.0 psi per man-day.", "PMID": 969372}
{"id": "pubmed23n0032_7531", "title": "[Amino acid composition and biologic value of the proteins of several sorts of buckwheat].", "content": "The amino acids composition of summary proteins in unground buckwheat of four common and promising varieties grown in the Ukraine was investigated by using ion-exchange chromatography with an automatic analyzor Hd-1200 E. Between individual varieties of buckweheat no essential differences in the amino acids content were in evidence. The total proteins of the buckwheat grit contain high quantities of lysine, treonine, leucine, glutamic acid and arginine. The amino acids score was instrumental in determining the biological value and in eliciting amino acids limiting this value in different grits. These data may be made use of in the practice of public catering for estimating formulae of meals prepared with grits differently combined with other products securing an improved amino acids composition of ready-to-eat meals.", "contents": "[Amino acid composition and biologic value of the proteins of several sorts of buckwheat]. The amino acids composition of summary proteins in unground buckwheat of four common and promising varieties grown in the Ukraine was investigated by using ion-exchange chromatography with an automatic analyzor Hd-1200 E. Between individual varieties of buckweheat no essential differences in the amino acids content were in evidence. The total proteins of the buckwheat grit contain high quantities of lysine, treonine, leucine, glutamic acid and arginine. The amino acids score was instrumental in determining the biological value and in eliciting amino acids limiting this value in different grits. These data may be made use of in the practice of public catering for estimating formulae of meals prepared with grits differently combined with other products securing an improved amino acids composition of ready-to-eat meals.", "PMID": 969374}
{"id": "pubmed23n0032_7532", "title": "[Changes in the lipid component of the muscle tissue of several species of fish during refrigeration and heat treatment].", "content": "By using univariate thin-layer chromatography on silicagel IC254 of the company \"Chamapod Sz\u00e9choslovakia\" the qualitative and quantitative composition of the muscular tissue lipids in new oceanic fish--seriolella and epigonus was determined. It was ascertained that the qualitative composition of lipids changed during cold storage but insignificantly, due to hydrolysis of triglycerides and oxidation of phospholipids. In the course of heat treatment there occurs disintegration of the lipid-proteinic complexes attended by an accretion of phospholipids. An increment of free fatty acids is caused by the hydrolytic decomposition of triglycerides.", "contents": "[Changes in the lipid component of the muscle tissue of several species of fish during refrigeration and heat treatment]. By using univariate thin-layer chromatography on silicagel IC254 of the company \"Chamapod Sz\u00e9choslovakia\" the qualitative and quantitative composition of the muscular tissue lipids in new oceanic fish--seriolella and epigonus was determined. It was ascertained that the qualitative composition of lipids changed during cold storage but insignificantly, due to hydrolysis of triglycerides and oxidation of phospholipids. In the course of heat treatment there occurs disintegration of the lipid-proteinic complexes attended by an accretion of phospholipids. An increment of free fatty acids is caused by the hydrolytic decomposition of triglycerides.", "PMID": 969376}
{"id": "pubmed23n0032_7533", "title": "[Effect of qualitatively different proteins in the diet of patients with alimentary obesity on the course of the disease].", "content": "Among the population of the Ukrainian SSR 22 per cent of persons suffering from adiposity were revealed. Their nutritional pattern is characterized by an elevated caloritic value, a higher consumption of sugar, animal fats, as well as non-optimal structure of proteinic products. Of interest was elaboration and scientific substantiation of a dietetic nutrition in alimenary adiposity with due regard for the particularities specific for the alimentary pattern prevalent among the residents of the Ukrainian SSR. Under observation were kept 150 patients with alimentary obesity. The patients were kept on a low-calories diet (1450-1600 kcal) with restricted ingestion of carbohydrates (80--120 g), with physiological content of fat and protein. The 1st group of patients received meat and fish as a source of protein, the patients of the 2nd group were given a diet enriched with 100 g of the \"Ocean\" paste and those of the 3d group received a diet enriched with 150 g of egg whites. The greatest weight reduction was recorded in patients of the 3d group (10.96 +/- 0.48 kg), while in patients of the second (9.4 +/- 0.17 kg) and the first (8.5 +/- 0.34 kg) the reduction proved less marked. A diet enriched with egg white produced most beneficial effect also on the hormonal-metabolic disorders, viz. it helped to more significantly improve the adrenal function, that of the insular system, the blood serum protein metabolism rates, as well as those of the blood serum fat metabolism. The above investigations justify enrichment of the reducing diet with egg white and with the \"Okean\" paste.", "contents": "[Effect of qualitatively different proteins in the diet of patients with alimentary obesity on the course of the disease]. Among the population of the Ukrainian SSR 22 per cent of persons suffering from adiposity were revealed. Their nutritional pattern is characterized by an elevated caloritic value, a higher consumption of sugar, animal fats, as well as non-optimal structure of proteinic products. Of interest was elaboration and scientific substantiation of a dietetic nutrition in alimenary adiposity with due regard for the particularities specific for the alimentary pattern prevalent among the residents of the Ukrainian SSR. Under observation were kept 150 patients with alimentary obesity. The patients were kept on a low-calories diet (1450-1600 kcal) with restricted ingestion of carbohydrates (80--120 g), with physiological content of fat and protein. The 1st group of patients received meat and fish as a source of protein, the patients of the 2nd group were given a diet enriched with 100 g of the \"Ocean\" paste and those of the 3d group received a diet enriched with 150 g of egg whites. The greatest weight reduction was recorded in patients of the 3d group (10.96 +/- 0.48 kg), while in patients of the second (9.4 +/- 0.17 kg) and the first (8.5 +/- 0.34 kg) the reduction proved less marked. A diet enriched with egg white produced most beneficial effect also on the hormonal-metabolic disorders, viz. it helped to more significantly improve the adrenal function, that of the insular system, the blood serum protein metabolism rates, as well as those of the blood serum fat metabolism. The above investigations justify enrichment of the reducing diet with egg white and with the \"Okean\" paste.", "PMID": 969370}
{"id": "pubmed23n0032_7534", "title": "[Determination of residual amounts of antibiotics of the tetracycline series in meat products].", "content": "An analysis of literature sources and the authors' own experimental investigations served as a basis for the proposed method of determining residual amounts of the tetracycline series antibiotics in meat products. The method provides for the use of the test-culture Bac. cereus ATCC 11778, nutrient medium on the basis of Hottinger's hydrolysate and of a citrate-hydrochloride extracting mixture.", "contents": "[Determination of residual amounts of antibiotics of the tetracycline series in meat products]. An analysis of literature sources and the authors' own experimental investigations served as a basis for the proposed method of determining residual amounts of the tetracycline series antibiotics in meat products. The method provides for the use of the test-culture Bac. cereus ATCC 11778, nutrient medium on the basis of Hottinger's hydrolysate and of a citrate-hydrochloride extracting mixture.", "PMID": 969377}
{"id": "pubmed23n0032_7535", "title": "[Effect of long-term vitamin A administration on the acid fastness and biochemical properties of erythrocytes].", "content": "A long-term administration of retinol in a dose exceeding 15-fold the diurnal requirement to rats weighing 170-200 g provoked a diminution of the erythrocytes resistance to an acid hemolytic, an intensified uptake of glucose, and increased activity of glycolytic enzymes (hexokinase, aldolase, phosphohexoisomerase), accumulation of lactate, along with changes in the redox enzymes activity, suppression of the catalase and intensification of peroxidase activity. The content of microergic nucleotides and electrolites (Na+ and K+) remained unchanged.", "contents": "[Effect of long-term vitamin A administration on the acid fastness and biochemical properties of erythrocytes]. A long-term administration of retinol in a dose exceeding 15-fold the diurnal requirement to rats weighing 170-200 g provoked a diminution of the erythrocytes resistance to an acid hemolytic, an intensified uptake of glucose, and increased activity of glycolytic enzymes (hexokinase, aldolase, phosphohexoisomerase), accumulation of lactate, along with changes in the redox enzymes activity, suppression of the catalase and intensification of peroxidase activity. The content of microergic nucleotides and electrolites (Na+ and K+) remained unchanged.", "PMID": 969379}
{"id": "pubmed23n0032_7536", "title": "[Effect of the rate of heating ground meat products with ultra-high frequency energy on completing their preparation].", "content": "The influence of the heating rate on the readiness of forced meat products determined from inactivation of the acid phosphatase and the temperature of the product, was studied. Changes in the enzyme activity and in organoleptic characteristics were studied by using forced meat prepared from high-quality beef (2 per cent of kitchen salt, curing in a salt solution for 24 hours at 4 degrees with addition of 25 per cent of water to the raw meat during cutting). The analyses were made when the temperature of the forced meat reached 68-80 degrees (with an interval of 1 degree) the rates of heating being as follows: 0, 1, 1, 2, 4, 6, 8, 10 degrees with the field tension ranging from 2000 up to 16000 V/m. The power applied amounted to 40-2000 W. The readiness of a product undergoing the UHF-treatment with different heating rates was found to be achieved at temperatures ranging from 72.0 to 75.2 degrees and it may be assessed objectively from inactivation of the acid phosphatase and with reference to the temperature of the product matching each heating rate individually. An equation from which the temperature of the forced meat products readiness may be determined is given.", "contents": "[Effect of the rate of heating ground meat products with ultra-high frequency energy on completing their preparation]. The influence of the heating rate on the readiness of forced meat products determined from inactivation of the acid phosphatase and the temperature of the product, was studied. Changes in the enzyme activity and in organoleptic characteristics were studied by using forced meat prepared from high-quality beef (2 per cent of kitchen salt, curing in a salt solution for 24 hours at 4 degrees with addition of 25 per cent of water to the raw meat during cutting). The analyses were made when the temperature of the forced meat reached 68-80 degrees (with an interval of 1 degree) the rates of heating being as follows: 0, 1, 1, 2, 4, 6, 8, 10 degrees with the field tension ranging from 2000 up to 16000 V/m. The power applied amounted to 40-2000 W. The readiness of a product undergoing the UHF-treatment with different heating rates was found to be achieved at temperatures ranging from 72.0 to 75.2 degrees and it may be assessed objectively from inactivation of the acid phosphatase and with reference to the temperature of the product matching each heating rate individually. An equation from which the temperature of the forced meat products readiness may be determined is given.", "PMID": 969380}
{"id": "pubmed23n0032_7537", "title": "[Role of incomplete dietary protein in experimental formation of gallstones].", "content": "The influence exerted by the quality of alimentary protein on the composition of the secreted bile, morphology of the liver and gallbladder, as well as on the formation of gallstones was studied in experiments with rats and hamsters. It is shown that with inclusion in an experimental fat-free ration of qualitatively incomplete protein gelatin (instead of caseine) the frequency of the gallstones formation increases by almost 6 times. In animals this is attended by a reduced concentration of a number of major bile components. Upon feeding for 2 weeks on lithogenic ration with gelatin morphological investigations disclose inflammatory changes in the gallbladder wall, and after a lapse of 3 weeks--multiple exulcerations in the mucosa and a dense gross sediment in the gallbladder lumen.", "contents": "[Role of incomplete dietary protein in experimental formation of gallstones]. The influence exerted by the quality of alimentary protein on the composition of the secreted bile, morphology of the liver and gallbladder, as well as on the formation of gallstones was studied in experiments with rats and hamsters. It is shown that with inclusion in an experimental fat-free ration of qualitatively incomplete protein gelatin (instead of caseine) the frequency of the gallstones formation increases by almost 6 times. In animals this is attended by a reduced concentration of a number of major bile components. Upon feeding for 2 weeks on lithogenic ration with gelatin morphological investigations disclose inflammatory changes in the gallbladder wall, and after a lapse of 3 weeks--multiple exulcerations in the mucosa and a dense gross sediment in the gallbladder lumen.", "PMID": 969378}
{"id": "pubmed23n0032_7538", "title": "[Extractability of lipids from fish and fish products].", "content": "The completness of the lipids removal from the fish muscles and fish products was investigated by making extraction in a filtering separating funnel (FSF) formerly proposed for determining lipids in oil-bearing seeds and cereals. The new modified procedure was contrasted against the standard one (accepted by the State Standards) used in separating and quantitative determination of the lipids content in fish by using the Socklet apparatus for extraction. The proposed variant of extraction was found to secure a higher yield of the lipids. The pattern of analysis used in determining the lipids content in vegetable products may well be used also in investigating animal products, such as, fish and fish products.", "contents": "[Extractability of lipids from fish and fish products]. The completness of the lipids removal from the fish muscles and fish products was investigated by making extraction in a filtering separating funnel (FSF) formerly proposed for determining lipids in oil-bearing seeds and cereals. The new modified procedure was contrasted against the standard one (accepted by the State Standards) used in separating and quantitative determination of the lipids content in fish by using the Socklet apparatus for extraction. The proposed variant of extraction was found to secure a higher yield of the lipids. The pattern of analysis used in determining the lipids content in vegetable products may well be used also in investigating animal products, such as, fish and fish products.", "PMID": 969381}
{"id": "pubmed23n0032_7539", "title": "Expression of genetic markers of erythrocyte immunoglobulin G autoantibodies in autoimmune hemolytic anemia.", "content": "The Gm allotype constitution of the autoantibody molecules and the serum Gm phenotypes were determined in 19 patients with autiommune hemolytic anemia of IgG type. The results showed that the known heterogeneity of the antierythrocyte autoantibodies in this disease concerns not only the immunoglobulin class and the isotypic subclass, but also the genetic markers of these molecules. Evidence was obtained that the anomalous production of antierythrocyte autoantibodies is polyclonal, that thse autoantibodies belong mainly to the IgG1 isotypic subclass, and that they have a preference for the Gm(1) allotype. In addition, certain of the findings point to a preferential expression of the Gm(1,21) haplotype.", "contents": "Expression of genetic markers of erythrocyte immunoglobulin G autoantibodies in autoimmune hemolytic anemia. The Gm allotype constitution of the autoantibody molecules and the serum Gm phenotypes were determined in 19 patients with autiommune hemolytic anemia of IgG type. The results showed that the known heterogeneity of the antierythrocyte autoantibodies in this disease concerns not only the immunoglobulin class and the isotypic subclass, but also the genetic markers of these molecules. Evidence was obtained that the anomalous production of antierythrocyte autoantibodies is polyclonal, that thse autoantibodies belong mainly to the IgG1 isotypic subclass, and that they have a preference for the Gm(1) allotype. In addition, certain of the findings point to a preferential expression of the Gm(1,21) haplotype.", "PMID": 969390}
{"id": "pubmed23n0032_7540", "title": "The HLA polymorphism and susceptibility to disease.", "content": "Remarkable differences were observed when 1,465 healthy Caucasian individuals and 128 healthy Negro individuals were compared for the genetic distribution of 25 different HLA antigens. Caucasians had a significantly higher frequency of A1, A3, B8, and Bw16, and Negroes of A28 and Aw30. The haplotype which had the highest incidence as well as the greatest positive linkage disequilibrium was A1-B8 among Caucasians and A2-B12 among Negroes. Genetic distance between the two races was 0.0592. The 89 Caucasian patients with renal failure did not demonstrate any significant deviations in phenotype frequencies (PF) of various antigens, when compared with healthy Caucasians; however, 48 similar Negro patients had twice as high an incidence of Bw17 as the healthy Negroes. No significant deviation in PF was observed in 77 Caucasian patients who had leukemia; however, 32 Caucasian patients who had back pain (24 also had back stiffness) due to spondylitic, arthritic or disc syndrome, had a significant increase of Bw16 and of B27, and a decrease of B12, when compared to Caucasian controls.", "contents": "The HLA polymorphism and susceptibility to disease. Remarkable differences were observed when 1,465 healthy Caucasian individuals and 128 healthy Negro individuals were compared for the genetic distribution of 25 different HLA antigens. Caucasians had a significantly higher frequency of A1, A3, B8, and Bw16, and Negroes of A28 and Aw30. The haplotype which had the highest incidence as well as the greatest positive linkage disequilibrium was A1-B8 among Caucasians and A2-B12 among Negroes. Genetic distance between the two races was 0.0592. The 89 Caucasian patients with renal failure did not demonstrate any significant deviations in phenotype frequencies (PF) of various antigens, when compared with healthy Caucasians; however, 48 similar Negro patients had twice as high an incidence of Bw17 as the healthy Negroes. No significant deviation in PF was observed in 77 Caucasian patients who had leukemia; however, 32 Caucasian patients who had back pain (24 also had back stiffness) due to spondylitic, arthritic or disc syndrome, had a significant increase of Bw16 and of B27, and a decrease of B12, when compared to Caucasian controls.", "PMID": 969389}
{"id": "pubmed23n0032_7541", "title": "Gas chromatographic trace analysis of beta-propiolactone in sterilized serum proteins.", "content": "A method for detecting traces of beta-propiolactone, which was previously described by Schmitz-Masse, has been modified and adapted to the analysis of protein solutions. Using this method, the rate of hydrolysis of beta-propiolactone (beta-P1) was examined and beta-P1 sterilized commercial products were analyzed for possible residual nonhydrolyzed beta-P1.", "contents": "Gas chromatographic trace analysis of beta-propiolactone in sterilized serum proteins. A method for detecting traces of beta-propiolactone, which was previously described by Schmitz-Masse, has been modified and adapted to the analysis of protein solutions. Using this method, the rate of hydrolysis of beta-propiolactone (beta-P1) was examined and beta-P1 sterilized commercial products were analyzed for possible residual nonhydrolyzed beta-P1.", "PMID": 969391}
{"id": "pubmed23n0032_7542", "title": "Variations of H, A and B antigen strengths among Melanesians.", "content": "Melanesians of groups O, A1 and B all show greater variation of H antigen strength than do Caucasians. This is least marked in O bloods. Although the average H content of Melanesian and Caucasian samples did not differ significantly, 10% of O and over 20% of A1 and B bloods from coastal Austronesian speakers had higher H than was found among Caucasians, There is evidence that some non-Austronesian populations in New Guinea have twice this incidence of A1 high H. Melanesian and Bantu H, A1 and B genes appear to function similarly, in that their B genes act more effectively than Caucasian, their A1 genes less so, while H produces greater variation. It is suggested that this gene interaction, rather than the significantly greater availability of H or substrate in the Bantu, causes the observed variations from the Caucasian pattern found in the other races. Subjects with selective antigenic depression were excluded from the comparative studies, but the relevance of this phenomenon to apparent H content in Melanesians is discussed.", "contents": "Variations of H, A and B antigen strengths among Melanesians. Melanesians of groups O, A1 and B all show greater variation of H antigen strength than do Caucasians. This is least marked in O bloods. Although the average H content of Melanesian and Caucasian samples did not differ significantly, 10% of O and over 20% of A1 and B bloods from coastal Austronesian speakers had higher H than was found among Caucasians, There is evidence that some non-Austronesian populations in New Guinea have twice this incidence of A1 high H. Melanesian and Bantu H, A1 and B genes appear to function similarly, in that their B genes act more effectively than Caucasian, their A1 genes less so, while H produces greater variation. It is suggested that this gene interaction, rather than the significantly greater availability of H or substrate in the Bantu, causes the observed variations from the Caucasian pattern found in the other races. Subjects with selective antigenic depression were excluded from the comparative studies, but the relevance of this phenomenon to apparent H content in Melanesians is discussed.", "PMID": 969392}
{"id": "pubmed23n0032_7543", "title": "Hepatitis B antibody detection in solid-phase radioimmunoassay.", "content": "A simple radioimmunologic procedure for the detection of anti-hepatitis B antibody using the 125I Ausria kit (Abbott) is described. The rapidity, sensitivity and high specificity of this method are underlined.", "contents": "Hepatitis B antibody detection in solid-phase radioimmunoassay. A simple radioimmunologic procedure for the detection of anti-hepatitis B antibody using the 125I Ausria kit (Abbott) is described. The rapidity, sensitivity and high specificity of this method are underlined.", "PMID": 969393}
{"id": "pubmed23n0032_7544", "title": "[Modeling calculations of pesticide contamination of horticultural products].", "content": "The growth of the pesticies production and of the scope of their use can produce in man and in his environment some adverse consequences, the elimination of which is possibly by their proper and safe management. In order to solve this problem it is necessary to elicit basic factors determining this process and also to foresee the outcome of it under changing conditions, i.e. three successive stages are involved, viz. modelling-forecasting-management. In the event of complex multiparametric natural phenomena, among which stands also the process of interaction between pesticides and various environmental objects, the realization of the said stages invariably presupposes the use of mathematical approaches, and one of these is considered in the present work.", "contents": "[Modeling calculations of pesticide contamination of horticultural products]. The growth of the pesticies production and of the scope of their use can produce in man and in his environment some adverse consequences, the elimination of which is possibly by their proper and safe management. In order to solve this problem it is necessary to elicit basic factors determining this process and also to foresee the outcome of it under changing conditions, i.e. three successive stages are involved, viz. modelling-forecasting-management. In the event of complex multiparametric natural phenomena, among which stands also the process of interaction between pesticides and various environmental objects, the realization of the said stages invariably presupposes the use of mathematical approaches, and one of these is considered in the present work.", "PMID": 969383}
{"id": "pubmed23n0032_7545", "title": "[Radioisotopic studies in cancer of the thyroid gland].", "content": "The authors analyze the results from the carried out radioisotope investigations in preoperation and postoperation period of 42 patients with thyroid gland cancer. The test for 131J cumulation and thyreoscintigraphy were performed prior to operation. A part of the patients was stressed to reveal hyperthyroid function of the thyroid gland. The cases of cold nodules of the thyroid gland prevail on the scintigraphy. The test for 131J cumulation, thyreoscintigraphy, determination of the level of the proteinbound radioiodine in serum and Hamolski test were carried out as control investigations after the operation prior to percutaneous radiation and radioiodine therapy. Patients with hypothyroidism predominate in the first investigation. The cases with partial resection of thyroid gland prevail in scintigraphy, with cases of \"white\" scintigraphy and cases with normal scintigraphyic image of the gland. The proteinbound radioiodine in serum, besides the majority of the cases with normal values, in a part of the examined-shows etevated values. Homolski test in the majority of the patients is within the normal limits and the cases with elevated vales might be due to the specific changes in the capapcity-binding ability of thyroxinbinding glubin, but are always suspicious for metastases in function. The complex radioisotope investigation of the thyroid gland are basic methods for the early thyroid cancer diagnosis, for the determination of the therapeutic trends and prognosis of the patient and for the follow-up in dynamics the progress of the disease and the effect of the carried out treatment.", "contents": "[Radioisotopic studies in cancer of the thyroid gland]. The authors analyze the results from the carried out radioisotope investigations in preoperation and postoperation period of 42 patients with thyroid gland cancer. The test for 131J cumulation and thyreoscintigraphy were performed prior to operation. A part of the patients was stressed to reveal hyperthyroid function of the thyroid gland. The cases of cold nodules of the thyroid gland prevail on the scintigraphy. The test for 131J cumulation, thyreoscintigraphy, determination of the level of the proteinbound radioiodine in serum and Hamolski test were carried out as control investigations after the operation prior to percutaneous radiation and radioiodine therapy. Patients with hypothyroidism predominate in the first investigation. The cases with partial resection of thyroid gland prevail in scintigraphy, with cases of \"white\" scintigraphy and cases with normal scintigraphyic image of the gland. The proteinbound radioiodine in serum, besides the majority of the cases with normal values, in a part of the examined-shows etevated values. Homolski test in the majority of the patients is within the normal limits and the cases with elevated vales might be due to the specific changes in the capapcity-binding ability of thyroxinbinding glubin, but are always suspicious for metastases in function. The complex radioisotope investigation of the thyroid gland are basic methods for the early thyroid cancer diagnosis, for the determination of the therapeutic trends and prognosis of the patient and for the follow-up in dynamics the progress of the disease and the effect of the carried out treatment.", "PMID": 969473}
{"id": "pubmed23n0032_7546", "title": "[Character of the rheumatic heart lesions in the current treatment and prevention of rheumatism].", "content": "An analysis and assessment of the character of heart lesions, their severity, development and influence by the contemporary complex antirheumatic treatment is performed on 1147 rheumatic patients. It was concluded that due to the organized struggle and prophylaxis of rheumatism as well as the timely diagnosis and early and complex antirheumatic treatment with the inclusion of relatively high doses of corticohormones, the rheumatic attacks develop lightly with a predominant affect of myocardium but to a light degree.", "contents": "[Character of the rheumatic heart lesions in the current treatment and prevention of rheumatism]. An analysis and assessment of the character of heart lesions, their severity, development and influence by the contemporary complex antirheumatic treatment is performed on 1147 rheumatic patients. It was concluded that due to the organized struggle and prophylaxis of rheumatism as well as the timely diagnosis and early and complex antirheumatic treatment with the inclusion of relatively high doses of corticohormones, the rheumatic attacks develop lightly with a predominant affect of myocardium but to a light degree.", "PMID": 969475}
{"id": "pubmed23n0032_7547", "title": "[Unilateral bronchography at a young age and ventilatory function].", "content": "In 21 males, aged about 20, 18 out of them with chronic bronchopulmonary diseases (bronchiectases, focal fibrosis, deforming bronchitis) and three healthy--VC, FEFR1, FVC, MMV50, MEFR200-1200, MAEFR, MAEFR25-75 and MEFR50 and MEFR90 were spirographically investigated prior to, two hours afert and 24 hours after unilateral bronchography; the three of the investigations were combined with a subsequent inhalation bronchodilatation test with orziprenalin--aersol (alupent). A mixed (restrictive-obstructive) ventilation syndrome with bronchospasm was established to develop after the bronchography. The restrictive syndrome is prevailing and that is conditioned by the absence of a definite manifestation of the obstruction at the level of the flow rates with small pulmonary volmes. The restrictive syndrome is admitted to be conditioned by \"fear of coughing and the obstructuve-by the opaque medium and bronchospasm after the injection of the  opaque substance.", "contents": "[Unilateral bronchography at a young age and ventilatory function]. In 21 males, aged about 20, 18 out of them with chronic bronchopulmonary diseases (bronchiectases, focal fibrosis, deforming bronchitis) and three healthy--VC, FEFR1, FVC, MMV50, MEFR200-1200, MAEFR, MAEFR25-75 and MEFR50 and MEFR90 were spirographically investigated prior to, two hours afert and 24 hours after unilateral bronchography; the three of the investigations were combined with a subsequent inhalation bronchodilatation test with orziprenalin--aersol (alupent). A mixed (restrictive-obstructive) ventilation syndrome with bronchospasm was established to develop after the bronchography. The restrictive syndrome is prevailing and that is conditioned by the absence of a definite manifestation of the obstruction at the level of the flow rates with small pulmonary volmes. The restrictive syndrome is admitted to be conditioned by \"fear of coughing and the obstructuve-by the opaque medium and bronchospasm after the injection of the  opaque substance.", "PMID": 969477}
{"id": "pubmed23n0032_7548", "title": "[Obstructive changes in the lung of young smokers].", "content": "The results from the spirometric investigations of pulmonary function in comparable  grpis pf 28 smokers and 37 non-smokers at the age of 20 are presented. Positive obstructive changes were established to be present in the lungs of the smokers (smoked about 20 cigarettes daily in the course of four years and more); they were varified by the essential decrease of FEO1, MRFR200-1200, MAEFR25-75, EFR25 and EFR10. MMV50 is decreased with a critical statistical significance.", "contents": "[Obstructive changes in the lung of young smokers]. The results from the spirometric investigations of pulmonary function in comparable  grpis pf 28 smokers and 37 non-smokers at the age of 20 are presented. Positive obstructive changes were established to be present in the lungs of the smokers (smoked about 20 cigarettes daily in the course of four years and more); they were varified by the essential decrease of FEO1, MRFR200-1200, MAEFR25-75, EFR25 and EFR10. MMV50 is decreased with a critical statistical significance.", "PMID": 969478}
{"id": "pubmed23n0032_7549", "title": "[Urine specific gravity and osmolarity in hypertension patients with dehydration].", "content": "Results are reported from the comparative investigations of kidney concentration function by urine specific gravity and its osmolatity of 58 patients with arterial hypertension. Significant differences were found in more of the half of the patients. On the base of certain theoretical prerequisites and possibilities of technical errors was concluded that the determination of the maximal urine osmolality is a more reliable and more accurate index for the actual renal concentration ability. Urine specific gravity can be used in the everyday clinical practice due to the more convenient and easier determination. Urine osmolality must be used predominantly in scientific-clinical studies.", "contents": "[Urine specific gravity and osmolarity in hypertension patients with dehydration]. Results are reported from the comparative investigations of kidney concentration function by urine specific gravity and its osmolatity of 58 patients with arterial hypertension. Significant differences were found in more of the half of the patients. On the base of certain theoretical prerequisites and possibilities of technical errors was concluded that the determination of the maximal urine osmolality is a more reliable and more accurate index for the actual renal concentration ability. Urine specific gravity can be used in the everyday clinical practice due to the more convenient and easier determination. Urine osmolality must be used predominantly in scientific-clinical studies.", "PMID": 969479}
{"id": "pubmed23n0032_7550", "title": "[Cortisol secretion rate (CSR) in hypertension and endocrine patients].", "content": "With the method of isotope dilution the circadian cortisol secretion was determined in 22 patients with hypertonic disease--28.35 mg/24 h, elevated versus CSR in healthy subjects-16.63 mg/24 h. CSR is elevated also in 5 patients with bilateral adenocortical hyperplasia and with suprarenal cancer. The patients with diencephalic syndrome, progressing according to the type of Cushing disease have elevated absolute values of CSR, but recalculated per square meter of body surface and per gram creatineine excreted in 24 h-CSR proved to be normal-about 12 mg/24 h.", "contents": "[Cortisol secretion rate (CSR) in hypertension and endocrine patients]. With the method of isotope dilution the circadian cortisol secretion was determined in 22 patients with hypertonic disease--28.35 mg/24 h, elevated versus CSR in healthy subjects-16.63 mg/24 h. CSR is elevated also in 5 patients with bilateral adenocortical hyperplasia and with suprarenal cancer. The patients with diencephalic syndrome, progressing according to the type of Cushing disease have elevated absolute values of CSR, but recalculated per square meter of body surface and per gram creatineine excreted in 24 h-CSR proved to be normal-about 12 mg/24 h.", "PMID": 969480}
{"id": "pubmed23n0032_7551", "title": "[Morphological changes in the gastric and duodenal mucosa in duodenal ulcer in young persons].", "content": "Histological investigations were carried out upon the state of the gastric and duodena mucosa in 27 young patients with duodenal ulcer, seven out of them being in the state of exaverbation of the ulcer and 20-in remission. While the duodenal mucosa in the majority of the cases (88.9%) was established to be affected, in 85.2 percent of the patients the gastric mucosa remained intact. The chronic duodenitis observe n 24 of the cases have no atrophic character. The changes in the mucous secretion of duodenal mucosa are in direct correlation with the state of exacerbation or remission of the ulcer process. Similar correlation was not established in gastric mucosa.", "contents": "[Morphological changes in the gastric and duodenal mucosa in duodenal ulcer in young persons]. Histological investigations were carried out upon the state of the gastric and duodena mucosa in 27 young patients with duodenal ulcer, seven out of them being in the state of exaverbation of the ulcer and 20-in remission. While the duodenal mucosa in the majority of the cases (88.9%) was established to be affected, in 85.2 percent of the patients the gastric mucosa remained intact. The chronic duodenitis observe n 24 of the cases have no atrophic character. The changes in the mucous secretion of duodenal mucosa are in direct correlation with the state of exacerbation or remission of the ulcer process. Similar correlation was not established in gastric mucosa.", "PMID": 969481}
{"id": "pubmed23n0032_7552", "title": "[Serum haptoglobin and sialic acid content and the spontaneous rosette test values as indices of the activity of the disease process in chronic glomerulonephritis].", "content": "The diagnostic significance of haptoglobin is studied as well as of sialic acid and spontaneous rosette test (SRT) and ESR as indices of the morbid process activity in 74 patients with chronic hephritis with manifested and oligospymptomatic course and in a stage of therapeutic remission and in 14 patients with acute protracted nephritis. The parallelism of the changes in all parameters studied is proved in acute protracted nephritis and in chronic nephritis with manifested course. In patients with oligosymtomatically advancing chronic nephritis, in spite of the normal values of ESR and the rest of the routine indices for determination of the morbid process activity, haptoglobin, sialic acid and SRT were established to be increased. They were hardly normalized after the advancement of a lasting therpeutic remission. That all gives grounds to the authors to think, that the significance of the studied indices for the assessment of the morbid process activity in oligosymptomatically advancing chronic glomerulonephritis, is superior, to a certain extent, to the diagnostic signficance of ESR. Their follow up in dynamics permits their application in the determination of the immunosuppressive treatment efficiency.", "contents": "[Serum haptoglobin and sialic acid content and the spontaneous rosette test values as indices of the activity of the disease process in chronic glomerulonephritis]. The diagnostic significance of haptoglobin is studied as well as of sialic acid and spontaneous rosette test (SRT) and ESR as indices of the morbid process activity in 74 patients with chronic hephritis with manifested and oligospymptomatic course and in a stage of therapeutic remission and in 14 patients with acute protracted nephritis. The parallelism of the changes in all parameters studied is proved in acute protracted nephritis and in chronic nephritis with manifested course. In patients with oligosymtomatically advancing chronic nephritis, in spite of the normal values of ESR and the rest of the routine indices for determination of the morbid process activity, haptoglobin, sialic acid and SRT were established to be increased. They were hardly normalized after the advancement of a lasting therpeutic remission. That all gives grounds to the authors to think, that the significance of the studied indices for the assessment of the morbid process activity in oligosymptomatically advancing chronic glomerulonephritis, is superior, to a certain extent, to the diagnostic signficance of ESR. Their follow up in dynamics permits their application in the determination of the immunosuppressive treatment efficiency.", "PMID": 969482}
{"id": "pubmed23n0032_7553", "title": "[Determination of the tonus of the rectosigmoid flexure with a new design of rectosigmoid tonometer].", "content": "An apparatus is constructed for the determination of the rectosigmoid transition tonus-rectosigmoid tonometer. There pressure will be read in Torr. The investigation of the rectosigmoid tonus of 75 healthy subjects established that it ranges between 15-30 Torr. Pathologically changed RST was established in 37-elevated in 12 patients and reduced in 21 and zero in 4-during the tonus determination of 100 patients with different forms of chronic colitis. The results from the rectosigmoid tonometry must be interpreted together with the rest of the indices (AT, size of rectal folds, etc).", "contents": "[Determination of the tonus of the rectosigmoid flexure with a new design of rectosigmoid tonometer]. An apparatus is constructed for the determination of the rectosigmoid transition tonus-rectosigmoid tonometer. There pressure will be read in Torr. The investigation of the rectosigmoid tonus of 75 healthy subjects established that it ranges between 15-30 Torr. Pathologically changed RST was established in 37-elevated in 12 patients and reduced in 21 and zero in 4-during the tonus determination of 100 patients with different forms of chronic colitis. The results from the rectosigmoid tonometry must be interpreted together with the rest of the indices (AT, size of rectal folds, etc).", "PMID": 969483}
{"id": "pubmed23n0032_7554", "title": "Spirometry in the evaluation of pulmonary function.", "content": "Spirometry should be more widely used in routine examinations. Equipment should meet the individual physician's or hospital's needs and include either a dependable water-sealed spirometer or an easily calibrated and accurate electronic spirometer. Justifiable concern over the reliability of electronic spirometers has resulted in requests to determine performance standards for these medical devices. Predicted normal standards must apply to the particular spirometer. Recommended tests are those of vital capacity (VC), forced vital capacity (FVC), one-second forced expiratory volume (FEV(1)), the ratio of one-second forced expiratory flow (FEF200-1200) and forced midexpiratory flow (FEF25-75 percent). The maximum voluntary ventilation (MVV) test may be useful for evaluation of work disability and detection of extrathoracic obstruction. Additional consideration may be given to measurements of total lung capacity (TLC) to discriminate between restrictive and obstructive impairment and the forced end-expiratory flow (FEF75-85 percent) to detect mild small airway obstruction. At this time, flow-volume curves measurement cannot be justified for routine clinical use.", "contents": "Spirometry in the evaluation of pulmonary function. Spirometry should be more widely used in routine examinations. Equipment should meet the individual physician's or hospital's needs and include either a dependable water-sealed spirometer or an easily calibrated and accurate electronic spirometer. Justifiable concern over the reliability of electronic spirometers has resulted in requests to determine performance standards for these medical devices. Predicted normal standards must apply to the particular spirometer. Recommended tests are those of vital capacity (VC), forced vital capacity (FVC), one-second forced expiratory volume (FEV(1)), the ratio of one-second forced expiratory flow (FEF200-1200) and forced midexpiratory flow (FEF25-75 percent). The maximum voluntary ventilation (MVV) test may be useful for evaluation of work disability and detection of extrathoracic obstruction. Additional consideration may be given to measurements of total lung capacity (TLC) to discriminate between restrictive and obstructive impairment and the forced end-expiratory flow (FEF75-85 percent) to detect mild small airway obstruction. At this time, flow-volume curves measurement cannot be justified for routine clinical use.", "PMID": 969495}
{"id": "pubmed23n0032_7555", "title": "Role of platelets in hemostasis and thrombosis.", "content": "Platelets interact with the coagulation factors in a complex way to arrest bleeding or generate thrombi. Recently, the platelet's relationship to endothelial alteration and atheroma production has received renewed attention. At present, tests of platelet function better define \"hypocoagulable\" rather than \"hypercoagulable\" states.", "contents": "Role of platelets in hemostasis and thrombosis. Platelets interact with the coagulation factors in a complex way to arrest bleeding or generate thrombi. Recently, the platelet's relationship to endothelial alteration and atheroma production has received renewed attention. At present, tests of platelet function better define \"hypocoagulable\" rather than \"hypercoagulable\" states.", "PMID": 969503}
{"id": "pubmed23n0032_7556", "title": "Heparin prophylaxis for deep vein thrombosis and pulmonary embolism.", "content": "Thromboembolic prophylaxis remains a significant problem and is obviously incompletely understood. It would appear, however, that at present the information available implies several points. First, administration of low-dose heparin is efficacious in preventing deep vein thrombosis and pulmonary emboli in most general surgical patients who are at risk for thromboembolic complications. Second, low-dose heparin probably works by augmenting the effect of the naturally occurring inhibitor to Factor Xa. Third, patients in whom surgical operations are done and extensive tissue dissection or postoperative immobilization (such as hip arthroplasties) is required are probably not protected by low-dose heparin administration; full anticoagulation with warfarin or treatment with one of the platelet antiaggregating agents should be carried out. Fourth, any form of anticoagulation carries some risk of bleeding complications, but it appears that the incidence of major bleeding complications is not significantly greater in the treatment groups.", "contents": "Heparin prophylaxis for deep vein thrombosis and pulmonary embolism. Thromboembolic prophylaxis remains a significant problem and is obviously incompletely understood. It would appear, however, that at present the information available implies several points. First, administration of low-dose heparin is efficacious in preventing deep vein thrombosis and pulmonary emboli in most general surgical patients who are at risk for thromboembolic complications. Second, low-dose heparin probably works by augmenting the effect of the naturally occurring inhibitor to Factor Xa. Third, patients in whom surgical operations are done and extensive tissue dissection or postoperative immobilization (such as hip arthroplasties) is required are probably not protected by low-dose heparin administration; full anticoagulation with warfarin or treatment with one of the platelet antiaggregating agents should be carried out. Fourth, any form of anticoagulation carries some risk of bleeding complications, but it appears that the incidence of major bleeding complications is not significantly greater in the treatment groups.", "PMID": 969504}
{"id": "pubmed23n0032_7557", "title": "Detection and management of deep vein thrombosis.", "content": "The accuracy of diagnosis of deep venous thrombosis is significantly improved by combining clinical evaluation with other adjunctive methods, especially fibrinogen uptake tests, technetium scans, Doppler techniques and phlebography. Using these studies, early treatment with intravenous administration of heparin can be begun and in selected cases with long-term risks, warfarin is often useful. These same drugs, in different dosage schedules, may also be helpful as prophylaxis. With these methods of treatment, thrombectomy and caval interruption are required less often. If interruption of inferior vena cava flow becomes necessary, several new methods utilizing intracaval filters are proving to be very useful.", "contents": "Detection and management of deep vein thrombosis. The accuracy of diagnosis of deep venous thrombosis is significantly improved by combining clinical evaluation with other adjunctive methods, especially fibrinogen uptake tests, technetium scans, Doppler techniques and phlebography. Using these studies, early treatment with intravenous administration of heparin can be begun and in selected cases with long-term risks, warfarin is often useful. These same drugs, in different dosage schedules, may also be helpful as prophylaxis. With these methods of treatment, thrombectomy and caval interruption are required less often. If interruption of inferior vena cava flow becomes necessary, several new methods utilizing intracaval filters are proving to be very useful.", "PMID": 969505}
{"id": "pubmed23n0032_7558", "title": "Arbitration agreements and your malpractice coverage.", "content": "With new legislation and favorable case law developments, many providers of health care services are turning to binding arbitration agreements as a partial solution to the medical malpractice crisis. Existing data indicate tremendous advantages can be gained from the use of such agreements, if they are carefully drawn to comply with new law, tailored to the particular situation in which they will be used and coupled with appropriate procedures to secure the intended benefits. Arbitration is not a substitute for insurance, but if a provider has taken the calculated risk of forgoing insurance he should not be without a carefully drawn binding arbitration agreement.", "contents": "Arbitration agreements and your malpractice coverage. With new legislation and favorable case law developments, many providers of health care services are turning to binding arbitration agreements as a partial solution to the medical malpractice crisis. Existing data indicate tremendous advantages can be gained from the use of such agreements, if they are carefully drawn to comply with new law, tailored to the particular situation in which they will be used and coupled with appropriate procedures to secure the intended benefits. Arbitration is not a substitute for insurance, but if a provider has taken the calculated risk of forgoing insurance he should not be without a carefully drawn binding arbitration agreement.", "PMID": 969512}
{"id": "pubmed23n0032_7559", "title": "[Acute coronary surgery].", "content": "While the indications for surgical treatment of chronic angina pectoris are exactly defined, the delineation between conservative and surgical therapy of acute complications of coronary diseases is not standardized. The application of possible surgical procedures to acute coronary surgery (aorto-coronary bypass, infarctectomy, correction of post-infarction ventricular septal defects and mitral insufficiency) are discussed in relation to the following clinical pictures: 1. Unstable angina 2. Surgery after myocardial infarction a) recent infarction b) cardiogenic shock after infarction c) impending reinfarction d) post-infarction tachyarrhythmia unresponsive to conservative and electrical therapy e) acute heart failure caused by post-infarction defect of the ventricular septum or by mitral insufficiency. The following therapeutic guide-lines can be laid down on the basis of an analysis of experience gained in this field by other workers and ourselves: surgical therapy is indicated in the cases of unstable angina, impending reinfarction, or acute heart failure caused by a post-infarction ventricular septal defect or by mitral insufficiency which do not respond rapidly to conservatives measures. By contrast, the superiority of surgical intervention, even with the assistance of intraaortic balloon pumping, has not yet been established for the treatment of the remainder of the above-mentioned clinical pictures and requires evaluation by further investigations.", "contents": "[Acute coronary surgery]. While the indications for surgical treatment of chronic angina pectoris are exactly defined, the delineation between conservative and surgical therapy of acute complications of coronary diseases is not standardized. The application of possible surgical procedures to acute coronary surgery (aorto-coronary bypass, infarctectomy, correction of post-infarction ventricular septal defects and mitral insufficiency) are discussed in relation to the following clinical pictures: 1. Unstable angina 2. Surgery after myocardial infarction a) recent infarction b) cardiogenic shock after infarction c) impending reinfarction d) post-infarction tachyarrhythmia unresponsive to conservative and electrical therapy e) acute heart failure caused by post-infarction defect of the ventricular septum or by mitral insufficiency. The following therapeutic guide-lines can be laid down on the basis of an analysis of experience gained in this field by other workers and ourselves: surgical therapy is indicated in the cases of unstable angina, impending reinfarction, or acute heart failure caused by a post-infarction ventricular septal defect or by mitral insufficiency which do not respond rapidly to conservatives measures. By contrast, the superiority of surgical intervention, even with the assistance of intraaortic balloon pumping, has not yet been established for the treatment of the remainder of the above-mentioned clinical pictures and requires evaluation by further investigations.", "PMID": 969524}
{"id": "pubmed23n0032_7560", "title": "[Surgical complications in pacemaker operations and their management].", "content": "A review is presented of the range of surgical complications encountered in 120 out of 749 patients (16%) receiving a pacemaker implantation over the period October 1974 to September 1975. These complications can be subdivided into those involving interruption or disturbance of the conduction of the pacemaker impulse and those associated with impaired wound-healing. The aetiology, the diagnosis and the surgical management are discussed.", "contents": "[Surgical complications in pacemaker operations and their management]. A review is presented of the range of surgical complications encountered in 120 out of 749 patients (16%) receiving a pacemaker implantation over the period October 1974 to September 1975. These complications can be subdivided into those involving interruption or disturbance of the conduction of the pacemaker impulse and those associated with impaired wound-healing. The aetiology, the diagnosis and the surgical management are discussed.", "PMID": 969525}
{"id": "pubmed23n0032_7561", "title": "[Early operation in toxic megacolon complicating ulcerative colitis].", "content": "A case report of toxic megacolon complicating ulcerative colitis serves as basis for a discussion of the diagnosis, therapeutic management and assessment of the indications for surgical interference in this condition. In this particular case, colectomy with retention of the rectal stump proved a life-saving measure. The rectal stump had to be removed three months subsequently in view of the persistence of the mucosal pathology.", "contents": "[Early operation in toxic megacolon complicating ulcerative colitis]. A case report of toxic megacolon complicating ulcerative colitis serves as basis for a discussion of the diagnosis, therapeutic management and assessment of the indications for surgical interference in this condition. In this particular case, colectomy with retention of the rectal stump proved a life-saving measure. The rectal stump had to be removed three months subsequently in view of the persistence of the mucosal pathology.", "PMID": 969526}
{"id": "pubmed23n0032_7562", "title": "[Continuous telemetric recording of intracranial pressure].", "content": "A report is given on the development of a telemetric system for continuous recording of intracranial pressure, using an external energy source. The pressure transducer, called Teleceptor, is placed in the subdural space through a burr hole. The resonance frequencies of the transmitter and receiver circuits in the implanted transducer are influenced by a pressure-sensitive membrane in such a way that pressure variations result in resonance point displacement and energy transfer reduction. The necessary tuning of the transmitter frequency to maximum energy transfer is in direct proportion to the magnitude of pressure modification.", "contents": "[Continuous telemetric recording of intracranial pressure]. A report is given on the development of a telemetric system for continuous recording of intracranial pressure, using an external energy source. The pressure transducer, called Teleceptor, is placed in the subdural space through a burr hole. The resonance frequencies of the transmitter and receiver circuits in the implanted transducer are influenced by a pressure-sensitive membrane in such a way that pressure variations result in resonance point displacement and energy transfer reduction. The necessary tuning of the transmitter frequency to maximum energy transfer is in direct proportion to the magnitude of pressure modification.", "PMID": 969527}
{"id": "pubmed23n0032_7563", "title": "[Radiological assessment of diaphragmatic function in operated cases of congenital defects of the diaphragm].", "content": "An investigation was undertaken in a series of 10 children operated upon for congenital diaphragmatic defects in regard to the value of simple thoracic X-ray pictures for functional assessment of the repaired diaphragm. The radiographs were taken in forced inspiration as well as in forced expiration and indices were calculated for the mobility of the diaphragm and the costophrenic angle. Moreover, an attempt was made to estimate diaphragmatic contraction by comparing the length of the hemidiaphragm during inspiration and during expiration. The obtained results gave sufficient information for routine investigations of diaphragmatic function and compared well with those of radiocinescopy. An impairment of the mobility of the diaphragm and the costophrenic angle was statistically demonstrated in our follow-up series as compared with a control group of healthy children.", "contents": "[Radiological assessment of diaphragmatic function in operated cases of congenital defects of the diaphragm]. An investigation was undertaken in a series of 10 children operated upon for congenital diaphragmatic defects in regard to the value of simple thoracic X-ray pictures for functional assessment of the repaired diaphragm. The radiographs were taken in forced inspiration as well as in forced expiration and indices were calculated for the mobility of the diaphragm and the costophrenic angle. Moreover, an attempt was made to estimate diaphragmatic contraction by comparing the length of the hemidiaphragm during inspiration and during expiration. The obtained results gave sufficient information for routine investigations of diaphragmatic function and compared well with those of radiocinescopy. An impairment of the mobility of the diaphragm and the costophrenic angle was statistically demonstrated in our follow-up series as compared with a control group of healthy children.", "PMID": 969528}
{"id": "pubmed23n0032_7564", "title": "[Neurological urology -- a new specialty].", "content": "Neuro-urological methods for investigating neurogenic bladder dysfunctions are discussed. A knowledge of central and autonomous pathways of bladder innervation is the basis for an understanding of the pharmacodynamics of the bladder. Successful treatment of neurogenic bladder dysfunction is impossible without urodynamic assessment. Conservative, as well as operative measures of therapy are mentioned. Urinary diversion (ileal conduit) is nowadays no longer undertaken merely as a last resort to prevent chronic upper urinary tract infection, stone formation, contracted kidney and uremia, but is a positive, practicable alternative for the disabled patient. Especially for the younger patient, in whom the social aspects of this situation are of great importance, ileal conduit provides the possibility of a socially-adjusted existence.", "contents": "[Neurological urology -- a new specialty]. Neuro-urological methods for investigating neurogenic bladder dysfunctions are discussed. A knowledge of central and autonomous pathways of bladder innervation is the basis for an understanding of the pharmacodynamics of the bladder. Successful treatment of neurogenic bladder dysfunction is impossible without urodynamic assessment. Conservative, as well as operative measures of therapy are mentioned. Urinary diversion (ileal conduit) is nowadays no longer undertaken merely as a last resort to prevent chronic upper urinary tract infection, stone formation, contracted kidney and uremia, but is a positive, practicable alternative for the disabled patient. Especially for the younger patient, in whom the social aspects of this situation are of great importance, ileal conduit provides the possibility of a socially-adjusted existence.", "PMID": 969529}
{"id": "pubmed23n0032_7565", "title": "Metabolism of linoleamides. I. Absorption, excretion and metabolism of N-(alpha-methylbenzyl)linoleamide in rat and man.", "content": "1. In urine of rats dosed with N-(alpha-methylbenzyl)linoleamide (MBLA), three dicarboxylic acid monoamides, N-(alpha-methylbenzyl)succinic acid monoamide, N-(alpha-methylbenzyl)glutaric acid monoamide and N-(alpha-methylbenzyl)adipic acid monoamide, were identified. Conjugated alpha-methylbenzylamine, hippuric acid and conjugates of the dicarboxylic acid monoamides were also found in the urine. N-(alpha-Methylbenzyl)succinic acid monoamide was the main metabolite in rats. 2. Biliary excretion of radioactivity was studied in rats, cannulated for collection of bile and duodenal infusion, after oral administration of N-(alpha-methylbenzyl)[1-14C]linoleamide. With constant duodenal infusion of bile, about 7% of the dose was excreted in the bile, while excretion of radioactivity was negligible without bile infusion. 3. The g.l.c. analysis of human urine after oral administration of MBLA revealed that two dicarboxylic acid monoamides were present and N-(alpha-methylbenzyl)succinic acid monoamide was the main metabolite. 4. MBLA was excreted unchanged in the faeces of men who received MBLA to the extent of about 53% dose in 3 days. 5. MBLA was not detected (less than 1 mug/ml) in the serum of a volunteer who had been taking an oral daily dose of 1500 mg of MBLA for 3 months.", "contents": "Metabolism of linoleamides. I. Absorption, excretion and metabolism of N-(alpha-methylbenzyl)linoleamide in rat and man. 1. In urine of rats dosed with N-(alpha-methylbenzyl)linoleamide (MBLA), three dicarboxylic acid monoamides, N-(alpha-methylbenzyl)succinic acid monoamide, N-(alpha-methylbenzyl)glutaric acid monoamide and N-(alpha-methylbenzyl)adipic acid monoamide, were identified. Conjugated alpha-methylbenzylamine, hippuric acid and conjugates of the dicarboxylic acid monoamides were also found in the urine. N-(alpha-Methylbenzyl)succinic acid monoamide was the main metabolite in rats. 2. Biliary excretion of radioactivity was studied in rats, cannulated for collection of bile and duodenal infusion, after oral administration of N-(alpha-methylbenzyl)[1-14C]linoleamide. With constant duodenal infusion of bile, about 7% of the dose was excreted in the bile, while excretion of radioactivity was negligible without bile infusion. 3. The g.l.c. analysis of human urine after oral administration of MBLA revealed that two dicarboxylic acid monoamides were present and N-(alpha-methylbenzyl)succinic acid monoamide was the main metabolite. 4. MBLA was excreted unchanged in the faeces of men who received MBLA to the extent of about 53% dose in 3 days. 5. MBLA was not detected (less than 1 mug/ml) in the serum of a volunteer who had been taking an oral daily dose of 1500 mg of MBLA for 3 months.", "PMID": 969560}
{"id": "pubmed23n0032_7566", "title": "Metabolism of linoleamides. II. Absorption, distribution, excretion and metabolism of N-[alpha-phenyl-beta-(p-tolyl)ethyl]linoleamide.", "content": "1. Absorption, distribution, excretion and metabolism of (-)N-[alpha-phenyl-beta-(p-tolyl)ethyl][14C]linoleamide (14C-PTLA) were studied in rats and dogs. Faecal excretion of PTLA was studied in dogs and men by g.l.c. 2. 14C-PTLA (10 mg/kg) given orally to rats resulted in urinary and faecal excretion of radioactivity of 2 and 93%, respectively, by male rats and 8 and 87% by female rats in 48 h. Faecal excretion of PTLA in men was similar to that in rats. 3. Distribution of radioactivity in rats and dogs after oral administration of 14C-PTLA showed that a major part of the dose was not absorbed. 4. N-[alpha-phenyl-beta-(p-tolyl)ethyl]glutaric acid monoamide were detected in the urine of rats dosed orally with 14C-PTLA.", "contents": "Metabolism of linoleamides. II. Absorption, distribution, excretion and metabolism of N-[alpha-phenyl-beta-(p-tolyl)ethyl]linoleamide. 1. Absorption, distribution, excretion and metabolism of (-)N-[alpha-phenyl-beta-(p-tolyl)ethyl][14C]linoleamide (14C-PTLA) were studied in rats and dogs. Faecal excretion of PTLA was studied in dogs and men by g.l.c. 2. 14C-PTLA (10 mg/kg) given orally to rats resulted in urinary and faecal excretion of radioactivity of 2 and 93%, respectively, by male rats and 8 and 87% by female rats in 48 h. Faecal excretion of PTLA in men was similar to that in rats. 3. Distribution of radioactivity in rats and dogs after oral administration of 14C-PTLA showed that a major part of the dose was not absorbed. 4. N-[alpha-phenyl-beta-(p-tolyl)ethyl]glutaric acid monoamide were detected in the urine of rats dosed orally with 14C-PTLA.", "PMID": 969561}
{"id": "pubmed23n0032_7567", "title": "Urinary metabolites of p-hydroxyamphetamine in man, rat and guinea-pig.", "content": "1. The metabolism of (+/-)-p-hydroxy[14C]amphetamine has been studied in the rat, guinea-pig and man. 2. Most of the administered 14C was excreted in the urine within the first 24 h (64-92%), and was present mainly as free and conjugated p-hydroxy[14C]-amphetamine. In the female rat and female guinea-pig the conjugate was a glucuronide, but in man, who received a much smaller dose, the conjugate was a sulphate ester. A sex difference in conjugation was found in the rat, the female partly conjugating the drug but not the male. 3. Small quantities (1-6% of dose) of p-hydroxynorephedrine, a putative false neurotransmitter, were found in the urine of the three species. 4. Some oxidative degradation of the side chain of p-hydroxyamphetamine occurred in rat and guinea-pig since small amounts of p-hydroxybenzoic acid (1-3%) were detected in the urine.", "contents": "Urinary metabolites of p-hydroxyamphetamine in man, rat and guinea-pig. 1. The metabolism of (+/-)-p-hydroxy[14C]amphetamine has been studied in the rat, guinea-pig and man. 2. Most of the administered 14C was excreted in the urine within the first 24 h (64-92%), and was present mainly as free and conjugated p-hydroxy[14C]-amphetamine. In the female rat and female guinea-pig the conjugate was a glucuronide, but in man, who received a much smaller dose, the conjugate was a sulphate ester. A sex difference in conjugation was found in the rat, the female partly conjugating the drug but not the male. 3. Small quantities (1-6% of dose) of p-hydroxynorephedrine, a putative false neurotransmitter, were found in the urine of the three species. 4. Some oxidative degradation of the side chain of p-hydroxyamphetamine occurred in rat and guinea-pig since small amounts of p-hydroxybenzoic acid (1-3%) were detected in the urine.", "PMID": 969562}
{"id": "pubmed23n0032_7568", "title": "Peculiar pharmacokinetics of brallobarbital as a source of complications in Vesparax intoxication.", "content": "1. The rate of elimination of brallobarbital, a component of Vesparax depends on the route of administration. The half-life following oral administration is considerably longer than after intravenous injection. This corresponds with longer sleeping times. 2. The half-life of brallobarbital administered intravenously could be increased by administration of its metabolites. This suggests that a metabolite, formed especially after oral administration, is responsible for the retarded elimination of brallobarbital. 3. The presence of the other components of Vesparax, namely secobarbital and hydroxyzine also increases the half-life of brallobarbital. 4. Brallobarbital, rather than the other components of Vesparax is primarily responsible for the problems in Vesparax intoxication.", "contents": "Peculiar pharmacokinetics of brallobarbital as a source of complications in Vesparax intoxication. 1. The rate of elimination of brallobarbital, a component of Vesparax depends on the route of administration. The half-life following oral administration is considerably longer than after intravenous injection. This corresponds with longer sleeping times. 2. The half-life of brallobarbital administered intravenously could be increased by administration of its metabolites. This suggests that a metabolite, formed especially after oral administration, is responsible for the retarded elimination of brallobarbital. 3. The presence of the other components of Vesparax, namely secobarbital and hydroxyzine also increases the half-life of brallobarbital. 4. Brallobarbital, rather than the other components of Vesparax is primarily responsible for the problems in Vesparax intoxication.", "PMID": 969563}
{"id": "pubmed23n0032_7569", "title": "The binding of trichlorofluoromethane and other haloalkanes to cytochrome P-450 under aerobic and anaerobic conditions.", "content": "1. Trichlorofluoromethane and other haloalkanes interact with hepatic microsomal cytochrome P-450 to give type I spectra under aerobic conditions. 2. Anaerobically, CCl3F, CCl2F. CClF2 and CCl4 interact to give peaks at approx. 420 nm and 455 nm; the other haloalkanes gave peaks at approx. 420 nm only. The formation of the 452 nm peak for CCl3F was time-dependent, and dependent on substrate concentration. 3. Iso-octane extraction of the microsomal preparation increased the magnitude of spectral interaction under reducing conditions but not its rate of formation. Phenobarbitone pretreatment increased the magnitude of the spectral interaction; 3-methylcholanthrene pre-treatment had no effect on the magnitude of the 452 nm peak but decreased its rate of formation. 4. It is suggested that the 452 nm peak represents the interaction of a reactive species formed from CCl3F with the Fe2+ of the haem of reduced cytochrome P-450.", "contents": "The binding of trichlorofluoromethane and other haloalkanes to cytochrome P-450 under aerobic and anaerobic conditions. 1. Trichlorofluoromethane and other haloalkanes interact with hepatic microsomal cytochrome P-450 to give type I spectra under aerobic conditions. 2. Anaerobically, CCl3F, CCl2F. CClF2 and CCl4 interact to give peaks at approx. 420 nm and 455 nm; the other haloalkanes gave peaks at approx. 420 nm only. The formation of the 452 nm peak for CCl3F was time-dependent, and dependent on substrate concentration. 3. Iso-octane extraction of the microsomal preparation increased the magnitude of spectral interaction under reducing conditions but not its rate of formation. Phenobarbitone pretreatment increased the magnitude of the spectral interaction; 3-methylcholanthrene pre-treatment had no effect on the magnitude of the 452 nm peak but decreased its rate of formation. 4. It is suggested that the 452 nm peak represents the interaction of a reactive species formed from CCl3F with the Fe2+ of the haem of reduced cytochrome P-450.", "PMID": 969564}
{"id": "pubmed23n0032_7570", "title": "Studies on the metabolism of d-limonene (p-mentha-1,8-diene). IV. Isolation and characterization of new metabolites and species differences in metabolism.", "content": "1. The main route of elimination of d-limonene administered orally was via the urine in animals and man, 75-95% of the administered radioactivity being excreted in the urine during 2-3 days. Faecal excretion accounted for less than 10% of the dose in animals during 2-3 days. 2. In addition to six metabolites, namely p-mentha-1,8-dien-10-ol (M-I), p-menth-1-ene-8,9-diol (M-II), perillic acid (M-III), perillic acid-8,9-diol (M-IV), p-mentha-1,8-dien-10-yl-beta-D-glucopyranosiduronic acid (M-V) and 8-hydroxy-p-meth-1-en-9-yl-beta-D-glucopyranosiduronic acid (M-VI) isolated from rabbit urine previously (Kodama et al., 1974), five new metabolites have been isolated from dog and rat urine, and which were characterized as 2-hydroxy-p-menth-8-en-7-oic acid (M-VII), perillylglycine (M-VIII), perillyl-beta-D-glucopyranosiduronic acid (M-IX), p-mentha-1,8-dien-6-ol (M-X) and probably p-menth-1-ene-6,8,9-triol (M-XI). 3. The major metabolite of d-limonene in the urine was M-IV in rat and rabbit, M-IX in hamster, M-II in dog and M-VI in guinea pig and man.", "contents": "Studies on the metabolism of d-limonene (p-mentha-1,8-diene). IV. Isolation and characterization of new metabolites and species differences in metabolism. 1. The main route of elimination of d-limonene administered orally was via the urine in animals and man, 75-95% of the administered radioactivity being excreted in the urine during 2-3 days. Faecal excretion accounted for less than 10% of the dose in animals during 2-3 days. 2. In addition to six metabolites, namely p-mentha-1,8-dien-10-ol (M-I), p-menth-1-ene-8,9-diol (M-II), perillic acid (M-III), perillic acid-8,9-diol (M-IV), p-mentha-1,8-dien-10-yl-beta-D-glucopyranosiduronic acid (M-V) and 8-hydroxy-p-meth-1-en-9-yl-beta-D-glucopyranosiduronic acid (M-VI) isolated from rabbit urine previously (Kodama et al., 1974), five new metabolites have been isolated from dog and rat urine, and which were characterized as 2-hydroxy-p-menth-8-en-7-oic acid (M-VII), perillylglycine (M-VIII), perillyl-beta-D-glucopyranosiduronic acid (M-IX), p-mentha-1,8-dien-6-ol (M-X) and probably p-menth-1-ene-6,8,9-triol (M-XI). 3. The major metabolite of d-limonene in the urine was M-IV in rat and rabbit, M-IX in hamster, M-II in dog and M-VI in guinea pig and man.", "PMID": 969565}
{"id": "pubmed23n0032_7571", "title": "Characterization of a red pigment-antibiotic produced by Streptomyces sp. strain NRC-C7.", "content": "The streptomycete strain NRC-C 7 isolated from some Egyptian soil samples, produces a red-coloured pH indicator antibiotic active against gram positive bacteria. The producing organism belongs to the grey series of the genus Streptomyces and has grey coloured aerial mycelium with violet pigmented substrate mycelium, the violet pigment does not diffuse into the agar medium. Glucose sodium nitrate medium proved the most suitable medium for the production of this antibiotic. The pigment was extracted from the broth and purified. The solubility, Rf values, minimal inhibiting concentrations, optical rotation, UV, and IR spectra were studied. Melting point 180, elemental analysis: carbon 51.71%, hydrogen 6.25%, and oxygen 42.04%.", "contents": "Characterization of a red pigment-antibiotic produced by Streptomyces sp. strain NRC-C7. The streptomycete strain NRC-C 7 isolated from some Egyptian soil samples, produces a red-coloured pH indicator antibiotic active against gram positive bacteria. The producing organism belongs to the grey series of the genus Streptomyces and has grey coloured aerial mycelium with violet pigmented substrate mycelium, the violet pigment does not diffuse into the agar medium. Glucose sodium nitrate medium proved the most suitable medium for the production of this antibiotic. The pigment was extracted from the broth and purified. The solubility, Rf values, minimal inhibiting concentrations, optical rotation, UV, and IR spectra were studied. Melting point 180, elemental analysis: carbon 51.71%, hydrogen 6.25%, and oxygen 42.04%.", "PMID": 969566}
{"id": "pubmed23n0032_7572", "title": "[Protein crystals and tubuli bundles in yeast cells. V. Enrichment of crystal and tubuli proteins during O2 limitation and anaerobiosis].", "content": "Cells of Saccharomyces carlsbergensis contain proteins assembling to crystals and bundles of tubules (tb) in the cyto- and karyoplasm by osmotic shock with hypertonic solutions. The cellular concentration of these proteins is regulated by oxygen pressure during growth. In cells grown at optimal aeration the protein level is low and crystals and tb cannot be induced. After a short period of O2-limitation or anerobic growth conditions the protein concentration increases and induction of crystals and tb is possible.", "contents": "[Protein crystals and tubuli bundles in yeast cells. V. Enrichment of crystal and tubuli proteins during O2 limitation and anaerobiosis]. Cells of Saccharomyces carlsbergensis contain proteins assembling to crystals and bundles of tubules (tb) in the cyto- and karyoplasm by osmotic shock with hypertonic solutions. The cellular concentration of these proteins is regulated by oxygen pressure during growth. In cells grown at optimal aeration the protein level is low and crystals and tb cannot be induced. After a short period of O2-limitation or anerobic growth conditions the protein concentration increases and induction of crystals and tb is possible.", "PMID": 969567}
{"id": "pubmed23n0032_7573", "title": "Effects of 3'-fluorothymidine, nalidixic acid, and mitomycin C on temperature-sensitive mutants of the DNA polymerase of phage T4.", "content": "The temperature-sensitive DNA polymerase mutator mutants A58 and  L98 are less inhibited by 3'-Fluorothymidine than the T4 wild type and the antimutator mutant CB121, in consequence of the assumed differences between the polymerase-associated exonuclease activities. This interpretation is confirmed by results with nalidixic acid and mitomycin C. The use of systems of different temperature-sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) studies on the mechanism of enzyme action and the functions affected in temperature-sensitive enzymes, and 3.) for enzyme-specific drug screening.", "contents": "Effects of 3'-fluorothymidine, nalidixic acid, and mitomycin C on temperature-sensitive mutants of the DNA polymerase of phage T4. The temperature-sensitive DNA polymerase mutator mutants A58 and  L98 are less inhibited by 3'-Fluorothymidine than the T4 wild type and the antimutator mutant CB121, in consequence of the assumed differences between the polymerase-associated exonuclease activities. This interpretation is confirmed by results with nalidixic acid and mitomycin C. The use of systems of different temperature-sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) studies on the mechanism of enzyme action and the functions affected in temperature-sensitive enzymes, and 3.) for enzyme-specific drug screening.", "PMID": 969568}
{"id": "pubmed23n0032_7574", "title": "Inhibition of acetate and propionate formation upon aeration of resting cells of the anaerobic Propionibacterium shermanii: evidence of the Pasteur reaction.", "content": "When resting cell suspensions of the anaerobic P. shermanii were brought to an oxygen concentration of 0.64 mumoles/ml, acid formation was completely inhibited. The cells started to respire on the propionic acid previously accumulated furing anaerobiosis. Glucose consumption was concomitantly decreased to about 60 percent of the rate during anaerobiosis. As the viability of the cells was not affected by the transition to aerobic conditions, the changes observed upon aeration were ascribed to the regulatory properties of the Pasteur reaction. Damage inflicted by oxygen was encountered in the rapid inactivation of propionate respiration. This damage outlived the time of oxygenation, and was manifested during the subsequent anaerobiosis in the decreased activity to form propionate. This indicates that oxygen may inactivate one (or more) enzyme(s) involved in the metabolism of propionate. The viability of cells in buffer, and glucose-containing buffer, was found to be only insignificantly decreased by oxygen in the range from 0 to 500 mumoles of oxygen per g of wet cells.", "contents": "Inhibition of acetate and propionate formation upon aeration of resting cells of the anaerobic Propionibacterium shermanii: evidence of the Pasteur reaction. When resting cell suspensions of the anaerobic P. shermanii were brought to an oxygen concentration of 0.64 mumoles/ml, acid formation was completely inhibited. The cells started to respire on the propionic acid previously accumulated furing anaerobiosis. Glucose consumption was concomitantly decreased to about 60 percent of the rate during anaerobiosis. As the viability of the cells was not affected by the transition to aerobic conditions, the changes observed upon aeration were ascribed to the regulatory properties of the Pasteur reaction. Damage inflicted by oxygen was encountered in the rapid inactivation of propionate respiration. This damage outlived the time of oxygenation, and was manifested during the subsequent anaerobiosis in the decreased activity to form propionate. This indicates that oxygen may inactivate one (or more) enzyme(s) involved in the metabolism of propionate. The viability of cells in buffer, and glucose-containing buffer, was found to be only insignificantly decreased by oxygen in the range from 0 to 500 mumoles of oxygen per g of wet cells.", "PMID": 969569}
{"id": "pubmed23n0032_7575", "title": "Keratin decomposition by dermatophytes. II. Presence of s-sulfocysteine and cysteic acid in soluble decomposition products.", "content": "The dermatophyte Microsporum gypseum was grown on human hair in a mineral medium. Filtrates of cultures of various age were chromatographed on Sephadex gels and further characterized by chemical analysis and thin-layer chromatography. The products of keratin decomposition were separated into two fractions. The first fraction formed 10 to 20% of the bulk and contained proteins low in sulfur content. The second, very wide fraction contained oligopeptides and polypeptides with molecular weights from several hundreds up to approximately 13,000 with a peak at 1,000-2,000 daltons. This fraction contained a relatively high amount of cystine, S-sulfocysteine, and cysteic acid. S-sulfocysteine was present in the free state and also combined in peptides where its amount exceeded that of cystine. This supports the author's hypothesis on keratin decomposition by dermatophytes. According to this hypothesis, the fungus excretes sulfite which cleaves the disulfide bonds of keratin to cysteine and S-sulfocysteine. The substrate, denatured by sulfitolysis, is then more easily digestible by fungal proteases. Besides S-sulfocysteine, comparable amounts of cysteic acid were found in all analyses. This compound most probably originated by air oxidation of sulfur amino acids in the alkaline cultivation fluid.", "contents": "Keratin decomposition by dermatophytes. II. Presence of s-sulfocysteine and cysteic acid in soluble decomposition products. The dermatophyte Microsporum gypseum was grown on human hair in a mineral medium. Filtrates of cultures of various age were chromatographed on Sephadex gels and further characterized by chemical analysis and thin-layer chromatography. The products of keratin decomposition were separated into two fractions. The first fraction formed 10 to 20% of the bulk and contained proteins low in sulfur content. The second, very wide fraction contained oligopeptides and polypeptides with molecular weights from several hundreds up to approximately 13,000 with a peak at 1,000-2,000 daltons. This fraction contained a relatively high amount of cystine, S-sulfocysteine, and cysteic acid. S-sulfocysteine was present in the free state and also combined in peptides where its amount exceeded that of cystine. This supports the author's hypothesis on keratin decomposition by dermatophytes. According to this hypothesis, the fungus excretes sulfite which cleaves the disulfide bonds of keratin to cysteine and S-sulfocysteine. The substrate, denatured by sulfitolysis, is then more easily digestible by fungal proteases. Besides S-sulfocysteine, comparable amounts of cysteic acid were found in all analyses. This compound most probably originated by air oxidation of sulfur amino acids in the alkaline cultivation fluid.", "PMID": 969572}
{"id": "pubmed23n0032_7576", "title": "[Protein crystals and tubuli bundles in yeasts cells. III. Identification of crystals as alcohol dehydrogenase (ADH)].", "content": "Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical: (1) The three proteins have the same amino acid composition and subunit molecular weight; (2) active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158 000 D; (3) the native H protein and a crystal fraction stabilized by Zn++ and dialyzed against EDTA possess ADH activity.", "contents": "[Protein crystals and tubuli bundles in yeasts cells. III. Identification of crystals as alcohol dehydrogenase (ADH)]. Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical: (1) The three proteins have the same amino acid composition and subunit molecular weight; (2) active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158 000 D; (3) the native H protein and a crystal fraction stabilized by Zn++ and dialyzed against EDTA possess ADH activity.", "PMID": 969573}
{"id": "pubmed23n0032_7577", "title": "[Changes in the polysaccharide biosynthesis of Candida guilliermondii induced by diphenylamine].", "content": "Diphenylamine (DPA) inhibits the formation of glucan in Candida guilliermondii while stimulating that of mannan. The total polysaccharide of cells decreases when growing in the presence of DPA. These effects are dependent on the concentration of DPA. Compared with 14 structure-like substances, DPA was found to have the strongest activity.", "contents": "[Changes in the polysaccharide biosynthesis of Candida guilliermondii induced by diphenylamine]. Diphenylamine (DPA) inhibits the formation of glucan in Candida guilliermondii while stimulating that of mannan. The total polysaccharide of cells decreases when growing in the presence of DPA. These effects are dependent on the concentration of DPA. Compared with 14 structure-like substances, DPA was found to have the strongest activity.", "PMID": 969574}
{"id": "pubmed23n0032_7578", "title": "[Microscopic studies of Streptomyces hygroscopicus growth kinetics].", "content": "Growth kinetics, branch formation, and cytological properties of mycelial growth of Streptomyces hygroscopicus on solid media were investigated by phase-contrast microscopy using a microculture method. Measurements were made on taken photographs of the growing hyphae from the beginning of spore germination up to maximal 18 hours. The specific growth rate of the germ tube was much higher than the specific growth rate of the mycelium. The doubling time of the total length of the mycelial hyphae and the doubling time of branch formation was quite the same for the period investigated. After a short time of outgrowth each individual hypha grows at a constant rate, i.e. the length increases linearly, but the growth kinetics of the whole mycelium becomes exponentially by branching. It seems, that nucleoids only divide in that part of a hypha between the tip and the nearest branch. A cell unit (1.4-1.9 mum on different media) could be calculated by the length of a hypha and the number of nucleoids. A hypha is growing with the cell unit at the tip or with the polar cap only. No interkalary growth in length could be found. Branches were formed only up to about 100 mum from the tip on complex medium and up to about 50 mum from the tip on mineral salt medium. A simple method of mycelial growth has been developed. Some properties showing the connection between Streptomycetes and prokaryotic organisms on one hand and hyphal growing fungi on the other hand are discussed.", "contents": "[Microscopic studies of Streptomyces hygroscopicus growth kinetics]. Growth kinetics, branch formation, and cytological properties of mycelial growth of Streptomyces hygroscopicus on solid media were investigated by phase-contrast microscopy using a microculture method. Measurements were made on taken photographs of the growing hyphae from the beginning of spore germination up to maximal 18 hours. The specific growth rate of the germ tube was much higher than the specific growth rate of the mycelium. The doubling time of the total length of the mycelial hyphae and the doubling time of branch formation was quite the same for the period investigated. After a short time of outgrowth each individual hypha grows at a constant rate, i.e. the length increases linearly, but the growth kinetics of the whole mycelium becomes exponentially by branching. It seems, that nucleoids only divide in that part of a hypha between the tip and the nearest branch. A cell unit (1.4-1.9 mum on different media) could be calculated by the length of a hypha and the number of nucleoids. A hypha is growing with the cell unit at the tip or with the polar cap only. No interkalary growth in length could be found. Branches were formed only up to about 100 mum from the tip on complex medium and up to about 50 mum from the tip on mineral salt medium. A simple method of mycelial growth has been developed. Some properties showing the connection between Streptomycetes and prokaryotic organisms on one hand and hyphal growing fungi on the other hand are discussed.", "PMID": 969575}
{"id": "pubmed23n0032_7579", "title": "Cellulolytic activity of aerobic soil actinomycetes.", "content": "The cellulolytic activity of several aerobic soil actinomycetes against insoluble cellulose and soluble cellulose derivatives (CMC-carboxymethylcellulose) was studied. From the soil, 8 actinomycete strains were isolated after enrichment growth and purification on the same synthetic medium. The actinomycete strains were able to degrade insoluble cellulose, with the production of cellobiose and various oligosaccharide intermediates as degradation products, indicating the random attack of the cellulose chain. The actinomycete strains showed also a great activity against soluble cellulose (CMC). The viscosity of CMC solutions decreased rapidly and was followed by an increase in reducing compounds. The degree of substitution of CMC solutions had an effect on the degradation by the actinomycetes. The degree of polymerization did not affect the rate of hydrolysis, however.", "contents": "Cellulolytic activity of aerobic soil actinomycetes. The cellulolytic activity of several aerobic soil actinomycetes against insoluble cellulose and soluble cellulose derivatives (CMC-carboxymethylcellulose) was studied. From the soil, 8 actinomycete strains were isolated after enrichment growth and purification on the same synthetic medium. The actinomycete strains were able to degrade insoluble cellulose, with the production of cellobiose and various oligosaccharide intermediates as degradation products, indicating the random attack of the cellulose chain. The actinomycete strains showed also a great activity against soluble cellulose (CMC). The viscosity of CMC solutions decreased rapidly and was followed by an increase in reducing compounds. The degree of substitution of CMC solutions had an effect on the degradation by the actinomycetes. The degree of polymerization did not affect the rate of hydrolysis, however.", "PMID": 969580}
{"id": "pubmed23n0032_7580", "title": "[Mediastinoscopy--indications, efficiency, and morphologic findings in more than 2250 investigations (author's transl)].", "content": "2276 diagnostic mediastinoscopies were performed and their efficiency was analysed. Except from patients with bronchial carcinomas, in 80% of all cases pathologic findings with diagnostic significance could be found in the lymph-nodes. CARLENS mediastinoscopy proved to be less efficient in the prognostic evaluation of bronchial carcinomas. In 521 patients with bronchial carcinomas only 146 (28%) showed mediastinal lymph-node metastasis detected by mediastinoscopy. But in the remaining 375 cases the operation revealed in 51.5% the occurence of perihilar metastatic lymph-nodes. The most important morphological findings in lymph-nodes and their differentialdiagnostical problems are discussed with reference to sarcoidosis, sarcoid-like reaction, tuberculosis, and silicosis.", "contents": "[Mediastinoscopy--indications, efficiency, and morphologic findings in more than 2250 investigations (author's transl)]. 2276 diagnostic mediastinoscopies were performed and their efficiency was analysed. Except from patients with bronchial carcinomas, in 80% of all cases pathologic findings with diagnostic significance could be found in the lymph-nodes. CARLENS mediastinoscopy proved to be less efficient in the prognostic evaluation of bronchial carcinomas. In 521 patients with bronchial carcinomas only 146 (28%) showed mediastinal lymph-node metastasis detected by mediastinoscopy. But in the remaining 375 cases the operation revealed in 51.5% the occurence of perihilar metastatic lymph-nodes. The most important morphological findings in lymph-nodes and their differentialdiagnostical problems are discussed with reference to sarcoidosis, sarcoid-like reaction, tuberculosis, and silicosis.", "PMID": 969685}
{"id": "pubmed23n0032_7581", "title": "[The effects of preoperative treatment and physical training to operability in patients with bronchial carcinomas (author's transl)].", "content": "Training effects of preoperative treatment, especially of breathing exercises in 52 patients with bronchial carcinoma were controlled by bodyplethysmography, measurements of the thoracic and abdominal circumferences in inspiration and expiration before and after the training program. In patients with obstructive ventilation disorders the expiratory resistance came to be reduced significantly. The mobility of the thoracic wall and of diaphragm increased in all patient-groups, but not in patients with restrictive ventilation disorders. After introducing the complex program the complication rate seems to drop to half of the previous rate.", "contents": "[The effects of preoperative treatment and physical training to operability in patients with bronchial carcinomas (author's transl)]. Training effects of preoperative treatment, especially of breathing exercises in 52 patients with bronchial carcinoma were controlled by bodyplethysmography, measurements of the thoracic and abdominal circumferences in inspiration and expiration before and after the training program. In patients with obstructive ventilation disorders the expiratory resistance came to be reduced significantly. The mobility of the thoracic wall and of diaphragm increased in all patient-groups, but not in patients with restrictive ventilation disorders. After introducing the complex program the complication rate seems to drop to half of the previous rate.", "PMID": 969686}
{"id": "pubmed23n0032_7582", "title": "[Interrelations between otorhinolaryngologic and chronic nonspecific respiratory diseases (author's transl)].", "content": "1000 patients, suffering from chronic nonspecific respiratory diseases, were investigated for otorhinolaryngologic diseases. The frequency of pathologic findings such as nose deformities, sinusitis, rhinitis, pharyngitis, tonsillitis, respiratory hypersensitivity and their treatment results are evaluated aiming to find out common causative factors in the pathogenesis of these upper and lower airway diseases. A careful diagnosis and subsequent treatment of otorhinolaryngologic diseases is required to improve the therapeutic results of the basic respiratory disease.", "contents": "[Interrelations between otorhinolaryngologic and chronic nonspecific respiratory diseases (author's transl)]. 1000 patients, suffering from chronic nonspecific respiratory diseases, were investigated for otorhinolaryngologic diseases. The frequency of pathologic findings such as nose deformities, sinusitis, rhinitis, pharyngitis, tonsillitis, respiratory hypersensitivity and their treatment results are evaluated aiming to find out common causative factors in the pathogenesis of these upper and lower airway diseases. A careful diagnosis and subsequent treatment of otorhinolaryngologic diseases is required to improve the therapeutic results of the basic respiratory disease.", "PMID": 969687}
{"id": "pubmed23n0032_7583", "title": "[Prognostic evaluation of the absolute lymphopenia during prednisolone therapy of chronic pulmonary sarcoidosis (author's transl)].", "content": "From a controlled prednisolone therapy trial 130 patients with chronic pulmonary sarcoidosis who had already finished their drug-therapy were evaluated. The relapse rate was 28.5% within the following two years. It is obvious, that the long-time prognosis of cases with very low absolute numbers of lymphocytes in the peripheral blood (1000 and less/mul) is significantly poorer than in all other cases.", "contents": "[Prognostic evaluation of the absolute lymphopenia during prednisolone therapy of chronic pulmonary sarcoidosis (author's transl)]. From a controlled prednisolone therapy trial 130 patients with chronic pulmonary sarcoidosis who had already finished their drug-therapy were evaluated. The relapse rate was 28.5% within the following two years. It is obvious, that the long-time prognosis of cases with very low absolute numbers of lymphocytes in the peripheral blood (1000 and less/mul) is significantly poorer than in all other cases.", "PMID": 969688}
{"id": "pubmed23n0032_7584", "title": "[Parental influence to the smoking habits of juveniles (author's transl)].", "content": "A questionnaire was administered to 640 juveniles to get informed about their and their parents smoking behavior. 424 questionnaires could be evaluated, the results and conclusions are reported. Parental smoking habits, especially of their father's are very important influencing factors for the smoking behavior of their children. In their efforts to reduce or to stop smoling the juveniles are looking and seeking for qualified informations as well as support by friends. Physicians, parents, teachers and educators play an important role as key-persons in this respect.", "contents": "[Parental influence to the smoking habits of juveniles (author's transl)]. A questionnaire was administered to 640 juveniles to get informed about their and their parents smoking behavior. 424 questionnaires could be evaluated, the results and conclusions are reported. Parental smoking habits, especially of their father's are very important influencing factors for the smoking behavior of their children. In their efforts to reduce or to stop smoling the juveniles are looking and seeking for qualified informations as well as support by friends. Physicians, parents, teachers and educators play an important role as key-persons in this respect.", "PMID": 969689}
{"id": "pubmed23n0032_7585", "title": "[Rehabilitation of asthmatic patients in the chest hospital (author's transl)].", "content": "Modern chest hospitals offer favourable conditions for the rehabilitation of asthmatic patients. In the Central Hospital for Heart- and Lung Diseases at Bad Berka 421 patients with asthma were rehabilitated under clinical conditions in 1972 and 1973. Their rehabilitation was based on individualized long-term plans for treatment taking in consideration environmental factors which might have triggered the attacks. We report on experiences with diagnostic and therapeutic procedures and the results of vocational rehabilitation. 284 patients (67.4%) could return to their work, 4.5% were disabled, 16.5% remained invalids and 11.6% were old-age pensioners or children. The results depended on age, on the type of asthma, on the severity of cardio-pulmonary impairment, on certain concomitant diseases and on the patients cooperation. Possibilities for elimination of factors starting an attack sometimes proved decisive. For the maintenance of rehabilitatory success subsequent ambulatory long-term care by a specialist is provided.", "contents": "[Rehabilitation of asthmatic patients in the chest hospital (author's transl)]. Modern chest hospitals offer favourable conditions for the rehabilitation of asthmatic patients. In the Central Hospital for Heart- and Lung Diseases at Bad Berka 421 patients with asthma were rehabilitated under clinical conditions in 1972 and 1973. Their rehabilitation was based on individualized long-term plans for treatment taking in consideration environmental factors which might have triggered the attacks. We report on experiences with diagnostic and therapeutic procedures and the results of vocational rehabilitation. 284 patients (67.4%) could return to their work, 4.5% were disabled, 16.5% remained invalids and 11.6% were old-age pensioners or children. The results depended on age, on the type of asthma, on the severity of cardio-pulmonary impairment, on certain concomitant diseases and on the patients cooperation. Possibilities for elimination of factors starting an attack sometimes proved decisive. For the maintenance of rehabilitatory success subsequent ambulatory long-term care by a specialist is provided.", "PMID": 969690}
{"id": "pubmed23n0032_7586", "title": "[Bronchopneumonia and weather in the territory of Leipzig (author's transl)].", "content": "621 autopsied deaths by bronchopneumonia in the territory of Leipzig, basing on emphysema of the lungs. Showed an annual course of the mortality rate due to bronchopneumonia with a clear elevation in March and distinct depressions in July, September, and October. Unambiguous relationships between weather and mortality rate in the several seasons could not be proved.", "contents": "[Bronchopneumonia and weather in the territory of Leipzig (author's transl)]. 621 autopsied deaths by bronchopneumonia in the territory of Leipzig, basing on emphysema of the lungs. Showed an annual course of the mortality rate due to bronchopneumonia with a clear elevation in March and distinct depressions in July, September, and October. Unambiguous relationships between weather and mortality rate in the several seasons could not be proved.", "PMID": 969691}
{"id": "pubmed23n0032_7587", "title": "[Diagnosis of disseminated and localized lung diseases by means of transbronchial lung biopsy (author's transl)].", "content": "In 61 patients with mostly diffuse lung diseases 64 transbronchial lung biopsies have been performed by means of rigid biopsy forceps in general anesthesia. 31 of the 36 pathological bioply specimens were diagnostically helpful or represented the clinical diagnosis. Complications were moderate bleeding in three cases and pneumothorax (3 cases). One patient died after myocardial reinfarction occuring under general anesthesia. Transbronchial lung biopsy is indicated when routine methods fail to establish the diagnosis of pulmonary lesions, particularly in patients having higher operative risks. More use of the transbronchial forceps biopsy is expected to improve the diagnostic yield, especially in patients with miliary pulmonary lesions.", "contents": "[Diagnosis of disseminated and localized lung diseases by means of transbronchial lung biopsy (author's transl)]. In 61 patients with mostly diffuse lung diseases 64 transbronchial lung biopsies have been performed by means of rigid biopsy forceps in general anesthesia. 31 of the 36 pathological bioply specimens were diagnostically helpful or represented the clinical diagnosis. Complications were moderate bleeding in three cases and pneumothorax (3 cases). One patient died after myocardial reinfarction occuring under general anesthesia. Transbronchial lung biopsy is indicated when routine methods fail to establish the diagnosis of pulmonary lesions, particularly in patients having higher operative risks. More use of the transbronchial forceps biopsy is expected to improve the diagnostic yield, especially in patients with miliary pulmonary lesions.", "PMID": 969692}
{"id": "pubmed23n0032_7588", "title": "[Hygienic problems during sputum provocation by aerosol (author's transl)].", "content": "An apparatus assembly is described providing hygienic conditions to obtain sputum after aerosol inhalation. It is composed of an ultrasonic nebulizer and a half-opened exhausting hood. The patient's head is placed under the hood which inhibits the delivery of expectorated germs into the environmental space. A continuous air flow in the direction of coughing is leading the sputum particles (droplet nuclei) towards a filter system. After decontamination the air may be let off outwards or back into the room.", "contents": "[Hygienic problems during sputum provocation by aerosol (author's transl)]. An apparatus assembly is described providing hygienic conditions to obtain sputum after aerosol inhalation. It is composed of an ultrasonic nebulizer and a half-opened exhausting hood. The patient's head is placed under the hood which inhibits the delivery of expectorated germs into the environmental space. A continuous air flow in the direction of coughing is leading the sputum particles (droplet nuclei) towards a filter system. After decontamination the air may be let off outwards or back into the room.", "PMID": 969693}
{"id": "pubmed23n0032_7589", "title": "[The use of inhalation tests for the diagnostick of chronic bronchitis in children (author's transl)].", "content": "In chronic non-specific lung diseases (CNSLD) an increased bronchial reaction to histamine aerosol inhalation is already well known. It was decided then to study the reactivity of bronchi to histamine inhalation in children within the epidemiological investigation on the influence of air pollution on the respiratory system of children. The aim of the study was to determine how in healthy population of children, living only in differently polluted environments, the frequency of the positive tests correlates with history of lung diseases, some pathological symptoms, level of air pollution etc. Results of the inhalation tests were also compared with other functional results of respiratory system, like: spirometrical, pneumo-tachographical, body-plethysmographical measurements, tests of mechanics of respiration, determination of lung transfer factor for CO etc. Numerous other laboratory determinations (level of alpha-antitrypsine, and x-ray examinations) were also performed and compared with the inhalation tests. In spite of expectations in the so called epidemiological \"positives\" as absolute lack of reaction to histamine was observed more frequently. Children with initial airways resistances were also weeker reacting to histamine provocation. Some parameters of lung function in children with hyperreactivity to histamine proved to be initially somewhat worse. The authors assume that some environmental factors may influence by occurrence the reactivity of air-ways provocation by inhalation. The need of general agreement and unification for the technique and interpretation of provocation tests was stressed out. The usefulness of the application of inhalatory tests in epidemiological studies on healthy populations needs further discussion.", "contents": "[The use of inhalation tests for the diagnostick of chronic bronchitis in children (author's transl)]. In chronic non-specific lung diseases (CNSLD) an increased bronchial reaction to histamine aerosol inhalation is already well known. It was decided then to study the reactivity of bronchi to histamine inhalation in children within the epidemiological investigation on the influence of air pollution on the respiratory system of children. The aim of the study was to determine how in healthy population of children, living only in differently polluted environments, the frequency of the positive tests correlates with history of lung diseases, some pathological symptoms, level of air pollution etc. Results of the inhalation tests were also compared with other functional results of respiratory system, like: spirometrical, pneumo-tachographical, body-plethysmographical measurements, tests of mechanics of respiration, determination of lung transfer factor for CO etc. Numerous other laboratory determinations (level of alpha-antitrypsine, and x-ray examinations) were also performed and compared with the inhalation tests. In spite of expectations in the so called epidemiological \"positives\" as absolute lack of reaction to histamine was observed more frequently. Children with initial airways resistances were also weeker reacting to histamine provocation. Some parameters of lung function in children with hyperreactivity to histamine proved to be initially somewhat worse. The authors assume that some environmental factors may influence by occurrence the reactivity of air-ways provocation by inhalation. The need of general agreement and unification for the technique and interpretation of provocation tests was stressed out. The usefulness of the application of inhalatory tests in epidemiological studies on healthy populations needs further discussion.", "PMID": 969694}
{"id": "pubmed23n0032_7590", "title": "[Basic-diagnostic-criterions of respiratory insufficiency--results of a community work of the working group \"pathophysiology of breathing\" (author's transl)].", "content": "11 laboratories of the working group \"Pathophysiology of Breathing\" cooperated with standardized methods to obtain generally admitted practical criterions for the definition of respiratory insufficiency. Function parameters of 2800 examined persons in different diagnostic groups were tested by manual statistical analysis and computed by data processing system. which were assessed with regard to their usefulness as criterions for respiratory insufficiency. The arterial O2-partial pressure proved to be the most crucial criterion of respiratory insufficiency permitting objective evidence of a manifest respiratory insufficiency by hypoxeamia under standard efforts with a bicycle-ergometer. The screening parameters of ventilation such as vital capacity and forced exspiratory volume are suitable as subjective rapid methods for a quantitative estimation of one type of respiratory insufficiency: the ventilation disturbance. Investigations, which were carried out with a higher apparatus expense like the estimation of residual volume, the functional residual capacity, and the arterial CO2-partial pressure permit the further differentiation of causes of the respiratory insufficiency and the separation between respiratory partial- and global insufficiency. The parameters of gas exchange: O2-consumption, respiratory quotient, and the screening parameter of cardiocirculation heart rate are not or only limited useful as criterions of respiratory insufficiency alone.", "contents": "[Basic-diagnostic-criterions of respiratory insufficiency--results of a community work of the working group \"pathophysiology of breathing\" (author's transl)]. 11 laboratories of the working group \"Pathophysiology of Breathing\" cooperated with standardized methods to obtain generally admitted practical criterions for the definition of respiratory insufficiency. Function parameters of 2800 examined persons in different diagnostic groups were tested by manual statistical analysis and computed by data processing system. which were assessed with regard to their usefulness as criterions for respiratory insufficiency. The arterial O2-partial pressure proved to be the most crucial criterion of respiratory insufficiency permitting objective evidence of a manifest respiratory insufficiency by hypoxeamia under standard efforts with a bicycle-ergometer. The screening parameters of ventilation such as vital capacity and forced exspiratory volume are suitable as subjective rapid methods for a quantitative estimation of one type of respiratory insufficiency: the ventilation disturbance. Investigations, which were carried out with a higher apparatus expense like the estimation of residual volume, the functional residual capacity, and the arterial CO2-partial pressure permit the further differentiation of causes of the respiratory insufficiency and the separation between respiratory partial- and global insufficiency. The parameters of gas exchange: O2-consumption, respiratory quotient, and the screening parameter of cardiocirculation heart rate are not or only limited useful as criterions of respiratory insufficiency alone.", "PMID": 969695}
{"id": "pubmed23n0032_7591", "title": "[Differentiation and identification of mycobacteria by means of thin-layer chromatography of their lipids. II. Application for the classification of slowly growing mycobacteria: M. avium and M. intracellulare (author's transl)].", "content": "Experiences with the thin-layer chromatography of mycobacterial lipids for the differentiation of the species M. avium and M. intracellulare are described. Details of the procedure used have been published in a recent paper. The 61 strains investigated belong to 16 different serotypes. Twenty of the 21 strains of M. avium present three distinct lipid patterns corresponding to the serotypes 1, 2 and 3. One strain had an aberrant lipid pattern suggesting that serotyping was at fault. The results obtained with 40 M. intracellulare strains of the serotypes III, IV, VI, VII, Davis, Watson, Howell, Chance, Boone, Yandle, Wilson, Altman and Arnold show that a differentiation by thin-layer chromatography of their lipids--using two different developing solvents--is possible. In allbut one case there was a complete correspondence between classification by lipid analysis and the results of serological typing. The distinction between M. avium and M intracellulare on the basis of species specific lipid patterns is not possible. This supports the conclusion that M. avium and M. intracellulare appear to be the same species having a number of distinctive serotypes.", "contents": "[Differentiation and identification of mycobacteria by means of thin-layer chromatography of their lipids. II. Application for the classification of slowly growing mycobacteria: M. avium and M. intracellulare (author's transl)]. Experiences with the thin-layer chromatography of mycobacterial lipids for the differentiation of the species M. avium and M. intracellulare are described. Details of the procedure used have been published in a recent paper. The 61 strains investigated belong to 16 different serotypes. Twenty of the 21 strains of M. avium present three distinct lipid patterns corresponding to the serotypes 1, 2 and 3. One strain had an aberrant lipid pattern suggesting that serotyping was at fault. The results obtained with 40 M. intracellulare strains of the serotypes III, IV, VI, VII, Davis, Watson, Howell, Chance, Boone, Yandle, Wilson, Altman and Arnold show that a differentiation by thin-layer chromatography of their lipids--using two different developing solvents--is possible. In allbut one case there was a complete correspondence between classification by lipid analysis and the results of serological typing. The distinction between M. avium and M intracellulare on the basis of species specific lipid patterns is not possible. This supports the conclusion that M. avium and M. intracellulare appear to be the same species having a number of distinctive serotypes.", "PMID": 969696}
{"id": "pubmed23n0032_7592", "title": "[Physician's decision making in evaluation of tuberculous patients assessed by a questionnaire (author's transl)].", "content": "By a questionnaire 88 chest physicians were asked anonymously for their opinion about 15 cases of tuberculosis with various problems (choice of chemotherapeutic regimens, duration of treatment, rehabilitation and other aspects) in regard to first or repeated treatment and in chronic tuberculosis. It can be demonstrated, that in the overwhelming number the evaluation of the cases is done with safety and that the recommendations of the advisory board for chest diseases and tuberculosis are noticed.", "contents": "[Physician's decision making in evaluation of tuberculous patients assessed by a questionnaire (author's transl)]. By a questionnaire 88 chest physicians were asked anonymously for their opinion about 15 cases of tuberculosis with various problems (choice of chemotherapeutic regimens, duration of treatment, rehabilitation and other aspects) in regard to first or repeated treatment and in chronic tuberculosis. It can be demonstrated, that in the overwhelming number the evaluation of the cases is done with safety and that the recommendations of the advisory board for chest diseases and tuberculosis are noticed.", "PMID": 969697}
{"id": "pubmed23n0032_7593", "title": "[The influence of rifampicin on the lymphocyte stimulation (author's transl)].", "content": "In 7 PHA--and PPD stimulated lymphocyte cultures from healthy human donors the maintained immunosuppressive effect of Rifampicin was investigated. Concentrations of 5-200 mug RMP/ml culture medium were used. A significant decrease in the transformation rate was found at 20 mug/ml and higher concentrations. A vitality examination (trypan blue staining) on lymphocytes after 72 hours cultivation in a RMP-containing medium with the same concentrations showed no increase of stained cells as compared with the controls. It is suggested that the observed inhibition of lymphocyte reactivity is to be attributed to an immunosuppressive effect of RMP and not to a toxic influence.", "contents": "[The influence of rifampicin on the lymphocyte stimulation (author's transl)]. In 7 PHA--and PPD stimulated lymphocyte cultures from healthy human donors the maintained immunosuppressive effect of Rifampicin was investigated. Concentrations of 5-200 mug RMP/ml culture medium were used. A significant decrease in the transformation rate was found at 20 mug/ml and higher concentrations. A vitality examination (trypan blue staining) on lymphocytes after 72 hours cultivation in a RMP-containing medium with the same concentrations showed no increase of stained cells as compared with the controls. It is suggested that the observed inhibition of lymphocyte reactivity is to be attributed to an immunosuppressive effect of RMP and not to a toxic influence.", "PMID": 969698}
{"id": "pubmed23n0032_7594", "title": "[A contribution to the symptomatology of diffuse interstitial pulmonary fibrosis (DIPF) (author's transl)].", "content": "Diffuse interstitial pulmonary fibrosis was detected in 64 patients (33 males and 31 females). The diagnosis was obtained in each case from the anamnesis, the clinical findings, the chest X-ray, disturbances of the respiratory function, and from the progress of the disease. The analysis of the material produced the following parameters ranging from indicative to typical: Dry cough was reported by 70% of the patients as being trouble-some. Dyspnoea on exertion and at rest existed in 95%, and gradually increased during the progress of the disease in 76,5% of these cases. On auscultation ear-near crepitant rale was heard in 78% above the affected pulmonary parts. In 62 patients (96,5%) the radiogram showed disseminated streaky-reticular as well as finely to coarsely mottled infiltrations. In more than half of these cases (53%) an increase in severity was observed during the follow-up. A predominantly restrictive disturbance of ventilation existed in 85,5%. Exclusive restriction was found in 65,5%. Effort hypoxemia was detected in 92% of our cases which was especially impressive when the arterial oxygen pressure at rest was within the normal range. The electrocardiographic findings seemed to be less unequivocal. All in all it can be stated that in the synopsis of its manifestation DIPF can be positively identified by clinical means. This fact as well as therapeutical problems are discussed taking available publications into consideration, and respective conclusions are drawn for the clinician.", "contents": "[A contribution to the symptomatology of diffuse interstitial pulmonary fibrosis (DIPF) (author's transl)]. Diffuse interstitial pulmonary fibrosis was detected in 64 patients (33 males and 31 females). The diagnosis was obtained in each case from the anamnesis, the clinical findings, the chest X-ray, disturbances of the respiratory function, and from the progress of the disease. The analysis of the material produced the following parameters ranging from indicative to typical: Dry cough was reported by 70% of the patients as being trouble-some. Dyspnoea on exertion and at rest existed in 95%, and gradually increased during the progress of the disease in 76,5% of these cases. On auscultation ear-near crepitant rale was heard in 78% above the affected pulmonary parts. In 62 patients (96,5%) the radiogram showed disseminated streaky-reticular as well as finely to coarsely mottled infiltrations. In more than half of these cases (53%) an increase in severity was observed during the follow-up. A predominantly restrictive disturbance of ventilation existed in 85,5%. Exclusive restriction was found in 65,5%. Effort hypoxemia was detected in 92% of our cases which was especially impressive when the arterial oxygen pressure at rest was within the normal range. The electrocardiographic findings seemed to be less unequivocal. All in all it can be stated that in the synopsis of its manifestation DIPF can be positively identified by clinical means. This fact as well as therapeutical problems are discussed taking available publications into consideration, and respective conclusions are drawn for the clinician.", "PMID": 969699}
{"id": "pubmed23n0032_7595", "title": "[Environmental aerosol and human health (author's transl)].", "content": "In the modern way of thinking, the influence of environmental aerosol on human health represents a partial problem of the manifold interrelations between biological, chemical, and physical environmental factors in terms of their combined and complex effect on the human organism, including the socio-economic environmental conditions. As regards the air as an environmental factor, two fundamental problems are being dealt with in some detail under this particular aspect: the relation between exposure and effect, and the conception of limiting values for the protection of human health. The traditional conception of \"one cause--one effect\" is inappropriate for dealing with this complex set of problems and has to be replaced by the \"many causes--many effects\" principle while paying heed to nonspecific as well as indirect effects on the health and well-being of man. The difficulties encountered in clarifying the relation between exposure and effect, and in assessing air pollution from the hygienic point of view are pointed out, and an outline of the present state of knowledge and of the experimental and epidemiological methods of research as applied in this field is given. In conclusion the principles for the determination of air-hygienic standards are expounded.", "contents": "[Environmental aerosol and human health (author's transl)]. In the modern way of thinking, the influence of environmental aerosol on human health represents a partial problem of the manifold interrelations between biological, chemical, and physical environmental factors in terms of their combined and complex effect on the human organism, including the socio-economic environmental conditions. As regards the air as an environmental factor, two fundamental problems are being dealt with in some detail under this particular aspect: the relation between exposure and effect, and the conception of limiting values for the protection of human health. The traditional conception of \"one cause--one effect\" is inappropriate for dealing with this complex set of problems and has to be replaced by the \"many causes--many effects\" principle while paying heed to nonspecific as well as indirect effects on the health and well-being of man. The difficulties encountered in clarifying the relation between exposure and effect, and in assessing air pollution from the hygienic point of view are pointed out, and an outline of the present state of knowledge and of the experimental and epidemiological methods of research as applied in this field is given. In conclusion the principles for the determination of air-hygienic standards are expounded.", "PMID": 969700}
{"id": "pubmed23n0032_7596", "title": "[The effect of caffeine on the resorption and various central effects of ethanol].", "content": "Caffeine given in a dose of 400 mg one hour before 1 g/kg ethanol did not influence the course of blood alcohol levels in male volunteers. Furthermore, caffeine did not improve psychomotoric skills impaired by ethanol. Two cups of coffee ingested 30 min. after ethanol (0.5 g/kg) caused a statistically significant increase of blood ethanol levels one hour afterwards (from 0.49 to 0.61%). This may be due to an accelerated absorption of ethanol caused by the ingestion of warm fluid.", "contents": "[The effect of caffeine on the resorption and various central effects of ethanol]. Caffeine given in a dose of 400 mg one hour before 1 g/kg ethanol did not influence the course of blood alcohol levels in male volunteers. Furthermore, caffeine did not improve psychomotoric skills impaired by ethanol. Two cups of coffee ingested 30 min. after ethanol (0.5 g/kg) caused a statistically significant increase of blood ethanol levels one hour afterwards (from 0.49 to 0.61%). This may be due to an accelerated absorption of ethanol caused by the ingestion of warm fluid.", "PMID": 969701}
{"id": "pubmed23n0032_7597", "title": "Coffee consumption and mortality in a community study--Evans Co., Ga.", "content": "Total mortality showed no association with heavy coffee consumption in the four race-sex groups of Evans County. Deaths from coronary heart disease in WM, WF and BM showed no statistically significant differences between the two coffee consuming groups. Sex differences in cerebrovascular death rates, consistent in both races, suggest the possibility for a female excess of stroke deaths among coffee drinkers, and a \"protective\" effect of coffee drinking among males. Thus, in an area of the United States which has been designated the \"Stroke Belt\", neither the cardiovascular nor the cerebrovascular death rates seem strongly nor consistently related to coffee drinking habits. Although the number of deaths (339) is fairly large, representing a 13% mortality in this community over a four and one-half year observation period, the classification in four race-sex groups with further division into the groups with different coffee drinking habits limits each stratum to rather small numbers. In addition, 86 cases of CHD and CVD were diagnosed during lifetime already and, therefore, were excluded from the prospective mortality study. Confidently to refute or confirm the allegations of a detrimental influence of high coffee intake on ischemic heart disease one would need larger numbers. But in the light of our most important finding--that mortality from all causes is not increased in the high coffee consuming group--the finding of increased ischemic heart disease death rates with high coffee consumption would have to be compensated by a provocative, lower rate for other causes of death.", "contents": "Coffee consumption and mortality in a community study--Evans Co., Ga. Total mortality showed no association with heavy coffee consumption in the four race-sex groups of Evans County. Deaths from coronary heart disease in WM, WF and BM showed no statistically significant differences between the two coffee consuming groups. Sex differences in cerebrovascular death rates, consistent in both races, suggest the possibility for a female excess of stroke deaths among coffee drinkers, and a \"protective\" effect of coffee drinking among males. Thus, in an area of the United States which has been designated the \"Stroke Belt\", neither the cardiovascular nor the cerebrovascular death rates seem strongly nor consistently related to coffee drinking habits. Although the number of deaths (339) is fairly large, representing a 13% mortality in this community over a four and one-half year observation period, the classification in four race-sex groups with further division into the groups with different coffee drinking habits limits each stratum to rather small numbers. In addition, 86 cases of CHD and CVD were diagnosed during lifetime already and, therefore, were excluded from the prospective mortality study. Confidently to refute or confirm the allegations of a detrimental influence of high coffee intake on ischemic heart disease one would need larger numbers. But in the light of our most important finding--that mortality from all causes is not increased in the high coffee consuming group--the finding of increased ischemic heart disease death rates with high coffee consumption would have to be compensated by a provocative, lower rate for other causes of death.", "PMID": 969703}
{"id": "pubmed23n0032_7598", "title": "[The effect of caffeine on the serum insulin level during intravenous glucose tolerance test in patients with chemical diabetes].", "content": "Nine mild male diabetic patients were studied by intravenous glucose tolerance tests with and without pretreatment with caffeine. The dose of caffeine was 90 mg given as 195 mg of caffeine citrate. The parameters measured were blood glucose and serum insulin. Caffeine given 2 hours previously did not affect the mean glucose disappearance rate of the group, however, those with the highest rates did show a reduced rate after caffeine. Insulin levels showed a decrease particularly during the first five minutes after glucose. This finding was present particularly in those patients who exhibited the larger first peak. It is possible that a relationship exists between the magnitude of the caffeine effect upon early insulin secretion and the glucose disappearance rate.", "contents": "[The effect of caffeine on the serum insulin level during intravenous glucose tolerance test in patients with chemical diabetes]. Nine mild male diabetic patients were studied by intravenous glucose tolerance tests with and without pretreatment with caffeine. The dose of caffeine was 90 mg given as 195 mg of caffeine citrate. The parameters measured were blood glucose and serum insulin. Caffeine given 2 hours previously did not affect the mean glucose disappearance rate of the group, however, those with the highest rates did show a reduced rate after caffeine. Insulin levels showed a decrease particularly during the first five minutes after glucose. This finding was present particularly in those patients who exhibited the larger first peak. It is possible that a relationship exists between the magnitude of the caffeine effect upon early insulin secretion and the glucose disappearance rate.", "PMID": 969704}
{"id": "pubmed23n0032_7599", "title": "[The effect of coffee and coffee components on the stomach muscle].", "content": "The isolated stomach muscle strip preparation works well when given pure compounds which are stable and non-toxic. When complex test mixtures such as coffee are used, however, the results are more difficult to interpret. This is probably due to non-specific effects of such mixtures, e.g. induction of changes in organ bath pH, etc. In spite of the above remark, differences in the effects of decaffeinated and normal instant coffees were clearly seen. The so-called \"wholesome\" coffees gave results identical to those obtained with normal instant coffee. In general, coffee induced muscle contraction followed by relaxation. Choline (possibly together with potassium) would appear to be responsible for the contractions observed, and caffeine for the relaxation.", "contents": "[The effect of coffee and coffee components on the stomach muscle]. The isolated stomach muscle strip preparation works well when given pure compounds which are stable and non-toxic. When complex test mixtures such as coffee are used, however, the results are more difficult to interpret. This is probably due to non-specific effects of such mixtures, e.g. induction of changes in organ bath pH, etc. In spite of the above remark, differences in the effects of decaffeinated and normal instant coffees were clearly seen. The so-called \"wholesome\" coffees gave results identical to those obtained with normal instant coffee. In general, coffee induced muscle contraction followed by relaxation. Choline (possibly together with potassium) would appear to be responsible for the contractions observed, and caffeine for the relaxation.", "PMID": 969705}
{"id": "pubmed23n0032_7600", "title": "[Danger to human health from mycotoxins].", "content": "The review paper summarizes our knowledge on the influence of the intake of mycotoxin containing foodstuffs on human health. The greatest attention is given to the aflatoxins. The results of epidemiological studies on the occurrence of primary liver cancer in Thailand and different regions of middle and southern Africa are described.", "contents": "[Danger to human health from mycotoxins]. The review paper summarizes our knowledge on the influence of the intake of mycotoxin containing foodstuffs on human health. The greatest attention is given to the aflatoxins. The results of epidemiological studies on the occurrence of primary liver cancer in Thailand and different regions of middle and southern Africa are described.", "PMID": 969706}
{"id": "pubmed23n0032_7601", "title": "The effect of chromium III on the intravenous glucose tolerance of rats given diets of different protein values.", "content": "The effect of chromium administration on glucose utilization was investigated in 32 rats fed either high or low protein diet. In rats fed a low protein diet of NDpE% 4.3 the glucose removal rate increased significantly following the administration of chromium. However, the administration of chromium has no effect on the glucose removal rate in rats fed high protein diet of NDpE% 10.1. The glucose removal rate of rats kept on a diet of high protein value free from chromium was significantly higher than those kept on a low protein diet. Chromium deficiency did not appear to be the only responsible cause for the impaired glucose tolerance in animals fed low protein diet. It could be concluded that both chromium supplementation and protein ingestion promote insulin secretion.", "contents": "The effect of chromium III on the intravenous glucose tolerance of rats given diets of different protein values. The effect of chromium administration on glucose utilization was investigated in 32 rats fed either high or low protein diet. In rats fed a low protein diet of NDpE% 4.3 the glucose removal rate increased significantly following the administration of chromium. However, the administration of chromium has no effect on the glucose removal rate in rats fed high protein diet of NDpE% 10.1. The glucose removal rate of rats kept on a diet of high protein value free from chromium was significantly higher than those kept on a low protein diet. Chromium deficiency did not appear to be the only responsible cause for the impaired glucose tolerance in animals fed low protein diet. It could be concluded that both chromium supplementation and protein ingestion promote insulin secretion.", "PMID": 969707}
{"id": "pubmed23n0032_7602", "title": "[The sulfur dioxide in various population groups in the German Federal Republic].", "content": "In direct nutrition surveys, mostly with precise weighing methods, the SO2 intake was estimated in 10 groups of population in the FRG. The averages in the groups are in comparison with the FAO/WHO recommendation very low. This result was also found in most of the people in surveys. From group to group there is a wide range and in the groups too. The highest intake in sulfur dioxide has a group of winegrowers with 0.88 mg/kg body weight and day.", "contents": "[The sulfur dioxide in various population groups in the German Federal Republic]. In direct nutrition surveys, mostly with precise weighing methods, the SO2 intake was estimated in 10 groups of population in the FRG. The averages in the groups are in comparison with the FAO/WHO recommendation very low. This result was also found in most of the people in surveys. From group to group there is a wide range and in the groups too. The highest intake in sulfur dioxide has a group of winegrowers with 0.88 mg/kg body weight and day.", "PMID": 969709}
{"id": "pubmed23n0032_7603", "title": "[Interactions between nutrition and drugs].", "content": "It is only recently that the question of interaction between nutrition and drugs has been investigated. Generally there are two ways by which such an interaction may occur. On the one hand the food-intake and its composition may influence the effects of drugs by altering their pharmacokinetics. In most cases the abosrption of drugs and thereby also their biological effects are decreased when they are taken together with food, by only a few drugs the opposite effects have been observed. Furthermore the biotransformation of drugs may be inhibited by giving a diet poor in proteins. A diet rich in meat or in vegetables may also influence the urine pH in the more acid or basic direction and in this way the renal excretion of drugs may be changed considerably since drugs are generally either weak organic acid or bases. On the other hand drugs may also interfere with the availability and utilization of certain nutrients e.g. vitamins, electrolytes or trace elements. Such interaction could be observed when giving for example antibiotics, alcohol, contraceptives, anticonvulsives or laxatives over a long period. Deficiency of certain nutritional factors or even diseases can then be the consequence of such interactions.", "contents": "[Interactions between nutrition and drugs]. It is only recently that the question of interaction between nutrition and drugs has been investigated. Generally there are two ways by which such an interaction may occur. On the one hand the food-intake and its composition may influence the effects of drugs by altering their pharmacokinetics. In most cases the abosrption of drugs and thereby also their biological effects are decreased when they are taken together with food, by only a few drugs the opposite effects have been observed. Furthermore the biotransformation of drugs may be inhibited by giving a diet poor in proteins. A diet rich in meat or in vegetables may also influence the urine pH in the more acid or basic direction and in this way the renal excretion of drugs may be changed considerably since drugs are generally either weak organic acid or bases. On the other hand drugs may also interfere with the availability and utilization of certain nutrients e.g. vitamins, electrolytes or trace elements. Such interaction could be observed when giving for example antibiotics, alcohol, contraceptives, anticonvulsives or laxatives over a long period. Deficiency of certain nutritional factors or even diseases can then be the consequence of such interactions.", "PMID": 969708}
{"id": "pubmed23n0032_7604", "title": "Phytochemical and nutritional studies on pigeon pea and kidney bean cultivated in Egypt.", "content": "1. The preliminary phytochemical screening of the two seeds established the presence of carbohydrates and/or glycosides, flavnoids, unsaturated sterols and/or triterpenes, saponins, trypsin inhibitors and haemagglutinins. In addition, it established the absence of cardenolides, tannins, alkaloids and oxidase enzyme. 2. Certain pharmacopoeial constants, including moisture, ash, acid-insoluble ash, water-soluble ash and crude fibre were determined. 3. The two seeds were subjected to successive extractions with different organic solvents such as petroleum ether (50-70 degrees C), diethyl ether, chloroform and ethyl alcohol. The successive yields of extractives were determined. Examination of the crude extracts showed that petroleum ether extract contained sterols and/or triterpenes, while ether, chloroform, and ethyl alcohol extracts contained reducing substances. 4. General analysis of the two seeds for proteins, fats, carbohydrates, fibre and ash contents were carried out and the results were given in g/100 g dry seeds. Pigeon pea contained 25.2 g protein, 170 mg calcium and 8.9 mg iron. The protein content of kidney bean was 23 g, while calcium and iron contents were 134 mg and 8.02 mg respectively. 5. Extractions of the proteins using different solvents such as cold water, hot water, saline buffer pH 7 and sodium hydroxide was the best extractant. 6. The amino-acid content of the two seeds, whether raw or cooked, showed that they were deficient in methionine, cystine and tryptophan. Other essential amino acids were present in amounts higher than that given by the FAO provisional pattern. 7. Cooking the seeds by the popular methods used in the country resulted in an increase in the amounts of the amino acids, threonine, leucine and isoleucine, while the other amino acids present remained unchanged or decreased. It was also observed that cooking the seeds destroyed the trypsin inhibitors and haemagglutinins found in the two seeds.", "contents": "Phytochemical and nutritional studies on pigeon pea and kidney bean cultivated in Egypt. 1. The preliminary phytochemical screening of the two seeds established the presence of carbohydrates and/or glycosides, flavnoids, unsaturated sterols and/or triterpenes, saponins, trypsin inhibitors and haemagglutinins. In addition, it established the absence of cardenolides, tannins, alkaloids and oxidase enzyme. 2. Certain pharmacopoeial constants, including moisture, ash, acid-insoluble ash, water-soluble ash and crude fibre were determined. 3. The two seeds were subjected to successive extractions with different organic solvents such as petroleum ether (50-70 degrees C), diethyl ether, chloroform and ethyl alcohol. The successive yields of extractives were determined. Examination of the crude extracts showed that petroleum ether extract contained sterols and/or triterpenes, while ether, chloroform, and ethyl alcohol extracts contained reducing substances. 4. General analysis of the two seeds for proteins, fats, carbohydrates, fibre and ash contents were carried out and the results were given in g/100 g dry seeds. Pigeon pea contained 25.2 g protein, 170 mg calcium and 8.9 mg iron. The protein content of kidney bean was 23 g, while calcium and iron contents were 134 mg and 8.02 mg respectively. 5. Extractions of the proteins using different solvents such as cold water, hot water, saline buffer pH 7 and sodium hydroxide was the best extractant. 6. The amino-acid content of the two seeds, whether raw or cooked, showed that they were deficient in methionine, cystine and tryptophan. Other essential amino acids were present in amounts higher than that given by the FAO provisional pattern. 7. Cooking the seeds by the popular methods used in the country resulted in an increase in the amounts of the amino acids, threonine, leucine and isoleucine, while the other amino acids present remained unchanged or decreased. It was also observed that cooking the seeds destroyed the trypsin inhibitors and haemagglutinins found in the two seeds.", "PMID": 969710}
{"id": "pubmed23n0032_7605", "title": "[The catabolism of infused maltose in man].", "content": "The use of intravenously administered maltose was tested in 9 healthy human subjects and 3 insulin-dependent diabetic patients. The concentration of the blood sugar has not been influenced by the administered maltose. The concentration of maltose in the blood increases up to 170 mg/100 ml blood depending on the rate of the maltose infusion. The excretion of maltose in the urinis correlated with the applied dosis and with the blood maltose concentration. Under our experimental conditions 20 to 30% of the administered maltose have been excreted and 7.5 to 23.4% have been oxidized within 8 hours. The highest rate of degradation was about 40 mg maltose/min/human subject and is reached 2 hours later than the peak concentration of maltose in the blood. The metabolism of maltose is reduced in insulin-dependent diabetic patients. In these patients only 3% of the applied maltose have been oxidized and 51% excreted in the urin within 8 hours. Therefore, this disaccharide cannot be recommended as carbohydrate source of parenteral nutrition in insulin-dependent diabetic patients. The balance of intravenously administered maltose is not satisfactory in healthy adult humans, too. Infusion of maltose solutions have no real advantages over the infusions of oligosaccharide solutions.", "contents": "[The catabolism of infused maltose in man]. The use of intravenously administered maltose was tested in 9 healthy human subjects and 3 insulin-dependent diabetic patients. The concentration of the blood sugar has not been influenced by the administered maltose. The concentration of maltose in the blood increases up to 170 mg/100 ml blood depending on the rate of the maltose infusion. The excretion of maltose in the urinis correlated with the applied dosis and with the blood maltose concentration. Under our experimental conditions 20 to 30% of the administered maltose have been excreted and 7.5 to 23.4% have been oxidized within 8 hours. The highest rate of degradation was about 40 mg maltose/min/human subject and is reached 2 hours later than the peak concentration of maltose in the blood. The metabolism of maltose is reduced in insulin-dependent diabetic patients. In these patients only 3% of the applied maltose have been oxidized and 51% excreted in the urin within 8 hours. Therefore, this disaccharide cannot be recommended as carbohydrate source of parenteral nutrition in insulin-dependent diabetic patients. The balance of intravenously administered maltose is not satisfactory in healthy adult humans, too. Infusion of maltose solutions have no real advantages over the infusions of oligosaccharide solutions.", "PMID": 969711}
{"id": "pubmed23n0032_7606", "title": "[Effect of maltose, following long-term intravenous infusion, on the metabolism].", "content": "6 healthy subjects received a 20% electrolyte containing maltose solution over a period of 6 hours. The concentration of maltose rose up to 109 (76-215 mg/100 ml). During the infusion period 3,7 (1,9-7,7) g maltose were excreted. The concentration of glucose and insulin was not altered, although the renal glucose loss was 20,2 (7,5-38,7) g. In the same period 49,8 +/- 13,2 mval potassium and 135,4 +/- 35 mval sodium were eliminated. Maltose induced an osmotic diuresis which was not caused by hyperglycemia. Such a type of osmotic substance has not been described before.", "contents": "[Effect of maltose, following long-term intravenous infusion, on the metabolism]. 6 healthy subjects received a 20% electrolyte containing maltose solution over a period of 6 hours. The concentration of maltose rose up to 109 (76-215 mg/100 ml). During the infusion period 3,7 (1,9-7,7) g maltose were excreted. The concentration of glucose and insulin was not altered, although the renal glucose loss was 20,2 (7,5-38,7) g. In the same period 49,8 +/- 13,2 mval potassium and 135,4 +/- 35 mval sodium were eliminated. Maltose induced an osmotic diuresis which was not caused by hyperglycemia. Such a type of osmotic substance has not been described before.", "PMID": 969712}
{"id": "pubmed23n0032_7607", "title": "[Metabolic effect of a parenterally administered fat emulsion with middle-chain triglycerides in healthy men].", "content": "Within 3 hours, 10 healthy male volunteers were infused 500 ml of a 5 percent emulsion in which 25 percent of the fat proportion had been replaced by MCT-fats. As expected, the ketone body concentration in the blood rose, while pyruvate remained constant and lactate dropped. The results show that, basically, a MCT-containing fat infusion is suited for parenteral nutrition and, because of their specific properties, medium chain triglycerides may be used as rapid energy donators.", "contents": "[Metabolic effect of a parenterally administered fat emulsion with middle-chain triglycerides in healthy men]. Within 3 hours, 10 healthy male volunteers were infused 500 ml of a 5 percent emulsion in which 25 percent of the fat proportion had been replaced by MCT-fats. As expected, the ketone body concentration in the blood rose, while pyruvate remained constant and lactate dropped. The results show that, basically, a MCT-containing fat infusion is suited for parenteral nutrition and, because of their specific properties, medium chain triglycerides may be used as rapid energy donators.", "PMID": 969713}
{"id": "pubmed23n0032_7608", "title": "[Tests with human volunteers on parenteral utilization of maltose].", "content": "Intravenous infusions of maltose were performed using human volunteers. Four volunteers received maltose in a dose of 0.25 g/kg bodyweight and hour during eight hours. A follow-up period of three hours was added. Six volunteers received maltose in a dose of 0.125 g/kg bodyweight and hour during twelve hours. Only with the lower dose of maltose (0.125 g/kg b.w.) a steady state is reached after six hour continuous infusion. However even under these conditions maltose concentration in blood reaches the high concentration of 70 mg/100 ml. Using the double infusion rate, no steady state is attained when the infusions lasted for eight hours, despite maltose concentration in blood measured 150 mg/100 ml at this time. By measuring different metabolic parameters (fatty acid concentration, phosphate concentration) it is shown that parenterally applicated maltose is metabolized in the human. On the other hand, adverse reactions were not observed. The concentrations of uric acid and bilirubin remain constant and the activity of SGOT is not altered. Renal excretion of sugar measures 25-35% of the maltose administered parenterally. It is concluded that the glucose in urine stems from direct intra tubular hydrolysis of maltose achieved by the neutral maltase of the kidneys. The lack of attaining constant blood concentration for maltose during the infusions and the high renal loss of sugar shows that maltose is not suited as the single substrate for parenteral nutrition. However, there remains the possibility to use maltose in combination with glucose substitutes. The metabolic behaviour of maltose is similar to glucose, it differs from glucose substitutes.", "contents": "[Tests with human volunteers on parenteral utilization of maltose]. Intravenous infusions of maltose were performed using human volunteers. Four volunteers received maltose in a dose of 0.25 g/kg bodyweight and hour during eight hours. A follow-up period of three hours was added. Six volunteers received maltose in a dose of 0.125 g/kg bodyweight and hour during twelve hours. Only with the lower dose of maltose (0.125 g/kg b.w.) a steady state is reached after six hour continuous infusion. However even under these conditions maltose concentration in blood reaches the high concentration of 70 mg/100 ml. Using the double infusion rate, no steady state is attained when the infusions lasted for eight hours, despite maltose concentration in blood measured 150 mg/100 ml at this time. By measuring different metabolic parameters (fatty acid concentration, phosphate concentration) it is shown that parenterally applicated maltose is metabolized in the human. On the other hand, adverse reactions were not observed. The concentrations of uric acid and bilirubin remain constant and the activity of SGOT is not altered. Renal excretion of sugar measures 25-35% of the maltose administered parenterally. It is concluded that the glucose in urine stems from direct intra tubular hydrolysis of maltose achieved by the neutral maltase of the kidneys. The lack of attaining constant blood concentration for maltose during the infusions and the high renal loss of sugar shows that maltose is not suited as the single substrate for parenteral nutrition. However, there remains the possibility to use maltose in combination with glucose substitutes. The metabolic behaviour of maltose is similar to glucose, it differs from glucose substitutes.", "PMID": 969714}
{"id": "pubmed23n0032_7609", "title": "[Gas chromatographic analysis of amino acids in food].", "content": "1. The methylester derivative of DNP-amino acid was examined by gas-chromatography and amino acid shown in fig. 1 was detected. 2. The gaschromatography was more sensitive than the ion-exchange chromatography, but detectable peaks were few, and it must be further studied. 3. The measurements of five amino acids in food were almost coincident with those obtained by the ion-exchange method in literatures.", "contents": "[Gas chromatographic analysis of amino acids in food]. 1. The methylester derivative of DNP-amino acid was examined by gas-chromatography and amino acid shown in fig. 1 was detected. 2. The gaschromatography was more sensitive than the ion-exchange chromatography, but detectable peaks were few, and it must be further studied. 3. The measurements of five amino acids in food were almost coincident with those obtained by the ion-exchange method in literatures.", "PMID": 969715}
{"id": "pubmed23n0032_7610", "title": "The protein nutritive value of proteolan, a by-product of starch industry.", "content": "The nutrient contents as well as the amino-acid pattern of the proteolan by-product were determined. The results showed the presence of 18 amino acids. Most of the essential amino acids are existing in optimal concentration with exception of the sulfur-containing amino acids which showed deficiencies. The animal experiments indicated high figures for the digestibility coefficient and low figures for both the biological and net protein-utilization values. Mixing the proteolan in equal proportion with barley rootlets, a by-product of malting, leads to a noticeable increase in the biological value.", "contents": "The protein nutritive value of proteolan, a by-product of starch industry. The nutrient contents as well as the amino-acid pattern of the proteolan by-product were determined. The results showed the presence of 18 amino acids. Most of the essential amino acids are existing in optimal concentration with exception of the sulfur-containing amino acids which showed deficiencies. The animal experiments indicated high figures for the digestibility coefficient and low figures for both the biological and net protein-utilization values. Mixing the proteolan in equal proportion with barley rootlets, a by-product of malting, leads to a noticeable increase in the biological value.", "PMID": 969716}
{"id": "pubmed23n0032_7611", "title": "[Fatty-acid pattern of serum lipids (standards for mothers, placentas and infants)].", "content": "The standard distribution of the fatty acids in the total lipids of serum was gaschromatographically defined in the respective umbilical cord blood of 24 mothers and their newborn babies, as well as in 20 placentas. The same examinations were made with 30 newborn babies on the day of birth, 14 children in the newborn stage, 22 young babies between 3 and 10 weeks old and 16 older babies between 4 and 12 months old. In the umbilical cord mixed blood of another collective (n = 10) the separation of the total serum lipids in the individual fatty fractions (neutral lipids, phospholipids, cholesterol esters) was also undertaken. The results were compared and existing differences were discussed. It was further established that an advance towards the adult's fatty acid pattern already takes place at an age between 4 and 12 months.", "contents": "[Fatty-acid pattern of serum lipids (standards for mothers, placentas and infants)]. The standard distribution of the fatty acids in the total lipids of serum was gaschromatographically defined in the respective umbilical cord blood of 24 mothers and their newborn babies, as well as in 20 placentas. The same examinations were made with 30 newborn babies on the day of birth, 14 children in the newborn stage, 22 young babies between 3 and 10 weeks old and 16 older babies between 4 and 12 months old. In the umbilical cord mixed blood of another collective (n = 10) the separation of the total serum lipids in the individual fatty fractions (neutral lipids, phospholipids, cholesterol esters) was also undertaken. The results were compared and existing differences were discussed. It was further established that an advance towards the adult's fatty acid pattern already takes place at an age between 4 and 12 months.", "PMID": 969717}
{"id": "pubmed23n0032_7612", "title": "[Leading symptom: the M-gradient in electrophoresis].", "content": "A survey is given on development, proof and clinical importance of an M-gradient which is to be demarcated in the electrophoresis. A M-gradient refers to an excessive increase of the number of monoclonic immunoglobulins (= paraproteins). As investigation method the electrophoresis on agar or agarose, respectively, is best suited. The immunoelectrophoresis is necessary for the further differentiation into several classes and types as well as for the demarcation of pseudo-M-gradients. The proof of a M-gradient alone does not allow a diagnosis. M-gradients obligatorily appear in the plasmocytoma, in Waldenstroem's macroglobulinaemia and associated neoplasias of the lympho-plasma-cellular tissue. However, M-gradients can also be observed in carcinomas, chronic liver diseases and chronic inflammatory processes as well as in clinically healthy persons in individual cases. The differentiation according to the cause of the M-gradient is possible only then, when the total clinical picture is taken into consideration, in individual cases, however, according to the course of the disease.", "contents": "[Leading symptom: the M-gradient in electrophoresis]. A survey is given on development, proof and clinical importance of an M-gradient which is to be demarcated in the electrophoresis. A M-gradient refers to an excessive increase of the number of monoclonic immunoglobulins (= paraproteins). As investigation method the electrophoresis on agar or agarose, respectively, is best suited. The immunoelectrophoresis is necessary for the further differentiation into several classes and types as well as for the demarcation of pseudo-M-gradients. The proof of a M-gradient alone does not allow a diagnosis. M-gradients obligatorily appear in the plasmocytoma, in Waldenstroem's macroglobulinaemia and associated neoplasias of the lympho-plasma-cellular tissue. However, M-gradients can also be observed in carcinomas, chronic liver diseases and chronic inflammatory processes as well as in clinically healthy persons in individual cases. The differentiation according to the cause of the M-gradient is possible only then, when the total clinical picture is taken into consideration, in individual cases, however, according to the course of the disease.", "PMID": 969762}
{"id": "pubmed23n0032_7613", "title": "[Psychological examinations in essential hypertension].", "content": "The influence of psychic factors on the blood pressure has been known since decenniums. In order to establish the psychic peculiarities of 25 male and 25 female patients, the complaint questionnaire of H\u00f6ck and the Minnesota Multiphasic Personality Inventory (MMPI) was used. In 16 male and 21 female patients with the MMPI a neurosis could be established psychometrically. In female neurotic hypertonics the duration of the disease was three times longer than in non-neurotic hypertonics. The treatment of hypertension should not be exhausted in pharmacotherapy, but should also include the clearing up of the actual situation of life.", "contents": "[Psychological examinations in essential hypertension]. The influence of psychic factors on the blood pressure has been known since decenniums. In order to establish the psychic peculiarities of 25 male and 25 female patients, the complaint questionnaire of H\u00f6ck and the Minnesota Multiphasic Personality Inventory (MMPI) was used. In 16 male and 21 female patients with the MMPI a neurosis could be established psychometrically. In female neurotic hypertonics the duration of the disease was three times longer than in non-neurotic hypertonics. The treatment of hypertension should not be exhausted in pharmacotherapy, but should also include the clearing up of the actual situation of life.", "PMID": 969763}
{"id": "pubmed23n0032_7614", "title": "[Coexistence of psychoneuroses with organic diseases].", "content": "The increasing number of the coexistence of psychoneuroses with organic diseases may lead to several diagnostic difficulties and errors. It may occur that the physician by mistake diagnoses only an organic disease. In other cases it may also occur that the physician makes an opposite mistake assuming only a psychoneurosis. The aim of this paper was the discussion of these two possibilities of mistake on the basis of the own experience of the author.", "contents": "[Coexistence of psychoneuroses with organic diseases]. The increasing number of the coexistence of psychoneuroses with organic diseases may lead to several diagnostic difficulties and errors. It may occur that the physician by mistake diagnoses only an organic disease. In other cases it may also occur that the physician makes an opposite mistake assuming only a psychoneurosis. The aim of this paper was the discussion of these two possibilities of mistake on the basis of the own experience of the author.", "PMID": 969764}
{"id": "pubmed23n0032_7615", "title": "[Training effects in the treatment of neuroses].", "content": "The whole kinetotherapeutic programme of a psychotherapeutic department--without systematic training in the sense of athletics--in more than half of the cases surprisingly leads to physiologic changes, as they are to be expected in a submaximal training. In significant correlation with this the quality of the rule of the circulatory parameter investigated (pulse rate) improves. In the majority of cases in which positive training and regulation effects developed the psychotherapeutic results were clearly positive, too. On the other hand only in less than half of all cases with clear psychotherapeutic improvement training effects could be proved. The psychologic effects of training are based on a reaction complex the most essential components of which are described.", "contents": "[Training effects in the treatment of neuroses]. The whole kinetotherapeutic programme of a psychotherapeutic department--without systematic training in the sense of athletics--in more than half of the cases surprisingly leads to physiologic changes, as they are to be expected in a submaximal training. In significant correlation with this the quality of the rule of the circulatory parameter investigated (pulse rate) improves. In the majority of cases in which positive training and regulation effects developed the psychotherapeutic results were clearly positive, too. On the other hand only in less than half of all cases with clear psychotherapeutic improvement training effects could be proved. The psychologic effects of training are based on a reaction complex the most essential components of which are described.", "PMID": 969765}
{"id": "pubmed23n0032_7616", "title": "[Ambulatory psychotherapy at the medical department of a district policlinic].", "content": "From the psychotherapeutic unit of the department of internal diseases of a district outpatient department some experiences and recognitions are reported. The autogenic training and the group psychotherapy are the methodical centre. 200 non-selected psychotherapeutic cases are analysed with regard to sex, age, profession and diagnostics of the neurotic symptomatology.", "contents": "[Ambulatory psychotherapy at the medical department of a district policlinic]. From the psychotherapeutic unit of the department of internal diseases of a district outpatient department some experiences and recognitions are reported. The autogenic training and the group psychotherapy are the methodical centre. 200 non-selected psychotherapeutic cases are analysed with regard to sex, age, profession and diagnostics of the neurotic symptomatology.", "PMID": 969766}
{"id": "pubmed23n0032_7617", "title": "[Incidence and significance of psychovegetative syndromes--demonstration on an example of an internal medicine diagnostic station].", "content": "It is reported on 215 patients in whom after detailed somatic and psychodiagnostic examination in a ward of internal diseases the diagnosis of neurosis was made. The cases in question were 33.3% of the whole number of patients during a selected period. In the majority of cases (74%) organic functional disturbances were diagnosed, above all cardiovascular and gastro-enterological syndromes. The screening diagnostics especially with the help of questionaires of complaints allowed of a comparatively certain conclusion to the existence of a psychovegetative syndrome. Altogether 51 patients exhibited the picture of a functional organic linkage syndrome, i.e. in an organic disease a characteristic functional syndrome remained ascertained. The duration of the diseases was always several years, they were chronified, mostly by iatrogenic influences and labelled by an organic pseudodiagnosis. Among the therapeutic measures possible in practice already the diagnosing of a neurosis represents a decisive step.", "contents": "[Incidence and significance of psychovegetative syndromes--demonstration on an example of an internal medicine diagnostic station]. It is reported on 215 patients in whom after detailed somatic and psychodiagnostic examination in a ward of internal diseases the diagnosis of neurosis was made. The cases in question were 33.3% of the whole number of patients during a selected period. In the majority of cases (74%) organic functional disturbances were diagnosed, above all cardiovascular and gastro-enterological syndromes. The screening diagnostics especially with the help of questionaires of complaints allowed of a comparatively certain conclusion to the existence of a psychovegetative syndrome. Altogether 51 patients exhibited the picture of a functional organic linkage syndrome, i.e. in an organic disease a characteristic functional syndrome remained ascertained. The duration of the diseases was always several years, they were chronified, mostly by iatrogenic influences and labelled by an organic pseudodiagnosis. Among the therapeutic measures possible in practice already the diagnosing of a neurosis represents a decisive step.", "PMID": 969767}
{"id": "pubmed23n0032_7618", "title": "[Clinical aspects and pathogenesis of paramyloidosis in plasmacytoma].", "content": "In the first phase of inhalation due to the sedimentation of droplets against the walls of the atomizer arises a high loss of fluid in the apparatus. When the air flow was regular a high scattering volume of the consumed fluid quantity from the remedy store container was obtained. In the short-time inhalation this method cannot be used for the estimation of the quantity of medicaments applied to the patient. An exact dosage is possible only by measuring the fog flow and when the exact density of fog is known. When the presuppositions mentioned are not given, the informations about the virtual density of fog which was calculated by means of the analysis of regression, may be obtained.", "contents": "[Clinical aspects and pathogenesis of paramyloidosis in plasmacytoma]. In the first phase of inhalation due to the sedimentation of droplets against the walls of the atomizer arises a high loss of fluid in the apparatus. When the air flow was regular a high scattering volume of the consumed fluid quantity from the remedy store container was obtained. In the short-time inhalation this method cannot be used for the estimation of the quantity of medicaments applied to the patient. An exact dosage is possible only by measuring the fog flow and when the exact density of fog is known. When the presuppositions mentioned are not given, the informations about the virtual density of fog which was calculated by means of the analysis of regression, may be obtained.", "PMID": 969768}
{"id": "pubmed23n0032_7619", "title": "[Obesity and diabetes-morbidity--a prospective 5-year study].", "content": "In a prospective 5-year-study the development of adiposity was tested in a population group consisting of about 30,000 persons. It was shown that during the time of investigation the population decreased in its total number, the consumption of easily digestible carbohydrates and meat, however, increased. As it was established in 1968, the proportion of the adipose persons (more than 20% of the Broca-weight) and the percental average deviation from the Broca-weight (calculated in decennium classes) was more than in the comparable population groups in the GDR. Contrary to expectation the examination further showed that on an average men exhibited a trend to the increase in weight, but on the average women decreased in weight. In all age groups (with the exception of the 60- to 69-year-old persons) the proportion of obese persons was significantly lower in 1973 than in 1968. Unchangedly adipose are the diabetics who also have a by far higher average weight than the healthy population and who up to now do not reveal an inclination to the decrease in weight. With 2.9% the average frequency of diabetes is also adequately high.", "contents": "[Obesity and diabetes-morbidity--a prospective 5-year study]. In a prospective 5-year-study the development of adiposity was tested in a population group consisting of about 30,000 persons. It was shown that during the time of investigation the population decreased in its total number, the consumption of easily digestible carbohydrates and meat, however, increased. As it was established in 1968, the proportion of the adipose persons (more than 20% of the Broca-weight) and the percental average deviation from the Broca-weight (calculated in decennium classes) was more than in the comparable population groups in the GDR. Contrary to expectation the examination further showed that on an average men exhibited a trend to the increase in weight, but on the average women decreased in weight. In all age groups (with the exception of the 60- to 69-year-old persons) the proportion of obese persons was significantly lower in 1973 than in 1968. Unchangedly adipose are the diabetics who also have a by far higher average weight than the healthy population and who up to now do not reveal an inclination to the decrease in weight. With 2.9% the average frequency of diabetes is also adequately high.", "PMID": 969769}
{"id": "pubmed23n0032_7620", "title": "[Functional and urinary analysis diagnosis of pyelonephritis].", "content": "For the practicing physician pyelonephritis is a frequently observed clinical picture which still has its diagnostic problems. With the help of a step programme a reasonable diagnostics is striven for which gives the possibility of an early recognition in the general surgery and a far-reaching clarification of the clinical picture in the out-patient department.", "contents": "[Functional and urinary analysis diagnosis of pyelonephritis]. For the practicing physician pyelonephritis is a frequently observed clinical picture which still has its diagnostic problems. With the help of a step programme a reasonable diagnostics is striven for which gives the possibility of an early recognition in the general surgery and a far-reaching clarification of the clinical picture in the out-patient department.", "PMID": 969770}
{"id": "pubmed23n0032_7621", "title": "[Roentgen diagnosis of pyelonephritis].", "content": "With the help of special literature and the results of own investigations the usability and evidence of radiological examination methods in the diagnosis of pyelonephritis are described. The high value of urography in the ascertainment of predisposing factors for pyelonephritis is emphasized. Subject of nearer considerations is the characteristic radiological feature of chronic pyelonephritis in its different developmental stages. Especially in connection with the decent radiological changes in the early forms of chronical pyelonephritis is referred to the necessity of an optimal collaboration between clinician and radiologist.", "contents": "[Roentgen diagnosis of pyelonephritis]. With the help of special literature and the results of own investigations the usability and evidence of radiological examination methods in the diagnosis of pyelonephritis are described. The high value of urography in the ascertainment of predisposing factors for pyelonephritis is emphasized. Subject of nearer considerations is the characteristic radiological feature of chronic pyelonephritis in its different developmental stages. Especially in connection with the decent radiological changes in the early forms of chronical pyelonephritis is referred to the necessity of an optimal collaboration between clinician and radiologist.", "PMID": 969771}
{"id": "pubmed23n0032_7622", "title": "[Chemotherapy in pyelonephritis].", "content": "In the present paper the most important general directions of the chemotherapy of the acute as well as the chronic pyelonephritis are explained. In adult age actual acute pyelonephritides are rare. In most cases acute attacks of chronic pyelonephritides are in question. Also in the acute pyelonephritides for the initial therapy the application of highly effective chemotherapeutics is recommended which lead to high tissue and serum levels. The exclusive application of nitrofurantoin or an only symptomatic therapy is not indicated in acute pyelonephritis. Only a consequent therapy of the acute form of the course gives the possibility to reduce the number of chronic pyelonephritides. The most favourable conditions for the treatment of chronic pyelonephritis with the aim of stopping the chronic process or eventually even a cure are given by the long-term therapy. For the initial therapy according to the antibiogramme a tissue effective chemotherapeutic remedy is administered. This was followed by a long-term prophylaxis of about 6 months. Taking into consideration the therapeutic mechanisms of the preparations and the severity of the clinical picture always the least toxical antibacterial remedies should be administered. The most important chemotherapeutics for the therapy of pyelonephritis, their side-effects and the peculiarities of their use in renal insufficiency are discussed.", "contents": "[Chemotherapy in pyelonephritis]. In the present paper the most important general directions of the chemotherapy of the acute as well as the chronic pyelonephritis are explained. In adult age actual acute pyelonephritides are rare. In most cases acute attacks of chronic pyelonephritides are in question. Also in the acute pyelonephritides for the initial therapy the application of highly effective chemotherapeutics is recommended which lead to high tissue and serum levels. The exclusive application of nitrofurantoin or an only symptomatic therapy is not indicated in acute pyelonephritis. Only a consequent therapy of the acute form of the course gives the possibility to reduce the number of chronic pyelonephritides. The most favourable conditions for the treatment of chronic pyelonephritis with the aim of stopping the chronic process or eventually even a cure are given by the long-term therapy. For the initial therapy according to the antibiogramme a tissue effective chemotherapeutic remedy is administered. This was followed by a long-term prophylaxis of about 6 months. Taking into consideration the therapeutic mechanisms of the preparations and the severity of the clinical picture always the least toxical antibacterial remedies should be administered. The most important chemotherapeutics for the therapy of pyelonephritis, their side-effects and the peculiarities of their use in renal insufficiency are discussed.", "PMID": 969772}
{"id": "pubmed23n0032_7623", "title": "[Urolithiasis and its conservative treatment based on analysis of the calculi].", "content": "The exact analysis of urinary calculi is the condition for an aimed conservative therapy of urolithiasis. As standardized method of the analysis of urinary calculi the X-ray diffraction was introduced in the GDR. This investigation method allows a qualitative and quantitative analysis of the concrements. The possibilities of the conservative therapy of urolithiasis were explained. To this conservative treatment belong the treatment of a colic, the removal of a calculus, the litholysis and the prophylaxis of recidivations.", "contents": "[Urolithiasis and its conservative treatment based on analysis of the calculi]. The exact analysis of urinary calculi is the condition for an aimed conservative therapy of urolithiasis. As standardized method of the analysis of urinary calculi the X-ray diffraction was introduced in the GDR. This investigation method allows a qualitative and quantitative analysis of the concrements. The possibilities of the conservative therapy of urolithiasis were explained. To this conservative treatment belong the treatment of a colic, the removal of a calculus, the litholysis and the prophylaxis of recidivations.", "PMID": 969773}
{"id": "pubmed23n0032_7624", "title": "[Immunopathogenesis of glomerulonephritis].", "content": "After an introductory description of some morphological points of view the author enters into the mechanisms of the immunopathogenetic lesion in different forms of glomerulonephritis. Hereby Goodpasture's syndrome, the acute post-streptococcal nephritis, the membranoproliferative glomerulonephritis, the sclerosing forms and the so-called glomerulonephritis with minimal changes are particularly taken into consideration. It is tried to describe the clinical course and the pathomorphological changes.", "contents": "[Immunopathogenesis of glomerulonephritis]. After an introductory description of some morphological points of view the author enters into the mechanisms of the immunopathogenetic lesion in different forms of glomerulonephritis. Hereby Goodpasture's syndrome, the acute post-streptococcal nephritis, the membranoproliferative glomerulonephritis, the sclerosing forms and the so-called glomerulonephritis with minimal changes are particularly taken into consideration. It is tried to describe the clinical course and the pathomorphological changes.", "PMID": 969774}
{"id": "pubmed23n0032_7625", "title": "[Comparative clinical and histological studies in the diagnosis of isolated proteinuria and hematuria].", "content": "In 311 patients with clinical suspicion to glomerulonephritis biopsies of the kidneys were performed. In these cases in 82% the histological or tentative diagnosis, respectively, of a glomerulonephritis could be made. As diseases preceding the glomerulonephritis relapsing tonsillitides are occupying the first place, whereas scarlet fever, otitides, furunculoses and sinusitides were observed more infrequently. Clinically cases of oligosymptomatic glomerulonephritis were more frequently observed than monosymptomatic ones. One fifth of the patients exhibited a restricted renal function or a proteinuria of 3 g/24 hours, in which case proliferatively sclerosing, diffusely proliferative and membranaceous forms occupied the first place.", "contents": "[Comparative clinical and histological studies in the diagnosis of isolated proteinuria and hematuria]. In 311 patients with clinical suspicion to glomerulonephritis biopsies of the kidneys were performed. In these cases in 82% the histological or tentative diagnosis, respectively, of a glomerulonephritis could be made. As diseases preceding the glomerulonephritis relapsing tonsillitides are occupying the first place, whereas scarlet fever, otitides, furunculoses and sinusitides were observed more infrequently. Clinically cases of oligosymptomatic glomerulonephritis were more frequently observed than monosymptomatic ones. One fifth of the patients exhibited a restricted renal function or a proteinuria of 3 g/24 hours, in which case proliferatively sclerosing, diffusely proliferative and membranaceous forms occupied the first place.", "PMID": 969775}
{"id": "pubmed23n0032_7626", "title": "[Clinical aspects of glomerulonephritis].", "content": "With the help of 260 bioptically ascertained cases of glomerulonephritis clinical characteristics for individual forms of glomerulonephritis are explained. Specific laboratory findings and functional tests are correlated to the bioptic findings and their valency for the individual groups of diagnosis discussed. As a result of the investigation 99% of all cases of disease may be recognized only by the examination of the urine, taking into consideration the interference of the individual symptoms.", "contents": "[Clinical aspects of glomerulonephritis]. With the help of 260 bioptically ascertained cases of glomerulonephritis clinical characteristics for individual forms of glomerulonephritis are explained. Specific laboratory findings and functional tests are correlated to the bioptic findings and their valency for the individual groups of diagnosis discussed. As a result of the investigation 99% of all cases of disease may be recognized only by the examination of the urine, taking into consideration the interference of the individual symptoms.", "PMID": 969776}
{"id": "pubmed23n0032_7627", "title": "[Treatment of glomerulonephritis].", "content": "Issuing from the importance of the glomerulonephritis and the different spontaneous course of the certain morphological forms of glomerulonephritis recommendations are given for the indication of a therapy of glomerulonephritis. For the prednisone, indomethazin, azathioprin and cyclophosphamide treatment therapeutic schemata proved in practice are recommended. The results of the therapy of 32 cases of glomerulonephritis treated with prednisone and of 125 cases treated with indomethazin are described. Two observations of a five-year-course in a rapid progressive glomerulonephritis and in a Goodpasture syndrome refer to a probably favourable therapeutic effect of azathioprin. Finally it is referred to the advantages of an additional immunofluorescence-histological examination of the renal tissue in 50 cases of glomerulonephritis observed during the course.", "contents": "[Treatment of glomerulonephritis]. Issuing from the importance of the glomerulonephritis and the different spontaneous course of the certain morphological forms of glomerulonephritis recommendations are given for the indication of a therapy of glomerulonephritis. For the prednisone, indomethazin, azathioprin and cyclophosphamide treatment therapeutic schemata proved in practice are recommended. The results of the therapy of 32 cases of glomerulonephritis treated with prednisone and of 125 cases treated with indomethazin are described. Two observations of a five-year-course in a rapid progressive glomerulonephritis and in a Goodpasture syndrome refer to a probably favourable therapeutic effect of azathioprin. Finally it is referred to the advantages of an additional immunofluorescence-histological examination of the renal tissue in 50 cases of glomerulonephritis observed during the course.", "PMID": 969777}
{"id": "pubmed23n0032_7628", "title": "[Immature-cell leukoses in the adult age].", "content": "In form of a survey the author enters into selected questions of the etiopathogenesis, the pre-leucaemic phase, the epidemiology, the cytochemical subdivision, the clinic and therapy of undifferentiated cell leucaemia in adult age. The number of two-year sojourns in a hospital and other data are reported on the basis of an evaluation of the signature ledges of the clinical histories in the district Dresden. A study concerning the therapy carried out on 331 patients from six clinics of the GDR emphasizes the value of the polychemotherapy, in which cases according to the COAP- and ViDaP-scheme the results of the treatment are somewhat more unfavourable, compared with the results in the newest combinations of other authors.", "contents": "[Immature-cell leukoses in the adult age]. In form of a survey the author enters into selected questions of the etiopathogenesis, the pre-leucaemic phase, the epidemiology, the cytochemical subdivision, the clinic and therapy of undifferentiated cell leucaemia in adult age. The number of two-year sojourns in a hospital and other data are reported on the basis of an evaluation of the signature ledges of the clinical histories in the district Dresden. A study concerning the therapy carried out on 331 patients from six clinics of the GDR emphasizes the value of the polychemotherapy, in which cases according to the COAP- and ViDaP-scheme the results of the treatment are somewhat more unfavourable, compared with the results in the newest combinations of other authors.", "PMID": 969778}
{"id": "pubmed23n0032_7629", "title": "[Effect of some choleretics on the hepatic flow and its composition in man].", "content": "Four choleretics, selected at random from the German list of medical preparations, were tested with regards to their influence on hepatic bile production and bile composition. The investigations were performed on patients with a T-drainage in the ductus choledochus after bile duct revision. Bile was collected after fasting (90 min) and after administration of the drugs to be tested (3 hours). The choleretics were administered orally in a dosage two to five times more than the recommended one. Besides the bile volume bile acids, cholesterol, bilirubin, bicarbonate, potassium, sodium and calcium were determined. None of the parameters could be definitely influenced by these drugs. On the other hand a pronounced increase in the bile flow as well as the expected change in the other parameters could be observed after standardized secretion-stimulation which had been additionally carried out in half of the patients (dehydrocholic acid, i.v., taurocholic acid intraduodenally, secretin i.v., meals).", "contents": "[Effect of some choleretics on the hepatic flow and its composition in man]. Four choleretics, selected at random from the German list of medical preparations, were tested with regards to their influence on hepatic bile production and bile composition. The investigations were performed on patients with a T-drainage in the ductus choledochus after bile duct revision. Bile was collected after fasting (90 min) and after administration of the drugs to be tested (3 hours). The choleretics were administered orally in a dosage two to five times more than the recommended one. Besides the bile volume bile acids, cholesterol, bilirubin, bicarbonate, potassium, sodium and calcium were determined. None of the parameters could be definitely influenced by these drugs. On the other hand a pronounced increase in the bile flow as well as the expected change in the other parameters could be observed after standardized secretion-stimulation which had been additionally carried out in half of the patients (dehydrocholic acid, i.v., taurocholic acid intraduodenally, secretin i.v., meals).", "PMID": 969779}
{"id": "pubmed23n0032_7630", "title": "[Immune response to HBsAg, HBcAg and e-antigen in patients with acute hepatitis and HBsAg carriers with and without liver diseases].", "content": "Humoral and/or cell-mediated (CMI) immune responses to HBAg components, human and rabbit liver specific proteins (HLP and RLP) and tuberculin were tested in patients with acute virus B and non-B-hepatitis, asymptomatic HBsAg carriers and HBsAg positive chronic active hepatitis (CAH). Furthermore, the presence of HBsAg, HBcAg and/or \"e\"-antigen has been studied in patients with sera and/or liver tissue. Asymptomatic HBsAg carriers are characterized by a status of immunological tolerance against HBsAg. HBcAg in liver nuclei could not be detected. All sera were positive for anti-HBc, some had anti \"e\". - Patients with uneventful acute virus-B-hepatitis developed CMI against HBsAg 4-6 weeks and anti-HBs 4-6 months after onset of the disease. Acute virus hepatitis without detectable HBsAg are defined as non-B-hepatitis by negative humoral and cell-mediated immune reaction against HBsAg 1-12 months after onset of the disease. - Patients with type B chronic active hepatitis are characterized by inadequate CMI against HBsAg without immune elimination of virus and virusantigens. Acute and chronic type-B-hepatitis showed temporary or constant CMI against HLP. These findings suggest an alteration or a carrier function of membrane antigens of virus infected hepatocytes or an induction of new membrane antigens by a virus. The results indicate that recovery from type B-hepatitis is associated with the ability to elicit a specific immune response to HBsAg. Furthermore immune responses to virus, virus antigens and virusinfected hepatocytes seemed to be the pathogenic principle of virus induced acute and chronic liver diseases.", "contents": "[Immune response to HBsAg, HBcAg and e-antigen in patients with acute hepatitis and HBsAg carriers with and without liver diseases]. Humoral and/or cell-mediated (CMI) immune responses to HBAg components, human and rabbit liver specific proteins (HLP and RLP) and tuberculin were tested in patients with acute virus B and non-B-hepatitis, asymptomatic HBsAg carriers and HBsAg positive chronic active hepatitis (CAH). Furthermore, the presence of HBsAg, HBcAg and/or \"e\"-antigen has been studied in patients with sera and/or liver tissue. Asymptomatic HBsAg carriers are characterized by a status of immunological tolerance against HBsAg. HBcAg in liver nuclei could not be detected. All sera were positive for anti-HBc, some had anti \"e\". - Patients with uneventful acute virus-B-hepatitis developed CMI against HBsAg 4-6 weeks and anti-HBs 4-6 months after onset of the disease. Acute virus hepatitis without detectable HBsAg are defined as non-B-hepatitis by negative humoral and cell-mediated immune reaction against HBsAg 1-12 months after onset of the disease. - Patients with type B chronic active hepatitis are characterized by inadequate CMI against HBsAg without immune elimination of virus and virusantigens. Acute and chronic type-B-hepatitis showed temporary or constant CMI against HLP. These findings suggest an alteration or a carrier function of membrane antigens of virus infected hepatocytes or an induction of new membrane antigens by a virus. The results indicate that recovery from type B-hepatitis is associated with the ability to elicit a specific immune response to HBsAg. Furthermore immune responses to virus, virus antigens and virusinfected hepatocytes seemed to be the pathogenic principle of virus induced acute and chronic liver diseases.", "PMID": 969780}
{"id": "pubmed23n0032_7631", "title": "[Diffusion disorders in patients with liver cirrhosis].", "content": "In 17 patients with cirrhosis of theliver and in 11 controls the pulmonary diffusing capacity for CO (DLCO) was determined at three different levels of alveolar oxygen tensions. The diffusing capacity of the alveolar membrane (DMCO) and the intra-pulmonary capillary volume (VC) were calculated following the formula given by Roughton and Forster. The following results were obtained: 1) Both DLCO and DMCO were lower (p less than 0,01) in the patient group than in the controls. 2) VC showed larger variations in thepatient group than in the controls (p less than 0,01). The mean values did not differ, however. 3) There was a significant linear correlation (p less than 0,001) between DM and 1/VC in the patient group (DM and VC in % of the predicted value). The results suggest, that a change in the configuration of the capillary bed may be responsible for the transfer defect found in cirrhosis.", "contents": "[Diffusion disorders in patients with liver cirrhosis]. In 17 patients with cirrhosis of theliver and in 11 controls the pulmonary diffusing capacity for CO (DLCO) was determined at three different levels of alveolar oxygen tensions. The diffusing capacity of the alveolar membrane (DMCO) and the intra-pulmonary capillary volume (VC) were calculated following the formula given by Roughton and Forster. The following results were obtained: 1) Both DLCO and DMCO were lower (p less than 0,01) in the patient group than in the controls. 2) VC showed larger variations in thepatient group than in the controls (p less than 0,01). The mean values did not differ, however. 3) There was a significant linear correlation (p less than 0,001) between DM and 1/VC in the patient group (DM and VC in % of the predicted value). The results suggest, that a change in the configuration of the capillary bed may be responsible for the transfer defect found in cirrhosis.", "PMID": 969781}
{"id": "pubmed23n0032_7632", "title": "[Toxic megaileum. Relapse of ulcerative colitis in the ileum following colectomy].", "content": "A 19 year old male patient suffered from ulcerative colitis and was colectomized 5 1/2 years ago. He presented now with an acute illness, characterized by massive discharges from the ileostoma, fever, leucocytosis, meteroism, melaena and a tender abdomen. A severe ulcerative inflammation of the total ileum was found. A \"toxic megaileum\" developed. At an operation the ileum was opened and drained. The patient recoverd under treatment with prednisolone, salazosulfypyridine, parenteral nutrition and Vivasorb. It is suggested that in this case the severe ileitis was due to the same disease which led to the total colectomy 5 1/2 years ago: ulcerative colitis.", "contents": "[Toxic megaileum. Relapse of ulcerative colitis in the ileum following colectomy]. A 19 year old male patient suffered from ulcerative colitis and was colectomized 5 1/2 years ago. He presented now with an acute illness, characterized by massive discharges from the ileostoma, fever, leucocytosis, meteroism, melaena and a tender abdomen. A severe ulcerative inflammation of the total ileum was found. A \"toxic megaileum\" developed. At an operation the ileum was opened and drained. The patient recoverd under treatment with prednisolone, salazosulfypyridine, parenteral nutrition and Vivasorb. It is suggested that in this case the severe ileitis was due to the same disease which led to the total colectomy 5 1/2 years ago: ulcerative colitis.", "PMID": 969782}
{"id": "pubmed23n0032_7633", "title": "[Difficult diagnosis of Crohn's disease].", "content": "It is often difficult to diagnose Crohn's disease because of the numerous clinical symptoms. In a retrospective study from 1963-1975 the initial diagnosis was examined in 101 patients with Crohn's disease. In 22% of the patients the initial diagnosis was correct, in 78% one or more wrong diagnoses were made. The most regular wrong diagnosis was colitis ulcerosa (30%). 41 patients were unnecessarily treated surgically before the correct diagnosis was established. The mean time from the on-set of the symptoms until the correct diagnosis was 3.6 years, the mean time from the first wrong diagnosis to the correct diagnosis, 2.9 years. In the later few years a decrease in wrong diagnoses, an increase in initially correct diagnoses and a shortening of the interval between beginning of symptoms until final diagnosis are recognizable. The possible reasons are discussed.", "contents": "[Difficult diagnosis of Crohn's disease]. It is often difficult to diagnose Crohn's disease because of the numerous clinical symptoms. In a retrospective study from 1963-1975 the initial diagnosis was examined in 101 patients with Crohn's disease. In 22% of the patients the initial diagnosis was correct, in 78% one or more wrong diagnoses were made. The most regular wrong diagnosis was colitis ulcerosa (30%). 41 patients were unnecessarily treated surgically before the correct diagnosis was established. The mean time from the on-set of the symptoms until the correct diagnosis was 3.6 years, the mean time from the first wrong diagnosis to the correct diagnosis, 2.9 years. In the later few years a decrease in wrong diagnoses, an increase in initially correct diagnoses and a shortening of the interval between beginning of symptoms until final diagnosis are recognizable. The possible reasons are discussed.", "PMID": 969783}
{"id": "pubmed23n0032_7634", "title": "[Active transport mechanisms in the intestinal mucosa of the small intestine and their functional disorders].", "content": "The mechanismus of active transport in the small intestinal mucosa are discussed. A survey on the different defects of disturbed absorption is given and examples of 3 basic types of malabsorption are discussed.", "contents": "[Active transport mechanisms in the intestinal mucosa of the small intestine and their functional disorders]. The mechanismus of active transport in the small intestinal mucosa are discussed. A survey on the different defects of disturbed absorption is given and examples of 3 basic types of malabsorption are discussed.", "PMID": 969784}
{"id": "pubmed23n0032_7635", "title": "[Adaptive changes in intestinal mucosa of the small intestine as a result of intraluminal stress].", "content": "Under physiological conditions, the intestinal mucosa is the site of a delicate balance between cell proliferation in the crypt region and cell desquamation at the villus tips. This balance can be deranged by a number of endogenous or exogenous factors, oneof which is the intraluminal contents. This review discusses the effects of different modifications of the luminal milieu on the structure and function of the mucosa. Following intestinal resection or loop transposition, the contents reaching the remnant or the transposed loop differ markedly from those with which they come into contact under normal conditions. Hyperplasia without zonal transformation then develops in the experimental loop. The villi do not become wider, and changes in the strucutre of the epithelium are not observed. There are conflicting resutls in the literature concerning the enzyme activities in the individual enterocytes resulting from this hyperplasia. However, the epithelium its functionally immature, since transport capacities measured in vitro are reduced. On the other hand, the hyperplasia of the mucosa is such that absorptive capacities in vivo, when expressed in terms of intestinal length, are larger than normal. When the intestine is subjected to prolonged infusion of lactic acid, the enterocytes are damaged and increased exfoliation results. A similar result is obtained in the blind-loop syndrome, where the accumulation of bile acids and bacteria provides the stress responsible for the destruction of the enterocytes, and in non-tropical spure, where the epithelium is attacked by noxious peptides in the diet. The first consequence of the accelerated desquamation is epithelial hyperplasia without zonal transformation, though the enterocytes are damaged - in contrast to those of the resected intestine - and apparently possess reduced enzyme activities. If the application of the stress is continued, a stage is reached in which the cell proliferation does not suffice to counteract the cell loss at the villus tips. Then a zonal transformation occurs whereby, despite the lengthened crypts, the villi become shorter and wider until, in extreme cases, the mucosa is completely devoid of villi. The transport capacity in vitro and in vivo - even when expressed in terms of unit-length is reduced, and good correlations exist between the reduction in function and the diminution in surface area of the intestine. In a self-emptying blind loop, the intestine is devoid of all contact with nutritional material. Under these circumstances, hypoplasia of the mucosa develops in which the enterocytes appear unchanged, though in extreme cases they may possess reduced enzyme levels, and the absorption capacity in vivo is consequently reduced.", "contents": "[Adaptive changes in intestinal mucosa of the small intestine as a result of intraluminal stress]. Under physiological conditions, the intestinal mucosa is the site of a delicate balance between cell proliferation in the crypt region and cell desquamation at the villus tips. This balance can be deranged by a number of endogenous or exogenous factors, oneof which is the intraluminal contents. This review discusses the effects of different modifications of the luminal milieu on the structure and function of the mucosa. Following intestinal resection or loop transposition, the contents reaching the remnant or the transposed loop differ markedly from those with which they come into contact under normal conditions. Hyperplasia without zonal transformation then develops in the experimental loop. The villi do not become wider, and changes in the strucutre of the epithelium are not observed. There are conflicting resutls in the literature concerning the enzyme activities in the individual enterocytes resulting from this hyperplasia. However, the epithelium its functionally immature, since transport capacities measured in vitro are reduced. On the other hand, the hyperplasia of the mucosa is such that absorptive capacities in vivo, when expressed in terms of intestinal length, are larger than normal. When the intestine is subjected to prolonged infusion of lactic acid, the enterocytes are damaged and increased exfoliation results. A similar result is obtained in the blind-loop syndrome, where the accumulation of bile acids and bacteria provides the stress responsible for the destruction of the enterocytes, and in non-tropical spure, where the epithelium is attacked by noxious peptides in the diet. The first consequence of the accelerated desquamation is epithelial hyperplasia without zonal transformation, though the enterocytes are damaged - in contrast to those of the resected intestine - and apparently possess reduced enzyme activities. If the application of the stress is continued, a stage is reached in which the cell proliferation does not suffice to counteract the cell loss at the villus tips. Then a zonal transformation occurs whereby, despite the lengthened crypts, the villi become shorter and wider until, in extreme cases, the mucosa is completely devoid of villi. The transport capacity in vitro and in vivo - even when expressed in terms of unit-length is reduced, and good correlations exist between the reduction in function and the diminution in surface area of the intestine. In a self-emptying blind loop, the intestine is devoid of all contact with nutritional material. Under these circumstances, hypoplasia of the mucosa develops in which the enterocytes appear unchanged, though in extreme cases they may possess reduced enzyme levels, and the absorption capacity in vivo is consequently reduced.", "PMID": 969785}
{"id": "pubmed23n0032_7636", "title": "[Corelations between thrombocyte count, clinical diagnosis, and liver pathology in chronic liver diseases].", "content": "In 1488 cases of chronic liver disease thrombocyte content of peripheral blood, clinical diagnosis and histologic findings of the liver biopsy were correlated. Ranging from fatty infiltration to fatty cirrhosis or from slightly active chronic hepatitis to active postnecrotic cirrhosis, according to the extent of the liver injury a significant decrease of thrombocyte-count was evaluated. In case of non-active liver disease and posthepatitic status a thrombocytopenia also could be found. Cirrhotic transformation or proliferation of the connective tissue mainly in the periportal fields and inflammatory activity showed a significant correlation to the frequency of a thrombocyte decrease. A pathogenetic relation is ascribed to an increased thrombocyte storage in the spleen depending on the extent of the liver injury and the alteration of the portal blood stream up to a portal hypertension. Our results indicate such correlations of liver injury, portal pressure and platelet-pooling to thrombocyte-count even if the causal dependency cannot be proved.", "contents": "[Corelations between thrombocyte count, clinical diagnosis, and liver pathology in chronic liver diseases]. In 1488 cases of chronic liver disease thrombocyte content of peripheral blood, clinical diagnosis and histologic findings of the liver biopsy were correlated. Ranging from fatty infiltration to fatty cirrhosis or from slightly active chronic hepatitis to active postnecrotic cirrhosis, according to the extent of the liver injury a significant decrease of thrombocyte-count was evaluated. In case of non-active liver disease and posthepatitic status a thrombocytopenia also could be found. Cirrhotic transformation or proliferation of the connective tissue mainly in the periportal fields and inflammatory activity showed a significant correlation to the frequency of a thrombocyte decrease. A pathogenetic relation is ascribed to an increased thrombocyte storage in the spleen depending on the extent of the liver injury and the alteration of the portal blood stream up to a portal hypertension. Our results indicate such correlations of liver injury, portal pressure and platelet-pooling to thrombocyte-count even if the causal dependency cannot be proved.", "PMID": 969786}
{"id": "pubmed23n0032_7637", "title": "[Stomach motility and lyspafen].", "content": "Studies of motility of the stomach in 10 test-persons resulted in a significant reduction of motility after a 2-day period of perorally intake of Lyspafen. The active motoric substance is diffenoxin-hydrochloride. An addition of 0,025 mg Atropin sulfate could increase the effect. The effect is independent of the clinical diagnose. A \"runaway\" could be explained by an intolerance against lactose. The waves of type I are less influenced, such as the frequency of the waves of contraction.", "contents": "[Stomach motility and lyspafen]. Studies of motility of the stomach in 10 test-persons resulted in a significant reduction of motility after a 2-day period of perorally intake of Lyspafen. The active motoric substance is diffenoxin-hydrochloride. An addition of 0,025 mg Atropin sulfate could increase the effect. The effect is independent of the clinical diagnose. A \"runaway\" could be explained by an intolerance against lactose. The waves of type I are less influenced, such as the frequency of the waves of contraction.", "PMID": 969787}
{"id": "pubmed23n0032_7638", "title": "[Acute pancreatitis in Mycoplasma pneumoniae infections].", "content": "Report on a man of 29 years with acute bronchopneumonia, antibodies against Mycoplasma pneumoniae and acute pancreatitis lasting three weeks. In some cases M. p. must be considered to be the cause of acute pancreatitis.", "contents": "[Acute pancreatitis in Mycoplasma pneumoniae infections]. Report on a man of 29 years with acute bronchopneumonia, antibodies against Mycoplasma pneumoniae and acute pancreatitis lasting three weeks. In some cases M. p. must be considered to be the cause of acute pancreatitis.", "PMID": 969788}
{"id": "pubmed23n0032_7639", "title": "[14CO2 exhalation tests. Diagnostic improvement in gastroenterologic diseases].", "content": "For evaluation of 14CO2-breath-tests the three most employed tests, namely glycero-14C-tripalmitate-test, 14C-lactose-tolerance-test, and 14C-glycin-cholate-test, were performed in healthy volunteers (n = 69), patients with chronic pancreatitis (n = 18), manifest malassimilation (n = 8), lactase deficiency (n = 15), and patients, in whom a disturbed enterohepatic bile salt circulation was suspected (n = 19). Usefulness of malabsorption tests was limited by many false normal results. Cholylglycin-breath-test on the other hand was sensitive, but clinical significance remained questionable. In our opinion simple performance and lacking discomfort are no sufficient arguments for 14CO2-breath-test.", "contents": "[14CO2 exhalation tests. Diagnostic improvement in gastroenterologic diseases]. For evaluation of 14CO2-breath-tests the three most employed tests, namely glycero-14C-tripalmitate-test, 14C-lactose-tolerance-test, and 14C-glycin-cholate-test, were performed in healthy volunteers (n = 69), patients with chronic pancreatitis (n = 18), manifest malassimilation (n = 8), lactase deficiency (n = 15), and patients, in whom a disturbed enterohepatic bile salt circulation was suspected (n = 19). Usefulness of malabsorption tests was limited by many false normal results. Cholylglycin-breath-test on the other hand was sensitive, but clinical significance remained questionable. In our opinion simple performance and lacking discomfort are no sufficient arguments for 14CO2-breath-test.", "PMID": 969789}
{"id": "pubmed23n0032_7640", "title": "[Determination of gestational age by sonar (author's transl)].", "content": "For some time it has been recognized that abnormal intrauterine development can lead to dissociation between maturity and external size and/or weight of the fetus. The results of pathophysiological and biochemical studies of fetal growth and nutrition have resulted in attempts to monitor and time every pregnancy at risk. The most recent information in the area of intrauterine development has shown that also organic and functional maturity of fetal organ systems can develop dissociated from gestational age. This observation points out the necessity for additional measures in order to achieve the goals of Planed obstetrics. The possibilities of ultrasound biometry for determining gestational age were presented. Taking normal and abnormal fetal growth and behavior into consideration, the use of ultrasonic diagnostic methods makes it possible to acquire concrete data regarding the size and maturity of some fetal organ systems. The methodological problems such as arise in clinical practice were explained and discussed in light of the most recent developments in equipment.", "contents": "[Determination of gestational age by sonar (author's transl)]. For some time it has been recognized that abnormal intrauterine development can lead to dissociation between maturity and external size and/or weight of the fetus. The results of pathophysiological and biochemical studies of fetal growth and nutrition have resulted in attempts to monitor and time every pregnancy at risk. The most recent information in the area of intrauterine development has shown that also organic and functional maturity of fetal organ systems can develop dissociated from gestational age. This observation points out the necessity for additional measures in order to achieve the goals of Planed obstetrics. The possibilities of ultrasound biometry for determining gestational age were presented. Taking normal and abnormal fetal growth and behavior into consideration, the use of ultrasonic diagnostic methods makes it possible to acquire concrete data regarding the size and maturity of some fetal organ systems. The methodological problems such as arise in clinical practice were explained and discussed in light of the most recent developments in equipment.", "PMID": 969790}
{"id": "pubmed23n0032_7641", "title": "[Topography of a reference plane for ultrasonic thoracometry (author's transl)].", "content": "A method of ultrasonic thoracometry is reported which uses the veins of the fetal liver as landmarks. The fact that the umbilical vein running from the anterior abdominal wall to the \"sinus venae portae\" is visible in B-scan display is the given presupposition. In a study about topography of the vein system of the fetal liver in 50 cases the description of a so called \"sinus-plane\" for sonar thoracometry is given. This reference plane is shown to be identical with the \"lower apertura of the fetal thorax\" recommended by Hansmann and co-workers since 1971. Thereby the nowadays well established method of thoracometry in obstetrical routine work gets a more accurate definition in regard to it's topography. This is not only of theoretical interest but will improve the conditions for more accuracy and reproducibility of the method.", "contents": "[Topography of a reference plane for ultrasonic thoracometry (author's transl)]. A method of ultrasonic thoracometry is reported which uses the veins of the fetal liver as landmarks. The fact that the umbilical vein running from the anterior abdominal wall to the \"sinus venae portae\" is visible in B-scan display is the given presupposition. In a study about topography of the vein system of the fetal liver in 50 cases the description of a so called \"sinus-plane\" for sonar thoracometry is given. This reference plane is shown to be identical with the \"lower apertura of the fetal thorax\" recommended by Hansmann and co-workers since 1971. Thereby the nowadays well established method of thoracometry in obstetrical routine work gets a more accurate definition in regard to it's topography. This is not only of theoretical interest but will improve the conditions for more accuracy and reproducibility of the method.", "PMID": 969791}
{"id": "pubmed23n0032_7642", "title": "[A contribution to the weight determination of the fetus by combined kephalo- and thoracometry (author's transl)].", "content": "By comparison of the sectional area of the fetal head of the fronto-occipital level in the height of the biparietalic diameter and the cross sectional area of thorax in the level area of the ventrical it was tried to determine the exspected birth weight. A mean deviation of 8,9% was found. Below a birth weight of 2500 g the presented calculation is only valid to some extend as in these cases the dominance proved in fetal development grounds of the head of the fetus becomes apparent.", "contents": "[A contribution to the weight determination of the fetus by combined kephalo- and thoracometry (author's transl)]. By comparison of the sectional area of the fetal head of the fronto-occipital level in the height of the biparietalic diameter and the cross sectional area of thorax in the level area of the ventrical it was tried to determine the exspected birth weight. A mean deviation of 8,9% was found. Below a birth weight of 2500 g the presented calculation is only valid to some extend as in these cases the dominance proved in fetal development grounds of the head of the fetus becomes apparent.", "PMID": 969792}
{"id": "pubmed23n0032_7643", "title": "[Comparison of serum lipid concentrations in umbilical artery, umbilical vein and maternal vein blood (author's transl)].", "content": "Maternal serum lipids and lipids in umbilical artery and umbilical vein blood were examined immediately after delivery in order to relate maternal and fetal lipid metabolism. Total lipids, phospholipids, triglycerides, total cholesterol, cholesterol esters and free cholesterol were determined. The comparison of arterial and venous umbilical lipid levels showed a significantly higher concentration of phospholipids in the umbilical vein, indicating an enhanced flux of phospholipids from the placenta to the fetus. A positive correlation is established for the amounts of total lipids (p less than 0,05), triglycerides (p less than 0,01), phospholipids (p less than 0,05) and free cholesterol (p less than 0,005) between maternal and umbilical vein blood. It is suggested that free cholesterol passes the placental barrier unchanged. The other correlations are discussed as the expression of a factor influencing simultaneously the maternal and fetal lipid metabolism.", "contents": "[Comparison of serum lipid concentrations in umbilical artery, umbilical vein and maternal vein blood (author's transl)]. Maternal serum lipids and lipids in umbilical artery and umbilical vein blood were examined immediately after delivery in order to relate maternal and fetal lipid metabolism. Total lipids, phospholipids, triglycerides, total cholesterol, cholesterol esters and free cholesterol were determined. The comparison of arterial and venous umbilical lipid levels showed a significantly higher concentration of phospholipids in the umbilical vein, indicating an enhanced flux of phospholipids from the placenta to the fetus. A positive correlation is established for the amounts of total lipids (p less than 0,05), triglycerides (p less than 0,01), phospholipids (p less than 0,05) and free cholesterol (p less than 0,005) between maternal and umbilical vein blood. It is suggested that free cholesterol passes the placental barrier unchanged. The other correlations are discussed as the expression of a factor influencing simultaneously the maternal and fetal lipid metabolism.", "PMID": 969794}
{"id": "pubmed23n0032_7644", "title": "[Comparison of lipid concentrations in lung homogenates of newborns with and without hyaline membrane disease (author's transl)].", "content": "Quantitative and qualitative studies on lipids of the lungs were performed in babies who died during the perinatal period. The lungs were divided into organs with and without hyaline membranes respectively. Total lipids, phospholipids, neutral lipids, lecithins and sphingomyelins were determined. The fatty acid composition of total phospholipids and of lecithins is reported. A multivariate analysis of variance was used for the verification of significant differences in lipid content and in fatty acid composition with regard to the histological diagnosis. Taking at least two parameters (total phospholipids and C: 14-fatty acid or lecithin and C: 14-fatty acid into account, the two groups (lungs with and without hyaline membranes) were significantly different at the 5% level. The results are compared as well with the lipid content and composition of fatty acids of normal neonatal lung tissue as with observations regarding the lipids in amniotic fluid. The data obtained are discussed with regard to the development of lung maturity.", "contents": "[Comparison of lipid concentrations in lung homogenates of newborns with and without hyaline membrane disease (author's transl)]. Quantitative and qualitative studies on lipids of the lungs were performed in babies who died during the perinatal period. The lungs were divided into organs with and without hyaline membranes respectively. Total lipids, phospholipids, neutral lipids, lecithins and sphingomyelins were determined. The fatty acid composition of total phospholipids and of lecithins is reported. A multivariate analysis of variance was used for the verification of significant differences in lipid content and in fatty acid composition with regard to the histological diagnosis. Taking at least two parameters (total phospholipids and C: 14-fatty acid or lecithin and C: 14-fatty acid into account, the two groups (lungs with and without hyaline membranes) were significantly different at the 5% level. The results are compared as well with the lipid content and composition of fatty acids of normal neonatal lung tissue as with observations regarding the lipids in amniotic fluid. The data obtained are discussed with regard to the development of lung maturity.", "PMID": 969793}
{"id": "pubmed23n0032_7645", "title": "[Lipid composition in placental homogenates of mature, premature and small for gestational age newborns (author's transl)].", "content": "Total lipids, phospholipids, and neutral lipids were determined in the placentas of mature, premature and small for gestational age babies. In addition the phospholipids and neutral lipids were calculated as percentage of total lipids. The distribution of fatty acids in placental phospholipids is reported. The difference found in the concentration of placental lipids is statistically significant at the 5% level between the groups of premature and small for gestational age babies. The increase of neutral lipids and the reduced content of phospholipids in placentas of small for gestational age babies are discussed as a possible sign of placental insufficiency.", "contents": "[Lipid composition in placental homogenates of mature, premature and small for gestational age newborns (author's transl)]. Total lipids, phospholipids, and neutral lipids were determined in the placentas of mature, premature and small for gestational age babies. In addition the phospholipids and neutral lipids were calculated as percentage of total lipids. The distribution of fatty acids in placental phospholipids is reported. The difference found in the concentration of placental lipids is statistically significant at the 5% level between the groups of premature and small for gestational age babies. The increase of neutral lipids and the reduced content of phospholipids in placentas of small for gestational age babies are discussed as a possible sign of placental insufficiency.", "PMID": 969795}
{"id": "pubmed23n0032_7646", "title": "[Influence of allylestrenol on hpl serum levels in high-risk pregnancies (author's transl)].", "content": "32 patients with high-risk pregnancies were treated with 3 X 5 mg of 17alpha-ally1-17beta-hydroxy-estr-4-ene (allylestrenol, Gestanon) daily. Only those patients entered this study, who had pathological HPL serum levels during two successive control investigations. In addition, cytological analyses of vaginal smears were performed before treatment was started. The patients were treated with allylestrenol until normal HPL levels were recorded, but at least during 14 days. HPL serum levels were estimated every second day through 14 days. Thereafter HPL assays were performed in weekly intervals. Determinations of HPL were done by radioimmunoassay. Analyses of vaginal smears were done weekly. Statistical analysis of HPL serum levels were performed by Student's-t-test. A statistical significant increase of HPL serum levels were registered during allylestrenol therapy. These results suggest that the pregnancy maintaining effect of allylestrenol is at least partly due to a stimulation of placental function. Analyses of vaginal smears indicate a progestational effect of the norsteroid.", "contents": "[Influence of allylestrenol on hpl serum levels in high-risk pregnancies (author's transl)]. 32 patients with high-risk pregnancies were treated with 3 X 5 mg of 17alpha-ally1-17beta-hydroxy-estr-4-ene (allylestrenol, Gestanon) daily. Only those patients entered this study, who had pathological HPL serum levels during two successive control investigations. In addition, cytological analyses of vaginal smears were performed before treatment was started. The patients were treated with allylestrenol until normal HPL levels were recorded, but at least during 14 days. HPL serum levels were estimated every second day through 14 days. Thereafter HPL assays were performed in weekly intervals. Determinations of HPL were done by radioimmunoassay. Analyses of vaginal smears were done weekly. Statistical analysis of HPL serum levels were performed by Student's-t-test. A statistical significant increase of HPL serum levels were registered during allylestrenol therapy. These results suggest that the pregnancy maintaining effect of allylestrenol is at least partly due to a stimulation of placental function. Analyses of vaginal smears indicate a progestational effect of the norsteroid.", "PMID": 969796}
{"id": "pubmed23n0032_7647", "title": "Largactil (chlorpromasine) and cervical dilatation in primiparae.", "content": "The effects of Largactil on cervical maturation and dilatation during induced labour in primiparous women were investigated. The obtained results suggest that Largactil has significant influence on the maturation and dilatation of the cervix in severely \"neurotic\" women relative to \"non-neurotic\" women.", "contents": "Largactil (chlorpromasine) and cervical dilatation in primiparae. The effects of Largactil on cervical maturation and dilatation during induced labour in primiparous women were investigated. The obtained results suggest that Largactil has significant influence on the maturation and dilatation of the cervix in severely \"neurotic\" women relative to \"non-neurotic\" women.", "PMID": 969797}
{"id": "pubmed23n0032_7648", "title": "[Changes in maternal cardiovascular parameter during tocolysis and in the dorsal position shock syndrome (author's transl)].", "content": "Maternal circulatory parameters and fetal heart rate were measured in 25 healthy pregnant women in the last trimenon during treatment with Fenoterol, Fenoterol in combination with Verapamil and Verapamil alone. Dosages were used in accordance with the tocolytic guidelines from Weidinger and Wiest. We were able to demonstrate that the betamimetic Fenoterol alone and in combination with the Ca++-antagonist Verapamil strongly increases the maternal heart rate an the maternal cardiac output whereas the peripheral resistance decreases accordingly. The average blood pressure stayed leveled, so that a decreased uterine blood flow cannot be assumed under betamimetics from the maternal cardiovascular point of view. However, there are indications for an increased placental blood flow during tocolysis. The betamimetic drug show no significant effect on the fetal heart rate. Additional application of the Ca++-antagonist Verapamil during tocolysis with Fenoterol (in dosages usually used for tocolysis) doesn't change cardiovascular reactions caused by Fenoterol. Change in position from supine to left lateral position caused a short term increase in the maternal cardiac output even noted in pregnant women without a clinically observed cavasyndrom. These changes of maternal cardiac output are comparable with those in orthostatic stress situations.", "contents": "[Changes in maternal cardiovascular parameter during tocolysis and in the dorsal position shock syndrome (author's transl)]. Maternal circulatory parameters and fetal heart rate were measured in 25 healthy pregnant women in the last trimenon during treatment with Fenoterol, Fenoterol in combination with Verapamil and Verapamil alone. Dosages were used in accordance with the tocolytic guidelines from Weidinger and Wiest. We were able to demonstrate that the betamimetic Fenoterol alone and in combination with the Ca++-antagonist Verapamil strongly increases the maternal heart rate an the maternal cardiac output whereas the peripheral resistance decreases accordingly. The average blood pressure stayed leveled, so that a decreased uterine blood flow cannot be assumed under betamimetics from the maternal cardiovascular point of view. However, there are indications for an increased placental blood flow during tocolysis. The betamimetic drug show no significant effect on the fetal heart rate. Additional application of the Ca++-antagonist Verapamil during tocolysis with Fenoterol (in dosages usually used for tocolysis) doesn't change cardiovascular reactions caused by Fenoterol. Change in position from supine to left lateral position caused a short term increase in the maternal cardiac output even noted in pregnant women without a clinically observed cavasyndrom. These changes of maternal cardiac output are comparable with those in orthostatic stress situations.", "PMID": 969798}
{"id": "pubmed23n0032_7649", "title": "[Clinical research on a new corticoid-containing topical drug].", "content": "In the present paper, the clinical trial of a new difluorinated corticosteroid suitable for topical use is reported. Because of its proven efficacy in very different dermatoses, it is useful as a new reliable product for external use having a broad spectrum of indications. Altogether, 50 patients were treated for a number of dermatoses (various forms of eczema, neurodermitis constitutionalis among other rare skin diseases). Complications, especially skin lesions due to corticosteroids, were not observed. The availability of the test preparation in three different forms has the advantage that it is not only suitable for the primary dermatosis, but it can also be adapted to the various degrees of acuteness.", "contents": "[Clinical research on a new corticoid-containing topical drug]. In the present paper, the clinical trial of a new difluorinated corticosteroid suitable for topical use is reported. Because of its proven efficacy in very different dermatoses, it is useful as a new reliable product for external use having a broad spectrum of indications. Altogether, 50 patients were treated for a number of dermatoses (various forms of eczema, neurodermitis constitutionalis among other rare skin diseases). Complications, especially skin lesions due to corticosteroids, were not observed. The availability of the test preparation in three different forms has the advantage that it is not only suitable for the primary dermatosis, but it can also be adapted to the various degrees of acuteness.", "PMID": 969808}
{"id": "pubmed23n0032_7650", "title": "[Echocardiographic evaluation of left ventricular function during therapy with cardiovascularly effective drugs].", "content": "The task of this study was to examine the feasibility of applying echocardiography in determining alterations of left ventricular performance during administration of vasoactive drugs. Patients suffering from congestive heart failure were treated with Digitalis, other patients with normal hearts were subjected to infusion therapy of Isoproterenol, Propranolol, Verapamil and Calcium chloride. A new concept for interpretation of function curves relating the velocity of circumferential fiber shortening (VCF) and time was introduced to establish the inotropic, bathmotropic and chronotropic effects of vasoactive drugs to the myocardium. It can be concluded that echocardiography is a feasible tool in determining changes of left ventricular dynamics during administration of vasoactive drugs, in indicating the individual dosage of a vasoactive drug in each patient and the follow-up the course of the therapeutic success.", "contents": "[Echocardiographic evaluation of left ventricular function during therapy with cardiovascularly effective drugs]. The task of this study was to examine the feasibility of applying echocardiography in determining alterations of left ventricular performance during administration of vasoactive drugs. Patients suffering from congestive heart failure were treated with Digitalis, other patients with normal hearts were subjected to infusion therapy of Isoproterenol, Propranolol, Verapamil and Calcium chloride. A new concept for interpretation of function curves relating the velocity of circumferential fiber shortening (VCF) and time was introduced to establish the inotropic, bathmotropic and chronotropic effects of vasoactive drugs to the myocardium. It can be concluded that echocardiography is a feasible tool in determining changes of left ventricular dynamics during administration of vasoactive drugs, in indicating the individual dosage of a vasoactive drug in each patient and the follow-up the course of the therapeutic success.", "PMID": 969820}
{"id": "pubmed23n0032_7651", "title": "[Angiography of left ventricle following volume load and physical work in coronary disease].", "content": "In order to recognize and quantitate abnormalities in wall segment motion in 22 patients simultaneous measurements of isovolumic and ejection phase parameters were performed with the Millar-angiographic catheter at rest, after leg raising and during bicycle exercise. 6 patients had slight coronary heart disease (CHD) (group I), 16 patients had severe CHD (group II). During volume load in all patients of gr. II a decrease of peak measured velocity (Vpm) and the velocity of circumferential fiber shortening (Vcf from 1.4 to 1.1 circ/s, p less than 0.025) occurred. In gr. I Vcf increased (p less than 0.05). During exercise there was a high increase in Vpm and Vcf in gr. I (from 1.2 to 1.9 circ/s) whereas in gr. II no increase was observed. LVEDP rose to 29 mm Hg and ESV from 58 to 70 ml/1.73 m2. By increasing LVEDP during volume load underperfused areas became ischaemic and akinetic. During physical work the segmental abnormality can be less pronounced possibly due to the higher prestenotic pressure.", "contents": "[Angiography of left ventricle following volume load and physical work in coronary disease]. In order to recognize and quantitate abnormalities in wall segment motion in 22 patients simultaneous measurements of isovolumic and ejection phase parameters were performed with the Millar-angiographic catheter at rest, after leg raising and during bicycle exercise. 6 patients had slight coronary heart disease (CHD) (group I), 16 patients had severe CHD (group II). During volume load in all patients of gr. II a decrease of peak measured velocity (Vpm) and the velocity of circumferential fiber shortening (Vcf from 1.4 to 1.1 circ/s, p less than 0.025) occurred. In gr. I Vcf increased (p less than 0.05). During exercise there was a high increase in Vpm and Vcf in gr. I (from 1.2 to 1.9 circ/s) whereas in gr. II no increase was observed. LVEDP rose to 29 mm Hg and ESV from 58 to 70 ml/1.73 m2. By increasing LVEDP during volume load underperfused areas became ischaemic and akinetic. During physical work the segmental abnormality can be less pronounced possibly due to the higher prestenotic pressure.", "PMID": 969821}
{"id": "pubmed23n0032_7652", "title": "[Systolic time intervals of the left ventricle in neuromuscular diseases].", "content": "In 64 patients having neuromuscular disease and in 11 healthy persons, preejection period (PEP), ejection time index (LVETI) and PEP/LVET ratio of the left ventricle were studied by indirect method. PEP was significantly longer, ejection time index was significantly shorter and PEP/LVET ratio was significantly higher in patients with progressive muscular dystrophy than healthy persons. In 75% of patients having this disease LVETI was shortened and only in 30% of them Ecg abnormalities could be detected. These findings are postulated to be due to diffusely decreased contractility of the left ventricular heart muscle in progressive muscular dystrophy.", "contents": "[Systolic time intervals of the left ventricle in neuromuscular diseases]. In 64 patients having neuromuscular disease and in 11 healthy persons, preejection period (PEP), ejection time index (LVETI) and PEP/LVET ratio of the left ventricle were studied by indirect method. PEP was significantly longer, ejection time index was significantly shorter and PEP/LVET ratio was significantly higher in patients with progressive muscular dystrophy than healthy persons. In 75% of patients having this disease LVETI was shortened and only in 30% of them Ecg abnormalities could be detected. These findings are postulated to be due to diffusely decreased contractility of the left ventricular heart muscle in progressive muscular dystrophy.", "PMID": 969822}
{"id": "pubmed23n0032_7653", "title": "[Iron deficiency following implantation of a heart valve prosthesis].", "content": "In 44 patients with single or multiple valvular protheses of the heart, the problem of secondary iron deficiency due to mechanical hemolysis was investigated. In 17 cases, elevated serum transferrin values were noted, compared to elevated serum iron levels in only 6 cases. Therefore, treatment should be based on the more reliable transferrin values. Severity and duration of mechanical hemolysis did not seem to be correlated with the occurrence or the degree of iron deficiency.", "contents": "[Iron deficiency following implantation of a heart valve prosthesis]. In 44 patients with single or multiple valvular protheses of the heart, the problem of secondary iron deficiency due to mechanical hemolysis was investigated. In 17 cases, elevated serum transferrin values were noted, compared to elevated serum iron levels in only 6 cases. Therefore, treatment should be based on the more reliable transferrin values. Severity and duration of mechanical hemolysis did not seem to be correlated with the occurrence or the degree of iron deficiency.", "PMID": 969823}
{"id": "pubmed23n0032_7654", "title": "[Pulmonary valve aplasia with ventricular septal defect. Hemodynamics and embryology].", "content": "The combination of absent pulmonary valve with ventricular septal defect, subvalvular pulmonic stenosis and aneurysm of the pulmonary artery presents a characteristic heamodynamic and clinical profile. It should be differentiated from other morphologically related cardiovascular malformations. According to the degree of right ventricular outflow tract obstruction, the circulatory pattern and the clinical picture is varying widely. We are differentiating an obstructive and a congestive form of the disease. An own case is demonstrated. After a short review of the literature the clinical and haemodynamic findings are discussed. The important role of the ductus arteriosus in fetal circulation is emphasized. The development of a huge pulmonary aneurysm and the premature closing of the ductus arteriosus are considered to be secondary to the serious disturbance of fetal haemodynamics.", "contents": "[Pulmonary valve aplasia with ventricular septal defect. Hemodynamics and embryology]. The combination of absent pulmonary valve with ventricular septal defect, subvalvular pulmonic stenosis and aneurysm of the pulmonary artery presents a characteristic heamodynamic and clinical profile. It should be differentiated from other morphologically related cardiovascular malformations. According to the degree of right ventricular outflow tract obstruction, the circulatory pattern and the clinical picture is varying widely. We are differentiating an obstructive and a congestive form of the disease. An own case is demonstrated. After a short review of the literature the clinical and haemodynamic findings are discussed. The important role of the ductus arteriosus in fetal circulation is emphasized. The development of a huge pulmonary aneurysm and the premature closing of the ductus arteriosus are considered to be secondary to the serious disturbance of fetal haemodynamics.", "PMID": 969824}
{"id": "pubmed23n0032_7655", "title": "[Methodical aspects of pneumatic segment plethysmography. III. Measuring tourniquet problems].", "content": "The question, to which extent utilization of different plethysmographic cuffs influenced the assessment of volume changes, was examined in 40 healthy individuals as well as by means of an experimental model. It could be shown that plethysmographic results varied significantly according to the design of the cuffs: 1.a) Utilization of short (19.5 cm) cuffs that did not cover the entire thigh circumference resulted in significantly lower arterial inflow and pressure-dependent venous capacity measurements as compared to long cuffs (48.8 cm). b) This result was confirmed by use of a newly developed \"calibration-cylinder\" demonstrating that use of the short cuff showed only 62.2% of the true volume change, use of the long cuff, however, 89.6%. Deficiency of thigh contact is discussed as possible etiologic factor for this difference. 2. The cover material of the cuff also influenced the results significantly since results could be improved from 62.2% to 86.9% (short cuff) and from 89.6% to 95.1% (long cuff) by use of double layer non-compliant cloth. The reason for this finding is thought to be the compliance of conventional cloth. 3. Comparison of two cuffs with identical shape and dimensions resulted in significantly lower volume changes using hard stiff rubber as compared to soft compliant rubber. These findings suggest some recommendations for optimal design of plethysmographic cuffs. Cuffs should not underestimate volume changes obtained with the \"calibration-cylinder\" by more than 10%.", "contents": "[Methodical aspects of pneumatic segment plethysmography. III. Measuring tourniquet problems]. The question, to which extent utilization of different plethysmographic cuffs influenced the assessment of volume changes, was examined in 40 healthy individuals as well as by means of an experimental model. It could be shown that plethysmographic results varied significantly according to the design of the cuffs: 1.a) Utilization of short (19.5 cm) cuffs that did not cover the entire thigh circumference resulted in significantly lower arterial inflow and pressure-dependent venous capacity measurements as compared to long cuffs (48.8 cm). b) This result was confirmed by use of a newly developed \"calibration-cylinder\" demonstrating that use of the short cuff showed only 62.2% of the true volume change, use of the long cuff, however, 89.6%. Deficiency of thigh contact is discussed as possible etiologic factor for this difference. 2. The cover material of the cuff also influenced the results significantly since results could be improved from 62.2% to 86.9% (short cuff) and from 89.6% to 95.1% (long cuff) by use of double layer non-compliant cloth. The reason for this finding is thought to be the compliance of conventional cloth. 3. Comparison of two cuffs with identical shape and dimensions resulted in significantly lower volume changes using hard stiff rubber as compared to soft compliant rubber. These findings suggest some recommendations for optimal design of plethysmographic cuffs. Cuffs should not underestimate volume changes obtained with the \"calibration-cylinder\" by more than 10%.", "PMID": 969825}
{"id": "pubmed23n0032_7656", "title": "[Influence of atrial contraction on stroke volume in pacemaker stimulation].", "content": "The contribution of the atrial systole to the stroke volume was studied in 12 patients with implanted pacemakers by means of impedance-cardiography. P-ST-intervals between 70 and 200 msec. increased the stroke volume as compared with systoles without preceding P-waves. A maximal stroke volume was observed with a P-ST-interval of 130 msec. The increase of the stroke volume by 33% justifies the application of atrial triggered pacemakers in selected cases, mainly in younger patients despite of the technical limitations of these pacemaker systems.", "contents": "[Influence of atrial contraction on stroke volume in pacemaker stimulation]. The contribution of the atrial systole to the stroke volume was studied in 12 patients with implanted pacemakers by means of impedance-cardiography. P-ST-intervals between 70 and 200 msec. increased the stroke volume as compared with systoles without preceding P-waves. A maximal stroke volume was observed with a P-ST-interval of 130 msec. The increase of the stroke volume by 33% justifies the application of atrial triggered pacemakers in selected cases, mainly in younger patients despite of the technical limitations of these pacemaker systems.", "PMID": 969826}
{"id": "pubmed23n0032_7657", "title": "Oesophageal phlebectasis in an infant with pulmonary venous obstruction owing to a congenital heart defect.", "content": "Clinical findings and postmortem examination of a newborn infant are reported. Prominent features were a complex congenital heart defect (\"pre-mature closure of foramen ovale\", with mitral and aortic atresia), causing severe obstruction to pulmonary venous return, pulmonary lymphangiectasis and oesophageal phlebectasis. The occurrence of oesophageal phlebectasis in infants with congenital pulmonary venous obstruction is reviewed and the pathogenesis discussed.", "contents": "Oesophageal phlebectasis in an infant with pulmonary venous obstruction owing to a congenital heart defect. Clinical findings and postmortem examination of a newborn infant are reported. Prominent features were a complex congenital heart defect (\"pre-mature closure of foramen ovale\", with mitral and aortic atresia), causing severe obstruction to pulmonary venous return, pulmonary lymphangiectasis and oesophageal phlebectasis. The occurrence of oesophageal phlebectasis in infants with congenital pulmonary venous obstruction is reviewed and the pathogenesis discussed.", "PMID": 969827}
{"id": "pubmed23n0032_7658", "title": "[T inversion in Friedreich's ataxia].", "content": "The electrocardiogram of Friedrich's disease shows pathological findings in all examined patients, mostly T-wave inversion. On the other hand cases of abortive Friedreich's disease and of cerebellar ataxia Nonne-Marie don't present any substantial alteration of ecg. To a minor extent roentgenological findings might suggest Friedreich's cardiomyopathy discussed as a possible result of a neuro-myo-cardiac process.", "contents": "[T inversion in Friedreich's ataxia]. The electrocardiogram of Friedrich's disease shows pathological findings in all examined patients, mostly T-wave inversion. On the other hand cases of abortive Friedreich's disease and of cerebellar ataxia Nonne-Marie don't present any substantial alteration of ecg. To a minor extent roentgenological findings might suggest Friedreich's cardiomyopathy discussed as a possible result of a neuro-myo-cardiac process.", "PMID": 969828}
{"id": "pubmed23n0032_7659", "title": "[Time serial study of orthostatic cardiovascular regulations].", "content": "In a repeated measurement design (16 times during 8 weeks) active orthostasis was investigated in a sample of twenty male students, utilizing a tilt table. Additional data included cardiovascular and pulmonary tests, psychological scales, items from a diary and meterorological data. Analysis of orthostatic time-series was carried out with hierarchical grouping analysis for heart rate and blood pressure. Results indicated three intraindividual patterns of orthostatic reaction called normo-, hyper- and hyporeactive. These patterns are independent of the known interindividual orthostatic reaction types. Analysis of variance of the described patterns revealed differences for physiological and meteorological data and some items of the diary. These results have been crossvalidated.", "contents": "[Time serial study of orthostatic cardiovascular regulations]. In a repeated measurement design (16 times during 8 weeks) active orthostasis was investigated in a sample of twenty male students, utilizing a tilt table. Additional data included cardiovascular and pulmonary tests, psychological scales, items from a diary and meterorological data. Analysis of orthostatic time-series was carried out with hierarchical grouping analysis for heart rate and blood pressure. Results indicated three intraindividual patterns of orthostatic reaction called normo-, hyper- and hyporeactive. These patterns are independent of the known interindividual orthostatic reaction types. Analysis of variance of the described patterns revealed differences for physiological and meteorological data and some items of the diary. These results have been crossvalidated.", "PMID": 969829}
{"id": "pubmed23n0032_7660", "title": "[Fast fluorimetric assay of chlortetracycline in meat and bone (author's transl)].", "content": "Antibiotics in biological materials, including tetracyclines, are determined usually be means of microbiological methods. Because of the low specificity and the time consuming procedure of the microbiological assays, we developed a fluorimetric method, which enables us to determine specifically the tetracyclines and to reduce the time for the assay down to 4 hours. Our method is based on the determination of tetracyclines published by Kohn (1961), which we modified for the determination of chlortetracycline in meat and bone. Tetracyclines are fluorescent compounds, whose quantum yields are very much enhanced in most cases by forming a complex with Ca2+ ions and by extracting it into an organic solvent. This transfer into acetic acid ethylester separates the chlortetracycline from interfering substances. The yield of chlortetracycline with the described method was about 80%, the sensitivity of the detection was about 0.5 ppm.", "contents": "[Fast fluorimetric assay of chlortetracycline in meat and bone (author's transl)]. Antibiotics in biological materials, including tetracyclines, are determined usually be means of microbiological methods. Because of the low specificity and the time consuming procedure of the microbiological assays, we developed a fluorimetric method, which enables us to determine specifically the tetracyclines and to reduce the time for the assay down to 4 hours. Our method is based on the determination of tetracyclines published by Kohn (1961), which we modified for the determination of chlortetracycline in meat and bone. Tetracyclines are fluorescent compounds, whose quantum yields are very much enhanced in most cases by forming a complex with Ca2+ ions and by extracting it into an organic solvent. This transfer into acetic acid ethylester separates the chlortetracycline from interfering substances. The yield of chlortetracycline with the described method was about 80%, the sensitivity of the detection was about 0.5 ppm.", "PMID": 969905}
{"id": "pubmed23n0032_7661", "title": "[Uptake of mercury by plants (author's transl)].", "content": "The uptake of merkury by plants and animals in the immediate vicinity of a chlor-alkali plant is determined. Thereby it appeared that plants take up mercury from the air and accumulate it in assimilation tissue. Uptake and accumulation of mercury are manifested by in vivo experiments.", "contents": "[Uptake of mercury by plants (author's transl)]. The uptake of merkury by plants and animals in the immediate vicinity of a chlor-alkali plant is determined. Thereby it appeared that plants take up mercury from the air and accumulate it in assimilation tissue. Uptake and accumulation of mercury are manifested by in vivo experiments.", "PMID": 969906}
{"id": "pubmed23n0032_7662", "title": "[GLC-mass-spectrometrical investigation of the volatile components of wines vii. Aroma compounds of tokaj aszu wines b) organic acids (author's transl)].", "content": "An inventory of the organic acids detectable by liquid-liquid extraction has been made in different Tokaj wines. After the separation of the acidic compounds in the aroma extracts using Na2CO3 and after the derivatization with diazomethane 65 methylester have been separated by gaschromatography and identified by mass spectrometry. Using a special fractionation method organic acids, amino acids, carbohydrates and phenolic compounds have been isolated in different groups. After the derivatization of the components 50 organic acids have been quantitatively determined by gaschromatography. The compositions of the acids in the wines differ considerably. The quantitative differences are caused by the vintages, the method of production and the kind of storage. The possible biosynthetic pathways of the various acids are discussed.", "contents": "[GLC-mass-spectrometrical investigation of the volatile components of wines vii. Aroma compounds of tokaj aszu wines b) organic acids (author's transl)]. An inventory of the organic acids detectable by liquid-liquid extraction has been made in different Tokaj wines. After the separation of the acidic compounds in the aroma extracts using Na2CO3 and after the derivatization with diazomethane 65 methylester have been separated by gaschromatography and identified by mass spectrometry. Using a special fractionation method organic acids, amino acids, carbohydrates and phenolic compounds have been isolated in different groups. After the derivatization of the components 50 organic acids have been quantitatively determined by gaschromatography. The compositions of the acids in the wines differ considerably. The quantitative differences are caused by the vintages, the method of production and the kind of storage. The possible biosynthetic pathways of the various acids are discussed.", "PMID": 969907}
{"id": "pubmed23n0032_7663", "title": "[1H-NMR determation of ethanol in wine, winelike beverages, and liquors (author's transl)].", "content": "It has been demonstrated that a routinely employed quick test for the determination of ethanol in alcoholic beverages may be carried out with an accuracy of +/- 3% in the region of 0--80 Vol.-% ethanol content.", "contents": "[1H-NMR determation of ethanol in wine, winelike beverages, and liquors (author's transl)]. It has been demonstrated that a routinely employed quick test for the determination of ethanol in alcoholic beverages may be carried out with an accuracy of +/- 3% in the region of 0--80 Vol.-% ethanol content.", "PMID": 969908}
{"id": "pubmed23n0032_7664", "title": "[Analysis of polysaccharides used as food additives iv. Gaschromatographic identification and determination of neutral components in polysaccharide-hydrolysates (author's transl)].", "content": "The monosaccharides, which are obtained by hydrolytic cleavage of the polysaccharid containing stabilisators, can be separated gaschromatographically by the way of their aldonitrilacetates. After hydrolysis, the mixtures are neutralized by an anion exchanger, evaporated to dryness, converted in pyridin with hydroxylamin to the aldoximes and hereafter with acetic anhydride to the aldonitrilacetates. Dissolved in chloroform, these compounds are used for gaschromatographic separations; only one peak is obtained for each sugar. This is a great advantage to the previous methods, where the anomeric constellations lead up of five peaks for one sugar compound and for complicated sugar mixture, it therefore was difficult to evaluate their gaschromatograms.", "contents": "[Analysis of polysaccharides used as food additives iv. Gaschromatographic identification and determination of neutral components in polysaccharide-hydrolysates (author's transl)]. The monosaccharides, which are obtained by hydrolytic cleavage of the polysaccharid containing stabilisators, can be separated gaschromatographically by the way of their aldonitrilacetates. After hydrolysis, the mixtures are neutralized by an anion exchanger, evaporated to dryness, converted in pyridin with hydroxylamin to the aldoximes and hereafter with acetic anhydride to the aldonitrilacetates. Dissolved in chloroform, these compounds are used for gaschromatographic separations; only one peak is obtained for each sugar. This is a great advantage to the previous methods, where the anomeric constellations lead up of five peaks for one sugar compound and for complicated sugar mixture, it therefore was difficult to evaluate their gaschromatograms.", "PMID": 969909}
{"id": "pubmed23n0032_7665", "title": "[The automated kinetic determination of enzyme activities and constituents of plants. 3. Determination of bromelain (author's transl)].", "content": "With bromelain as protease model a kinetic method, capable of automatization, is described for the assay of proteolytic activities. The method uses milk protein as a substrate. The volume of the test mixture is fixed to 1 ml; the readings are performed at 340 nm. With the bromelain preparation used (2 mAnson-U/mg) a linear decline of O.D. (deltaA/min) between 5 and 50 mug bromelain/ml is observed. Other proteases as well as protease inhibitors may be analyzed by the same method also.", "contents": "[The automated kinetic determination of enzyme activities and constituents of plants. 3. Determination of bromelain (author's transl)]. With bromelain as protease model a kinetic method, capable of automatization, is described for the assay of proteolytic activities. The method uses milk protein as a substrate. The volume of the test mixture is fixed to 1 ml; the readings are performed at 340 nm. With the bromelain preparation used (2 mAnson-U/mg) a linear decline of O.D. (deltaA/min) between 5 and 50 mug bromelain/ml is observed. Other proteases as well as protease inhibitors may be analyzed by the same method also.", "PMID": 969910}
{"id": "pubmed23n0032_7666", "title": "[Acetosulfam, a new sweetener. 1. synthesis and properties (author's transl)].", "content": "A survey is given of synthesis, chemical and physical properties, and hydrolytic stability of the new sweetener Acetosulfam (3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one-2,2 dioxide potassium salt).", "contents": "[Acetosulfam, a new sweetener. 1. synthesis and properties (author's transl)]. A survey is given of synthesis, chemical and physical properties, and hydrolytic stability of the new sweetener Acetosulfam (3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one-2,2 dioxide potassium salt).", "PMID": 969911}
{"id": "pubmed23n0032_7667", "title": "A recording differential respirometer.", "content": "A recording differential respirometer capable of micromeasurement of gaseous changes is described. The rate of volume change under constant pressure is recorded directly. Due to the mechanical nature of the volume measurement, the instrument is drift-free. Factors affecting the sensitivity and correction for deviation due to change in total volumes are discussed.", "contents": "A recording differential respirometer. A recording differential respirometer capable of micromeasurement of gaseous changes is described. The rate of volume change under constant pressure is recorded directly. Due to the mechanical nature of the volume measurement, the instrument is drift-free. Factors affecting the sensitivity and correction for deviation due to change in total volumes are discussed.", "PMID": 969912}
{"id": "pubmed23n0032_7668", "title": "[Identification of irradiated onions (author's transl)].", "content": "A method is described by which irradiated onions are identified by failure to root formation.", "contents": "[Identification of irradiated onions (author's transl)]. A method is described by which irradiated onions are identified by failure to root formation.", "PMID": 969913}
{"id": "pubmed23n0032_7669", "title": "[Separation and quantitative \"in situ\"-determination of organotin compounds on thin layer plates].", "content": "Residues of organotin compounds in feed and food are extracted with isopropanol. Clean up by means of a Sephadex LH-20 column and separation of the compounds of Kieselgel-G thin layer plates by 4-methyl-pentanone (2)/pyridine/acetic acid (97.5 + 1.5 +1) is achieved. Triphenyl- and tricyclohexyltin fungicides are quantitatively determined by direct scanning the thin layer plates using a chromatogram spectralphotometer.", "contents": "[Separation and quantitative \"in situ\"-determination of organotin compounds on thin layer plates]. Residues of organotin compounds in feed and food are extracted with isopropanol. Clean up by means of a Sephadex LH-20 column and separation of the compounds of Kieselgel-G thin layer plates by 4-methyl-pentanone (2)/pyridine/acetic acid (97.5 + 1.5 +1) is achieved. Triphenyl- and tricyclohexyltin fungicides are quantitatively determined by direct scanning the thin layer plates using a chromatogram spectralphotometer.", "PMID": 969914}
{"id": "pubmed23n0032_7670", "title": "[Sample survey of smoking habits in the German, French and Romansch speaking parts of Switzerland in 1975].", "content": "In a survey of a representative sample of 900 persons in Switzerland (excluding the Italian speaking parts comprising some 4-5 percent of the population), the smoking habits of the adult population were studied. Smokers were defined as persons smoking at least one cigarette or an equivalent weight of pipe tobacco or cigars per day. 51.8% of men and 29.0% of women over age 15 are smokers. Among male smokers of cigarettes only (42% of all men), over three quarters (77.6%) smoke ten cigarettes or more per day, i.e., a quantity found harmful to health; over one half (55.8%) smoke twenty or more cigarettes per day. Half of all female smokers smoke ten or more cigarettes, more than one in every four female smokers (28.8%) smoke twenty or more cigarettes per day. It is calculated that almost two thirds of all cigarettes sold in Switzerland are smoked by smokers of twenty or more cigarettes per day, and that almost three quarters are smoked by smokers of ten or more cigarettes. Among men, smoking habits are independent of social status, whereas among women, those with higher family incomes show more frequent smoking, but also more widespread cessation of smoking than with lower incomes. Thirty percent of adults having been smokers have given up the habit, so that among men and women above age 15, theree are now some 20% and 14% respectively, of ex-smokers. This corresponds to at least 700,000 former smokers in all of Switzerland. The trend to give up starts already in the group aged 15-24 (over 20% of men and women having ever smoked have given up) and becomes more marked with increasing age. A large part of male and female smokers would like to free themselves of the habit. 30% of male and 34% of female smokers indicate that they had tried seriously to stop smoking during the twelve months preceding the interview. Trying to give up was not significantly associated with intensity of smoking or social status. According to this survey, a majority of the population (57.6%) is in favor of reserving certain parts of restaurants to non-smokers, following the example of non-smokers-compartments in railway trains. In a situation where a non-smoker asks a co-worker in a closed room not to smoke, 78.3% take sides with the non-smoker, either by conceding to him a right to smoke-free air, or by demanding from the smoker to show consideration for the non-smoker. Only 10.9% demand from the non-smoker understanding for the smoker, or concede to the smoker a right to smoke at work. Women show more understanding for cause of the non-smoker than men. This difference is more marked among smokers and former smokers than among non-smokers. Inasmuch as the results of this study are comparable to those of earlier surveys conducted in Switzerland, ther is good agreement. The only exception is a significant increase of the proportion of former smokers in the past years.", "contents": "[Sample survey of smoking habits in the German, French and Romansch speaking parts of Switzerland in 1975]. In a survey of a representative sample of 900 persons in Switzerland (excluding the Italian speaking parts comprising some 4-5 percent of the population), the smoking habits of the adult population were studied. Smokers were defined as persons smoking at least one cigarette or an equivalent weight of pipe tobacco or cigars per day. 51.8% of men and 29.0% of women over age 15 are smokers. Among male smokers of cigarettes only (42% of all men), over three quarters (77.6%) smoke ten cigarettes or more per day, i.e., a quantity found harmful to health; over one half (55.8%) smoke twenty or more cigarettes per day. Half of all female smokers smoke ten or more cigarettes, more than one in every four female smokers (28.8%) smoke twenty or more cigarettes per day. It is calculated that almost two thirds of all cigarettes sold in Switzerland are smoked by smokers of twenty or more cigarettes per day, and that almost three quarters are smoked by smokers of ten or more cigarettes. Among men, smoking habits are independent of social status, whereas among women, those with higher family incomes show more frequent smoking, but also more widespread cessation of smoking than with lower incomes. Thirty percent of adults having been smokers have given up the habit, so that among men and women above age 15, theree are now some 20% and 14% respectively, of ex-smokers. This corresponds to at least 700,000 former smokers in all of Switzerland. The trend to give up starts already in the group aged 15-24 (over 20% of men and women having ever smoked have given up) and becomes more marked with increasing age. A large part of male and female smokers would like to free themselves of the habit. 30% of male and 34% of female smokers indicate that they had tried seriously to stop smoking during the twelve months preceding the interview. Trying to give up was not significantly associated with intensity of smoking or social status. According to this survey, a majority of the population (57.6%) is in favor of reserving certain parts of restaurants to non-smokers, following the example of non-smokers-compartments in railway trains. In a situation where a non-smoker asks a co-worker in a closed room not to smoke, 78.3% take sides with the non-smoker, either by conceding to him a right to smoke-free air, or by demanding from the smoker to show consideration for the non-smoker. Only 10.9% demand from the non-smoker understanding for the smoker, or concede to the smoker a right to smoke at work. Women show more understanding for cause of the non-smoker than men. This difference is more marked among smokers and former smokers than among non-smokers. Inasmuch as the results of this study are comparable to those of earlier surveys conducted in Switzerland, ther is good agreement. The only exception is a significant increase of the proportion of former smokers in the past years.", "PMID": 969915}
{"id": "pubmed23n0032_7671", "title": "SH Reactivity of cigarette smoke and its correlation with carcinogenic effects on hamster lung cultures.", "content": "After hamster lung cultures were exposed repeatedly to puffs of fresh smoke from 7 types of cigarettes containing variable amounts of particulate and gas vapour phase components, atypical growth and/or malignant cell transformation were observed within a period of 3-6 months. A positive correlation was demonstrable between high SH reactivity and high NO content of the gas vapour phase and malignant transformation. There was no positive correlation for the other analyzed components of the smoke, including tar content.", "contents": "SH Reactivity of cigarette smoke and its correlation with carcinogenic effects on hamster lung cultures. After hamster lung cultures were exposed repeatedly to puffs of fresh smoke from 7 types of cigarettes containing variable amounts of particulate and gas vapour phase components, atypical growth and/or malignant cell transformation were observed within a period of 3-6 months. A positive correlation was demonstrable between high SH reactivity and high NO content of the gas vapour phase and malignant transformation. There was no positive correlation for the other analyzed components of the smoke, including tar content.", "PMID": 969919}
{"id": "pubmed23n0032_7672", "title": "[The quantitative evaluation of alcohol use in German and Western Switzerland].", "content": "Within the scope of a comprehensive study of social mechanisms of the drinking habits, a household survey was conducted among the 15 to 74-year-old population (sample size: 902). Overall per-capita-consumption figures have been, so far, the only available information on drinking in Switzerland. The data collected on various dimensions, i.e. quantity, frequency and variability of alcohol intake show a wide variety of drinking patterns among the 89% of the population using alcohol. Frequency of beer, wine and fermented cider consumption falls mainly into the three categories: daily use, up to twice weekly, rarely (once a month or less). For hard liquor we found: up to twice weekly, rarely. The Swiss have no definite preferences as to wine, beer or other alcoholic beverages. Yet, in the French speaking part wine is used significantly...", "contents": "[The quantitative evaluation of alcohol use in German and Western Switzerland]. Within the scope of a comprehensive study of social mechanisms of the drinking habits, a household survey was conducted among the 15 to 74-year-old population (sample size: 902). Overall per-capita-consumption figures have been, so far, the only available information on drinking in Switzerland. The data collected on various dimensions, i.e. quantity, frequency and variability of alcohol intake show a wide variety of drinking patterns among the 89% of the population using alcohol. Frequency of beer, wine and fermented cider consumption falls mainly into the three categories: daily use, up to twice weekly, rarely (once a month or less). For hard liquor we found: up to twice weekly, rarely. The Swiss have no definite preferences as to wine, beer or other alcoholic beverages. Yet, in the French speaking part wine is used significantly...", "PMID": 969921}
{"id": "pubmed23n0032_7673", "title": "[Signs of the zodiac and personality].", "content": "3074 young men resident in the canton of Zurich, representing 50% of the 19 year old male population, form the fully representative sample of our large scale investigation. We investigated whether personality traits measured by means of the differentiated \"Freiburger personality inventory\" (FPI) could in any way be correlated to the signs of the zodiac under which the young men were born. The statistical analysis did not reveal any correlation between signs of the zodiac and personality. The claim made by astrologers that people can be characterized according to their sign of the zodiac (sagitarius, taurus, cancer, scorpion) must be refuted. Of course the astrologically founded description of human personality does not base itself on the position of the sun only, however the latter does form a very essential part of the astrological evaluation of people. This, at any rate has been shown to be without any scientific basis. The fact that astrological evaluation of human personality is so popular nowadays can be explained by the fact that even modern people are inclined towards magical thinking.", "contents": "[Signs of the zodiac and personality]. 3074 young men resident in the canton of Zurich, representing 50% of the 19 year old male population, form the fully representative sample of our large scale investigation. We investigated whether personality traits measured by means of the differentiated \"Freiburger personality inventory\" (FPI) could in any way be correlated to the signs of the zodiac under which the young men were born. The statistical analysis did not reveal any correlation between signs of the zodiac and personality. The claim made by astrologers that people can be characterized according to their sign of the zodiac (sagitarius, taurus, cancer, scorpion) must be refuted. Of course the astrologically founded description of human personality does not base itself on the position of the sun only, however the latter does form a very essential part of the astrological evaluation of people. This, at any rate has been shown to be without any scientific basis. The fact that astrological evaluation of human personality is so popular nowadays can be explained by the fact that even modern people are inclined towards magical thinking.", "PMID": 969917}
{"id": "pubmed23n0032_7674", "title": "[Air pollution and irritations due to cigarette smoke].", "content": "In a nearly airtight climatic chamber of 30 m3 we studied the air pollution due to cigarette smoke as well as resulting irritations and annoyance. When 9 cig/30 m3 have been smoked, acroleine reaches the threshold limit value for industries; when 5 cig/30 m3 have been smoked, the clean air standards for outdoor air are exceeded by acroleine, carbon monoxide, and formaldehyde. The eyes are the most sensitive organ to cigarette smoke, followed by the nose. Annoyance about air quality and the wish to open the door or to leave the room proved to be other susceptible criteria. With 10 cig/30 m3, 9% of the subjects show a \"strong\" or \"very strong\" eye irritation, while 78% \"wish to leave the room=. The most important of the measured irritants seems to be acroleine.", "contents": "[Air pollution and irritations due to cigarette smoke]. In a nearly airtight climatic chamber of 30 m3 we studied the air pollution due to cigarette smoke as well as resulting irritations and annoyance. When 9 cig/30 m3 have been smoked, acroleine reaches the threshold limit value for industries; when 5 cig/30 m3 have been smoked, the clean air standards for outdoor air are exceeded by acroleine, carbon monoxide, and formaldehyde. The eyes are the most sensitive organ to cigarette smoke, followed by the nose. Annoyance about air quality and the wish to open the door or to leave the room proved to be other susceptible criteria. With 10 cig/30 m3, 9% of the subjects show a \"strong\" or \"very strong\" eye irritation, while 78% \"wish to leave the room=. The most important of the measured irritants seems to be acroleine.", "PMID": 969923}
{"id": "pubmed23n0032_7675", "title": "[Effects of air pollution on health].", "content": "Air pollution is composed of a particulate phase and a gaseous phase. The particulate phase, consisting of dust and aerosols, is the vehicle of toxic pollutants, transporting them into the respiratory tract. The irritants (sulphur dioxide, nitrogen oxides, aldehydes, and hydrocarbons) are the most important pollutants as regards health; the mucous membranes of the eyes and the respiratory tract are mostly affected. Other substances penetrate into the organism where they can develop actions harmful to various organs (carbon monoxide), generate cancer (polyaromatic hydrocarbons) or attack enzymatic systems (lead). Epidemiologic studies have revealed that populations exposed to high degrees of air pollution show increased mortality, which is mainly due to illnesses of the respiratory tract. Atmospheric pollution can also cause sensations of unpleasantness due to irritations of the eyes and the respiratory tract, bad odours, and the impairment of visibility and sunlight.", "contents": "[Effects of air pollution on health]. Air pollution is composed of a particulate phase and a gaseous phase. The particulate phase, consisting of dust and aerosols, is the vehicle of toxic pollutants, transporting them into the respiratory tract. The irritants (sulphur dioxide, nitrogen oxides, aldehydes, and hydrocarbons) are the most important pollutants as regards health; the mucous membranes of the eyes and the respiratory tract are mostly affected. Other substances penetrate into the organism where they can develop actions harmful to various organs (carbon monoxide), generate cancer (polyaromatic hydrocarbons) or attack enzymatic systems (lead). Epidemiologic studies have revealed that populations exposed to high degrees of air pollution show increased mortality, which is mainly due to illnesses of the respiratory tract. Atmospheric pollution can also cause sensations of unpleasantness due to irritations of the eyes and the respiratory tract, bad odours, and the impairment of visibility and sunlight.", "PMID": 969925}
{"id": "pubmed23n0032_7676", "title": "[Air pollution in an industrial environment and occupational diseases].", "content": "Most of the occupational diseases are related to air pollution at the work place. The distribution of gases and vapours and their human absorption are briefly described. The deposition of particles is a function of their physical properties, of the pattern of the respiratory airways and of pulmonary ventilation parameters. Several defence mechanisms also play a role. Illustrative examples are given of occupational diseases caused by solvents, carbon monoxide, metals, dusts (silicosis, asbestosis, pneumoconiosis due to dust of hard metals), allergens and carcinogens. The importance of prevention is emphasized.", "contents": "[Air pollution in an industrial environment and occupational diseases]. Most of the occupational diseases are related to air pollution at the work place. The distribution of gases and vapours and their human absorption are briefly described. The deposition of particles is a function of their physical properties, of the pattern of the respiratory airways and of pulmonary ventilation parameters. Several defence mechanisms also play a role. Illustrative examples are given of occupational diseases caused by solvents, carbon monoxide, metals, dusts (silicosis, asbestosis, pneumoconiosis due to dust of hard metals), allergens and carcinogens. The importance of prevention is emphasized.", "PMID": 969926}
{"id": "pubmed23n0032_7677", "title": "[Atmospheric allergens].", "content": "Aeromicrobiology techniques allow to determine nature and quantitative distribution of atmospheric particles which are capable of inducing an allergic reaction. Airborne pollens and seasonal mold spores are the main known aeroallergens. Their allergenicity has been clinically and biologically demonstrated and is indeed responsible for pathological manifestations in man especially within the respiratory tract.", "contents": "[Atmospheric allergens]. Aeromicrobiology techniques allow to determine nature and quantitative distribution of atmospheric particles which are capable of inducing an allergic reaction. Airborne pollens and seasonal mold spores are the main known aeroallergens. Their allergenicity has been clinically and biologically demonstrated and is indeed responsible for pathological manifestations in man especially within the respiratory tract.", "PMID": 969922}
{"id": "pubmed23n0032_7678", "title": "[Climate and air pollution].", "content": "Climate and air pollution are closely related. On the one hand, the climate is more and more influenced, locally as well as globally, by the tremendous concentration of pollutants in our atmosphere. Certain elements of the climate are, on the other hand, directly responsible for the process of dilution and dispersion of pollution emission. Of particular importance is the phenomen of ventilation and inversion. The significance of knowing the regional climatic peculiarities is presented by ways of a smallspace-example of the ventilation existing at the valley of river Aare and of the frequency of inversion existing at the Aare-trench situated north of Berne. Such knowledge should increasingly be used to influence regional planning, to assist in the determination of the position of any important source of pollution and should help to determine the selection of suitable living and recreation areas. Unfavorable meteorological-climatological conditions could aggravate a situation whereby harmless concentrations of pollutants could lead locally to critical proportions. For the better understanding of the meteorological and climatological processes within the lowest 500 m-level of our atmosphere, an increased effort of research should be considered in this connection. Through the use of improved data processing in connection with more closely meshed measuring networks, and of automatic recording facilities, as well as through the increased use of physical models, the problem of immission in our heavily valleyed country could be investigated more thoroughly.", "contents": "[Climate and air pollution]. Climate and air pollution are closely related. On the one hand, the climate is more and more influenced, locally as well as globally, by the tremendous concentration of pollutants in our atmosphere. Certain elements of the climate are, on the other hand, directly responsible for the process of dilution and dispersion of pollution emission. Of particular importance is the phenomen of ventilation and inversion. The significance of knowing the regional climatic peculiarities is presented by ways of a smallspace-example of the ventilation existing at the valley of river Aare and of the frequency of inversion existing at the Aare-trench situated north of Berne. Such knowledge should increasingly be used to influence regional planning, to assist in the determination of the position of any important source of pollution and should help to determine the selection of suitable living and recreation areas. Unfavorable meteorological-climatological conditions could aggravate a situation whereby harmless concentrations of pollutants could lead locally to critical proportions. For the better understanding of the meteorological and climatological processes within the lowest 500 m-level of our atmosphere, an increased effort of research should be considered in this connection. Through the use of improved data processing in connection with more closely meshed measuring networks, and of automatic recording facilities, as well as through the increased use of physical models, the problem of immission in our heavily valleyed country could be investigated more thoroughly.", "PMID": 969927}
{"id": "pubmed23n0032_7679", "title": "[Epidemiology of chronic bronchitis].", "content": "In this short paper, the definition of chronic bronchitis, as it is now widely accepted by epidemiologists, is given and a comparison is made with the disease as usually referred to by general practitioners. Risk factors which are related to the development of the disease are then briefly discussed and the importance of internal parameters is underlined. At last, the methodological difficulties which arise in the assessment of the relationship between air pollution and respiratory symptoms are emphasized.", "contents": "[Epidemiology of chronic bronchitis]. In this short paper, the definition of chronic bronchitis, as it is now widely accepted by epidemiologists, is given and a comparison is made with the disease as usually referred to by general practitioners. Risk factors which are related to the development of the disease are then briefly discussed and the importance of internal parameters is underlined. At last, the methodological difficulties which arise in the assessment of the relationship between air pollution and respiratory symptoms are emphasized.", "PMID": 969928}
{"id": "pubmed23n0032_7680", "title": "[Leiomyomas and fusiform neuromas as intramural tumors of the thoracic oesophagus (author's transl)].", "content": "On the 2nd Surgical Department of Vienna, only 2.61% of all resected oesophageal tumors are leiomyomas and fusiform neuromas Contrary to the opinion of the most other authors, fusiform neuromas are predominating in our cases. This fact could be explained by the exact histological evaluation which strictly follows the instructions published by Feyrter. The patients are young or middle-aged and suffer from dysphageal and pulmonal troubles. Indication for operation is not only founded on the clinical symptoms, but also on the possibility of a malign character of the tumour. Intramural tumours of the thoracical oesophagus should be ennucleated via a transpleural thoracotomy by preserving the mucoseal tube. Differential diagnosis and clinical experiences are resumed and discussed.", "contents": "[Leiomyomas and fusiform neuromas as intramural tumors of the thoracic oesophagus (author's transl)]. On the 2nd Surgical Department of Vienna, only 2.61% of all resected oesophageal tumors are leiomyomas and fusiform neuromas Contrary to the opinion of the most other authors, fusiform neuromas are predominating in our cases. This fact could be explained by the exact histological evaluation which strictly follows the instructions published by Feyrter. The patients are young or middle-aged and suffer from dysphageal and pulmonal troubles. Indication for operation is not only founded on the clinical symptoms, but also on the possibility of a malign character of the tumour. Intramural tumours of the thoracical oesophagus should be ennucleated via a transpleural thoracotomy by preserving the mucoseal tube. Differential diagnosis and clinical experiences are resumed and discussed.", "PMID": 969971}
{"id": "pubmed23n0032_7681", "title": "[Method and technique of experimental autotransplantation of a lung and a lung lobe].", "content": "The authors have accumulated an experience of 350 canine autotransplantations of a lung and its lobe. The peculiarities of the operative technique are described, and special emphasis is laid on its technical details that largely determine the success of a lung autotransplantation. The described method and technique permit the performance of orthotopic autotransplantation of a whole lung and of its lobe, and of heterotopic autotransplantation of the lower lobe of the right lung into the left pleural cavity in place of a removed lower lobe or whole lung. The first stage of autotransplantation of a lung or its lobe consists in applying an atrio-venous or venous anastomosis and that of the pulmonary artery with continuous U-shaped eversion sutures, with a re-establishment of the circulation, thus reducing the period of lung ischaemia. Upon restoring the blood flow in the lung, a bronchial anastomosis is applied with interrupted or continuous sutures, and the ventilation is restored. The vascular and bronchial anastomoses are applied with synthetic suture material (Orsylon) on atraumatic needles. The extensive experience of the authors in long-term follow-up of the animals after transplantation (up to 5 years) demonstrates that the described technique and method of lung autotransplantation ensures good competence and patency of the vascular and bronchial anastomoses, without any stenoses observed in the late postoperative period. As shown by histological examinations, the inflammatory reaction around the suture material is minimal.", "contents": "[Method and technique of experimental autotransplantation of a lung and a lung lobe]. The authors have accumulated an experience of 350 canine autotransplantations of a lung and its lobe. The peculiarities of the operative technique are described, and special emphasis is laid on its technical details that largely determine the success of a lung autotransplantation. The described method and technique permit the performance of orthotopic autotransplantation of a whole lung and of its lobe, and of heterotopic autotransplantation of the lower lobe of the right lung into the left pleural cavity in place of a removed lower lobe or whole lung. The first stage of autotransplantation of a lung or its lobe consists in applying an atrio-venous or venous anastomosis and that of the pulmonary artery with continuous U-shaped eversion sutures, with a re-establishment of the circulation, thus reducing the period of lung ischaemia. Upon restoring the blood flow in the lung, a bronchial anastomosis is applied with interrupted or continuous sutures, and the ventilation is restored. The vascular and bronchial anastomoses are applied with synthetic suture material (Orsylon) on atraumatic needles. The extensive experience of the authors in long-term follow-up of the animals after transplantation (up to 5 years) demonstrates that the described technique and method of lung autotransplantation ensures good competence and patency of the vascular and bronchial anastomoses, without any stenoses observed in the late postoperative period. As shown by histological examinations, the inflammatory reaction around the suture material is minimal.", "PMID": 969972}
{"id": "pubmed23n0032_7682", "title": "[Valgus deformity of the femoral neck after nailing of infantile femoral shaft fractures (author's transl)].", "content": "In 6 patients out of 29 who had suffered fractures of the femoral shaft at childs age and were treated mainly by open nailing, considerable growth disturbances of the major trochanter and valgus deformities of the neck of the femur had been observed in check-ups. The origin and prevention of these complications in nailing and nailremoval are discussed.", "contents": "[Valgus deformity of the femoral neck after nailing of infantile femoral shaft fractures (author's transl)]. In 6 patients out of 29 who had suffered fractures of the femoral shaft at childs age and were treated mainly by open nailing, considerable growth disturbances of the major trochanter and valgus deformities of the neck of the femur had been observed in check-ups. The origin and prevention of these complications in nailing and nailremoval are discussed.", "PMID": 969976}
{"id": "pubmed23n0032_7683", "title": "[Necrotizing enterocolitis in babies (author's transl)].", "content": "Newborns and babies weakened in their resistance will mainly be affected with necrotizing enterocolitis. A conservative therapy is principally applied. It is only by increasing signs of peritonitis or caused by perforation of the intestine that laparotomy is required. A report is presented on 11 babies with surgical therapy and the kind of operative procedure applied. 6 of these babies were cured.", "contents": "[Necrotizing enterocolitis in babies (author's transl)]. Newborns and babies weakened in their resistance will mainly be affected with necrotizing enterocolitis. A conservative therapy is principally applied. It is only by increasing signs of peritonitis or caused by perforation of the intestine that laparotomy is required. A report is presented on 11 babies with surgical therapy and the kind of operative procedure applied. 6 of these babies were cured.", "PMID": 969977}
{"id": "pubmed23n0032_7684", "title": "[The reconstruction of inflammatory biliary duct stenoses by using a ring-drainage (author's transl)].", "content": "In treating the inflammatory closures and stenoses of the biliary ducts surgically, the bilio-biliary and bilio-digestive anastomoses were splinted by inserting an annular drainage. In this way it was possible to ensure the bileflow into the intestine and to prevent recurrent stenoses. The ring-drainage is suggested to be kept for 2 years at least. In our experience this method has been validated in 13 patients. Therefore it is recommended for use in reconstructive operations with inflammatory obstructions of the biliary tract.", "contents": "[The reconstruction of inflammatory biliary duct stenoses by using a ring-drainage (author's transl)]. In treating the inflammatory closures and stenoses of the biliary ducts surgically, the bilio-biliary and bilio-digestive anastomoses were splinted by inserting an annular drainage. In this way it was possible to ensure the bileflow into the intestine and to prevent recurrent stenoses. The ring-drainage is suggested to be kept for 2 years at least. In our experience this method has been validated in 13 patients. Therefore it is recommended for use in reconstructive operations with inflammatory obstructions of the biliary tract.", "PMID": 969978}
{"id": "pubmed23n0032_7685", "title": "[Treatment of functional sterility using Clostylbegyt].", "content": "Report of 39 patients suffering from functional sterility, having been treated with Clostylbegyt. Under analysis were the age of the patients, complications during therapy, time of ovulation and the effect of the drug on several ovarian caused difficulties of conception. Controlling examinations as well as the pregnancies obtained and their progress were discussed.", "contents": "[Treatment of functional sterility using Clostylbegyt]. Report of 39 patients suffering from functional sterility, having been treated with Clostylbegyt. Under analysis were the age of the patients, complications during therapy, time of ovulation and the effect of the drug on several ovarian caused difficulties of conception. Controlling examinations as well as the pregnancies obtained and their progress were discussed.", "PMID": 969998}
{"id": "pubmed23n0032_7686", "title": "[Current organizational and clinical aspects of sterility and infertility].", "content": "It is reported about an organizational plan for more intensity in the field of medical care and research of sterility and infertility. Diagnosis and therapy of sterility should be carried out within a high specialised centre. General practitioner and gynecologist in general and clinical practice of the territory have to accomplish important problems in prophylaxis and metaphylaxis of sterility and infertility.", "contents": "[Current organizational and clinical aspects of sterility and infertility]. It is reported about an organizational plan for more intensity in the field of medical care and research of sterility and infertility. Diagnosis and therapy of sterility should be carried out within a high specialised centre. General practitioner and gynecologist in general and clinical practice of the territory have to accomplish important problems in prophylaxis and metaphylaxis of sterility and infertility.", "PMID": 969997}
{"id": "pubmed23n0032_7687", "title": "[Behavior of heparinocytes and of fibrinolytic degradation products after discontinuation of hormonal contraceptives].", "content": "After cessation of hormonal contraception a quick adaptation of the heparinocyte values to the normal menstrual phase course appears, but the values occur at a higher level. Fibrinolytic split products in the blood are really elevated nearly without exception in the first 4 to 6 weeks after ceasing of hormonal contraception. Foremost after the first spontaneous menstrual bleeding they are at a normal level.", "contents": "[Behavior of heparinocytes and of fibrinolytic degradation products after discontinuation of hormonal contraceptives]. After cessation of hormonal contraception a quick adaptation of the heparinocyte values to the normal menstrual phase course appears, but the values occur at a higher level. Fibrinolytic split products in the blood are really elevated nearly without exception in the first 4 to 6 weeks after ceasing of hormonal contraception. Foremost after the first spontaneous menstrual bleeding they are at a normal level.", "PMID": 969999}
{"id": "pubmed23n0032_7688", "title": "[Changes of the endometrium during oral hormonal contraception with ethinyl estradiol sulfonate and norethisterone acetate or d-noregestrel].", "content": "48 fertile women took in three weeks 1 mg ethinylestradiolsulfonate weekly and in the 4th week 10 mg norethisterone acetate, respectively d-norgestrel in different dosages (0,5 mgs, 1,0 mgs, 1,5 mgs or 3,0 mgs). On this 48 women 69 abrasiones were made between the first and 37th treatment cycles in order to estimate the endometrium. Ethinylestradiolsulfonate caused for the most part a proliferation of endometrium, which reached to a glandulare cystic hyperplastic endometrium. We did not observe a reduction of the endometrial respond to this depot-estrogen in dependence on the time of taking. Norethisterone acetate and d-norgestrel effected on a different proliferated endometrium a secretory transfer mation always.", "contents": "[Changes of the endometrium during oral hormonal contraception with ethinyl estradiol sulfonate and norethisterone acetate or d-noregestrel]. 48 fertile women took in three weeks 1 mg ethinylestradiolsulfonate weekly and in the 4th week 10 mg norethisterone acetate, respectively d-norgestrel in different dosages (0,5 mgs, 1,0 mgs, 1,5 mgs or 3,0 mgs). On this 48 women 69 abrasiones were made between the first and 37th treatment cycles in order to estimate the endometrium. Ethinylestradiolsulfonate caused for the most part a proliferation of endometrium, which reached to a glandulare cystic hyperplastic endometrium. We did not observe a reduction of the endometrial respond to this depot-estrogen in dependence on the time of taking. Norethisterone acetate and d-norgestrel effected on a different proliferated endometrium a secretory transfer mation always.", "PMID": 970000}
{"id": "pubmed23n0032_7689", "title": "[Specific changes of the uterine cervix due to contraceptives].", "content": "A group of 344 cervices, produced by conisation within a period of 5 1/2 years, were examined for specific effects of contraceptives. In half of all women, who used oral contraceptives, specific lesions such as \"siebartige polypoide Hyperplasie\" (cribriform polypoid hyperplasia) were found. The findings and their etiology and importance are discussed.", "contents": "[Specific changes of the uterine cervix due to contraceptives]. A group of 344 cervices, produced by conisation within a period of 5 1/2 years, were examined for specific effects of contraceptives. In half of all women, who used oral contraceptives, specific lesions such as \"siebartige polypoide Hyperplasie\" (cribriform polypoid hyperplasia) were found. The findings and their etiology and importance are discussed.", "PMID": 970001}
{"id": "pubmed23n0032_7690", "title": "[Methods of intrauterine pressure measurements in non-pregnant women].", "content": "It is described a method of measurement of intrauterine pressure with perfusion of catheter by nonpregnant women. It is possible to prevent arteficial disturbances on account of obstruction of catheter.", "contents": "[Methods of intrauterine pressure measurements in non-pregnant women]. It is described a method of measurement of intrauterine pressure with perfusion of catheter by nonpregnant women. It is possible to prevent arteficial disturbances on account of obstruction of catheter.", "PMID": 970003}
{"id": "pubmed23n0032_7691", "title": "[Vaginal rupture with prolapse of the small intestine].", "content": "We report on a case of recidival rupture of an enterocele with vaginal rupture and incarceration of small bowel. This event was probably caused by an extreme insufficiency of the patient's connective tissue. In literature four similar cases have been reported.", "contents": "[Vaginal rupture with prolapse of the small intestine]. We report on a case of recidival rupture of an enterocele with vaginal rupture and incarceration of small bowel. This event was probably caused by an extreme insufficiency of the patient's connective tissue. In literature four similar cases have been reported.", "PMID": 970004}
{"id": "pubmed23n0032_7692", "title": "[Relations hip between placental weight and age, family characteristics, parity and activity of the mother as well as the weight or maturity and sex of the newborn infant]?1?H.", "content": "An investigation of the correlation of the placental weights of 500 unselected live-born babies delivered after a gestation period of 33 weeks at the minimum and 42 weeks at the maximum, respectively, with age, family status parity, and mother's occupation, as well as with the weight, maturity, and sex of the neonates yielded the following results: 1. Between primiparae, secundiparae, and multiparae, working and housewife mothers, workers, farmerettes, women doing other jobs, and academically trained women, eutrophic mature and hypertrophic mature neonates, and newly born male and female babies there was observed a roughly similar frequency of the generally accepted \"normal weight of placenta\" of 400 g to 600 g and no significance of minor differences in frequency, respectively. 2. The high incidence of mothers aged 20 and less in the \"normal placental weight group\" of 400 g to 600 g compared to 21- to 30-year-old and 31- to 35-year-old mothers was not found to be significant. 3. The high incidence of unmarried mothers in the \"normal placental weight group\" of 400 g to 600 g compared to married mothers is not significant, the higher incidence of premature labor in unmarried women being due possibly, not to the weight of placenta, but rather primiparity, age under 20, and social class. 4. The higher frequency in the placental weight group up to and including 400 g of married compared to unmarried mothers, primiparae compared to secundiparae, primiparae and secundiparae compared to multiparae, working mothers compared to housewife mothers, and workers and farmerettes compared to both women doing other types of jobs and academically trained women was not found to be significant.", "contents": "[Relations hip between placental weight and age, family characteristics, parity and activity of the mother as well as the weight or maturity and sex of the newborn infant]?1?H. An investigation of the correlation of the placental weights of 500 unselected live-born babies delivered after a gestation period of 33 weeks at the minimum and 42 weeks at the maximum, respectively, with age, family status parity, and mother's occupation, as well as with the weight, maturity, and sex of the neonates yielded the following results: 1. Between primiparae, secundiparae, and multiparae, working and housewife mothers, workers, farmerettes, women doing other jobs, and academically trained women, eutrophic mature and hypertrophic mature neonates, and newly born male and female babies there was observed a roughly similar frequency of the generally accepted \"normal weight of placenta\" of 400 g to 600 g and no significance of minor differences in frequency, respectively. 2. The high incidence of mothers aged 20 and less in the \"normal placental weight group\" of 400 g to 600 g compared to 21- to 30-year-old and 31- to 35-year-old mothers was not found to be significant. 3. The high incidence of unmarried mothers in the \"normal placental weight group\" of 400 g to 600 g compared to married mothers is not significant, the higher incidence of premature labor in unmarried women being due possibly, not to the weight of placenta, but rather primiparity, age under 20, and social class. 4. The higher frequency in the placental weight group up to and including 400 g of married compared to unmarried mothers, primiparae compared to secundiparae, primiparae and secundiparae compared to multiparae, working mothers compared to housewife mothers, and workers and farmerettes compared to both women doing other types of jobs and academically trained women was not found to be significant.", "PMID": 970005}
{"id": "pubmed23n0032_7693", "title": "[Low weight and endangering of multiple birth infants].", "content": "Multiple pregnancy represents a high risk to the survival of fetuses since insufficient nutrition frequently results in hypotrophic development and low birth weight. The frequency of the occurrence of low birth weight in children from a multiple birth is 10 times higher than in all newborn children and the early noenatal mortality of children froma multiple birth is 8 times higher than that of all newborn infants. The disproportion between the placenta and the fetuses which we usually find in multiple pregnancies may result in the occurrence of cerebral damage. In the period 1965 to 1967 perinatal death associated with cerebral damage by birth injury (recorded on the death certificate) accounted for 5,7% of all children of low birth weight and for 0,2% of children weighing at birth over 2500 g.", "contents": "[Low weight and endangering of multiple birth infants]. Multiple pregnancy represents a high risk to the survival of fetuses since insufficient nutrition frequently results in hypotrophic development and low birth weight. The frequency of the occurrence of low birth weight in children from a multiple birth is 10 times higher than in all newborn children and the early noenatal mortality of children froma multiple birth is 8 times higher than that of all newborn infants. The disproportion between the placenta and the fetuses which we usually find in multiple pregnancies may result in the occurrence of cerebral damage. In the period 1965 to 1967 perinatal death associated with cerebral damage by birth injury (recorded on the death certificate) accounted for 5,7% of all children of low birth weight and for 0,2% of children weighing at birth over 2500 g.", "PMID": 970006}
{"id": "pubmed23n0032_7694", "title": "[Histological diagnosis in aspiration curettage].", "content": "The techniques and diagnostic accuracies for histologic sampling of endometrial tissue after suction curettage performed on 161 patients are discussed. In 90% no tissue was retrieved by sharp curettage after suction curettage. Tissues with higher consistence like polyps were obtained only for 50%. However, it will be expected, that Crumbling tissues of cancer will be obtained.", "contents": "[Histological diagnosis in aspiration curettage]. The techniques and diagnostic accuracies for histologic sampling of endometrial tissue after suction curettage performed on 161 patients are discussed. In 90% no tissue was retrieved by sharp curettage after suction curettage. Tissues with higher consistence like polyps were obtained only for 50%. However, it will be expected, that Crumbling tissues of cancer will be obtained.", "PMID": 970007}
{"id": "pubmed23n0032_7695", "title": "[Effect of diuresis on the results of quantitative estrogen- and HCG determinations].", "content": "Investigations on the influence of the diuresis effect upon the results of quantitative oestrogen and HCG determinations revealed that the oestrogen values increase with the 24-hour amount of urine. Oliguria and polyuria affect oestrogen excretion to a considerable extent; they have to be taken into account in prenatal diagnosis of pregnancy. Comparative HCG checks show that HCG concentration also varies considerable depending on fluid excretion. Diagnostic errors can be diminished or avoided when the quantitative HCG assessment is made on the 24-hour urine and then referred to the whole amount of urine.", "contents": "[Effect of diuresis on the results of quantitative estrogen- and HCG determinations]. Investigations on the influence of the diuresis effect upon the results of quantitative oestrogen and HCG determinations revealed that the oestrogen values increase with the 24-hour amount of urine. Oliguria and polyuria affect oestrogen excretion to a considerable extent; they have to be taken into account in prenatal diagnosis of pregnancy. Comparative HCG checks show that HCG concentration also varies considerable depending on fluid excretion. Diagnostic errors can be diminished or avoided when the quantitative HCG assessment is made on the 24-hour urine and then referred to the whole amount of urine.", "PMID": 970008}
{"id": "pubmed23n0032_7696", "title": "[Determination of L-cystine aminopeptidase (serum oxytocinase, EC 3.4.1.2) during normal pregnancy].", "content": "It is given a method to the determination of cystine aminopeptidase activity in the serum in pregnancies. A time of few minutes is necessary for one determination. The determination is performed with a spectralphotometer or with the Reaction Rate Analyzer (LKB 8600) in using the chromogen substance 1-cystine-bis p-nitroanilide. This substance is solved in dimethylformamide. The normaly range of the 8th to the 41 th week of pregnancy is determinated in a number of 453. In twins the values were higher. The cystine aminopeptidase is nearly specifically in pregnancies and is produced in the plazenta. It is a statement of cellulary activity in the plazenta.", "contents": "[Determination of L-cystine aminopeptidase (serum oxytocinase, EC 3.4.1.2) during normal pregnancy]. It is given a method to the determination of cystine aminopeptidase activity in the serum in pregnancies. A time of few minutes is necessary for one determination. The determination is performed with a spectralphotometer or with the Reaction Rate Analyzer (LKB 8600) in using the chromogen substance 1-cystine-bis p-nitroanilide. This substance is solved in dimethylformamide. The normaly range of the 8th to the 41 th week of pregnancy is determinated in a number of 453. In twins the values were higher. The cystine aminopeptidase is nearly specifically in pregnancies and is produced in the plazenta. It is a statement of cellulary activity in the plazenta.", "PMID": 970009}
{"id": "pubmed23n0032_7697", "title": "[Hierdence of X-chromatin post partum].", "content": "Determinations of the X-chromatin frequency in buccal smears were performed in 33 pregnant women at the day before delivery and daily for seven days thereafter. In 23 women with a spontaneous delivery there was a statistically significant drop in the X-chromatin frequency after delivery being lowest on the second day. On the fifth day the X-chromatin frequency had reached the predelivery level. In 10 women with operative delivery by cesarean section and hormonal suppression of lactation no such changes in the X-chromatin-frequency have been noted.", "contents": "[Hierdence of X-chromatin post partum]. Determinations of the X-chromatin frequency in buccal smears were performed in 33 pregnant women at the day before delivery and daily for seven days thereafter. In 23 women with a spontaneous delivery there was a statistically significant drop in the X-chromatin frequency after delivery being lowest on the second day. On the fifth day the X-chromatin frequency had reached the predelivery level. In 10 women with operative delivery by cesarean section and hormonal suppression of lactation no such changes in the X-chromatin-frequency have been noted.", "PMID": 970010}
{"id": "pubmed23n0032_7698", "title": "[Automatic tocography using the Cardiff infusion system under cardiotocographic control with the fetal monitor BMT 504].", "content": "Clinical results of the induction of labour by means of the Cardiff-equipment. The fetal monitor BMT-504 (VEB Kombinat Mebger\u00e4tewerk Zw\u00f6nitz) has been used as cardiotocographic control unit. Labour has been induced with oxytocin (n = 38) or methyloxytocin (n = 30). 50 deliveries, which where induced by means of intravenous oxytocin infusion enables to be comparate the results. Using the automatic infusion the average induction-delivery interval was 4 hours 14 minutes, the average dosage of tocergic drugs was 2 IW oxytocin or 20 mug methyloxytocin. Using oxytocin for labour induction we found an increase in obstetric operative frequency caused by fetal distress. There where an increase in basal tone of uterine activity in this patients. Therefore we recommend methyloxytocin for the labour induction of high risk pregnancies. The safety factors, which are incorporated in the Cardiff-equipment have many advantages in obstetrical routine work, to determine fetal distress at an earlier stage. The results presented show that with this automatic infusion system labour can induced with even greater efficiency and safety. The equipment is very useful in accordance to the continuous increasing number of planned deliveries.", "contents": "[Automatic tocography using the Cardiff infusion system under cardiotocographic control with the fetal monitor BMT 504]. Clinical results of the induction of labour by means of the Cardiff-equipment. The fetal monitor BMT-504 (VEB Kombinat Mebger\u00e4tewerk Zw\u00f6nitz) has been used as cardiotocographic control unit. Labour has been induced with oxytocin (n = 38) or methyloxytocin (n = 30). 50 deliveries, which where induced by means of intravenous oxytocin infusion enables to be comparate the results. Using the automatic infusion the average induction-delivery interval was 4 hours 14 minutes, the average dosage of tocergic drugs was 2 IW oxytocin or 20 mug methyloxytocin. Using oxytocin for labour induction we found an increase in obstetric operative frequency caused by fetal distress. There where an increase in basal tone of uterine activity in this patients. Therefore we recommend methyloxytocin for the labour induction of high risk pregnancies. The safety factors, which are incorporated in the Cardiff-equipment have many advantages in obstetrical routine work, to determine fetal distress at an earlier stage. The results presented show that with this automatic infusion system labour can induced with even greater efficiency and safety. The equipment is very useful in accordance to the continuous increasing number of planned deliveries.", "PMID": 970011}
{"id": "pubmed23n0032_7699", "title": "[Screening for diabetes within the scope of prenatal care].", "content": "9482 pregnant women searched for the Department of Prenatale Care of the town Karl-Marx-Stadt between 1968 and 1972. Obesity were found in 6, 2 p.c., glycosuria in 5, 1 p.c., a history of diabetes with glycosuria in 0,5 p.c. and obesity with gylcosuria in 0,5 p.c. The perinatale mortality rate was heightened only in the last group (3,7 p.c.) 51 prediabetic pregnant women were found in these five years by sugar screening. Eight women developed a White class A nad four women a White class B diabetes between 3 and 7 years later.", "contents": "[Screening for diabetes within the scope of prenatal care]. 9482 pregnant women searched for the Department of Prenatale Care of the town Karl-Marx-Stadt between 1968 and 1972. Obesity were found in 6, 2 p.c., glycosuria in 5, 1 p.c., a history of diabetes with glycosuria in 0,5 p.c. and obesity with gylcosuria in 0,5 p.c. The perinatale mortality rate was heightened only in the last group (3,7 p.c.) 51 prediabetic pregnant women were found in these five years by sugar screening. Eight women developed a White class A nad four women a White class B diabetes between 3 and 7 years later.", "PMID": 970012}
{"id": "pubmed23n0032_7700", "title": "[Outline for the control of the course of fetal growth].", "content": "In a synoptic schema are presented the percentiles curves of the biparietal diameter, the sum of the sagittal and transversal thoracic diameter and the fetal weight (Lubchenco modif.). To attain the percentiles curves of the ultrasonic parameters we applied 5400 mensurations of the biparietal diameter and 1300 mensurations of the thorax. In the diagram are published the percentiles P 10, P 25, P 50, P 75, P 90. It is possible to diagnose a divergence between the cephalic and thoracic growth (dystrophia, hypertrophia) and estimate the gestation weeks.", "contents": "[Outline for the control of the course of fetal growth]. In a synoptic schema are presented the percentiles curves of the biparietal diameter, the sum of the sagittal and transversal thoracic diameter and the fetal weight (Lubchenco modif.). To attain the percentiles curves of the ultrasonic parameters we applied 5400 mensurations of the biparietal diameter and 1300 mensurations of the thorax. In the diagram are published the percentiles P 10, P 25, P 50, P 75, P 90. It is possible to diagnose a divergence between the cephalic and thoracic growth (dystrophia, hypertrophia) and estimate the gestation weeks.", "PMID": 970013}
{"id": "pubmed23n0032_7701", "title": "[Transformation of secretory cylindrial cells into ciliar cells in the epithelium of the human uterine cervix (functional transformation of highly differentiated cells)].", "content": "By electron microscopical investigations of the cylindrical epithelium of the human cervix uteri we could show that in ciliar cells a regeneration of ciliars is possible. There is a discussion about the question whether the pinching off of ciliars is the beginning of a transformation process into a secretory cell. The functional change of secretory cells to ciliar cells becomes obvious by the existence of so-called granulo-filamentary bodies in the cytoplasm of ciliar cells. These bodies grow by a granulo-filamentary transformation of secretory granules. As these granulo-filamentary bodies do not take place in the extrusion process but stay in the cytoplasm they may thus be used as an indicator which shows the finished transformation process of a secretory cell into a ciliar cell.", "contents": "[Transformation of secretory cylindrial cells into ciliar cells in the epithelium of the human uterine cervix (functional transformation of highly differentiated cells)]. By electron microscopical investigations of the cylindrical epithelium of the human cervix uteri we could show that in ciliar cells a regeneration of ciliars is possible. There is a discussion about the question whether the pinching off of ciliars is the beginning of a transformation process into a secretory cell. The functional change of secretory cells to ciliar cells becomes obvious by the existence of so-called granulo-filamentary bodies in the cytoplasm of ciliar cells. These bodies grow by a granulo-filamentary transformation of secretory granules. As these granulo-filamentary bodies do not take place in the extrusion process but stay in the cytoplasm they may thus be used as an indicator which shows the finished transformation process of a secretory cell into a ciliar cell.", "PMID": 970014}
{"id": "pubmed23n0032_7702", "title": "[A Czechoslovak injection-contraceptive agent administered once a month].", "content": "During a 20-month period, 26 women aged 38 years on the average (27 to 45 years) received single intramuscular injections of a combination, containing 60 mg of Superlutin caproate plus 10 mg of oestradiol valerate in 1 ml of oily solution, at 4-week intervals as a contraceptive. Altogether 255 cycles were checked. In 6 cycles, minor accessory bleeding occurred, mostly in younger women. No undesired pregnancy took place. Under the name Lutofollin, the injections were prepared at the Research Institute for Pharmacy and Biochemistry in Prague. The preparation is especially suitable for women aged 30 to 45 years, as well as for those intolerant to oral hormonal contraceptives.", "contents": "[A Czechoslovak injection-contraceptive agent administered once a month]. During a 20-month period, 26 women aged 38 years on the average (27 to 45 years) received single intramuscular injections of a combination, containing 60 mg of Superlutin caproate plus 10 mg of oestradiol valerate in 1 ml of oily solution, at 4-week intervals as a contraceptive. Altogether 255 cycles were checked. In 6 cycles, minor accessory bleeding occurred, mostly in younger women. No undesired pregnancy took place. Under the name Lutofollin, the injections were prepared at the Research Institute for Pharmacy and Biochemistry in Prague. The preparation is especially suitable for women aged 30 to 45 years, as well as for those intolerant to oral hormonal contraceptives.", "PMID": 970015}
{"id": "pubmed23n0032_7703", "title": "[Studies on the increase in the incidence of endometrial and ovarian in cancer in Czechoslovakia during 1960-1973].", "content": "During the time period of the last 12 years the incidence of the endometrium carcinoma in the whole CSSR rised to 155,5% in the consideration of the incidence in the years 1961 to 1963. In the same time period the incidence of the ovarial carcinoma has grown up to 117,8% only, what seems to be still in the limits of average incidence shift of all female malignancies in the CSSR (117,1%). The rising incidence of endometrium carcinoma affects primarily the elderly women, but testiefies also that this malignancy is among them today much more frequent. Therefore the rising incidence cannot be explained only as a manifestation of elderly of the female population. In agreement with this reality the endometrium carcinoma is today diagnosed significantly more frequently also among the younger women (at least since 40 years of age). The totals of all endometrium carcinomas rised even more than incidence. The gynaecologists meet today 162% of such malignancies in comparison with the numbers in the years 1961-1963. It means that in the whole CSSR yearly 500 endometrium carcinomas more are diagnosed than before 12 years. The incidence of cervix carcinoma dropped during the same period significantly and the endometrium carcinoma became therefore nearly as frequent as cervical malignancy (1:1,2). Possible reasons of these frequency changes are discussed.", "contents": "[Studies on the increase in the incidence of endometrial and ovarian in cancer in Czechoslovakia during 1960-1973]. During the time period of the last 12 years the incidence of the endometrium carcinoma in the whole CSSR rised to 155,5% in the consideration of the incidence in the years 1961 to 1963. In the same time period the incidence of the ovarial carcinoma has grown up to 117,8% only, what seems to be still in the limits of average incidence shift of all female malignancies in the CSSR (117,1%). The rising incidence of endometrium carcinoma affects primarily the elderly women, but testiefies also that this malignancy is among them today much more frequent. Therefore the rising incidence cannot be explained only as a manifestation of elderly of the female population. In agreement with this reality the endometrium carcinoma is today diagnosed significantly more frequently also among the younger women (at least since 40 years of age). The totals of all endometrium carcinomas rised even more than incidence. The gynaecologists meet today 162% of such malignancies in comparison with the numbers in the years 1961-1963. It means that in the whole CSSR yearly 500 endometrium carcinomas more are diagnosed than before 12 years. The incidence of cervix carcinoma dropped during the same period significantly and the endometrium carcinoma became therefore nearly as frequent as cervical malignancy (1:1,2). Possible reasons of these frequency changes are discussed.", "PMID": 970016}
{"id": "pubmed23n0032_7704", "title": "[Organization of the early diagnosis of the carcinoma corporis uteri].", "content": "The problems in early diagnosis of endometrial cancers are not sufficiently solved. Only atypical bleeding with subsequent curettage will result in success. At this time progredient carcinomas are frequent and the chance of recovery is small. Therefore a screening program of early diagnosis by small biopsy is developed and clinically proved.", "contents": "[Organization of the early diagnosis of the carcinoma corporis uteri]. The problems in early diagnosis of endometrial cancers are not sufficiently solved. Only atypical bleeding with subsequent curettage will result in success. At this time progredient carcinomas are frequent and the chance of recovery is small. Therefore a screening program of early diagnosis by small biopsy is developed and clinically proved.", "PMID": 970017}
{"id": "pubmed23n0032_7705", "title": "[Hygienic and bacteriological comparative studies in 50 hospitals. II. The role of staff in the distribution of pathogenic organisms in surgical wards (author's transl)].", "content": "Before hygienic measures are recommended to a hospital it is absolutely necessary for the source of infection and the paths of infection - both possible and to be expected - to be clearly established. In order to be able to evaluate the paths of spread, one of the basic prerequisites would be to carry out examinations with a view to determining the number of persons who are carriers of pathogenic germs. To this effect, bacteriological samples of the right hand, the skin at the forehead/hairline, the coat in the area of the navel and the throat were taken from the nursing staff in surgical wards in 50 different hospitals. At the same time details of hand disinfection and hand drying were collected by questioning and local inspection. In at least one of the 4 swabs from 72 persons (= 38%) and in two or more swabs from 26 persons (= 14%). Staph. aureus was detected. The hair was the most contaminated area, followed by the coat and hands, while the throat was the least contaminated zone. To prevent pathogenic germs being spread by hand, alcohol-containing preparations were prevalently used (in 79% of the wards inspected). The hygienic hand disinfection was inadequate in 53% of the surgical wards. In four wards the hands were washed with soap exclusively. 35% of the surgical wards used common towels and disposable towels were used almost as frequently. In the remaining cases common and disposable towels were employed simultaneously. Storage of the used and unused disposable towels was in the main unsatisfactory. In view of the conditions met with in the various hospitals, proper hand disinfection must be insisted on, i.e. the hands must first be disinfected with a tested preparation and then washed, as may be required. If time plays a major role, alcohol-containing preparations should be used. Towels for common use must no longer be available in hospitals because they are important carriers of pathogenic germs.", "contents": "[Hygienic and bacteriological comparative studies in 50 hospitals. II. The role of staff in the distribution of pathogenic organisms in surgical wards (author's transl)]. Before hygienic measures are recommended to a hospital it is absolutely necessary for the source of infection and the paths of infection - both possible and to be expected - to be clearly established. In order to be able to evaluate the paths of spread, one of the basic prerequisites would be to carry out examinations with a view to determining the number of persons who are carriers of pathogenic germs. To this effect, bacteriological samples of the right hand, the skin at the forehead/hairline, the coat in the area of the navel and the throat were taken from the nursing staff in surgical wards in 50 different hospitals. At the same time details of hand disinfection and hand drying were collected by questioning and local inspection. In at least one of the 4 swabs from 72 persons (= 38%) and in two or more swabs from 26 persons (= 14%). Staph. aureus was detected. The hair was the most contaminated area, followed by the coat and hands, while the throat was the least contaminated zone. To prevent pathogenic germs being spread by hand, alcohol-containing preparations were prevalently used (in 79% of the wards inspected). The hygienic hand disinfection was inadequate in 53% of the surgical wards. In four wards the hands were washed with soap exclusively. 35% of the surgical wards used common towels and disposable towels were used almost as frequently. In the remaining cases common and disposable towels were employed simultaneously. Storage of the used and unused disposable towels was in the main unsatisfactory. In view of the conditions met with in the various hospitals, proper hand disinfection must be insisted on, i.e. the hands must first be disinfected with a tested preparation and then washed, as may be required. If time plays a major role, alcohol-containing preparations should be used. Towels for common use must no longer be available in hospitals because they are important carriers of pathogenic germs.", "PMID": 970019}
{"id": "pubmed23n0032_7706", "title": "[Hygienic and bacteriological comparative studies in 50 hospitals. III. The efficacy of sterilization (author's transl)].", "content": "As part of an investigation carried out in 50 hospitals we particularly checked the sterilisers available for proper functioning and correct operation. The work group examined 461 sterilisation programmes in 338 sterilisation units (176 autoclaves, 153 hot-air apparatus and 9 ethylene oxide units) during the daily routine operation. The group carried out biological and physical inspections and carefully recorded the faults and deficiencies detected during the sterilisation procedure. It was found that only in 22% of the hospitals was a proper functioning and correct operation of all the sterilising units in use ensured. Technical faults and errors in operating the equipment and especially in the loading in 23% of the programmes prevented sterility. Almost 50% of the hot-air sterilisers and about a quarter of the autoclaves fail to produce a consistent satisfactory effect. The incidence of errors which occurred with ethylene oxide sterilisation cannot be generalised, since only a limited number of units were checked. A breakdown by functional areas and wards indicates that serious errors occur not only in the centralised large units but also in little-used small autoclaves in peripheral areas. A comparison with the literature shows that our results do not differ to any appreciable extent from those obtained by other workers, over the last twenty years. Here systematic inspections by external control authorities could improve the situation within a few years. Regular inspections to be carried out by the hospitals themselves using modern test methods are necessary, and need internal record-keeping. In spite of this, we cannot at present do without external inspections. We pin our hopes mainly in regular and thorough expert training of the staff and on much closer cooperation between hospital and hygienist.", "contents": "[Hygienic and bacteriological comparative studies in 50 hospitals. III. The efficacy of sterilization (author's transl)]. As part of an investigation carried out in 50 hospitals we particularly checked the sterilisers available for proper functioning and correct operation. The work group examined 461 sterilisation programmes in 338 sterilisation units (176 autoclaves, 153 hot-air apparatus and 9 ethylene oxide units) during the daily routine operation. The group carried out biological and physical inspections and carefully recorded the faults and deficiencies detected during the sterilisation procedure. It was found that only in 22% of the hospitals was a proper functioning and correct operation of all the sterilising units in use ensured. Technical faults and errors in operating the equipment and especially in the loading in 23% of the programmes prevented sterility. Almost 50% of the hot-air sterilisers and about a quarter of the autoclaves fail to produce a consistent satisfactory effect. The incidence of errors which occurred with ethylene oxide sterilisation cannot be generalised, since only a limited number of units were checked. A breakdown by functional areas and wards indicates that serious errors occur not only in the centralised large units but also in little-used small autoclaves in peripheral areas. A comparison with the literature shows that our results do not differ to any appreciable extent from those obtained by other workers, over the last twenty years. Here systematic inspections by external control authorities could improve the situation within a few years. Regular inspections to be carried out by the hospitals themselves using modern test methods are necessary, and need internal record-keeping. In spite of this, we cannot at present do without external inspections. We pin our hopes mainly in regular and thorough expert training of the staff and on much closer cooperation between hospital and hygienist.", "PMID": 970020}
{"id": "pubmed23n0032_7707", "title": "[On the hazards of nosocomial infections in dental treatment areas (author's transl)].", "content": "Using a standardized method of obtaining bacterial counts of surfaces by superficial application and subsequent incubation of agar lamina, density and kind of bacteria present were examined in treatment areas of both individual dentists praxis and of dental poli-clinics. The results obtained showed, that 1. typical bacteria of hospital infections (Micrococcus aureus, Klebsiella) were present in only small numbers in both individual dental praxis or in clinical dental treatment areas. 2. the medium level of contamination was higher in the average individual dental praxis than in comparable clinical dental areas. 3. the increase in bacterial contamination in the course of the daily treatmentperiod was considerably more pronounced in the individual praxis as compared to clinics. 4. the following must be considered as especially liable to contaminated by bacteria: towels used by several persons, the head of the dental turbine, the support of the suction device, the patient head rest, the height control bar of the dental chair, the instrument tray, the operating lamp and working garments.", "contents": "[On the hazards of nosocomial infections in dental treatment areas (author's transl)]. Using a standardized method of obtaining bacterial counts of surfaces by superficial application and subsequent incubation of agar lamina, density and kind of bacteria present were examined in treatment areas of both individual dentists praxis and of dental poli-clinics. The results obtained showed, that 1. typical bacteria of hospital infections (Micrococcus aureus, Klebsiella) were present in only small numbers in both individual dental praxis or in clinical dental treatment areas. 2. the medium level of contamination was higher in the average individual dental praxis than in comparable clinical dental areas. 3. the increase in bacterial contamination in the course of the daily treatmentperiod was considerably more pronounced in the individual praxis as compared to clinics. 4. the following must be considered as especially liable to contaminated by bacteria: towels used by several persons, the head of the dental turbine, the support of the suction device, the patient head rest, the height control bar of the dental chair, the instrument tray, the operating lamp and working garments.", "PMID": 970021}
{"id": "pubmed23n0032_7708", "title": "[Test for the efficacy of disinfectants at surfaces in test models. I. (communication:) Dependence of experimental results on the method of demonstration of surviving germs (swab and rinsing) (author's transl)].", "content": "For the testing of disinfectants at surfaces, the germs having survived at the surface are demonstrated by means of swabs according to the guide-lines of the Deutsche Gesellschaft fur Hygiene und Mikrobiologie (DGHM): after the period of exposure to the disinfectant, the surfaces were rubbed off with a damp swab, and the frictional surface of the swab was plated out on nutrient agar. The effectiveness of this technique was compared with the effectiveness of a rinsing method in a test model. In the rinsing process, the objects to be tested for surviving germs were shaken together with a suspension and with glass beads. Then the content of germs in the suspension was quantitatively determined by means of dilution tests and pour plates. The findings were evaluated according to the guide-lines for evaluation of the DGHM (less than or equal to 10 surviving germs = adequate efficacy). For the findings obtained by rinsing, the average number of surviving germs was also determined. For maldehyde solutions were used as disinfectants for the test models (time of exposure: 4 hours; temperature 22 to 25 degrees C). These disinfecting experiments were performed on raw smoothed as well as on varnished beech-wood. The experimental results showed that the criterion \"less than or equal to X surviving germs\" in itself does not mean clear evidence of the efficacy of a disinfectant. The one and only decisive criterion is the frequency of the statement that a certain disinfectant or a corresponding dilution of this disinfectant has shown adequate efficacy. Therefore, one single test according to the guide-lines of the DGHM is insufficient. The frequency of the finding \"adequate efficacy\" is not only dependent on the concentration of the disinfectant but also on the technique used for the demonstration of surviving germs. The swab method (according to the guide-lines of the DGHM) occasionally resulted in the finding \"adequate efficacy\" already if 10(4) to 10(5) surviving germs were demonstrable by the rinsing method. The range of formaldehyde concentrations for which the finding of adequate efficacy were present with a frequency between 20% (minimum) and 80% (maximum) amounted to 0.2-0.5% (varnished surface) and 1.1-2.5% (raw surface), respectively for the swab method. The respective figures for the rinsing method were 0.8-1.3% and 4.8-6.5%, respectively. When using the swab method, there is a slower increase in the efficacy of the disinfectant with concentration as compared with the rinsing method. The rinsing method is, therefore, more representative of the efficacy of a disinfectant than the swab method. On account of the results of this study, it is recommended for model experiments to recover the surviving germs quantitatively by the rinsing method and to determine their number.", "contents": "[Test for the efficacy of disinfectants at surfaces in test models. I. (communication:) Dependence of experimental results on the method of demonstration of surviving germs (swab and rinsing) (author's transl)]. For the testing of disinfectants at surfaces, the germs having survived at the surface are demonstrated by means of swabs according to the guide-lines of the Deutsche Gesellschaft fur Hygiene und Mikrobiologie (DGHM): after the period of exposure to the disinfectant, the surfaces were rubbed off with a damp swab, and the frictional surface of the swab was plated out on nutrient agar. The effectiveness of this technique was compared with the effectiveness of a rinsing method in a test model. In the rinsing process, the objects to be tested for surviving germs were shaken together with a suspension and with glass beads. Then the content of germs in the suspension was quantitatively determined by means of dilution tests and pour plates. The findings were evaluated according to the guide-lines for evaluation of the DGHM (less than or equal to 10 surviving germs = adequate efficacy). For the findings obtained by rinsing, the average number of surviving germs was also determined. For maldehyde solutions were used as disinfectants for the test models (time of exposure: 4 hours; temperature 22 to 25 degrees C). These disinfecting experiments were performed on raw smoothed as well as on varnished beech-wood. The experimental results showed that the criterion \"less than or equal to X surviving germs\" in itself does not mean clear evidence of the efficacy of a disinfectant. The one and only decisive criterion is the frequency of the statement that a certain disinfectant or a corresponding dilution of this disinfectant has shown adequate efficacy. Therefore, one single test according to the guide-lines of the DGHM is insufficient. The frequency of the finding \"adequate efficacy\" is not only dependent on the concentration of the disinfectant but also on the technique used for the demonstration of surviving germs. The swab method (according to the guide-lines of the DGHM) occasionally resulted in the finding \"adequate efficacy\" already if 10(4) to 10(5) surviving germs were demonstrable by the rinsing method. The range of formaldehyde concentrations for which the finding of adequate efficacy were present with a frequency between 20% (minimum) and 80% (maximum) amounted to 0.2-0.5% (varnished surface) and 1.1-2.5% (raw surface), respectively for the swab method. The respective figures for the rinsing method were 0.8-1.3% and 4.8-6.5%, respectively. When using the swab method, there is a slower increase in the efficacy of the disinfectant with concentration as compared with the rinsing method. The rinsing method is, therefore, more representative of the efficacy of a disinfectant than the swab method. On account of the results of this study, it is recommended for model experiments to recover the surviving germs quantitatively by the rinsing method and to determine their number.", "PMID": 970022}
{"id": "pubmed23n0032_7709", "title": "[Studies on the reappearing of a bacterial flora in drinking water after ozonization (author's transl)].", "content": "The use of ozone as a drinking water disinfectant is increasing. As opposed to the commonly used chlorine the disinfecting activity of ozone cannot be sustained in the water during the distribution process, and as a result recontamination and bacterial growth are not adequately under control. Information about the safety margins of this disinfecting procedure was obtained in experiments simulating the working conditions of a water plant including supply system. After treatment with 1.5 mg O3/1 water, specimens with no detectable remanent ozone were found to remain sterile for at least one month. However, during the passage of a pipeline of 1200 m length recontamination with and considerable growth of microorganisms was observed. After inoculating water sterilized by ozone with a normal population of water bacteria, the bacterial growth was more pronounced than in similar experiments with heat-sterilized water of the same origin. This may be an indication that the breakdown products of organic water contaminants (e.g. humic acid) - resulting from the ozone treatment - are better nutrients for the water bacteria than the original organic substances themselves.", "contents": "[Studies on the reappearing of a bacterial flora in drinking water after ozonization (author's transl)]. The use of ozone as a drinking water disinfectant is increasing. As opposed to the commonly used chlorine the disinfecting activity of ozone cannot be sustained in the water during the distribution process, and as a result recontamination and bacterial growth are not adequately under control. Information about the safety margins of this disinfecting procedure was obtained in experiments simulating the working conditions of a water plant including supply system. After treatment with 1.5 mg O3/1 water, specimens with no detectable remanent ozone were found to remain sterile for at least one month. However, during the passage of a pipeline of 1200 m length recontamination with and considerable growth of microorganisms was observed. After inoculating water sterilized by ozone with a normal population of water bacteria, the bacterial growth was more pronounced than in similar experiments with heat-sterilized water of the same origin. This may be an indication that the breakdown products of organic water contaminants (e.g. humic acid) - resulting from the ozone treatment - are better nutrients for the water bacteria than the original organic substances themselves.", "PMID": 970023}
{"id": "pubmed23n0032_7710", "title": "[The use of an enzyme-kinetic-method for bacteriological examinations of heavy polluted surface waters (author's transl)].", "content": "The greatest fraction of nutrients dissolved in water are organic substances which are decomposed, transformed and mineralized by heterotrophic microorganisms. For this reason, investigations concerning energy transfer in an aquatic ecosystem, or practical examinations of the pollution load of surface waters, cannot be set up when based exclusively on more or less good counts of microorganisms of the production or destruction group. Above all, it is important for heterotrophic bacteria that productivity parameters for these numerical estimations are also provided. PARSON and STRICKLAND were successful in measuring the uptake of organic material in waters with uptake-kinetic methods; HOBBIE and WRIGHT developed a method for measuring the uptake of marked test-substrates in certain concentration ranges. As a result, for these substrates it was possible to measure the turnover in natural populations. Consequently, after frequent improvement of the technique in determining the decomposition rates of individual organic substrates, a way was found not only to determine static descriptions of an aquatic biotope, but also to make possible a dynamic description of nutrient transfers. The kinetic parameters computed from these measurements are: the maximum uptake velocity (Vmax), the turnover velocity (Tt) and the transport constant (Kt) (Michaelis Constant). The maximum uptake velocity of a substrate can be regarded as the \"heterotrophic capacity\" of the biotope for this substrate. One restriction is, however, that only quickly assimilatable substrates can be used, as for example hexoses, organic acids, amino acids, etc. Since this technique was tested practically only in oligotrophic waters, this study undertakes to investigate its applicability in polluted biotopes and to answer the question whether a decomposition gradient is reflected in the kinetic data. A small eutrophic lake in the eastern section of the Holstein Lake District (350 ha, 9.5 m mean water depth) was chosen as the test biotope. Emptying into the lake was the runoff of a biological water treatment plant with 12,000 population equivalents and without notable business and industry. In a rectangular investigation area at first with 30 sampling stations positioned close to the outlet of the water treatment plant into the lake, kinetic tests were carried out in 48 hour intervals and then compared with bacteriological, chemical and meteorological sample results taken at the same time. Two typical result-blocks are illustrated (Fig. 1-4, 5-8). The first shows four successive test days with periodically strong wind and light to fairly strong but short periods of precipitation. The second block illustrates conditions during a following period of fair weather with only very light wind intensities. The corresponding conditions of the stratification of the drainage water in the investigation area are illustrated by the distribution of coliform organisms. In two other tables the kinetic parameters and individual, dominant data are contrasted numerically...", "contents": "[The use of an enzyme-kinetic-method for bacteriological examinations of heavy polluted surface waters (author's transl)]. The greatest fraction of nutrients dissolved in water are organic substances which are decomposed, transformed and mineralized by heterotrophic microorganisms. For this reason, investigations concerning energy transfer in an aquatic ecosystem, or practical examinations of the pollution load of surface waters, cannot be set up when based exclusively on more or less good counts of microorganisms of the production or destruction group. Above all, it is important for heterotrophic bacteria that productivity parameters for these numerical estimations are also provided. PARSON and STRICKLAND were successful in measuring the uptake of organic material in waters with uptake-kinetic methods; HOBBIE and WRIGHT developed a method for measuring the uptake of marked test-substrates in certain concentration ranges. As a result, for these substrates it was possible to measure the turnover in natural populations. Consequently, after frequent improvement of the technique in determining the decomposition rates of individual organic substrates, a way was found not only to determine static descriptions of an aquatic biotope, but also to make possible a dynamic description of nutrient transfers. The kinetic parameters computed from these measurements are: the maximum uptake velocity (Vmax), the turnover velocity (Tt) and the transport constant (Kt) (Michaelis Constant). The maximum uptake velocity of a substrate can be regarded as the \"heterotrophic capacity\" of the biotope for this substrate. One restriction is, however, that only quickly assimilatable substrates can be used, as for example hexoses, organic acids, amino acids, etc. Since this technique was tested practically only in oligotrophic waters, this study undertakes to investigate its applicability in polluted biotopes and to answer the question whether a decomposition gradient is reflected in the kinetic data. A small eutrophic lake in the eastern section of the Holstein Lake District (350 ha, 9.5 m mean water depth) was chosen as the test biotope. Emptying into the lake was the runoff of a biological water treatment plant with 12,000 population equivalents and without notable business and industry. In a rectangular investigation area at first with 30 sampling stations positioned close to the outlet of the water treatment plant into the lake, kinetic tests were carried out in 48 hour intervals and then compared with bacteriological, chemical and meteorological sample results taken at the same time. Two typical result-blocks are illustrated (Fig. 1-4, 5-8). The first shows four successive test days with periodically strong wind and light to fairly strong but short periods of precipitation. The second block illustrates conditions during a following period of fair weather with only very light wind intensities. The corresponding conditions of the stratification of the drainage water in the investigation area are illustrated by the distribution of coliform organisms. In two other tables the kinetic parameters and individual, dominant data are contrasted numerically...", "PMID": 970024}
{"id": "pubmed23n0032_7711", "title": "[Microcalorimetric investigations in polluted surface waters (author's transl)].", "content": "The hygienic control of polluted surface waters has to be correlated with measurements of the decomposition rate of heterotrophic microbial populations. Starting in 1970 different methods have been tested for this purpose. Uptakekinetic studies of radioactive labelled substrates as well as automated BOD-registrations can not be handled as flow systems. Flow-Microcalorimetry (heat-conduction-principle) could be an easier approach to test the activity of heterotrophic aquatic populations. Probes from a trickling-filter outflow, from an oxidation pond and from a small river were tested simultaneously in a Flow-Microcalorimeter (LKB, 2107, Fig. 1) and a Drop-Microcalorimeter (WADSO, 1974 (fig. 2)) after adding 200 mug/L of Glucose. The resulting voltage/timecurves (Fig. 4,5,6) show a good correlation to the heterotrophic capacity of the probes. The minimal detectable continnous heat effect was 1 muW corresponding to a (relative) activity of 5.6-10(4) bacterial colonies/ml on Difco agar (counts after 48 hours incubation at 22 degrees C incubation temperature). A modification of the Flow-Microcalorimeter using a 5-10 times larger reaction vessel could enable the system to be used also in testing scarcely polluted surface waters and for toxicity tests of surface water samples.", "contents": "[Microcalorimetric investigations in polluted surface waters (author's transl)]. The hygienic control of polluted surface waters has to be correlated with measurements of the decomposition rate of heterotrophic microbial populations. Starting in 1970 different methods have been tested for this purpose. Uptakekinetic studies of radioactive labelled substrates as well as automated BOD-registrations can not be handled as flow systems. Flow-Microcalorimetry (heat-conduction-principle) could be an easier approach to test the activity of heterotrophic aquatic populations. Probes from a trickling-filter outflow, from an oxidation pond and from a small river were tested simultaneously in a Flow-Microcalorimeter (LKB, 2107, Fig. 1) and a Drop-Microcalorimeter (WADSO, 1974 (fig. 2)) after adding 200 mug/L of Glucose. The resulting voltage/timecurves (Fig. 4,5,6) show a good correlation to the heterotrophic capacity of the probes. The minimal detectable continnous heat effect was 1 muW corresponding to a (relative) activity of 5.6-10(4) bacterial colonies/ml on Difco agar (counts after 48 hours incubation at 22 degrees C incubation temperature). A modification of the Flow-Microcalorimeter using a 5-10 times larger reaction vessel could enable the system to be used also in testing scarcely polluted surface waters and for toxicity tests of surface water samples.", "PMID": 970025}
{"id": "pubmed23n0032_7712", "title": "[On N2-fixing clostridia and bacilli from soils (author's transl)].", "content": "In total 30 nitrogen-fixing, saccharolytic Clostridia and 4 nitrogen-fixing bacilli, all freshly isolated from gleyed soils, were screened for sensitivity to 20 antibiotics. The isolates were compared in their sensitivity with 5 type cultures representing the species Clostridium butyricum, C. saccharobutyricum (2 strains) C. multifermentans and C. sporogenes. Generally speaking, both clostridia and bacilli are sensitive to the same antibiotics (Table 2). In addition, the nitrogen-fixing bacilli belonging to Bacillus polymyxa and B. macerans showed sensitivity to neomycin and kanamycin. Except for the species C. tyrobutyricum, none of the various saccharolytic Clostridium species could be distinguished by differences in sensitivity to antibiotics. Differential methods are given in Table 3.", "contents": "[On N2-fixing clostridia and bacilli from soils (author's transl)]. In total 30 nitrogen-fixing, saccharolytic Clostridia and 4 nitrogen-fixing bacilli, all freshly isolated from gleyed soils, were screened for sensitivity to 20 antibiotics. The isolates were compared in their sensitivity with 5 type cultures representing the species Clostridium butyricum, C. saccharobutyricum (2 strains) C. multifermentans and C. sporogenes. Generally speaking, both clostridia and bacilli are sensitive to the same antibiotics (Table 2). In addition, the nitrogen-fixing bacilli belonging to Bacillus polymyxa and B. macerans showed sensitivity to neomycin and kanamycin. Except for the species C. tyrobutyricum, none of the various saccharolytic Clostridium species could be distinguished by differences in sensitivity to antibiotics. Differential methods are given in Table 3.", "PMID": 970026}
{"id": "pubmed23n0032_7713", "title": "[The measurement of fibre concentrations in lung dusts (author's transl)].", "content": "Measurements in ambient air have indicated, that air pollution contains fibrous dusts in various concentration. Moreover it seems to be necessary to get informations of the fiber content in the lungs of men. In this investigation 22 dusts, which were isolated by formamide-methode from lungs, were analysed. Methods of preparation and evaluation of the lung dust of people, which were occupational non-asbestos-exposed, are described. Fiber concentration per mg lung dust lies between 10 X 10(3) and 535 X 10(3). Most of the fibers were shorter than 10 mum. Because of the limitation of the evaluation-technique it was not possible to analyse the fibers with regard to their chemical composition.", "contents": "[The measurement of fibre concentrations in lung dusts (author's transl)]. Measurements in ambient air have indicated, that air pollution contains fibrous dusts in various concentration. Moreover it seems to be necessary to get informations of the fiber content in the lungs of men. In this investigation 22 dusts, which were isolated by formamide-methode from lungs, were analysed. Methods of preparation and evaluation of the lung dust of people, which were occupational non-asbestos-exposed, are described. Fiber concentration per mg lung dust lies between 10 X 10(3) and 535 X 10(3). Most of the fibers were shorter than 10 mum. Because of the limitation of the evaluation-technique it was not possible to analyse the fibers with regard to their chemical composition.", "PMID": 970027}
{"id": "pubmed23n0032_7714", "title": "[The action of the vaccinia virus upon placenta and fetus in revaccinated pregnants (author's transl)].", "content": "We studied 608 antivariolic revaccinated pregnants in spring 1972 at different ages of gestation. 60 presented spontaneous abortus; in 502 cases there has been performed therapeutical abortion, and 46 pregnants revaccinated after 2 1/2-3 months of gestation continued their pregnancy. Pregnancy and birth in revaccinated pregnants which did not abort, evolved without any difference against the witness-sample; the newborns presented in a higher percentage underweight. The absence of the vaccinia-virus and of Guarnieri-inclusions in the examined placentae and embryos as well as the moment of abortion, only after 20 days from the revaccination data, leads to the supposition that the placental lesions do not seem to be produced by the direct action of the virus by its replication, but probably by reactions of hypersensibility of the late type against the alergizin antigens of the antismallpox vaccine. The antivariolic revaccination of the sample of pregnants, showed that the vaccinia virus did not present a malformative action upon the embryo, but in change the abortive action was manifest in indirect proportion to the age of gestation.", "contents": "[The action of the vaccinia virus upon placenta and fetus in revaccinated pregnants (author's transl)]. We studied 608 antivariolic revaccinated pregnants in spring 1972 at different ages of gestation. 60 presented spontaneous abortus; in 502 cases there has been performed therapeutical abortion, and 46 pregnants revaccinated after 2 1/2-3 months of gestation continued their pregnancy. Pregnancy and birth in revaccinated pregnants which did not abort, evolved without any difference against the witness-sample; the newborns presented in a higher percentage underweight. The absence of the vaccinia-virus and of Guarnieri-inclusions in the examined placentae and embryos as well as the moment of abortion, only after 20 days from the revaccination data, leads to the supposition that the placental lesions do not seem to be produced by the direct action of the virus by its replication, but probably by reactions of hypersensibility of the late type against the alergizin antigens of the antismallpox vaccine. The antivariolic revaccination of the sample of pregnants, showed that the vaccinia virus did not present a malformative action upon the embryo, but in change the abortive action was manifest in indirect proportion to the age of gestation.", "PMID": 970028}
{"id": "pubmed23n0032_7715", "title": "Changes in size and shape of chromatin particles after successive removal of histones.", "content": "The preparations of whole chromatin, chromatin selectively depleted of histone f1, depleted of all lysine-rich histones (f1, f2b, f2a2), and DNA was studied by viscosimetric and light scattering methods. The obtained results were used for calculation of the dimensions and packing ratios of DNA for the preparations studied. The packing ratio in whole chromatin is 7.2 and is almost unaffected by selective removal of histone f1 (6.9), but decreases on successive removal of the remaining four histones, the decrease being dependent more on the quantity than the kind of the dissociated histones.", "contents": "Changes in size and shape of chromatin particles after successive removal of histones. The preparations of whole chromatin, chromatin selectively depleted of histone f1, depleted of all lysine-rich histones (f1, f2b, f2a2), and DNA was studied by viscosimetric and light scattering methods. The obtained results were used for calculation of the dimensions and packing ratios of DNA for the preparations studied. The packing ratio in whole chromatin is 7.2 and is almost unaffected by selective removal of histone f1 (6.9), but decreases on successive removal of the remaining four histones, the decrease being dependent more on the quantity than the kind of the dissociated histones.", "PMID": 970029}
{"id": "pubmed23n0032_7716", "title": "The effect of calcium restriction in the diet of calcium transport in rat small intestine.", "content": "1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calcium transport in duodenum was highly stimulated after 3 days, then gradually declined and after 28 days almost disappeared. In proximal jejunum it was the highest between 7 and 21 days. In distal ileum, the transport appeared after 3 days and increased progressively until 21 days, but markedly decreased at the 28-th day. The normal pattern of calcium transport was reestablished on refeeding the animals with standard diet.", "contents": "The effect of calcium restriction in the diet of calcium transport in rat small intestine. 1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calcium transport in duodenum was highly stimulated after 3 days, then gradually declined and after 28 days almost disappeared. In proximal jejunum it was the highest between 7 and 21 days. In distal ileum, the transport appeared after 3 days and increased progressively until 21 days, but markedly decreased at the 28-th day. The normal pattern of calcium transport was reestablished on refeeding the animals with standard diet.", "PMID": 970030}
{"id": "pubmed23n0032_7717", "title": "Preparation and properties of tRNA from human placenta.", "content": "1. The tRNA preparation obtained from human placenta by phenol extraction and DEAE-cellulose chromatography was homogeneous on ultracentrifugation and showed high amino acid acceptor activity. 2. The analysis of the isoaccepting tRNA by reversed-phase chromatography (RPC-5 system) showed the presence of 5 fractions for glycine and leucine, 3 for tyrosine, alanine and valine, 2 for arginine and 1 for phenylalanine.", "contents": "Preparation and properties of tRNA from human placenta. 1. The tRNA preparation obtained from human placenta by phenol extraction and DEAE-cellulose chromatography was homogeneous on ultracentrifugation and showed high amino acid acceptor activity. 2. The analysis of the isoaccepting tRNA by reversed-phase chromatography (RPC-5 system) showed the presence of 5 fractions for glycine and leucine, 3 for tyrosine, alanine and valine, 2 for arginine and 1 for phenylalanine.", "PMID": 970031}
{"id": "pubmed23n0032_7718", "title": "Non-histone chromatin proteins during maturation of avian erythroid cells.", "content": "1. In chicken erythroid cells (erythroblast, reticulocyte and erythrocyte) maturation was accompanied by a decrease in the content of non-histone chromatin proteins. 2. Phenol-soluble non-histone chromatin proteins (phosphoproteins) from the three cell populations studied, showed differences in the behavior on sodium dodecyl sulphate polyacrylamide-gel electrophoresis, and isoelectric focusing. Phosphoprotein of immature cells had a higher content of fractions of about 86 000 and 23 000 daltons than the phosphoproteins of erythrocytes.", "contents": "Non-histone chromatin proteins during maturation of avian erythroid cells. 1. In chicken erythroid cells (erythroblast, reticulocyte and erythrocyte) maturation was accompanied by a decrease in the content of non-histone chromatin proteins. 2. Phenol-soluble non-histone chromatin proteins (phosphoproteins) from the three cell populations studied, showed differences in the behavior on sodium dodecyl sulphate polyacrylamide-gel electrophoresis, and isoelectric focusing. Phosphoprotein of immature cells had a higher content of fractions of about 86 000 and 23 000 daltons than the phosphoproteins of erythrocytes.", "PMID": 970032}
{"id": "pubmed23n0032_7719", "title": "Potassium-dependent thermal sensibility of AMP-deaminase from rabbit skeletal muscle.", "content": "1. AMP-deaminase (AMP-aminohydrolase, EC 3.5.4.6) from rabbit skeletal muscle showed sigmoid-shaped plots of velocity versus substrate concentration at four temperatures tested between 15 degrees and 35 degrees C. In the presence of 20 mM-KCl, the plot was sigmoid only at 30 degrees C and became hyperbolic at the other temperatures tested. In the presence of 150 mM-KCl the plots were hyperbolic at all the temperatures applied. 2. The Km value depended on temperature and concentration of KCl, whereas Vmax was the same for the 20 mM- and 150 mM-KCl-activated enzyme. 3. The value of enthalpy of the enzyme-substrate complex formation was the same for both the 20 mM- and 150-mM-KCl-activated enzyme at lower temperature range (less than 38 degrees C), whereas at higher temperatures (greater than 38 degrees C) this value was much more negative in the presence of 20 mM-KCl than of 150 mM-KCl.", "contents": "Potassium-dependent thermal sensibility of AMP-deaminase from rabbit skeletal muscle. 1. AMP-deaminase (AMP-aminohydrolase, EC 3.5.4.6) from rabbit skeletal muscle showed sigmoid-shaped plots of velocity versus substrate concentration at four temperatures tested between 15 degrees and 35 degrees C. In the presence of 20 mM-KCl, the plot was sigmoid only at 30 degrees C and became hyperbolic at the other temperatures tested. In the presence of 150 mM-KCl the plots were hyperbolic at all the temperatures applied. 2. The Km value depended on temperature and concentration of KCl, whereas Vmax was the same for the 20 mM- and 150 mM-KCl-activated enzyme. 3. The value of enthalpy of the enzyme-substrate complex formation was the same for both the 20 mM- and 150-mM-KCl-activated enzyme at lower temperature range (less than 38 degrees C), whereas at higher temperatures (greater than 38 degrees C) this value was much more negative in the presence of 20 mM-KCl than of 150 mM-KCl.", "PMID": 970034}
{"id": "pubmed23n0032_7720", "title": "Regulation of progesterone biosynthesis in human placental mitochondria by Krebs cycle metabolites.", "content": "1. 2-Oxoglutarate, succinate, fumarate, malate and citrate, cis-aconitate and isocitrate stimulate conversion of cholesterol to progesterone in human placental mitochondria. 2. The stimulatory effect of dicarboxylic and tricarboxylic acids depends on the activity of malate dehydrogenase (decarboxylating) (NADP+) (EC 1.1.1.40) and isocitrate dehydrogenase (NADP+) (EC 1.1.1.42), respectively.", "contents": "Regulation of progesterone biosynthesis in human placental mitochondria by Krebs cycle metabolites. 1. 2-Oxoglutarate, succinate, fumarate, malate and citrate, cis-aconitate and isocitrate stimulate conversion of cholesterol to progesterone in human placental mitochondria. 2. The stimulatory effect of dicarboxylic and tricarboxylic acids depends on the activity of malate dehydrogenase (decarboxylating) (NADP+) (EC 1.1.1.40) and isocitrate dehydrogenase (NADP+) (EC 1.1.1.42), respectively.", "PMID": 970033}
{"id": "pubmed23n0032_7721", "title": "Glutamate metabolism in rat kidney mitochondria.", "content": "1. Kidney cortex mitochondria did not swell in ammonium or potassium salts of glutamate even in the presence of valinomycin or 2,4-dinitrophenol. Aminooxyacetate diminished the reduction of nicotinamide nucleotides in intact mitochondria in the presence of glutamate. 2. Transamination with oxaloacetate appeared to be the main pathway of glutamate metabolism in isolated rat kidney cortex mitochondria under various metabolic conditions. Ammonia formation was negligible. The gamma-aminobutyrate pathway was found to be of almost no importance.", "contents": "Glutamate metabolism in rat kidney mitochondria. 1. Kidney cortex mitochondria did not swell in ammonium or potassium salts of glutamate even in the presence of valinomycin or 2,4-dinitrophenol. Aminooxyacetate diminished the reduction of nicotinamide nucleotides in intact mitochondria in the presence of glutamate. 2. Transamination with oxaloacetate appeared to be the main pathway of glutamate metabolism in isolated rat kidney cortex mitochondria under various metabolic conditions. Ammonia formation was negligible. The gamma-aminobutyrate pathway was found to be of almost no importance.", "PMID": 970035}
{"id": "pubmed23n0032_7722", "title": "Effect of (-)hydroxycitrate on the activities of ATP citrate lyase and the enzymes of acetyl-CoA metabolism in rat brain.", "content": "1. (-)Hyrdoxycitrate is a potent inhibitor of ATP citrate (pro-3S)lyase (EC 4.1.3.8) from rat brain, the inhibition being uncompetitive with respect to MgATP2-and competitive with citrate (Ki 0.8 muM). 2. The rate of oxygen consumption by rat brain synaptosomes and the activities of fatty acid synthetase, carnitine acetyltransferase, glucose-6-phosphate dehydrogenase and acetyl-CoA-synthetase are not affected by (-)hydroxycitrate. 3. (-)Hydroxycitrate inhibits the activities of isocitrate dehydrogenase, malate dehydrogenase (decarboxylating) and aconitate hydratase at millimolar concentrations.", "contents": "Effect of (-)hydroxycitrate on the activities of ATP citrate lyase and the enzymes of acetyl-CoA metabolism in rat brain. 1. (-)Hyrdoxycitrate is a potent inhibitor of ATP citrate (pro-3S)lyase (EC 4.1.3.8) from rat brain, the inhibition being uncompetitive with respect to MgATP2-and competitive with citrate (Ki 0.8 muM). 2. The rate of oxygen consumption by rat brain synaptosomes and the activities of fatty acid synthetase, carnitine acetyltransferase, glucose-6-phosphate dehydrogenase and acetyl-CoA-synthetase are not affected by (-)hydroxycitrate. 3. (-)Hydroxycitrate inhibits the activities of isocitrate dehydrogenase, malate dehydrogenase (decarboxylating) and aconitate hydratase at millimolar concentrations.", "PMID": 970036}
{"id": "pubmed23n0032_7723", "title": "Transfer of cholesterol and lecithin between erythrocytes and serum in vitro.", "content": "1. In human blood incubated in vitro, the transfer of free cholesterol and lecithin from erythrocytes to serum was not related to glycolytic activity in erythrocytes or esterification of cholesterol in serum. 2. The stability of free cholesterol concentration in serum was dependent on the activity of lecithin:cholesterol acyltransferase (EC 2.3.1.43), and the stability of lecithin concentration in erythrocytes, on glycolytic activity.", "contents": "Transfer of cholesterol and lecithin between erythrocytes and serum in vitro. 1. In human blood incubated in vitro, the transfer of free cholesterol and lecithin from erythrocytes to serum was not related to glycolytic activity in erythrocytes or esterification of cholesterol in serum. 2. The stability of free cholesterol concentration in serum was dependent on the activity of lecithin:cholesterol acyltransferase (EC 2.3.1.43), and the stability of lecithin concentration in erythrocytes, on glycolytic activity.", "PMID": 970037}
{"id": "pubmed23n0032_7724", "title": "RNA polymerases I and II in germinating wheat embryo.", "content": "1. The RNA polymerase I was practically absent in the resting embryos and appeared several hours after the beginning of imbibition, whereas the level of polymerase II was high in the resting embryos and did not increase significantly during the imbibition phase. 2. Incorporation in vivo of [14C]valine into polymerase I and II indicated that the synthesis of RNA polymerase I is initiated in germinating embryos much earlier than that of RNA polymerase II. 3. It is suggested that RNA polymerase II is stored in resting wheat embryos to support mRNA synthesis at the onset of germination, whereas the RNA polymerase I activity appears at a further stage of germination.", "contents": "RNA polymerases I and II in germinating wheat embryo. 1. The RNA polymerase I was practically absent in the resting embryos and appeared several hours after the beginning of imbibition, whereas the level of polymerase II was high in the resting embryos and did not increase significantly during the imbibition phase. 2. Incorporation in vivo of [14C]valine into polymerase I and II indicated that the synthesis of RNA polymerase I is initiated in germinating embryos much earlier than that of RNA polymerase II. 3. It is suggested that RNA polymerase II is stored in resting wheat embryos to support mRNA synthesis at the onset of germination, whereas the RNA polymerase I activity appears at a further stage of germination.", "PMID": 970038}
{"id": "pubmed23n0032_7725", "title": "Stimulation of citrate oxidation and transport in human placental mitochondria by L-malate.", "content": "1. Citrate was oxidized by human placental mitochondria at a rate half that for isocitrate, and cis-aconitate at a rate by 20% lower as with isocitrate. The apparent Km values for these substrates were similar. 2. Oxidation of citrate was stimulated by L-malate but the stimulation was abolished by benzene 1,2,3-tricarboxylate and butylmalonate. 3. Citrate uptake by placental mitochondria was inhibited by benzene 1,2,3--tricarboxylate, and raised specifically by L-malate. Stimulation by fumarate and succinate was found to be due to conversion of these substrates to L-malate. 4. It is concluded that human placental mitochondria contain malate-stimulated tricarboxylate carrier system.", "contents": "Stimulation of citrate oxidation and transport in human placental mitochondria by L-malate. 1. Citrate was oxidized by human placental mitochondria at a rate half that for isocitrate, and cis-aconitate at a rate by 20% lower as with isocitrate. The apparent Km values for these substrates were similar. 2. Oxidation of citrate was stimulated by L-malate but the stimulation was abolished by benzene 1,2,3-tricarboxylate and butylmalonate. 3. Citrate uptake by placental mitochondria was inhibited by benzene 1,2,3--tricarboxylate, and raised specifically by L-malate. Stimulation by fumarate and succinate was found to be due to conversion of these substrates to L-malate. 4. It is concluded that human placental mitochondria contain malate-stimulated tricarboxylate carrier system.", "PMID": 970039}
{"id": "pubmed23n0032_7726", "title": "[Intracellular protein breakdown. VIII. The use of double-labeled proteins as substrates].", "content": "Double-labeled proteins from rat liver cytosol (14C in long-lived, 3H in short-lived proteins after in-vivo-labeling) are used as substrates for unlabeled proteinases in vitro. Differences in the degradation rates of short-lived and long-lived proteins in vitro by different proteinases and after addition of different effectors allow conclusions concerning their importance for the in-vivo-turnover of substrate proteins. The main activity (greater than 90%) of soluble-lysosomal proteinases at pH 6,1 and pH 6,9 is caused by thiolproteinases, which degrade preferentially short-lived cytosol proteins. These proteinases are inhibited by leupeptin. Autolysis of double-labeled cell fractions shows a remarkably faster breakdown of short-lived substrate proteins only in the soluble part of lysosomes. Microsomal fractions degrade in vitro preferentially long-lived substrate proteins.", "contents": "[Intracellular protein breakdown. VIII. The use of double-labeled proteins as substrates]. Double-labeled proteins from rat liver cytosol (14C in long-lived, 3H in short-lived proteins after in-vivo-labeling) are used as substrates for unlabeled proteinases in vitro. Differences in the degradation rates of short-lived and long-lived proteins in vitro by different proteinases and after addition of different effectors allow conclusions concerning their importance for the in-vivo-turnover of substrate proteins. The main activity (greater than 90%) of soluble-lysosomal proteinases at pH 6,1 and pH 6,9 is caused by thiolproteinases, which degrade preferentially short-lived cytosol proteins. These proteinases are inhibited by leupeptin. Autolysis of double-labeled cell fractions shows a remarkably faster breakdown of short-lived substrate proteins only in the soluble part of lysosomes. Microsomal fractions degrade in vitro preferentially long-lived substrate proteins.", "PMID": 970040}
{"id": "pubmed23n0032_7727", "title": "[Organ and subcellular distribution of transmidinase in man and rats].", "content": "The transamidinase capacity of human organs is consistently higher than those of rats. Both species show the highest activity in pancreas and slightly smaller in kidneys. The human liver is less active while the enzyme is not present at all in rat liver. Very low enzyme activities have been found in spleen, heart and skeleton muscle. During cell fractionation of kidney and pancreas a remarkable loss of enzyme activity occurs. Thus the transamidinase in man and rat is equally found in cytosol, mitochondria and nuclei. In the native cell the enzyme is probably localized mainly in the mitochondria.", "contents": "[Organ and subcellular distribution of transmidinase in man and rats]. The transamidinase capacity of human organs is consistently higher than those of rats. Both species show the highest activity in pancreas and slightly smaller in kidneys. The human liver is less active while the enzyme is not present at all in rat liver. Very low enzyme activities have been found in spleen, heart and skeleton muscle. During cell fractionation of kidney and pancreas a remarkable loss of enzyme activity occurs. Thus the transamidinase in man and rat is equally found in cytosol, mitochondria and nuclei. In the native cell the enzyme is probably localized mainly in the mitochondria.", "PMID": 970041}
{"id": "pubmed23n0032_7728", "title": "[Influence of uncoupling agents on the capacity of transamidinase in kidney and pancreas].", "content": "It was found in white rats that hyperthyreosis (0.5% thyreoidin in dry food), respectively 2,4-dinitrophenol (30 mg/kg body weight/day i. p.), deficiency of the vitamins K and E or complete fasting increase the content of creatine in muscle, plasma and urine, but reduce the concentration of creatinine. Simultaneously the elevated creatine level of blood causes a repression of transamidinase in kidneys and pancreas. The common mode of action of the mentioned agents is seen in a decoupling of the oxidative phosphorylation, and in an insufficient availability of ATP, respectively.", "contents": "[Influence of uncoupling agents on the capacity of transamidinase in kidney and pancreas]. It was found in white rats that hyperthyreosis (0.5% thyreoidin in dry food), respectively 2,4-dinitrophenol (30 mg/kg body weight/day i. p.), deficiency of the vitamins K and E or complete fasting increase the content of creatine in muscle, plasma and urine, but reduce the concentration of creatinine. Simultaneously the elevated creatine level of blood causes a repression of transamidinase in kidneys and pancreas. The common mode of action of the mentioned agents is seen in a decoupling of the oxidative phosphorylation, and in an insufficient availability of ATP, respectively.", "PMID": 970042}
{"id": "pubmed23n0032_7729", "title": "[Activation of leucine aminopeptidase under hydrogen bond cleaving conditions].", "content": "Cleavage of hydrogen bonds by urea, guanidinium chloride or elevated temperatures causes a reversible activation of leucine aminopeptidase. The activation is similar to that caused by Mg2+ ions. This means that preincubation is required and that a 10-fold or more activated enzyme is inhibited by 50 mM cyanide to 20 per cent while a C1-ion-activated enzyme like the nonactivated enzyme is inhibited to 90 per cent. Blockage of the free SH-groups reduces the response time of the activation. The free SH-groups are involved in an essential intermediate step of the activation.", "contents": "[Activation of leucine aminopeptidase under hydrogen bond cleaving conditions]. Cleavage of hydrogen bonds by urea, guanidinium chloride or elevated temperatures causes a reversible activation of leucine aminopeptidase. The activation is similar to that caused by Mg2+ ions. This means that preincubation is required and that a 10-fold or more activated enzyme is inhibited by 50 mM cyanide to 20 per cent while a C1-ion-activated enzyme like the nonactivated enzyme is inhibited to 90 per cent. Blockage of the free SH-groups reduces the response time of the activation. The free SH-groups are involved in an essential intermediate step of the activation.", "PMID": 970043}
{"id": "pubmed23n0032_7730", "title": "Bovine lens leucine aminopeptidase. A study of possible roles for the thiol groups in holo- and apoenzyme.", "content": "The apoenzyme of leucine aminopeptidase has to be prepared either under inert conditions or in the presence of 2-thioethanol. Due to the removal of essential zinc ions, buried -SH groups are exposed. These -SH groups show a higher reactivity than the six -SH groups accessible in the native zinc enzyme. During the incubation of the zinc enzyme with guanidine hydrochloride, up to 36 -SH groups are titratble in dependence on the concentration of guanidine hydrochloride. The -SH groups can be distinguished by their reactivity towards Ellman's reagent and can be differentiated into four classes with respect to their function.", "contents": "Bovine lens leucine aminopeptidase. A study of possible roles for the thiol groups in holo- and apoenzyme. The apoenzyme of leucine aminopeptidase has to be prepared either under inert conditions or in the presence of 2-thioethanol. Due to the removal of essential zinc ions, buried -SH groups are exposed. These -SH groups show a higher reactivity than the six -SH groups accessible in the native zinc enzyme. During the incubation of the zinc enzyme with guanidine hydrochloride, up to 36 -SH groups are titratble in dependence on the concentration of guanidine hydrochloride. The -SH groups can be distinguished by their reactivity towards Ellman's reagent and can be differentiated into four classes with respect to their function.", "PMID": 970044}
{"id": "pubmed23n0032_7731", "title": "[Affinity labeling of leucine aminopeptidase with new substrate analog inhibitors].", "content": "Substrate analogous compounds differing both in type and reactivity (diazonium group, chloro and mo ethyl ketone groups) and in the position of the reactive group at the inhibitor molecule were studied for their adequacy for affinity labelling of leucine amino-peptidase. The chloro methyl ketone derivatives of amino acids with free alpha-amino group are competitive inhibitors. Also, the more reactive bromine compound PheCh2br (Ki 1.2 mM) compared with the PheCH2C1 (Ki 0.3 mM) fails to give an irreversible inactivation of leucine amino-peptidase. The dipeptide derivatives Leu-PheCH2C1 and Phe-LeuCH2C1 also inhibit the enzyme activity up to 65%, but they are split at the peptide bond under reactivation of the enzyme. p-Diazophenylalanine methylketone Phe (pN+/2)CH3 and the two dipeptides Phe(pN+/2)-Phe and Phe(pN+/2)-Phe(pN+/2) with N-terminal diazonium groups afford an irreversible inactivation of leucine aminopeptidase in a time- and concentration-dependent reaction. The Phe-Phe(pN+/2) reactive only C-terminally, is less effective; the inhibition is partly reversible. The inactivation is strongly reduced by the competitive inhibitor Thr(but)-Phe-Pro. These effects are discussed with regard to the specific site of attack of the inhibitors in the active binding centre of leucine aminopeptidase. The synthesis of the three parasubstituted amino derivatives of phenylalanyl-phenylalanine Phe(pNH2)-Phe, Phe-Phe(pNH2) and Phe(pNH2)-Phe(pNH2) and their selective conversion to the respective diazonium peptides, retaining the aliphatic alpha-amino group, are discussed.", "contents": "[Affinity labeling of leucine aminopeptidase with new substrate analog inhibitors]. Substrate analogous compounds differing both in type and reactivity (diazonium group, chloro and mo ethyl ketone groups) and in the position of the reactive group at the inhibitor molecule were studied for their adequacy for affinity labelling of leucine amino-peptidase. The chloro methyl ketone derivatives of amino acids with free alpha-amino group are competitive inhibitors. Also, the more reactive bromine compound PheCh2br (Ki 1.2 mM) compared with the PheCH2C1 (Ki 0.3 mM) fails to give an irreversible inactivation of leucine amino-peptidase. The dipeptide derivatives Leu-PheCH2C1 and Phe-LeuCH2C1 also inhibit the enzyme activity up to 65%, but they are split at the peptide bond under reactivation of the enzyme. p-Diazophenylalanine methylketone Phe (pN+/2)CH3 and the two dipeptides Phe(pN+/2)-Phe and Phe(pN+/2)-Phe(pN+/2) with N-terminal diazonium groups afford an irreversible inactivation of leucine aminopeptidase in a time- and concentration-dependent reaction. The Phe-Phe(pN+/2) reactive only C-terminally, is less effective; the inhibition is partly reversible. The inactivation is strongly reduced by the competitive inhibitor Thr(but)-Phe-Pro. These effects are discussed with regard to the specific site of attack of the inhibitors in the active binding centre of leucine aminopeptidase. The synthesis of the three parasubstituted amino derivatives of phenylalanyl-phenylalanine Phe(pNH2)-Phe, Phe-Phe(pNH2) and Phe(pNH2)-Phe(pNH2) and their selective conversion to the respective diazonium peptides, retaining the aliphatic alpha-amino group, are discussed.", "PMID": 970045}
{"id": "pubmed23n0032_7732", "title": "[Isolation and characterization of a microsomal arylaminopeptidase from rat kidney].", "content": "The isolation and characterization of a microsomal arylaminopeptidase from rat kidney is reported. By treatment of a microsomal arylaminopeptidase-phosphatase-complex with trypsin and subsequent gel filtration of the solubilized proteins on Sepharose 6B a electrophoretic homogeneous arylaminopeptidase was obtained (yield, 3%; enrichment, 900 times). The following properties of the purified enzyme were determined: 1. Molecular weight: 182000 (gel filtration on Sepharose 6B) to 192000 (SDS-polyacrylamide gel electrophoresis). 2. Subunit structure: In the presence of 6 M guanidine - HC1 + 1% BETA-mercaptoethanol the enzyme dissociates into subunits (MW 46700, ESTIMATED BY SDS gel electrophoresis method). 3. Isoelectric point: 4,71 (agarose gel electrophoresis method). 4. UV characteristics: E 280nm/E260NM=1.3. 5. Substrate specifity: optimal substrates L-alanyl derivatives (anilide, beta-naphthyl amide, p-nitroanilide, 4-(phenylazo)-phenylamide and hydrazide). Among these compounds the anilide derivative was hydrolyzed most rapidly. Furthermore, di- and tripeptides, especially L-methionyl-L-leucine, were also split. No hydrolysis was observed with hemoglobin (pH 4.5 and 7.5) and amino acid- or peptide-ester substrates. 6. Optimal pH: 7.5 +/- 0,1; optimal temperature: 45 to 50 degrees C. 7. The enzyme has no transamidation activity with L-alanyl amide both as aminoacyl donator and -acceptor. 8. Influence of effectors: Heavy metal ions (Ni2+, Cd2+, Cu2+, Zn2+), chelating agents (EDTA, o-phenanthroline) and puromycin inhibit the enzyme significantly. SH-group reagents are without any influence. 9. L-alanyl-L-alanyl-4 (phenylazo)-phenylamide, a dipeptide aryl aminopeptidase substrate, is hydrolyzed by the purified enzyme preparation according to a consecutive or step by step mechanism.", "contents": "[Isolation and characterization of a microsomal arylaminopeptidase from rat kidney]. The isolation and characterization of a microsomal arylaminopeptidase from rat kidney is reported. By treatment of a microsomal arylaminopeptidase-phosphatase-complex with trypsin and subsequent gel filtration of the solubilized proteins on Sepharose 6B a electrophoretic homogeneous arylaminopeptidase was obtained (yield, 3%; enrichment, 900 times). The following properties of the purified enzyme were determined: 1. Molecular weight: 182000 (gel filtration on Sepharose 6B) to 192000 (SDS-polyacrylamide gel electrophoresis). 2. Subunit structure: In the presence of 6 M guanidine - HC1 + 1% BETA-mercaptoethanol the enzyme dissociates into subunits (MW 46700, ESTIMATED BY SDS gel electrophoresis method). 3. Isoelectric point: 4,71 (agarose gel electrophoresis method). 4. UV characteristics: E 280nm/E260NM=1.3. 5. Substrate specifity: optimal substrates L-alanyl derivatives (anilide, beta-naphthyl amide, p-nitroanilide, 4-(phenylazo)-phenylamide and hydrazide). Among these compounds the anilide derivative was hydrolyzed most rapidly. Furthermore, di- and tripeptides, especially L-methionyl-L-leucine, were also split. No hydrolysis was observed with hemoglobin (pH 4.5 and 7.5) and amino acid- or peptide-ester substrates. 6. Optimal pH: 7.5 +/- 0,1; optimal temperature: 45 to 50 degrees C. 7. The enzyme has no transamidation activity with L-alanyl amide both as aminoacyl donator and -acceptor. 8. Influence of effectors: Heavy metal ions (Ni2+, Cd2+, Cu2+, Zn2+), chelating agents (EDTA, o-phenanthroline) and puromycin inhibit the enzyme significantly. SH-group reagents are without any influence. 9. L-alanyl-L-alanyl-4 (phenylazo)-phenylamide, a dipeptide aryl aminopeptidase substrate, is hydrolyzed by the purified enzyme preparation according to a consecutive or step by step mechanism.", "PMID": 970046}
{"id": "pubmed23n0032_7733", "title": "[CHEMICAL OSCILLATIONS IN THE ONE-ENZYME REACTOR].", "content": "The theory of nonlinear oscillations was applied to a special dynamic system, the continuous stirred tank enzyme reactor (CSTR), in order to find out the enzyme mechanisms which may lead to the appearence of chemical oscillations in the one-enzyme reactors. In the main, the isothermic reactor without diffusion limitation was studied. The possibilities of a mathematical analysis of periodic processes in single catalyst beads and one-enzyme column reactors with diffusion limitation are briefly discussed.", "contents": "[CHEMICAL OSCILLATIONS IN THE ONE-ENZYME REACTOR]. The theory of nonlinear oscillations was applied to a special dynamic system, the continuous stirred tank enzyme reactor (CSTR), in order to find out the enzyme mechanisms which may lead to the appearence of chemical oscillations in the one-enzyme reactors. In the main, the isothermic reactor without diffusion limitation was studied. The possibilities of a mathematical analysis of periodic processes in single catalyst beads and one-enzyme column reactors with diffusion limitation are briefly discussed.", "PMID": 970047}
{"id": "pubmed23n0032_7734", "title": "Ionic interactions in eukaryotic ribosomes: splitting of the subunits of rat liver ribosomes by treatment with monovalent cations.", "content": "Proteins of rat liver ribosomal subunits were successively split off by stepwise treatment of the particles with various concentrations of LiC1 (0.4--1.0 M) or KC1 (0.6--1.5 M). Split proteins and core particle proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The LiC1 concentrations were estimated at which the individual proteins are split off. The proteins of 40S and 60S subunits were classified in 4 and 5 groups, respectively, according to their splitting behavior. The effect of LiC1 and KC1 is similar, but higher concentrations of KC1 than of LiC1 are required to obtain comparable split protein patterns. Evidence for structural changes of the core particles during the split procedures was obtained.", "contents": "Ionic interactions in eukaryotic ribosomes: splitting of the subunits of rat liver ribosomes by treatment with monovalent cations. Proteins of rat liver ribosomal subunits were successively split off by stepwise treatment of the particles with various concentrations of LiC1 (0.4--1.0 M) or KC1 (0.6--1.5 M). Split proteins and core particle proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The LiC1 concentrations were estimated at which the individual proteins are split off. The proteins of 40S and 60S subunits were classified in 4 and 5 groups, respectively, according to their splitting behavior. The effect of LiC1 and KC1 is similar, but higher concentrations of KC1 than of LiC1 are required to obtain comparable split protein patterns. Evidence for structural changes of the core particles during the split procedures was obtained.", "PMID": 970048}
{"id": "pubmed23n0032_7735", "title": "[Insulin and glucagon secretion in the isolated perfused rat pancreas].", "content": "For the study of insulin and glucagon secretion at the isolated perfused rat pancreas are used, as a rule, synthetic perfusion media that have to meet two requirements: They have to ensure the biological function of the perfused organ for 2 hrs, and must not interfere with radioimmunological insulin and glucagon determination. An appropriate medium is the Krebs-Ringer bicarbonate buffer at pH 7.4 with addition of 4% dextran (MG 75000) and 0.5% human serum albumin; it allows one to produce, after permanent glucose stimulation, a biphasic insulin secretion. Infusion media based on gelatine or polyvinylpyrrolidone proved inadequate for these studies.", "contents": "[Insulin and glucagon secretion in the isolated perfused rat pancreas]. For the study of insulin and glucagon secretion at the isolated perfused rat pancreas are used, as a rule, synthetic perfusion media that have to meet two requirements: They have to ensure the biological function of the perfused organ for 2 hrs, and must not interfere with radioimmunological insulin and glucagon determination. An appropriate medium is the Krebs-Ringer bicarbonate buffer at pH 7.4 with addition of 4% dextran (MG 75000) and 0.5% human serum albumin; it allows one to produce, after permanent glucose stimulation, a biphasic insulin secretion. Infusion media based on gelatine or polyvinylpyrrolidone proved inadequate for these studies.", "PMID": 970049}
{"id": "pubmed23n0032_7736", "title": "[Isolation and characterization of rubredoxin from Acinetobacter calcoaceticus].", "content": "Acinetobacter calcoaceticus growing on long-chain n-alkanes contains a soluble iron-sulfur protein, which corresponds in its properties to a rubredoxin. It was prepared from the 50000 X g supernatant of ultrasonically treated cells using ion exchange chromatography on DEAE cellulose and gel filtration on Sephadex G-75. The isolated protein is pure electrophoretically, but yields two bands corresponding to molecular weights of 6000 and 12000 respectively. A content of 11 acidic against 6 basic amino acids is in line with the acidic character of the protein. The absence of acid-labile sulfur, content of 4 cysteine residues and one iron atom per polypeptide chain and the typical absorption maxima at gamma = 280, 380, and 490 nm exclude the presence of a ferredoxin. Involvement of the rubredoxin in the alkane hydroxylation is discussed.", "contents": "[Isolation and characterization of rubredoxin from Acinetobacter calcoaceticus]. Acinetobacter calcoaceticus growing on long-chain n-alkanes contains a soluble iron-sulfur protein, which corresponds in its properties to a rubredoxin. It was prepared from the 50000 X g supernatant of ultrasonically treated cells using ion exchange chromatography on DEAE cellulose and gel filtration on Sephadex G-75. The isolated protein is pure electrophoretically, but yields two bands corresponding to molecular weights of 6000 and 12000 respectively. A content of 11 acidic against 6 basic amino acids is in line with the acidic character of the protein. The absence of acid-labile sulfur, content of 4 cysteine residues and one iron atom per polypeptide chain and the typical absorption maxima at gamma = 280, 380, and 490 nm exclude the presence of a ferredoxin. Involvement of the rubredoxin in the alkane hydroxylation is discussed.", "PMID": 970050}
{"id": "pubmed23n0032_7737", "title": "[System analysis of the multi-oscillatory functional order in the circadian and ultradian frequency range and its monitoring value for load effects, exemplified by a varying light-dark regimen in the intensive rearing of sheep].", "content": "As part of the experiments to influence the annual rhythmicity of sexual reproduction, various artificial light-dark regimens were tested for their systemic effects. From the polygraphic registration of several functions, such as food ingestion, rumination, movement, cardiac and respiratory rhythm were obtained biorhythmometric system-analytic parameters of the multi-oscillatory functional order. Under well-balanced light-dark conditions (LD 13.5:10.5), a high intra- and interparametric degree of functional coupling of 80% is obtained, while under extreme conditions (LD 22:2) this value amounts to 39%, which is equivalent to a disorganized time structure and disturbed neuro-vegetative multi-oscillatory functional order. The significance of these or analogously contrived studies on environmental problems under human social conditions is pointed out.", "contents": "[System analysis of the multi-oscillatory functional order in the circadian and ultradian frequency range and its monitoring value for load effects, exemplified by a varying light-dark regimen in the intensive rearing of sheep]. As part of the experiments to influence the annual rhythmicity of sexual reproduction, various artificial light-dark regimens were tested for their systemic effects. From the polygraphic registration of several functions, such as food ingestion, rumination, movement, cardiac and respiratory rhythm were obtained biorhythmometric system-analytic parameters of the multi-oscillatory functional order. Under well-balanced light-dark conditions (LD 13.5:10.5), a high intra- and interparametric degree of functional coupling of 80% is obtained, while under extreme conditions (LD 22:2) this value amounts to 39%, which is equivalent to a disorganized time structure and disturbed neuro-vegetative multi-oscillatory functional order. The significance of these or analogously contrived studies on environmental problems under human social conditions is pointed out.", "PMID": 970051}
{"id": "pubmed23n0032_7738", "title": "Anticholinesterase action of 3-diethylaminophenyl-N-methyl-carbamate methiodide in vitro and in vivo.", "content": "The inhibitory effect of 3-diethylaminophenyl-N-methylcarbamate methiodide on rat brain acetylcholinesterase and horse plasma butyrylcholinesterase was studied in vitro. This quaternary carbamate is a more potent inhibitor of acetylcholinesterase than butyrylcholinesterase. Complete inhibition of acetylcholinesterase may be achieved without any inhibition of butyrylcholinesterase, i.e. that 3-diethylaminophenyl-N-methylcarbamate methiodide is a selective inhibitor of acetylcholinesterase. The inhibitory effect of different doses of this compound on plasma and liver butyrylcholinesterase and erythrocyte, brain, heart and diaphragm acetylcholinesterase of the rat was studied in vivo. With increasing doses of carbamate the inhibition of the enzymes in the plasma, erythrocytes, liver, heart, and diaphragm increased while the brain acetylcholinesterase was unaffected. The toxic action of carbamate studied was preferably due to acetylcholin esterase inhibition in the peripheral nervous system and this compound cannot penetrate the blood-brain barrier.", "contents": "Anticholinesterase action of 3-diethylaminophenyl-N-methyl-carbamate methiodide in vitro and in vivo. The inhibitory effect of 3-diethylaminophenyl-N-methylcarbamate methiodide on rat brain acetylcholinesterase and horse plasma butyrylcholinesterase was studied in vitro. This quaternary carbamate is a more potent inhibitor of acetylcholinesterase than butyrylcholinesterase. Complete inhibition of acetylcholinesterase may be achieved without any inhibition of butyrylcholinesterase, i.e. that 3-diethylaminophenyl-N-methylcarbamate methiodide is a selective inhibitor of acetylcholinesterase. The inhibitory effect of different doses of this compound on plasma and liver butyrylcholinesterase and erythrocyte, brain, heart and diaphragm acetylcholinesterase of the rat was studied in vivo. With increasing doses of carbamate the inhibition of the enzymes in the plasma, erythrocytes, liver, heart, and diaphragm increased while the brain acetylcholinesterase was unaffected. The toxic action of carbamate studied was preferably due to acetylcholin esterase inhibition in the peripheral nervous system and this compound cannot penetrate the blood-brain barrier.", "PMID": 970052}
{"id": "pubmed23n0032_7739", "title": "[Influence of strophanthin and 2,4-dinitrophenol to the scopolamine-evoked acetylcholine release in rat cortex].", "content": "In rats narcotized by urethan we investigated the effect of 2,4-dinitrophenol and ouabain with regard to the cortical release of acetylcholine stimulated by scopolamine and to the acetylcholine content of telencephalon changed in that way. Dinitrophenol increased the liberating effect of scopolamine. In the presence of dinitrophenol and scopolamine ouabain reduced acetylcholine synthesis resulting in an strong decrease of acetylcholine content on persistent diminished increase of acetylcholine release.", "contents": "[Influence of strophanthin and 2,4-dinitrophenol to the scopolamine-evoked acetylcholine release in rat cortex]. In rats narcotized by urethan we investigated the effect of 2,4-dinitrophenol and ouabain with regard to the cortical release of acetylcholine stimulated by scopolamine and to the acetylcholine content of telencephalon changed in that way. Dinitrophenol increased the liberating effect of scopolamine. In the presence of dinitrophenol and scopolamine ouabain reduced acetylcholine synthesis resulting in an strong decrease of acetylcholine content on persistent diminished increase of acetylcholine release.", "PMID": 970053}
{"id": "pubmed23n0032_7740", "title": "[Angiotensin-induced hypertension in pigs].", "content": "In pigs receiving up to 14 days daily injections of depot angiotensin, repeated daily bloody measurements of the blood pressure at the carotid artery proved unsuitable because of ensueing septic processes, in contrast to the unbloody measurement at the caudal artery. The depot angiotensin, despite the use of preparations with varying content of angiotensin-II and carrier solution, caused immediately after the injections a very strong maximal rise in systolic and diastolic blood pressure that occurred also with longer periods of the experiment; this rise gradually diminished in the course of the day but remained mostly in the hypertonic range and showed daily initial values above normal. The pig proved to be much more sensitive to angiotensin than the rat, for instance. Despite the proved vasoconstricting action of angiotensin, light-microscopic alterations at the vessels, kidneys, and the myocardium could not be observed. Part of the arterioles showed an increased wall thickness in animals killed on the 8th and 15th day of the experiment. The differences in the pathomorphological and blood pressure behaviour from the rat are discussed. The first experimental results qualify the pig as an experimental animal for comparative hypertension studies.", "contents": "[Angiotensin-induced hypertension in pigs]. In pigs receiving up to 14 days daily injections of depot angiotensin, repeated daily bloody measurements of the blood pressure at the carotid artery proved unsuitable because of ensueing septic processes, in contrast to the unbloody measurement at the caudal artery. The depot angiotensin, despite the use of preparations with varying content of angiotensin-II and carrier solution, caused immediately after the injections a very strong maximal rise in systolic and diastolic blood pressure that occurred also with longer periods of the experiment; this rise gradually diminished in the course of the day but remained mostly in the hypertonic range and showed daily initial values above normal. The pig proved to be much more sensitive to angiotensin than the rat, for instance. Despite the proved vasoconstricting action of angiotensin, light-microscopic alterations at the vessels, kidneys, and the myocardium could not be observed. Part of the arterioles showed an increased wall thickness in animals killed on the 8th and 15th day of the experiment. The differences in the pathomorphological and blood pressure behaviour from the rat are discussed. The first experimental results qualify the pig as an experimental animal for comparative hypertension studies.", "PMID": 970054}
{"id": "pubmed23n0032_7741", "title": "The effect of 35SO2 binding by ammonia on sulfur incorporation into rat tissues.", "content": "Sulfur metabolism was traced in experimental investigations on rats, and the importance of the method of sulfur dioxide neutralized by ammonia was estimated. The animals were exposed to 35SO2 by means of inhalation, and radioactivity in several organs at different periods of exposure was traced. Apart from that, autoradiographic investigations were carried out in order to show the incorporation of radioactive sulfur into particular elements of the cell in different tissues. Elimination of isotope with urine and feces at different periods of time after exposure was also determined. It was found that the use of ammonia to neutralize sulfur dioxide in the air decreased the amount of sulfur which could enter the organism as a result of breathing air contaminated by SO2.", "contents": "The effect of 35SO2 binding by ammonia on sulfur incorporation into rat tissues. Sulfur metabolism was traced in experimental investigations on rats, and the importance of the method of sulfur dioxide neutralized by ammonia was estimated. The animals were exposed to 35SO2 by means of inhalation, and radioactivity in several organs at different periods of exposure was traced. Apart from that, autoradiographic investigations were carried out in order to show the incorporation of radioactive sulfur into particular elements of the cell in different tissues. Elimination of isotope with urine and feces at different periods of time after exposure was also determined. It was found that the use of ammonia to neutralize sulfur dioxide in the air decreased the amount of sulfur which could enter the organism as a result of breathing air contaminated by SO2.", "PMID": 970055}
{"id": "pubmed23n0032_7742", "title": "Lymphocyte-dependent antibodies in uveitis.", "content": "Lymphocyte-dependent antibodies were revealed in the serum of patients suffering from uveitis of various aetiologies. The serum was incubated with normal uveal tissue and the binding of non-immune human lymphocytes was investigated. In three cases of sympathetic ophthalmitis the lymphocytes accumulated around the melanine granules, while in another 17 patients with uveitis cases the lymphocytes accumulated around the capillaries. Uveal tissue incubated with control sera failed to bound lymphocytes. The lymphocytic infiltration in certain cases of chronic uveitis suggested the role of lymphocyte-mediating antibodies in the aetiology of these cases.", "contents": "Lymphocyte-dependent antibodies in uveitis. Lymphocyte-dependent antibodies were revealed in the serum of patients suffering from uveitis of various aetiologies. The serum was incubated with normal uveal tissue and the binding of non-immune human lymphocytes was investigated. In three cases of sympathetic ophthalmitis the lymphocytes accumulated around the melanine granules, while in another 17 patients with uveitis cases the lymphocytes accumulated around the capillaries. Uveal tissue incubated with control sera failed to bound lymphocytes. The lymphocytic infiltration in certain cases of chronic uveitis suggested the role of lymphocyte-mediating antibodies in the aetiology of these cases.", "PMID": 970058}
{"id": "pubmed23n0032_7743", "title": "[Diagnostic angiography in mediastinal and pulmonary diseases].", "content": "The angiographic methods employed in chest surgery are reviewed and their value is demonstrated on practical examples. In certain cases angiography is the only means of arriving to the correct diagnosis and its result often determines the course of therapy. Angiographic methods are claimed to be equivalent to the classical diagnostic procedures applied in pulmonology and their wide-spread use is recommended.", "contents": "[Diagnostic angiography in mediastinal and pulmonary diseases]. The angiographic methods employed in chest surgery are reviewed and their value is demonstrated on practical examples. In certain cases angiography is the only means of arriving to the correct diagnosis and its result often determines the course of therapy. Angiographic methods are claimed to be equivalent to the classical diagnostic procedures applied in pulmonology and their wide-spread use is recommended.", "PMID": 970059}
{"id": "pubmed23n0032_7744", "title": "Radiology preceding reconstructive coronary operations.", "content": "In connection with the fact that lesions of the coronary artery can be approached only by coronarography, the possibilities of coronarography are discussed. The problems arising are, 1. the relationship between prognosis and the degree to which the coronaries are affected; 2. how to follow the degenerative lesion from the first event up to complete obstruction; 3. the relationship between coronarographic finding and clinical symptoms; and 4. why is it essential to be acquainted with the changed conditions of coronary circulation. A coronarographic finding taking into consideration the above points will play an important role when it has to be decided who should be subjected and to what type of operation improving the coronary circulation.", "contents": "Radiology preceding reconstructive coronary operations. In connection with the fact that lesions of the coronary artery can be approached only by coronarography, the possibilities of coronarography are discussed. The problems arising are, 1. the relationship between prognosis and the degree to which the coronaries are affected; 2. how to follow the degenerative lesion from the first event up to complete obstruction; 3. the relationship between coronarographic finding and clinical symptoms; and 4. why is it essential to be acquainted with the changed conditions of coronary circulation. A coronarographic finding taking into consideration the above points will play an important role when it has to be decided who should be subjected and to what type of operation improving the coronary circulation.", "PMID": 970060}
{"id": "pubmed23n0032_7745", "title": "Laser stimulation of wound healing.", "content": "Serum complement activity, immunoglobulin levels and the circulating auto-antibodies were studied in the course of laser treatment of 20 cases of crural ulcer. After temporary changes a normalization of the humoral immune response was observed in the healing cases, while in the stagnating ones opposite trend was manifest. In none of the groups were detected circulating auto-antibodies against the investigated antigens.", "contents": "Laser stimulation of wound healing. Serum complement activity, immunoglobulin levels and the circulating auto-antibodies were studied in the course of laser treatment of 20 cases of crural ulcer. After temporary changes a normalization of the humoral immune response was observed in the healing cases, while in the stagnating ones opposite trend was manifest. In none of the groups were detected circulating auto-antibodies against the investigated antigens.", "PMID": 970061}
{"id": "pubmed23n0032_7746", "title": "Viability of extracorporeally perfused dog livers.", "content": "The possibility of structural and functional preservation of the isolated liver has been studied in dog experiments. A sterile liver preparation left in situ was worked out, having practically no ischaemic period, except during perfusion. Nevertheless, well-defined lesions (perivascular and interstitial oedema, dissociated contraction of the smooth muscle elements of the veins, cellula accumulation in the sinusoids) appeared, accompanied by a gradual reduction (microcirculatory disorders, enzyme derailment) of the function of hepatocytes.", "contents": "Viability of extracorporeally perfused dog livers. The possibility of structural and functional preservation of the isolated liver has been studied in dog experiments. A sterile liver preparation left in situ was worked out, having practically no ischaemic period, except during perfusion. Nevertheless, well-defined lesions (perivascular and interstitial oedema, dissociated contraction of the smooth muscle elements of the veins, cellula accumulation in the sinusoids) appeared, accompanied by a gradual reduction (microcirculatory disorders, enzyme derailment) of the function of hepatocytes.", "PMID": 970062}
{"id": "pubmed23n0032_7747", "title": "[Serum enzyme activities in intestinal obstruction].", "content": "In patients with intestinal obstruction, nine of the enzymes usually studied in liver diagnostics were determined before and after operation. Intestinal obstruction causes reversible changes in the liver and in hepatic metabolism; they normalize after relief of the obstruction.", "contents": "[Serum enzyme activities in intestinal obstruction]. In patients with intestinal obstruction, nine of the enzymes usually studied in liver diagnostics were determined before and after operation. Intestinal obstruction causes reversible changes in the liver and in hepatic metabolism; they normalize after relief of the obstruction.", "PMID": 970064}
{"id": "pubmed23n0032_7748", "title": "Effect of denervation on the longitudinal growth of tubular bones.", "content": "The effect of sciatic nerve lesion on the longitudinal growth of tubular bones of the affected extremity has been studied in adolescent rabbits. For the observation of circulation in the bones, intravenously injected Sr85Cl2 solution was used. A retardation of longitudinal growth of the tibia and of the femur on the operated side was noted. Radioactivity in the bones of the operated extremity was always lower than on the control side. For the retarded longitudinal growth, a circulatory disturbance may have been responsible.", "contents": "Effect of denervation on the longitudinal growth of tubular bones. The effect of sciatic nerve lesion on the longitudinal growth of tubular bones of the affected extremity has been studied in adolescent rabbits. For the observation of circulation in the bones, intravenously injected Sr85Cl2 solution was used. A retardation of longitudinal growth of the tibia and of the femur on the operated side was noted. Radioactivity in the bones of the operated extremity was always lower than on the control side. For the retarded longitudinal growth, a circulatory disturbance may have been responsible.", "PMID": 970065}
{"id": "pubmed23n0032_7749", "title": "[Gonadotropin levels and spermatogenesis in infertile patients].", "content": "165 STERILE MALE PATIENTS UNDERWENT SEMEN EXAMINATIONS AND SERUM GONADOTROPINS DOSAGE. In 47 of these patients a testicular biopsy was preformed. The results of the statistical analysis showed a significant correlation between the sperm count and the FSH in addition to a close and precise relation between the initial stages of the spermatogenesis and FSH. No correlation was found to exist in regard to the LH. The Authors finally showed their interpertation of such data, with the possibility of different prognosis in subjects having severe oligozoospermia, but with a bioptic picture which did or did not present damage at the spermatid maturation stage.", "contents": "[Gonadotropin levels and spermatogenesis in infertile patients]. 165 STERILE MALE PATIENTS UNDERWENT SEMEN EXAMINATIONS AND SERUM GONADOTROPINS DOSAGE. In 47 of these patients a testicular biopsy was preformed. The results of the statistical analysis showed a significant correlation between the sperm count and the FSH in addition to a close and precise relation between the initial stages of the spermatogenesis and FSH. No correlation was found to exist in regard to the LH. The Authors finally showed their interpertation of such data, with the possibility of different prognosis in subjects having severe oligozoospermia, but with a bioptic picture which did or did not present damage at the spermatid maturation stage.", "PMID": 970072}
{"id": "pubmed23n0032_7750", "title": "In vivo and in vitro experiments on relationships between PGA1 and glucose utilization.", "content": "The in vivo and in vitro effects of PGA1 on glucose utilization were investigated in normal rats and in rats with alloxan-diabetes (50 mg/kg i.v. administered 48 hrs before experiment). The animals were divided into two groups. The first group -- which included both normal and diabetic animals -- was submitted to an IVGIT after a 12-h fast and during a sodium chloride infusion. In the second group -- which equally included normal and diabetic rats -- the same GTT was performed during a sodium chloride infusion in which PGA1 had been diluted, so that a dose of 0.5 g/kg/min was administered. This dose is devoid of any effect on cardiovascular activity. For in vitro experiments, glucose utilization was studied in the rat diaphragm incubated with insulin (200 muU/ml) and PGA1 (10 and 100 ng): results demonstrated that PGA1 enhances the insulin effect on glucose utilization and the enhancement is dose-dependent. The same results were observed also in the in vivo experiments: in normal rats PGA1 really improves glucose utilization without any interference with insulin secretion from B-cells. On the other hand, PGA1 has no effect on this utilization in diabetic rats. From our experiments it can therefore be concluded that PGA1 improves glucose utilization, showing a synergic action with the increased quantity of insulin secreted in response to a glucose load. No effect is noted when insulin secretion from B-cells is reduced or absent.", "contents": "In vivo and in vitro experiments on relationships between PGA1 and glucose utilization. The in vivo and in vitro effects of PGA1 on glucose utilization were investigated in normal rats and in rats with alloxan-diabetes (50 mg/kg i.v. administered 48 hrs before experiment). The animals were divided into two groups. The first group -- which included both normal and diabetic animals -- was submitted to an IVGIT after a 12-h fast and during a sodium chloride infusion. In the second group -- which equally included normal and diabetic rats -- the same GTT was performed during a sodium chloride infusion in which PGA1 had been diluted, so that a dose of 0.5 g/kg/min was administered. This dose is devoid of any effect on cardiovascular activity. For in vitro experiments, glucose utilization was studied in the rat diaphragm incubated with insulin (200 muU/ml) and PGA1 (10 and 100 ng): results demonstrated that PGA1 enhances the insulin effect on glucose utilization and the enhancement is dose-dependent. The same results were observed also in the in vivo experiments: in normal rats PGA1 really improves glucose utilization without any interference with insulin secretion from B-cells. On the other hand, PGA1 has no effect on this utilization in diabetic rats. From our experiments it can therefore be concluded that PGA1 improves glucose utilization, showing a synergic action with the increased quantity of insulin secreted in response to a glucose load. No effect is noted when insulin secretion from B-cells is reduced or absent.", "PMID": 970068}
{"id": "pubmed23n0032_7751", "title": "The glycerylphosphorylcholine content of the rat epididymis after injecting alpha-chlorohydrin and ligating the vasa efferentia.", "content": "The concentration of GPC in the 4 segments (initial segment, caput, corpus and cauda) of the rat epididymis was examined at various times (4 hours. 2, 5, 10 and 20 days) following injection of a lesion-forming dose (90 mg/kg body weight) of alpha-chlorohydrin and bilateral ligation of the vasa efferentia. There was a reduction in the overall GPC concentration of the epididymis 2 days after both treatments and a further decrease until 20 days when a slight increase occurred. It is suggested that a lesion-forming dose of alpha-chlorohydrin, like ligation of the vasa efferentia, could inhibit the synthesis and/or release of GPC within the epididymis by blocking off androgen supply in the testicular fluid.", "contents": "The glycerylphosphorylcholine content of the rat epididymis after injecting alpha-chlorohydrin and ligating the vasa efferentia. The concentration of GPC in the 4 segments (initial segment, caput, corpus and cauda) of the rat epididymis was examined at various times (4 hours. 2, 5, 10 and 20 days) following injection of a lesion-forming dose (90 mg/kg body weight) of alpha-chlorohydrin and bilateral ligation of the vasa efferentia. There was a reduction in the overall GPC concentration of the epididymis 2 days after both treatments and a further decrease until 20 days when a slight increase occurred. It is suggested that a lesion-forming dose of alpha-chlorohydrin, like ligation of the vasa efferentia, could inhibit the synthesis and/or release of GPC within the epididymis by blocking off androgen supply in the testicular fluid.", "PMID": 970074}
{"id": "pubmed23n0032_7752", "title": "Reliability of copper reduction methods for quantitation of glucosuria.", "content": "In the present study we evaluated the reliability of the \"2-drop\" Clinitest method in determining the 24-h glucose spill of a diabetic patient. The urine glucose content over the 33 day study period was measured with a Beckman glucose analyzer that employed a glucose oxidase method. There was no statistical difference between the glucose content determined by the two methods. There was a mean error of +4.4 +/- 3.6% between the values obtained with the \"2-drop\" method and values obtained with glucose analyzer. The mean coefficient of variation for determining the glucose content of urine with the \"2 drop\" method was 19%. In a group of 10 normal volunteers we found that there was a falsely elevated glucose value in urines with osmolality of less than 295 mosm/kg when the \"5-drop\" method was used. The \"2-drop\" Clinitest and the Beckman analyzer could accurately determine glucose concentration even in these dilute urines. The \"2-drop\" Clinitest method is a useful and reasonably reliable method for evaluationg 24-h glucosuria.", "contents": "Reliability of copper reduction methods for quantitation of glucosuria. In the present study we evaluated the reliability of the \"2-drop\" Clinitest method in determining the 24-h glucose spill of a diabetic patient. The urine glucose content over the 33 day study period was measured with a Beckman glucose analyzer that employed a glucose oxidase method. There was no statistical difference between the glucose content determined by the two methods. There was a mean error of +4.4 +/- 3.6% between the values obtained with the \"2-drop\" method and values obtained with glucose analyzer. The mean coefficient of variation for determining the glucose content of urine with the \"2 drop\" method was 19%. In a group of 10 normal volunteers we found that there was a falsely elevated glucose value in urines with osmolality of less than 295 mosm/kg when the \"5-drop\" method was used. The \"2-drop\" Clinitest and the Beckman analyzer could accurately determine glucose concentration even in these dilute urines. The \"2-drop\" Clinitest method is a useful and reasonably reliable method for evaluationg 24-h glucosuria.", "PMID": 970069}
{"id": "pubmed23n0032_7753", "title": "Riboflavin deficiency in children with diabetes mellitus.", "content": "Twenty-four insulin-dependent diabetic children and 114 normal control children, all between the ages of 6 to 16 years, were investigated for riboflavin deficiency. The method used was a measurement of erythrocyte glutathione reductase activity and the results were expressed as the activity coefficient (AC). None of the children had received vitamin supplementation. The percentage of diabetic children with riboflavin deficiency was 4 fold greater than in non diabetics. Supplementation with daily oral riboflavin quickly returned all AC values to normal.", "contents": "Riboflavin deficiency in children with diabetes mellitus. Twenty-four insulin-dependent diabetic children and 114 normal control children, all between the ages of 6 to 16 years, were investigated for riboflavin deficiency. The method used was a measurement of erythrocyte glutathione reductase activity and the results were expressed as the activity coefficient (AC). None of the children had received vitamin supplementation. The percentage of diabetic children with riboflavin deficiency was 4 fold greater than in non diabetics. Supplementation with daily oral riboflavin quickly returned all AC values to normal.", "PMID": 970067}
{"id": "pubmed23n0032_7754", "title": "The Somogyi phenomenon. A short review.", "content": "The Somogyi phenomenon or effect is a paradoxical situation of insulin-induced post-hypoglycemic hyperglycemia. The historical aspects of this phenomenon and the subsequent hypotheses and controversy are reviewed. The clinical situation is explained, with regard to its recognition, management and importance as an etiological factor in \"brittle\" diabetes. Hormone immunoassay techniques at present show human growth hormone (HGH) to be the major consequence of insulin-induced hypoglycemia leading to post-hypoglycemia glucose intolerance, but further studies will probably show glucagon to have a major role.", "contents": "The Somogyi phenomenon. A short review. The Somogyi phenomenon or effect is a paradoxical situation of insulin-induced post-hypoglycemic hyperglycemia. The historical aspects of this phenomenon and the subsequent hypotheses and controversy are reviewed. The clinical situation is explained, with regard to its recognition, management and importance as an etiological factor in \"brittle\" diabetes. Hormone immunoassay techniques at present show human growth hormone (HGH) to be the major consequence of insulin-induced hypoglycemia leading to post-hypoglycemia glucose intolerance, but further studies will probably show glucagon to have a major role.", "PMID": 970071}
{"id": "pubmed23n0032_7755", "title": "Some peculiarities of the glucoregulatory response to glucose infusion in the rat.", "content": "The glucoregulatory response to the i.v. infusion of different doses of glucose and glucose plus insulin was studied in anesthetized rats by using the primed constant infusion of glucose-2-3H. Infusion of glucose at the rate of 10 mg/kg/min induced a rise of about 100% in blood glucose, while the hepatic release of glucose showed only a small and transient decrease. A proportional increase of glycemia and glucose utilization (Rd) was observed without any appreciable change in the metabolic clearance rate (MCR) of glucose; a two-fold increase in plasma insulin was recorded at all times. In the group of rats receiving 20 mg/kg/min of glucose, changes in the above parameters were slightly greater; MCR showed a moderate increment in spite of the six-fold rise of plasma insulin. Finally, the influsion of large doses of insulin together with 20 mg/kg/min of glucose resulted in complete cessation of glucose release by the liver and in a remarkable increase of Rd and MCR. These results suggest a poor adaptability of the glucoregulatory system of the rat in response to glucose infusion as compared to other mammalian species.", "contents": "Some peculiarities of the glucoregulatory response to glucose infusion in the rat. The glucoregulatory response to the i.v. infusion of different doses of glucose and glucose plus insulin was studied in anesthetized rats by using the primed constant infusion of glucose-2-3H. Infusion of glucose at the rate of 10 mg/kg/min induced a rise of about 100% in blood glucose, while the hepatic release of glucose showed only a small and transient decrease. A proportional increase of glycemia and glucose utilization (Rd) was observed without any appreciable change in the metabolic clearance rate (MCR) of glucose; a two-fold increase in plasma insulin was recorded at all times. In the group of rats receiving 20 mg/kg/min of glucose, changes in the above parameters were slightly greater; MCR showed a moderate increment in spite of the six-fold rise of plasma insulin. Finally, the influsion of large doses of insulin together with 20 mg/kg/min of glucose resulted in complete cessation of glucose release by the liver and in a remarkable increase of Rd and MCR. These results suggest a poor adaptability of the glucoregulatory system of the rat in response to glucose infusion as compared to other mammalian species.", "PMID": 970066}
{"id": "pubmed23n0032_7756", "title": "Juvenile diabetic cheiroarthropathy.", "content": "A peculiar involvement of the interphalangeal joints of both hands with palmar flexion of the fingers has been observed in 11 insulin-treated, nonrheumatoid, juvenile diabetics. The onset of diabetes occurred between 1 and 12 years of age. Painless deformities of the fingers with progressive stiffness and impaired extension started 4 to 10 years later. One patient complained of articular pain and swelling. X-ray and circulatory changes were absent or minimal. Prepubertal patients showed delayed puberty and stunted growth, adult patients had normal sexual development. Rheumatic or rheumatoid signs were absent. Electromyography showed minor abnormalities of the motor units, normal or subnormal motor nerve conduction velocity, increased median nerve terminal latency, in the absence of muscular atrophy or thickening of palmar tendons. Vibratory sensitivity was impaired in 1 subject. Juvenile cheiroarthropathy is associated with: a) early onset and poor control of diabetes; b) stunted growth; c) hepatomegaly; d) delayed puberty; e) long standing administration of insulin. The articular changes are distinct from previously known forms of \"diabetic hand\", such as atrophic neuropathy, osteoarthropathy, Dupuytren's contracture, carpal tunnel syndrome.", "contents": "Juvenile diabetic cheiroarthropathy. A peculiar involvement of the interphalangeal joints of both hands with palmar flexion of the fingers has been observed in 11 insulin-treated, nonrheumatoid, juvenile diabetics. The onset of diabetes occurred between 1 and 12 years of age. Painless deformities of the fingers with progressive stiffness and impaired extension started 4 to 10 years later. One patient complained of articular pain and swelling. X-ray and circulatory changes were absent or minimal. Prepubertal patients showed delayed puberty and stunted growth, adult patients had normal sexual development. Rheumatic or rheumatoid signs were absent. Electromyography showed minor abnormalities of the motor units, normal or subnormal motor nerve conduction velocity, increased median nerve terminal latency, in the absence of muscular atrophy or thickening of palmar tendons. Vibratory sensitivity was impaired in 1 subject. Juvenile cheiroarthropathy is associated with: a) early onset and poor control of diabetes; b) stunted growth; c) hepatomegaly; d) delayed puberty; e) long standing administration of insulin. The articular changes are distinct from previously known forms of \"diabetic hand\", such as atrophic neuropathy, osteoarthropathy, Dupuytren's contracture, carpal tunnel syndrome.", "PMID": 970070}
{"id": "pubmed23n0032_7757", "title": "Acute liver failure due to temporary hepatic ischemia in the pig.", "content": "The model of temporary complete liver ischemia was investigated in 60 pigs in order to produce a disease which resembles liver failure in man. The interval of time leading to death in every animal differed in a wide range. A number of biochemical alterations was of no value as for the prognosis of the animal under investigation. The ammonium in the peripheral blood is elevated during shunting of portal blood around the liver only. Microscopic examinations demonstrated that the damage produced is limited to the hepatic tissue. The lesions were less when ischemia was produced after a longer interval after the operation. The role of processes of regeneration and reparation of liver tissue is discussed.", "contents": "Acute liver failure due to temporary hepatic ischemia in the pig. The model of temporary complete liver ischemia was investigated in 60 pigs in order to produce a disease which resembles liver failure in man. The interval of time leading to death in every animal differed in a wide range. A number of biochemical alterations was of no value as for the prognosis of the animal under investigation. The ammonium in the peripheral blood is elevated during shunting of portal blood around the liver only. Microscopic examinations demonstrated that the damage produced is limited to the hepatic tissue. The lesions were less when ischemia was produced after a longer interval after the operation. The role of processes of regeneration and reparation of liver tissue is discussed.", "PMID": 970080}
{"id": "pubmed23n0032_7758", "title": "About the efficiency of the duodenal drainage with the double balloon tube technique using polyethylene glycol as duodenal marker.", "content": "Using polyethylene glycol (PEG) as a duodenal marker substance, we studied the efficiency of the duodenal drainage with the double balloon tube technique employing three various tubes: a) the three-lumen double balloon tube according to Bartelheimer, b) a modified double balloon tube with more valid preconditions for the combination of duodenal marker and double balloon tube technique, and c) this modified tube after removal of the distal balloon. The results with this valid marker technique represent the first experimental proof that the duodenal juice can be collected in an approximately quantitative way by the described double balloon tube techniques. They therefore confirm the importance of these tubes for all those studies in which a quantitative drainage is desired as much as possible.", "contents": "About the efficiency of the duodenal drainage with the double balloon tube technique using polyethylene glycol as duodenal marker. Using polyethylene glycol (PEG) as a duodenal marker substance, we studied the efficiency of the duodenal drainage with the double balloon tube technique employing three various tubes: a) the three-lumen double balloon tube according to Bartelheimer, b) a modified double balloon tube with more valid preconditions for the combination of duodenal marker and double balloon tube technique, and c) this modified tube after removal of the distal balloon. The results with this valid marker technique represent the first experimental proof that the duodenal juice can be collected in an approximately quantitative way by the described double balloon tube techniques. They therefore confirm the importance of these tubes for all those studies in which a quantitative drainage is desired as much as possible.", "PMID": 970081}
{"id": "pubmed23n0032_7759", "title": "Possible role of beta-glucuronidase in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis.", "content": "Histochemical demonstration of beta-glucuronidase was carried out in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis. Greater enzyme activity was particularly discernible in the stratum germinativum of the skin of the normal and fully regenerated tail. During regeneration most of the cellular elements of the blastema and the differentiating regenerate exhibited enzyme activity. Distribution of the enzyme beta-glucuronidase is correlated with the high degree of cellular proliferation. The role of the enzyme in the catabolism of mucopolysaccharides is also implicated during the wound-healing phase. In the full-grown regenerate the intensity and distribution of the enzyme activity were found to be the same as those observed in the normal tail.", "contents": "Possible role of beta-glucuronidase in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis. Histochemical demonstration of beta-glucuronidase was carried out in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis. Greater enzyme activity was particularly discernible in the stratum germinativum of the skin of the normal and fully regenerated tail. During regeneration most of the cellular elements of the blastema and the differentiating regenerate exhibited enzyme activity. Distribution of the enzyme beta-glucuronidase is correlated with the high degree of cellular proliferation. The role of the enzyme in the catabolism of mucopolysaccharides is also implicated during the wound-healing phase. In the full-grown regenerate the intensity and distribution of the enzyme activity were found to be the same as those observed in the normal tail.", "PMID": 970088}
{"id": "pubmed23n0032_7760", "title": "Effect of pregnenolone-16alpha-carbonitrile (PCN) on rat liver.", "content": "One time i.p. injections of 5 -20 mg Pregnenolone-16alpha-carbonitrile (PCN) effects the mitotic activity of rat liver cells during a time period of 24 hours to be 20 times higher than the normal mitotic rate. This, however, does not result in an measurable increase of cell numbers and cell volumes. Furthermore, total liver DNA, RNA, protein and dry-weight remain unchanged. The injection of 7 times 10 mg PCN also results in the elevation of the mitotic rate. Return of the elevated mitotic rate to base level takes 18 days from the time the last injection of PCN was administered. During this time the number and the average volume of liver cells increases by 28% and 30%, respectively. The dry weight of liver and tetraploid nuclei increase in number, whereas RNA- and protein content remains unchanged. All parameters have reached the base line after 4 - 6 weeks following the last injection of PCN. No histological changes were observed after multiple doses of PCN that cause hyperplasia and hypertrophy of liver tissue. Hypertrophy of liver cells follows the observed hyperplasia and therefore is not expected to be the cause of the hyperplastic processes.", "contents": "Effect of pregnenolone-16alpha-carbonitrile (PCN) on rat liver. One time i.p. injections of 5 -20 mg Pregnenolone-16alpha-carbonitrile (PCN) effects the mitotic activity of rat liver cells during a time period of 24 hours to be 20 times higher than the normal mitotic rate. This, however, does not result in an measurable increase of cell numbers and cell volumes. Furthermore, total liver DNA, RNA, protein and dry-weight remain unchanged. The injection of 7 times 10 mg PCN also results in the elevation of the mitotic rate. Return of the elevated mitotic rate to base level takes 18 days from the time the last injection of PCN was administered. During this time the number and the average volume of liver cells increases by 28% and 30%, respectively. The dry weight of liver and tetraploid nuclei increase in number, whereas RNA- and protein content remains unchanged. All parameters have reached the base line after 4 - 6 weeks following the last injection of PCN. No histological changes were observed after multiple doses of PCN that cause hyperplasia and hypertrophy of liver tissue. Hypertrophy of liver cells follows the observed hyperplasia and therefore is not expected to be the cause of the hyperplastic processes.", "PMID": 970082}
{"id": "pubmed23n0032_7761", "title": "Treatment of extrahepatic occlusive jaundice with activated charcoal hemoperfusion in dogs.", "content": "To find the feasibility of treatment for congenital bile duct atresia, we studied the usefullness of extracorporeal hemoperfusion over activated charcoal in canine obstructive jaundice. One, three and five weeks after ligation and disection of common bile duct in 5 dogs we performed the hemoperfusion over activated charcoal extracorporeally (group 3). In this animals we examined hematological and blood coagulation studies, serum electrolyte levels, kidney function tests and liver chemistries. As control in 5 animals we carried out after sham operation the perfusion without common bile duct ligation (group 2) and in 5 animals only common bile duct ligation without perfusion (group 1). In the liver chemistries we found 2 weeks after 2nd and 3rd perfusion (5 and 7 weeks after bile duct ligation) lower levels of serum bilirubin, GOT, GPT and SDH in treated group than in non-treated jaundiced animals. It suggest the effectiveness of hemoperfusion with activated charcoal in the treatment of occlusive jaundice. There were no alteration in the hematological studies, serum electrolyte levels and kidney function tests. PT and PTT was prolonged in the jaundiced animals there were no significant differences with and without hemoperfusion.", "contents": "Treatment of extrahepatic occlusive jaundice with activated charcoal hemoperfusion in dogs. To find the feasibility of treatment for congenital bile duct atresia, we studied the usefullness of extracorporeal hemoperfusion over activated charcoal in canine obstructive jaundice. One, three and five weeks after ligation and disection of common bile duct in 5 dogs we performed the hemoperfusion over activated charcoal extracorporeally (group 3). In this animals we examined hematological and blood coagulation studies, serum electrolyte levels, kidney function tests and liver chemistries. As control in 5 animals we carried out after sham operation the perfusion without common bile duct ligation (group 2) and in 5 animals only common bile duct ligation without perfusion (group 1). In the liver chemistries we found 2 weeks after 2nd and 3rd perfusion (5 and 7 weeks after bile duct ligation) lower levels of serum bilirubin, GOT, GPT and SDH in treated group than in non-treated jaundiced animals. It suggest the effectiveness of hemoperfusion with activated charcoal in the treatment of occlusive jaundice. There were no alteration in the hematological studies, serum electrolyte levels and kidney function tests. PT and PTT was prolonged in the jaundiced animals there were no significant differences with and without hemoperfusion.", "PMID": 970083}
{"id": "pubmed23n0032_7762", "title": "Quantitative studies on the mouse facial nerve trunk distal to the geniculate ganglion. An electron-microscopic study.", "content": "A fiber count analysis was performed with the electron microscope on the facial nerve trunk distal to the geniculate ganglion in seven mice. On an average, 4,836 (93%) of the total nerve fibers (5,201) were myelinated and 328 (6.3%) unmyelinated. The large part of the nerve consisted mostly of large myelinated fibers (large-fiber group). The small part of the nerve consisted of small myelinated and unmyelinated fibers (small-fiber group). The fiber constitution of the small-fiber group in the nerve was more like that in the intermediate nerve than that of the small-fiber group in the motor root of the facial nerve. Thus, it is postulated that the nerve fibers of the small-fiber group in the facial nerve trunk distal to the geniculate ganglion may be derived from the intermediate nerve, and that the nerve fibers of the small-fiber group in the motor root of facial nerve may course to the greater petrosal nerve.", "contents": "Quantitative studies on the mouse facial nerve trunk distal to the geniculate ganglion. An electron-microscopic study. A fiber count analysis was performed with the electron microscope on the facial nerve trunk distal to the geniculate ganglion in seven mice. On an average, 4,836 (93%) of the total nerve fibers (5,201) were myelinated and 328 (6.3%) unmyelinated. The large part of the nerve consisted mostly of large myelinated fibers (large-fiber group). The small part of the nerve consisted of small myelinated and unmyelinated fibers (small-fiber group). The fiber constitution of the small-fiber group in the nerve was more like that in the intermediate nerve than that of the small-fiber group in the motor root of the facial nerve. Thus, it is postulated that the nerve fibers of the small-fiber group in the facial nerve trunk distal to the geniculate ganglion may be derived from the intermediate nerve, and that the nerve fibers of the small-fiber group in the motor root of facial nerve may course to the greater petrosal nerve.", "PMID": 970089}
{"id": "pubmed23n0032_7763", "title": "[The surface-coat of Rathke's pouch and the neural tube in embryonic mice].", "content": "Mouse embryos, gestation days 10 and 11, were fixed by the method of LUFT (ruthenium red). At the ultrastructural level, luminal epithelial cell membranes of Rathke's pouch and the neural tube were coated with a continuous, electron-dense layer of 100-700 A thickness. The biological property of this surface coat is discussed.", "contents": "[The surface-coat of Rathke's pouch and the neural tube in embryonic mice]. Mouse embryos, gestation days 10 and 11, were fixed by the method of LUFT (ruthenium red). At the ultrastructural level, luminal epithelial cell membranes of Rathke's pouch and the neural tube were coated with a continuous, electron-dense layer of 100-700 A thickness. The biological property of this surface coat is discussed.", "PMID": 970090}
{"id": "pubmed23n0032_7764", "title": "Use of rose bengal iodine 131-tagged isotope for testing liver function in 4000 patients.", "content": "Within 12 years, 4000 patients with hepatic lesions, were examined by the author. Examinations included: clinical, laboratory, biopsy, together with radioactive Rose-bengal 131I. RBR test prooved to be more sensitive than known laboratory liver function tests. In diagnosis of chronic hepatitis and cirrhosis, histological examinations remain to be decisive, though biopsy is not always recommended from view point of prognosis. The isotope procedure has a great advantage besides to its sensitive registration of liver cells functions, it offers good informations about flowness of bile tract. The bladder diagrams increase by action of milk-chocolate meals (24 hours), after which, urine analysis, estimation of blood activity and resting-liver activity can be of value. Harm due to radiation in RBR test is a minimum, so it can be repeated several times in order to follow progression of liver diseases. RBR examination is recommended for all big hospitals.", "contents": "Use of rose bengal iodine 131-tagged isotope for testing liver function in 4000 patients. Within 12 years, 4000 patients with hepatic lesions, were examined by the author. Examinations included: clinical, laboratory, biopsy, together with radioactive Rose-bengal 131I. RBR test prooved to be more sensitive than known laboratory liver function tests. In diagnosis of chronic hepatitis and cirrhosis, histological examinations remain to be decisive, though biopsy is not always recommended from view point of prognosis. The isotope procedure has a great advantage besides to its sensitive registration of liver cells functions, it offers good informations about flowness of bile tract. The bladder diagrams increase by action of milk-chocolate meals (24 hours), after which, urine analysis, estimation of blood activity and resting-liver activity can be of value. Harm due to radiation in RBR test is a minimum, so it can be repeated several times in order to follow progression of liver diseases. RBR examination is recommended for all big hospitals.", "PMID": 970084}
{"id": "pubmed23n0032_7765", "title": "Endogenous motilin and lower esophageal sphincter pressure in man: clue to an association.", "content": "In man (n = 5), after intraduodenal instillation of 50 ml of 0.1 N hydrochloric acid the lower esophageal sphincter pressure (LESP) and plasma motilin concentrations rise concomitantly exceeding basal by about 80% (p less than 0.025) and 90% (p less than 0.05), respectively, at 3 to 4 minutes. These results suggest that the increase in LESP after duodenal acidification may be brought about by endogenously released motilin.", "contents": "Endogenous motilin and lower esophageal sphincter pressure in man: clue to an association. In man (n = 5), after intraduodenal instillation of 50 ml of 0.1 N hydrochloric acid the lower esophageal sphincter pressure (LESP) and plasma motilin concentrations rise concomitantly exceeding basal by about 80% (p less than 0.025) and 90% (p less than 0.05), respectively, at 3 to 4 minutes. These results suggest that the increase in LESP after duodenal acidification may be brought about by endogenously released motilin.", "PMID": 970085}
{"id": "pubmed23n0032_7766", "title": "Studies on the structure and function of Gomori-positive glial cells in the rat hypothalamus.", "content": "Electron-microscopic, histochemical and endocrinologic study of aldehyde-fuchsin-positive (Gomori-positive; GP) grains of rat brain periventricular glia (GP glia) was carried out. GP structures appear as a population of osmiophilic particles, which is heterogeneous in both shape and size. Laminar structures interspersed with fine granular material were seen in the GP granules. No activity of the lysosomal and mitochondrial enzymes could be observed. The reaction for peroxidase was also negative. The GP material was stained with PAS and Ziehl-Nielsen. There are apparently no lipoid inclusions in the GP grains. The primary red-orange fluorescence distinguishes the GP glia from other structures in the rat brain. So GP grains are a specific cytoplasmic formation having some similarity to lipofuscin. There was a considerable decrease in GP grains after administration of estradiol in ovariectomized rats and also in pregnant rats. Dopamine administration and starvation caused some reduction in GP grains. In the rat hypothalamus, distribution of the main mass of GP glia corresponds with the so-called hypophysiotropic area. The possible participation of GP glia in the neuroendocrinological process is discussed.", "contents": "Studies on the structure and function of Gomori-positive glial cells in the rat hypothalamus. Electron-microscopic, histochemical and endocrinologic study of aldehyde-fuchsin-positive (Gomori-positive; GP) grains of rat brain periventricular glia (GP glia) was carried out. GP structures appear as a population of osmiophilic particles, which is heterogeneous in both shape and size. Laminar structures interspersed with fine granular material were seen in the GP granules. No activity of the lysosomal and mitochondrial enzymes could be observed. The reaction for peroxidase was also negative. The GP material was stained with PAS and Ziehl-Nielsen. There are apparently no lipoid inclusions in the GP grains. The primary red-orange fluorescence distinguishes the GP glia from other structures in the rat brain. So GP grains are a specific cytoplasmic formation having some similarity to lipofuscin. There was a considerable decrease in GP grains after administration of estradiol in ovariectomized rats and also in pregnant rats. Dopamine administration and starvation caused some reduction in GP grains. In the rat hypothalamus, distribution of the main mass of GP glia corresponds with the so-called hypophysiotropic area. The possible participation of GP glia in the neuroendocrinological process is discussed.", "PMID": 970091}
{"id": "pubmed23n0032_7767", "title": "Cytological identification of sex in pig embryos at indifferent gonadal stages.", "content": "A reliable method of sex identification in pig embryos at sexually indifferent and early differentiated gonadal stages (age 21-26 days) was developed in association with ultrastructural studies on gonadal development and differentiation. The routine carbolfuchsin staining for the sex chromatin and the quinacrine mustard dihydrochloride staining for the fluorescent Y chromosome were not successful when applied to interphase cells. Therefore, it was necessary to develop a method for complete chromosome preparations. Head, heart and liver proved to have a sufficiently high spontaneous mitotic rate without any mitogenic stimulation. The tissues were suspended in the culture medium. Optimal conditions for colchicine treatment and hypotonization were tested and the details of the procedure are reported. This direct method yielded adequate metaphase plates for chromosome analyses in pig embryos. Because no culturing is used, the method is rapid and artefactual changes in the chromosomes during stimulated cell divisions in vitro are avoided.", "contents": "Cytological identification of sex in pig embryos at indifferent gonadal stages. A reliable method of sex identification in pig embryos at sexually indifferent and early differentiated gonadal stages (age 21-26 days) was developed in association with ultrastructural studies on gonadal development and differentiation. The routine carbolfuchsin staining for the sex chromatin and the quinacrine mustard dihydrochloride staining for the fluorescent Y chromosome were not successful when applied to interphase cells. Therefore, it was necessary to develop a method for complete chromosome preparations. Head, heart and liver proved to have a sufficiently high spontaneous mitotic rate without any mitogenic stimulation. The tissues were suspended in the culture medium. Optimal conditions for colchicine treatment and hypotonization were tested and the details of the procedure are reported. This direct method yielded adequate metaphase plates for chromosome analyses in pig embryos. Because no culturing is used, the method is rapid and artefactual changes in the chromosomes during stimulated cell divisions in vitro are avoided.", "PMID": 970092}
{"id": "pubmed23n0032_7768", "title": "Sexual dimorphism in the basilar part of the occipital bone of the dog (Canis familiaris).", "content": "Sexual dimorphism in the surface markings of the basilar part of the occipital bone of 34 male and 46 female dog's skulls were studied with the following results: 1 of the 34 male skulls showed a female type surface marking, 4 of the 46 female skulls showed surface markings of the opposite sex, while 2 of the male and 3 of the female skulls could not be classified as belonging to either type, so that 10 out of the 80 skulls could not be identified correctly, indicating that 87.5% of the skulls could be identified positively. Considering that there are hardly any sexual differences in the other surface markings or in the dentition of the dog's skull, these findings may be of some help in sexing the skull of the dog.", "contents": "Sexual dimorphism in the basilar part of the occipital bone of the dog (Canis familiaris). Sexual dimorphism in the surface markings of the basilar part of the occipital bone of 34 male and 46 female dog's skulls were studied with the following results: 1 of the 34 male skulls showed a female type surface marking, 4 of the 46 female skulls showed surface markings of the opposite sex, while 2 of the male and 3 of the female skulls could not be classified as belonging to either type, so that 10 out of the 80 skulls could not be identified correctly, indicating that 87.5% of the skulls could be identified positively. Considering that there are hardly any sexual differences in the other surface markings or in the dentition of the dog's skull, these findings may be of some help in sexing the skull of the dog.", "PMID": 970093}
{"id": "pubmed23n0032_7769", "title": "Non-specific esterases in the gustatory epithelia of man and dog.", "content": "(1) Simple esterase activity has been demonstrated in the gustatory epithelium of man and dog by the simultaneous coupling azo dye technique using alpha-naphthol and naphthol As acetate. Unfixed cryostat and fixed paraffin sections were used. (2) A peculiar pattern of simple esterase activity was encountered in which--contrary to what was to be expected--the taste bud-carrying papillae showed a very poor reaction while there was a gradual increase in the enzyme intensity as the epithelium was traced away from these papillae. (3) It seems that among the reported differences between simple esterases and cholinesterases is this differential activity in relation to the gemmal system. (4) A peculiar difference in the enzyme activity was reported between the unfixed cryostat and the fixed paraffin sections in the human material.", "contents": "Non-specific esterases in the gustatory epithelia of man and dog. (1) Simple esterase activity has been demonstrated in the gustatory epithelium of man and dog by the simultaneous coupling azo dye technique using alpha-naphthol and naphthol As acetate. Unfixed cryostat and fixed paraffin sections were used. (2) A peculiar pattern of simple esterase activity was encountered in which--contrary to what was to be expected--the taste bud-carrying papillae showed a very poor reaction while there was a gradual increase in the enzyme intensity as the epithelium was traced away from these papillae. (3) It seems that among the reported differences between simple esterases and cholinesterases is this differential activity in relation to the gemmal system. (4) A peculiar difference in the enzyme activity was reported between the unfixed cryostat and the fixed paraffin sections in the human material.", "PMID": 970094}
{"id": "pubmed23n0032_7770", "title": "Re-evaluation of the colic irrigation from the superior mesenteric artery.", "content": "156 abdominal preparations were explored by arteriography, corrosion and dissection. The ileocolic artery is the most constant collateral of the superior mesenteric artery. It provides a ramus colicus which is often confused with a true arteria colica dextra. The a. colica dextra, arising on the superior mesenteric artery, is found in only 13% of the abdominal preparations. The PNA 'a. colica media' covers a system of individually varying vessels: a. colica media, common stem of r. anguli coli and r. coli transversi; a. anguli coli, a. coli transversi; a. colica sinistra accessoria and a. coli transversi accessoria. Both mesenteric arteries may be linked by a intermesenteric arch (12%).", "contents": "Re-evaluation of the colic irrigation from the superior mesenteric artery. 156 abdominal preparations were explored by arteriography, corrosion and dissection. The ileocolic artery is the most constant collateral of the superior mesenteric artery. It provides a ramus colicus which is often confused with a true arteria colica dextra. The a. colica dextra, arising on the superior mesenteric artery, is found in only 13% of the abdominal preparations. The PNA 'a. colica media' covers a system of individually varying vessels: a. colica media, common stem of r. anguli coli and r. coli transversi; a. anguli coli, a. coli transversi; a. colica sinistra accessoria and a. coli transversi accessoria. Both mesenteric arteries may be linked by a intermesenteric arch (12%).", "PMID": 970095}
{"id": "pubmed23n0032_7771", "title": "Termination in the stellate ganglion of axons arising from the hilar vegetative plexus of the lung. Peripheral reflex arcs.", "content": "In cats the right lung was removed by thoracotomy. After 24-48 h the right stellate ganglion, the communicating branches between the right stellate ganglion and the right vagus, as well as the portion of both vagus nerves below and above the communicating branches, were processed for electron-microscopic investigation. Degeneration of synaptic axon terminals in the anteromedial part of the stellate ganglia, occurring after removal of the ipsilateral lung, shows that processes of ganglion cells located in the terminal ganglia of the bronchial tree establish synapses in the stellate ganglia. These results speak in favour of the existence of peripheral reflex arcs in the autonomic nervous system. For the time being, the data are not sufficient to define a new pathway related to the autonomic ganglia.", "contents": "Termination in the stellate ganglion of axons arising from the hilar vegetative plexus of the lung. Peripheral reflex arcs. In cats the right lung was removed by thoracotomy. After 24-48 h the right stellate ganglion, the communicating branches between the right stellate ganglion and the right vagus, as well as the portion of both vagus nerves below and above the communicating branches, were processed for electron-microscopic investigation. Degeneration of synaptic axon terminals in the anteromedial part of the stellate ganglia, occurring after removal of the ipsilateral lung, shows that processes of ganglion cells located in the terminal ganglia of the bronchial tree establish synapses in the stellate ganglia. These results speak in favour of the existence of peripheral reflex arcs in the autonomic nervous system. For the time being, the data are not sufficient to define a new pathway related to the autonomic ganglia.", "PMID": 970096}
{"id": "pubmed23n0032_7772", "title": "The diffuse hypertrophy of the lower esophagus and the focal hypertrophy of the pyloric musculature in adults.", "content": "The diffuse hypertrophy of the lower esophagus is a hypertrophy and hyperplasia of the smooth muscle fibres of the M. propria, together with a distortion of the muscular architecture. The esophagogram reveals an impaired peristalsis and expansile ability of the esophagus lumen. The focal hypertrophy of the pyloric musculature in adults is a locally circumscribed hypertrophy and hyperplasia of the M. propria. The concentric pattern of the M. propria is no longer maintained. Radiologically one recognizes a benign pylorus stenosis with a complete systole and an impaired diastolic dilatation. On the basis of these macroscopical, microscopical and radiological findings both lesions differ from the circular pyloric or esophageal stenosis which occurs at the same anatomical sites. We therefore feel, that both lesions are different entities.", "contents": "The diffuse hypertrophy of the lower esophagus and the focal hypertrophy of the pyloric musculature in adults. The diffuse hypertrophy of the lower esophagus is a hypertrophy and hyperplasia of the smooth muscle fibres of the M. propria, together with a distortion of the muscular architecture. The esophagogram reveals an impaired peristalsis and expansile ability of the esophagus lumen. The focal hypertrophy of the pyloric musculature in adults is a locally circumscribed hypertrophy and hyperplasia of the M. propria. The concentric pattern of the M. propria is no longer maintained. Radiologically one recognizes a benign pylorus stenosis with a complete systole and an impaired diastolic dilatation. On the basis of these macroscopical, microscopical and radiological findings both lesions differ from the circular pyloric or esophageal stenosis which occurs at the same anatomical sites. We therefore feel, that both lesions are different entities.", "PMID": 970086}
{"id": "pubmed23n0032_7773", "title": "An ultrastructural study of the relationship between pericytes and cerebral macrophages.", "content": "We studied, with the electron microscope, the normal cerebral cortex of white rats and zones adjacent to puncture wounds. The data obtained in this study, demonstrated the passage of pericytes to the nervous tissue, after the rupture of the basal membrane, and their transformation into macrophages. We suggest the possibility of the transformation of the pericytes into microglial cells.", "contents": "An ultrastructural study of the relationship between pericytes and cerebral macrophages. We studied, with the electron microscope, the normal cerebral cortex of white rats and zones adjacent to puncture wounds. The data obtained in this study, demonstrated the passage of pericytes to the nervous tissue, after the rupture of the basal membrane, and their transformation into macrophages. We suggest the possibility of the transformation of the pericytes into microglial cells.", "PMID": 970097}
{"id": "pubmed23n0032_7774", "title": "Arterial supply to the hypogastric and presacral nerves.", "content": "The hypogastric nerves take an arterial supply from the internal iliac artery either directly or from one of its main branches close to its origin from the parent trunk. Sometimes the supply is from the lower end of the common iliac artery. In half the cases examined the presacral nerve takes an arterial supply from the aorta at its bifurcation.", "contents": "Arterial supply to the hypogastric and presacral nerves. The hypogastric nerves take an arterial supply from the internal iliac artery either directly or from one of its main branches close to its origin from the parent trunk. Sometimes the supply is from the lower end of the common iliac artery. In half the cases examined the presacral nerve takes an arterial supply from the aorta at its bifurcation.", "PMID": 970098}
{"id": "pubmed23n0032_7775", "title": "Ultrastructure of articular cartilage of achondroplastic mice.", "content": "Articular chondrocytes of achondroplastic mice (cn/cn) resemble ultrastructurally those of their non-achondroplastic siblings (Cn/Cn or Cn/cn), except for premature deposition of glycogen and a tendency to undergo regression. The latter may be slight or extreme. The ultimate cause of the vulnerability of the chondrocytes and the cause of the heterogeneity of the reaction could not be determined with the method employed. Nevertheless, increased vulnerability accounts for cell death under conditions which are usually not injurious, and which may be either physiologic or altered by endogenous or exogenous factors.", "contents": "Ultrastructure of articular cartilage of achondroplastic mice. Articular chondrocytes of achondroplastic mice (cn/cn) resemble ultrastructurally those of their non-achondroplastic siblings (Cn/Cn or Cn/cn), except for premature deposition of glycogen and a tendency to undergo regression. The latter may be slight or extreme. The ultimate cause of the vulnerability of the chondrocytes and the cause of the heterogeneity of the reaction could not be determined with the method employed. Nevertheless, increased vulnerability accounts for cell death under conditions which are usually not injurious, and which may be either physiologic or altered by endogenous or exogenous factors.", "PMID": 970100}
{"id": "pubmed23n0032_7776", "title": "Effect of triamcinolone on the ultrastructure of mouse hepatocytes.", "content": "Premature female mice were injected daily with 1.5 mg/kg body weight of triamcinolone diacetate for 10 and 21 consecutive days. Electron-microscopic study of hepatocytes indicated the formation of 'giant' mitochondria concomitant with a marked increase in the number and size of lipid droplets. Hypertrophied mitochondria and enlarged lipid droplets were found to be in a close topographical association. The mechanism of mitochondrial enlargement is discussed as well as the latters' role in the hepatocytes' lipid metabolism.", "contents": "Effect of triamcinolone on the ultrastructure of mouse hepatocytes. Premature female mice were injected daily with 1.5 mg/kg body weight of triamcinolone diacetate for 10 and 21 consecutive days. Electron-microscopic study of hepatocytes indicated the formation of 'giant' mitochondria concomitant with a marked increase in the number and size of lipid droplets. Hypertrophied mitochondria and enlarged lipid droplets were found to be in a close topographical association. The mechanism of mitochondrial enlargement is discussed as well as the latters' role in the hepatocytes' lipid metabolism.", "PMID": 970101}
{"id": "pubmed23n0032_7777", "title": "Study in the changes of the proportions and numbers of the various glial cell types in the spinal cord of neonatal and young adult rats.", "content": "Glioblasts, astrocytes, microglia and the three classes of oligodendroyctes were enumerated in the grey and white matter of the spinal cord of rats of various ages. Both regions showed fairly similar results. Glioblasts, which made up the major glial population in the newborn rats, declined steadily with age and their number became negligible by 22 days post natum. They were absent in the young adult rats (aged 70 days). Contrary to this, the major glial types increased rapidly with age, the increase being most drastic in the oligodendrocytic population. The growth continued through about 22 days after birth and became more or less stabilized thereafter. Of the three classes of oligodendrocytes, the light cells appeared to develop first, followed by the medium dense cells and subsequently the dark ones. While there was a gradual disappearance of the light and medium dense cells with age, there was an accumulation of the dark cells, so that they were predominant in the spinal cord of the young adult rats.", "contents": "Study in the changes of the proportions and numbers of the various glial cell types in the spinal cord of neonatal and young adult rats. Glioblasts, astrocytes, microglia and the three classes of oligodendroyctes were enumerated in the grey and white matter of the spinal cord of rats of various ages. Both regions showed fairly similar results. Glioblasts, which made up the major glial population in the newborn rats, declined steadily with age and their number became negligible by 22 days post natum. They were absent in the young adult rats (aged 70 days). Contrary to this, the major glial types increased rapidly with age, the increase being most drastic in the oligodendrocytic population. The growth continued through about 22 days after birth and became more or less stabilized thereafter. Of the three classes of oligodendrocytes, the light cells appeared to develop first, followed by the medium dense cells and subsequently the dark ones. While there was a gradual disappearance of the light and medium dense cells with age, there was an accumulation of the dark cells, so that they were predominant in the spinal cord of the young adult rats.", "PMID": 970102}
{"id": "pubmed23n0032_7778", "title": "Fine structure of precapillary arterioles of skeletal muscle in the rat.", "content": "The submicroscopic structure of the precapillary arterioles with a diameter of 25-6 mum was followed in m. spinotrapezius of the rat. The walls of the precapillary arterioles of the diameter of 25-20 mum consist of the endothelium, one layer of smooth muscle cells, 3-4 bundles of nerve fibres and a one-layer coat of fibrocytes. Arterioles of the diameter of 20-10 mum have an incomplete fibrocytar coat. Arterioles of the diameter of 10-6 mum also have an incomplete, disseminated muscular layer. The side-branching of true capillaries was not detected. Hence the described arteriolar portion cannot be considered as a metarteriole, but as a regulation device of a segmentary type, regulating the blood flow through the corresponding part of the capillary network as a whole.", "contents": "Fine structure of precapillary arterioles of skeletal muscle in the rat. The submicroscopic structure of the precapillary arterioles with a diameter of 25-6 mum was followed in m. spinotrapezius of the rat. The walls of the precapillary arterioles of the diameter of 25-20 mum consist of the endothelium, one layer of smooth muscle cells, 3-4 bundles of nerve fibres and a one-layer coat of fibrocytes. Arterioles of the diameter of 20-10 mum have an incomplete fibrocytar coat. Arterioles of the diameter of 10-6 mum also have an incomplete, disseminated muscular layer. The side-branching of true capillaries was not detected. Hence the described arteriolar portion cannot be considered as a metarteriole, but as a regulation device of a segmentary type, regulating the blood flow through the corresponding part of the capillary network as a whole.", "PMID": 970103}
{"id": "pubmed23n0032_7779", "title": "Anatomical study of the cervical sympathetic trunk and ganglia in the albino rat (Mus norvegicus albinus).", "content": "Nine adult albino rats of both sexes were studied. 16 sympathetic trunks and ganglia were dissected in detail in eight rats. The right and left superior cervical ganglion and the sympathetic trunk below the ganglion were removed from an additional rat. The cell bodies of these ganglia and the axons of the trunks were counted with the aid of light and electron microscopy. Considering the number and location of ganglia and patterns of branching, the rat's cervical sympathetic nervous system compares closely with man's. There appears to be a relationship between body size and myelination of preganglionic neurons in the cervical sympathetic trunks, with smaller animals having the least number of myelinated fibers.", "contents": "Anatomical study of the cervical sympathetic trunk and ganglia in the albino rat (Mus norvegicus albinus). Nine adult albino rats of both sexes were studied. 16 sympathetic trunks and ganglia were dissected in detail in eight rats. The right and left superior cervical ganglion and the sympathetic trunk below the ganglion were removed from an additional rat. The cell bodies of these ganglia and the axons of the trunks were counted with the aid of light and electron microscopy. Considering the number and location of ganglia and patterns of branching, the rat's cervical sympathetic nervous system compares closely with man's. There appears to be a relationship between body size and myelination of preganglionic neurons in the cervical sympathetic trunks, with smaller animals having the least number of myelinated fibers.", "PMID": 970104}
{"id": "pubmed23n0032_7780", "title": "[Ultrastructure of the epithelium of the major pancreatic duct in sheep].", "content": "Light-and electron-microscopic and some histochemical investigations on the epithelium or the ductus pancreaticus major were carried out in sheep. At least six different cell types are recognizable: (1) nondifferentiated duct cells; (2) cells containing apical secretory granules; (3) goblet cells; the mucosubstances of type 2 and 3 are PAS- and Alcian-blue-positive, also reacting wih methenamine silver; (4) ciliated cells, containing a single cilium with the microtubular pattern 9+2; (5) tuft cells with extremely long and wide microvilli and a pear-shaped cell body; (6) migrating cells, mainly lymphocytes and some assumed eosinophils, showing reaction to Mg++-activated ATPase.", "contents": "[Ultrastructure of the epithelium of the major pancreatic duct in sheep]. Light-and electron-microscopic and some histochemical investigations on the epithelium or the ductus pancreaticus major were carried out in sheep. At least six different cell types are recognizable: (1) nondifferentiated duct cells; (2) cells containing apical secretory granules; (3) goblet cells; the mucosubstances of type 2 and 3 are PAS- and Alcian-blue-positive, also reacting wih methenamine silver; (4) ciliated cells, containing a single cilium with the microtubular pattern 9+2; (5) tuft cells with extremely long and wide microvilli and a pear-shaped cell body; (6) migrating cells, mainly lymphocytes and some assumed eosinophils, showing reaction to Mg++-activated ATPase.", "PMID": 970105}
{"id": "pubmed23n0032_7781", "title": "Preparation of fresh-frozen dried ultrathin sections by drying without vacuum.", "content": "Fresh-frozen dried ultrathin sections were prepared without fixation, embedding or staining. Small blocks of tissues were chilled by making contact with copper blocks precooled with liquid nitrogen according to a procedure modified from that of Christensen. Sections were cut at -95 degrees C with a Reichert Um 2 ultramicrotome fitted with cryoaccessories, using dry glass knives. The sections were dried for 15 min or more on formvar-coated grids by a flow of dry nitrogen gas at -95 degrees C in a newly devised glass chamber placed in the cryokit compartment. The sections showed only a few holes attributable to ice crystal formation. Holes were found in the intercellular spaces as well as within cells. The sections were observed without staining and gave enough contrast to permit recognition of organelles such as nucleus, perinuclear cisternae, nucleolus, interchromatin granules, perichromatinic granules and secretory granules in the pancreatic exocrine cells. In the basal part of the proximal convoluted tubules of mouse kidney, abundant mitochondria were seen. Mitochondrial cristae and lines corresponding to the basal infoldings of the renal convoluted tubules were less dense than their surroundings. This method can yield ultrathin sections lending themselves to investigations of the distribution of elements with electron-probe X-ray microanalyzer and of diffusible substances by dry-mount autoradiography at the electron-microscopic level.", "contents": "Preparation of fresh-frozen dried ultrathin sections by drying without vacuum. Fresh-frozen dried ultrathin sections were prepared without fixation, embedding or staining. Small blocks of tissues were chilled by making contact with copper blocks precooled with liquid nitrogen according to a procedure modified from that of Christensen. Sections were cut at -95 degrees C with a Reichert Um 2 ultramicrotome fitted with cryoaccessories, using dry glass knives. The sections were dried for 15 min or more on formvar-coated grids by a flow of dry nitrogen gas at -95 degrees C in a newly devised glass chamber placed in the cryokit compartment. The sections showed only a few holes attributable to ice crystal formation. Holes were found in the intercellular spaces as well as within cells. The sections were observed without staining and gave enough contrast to permit recognition of organelles such as nucleus, perinuclear cisternae, nucleolus, interchromatin granules, perichromatinic granules and secretory granules in the pancreatic exocrine cells. In the basal part of the proximal convoluted tubules of mouse kidney, abundant mitochondria were seen. Mitochondrial cristae and lines corresponding to the basal infoldings of the renal convoluted tubules were less dense than their surroundings. This method can yield ultrathin sections lending themselves to investigations of the distribution of elements with electron-probe X-ray microanalyzer and of diffusible substances by dry-mount autoradiography at the electron-microscopic level.", "PMID": 970106}
{"id": "pubmed23n0032_7782", "title": "[Wall structure of cisterna chyli in humans studied by light and scanning electron microscopy].", "content": "Comparing light and scanning electron microscopy of a chronically lymphostatic and two normal cisternae chyli made visible a normally folded and smoothened (under load) cisternal wall. Different orifices of the supplying lymph-collecting vessels with varying valve structures were seen. The functional meaning of the orifice types and of the valves for the uptake of lymph and chylus into the cisterna as well as for the blockage of lymphatic reflux out of the filled cisterna were discussed.", "contents": "[Wall structure of cisterna chyli in humans studied by light and scanning electron microscopy]. Comparing light and scanning electron microscopy of a chronically lymphostatic and two normal cisternae chyli made visible a normally folded and smoothened (under load) cisternal wall. Different orifices of the supplying lymph-collecting vessels with varying valve structures were seen. The functional meaning of the orifice types and of the valves for the uptake of lymph and chylus into the cisterna as well as for the blockage of lymphatic reflux out of the filled cisterna were discussed.", "PMID": 970107}
{"id": "pubmed23n0032_7783", "title": "Electromyographic study of the m. biceps brachii in movements at the glenohumeral joint.", "content": "Our electromyographic studies on the participation of the m. biceps brachii in the movements of the glenohumeral joint, performed in 20 young adult male volunteers, permit the following conclusions: (1) In the movement of arm flexion, performed against resistance and with extended elbow, both the long and the short heads of the m. biceps brachii were active in all 30 volunteers examined. (2) The same movement of the arm, arm flexion with extended elbow, now without resistance, that is to say performed freely, showed both the long and short heads of the m. biceps brachii active in the majority of cases. (3) In the movements of arm extension, performed freely and aganst resistance, with extended elbow, and also in the movement of lateral arm rotatin with flexed elbow, with and without resistance, the m. biceps brachii was absolutely inactive in all 30 volunteers examined. (4) In the movement of arm abduction, with extended elbow, the element of resistance to the performance showed itself to be important, since without it the m. biceps brachii was absolutely inactive; in the movement performed against resistance both heads of the muscle showed activity in 3 out of the 30 volunteers tested. (5) In the movements of arm abduction with and without resistance, with flexed elbow, the m. biceps brachii was active on occasion. (6) In the Movements of arm adduction against resistance, with the elbow either flexed or extended, it was only the short head of the m. biceps brachii which was active, and this in half of the cases examined. (7) In the movements of medical rotation of the arm, with extended elbow, performed freely or against resistance, the long and the short heads of the m. biceps brachii each showed a different behaviour: the long head was always inactive, whereas the short one was seldom active.", "contents": "Electromyographic study of the m. biceps brachii in movements at the glenohumeral joint. Our electromyographic studies on the participation of the m. biceps brachii in the movements of the glenohumeral joint, performed in 20 young adult male volunteers, permit the following conclusions: (1) In the movement of arm flexion, performed against resistance and with extended elbow, both the long and the short heads of the m. biceps brachii were active in all 30 volunteers examined. (2) The same movement of the arm, arm flexion with extended elbow, now without resistance, that is to say performed freely, showed both the long and short heads of the m. biceps brachii active in the majority of cases. (3) In the movements of arm extension, performed freely and aganst resistance, with extended elbow, and also in the movement of lateral arm rotatin with flexed elbow, with and without resistance, the m. biceps brachii was absolutely inactive in all 30 volunteers examined. (4) In the movement of arm abduction, with extended elbow, the element of resistance to the performance showed itself to be important, since without it the m. biceps brachii was absolutely inactive; in the movement performed against resistance both heads of the muscle showed activity in 3 out of the 30 volunteers tested. (5) In the movements of arm abduction with and without resistance, with flexed elbow, the m. biceps brachii was active on occasion. (6) In the Movements of arm adduction against resistance, with the elbow either flexed or extended, it was only the short head of the m. biceps brachii which was active, and this in half of the cases examined. (7) In the movements of medical rotation of the arm, with extended elbow, performed freely or against resistance, the long and the short heads of the m. biceps brachii each showed a different behaviour: the long head was always inactive, whereas the short one was seldom active.", "PMID": 970108}
{"id": "pubmed23n0032_7784", "title": "Eyedness.", "content": "The so-called 'eyedness' with regard to fixation of the gaze on a particular point has been investigated on 283 right-handed and 30 left-handed healthy persons of Central India. The results show that the majority (65.81%) of them are right-eyed, and that the eyedness is not influenced by sex or handedness. Though the left cerebral hemisphere seems to play a dominant role in gaze fixation in the greater proportion of the population, consideration of the joint of the two cerebral hemispheres for individual functions of the body has been advocated.", "contents": "Eyedness. The so-called 'eyedness' with regard to fixation of the gaze on a particular point has been investigated on 283 right-handed and 30 left-handed healthy persons of Central India. The results show that the majority (65.81%) of them are right-eyed, and that the eyedness is not influenced by sex or handedness. Though the left cerebral hemisphere seems to play a dominant role in gaze fixation in the greater proportion of the population, consideration of the joint of the two cerebral hemispheres for individual functions of the body has been advocated.", "PMID": 970109}
{"id": "pubmed23n0032_7785", "title": "Experimental cerebral ischemia in Mongolian gerbils. IV. Behaviour of biogenic amines.", "content": "Behaviour of biogenic amines was studied in the brains of Mongolian gerbils subjected to unilateral occlusion of the common carotid artery. Assays on the hemispheres ipsilateral to occlusion revealed in symptom-positive animals a progressive decrease in norepinephrine and dopamine, and an increase in serotonin throughout the duration of an ischemic insult. In post-ischemic periods following the release of the clip, changes in biogenic amine levels generally conformed to the principles of a previously described \"maturation\" phenomenon, with delayed reactions occurring after the shorter ischemic insults.", "contents": "Experimental cerebral ischemia in Mongolian gerbils. IV. Behaviour of biogenic amines. Behaviour of biogenic amines was studied in the brains of Mongolian gerbils subjected to unilateral occlusion of the common carotid artery. Assays on the hemispheres ipsilateral to occlusion revealed in symptom-positive animals a progressive decrease in norepinephrine and dopamine, and an increase in serotonin throughout the duration of an ischemic insult. In post-ischemic periods following the release of the clip, changes in biogenic amine levels generally conformed to the principles of a previously described \"maturation\" phenomenon, with delayed reactions occurring after the shorter ischemic insults.", "PMID": 970110}
{"id": "pubmed23n0032_7786", "title": "Status spongiosus of rat central nervous system induced by actinomycin D.", "content": "The effect on central myelin of Actinomycin D, an RNA--and, secondarily, a protein-synthesis inhibitor, has been studied by light and electron microscopy. The intracranial injection of this drug produced an extensive status spongiosus of the white matter in the cerebrum, cerebellum, brain stem and optic nerve within 48 h. The status spongiosus was due to vacuole formation within the myelin sheath and to enlargement of the extracellular space. Three types of vacuoles were observed: (a) the most common varieties formed between the inner tongue and the remainder of the myelin sheath; (b) a second variety formed by enlargement of the periaxonal space with separation of the axon from its myelin sheath, and (c) a less common type of vacuolization was due to splitting of the myelin lamellae at the interperiod line to form large intramyelinic vacuoles. Myelinic vacuoles were preceded by nuclear and cytoplasmic changes in oligodendrocytes, which included nucleolar segregation, disaggregation, and diminution in number of ribosomes. These changes were similar to those previously reported in a variety of cells exposed to Actinomycin D. It is suggested that myelin vacuoles result secondarily from the Actinomycin D inhibitory effect on oligodendroglial RNA--and protein-synthesis, rather than from a direct effect of this drug on the myelin sheath.", "contents": "Status spongiosus of rat central nervous system induced by actinomycin D. The effect on central myelin of Actinomycin D, an RNA--and, secondarily, a protein-synthesis inhibitor, has been studied by light and electron microscopy. The intracranial injection of this drug produced an extensive status spongiosus of the white matter in the cerebrum, cerebellum, brain stem and optic nerve within 48 h. The status spongiosus was due to vacuole formation within the myelin sheath and to enlargement of the extracellular space. Three types of vacuoles were observed: (a) the most common varieties formed between the inner tongue and the remainder of the myelin sheath; (b) a second variety formed by enlargement of the periaxonal space with separation of the axon from its myelin sheath, and (c) a less common type of vacuolization was due to splitting of the myelin lamellae at the interperiod line to form large intramyelinic vacuoles. Myelinic vacuoles were preceded by nuclear and cytoplasmic changes in oligodendrocytes, which included nucleolar segregation, disaggregation, and diminution in number of ribosomes. These changes were similar to those previously reported in a variety of cells exposed to Actinomycin D. It is suggested that myelin vacuoles result secondarily from the Actinomycin D inhibitory effect on oligodendroglial RNA--and protein-synthesis, rather than from a direct effect of this drug on the myelin sheath.", "PMID": 970111}
{"id": "pubmed23n0032_7787", "title": "The brain-specific S 100 protein in small cerebral stab wounds in the rat: a quantitative study.", "content": "A quantitative study of the changes in water-soluble proteins and water-soluble S 100 was made in stab-wounded rat frontal cortex as compared to unoperated controls. No great changes occurred until 30 days after the injury. At that time there was no change in the amount of water-soluble  S 100 protein/g wet weight, but a large decrease in the amount of water-soluble proteins/g wet weight and thus a proportionate increase in the amount of water-soluble S 100 protein/mg of water-soluble proteins. The significance of the results is discussed.", "contents": "The brain-specific S 100 protein in small cerebral stab wounds in the rat: a quantitative study. A quantitative study of the changes in water-soluble proteins and water-soluble S 100 was made in stab-wounded rat frontal cortex as compared to unoperated controls. No great changes occurred until 30 days after the injury. At that time there was no change in the amount of water-soluble  S 100 protein/g wet weight, but a large decrease in the amount of water-soluble proteins/g wet weight and thus a proportionate increase in the amount of water-soluble S 100 protein/mg of water-soluble proteins. The significance of the results is discussed.", "PMID": 970112}
{"id": "pubmed23n0032_7788", "title": "Cycloleucine uptake in the brainstem of thiamine-deficient rats.", "content": "Immature female rats were subjected to acute dietary deficiency of thiamine. An autoradiographic method was used in the semi-quantitative determination of concentration of 1-aminocyclopentane-1-carboxylic acid-carboxylic-14C (cycloleucine) in brainstem regions after intravenous administration of tracer quantities. The time course of tissue concentrations was followed and compared with that of normal and isocaloric control animals. Our data indicate that thiamine deficiency of sufficient magnitude to induce brainstem lesions has an effect on the transport of cycloleucine. The initial 2 min values in the nuclear areas are appreciably reduced whereas the 6 min values are significantly elevated as compared with controls; no change was demonstrated in the white matter of the inferior cerebellar peduncle. The effect on transport appears to be more on the mechanism of efflux than of influx in terms of blood brain barrier function.", "contents": "Cycloleucine uptake in the brainstem of thiamine-deficient rats. Immature female rats were subjected to acute dietary deficiency of thiamine. An autoradiographic method was used in the semi-quantitative determination of concentration of 1-aminocyclopentane-1-carboxylic acid-carboxylic-14C (cycloleucine) in brainstem regions after intravenous administration of tracer quantities. The time course of tissue concentrations was followed and compared with that of normal and isocaloric control animals. Our data indicate that thiamine deficiency of sufficient magnitude to induce brainstem lesions has an effect on the transport of cycloleucine. The initial 2 min values in the nuclear areas are appreciably reduced whereas the 6 min values are significantly elevated as compared with controls; no change was demonstrated in the white matter of the inferior cerebellar peduncle. The effect on transport appears to be more on the mechanism of efflux than of influx in terms of blood brain barrier function.", "PMID": 970113}
{"id": "pubmed23n0032_7789", "title": "[Neurosecretoric nerve-cell choristoma in the anterior pituitary (author's transl)].", "content": "Report on a blastomatous dysgenesia (choristoma) with dystopic accumulation of neurosecretoric nerve-cells in the anterior lobe of hypophysis of a 65 years old woman without clinical symptoms.", "contents": "[Neurosecretoric nerve-cell choristoma in the anterior pituitary (author's transl)]. Report on a blastomatous dysgenesia (choristoma) with dystopic accumulation of neurosecretoric nerve-cells in the anterior lobe of hypophysis of a 65 years old woman without clinical symptoms.", "PMID": 970114}
{"id": "pubmed23n0032_7790", "title": "Crush injury to peripheral nerve. An electron microscope study employing horseradish peroxidase.", "content": "Electron microscope observations were made of rat peroneal nerve after crushing using intravenously injected horseradish peroxidase (HRP) as a tracer protein to indicate changes in vascular permeability. At 1/2 h and 2 d after the crush there was gross leakage of HRP from damaged capillaries at the site of injury but none from vessels above or below this. Ultrastructurally vessels at the site of crush showed broken and separated endothelial cells. Proximally and distally there was little abnormal in the vessel walls; vesicles containing HRP were absent and tight-junctions between cells remained intact. Twenty-one days after the crush, leakage of HRP was found both at the site of crush and along the distal segment. The only change in vessel walls was an obvious increase in vesicles filled with HRP. Tracer was also found both in perivascular locations and throughout the endoneurial space.", "contents": "Crush injury to peripheral nerve. An electron microscope study employing horseradish peroxidase. Electron microscope observations were made of rat peroneal nerve after crushing using intravenously injected horseradish peroxidase (HRP) as a tracer protein to indicate changes in vascular permeability. At 1/2 h and 2 d after the crush there was gross leakage of HRP from damaged capillaries at the site of injury but none from vessels above or below this. Ultrastructurally vessels at the site of crush showed broken and separated endothelial cells. Proximally and distally there was little abnormal in the vessel walls; vesicles containing HRP were absent and tight-junctions between cells remained intact. Twenty-one days after the crush, leakage of HRP was found both at the site of crush and along the distal segment. The only change in vessel walls was an obvious increase in vesicles filled with HRP. Tracer was also found both in perivascular locations and throughout the endoneurial space.", "PMID": 970115}
{"id": "pubmed23n0032_7791", "title": "Studies of transitional cell tumours of the bladder. Prognosis and causes of death.", "content": "Transitional cell tumours of the bladder from a total of 228 patients were histologically classified as papillomas, papillomatous carcinomas and non-papillomatous carcinomas. Each group was subdivided into four grades of dysplasia. Papillomas and papillomatous carcinomas occurred in younger patients at a higher rate than non-papillomatous carcinomas. The 5-year-survival of patients with papillomas and carcinomas was 70 per cent and 26 per cent, respectively. Among patients with papillomas with dysplasia grades 1 and 2 the survival rate was almost identical. In the group of patients with papilloma thrombo-embolic diseases were the most common cause of death. Carcinoma of the bladder developed in about 30 per cent of the patients in this group. Recurrence of the papilloma only rarely changed the grade of dysplasia. If the recurrence was in the form of a carcinoma, an increase in the grade of dysplasia was common. The survival was more favourable among patients with carcinoma dysplasia grade 2 than among those with dysplasia grade 3. The rate of survival was higher in the group of patients with papillomatous carcinoma than among patients with non-papillomatous carcinomas. Among the decreased patients with primary carcinomas, 77 per cent died with carcinoma of the bladder. To a certain degree, the grade of tumour cell dysplasia seems to be an expression of the malignancy of the tumours. The duration of the disease and the appearance of tumours (papillomatous, non-papillomatous) may have relation to the patients' \"defence\" system. At autopsy, the papillomatous and non-papillomatous carcinomas were found to be similarly disseminated regardless of the difference in survival rate.", "contents": "Studies of transitional cell tumours of the bladder. Prognosis and causes of death. Transitional cell tumours of the bladder from a total of 228 patients were histologically classified as papillomas, papillomatous carcinomas and non-papillomatous carcinomas. Each group was subdivided into four grades of dysplasia. Papillomas and papillomatous carcinomas occurred in younger patients at a higher rate than non-papillomatous carcinomas. The 5-year-survival of patients with papillomas and carcinomas was 70 per cent and 26 per cent, respectively. Among patients with papillomas with dysplasia grades 1 and 2 the survival rate was almost identical. In the group of patients with papilloma thrombo-embolic diseases were the most common cause of death. Carcinoma of the bladder developed in about 30 per cent of the patients in this group. Recurrence of the papilloma only rarely changed the grade of dysplasia. If the recurrence was in the form of a carcinoma, an increase in the grade of dysplasia was common. The survival was more favourable among patients with carcinoma dysplasia grade 2 than among those with dysplasia grade 3. The rate of survival was higher in the group of patients with papillomatous carcinoma than among patients with non-papillomatous carcinomas. Among the decreased patients with primary carcinomas, 77 per cent died with carcinoma of the bladder. To a certain degree, the grade of tumour cell dysplasia seems to be an expression of the malignancy of the tumours. The duration of the disease and the appearance of tumours (papillomatous, non-papillomatous) may have relation to the patients' \"defence\" system. At autopsy, the papillomatous and non-papillomatous carcinomas were found to be similarly disseminated regardless of the difference in survival rate.", "PMID": 970124}
{"id": "pubmed23n0032_7792", "title": "Urothelial changes of the renal papillae in Sprague-Dawley rats induced by long term feeding of phenacetin.", "content": "Thirty female Sprague-Dawley rats were fed 0.535 per cent phenacetin in the diet for up to 110 weeks. Twenty-six of these rats developed urothelial hyperplasia, partly papillary, of the renal papillae. Twenty-eight rats showed dilatation of the vasa recta frequently associated with thrombus formation and calcification. One phenacetin fed rat had epithelial hyperplasia associated with chronic pyelitis. In 2 of the 30 control rats urothelial hyperplasia was found to be associated with chronic pyelitis. The hyperplastic urothelial changes and vascular changes were often, but not always, present simultaneously. One control rat developed a mammary carcinoma, as compared with 5 rats in the phenacetin group. Four phenacetin fed rats developed carcinoma of the ear duct. The results of the present investigation provide evidence that phenacetin can induce proliferative lesions of the urothelium of the rat renal pelvis with weak carcinogenic activity in the ear duct and mammary glands.", "contents": "Urothelial changes of the renal papillae in Sprague-Dawley rats induced by long term feeding of phenacetin. Thirty female Sprague-Dawley rats were fed 0.535 per cent phenacetin in the diet for up to 110 weeks. Twenty-six of these rats developed urothelial hyperplasia, partly papillary, of the renal papillae. Twenty-eight rats showed dilatation of the vasa recta frequently associated with thrombus formation and calcification. One phenacetin fed rat had epithelial hyperplasia associated with chronic pyelitis. In 2 of the 30 control rats urothelial hyperplasia was found to be associated with chronic pyelitis. The hyperplastic urothelial changes and vascular changes were often, but not always, present simultaneously. One control rat developed a mammary carcinoma, as compared with 5 rats in the phenacetin group. Four phenacetin fed rats developed carcinoma of the ear duct. The results of the present investigation provide evidence that phenacetin can induce proliferative lesions of the urothelium of the rat renal pelvis with weak carcinogenic activity in the ear duct and mammary glands.", "PMID": 970125}
{"id": "pubmed23n0032_7793", "title": "A synergistic effect of oestradiol and prolactin influencing the incidence of 3-methylcholanthrene induced cerivical carcinomas in mice.", "content": "Castrated NMRI mice were laparotomized and a thread impregnated with beeswax-methylcholanthrene was inserted into the uterine cervix. Beginning on the day of operation and for a further 5 days the animals were injected with oestradiol, prolactin, oestradiol-prolactin, oestradiol-prolactin-progesterone, or the solvents for the hormones only. One group of animals were injected with oestradiol-prolactin for 6 days and later with progesterone every third day until death. The animals were killed one or 4 weeks after the operation. Among the one-week animals the number of cervices presenting epithelial downgrowths (\"buds\") into the stroma was higher after treatment with a combination of oestradiol and prolactin than after treatment with each hormone separately or among the controls. Four weeks after operation, the incidence of squamous cervical carcinomas was seen to be significantly higher among animals injected with both oestradiol and prolactin than in controls or in those injected with oestradiol or prolactin alone. Progesterone had no definite effect on the oestradiol-prolactin induced incidence. The mechanism behind the synergistic effect of prolactin and oestradiol is discussed.", "contents": "A synergistic effect of oestradiol and prolactin influencing the incidence of 3-methylcholanthrene induced cerivical carcinomas in mice. Castrated NMRI mice were laparotomized and a thread impregnated with beeswax-methylcholanthrene was inserted into the uterine cervix. Beginning on the day of operation and for a further 5 days the animals were injected with oestradiol, prolactin, oestradiol-prolactin, oestradiol-prolactin-progesterone, or the solvents for the hormones only. One group of animals were injected with oestradiol-prolactin for 6 days and later with progesterone every third day until death. The animals were killed one or 4 weeks after the operation. Among the one-week animals the number of cervices presenting epithelial downgrowths (\"buds\") into the stroma was higher after treatment with a combination of oestradiol and prolactin than after treatment with each hormone separately or among the controls. Four weeks after operation, the incidence of squamous cervical carcinomas was seen to be significantly higher among animals injected with both oestradiol and prolactin than in controls or in those injected with oestradiol or prolactin alone. Progesterone had no definite effect on the oestradiol-prolactin induced incidence. The mechanism behind the synergistic effect of prolactin and oestradiol is discussed.", "PMID": 970126}
{"id": "pubmed23n0032_7794", "title": "Participation of renal, but not of submaxillary renin in the homeostasis of the blood pressure after experimentally induced hypotension in mice.", "content": "While hypotension elicited a marked increase in plasma renin concentration in conscious normal mice, no increase was provoked in previously nephrectomized mice in spite of the high renin content of their submaxillary glands. The role of the increased release of renal renin for the homeostasis of the blood pressure was shown by the decrease in pressure which followed blockade of the renin system. Contrary to Saralasin which did not change the blood pressure in nephrectomized mice, injections of SQ 20.881 did in some mice result in a decrease in blood pressure, which was probably caused by its ability to inhibit bradykininases. Both Saralasin and SQ 20.881 elicited marked increases in plasma renin in normal, but not in nephrectomized mice, showing that, while renal renin release is controlled by the plasma angiotensin II concentration, this does not apply to submaxillary renin release.", "contents": "Participation of renal, but not of submaxillary renin in the homeostasis of the blood pressure after experimentally induced hypotension in mice. While hypotension elicited a marked increase in plasma renin concentration in conscious normal mice, no increase was provoked in previously nephrectomized mice in spite of the high renin content of their submaxillary glands. The role of the increased release of renal renin for the homeostasis of the blood pressure was shown by the decrease in pressure which followed blockade of the renin system. Contrary to Saralasin which did not change the blood pressure in nephrectomized mice, injections of SQ 20.881 did in some mice result in a decrease in blood pressure, which was probably caused by its ability to inhibit bradykininases. Both Saralasin and SQ 20.881 elicited marked increases in plasma renin in normal, but not in nephrectomized mice, showing that, while renal renin release is controlled by the plasma angiotensin II concentration, this does not apply to submaxillary renin release.", "PMID": 970127}
{"id": "pubmed23n0032_7795", "title": "Polyvinylpyrrolidone-storage disease. Light microscopical, ultrastructural and chemical verification.", "content": "The light and electron microscopical findings in the polyvinylpyrrolidine-storage disease are reported on the basis of biopsies of skin, striated muscle, bone marrow and liver from one patient and a subcutaneous nodule from another patient. Both patients suffer from diabetes insipidus and have been treated for several years with Insipidin retard, which contains polyvinylpyrrolidone (PVP) as the retarding agent. Deposits of PVP have been demonstrated in all the tissues examined and can easily be recognized by certain staining qualities. The combination of haematoxylin-eosin, elastin (Weigert), alkaline Congo red and Sirius red for amyloid and PTAH is specially to be recommended. The ultrastructural findings consist of intracellular vacuoles containing a granular material and probably representing lysosomes. The final identification of the nature of the deposits has been made by spectrophotometric analysis. The suspicion of a case of PVP-storage disease should result in a skin biopsy which usually is sufficient for the diagnosis.", "contents": "Polyvinylpyrrolidone-storage disease. Light microscopical, ultrastructural and chemical verification. The light and electron microscopical findings in the polyvinylpyrrolidine-storage disease are reported on the basis of biopsies of skin, striated muscle, bone marrow and liver from one patient and a subcutaneous nodule from another patient. Both patients suffer from diabetes insipidus and have been treated for several years with Insipidin retard, which contains polyvinylpyrrolidone (PVP) as the retarding agent. Deposits of PVP have been demonstrated in all the tissues examined and can easily be recognized by certain staining qualities. The combination of haematoxylin-eosin, elastin (Weigert), alkaline Congo red and Sirius red for amyloid and PTAH is specially to be recommended. The ultrastructural findings consist of intracellular vacuoles containing a granular material and probably representing lysosomes. The final identification of the nature of the deposits has been made by spectrophotometric analysis. The suspicion of a case of PVP-storage disease should result in a skin biopsy which usually is sufficient for the diagnosis.", "PMID": 970128}
{"id": "pubmed23n0032_7796", "title": "Diabetic cardiopathy. Quantitative histological studies of the heart from young juvenile diabetics.", "content": "A quantitative morphological study, at light microscopical level, of hearts from young diabetics and nondiabetics was performed. The groups were comparable with respect to sex-ratio, age, heart weight and blood pressure. Findings in the hearts were as follows: In arterioles in which the luminal diameter ranged between 15-50 mu, a strongly PAS-positive stained vessel wall occurred at a higher frequency among diabetics than among non-diabetics (75 per cent respectively 33 per cent). The PAS-positive structures, however, occupied the same area of the vessel wall in the two groups. In arterioles from diabetics, the number of cells in tunica media was increased as compared with that in non-diabetics (2 p less than 0.01). The amount of perivascular connective tissue was also increased in the diabetics (2 p less than 0.01). There was no indication of an endothelial cell proliferation in the PAS-positive stained vessels from diabetics and non-diabetics. The wall of the capillaries was not thickened and it was not more PAS-positive in the hearts from diabetics than in those from non-diabetics. Moreover, the number of capillaries per square millimeter of heart muscle was the same in the two groups of hearts. In the present light microscopical study, the diabetic micro-angiography of the heart was demonstrable in the arterioles. The capillaropathy known to occur in other organs was not present in the heart muscle from patients with diabetes of long standing.", "contents": "Diabetic cardiopathy. Quantitative histological studies of the heart from young juvenile diabetics. A quantitative morphological study, at light microscopical level, of hearts from young diabetics and nondiabetics was performed. The groups were comparable with respect to sex-ratio, age, heart weight and blood pressure. Findings in the hearts were as follows: In arterioles in which the luminal diameter ranged between 15-50 mu, a strongly PAS-positive stained vessel wall occurred at a higher frequency among diabetics than among non-diabetics (75 per cent respectively 33 per cent). The PAS-positive structures, however, occupied the same area of the vessel wall in the two groups. In arterioles from diabetics, the number of cells in tunica media was increased as compared with that in non-diabetics (2 p less than 0.01). The amount of perivascular connective tissue was also increased in the diabetics (2 p less than 0.01). There was no indication of an endothelial cell proliferation in the PAS-positive stained vessels from diabetics and non-diabetics. The wall of the capillaries was not thickened and it was not more PAS-positive in the hearts from diabetics than in those from non-diabetics. Moreover, the number of capillaries per square millimeter of heart muscle was the same in the two groups of hearts. In the present light microscopical study, the diabetic micro-angiography of the heart was demonstrable in the arterioles. The capillaropathy known to occur in other organs was not present in the heart muscle from patients with diabetes of long standing.", "PMID": 970129}
{"id": "pubmed23n0032_7797", "title": "Experimental porcine nephropathy: changes of renal function and structure perorally induced by crystalline ochratoxin A.", "content": "Nine pigs were fed crystalline ochratoxin A in amounts corresponding to a feed level of 1 mg per kg for 3 months. The only observable lesion developed was a kidney damage, identical to the naturally occurring porcine nephropathy. The changes of renal function was characterized by impairment of proximal tubular function, indicated by a decrease of the ratio TmPAH/CIn, of the ability to concentrate urine, and by an increased urinary excretion of glucose. The decrease of the ratio TmPAH/CIn is correlated with time of exposure to ochratoxin A. The changes of renal structure were characterized by degeneration of the proximal tubules, leading to tubular atrophy accompanied by interstitial fibrosis. At the end of the experiment the kidney, liver, adipose and muscular tissue of the slaughtered pigs contained sizable amounts of ochratoxin A residues. As the pigs would have passed the meat inspection this represents a possible health problem. The changes observed in this study are identical to those observed by feeding to pigs grains naturally contaminated with ochratoxin A.", "contents": "Experimental porcine nephropathy: changes of renal function and structure perorally induced by crystalline ochratoxin A. Nine pigs were fed crystalline ochratoxin A in amounts corresponding to a feed level of 1 mg per kg for 3 months. The only observable lesion developed was a kidney damage, identical to the naturally occurring porcine nephropathy. The changes of renal function was characterized by impairment of proximal tubular function, indicated by a decrease of the ratio TmPAH/CIn, of the ability to concentrate urine, and by an increased urinary excretion of glucose. The decrease of the ratio TmPAH/CIn is correlated with time of exposure to ochratoxin A. The changes of renal structure were characterized by degeneration of the proximal tubules, leading to tubular atrophy accompanied by interstitial fibrosis. At the end of the experiment the kidney, liver, adipose and muscular tissue of the slaughtered pigs contained sizable amounts of ochratoxin A residues. As the pigs would have passed the meat inspection this represents a possible health problem. The changes observed in this study are identical to those observed by feeding to pigs grains naturally contaminated with ochratoxin A.", "PMID": 970130}
{"id": "pubmed23n0032_7798", "title": "Routine procedure for determining goblet cell density in the mucosa of the respiratory tract.", "content": "In two normal nasal septa, fine-dissected and stained by PAS-alcian blue whole-mount methods, counts of goblet cells were done in eight different localities in 1, 2, 5, 10, 20, 30, 50, and 100 fields measuring 0.01768 mm2. The median density and range of goblet cells by each mode of counting were compared and the mean deviation determined. Even as little as 2-5 blindly placed counts, which do not take much time, afford in a 4 mm2 area a fairly reliable orientation regarding density, considerably more reliable than an estimate of the density in sections, so often reported in the literature. Determination of the goblet cell density can be included as a routine method in the histopathological diagnosis of mucosal diseases of the upper and lower respiratory tract.", "contents": "Routine procedure for determining goblet cell density in the mucosa of the respiratory tract. In two normal nasal septa, fine-dissected and stained by PAS-alcian blue whole-mount methods, counts of goblet cells were done in eight different localities in 1, 2, 5, 10, 20, 30, 50, and 100 fields measuring 0.01768 mm2. The median density and range of goblet cells by each mode of counting were compared and the mean deviation determined. Even as little as 2-5 blindly placed counts, which do not take much time, afford in a 4 mm2 area a fairly reliable orientation regarding density, considerably more reliable than an estimate of the density in sections, so often reported in the literature. Determination of the goblet cell density can be included as a routine method in the histopathological diagnosis of mucosal diseases of the upper and lower respiratory tract.", "PMID": 970131}
{"id": "pubmed23n0032_7799", "title": "Use of preformed cavities in rabbits for the quantitation of leukocyte chemotaxis caused by bacterial lipopolysaccharides.", "content": "Wound chambers implanted subcutaneously in rabbits proved suitable for measurements of leukocyte chemotaxis. Injection of bacterial lipopolysaccharide (LPS) in the chambers the sixth day after implantation was followed by a marked increase of polymorphonuclear leukocytes in the wound chamber fluid, the number of which was dependent on the time after application of LPS. Up to a certain amount of LPS, the concentration of leukocytes in the chamber fluid was dose-dependent. The histopathological appearance of the granulation tissue lining the chamber wall one day after the injection of LPS from Veillonella revealed aggregation of blood cells plugging the lumina of small vessels and many eosinophilic leukocytes.", "contents": "Use of preformed cavities in rabbits for the quantitation of leukocyte chemotaxis caused by bacterial lipopolysaccharides. Wound chambers implanted subcutaneously in rabbits proved suitable for measurements of leukocyte chemotaxis. Injection of bacterial lipopolysaccharide (LPS) in the chambers the sixth day after implantation was followed by a marked increase of polymorphonuclear leukocytes in the wound chamber fluid, the number of which was dependent on the time after application of LPS. Up to a certain amount of LPS, the concentration of leukocytes in the chamber fluid was dose-dependent. The histopathological appearance of the granulation tissue lining the chamber wall one day after the injection of LPS from Veillonella revealed aggregation of blood cells plugging the lumina of small vessels and many eosinophilic leukocytes.", "PMID": 970134}
{"id": "pubmed23n0032_7800", "title": "The effect of ascorbic acid on production of human interferon and the antiviral activity in vitro.", "content": "The effects of ascorbic acid on interferon production and on the antiviral effect of interferon in cultures of human cells were investigated. Ascorbic acid enhanced the interferon levels produced by human embryo skin and human embryo lung fibroblasts, induced by Newcastle disease virus and by polyinosinic-polycytidylic acid. The same concentrations of ascorbic acid had no effect on interferon production in two lymphoblastoid cell lines induced by Sendai virus. Leucocyte interferon assayed in lung fibroblasts titrated 0.2-0.3 log10 units higher in the presence of 5 mug ascorbic acid than in the absence of the latter.", "contents": "The effect of ascorbic acid on production of human interferon and the antiviral activity in vitro. The effects of ascorbic acid on interferon production and on the antiviral effect of interferon in cultures of human cells were investigated. Ascorbic acid enhanced the interferon levels produced by human embryo skin and human embryo lung fibroblasts, induced by Newcastle disease virus and by polyinosinic-polycytidylic acid. The same concentrations of ascorbic acid had no effect on interferon production in two lymphoblastoid cell lines induced by Sendai virus. Leucocyte interferon assayed in lung fibroblasts titrated 0.2-0.3 log10 units higher in the presence of 5 mug ascorbic acid than in the absence of the latter.", "PMID": 970135}
{"id": "pubmed23n0032_7801", "title": "Immunochemical analysis of an unusual cell wall polysaccharide from animal coagulase-positive staphylococci. 1. Fragments obtained after hydrolysis in hydrofluoric acid and alkali.", "content": "Polysaccharide P (poly P) of canine coagulase-positive staphylococci contains glycerol, glucose, glucosamine, muramic acid, phosphate, and the usual peptidoglycan amino acids, but does not cross-react serologically with standard teichoic acids. Products from hydrolyses in hydrofluoric acid and alkali contained phosphates of glycerol and glucose as well as combinations of these, but neither glucosyl-glycerol units nor glucosamine-phosphates were observed. The teichoic acid of poly P is probably a polymer of a repeating unit consisting of alternating glycerol, phosphate and glucose.", "contents": "Immunochemical analysis of an unusual cell wall polysaccharide from animal coagulase-positive staphylococci. 1. Fragments obtained after hydrolysis in hydrofluoric acid and alkali. Polysaccharide P (poly P) of canine coagulase-positive staphylococci contains glycerol, glucose, glucosamine, muramic acid, phosphate, and the usual peptidoglycan amino acids, but does not cross-react serologically with standard teichoic acids. Products from hydrolyses in hydrofluoric acid and alkali contained phosphates of glycerol and glucose as well as combinations of these, but neither glucosyl-glycerol units nor glucosamine-phosphates were observed. The teichoic acid of poly P is probably a polymer of a repeating unit consisting of alternating glycerol, phosphate and glucose.", "PMID": 970136}
{"id": "pubmed23n0032_7802", "title": "Isolation of Yersinia enterocolitica from Danish swine and dogs.", "content": "Seventeen strains of Yersinia enterocolitica were isolated on examination of the caecal contents from 100 bacon pigs at slaughter. In another material consisting of 222 pigs with various diseases, the bacterium was found in 12 cases. Three out of 40 dogs were positive for the bacterium. Seven of the porcine and one of the canine strains belonged to serotype O 3, which is a human pathogen.", "contents": "Isolation of Yersinia enterocolitica from Danish swine and dogs. Seventeen strains of Yersinia enterocolitica were isolated on examination of the caecal contents from 100 bacon pigs at slaughter. In another material consisting of 222 pigs with various diseases, the bacterium was found in 12 cases. Three out of 40 dogs were positive for the bacterium. Seven of the porcine and one of the canine strains belonged to serotype O 3, which is a human pathogen.", "PMID": 970137}
{"id": "pubmed23n0032_7803", "title": "The critical transmural pressure of the abirway.", "content": "The critical transmural pressure (Ptm) is defined as the transmural pressure of the airway at the site where and when flow is limited during a forced expiration. According to the presented theory, the maximal expiratory flow (Vmax) can be calculated from the relation between Ptm and the corresponding cross-sectional area of the airway (A). By means of a pitot-static tube, Ptm-A curves were constructed for several locations in the elastic airway of a mechanical model. From these curves local Vmax was calculated at different values of Ptm and compared with actual flow, i.e. measured Vmax for the entire airway. In the downstream part of the airway, the actual flow equalled calculated Vmax at most Ptm values. The site of flow limitation, being the most upstream point where actual flow equals calculated local Vmax could therefore be located. Theory and experiments showed positive as well as negative Ptm not influenced by change in upstream or downstream resistance. Flow limitation could therefore be initiated at distending as well as compressing pressures across the wall of the airway. V was regarded as a function of Ptm and the elastic recoil pressure of the lung (Pel). Measured and calculated iso-Pel, Ptm-V curves agreed well with one major exception: when Ptm less than Ptm measured curves were distorted due to a concomitant downstrean compression of the collapsible airway.", "contents": "The critical transmural pressure of the abirway. The critical transmural pressure (Ptm) is defined as the transmural pressure of the airway at the site where and when flow is limited during a forced expiration. According to the presented theory, the maximal expiratory flow (Vmax) can be calculated from the relation between Ptm and the corresponding cross-sectional area of the airway (A). By means of a pitot-static tube, Ptm-A curves were constructed for several locations in the elastic airway of a mechanical model. From these curves local Vmax was calculated at different values of Ptm and compared with actual flow, i.e. measured Vmax for the entire airway. In the downstream part of the airway, the actual flow equalled calculated Vmax at most Ptm values. The site of flow limitation, being the most upstream point where actual flow equals calculated local Vmax could therefore be located. Theory and experiments showed positive as well as negative Ptm not influenced by change in upstream or downstream resistance. Flow limitation could therefore be initiated at distending as well as compressing pressures across the wall of the airway. V was regarded as a function of Ptm and the elastic recoil pressure of the lung (Pel). Measured and calculated iso-Pel, Ptm-V curves agreed well with one major exception: when Ptm less than Ptm measured curves were distorted due to a concomitant downstrean compression of the collapsible airway.", "PMID": 970143}
{"id": "pubmed23n0032_7804", "title": "Local reflex in microcirculation in human subcutaneous tissue.", "content": "The effect of changes in transmural pressure in the vessles of the extremities on blood flow in subcutaneous adipose tissue were investigated in three healthy subjects. Changes in transmural pressure were obtained either by postural changes of a limb, by locally induced subatmospheric pressure, or be venous stasis. Blood flow in subcutaneous tissue was measured 10 cm distal to the fibular head and at the lateral malleolus in the leg, or in the distal part of the forearm by the local 133Xenon washout technique. When transmural pressure increased 25 mmHg or more, blood flow decreased about 50 per cent due to an increase in vascular resconstrictor response to increase in transmural pressure could be blocked by infiltrating the area under study with phentolamine or lidocaine or by induced counterpressure. In these cases blood flow remained constant. The findings indicate that the vasoconstrictor response to increase in transmural pressure of 25 mmHg or more is due to a local nervous mechanism involving adrenergic nerves. The receptors are presumably stretch receptors placed in the small veins. The effector site is probably the arterioles. When the vasoconstrictor response is blocked, autoregulation of blood flow in subcutaneous tissue, i.e. maintenance of constant blood flow during changes in perfusion pressure head or during changes in transmural pressure, is revealed. The vasoconstrictor response to increase in transmural pressure will counteract an increase in transmural pressure in the exchange vessles and thereby act as an edema protecting factor.", "contents": "Local reflex in microcirculation in human subcutaneous tissue. The effect of changes in transmural pressure in the vessles of the extremities on blood flow in subcutaneous adipose tissue were investigated in three healthy subjects. Changes in transmural pressure were obtained either by postural changes of a limb, by locally induced subatmospheric pressure, or be venous stasis. Blood flow in subcutaneous tissue was measured 10 cm distal to the fibular head and at the lateral malleolus in the leg, or in the distal part of the forearm by the local 133Xenon washout technique. When transmural pressure increased 25 mmHg or more, blood flow decreased about 50 per cent due to an increase in vascular resconstrictor response to increase in transmural pressure could be blocked by infiltrating the area under study with phentolamine or lidocaine or by induced counterpressure. In these cases blood flow remained constant. The findings indicate that the vasoconstrictor response to increase in transmural pressure of 25 mmHg or more is due to a local nervous mechanism involving adrenergic nerves. The receptors are presumably stretch receptors placed in the small veins. The effector site is probably the arterioles. When the vasoconstrictor response is blocked, autoregulation of blood flow in subcutaneous tissue, i.e. maintenance of constant blood flow during changes in perfusion pressure head or during changes in transmural pressure, is revealed. The vasoconstrictor response to increase in transmural pressure will counteract an increase in transmural pressure in the exchange vessles and thereby act as an edema protecting factor.", "PMID": 970144}
{"id": "pubmed23n0032_7805", "title": "Hyperglycemic and hyperosmolar responses to graded hemorrhage.", "content": "Changes of the arterial plasma osmolality and of the glucose concentration were followed during a 30 min period of graded hemorrhagic hypotension (80, 50, and 30 mmHg) in the cat. Bleeding evoked a significant plasma hyperosmolality at all three hypotension levles and the responses were quantitatively related to the degree of hypotension. An approximate steady state increase in the arterial plasma osmolality was reached about 20 min after the start of the bleeding and it then averaged 8. 20, and 25 mOsm/kg H2O at 80, 50, and 30 mmHg, respectively. Bleeding also evoked an increase in the plasma glucose concentration, which almost entirely accounted for the observed hyperosmolality, especially at 80 and 50 mmHg. In late stages of hypotension at 30 mmHg, elevated plasma lactate and potassium concentrations contributed to the overall hyperosmolality. --Previous hemorrhagic hypotension experiments at 50 mmHg (J\u00e4rhult 1975 b) have shown that hyperosmolality serves as an important regulator of the plasma and extracellular fluid volumes during bleeding. The present results indicate that such an osmolar compensatory mechanism is operating over wide ranges of hemorrhagic hypotension.", "contents": "Hyperglycemic and hyperosmolar responses to graded hemorrhage. Changes of the arterial plasma osmolality and of the glucose concentration were followed during a 30 min period of graded hemorrhagic hypotension (80, 50, and 30 mmHg) in the cat. Bleeding evoked a significant plasma hyperosmolality at all three hypotension levles and the responses were quantitatively related to the degree of hypotension. An approximate steady state increase in the arterial plasma osmolality was reached about 20 min after the start of the bleeding and it then averaged 8. 20, and 25 mOsm/kg H2O at 80, 50, and 30 mmHg, respectively. Bleeding also evoked an increase in the plasma glucose concentration, which almost entirely accounted for the observed hyperosmolality, especially at 80 and 50 mmHg. In late stages of hypotension at 30 mmHg, elevated plasma lactate and potassium concentrations contributed to the overall hyperosmolality. --Previous hemorrhagic hypotension experiments at 50 mmHg (J\u00e4rhult 1975 b) have shown that hyperosmolality serves as an important regulator of the plasma and extracellular fluid volumes during bleeding. The present results indicate that such an osmolar compensatory mechanism is operating over wide ranges of hemorrhagic hypotension.", "PMID": 970145}
{"id": "pubmed23n0032_7806", "title": "Factors influencing the insulin space in cerebral cortex slices from adult and 7-day-old rats.", "content": "The inulin spaces and swelling of brain cortex slices from adult and 7-day-old rats were measured under differing experimental conditions. At 37 degrees C inulin penetrated into larger compartments than at 0 degrees C in both age groups. At 37 degrees C the inulin space increased with decreasing concentration of inulin in the medium. At 0 degrees C the inulin space did not depend on the inulin concentration. The swelling of the slices at 37 degrees C diminished with increasing concentrations of inulin. Anaerobic conditions, 0.2, and 1.0 mmol/l sodium cyanide and 0.1 mmol/l dinitrophenol reduced the inulin space and caused increased swelling of the slices. 1.0 mmol/l sodium iodoacetate was ineffective. In anaerobic conditions the inulin space did not change significantly as a function of the inulin concentration. A serious disadvantage of the inulin space as an indicator of extracellular space is that its size depends on the inulin concentration used. The inulin itself may influence the size of the space to be measured. Inulin also in osmotically inactive concentrations considerably reduces the swelling of brain cortex slices.", "contents": "Factors influencing the insulin space in cerebral cortex slices from adult and 7-day-old rats. The inulin spaces and swelling of brain cortex slices from adult and 7-day-old rats were measured under differing experimental conditions. At 37 degrees C inulin penetrated into larger compartments than at 0 degrees C in both age groups. At 37 degrees C the inulin space increased with decreasing concentration of inulin in the medium. At 0 degrees C the inulin space did not depend on the inulin concentration. The swelling of the slices at 37 degrees C diminished with increasing concentrations of inulin. Anaerobic conditions, 0.2, and 1.0 mmol/l sodium cyanide and 0.1 mmol/l dinitrophenol reduced the inulin space and caused increased swelling of the slices. 1.0 mmol/l sodium iodoacetate was ineffective. In anaerobic conditions the inulin space did not change significantly as a function of the inulin concentration. A serious disadvantage of the inulin space as an indicator of extracellular space is that its size depends on the inulin concentration used. The inulin itself may influence the size of the space to be measured. Inulin also in osmotically inactive concentrations considerably reduces the swelling of brain cortex slices.", "PMID": 970146}
{"id": "pubmed23n0032_7807", "title": "The potassium concentration in cerebrospinal fluid in young and adult rats following complete brain ischemia. Effects of pretreatment with hypoxia.", "content": "The potassium concentration in the cisternal cerebrospinal fluid (CSF) was measured following brain ischemia in rats of different ages which has been kept at normoxia or pretreated with hypoxia (PIO2 = 70 mmHg) for 24 h. In all age groups the potassium concentration rose following ischemia. The rate of rise was relatively slow in the 4-day rat and faster in 16- and 24-day rats; beyond this age the rate of rise became slower. Pretreatment with hypoxia significantly diminished the rate of rise in CSF potassium in 4- and 8-day rats, while no effect was observed in the older age groups. It is suggested that the rate of rise in CSF potassium is inversely correlated with the capacity of surviving a period of oxygen deprivation.", "contents": "The potassium concentration in cerebrospinal fluid in young and adult rats following complete brain ischemia. Effects of pretreatment with hypoxia. The potassium concentration in the cisternal cerebrospinal fluid (CSF) was measured following brain ischemia in rats of different ages which has been kept at normoxia or pretreated with hypoxia (PIO2 = 70 mmHg) for 24 h. In all age groups the potassium concentration rose following ischemia. The rate of rise was relatively slow in the 4-day rat and faster in 16- and 24-day rats; beyond this age the rate of rise became slower. Pretreatment with hypoxia significantly diminished the rate of rise in CSF potassium in 4- and 8-day rats, while no effect was observed in the older age groups. It is suggested that the rate of rise in CSF potassium is inversely correlated with the capacity of surviving a period of oxygen deprivation.", "PMID": 970147}
{"id": "pubmed23n0032_7808", "title": "The effect of veratrine and aconitine on the excitability of sensory units in the tooth of the cat.", "content": "Intradental sensory nerve impulse activity was recorded by means of electrodes inserted into dentinal cavities in the tooth of the cat. Impulses of different amplitudes was recorded. Local application of aconitine (10(-3) g/ml) and veratrine (10(-3) g/ml) induced nerve impulse activity. A rapid increase in the temperature of the tooth surface to 45-47 degrees C, which is known to induce fluid movements in the dentinal tubuli, did not induce any nerve impulse activity. However, after pretreatment of a tooth cavity with veratrine a rapid increase in the temperature of the tooth surface gave rise to nerve impulse activity. Steady state impulse activity induced by brief local application of aconitine was influenced by a rapid increase in the temperature of the tooth; during the increase in temperature impulses of low amplitude increased in frequency while impulses of high amplitude were blocked. Furthermore, reduced pressure applied to the exposed dentin, which also induces fluid movements in the dentin, gave rise to nerve impulse activity. Thus the sensory units in the tooth are stimulated by dentinal fluid movements and alkaloids known to excite mechanoreceptors increase their excitability. It is therefore suggested that the intradental sensory nerve endings are mechanosensitive structures.", "contents": "The effect of veratrine and aconitine on the excitability of sensory units in the tooth of the cat. Intradental sensory nerve impulse activity was recorded by means of electrodes inserted into dentinal cavities in the tooth of the cat. Impulses of different amplitudes was recorded. Local application of aconitine (10(-3) g/ml) and veratrine (10(-3) g/ml) induced nerve impulse activity. A rapid increase in the temperature of the tooth surface to 45-47 degrees C, which is known to induce fluid movements in the dentinal tubuli, did not induce any nerve impulse activity. However, after pretreatment of a tooth cavity with veratrine a rapid increase in the temperature of the tooth surface gave rise to nerve impulse activity. Steady state impulse activity induced by brief local application of aconitine was influenced by a rapid increase in the temperature of the tooth; during the increase in temperature impulses of low amplitude increased in frequency while impulses of high amplitude were blocked. Furthermore, reduced pressure applied to the exposed dentin, which also induces fluid movements in the dentin, gave rise to nerve impulse activity. Thus the sensory units in the tooth are stimulated by dentinal fluid movements and alkaloids known to excite mechanoreceptors increase their excitability. It is therefore suggested that the intradental sensory nerve endings are mechanosensitive structures.", "PMID": 970150}
{"id": "pubmed23n0032_7809", "title": "Decrease of oxygen consumption in the dog liver during temporary arterial occlusion.", "content": "The effects of occlusion of the hepatic artery on total and regional splanchnic oxygen consumption were studied in lightly anaesthetized dogs. Mean whole body oxygen uptake (+/- S.D.) was 4.72 +/- 0.55 ml/kg b.w. min-1, mean liver oxygen uptake (+/- S.D.) 1.18 +/- 0.42 ml/kg b.w. min-1 and mean oxygen uptake of the portally-drained tissues (+/- S.D.) was 0.80 +/- 0.54 ml/kg b.w. min-1 during the control period. The hepatic artery contributed 45 +/- 24% of the total liver oxygen uptake. The duration of occlusion was 45 min. Mean liver oxygen uptake was found to decrease to 64% of control values. The extraction of oxygen from the portal blood increased slightly. Mean whole body oxygen uptake and mean oxygen uptake of the portally-drained tissues were unchanged. 45 min after release of the hepatic artery occlusion, liver oxygen consumption had returned to control values. It is concluded that oxygen uptake in the liver is correlated to oxygen tension.", "contents": "Decrease of oxygen consumption in the dog liver during temporary arterial occlusion. The effects of occlusion of the hepatic artery on total and regional splanchnic oxygen consumption were studied in lightly anaesthetized dogs. Mean whole body oxygen uptake (+/- S.D.) was 4.72 +/- 0.55 ml/kg b.w. min-1, mean liver oxygen uptake (+/- S.D.) 1.18 +/- 0.42 ml/kg b.w. min-1 and mean oxygen uptake of the portally-drained tissues (+/- S.D.) was 0.80 +/- 0.54 ml/kg b.w. min-1 during the control period. The hepatic artery contributed 45 +/- 24% of the total liver oxygen uptake. The duration of occlusion was 45 min. Mean liver oxygen uptake was found to decrease to 64% of control values. The extraction of oxygen from the portal blood increased slightly. Mean whole body oxygen uptake and mean oxygen uptake of the portally-drained tissues were unchanged. 45 min after release of the hepatic artery occlusion, liver oxygen consumption had returned to control values. It is concluded that oxygen uptake in the liver is correlated to oxygen tension.", "PMID": 970151}
{"id": "pubmed23n0032_7810", "title": "A method to correct for the influence of gas density on maximal expiratory flow rate.", "content": "Maximal expiratory flow rate (Vmax) was measured at 20, 35, 50, 65, and 80% vital capacity in 4 young healthy subjects breathing air, SF6/O2, and He/O2 mixtures. The flows of SF6/O2 and He/O2 were corrected to normal alveolar gasflow by means of only the density of the gases. The values for normal alveolar gasflow and corrected SF6/O2 flow were identical at 35% VC and larger volumes while the values for normal alveolar gasflow and corrected He/O2 flow were not. The results indicate that in young healthy subjects it is possible to correct Vmax at lung volumes above 35% VC for the changes induced by an increase in density of the gas breathed, provided viscosity is not much changed. Without correction, Vmax after O2-breathing will be underestimated by about 6%, compared with Vmax for normal alveolar gas, whereas a change in alveolar CO2 concentrations between 3 and 9% only causes a 1% decrease of Vmax.", "contents": "A method to correct for the influence of gas density on maximal expiratory flow rate. Maximal expiratory flow rate (Vmax) was measured at 20, 35, 50, 65, and 80% vital capacity in 4 young healthy subjects breathing air, SF6/O2, and He/O2 mixtures. The flows of SF6/O2 and He/O2 were corrected to normal alveolar gasflow by means of only the density of the gases. The values for normal alveolar gasflow and corrected SF6/O2 flow were identical at 35% VC and larger volumes while the values for normal alveolar gasflow and corrected He/O2 flow were not. The results indicate that in young healthy subjects it is possible to correct Vmax at lung volumes above 35% VC for the changes induced by an increase in density of the gas breathed, provided viscosity is not much changed. Without correction, Vmax after O2-breathing will be underestimated by about 6%, compared with Vmax for normal alveolar gas, whereas a change in alveolar CO2 concentrations between 3 and 9% only causes a 1% decrease of Vmax.", "PMID": 970152}
{"id": "pubmed23n0032_7811", "title": "Renal renin output during continuous intracarotid infusions of iso- and hypertonic sodium chloride solutions in the rat.", "content": "On male Sprague Dawley rats isotonic NaCl during control and 1 M NaCl were infused either into the carotid artery or i.v. Glomerular filtration rate, sodium output and plasma renin activity were determined. Five of 19 animals reacted with no increase in sodium output for 1 M NaCl and are treated as a different group from the animals reacting with an increased sodium output. For the animals reacting with an increased sodium output a decrease in plasma renin activity was found together with an increase in glomerular filtration rate. In both groups the plasma sodium was constant. The animals not reacting with an increased sodium output had a higher initial plasma renin activity, which did not change during 1 M NaCl infusion. The responses were equal for both intracarotid and i.v. infusions but with a somewhat longer delay before the response occurred with i.v. infusions. These results might be explained by a central nervous effect and by a direct renal effect. Also during 1 M NaCl infusion a possible extracellular volume expansion, derived from water withdrawal from the cells into the extracellular space might occur.", "contents": "Renal renin output during continuous intracarotid infusions of iso- and hypertonic sodium chloride solutions in the rat. On male Sprague Dawley rats isotonic NaCl during control and 1 M NaCl were infused either into the carotid artery or i.v. Glomerular filtration rate, sodium output and plasma renin activity were determined. Five of 19 animals reacted with no increase in sodium output for 1 M NaCl and are treated as a different group from the animals reacting with an increased sodium output. For the animals reacting with an increased sodium output a decrease in plasma renin activity was found together with an increase in glomerular filtration rate. In both groups the plasma sodium was constant. The animals not reacting with an increased sodium output had a higher initial plasma renin activity, which did not change during 1 M NaCl infusion. The responses were equal for both intracarotid and i.v. infusions but with a somewhat longer delay before the response occurred with i.v. infusions. These results might be explained by a central nervous effect and by a direct renal effect. Also during 1 M NaCl infusion a possible extracellular volume expansion, derived from water withdrawal from the cells into the extracellular space might occur.", "PMID": 970153}
{"id": "pubmed23n0032_7812", "title": "Intrinsic regulation of blood flow in adipose tissue.", "content": "Previous studies on intact human subcutaneous tissue have shown, that blood flow remains constant during minor changes in perfusion pressure. This so-called autoregulatory response has not been demonstrable in isolated preparations of adipose tissue. In the present study on isolated, denervated subcutaneous tissue in female rabbits only 2 of 12 expts. revealed an autoregulatory response during reduction in arterial perfusion pressure. Effluent blood flow from the tissue in the control state was 15.5 ml/100 g-min (S.D. 6.4, n = 12) corresponding to slight vasodilatation of the exposed tissue. Following total ischemia all experiments showed a period with reactive hyperemia, and both duration of hyperemia and excess flow was related to the duration of the ischemia. This response therefore seems more resistant to the experimental procedure, while autoregulation of blood flow to lowered pressure is more susceptible to surgical exposure of the tissue. During elevation of arterial perfusion pressure blood flow in the isolated tissue showed a transient increase and then almost returned to the level during normotension, indicating an elevated vascular resistance. Raising of venous pressure elicited vasoconstriction with pronounced flow reduction. These two reactions may be important for local regulation of blood flow in subcutaneous tissue during orthostatic changes in arterial and venous pressure. It is concluded that the response in adipose tissue to changes in arterial pressure (autoregulation), venous pressure and total ischemia appear to be elicited by different mechanisms.", "contents": "Intrinsic regulation of blood flow in adipose tissue. Previous studies on intact human subcutaneous tissue have shown, that blood flow remains constant during minor changes in perfusion pressure. This so-called autoregulatory response has not been demonstrable in isolated preparations of adipose tissue. In the present study on isolated, denervated subcutaneous tissue in female rabbits only 2 of 12 expts. revealed an autoregulatory response during reduction in arterial perfusion pressure. Effluent blood flow from the tissue in the control state was 15.5 ml/100 g-min (S.D. 6.4, n = 12) corresponding to slight vasodilatation of the exposed tissue. Following total ischemia all experiments showed a period with reactive hyperemia, and both duration of hyperemia and excess flow was related to the duration of the ischemia. This response therefore seems more resistant to the experimental procedure, while autoregulation of blood flow to lowered pressure is more susceptible to surgical exposure of the tissue. During elevation of arterial perfusion pressure blood flow in the isolated tissue showed a transient increase and then almost returned to the level during normotension, indicating an elevated vascular resistance. Raising of venous pressure elicited vasoconstriction with pronounced flow reduction. These two reactions may be important for local regulation of blood flow in subcutaneous tissue during orthostatic changes in arterial and venous pressure. It is concluded that the response in adipose tissue to changes in arterial pressure (autoregulation), venous pressure and total ischemia appear to be elicited by different mechanisms.", "PMID": 970154}
{"id": "pubmed23n0032_7813", "title": "Influence of moderate vasoconstriction on the wave reflection properties of the pulmonary arterial bed.", "content": "Increased transmural pressure in the pulmonary arterial bed may reduce vascular input impedance and reduce hydraulic power linked to pulsatile blood flow. Vascular impedance and pulsatile hydraulic power (Wp) levels of isolated perfused rabbit lungs were compared after similar rises of pulmonary arterial pressure (PAp), induced either by vasoconstriction or by left atrial pressure (LAp) elevation. Resulting Wp levels were significantly smaller after vasoconstriction than LAp elevation. Wp showed a minimum level at physiologic PAp (about 20 cm H2O) irrespective of the cause of PAp elevation. Pressure pulse wave reflection coefficient (see article) was calculated for control and test situations, and was found to be approximately doubled after vasoconstriction. Only minor changes in (see article) were found after LAp elevation. Accordingly, moderate vasoconstriction (resulting PAp approximately 20 cm H2O) caused a backward traveling pressure wave of high amplitude, appearing in counter-phase to the forward pressure wave at the input site. The total pressure wave amplitude was thereby markedly lowered, resulting in a reduced Wp level. We assume that this effect of moderate vasoconstriction may be one reason for the existence of vascular smooth muscles in the pulmonary arteries.", "contents": "Influence of moderate vasoconstriction on the wave reflection properties of the pulmonary arterial bed. Increased transmural pressure in the pulmonary arterial bed may reduce vascular input impedance and reduce hydraulic power linked to pulsatile blood flow. Vascular impedance and pulsatile hydraulic power (Wp) levels of isolated perfused rabbit lungs were compared after similar rises of pulmonary arterial pressure (PAp), induced either by vasoconstriction or by left atrial pressure (LAp) elevation. Resulting Wp levels were significantly smaller after vasoconstriction than LAp elevation. Wp showed a minimum level at physiologic PAp (about 20 cm H2O) irrespective of the cause of PAp elevation. Pressure pulse wave reflection coefficient (see article) was calculated for control and test situations, and was found to be approximately doubled after vasoconstriction. Only minor changes in (see article) were found after LAp elevation. Accordingly, moderate vasoconstriction (resulting PAp approximately 20 cm H2O) caused a backward traveling pressure wave of high amplitude, appearing in counter-phase to the forward pressure wave at the input site. The total pressure wave amplitude was thereby markedly lowered, resulting in a reduced Wp level. We assume that this effect of moderate vasoconstriction may be one reason for the existence of vascular smooth muscles in the pulmonary arteries.", "PMID": 970155}
{"id": "pubmed23n0032_7814", "title": "The influence of pulmonary blood flow rate on vascular input impedance and hydraulic power in the sympathetically and noradrenaline stimulated cat lung.", "content": "This study was designed to evaluate the influence of sympathetic nerve stimulation (SN) and alpha-adrenergic receptor stimulation (alphaS) on the pulmonary vascular input impedance and hydraulic power output of the right heart during variations of cardiac output (CO). An open chest cat preparation was used and pulsatile pressure and flow in the pulmonary artery were measured by high frequency response transducers. Calculations showed that vascular resistance (VR) was inversely dependent on CO, but input impedance of the unstimulated lung was not influenced by CO variations. NS or alphaS increased VR and input impedance significantly, and the relation pulsatile hydraulic power/total hydraulic power (Wp/Wt) increased 40%, indicating that such stimulation has larger relative influence on impedance than on resistance. The reduction of arterial compliance during NS (maximal stimulus) was calculated to be 60%, independent of CO. Input impedance during NS or alphaS was reduced by CO elevations, probably because the concomitant distension of the arterial bed reduced arterial resistance and inertance. The ratio Wp/CO, which expresses the fraction of pulsatile hydraulic power lost per ml mean arterial flow, was found to be flow dependent both in control and stimulated conditions: Wp/CO was positively correlated to CO in control condition and weakly negatively correlated to CO during stimulation. At high CO the arterial vessels could be stimulated and stiffened without much extra load on the right heart.", "contents": "The influence of pulmonary blood flow rate on vascular input impedance and hydraulic power in the sympathetically and noradrenaline stimulated cat lung. This study was designed to evaluate the influence of sympathetic nerve stimulation (SN) and alpha-adrenergic receptor stimulation (alphaS) on the pulmonary vascular input impedance and hydraulic power output of the right heart during variations of cardiac output (CO). An open chest cat preparation was used and pulsatile pressure and flow in the pulmonary artery were measured by high frequency response transducers. Calculations showed that vascular resistance (VR) was inversely dependent on CO, but input impedance of the unstimulated lung was not influenced by CO variations. NS or alphaS increased VR and input impedance significantly, and the relation pulsatile hydraulic power/total hydraulic power (Wp/Wt) increased 40%, indicating that such stimulation has larger relative influence on impedance than on resistance. The reduction of arterial compliance during NS (maximal stimulus) was calculated to be 60%, independent of CO. Input impedance during NS or alphaS was reduced by CO elevations, probably because the concomitant distension of the arterial bed reduced arterial resistance and inertance. The ratio Wp/CO, which expresses the fraction of pulsatile hydraulic power lost per ml mean arterial flow, was found to be flow dependent both in control and stimulated conditions: Wp/CO was positively correlated to CO in control condition and weakly negatively correlated to CO during stimulation. At high CO the arterial vessels could be stimulated and stiffened without much extra load on the right heart.", "PMID": 970156}
{"id": "pubmed23n0032_7815", "title": "Influence of vessel distension and myogenic tone on pulmonary arterial input impedance. A study using a computer model of rabbit lung.", "content": "A computer model of the pulmonary arterial (PA) bed of rabbit lungs was designed in order to test experimental observations of changes in PA input impedance and pulsatile hydraulic power (cap.) during increased PA pressure. The computer model was based on a simple 3-component analog representation of single vessels (i.e. resistance, inertance and compliance). 16 generations of arterial vessels, from PA to 60 mum diameter, were combined to calculate PA input impedance. Input impedance was found to mimic closely that observed experimentally. Both venous pressure elevation and arteriolar constriction was found to reduce input impedance and Wp. By combining arteriolar constriction with increased myogenic tone of the larger arteries, Wp was found to show a minimum level at a certain PA pressure, dependent on the degree of arterial stiffening. Wp was found to follow changes in arterial volume and resistance during stimulated vasoconstriction. Wp dissipation in arterial vessels was calculated to approx. 50% of total imput Wp at physiological pressure conditions, and could be reduced by one half after PA pressure increase from 20 to 50 cm H2O, despite a concurrent halving of arterial compliance. Arterial vessels smaller than 200 mum diameter were found to have negligible direct influence on PA input impedance.", "contents": "Influence of vessel distension and myogenic tone on pulmonary arterial input impedance. A study using a computer model of rabbit lung. A computer model of the pulmonary arterial (PA) bed of rabbit lungs was designed in order to test experimental observations of changes in PA input impedance and pulsatile hydraulic power (cap.) during increased PA pressure. The computer model was based on a simple 3-component analog representation of single vessels (i.e. resistance, inertance and compliance). 16 generations of arterial vessels, from PA to 60 mum diameter, were combined to calculate PA input impedance. Input impedance was found to mimic closely that observed experimentally. Both venous pressure elevation and arteriolar constriction was found to reduce input impedance and Wp. By combining arteriolar constriction with increased myogenic tone of the larger arteries, Wp was found to show a minimum level at a certain PA pressure, dependent on the degree of arterial stiffening. Wp was found to follow changes in arterial volume and resistance during stimulated vasoconstriction. Wp dissipation in arterial vessels was calculated to approx. 50% of total imput Wp at physiological pressure conditions, and could be reduced by one half after PA pressure increase from 20 to 50 cm H2O, despite a concurrent halving of arterial compliance. Arterial vessels smaller than 200 mum diameter were found to have negligible direct influence on PA input impedance.", "PMID": 970157}
{"id": "pubmed23n0032_7816", "title": "Chemical sympathectomy of interscapular brown adipose tissue.", "content": "Adult non-cold adapted rats were injected with 6-hydroxydopamine (6-OHDA) or saline and their interscapular brown adipose tissue (BAT) was removed after appropriate periods of time. Fluorescence histochemistry of control BAT demonstrated the presence of an extensive network of varicose fibers among the adipocytes and at the blood vessels. This was confirmed by electron microscopy which also revealed large and small dense core vesicles sparsely distributed in axons and terminals indicating the presence of noradrenaline (NA). After 6-OHDA injection the fluorescence from varicosities was abolished both among the adipocytes and at the vessels. Thus, chemical sympathectomy was more effective than surgical- or immunosympathectomy, which spare the innervation of adipocytes. Parallelling the disappearance of fluorescence was a significant decrease of measurable NA. During recovery the extractable NA increased before the reappearance of fluorescent varicosities. This could be explained by transmitter accumulation in the nervetrunks within the tissue, which, in general, appeared unaffected by 6-OHDA. A large number of cells with a strong yellowish fluorescence distributed through the BAT was unaffected by 6-OHDA. There was no evidence for the presence of intrinsic ganglia.", "contents": "Chemical sympathectomy of interscapular brown adipose tissue. Adult non-cold adapted rats were injected with 6-hydroxydopamine (6-OHDA) or saline and their interscapular brown adipose tissue (BAT) was removed after appropriate periods of time. Fluorescence histochemistry of control BAT demonstrated the presence of an extensive network of varicose fibers among the adipocytes and at the blood vessels. This was confirmed by electron microscopy which also revealed large and small dense core vesicles sparsely distributed in axons and terminals indicating the presence of noradrenaline (NA). After 6-OHDA injection the fluorescence from varicosities was abolished both among the adipocytes and at the vessels. Thus, chemical sympathectomy was more effective than surgical- or immunosympathectomy, which spare the innervation of adipocytes. Parallelling the disappearance of fluorescence was a significant decrease of measurable NA. During recovery the extractable NA increased before the reappearance of fluorescent varicosities. This could be explained by transmitter accumulation in the nervetrunks within the tissue, which, in general, appeared unaffected by 6-OHDA. A large number of cells with a strong yellowish fluorescence distributed through the BAT was unaffected by 6-OHDA. There was no evidence for the presence of intrinsic ganglia.", "PMID": 970158}
{"id": "pubmed23n0032_7817", "title": "Inhibition of the lipolytic response to nerve stimulation during acidosis.", "content": "Acidosis inhibits catecholamine-induced lipolysis in vivo and in vitro. The lipolytic response of canine subcutaneous adipose tissue to short (5 min) nerve stimulations at 4 Hz was, however, not influenced by hypercapnic acidosis (pH 7.0). The steady state outflow of glycerol during a prolonged nerve stimulation at 4 Hz was inhibited by 40 per cent (p less than 0.05) at pH 7.0. Similarly, glycerol outflow during vasodilatation induced by a 4 Hz stimulation in alpha-blocked adipose tissue was inhibited by 37 per cent (p less than 0.05). Post-stimulatory glycerol outflow was, however, not influenced by acidosis. This poststimulatory glycerol outflow, which may represent a complex wash-out phenomenon, forms the largest part of the response to short nerve stimulations. It is suggested that steady state, rather than poststimulatory lipolysis should be studied in order to see the influence of treatments such as acidosis on responses to nerve stimulation.", "contents": "Inhibition of the lipolytic response to nerve stimulation during acidosis. Acidosis inhibits catecholamine-induced lipolysis in vivo and in vitro. The lipolytic response of canine subcutaneous adipose tissue to short (5 min) nerve stimulations at 4 Hz was, however, not influenced by hypercapnic acidosis (pH 7.0). The steady state outflow of glycerol during a prolonged nerve stimulation at 4 Hz was inhibited by 40 per cent (p less than 0.05) at pH 7.0. Similarly, glycerol outflow during vasodilatation induced by a 4 Hz stimulation in alpha-blocked adipose tissue was inhibited by 37 per cent (p less than 0.05). Post-stimulatory glycerol outflow was, however, not influenced by acidosis. This poststimulatory glycerol outflow, which may represent a complex wash-out phenomenon, forms the largest part of the response to short nerve stimulations. It is suggested that steady state, rather than poststimulatory lipolysis should be studied in order to see the influence of treatments such as acidosis on responses to nerve stimulation.", "PMID": 970159}
{"id": "pubmed23n0032_7818", "title": "Influence of prostaglandin E1 on cerebral mechanisms involved in the control of fluid balance.", "content": "The effects of infusions of PGE1 (30 ng/kg min-1) into the lateral cerebral ventricle were studied in the conscious, hydrated goat. The infusions caused release of antidiuretic hormone and increased renal sodium excretion. When PGE1 was infused together with hypertonic NaCl these effects became markedly enhanced and the infusion also induced drinking and a rise in the arterial blood pressure. Much weaker effects were obtained by the infusion of the hypertonic NaCl alone. This sodium-PGE1 interaction is discussed in relation to previously observed, central sodium-angiotensin II interaction. A more pronounced drinking effect was obtained in response to the intraventricular infusion of PGE1 + angiotensin II, than to the infusion of either substance separately. The PGE1 administered into the lateral cerebral ventricle did not induce any febrile response.", "contents": "Influence of prostaglandin E1 on cerebral mechanisms involved in the control of fluid balance. The effects of infusions of PGE1 (30 ng/kg min-1) into the lateral cerebral ventricle were studied in the conscious, hydrated goat. The infusions caused release of antidiuretic hormone and increased renal sodium excretion. When PGE1 was infused together with hypertonic NaCl these effects became markedly enhanced and the infusion also induced drinking and a rise in the arterial blood pressure. Much weaker effects were obtained by the infusion of the hypertonic NaCl alone. This sodium-PGE1 interaction is discussed in relation to previously observed, central sodium-angiotensin II interaction. A more pronounced drinking effect was obtained in response to the intraventricular infusion of PGE1 + angiotensin II, than to the infusion of either substance separately. The PGE1 administered into the lateral cerebral ventricle did not induce any febrile response.", "PMID": 970160}
{"id": "pubmed23n0032_7819", "title": "Gamma-aminobutyric acid release from neurons and glia.", "content": "Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes has been studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabelled GABA at micromolar concentrations markedly stimulated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a calcium-deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.", "contents": "Gamma-aminobutyric acid release from neurons and glia. Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes has been studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabelled GABA at micromolar concentrations markedly stimulated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a calcium-deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.", "PMID": 970161}
{"id": "pubmed23n0032_7820", "title": "[Early psychotherapy in posttraumatic syndromes. A case study].", "content": "The present study analyses one case of posttraumatic syndrome following an accident, which was apparently benign. The author studies neuropsychological investigations and improvement during psychotherapy, along with intellectual \"revalidation\". The emotional perturbation is accompanied by a dysfunction in mental efficiency, mostly memory. The improvement takes place both in the affective and intellectual areas. The possible hypothesis regarding the etiology of such perturbations are being reviewed. The author underlines the impact of the emotional trauma along with the subsequent perturbations in a patient already sensitised to previous accidents in her family.", "contents": "[Early psychotherapy in posttraumatic syndromes. A case study]. The present study analyses one case of posttraumatic syndrome following an accident, which was apparently benign. The author studies neuropsychological investigations and improvement during psychotherapy, along with intellectual \"revalidation\". The emotional perturbation is accompanied by a dysfunction in mental efficiency, mostly memory. The improvement takes place both in the affective and intellectual areas. The possible hypothesis regarding the etiology of such perturbations are being reviewed. The author underlines the impact of the emotional trauma along with the subsequent perturbations in a patient already sensitised to previous accidents in her family.", "PMID": 970181}
{"id": "pubmed23n0032_7821", "title": "[Premonitory psychiatric signs of cardiovascular complaints].", "content": "The present study attempts to find out whether 19 behaviors, identified in 1952 and considered as revealing of a psychoneurotic state, appear more often among subjects who complain, 16 years later (in 1968), of a cardiovascular problem than among the subjects who remain free of any cardiovascular complaint during this period. The sample analysed comes from an epidemiological survey, realized by Leighton and known by the name Stirling County Study. That study includes 654 subjects free of any cardiovascular complaint in 1952. These subjects were divided into five independent categories in terms of the presence or absence of certain cardiovascular complaints expressed in 1968. The five categories of cardiovascular complaints were compared as to the frequency and nature of the psychiatric characteristics which differentiate them. Three conclusions appear from this study. 1. The psychiatric characteristics (identified in 1952) are more numerous among the subjects who manifest, 16 years later, a cardiovascular complaint (identified in 1968), when one compares them to the psychiatric characteristics of those who do not develop any cardiovascular complaint during this period. 2. The patterns of psychiatric characteristics which differentiate the subjects who develop a cardiovascular complaint vary with sex. These patterns are much more broad, coherent, and differentiating among women. 3. The accumulation of psychiatric characteristics possesses, among women, a differentiating value greater than any characteristic taken alone.", "contents": "[Premonitory psychiatric signs of cardiovascular complaints]. The present study attempts to find out whether 19 behaviors, identified in 1952 and considered as revealing of a psychoneurotic state, appear more often among subjects who complain, 16 years later (in 1968), of a cardiovascular problem than among the subjects who remain free of any cardiovascular complaint during this period. The sample analysed comes from an epidemiological survey, realized by Leighton and known by the name Stirling County Study. That study includes 654 subjects free of any cardiovascular complaint in 1952. These subjects were divided into five independent categories in terms of the presence or absence of certain cardiovascular complaints expressed in 1968. The five categories of cardiovascular complaints were compared as to the frequency and nature of the psychiatric characteristics which differentiate them. Three conclusions appear from this study. 1. The psychiatric characteristics (identified in 1952) are more numerous among the subjects who manifest, 16 years later, a cardiovascular complaint (identified in 1968), when one compares them to the psychiatric characteristics of those who do not develop any cardiovascular complaint during this period. 2. The patterns of psychiatric characteristics which differentiate the subjects who develop a cardiovascular complaint vary with sex. These patterns are much more broad, coherent, and differentiating among women. 3. The accumulation of psychiatric characteristics possesses, among women, a differentiating value greater than any characteristic taken alone.", "PMID": 970180}
{"id": "pubmed23n0032_7822", "title": "High doses of Haldol in the treatment of 5 young schizophrenics in a therapeutic community.", "content": "Even within a unique setting with high-powered and board-spectrum therapeutic tools, acute agitation can pose a serious problem. In this communication we review the cases of five schizophrenic adolescents whose agitation created a threat to themselves and the therapeutic community. The institute tried a variety of psychotherapeutic and chemotherapeutic approaches to no avail and only as last resort we used Haldol in very high doses. Since research centers which specialise in the study of schizophrenic persons and their families rarely publish about chemotherapy in high doses, it seems worthwhile relating our findings.", "contents": "High doses of Haldol in the treatment of 5 young schizophrenics in a therapeutic community. Even within a unique setting with high-powered and board-spectrum therapeutic tools, acute agitation can pose a serious problem. In this communication we review the cases of five schizophrenic adolescents whose agitation created a threat to themselves and the therapeutic community. The institute tried a variety of psychotherapeutic and chemotherapeutic approaches to no avail and only as last resort we used Haldol in very high doses. Since research centers which specialise in the study of schizophrenic persons and their families rarely publish about chemotherapy in high doses, it seems worthwhile relating our findings.", "PMID": 970183}
{"id": "pubmed23n0032_7823", "title": "[Long-acting neuroleptics. IV. Preliminary study of clopimozide (R 29764)].", "content": "Clopimozide is, in our opinion, a noteworthy neuroleptic drug. Its action is mainly ataraxic but also antidelusional, long-acting and accompanied with minimal side-effects. It is therefore particularly indicated in the maintenance therapy of psychotics, at a weekly dosage of 7.5 to 25 mg. The present conclusions are preliminary because of the limited number of patients (10) but also because of the rigidity of the trial protocol suggested to us.", "contents": "[Long-acting neuroleptics. IV. Preliminary study of clopimozide (R 29764)]. Clopimozide is, in our opinion, a noteworthy neuroleptic drug. Its action is mainly ataraxic but also antidelusional, long-acting and accompanied with minimal side-effects. It is therefore particularly indicated in the maintenance therapy of psychotics, at a weekly dosage of 7.5 to 25 mg. The present conclusions are preliminary because of the limited number of patients (10) but also because of the rigidity of the trial protocol suggested to us.", "PMID": 970182}
{"id": "pubmed23n0032_7824", "title": "Differential fertility of adopted schizophrenics and their half-siblings.", "content": "The paper presents data on the differential fertility of schizophrenics and controls, and the fertility of their siblings. This study used several methodological procedures in the study of schizophrenia reproduction, which strengthens the validity of the findings. Firstly, both male and female rates were examined. Secondly, the method of selection of a control avoided the biases introduced by using census data or other non-matched controls. Third, a diagnostic criterion was used which minimizes the possibility of the inclusion of other psychiatric illnesses. The results obtained support prior reports of the lowered reproductive rates of schizophrenics. Further, the siblings of schizophrenics were found not to have a reproductive advantage when contrasted to control siblings. The failure to find a reproductive advantage conflicts with a hypothesis of a balanced polymorphism as the mechanism maintaining an apparent constant rate of schizophrenia.", "contents": "Differential fertility of adopted schizophrenics and their half-siblings. The paper presents data on the differential fertility of schizophrenics and controls, and the fertility of their siblings. This study used several methodological procedures in the study of schizophrenia reproduction, which strengthens the validity of the findings. Firstly, both male and female rates were examined. Secondly, the method of selection of a control avoided the biases introduced by using census data or other non-matched controls. Third, a diagnostic criterion was used which minimizes the possibility of the inclusion of other psychiatric illnesses. The results obtained support prior reports of the lowered reproductive rates of schizophrenics. Further, the siblings of schizophrenics were found not to have a reproductive advantage when contrasted to control siblings. The failure to find a reproductive advantage conflicts with a hypothesis of a balanced polymorphism as the mechanism maintaining an apparent constant rate of schizophrenia.", "PMID": 970192}
{"id": "pubmed23n0032_7825", "title": "[Introduction to the epidemiological study of five years of extra-hospital psychosocial practice at Ixelles].", "content": "This work is aimed at encompassing five years (1969-1973) of extra-hospital psychosocial activity performed by a multidisciplinary team of the sector of the population of Ixelles (85.000 inhabitants). Apart from a population study, we have focused on all consultants (676 patients) in a statistical frame. We have defined three directions: 1. Obtaining a complete picture of the consulting population according to epidemiological and clinical variables. 2. The control in our daily practice of three classical goals which underline the work in sector (permanence -- multidisciplinary team work -- priority to psychotics). 3. The attempt to verify some common assertions in psychiatry, general and social: relationship between season and pathology, between obligation to consults (for the patient) and social class, between type of pathology and social class. Our results for the two last issues differ from those of Hollingshead and Redlich.", "contents": "[Introduction to the epidemiological study of five years of extra-hospital psychosocial practice at Ixelles]. This work is aimed at encompassing five years (1969-1973) of extra-hospital psychosocial activity performed by a multidisciplinary team of the sector of the population of Ixelles (85.000 inhabitants). Apart from a population study, we have focused on all consultants (676 patients) in a statistical frame. We have defined three directions: 1. Obtaining a complete picture of the consulting population according to epidemiological and clinical variables. 2. The control in our daily practice of three classical goals which underline the work in sector (permanence -- multidisciplinary team work -- priority to psychotics). 3. The attempt to verify some common assertions in psychiatry, general and social: relationship between season and pathology, between obligation to consults (for the patient) and social class, between type of pathology and social class. Our results for the two last issues differ from those of Hollingshead and Redlich.", "PMID": 970186}
{"id": "pubmed23n0032_7826", "title": "Sleep deprivation as treatment for endogenous depression.", "content": "Twenty-six depressed patients, 19 of whom suffered from an endogenous depression, were sleep-deprived for one night, and eight of these were additionally sleep-deprived for three to nine nights with two sleep deprivations per week. While the sleep deprivation was being carried through, none of the patients were treated with tricyclic antidepressants. The patients were rated before and after the sleep deprivation(s). Sleep deprivation appeared to be effective for both unipolar and bipolar depressions. According to the rating scale an improvement was registered especially when the clinical picture was characterized by depressed mood, psycho-motor retardation and anxiety. As sleep deprivation cured 25% of the patients and further incidentally improved another 20% of the patients, it can be concluded that sleep deprivation seems to be a valuable treatment, especially in retarded endogenous depressions.", "contents": "Sleep deprivation as treatment for endogenous depression. Twenty-six depressed patients, 19 of whom suffered from an endogenous depression, were sleep-deprived for one night, and eight of these were additionally sleep-deprived for three to nine nights with two sleep deprivations per week. While the sleep deprivation was being carried through, none of the patients were treated with tricyclic antidepressants. The patients were rated before and after the sleep deprivation(s). Sleep deprivation appeared to be effective for both unipolar and bipolar depressions. According to the rating scale an improvement was registered especially when the clinical picture was characterized by depressed mood, psycho-motor retardation and anxiety. As sleep deprivation cured 25% of the patients and further incidentally improved another 20% of the patients, it can be concluded that sleep deprivation seems to be a valuable treatment, especially in retarded endogenous depressions.", "PMID": 970193}
{"id": "pubmed23n0032_7827", "title": "Pipamperone (Dipiperon) in the treatment of behaviour disorders. A large-scale multicentre evaluation.", "content": "Pipamperone solution or tablets were prescribed for four weeks to 188 patients (3-100 years) with behavior disorders. Fifty of these (group A) were mental retardates, the remaining 138 (group B) had a normal I.Q. Their doses were gradually increased until optimal effect was reached. Nearly all 17 items of a behaviour rating scale had improved very significantly by the end of the fourth week, while in 82% of the patients of group A and 76% of group B results were rated good to very good. Also, pipamperone proved to be far superior to previous psychotropic drugs in patients who had been treated before.", "contents": "Pipamperone (Dipiperon) in the treatment of behaviour disorders. A large-scale multicentre evaluation. Pipamperone solution or tablets were prescribed for four weeks to 188 patients (3-100 years) with behavior disorders. Fifty of these (group A) were mental retardates, the remaining 138 (group B) had a normal I.Q. Their doses were gradually increased until optimal effect was reached. Nearly all 17 items of a behaviour rating scale had improved very significantly by the end of the fourth week, while in 82% of the patients of group A and 76% of group B results were rated good to very good. Also, pipamperone proved to be far superior to previous psychotropic drugs in patients who had been treated before.", "PMID": 970185}
{"id": "pubmed23n0032_7828", "title": "[Socioecohomic data on 447 hospitalized former prisoners of war].", "content": "The authors have analyzed the social files of all former POWs hospitalized at the Ste-Ode Hospital (Belgium) during one year. They found numerous significant results, that might be the subject of further analyses: i.a., frequent professionally inactive people, despite their age; 5 more times divorces than in the general belgian population; neglect of mental disorders in the attribution of a war invalidity.", "contents": "[Socioecohomic data on 447 hospitalized former prisoners of war]. The authors have analyzed the social files of all former POWs hospitalized at the Ste-Ode Hospital (Belgium) during one year. They found numerous significant results, that might be the subject of further analyses: i.a., frequent professionally inactive people, despite their age; 5 more times divorces than in the general belgian population; neglect of mental disorders in the attribution of a war invalidity.", "PMID": 970189}
{"id": "pubmed23n0032_7829", "title": "Urbanization and psychiatric disorders--the Hong Kong scene.", "content": "Indices were used to portray the rapidity and intensity of urbanization Hong Kong had undergone in the past 2-3 decades. Next the preliminary result of a psychiatric survey was quoted as an estimate of mental ill-health in Hong Kong. Data were obtained from the Mental Health Service to show the increasing demand for psychiatric care for the past 25 years. Attempts were made to analyze the basic composition of the new patients attending a psychiatric centre during this period. High density living associated with urbanization in Hong Kong was found to generate a high level of emotional strain, and psychiatric ill-health was as prevalent as that in New York. Neuroses were on the increase and psychiatrists were being consulted on a wider variety of problems. Provision of mental health care in future should take the changes in the composition of psychiatric populations into consideration.", "contents": "Urbanization and psychiatric disorders--the Hong Kong scene. Indices were used to portray the rapidity and intensity of urbanization Hong Kong had undergone in the past 2-3 decades. Next the preliminary result of a psychiatric survey was quoted as an estimate of mental ill-health in Hong Kong. Data were obtained from the Mental Health Service to show the increasing demand for psychiatric care for the past 25 years. Attempts were made to analyze the basic composition of the new patients attending a psychiatric centre during this period. High density living associated with urbanization in Hong Kong was found to generate a high level of emotional strain, and psychiatric ill-health was as prevalent as that in New York. Neuroses were on the increase and psychiatrists were being consulted on a wider variety of problems. Provision of mental health care in future should take the changes in the composition of psychiatric populations into consideration.", "PMID": 970194}
{"id": "pubmed23n0032_7830", "title": "[Psychotic masked depression or black mask for depression].", "content": "We have used as depression criteria those of Pichot and Hassan, described during the Sint-Moritz Symposium in 1973 on masked depression. According to their clinical experience in Za\u00efre and Senegal, the authors consider the possibility that the \"bouff\u00e9e d\u00e9lirante\" (acute psychotic reaction), frequent in black Africa, is in fact a manifestation of depression. They remind the five criterias of Pichot and Hassan, i.e.: 1 degree evidence of depression symptoms; 2 degrees a background and a particular underlying personality; 3 degrees evolutional characteristics; 4 degrees familial antecedents and hereditary  factors; 5 degrees the response to antidepressive treatment. They present ten cases of acute psychosis: five from Za\u00efre and five from Senegal. These ten patients have been successfully treated with antidepressive drugs imipramine type, without any neuroleptic drugs; the outcome has always been a rapid remission. Some socio-cultural references are described; it permits to better comprehend the psychological frame of African people. An attempt to psychodynamically interpret the results ends the article.", "contents": "[Psychotic masked depression or black mask for depression]. We have used as depression criteria those of Pichot and Hassan, described during the Sint-Moritz Symposium in 1973 on masked depression. According to their clinical experience in Za\u00efre and Senegal, the authors consider the possibility that the \"bouff\u00e9e d\u00e9lirante\" (acute psychotic reaction), frequent in black Africa, is in fact a manifestation of depression. They remind the five criterias of Pichot and Hassan, i.e.: 1 degree evidence of depression symptoms; 2 degrees a background and a particular underlying personality; 3 degrees evolutional characteristics; 4 degrees familial antecedents and hereditary  factors; 5 degrees the response to antidepressive treatment. They present ten cases of acute psychosis: five from Za\u00efre and five from Senegal. These ten patients have been successfully treated with antidepressive drugs imipramine type, without any neuroleptic drugs; the outcome has always been a rapid remission. Some socio-cultural references are described; it permits to better comprehend the psychological frame of African people. An attempt to psychodynamically interpret the results ends the article.", "PMID": 970187}
{"id": "pubmed23n0032_7831", "title": "Sleep deprivation therapy in depression.", "content": "A study of sleep deprivation therapy was made in 62 females and 15 males, aged 20-72, with monopolar (60 patients) and bipolar (17 patients) types of manic-depressive psychosis. Of these patients, 30 had suffered only the current depression, 29 a maximum of five depressions, and 18 more than five depressions before the sleep deprivation therapy. Twenty-five patients had been treated with antidepressant drugs for less than 10 days, 12 patients for 10-24 days, and 36 patients for more than 24 days. Twenty-four patients were treated with one sleep deprivation, 29 patients with one sleep deprivations per week (average 1.59), and 24 patients with two sleep deprivations per week (average 2.5). The effect of the sleep deprivation therapy was evaluated clinically and by means of Cronholm-Ottosson's rating scale. The effect was found good and lasting in 29%, good but temporary in 38%, and poor in 32% of the cases. The best results were achieved with twice-weekly treatments, the poorest results with once-weekly treatment. The results were equal in monopolar and bipolar cases and were independent of the number of previous depressions as well as antidepressant drug treatment. No side effects have been observed, in particular no conversion to mania. The results of the present investigation indicate that depression and sleep disturbances are symptoms produced by a common factor which, however, it as yet unknown. Sleep deprivation therapy is seen to have at least some effect on all cases of endogenous depression. Sleep deprivation therapy has no side effects and is more quick-acting than any other treatment procedure hitherto known. It should therefore be considered the first treatment offer to all endogenously depressed patients in whom immediate ECT is not necessitated.", "contents": "Sleep deprivation therapy in depression. A study of sleep deprivation therapy was made in 62 females and 15 males, aged 20-72, with monopolar (60 patients) and bipolar (17 patients) types of manic-depressive psychosis. Of these patients, 30 had suffered only the current depression, 29 a maximum of five depressions, and 18 more than five depressions before the sleep deprivation therapy. Twenty-five patients had been treated with antidepressant drugs for less than 10 days, 12 patients for 10-24 days, and 36 patients for more than 24 days. Twenty-four patients were treated with one sleep deprivation, 29 patients with one sleep deprivations per week (average 1.59), and 24 patients with two sleep deprivations per week (average 2.5). The effect of the sleep deprivation therapy was evaluated clinically and by means of Cronholm-Ottosson's rating scale. The effect was found good and lasting in 29%, good but temporary in 38%, and poor in 32% of the cases. The best results were achieved with twice-weekly treatments, the poorest results with once-weekly treatment. The results were equal in monopolar and bipolar cases and were independent of the number of previous depressions as well as antidepressant drug treatment. No side effects have been observed, in particular no conversion to mania. The results of the present investigation indicate that depression and sleep disturbances are symptoms produced by a common factor which, however, it as yet unknown. Sleep deprivation therapy is seen to have at least some effect on all cases of endogenous depression. Sleep deprivation therapy has no side effects and is more quick-acting than any other treatment procedure hitherto known. It should therefore be considered the first treatment offer to all endogenously depressed patients in whom immediate ECT is not necessitated.", "PMID": 970195}
{"id": "pubmed23n0032_7832", "title": "[Environmental and demographic variables associated with psychiatric morbidity in former prisoners of war].", "content": "In this study, present psychiatric morbidity of 100 former prisoners of war is related to 28 environmental and demographic variables grouped in 3 periods: before, during and after WW2. With the exception of the invalidity percentage, all statistically significant relations concern variables of the first two periods. The present results are discussed at the light of former publications by the authors. It appears among others that the age variable has to be taken in consideration in the explanation of psychiatric sequels of captivity, and that the condition \"invalid of war\" poorly reflects the degree of psychiatric morbidity.", "contents": "[Environmental and demographic variables associated with psychiatric morbidity in former prisoners of war]. In this study, present psychiatric morbidity of 100 former prisoners of war is related to 28 environmental and demographic variables grouped in 3 periods: before, during and after WW2. With the exception of the invalidity percentage, all statistically significant relations concern variables of the first two periods. The present results are discussed at the light of former publications by the authors. It appears among others that the age variable has to be taken in consideration in the explanation of psychiatric sequels of captivity, and that the condition \"invalid of war\" poorly reflects the degree of psychiatric morbidity.", "PMID": 970190}
{"id": "pubmed23n0032_7833", "title": "[Severe neuropsychic disturbances in a young adolescent treated with an endocrine therapy].", "content": "After a treatment based on the administration of gonadotropic drugs, aimed at reduced school difficulties and caracterial difficulties by a premature onset of puberty, a young male aged 13 1/2 presents: 1 degree somatic anomalies; 2 degrees increase of intellectual, affective and behavioral troubles; 3 degrees perturbations of EEG prophiles; 4 degrees organicity tests significantly disturbed. The interruption of treatment (4 years of observation) was followed by a progressive remission, however heterogenous, of all initial troubles. The observation underlines the dangers of utilising these treatments in a pre-puberty boy.", "contents": "[Severe neuropsychic disturbances in a young adolescent treated with an endocrine therapy]. After a treatment based on the administration of gonadotropic drugs, aimed at reduced school difficulties and caracterial difficulties by a premature onset of puberty, a young male aged 13 1/2 presents: 1 degree somatic anomalies; 2 degrees increase of intellectual, affective and behavioral troubles; 3 degrees perturbations of EEG prophiles; 4 degrees organicity tests significantly disturbed. The interruption of treatment (4 years of observation) was followed by a progressive remission, however heterogenous, of all initial troubles. The observation underlines the dangers of utilising these treatments in a pre-puberty boy.", "PMID": 970191}
{"id": "pubmed23n0032_7834", "title": "What happened later to the lithium babies? A follow-up study of children born without malformations.", "content": "The Lithium Baby Register was founded in 1968 to determine the frequency of abnormalities among children born to mothers who were given lithium during the first trimester of pregnancy. Previous studies have revealed an increased frequency of congenital malformations, possibly due to teratogenic action of lithium. The present report is a questionnaire follow-up of the physical and mental development of lithium children who were not malformed at birth. Sixty lithium children were examined; their siblings, who had not been exposed to lithium during fetal life, served as a control group. The data obtained do not reveal any increased frequency of physical or mental anomalies among the lithium children.", "contents": "What happened later to the lithium babies? A follow-up study of children born without malformations. The Lithium Baby Register was founded in 1968 to determine the frequency of abnormalities among children born to mothers who were given lithium during the first trimester of pregnancy. Previous studies have revealed an increased frequency of congenital malformations, possibly due to teratogenic action of lithium. The present report is a questionnaire follow-up of the physical and mental development of lithium children who were not malformed at birth. Sixty lithium children were examined; their siblings, who had not been exposed to lithium during fetal life, served as a control group. The data obtained do not reveal any increased frequency of physical or mental anomalies among the lithium children.", "PMID": 970196}
{"id": "pubmed23n0032_7835", "title": "Self-image and emotional stability of Oedipal and non-Oedipal male homosexuals.", "content": "The past several years have brought about some changes in the legal status of the homosexual in USA. In some states, the homosexual is labelled as a criminal; in others, homosexuality is not a legal affair. Psychological theories tend to consider the homosexual from two general positions: the Oedipal and the non-Oedipal. The present study was conducted to try to determine how Oedipal and non-Oedipal male homosexuals perceived their self-image and emotional stability. Significant differences were found between the two groups. Considering self-image, Oedipal subjects were found to be more negative. They had less self-worth, self-confidence and self-acceptance than non-Oedipal. They had more negative self-concepts, self-attitudes, and self-motivation. Oedipal homosexuals had less emotional stability. They had more emotional problems, shame, guilt, withdrawal, and depression than non-Oedipal subjects, as well as less success in sexual activity and commitment to the homosexual partner.", "contents": "Self-image and emotional stability of Oedipal and non-Oedipal male homosexuals. The past several years have brought about some changes in the legal status of the homosexual in USA. In some states, the homosexual is labelled as a criminal; in others, homosexuality is not a legal affair. Psychological theories tend to consider the homosexual from two general positions: the Oedipal and the non-Oedipal. The present study was conducted to try to determine how Oedipal and non-Oedipal male homosexuals perceived their self-image and emotional stability. Significant differences were found between the two groups. Considering self-image, Oedipal subjects were found to be more negative. They had less self-worth, self-confidence and self-acceptance than non-Oedipal. They had more negative self-concepts, self-attitudes, and self-motivation. Oedipal homosexuals had less emotional stability. They had more emotional problems, shame, guilt, withdrawal, and depression than non-Oedipal subjects, as well as less success in sexual activity and commitment to the homosexual partner.", "PMID": 970188}
{"id": "pubmed23n0032_7836", "title": "Spinal cord vascularity. IV. The spinal cord arteries in the rat.", "content": "The present investigation has shown that the anatomy of the spinal cord arteries in the rat and in man is closely related but not identical. The main differences are: (1) The poor supply of radicular arteries to the lower cervical and upper thoracic segments of the cord is more marked in the rat, (2) the great ventral radicular artery of Adamkiewicz is less subjected to variations in position in the rat, (3) penetrating branches from the pial arterial plexus are absent in the rat, (4) surface anastomoses between the ventral and the dorsal spinal arteries do not occur in the rat except occasionally at the lower end of the cord. Intramedullary arterial anastomoses in the lumbosacral cord of the rat seems to be species specific.", "contents": "Spinal cord vascularity. IV. The spinal cord arteries in the rat. The present investigation has shown that the anatomy of the spinal cord arteries in the rat and in man is closely related but not identical. The main differences are: (1) The poor supply of radicular arteries to the lower cervical and upper thoracic segments of the cord is more marked in the rat, (2) the great ventral radicular artery of Adamkiewicz is less subjected to variations in position in the rat, (3) penetrating branches from the pial arterial plexus are absent in the rat, (4) surface anastomoses between the ventral and the dorsal spinal arteries do not occur in the rat except occasionally at the lower end of the cord. Intramedullary arterial anastomoses in the lumbosacral cord of the rat seems to be species specific.", "PMID": 970203}
{"id": "pubmed23n0032_7837", "title": "Computer tomography of the neurocranium.", "content": "The experience with computer tomography of the neurocranium in 300 patients submitted for computer tomography of the brain is reported. The more appropriate projections which may be obtained with the second generation of scanners in combination with an elaborated reconstruction technique seem to constitute a replacement of conventional skull films.", "contents": "Computer tomography of the neurocranium. The experience with computer tomography of the neurocranium in 300 patients submitted for computer tomography of the brain is reported. The more appropriate projections which may be obtained with the second generation of scanners in combination with an elaborated reconstruction technique seem to constitute a replacement of conventional skull films.", "PMID": 970204}
{"id": "pubmed23n0032_7838", "title": "Computer tomography in the evaluation of pulmonary asbestosis. Preliminary experiences with the EMI general purpose scanner.", "content": "Thirteen individuals with varying lengths of exposure to asbestosis were examined by computer tomography with the EMI Scanner. Various pleuroparenchymal abnormalities were found, many not being seen on standard chest films. In some cases, with normal chest radiography, definite pleural involvement could be demonstrated, particularly that in the posterior sulci on the diaphragm and at the mediastinum. Tissue attenuation measurements in regions of parenchymal involvement reached 90 EMI units above normal lung tissue. The normal gravity effect is abolished in cases of parenchymal disease caused by asbestosis. Parenchymal disease was much more obvious on the CT scans than on conventional chest films.", "contents": "Computer tomography in the evaluation of pulmonary asbestosis. Preliminary experiences with the EMI general purpose scanner. Thirteen individuals with varying lengths of exposure to asbestosis were examined by computer tomography with the EMI Scanner. Various pleuroparenchymal abnormalities were found, many not being seen on standard chest films. In some cases, with normal chest radiography, definite pleural involvement could be demonstrated, particularly that in the posterior sulci on the diaphragm and at the mediastinum. Tissue attenuation measurements in regions of parenchymal involvement reached 90 EMI units above normal lung tissue. The normal gravity effect is abolished in cases of parenchymal disease caused by asbestosis. Parenchymal disease was much more obvious on the CT scans than on conventional chest films.", "PMID": 970205}
{"id": "pubmed23n0032_7839", "title": "Pulmonary congestion in chronic heart disease. Radiologic, clinical and hemodynamic relationships.", "content": "Pulmonary congestion in chronic heart disease was evaluated by lung auscultation and roentgen examination of the chest and compared with pressure recording of the pulmonary circulation in 49 cases. Whereas auscultation appeared not to be correlated to the pressures, the radiologic findings proved to be a reliable measure of increased pressures. The radiologic assessment was based on isolated flow shift to the upper lobes of the lungs, indicating slight pulmonary venous hypertension, and flow shift in association with blurring of the lower lobe vessels, indicating pulmonary interstitial oedema. In distinction to the results from acute cardiac failure interlobular septa were often demonstrated in association with pulmonary interstitial oedema, and pulmonary alveolar oedema rarely demonstrated in spite of marked pulmonary venous hypertension.", "contents": "Pulmonary congestion in chronic heart disease. Radiologic, clinical and hemodynamic relationships. Pulmonary congestion in chronic heart disease was evaluated by lung auscultation and roentgen examination of the chest and compared with pressure recording of the pulmonary circulation in 49 cases. Whereas auscultation appeared not to be correlated to the pressures, the radiologic findings proved to be a reliable measure of increased pressures. The radiologic assessment was based on isolated flow shift to the upper lobes of the lungs, indicating slight pulmonary venous hypertension, and flow shift in association with blurring of the lower lobe vessels, indicating pulmonary interstitial oedema. In distinction to the results from acute cardiac failure interlobular septa were often demonstrated in association with pulmonary interstitial oedema, and pulmonary alveolar oedema rarely demonstrated in spite of marked pulmonary venous hypertension.", "PMID": 970206}
{"id": "pubmed23n0032_7840", "title": "Visibility of the intrapericardiac segment of the ascending aorta on conventional lateral chest films.", "content": "In a retrospective survey of the lateral chest films of 284 adult patients the intrapericardiac part of the ascending aorta was at least partly discernible in more than half of the cases; those with visible calcifications in the aortic wall were excluded. None of the patients had known heart disease. When the intrapericardiac part of the ascending aorta is visible it can be clinically useful for the localisation of intracardiac calcifications in relation to the aortic orifice (inside or outside), for estimating the sagittal diameter of the left atrium and for evaluating the width and shape of the aortic root.", "contents": "Visibility of the intrapericardiac segment of the ascending aorta on conventional lateral chest films. In a retrospective survey of the lateral chest films of 284 adult patients the intrapericardiac part of the ascending aorta was at least partly discernible in more than half of the cases; those with visible calcifications in the aortic wall were excluded. None of the patients had known heart disease. When the intrapericardiac part of the ascending aorta is visible it can be clinically useful for the localisation of intracardiac calcifications in relation to the aortic orifice (inside or outside), for estimating the sagittal diameter of the left atrium and for evaluating the width and shape of the aortic root.", "PMID": 970207}
{"id": "pubmed23n0032_7841", "title": "Effects on venous vasomotion from the ionic content of contrast agent solutions. An investigation on microcirculation in the bat wing.", "content": "The effects on spontaneous venous vasomotion from topical application of different contrast media were investigated in the bat wing. Spontaneous venous vasomotion could be stopped either by a high osmolality of the contrast medium or by absence of cations (Na+, K+, Ca++, Mg++) in the solution. The highest contrast medium concentration (mg I/ml), which did not stop venous vasomotion, was obtained when a non-ionic contrast medium solution with low osmolality was mixed with a solution containing Na, K, Ca, Mg-salts of an ionic medium.", "contents": "Effects on venous vasomotion from the ionic content of contrast agent solutions. An investigation on microcirculation in the bat wing. The effects on spontaneous venous vasomotion from topical application of different contrast media were investigated in the bat wing. Spontaneous venous vasomotion could be stopped either by a high osmolality of the contrast medium or by absence of cations (Na+, K+, Ca++, Mg++) in the solution. The highest contrast medium concentration (mg I/ml), which did not stop venous vasomotion, was obtained when a non-ionic contrast medium solution with low osmolality was mixed with a solution containing Na, K, Ca, Mg-salts of an ionic medium.", "PMID": 970208}
{"id": "pubmed23n0032_7842", "title": "Implantation metastasis after percutaneous transthoracic needle aspiration biopsy.", "content": "The risk of tumour spread at aspiration biopsy of pulmonary tumours through the needle track has been analyzed by reviewing the case histories of 1 264 patients with a malignant tumour demonstrated by needle biopsy. Only in one case did metastatic tumour growth develop at the site of the biopsy. The history of this case is presented and the available literature on the subject discussed.", "contents": "Implantation metastasis after percutaneous transthoracic needle aspiration biopsy. The risk of tumour spread at aspiration biopsy of pulmonary tumours through the needle track has been analyzed by reviewing the case histories of 1 264 patients with a malignant tumour demonstrated by needle biopsy. Only in one case did metastatic tumour growth develop at the site of the biopsy. The history of this case is presented and the available literature on the subject discussed.", "PMID": 970209}
{"id": "pubmed23n0032_7843", "title": "Catheterization and angiography of the abdominal arteries in the guinea-pig.", "content": "The abdominal arterial anatomy of the guinea-pig was investigated by angiography and dissection. Normally, a coeliacomesenteric trunk was found, although various forms transitional to the hepatolienogastric trunk were found. Multiple renal arteries were common in comparison to other laboratory animals and to man. A technique for selective catheterization of the abdominal arteries is described.", "contents": "Catheterization and angiography of the abdominal arteries in the guinea-pig. The abdominal arterial anatomy of the guinea-pig was investigated by angiography and dissection. Normally, a coeliacomesenteric trunk was found, although various forms transitional to the hepatolienogastric trunk were found. Multiple renal arteries were common in comparison to other laboratory animals and to man. A technique for selective catheterization of the abdominal arteries is described.", "PMID": 970210}
{"id": "pubmed23n0032_7844", "title": "Hydro-and hemodynamic effects of catheterization of vessels. I. An experimental model.", "content": "A mechanical model for experiments on the influence on pressure and volumetric flow rate arising from catheterization is described. It is possible under controlled conditions to determine the static and dynamic pressure together with the volumetric flow rate as a function of flow type, repetition frequency, shape and amplitude of pressure surges in pulsatile flow, peripheral resistance, geometry and dimensions of a stenosis or a catheter, and physical properties of the vessel wall materials and of the fluid employed.", "contents": "Hydro-and hemodynamic effects of catheterization of vessels. I. An experimental model. A mechanical model for experiments on the influence on pressure and volumetric flow rate arising from catheterization is described. It is possible under controlled conditions to determine the static and dynamic pressure together with the volumetric flow rate as a function of flow type, repetition frequency, shape and amplitude of pressure surges in pulsatile flow, peripheral resistance, geometry and dimensions of a stenosis or a catheter, and physical properties of the vessel wall materials and of the fluid employed.", "PMID": 970211}
{"id": "pubmed23n0032_7845", "title": "Kinematic analysis of posterolateral fusion in the lumbosacral spine.", "content": "Three cases operated upon with posterolateral fusion in the lumbosacral spine have been subjected to a kinematic postoperative analysis with the aid of a roentgen stereophotogrammetric method. The primarily diseased intervals became comparatively rigid in spite of significant residual mobility, in two of the patients, not until 174 days postoperatively. Three levels of operation displayed unrestricted residual mobility.", "contents": "Kinematic analysis of posterolateral fusion in the lumbosacral spine. Three cases operated upon with posterolateral fusion in the lumbosacral spine have been subjected to a kinematic postoperative analysis with the aid of a roentgen stereophotogrammetric method. The primarily diseased intervals became comparatively rigid in spite of significant residual mobility, in two of the patients, not until 174 days postoperatively. Three levels of operation displayed unrestricted residual mobility.", "PMID": 970212}
{"id": "pubmed23n0032_7846", "title": "Chronic multifocal cleido-metaphyseal osteomyelitis of childhood. Report of a case.", "content": "A case of chronic, multifocal hematogenous osteomyelitis in a child is descirbed. It represents a special, well-defined clinical variant of this disease, which obviously reflects a more or less balanced relationship between the bacterial aggressor and the defense mechanisms. Exacerbations of old processes, and spread to new sites, can be ascribed to a temporary imbalance.", "contents": "Chronic multifocal cleido-metaphyseal osteomyelitis of childhood. Report of a case. A case of chronic, multifocal hematogenous osteomyelitis in a child is descirbed. It represents a special, well-defined clinical variant of this disease, which obviously reflects a more or less balanced relationship between the bacterial aggressor and the defense mechanisms. Exacerbations of old processes, and spread to new sites, can be ascribed to a temporary imbalance.", "PMID": 970213}
{"id": "pubmed23n0032_7847", "title": "Radiologic differentiation between cysts and malignant tumours of the maxilla.", "content": "Analysis of 41 cases with large maxillary lesions (23 malignant tumours, 13 cysts and 5 other lesions) with the aid of special criteria based on the radiologic appearances of various lesions to distinguish cysts from malignant tumours and inflammatory processes.", "contents": "Radiologic differentiation between cysts and malignant tumours of the maxilla. Analysis of 41 cases with large maxillary lesions (23 malignant tumours, 13 cysts and 5 other lesions) with the aid of special criteria based on the radiologic appearances of various lesions to distinguish cysts from malignant tumours and inflammatory processes.", "PMID": 970214}
{"id": "pubmed23n0032_7848", "title": "Multiple expanding renal lesions.", "content": "Unilateral or bilateral multiplicity of expanding lesions in the kidneys is not too unusual and different types of lesions may be combined. This stresses the need for detailed angiography of both kidneys in order to establish an exact diagnosis.", "contents": "Multiple expanding renal lesions. Unilateral or bilateral multiplicity of expanding lesions in the kidneys is not too unusual and different types of lesions may be combined. This stresses the need for detailed angiography of both kidneys in order to establish an exact diagnosis.", "PMID": 970215}
{"id": "pubmed23n0032_7849", "title": "Clinical course after mantle treatment of non-laparotomized patients with Hodgkin's disease.", "content": "Forty-two patients with Hodgkin's disease were staged without laparotomy and considered to have only supradiaphragmatic disease. They were treated with the mantle technique and followed up for at least 3 years. Abdominal extension was later found in 31 per cent. It is concluded that occult abdominal disease usually in course of time will give symptoms.", "contents": "Clinical course after mantle treatment of non-laparotomized patients with Hodgkin's disease. Forty-two patients with Hodgkin's disease were staged without laparotomy and considered to have only supradiaphragmatic disease. They were treated with the mantle technique and followed up for at least 3 years. Abdominal extension was later found in 31 per cent. It is concluded that occult abdominal disease usually in course of time will give symptoms.", "PMID": 970216}
{"id": "pubmed23n0032_7850", "title": "Hypopharyngeal carcinoma. Long-term survivors following radical radiation therapy.", "content": "In a material of 167 cases with squamous cell carcinoma of the hypopharynx, treated between 1961 and 1971, radical irradiation was administered to 55 patients. Of these latter patients the 3- and 5-year survival rates were 36 and 25 per cent, respectively. The lesions of the upper part of the hypopharynx were found to be more curable than those of the lower part. Three cases are presented in detail.", "contents": "Hypopharyngeal carcinoma. Long-term survivors following radical radiation therapy. In a material of 167 cases with squamous cell carcinoma of the hypopharynx, treated between 1961 and 1971, radical irradiation was administered to 55 patients. Of these latter patients the 3- and 5-year survival rates were 36 and 25 per cent, respectively. The lesions of the upper part of the hypopharynx were found to be more curable than those of the lower part. Three cases are presented in detail.", "PMID": 970217}
{"id": "pubmed23n0032_7851", "title": "Effects of ionizing radiation on the activity of the ciliated epithelium of the trachea.", "content": "The immediate effect of ionizing radiation on the activity of the ciliated epithelium of the trachea has been investigated using a light reflection method. This method enables continuous registration of the mucociliary activity both during and after irradiation. A notable increase of the mucociliary activity occurred within 5 seconds after beginning of irradiation and this activity reached its maximum 10 seconds after initial exposure with a dose rate of 0.34 Gy/s (34 rad/s). The mechanism causing the phenomena observed is not clear but theoretically it might be due to ATP, the source of energy of the cilia being freed by the irradiation, possibly through disturbances of the permeability in the mitochondrion membranes.", "contents": "Effects of ionizing radiation on the activity of the ciliated epithelium of the trachea. The immediate effect of ionizing radiation on the activity of the ciliated epithelium of the trachea has been investigated using a light reflection method. This method enables continuous registration of the mucociliary activity both during and after irradiation. A notable increase of the mucociliary activity occurred within 5 seconds after beginning of irradiation and this activity reached its maximum 10 seconds after initial exposure with a dose rate of 0.34 Gy/s (34 rad/s). The mechanism causing the phenomena observed is not clear but theoretically it might be due to ATP, the source of energy of the cilia being freed by the irradiation, possibly through disturbances of the permeability in the mitochondrion membranes.", "PMID": 970218}
{"id": "pubmed23n0032_7852", "title": "Fall in blood pressure during radiation therapy.", "content": "Blood pressure and heart rate at rest in the supine and standing positions were followed before, during and after irradiation for malignant tumours in 114 patients. A statistically significant gradual reduction in blood pressure during the treatment period was established. This was more marked in older patients and in patients with higher initial blood pressure but was not related to the region irradiated or the type of tumour treated. Particularly if the patient experiences vertigo and nausea on change of position, it seems advisable to check the blood pressure during treatment.", "contents": "Fall in blood pressure during radiation therapy. Blood pressure and heart rate at rest in the supine and standing positions were followed before, during and after irradiation for malignant tumours in 114 patients. A statistically significant gradual reduction in blood pressure during the treatment period was established. This was more marked in older patients and in patients with higher initial blood pressure but was not related to the region irradiated or the type of tumour treated. Particularly if the patient experiences vertigo and nausea on change of position, it seems advisable to check the blood pressure during treatment.", "PMID": 970219}
{"id": "pubmed23n0032_7853", "title": "Absorbed dose in mammary radiography.", "content": "By exposure measurements in an alcohol-water mixture simulating breast tissue, the absorbed dose, and dose distribution in the breast at the radiation qualities commonly used in mammary radiography have been calculated. The absorbed doses for different recording media have been compared at those radiation qualities which result in similar image qualities in the different recording media.", "contents": "Absorbed dose in mammary radiography. By exposure measurements in an alcohol-water mixture simulating breast tissue, the absorbed dose, and dose distribution in the breast at the radiation qualities commonly used in mammary radiography have been calculated. The absorbed doses for different recording media have been compared at those radiation qualities which result in similar image qualities in the different recording media.", "PMID": 970220}
{"id": "pubmed23n0032_7854", "title": "Attenuation equalizing filter in diagnostic radiography. Advantages calculated by a Monte Carlo technique.", "content": "The value of an attenuation equalizing filter (equalizer) has been examined by calculation using a Monte Carlo technique. The contrast enhancement caused by the equalizer is the net result of three factors: (1) The first factor arises from the contrast change caused by the difference in detector characteristics at the different energies absorbed in the detector with and without equalizer. (2) The second factor is due to the contrast change caused by the different ratios of primary to total radiation reaching the detector. (3) Finally, the third factor describes how the transparency of the object is changed by the different primary filtration with equalizer. An example is given of the reduction in radiation energy absorbed by the patient and the question of how far the attenuation equalization can be driven is discussed.", "contents": "Attenuation equalizing filter in diagnostic radiography. Advantages calculated by a Monte Carlo technique. The value of an attenuation equalizing filter (equalizer) has been examined by calculation using a Monte Carlo technique. The contrast enhancement caused by the equalizer is the net result of three factors: (1) The first factor arises from the contrast change caused by the difference in detector characteristics at the different energies absorbed in the detector with and without equalizer. (2) The second factor is due to the contrast change caused by the different ratios of primary to total radiation reaching the detector. (3) Finally, the third factor describes how the transparency of the object is changed by the different primary filtration with equalizer. An example is given of the reduction in radiation energy absorbed by the patient and the question of how far the attenuation equalization can be driven is discussed.", "PMID": 970221}
{"id": "pubmed23n0032_7855", "title": "Diabetes mellitus and acute myocardial infarction.", "content": "A series of 597 consecutive patients with acute myocardial infarction (AMI) have been screened for diabetes mellitus (DM). Six per cent of the series had DM, which is exactly the frequency of DM in an age-matched population. This finding corresponds with results of other investigators, indicating that treated diabetics do not have an increased risk of AMI. Diabetics suffering from AMI do not have an increased mortality, nor do patients treated with oral antidiabetics have a higher mortality than those treated with insulin.", "contents": "Diabetes mellitus and acute myocardial infarction. A series of 597 consecutive patients with acute myocardial infarction (AMI) have been screened for diabetes mellitus (DM). Six per cent of the series had DM, which is exactly the frequency of DM in an age-matched population. This finding corresponds with results of other investigators, indicating that treated diabetics do not have an increased risk of AMI. Diabetics suffering from AMI do not have an increased mortality, nor do patients treated with oral antidiabetics have a higher mortality than those treated with insulin.", "PMID": 970222}
{"id": "pubmed23n0032_7856", "title": "Skeletal lesions of the feet in diabetics and their relationship to cutaneous erythema with or without necrosis on the feet.", "content": "Seventy patients with cutaneous erythema of the feet with or without necrosis were the subjects of this investigation. Sixty-five of them had open diabetes. The glucose tolerance of the remaining five patients was altered in a diabetic direction. Twenty-seven of the 70 patients had roentgenologically demonstrable destruction in the bones of the feet. These 70 patients were compared with 61 diabetic control patients of corresponding age and duration of diabetes but without these skin lesions of the feet. Only four of the 61 control patients had destruction in the bones of the feet and all these destructions were small. Precipitating factors were identified in general for the skin lesions, the most common being cardiac decompensation. A higher frequency of precipitating factors was seen in patients with skeletal destructions than in those without. The skeletal destructions and cutaneous necrosis are supposed to be equivalent lesions, localized to different tissues in the feet. When patients presenting skin lesions of the feet in the form of distal gangrene were compared with those who had cutaneous erythema and necrosis of the feet, but no distal gangrene, no differences were found with respect to age, duration of diabetes, occurrence of precipitating factors and the occurrence of skeletal destruction. Cutaneous erythema without necrosis is understood to be incipient diabetic gangrene.", "contents": "Skeletal lesions of the feet in diabetics and their relationship to cutaneous erythema with or without necrosis on the feet. Seventy patients with cutaneous erythema of the feet with or without necrosis were the subjects of this investigation. Sixty-five of them had open diabetes. The glucose tolerance of the remaining five patients was altered in a diabetic direction. Twenty-seven of the 70 patients had roentgenologically demonstrable destruction in the bones of the feet. These 70 patients were compared with 61 diabetic control patients of corresponding age and duration of diabetes but without these skin lesions of the feet. Only four of the 61 control patients had destruction in the bones of the feet and all these destructions were small. Precipitating factors were identified in general for the skin lesions, the most common being cardiac decompensation. A higher frequency of precipitating factors was seen in patients with skeletal destructions than in those without. The skeletal destructions and cutaneous necrosis are supposed to be equivalent lesions, localized to different tissues in the feet. When patients presenting skin lesions of the feet in the form of distal gangrene were compared with those who had cutaneous erythema and necrosis of the feet, but no distal gangrene, no differences were found with respect to age, duration of diabetes, occurrence of precipitating factors and the occurrence of skeletal destruction. Cutaneous erythema without necrosis is understood to be incipient diabetic gangrene.", "PMID": 970223}
{"id": "pubmed23n0032_7857", "title": "Body composition and glucose metabolism in hypertensive middle-aged males.", "content": "Body fat, body cell mass, fasting blood sugar, glucose tolerance and fasting insulin have been determined in 106 hypertensive males aged 47-54years and in 41 normotensive 50-year-old males. Both groups were derived from screening examinations in random population samples. The hypertensive subjects were more often obese and had more often an impaired glucose tolerance and a higher fasting insulin compared with the normotensive subjects. The metabolic differences were not explained simply by the higher degree of obesity in the hypertensive subjects, as the differences remained when the hypertensive subjects were matched for body fat with normotensive controls. The impaired glucose metabolism demonstrated quantitatively in an unselected group of hypertensive subjects, might be one of the factors explaining the variable prognosis in hypertensive subjects.", "contents": "Body composition and glucose metabolism in hypertensive middle-aged males. Body fat, body cell mass, fasting blood sugar, glucose tolerance and fasting insulin have been determined in 106 hypertensive males aged 47-54years and in 41 normotensive 50-year-old males. Both groups were derived from screening examinations in random population samples. The hypertensive subjects were more often obese and had more often an impaired glucose tolerance and a higher fasting insulin compared with the normotensive subjects. The metabolic differences were not explained simply by the higher degree of obesity in the hypertensive subjects, as the differences remained when the hypertensive subjects were matched for body fat with normotensive controls. The impaired glucose metabolism demonstrated quantitatively in an unselected group of hypertensive subjects, might be one of the factors explaining the variable prognosis in hypertensive subjects.", "PMID": 970224}
{"id": "pubmed23n0032_7858", "title": "Hereditary hepatic porphyrias in Finland.", "content": "The occurrence of hepatic porphyrias--acute intermittent porphyria (AIP) and variegate porphyria (VP)--in Finland has been studied. During a period of 9 years 107 patients with AIP and 45 patients with VP were found. The prevalence of hereditary hepatic porphyrias was calculated to be 3.4 per 100 000 inhabitants. The patients belonged to 42 different families. Eighty-nine patients (59%) had had acute attacks, whereas 63 were symptomless latent cases. Precipitating factors, symptoms and excretion of porphyrins and their precursors did not significantly differ from what has been reported earlier from other parts of the world. A slight fragility of the skin on the back of the hands was noted in some 50% of VP patients. Abnormal sensitivity to sunlight could not be seen in a single case. However, about 50% of patients with VP showed an abnormal reaction when irradiated with artificial ultraviolet light. The difference in the skin symptoms in South African and Finnish VP patients is discussed.", "contents": "Hereditary hepatic porphyrias in Finland. The occurrence of hepatic porphyrias--acute intermittent porphyria (AIP) and variegate porphyria (VP)--in Finland has been studied. During a period of 9 years 107 patients with AIP and 45 patients with VP were found. The prevalence of hereditary hepatic porphyrias was calculated to be 3.4 per 100 000 inhabitants. The patients belonged to 42 different families. Eighty-nine patients (59%) had had acute attacks, whereas 63 were symptomless latent cases. Precipitating factors, symptoms and excretion of porphyrins and their precursors did not significantly differ from what has been reported earlier from other parts of the world. A slight fragility of the skin on the back of the hands was noted in some 50% of VP patients. Abnormal sensitivity to sunlight could not be seen in a single case. However, about 50% of patients with VP showed an abnormal reaction when irradiated with artificial ultraviolet light. The difference in the skin symptoms in South African and Finnish VP patients is discussed.", "PMID": 970225}
{"id": "pubmed23n0032_7859", "title": "Pure mitral regurgitation. Etiology, pathology and clinical patterns.", "content": "The varied etiology of pure mitral regurgitation is demonstrated in this clinicopathological study, comprising 59 surgically treated cases with this condition. One third of the cases was of rheumatic origin, one fifth had ischemic heart disease, another fifth floppy valves and one eighth an isolated rupture of the chordae with necrosis of the chord matrix. To our knowledge the histopathological findings in the last group have not been described before. Congenital mitral regurgitation, bacterial endocarditis and cardiomyopathy were rare causes of mitral regurgitation. Differences between the groups were observed in the sex ratio, duration of history, auscultatory findings ECG signs, compliance of the left ventricle and in the morphological findings.", "contents": "Pure mitral regurgitation. Etiology, pathology and clinical patterns. The varied etiology of pure mitral regurgitation is demonstrated in this clinicopathological study, comprising 59 surgically treated cases with this condition. One third of the cases was of rheumatic origin, one fifth had ischemic heart disease, another fifth floppy valves and one eighth an isolated rupture of the chordae with necrosis of the chord matrix. To our knowledge the histopathological findings in the last group have not been described before. Congenital mitral regurgitation, bacterial endocarditis and cardiomyopathy were rare causes of mitral regurgitation. Differences between the groups were observed in the sex ratio, duration of history, auscultatory findings ECG signs, compliance of the left ventricle and in the morphological findings.", "PMID": 970226}
{"id": "pubmed23n0032_7860", "title": "Disopyramide in ventricular tachycardia.", "content": "Administration of disopyramide phosphate (DE) i.v. in two doses, 30 min apart, to a patient with ventricular tachycardia was accompanied by no, or only slight, changes in systemic arterial pressure (SAP), cardiac output (Q), stroke work (SW), and pulmonary artery diastolic pressure (PADP). Heart rate fell from 123 to 103/min. Following reversion to sinus rhythm, which occurred 60 min after the second dose of DE at a serum concentration greater than 4.3 mug/ml, Q ans SW showed significant increases above their control values. PADP fell from 20 to 6 mmHg whereas the mean SAP remained largely unchanged. There seemed to be no adverse effects of drug administration. In this patient, recurrent attacks of ventricular tachycardia not responding to conventional antiarrhythmic treatment could be prevented by oral DE in a dose of 800 mg/day.", "contents": "Disopyramide in ventricular tachycardia. Administration of disopyramide phosphate (DE) i.v. in two doses, 30 min apart, to a patient with ventricular tachycardia was accompanied by no, or only slight, changes in systemic arterial pressure (SAP), cardiac output (Q), stroke work (SW), and pulmonary artery diastolic pressure (PADP). Heart rate fell from 123 to 103/min. Following reversion to sinus rhythm, which occurred 60 min after the second dose of DE at a serum concentration greater than 4.3 mug/ml, Q ans SW showed significant increases above their control values. PADP fell from 20 to 6 mmHg whereas the mean SAP remained largely unchanged. There seemed to be no adverse effects of drug administration. In this patient, recurrent attacks of ventricular tachycardia not responding to conventional antiarrhythmic treatment could be prevented by oral DE in a dose of 800 mg/day.", "PMID": 970227}
{"id": "pubmed23n0032_7861", "title": "Clinical and hemodynamic findings following prosthetic valve replacement for mitral valve disease. A study of patients with the new Bj\u00f6rk-Shiley tilting disc valve.", "content": "Clinical and hemodynamic results have been evaluated 12--24 months after mitral valve replacement with the new Bj\u00f6rk-Shiley tilting disc valve prosthesis. After operation, most patients were improved symptomatically and were classified as I-II (N.Y.H.A.). No patient became worse. Hemodynamic status at rest showed significant reduction in pulmonary capillary venous pressure, pulmonary artial pressure and significant increase in cardiac output when compared with the preoperative values, but postoperative hemodynamic abnormalities remained. Exercise produced a rise in pressures in the pulmonary circuit and in cardiac output. The increase in cardiac output was less than expected from the increase in oxygen consumption, with a few exceptions. Apparently, there was no close relationship between the symptomatic improvement and the hemodynamic results. Thus, the present study points to the importance of hemodynamic data in the objective assessment of the results of cardiac surgery.", "contents": "Clinical and hemodynamic findings following prosthetic valve replacement for mitral valve disease. A study of patients with the new Bj\u00f6rk-Shiley tilting disc valve. Clinical and hemodynamic results have been evaluated 12--24 months after mitral valve replacement with the new Bj\u00f6rk-Shiley tilting disc valve prosthesis. After operation, most patients were improved symptomatically and were classified as I-II (N.Y.H.A.). No patient became worse. Hemodynamic status at rest showed significant reduction in pulmonary capillary venous pressure, pulmonary artial pressure and significant increase in cardiac output when compared with the preoperative values, but postoperative hemodynamic abnormalities remained. Exercise produced a rise in pressures in the pulmonary circuit and in cardiac output. The increase in cardiac output was less than expected from the increase in oxygen consumption, with a few exceptions. Apparently, there was no close relationship between the symptomatic improvement and the hemodynamic results. Thus, the present study points to the importance of hemodynamic data in the objective assessment of the results of cardiac surgery.", "PMID": 970228}
{"id": "pubmed23n0032_7862", "title": "A validation of cause-of-death certification in 1,156 deaths.", "content": "Swedish twins have been followed for mortality since 1961, when the Swedish Twin Registry was formed. During the years 1961-73 there were 1290 deaths among twins born in 1901-25. In 1156 cases the cause of death could be established from collected records and classified according to the 1965 revision of ICD. Using the review of records as the standard, rates of detection and confirmation relating to the death certificate diagnoses were calculated. It is concluded that Swedish death certificate data are fairly valid for use in epidemiological studies and mortality statistics with regard to most cancer forms, cerebrovascular disease, ischemic heart disease, bronchitis, asthma and emphysema, accidents and suicides, but not for diabetes mellitus, alcoholism, mental diseases, rheumatic heart diseases and other heart diseases. However, in selected clinical-epidemiological studies it is often necessary to collect all available documents prior to judging the cause of death.", "contents": "A validation of cause-of-death certification in 1,156 deaths. Swedish twins have been followed for mortality since 1961, when the Swedish Twin Registry was formed. During the years 1961-73 there were 1290 deaths among twins born in 1901-25. In 1156 cases the cause of death could be established from collected records and classified according to the 1965 revision of ICD. Using the review of records as the standard, rates of detection and confirmation relating to the death certificate diagnoses were calculated. It is concluded that Swedish death certificate data are fairly valid for use in epidemiological studies and mortality statistics with regard to most cancer forms, cerebrovascular disease, ischemic heart disease, bronchitis, asthma and emphysema, accidents and suicides, but not for diabetes mellitus, alcoholism, mental diseases, rheumatic heart diseases and other heart diseases. However, in selected clinical-epidemiological studies it is often necessary to collect all available documents prior to judging the cause of death.", "PMID": 970229}
{"id": "pubmed23n0032_7863", "title": "Five-year mortality in the city of Bergen, Norway, according to age, sex and blood pressure.", "content": "The City of Bergen was covered by a Mass Miniature Radiology Survey in 1963-64. On the initiative of the University of Bergen, examinations of BP were included. The initial survey has been reported previously (1). This analysis concerns the relationship between the 5 1/4-year cause-specific mortality and BP. Non-attenders have excess mortality in relation to attenders and this is mostly explained by a generally high mortality among bedridden people. The age-specific total mortality shows a clear pattern of a general increase with increasing BP. At high systolic BP levels, the 5-year mortality is independent of whether the age is 45 or 75. The systolic age-adjusted curve for males increases quite linearly, while the diastolic curve is more U-shaped. Thus, when comparing the predictive power of BP, allowance must be made for this fact. Using a second order polynomial prediction function, this conclusion is reversed. The mortality from cerebral stroke shows a dramatic increase with increasing BP. The diastolic curve shows a bend-off for high values, above 110 mmHg. This may be due to the offer of treatment which such patients received after the screening. Also the CHD mortality curve flattens for high BP values, especially for diastolic BP.", "contents": "Five-year mortality in the city of Bergen, Norway, according to age, sex and blood pressure. The City of Bergen was covered by a Mass Miniature Radiology Survey in 1963-64. On the initiative of the University of Bergen, examinations of BP were included. The initial survey has been reported previously (1). This analysis concerns the relationship between the 5 1/4-year cause-specific mortality and BP. Non-attenders have excess mortality in relation to attenders and this is mostly explained by a generally high mortality among bedridden people. The age-specific total mortality shows a clear pattern of a general increase with increasing BP. At high systolic BP levels, the 5-year mortality is independent of whether the age is 45 or 75. The systolic age-adjusted curve for males increases quite linearly, while the diastolic curve is more U-shaped. Thus, when comparing the predictive power of BP, allowance must be made for this fact. Using a second order polynomial prediction function, this conclusion is reversed. The mortality from cerebral stroke shows a dramatic increase with increasing BP. The diastolic curve shows a bend-off for high values, above 110 mmHg. This may be due to the offer of treatment which such patients received after the screening. Also the CHD mortality curve flattens for high BP values, especially for diastolic BP.", "PMID": 970230}
{"id": "pubmed23n0032_7864", "title": "A five-year survey of human Yersinia enterocolitica infections in Hungary.", "content": "Human infections associated with Yersinia enterocolitica in Hungary in the years 1969-1974 have been surveyed. During this period the public health laboratory network isolated 1355 strains from 1096 persons. The number of isolates according to sero-groups was: 1343 O3, 6 O9, 2 O1, 2a, 3 and 1 strain for O5, O6, O1O and O15 each. (ii) A total of 2192 serum specimens from patients gave positive agglutination reaction with antigen O3 in 26.8%, with antigen O9 in 3.2%. (iii) Fifty-six per cent of bacteriologically positive persons had enteritis. Other clinical forms (pseudoappendicitis and other abdominal complaints, erythema nodosum, rheumatoid arthritis) were encountered in O 1-2.7%. Symptomless excreters of Y. enterocolitica amounted to 23.1% of all positive persons. (iv) Patients with enteritis and symptomless excreters were rather evenly distributed between 10 and 60 years of age; 1--9-year-old children were affected more frequently (47.7% of all positive persons). Six distribution was, males: females = 1.5 : 1. In seasonal incidence yersiniosis differed from other enteric diseases: it showed a peak in the autumn-winter months. Sporadic cases and family outbreaks were the most frequent; epidemic infections in nurseries were also recorded. (v) Out of 59 animal strains 39 group O3 cultures were isolated from pigs, which may be assumed as the most important reservoir of yersinosis in Hungary.", "contents": "A five-year survey of human Yersinia enterocolitica infections in Hungary. Human infections associated with Yersinia enterocolitica in Hungary in the years 1969-1974 have been surveyed. During this period the public health laboratory network isolated 1355 strains from 1096 persons. The number of isolates according to sero-groups was: 1343 O3, 6 O9, 2 O1, 2a, 3 and 1 strain for O5, O6, O1O and O15 each. (ii) A total of 2192 serum specimens from patients gave positive agglutination reaction with antigen O3 in 26.8%, with antigen O9 in 3.2%. (iii) Fifty-six per cent of bacteriologically positive persons had enteritis. Other clinical forms (pseudoappendicitis and other abdominal complaints, erythema nodosum, rheumatoid arthritis) were encountered in O 1-2.7%. Symptomless excreters of Y. enterocolitica amounted to 23.1% of all positive persons. (iv) Patients with enteritis and symptomless excreters were rather evenly distributed between 10 and 60 years of age; 1--9-year-old children were affected more frequently (47.7% of all positive persons). Six distribution was, males: females = 1.5 : 1. In seasonal incidence yersiniosis differed from other enteric diseases: it showed a peak in the autumn-winter months. Sporadic cases and family outbreaks were the most frequent; epidemic infections in nurseries were also recorded. (v) Out of 59 animal strains 39 group O3 cultures were isolated from pigs, which may be assumed as the most important reservoir of yersinosis in Hungary.", "PMID": 970232}
{"id": "pubmed23n0032_7865", "title": "[Incidence of staphylococcal serotypes in different hospitals (author's transl)].", "content": "A total of 409 Staphylococcus aureus strains were classified with 17 typing sera into 16 groups and 14 types. Strains with the formula a b+ c e and h+ i k l were most frequently met with. The majority of strains isolated from typical staphylococcal diseases contained antigens e', h+ and/or h++ whereas those associated with atypical infections showed antigens b++ and o most frequently. Classification by Oeding and Williams' scheme yielded a less wide variety of serological units: most strains fell into group 4; spontaneously agglutinating and group 1, 2 and 3 strains were next in order.", "contents": "[Incidence of staphylococcal serotypes in different hospitals (author's transl)]. A total of 409 Staphylococcus aureus strains were classified with 17 typing sera into 16 groups and 14 types. Strains with the formula a b+ c e and h+ i k l were most frequently met with. The majority of strains isolated from typical staphylococcal diseases contained antigens e', h+ and/or h++ whereas those associated with atypical infections showed antigens b++ and o most frequently. Classification by Oeding and Williams' scheme yielded a less wide variety of serological units: most strains fell into group 4; spontaneously agglutinating and group 1, 2 and 3 strains were next in order.", "PMID": 970233}
{"id": "pubmed23n0032_7866", "title": "Suppression and disinhibition of instrumental alimentary reactions after successive lesions of the dorsomedial and lateral amygdala in dogs.", "content": "Damage of the dorsomedial amygdala produced impairment of instrumental performance to CS(+) with no changes to CS(-). Subsequent lesion of the lateral amygdala resulted in restoration of instrumental performance to CS(+) and transient disinhibition to CS(-). These results support the hypothesis that the dorsomedial amygdala is involved in facilitation, while the lateral amygdala in inhibition of alimmentary reactions.", "contents": "Suppression and disinhibition of instrumental alimentary reactions after successive lesions of the dorsomedial and lateral amygdala in dogs. Damage of the dorsomedial amygdala produced impairment of instrumental performance to CS(+) with no changes to CS(-). Subsequent lesion of the lateral amygdala resulted in restoration of instrumental performance to CS(+) and transient disinhibition to CS(-). These results support the hypothesis that the dorsomedial amygdala is involved in facilitation, while the lateral amygdala in inhibition of alimmentary reactions.", "PMID": 970234}
{"id": "pubmed23n0032_7867", "title": "Hyperthermia and inhibition of feeding produced by self-stimulation in the septum and preoptic area in dogs.", "content": "Dogs bearing electrodes implanted in the anterior part of the basal forebrain were tested for their response to food upon electric stimulation of rewarding sites, and for self-stimulation-produced hyperthermia. Self-stimulation and forced (experimenter-induced) stimulation of 12 out of 16 loci evoked a negative reaction to food the strength of which was determined according to occurrence and persistence of three effects: ignoring food (the dog performing self-stimulation in the presence of readily available meat), food rejection (the dog's failure to take meat offered together with passive stimulation of the rewarding site), and food ejection (throwing meat out of the mouth upon passive stimulation). The rise in body temperature during self-stimulation was positively correlated with the rate of responding. Hyperthermia was significantly higher during self-stimulation in sites where stimulation produced a strong negative reaction to food, as compared with those where stimulation failed to stop the animal from eating. The stimulus-contingent negative reaction to food may reflect a short-term satiety which is supposed to play an essential role in the mechanism of reinforcement. Hyperthermia, and particularly stabilization of hypothalamic temperature on an elevated but fairly constant level, argues for a shift of the set-point for temperature regulation. Occurrence of the two effects supports the claim that self-stimulation produces some complex activation of neural processes controlling energy homeostasis.", "contents": "Hyperthermia and inhibition of feeding produced by self-stimulation in the septum and preoptic area in dogs. Dogs bearing electrodes implanted in the anterior part of the basal forebrain were tested for their response to food upon electric stimulation of rewarding sites, and for self-stimulation-produced hyperthermia. Self-stimulation and forced (experimenter-induced) stimulation of 12 out of 16 loci evoked a negative reaction to food the strength of which was determined according to occurrence and persistence of three effects: ignoring food (the dog performing self-stimulation in the presence of readily available meat), food rejection (the dog's failure to take meat offered together with passive stimulation of the rewarding site), and food ejection (throwing meat out of the mouth upon passive stimulation). The rise in body temperature during self-stimulation was positively correlated with the rate of responding. Hyperthermia was significantly higher during self-stimulation in sites where stimulation produced a strong negative reaction to food, as compared with those where stimulation failed to stop the animal from eating. The stimulus-contingent negative reaction to food may reflect a short-term satiety which is supposed to play an essential role in the mechanism of reinforcement. Hyperthermia, and particularly stabilization of hypothalamic temperature on an elevated but fairly constant level, argues for a shift of the set-point for temperature regulation. Occurrence of the two effects supports the claim that self-stimulation produces some complex activation of neural processes controlling energy homeostasis.", "PMID": 970235}
{"id": "pubmed23n0032_7868", "title": "Learning, stress, and psychosomatic symptoms.", "content": "Professor Jerzy Konorski was an internationally renowned scientist with a remarkable range of intellectual power and enthusiasm; he also was a warm friend. It is a great honor to be invited to contribute a paper to publications commemorating his life and work. As infectious diseases are being conquered, other pathological conditions are assuming increasing medical importance. A number of these are strongly influenced by the brain in its central role of regulating a wide variety of vital functions. It has long been believed that the mind affects the body; recent research in supplying objective evidence for an increasing number of such psychosomatic effects. This paper summarizes some experiments from the author's Laboratory on such effects and also on some of the ways in which amines and hormones in the brain affect behavior. In view of the wide range of Professor Konorski's interests, it is fitting that the work to be summarized here has been multidisciplinary. Clinical studies, especially under conditions of combat in war, show that fear and the conflict it can induce can produce a wide range of psychosomatic, neurotic, and even psychotic symptoms. Such work also indicates that purely psychological factors, such as learning when it is dangerous and when it is safe and learning coping responses, can make a great difference in the amount of fear experienced and in its psychosomatic effects. However, while clinical observations of human behavior are particularly relevant, it is difficult to untangle confounding factors and thus determine unequivocal causal relationships. The immediately following experiments attempt to fill part of this gap.", "contents": "Learning, stress, and psychosomatic symptoms. Professor Jerzy Konorski was an internationally renowned scientist with a remarkable range of intellectual power and enthusiasm; he also was a warm friend. It is a great honor to be invited to contribute a paper to publications commemorating his life and work. As infectious diseases are being conquered, other pathological conditions are assuming increasing medical importance. A number of these are strongly influenced by the brain in its central role of regulating a wide variety of vital functions. It has long been believed that the mind affects the body; recent research in supplying objective evidence for an increasing number of such psychosomatic effects. This paper summarizes some experiments from the author's Laboratory on such effects and also on some of the ways in which amines and hormones in the brain affect behavior. In view of the wide range of Professor Konorski's interests, it is fitting that the work to be summarized here has been multidisciplinary. Clinical studies, especially under conditions of combat in war, show that fear and the conflict it can induce can produce a wide range of psychosomatic, neurotic, and even psychotic symptoms. Such work also indicates that purely psychological factors, such as learning when it is dangerous and when it is safe and learning coping responses, can make a great difference in the amount of fear experienced and in its psychosomatic effects. However, while clinical observations of human behavior are particularly relevant, it is difficult to untangle confounding factors and thus determine unequivocal causal relationships. The immediately following experiments attempt to fill part of this gap.", "PMID": 970236}
{"id": "pubmed23n0032_7869", "title": "Visual cortex role in object discrimination in cats deprived of pattern vision from birth.", "content": "A simultaneous object discrimination task for food reward was elaborated in normal cats and in cats deprived of pattern vision from birth. In the latter the learning was more difficult and retraining was necessary after a preoperative retention period. In both groups the cortical projection of central vision was removed in areas 17, 18 and 19. In deprived cats the deficit following the cortical lesion was less than in normal animals. It is concluded that deprived cats used visual cortex less in learning and thus they learned more slowly but were less deficient postoperatively.", "contents": "Visual cortex role in object discrimination in cats deprived of pattern vision from birth. A simultaneous object discrimination task for food reward was elaborated in normal cats and in cats deprived of pattern vision from birth. In the latter the learning was more difficult and retraining was necessary after a preoperative retention period. In both groups the cortical projection of central vision was removed in areas 17, 18 and 19. In deprived cats the deficit following the cortical lesion was less than in normal animals. It is concluded that deprived cats used visual cortex less in learning and thus they learned more slowly but were less deficient postoperatively.", "PMID": 970237}
{"id": "pubmed23n0032_7870", "title": "EEG correlates of acquisition and extinction of expectancy.", "content": "The work to be described was begun in 1958 at the Montreal Neurological Institute in order to verify some of the prevailing neurophysiological notions concerning the mechanisms of habituation and attention. The results dealing with the EEG correlates of habituation and acquisition of expectancy were presented at the annual meetings of the American Psychological Association (Mahut 1959). Beginning in 1960 other authors started questioning the validity of the claims regarding mechanisms of attention that had been previously reported in the physiological literature. I hesitated, therefore, to publish my data. Professor Konorski, however, kindly urged me to do so, particularly the results concerned with the EEG of extinction and the effects of arousal on cortical electrophysiology. It is to his memory that this report is dedicated.", "contents": "EEG correlates of acquisition and extinction of expectancy. The work to be described was begun in 1958 at the Montreal Neurological Institute in order to verify some of the prevailing neurophysiological notions concerning the mechanisms of habituation and attention. The results dealing with the EEG correlates of habituation and acquisition of expectancy were presented at the annual meetings of the American Psychological Association (Mahut 1959). Beginning in 1960 other authors started questioning the validity of the claims regarding mechanisms of attention that had been previously reported in the physiological literature. I hesitated, therefore, to publish my data. Professor Konorski, however, kindly urged me to do so, particularly the results concerned with the EEG of extinction and the effects of arousal on cortical electrophysiology. It is to his memory that this report is dedicated.", "PMID": 970239}
{"id": "pubmed23n0032_7871", "title": "The effect of conditioned stimuli signalling food upon the autochthonous instrumental responses in dogs.", "content": "Food CSs, presented (i) concomitantly with SD controlling bar pressing for food, or (ii) on the background of non-discriminated bar pressing (FR 1/15), suppress instrumental performance but elicit undiminished conditioned salivation. This result supports Soltysik-Konorski's model of CNS mechanism controlling food-oriented behavior which postulates drive inhibition by taste-consummatory neurons.", "contents": "The effect of conditioned stimuli signalling food upon the autochthonous instrumental responses in dogs. Food CSs, presented (i) concomitantly with SD controlling bar pressing for food, or (ii) on the background of non-discriminated bar pressing (FR 1/15), suppress instrumental performance but elicit undiminished conditioned salivation. This result supports Soltysik-Konorski's model of CNS mechanism controlling food-oriented behavior which postulates drive inhibition by taste-consummatory neurons.", "PMID": 970240}
{"id": "pubmed23n0032_7872", "title": "Discrimination of time intervals in cats.", "content": "Fourteen cats were trained to discriminate between 5 and 20 s periods of confinement as evidenced by differential responding to two feeders. In a subsequent titration procedure the cats discriminated 5 from 10 or even 8 s. Positional mediation of correct responses was observed only in some animals. The present task may complement the classical and operant conditioning situations in which the temporal distribution of responses reflects the animals' abilities for both time discrimination and response inhibition.", "contents": "Discrimination of time intervals in cats. Fourteen cats were trained to discriminate between 5 and 20 s periods of confinement as evidenced by differential responding to two feeders. In a subsequent titration procedure the cats discriminated 5 from 10 or even 8 s. Positional mediation of correct responses was observed only in some animals. The present task may complement the classical and operant conditioning situations in which the temporal distribution of responses reflects the animals' abilities for both time discrimination and response inhibition.", "PMID": 970241}
{"id": "pubmed23n0032_7873", "title": "Efferent connections of the basolateral amygdaloid part to the archi-, paleo-, and neocortex in dogs.", "content": "Small electrolytic lesions were placed in the basal and lateral amygdaloid nuclei of the dog and the distribution of degenerating fibers was studied with Nauta and Fink-Heimer modifications of the impregnation methods. Degenerating axons were followed into the hippocampal region and entorhinal cortex as well as insular and temporal cortices. The present results suggest that: (i) The hippocampal region receives projections from the basal parvocellular and basal magnocellular nuclei; (ii) the entorhinal cortex from the lateral and basal parvocellular nuclei; (iii) the insular cortex, cortex of the anterior ectosylvian and sylvian gyri, and to a smaller degree the claustrum, from the lateral and basal magnocellular nuclei; (iv) the temporal cortex of the posterior suprasylvian gyrus from all nuclei of the basolateral part of the amygdala. It is concluded that the basolateral part of the amygdala in the dog has a complex and highly developed connections with the archi-, paleo- and neocortex.", "contents": "Efferent connections of the basolateral amygdaloid part to the archi-, paleo-, and neocortex in dogs. Small electrolytic lesions were placed in the basal and lateral amygdaloid nuclei of the dog and the distribution of degenerating fibers was studied with Nauta and Fink-Heimer modifications of the impregnation methods. Degenerating axons were followed into the hippocampal region and entorhinal cortex as well as insular and temporal cortices. The present results suggest that: (i) The hippocampal region receives projections from the basal parvocellular and basal magnocellular nuclei; (ii) the entorhinal cortex from the lateral and basal parvocellular nuclei; (iii) the insular cortex, cortex of the anterior ectosylvian and sylvian gyri, and to a smaller degree the claustrum, from the lateral and basal magnocellular nuclei; (iv) the temporal cortex of the posterior suprasylvian gyrus from all nuclei of the basolateral part of the amygdala. It is concluded that the basolateral part of the amygdala in the dog has a complex and highly developed connections with the archi-, paleo- and neocortex.", "PMID": 970242}
{"id": "pubmed23n0032_7874", "title": "Localization of acetylcholinesterase activity in the amygdaloid body of man.", "content": "The localization of acetylcholinesterase (AChE) activity in the nuclei of the amygdaloid body of man was studied by Gerebtzoff's modification of Koelle's acetylthiocholine method on 10 human brains. To compare the histochemical results with the cytoarchitectionics, additionally sections from 10 human brains were stained with cresyl violet. The intensity of AChE activity in the different amygdaloid nuclei varied widely. The high AChE activity occurred in the basal lateral nucleus and in the lateral part of the central nucleus. Very little activity was found in the medial nucleus and in the intermediate part of the central nucleus. All remaining nuclei showed AChE activity of moderate intensity. In almost each amygdaloid nucleus, differences of AChE activity among various areas were observed. In the amygdala of man all nuclei described in animals were found except nucleus of the lateral olfactory tract. Most of the human amygdaloid nuclei show more differentiated structure than in lower mammals in terms of not only cytoarchitectonic subdivision, but also in the intensity of AChE activity.", "contents": "Localization of acetylcholinesterase activity in the amygdaloid body of man. The localization of acetylcholinesterase (AChE) activity in the nuclei of the amygdaloid body of man was studied by Gerebtzoff's modification of Koelle's acetylthiocholine method on 10 human brains. To compare the histochemical results with the cytoarchitectionics, additionally sections from 10 human brains were stained with cresyl violet. The intensity of AChE activity in the different amygdaloid nuclei varied widely. The high AChE activity occurred in the basal lateral nucleus and in the lateral part of the central nucleus. Very little activity was found in the medial nucleus and in the intermediate part of the central nucleus. All remaining nuclei showed AChE activity of moderate intensity. In almost each amygdaloid nucleus, differences of AChE activity among various areas were observed. In the amygdala of man all nuclei described in animals were found except nucleus of the lateral olfactory tract. Most of the human amygdaloid nuclei show more differentiated structure than in lower mammals in terms of not only cytoarchitectonic subdivision, but also in the intensity of AChE activity.", "PMID": 970243}
{"id": "pubmed23n0032_7875", "title": "The effects of cerebral sensory cortical ablation on instrumental conditioned reflex in cats with cerebellar lesions.", "content": "Cats were trained to reach with their fore limbs for food placed in horizontally mounted cylinders of various diameters. The chain instrumental reflex (entering cylinder, reaching for, grasping and bringing food toward the mouth) survived cerebellar paravermal cortical ablation or interposite-dentate nuclear lesion, with some exaggerated flexor or extensor responses respectively. Movements improved with time. Cats were then subjected to ablation of cerebral sensory cortex, SI and SII. SI or compined SI and SII lesion resulted in an initial period of decompensation of cerebellar symptoms. Sensory loss was also noted for 30-40 days after SI lesion and throughout the observation period up to 53 days after combined SI and SII lesion. The conditioned responses were soon accomplished at preoperative level. Furthermore, visual occlusion did not change the performance in conditioning situation. The result indicates that the compensation of cerebellar symptoms does not depend on an intact cerebral sensory cortex. It also suggests that functional accomplishment can be obtained through training despite neurological deficits following certain brain damage.", "contents": "The effects of cerebral sensory cortical ablation on instrumental conditioned reflex in cats with cerebellar lesions. Cats were trained to reach with their fore limbs for food placed in horizontally mounted cylinders of various diameters. The chain instrumental reflex (entering cylinder, reaching for, grasping and bringing food toward the mouth) survived cerebellar paravermal cortical ablation or interposite-dentate nuclear lesion, with some exaggerated flexor or extensor responses respectively. Movements improved with time. Cats were then subjected to ablation of cerebral sensory cortex, SI and SII. SI or compined SI and SII lesion resulted in an initial period of decompensation of cerebellar symptoms. Sensory loss was also noted for 30-40 days after SI lesion and throughout the observation period up to 53 days after combined SI and SII lesion. The conditioned responses were soon accomplished at preoperative level. Furthermore, visual occlusion did not change the performance in conditioning situation. The result indicates that the compensation of cerebellar symptoms does not depend on an intact cerebral sensory cortex. It also suggests that functional accomplishment can be obtained through training despite neurological deficits following certain brain damage.", "PMID": 970244}
{"id": "pubmed23n0032_7876", "title": "The orienting reflex in cats with experimental temporal lobe epilepsy.", "content": "Aluminum-induced temporal lobe epilepsy in 24 cats showed marked interictal intensification of somatic and EEG components of the orienting reaction and increase in their resistance to habituation. The greatest disturbances of the orienting response were noted in cases of bitemporal foci, of foci situated in the allocortex (hippocampus and amygdala) and of foci inducing generalized or generalized and partial seizures.", "contents": "The orienting reflex in cats with experimental temporal lobe epilepsy. Aluminum-induced temporal lobe epilepsy in 24 cats showed marked interictal intensification of somatic and EEG components of the orienting reaction and increase in their resistance to habituation. The greatest disturbances of the orienting response were noted in cases of bitemporal foci, of foci situated in the allocortex (hippocampus and amygdala) and of foci inducing generalized or generalized and partial seizures.", "PMID": 970245}
{"id": "pubmed23n0032_7877", "title": "The effect of leukocyte-induced degradation products of albumin on the analgesic action of morphine.", "content": "The effect of leukocyte-induced human albumin degradation products (LPAD) on the analgesic potency of morphine was investigated. The degradation products given alone to the rats did not affect pain threshold; while combined with morphine, they potentiated its analgesic action. Moreover, the degradation products increased the concentration of morphine in the brain tissue.", "contents": "The effect of leukocyte-induced degradation products of albumin on the analgesic action of morphine. The effect of leukocyte-induced human albumin degradation products (LPAD) on the analgesic potency of morphine was investigated. The degradation products given alone to the rats did not affect pain threshold; while combined with morphine, they potentiated its analgesic action. Moreover, the degradation products increased the concentration of morphine in the brain tissue.", "PMID": 970246}
{"id": "pubmed23n0032_7878", "title": "Activity of some enzymes in the cortex of the cat with pretrigeminal brainstem transection.", "content": "The visual and somatic sensory cortex were investigated. The activity of acetylcholinesterase and lactate dehydrogenase was similar in the pretrigeminal and non-operated cats. The activity of these two enzymes, as well as that of choline acetyltransferase, butyrylcholinesterase and acid phosphatase was similar 3 and 8 h after the pretrigeminal transection. Results support the possibility of utilizing the pretrigeminal cat for investigations of biochemical correlates of learning.", "contents": "Activity of some enzymes in the cortex of the cat with pretrigeminal brainstem transection. The visual and somatic sensory cortex were investigated. The activity of acetylcholinesterase and lactate dehydrogenase was similar in the pretrigeminal and non-operated cats. The activity of these two enzymes, as well as that of choline acetyltransferase, butyrylcholinesterase and acid phosphatase was similar 3 and 8 h after the pretrigeminal transection. Results support the possibility of utilizing the pretrigeminal cat for investigations of biochemical correlates of learning.", "PMID": 970247}
{"id": "pubmed23n0032_7879", "title": "[Diagnosis and differential diagnosis of solitary pulmonary coin lesions].", "content": "In the present analysis, the diagnosis as well as the differential diagnosis of solitary pulmonary coin lesions are discussed. The coin lesions of 361 patients who were at our diagnostic ward from 1968 to 1970 were defined according to Rube [1967]. The investigations are in accordance with the routine examinations and were consciously demonstrated in nonselected patients in order to express the several diagnostic procedures, their field of indication and their efficiency in a balanced ratio. Therefore, the efficacy of the X-ray diagnosis could be proved in a broader range than in a collective of circular foci which would have been chosen exclusively under the aspect of the diagnosis by means of needle biopsy and which would reveal a higher percentage of morphological clarification of the focus.", "contents": "[Diagnosis and differential diagnosis of solitary pulmonary coin lesions]. In the present analysis, the diagnosis as well as the differential diagnosis of solitary pulmonary coin lesions are discussed. The coin lesions of 361 patients who were at our diagnostic ward from 1968 to 1970 were defined according to Rube [1967]. The investigations are in accordance with the routine examinations and were consciously demonstrated in nonselected patients in order to express the several diagnostic procedures, their field of indication and their efficiency in a balanced ratio. Therefore, the efficacy of the X-ray diagnosis could be proved in a broader range than in a collective of circular foci which would have been chosen exclusively under the aspect of the diagnosis by means of needle biopsy and which would reveal a higher percentage of morphological clarification of the focus.", "PMID": 970277}
{"id": "pubmed23n0032_7880", "title": "BCG vaccination and epidemiological situation: a decision making approach to the use of BCG.", "content": "The clinical efficacy of a well-controlled and correctly applied BCG vaccine can hardly be questioned, nor its usefulness under individual circumstances. But a problem more and more often raised now is that of the opportuneness, at the present time, to still use BCG on a mass- systematic - scale, particularly in the TB control programmes of low-prevalence countries. The article proposes an approach which, alongside with other methods already presented for assessing the epidemiological value of BCG and its public health indications, can help appreciate the advantages and disadvantages brought by vaccination in high- as well as in low-prevalence countries. The article focuses on the future, i.e. on what can, from now on, be expected from BCG in terms of epidemiological, economical and psychological benefits. Test runs were made considering three cohorts of 100,000 individuals (newborn, school enterers, school leavers), vaccinated or not vaccinated, submitted to various risks of infection (corresponding to conditions of high-, intermediate- and low-prevalence of tuberculosis) and experiencing various breakdown rates from infection to disease. Cost for BCG on the one hand, and cost for diagnosis, treatment of tuberculosis and loss of wages on the other, were also entered in the calculations; an attempt was also made to quantify in absolute terms and in relative ones the in conveniences linked to the application of BCG itself. Beyond figures and results presented as examples, what is more important is that the article furnished a method of reasoning and the possibility to feed one's own data in the model; it may help to encompass the scope and implications of a decision, and it furnishes elements of dialogue with other members of the planning team. It should be well realized that the elements provided and those which can be worked out are elements of reflexion to found a decision; the decision itself - to start, to pursue or to stop BCG on a mass scale - is of political nature (public health policy): it always implies a value judgement - from the part of the providers of the services and/or, preferably, from the part of the users, i.e. a preference which is the result of a 'weighing' between advantages and inevitable disadvantages, both involving time, place, existing system, existing beliefs and other fields of health and welfare.", "contents": "BCG vaccination and epidemiological situation: a decision making approach to the use of BCG. The clinical efficacy of a well-controlled and correctly applied BCG vaccine can hardly be questioned, nor its usefulness under individual circumstances. But a problem more and more often raised now is that of the opportuneness, at the present time, to still use BCG on a mass- systematic - scale, particularly in the TB control programmes of low-prevalence countries. The article proposes an approach which, alongside with other methods already presented for assessing the epidemiological value of BCG and its public health indications, can help appreciate the advantages and disadvantages brought by vaccination in high- as well as in low-prevalence countries. The article focuses on the future, i.e. on what can, from now on, be expected from BCG in terms of epidemiological, economical and psychological benefits. Test runs were made considering three cohorts of 100,000 individuals (newborn, school enterers, school leavers), vaccinated or not vaccinated, submitted to various risks of infection (corresponding to conditions of high-, intermediate- and low-prevalence of tuberculosis) and experiencing various breakdown rates from infection to disease. Cost for BCG on the one hand, and cost for diagnosis, treatment of tuberculosis and loss of wages on the other, were also entered in the calculations; an attempt was also made to quantify in absolute terms and in relative ones the in conveniences linked to the application of BCG itself. Beyond figures and results presented as examples, what is more important is that the article furnished a method of reasoning and the possibility to feed one's own data in the model; it may help to encompass the scope and implications of a decision, and it furnishes elements of dialogue with other members of the planning team. It should be well realized that the elements provided and those which can be worked out are elements of reflexion to found a decision; the decision itself - to start, to pursue or to stop BCG on a mass scale - is of political nature (public health policy): it always implies a value judgement - from the part of the providers of the services and/or, preferably, from the part of the users, i.e. a preference which is the result of a 'weighing' between advantages and inevitable disadvantages, both involving time, place, existing system, existing beliefs and other fields of health and welfare.", "PMID": 970278}
{"id": "pubmed23n0032_7881", "title": "Rapid purification of human high molecular weight kininogen.", "content": "Human high molecular weight kininogen was isolated by a rapid procedure, using anion exchange chromatography on QAE-Sephadex, ammonium sulfate precipitation and cation exchange chromatography on CM-Sephadex. The poor recovery and relatively low specific activity observed in earlier experiments was found to be due to a contaminant, presumably enzymatic, capable of releasing kinin from the kininogen. The \"spontaneous\" kinin release was blocked by soy bean trypsin inhibitor and by C1-inactivator. The isolated kininogen was stable at different temperatures, did not contain free kinin and was a good substrate for plasma kallikrein and plasmin.", "contents": "Rapid purification of human high molecular weight kininogen. Human high molecular weight kininogen was isolated by a rapid procedure, using anion exchange chromatography on QAE-Sephadex, ammonium sulfate precipitation and cation exchange chromatography on CM-Sephadex. The poor recovery and relatively low specific activity observed in earlier experiments was found to be due to a contaminant, presumably enzymatic, capable of releasing kinin from the kininogen. The \"spontaneous\" kinin release was blocked by soy bean trypsin inhibitor and by C1-inactivator. The isolated kininogen was stable at different temperatures, did not contain free kinin and was a good substrate for plasma kallikrein and plasmin.", "PMID": 970286}
{"id": "pubmed23n0032_7882", "title": "Diamines and polyamines in DMBA-induced breast carcinoma containing mast cells resistant to compound 48/80.", "content": "In female rats, mammary carcinoma were induced by DMBA (7,12-dimethylbenz(alpha)anthracene) administration. The activities of histidine and ornithine decarboxylases and the concentrations of histamine, putrescine and polyamines were determined in tumour extracts and urine. In the tumour tissue, formation of histamine and putrescine took place at much higher rates than in normal mammary tissue. Administration of compound 48/80 in doses that mobilized about 70% of histamine contained in the ear failed to release histamine contained in the tumour; 48/80 also failed to produce histological signs of degranulation of the tumour mast cells. It thus appears that these cells are different from mast cells in most normal tissues, a distinction that calls for further study.", "contents": "Diamines and polyamines in DMBA-induced breast carcinoma containing mast cells resistant to compound 48/80. In female rats, mammary carcinoma were induced by DMBA (7,12-dimethylbenz(alpha)anthracene) administration. The activities of histidine and ornithine decarboxylases and the concentrations of histamine, putrescine and polyamines were determined in tumour extracts and urine. In the tumour tissue, formation of histamine and putrescine took place at much higher rates than in normal mammary tissue. Administration of compound 48/80 in doses that mobilized about 70% of histamine contained in the ear failed to release histamine contained in the tumour; 48/80 also failed to produce histological signs of degranulation of the tumour mast cells. It thus appears that these cells are different from mast cells in most normal tissues, a distinction that calls for further study.", "PMID": 970287}
{"id": "pubmed23n0032_7883", "title": "Comparison of the immunosuppressive efficacy of 6-mercaptopurine, azathioprine, cyclophosphamide and 036.5122 (Asta) on the primary and secondary immune response of mice to sheep erythrocytes.", "content": "Two alkylating (cyclophosphamide and 036.5122 Asta) and two antiproliferative agents (6-mercaptopurine and azathioprine) have been compared for their immuno-suppressive potency on the primary and secondary humoral immune response of mice. If equitoxic dosages of the respective drugs are compared, the alkylating agents proved to be of much higher immunosuppressive potency than the antiproliferative agents. In non toxic dosages alkylating agents were able to completely inhibit a primary or secondary immune response, whilst a similar effect with antiproliferative drugs could not be obtained even within toxic dose ranges. Induction of immunological tolerance was possible only by use of the alkylating agents. The significance of these comparative studies is discussed in view of the frequent use of the tested drugs in clinical immunosuppression.", "contents": "Comparison of the immunosuppressive efficacy of 6-mercaptopurine, azathioprine, cyclophosphamide and 036.5122 (Asta) on the primary and secondary immune response of mice to sheep erythrocytes. Two alkylating (cyclophosphamide and 036.5122 Asta) and two antiproliferative agents (6-mercaptopurine and azathioprine) have been compared for their immuno-suppressive potency on the primary and secondary humoral immune response of mice. If equitoxic dosages of the respective drugs are compared, the alkylating agents proved to be of much higher immunosuppressive potency than the antiproliferative agents. In non toxic dosages alkylating agents were able to completely inhibit a primary or secondary immune response, whilst a similar effect with antiproliferative drugs could not be obtained even within toxic dose ranges. Induction of immunological tolerance was possible only by use of the alkylating agents. The significance of these comparative studies is discussed in view of the frequent use of the tested drugs in clinical immunosuppression.", "PMID": 970288}
{"id": "pubmed23n0032_7884", "title": "Thrombus formation, hemostasis, adhesiveness of leukocytes and morphological abnormalities in the microcirculation of adjuvant arthritic rats.", "content": "Microcirculatory alterations which may be responsible for the chronicity of adjuvant-induced arthritis in the rat were investigated. Thrombus formation in the mesenteric microcirculation was investigated by laser-technique. In arthritic rats thrombus formation for a given stimulus was increased significantly and a correlation was found between the intensity of the arthritic changes and this thrombotic reaction. Adherence of leukocytes to the venous endothelium was studied by an in vivo technique. The number of \"rolling\" and \"sticking\" leukocytes was greatly increased in the mesenteric venules of arthritic rats. Acute treatment of severely arthritic rats with aspirin greatly reduced the increased adhesiveness of granulocytes. By the use of an atraumatic technique for vital microscopy of the microcirculation of skin of arthritic rats, striking morphologic changes with stasis of erythrocytes were observed. Morphological aberrations were also found in the mesenteric microvessels of diseased animals.", "contents": "Thrombus formation, hemostasis, adhesiveness of leukocytes and morphological abnormalities in the microcirculation of adjuvant arthritic rats. Microcirculatory alterations which may be responsible for the chronicity of adjuvant-induced arthritis in the rat were investigated. Thrombus formation in the mesenteric microcirculation was investigated by laser-technique. In arthritic rats thrombus formation for a given stimulus was increased significantly and a correlation was found between the intensity of the arthritic changes and this thrombotic reaction. Adherence of leukocytes to the venous endothelium was studied by an in vivo technique. The number of \"rolling\" and \"sticking\" leukocytes was greatly increased in the mesenteric venules of arthritic rats. Acute treatment of severely arthritic rats with aspirin greatly reduced the increased adhesiveness of granulocytes. By the use of an atraumatic technique for vital microscopy of the microcirculation of skin of arthritic rats, striking morphologic changes with stasis of erythrocytes were observed. Morphological aberrations were also found in the mesenteric microvessels of diseased animals.", "PMID": 970289}
{"id": "pubmed23n0032_7885", "title": "Pertussis vaccine oedema: an experimental model for the action of penicillamine-like drugs.", "content": "A delayed hypersensitivity response was induced in the rat paw using pertussis vaccine. Oedema was measured after the challenging injection. D-penicillamine and levamisole enhanced the response, while indomethacin suppressed it. This model is useful to distinguish the effects of anti-inflammatory drugs from those like D-Penicillamine which have a specific activity in rheumatoid arthritis.", "contents": "Pertussis vaccine oedema: an experimental model for the action of penicillamine-like drugs. A delayed hypersensitivity response was induced in the rat paw using pertussis vaccine. Oedema was measured after the challenging injection. D-penicillamine and levamisole enhanced the response, while indomethacin suppressed it. This model is useful to distinguish the effects of anti-inflammatory drugs from those like D-Penicillamine which have a specific activity in rheumatoid arthritis.", "PMID": 970290}
{"id": "pubmed23n0032_7886", "title": "Pertussis vaccine pleurisy: a model of delayed hypersensitivity.", "content": "Male Wistar rats have been sensitized to Bordetella pertussis using a mixture of Freund's incomplete adjuvant and pertussis organisms. Intrapleural challenge 12 days later with pertussis produced a marked delayed inflammatory response, maximal at 48 hours and dominated by influx of mononuclear cells. Dosing with D-penicillamine (25 mg/kg) and levamisole (5 mg/kg) at the time of challenge produced a significant enhancement of the reaction. A long period of dosing with either drug, or treatment with indomethacin (3 mg/kg), suppressed the response. The relevance of this to the testing and mode of action of antirheumatic drugs is discussed.", "contents": "Pertussis vaccine pleurisy: a model of delayed hypersensitivity. Male Wistar rats have been sensitized to Bordetella pertussis using a mixture of Freund's incomplete adjuvant and pertussis organisms. Intrapleural challenge 12 days later with pertussis produced a marked delayed inflammatory response, maximal at 48 hours and dominated by influx of mononuclear cells. Dosing with D-penicillamine (25 mg/kg) and levamisole (5 mg/kg) at the time of challenge produced a significant enhancement of the reaction. A long period of dosing with either drug, or treatment with indomethacin (3 mg/kg), suppressed the response. The relevance of this to the testing and mode of action of antirheumatic drugs is discussed.", "PMID": 970291}
{"id": "pubmed23n0032_7887", "title": "Inflammation and the vascular changes due to thermal injury in rat hind paws.", "content": "The vascular changes due to thermal injury have been determined in rat hind paws. In anaesthetized rats one hind paw has been injured by immersion in water for 30 seconds; temperature range 49-65 degrees C. Changes in blood flow, blood content and albumin content have been determined and compared with values from uninjured rats. The swelling caused by thermal injury was temperature-dependent, the higher temperatures caused the larger swellings. There were also temperature-dependent increases in blood flow to the injured paw and albumin content of the injured paw. Changes in blood content only occurred at the highest temperatures. Swelling, increased blood flow and albumin content could be detected within 1 minute of injury. The swelling and albumin content of the tissue then continued to increase gradually. The increase in blood flow was greatest 1 minute after injury and then stabilized at a flow some 4-5 times higher than normal paw flow. The method described can be used to measure vascular changes due to other inflammatory stimuli and could be applied to measure changes in other discreet areas.", "contents": "Inflammation and the vascular changes due to thermal injury in rat hind paws. The vascular changes due to thermal injury have been determined in rat hind paws. In anaesthetized rats one hind paw has been injured by immersion in water for 30 seconds; temperature range 49-65 degrees C. Changes in blood flow, blood content and albumin content have been determined and compared with values from uninjured rats. The swelling caused by thermal injury was temperature-dependent, the higher temperatures caused the larger swellings. There were also temperature-dependent increases in blood flow to the injured paw and albumin content of the injured paw. Changes in blood content only occurred at the highest temperatures. Swelling, increased blood flow and albumin content could be detected within 1 minute of injury. The swelling and albumin content of the tissue then continued to increase gradually. The increase in blood flow was greatest 1 minute after injury and then stabilized at a flow some 4-5 times higher than normal paw flow. The method described can be used to measure vascular changes due to other inflammatory stimuli and could be applied to measure changes in other discreet areas.", "PMID": 970292}
{"id": "pubmed23n0032_7888", "title": "Modification of adjuvant inflammation in rats deficient in essential fatty acids.", "content": "Rats deficient in essential fatty acids (EFA) did not develop as much foot swelling on receiving injections of complete Freund's adjuvant as did normal controls. Both the acute inflammation and the chronic inflammation were affected. This reduction in the chronic phase of adjuvant inflammation was restored to normal levels by feeding a small supplement of corn oil as a dietary source of EFA. Since the EFA are biologic precursors of prostaglandins (PG), the lack of EFA is thought to influence adjuvant-induced inflammation by reducing available PG mediators of inflammation.", "contents": "Modification of adjuvant inflammation in rats deficient in essential fatty acids. Rats deficient in essential fatty acids (EFA) did not develop as much foot swelling on receiving injections of complete Freund's adjuvant as did normal controls. Both the acute inflammation and the chronic inflammation were affected. This reduction in the chronic phase of adjuvant inflammation was restored to normal levels by feeding a small supplement of corn oil as a dietary source of EFA. Since the EFA are biologic precursors of prostaglandins (PG), the lack of EFA is thought to influence adjuvant-induced inflammation by reducing available PG mediators of inflammation.", "PMID": 970293}
{"id": "pubmed23n0032_7889", "title": "Release of prostaglandins from the isolated perfused rabbit ear by bradykinin and acetylcholine.", "content": "(1) The isolated rabbit ear was perfused via its artery and the venous outflow superfused a PGE-sensitive rat stomach strip or a PGF-sensitive rat colon. (2) Injection of bradykinin intra-arterially into the ear produced a larger contraction of the rat stomach strip than the application of the same dose of bradykinin directly to the superfused muscle. (3) This difference is explained as a release of PGE-like material by bradykinin since indomethacin (infused i.a. into the ear) reduced the effect of the i.a. applied bradykinin. (4) PGF-like material could not be detected in the venous effluent. (5) ACh released only minimal amounts of PGE-like substance. (6) CONCLUSION: The amount of PGE-like material released by bradykinin is large enough to sensitize the paravascular pain receptors in the rabbit ear for the attack of bradykinin. Therefore, inhibition of PG-synthesis (i.e. by indomethacin) or inhibition of the sensitizing action of E-type PGs (i.e. by polyphloretin phosphate) reduces the pain producing effect of bradykinin. Since ACh releases only minimal amounts of E-type PGs, its effect is reduced only to a minimal extent by indomethacin or polyphloretin phosphate.", "contents": "Release of prostaglandins from the isolated perfused rabbit ear by bradykinin and acetylcholine. (1) The isolated rabbit ear was perfused via its artery and the venous outflow superfused a PGE-sensitive rat stomach strip or a PGF-sensitive rat colon. (2) Injection of bradykinin intra-arterially into the ear produced a larger contraction of the rat stomach strip than the application of the same dose of bradykinin directly to the superfused muscle. (3) This difference is explained as a release of PGE-like material by bradykinin since indomethacin (infused i.a. into the ear) reduced the effect of the i.a. applied bradykinin. (4) PGF-like material could not be detected in the venous effluent. (5) ACh released only minimal amounts of PGE-like substance. (6) CONCLUSION: The amount of PGE-like material released by bradykinin is large enough to sensitize the paravascular pain receptors in the rabbit ear for the attack of bradykinin. Therefore, inhibition of PG-synthesis (i.e. by indomethacin) or inhibition of the sensitizing action of E-type PGs (i.e. by polyphloretin phosphate) reduces the pain producing effect of bradykinin. Since ACh releases only minimal amounts of E-type PGs, its effect is reduced only to a minimal extent by indomethacin or polyphloretin phosphate.", "PMID": 970294}
{"id": "pubmed23n0032_7890", "title": "Influence of adjuvant arthritis on connective-tissue metabolism.", "content": "In rats with adjuvant arthritis, an analysis was made of the metabolism of sulfated proteoglycans, with particular attention to the dorsal skin and the extremities. Alterations were demonstrated in all the tissues examined. In the uninflamed dorsal skin, the uptake of exogenous sulfate was diminished. By contrast, in both hind limbs, at the injected and the non-injected site, the turnover of sulfated mucopolysaccharides was increased, above all in ligament and cartilage; the calcium content of the bone tissue of both hind legs was, however, not affected by the disease.", "contents": "Influence of adjuvant arthritis on connective-tissue metabolism. In rats with adjuvant arthritis, an analysis was made of the metabolism of sulfated proteoglycans, with particular attention to the dorsal skin and the extremities. Alterations were demonstrated in all the tissues examined. In the uninflamed dorsal skin, the uptake of exogenous sulfate was diminished. By contrast, in both hind limbs, at the injected and the non-injected site, the turnover of sulfated mucopolysaccharides was increased, above all in ligament and cartilage; the calcium content of the bone tissue of both hind legs was, however, not affected by the disease.", "PMID": 970296}
{"id": "pubmed23n0032_7891", "title": "Polyphloretin phosphate reduces the algesic action of bradykinin by interfering with E-type prostaglandins.", "content": "(1) The method of the isolated perfused rabbit ear connected to the body by its nerve only was used to investigate the influence of the prostaglandin-antagonist polyphloretin phosphate (PPP) on the algesic effect of bradykinin (B) and acetylcholine (ACh). (2) Intra-arterial injections of B and ACh into the ear elicit a reflex fall in systemic blood pressure of the anaesthetized animal. PPP reduces this effects of B in proportion to the dose. The effect of ACh is reduced only to a small extent and only under higher concentrations of PPP than those necessary for inhibiting the effect of B. (3) Prostaglandin E1 (PGE1), when infused i.a. into the ear, enhances the effect of B and ACh by a sensitizing action on the perivascular pain receptors. PPP reduces or totally abolishes the PGE1-induced enhancement of the effect of B and ACh. (4) It is concluded that PPP reduces the effect of B mainly by inhibiting directly the pain enhancing action of the endogenously released PGs of the E-type. The effect of ACh is reduced only in the high concentration of PPP to a small extent probably by inhibiting the ACh-action rather than the sensitizing action of the only minimal released amounts of PGs. The PG-antagonizing action of PPP is further proven by the fact that during an additional infusion of PGE1 the enhanced effects of both B as well as ACh are reduced or abolished by PPP.", "contents": "Polyphloretin phosphate reduces the algesic action of bradykinin by interfering with E-type prostaglandins. (1) The method of the isolated perfused rabbit ear connected to the body by its nerve only was used to investigate the influence of the prostaglandin-antagonist polyphloretin phosphate (PPP) on the algesic effect of bradykinin (B) and acetylcholine (ACh). (2) Intra-arterial injections of B and ACh into the ear elicit a reflex fall in systemic blood pressure of the anaesthetized animal. PPP reduces this effects of B in proportion to the dose. The effect of ACh is reduced only to a small extent and only under higher concentrations of PPP than those necessary for inhibiting the effect of B. (3) Prostaglandin E1 (PGE1), when infused i.a. into the ear, enhances the effect of B and ACh by a sensitizing action on the perivascular pain receptors. PPP reduces or totally abolishes the PGE1-induced enhancement of the effect of B and ACh. (4) It is concluded that PPP reduces the effect of B mainly by inhibiting directly the pain enhancing action of the endogenously released PGs of the E-type. The effect of ACh is reduced only in the high concentration of PPP to a small extent probably by inhibiting the ACh-action rather than the sensitizing action of the only minimal released amounts of PGs. The PG-antagonizing action of PPP is further proven by the fact that during an additional infusion of PGE1 the enhanced effects of both B as well as ACh are reduced or abolished by PPP.", "PMID": 970295}
{"id": "pubmed23n0032_7892", "title": "Anti-inflammatory and related properties of 2-(2,4-dichlorophenoxy)phenylacetic acid (fenclofenac).", "content": "Fenclofenac was shown to possess anti-inflammatory, antinociceptive and antipyretic properties as measured by tests in rats that detect clinically active compounds. In a chronic test for assessing anti-inflammatory activity (established adjuvant arthritis), it was approximately equipotent to alclofenac, fenoprofen calcium and phenylbutazone, more potent than acetylsalicylic acid and ibuprofen, but was less potent than diclofenac sodium, indomethacin, ketoprofen and naproxen. In contrast, the potency of fenclofenac in acute tests for anti-inflammatory, antinociceptive and anti-pyretic activity was generally lower, the drug being approximately equipotent to acetylsalicylic acid in such tests. The anti-inflammatory activity of fenclofenac was not mediated via the pituitary-adrenal axis or a counter-irritant action. Fenclofenac was shown to have remarkably low gastric ulcerogenic potential, both acutely and chronically.", "contents": "Anti-inflammatory and related properties of 2-(2,4-dichlorophenoxy)phenylacetic acid (fenclofenac). Fenclofenac was shown to possess anti-inflammatory, antinociceptive and antipyretic properties as measured by tests in rats that detect clinically active compounds. In a chronic test for assessing anti-inflammatory activity (established adjuvant arthritis), it was approximately equipotent to alclofenac, fenoprofen calcium and phenylbutazone, more potent than acetylsalicylic acid and ibuprofen, but was less potent than diclofenac sodium, indomethacin, ketoprofen and naproxen. In contrast, the potency of fenclofenac in acute tests for anti-inflammatory, antinociceptive and anti-pyretic activity was generally lower, the drug being approximately equipotent to acetylsalicylic acid in such tests. The anti-inflammatory activity of fenclofenac was not mediated via the pituitary-adrenal axis or a counter-irritant action. Fenclofenac was shown to have remarkably low gastric ulcerogenic potential, both acutely and chronically.", "PMID": 970297}
{"id": "pubmed23n0032_7893", "title": "Computer-based approach to chelation therapy: a theoretical study of some chelating agents for the selective removal of toxic metal ions from plasma.", "content": "COMICS is a computer programme for calculating equilibrium concentrations of metal complexes and reactive species in multi-metal-multi-ligand systems. Its usefulness for analysing metal ion equilibria in blood plasma has been improved by including albumin as a ligand. Using this model system the distribution and removal of copper(II) and zinc ions in histidinaemia, lead poisoning and Wilson's disease have been examined. The efficacy of TRIEN in removing excess copper(II) is shown. The use of specific tripeptides such as Gly-Gly-His methyl ester for the selective removal of copper(II) is suggested. A possible chemoprophylaxis of influenza based on complexation of zinc is discussed. Calculations confirm that thiosemicarbazones such as methisazone and 2-acetylpyridine thiosemicarbazone are effective competitors for heavy metal ions under physiological conditions.", "contents": "Computer-based approach to chelation therapy: a theoretical study of some chelating agents for the selective removal of toxic metal ions from plasma. COMICS is a computer programme for calculating equilibrium concentrations of metal complexes and reactive species in multi-metal-multi-ligand systems. Its usefulness for analysing metal ion equilibria in blood plasma has been improved by including albumin as a ligand. Using this model system the distribution and removal of copper(II) and zinc ions in histidinaemia, lead poisoning and Wilson's disease have been examined. The efficacy of TRIEN in removing excess copper(II) is shown. The use of specific tripeptides such as Gly-Gly-His methyl ester for the selective removal of copper(II) is suggested. A possible chemoprophylaxis of influenza based on complexation of zinc is discussed. Calculations confirm that thiosemicarbazones such as methisazone and 2-acetylpyridine thiosemicarbazone are effective competitors for heavy metal ions under physiological conditions.", "PMID": 970298}
{"id": "pubmed23n0032_7894", "title": "A radioactive mercury vapor generating and exposing system for small scale animal experiments.", "content": "An apparatus is described for exposing small animals to high specific activity elemental mercury vapor. The vapor is generated by reduction of mercury chloride labelled with the 203HG isotope. The vapor is carried by a constant stream of air into the exposure chamber. The method has been used to expose rats or mice to constant air concentrations of mercury vapor in the range from 0.008 to 0.32 mg/m3. The specific activity of the mercuric chloride used in the study was approximately 1.8 mCi/mgHg. This is sufficiently high to allow measurement of tissue deposition of mercury after an exposure period of only 30 minutes.", "contents": "A radioactive mercury vapor generating and exposing system for small scale animal experiments. An apparatus is described for exposing small animals to high specific activity elemental mercury vapor. The vapor is generated by reduction of mercury chloride labelled with the 203HG isotope. The vapor is carried by a constant stream of air into the exposure chamber. The method has been used to expose rats or mice to constant air concentrations of mercury vapor in the range from 0.008 to 0.32 mg/m3. The specific activity of the mercuric chloride used in the study was approximately 1.8 mCi/mgHg. This is sufficiently high to allow measurement of tissue deposition of mercury after an exposure period of only 30 minutes.", "PMID": 970319}
{"id": "pubmed23n0032_7895", "title": "Measurement of protection factors of chemical cartridge, half-mask respirators under working conditions in a copper smelter.", "content": "The performance of three commonly used chemical cartridge respirators for SO2 was measured under working conditions in a copper smelter. An effective SO2 protection factor for each man-respirator trial was calculated as the ratio between average SO2 concentrations sampled outside the respirator mask and that simultaneously sampled from inside the mask. The in-use SO2 protection afforded by the three respirators proved to be highly variable with mean protection factors of 22, 18, and 13. The distributions of the protection factors and the effects of mask type, facial size, and mask comfort are discussed.", "contents": "Measurement of protection factors of chemical cartridge, half-mask respirators under working conditions in a copper smelter. The performance of three commonly used chemical cartridge respirators for SO2 was measured under working conditions in a copper smelter. An effective SO2 protection factor for each man-respirator trial was calculated as the ratio between average SO2 concentrations sampled outside the respirator mask and that simultaneously sampled from inside the mask. The in-use SO2 protection afforded by the three respirators proved to be highly variable with mean protection factors of 22, 18, and 13. The distributions of the protection factors and the effects of mask type, facial size, and mask comfort are discussed.", "PMID": 970320}
{"id": "pubmed23n0032_7896", "title": "Quantitating the efficiency of the coal mine personal respirable dust sampler for sampling oxide fumes.", "content": "Coal mine environments containing fume contaminants are assessed using the standard personal respirable dust sampler. The effect of the first-stage collector and filter on the total concentration measured was evaluated. The results showed that the personal respirable dust sampler could be used to sample fumes with TLV's greater than 1.0 mg/m3.", "contents": "Quantitating the efficiency of the coal mine personal respirable dust sampler for sampling oxide fumes. Coal mine environments containing fume contaminants are assessed using the standard personal respirable dust sampler. The effect of the first-stage collector and filter on the total concentration measured was evaluated. The results showed that the personal respirable dust sampler could be used to sample fumes with TLV's greater than 1.0 mg/m3.", "PMID": 970321}
{"id": "pubmed23n0032_7897", "title": "Air-purifying powered respirator pack utilizing a miniature two-stage air mover.", "content": "Design and construction of a moderate-weight, battery-powered, positive-pressure respirator pack is described. Design detail of a two-stage air mover is given, along with its adaptation for use as a portable air sampler.", "contents": "Air-purifying powered respirator pack utilizing a miniature two-stage air mover. Design and construction of a moderate-weight, battery-powered, positive-pressure respirator pack is described. Design detail of a two-stage air mover is given, along with its adaptation for use as a portable air sampler.", "PMID": 970322}
{"id": "pubmed23n0032_7898", "title": "Decision and estimation procedures for air contaminants.", "content": "This paper deals with the problem of enforcing average exposure standards for atmospheric contaminants in an industrial environment based upon short-period samples. Due to inherent uncertainties, a decision is to be made only if the probability of incorrect decision is below a prescribed level. It is shown how such a decision can be obtained in a very simple manner with the help of a decision chart with families of curves corresponding to the number of observations.", "contents": "Decision and estimation procedures for air contaminants. This paper deals with the problem of enforcing average exposure standards for atmospheric contaminants in an industrial environment based upon short-period samples. Due to inherent uncertainties, a decision is to be made only if the probability of incorrect decision is below a prescribed level. It is shown how such a decision can be obtained in a very simple manner with the help of a decision chart with families of curves corresponding to the number of observations.", "PMID": 970323}
{"id": "pubmed23n0032_7899", "title": "A statistical technique for determining compliance with dual hygienic standards.", "content": "A mathematical technique was developed to calculate log-normal distribution for sample results. This is then compared with a Limiting distribution defined by the sampling time, the Time-Weighted Average and the Ceiling Limit standard for the material tested. The technique provides a more effective means to decide if either or both the T.W.A. or Ceiling Limit has been exceeded. Sample times from 10 minutes to 4 hours are considered. A similar technique has previously been reported for evaluating benzene exposures. Examples are provided to illustrate the procedure.", "contents": "A statistical technique for determining compliance with dual hygienic standards. A mathematical technique was developed to calculate log-normal distribution for sample results. This is then compared with a Limiting distribution defined by the sampling time, the Time-Weighted Average and the Ceiling Limit standard for the material tested. The technique provides a more effective means to decide if either or both the T.W.A. or Ceiling Limit has been exceeded. Sample times from 10 minutes to 4 hours are considered. A similar technique has previously been reported for evaluating benzene exposures. Examples are provided to illustrate the procedure.", "PMID": 970324}
{"id": "pubmed23n0032_7900", "title": "The energetics of mask wear.", "content": "Experimental data further defining the region of predominant respiratory stress is presented. Within this region, energy requirements of respiration increase disproportionately to the overall energy expenditure of the body as excercise intensity increases. Addition of a mask accentuates this tendency, especially since no physiological compensation is possible in mask parameters. Mask technology has improved masks to the point where they no longer dominate the man-mask system, but instead contribute resistances and dead volumes approximately equal to those naturally occurring in man. Modeling base on minimization of respiratory energy rate of expenditure in the human is applied to this man-mask system and predictions are made which to this point have been nearly impossible to obtain from experimental data.", "contents": "The energetics of mask wear. Experimental data further defining the region of predominant respiratory stress is presented. Within this region, energy requirements of respiration increase disproportionately to the overall energy expenditure of the body as excercise intensity increases. Addition of a mask accentuates this tendency, especially since no physiological compensation is possible in mask parameters. Mask technology has improved masks to the point where they no longer dominate the man-mask system, but instead contribute resistances and dead volumes approximately equal to those naturally occurring in man. Modeling base on minimization of respiratory energy rate of expenditure in the human is applied to this man-mask system and predictions are made which to this point have been nearly impossible to obtain from experimental data.", "PMID": 970325}
{"id": "pubmed23n0032_7901", "title": "Serial measurements of left ventricular ejection fraction by radionuclide angiography early and late after myocardial infarction.", "content": "The left ventricular ejection fraction was determined serially with radioisotope angiography in 63 patients with acute myocardial infarction. After the peripheral injection of a bolus of technetium-99m, precordial radioactivity was recorded with a gamma scintillation camera and the ejection fraction calculated from the high frequency left ventricular time-activity curve. Since this technique requires no assumptions with respect to left ventricular geometry, it is particularly useful in patients with segmental left ventricular dysfunction. Serial measurements during the first 5 days after hospital admission were made in 50 patients, 30 of whom were studied during the subsequent 2 to 39 months (mean 19.9 months). Late follow-up serial studies were also performed in an additional 13 patients who had only one measurement of the left ventricular ejection fraction during the early postinfarction period. Early after infarction, the left ventricular ejection fraction was normal (more than 0.52) in only 15 of the 63 patients, and averaged 0.52 +/- 0.05 (standard deviation) in the 27 patients with an uncomplicated infarct. The ejection fraction was reduced in 24 patients with mild to moderate left ventricular failure (0.40 +/- 0.05, P less than 0.0001) and in the 12 patients with overt pulmonary edema (0.33 +/- 0.07, P less than 0.0001). In 35 patients the ejection fraction correlated with the mean pulmonary arterial wedge pressure (r = 0.72). In 15 patients with normal left ventricular wall motion by heart motion videotracking, the ejection fraction was significantly higher (0.53 +/- 0.08) than in the 26 patients with regional left ventricular dysfunction (0.41 +/- 0.10, P less than 0.0001). During the early postinfarction period, the left ventricular ejection fraction improved in 55 percent of patients and remained unchanged or decreased in 45 percent. A further increase in the ejection fraction was noted in 61 percent of patients during the late follow-up period. Patients with an initially low or decreasing ejection fraction had a significantly greater incidence of early mortality and left ventricular dysfunction (P less than 0.02) than those whose ejection fraction was normal or improved to normal early after infarction. These data indicate that the ejection fraction is a sensitive indicator of left ventricular function after acute myocardial infarction and that serial measurements are helpful in predicting early mortality and morbidity.", "contents": "Serial measurements of left ventricular ejection fraction by radionuclide angiography early and late after myocardial infarction. The left ventricular ejection fraction was determined serially with radioisotope angiography in 63 patients with acute myocardial infarction. After the peripheral injection of a bolus of technetium-99m, precordial radioactivity was recorded with a gamma scintillation camera and the ejection fraction calculated from the high frequency left ventricular time-activity curve. Since this technique requires no assumptions with respect to left ventricular geometry, it is particularly useful in patients with segmental left ventricular dysfunction. Serial measurements during the first 5 days after hospital admission were made in 50 patients, 30 of whom were studied during the subsequent 2 to 39 months (mean 19.9 months). Late follow-up serial studies were also performed in an additional 13 patients who had only one measurement of the left ventricular ejection fraction during the early postinfarction period. Early after infarction, the left ventricular ejection fraction was normal (more than 0.52) in only 15 of the 63 patients, and averaged 0.52 +/- 0.05 (standard deviation) in the 27 patients with an uncomplicated infarct. The ejection fraction was reduced in 24 patients with mild to moderate left ventricular failure (0.40 +/- 0.05, P less than 0.0001) and in the 12 patients with overt pulmonary edema (0.33 +/- 0.07, P less than 0.0001). In 35 patients the ejection fraction correlated with the mean pulmonary arterial wedge pressure (r = 0.72). In 15 patients with normal left ventricular wall motion by heart motion videotracking, the ejection fraction was significantly higher (0.53 +/- 0.08) than in the 26 patients with regional left ventricular dysfunction (0.41 +/- 0.10, P less than 0.0001). During the early postinfarction period, the left ventricular ejection fraction improved in 55 percent of patients and remained unchanged or decreased in 45 percent. A further increase in the ejection fraction was noted in 61 percent of patients during the late follow-up period. Patients with an initially low or decreasing ejection fraction had a significantly greater incidence of early mortality and left ventricular dysfunction (P less than 0.02) than those whose ejection fraction was normal or improved to normal early after infarction. These data indicate that the ejection fraction is a sensitive indicator of left ventricular function after acute myocardial infarction and that serial measurements are helpful in predicting early mortality and morbidity.", "PMID": 970327}
{"id": "pubmed23n0032_7902", "title": "Regional myocardial blood flow during hyperemia induced by contrast agent in patients with coronary artery disease.", "content": "Regional myocardial specific blood flow (regional specific flow) was measured at rest and during contrast hyperemia after the intracoronary injection of xenon-133. The changes in regional specific flow were transient, resulting in some compromise in one of the underlying restraints of the inert gas washout method, namely, the presence of a steady state. Therefore, to determine the clinical utility of this technique, regional specific flow values obtained with this method were correlated with the presence and severity of coronary artery disease as assessed from the coronary arteriogram and left ventriculogram. Regional specific flow during contrast hyperemia was 186+/- 11 (mean +/- 1 standard error of the mean) ml/min per 100 g in control patients and 115+/-5 in patients with coronary artery disease. There was an inverse relation between regional specific flow during contrast hyperemia and the percent coronary stenosis when the stenosis was 40 percent or greater (r = 0.70, P less than 0.001). Regional specific flow was significantly less in patients with asynergy (77 +/- 10 ml/min per 100 g) than in patients with normal ventricular function (105 +/- 5) distal to coronary stenoses of greater than 75 percent. Thus regional specific flow measured during contrast hyperemia using the xenon washout technique and the Anger camera differentiated patients with normal coronary arteriograms from those with coronary artery disease. With this technique, good correlation was shown between regional specific flow and the percent coronary stenosis and presence of ventricular wall abnormalities. The information obtained with this method may provide prognostic information concerning suitability for surgical intervention.", "contents": "Regional myocardial blood flow during hyperemia induced by contrast agent in patients with coronary artery disease. Regional myocardial specific blood flow (regional specific flow) was measured at rest and during contrast hyperemia after the intracoronary injection of xenon-133. The changes in regional specific flow were transient, resulting in some compromise in one of the underlying restraints of the inert gas washout method, namely, the presence of a steady state. Therefore, to determine the clinical utility of this technique, regional specific flow values obtained with this method were correlated with the presence and severity of coronary artery disease as assessed from the coronary arteriogram and left ventriculogram. Regional specific flow during contrast hyperemia was 186+/- 11 (mean +/- 1 standard error of the mean) ml/min per 100 g in control patients and 115+/-5 in patients with coronary artery disease. There was an inverse relation between regional specific flow during contrast hyperemia and the percent coronary stenosis when the stenosis was 40 percent or greater (r = 0.70, P less than 0.001). Regional specific flow was significantly less in patients with asynergy (77 +/- 10 ml/min per 100 g) than in patients with normal ventricular function (105 +/- 5) distal to coronary stenoses of greater than 75 percent. Thus regional specific flow measured during contrast hyperemia using the xenon washout technique and the Anger camera differentiated patients with normal coronary arteriograms from those with coronary artery disease. With this technique, good correlation was shown between regional specific flow and the percent coronary stenosis and presence of ventricular wall abnormalities. The information obtained with this method may provide prognostic information concerning suitability for surgical intervention.", "PMID": 970328}
{"id": "pubmed23n0032_7903", "title": "Noninvasive myocardial imaging with potassium-43 and rubidium-81 in patients with left bundle branch block.", "content": "Noninvasive myocardial imaging with potassium-43 and rubidium-81 has been used successfully to identify areas of infarction and exercise-induced ischemia as regions of decreased radioactivity. The image defects observed are believed to be due to a decreased radionuclide uptake in regions of myocardial scar or to heterogeneous myocardial accumulation of tracer as a result of regional ischemia. Of 27 patients with left bundle branch block studied with noninvasive imaging at rest and during exercise, 25 manifested at rest reduced radioactivity in the region of the interventricular septum. This pattern is similar to that seen in patients with anteroseptal myocardial infarction. Sixteen of the 27 patients underwent diagnostic coronary arteriography and left ventriculography. Only five of these patients had evidence of either previous infarction or significant obstructive coronary artery disease as assessed with clinical or angiographic criteria, or both. Although the image defect was routinely demonstrated at rest in patients with left bundle branch block, this defect was generally normalized or less distinct with exercise in patients with no anatomic heart disease. In contrast, a larger, more distinct or new image defect with exercise correctly identified the presence of significant obstructive coronary artery disease in patients with left bundle branch block. In the clinical application of noninvasive myocardial imaging, these image defects observed at rest can lead to the false pasitive radionuclide interpretation of anteroseptal myocardial infarction.", "contents": "Noninvasive myocardial imaging with potassium-43 and rubidium-81 in patients with left bundle branch block. Noninvasive myocardial imaging with potassium-43 and rubidium-81 has been used successfully to identify areas of infarction and exercise-induced ischemia as regions of decreased radioactivity. The image defects observed are believed to be due to a decreased radionuclide uptake in regions of myocardial scar or to heterogeneous myocardial accumulation of tracer as a result of regional ischemia. Of 27 patients with left bundle branch block studied with noninvasive imaging at rest and during exercise, 25 manifested at rest reduced radioactivity in the region of the interventricular septum. This pattern is similar to that seen in patients with anteroseptal myocardial infarction. Sixteen of the 27 patients underwent diagnostic coronary arteriography and left ventriculography. Only five of these patients had evidence of either previous infarction or significant obstructive coronary artery disease as assessed with clinical or angiographic criteria, or both. Although the image defect was routinely demonstrated at rest in patients with left bundle branch block, this defect was generally normalized or less distinct with exercise in patients with no anatomic heart disease. In contrast, a larger, more distinct or new image defect with exercise correctly identified the presence of significant obstructive coronary artery disease in patients with left bundle branch block. In the clinical application of noninvasive myocardial imaging, these image defects observed at rest can lead to the false pasitive radionuclide interpretation of anteroseptal myocardial infarction.", "PMID": 970329}
{"id": "pubmed23n0032_7904", "title": "Reduction of S-T segment elevation with infusion of nitroprusside in patients with acute myocardial infarction.", "content": "The effect of infusion of sodium nitroprusside on S-T segment elevation was evaluated in 12 patients with acute anterior myocardial infarction. Precordial 35 lead S-T segment maps were obtained in each patient immediately before and 10 minutes after infusion of 53 mug/min (range 20 to 100 mug/min) of nitroprusside. The following measurements were made from each S-T map: sigmaST (total S-T elevation in all leads), NST (number of leads with S-T elevation greater than 1 mm) and ST (average (S-T elevation in leads with more than 1 mm elevation). After administration of mitroprusside, evidence of myocardial ischemic injury as assessed by S-T mapping decreased in association with reduction of the myocardial oxygen consumption index of pressure-time per minute. Group mean values diminished significantly for sigmaST (41.7 to 28.6 mm, P less than 0.001), NST (20.3 to 14.6, P less than 0.001) and ST (1.6 to 1.2 mm, P less than 0.005). Pressure-time per minute decreased from 2,690 to 2,372 mm Hg-sec/min (P less than 0.001). Because there was no significant relation (P less than 0.05) between reductions in S-T elevation and lower indexes of myocardial oxygen consumption, it is suggested that nitroprusside may possess a separate action of augmenting regional blood flow to ischemic myocardium. Evaluation with the precordial S-T mapping technique suggested that intravenous administration of nitroprusside was associated with evidence of reduced ventricular ischemic injury in patients with acute myocardial infarction. This effect appears to be related to reduction of myocardial oxygen demand by the peripheral cardiac unloading mechanisms of nitroprusside as well as to a possible direct action of the drug in improving regional blood flow to ischemic heart muscle.", "contents": "Reduction of S-T segment elevation with infusion of nitroprusside in patients with acute myocardial infarction. The effect of infusion of sodium nitroprusside on S-T segment elevation was evaluated in 12 patients with acute anterior myocardial infarction. Precordial 35 lead S-T segment maps were obtained in each patient immediately before and 10 minutes after infusion of 53 mug/min (range 20 to 100 mug/min) of nitroprusside. The following measurements were made from each S-T map: sigmaST (total S-T elevation in all leads), NST (number of leads with S-T elevation greater than 1 mm) and ST (average (S-T elevation in leads with more than 1 mm elevation). After administration of mitroprusside, evidence of myocardial ischemic injury as assessed by S-T mapping decreased in association with reduction of the myocardial oxygen consumption index of pressure-time per minute. Group mean values diminished significantly for sigmaST (41.7 to 28.6 mm, P less than 0.001), NST (20.3 to 14.6, P less than 0.001) and ST (1.6 to 1.2 mm, P less than 0.005). Pressure-time per minute decreased from 2,690 to 2,372 mm Hg-sec/min (P less than 0.001). Because there was no significant relation (P less than 0.05) between reductions in S-T elevation and lower indexes of myocardial oxygen consumption, it is suggested that nitroprusside may possess a separate action of augmenting regional blood flow to ischemic myocardium. Evaluation with the precordial S-T mapping technique suggested that intravenous administration of nitroprusside was associated with evidence of reduced ventricular ischemic injury in patients with acute myocardial infarction. This effect appears to be related to reduction of myocardial oxygen demand by the peripheral cardiac unloading mechanisms of nitroprusside as well as to a possible direct action of the drug in improving regional blood flow to ischemic heart muscle.", "PMID": 970330}
{"id": "pubmed23n0032_7905", "title": "Variation in the size and distensibility of the pulmonary arteries in d-transposition of the great arteries.", "content": "The relative size of the main pulmonary artery was determined from cineangiograms of 117 patients with d-transposition of the great arteries by calculating the ration between the diameters of the main pulmonary artery and aorta. The pulmonary artery was largest in patients with ventricular septal defect or patent ductus arteriosus, or both, because of increased pulmonary arterial pressure and flow. In patients with an intact ventricular septum or with left ventricular outflow tract obstruction, or both, the main pulmonary artery was approximately the size of the aorta. Two cases of d-transposition and gross dilatation of the main pulmonary artery and hypoplastic first branch pulmonary arteries are presented. In these cases the ratio between the diameters of the main pulmonary artery and aorta was greater than in any of the other 117 cases studied...", "contents": "Variation in the size and distensibility of the pulmonary arteries in d-transposition of the great arteries. The relative size of the main pulmonary artery was determined from cineangiograms of 117 patients with d-transposition of the great arteries by calculating the ration between the diameters of the main pulmonary artery and aorta. The pulmonary artery was largest in patients with ventricular septal defect or patent ductus arteriosus, or both, because of increased pulmonary arterial pressure and flow. In patients with an intact ventricular septum or with left ventricular outflow tract obstruction, or both, the main pulmonary artery was approximately the size of the aorta. Two cases of d-transposition and gross dilatation of the main pulmonary artery and hypoplastic first branch pulmonary arteries are presented. In these cases the ratio between the diameters of the main pulmonary artery and aorta was greater than in any of the other 117 cases studied...", "PMID": 970331}
{"id": "pubmed23n0032_7906", "title": "Surgical treatment of degenerative mitral regurgitation.", "content": "Between October 1965 and April 1975, mitral valve replacement was preformed in 66 patients with myxomatous degeneration of the mitral valve (\"floppy valve syndrome\"). Operative mortality was 6 percent (four patients). Current evaluation was obtained for all patients; the average postoperative follow-up interval for surviving patients was 3.5 years (range 1 month to 9.9 years); the total duration of postoperative follow-up for all patients was 180 patient-years. Overall survival rates, calculated by the actuarial method, were 81, 68 and 50 percent, respectively, 1, 2 and 5 years after mitral valve replacement. Preoperative variables with a significantly adverse effect on patient survival included patient age greater than 50 years, New York Heart Association functional class IV, left ventricular end-diastolic pressure greater than 12 mm Hg and mean pulmonary arterial wedge pressure greater than 16 mm Hg. Support is advanced for the concept that mitral valve dysfunction associated with myxomatous degeneration constitutes a broad spectrum of clinicopathologic involvement. Acute clinical and hemodynamic deterioration may often occur in the setting of chronic mitral valve dysfunction. Postoperative mortality is directly related to preoperative functional disability and hemodynamic evidence of impaired left ventricular function. Consideration should be given to earlier operative intervention in patients with myoxmatous mitral degeneration and mitral insufficiency before severe and probably irreversible impairment of ventricular function occurs.", "contents": "Surgical treatment of degenerative mitral regurgitation. Between October 1965 and April 1975, mitral valve replacement was preformed in 66 patients with myxomatous degeneration of the mitral valve (\"floppy valve syndrome\"). Operative mortality was 6 percent (four patients). Current evaluation was obtained for all patients; the average postoperative follow-up interval for surviving patients was 3.5 years (range 1 month to 9.9 years); the total duration of postoperative follow-up for all patients was 180 patient-years. Overall survival rates, calculated by the actuarial method, were 81, 68 and 50 percent, respectively, 1, 2 and 5 years after mitral valve replacement. Preoperative variables with a significantly adverse effect on patient survival included patient age greater than 50 years, New York Heart Association functional class IV, left ventricular end-diastolic pressure greater than 12 mm Hg and mean pulmonary arterial wedge pressure greater than 16 mm Hg. Support is advanced for the concept that mitral valve dysfunction associated with myxomatous degeneration constitutes a broad spectrum of clinicopathologic involvement. Acute clinical and hemodynamic deterioration may often occur in the setting of chronic mitral valve dysfunction. Postoperative mortality is directly related to preoperative functional disability and hemodynamic evidence of impaired left ventricular function. Consideration should be given to earlier operative intervention in patients with myoxmatous mitral degeneration and mitral insufficiency before severe and probably irreversible impairment of ventricular function occurs.", "PMID": 970332}
{"id": "pubmed23n0032_7907", "title": "Echocardiographic evaluation of tricuspid valve endocarditis: an M mode and two dimensional study.", "content": "Standard M mode echocardiography and a new real time two dimensional echocardiographic imaging system were utilized to follow the progressive anatomic destruction of the tricuspid valve in a patient with bacterial endocarditis. The initial two dimensional echocardiographic study revealed large vegetative masses attached to severely prolapsing tricuspid leaflets. Serial studies demonstrated eventual disruption of the chordal attachments of the anterior tricuspid leaflet resulting in frank leaflet flail. This technique was seen to complement both M mode echocardiography and cardiac angiography by providing spatial information concerning serial changes in the disordered tricuspid valve. Such findings call attention to the relative roles of various diagnostic measures, including M mode and two dimensional echocardiography, in assessing the specific anatomic and functional performance of a diseased tricuspid valve.", "contents": "Echocardiographic evaluation of tricuspid valve endocarditis: an M mode and two dimensional study. Standard M mode echocardiography and a new real time two dimensional echocardiographic imaging system were utilized to follow the progressive anatomic destruction of the tricuspid valve in a patient with bacterial endocarditis. The initial two dimensional echocardiographic study revealed large vegetative masses attached to severely prolapsing tricuspid leaflets. Serial studies demonstrated eventual disruption of the chordal attachments of the anterior tricuspid leaflet resulting in frank leaflet flail. This technique was seen to complement both M mode echocardiography and cardiac angiography by providing spatial information concerning serial changes in the disordered tricuspid valve. Such findings call attention to the relative roles of various diagnostic measures, including M mode and two dimensional echocardiography, in assessing the specific anatomic and functional performance of a diseased tricuspid valve.", "PMID": 970333}
{"id": "pubmed23n0032_7908", "title": "Quinidine-induced ventricular flutter and fibrillation without digitalis therapy.", "content": "Three cases are described with documented ventricular flutter and fibrillation during quinidine medication without concomitant digitalis therapy. In all three patients the arrhythmia developed while they were receiving moderate doses of quinidine. Although no changes in QRS width were observed after administration of quinidine, there was marked prolongation of the Q-T interval. The mechanism of development of ventricular flutter and fibrillation in patients taking quinidine may be similar to that in patients with the Q-T prolongation syndrome.", "contents": "Quinidine-induced ventricular flutter and fibrillation without digitalis therapy. Three cases are described with documented ventricular flutter and fibrillation during quinidine medication without concomitant digitalis therapy. In all three patients the arrhythmia developed while they were receiving moderate doses of quinidine. Although no changes in QRS width were observed after administration of quinidine, there was marked prolongation of the Q-T interval. The mechanism of development of ventricular flutter and fibrillation in patients taking quinidine may be similar to that in patients with the Q-T prolongation syndrome.", "PMID": 970334}
{"id": "pubmed23n0032_7909", "title": "Functional anatomy of the hands of fur seals and sea lions.", "content": "Dissections and manipulations of the hands of 14 specimens of four genera of fur seals and sea lions and of generalized fissiped carnivores were used to identify the structural modifications involved in formation of the expanded forelimb paddles characteristic of these pinnipeds. The morphological peculiarities were then correlated with differences in locomotor function, both on land and in the water, on the basis of previously published data, for the purpose of identifying modifications of structure and function which have adaptive vale. Structural differences found in the manus of fur seals and sea lions include: (1) reduction in size of the ulnar side of the carpus and a radial shift in the length-order of the digits, (2) development of musculature in the antebrachial fascia which attaches to the caudal margin of the flipper, (3) orientation of the radial side of the manus dorsal and radial to the rest of the hand, (4) increased range of possible midcarpal movement and in deviational mobility at the first and fifth digits, (5) attachment of forearm musculature onto radial digits and (6) well-developed hypothenar muscles and absence of thenar muscles. Modifications in hand structure are viewed as providing a morphological basis for employment of the hand to advantage during aquatic locomotion while maintaining thrust-producing and potentially energy-conserving capacities during movement on land. As such these differences in structure and function are viewed as adaptations to locomotion in the water and on land which are extensions of locomotor adaptations attributed to modifications of forelimb structure and function associated with the generation of massive aquatic locomotor thrust. The adaptive value of such modifications is interpreted as allowing fur seals and sea lions to swim with speed and thereby capture elusive fast-swimming prey while maintaining a level of terrestrial locomotor ability compatible with their amphibious mode of life.", "contents": "Functional anatomy of the hands of fur seals and sea lions. Dissections and manipulations of the hands of 14 specimens of four genera of fur seals and sea lions and of generalized fissiped carnivores were used to identify the structural modifications involved in formation of the expanded forelimb paddles characteristic of these pinnipeds. The morphological peculiarities were then correlated with differences in locomotor function, both on land and in the water, on the basis of previously published data, for the purpose of identifying modifications of structure and function which have adaptive vale. Structural differences found in the manus of fur seals and sea lions include: (1) reduction in size of the ulnar side of the carpus and a radial shift in the length-order of the digits, (2) development of musculature in the antebrachial fascia which attaches to the caudal margin of the flipper, (3) orientation of the radial side of the manus dorsal and radial to the rest of the hand, (4) increased range of possible midcarpal movement and in deviational mobility at the first and fifth digits, (5) attachment of forearm musculature onto radial digits and (6) well-developed hypothenar muscles and absence of thenar muscles. Modifications in hand structure are viewed as providing a morphological basis for employment of the hand to advantage during aquatic locomotion while maintaining thrust-producing and potentially energy-conserving capacities during movement on land. As such these differences in structure and function are viewed as adaptations to locomotion in the water and on land which are extensions of locomotor adaptations attributed to modifications of forelimb structure and function associated with the generation of massive aquatic locomotor thrust. The adaptive value of such modifications is interpreted as allowing fur seals and sea lions to swim with speed and thereby capture elusive fast-swimming prey while maintaining a level of terrestrial locomotor ability compatible with their amphibious mode of life.", "PMID": 970340}
{"id": "pubmed23n0032_7910", "title": "Surface view of an injected human lung shortly before the fetus becomes viable.", "content": "These figures of a silicon-rubber-injected fetal human lung illustrate the precocious development of filaments (future alveolar ducts) of the pulmonary acini. In contrast, there is a long delay in the differentiation of respiratory bronchioles. The former are the first to sustain respiration.", "contents": "Surface view of an injected human lung shortly before the fetus becomes viable. These figures of a silicon-rubber-injected fetal human lung illustrate the precocious development of filaments (future alveolar ducts) of the pulmonary acini. In contrast, there is a long delay in the differentiation of respiratory bronchioles. The former are the first to sustain respiration.", "PMID": 970341}
{"id": "pubmed23n0032_7911", "title": "Anastomosing tubular arrangement of the exocrine pancreas.", "content": "Wax reconstructions of zymogen-containing cells in rat show the arrangement of the exocrine pancreas to be a branching, anastomosing system of tubules which vary in diameter and end blindly. This arrangement is not that of a true acinar gland.", "contents": "Anastomosing tubular arrangement of the exocrine pancreas. Wax reconstructions of zymogen-containing cells in rat show the arrangement of the exocrine pancreas to be a branching, anastomosing system of tubules which vary in diameter and end blindly. This arrangement is not that of a true acinar gland.", "PMID": 970342}
{"id": "pubmed23n0032_7912", "title": "Formation of packaging units in circket late spermatid nuclei as visualized by spreading techniques (1).", "content": "The nuclear organization of a particular step of cricket late spermiogenesis was examined by an electron microscope study of spermatids dispersed by air-liquid surface tension. Cell spreading techniques facilitate a display of condensing spermatid nuclei sufficient to allow interpretation of chromatin packaging processes. Results indicate that nuclei of late developing cricket spermatids are integrated by multiple packaging units as the result of an orderly aggregation of individual chromatin fibers. Each packaging unit consists of a thick fasicle, formed by the alignment of smooth chromatin fibers, which frays out into tassels of looped fibers.", "contents": "Formation of packaging units in circket late spermatid nuclei as visualized by spreading techniques (1). The nuclear organization of a particular step of cricket late spermiogenesis was examined by an electron microscope study of spermatids dispersed by air-liquid surface tension. Cell spreading techniques facilitate a display of condensing spermatid nuclei sufficient to allow interpretation of chromatin packaging processes. Results indicate that nuclei of late developing cricket spermatids are integrated by multiple packaging units as the result of an orderly aggregation of individual chromatin fibers. Each packaging unit consists of a thick fasicle, formed by the alignment of smooth chromatin fibers, which frays out into tassels of looped fibers.", "PMID": 970343}
{"id": "pubmed23n0032_7913", "title": "Glucagon levels in pancreatic extracts and plasma of the lizard (1).", "content": "Levels of glucagon in the splenic pancreas and in plasma of the lizard Anolis carolinensis were estimated by radioimmunoassay. The splenic pancreas of Anolis has a glucagon concentration nearly a 1000 times greater, on a weight basis, than that of the mammalian pancreas. Glucagon-like immunoreactivity (GLI) of anolian plasma varied over a wide range, but relative to mammals the GLI levels were inappropriately elevated for the concentration of plasma glucagon secretion, particularly as related to alpha cell function in the diabetic state.", "contents": "Glucagon levels in pancreatic extracts and plasma of the lizard (1). Levels of glucagon in the splenic pancreas and in plasma of the lizard Anolis carolinensis were estimated by radioimmunoassay. The splenic pancreas of Anolis has a glucagon concentration nearly a 1000 times greater, on a weight basis, than that of the mammalian pancreas. Glucagon-like immunoreactivity (GLI) of anolian plasma varied over a wide range, but relative to mammals the GLI levels were inappropriately elevated for the concentration of plasma glucagon secretion, particularly as related to alpha cell function in the diabetic state.", "PMID": 970344}
{"id": "pubmed23n0032_7914", "title": "Hydronephrosis in the mouse: the effects of the short-ear gene, sex and ureteral vascular system.", "content": "The short-ear gene in the mouse, se, affects a number of soft tissues; skeletal effects result in reduced body cavities. A high incidence of hydronephrosis in short-ear genotypes has been ascribed to pressure on the lower ureter resulting from crowding of organs in the body cavity. This study concerns the ureteral vascular system as observed in 861 autopsies of mice of varying age, of the three short-ear genotypes, drawn from ten stocks. Incidence and expression of hydronephrosis is greater in short-ear males than in females, and the ratio between the two varies from one stock to another. This study seeks an explanation. Short-ear genotypes have an abnormally high incidence of unusual ureteral veins, hydronephrosis being strongly associated with the more posterior ones. Ureteraonadal and iliac veins. The spermatic vein's more posterior position, compared with the ovarian, is strongly correlated with the sex difference in posterior ureteral venation and thus with the sex difference in incidence of hydronephrosis. Stocks are compared according to the amount of selection for fitness of short-ear genotypes; there is a reduced incidence of hydronephrosis with selection, accompanied by fewer posterior ureteral veins. Age also affects hydronephrosis; its expression increases slightly with age, and the male sese progeny of young mothers appear to be particularly prone to hydronephrosis. Three skeletal mutants segregate; of these fidget, fi, causes a type of hydronephrosis differing somewhat from that caused by se.", "contents": "Hydronephrosis in the mouse: the effects of the short-ear gene, sex and ureteral vascular system. The short-ear gene in the mouse, se, affects a number of soft tissues; skeletal effects result in reduced body cavities. A high incidence of hydronephrosis in short-ear genotypes has been ascribed to pressure on the lower ureter resulting from crowding of organs in the body cavity. This study concerns the ureteral vascular system as observed in 861 autopsies of mice of varying age, of the three short-ear genotypes, drawn from ten stocks. Incidence and expression of hydronephrosis is greater in short-ear males than in females, and the ratio between the two varies from one stock to another. This study seeks an explanation. Short-ear genotypes have an abnormally high incidence of unusual ureteral veins, hydronephrosis being strongly associated with the more posterior ones. Ureteraonadal and iliac veins. The spermatic vein's more posterior position, compared with the ovarian, is strongly correlated with the sex difference in posterior ureteral venation and thus with the sex difference in incidence of hydronephrosis. Stocks are compared according to the amount of selection for fitness of short-ear genotypes; there is a reduced incidence of hydronephrosis with selection, accompanied by fewer posterior ureteral veins. Age also affects hydronephrosis; its expression increases slightly with age, and the male sese progeny of young mothers appear to be particularly prone to hydronephrosis. Three skeletal mutants segregate; of these fidget, fi, causes a type of hydronephrosis differing somewhat from that caused by se.", "PMID": 970345}
{"id": "pubmed23n0032_7915", "title": "Fine structure of satellite cells in growing skeletal muscle.", "content": "The morphology of satellite cells was investigated in skeletal muscle from mice of various ages between 7 days and 50 weeks. Satellite cells of very young muscle had abundant cytoplasm which was rich in organelles. Free ribosomes were abundant and usually arranged into polysomes of 5-6 units. Cisternae of rough endoplasmic reticulum were heavily studded with ribosomes and occupied the polar regions of the cytoplasm. Marked dilations of the cisternae, filled with an amorphous electron-lucent material, were a frequent and characteristic feature of satellite cells of very young muscle. The cytoplasm of young cells also contained a well developed Golgi apparatus as well as numerous mitochondria, microfilaments and microtubules. With increasing age there was a rapid reduction in organelles both qualitatively and quantitatively. For rxample, as the number of ribosomes decreased, their organization into polysomes was lost. The rough endoplasmic reticulum was present in cells of older muscle merely as small isolated profiles that lacked dilations. These and other features demonstrated during this study are consistent with the concept that satellite cells are metabolically very active in young muscle but rapidly become quiescent as the animal grows older.", "contents": "Fine structure of satellite cells in growing skeletal muscle. The morphology of satellite cells was investigated in skeletal muscle from mice of various ages between 7 days and 50 weeks. Satellite cells of very young muscle had abundant cytoplasm which was rich in organelles. Free ribosomes were abundant and usually arranged into polysomes of 5-6 units. Cisternae of rough endoplasmic reticulum were heavily studded with ribosomes and occupied the polar regions of the cytoplasm. Marked dilations of the cisternae, filled with an amorphous electron-lucent material, were a frequent and characteristic feature of satellite cells of very young muscle. The cytoplasm of young cells also contained a well developed Golgi apparatus as well as numerous mitochondria, microfilaments and microtubules. With increasing age there was a rapid reduction in organelles both qualitatively and quantitatively. For rxample, as the number of ribosomes decreased, their organization into polysomes was lost. The rough endoplasmic reticulum was present in cells of older muscle merely as small isolated profiles that lacked dilations. These and other features demonstrated during this study are consistent with the concept that satellite cells are metabolically very active in young muscle but rapidly become quiescent as the animal grows older.", "PMID": 970346}
{"id": "pubmed23n0032_7916", "title": "Osteoligamentous guidance of the movements of the human thumb.", "content": "On examination of the thumbs of 20 dissected preparations of ligaments and joints, of ten dry skeletons and of a number of living hands, apoposition (from apo = away from) was distinguished as a position in which the first carpometacarpal joint is fully abducted and laterally rotated and in which one or both distal joints of the thumb are flexed. Apoposition is commonly used in writing and it has a specific osteoligamentous basis for its stability: (1) a Y-shaped intermetacarpal ligament is attached by two crura to the base of the second metacarpal bone and by a common stem to the first metacarpal. Together with the palmar and dorsal oblique ligaments it becomes taut at abduction and establishes thereby a fixed center for the circumduction. Stability is enhanced as the circumduction takes place in the radial flat part of the joint away from the center; (2) of the two palmar prominences of the head of the first metacarpal bone the radial is the larger. At 25-30 degrees flexion in the metacarpophalangeal joint the prominence fits into an excavation on the base of the proximal phalanx in a manner which together with the ulnar collateral ligament locks the joint against mutual abduction and lateral rotation, and (3) the radial part of the trochlea of the interphalangeal joint is larger than the ulnar and secures, together with the ulnar collateral ligament, the joint against a radial luxation. Apoposition does not require activity of the thumb muscles; it is brought about by applying an external force to the ulnar side of the thumb and it is checked by ligaments and the shape of the joints.", "contents": "Osteoligamentous guidance of the movements of the human thumb. On examination of the thumbs of 20 dissected preparations of ligaments and joints, of ten dry skeletons and of a number of living hands, apoposition (from apo = away from) was distinguished as a position in which the first carpometacarpal joint is fully abducted and laterally rotated and in which one or both distal joints of the thumb are flexed. Apoposition is commonly used in writing and it has a specific osteoligamentous basis for its stability: (1) a Y-shaped intermetacarpal ligament is attached by two crura to the base of the second metacarpal bone and by a common stem to the first metacarpal. Together with the palmar and dorsal oblique ligaments it becomes taut at abduction and establishes thereby a fixed center for the circumduction. Stability is enhanced as the circumduction takes place in the radial flat part of the joint away from the center; (2) of the two palmar prominences of the head of the first metacarpal bone the radial is the larger. At 25-30 degrees flexion in the metacarpophalangeal joint the prominence fits into an excavation on the base of the proximal phalanx in a manner which together with the ulnar collateral ligament locks the joint against mutual abduction and lateral rotation, and (3) the radial part of the trochlea of the interphalangeal joint is larger than the ulnar and secures, together with the ulnar collateral ligament, the joint against a radial luxation. Apoposition does not require activity of the thumb muscles; it is brought about by applying an external force to the ulnar side of the thumb and it is checked by ligaments and the shape of the joints.", "PMID": 970347}
{"id": "pubmed23n0032_7917", "title": "The autonomic innervation of the interstitial gland of the rat ovary during pregnancy.", "content": "The autonomic innervation of the interstitial gland of the rat ovary was studied on days 4, 6, 10, 14 and 18 of pregnancy with the acetylcholinesterase procedure, the Falck-Hillarp technique and electron microscopy after 5-hydroxydopamine treatment. Acetylcholinesterase-positive nerves were present as perivascular plexuses at all stages studied. Adrenergic nerves were present in the interstitial gland in all stages studied. The number and intensity of interstitial fluorescent adrenergic nerves increased as pregnancy progressed. Measurement of norepinephrine with the fluorometric procedure showed a highly significant (p less than 0.05) increase in the neurotransmitter in the ovary on days 14 and 18 as compared to day 4. Fine-structural studies after administration of the false transmitter, 5-hydroxydopamine, showed that the innervation of the steroidogenic cells of the interstitial gland is adrenergic.", "contents": "The autonomic innervation of the interstitial gland of the rat ovary during pregnancy. The autonomic innervation of the interstitial gland of the rat ovary was studied on days 4, 6, 10, 14 and 18 of pregnancy with the acetylcholinesterase procedure, the Falck-Hillarp technique and electron microscopy after 5-hydroxydopamine treatment. Acetylcholinesterase-positive nerves were present as perivascular plexuses at all stages studied. Adrenergic nerves were present in the interstitial gland in all stages studied. The number and intensity of interstitial fluorescent adrenergic nerves increased as pregnancy progressed. Measurement of norepinephrine with the fluorometric procedure showed a highly significant (p less than 0.05) increase in the neurotransmitter in the ovary on days 14 and 18 as compared to day 4. Fine-structural studies after administration of the false transmitter, 5-hydroxydopamine, showed that the innervation of the steroidogenic cells of the interstitial gland is adrenergic.", "PMID": 970348}
{"id": "pubmed23n0032_7918", "title": "The effects of 5-bromodeoxyuridine and isoproterenol on the postnatal differentiation of rat submandibular gland.", "content": "The effects of 5-bromodeoxyuridine (BrdU) on the postnatal differentiation of rat submandibular gland and on the isoproterenol-induced changes of differentiation were studied. The rats were injected with BrdU for three consecutive days, beginning at two days of age. The total dose of BrdU was 0.9 mg/g body weight. BrdU caused a severe retardation of growth up to 15 days of age. A rapid growth of the animals between 15 and 22 days indicated a recovery from the effect of BrdU. The growth of the submandibular gland was retarded similarly with a corresponding decrease in DNA, RNA and protein content. Incorporation of tritiated thymidine into the submandibular gland was not altered in the BrdU-treated animals at one and three days after the last injection of the analog. At days 15 and 22 the rate of thymidine incorporation was greater in the submandibular gland of BrdU-treated rats as compared to littermate controls. Isoproterenol stimulated thymidine incorporation into the submandibular glands of two-week-old rats. This stimulation was not observed in rats which received BrdU at age 7-9 days, prior to the administration of isoproterenol. Election microscopic observations, including a quantitative analysis of the frequency distribution of the various cell types in the terminal tubules and developing acini, indicated a retardation of acinar cell differentiation in the glands of BrdU-treated rats. In addition, there was an increase in the number and size of the secretory granules in the terminal tubule cells. BrdU treatment, however, caused no obvious pathologic alterations in the submandibular gland. Administration of isoproterenol for five days, beginning at five days of age, caused an apparent acceleration of the differentiation of acinar cells. In the glands of isoproterenol-treated rats the acinar cells were enlarged and were filled with homogeneous secretory granules. Pretreatment with BrdU partially inhibited the effects of isoproterenol on differentiation and hypertrophy of the submandibular gland. With the given dose of BrdU, approximately 5% of thymine was replaced by bromouracil in the DNA of the submandibular gland. Such a replacement would be consistent with a direct effect of BrdU on the cytodifferentiation in the submandibular gland. However, because of the severe retardation of growth of the BrdU-treated rats, indirect effects can not be excluded.", "contents": "The effects of 5-bromodeoxyuridine and isoproterenol on the postnatal differentiation of rat submandibular gland. The effects of 5-bromodeoxyuridine (BrdU) on the postnatal differentiation of rat submandibular gland and on the isoproterenol-induced changes of differentiation were studied. The rats were injected with BrdU for three consecutive days, beginning at two days of age. The total dose of BrdU was 0.9 mg/g body weight. BrdU caused a severe retardation of growth up to 15 days of age. A rapid growth of the animals between 15 and 22 days indicated a recovery from the effect of BrdU. The growth of the submandibular gland was retarded similarly with a corresponding decrease in DNA, RNA and protein content. Incorporation of tritiated thymidine into the submandibular gland was not altered in the BrdU-treated animals at one and three days after the last injection of the analog. At days 15 and 22 the rate of thymidine incorporation was greater in the submandibular gland of BrdU-treated rats as compared to littermate controls. Isoproterenol stimulated thymidine incorporation into the submandibular glands of two-week-old rats. This stimulation was not observed in rats which received BrdU at age 7-9 days, prior to the administration of isoproterenol. Election microscopic observations, including a quantitative analysis of the frequency distribution of the various cell types in the terminal tubules and developing acini, indicated a retardation of acinar cell differentiation in the glands of BrdU-treated rats. In addition, there was an increase in the number and size of the secretory granules in the terminal tubule cells. BrdU treatment, however, caused no obvious pathologic alterations in the submandibular gland. Administration of isoproterenol for five days, beginning at five days of age, caused an apparent acceleration of the differentiation of acinar cells. In the glands of isoproterenol-treated rats the acinar cells were enlarged and were filled with homogeneous secretory granules. Pretreatment with BrdU partially inhibited the effects of isoproterenol on differentiation and hypertrophy of the submandibular gland. With the given dose of BrdU, approximately 5% of thymine was replaced by bromouracil in the DNA of the submandibular gland. Such a replacement would be consistent with a direct effect of BrdU on the cytodifferentiation in the submandibular gland. However, because of the severe retardation of growth of the BrdU-treated rats, indirect effects can not be excluded.", "PMID": 970350}
{"id": "pubmed23n0032_7919", "title": "The systemic arterial pattern of the guinea pig: the pelvis and pelvic limb.", "content": "The systemic arterial pattern of 59 guinea pigs was examined by dissection, angiography, or corrosion casting. The blood supply to the pelvis and the pelvic limbs arises as two terminal branches of the aorta, the right and left common iliac arteries, and each of these divides into external and internal iliac arteries. Their pattern of distribution resembles a typical mammalian pattern. The external iliac artery passes throught the body wall to supply the pelvic limb and the internal iliac artery is distributed primarily to the pelvic region and its viscera.", "contents": "The systemic arterial pattern of the guinea pig: the pelvis and pelvic limb. The systemic arterial pattern of 59 guinea pigs was examined by dissection, angiography, or corrosion casting. The blood supply to the pelvis and the pelvic limbs arises as two terminal branches of the aorta, the right and left common iliac arteries, and each of these divides into external and internal iliac arteries. Their pattern of distribution resembles a typical mammalian pattern. The external iliac artery passes throught the body wall to supply the pelvic limb and the internal iliac artery is distributed primarily to the pelvic region and its viscera.", "PMID": 970351}
{"id": "pubmed23n0032_7920", "title": "Light and electron microscopic localization of acetylcholinesterase activity in the rat renal nerves.", "content": "Acetylcholinesterase activity is shown in the renal nerves of the rat with the technique of Karnovsky and Roots. By light microscopy, the acetylcholinesterase-positive nerves are seen in association with blood vessels, including the glomerular arterioles, and occasionally with renal tubules. By electron microscopy the precipitate appears extracellularly around axons and varicosities. DFP inhibits the deposition of precipitate. Previous demonstration by serial section electron microscopy in the rat revealed that all nerves around the glomerular arterioles contain small dense-cored vesicles characteristic of adrenergic nerves, indicating that the acetylcholinesterase-positive nerves demonstrated here are likely to be adrenergic nerves containing acetylcholinesterase.", "contents": "Light and electron microscopic localization of acetylcholinesterase activity in the rat renal nerves. Acetylcholinesterase activity is shown in the renal nerves of the rat with the technique of Karnovsky and Roots. By light microscopy, the acetylcholinesterase-positive nerves are seen in association with blood vessels, including the glomerular arterioles, and occasionally with renal tubules. By electron microscopy the precipitate appears extracellularly around axons and varicosities. DFP inhibits the deposition of precipitate. Previous demonstration by serial section electron microscopy in the rat revealed that all nerves around the glomerular arterioles contain small dense-cored vesicles characteristic of adrenergic nerves, indicating that the acetylcholinesterase-positive nerves demonstrated here are likely to be adrenergic nerves containing acetylcholinesterase.", "PMID": 970352}
{"id": "pubmed23n0032_7921", "title": "Melatonin induction of testicular recrudescence in hamsters and its subsequent inhibitory action on the antigonadotrophic influence of darkness on the pituitary-gonadal axis.", "content": "Exposure of adult male hamsters to light-dark (LD) cycles of 0:24 (in hours) for 12 weeks induced atrophy of the testes and accessory sex organs and a drop in immunoreactive pituitary and plasma prolactin levels. The subsequent exposure of these hamsters to LD 14:10 cycles caused regeneration of the sexual organs and an increase in pituitary LH and prolactin levels. Likewise, the weekly treatment of dark-exposed hamsters with subcutaneous melatonin-beeswax pellets led to regeneration of the peripheral reproductive organs and an increase in pituitary LH and prolactin levels. After gonadal regeneration, complete darkness failed to induce atrophy of the reproductive system indicating that either long photoperiods or melatonin treatment cause the pituitary-gonadal axis to be refractory to the inhibitory influence of darkness.", "contents": "Melatonin induction of testicular recrudescence in hamsters and its subsequent inhibitory action on the antigonadotrophic influence of darkness on the pituitary-gonadal axis. Exposure of adult male hamsters to light-dark (LD) cycles of 0:24 (in hours) for 12 weeks induced atrophy of the testes and accessory sex organs and a drop in immunoreactive pituitary and plasma prolactin levels. The subsequent exposure of these hamsters to LD 14:10 cycles caused regeneration of the sexual organs and an increase in pituitary LH and prolactin levels. Likewise, the weekly treatment of dark-exposed hamsters with subcutaneous melatonin-beeswax pellets led to regeneration of the peripheral reproductive organs and an increase in pituitary LH and prolactin levels. After gonadal regeneration, complete darkness failed to induce atrophy of the reproductive system indicating that either long photoperiods or melatonin treatment cause the pituitary-gonadal axis to be refractory to the inhibitory influence of darkness.", "PMID": 970353}
{"id": "pubmed23n0032_7922", "title": "Endothelial DNA synthesis in themicrovasculature of rat skin during the hair growth cycle.", "content": "Earlier studies of the vascular patterns around hair follicles revealed that actively growing follicles have an extensive microvasculature relative to that around quiescent follicles. In order to determine whether the establishment of the active perifollicular vascular pattern involves replication of endothelial and perivascular cells, we studied the 3H-thymidine labeling indices of these cells in autoradiographs of rat skin from regions containing either actively growing follicles or recently inactive follicles. In skin containing inactive follicles, the endothelial labeling index was 0.22%, a figure similar to those reported for the endothelium of small blood vessels in several other non-stimulated adult tissues. However, skin containing actively growing follicles displayed an endothelial labeling index of 2.35%. The labeling indices of perivascular cells paralleled those of the endothelial cells. Thus, endothelial and perivascular cells in vessels associated with actively growing follicles undergo significantly more DNA synthesis than those in vessels associated with inactive follicles. Replication of these cells apparently contributes to the extension of the microvascular network around growing follicles.", "contents": "Endothelial DNA synthesis in themicrovasculature of rat skin during the hair growth cycle. Earlier studies of the vascular patterns around hair follicles revealed that actively growing follicles have an extensive microvasculature relative to that around quiescent follicles. In order to determine whether the establishment of the active perifollicular vascular pattern involves replication of endothelial and perivascular cells, we studied the 3H-thymidine labeling indices of these cells in autoradiographs of rat skin from regions containing either actively growing follicles or recently inactive follicles. In skin containing inactive follicles, the endothelial labeling index was 0.22%, a figure similar to those reported for the endothelium of small blood vessels in several other non-stimulated adult tissues. However, skin containing actively growing follicles displayed an endothelial labeling index of 2.35%. The labeling indices of perivascular cells paralleled those of the endothelial cells. Thus, endothelial and perivascular cells in vessels associated with actively growing follicles undergo significantly more DNA synthesis than those in vessels associated with inactive follicles. Replication of these cells apparently contributes to the extension of the microvascular network around growing follicles.", "PMID": 970354}
{"id": "pubmed23n0032_7923", "title": "Relationship between the adrenal cortex and thymic involution in \"lethargic\" mutant mice.", "content": "To determine if the adrenal gland plays a role in the thymic involution which occurs spontaneously in \"lethargic\" mutant mice, three different studies were made. Morphological studies were made first to determine if there was an indication of histological changes in the adrenal glands. Next, serum levels of corticosterone were measured by radioimmunoassay. Finally, mice were unilaterally adrenalectomized to see if such treatment would improve various symptoms of \"lethargic\" mutants. Results of the studies showed that lipid granules in the cortical cells of \"lethargic\"mutants were greatly reduced in number during the time of spontaneous thymic involution, and the mutant mice had a significantly higher level of serum corticosterone than the normal controls. Mutant mice unilaterally adrenalectomized at 15 days of age showed a marked improvement in their condition and their mortality rate decreased. It is concluded that spontaneous thymic involution of \"lethargic\" mutants is probably associated with adrenocortical hypersecretion.", "contents": "Relationship between the adrenal cortex and thymic involution in \"lethargic\" mutant mice. To determine if the adrenal gland plays a role in the thymic involution which occurs spontaneously in \"lethargic\" mutant mice, three different studies were made. Morphological studies were made first to determine if there was an indication of histological changes in the adrenal glands. Next, serum levels of corticosterone were measured by radioimmunoassay. Finally, mice were unilaterally adrenalectomized to see if such treatment would improve various symptoms of \"lethargic\" mutants. Results of the studies showed that lipid granules in the cortical cells of \"lethargic\"mutants were greatly reduced in number during the time of spontaneous thymic involution, and the mutant mice had a significantly higher level of serum corticosterone than the normal controls. Mutant mice unilaterally adrenalectomized at 15 days of age showed a marked improvement in their condition and their mortality rate decreased. It is concluded that spontaneous thymic involution of \"lethargic\" mutants is probably associated with adrenocortical hypersecretion.", "PMID": 970355}
{"id": "pubmed23n0032_7924", "title": "A contribution to our knowledge of Leonurus L., i-mu-ts'ao, the Chinese motherwort.", "content": "This article deals with the ethnobotanical aspects of the Chinese motherwort. Since time immemorial the Chinese people have used various parts of motherwort to meet different physical needs. By the time a written language was developed and the medical uses were recorded. , motherwort was treated as an article of superior quality. At present, under the name of i-mu-ts'ao, the plant is used for improving bloodflow both by official physicians and herbal practitioners throughout the country as well as by villagers in isolated areas. According to Chinese classical literature on materia medica, the early uses were limited to the parts of the plant which met the most obvious needs of the prehistorical people in their struggle for existence-food and pain reliever. Evidently, in their search for food, the ancient people found that the four nutlets contained in the dry and spinose calyx of the Chinese motherwort resemble the seasame seed in size and oil content. They gathered them and used them for food in similar manner as with the sesame. Consequently, they discovered the good effects to the eyesight, the improvement of strength, and the uplift of spirit. These discoveries led to the use of the seed of the species as an eye medicine for improving the eyesight, and as a tonic for the increase of strength and the elevation of spirit. Contagious skin diseases caused serious problems for the ancient people. The use of the leafy shoot for a bath to release the discomfort of itches and shingles was also recorded in the 42-word first medicinal record of the species in the earliest known Chinese materia medica-the Shen-nung pen-ts'ao-ching. Translators of the Chinese classics have included the records of i-mu-ts'ao. According to my knowledge, these works are all partial translations with the selections of the medicinal properties and the omissions on the methods of preparation. They have the outline and abandon the details. Consequently most of them are not clear. In order to provide complete information on the discoveries of the ancient Chinese people on the uses of i-mu-ts'ao, all the records up to the end of the sixteenth century are organized and translated under the following headings: (1) ecological and morphological observations; (2) preparations; (3) physical and therapeutical properties; (4) special prescriptions for internal and external uses-including pills for pregnant women, for mothers post partum, as an emmenagogue, and as a corrective agent, condensed liquid, powder, fresh juice, baby bath and washes, poultices, charred shoots, gargles, drops and cakes; (5) other economic uses-including cosmetics and food; and (6) etymology. The distribution of i-mu-ts'ao is significant in photogeography and in the nomenclature of the species. I-mu-ts'ao was purposely introduced from South China to Linnaeus in Sweden before the publication of the Species Plantarum in 1753. Linnaeus planted the seed in the botanical garden of the University of Uppsala...", "contents": "A contribution to our knowledge of Leonurus L., i-mu-ts'ao, the Chinese motherwort. This article deals with the ethnobotanical aspects of the Chinese motherwort. Since time immemorial the Chinese people have used various parts of motherwort to meet different physical needs. By the time a written language was developed and the medical uses were recorded. , motherwort was treated as an article of superior quality. At present, under the name of i-mu-ts'ao, the plant is used for improving bloodflow both by official physicians and herbal practitioners throughout the country as well as by villagers in isolated areas. According to Chinese classical literature on materia medica, the early uses were limited to the parts of the plant which met the most obvious needs of the prehistorical people in their struggle for existence-food and pain reliever. Evidently, in their search for food, the ancient people found that the four nutlets contained in the dry and spinose calyx of the Chinese motherwort resemble the seasame seed in size and oil content. They gathered them and used them for food in similar manner as with the sesame. Consequently, they discovered the good effects to the eyesight, the improvement of strength, and the uplift of spirit. These discoveries led to the use of the seed of the species as an eye medicine for improving the eyesight, and as a tonic for the increase of strength and the elevation of spirit. Contagious skin diseases caused serious problems for the ancient people. The use of the leafy shoot for a bath to release the discomfort of itches and shingles was also recorded in the 42-word first medicinal record of the species in the earliest known Chinese materia medica-the Shen-nung pen-ts'ao-ching. Translators of the Chinese classics have included the records of i-mu-ts'ao. According to my knowledge, these works are all partial translations with the selections of the medicinal properties and the omissions on the methods of preparation. They have the outline and abandon the details. Consequently most of them are not clear. In order to provide complete information on the discoveries of the ancient Chinese people on the uses of i-mu-ts'ao, all the records up to the end of the sixteenth century are organized and translated under the following headings: (1) ecological and morphological observations; (2) preparations; (3) physical and therapeutical properties; (4) special prescriptions for internal and external uses-including pills for pregnant women, for mothers post partum, as an emmenagogue, and as a corrective agent, condensed liquid, powder, fresh juice, baby bath and washes, poultices, charred shoots, gargles, drops and cakes; (5) other economic uses-including cosmetics and food; and (6) etymology. The distribution of i-mu-ts'ao is significant in photogeography and in the nomenclature of the species. I-mu-ts'ao was purposely introduced from South China to Linnaeus in Sweden before the publication of the Species Plantarum in 1753. Linnaeus planted the seed in the botanical garden of the University of Uppsala...", "PMID": 970356}
{"id": "pubmed23n0032_7925", "title": "Extraction of hypotensive principles from seeds of Cassia tora.", "content": "Seeds of Cassia tora Linn. (Leguminosae) are known in Chinese medicinal herbal practice as Chueh-ming-tzu. Aqueous and methanol extracts from these seeds elicit hypotensive effects on anesthetized rats. Preliminary phytochemical studies show that the active hypotensive principles are derived from the kernel of the seed and consist of mainly glycosides.", "contents": "Extraction of hypotensive principles from seeds of Cassia tora. Seeds of Cassia tora Linn. (Leguminosae) are known in Chinese medicinal herbal practice as Chueh-ming-tzu. Aqueous and methanol extracts from these seeds elicit hypotensive effects on anesthetized rats. Preliminary phytochemical studies show that the active hypotensive principles are derived from the kernel of the seed and consist of mainly glycosides.", "PMID": 970357}
{"id": "pubmed23n0032_7926", "title": "A possible reflex mechanism of hypotensive action of extract from Cassia tora seeds.", "content": "The Chinese medicinal herb Chueh-ming-tzu, seeds of Cassia tora (Leguminosae) Linn., elicits hypotensive effects in anesthetized rats. Experimental results indicate that the hypotensive effect of the Cassia tora extract possibly involves a vagal reflex which reciprocally alters the vasomotor tone of the centrally emanating sympathetic nervous system. It is shown that the capacity of the Cassia tora extract to reduce blood pressure is significantly reduced in vagotomized rats and that hypotensive effects are greatly antagonized in rats whose sympathetic nervous systems are interrupted by transection of the spinal cord.", "contents": "A possible reflex mechanism of hypotensive action of extract from Cassia tora seeds. The Chinese medicinal herb Chueh-ming-tzu, seeds of Cassia tora (Leguminosae) Linn., elicits hypotensive effects in anesthetized rats. Experimental results indicate that the hypotensive effect of the Cassia tora extract possibly involves a vagal reflex which reciprocally alters the vasomotor tone of the centrally emanating sympathetic nervous system. It is shown that the capacity of the Cassia tora extract to reduce blood pressure is significantly reduced in vagotomized rats and that hypotensive effects are greatly antagonized in rats whose sympathetic nervous systems are interrupted by transection of the spinal cord.", "PMID": 970358}
{"id": "pubmed23n0032_7927", "title": "Induction of labor by acupuncture electro-stimulation.", "content": "Induction of labor by acupuncture electro-stimulation was carried out in 31 patients. This was successful in 21 cases. The pattern of uterine contractions induced in these patients was similar to that in normal labor. No serious fetal or maternal complications occurred. The delay between the commencement of stimulation and the onset of contractions suggests that a humoral mechanism may be involved.", "contents": "Induction of labor by acupuncture electro-stimulation. Induction of labor by acupuncture electro-stimulation was carried out in 31 patients. This was successful in 21 cases. The pattern of uterine contractions induced in these patients was similar to that in normal labor. No serious fetal or maternal complications occurred. The delay between the commencement of stimulation and the onset of contractions suggests that a humoral mechanism may be involved.", "PMID": 970359}
{"id": "pubmed23n0032_7928", "title": "Acupuncture for several functional disorders. Part I.", "content": "This is Part I of a study whose purpose is two-fold, that is, to arrive at a classification of diseases under investigation according to their responsiveness to acupuncture therapy, and to discuss and identify the most effective loci for the diseases investigated. In Part I of this study, over 500 patients afflicted with hyperthyroidism, trigeminal neuralgia or intercostal neuralgia were treated with acupuncture therapy. The results of the treatment, together with the selection of acupuncture loci and the techniques of needle manipulation are reported in detail. Over 80% of the patients were found to have made excellent improvement. The three diseases are therefore classified as diseases on which acupuncture therapy yields excellent results.", "contents": "Acupuncture for several functional disorders. Part I. This is Part I of a study whose purpose is two-fold, that is, to arrive at a classification of diseases under investigation according to their responsiveness to acupuncture therapy, and to discuss and identify the most effective loci for the diseases investigated. In Part I of this study, over 500 patients afflicted with hyperthyroidism, trigeminal neuralgia or intercostal neuralgia were treated with acupuncture therapy. The results of the treatment, together with the selection of acupuncture loci and the techniques of needle manipulation are reported in detail. Over 80% of the patients were found to have made excellent improvement. The three diseases are therefore classified as diseases on which acupuncture therapy yields excellent results.", "PMID": 970360}
{"id": "pubmed23n0032_7929", "title": "ECT and Acu-EST in the treatment of depression.", "content": "This paper presents three brief case histories from a three year study comparing electroconvulsive therapy (ECT) with acupuncture treatment in functional psychoses. Acupuncture effects were augmented by the simultaneous, non-painful electrical stimulation of eight acupuncture needles. The resulting treatment is called Acupuncture Electric Stimulation Therapy (Acu-EST). Each patient was utilized as her own control, treating exacerbations of depression alternately with ECT and Acu-EST. Acu-EST was not found to be a panacea and did not enable the discontinuation of antidepressant and neuroleptic medication. Individual Acu-EST treatments were often less effective than individual ECT treatments. However, series of Acu-EST did assist in producing significant remissions in depressive symptomatology. Because it did not produce the temporary disabling memory defects which occurred with ECT, Acu-EST was more easily adaptable to outpatient treatment.", "contents": "ECT and Acu-EST in the treatment of depression. This paper presents three brief case histories from a three year study comparing electroconvulsive therapy (ECT) with acupuncture treatment in functional psychoses. Acupuncture effects were augmented by the simultaneous, non-painful electrical stimulation of eight acupuncture needles. The resulting treatment is called Acupuncture Electric Stimulation Therapy (Acu-EST). Each patient was utilized as her own control, treating exacerbations of depression alternately with ECT and Acu-EST. Acu-EST was not found to be a panacea and did not enable the discontinuation of antidepressant and neuroleptic medication. Individual Acu-EST treatments were often less effective than individual ECT treatments. However, series of Acu-EST did assist in producing significant remissions in depressive symptomatology. Because it did not produce the temporary disabling memory defects which occurred with ECT, Acu-EST was more easily adaptable to outpatient treatment.", "PMID": 970361}
{"id": "pubmed23n0032_7930", "title": "A new technique for the insertion of acupuncture needles.", "content": "A new technique to insert acupuncture needles into the tissue without touching the needle with the fingers of the acupuncturist was developed by the authors. It is easy to perform and completely eliminates the problem of contamination. The bending of the needle is less frequent and the pain sensation of the patient is also minimized.", "contents": "A new technique for the insertion of acupuncture needles. A new technique to insert acupuncture needles into the tissue without touching the needle with the fingers of the acupuncturist was developed by the authors. It is easy to perform and completely eliminates the problem of contamination. The bending of the needle is less frequent and the pain sensation of the patient is also minimized.", "PMID": 970362}
{"id": "pubmed23n0032_7931", "title": "Medical education in China.", "content": "This article concerns the changes in Chinese medical education which have taken place since the Cultural Revolution, specifically the relationship between political ideology and actual practice. It synthesizes the documentation which appeared in a series of articles devoted to a public discussion on the direction and emphasis in medical and health work published in Renmin Ribao (People's Daily), Peking, from December 8, 1968 to November 4, 1975. The major themes of the public discussion are: (a) medical and health work serving the masses; (b) insistence on the \"correct\" revolutionary line; (c) combining theory with practice; (d) unity of traditional Chinese and Western medicine; (e) putting prevention first; and (f) emphasis on medical personnel retaining the characteristics of the working people. This is followed by a transcript prepared by the authors from a tape recording made during a visit to Zhongshan Medical College of Guangzhou (Canton) on November 5, 1974 describing the relationship between political ideology and actual practice in the field of contemporary Chinese medical education.", "contents": "Medical education in China. This article concerns the changes in Chinese medical education which have taken place since the Cultural Revolution, specifically the relationship between political ideology and actual practice. It synthesizes the documentation which appeared in a series of articles devoted to a public discussion on the direction and emphasis in medical and health work published in Renmin Ribao (People's Daily), Peking, from December 8, 1968 to November 4, 1975. The major themes of the public discussion are: (a) medical and health work serving the masses; (b) insistence on the \"correct\" revolutionary line; (c) combining theory with practice; (d) unity of traditional Chinese and Western medicine; (e) putting prevention first; and (f) emphasis on medical personnel retaining the characteristics of the working people. This is followed by a transcript prepared by the authors from a tape recording made during a visit to Zhongshan Medical College of Guangzhou (Canton) on November 5, 1974 describing the relationship between political ideology and actual practice in the field of contemporary Chinese medical education.", "PMID": 970363}
{"id": "pubmed23n0032_7932", "title": "The effects of 0.1 and 1.0 per cent erythrocytes and hemolysis on serum chemistry values.", "content": "Changes in serum chemistry values as a result of incomplete removal of erythrocytes and in vitro hemolysis during the preparative process have been studied. Two levels of contamination, corresponding to removal of 99% and 99.9% of the erythrocytes, were used to examine the effects of both hemolyzed and intact cells. Forty chemical procedures and methods were considered. Serum LDH values were most strongly affected by hemolyzed erythrocytes. Potassium, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase, and iron showed smaller but significant effects due to the presence of 1% hemolyzed cells, with lesser effects observed at the 0.1% level. The presence of non-hemolyzed cells at either level did not significantly alter chemistry results.", "contents": "The effects of 0.1 and 1.0 per cent erythrocytes and hemolysis on serum chemistry values. Changes in serum chemistry values as a result of incomplete removal of erythrocytes and in vitro hemolysis during the preparative process have been studied. Two levels of contamination, corresponding to removal of 99% and 99.9% of the erythrocytes, were used to examine the effects of both hemolyzed and intact cells. Forty chemical procedures and methods were considered. Serum LDH values were most strongly affected by hemolyzed erythrocytes. Potassium, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase, and iron showed smaller but significant effects due to the presence of 1% hemolyzed cells, with lesser effects observed at the 0.1% level. The presence of non-hemolyzed cells at either level did not significantly alter chemistry results.", "PMID": 970364}
{"id": "pubmed23n0032_7933", "title": "Effects of specimen evaporation on quality control.", "content": "Serum samples stored in a refrigerator and in ambient room air were studied for alteration of mean weight and mean concentration values. The storage period selected was four hours and the samples were variously stored exposed, refrigerated, and covered with a sample tray cover and by an airtight cover. Some storage procedures produced values more than 2 SD from the mean for many analytes. These findings were confirmed by a parallel study revealing a decrease of sample weight after the four-hour storage period. Storage at 4 C in sample cups covered by an airtight opaque shield alleviated the problem. Failure to treat external quality control sample pools in the same way as other samples will produce unexplained out-of-control results.", "contents": "Effects of specimen evaporation on quality control. Serum samples stored in a refrigerator and in ambient room air were studied for alteration of mean weight and mean concentration values. The storage period selected was four hours and the samples were variously stored exposed, refrigerated, and covered with a sample tray cover and by an airtight cover. Some storage procedures produced values more than 2 SD from the mean for many analytes. These findings were confirmed by a parallel study revealing a decrease of sample weight after the four-hour storage period. Storage at 4 C in sample cups covered by an airtight opaque shield alleviated the problem. Failure to treat external quality control sample pools in the same way as other samples will produce unexplained out-of-control results.", "PMID": 970365}
{"id": "pubmed23n0032_7934", "title": "Assessment of the serum separator tube as an intermediate storage device within the laboratory.", "content": "An integral blood-drawing-serum separator tube, (SST) is evaluated for use as an in-laboratory serum storage device. Sera stored in the SST were compared with paired controls that were specimens from the same subjects stored in closed culture tubes. Eighty-six serum chemistry determinations showed no clinically significant change as a result of 48 hours of storage in the original blood-drawing tubes. Storage of serum in this manner provides the laboratory with specimens for reanalysis or additional analyses while eliminating manipulative steps, including serum removal, tube relabeling, and intertube transfers, and reducing errors due to misidentification and/or contamination.", "contents": "Assessment of the serum separator tube as an intermediate storage device within the laboratory. An integral blood-drawing-serum separator tube, (SST) is evaluated for use as an in-laboratory serum storage device. Sera stored in the SST were compared with paired controls that were specimens from the same subjects stored in closed culture tubes. Eighty-six serum chemistry determinations showed no clinically significant change as a result of 48 hours of storage in the original blood-drawing tubes. Storage of serum in this manner provides the laboratory with specimens for reanalysis or additional analyses while eliminating manipulative steps, including serum removal, tube relabeling, and intertube transfers, and reducing errors due to misidentification and/or contamination.", "PMID": 970366}
{"id": "pubmed23n0032_7935", "title": "Sources of error in glucose determinations in neonatal blood by glucose oxidase methods, including Dextrostix.", "content": "An investigation was carried out to examine why a glucose oxidase-peroxidase-orthodianisidine method for plasma glucose, without protein precipitation, gave low results for neonatal blood. The magnitude of the difference between the results with and without protein precipitation was examined in a clinical neonatal series, and in sera to which bilirubin, hemolysate, pure hemoglobin, and uric acid had been added. Systematic linear inhibition was demonstrated with bilirubin, and the results suggested that high concentrations of hemolysate and uric acid could also interfere. Use of alkaline protein precipitants eliminated the inhibition. Dextrostix test results for neonatal blood are compared with results of conventional glucose analyses and possible sources of discrepancy examined.", "contents": "Sources of error in glucose determinations in neonatal blood by glucose oxidase methods, including Dextrostix. An investigation was carried out to examine why a glucose oxidase-peroxidase-orthodianisidine method for plasma glucose, without protein precipitation, gave low results for neonatal blood. The magnitude of the difference between the results with and without protein precipitation was examined in a clinical neonatal series, and in sera to which bilirubin, hemolysate, pure hemoglobin, and uric acid had been added. Systematic linear inhibition was demonstrated with bilirubin, and the results suggested that high concentrations of hemolysate and uric acid could also interfere. Use of alkaline protein precipitants eliminated the inhibition. Dextrostix test results for neonatal blood are compared with results of conventional glucose analyses and possible sources of discrepancy examined.", "PMID": 970367}
{"id": "pubmed23n0032_7936", "title": "New diagnostic use of bone marrow acid and alkaline phosphatase.", "content": "Prostatic acid phosphatase and alkaline phosphatase values in bone marrow were correlated with skeletal surveys and diagnoses during a six-month study. In cases of biopsy-proven adenocarcinoma of the prostate, bone marrow prostatic acid phosphatase was the most consistently abnormal value. Diagnoses other than prostatic cancer involving the bone marrow, e.g., myeloma and leukemias, were associated with elevated prostatic acid phosphatase and alkaline phosphatase values. In cases in which the bone marrow was not involved by metastasis, these values were normal. Bone marrow prostatic acid phosphatase assay was found to be a very good tool for detecting early osseous metastases from any site, including prostatic adenocarcinoma.", "contents": "New diagnostic use of bone marrow acid and alkaline phosphatase. Prostatic acid phosphatase and alkaline phosphatase values in bone marrow were correlated with skeletal surveys and diagnoses during a six-month study. In cases of biopsy-proven adenocarcinoma of the prostate, bone marrow prostatic acid phosphatase was the most consistently abnormal value. Diagnoses other than prostatic cancer involving the bone marrow, e.g., myeloma and leukemias, were associated with elevated prostatic acid phosphatase and alkaline phosphatase values. In cases in which the bone marrow was not involved by metastasis, these values were normal. Bone marrow prostatic acid phosphatase assay was found to be a very good tool for detecting early osseous metastases from any site, including prostatic adenocarcinoma.", "PMID": 970368}
{"id": "pubmed23n0032_7937", "title": "Hemangiopericytoma-like intranasal tumors. A clinicopathologic study of 23 cases.", "content": "The clinical, microscopic, and gross pathologic features of 23 cases of intranasal hemangiopericytoma-like tumors are reviewed and studied. When in the nasal cavity, these lesions often originated in a paranasal sinus and extended into the nasal cavity secondarily. They occurred most commonly in adults in the sixth and seventh decades of life; there was no significant sex predilection. Twenty-two of the 23 patients were Caucasian. These patients most commonly had symptoms of nasal obstruction and epistaxis. Clinically the lesions were generally thought to represent allergic polyps. Although appearing microscopically as non-differentiated spindle-cell neoplasms, these lesions showed little nuclear or cytoplasmic pleomorphism, minimal mitotic activity, and no necrosis or hemorrhage or other evidence of anaplasia found in malignant tumors. Follow-up data showed no evidence to suggest a malignant or biologically unpredictable lesion. Nineteen of 22 cases followed showed no recurrence regardless of the treatment; those that recurred did so locally. No metastasis or other form of aggressive behavior attributed to hemangiopericytomas in other anatomic locations was seen in this series. Another case, diagnosed as a malignant hemangiopericytoma of the nasal cavity, showed dissimilar and anaplastic histologic features. This case metastasized and is discussed, though not included in this study.", "contents": "Hemangiopericytoma-like intranasal tumors. A clinicopathologic study of 23 cases. The clinical, microscopic, and gross pathologic features of 23 cases of intranasal hemangiopericytoma-like tumors are reviewed and studied. When in the nasal cavity, these lesions often originated in a paranasal sinus and extended into the nasal cavity secondarily. They occurred most commonly in adults in the sixth and seventh decades of life; there was no significant sex predilection. Twenty-two of the 23 patients were Caucasian. These patients most commonly had symptoms of nasal obstruction and epistaxis. Clinically the lesions were generally thought to represent allergic polyps. Although appearing microscopically as non-differentiated spindle-cell neoplasms, these lesions showed little nuclear or cytoplasmic pleomorphism, minimal mitotic activity, and no necrosis or hemorrhage or other evidence of anaplasia found in malignant tumors. Follow-up data showed no evidence to suggest a malignant or biologically unpredictable lesion. Nineteen of 22 cases followed showed no recurrence regardless of the treatment; those that recurred did so locally. No metastasis or other form of aggressive behavior attributed to hemangiopericytomas in other anatomic locations was seen in this series. Another case, diagnosed as a malignant hemangiopericytoma of the nasal cavity, showed dissimilar and anaplastic histologic features. This case metastasized and is discussed, though not included in this study.", "PMID": 970369}
{"id": "pubmed23n0032_7938", "title": "Hepatic lesions of central pericellular fibrosis in morbid obesity, and after jejunoileal bypass.", "content": "Histologic findings in liver biopsy specimens obtained from 88 patients before and one and two years after end-to-end jejunoileal bypass are compared. In addition to the expected fatty changes, mild changes of centrilobular, pericellular fibrosis were present in the initial biopsies in 8.6%; a year later they had become apparent in 46%. Portal-central bridging developed in 6.8%, and early micronodular cirrhosis in 3.4%--always in those with central pericellular fibrosis. Electron-microscopic study of pre-bypass liver biopsies from eight addtional patients showed collagen and electron-dense material resembling basement membranes within the spaces of Disse in seven, although only four had light-microscopic evidence of minimal central pericellular fibrosis. The existence of these light- and electron- microscopic changes before jejunoileal bypass suggests that there is a lesion in morbid obesity that may be exacerbated during the first year after operation.", "contents": "Hepatic lesions of central pericellular fibrosis in morbid obesity, and after jejunoileal bypass. Histologic findings in liver biopsy specimens obtained from 88 patients before and one and two years after end-to-end jejunoileal bypass are compared. In addition to the expected fatty changes, mild changes of centrilobular, pericellular fibrosis were present in the initial biopsies in 8.6%; a year later they had become apparent in 46%. Portal-central bridging developed in 6.8%, and early micronodular cirrhosis in 3.4%--always in those with central pericellular fibrosis. Electron-microscopic study of pre-bypass liver biopsies from eight addtional patients showed collagen and electron-dense material resembling basement membranes within the spaces of Disse in seven, although only four had light-microscopic evidence of minimal central pericellular fibrosis. The existence of these light- and electron- microscopic changes before jejunoileal bypass suggests that there is a lesion in morbid obesity that may be exacerbated during the first year after operation.", "PMID": 970370}
{"id": "pubmed23n0032_7939", "title": "Rhabdomyosarcoma in children. A clinicopathologic study of 35 patients.", "content": "Thirty-five patients with rhabdomyosarcoma were treated at the Children's Hospital Medical Center from 1953 to 1973. Factors important in diagnosis and prognosis included age, sex, site, stage of tumor, and specific pathologic type. Effects that surgery, radiation, and chemotherapy had on survivals were also analyzed. Survivals ranged from 2 to 20 years. The rates were 86% for Stage I disease; 40% for Stage II disease, and 0 for the more advanced lesions. A useful prognostic indicator was found in pathologic subgroupings. Survivals for the myxoid spindle-cell variant were 80% for all stages. For the partial maturation series with moderately well-differentiated rhabdomyoblasts, survival was 10% for all stages. For a combination of the above two types, survival was 40% for all stages. The alveolar types had no survivors, although these patients seemed to live longer with their disease.", "contents": "Rhabdomyosarcoma in children. A clinicopathologic study of 35 patients. Thirty-five patients with rhabdomyosarcoma were treated at the Children's Hospital Medical Center from 1953 to 1973. Factors important in diagnosis and prognosis included age, sex, site, stage of tumor, and specific pathologic type. Effects that surgery, radiation, and chemotherapy had on survivals were also analyzed. Survivals ranged from 2 to 20 years. The rates were 86% for Stage I disease; 40% for Stage II disease, and 0 for the more advanced lesions. A useful prognostic indicator was found in pathologic subgroupings. Survivals for the myxoid spindle-cell variant were 80% for all stages. For the partial maturation series with moderately well-differentiated rhabdomyoblasts, survival was 10% for all stages. For a combination of the above two types, survival was 40% for all stages. The alveolar types had no survivors, although these patients seemed to live longer with their disease.", "PMID": 970371}
{"id": "pubmed23n0032_7940", "title": "The adrenal cortex in cystic fibrosis of the pancreas.", "content": "The morphologies of the adrenal cortical zones of 25 children with cystic fibrosis and 24 control patients are presented. A technic for objective measurement of these zones is described. Hyperplasia of the zona glomerulosa in the children with cystic fibrosis is significant and may be related to chronic loss of salt in the sweat or demands placed on renal salt-conserving mechanisms.", "contents": "The adrenal cortex in cystic fibrosis of the pancreas. The morphologies of the adrenal cortical zones of 25 children with cystic fibrosis and 24 control patients are presented. A technic for objective measurement of these zones is described. Hyperplasia of the zona glomerulosa in the children with cystic fibrosis is significant and may be related to chronic loss of salt in the sweat or demands placed on renal salt-conserving mechanisms.", "PMID": 970372}
{"id": "pubmed23n0032_7941", "title": "Small-cell carcinoma of the male mammary gland. A tumor resembling infiltrating lobular carcinoma.", "content": "The cases of two patients with infiltrating lobular carcinoma of the male mammary gland are discussed. They represent two of a total of 16 male patients with mammary carcinomas examined at the Medical College of Virginia between 1950 and 1975. Two previous similar cases have been reported in the literature as \"small-cell\" carcinoma. In one of the current cases there is unexplained benign lobular proliferation with atypical features suggesting lobular origin of the infiltrating tumor.", "contents": "Small-cell carcinoma of the male mammary gland. A tumor resembling infiltrating lobular carcinoma. The cases of two patients with infiltrating lobular carcinoma of the male mammary gland are discussed. They represent two of a total of 16 male patients with mammary carcinomas examined at the Medical College of Virginia between 1950 and 1975. Two previous similar cases have been reported in the literature as \"small-cell\" carcinoma. In one of the current cases there is unexplained benign lobular proliferation with atypical features suggesting lobular origin of the infiltrating tumor.", "PMID": 970373}
{"id": "pubmed23n0032_7942", "title": "Probable acanthamoeba meningoencephalitis in a Korean child.", "content": "A 5-year-old Korean boy developed multiple subcutaneous, nontuberculous granulomas and died with meningoencephalitis. Autopsy disclosed amebic granulomas in subcutaneous tissue, the left adrenal gland, and the pancreas, with more acute inflammatory lesions in the liver, kidney, and brain. The causative organism is believed to be an Acanthamoeba sp.", "contents": "Probable acanthamoeba meningoencephalitis in a Korean child. A 5-year-old Korean boy developed multiple subcutaneous, nontuberculous granulomas and died with meningoencephalitis. Autopsy disclosed amebic granulomas in subcutaneous tissue, the left adrenal gland, and the pancreas, with more acute inflammatory lesions in the liver, kidney, and brain. The causative organism is believed to be an Acanthamoeba sp.", "PMID": 970374}
{"id": "pubmed23n0032_7943", "title": "Laboratory diagnosis of botulism complicated by pyridostigmine treatment of the patient. A method for selectively removing interfering substances from clinical specimens.", "content": "In August 1974, a case of botulism occurred; home-canned potatoes and peas containing Clostridium botulinum, type A, were strongly incriminated as the vehicle of transmission. C. botulinum, type A, was isolated from a stool specimen of the patient, but the mouse neutralization test for botulinal toxin could not be completed because the stool extract contained a highly toxic, heat-stable substance that rapidly killed mice. Historical and laboratory evidence indicated that the substance was pyridostigmine bromide, a low-molecular-weight drug with which the patient had been treated after her disease was misdiagnosed as myasthenia gravis. A generally applicable method employing dialysis by which toxic SUBSTANCED of low molecular weigth could be selectively removed from specimens without diminishing the potency of botulinal toxin contained in them was developed. Dialysis rendered a pyridostigmine solution, a stool extract from the patient with botulism, and a stool extract from a person taking pyridostigmine virtually nontoxic to mice. Dialysis did not significantly alter the toxicity to mice of crude botulinal toxin; it selectively eliminated all or almost all pyridostigmine toxicity from a pyridostigmine-botulinal toxin mixture without altering the toxicity of the botulinal toxin.", "contents": "Laboratory diagnosis of botulism complicated by pyridostigmine treatment of the patient. A method for selectively removing interfering substances from clinical specimens. In August 1974, a case of botulism occurred; home-canned potatoes and peas containing Clostridium botulinum, type A, were strongly incriminated as the vehicle of transmission. C. botulinum, type A, was isolated from a stool specimen of the patient, but the mouse neutralization test for botulinal toxin could not be completed because the stool extract contained a highly toxic, heat-stable substance that rapidly killed mice. Historical and laboratory evidence indicated that the substance was pyridostigmine bromide, a low-molecular-weight drug with which the patient had been treated after her disease was misdiagnosed as myasthenia gravis. A generally applicable method employing dialysis by which toxic SUBSTANCED of low molecular weigth could be selectively removed from specimens without diminishing the potency of botulinal toxin contained in them was developed. Dialysis rendered a pyridostigmine solution, a stool extract from the patient with botulism, and a stool extract from a person taking pyridostigmine virtually nontoxic to mice. Dialysis did not significantly alter the toxicity to mice of crude botulinal toxin; it selectively eliminated all or almost all pyridostigmine toxicity from a pyridostigmine-botulinal toxin mixture without altering the toxicity of the botulinal toxin.", "PMID": 970375}
{"id": "pubmed23n0032_7944", "title": "Mapping of several abnormal hemoglobins by horizontal polyacrylamide gel isoelectric focusing.", "content": "The relative mobilities of serveral human hemoglobin variants were studied by use of an isoelectric focusing method in a thin-layer horizontal polyacrylamide gel. The technic is described in detail and a preliminary mapping of these variants is presented. It appears that thin-layer gel isoelectric focusing is a method that is rapid, highly reproducible and easily applicable in laboratories concerned with the study of hemoglobinopathies.", "contents": "Mapping of several abnormal hemoglobins by horizontal polyacrylamide gel isoelectric focusing. The relative mobilities of serveral human hemoglobin variants were studied by use of an isoelectric focusing method in a thin-layer horizontal polyacrylamide gel. The technic is described in detail and a preliminary mapping of these variants is presented. It appears that thin-layer gel isoelectric focusing is a method that is rapid, highly reproducible and easily applicable in laboratories concerned with the study of hemoglobinopathies.", "PMID": 970376}
{"id": "pubmed23n0032_7945", "title": "Effects of patient racial attribution on black clinicians' inferences.", "content": "This study was done to determine the impact of patient racial attribution on black practitioners' clinical judgments. Fifty-five professionals indicated their clinical reactions to a bogus case description of a sexually maladjusted male patient identified as black or white and also completed a traditional social beliefs scale. Relatively untraditional clinicians evaluated the black-designated patient more favorably than the white-designated patient. The less traditional practitioners also evaluated the black-designated patient more favorably than did the traditional clinicians. Effects of patient race were found for both clinical impressions and treatment decisions and were not attenuated in the more highly experienced subsample. Implications and limitations of the findings are discussed.", "contents": "Effects of patient racial attribution on black clinicians' inferences. This study was done to determine the impact of patient racial attribution on black practitioners' clinical judgments. Fifty-five professionals indicated their clinical reactions to a bogus case description of a sexually maladjusted male patient identified as black or white and also completed a traditional social beliefs scale. Relatively untraditional clinicians evaluated the black-designated patient more favorably than the white-designated patient. The less traditional practitioners also evaluated the black-designated patient more favorably than did the traditional clinicians. Effects of patient race were found for both clinical impressions and treatment decisions and were not attenuated in the more highly experienced subsample. Implications and limitations of the findings are discussed.", "PMID": 970377}
{"id": "pubmed23n0032_7946", "title": "Untrained paraprofessionals' verbal helping behavior. Description and implications for training.", "content": "The verbal behavior of untrained paraprofessionals in simulated initial helping interactions was studied. Taped interviews conducted by 131 helpers-in-training were evaluated on the Helping Skills Verbal Response System, a category system consisting of continuing, leading, and self-referent responses. Helpers were found to vary considerably in number and types of responses given. Leading responses were twice as frequent as continuing responses. The most often used response was the closed question. Few responses focusing on helpees' feelings were given. The implications for training were discussed in terms of the facilitative role of helpers in initial helping interactions.", "contents": "Untrained paraprofessionals' verbal helping behavior. Description and implications for training. The verbal behavior of untrained paraprofessionals in simulated initial helping interactions was studied. Taped interviews conducted by 131 helpers-in-training were evaluated on the Helping Skills Verbal Response System, a category system consisting of continuing, leading, and self-referent responses. Helpers were found to vary considerably in number and types of responses given. Leading responses were twice as frequent as continuing responses. The most often used response was the closed question. Few responses focusing on helpees' feelings were given. The implications for training were discussed in terms of the facilitative role of helpers in initial helping interactions.", "PMID": 970378}
{"id": "pubmed23n0032_7947", "title": "The development and evaluation of a community mental health education program for seminarians.", "content": "Because the clergy stands in an ideal position to serve the mental health needs of the community, seminaries have, for some time, included courses in pastoral counseling and related skills. While valuable, these courses tend to focus on the traditional clinical relationship excluding the broader role of community interventionist. Viewing this need, a course was developed to train seminarians in principles and practices of community mental health. The 28 students were compared to 24 controls at the beginning and end of the term. Evaluative instruments were the Community Mental Health Ideology Scale, two scales adapted from the Theological School Inventory, a self-evaluative competence scale, and a role evaluation scale. Data within a 2 X 2 X 2 design were subjected to an analysis of variance. Experimentals were found to exceed controls on the Community Mental Health Ideology Scale (p less than or equal to .001), the competence scale (p less than or equal to .01), and role evaluation scale (p less than or equal to .01).", "contents": "The development and evaluation of a community mental health education program for seminarians. Because the clergy stands in an ideal position to serve the mental health needs of the community, seminaries have, for some time, included courses in pastoral counseling and related skills. While valuable, these courses tend to focus on the traditional clinical relationship excluding the broader role of community interventionist. Viewing this need, a course was developed to train seminarians in principles and practices of community mental health. The 28 students were compared to 24 controls at the beginning and end of the term. Evaluative instruments were the Community Mental Health Ideology Scale, two scales adapted from the Theological School Inventory, a self-evaluative competence scale, and a role evaluation scale. Data within a 2 X 2 X 2 design were subjected to an analysis of variance. Experimentals were found to exceed controls on the Community Mental Health Ideology Scale (p less than or equal to .001), the competence scale (p less than or equal to .01), and role evaluation scale (p less than or equal to .01).", "PMID": 970379}
{"id": "pubmed23n0032_7948", "title": "Evaluation of crisis intervention services with anonymous clients.", "content": "A technique is introduced for using intake data to evaluate crisis intervention services in which client anonymity prevents the usual follow-up procedures. The technique is based on the assumptions (a) that the rate of reuse of the service indicates the effectiveness of the service, and (b) that counselors' estimates of their effectiveness provide some indication of the adequacy of their training. The study was conducted at a 24-hour metropolitan hotline. In addition to obtaining the usual intake information, counselors asked callers how often they had used the hotline previously and then rated their own effectiveness in aiding the callers. Analyses show the relative effectiveness of the hotline with various types of callers and problems and suggest areas where additional counselor training is desirable.", "contents": "Evaluation of crisis intervention services with anonymous clients. A technique is introduced for using intake data to evaluate crisis intervention services in which client anonymity prevents the usual follow-up procedures. The technique is based on the assumptions (a) that the rate of reuse of the service indicates the effectiveness of the service, and (b) that counselors' estimates of their effectiveness provide some indication of the adequacy of their training. The study was conducted at a 24-hour metropolitan hotline. In addition to obtaining the usual intake information, counselors asked callers how often they had used the hotline previously and then rated their own effectiveness in aiding the callers. Analyses show the relative effectiveness of the hotline with various types of callers and problems and suggest areas where additional counselor training is desirable.", "PMID": 970380}
{"id": "pubmed23n0032_7949", "title": "Evaluating the crisis service of a community mental health center.", "content": "This paper describes the use of an empirically based evaluation system for the Crisis Service of a comprehensive community mental health center. Through cooperative agreements with community agencies, jailings of behaviorally disturbed individuals have decreased in number and duration. Evaluations have also measured response time and success of recommendations made by workers. The authors describe how the Crisis Service's  evaluation techniques reflect the Center's commitment to accountability, a behavioral approach, and community orientation. Data concerning evaluation of the service are presented.", "contents": "Evaluating the crisis service of a community mental health center. This paper describes the use of an empirically based evaluation system for the Crisis Service of a comprehensive community mental health center. Through cooperative agreements with community agencies, jailings of behaviorally disturbed individuals have decreased in number and duration. Evaluations have also measured response time and success of recommendations made by workers. The authors describe how the Crisis Service's  evaluation techniques reflect the Center's commitment to accountability, a behavioral approach, and community orientation. Data concerning evaluation of the service are presented.", "PMID": 970381}
{"id": "pubmed23n0032_7950", "title": "Hyperamylasemia in heroin addicts. Characterization by isoamylase analysis.", "content": "Hyperamylasemia was noted in 17 (19%) of a group of 91 hospitalized heroin addicts. Thirteen of the 17 were in acute respiratory distress (12 with so-called \"heroin lung\" syndrome and one with status asthmaticus). Isoamylase analysis in the hyperamylasemic patients demonstrated S-type isoamylase dominance in 15, P-type isoamylase dominance in one and essentially equivalent P- and S-type isoamylase elevations in one. It would appear from these data that the hyperamylasemia after heroin in most persons addicted to the use of this drug arises from the sources other than the pancreas. Changes in the lungs occurring in association with heroin addiction seem to have an important role among the possible contributory factors.", "contents": "Hyperamylasemia in heroin addicts. Characterization by isoamylase analysis. Hyperamylasemia was noted in 17 (19%) of a group of 91 hospitalized heroin addicts. Thirteen of the 17 were in acute respiratory distress (12 with so-called \"heroin lung\" syndrome and one with status asthmaticus). Isoamylase analysis in the hyperamylasemic patients demonstrated S-type isoamylase dominance in 15, P-type isoamylase dominance in one and essentially equivalent P- and S-type isoamylase elevations in one. It would appear from these data that the hyperamylasemia after heroin in most persons addicted to the use of this drug arises from the sources other than the pancreas. Changes in the lungs occurring in association with heroin addiction seem to have an important role among the possible contributory factors.", "PMID": 970382}
{"id": "pubmed23n0032_7951", "title": "Colonscopic excision of sessile polyps.", "content": "Most benign sessile colon polyps can be removed colonoscopically from all reaches of the colon, thus avoiding laparotomy previously required for most of these lesions. Two hundred and fifty colonoscopy procedures were reviewed, revealing 87 sessile colon polyps ranging from 0.5-6.0 cm. in size. Sixty-eight of 87 lesions were excised from 51 patients with just three lesions found to be malignant (invasive adenocarcinoma). Sixteen lesions were felt to be unsuitable for safe colonscopic excision and were, therefore, removed by laparotomy (in 12 patients) and six lesions found to be malignant. Three sessile lesions in this consecutive series were not yet removed at the time of this writing. Thorough bowel preparation and evaluation for other possible lesions are important components of this procedure, which yielded other polyps or cancers in 24 of 66 patients. While most sessile lesions less than 2 cm. in size can be excised endoscopically, certain larger benign sessile lesions can also be removed using piece-meal snare excision technics and radiowave electrocautery (principle of less heat penetration and tissue destruction). Seventy-nine per cent of all sessile colon polyps were excised colonoscopically in this series making abdominal surgery now unnecessary for most colon polyps. Laparotomy is necessary for certain larger benign lesions and all sessile lesions containing invasive cancer.", "contents": "Colonscopic excision of sessile polyps. Most benign sessile colon polyps can be removed colonoscopically from all reaches of the colon, thus avoiding laparotomy previously required for most of these lesions. Two hundred and fifty colonoscopy procedures were reviewed, revealing 87 sessile colon polyps ranging from 0.5-6.0 cm. in size. Sixty-eight of 87 lesions were excised from 51 patients with just three lesions found to be malignant (invasive adenocarcinoma). Sixteen lesions were felt to be unsuitable for safe colonscopic excision and were, therefore, removed by laparotomy (in 12 patients) and six lesions found to be malignant. Three sessile lesions in this consecutive series were not yet removed at the time of this writing. Thorough bowel preparation and evaluation for other possible lesions are important components of this procedure, which yielded other polyps or cancers in 24 of 66 patients. While most sessile lesions less than 2 cm. in size can be excised endoscopically, certain larger benign sessile lesions can also be removed using piece-meal snare excision technics and radiowave electrocautery (principle of less heat penetration and tissue destruction). Seventy-nine per cent of all sessile colon polyps were excised colonoscopically in this series making abdominal surgery now unnecessary for most colon polyps. Laparotomy is necessary for certain larger benign lesions and all sessile lesions containing invasive cancer.", "PMID": 970383}
{"id": "pubmed23n0032_7952", "title": "Tuberculous peritonitis. A case report and review.", "content": "A 32-year old Dominican woman with signs and symptoms of an acute abdomen, was found to have tuberculous peritonitis. Our experience with this patient is presented along with a review of the literature. Population shifts may bring about an increased number of cases in this country. Awareness of this entity will avoid unnecessary diagnostic errors and laparotomy. The surgeon should also be versed in the total management and be prepared to handle the surgical complications of the disease.", "contents": "Tuberculous peritonitis. A case report and review. A 32-year old Dominican woman with signs and symptoms of an acute abdomen, was found to have tuberculous peritonitis. Our experience with this patient is presented along with a review of the literature. Population shifts may bring about an increased number of cases in this country. Awareness of this entity will avoid unnecessary diagnostic errors and laparotomy. The surgeon should also be versed in the total management and be prepared to handle the surgical complications of the disease.", "PMID": 970384}
{"id": "pubmed23n0032_7953", "title": "Benign lymphoid hyperplasia of the stomach mimicking gastric malignancy.", "content": "A case of benign lymphoid hyperplasia of the stomach misdiagnosed as ulcerating gastric carcinoma is presented. The similarities in the clinical, roentgenologic and and gastroscopic features as well as in the gross appearance of the two lesions are described and the features which help differentiation are highlighted. The pathologic picture suggested that the lesion possibly arose as a peptic ulcer and the clinical history further supports such a possibility.", "contents": "Benign lymphoid hyperplasia of the stomach mimicking gastric malignancy. A case of benign lymphoid hyperplasia of the stomach misdiagnosed as ulcerating gastric carcinoma is presented. The similarities in the clinical, roentgenologic and and gastroscopic features as well as in the gross appearance of the two lesions are described and the features which help differentiation are highlighted. The pathologic picture suggested that the lesion possibly arose as a peptic ulcer and the clinical history further supports such a possibility.", "PMID": 970385}
{"id": "pubmed23n0032_7954", "title": "Optimum dose of histamine for maximal acid output in underweight subjects.", "content": "The effects of increasing doses of histamine acid phosphate on the gastric secretion of acid has been studied in a randomized fashion in five normal control and 15 duodenal ulcer subjects. The dose of histamine acid phosphate used in each individual was 40, 70, 76 and 80 mug/kg. and 3.2 mg. of histamine. In 12 of 20 subjects the highest mean +/- S.D. acid output was observed after a dose of 76 mug./kg. body weight. When the acid output was expressed as the percentage of the highest response with different doses of HAP, the response was maximum with 76 mug. kg. dose. Further statistical analysis by rank totals and X2r by Friedman's two-way analysis by ranks also revealed highest acid output after a 76 mug./kg. dose of HAP. These findings suggest that 76 mug./kg. is the optimum dose for maximal acid output in underweight subjects. Only one patient showed highest acid output after a 3.2 mg. dose of histamine. The mean +/- S.D. acid output after 76 mug./kg. was higher than that after a 3.2 mg. dose of histamine. Hence, for estimating maximal acid output, a weight related dose and not a fixed dose is recommended. Further, it was shown that there was significant correlation between PAO and MAO after various doses of histamine in both the groups. Hence, for clinical purposes, estimation of one hourly poststimulatory maximal acid output after a dose of 76 mug./kg. body weight of histamine acid phosphate is recommended for underweight subjects.", "contents": "Optimum dose of histamine for maximal acid output in underweight subjects. The effects of increasing doses of histamine acid phosphate on the gastric secretion of acid has been studied in a randomized fashion in five normal control and 15 duodenal ulcer subjects. The dose of histamine acid phosphate used in each individual was 40, 70, 76 and 80 mug/kg. and 3.2 mg. of histamine. In 12 of 20 subjects the highest mean +/- S.D. acid output was observed after a dose of 76 mug./kg. body weight. When the acid output was expressed as the percentage of the highest response with different doses of HAP, the response was maximum with 76 mug. kg. dose. Further statistical analysis by rank totals and X2r by Friedman's two-way analysis by ranks also revealed highest acid output after a 76 mug./kg. dose of HAP. These findings suggest that 76 mug./kg. is the optimum dose for maximal acid output in underweight subjects. Only one patient showed highest acid output after a 3.2 mg. dose of histamine. The mean +/- S.D. acid output after 76 mug./kg. was higher than that after a 3.2 mg. dose of histamine. Hence, for estimating maximal acid output, a weight related dose and not a fixed dose is recommended. Further, it was shown that there was significant correlation between PAO and MAO after various doses of histamine in both the groups. Hence, for clinical purposes, estimation of one hourly poststimulatory maximal acid output after a dose of 76 mug./kg. body weight of histamine acid phosphate is recommended for underweight subjects.", "PMID": 970386}
{"id": "pubmed23n0032_7955", "title": "Afferent loop syndrome: a different picture.", "content": "A patient who had a 50% gastrectomy with a Billroth II gastrojejunostomy one and a half years previously, complained of recent severe weakness as the only symptom was found to have an iron deficiency anemia with a periumbilical mass. A gastrointestinal series showed a soft tissue density in the epigastric area which, by ultrasonography, was found to be fluid-containing. Laparotomy revealed obstruction of the afferent loop caused by a marginal ulcer.", "contents": "Afferent loop syndrome: a different picture. A patient who had a 50% gastrectomy with a Billroth II gastrojejunostomy one and a half years previously, complained of recent severe weakness as the only symptom was found to have an iron deficiency anemia with a periumbilical mass. A gastrointestinal series showed a soft tissue density in the epigastric area which, by ultrasonography, was found to be fluid-containing. Laparotomy revealed obstruction of the afferent loop caused by a marginal ulcer.", "PMID": 970389}
{"id": "pubmed23n0032_7956", "title": "Carcinoma of the gallbladder: a diagnosis aided by endoscopic retrograde and percutaneous hepatic cholangiography.", "content": "In a patient with obstructive jaundice, extrinsic compression of the common bile duct (Mirizzi syndrome) due to squamous cell carcinoma of the gallbladder was documented with the combination of endoscopic retrograde cholangiography and pancreatography (ERCP) and percutaneous transhepatic cholangiography (PTC).", "contents": "Carcinoma of the gallbladder: a diagnosis aided by endoscopic retrograde and percutaneous hepatic cholangiography. In a patient with obstructive jaundice, extrinsic compression of the common bile duct (Mirizzi syndrome) due to squamous cell carcinoma of the gallbladder was documented with the combination of endoscopic retrograde cholangiography and pancreatography (ERCP) and percutaneous transhepatic cholangiography (PTC).", "PMID": 970390}
{"id": "pubmed23n0032_7957", "title": "Chymotrypsin output in the stools in pancreatic and other diseases.", "content": "Chymotrypsin output in the stools (u./24 hr.) has been calculated from analysis of single stool samples, using cuprous isothiocyanate as a continuous marker. The values thus calculated agreed favorably with those calculated from three-day stool collections. In 45 controls, with and without steatorrhea, chymotrypsin output was above 10,000 u./24 hr. In six patients with pancreatic insufficiency the values were considerably lower. In 10 patients with pancreatic disease without insufficiency the values were in the normal range. The method was found very useful in the diagnosis of pancreatic insufficiency. It was not sensitive enough to detect lesser degrees of pancreatic damage.", "contents": "Chymotrypsin output in the stools in pancreatic and other diseases. Chymotrypsin output in the stools (u./24 hr.) has been calculated from analysis of single stool samples, using cuprous isothiocyanate as a continuous marker. The values thus calculated agreed favorably with those calculated from three-day stool collections. In 45 controls, with and without steatorrhea, chymotrypsin output was above 10,000 u./24 hr. In six patients with pancreatic insufficiency the values were considerably lower. In 10 patients with pancreatic disease without insufficiency the values were in the normal range. The method was found very useful in the diagnosis of pancreatic insufficiency. It was not sensitive enough to detect lesser degrees of pancreatic damage.", "PMID": 970391}
{"id": "pubmed23n0032_7958", "title": "Studies on factors affecting healing of gastric ulcer. A prospective, cooperative study in Japan.", "content": "To assess factors influencing the healing of gastric ulcer, a prospective and cooperative study was carried out in Osaka, Japan. Four hundred nineteen patients with single gastric ulcer were followed from May, 1971 to June, 1973. These patients were divided into two groups, \"healing\" and \"delayed healing\", on the basis of the criteria by the distribution analysis. By the multivariate analysis of 11 factors picked up, site, size of the ulcer and age were related to healing of gastric ulcer with the larger weight. These results suggest that ulcers of smaller lesions, ulcers of the gastric body, ulcers of the anterior wall or younger patients show faster healing in general.", "contents": "Studies on factors affecting healing of gastric ulcer. A prospective, cooperative study in Japan. To assess factors influencing the healing of gastric ulcer, a prospective and cooperative study was carried out in Osaka, Japan. Four hundred nineteen patients with single gastric ulcer were followed from May, 1971 to June, 1973. These patients were divided into two groups, \"healing\" and \"delayed healing\", on the basis of the criteria by the distribution analysis. By the multivariate analysis of 11 factors picked up, site, size of the ulcer and age were related to healing of gastric ulcer with the larger weight. These results suggest that ulcers of smaller lesions, ulcers of the gastric body, ulcers of the anterior wall or younger patients show faster healing in general.", "PMID": 970393}
{"id": "pubmed23n0032_7959", "title": "The microcirculatory correlator: a new instrument for the intraoperative evaluation of visceral circulatory integrity.", "content": "The microcirculatory correlator probe has been used on multiple occasions on the abdominal viscera and clear pulse-wave tracings have been obtained from all the intra-abdominal organs. There is a linear correlation between deflection of pulse-wave and intestine blood flow in an isolated loop of intestine under low flow conditions. The probe has been used in two circumstances to good clinical advantage.", "contents": "The microcirculatory correlator: a new instrument for the intraoperative evaluation of visceral circulatory integrity. The microcirculatory correlator probe has been used on multiple occasions on the abdominal viscera and clear pulse-wave tracings have been obtained from all the intra-abdominal organs. There is a linear correlation between deflection of pulse-wave and intestine blood flow in an isolated loop of intestine under low flow conditions. The probe has been used in two circumstances to good clinical advantage.", "PMID": 970395}
{"id": "pubmed23n0032_7960", "title": "Fine needle cholangiography in the diagnosis of persistent jaundice. A case report.", "content": "The fine needle technic utilizes a sleeveless, thin, flexible needle for transhepatic cholangiography. This needle can enter small or normal bile ducts in a high percentage of patients. The value of fine needle cholangiography is illustrated in a patient with a stenosis of the common hepatic duct and a left hepatodochalduodenal fistula which eluded diagnosis by all other methods. The use of this technic is described as an alternative procedure to endoscopic retrograde cholangiography when the latter is technically impossible or as an adjunct to intraoperative extrahepatic cholangiography.", "contents": "Fine needle cholangiography in the diagnosis of persistent jaundice. A case report. The fine needle technic utilizes a sleeveless, thin, flexible needle for transhepatic cholangiography. This needle can enter small or normal bile ducts in a high percentage of patients. The value of fine needle cholangiography is illustrated in a patient with a stenosis of the common hepatic duct and a left hepatodochalduodenal fistula which eluded diagnosis by all other methods. The use of this technic is described as an alternative procedure to endoscopic retrograde cholangiography when the latter is technically impossible or as an adjunct to intraoperative extrahepatic cholangiography.", "PMID": 970397}
{"id": "pubmed23n0032_7961", "title": "A rapid, manual test for amylase at 52C.", "content": "A simple modification of an alpha-amylase (E.C. 3.2.1.1.) procedure, using blue starch substrate, permits quantitative results after three minutes of incubation. The procedure, performed on blank, control, and unknown speciemns, takes advantage of the heat stability of the enzyme and provides immediate and reliable data for acutely ill patients. The performance characteristics of the test on serum and urine are described. The method is distinctly advantageous in prividing rapid results for serum amylase, urine amylase, and amylase clearance in patients with suspected pancreatitis.", "contents": "A rapid, manual test for amylase at 52C. A simple modification of an alpha-amylase (E.C. 3.2.1.1.) procedure, using blue starch substrate, permits quantitative results after three minutes of incubation. The procedure, performed on blank, control, and unknown speciemns, takes advantage of the heat stability of the enzyme and provides immediate and reliable data for acutely ill patients. The performance characteristics of the test on serum and urine are described. The method is distinctly advantageous in prividing rapid results for serum amylase, urine amylase, and amylase clearance in patients with suspected pancreatitis.", "PMID": 970406}
{"id": "pubmed23n0032_7962", "title": "Practical aspects of quality control for radioimmunoassays.", "content": "Mechanisms for the quality control of radioimmunoassays are described. They are useful in monitoring the possible sources of error which cause medically invalid results: errors in the radioimmunoassay system itself, in the test environment, in the instrumentation, and in the specimen quality. The application of a total control program permits early detection of malfunctions and can be used for the logical assessment of probable causes and solutions.", "contents": "Practical aspects of quality control for radioimmunoassays. Mechanisms for the quality control of radioimmunoassays are described. They are useful in monitoring the possible sources of error which cause medically invalid results: errors in the radioimmunoassay system itself, in the test environment, in the instrumentation, and in the specimen quality. The application of a total control program permits early detection of malfunctions and can be used for the logical assessment of probable causes and solutions.", "PMID": 970407}
{"id": "pubmed23n0032_7963", "title": "Automatic blood film preparation by rheologically controlled spinning.", "content": "During the past six years at the Biomedical Technical Center of Corning Glass Works, extensive studies on blood film preparation have resulted in a method and an instrument which offer the hematology laboratory better quality than that routinely attainable with the conventional wedge or cover-slip techniques. This spinning method provides the operator with a uniform blood \"film\" whose cell type distribution accurately reflects the distribution of the original liquid blood sample instead of a \"smear.\" This film improves the accuracy of white blood cell differential counts performed manually; it also enhances the accuracy of recently developed machines which automatically locate and classify leukocytes.", "contents": "Automatic blood film preparation by rheologically controlled spinning. During the past six years at the Biomedical Technical Center of Corning Glass Works, extensive studies on blood film preparation have resulted in a method and an instrument which offer the hematology laboratory better quality than that routinely attainable with the conventional wedge or cover-slip techniques. This spinning method provides the operator with a uniform blood \"film\" whose cell type distribution accurately reflects the distribution of the original liquid blood sample instead of a \"smear.\" This film improves the accuracy of white blood cell differential counts performed manually; it also enhances the accuracy of recently developed machines which automatically locate and classify leukocytes.", "PMID": 970408}
{"id": "pubmed23n0032_7964", "title": "Cognitive processes and academic achievement in EMR adolescents.", "content": "Twelve cognitive process variables were investigated as predictors of reading and arithmetic achievement and studied for internal structure, with 115 EMR adolescent subjects. Stepwise regression and factor analyses were employed to study prediction and structure, respectively. Memory variables were the most important predictors of reading. They were also involved in arithmetic but to a lesser extent. The data suggested that the ability to generate and utilize strategies facilitating the recall of unstructured material as well as the capacity to be sensitive to strategy-relevant structure embedded in stimuli were important prerequisites for the acquisition of reading skills. An oddity task, measuring the ability to maintain the same relational focus for successive applications to new stimulus material, was found to be most pertinent for predicting arithmetic computational skills.", "contents": "Cognitive processes and academic achievement in EMR adolescents. Twelve cognitive process variables were investigated as predictors of reading and arithmetic achievement and studied for internal structure, with 115 EMR adolescent subjects. Stepwise regression and factor analyses were employed to study prediction and structure, respectively. Memory variables were the most important predictors of reading. They were also involved in arithmetic but to a lesser extent. The data suggested that the ability to generate and utilize strategies facilitating the recall of unstructured material as well as the capacity to be sensitive to strategy-relevant structure embedded in stimuli were important prerequisites for the acquisition of reading skills. An oddity task, measuring the ability to maintain the same relational focus for successive applications to new stimulus material, was found to be most pertinent for predicting arithmetic computational skills.", "PMID": 970410}
{"id": "pubmed23n0032_7965", "title": "Dimensions of adaptive behavior of retarded and nonretarded public-school children.", "content": "The AAMD Adaptive Behavior Scale, Public School Revision, was administered to 2,618 elementary-school children from 7 to 13 years of age. The sample of children included White, Black, and Spanish-surname groups from regular and special-education classes. Factor analyses of domain scores indicated four dimensions of adaptive behavior: Functional Autonomy, Interpersonal Adjustment, Social Responsibility, and Intrapersonal Adjustment. Comparison of factor structure across school classification and age groups revealed the same four dimensions for all groups. Implications for the assessment of adaptive behavior were discussed.", "contents": "Dimensions of adaptive behavior of retarded and nonretarded public-school children. The AAMD Adaptive Behavior Scale, Public School Revision, was administered to 2,618 elementary-school children from 7 to 13 years of age. The sample of children included White, Black, and Spanish-surname groups from regular and special-education classes. Factor analyses of domain scores indicated four dimensions of adaptive behavior: Functional Autonomy, Interpersonal Adjustment, Social Responsibility, and Intrapersonal Adjustment. Comparison of factor structure across school classification and age groups revealed the same four dimensions for all groups. Implications for the assessment of adaptive behavior were discussed.", "PMID": 970411}
{"id": "pubmed23n0032_7966", "title": "Treatment of self-injurious behavior by a combination of reinforcement for incompatible behavior and overcorrection.", "content": "In two related studies, a combination of reinforcement for incompatible behavior and positive practice overcorrection was applied to the elimination of head-slapping and head-banging behavior exhibited, respectively, by two profoundly retarded boys. The design for both studies also included a period in which only reinforcement for incompatible behavior was applied. The results from Study 1 indicated that, while reinforcement had little effect in reducing the frequency of the learner's head slapping, the combination of reinforcement and overcorrection had an immediate effect in significantly reducing and eventually eliminating that behavior. A 4-month follow-up indicated no significant recurrence of the behavior. In Study 2, on the other hand, both reinforcement and the combination of reinforcement and overcorrection resulted in a significant increase in the frequency of the learner's head-banging behavior. Discussion centered on several research questions left unanswered by the differing effects of the procedures applied in both studies.", "contents": "Treatment of self-injurious behavior by a combination of reinforcement for incompatible behavior and overcorrection. In two related studies, a combination of reinforcement for incompatible behavior and positive practice overcorrection was applied to the elimination of head-slapping and head-banging behavior exhibited, respectively, by two profoundly retarded boys. The design for both studies also included a period in which only reinforcement for incompatible behavior was applied. The results from Study 1 indicated that, while reinforcement had little effect in reducing the frequency of the learner's head slapping, the combination of reinforcement and overcorrection had an immediate effect in significantly reducing and eventually eliminating that behavior. A 4-month follow-up indicated no significant recurrence of the behavior. In Study 2, on the other hand, both reinforcement and the combination of reinforcement and overcorrection resulted in a significant increase in the frequency of the learner's head-banging behavior. Discussion centered on several research questions left unanswered by the differing effects of the procedures applied in both studies.", "PMID": 970412}
{"id": "pubmed23n0032_7967", "title": "Ethnicity, geographic locale, age, sex, and urban-rural residence as variables in the prevalence of mild retardation.", "content": "The Wechsler-Intelligence Scale for Children-Revised (WISC-R) was administered to 950 of a stratified random sample of 1,040 children in Pima County, Arizona. The sample was stratified for ethnicity (Anglo, Black, Mexican-American, and Papago Indian), urban-rural residence, sex, and grade level. The three WISC-R IQ scores and cutoff points of 69 and 75 were used in comparisons of prevalence of mild mental retardation. This prevalence was significantly related to ethnicity and geographic locale. Sex, urban-rural residence, and grade level were not related to prevalence.", "contents": "Ethnicity, geographic locale, age, sex, and urban-rural residence as variables in the prevalence of mild retardation. The Wechsler-Intelligence Scale for Children-Revised (WISC-R) was administered to 950 of a stratified random sample of 1,040 children in Pima County, Arizona. The sample was stratified for ethnicity (Anglo, Black, Mexican-American, and Papago Indian), urban-rural residence, sex, and grade level. The three WISC-R IQ scores and cutoff points of 69 and 75 were used in comparisons of prevalence of mild mental retardation. This prevalence was significantly related to ethnicity and geographic locale. Sex, urban-rural residence, and grade level were not related to prevalence.", "PMID": 970413}
{"id": "pubmed23n0032_7968", "title": "Path analysis of parents' conservatism toward sex education of their mentally retarded children.", "content": "The authors investigated three probable reasons why parents of mentally retarded children would be opposed to sex education being offered as a school subject: general sexual attitudes, specific sexual attitudes, and prejudices against the retarded child's own sexuality. A questionnnaire was given to 60 parents of retarded children and 62 parents of nonretarded children. A correlation analysis revealed evidence of all three kinds of reasons and led to a causal model of conservatism on sex education. A path analysis revealed that (a) sexual prejudices are a major obstacle to sex education, and (b) each group of parents represents a specific dynamic of conservatism on sex education. Means of pedagogical intervention to modify parents' attitudes toward sex education of handicapped children were discussed.", "contents": "Path analysis of parents' conservatism toward sex education of their mentally retarded children. The authors investigated three probable reasons why parents of mentally retarded children would be opposed to sex education being offered as a school subject: general sexual attitudes, specific sexual attitudes, and prejudices against the retarded child's own sexuality. A questionnnaire was given to 60 parents of retarded children and 62 parents of nonretarded children. A correlation analysis revealed evidence of all three kinds of reasons and led to a causal model of conservatism on sex education. A path analysis revealed that (a) sexual prejudices are a major obstacle to sex education, and (b) each group of parents represents a specific dynamic of conservatism on sex education. Means of pedagogical intervention to modify parents' attitudes toward sex education of handicapped children were discussed.", "PMID": 970414}
{"id": "pubmed23n0032_7969", "title": "Comparison of nonretarded and mentally retarded children on a perceptual learning task.", "content": "The performances of 12 EMR and 12 nonretarded sixth-grade children on a perceptual learning task were compared to investigate the information processing of visually presented stimuli in individuals with different intelligence levels. A minicomputer flashed either familiar (lowercase) or unfamiliar letters (Gibson-like figures) in either a stimultaneous or successive mode on a same-different matching task. The computer automatically calculated accuracy and latency data for each subject. Results showed that accuracy was superior in the nonretarded group and that mean latencies for the EMR group was consistently higher than those of the nonretarded group. However, when latencies were corrected for differences in basic reaction time, there were no significant differences between the groups in the speed of processing the information.", "contents": "Comparison of nonretarded and mentally retarded children on a perceptual learning task. The performances of 12 EMR and 12 nonretarded sixth-grade children on a perceptual learning task were compared to investigate the information processing of visually presented stimuli in individuals with different intelligence levels. A minicomputer flashed either familiar (lowercase) or unfamiliar letters (Gibson-like figures) in either a stimultaneous or successive mode on a same-different matching task. The computer automatically calculated accuracy and latency data for each subject. Results showed that accuracy was superior in the nonretarded group and that mean latencies for the EMR group was consistently higher than those of the nonretarded group. However, when latencies were corrected for differences in basic reaction time, there were no significant differences between the groups in the speed of processing the information.", "PMID": 970415}
{"id": "pubmed23n0032_7970", "title": "Effects of price manipulations on consumer behavior in sheltered workshop token economy.", "content": "The consumer behavior of institutionalized retarded clients in a sheltered workshop token economy were evaluated by changing prices in the workshop store. In the first experiment we found that clients displayed elasticity of demand in that raising the prices of frequently purchased goods reduced the frequency and amount spent on more expendable items. Results from the second experiment showed that this change in spending pattern was not due to the relative modal unit price of item classes. The regulation of demand for consumer goods is a potentially useful way to maintain economic balance and effectiveness of a token economy.", "contents": "Effects of price manipulations on consumer behavior in sheltered workshop token economy. The consumer behavior of institutionalized retarded clients in a sheltered workshop token economy were evaluated by changing prices in the workshop store. In the first experiment we found that clients displayed elasticity of demand in that raising the prices of frequently purchased goods reduced the frequency and amount spent on more expendable items. Results from the second experiment showed that this change in spending pattern was not due to the relative modal unit price of item classes. The regulation of demand for consumer goods is a potentially useful way to maintain economic balance and effectiveness of a token economy.", "PMID": 970416}
{"id": "pubmed23n0032_7971", "title": "TMR children's competence in processing negation.", "content": "Previous researchers have suggested that TMR children lack the competence to process negation. Questions about the appropriateness of using reversible sentences to test comprehension and observations on TMR children's imitative processing of simple affirmative and negative sentences led to an experimental reexamination of the earlier findings. Institutionalized students ranging in age from 10 to 21 years with a mean IQ of 30 were asked to evaluate 16 picture pairs, 8 each for nonreversible and reversible sentences. Nonreversible sentences, both positive and negative, were interpreted correctly more often than reversible sentences. There was a significant correlation between comprehension and mental age. The results were interpreted as substantiating the adverse effect of sentence reversibility on comprehension and as evidence for the position that retarded children develop basic grammatical structures including negation at a relatively late age, but in normal interrelationship and sequence with other language and cognitive abilities.", "contents": "TMR children's competence in processing negation. Previous researchers have suggested that TMR children lack the competence to process negation. Questions about the appropriateness of using reversible sentences to test comprehension and observations on TMR children's imitative processing of simple affirmative and negative sentences led to an experimental reexamination of the earlier findings. Institutionalized students ranging in age from 10 to 21 years with a mean IQ of 30 were asked to evaluate 16 picture pairs, 8 each for nonreversible and reversible sentences. Nonreversible sentences, both positive and negative, were interpreted correctly more often than reversible sentences. There was a significant correlation between comprehension and mental age. The results were interpreted as substantiating the adverse effect of sentence reversibility on comprehension and as evidence for the position that retarded children develop basic grammatical structures including negation at a relatively late age, but in normal interrelationship and sequence with other language and cognitive abilities.", "PMID": 970417}
{"id": "pubmed23n0032_7972", "title": "Note of an error in \"Action Concept Usage by nonretarded and retarded children on structured tasks with praise for performance\".", "content": "An error in calculation of MAs of the moderately retarded subjects in the above study raises significant questions about the findings that their use of action concepts is less than that of mildly retarded subjects of the same MA. Their reduced performance may instead be attributable to some unknown extent to their lower MA.", "contents": "Note of an error in \"Action Concept Usage by nonretarded and retarded children on structured tasks with praise for performance\". An error in calculation of MAs of the moderately retarded subjects in the above study raises significant questions about the findings that their use of action concepts is less than that of mildly retarded subjects of the same MA. Their reduced performance may instead be attributable to some unknown extent to their lower MA.", "PMID": 970418}
{"id": "pubmed23n0032_7973", "title": "Dominantly inherited cystoid macular edema.", "content": "An apparently autosomal-dominant macular dystrophy occurred in three pedigrees with the presenting signs of typical cystoid macular edema due to leaking perimacular capillaries. Other striking features were retinal capillary leakage all over the posterior pole of the eye, whitish punctate deposits in the vitreous body, a normal electroretinogram, a subnormal electro-oculogram, and moderate to high hyperopia. In later or more advanced stages the macula developed a central zone of \"beaten bronze\" atrophy. Strabismus occurred frequently.", "contents": "Dominantly inherited cystoid macular edema. An apparently autosomal-dominant macular dystrophy occurred in three pedigrees with the presenting signs of typical cystoid macular edema due to leaking perimacular capillaries. Other striking features were retinal capillary leakage all over the posterior pole of the eye, whitish punctate deposits in the vitreous body, a normal electroretinogram, a subnormal electro-oculogram, and moderate to high hyperopia. In later or more advanced stages the macula developed a central zone of \"beaten bronze\" atrophy. Strabismus occurred frequently.", "PMID": 970419}
{"id": "pubmed23n0032_7974", "title": "Fluorescein angiography with an optimized filter combination.", "content": "The transmission characteristics of a new filter combination for fluorescein angiography were selected on the basis of in vivo measurements of the excitation and emission spectra of fluorescein dye during its transit in the fundus. This combination of filters was characterized by separation of 530 nm between the spectral ranges of transmission of the excitation and barrier filters. In the standard filter combinations this separation is at about 495 nm. Compared to the standard filters, the new filters enhanced the film exposure for all sites of the fundus, especially for the macular area and choroidal fluorescence. There was an improvement in demonstration of the choroidal vasculature, especially in the macular area, and better visibility of retinal detail in the macular area, but a slight decrease in contrast of the retinal vessels outside the macular region. Improved visibility of the fundus for the observer and reduction of scattering in the ocular media of older subjects improved the overall quality of angiograms made with the new filters.", "contents": "Fluorescein angiography with an optimized filter combination. The transmission characteristics of a new filter combination for fluorescein angiography were selected on the basis of in vivo measurements of the excitation and emission spectra of fluorescein dye during its transit in the fundus. This combination of filters was characterized by separation of 530 nm between the spectral ranges of transmission of the excitation and barrier filters. In the standard filter combinations this separation is at about 495 nm. Compared to the standard filters, the new filters enhanced the film exposure for all sites of the fundus, especially for the macular area and choroidal fluorescence. There was an improvement in demonstration of the choroidal vasculature, especially in the macular area, and better visibility of retinal detail in the macular area, but a slight decrease in contrast of the retinal vessels outside the macular region. Improved visibility of the fundus for the observer and reduction of scattering in the ocular media of older subjects improved the overall quality of angiograms made with the new filters.", "PMID": 970420}
{"id": "pubmed23n0032_7975", "title": "Acute pulmonary edema following intravenous fluorescein angiography.", "content": "A 64-year-old white man with no history of cardiac or bronchopulmonary disease developed acute pulmonary edema shortly after undergoing diagnostic intravenous fluorescein angiography. The patient responded quickly to a course of oxygen, positive pressure breathing, diuretics, and sedation.", "contents": "Acute pulmonary edema following intravenous fluorescein angiography. A 64-year-old white man with no history of cardiac or bronchopulmonary disease developed acute pulmonary edema shortly after undergoing diagnostic intravenous fluorescein angiography. The patient responded quickly to a course of oxygen, positive pressure breathing, diuretics, and sedation.", "PMID": 970421}
{"id": "pubmed23n0032_7976", "title": "The Miami cooperative community study on the Copeland intraocular lens (pseudophakos).", "content": "We reviewed 623 cases of Copeland iris plane implantation in an elderly population. Sixty-three percent of eyes achieved visual acuity of 6/12(20/40) or better. The incidence of significant corneal edema was low. Postoperative problems included cystoid macular edema, membranous-like structures, and iritis.", "contents": "The Miami cooperative community study on the Copeland intraocular lens (pseudophakos). We reviewed 623 cases of Copeland iris plane implantation in an elderly population. Sixty-three percent of eyes achieved visual acuity of 6/12(20/40) or better. The incidence of significant corneal edema was low. Postoperative problems included cystoid macular edema, membranous-like structures, and iritis.", "PMID": 970422}
{"id": "pubmed23n0032_7977", "title": "Intraocular penetrating of amoxicillin.", "content": "The enucleated eyes of New Zealand albino rabbits were injected with 50, 100, 250, and 500 mg/kg of amoxicillin to determine the drug penetration into the aqueous humor, vitreous humor, serum, and other ocular tissues after oral administration. Detectable levels were achieved after each specific dose for at least five hours. In the aqueous humor, drug levels were present at 15 minutes, peaked at 1.5 and two hours, and were present at leave five hours after oral administration.", "contents": "Intraocular penetrating of amoxicillin. The enucleated eyes of New Zealand albino rabbits were injected with 50, 100, 250, and 500 mg/kg of amoxicillin to determine the drug penetration into the aqueous humor, vitreous humor, serum, and other ocular tissues after oral administration. Detectable levels were achieved after each specific dose for at least five hours. In the aqueous humor, drug levels were present at 15 minutes, peaked at 1.5 and two hours, and were present at leave five hours after oral administration.", "PMID": 970423}
{"id": "pubmed23n0032_7978", "title": "Experimental intravitreal gentamicin.", "content": "Rabbits received intravitreal injections of gentamicin and indirect ophthalmoscopy, electroretinography, and histopathology determined relative toxicity. An intravitreal dose of 0.1 to 0.2 mg of commercially available gentamicin was well tolerated in the rabbit eye. A dosage of 0.4 mg or higher consistently produced significant ocular toxicity as evidenced by extinction of the electroretinogram, gross fundus changes, and histopathologic alterations.", "contents": "Experimental intravitreal gentamicin. Rabbits received intravitreal injections of gentamicin and indirect ophthalmoscopy, electroretinography, and histopathology determined relative toxicity. An intravitreal dose of 0.1 to 0.2 mg of commercially available gentamicin was well tolerated in the rabbit eye. A dosage of 0.4 mg or higher consistently produced significant ocular toxicity as evidenced by extinction of the electroretinogram, gross fundus changes, and histopathologic alterations.", "PMID": 970424}
{"id": "pubmed23n0032_7979", "title": "Levodopa-stimulated growth hormone secretion and diabetic retinopathy.", "content": "Since growth hormone has been implicated as a possible etiologic factor in the development of diabetic retinopathy, we examined growth hormone levels in two groups of growth-onset diabetics matched for age and duration of disease. The experimental group had definite proliferative diabetic retinopathy; the control group of growth-onset diabetics had no significant retinopathy. Basal and levodopa-stimulated levels of growth hormone were determined for each group. Growth hormone response could not be correlated with the presence or absence of diabetic retinopathy (P less than .05).", "contents": "Levodopa-stimulated growth hormone secretion and diabetic retinopathy. Since growth hormone has been implicated as a possible etiologic factor in the development of diabetic retinopathy, we examined growth hormone levels in two groups of growth-onset diabetics matched for age and duration of disease. The experimental group had definite proliferative diabetic retinopathy; the control group of growth-onset diabetics had no significant retinopathy. Basal and levodopa-stimulated levels of growth hormone were determined for each group. Growth hormone response could not be correlated with the presence or absence of diabetic retinopathy (P less than .05).", "PMID": 970425}
{"id": "pubmed23n0032_7980", "title": "Spontaneous hyphema associated with ingestion of aspirin and ethanol.", "content": "Unilateral hyphema, hematuria, and ecchymoses developed in a previously healthy 42-year-old women after the ingestion of aspirin and ethanol. There was no evidence for ocular trauma, disease, or vascular malformation by slit-lamp examination and gonioscopy. Platelet count and coagulation tests were normal. The patient's bleeding time was prolonged and there was impaired platelet aggregation. Delayed (secondary) aggregation in response to collagen, adenosine diphosphate, and epinephrine was decreased, as was aggregation induced by thrombin and serotonin. These data indicate that the qualitative platelet defect was induced by both aspirin and ethanol. Anterior chamber hemorrhage subsided after discontinuation of aspirin and ethanol, and the hyphema subsequently resolved. Bleeding time and platelet aggregation were normal two weeks after the patient's initial presentation. A prolonged bleeding time in association with normal platelet count, prothrombin time, and partial thromboplastin time indicated a qualitative platelet defect, which is most commonly drug-induced. Defective platelet function resulted in spontaneous hyphema.", "contents": "Spontaneous hyphema associated with ingestion of aspirin and ethanol. Unilateral hyphema, hematuria, and ecchymoses developed in a previously healthy 42-year-old women after the ingestion of aspirin and ethanol. There was no evidence for ocular trauma, disease, or vascular malformation by slit-lamp examination and gonioscopy. Platelet count and coagulation tests were normal. The patient's bleeding time was prolonged and there was impaired platelet aggregation. Delayed (secondary) aggregation in response to collagen, adenosine diphosphate, and epinephrine was decreased, as was aggregation induced by thrombin and serotonin. These data indicate that the qualitative platelet defect was induced by both aspirin and ethanol. Anterior chamber hemorrhage subsided after discontinuation of aspirin and ethanol, and the hyphema subsequently resolved. Bleeding time and platelet aggregation were normal two weeks after the patient's initial presentation. A prolonged bleeding time in association with normal platelet count, prothrombin time, and partial thromboplastin time indicated a qualitative platelet defect, which is most commonly drug-induced. Defective platelet function resulted in spontaneous hyphema.", "PMID": 970426}
{"id": "pubmed23n0032_7981", "title": "Spontaneous cyclic segmental sphincter spasms in an Adie's tonic pupil.", "content": "A 43-year-old woman with bilateral Adie's tonic pupils had a segment of one iris sphincter that contracted spontaneously every few seconds, but was not influenced by light, near vision, or eye movements.", "contents": "Spontaneous cyclic segmental sphincter spasms in an Adie's tonic pupil. A 43-year-old woman with bilateral Adie's tonic pupils had a segment of one iris sphincter that contracted spontaneously every few seconds, but was not influenced by light, near vision, or eye movements.", "PMID": 970428}
{"id": "pubmed23n0032_7982", "title": "Management of the thumb-index web space contracture.", "content": "This paper focuses on the management of the thumb-index web space contracture, a contracture that can interfere significantly with hand function. The anatomy of the thumb-index web space and the contracture mechanism are reviewed. Contractures frequently result from traumatic hand injuries involving the web space or peripheral nerves. Exercises and splinting techniques for preventing or correcting these contractures are directed at achieving and maintaining maximum range of motion and strength of the thumb. Case reviews are reported that show improvements in range of motion measurements of 31 patients treated with the described techniques.", "contents": "Management of the thumb-index web space contracture. This paper focuses on the management of the thumb-index web space contracture, a contracture that can interfere significantly with hand function. The anatomy of the thumb-index web space and the contracture mechanism are reviewed. Contractures frequently result from traumatic hand injuries involving the web space or peripheral nerves. Exercises and splinting techniques for preventing or correcting these contractures are directed at achieving and maintaining maximum range of motion and strength of the thumb. Case reviews are reported that show improvements in range of motion measurements of 31 patients treated with the described techniques.", "PMID": 970429}
{"id": "pubmed23n0032_7983", "title": "3 theoretical approaches to play: applications for exceptional children.", "content": "Three theories of play are reviewed and discussed in terms of their applicability to exceptional children. The theories--Freud's psychodynamic, Piaget's developmental, and Ellis' arousal-seeking--are compared on the basis of: 1. the validity of their constructs, 2. their ability to account for behaviors of exceptional children, and 3. the likelihood that they could generate future play research with an exceptional population. It was concluded that the only theory that could hope to meet these requirements at this time was the arousal-seeking theory of Ellis.", "contents": "3 theoretical approaches to play: applications for exceptional children. Three theories of play are reviewed and discussed in terms of their applicability to exceptional children. The theories--Freud's psychodynamic, Piaget's developmental, and Ellis' arousal-seeking--are compared on the basis of: 1. the validity of their constructs, 2. their ability to account for behaviors of exceptional children, and 3. the likelihood that they could generate future play research with an exceptional population. It was concluded that the only theory that could hope to meet these requirements at this time was the arousal-seeking theory of Ellis.", "PMID": 970430}
{"id": "pubmed23n0032_7984", "title": "Components in program development.", "content": "There has been a great deal of discussion and deliberation concerning the composition of program development. Nonetheless, the question still remains--what is program development? What are possible components in program development? In this paper the following components are described, differentiated, and integrated: Statement of Purpose, Curriculum Design, Program Competencies, Content, Content Competencies, and Practice Environments.", "contents": "Components in program development. There has been a great deal of discussion and deliberation concerning the composition of program development. Nonetheless, the question still remains--what is program development? What are possible components in program development? In this paper the following components are described, differentiated, and integrated: Statement of Purpose, Curriculum Design, Program Competencies, Content, Content Competencies, and Practice Environments.", "PMID": 970431}
{"id": "pubmed23n0032_7985", "title": "The role of environmental ammonia in respiratory mycoplasmosis of rats.", "content": "Young adult, pathogen-free rats of Sherman and Fischer (F344) substrains were inoculated intranasally with 10(8) colony-forming units (GFU) of M. pulmonis and housed for 4 to 6 weeks in environments with ammonia maintained at specific concentrations from 25 to 250 ppm. All levels of NH3--whether produced naturally from soiled bedding or derived from a purified source--significantly increased the severity of the rhinitis, otitis media, tracheitis, and pneumonia (including bronchiectasis) characteristic of murine respiratory mycoplasmosis (MRM). The prevalence of pneumonia, but not that of other respiratory lesions of MRM, showed a strong tendency to increase directly with environmental NH3 concentration. In contrast, NH3 exposure of rats not infected with M. pulmonis caused anatomic lesions that were unlike those of MRM and were limited to the nasal passages. It was concluded that environmental NH3, at concentrations commonly encountered in present day cage environments for rats, plays an important role in pathogenesis of MRM.", "contents": "The role of environmental ammonia in respiratory mycoplasmosis of rats. Young adult, pathogen-free rats of Sherman and Fischer (F344) substrains were inoculated intranasally with 10(8) colony-forming units (GFU) of M. pulmonis and housed for 4 to 6 weeks in environments with ammonia maintained at specific concentrations from 25 to 250 ppm. All levels of NH3--whether produced naturally from soiled bedding or derived from a purified source--significantly increased the severity of the rhinitis, otitis media, tracheitis, and pneumonia (including bronchiectasis) characteristic of murine respiratory mycoplasmosis (MRM). The prevalence of pneumonia, but not that of other respiratory lesions of MRM, showed a strong tendency to increase directly with environmental NH3 concentration. In contrast, NH3 exposure of rats not infected with M. pulmonis caused anatomic lesions that were unlike those of MRM and were limited to the nasal passages. It was concluded that environmental NH3, at concentrations commonly encountered in present day cage environments for rats, plays an important role in pathogenesis of MRM.", "PMID": 970435}
{"id": "pubmed23n0032_7986", "title": "The pathology of viral hepatitis types A and B in chimpanzees. A comparison.", "content": "The histologic manifestations in the livers of chimpanzees inoculated with hepatitis A and B virus were compared with each other and correlated with biochemical, serologic, and virologic observations. Both types of hepatitis reveal alterations similar to those seen in human hepatitis, but the lesions--particularly the hepatocellular necrosis--are far milder. Hepatitis Type A in chimpanzees is a disease of short incubation period and duration. The hepatocytic alterations are mainly restricted to the periportal areas, and the parenchymal changes are less severe than the portal inflammation. The lesions correlated well with biochemical changes, the presence of virus in the liver, and its shedding in the stool. In contrast, experimental Type B hepatitis has a long incubation period and longer duration, involves the entire lobular parenchyma, and is, if anything, more severe in the lobular centers while portal inflammation is less conspicuous. Biochemical alterations and presence of virus in the liver correlate with these lesions, and the antibody response is similar to that seen in man. The chimpanzee is a useful model for studying the pathogenesis of viral hepatitis; additional study of serial morphologic events may contribute to our understanding of the clinical differences between hepatitis Type A and Type B.", "contents": "The pathology of viral hepatitis types A and B in chimpanzees. A comparison. The histologic manifestations in the livers of chimpanzees inoculated with hepatitis A and B virus were compared with each other and correlated with biochemical, serologic, and virologic observations. Both types of hepatitis reveal alterations similar to those seen in human hepatitis, but the lesions--particularly the hepatocellular necrosis--are far milder. Hepatitis Type A in chimpanzees is a disease of short incubation period and duration. The hepatocytic alterations are mainly restricted to the periportal areas, and the parenchymal changes are less severe than the portal inflammation. The lesions correlated well with biochemical changes, the presence of virus in the liver, and its shedding in the stool. In contrast, experimental Type B hepatitis has a long incubation period and longer duration, involves the entire lobular parenchyma, and is, if anything, more severe in the lobular centers while portal inflammation is less conspicuous. Biochemical alterations and presence of virus in the liver correlate with these lesions, and the antibody response is similar to that seen in man. The chimpanzee is a useful model for studying the pathogenesis of viral hepatitis; additional study of serial morphologic events may contribute to our understanding of the clinical differences between hepatitis Type A and Type B.", "PMID": 970436}
{"id": "pubmed23n0032_7987", "title": "Isolation of myotoxic component from rattlesnake (Crotalus viridis viridis) venom. Electron microscopic analysis of muscle damage.", "content": "The pathogenesis of myonecrosis induced by a purified component of rattlesnake (Crotalus viridis viridis) venom was studied at the light and electron microscopic levels. Crude venom was fractionated by gel filtration (Sephadex G-50) followed by cation exchange chromatography (Sephadex C-25). Electrophoretic homogeneity of the isolated myotoxin (Fraction II from C-25 column) was demonstrated in isoelectric focusing and disc gel polyacrylamide gel electrophoresis. White mice were injected intramuscularly with 1.5 mug/g of the purified protein in 0.1 ml of physiologic saline. Light microscopic examination of injected muscle revealed a series of degenerative events including partial vacuolation of muscle cells at 6, 12, and 24 hours and complete vacuolation and loss of striations at 48 and 72 hours. Hemorrhage was not observed. At the electron microscopic level the perinuclear space and sarcoplasmic reticulum were dilated in all samples. By 48 and 72 hours the myofibrils lacked striations and the sarcomeres were disorganized. Plasma membranes and T tubules remained intact in all samples. These results correlated well with the myonecrosis induced by crude Crotalus viridis viridis venom except for several important aspects. The pure component altered skeletal muscle cells specifically, with the sarcoplasmic reticulum being the primary site of action.", "contents": "Isolation of myotoxic component from rattlesnake (Crotalus viridis viridis) venom. Electron microscopic analysis of muscle damage. The pathogenesis of myonecrosis induced by a purified component of rattlesnake (Crotalus viridis viridis) venom was studied at the light and electron microscopic levels. Crude venom was fractionated by gel filtration (Sephadex G-50) followed by cation exchange chromatography (Sephadex C-25). Electrophoretic homogeneity of the isolated myotoxin (Fraction II from C-25 column) was demonstrated in isoelectric focusing and disc gel polyacrylamide gel electrophoresis. White mice were injected intramuscularly with 1.5 mug/g of the purified protein in 0.1 ml of physiologic saline. Light microscopic examination of injected muscle revealed a series of degenerative events including partial vacuolation of muscle cells at 6, 12, and 24 hours and complete vacuolation and loss of striations at 48 and 72 hours. Hemorrhage was not observed. At the electron microscopic level the perinuclear space and sarcoplasmic reticulum were dilated in all samples. By 48 and 72 hours the myofibrils lacked striations and the sarcomeres were disorganized. Plasma membranes and T tubules remained intact in all samples. These results correlated well with the myonecrosis induced by crude Crotalus viridis viridis venom except for several important aspects. The pure component altered skeletal muscle cells specifically, with the sarcoplasmic reticulum being the primary site of action.", "PMID": 970437}
{"id": "pubmed23n0032_7988", "title": "Ultrastructural observations on experimentally produced melanin pigmentation of the corneal epithelium.", "content": "Melanin pigmentation of the corneal epithelium was induced in pigmented guinea pigs by the topical application of colchicine to their eyes or by corneal cauterization with silver nitrate. With colchicine the pigmentation was preceded by the development of an abnormal corneal epithelium in which numerous cells became arrested in cell division. The corneal melanosis resulted largely from the migration of melanocytes into the corneal epithelium from the normally pigmented contiguous conjunctiva and to a lesser extent from the presence of melanin granules within corneal epithelial cells. In both models a leukocytic and vascular invasion of the cornea proceded and accompanied the migration of melanocytes into the corneal epithelium. Electron microscopy disclosed cells with the same morphology as conjunctival melanocytes between the epithelial cells of the cornea. Mature melanin granules were also present within some squamous epithelial cells as individual granules or as clusters. The ultrastructural findings are viewed in relation to how melanin granules are transferred from melanocytes to epithelial cells. Evidence is presented which suggests that malanin granule transfer may follow the fusion of the membranes of the melanocytes and epithelial cells.", "contents": "Ultrastructural observations on experimentally produced melanin pigmentation of the corneal epithelium. Melanin pigmentation of the corneal epithelium was induced in pigmented guinea pigs by the topical application of colchicine to their eyes or by corneal cauterization with silver nitrate. With colchicine the pigmentation was preceded by the development of an abnormal corneal epithelium in which numerous cells became arrested in cell division. The corneal melanosis resulted largely from the migration of melanocytes into the corneal epithelium from the normally pigmented contiguous conjunctiva and to a lesser extent from the presence of melanin granules within corneal epithelial cells. In both models a leukocytic and vascular invasion of the cornea proceded and accompanied the migration of melanocytes into the corneal epithelium. Electron microscopy disclosed cells with the same morphology as conjunctival melanocytes between the epithelial cells of the cornea. Mature melanin granules were also present within some squamous epithelial cells as individual granules or as clusters. The ultrastructural findings are viewed in relation to how melanin granules are transferred from melanocytes to epithelial cells. Evidence is presented which suggests that malanin granule transfer may follow the fusion of the membranes of the melanocytes and epithelial cells.", "PMID": 970438}
{"id": "pubmed23n0032_7989", "title": "Goiter formation following prostaglandin administration in rats.", "content": "Prostaglandins (PGE1 and PGE2) induced a hyperplastic microfollicular goiter with a high radioiodine (131I) thyroid uptake, increased endocytosis, a heavy autoradiographic (125I) reaction, and a moderate increase of thyroid hormones (T4, T3), thyroxine-binding globulin (TGB), and thyrotropin (TSH) concentrations in adult rats. Ultrastructurally, both prostaglandins (E1 and E2) markedly stimulated the thyroid cell activity and increased the number of pseudopodia, the size of colloid and dense granule populations, and the number of polysomes. Conversely, a hypofunction of thyroid glands with low radioiodine (131I) thyroid uptake, a decreased autoradiographic (125I) reaction, and a moderate decrease in T4, T3, TGB, and TSH concentrations were observed following prostaglandin F 2alpha. Ultrastructurally, a decrease in size of the colloid and dense granule population and the number of degenerative mitochondria occurred infollicular cells. An intense hyperplasia of parafollicular (C) cells, with abundant population of characteristic dense granules, could be seen in PGF 2alpha-treated rats. A marked decrease of radioiodine (131I) uptake, endocytosis, and autoradiographic (125I) reaction and a sharp decline in T4, T3, and TBG were observed in hypophysectomized and chronically prostaglandin-treated rats. Light and electron microscopy revealed signs of an advanced thyroid hypofunction with flat cuboidal cells, reduced microvilli, scarce endoplasmic reticulum, and few dense droplets. The present findings demonstrate that the chronic administration of prostaglandins exerts significant effects of thyroid gland and goiter formation (goitrogenesis), radioiodine metabolism, and hormone synthesis, and that these effects are mediated by TSH secretion.", "contents": "Goiter formation following prostaglandin administration in rats. Prostaglandins (PGE1 and PGE2) induced a hyperplastic microfollicular goiter with a high radioiodine (131I) thyroid uptake, increased endocytosis, a heavy autoradiographic (125I) reaction, and a moderate increase of thyroid hormones (T4, T3), thyroxine-binding globulin (TGB), and thyrotropin (TSH) concentrations in adult rats. Ultrastructurally, both prostaglandins (E1 and E2) markedly stimulated the thyroid cell activity and increased the number of pseudopodia, the size of colloid and dense granule populations, and the number of polysomes. Conversely, a hypofunction of thyroid glands with low radioiodine (131I) thyroid uptake, a decreased autoradiographic (125I) reaction, and a moderate decrease in T4, T3, TGB, and TSH concentrations were observed following prostaglandin F 2alpha. Ultrastructurally, a decrease in size of the colloid and dense granule population and the number of degenerative mitochondria occurred infollicular cells. An intense hyperplasia of parafollicular (C) cells, with abundant population of characteristic dense granules, could be seen in PGF 2alpha-treated rats. A marked decrease of radioiodine (131I) uptake, endocytosis, and autoradiographic (125I) reaction and a sharp decline in T4, T3, and TBG were observed in hypophysectomized and chronically prostaglandin-treated rats. Light and electron microscopy revealed signs of an advanced thyroid hypofunction with flat cuboidal cells, reduced microvilli, scarce endoplasmic reticulum, and few dense droplets. The present findings demonstrate that the chronic administration of prostaglandins exerts significant effects of thyroid gland and goiter formation (goitrogenesis), radioiodine metabolism, and hormone synthesis, and that these effects are mediated by TSH secretion.", "PMID": 970439}
{"id": "pubmed23n0032_7990", "title": "Reticular cell hyperplasia and amyloidosis in a line of mice with low leukocyte counts.", "content": "Two pathologic conditions, reticular cell hyperplasia and amyloidosis, were studied in a strain of mice selectively bred for low leukocyte counts (LLC). At the age of 3 to 6 months, 70% of the mice developed reticular cell hyperplasia in the inguinea lymph nodes, and at 11 to 18 months, about 100% of them developed amyloidosis in the spleen and in the kidney, liver, and adrenal glands. Immunofluorescence was revealed in the glomeruli, interstitium of the tubules, and the amyloid skeleton of the papilla when the kidney sections were incubated with fluorescein-labeled antimouse immunoglobulins. Both intracellular and extracellular amyloid fibrils were found in the liver, spleen, and kidney sections by electron microscopy. Distended rough endoplasmic reticulum (RER) cisternae and hypertrophy of RER with or without the simultaneous presence of amyloid fibrils in the reticular cells of the spleen and Kupffer cells of the liver were observed. In this light, we believe that the pathologic conditions are manifestations of immunologic events that are characteristic of the LLC mice with immune deficiency or abnormality in the immune system. We conclude that the origin of amyloid protein is at the RER. We discuss processes of amyloid fibril formation and some genetic aspects of amyloid development.", "contents": "Reticular cell hyperplasia and amyloidosis in a line of mice with low leukocyte counts. Two pathologic conditions, reticular cell hyperplasia and amyloidosis, were studied in a strain of mice selectively bred for low leukocyte counts (LLC). At the age of 3 to 6 months, 70% of the mice developed reticular cell hyperplasia in the inguinea lymph nodes, and at 11 to 18 months, about 100% of them developed amyloidosis in the spleen and in the kidney, liver, and adrenal glands. Immunofluorescence was revealed in the glomeruli, interstitium of the tubules, and the amyloid skeleton of the papilla when the kidney sections were incubated with fluorescein-labeled antimouse immunoglobulins. Both intracellular and extracellular amyloid fibrils were found in the liver, spleen, and kidney sections by electron microscopy. Distended rough endoplasmic reticulum (RER) cisternae and hypertrophy of RER with or without the simultaneous presence of amyloid fibrils in the reticular cells of the spleen and Kupffer cells of the liver were observed. In this light, we believe that the pathologic conditions are manifestations of immunologic events that are characteristic of the LLC mice with immune deficiency or abnormality in the immune system. We conclude that the origin of amyloid protein is at the RER. We discuss processes of amyloid fibril formation and some genetic aspects of amyloid development.", "PMID": 970442}
{"id": "pubmed23n0032_7991", "title": "Histopathology of spontaneous regression in virus-induced murine leukemia.", "content": "The histopathology of the spontaneous regression of murine leukemia induced by a particular strain of Friend leukemia virus was studied in Swiss ICR/Ha mice. Animals inoculated with the regressing strain of Friend virus exhibited an initial pathologic response identical to that induced by conventional strains of Friend virus. Unlike the fatal leukemia produced by conventional Friend virus, the pathology of the disease induced by the regressing strain of Friend virus appeared to be self-limiting. The histopathology of the two diseases is compared in this report.", "contents": "Histopathology of spontaneous regression in virus-induced murine leukemia. The histopathology of the spontaneous regression of murine leukemia induced by a particular strain of Friend leukemia virus was studied in Swiss ICR/Ha mice. Animals inoculated with the regressing strain of Friend virus exhibited an initial pathologic response identical to that induced by conventional strains of Friend virus. Unlike the fatal leukemia produced by conventional Friend virus, the pathology of the disease induced by the regressing strain of Friend virus appeared to be self-limiting. The histopathology of the two diseases is compared in this report.", "PMID": 970443}
{"id": "pubmed23n0032_7992", "title": "Striated membranous structures in human hearts. An ultrastructural study.", "content": "Straited membranous structures (SMS), which consisted of sheets or ribbons of 130 to 220 A in thickness, showed variable patterns of periodic substructure, and resembled SMS described in renal and ocular tissues in various diseases, were found in extracellular locations in a) mitral valve (2 patients) and tricuspid valve (1 patient) of 2 patients with mitral valvular prolapse, b) mitral valve and femoral artery of 1 patient with Marfan's syndrome and prolapsed mitral valve, and c) myocardium (2 patients) and thickened endocardium (3 patients) of 3 patients with congenital heart disease associated with muscular obstruction to right ventricular outflow. Striated membranous structures measured up to several microns in diameter, often were highly folded and convoluted, and sometimes appeared circular in outline. Some SMS measured from 130 to 150 A in thickness and had indistinct edges and poorly defined periodicity. The majority of SMS, however, had greater thicknesses, in the range of 200 A, and a periodicity characterized by alternating light and dark bands with a spacing that varied from 100 to 160 A. The structures were associated with thickened basement membranes, elastic fibers, and membrane-bound bodies of the type thought to be involved in elastogenesis. Evidence available suggests that SMS results from an unusual pattern of arrangement of a component, possibly Type IV collagen, of basement membrane material.", "contents": "Striated membranous structures in human hearts. An ultrastructural study. Straited membranous structures (SMS), which consisted of sheets or ribbons of 130 to 220 A in thickness, showed variable patterns of periodic substructure, and resembled SMS described in renal and ocular tissues in various diseases, were found in extracellular locations in a) mitral valve (2 patients) and tricuspid valve (1 patient) of 2 patients with mitral valvular prolapse, b) mitral valve and femoral artery of 1 patient with Marfan's syndrome and prolapsed mitral valve, and c) myocardium (2 patients) and thickened endocardium (3 patients) of 3 patients with congenital heart disease associated with muscular obstruction to right ventricular outflow. Striated membranous structures measured up to several microns in diameter, often were highly folded and convoluted, and sometimes appeared circular in outline. Some SMS measured from 130 to 150 A in thickness and had indistinct edges and poorly defined periodicity. The majority of SMS, however, had greater thicknesses, in the range of 200 A, and a periodicity characterized by alternating light and dark bands with a spacing that varied from 100 to 160 A. The structures were associated with thickened basement membranes, elastic fibers, and membrane-bound bodies of the type thought to be involved in elastogenesis. Evidence available suggests that SMS results from an unusual pattern of arrangement of a component, possibly Type IV collagen, of basement membrane material.", "PMID": 970444}
{"id": "pubmed23n0032_7993", "title": "The morphologic similarities of human and canine globoid leukodystrophy. Thin section and freeze-fracture studies.", "content": "Human and canine globoid leukodystrophy are shown to be indistinguishable morphologically. Both have characteristic deposits with polygonal cross-sectional profiles in addition to twisted ribbon-like structures. The specificity of the deposits is emphasized, and their laminated nature is corroborated by the freeze-fracture studies. Their apparently hollow profiles in cross section are thought to be produced by the tight arrangement of the bilayers that prevent penetration of the stain.", "contents": "The morphologic similarities of human and canine globoid leukodystrophy. Thin section and freeze-fracture studies. Human and canine globoid leukodystrophy are shown to be indistinguishable morphologically. Both have characteristic deposits with polygonal cross-sectional profiles in addition to twisted ribbon-like structures. The specificity of the deposits is emphasized, and their laminated nature is corroborated by the freeze-fracture studies. Their apparently hollow profiles in cross section are thought to be produced by the tight arrangement of the bilayers that prevent penetration of the stain.", "PMID": 970445}
{"id": "pubmed23n0032_7994", "title": "Effect of dehydration on stimulation of ADH release by heterologous renin infusions in conscious dogs.", "content": "Unanesthetized dogs were infused with heterologous (hog) renin at 0.33 Goldblatt U/kg per h for 2 h, once normally hydrated and once after 48 h of dehydration. Dehydration increased the average plasma osmolality from 306 to 322 mosmol/kg, the plasma renin activity (PRA) from 0.5 to 1.4 ng/ml per h, and the plasma antidiuretic hormone (ADH) concentration from 1.7 to 3.7 muU/ml, although the latter was not statistically significant. Renin infusion resulted in approximately the same average PRA, about 10 ng/ml per h, in both states of hydration. Mean arterial blood pressure increased during renin infusion in both states of hydration, although the increase was greater when the dogs were normally hydrated. There was no apparent effect of renin infusion on plasma ADH concentration when the dogs were normally hydrated, but in the dehydrated state renin infusion was accompanied by an increase from 3.7 to 6.3 muU/ml in plasma ADH concentrations after 80 min of infusion. There were no apparent changes in plasma osmolality or sodium or potassium concentrations due to the renin infusions; however, plasma osmolality and potassium concentration decreased during the course of the experiment. The results suggest a possible role for the renin-angiotensin system of renin released by the kidney in the control of ADH during dehydration. The metabolic clearance rate of the hog renin was 37 ml/min-kg.", "contents": "Effect of dehydration on stimulation of ADH release by heterologous renin infusions in conscious dogs. Unanesthetized dogs were infused with heterologous (hog) renin at 0.33 Goldblatt U/kg per h for 2 h, once normally hydrated and once after 48 h of dehydration. Dehydration increased the average plasma osmolality from 306 to 322 mosmol/kg, the plasma renin activity (PRA) from 0.5 to 1.4 ng/ml per h, and the plasma antidiuretic hormone (ADH) concentration from 1.7 to 3.7 muU/ml, although the latter was not statistically significant. Renin infusion resulted in approximately the same average PRA, about 10 ng/ml per h, in both states of hydration. Mean arterial blood pressure increased during renin infusion in both states of hydration, although the increase was greater when the dogs were normally hydrated. There was no apparent effect of renin infusion on plasma ADH concentration when the dogs were normally hydrated, but in the dehydrated state renin infusion was accompanied by an increase from 3.7 to 6.3 muU/ml in plasma ADH concentrations after 80 min of infusion. There were no apparent changes in plasma osmolality or sodium or potassium concentrations due to the renin infusions; however, plasma osmolality and potassium concentration decreased during the course of the experiment. The results suggest a possible role for the renin-angiotensin system of renin released by the kidney in the control of ADH during dehydration. The metabolic clearance rate of the hog renin was 37 ml/min-kg.", "PMID": 970447}
{"id": "pubmed23n0032_7995", "title": "Canine gut receptors mediating pancreatic responses to luminal L-amino acids.", "content": "Pancreatic protein and bicarbonate outputs were measured in dogs with pancreatic fistulas receiving intravenous secretin while individual L-amino acids or mixtures of L-amino acids were perfused into proximal intestine, Both phenylalanine and tryptophan were potent stimuli of pancreatic secretion; alanine, leucine, and valine increased protein outputs but their effects were small and not statistically significant. Six other amino acids were ineffective. Tryptophan perfused together with phenylalanine augmented responses to phenylalanine; methionine, ineffective when perfused alone, did not alter responses to phenylalanine when perfused with the latter. Responses to phenylalanine perfused at concentrations above 8 mM were dependent on load and were mediated by lengths of proximal bowel greater than 10 cm.", "contents": "Canine gut receptors mediating pancreatic responses to luminal L-amino acids. Pancreatic protein and bicarbonate outputs were measured in dogs with pancreatic fistulas receiving intravenous secretin while individual L-amino acids or mixtures of L-amino acids were perfused into proximal intestine, Both phenylalanine and tryptophan were potent stimuli of pancreatic secretion; alanine, leucine, and valine increased protein outputs but their effects were small and not statistically significant. Six other amino acids were ineffective. Tryptophan perfused together with phenylalanine augmented responses to phenylalanine; methionine, ineffective when perfused alone, did not alter responses to phenylalanine when perfused with the latter. Responses to phenylalanine perfused at concentrations above 8 mM were dependent on load and were mediated by lengths of proximal bowel greater than 10 cm.", "PMID": 970448}
{"id": "pubmed23n0032_7996", "title": "Canine pancreatic response to intestinally perfused oligopeptides.", "content": "Although older work indicated that luminal peptides are stimulants of pancreatic secretion, these earlier experiments were performed with crude peptide mixtures containing amino acids that are also known stimulants. Furthermore, no information was provided about size or composition of stimulating peptides. For this reason, the problem was reinvestigated with commercially synthesized oligopeptides in dogs equipped with chronic gastric and pancreatic fistulas. Synthetic peptides at 30 mM concentrations were perfused into the proximal bowel when luminal pancreatic proteases were reduced to undetectable concentrations and dogs were receiving intravenous exogenous secretin infusions. Increases in pancreatic outputs of protein and bicarbonate were measured. Of the peptides tested, only glycylphenylalanine, glycyltryptophan, and phenylalanylglycine stimulated, whereas both di- and triglycine were without effect. It was concluded that some, but not all, oligopeptides in the gut lumen are stimulants of pancreatic secretion.", "contents": "Canine pancreatic response to intestinally perfused oligopeptides. Although older work indicated that luminal peptides are stimulants of pancreatic secretion, these earlier experiments were performed with crude peptide mixtures containing amino acids that are also known stimulants. Furthermore, no information was provided about size or composition of stimulating peptides. For this reason, the problem was reinvestigated with commercially synthesized oligopeptides in dogs equipped with chronic gastric and pancreatic fistulas. Synthetic peptides at 30 mM concentrations were perfused into the proximal bowel when luminal pancreatic proteases were reduced to undetectable concentrations and dogs were receiving intravenous exogenous secretin infusions. Increases in pancreatic outputs of protein and bicarbonate were measured. Of the peptides tested, only glycylphenylalanine, glycyltryptophan, and phenylalanylglycine stimulated, whereas both di- and triglycine were without effect. It was concluded that some, but not all, oligopeptides in the gut lumen are stimulants of pancreatic secretion.", "PMID": 970449}
{"id": "pubmed23n0032_7997", "title": "Canine pancreatic responses to intestinally perfused proteins and protein digests.", "content": "Pancreatic bicarbonate and protein secretory responses to intestinally perfused proteins or digests of proteins were measured in dogs with chronic gastric and pancreatic fistulas when luminalpancreatic protease concentrations were reduced to undetectable levels. Protein digests were analyzed for amino acid content, and various other indirect met-ods were used to assess the composition of the digest mixtures. Of five undigested proteins, none evoked more pancreatic secretion than a control perfusion with saline. Peptic digestion of these same proteins converted four of them to polypeptides that were poten stimuli of a pancreatic juice similar in HCO3-/protein ratios to that evoked by luminal amino acids. Dialyzed peptic digests of one of the proteins, bovine serum albumin (BSA), retained potency. Likewise, digestion of BSA with endogenous or exogenous pancreatic proteases converted the protein to a stimulus about equipotent with the peptic digest, though the composition of the pancreatic digests differed markedly from that of the peptic digests. We conclude that a) luminal peptides of four or more amino acids can stimulate the pancreas and b) during protein alimentation a wide array of luminal protein products may evoke pancreatic secretion.", "contents": "Canine pancreatic responses to intestinally perfused proteins and protein digests. Pancreatic bicarbonate and protein secretory responses to intestinally perfused proteins or digests of proteins were measured in dogs with chronic gastric and pancreatic fistulas when luminalpancreatic protease concentrations were reduced to undetectable levels. Protein digests were analyzed for amino acid content, and various other indirect met-ods were used to assess the composition of the digest mixtures. Of five undigested proteins, none evoked more pancreatic secretion than a control perfusion with saline. Peptic digestion of these same proteins converted four of them to polypeptides that were poten stimuli of a pancreatic juice similar in HCO3-/protein ratios to that evoked by luminal amino acids. Dialyzed peptic digests of one of the proteins, bovine serum albumin (BSA), retained potency. Likewise, digestion of BSA with endogenous or exogenous pancreatic proteases converted the protein to a stimulus about equipotent with the peptic digest, though the composition of the pancreatic digests differed markedly from that of the peptic digests. We conclude that a) luminal peptides of four or more amino acids can stimulate the pancreas and b) during protein alimentation a wide array of luminal protein products may evoke pancreatic secretion.", "PMID": 970450}
{"id": "pubmed23n0032_7998", "title": "Adrenergic receptors mediating depolarization in brown adipose tissue.", "content": "Adrenergic receptors mediating depolarization in in vitro neonatal rat brown adipose tissue (BAT) have been characterized by use of adrenergic agonists and antagonists. Releasable endogenous catecholamine was present in BAT as demonstrated by tyramine- and 1,1-dimethyl-4-phenylpiperazinium iodide- (DMPP) induced depolarization in BAT from normal rats and its absence when BAT from reserpinized rats was used. In BAT from reserpinized rats l-norepinephrine, l-phenylephrine, and l-isoproterenol all similarly depolarized the bronw adipocytes over the concentration range of 10(-8) to 10(-6) M with a maximal depolarization of about 25 mV. Dopamine and d-norepinephrine were more than 100 times less potent. The beta-adrenergic blocker propranolol competitively inhibited isoproterenol-induced depolarization, whereas the alpha-adrenergic blackers, phentolamine and phenoxybenzamine, inhibited the phenylephrine-induced depolarization with much smaller inhibitory effects on the isoproterenol-induced depolarization. Both phenylephrine and isoproterenol elicited transient depolarizations when briefly added to the bathing medium while continuously recording from the same cell. Both the agonist and antagonist studies are interpreted as indicating the presence of both alpha- and beta-adrenergic receptors on BAT cells which mediate catecholamine-induced depolarization.", "contents": "Adrenergic receptors mediating depolarization in brown adipose tissue. Adrenergic receptors mediating depolarization in in vitro neonatal rat brown adipose tissue (BAT) have been characterized by use of adrenergic agonists and antagonists. Releasable endogenous catecholamine was present in BAT as demonstrated by tyramine- and 1,1-dimethyl-4-phenylpiperazinium iodide- (DMPP) induced depolarization in BAT from normal rats and its absence when BAT from reserpinized rats was used. In BAT from reserpinized rats l-norepinephrine, l-phenylephrine, and l-isoproterenol all similarly depolarized the bronw adipocytes over the concentration range of 10(-8) to 10(-6) M with a maximal depolarization of about 25 mV. Dopamine and d-norepinephrine were more than 100 times less potent. The beta-adrenergic blocker propranolol competitively inhibited isoproterenol-induced depolarization, whereas the alpha-adrenergic blackers, phentolamine and phenoxybenzamine, inhibited the phenylephrine-induced depolarization with much smaller inhibitory effects on the isoproterenol-induced depolarization. Both phenylephrine and isoproterenol elicited transient depolarizations when briefly added to the bathing medium while continuously recording from the same cell. Both the agonist and antagonist studies are interpreted as indicating the presence of both alpha- and beta-adrenergic receptors on BAT cells which mediate catecholamine-induced depolarization.", "PMID": 970451}
{"id": "pubmed23n0032_7999", "title": "Mechanisms of temperature regulation in heat-acclimated hamsters.", "content": "Mechanisms of temperature regulation were assessed by measurements of oxygen consumption (VO2), body temperature (Rre = rectal, Tsk = skin), evaporative water loss (EWL), regional distribution of blood flow, and blood volume. Hamsters (Mesocricetus auratus) were acclimated to ambient temperatures of 34 or 22 degrees C. VO2 of 34 degrees C-exposed animals was reduced to 50% of that of controls at 22 degrees C, whereas EWL with heat exposure was almost double that of controls. Heat-acclimated animals had a slightly elevated Tre in comparison to 22 degrees C-acclimated animals, whereas there was a marked elevation in Tsk with heat exposure, in contrast to control animals at 22 degrees C. Blood flow distribution measurements indicated that with 34 degrees C exposure there was a decreased flow in liver, kidney, and intestine, whereas there was an increase to the carcass. Red cell and plasma volumes in heat-acclimated hamsters were decreased belwo the values of the 22 degrees C controls. Heat acclimation of the fhamster appears to involve reduced VO2 and increased WEL. Convective and radiative heat loss appear to be maintained by increased Tsk with heat exposure. Nonevaporative heat dissipation mechanisms are of primary importance in thermoregulation of the heat-acclimated hamster, and it is suggested that this is mediated by increased peripheral blood flow with reduced flow to the viscera.", "contents": "Mechanisms of temperature regulation in heat-acclimated hamsters. Mechanisms of temperature regulation were assessed by measurements of oxygen consumption (VO2), body temperature (Rre = rectal, Tsk = skin), evaporative water loss (EWL), regional distribution of blood flow, and blood volume. Hamsters (Mesocricetus auratus) were acclimated to ambient temperatures of 34 or 22 degrees C. VO2 of 34 degrees C-exposed animals was reduced to 50% of that of controls at 22 degrees C, whereas EWL with heat exposure was almost double that of controls. Heat-acclimated animals had a slightly elevated Tre in comparison to 22 degrees C-acclimated animals, whereas there was a marked elevation in Tsk with heat exposure, in contrast to control animals at 22 degrees C. Blood flow distribution measurements indicated that with 34 degrees C exposure there was a decreased flow in liver, kidney, and intestine, whereas there was an increase to the carcass. Red cell and plasma volumes in heat-acclimated hamsters were decreased belwo the values of the 22 degrees C controls. Heat acclimation of the fhamster appears to involve reduced VO2 and increased WEL. Convective and radiative heat loss appear to be maintained by increased Tsk with heat exposure. Nonevaporative heat dissipation mechanisms are of primary importance in thermoregulation of the heat-acclimated hamster, and it is suggested that this is mediated by increased peripheral blood flow with reduced flow to the viscera.", "PMID": 970452}
{"id": "pubmed23n0032_8000", "title": "Inhibition of proximal tubular hydrolysis and reabsorption of bradykinin by peptides.", "content": "[3H] bradykinin ([3H] BKN) was microinfused alone or in the presence of a 390- or 780-fold excess of BKN or angiotensin I (AI) into proximal tubules in Inactin-anesthetized rats. Urinary excretion of 3H-labeled material was measured, and intact peptide and its metabolites were identified and quantified. When [3H] BKN was administered with BKN or AI, urinary recovery of 3H-labeled material was increased in a manner directly proportional to tubular length, suggesting that reabsorption of [3H] BKN is related to extent of tubular contact. BKN and AI were equally effective in inhibiting the reabosroption of [3H] BKN and its metabolites from proximal tubular fluid. In contrast, BKN but not AI effectively inhibited the enzymatic hydrolysis of [3H] BKN in the proximal tubule, The data suggest that the proximal tubular mechanism for reabsorbing BKN and its metabolites is of high capacity but not high specificity and that the mechanisms for enzymatic cleavage and reabsorption of BKN and its metabolites may had different specificites and capacities.", "contents": "Inhibition of proximal tubular hydrolysis and reabsorption of bradykinin by peptides. [3H] bradykinin ([3H] BKN) was microinfused alone or in the presence of a 390- or 780-fold excess of BKN or angiotensin I (AI) into proximal tubules in Inactin-anesthetized rats. Urinary excretion of 3H-labeled material was measured, and intact peptide and its metabolites were identified and quantified. When [3H] BKN was administered with BKN or AI, urinary recovery of 3H-labeled material was increased in a manner directly proportional to tubular length, suggesting that reabsorption of [3H] BKN is related to extent of tubular contact. BKN and AI were equally effective in inhibiting the reabosroption of [3H] BKN and its metabolites from proximal tubular fluid. In contrast, BKN but not AI effectively inhibited the enzymatic hydrolysis of [3H] BKN in the proximal tubule, The data suggest that the proximal tubular mechanism for reabsorbing BKN and its metabolites is of high capacity but not high specificity and that the mechanisms for enzymatic cleavage and reabsorption of BKN and its metabolites may had different specificites and capacities.", "PMID": 970453}
{"id": "pubmed23n0032_8001", "title": "Glucose and bicarbonate reabsorption in edematous dogs.", "content": "Glucose and bicarbonate reabsorption were studied in dogs made edematour by aortocaval fistula (A-V dogs) and in sham-operated dogs. Following construction of the A-V fistula, there was a significant increase in body weight; glomerular filtration rate, renal plasma flow, hematocrit, and sodium excretion decreased significantly. Bicarbonate reabosorption was significantly higher in A-V than in sham dogs both during control and volume expansion. Volume expansion depressed bicarbonate reabsorption significantly in both groups. Glucose reabsorption fell following volume expansion in both groups; glucose reabsorption was significantly higher in A-V dogs than in sham dogs during control and volume expansion. Volume expansion led to a minimal increase in sodium excretion in A-V dogs when compared to the increase in the sham dogs. These data demonsttate that chronic sodium retention is associated with enhanced reabsorption of glucose and bicarbonate. The site in the neophron responsible for the increase in reabsorption of these substances cannot be determined with certainty based on these clearance studies although it is possible that proximal reabsorption may be enhanced in this model.", "contents": "Glucose and bicarbonate reabsorption in edematous dogs. Glucose and bicarbonate reabsorption were studied in dogs made edematour by aortocaval fistula (A-V dogs) and in sham-operated dogs. Following construction of the A-V fistula, there was a significant increase in body weight; glomerular filtration rate, renal plasma flow, hematocrit, and sodium excretion decreased significantly. Bicarbonate reabosorption was significantly higher in A-V than in sham dogs both during control and volume expansion. Volume expansion depressed bicarbonate reabsorption significantly in both groups. Glucose reabsorption fell following volume expansion in both groups; glucose reabsorption was significantly higher in A-V dogs than in sham dogs during control and volume expansion. Volume expansion led to a minimal increase in sodium excretion in A-V dogs when compared to the increase in the sham dogs. These data demonsttate that chronic sodium retention is associated with enhanced reabsorption of glucose and bicarbonate. The site in the neophron responsible for the increase in reabsorption of these substances cannot be determined with certainty based on these clearance studies although it is possible that proximal reabsorption may be enhanced in this model.", "PMID": 970454}
{"id": "pubmed23n0032_8002", "title": "Effect of prostaglandin E2 on ovine maternal placental blood flow.", "content": "The effect of PGE2 on regional blood flows in the chronically catheterized near-term pregnant sheep was investigated using radioactive microspheres. The injection of 20 mug PGE2 per kilogram into the left ventricle of eight sheep resulted in no change in maternal brain and noncotyledonary uterine flow. The renal blood flow increased from 692 to 892 ml/min (P less than 0.004). The uterine blood flow decreased from 673 to 317 ml/min (P less than 0.001). The trium was bypassed by injecting 7 mug PGE2 per kilogram of sheep into a fetal venous catheter and permitting it to reach the placental vasculature after placental transfer. Eleven sets of observations were made in eight animals. We observed no change in the intrauterine pressure, maternal brain flow, and noncotyledonary uterine blood flow secondary to this procedure. The maternal renal blood flow changed from 592 to 669 ml/min (P less than 0.007). The uterine blood flow increased from 762 to 853 ml/min (P less than 0.02). The uterine vascular resistance decreased from 0.124 to 0.115 mmHg x min/ml (P less than 0.04). It was concluded that 1) PGE3 crosses the placenta quite readily, and 2) PGE3 causes dilatation of the maternal placental vascular bed.", "contents": "Effect of prostaglandin E2 on ovine maternal placental blood flow. The effect of PGE2 on regional blood flows in the chronically catheterized near-term pregnant sheep was investigated using radioactive microspheres. The injection of 20 mug PGE2 per kilogram into the left ventricle of eight sheep resulted in no change in maternal brain and noncotyledonary uterine flow. The renal blood flow increased from 692 to 892 ml/min (P less than 0.004). The uterine blood flow decreased from 673 to 317 ml/min (P less than 0.001). The trium was bypassed by injecting 7 mug PGE2 per kilogram of sheep into a fetal venous catheter and permitting it to reach the placental vasculature after placental transfer. Eleven sets of observations were made in eight animals. We observed no change in the intrauterine pressure, maternal brain flow, and noncotyledonary uterine blood flow secondary to this procedure. The maternal renal blood flow changed from 592 to 669 ml/min (P less than 0.007). The uterine blood flow increased from 762 to 853 ml/min (P less than 0.02). The uterine vascular resistance decreased from 0.124 to 0.115 mmHg x min/ml (P less than 0.04). It was concluded that 1) PGE3 crosses the placenta quite readily, and 2) PGE3 causes dilatation of the maternal placental vascular bed.", "PMID": 970455}
{"id": "pubmed23n0032_8003", "title": "D-Glucose enhancement of water reabsorption in proximal tubule of the rat kidney.", "content": "Water reabsorption in the proximal convoluted tubule of the rat kidney was examined by in vivo microperfusion techniques in order to examine the effect of D-glucose within the tubular lumen. When tubules were perfused with a balanced artificial solution containing Na, K, Cl, HCO3, urea, and D-glucose, absolute reabsorption averaged 4.01 +/- 0.24 nl/min per mm. Addition of D-glucose to the NaCl perfusate enhanced water reabsorption to values similar to those obtained with the balanced artificial perfusate. The enhanced water reabsorption consequent to the addition of D-glucose to the NaCl perfusion solution was completely inhibited by addition of phloridzin to the perfusate. The addition of an unabsorbed hexose, 2-deoxy-D-glucose, to the NaCl perfusate failed to enhance water reabsorption, whereas the addition of an incompletely reabsorbed sugar that is not metabolized, 3-O-methyl-D-glucose, resulted in partial enhancement of theabsolute rate of water reabsorption. These studies demonstrate that D-glucose has the specific effect of augmenting water reabsorption in the proximal tubule of the rat kidney.", "contents": "D-Glucose enhancement of water reabsorption in proximal tubule of the rat kidney. Water reabsorption in the proximal convoluted tubule of the rat kidney was examined by in vivo microperfusion techniques in order to examine the effect of D-glucose within the tubular lumen. When tubules were perfused with a balanced artificial solution containing Na, K, Cl, HCO3, urea, and D-glucose, absolute reabsorption averaged 4.01 +/- 0.24 nl/min per mm. Addition of D-glucose to the NaCl perfusate enhanced water reabsorption to values similar to those obtained with the balanced artificial perfusate. The enhanced water reabsorption consequent to the addition of D-glucose to the NaCl perfusion solution was completely inhibited by addition of phloridzin to the perfusate. The addition of an unabsorbed hexose, 2-deoxy-D-glucose, to the NaCl perfusate failed to enhance water reabsorption, whereas the addition of an incompletely reabsorbed sugar that is not metabolized, 3-O-methyl-D-glucose, resulted in partial enhancement of theabsolute rate of water reabsorption. These studies demonstrate that D-glucose has the specific effect of augmenting water reabsorption in the proximal tubule of the rat kidney.", "PMID": 970456}
{"id": "pubmed23n0032_8004", "title": "Cat heart acetylcholine: structural proof and distribution.", "content": "The distribution of acetylcholine (ACh) in the cat heart was investigated by a pyrolysis-gas chromatography (PGC) method. The hearts were dissected into various regions and homogenized in acetonitrile in the presence of propionylcholine, internal standard. Following extraction with toluene and hexane, the choline esters were precipitated as the enneaiodide complex. The isolated choline esters were analyzed by PGC, and the peak corresponding to ACh was quantified. The compound extracted from heart tissue that eluted with the retention time of authentic ACh was identified by mass spectrometry as dimethylaminoethylacetate, the pyrolysis product of ACh. ACh concentrations were found to be higher in the atria than the ventricles. In both the atria and the ventricles, a higher content of ACh was found in the right than the left portions: right ventricle, 5.0 compared to left ventricle, 2.0 nmol/g; and right atrium, 16.8 compared to left atrium, 11.3 nmol/g. Some cats were subjected to a bilateral cervical vagotomy 3 wk before removal and analysis of heart tissue. Hearts from vagotomized cats contained less ACh than controls in the right ventricle (-31%), right atrium (-54%), SA node (-42%), and papillary muscle (-53%), but no decreases were found in the left ventricle, left atrium, or interventricular septum.", "contents": "Cat heart acetylcholine: structural proof and distribution. The distribution of acetylcholine (ACh) in the cat heart was investigated by a pyrolysis-gas chromatography (PGC) method. The hearts were dissected into various regions and homogenized in acetonitrile in the presence of propionylcholine, internal standard. Following extraction with toluene and hexane, the choline esters were precipitated as the enneaiodide complex. The isolated choline esters were analyzed by PGC, and the peak corresponding to ACh was quantified. The compound extracted from heart tissue that eluted with the retention time of authentic ACh was identified by mass spectrometry as dimethylaminoethylacetate, the pyrolysis product of ACh. ACh concentrations were found to be higher in the atria than the ventricles. In both the atria and the ventricles, a higher content of ACh was found in the right than the left portions: right ventricle, 5.0 compared to left ventricle, 2.0 nmol/g; and right atrium, 16.8 compared to left atrium, 11.3 nmol/g. Some cats were subjected to a bilateral cervical vagotomy 3 wk before removal and analysis of heart tissue. Hearts from vagotomized cats contained less ACh than controls in the right ventricle (-31%), right atrium (-54%), SA node (-42%), and papillary muscle (-53%), but no decreases were found in the left ventricle, left atrium, or interventricular septum.", "PMID": 970457}
{"id": "pubmed23n0032_8005", "title": "Ascending pressor and depressor pathways in the cat spinal cord.", "content": "Blood pressure responses to stimulation of the cervical dorsolateral sulcus (DLS) of the spinal cord and lumbar dorsal roots were studied in anesthetized, vagotomized, and paralyzed cats. Stimulation of the lumbar dorsal roots elicited pressor responses with high-frequency stimulation (50 Hz, 10 V, 1 ms) and depressor responses with low-grequency stimulation (1 Hz, 10 V, 1 ms). Pressor responses were converted to depressor responses after bilateral lesions were made in the DLS area rostral to the site of stimulation. These results suggest that the ascending spinal pressor pathways are localized in the DLS region. Furthermore, these depressor responses were abolished by placing additional bilateral lesions in the dorsolateral funiculus (DLF) rostral to the site of stimulation. These data indicate the presence of ascending depressor pathways in the DLF which are anatomically separate from pressor pathways. Both pathways were found to be bilateral systems, and decussation of fibers appears to be complete within three segments rostral to their entry into the spinal cord. Ascending depressor pathways have a low optimal frequency of activation, as opposed to the ascending pressor pathways which have a relatively high optimal frequency of activation. Neurophysiological evidence obtained by recording unit activity from the cervical sympathetic trunk confirmed the localization of the ascending pressor and depressor pathways.", "contents": "Ascending pressor and depressor pathways in the cat spinal cord. Blood pressure responses to stimulation of the cervical dorsolateral sulcus (DLS) of the spinal cord and lumbar dorsal roots were studied in anesthetized, vagotomized, and paralyzed cats. Stimulation of the lumbar dorsal roots elicited pressor responses with high-frequency stimulation (50 Hz, 10 V, 1 ms) and depressor responses with low-grequency stimulation (1 Hz, 10 V, 1 ms). Pressor responses were converted to depressor responses after bilateral lesions were made in the DLS area rostral to the site of stimulation. These results suggest that the ascending spinal pressor pathways are localized in the DLS region. Furthermore, these depressor responses were abolished by placing additional bilateral lesions in the dorsolateral funiculus (DLF) rostral to the site of stimulation. These data indicate the presence of ascending depressor pathways in the DLF which are anatomically separate from pressor pathways. Both pathways were found to be bilateral systems, and decussation of fibers appears to be complete within three segments rostral to their entry into the spinal cord. Ascending depressor pathways have a low optimal frequency of activation, as opposed to the ascending pressor pathways which have a relatively high optimal frequency of activation. Neurophysiological evidence obtained by recording unit activity from the cervical sympathetic trunk confirmed the localization of the ascending pressor and depressor pathways.", "PMID": 970458}
{"id": "pubmed23n0032_8006", "title": "Effect of histamine on intestinal fluid secretion in the dog.", "content": "Intra-arterial infusion of histamine into the small intestine caused about a onefold increase of blood flow, edema of the intestinal tissues and mesentery, and produced a copious secretion of fluid. The jejunal secretions had an ionic composition similar to that of plasma, whereas ileal secretions contained high concentrations of HCO3 with relative low concentrations of Cl. The secretions contained protein (1.5 +/- .2 g/100 ml, range 0.5-2.4) with a similar electrophoretic pattern of plasma protein. When lissamine green was present in the blood, it also appeared in the secretion to a considerable concentration. It is inferred from these findings that a major mechanism of fluid secretion by the action of histamine involves a filtration process across the mucosal epithelium by the incrreased tissue fluid pressure due to extensive capillary leak.", "contents": "Effect of histamine on intestinal fluid secretion in the dog. Intra-arterial infusion of histamine into the small intestine caused about a onefold increase of blood flow, edema of the intestinal tissues and mesentery, and produced a copious secretion of fluid. The jejunal secretions had an ionic composition similar to that of plasma, whereas ileal secretions contained high concentrations of HCO3 with relative low concentrations of Cl. The secretions contained protein (1.5 +/- .2 g/100 ml, range 0.5-2.4) with a similar electrophoretic pattern of plasma protein. When lissamine green was present in the blood, it also appeared in the secretion to a considerable concentration. It is inferred from these findings that a major mechanism of fluid secretion by the action of histamine involves a filtration process across the mucosal epithelium by the incrreased tissue fluid pressure due to extensive capillary leak.", "PMID": 970459}
{"id": "pubmed23n0032_8007", "title": "Changes in CNS responsiveness during hibernation.", "content": "The ability of the midbrain reticular formation (MRF) to produce thermogenic responses and to trigger arousal from hibernation was tested during successive quarters of individual hibernation bouts. Golden-mantled ground squirrels (Citellus lateralis) were implanted with bilateral cannula guides into the MRF. Single, bilateral, 1-mul injections of acetylcholine (ACh) at concentrations of 50, 100, or 200 mug/mul were delivered in each quarter of the same bout or in selected quarters of different bouts. The results show that the magnitude of thermogenic responses evoked by ACh stimulation of the MRF was depressed during the early portion of the bout and increased as time in the bout elapsed. Furthermore, the magnitude of responses evoked during hibernation was depressed in comparison to those evoked during euthermia, indicating the influence of inhibition on the responsiveness of the CNS during hibernation. We suggest that during hibernation, a progressive change in responsiveness of the CNS, perhpas focused in the MRF, controls the duration of each hibernation bout.", "contents": "Changes in CNS responsiveness during hibernation. The ability of the midbrain reticular formation (MRF) to produce thermogenic responses and to trigger arousal from hibernation was tested during successive quarters of individual hibernation bouts. Golden-mantled ground squirrels (Citellus lateralis) were implanted with bilateral cannula guides into the MRF. Single, bilateral, 1-mul injections of acetylcholine (ACh) at concentrations of 50, 100, or 200 mug/mul were delivered in each quarter of the same bout or in selected quarters of different bouts. The results show that the magnitude of thermogenic responses evoked by ACh stimulation of the MRF was depressed during the early portion of the bout and increased as time in the bout elapsed. Furthermore, the magnitude of responses evoked during hibernation was depressed in comparison to those evoked during euthermia, indicating the influence of inhibition on the responsiveness of the CNS during hibernation. We suggest that during hibernation, a progressive change in responsiveness of the CNS, perhpas focused in the MRF, controls the duration of each hibernation bout.", "PMID": 970460}
{"id": "pubmed23n0032_8008", "title": "Correlation of plasma corticosterone levels with running activity in the blinded rat.", "content": "Blinded female reats were placed in running-wheel cages to monitor the phase of their activity cycle. After approximately a month of adaptation to the wheels, jugular vein blood samples were withdrawn at the beginning and end of the running phase of activity and analyzed for plasma corticosterone. By the time blood samples were obtained, the blined rats' activity cycles had desynchronized from the lighting cycle of the room in an apparently free-running fashion, and were out of phase with each other. The corticosterone levels were high at the beginning of the running phase and low at the end: begin run corticosterone was 34.5 +/- 13, end run corticosteronewas 14.2 +/- 10 (mean +/-SD in mug/100 ml of plasma; t = 7.93, df = 82, P less than 0.001). It was concluded that blinded rats do have an adrenocortical rhythm and that it is in phase with the activity cycle of each individual rat.", "contents": "Correlation of plasma corticosterone levels with running activity in the blinded rat. Blinded female reats were placed in running-wheel cages to monitor the phase of their activity cycle. After approximately a month of adaptation to the wheels, jugular vein blood samples were withdrawn at the beginning and end of the running phase of activity and analyzed for plasma corticosterone. By the time blood samples were obtained, the blined rats' activity cycles had desynchronized from the lighting cycle of the room in an apparently free-running fashion, and were out of phase with each other. The corticosterone levels were high at the beginning of the running phase and low at the end: begin run corticosterone was 34.5 +/- 13, end run corticosteronewas 14.2 +/- 10 (mean +/-SD in mug/100 ml of plasma; t = 7.93, df = 82, P less than 0.001). It was concluded that blinded rats do have an adrenocortical rhythm and that it is in phase with the activity cycle of each individual rat.", "PMID": 970461}
{"id": "pubmed23n0032_8009", "title": "Early postglomerular plasma concentrations of chloride, sodium, and inulin in the rat kidney.", "content": "Sodium, chloride, and inulin concentrations were measured in plasma collected from the terminal portions of long efferent vessels at the subcapsular surface of the rat kidney. Sodium concentration equaled and the concentrations of chloride and inulin were less than those in peripheral plasma. During benzolamide infusion, chloride concentration equaled while inulin concentration remained less than in peripheral plasma. In free-flow micropuncture samples collected randomly during control conditions, chloride concentration rose rapidly in the early proximal tubule and then remained elevated and constant throughout the remainder of the proximal tubule accessible to micropuncture. These experiments indicate that normally tubular reabsorbate low in chloride and inulin is added to the blood traversing the early postglomerular vessels before reaching the kidney surface. Bases on the analyses of proximal tubular fluid, this type of reabsorbate appears available only from the early proximal tubular segment. We conclude that a close functional relationship exists between the first segment of the proximal tubule and the early postglomerular blood supply characteristic of the superficial cortical nephron.", "contents": "Early postglomerular plasma concentrations of chloride, sodium, and inulin in the rat kidney. Sodium, chloride, and inulin concentrations were measured in plasma collected from the terminal portions of long efferent vessels at the subcapsular surface of the rat kidney. Sodium concentration equaled and the concentrations of chloride and inulin were less than those in peripheral plasma. During benzolamide infusion, chloride concentration equaled while inulin concentration remained less than in peripheral plasma. In free-flow micropuncture samples collected randomly during control conditions, chloride concentration rose rapidly in the early proximal tubule and then remained elevated and constant throughout the remainder of the proximal tubule accessible to micropuncture. These experiments indicate that normally tubular reabsorbate low in chloride and inulin is added to the blood traversing the early postglomerular vessels before reaching the kidney surface. Bases on the analyses of proximal tubular fluid, this type of reabsorbate appears available only from the early proximal tubular segment. We conclude that a close functional relationship exists between the first segment of the proximal tubule and the early postglomerular blood supply characteristic of the superficial cortical nephron.", "PMID": 970462}
{"id": "pubmed23n0032_8010", "title": "Development of the renal response to blood volume expansion in normal and fast-growing rats.", "content": "Male rats from reduced (fast-growing) litters between 14 and 50 days of age were studied. Standard renal clearnce techniques were employed. After a 60-min control period, the animals were infused (2.3% body wt) with heparinized donor blood obtained from lillermates of the same age. Renal function was followed for an additional 60 min. The efficiency of the diuretic response, the percent infused volume excreted above control levels, and the sodium efficiency, the percent infused Na excreted, were calculated. Results indicate that both efficiencies develop in a discontinuous pattern and that they are comparable. This pattern of development, as well as the magnitude of the mature response, is comparable to that previously reported for rats from intact (normally growing) litters. The onset and attainment of the mature response is, however, shifted in time, such that reduced-litter animals achieve the mature response 10-15 days earlier than intact-litter rats. Results exclude chronological age or body weight alone as principal determinant of the mature response and suggest that some function of growth rate is responsible for the maturation of this regulatory function.", "contents": "Development of the renal response to blood volume expansion in normal and fast-growing rats. Male rats from reduced (fast-growing) litters between 14 and 50 days of age were studied. Standard renal clearnce techniques were employed. After a 60-min control period, the animals were infused (2.3% body wt) with heparinized donor blood obtained from lillermates of the same age. Renal function was followed for an additional 60 min. The efficiency of the diuretic response, the percent infused volume excreted above control levels, and the sodium efficiency, the percent infused Na excreted, were calculated. Results indicate that both efficiencies develop in a discontinuous pattern and that they are comparable. This pattern of development, as well as the magnitude of the mature response, is comparable to that previously reported for rats from intact (normally growing) litters. The onset and attainment of the mature response is, however, shifted in time, such that reduced-litter animals achieve the mature response 10-15 days earlier than intact-litter rats. Results exclude chronological age or body weight alone as principal determinant of the mature response and suggest that some function of growth rate is responsible for the maturation of this regulatory function.", "PMID": 970463}
{"id": "pubmed23n0032_8011", "title": "Effect of rapid temperature change on mean body temperature and gill ventilation in carp.", "content": "Carp were transferred from 25 degrees C water to a calorimeter at 15 degrees C or from 15 degrees C water to a calorimeter at 25 degrees C. During cooling, dorsal muscle temperature (Tdm) and mean body temperature (Tb) were continuously monitored. During the first minutes Tb changed rapidly, while Tdm changed only slightly. In a 0.468-dg fish, Tdm decreased to two-thirds of the final value in 8.05 min, while Tb attained this value in 3.6 min. Tdm changed faster during heating than during cooling. Carp were also placed in a device where gill ventilation (VG) could be continuously monitored while rapid shifts in the temperature of the inspired water (TPINSP) AND/OR BODY SURFACE (Tbs) were effected. Changing Tinsp and Tbs both effected rate-sensitive changes in VG, although Tinsp was more effective. Decreases in Tinsp or Tbs produced decreases in VG; increases in Tinsp or Tbs had an opposite effect. The fish respiratory system is sensitive to the rate of change of external temperature. This sensitivity provides evidence for the existence of peripheral thermal input to the systems regulating respiration in teleost fish.", "contents": "Effect of rapid temperature change on mean body temperature and gill ventilation in carp. Carp were transferred from 25 degrees C water to a calorimeter at 15 degrees C or from 15 degrees C water to a calorimeter at 25 degrees C. During cooling, dorsal muscle temperature (Tdm) and mean body temperature (Tb) were continuously monitored. During the first minutes Tb changed rapidly, while Tdm changed only slightly. In a 0.468-dg fish, Tdm decreased to two-thirds of the final value in 8.05 min, while Tb attained this value in 3.6 min. Tdm changed faster during heating than during cooling. Carp were also placed in a device where gill ventilation (VG) could be continuously monitored while rapid shifts in the temperature of the inspired water (TPINSP) AND/OR BODY SURFACE (Tbs) were effected. Changing Tinsp and Tbs both effected rate-sensitive changes in VG, although Tinsp was more effective. Decreases in Tinsp or Tbs produced decreases in VG; increases in Tinsp or Tbs had an opposite effect. The fish respiratory system is sensitive to the rate of change of external temperature. This sensitivity provides evidence for the existence of peripheral thermal input to the systems regulating respiration in teleost fish.", "PMID": 970464}
{"id": "pubmed23n0032_8012", "title": "Sex and estrogens in protection against circulatory stress reactions.", "content": "From Wistar rats, data are presented which indicate that inbred females a) have a greater ability to clear particulate matter from the blood stream than do males of the same strain; b) are significantly more resistant to two different forms of lethal circulatory stress (e.g., intestinal ischemia and whole-body trauma) than are males; and c) exhibit a greater resistance to undergo reticuloendothelial system (RES) phagocytic depression after both forms of lethal trauma than do males. Estradiol treatment of males, using either acute, massive (1 or 10 mg/kg) or multiple, low dose (10 or 100 mug/kg) regimens, confers trauma resistance on such animals. Such estradiol treated male rats exhibit hyperactive RES's. These estradiol-treated males, when subjected to either lethal ischemia or trauma, fail to demonstrate the early RES phagocytic depression seen in untreated controls. Untreated female as well as estradiol-treated male rats exhibit significantly higher arterial blood pressures post-trauma than do untreated male rats. Direct microscopic observation of rat mesenteries indicr whole-b0dy trauma, the untreated females as well as the estradiol-treated males exhibit significantly less dilatation of microscopic capacitance vessels (i.e., venules) than do untreated male rats. The data reported herein could be used to suggest that estrogenic hormones may play pivotal roles in a) the amelioration of an organism's reaction to systemic stress; and b) control of macrophage and peripheral vascular functions.", "contents": "Sex and estrogens in protection against circulatory stress reactions. From Wistar rats, data are presented which indicate that inbred females a) have a greater ability to clear particulate matter from the blood stream than do males of the same strain; b) are significantly more resistant to two different forms of lethal circulatory stress (e.g., intestinal ischemia and whole-body trauma) than are males; and c) exhibit a greater resistance to undergo reticuloendothelial system (RES) phagocytic depression after both forms of lethal trauma than do males. Estradiol treatment of males, using either acute, massive (1 or 10 mg/kg) or multiple, low dose (10 or 100 mug/kg) regimens, confers trauma resistance on such animals. Such estradiol treated male rats exhibit hyperactive RES's. These estradiol-treated males, when subjected to either lethal ischemia or trauma, fail to demonstrate the early RES phagocytic depression seen in untreated controls. Untreated female as well as estradiol-treated male rats exhibit significantly higher arterial blood pressures post-trauma than do untreated male rats. Direct microscopic observation of rat mesenteries indicr whole-b0dy trauma, the untreated females as well as the estradiol-treated males exhibit significantly less dilatation of microscopic capacitance vessels (i.e., venules) than do untreated male rats. The data reported herein could be used to suggest that estrogenic hormones may play pivotal roles in a) the amelioration of an organism's reaction to systemic stress; and b) control of macrophage and peripheral vascular functions.", "PMID": 970465}
{"id": "pubmed23n0032_8013", "title": "Osmoltye and tryptophan receptors controlling gastric emptying in the dog.", "content": "The effect of three monosaccharides, three disaccharides, two dipeptides, combinations of tryptophan with two hexoses, one hexitol, and two amino acids ongastric emptying was studied in dogs to further define the samll intestinal receptors responsive to osmolytes and tryptophan. On a molar basis the disacchardies and dipeptides were almost twice as potent as their respective constituent monosaccharides or amino acids implying that the osmoreceptor is deep to the brush border disaccharidases and cytosol dipeptidases. Tryptophan probably acts by a mechanism different from the osmoreceptor since slowing of gastric emptying by tryptophan was inhibited by methionine which has no effect on a stimulant of the osmoreceptor mechanism. Lysine unlike methionine does not share the neutral amino acid transport pathway with tryptophan. Lysine did not change the inhibitory effect of tryptophan on gastric emptying. This imples that transport of tryptophan into the intestinal cell is necessary for its slowing effect. Glucose and galactose also inhibited the tryptophan effect whereas a nonabsorbed hexitor, mannitol, was without effect. Interference by the hexoses was also probably by competition with tryptophan for transport into the cell. These studies further indicate that the tryptophan receptor is different from the osmoreceptor.", "contents": "Osmoltye and tryptophan receptors controlling gastric emptying in the dog. The effect of three monosaccharides, three disaccharides, two dipeptides, combinations of tryptophan with two hexoses, one hexitol, and two amino acids ongastric emptying was studied in dogs to further define the samll intestinal receptors responsive to osmolytes and tryptophan. On a molar basis the disacchardies and dipeptides were almost twice as potent as their respective constituent monosaccharides or amino acids implying that the osmoreceptor is deep to the brush border disaccharidases and cytosol dipeptidases. Tryptophan probably acts by a mechanism different from the osmoreceptor since slowing of gastric emptying by tryptophan was inhibited by methionine which has no effect on a stimulant of the osmoreceptor mechanism. Lysine unlike methionine does not share the neutral amino acid transport pathway with tryptophan. Lysine did not change the inhibitory effect of tryptophan on gastric emptying. This imples that transport of tryptophan into the intestinal cell is necessary for its slowing effect. Glucose and galactose also inhibited the tryptophan effect whereas a nonabsorbed hexitor, mannitol, was without effect. Interference by the hexoses was also probably by competition with tryptophan for transport into the cell. These studies further indicate that the tryptophan receptor is different from the osmoreceptor.", "PMID": 970466}
{"id": "pubmed23n0032_8014", "title": "Pathways regulating cardiovascular changes during volume loading in awake dogs.", "content": "The role played by the cardiac sympathetic nerves and arterial baroreceptors in the cardiovascular responses to acute volume loading was studied in conscious dogs. In 15 normally innervated animals, mean arterial pressure rose 10 mmHg, heart rate increased 38 beats/min and cardiac output 1,696 ml/min, while peripheral resistance decreased 0.99 PRU. Neither bilateral baroreceptor denervation, dorsal root sections (T1-T5) or surgical interruption of the left ansa subclavia altered the above responses to acute volume loading. Bilateral section of the ansa subclavia (total cardiac sympathectomy) significantly reduced the heart rate response from 35 +/- 5 to 20 +/- 5 beats/min but did not alter other changes. A similar reduction in heart rate response was observed following selective section of the right ansa subclavia. Intravenous infusions of epinephrine augmented the heart rate response in both normally innervated and cardiac sympathectomized dogs. It is suggested that although the primary efferent pathway for the reflex trachycardia is via the vagus, responses are modulated by sympathetic neural activity. Additionally, the nervous system was not shown to play a measureable role in the observed peripheral resistance changes.", "contents": "Pathways regulating cardiovascular changes during volume loading in awake dogs. The role played by the cardiac sympathetic nerves and arterial baroreceptors in the cardiovascular responses to acute volume loading was studied in conscious dogs. In 15 normally innervated animals, mean arterial pressure rose 10 mmHg, heart rate increased 38 beats/min and cardiac output 1,696 ml/min, while peripheral resistance decreased 0.99 PRU. Neither bilateral baroreceptor denervation, dorsal root sections (T1-T5) or surgical interruption of the left ansa subclavia altered the above responses to acute volume loading. Bilateral section of the ansa subclavia (total cardiac sympathectomy) significantly reduced the heart rate response from 35 +/- 5 to 20 +/- 5 beats/min but did not alter other changes. A similar reduction in heart rate response was observed following selective section of the right ansa subclavia. Intravenous infusions of epinephrine augmented the heart rate response in both normally innervated and cardiac sympathectomized dogs. It is suggested that although the primary efferent pathway for the reflex trachycardia is via the vagus, responses are modulated by sympathetic neural activity. Additionally, the nervous system was not shown to play a measureable role in the observed peripheral resistance changes.", "PMID": 970467}
{"id": "pubmed23n0032_8015", "title": "Lipoprotein lipase and uptake of chylomicron triglyceride by skeletal muscle of rats.", "content": "Uptake of chylomicron triglyceride and lipoprotein lipase was studied in red and white skeletal muscles, heart, and adipose tissue of rats. Retention of triglyceride fatty acids 10 min after injection was 1.6%/g in heart and adipose tissue, 0.2-0.4%/g in red (soleus and diaphragm) muscles, and 0.1%/g in white (psoas minor) muscles of fed rats. Fasting (24 h) increased retention two- to fourfold in red skeletal muscles and heart, had no effect in white muscles, and decreased retention greater than 75% in adipose tissue. Lipoprotein lipase activity in fed rats was lowest in white muscles and in certain red (posterior belly of digastric and medial head of triceps brachii) musclws, intermediate in soleus and diaphragm muscles and adipose tissue, and highest in heart. Fasting doubled lipoprotein lipase activity in all red skeletal muscles and heart, had no effect in white muscles, and decreased activity 60% in adipose tissue. The findings indicate that triglyceride uptake is related to lipoprotein lipase activity in skeletal muscle and the changes in enzyme activity during fasting divert blood triglyceride to red skeletal muscles.", "contents": "Lipoprotein lipase and uptake of chylomicron triglyceride by skeletal muscle of rats. Uptake of chylomicron triglyceride and lipoprotein lipase was studied in red and white skeletal muscles, heart, and adipose tissue of rats. Retention of triglyceride fatty acids 10 min after injection was 1.6%/g in heart and adipose tissue, 0.2-0.4%/g in red (soleus and diaphragm) muscles, and 0.1%/g in white (psoas minor) muscles of fed rats. Fasting (24 h) increased retention two- to fourfold in red skeletal muscles and heart, had no effect in white muscles, and decreased retention greater than 75% in adipose tissue. Lipoprotein lipase activity in fed rats was lowest in white muscles and in certain red (posterior belly of digastric and medial head of triceps brachii) musclws, intermediate in soleus and diaphragm muscles and adipose tissue, and highest in heart. Fasting doubled lipoprotein lipase activity in all red skeletal muscles and heart, had no effect in white muscles, and decreased activity 60% in adipose tissue. The findings indicate that triglyceride uptake is related to lipoprotein lipase activity in skeletal muscle and the changes in enzyme activity during fasting divert blood triglyceride to red skeletal muscles.", "PMID": 970468}
{"id": "pubmed23n0032_8016", "title": "Duodenal and ileal adaptation to dietary calcium restriction: in vivo studies in the rat.", "content": "Intestinal adaptation by the growing rat to a low-calcium diet was studied by in situ perfusion of duodenum and ileum in vivo. Rats were fed diets containing either 1.2 or 0.02% Ca for 17-24 days. To study plasma-to-lumen flux and net calcium absorption, rats were loaded parenterally with 45Ca and perfused intraluminally with 3.4 mM calcium. Calcium restriction caused net absorption in ileum to increase fourfold, in duodenum almost twofold. With calcium restriction, plasma-to-lumen flux decreased in duodenum but not in ileum and was small relative to absorption. However, net calcium secretion, when measured in a separate set of animals by intraluminal perfusion of NaCl, decreased in ileum but not in duodenum in response to calcium restriction. The magnitude of adaptation is greater in ileum than duodenum and is largely the result of increased lumen-to-plasma flux. The distal small intestine is probably crucial for calcium homeostasis in dietary calcium deficiency.", "contents": "Duodenal and ileal adaptation to dietary calcium restriction: in vivo studies in the rat. Intestinal adaptation by the growing rat to a low-calcium diet was studied by in situ perfusion of duodenum and ileum in vivo. Rats were fed diets containing either 1.2 or 0.02% Ca for 17-24 days. To study plasma-to-lumen flux and net calcium absorption, rats were loaded parenterally with 45Ca and perfused intraluminally with 3.4 mM calcium. Calcium restriction caused net absorption in ileum to increase fourfold, in duodenum almost twofold. With calcium restriction, plasma-to-lumen flux decreased in duodenum but not in ileum and was small relative to absorption. However, net calcium secretion, when measured in a separate set of animals by intraluminal perfusion of NaCl, decreased in ileum but not in duodenum in response to calcium restriction. The magnitude of adaptation is greater in ileum than duodenum and is largely the result of increased lumen-to-plasma flux. The distal small intestine is probably crucial for calcium homeostasis in dietary calcium deficiency.", "PMID": 970469}
{"id": "pubmed23n0032_8017", "title": "Renal gluconeogenesis and increased glucose utilization in shock.", "content": "The roles of renal gluconeogenesis and glucose utilization in control, hemorrhaged, and endotoxin-injected animals were investigated using anesthetized, eviscerated, nonnephrectomized and nephrectomized dogs. Results demonstrate an increased glucose utilization in both hemorrhagic and endotoxic shock which was marked after endotoxin. Since blood glucose values dropped more in nephrectomized, hemorrhaged animals, in contrast to the nonnephrectomized, hemorrhaged dogs, the kidneys were assumed to perform a significant gluconeogenic role. The kidneys did not appear to perform gluconeogenesis in endotoxin shock since blood glucose levels were comparable in eviscerated, endotoxin-treated animals whether nephrectomized or not. To ascertain the tissue responsible for the increased glucose utilization in endotoxin shock, a study was performed with endotoxin added to blood in vitro (estimated LD100 concentration). The endotoxin-treated blood (n = 7) demonstrated an increased glucose utilization compared with saline controls (n = 7) (P less than or equal 0.02). Acclerated glucose utilization rates were comparable between the eviscerated, nephrectomized animals and in vitro experiments. These data suggest that excessive glucose demand by certain blood components may partially explain the lethal hypoglycemia of endotoxin shock.", "contents": "Renal gluconeogenesis and increased glucose utilization in shock. The roles of renal gluconeogenesis and glucose utilization in control, hemorrhaged, and endotoxin-injected animals were investigated using anesthetized, eviscerated, nonnephrectomized and nephrectomized dogs. Results demonstrate an increased glucose utilization in both hemorrhagic and endotoxic shock which was marked after endotoxin. Since blood glucose values dropped more in nephrectomized, hemorrhaged animals, in contrast to the nonnephrectomized, hemorrhaged dogs, the kidneys were assumed to perform a significant gluconeogenic role. The kidneys did not appear to perform gluconeogenesis in endotoxin shock since blood glucose levels were comparable in eviscerated, endotoxin-treated animals whether nephrectomized or not. To ascertain the tissue responsible for the increased glucose utilization in endotoxin shock, a study was performed with endotoxin added to blood in vitro (estimated LD100 concentration). The endotoxin-treated blood (n = 7) demonstrated an increased glucose utilization compared with saline controls (n = 7) (P less than or equal 0.02). Acclerated glucose utilization rates were comparable between the eviscerated, nephrectomized animals and in vitro experiments. These data suggest that excessive glucose demand by certain blood components may partially explain the lethal hypoglycemia of endotoxin shock.", "PMID": 970470}
{"id": "pubmed23n0032_8018", "title": "Effect of fatty acids on renal ammoniagenesis in in vivo and in vitro studies.", "content": "Intravenous or renal intra-arterial infusion of sodium octanoate results in a 60% decrease in renal ammoniagenesis in the acidotic dog. Mobilization of endogenous fatty acids by levarterenol infusion is accompanied by a 30% fall in renal ammoniagenesis which coincides with considerable increase in renal extraction of fatty acids. In both types of experiments, the renal extraction of glutamine falls in proportion with decreased ammoniagenesis. The effect of octanoate and levarterenol infusion cannot be explained by changes in acid-base equilibrium, renal hemodynamics, or the release of insulin. In vitro experiments using kidney cortical slices from acidotic dogs show that addition of sodium octanoate (0.05-10 mM) or sodium palmitate (0.1-2.5 mM) to the incubation medium induces a 35% decrease in both ammonia and glucose production when L-glutamine (1 mM) is used as the basic ammoniagenic and gluconeogenic substrate. Glutamine uptake decreases concomitantly, whereas tissue glutamate either rises or remains unchanged. The same results were observed when L-glutamate (5 mM) was used as substrate. Glycerol (5 mM) in the medium has no effect on ammoniagenesis, whereas gluconeogenesis increases by 81%. The present studies demonstrate that fatty acids may interfere with renal ammoniagenesis from glutamine during acidosis. The effect is probably related to substrate availability and competition. Fatty acids appear to inhibit ammoniagenesis in the mitochondria through a direct metabolic effect linked with their oxidation and not through modification of glutamine transport across the mitochondrial membrane.", "contents": "Effect of fatty acids on renal ammoniagenesis in in vivo and in vitro studies. Intravenous or renal intra-arterial infusion of sodium octanoate results in a 60% decrease in renal ammoniagenesis in the acidotic dog. Mobilization of endogenous fatty acids by levarterenol infusion is accompanied by a 30% fall in renal ammoniagenesis which coincides with considerable increase in renal extraction of fatty acids. In both types of experiments, the renal extraction of glutamine falls in proportion with decreased ammoniagenesis. The effect of octanoate and levarterenol infusion cannot be explained by changes in acid-base equilibrium, renal hemodynamics, or the release of insulin. In vitro experiments using kidney cortical slices from acidotic dogs show that addition of sodium octanoate (0.05-10 mM) or sodium palmitate (0.1-2.5 mM) to the incubation medium induces a 35% decrease in both ammonia and glucose production when L-glutamine (1 mM) is used as the basic ammoniagenic and gluconeogenic substrate. Glutamine uptake decreases concomitantly, whereas tissue glutamate either rises or remains unchanged. The same results were observed when L-glutamate (5 mM) was used as substrate. Glycerol (5 mM) in the medium has no effect on ammoniagenesis, whereas gluconeogenesis increases by 81%. The present studies demonstrate that fatty acids may interfere with renal ammoniagenesis from glutamine during acidosis. The effect is probably related to substrate availability and competition. Fatty acids appear to inhibit ammoniagenesis in the mitochondria through a direct metabolic effect linked with their oxidation and not through modification of glutamine transport across the mitochondrial membrane.", "PMID": 970471}
{"id": "pubmed23n0032_8019", "title": "Interstitial fluid oncotic pressures in rabbit subcutaneous tissue.", "content": "Interstitial fluid oncotic pressures in rabbit subcutaneous tissues were measured by chronically implanted membrane osmometers. Pairs of osmometers were used, one using a membrane permeable and one impermeable to plasma proteins. Measurements from the small-pore membranes averaged 10.2 mmHg while pressures measured from the large-pore membrane osmometers averaged -1.2 mmHg, indicating that the measured pressures were osmotic rather than hydrostatic in nature. These values are in agreement with previously published values from this laboratory on oncotic pressures of excised rabbit skin and with a computer-simulation study of capillary fluid balance. The oncotic pressures described in this study are of the s-me general magnitude as those of lymph from most organs in the body.", "contents": "Interstitial fluid oncotic pressures in rabbit subcutaneous tissue. Interstitial fluid oncotic pressures in rabbit subcutaneous tissues were measured by chronically implanted membrane osmometers. Pairs of osmometers were used, one using a membrane permeable and one impermeable to plasma proteins. Measurements from the small-pore membranes averaged 10.2 mmHg while pressures measured from the large-pore membrane osmometers averaged -1.2 mmHg, indicating that the measured pressures were osmotic rather than hydrostatic in nature. These values are in agreement with previously published values from this laboratory on oncotic pressures of excised rabbit skin and with a computer-simulation study of capillary fluid balance. The oncotic pressures described in this study are of the s-me general magnitude as those of lymph from most organs in the body.", "PMID": 970472}
{"id": "pubmed23n0032_8020", "title": "Stimulation of rat lung metabolism with 2,4-dinitrophenol and phenazine methosulfate.", "content": "Glucose metabolism was stimulated in isolated perfused rat lungs by perfusion with 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation or phenazine methosulfate (PMS), an artificial hydrogen acceptor. Lungs were ventilated with 95% O2:5% CO2 and perfused with Krebs-Ringer bicarbonate buffer pH 7.4 containing 5.5 mM [U-14C, 5-3H]glucose for 100 min. In control lungs, 45% of total glucose 14C was recovered as perfusate lactate plus pyruvate (L+P) and 24% as 14CO2. Perfuson with 0.8 mM DNP resulted in a 102% increase in 14CO2 production and a 98% increase in L+P, associated with a fall in tissue ATP/ADP ratio and decreased incorporation of glucose carbons into lipids. Perfusion with 8 muM PMS resulted in a 116% increase in 14CO2 production but no change in L+P; the lactate-to-pyruvate ratio in the perfusate was reduced to 4 from a control value of 10, and tissue adenine nucleotide levels were unchanged. In all experiments, 3H was mainly recovered as 3H2O in the perfusate. These data demonstrate the pattern of response of the isolated lung preparation to uncoupling of oxidative phosphorylation and to alterations in cytoplasmic redox state. The results suggest that the isolated lung preparation is metabolically intact and suitable for study of metabolic control processes.", "contents": "Stimulation of rat lung metabolism with 2,4-dinitrophenol and phenazine methosulfate. Glucose metabolism was stimulated in isolated perfused rat lungs by perfusion with 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation or phenazine methosulfate (PMS), an artificial hydrogen acceptor. Lungs were ventilated with 95% O2:5% CO2 and perfused with Krebs-Ringer bicarbonate buffer pH 7.4 containing 5.5 mM [U-14C, 5-3H]glucose for 100 min. In control lungs, 45% of total glucose 14C was recovered as perfusate lactate plus pyruvate (L+P) and 24% as 14CO2. Perfuson with 0.8 mM DNP resulted in a 102% increase in 14CO2 production and a 98% increase in L+P, associated with a fall in tissue ATP/ADP ratio and decreased incorporation of glucose carbons into lipids. Perfusion with 8 muM PMS resulted in a 116% increase in 14CO2 production but no change in L+P; the lactate-to-pyruvate ratio in the perfusate was reduced to 4 from a control value of 10, and tissue adenine nucleotide levels were unchanged. In all experiments, 3H was mainly recovered as 3H2O in the perfusate. These data demonstrate the pattern of response of the isolated lung preparation to uncoupling of oxidative phosphorylation and to alterations in cytoplasmic redox state. The results suggest that the isolated lung preparation is metabolically intact and suitable for study of metabolic control processes.", "PMID": 970473}
{"id": "pubmed23n0032_8021", "title": "Energy expenditure for thermoregulation and locomotion in emperor penguins.", "content": "During the antarctic winter emperor penguins (Aptenodytes forsteri) spend up to four mo fasting while they breed at rookeries 80 km or more from the sea, huddling close together in the cold. This breeding cycle makes exceptional demands on their energy reserves, and we therefore studied their thermoregulation and locomotion. Rates of metabolism were measured in five birds (mean body mass, 23.37 kg) at ambient temperatures ranging from 25 to -47 degrees C. Between 20 and -10 degrees C the metabolic rate (standard metabolic rate (SMR)) remained neraly constant, about 42.9 W. Below -10 degrees C metabolic rate increased lineraly with decreasing ambient temperature and at -47 degrees C it was 70% above the SMR. Mean thermal conductance below -10 degrees C was 1.57 W m-2 degrees C-1. Metabolic rate during treadmill walking increased linearly with increasing speed. Our data suggest that walking 200 km (from the sea to the rookery and back) requires less than 15% of the energy reserves of a breeding male emperor penguin initially weighing 35 kg. The high energy requirement for thermoregulation (about 85%) would, in the absence of huddling, probably exceed the total energy reserves.", "contents": "Energy expenditure for thermoregulation and locomotion in emperor penguins. During the antarctic winter emperor penguins (Aptenodytes forsteri) spend up to four mo fasting while they breed at rookeries 80 km or more from the sea, huddling close together in the cold. This breeding cycle makes exceptional demands on their energy reserves, and we therefore studied their thermoregulation and locomotion. Rates of metabolism were measured in five birds (mean body mass, 23.37 kg) at ambient temperatures ranging from 25 to -47 degrees C. Between 20 and -10 degrees C the metabolic rate (standard metabolic rate (SMR)) remained neraly constant, about 42.9 W. Below -10 degrees C metabolic rate increased lineraly with decreasing ambient temperature and at -47 degrees C it was 70% above the SMR. Mean thermal conductance below -10 degrees C was 1.57 W m-2 degrees C-1. Metabolic rate during treadmill walking increased linearly with increasing speed. Our data suggest that walking 200 km (from the sea to the rookery and back) requires less than 15% of the energy reserves of a breeding male emperor penguin initially weighing 35 kg. The high energy requirement for thermoregulation (about 85%) would, in the absence of huddling, probably exceed the total energy reserves.", "PMID": 970474}
{"id": "pubmed23n0032_8022", "title": "Thermoregulation in fasting emperor penguins under natural conditions.", "content": "Emperor penguins breed during the cold antarctic winter. The males incubate the single egg while fasting for up to 4 mo and losing some 20 kg of their body mass. Fasting captive birds under outdoor conditions lost from 0.145 to 0.434 kg day -1. Mean resting metabolic rate, 49.06 W for 24.8 kg body mass, is 7 and 27%, respectively, higher than predicted from general metabolic equations for birds. Minimal thermal conductance, 1.31 W m-2 degrees C-1, is within the range for other birds. The lower critical temperature is about -10 degrees C; this can be related to large body size (20-40 kg) and to body shape, giving a smaller relative surface area than for other birds. Rigidity of the feathers explains why winds of moderate speed (up to 5 m s-1) have little effect on heat loss. At very low temperatures the behavior of huddling close together is essential in reducing metabolic rate. Without this behavior, survival during the long fast (up to four mo) at winter temperatures would be impossible.", "contents": "Thermoregulation in fasting emperor penguins under natural conditions. Emperor penguins breed during the cold antarctic winter. The males incubate the single egg while fasting for up to 4 mo and losing some 20 kg of their body mass. Fasting captive birds under outdoor conditions lost from 0.145 to 0.434 kg day -1. Mean resting metabolic rate, 49.06 W for 24.8 kg body mass, is 7 and 27%, respectively, higher than predicted from general metabolic equations for birds. Minimal thermal conductance, 1.31 W m-2 degrees C-1, is within the range for other birds. The lower critical temperature is about -10 degrees C; this can be related to large body size (20-40 kg) and to body shape, giving a smaller relative surface area than for other birds. Rigidity of the feathers explains why winds of moderate speed (up to 5 m s-1) have little effect on heat loss. At very low temperatures the behavior of huddling close together is essential in reducing metabolic rate. Without this behavior, survival during the long fast (up to four mo) at winter temperatures would be impossible.", "PMID": 970475}
{"id": "pubmed23n0032_8023", "title": "Effect of dorsal root section on the arrhythmias associated with coronary occlusion.", "content": "In anesthetized vagotomized dogs and cats the circumflex and/or the anterior descending coronary artery were briefly occluded (5-90 s), and ectopic beats occurring during the occlusion and for 60 s following release were counted. When arrhythmias were regularly produced for a given occlusion, the dorsal roots from C8 to T5 were transected and the occlusions were repeated. Dorsal root section produced minor changes in heart rate and blood pressure. Dogs and cats did not differ in their responses. Dorsal root section was performed in eight animals and decreased the absolute number of ectopic beats by 63 +/- 19% compared to control values (P less than 0.05). In four animals the effect on ectopic beats produced by repeated occlusions without dorsal root section was investigated and found to be increased by 35 +/- 24% compared to contrl values. Most of the somatic afferents contained in the dorsal roots were damaged by the surgical preparation. Therefore, repeated occlusions and interruption of somatic afferents do not appear to have influenced our results. The arrhythmogenic interaction between the local effects of myocardial ischemia and the sympathetic activity, whose outlow contained in the ventral roots remained intact, was still possible after dorsal root section and this explains why ectopic beats were reduced but not almost suppressed as is usually the case after bilateral stellectomy. We conclude that dorsal root section reduces the number of ectopic beats associated with short-lasting coronary artery occlusions and that the most likely mechanism is the interruption of the cardiocardiac sympathetic reflex which depends upon afferent fibers running through the dorsal roots.", "contents": "Effect of dorsal root section on the arrhythmias associated with coronary occlusion. In anesthetized vagotomized dogs and cats the circumflex and/or the anterior descending coronary artery were briefly occluded (5-90 s), and ectopic beats occurring during the occlusion and for 60 s following release were counted. When arrhythmias were regularly produced for a given occlusion, the dorsal roots from C8 to T5 were transected and the occlusions were repeated. Dorsal root section produced minor changes in heart rate and blood pressure. Dogs and cats did not differ in their responses. Dorsal root section was performed in eight animals and decreased the absolute number of ectopic beats by 63 +/- 19% compared to control values (P less than 0.05). In four animals the effect on ectopic beats produced by repeated occlusions without dorsal root section was investigated and found to be increased by 35 +/- 24% compared to contrl values. Most of the somatic afferents contained in the dorsal roots were damaged by the surgical preparation. Therefore, repeated occlusions and interruption of somatic afferents do not appear to have influenced our results. The arrhythmogenic interaction between the local effects of myocardial ischemia and the sympathetic activity, whose outlow contained in the ventral roots remained intact, was still possible after dorsal root section and this explains why ectopic beats were reduced but not almost suppressed as is usually the case after bilateral stellectomy. We conclude that dorsal root section reduces the number of ectopic beats associated with short-lasting coronary artery occlusions and that the most likely mechanism is the interruption of the cardiocardiac sympathetic reflex which depends upon afferent fibers running through the dorsal roots.", "PMID": 970476}
{"id": "pubmed23n0032_8024", "title": "Stretch receptor model for renin release with evidence from perfused rat kidney.", "content": "Control of renin release was studied in isolated rat kidneys perfused with Krebs-Henseleit solution containing albumin. Cumulative perfusate renin activity (PRA) as measured by radioimmunoassay was increased by low perfusion pressure and suppressed by high pressure. Renal vasoconstriction induced by infusion of phenylephrine or methoxamine increased PRA, whereas vasodilatation by papaverine suppressed renin activity. The increased renin activity induced by phenylephrine was blocked by high pressure or papaverine. Changing sodium concentration in the perfusion medium had no effect on basal renin release. A mathematical analysis for an intrarenal stretch receptor indicates that renin release is related to the elastic modulus, the internal and external hydrostatic pressures, and the internal and external radii. Calculations based on this model also indicate that renin release is most sensitive to changes in the ratio of the radii. It is proposed that basodilatation or high perfusion pressure may increase the stretch of the afferent arteriole and depolarize the granular cell membrane, whereas vasoconstriction or low pressure may decrease stretch and thus hyperpolarize the cell.", "contents": "Stretch receptor model for renin release with evidence from perfused rat kidney. Control of renin release was studied in isolated rat kidneys perfused with Krebs-Henseleit solution containing albumin. Cumulative perfusate renin activity (PRA) as measured by radioimmunoassay was increased by low perfusion pressure and suppressed by high pressure. Renal vasoconstriction induced by infusion of phenylephrine or methoxamine increased PRA, whereas vasodilatation by papaverine suppressed renin activity. The increased renin activity induced by phenylephrine was blocked by high pressure or papaverine. Changing sodium concentration in the perfusion medium had no effect on basal renin release. A mathematical analysis for an intrarenal stretch receptor indicates that renin release is related to the elastic modulus, the internal and external hydrostatic pressures, and the internal and external radii. Calculations based on this model also indicate that renin release is most sensitive to changes in the ratio of the radii. It is proposed that basodilatation or high perfusion pressure may increase the stretch of the afferent arteriole and depolarize the granular cell membrane, whereas vasoconstriction or low pressure may decrease stretch and thus hyperpolarize the cell.", "PMID": 970477}
{"id": "pubmed23n0032_8025", "title": "Effectiveness of the aldosterone-sodium and -potassium feedback control system.", "content": "This study was conducted to determine the quantitative importance of the aldosterone feedback mechanism in controlling each one of three major factors that have often been associated with aldosterone, namely, extracellular fluid sodium concentration, extracellular fluid potassium concentration, and extracellular fluid volume. To do this, the ability of the body to control these three factors in the face of marked changes in daily sodium or potassium intake was studied under two conditions: 1) in the normal dog, and 2) in the dog in which the aldosterone feedback mechanism was prevented from functioning by removing the adrenal glands and then providing a continuous fixed level of supportive aldosterone and glucocorticoids during the low and high electrolyte intake periods. Under these conditions, removal of feedback control of aldosterone secretion decreased the effectiveness of plasma potassium control by nearly fivefold (39% vs. 8% change in plasma potassium concentration), fluid volume by sixfold (12% vs. 2% change in sodium space) and had no effect on control of plasma sodium concentration (2% change with and without feedback control of aldosterone secretion.)", "contents": "Effectiveness of the aldosterone-sodium and -potassium feedback control system. This study was conducted to determine the quantitative importance of the aldosterone feedback mechanism in controlling each one of three major factors that have often been associated with aldosterone, namely, extracellular fluid sodium concentration, extracellular fluid potassium concentration, and extracellular fluid volume. To do this, the ability of the body to control these three factors in the face of marked changes in daily sodium or potassium intake was studied under two conditions: 1) in the normal dog, and 2) in the dog in which the aldosterone feedback mechanism was prevented from functioning by removing the adrenal glands and then providing a continuous fixed level of supportive aldosterone and glucocorticoids during the low and high electrolyte intake periods. Under these conditions, removal of feedback control of aldosterone secretion decreased the effectiveness of plasma potassium control by nearly fivefold (39% vs. 8% change in plasma potassium concentration), fluid volume by sixfold (12% vs. 2% change in sodium space) and had no effect on control of plasma sodium concentration (2% change with and without feedback control of aldosterone secretion.)", "PMID": 970478}
{"id": "pubmed23n0032_8026", "title": "Incidence and pathophysiological changes in chronic two-kidney hypertension in the dog.", "content": "Unilateral renal artery plication in dogs reduced renal blood flow by 80% and produced a sustained elevation in arterial pressure whereas plasma renin activity increased for only 4 days. Sodium was retained for 3 days after plication, but this response is similar to that after a sham operation. Of seven dogs studied chronically, elevated arterial pressure was sustained for 27 days or longer in six animals. In three dogs hypertension continued for 2 mo before collateral vessels developed and arterial pressure fell; ligation of these collaterals restored hypertension. Arterial pressure was unaffected by an infusion of [1-sarcosine, 8-alanine] angiotensin II in chronic hypertensive dogs on a normal sodium intake. This angiotensin antagonist lowered arterial pressure after sodium depletion, but became ineffective following rapid sodium repletion. Chronic hypertensive dogs showed normal responses to deoxycorticosterone acetate. These findings suggest that the renin-angiotensin system is not critically involved in maintenace of chronic two-kidney renovascular hypertension in the dog. The data also show that the homeostatic role played by the renin-angiotensin system in the maintenance of arterial pressure remained intact in chronic hypertension.", "contents": "Incidence and pathophysiological changes in chronic two-kidney hypertension in the dog. Unilateral renal artery plication in dogs reduced renal blood flow by 80% and produced a sustained elevation in arterial pressure whereas plasma renin activity increased for only 4 days. Sodium was retained for 3 days after plication, but this response is similar to that after a sham operation. Of seven dogs studied chronically, elevated arterial pressure was sustained for 27 days or longer in six animals. In three dogs hypertension continued for 2 mo before collateral vessels developed and arterial pressure fell; ligation of these collaterals restored hypertension. Arterial pressure was unaffected by an infusion of [1-sarcosine, 8-alanine] angiotensin II in chronic hypertensive dogs on a normal sodium intake. This angiotensin antagonist lowered arterial pressure after sodium depletion, but became ineffective following rapid sodium repletion. Chronic hypertensive dogs showed normal responses to deoxycorticosterone acetate. These findings suggest that the renin-angiotensin system is not critically involved in maintenace of chronic two-kidney renovascular hypertension in the dog. The data also show that the homeostatic role played by the renin-angiotensin system in the maintenance of arterial pressure remained intact in chronic hypertension.", "PMID": 970479}
{"id": "pubmed23n0032_8027", "title": "Preglomerular resistance and glomerular perfusion in the rat and dog.", "content": "Glomerular blood flow in the rat, measured with radioactive microspheres, averaged 233 +/- 59 nl/min per glomerulus, significantly less than the glomerular flow rate in the dog (568 +/- 115; P less than 0.005). The difference in glomerular blood flow rate could not be attributed to differences in mean or cortical flow rates, the fraction of acrdiac output received, cardiac output normalized to body weight, or the fractional distribution of blood flow or glomeruli from outer to inner cortex in the two species. The size of microspheres reaching the glomerulus, however, was significantly larger in the dog than in the rat (P less than 0.0005) suggesting that afferent arterioles were larger in the dog than rat. The difference in afferent resistance calculated from the size of microspheres delivered to the glomeruli was larger than the difference in glomerular blood flow. With a similar arterial pressure, a lower afferent resistance suggests a higher glomerular capillary pressure in the dog, consistent with a number of suggestions that filtration equilibrium is less likely in this species.", "contents": "Preglomerular resistance and glomerular perfusion in the rat and dog. Glomerular blood flow in the rat, measured with radioactive microspheres, averaged 233 +/- 59 nl/min per glomerulus, significantly less than the glomerular flow rate in the dog (568 +/- 115; P less than 0.005). The difference in glomerular blood flow rate could not be attributed to differences in mean or cortical flow rates, the fraction of acrdiac output received, cardiac output normalized to body weight, or the fractional distribution of blood flow or glomeruli from outer to inner cortex in the two species. The size of microspheres reaching the glomerulus, however, was significantly larger in the dog than in the rat (P less than 0.0005) suggesting that afferent arterioles were larger in the dog than rat. The difference in afferent resistance calculated from the size of microspheres delivered to the glomeruli was larger than the difference in glomerular blood flow. With a similar arterial pressure, a lower afferent resistance suggests a higher glomerular capillary pressure in the dog, consistent with a number of suggestions that filtration equilibrium is less likely in this species.", "PMID": 970480}
{"id": "pubmed23n0032_8028", "title": "Uptake and release of hormones and metabolites by tissues of exercising leg in man.", "content": "To examine release of insulin from tissues of the exercising llin, growth hormone, cortisol, and circulating metabolites were studied in five men before, during, and after exercise on a bicycle ergometer at 60% of their maximum work capacity. At rest, insulin, growth hormone, and cortisol were taken up by leg tissues. During exercise arterial plasma insulin concentration fell, but cortisol and growth hormone levels rose; there was net release of insulin into venous blood but little change in uptake of cortisol and growth hormone. Insulin release persisted after exercise for 15-30 min. During exercise arterial concentrations and uptake of glucose and free fatty acids (FFA) increased. Examination of the changes in hormones and metabolites failed to identify any single hormonal or metabolic factor causing the observed reversal of insulin uptake with exercise.", "contents": "Uptake and release of hormones and metabolites by tissues of exercising leg in man. To examine release of insulin from tissues of the exercising llin, growth hormone, cortisol, and circulating metabolites were studied in five men before, during, and after exercise on a bicycle ergometer at 60% of their maximum work capacity. At rest, insulin, growth hormone, and cortisol were taken up by leg tissues. During exercise arterial plasma insulin concentration fell, but cortisol and growth hormone levels rose; there was net release of insulin into venous blood but little change in uptake of cortisol and growth hormone. Insulin release persisted after exercise for 15-30 min. During exercise arterial concentrations and uptake of glucose and free fatty acids (FFA) increased. Examination of the changes in hormones and metabolites failed to identify any single hormonal or metabolic factor causing the observed reversal of insulin uptake with exercise.", "PMID": 970481}
{"id": "pubmed23n0032_8029", "title": "Changes in canine saliva ion concentration induced by increased intraluminal pressure.", "content": "Hydrostatic pressure was applied to a cannula inserted into Wharton's duct of adult dogs anesthetized with pentobarbital and the effect of pressure on saliva flow rate and ionic composition of saliva was measured. Increasing the back pressure on a secreting gland resulted in a decrease in flow rate. Over the range of 0-100 mmHg back pressure the decrease in flow was proportional to the applied back pressure. The potassium concentration of saliva collected in the absence of back pressure and at raised pressure was similar even though back pressure reduced flow. In contrast, sodium concentration decreased when back pressure caused salivary flow rate to be reduced. However, when examined at the same flow rates the concentration of sodium is increased when back pressure is applied to the gland as compared to absence of back pressure. Under conditions of constant stimulation the total output of sodium and potassium into saliva decreases as back pressure is increased; but when equal flow rates are compared total sodium output increases in the presence of applied back pressure, whereas potassium output remains constant.", "contents": "Changes in canine saliva ion concentration induced by increased intraluminal pressure. Hydrostatic pressure was applied to a cannula inserted into Wharton's duct of adult dogs anesthetized with pentobarbital and the effect of pressure on saliva flow rate and ionic composition of saliva was measured. Increasing the back pressure on a secreting gland resulted in a decrease in flow rate. Over the range of 0-100 mmHg back pressure the decrease in flow was proportional to the applied back pressure. The potassium concentration of saliva collected in the absence of back pressure and at raised pressure was similar even though back pressure reduced flow. In contrast, sodium concentration decreased when back pressure caused salivary flow rate to be reduced. However, when examined at the same flow rates the concentration of sodium is increased when back pressure is applied to the gland as compared to absence of back pressure. Under conditions of constant stimulation the total output of sodium and potassium into saliva decreases as back pressure is increased; but when equal flow rates are compared total sodium output increases in the presence of applied back pressure, whereas potassium output remains constant.", "PMID": 970482}
{"id": "pubmed23n0032_8030", "title": "A reconsideration of the Kety and associates study of genetic factors in the transmission of schizophrenia.", "content": "The author reviews a study by Kety and associates that reported a significantly greater prevalence of schizophrenic spectrum disorders among the biological relatives of schizophrenic adoptees than among those of nonschizophrenic adoptees. The  principal statistical analysis of the Kety study used an incorrect sample size (306 rather than 66) and failed to weight each index and control case (family) equally. This violation of the independence assumption would allow a few families to disproportionately influence the outcome. The author argues that proper statistical analysis applied separately to available categories indicates that significant differences between the index and control groups occurred mainly in the half-sibling category; this result violates the principle that genetic effects increase with greater consanguinity. She concludes that Kety and associates' study raises more questions than it answers regarding the role of genetic factors in schizophrenia.", "contents": "A reconsideration of the Kety and associates study of genetic factors in the transmission of schizophrenia. The author reviews a study by Kety and associates that reported a significantly greater prevalence of schizophrenic spectrum disorders among the biological relatives of schizophrenic adoptees than among those of nonschizophrenic adoptees. The  principal statistical analysis of the Kety study used an incorrect sample size (306 rather than 66) and failed to weight each index and control case (family) equally. This violation of the independence assumption would allow a few families to disproportionately influence the outcome. The author argues that proper statistical analysis applied separately to available categories indicates that significant differences between the index and control groups occurred mainly in the half-sibling category; this result violates the principle that genetic effects increase with greater consanguinity. She concludes that Kety and associates' study raises more questions than it answers regarding the role of genetic factors in schizophrenia.", "PMID": 970483}
{"id": "pubmed23n0032_8031", "title": "Studies designed to disentangle genetic and environmental variables in schizophrenia: some epistemological questions and answers.", "content": "The author addresses questions raised by Dr. Lorna S. Benjamin in the preceding paper. He quotes from the original studies to respond to questions regarding statistical logic, the relative prevalence of schizophrenia among half-siblings and parents of index adoptees, and the possible role of socioeconomic status as a confounding variable.", "contents": "Studies designed to disentangle genetic and environmental variables in schizophrenia: some epistemological questions and answers. The author addresses questions raised by Dr. Lorna S. Benjamin in the preceding paper. He quotes from the original studies to respond to questions regarding statistical logic, the relative prevalence of schizophrenia among half-siblings and parents of index adoptees, and the possible role of socioeconomic status as a confounding variable.", "PMID": 970484}
{"id": "pubmed23n0032_8032", "title": "The legal implications of sexual activity between psychiatrist and patient.", "content": "The author examines the legal and professional sanctions against sex between therapist and patient. The relevant literature of criminal law suggests that charges of rape or related sexual offenses against psychotherapists who exploit their patients are a remote possibility. Although sexual activity between therapist and patient may form the  basis of a malpractice claim, the existing case law is complicated, and the legal distinctions are not always consistent with good psychiatric practice. Professional associations lack the legal expertise and indemnification to act in these situations, but medical boards in some states have the power to revoke licenses. In the end, patients must depend on the decent moral character of their therapists.", "contents": "The legal implications of sexual activity between psychiatrist and patient. The author examines the legal and professional sanctions against sex between therapist and patient. The relevant literature of criminal law suggests that charges of rape or related sexual offenses against psychotherapists who exploit their patients are a remote possibility. Although sexual activity between therapist and patient may form the  basis of a malpractice claim, the existing case law is complicated, and the legal distinctions are not always consistent with good psychiatric practice. Professional associations lack the legal expertise and indemnification to act in these situations, but medical boards in some states have the power to revoke licenses. In the end, patients must depend on the decent moral character of their therapists.", "PMID": 970485}
{"id": "pubmed23n0032_8033", "title": "The alcoholic physician: a survey.", "content": "The authors conducted interviews with 98 recovered alcoholic physicians, all of whom had been entirely abstinent for a minimum of one calendar year. Psychiatry was the only specialty clearly overrepresented. A disproportionate number of subjects reported high standing in their medical school classes. Nearly half of the sample had experienced difficulty with drugs other than alcohol. While legal sanctions and admission to health care and correctional institutions were common among this group, relatively little formal response of a disciplinary nature from colleagues or medical organizations was reported.", "contents": "The alcoholic physician: a survey. The authors conducted interviews with 98 recovered alcoholic physicians, all of whom had been entirely abstinent for a minimum of one calendar year. Psychiatry was the only specialty clearly overrepresented. A disproportionate number of subjects reported high standing in their medical school classes. Nearly half of the sample had experienced difficulty with drugs other than alcohol. While legal sanctions and admission to health care and correctional institutions were common among this group, relatively little formal response of a disciplinary nature from colleagues or medical organizations was reported.", "PMID": 970486}
{"id": "pubmed23n0032_8034", "title": "Impact of a district branch Task Force on women.", "content": "A questionnaire sent to all women psychiatrists and trainees in North Carolina as part of the development of a district branch Task Force on Women indicated that institutional sexism affects the careers of many of the respondents. Problems of family-professional role conflicts were especially pronounced in residents and faculty women. Survey findings have resulted in recommendations in the areas of education, communication, and increasing women's participation in task force and district branch activities. The authors feel that such task forces provide a much needed network of supportive relationships for women psychiatrists and raise the consciousness of the district branch regarding women's issues.", "contents": "Impact of a district branch Task Force on women. A questionnaire sent to all women psychiatrists and trainees in North Carolina as part of the development of a district branch Task Force on Women indicated that institutional sexism affects the careers of many of the respondents. Problems of family-professional role conflicts were especially pronounced in residents and faculty women. Survey findings have resulted in recommendations in the areas of education, communication, and increasing women's participation in task force and district branch activities. The authors feel that such task forces provide a much needed network of supportive relationships for women psychiatrists and raise the consciousness of the district branch regarding women's issues.", "PMID": 970487}
{"id": "pubmed23n0032_8035", "title": "Importance of dopamine metabolism for clinical effects and side effects of neuroleptics.", "content": "The authors studied the relation between human central dopamine (DA) metabolism and the clinical effects of neuroleptics. The neuroleptic-induced increase in central DA turnover (an indicator for the degree of DA receptor blocking) was found to be positively correlated with the therapeutic effect of neuroleptics and the development of hypokinetic-rigid symptoms. This supplies a direct argument in support of the contention that DA antagonism is related to the occurrence of clinical effects. The authors also found indications that neuroleptics of different chemical types do not significantly differ in their intrinsic ability to provoke hypokinetic-rigid symptoms, that development of these symptoms depends on the patient's individual susceptibility, and that individual susceptibility is based on relatively low DA turnover.", "contents": "Importance of dopamine metabolism for clinical effects and side effects of neuroleptics. The authors studied the relation between human central dopamine (DA) metabolism and the clinical effects of neuroleptics. The neuroleptic-induced increase in central DA turnover (an indicator for the degree of DA receptor blocking) was found to be positively correlated with the therapeutic effect of neuroleptics and the development of hypokinetic-rigid symptoms. This supplies a direct argument in support of the contention that DA antagonism is related to the occurrence of clinical effects. The authors also found indications that neuroleptics of different chemical types do not significantly differ in their intrinsic ability to provoke hypokinetic-rigid symptoms, that development of these symptoms depends on the patient's individual susceptibility, and that individual susceptibility is based on relatively low DA turnover.", "PMID": 970488}
{"id": "pubmed23n0032_8036", "title": "A dopaminergic mechanism in mania.", "content": "The authors present a case study that explores the relationship of dopamine function and manic illness through the use of two drugs with relatively specific effects in stimulating and blocking dopamine receptors--piribedil (ET--495) and pimozide, respectively. Piribedil, as well as d-amphetamine, was associated with manic episodes, while pimozide had an antimanic effect. These observations suggest that dopaminergic mechanisms may be involved in the mediation of manic episodes in at least some patients.", "contents": "A dopaminergic mechanism in mania. The authors present a case study that explores the relationship of dopamine function and manic illness through the use of two drugs with relatively specific effects in stimulating and blocking dopamine receptors--piribedil (ET--495) and pimozide, respectively. Piribedil, as well as d-amphetamine, was associated with manic episodes, while pimozide had an antimanic effect. These observations suggest that dopaminergic mechanisms may be involved in the mediation of manic episodes in at least some patients.", "PMID": 970489}
{"id": "pubmed23n0032_8037", "title": "Platelet monoamine oxidase activity in schizophrenia.", "content": "The activity of platelet monoamine oxidase in 12 chronic schizophrenic patients was not significantly different from that in a matched group of normal individuals. The authors emphasize the importance of simultaneous processing of control and patient blood samples and the use of carefully controlled techniques, since relatively minor changes in procedure can markedly influence platelet yield and leucocyte contamination.", "contents": "Platelet monoamine oxidase activity in schizophrenia. The activity of platelet monoamine oxidase in 12 chronic schizophrenic patients was not significantly different from that in a matched group of normal individuals. The authors emphasize the importance of simultaneous processing of control and patient blood samples and the use of carefully controlled techniques, since relatively minor changes in procedure can markedly influence platelet yield and leucocyte contamination.", "PMID": 970490}
{"id": "pubmed23n0032_8038", "title": "Treatment of requests for sex-change surgery with psychotherapy.", "content": "The authors describe two patients whose requests for sex-change surgery represented crises in sexual identity and anxiety-masking symptoms. Brief psychotherapy enabled these patients to relinquish their belief in a surgical \"cure\". In evaluating such request, the psychiatrist should consider the patient's total personality rather than focusing on the genuineness of the perceived gender disorder. Whatever the final decision, the opportunity for continued psychotherapy should be provided.", "contents": "Treatment of requests for sex-change surgery with psychotherapy. The authors describe two patients whose requests for sex-change surgery represented crises in sexual identity and anxiety-masking symptoms. Brief psychotherapy enabled these patients to relinquish their belief in a surgical \"cure\". In evaluating such request, the psychiatrist should consider the patient's total personality rather than focusing on the genuineness of the perceived gender disorder. Whatever the final decision, the opportunity for continued psychotherapy should be provided.", "PMID": 970491}
{"id": "pubmed23n0032_8039", "title": "Peer counseling in a general hospital.", "content": "Self-help groups can aid an individual in coping with and adapting to catastrophic illness. The authors describe a therapeutic technique in which a member of a medical team serves as a catalyst in introducing a \"veteran\" patient to a newly disabled patient with the same disease or problem so that they can share feelings, experiences, and strategies. Two cases are presented in which patients who were severely depressed benefited from peer counseling. Short-term or \"one-shot\" encounters can be a valuable way to help some disabled patients deal with the anxiety and helplessness they feel in the acute states of serious disease or injury.", "contents": "Peer counseling in a general hospital. Self-help groups can aid an individual in coping with and adapting to catastrophic illness. The authors describe a therapeutic technique in which a member of a medical team serves as a catalyst in introducing a \"veteran\" patient to a newly disabled patient with the same disease or problem so that they can share feelings, experiences, and strategies. Two cases are presented in which patients who were severely depressed benefited from peer counseling. Short-term or \"one-shot\" encounters can be a valuable way to help some disabled patients deal with the anxiety and helplessness they feel in the acute states of serious disease or injury.", "PMID": 970492}
{"id": "pubmed23n0032_8040", "title": "The psychiatric resident as an ex-officio community board member.", "content": "The authors describe a program in which third-year psychiatric residents participate as ex-officio members on the boards of community service agencies. Experience as board members gives residents a broad understanding of issues related to the delivery of community mental health services and encourages them to continue community involvment after their training is completed.", "contents": "The psychiatric resident as an ex-officio community board member. The authors describe a program in which third-year psychiatric residents participate as ex-officio members on the boards of community service agencies. Experience as board members gives residents a broad understanding of issues related to the delivery of community mental health services and encourages them to continue community involvment after their training is completed.", "PMID": 970493}
{"id": "pubmed23n0032_8041", "title": "The use 0f two-way TV in bringing mental health services to the inner city.", "content": "The authors describe a cable TV link between a medical school and a child health station in East Harlem. Nurse associates and community health workers trained through television conferences with a child psychiatrist have the primary responsibility for patient care at the clinic. Patients and their mothers are evaluated by the child psychiatrist in TV consultations at which nurse associates, health workers, medical students, and child psychiatric fellows are present. Patients and mothers respond positively to the system, and a high percentage of the psychiatrist's treatment recommendations are accepted. The authors suggest that such TV links can increase mental health services to underserved inner-city children.", "contents": "The use 0f two-way TV in bringing mental health services to the inner city. The authors describe a cable TV link between a medical school and a child health station in East Harlem. Nurse associates and community health workers trained through television conferences with a child psychiatrist have the primary responsibility for patient care at the clinic. Patients and their mothers are evaluated by the child psychiatrist in TV consultations at which nurse associates, health workers, medical students, and child psychiatric fellows are present. Patients and mothers respond positively to the system, and a high percentage of the psychiatrist's treatment recommendations are accepted. The authors suggest that such TV links can increase mental health services to underserved inner-city children.", "PMID": 970494}
{"id": "pubmed23n0032_8042", "title": "Sixteenth Emil A. Gutheil Memorial Conference. Factors influencing sexual behavior in our future society.", "content": "The paper is concerned with some of the factors that will probably influence sexual behavior at the end of this century and in the beginning of the next. These include changes in family structure, marriage, male-female relationships, effects of large organizations on male-female role playing, mass increase in population, anticipated food shortages, and the effects of forced leisure.", "contents": "Sixteenth Emil A. Gutheil Memorial Conference. Factors influencing sexual behavior in our future society. The paper is concerned with some of the factors that will probably influence sexual behavior at the end of this century and in the beginning of the next. These include changes in family structure, marriage, male-female relationships, effects of large organizations on male-female role playing, mass increase in population, anticipated food shortages, and the effects of forced leisure.", "PMID": 970501}
{"id": "pubmed23n0032_8043", "title": "Beyond sexual freedom: clinical fallout.", "content": "This paper is a critical evaluation of the nature and meaning of various radical changes in sexual customs and behavior and their clinical consequences. The author contends that many of the revolutional changes demonstrate a complete and disastrous disregard of knowledge gained through painstaking psychodynamic and psychoanalytic investigations over the past 75 years.", "contents": "Beyond sexual freedom: clinical fallout. This paper is a critical evaluation of the nature and meaning of various radical changes in sexual customs and behavior and their clinical consequences. The author contends that many of the revolutional changes demonstrate a complete and disastrous disregard of knowledge gained through painstaking psychodynamic and psychoanalytic investigations over the past 75 years.", "PMID": 970502}
{"id": "pubmed23n0032_8044", "title": "Children who are victims of sexual assault and the psychology of offenders.", "content": "Psychiatrists and others have too often discounted reports of sexual attacks upon children and ascribed the incident to fantasy. The author's experience in private psychoanalytic practice and in Philadelphia's rape victim clinics indicates that these assaults occur frequently. If the sexual attack is dealt with improperly or repressed it may cause serious psychologic problems for the victim as an adult.", "contents": "Children who are victims of sexual assault and the psychology of offenders. Psychiatrists and others have too often discounted reports of sexual attacks upon children and ascribed the incident to fantasy. The author's experience in private psychoanalytic practice and in Philadelphia's rape victim clinics indicates that these assaults occur frequently. If the sexual attack is dealt with improperly or repressed it may cause serious psychologic problems for the victim as an adult.", "PMID": 970503}
{"id": "pubmed23n0032_8045", "title": "Adolescent sexuality and societal change.", "content": "Social changes have occurred so rapidly and in such magnitude as to invalidate previously effective coping mechanisms and to reduce the supporting factors in the social milieu. Concomitant with these changes has been an increase in adolescent sexual behavior. Psychoanalytic theory indicates that this behavior will warp or retard psychosexual maturity, but other authorities disagree with this stance. Present evidence does not allow a definitive statement, but indicates the need for more attention to those adolescents who escape psychic damage.", "contents": "Adolescent sexuality and societal change. Social changes have occurred so rapidly and in such magnitude as to invalidate previously effective coping mechanisms and to reduce the supporting factors in the social milieu. Concomitant with these changes has been an increase in adolescent sexual behavior. Psychoanalytic theory indicates that this behavior will warp or retard psychosexual maturity, but other authorities disagree with this stance. Present evidence does not allow a definitive statement, but indicates the need for more attention to those adolescents who escape psychic damage.", "PMID": 970504}
{"id": "pubmed23n0032_8046", "title": "The psychodynamics of male prostitution.", "content": "In a seventeen-month field study thirty-three male were interviewed and tentatively classified into four categories: call-boys, street prostitutes, bar hustlers, and kept boys. These classifications, though flexible, were deemed useful in exploring the homosexual psychodynamics involved. In addition, twenty frequenter of prostitutes (scores) were interviewed and the prostitute-customer relationship was analyzed and discussed.", "contents": "The psychodynamics of male prostitution. In a seventeen-month field study thirty-three male were interviewed and tentatively classified into four categories: call-boys, street prostitutes, bar hustlers, and kept boys. These classifications, though flexible, were deemed useful in exploring the homosexual psychodynamics involved. In addition, twenty frequenter of prostitutes (scores) were interviewed and the prostitute-customer relationship was analyzed and discussed.", "PMID": 970505}
{"id": "pubmed23n0032_8047", "title": "Gossip and psychotherapy.", "content": "Gossip, a phenomenon as old as history itself, is reflected upon in terms of its need-fulfilling functions for persons and social groups. It is seen as one of the sources of knowledge about life that has potentials for wisdom, and its interpersonal and intrapsychic functions are shown to have similarities to many salient functions of psychotherapy.", "contents": "Gossip and psychotherapy. Gossip, a phenomenon as old as history itself, is reflected upon in terms of its need-fulfilling functions for persons and social groups. It is seen as one of the sources of knowledge about life that has potentials for wisdom, and its interpersonal and intrapsychic functions are shown to have similarities to many salient functions of psychotherapy.", "PMID": 970506}
{"id": "pubmed23n0032_8048", "title": "Telepathy in psychoanalysis: an instance.", "content": "A case is presented of a patient telepathically perceiving a stressful event in the analyst's life. Evidence for this is a dream of the patient that embodied a similar content and affect. It is hypothesized that the patient's telepathic ability derived from his self-effacing character trend, which determines an act of perception.", "contents": "Telepathy in psychoanalysis: an instance. A case is presented of a patient telepathically perceiving a stressful event in the analyst's life. Evidence for this is a dream of the patient that embodied a similar content and affect. It is hypothesized that the patient's telepathic ability derived from his self-effacing character trend, which determines an act of perception.", "PMID": 970507}
{"id": "pubmed23n0032_8049", "title": "The stigma of seeing a psychiatrist.", "content": "Mental and physical illnesses are compared and contrasted and reasons for the stigma attached to seeing a psychiatrist are discussed. Psychiatrists appear to share in the responsibility for perpetuating such a stigma. VIP's and others are sometimes prevented from seeking competent help because of such attitudes, which fit into the systems theory model of mental illness. The validity of the medical model is re-emphasized.", "contents": "The stigma of seeing a psychiatrist. Mental and physical illnesses are compared and contrasted and reasons for the stigma attached to seeing a psychiatrist are discussed. Psychiatrists appear to share in the responsibility for perpetuating such a stigma. VIP's and others are sometimes prevented from seeking competent help because of such attitudes, which fit into the systems theory model of mental illness. The validity of the medical model is re-emphasized.", "PMID": 970508}
{"id": "pubmed23n0032_8050", "title": "Psychotherapy of borderline psychotic children.", "content": "This presentation of excerpts from the literature synthesizes many suggestions by various therapists concerning the treatment methods of borderline psychotic children. The importance of providing a stable, auxiliary ego for the child to introject emerges clearly and is clinically demonstrated. Comments on the author's own experiences are included.", "contents": "Psychotherapy of borderline psychotic children. This presentation of excerpts from the literature synthesizes many suggestions by various therapists concerning the treatment methods of borderline psychotic children. The importance of providing a stable, auxiliary ego for the child to introject emerges clearly and is clinically demonstrated. Comments on the author's own experiences are included.", "PMID": 970509}
{"id": "pubmed23n0032_8051", "title": "The mortality of Swedish and U.S. white males: a comparison of experience, 1969-1971.", "content": "The life expectancy of males in the United States is lower than that of males in most of the developed countries and in some of the not-so-developed ones. U.S. females, by contrast, do much better in international ranking. This study compares the mortality of U.S. white males with that of Swedish males who have had the highest reported male life expectancies in the world since the early 1960s. Life expectancy at birth in 1969-71 was 67.9 for U.S. white males compared with 71.9 for Swedish males. Greater U.S. white male mortality is found at all ages from birth through ages 75-79. At the upper ages there is a reversal of the differential with U.S. white males having lower mortality than their Swedish counterparts. The greatest relative differentials between the two male populations is found at ages under 1, ages 20-24, and ages 50-59. At ages under 1 the greater U.S. white male mortality is accounted for mainly by higher death rates from infectious diseases, at ages 20-24 by higher rates from the external causes of death (specifically accidents and homicide), and at ages 50-59 from most of the major organic causes of death.", "contents": "The mortality of Swedish and U.S. white males: a comparison of experience, 1969-1971. The life expectancy of males in the United States is lower than that of males in most of the developed countries and in some of the not-so-developed ones. U.S. females, by contrast, do much better in international ranking. This study compares the mortality of U.S. white males with that of Swedish males who have had the highest reported male life expectancies in the world since the early 1960s. Life expectancy at birth in 1969-71 was 67.9 for U.S. white males compared with 71.9 for Swedish males. Greater U.S. white male mortality is found at all ages from birth through ages 75-79. At the upper ages there is a reversal of the differential with U.S. white males having lower mortality than their Swedish counterparts. The greatest relative differentials between the two male populations is found at ages under 1, ages 20-24, and ages 50-59. At ages under 1 the greater U.S. white male mortality is accounted for mainly by higher death rates from infectious diseases, at ages 20-24 by higher rates from the external causes of death (specifically accidents and homicide), and at ages 50-59 from most of the major organic causes of death.", "PMID": 970513}
{"id": "pubmed23n0032_8052", "title": "An analysis of physician visit data from a complex sample survey.", "content": "A generalization of ordinary least squares methods is used in the analysis of physician visit data from a complex sample survey. The emphasis, in this paper, is on the valid substantive inferences to be drawn from an analysis of this type of data. The procedure is found to be useful in two ways. First, the resultion on a national basis. It is concluded that age is an imp-s of a comparative sampling study are reported. Second, the procedure is used to remove statistically non-significant variation from the data in order to generate fitted or smoothed estimates on which the substantive analyst may focus his attention. These fitted values are then examined for implications to physician service utilization on a national basis. It is concluded that age is an important variable while the effect of sex and race depends on age. Similarly, residence and income are important but the effect of education depends on the level of income.", "contents": "An analysis of physician visit data from a complex sample survey. A generalization of ordinary least squares methods is used in the analysis of physician visit data from a complex sample survey. The emphasis, in this paper, is on the valid substantive inferences to be drawn from an analysis of this type of data. The procedure is found to be useful in two ways. First, the resultion on a national basis. It is concluded that age is an imp-s of a comparative sampling study are reported. Second, the procedure is used to remove statistically non-significant variation from the data in order to generate fitted or smoothed estimates on which the substantive analyst may focus his attention. These fitted values are then examined for implications to physician service utilization on a national basis. It is concluded that age is an important variable while the effect of sex and race depends on age. Similarly, residence and income are important but the effect of education depends on the level of income.", "PMID": 970515}
{"id": "pubmed23n0032_8053", "title": "Quality of care given to first time birth control patients at a free clinic.", "content": "In an empirical study of the quality of care at a free clinic, criteria for optimal care for female first visits for birth control were established and 100 charts were reviewed, 50 in April 1974 and 50 in April 1975 with an interval in between of in-service training accompanied by new medical forms and procedures. An encouraging improvement in record keeping was observed. The authors feel it is important that free clinics concentrate on quality as well as quantity and accessibility of care.", "contents": "Quality of care given to first time birth control patients at a free clinic. In an empirical study of the quality of care at a free clinic, criteria for optimal care for female first visits for birth control were established and 100 charts were reviewed, 50 in April 1974 and 50 in April 1975 with an interval in between of in-service training accompanied by new medical forms and procedures. An encouraging improvement in record keeping was observed. The authors feel it is important that free clinics concentrate on quality as well as quantity and accessibility of care.", "PMID": 970516}
{"id": "pubmed23n0032_8054", "title": "Healing of deep venous thrombosis: venographic findings in a randomized study comparing streptokinase and heparin.", "content": "Sequential ascending venographic studies were used to assess the healing of deep venous thrombosis in 50 patients randomly assigned to streptokinase or heparin therapy. Various degrees of thrombolysis and/or recanalization were demonstrated by venograms performed on the fourth and tenth days of treatment. Late follow-up studies (mean, 7 months after treatment) showed three basic patterns of resolution: (1) return to normal, (2) complete recanalization, and (3) incomplete recanalization and/or collateralization. Loss of valves or their function was associated with recanalization. The character, speed, and outcome of healing reflected the nature and extent of thrombosis, prior thrombotic disease in the extremity, and the type and timing of treatment. Streptokinase was highly effective and preferable to heparin in patients with deep vein thrombosis when therapy was begun within 4 days of onset of symptoms. In later stages of acute or recurrent deep vein thrombosis, the effectiveness of both drugs was significantly reduced.", "contents": "Healing of deep venous thrombosis: venographic findings in a randomized study comparing streptokinase and heparin. Sequential ascending venographic studies were used to assess the healing of deep venous thrombosis in 50 patients randomly assigned to streptokinase or heparin therapy. Various degrees of thrombolysis and/or recanalization were demonstrated by venograms performed on the fourth and tenth days of treatment. Late follow-up studies (mean, 7 months after treatment) showed three basic patterns of resolution: (1) return to normal, (2) complete recanalization, and (3) incomplete recanalization and/or collateralization. Loss of valves or their function was associated with recanalization. The character, speed, and outcome of healing reflected the nature and extent of thrombosis, prior thrombotic disease in the extremity, and the type and timing of treatment. Streptokinase was highly effective and preferable to heparin in patients with deep vein thrombosis when therapy was begun within 4 days of onset of symptoms. In later stages of acute or recurrent deep vein thrombosis, the effectiveness of both drugs was significantly reduced.", "PMID": 970521}
{"id": "pubmed23n0032_8055", "title": "Hemodynamic assessment of obstructive aortoiliac disease.", "content": "Angiographic demonstration of obstructive aortoiliac disease is of paramount importance prior to surgery. Obstructive disease in the femoral popliteal system can only be surgically relieved if inflow is adequate. Severely stenotic lesions may be missed by angiography due to the oblique course of the iliac arteries and inability to obtain right angle views. Translumbar downstream catheterization of the abdominal aorta and puncture of both femoral arteries allows simultaneous pressure recordings. The injection of 30 mg of papaverine into the femoral artery assures maximal vasodilatation mimicking conditions under exercise. A minimal gradient at rest may become obvious following the injection of papaverine, indicating hemodynamically significant disease and warranting surgical correction. The technique has proved to be simple and valuable, and there have been no complications.", "contents": "Hemodynamic assessment of obstructive aortoiliac disease. Angiographic demonstration of obstructive aortoiliac disease is of paramount importance prior to surgery. Obstructive disease in the femoral popliteal system can only be surgically relieved if inflow is adequate. Severely stenotic lesions may be missed by angiography due to the oblique course of the iliac arteries and inability to obtain right angle views. Translumbar downstream catheterization of the abdominal aorta and puncture of both femoral arteries allows simultaneous pressure recordings. The injection of 30 mg of papaverine into the femoral artery assures maximal vasodilatation mimicking conditions under exercise. A minimal gradient at rest may become obvious following the injection of papaverine, indicating hemodynamically significant disease and warranting surgical correction. The technique has proved to be simple and valuable, and there have been no complications.", "PMID": 970522}
{"id": "pubmed23n0032_8056", "title": "Effects of experimental hepatic artery embolization on hepatic function.", "content": "The hepatic artery was embolized with Gelfoam in 12 dogs to evaluate the effect of embolization on hepatic function. Liver function studies, including SGPT, alkaline phosphatase, BSP test, and bilirubin, were done serially over a 6 week period following the embolization. Eleven of the 12 embolized dogs survived the 6 week experimental period. Hepatic artery embolization caused some liver damage; however, recovery occurred by 6 weeks. These results suggest a possible therapeutic role for hepatic artery embolization in patients with massive hepatic bleeding or hemobilia who are not surgical candidates and who would require hepatic lobectomy or dearterialization to control the bleeding.", "contents": "Effects of experimental hepatic artery embolization on hepatic function. The hepatic artery was embolized with Gelfoam in 12 dogs to evaluate the effect of embolization on hepatic function. Liver function studies, including SGPT, alkaline phosphatase, BSP test, and bilirubin, were done serially over a 6 week period following the embolization. Eleven of the 12 embolized dogs survived the 6 week experimental period. Hepatic artery embolization caused some liver damage; however, recovery occurred by 6 weeks. These results suggest a possible therapeutic role for hepatic artery embolization in patients with massive hepatic bleeding or hemobilia who are not surgical candidates and who would require hepatic lobectomy or dearterialization to control the bleeding.", "PMID": 970523}
{"id": "pubmed23n0032_8057", "title": "Traumatic thenar ischemia.", "content": "A case of posttraumatic digital ischemia involving the thenar portion of the hand is presented and the differential diagnosis of hand ischemia is discussed. The importance of obtaining an occupational history for assessment of possible hand trauma is emphasized. Angiography can confirm the diagnosis of posttraumatic arterial injury and will localize thrombosis or aneurysms for possible surgical repair.", "contents": "Traumatic thenar ischemia. A case of posttraumatic digital ischemia involving the thenar portion of the hand is presented and the differential diagnosis of hand ischemia is discussed. The importance of obtaining an occupational history for assessment of possible hand trauma is emphasized. Angiography can confirm the diagnosis of posttraumatic arterial injury and will localize thrombosis or aneurysms for possible surgical repair.", "PMID": 970524}
{"id": "pubmed23n0032_8058", "title": "Sacroiliitis in severe psoriasis.", "content": "In 100 consecutive patients admitted to the hospital for control of severe psoriasis, 25 had radiologic changes characteristic of psoriatic arthritis. Twenty had erosions and mild sclerosis around the sacroiliac joint; in eight of these, asymptomatic sacroiliac changes were the sole manifestation of musculoskeletal disease. Twelve had vertebral syndesmophytes, 11 had terminal interphalangeal joint changes, and 10 had peripheral joint involvement.", "contents": "Sacroiliitis in severe psoriasis. In 100 consecutive patients admitted to the hospital for control of severe psoriasis, 25 had radiologic changes characteristic of psoriatic arthritis. Twenty had erosions and mild sclerosis around the sacroiliac joint; in eight of these, asymptomatic sacroiliac changes were the sole manifestation of musculoskeletal disease. Twelve had vertebral syndesmophytes, 11 had terminal interphalangeal joint changes, and 10 had peripheral joint involvement.", "PMID": 970525}
{"id": "pubmed23n0032_8059", "title": "Posttraumatic adhesive capsulitis of the ankle: arthrographic diagnosis.", "content": "A disabling decrease in range of motion may be a sequelae of ankle trauma. This complication can result from secondary degenerative joint disease, osteochondrial fractures with loose bodies, or posttraumatic adhesive capsulitis. The latter entity is well recognized as a complication of shoulder injuries but is rarely described in other joints. Since adhesive capsulitis produces no specific plain film findings, the confirmation of this diagnosis depends on the usage of a contrast study. It is only recently that ankle arthrography has been utilized to differentiate between the various causes of a painful posttraumatic ankle. Once a diagnosis is established by arthrography, therapeutic efforts can be directed toward relieving the specific abnormality. Three cases of \"frozen\" ankles are presented. Two were the result of intracapsular fractures, while the third had previously sustained a severe injury of the soft tissue. The arthrographic findings included a decrease in the joint capacity, obliteration of the normal anterior and posterior recesses, and extravasation of contrast material along the needle track.", "contents": "Posttraumatic adhesive capsulitis of the ankle: arthrographic diagnosis. A disabling decrease in range of motion may be a sequelae of ankle trauma. This complication can result from secondary degenerative joint disease, osteochondrial fractures with loose bodies, or posttraumatic adhesive capsulitis. The latter entity is well recognized as a complication of shoulder injuries but is rarely described in other joints. Since adhesive capsulitis produces no specific plain film findings, the confirmation of this diagnosis depends on the usage of a contrast study. It is only recently that ankle arthrography has been utilized to differentiate between the various causes of a painful posttraumatic ankle. Once a diagnosis is established by arthrography, therapeutic efforts can be directed toward relieving the specific abnormality. Three cases of \"frozen\" ankles are presented. Two were the result of intracapsular fractures, while the third had previously sustained a severe injury of the soft tissue. The arthrographic findings included a decrease in the joint capacity, obliteration of the normal anterior and posterior recesses, and extravasation of contrast material along the needle track.", "PMID": 970526}
{"id": "pubmed23n0032_8060", "title": "The infrapatellar synovail fold: a cause for confusion in the evaluation of the anterior cruciate ligament.", "content": "The anterior cruciate ligament may be simulated by the normal infrapatellar synovial fold or ligamentum mucosum. With the aid of lateral tomography, the expected course and insertion of the structures can usually be differentiated. This distinction is important, since a torn anterior cruciate ligament can be overlooked and lead to a delay in appropriate therapy.", "contents": "The infrapatellar synovail fold: a cause for confusion in the evaluation of the anterior cruciate ligament. The anterior cruciate ligament may be simulated by the normal infrapatellar synovial fold or ligamentum mucosum. With the aid of lateral tomography, the expected course and insertion of the structures can usually be differentiated. This distinction is important, since a torn anterior cruciate ligament can be overlooked and lead to a delay in appropriate therapy.", "PMID": 970527}
{"id": "pubmed23n0032_8061", "title": "Determination of bone mineral content using CT scanning.", "content": "The usefulness and accuracy of CT scanning in the determination of bone mineral content is studied. The radius in 31 patients of both sexes and varying ages was examined using both the Norland-Cameron bone mineral analyzer and the CT scanner. There was reasonably good correlation (r=.72). Ten cadaver bones were then examined with CT scanning and were sent to the laboratory for calcium determination. These results indicate excellent correlation (r=.97). It is concluded that CT scanning represents the only practical and accurate in vivo method of bone mineral content determination.", "contents": "Determination of bone mineral content using CT scanning. The usefulness and accuracy of CT scanning in the determination of bone mineral content is studied. The radius in 31 patients of both sexes and varying ages was examined using both the Norland-Cameron bone mineral analyzer and the CT scanner. There was reasonably good correlation (r=.72). Ten cadaver bones were then examined with CT scanning and were sent to the laboratory for calcium determination. These results indicate excellent correlation (r=.97). It is concluded that CT scanning represents the only practical and accurate in vivo method of bone mineral content determination.", "PMID": 970528}
{"id": "pubmed23n0032_8062", "title": "Sphenoethmoidal mucocele: radiographic diagnosis.", "content": "Fourteen cases of mucocele of the sphenoid sinus are reviewed to assess differentiating radiographic features. Recognition is usually possible using detailed tomography in the frontal, lateral, and submentovertex projections. Two constant findings were opacification of the sinus (usually bilateral) and expansion and thinning of the sinus walls. Contiguous opacification of the ethmoid sinuses was present in most cases, and pansinusitis was frequent. Distinction from intrasellar lesions is based on the absence of sellar enlargement. Early and correct diagnosis is important to prevent permanent visual loss and to assure appropriate surgical approach.", "contents": "Sphenoethmoidal mucocele: radiographic diagnosis. Fourteen cases of mucocele of the sphenoid sinus are reviewed to assess differentiating radiographic features. Recognition is usually possible using detailed tomography in the frontal, lateral, and submentovertex projections. Two constant findings were opacification of the sinus (usually bilateral) and expansion and thinning of the sinus walls. Contiguous opacification of the ethmoid sinuses was present in most cases, and pansinusitis was frequent. Distinction from intrasellar lesions is based on the absence of sellar enlargement. Early and correct diagnosis is important to prevent permanent visual loss and to assure appropriate surgical approach.", "PMID": 970529}
{"id": "pubmed23n0032_8063", "title": "The radiology of transsphenoidal hypophysectomy: a review of 100 cases.", "content": "This report describing the radiology of transsphenoidal hypophysectomy based on a review of 100 consecutive surgical procedures. Preoperative laminographic evaluation of the sphenoid sinus is an important aid in planning the most direct surgical approach to the pituitary. It is also useful in defining the many anatomic structures that may be injured inadvertently at the time of surgery. Intraoperative radiography or fluoroscopy is helpful in identifying the sella floor and as an aid in preventing loss of orientation within a capacious sinus. Various changes occur within the sphenoid sinus following surgery, some transient and others persistent. The surgical defect is usually difficult to identify on routine roentgenograms but is clearly visible on lateral laminography. A persistent soft tissue mass within the sinus is usually a manifestation of fascia and fat placed as a seal within the sinus.", "contents": "The radiology of transsphenoidal hypophysectomy: a review of 100 cases. This report describing the radiology of transsphenoidal hypophysectomy based on a review of 100 consecutive surgical procedures. Preoperative laminographic evaluation of the sphenoid sinus is an important aid in planning the most direct surgical approach to the pituitary. It is also useful in defining the many anatomic structures that may be injured inadvertently at the time of surgery. Intraoperative radiography or fluoroscopy is helpful in identifying the sella floor and as an aid in preventing loss of orientation within a capacious sinus. Various changes occur within the sphenoid sinus following surgery, some transient and others persistent. The surgical defect is usually difficult to identify on routine roentgenograms but is clearly visible on lateral laminography. A persistent soft tissue mass within the sinus is usually a manifestation of fascia and fat placed as a seal within the sinus.", "PMID": 970530}
{"id": "pubmed23n0032_8064", "title": "Air contrast colon examination in patients with colostomies.", "content": "A readily feasible technique for producing an excellent air contrast examination of the colon in patients with colostomies is described. The technique employes no special equipment and is easily tolerated by patients. Indications for the examination include a symptomatic patient with a negative conventional enema or suspicious single column examination. The surprisingly high incidence of both benign and malignant coexisting colonic neoplasms is reviewed.", "contents": "Air contrast colon examination in patients with colostomies. A readily feasible technique for producing an excellent air contrast examination of the colon in patients with colostomies is described. The technique employes no special equipment and is easily tolerated by patients. Indications for the examination include a symptomatic patient with a negative conventional enema or suspicious single column examination. The surprisingly high incidence of both benign and malignant coexisting colonic neoplasms is reviewed.", "PMID": 970531}
{"id": "pubmed23n0032_8065", "title": "Radiographic manifestations of malignant histiocytosis.", "content": "Twenty-six cases of histologically proven malignant histiocytosis are presented. Patient ages ranged from 20 months to 82 years, and a 10:3 male preponderance was seen. Clinical presentation is similar to that of malignant lymphoma and leukemia; histologic differentiation is required. The frequency of intrathoracic involvement at the time of presentation is similar to that of non-Hodgkin's lymphoma. Retroperitoneal lymph node invasion was readily seen on five of the eight lymphograms performed. Hepatosplenomegaly was a common finding, and three of the patients had evidence of bone involvement during the course of their illness. While there are no radiographic findings specific to malignant histiocytosis, the presence of hepatomegaly should suggest this possibility. The establishment of the correct diagnosis is important to guide choices in chemotherapy.", "contents": "Radiographic manifestations of malignant histiocytosis. Twenty-six cases of histologically proven malignant histiocytosis are presented. Patient ages ranged from 20 months to 82 years, and a 10:3 male preponderance was seen. Clinical presentation is similar to that of malignant lymphoma and leukemia; histologic differentiation is required. The frequency of intrathoracic involvement at the time of presentation is similar to that of non-Hodgkin's lymphoma. Retroperitoneal lymph node invasion was readily seen on five of the eight lymphograms performed. Hepatosplenomegaly was a common finding, and three of the patients had evidence of bone involvement during the course of their illness. While there are no radiographic findings specific to malignant histiocytosis, the presence of hepatomegaly should suggest this possibility. The establishment of the correct diagnosis is important to guide choices in chemotherapy.", "PMID": 970532}
{"id": "pubmed23n0032_8066", "title": "Neonatal diaphragmatic dysfunction.", "content": "Several unusual diaphragmatic problems in the neonate are presented and a new potential complication of the negative pressure respirator is illustrated. The association of bilateral diaphragmatic eventrations with chromosome anomalies is emphasized. In our experience the appearance of the chest on frontal and lateral views is usually so characteristic that no further evaluation is required. The relationship between several neuromuscular disorders and diaphragmatic dysfunction is discussed. A case of congenital myotonic dystrophy with unilateral eventration is illustrated.", "contents": "Neonatal diaphragmatic dysfunction. Several unusual diaphragmatic problems in the neonate are presented and a new potential complication of the negative pressure respirator is illustrated. The association of bilateral diaphragmatic eventrations with chromosome anomalies is emphasized. In our experience the appearance of the chest on frontal and lateral views is usually so characteristic that no further evaluation is required. The relationship between several neuromuscular disorders and diaphragmatic dysfunction is discussed. A case of congenital myotonic dystrophy with unilateral eventration is illustrated.", "PMID": 970533}
{"id": "pubmed23n0032_8067", "title": "Early diagnosis of necrotizing enterocolitis.", "content": "This study demonstrates that the diagnosis of necrotizing enterocolitis can sometimes be made before the full-blown clinical and radiographic findings are manifest. Of 12 infants with films available prior to diagnosis of the disease, nine had early selective dilatation of small bowel and little or no air in the colon. Necrotizing enterocolitis should be foremost in the differential diagnosis when this finding is coupled with signs such as abdominal distention, acidosis, and diarrhea.", "contents": "Early diagnosis of necrotizing enterocolitis. This study demonstrates that the diagnosis of necrotizing enterocolitis can sometimes be made before the full-blown clinical and radiographic findings are manifest. Of 12 infants with films available prior to diagnosis of the disease, nine had early selective dilatation of small bowel and little or no air in the colon. Necrotizing enterocolitis should be foremost in the differential diagnosis when this finding is coupled with signs such as abdominal distention, acidosis, and diarrhea.", "PMID": 970534}
{"id": "pubmed23n0032_8068", "title": "Correlation of renal images on bone scan and intravenous pyelogram.", "content": "The reliability of renal images using the 99mTc-labeled bone-seeking radiopharmaceuticals was evaluated by comparing abnormal renal images on bone scan with IVP and, conversely, abnormal findings on IVP with renal images on bone scan. Several findings on the bone scan renal images were associated with abnormalities on IVP. However, some conditions may not be detected on the bone scan images which are demonstrated on IVP. Results suggest that an abnormality of the renal image on bone scan warrants further investigation while a normal renal image does not exclude renal pathology.", "contents": "Correlation of renal images on bone scan and intravenous pyelogram. The reliability of renal images using the 99mTc-labeled bone-seeking radiopharmaceuticals was evaluated by comparing abnormal renal images on bone scan with IVP and, conversely, abnormal findings on IVP with renal images on bone scan. Several findings on the bone scan renal images were associated with abnormalities on IVP. However, some conditions may not be detected on the bone scan images which are demonstrated on IVP. Results suggest that an abnormality of the renal image on bone scan warrants further investigation while a normal renal image does not exclude renal pathology.", "PMID": 970535}
{"id": "pubmed23n0032_8069", "title": "Angiographic documentation of systemic-portal venous shunting as a cause of a liver scan \"\"hot spot'' in superior vena caval obstruction.", "content": "Four patients with superior vena caval obstruction were evaluated with contrast venography and radiocolloid liver scans to investigate systemic-portal venous shunting as a cause of a liver scan \"hot spot\". In two of the cases a hot spot due to systemic-portal venous shunting was documented by angiography. The venous anatomy and pathophysiology related to hot spot formation on the liver scan is discussed. To develop a liver scan hot spot it appears necessary to have considerable systemic-portal venous flow through anterior parietal and periumbilical venous channels.", "contents": "Angiographic documentation of systemic-portal venous shunting as a cause of a liver scan \"\"hot spot'' in superior vena caval obstruction. Four patients with superior vena caval obstruction were evaluated with contrast venography and radiocolloid liver scans to investigate systemic-portal venous shunting as a cause of a liver scan \"hot spot\". In two of the cases a hot spot due to systemic-portal venous shunting was documented by angiography. The venous anatomy and pathophysiology related to hot spot formation on the liver scan is discussed. To develop a liver scan hot spot it appears necessary to have considerable systemic-portal venous flow through anterior parietal and periumbilical venous channels.", "PMID": 970536}
{"id": "pubmed23n0032_8070", "title": "Carcinoembryonic antigen (CEA) monitoring of radiation therapy for colorectal cancer.", "content": "Serial CEA radioimmunoassays were performed on 16 patients receiving preoperative radiation therapy of rectal cancer or irradiation of recurrent or metastatic colorectal cancer. Radiation therapy of localized colorectal cancer reliably reduced previously elevated circulating CEA titers. Significant decrease of elevated CEA titers with accumulating doses of irradiation may indicate that the bulk of CEA-producing tumor is within the radiation treatment portal and assist in patient management decisions. The decrease of circulating CEA with preoperative radiation therapy was of short duration and may indicate that surgical resection should not be delayed more than 6-8 weeks after irradiation. Because of the high frequency of false positive and false negative results, CEA must be used only in conjunction with other clinical and laboratory parameters.", "contents": "Carcinoembryonic antigen (CEA) monitoring of radiation therapy for colorectal cancer. Serial CEA radioimmunoassays were performed on 16 patients receiving preoperative radiation therapy of rectal cancer or irradiation of recurrent or metastatic colorectal cancer. Radiation therapy of localized colorectal cancer reliably reduced previously elevated circulating CEA titers. Significant decrease of elevated CEA titers with accumulating doses of irradiation may indicate that the bulk of CEA-producing tumor is within the radiation treatment portal and assist in patient management decisions. The decrease of circulating CEA with preoperative radiation therapy was of short duration and may indicate that surgical resection should not be delayed more than 6-8 weeks after irradiation. Because of the high frequency of false positive and false negative results, CEA must be used only in conjunction with other clinical and laboratory parameters.", "PMID": 970537}
{"id": "pubmed23n0032_8071", "title": "Radiation therapy in the management of primary breast cancer.", "content": "Since 1960, six cancer centers in France have used radiotherapy alone (with or without tumorectomy) in the treatment of \"operable\" breast carcinomas. The technique at Hopital Henri Mondor (Creteil) includes a basic irradiation of 5,000 rad to the breast, 4,500 rad to the chest wall, and 4,500 rad to the axillary, supraclavicular, and internal mammary nodal areas. Boost doses of 1,500-2,500 rad to the axillary and internal mammary nodes are given with electrons. A boost dose of 4,000 rad is given to the primary tumor site in the breast by endocureitherapy. Results appear equivalent to those obtained with surgical techniques. Esthetic results are, on the whole, satisfactory. Definitive results will be reported several years hence.", "contents": "Radiation therapy in the management of primary breast cancer. Since 1960, six cancer centers in France have used radiotherapy alone (with or without tumorectomy) in the treatment of \"operable\" breast carcinomas. The technique at Hopital Henri Mondor (Creteil) includes a basic irradiation of 5,000 rad to the breast, 4,500 rad to the chest wall, and 4,500 rad to the axillary, supraclavicular, and internal mammary nodal areas. Boost doses of 1,500-2,500 rad to the axillary and internal mammary nodes are given with electrons. A boost dose of 4,000 rad is given to the primary tumor site in the breast by endocureitherapy. Results appear equivalent to those obtained with surgical techniques. Esthetic results are, on the whole, satisfactory. Definitive results will be reported several years hence.", "PMID": 970538}
{"id": "pubmed23n0032_8072", "title": "Use of auxiliary collimating devices in the treatment for breast cancer with 60Co teletherapy units. I. Dosimetric considerations.", "content": "The dosimetry of tangential field treatment for breast cancer using an auxiliary collimating device on 60Co teletherapy machines is reviewed. The position of the device with respect to the source axis has negligible effect on either the dose rate or the dose distribution. Measurements with thermoluminescent dosimeters in an anatomic phantom verify the accuracy of the method of correction of isodose distributions for sloping skin. Possible systematic errors in dosimetry are identified.", "contents": "Use of auxiliary collimating devices in the treatment for breast cancer with 60Co teletherapy units. I. Dosimetric considerations. The dosimetry of tangential field treatment for breast cancer using an auxiliary collimating device on 60Co teletherapy machines is reviewed. The position of the device with respect to the source axis has negligible effect on either the dose rate or the dose distribution. Measurements with thermoluminescent dosimeters in an anatomic phantom verify the accuracy of the method of correction of isodose distributions for sloping skin. Possible systematic errors in dosimetry are identified.", "PMID": 970539}
{"id": "pubmed23n0032_8073", "title": "Use of axiliary collimating devices in the treatment for breast cancer with 60Co teletherapy units. II. Dose to the skin.", "content": "Special auxiliary collimating devices are often used in radiation therapy for breast cancer in order to treat the chest wall with tangential 60Co fields. Some of these devices are manufactured with a permanently attached Lucite end plate which increases the skin dose due to electron contamination. The relative surface dose was measured on a 60Co irradiator utilizing the collimating device with and without the Lucite plate. A parallel plate extrapolation chamber was used. The surface dose was measured as a function of the distance from the end of the device, the position in the field, and the angle of incidence for typical chest wall fields. These results are used to compare the dose to the skin of the chest wall for several treatment regimes. The data indicate that if the Lucite end plate is permanently attached, the skin dose may be enhanced to the extent that the use of additional bolus may be neither necessary nor desirable.", "contents": "Use of axiliary collimating devices in the treatment for breast cancer with 60Co teletherapy units. II. Dose to the skin. Special auxiliary collimating devices are often used in radiation therapy for breast cancer in order to treat the chest wall with tangential 60Co fields. Some of these devices are manufactured with a permanently attached Lucite end plate which increases the skin dose due to electron contamination. The relative surface dose was measured on a 60Co irradiator utilizing the collimating device with and without the Lucite plate. A parallel plate extrapolation chamber was used. The surface dose was measured as a function of the distance from the end of the device, the position in the field, and the angle of incidence for typical chest wall fields. These results are used to compare the dose to the skin of the chest wall for several treatment regimes. The data indicate that if the Lucite end plate is permanently attached, the skin dose may be enhanced to the extent that the use of additional bolus may be neither necessary nor desirable.", "PMID": 970540}
{"id": "pubmed23n0032_8074", "title": "Simplified method for standardization of roentgenographic image quality using penetration quality measurements.", "content": "A simplified method for standardization of roentgenographic image quality is described. The method employs the concept of penetration quality (PQ) originally established for calibration of x-ray tube potential (kVp). In order to verify that the PQ measurement is useful for standardization of roentgenographic image quality in a clinical situation, a cadaver was selected for patient simulation. It has been shown that PQ may be utilized for roentgenographic technique specification in place of kVp. The validity of this standardization was evaluated subjectively by radiologists.", "contents": "Simplified method for standardization of roentgenographic image quality using penetration quality measurements. A simplified method for standardization of roentgenographic image quality is described. The method employs the concept of penetration quality (PQ) originally established for calibration of x-ray tube potential (kVp). In order to verify that the PQ measurement is useful for standardization of roentgenographic image quality in a clinical situation, a cadaver was selected for patient simulation. It has been shown that PQ may be utilized for roentgenographic technique specification in place of kVp. The validity of this standardization was evaluated subjectively by radiologists.", "PMID": 970541}
{"id": "pubmed23n0032_8075", "title": "Computed tomography in progressive multifocal leukoencephalopathy.", "content": "An area of decreased density found with computed tomography corresponded to confluent demyelinative lesions in the brain of a patient with progressive multifocal leukoencephalopathy. Failure of the CT scan to enhance with contrast material together with the clinical picture may enable clinicians to diagnose this subacutely progressive viral demyelinative brain disorder.", "contents": "Computed tomography in progressive multifocal leukoencephalopathy. An area of decreased density found with computed tomography corresponded to confluent demyelinative lesions in the brain of a patient with progressive multifocal leukoencephalopathy. Failure of the CT scan to enhance with contrast material together with the clinical picture may enable clinicians to diagnose this subacutely progressive viral demyelinative brain disorder.", "PMID": 970542}
{"id": "pubmed23n0032_8076", "title": "Bilateral subclavian steal syndrome.", "content": "Two cases of bilateral subclavian steal syndrome are presented, demonstrating complete occlusion at the origin of both subclavian arteries with distal reconstitution via reversal of blood flow through the vertebrobasilar system. Since unsuspected subclavian artery obstruction may present significant angiographic hazard, careful physical examination including blood pressures in both upper extremities and auscultation of the neck is necessary in order to avoid undesirable complications.", "contents": "Bilateral subclavian steal syndrome. Two cases of bilateral subclavian steal syndrome are presented, demonstrating complete occlusion at the origin of both subclavian arteries with distal reconstitution via reversal of blood flow through the vertebrobasilar system. Since unsuspected subclavian artery obstruction may present significant angiographic hazard, careful physical examination including blood pressures in both upper extremities and auscultation of the neck is necessary in order to avoid undesirable complications.", "PMID": 970543}
{"id": "pubmed23n0032_8077", "title": "Scrotal \"arteriocele\" from iliac artery occlusion.", "content": "A case is described in which a testicular artery, pulsatile and palpable in the scrotum, provided a major collateral for an obstructed iliac artery. The testicular artery was probably spared from occlusion because it originated from a renal artery. Following surgical relief of the iliac obstruction, the testicular artery reverted to normal caliber and was no longer palpable in the scrotum.", "contents": "Scrotal \"arteriocele\" from iliac artery occlusion. A case is described in which a testicular artery, pulsatile and palpable in the scrotum, provided a major collateral for an obstructed iliac artery. The testicular artery was probably spared from occlusion because it originated from a renal artery. Following surgical relief of the iliac obstruction, the testicular artery reverted to normal caliber and was no longer palpable in the scrotum.", "PMID": 970544}
{"id": "pubmed23n0032_8078", "title": "Displaced lateral surface of the liver (Hellmer's sign) secondary to an extraperitoneal fluid collection.", "content": "In 1942, Bellmer (1) first described visualization of a medially displaced lateral liver edge on abdominal radiographs in patients with ascites. In Hellmer's original article and all cases subsequently reported, this appearance was described as being pathognomonic of intraperitoneal fluid. This paper reports the occurrence of Hellmer's sign in a patient with a large extraperitoneal fluid collection extending into the flank. Thus the differential diagnosis associated with a medially displaced lateral liver edge includes pathologic processes in both the intra- and extraperitoneal spaces.", "contents": "Displaced lateral surface of the liver (Hellmer's sign) secondary to an extraperitoneal fluid collection. In 1942, Bellmer (1) first described visualization of a medially displaced lateral liver edge on abdominal radiographs in patients with ascites. In Hellmer's original article and all cases subsequently reported, this appearance was described as being pathognomonic of intraperitoneal fluid. This paper reports the occurrence of Hellmer's sign in a patient with a large extraperitoneal fluid collection extending into the flank. Thus the differential diagnosis associated with a medially displaced lateral liver edge includes pathologic processes in both the intra- and extraperitoneal spaces.", "PMID": 970546}
{"id": "pubmed23n0032_8079", "title": "Genitourinary involvement in chronic granulomatous disease of childhood.", "content": "A second case of symptomatic renal involvement of chronic granulomatous disease of childhood is reported, and all previous cases of documented genitourinary lesions in this disease are reviewed. Although frequent infections of other organ systems are a well recognized part of chronic granulomatous disease, involvement of the urinary tract has been rarely reported. The nature of the few cases described suggests that extensive insidious destruction occurs before urinary tract involvement is clinically suspected.", "contents": "Genitourinary involvement in chronic granulomatous disease of childhood. A second case of symptomatic renal involvement of chronic granulomatous disease of childhood is reported, and all previous cases of documented genitourinary lesions in this disease are reviewed. Although frequent infections of other organ systems are a well recognized part of chronic granulomatous disease, involvement of the urinary tract has been rarely reported. The nature of the few cases described suggests that extensive insidious destruction occurs before urinary tract involvement is clinically suspected.", "PMID": 970547}
{"id": "pubmed23n0032_8080", "title": "Metaphyseal chondroblastoma of bone.", "content": "This report documents the ninth example of a chondroblastoma localized to the metaphysis of a long bone. The tumor appeared as a lytic lesion in the femoral neck of an 18-year-old boy and was associated with a periosteal reaction. When such neoplasms occur in an unusual location with associated periosteal reaction, chondroblastoma should be considered in the differential diagnosis.", "contents": "Metaphyseal chondroblastoma of bone. This report documents the ninth example of a chondroblastoma localized to the metaphysis of a long bone. The tumor appeared as a lytic lesion in the femoral neck of an 18-year-old boy and was associated with a periosteal reaction. When such neoplasms occur in an unusual location with associated periosteal reaction, chondroblastoma should be considered in the differential diagnosis.", "PMID": 970548}
{"id": "pubmed23n0032_8081", "title": "Acupuncture needles: a \"new\" foreign body in the ear.", "content": "With the recent popularity of acupuncture therapy and anesthesia in the United States, patients are having temporary needles placed in the external ear. These matallic radiopaque needles appear on routine skull and mastoid examinations. This article is written to familiarize radiologists with this \"\"new'' iatrogenic foreign body.", "contents": "Acupuncture needles: a \"new\" foreign body in the ear. With the recent popularity of acupuncture therapy and anesthesia in the United States, patients are having temporary needles placed in the external ear. These matallic radiopaque needles appear on routine skull and mastoid examinations. This article is written to familiarize radiologists with this \"\"new'' iatrogenic foreign body.", "PMID": 970549}
{"id": "pubmed23n0032_8082", "title": "The cardiovascular effects of ketamine in man.", "content": "The arterial hypertension of ketamine is prevented by verapamil, a calcium ion antagonist. This indicates that ketamine is a direct myocardial stimulant acting by increasing the availability of calcium across the cell membranes of the myocardial and the Purkinj\u00e9 systems. The positive chronotropic action of ketamine is not reversed by verapamil. Both the chronotropic and the inotropic effects of ketamine are prevented by halothane which acts partly by increased vagal tone and partly by what appears to be a verapamil-like depression of calcium availability. Ketamine blocks the reflex sympathetic response of the peripheral blood vessels to surgical stimuli. The site of the block within the sympathetic reflex arc has not been identified. It is not at the alpha adrenoceptor level. The clinical implications of the findings have been discussed.", "contents": "The cardiovascular effects of ketamine in man. The arterial hypertension of ketamine is prevented by verapamil, a calcium ion antagonist. This indicates that ketamine is a direct myocardial stimulant acting by increasing the availability of calcium across the cell membranes of the myocardial and the Purkinj\u00e9 systems. The positive chronotropic action of ketamine is not reversed by verapamil. Both the chronotropic and the inotropic effects of ketamine are prevented by halothane which acts partly by increased vagal tone and partly by what appears to be a verapamil-like depression of calcium availability. Ketamine blocks the reflex sympathetic response of the peripheral blood vessels to surgical stimuli. The site of the block within the sympathetic reflex arc has not been identified. It is not at the alpha adrenoceptor level. The clinical implications of the findings have been discussed.", "PMID": 970587}
{"id": "pubmed23n0032_8083", "title": "Althesin anaesthesia and a long induction-delivery interval at Caesarean section.", "content": "A case is reported in which obstetric management resulted in 52 minutes elapsing between the induction of anaesthesia and delivery by Caesarean section. The mother was induced and maintained on Althesin and minimal methoxyflurane throughout this period without detriment to the neonate.", "contents": "Althesin anaesthesia and a long induction-delivery interval at Caesarean section. A case is reported in which obstetric management resulted in 52 minutes elapsing between the induction of anaesthesia and delivery by Caesarean section. The mother was induced and maintained on Althesin and minimal methoxyflurane throughout this period without detriment to the neonate.", "PMID": 970588}
{"id": "pubmed23n0032_8084", "title": "Unusual presentation of phaeochromocytoma. Management of anaesthesia and cardiovascular monitoring.", "content": "A case is reported in which a patient with a history of high output left ventricular failure presented for exploratory laparotomy and removal of a highly vascular tumour which was intra-operatively discovered to be a phaeochromocytoma.", "contents": "Unusual presentation of phaeochromocytoma. Management of anaesthesia and cardiovascular monitoring. A case is reported in which a patient with a history of high output left ventricular failure presented for exploratory laparotomy and removal of a highly vascular tumour which was intra-operatively discovered to be a phaeochromocytoma.", "PMID": 970589}
{"id": "pubmed23n0032_8085", "title": "Pulmonary embolism. Prophylaxis diagnosis and treatment.", "content": "The natural history diagnosis and immediate treatment of patients suffering from pulmonary embolism has been discussed. Anaesthetists should use their influence to bring about a high standard of prophylactic care against deep venous thrombosis and consequently of pulmonary embolism. They are likely to be involved in the resuscitation and treatment in intensive care units of those cases who suffer from major symptoms and massive emboli and some of them will rarely be involved in anaesthetising for pulmonary embolectomy aided by cardiopulmonary by-pass and, less rarely, for IVC ligation or plication and venous disobliteration. Anticoagulant drugs appear to limit the mortality of pulmonary embolism to 5%. The mortality of IVC ligation or plication varies in different reports from 2 to 50%; it should therefore be reserved for the special indications which have been discussed. There is also an incidence of recurrent pulmonary embolism after IVC ligation and plication and leg troubles from stasis in about 30% of cases. Streptokinase is usually indicated in the immediate treatment of major pulmonary emboli which cause shock and severe distress with an immediate threat to life. In hospitals having access to cardiopulmonary by-pass, pulmonary embolectomy has a small role to play in major emboli with cardiovascular collapse, if surgery can start within 2 hours and pulmonary angiography is available. Cardiopulmonary by-pass on its own may be life-saving in supporting the circulation while the clot fragments. If cardiac arrest occurs, external cardiac massage should be undertaken as it is sometimes successful and disseminates and fragments the clot in the pulmonary artery.", "contents": "Pulmonary embolism. Prophylaxis diagnosis and treatment. The natural history diagnosis and immediate treatment of patients suffering from pulmonary embolism has been discussed. Anaesthetists should use their influence to bring about a high standard of prophylactic care against deep venous thrombosis and consequently of pulmonary embolism. They are likely to be involved in the resuscitation and treatment in intensive care units of those cases who suffer from major symptoms and massive emboli and some of them will rarely be involved in anaesthetising for pulmonary embolectomy aided by cardiopulmonary by-pass and, less rarely, for IVC ligation or plication and venous disobliteration. Anticoagulant drugs appear to limit the mortality of pulmonary embolism to 5%. The mortality of IVC ligation or plication varies in different reports from 2 to 50%; it should therefore be reserved for the special indications which have been discussed. There is also an incidence of recurrent pulmonary embolism after IVC ligation and plication and leg troubles from stasis in about 30% of cases. Streptokinase is usually indicated in the immediate treatment of major pulmonary emboli which cause shock and severe distress with an immediate threat to life. In hospitals having access to cardiopulmonary by-pass, pulmonary embolectomy has a small role to play in major emboli with cardiovascular collapse, if surgery can start within 2 hours and pulmonary angiography is available. Cardiopulmonary by-pass on its own may be life-saving in supporting the circulation while the clot fragments. If cardiac arrest occurs, external cardiac massage should be undertaken as it is sometimes successful and disseminates and fragments the clot in the pulmonary artery.", "PMID": 970590}
{"id": "pubmed23n0032_8086", "title": "Anaesthesia in patients with phaeochromocytoma.", "content": "The authors' technique of balanced neuroleptanaesthesia with droperidol, fentanyl, nitrous oxide, pancuronium and nitroprusside is outlined. It is suggested that this is the anaesthetic method of choice for the surgical removal of catecholamine secreting tumours since it is associated with good operating conditions and an uneventful post-operative period.", "contents": "Anaesthesia in patients with phaeochromocytoma. The authors' technique of balanced neuroleptanaesthesia with droperidol, fentanyl, nitrous oxide, pancuronium and nitroprusside is outlined. It is suggested that this is the anaesthetic method of choice for the surgical removal of catecholamine secreting tumours since it is associated with good operating conditions and an uneventful post-operative period.", "PMID": 970591}
{"id": "pubmed23n0032_8087", "title": "[Preoperative digitalization. Measurement of digoxin plasma levels (author's transl)].", "content": "In a study of 233 patients from the department of surgery and anesthesiology taking digoxin preparations 64, per cent exhibit digoxin levels in the therapeutic range (0.6--1.5 ng/ml), 19 per cent had subtoxic concentrations ranged from 1.6--2.0 ng/ml and 7 per cent were in the toxic range (greater than 2 ng/ml). In patients treated with digoxin before admission to hospital subtherapeutic levels were most frequent. An average loading dose of digoxin 1 mg or more on one day may result in subtoxic and toxic digoxin levels on the second day, in patients receiving less than 1 mg digoxin daily an increasing frequency of plasma digoxin concentrations of 1.5 ng/ml or higher values was present on the third day. Averaged plasma digoxin concentrations were correlated with daily maintenance dose. There was, however, a wide individual variation in digoxin plasma concentrations. A low incidence of toxic digoxin plasma levels was observed in patients receiving a daily oral maintenance dose of 0.375 mg digoxin (Lanicor). For prophylactic digitalization of patients with normal renal and thyroid function the following schedules or statistical guidlines are proposed: Lanicor (bioavailability 60%): oral loading dose of 0.75 mg over two days, and then daily oral maintenance dose of 0.375 mg; Novodigal (bioavailability 80%): oral loading dose of 0.6 mg over two days and then daily oral maintenance dose of 0.3 mg; Digoxin i.v.: intravenous loading dose of 0.5 (0.4) mg over two days and then 0.25 (0.2) mg daily intravenous maintenance dose. For any patient needing treatment with digitalis glycosides therapy must be individual and dynamic. The reasons for toxic concentrations were frequently attributed to wrong dosage.", "contents": "[Preoperative digitalization. Measurement of digoxin plasma levels (author's transl)]. In a study of 233 patients from the department of surgery and anesthesiology taking digoxin preparations 64, per cent exhibit digoxin levels in the therapeutic range (0.6--1.5 ng/ml), 19 per cent had subtoxic concentrations ranged from 1.6--2.0 ng/ml and 7 per cent were in the toxic range (greater than 2 ng/ml). In patients treated with digoxin before admission to hospital subtherapeutic levels were most frequent. An average loading dose of digoxin 1 mg or more on one day may result in subtoxic and toxic digoxin levels on the second day, in patients receiving less than 1 mg digoxin daily an increasing frequency of plasma digoxin concentrations of 1.5 ng/ml or higher values was present on the third day. Averaged plasma digoxin concentrations were correlated with daily maintenance dose. There was, however, a wide individual variation in digoxin plasma concentrations. A low incidence of toxic digoxin plasma levels was observed in patients receiving a daily oral maintenance dose of 0.375 mg digoxin (Lanicor). For prophylactic digitalization of patients with normal renal and thyroid function the following schedules or statistical guidlines are proposed: Lanicor (bioavailability 60%): oral loading dose of 0.75 mg over two days, and then daily oral maintenance dose of 0.375 mg; Novodigal (bioavailability 80%): oral loading dose of 0.6 mg over two days and then daily oral maintenance dose of 0.3 mg; Digoxin i.v.: intravenous loading dose of 0.5 (0.4) mg over two days and then 0.25 (0.2) mg daily intravenous maintenance dose. For any patient needing treatment with digitalis glycosides therapy must be individual and dynamic. The reasons for toxic concentrations were frequently attributed to wrong dosage.", "PMID": 970596}
{"id": "pubmed23n0032_8088", "title": "[Cardiovascular changes caused by atropine in normo- and hypothermic methoxyflurane anaesthesia (author's transl)].", "content": "The authors examined in 25 patients, of which 15 were normo- and 10 in hypothermic, the cardiovascular effects of 1 mg atropine i.v. In normothermia the heart rate increased significantly from 100 to 110 beats/min after atropine. At the same time the stroke index and stroke work decreased significantly. The mean arterial pressure, the heart index, the left ventricular minute- and stroke work and the total peripheral resistance did not change. In two patients with an arteriovenous fistula and hypervolaemia, the atropine injection caused an increased of heart- and stroke index. Arrhythmias did not occur after atropine. In hypothermia on the other hand atropine was shown to have no effect on heart frequency and all other examined parameters. In one patient in which the P-R interval was shortened, the atropine injection was followed by an total atrio-ventricular block. The authors cannot recommend atropine therapy in cases of hypothermic bradycardia, because of its lack of effect on the heart rate in hypothermia.", "contents": "[Cardiovascular changes caused by atropine in normo- and hypothermic methoxyflurane anaesthesia (author's transl)]. The authors examined in 25 patients, of which 15 were normo- and 10 in hypothermic, the cardiovascular effects of 1 mg atropine i.v. In normothermia the heart rate increased significantly from 100 to 110 beats/min after atropine. At the same time the stroke index and stroke work decreased significantly. The mean arterial pressure, the heart index, the left ventricular minute- and stroke work and the total peripheral resistance did not change. In two patients with an arteriovenous fistula and hypervolaemia, the atropine injection caused an increased of heart- and stroke index. Arrhythmias did not occur after atropine. In hypothermia on the other hand atropine was shown to have no effect on heart frequency and all other examined parameters. In one patient in which the P-R interval was shortened, the atropine injection was followed by an total atrio-ventricular block. The authors cannot recommend atropine therapy in cases of hypothermic bradycardia, because of its lack of effect on the heart rate in hypothermia.", "PMID": 970597}
{"id": "pubmed23n0032_8089", "title": "[Central venous pressure and blood circulation in orthostatic by changes of the total blood volume in postoperative patients (author's transl)].", "content": "In 13 postoperative patients (1st to 9th day) of both sexes, aged 32 to 74, we studied whether an additional volume of 500 ml Haemaccel might demonstrate-as in earlier test series--a correlation between CVP measured in the horizontal position and changes of CVP and Pa measured at atrial level in a partly upright position (tilting angle 45 degrees and 60 degrees of the revolving bed). After volume administration (average: 8.13 ml/kg body weight) and raising of CVP in the horizontal by 3.42 cm H2O corresponding to an E'-value of 0.42 cm H2O/ml/kg body weight, we noticed a significant reduction of the decrease due to orthostasis as well of Pa at heart level as of the \"shock index\"; at the same time, however, the extent of the CVP decrease registered for all patients increased significantly regardless of tilting angle. Therefore, a correlation between the intravasal filling state and the reaction of orthostatic CVP and Pa may be assumed. In conclusion, the explanation for these results and the relation between the venous filling volume and some pathophysiological functions at the right heart are discussed.", "contents": "[Central venous pressure and blood circulation in orthostatic by changes of the total blood volume in postoperative patients (author's transl)]. In 13 postoperative patients (1st to 9th day) of both sexes, aged 32 to 74, we studied whether an additional volume of 500 ml Haemaccel might demonstrate-as in earlier test series--a correlation between CVP measured in the horizontal position and changes of CVP and Pa measured at atrial level in a partly upright position (tilting angle 45 degrees and 60 degrees of the revolving bed). After volume administration (average: 8.13 ml/kg body weight) and raising of CVP in the horizontal by 3.42 cm H2O corresponding to an E'-value of 0.42 cm H2O/ml/kg body weight, we noticed a significant reduction of the decrease due to orthostasis as well of Pa at heart level as of the \"shock index\"; at the same time, however, the extent of the CVP decrease registered for all patients increased significantly regardless of tilting angle. Therefore, a correlation between the intravasal filling state and the reaction of orthostatic CVP and Pa may be assumed. In conclusion, the explanation for these results and the relation between the venous filling volume and some pathophysiological functions at the right heart are discussed.", "PMID": 970598}
{"id": "pubmed23n0032_8090", "title": "[Serum concentrations of pancuronium in anuric patients (author's transl)].", "content": "After a single intravenous dose of 6 mg serum concentrations of pancuronium were measured in 6 anuric patients by means of the bromophenol blue colorimetric method at 1, 5, 45 min and 3 h. After 45 min and 3 h there was a retention of 1.7 and 4 times the control values respectively. The prolongation of pancuronium induced muscular paralysis which has been demonstrated by other authors now can definitely be related to elevated serum concentrations of the active drug. The termination of muscle relaxation in anuric patients is considered merely to be due to redistribution of the drug from the motor end plates to non specific structures. Prolonged neuromuscular blockade may occur in as much as the redistribution mechanism is overloaded by inadequate doses.", "contents": "[Serum concentrations of pancuronium in anuric patients (author's transl)]. After a single intravenous dose of 6 mg serum concentrations of pancuronium were measured in 6 anuric patients by means of the bromophenol blue colorimetric method at 1, 5, 45 min and 3 h. After 45 min and 3 h there was a retention of 1.7 and 4 times the control values respectively. The prolongation of pancuronium induced muscular paralysis which has been demonstrated by other authors now can definitely be related to elevated serum concentrations of the active drug. The termination of muscle relaxation in anuric patients is considered merely to be due to redistribution of the drug from the motor end plates to non specific structures. Prolonged neuromuscular blockade may occur in as much as the redistribution mechanism is overloaded by inadequate doses.", "PMID": 970599}
{"id": "pubmed23n0032_8091", "title": "[Diagnosis of acute acquired defects of coagulation and their therapy (author's transl)].", "content": "Simple coagulation tests, easily performed in every laboratory allowing diagnosis and differential diagnosis of acute acquired defects of coagulation are reported. The therapeutic possibilities and their practice are presented.", "contents": "[Diagnosis of acute acquired defects of coagulation and their therapy (author's transl)]. Simple coagulation tests, easily performed in every laboratory allowing diagnosis and differential diagnosis of acute acquired defects of coagulation are reported. The therapeutic possibilities and their practice are presented.", "PMID": 970600}
{"id": "pubmed23n0032_8092", "title": "Preoperative haemoglobin levels.", "content": "It is a common practice in many anaesthetic centres throughout the world to require a minimum preoperative haemoglobin level of 10g/100 ml of blood or more, but very few references are quoted by writers on this subject. A search of relevant medical literature has been made in an attempt to establish the origin and significance of the preoperative haemoglobin requirements recommended.", "contents": "Preoperative haemoglobin levels. It is a common practice in many anaesthetic centres throughout the world to require a minimum preoperative haemoglobin level of 10g/100 ml of blood or more, but very few references are quoted by writers on this subject. A search of relevant medical literature has been made in an attempt to establish the origin and significance of the preoperative haemoglobin requirements recommended.", "PMID": 970601}
{"id": "pubmed23n0032_8093", "title": "Metabolism of enflurane in man following a second exposure.", "content": "A second administration of one half MAC enflurane for two hours one week following a previous identical exposure caused no increase in urinary inorganic Fluoride excretion (UFV). At this dosage of 1 MAC hour, which is equivalent to use as a supplement, UFV did not sub-anaesthetic doses of enflurane are administered at this time interval there appears to be no increased risk of nephrotoxicity. This study also provides strong support for the concept that drug metabolism is very reproducible within individuals but there is considerable variation between individuals. Since the subjects in this study were all of similar age, and drug free, the results are in keeping with recent twin studies which indicate that individual differences in drug metabolism are largely determined on a genetic basis.", "contents": "Metabolism of enflurane in man following a second exposure. A second administration of one half MAC enflurane for two hours one week following a previous identical exposure caused no increase in urinary inorganic Fluoride excretion (UFV). At this dosage of 1 MAC hour, which is equivalent to use as a supplement, UFV did not sub-anaesthetic doses of enflurane are administered at this time interval there appears to be no increased risk of nephrotoxicity. This study also provides strong support for the concept that drug metabolism is very reproducible within individuals but there is considerable variation between individuals. Since the subjects in this study were all of similar age, and drug free, the results are in keeping with recent twin studies which indicate that individual differences in drug metabolism are largely determined on a genetic basis.", "PMID": 970602}
{"id": "pubmed23n0032_8094", "title": "The hypotensive effect of fresh lympholised plasma.", "content": "The hypotensive effort of fresh, lypholised plasma, both during and after cardiopulmonary bypass is demonstrated. It is postulated that the mediator is bradykinin, or one of its precursors.", "contents": "The hypotensive effect of fresh lympholised plasma. The hypotensive effort of fresh, lypholised plasma, both during and after cardiopulmonary bypass is demonstrated. It is postulated that the mediator is bradykinin, or one of its precursors.", "PMID": 970603}
{"id": "pubmed23n0032_8095", "title": "Post-operative respiratory function: a ventilation-perfusion study.", "content": "Many studies have shown that after uncomplicated abdominal surgery arterial hypoxaemia frequently occurs because of regional underventilation in relation to perfusion. This paper produced evidence that shows a small increase in physiological dead space to tidal volume ratio following general anaesthesia for abdominal surgery. This increase is thought to be due to tachypnoea rather than alteration in pulmonary blood flow.", "contents": "Post-operative respiratory function: a ventilation-perfusion study. Many studies have shown that after uncomplicated abdominal surgery arterial hypoxaemia frequently occurs because of regional underventilation in relation to perfusion. This paper produced evidence that shows a small increase in physiological dead space to tidal volume ratio following general anaesthesia for abdominal surgery. This increase is thought to be due to tachypnoea rather than alteration in pulmonary blood flow.", "PMID": 970604}
{"id": "pubmed23n0032_8096", "title": "Use of an automated register in the administration of an operating theatre suite.", "content": "Slight modification of established recording practices is frequently all that is necessary to enable a computer to be used as a powerful and flexible administrative tool. The organization of an automated data processing system in a hospital operating theatre suite is outlined and discussed.", "contents": "Use of an automated register in the administration of an operating theatre suite. Slight modification of established recording practices is frequently all that is necessary to enable a computer to be used as a powerful and flexible administrative tool. The organization of an automated data processing system in a hospital operating theatre suite is outlined and discussed.", "PMID": 970605}
{"id": "pubmed23n0032_8097", "title": "Thrombus formation on indwelling venous cannulae in sheep : effects of time, size and materials.", "content": "Three experiments were performed to measure thrombus formation on plastic cannulae inserted by cut-down into veins of sheep. On polyethylene tubing, considerable thrombus was present after one day and the amount increased in the subsequent 13 days. When different sized polyethylene tubings were tested, thrombus tended to form in relation to the surface area. Smaller tubings had smaller proportions of their lengths covered with thrombus. There were differences between types of plastics with one batch of polyvinylchloride tubing being virtually non-thrombogenic.", "contents": "Thrombus formation on indwelling venous cannulae in sheep : effects of time, size and materials. Three experiments were performed to measure thrombus formation on plastic cannulae inserted by cut-down into veins of sheep. On polyethylene tubing, considerable thrombus was present after one day and the amount increased in the subsequent 13 days. When different sized polyethylene tubings were tested, thrombus tended to form in relation to the surface area. Smaller tubings had smaller proportions of their lengths covered with thrombus. There were differences between types of plastics with one batch of polyvinylchloride tubing being virtually non-thrombogenic.", "PMID": 970606}
{"id": "pubmed23n0032_8098", "title": "Measurement of thrombus formation on intravascular catheters.", "content": "Relative thrombogenicities of several brands of intravascular catheters were measured in the saphenous and cephalic veins of sheep. Differences in thrombogenicity between brands of up to eleven fold were found. At least one brand contained an ingredient which was locally toxic to sheep and which caused phlebitis in cannulated vessels. The brands tested were made from six types of plastics. It is suggested that differences in manufacturing technique may be more important than the type of plastic in determining the thrombogenicity of catheters.", "contents": "Measurement of thrombus formation on intravascular catheters. Relative thrombogenicities of several brands of intravascular catheters were measured in the saphenous and cephalic veins of sheep. Differences in thrombogenicity between brands of up to eleven fold were found. At least one brand contained an ingredient which was locally toxic to sheep and which caused phlebitis in cannulated vessels. The brands tested were made from six types of plastics. It is suggested that differences in manufacturing technique may be more important than the type of plastic in determining the thrombogenicity of catheters.", "PMID": 970607}
{"id": "pubmed23n0032_8099", "title": "Bicarbonate requirements during hypothermic cardiopulmonary bypass: the relative effects of pulsatile flow.", "content": "An analysis of 541 patients having total body perfusion under hypothermia (30 degrees-33 degrees C) showed that the use of pulsatile as against constant flow perfusion was not associated with a reduction in bicarbonate requirement, though the use of a vasodilator was. The implications of this are discussed.", "contents": "Bicarbonate requirements during hypothermic cardiopulmonary bypass: the relative effects of pulsatile flow. An analysis of 541 patients having total body perfusion under hypothermia (30 degrees-33 degrees C) showed that the use of pulsatile as against constant flow perfusion was not associated with a reduction in bicarbonate requirement, though the use of a vasodilator was. The implications of this are discussed.", "PMID": 970608}
{"id": "pubmed23n0032_8100", "title": "Clinical evaluation of etidocaine in continuous caudal analgesia for pelvic floor repair and post-operative pain relief.", "content": "A randomized double-blind trial compared 1-0% etidocaine and 1-5% lignocaine (both with 1/200,000 adrenaline), for caudal anaesthesia for pelvic floor repair. Etidocaine was highly effective for the surgical procedure, with rapid onset of action, adequate muscle relaxation and longer duration of action. Its use for post-operative analgesia may be hindered by the concomitant immobilization of the legs. The problem of tachyphylaxis with etidocaine needs further investigation.", "contents": "Clinical evaluation of etidocaine in continuous caudal analgesia for pelvic floor repair and post-operative pain relief. A randomized double-blind trial compared 1-0% etidocaine and 1-5% lignocaine (both with 1/200,000 adrenaline), for caudal anaesthesia for pelvic floor repair. Etidocaine was highly effective for the surgical procedure, with rapid onset of action, adequate muscle relaxation and longer duration of action. Its use for post-operative analgesia may be hindered by the concomitant immobilization of the legs. The problem of tachyphylaxis with etidocaine needs further investigation.", "PMID": 970609}
{"id": "pubmed23n0032_8101", "title": "Bronchospasm associated with enflurane exposure--three case reports.", "content": "Three cases of severe bronchospasm occurring in known asthmatics following the administration of enflurance are described. Other commonly known causes of bronchospasm did not seem to be operative. Moderately high concentrations of halothane in the respired gases protected against the bronchospasm whereas a moderately high blood concentration did not. Although the authors have administered enflurane to other known asthmatics without producing bronchospasm, they feel it should be used with caution in such patients.", "contents": "Bronchospasm associated with enflurane exposure--three case reports. Three cases of severe bronchospasm occurring in known asthmatics following the administration of enflurance are described. Other commonly known causes of bronchospasm did not seem to be operative. Moderately high concentrations of halothane in the respired gases protected against the bronchospasm whereas a moderately high blood concentration did not. Although the authors have administered enflurane to other known asthmatics without producing bronchospasm, they feel it should be used with caution in such patients.", "PMID": 970610}
{"id": "pubmed23n0032_8102", "title": "Intercostal block for post operative pain relief.", "content": "Intercostal nerve blocks with 0-5 per cent bupivicaine were used for post operative pain relief in 100 patients having upper abdominal operations. The blocks were very effective in 86 patients and had an average duration of 11 hours. Two asymptomatic pneumothoraces occurred.", "contents": "Intercostal block for post operative pain relief. Intercostal nerve blocks with 0-5 per cent bupivicaine were used for post operative pain relief in 100 patients having upper abdominal operations. The blocks were very effective in 86 patients and had an average duration of 11 hours. Two asymptomatic pneumothoraces occurred.", "PMID": 970611}
{"id": "pubmed23n0032_8103", "title": "Head injury, spasticity, suxamethonium and hyperkalaemia.", "content": "Cardiac arrest due to hyperkalaemia following suxamethonium in a patient with generalized spasticity due to head injury is reported and discussed.", "contents": "Head injury, spasticity, suxamethonium and hyperkalaemia. Cardiac arrest due to hyperkalaemia following suxamethonium in a patient with generalized spasticity due to head injury is reported and discussed.", "PMID": 970612}
{"id": "pubmed23n0032_8104", "title": "A comparative study of intravenous anaesthesia with thiopental versus althesin in outpatient dental practice.", "content": "Clinical effects of two intravenous anaesthetics--thiopental and althesin--were compared in a group of 160 outpatients. Each anaesthetic was used in 80 cases. The administration of these anaesthetics was preceded by an intravenous injection of fentanyl and atropine sulphate. Anaesthesia was carried out with the patient sitting in dental chair. The observations confirmed that this method of anaesthesia provides good conditions for carrying out minor dental operations and is safe for the patient, as evidenced by a negligible number of minor complications. A comparison of the results of clinical observations and gasometric determinations indicates that althesin is superior in outpatient practice to thiopental. This simple and easy technique of anaesthesia without the necessity of using expensive apparatus should be widely introduced in outpatient practice.", "contents": "A comparative study of intravenous anaesthesia with thiopental versus althesin in outpatient dental practice. Clinical effects of two intravenous anaesthetics--thiopental and althesin--were compared in a group of 160 outpatients. Each anaesthetic was used in 80 cases. The administration of these anaesthetics was preceded by an intravenous injection of fentanyl and atropine sulphate. Anaesthesia was carried out with the patient sitting in dental chair. The observations confirmed that this method of anaesthesia provides good conditions for carrying out minor dental operations and is safe for the patient, as evidenced by a negligible number of minor complications. A comparison of the results of clinical observations and gasometric determinations indicates that althesin is superior in outpatient practice to thiopental. This simple and easy technique of anaesthesia without the necessity of using expensive apparatus should be widely introduced in outpatient practice.", "PMID": 970613}
{"id": "pubmed23n0032_8105", "title": "Effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes.", "content": "The purpose of this study was to assess the effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes. The determinations were carried out before anaesthesia, after 1 hour of anaesthesia and 1 hour after awakening. The activity of lysosomal enzymes was assessed on the basis of the activity of marker-enzymes, i.e. acid phosphatase, acid protease and neutral protease. The lysosomal fraction was prepared by Choduker's method and the activity of lysosomal enzymes was calculated for 100 mug of protein. The obtained results showed a statistically not significant rise in the activity of these enzymes in the lysosomes during anaesthesia without a similar rise in the supernatant. The activity of marker enzymes in lysosomes rose to the initial values after the awakening of patients. Such rapid changes in the activity of lysosomal enzymes seem to depend mainly on the mobilization of the reserve pool of granulocytes from other sources than the peripheral blood and, to a lesser extent, on latency and stabilization of lysosomal membranes by anaesthetic agents used in correctly conducted anaesthesia.", "contents": "Effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes. The purpose of this study was to assess the effect of endotracheal anaesthesia on the activity of lysosomal enzymes in peripheral blood granulocytes. The determinations were carried out before anaesthesia, after 1 hour of anaesthesia and 1 hour after awakening. The activity of lysosomal enzymes was assessed on the basis of the activity of marker-enzymes, i.e. acid phosphatase, acid protease and neutral protease. The lysosomal fraction was prepared by Choduker's method and the activity of lysosomal enzymes was calculated for 100 mug of protein. The obtained results showed a statistically not significant rise in the activity of these enzymes in the lysosomes during anaesthesia without a similar rise in the supernatant. The activity of marker enzymes in lysosomes rose to the initial values after the awakening of patients. Such rapid changes in the activity of lysosomal enzymes seem to depend mainly on the mobilization of the reserve pool of granulocytes from other sources than the peripheral blood and, to a lesser extent, on latency and stabilization of lysosomal membranes by anaesthetic agents used in correctly conducted anaesthesia.", "PMID": 970615}
{"id": "pubmed23n0032_8106", "title": "Studies of blood clotting system during dissociative anaesthesia with ketamine.", "content": "The effect of ketamine administered to 20 rabbits in a single dose and in fractionated doses on the blood clotting system was studied. The following blood clotting indices were determined: thrombelastography (TEG), plasma thrombin time, platelet count, euglobulin lysis time. No statistically significant differences were observed in the activity of clotting and fibrinolysis systems in the group of animals receiving a single dose of ketamine and in the group receiving fractionated doses. The obtained results suggest that ketamine as an agent used for dissociative anaesthesia can be administered safely in serious conditions associated with disturbances in the haemostasis system.", "contents": "Studies of blood clotting system during dissociative anaesthesia with ketamine. The effect of ketamine administered to 20 rabbits in a single dose and in fractionated doses on the blood clotting system was studied. The following blood clotting indices were determined: thrombelastography (TEG), plasma thrombin time, platelet count, euglobulin lysis time. No statistically significant differences were observed in the activity of clotting and fibrinolysis systems in the group of animals receiving a single dose of ketamine and in the group receiving fractionated doses. The obtained results suggest that ketamine as an agent used for dissociative anaesthesia can be administered safely in serious conditions associated with disturbances in the haemostasis system.", "PMID": 970617}
{"id": "pubmed23n0032_8107", "title": "The effect of methoxyflurane on certain renal function parameters.", "content": "In 43 patients the effect of low concentrations of methoxyflurane on the renal function was studied. The blood levels of urea, creatinine, uric acid, sodium and potassium, and specific weight of urine was determined. It was found that the anaesthetic technique used and methoxyflurane concentrations in the range of 0.2-0.5 vol% caused no abnormalities in renal function. It seems that methoxyflurane in low concentrations (0.2-0.5 vol%) is the best method of anaesthesia with this agent, with the simultaneous use of a nitrous oxide-oxygen mixture and controlled ventilation.", "contents": "The effect of methoxyflurane on certain renal function parameters. In 43 patients the effect of low concentrations of methoxyflurane on the renal function was studied. The blood levels of urea, creatinine, uric acid, sodium and potassium, and specific weight of urine was determined. It was found that the anaesthetic technique used and methoxyflurane concentrations in the range of 0.2-0.5 vol% caused no abnormalities in renal function. It seems that methoxyflurane in low concentrations (0.2-0.5 vol%) is the best method of anaesthesia with this agent, with the simultaneous use of a nitrous oxide-oxygen mixture and controlled ventilation.", "PMID": 970614}
{"id": "pubmed23n0032_8108", "title": "Plasminogen activators and urokinase inhibitors in the lungs of foetuses and newborns.", "content": "The concentrations of plasminogen activators and urokinase inhibitors were determined in histological sections of 87 foetuses and newborns. It was found that urokinase inhibitors are a constant component of lung tissue in foetuses in the 2nd and 3rd trimesters of pregnancy and in newborns. With progressing maturation of foetuses the concentration of urokinase inhibitors diminishes and, as a rule, the concentration of inhibitors is scores of times higher than that of activators.", "contents": "Plasminogen activators and urokinase inhibitors in the lungs of foetuses and newborns. The concentrations of plasminogen activators and urokinase inhibitors were determined in histological sections of 87 foetuses and newborns. It was found that urokinase inhibitors are a constant component of lung tissue in foetuses in the 2nd and 3rd trimesters of pregnancy and in newborns. With progressing maturation of foetuses the concentration of urokinase inhibitors diminishes and, as a rule, the concentration of inhibitors is scores of times higher than that of activators.", "PMID": 970616}
{"id": "pubmed23n0032_8109", "title": "Anaesthesia for operation of traumatic heart tamponade. A case report.", "content": "The authors report a case of myocardial stab wound followed by shock and symptoms of heart tamponade. Following a discussion on the haemodynamic aspects of this disease entity and description of the case the authors analize the most essential elements of general anaesthesia in such conditions.", "contents": "Anaesthesia for operation of traumatic heart tamponade. A case report. The authors report a case of myocardial stab wound followed by shock and symptoms of heart tamponade. Following a discussion on the haemodynamic aspects of this disease entity and description of the case the authors analize the most essential elements of general anaesthesia in such conditions.", "PMID": 970620}
{"id": "pubmed23n0032_8110", "title": "A simple device designed to protect operating theatres against atmospheric pollution by volatile anaesthetics.", "content": "The authors worked out and applied a new device of positive pressure valve encased in the rebreathing circuit and one-way Ruben's valve, as well as a modification of Rees's system making it possible to conduct excess gases containing anaesthetics outside the operating theatre. These devices and modifications are completely reliable owing to their simplicity and their construction is cheap. They effectively reduce the degree of atmospheric pollution in the operating theatre with anaesthetics used in inhalation anaesthesia. They may be applied to every type of apparatus used for general anaesthesia.", "contents": "A simple device designed to protect operating theatres against atmospheric pollution by volatile anaesthetics. The authors worked out and applied a new device of positive pressure valve encased in the rebreathing circuit and one-way Ruben's valve, as well as a modification of Rees's system making it possible to conduct excess gases containing anaesthetics outside the operating theatre. These devices and modifications are completely reliable owing to their simplicity and their construction is cheap. They effectively reduce the degree of atmospheric pollution in the operating theatre with anaesthetics used in inhalation anaesthesia. They may be applied to every type of apparatus used for general anaesthesia.", "PMID": 970619}
{"id": "pubmed23n0032_8111", "title": "Assessment of acupuncture as a method of analgesia during operation.", "content": "The authors evaluated the method of acupuncture used for analgesia in various types of minor and major surgical operations in patients not previously tested for determination of susceptibility for this method. The report is a preliminary communication. For major operations the patients received premedication and during the operation they received additional local or general analgesics. Out of 13 patients in various age groups 5 evaluated this analgesia as very good and in 3 cases the degree of analgesia obtained was not sufficient to perform the operation. The authors regard that acupuncture has found its main application principally in the treatment of chronic pain, while as a method of analgesia for surgical operations it has many disadvantages and requires further experiences. The authors observed also certain features of acupuncture analgesia not observed in conventional methods of nerve conduction blockade, which may contribute to the explanation of the mechanism of acupuncture.", "contents": "Assessment of acupuncture as a method of analgesia during operation. The authors evaluated the method of acupuncture used for analgesia in various types of minor and major surgical operations in patients not previously tested for determination of susceptibility for this method. The report is a preliminary communication. For major operations the patients received premedication and during the operation they received additional local or general analgesics. Out of 13 patients in various age groups 5 evaluated this analgesia as very good and in 3 cases the degree of analgesia obtained was not sufficient to perform the operation. The authors regard that acupuncture has found its main application principally in the treatment of chronic pain, while as a method of analgesia for surgical operations it has many disadvantages and requires further experiences. The authors observed also certain features of acupuncture analgesia not observed in conventional methods of nerve conduction blockade, which may contribute to the explanation of the mechanism of acupuncture.", "PMID": 970618}
{"id": "pubmed23n0032_8112", "title": "Ketamine used for anaesthesia in children for pneumoencephalography.", "content": "Ketamine was given together with premedication (droperidol + pethidine + atropine) for general anaesthesia in 36 children during pneumoencephalography. Most children (26) had epilepsy. No significant cardiovascular and respiratory disturbances were observed during and after anaesthesia. This proposed method of anaesthesia in children is sufficient for short-lasting diagnostic procedures, particularly for pneumoencephalography.", "contents": "Ketamine used for anaesthesia in children for pneumoencephalography. Ketamine was given together with premedication (droperidol + pethidine + atropine) for general anaesthesia in 36 children during pneumoencephalography. Most children (26) had epilepsy. No significant cardiovascular and respiratory disturbances were observed during and after anaesthesia. This proposed method of anaesthesia in children is sufficient for short-lasting diagnostic procedures, particularly for pneumoencephalography.", "PMID": 970622}
{"id": "pubmed23n0032_8113", "title": "A case of malignant hyperthermia during epidural analgesia.", "content": "Malignant hyperthermia syndrome developed during epidural analgesia in 25-year-old female to be operated on for haemorrhoidal varices. After premedication with diazepam and atropine epidural analgesia was started with lidocaine 300 mg and bupivacaine 50 mg. Signs and symptoms of malignant hyperthermia syndrome appeared 30 min. later, with muscle rigidity, hyperpyrexia 41.5degrees C, and loss of consciousness. Treatment alleviating the syndrome was applied as indicated in this complication. Full recovery was obtained.", "contents": "A case of malignant hyperthermia during epidural analgesia. Malignant hyperthermia syndrome developed during epidural analgesia in 25-year-old female to be operated on for haemorrhoidal varices. After premedication with diazepam and atropine epidural analgesia was started with lidocaine 300 mg and bupivacaine 50 mg. Signs and symptoms of malignant hyperthermia syndrome appeared 30 min. later, with muscle rigidity, hyperpyrexia 41.5degrees C, and loss of consciousness. Treatment alleviating the syndrome was applied as indicated in this complication. Full recovery was obtained.", "PMID": 970624}
{"id": "pubmed23n0032_8114", "title": "The effect of dissociative anaesthesia with ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes of guinea pigs.", "content": "The effect of ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes was studied in guinea pigs. Ketamine was given intramuscularly in one single dose, in divided doses and in long-term administration (repeatedly) for 14 days. The blood for determination was obtained from the heart, the lysosomes were prepared by the Chodirker's method determining the activity of marker enzymes: acid phosphatase, acid protease and neutral protease, in the obtained fractions. No statistically significant differences were found in the activity of lysosomal enzymes in the studied groups. The results obtained point out the usefulness of ketamine in severe conditions associated with significant catabolic changes in the organism.", "contents": "The effect of dissociative anaesthesia with ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes of guinea pigs. The effect of ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes was studied in guinea pigs. Ketamine was given intramuscularly in one single dose, in divided doses and in long-term administration (repeatedly) for 14 days. The blood for determination was obtained from the heart, the lysosomes were prepared by the Chodirker's method determining the activity of marker enzymes: acid phosphatase, acid protease and neutral protease, in the obtained fractions. No statistically significant differences were found in the activity of lysosomal enzymes in the studied groups. The results obtained point out the usefulness of ketamine in severe conditions associated with significant catabolic changes in the organism.", "PMID": 970625}
{"id": "pubmed23n0032_8115", "title": "Spirometric, gasometric and electrocardiographic studies in patients with extreme obesity prepared for surgical treatment.", "content": "Spirometric, gasometric and electrocardiogarphic studies were carried out in 17 patients at rest, who were treated surgically for extreme obesity (overweight from 52 to 175 per cent in relation to the normal weight). The measurements were made before operation. It was found that the mean spirometric values were within the range of acceptable deviations from  the normal value. The residual volume showed a tendency towards decreasing, especially in young patients. The values of dynamic parameters indicated a tendency towards ventilatory changes of a restrictive type in both groups. Minute ventilation at rest increased proportionally to obesity. Decreased values of respiratory equivalent indicated a good economy of respiration in obese persons when at rest. The mean gasometric values in capillary blood at rest were also within the acceptable range of abnormalities. Electrocardiographic tracings taken at rest showed considerable changes in the group of more obese persons.", "contents": "Spirometric, gasometric and electrocardiographic studies in patients with extreme obesity prepared for surgical treatment. Spirometric, gasometric and electrocardiogarphic studies were carried out in 17 patients at rest, who were treated surgically for extreme obesity (overweight from 52 to 175 per cent in relation to the normal weight). The measurements were made before operation. It was found that the mean spirometric values were within the range of acceptable deviations from  the normal value. The residual volume showed a tendency towards decreasing, especially in young patients. The values of dynamic parameters indicated a tendency towards ventilatory changes of a restrictive type in both groups. Minute ventilation at rest increased proportionally to obesity. Decreased values of respiratory equivalent indicated a good economy of respiration in obese persons when at rest. The mean gasometric values in capillary blood at rest were also within the acceptable range of abnormalities. Electrocardiographic tracings taken at rest showed considerable changes in the group of more obese persons.", "PMID": 970621}
{"id": "pubmed23n0032_8116", "title": "The influence of upper respiratory tract receptors stimulation on the respiration and heart action in rats.", "content": "1. Stimulation of the upper respiratory tract with water releases reflex respiratory arrest and very marked bradycardia due to conduction and impulse generation disturbances in the heart accompanied by ventricular hyperexcitability. 2. Asphyxia causes bradycardia as a result of an inhibition of sinus node and the development of vicarious rhythms with excessive ectopic ventricular excitability. The intensity and duration of bradycardia during asphyxia are less than during water stimulation of airways. 3. Urethane anaesthesia did not prevent the development of the above described changes appearing during stimulation of the upper airways while topical analgesia with lidocaine completely prevented their appearance. The observations seem to suggest that for safety, surgical treatment in the upper airways should be done under analgesia of the respiratory mucosa. 4. The mechanism of the development of these changes seems to depend mainly on nervous and humoral factors, although the influence of other factors cannot be excluded. 5. Reflex changes in the action of the heart caused by water stimulation of the upper airways may be one of important components of the complex physiopathological process leading to death in drowning persons.", "contents": "The influence of upper respiratory tract receptors stimulation on the respiration and heart action in rats. 1. Stimulation of the upper respiratory tract with water releases reflex respiratory arrest and very marked bradycardia due to conduction and impulse generation disturbances in the heart accompanied by ventricular hyperexcitability. 2. Asphyxia causes bradycardia as a result of an inhibition of sinus node and the development of vicarious rhythms with excessive ectopic ventricular excitability. The intensity and duration of bradycardia during asphyxia are less than during water stimulation of airways. 3. Urethane anaesthesia did not prevent the development of the above described changes appearing during stimulation of the upper airways while topical analgesia with lidocaine completely prevented their appearance. The observations seem to suggest that for safety, surgical treatment in the upper airways should be done under analgesia of the respiratory mucosa. 4. The mechanism of the development of these changes seems to depend mainly on nervous and humoral factors, although the influence of other factors cannot be excluded. 5. Reflex changes in the action of the heart caused by water stimulation of the upper airways may be one of important components of the complex physiopathological process leading to death in drowning persons.", "PMID": 970626}
{"id": "pubmed23n0032_8117", "title": "Pathomorphology of lungs and heart in experimental prolonged hypoxia.", "content": "The authors carried out morphological examinations of lungs and heart in 20 days with prolonged hypoxia caused by surgical subglottic stenosis of the trachea by 50%. The studies showed that 30 and 60 days after the onset of hypoxia changes such as emphysema and chronic bronchitis developed in the lungs showing an increasing tendency, while in the myocardium microgranular disintegration and fatty infiltration of muscle fibres with simultaneous disappearance of glycogen was seen in the first period-after 30 days - while later, after 60 days fibrosis developed.", "contents": "Pathomorphology of lungs and heart in experimental prolonged hypoxia. The authors carried out morphological examinations of lungs and heart in 20 days with prolonged hypoxia caused by surgical subglottic stenosis of the trachea by 50%. The studies showed that 30 and 60 days after the onset of hypoxia changes such as emphysema and chronic bronchitis developed in the lungs showing an increasing tendency, while in the myocardium microgranular disintegration and fatty infiltration of muscle fibres with simultaneous disappearance of glycogen was seen in the first period-after 30 days - while later, after 60 days fibrosis developed.", "PMID": 970627}
{"id": "pubmed23n0032_8118", "title": "The usefulness of Resusci-Anne manikin in teaching modern methods of resuscitation.", "content": "From experiences gained during the training of personnel the authors evaluate the effectiveness of teaching the principles of modern methods of resuscitation. They concluded that there are no essential differences in the preparation of carrying out resuscitation precedures between laymen and medical personnel. It is necessary to organize refreshing courses periodically. The Recording Resusci-Anne manikin with a device controlling the correctness of resuscitation procedures has been found to be an indispensable training aid in teaching the principles of resuscitation.", "contents": "The usefulness of Resusci-Anne manikin in teaching modern methods of resuscitation. From experiences gained during the training of personnel the authors evaluate the effectiveness of teaching the principles of modern methods of resuscitation. They concluded that there are no essential differences in the preparation of carrying out resuscitation precedures between laymen and medical personnel. It is necessary to organize refreshing courses periodically. The Recording Resusci-Anne manikin with a device controlling the correctness of resuscitation procedures has been found to be an indispensable training aid in teaching the principles of resuscitation.", "PMID": 970623}
{"id": "pubmed23n0032_8119", "title": "A scanning electron microscope study of the interstitial tissue of the canine testis.", "content": "Scanning electron microscopy (SEM) is a potent tool that is especially valuable in interpreting the three-dimensional relationships of cells within tissues. This type of information is obtainable from thin sections in transmission electron microscopy (TEM) only by reconstructions of serial sections. The arrangement of the interstitial cells of the testis in relation to the capillaries and lymphatic channels, in particular, is easier to visualize in SEM than in TEM. Cytoplasmic constituents, as well as cell surface modifications, are demonstrable by this technique. The presence of droplets, presumably lipid droplets, both within and on the Leydig cells and the lymphatic endothelial cells, is quite evident. Other cytoplasmic structures are also apparent. For example, the possible functional significance of \"openings\" that are seen by SEM on the septa that surround lipid droplets is discussed relative to the appearance of the same area as seen in thin sections or in freeze-fracture replicas. SEM should become a very useful method for studying cytological and morphological alterations that occur in testicular tissue that is subjected to physical or chemical manipulation.", "contents": "A scanning electron microscope study of the interstitial tissue of the canine testis. Scanning electron microscopy (SEM) is a potent tool that is especially valuable in interpreting the three-dimensional relationships of cells within tissues. This type of information is obtainable from thin sections in transmission electron microscopy (TEM) only by reconstructions of serial sections. The arrangement of the interstitial cells of the testis in relation to the capillaries and lymphatic channels, in particular, is easier to visualize in SEM than in TEM. Cytoplasmic constituents, as well as cell surface modifications, are demonstrable by this technique. The presence of droplets, presumably lipid droplets, both within and on the Leydig cells and the lymphatic endothelial cells, is quite evident. Other cytoplasmic structures are also apparent. For example, the possible functional significance of \"openings\" that are seen by SEM on the septa that surround lipid droplets is discussed relative to the appearance of the same area as seen in thin sections or in freeze-fracture replicas. SEM should become a very useful method for studying cytological and morphological alterations that occur in testicular tissue that is subjected to physical or chemical manipulation.", "PMID": 970658}
{"id": "pubmed23n0032_8120", "title": "The postnatal development of the junctional complexes of the mouse Sertoli cells as revealed by freeze-fracture.", "content": "Mouse testes of newborn to adult were examined by freeze-fracture. Between the newborn Sertoli cells, gap junctions consisting of aggregations of the intramembranous particles (about 8 nm in diameter) are frequently found. Some of the junctions are about 1 mum in diameter and show particle-free regions in the aggregation. Linear arrangements of a few particles, which appear to be the initial formation of the occluding junctions, are seen in the newborn sertoli cells. The occluding junctions are arranged in a meshwork, in which the gap junctions are situated between the stages of newborn to six days of age. The particles of the occluding junctions are predominantly located on the B face in the center of the groove instead of the A face of the ridge. The occluding junctions do not appear to surround the entire circumference of the Sertoli cell of the 6-day-old mouse. The gap junctions decrease in size. In later stages, many parallel occluding junctions (up to forty in number) are found over one Sertoli cell surface and are distributed circumferentially around the entire cell surface, indicating establishment of the blood-testis barrier. The occluding junctions dominate and the gap junctions diminish in number as development proceeds.", "contents": "The postnatal development of the junctional complexes of the mouse Sertoli cells as revealed by freeze-fracture. Mouse testes of newborn to adult were examined by freeze-fracture. Between the newborn Sertoli cells, gap junctions consisting of aggregations of the intramembranous particles (about 8 nm in diameter) are frequently found. Some of the junctions are about 1 mum in diameter and show particle-free regions in the aggregation. Linear arrangements of a few particles, which appear to be the initial formation of the occluding junctions, are seen in the newborn sertoli cells. The occluding junctions are arranged in a meshwork, in which the gap junctions are situated between the stages of newborn to six days of age. The particles of the occluding junctions are predominantly located on the B face in the center of the groove instead of the A face of the ridge. The occluding junctions do not appear to surround the entire circumference of the Sertoli cell of the 6-day-old mouse. The gap junctions decrease in size. In later stages, many parallel occluding junctions (up to forty in number) are found over one Sertoli cell surface and are distributed circumferentially around the entire cell surface, indicating establishment of the blood-testis barrier. The occluding junctions dominate and the gap junctions diminish in number as development proceeds.", "PMID": 970659}
{"id": "pubmed23n0032_8121", "title": "Parietal eye-pineal morphology in lizards and its physiological implications.", "content": "Pineal complexes in 85 species of lizards examined comprised seven morphological types. Members of the same family do not necessarily have the same pineal complex type. \"Regressive\" parietal eyes were not common except in certain arboreal lizards, primarily from the family Chameleontidae. The parietal eye is often retained in burrowing lizards, presumably because these animals are occasionally exposed to light and the parietal eye is a more suitable photoreceptor for a burrower than are lateral eyes. The pineal of certain lizards possesses a finger-like projection that extends toward the parietal eye. This extension, along with pineal wall convolutions, results in more photoreceptor cells oriented for maximal absorption of light. It is rare to find convolutions and an extension in the same pineal. Cartilage deposits and blood sinuses may modify the intensity and wavelength of light reaching the pineal. These observations suggest that the intracranial pineal of lizards is a more important photoreceptor than was previously realized, a situation that may be a factor in the occasional \"failure\" of parietalectomy experiments.", "contents": "Parietal eye-pineal morphology in lizards and its physiological implications. Pineal complexes in 85 species of lizards examined comprised seven morphological types. Members of the same family do not necessarily have the same pineal complex type. \"Regressive\" parietal eyes were not common except in certain arboreal lizards, primarily from the family Chameleontidae. The parietal eye is often retained in burrowing lizards, presumably because these animals are occasionally exposed to light and the parietal eye is a more suitable photoreceptor for a burrower than are lateral eyes. The pineal of certain lizards possesses a finger-like projection that extends toward the parietal eye. This extension, along with pineal wall convolutions, results in more photoreceptor cells oriented for maximal absorption of light. It is rare to find convolutions and an extension in the same pineal. Cartilage deposits and blood sinuses may modify the intensity and wavelength of light reaching the pineal. These observations suggest that the intracranial pineal of lizards is a more important photoreceptor than was previously realized, a situation that may be a factor in the occasional \"failure\" of parietalectomy experiments.", "PMID": 970660}
{"id": "pubmed23n0032_8122", "title": "Liver hepatocyte mitotic activity after a single injection of phenobarbital in immature male rats.", "content": "In contrast to multiple injections of phenobarbital, a single injection of 50 mg/kg body wt of phenobarbital into immature male rats results, after a transient increase in hepatocyte mitotic activity, in a marked decrease in hepatocyte mitotic activity to below control levels by day 3, followed by a return to control levels by day 5. This unusual pattern of hepatocyte mitotic activity can be called fourth again by a second injection of 50 mg phenobarbital/kg body wt. However, a single injection of 50 mg/kg body wt of phenobarbital into immature male rats results in a pattern of change of liver wet weight, protein, and aminopyrine demethylase activity which is similar to that observed after multiple injections of phenobarbital, except that the changes are smaller in magnitude. Liver wet weight, protein, and aminopyrine demethylase activity increase and reach a peak within two days after phenobarbital injection, and then they return to control levels by five days. The same pattern of change in liver wet weight, protein, and aminopyrine demethylase activity can be elicited again by a second injection of 50 mg phenobarbital/kg body wt.", "contents": "Liver hepatocyte mitotic activity after a single injection of phenobarbital in immature male rats. In contrast to multiple injections of phenobarbital, a single injection of 50 mg/kg body wt of phenobarbital into immature male rats results, after a transient increase in hepatocyte mitotic activity, in a marked decrease in hepatocyte mitotic activity to below control levels by day 3, followed by a return to control levels by day 5. This unusual pattern of hepatocyte mitotic activity can be called fourth again by a second injection of 50 mg phenobarbital/kg body wt. However, a single injection of 50 mg/kg body wt of phenobarbital into immature male rats results in a pattern of change of liver wet weight, protein, and aminopyrine demethylase activity which is similar to that observed after multiple injections of phenobarbital, except that the changes are smaller in magnitude. Liver wet weight, protein, and aminopyrine demethylase activity increase and reach a peak within two days after phenobarbital injection, and then they return to control levels by five days. The same pattern of change in liver wet weight, protein, and aminopyrine demethylase activity can be elicited again by a second injection of 50 mg phenobarbital/kg body wt.", "PMID": 970661}
{"id": "pubmed23n0032_8123", "title": "An IGG1 allotype in cattle.", "content": "This paper presents evidence for an IgG1 allotype detected by a sheep antibovine serum. The character which appears to be inherited in a simple Mendelian way has been named G1a1.", "contents": "An IGG1 allotype in cattle. This paper presents evidence for an IgG1 allotype detected by a sheep antibovine serum. The character which appears to be inherited in a simple Mendelian way has been named G1a1.", "PMID": 970669}
{"id": "pubmed23n0032_8124", "title": "Studies on hemoglobin variants in Korean cattle.", "content": "814 hemoglobin samples from Korean cattle were investigated by starch gel electrophoresis for the detection of hemoglobin variants. A new variant of cattle hemoglobin, called Hb, H, was recognized. It has a slower rate of migration than Hb A. Hemoglobin types from 814 Korean cattle were as follows: 652 of Hb AA type (80.1%), 133 of Hb AB (16.4%), 12 of Hb AC (1.5%), 9 of Hb BB (1.1%), 2 of Hb BC (0.2%), 4 of Hb AH (O.5%), 1 of Hb CH (0.1%), 1 of Hb HH (O.1%). These figures correspond to the frequencies: HbA = 0.893, HbB = 0.093, HbC = 0.009, HbH = 0.004.", "contents": "Studies on hemoglobin variants in Korean cattle. 814 hemoglobin samples from Korean cattle were investigated by starch gel electrophoresis for the detection of hemoglobin variants. A new variant of cattle hemoglobin, called Hb, H, was recognized. It has a slower rate of migration than Hb A. Hemoglobin types from 814 Korean cattle were as follows: 652 of Hb AA type (80.1%), 133 of Hb AB (16.4%), 12 of Hb AC (1.5%), 9 of Hb BB (1.1%), 2 of Hb BC (0.2%), 4 of Hb AH (O.5%), 1 of Hb CH (0.1%), 1 of Hb HH (O.1%). These figures correspond to the frequencies: HbA = 0.893, HbB = 0.093, HbC = 0.009, HbH = 0.004.", "PMID": 970670}
{"id": "pubmed23n0032_8125", "title": "Blood groups and serum protein polymorphisms in the Pitman-Moore and Ohmini strains of miniature pigs.", "content": "The variations of blood groups and biochemical polymorphisms in the populations of the two miniature pig strains reared in Japan were investigated in this report. The variations in eight blood group systems were investigated by using serological techniques, and five serum protein systems by using starch gel electrophoresis. The Pitman-Moore strain showed no variations in K, O, Tf, Cp and Am systems, but showed plymorphisms in A,E,F,G,H,L,Pa and Hpx systems. The Ohmini strain showd no or little variations in E,F,G,Tf,Pa and Cp systems, but showed polymorphisms in A,H,K,L,O,Hpx and Am systems. From the results of investigation of genetic similarities among nine pig populations including various breeds, it was made clear that the two miniature pig strains were very different genetically, and the Pitman-Moore strain was genetically closer to European large pig breeds than to East Asian native pigs, while the Ohmini strain was close to Thai and Philippine natives.", "contents": "Blood groups and serum protein polymorphisms in the Pitman-Moore and Ohmini strains of miniature pigs. The variations of blood groups and biochemical polymorphisms in the populations of the two miniature pig strains reared in Japan were investigated in this report. The variations in eight blood group systems were investigated by using serological techniques, and five serum protein systems by using starch gel electrophoresis. The Pitman-Moore strain showed no variations in K, O, Tf, Cp and Am systems, but showed plymorphisms in A,E,F,G,H,L,Pa and Hpx systems. The Ohmini strain showd no or little variations in E,F,G,Tf,Pa and Cp systems, but showed polymorphisms in A,H,K,L,O,Hpx and Am systems. From the results of investigation of genetic similarities among nine pig populations including various breeds, it was made clear that the two miniature pig strains were very different genetically, and the Pitman-Moore strain was genetically closer to European large pig breeds than to East Asian native pigs, while the Ohmini strain was close to Thai and Philippine natives.", "PMID": 970671}
{"id": "pubmed23n0032_8126", "title": "Electrophoretic variation in intertidal and subtidal organisms in Puget Sound, Washington.", "content": "This paper presents information on the biochemical properties (via starch gel electrophoresis) of 26 marine species (15 fish and 11 invertebrates). Evidence is presented on 48 polymorphisms found in the 26 species. Estimates of the proportion of the genome heterozygous per individual per species were calculated and ranged from 0.000 to 0.176 for the invertebrates and from 0.000 to 0.074 for the fish species. Estimates of the percentage of loci polymorphic per species for invertebrates were 0 to 54%; for the fish species, the range was 0 to 20%.", "contents": "Electrophoretic variation in intertidal and subtidal organisms in Puget Sound, Washington. This paper presents information on the biochemical properties (via starch gel electrophoresis) of 26 marine species (15 fish and 11 invertebrates). Evidence is presented on 48 polymorphisms found in the 26 species. Estimates of the proportion of the genome heterozygous per individual per species were calculated and ranged from 0.000 to 0.176 for the invertebrates and from 0.000 to 0.074 for the fish species. Estimates of the percentage of loci polymorphic per species for invertebrates were 0 to 54%; for the fish species, the range was 0 to 20%.", "PMID": 970672}
{"id": "pubmed23n0032_8127", "title": "Polymorphism of phosphoglucomutase in cattle leucocytes.", "content": "By menas of starch gel electrophoresis the polymorphism of the phospoglucomutase isozymes PGM1 has been investigated in cattle leucocytes. The phenotypess and allele frequencies of PGM1 are demonstrated in a series of 106 complete and 221 incomplete families of \"Deutsche Schwarzbunte\". The segregation of the 327 daughters' phenotypes agree with the formal genetic model of 2 codominant autosomal alleles. The observed heterozygous PGM1 type is more frequent in mating types and progenies as expected under equilibrium.", "contents": "Polymorphism of phosphoglucomutase in cattle leucocytes. By menas of starch gel electrophoresis the polymorphism of the phospoglucomutase isozymes PGM1 has been investigated in cattle leucocytes. The phenotypess and allele frequencies of PGM1 are demonstrated in a series of 106 complete and 221 incomplete families of \"Deutsche Schwarzbunte\". The segregation of the 327 daughters' phenotypes agree with the formal genetic model of 2 codominant autosomal alleles. The observed heterozygous PGM1 type is more frequent in mating types and progenies as expected under equilibrium.", "PMID": 970673}
{"id": "pubmed23n0032_8128", "title": "J blood group active lipoproteins extracted from bovine erythrocytes.", "content": "Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with out finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.", "contents": "J blood group active lipoproteins extracted from bovine erythrocytes. Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with out finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.", "PMID": 970674}
{"id": "pubmed23n0032_8129", "title": "A transferrin variant Tf I in crosses of the wild and domestic pigs.", "content": "In crosses of the wild pig (Sus scrofa attila Thomas) with the domestic pig a transferrin variant, Tf I, was detected, electrophoretic mobility of which was slightly faster than the mobility of the variant Tf A. From the results of starch gel electrophoresis, isolation, neuraminidase treatment, autoradiography, and genetic analysis of several families, it can be concluded that the Tf I variant is genetically controlled by the allele Tf1. Thus the number of alleles in the transferrin system of the pig has increased to six (Tf1, TfA, TfB, TfC, TfD and TfE).", "contents": "A transferrin variant Tf I in crosses of the wild and domestic pigs. In crosses of the wild pig (Sus scrofa attila Thomas) with the domestic pig a transferrin variant, Tf I, was detected, electrophoretic mobility of which was slightly faster than the mobility of the variant Tf A. From the results of starch gel electrophoresis, isolation, neuraminidase treatment, autoradiography, and genetic analysis of several families, it can be concluded that the Tf I variant is genetically controlled by the allele Tf1. Thus the number of alleles in the transferrin system of the pig has increased to six (Tf1, TfA, TfB, TfC, TfD and TfE).", "PMID": 970675}
{"id": "pubmed23n0032_8130", "title": "Environmentally triggered thrombophlebitis.", "content": "Ten randomly selected patients with recurrent non-traumatic thrombophlebitis of unknown etiology were studied using a comprehensive environmental control method. All cleared their phlebitis without medications. Using withdrawal and challenge of incitants, eight of 10 patients had their phlebitis reproduced. The numerous single triggering agents were common-place inhaled and ingested foods and chemicals.", "contents": "Environmentally triggered thrombophlebitis. Ten randomly selected patients with recurrent non-traumatic thrombophlebitis of unknown etiology were studied using a comprehensive environmental control method. All cleared their phlebitis without medications. Using withdrawal and challenge of incitants, eight of 10 patients had their phlebitis reproduced. The numerous single triggering agents were common-place inhaled and ingested foods and chemicals.", "PMID": 970676}
{"id": "pubmed23n0032_8131", "title": "Hypersensitivity as a cause of dermatologic and vaginal moniliasis resistant to topical therapy.", "content": "The finding of multiple respiratory or skin allergies together with a high rate of improvement by desensitization leads the author to conclude that allergy is a predisposing factor for the chronicity or recurrence of vaginal or dermatologic moniliasis. Desensitization with Candida albicans antigen was the mainstay in the treatment of resistant dermatologic or vaginal moniliasis and the sole form of therapy in cases of nail involvement with deep seated lesions out of the reach of topical agents.", "contents": "Hypersensitivity as a cause of dermatologic and vaginal moniliasis resistant to topical therapy. The finding of multiple respiratory or skin allergies together with a high rate of improvement by desensitization leads the author to conclude that allergy is a predisposing factor for the chronicity or recurrence of vaginal or dermatologic moniliasis. Desensitization with Candida albicans antigen was the mainstay in the treatment of resistant dermatologic or vaginal moniliasis and the sole form of therapy in cases of nail involvement with deep seated lesions out of the reach of topical agents.", "PMID": 970677}
{"id": "pubmed23n0032_8132", "title": "High risk factors for childhood asthma.", "content": "Comparing school age asthmatic and non-asthmatic children from strongly allergic families, who contracted atopic dermatitis in early infancy, the following findings in the asthmatic boys emerged: a lower standard of education of their mothers, persistence of the infantile eczema beyond the age of two and several psychological factors relating to the child, the mother and maternal grandparents.", "contents": "High risk factors for childhood asthma. Comparing school age asthmatic and non-asthmatic children from strongly allergic families, who contracted atopic dermatitis in early infancy, the following findings in the asthmatic boys emerged: a lower standard of education of their mothers, persistence of the infantile eczema beyond the age of two and several psychological factors relating to the child, the mother and maternal grandparents.", "PMID": 970678}
{"id": "pubmed23n0032_8133", "title": "Serum immunoglobulins in children with asthma associated with severe respiratory tract infections.", "content": "In a group of 25 selected children with asthma associated with repeated, severe respiratory tract infection, a high incidence of IgA deficiency and elevated IgE levels were found. This may be of importance in the evaluation of such patients.", "contents": "Serum immunoglobulins in children with asthma associated with severe respiratory tract infections. In a group of 25 selected children with asthma associated with repeated, severe respiratory tract infection, a high incidence of IgA deficiency and elevated IgE levels were found. This may be of importance in the evaluation of such patients.", "PMID": 970679}
{"id": "pubmed23n0032_8134", "title": "Studies of atmospheric pollen in Tehran, Iran, 1974-75.", "content": "A Durham sampler was used to study atmospheric pollen in Tehran, 1974-75. More than 18 different pollens were identified and counted. Tree pollens were present from the first week of February to the second week of October. Grass pollens were seen from the first week of May to the middle of November. Weed pollens appeared in the middle of May and were present until the last week of December. Examination of 1,000 patient files indicated that the largest number of patients sought medical help during peaks of pollinations.", "contents": "Studies of atmospheric pollen in Tehran, Iran, 1974-75. A Durham sampler was used to study atmospheric pollen in Tehran, 1974-75. More than 18 different pollens were identified and counted. Tree pollens were present from the first week of February to the second week of October. Grass pollens were seen from the first week of May to the middle of November. Weed pollens appeared in the middle of May and were present until the last week of December. Examination of 1,000 patient files indicated that the largest number of patients sought medical help during peaks of pollinations.", "PMID": 970680}
{"id": "pubmed23n0032_8135", "title": "Light a candle.", "content": "Numerous forces are presently deployed which threaten to produce largescale changes in medical practice and the systems of providing medical care. Some of these changes may be advantageous but others are likely to have deleterious effects on the quality of care, on the physician-patient relationnship and on the physician's freedom to practice as he sees fit. While certain of these pressures cannot be easily diverted, much can be accomplished with a modicum of effort to sway public opinion, the news media, the legislators and the bureaucratic regulation makers. Physicians, both individually and through their societies, must make this effort or suffer the pain of seeing immense decisions made without their leadership or intellectual guidance.", "contents": "Light a candle. Numerous forces are presently deployed which threaten to produce largescale changes in medical practice and the systems of providing medical care. Some of these changes may be advantageous but others are likely to have deleterious effects on the quality of care, on the physician-patient relationnship and on the physician's freedom to practice as he sees fit. While certain of these pressures cannot be easily diverted, much can be accomplished with a modicum of effort to sway public opinion, the news media, the legislators and the bureaucratic regulation makers. Physicians, both individually and through their societies, must make this effort or suffer the pain of seeing immense decisions made without their leadership or intellectual guidance.", "PMID": 970684}
{"id": "pubmed23n0032_8136", "title": "Respiratory mechanics during daily and alternate day corticosteroid therapy in asthma.", "content": "Changes in lung mechanics were measured in asthmatic adults before and after a 10-day course of daily and a three-week course of alternate day adrenocortical steroid therapy. All patients improved after the 10 days of therapy. This response continued during the a.m. measurements on the alternate day regimen with further improvement through the afternoon of the treatment day. In the afternoon of the day off corticosteroid therapy there was a deterioration in flow rates.", "contents": "Respiratory mechanics during daily and alternate day corticosteroid therapy in asthma. Changes in lung mechanics were measured in asthmatic adults before and after a 10-day course of daily and a three-week course of alternate day adrenocortical steroid therapy. All patients improved after the 10 days of therapy. This response continued during the a.m. measurements on the alternate day regimen with further improvement through the afternoon of the treatment day. In the afternoon of the day off corticosteroid therapy there was a deterioration in flow rates.", "PMID": 970685}
{"id": "pubmed23n0032_8137", "title": "Structural variations between pleural and peritoneal mesotheliomas produced in rats by the injection of crocidolite asbestos.", "content": "The author studies the ultrastructure of pleural and peritoneal mesotheliomas caused in the rat by the intra-pleural or intra-peritoneal injection of 25 mg of crocidolite. In the peritoneum the tumour is made up chiefly of \"connective\" type tissue with rare \"epithelial\" cells. In the pleura, the \"epithelial\" cells predominate, constituting vast vegetating \"papillomatous\" zones or \"adenomatous\" territories. The author recalls Willis' concept, and feels that mesothelial tumours in the rat, caused by asbestos, represent one aspect of the family of mesenchymatous tumours, with their ability to behave differently according to their site.", "contents": "Structural variations between pleural and peritoneal mesotheliomas produced in rats by the injection of crocidolite asbestos. The author studies the ultrastructure of pleural and peritoneal mesotheliomas caused in the rat by the intra-pleural or intra-peritoneal injection of 25 mg of crocidolite. In the peritoneum the tumour is made up chiefly of \"connective\" type tissue with rare \"epithelial\" cells. In the pleura, the \"epithelial\" cells predominate, constituting vast vegetating \"papillomatous\" zones or \"adenomatous\" territories. The author recalls Willis' concept, and feels that mesothelial tumours in the rat, caused by asbestos, represent one aspect of the family of mesenchymatous tumours, with their ability to behave differently according to their site.", "PMID": 970686}
{"id": "pubmed23n0032_8138", "title": "Tumours in experimental animals following exposure to asbestos dust.", "content": "The author summarises his research into experimental asbestos-related tumours. He notes in particular the inequality in carcinogenic power of different types of asbestos and the variations in the types of malignant tumour seen (carcinomas or mesotheliomas), according to the variety of asbestos used.", "contents": "Tumours in experimental animals following exposure to asbestos dust. The author summarises his research into experimental asbestos-related tumours. He notes in particular the inequality in carcinogenic power of different types of asbestos and the variations in the types of malignant tumour seen (carcinomas or mesotheliomas), according to the variety of asbestos used.", "PMID": 970687}
{"id": "pubmed23n0032_8139", "title": "[Activity of blood somatomedin during childhood in normal subjects and in delayed growth].", "content": "A hundred sera from children, were used to evaluate normal levels of somatomedin activity and pathological levels. The results showed that the level of this activity in the blood, rises gradually during infancy, to reach normal adult reference level by postulate, equal to 1. Although the values are widely dispersed in each sample, the general phenomenon of their increase in infancy remains significant. The patients with somatotropin deficiency, have very low somatomedin values which become normal under the influence of injection of human growth hormone. A very unusual case is that of Laron's dwarfism with very high values of HGH in the plasma, and very low levels of somatomedin and, clinical and laboratory indifference to injections of exogenous HGH. The prospects of progress in the techniques of study of somatomedin will be of great service in the near future.", "contents": "[Activity of blood somatomedin during childhood in normal subjects and in delayed growth]. A hundred sera from children, were used to evaluate normal levels of somatomedin activity and pathological levels. The results showed that the level of this activity in the blood, rises gradually during infancy, to reach normal adult reference level by postulate, equal to 1. Although the values are widely dispersed in each sample, the general phenomenon of their increase in infancy remains significant. The patients with somatotropin deficiency, have very low somatomedin values which become normal under the influence of injection of human growth hormone. A very unusual case is that of Laron's dwarfism with very high values of HGH in the plasma, and very low levels of somatomedin and, clinical and laboratory indifference to injections of exogenous HGH. The prospects of progress in the techniques of study of somatomedin will be of great service in the near future.", "PMID": 970694}
{"id": "pubmed23n0032_8140", "title": "[Anatomopathologic study of 54 cases of diffuse pleural mesothelioma found in the harbor regions of Nantes, Saint-Nazaire and Lorient].", "content": "The study concerned the macroscopic and microscopic examination of 54 cases of diffuse malignant mesothelioma. Four points were felt to be of interest and worthy of emphasis: --the unilateral nature of the lesions, constant in this series; --the absence of involvement of other serous surfaces; --the tumour-like appearance of the disorder in 2/3 of cases and the appearance of a simple pachy-pleuritis in 1/3; --from a histological standpoint, despite the morphological flexibility of the classically accepted mesothelial cells, the homogenicity of our group, in which tumour morphology was much more epithelial than histiocytic or connective.", "contents": "[Anatomopathologic study of 54 cases of diffuse pleural mesothelioma found in the harbor regions of Nantes, Saint-Nazaire and Lorient]. The study concerned the macroscopic and microscopic examination of 54 cases of diffuse malignant mesothelioma. Four points were felt to be of interest and worthy of emphasis: --the unilateral nature of the lesions, constant in this series; --the absence of involvement of other serous surfaces; --the tumour-like appearance of the disorder in 2/3 of cases and the appearance of a simple pachy-pleuritis in 1/3; --from a histological standpoint, despite the morphological flexibility of the classically accepted mesothelial cells, the homogenicity of our group, in which tumour morphology was much more epithelial than histiocytic or connective.", "PMID": 970689}
{"id": "pubmed23n0032_8141", "title": "[Study of the specificity of action of cholesterol oxygen oxidoreductase on various sterols and related substances].", "content": "The authors studied the action of oxygen cholesterol oxidoreductase (E.C. 1.1.3.6) extracted from Nocardia erythropolis on different sterols following simultaneously:--the disappearance of the substrate by chromatography on thin layers of silic acid, the formation of hydrogen peroxide by R\u00f6schlau's reaction, changes in the absorption spectrum between 220 and 320 nm. The results obtained show that sterol oxidase presents a broad spectrum of activity. It catalyses the transformation into ketone of the secondary alcohol group at the 3 beta position of sterols which possess in the C17 position a lateral chain of at least two carbon atoms. In the other hand, the reaction does not take place if there exists in the C5 position, an H in the cis position or if two hydrogen atoms linked to carbon 4 are substituted. Finally, only oxidation products derived from sterols possess a double bond situated in the 4-5 or 5-6 position presenting an absorption band between 240 and 250 nm.", "contents": "[Study of the specificity of action of cholesterol oxygen oxidoreductase on various sterols and related substances]. The authors studied the action of oxygen cholesterol oxidoreductase (E.C. 1.1.3.6) extracted from Nocardia erythropolis on different sterols following simultaneously:--the disappearance of the substrate by chromatography on thin layers of silic acid, the formation of hydrogen peroxide by R\u00f6schlau's reaction, changes in the absorption spectrum between 220 and 320 nm. The results obtained show that sterol oxidase presents a broad spectrum of activity. It catalyses the transformation into ketone of the secondary alcohol group at the 3 beta position of sterols which possess in the C17 position a lateral chain of at least two carbon atoms. In the other hand, the reaction does not take place if there exists in the C5 position, an H in the cis position or if two hydrogen atoms linked to carbon 4 are substituted. Finally, only oxidation products derived from sterols possess a double bond situated in the 4-5 or 5-6 position presenting an absorption band between 240 and 250 nm.", "PMID": 970695}
{"id": "pubmed23n0032_8142", "title": "[The oncogenic effect of fibrous dust. Animal experiments and their relationship with human carcinogenesis].", "content": "The results after intraperitoneal injection of fibrous and granular dusts in rats suggest, that the fibrous shape of asbestos is the cause of its cancerogenicity. Fibrous dusts other than asbestos (for example glass fibers) may induce tumours, when their diameters are like that of asbestos and the fibres are invariable in tissue. It is supposed, that a fibre with a diameter less than 1 mum and a length greater than 3 mum has a cancerogenic effect. The interpretation of the experimental results considering the cancerogenesis in humans is discussed, especially concerning the effect of fibrous glass.", "contents": "[The oncogenic effect of fibrous dust. Animal experiments and their relationship with human carcinogenesis]. The results after intraperitoneal injection of fibrous and granular dusts in rats suggest, that the fibrous shape of asbestos is the cause of its cancerogenicity. Fibrous dusts other than asbestos (for example glass fibers) may induce tumours, when their diameters are like that of asbestos and the fibres are invariable in tissue. It is supposed, that a fibre with a diameter less than 1 mum and a length greater than 3 mum has a cancerogenic effect. The interpretation of the experimental results considering the cancerogenesis in humans is discussed, especially concerning the effect of fibrous glass.", "PMID": 970688}
{"id": "pubmed23n0032_8143", "title": "[Micromethod of diazepam determination in the blood by gas-liquid chromatography. Application in neonatal pharmacology].", "content": "We propose a micromethod of diazepam determination on 0,1 ml of whole blood. The extraction is made with n-heptane for the isolation of diazepam in presence of principal metabolites. For the usual concentrations, the precision is about 3 per cent; at the detection limite, 0,05 mug/ml, it is about 20 per cent. With this method we studied the pharmacokinetics of diazepam in neonate with convulsive diseases in comparison with electroencephalogram.", "contents": "[Micromethod of diazepam determination in the blood by gas-liquid chromatography. Application in neonatal pharmacology]. We propose a micromethod of diazepam determination on 0,1 ml of whole blood. The extraction is made with n-heptane for the isolation of diazepam in presence of principal metabolites. For the usual concentrations, the precision is about 3 per cent; at the detection limite, 0,05 mug/ml, it is about 20 per cent. With this method we studied the pharmacokinetics of diazepam in neonate with convulsive diseases in comparison with electroencephalogram.", "PMID": 970696}
{"id": "pubmed23n0032_8144", "title": "[Simple and totally automatic continuous flow technic for the determination of triglycerides in the blood].", "content": "The author describes a simple and entirely automatic technique for the estimation of triglycerides in human serum which, in acetic medium, are extracted by a mixture of nonane-isopropanol and saponified. The glycerol is oxidised into formaldehyde which is estimated by fluorimetry after Hantzsch' reaction. Successive stages are discussed together with the precision of the method. The results are in agreement with those obtained by Kessler and Lederer's method.", "contents": "[Simple and totally automatic continuous flow technic for the determination of triglycerides in the blood]. The author describes a simple and entirely automatic technique for the estimation of triglycerides in human serum which, in acetic medium, are extracted by a mixture of nonane-isopropanol and saponified. The glycerol is oxidised into formaldehyde which is estimated by fluorimetry after Hantzsch' reaction. Successive stages are discussed together with the precision of the method. The results are in agreement with those obtained by Kessler and Lederer's method.", "PMID": 970697}
{"id": "pubmed23n0032_8145", "title": "[Asbestosis with sarcoid-like pulmonary lesions. A propos of one anatomoclinical case report].", "content": "A report of a clinico-pathological case of asbestosis with special histological lesions. The latter had a granulomatous appearance, progressing to fibrosis with Schaumann bodies organised around bodies with a ferruginous covering, apparently due to asbestosis. The histogenesis of the lesions is discussed, together with the need to take a precise professional history. The usefulness of certain simple methods of analysis is mentioned.", "contents": "[Asbestosis with sarcoid-like pulmonary lesions. A propos of one anatomoclinical case report]. A report of a clinico-pathological case of asbestosis with special histological lesions. The latter had a granulomatous appearance, progressing to fibrosis with Schaumann bodies organised around bodies with a ferruginous covering, apparently due to asbestosis. The histogenesis of the lesions is discussed, together with the need to take a precise professional history. The usefulness of certain simple methods of analysis is mentioned.", "PMID": 970691}
{"id": "pubmed23n0032_8146", "title": "[5 minutes incubation for the radioimmunologic determination of somatomammotrophic chorionic hormone].", "content": "A radioimmunoassay procedure for human chorionic somatomammotrophin in indiluted plasma has been developed in which the incubation lasts only five minutes rather than one half hour to four days in the previously described methods. The values obtained had excellent correlation (p less than or equal to 0,001) with those obtained by a two-hours technique. The intra-assay and inter-assay coefficients of variation are respectively 7 percent and 11 percent. Twenty women at term showed human chorionic somatomammotrophin values of 7.5 +/- 2.5 mug/ml plasma (mean +/- SD).", "contents": "[5 minutes incubation for the radioimmunologic determination of somatomammotrophic chorionic hormone]. A radioimmunoassay procedure for human chorionic somatomammotrophin in indiluted plasma has been developed in which the incubation lasts only five minutes rather than one half hour to four days in the previously described methods. The values obtained had excellent correlation (p less than or equal to 0,001) with those obtained by a two-hours technique. The intra-assay and inter-assay coefficients of variation are respectively 7 percent and 11 percent. Twenty women at term showed human chorionic somatomammotrophin values of 7.5 +/- 2.5 mug/ml plasma (mean +/- SD).", "PMID": 970698}
{"id": "pubmed23n0032_8147", "title": "[Automatic microdetermination of serotinin in capillary blood].", "content": "The authors describe an automatic fluorimetric micromethod based on the reaction of condensation of serotonin with orthophthaldialdehyde forming a fluorescent compound. This precise and sensitive method permits estimation of serotonin on microsamples. The results obtained on capillary blood are identical to those obtained on venous blood.", "contents": "[Automatic microdetermination of serotinin in capillary blood]. The authors describe an automatic fluorimetric micromethod based on the reaction of condensation of serotonin with orthophthaldialdehyde forming a fluorescent compound. This precise and sensitive method permits estimation of serotonin on microsamples. The results obtained on capillary blood are identical to those obtained on venous blood.", "PMID": 970699}
{"id": "pubmed23n0032_8148", "title": "[Enzymatic activity assays in the hepatic cell by mass fragmentography associated with gas-liquid chromatography].", "content": "The most generalized methods in enzymology are based on the quantitative assay of compounds, substrate or coenzyme, by spectrophotometry without any separation. Such a method is ruled out if the colorimetric reaction is not specific of the compound. In liver enzymology, aside the classical metabolic pathways, such assays are difficult to apply, especially when several metabolic steps are investigated. It is therefore necessary to use separative methods to isolate the metabolized substrate(s). For instance, the reductive catabolism of corticosterone leads to fourteen isomers (two dihydrocompounds, four tetrahydrocompounds and eight hexahydrocompounds) in which their respective productions are sex and age-linked. A position isomer of corticosterone, the 18-hydroxy-11-deoxy-corticosterone, follows the same reductive route. In adrenals some reduced metabolites arise from these two steroid hormones and are age dependent. When such metabolites are amenable to volatilization for gas chromatography, the interfacing of the gas chromatograph to the mass spectrometer allows to identify each compound introduced in the spectrometer. Among the ions produced by fragmentation of a compound or of a family of compounds, several specific fragments can be selected to be monitored along the chromatographic run leading to mass peaks which are quantitatively proportional to the amount of compounds, as far as other foreign molecules do not contribute fo fragment productions. These methods called mass fragmentography or multiple ion detection, or selected ion monitoring, allow with the help of all the resources of gas chromatography such the derivatization of studied molecules with heavy isotope labeled reagents to use the same unlabeled derivatized molecules as carriers and internal standards at once. This method allows to quantitate at the level of the picomolecule or less. Examples will be given with the study of the metabolism hormone steroids and xenobiotic compounds by the liver and adrenals in the animal and by isolated liver and adrenal cell cultures.", "contents": "[Enzymatic activity assays in the hepatic cell by mass fragmentography associated with gas-liquid chromatography]. The most generalized methods in enzymology are based on the quantitative assay of compounds, substrate or coenzyme, by spectrophotometry without any separation. Such a method is ruled out if the colorimetric reaction is not specific of the compound. In liver enzymology, aside the classical metabolic pathways, such assays are difficult to apply, especially when several metabolic steps are investigated. It is therefore necessary to use separative methods to isolate the metabolized substrate(s). For instance, the reductive catabolism of corticosterone leads to fourteen isomers (two dihydrocompounds, four tetrahydrocompounds and eight hexahydrocompounds) in which their respective productions are sex and age-linked. A position isomer of corticosterone, the 18-hydroxy-11-deoxy-corticosterone, follows the same reductive route. In adrenals some reduced metabolites arise from these two steroid hormones and are age dependent. When such metabolites are amenable to volatilization for gas chromatography, the interfacing of the gas chromatograph to the mass spectrometer allows to identify each compound introduced in the spectrometer. Among the ions produced by fragmentation of a compound or of a family of compounds, several specific fragments can be selected to be monitored along the chromatographic run leading to mass peaks which are quantitatively proportional to the amount of compounds, as far as other foreign molecules do not contribute fo fragment productions. These methods called mass fragmentography or multiple ion detection, or selected ion monitoring, allow with the help of all the resources of gas chromatography such the derivatization of studied molecules with heavy isotope labeled reagents to use the same unlabeled derivatized molecules as carriers and internal standards at once. This method allows to quantitate at the level of the picomolecule or less. Examples will be given with the study of the metabolism hormone steroids and xenobiotic compounds by the liver and adrenals in the animal and by isolated liver and adrenal cell cultures.", "PMID": 970700}
{"id": "pubmed23n0032_8149", "title": "[Peripheral airway obstruction in the course of occupational exposure to asbestos].", "content": "Typically, the functional abnormalities in individuals exposed to asbestos dust take the form of a pulmonary fibrosis. Recently, several authors have stressed the early existence of small airways obstruction. In the present study, by measurement of closing volume, we have confirmed an obstructive factor in a certain number of exposed subjects, even if they were non-smokers, i.e. had no other apparent reason for small airways disease.", "contents": "[Peripheral airway obstruction in the course of occupational exposure to asbestos]. Typically, the functional abnormalities in individuals exposed to asbestos dust take the form of a pulmonary fibrosis. Recently, several authors have stressed the early existence of small airways obstruction. In the present study, by measurement of closing volume, we have confirmed an obstructive factor in a certain number of exposed subjects, even if they were non-smokers, i.e. had no other apparent reason for small airways disease.", "PMID": 970690}
{"id": "pubmed23n0032_8150", "title": "[Spectrofluorometric determination of furosemide in biological fluids. Application to pharmaco- and biokinetic studies].", "content": "The present report describes spectrofluorimetric methods for evaluation of furosemide (a sulfamide diuretic derived from anthranilic acid) in plasma or urine. The availability of these methods is discussed. These methods can be used for pharmacokinetic and pharmacological studies and sometimes resolution of therapeutic problems.", "contents": "[Spectrofluorometric determination of furosemide in biological fluids. Application to pharmaco- and biokinetic studies]. The present report describes spectrofluorimetric methods for evaluation of furosemide (a sulfamide diuretic derived from anthranilic acid) in plasma or urine. The availability of these methods is discussed. These methods can be used for pharmacokinetic and pharmacological studies and sometimes resolution of therapeutic problems.", "PMID": 970702}
{"id": "pubmed23n0032_8151", "title": "[Bacteroides fragilis. Its isolation and antibiotic sensitivity].", "content": "The authors chose the problem of the isolation of bacteroides fragilis in pathological samples to present a method of identification of strict anaerobic gram negative bacteria, suitable for use in a routine bacteriological laboratory. A selection of 20 strains led them to study the mean inhibitory concentration of these germs with regard to antibiotics. From this study, two groups were obtained: one of low activity, the other of greater activity in vitro, in which one may note certain tetracyclines, clindamycin and metronidazole.", "contents": "[Bacteroides fragilis. Its isolation and antibiotic sensitivity]. The authors chose the problem of the isolation of bacteroides fragilis in pathological samples to present a method of identification of strict anaerobic gram negative bacteria, suitable for use in a routine bacteriological laboratory. A selection of 20 strains led them to study the mean inhibitory concentration of these germs with regard to antibiotics. From this study, two groups were obtained: one of low activity, the other of greater activity in vitro, in which one may note certain tetracyclines, clindamycin and metronidazole.", "PMID": 970703}
{"id": "pubmed23n0032_8152", "title": "[Topographical variations of pulmonary and pleural asbestos fiber concentrations in variously exposed subjects].", "content": "The authors study three groups of subjects with different exposures to asbestos dust. The lung content in asbestos fibres is measured by optical and electron microscopy, the latter technique revealing preferential concentration of asbestos fibres in the peripheral zones of the lung, in particular in individuals subject to a moderate degree of professional exposure.", "contents": "[Topographical variations of pulmonary and pleural asbestos fiber concentrations in variously exposed subjects]. The authors study three groups of subjects with different exposures to asbestos dust. The lung content in asbestos fibres is measured by optical and electron microscopy, the latter technique revealing preferential concentration of asbestos fibres in the peripheral zones of the lung, in particular in individuals subject to a moderate degree of professional exposure.", "PMID": 970692}
{"id": "pubmed23n0032_8153", "title": "[Urinary excretion of 17-ketosteroids studied by gas chromatography in the healthy adult: distribution of values].", "content": "The level of different urinary 17-ketosteroids were assayed using a method requiring an enzymatic hydrolysis and a solvolysis, a separation by gas-chromatography on OV 225 (cyanopropylsilicone) and a detection by flame ionisation. The method has been discuted. Statistical study of the results obtained in 89 healthy and ambulatory subjects (divided in 4 groups according to sex and age) has shown a log-normal distribution of urinary excretions.", "contents": "[Urinary excretion of 17-ketosteroids studied by gas chromatography in the healthy adult: distribution of values]. The level of different urinary 17-ketosteroids were assayed using a method requiring an enzymatic hydrolysis and a solvolysis, a separation by gas-chromatography on OV 225 (cyanopropylsilicone) and a detection by flame ionisation. The method has been discuted. Statistical study of the results obtained in 89 healthy and ambulatory subjects (divided in 4 groups according to sex and age) has shown a log-normal distribution of urinary excretions.", "PMID": 970704}
{"id": "pubmed23n0032_8154", "title": "Respiratory function and symptoms in urban office workers in relation to oxidant air pollution exposure.", "content": "Similar populations of male and female office workers in San Francisco, which has little air pollution, and in Los Angeles, which experiences frequent photochemical smog episodes, were surveyed in an attempt to document excess respiratory symptoms and dysfunction in Los Angeles relatable to air pollution. Most results of forced expiratory tests, single-breath N2 tests, and questionnaire interviews did not differ significantly between cities. Los Angeles women reported nonpersistent cough and phlegm more often than did San Francisco women. Smokers in both cities showed increased functional abnormalities. These results suggested that Los Angeles oxidant exposure is far less significant than smoking as a risk factor in development of chronic respiratory disease in sedentary indoor workers in good general health. Oxidant exposure has not been ruled out as a significant risk to more heavily exposed on more highly susceptible persons.", "contents": "Respiratory function and symptoms in urban office workers in relation to oxidant air pollution exposure. Similar populations of male and female office workers in San Francisco, which has little air pollution, and in Los Angeles, which experiences frequent photochemical smog episodes, were surveyed in an attempt to document excess respiratory symptoms and dysfunction in Los Angeles relatable to air pollution. Most results of forced expiratory tests, single-breath N2 tests, and questionnaire interviews did not differ significantly between cities. Los Angeles women reported nonpersistent cough and phlegm more often than did San Francisco women. Smokers in both cities showed increased functional abnormalities. These results suggested that Los Angeles oxidant exposure is far less significant than smoking as a risk factor in development of chronic respiratory disease in sedentary indoor workers in good general health. Oxidant exposure has not been ruled out as a significant risk to more heavily exposed on more highly susceptible persons.", "PMID": 970728}
{"id": "pubmed23n0032_8155", "title": "Household aggregation of pulmonary function and chronic bronchitis.", "content": "Persons from 148 randomly selected households in an urban community were screened in their homes using a modified British Medical Research Council respiratory disease questionnaire and a portable spirometer. Analysis showed a significant tendency for chronic bronchitis to aggregate within households. Significant aggregation was observed for 1-sec forced expiratory volume, when measured as the per cent of the predicted value or as a score calculated from the data. The 1-sec forced expiratory volume was significantly correlated between siblings, but less clearly so between spouses. Correlation of 1-sec forced expiratory volume between mother and child appeared to be confounded by maternal smoking habits, an effect most notable between mothers and male offspring. The 1-sec forced expiratory volume of fathers was significantly correlated with that of their children, especially female children, an effect that appeared to be independent of smoking habits.", "contents": "Household aggregation of pulmonary function and chronic bronchitis. Persons from 148 randomly selected households in an urban community were screened in their homes using a modified British Medical Research Council respiratory disease questionnaire and a portable spirometer. Analysis showed a significant tendency for chronic bronchitis to aggregate within households. Significant aggregation was observed for 1-sec forced expiratory volume, when measured as the per cent of the predicted value or as a score calculated from the data. The 1-sec forced expiratory volume was significantly correlated between siblings, but less clearly so between spouses. Correlation of 1-sec forced expiratory volume between mother and child appeared to be confounded by maternal smoking habits, an effect most notable between mothers and male offspring. The 1-sec forced expiratory volume of fathers was significantly correlated with that of their children, especially female children, an effect that appeared to be independent of smoking habits.", "PMID": 970729}
{"id": "pubmed23n0032_8156", "title": "Inhaled and oral salbutamol in exercise-induced asthma.", "content": "Twenty-one asthmatic patients participated in a single blind, crossover study comparing the effects of aerosol and oral salbutamol (a beta-adrenergic bronchodilator), on exercise-induced bronchospasm. By both routes of administration, salbutamol (albuterol) produced significant bronchodilatation at rest, but only the aerosol effectively prevented post-exercise bronchospasm in most patients. Because exercise-induced bronchospasm is a common problem in children and adults with asthma, these observations are relevant to the drug therapy of these patients. The existence of separate mechanisms for bronchodilatation and the prevention of exercise-induced asthma is postulated. It is possible that differing tissue concentrations of salbutamol are responsible for the observed differences between the 2 routes of administration.", "contents": "Inhaled and oral salbutamol in exercise-induced asthma. Twenty-one asthmatic patients participated in a single blind, crossover study comparing the effects of aerosol and oral salbutamol (a beta-adrenergic bronchodilator), on exercise-induced bronchospasm. By both routes of administration, salbutamol (albuterol) produced significant bronchodilatation at rest, but only the aerosol effectively prevented post-exercise bronchospasm in most patients. Because exercise-induced bronchospasm is a common problem in children and adults with asthma, these observations are relevant to the drug therapy of these patients. The existence of separate mechanisms for bronchodilatation and the prevention of exercise-induced asthma is postulated. It is possible that differing tissue concentrations of salbutamol are responsible for the observed differences between the 2 routes of administration.", "PMID": 970730}
{"id": "pubmed23n0032_8157", "title": "Frequency dependence of total respiratory resistance in early airway disease.", "content": "Patients who develop frequency dependence of lung compliance will theoretically have frequency dependence of pulmonary resistance. We investigated the ability of the simpler, noninvasive measurement of frequency dependence of total respiratory resistance to identify subjects with frequency dependence of compliance. Ten healthy nonsmokers, 14 asymptomatic smokers, and 6 patients with obstructive airway disease were studied. Frequency dependence of total respiratory resistance was determined by the superimposed oscillating airflow technique at 3 to 9 cycles per sec, and frequency dependence of lung compliance was determined by measurements at 10 to 80 breaths per min. Spirometry, airway resistance, closing volume, and closing capacity were also measured. Frequency dependence of lung compliance and total respiratory resistance were closely correlated (P less than 0.001, r = 0.82), but closing volume, closing capacity, spirometry, and airway resistance could not be used to identify subjects with abnormal frequency dependence of lung compliance. Measurements of frequency dependence of total respiratory resistance and lung compliance, total respiratory resistance at 3 cycles per sec, and closing volume minus expiratory reserve volume were able to distinguish significantly between the smokers and the nonsmokers, but spirometry, closing volume, closing capacity, and airway resistance could not. These data indicate that in asymptomatic smokers and subjects with obstructive airway disease, frequency dependence of lung compliance can be predicted from measurements of frequency dependence of total respiratory resistance. These two tests appear to have equivalent sensitivity and selectivity in detecting the uneven time constants in the airways of asymptomatic smokers.", "contents": "Frequency dependence of total respiratory resistance in early airway disease. Patients who develop frequency dependence of lung compliance will theoretically have frequency dependence of pulmonary resistance. We investigated the ability of the simpler, noninvasive measurement of frequency dependence of total respiratory resistance to identify subjects with frequency dependence of compliance. Ten healthy nonsmokers, 14 asymptomatic smokers, and 6 patients with obstructive airway disease were studied. Frequency dependence of total respiratory resistance was determined by the superimposed oscillating airflow technique at 3 to 9 cycles per sec, and frequency dependence of lung compliance was determined by measurements at 10 to 80 breaths per min. Spirometry, airway resistance, closing volume, and closing capacity were also measured. Frequency dependence of lung compliance and total respiratory resistance were closely correlated (P less than 0.001, r = 0.82), but closing volume, closing capacity, spirometry, and airway resistance could not be used to identify subjects with abnormal frequency dependence of lung compliance. Measurements of frequency dependence of total respiratory resistance and lung compliance, total respiratory resistance at 3 cycles per sec, and closing volume minus expiratory reserve volume were able to distinguish significantly between the smokers and the nonsmokers, but spirometry, closing volume, closing capacity, and airway resistance could not. These data indicate that in asymptomatic smokers and subjects with obstructive airway disease, frequency dependence of lung compliance can be predicted from measurements of frequency dependence of total respiratory resistance. These two tests appear to have equivalent sensitivity and selectivity in detecting the uneven time constants in the airways of asymptomatic smokers.", "PMID": 970731}
{"id": "pubmed23n0032_8158", "title": "The response of asthmatic subjects to isoproterenol inhaled at differing lung volumes.", "content": "Asthmatics are usually instructed to use pressurized bronchodilator aerosols by delivering a bolus of drug at the beginning of a full inspiration. Because airways are better dilated near total lung capacity, the delivery of the drug near the end of a full breath might allow better penetration of particles into the lung and greater bronchodilatation. To test this hypothesis, 13 asthmatic subjects inhaled 400 mug of isoproterenol at 20 per cent (low) and at 80 per cent (high) vital capacity. The studies were done on 2 separate days when the severity of asthma was the same. Forced vital capacity, 1-sec forced expiratory volume, specific airway conductance and maximal flow at 50 per cent of viral capacity were measured at frequent intervals after drug administration. Ten min after drug delivery, there was a significantly greater (P less than 0.05) improvement in 1-sec forced expiratory volume after the drug was inhaled at the high lung volume compared to the response after delivery at the low lung volume. The differences in forced vital capacity, specific conductance, and maximal flow at 50 per cent of vital capacity were not significant. We concluded that inhaling a bronchodilator drug at the end of a full inspiration causes relatively greater bronchodilatation than inhaling the same dose at the beginning of inspiration.", "contents": "The response of asthmatic subjects to isoproterenol inhaled at differing lung volumes. Asthmatics are usually instructed to use pressurized bronchodilator aerosols by delivering a bolus of drug at the beginning of a full inspiration. Because airways are better dilated near total lung capacity, the delivery of the drug near the end of a full breath might allow better penetration of particles into the lung and greater bronchodilatation. To test this hypothesis, 13 asthmatic subjects inhaled 400 mug of isoproterenol at 20 per cent (low) and at 80 per cent (high) vital capacity. The studies were done on 2 separate days when the severity of asthma was the same. Forced vital capacity, 1-sec forced expiratory volume, specific airway conductance and maximal flow at 50 per cent of viral capacity were measured at frequent intervals after drug administration. Ten min after drug delivery, there was a significantly greater (P less than 0.05) improvement in 1-sec forced expiratory volume after the drug was inhaled at the high lung volume compared to the response after delivery at the low lung volume. The differences in forced vital capacity, specific conductance, and maximal flow at 50 per cent of vital capacity were not significant. We concluded that inhaling a bronchodilator drug at the end of a full inspiration causes relatively greater bronchodilatation than inhaling the same dose at the beginning of inspiration.", "PMID": 970732}
{"id": "pubmed23n0032_8159", "title": "Inhaled atropine sulfate: dose response characteristics.", "content": "Inhaled atropine sulfate is a bronchodilator in man, but its efficacy and optimal dosage are still unknown. In the current study, the dose-response characteristics of this agent were examined in 20 children with chronic perennial asthma, using maximal expiratory flow-volume curves. Inhaled drug was delivered in incremental doses of 0.005 to 0.1 mg per kg. A peak action of atropine on the maximal flow after exhalation of 50 per cent of the forced vital capacity was seen 1 hour after inhalation; significant bronchodilatation was observed for as long as 300 min, depending on the dose. A plateau was observed in the dose-response curve in the dose range, 0.05 to 0.1 mg per kg. Changes in the maximal expiratory flow after exhalation of 75 per cent of the forced vital capacity paralleled those in the maximal expiratory flow after exhalation of 50 per cent of the forced vital capacity. Specific conductance significantly increased, and the ratio of residual volume to total lung capacity significantly decreased in 10 patients examined. In no case could the observed increases in flows be explained by the patient's breathing at a higher lung volume. Considerable intersubject variation was found in the effects of a given dose of atropine. This may be explained by variations in baseline flows. It was concluded that atropine sulfate by inhalation is an effective bronchodilator in children with severe asthma and that reported variations in its efficacy may be related to inadequate dosage.", "contents": "Inhaled atropine sulfate: dose response characteristics. Inhaled atropine sulfate is a bronchodilator in man, but its efficacy and optimal dosage are still unknown. In the current study, the dose-response characteristics of this agent were examined in 20 children with chronic perennial asthma, using maximal expiratory flow-volume curves. Inhaled drug was delivered in incremental doses of 0.005 to 0.1 mg per kg. A peak action of atropine on the maximal flow after exhalation of 50 per cent of the forced vital capacity was seen 1 hour after inhalation; significant bronchodilatation was observed for as long as 300 min, depending on the dose. A plateau was observed in the dose-response curve in the dose range, 0.05 to 0.1 mg per kg. Changes in the maximal expiratory flow after exhalation of 75 per cent of the forced vital capacity paralleled those in the maximal expiratory flow after exhalation of 50 per cent of the forced vital capacity. Specific conductance significantly increased, and the ratio of residual volume to total lung capacity significantly decreased in 10 patients examined. In no case could the observed increases in flows be explained by the patient's breathing at a higher lung volume. Considerable intersubject variation was found in the effects of a given dose of atropine. This may be explained by variations in baseline flows. It was concluded that atropine sulfate by inhalation is an effective bronchodilator in children with severe asthma and that reported variations in its efficacy may be related to inadequate dosage.", "PMID": 970733}
{"id": "pubmed23n0032_8160", "title": "Carbon dioxide response lines in young adults: the limits of the normal response.", "content": "Carbon dioxide responsiveness was determined in 126 normal, young adults. The mean +/- SE slope of the line relating PCO2 to minute volume of ventilation was 2.60 +/- 0.107 liter per min-mm Hg. Although a wide range of responses was noted, 80 percent of the subjects had a value for the slope between 1.5 and 5.0 liter per min - mm Hg, with 16.6 per cent having values lying below and 4 per cent above this range. Women were significantly less responsive than men. Low, but significant, correlations were found between the slope and vital capacity, and between the slope and maximal breathing capacity, but not between the slope and height, weight, age, or smoking habits. Additional findings included an increase in the slope with prolonged rebreathing, possibly due to a peripheral chemoreceptor effect; absence of metabolic acidosis during 4 min of rebreathing; excellent short-term reproducibility of results in 111 subjects; and good longer-term reproducibility in most of 10 subjects retested 9 to 24 months later.", "contents": "Carbon dioxide response lines in young adults: the limits of the normal response. Carbon dioxide responsiveness was determined in 126 normal, young adults. The mean +/- SE slope of the line relating PCO2 to minute volume of ventilation was 2.60 +/- 0.107 liter per min-mm Hg. Although a wide range of responses was noted, 80 percent of the subjects had a value for the slope between 1.5 and 5.0 liter per min - mm Hg, with 16.6 per cent having values lying below and 4 per cent above this range. Women were significantly less responsive than men. Low, but significant, correlations were found between the slope and vital capacity, and between the slope and maximal breathing capacity, but not between the slope and height, weight, age, or smoking habits. Additional findings included an increase in the slope with prolonged rebreathing, possibly due to a peripheral chemoreceptor effect; absence of metabolic acidosis during 4 min of rebreathing; excellent short-term reproducibility of results in 111 subjects; and good longer-term reproducibility in most of 10 subjects retested 9 to 24 months later.", "PMID": 970734}
{"id": "pubmed23n0032_8161", "title": "Respiratory abnormalities and ventilatory capacity in a Papua New Guinea Island community.", "content": "On Karkar Island, off the coast of Papua New Guinea, 87 per cent of the 1,026 resident adults of 3 villages were surveyed for respiratory abnormalities. The prevalence of abnormalities suggestive of chronic lung disease was similar for each sex and increased with age. After 35 years of age, the combined rates were: adventitious breath sounds, 29 per cent; positive loose cough sign, 33 per cent; chronic cough, 11 per cent; and shortness of breath on exertion, 12 per cent. All of these abnormalities were associated with an obstructive ventilatory defect. Smoking began in late adolescence and was established in more than 90 per cent of both sexes by 25 years of age. Most persons smoked a home-grown variety of Nicotiana tabacum. The nonsmoking group was too small to allow adequate comparison; but among smokers, inhalation was related to both respiratory abnormalities and reduced lung function. The prevalence of respiratory abnormalities appeared to be at least as high as in some developed western societies, despite the fact that smoking habits resembled those of cigar smokers elsewhere, and that atmospheric and occupational exposures were absent. Among persons more than 35 years of age, 4 per cent had a history of pulmonary tuberculosis, and 5 per cent showed evidence of localized lung disease in a 70-mm chest radiograph. The predominant condition resembled the chronic nonspecific lung disease of developed societies. Unless this population is unusually susceptible to the effect of cigar-type smoking, other etiologic factors must be considered. These might include repeated acute chest infections, the indirect effects of pulmonary tuberculosis, larval migrations of intestinal parasites, or impaired host response as a result of poor nutrition. A wide spectrum of severity coexisted with a uniform environment and smoking pattern, which suggests that individual susceptibility is important.", "contents": "Respiratory abnormalities and ventilatory capacity in a Papua New Guinea Island community. On Karkar Island, off the coast of Papua New Guinea, 87 per cent of the 1,026 resident adults of 3 villages were surveyed for respiratory abnormalities. The prevalence of abnormalities suggestive of chronic lung disease was similar for each sex and increased with age. After 35 years of age, the combined rates were: adventitious breath sounds, 29 per cent; positive loose cough sign, 33 per cent; chronic cough, 11 per cent; and shortness of breath on exertion, 12 per cent. All of these abnormalities were associated with an obstructive ventilatory defect. Smoking began in late adolescence and was established in more than 90 per cent of both sexes by 25 years of age. Most persons smoked a home-grown variety of Nicotiana tabacum. The nonsmoking group was too small to allow adequate comparison; but among smokers, inhalation was related to both respiratory abnormalities and reduced lung function. The prevalence of respiratory abnormalities appeared to be at least as high as in some developed western societies, despite the fact that smoking habits resembled those of cigar smokers elsewhere, and that atmospheric and occupational exposures were absent. Among persons more than 35 years of age, 4 per cent had a history of pulmonary tuberculosis, and 5 per cent showed evidence of localized lung disease in a 70-mm chest radiograph. The predominant condition resembled the chronic nonspecific lung disease of developed societies. Unless this population is unusually susceptible to the effect of cigar-type smoking, other etiologic factors must be considered. These might include repeated acute chest infections, the indirect effects of pulmonary tuberculosis, larval migrations of intestinal parasites, or impaired host response as a result of poor nutrition. A wide spectrum of severity coexisted with a uniform environment and smoking pattern, which suggests that individual susceptibility is important.", "PMID": 970735}
{"id": "pubmed23n0032_8162", "title": "Bird breeder's hypersensitivity pneumonitis: progress studies of lung function after cessation of exposure to the provoking antigen.", "content": "Lung function and immunologic studies have been performed in 9 patients with bird breeder's hypersensitivity pneumonitis at the time of diagnosis and at intervals from 8 to 30 months after they ceased exposure to the provoking antigen. All 9 patients had evidence of small airway obstruction at the time of diagnosis. Complete recovery of lung function occurred in 4 patients. The other 5 all had evidence of interstitial damage; 3 of them had progressive increase in the degree of airway obstruction, and one had progressive loss of elastic recoil. The patients were nonsmokers, and these progressive changes could not be explained by occult exposure to antigen because the tests of immunologic sensitivity to relevant avian antigens became negative. Neither the nature or degree of lung function abnormality nor the form of clinical presentation was related to the development of residual damage. The period of exposure to antigen after symptoms developed and the age of the patient appeared to be the most important factors determining recovery of lung function.", "contents": "Bird breeder's hypersensitivity pneumonitis: progress studies of lung function after cessation of exposure to the provoking antigen. Lung function and immunologic studies have been performed in 9 patients with bird breeder's hypersensitivity pneumonitis at the time of diagnosis and at intervals from 8 to 30 months after they ceased exposure to the provoking antigen. All 9 patients had evidence of small airway obstruction at the time of diagnosis. Complete recovery of lung function occurred in 4 patients. The other 5 all had evidence of interstitial damage; 3 of them had progressive increase in the degree of airway obstruction, and one had progressive loss of elastic recoil. The patients were nonsmokers, and these progressive changes could not be explained by occult exposure to antigen because the tests of immunologic sensitivity to relevant avian antigens became negative. Neither the nature or degree of lung function abnormality nor the form of clinical presentation was related to the development of residual damage. The period of exposure to antigen after symptoms developed and the age of the patient appeared to be the most important factors determining recovery of lung function.", "PMID": 970736}
{"id": "pubmed23n0032_8163", "title": "Study of the surface secretion of the bronchiole using radioautography.", "content": "The purpose of the present study was to obtain further information regarding the chemical nature and cell of origin of the surface secretion of the bronchiole using radioautography after the injection of labeled leucine, glucosamine, galactose. Rat lungs were perfused with mammalian Ringer's solution with added albumin. A 10-min pulse of L-leucine labeled with hydrogen-3 was administered, followed by perfusion with nonradioactive solution for 15 min to 4 hours. Similar studies were performed with [3H] glucosamine and [3H] galactose. Heavy labeling of the Clara cells of bronchioles was obtained after injection of [3H] L-leucine. Labeling of alveolar wall cells was also obtained. Labeling of the surface of the bronchiole was much heavier than the labeling of the surface of the alveoli. No specific labeling of bronchiolar cells was obtained with [3H] glucosamine and [3H] galactose. The results provide added evidence for the protein nature of the surface layer of the bronchiole and for the Clara cell as its cell of origin.", "contents": "Study of the surface secretion of the bronchiole using radioautography. The purpose of the present study was to obtain further information regarding the chemical nature and cell of origin of the surface secretion of the bronchiole using radioautography after the injection of labeled leucine, glucosamine, galactose. Rat lungs were perfused with mammalian Ringer's solution with added albumin. A 10-min pulse of L-leucine labeled with hydrogen-3 was administered, followed by perfusion with nonradioactive solution for 15 min to 4 hours. Similar studies were performed with [3H] glucosamine and [3H] galactose. Heavy labeling of the Clara cells of bronchioles was obtained after injection of [3H] L-leucine. Labeling of alveolar wall cells was also obtained. Labeling of the surface of the bronchiole was much heavier than the labeling of the surface of the alveoli. No specific labeling of bronchiolar cells was obtained with [3H] glucosamine and [3H] galactose. The results provide added evidence for the protein nature of the surface layer of the bronchiole and for the Clara cell as its cell of origin.", "PMID": 970737}
{"id": "pubmed23n0032_8164", "title": "Lung volumes and compliance in myasthenia gravis and reversible airway obstruction.", "content": "A patient with myasthenia gravis, reversible airway obstruction, and a vertical chest wall pressure-volume curve was shown to inflate his lungs to 12 liter during the night. During the day, either spontaneously or after isoproterenol, his functional residual capacity decreased by more than 2 liter. The large lung volume changes occurred without a change in slope of the static pressure-volume curve of the lung above functional residual capacity. The changes in the position of this curve might be due to the the very slow filling and emptying of bullae or to unexplained alterations in the length-tension characteristics of the alveoli. The vertical pressure-volume curve of the chest wall suggested that, unlike other patients who have normal or decreased chest wall compliance, this patient's chest wal was not a factor that limited ventilation at high lung volumes.", "contents": "Lung volumes and compliance in myasthenia gravis and reversible airway obstruction. A patient with myasthenia gravis, reversible airway obstruction, and a vertical chest wall pressure-volume curve was shown to inflate his lungs to 12 liter during the night. During the day, either spontaneously or after isoproterenol, his functional residual capacity decreased by more than 2 liter. The large lung volume changes occurred without a change in slope of the static pressure-volume curve of the lung above functional residual capacity. The changes in the position of this curve might be due to the the very slow filling and emptying of bullae or to unexplained alterations in the length-tension characteristics of the alveoli. The vertical pressure-volume curve of the chest wall suggested that, unlike other patients who have normal or decreased chest wall compliance, this patient's chest wal was not a factor that limited ventilation at high lung volumes.", "PMID": 970739}
{"id": "pubmed23n0032_8165", "title": "Postoperative impairment of mucous transport in the lung.", "content": "Impairment of mucous transport is considered significant to the postoperative development of atelectasis, but the association has never beed demonstrated in humans. Tantalum powder, which adheres to airway mucus, can be used to study mucociliary transport. The postoperative clearance of insufflated tantalum powder (mean diameter, 2.5 mum) was investigated in 25 patients. Eighteen patients underwent intra-abdominal vascular surgery and 7 undersent lower-extremity orthopedic procedures. At the completion of surgery, tantalum was insufflated into both lungs of each patient to outline a representative sample of airways from the trachea to the small bronchi. Tantalum clearance was evaluated from serial radiographs obtained immediately after  insufflation, at approximately 6, 18, 26, and 48 hours later, and thereafter whenever appropriate. In the 7 orthopedic patients, clearance of tantalum was progressive and usually complete within 48 hours. Atelectasis did not occur in this group. In 14 of the 18 patients who had abdominal surgery, mucociliary clearance was markedly abnormal in that tantalum was retained for up to 6 days. Pooling of tantalum-labeled mucus occurred in dependent bronchi in 16 of these 18 patients. Pooling preceded and always accompanied radiographically visible atelectasis. Lobar atelectasis occurred in 6 patients and segmental atelectasis in 8. Tantalum-labeled mucus moved peripherally in atelectatic lobes or segments and was retained in these bronchi until re-expansion took place. Thus, impaired ciliary function and mucous transport are associated with and implicated in postoperative pulmonary atelectasis.", "contents": "Postoperative impairment of mucous transport in the lung. Impairment of mucous transport is considered significant to the postoperative development of atelectasis, but the association has never beed demonstrated in humans. Tantalum powder, which adheres to airway mucus, can be used to study mucociliary transport. The postoperative clearance of insufflated tantalum powder (mean diameter, 2.5 mum) was investigated in 25 patients. Eighteen patients underwent intra-abdominal vascular surgery and 7 undersent lower-extremity orthopedic procedures. At the completion of surgery, tantalum was insufflated into both lungs of each patient to outline a representative sample of airways from the trachea to the small bronchi. Tantalum clearance was evaluated from serial radiographs obtained immediately after  insufflation, at approximately 6, 18, 26, and 48 hours later, and thereafter whenever appropriate. In the 7 orthopedic patients, clearance of tantalum was progressive and usually complete within 48 hours. Atelectasis did not occur in this group. In 14 of the 18 patients who had abdominal surgery, mucociliary clearance was markedly abnormal in that tantalum was retained for up to 6 days. Pooling of tantalum-labeled mucus occurred in dependent bronchi in 16 of these 18 patients. Pooling preceded and always accompanied radiographically visible atelectasis. Lobar atelectasis occurred in 6 patients and segmental atelectasis in 8. Tantalum-labeled mucus moved peripherally in atelectatic lobes or segments and was retained in these bronchi until re-expansion took place. Thus, impaired ciliary function and mucous transport are associated with and implicated in postoperative pulmonary atelectasis.", "PMID": 970743}
{"id": "pubmed23n0032_8166", "title": "Acute coccidioidal pleural effusion.", "content": "Acute coccidioidal pleural effusions were studied in 28 patients. Coccidioidal pleural effusion appeared to be secondary to direct spread of contigous parenchymal infection, rather than to hematogenous dissemination, in more than 90 per cent of these patients. Only 2 of 28 patients had the concomitant development of disseminated infection, and both patients possessed factors known to predispose to dissemination. Because of the excellent prognosis in most patients, therapy in patients with coccidioidal pleural effusion should be expectant. This is true even when substantial increases occur in complement fixation titers; such elevations were frequent in this series. Cultures of pleural  biopsy specimens were the most rewarding cultural source in this series, being positive in all 8 patients in whom such biopsy specimens were cultured. Dermal hypersensitivity, including erythema nodosum and erythema multiforme, was commin in patients whose clinical course was uncomplicated.", "contents": "Acute coccidioidal pleural effusion. Acute coccidioidal pleural effusions were studied in 28 patients. Coccidioidal pleural effusion appeared to be secondary to direct spread of contigous parenchymal infection, rather than to hematogenous dissemination, in more than 90 per cent of these patients. Only 2 of 28 patients had the concomitant development of disseminated infection, and both patients possessed factors known to predispose to dissemination. Because of the excellent prognosis in most patients, therapy in patients with coccidioidal pleural effusion should be expectant. This is true even when substantial increases occur in complement fixation titers; such elevations were frequent in this series. Cultures of pleural  biopsy specimens were the most rewarding cultural source in this series, being positive in all 8 patients in whom such biopsy specimens were cultured. Dermal hypersensitivity, including erythema nodosum and erythema multiforme, was commin in patients whose clinical course was uncomplicated.", "PMID": 970744}
{"id": "pubmed23n0032_8167", "title": "Idiopathic pulmonary hemosiderosis: evidence of capillary basement membrane abnormality.", "content": "Electron microscopic examination of a lung biopsy obtained from a child in the active stage of idiopathic pulmonary hemosiderosis disclosed prominent changes in the basement membrane of alveolar capillaries. Review of the pertinent literature showed that a structural defect of lung capillary walls in this disease has been proposed in the past mainly on theoretic grounds but not adequately documented at the ultrastructural level. Concentric multilayering of pulmonary capillary basal lamina, one of the changes observed in our material, is a rare phenomenon in this location, although one that has been well described in extrapulmonary microcirculatory beds and is probably a nonspecific reaction to vascular injury. The conceptual difficulties attending a definition of idiopathic pulmonary hemosiderosis are breifly discussed.", "contents": "Idiopathic pulmonary hemosiderosis: evidence of capillary basement membrane abnormality. Electron microscopic examination of a lung biopsy obtained from a child in the active stage of idiopathic pulmonary hemosiderosis disclosed prominent changes in the basement membrane of alveolar capillaries. Review of the pertinent literature showed that a structural defect of lung capillary walls in this disease has been proposed in the past mainly on theoretic grounds but not adequately documented at the ultrastructural level. Concentric multilayering of pulmonary capillary basal lamina, one of the changes observed in our material, is a rare phenomenon in this location, although one that has been well described in extrapulmonary microcirculatory beds and is probably a nonspecific reaction to vascular injury. The conceptual difficulties attending a definition of idiopathic pulmonary hemosiderosis are breifly discussed.", "PMID": 970745}
{"id": "pubmed23n0032_8168", "title": "High-dose ethambutol: its role in intermittent chemotherapy. A six-year study.", "content": "The outcome of 81 patients receiving supervised ambulatory treatment with twice-weekly isoniazid and ethambutol is reported. The patients were divided into 2 groups, one of which received its entire outpatient treatment with isoniazid and ethambutol, the other receiving isoniazid and ethambutol after initial treatment with isoniazid and streptomycin. In both groups, there was a 100 per cent favorable response at completion of therapy and no relapes detected among those patients followed after treatment. We compared our results with other studies of intermittent-supervised therapy and concluded that twice-weekly isoniazid and ethambutol given up to 18 months after an adequate phase of daily treatment is a highly effective and well-tolerated regimen.", "contents": "High-dose ethambutol: its role in intermittent chemotherapy. A six-year study. The outcome of 81 patients receiving supervised ambulatory treatment with twice-weekly isoniazid and ethambutol is reported. The patients were divided into 2 groups, one of which received its entire outpatient treatment with isoniazid and ethambutol, the other receiving isoniazid and ethambutol after initial treatment with isoniazid and streptomycin. In both groups, there was a 100 per cent favorable response at completion of therapy and no relapes detected among those patients followed after treatment. We compared our results with other studies of intermittent-supervised therapy and concluded that twice-weekly isoniazid and ethambutol given up to 18 months after an adequate phase of daily treatment is a highly effective and well-tolerated regimen.", "PMID": 970746}
{"id": "pubmed23n0032_8169", "title": "A comparison of pulmonary function in male smokers and nonsmokers.", "content": "Results of certain tests of pulmonary function, including a questionnaire, single-breath N2 test of closing capacity, forced expiration, and diffusing capacity were significantly different in groups of male smokers and nonsmokers. The influence of age on these smoking-related changes of pulmonary function was evaluated. The analyses indicated that (1) some tests including number of symptoms; closing capacity, i.e., closing volume plus residual volume as a percentage of total lung capacity; residual volume as a percentage of total lung capacity; Phase III of the single-breath N2 test, and steady-state diffusing capacity (ml of CO/mm Hg - min) revealed significant differences between adjusted mean smoker and nonsmoker values but did not reveal differences associated with age. (2) Tests of forced expiration (1-sec forced expiratory volume/vital capapity, reciprocal of the maximal mid-expiratory flow, maximal flow at 50 per cent of vital capacity; and moments) however, revealed differences between smoker and nonsmoker means )adjusted and unadjusted), as well as increasing smoker-nonsmoker differences with increasing age. It is suggested that the first group of tests probably measured an all-or-none response that occurred with the onset of smoking and was not affected by duration of smoking. The second group of tests probably measured the effects of continued smoking and indicated increasing abnormality associated with longer exposure (years of smoking). Test showing age-related differences between smokers and nonsmokers may reflect cummulative, irreversible changes in pulmonary function to a greater extent than test that do not.", "contents": "A comparison of pulmonary function in male smokers and nonsmokers. Results of certain tests of pulmonary function, including a questionnaire, single-breath N2 test of closing capacity, forced expiration, and diffusing capacity were significantly different in groups of male smokers and nonsmokers. The influence of age on these smoking-related changes of pulmonary function was evaluated. The analyses indicated that (1) some tests including number of symptoms; closing capacity, i.e., closing volume plus residual volume as a percentage of total lung capacity; residual volume as a percentage of total lung capacity; Phase III of the single-breath N2 test, and steady-state diffusing capacity (ml of CO/mm Hg - min) revealed significant differences between adjusted mean smoker and nonsmoker values but did not reveal differences associated with age. (2) Tests of forced expiration (1-sec forced expiratory volume/vital capapity, reciprocal of the maximal mid-expiratory flow, maximal flow at 50 per cent of vital capacity; and moments) however, revealed differences between smoker and nonsmoker means )adjusted and unadjusted), as well as increasing smoker-nonsmoker differences with increasing age. It is suggested that the first group of tests probably measured an all-or-none response that occurred with the onset of smoking and was not affected by duration of smoking. The second group of tests probably measured the effects of continued smoking and indicated increasing abnormality associated with longer exposure (years of smoking). Test showing age-related differences between smokers and nonsmokers may reflect cummulative, irreversible changes in pulmonary function to a greater extent than test that do not.", "PMID": 970747}
{"id": "pubmed23n0032_8170", "title": "Lung function changes in smokers with normal conventional spirometry.", "content": "The comparative ability to detect early abnormalities in smokers by commonly used lung function tests was studied. Sixty-five healthy male nonsmokers served as a reference group and provided standards for 1-sec forced expiratory volume, vital capacity, end-tidal spirometry, spirometric forced mid-and end-expiratory flows, single-breath diffusing capacity, static lung volumes (helium method), and single-breath N2 closing volume measurements, In the present series of 80 male smokers, the measurements of forced mid-expiratory flow and forced end-expiratory flow did not improve the ability of the more conventional indices, 1-sec forced expiratory volume and the ratio of 1-sec forced expiratory volume to vital capacity, to detect obstructive lung disease. In 71 smokers with normal 1-sec forced expiratory volume and ratio of 1-sec forced expiratory volume to vital capacity, the end-tidal spirometry, diffusing capacity, and residual volume indices revealed 14,20, and 21 per cent of abnormalities. respectively. The single-breath N2 closing volume test (Phase IV/vital capacity and slope of Phase III) detected the greatest number of subtle changes in lung function; this was abnormal in 32 per cent of smokers with normal conventional spirometry. In young or light smokers, Phase IV/vital capacity was more frequently increased than the slope of Phase III; an incerse trend was observed in older or heavier smokers. The single-breath N2 closing volume test also provided the greatest number of abnormal results when other indices were impaired in the same subjects.", "contents": "Lung function changes in smokers with normal conventional spirometry. The comparative ability to detect early abnormalities in smokers by commonly used lung function tests was studied. Sixty-five healthy male nonsmokers served as a reference group and provided standards for 1-sec forced expiratory volume, vital capacity, end-tidal spirometry, spirometric forced mid-and end-expiratory flows, single-breath diffusing capacity, static lung volumes (helium method), and single-breath N2 closing volume measurements, In the present series of 80 male smokers, the measurements of forced mid-expiratory flow and forced end-expiratory flow did not improve the ability of the more conventional indices, 1-sec forced expiratory volume and the ratio of 1-sec forced expiratory volume to vital capacity, to detect obstructive lung disease. In 71 smokers with normal 1-sec forced expiratory volume and ratio of 1-sec forced expiratory volume to vital capacity, the end-tidal spirometry, diffusing capacity, and residual volume indices revealed 14,20, and 21 per cent of abnormalities. respectively. The single-breath N2 closing volume test (Phase IV/vital capacity and slope of Phase III) detected the greatest number of subtle changes in lung function; this was abnormal in 32 per cent of smokers with normal conventional spirometry. In young or light smokers, Phase IV/vital capacity was more frequently increased than the slope of Phase III; an incerse trend was observed in older or heavier smokers. The single-breath N2 closing volume test also provided the greatest number of abnormal results when other indices were impaired in the same subjects.", "PMID": 970748}
{"id": "pubmed23n0032_8171", "title": "Transit time analysis of the forced expiratory vital capacity in cystic fibrosis.", "content": "Transit time analysis of the forced expiratory vital capacity maneuver was applied to 37 patients with cystic fibrosis 8 to 22 years of age. This analysis divides the vital capacity into segments of air and assigns a transit time to each segment. The characteristics of the distribution of these transit times are used as measurements of pulmonary function. The quantities were compared with the forced vital capacity, 1-sec forced expiratory volume, ratio of 1-sec forced expiratory volume to vital capacity, peak flow, forced expiratory flow during the middle half of the forced vital capacity, maximal expiratory flow at 25 per cent of the vital capacity, specific airway conductance, and arterial PO2 of these patients. The standard deviation of the transit times was the most frequently abnormal. The mean transit time had the largest range of values in terms of its own standard deviation, and it was the best single estimate of over-all lung function because it correlated almost equally with large and small airway function. The coefficient of cariance of transit times was specific in detecting abnormality of small airways and was as sensitive in the detection of minimal lung disease as the standard deviation of transit times.", "contents": "Transit time analysis of the forced expiratory vital capacity in cystic fibrosis. Transit time analysis of the forced expiratory vital capacity maneuver was applied to 37 patients with cystic fibrosis 8 to 22 years of age. This analysis divides the vital capacity into segments of air and assigns a transit time to each segment. The characteristics of the distribution of these transit times are used as measurements of pulmonary function. The quantities were compared with the forced vital capacity, 1-sec forced expiratory volume, ratio of 1-sec forced expiratory volume to vital capacity, peak flow, forced expiratory flow during the middle half of the forced vital capacity, maximal expiratory flow at 25 per cent of the vital capacity, specific airway conductance, and arterial PO2 of these patients. The standard deviation of the transit times was the most frequently abnormal. The mean transit time had the largest range of values in terms of its own standard deviation, and it was the best single estimate of over-all lung function because it correlated almost equally with large and small airway function. The coefficient of cariance of transit times was specific in detecting abnormality of small airways and was as sensitive in the detection of minimal lung disease as the standard deviation of transit times.", "PMID": 970749}
{"id": "pubmed23n0032_8172", "title": "The effect of lung volume on the ventilatory response to CO2 during resistive loading in normal subjects.", "content": "The ventilatory response to CO2 during resistive loading was determined in normal subjects when the end-expiratory level was allowed to increase and when it was restored to that present in the unloaded state. Restoration of the end-expiratory level of that seen in the unloaded state, during the resistive loading, resulted in an increase in ventilatory response to CO2. The mechanism by which increases in lung volume affect the ventilatory response to CO2 is unknown; multiple factors are likely to be involved. It is suggested that an increase in end-expiratory level plays a major role in limiting the ventilatory response to CO2 during resistive loading and may also be an important factor in the reduced ventilatory response to CO2 that is seen clinically when there is obstruction to air flow.", "contents": "The effect of lung volume on the ventilatory response to CO2 during resistive loading in normal subjects. The ventilatory response to CO2 during resistive loading was determined in normal subjects when the end-expiratory level was allowed to increase and when it was restored to that present in the unloaded state. Restoration of the end-expiratory level of that seen in the unloaded state, during the resistive loading, resulted in an increase in ventilatory response to CO2. The mechanism by which increases in lung volume affect the ventilatory response to CO2 is unknown; multiple factors are likely to be involved. It is suggested that an increase in end-expiratory level plays a major role in limiting the ventilatory response to CO2 during resistive loading and may also be an important factor in the reduced ventilatory response to CO2 that is seen clinically when there is obstruction to air flow.", "PMID": 970750}
{"id": "pubmed23n0032_8173", "title": "Effects of cord factor on liver lipid: accumulation triglycerides.", "content": "A single intraperitoneal injection of cord factor induced a remarkable increase in the amounts of triglycerides in liver. This effect was specific for cord factor among various lipid fractions of tuberclebacilli. Other lipid species of liver than triglycerides were not affected by cord factor injection. The accumulation of triglycerides became evident 16 to 24 hours after the cord factor injection and reached the maximum at 48 hours. As small a quantity as 12.5 mug of cord factor effectively induced the increase of triglycerides in liver.", "contents": "Effects of cord factor on liver lipid: accumulation triglycerides. A single intraperitoneal injection of cord factor induced a remarkable increase in the amounts of triglycerides in liver. This effect was specific for cord factor among various lipid fractions of tuberclebacilli. Other lipid species of liver than triglycerides were not affected by cord factor injection. The accumulation of triglycerides became evident 16 to 24 hours after the cord factor injection and reached the maximum at 48 hours. As small a quantity as 12.5 mug of cord factor effectively induced the increase of triglycerides in liver.", "PMID": 970751}
{"id": "pubmed23n0032_8174", "title": "Analysis of the causes of mortality from appendicitis.", "content": "An analysis of 944 cases of acute appendicitis treated at the University of Kentucky Medical Center from 1966 to 1973 is presented. There were 12 deaths in this series, a mortality rate of 1.3 per cent, and the clinical presentation, operative and autopsy findings are discussed. It is significant that seven of the 12 patients had other serious illnesses that influenced the decision for operation and that operation was delayed over 48 hours in 50 percent. It is stressed that the need for early exploration in the patient suspected of having appendicitis is even more imperative in the individual with concomitant disease. Adequate but immediate preoperative preparation with fluids and electrolytes antibiotics and stabilization of renal, pulmonary and cardiac status is mandatory.", "contents": "Analysis of the causes of mortality from appendicitis. An analysis of 944 cases of acute appendicitis treated at the University of Kentucky Medical Center from 1966 to 1973 is presented. There were 12 deaths in this series, a mortality rate of 1.3 per cent, and the clinical presentation, operative and autopsy findings are discussed. It is significant that seven of the 12 patients had other serious illnesses that influenced the decision for operation and that operation was delayed over 48 hours in 50 percent. It is stressed that the need for early exploration in the patient suspected of having appendicitis is even more imperative in the individual with concomitant disease. Adequate but immediate preoperative preparation with fluids and electrolytes antibiotics and stabilization of renal, pulmonary and cardiac status is mandatory.", "PMID": 970756}
{"id": "pubmed23n0032_8175", "title": "Surgical management of parathyroid disease.", "content": "One hundred fifty patients who had operation for hyperparathyroidism at the National Institutes of Health are reviewed. The series is composed of several groups of patients, including 18 with normocalcemic hyperparathyroidism, 13 with hyperparathyroidism associated with MEA II, and 24 patients with a history of previous parathyroid operation. Selective venous catherization and radioimmunoassay of parathormone was carried out in 81 patients and was interpreted as being localizing in 65 cases. Following operation, six patients remained hypercalcemic. There were nine instances of permanent hypocalcemia and four cases in which permanent recurrent nerve injuries were experienced. The operative management of these patients is outlined in detail, stressing an approach which includes the surgeon, the internist and the pathologist. Based upon pathological evidence, the importance of identifying by frozen section four glands is emphasized.", "contents": "Surgical management of parathyroid disease. One hundred fifty patients who had operation for hyperparathyroidism at the National Institutes of Health are reviewed. The series is composed of several groups of patients, including 18 with normocalcemic hyperparathyroidism, 13 with hyperparathyroidism associated with MEA II, and 24 patients with a history of previous parathyroid operation. Selective venous catherization and radioimmunoassay of parathormone was carried out in 81 patients and was interpreted as being localizing in 65 cases. Following operation, six patients remained hypercalcemic. There were nine instances of permanent hypocalcemia and four cases in which permanent recurrent nerve injuries were experienced. The operative management of these patients is outlined in detail, stressing an approach which includes the surgeon, the internist and the pathologist. Based upon pathological evidence, the importance of identifying by frozen section four glands is emphasized.", "PMID": 970757}
{"id": "pubmed23n0032_8176", "title": "Acute jejunogastric intussusception.", "content": "Our patient represents an example of acute retrograde jejunograstric intussusception with the additional finding of a bleeding jejunal ulcer in the intussusception. The ulcer may have been etiological in precipitating the intussusception, or a consequence of mucosal sloughing in the hemorrhagic intussusception. Early diagnosis and immediate surgical therapy resulted in an uneventful recovery.", "contents": "Acute jejunogastric intussusception. Our patient represents an example of acute retrograde jejunograstric intussusception with the additional finding of a bleeding jejunal ulcer in the intussusception. The ulcer may have been etiological in precipitating the intussusception, or a consequence of mucosal sloughing in the hemorrhagic intussusception. Early diagnosis and immediate surgical therapy resulted in an uneventful recovery.", "PMID": 970758}
{"id": "pubmed23n0032_8177", "title": "Effect of antibiotics on the in vitro neutrophil chemotactic response.", "content": "Polymorphonuclear neutrophilic leukocyte chemotaxis was examined in vitro in the presence of the following antibiotics: tetracycline, chloramphenicol, erythromycin, gentamicin, methicillin, sulfadiazine, cephalothin, oxacillin, nafcillin and clindamycin. Results indicate that high concentrations (1 mg/ml) of tetracycline, nafcillin, erythromycin and sulfadiazine inhibited the chemotactic response of neutrophils. Tetracycline produced an inhibition at concentrations as low as 1mug/ml. A stimulation of the neutrophil chemotactic response was observed in the presence of chloramphenicol in all concentrations. The results are discussed with particular emphasis on the implications to host immunity.", "contents": "Effect of antibiotics on the in vitro neutrophil chemotactic response. Polymorphonuclear neutrophilic leukocyte chemotaxis was examined in vitro in the presence of the following antibiotics: tetracycline, chloramphenicol, erythromycin, gentamicin, methicillin, sulfadiazine, cephalothin, oxacillin, nafcillin and clindamycin. Results indicate that high concentrations (1 mg/ml) of tetracycline, nafcillin, erythromycin and sulfadiazine inhibited the chemotactic response of neutrophils. Tetracycline produced an inhibition at concentrations as low as 1mug/ml. A stimulation of the neutrophil chemotactic response was observed in the presence of chloramphenicol in all concentrations. The results are discussed with particular emphasis on the implications to host immunity.", "PMID": 970760}
{"id": "pubmed23n0032_8178", "title": "Perforation of the normal colon during barium contrast examination.", "content": "Two cases of perforation of the normal colon during barium contrast examination are reported. Overdistention of the colon with air and barium contrast examination are reported. Overdistention of the colon with air and barium should be avoided in a patient with a competent ileocecal value. While rare, this complication can be life-threatening.", "contents": "Perforation of the normal colon during barium contrast examination. Two cases of perforation of the normal colon during barium contrast examination are reported. Overdistention of the colon with air and barium contrast examination are reported. Overdistention of the colon with air and barium should be avoided in a patient with a competent ileocecal value. While rare, this complication can be life-threatening.", "PMID": 970761}
{"id": "pubmed23n0032_8179", "title": "Spontaneous perforation of the extrahepatic bile ducts in infancy.", "content": "An additional case of spontaneous perforation of the bile duct in infancy is reported. Review of the literature reveals a characteristic history and physical findings in all infants with this entity. Treatment should be prompt and should consist of simple drainage of the area of perforation. Recovery is to be expected in all those patients in whom bile is sterile.", "contents": "Spontaneous perforation of the extrahepatic bile ducts in infancy. An additional case of spontaneous perforation of the bile duct in infancy is reported. Review of the literature reveals a characteristic history and physical findings in all infants with this entity. Treatment should be prompt and should consist of simple drainage of the area of perforation. Recovery is to be expected in all those patients in whom bile is sterile.", "PMID": 970763}
{"id": "pubmed23n0032_8180", "title": "Sepsis with a new species of Corynebacterium.", "content": "Sepsis with a previously undescribed species of Corynebacterium was documented in four patients. All patients had predisposing illness at the time of infection, three patients having leukemia in relapse and one having a porencephalic cyst and a ventriculoatrial shunt. The isolates from blood cultures had a characteristic metallic sheen when grown on blood agar. They were resistant to most antibiotics tested, including the penicillins, but were uniformly sensitive to vancomycin. Common biochemical characteristics, the metallic sheen, and the unusual antibiotic sensitivity pattern suggest that these isolates comprise a new species or group of closely related species of Corynebacterium that is capable of infection in man.", "contents": "Sepsis with a new species of Corynebacterium. Sepsis with a previously undescribed species of Corynebacterium was documented in four patients. All patients had predisposing illness at the time of infection, three patients having leukemia in relapse and one having a porencephalic cyst and a ventriculoatrial shunt. The isolates from blood cultures had a characteristic metallic sheen when grown on blood agar. They were resistant to most antibiotics tested, including the penicillins, but were uniformly sensitive to vancomycin. Common biochemical characteristics, the metallic sheen, and the unusual antibiotic sensitivity pattern suggest that these isolates comprise a new species or group of closely related species of Corynebacterium that is capable of infection in man.", "PMID": 970766}
{"id": "pubmed23n0032_8181", "title": "Absence of complete homologous immunity in hepatitis B infection after massive exposure.", "content": "Evidence from prospective studies of human cases of posttransfusion hepatitis observed among 299 cardiac surgery patients, and from each of three experimentally challenged chimpanzees, indicates that immunity to hepatitis B infection can be overcome by massive challenge doses. However, all cases of hepatitis in patients with prior immunity have been subclinical. Chimpanzees developed hepatitis and HBs antigenemia when challenged with hepatitis B virus that had an antigenic subtype identical to that used in the primary infection. This response provides evidence against the role of differences in antigenic composition of initial and challenge strains of virus as an explanation for incomplete homologous immunity.", "contents": "Absence of complete homologous immunity in hepatitis B infection after massive exposure. Evidence from prospective studies of human cases of posttransfusion hepatitis observed among 299 cardiac surgery patients, and from each of three experimentally challenged chimpanzees, indicates that immunity to hepatitis B infection can be overcome by massive challenge doses. However, all cases of hepatitis in patients with prior immunity have been subclinical. Chimpanzees developed hepatitis and HBs antigenemia when challenged with hepatitis B virus that had an antigenic subtype identical to that used in the primary infection. This response provides evidence against the role of differences in antigenic composition of initial and challenge strains of virus as an explanation for incomplete homologous immunity.", "PMID": 970767}
{"id": "pubmed23n0032_8182", "title": "Felty's syndrome: granulocyte-bound immunoglobulin G and splenectomy.", "content": "A quantitative antiglobulin consumption technique was used to measure immunoglobulin G (IgG) present on human granulocytes. Granulocytes from 50 normal subjects had less than 20 X 10(-14) g IgG per cell. Patients with granulocytopenia due to bone-marrow failure, patients with IgG multiple myeloma, patients with splenomegaly, and patients with rheumatoid arthritis without granulocytopenia had granulocyte-bound IgG within the range of normal. Four patients with rheumatoid arthritis, splenomegaly, and severe granulocytopenia (Felty's syndrome) had granulocyte-bound IgG between 30 and 220 X 10(-14) g IgG per cell. One of these patients underwent splenectomy, after which his granulocyte-bound IgG fell to normal. Seven additional patients with Felty's syndrome who had previously undergone splenectomy had normal levels of granulocyte-bound IgG. Thus quantitation of granulocyte-bound IgG appears to be useful in defining patients with immunologically mediated granulocytopenia. Studies of patients with Felty's syndrome who have undergone splenectomy suggest that the spleen may produce this neutrophile-bound immunoglobulin.", "contents": "Felty's syndrome: granulocyte-bound immunoglobulin G and splenectomy. A quantitative antiglobulin consumption technique was used to measure immunoglobulin G (IgG) present on human granulocytes. Granulocytes from 50 normal subjects had less than 20 X 10(-14) g IgG per cell. Patients with granulocytopenia due to bone-marrow failure, patients with IgG multiple myeloma, patients with splenomegaly, and patients with rheumatoid arthritis without granulocytopenia had granulocyte-bound IgG within the range of normal. Four patients with rheumatoid arthritis, splenomegaly, and severe granulocytopenia (Felty's syndrome) had granulocyte-bound IgG between 30 and 220 X 10(-14) g IgG per cell. One of these patients underwent splenectomy, after which his granulocyte-bound IgG fell to normal. Seven additional patients with Felty's syndrome who had previously undergone splenectomy had normal levels of granulocyte-bound IgG. Thus quantitation of granulocyte-bound IgG appears to be useful in defining patients with immunologically mediated granulocytopenia. Studies of patients with Felty's syndrome who have undergone splenectomy suggest that the spleen may produce this neutrophile-bound immunoglobulin.", "PMID": 970768}
{"id": "pubmed23n0032_8183", "title": "Sicca syndrome and total lipodystrophy: a case in a fifteen-year-old female patient.", "content": "A 15-year-old girl is described with the sicca syndrome of keratoconjunctivitis and xerostomia. There was no associated rheumatoid arthritis or other connective tissue disorder, but total lipodystrophy had been present since the age of 9 years. Marked hyperreactivity of the humoral immune system and suppression of cell-mediated immunity was shown. The association of sicca syndrome with total lipodystrophy has not previously been reported.", "contents": "Sicca syndrome and total lipodystrophy: a case in a fifteen-year-old female patient. A 15-year-old girl is described with the sicca syndrome of keratoconjunctivitis and xerostomia. There was no associated rheumatoid arthritis or other connective tissue disorder, but total lipodystrophy had been present since the age of 9 years. Marked hyperreactivity of the humoral immune system and suppression of cell-mediated immunity was shown. The association of sicca syndrome with total lipodystrophy has not previously been reported.", "PMID": 970769}
{"id": "pubmed23n0032_8184", "title": "Menopause and risk of cardiovascular disease: the Framingham study.", "content": "The relation of menopause to cardiovascular disease incidence was examined in women less than 55 years old from the cohort of 2873 women in the initial Framingham examination. Although the number of person-years of experience during the 20 years of observation was nearly the same for premenopausal and postmenopausal status, there were only 20 cardiovascular events among the premenopausal women in this age group whereas 70 events occurred among the postmenopausal women of the same age. In each specific age group studied incidence rates were lower in premenopausal than postmenopausal women. This was also true for coronary heart disease. Contrast for \"hard\" diagnoses of cardiovascular disease (excluding diagnoses of angina pectoris and intermittent claudication) was in the same direction. Although cholesterol and hemoglobin did rise somewhat more steeply in women undergoing the menopause, this greater incidence of cardiovascular disease in postmenopausal women could not be explained by the influence of the menopause on the usual cardiovascular risk factors.", "contents": "Menopause and risk of cardiovascular disease: the Framingham study. The relation of menopause to cardiovascular disease incidence was examined in women less than 55 years old from the cohort of 2873 women in the initial Framingham examination. Although the number of person-years of experience during the 20 years of observation was nearly the same for premenopausal and postmenopausal status, there were only 20 cardiovascular events among the premenopausal women in this age group whereas 70 events occurred among the postmenopausal women of the same age. In each specific age group studied incidence rates were lower in premenopausal than postmenopausal women. This was also true for coronary heart disease. Contrast for \"hard\" diagnoses of cardiovascular disease (excluding diagnoses of angina pectoris and intermittent claudication) was in the same direction. Although cholesterol and hemoglobin did rise somewhat more steeply in women undergoing the menopause, this greater incidence of cardiovascular disease in postmenopausal women could not be explained by the influence of the menopause on the usual cardiovascular risk factors.", "PMID": 970770}
{"id": "pubmed23n0032_8185", "title": "Bradycardia with mitral valve prolapse: a potential mechanism of sudden death.", "content": "Eleven members of a family with a high prevalence of mitral valve prolapse were investigated. Seven had documented sinus bradycardia, and five had mitral valve prolapse. Three patients with both mitral valve prolapse and bradycardia had recurrent syncope reproduced by simple head-up tilting, and in one patient this resulted in asystole. The hemodynamic response to isoproterenol and phenylephrine administration were normal. Supine plasma norepinephrine levels were normal in all three and increased appropriately in two of three patients after tilting. Atrial pacing studies documented marked prolongation of atrial-His intervals and inability to maintain 1:1 atrioventricular conduction when paced at a rate of 120/min. These findings were reversed by atropine. This family shows a close correlation between mitral valve prolapse and potentially lethal bradycardia. Excessive vagal tone is believed to be responsible for both bradycardia and sinus arrest, which in two patients was prevented by permanent demand pacing.", "contents": "Bradycardia with mitral valve prolapse: a potential mechanism of sudden death. Eleven members of a family with a high prevalence of mitral valve prolapse were investigated. Seven had documented sinus bradycardia, and five had mitral valve prolapse. Three patients with both mitral valve prolapse and bradycardia had recurrent syncope reproduced by simple head-up tilting, and in one patient this resulted in asystole. The hemodynamic response to isoproterenol and phenylephrine administration were normal. Supine plasma norepinephrine levels were normal in all three and increased appropriately in two of three patients after tilting. Atrial pacing studies documented marked prolongation of atrial-His intervals and inability to maintain 1:1 atrioventricular conduction when paced at a rate of 120/min. These findings were reversed by atropine. This family shows a close correlation between mitral valve prolapse and potentially lethal bradycardia. Excessive vagal tone is believed to be responsible for both bradycardia and sinus arrest, which in two patients was prevented by permanent demand pacing.", "PMID": 970771}
{"id": "pubmed23n0032_8186", "title": "\"e\" Antigen, Dane particles, and serum DNA polymerase activity in HBsAg carriers.", "content": "Sera of 103 carriers of hepatitis B surface antigen were assayed for e-antigen and anti-e. Twenty-four were e-antigen-positive, 31 anti-e-positive, and 48 had neither detectable (e-negative). Aminotransferases were elevated in 75% of the e-antigen-positive carriers compared with 25% of e-negative carriers (P less than 0.001) and 13% of anti-e-positive carriers (P less than 0.001). Serum DNA polymerase activity was significantly higher in the e-antigen-positive carriers than in carriers without e-antigen. Dane particles were shown in 10 of 12 carriers with e-antigen, compared with one of 12 e-negative carriers (P less than 0.0003) and none of 12 anti-e-positive carriers (P less than 0.00003). These results suggest that ongoing hepatitis B viral replication is more active in e-antigen-positive carriers than in carriers without e-antigen, a finding that may help explain the high prevalence of chronic active hepatitis described in these individuals.", "contents": "\"e\" Antigen, Dane particles, and serum DNA polymerase activity in HBsAg carriers. Sera of 103 carriers of hepatitis B surface antigen were assayed for e-antigen and anti-e. Twenty-four were e-antigen-positive, 31 anti-e-positive, and 48 had neither detectable (e-negative). Aminotransferases were elevated in 75% of the e-antigen-positive carriers compared with 25% of e-negative carriers (P less than 0.001) and 13% of anti-e-positive carriers (P less than 0.001). Serum DNA polymerase activity was significantly higher in the e-antigen-positive carriers than in carriers without e-antigen. Dane particles were shown in 10 of 12 carriers with e-antigen, compared with one of 12 e-negative carriers (P less than 0.0003) and none of 12 anti-e-positive carriers (P less than 0.00003). These results suggest that ongoing hepatitis B viral replication is more active in e-antigen-positive carriers than in carriers without e-antigen, a finding that may help explain the high prevalence of chronic active hepatitis described in these individuals.", "PMID": 970772}
{"id": "pubmed23n0032_8187", "title": "Aerobic and anaerobic bacteria in diabetic foot ulcers.", "content": "Twenty diabetic foot ulcers were cultured using optimal microbiologic techniques. Anaerobic bacteria coexisted with the more commonly recognized aerobic bacteria in 18 specimens. There were a total of 116 isolates with an average of 5.8 species per specimen (3.2 aerobes and 2.6 anaerobes). The principal isolates were Bacteroides species (sp.) (17 strains), peptococci (16), Proteus sp. (11), enterococci (9), Staphylococcus aureus (7), clostridia (7), and Escherichia coli (6). When antimicrobial therapy is indicated the selection of agents should consider the likelihood of a complex aerobic-anaerobic flora.", "contents": "Aerobic and anaerobic bacteria in diabetic foot ulcers. Twenty diabetic foot ulcers were cultured using optimal microbiologic techniques. Anaerobic bacteria coexisted with the more commonly recognized aerobic bacteria in 18 specimens. There were a total of 116 isolates with an average of 5.8 species per specimen (3.2 aerobes and 2.6 anaerobes). The principal isolates were Bacteroides species (sp.) (17 strains), peptococci (16), Proteus sp. (11), enterococci (9), Staphylococcus aureus (7), clostridia (7), and Escherichia coli (6). When antimicrobial therapy is indicated the selection of agents should consider the likelihood of a complex aerobic-anaerobic flora.", "PMID": 970773}
{"id": "pubmed23n0032_8188", "title": "Chronic ulcerative colitis, skin necrosis, and cryofibrinogenemia.", "content": "Necrotizing skin lesions developed in a man with chronic ulcerative colitis. No evidence of intrinsic disease of medium or small-sized vessels was found. A circulating cryofibrinogen was thought to be responsible for in situ thrombosis leading to skin infarctions. Sodium warfarin in a daily dose of 2.5 to 5 mg appears to have thwarted progression of developing lesions and the occurrence of new ones.", "contents": "Chronic ulcerative colitis, skin necrosis, and cryofibrinogenemia. Necrotizing skin lesions developed in a man with chronic ulcerative colitis. No evidence of intrinsic disease of medium or small-sized vessels was found. A circulating cryofibrinogen was thought to be responsible for in situ thrombosis leading to skin infarctions. Sodium warfarin in a daily dose of 2.5 to 5 mg appears to have thwarted progression of developing lesions and the occurrence of new ones.", "PMID": 970774}
{"id": "pubmed23n0032_8189", "title": "[Suppurative parietal endocarditides].", "content": "As opposed to acute or subacute orificial localizations, suppurative parietal endocarditis is a very rare entity (5 cases in 3,900 autopsies). More readily localized in the left heart and being generators of systemic emboli, they remain latent until anatomically verified. Two circumstances promote their occurrence : the focal point caused by the mural thrombus of a recent myocardial infarction ; septicemic infections with pulmonary localization and neighbouring thrombophlebitis within the context of intense depression of immunity.", "contents": "[Suppurative parietal endocarditides]. As opposed to acute or subacute orificial localizations, suppurative parietal endocarditis is a very rare entity (5 cases in 3,900 autopsies). More readily localized in the left heart and being generators of systemic emboli, they remain latent until anatomically verified. Two circumstances promote their occurrence : the focal point caused by the mural thrombus of a recent myocardial infarction ; septicemic infections with pulmonary localization and neighbouring thrombophlebitis within the context of intense depression of immunity.", "PMID": 970800}
{"id": "pubmed23n0032_8190", "title": "[Multiple angioleiomyomas of both lungs. Probable hamartomatous tumors. Very late death due to respiratory insufficiency].", "content": "Case presentation : Multiple tumor nodules in both lungs, identified by biopsy and necropsy, in a 48 year old woman, 8 years after radiological discovery ; death due to respiratory failure after long tolerance ; peculiar angio-leiomyomatous type pulmonary tumors. This purely pulmonary tumor disease is difficult to understand ; the most probable hypothesis envisaged is that of hamartomatosis ; comparison with certain general dysplastic disease is possible, and in particular Bourneville's disease and lymphangio-myomatosis.", "contents": "[Multiple angioleiomyomas of both lungs. Probable hamartomatous tumors. Very late death due to respiratory insufficiency]. Case presentation : Multiple tumor nodules in both lungs, identified by biopsy and necropsy, in a 48 year old woman, 8 years after radiological discovery ; death due to respiratory failure after long tolerance ; peculiar angio-leiomyomatous type pulmonary tumors. This purely pulmonary tumor disease is difficult to understand ; the most probable hypothesis envisaged is that of hamartomatosis ; comparison with certain general dysplastic disease is possible, and in particular Bourneville's disease and lymphangio-myomatosis.", "PMID": 970801}
{"id": "pubmed23n0032_8191", "title": "[Language in schizophrenic patients].", "content": "The author tries to analyze the results of research on schizophrenics language during the last thirty years. The almost permanent contradictions of these results leads the author to the conclusion that there is no evidence for a basic disturbance in schizophrenic language. Two hypothesis are proposed to explain the contradictory results 1) the exclusively clinical definition of schizophrenia is of minimal utility as a frame-work for psychometric and linguistic research programs. 2) psychometric and linguistic tests seem to have no reliability to elucidate a phenomenea as complex as the verbal expression of the schizophrenic.", "contents": "[Language in schizophrenic patients]. The author tries to analyze the results of research on schizophrenics language during the last thirty years. The almost permanent contradictions of these results leads the author to the conclusion that there is no evidence for a basic disturbance in schizophrenic language. Two hypothesis are proposed to explain the contradictory results 1) the exclusively clinical definition of schizophrenia is of minimal utility as a frame-work for psychometric and linguistic research programs. 2) psychometric and linguistic tests seem to have no reliability to elucidate a phenomenea as complex as the verbal expression of the schizophrenic.", "PMID": 970814}
{"id": "pubmed23n0032_8192", "title": "[The hyperkinetic child. Psychopathological aspects (author's transl)].", "content": "The hyperkinetic syndrome, such as it is abundantly described in the literature of American authors, is clearly defined by the association in a young child, essentially at the beginning of schooling, of hyper-activity, psycho-motor instability, affective and learning disorders, emotional lability, immaturity, etc. It would appear that psycho-stimulants (in particular amphetamines) bring about a spectacular improvement in these children, representing a therapeutic test that confirms the diagnosis. In America, insistence is placed on the physiopathogeny of the disorders, and on the minimal cerebral dysfunction. This pathology, which, it would appear, does not seem to have been recognised in France, where the psychomotor instability is only one among many symptoms. We report here a synthetic clinical study of hyperkinetic children observed over a period of several years. They are particular in that they were seen at an early age (between 18 months and 4 years old). The psychopathological study of the early stages (which is usually missing in the literature) makes it possible to objectify a preliminary phase dominated by negative signs, of the \"silent series\": depressive and regressive tendencies, later hidden by agitation. We underline the importance of the early distorsion of the mother-child link, which appears to be a determining factor in the appearance of the hyperkinetic syndrome. These date (which tend to contradict many studies) lead one to reject the chemotherapy solution, and in particular psychostimulants for the greater majority of cases, and rather to intervene at an early stage, and ideally speaking preventively, to instigate above all a psychotherapy of the child and often of the mother (co-therapy), to restore a good level of communication, and the mother-child link. This in-depth approach should make it possible to avoid a purely symptomatic treatment, with its uncertain effects, and its often undersirable evolution.", "contents": "[The hyperkinetic child. Psychopathological aspects (author's transl)]. The hyperkinetic syndrome, such as it is abundantly described in the literature of American authors, is clearly defined by the association in a young child, essentially at the beginning of schooling, of hyper-activity, psycho-motor instability, affective and learning disorders, emotional lability, immaturity, etc. It would appear that psycho-stimulants (in particular amphetamines) bring about a spectacular improvement in these children, representing a therapeutic test that confirms the diagnosis. In America, insistence is placed on the physiopathogeny of the disorders, and on the minimal cerebral dysfunction. This pathology, which, it would appear, does not seem to have been recognised in France, where the psychomotor instability is only one among many symptoms. We report here a synthetic clinical study of hyperkinetic children observed over a period of several years. They are particular in that they were seen at an early age (between 18 months and 4 years old). The psychopathological study of the early stages (which is usually missing in the literature) makes it possible to objectify a preliminary phase dominated by negative signs, of the \"silent series\": depressive and regressive tendencies, later hidden by agitation. We underline the importance of the early distorsion of the mother-child link, which appears to be a determining factor in the appearance of the hyperkinetic syndrome. These date (which tend to contradict many studies) lead one to reject the chemotherapy solution, and in particular psychostimulants for the greater majority of cases, and rather to intervene at an early stage, and ideally speaking preventively, to instigate above all a psychotherapy of the child and often of the mother (co-therapy), to restore a good level of communication, and the mother-child link. This in-depth approach should make it possible to avoid a purely symptomatic treatment, with its uncertain effects, and its often undersirable evolution.", "PMID": 970815}
{"id": "pubmed23n0032_8193", "title": "[Paradoxical effects of iodine overload on thyroid function].", "content": "Any chronic iodine overload resulting from long term ingestion of a medication or the administration of a non-resorbable contrast medium, may result in a goitre with or without hypothyroidism, and in particular in patients with a past history of thyroid disease. By contrast, the administration of iodine is no longer indicated in the treatment of enedmic goitres since the hypothesis of an iodine deficiency in the pathogenesis of such goitres may be excluded in most countries, and in particular western countries, and the risk of the development of thyrotoxicosis in such patients is not negligeable. It would thus be desirable that iodine no longer be used in the composition of medications when its presence is not indispensalbe and where, furthermore, it may contribute to developement of an increasingly disturbing iatrogenic complication.", "contents": "[Paradoxical effects of iodine overload on thyroid function]. Any chronic iodine overload resulting from long term ingestion of a medication or the administration of a non-resorbable contrast medium, may result in a goitre with or without hypothyroidism, and in particular in patients with a past history of thyroid disease. By contrast, the administration of iodine is no longer indicated in the treatment of enedmic goitres since the hypothesis of an iodine deficiency in the pathogenesis of such goitres may be excluded in most countries, and in particular western countries, and the risk of the development of thyrotoxicosis in such patients is not negligeable. It would thus be desirable that iodine no longer be used in the composition of medications when its presence is not indispensalbe and where, furthermore, it may contribute to developement of an increasingly disturbing iatrogenic complication.", "PMID": 970803}
{"id": "pubmed23n0032_8194", "title": "[Undifferentiated naso-pharyngeal carcinoma of the Schmincke-Regaud type. Apropos of 3 cases].", "content": "The authors present 3 cases of undifferentiared naso-pharyngeal carcinoma of the Schmincke-Regaud type. They study the anatomopathological, appearances, the geographic distribution, the background, the clinical expression and course, the immunological, virological and genetic factors of this very curious type of cancer the diagnosis and treatment of which remain difficult.", "contents": "[Undifferentiated naso-pharyngeal carcinoma of the Schmincke-Regaud type. Apropos of 3 cases]. The authors present 3 cases of undifferentiared naso-pharyngeal carcinoma of the Schmincke-Regaud type. They study the anatomopathological, appearances, the geographic distribution, the background, the clinical expression and course, the immunological, virological and genetic factors of this very curious type of cancer the diagnosis and treatment of which remain difficult.", "PMID": 970804}
{"id": "pubmed23n0032_8195", "title": "[Midline malignant granuloma and cyclophosphamide].", "content": "One case is presented whose characteristics features were breast hypertrophy, normal immune functions and the efficacy of cyclophosphamide alone : A complete remission is present 31 months after the granuloma started, after 7 and a half months of treatment by cyclophosphamide, 14 months after its interruption by the patient. It is proposed that lethal midline granuloma is a localised form of Wegener's granulomatosis ; between these extreme forms are the limited (pulomonary) form of Wegener's granulomatosis, and the lethal midline granuloma with cutaneous, glandular, digestive or urethrogenital involvements. Therapeutic is briefly reviewed and the efficacy of cyclophosphamide claimed.", "contents": "[Midline malignant granuloma and cyclophosphamide]. One case is presented whose characteristics features were breast hypertrophy, normal immune functions and the efficacy of cyclophosphamide alone : A complete remission is present 31 months after the granuloma started, after 7 and a half months of treatment by cyclophosphamide, 14 months after its interruption by the patient. It is proposed that lethal midline granuloma is a localised form of Wegener's granulomatosis ; between these extreme forms are the limited (pulomonary) form of Wegener's granulomatosis, and the lethal midline granuloma with cutaneous, glandular, digestive or urethrogenital involvements. Therapeutic is briefly reviewed and the efficacy of cyclophosphamide claimed.", "PMID": 970805}
{"id": "pubmed23n0032_8196", "title": "[Fistula between a systemic artery and the pulmonary arterio-venous system. Apropos of a case. Review of the literature].", "content": "The authors present the case of a 19 year old man with a continuous murmurin the right latero-thoracic region, with no functional symptomatology. Complementary examination demonstrated the presence of an angiomatosis which had developed at the expense of the right internal and external mammary vessels, and communicating with the pulmonary arterio-venous system.", "contents": "[Fistula between a systemic artery and the pulmonary arterio-venous system. Apropos of a case. Review of the literature]. The authors present the case of a 19 year old man with a continuous murmurin the right latero-thoracic region, with no functional symptomatology. Complementary examination demonstrated the presence of an angiomatosis which had developed at the expense of the right internal and external mammary vessels, and communicating with the pulmonary arterio-venous system.", "PMID": 970806}
{"id": "pubmed23n0032_8197", "title": "[Suicidal behavior and meterological conditions].", "content": "Six hundred and seventy five cases of attempted suicide observed in a resuscitation department were confronted with a certain number of biometeorological factors recorded daily: atmospheric pressure, air temperature, degree of insolation, precipitation, relative humidity, water vapour pressure, wind (speed and direction), hydrometeores, index of solar eruption, density of F2 layer. The confrontation is made for the two days before the intoxication and for the day when suicide is attempted. Parameters are then studied by statistical calculation (calculation of X2 and of the number of degrees of freedom). There does not seem to be any significant relationship, in spite of disconcerting series, between most meteorological factors and the number of attempted suicides observed. However, it is noted that no suicides were recorded during periods of solar eruption, and that there seems to be a marked correlation between suicide and winds, particularly according to their direction. Thus, winds charged with ionised particles seem to coincide with a high rate of self-destruction.", "contents": "[Suicidal behavior and meterological conditions]. Six hundred and seventy five cases of attempted suicide observed in a resuscitation department were confronted with a certain number of biometeorological factors recorded daily: atmospheric pressure, air temperature, degree of insolation, precipitation, relative humidity, water vapour pressure, wind (speed and direction), hydrometeores, index of solar eruption, density of F2 layer. The confrontation is made for the two days before the intoxication and for the day when suicide is attempted. Parameters are then studied by statistical calculation (calculation of X2 and of the number of degrees of freedom). There does not seem to be any significant relationship, in spite of disconcerting series, between most meteorological factors and the number of attempted suicides observed. However, it is noted that no suicides were recorded during periods of solar eruption, and that there seems to be a marked correlation between suicide and winds, particularly according to their direction. Thus, winds charged with ionised particles seem to coincide with a high rate of self-destruction.", "PMID": 970821}
{"id": "pubmed23n0032_8198", "title": "[Psychodynamic significance and scoring of \"profile\" and \"silhouette\" responses to Rorschach test in adults and minors].", "content": "\"Profile\" ou \"silhouette\" responses are rarely given at the Rorschach-test (4,87% of our 780 adults and minors; i.e. 7,17% for the adults and 2,56% for the minors). In 82% of our adults and in 50% of our minors, they are indicators of avoiding neurotic behaviour; in 18% of our adults and in 50% of our minors, we are dealing with contact difficulties or insufficiency complexes. These responses are generally unique in each Rorschach test. However 42% of our adults and 30% of our minors gave more than one of them. The VIIth plate holds the first place as a \"stimulus\" for \"profile\" or \"silhouette\" responses (46,22% of the adults and 40% of the minors). The other Rorschach-plates are, in a decreasing order: III, II, I, V and VI for the adults and VII, I, II, and IX for the minors. These responses suggested by the interpretation of the free border of the plates, are De-responses; when human beings are seen as wholes the Rorschach symbol will be G; if only their heads are perceived, it will be: D.", "contents": "[Psychodynamic significance and scoring of \"profile\" and \"silhouette\" responses to Rorschach test in adults and minors]. \"Profile\" ou \"silhouette\" responses are rarely given at the Rorschach-test (4,87% of our 780 adults and minors; i.e. 7,17% for the adults and 2,56% for the minors). In 82% of our adults and in 50% of our minors, they are indicators of avoiding neurotic behaviour; in 18% of our adults and in 50% of our minors, we are dealing with contact difficulties or insufficiency complexes. These responses are generally unique in each Rorschach test. However 42% of our adults and 30% of our minors gave more than one of them. The VIIth plate holds the first place as a \"stimulus\" for \"profile\" or \"silhouette\" responses (46,22% of the adults and 40% of the minors). The other Rorschach-plates are, in a decreasing order: III, II, I, V and VI for the adults and VII, I, II, and IX for the minors. These responses suggested by the interpretation of the free border of the plates, are De-responses; when human beings are seen as wholes the Rorschach symbol will be G; if only their heads are perceived, it will be: D.", "PMID": 970822}
{"id": "pubmed23n0032_8199", "title": "[Psychosomatic reflections].", "content": "This paper resumes the philosophical analysis of the relationship between body and soul. Cartesian dualism is criticized according to the recent developments of the theory of information. The problem of causalities and somato-psychic or psycho-somatic interactions is also criticized. The conclusion aims at a multidisciplinary approach to the patient.", "contents": "[Psychosomatic reflections]. This paper resumes the philosophical analysis of the relationship between body and soul. Cartesian dualism is criticized according to the recent developments of the theory of information. The problem of causalities and somato-psychic or psycho-somatic interactions is also criticized. The conclusion aims at a multidisciplinary approach to the patient.", "PMID": 970823}
{"id": "pubmed23n0032_8200", "title": "[Fa'a'mu and Fanau. Various traditional aspects and current problems of adoption and donation of children in French Polynesia].", "content": "The old polynesian custom of giving a child persits. One child in three or four is asked for and adopted (Fa'a'amu). It was not officially recognized, which had no disadvantage as it was between Maoris and inside enlarged polynesian families. But quick acculturation and urbanization, social and economical changes of the past ten years have changed all that. The contradiction between french law and tahitian traditional customs exposes these fa'a'amu children, with no legal statute and without judicial protection, to many drawbacks. First in their tahitian families because they get no family allowances. On the other hand some Europeans wishing to adopt children take often advantages, though it is illegal, of the generosity of the prolific tahitian women (no contraception in Tahiti) who seldom refuse giving children to a European, without understanding clearly that complete adoption (european model) cuts every tie with the biological family (Fanau). The illegal appropriation of these children sometimes leave them deprived of statute and legal protection in Europe (or America, or even Japan?). We have here an exemple of what happens when two cultures meet, whose customs and values are totally different.", "contents": "[Fa'a'mu and Fanau. Various traditional aspects and current problems of adoption and donation of children in French Polynesia]. The old polynesian custom of giving a child persits. One child in three or four is asked for and adopted (Fa'a'amu). It was not officially recognized, which had no disadvantage as it was between Maoris and inside enlarged polynesian families. But quick acculturation and urbanization, social and economical changes of the past ten years have changed all that. The contradiction between french law and tahitian traditional customs exposes these fa'a'amu children, with no legal statute and without judicial protection, to many drawbacks. First in their tahitian families because they get no family allowances. On the other hand some Europeans wishing to adopt children take often advantages, though it is illegal, of the generosity of the prolific tahitian women (no contraception in Tahiti) who seldom refuse giving children to a European, without understanding clearly that complete adoption (european model) cuts every tie with the biological family (Fanau). The illegal appropriation of these children sometimes leave them deprived of statute and legal protection in Europe (or America, or even Japan?). We have here an exemple of what happens when two cultures meet, whose customs and values are totally different.", "PMID": 970828}
{"id": "pubmed23n0032_8201", "title": "[Late dyskinesia caused by neuroleptics. Survey on 1,660 psychiatric hospital patients].", "content": "Among 1 660 patients of 2 psychiatric hospitals (980 + 680), most of them chronically treated by neuroleptics, we found 120 tardive dyskinesia (7,2%). 64% were more of 50 years old. 85 of 923 women (9,1%), 36 of 737 men (4,8%), but most of the men are younger. In 67 cases oral dyskinesia is isolated, in 53 cases it is associated with choreic syndrom. 13 patients are free of neuroleptics for more than 6 months and the dyskinesia is probably irreversible (mean age = 74). The interest is double: 1. Practical: prevention by moderated and adapted prescription of neuroleptics, specially for long term treatments and oldest patients; anticholinergic correctors must be cautiously prescribed. 2. Theoretical: this human experimentation is a model of iatrogenic pathology for the study of mental illnesses, extrapyramidal troubles, and neuromediators...", "contents": "[Late dyskinesia caused by neuroleptics. Survey on 1,660 psychiatric hospital patients]. Among 1 660 patients of 2 psychiatric hospitals (980 + 680), most of them chronically treated by neuroleptics, we found 120 tardive dyskinesia (7,2%). 64% were more of 50 years old. 85 of 923 women (9,1%), 36 of 737 men (4,8%), but most of the men are younger. In 67 cases oral dyskinesia is isolated, in 53 cases it is associated with choreic syndrom. 13 patients are free of neuroleptics for more than 6 months and the dyskinesia is probably irreversible (mean age = 74). The interest is double: 1. Practical: prevention by moderated and adapted prescription of neuroleptics, specially for long term treatments and oldest patients; anticholinergic correctors must be cautiously prescribed. 2. Theoretical: this human experimentation is a model of iatrogenic pathology for the study of mental illnesses, extrapyramidal troubles, and neuromediators...", "PMID": 970829}
{"id": "pubmed23n0032_8202", "title": "[Short term effects of diets with high levels of dithiocarbamates on carbohydrate and lipid metabolism of rat liver].", "content": "The purpose of the work was to establish the eventual \"metabolic toxicity\" of pesticide-contaminated diets in the Rat. The liver metabolic response to various stimuli was compared in dithiocarbamate-fed animals and in non-contaminated ones. 112 weanling male Wistar CF rats were fed, during 15 days, with a demi-synthetic control diet. They were then divided into 4 lots:--the control group C, which went on to receive the same diet,--the nabame group N, the diet of which was supplemented with 275 ppm of the dithiocarbamate;--the thirame groupe R, receiving the control diet + 600 ppm thirame;--the zineb groupe Z, given the control diet + 3 600 ppm Zineb. The animals were fed with these diets during 14 days, their dithiocarbamate intake thus averaging 1/20 th of the per os LD 50/rat/day. At the end of this 2-week period, each of the 4 groups was divided into 4 sub-groups, all the animals were fasted overnight, then sacrified:--after no other treatment (sub-groups T);--30 minutes after an i.p. injection of 2.6 g/kg glucose (G);--after having been forced to walk in a restraint wheel for 50 minutes/hr during the 18 hrs of the night fast (sub-groups W);--after a 90 minutes exposure in a cold room (F). The weights of the animals, of their liver, heart, kidneys, adrenals and epididymal pads were recorded. In their liver, the following compounds were determined: water, proteins, total lipids, triglycerides, long-chain acyl-CoA, non-esterified fatty acids, total cholesterol, glycogen, glucose, alpha-glycerophosphate. The thirame rats had a lower food intake than the others and the smallest body weight, but their relative liver and kidneys weights were the highest. The nabame animals did not differ from the control ones but the zineb rats had the lightest epididymal fat pads. The primary effects of the dithiocarbamate diets on liver metabolism were apparently not the same in the 3 groups compared to the control ones: nabame and thirame increased glycogen, thirame increased the lipid compounds: long-chain actyl-CoA and triglycerides, where as zineb feeding resulted in an increase of glucose concentration and in a decrease of triglycerides and total lipids. Muscular exercise, or cold exposure, had the following effects compared to those they had in the control group: a greater glucose utilization in the nabame and thirame rats, a smaller glycogen and glucose utilization, associated with an increase of alpha-glycerophosphate, in the zineb animals. These results were considered altogether with those obtained in a previous paper by the same authors, which concerned liver ketone bodies and adenine nucleotides changes after the same experimental conditions, and it was concluded that the 3 dithiocarbamates actually had a common effect on rat metabolism: they all impaired glucose utilization by the liver. Also, fat mobilization from peripheral depots was shown to occur in the 3 experimental groups, resulting in liver fatty acid oxidation in the nabame and zineb rats, and in liver steatosis for the thirame ones...", "contents": "[Short term effects of diets with high levels of dithiocarbamates on carbohydrate and lipid metabolism of rat liver]. The purpose of the work was to establish the eventual \"metabolic toxicity\" of pesticide-contaminated diets in the Rat. The liver metabolic response to various stimuli was compared in dithiocarbamate-fed animals and in non-contaminated ones. 112 weanling male Wistar CF rats were fed, during 15 days, with a demi-synthetic control diet. They were then divided into 4 lots:--the control group C, which went on to receive the same diet,--the nabame group N, the diet of which was supplemented with 275 ppm of the dithiocarbamate;--the thirame groupe R, receiving the control diet + 600 ppm thirame;--the zineb groupe Z, given the control diet + 3 600 ppm Zineb. The animals were fed with these diets during 14 days, their dithiocarbamate intake thus averaging 1/20 th of the per os LD 50/rat/day. At the end of this 2-week period, each of the 4 groups was divided into 4 sub-groups, all the animals were fasted overnight, then sacrified:--after no other treatment (sub-groups T);--30 minutes after an i.p. injection of 2.6 g/kg glucose (G);--after having been forced to walk in a restraint wheel for 50 minutes/hr during the 18 hrs of the night fast (sub-groups W);--after a 90 minutes exposure in a cold room (F). The weights of the animals, of their liver, heart, kidneys, adrenals and epididymal pads were recorded. In their liver, the following compounds were determined: water, proteins, total lipids, triglycerides, long-chain acyl-CoA, non-esterified fatty acids, total cholesterol, glycogen, glucose, alpha-glycerophosphate. The thirame rats had a lower food intake than the others and the smallest body weight, but their relative liver and kidneys weights were the highest. The nabame animals did not differ from the control ones but the zineb rats had the lightest epididymal fat pads. The primary effects of the dithiocarbamate diets on liver metabolism were apparently not the same in the 3 groups compared to the control ones: nabame and thirame increased glycogen, thirame increased the lipid compounds: long-chain actyl-CoA and triglycerides, where as zineb feeding resulted in an increase of glucose concentration and in a decrease of triglycerides and total lipids. Muscular exercise, or cold exposure, had the following effects compared to those they had in the control group: a greater glucose utilization in the nabame and thirame rats, a smaller glycogen and glucose utilization, associated with an increase of alpha-glycerophosphate, in the zineb animals. These results were considered altogether with those obtained in a previous paper by the same authors, which concerned liver ketone bodies and adenine nucleotides changes after the same experimental conditions, and it was concluded that the 3 dithiocarbamates actually had a common effect on rat metabolism: they all impaired glucose utilization by the liver. Also, fat mobilization from peripheral depots was shown to occur in the 3 experimental groups, resulting in liver fatty acid oxidation in the nabame and zineb rats, and in liver steatosis for the thirame ones...", "PMID": 970834}
{"id": "pubmed23n0032_8203", "title": "[Responses and physiological modifications of intestinal sucrase in rats fed diets containing various carbohydrates].", "content": "Weaning rats were submitted during 3 months to complet and balanced diets where only glucidic constituent varied. They were sacrified when fed or after 16 h fasting. 1. The value of intestinal sucrose K(M) is higher in rats fed on glucose dietary than in rats fed on sucrose, fructose or glucose + fructose dietary. These modifications are independant of the states of digestion. 2. The V(MAX) is diversely influenced by the states of digestion: hydric diet increases the V(MAX) in animals fed on sucrose diet when intestinal repletion has the same effect in animals receiving glucose diet. Fructose diet decreases it irreversibility in both cases. 3. Growth of animals is sagging respectively in order from sucrose and glucose diets to glucose + fructose and fructose diets, then weight of organes is unaffected. It was noted that perirenal and epididymal fats are more abundant in rats nourished with glucose diet than with fructose diet. 4. The glucoregulation is correctly effectued in either starved or fed animals and shows an available adaptation of organism in energetic utilisation of various studied sugars. Plasmatic, hepatis and intestinal free fatty acids levels are constant and equal in all series of expements in spite of important variations of reverse fat.", "contents": "[Responses and physiological modifications of intestinal sucrase in rats fed diets containing various carbohydrates]. Weaning rats were submitted during 3 months to complet and balanced diets where only glucidic constituent varied. They were sacrified when fed or after 16 h fasting. 1. The value of intestinal sucrose K(M) is higher in rats fed on glucose dietary than in rats fed on sucrose, fructose or glucose + fructose dietary. These modifications are independant of the states of digestion. 2. The V(MAX) is diversely influenced by the states of digestion: hydric diet increases the V(MAX) in animals fed on sucrose diet when intestinal repletion has the same effect in animals receiving glucose diet. Fructose diet decreases it irreversibility in both cases. 3. Growth of animals is sagging respectively in order from sucrose and glucose diets to glucose + fructose and fructose diets, then weight of organes is unaffected. It was noted that perirenal and epididymal fats are more abundant in rats nourished with glucose diet than with fructose diet. 4. The glucoregulation is correctly effectued in either starved or fed animals and shows an available adaptation of organism in energetic utilisation of various studied sugars. Plasmatic, hepatis and intestinal free fatty acids levels are constant and equal in all series of expements in spite of important variations of reverse fat.", "PMID": 970835}
{"id": "pubmed23n0032_8204", "title": "[Biochemical control of thiamine status in neurotic patients].", "content": "It is well established that the glucose represents the main energy source of the brain and that its metabolism supposes the presence of the vitamin B1. On the other hand, it is known that in industrialized countries, the consume (absolute or relative) of products without vitamin B1 (sugar, strong drinks) or containing it insufficiently (preparations derived from flours of low extraction, canned foods) registered a noticeable growth. On account of these premises, we made the hypothesis that the thiamino-glucidic unbalance to which the modern consumer is exposed, could be a factor contributing to the appearance of neurosis, which present now an increased incidence. In order to verify this hypothesis, we studied the vitamin B1 nutritional status in 65 patients recently taken to hospital with \"nerosis sick\", as well as with 49 healty subjects, who served as controls. We determined the spontaneous thiaminury (concentration, morning hourly debit and per gramme of creatinine), the provoked thiaminury (by means of an loading test, unpublished yet), as well as the erythrocyte transketolase activity and the pyruvic acid in the blood and in the urines (concentration and debit per gramme of creatinine) Compared with the control group, the patients of neurosis the average thiamin excretion was twice reduced, the transketolase activity diminished by 22 per cent, while the pyruvic acid level in blood and its renal elimination were increased by 31-38 per cent (p less than 0,001). After the vitamin B1 treatment, the values changed significatively (p less than 0,05), approaching those found in the healty subjects.", "contents": "[Biochemical control of thiamine status in neurotic patients]. It is well established that the glucose represents the main energy source of the brain and that its metabolism supposes the presence of the vitamin B1. On the other hand, it is known that in industrialized countries, the consume (absolute or relative) of products without vitamin B1 (sugar, strong drinks) or containing it insufficiently (preparations derived from flours of low extraction, canned foods) registered a noticeable growth. On account of these premises, we made the hypothesis that the thiamino-glucidic unbalance to which the modern consumer is exposed, could be a factor contributing to the appearance of neurosis, which present now an increased incidence. In order to verify this hypothesis, we studied the vitamin B1 nutritional status in 65 patients recently taken to hospital with \"nerosis sick\", as well as with 49 healty subjects, who served as controls. We determined the spontaneous thiaminury (concentration, morning hourly debit and per gramme of creatinine), the provoked thiaminury (by means of an loading test, unpublished yet), as well as the erythrocyte transketolase activity and the pyruvic acid in the blood and in the urines (concentration and debit per gramme of creatinine) Compared with the control group, the patients of neurosis the average thiamin excretion was twice reduced, the transketolase activity diminished by 22 per cent, while the pyruvic acid level in blood and its renal elimination were increased by 31-38 per cent (p less than 0,001). After the vitamin B1 treatment, the values changed significatively (p less than 0,05), approaching those found in the healty subjects.", "PMID": 970836}
{"id": "pubmed23n0032_8205", "title": "Polynucleotide sequence divergence among strains of Salmonella sub-genus IV and closely related organisms.", "content": "Polynucleotide sequence relatedness studies were carried out to determine the extent of divergence present in Salmonella sub-genus IV strains, related strains of Salmonella of other sub-genera and the Citrobacter genus. Salmonella sub-genus IV were 91-97% related. The Salmonella of sub-genus I, II and III showed lower binding (79-87%) to Salmonella sub-genus IV. The change in thermal elution midpoint closely followed the reassociation data. Relatedness of Salmonella sub-genus IV and C. freundii ranged between 44 and 57%, which confirms that these organisms belong to different genera. The taxonomic position of Salmonella sub-genus IV is discussed according to the actual classification of the family Enterobacteriaceae.", "contents": "Polynucleotide sequence divergence among strains of Salmonella sub-genus IV and closely related organisms. Polynucleotide sequence relatedness studies were carried out to determine the extent of divergence present in Salmonella sub-genus IV strains, related strains of Salmonella of other sub-genera and the Citrobacter genus. Salmonella sub-genus IV were 91-97% related. The Salmonella of sub-genus I, II and III showed lower binding (79-87%) to Salmonella sub-genus IV. The change in thermal elution midpoint closely followed the reassociation data. Relatedness of Salmonella sub-genus IV and C. freundii ranged between 44 and 57%, which confirms that these organisms belong to different genera. The taxonomic position of Salmonella sub-genus IV is discussed according to the actual classification of the family Enterobacteriaceae.", "PMID": 970830}
{"id": "pubmed23n0032_8206", "title": "[Experimental infection of axenic mice by \"Yersinia enterocolitica\" (author's transl)].", "content": "Although most of Yersinia enterocolitica strains isolated from man have no pathogenicity for laboratory animals, it has been demonstrated that some strains are pathogenic for conventional mice and that most of the strains are probably pathogenic for Nude mice. The authors report the results of the infection of germ-free mice with a strain of Y. enterocolitica which is non pathogenic for holoxenic mice. It appears that C3H/He mice are sensitive to the infection by gavage or aerogenic and peritoneal routes. They all die within 8 to 12 days after injection of an inoculum of 5.10(5) viable cells. Germ-free NCS mice were also sensitive to the oral and aerogenic infection but not to the peritoneal infection; the difference between C3H/He and NCS sensitivity to this way of infection could be explained by a higher bactericidal activity of the peritoneal phagocytes of the latter. The C3H/He and NCS holoxenic control mice infected with the same inoculum of the same strain, did not show any symptoms and all attempts to isolate Y. enterocolitica failed three months after the challenge. Germ-free mice killed by the infection showed histopathological findings, i.e. abscesses involving intestinal wall. liver and spleen; they were similar to those described in experiments with pathogenic strains for conventional mice (holoxenic) and to those observed in infection of athymic Nude mice with strains non pathogenic for conventional mice. This infectious disease model is discussed in regards to the natural human infection.", "contents": "[Experimental infection of axenic mice by \"Yersinia enterocolitica\" (author's transl)]. Although most of Yersinia enterocolitica strains isolated from man have no pathogenicity for laboratory animals, it has been demonstrated that some strains are pathogenic for conventional mice and that most of the strains are probably pathogenic for Nude mice. The authors report the results of the infection of germ-free mice with a strain of Y. enterocolitica which is non pathogenic for holoxenic mice. It appears that C3H/He mice are sensitive to the infection by gavage or aerogenic and peritoneal routes. They all die within 8 to 12 days after injection of an inoculum of 5.10(5) viable cells. Germ-free NCS mice were also sensitive to the oral and aerogenic infection but not to the peritoneal infection; the difference between C3H/He and NCS sensitivity to this way of infection could be explained by a higher bactericidal activity of the peritoneal phagocytes of the latter. The C3H/He and NCS holoxenic control mice infected with the same inoculum of the same strain, did not show any symptoms and all attempts to isolate Y. enterocolitica failed three months after the challenge. Germ-free mice killed by the infection showed histopathological findings, i.e. abscesses involving intestinal wall. liver and spleen; they were similar to those described in experiments with pathogenic strains for conventional mice (holoxenic) and to those observed in infection of athymic Nude mice with strains non pathogenic for conventional mice. This infectious disease model is discussed in regards to the natural human infection.", "PMID": 970831}
{"id": "pubmed23n0032_8207", "title": "[Calcium absorption by the rat from various milks in relation to their total phosphorus and lactose contents].", "content": "The absorption of calcium corresponds to a strictly determined mechanism inhibited by phosphates and activited by carbohydrates. We investigate in what extent the absorption of milk calcium from various species has the same proceeding. 4 months old rats are given orally solutions of CaCl2 alone or in combination with sodium dihydrogen phosphate or lactose or these both compounds. We compare calcium absorption of these solutions to that of milk from woman, cow or sow, or to dilutions of these two latter milks. All these fluids are dosed so that they correspond each other by their respective content in total calcium, total phosphorus and lactose. Each solution contains 45Ca. Blood samples 1,30, 4, 6 and 24 hours after ingestion allow establishing the variations of plasma radioactivity. Rats are sacrified after 24 hours. In certain cases, samples from digestive tube contents and feces provide a coefficient of calcium absorption. The osseous retention is obtained from femur radioactivity. In breif, we may consider that milks from cow and sow provide calcium the absorption of which is settled after their respective content of total phosphorus and lactose. Opposed effects of these inhibitors and activators of calcium absorption compensate so that calcium from these milks is just a little better utilized by adult rat than calcium from an isocalcic solution of CaCl2. Calcium from woman milk, rich in carbohydrates, poor in total phosphorus is better absorbed than that from the two latters, however less than we might expect from its high lactose content. We may wonder that calcium utilization from cow milk is as moderate as that of a solution of CaCl2. But skeleton mineralization which may be fulfilled by milk is made better because of that: it is not chloride but calcium phosphate which ensures this mineralization, a phosphate which alone would impair this mineralization without the compensative role of lactose.", "contents": "[Calcium absorption by the rat from various milks in relation to their total phosphorus and lactose contents]. The absorption of calcium corresponds to a strictly determined mechanism inhibited by phosphates and activited by carbohydrates. We investigate in what extent the absorption of milk calcium from various species has the same proceeding. 4 months old rats are given orally solutions of CaCl2 alone or in combination with sodium dihydrogen phosphate or lactose or these both compounds. We compare calcium absorption of these solutions to that of milk from woman, cow or sow, or to dilutions of these two latter milks. All these fluids are dosed so that they correspond each other by their respective content in total calcium, total phosphorus and lactose. Each solution contains 45Ca. Blood samples 1,30, 4, 6 and 24 hours after ingestion allow establishing the variations of plasma radioactivity. Rats are sacrified after 24 hours. In certain cases, samples from digestive tube contents and feces provide a coefficient of calcium absorption. The osseous retention is obtained from femur radioactivity. In breif, we may consider that milks from cow and sow provide calcium the absorption of which is settled after their respective content of total phosphorus and lactose. Opposed effects of these inhibitors and activators of calcium absorption compensate so that calcium from these milks is just a little better utilized by adult rat than calcium from an isocalcic solution of CaCl2. Calcium from woman milk, rich in carbohydrates, poor in total phosphorus is better absorbed than that from the two latters, however less than we might expect from its high lactose content. We may wonder that calcium utilization from cow milk is as moderate as that of a solution of CaCl2. But skeleton mineralization which may be fulfilled by milk is made better because of that: it is not chloride but calcium phosphate which ensures this mineralization, a phosphate which alone would impair this mineralization without the compensative role of lactose.", "PMID": 970837}
{"id": "pubmed23n0032_8208", "title": "[Changes in weight of fresh and dried vegetables on cooking].", "content": "The present study represents part of a more important work relative to normal food refuse and change of weight of varied food according to different ways of cooking. The study has been realized on vegetables and some fruits of frequent utilization in the population. It was accomplished by 25 voluntary housekeepers from Paris and Marseille during a whole year according to precise instructions about the method of weighings, every vegetable having to be weighed, three times at different intervals, during the year; 3203 weighings were mode corresponding to 2 399 kg of divers types of vegetables. The results have pointed out important variations according to the kinds of vegetables and methods of cooking. At boiling, the most utilized method, the variations of weight according to the weight before cooking are very important extending from + 10 p. 100, for Brussel sprouts and fresh flageolets at--25 p. 100 and--36 p. 100 for lettuce and endive, the last of weight being the highest for fine leaves vegetables, lesser for roots and tubers, and around zero for artichokes, french beans, cauliflower, aubergines. Other methods of cooking, through concerning a more limited number of kinds of vegetables have pointed out that \"saut\u00e9\" brings a higher loss for the vegetables studied; Similar observations can be made for braising and stewing, frying leads to the highest loss reaching--48 p. 100 for chips. However the weight of fresh pulses varies slightly at boiling and stewing, it is the same for potatoes. As for dry beans, the increase of weight is less important than the percentage curently utilized for the calculation of surveys, the loss of weight according to cooked fruits pears and apples is not high (about 10 p. 100). These results should be taken into consideration for the exploitation of food surveys, especially norvadays when surveys are made more often at home and at the hospital and concern the individual consumption either by weighing the food consumed at table in dining-room or on the tray of the patient, and also when surveys are realized by interrogatory, the food being then, cooked, without refuse. From this point of view the last two tables present the results for a practical utilization. The first one gives the different kinds of vegetables studied with every way of cooking observed, the numbers of weighings effected the weight corresponding to 100 g raw and the weight corresponding to 100 g cooked, the other one presents the kinds of vegetables classified according to importance of the variations of weight for different ways of cooking in connection with the weight before cooking.", "contents": "[Changes in weight of fresh and dried vegetables on cooking]. The present study represents part of a more important work relative to normal food refuse and change of weight of varied food according to different ways of cooking. The study has been realized on vegetables and some fruits of frequent utilization in the population. It was accomplished by 25 voluntary housekeepers from Paris and Marseille during a whole year according to precise instructions about the method of weighings, every vegetable having to be weighed, three times at different intervals, during the year; 3203 weighings were mode corresponding to 2 399 kg of divers types of vegetables. The results have pointed out important variations according to the kinds of vegetables and methods of cooking. At boiling, the most utilized method, the variations of weight according to the weight before cooking are very important extending from + 10 p. 100, for Brussel sprouts and fresh flageolets at--25 p. 100 and--36 p. 100 for lettuce and endive, the last of weight being the highest for fine leaves vegetables, lesser for roots and tubers, and around zero for artichokes, french beans, cauliflower, aubergines. Other methods of cooking, through concerning a more limited number of kinds of vegetables have pointed out that \"saut\u00e9\" brings a higher loss for the vegetables studied; Similar observations can be made for braising and stewing, frying leads to the highest loss reaching--48 p. 100 for chips. However the weight of fresh pulses varies slightly at boiling and stewing, it is the same for potatoes. As for dry beans, the increase of weight is less important than the percentage curently utilized for the calculation of surveys, the loss of weight according to cooked fruits pears and apples is not high (about 10 p. 100). These results should be taken into consideration for the exploitation of food surveys, especially norvadays when surveys are made more often at home and at the hospital and concern the individual consumption either by weighing the food consumed at table in dining-room or on the tray of the patient, and also when surveys are realized by interrogatory, the food being then, cooked, without refuse. From this point of view the last two tables present the results for a practical utilization. The first one gives the different kinds of vegetables studied with every way of cooking observed, the numbers of weighings effected the weight corresponding to 100 g raw and the weight corresponding to 100 g cooked, the other one presents the kinds of vegetables classified according to importance of the variations of weight for different ways of cooking in connection with the weight before cooking.", "PMID": 970838}
{"id": "pubmed23n0032_8209", "title": "[Dwarf colony mutants of \"staphylococcus\": study of three strains isolated from patients with osteosynthesis (author's transl)].", "content": "Dwarf colony mutants of staphylococci were isolated from per operative samplings obtained from three patients with a bacterial contamination of their osteosynthesis material. The dwarf mutants grown in trypticase soja agar (TSA) after 72 h at 37 degrees C (two cases) and in nutrient broth (one case). They displayed poor growth (very small, transparent colonies on TSA), reduced biochemical activity, deficient pigment formation and intermediary resistance to aminoglucoside antibiotics (kanamycin, streptomycin, gentamicin). Biochemically, two types of metabolic defects have been demonstrated among these dwarf strains. Two of them are vitamin K2-less (strains 30 and 73) and are supplemented by menadion natrium bisulfit at concentrations ranging from 0.2 to 10 mug per ml. In these cases the dwarf colony mutants coexisted with normal colony of S. epidermidis (case 30) and S. aureus (case 73) belonging to serotypes 52260 and I respectively. The third strain (case 163) supplemented by horse blood is hemin-requiring. This strain could be characterized in media supplemented with blood, because it was isolated only as dwarf colony mutant from the patient; it belongs to serotype 52260 (S. epidermidis). This hemin-less dwarf mutant is the single responsible of the pathological manifestations of the patient 163. In the cases of vitamin K12-less mutants (strains 30 and 73) the coexistence of dwarf mutants and normal colonies of the same serotype may make possible the elucidation of relapse in infectious complications of the patients with osteosynthesis.", "contents": "[Dwarf colony mutants of \"staphylococcus\": study of three strains isolated from patients with osteosynthesis (author's transl)]. Dwarf colony mutants of staphylococci were isolated from per operative samplings obtained from three patients with a bacterial contamination of their osteosynthesis material. The dwarf mutants grown in trypticase soja agar (TSA) after 72 h at 37 degrees C (two cases) and in nutrient broth (one case). They displayed poor growth (very small, transparent colonies on TSA), reduced biochemical activity, deficient pigment formation and intermediary resistance to aminoglucoside antibiotics (kanamycin, streptomycin, gentamicin). Biochemically, two types of metabolic defects have been demonstrated among these dwarf strains. Two of them are vitamin K2-less (strains 30 and 73) and are supplemented by menadion natrium bisulfit at concentrations ranging from 0.2 to 10 mug per ml. In these cases the dwarf colony mutants coexisted with normal colony of S. epidermidis (case 30) and S. aureus (case 73) belonging to serotypes 52260 and I respectively. The third strain (case 163) supplemented by horse blood is hemin-requiring. This strain could be characterized in media supplemented with blood, because it was isolated only as dwarf colony mutant from the patient; it belongs to serotype 52260 (S. epidermidis). This hemin-less dwarf mutant is the single responsible of the pathological manifestations of the patient 163. In the cases of vitamin K12-less mutants (strains 30 and 73) the coexistence of dwarf mutants and normal colonies of the same serotype may make possible the elucidation of relapse in infectious complications of the patients with osteosynthesis.", "PMID": 970832}
{"id": "pubmed23n0032_8210", "title": "[Study of Enterobacter strains belonging to a particular group related to Enterobacter aerogenes (author's transl)].", "content": "Thirty strains of Enterobacteriaceae constitute a new group related to E. aerogenes with the following differential biochemical characters: urease+, KCN-, sorbitol-, mucate- and gelatinase-. Ten of these strains with antigenic similarities and polyresistance to antibiotics were isolated from urine in the same hospital.", "contents": "[Study of Enterobacter strains belonging to a particular group related to Enterobacter aerogenes (author's transl)]. Thirty strains of Enterobacteriaceae constitute a new group related to E. aerogenes with the following differential biochemical characters: urease+, KCN-, sorbitol-, mucate- and gelatinase-. Ten of these strains with antigenic similarities and polyresistance to antibiotics were isolated from urine in the same hospital.", "PMID": 970833}
{"id": "pubmed23n0032_8211", "title": "[Influence of some nutritional factors on the canthaxanthine pigmentation of the rainbow trout].", "content": "The present investigation was made to approve nutritionnal factors which affect the utilisation of food canthaxanthin by hatchery trout. Rainbow trout were obtained from a commercial trout farm, kept in running water and feeding in experimental pellets for 4 to 8 weeks. Five diets were tested (1-standard [7.5 u.i/g], 2-low protein content, 3-High lipid content, 4-non supplemented with vitamin A, 5-High vitamin A content [23 U.I./g]). All diets containing 250 mg of canthaxanthin per kilo of food, the fish were fed at the rate of approximately 2.5 p. 100 of their body weight per day. The estimation of canthaxanthin from fish tissues was accomplished as follows: the tissues were gomogeneized and extracted with several volumes of solvents. The solution containing all the pigment was evaporated. The purification of the canthaxanthin was obtained by column silica gel chromatography. The pigment was eluated with ethyl-ether as solvent. Quantitative assay of total caroteno\u00efd was effected by spectre photometric estimation of optical density at 480 nm of this fraction dissolved in benzene. The caroteno\u00efd composition of this fraction was studied by silica-gel thin layer chromatography. In these conditions:--it was shown by spectral studies that the canthaxanthins present in the diet are essentially those deposed in the flesh of the trout fed;--a series of experiment were made to examine the effect of composition of artificially composed feeds for pigmentation of the rainbow trout. The pigment deposition was found to correlate with the amount of lipids present in these diet. In this condition, the pigment is more dispersed with lipids present and the pigment absorption may be facilitated and later be deposed. 8 weeks alimentation are needed to observe the effect of these nutritionnal factor;--when the diet is non supplemented with vitamin A, the same quantity of canthaxanthin mixed with this food gives trout redder flesh than the standard diet. On an other hand, an antagonist effect of diet with high vitamin A content is demonstrated on the amount of the pigment deposed in the flesh of trout;--no effect of protein content of the diet was found in these experiments;--no effect of ratio calorie/protein of the diets with the intensity of deposed pigment in the flesh rainbow trout;--a correlation between neutral lipids of the tissue and the pigment deposed in the flesh of the trout fed with canthaxanthin was confirmed.", "contents": "[Influence of some nutritional factors on the canthaxanthine pigmentation of the rainbow trout]. The present investigation was made to approve nutritionnal factors which affect the utilisation of food canthaxanthin by hatchery trout. Rainbow trout were obtained from a commercial trout farm, kept in running water and feeding in experimental pellets for 4 to 8 weeks. Five diets were tested (1-standard [7.5 u.i/g], 2-low protein content, 3-High lipid content, 4-non supplemented with vitamin A, 5-High vitamin A content [23 U.I./g]). All diets containing 250 mg of canthaxanthin per kilo of food, the fish were fed at the rate of approximately 2.5 p. 100 of their body weight per day. The estimation of canthaxanthin from fish tissues was accomplished as follows: the tissues were gomogeneized and extracted with several volumes of solvents. The solution containing all the pigment was evaporated. The purification of the canthaxanthin was obtained by column silica gel chromatography. The pigment was eluated with ethyl-ether as solvent. Quantitative assay of total caroteno\u00efd was effected by spectre photometric estimation of optical density at 480 nm of this fraction dissolved in benzene. The caroteno\u00efd composition of this fraction was studied by silica-gel thin layer chromatography. In these conditions:--it was shown by spectral studies that the canthaxanthins present in the diet are essentially those deposed in the flesh of the trout fed;--a series of experiment were made to examine the effect of composition of artificially composed feeds for pigmentation of the rainbow trout. The pigment deposition was found to correlate with the amount of lipids present in these diet. In this condition, the pigment is more dispersed with lipids present and the pigment absorption may be facilitated and later be deposed. 8 weeks alimentation are needed to observe the effect of these nutritionnal factor;--when the diet is non supplemented with vitamin A, the same quantity of canthaxanthin mixed with this food gives trout redder flesh than the standard diet. On an other hand, an antagonist effect of diet with high vitamin A content is demonstrated on the amount of the pigment deposed in the flesh of trout;--no effect of protein content of the diet was found in these experiments;--no effect of ratio calorie/protein of the diets with the intensity of deposed pigment in the flesh rainbow trout;--a correlation between neutral lipids of the tissue and the pigment deposed in the flesh of the trout fed with canthaxanthin was confirmed.", "PMID": 970839}
{"id": "pubmed23n0032_8212", "title": "Effect of pilocarpine ocular therapeutic systems on diurnal control of intraocular pressure.", "content": "The pilocarpine ocular therapeutic system is an innovative ocular drug delivery system which releases pilocarpine into the tear film at a constant rate of 20mug/hr or 40mug/hr. The system was evaluated in 22 hospitalized patients with bilateral open angle glaucoma to determine its ability to control ocular pressure throughout the diurnal interval. Only the right eye of each patient was treated with the pilocarpine ocular therapeutic system; the other eye served as a control. Twelve pressure measurements were made with a noncontacttorometer over a 34-hour test period. In general, the pilocarpine ocular therapeutic system provided satisfactory around-the-clock control of intraocular pressure and was well tolerated by the patients.", "contents": "Effect of pilocarpine ocular therapeutic systems on diurnal control of intraocular pressure. The pilocarpine ocular therapeutic system is an innovative ocular drug delivery system which releases pilocarpine into the tear film at a constant rate of 20mug/hr or 40mug/hr. The system was evaluated in 22 hospitalized patients with bilateral open angle glaucoma to determine its ability to control ocular pressure throughout the diurnal interval. Only the right eye of each patient was treated with the pilocarpine ocular therapeutic system; the other eye served as a control. Twelve pressure measurements were made with a noncontacttorometer over a 34-hour test period. In general, the pilocarpine ocular therapeutic system provided satisfactory around-the-clock control of intraocular pressure and was well tolerated by the patients.", "PMID": 970853}
{"id": "pubmed23n0032_8213", "title": "Chromophobe adenoma and chiasmal syndrome without enlargement of the bony sella.", "content": "Four cases of chiasmal syndrome resulting from chromophobe adenoma with normal-sized sella turcica are reported. The predominantly suprasellar growth of these tumors was probably the result of a well-pneumatized sphenoid sinus and/or a congenitally deficient diaphragma sellae. In patients with chiasmal syndrome, a high suspicion of a surgically treatable lesion must be maintained in spite of radiographs showing a normal-sized sella. In spite of the difficulties presented by normal variations, subtle findings should be searched for on the plain films and given additional weight in the patient with a chiasmal syndrome. An aggressive diagnostic work-up, including pneumoencephalography with thin-section tomography, should be pursued in all such patients before accepting some alternate explanation, such as demyelinating disease, for visual impairment.", "contents": "Chromophobe adenoma and chiasmal syndrome without enlargement of the bony sella. Four cases of chiasmal syndrome resulting from chromophobe adenoma with normal-sized sella turcica are reported. The predominantly suprasellar growth of these tumors was probably the result of a well-pneumatized sphenoid sinus and/or a congenitally deficient diaphragma sellae. In patients with chiasmal syndrome, a high suspicion of a surgically treatable lesion must be maintained in spite of radiographs showing a normal-sized sella. In spite of the difficulties presented by normal variations, subtle findings should be searched for on the plain films and given additional weight in the patient with a chiasmal syndrome. An aggressive diagnostic work-up, including pneumoencephalography with thin-section tomography, should be pursued in all such patients before accepting some alternate explanation, such as demyelinating disease, for visual impairment.", "PMID": 970854}
{"id": "pubmed23n0032_8214", "title": "Oribital floor fractures: a retrospective study.", "content": "A retrospective series of 365 orbital floor fractures was studied and many variables were analyzed. Some type of ocular or orbital adnexal damage was present in 32% of the patients. After a repair procedure there was a low incidence of decreased visual acuity, and there were no implant extrusions. Late enophthalmost and diplopia were found to be related to the type of orbital floor damage. The comminuted floor fractures and fractures associated with prolapse of orbital tissue into the maxillary antrum were more prone to demonstrate these late sequelae. Despite various surgical procedures there was a 19% incidence of late diplopia and an 11% incidence of late enophthalmos.", "contents": "Oribital floor fractures: a retrospective study. A retrospective series of 365 orbital floor fractures was studied and many variables were analyzed. Some type of ocular or orbital adnexal damage was present in 32% of the patients. After a repair procedure there was a low incidence of decreased visual acuity, and there were no implant extrusions. Late enophthalmost and diplopia were found to be related to the type of orbital floor damage. The comminuted floor fractures and fractures associated with prolapse of orbital tissue into the maxillary antrum were more prone to demonstrate these late sequelae. Despite various surgical procedures there was a 19% incidence of late diplopia and an 11% incidence of late enophthalmos.", "PMID": 970855}
{"id": "pubmed23n0032_8215", "title": "Specific retinal breaks in aphakic retinal separation.", "content": "The frequency and location of specific retinal breaks in a consecutive series of 169 phakic and 121 aphakic primary nontraumatic rhegmatogenous retinal separations categorized into lattice and nonlattice groups was determined. Small horseshoe tears in the oral region of the retina at the posterior vitreous base were responsible for 44.1% of aphakic nonlattice detechments. Aphakic detachments associated with lattice degeneration had a greater relative frequency of associated nonlattice tractional breaks in addition to lattice breaks.", "contents": "Specific retinal breaks in aphakic retinal separation. The frequency and location of specific retinal breaks in a consecutive series of 169 phakic and 121 aphakic primary nontraumatic rhegmatogenous retinal separations categorized into lattice and nonlattice groups was determined. Small horseshoe tears in the oral region of the retina at the posterior vitreous base were responsible for 44.1% of aphakic nonlattice detechments. Aphakic detachments associated with lattice degeneration had a greater relative frequency of associated nonlattice tractional breaks in addition to lattice breaks.", "PMID": 970857}
{"id": "pubmed23n0032_8216", "title": "The effect of light deprivation on the flickering B-wave.", "content": "The effect of light deprivation on the flickering beta-wave was investagated. Results indicate that the flickering beta-wave is extremely reduced in the eye light-deprived for 14 to 20 days relative to the normal eye. The ratio of the second beta-wave (in a series of flickering beta-waves) to the ratio of the first beta-wave appears relatively unaffected by light deprivation, but the ratio of the fourth beta-wave to the first is significantly affected by light deprivation. The effect of light deprivation on the beta-waves evoked by a 13.4 milliambert flickering light is greater for beta-waves evoked later in the series than for beta-waves evoked earlier in the series.", "contents": "The effect of light deprivation on the flickering B-wave. The effect of light deprivation on the flickering beta-wave was investagated. Results indicate that the flickering beta-wave is extremely reduced in the eye light-deprived for 14 to 20 days relative to the normal eye. The ratio of the second beta-wave (in a series of flickering beta-waves) to the ratio of the first beta-wave appears relatively unaffected by light deprivation, but the ratio of the fourth beta-wave to the first is significantly affected by light deprivation. The effect of light deprivation on the beta-waves evoked by a 13.4 milliambert flickering light is greater for beta-waves evoked later in the series than for beta-waves evoked earlier in the series.", "PMID": 970858}
{"id": "pubmed23n0032_8217", "title": "Serum factors precipitating with bovine lens extract in cataract patients and noncataract patients.", "content": "The present study tested the assertion that there exists exclusively in persons with senile cataracts a serum factor capable of precipitating with gamma crystallin extract from bovine lens. The results of this work do not support this assertion without the imposition of rigid specifications regarding test solution concentrations and observation time. Among the 84 subjects tested, 2 individuals had posterior subcapsular cataracts. The precipitates resulting from incubation with serum from these individuals were similar to those resulting from incubations with serum from senile cataract patients. The present study did not confirm the existence of exclusive serum factors capable of precipitating with gamma crystallin extract in patients with senile cataracts.", "contents": "Serum factors precipitating with bovine lens extract in cataract patients and noncataract patients. The present study tested the assertion that there exists exclusively in persons with senile cataracts a serum factor capable of precipitating with gamma crystallin extract from bovine lens. The results of this work do not support this assertion without the imposition of rigid specifications regarding test solution concentrations and observation time. Among the 84 subjects tested, 2 individuals had posterior subcapsular cataracts. The precipitates resulting from incubation with serum from these individuals were similar to those resulting from incubations with serum from senile cataract patients. The present study did not confirm the existence of exclusive serum factors capable of precipitating with gamma crystallin extract in patients with senile cataracts.", "PMID": 970859}
{"id": "pubmed23n0032_8218", "title": "Studies in diagnosis of choroidal melanoma: use of 67Gallium.", "content": "A series of clinically suspected malignant melanomas was used to test the usefulness of 67Gallium citrate using external counting techniques. The comparison was made with angiograms, 32P uptake, and clinical examinations. 67Gallium citrate uptkae proved to be an unreliable diagnostic tool with the described techniques. However, sufficient tissue concentration was obtained tin the tumor to suggest that improved probe counting systems with better resolution of ocular from extraocular tissue may allow this to become a useful technique in differentiating tumor from nontumor ocular pathology. A good indirect and direct ophthalmoscopic examination, along with periodic observation, were the most useful techniques for diagnosis in these cases. 32P uptake was extremely reliable (100%) and quite helpful in assuring the proper diagnosis.", "contents": "Studies in diagnosis of choroidal melanoma: use of 67Gallium. A series of clinically suspected malignant melanomas was used to test the usefulness of 67Gallium citrate using external counting techniques. The comparison was made with angiograms, 32P uptake, and clinical examinations. 67Gallium citrate uptkae proved to be an unreliable diagnostic tool with the described techniques. However, sufficient tissue concentration was obtained tin the tumor to suggest that improved probe counting systems with better resolution of ocular from extraocular tissue may allow this to become a useful technique in differentiating tumor from nontumor ocular pathology. A good indirect and direct ophthalmoscopic examination, along with periodic observation, were the most useful techniques for diagnosis in these cases. 32P uptake was extremely reliable (100%) and quite helpful in assuring the proper diagnosis.", "PMID": 970860}
{"id": "pubmed23n0032_8219", "title": "[Rendu-Osler disease: apropos of 50 cases followed at the Gustave-Roussy Institute].", "content": "Rendu-Osler disease is an hereditary disease due to a predominantly antosomic heredity. The statistics given for 50 cases include 27 males 7 p. 100 of which had no familial antecedents. Clinically, Rendu-Osler disease begins in adulthood (44 p. 100) and often in late adulthood. In fact, it does not become really severe until about the age of 50 or 60. Epistaxis is the most frequent accident and proves fatal in more than 10 p. 100 of cases. Treatment of the nasal symptoms, whether this involves surgical excision followed by grafting or plesiocurietherapy using irridium wires, is very disappointing. The future alone will prove whether embolization gives more permanent results.", "contents": "[Rendu-Osler disease: apropos of 50 cases followed at the Gustave-Roussy Institute]. Rendu-Osler disease is an hereditary disease due to a predominantly antosomic heredity. The statistics given for 50 cases include 27 males 7 p. 100 of which had no familial antecedents. Clinically, Rendu-Osler disease begins in adulthood (44 p. 100) and often in late adulthood. In fact, it does not become really severe until about the age of 50 or 60. Epistaxis is the most frequent accident and proves fatal in more than 10 p. 100 of cases. Treatment of the nasal symptoms, whether this involves surgical excision followed by grafting or plesiocurietherapy using irridium wires, is very disappointing. The future alone will prove whether embolization gives more permanent results.", "PMID": 970861}
{"id": "pubmed23n0032_8220", "title": "[Super-selective arteriography and embolization in severe epistaxis].", "content": "Super-selective arteriography technique is described. This examination enables the finest collaterals of the branches of the external carotid to be shown. Numerous anastomoses which explain the ineffectiveness of certain arterial ligatures used in epistaxis can be seen. Embolization has become an extremely common method for all facial malformations responsible for epistaxis and for O.-R.-L. tumours fed by the external carotid. Several cases are described which demonstrate that embolization in severe epistaxis is a speedy, elegant and permanent method.", "contents": "[Super-selective arteriography and embolization in severe epistaxis]. Super-selective arteriography technique is described. This examination enables the finest collaterals of the branches of the external carotid to be shown. Numerous anastomoses which explain the ineffectiveness of certain arterial ligatures used in epistaxis can be seen. Embolization has become an extremely common method for all facial malformations responsible for epistaxis and for O.-R.-L. tumours fed by the external carotid. Several cases are described which demonstrate that embolization in severe epistaxis is a speedy, elegant and permanent method.", "PMID": 970862}
{"id": "pubmed23n0032_8221", "title": "[Trichocephaloidis beauporti n. sp., new cestode of Charadriiformes and some Passeriformes in Guadelupa (author's transl)].", "content": "Description of a new species of Dilepididae, Trichocephaloidis beauporti n. sp., parasite of Charadriiform Birds (Tringa flaviceps; Micropalama himantopus; Gallinago gallinago delicata; Squatarola squatarola) of Guadelupa and also of a Passeriforme, Quiscalus lugubris. This species can easily be separated from other Trichocephaloidis by the structure of bifid rostellum and the length of Hooks (70-77 mu). Trichocephaloidis charadrii is synonym of Trichocephaloidis megalocephala.", "contents": "[Trichocephaloidis beauporti n. sp., new cestode of Charadriiformes and some Passeriformes in Guadelupa (author's transl)]. Description of a new species of Dilepididae, Trichocephaloidis beauporti n. sp., parasite of Charadriiform Birds (Tringa flaviceps; Micropalama himantopus; Gallinago gallinago delicata; Squatarola squatarola) of Guadelupa and also of a Passeriforme, Quiscalus lugubris. This species can easily be separated from other Trichocephaloidis by the structure of bifid rostellum and the length of Hooks (70-77 mu). Trichocephaloidis charadrii is synonym of Trichocephaloidis megalocephala.", "PMID": 970865}
{"id": "pubmed23n0032_8222", "title": "[Our attitude toward so-called essential severe epistaxis. The problem of vascular ligatures].", "content": "The authors describe their therapeutic attitude over the past few years towards severe epistaxis. In an emergency, anterior and posterior tamponage using thick tents is the main treatment used. Double balloon probes have been discontinued, in spite of the fact that they were well-tolerated, on account of the disappointing results they gave. In cases of relapse, when the posterior tampon is removed after 48 hours, systematic ligature of the sphenopalatine artery is carried out on arteriosclerosis patients aged about fifty who have high blood pressure. In other cases, the authors wait to see the effect of leaving a second tampon in place for a further 48 hours. In patients suffering from renal inadequacy, reabsorbable tents are preferable, prevention being the main aim, avoiding all treatment liable to counteract haemostasis. Generally speaking, ligature of the external carotid is often ineffective. It is the sphenopalatine artery which should be tied off (100 cases in the O.-R.L. Dept. of the H\u00f4pital Foch). Ligature of the ethmoid is rarely indicated.", "contents": "[Our attitude toward so-called essential severe epistaxis. The problem of vascular ligatures]. The authors describe their therapeutic attitude over the past few years towards severe epistaxis. In an emergency, anterior and posterior tamponage using thick tents is the main treatment used. Double balloon probes have been discontinued, in spite of the fact that they were well-tolerated, on account of the disappointing results they gave. In cases of relapse, when the posterior tampon is removed after 48 hours, systematic ligature of the sphenopalatine artery is carried out on arteriosclerosis patients aged about fifty who have high blood pressure. In other cases, the authors wait to see the effect of leaving a second tampon in place for a further 48 hours. In patients suffering from renal inadequacy, reabsorbable tents are preferable, prevention being the main aim, avoiding all treatment liable to counteract haemostasis. Generally speaking, ligature of the external carotid is often ineffective. It is the sphenopalatine artery which should be tied off (100 cases in the O.-R.L. Dept. of the H\u00f4pital Foch). Ligature of the ethmoid is rarely indicated.", "PMID": 970863}
{"id": "pubmed23n0032_8223", "title": "[Rendu-Osler disease treated by endonasal radiotherapy (evaluation of 62 cases)].", "content": "Two groups of patients were treated by plesiocurietherapy. The first group of 17 patients received only one exposure to radium (between 1949 and 1960). The second group received one or more doses of irridium 192 (between 1960 and 1973). The average period for stabilization of the disease was 13,2 months with radium and 20,4 months with irridium. Unfortunately, this does not give a permanent cure and, for irridium 192, the percentage of successes is only 30 p. 100 after 5 years. Logically, radiotherapy with irridium 192 should only be used when other methods have failed. The progressive accumulation of radio-lesions may, in fact, make the use of other techniques impossible.", "contents": "[Rendu-Osler disease treated by endonasal radiotherapy (evaluation of 62 cases)]. Two groups of patients were treated by plesiocurietherapy. The first group of 17 patients received only one exposure to radium (between 1949 and 1960). The second group received one or more doses of irridium 192 (between 1960 and 1973). The average period for stabilization of the disease was 13,2 months with radium and 20,4 months with irridium. Unfortunately, this does not give a permanent cure and, for irridium 192, the percentage of successes is only 30 p. 100 after 5 years. Logically, radiotherapy with irridium 192 should only be used when other methods have failed. The progressive accumulation of radio-lesions may, in fact, make the use of other techniques impossible.", "PMID": 970864}
{"id": "pubmed23n0032_8224", "title": "[Enumeration of the cestodes of plankton and marine invertebrates. 9th contribution].", "content": "This 9th Contribution concerns Cestodes obtained from: A Lamellibranchiata, B Gastropoda, C Pteropoda, D Cephalopoda, E Crustacea.", "contents": "[Enumeration of the cestodes of plankton and marine invertebrates. 9th contribution]. This 9th Contribution concerns Cestodes obtained from: A Lamellibranchiata, B Gastropoda, C Pteropoda, D Cephalopoda, E Crustacea.", "PMID": 970867}
{"id": "pubmed23n0032_8225", "title": "[Neopolystoma euzeti n. sp. (Monogenea, Polystomatidae) first member of the genus Neopolystoma Price, 1939 in Africa (author's transl)].", "content": "Neopolystoma euzeti n. sp., a parasite of the urinary bladder and the rectum of the freshwater tortoise Clemys caspica var. leprosa in Tunisia, is mainly characterized by its genital spines in high number (33 to 36) and the very important extension of the vitellaria. It is the first species of the genus Neopolystoma Price, 1939, to be described in Africa, the others being known from North America, Japan and Eastern Siberia.", "contents": "[Neopolystoma euzeti n. sp. (Monogenea, Polystomatidae) first member of the genus Neopolystoma Price, 1939 in Africa (author's transl)]. Neopolystoma euzeti n. sp., a parasite of the urinary bladder and the rectum of the freshwater tortoise Clemys caspica var. leprosa in Tunisia, is mainly characterized by its genital spines in high number (33 to 36) and the very important extension of the vitellaria. It is the first species of the genus Neopolystoma Price, 1939, to be described in Africa, the others being known from North America, Japan and Eastern Siberia.", "PMID": 970868}
{"id": "pubmed23n0032_8226", "title": "[Hymenolepis guadeloupensis n. sp., new cestode of domestic duck Anas boschas, Linn\u00e9 (author's transl)].", "content": "Description of a new species of Hymenolepididae, Hymenolepis guadeloupensis n. sp., parasite of domestic duck, Anas boschas Linn\u00e9, in Guadalupa. This species, actually, cannot be completely identified to Hymenolepis fista Meggitt, 1933, Cestode of asiatic Cotton ygmy goose, because the latter is insufficiently described.", "contents": "[Hymenolepis guadeloupensis n. sp., new cestode of domestic duck Anas boschas, Linn\u00e9 (author's transl)]. Description of a new species of Hymenolepididae, Hymenolepis guadeloupensis n. sp., parasite of domestic duck, Anas boschas Linn\u00e9, in Guadalupa. This species, actually, cannot be completely identified to Hymenolepis fista Meggitt, 1933, Cestode of asiatic Cotton ygmy goose, because the latter is insufficiently described.", "PMID": 970866}
{"id": "pubmed23n0032_8227", "title": "[Experimental schistosomiasis. II. Infection of Biophalaria glabrata by 2 miracidia of Schistosoma mansoni].", "content": "The authors have infected 127 B. glabrata by 2 miracidia of S. mansoni, either on the Same day, or introducing a second miracidium after 3, 7 and 16 days. 1 All the groups of planorbid snails had a low percentage of positivity (31 to 41 %). 2 The first cercarial emissions, in the 4 groups were scattered in the time, the delay being in correlation with the second infection. 3 In the snails reinfected after 3 and 7 days occured the highest emissions, those with the simultaneous double infection or second infection delayed to 16 days, had the lesser emissions. 4 An interval cycle of about 3 weeks was discovered for the highest emissions. All those phenomena are probably due to competition between the sporocysts born from both miracidia. Moreover, evident reduction of the fecundity in the positive snails were shown only after the beginning of the cercarial emissions, while a normal, or even increased fecundity was established in the prepatent period of the infected snails.", "contents": "[Experimental schistosomiasis. II. Infection of Biophalaria glabrata by 2 miracidia of Schistosoma mansoni]. The authors have infected 127 B. glabrata by 2 miracidia of S. mansoni, either on the Same day, or introducing a second miracidium after 3, 7 and 16 days. 1 All the groups of planorbid snails had a low percentage of positivity (31 to 41 %). 2 The first cercarial emissions, in the 4 groups were scattered in the time, the delay being in correlation with the second infection. 3 In the snails reinfected after 3 and 7 days occured the highest emissions, those with the simultaneous double infection or second infection delayed to 16 days, had the lesser emissions. 4 An interval cycle of about 3 weeks was discovered for the highest emissions. All those phenomena are probably due to competition between the sporocysts born from both miracidia. Moreover, evident reduction of the fecundity in the positive snails were shown only after the beginning of the cercarial emissions, while a normal, or even increased fecundity was established in the prepatent period of the infected snails.", "PMID": 970869}
{"id": "pubmed23n0032_8228", "title": "The different components of gastric emptying after gastric surgery.", "content": "Gastric emptying of liquid and solid meals was studied before and after operation in 22 male patients who were admitted to a prospective randomized trial of truncal vagotomy and antrectomy and proximal gastric vagotomy in the treatment of duodenal ulceration. After operation the emptying of both solid and liquid meals was biphasic, with a passive early phase and a later controlled active phase. A significant increase in early liquid emptying was produced by both operations, and after truncal vagotomy and antrectomy also with the solid meal. Active emptying of the liquid meals was unaffected by either operation, but both operations significantly prolonged the active emptying of the solid meals. The clinical symptoms of dumping were related to rapid early liquid emptying (cascading) and those of gastric retention were related to delayed active emptying of the solid meal. These findings explain how the same operation can produce the opposing symptom complexes of dumping and gastric retention and how both sets of symptoms can occur in the same individual.", "contents": "The different components of gastric emptying after gastric surgery. Gastric emptying of liquid and solid meals was studied before and after operation in 22 male patients who were admitted to a prospective randomized trial of truncal vagotomy and antrectomy and proximal gastric vagotomy in the treatment of duodenal ulceration. After operation the emptying of both solid and liquid meals was biphasic, with a passive early phase and a later controlled active phase. A significant increase in early liquid emptying was produced by both operations, and after truncal vagotomy and antrectomy also with the solid meal. Active emptying of the liquid meals was unaffected by either operation, but both operations significantly prolonged the active emptying of the solid meals. The clinical symptoms of dumping were related to rapid early liquid emptying (cascading) and those of gastric retention were related to delayed active emptying of the solid meal. These findings explain how the same operation can produce the opposing symptom complexes of dumping and gastric retention and how both sets of symptoms can occur in the same individual.", "PMID": 970882}
{"id": "pubmed23n0032_8229", "title": "The diagnosis and assessment of abdominal aortic aneurysms by ultrasonography.", "content": "A study of 150 patients examined by ultrasonography is described. It was designed specifically to diagnose and investigate abdominal aortic aneurysms. Sixty-four aneurysms were found. Ultrasonography was useful in distinguishing aneurysms from other conditions and there was a high degree of correlation between size measured by scanning and operative size. In addition to the demonstration of rupture it was possible to perform serial measurements. The technique is simple and quick and causes minimal disturbance to the patient.", "contents": "The diagnosis and assessment of abdominal aortic aneurysms by ultrasonography. A study of 150 patients examined by ultrasonography is described. It was designed specifically to diagnose and investigate abdominal aortic aneurysms. Sixty-four aneurysms were found. Ultrasonography was useful in distinguishing aneurysms from other conditions and there was a high degree of correlation between size measured by scanning and operative size. In addition to the demonstration of rupture it was possible to perform serial measurements. The technique is simple and quick and causes minimal disturbance to the patient.", "PMID": 970885}
{"id": "pubmed23n0032_8230", "title": "Hazards in the management of large intra-abdominal tumours.", "content": "Whatever their nature, large intra-abdominal tumours interfere with respiratory and circulatory function by producing elevation and splinting of the diaphragm and partial occlusion of the inferior vena cava. The main hazards involved in removing such tumours are consequences of abdominal decompression, which may produce a labile cardiovascular state, respiratory difficulties, and rapid intestinal distension. A knowledge of the deranged physiology and its management may avert these complications. Careful preparation, modification of anaesthetic technique, postoperative ventilation, and external abdominal compression are important. To illustrate the discussion two cases of large ovarian cysts are described.", "contents": "Hazards in the management of large intra-abdominal tumours. Whatever their nature, large intra-abdominal tumours interfere with respiratory and circulatory function by producing elevation and splinting of the diaphragm and partial occlusion of the inferior vena cava. The main hazards involved in removing such tumours are consequences of abdominal decompression, which may produce a labile cardiovascular state, respiratory difficulties, and rapid intestinal distension. A knowledge of the deranged physiology and its management may avert these complications. Careful preparation, modification of anaesthetic technique, postoperative ventilation, and external abdominal compression are important. To illustrate the discussion two cases of large ovarian cysts are described.", "PMID": 970886}
{"id": "pubmed23n0032_8231", "title": "Subtotal amputation for carcinoma of the penis with reconstruction of penile stump.", "content": "Partial and total amputation are well-accepted procedures for the surgical treatment of carcinoma of the penis. In certain cases after adequate amputation the residual penile stump is too small from the penoscrotal junction to offer useful function, and a total amputation is therefore usually performed. In such cases a subtotal amputation with reconstruction of the penile stump avoids a perineal urethrostomy and permits micturition in the erect posture.", "contents": "Subtotal amputation for carcinoma of the penis with reconstruction of penile stump. Partial and total amputation are well-accepted procedures for the surgical treatment of carcinoma of the penis. In certain cases after adequate amputation the residual penile stump is too small from the penoscrotal junction to offer useful function, and a total amputation is therefore usually performed. In such cases a subtotal amputation with reconstruction of the penile stump avoids a perineal urethrostomy and permits micturition in the erect posture.", "PMID": 970887}
{"id": "pubmed23n0032_8232", "title": "Bioplast fibrin buttons for liver biopsy and partial hepatic resection.", "content": "Absorbable buttons made from fibrin (Bioplast buttons) have been used to facilitate liver biopsy or to control haemorrhage from the liver in 3 dogs and 14 patients. The buttons are easy to use, effective, and readily absorbed.", "contents": "Bioplast fibrin buttons for liver biopsy and partial hepatic resection. Absorbable buttons made from fibrin (Bioplast buttons) have been used to facilitate liver biopsy or to control haemorrhage from the liver in 3 dogs and 14 patients. The buttons are easy to use, effective, and readily absorbed.", "PMID": 970888}
{"id": "pubmed23n0032_8233", "title": "Effect of 2 br-alpha-ergocryptin (CB 154) on serum prolactin and the clinical picture in a case of progressive gigantomastia in pregnancy.", "content": "A 24 year old woman who had epilepsy since the age of 7 years and who was still using antiepileptics, developed an excessive mamary growth (gigantomastia) during pregnancy. Treatment with CB 154 (Sandoz), 2 Br-alpha-ergocryptin in a dose of 2.5 mg three times a day from the 27th week of pregnancy, resulted in prompt improvement with a corresponding decrease in the plasma prolactin, return of the breast temperature to normal, and disappearance of the EEG-abnormalities. After parturition and subsequent mammoplasty the woman soon became pregant again. During this pregnancy, which terminated in the birth of a normal child, treatment with CB 154 was started early and continued throughout pregnancy with success and without side effects.", "contents": "Effect of 2 br-alpha-ergocryptin (CB 154) on serum prolactin and the clinical picture in a case of progressive gigantomastia in pregnancy. A 24 year old woman who had epilepsy since the age of 7 years and who was still using antiepileptics, developed an excessive mamary growth (gigantomastia) during pregnancy. Treatment with CB 154 (Sandoz), 2 Br-alpha-ergocryptin in a dose of 2.5 mg three times a day from the 27th week of pregnancy, resulted in prompt improvement with a corresponding decrease in the plasma prolactin, return of the breast temperature to normal, and disappearance of the EEG-abnormalities. After parturition and subsequent mammoplasty the woman soon became pregant again. During this pregnancy, which terminated in the birth of a normal child, treatment with CB 154 was started early and continued throughout pregnancy with success and without side effects.", "PMID": 970897}
{"id": "pubmed23n0032_8234", "title": "Multiple mesenteric cysts diagnosed by ultrasound. A case report.", "content": "The diagnostic value of ultrasound in mesenteric cysts in infancy and childhood is demonstrated by a case report of a 4 year old girl with multiple cysts in the transverse mesocolon and with no other symptoms or signs than an enlargement of the abdomen. Neither clinical nor radiological examination could give the diagnosis, whereas ultrasound examination promptly revealed large, thin-walled, partly locular, cystic masses.", "contents": "Multiple mesenteric cysts diagnosed by ultrasound. A case report. The diagnostic value of ultrasound in mesenteric cysts in infancy and childhood is demonstrated by a case report of a 4 year old girl with multiple cysts in the transverse mesocolon and with no other symptoms or signs than an enlargement of the abdomen. Neither clinical nor radiological examination could give the diagnosis, whereas ultrasound examination promptly revealed large, thin-walled, partly locular, cystic masses.", "PMID": 970898}
{"id": "pubmed23n0032_8235", "title": "A study of cumulative effects and recovery from anaesthesia with intermittent doses of althesin. A comparison with methohexitone.", "content": "The cumulative effects and post-anaesthetic recovery of Althesin were studied by comparing the drug with methohexitone in a series of 60 patients undergoing surgery for varicose veins. Anaesthesia was maintained with each anaesthetic agent in 30 patients by administration of intermittent doses of the respective drugs in accordance with the surgical stimulus. When repeat doses were required at intervals of 2 to 5 minutes, the fall off in requirements was observed with both anaesthetics, more distinctly with Althesin than with methohexitone. No significant difference could be observed in the time required for immediate awakening after the two drugs. However, the majority of the patients anaesthetized with Althesin displayed a peculiar lack of mental clarity for a short period after recovering consciousness. The recovery from anaesthesia was studied by various tests and observations. Recovery after Althesin appeared to proceed slightly faster than after methohexitone. When Althesin was required in high total dosage (exceeding 150 mu1/kg), the immediate awakening was associated with emotional upset and confusion. Frequency of nausea and vomiting after anaesthesia was considerably higher in the Althesin group than in the methohexitone group. These symptoms might be toxic due to the excessive dosage given. Using the induction time as a basis for calculation of the potency ratio, Althesin and methohexiton were found to have the ratio of 1:33 (expressed in mu1/kg : mg/kg).", "contents": "A study of cumulative effects and recovery from anaesthesia with intermittent doses of althesin. A comparison with methohexitone. The cumulative effects and post-anaesthetic recovery of Althesin were studied by comparing the drug with methohexitone in a series of 60 patients undergoing surgery for varicose veins. Anaesthesia was maintained with each anaesthetic agent in 30 patients by administration of intermittent doses of the respective drugs in accordance with the surgical stimulus. When repeat doses were required at intervals of 2 to 5 minutes, the fall off in requirements was observed with both anaesthetics, more distinctly with Althesin than with methohexitone. No significant difference could be observed in the time required for immediate awakening after the two drugs. However, the majority of the patients anaesthetized with Althesin displayed a peculiar lack of mental clarity for a short period after recovering consciousness. The recovery from anaesthesia was studied by various tests and observations. Recovery after Althesin appeared to proceed slightly faster than after methohexitone. When Althesin was required in high total dosage (exceeding 150 mu1/kg), the immediate awakening was associated with emotional upset and confusion. Frequency of nausea and vomiting after anaesthesia was considerably higher in the Althesin group than in the methohexitone group. These symptoms might be toxic due to the excessive dosage given. Using the induction time as a basis for calculation of the potency ratio, Althesin and methohexiton were found to have the ratio of 1:33 (expressed in mu1/kg : mg/kg).", "PMID": 970899}
{"id": "pubmed23n0032_8236", "title": "Use of 131I-toluidine blue in radionuclide imaging of enlarged parathyroid glands.", "content": "Parathyroid scintigraphy with 131I-labelled toluidine blue was performed in 40 patients, 30 of whom were operated for suspected hyperparathyroidism. Hyperplastic glands or adenomas could be localized by scintigraphy in 18 of 27 patients who proved to have hyperparathyroidism. The correlation between scintigraphy and operative finding was always correct when the parathyroids weighed more than 2 g. Scintigraphy was correctly negative in one, falsely negative in 6, and falsely positive in 5 of the operated patients. In a group of 10 unoperated patients, scintigraphy was positive in one of the 3 patients with suspected hyperparathyroidism, but negative in the others. Although 131I-toluidine blue is not ideal as a scintigraphic agent for the parathyroids, we are of the opinion that it represents an improvement compared with 75Se-selenomethionine.", "contents": "Use of 131I-toluidine blue in radionuclide imaging of enlarged parathyroid glands. Parathyroid scintigraphy with 131I-labelled toluidine blue was performed in 40 patients, 30 of whom were operated for suspected hyperparathyroidism. Hyperplastic glands or adenomas could be localized by scintigraphy in 18 of 27 patients who proved to have hyperparathyroidism. The correlation between scintigraphy and operative finding was always correct when the parathyroids weighed more than 2 g. Scintigraphy was correctly negative in one, falsely negative in 6, and falsely positive in 5 of the operated patients. In a group of 10 unoperated patients, scintigraphy was positive in one of the 3 patients with suspected hyperparathyroidism, but negative in the others. Although 131I-toluidine blue is not ideal as a scintigraphic agent for the parathyroids, we are of the opinion that it represents an improvement compared with 75Se-selenomethionine.", "PMID": 970900}
{"id": "pubmed23n0032_8237", "title": "Spigelian hernia.", "content": "Six consecutive patients with Spigelian hernia treated operatively over a period of ten years are reported. There were no complications, no deaths, and no recurrence in this series. Recognition of this rather rare lesion may be difficult, but it is important because of its quite high predisposition to incarceration and strangulation. The main pathological and clinical features are discussed, and the literature briefly reviewed.", "contents": "Spigelian hernia. Six consecutive patients with Spigelian hernia treated operatively over a period of ten years are reported. There were no complications, no deaths, and no recurrence in this series. Recognition of this rather rare lesion may be difficult, but it is important because of its quite high predisposition to incarceration and strangulation. The main pathological and clinical features are discussed, and the literature briefly reviewed.", "PMID": 970901}
{"id": "pubmed23n0032_8238", "title": "The effect of prophylactic use of furosemide on renal function during open heart surgery.", "content": "Forty-five patients who underwent open heart surgery were divided into a short-perfusion group (SPG, 21 patients) with a perfusion time shorter than 60 minutes and a long-perfusion group (LPG, 24 patients) with a perfusion time longer than 60 minutes. Nine patients in the SPG and 13 in the LPG received furosemide prophylactically prior to the perfusion. The furosemide dose was 20-60 mg. During the perfusion and postoperatively all patients were given furosemide when necessary, i.e. when the volume for diuresis per hour declined below 40 ml/h. The patients who received prophylactic furosemide in the LPG subsequently had clearly less need for furosemide (3.9 mg/h) than the control subjects (7.9 mg/h/3 day). The patients with furosemide prophylaxis in the LPG had significantly ( less than 0.05) higher creatinine clearance and lower serum creatinine values during the postoperative night period and on the 3rd day. In perfusions lasting less than 60 minutes the patients with furosemide prophylaxis had significantly higher urine flow (p less than 0.001), sodium excretion (p less than 0.001) and potassium excretio-n (p less than 0.01) during bypass surgery and postoperatively compared with that of controls. According to our findings, the prophylactic use of furosemide had a beneficial effect on glomerular filtration rate (endogenous creatinine clearance) and postoperative serum creatinine level in LPG. In perfusion shorter than 60 minutes furosemide prophylaxis may be harmful owing to the increased excretion of water, sodium and potassium.", "contents": "The effect of prophylactic use of furosemide on renal function during open heart surgery. Forty-five patients who underwent open heart surgery were divided into a short-perfusion group (SPG, 21 patients) with a perfusion time shorter than 60 minutes and a long-perfusion group (LPG, 24 patients) with a perfusion time longer than 60 minutes. Nine patients in the SPG and 13 in the LPG received furosemide prophylactically prior to the perfusion. The furosemide dose was 20-60 mg. During the perfusion and postoperatively all patients were given furosemide when necessary, i.e. when the volume for diuresis per hour declined below 40 ml/h. The patients who received prophylactic furosemide in the LPG subsequently had clearly less need for furosemide (3.9 mg/h) than the control subjects (7.9 mg/h/3 day). The patients with furosemide prophylaxis in the LPG had significantly ( less than 0.05) higher creatinine clearance and lower serum creatinine values during the postoperative night period and on the 3rd day. In perfusions lasting less than 60 minutes the patients with furosemide prophylaxis had significantly higher urine flow (p less than 0.001), sodium excretion (p less than 0.001) and potassium excretio-n (p less than 0.01) during bypass surgery and postoperatively compared with that of controls. According to our findings, the prophylactic use of furosemide had a beneficial effect on glomerular filtration rate (endogenous creatinine clearance) and postoperative serum creatinine level in LPG. In perfusion shorter than 60 minutes furosemide prophylaxis may be harmful owing to the increased excretion of water, sodium and potassium.", "PMID": 970902}
{"id": "pubmed23n0032_8239", "title": "Surgical considerations in tracheal resection for tracheal stenosis.", "content": "Five patients were succesfully operated upon for tracheal stricture following prolonged assisted ventilation with a cuffed tracheal tube. The operative exposures and techniques to be recommended with relation to site and extent of tracheal stenosis are discussed. Most tracheal resections can be carried out through a low cervical incision. Lesions of the lower part of the trachea (5 cm or less from the carina) are best approached through a right posterolateral thoracotomy incision. A stenosed tracheal segment of 4 cm or less can be resected easily by both routes without undue tension on the anastomotic line. The use of interrupted Prolene sutures is proposed for the tracheal anastomosis, because it produces much less tissue reaction than chromic catgut.", "contents": "Surgical considerations in tracheal resection for tracheal stenosis. Five patients were succesfully operated upon for tracheal stricture following prolonged assisted ventilation with a cuffed tracheal tube. The operative exposures and techniques to be recommended with relation to site and extent of tracheal stenosis are discussed. Most tracheal resections can be carried out through a low cervical incision. Lesions of the lower part of the trachea (5 cm or less from the carina) are best approached through a right posterolateral thoracotomy incision. A stenosed tracheal segment of 4 cm or less can be resected easily by both routes without undue tension on the anastomotic line. The use of interrupted Prolene sutures is proposed for the tracheal anastomosis, because it produces much less tissue reaction than chromic catgut.", "PMID": 970903}
{"id": "pubmed23n0032_8240", "title": "The effect of furosemide on renal blood flow and renal tissue oxygen tension in dogs.", "content": "The effect of furosemide (2 mg/kg i.v.) on blood flowin the renal artery and the tissue oxygen tension of the renal cortex and medulla was investigated in six dogs. The flow was measured with an electromagnetic flowmeter and the tissue oxygen tension with IBC tissue oxygen electrodes. The flow in the renal artery increased significantly (p less than 0.05) 5-15 minutes after furosemide administration. 40 minutes after the injection the flow had returned to its initial level. The tissue oxygen tension of both the cortex and the medulla showed significant elevation following furosemide administration. The maximum increase of tissue oxygen tension was recorded 10-20 minutes after the injection. The oxygen tension values exceeded the initial level by 22% in the cortex and by 48% (p less than 0.001) in the medulla. Simultaneously, with the changes in oxygen tension, urine output increase considerably and urine osmolality declined. Application of these renal effects of furosemide in clinical work, particularly in cardiopulmonary bypass surgery, is discussed.", "contents": "The effect of furosemide on renal blood flow and renal tissue oxygen tension in dogs. The effect of furosemide (2 mg/kg i.v.) on blood flowin the renal artery and the tissue oxygen tension of the renal cortex and medulla was investigated in six dogs. The flow was measured with an electromagnetic flowmeter and the tissue oxygen tension with IBC tissue oxygen electrodes. The flow in the renal artery increased significantly (p less than 0.05) 5-15 minutes after furosemide administration. 40 minutes after the injection the flow had returned to its initial level. The tissue oxygen tension of both the cortex and the medulla showed significant elevation following furosemide administration. The maximum increase of tissue oxygen tension was recorded 10-20 minutes after the injection. The oxygen tension values exceeded the initial level by 22% in the cortex and by 48% (p less than 0.001) in the medulla. Simultaneously, with the changes in oxygen tension, urine output increase considerably and urine osmolality declined. Application of these renal effects of furosemide in clinical work, particularly in cardiopulmonary bypass surgery, is discussed.", "PMID": 970904}
{"id": "pubmed23n0032_8241", "title": "The effect of furosemide on potassium balance in open heart surgery.", "content": "Potassium balance was investigated in forty-five patients who underwent open-heart surgery. The patients were divided into a short-perfusion group (SPG, 24 patients) with a perfusion time shorter than 60 minutes and a long-perfusion group (LPG, 24 patients) with a perfusion time longer than 60 minutes. Nine patients in the SPG and 13 in the LPG received furosemide prophylactically prior to the perfusion. The furosemide dose was 20-60 mg. During the perfusion andpostoperatively all patients were given furosemide when necessary, i.e. when the urine output declined below 0.5 ml/kg/h. In both the short- and the long-perfusion groups potassium balance was negative on the day of operation and the following day. On the day of operation the patients in the SPG with furosemide prophylaxis had a negative potassium balance of 116 mmol and without prophylaxis of 33 mmol. The corresponding values in the LPG were 35 and 31 mmol. On the first postoperative day the values were 33 mmol and 50 mmol in the SPG and 29 mmol and 36 mmol in the LPG, respectively. The serum potassium level declined slightly in all the groups; the greatest decline was recorded for the SPG patients with prophylactic furosemide administration.", "contents": "The effect of furosemide on potassium balance in open heart surgery. Potassium balance was investigated in forty-five patients who underwent open-heart surgery. The patients were divided into a short-perfusion group (SPG, 24 patients) with a perfusion time shorter than 60 minutes and a long-perfusion group (LPG, 24 patients) with a perfusion time longer than 60 minutes. Nine patients in the SPG and 13 in the LPG received furosemide prophylactically prior to the perfusion. The furosemide dose was 20-60 mg. During the perfusion andpostoperatively all patients were given furosemide when necessary, i.e. when the urine output declined below 0.5 ml/kg/h. In both the short- and the long-perfusion groups potassium balance was negative on the day of operation and the following day. On the day of operation the patients in the SPG with furosemide prophylaxis had a negative potassium balance of 116 mmol and without prophylaxis of 33 mmol. The corresponding values in the LPG were 35 and 31 mmol. On the first postoperative day the values were 33 mmol and 50 mmol in the SPG and 29 mmol and 36 mmol in the LPG, respectively. The serum potassium level declined slightly in all the groups; the greatest decline was recorded for the SPG patients with prophylactic furosemide administration.", "PMID": 970905}
{"id": "pubmed23n0032_8242", "title": "Blood alcohol levels in injury victims at the emergency station of a rural central hospital.", "content": "Blood alcohol was determined in 182 consecutive adult injury victims at the emergency station of a rural central hospital during a fortnight in March 1974. In 30% of them alcohol was found in the blood. The percentage was 38 in males and 15 in females. No significant differences in alcohol involvement (AI) between social classes were noted. During the night 62% of the patients had ingested alcohol. The injuries on Saturdays were more often alcohol-involved (54%) than on other days of the week (26%). AI was very frequent (86%) in the victims of fights, assaults, and suicide attempts, while it was only rarely (9%) seen in industrial accidents. AI was clinically detected in 72% of the cases with positive blood alcohol concentrations. It can be concluded from the results of this and other studies referred to, that the AI rate for fresh injuries of adults at the Pinnish emergency stations was about 30-35%.", "contents": "Blood alcohol levels in injury victims at the emergency station of a rural central hospital. Blood alcohol was determined in 182 consecutive adult injury victims at the emergency station of a rural central hospital during a fortnight in March 1974. In 30% of them alcohol was found in the blood. The percentage was 38 in males and 15 in females. No significant differences in alcohol involvement (AI) between social classes were noted. During the night 62% of the patients had ingested alcohol. The injuries on Saturdays were more often alcohol-involved (54%) than on other days of the week (26%). AI was very frequent (86%) in the victims of fights, assaults, and suicide attempts, while it was only rarely (9%) seen in industrial accidents. AI was clinically detected in 72% of the cases with positive blood alcohol concentrations. It can be concluded from the results of this and other studies referred to, that the AI rate for fresh injuries of adults at the Pinnish emergency stations was about 30-35%.", "PMID": 970906}
{"id": "pubmed23n0032_8243", "title": "Blood alcohol levels in a series of injured patients with special reference to accident and type of injury.", "content": "Blood alcohol was determined in 1012 injury victims. It was found that the alcohol involvement (AI) rate was 37% in the total number of patients, 19% in industrial, 38% in traffic, 36% in home, 45% in other freetime accidents, and 69% in the victims of fights, assaults, and suicide attempts. The most common external cause of injury was falling (447 cases) with an AI rate of 38%, while the 172 other nontraffic true accident cases had a rate of 24%. The road and stairs were the usual places of falls while intoxicated. Head injuries had a high (47%) and upper extremity injuries a low (25%) AI rate. Tibial and ankle fractures were also very often associated with alcohol intake. Contrarily to traffic and industrial accidents the slightly injured at home and in other freetime environments had been drinking more frequently than the severely injured. The data suggest that alcohol is a powerful contributing factor in leisure-time injuries. Head and low leg are especially vulnerable in drunken persons. Not only driving but even when walking while intoxicated means taking risks.", "contents": "Blood alcohol levels in a series of injured patients with special reference to accident and type of injury. Blood alcohol was determined in 1012 injury victims. It was found that the alcohol involvement (AI) rate was 37% in the total number of patients, 19% in industrial, 38% in traffic, 36% in home, 45% in other freetime accidents, and 69% in the victims of fights, assaults, and suicide attempts. The most common external cause of injury was falling (447 cases) with an AI rate of 38%, while the 172 other nontraffic true accident cases had a rate of 24%. The road and stairs were the usual places of falls while intoxicated. Head injuries had a high (47%) and upper extremity injuries a low (25%) AI rate. Tibial and ankle fractures were also very often associated with alcohol intake. Contrarily to traffic and industrial accidents the slightly injured at home and in other freetime environments had been drinking more frequently than the severely injured. The data suggest that alcohol is a powerful contributing factor in leisure-time injuries. Head and low leg are especially vulnerable in drunken persons. Not only driving but even when walking while intoxicated means taking risks.", "PMID": 970907}
{"id": "pubmed23n0032_8244", "title": "Use of hemodialysis and forced diuresis in the treatment of poisoning.", "content": "This paper is a brief commentary of the role of forced diuresis and hemodialysis in the treatment of acute poisoning. The importance of informed conservative management is stressed. Rationale and methods of forced diuresis and urinary alkalinization are presented. Dialysis is reserved for special situations where shortening the poison-patient contact time is indicated.", "contents": "Use of hemodialysis and forced diuresis in the treatment of poisoning. This paper is a brief commentary of the role of forced diuresis and hemodialysis in the treatment of acute poisoning. The importance of informed conservative management is stressed. Rationale and methods of forced diuresis and urinary alkalinization are presented. Dialysis is reserved for special situations where shortening the poison-patient contact time is indicated.", "PMID": 970924}
{"id": "pubmed23n0032_8245", "title": "Serum cholesterol esterifying activity in kwasiorkor.", "content": "Serum lipid levels were determined in 30 children with kwashiorkor and in 30 healthy children of comparable age. The serum concentrations of unesterified and esterified cholesterol, albumin and the cholesterol esterifying activity (CEA) were also measured in children with kwashiorkor before treatment and after recovery. All serum lipid fractions were significantly lower in kwashiorkor than in the normal children. After treatment and recovery, serum lipid levels were comparable to those observed in normal children. There was also a significant increase in serum cholesterol esterifying activity (CEA) following recovery from kwashiorkor.", "contents": "Serum cholesterol esterifying activity in kwasiorkor. Serum lipid levels were determined in 30 children with kwashiorkor and in 30 healthy children of comparable age. The serum concentrations of unesterified and esterified cholesterol, albumin and the cholesterol esterifying activity (CEA) were also measured in children with kwashiorkor before treatment and after recovery. All serum lipid fractions were significantly lower in kwashiorkor than in the normal children. After treatment and recovery, serum lipid levels were comparable to those observed in normal children. There was also a significant increase in serum cholesterol esterifying activity (CEA) following recovery from kwashiorkor.", "PMID": 970925}
{"id": "pubmed23n0032_8246", "title": "Fluorocarbon toxicity: aerosol deaths and anaesthetic reactions.", "content": "Twelve cases of death due to fluorocarbon inhalation have been seen in a four year period 1971-1975. The source of the material has been commercial spray cans. The postmortem examinations are nonspecific, but a history of excitement or sudden death is often elicited. Confirmation of the cause of death was made by demonstration of a halogenated hydrocarbon in the blood by gas chromatography.", "contents": "Fluorocarbon toxicity: aerosol deaths and anaesthetic reactions. Twelve cases of death due to fluorocarbon inhalation have been seen in a four year period 1971-1975. The source of the material has been commercial spray cans. The postmortem examinations are nonspecific, but a history of excitement or sudden death is often elicited. Confirmation of the cause of death was made by demonstration of a halogenated hydrocarbon in the blood by gas chromatography.", "PMID": 970926}
{"id": "pubmed23n0032_8247", "title": "Mechanism of D-alanine production by Corynebacterium fascians.", "content": "The amounts of extracellular D-alanine accumulated by Corynebacterium fascians ATCC 21950 in a medium containing glycerol as the sole carbon source is increased to almost 12 mg/ml by adding pyruvate to the medium. Cell-free extracts of C. fascians were shown to possess both L-alanine dehydrogenase and alanine racemase activities. These results indicated that a mechanism exists that allows this microorganism to synthesize D-alanine from pyruvate. A study comparing the optical purity of the intracellular alanine and the extracellular alanine suggested that the cell membrane possesses the stereospecific permeability for D-alanine. Thus, it may be concluded that L-alanine is first formed from pyruvate by L-alanine dehydrogenase and then converted to D-alanine by racemase inside the cells. Subsequently, only D-alanine leaks out stereospecifically through the cell membrane, and large amounts of D-alanine accumulate in the extracellular medium.", "contents": "Mechanism of D-alanine production by Corynebacterium fascians. The amounts of extracellular D-alanine accumulated by Corynebacterium fascians ATCC 21950 in a medium containing glycerol as the sole carbon source is increased to almost 12 mg/ml by adding pyruvate to the medium. Cell-free extracts of C. fascians were shown to possess both L-alanine dehydrogenase and alanine racemase activities. These results indicated that a mechanism exists that allows this microorganism to synthesize D-alanine from pyruvate. A study comparing the optical purity of the intracellular alanine and the extracellular alanine suggested that the cell membrane possesses the stereospecific permeability for D-alanine. Thus, it may be concluded that L-alanine is first formed from pyruvate by L-alanine dehydrogenase and then converted to D-alanine by racemase inside the cells. Subsequently, only D-alanine leaks out stereospecifically through the cell membrane, and large amounts of D-alanine accumulate in the extracellular medium.", "PMID": 970932}
{"id": "pubmed23n0032_8248", "title": "Acetylene reduction (nitrogen fixation) associated with corn inoculated with Spirillum.", "content": "Sorghum and corn breeding lines were grown in soil in field and greenhouse experiments with and without an inoculum of N2-fixing in Spirillum strains from Brazil. Estimated rates of N2 fixation associated with field-grown corn and sorghum plants were less than 4 g of N2/ha per day. The mean estimated N2-fixation rates determined on segments of roots from corn inoculated with Spirillum and grown in the greenhouse at 24 to 27 degrees C were 15 g of N2/ha per day (16 inbreds), 25 g of N2/ha per day (six hybrids), and 165 g of N2/ha per day for one hybird which was heavily inoculated. The corresponding mean rates determined from measurements of in situ cultures of the same series of corn plants (i.e., 16 inbreds, six hybrids, and one heavily inoculated hybrid) were 0.4, 2.3, and 1.1 g of N2/ha per day, respectively. Lower rates of C2H2 reduction were associated with control corn cultures which had been treated with autoclaved Spirillum than with cultures inoculated with live Spirillum. No C2H2 reduction was detected in plant cultures treated with ammonium nitrate. Numbers of nitrogen-fixing bacteria on excised roots of corn plants increased an average of about 30-fold during an overnight preincubation period, and as a result acetylene reduction assays of root samples after preincubation failed to serve as a valid basis for estimating N2 fixation by corn in pot cultures. Plants grown without added nitrogen either with or without inoculum exhibited severe symptoms of nitrogen deficiency and in most cases produced significantly less dry weight than those supplied with fixed nitrogen. Although substantial rates of C2H2 reduction by excised corn roots were observed after preincubation under limited oxygen, the yield and nitrogen content of inoculated plants and the C2H2-reduction rates by inoculated pot cultures of corn, in situ, provided no evidence of appreciable N2 fixation.", "contents": "Acetylene reduction (nitrogen fixation) associated with corn inoculated with Spirillum. Sorghum and corn breeding lines were grown in soil in field and greenhouse experiments with and without an inoculum of N2-fixing in Spirillum strains from Brazil. Estimated rates of N2 fixation associated with field-grown corn and sorghum plants were less than 4 g of N2/ha per day. The mean estimated N2-fixation rates determined on segments of roots from corn inoculated with Spirillum and grown in the greenhouse at 24 to 27 degrees C were 15 g of N2/ha per day (16 inbreds), 25 g of N2/ha per day (six hybrids), and 165 g of N2/ha per day for one hybird which was heavily inoculated. The corresponding mean rates determined from measurements of in situ cultures of the same series of corn plants (i.e., 16 inbreds, six hybrids, and one heavily inoculated hybrid) were 0.4, 2.3, and 1.1 g of N2/ha per day, respectively. Lower rates of C2H2 reduction were associated with control corn cultures which had been treated with autoclaved Spirillum than with cultures inoculated with live Spirillum. No C2H2 reduction was detected in plant cultures treated with ammonium nitrate. Numbers of nitrogen-fixing bacteria on excised roots of corn plants increased an average of about 30-fold during an overnight preincubation period, and as a result acetylene reduction assays of root samples after preincubation failed to serve as a valid basis for estimating N2 fixation by corn in pot cultures. Plants grown without added nitrogen either with or without inoculum exhibited severe symptoms of nitrogen deficiency and in most cases produced significantly less dry weight than those supplied with fixed nitrogen. Although substantial rates of C2H2 reduction by excised corn roots were observed after preincubation under limited oxygen, the yield and nitrogen content of inoculated plants and the C2H2-reduction rates by inoculated pot cultures of corn, in situ, provided no evidence of appreciable N2 fixation.", "PMID": 970933}
{"id": "pubmed23n0032_8249", "title": "Disorders of granulocytes induced by toxic agents.", "content": "The effect of various toxic agents on granulocytes or their precursors is reviewed with emphasis on hypoplastic anemia, granylocytopenia (toxic, idiosyncratic and immune), leukemia and morphologic cytoplasmic and nuclear changes.", "contents": "Disorders of granulocytes induced by toxic agents. The effect of various toxic agents on granulocytes or their precursors is reviewed with emphasis on hypoplastic anemia, granylocytopenia (toxic, idiosyncratic and immune), leukemia and morphologic cytoplasmic and nuclear changes.", "PMID": 970927}
{"id": "pubmed23n0032_8250", "title": "Comparative sensitivities of flame atomic absorption spectrophotometry (AAS) and molecular absorption spectrophotometry (MAS).", "content": "A sensitivity comparison was made between conventional atomic absorption spectrophotometry (AAS) and conventional molecular absorption spectrophotometry (MAS) for the serum trace metals, copper, iron, and zinc. The sensitivity aspect considered was absorbances was obtained for concentrations in the solutions analyzed using unit lightpaths of 1 cm and 10 cm for MAS and AAS, respectively. A distinction was made between procedural sensitivity and measurement sensitivity where the latter represents molar absorptivity. Some  was given to the concepts of procedural sensitization by concentration of the analyte through the use of lyophilization, extraction or ashing. The misuse of data obtained with scale expanders is described as a commonly occurring phenomenon of both AAS and MAS and a source of confusion in the understanding of measurement sensitivity.", "contents": "Comparative sensitivities of flame atomic absorption spectrophotometry (AAS) and molecular absorption spectrophotometry (MAS). A sensitivity comparison was made between conventional atomic absorption spectrophotometry (AAS) and conventional molecular absorption spectrophotometry (MAS) for the serum trace metals, copper, iron, and zinc. The sensitivity aspect considered was absorbances was obtained for concentrations in the solutions analyzed using unit lightpaths of 1 cm and 10 cm for MAS and AAS, respectively. A distinction was made between procedural sensitivity and measurement sensitivity where the latter represents molar absorptivity. Some  was given to the concepts of procedural sensitization by concentration of the analyte through the use of lyophilization, extraction or ashing. The misuse of data obtained with scale expanders is described as a commonly occurring phenomenon of both AAS and MAS and a source of confusion in the understanding of measurement sensitivity.", "PMID": 970929}
{"id": "pubmed23n0032_8251", "title": "Methionine overproduction by Saccharomycopsis lipolytica.", "content": "Six ethionine-resistant (Etr) regulatory mutants of Saccharomycopsis lipolytica Sl/1 overproducing methionine have been isolated. Five of them are also resistant to seleno-methionine. The activity of homocysteine synthase (O-acetyl-L-hormoserine-acetate lyase, adding hydrogen sulfide) is derepressed in these mutants and is not susceptible to the methionine-mediated repression. The pool of free methionine in Etr mutants is enhanced 1.5 to 18 times, and incorporation of 35S into methionine is 1.5 to 50 times higher than that in the wild strain. Neither accumulation of endogenous free methionine in Etr mutants nor the uptake of exogenous methionine is accompanied by an increase in the S-adenosylmethionine pool. This implies compartmentation of methionine metabolism in S. lipolytica.", "contents": "Methionine overproduction by Saccharomycopsis lipolytica. Six ethionine-resistant (Etr) regulatory mutants of Saccharomycopsis lipolytica Sl/1 overproducing methionine have been isolated. Five of them are also resistant to seleno-methionine. The activity of homocysteine synthase (O-acetyl-L-hormoserine-acetate lyase, adding hydrogen sulfide) is derepressed in these mutants and is not susceptible to the methionine-mediated repression. The pool of free methionine in Etr mutants is enhanced 1.5 to 18 times, and incorporation of 35S into methionine is 1.5 to 50 times higher than that in the wild strain. Neither accumulation of endogenous free methionine in Etr mutants nor the uptake of exogenous methionine is accompanied by an increase in the S-adenosylmethionine pool. This implies compartmentation of methionine metabolism in S. lipolytica.", "PMID": 970934}
{"id": "pubmed23n0032_8252", "title": "Study of plating efficiency of bacteriophages of thermophilic lactic acid bacteria on different media.", "content": "The qualitative and quantitative abilities of phages of 13 strains of thermophilic lactic bacteria (lactobacilli and streptococci) to produce plaques on six different media were studied. The influence of the addition of calcium on the one hand, and of incubation conditions (aero-anaerobiosis), on the other hand, was also examined. For the phages of lactobacilli, the best results were obtained with the medium of de Man, Rogosa, and Sharpe (1960), whereas for the phages of streptococci the medium of Hogg and Jago (1970) appeared to be the best. Calcium and incubation conditions play a role which is variable in importance, but rarely negligible.", "contents": "Study of plating efficiency of bacteriophages of thermophilic lactic acid bacteria on different media. The qualitative and quantitative abilities of phages of 13 strains of thermophilic lactic bacteria (lactobacilli and streptococci) to produce plaques on six different media were studied. The influence of the addition of calcium on the one hand, and of incubation conditions (aero-anaerobiosis), on the other hand, was also examined. For the phages of lactobacilli, the best results were obtained with the medium of de Man, Rogosa, and Sharpe (1960), whereas for the phages of streptococci the medium of Hogg and Jago (1970) appeared to be the best. Calcium and incubation conditions play a role which is variable in importance, but rarely negligible.", "PMID": 970935}
{"id": "pubmed23n0032_8253", "title": "Adsorption of bacteria to roots as related to host specificity in the Rhizobium-clover symbiosis.", "content": "Quantitative microscope techniques were utilized to examine the adsorption of rhizobial cells to clover root hairs. Adsorption of cells of noninfective strains of Rhizobium trifolii or infective R. meliloti strains to clover root hairs was four to five times less than that of the infective R. trifolii strains. Attachment of the rod-shaped bacteria to clover root cells occurred in a polar, end-on fashion. Viable or heat-killed R. trifolii cells precoated with a clover lectin having 2-deoxyglucose specificity had increased adsorption to clover roots. Adsorption of bacteria to roots was not increased if the clover lectin was inactivated by heat or 2-deoxyglucose treatment prior to incubation with R. trifolii. Adsorption of R. trifolii to clover root hairs was inhibited by 2-deoxyglucose (30 mM) but not by 2-deoxygalactose or alpha-D-glucose. Adsorption of R. meliloti cells to alfalfa root hairs was not affected by 2-deoxyglucose at that concentration. These results suggest that expression of host specificity in the Rhizobium-clover symbiosis involves a preferential adsorption of infective cells to clover root hairs through a 2-deoxyglucose-sensitive receptor site.", "contents": "Adsorption of bacteria to roots as related to host specificity in the Rhizobium-clover symbiosis. Quantitative microscope techniques were utilized to examine the adsorption of rhizobial cells to clover root hairs. Adsorption of cells of noninfective strains of Rhizobium trifolii or infective R. meliloti strains to clover root hairs was four to five times less than that of the infective R. trifolii strains. Attachment of the rod-shaped bacteria to clover root cells occurred in a polar, end-on fashion. Viable or heat-killed R. trifolii cells precoated with a clover lectin having 2-deoxyglucose specificity had increased adsorption to clover roots. Adsorption of bacteria to roots was not increased if the clover lectin was inactivated by heat or 2-deoxyglucose treatment prior to incubation with R. trifolii. Adsorption of R. trifolii to clover root hairs was inhibited by 2-deoxyglucose (30 mM) but not by 2-deoxygalactose or alpha-D-glucose. Adsorption of R. meliloti cells to alfalfa root hairs was not affected by 2-deoxyglucose at that concentration. These results suggest that expression of host specificity in the Rhizobium-clover symbiosis involves a preferential adsorption of infective cells to clover root hairs through a 2-deoxyglucose-sensitive receptor site.", "PMID": 970936}
{"id": "pubmed23n0032_8254", "title": "Fractionation of serum cholesterol: a critique.", "content": "A procedure for fractionation of serum cholesterol is developed by employing digitonin for the precipitation of free cholesterol in isopropanol. This method is based on the ferric acetate color reaction which is unaffected by traces of digitonin. It is influenced only negligibly by the presence of stanols in serum and is shown to be equally chromogenic with respect to both free and ester forms of cholesterol. The proposed procedure shows a three-fold improvement in precision (3.1 percent coefficient of variation [CV]) compared to that of the procedure by Leffler and McDougald (10.5 percent C.V.) which is based on the ferric chloride color reaction. The proposed free cholestrol procedure showed a mean recovery of 99.5% percent (98.1 to 102.5 percent) when cholesterol in 40 to 200 mg per dl concentrations was added to serum. The analytical performance of the proposed fractionation of serum cholesterol is critically reviewed with respect to its potential application in the diagnosis of liver diseases and in basic or experimental research.", "contents": "Fractionation of serum cholesterol: a critique. A procedure for fractionation of serum cholesterol is developed by employing digitonin for the precipitation of free cholesterol in isopropanol. This method is based on the ferric acetate color reaction which is unaffected by traces of digitonin. It is influenced only negligibly by the presence of stanols in serum and is shown to be equally chromogenic with respect to both free and ester forms of cholesterol. The proposed procedure shows a three-fold improvement in precision (3.1 percent coefficient of variation [CV]) compared to that of the procedure by Leffler and McDougald (10.5 percent C.V.) which is based on the ferric chloride color reaction. The proposed free cholestrol procedure showed a mean recovery of 99.5% percent (98.1 to 102.5 percent) when cholesterol in 40 to 200 mg per dl concentrations was added to serum. The analytical performance of the proposed fractionation of serum cholesterol is critically reviewed with respect to its potential application in the diagnosis of liver diseases and in basic or experimental research.", "PMID": 970928}
{"id": "pubmed23n0032_8255", "title": "Ring hydroxylation of p-chlorophenylacetate by an arthrobacter strain.", "content": "Resting cell suspensions of a strain of Arthrobacter grown on phenylacetate converted p-chlorophenylacetate to two products. One of the products was identified as 4-chloro-3-hydroxyphenylacetate.", "contents": "Ring hydroxylation of p-chlorophenylacetate by an arthrobacter strain. Resting cell suspensions of a strain of Arthrobacter grown on phenylacetate converted p-chlorophenylacetate to two products. One of the products was identified as 4-chloro-3-hydroxyphenylacetate.", "PMID": 970937}
{"id": "pubmed23n0032_8256", "title": "The carcinogenic effect of inhaled asbestos fibers.", "content": "Inhalation of asbestos fibers is associated with high incidence of lung cancers and pleural or peritoneal mesotheliomas in humans. All of these lesions were successfully reproduced in animal experiment, and it was shown that asbestos neoplasm may occur with or without accompanying asbestosis. Incidence of tumors from crocidolite was nearly three times as high as from chrysotile or amosite. It is possible that different carcinogenic entities are responsible for the causation of lung tumors and mesothelial tumors. Lung tumors seem to depend on the adsorptive capacity of asbestos fibers, allowing other carcinogens (heavy metals, polycyclic hydrocarbons, cigarette smoke) to attain a critical focal concentration. Mesothelial tumors, on the other hand, might arise in response to mechanical irritation by fibers which may become lodged during lymphatic spread. Tissues subject to constant respiratory movement (e.g., pleura or peritoneum) are specifically vulnerable to the latter action.", "contents": "The carcinogenic effect of inhaled asbestos fibers. Inhalation of asbestos fibers is associated with high incidence of lung cancers and pleural or peritoneal mesotheliomas in humans. All of these lesions were successfully reproduced in animal experiment, and it was shown that asbestos neoplasm may occur with or without accompanying asbestosis. Incidence of tumors from crocidolite was nearly three times as high as from chrysotile or amosite. It is possible that different carcinogenic entities are responsible for the causation of lung tumors and mesothelial tumors. Lung tumors seem to depend on the adsorptive capacity of asbestos fibers, allowing other carcinogens (heavy metals, polycyclic hydrocarbons, cigarette smoke) to attain a critical focal concentration. Mesothelial tumors, on the other hand, might arise in response to mechanical irritation by fibers which may become lodged during lymphatic spread. Tissues subject to constant respiratory movement (e.g., pleura or peritoneum) are specifically vulnerable to the latter action.", "PMID": 970931}
{"id": "pubmed23n0032_8257", "title": "Preparation of phase 1Q fever antigen suitable for vaccine use.", "content": "Five lots of phase 1 Q fever antigen suitable for use as vaccine were prepared utilizing the Freon-brushite method of antigen purification. Antigen concentration levels were initially adjusted turbidimetrically and later confirmed by antibody response in guinea pigs. Data showed that all lots were essentially identical in purity and antigenicity.", "contents": "Preparation of phase 1Q fever antigen suitable for vaccine use. Five lots of phase 1 Q fever antigen suitable for use as vaccine were prepared utilizing the Freon-brushite method of antigen purification. Antigen concentration levels were initially adjusted turbidimetrically and later confirmed by antibody response in guinea pigs. Data showed that all lots were essentially identical in purity and antigenicity.", "PMID": 970938}
{"id": "pubmed23n0032_8258", "title": "Carbon monoxide in firemen before and after exposure to smoke.", "content": "A statistically significant difference was found between the mean baseline carboxyhemoglobin of non-smoking firemen (COHb 2.53, sd = 1.05, n = 32) and smoking firemen (COHb 5.54, sd = 2.61, n = 26). A consistent increase in mean COHb levels after exposure to smoke was seen in both non-smoking (4.41 sd = 2.95, n = 7) and smoking men (8.74, sd = 5.66, n = 11), but the mean increase in these two groups was statistically significant only at the 90 percent level (t = 1.85, df = 16, p less than 0.1).", "contents": "Carbon monoxide in firemen before and after exposure to smoke. A statistically significant difference was found between the mean baseline carboxyhemoglobin of non-smoking firemen (COHb 2.53, sd = 1.05, n = 32) and smoking firemen (COHb 5.54, sd = 2.61, n = 26). A consistent increase in mean COHb levels after exposure to smoke was seen in both non-smoking (4.41 sd = 2.95, n = 7) and smoking men (8.74, sd = 5.66, n = 11), but the mean increase in these two groups was statistically significant only at the 90 percent level (t = 1.85, df = 16, p less than 0.1).", "PMID": 970930}
{"id": "pubmed23n0032_8259", "title": "Effect of dissolved aromatic hydrocarbons on the growth of marine bacteria in batch culture.", "content": "Dissolved aromatic hydrocarbons were found to decrease growth rate and maximum cell density of marine bacteria in batch cultures. The magnitude of the decrement was observed to be a function of concentration of the hydrocarbon and inherent toxicity. The inherent toxicity was observed to increase inversely with solubility such that naphthalene at 100 muM concentration demonstrated a toxic effect similar to benzopyrene at 0.02 muM. A partial oxidation product of naphthalene was found to be more effective in decreasing growth parameters than naphthalene at equivalent concentrations and to cause complete cessation of growth at the higher concentrations permitted by its polar structure.", "contents": "Effect of dissolved aromatic hydrocarbons on the growth of marine bacteria in batch culture. Dissolved aromatic hydrocarbons were found to decrease growth rate and maximum cell density of marine bacteria in batch cultures. The magnitude of the decrement was observed to be a function of concentration of the hydrocarbon and inherent toxicity. The inherent toxicity was observed to increase inversely with solubility such that naphthalene at 100 muM concentration demonstrated a toxic effect similar to benzopyrene at 0.02 muM. A partial oxidation product of naphthalene was found to be more effective in decreasing growth parameters than naphthalene at equivalent concentrations and to cause complete cessation of growth at the higher concentrations permitted by its polar structure.", "PMID": 970939}
{"id": "pubmed23n0032_8260", "title": "Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media.", "content": "Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of sterigmatocystin by B. sorokiniana was negligible, whereas maximal production by A. versicolor was 210 mg/liter. On solid substrates, the highest yields (8 g/kg) were obtained with A. versicolor on still cultures of whole corn supplemented with Soytone.", "contents": "Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media. Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of sterigmatocystin by B. sorokiniana was negligible, whereas maximal production by A. versicolor was 210 mg/liter. On solid substrates, the highest yields (8 g/kg) were obtained with A. versicolor on still cultures of whole corn supplemented with Soytone.", "PMID": 970940}
{"id": "pubmed23n0032_8261", "title": "Metabolism of DDT analogues by a Pseudomonas sp.", "content": "A Pseudomonas sp. rapidly metabolized several nonchlorinated analogues of DDT, with the exception of 2,2-diphenylethanol, as the sole carbon source. Several of the mono-p-chloro-substituted diphenyl analogues were also metabolized as the sole carbon source by the bacterium. The resulting chlorinated aromatic acid metabolites were not further metabolized. The isolate was unable to metabolize p,p'-dichlorodiphenyl analogues as the sole carbon source.", "contents": "Metabolism of DDT analogues by a Pseudomonas sp. A Pseudomonas sp. rapidly metabolized several nonchlorinated analogues of DDT, with the exception of 2,2-diphenylethanol, as the sole carbon source. Several of the mono-p-chloro-substituted diphenyl analogues were also metabolized as the sole carbon source by the bacterium. The resulting chlorinated aromatic acid metabolites were not further metabolized. The isolate was unable to metabolize p,p'-dichlorodiphenyl analogues as the sole carbon source.", "PMID": 970941}
{"id": "pubmed23n0032_8262", "title": "Volatiles produced by microorganisms isolated from refrigerated chicken at spoilage.", "content": "Volatile components present at spoilage of refrigerated chicken breasts were identified using high-vacuum-low-temperature distillation techniques followed by analysis with combined temperature-programmed gas chromatography and mass spectrometry. A comparison was made of the compounds detected from both irradiated and non-irradiated muscle stored at 2 and 10 degrees C under both aerobic and anaerobic conditions. Isolates were randomly selected from the spoiled poultry, identified, and evaluated for their ability to produce volatile spoilage noted when grown on radiation-sterilized chicken. Several isolates that produced off-odors on sterile chicken breasts were examined. Twenty-two compounds were associated with spoilage. Some of the compounds found on both irradiated and unirradiated samples were considered to play only a minor role in the spoilage aroma or were present in low concentrations, since the aroma of spoiled irradiated chicken lacked the harsh odor notes typical of spoiled unirradiated chicken. Fifteen of the 22 compounds were considered to be unique to unirradiated, aerobically spoiled samples. Nine of these compounds, hydrogen sulfide, methyl mercaptan, dimethyl sulfide, dimethyl disulfide, methyl acetate, ethyl acetate, heptadiene, methanol, and ethanol, were found on chicken spoiled at both 2 and 10 degrees C. xylene, benzaldehyde, and 2,3-dithiahexane were detected only in samples stored at 2 degrees C and methyl thiolacetate, 2-butanone, and ethyl propionate were associated with 10 degrees C spoilage. Fifty-eight isolates randomly selected from fresh, radiation-pasteurized, and unirradiated spoiled poultry were classified taxonomically, and 10 of them, which produced spoilage odors on sterilized chicken breasts, were selected for subsequent analysis of their volatiles. Isolates identified as Pseudomonas putrefaciens and Pseudomonas species that were members of groups I and II of Shewan's classification, as well as Flavobacterium and oxidative Moraxella, produced a number of the compounds found in the aroma of spoiled chicken. A total of 17 compounds were identified. Whereas no isolate produced all of the aroma compounds found in the aroma of spoiled chicken, together they did produce the nine found in unirradiated samples spoiled at either 2 or 10 degrees C, as well as methyl thiolacetate and xylene. Six compounds were present in the volatiles produced by the isolates but were absent in the volatiles identified from spoiled chicken. These were hydrogen cyanide, methyl isopropyl sulfide, 2-propane thiol, methyl propionate, ethyl benzene, and an unidentified compound.", "contents": "Volatiles produced by microorganisms isolated from refrigerated chicken at spoilage. Volatile components present at spoilage of refrigerated chicken breasts were identified using high-vacuum-low-temperature distillation techniques followed by analysis with combined temperature-programmed gas chromatography and mass spectrometry. A comparison was made of the compounds detected from both irradiated and non-irradiated muscle stored at 2 and 10 degrees C under both aerobic and anaerobic conditions. Isolates were randomly selected from the spoiled poultry, identified, and evaluated for their ability to produce volatile spoilage noted when grown on radiation-sterilized chicken. Several isolates that produced off-odors on sterile chicken breasts were examined. Twenty-two compounds were associated with spoilage. Some of the compounds found on both irradiated and unirradiated samples were considered to play only a minor role in the spoilage aroma or were present in low concentrations, since the aroma of spoiled irradiated chicken lacked the harsh odor notes typical of spoiled unirradiated chicken. Fifteen of the 22 compounds were considered to be unique to unirradiated, aerobically spoiled samples. Nine of these compounds, hydrogen sulfide, methyl mercaptan, dimethyl sulfide, dimethyl disulfide, methyl acetate, ethyl acetate, heptadiene, methanol, and ethanol, were found on chicken spoiled at both 2 and 10 degrees C. xylene, benzaldehyde, and 2,3-dithiahexane were detected only in samples stored at 2 degrees C and methyl thiolacetate, 2-butanone, and ethyl propionate were associated with 10 degrees C spoilage. Fifty-eight isolates randomly selected from fresh, radiation-pasteurized, and unirradiated spoiled poultry were classified taxonomically, and 10 of them, which produced spoilage odors on sterilized chicken breasts, were selected for subsequent analysis of their volatiles. Isolates identified as Pseudomonas putrefaciens and Pseudomonas species that were members of groups I and II of Shewan's classification, as well as Flavobacterium and oxidative Moraxella, produced a number of the compounds found in the aroma of spoiled chicken. A total of 17 compounds were identified. Whereas no isolate produced all of the aroma compounds found in the aroma of spoiled chicken, together they did produce the nine found in unirradiated samples spoiled at either 2 or 10 degrees C, as well as methyl thiolacetate and xylene. Six compounds were present in the volatiles produced by the isolates but were absent in the volatiles identified from spoiled chicken. These were hydrogen cyanide, methyl isopropyl sulfide, 2-propane thiol, methyl propionate, ethyl benzene, and an unidentified compound.", "PMID": 970942}
{"id": "pubmed23n0032_8263", "title": "Effects of nutritional characteristics of Streptococcus agalactiae on inhibition of growth by lactoperoxidase-thiocyanate-hydrogen peroxide in chemically defined culture medium.", "content": "Five cultures of Streptococcus agalactiae have an absolute requirement for L-cystine to grow in a chemically defined medium. The L-cystine could be replaced with cysteine, glutathione, or the disulfide form of glutathione. Dithiothreitol could not substitute for the sulfur-containing amino acids of glutathione; hence, the growth requirement appears to be truly nutritional. Growth was maximum with 4 to 5 mug of L-cystine per ml. If the concentration of L-cystine was no greater than 4 to 5 mug/ml, complete growth inhibition could be obtained by the addition of lactoperoxidase, thiocyanate, and H2O2. The growth inhibition, however, was nullified by additions of L-cystine 10-fold or more in excess of the concentration needed for maximum growth. During the aerobic degradation of glucose by cell suspensions, H2O2 accumulation could be shown with cultures 317 and 11-13, the only cultures the growth of which was inhibited without addition of exogenous H2O2. All of the cultures had varying degrees of peroxidase activity. The balance between H2O2 generation and peroxidase activity of the culture evidently determined whether growth could be inhibited with lactoperoxidase and thiocyanate without H2O2 addition. The growth yeilds per 0.5 mol of the disulfide forms (cystine and oxidized glutathione) were 1.5 and 1.9 times greater than that per 1 mol of the sulfhydryl forms (cysteine and glutathione).", "contents": "Effects of nutritional characteristics of Streptococcus agalactiae on inhibition of growth by lactoperoxidase-thiocyanate-hydrogen peroxide in chemically defined culture medium. Five cultures of Streptococcus agalactiae have an absolute requirement for L-cystine to grow in a chemically defined medium. The L-cystine could be replaced with cysteine, glutathione, or the disulfide form of glutathione. Dithiothreitol could not substitute for the sulfur-containing amino acids of glutathione; hence, the growth requirement appears to be truly nutritional. Growth was maximum with 4 to 5 mug of L-cystine per ml. If the concentration of L-cystine was no greater than 4 to 5 mug/ml, complete growth inhibition could be obtained by the addition of lactoperoxidase, thiocyanate, and H2O2. The growth inhibition, however, was nullified by additions of L-cystine 10-fold or more in excess of the concentration needed for maximum growth. During the aerobic degradation of glucose by cell suspensions, H2O2 accumulation could be shown with cultures 317 and 11-13, the only cultures the growth of which was inhibited without addition of exogenous H2O2. All of the cultures had varying degrees of peroxidase activity. The balance between H2O2 generation and peroxidase activity of the culture evidently determined whether growth could be inhibited with lactoperoxidase and thiocyanate without H2O2 addition. The growth yeilds per 0.5 mol of the disulfide forms (cystine and oxidized glutathione) were 1.5 and 1.9 times greater than that per 1 mol of the sulfhydryl forms (cysteine and glutathione).", "PMID": 970943}
{"id": "pubmed23n0032_8264", "title": "Effect of storage time and temperature and the variation among replicate tests (on different days) on the performance of spore disks and strips.", "content": "Laboratory-prepared spore disks were stored for 96 weeks at 22 degrees C with 50% relative humidity (RH) and at 4 degrees C with less than 1% RH. At the same time commercial spore strips were stored for 64 weeks at 22 degrees C with 50% RH. The spore count per unit and the heat resistance were measured at the beginning of the experiment and after 16, 32, 48, 64, 80, and 96 weeks of storage. The laboratory-prepared spore disks stored at 4 degrees C with less than 1% RH showed less change in numbers of spores per disks and decrease in the survival time than did the disks stored at 22 degrees C with 50% RH. Both the laboratory-prepared spore disks and  the commercial spore strips stored at 22 degrees C with 50% RH decreased in survival times with increased storage time. The relative change in the survival times with storage was less for the commercial spore strips than for the laboratory-prepared spore disks.", "contents": "Effect of storage time and temperature and the variation among replicate tests (on different days) on the performance of spore disks and strips. Laboratory-prepared spore disks were stored for 96 weeks at 22 degrees C with 50% relative humidity (RH) and at 4 degrees C with less than 1% RH. At the same time commercial spore strips were stored for 64 weeks at 22 degrees C with 50% RH. The spore count per unit and the heat resistance were measured at the beginning of the experiment and after 16, 32, 48, 64, 80, and 96 weeks of storage. The laboratory-prepared spore disks stored at 4 degrees C with less than 1% RH showed less change in numbers of spores per disks and decrease in the survival time than did the disks stored at 22 degrees C with 50% RH. Both the laboratory-prepared spore disks and  the commercial spore strips stored at 22 degrees C with 50% RH decreased in survival times with increased storage time. The relative change in the survival times with storage was less for the commercial spore strips than for the laboratory-prepared spore disks.", "PMID": 970944}
{"id": "pubmed23n0032_8265", "title": "Inhibition of methanogenesis in salt marsh sediments and whole-cell suspensions of methanogenic bacteria by nitrogen oxides.", "content": "Hydrogen-dependent evolution of methane from salt marsh sediments and whole-cell suspensions of Methanobacterium thermoautotrophicum and Methanobacterium fornicicum ceased or decreased after the introduction of nitrate, nitrite, nitric oxide, or nitrous oxide. Sulfite had a similar effect on methanogenesis in the whole-cell suspensions. In salt marsh sediments, nitrous oxide was the strongest inhibitor, followed by nitric oxide, nitrite, and nitrate in decreasing order of inhibition. In whole-cell suspensions, nitric oxide was the strongest inhibitor, followed by nitrous oxide, nitrite, and nitrate. Consideration of the results from experiments using an indicator of oxidation potential, along with the reversed order of effectiveness of the nitrogen oxides in relation to their degree of reduction ,suggests that the inhibitory effect observed was not due to a redox change. Evidence is also presented that suggests that the decrease in the rate of methane production in the presence of oxides of nitrogen was not attributable to competition for methane-producing substrates.", "contents": "Inhibition of methanogenesis in salt marsh sediments and whole-cell suspensions of methanogenic bacteria by nitrogen oxides. Hydrogen-dependent evolution of methane from salt marsh sediments and whole-cell suspensions of Methanobacterium thermoautotrophicum and Methanobacterium fornicicum ceased or decreased after the introduction of nitrate, nitrite, nitric oxide, or nitrous oxide. Sulfite had a similar effect on methanogenesis in the whole-cell suspensions. In salt marsh sediments, nitrous oxide was the strongest inhibitor, followed by nitric oxide, nitrite, and nitrate in decreasing order of inhibition. In whole-cell suspensions, nitric oxide was the strongest inhibitor, followed by nitrous oxide, nitrite, and nitrate. Consideration of the results from experiments using an indicator of oxidation potential, along with the reversed order of effectiveness of the nitrogen oxides in relation to their degree of reduction ,suggests that the inhibitory effect observed was not due to a redox change. Evidence is also presented that suggests that the decrease in the rate of methane production in the presence of oxides of nitrogen was not attributable to competition for methane-producing substrates.", "PMID": 970945}
{"id": "pubmed23n0032_8266", "title": "Metabolism and growth yields in Bacteroides ruminicola strain b14.", "content": "Metabolism of D-glucose by Bacteroides ruminicola subsp. brevis, strain B14, has been examined. Growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately 66 g (dry weight)/mol of glucose fermented. The storage polysaccharide amounted to about 14% of the total dry weight, or 55% of the total cellular carbohydrate, at full growth. After correcting glucose utilization for incorporation into cellular carbohydrate, measurement of product formation showed that 1.1 succinate, 0.8 acetate, and 0.35 formate are produced and 0.5 CO2 net is taken up during the fermentation of 1 glucose under the conditions used. The implication of these results with respect to adenosine 5'-triphosphate (ATP) molar growth yield calculations is discussed. If substrate-level phosphorylation reactions alone are responsible for ATP generation, then the ATP molar growth yield must be about 23 g (dry weight)/mol of ATP. Alternatively, if anaerobic electron transfer-linked phosphorylation also occurs, the ATP molar growth yield will be lower.", "contents": "Metabolism and growth yields in Bacteroides ruminicola strain b14. Metabolism of D-glucose by Bacteroides ruminicola subsp. brevis, strain B14, has been examined. Growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately 66 g (dry weight)/mol of glucose fermented. The storage polysaccharide amounted to about 14% of the total dry weight, or 55% of the total cellular carbohydrate, at full growth. After correcting glucose utilization for incorporation into cellular carbohydrate, measurement of product formation showed that 1.1 succinate, 0.8 acetate, and 0.35 formate are produced and 0.5 CO2 net is taken up during the fermentation of 1 glucose under the conditions used. The implication of these results with respect to adenosine 5'-triphosphate (ATP) molar growth yield calculations is discussed. If substrate-level phosphorylation reactions alone are responsible for ATP generation, then the ATP molar growth yield must be about 23 g (dry weight)/mol of ATP. Alternatively, if anaerobic electron transfer-linked phosphorylation also occurs, the ATP molar growth yield will be lower.", "PMID": 970946}
{"id": "pubmed23n0032_8267", "title": "Preparation of pure microbiological samples for pyrolysis of gas-liquid chromatography studies.", "content": "Bacterial samples were prepared for pyrolysis gas-liquid chromatography using cells grown on membrane filters. Pyrochromatograms were reproducible when cells harvested from the filters were pyrolyzed without being washed.", "contents": "Preparation of pure microbiological samples for pyrolysis of gas-liquid chromatography studies. Bacterial samples were prepared for pyrolysis gas-liquid chromatography using cells grown on membrane filters. Pyrochromatograms were reproducible when cells harvested from the filters were pyrolyzed without being washed.", "PMID": 970947}
{"id": "pubmed23n0032_8268", "title": "Fractures in Paget's disease of bone.", "content": "After a short introduction dealing with the orthopaedic problems of Paget's disease, six patients are reported with pathological fractures due to this disease, which is rare in The Netherlands. Rigid fixation with osteosynthesis is the treatment of choice. In cases in which substantial bowing of the bone exists, a corrective osteotomy is advisable.", "contents": "Fractures in Paget's disease of bone. After a short introduction dealing with the orthopaedic problems of Paget's disease, six patients are reported with pathological fractures due to this disease, which is rare in The Netherlands. Rigid fixation with osteosynthesis is the treatment of choice. In cases in which substantial bowing of the bone exists, a corrective osteotomy is advisable.", "PMID": 970968}
{"id": "pubmed23n0032_8269", "title": "Discoid knee menisci in children.", "content": "Five children were operated for discoid menisci. One of them had, in addition, an anomalous capsular attachment and an anomalous course of the popliteus tendon. Vascularisation at the free margin was a striking feature in the four discoid menisci of the primitive type. Limited or painful extension was common in these children. In four cases the diagnosis was confirmed arthrographically. Arthrography was carried out in two patients respectively four and sixteen years old, after operation. In both cases a small regenerated meniscus was found as is seen after meniscectomy in adults.", "contents": "Discoid knee menisci in children. Five children were operated for discoid menisci. One of them had, in addition, an anomalous capsular attachment and an anomalous course of the popliteus tendon. Vascularisation at the free margin was a striking feature in the four discoid menisci of the primitive type. Limited or painful extension was common in these children. In four cases the diagnosis was confirmed arthrographically. Arthrography was carried out in two patients respectively four and sixteen years old, after operation. In both cases a small regenerated meniscus was found as is seen after meniscectomy in adults.", "PMID": 970969}
{"id": "pubmed23n0032_8270", "title": "Use of the lag screw for the surgical treatment of subcapital fractures of the femoral neck with slight displacement.", "content": "A report is given of the results of surgical treatment with a compression screw in a group of 22 patients with subcapital fractures of the femoral neck with minor displacement. The patients' average age was 63 years. Postoperatively, two patients (aged 75 and 83) died from complications not directly attributable to the operation. The remaining 20 patients were followed-up for two to eight years by means of personal interview, functional studies and radiological control. Two of the 20 patients died from unrelated causes at an early stage of the survey; however, the condition of the fractures at the time of death was satisfactory. Necrosis of the femoral head occurred in two cases. In all, a good functional result was obtained in 18 patients (90%). It is pointed out that these patients constituted a selected group, in which the prognosis was favourable in any case.", "contents": "Use of the lag screw for the surgical treatment of subcapital fractures of the femoral neck with slight displacement. A report is given of the results of surgical treatment with a compression screw in a group of 22 patients with subcapital fractures of the femoral neck with minor displacement. The patients' average age was 63 years. Postoperatively, two patients (aged 75 and 83) died from complications not directly attributable to the operation. The remaining 20 patients were followed-up for two to eight years by means of personal interview, functional studies and radiological control. Two of the 20 patients died from unrelated causes at an early stage of the survey; however, the condition of the fractures at the time of death was satisfactory. Necrosis of the femoral head occurred in two cases. In all, a good functional result was obtained in 18 patients (90%). It is pointed out that these patients constituted a selected group, in which the prognosis was favourable in any case.", "PMID": 970970}
{"id": "pubmed23n0032_8271", "title": "The Christiansen hip prosthesis preliminary results.", "content": "The combined endo- and total hip prosthesis of Christiansen is described. In this prosthesis an articular connection, or trunnion bearing, is introduced between the head and stem components in order to reduce friction at the acetabular joint. The stem portions of both prostheses are identical. The endoprosthesis was inserted in 50 patients, who were followed-up during one year. The results were according to the classification of Love (Surg. Clin. N. Amer., 1963, 43, p. 1217): excellent in 60%, good in 22%, and poor in 18% of cases. In addition some results from other Norwegian hospitals were presented.", "contents": "The Christiansen hip prosthesis preliminary results. The combined endo- and total hip prosthesis of Christiansen is described. In this prosthesis an articular connection, or trunnion bearing, is introduced between the head and stem components in order to reduce friction at the acetabular joint. The stem portions of both prostheses are identical. The endoprosthesis was inserted in 50 patients, who were followed-up during one year. The results were according to the classification of Love (Surg. Clin. N. Amer., 1963, 43, p. 1217): excellent in 60%, good in 22%, and poor in 18% of cases. In addition some results from other Norwegian hospitals were presented.", "PMID": 970971}
{"id": "pubmed23n0032_8272", "title": "Histoplasmoma of the lung.", "content": "A review of all granulomatous surgical lesions in the files of the Free University Hospital revealed four cases of histoplasmoma in which Grocott-staining procedures provided histologic confirmation of the presence of Histoplasma capsulatum bodies. The case histories have been presented briefly. A short review of the literature follows.", "contents": "Histoplasmoma of the lung. A review of all granulomatous surgical lesions in the files of the Free University Hospital revealed four cases of histoplasmoma in which Grocott-staining procedures provided histologic confirmation of the presence of Histoplasma capsulatum bodies. The case histories have been presented briefly. A short review of the literature follows.", "PMID": 970972}
{"id": "pubmed23n0032_8273", "title": "Isolated and atypical manifestations of Scheuermann's disease.", "content": "In addition to the typical form of Scheuermann's disease, atypical and localized manifestations also occur. Examples are paradiscal defects and the development of vertebral synostosis. These unusual manifestations are illustrated by reference to a number of patients.", "contents": "Isolated and atypical manifestations of Scheuermann's disease. In addition to the typical form of Scheuermann's disease, atypical and localized manifestations also occur. Examples are paradiscal defects and the development of vertebral synostosis. These unusual manifestations are illustrated by reference to a number of patients.", "PMID": 970973}
{"id": "pubmed23n0032_8274", "title": "Perforating rectal injuries.", "content": "The symptomatology, diagnosis and treatment of perforating rectal injuries are discussed. An analysis is presented of the data concerning 24 patients with intra- and extraperitoneal penetration of the rectum. Factors that contribute to the continuing high mortality rate of rectal perforation are its rarity, the highly infectious character of faecal contamination of the abdominal cavity or perirectal tissue, and the fact that examination often reveals little or no external trauma. The treatment of choice would appear to be an aggressive approach based on faecal diversion and drainage, supplemented with peroperative irrigation of the injured rectum.", "contents": "Perforating rectal injuries. The symptomatology, diagnosis and treatment of perforating rectal injuries are discussed. An analysis is presented of the data concerning 24 patients with intra- and extraperitoneal penetration of the rectum. Factors that contribute to the continuing high mortality rate of rectal perforation are its rarity, the highly infectious character of faecal contamination of the abdominal cavity or perirectal tissue, and the fact that examination often reveals little or no external trauma. The treatment of choice would appear to be an aggressive approach based on faecal diversion and drainage, supplemented with peroperative irrigation of the injured rectum.", "PMID": 970974}
{"id": "pubmed23n0032_8275", "title": "Findings in patients with diverticulosis and diverticulitis of the colon.", "content": "During the period from 1957 up to and including December 1975, the surgical clinic of Nijmegen admitted 82 patients with the diagnoses of diverticulosis and diverticulitis of the colon: the diagnosis of diverticulosis was made in 16 cases; in 17 diverticulitis was diagnosed and these patients were treated by conservative methods. Forty-nine patients were subjected to operation for diverticulitis. Of the patients treated conservatively, none died; of those treated by operation, three (6%) died. There were nine patients with peritonitis and three with ileus who required emergency surgery. As a matter of principle these patients were treated in several stages; none of them died.", "contents": "Findings in patients with diverticulosis and diverticulitis of the colon. During the period from 1957 up to and including December 1975, the surgical clinic of Nijmegen admitted 82 patients with the diagnoses of diverticulosis and diverticulitis of the colon: the diagnosis of diverticulosis was made in 16 cases; in 17 diverticulitis was diagnosed and these patients were treated by conservative methods. Forty-nine patients were subjected to operation for diverticulitis. Of the patients treated conservatively, none died; of those treated by operation, three (6%) died. There were nine patients with peritonitis and three with ileus who required emergency surgery. As a matter of principle these patients were treated in several stages; none of them died.", "PMID": 970975}
{"id": "pubmed23n0032_8276", "title": "The femoro-femoral cross-over graft.", "content": "The femoro-femoral cross-over graft is a good alternative to direct reconstructive vascular surgery in high-risk cases of unilateral iliac artery occlusion. The technique is simple and can readily be performed under local anaesthesia. A series of 21 patients is discussed briefly. Blood-flow measurements in seven patients confirmed the observation of other workers that a steal syndrome from the donor leg only occurred when the outflow tract in the donor leg was worse than in the ischaemic leg. Flow in the donor iliac artery was considerable increased. Four patients died in the observation period, one graft occluded three months after operation. There was no late failures.", "contents": "The femoro-femoral cross-over graft. The femoro-femoral cross-over graft is a good alternative to direct reconstructive vascular surgery in high-risk cases of unilateral iliac artery occlusion. The technique is simple and can readily be performed under local anaesthesia. A series of 21 patients is discussed briefly. Blood-flow measurements in seven patients confirmed the observation of other workers that a steal syndrome from the donor leg only occurred when the outflow tract in the donor leg was worse than in the ischaemic leg. Flow in the donor iliac artery was considerable increased. Four patients died in the observation period, one graft occluded three months after operation. There was no late failures.", "PMID": 970976}
{"id": "pubmed23n0032_8277", "title": "Some aspects of zygomaticomaxillary fractures.", "content": "Early diagnosis and treatment of zygomaticomaxillary fractures are essential. From results obtained in the treatment of 500 patients with fractures of the zygomaticomaxillary complex it was concluded that insertion of an implant on the orbital floor through an infra-orbital incision can be avoided. This procedure is seldom indicated, and has to be reserved for some old untreated fractures. However, it may also be indicated for young children with a blow-out fracture of the orbital floor (solitary or as part of a zygomaticomaxillary fracture). In these cases an approach through the maxillary sinus, as commonly used in adults, will result in injury or removal of the dental germs, which are still localized high in the maxilla. The possible cause of a blow-out fracture of the orbital floor (traumatic lesion of the infra-orbital margin) is discussed with reference to recent literature and to a number of patients, including a boy aged 7 1/2 years.", "contents": "Some aspects of zygomaticomaxillary fractures. Early diagnosis and treatment of zygomaticomaxillary fractures are essential. From results obtained in the treatment of 500 patients with fractures of the zygomaticomaxillary complex it was concluded that insertion of an implant on the orbital floor through an infra-orbital incision can be avoided. This procedure is seldom indicated, and has to be reserved for some old untreated fractures. However, it may also be indicated for young children with a blow-out fracture of the orbital floor (solitary or as part of a zygomaticomaxillary fracture). In these cases an approach through the maxillary sinus, as commonly used in adults, will result in injury or removal of the dental germs, which are still localized high in the maxilla. The possible cause of a blow-out fracture of the orbital floor (traumatic lesion of the infra-orbital margin) is discussed with reference to recent literature and to a number of patients, including a boy aged 7 1/2 years.", "PMID": 970977}
{"id": "pubmed23n0032_8278", "title": "Distribution of HEOD (dieldrin) in mammals: III. Transport--transfer.", "content": "A mathematical model simulating the blood transport and tissue residue distributions of the highly toxic and highly lipid soluble pesticide dieldrin in mammals is presented. This model is a significant improvement over our previously published preliminary model for dieldrin distribution in mammals. The assumptions and working hypotheses of the model are presented and used in generating a set of differential equations based upon mass balance principles. Two simulation cases are examined. The first simply demonstrates the gross features of: 1) Transport limiting conditions; 2) equal transport-equal membrane transfer conditions, and 3) membrane transfer limit conditions. The second studies a single tissue (the blood-brain barrier case) example of the above mentioned conditions. All simulations made were conducted for a hypothetical mature male rate of the average Wistar type eating food ad lib.", "contents": "Distribution of HEOD (dieldrin) in mammals: III. Transport--transfer. A mathematical model simulating the blood transport and tissue residue distributions of the highly toxic and highly lipid soluble pesticide dieldrin in mammals is presented. This model is a significant improvement over our previously published preliminary model for dieldrin distribution in mammals. The assumptions and working hypotheses of the model are presented and used in generating a set of differential equations based upon mass balance principles. Two simulation cases are examined. The first simply demonstrates the gross features of: 1) Transport limiting conditions; 2) equal transport-equal membrane transfer conditions, and 3) membrane transfer limit conditions. The second studies a single tissue (the blood-brain barrier case) example of the above mentioned conditions. All simulations made were conducted for a hypothetical mature male rate of the average Wistar type eating food ad lib.", "PMID": 970981}
{"id": "pubmed23n0032_8279", "title": "Parathion persistence on South African citrus.", "content": "The fate of parathion applied to citrus was affected by rain, sun and wind, but not much by fruit variety, while the application method had an important effect. The formulation influenced the persistence of parathion on leaves and glass in the laboratory, but had no influence on the persistence on leaves or fruit in the field. The deposits of the emulsifiable concentrate and of the wettable powder and oil mixture were higher than that of the wettable powder alone, but since the rates of disappearance were the same, higher harvest-time parathion residues on and in the fruit resulted when the first two formulations were used. The time needed to reach a tolerance value increased for applications made later in the growing season.", "contents": "Parathion persistence on South African citrus. The fate of parathion applied to citrus was affected by rain, sun and wind, but not much by fruit variety, while the application method had an important effect. The formulation influenced the persistence of parathion on leaves and glass in the laboratory, but had no influence on the persistence on leaves or fruit in the field. The deposits of the emulsifiable concentrate and of the wettable powder and oil mixture were higher than that of the wettable powder alone, but since the rates of disappearance were the same, higher harvest-time parathion residues on and in the fruit resulted when the first two formulations were used. The time needed to reach a tolerance value increased for applications made later in the growing season.", "PMID": 970982}
{"id": "pubmed23n0032_8280", "title": "The deleterious effects of fungicides and herbicides on Xenopus laevis embryos.", "content": "Groups of 30 Xenopus laevis embryos, at \"tail-bud\" stage (Nieukoop-Faber stages 22-24) were exposed to 0.1-2 ppm concentrations of various pesticides for 1 to 10 days. The pesticides used were chloranil and dichlone (both are fungicidal and herbicidal); diquat (herbicide); and nabam (fungicide). The parameters examined were mortality, gross morphology, histology, and behavior. Chloranil (1.25 to 1.75 ppm) treated embryos showed abnormalities of the otolith, optic cup, and general pigmentation. Their movement was sporadically convulsive and they were unable to maintain proper balance. Dichlone (0.1 to 0.15 ppm) disrupted the development of the cephalic end of the embryo. Many of these embryos developed a slightly retarded trunk and tail only. These headless embryos lived for a time and were relatively lethargic. Diquat (0.75 to 2.0 ppm) administration reduced body size and pigmentation, and altered body shape. When embryos were treated with both 1.0 ppm of diquat and 2.0 ppm of nabam the integrity of myomeres and myocommata of the musculature was disrupted. The histological bases of these morphological and behavioral changes are discussed.", "contents": "The deleterious effects of fungicides and herbicides on Xenopus laevis embryos. Groups of 30 Xenopus laevis embryos, at \"tail-bud\" stage (Nieukoop-Faber stages 22-24) were exposed to 0.1-2 ppm concentrations of various pesticides for 1 to 10 days. The pesticides used were chloranil and dichlone (both are fungicidal and herbicidal); diquat (herbicide); and nabam (fungicide). The parameters examined were mortality, gross morphology, histology, and behavior. Chloranil (1.25 to 1.75 ppm) treated embryos showed abnormalities of the otolith, optic cup, and general pigmentation. Their movement was sporadically convulsive and they were unable to maintain proper balance. Dichlone (0.1 to 0.15 ppm) disrupted the development of the cephalic end of the embryo. Many of these embryos developed a slightly retarded trunk and tail only. These headless embryos lived for a time and were relatively lethargic. Diquat (0.75 to 2.0 ppm) administration reduced body size and pigmentation, and altered body shape. When embryos were treated with both 1.0 ppm of diquat and 2.0 ppm of nabam the integrity of myomeres and myocommata of the musculature was disrupted. The histological bases of these morphological and behavioral changes are discussed.", "PMID": 970983}
{"id": "pubmed23n0032_8281", "title": "Degradation of compounds containing carbon atoms by photooxidation in the presence of water.", "content": "It was shown that all compounds containing carbon atoms, tested in this study, were degraded by the coincident influence of light, oxygen (air), and water. By this photooxidation, saturated as well as unsaturated aliphatic and aromatic compounds, monomeric, polymeric and high polymeric compounds were degraded and the carbon atoms of these compounds as well as carbon itself in form of amorphous carbon or diamond, were converted to carbon dioxide, if dispersed in water or together with water as very small solid, liquid, or gaseous particles. As such conditions occur extensively in nature, it was concluded that this degradation of organic substances is of great importance in nature, in addition to microbial and enzymatic degradation. This photooxidation further represents a possibility for pollution control to prevent damage to environment by degrading organic substances which are either slightly soluble or practically insoluble in water, as well as compounds which will not be destroyed by microbial and enzymatic degradation.", "contents": "Degradation of compounds containing carbon atoms by photooxidation in the presence of water. It was shown that all compounds containing carbon atoms, tested in this study, were degraded by the coincident influence of light, oxygen (air), and water. By this photooxidation, saturated as well as unsaturated aliphatic and aromatic compounds, monomeric, polymeric and high polymeric compounds were degraded and the carbon atoms of these compounds as well as carbon itself in form of amorphous carbon or diamond, were converted to carbon dioxide, if dispersed in water or together with water as very small solid, liquid, or gaseous particles. As such conditions occur extensively in nature, it was concluded that this degradation of organic substances is of great importance in nature, in addition to microbial and enzymatic degradation. This photooxidation further represents a possibility for pollution control to prevent damage to environment by degrading organic substances which are either slightly soluble or practically insoluble in water, as well as compounds which will not be destroyed by microbial and enzymatic degradation.", "PMID": 970984}
{"id": "pubmed23n0032_8282", "title": "Effects of DDT in Fundulus: studies on toxicity, fate, and reproduction.", "content": "The toxicity, absorption, distribution, metabolism, and effects on reproduction of DDT was studied using the killifish (Fundulus heteroclitus), a species of economic importance because of its widespread abundance and its presence toward the lower end of the food chain. 14C-DDT was administered by exposure from the ambient water. There was a rapid removal of the radioactive pesticide from the water accompanied by uptake of radioactivity primarily by carcass (primarily muscle tissue) and eggs of the fish. Most (greater than 92%) of the radioactivity in the carcass was shown by TLC methods to be the parent pesticide. One day after a single 24-hr exposure to 14C-DDT, approximately 70% of the administered radioactivity was found in the carcass and the levels of the tissue decayed with a t 1/2 of three days. One day after a single 24-hr exposure to 0.1 ppm of 14C-DDT, the organs that contained the highest concentration of the pesticide (ca. 5 ppm) were intestine and liver. When the pesticide was administered by two 24-hr exposures from water, the intestine, liver and ovaries contained the major concentration of radioactivity (7 to 14 ppm). Untreated Fundulus contained less than 0.2 ppm of total DDT-like compounds. A variety of doses and schedules were tested in an effort to maximize the absorption of DDT, while minimizing the mortality to the fish. An intermittent schedule of 24 hr in 0.1 ppm DDT followed by 24 hr in DDT-free sea water, repeated two times, was found to be optimal. At the levels examined, DDT delayed the rate of normal development of fertilized eggs from Fundulus, but did not appear to cause any observable alterations in the hatched fry. Fertilization of Fundulus eggs was significantly diminished when insemination was carried out in DDT-containing sea water.", "contents": "Effects of DDT in Fundulus: studies on toxicity, fate, and reproduction. The toxicity, absorption, distribution, metabolism, and effects on reproduction of DDT was studied using the killifish (Fundulus heteroclitus), a species of economic importance because of its widespread abundance and its presence toward the lower end of the food chain. 14C-DDT was administered by exposure from the ambient water. There was a rapid removal of the radioactive pesticide from the water accompanied by uptake of radioactivity primarily by carcass (primarily muscle tissue) and eggs of the fish. Most (greater than 92%) of the radioactivity in the carcass was shown by TLC methods to be the parent pesticide. One day after a single 24-hr exposure to 14C-DDT, approximately 70% of the administered radioactivity was found in the carcass and the levels of the tissue decayed with a t 1/2 of three days. One day after a single 24-hr exposure to 0.1 ppm of 14C-DDT, the organs that contained the highest concentration of the pesticide (ca. 5 ppm) were intestine and liver. When the pesticide was administered by two 24-hr exposures from water, the intestine, liver and ovaries contained the major concentration of radioactivity (7 to 14 ppm). Untreated Fundulus contained less than 0.2 ppm of total DDT-like compounds. A variety of doses and schedules were tested in an effort to maximize the absorption of DDT, while minimizing the mortality to the fish. An intermittent schedule of 24 hr in 0.1 ppm DDT followed by 24 hr in DDT-free sea water, repeated two times, was found to be optimal. At the levels examined, DDT delayed the rate of normal development of fertilized eggs from Fundulus, but did not appear to cause any observable alterations in the hatched fry. Fertilization of Fundulus eggs was significantly diminished when insemination was carried out in DDT-containing sea water.", "PMID": 970985}
{"id": "pubmed23n0032_8283", "title": "Uptake and loss of inorganic mercury in the eastern oyster (Crassostrea virginica).", "content": "Eastern oysters (Crassostrea virginica) were found to accumulate significant levels of mercury on exposure to relatively low concentrations of mercuric chloride. Accumulation occurred in two distinct phases which may be described as a two compartment system in which accumulation is logarithmic in the first phase and linear in the second. Mercury, during the initial phase, showed a minimum outward concentration flux of 0.025 hr-1 indicating reversibility of accumulation prior to the change in phase. However, mercury was not excreted at the end of the second phase following exposure to 10, 40, 80, and 100 mug/1 for 256 hr.", "contents": "Uptake and loss of inorganic mercury in the eastern oyster (Crassostrea virginica). Eastern oysters (Crassostrea virginica) were found to accumulate significant levels of mercury on exposure to relatively low concentrations of mercuric chloride. Accumulation occurred in two distinct phases which may be described as a two compartment system in which accumulation is logarithmic in the first phase and linear in the second. Mercury, during the initial phase, showed a minimum outward concentration flux of 0.025 hr-1 indicating reversibility of accumulation prior to the change in phase. However, mercury was not excreted at the end of the second phase following exposure to 10, 40, 80, and 100 mug/1 for 256 hr.", "PMID": 970986}
{"id": "pubmed23n0032_8284", "title": "Effects of weathering and flue-curing on monocrotophos residues on tobacco.", "content": "Disappearance of monocrotophos from flue-cured tobacco was studied at three locations (Kinston, Clayton, and Reidsville, North Carolina) in 1973. Average residues on green tobacco leaves collected immediately after application were 41 and 84 ppm, respectively, for application rates of 0.56 and 1.12 kg/ha. Levels declined to about 18 and 40 ppm for the two rates three days after application, to about 9 and 23 ppm at five days, and to about three and eight ppm at nine days. Conventional curing reduced residues on flue-cured tobacco by at least 99%.", "contents": "Effects of weathering and flue-curing on monocrotophos residues on tobacco. Disappearance of monocrotophos from flue-cured tobacco was studied at three locations (Kinston, Clayton, and Reidsville, North Carolina) in 1973. Average residues on green tobacco leaves collected immediately after application were 41 and 84 ppm, respectively, for application rates of 0.56 and 1.12 kg/ha. Levels declined to about 18 and 40 ppm for the two rates three days after application, to about 9 and 23 ppm at five days, and to about three and eight ppm at nine days. Conventional curing reduced residues on flue-cured tobacco by at least 99%.", "PMID": 970987}
{"id": "pubmed23n0032_8285", "title": "Factors relating to circulating immune complexes in rheumatoid arthirits.", "content": "Evidence has been presented suggesting that circulating immune complexes occur in over half of the sera of patients with rheumatoid arthritis. These IgG-containing complexes were small, eluting between IgG and IgM on gel filtration on Sepharose 6B and were not seen in the sera of healthy control subjects. These complexes were detected in the sera of both seronegative and seropositive patients and their quantity did not correlate with IgM rheumatoid factor titre. The quantity of complexed IgG was estimated from a ratio derived from the IgG profile obtained by gel filtration of the serum. This quantity correlated significantly with the degree of inhibition by the rheumatoid sera of cytolysis in vitro of IgG sensitized target cells by K cells from human peripheral blood. A significant inverse correlation was observed between the quantity of serum complexes and the chemotactic index of the circulating polymorphonuclear leucocytes obtained from the same rheumatoid patient. It is suggested that ingestion of these complexes may be implicated in the reduction in chemotaxis observed in patients with rheumatoid arthritis. There was no correlation between the quantity of the complexes in the sera and the clinica, haematological, and biochemical measurements.", "contents": "Factors relating to circulating immune complexes in rheumatoid arthirits. Evidence has been presented suggesting that circulating immune complexes occur in over half of the sera of patients with rheumatoid arthritis. These IgG-containing complexes were small, eluting between IgG and IgM on gel filtration on Sepharose 6B and were not seen in the sera of healthy control subjects. These complexes were detected in the sera of both seronegative and seropositive patients and their quantity did not correlate with IgM rheumatoid factor titre. The quantity of complexed IgG was estimated from a ratio derived from the IgG profile obtained by gel filtration of the serum. This quantity correlated significantly with the degree of inhibition by the rheumatoid sera of cytolysis in vitro of IgG sensitized target cells by K cells from human peripheral blood. A significant inverse correlation was observed between the quantity of serum complexes and the chemotactic index of the circulating polymorphonuclear leucocytes obtained from the same rheumatoid patient. It is suggested that ingestion of these complexes may be implicated in the reduction in chemotaxis observed in patients with rheumatoid arthritis. There was no correlation between the quantity of the complexes in the sera and the clinica, haematological, and biochemical measurements.", "PMID": 970988}
{"id": "pubmed23n0032_8286", "title": "Immunoglobulin and complement deposition in skin of rheumatoid arthritis and systemic lupus erythematosus patients.", "content": "Rheumatoid arthritis (RA) was differentiated from systemic lupus erythematosus (SLE) by direct immunofluorescent techniques on skin specimens, using monospecific antisera for IgG, IgM, C3, C1q, properdin, and fibrin. Of 30 patients with RA studied, 20 had dermal vessel deposits of immunoglobulins and complement components in unaffected skin without the characteristic dermal-epidermal junctional fluorescence of SLE. Of 24 SLE patients studied, 24 had granular deposits of immunoglobulins and complement components in unaffected skin at the dermal-epidermal junction.", "contents": "Immunoglobulin and complement deposition in skin of rheumatoid arthritis and systemic lupus erythematosus patients. Rheumatoid arthritis (RA) was differentiated from systemic lupus erythematosus (SLE) by direct immunofluorescent techniques on skin specimens, using monospecific antisera for IgG, IgM, C3, C1q, properdin, and fibrin. Of 30 patients with RA studied, 20 had dermal vessel deposits of immunoglobulins and complement components in unaffected skin without the characteristic dermal-epidermal junctional fluorescence of SLE. Of 24 SLE patients studied, 24 had granular deposits of immunoglobulins and complement components in unaffected skin at the dermal-epidermal junction.", "PMID": 970989}
{"id": "pubmed23n0032_8287", "title": "Indomethacin serum concentrations in man. Effects of dosage, food, and antacid.", "content": "In the use of anti-inflammatory compounds, sustained serum levels are thought to be related to drug efficacy. This study shows that frequent clinical administration of indomethacin can result in sustained serum levels of the drug and that food and antacid may have important modifying effects on serum indomethacin concentrations. After oral ingestion by fasting subjects, indomethacin rapidly appeared in the serum, usually reaching peak concentrations in 30 to 90 minutes. Food delayed and decreased the mean peak level; antacid delayed the peak and slightly enhanced subsequent concentrations. With multiple dose schedules plateau levels were reached after 24 hours. When a total daily dose of 150 mg was given as 25 mg every 4 hours peak concentrations were the same but fluctuations were smaller and average concentrations were higher than with a dosage of 50 mg every 8 hours.", "contents": "Indomethacin serum concentrations in man. Effects of dosage, food, and antacid. In the use of anti-inflammatory compounds, sustained serum levels are thought to be related to drug efficacy. This study shows that frequent clinical administration of indomethacin can result in sustained serum levels of the drug and that food and antacid may have important modifying effects on serum indomethacin concentrations. After oral ingestion by fasting subjects, indomethacin rapidly appeared in the serum, usually reaching peak concentrations in 30 to 90 minutes. Food delayed and decreased the mean peak level; antacid delayed the peak and slightly enhanced subsequent concentrations. With multiple dose schedules plateau levels were reached after 24 hours. When a total daily dose of 150 mg was given as 25 mg every 4 hours peak concentrations were the same but fluctuations were smaller and average concentrations were higher than with a dosage of 50 mg every 8 hours.", "PMID": 970990}
{"id": "pubmed23n0032_8288", "title": "Effects of enzyme induction on metabolism of prednisolone. Clinical and laboratory study.", "content": "The addition of phenobarbitone in therapeutic dosage to the drug regimen of prednisolone-treated subjects with rheumatoid arthritis produced measurable deterioration in the clinical status of the patients associated with a more rapid clearance of prednisolone from plasma. It is considered that phenobarbitone induced the hepatic metabolism of prednisolone, effectively reducing the steady state plasma level and resulting in clinical relapse. A slight but significant improvement in the adrenocortical response to tetracosactrin (Synacthen) was noted after phenobarbitone therapy.", "contents": "Effects of enzyme induction on metabolism of prednisolone. Clinical and laboratory study. The addition of phenobarbitone in therapeutic dosage to the drug regimen of prednisolone-treated subjects with rheumatoid arthritis produced measurable deterioration in the clinical status of the patients associated with a more rapid clearance of prednisolone from plasma. It is considered that phenobarbitone induced the hepatic metabolism of prednisolone, effectively reducing the steady state plasma level and resulting in clinical relapse. A slight but significant improvement in the adrenocortical response to tetracosactrin (Synacthen) was noted after phenobarbitone therapy.", "PMID": 970991}
{"id": "pubmed23n0032_8289", "title": "Clinical and radiological survey of the incidence of osteoarthrosis among obese patients.", "content": "Twenty-five grossly obese males were investigated for evidence of osteoarthrosis. A roentgenological survey of multiple joints obtained from 22 of these patients showed few significant degenerative changes. 6 patients (20%) had previously incurred traumatic rents in their menisci necessitating meniscectomy. Our results refute previous claims that obesity is a factor in the genesis of osteoarthrosis but do indicate that obese individuals are more predisposed to traumatic injury of the knee.", "contents": "Clinical and radiological survey of the incidence of osteoarthrosis among obese patients. Twenty-five grossly obese males were investigated for evidence of osteoarthrosis. A roentgenological survey of multiple joints obtained from 22 of these patients showed few significant degenerative changes. 6 patients (20%) had previously incurred traumatic rents in their menisci necessitating meniscectomy. Our results refute previous claims that obesity is a factor in the genesis of osteoarthrosis but do indicate that obese individuals are more predisposed to traumatic injury of the knee.", "PMID": 970992}
{"id": "pubmed23n0032_8290", "title": "Eye inflammation in psoriatic arthritis.", "content": "In a study of 112 patients (49 men, 63 women) with psoriatic arthritis, ocular inflammation was noted in 35 (31.2%). Conjunctivitis was the most common lesion, being found in 19.6% (10 males, 12 females). Iritis occurred in 7.1% (5 men, 3 women), episcleritis in 1.8% (1 man, 1 woman), and keratoconjunctivitis sicca in 2.7% (3 women). 20 patients (10 men, 10 women) had radiological sacroiliitis and 11 of these (7 men, 4 women) had ankylosing spondylitis according to the New York criteria. Eye lesions were noted in 7 of the patients with sacroiliitis, 3 having iritis (15%) and 4 (20%) conjunctivitis. 2 patients with spondylitis had iritis (18%) and 1 (9%) conjunctivitis. It is concluded that inflammatory eye lesions are a frequent accompaniment to psoriatic arthritis. This is taken as further evidence for the concept of the seronegative spondyloarthritides.", "contents": "Eye inflammation in psoriatic arthritis. In a study of 112 patients (49 men, 63 women) with psoriatic arthritis, ocular inflammation was noted in 35 (31.2%). Conjunctivitis was the most common lesion, being found in 19.6% (10 males, 12 females). Iritis occurred in 7.1% (5 men, 3 women), episcleritis in 1.8% (1 man, 1 woman), and keratoconjunctivitis sicca in 2.7% (3 women). 20 patients (10 men, 10 women) had radiological sacroiliitis and 11 of these (7 men, 4 women) had ankylosing spondylitis according to the New York criteria. Eye lesions were noted in 7 of the patients with sacroiliitis, 3 having iritis (15%) and 4 (20%) conjunctivitis. 2 patients with spondylitis had iritis (18%) and 1 (9%) conjunctivitis. It is concluded that inflammatory eye lesions are a frequent accompaniment to psoriatic arthritis. This is taken as further evidence for the concept of the seronegative spondyloarthritides.", "PMID": 970993}
{"id": "pubmed23n0032_8291", "title": "Early rheumatoid disease. I. Onset.", "content": "We describe features with onset in 102 patients seen within the first year of rheumatoid disease. The male:female ratio was approximately 3:4, suggesting a near equal sex incidence at onset. The disease started more often in the colder months and was usually insodious, symmetrical, and involved the upper limbs. The patients were followed prospectively and outcome was assessed after a mean of 4.5 years. Older patients fared worse and there was a trend for a poorer prognosis to be indicated by an insidious onset and early progression to symmetrical involvement.", "contents": "Early rheumatoid disease. I. Onset. We describe features with onset in 102 patients seen within the first year of rheumatoid disease. The male:female ratio was approximately 3:4, suggesting a near equal sex incidence at onset. The disease started more often in the colder months and was usually insodious, symmetrical, and involved the upper limbs. The patients were followed prospectively and outcome was assessed after a mean of 4.5 years. Older patients fared worse and there was a trend for a poorer prognosis to be indicated by an insidious onset and early progression to symmetrical involvement.", "PMID": 970994}
{"id": "pubmed23n0032_8292", "title": "Early rheumatoid disease. II. Patterns of joint involvement.", "content": "Data from the first research clinic visit (Fleming and others, 1976) have been subjected to factor analysis to identify early patterns of joint involvement. Nine patterns emerged. Two patterns, if present early, were found to have prognostic significance. An eventually more severe disease was associated with a pattern of large joint involvement (shoulder, elbow, wrist, knee) and a pattern based on metatarsophalangeal joints I and III.", "contents": "Early rheumatoid disease. II. Patterns of joint involvement. Data from the first research clinic visit (Fleming and others, 1976) have been subjected to factor analysis to identify early patterns of joint involvement. Nine patterns emerged. Two patterns, if present early, were found to have prognostic significance. An eventually more severe disease was associated with a pattern of large joint involvement (shoulder, elbow, wrist, knee) and a pattern based on metatarsophalangeal joints I and III.", "PMID": 970995}
{"id": "pubmed23n0032_8293", "title": "Radiological cervical arthritis in populations.", "content": "The prevalence of cervical rheumatoid arthritis and its relationship to rheumatoid serum factors and erosive arthritis in peripheral joints has been studied in radiographs of the cervical spine and of the hands and feet drawn from 12 population samples. The changes were graded in accordance with the Atlas of Standard Radiographs of Arthritis. Rheumatoid arthritis of the cervical spine (grades 2-4) was observed in 4.1% of males and 4.7% of females aged 15 and over. Prevalence was greatest in those born before 1900, 15% of whom were affected. There was a significant association with the sheep cell agglutination test but not with the bentonite flocculation test, though the latter correlated well with erosive arthritis in the joints of the hands and feet. Arthritis of the cervical spine showed a significant correlation with both seropositive and seronegative erosive arthritis in the peripheral joints. A significantly higher prevalence of cervical arthritis than expected was noted in two population samples, one in Germany and the other in West Africa, though in neither was there a high prevalence of peripheral arthritis. The German population had relatively high antistreptolysin titres. A low prevalence of cervical arthritis was noted in populations in Czechoslovakia and in Arizona. 'Congenital' block vertebra had a prevalence of 0.9% in persons born before 1935, but none was observed in those born since. The figures suggest that environmental influences predisposing to cervical arthritis and block vertebra have changed in the last 40 years.", "contents": "Radiological cervical arthritis in populations. The prevalence of cervical rheumatoid arthritis and its relationship to rheumatoid serum factors and erosive arthritis in peripheral joints has been studied in radiographs of the cervical spine and of the hands and feet drawn from 12 population samples. The changes were graded in accordance with the Atlas of Standard Radiographs of Arthritis. Rheumatoid arthritis of the cervical spine (grades 2-4) was observed in 4.1% of males and 4.7% of females aged 15 and over. Prevalence was greatest in those born before 1900, 15% of whom were affected. There was a significant association with the sheep cell agglutination test but not with the bentonite flocculation test, though the latter correlated well with erosive arthritis in the joints of the hands and feet. Arthritis of the cervical spine showed a significant correlation with both seropositive and seronegative erosive arthritis in the peripheral joints. A significantly higher prevalence of cervical arthritis than expected was noted in two population samples, one in Germany and the other in West Africa, though in neither was there a high prevalence of peripheral arthritis. The German population had relatively high antistreptolysin titres. A low prevalence of cervical arthritis was noted in populations in Czechoslovakia and in Arizona. 'Congenital' block vertebra had a prevalence of 0.9% in persons born before 1935, but none was observed in those born since. The figures suggest that environmental influences predisposing to cervical arthritis and block vertebra have changed in the last 40 years.", "PMID": 970996}
{"id": "pubmed23n0032_8294", "title": "Hypouricaemia and increased renal urate clearance associated with hyperparathyroidism.", "content": "A 64-year-old female was found to have hypouricaemia, with serum uric acid ranging from 0.06-0.12 mmol/l (1.1-2.0 mg/100 ml), associated with an increased urate clearance of 48.9 ml/min and hyperparathyroidism. Known causes of increased uric acid clearance were excluded. Pyrazinamide reduced urate clearance dramatically to 2.1 ml/min, suggesting that the tubular defect was either one of increased secretion or a failure of postsecretory reabsorption. No other tubular abnormality was apparent except diminished urine concentrating ability. Hypouricaemia has not been previously reported in association with hyperparathyroidism and a mechanism relating the two disorders could not be readily postulated. The tubular defect shown in this instance resembled that reported in association with Wilson's disease and Hodgkin's disease. This case and earlier reports of isolated tubular defects of uric acid handling enhance our understanding of uric acid excretion.", "contents": "Hypouricaemia and increased renal urate clearance associated with hyperparathyroidism. A 64-year-old female was found to have hypouricaemia, with serum uric acid ranging from 0.06-0.12 mmol/l (1.1-2.0 mg/100 ml), associated with an increased urate clearance of 48.9 ml/min and hyperparathyroidism. Known causes of increased uric acid clearance were excluded. Pyrazinamide reduced urate clearance dramatically to 2.1 ml/min, suggesting that the tubular defect was either one of increased secretion or a failure of postsecretory reabsorption. No other tubular abnormality was apparent except diminished urine concentrating ability. Hypouricaemia has not been previously reported in association with hyperparathyroidism and a mechanism relating the two disorders could not be readily postulated. The tubular defect shown in this instance resembled that reported in association with Wilson's disease and Hodgkin's disease. This case and earlier reports of isolated tubular defects of uric acid handling enhance our understanding of uric acid excretion.", "PMID": 970997}
{"id": "pubmed23n0032_8295", "title": "Raised serum immunoglobulin E in Wegener's granulomatosis.", "content": "Five patients with Wegner's granulomatosis were found to have significantly raised serum immunoglobulin E (IgE) levels. The rise in IgE was not related to the extent of clinical involvement, was not part of a generalized serum immunoglobulin rise, and was not associated with eosinophilia. Raised serum IgE may be a clue to the pathogenesis of this disease.", "contents": "Raised serum immunoglobulin E in Wegener's granulomatosis. Five patients with Wegner's granulomatosis were found to have significantly raised serum immunoglobulin E (IgE) levels. The rise in IgE was not related to the extent of clinical involvement, was not part of a generalized serum immunoglobulin rise, and was not associated with eosinophilia. Raised serum IgE may be a clue to the pathogenesis of this disease.", "PMID": 970998}
{"id": "pubmed23n0032_8296", "title": "Improvement in lacrimal and salivary secretions after alkali therapy in Sj\u00f8gren's syndrome with renal tubular acidosis.", "content": "A patient with Sj\u00f8gren's syndrome developed renal tubular acidosis which led to systemic acidosis and potassium depletion. Treatment with Shohl's solution and potassium supplements was followed by subjective improvement in tear flow, salivary flow, and by disappearance of bronchitic symptoms. Detailed objective assessments were then made during the next year, twice on treatment and twice without. These confirmed the subjective impression of improvement.", "contents": "Improvement in lacrimal and salivary secretions after alkali therapy in Sj\u00f8gren's syndrome with renal tubular acidosis. A patient with Sj\u00f8gren's syndrome developed renal tubular acidosis which led to systemic acidosis and potassium depletion. Treatment with Shohl's solution and potassium supplements was followed by subjective improvement in tear flow, salivary flow, and by disappearance of bronchitic symptoms. Detailed objective assessments were then made during the next year, twice on treatment and twice without. These confirmed the subjective impression of improvement.", "PMID": 970999}
{"id": "pubmed23n0032_8297", "title": "Host-parasite relations in cotton rat filariasis. I: The quantitative transmission and subsequent development of Litomosoides carinii infections in cotton rats and other laboratory animals.", "content": "This paper introduces the long-term and continuing studies of the authors and their colleagues on several aspects of the host-parasite relations in cotton rat filariasis. Five methods are described for the calculation of the number of infective larvae of Litomosoides carinii transmitted to cotton rats and other laboratory animals by groups of Liponyssus bacoti mites. The relation between the calculated transmission intensities and the number of worms recovered from individual infected animals has been determined by each of the five methods. The accuracy and relevance of each method is discussed and one (method B) has been found to give a better correlation with worm recovery than the others. Some effects of infection with filariasis on the mites are described. The role of heavily infected mites in transmission is questioned and their influence on the calculation of transmission is discussed.", "contents": "Host-parasite relations in cotton rat filariasis. I: The quantitative transmission and subsequent development of Litomosoides carinii infections in cotton rats and other laboratory animals. This paper introduces the long-term and continuing studies of the authors and their colleagues on several aspects of the host-parasite relations in cotton rat filariasis. Five methods are described for the calculation of the number of infective larvae of Litomosoides carinii transmitted to cotton rats and other laboratory animals by groups of Liponyssus bacoti mites. The relation between the calculated transmission intensities and the number of worms recovered from individual infected animals has been determined by each of the five methods. The accuracy and relevance of each method is discussed and one (method B) has been found to give a better correlation with worm recovery than the others. Some effects of infection with filariasis on the mites are described. The role of heavily infected mites in transmission is questioned and their influence on the calculation of transmission is discussed.", "PMID": 971000}
{"id": "pubmed23n0032_8298", "title": "Host-parasite relations in cotton rat filariasis. II: The quantitative transmission of Litomosoides carinii to Delhi and Carworth strains of white rats, including the effect of age.", "content": "Delhi and Carworth strains of white rats were exposed quantitatively to infection with Litomosoides carinii and their susceptibility compared. Liponyssus bacoti (= Ornithonyssus bacoti) mites of known (calculated) intensities of infection were used for transmission. The relation between the calculated exposure (= transmission intensity) and the number of adult worms recovered from each rat was established. Adults of both strains of white rat were much \"poorer\" hosts than cotton rats. All 24 Carworth white rats (100%) and 21/24 of Delhi white rats (87-5%) of differing ages and both sexes became infected. Carworth white rats were generally more susceptible. The susceptibility of newly born Carworth white rats was greatest and was similar to that of cotton rats. Young rats of the Delhi strain were more susceptible than older rats. This strongly suggests \"age resistance\". The degree of resistance may, however, differ in different species and strains of white rats and in individual rats of the same strain.", "contents": "Host-parasite relations in cotton rat filariasis. II: The quantitative transmission of Litomosoides carinii to Delhi and Carworth strains of white rats, including the effect of age. Delhi and Carworth strains of white rats were exposed quantitatively to infection with Litomosoides carinii and their susceptibility compared. Liponyssus bacoti (= Ornithonyssus bacoti) mites of known (calculated) intensities of infection were used for transmission. The relation between the calculated exposure (= transmission intensity) and the number of adult worms recovered from each rat was established. Adults of both strains of white rat were much \"poorer\" hosts than cotton rats. All 24 Carworth white rats (100%) and 21/24 of Delhi white rats (87-5%) of differing ages and both sexes became infected. Carworth white rats were generally more susceptible. The susceptibility of newly born Carworth white rats was greatest and was similar to that of cotton rats. Young rats of the Delhi strain were more susceptible than older rats. This strongly suggests \"age resistance\". The degree of resistance may, however, differ in different species and strains of white rats and in individual rats of the same strain.", "PMID": 971001}
{"id": "pubmed23n0032_8299", "title": "The intervals between the blood-meals of man-biting Simulium damnosum (Diptera: Simuliidae).", "content": "Six marking-recapture experiments were carried out with Simulium damnosum in the Cameroon Republic, five in the rain-forest and one in the Sudan-savanna zone. Adult flies were marked by applying a small spot of oil paint to the mesonotum while they were engorging with blood on the legs of volunteers infected with Onchocerca volvulus microfilariae. Catches of wild flies were carried out for 12-13 days after the first day of marking, and all marked flies recaptured were examined. Some of the flies recaptured on days 0, 1 and 2 were nulliparous and had probably been disturbed by the application of the paint before they had completed their blood-meal. The frequency distribution of recaptured marked parous flies returning for their \"second\" blood-meal rose to a peak early on day 4, but more flies returned earlier,three days after taking a blood-meal, than later on day 5. After day 5, the numbers of recaptured flies were too low to demonstrate any peaks corresponding to \"third\" and later blood-meals. The longest surviving fly was recaptured 10 days after marking. Twenty-six percent of the flies recaptured on days 3 and 4 contained developing O. volvulus larvae three-five days old, which had presumably been ingested as microfilariae during the blood-meal taken on day 0. Infective larvae first appeared in flies returning late on day 6, and the highest percentage of infective flies occurred on day 7. Infective larvae were found in recaptured flies until day 10, the last day on which marked flies were recovered. Of 929 flies marked on the Sudan-savanna experiment, three (0-32%) flies were recaptured. Two returned on day 4 and one on day 6.", "contents": "The intervals between the blood-meals of man-biting Simulium damnosum (Diptera: Simuliidae). Six marking-recapture experiments were carried out with Simulium damnosum in the Cameroon Republic, five in the rain-forest and one in the Sudan-savanna zone. Adult flies were marked by applying a small spot of oil paint to the mesonotum while they were engorging with blood on the legs of volunteers infected with Onchocerca volvulus microfilariae. Catches of wild flies were carried out for 12-13 days after the first day of marking, and all marked flies recaptured were examined. Some of the flies recaptured on days 0, 1 and 2 were nulliparous and had probably been disturbed by the application of the paint before they had completed their blood-meal. The frequency distribution of recaptured marked parous flies returning for their \"second\" blood-meal rose to a peak early on day 4, but more flies returned earlier,three days after taking a blood-meal, than later on day 5. After day 5, the numbers of recaptured flies were too low to demonstrate any peaks corresponding to \"third\" and later blood-meals. The longest surviving fly was recaptured 10 days after marking. Twenty-six percent of the flies recaptured on days 3 and 4 contained developing O. volvulus larvae three-five days old, which had presumably been ingested as microfilariae during the blood-meal taken on day 0. Infective larvae first appeared in flies returning late on day 6, and the highest percentage of infective flies occurred on day 7. Infective larvae were found in recaptured flies until day 10, the last day on which marked flies were recovered. Of 929 flies marked on the Sudan-savanna experiment, three (0-32%) flies were recaptured. Two returned on day 4 and one on day 6.", "PMID": 971002}
{"id": "pubmed23n0032_8300", "title": "Studies on the flight range and dispersal of Simulium damnosum (Diptera: Simuliidae) in the rain-forest of Cameroon.", "content": "Five marking/recapture experiments were carried out on Simulium damnosum in the rain-forest of the United Republic of Cameroon, in order to determine the regression in biting density at increasing distances from the marking point. Flies were captured unfed, marked with fluorescent dust, and then released on the bank of a large river. Recaptures were made over the six-seven days after marking, at seven stations situated from 200 m to 79 km along a large river, from the marking site. Marked flies, which showed fluorescent pigment when examined under an ultraviolet lamp, were recovered at all seven sites. The ratios of the number of recaptures per man-hour at each distant site to the recaptures per man-hour at the marking point were calculated to obtain values for the proportional biting density (Z). The value of Z declined in a logarithmic manner with increasing distance from the marking site, such that it fell to 0-1 at distances of 34-1 km on a large river, 5-5 km on a small river, and 1-5 km on a road leading away from a large river. The peak number of flies recaptured at the farthest sites along a large river (56 km and 79 km) occurred one day later than at the station nearest the marking point. The dispersal of marked flies which had fed on volunteers carrying Onchocerca volvulus microfilariae, was studied along a large river. Of three flies recaptured 24 km upstream from the marking point, one contained 10 infective larvae of O. volvulus.", "contents": "Studies on the flight range and dispersal of Simulium damnosum (Diptera: Simuliidae) in the rain-forest of Cameroon. Five marking/recapture experiments were carried out on Simulium damnosum in the rain-forest of the United Republic of Cameroon, in order to determine the regression in biting density at increasing distances from the marking point. Flies were captured unfed, marked with fluorescent dust, and then released on the bank of a large river. Recaptures were made over the six-seven days after marking, at seven stations situated from 200 m to 79 km along a large river, from the marking site. Marked flies, which showed fluorescent pigment when examined under an ultraviolet lamp, were recovered at all seven sites. The ratios of the number of recaptures per man-hour at each distant site to the recaptures per man-hour at the marking point were calculated to obtain values for the proportional biting density (Z). The value of Z declined in a logarithmic manner with increasing distance from the marking site, such that it fell to 0-1 at distances of 34-1 km on a large river, 5-5 km on a small river, and 1-5 km on a road leading away from a large river. The peak number of flies recaptured at the farthest sites along a large river (56 km and 79 km) occurred one day later than at the station nearest the marking point. The dispersal of marked flies which had fed on volunteers carrying Onchocerca volvulus microfilariae, was studied along a large river. Of three flies recaptured 24 km upstream from the marking point, one contained 10 infective larvae of O. volvulus.", "PMID": 971003}
{"id": "pubmed23n0032_8301", "title": "Effects of larval treatment with the insect development inhibitor PH60:40 on the vectorial capacity of Aedes aegypti (L.) for Brugia pahangi (Buckley and Edeson).", "content": "The effects of PH60:40, an insect development inhibitor, on fourth instar larvae of Aedes aegypti were investigated. Apart from delayed lethal action, possible effects on the vectorial capacity of Brugia pahangi were investigated. Two strains of A. aegypti were used, one DDT-susceptible and a good filarial vector and the other DDT-resistant and a refractory vector. A baseline exposure was found which caused only low mortalities in both strains. The treatments did not have any appreciable effect on the vectorial capacity of the good vector strain, but modified the refractory nature of the poor vector strain. It was considered possible that this could be due to a generalized harmful stress of the treatment rather than to a specific action of the chemical. Larvae were therefore subjected to heat treatments, as an example of stress, and these produced similar mortalities as with PH60:40. They did not. however, alter the vectorial capacity of survivors of either strain, so that the effects described do not seem to be due to simple stress.", "contents": "Effects of larval treatment with the insect development inhibitor PH60:40 on the vectorial capacity of Aedes aegypti (L.) for Brugia pahangi (Buckley and Edeson). The effects of PH60:40, an insect development inhibitor, on fourth instar larvae of Aedes aegypti were investigated. Apart from delayed lethal action, possible effects on the vectorial capacity of Brugia pahangi were investigated. Two strains of A. aegypti were used, one DDT-susceptible and a good filarial vector and the other DDT-resistant and a refractory vector. A baseline exposure was found which caused only low mortalities in both strains. The treatments did not have any appreciable effect on the vectorial capacity of the good vector strain, but modified the refractory nature of the poor vector strain. It was considered possible that this could be due to a generalized harmful stress of the treatment rather than to a specific action of the chemical. Larvae were therefore subjected to heat treatments, as an example of stress, and these produced similar mortalities as with PH60:40. They did not. however, alter the vectorial capacity of survivors of either strain, so that the effects described do not seem to be due to simple stress.", "PMID": 971004}
{"id": "pubmed23n0032_8302", "title": "Electrocardiogram in Hyperkalemia: electrocardiographic pattern of anteroseptal myocardial infarction mimicked by hyperkalemia-induced disturbance of impulse conduction.", "content": "In a patient with renal failure and shortness of breath, Q waves transiently appeared in the right precordial leads of the electrocardiogram (ECG) during episodes of hyperkalemia, without a substantial change in mean electrical axis. With restoration of the plasma potassium level to normal, R waves reappeared in these leads. It is concluded that the transient development of Q waves in the right precordial leads during hyperkalemia resulted from a hyperkalemia-induced conduction disturbance. Hyperkalemia, by affecting conduction in Purkinje fibers of ventricular muscle, or both, disturbed the normal sequence of septal and anterior wall depolarization and resulted in an ECG pattern that mimicked that of anteroseptal myocardial infarction. Clinically, hyperkalemia-induced conduction disturbances of this type must be included in the differential diagnosis of the ECG that suggests an anteroseptal myocardial infarction.", "contents": "Electrocardiogram in Hyperkalemia: electrocardiographic pattern of anteroseptal myocardial infarction mimicked by hyperkalemia-induced disturbance of impulse conduction. In a patient with renal failure and shortness of breath, Q waves transiently appeared in the right precordial leads of the electrocardiogram (ECG) during episodes of hyperkalemia, without a substantial change in mean electrical axis. With restoration of the plasma potassium level to normal, R waves reappeared in these leads. It is concluded that the transient development of Q waves in the right precordial leads during hyperkalemia resulted from a hyperkalemia-induced conduction disturbance. Hyperkalemia, by affecting conduction in Purkinje fibers of ventricular muscle, or both, disturbed the normal sequence of septal and anterior wall depolarization and resulted in an ECG pattern that mimicked that of anteroseptal myocardial infarction. Clinically, hyperkalemia-induced conduction disturbances of this type must be included in the differential diagnosis of the ECG that suggests an anteroseptal myocardial infarction.", "PMID": 971008}
{"id": "pubmed23n0032_8303", "title": "Renal trauma and hypertension: the role of renin.", "content": "Three patients developed hypertension following renal trauma. Trauma produced perinephric hematoma in two and renal artery thrombosis in one. Renal vein plasma renin activity (PRA) from the traumatized kidney was three to eight times greater than renal vein PRA from the untraumatized (contralateral) kidney. Peripheral PRA was elevated in all. A surgical operation lowered peripheral PRA to normal in all, but corrected hypertension in only two of three. Preoperative medical treatment with renin-suppressing pharmacologic agents correctly predicted this response to surgery. Postoperative renal vein PRA in the remaining hypertensive patient demonstrated that surgery successfully alleviated the abnormality in renin secretion. These studies suggest that excessive renin secretion initiate but other unidentified factors may contribute to the hypertension observed after renal trauma.", "contents": "Renal trauma and hypertension: the role of renin. Three patients developed hypertension following renal trauma. Trauma produced perinephric hematoma in two and renal artery thrombosis in one. Renal vein plasma renin activity (PRA) from the traumatized kidney was three to eight times greater than renal vein PRA from the untraumatized (contralateral) kidney. Peripheral PRA was elevated in all. A surgical operation lowered peripheral PRA to normal in all, but corrected hypertension in only two of three. Preoperative medical treatment with renin-suppressing pharmacologic agents correctly predicted this response to surgery. Postoperative renal vein PRA in the remaining hypertensive patient demonstrated that surgery successfully alleviated the abnormality in renin secretion. These studies suggest that excessive renin secretion initiate but other unidentified factors may contribute to the hypertension observed after renal trauma.", "PMID": 971010}
{"id": "pubmed23n0032_8304", "title": "A headache protocol for nurses: effectiveness and efficiency.", "content": "To test the effectiveness and efficiency of a nurse-administered protocol for headache in the primary care setting, we conducted a prospective controlled trial. Two hundred three randomly allocated patients were evaluated by nurses using the protocol who managed independently 55.2% of the patients and received physician consultation on the others. A total of 193 patients were randomly allocated to the physician-control group. Nurse-protocol group patients experienced equivalent symptomatic relief and expressed significantly greater satisfaction with the care they had received than did patients in the physician-control group. A retrospective study of the presenting findings in patients with serious diseases of the CNS provided evidence supporting the safety of the protocol logic. We conclude that nurse-protocol management of headache in this primary care practice was effective and efficient.", "contents": "A headache protocol for nurses: effectiveness and efficiency. To test the effectiveness and efficiency of a nurse-administered protocol for headache in the primary care setting, we conducted a prospective controlled trial. Two hundred three randomly allocated patients were evaluated by nurses using the protocol who managed independently 55.2% of the patients and received physician consultation on the others. A total of 193 patients were randomly allocated to the physician-control group. Nurse-protocol group patients experienced equivalent symptomatic relief and expressed significantly greater satisfaction with the care they had received than did patients in the physician-control group. A retrospective study of the presenting findings in patients with serious diseases of the CNS provided evidence supporting the safety of the protocol logic. We conclude that nurse-protocol management of headache in this primary care practice was effective and efficient.", "PMID": 971011}
{"id": "pubmed23n0032_8305", "title": "Staphylococcal sepsis in patients on chronic hemodialysis regimens: intravenous treatment with vancomycin given once weekly.", "content": "Five patients on chronic hemodialysis regimens were hospitalized with six episodes of Staphylococcus aureus septicemia. Three of the patents acquired the infection during home dialysis, and it was related to the hemodialysis circulatory-access site in four. Initial therapy was methicillin sodium. Vanomycin hydrochloride was given in a 1-gm, single, weekly dose as the sole antibiotic after a short interval (mean, three days). Five of the six episodes were treated successfully. Staphylococcus epidermidis grew in blood cultures obtained during one of the treatments. The advantages of this regimen included a more prompt ambulation and the discontuation of constant infusions in patients with no peripheral veins available.", "contents": "Staphylococcal sepsis in patients on chronic hemodialysis regimens: intravenous treatment with vancomycin given once weekly. Five patients on chronic hemodialysis regimens were hospitalized with six episodes of Staphylococcus aureus septicemia. Three of the patents acquired the infection during home dialysis, and it was related to the hemodialysis circulatory-access site in four. Initial therapy was methicillin sodium. Vanomycin hydrochloride was given in a 1-gm, single, weekly dose as the sole antibiotic after a short interval (mean, three days). Five of the six episodes were treated successfully. Staphylococcus epidermidis grew in blood cultures obtained during one of the treatments. The advantages of this regimen included a more prompt ambulation and the discontuation of constant infusions in patients with no peripheral veins available.", "PMID": 971012}
{"id": "pubmed23n0032_8306", "title": "Exchange transfusion in hepatorenal syndrome with liver disease.", "content": "Ten patients with severe liver disease and the hepatorenal syndrome underwent exchange transfusions with 9 to 16 units of fresh heparinized blood to improve renal function by either removing a vasoconstrictive substance or adding a vasodilatory substance. One patient recovered from renal failure within ten days without showing natriuresis. The renal function of one patient improved somewhat, but he died 35 days after the transfusion. The other eight aptients died from 1 to 35 days after exchange transfusion, without any appreciable improvement in renal function. In six patients, renin substrate levels increased after the transfusion, but renal function remained unchanged. The results of this study failed to support a humoral concept of pathogenesis of the hepatorenal syndrome.", "contents": "Exchange transfusion in hepatorenal syndrome with liver disease. Ten patients with severe liver disease and the hepatorenal syndrome underwent exchange transfusions with 9 to 16 units of fresh heparinized blood to improve renal function by either removing a vasoconstrictive substance or adding a vasodilatory substance. One patient recovered from renal failure within ten days without showing natriuresis. The renal function of one patient improved somewhat, but he died 35 days after the transfusion. The other eight aptients died from 1 to 35 days after exchange transfusion, without any appreciable improvement in renal function. In six patients, renin substrate levels increased after the transfusion, but renal function remained unchanged. The results of this study failed to support a humoral concept of pathogenesis of the hepatorenal syndrome.", "PMID": 971013}
{"id": "pubmed23n0032_8307", "title": "Idiopathic pulmonary hemosiderosis: ultrastructural studies and responses to azathioprine.", "content": "Two boys are presented who fulfilled criteria for a diagnosis of idiopathic pulmonary hemosiderosis. A lung biopsy specimen from the first patient showed alveolar-capillary basement membrane abnormalities, together with abnormalities of capillary endothelial cells and hemosiderin-laden macrophages. A lung biopsy specimen from the second patient showed mainly capillary endothelial abnnormalities and interestitial fibrosis. Both patients had a noticeable improvement in symptoms and relative stabilization of their roentgenographic and pulmonary function abnormalities following azathioprine therapy.", "contents": "Idiopathic pulmonary hemosiderosis: ultrastructural studies and responses to azathioprine. Two boys are presented who fulfilled criteria for a diagnosis of idiopathic pulmonary hemosiderosis. A lung biopsy specimen from the first patient showed alveolar-capillary basement membrane abnormalities, together with abnormalities of capillary endothelial cells and hemosiderin-laden macrophages. A lung biopsy specimen from the second patient showed mainly capillary endothelial abnnormalities and interestitial fibrosis. Both patients had a noticeable improvement in symptoms and relative stabilization of their roentgenographic and pulmonary function abnormalities following azathioprine therapy.", "PMID": 971014}
{"id": "pubmed23n0032_8308", "title": "Tuberculosis of the skull.", "content": "Because tuberculosis of the skull is relatively unusual occurrence, it may not be immediately recognized. It responds readily to chemotherapy and should be suspected in disseminated tuberculosis or in any draining lesion of the skull that is sterile or fails to respond to conventional antibiotic therapy.", "contents": "Tuberculosis of the skull. Because tuberculosis of the skull is relatively unusual occurrence, it may not be immediately recognized. It responds readily to chemotherapy and should be suspected in disseminated tuberculosis or in any draining lesion of the skull that is sterile or fails to respond to conventional antibiotic therapy.", "PMID": 971015}
{"id": "pubmed23n0032_8309", "title": "Prolactin-secreting pituitary tumor in early adolescence: hormonal and electron microscopical studies.", "content": "A 13-year-old boy was evaluated for an enlarged sella. There were no consistent visual field defects, and carotid arteriograms and pneumoencephalograms showed no extrasellar abnormality. The endocrine workup demonstrated a subnormal growth hormone reserve. The only other, but most striking abnormality, was an excessively high serum prolactin level, which was partially suppressed with levodopa. A chromophobe pituitary tumor was removed that had histological features suggestive of malignant neoplasm. Characteristics of a prolactin-secreting tumor were confirmed by electron microscopy. The serum prolactin levels decreased substantially  after surgery and further after a course of pituitary irradiation; however, they continue to be elevated above normal. Prolactin determinations play in integral part in the evaluation for a possible pituitary tumor-not only for diagnostic purposes, but also to provide a parameter of future tumor activity in an established case. Electron microscopical studies are helpful in identifying the tumor as source of the excessive prolactin secretion.", "contents": "Prolactin-secreting pituitary tumor in early adolescence: hormonal and electron microscopical studies. A 13-year-old boy was evaluated for an enlarged sella. There were no consistent visual field defects, and carotid arteriograms and pneumoencephalograms showed no extrasellar abnormality. The endocrine workup demonstrated a subnormal growth hormone reserve. The only other, but most striking abnormality, was an excessively high serum prolactin level, which was partially suppressed with levodopa. A chromophobe pituitary tumor was removed that had histological features suggestive of malignant neoplasm. Characteristics of a prolactin-secreting tumor were confirmed by electron microscopy. The serum prolactin levels decreased substantially  after surgery and further after a course of pituitary irradiation; however, they continue to be elevated above normal. Prolactin determinations play in integral part in the evaluation for a possible pituitary tumor-not only for diagnostic purposes, but also to provide a parameter of future tumor activity in an established case. Electron microscopical studies are helpful in identifying the tumor as source of the excessive prolactin secretion.", "PMID": 971016}
{"id": "pubmed23n0032_8310", "title": "Bone-marrow imaging in pure red blood cell aplasia.", "content": "Bone-marrow scintigraphy with indium chloride in 111 was performed on a patient with pure red blood cell aplasia before and after successful treatment with immunosuppressive drugs. The return of erythroid precursors to the bone marrow was accompanied by a substantial increase in the marrow uptake of 111In. The distribution of 111In in the posttreatment scan was indistinguishable from that of 52Fe. These results indicate that indium chloride in 111 is a useful agent for the delineation of erythroid cellularity within bone marrow.", "contents": "Bone-marrow imaging in pure red blood cell aplasia. Bone-marrow scintigraphy with indium chloride in 111 was performed on a patient with pure red blood cell aplasia before and after successful treatment with immunosuppressive drugs. The return of erythroid precursors to the bone marrow was accompanied by a substantial increase in the marrow uptake of 111In. The distribution of 111In in the posttreatment scan was indistinguishable from that of 52Fe. These results indicate that indium chloride in 111 is a useful agent for the delineation of erythroid cellularity within bone marrow.", "PMID": 971017}
{"id": "pubmed23n0032_8311", "title": "Hyporeninemic hypoaldosteronism after chronic sodium bicarbonate abuse.", "content": "We describe a 52-year-old man who, following the discontinuation of massive intake of sodium bicarbonate, in the absence of renal insufficiency, developed progressive hyperkalemia and hyponatremia in association with hyporeninemic hypoaldosteronism. The mechanism of this syndrome is not known, but may involve suppression of the renin-angiotensin-aldosterone axis by chronic volume expansion.", "contents": "Hyporeninemic hypoaldosteronism after chronic sodium bicarbonate abuse. We describe a 52-year-old man who, following the discontinuation of massive intake of sodium bicarbonate, in the absence of renal insufficiency, developed progressive hyperkalemia and hyponatremia in association with hyporeninemic hypoaldosteronism. The mechanism of this syndrome is not known, but may involve suppression of the renin-angiotensin-aldosterone axis by chronic volume expansion.", "PMID": 971018}
{"id": "pubmed23n0032_8312", "title": "Surgical correction of cardiogenic shock.", "content": "Cardiogenic shock as a result of myocardial infarction demands an aggressive diagnostic and therapeutic approach to improve survival. First, the patient likely to develop shock should be identified early in his course so that interventions to reduce or contain infarct progression and thus prevent shock can be instituted. If the patient's condition deteriorates, prompt precise diagnosis of the cause of the hemodyanamic impairment must be made at the bedside with Swan-Ganz right heart catheterization and echocardiography, and, if potentially surgically correctable disease is present, left heart catheterization with coronary angiography in the catheterization laboratory should be done. Surgical repair of anatomic disruptions of the heart ventricular septal defect, or mitral valve rupture is frequently life-saving. At present, operative intervention for shock secondary to massive myocardial infarction alone remains a challenge.", "contents": "Surgical correction of cardiogenic shock. Cardiogenic shock as a result of myocardial infarction demands an aggressive diagnostic and therapeutic approach to improve survival. First, the patient likely to develop shock should be identified early in his course so that interventions to reduce or contain infarct progression and thus prevent shock can be instituted. If the patient's condition deteriorates, prompt precise diagnosis of the cause of the hemodyanamic impairment must be made at the bedside with Swan-Ganz right heart catheterization and echocardiography, and, if potentially surgically correctable disease is present, left heart catheterization with coronary angiography in the catheterization laboratory should be done. Surgical repair of anatomic disruptions of the heart ventricular septal defect, or mitral valve rupture is frequently life-saving. At present, operative intervention for shock secondary to massive myocardial infarction alone remains a challenge.", "PMID": 971019}
{"id": "pubmed23n0032_8313", "title": "A study of \"atypical schizophrenia\". Comparison with schizophrenia and affective disorder by sex, age of admission, precipitant, outcome, and family history.", "content": "Eighty-five cases of atypical schizophrenia were compared with 200 of schizophrenia, 100 of bipolar (mania), and 225 of unipolar (depression) affective disorder. Comparisons were made on the basis of sex, age at admission, precipitating factors, outcome, and a family history of schizophrenia or of affective disorder. The atypical schizophrenia differed remarkably from the schizophrenia and most closely resembled the bipolar affective disorder when allowance was made for a younger age at onset and a higher frequency of precipitants. An analysis of symptoms verified the predominance of schizophrenic features in the atypical schizophrenia, but also showed a high percentage (80%) of patients who had one or more manic symptoms at index admission. It is concluded that great care should be taken in diagnosing schizophrenia in a patient who also has manic symptoms.", "contents": "A study of \"atypical schizophrenia\". Comparison with schizophrenia and affective disorder by sex, age of admission, precipitant, outcome, and family history. Eighty-five cases of atypical schizophrenia were compared with 200 of schizophrenia, 100 of bipolar (mania), and 225 of unipolar (depression) affective disorder. Comparisons were made on the basis of sex, age at admission, precipitating factors, outcome, and a family history of schizophrenia or of affective disorder. The atypical schizophrenia differed remarkably from the schizophrenia and most closely resembled the bipolar affective disorder when allowance was made for a younger age at onset and a higher frequency of precipitants. An analysis of symptoms verified the predominance of schizophrenic features in the atypical schizophrenia, but also showed a high percentage (80%) of patients who had one or more manic symptoms at index admission. It is concluded that great care should be taken in diagnosing schizophrenia in a patient who also has manic symptoms.", "PMID": 971026}
{"id": "pubmed23n0032_8314", "title": "Etiological factors in manic-depressive psychoses.", "content": "Fifty consecutive patients with bipolar affective psychosis are reviewed, and the genetic and electroencephalographic findings set against extant hypotheses about inheritance of the disorder and the etiological homogeneity of the syndrome. Inheritance in this sample appears usually not to be X-linked. The syndrome seems etiologically heterogeneous in that some patients have an early onset and prominent genetic determinants, while in others without apparent hereditary predisposition, the illness start later and show electroencephalographic evidence of cerebral dysfunction.", "contents": "Etiological factors in manic-depressive psychoses. Fifty consecutive patients with bipolar affective psychosis are reviewed, and the genetic and electroencephalographic findings set against extant hypotheses about inheritance of the disorder and the etiological homogeneity of the syndrome. Inheritance in this sample appears usually not to be X-linked. The syndrome seems etiologically heterogeneous in that some patients have an early onset and prominent genetic determinants, while in others without apparent hereditary predisposition, the illness start later and show electroencephalographic evidence of cerebral dysfunction.", "PMID": 971027}
{"id": "pubmed23n0032_8315", "title": "5-HIAA in the cerebrospinal fluid. A biochemical suicide predictor?", "content": "The incidence of suicidal acts was studied in 68 depressed patients and related to the level of 5-hydroxyindoleacetic acid (5-HIAA) in the cerebrospinal fluid. The distribution of 5-HIAA levels was bimodal. Patients in the low 5-HIAA mode (below 15 ng/ml) attempted suicide significantly more often than those in the high mode, and they used more violent means. Two of the 20 patients in the low mode, and none of the 48 patients in the high mode died from suicide.", "contents": "5-HIAA in the cerebrospinal fluid. A biochemical suicide predictor? The incidence of suicidal acts was studied in 68 depressed patients and related to the level of 5-hydroxyindoleacetic acid (5-HIAA) in the cerebrospinal fluid. The distribution of 5-HIAA levels was bimodal. Patients in the low 5-HIAA mode (below 15 ng/ml) attempted suicide significantly more often than those in the high mode, and they used more violent means. Two of the 20 patients in the low mode, and none of the 48 patients in the high mode died from suicide.", "PMID": 971028}
{"id": "pubmed23n0032_8316", "title": "Glucose-6-phosphate dehydrogenase deficiency with psychosis.", "content": "Several authors have attempted to establish a correlation between glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and chronic schizophrenia, and the results were contradictory. We propose that the correlation between G-6-PD deficiency and schizophrenia is to be found in the form of an acute delirium.", "contents": "Glucose-6-phosphate dehydrogenase deficiency with psychosis. Several authors have attempted to establish a correlation between glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and chronic schizophrenia, and the results were contradictory. We propose that the correlation between G-6-PD deficiency and schizophrenia is to be found in the form of an acute delirium.", "PMID": 971029}
{"id": "pubmed23n0032_8317", "title": "Effects of isometric exercise on serum creatine phosphokinase activity.", "content": "The effect of isometric exercise on serum creatinine phosphokinase (CPK) activity in 14 psychotic patients in remission and ten normal controls was studied. The increases in serum CPK activity at 18 and 42 hours after exercise were no significantly different in patients and controls. The postexercise serum CPK activities in the patients were significantly less than the peak serum CPK levels when they were psychotic. There were no significant correlations between postexercise serum CPK activity and preexercise or peak serum CPK activity in the patient group. It is unlikely that increased isometric muscle tension is a major causative factor in the increased serum CPK levels frequently found in psychotic patients.", "contents": "Effects of isometric exercise on serum creatine phosphokinase activity. The effect of isometric exercise on serum creatinine phosphokinase (CPK) activity in 14 psychotic patients in remission and ten normal controls was studied. The increases in serum CPK activity at 18 and 42 hours after exercise were no significantly different in patients and controls. The postexercise serum CPK activities in the patients were significantly less than the peak serum CPK levels when they were psychotic. There were no significant correlations between postexercise serum CPK activity and preexercise or peak serum CPK activity in the patient group. It is unlikely that increased isometric muscle tension is a major causative factor in the increased serum CPK levels frequently found in psychotic patients.", "PMID": 971030}
{"id": "pubmed23n0032_8318", "title": "Comparative effects of methylphenidate and thioridazine in hyperkinetic children. I. Clinical results.", "content": "The effects of three pharmacological treatments, methylphenidate hydrochloride, thioridazine hydrochloride, a methylphenidate/thioridazine combination, and placebo were studied in outpatient hyperkinetic children rated hyperactive both in school and at home or clinic. Active treatment lasted 12 weeks; placebo lasted four weeks. Significant clinical improvement was obtained in a variety of settings--all treatments were superior to placebo on ratings filled out by parents, teachers, and clinic staff. Though initially the combination of methylphenidate and thioridazine tended to produce greater clinical improvement, it was not superior to methylphenidate alone after 12 weeks of treatment. Methylphenidate alone and the methylphenidate/thioridazine combination were more effective than thioridazine alone. The salient side effects with methylphenidate treatment were decrease in appetite, difficulty in falling asleep, and increased mood sensitivity. In contrast, thioridazine administration was associated with appetite increase and enuresis.", "contents": "Comparative effects of methylphenidate and thioridazine in hyperkinetic children. I. Clinical results. The effects of three pharmacological treatments, methylphenidate hydrochloride, thioridazine hydrochloride, a methylphenidate/thioridazine combination, and placebo were studied in outpatient hyperkinetic children rated hyperactive both in school and at home or clinic. Active treatment lasted 12 weeks; placebo lasted four weeks. Significant clinical improvement was obtained in a variety of settings--all treatments were superior to placebo on ratings filled out by parents, teachers, and clinic staff. Though initially the combination of methylphenidate and thioridazine tended to produce greater clinical improvement, it was not superior to methylphenidate alone after 12 weeks of treatment. Methylphenidate alone and the methylphenidate/thioridazine combination were more effective than thioridazine alone. The salient side effects with methylphenidate treatment were decrease in appetite, difficulty in falling asleep, and increased mood sensitivity. In contrast, thioridazine administration was associated with appetite increase and enuresis.", "PMID": 971031}
{"id": "pubmed23n0032_8319", "title": "Hospital psychiatry in transition. From the therapeutic community toward a rational eclecticism.", "content": "Concepts derived from the paradigm of the therapeutic community have provided the main organizing principles for general inpatient psychiatry in the past 20 years. Increasingly, however, scientific studies have thrown doubt on the efficacy of current hospital practices. Changes in the broader social milieu have occurred as well since the therapeutic community was originally conceptualized, which raise questions about the \"fit\" between milieu therapy principles and current psychiatric needs. No current therapeutic paradigm is sufficient to encompass current inpatient psychiatric practice. There is a need for flexibility in utilizing various organizing principles for various aspects of the problems at hand. The paradigm of \"problem-solving,\" derived from the research findings comparing group vs individual problem-solving, is an example of one way of examining and organizing data that could be applied to certain aspects of hospital care.", "contents": "Hospital psychiatry in transition. From the therapeutic community toward a rational eclecticism. Concepts derived from the paradigm of the therapeutic community have provided the main organizing principles for general inpatient psychiatry in the past 20 years. Increasingly, however, scientific studies have thrown doubt on the efficacy of current hospital practices. Changes in the broader social milieu have occurred as well since the therapeutic community was originally conceptualized, which raise questions about the \"fit\" between milieu therapy principles and current psychiatric needs. No current therapeutic paradigm is sufficient to encompass current inpatient psychiatric practice. There is a need for flexibility in utilizing various organizing principles for various aspects of the problems at hand. The paradigm of \"problem-solving,\" derived from the research findings comparing group vs individual problem-solving, is an example of one way of examining and organizing data that could be applied to certain aspects of hospital care.", "PMID": 971032}
{"id": "pubmed23n0032_8320", "title": "Two occult causes of insomnia and their therapeutic problems.", "content": "Insomnia may be associated with a sleep-induced apnea syndrome in nonobese patients who snore. The \"central\" type of apnea appears to be predominant in this population, in opposition to Pickwickian and nonobese hypersomniacs. An abnormal \"swallowing reflex syndrome,\" also induced by sleep, may be a differential diagnosis. Sleeping pills that are central nervous system depressants should be cautiously prescribed for patients with such syndromes.", "contents": "Two occult causes of insomnia and their therapeutic problems. Insomnia may be associated with a sleep-induced apnea syndrome in nonobese patients who snore. The \"central\" type of apnea appears to be predominant in this population, in opposition to Pickwickian and nonobese hypersomniacs. An abnormal \"swallowing reflex syndrome,\" also induced by sleep, may be a differential diagnosis. Sleeping pills that are central nervous system depressants should be cautiously prescribed for patients with such syndromes.", "PMID": 971033}
{"id": "pubmed23n0032_8321", "title": "Cognitive performance in conversion hysteria.", "content": "In order to test some neurobiologically based assumptions pertaining to attention and memory dysfunction in conversion hysteria, a series of tasks was given to 17 hospitalized patients with hysterical conversion reaction and to a control group of nonpsychotic patients under conditions of nonstress and stress. The results indicated significant differences in performance between hysteria and control subjects. The former group, in comparison to controls, had heightened suggestibility, greater field dependency, and greater impairment of recent memory and vigilance-attention. A discriminant analysis indicated the feasibility of using such tests as objective diagnostic criteria for hysteria.", "contents": "Cognitive performance in conversion hysteria. In order to test some neurobiologically based assumptions pertaining to attention and memory dysfunction in conversion hysteria, a series of tasks was given to 17 hospitalized patients with hysterical conversion reaction and to a control group of nonpsychotic patients under conditions of nonstress and stress. The results indicated significant differences in performance between hysteria and control subjects. The former group, in comparison to controls, had heightened suggestibility, greater field dependency, and greater impairment of recent memory and vigilance-attention. A discriminant analysis indicated the feasibility of using such tests as objective diagnostic criteria for hysteria.", "PMID": 971034}
{"id": "pubmed23n0032_8322", "title": "Hypnotizability and phobic behavior.", "content": "Hypnotizability ratings of 24 phobic patients interested in the therapeutic use of hypnosis were compared with those of an equal number of smokers keen to quit smoking through hypnosis. The mean Stanford Hypnotic Susceptibility Scale score of phobics was 8.08 on a 12-point scale. The mean of smokers was 6.08. The difference was significant beyond the .01 level (two-tailed). Thirty percent of smokers were essentially nonresponsive. No phobics were nonresponsive. Those with multiple phobias scored more highly than those with a single phobia. These findings are in accord with the view that among psychiatric patients whose hypnotizability is assessed in a treatment context, hysterics are most responsive.  The implications both for theory and for a specific treatment strategy are discussed.", "contents": "Hypnotizability and phobic behavior. Hypnotizability ratings of 24 phobic patients interested in the therapeutic use of hypnosis were compared with those of an equal number of smokers keen to quit smoking through hypnosis. The mean Stanford Hypnotic Susceptibility Scale score of phobics was 8.08 on a 12-point scale. The mean of smokers was 6.08. The difference was significant beyond the .01 level (two-tailed). Thirty percent of smokers were essentially nonresponsive. No phobics were nonresponsive. Those with multiple phobias scored more highly than those with a single phobia. These findings are in accord with the view that among psychiatric patients whose hypnotizability is assessed in a treatment context, hysterics are most responsive.  The implications both for theory and for a specific treatment strategy are discussed.", "PMID": 971035}
{"id": "pubmed23n0032_8323", "title": "A thrice-told tale of therapy.", "content": "A case history twice discussed previously in the literature is reexamined. The patient probably suffered a manic-depressive illness, and remission, therefore, is not specifically attributable to psychotherapy. The significance of psychotherapy in the patient's treatment is argued, but for its supportive, not its curative, effects.", "contents": "A thrice-told tale of therapy. A case history twice discussed previously in the literature is reexamined. The patient probably suffered a manic-depressive illness, and remission, therefore, is not specifically attributable to psychotherapy. The significance of psychotherapy in the patient's treatment is argued, but for its supportive, not its curative, effects.", "PMID": 971036}
{"id": "pubmed23n0032_8324", "title": "Regression in the service of residency education.", "content": "Regression as a potentially adaptive psychological response is used as a conceptual model to understand a variety of behaviors seen in beginning psychiatric residents on an inpatient service. The behaviors, discussed and illustrated with brief examples, are (1) competition and identification, (2) sexuality, (3) aggression, (4) depression and despair, (5) dependency, and (6) fusion with patients. Factors on an inpatient service that foster regressive behavior in new residents are discussed, and some of the potential resolutions of the behavoirs are proposed. Regression is seen as an adaptive experience for most residents; with appropriate supervisory intervention, it can foster cognitive development.", "contents": "Regression in the service of residency education. Regression as a potentially adaptive psychological response is used as a conceptual model to understand a variety of behaviors seen in beginning psychiatric residents on an inpatient service. The behaviors, discussed and illustrated with brief examples, are (1) competition and identification, (2) sexuality, (3) aggression, (4) depression and despair, (5) dependency, and (6) fusion with patients. Factors on an inpatient service that foster regressive behavior in new residents are discussed, and some of the potential resolutions of the behavoirs are proposed. Regression is seen as an adaptive experience for most residents; with appropriate supervisory intervention, it can foster cognitive development.", "PMID": 971037}
{"id": "pubmed23n0032_8325", "title": "Psychiatric consultation education--1976.", "content": "The teaching of consultation liaison (CL) psychiatry to psychiatric residents, medical students, and other trainees is reported from 92 medical schools, representing 81% of those surveyed with a one-page questionnaire. Psychiatric residency programs devote approximately 10% of their time to CL training, a slight increase over the percentage ten years ago.  Approximately 35% of medical students in programs from which we received responses have some consultation CL education, although this varies from a few seminars to an eight-week full-time rotation. Other trainees receiving CL experience include nonpsychiatric residents and interns (22 programs), psychologists (18), nurses (12), and social workers (5). Program directors report an approximately 90% \"favorable\" reaction to such training by all trainees. Consultation liaison training for residents still represents a small portion of their entire experience. A surprisingly large percentage of medical students are exposed to CL education, but with tremendous variation in that exposure. Other trainees are not heavily involved in CL programs, indicating a possible future expansion of integrated psychosomatic teaching. The data presented should serve as a comparative baseline for future program design.", "contents": "Psychiatric consultation education--1976. The teaching of consultation liaison (CL) psychiatry to psychiatric residents, medical students, and other trainees is reported from 92 medical schools, representing 81% of those surveyed with a one-page questionnaire. Psychiatric residency programs devote approximately 10% of their time to CL training, a slight increase over the percentage ten years ago.  Approximately 35% of medical students in programs from which we received responses have some consultation CL education, although this varies from a few seminars to an eight-week full-time rotation. Other trainees receiving CL experience include nonpsychiatric residents and interns (22 programs), psychologists (18), nurses (12), and social workers (5). Program directors report an approximately 90% \"favorable\" reaction to such training by all trainees. Consultation liaison training for residents still represents a small portion of their entire experience. A surprisingly large percentage of medical students are exposed to CL education, but with tremendous variation in that exposure. Other trainees are not heavily involved in CL programs, indicating a possible future expansion of integrated psychosomatic teaching. The data presented should serve as a comparative baseline for future program design.", "PMID": 971038}
{"id": "pubmed23n0032_8326", "title": "[Experimental heart tumours in rats (author's transl)].", "content": "In continuation of previous own investigations 68 heart tumours including 46 early stages were found in the heart of 590 BD IX rats systematically examined after treatment with methylnitrosourea (MNU) or ethylnitrosourea (ENU). The tumour incidence was about 40% in animals treated by repeated intravenous or intraperitoneal injections of 20 mg per kg body weight MNU. These experiments are suitable for the study of early stages and the histogenetic origin of heart tumours. At present MNU in addition to triazenes (table 5) belongs to the most powerful carcinogens which affect the heart in rats.", "contents": "[Experimental heart tumours in rats (author's transl)]. In continuation of previous own investigations 68 heart tumours including 46 early stages were found in the heart of 590 BD IX rats systematically examined after treatment with methylnitrosourea (MNU) or ethylnitrosourea (ENU). The tumour incidence was about 40% in animals treated by repeated intravenous or intraperitoneal injections of 20 mg per kg body weight MNU. These experiments are suitable for the study of early stages and the histogenetic origin of heart tumours. At present MNU in addition to triazenes (table 5) belongs to the most powerful carcinogens which affect the heart in rats.", "PMID": 971039}
{"id": "pubmed23n0032_8327", "title": "[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)].", "content": "A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.", "contents": "[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)]. A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.", "PMID": 971040}
{"id": "pubmed23n0032_8328", "title": "[Evaluation of Brdicka's reaction in tumor diagnostic (author's transl)].", "content": "We have investigated the polarographic behaviour of sera from patients of an ORL-department by means of Brdicka reaction. It could be shown, that patients with cancer or acute inflammation had significantly increased values. But the arithmetic mean of this groups is enclosed by the scattering of the normal collective, therefore is no practically use for diagnostic purposes possible.", "contents": "[Evaluation of Brdicka's reaction in tumor diagnostic (author's transl)]. We have investigated the polarographic behaviour of sera from patients of an ORL-department by means of Brdicka reaction. It could be shown, that patients with cancer or acute inflammation had significantly increased values. But the arithmetic mean of this groups is enclosed by the scattering of the normal collective, therefore is no practically use for diagnostic purposes possible.", "PMID": 971041}
{"id": "pubmed23n0032_8329", "title": "[The characterisation of malignant ovarian tumors by impulse cytophotometry (author's transl)].", "content": "31 malignant adnexal tumors were investigated by impulse cytophotometry. From the DNS distribution curves we interpreted the positions and the height of the peaks in order to get informations about ploidy and proliferation pattern. 45.2% were classified into the diploid, 29.0% into the polyploid and 12.9% into the tetraploid group. 12.9% were aneuploid. Comparing our results with chromosome analyses in the literature we found more tetraploid tumors in our material. For our polyploid curves we assume, that many of the cells in the 8c-peak remain in the corresponding G2'-Phase, not undergoing mitosis.", "contents": "[The characterisation of malignant ovarian tumors by impulse cytophotometry (author's transl)]. 31 malignant adnexal tumors were investigated by impulse cytophotometry. From the DNS distribution curves we interpreted the positions and the height of the peaks in order to get informations about ploidy and proliferation pattern. 45.2% were classified into the diploid, 29.0% into the polyploid and 12.9% into the tetraploid group. 12.9% were aneuploid. Comparing our results with chromosome analyses in the literature we found more tetraploid tumors in our material. For our polyploid curves we assume, that many of the cells in the 8c-peak remain in the corresponding G2'-Phase, not undergoing mitosis.", "PMID": 971042}
{"id": "pubmed23n0032_8330", "title": "[Thoughts about the present state of tumour chemotherapy (author's transl)].", "content": "There are some impacts in tumour chemotherapy being worth-while to be discussed. Considering the information received during the Ninth International Congress of Chemotherapy, held in 1975, the author tries to evaluate the present state of clinical tumour chemotherapy from clinical point of view. The modern oncological clinician (oncologist) has to be faced with a lot of new facts, e.g. use of quantitative differences between normal and tumour cells for individualization of therapy, timing of cancer drug administration, new drugs for cancer treatment, mutual inhibition or potentiation of drug's effects, immunotherapy and possible adverse effects of clinically available drugs.", "contents": "[Thoughts about the present state of tumour chemotherapy (author's transl)]. There are some impacts in tumour chemotherapy being worth-while to be discussed. Considering the information received during the Ninth International Congress of Chemotherapy, held in 1975, the author tries to evaluate the present state of clinical tumour chemotherapy from clinical point of view. The modern oncological clinician (oncologist) has to be faced with a lot of new facts, e.g. use of quantitative differences between normal and tumour cells for individualization of therapy, timing of cancer drug administration, new drugs for cancer treatment, mutual inhibition or potentiation of drug's effects, immunotherapy and possible adverse effects of clinically available drugs.", "PMID": 971043}
{"id": "pubmed23n0032_8331", "title": "Inhibition of subcutaneous Ehrlich carcinoma by antihuman-somatotropic hormone serum of the horse as determined by the tumour-tetanus assay of the mouse.", "content": "Utilizing a quantitative tumour tetanus assay of the mouse it could be shown that antisomatotropic hormone serum of the horse exerts protective effects against solid Ehrlich carcinoma of the subcutaneous tissue and concomitantly, against tetanus mortality rates as an indicator of tumour growth. The positive correlation of the inhibitory action of antisomatotropic hormone serum against both transplantable tumour cells and subcutaneous regenerative tissue as a control lends further support to the assumption that a selective mechanism interrelates proliferative mammalian cell populations and multiplying toxinogenic clostridia, thus stimulating a new field of application of antisomatotropic hormone serum.", "contents": "Inhibition of subcutaneous Ehrlich carcinoma by antihuman-somatotropic hormone serum of the horse as determined by the tumour-tetanus assay of the mouse. Utilizing a quantitative tumour tetanus assay of the mouse it could be shown that antisomatotropic hormone serum of the horse exerts protective effects against solid Ehrlich carcinoma of the subcutaneous tissue and concomitantly, against tetanus mortality rates as an indicator of tumour growth. The positive correlation of the inhibitory action of antisomatotropic hormone serum against both transplantable tumour cells and subcutaneous regenerative tissue as a control lends further support to the assumption that a selective mechanism interrelates proliferative mammalian cell populations and multiplying toxinogenic clostridia, thus stimulating a new field of application of antisomatotropic hormone serum.", "PMID": 971044}
{"id": "pubmed23n0032_8332", "title": "Mixed organ culture as a tool for considering cellular tumor-host relationships in tumor patients.", "content": "A method is reported for considering tumour-host relationships, which consists in cultivation of human tumours jointly with autologous lymphocytes in an organ culture system and determining separately tumour and lymphocyte DNA synthesis before and after mixed culture. Some experiments explore variables of the new system. For mixed organ cultures the best conditions are: 4-day incubation, lymphocyte DNA ratio to tumour cell DNA less than 9:1 (2-10(6) lymphocytes/ml) and autologous serum. So far 41 tumours (20 mammary, 17 gastric, and 4 bronchial carcinomas) have been examined. 22 tumours showed satisfactory in vitro maintenance. In 50% of the mixed organ cultures, lymphocyte DNA synthesis proved to be higher than in lymphocyte control cultures. Blast counts in the cell smears are in good accord with results obtained by biochemical determination of DNA synthesis. 7 of the tumours studied have shown a pronounced reduction of DNA synthesis in the presence of lymphocytes. The present results underline the individuality of tumour-host relationships and suggest the necessity of determinations of the immune reactivity in cancer patients. Advantages and disadvantages of the method are discussed.", "contents": "Mixed organ culture as a tool for considering cellular tumor-host relationships in tumor patients. A method is reported for considering tumour-host relationships, which consists in cultivation of human tumours jointly with autologous lymphocytes in an organ culture system and determining separately tumour and lymphocyte DNA synthesis before and after mixed culture. Some experiments explore variables of the new system. For mixed organ cultures the best conditions are: 4-day incubation, lymphocyte DNA ratio to tumour cell DNA less than 9:1 (2-10(6) lymphocytes/ml) and autologous serum. So far 41 tumours (20 mammary, 17 gastric, and 4 bronchial carcinomas) have been examined. 22 tumours showed satisfactory in vitro maintenance. In 50% of the mixed organ cultures, lymphocyte DNA synthesis proved to be higher than in lymphocyte control cultures. Blast counts in the cell smears are in good accord with results obtained by biochemical determination of DNA synthesis. 7 of the tumours studied have shown a pronounced reduction of DNA synthesis in the presence of lymphocytes. The present results underline the individuality of tumour-host relationships and suggest the necessity of determinations of the immune reactivity in cancer patients. Advantages and disadvantages of the method are discussed.", "PMID": 971045}
{"id": "pubmed23n0032_8333", "title": "[Cellelectrophoretic studies of the effect of methylglyoxal-bis-guanylhydrazone on blood cells of tumour patients (author's transl)].", "content": "The effect of methylglyoxal-bis-guanylhydrazone has been studied on the electrophoretic mobility of blood cells of 10 unselected and untreated patients with preferably solid malignant diseases. Prior to the incubation with CH3-G the electrophoretic mobility of lymphocytes and erythrocytes showed increased and of platelets showed decreased rates which extend over a larger range than usual and for which reasons cannot be given till now. The incubation with CH3-G caused a concentration-dependent reduction of the electrophoretic mobility of all three tested types of blood cells. This can be estimated as an impairment of a function of surface membrane and confirms recently obtained results of activity of CH3-G on platelets' surface membrane.", "contents": "[Cellelectrophoretic studies of the effect of methylglyoxal-bis-guanylhydrazone on blood cells of tumour patients (author's transl)]. The effect of methylglyoxal-bis-guanylhydrazone has been studied on the electrophoretic mobility of blood cells of 10 unselected and untreated patients with preferably solid malignant diseases. Prior to the incubation with CH3-G the electrophoretic mobility of lymphocytes and erythrocytes showed increased and of platelets showed decreased rates which extend over a larger range than usual and for which reasons cannot be given till now. The incubation with CH3-G caused a concentration-dependent reduction of the electrophoretic mobility of all three tested types of blood cells. This can be estimated as an impairment of a function of surface membrane and confirms recently obtained results of activity of CH3-G on platelets' surface membrane.", "PMID": 971046}
{"id": "pubmed23n0032_8334", "title": "[Splenectomy in Hodgkin's disease--indication and results (author's transl)].", "content": "The spleen is involved in 20-30 percent of patients with Hodgkin's disease. Involvement of the spleen cannot be excluded with reliability by radiological procedures or clinical chemistry. Therefore laparotomy and splenectomy are recommended as staging and therapeutic measures. In the authors's material, splenectomy showed splenic lesions in 9 out of 21 patients, mostly unexpected. Splenectomy is always indicated in stage II and should be taken into consideration in individual cases of stages I and III, too.", "contents": "[Splenectomy in Hodgkin's disease--indication and results (author's transl)]. The spleen is involved in 20-30 percent of patients with Hodgkin's disease. Involvement of the spleen cannot be excluded with reliability by radiological procedures or clinical chemistry. Therefore laparotomy and splenectomy are recommended as staging and therapeutic measures. In the authors's material, splenectomy showed splenic lesions in 9 out of 21 patients, mostly unexpected. Splenectomy is always indicated in stage II and should be taken into consideration in individual cases of stages I and III, too.", "PMID": 971047}
{"id": "pubmed23n0032_8335", "title": "[Basal cell carcinoma (basalioma solidum) on a tubed pedicle flap in the oral cavity (author's transl)].", "content": "A report is given on the formation of a basal cell carcinoma (Basalioma solidum) on a tubed pedicle flap of acromiopectoral transplanted into the oral cavity. This plastic successfully covered a vast perforation of the palate after a severe wounding by shell splinters 33 years ago. The histologically proofed basal cell carcinoma (Basalioma solidum) was entirely brought to disappearance by 6000 R tumor-dose telecobalt. After 1 1/2 years the patient was without any complaint and recurrence. Etiology and pathogenesis of basal cell carcinoma (Basalioma solidum) are discussed in the light of the literature.", "contents": "[Basal cell carcinoma (basalioma solidum) on a tubed pedicle flap in the oral cavity (author's transl)]. A report is given on the formation of a basal cell carcinoma (Basalioma solidum) on a tubed pedicle flap of acromiopectoral transplanted into the oral cavity. This plastic successfully covered a vast perforation of the palate after a severe wounding by shell splinters 33 years ago. The histologically proofed basal cell carcinoma (Basalioma solidum) was entirely brought to disappearance by 6000 R tumor-dose telecobalt. After 1 1/2 years the patient was without any complaint and recurrence. Etiology and pathogenesis of basal cell carcinoma (Basalioma solidum) are discussed in the light of the literature.", "PMID": 971048}
{"id": "pubmed23n0032_8336", "title": "[Changes in the exocrine secretion of the pancreas after thyroidectomy. Experimental research].", "content": "Reduction of protein and bicarbonate flow and concentration after experimental thyroidectomy was observed in the rat. Clinical applications of the reduction of thyroid activity in the treatment of acute pancreatitis are proposed.", "contents": "[Changes in the exocrine secretion of the pancreas after thyroidectomy. Experimental research]. Reduction of protein and bicarbonate flow and concentration after experimental thyroidectomy was observed in the rat. Clinical applications of the reduction of thyroid activity in the treatment of acute pancreatitis are proposed.", "PMID": 971079}
{"id": "pubmed23n0032_8337", "title": "Subclavian steal syndrome. A 12-year experience.", "content": "Over a 12-year period, 43 operations were performed on 40 patients for correction of the subclavian steal syndrome. Eleven of these patients also underwent endarterectomy for coexisting carotid artery disease. There were 16 transthoracic procedures and 27 transcervical procedures. Graft failure in two patients was corrected by a second operation. There was one early death and one late death. The remaining 38 patients have been carefully studied for up to 12 years, with an average follow-up period of 60 months. These 38 patients have remained asymptomatic and their revascularization procedures have remained functional, as determined by blood pressure determinations in the involved extremity and, in some cases, by arteriography.", "contents": "Subclavian steal syndrome. A 12-year experience. Over a 12-year period, 43 operations were performed on 40 patients for correction of the subclavian steal syndrome. Eleven of these patients also underwent endarterectomy for coexisting carotid artery disease. There were 16 transthoracic procedures and 27 transcervical procedures. Graft failure in two patients was corrected by a second operation. There was one early death and one late death. The remaining 38 patients have been carefully studied for up to 12 years, with an average follow-up period of 60 months. These 38 patients have remained asymptomatic and their revascularization procedures have remained functional, as determined by blood pressure determinations in the involved extremity and, in some cases, by arteriography.", "PMID": 971084}
{"id": "pubmed23n0032_8338", "title": "Leiomyosarcoma of the inferior vena cava. Diagnosis and surgical management.", "content": "Leiomyosarcoma of vascular origin are rare tumors arising most frequently from the inferior vena cava (IVC). We report on three patients one of whom underwent definitive resection. These tumors most commonly involve the upper segment of the IVC, and appear with manifestations of the Budd-Chiari syndrome. Lesions at this level are not amenable to surgical therapy. Tumors of the middle and lower segments of the IVC usually cause right-sided pain. Diagnosis is difficult, but is best approached preoperatively by angiography and vena cavography. Optimal therapy of lesions at these levels is surgical resection. Resection of the IVC below the hepatic veins is possible with renal function preserved by collateral drainage of the left renal vein.", "contents": "Leiomyosarcoma of the inferior vena cava. Diagnosis and surgical management. Leiomyosarcoma of vascular origin are rare tumors arising most frequently from the inferior vena cava (IVC). We report on three patients one of whom underwent definitive resection. These tumors most commonly involve the upper segment of the IVC, and appear with manifestations of the Budd-Chiari syndrome. Lesions at this level are not amenable to surgical therapy. Tumors of the middle and lower segments of the IVC usually cause right-sided pain. Diagnosis is difficult, but is best approached preoperatively by angiography and vena cavography. Optimal therapy of lesions at these levels is surgical resection. Resection of the IVC below the hepatic veins is possible with renal function preserved by collateral drainage of the left renal vein.", "PMID": 971085}
{"id": "pubmed23n0032_8339", "title": "[Flowmeter changes induced by glucagon in hepato-portal hemodynamics in the course of hemorrhagic shock in dogs].", "content": "Anoxic lesion in haemorrhagic shock may result in exclusion of the capillary circulation to the point of stagnant hypoxia. Under normal conditions, glucagon regulates hepatoportal haemodynamics. Its experimentation in the correction of haemorrhagic shock in the dog showed that it increased hepatoportal flow towards normality by reducing local resistance. The consequences with respect to intestinal tissue metabolism make this finding of particular interest.", "contents": "[Flowmeter changes induced by glucagon in hepato-portal hemodynamics in the course of hemorrhagic shock in dogs]. Anoxic lesion in haemorrhagic shock may result in exclusion of the capillary circulation to the point of stagnant hypoxia. Under normal conditions, glucagon regulates hepatoportal haemodynamics. Its experimentation in the correction of haemorrhagic shock in the dog showed that it increased hepatoportal flow towards normality by reducing local resistance. The consequences with respect to intestinal tissue metabolism make this finding of particular interest.", "PMID": 971078}
{"id": "pubmed23n0032_8340", "title": "Postoperative acute acalculous cholecystitis.", "content": "Two patients with acute acalculous cholecystitis after major surgical operations (cystectomy ad modum Bricker because of carcinoma of the urinary bladder, and proctocolectomy because of ulcerative colitis) are described. Various possible causes of acalculous cholecystitis after operation or after trauma are discussed. Postoperative acute acalculous cholecystitis has a more fulminant course than ordinary calculous cholecystitis. Treatment consists of immediate cholecystectomy.", "contents": "Postoperative acute acalculous cholecystitis. Two patients with acute acalculous cholecystitis after major surgical operations (cystectomy ad modum Bricker because of carcinoma of the urinary bladder, and proctocolectomy because of ulcerative colitis) are described. Various possible causes of acalculous cholecystitis after operation or after trauma are discussed. Postoperative acute acalculous cholecystitis has a more fulminant course than ordinary calculous cholecystitis. Treatment consists of immediate cholecystectomy.", "PMID": 971086}
{"id": "pubmed23n0032_8341", "title": "[Toxicological and pharmacological studies of glycerol].", "content": "The toxicological effects of 10% glycerol were investigated in the mouse and rat. This product displayed very low acute toxicity on i.v. and intraperitoneal administration while no toxic effects were noted in the rat as a result of repeated administration over 3 months. Teratogenic effects were not observed after daily i.v. administration in the rabbit. Changes in arterial pressure and globular osmotic resistance did not appear during or after continuous infusion. The relevant literature is also examined. Glycerol has been found active in experimental various models of cerebral oedema. It also has a useful effect on cerebral metabolism.", "contents": "[Toxicological and pharmacological studies of glycerol]. The toxicological effects of 10% glycerol were investigated in the mouse and rat. This product displayed very low acute toxicity on i.v. and intraperitoneal administration while no toxic effects were noted in the rat as a result of repeated administration over 3 months. Teratogenic effects were not observed after daily i.v. administration in the rabbit. Changes in arterial pressure and globular osmotic resistance did not appear during or after continuous infusion. The relevant literature is also examined. Glycerol has been found active in experimental various models of cerebral oedema. It also has a useful effect on cerebral metabolism.", "PMID": 971082}
{"id": "pubmed23n0032_8342", "title": "Gallstone pancreatitis. Exploration of the biliary system in acute and recurrent pancreatitis.", "content": "During a five-year period, 82 patients were treated for acute pancreatitis, 63 of whom were proved to have associated biliary tract disease. In 18 of the 63, the accepted preoperative diagnostic measures failed to demonstrate pathologic findings in the biliary system. In 16 of the 18 patients, stones were discovered at the time of operation, although in five they were so small as to be demonstrable only filtering the aspirated bile through gauze. In the two of the 18 without stones, cholecystitis was present. In 14 patients the ducts choledochus and the pancreatic duct had a common path. All patients had no further pancreatitis two to eight years cholecystectomy. In Israel, where alcoholism is rare, three fourths of the cases of acute pancreatitis are associated with gallbladder disease.", "contents": "Gallstone pancreatitis. Exploration of the biliary system in acute and recurrent pancreatitis. During a five-year period, 82 patients were treated for acute pancreatitis, 63 of whom were proved to have associated biliary tract disease. In 18 of the 63, the accepted preoperative diagnostic measures failed to demonstrate pathologic findings in the biliary system. In 16 of the 18 patients, stones were discovered at the time of operation, although in five they were so small as to be demonstrable only filtering the aspirated bile through gauze. In the two of the 18 without stones, cholecystitis was present. In 14 patients the ducts choledochus and the pancreatic duct had a common path. All patients had no further pancreatitis two to eight years cholecystectomy. In Israel, where alcoholism is rare, three fourths of the cases of acute pancreatitis are associated with gallbladder disease.", "PMID": 971087}
{"id": "pubmed23n0032_8343", "title": "[Accidental inhalation of a foreign body. Presentation of 2 cases with atypical symptoms].", "content": "Problems related to the inhalation of foreign bodies by children are discussed and two cases with unusual symptom pictures are presented. It is shown that some instances of acute respiratory or cardiorespiratory insufficiency of unknown origin in infancy may in fact be attributable to a foreign body in the respiratory system.", "contents": "[Accidental inhalation of a foreign body. Presentation of 2 cases with atypical symptoms]. Problems related to the inhalation of foreign bodies by children are discussed and two cases with unusual symptom pictures are presented. It is shown that some instances of acute respiratory or cardiorespiratory insufficiency of unknown origin in infancy may in fact be attributable to a foreign body in the respiratory system.", "PMID": 971083}
{"id": "pubmed23n0032_8344", "title": "[Role of respiratory physiokinetic therapy in general surgery].", "content": "The applications of respiratory physiokinesitherapy in general surgery are considered. Emphasis is placed on its importance in the prevention and management of complications venous thrombosis and embolia, in the conservation of muscle strength, and as routine pre- and postoperative management.", "contents": "[Role of respiratory physiokinetic therapy in general surgery]. The applications of respiratory physiokinesitherapy in general surgery are considered. Emphasis is placed on its importance in the prevention and management of complications venous thrombosis and embolia, in the conservation of muscle strength, and as routine pre- and postoperative management.", "PMID": 971080}
{"id": "pubmed23n0032_8345", "title": "Congenital choledochal cysts.", "content": "Cystic dilation of the common bile duct is a rare congenital anomaly. The cases of two patients, 2 1/2 and 18 years old, respectively, illustrate the difficulty in preoperative diagnosis, as well as the value of long-term postoperative follow-up. Ascending cholangitis developed in one of the patients after a Roux-en-Y cystojejunostomy nine years after operation. A follow-up of three years after cystoduodenostomy and 14 years after a hepaticojejunostomy is reported. In infants and small children, a cystoduodenostomy is usually done; otherwise, cystojejunostomy should be the preferred initial operation. Excision may be attempted if the cyst is very small, and in selected cases.", "contents": "Congenital choledochal cysts. Cystic dilation of the common bile duct is a rare congenital anomaly. The cases of two patients, 2 1/2 and 18 years old, respectively, illustrate the difficulty in preoperative diagnosis, as well as the value of long-term postoperative follow-up. Ascending cholangitis developed in one of the patients after a Roux-en-Y cystojejunostomy nine years after operation. A follow-up of three years after cystoduodenostomy and 14 years after a hepaticojejunostomy is reported. In infants and small children, a cystoduodenostomy is usually done; otherwise, cystojejunostomy should be the preferred initial operation. Excision may be attempted if the cyst is very small, and in selected cases.", "PMID": 971088}
{"id": "pubmed23n0032_8346", "title": "Central venous pressure and pulmonary wedge pressure. A comparative study in anesthetized surgical patients.", "content": "To determine the reliability of central venous pressure (CVP) as a guide to fluid therapy during an operation, repeated and simultaneous CVP and pulmonary wedge pressure (PWP) measurements were made with a Swan-Ganz catheter in 13 relatively elderly patients without obvious cardiac or respiratory disease- Overall correlation between CVP and PWP was highly significant (P less than .001); there was, however, an important variation of the correlation for each patient. For values of CVP greater than or equal to 8 mm Hg, the correlation was not significant. The disparity between right and left ventricular filling pressures was confirmed by the relationship between serial changes in CVP and PWP. These data strongly suggest that in relatively elderly patients undergoing surgery without evidence of cardiac or respiratory disease, CVP may be misleading index for appreciating PWP.", "contents": "Central venous pressure and pulmonary wedge pressure. A comparative study in anesthetized surgical patients. To determine the reliability of central venous pressure (CVP) as a guide to fluid therapy during an operation, repeated and simultaneous CVP and pulmonary wedge pressure (PWP) measurements were made with a Swan-Ganz catheter in 13 relatively elderly patients without obvious cardiac or respiratory disease- Overall correlation between CVP and PWP was highly significant (P less than .001); there was, however, an important variation of the correlation for each patient. For values of CVP greater than or equal to 8 mm Hg, the correlation was not significant. The disparity between right and left ventricular filling pressures was confirmed by the relationship between serial changes in CVP and PWP. These data strongly suggest that in relatively elderly patients undergoing surgery without evidence of cardiac or respiratory disease, CVP may be misleading index for appreciating PWP.", "PMID": 971089}
{"id": "pubmed23n0032_8347", "title": "Hemicorporectomy for intractable decubitus ulcers.", "content": "A hemicorporectomy was successfully employed for control of intractable decubitus ulcers, osteomyelitis, and low-grade epidermoid cancer in a 55-year-old man marked reduction in pulmonary functions. Strict attention to postoperative fluid balance was associated with a smooth convalescence. The operation may be more applicable to paraplegic patients with intractable pelvic decubiti than to those with advanced pelvic tumors. In view of the increased number of paraplegics who may be at risk for developing this problem, more frequent consideration of the procedure seems warranted.", "contents": "Hemicorporectomy for intractable decubitus ulcers. A hemicorporectomy was successfully employed for control of intractable decubitus ulcers, osteomyelitis, and low-grade epidermoid cancer in a 55-year-old man marked reduction in pulmonary functions. Strict attention to postoperative fluid balance was associated with a smooth convalescence. The operation may be more applicable to paraplegic patients with intractable pelvic decubiti than to those with advanced pelvic tumors. In view of the increased number of paraplegics who may be at risk for developing this problem, more frequent consideration of the procedure seems warranted.", "PMID": 971090}
{"id": "pubmed23n0032_8348", "title": "Percutaneous adrenal suppression with topically applied corticosteroids.", "content": "Synthetic fluorinated derivatives of cortisone, when topically applied, are absorbed readily and may suppress adrenal function. In the case presented here, large quantities of topically applied triamcinolone acetonide were used in the treatment of atopic dermatitis. This caused a noticeable decrease in the plasma cortisol level. That this was the result of adrenal suppression rather than insufficiency was indicated by a satisfactory response of the plasma cortisol levels to a cosyntropin injection test. In patients with a history of recent and extensive use of topically applied corticosteroids, appropriate diagnostic and therapeutic measures are indicated to avoid postoperative adrenal insufficiency.", "contents": "Percutaneous adrenal suppression with topically applied corticosteroids. Synthetic fluorinated derivatives of cortisone, when topically applied, are absorbed readily and may suppress adrenal function. In the case presented here, large quantities of topically applied triamcinolone acetonide were used in the treatment of atopic dermatitis. This caused a noticeable decrease in the plasma cortisol level. That this was the result of adrenal suppression rather than insufficiency was indicated by a satisfactory response of the plasma cortisol levels to a cosyntropin injection test. In patients with a history of recent and extensive use of topically applied corticosteroids, appropriate diagnostic and therapeutic measures are indicated to avoid postoperative adrenal insufficiency.", "PMID": 971091}
{"id": "pubmed23n0032_8349", "title": "[Studies on methionine metabolism. 1. Studies on general metabolism and body composition].", "content": "19-day trials were carried out with diets containing varying amounts of methionine (0.3%), 0.8%, 1.3%, 2.0%, 2.8%, 5.3%). Diets containing 0.8% methionine were found to have the most favourable effect. Increasing methionine supplementation gradually decreased the food consumption, the daily weight gains and the efficiency of food utilization of the animals. Data on weight gains and food utilization in the \"pair-fed\" groups were more favourable.  A stepwise decline was observed with regard to the absolute quantity of nitrogen retained (mg) as well as with regard to the quotient (%) of the amount of N retained to the amount of N utilized. Studies on the body composition of the animals showed that all those animals consuming large quantities of methionine did not synthesize protein while the process of protein synthesis in \"pair fed\" animals was found to be reduced, but still positive. These results suggest the occurrence of disorders in the protein metabolism arising in the organism of the animals in response to the uptake of large amounts of methionine. The relatively low fat content of the bodies of animals receiving excessive amounts of methionine, the increasing losses of fat during the trial as well as decreases in the RQ values indicated an increased rate of fat utilization in these animals. The relative water content of the body of animals receiving excessive quantities of methionine was higher, but, nevertheless, the massive weight decreases were, in the first instance, due to losses of water. Loss of water and fat as well as relative hydratation are characteristic symptoms of malnutrition. These were, to some extent, also observed in \"pair fed\" animals.", "contents": "[Studies on methionine metabolism. 1. Studies on general metabolism and body composition]. 19-day trials were carried out with diets containing varying amounts of methionine (0.3%), 0.8%, 1.3%, 2.0%, 2.8%, 5.3%). Diets containing 0.8% methionine were found to have the most favourable effect. Increasing methionine supplementation gradually decreased the food consumption, the daily weight gains and the efficiency of food utilization of the animals. Data on weight gains and food utilization in the \"pair-fed\" groups were more favourable.  A stepwise decline was observed with regard to the absolute quantity of nitrogen retained (mg) as well as with regard to the quotient (%) of the amount of N retained to the amount of N utilized. Studies on the body composition of the animals showed that all those animals consuming large quantities of methionine did not synthesize protein while the process of protein synthesis in \"pair fed\" animals was found to be reduced, but still positive. These results suggest the occurrence of disorders in the protein metabolism arising in the organism of the animals in response to the uptake of large amounts of methionine. The relatively low fat content of the bodies of animals receiving excessive amounts of methionine, the increasing losses of fat during the trial as well as decreases in the RQ values indicated an increased rate of fat utilization in these animals. The relative water content of the body of animals receiving excessive quantities of methionine was higher, but, nevertheless, the massive weight decreases were, in the first instance, due to losses of water. Loss of water and fat as well as relative hydratation are characteristic symptoms of malnutrition. These were, to some extent, also observed in \"pair fed\" animals.", "PMID": 971095}
{"id": "pubmed23n0032_8350", "title": "[35S excretion in rats after injection of 35S methionine in relation to protein and amino acid nutrition].", "content": "Experimental rats received purified or semisynthetic diets containing 10% or 6% crude protein (Soya protein). These were supplemented with 0.1% or 0.2% L-, D- or DL methionine. After a 5-day feeding period the animals were injected 35S-DL-methionine. For 48 hrs after 35S administration the pattern of urinary 35S excretion was traced. Neither the level of protein does not the configuration of the methionine supplemented nor the level of methionine supplementation were found to influence urinary 35S excretion in any way. Amino acid imbalance as induced by supplementation of 5% DL threonine produced a highly significant increase in the rate of 35S excretion on the 1st day of experiment. The results obtained suggest the conclusion that any determination of methionine requirements based on metabolic data should leave out from consideration values on the level of urinary 35S excretion obtained on the first day of experiment, as these are dependent on amino acid transport rather than on protein metabolism.", "contents": "[35S excretion in rats after injection of 35S methionine in relation to protein and amino acid nutrition]. Experimental rats received purified or semisynthetic diets containing 10% or 6% crude protein (Soya protein). These were supplemented with 0.1% or 0.2% L-, D- or DL methionine. After a 5-day feeding period the animals were injected 35S-DL-methionine. For 48 hrs after 35S administration the pattern of urinary 35S excretion was traced. Neither the level of protein does not the configuration of the methionine supplemented nor the level of methionine supplementation were found to influence urinary 35S excretion in any way. Amino acid imbalance as induced by supplementation of 5% DL threonine produced a highly significant increase in the rate of 35S excretion on the 1st day of experiment. The results obtained suggest the conclusion that any determination of methionine requirements based on metabolic data should leave out from consideration values on the level of urinary 35S excretion obtained on the first day of experiment, as these are dependent on amino acid transport rather than on protein metabolism.", "PMID": 971096}
{"id": "pubmed23n0032_8351", "title": "[Cu concentration in the milk of rats receiving diets with varying Cu content].", "content": "2 trials were conducted with female rats to investigate in which way the level of dietary Cu influenced the level of Cu concentrations in rat milk. The trials were made to establish whether data on the Cu content of milk might be used as indication of the level and state of Cu supply to the animal. In the period of colostrum formation the Cu content of the milk was shown to be clearly dependent on the level of dietary Cu. In later periods of lactation Cu concentrations in the milk (from 2 mg of Cu per kg of diet) were found to be unaffected by the Cu content of the diet. Constant Cu concentrations of between 2.5 to 3.0 mg per kg of milk were noted in this period. It was only in cases of extreme Cu deficiency (0.5 mg/kg of diet) that soon after parturition the Cu values decreased to and remained at a very low level of 1 mg per kg of milk. Generally, colostrum contained 4-6 times more Cu than ordinary milk (in all groups).", "contents": "[Cu concentration in the milk of rats receiving diets with varying Cu content]. 2 trials were conducted with female rats to investigate in which way the level of dietary Cu influenced the level of Cu concentrations in rat milk. The trials were made to establish whether data on the Cu content of milk might be used as indication of the level and state of Cu supply to the animal. In the period of colostrum formation the Cu content of the milk was shown to be clearly dependent on the level of dietary Cu. In later periods of lactation Cu concentrations in the milk (from 2 mg of Cu per kg of diet) were found to be unaffected by the Cu content of the diet. Constant Cu concentrations of between 2.5 to 3.0 mg per kg of milk were noted in this period. It was only in cases of extreme Cu deficiency (0.5 mg/kg of diet) that soon after parturition the Cu values decreased to and remained at a very low level of 1 mg per kg of milk. Generally, colostrum contained 4-6 times more Cu than ordinary milk (in all groups).", "PMID": 971097}
{"id": "pubmed23n0032_8352", "title": "[Determination of phosphonic acids in ruminal fluid].", "content": "A technique has been worked out for determining amino alkyl phosphonic acids in ruminal fluid by using methods of hydrolysis, partition of the phosphonic acid on a cation exchange column and ashing of the eluate. After evaporation to total dryness the phosphonic acids were determined as phosphates. The amounts of acid found in the ruminal fluid of goats varied between 0.4 and 1.1 muMol/ml. In view of the fact that these acids are microbial compounds it will appear that any change in the amount of compounds produced in the rumen would indicate changes in the concentration of the microbial mass in the rumen.", "contents": "[Determination of phosphonic acids in ruminal fluid]. A technique has been worked out for determining amino alkyl phosphonic acids in ruminal fluid by using methods of hydrolysis, partition of the phosphonic acid on a cation exchange column and ashing of the eluate. After evaporation to total dryness the phosphonic acids were determined as phosphates. The amounts of acid found in the ruminal fluid of goats varied between 0.4 and 1.1 muMol/ml. In view of the fact that these acids are microbial compounds it will appear that any change in the amount of compounds produced in the rumen would indicate changes in the concentration of the microbial mass in the rumen.", "PMID": 971098}
{"id": "pubmed23n0032_8353", "title": "[Influence of milk-fat substitute feeding on the metabolism in calves].", "content": "Investigations were made to study the effect of milk fat substitutes (\"Laktin\", \"TZS-60\") on the metabolism of 3 and 5-weeks-old calves. \"Laktin\" is prepared from lard and hydrogenated soya and fish oil; additionally, it contains lecithin, monoglycerides, antioxidants, vitamins and antibiotics. \"TSZ-60\" is a preparation containing starch and colloidal silicic acid as carrier material mixed with 60% of coarsely powdered fat (particle sizes ranging from 50 to 200 mum), which in turn, is supplemented with vitamins and antibiotics. The kinds of \"Laktin\" used in the present trial contained 95%, 58%-98% or 90% of fat while \"TSZ-60\" contained 59.4%. Both the 3-weeks-old and the 5-weeks-old calves digested the \"Laktin\" fat more efficiently than the kind of fat contained in \"TSZ-60\" (\"Laktin\": 94.3% to 97.3% of fat; \"TSZ-60\" 71.8% to 90.5%). As a consequence of this, the \"Laktin\"-fed calves exhibited a higher rate of nitrogen utilization and nitrogen retention. According to the results obtained in the present trial \"Laktin\" should be given in daily doses of 219 g (an amount corresponding to 7 litres of milk with a milk fat content of 3.13%) while \"TSZ-60\" should be fed in daily amounts of 245 g (corresponding to 7 litres of milk with a milk fat content of 2.1%). Higher doses (\"Laktin\": 323 g/day: \"TSZ-60\" 335 g/day) would have a detrimental effect on the metabolism of the calves. Apart from this, it was not found that higher doses would help to reduce the protein consumption of the animals.", "contents": "[Influence of milk-fat substitute feeding on the metabolism in calves]. Investigations were made to study the effect of milk fat substitutes (\"Laktin\", \"TZS-60\") on the metabolism of 3 and 5-weeks-old calves. \"Laktin\" is prepared from lard and hydrogenated soya and fish oil; additionally, it contains lecithin, monoglycerides, antioxidants, vitamins and antibiotics. \"TSZ-60\" is a preparation containing starch and colloidal silicic acid as carrier material mixed with 60% of coarsely powdered fat (particle sizes ranging from 50 to 200 mum), which in turn, is supplemented with vitamins and antibiotics. The kinds of \"Laktin\" used in the present trial contained 95%, 58%-98% or 90% of fat while \"TSZ-60\" contained 59.4%. Both the 3-weeks-old and the 5-weeks-old calves digested the \"Laktin\" fat more efficiently than the kind of fat contained in \"TSZ-60\" (\"Laktin\": 94.3% to 97.3% of fat; \"TSZ-60\" 71.8% to 90.5%). As a consequence of this, the \"Laktin\"-fed calves exhibited a higher rate of nitrogen utilization and nitrogen retention. According to the results obtained in the present trial \"Laktin\" should be given in daily doses of 219 g (an amount corresponding to 7 litres of milk with a milk fat content of 3.13%) while \"TSZ-60\" should be fed in daily amounts of 245 g (corresponding to 7 litres of milk with a milk fat content of 2.1%). Higher doses (\"Laktin\": 323 g/day: \"TSZ-60\" 335 g/day) would have a detrimental effect on the metabolism of the calves. Apart from this, it was not found that higher doses would help to reduce the protein consumption of the animals.", "PMID": 971099}
{"id": "pubmed23n0032_8354", "title": "[Substitution of protein feed through lysine-supplemented high-protein wheat during the rearing and laying period of hens. 2. Effect of graded lysine doses on food consumption and the rate of weight gain in young hens].", "content": "In a feeding trial 1920 young hens received rations consisting mainly of high-protein wheat with or without additions of lysine or methionine (table 1) and a control ration. The five rations contained 0.77%, 0,59%, 0,61%, 0,54% of 0,46% lysine. In the period between the 9th and 20th week of experiment the hens receiving less lysine consumed less food in the beginning but more food towards the end of this experimental period (12th to 16th week of experiment). The lower energy content of the control diet produced a higher rate of food consumption. Variations in the lysine supply in general produced only slight differences in feed consumption and weight gain in the rearing period of the hens, whereas a compensatory effect was observed with regard to the growth rate of the birds. Despite the balanced crude protein consumption of the birds a positive linear regression of crude protein utilization was calculated for the percantege crude protein retention (y) as depending upon the lysine content of the ration (x) (y = 11.34 + 19,61x; B = 0.93). The wheat protein could be improved by lysine supplements.", "contents": "[Substitution of protein feed through lysine-supplemented high-protein wheat during the rearing and laying period of hens. 2. Effect of graded lysine doses on food consumption and the rate of weight gain in young hens]. In a feeding trial 1920 young hens received rations consisting mainly of high-protein wheat with or without additions of lysine or methionine (table 1) and a control ration. The five rations contained 0.77%, 0,59%, 0,61%, 0,54% of 0,46% lysine. In the period between the 9th and 20th week of experiment the hens receiving less lysine consumed less food in the beginning but more food towards the end of this experimental period (12th to 16th week of experiment). The lower energy content of the control diet produced a higher rate of food consumption. Variations in the lysine supply in general produced only slight differences in feed consumption and weight gain in the rearing period of the hens, whereas a compensatory effect was observed with regard to the growth rate of the birds. Despite the balanced crude protein consumption of the birds a positive linear regression of crude protein utilization was calculated for the percantege crude protein retention (y) as depending upon the lysine content of the ration (x) (y = 11.34 + 19,61x; B = 0.93). The wheat protein could be improved by lysine supplements.", "PMID": 971100}
{"id": "pubmed23n0032_8355", "title": "[Methionine metabolism. 2. Biochemical and histological studies in rats].", "content": "Male rats weighing 100 g were used in a 19-day trial. The rats received diets containing varying proportions of methionine. Their absolute liver weight was lower, due to the high methionine levels, than that of the animals fed optimum rations, whereas the relative liver weight was found to be lower only in the pair-fed group. No essential differences were observed between the levels of dry matter, protein, and fat. Glycogen levels were found to be lowered only in cases when high methionine doses were administered. Pathologically low levels of glycogen were found in animals of the pair-fed group. High methionine consumption reduced the percentages of 12 glucogenetic amino acids in the liver. This, in turn, suggests an increased rate of glucose formation from amino acids, a process which was not observed in animals of the pair-fed group. High methionine doses produced a condition of hyperglycaemia, while in the pair-fed group hypoglycaemia was observed. The highest rate of secretion of thyroid hormones was found in animals receiving the optimum ration; a lower rate of secretion was observed in the animals consuming less or more methionine. In animals receiving high methionine levels the rate of hormone secretion decreased in a stepwise manner with increasing methionine supplementation. Only the highest methionine doses (5,3%) were found to have a negative effect on the maximum rate of iodine incorporation into the thyroid gland. These findings were also substantiated by histological investigations because a true pathological picture was only found in histological sections of thyroid tissue from animals fed the highest methionine doses (5,3%) The thyroid tissue of these animals consisted predominantly of small glandular alveoli whose colloid was slightly coloured. The epithelial cells of these alveoli were degenerated; some cases of liver cell disintegration and, more rarely, cellular necrosis were noted.", "contents": "[Methionine metabolism. 2. Biochemical and histological studies in rats]. Male rats weighing 100 g were used in a 19-day trial. The rats received diets containing varying proportions of methionine. Their absolute liver weight was lower, due to the high methionine levels, than that of the animals fed optimum rations, whereas the relative liver weight was found to be lower only in the pair-fed group. No essential differences were observed between the levels of dry matter, protein, and fat. Glycogen levels were found to be lowered only in cases when high methionine doses were administered. Pathologically low levels of glycogen were found in animals of the pair-fed group. High methionine consumption reduced the percentages of 12 glucogenetic amino acids in the liver. This, in turn, suggests an increased rate of glucose formation from amino acids, a process which was not observed in animals of the pair-fed group. High methionine doses produced a condition of hyperglycaemia, while in the pair-fed group hypoglycaemia was observed. The highest rate of secretion of thyroid hormones was found in animals receiving the optimum ration; a lower rate of secretion was observed in the animals consuming less or more methionine. In animals receiving high methionine levels the rate of hormone secretion decreased in a stepwise manner with increasing methionine supplementation. Only the highest methionine doses (5,3%) were found to have a negative effect on the maximum rate of iodine incorporation into the thyroid gland. These findings were also substantiated by histological investigations because a true pathological picture was only found in histological sections of thyroid tissue from animals fed the highest methionine doses (5,3%) The thyroid tissue of these animals consisted predominantly of small glandular alveoli whose colloid was slightly coloured. The epithelial cells of these alveoli were degenerated; some cases of liver cell disintegration and, more rarely, cellular necrosis were noted.", "PMID": 971101}
{"id": "pubmed23n0032_8356", "title": "[Nitrogen metabolism of broilers fed N-15 labeled wheat. 3. Blood, liver, spleen, thymus, kidney, brain, pancreas, labile N reservoir].", "content": "Studies on the N metabolism of 4-week-old male broilers fed 15N Labelled wheat revealed a close correlation existing between the levels of 15N concentrations in the small intestine and in the plasma NPN fraction of the blood. The highest level of excess 15Natom0/0 in plasma proteins was noted as late as 20-30 hrs after administration of the last tracer dose. The mean life time of plasma proteins in broilers is less than 2.0 days. The rapid and statistically significant decrease of 15N frequency in the liver, spleen, thymus, and kidneys in the period from 12 hrs after withdrawal of the last 15N dose until the end of the trial reflects the intensity of N turnover in these organs. In the period from 6 hrs to 12 hrs after the last N intake the mean quantities of N in the liver, small intestine and kidneys decreased by 18%, 29%, 26% and 16%. The quantity of N accumulated in these organs after food intake is gradually released into the organism.", "contents": "[Nitrogen metabolism of broilers fed N-15 labeled wheat. 3. Blood, liver, spleen, thymus, kidney, brain, pancreas, labile N reservoir]. Studies on the N metabolism of 4-week-old male broilers fed 15N Labelled wheat revealed a close correlation existing between the levels of 15N concentrations in the small intestine and in the plasma NPN fraction of the blood. The highest level of excess 15Natom0/0 in plasma proteins was noted as late as 20-30 hrs after administration of the last tracer dose. The mean life time of plasma proteins in broilers is less than 2.0 days. The rapid and statistically significant decrease of 15N frequency in the liver, spleen, thymus, and kidneys in the period from 12 hrs after withdrawal of the last 15N dose until the end of the trial reflects the intensity of N turnover in these organs. In the period from 6 hrs to 12 hrs after the last N intake the mean quantities of N in the liver, small intestine and kidneys decreased by 18%, 29%, 26% and 16%. The quantity of N accumulated in these organs after food intake is gradually released into the organism.", "PMID": 971102}
{"id": "pubmed23n0032_8357", "title": "[Protein utilization in calves during different periods of liquid feed fattening].", "content": "2 groups of male calves (Braunvieh) each comprising 5 animals with liveweights, at the day of slaughtering, of 58 kg, 99 kg and 155 kg were used in a trial that was carried out to obtain data on the utilization of dry matter, crude protein, crude fat and ash in the bipartite feeding of calves. The nutrient content of the animals was determined by total body analysis. The following values for nutrient retention were obtained in comparative slaughtering trials by using differentiation techniques. No significant differences between the two fattening periods were observed concerning the utilization of total dry matter in the milk substitutes. About 24% of the dietary dry matter were retained. The mean value for the utilization of dietary fat during the whole period of fattening was found to be about 55% provided, the fat was not used as energy source to meet maintenance requirements. 49% of the milk proteins were utilized for both retention and maintenance in the first period of fattening and 55% during the second period. Differences between the two periods showed no consistent trend. In view of the fact that for all animals used in the trial digestibility was taken to be 95% the utilization coefficients for the digested crude protein were higher by about 3% compared with the figures for crude protein. After subtraction of maintenance needs it was found that, on an average, 63% of the residual amount of digested protein available over the whole fattening period were retained in the body. 55% to 57% of the retained protein were deposited in those parts of the body of the animals that are used for human nutrition. It is for this reason that in the first half of the fattening period only 29.8% of the ingested dietary proteins were retained in the edible cuts of the animal body, as compared with 30.4% during the second half of the fattening period.", "contents": "[Protein utilization in calves during different periods of liquid feed fattening]. 2 groups of male calves (Braunvieh) each comprising 5 animals with liveweights, at the day of slaughtering, of 58 kg, 99 kg and 155 kg were used in a trial that was carried out to obtain data on the utilization of dry matter, crude protein, crude fat and ash in the bipartite feeding of calves. The nutrient content of the animals was determined by total body analysis. The following values for nutrient retention were obtained in comparative slaughtering trials by using differentiation techniques. No significant differences between the two fattening periods were observed concerning the utilization of total dry matter in the milk substitutes. About 24% of the dietary dry matter were retained. The mean value for the utilization of dietary fat during the whole period of fattening was found to be about 55% provided, the fat was not used as energy source to meet maintenance requirements. 49% of the milk proteins were utilized for both retention and maintenance in the first period of fattening and 55% during the second period. Differences between the two periods showed no consistent trend. In view of the fact that for all animals used in the trial digestibility was taken to be 95% the utilization coefficients for the digested crude protein were higher by about 3% compared with the figures for crude protein. After subtraction of maintenance needs it was found that, on an average, 63% of the residual amount of digested protein available over the whole fattening period were retained in the body. 55% to 57% of the retained protein were deposited in those parts of the body of the animals that are used for human nutrition. It is for this reason that in the first half of the fattening period only 29.8% of the ingested dietary proteins were retained in the edible cuts of the animal body, as compared with 30.4% during the second half of the fattening period.", "PMID": 971103}
{"id": "pubmed23n0032_8358", "title": "[Determination of the true digestibility of amino acids in pigs].", "content": "Pigs of varying liveweight received protein-free diets in a trial made to establish the excretion levels of endogenic intestinal nitrogen and endogenic intestinal amino acids. The highest excretion rates of endogenic intestinal N and intestinal amino acids per kg of liveweight or per 100 g of dry matter ingested were found in pigs weighing about 10 to 40 kg. The lowest values were abtained in pigs with a liveweight exceeding 40 kg. The levels of endogenic intestinal N and intestinal amino acids are variable quantities. So, true amino acid digestibility data should be determined in pigs weighing more than 40 kg, because in these animals the relation between the intaken of amino acids and the level of endogenic excretion in the faeces is widest and hence, this variable factor would have the least influence on calculations of digestibility data. The data established for the true digestibility of nitrogen and amino acids of barley fed to pigs weighting 10-100 kg were found to be at the same level (with the exception of lysine) when due consideration was given to figures for the excretion of endogenic intestinal N and intestinal amino acids taking into account the liveweight of the animals.", "contents": "[Determination of the true digestibility of amino acids in pigs]. Pigs of varying liveweight received protein-free diets in a trial made to establish the excretion levels of endogenic intestinal nitrogen and endogenic intestinal amino acids. The highest excretion rates of endogenic intestinal N and intestinal amino acids per kg of liveweight or per 100 g of dry matter ingested were found in pigs weighing about 10 to 40 kg. The lowest values were abtained in pigs with a liveweight exceeding 40 kg. The levels of endogenic intestinal N and intestinal amino acids are variable quantities. So, true amino acid digestibility data should be determined in pigs weighing more than 40 kg, because in these animals the relation between the intaken of amino acids and the level of endogenic excretion in the faeces is widest and hence, this variable factor would have the least influence on calculations of digestibility data. The data established for the true digestibility of nitrogen and amino acids of barley fed to pigs weighting 10-100 kg were found to be at the same level (with the exception of lysine) when due consideration was given to figures for the excretion of endogenic intestinal N and intestinal amino acids taking into account the liveweight of the animals.", "PMID": 971104}
{"id": "pubmed23n0032_8359", "title": "[Effect of zinc deficiency on the digestibility and utilization of nutrients].", "content": "Weaned male rats were used in a metabolic trial to investigate the digestibility of dry matter, crude protein, ash and energy, the energy and N balances and the rates of zinc absorption and zinc retention under conditions of zinc deficiency. 2X8 experimental animals were kept in separate metabolism cages for 6 periods each lasting 5 days. The animals received a semisynthetic zinc-deficient diet (casein + starch + sucrose) containing only 1.0 mg Zn per kg of dry matter (zinc-deficient group fed ad libitum) or plus 49 mg Zn per kg of dry matter (pair-fed control group). The mean intake of dry matter was 2-3 g per day/animal. 2.9 g of food were necessary to produce 1 g of weight increase in the deficient animals and 1.4 g in the controls. The rate of apparent zinc absorption was always clearly negative in the Zn-deficient group. In the control group apparent zinc absorption decreased from 60% to 27% in the course of the trial while zinc retention decreased from 51% to 10%. During zinc deficiency the rates of both faecal and absolute renal Zn excretion were found to be greatly reduced. The proportion of renal zinc excretion relative to total excretion averaged 60% in the experimental group as compared to 17% in the controls. No significant differences were found in the digestibility data of gross energy. Nutrient digestibility (%) was significantly reduced under conditions of Zn deficiency (data for the control group given in brackets): 92,6 (94.0) for dry matter; 93,2 (94.1) for organic matter; 93.3 (96.2) for crude protein and 76.6 (90.8) for crude ash. Similarly, N retention data, in Zn-deficient animals, declined from 30.5% to 14.7%, and metabolizable energy decreased from 88.7% to 85.6%. The differences obtained are not sufficient, however, to account for the clearly increased food requirements observed in zinc-deficient animals. Thus, considerable disturbances have to be assumed to occur in the processes of intermediary nutrient utilization.", "contents": "[Effect of zinc deficiency on the digestibility and utilization of nutrients]. Weaned male rats were used in a metabolic trial to investigate the digestibility of dry matter, crude protein, ash and energy, the energy and N balances and the rates of zinc absorption and zinc retention under conditions of zinc deficiency. 2X8 experimental animals were kept in separate metabolism cages for 6 periods each lasting 5 days. The animals received a semisynthetic zinc-deficient diet (casein + starch + sucrose) containing only 1.0 mg Zn per kg of dry matter (zinc-deficient group fed ad libitum) or plus 49 mg Zn per kg of dry matter (pair-fed control group). The mean intake of dry matter was 2-3 g per day/animal. 2.9 g of food were necessary to produce 1 g of weight increase in the deficient animals and 1.4 g in the controls. The rate of apparent zinc absorption was always clearly negative in the Zn-deficient group. In the control group apparent zinc absorption decreased from 60% to 27% in the course of the trial while zinc retention decreased from 51% to 10%. During zinc deficiency the rates of both faecal and absolute renal Zn excretion were found to be greatly reduced. The proportion of renal zinc excretion relative to total excretion averaged 60% in the experimental group as compared to 17% in the controls. No significant differences were found in the digestibility data of gross energy. Nutrient digestibility (%) was significantly reduced under conditions of Zn deficiency (data for the control group given in brackets): 92,6 (94.0) for dry matter; 93,2 (94.1) for organic matter; 93.3 (96.2) for crude protein and 76.6 (90.8) for crude ash. Similarly, N retention data, in Zn-deficient animals, declined from 30.5% to 14.7%, and metabolizable energy decreased from 88.7% to 85.6%. The differences obtained are not sufficient, however, to account for the clearly increased food requirements observed in zinc-deficient animals. Thus, considerable disturbances have to be assumed to occur in the processes of intermediary nutrient utilization.", "PMID": 971105}
{"id": "pubmed23n0032_8360", "title": "[Constant level of intestinal amino acids as a function of the composition of same].", "content": "The present study was conducted with pigs. It was designed to investigate whether extreme amino acid proportions in the ration would influence the excretion of endogenic amino acids in the faeces. Addition of DL-methionine or L-Lysine produced slight changes in the composition of endogenic intestiornal protein, a fact that was practically of little or no importance for the method of faecal analysis used in determining the true digestibility of amino acids (classical technique).", "contents": "[Constant level of intestinal amino acids as a function of the composition of same]. The present study was conducted with pigs. It was designed to investigate whether extreme amino acid proportions in the ration would influence the excretion of endogenic amino acids in the faeces. Addition of DL-methionine or L-Lysine produced slight changes in the composition of endogenic intestiornal protein, a fact that was practically of little or no importance for the method of faecal analysis used in determining the true digestibility of amino acids (classical technique).", "PMID": 971106}
{"id": "pubmed23n0032_8361", "title": "[Determination of urea in the ruminal fluid of sheep].", "content": "Low values for urea were found in samples of ruminal fluid of sheep fed concentrate rations if urea determination was carried out by the urease method. The present paper describes a both simple and sensitive colorimetric method of analysis using diacetyldioxime. The method is suitable for determining urea in coloured solutions such as ruminal fluid or silage extracts.", "contents": "[Determination of urea in the ruminal fluid of sheep]. Low values for urea were found in samples of ruminal fluid of sheep fed concentrate rations if urea determination was carried out by the urease method. The present paper describes a both simple and sensitive colorimetric method of analysis using diacetyldioxime. The method is suitable for determining urea in coloured solutions such as ruminal fluid or silage extracts.", "PMID": 971107}
{"id": "pubmed23n0032_8362", "title": "[Interaction of nickel with iron, copper and zinc].", "content": "Studies were carried out investigating the role of nickel as an essential element in the growth of rats. Reduced levels of iron, copper and zinc were found in the liver, spleen and kidneys during Ni deficiency. These findings could be reproduced in 2 generations of 30-day and 50-day-old rats. Reduced rates of Fe storage during Ni deficiency were, in some cases, even noted of the Fe supply was raised to a level of as high as 100 mg Fe per kg of the diet. This accounted for the diminished haemoglobin values, the reduced erythrocyte count and reduced hematocrite found under conditions of Ni deficiency.", "contents": "[Interaction of nickel with iron, copper and zinc]. Studies were carried out investigating the role of nickel as an essential element in the growth of rats. Reduced levels of iron, copper and zinc were found in the liver, spleen and kidneys during Ni deficiency. These findings could be reproduced in 2 generations of 30-day and 50-day-old rats. Reduced rates of Fe storage during Ni deficiency were, in some cases, even noted of the Fe supply was raised to a level of as high as 100 mg Fe per kg of the diet. This accounted for the diminished haemoglobin values, the reduced erythrocyte count and reduced hematocrite found under conditions of Ni deficiency.", "PMID": 971108}
{"id": "pubmed23n0032_8363", "title": "[Nitrogen metabolism of broilers fed 15-N labeled wheat. 4. 15-N concentration in muscles and nitrogen utilization].", "content": "Broilers were fed 15N labelled wheat and the concentration of the labelled isotope in muscular tissue was determined. A negative correlation was found to exist between the percentage proportion of N in the muscle fractions and the level of 15N frequency established 12 hrs after administration of the labelled ration. Higher rates of tracer incorporation were observed in the muscles of legs and other muscles (predominatly red muscles) than in pectoral muscles (white muscles). The heart was shown to exhibit the lowest N content and the highest level of 15N frequency of all muscle fractions. In view of the fact that some specific difficulties will arise when certain factors (trace elements, vitamins, ergotropic substances, genetic factors) which influence the N metabolism of fattening poultry, are estimated on the basis of the conventional N balance trials the present tracer technique is recommended for use as an alternative method in studies of the kind just described. In the present trial 20% of the wheat N were utilized for the production of meat N in muscular tissue.", "contents": "[Nitrogen metabolism of broilers fed 15-N labeled wheat. 4. 15-N concentration in muscles and nitrogen utilization]. Broilers were fed 15N labelled wheat and the concentration of the labelled isotope in muscular tissue was determined. A negative correlation was found to exist between the percentage proportion of N in the muscle fractions and the level of 15N frequency established 12 hrs after administration of the labelled ration. Higher rates of tracer incorporation were observed in the muscles of legs and other muscles (predominatly red muscles) than in pectoral muscles (white muscles). The heart was shown to exhibit the lowest N content and the highest level of 15N frequency of all muscle fractions. In view of the fact that some specific difficulties will arise when certain factors (trace elements, vitamins, ergotropic substances, genetic factors) which influence the N metabolism of fattening poultry, are estimated on the basis of the conventional N balance trials the present tracer technique is recommended for use as an alternative method in studies of the kind just described. In the present trial 20% of the wheat N were utilized for the production of meat N in muscular tissue.", "PMID": 971109}
{"id": "pubmed23n0032_8364", "title": "[Distribution of radioactivity in the body and rate of incorporation of radioactivity into the tissue proteins of monogastric animals following intravenous injection of tracer amino acids].", "content": "The studies were carried out with pigs and rats. The radioactive animo acids (14C leucine and 3H lysine) were administered to the pigs by way of a catheter tube into the jugular vein. Subsequently, the time pattern of the distribution of the specific amino acid radioactivity was followed in the TCE soluble and Tce precipitable fractions of the blood plasma (TCE= trichloro-acetic acid). The radioactive labelling in rats was carried out by injecting 14C leucine into the portal vein. The animals were killed after incorporation periods from 2 to 60 mins, and the levels of specific radioactivity were estimated in the TCE soluble and TCE precipitable fractions of the blood plasma, in the liver and in the skeletal muscles. The experimental results clearly indicated that the specific radioactivity of the tracer amino acids and the rate of incorporation of radioactivity into tissue proteins were greatly influenced by the size of the free amino acid pool within the range of distribution of the tracer. An estimation of the magnitude of the pool of free amino acids within the distribution range of the tracer can be obtained from the curve pattern for the decline of specific radioactivity of the corresponding free amino acid in the blood plasma. This pool exhibits a high rate of turnover. In all studies made to evaluate in vivo processes of protein synthesis by use of radioactive tracer amino acids it will be particularly important that consideration should be given to the specific radioactivity of the amino acid in the precursor pool for protein synthesis.", "contents": "[Distribution of radioactivity in the body and rate of incorporation of radioactivity into the tissue proteins of monogastric animals following intravenous injection of tracer amino acids]. The studies were carried out with pigs and rats. The radioactive animo acids (14C leucine and 3H lysine) were administered to the pigs by way of a catheter tube into the jugular vein. Subsequently, the time pattern of the distribution of the specific amino acid radioactivity was followed in the TCE soluble and Tce precipitable fractions of the blood plasma (TCE= trichloro-acetic acid). The radioactive labelling in rats was carried out by injecting 14C leucine into the portal vein. The animals were killed after incorporation periods from 2 to 60 mins, and the levels of specific radioactivity were estimated in the TCE soluble and TCE precipitable fractions of the blood plasma, in the liver and in the skeletal muscles. The experimental results clearly indicated that the specific radioactivity of the tracer amino acids and the rate of incorporation of radioactivity into tissue proteins were greatly influenced by the size of the free amino acid pool within the range of distribution of the tracer. An estimation of the magnitude of the pool of free amino acids within the distribution range of the tracer can be obtained from the curve pattern for the decline of specific radioactivity of the corresponding free amino acid in the blood plasma. This pool exhibits a high rate of turnover. In all studies made to evaluate in vivo processes of protein synthesis by use of radioactive tracer amino acids it will be particularly important that consideration should be given to the specific radioactivity of the amino acid in the precursor pool for protein synthesis.", "PMID": 971110}
{"id": "pubmed23n0032_8365", "title": "[Concepts of G. A. Shmidt concerning the principles of ecologic embryology and their use in current research].", "content": "Under consideration are principle conceptions of G. A. Shmidt on the ecological method in embryology: succession between the compared species, difference in the ecology and organization of adult individuals, distinctions in the course of embryogenesis. Difficulties in a strict observation of all these principles are analyzed in comparative embryological investigations. Possible use of the above principles for studying the intrespecies variability of ontogenetical processes is shown.", "contents": "[Concepts of G. A. Shmidt concerning the principles of ecologic embryology and their use in current research]. Under consideration are principle conceptions of G. A. Shmidt on the ecological method in embryology: succession between the compared species, difference in the ecology and organization of adult individuals, distinctions in the course of embryogenesis. Difficulties in a strict observation of all these principles are analyzed in comparative embryological investigations. Possible use of the above principles for studying the intrespecies variability of ontogenetical processes is shown.", "PMID": 971112}
{"id": "pubmed23n0032_8366", "title": "Infection of newborn and fetal hamsters induced by inoculation of LuIII parvovirus.", "content": "The LuIII parvovirus was adapted to the newborn hamster and produced a systemic infection with massive intestinal hemorrhage. Inoculation of pregnant hamsters lead to transplacental infection of the fetuses and abortion. Most fetal deaths were observed in animals inoculated on days 8 and 10 of gestation. Virus was recovered from dead fetuses, placentas, and viable fetuses. Histological lesions were found in the heart, liver, kidney and CNS of infected fetuses.", "contents": "Infection of newborn and fetal hamsters induced by inoculation of LuIII parvovirus. The LuIII parvovirus was adapted to the newborn hamster and produced a systemic infection with massive intestinal hemorrhage. Inoculation of pregnant hamsters lead to transplacental infection of the fetuses and abortion. Most fetal deaths were observed in animals inoculated on days 8 and 10 of gestation. Virus was recovered from dead fetuses, placentas, and viable fetuses. Histological lesions were found in the heart, liver, kidney and CNS of infected fetuses.", "PMID": 971111}
{"id": "pubmed23n0032_8367", "title": "[Human placental morphology as an index of features of maternal-fetal relationships].", "content": "Principle possibilities of the complex evaluation of the mother-fetus exchange were studied by means of morphological investigations of the human placenta in normality, in vesicular mole, late toxicoses, hemolytic disease of newborns and malformations of the fetus. The investigation of the ratio of the villi of different size, deposition of fibrinoid on their surface, the change of the amount of cytotrophoblastic elements, cells of Kaschenko-Hofbauer and the polymerity of the basic substance are of diagnostic value. The authors propose the basal lamina of the placenta to be included into the scheme of the examination of placenta.", "contents": "[Human placental morphology as an index of features of maternal-fetal relationships]. Principle possibilities of the complex evaluation of the mother-fetus exchange were studied by means of morphological investigations of the human placenta in normality, in vesicular mole, late toxicoses, hemolytic disease of newborns and malformations of the fetus. The investigation of the ratio of the villi of different size, deposition of fibrinoid on their surface, the change of the amount of cytotrophoblastic elements, cells of Kaschenko-Hofbauer and the polymerity of the basic substance are of diagnostic value. The authors propose the basal lamina of the placenta to be included into the scheme of the examination of placenta.", "PMID": 971113}
{"id": "pubmed23n0032_8368", "title": "[Morphofunctional specialization of tissue components of the mammalian placenta and adaptational value in changes in the rate of their differentiation (evolutionary and clinical aspects)].", "content": "It has been shown that in the ontogenesis of human placenta there occur regular changes in the percentage of the villi of various calibre having different interrelationships between the trophoblast, the connective tissue stroma and the fetal vessel. The changes of the number of chorial villi affected by fibrinoid and containing blood vessels under the trophoblast in late toxicoses of women and the anemic syndrom are analyzed (treated and not treated groups of women suffering from different forms of toxicosis were compared). An increased amount of chorial villi having blood vessels under the trophoblast is noted which is a morphofunctional feature of compensatory-adaptational alterations. Possible ecologo-evolutionary approaches to clearing the biological significance of adaptational changes of morphogenesis of the human placenta in pathological pregnancy are shown by the example of the analysis of morphological peculiarities of the placenta of the dolphin.", "contents": "[Morphofunctional specialization of tissue components of the mammalian placenta and adaptational value in changes in the rate of their differentiation (evolutionary and clinical aspects)]. It has been shown that in the ontogenesis of human placenta there occur regular changes in the percentage of the villi of various calibre having different interrelationships between the trophoblast, the connective tissue stroma and the fetal vessel. The changes of the number of chorial villi affected by fibrinoid and containing blood vessels under the trophoblast in late toxicoses of women and the anemic syndrom are analyzed (treated and not treated groups of women suffering from different forms of toxicosis were compared). An increased amount of chorial villi having blood vessels under the trophoblast is noted which is a morphofunctional feature of compensatory-adaptational alterations. Possible ecologo-evolutionary approaches to clearing the biological significance of adaptational changes of morphogenesis of the human placenta in pathological pregnancy are shown by the example of the analysis of morphological peculiarities of the placenta of the dolphin.", "PMID": 971114}
{"id": "pubmed23n0032_8369", "title": "[Localization of the fluorescent part of the Y-chromosome in nuclei of the chorionic symplast of the human placenta].", "content": "The localization of fluorescing Y-chromatin was studied in the nuclei of the chorionic symplast of the mature human placenta in cytological smears and histological sections stained with acrichin-mustard. Its localization within the nucleus and its relationship with nucleoli and nuclear membrane are shown. The significance of studying Y-chromatin in the placenta for population investigations in human cytogenetics and for the establishing of the affiliation of placenta structures is discussed.", "contents": "[Localization of the fluorescent part of the Y-chromosome in nuclei of the chorionic symplast of the human placenta]. The localization of fluorescing Y-chromatin was studied in the nuclei of the chorionic symplast of the mature human placenta in cytological smears and histological sections stained with acrichin-mustard. Its localization within the nucleus and its relationship with nucleoli and nuclear membrane are shown. The significance of studying Y-chromatin in the placenta for population investigations in human cytogenetics and for the establishing of the affiliation of placenta structures is discussed.", "PMID": 971116}
{"id": "pubmed23n0032_8370", "title": "[A previously unknown nucleus in the lateral loop of the dolphin brain, its morphologic organization and origin].", "content": "An earlier unknown cell formation in the lateral loop of the dolphin brain has been first shown and studied. On the basis of cytoarchitectonical and topographical characteristics its homology with the corresponding nucleus of other mammals has been established. The investigation has brought a substantial correction to the morphology of nuclei of the lateral loop of dolphins since the formation described proved to be a ventral nucleus of the lateral loop while the structure known in the literature under this name represents an intermediate nucleus of the lateral loop of the animals concerned.", "contents": "[A previously unknown nucleus in the lateral loop of the dolphin brain, its morphologic organization and origin]. An earlier unknown cell formation in the lateral loop of the dolphin brain has been first shown and studied. On the basis of cytoarchitectonical and topographical characteristics its homology with the corresponding nucleus of other mammals has been established. The investigation has brought a substantial correction to the morphology of nuclei of the lateral loop of dolphins since the formation described proved to be a ventral nucleus of the lateral loop while the structure known in the literature under this name represents an intermediate nucleus of the lateral loop of the animals concerned.", "PMID": 971117}
{"id": "pubmed23n0032_8371", "title": "[Projections of several intralaminar nuclei of the thalamus to the caudate nucleus in cats].", "content": "Direct connections of the central medial, parafascicular and central lateral nuclei of the thalamus with the rostral and caudal parts of the head and the body of the caudate nucleus were studied. The study has revealed that only the central nucleus of the three concerned intralaminar nuclei of the thalamus is projected on the caudate nucleus, head and body. The central medial and parafascicular nucleus of the thalamus does not seem to have direct connections with the caudate nucleus.", "contents": "[Projections of several intralaminar nuclei of the thalamus to the caudate nucleus in cats]. Direct connections of the central medial, parafascicular and central lateral nuclei of the thalamus with the rostral and caudal parts of the head and the body of the caudate nucleus were studied. The study has revealed that only the central nucleus of the three concerned intralaminar nuclei of the thalamus is projected on the caudate nucleus, head and body. The central medial and parafascicular nucleus of the thalamus does not seem to have direct connections with the caudate nucleus.", "PMID": 971118}
{"id": "pubmed23n0032_8372", "title": "[Localization of cells connected with different tropic functions in the hypophysis of the young of Huso huso L. X Acipenser ruthenus L., Chondrostei].", "content": "A number of histological and histochemical methods were used in order to detect different secretory cells in the hypophysis of young Huso huso L. x Acipenser ruthenus L., Chondrostei. special attention was given to somatotropic cells. For their detection the following staining methods were used: orange G - acidic red 2C - acidic green G after fixation in the mixture of HgCl2 with formalin. In the dorsal part of the hypophysis having the appearance of vertical folds pressed into the hypophysis from the dorsal side, the cellular elements are represented mainly by somatotropic cells. In the dorso-nasal part the prolactin cells form the follicles. Part of prolactin cells lie between the follicles. Here there are some corticotropic cells. In the ventral part a considerable amount of basophils are found. The anatomic structure of the hypophysis is considered basing on the above data.", "contents": "[Localization of cells connected with different tropic functions in the hypophysis of the young of Huso huso L. X Acipenser ruthenus L., Chondrostei]. A number of histological and histochemical methods were used in order to detect different secretory cells in the hypophysis of young Huso huso L. x Acipenser ruthenus L., Chondrostei. special attention was given to somatotropic cells. For their detection the following staining methods were used: orange G - acidic red 2C - acidic green G after fixation in the mixture of HgCl2 with formalin. In the dorsal part of the hypophysis having the appearance of vertical folds pressed into the hypophysis from the dorsal side, the cellular elements are represented mainly by somatotropic cells. In the dorso-nasal part the prolactin cells form the follicles. Part of prolactin cells lie between the follicles. Here there are some corticotropic cells. In the ventral part a considerable amount of basophils are found. The anatomic structure of the hypophysis is considered basing on the above data.", "PMID": 971119}
{"id": "pubmed23n0032_8373", "title": "[Morphofunctional analysis of the response of the parotid gland to disruption of its sympathetic innervation].", "content": "The parotid salivary gland of rats was studied after extirpation of the upper cervical sympathetic ganglion in different time after a single feeding. The gland of non-fed animals served as control. The desympathization of the gland has been shown to result in considerable alterations in the ultrastructure of its acinar cells and less pronounced alterations in the salivary tube cells. The interferometrical study has shown that after desympathization the dry weight of acinar cells, the sparseness of the dry weight figures in different animals and the amplitude of the protein amount fluctuations during the secretion cycle decreased. The obtained data point out that to sustain the structural entity of the organ and its normal functioning the sympathetic innervation of the organ must be kept intact.", "contents": "[Morphofunctional analysis of the response of the parotid gland to disruption of its sympathetic innervation]. The parotid salivary gland of rats was studied after extirpation of the upper cervical sympathetic ganglion in different time after a single feeding. The gland of non-fed animals served as control. The desympathization of the gland has been shown to result in considerable alterations in the ultrastructure of its acinar cells and less pronounced alterations in the salivary tube cells. The interferometrical study has shown that after desympathization the dry weight of acinar cells, the sparseness of the dry weight figures in different animals and the amplitude of the protein amount fluctuations during the secretion cycle decreased. The obtained data point out that to sustain the structural entity of the organ and its normal functioning the sympathetic innervation of the organ must be kept intact.", "PMID": 971120}
{"id": "pubmed23n0032_8374", "title": "[Patterns characterizing different levels of animal organization].", "content": "The article deals with features of the regularities characterizing different levels of organization of the animal body. The cell is not only an integrated but also indivisible organic integrity which is due to the  interaction of the nucleus and cytoplasm determining all sides of its vital activity. From this viewpoint the results of experiments on transplantation of the embryo cell nucleus into an enucleated egg of amphibia and the data on somatic hybridization are estimated. All processes of the cell life, their growth, multiplication and differentiation develop within the tissue system and are determined by the regularities of its development. The integrated system of the organism of a multicellular animal unites all the forms of integration of multi-step systemic pattern at different organization levels. Among different categories the systems of tissues and their interaction within the organ are most integrated. The regularities of the tissue development can not be brough to the regularities of the development of the anatomical structure of organs. The integration of cell elements in a tissue system and intertissue relations is the basis of all forms of interdependence in the cell development and the organism structure.", "contents": "[Patterns characterizing different levels of animal organization]. The article deals with features of the regularities characterizing different levels of organization of the animal body. The cell is not only an integrated but also indivisible organic integrity which is due to the  interaction of the nucleus and cytoplasm determining all sides of its vital activity. From this viewpoint the results of experiments on transplantation of the embryo cell nucleus into an enucleated egg of amphibia and the data on somatic hybridization are estimated. All processes of the cell life, their growth, multiplication and differentiation develop within the tissue system and are determined by the regularities of its development. The integrated system of the organism of a multicellular animal unites all the forms of integration of multi-step systemic pattern at different organization levels. Among different categories the systems of tissues and their interaction within the organ are most integrated. The regularities of the tissue development can not be brough to the regularities of the development of the anatomical structure of organs. The integration of cell elements in a tissue system and intertissue relations is the basis of all forms of interdependence in the cell development and the organism structure.", "PMID": 971121}
{"id": "pubmed23n0032_8375", "title": "[Ultrastructure and dynamics of the transosomes of the follicular epithelium of avian ovaries].", "content": "Specific organelles in follicular cells of the avian ovaries - so called transosomes - are described. Transosomes are formed in the follicular cell cytoplasm under the cytoplasmic membrane and consist of a dense layer 100-150 A in thickness and granules 110-250 A in diameter evenly distributed on its inner surface and containing r-RNA. The main property of transosomes is to move from one follicular cell to another or to an oocyte having separated from numerous processes of follicular cells and being surrounded by two additional membranes. In the ooplasm the transosome takes part in the development of primordial yolk granules. It is supposed that transosomes bring about a transmission of somatic follicular cells to the occyte which is necessary for the initial stages of vitellogenesis.", "contents": "[Ultrastructure and dynamics of the transosomes of the follicular epithelium of avian ovaries]. Specific organelles in follicular cells of the avian ovaries - so called transosomes - are described. Transosomes are formed in the follicular cell cytoplasm under the cytoplasmic membrane and consist of a dense layer 100-150 A in thickness and granules 110-250 A in diameter evenly distributed on its inner surface and containing r-RNA. The main property of transosomes is to move from one follicular cell to another or to an oocyte having separated from numerous processes of follicular cells and being surrounded by two additional membranes. In the ooplasm the transosome takes part in the development of primordial yolk granules. It is supposed that transosomes bring about a transmission of somatic follicular cells to the occyte which is necessary for the initial stages of vitellogenesis.", "PMID": 971122}
{"id": "pubmed23n0032_8376", "title": "Ultrastructure of the stroma of nasal polyps: cilia in stromal fibroblasts.", "content": "Ultrastructural examination of the stroma of simple nasal polyps was shown that there is an edematous framework of fibroblasts with abundant interdigitating cytoplasmic processes, a plexus of inflamed blood vessels, and a mixed infiltrate of active inflammatory cells. Solitary cilia were seen to arise from the stromal mesenchymal cells; no mycoplasma or viral inclusions were present. These features may have a relationship to the pathogenesis of nasal polyps. An immunologically induced liberation of vasoactive amines from mast cells results in increased capillary permeability and a consequent excess of intracellular fluid; the stromal cells attempt to check this expansion by an increase in collagen synthesis.", "contents": "Ultrastructure of the stroma of nasal polyps: cilia in stromal fibroblasts. Ultrastructural examination of the stroma of simple nasal polyps was shown that there is an edematous framework of fibroblasts with abundant interdigitating cytoplasmic processes, a plexus of inflamed blood vessels, and a mixed infiltrate of active inflammatory cells. Solitary cilia were seen to arise from the stromal mesenchymal cells; no mycoplasma or viral inclusions were present. These features may have a relationship to the pathogenesis of nasal polyps. An immunologically induced liberation of vasoactive amines from mast cells results in increased capillary permeability and a consequent excess of intracellular fluid; the stromal cells attempt to check this expansion by an increase in collagen synthesis.", "PMID": 971130}
{"id": "pubmed23n0032_8377", "title": "Lymphocyte response in patients with head and neck cancer: effect of clinical stage and radiotherapy.", "content": "Ffty-three patients with head and neck cancer tested before radiation treatment to determine numbers of blood lymphocytes and immunologic responses to mitogens of lymphocytes in whole-blood cultures had mean values that were 19% to 26% less than values for healthy individuals. Thirty patients whose disease was in stages III or IV had values similar to those in 23 patients whose disease was in stages I or II. Values for 45 patients tested at end of radiotherapy decreased to about 50% of pretreatment values; however, patients with advanced lesions experienced greater decreases (to 24% to 50%) than patients with localized lesions (to 71% to 84%). Patients with advanced lesions usually received radiation to larger areas than patients with localized lesions; therefore, the extent of decline in laboratory values was most likely dependent on volume of tissue treated.", "contents": "Lymphocyte response in patients with head and neck cancer: effect of clinical stage and radiotherapy. Ffty-three patients with head and neck cancer tested before radiation treatment to determine numbers of blood lymphocytes and immunologic responses to mitogens of lymphocytes in whole-blood cultures had mean values that were 19% to 26% less than values for healthy individuals. Thirty patients whose disease was in stages III or IV had values similar to those in 23 patients whose disease was in stages I or II. Values for 45 patients tested at end of radiotherapy decreased to about 50% of pretreatment values; however, patients with advanced lesions experienced greater decreases (to 24% to 50%) than patients with localized lesions (to 71% to 84%). Patients with advanced lesions usually received radiation to larger areas than patients with localized lesions; therefore, the extent of decline in laboratory values was most likely dependent on volume of tissue treated.", "PMID": 971131}
{"id": "pubmed23n0032_8378", "title": "Prognostic criteria of sudden deafness as deduced by electrocochleography.", "content": "Electrocochleography was performed at first examination in 34 patients with sudden deafness. Recording was made with the use of the transtympanic needle electrode technique. A notable difference in the electrocochleographic findings was shown between the cases of complete recovery or remarkable improvement and the cases of slight improvement or no change. Electrocochleography may be prognostic of sudden deafness at initial onset.", "contents": "Prognostic criteria of sudden deafness as deduced by electrocochleography. Electrocochleography was performed at first examination in 34 patients with sudden deafness. Recording was made with the use of the transtympanic needle electrode technique. A notable difference in the electrocochleographic findings was shown between the cases of complete recovery or remarkable improvement and the cases of slight improvement or no change. Electrocochleography may be prognostic of sudden deafness at initial onset.", "PMID": 971132}
{"id": "pubmed23n0032_8379", "title": "Subjective and electrophysiologic tests in brain-stem lesions.", "content": "A battery of subjective and electrophysiologic auditory and vestibular tests were administered to 22 patients with neurologically confirmed brain-stem lesions to investigate the interrelationships between the test results. Acoustic-reflex and simultaneous midplane binaural loudness-balance abnormalities were related to N2 and N3 abnormalities on the brain-stem evoked-response measures. Unilateral abnormalities in low-redundancy speech tests, prolonged auditory reaction times, and reduced loudness growth on the alternate binaural loudness-balance test were related to ipsilateral N4 abnormalities or contralateral N5 abnormalities on the brain-stem evoked-response measures.", "contents": "Subjective and electrophysiologic tests in brain-stem lesions. A battery of subjective and electrophysiologic auditory and vestibular tests were administered to 22 patients with neurologically confirmed brain-stem lesions to investigate the interrelationships between the test results. Acoustic-reflex and simultaneous midplane binaural loudness-balance abnormalities were related to N2 and N3 abnormalities on the brain-stem evoked-response measures. Unilateral abnormalities in low-redundancy speech tests, prolonged auditory reaction times, and reduced loudness growth on the alternate binaural loudness-balance test were related to ipsilateral N4 abnormalities or contralateral N5 abnormalities on the brain-stem evoked-response measures.", "PMID": 971133}
{"id": "pubmed23n0032_8380", "title": "The cross-check principle in pediatric audiometry.", "content": "We discuss a method of pediatric audiologic assessment that employs the \"cross-check principle\". That is, the results of a single test are cross-checked by an independent test measure. Particularly useful in pediatric evaluations as cross-checks of behavioral test results are impedance audiometry and brain-stem-evoked response audiometry (BSER). We present five cases highlighting the value of the cross-check principle in pediatric audiologic evaluation.", "contents": "The cross-check principle in pediatric audiometry. We discuss a method of pediatric audiologic assessment that employs the \"cross-check principle\". That is, the results of a single test are cross-checked by an independent test measure. Particularly useful in pediatric evaluations as cross-checks of behavioral test results are impedance audiometry and brain-stem-evoked response audiometry (BSER). We present five cases highlighting the value of the cross-check principle in pediatric audiologic evaluation.", "PMID": 971134}
{"id": "pubmed23n0032_8381", "title": "Brief-tone audiometry in pseudohypacusis.", "content": "Brief-tone audiometry was administered to two groups of listeners. The first group consisted of five sophisticated subjects with normal hearing, who were tested under normal and simulated hearing-loss conditions. The second group consisted of five patients with true pseudohypacusis. Results under simulated and true pseudohypacusic conditions were similar, but were different from those obtained from the normal listeners under normal conditions, which further extends the diagnostic value of brief-tone audiometry.", "contents": "Brief-tone audiometry in pseudohypacusis. Brief-tone audiometry was administered to two groups of listeners. The first group consisted of five sophisticated subjects with normal hearing, who were tested under normal and simulated hearing-loss conditions. The second group consisted of five patients with true pseudohypacusis. Results under simulated and true pseudohypacusic conditions were similar, but were different from those obtained from the normal listeners under normal conditions, which further extends the diagnostic value of brief-tone audiometry.", "PMID": 971135}
{"id": "pubmed23n0032_8382", "title": "Mandibular fracture complications.", "content": "This paper presents the authors' experiences with 111 treated mandibular fractures and 16 cases of complication. Pertinent data on the fresh fractures are given, but the major part of the study deals with cause, diagnosis, and treatment of the complications of mandibular fractures. These are divided into four categories-infection, nonunion, malunion, and temporomandibular joint ankylosis, although only infection and temporomandibular joint ankylosis are included here. Unique mandibular fracture complications are reviewed.", "contents": "Mandibular fracture complications. This paper presents the authors' experiences with 111 treated mandibular fractures and 16 cases of complication. Pertinent data on the fresh fractures are given, but the major part of the study deals with cause, diagnosis, and treatment of the complications of mandibular fractures. These are divided into four categories-infection, nonunion, malunion, and temporomandibular joint ankylosis, although only infection and temporomandibular joint ankylosis are included here. Unique mandibular fracture complications are reviewed.", "PMID": 971136}
{"id": "pubmed23n0032_8383", "title": "Chromophobe adenoma manifesting as a nasopharyngeal mass.", "content": "A 64-year-old woman had a sphenoid sinus tumor and nasopharyngeal mass verified to be a nasopharyngeal chromophobe adenoma with no evidence of sellar enlargement or involvement. The possibility that this mass arose from ectopic hypophyseal tissue located in the pharynx is proposed.", "contents": "Chromophobe adenoma manifesting as a nasopharyngeal mass. A 64-year-old woman had a sphenoid sinus tumor and nasopharyngeal mass verified to be a nasopharyngeal chromophobe adenoma with no evidence of sellar enlargement or involvement. The possibility that this mass arose from ectopic hypophyseal tissue located in the pharynx is proposed.", "PMID": 971137}
{"id": "pubmed23n0032_8384", "title": "The effect of extensive chorioptic mange of the scrotum on reproductive function of the ram.", "content": "Reproductive function was assessed in 14 stud rams which had been rejected as genitally unsound because of extensive chorioptic mange (Chorioptes bovis) of the scrotum. Severe seminal degeneration was observed in all of these rams. Mean testicular weight of the affected rams was 72.2 +/- 18.4 (S.D.) g, while that of the non-affected control rams was 190.1 +/- 38.2g. The seminiferous tubules of the atrophic testes were severely shrunken and spermatogenic arrest occurred at the spermatogonia or primary spermatocyte stage. Androgenic status and libido as assessed by seminal fructose, seminal vesicle weight, seminal fructose and reaction time were not affected by the disease. The average testicular temperature of 11 rams with extensive scrotal mange and severe testicular atrophy was 0.6 degrees C to 3.1 degrees C (average 1.7 degrees C) higher than that recorded from 11 control rams. The exudative dermatitis did not penetrate the tunica vaginalis sac and had no apparent effect on the general health of the rams. It was concluded that scrotal mange causes testicular degeneration by raising the temperature of the scrotal contents. Four rams that had severe testicular atrophy with complete spermatogenic arrest showed an acceptable level of semen production following treatment of their mange lesions. Recovery of spermatozoal production was also observed following spontaneous cure of chorioptic mange lesions in a ram whose scrotum had become severely thickened and pendulous due to long-standing chrorioptic mange.", "contents": "The effect of extensive chorioptic mange of the scrotum on reproductive function of the ram. Reproductive function was assessed in 14 stud rams which had been rejected as genitally unsound because of extensive chorioptic mange (Chorioptes bovis) of the scrotum. Severe seminal degeneration was observed in all of these rams. Mean testicular weight of the affected rams was 72.2 +/- 18.4 (S.D.) g, while that of the non-affected control rams was 190.1 +/- 38.2g. The seminiferous tubules of the atrophic testes were severely shrunken and spermatogenic arrest occurred at the spermatogonia or primary spermatocyte stage. Androgenic status and libido as assessed by seminal fructose, seminal vesicle weight, seminal fructose and reaction time were not affected by the disease. The average testicular temperature of 11 rams with extensive scrotal mange and severe testicular atrophy was 0.6 degrees C to 3.1 degrees C (average 1.7 degrees C) higher than that recorded from 11 control rams. The exudative dermatitis did not penetrate the tunica vaginalis sac and had no apparent effect on the general health of the rams. It was concluded that scrotal mange causes testicular degeneration by raising the temperature of the scrotal contents. Four rams that had severe testicular atrophy with complete spermatogenic arrest showed an acceptable level of semen production following treatment of their mange lesions. Recovery of spermatozoal production was also observed following spontaneous cure of chorioptic mange lesions in a ram whose scrotum had become severely thickened and pendulous due to long-standing chrorioptic mange.", "PMID": 971142}
{"id": "pubmed23n0032_8385", "title": "The effect of Leptospira hardjo vaccine in a northern Queensland beef herd.", "content": "In a northern Queensland beef herd where there was serological evidence of L. hardjo infection, lactation failure or wastage between pregnancy diagnosis and branding was 11.9% in animals vaccinated with a single dose of a L. hardjo vaccine and 19.4% in unvaccinated controls. This difference was statistically significant (P less than 0.05). Vaccination caused a significant (P less than 0.05) reduction in rate of lactation failure in heifers but not in other classes of cows.", "contents": "The effect of Leptospira hardjo vaccine in a northern Queensland beef herd. In a northern Queensland beef herd where there was serological evidence of L. hardjo infection, lactation failure or wastage between pregnancy diagnosis and branding was 11.9% in animals vaccinated with a single dose of a L. hardjo vaccine and 19.4% in unvaccinated controls. This difference was statistically significant (P less than 0.05). Vaccination caused a significant (P less than 0.05) reduction in rate of lactation failure in heifers but not in other classes of cows.", "PMID": 971143}
{"id": "pubmed23n0032_8386", "title": "Helminth control in Queensland beef cattle: comparison of part paddock and whole paddock treatment in the wallum of south eastern Queensland.", "content": "An increase in the body weight gain of beef calves due to a reduction in the overall larval nematode challenge and a consequent reduction in the host response to infection was observed on fertilised, improved pasturesin the wallum country of south eastern Queensland. Three groups of calves were used. Group 1 consisted of 15 calves and their dams on 20 ha, all receiving monthly injectable tetramisole. Groups 2 and 3 consisted each of 15 calves and dams grazing 40 ha with only the 15 calves of Group 2 receiving monthly tetramisole. The trial concluded after 10 months when the mean body weight gains made by calves of Groups 1, 2 and 3 were 206 kg. 177 kg and 168 kg respectively. These differences proved statistically significant. All heifers from these groups were sold soon after the conclusion of the trial and sale prices of heifers from Group 1 were 11% higher than those from Group 2 and 21% higher than those from Group 3. During this work the predominat parasitic nematode present was cooperia spp H. placei and O. ostertagi were also prevalent species and O.E. radiatum was present in low numbers.", "contents": "Helminth control in Queensland beef cattle: comparison of part paddock and whole paddock treatment in the wallum of south eastern Queensland. An increase in the body weight gain of beef calves due to a reduction in the overall larval nematode challenge and a consequent reduction in the host response to infection was observed on fertilised, improved pasturesin the wallum country of south eastern Queensland. Three groups of calves were used. Group 1 consisted of 15 calves and their dams on 20 ha, all receiving monthly injectable tetramisole. Groups 2 and 3 consisted each of 15 calves and dams grazing 40 ha with only the 15 calves of Group 2 receiving monthly tetramisole. The trial concluded after 10 months when the mean body weight gains made by calves of Groups 1, 2 and 3 were 206 kg. 177 kg and 168 kg respectively. These differences proved statistically significant. All heifers from these groups were sold soon after the conclusion of the trial and sale prices of heifers from Group 1 were 11% higher than those from Group 2 and 21% higher than those from Group 3. During this work the predominat parasitic nematode present was cooperia spp H. placei and O. ostertagi were also prevalent species and O.E. radiatum was present in low numbers.", "PMID": 971144}
{"id": "pubmed23n0032_8387", "title": "Skeletal response of rats to repeated short-term hyperbaric helium-oxygen exposures.", "content": "Young male rats were exposed to repeated heliox dives and analyzed for skeletal alterations. Animals were exposed 1, 3, 5, or 7 times to either 1 ATA He-O2 for 12.5 h, or to 5 ATA He-O2 for 4 h and a 8.5 h decompression, or to 5 ATA He-O2 for 4 h and a 1.5 h decompression. In a separate study, 30 rats were exposed 6 times to 5 ATA He-O2 and explosively decompressed. Animals were sacrificed 20 d after the last dive. There were no significant changes in femur wet weight, density, ash weight, length, or mineral content. Plasma calcium, phosphorus, and alkaline phosphatase remained normal. Eighteen of 30 animals survived the six explosive decompressions; however, there were no significant changes in bone. These results indicate that the number and rate of decompressions used in this study have no lasting effect on bone growth and mineral composition in the rat.", "contents": "Skeletal response of rats to repeated short-term hyperbaric helium-oxygen exposures. Young male rats were exposed to repeated heliox dives and analyzed for skeletal alterations. Animals were exposed 1, 3, 5, or 7 times to either 1 ATA He-O2 for 12.5 h, or to 5 ATA He-O2 for 4 h and a 8.5 h decompression, or to 5 ATA He-O2 for 4 h and a 1.5 h decompression. In a separate study, 30 rats were exposed 6 times to 5 ATA He-O2 and explosively decompressed. Animals were sacrificed 20 d after the last dive. There were no significant changes in femur wet weight, density, ash weight, length, or mineral content. Plasma calcium, phosphorus, and alkaline phosphatase remained normal. Eighteen of 30 animals survived the six explosive decompressions; however, there were no significant changes in bone. These results indicate that the number and rate of decompressions used in this study have no lasting effect on bone growth and mineral composition in the rat.", "PMID": 971153}
{"id": "pubmed23n0032_8388", "title": "Influence of cold or hyperbaric helium-oxygen environments on mouse response to a respiratory viral infection.", "content": "In investigating the stress effects of chilling (2-3 degrees C) and hypothermia (2-3 degrees C drop in body core temperature mediated by exposure to hyperbaric helium-oxygen atmosphere) on mouse resistance to \"influenza,\" it was noted that these stresses adversely affected the course of pulmonary infection produced by aerosols of the NWS strain of influenza virus. Comparatively, respiratory LD50 values for control animals were about 25 virus plaque-forming units (PFU) with median mortality occurring on day 13. The LD50 values for mice chilled at 2-3 degrees C were about 15 PFU with median mortality on day 7, and for mice exposed to hyperbaric helium, about 12 PFU with median mortality on day 6. Cold or hyperbaric stress impaired interferon production. Impairment was observed at 24 h but not at 12 h post-challenge and persisted for several days until mice became moribund.", "contents": "Influence of cold or hyperbaric helium-oxygen environments on mouse response to a respiratory viral infection. In investigating the stress effects of chilling (2-3 degrees C) and hypothermia (2-3 degrees C drop in body core temperature mediated by exposure to hyperbaric helium-oxygen atmosphere) on mouse resistance to \"influenza,\" it was noted that these stresses adversely affected the course of pulmonary infection produced by aerosols of the NWS strain of influenza virus. Comparatively, respiratory LD50 values for control animals were about 25 virus plaque-forming units (PFU) with median mortality occurring on day 13. The LD50 values for mice chilled at 2-3 degrees C were about 15 PFU with median mortality on day 7, and for mice exposed to hyperbaric helium, about 12 PFU with median mortality on day 6. Cold or hyperbaric stress impaired interferon production. Impairment was observed at 24 h but not at 12 h post-challenge and persisted for several days until mice became moribund.", "PMID": 971154}
{"id": "pubmed23n0032_8389", "title": "Cytochemical investigations of proteins and RNA in spinal motoneurons and neurons of spinal ganglia of the rat after space flight.", "content": "The content of RNA and cytoplasmic protein was assayed by methods of quantitative cytochemistry in motoneurons of anterior horns of the spinal cord and neurons of spinal ganglia of rats flown for 22 d. It was shown that the 22-d space flight did not change the RNA content in motoneurons of anterior horns of the spinal cord and reduced the RNA content in large neurons of spinal ganglia. The protein content in the cytoplasm of motoneurons and neurons of spinal ganglia was significantly lowered as compared with the control. The RNA content 27 d postflight in motoneurons of anterior horns of the spinal cord was significantly reduced and in neurons of spinal ganglia it did not differ from the control. On the 27th postflight day the protein content in the cytoplasm of motoneurons returned to the preflight level and in the cytoplasm of large neurons of spinal ganglia remained lowered.", "contents": "Cytochemical investigations of proteins and RNA in spinal motoneurons and neurons of spinal ganglia of the rat after space flight. The content of RNA and cytoplasmic protein was assayed by methods of quantitative cytochemistry in motoneurons of anterior horns of the spinal cord and neurons of spinal ganglia of rats flown for 22 d. It was shown that the 22-d space flight did not change the RNA content in motoneurons of anterior horns of the spinal cord and reduced the RNA content in large neurons of spinal ganglia. The protein content in the cytoplasm of motoneurons and neurons of spinal ganglia was significantly lowered as compared with the control. The RNA content 27 d postflight in motoneurons of anterior horns of the spinal cord was significantly reduced and in neurons of spinal ganglia it did not differ from the control. On the 27th postflight day the protein content in the cytoplasm of motoneurons returned to the preflight level and in the cytoplasm of large neurons of spinal ganglia remained lowered.", "PMID": 971155}
{"id": "pubmed23n0032_8390", "title": "Cardiac pathology associated with high sustained +Gz: I. Subendocardial hemorrhage.", "content": "Adult miniature swine were exposed to various levels and durations of +Gz. After exposure, all swine were euthanized and necropsied. Gross, histologic, and electronmicroscopic observations were made on the heart tissue. Subendocaridal hemorrhage (SEH) was commonly found in the left ventricle, rarely in the right ventricle, and its severity was directly related to : a) level and duration of G exposure, b) heart rate, and c) catecholamine activity. SEH was made more severe with i.v. atropine 4 mg, and prevented with i.v. propranolol 20 mg. Heart hemorrhage was usually limited to the immediate subendocardial region and frequently surrounded Purkinje's fibers. In severe cases, however, hemorrhages penetrated several millimeters into the heart muscle and sometimes penetrated Purkinje's fibers. Restraint of unanesthetized swine in the centrifuge couch, low G-levels, and/or i.v. injections of atropine or epinephrine produced minimal SEH lesions.", "contents": "Cardiac pathology associated with high sustained +Gz: I. Subendocardial hemorrhage. Adult miniature swine were exposed to various levels and durations of +Gz. After exposure, all swine were euthanized and necropsied. Gross, histologic, and electronmicroscopic observations were made on the heart tissue. Subendocaridal hemorrhage (SEH) was commonly found in the left ventricle, rarely in the right ventricle, and its severity was directly related to : a) level and duration of G exposure, b) heart rate, and c) catecholamine activity. SEH was made more severe with i.v. atropine 4 mg, and prevented with i.v. propranolol 20 mg. Heart hemorrhage was usually limited to the immediate subendocardial region and frequently surrounded Purkinje's fibers. In severe cases, however, hemorrhages penetrated several millimeters into the heart muscle and sometimes penetrated Purkinje's fibers. Restraint of unanesthetized swine in the centrifuge couch, low G-levels, and/or i.v. injections of atropine or epinephrine produced minimal SEH lesions.", "PMID": 971156}
{"id": "pubmed23n0032_8391", "title": "Cardiac pathology associated with high sustained +Gz: II. Stress cardiomyopathy.", "content": "The myocardial pathology of 14 pigs exposed to HSGz stress of 9 and 15, or 3, 7, and 9 Gz was studied; six control pigs were used as comparisons. Four pigs received propranolol prior to centrifugation and four pigs received atropine. Hearts were studied by light and electron microscopy. Myocardium from stressed pigs showed myofibrillar degeneration, pooling of mitochondria, and cell death. Lesions occurred in random cells of the subendocardium and papillary muscles. Purkinje fibers were also involved. Pretreatment with atropine increased the number of dead cells found and propranolol increased the number of cells showing myofibrillar degeneration. It is postulated that this is a pluricausal cardiomyopathy similar to several experimental conditions. Significance to aerospace medicine is briefly discussed.", "contents": "Cardiac pathology associated with high sustained +Gz: II. Stress cardiomyopathy. The myocardial pathology of 14 pigs exposed to HSGz stress of 9 and 15, or 3, 7, and 9 Gz was studied; six control pigs were used as comparisons. Four pigs received propranolol prior to centrifugation and four pigs received atropine. Hearts were studied by light and electron microscopy. Myocardium from stressed pigs showed myofibrillar degeneration, pooling of mitochondria, and cell death. Lesions occurred in random cells of the subendocardium and papillary muscles. Purkinje fibers were also involved. Pretreatment with atropine increased the number of dead cells found and propranolol increased the number of cells showing myofibrillar degeneration. It is postulated that this is a pluricausal cardiomyopathy similar to several experimental conditions. Significance to aerospace medicine is briefly discussed.", "PMID": 971157}
{"id": "pubmed23n0032_8392", "title": "Changes in clinical cardiologic measurements associated with high +Gz stress.", "content": "Because of reports of subendocardial hemorrhage and myofibrillar degeneration in animals exposed to sustained high G loads (greater than +6 Gz for 15 s or more), questions have been raised regarding the safety of exposing pilots and human subjects to the similar G-stress levels likely to be encountered in the new high-performance fighter aircraft. Noninvasive clinical cardiologic data, including ECGs, vectorcardiograms, systolic time intervals, and maximal treadmill stress tests, were obtained from two groups of subjects before and at several times after exposure to high-G stress. The group exposed to the greater G stress (three 40-s runs at 8 G and two 40-s runs at 10 G, all in one day) developed moderate cutaneous petechiasis and had other minor physical findings after the G stress, but showed few significant changes in cardiologic data: serum total CPK and LDH levels rose, and preejection period shortened at 48 h poststress. The group exposed to the lesser G stress (one 100-s variable-G maneuver peaking twice at 8 G for 3 s, once a week for 3 weeks) had no symptoms following the G stress, but the vectorcardiograms revealed transient T-loop angle changes, and preejection period measured at 1 week poststress was significantly decreased. Because the serum enzyme changes were noncardiac in origin, and because the few other changes were not in a direction indicative of cardiac damage, we conclude that the G stresses imposed in these studies were not significantly injurious.", "contents": "Changes in clinical cardiologic measurements associated with high +Gz stress. Because of reports of subendocardial hemorrhage and myofibrillar degeneration in animals exposed to sustained high G loads (greater than +6 Gz for 15 s or more), questions have been raised regarding the safety of exposing pilots and human subjects to the similar G-stress levels likely to be encountered in the new high-performance fighter aircraft. Noninvasive clinical cardiologic data, including ECGs, vectorcardiograms, systolic time intervals, and maximal treadmill stress tests, were obtained from two groups of subjects before and at several times after exposure to high-G stress. The group exposed to the greater G stress (three 40-s runs at 8 G and two 40-s runs at 10 G, all in one day) developed moderate cutaneous petechiasis and had other minor physical findings after the G stress, but showed few significant changes in cardiologic data: serum total CPK and LDH levels rose, and preejection period shortened at 48 h poststress. The group exposed to the lesser G stress (one 100-s variable-G maneuver peaking twice at 8 G for 3 s, once a week for 3 weeks) had no symptoms following the G stress, but the vectorcardiograms revealed transient T-loop angle changes, and preejection period measured at 1 week poststress was significantly decreased. Because the serum enzyme changes were noncardiac in origin, and because the few other changes were not in a direction indicative of cardiac damage, we conclude that the G stresses imposed in these studies were not significantly injurious.", "PMID": 971158}
{"id": "pubmed23n0032_8393", "title": "Space flight effects on skeletal bones of rats (light and electron microscopic examination).", "content": "In the light and electron microscopes, long tubular bones of Wistar rats that were flown for 22 d onboard the Cosmos-605 biosatellite and were exposed to a ground-based simulation experiment were examined. About half of the flight rats showed osteoporosis of metaphyses which was usually combined with a decrease of the mass of the primary spongiosa in the vicinity of the epiphyseal cartilaginous plate. This gives evidence that the growth of the bones could have been inhibited in flight. The light and electron microscopy of bones of flight rats revealed wide osteocyte lacunae which could have been produced by perilacunar osteolysis. In the simulation experiment, reduction in the metaphyseal spongiosa occurred only in one-third of the rats and was less pronounced than in flight rats; no decrease of the mass of the primary spongiosa near the cartilaginous plate was noted. Histological investigation of bones 27 d postflight demonstrated that that time period was not enough to eliminate all the changes in the bones tested.", "contents": "Space flight effects on skeletal bones of rats (light and electron microscopic examination). In the light and electron microscopes, long tubular bones of Wistar rats that were flown for 22 d onboard the Cosmos-605 biosatellite and were exposed to a ground-based simulation experiment were examined. About half of the flight rats showed osteoporosis of metaphyses which was usually combined with a decrease of the mass of the primary spongiosa in the vicinity of the epiphyseal cartilaginous plate. This gives evidence that the growth of the bones could have been inhibited in flight. The light and electron microscopy of bones of flight rats revealed wide osteocyte lacunae which could have been produced by perilacunar osteolysis. In the simulation experiment, reduction in the metaphyseal spongiosa occurred only in one-third of the rats and was less pronounced than in flight rats; no decrease of the mass of the primary spongiosa near the cartilaginous plate was noted. Histological investigation of bones 27 d postflight demonstrated that that time period was not enough to eliminate all the changes in the bones tested.", "PMID": 971159}
{"id": "pubmed23n0032_8394", "title": "Effect of pre-adapting spectral stimuli on visual responses.", "content": "Electroretinograms (ERGs) were recorded following pre-adapting exposures to radiometrically and photometrically matched spectral stimuli. The magnitudes of three aspects of the ERG varied as a function of the pre-adapting wavelength. Longer wavelenght pre-adapting stimuli produced less suppression of the response magnitudes than stimuli from the short wavelenght end of the spectrum. The implications of the findings for display design and cockpit-light-source design and management are discussed.", "contents": "Effect of pre-adapting spectral stimuli on visual responses. Electroretinograms (ERGs) were recorded following pre-adapting exposures to radiometrically and photometrically matched spectral stimuli. The magnitudes of three aspects of the ERG varied as a function of the pre-adapting wavelength. Longer wavelenght pre-adapting stimuli produced less suppression of the response magnitudes than stimuli from the short wavelenght end of the spectrum. The implications of the findings for display design and cockpit-light-source design and management are discussed.", "PMID": 971160}
{"id": "pubmed23n0032_8395", "title": "Cardiovascular function during sustained +Gz stress.", "content": "The development of aerospace systems capable of very high levels of positive (+Gz) stress, has created a need for a better understanding of the cardiovascular responses to acceleration. Using a canine model, the heart and cardiovascular system were instrumented to continuously measure coronary blood flow, cardiac output, left ventricular and aortic root pressure, and oxygen saturation in the aorta, coronary sinus, and right ventricle. The animals were exposed to acceleration profiles up to +6 Gz, 120 s at peak G; a seatback angle of 45 degrees was simulated in some experiments. Radiopaque contrast medium was injected to visualize the left ventricular chamber, coronary vasculature, aorta, and branches of the aorta. The results suggest mechanisms responsible for arrhythmias which may occur, and subendocardial hemorrhage which has been reported in other animals.", "contents": "Cardiovascular function during sustained +Gz stress. The development of aerospace systems capable of very high levels of positive (+Gz) stress, has created a need for a better understanding of the cardiovascular responses to acceleration. Using a canine model, the heart and cardiovascular system were instrumented to continuously measure coronary blood flow, cardiac output, left ventricular and aortic root pressure, and oxygen saturation in the aorta, coronary sinus, and right ventricle. The animals were exposed to acceleration profiles up to +6 Gz, 120 s at peak G; a seatback angle of 45 degrees was simulated in some experiments. Radiopaque contrast medium was injected to visualize the left ventricular chamber, coronary vasculature, aorta, and branches of the aorta. The results suggest mechanisms responsible for arrhythmias which may occur, and subendocardial hemorrhage which has been reported in other animals.", "PMID": 971161}
{"id": "pubmed23n0032_8396", "title": "Application of hyperbaric oxygen therapy in a case of prolonged cerebral hypoxia following rapid decompression.", "content": "The first known and previously unpublished report of the use of hyperbaric oxygen (HBO) in the treatment of prolonged cerebral hypoxia is reported in an incident involving a USAF T-39 aircraft that underwent rapid decompression from 753 mb (2,438 m or 8,000 ft) to 148 mb (13,716 m or 45,000 ft). Within 5 to 8 s, the pilot lost consciousness because he did not don his oxygen mask and, therefore, was unable to obtain supplemental oxygen immediately. After a delay of 6 to 8 min, he was given supplemental oxygen at and below 697 mb (3,048 m or 10,000 ft). On the ground, the pilot was blind and disoriented. This condition persisted for 6.5 h, at which time HBO was administered. Orientation and vision were regained; neurologic findings were negative. It is concluded that HBO therapy was effective in treating this case of prolonged cerebral hypoxia. The report shows 1) the potential application of HBO in the treatment of prolonged cerebral hypoxia, and 2) the need for developing safe procedures for descent of passenger aircraft following such decompressions.", "contents": "Application of hyperbaric oxygen therapy in a case of prolonged cerebral hypoxia following rapid decompression. The first known and previously unpublished report of the use of hyperbaric oxygen (HBO) in the treatment of prolonged cerebral hypoxia is reported in an incident involving a USAF T-39 aircraft that underwent rapid decompression from 753 mb (2,438 m or 8,000 ft) to 148 mb (13,716 m or 45,000 ft). Within 5 to 8 s, the pilot lost consciousness because he did not don his oxygen mask and, therefore, was unable to obtain supplemental oxygen immediately. After a delay of 6 to 8 min, he was given supplemental oxygen at and below 697 mb (3,048 m or 10,000 ft). On the ground, the pilot was blind and disoriented. This condition persisted for 6.5 h, at which time HBO was administered. Orientation and vision were regained; neurologic findings were negative. It is concluded that HBO therapy was effective in treating this case of prolonged cerebral hypoxia. The report shows 1) the potential application of HBO in the treatment of prolonged cerebral hypoxia, and 2) the need for developing safe procedures for descent of passenger aircraft following such decompressions.", "PMID": 971162}
{"id": "pubmed23n0032_8397", "title": "Effects of time zone changes on performance and physiology of airline personnel.", "content": "This study is the second of a series of experiments describing the effects of time zone changes on the performance and menstrual cycle changes in a group of 16 female airline personnel. Four groups of four subjects each spent 4 d in the isolation unit of the University of Manchester. One group acted as control; the other three groups were subjected to two 8-h retardations in time, representing Westerly flight. All groups were required to complete an identical battery of workload tasks. In addition, the effects of a therapeutic preparation (mepiprazole hydochloride) were studied in a double-blind experiment. The results of the study confirmed previous experiments in that there was impaired performance in individuals subjected to time zone changes when work was carried out in local time, but the results were difficult to compare with those obtained in Easterly shifts. The therapeutic agent appeared to have little influence on the effect of time zone changes upon subjects, although they reported favourably on its use.", "contents": "Effects of time zone changes on performance and physiology of airline personnel. This study is the second of a series of experiments describing the effects of time zone changes on the performance and menstrual cycle changes in a group of 16 female airline personnel. Four groups of four subjects each spent 4 d in the isolation unit of the University of Manchester. One group acted as control; the other three groups were subjected to two 8-h retardations in time, representing Westerly flight. All groups were required to complete an identical battery of workload tasks. In addition, the effects of a therapeutic preparation (mepiprazole hydochloride) were studied in a double-blind experiment. The results of the study confirmed previous experiments in that there was impaired performance in individuals subjected to time zone changes when work was carried out in local time, but the results were difficult to compare with those obtained in Easterly shifts. The therapeutic agent appeared to have little influence on the effect of time zone changes upon subjects, although they reported favourably on its use.", "PMID": 971163}
{"id": "pubmed23n0032_8398", "title": "Psychotherapy and return to flying duties.", "content": "The medical records of a group of 112 successive cases refered for psychiatric consultation to the USAF School of Aerospace Medicine were reviewed. Fifty-eight (52%) of these were psychiatrically disqualified but felt to be amenable to psychotherapy, which was recommended. Followup contact was possible in 49 of these cases to show that 38 did, in fact, receive treatment. Of this later group, 18 were successfully returned to flying duties along with six more, who had no formal treatment beyond several consultative visits, for a total salvage rate of 49%. Psychotherapy had previously been proven to be effective under conditions of major wartime mobilization. Its use in peacetime and during limited warfare has been studied only sparsely, except in the German Air Force, and only for specific conditions in the Royal Air Force. Results of this study support the contention that psychotherapy is feasibly effective in allowing other wise disqualified fliers to return to flying duties.", "contents": "Psychotherapy and return to flying duties. The medical records of a group of 112 successive cases refered for psychiatric consultation to the USAF School of Aerospace Medicine were reviewed. Fifty-eight (52%) of these were psychiatrically disqualified but felt to be amenable to psychotherapy, which was recommended. Followup contact was possible in 49 of these cases to show that 38 did, in fact, receive treatment. Of this later group, 18 were successfully returned to flying duties along with six more, who had no formal treatment beyond several consultative visits, for a total salvage rate of 49%. Psychotherapy had previously been proven to be effective under conditions of major wartime mobilization. Its use in peacetime and during limited warfare has been studied only sparsely, except in the German Air Force, and only for specific conditions in the Royal Air Force. Results of this study support the contention that psychotherapy is feasibly effective in allowing other wise disqualified fliers to return to flying duties.", "PMID": 971164}
{"id": "pubmed23n0032_8399", "title": "Professional territoriality: a study of the expanded role of the nurse.", "content": "This study examines the expanded role of the nurse as a specific example of the extension of professional boundaries. Three characteristics of professional territoriality--autonomy, accountability, and identity--were selected as variables in the study. The specific objectives were to examine attitudes of members of the nursing and medical profession toward the expanded role of the nurse, and to test the usefulness of the concept of professional territoriality in understanding the expanded role of the nurse. Attitudes toward nurses functioning in an expanded role were examined for two aspects: beliefs as to what nurse practitioners are now doing and what they should be able to do. Data was obtained through questionnaries mailed to a random sample of nurses and physicians in Minnesota. As predicted, the researcher found that nurses held more positive attitudes toward nurse practitioners working in an expanded role than do physicians.", "contents": "Professional territoriality: a study of the expanded role of the nurse. This study examines the expanded role of the nurse as a specific example of the extension of professional boundaries. Three characteristics of professional territoriality--autonomy, accountability, and identity--were selected as variables in the study. The specific objectives were to examine attitudes of members of the nursing and medical profession toward the expanded role of the nurse, and to test the usefulness of the concept of professional territoriality in understanding the expanded role of the nurse. Attitudes toward nurses functioning in an expanded role were examined for two aspects: beliefs as to what nurse practitioners are now doing and what they should be able to do. Data was obtained through questionnaries mailed to a random sample of nurses and physicians in Minnesota. As predicted, the researcher found that nurses held more positive attitudes toward nurse practitioners working in an expanded role than do physicians.", "PMID": 971165}
{"id": "pubmed23n0032_8400", "title": "Resonance ultrasonic measurements of microscopic gas bubbles.", "content": "The positive identification and location of stationary or moving bubbles in human tissue is a persisting problem in the study and management of dysbarism. Since bubbles are resonant scatterers of ultrasound, while structures such as red-cell clumps and tissue interfaces are not, it is possible in principle to identify bubbles uniquely and to determine their position and size. A pulsed, ultrasonic echo-ranging system was expressly designed to exploit the principle of bubble resonance, and was evaluated for bubble identification and location in two experiments. Echos from bubbles in a water bath were computer-processed to reveal a distinct \"line-narrowing\" in the Fourier domain, which is diagnostic of gas bubbles. An increase in echo signal from a dog's jugular vein upon distal bubble injecton was obtained as evidence for in vivo bubble detection. We conclude that the exploitation of the bubble resonance principle can offer a safe, noninvasive technology for micro-bubble identification, location, and size determination in many tissues. This technology appears capable of measuring stationary bubbles in tissue spaces or impacted in blood vessels as well as those moving in the vascular system.", "contents": "Resonance ultrasonic measurements of microscopic gas bubbles. The positive identification and location of stationary or moving bubbles in human tissue is a persisting problem in the study and management of dysbarism. Since bubbles are resonant scatterers of ultrasound, while structures such as red-cell clumps and tissue interfaces are not, it is possible in principle to identify bubbles uniquely and to determine their position and size. A pulsed, ultrasonic echo-ranging system was expressly designed to exploit the principle of bubble resonance, and was evaluated for bubble identification and location in two experiments. Echos from bubbles in a water bath were computer-processed to reveal a distinct \"line-narrowing\" in the Fourier domain, which is diagnostic of gas bubbles. An increase in echo signal from a dog's jugular vein upon distal bubble injecton was obtained as evidence for in vivo bubble detection. We conclude that the exploitation of the bubble resonance principle can offer a safe, noninvasive technology for micro-bubble identification, location, and size determination in many tissues. This technology appears capable of measuring stationary bubbles in tissue spaces or impacted in blood vessels as well as those moving in the vascular system.", "PMID": 971166}
{"id": "pubmed23n0032_8401", "title": "Visual elements in flight simulation.", "content": "Flight simulators have been in use for many years and their value has been amply proven. Many of today's simulators are very expensive, but they can pay for themselves by decreasing cost and increasing safety in training pilots to fly complex and expensive modern aircraft. Some aspects of aircraft control depend on an exterior view from the aircraft to the outside visual world. Training in these aspects of flight in a simulator requires simulation of the visual world to the extent that cues derived from it need to be employed by the pilot. A number of maneuvers cannot be performed without direct visual contact under normal circumstances in commercial as well as in military aviation. The importance of including a simulation of the external world is not acknowledged. Unfortunately, there is currently no solid scientific basis for cataloging visual cues with respect to their importance in aircraft control. This report recommends research topics, techniques, and strategies that should receive more attention.", "contents": "Visual elements in flight simulation. Flight simulators have been in use for many years and their value has been amply proven. Many of today's simulators are very expensive, but they can pay for themselves by decreasing cost and increasing safety in training pilots to fly complex and expensive modern aircraft. Some aspects of aircraft control depend on an exterior view from the aircraft to the outside visual world. Training in these aspects of flight in a simulator requires simulation of the visual world to the extent that cues derived from it need to be employed by the pilot. A number of maneuvers cannot be performed without direct visual contact under normal circumstances in commercial as well as in military aviation. The importance of including a simulation of the external world is not acknowledged. Unfortunately, there is currently no solid scientific basis for cataloging visual cues with respect to their importance in aircraft control. This report recommends research topics, techniques, and strategies that should receive more attention.", "PMID": 971167}
{"id": "pubmed23n0032_8402", "title": "Stress in air traffic controllers: effects of ARTS-III.", "content": "Physiological, biochemical, and psychological assessments of stress in air traffic controllers were made at Los Angeles (LAX) and Oakland (OAK) Terminal Radar Approach Control (TRACON) facilities before and after installation of Automated Radar Terminal Systems-III (ARTS-III). Heart rates of controllers on duty or at rest scarcely changed from before to after ARTS-III installation. Total stress increased at both TRACONs, and the increase was entirely due to elevated catecholamine excretion. Steroid excretion was significantly reduced at both facilities after ARTS-III had no appreciable effect on work-related anxiety levels of controllers. The post-ARTS-III A-State means for both facilities were not significantly elevated. A-Trait was unchanged at LAX but decreased significantly at OAK. Assessments of A-State showed significant work-related increments but tended to be low. There were no correlations between anxiety and physiological data.", "contents": "Stress in air traffic controllers: effects of ARTS-III. Physiological, biochemical, and psychological assessments of stress in air traffic controllers were made at Los Angeles (LAX) and Oakland (OAK) Terminal Radar Approach Control (TRACON) facilities before and after installation of Automated Radar Terminal Systems-III (ARTS-III). Heart rates of controllers on duty or at rest scarcely changed from before to after ARTS-III installation. Total stress increased at both TRACONs, and the increase was entirely due to elevated catecholamine excretion. Steroid excretion was significantly reduced at both facilities after ARTS-III had no appreciable effect on work-related anxiety levels of controllers. The post-ARTS-III A-State means for both facilities were not significantly elevated. A-Trait was unchanged at LAX but decreased significantly at OAK. Assessments of A-State showed significant work-related increments but tended to be low. There were no correlations between anxiety and physiological data.", "PMID": 971168}
{"id": "pubmed23n0032_8403", "title": "Nystagmus, turning sensations, and illusory movement in motion sickness susceptibility.", "content": "The relationships of motion sickness susceptibility to nystagmic eye movements, sensations of turning, and duration of the spiral aftereffect were determined with 48 subjects separated into groups on the basis of sex and extremes of motion sickness susceptibility. When subjective alertness levels were controlled, there was no enhancement of elicited nystagmus nor turning sensations in susceptible individuals. However, spiral aftereffect durations were significantly longer in susceptible individuals than in nonsusceptible individuals. The results are interpreted as favoring Graybiel's model of the structural elements in motion sickness.", "contents": "Nystagmus, turning sensations, and illusory movement in motion sickness susceptibility. The relationships of motion sickness susceptibility to nystagmic eye movements, sensations of turning, and duration of the spiral aftereffect were determined with 48 subjects separated into groups on the basis of sex and extremes of motion sickness susceptibility. When subjective alertness levels were controlled, there was no enhancement of elicited nystagmus nor turning sensations in susceptible individuals. However, spiral aftereffect durations were significantly longer in susceptible individuals than in nonsusceptible individuals. The results are interpreted as favoring Graybiel's model of the structural elements in motion sickness.", "PMID": 971169}
{"id": "pubmed23n0032_8404", "title": "Effect of sequential anti-G suit inflation on pulmonary capillary blood flow in man.", "content": "The hemodynamic effects of an anti-G suit, sequentially filled from below upward (M-aG), were compared to those of the standard USAF anti-G suit (S-aG) in 10 men supine and under +1 Gz stress produced by 90 degrees head up passive tilt. S-aG was found to fill from the abdominal bladder downward. The heart rate and the pulmonary capillary blood flow (Qc), as estimated by a nitrous oxide plethysmographic method, were used as criteria of effectiveness. Heart rate did not vary between the suits during the supine and upright studies. In the supine position, the first M-aG inflation induced a significant increase of Qc, 2.5 1 min above the Qc of the S-aG inflation. After 90 degrees head up tilt, M-aG inflation as associated with a significant increase of Qc, 1.7 1 min greater than the Qc produced by S-aG inflation. Since leg bladder inflation may play a critical role in high-G tolerance, the sequential filling from below upward of the anti-G suit may further increase the G protection compared to the standard garment. In addition, the modified anti-G suit may be a useful device for treatment of circulatory shock and postural hypotension when the venous return is decreased.", "contents": "Effect of sequential anti-G suit inflation on pulmonary capillary blood flow in man. The hemodynamic effects of an anti-G suit, sequentially filled from below upward (M-aG), were compared to those of the standard USAF anti-G suit (S-aG) in 10 men supine and under +1 Gz stress produced by 90 degrees head up passive tilt. S-aG was found to fill from the abdominal bladder downward. The heart rate and the pulmonary capillary blood flow (Qc), as estimated by a nitrous oxide plethysmographic method, were used as criteria of effectiveness. Heart rate did not vary between the suits during the supine and upright studies. In the supine position, the first M-aG inflation induced a significant increase of Qc, 2.5 1 min above the Qc of the S-aG inflation. After 90 degrees head up tilt, M-aG inflation as associated with a significant increase of Qc, 1.7 1 min greater than the Qc produced by S-aG inflation. Since leg bladder inflation may play a critical role in high-G tolerance, the sequential filling from below upward of the anti-G suit may further increase the G protection compared to the standard garment. In addition, the modified anti-G suit may be a useful device for treatment of circulatory shock and postural hypotension when the venous return is decreased.", "PMID": 971170}
{"id": "pubmed23n0032_8405", "title": "Protection of airline flight attendants from hypoxia following rapid decompression.", "content": "To determine the maximum time for working flight attendants to effectively initiate airline passenger mask donning after onset of a rapid, severe decompression, we exposed 10 subjects in two series of tests to a decompression from 6,500 to 34,000 ft (2,000 to 10,400 m) in 26 s, followed by descent at 5,000 ft/min (1,500 m/min) while subjects performed a light-to-moderate workload. Supplemental oxygen was provided in one series from a compressed oxygen system, and in the other series from a chemical oxygen generator system. With delays to mask doning of 10 and 15 s, no hypoxic effects occurred. With delays of 20 and 25 s, increasing hypoxic effects, similar in frequency for the two systems, occurred. Some technical problems in mask donning contributed to losses of consciousness with the latter two delays.", "contents": "Protection of airline flight attendants from hypoxia following rapid decompression. To determine the maximum time for working flight attendants to effectively initiate airline passenger mask donning after onset of a rapid, severe decompression, we exposed 10 subjects in two series of tests to a decompression from 6,500 to 34,000 ft (2,000 to 10,400 m) in 26 s, followed by descent at 5,000 ft/min (1,500 m/min) while subjects performed a light-to-moderate workload. Supplemental oxygen was provided in one series from a compressed oxygen system, and in the other series from a chemical oxygen generator system. With delays to mask doning of 10 and 15 s, no hypoxic effects occurred. With delays of 20 and 25 s, increasing hypoxic effects, similar in frequency for the two systems, occurred. Some technical problems in mask donning contributed to losses of consciousness with the latter two delays.", "PMID": 971171}
{"id": "pubmed23n0032_8406", "title": "Changes in blood enzyme activity and hematology of rats with decompression sickness.", "content": "Plasma activities of lactic dehydrogenase (LDH), creatine phosphokinase (CPK), glutamic pyruvic transaminase (GPT), and alkaline phosphatase (ALP) were studied, along with hematological changes, in rats suffering from various degrees of experimental decompression sickness (DS). By 1 h after decompression, LDH and CPK activities were elevated in moderate and severe DS whereas GOT and GPT were elevated only in severe DS. ALP was reduced in all decompressed rats. Hematological changes indicated hemoconcentration, the degree of which paralleled the severity of DS. By 24 h after decompression, all enzyme activities were approaching control levels with the exception of GOT, which was further elevated from the 1-h value. The observed lung damage in rats with severe DS in conjunction with the hematologic and enzyme data suggested that hypoxemic-hypoxia, incident to bubble embolization of pulmonary vasculature, was a major factor in altering the blood enzyme pattern in DS. Serum enzyme data from two inadequately decompressed divers are also reported.", "contents": "Changes in blood enzyme activity and hematology of rats with decompression sickness. Plasma activities of lactic dehydrogenase (LDH), creatine phosphokinase (CPK), glutamic pyruvic transaminase (GPT), and alkaline phosphatase (ALP) were studied, along with hematological changes, in rats suffering from various degrees of experimental decompression sickness (DS). By 1 h after decompression, LDH and CPK activities were elevated in moderate and severe DS whereas GOT and GPT were elevated only in severe DS. ALP was reduced in all decompressed rats. Hematological changes indicated hemoconcentration, the degree of which paralleled the severity of DS. By 24 h after decompression, all enzyme activities were approaching control levels with the exception of GOT, which was further elevated from the 1-h value. The observed lung damage in rats with severe DS in conjunction with the hematologic and enzyme data suggested that hypoxemic-hypoxia, incident to bubble embolization of pulmonary vasculature, was a major factor in altering the blood enzyme pattern in DS. Serum enzyme data from two inadequately decompressed divers are also reported.", "PMID": 971172}
{"id": "pubmed23n0032_8407", "title": "Identification of an apprehension effect on physiological indices of thermal strain.", "content": "A laboratory assessment of the thermal strain produced by wearing a protective clothing assembly, in addition to a normal flying clothing assembly, in a hot environment is described. In addition to the demonstration of an increase in thermal strain when wearing the protective clothing, two points of importance emerged. First, condition replication revealed a significant apprehension effect which confounded the physiological variables used to evaluate the thermal strain, leading to an over-estimation of the severity of that strain. Second, there was a large between-subject variation amongst the eight subjects used, which made the arithmetic mean a potentially misleading statistic for evaluating the increased physiological strain caused by the additional clothing assembly during the heat exposure. The practical implications of these observations are discussed....", "contents": "Identification of an apprehension effect on physiological indices of thermal strain. A laboratory assessment of the thermal strain produced by wearing a protective clothing assembly, in addition to a normal flying clothing assembly, in a hot environment is described. In addition to the demonstration of an increase in thermal strain when wearing the protective clothing, two points of importance emerged. First, condition replication revealed a significant apprehension effect which confounded the physiological variables used to evaluate the thermal strain, leading to an over-estimation of the severity of that strain. Second, there was a large between-subject variation amongst the eight subjects used, which made the arithmetic mean a potentially misleading statistic for evaluating the increased physiological strain caused by the additional clothing assembly during the heat exposure. The practical implications of these observations are discussed....", "PMID": 971173}
{"id": "pubmed23n0032_8408", "title": "Selective toxicity of 1 atmosphere of oxygen during morphogenesis of two Lepidopterans.", "content": "Continuous and interval exposures to 1 atmosphere of oxygen (hyperoxia) were examined using insects. Hyperoxia did not affect hatchability of Heliothis zea or Trichoplusia ni. Continuous hyperoxia was 100% lethal for H. zeal and T. ni. Most insects died as larvae and pupae of H. zea which resulted were deformed, reduced in weight, and failed to emerge. Hyperoxic exposures of T. ni for 48 h at sequential 48-h intervals during development, revealed that first instar and prepupae were most sensitive to hyperoxia and 80% were killed when exposed to only 24 h of hyperoxia as prepupae. T. ni which survived hyperoxia exposures at all development stages tested, were capable of producing progeny. The differential hyperoxic sensitivity and its correlation with specific morphogenetic stages suggest the usefulness of these insect species for studying biochemical sites of oxygen toxicity.", "contents": "Selective toxicity of 1 atmosphere of oxygen during morphogenesis of two Lepidopterans. Continuous and interval exposures to 1 atmosphere of oxygen (hyperoxia) were examined using insects. Hyperoxia did not affect hatchability of Heliothis zea or Trichoplusia ni. Continuous hyperoxia was 100% lethal for H. zeal and T. ni. Most insects died as larvae and pupae of H. zea which resulted were deformed, reduced in weight, and failed to emerge. Hyperoxic exposures of T. ni for 48 h at sequential 48-h intervals during development, revealed that first instar and prepupae were most sensitive to hyperoxia and 80% were killed when exposed to only 24 h of hyperoxia as prepupae. T. ni which survived hyperoxia exposures at all development stages tested, were capable of producing progeny. The differential hyperoxic sensitivity and its correlation with specific morphogenetic stages suggest the usefulness of these insect species for studying biochemical sites of oxygen toxicity.", "PMID": 971174}
{"id": "pubmed23n0032_8409", "title": "Mathematical model of man's tolerance to cold using morphological factors.", "content": "A mathematical model has been developed to anticipate the physiological responses and the thermal state of a naked human under exposure to cold, taking into account his morphological characteristics (skinfold, size, weight) and the environmental conditions (air or water temperature and velocity, barometric pressure and hygrometry). The skinfold conditions the body's thermal conductance and the metabolism depends both on rectal (Tre) and mean skin (Tsk) temperatures. After being tested, this model was used to study the evolution of Tre. It shows the influence of the skinfold which accounts for most of the inter-individual differences. It also permits discussion of survival possibilities during immersion and completes data provided by previously established curves.", "contents": "Mathematical model of man's tolerance to cold using morphological factors. A mathematical model has been developed to anticipate the physiological responses and the thermal state of a naked human under exposure to cold, taking into account his morphological characteristics (skinfold, size, weight) and the environmental conditions (air or water temperature and velocity, barometric pressure and hygrometry). The skinfold conditions the body's thermal conductance and the metabolism depends both on rectal (Tre) and mean skin (Tsk) temperatures. After being tested, this model was used to study the evolution of Tre. It shows the influence of the skinfold which accounts for most of the inter-individual differences. It also permits discussion of survival possibilities during immersion and completes data provided by previously established curves.", "PMID": 971175}
{"id": "pubmed23n0032_8410", "title": "Interaction of hyperbaric nitrogen and oxygen effects on behavior.", "content": "Breathing elevated environmental pressures of nitrogen and oxygen produced changes in behavior of rats that evidenced an interaction between the two gases. Rates of responding generated by a fixed-interval schedule of reinforcement systematically changed as partial pressures of nitrogen were elevated from 0.8 to 10.4 ATA. Response rates increased above baseline followed by a decline in rates as a function of increasing nitrogen pressure. Concurrent increases in partial pressures of oxygen from 0.2 to 2.2 ATA potentiated the rate-increasing effects of nitrogen at low to moderate nitrogen pressures and enhanced rate-decreasing effects at higher nitrogen pressures. Raised oxygen pressures modulated and interacted with the narcotic effects of nitrogen on behavior.", "contents": "Interaction of hyperbaric nitrogen and oxygen effects on behavior. Breathing elevated environmental pressures of nitrogen and oxygen produced changes in behavior of rats that evidenced an interaction between the two gases. Rates of responding generated by a fixed-interval schedule of reinforcement systematically changed as partial pressures of nitrogen were elevated from 0.8 to 10.4 ATA. Response rates increased above baseline followed by a decline in rates as a function of increasing nitrogen pressure. Concurrent increases in partial pressures of oxygen from 0.2 to 2.2 ATA potentiated the rate-increasing effects of nitrogen at low to moderate nitrogen pressures and enhanced rate-decreasing effects at higher nitrogen pressures. Raised oxygen pressures modulated and interacted with the narcotic effects of nitrogen on behavior.", "PMID": 971176}
{"id": "pubmed23n0032_8411", "title": "Physiological effects of solar heat load in a fighter cockpit.", "content": "The use of bubble canopies to improve vision in fighter aircraft exposes the cockpit to a high radiant heat load. Incoming sunlight increases the heat stress on crewmembers, both by raising air temperature and by directly heating exposed skin and clothing. An F-15 aircraft at Edwards AFB was modified to permit cockpit ventilation by external ground carts. Eight volunteers from the Test Pilot School were studied during 1-h periods in the closed cockpit, in sun and in shade. Mean cockpit air temperatures were 35.2 degrees C in shade and 51.9 degrees C in sun with PH2O less than 10 torr. The corresponding WBGT's were 22.6 and 36.4 degrees C. Sunlight added significantly to overall heat stress, as indicated by a rising heart rate and evaporative weight loss of 284 g/m2 - h (shade value was 109 g/m2 - hr). Mean skin temperatures were 34.3 degrees C in shade and 35.8 degrees C in sun. Particularly high skin temperatures were observed on the chest, the forehead and the top of the head under the helmet. The legs remained cool due to the flow of conditioned air, and this may explain why rectal temperature showed no meaningful change. Heat stress, which alone poses no physiological hazard, may cause crew performance decrements as well as diminishing acceleration tolerance. Possible means of eliminating or ameliorating these effects are discussed.", "contents": "Physiological effects of solar heat load in a fighter cockpit. The use of bubble canopies to improve vision in fighter aircraft exposes the cockpit to a high radiant heat load. Incoming sunlight increases the heat stress on crewmembers, both by raising air temperature and by directly heating exposed skin and clothing. An F-15 aircraft at Edwards AFB was modified to permit cockpit ventilation by external ground carts. Eight volunteers from the Test Pilot School were studied during 1-h periods in the closed cockpit, in sun and in shade. Mean cockpit air temperatures were 35.2 degrees C in shade and 51.9 degrees C in sun with PH2O less than 10 torr. The corresponding WBGT's were 22.6 and 36.4 degrees C. Sunlight added significantly to overall heat stress, as indicated by a rising heart rate and evaporative weight loss of 284 g/m2 - h (shade value was 109 g/m2 - hr). Mean skin temperatures were 34.3 degrees C in shade and 35.8 degrees C in sun. Particularly high skin temperatures were observed on the chest, the forehead and the top of the head under the helmet. The legs remained cool due to the flow of conditioned air, and this may explain why rectal temperature showed no meaningful change. Heat stress, which alone poses no physiological hazard, may cause crew performance decrements as well as diminishing acceleration tolerance. Possible means of eliminating or ameliorating these effects are discussed.", "PMID": 971177}
{"id": "pubmed23n0032_8412", "title": "Intravascular bubbles associated with intravenous injections and altitude.", "content": "Ultrasonically detected microbubbles were more abundant in the pulmonary artery of dogs intravenously injected with 10 ml of saline than in the same noninjected controls during 10,000 ft (3,048 m), 20,000 ft (6,096 m), and 40,000 ft (12,192 m) exposures. Continuous intravenous (i.v.) drip infusions also introduced many small bubbles. Since they may serve as \"nuclei\" for visible intravascular bubble formation, are sometimes associated with decompression sickness, and are additionally considered undesirable, it would appear prudent to minimize i.v. injections immediately before flights. However, a 10-min delay before ascent will reduce their number and a 60-min delay will insure their almost complete absence. Also, slow ascent, a 1-h denitrogenation time, or use of a degassed solution will help reduce their total number.", "contents": "Intravascular bubbles associated with intravenous injections and altitude. Ultrasonically detected microbubbles were more abundant in the pulmonary artery of dogs intravenously injected with 10 ml of saline than in the same noninjected controls during 10,000 ft (3,048 m), 20,000 ft (6,096 m), and 40,000 ft (12,192 m) exposures. Continuous intravenous (i.v.) drip infusions also introduced many small bubbles. Since they may serve as \"nuclei\" for visible intravascular bubble formation, are sometimes associated with decompression sickness, and are additionally considered undesirable, it would appear prudent to minimize i.v. injections immediately before flights. However, a 10-min delay before ascent will reduce their number and a 60-min delay will insure their almost complete absence. Also, slow ascent, a 1-h denitrogenation time, or use of a degassed solution will help reduce their total number.", "PMID": 971178}
{"id": "pubmed23n0032_8413", "title": "Psychomotor test performance and sleep patterns of aircrew flying transmeridional routes.", "content": "Pilots and flight attendants flying scheduled services between Vancouver and Tokyo and between Toronto and Rome were tested on a tracking task before and after flights in each direction. Flights were included in schedules involving both 24-h and 7-d layovers at the overseas station. During these periods, they recorded their sleep patterns. The data showed that, following flight, subjects made an immediate attempt to adapt their behaviour to local time and the changes in their performance scores could be interpreted on that basis. It was concluded that behavioural circadian rhythms adapt rapidly to a new time zone.", "contents": "Psychomotor test performance and sleep patterns of aircrew flying transmeridional routes. Pilots and flight attendants flying scheduled services between Vancouver and Tokyo and between Toronto and Rome were tested on a tracking task before and after flights in each direction. Flights were included in schedules involving both 24-h and 7-d layovers at the overseas station. During these periods, they recorded their sleep patterns. The data showed that, following flight, subjects made an immediate attempt to adapt their behaviour to local time and the changes in their performance scores could be interpreted on that basis. It was concluded that behavioural circadian rhythms adapt rapidly to a new time zone.", "PMID": 971179}
{"id": "pubmed23n0032_8414", "title": "Long-duration exposure to intermittent noises.", "content": "The effects were examined of various patterns of interruption--of continuous noise by periods of quiet--on the growth and recovery of temporary threshold shift of hearing over an exposure period of 24 h. Monaural thresholds of hearing were measured prior to, during, and following exposure to a pink noise at a level of 85 dBA and to four conditions in which the pink noise was interrupted with various on-off ratios. The interrupted exposure patterns and levels were adjusted to make their average levels equivalent to 85 dBA. Among the results: 1) the growth of TTS clear.ly reached an asymptote for all interrupted exposure conditions, even when the TTS was as small as 5 dB, 2) the interrupted exposures produced lower asymptotic levels than the continuous exposure with the same amount of energy, and 3) the TTS recovery patterns were essentially the same at 1 h and beyond, for all conditions. These data support the concept that, following a long exposure, one should be provided at least as much time for recovery as the duration of the exposure.", "contents": "Long-duration exposure to intermittent noises. The effects were examined of various patterns of interruption--of continuous noise by periods of quiet--on the growth and recovery of temporary threshold shift of hearing over an exposure period of 24 h. Monaural thresholds of hearing were measured prior to, during, and following exposure to a pink noise at a level of 85 dBA and to four conditions in which the pink noise was interrupted with various on-off ratios. The interrupted exposure patterns and levels were adjusted to make their average levels equivalent to 85 dBA. Among the results: 1) the growth of TTS clear.ly reached an asymptote for all interrupted exposure conditions, even when the TTS was as small as 5 dB, 2) the interrupted exposures produced lower asymptotic levels than the continuous exposure with the same amount of energy, and 3) the TTS recovery patterns were essentially the same at 1 h and beyond, for all conditions. These data support the concept that, following a long exposure, one should be provided at least as much time for recovery as the duration of the exposure.", "PMID": 971180}
{"id": "pubmed23n0032_8415", "title": "Perception of everyday visual environments during saccadic eye movements.", "content": "Subjects were required to execute saccadic eye movements in the horizontal plane which passed through primary gaze. During the saccades, visual images were projected onto a screen which subtended 40 degrees horizontaloy and 26 degrees vertically and was centered on primary gaze. Content, contrast, and intensity of the stimulus patterns and level of illumination of the laboratory background were manipulated to maximise pattern recognition. Little or no detail of the projected images could be discerned under any conditions. Only horizontal laminations were perceived as blurs of appropriate colour. It is concluded that there is no useful perception of the everyday environment during saccades.", "contents": "Perception of everyday visual environments during saccadic eye movements. Subjects were required to execute saccadic eye movements in the horizontal plane which passed through primary gaze. During the saccades, visual images were projected onto a screen which subtended 40 degrees horizontaloy and 26 degrees vertically and was centered on primary gaze. Content, contrast, and intensity of the stimulus patterns and level of illumination of the laboratory background were manipulated to maximise pattern recognition. Little or no detail of the projected images could be discerned under any conditions. Only horizontal laminations were perceived as blurs of appropriate colour. It is concluded that there is no useful perception of the everyday environment during saccades.", "PMID": 971181}
{"id": "pubmed23n0032_8416", "title": "Screening test for decompression sickness.", "content": "The fibrin-fibrinogen degradation products (FDP) tests were studied in 18 patients having a history of illness associated with diving. FDP tests were performed prior to hyperbaric oxygen therapy (OHP). Eight patients were serious neurologic signs had positive FDP tests and required repetitive therapy. Six patients had negative FDP tests with local musculoskeletal complaints and all were asymptomatic following the first OHP treatment. Three patients were found to be suffering from other diseases. These three patients had normal levels of FDP. One patient treated at another facility 3 months earlier and having paraplegia had a positive FDP test. Serious decompression sickness with neurologic complaints appear to have some degree of disseminated intravascular coagulation (DIC) as reflected by the FDP tests. The FDP test appears to be a useful screening test that may be able to delineate therapy.", "contents": "Screening test for decompression sickness. The fibrin-fibrinogen degradation products (FDP) tests were studied in 18 patients having a history of illness associated with diving. FDP tests were performed prior to hyperbaric oxygen therapy (OHP). Eight patients were serious neurologic signs had positive FDP tests and required repetitive therapy. Six patients had negative FDP tests with local musculoskeletal complaints and all were asymptomatic following the first OHP treatment. Three patients were found to be suffering from other diseases. These three patients had normal levels of FDP. One patient treated at another facility 3 months earlier and having paraplegia had a positive FDP test. Serious decompression sickness with neurologic complaints appear to have some degree of disseminated intravascular coagulation (DIC) as reflected by the FDP tests. The FDP test appears to be a useful screening test that may be able to delineate therapy.", "PMID": 971182}
{"id": "pubmed23n0032_8417", "title": "Incidence of decompression sickness in Navy low-pressure chambers.", "content": "This study reports the incidence of decompression sickness occurring in U. S. Navy altitude chambers in association with physiological training of aircrews for the period 1 Jan. 1972 to 31 Dec. 1975. There were 79 cases of decompression sickness in 88,520 altitude chamber exposures, an incidence of 0.089%. Among trainees, there were 22 cases in 73,561 exposures, an incidence of 0.029%. Among chamber inside observers, there were 57 cases in 14,959 exposures, an incidence of 0.38%. This 12-fold greater incidence among inside observers over trainees was statistically significant (p less than 0.01). Reasons for the increased incidence of decompression sickness among inside observers are discussed.", "contents": "Incidence of decompression sickness in Navy low-pressure chambers. This study reports the incidence of decompression sickness occurring in U. S. Navy altitude chambers in association with physiological training of aircrews for the period 1 Jan. 1972 to 31 Dec. 1975. There were 79 cases of decompression sickness in 88,520 altitude chamber exposures, an incidence of 0.089%. Among trainees, there were 22 cases in 73,561 exposures, an incidence of 0.029%. Among chamber inside observers, there were 57 cases in 14,959 exposures, an incidence of 0.38%. This 12-fold greater incidence among inside observers over trainees was statistically significant (p less than 0.01). Reasons for the increased incidence of decompression sickness among inside observers are discussed.", "PMID": 971183}
{"id": "pubmed23n0032_8418", "title": "Characteristics of ultrasonic scattered signals from emboli in blood.", "content": "Received ultrasonic scattered signal expressions are presented for the case of an individual scatterer moving through a Gaussian beam pattern. The return signal is of a burst-like nature and oscillates as a function of time under a Gaussian amplitude envelope. Results are presented to show specific parameter dependence on the scatterer motion and beam configuration to aid in the use of the technique of ultrasonic scattering for detecting and monitoring the motion of emboli formed in blood during decompression or open-heart surgery.", "contents": "Characteristics of ultrasonic scattered signals from emboli in blood. Received ultrasonic scattered signal expressions are presented for the case of an individual scatterer moving through a Gaussian beam pattern. The return signal is of a burst-like nature and oscillates as a function of time under a Gaussian amplitude envelope. Results are presented to show specific parameter dependence on the scatterer motion and beam configuration to aid in the use of the technique of ultrasonic scattering for detecting and monitoring the motion of emboli formed in blood during decompression or open-heart surgery.", "PMID": 971184}
{"id": "pubmed23n0032_8419", "title": "Antiarrhythmic effect of solvents: propylene glycol, benzyl alcohol.", "content": "Propylene glycol and benzyl alcohol, the main constituents of most solvent vehicles, display a pronounced antiarrhythmic-antifibrillatory effects, when injected intravenously into animals (dogs, rats) with spontaneous or drug-induced arrhythmias. The antiarrhythmic dose for propylene glycol amounts to 0.2-0.3 ml/kg of a 70 per cent solution and, for benzyl alcohol to 0.2-0.4 ml/kg of a 4 per cent solution in physiologic saline, respectively. Similar effects were also obtained by the combined injection of propylene glycol + benzyl alcohol, in proportions which correspond to the formulae of numerous commercial \"solvents\" (vehicles): 2 to 20 per cent solutions of benzyl alcohol in 70 per cent propylene glycol (0.05-0.2 ml/kg). The mechanisms which might be responsible for the antiarrhythmic activity of solvents are discussed: lengthening of the effective refractory period, local and general anaesthetic effects, changes of osmolarity. The intravenous injection of propylene glycol and/or benzyl alcohol, in high doses, produces intravascular haemolysis. Clinical investigations are recommended as to the potential, beneficial or toxic effects of drug solvents, especially upon the cardiocirculatory system.", "contents": "Antiarrhythmic effect of solvents: propylene glycol, benzyl alcohol. Propylene glycol and benzyl alcohol, the main constituents of most solvent vehicles, display a pronounced antiarrhythmic-antifibrillatory effects, when injected intravenously into animals (dogs, rats) with spontaneous or drug-induced arrhythmias. The antiarrhythmic dose for propylene glycol amounts to 0.2-0.3 ml/kg of a 70 per cent solution and, for benzyl alcohol to 0.2-0.4 ml/kg of a 4 per cent solution in physiologic saline, respectively. Similar effects were also obtained by the combined injection of propylene glycol + benzyl alcohol, in proportions which correspond to the formulae of numerous commercial \"solvents\" (vehicles): 2 to 20 per cent solutions of benzyl alcohol in 70 per cent propylene glycol (0.05-0.2 ml/kg). The mechanisms which might be responsible for the antiarrhythmic activity of solvents are discussed: lengthening of the effective refractory period, local and general anaesthetic effects, changes of osmolarity. The intravenous injection of propylene glycol and/or benzyl alcohol, in high doses, produces intravascular haemolysis. Clinical investigations are recommended as to the potential, beneficial or toxic effects of drug solvents, especially upon the cardiocirculatory system.", "PMID": 971216}
{"id": "pubmed23n0032_8420", "title": "[Recording of ventricular pressure by conventional catheter manometer systems. Efficiency of several combinations of conventional catheters, modern transducers and catheter-flush systems (author's transl)].", "content": "The experimentally in vitro determined dynamic response characteristics of 38 catheter manometer systems were uniform in the worst case to 5 c.p.s. and optimally to 26 c.p.s. Accordingly, some systems are only satisfactory for ordinary pressure recording in cardiac rest, while better systems record dp/dt correct up to moderate inotropic stimulation of the heart. In the frequency range of uniform response (amplitude error less +/- 5%) the phase distortion is also negligible. In clinical application the investigator is often restricted to special type of cardiac catheter. In this case a low compliant transducer yields superior results. In all examined systems the combination with MSD 10 transducers is best, whereas the combination with P 23 Db transducers leads to minimal results. An inadequate system for recording ventricular pressure pulses leads in most cases to overestimations of dp/dtmax. The use of low frequency pass filters to attenuate higher frequency artefacts is, under clinical conditions, not suitable for extending the range of uniform frequency response. The dynamic response of 14 catheter manometer systems with two types of continuous self flush units was determined. The use of the P 37 flush unit in combination with small internal diameter catheters leads to serious error in ordinary pressure recording, due to amplitude distortion of the lower harmonics. The frequency response characteristics of the combination of an Intraflow flush system and MSD 10 transducer was similar to the non-flushing P 23 Db transducer feature.", "contents": "[Recording of ventricular pressure by conventional catheter manometer systems. Efficiency of several combinations of conventional catheters, modern transducers and catheter-flush systems (author's transl)]. The experimentally in vitro determined dynamic response characteristics of 38 catheter manometer systems were uniform in the worst case to 5 c.p.s. and optimally to 26 c.p.s. Accordingly, some systems are only satisfactory for ordinary pressure recording in cardiac rest, while better systems record dp/dt correct up to moderate inotropic stimulation of the heart. In the frequency range of uniform response (amplitude error less +/- 5%) the phase distortion is also negligible. In clinical application the investigator is often restricted to special type of cardiac catheter. In this case a low compliant transducer yields superior results. In all examined systems the combination with MSD 10 transducers is best, whereas the combination with P 23 Db transducers leads to minimal results. An inadequate system for recording ventricular pressure pulses leads in most cases to overestimations of dp/dtmax. The use of low frequency pass filters to attenuate higher frequency artefacts is, under clinical conditions, not suitable for extending the range of uniform frequency response. The dynamic response of 14 catheter manometer systems with two types of continuous self flush units was determined. The use of the P 37 flush unit in combination with small internal diameter catheters leads to serious error in ordinary pressure recording, due to amplitude distortion of the lower harmonics. The frequency response characteristics of the combination of an Intraflow flush system and MSD 10 transducer was similar to the non-flushing P 23 Db transducer feature.", "PMID": 971217}
{"id": "pubmed23n0032_8421", "title": "Noncompetitive catecholamine-antagonism of acetylcholine in the sympathectomized mammalian ventricular myocardium.", "content": "The effect of the vagus transmitter on the ventricular myocardium varies qualitatively as well as quantitatively in different warm-blood species. In the ventricular myocardium of birds (duck, hen), a part of the acetylcholine sensitivity remains even after catecholamine depletion. In contrast, the inhibitory effects of acetylcholine on the ventricular myocardium of mammals (rat, guinea pig, cat, dog, man) depend on the activity of the sympathicus. In the chemically sympathectomized (6-OH-DA) cat ventricular myocardium, a cholinergic innervation is, histochemically, clearly demonstrable. Correspondingly significant effects of endogenous (i.e. releasable by field stimulation) and exogenous acetylcholine can be seen if the contraction force is enhanced by exogenous catecholamines. This preparation is, therefore, a good model for the demonstration of a catecholamine antagonism of acetylcholine. Using this model, on the basis of dose-response relationships, it is possible to demonstrate that a noncompetitive catecholamine-antagonism of acetylcholine indeed exists in the mammalian ventricular myocardium independent of a cholinergically mediated reduction in the norepinephrine release.", "contents": "Noncompetitive catecholamine-antagonism of acetylcholine in the sympathectomized mammalian ventricular myocardium. The effect of the vagus transmitter on the ventricular myocardium varies qualitatively as well as quantitatively in different warm-blood species. In the ventricular myocardium of birds (duck, hen), a part of the acetylcholine sensitivity remains even after catecholamine depletion. In contrast, the inhibitory effects of acetylcholine on the ventricular myocardium of mammals (rat, guinea pig, cat, dog, man) depend on the activity of the sympathicus. In the chemically sympathectomized (6-OH-DA) cat ventricular myocardium, a cholinergic innervation is, histochemically, clearly demonstrable. Correspondingly significant effects of endogenous (i.e. releasable by field stimulation) and exogenous acetylcholine can be seen if the contraction force is enhanced by exogenous catecholamines. This preparation is, therefore, a good model for the demonstration of a catecholamine antagonism of acetylcholine. Using this model, on the basis of dose-response relationships, it is possible to demonstrate that a noncompetitive catecholamine-antagonism of acetylcholine indeed exists in the mammalian ventricular myocardium independent of a cholinergically mediated reduction in the norepinephrine release.", "PMID": 971218}
{"id": "pubmed23n0032_8422", "title": "[Aberrant subclavian arteries: predominance in females (author's transl)].", "content": "Thirty-five cases of aberrant subclavian artery were observed in 1408 consecutive necropsies on fetuses, newborns, infants and children; the autopsies were performed by the same pathologist. The right subclavian artery was found to be involved in 32 cases, the left subclavian artery in 3 cases. The artery always passed behind the esophagus. In 12 of the cases, the aberrant subclavian artery occurred as an isolated anomaly. In the remainder of the cases the aberrant subclavian artery was associated with malformations of the heart and the great vessels; coexistent extracardial defects were also noted. Of the 35 cases 13 were males and 22 females. In reviewing the literature a series of 431 cases is presented. Of these cases the aberrant subclavian artery occurred in 177 males and 254 females. The predominance in females is statistically established.", "contents": "[Aberrant subclavian arteries: predominance in females (author's transl)]. Thirty-five cases of aberrant subclavian artery were observed in 1408 consecutive necropsies on fetuses, newborns, infants and children; the autopsies were performed by the same pathologist. The right subclavian artery was found to be involved in 32 cases, the left subclavian artery in 3 cases. The artery always passed behind the esophagus. In 12 of the cases, the aberrant subclavian artery occurred as an isolated anomaly. In the remainder of the cases the aberrant subclavian artery was associated with malformations of the heart and the great vessels; coexistent extracardial defects were also noted. Of the 35 cases 13 were males and 22 females. In reviewing the literature a series of 431 cases is presented. Of these cases the aberrant subclavian artery occurred in 177 males and 254 females. The predominance in females is statistically established.", "PMID": 971219}
{"id": "pubmed23n0032_8423", "title": "\"Non-condensed\" and \"condensed\" chromain in transitional cell carcinoma of the human urinary bladder.", "content": "The area and content of \"non-condensed\" and \"condensed\" chromatin in smeared Feulgen-stained malignant urothelial cells were determined by means of scanning-cytophotometry. The results were compared with those from similar measurements of benign human transitional epithelial cells. There was no difference between the relative area and content of \"non-condensed\" and \"condensed\" chromatin in cancer nuclei and normal urothelial nuclei as far as nuclei of the same size and ploidy class were considered. Within the same ploidy class the relative area and content of \"non-condensed\" chromatin increased with increasing nuclear size. As increased nuclear size within the same ploidy class is typical for most cancer cells, cancer specimens therefore contained relatively more \"non-condensed\" chromatin than normal urothelium. Analogously the relative values of \"condensed\" chromatin decreased in cancer specimens. Only in high-polyploid cancer cells, which occurred more frequently in undifferentiated tumours, a slight decrease of the relative area and content of \"non-condensed\" chromatin was observed as compared with well differentiated diploid tumour cells. It was in polyploid tumours that the absolute area and content of \"condensed\" chromatin was increased as compared with diploid normal urothelium. This means that the changes in \"non-condensed\" and \"condensed\" chromatin were primarily dependent on nuclear size and total chromatin content and were not found to be a characteristic of cancer nuclei as compared with control nuclei of the same size and ploidy. These findings differ from the results of biochemical analyses of heterochromatin both in cells during carcinogenesis and also in cancer cells, but are in agreement with qualitative and quantitative morphological studies of smeared cancer nuclei.", "contents": "\"Non-condensed\" and \"condensed\" chromain in transitional cell carcinoma of the human urinary bladder. The area and content of \"non-condensed\" and \"condensed\" chromatin in smeared Feulgen-stained malignant urothelial cells were determined by means of scanning-cytophotometry. The results were compared with those from similar measurements of benign human transitional epithelial cells. There was no difference between the relative area and content of \"non-condensed\" and \"condensed\" chromatin in cancer nuclei and normal urothelial nuclei as far as nuclei of the same size and ploidy class were considered. Within the same ploidy class the relative area and content of \"non-condensed\" chromatin increased with increasing nuclear size. As increased nuclear size within the same ploidy class is typical for most cancer cells, cancer specimens therefore contained relatively more \"non-condensed\" chromatin than normal urothelium. Analogously the relative values of \"condensed\" chromatin decreased in cancer specimens. Only in high-polyploid cancer cells, which occurred more frequently in undifferentiated tumours, a slight decrease of the relative area and content of \"non-condensed\" chromatin was observed as compared with well differentiated diploid tumour cells. It was in polyploid tumours that the absolute area and content of \"condensed\" chromatin was increased as compared with diploid normal urothelium. This means that the changes in \"non-condensed\" and \"condensed\" chromatin were primarily dependent on nuclear size and total chromatin content and were not found to be a characteristic of cancer nuclei as compared with control nuclei of the same size and ploidy. These findings differ from the results of biochemical analyses of heterochromatin both in cells during carcinogenesis and also in cancer cells, but are in agreement with qualitative and quantitative morphological studies of smeared cancer nuclei.", "PMID": 971257}
{"id": "pubmed23n0032_8424", "title": "[Sarcoma 180: growth and regression. Comparative investigations using flow-through and scanning cytophotometers as well as histological, cytological and autoradiographic techniques (author's transl)].", "content": "The growth and the regression of the experimental tumor S 180 was investiaged by volume measurements and by cytophotometric studies on 50 mice in each of a pilot and in the present main experiment. In addition, histological, cytological and cytokinetic examinations were performed. Beginning on day 18, a spontaneous regression of the tumor was found in 20% and 38% of the animals, respectively. DNA-measurements were performed with a scanning-microspectrophotometer on tumor tissue imprints and tumor cell suspensions and were also carried out with a flow-through cytophotometer ICP on suspensions. DNA-histograms were plotted on days 4, 7, 12, 18, 20, 22, 26 and 29 after the transplantation. These revealed a constant position of the maxima at 2C for normal cells and at 4C and 8C for the tumor cells. In particular, by measuring a large total number of 4,968,000 nuclei with the ICP, distinct changes were found in the proportion of the single nuclei classes during growth and spontaneous regression. This technique also enables a quantitative measurement to be made of the DNA of cell debris from necrosis. With growing tumos, the proportion of the tumor cells increased to a maximum of 65% on day 18 and decreased to 37% on day 29. The DNA of cell debris grew from 8% to 47%. The proportion of normal cells was only 10% to 15% in the final phases. In tumors with a spontaneous regression the proportion of the tumor cell nuclei was 20% on day 18 and 11% on day 29. The proportion of the DNA of cell debris was again about 45%. The proportion of normal nuclei was greatly increased to 45%. Histological and cytological evidence together with measurements of the areas of nuclei and incorporation of 3H-TdR confirmed that the normal cells, the number of which increased during spontaneous regression were cells of a fibrovascular granulation tissue. While the rate of the DNA-synthesis of growing tumors continously decreased with age there was also a rapid decrease of the labelling index of spontaneously regressing tumors between days 12 and 18. In the final phase only the nuclei of the granulation tissue were labelled.", "contents": "[Sarcoma 180: growth and regression. Comparative investigations using flow-through and scanning cytophotometers as well as histological, cytological and autoradiographic techniques (author's transl)]. The growth and the regression of the experimental tumor S 180 was investiaged by volume measurements and by cytophotometric studies on 50 mice in each of a pilot and in the present main experiment. In addition, histological, cytological and cytokinetic examinations were performed. Beginning on day 18, a spontaneous regression of the tumor was found in 20% and 38% of the animals, respectively. DNA-measurements were performed with a scanning-microspectrophotometer on tumor tissue imprints and tumor cell suspensions and were also carried out with a flow-through cytophotometer ICP on suspensions. DNA-histograms were plotted on days 4, 7, 12, 18, 20, 22, 26 and 29 after the transplantation. These revealed a constant position of the maxima at 2C for normal cells and at 4C and 8C for the tumor cells. In particular, by measuring a large total number of 4,968,000 nuclei with the ICP, distinct changes were found in the proportion of the single nuclei classes during growth and spontaneous regression. This technique also enables a quantitative measurement to be made of the DNA of cell debris from necrosis. With growing tumos, the proportion of the tumor cells increased to a maximum of 65% on day 18 and decreased to 37% on day 29. The DNA of cell debris grew from 8% to 47%. The proportion of normal cells was only 10% to 15% in the final phases. In tumors with a spontaneous regression the proportion of the tumor cell nuclei was 20% on day 18 and 11% on day 29. The proportion of the DNA of cell debris was again about 45%. The proportion of normal nuclei was greatly increased to 45%. Histological and cytological evidence together with measurements of the areas of nuclei and incorporation of 3H-TdR confirmed that the normal cells, the number of which increased during spontaneous regression were cells of a fibrovascular granulation tissue. While the rate of the DNA-synthesis of growing tumors continously decreased with age there was also a rapid decrease of the labelling index of spontaneously regressing tumors between days 12 and 18. In the final phase only the nuclei of the granulation tissue were labelled.", "PMID": 971258}
{"id": "pubmed23n0032_8425", "title": "The dynamics of collagen deposition in liver damaged by CCl4.", "content": "The rates of synthesis and catabolism of collagen are determined in the livers of normal rats and of rats treated with carbon tetrachloride. Several experimental conditions are applied with the intention of investigating the dynamic processes separately, in order to determine the relevant values. The deposition of collagen in liver after damage by CCl4 is a consequence of the reduction of turnover with a simultaneous increase in collagen-production. Together with the increase in synthesis, which seems to be a consequence of a numerical increase in cells, there is a reduction in catabolism of collagen: the more severe the damage, the more marked is the decrease of specific catabolism. Collagen-deposition in the liver is thus the result of accumulation. The results indicate the possibility that collagen-catabolism is effected by enzymes produced by adjacent parenchymal cells, so that their destruction enhances the accumulation of collagen material. The findings mentioned above are discussed in the context of the general criteria of scar-formation and the repair of damaged tissues.", "contents": "The dynamics of collagen deposition in liver damaged by CCl4. The rates of synthesis and catabolism of collagen are determined in the livers of normal rats and of rats treated with carbon tetrachloride. Several experimental conditions are applied with the intention of investigating the dynamic processes separately, in order to determine the relevant values. The deposition of collagen in liver after damage by CCl4 is a consequence of the reduction of turnover with a simultaneous increase in collagen-production. Together with the increase in synthesis, which seems to be a consequence of a numerical increase in cells, there is a reduction in catabolism of collagen: the more severe the damage, the more marked is the decrease of specific catabolism. Collagen-deposition in the liver is thus the result of accumulation. The results indicate the possibility that collagen-catabolism is effected by enzymes produced by adjacent parenchymal cells, so that their destruction enhances the accumulation of collagen material. The findings mentioned above are discussed in the context of the general criteria of scar-formation and the repair of damaged tissues.", "PMID": 971259}
{"id": "pubmed23n0032_8426", "title": "[Benign solid teratoma of the uterus (author's transl)].", "content": "On curettage, done in a 39 year old patient because of metorrhagia, a peanut-sized nodule was scraped away from the endometrium. Histologically it consisted of a jumble of irregularly shaped portions of normal appearing embryonal cartilage, of tortuous thick-walled vessels, of twisted bundles of nerves of ducts of respiratory and intestinal epithelium, and of sheets of hornifying skin. The haphazard disarray of tissues supported the notion that the lesion was not a remnant of a malformed embryo. Step-sections through the lesion revealed no traces of placental villi or decidua in the surrounding endometrium. The following were considered as possible origins of the teratoma: 1. displaced germinal cells, 2. retention of pluripotent m\u00fcllerian epithelium, 3. overaged or blighted ovum. The latter seems most compatible with the histological findings.", "contents": "[Benign solid teratoma of the uterus (author's transl)]. On curettage, done in a 39 year old patient because of metorrhagia, a peanut-sized nodule was scraped away from the endometrium. Histologically it consisted of a jumble of irregularly shaped portions of normal appearing embryonal cartilage, of tortuous thick-walled vessels, of twisted bundles of nerves of ducts of respiratory and intestinal epithelium, and of sheets of hornifying skin. The haphazard disarray of tissues supported the notion that the lesion was not a remnant of a malformed embryo. Step-sections through the lesion revealed no traces of placental villi or decidua in the surrounding endometrium. The following were considered as possible origins of the teratoma: 1. displaced germinal cells, 2. retention of pluripotent m\u00fcllerian epithelium, 3. overaged or blighted ovum. The latter seems most compatible with the histological findings.", "PMID": 971260}
{"id": "pubmed23n0032_8427", "title": "Polymorphism and predictability at the alpha-glycerophosphate dehydrogenase locus in Colias butterflies: gradients in allele frequency within single populations.", "content": "Heterozygosity at the alpha-glycerophosphate dehydrogenase locus of five species of Colias butterflies is widespread in montane populations; alpine and lowland populations are not heterozygous. Within a single demographically characterized population of C. meadii where the population extends from alpine down into montane habitats, a marked cline in allele frequency is seen. Such within-population clines suggest the involvement of strong selection across the marked ecological interface. Thermal factors are the most likely causative agents, but associative overdominance is not excluded.", "contents": "Polymorphism and predictability at the alpha-glycerophosphate dehydrogenase locus in Colias butterflies: gradients in allele frequency within single populations. Heterozygosity at the alpha-glycerophosphate dehydrogenase locus of five species of Colias butterflies is widespread in montane populations; alpine and lowland populations are not heterozygous. Within a single demographically characterized population of C. meadii where the population extends from alpine down into montane habitats, a marked cline in allele frequency is seen. Such within-population clines suggest the involvement of strong selection across the marked ecological interface. Thermal factors are the most likely causative agents, but associative overdominance is not excluded.", "PMID": 971290}
{"id": "pubmed23n0032_8428", "title": "Genetic control of LDH isozymes in the house fly, Musca domestica.", "content": "Electrophoretic variations in lactate dehydrogenase from adult whole body homogenates are described for three laboratory strains of house flies, Musca domestica. Several crosses between different electrophoretic forms provided evidence that the observed variations are due to segregation of alleles at two distinct loci (designated as A and B loci) and that the LDH isozymes of house flies are dimers formed by a random association of subunits controlled by the two loci.", "contents": "Genetic control of LDH isozymes in the house fly, Musca domestica. Electrophoretic variations in lactate dehydrogenase from adult whole body homogenates are described for three laboratory strains of house flies, Musca domestica. Several crosses between different electrophoretic forms provided evidence that the observed variations are due to segregation of alleles at two distinct loci (designated as A and B loci) and that the LDH isozymes of house flies are dimers formed by a random association of subunits controlled by the two loci.", "PMID": 971291}
{"id": "pubmed23n0032_8429", "title": "Patterns of molecular variation. I. Interspecific comparisons of electromorphs in the Drosophila mulleri complex.", "content": "The average mobility of electromorphs at an enzyme locus in a single population was defined as the weighted average mobility of the electromorphs in that population, where the electromorph frequencies are the weights. A derivative distance measure was defined whose taxonomic utility was determined in the Drosophila mulleri species complex. Most of the variation in this metric was at the interspecific level, primarily among clusters of sibling species. The electromorphs of some loci were equally and regularly spaced, while those of other loci were less regular in their spacing. Overall, these minor perturbations from regular spacing did not noticeably detract from the taxonomic utility of average mobility, and cluster analysis yielded the same taxonomic relationships as more conventional nonmolecular treatments. On the other hand, electromorph spacing may be related to functional constraints on the enzyme molecules. Some possible implications of the results for the modes of selection during evolution of the different enzymes are discussed.", "contents": "Patterns of molecular variation. I. Interspecific comparisons of electromorphs in the Drosophila mulleri complex. The average mobility of electromorphs at an enzyme locus in a single population was defined as the weighted average mobility of the electromorphs in that population, where the electromorph frequencies are the weights. A derivative distance measure was defined whose taxonomic utility was determined in the Drosophila mulleri species complex. Most of the variation in this metric was at the interspecific level, primarily among clusters of sibling species. The electromorphs of some loci were equally and regularly spaced, while those of other loci were less regular in their spacing. Overall, these minor perturbations from regular spacing did not noticeably detract from the taxonomic utility of average mobility, and cluster analysis yielded the same taxonomic relationships as more conventional nonmolecular treatments. On the other hand, electromorph spacing may be related to functional constraints on the enzyme molecules. Some possible implications of the results for the modes of selection during evolution of the different enzymes are discussed.", "PMID": 971292}
{"id": "pubmed23n0032_8430", "title": "Esterases in the mosquito Culex pipiens pipiens L: formal genetics and polymorphism of adult esterases.", "content": "The genetics of two esterase loci active in autogenous adults of the mosquito Culex pipiens pipiens L. has been studied by means of starch gel electrophoresis. Three alleles at the Est-1 locus and eight at the Est-2 locus are described. Both loci have a null allele. Active alleles are codominant and there is no hybrid enzyme in heterozygotes. The Est-1 locus codes esterases preferentially hydrolyzing alpha-naphthylacetate and the Est-2 locus esterases preferentially hydrolyzing beta-naphthylacetate. Strains homozygous for both loci were selected. Linkage studies of the two loci have shown that they are not sex linked but are linked to each other, the crossover frequency being 8.6%. The polymorphism of two laboratory and two natural populations is described for both loci. Phenotypic distributions are in good agreement with Hardy-Weinberg expectations.", "contents": "Esterases in the mosquito Culex pipiens pipiens L: formal genetics and polymorphism of adult esterases. The genetics of two esterase loci active in autogenous adults of the mosquito Culex pipiens pipiens L. has been studied by means of starch gel electrophoresis. Three alleles at the Est-1 locus and eight at the Est-2 locus are described. Both loci have a null allele. Active alleles are codominant and there is no hybrid enzyme in heterozygotes. The Est-1 locus codes esterases preferentially hydrolyzing alpha-naphthylacetate and the Est-2 locus esterases preferentially hydrolyzing beta-naphthylacetate. Strains homozygous for both loci were selected. Linkage studies of the two loci have shown that they are not sex linked but are linked to each other, the crossover frequency being 8.6%. The polymorphism of two laboratory and two natural populations is described for both loci. Phenotypic distributions are in good agreement with Hardy-Weinberg expectations.", "PMID": 971293}
{"id": "pubmed23n0032_8431", "title": "Autosomal genetic control of the activity of a new variant ornithine transcarbamylase in chicken kidney.", "content": "The mode of inheritance of the gene for chick kidney ornithine transcarbamylase (OTC), found previously as a genetic variant, was investigated. White Leghorn B line males homozygous for the allele for the variant OTC gene were selected using the California Gray breed, having a near-absolute deficiency of the enzyme. Then further crosses of the two breeds were made. The mean value of the OTC level of F1 progeny was about 170 units. Chicks from the backcross generation were divided into two groups, of high activity and low activity, in a ratio of 1:1. F2 chicks were divided into three groups: one-fourth of the chicks were classified as a \"super high\" group, one-half were \"high,\" and the remaining one-fourth were \"low\"; the mean values for OTC level were 356.7, 196.4, and 15.6 units, respectively. From these results, it was suggested that the variant OTC represents a simple autosomal incompletely dominant trait.", "contents": "Autosomal genetic control of the activity of a new variant ornithine transcarbamylase in chicken kidney. The mode of inheritance of the gene for chick kidney ornithine transcarbamylase (OTC), found previously as a genetic variant, was investigated. White Leghorn B line males homozygous for the allele for the variant OTC gene were selected using the California Gray breed, having a near-absolute deficiency of the enzyme. Then further crosses of the two breeds were made. The mean value of the OTC level of F1 progeny was about 170 units. Chicks from the backcross generation were divided into two groups, of high activity and low activity, in a ratio of 1:1. F2 chicks were divided into three groups: one-fourth of the chicks were classified as a \"super high\" group, one-half were \"high,\" and the remaining one-fourth were \"low\"; the mean values for OTC level were 356.7, 196.4, and 15.6 units, respectively. From these results, it was suggested that the variant OTC represents a simple autosomal incompletely dominant trait.", "PMID": 971294}
{"id": "pubmed23n0032_8432", "title": "Water flux through porcine aortic tissue due to a hydrostatic pressure gradient.", "content": "The water flux through preparations of porcine aorta has been investigated. After an initial unsteady period a stable flux of approximately 2 mul/cm2-h was reached and this flux rate remained constant for several hours. Under the experimental conditions which were maintained (110 mm Hg pressure drop across a 2 mm thick section of tissue) this total flux corresponds to a hydraulic conductivity of less than or equal to 7.0 X 10(-13) cm4/dyne-sec. Since these data were obtained in tissue samples where the endothelial layer was not intact, they represent values which are in fact larger than the actual in vivo condition where the endothelial barrier would serve as an additional resistance. Thus, they demonstrate that the transmural flux of water across the aorta wall in vitro due to a pressure gradient is extremely small and, therefore, that other mass transfer mechanisms may be significant.", "contents": "Water flux through porcine aortic tissue due to a hydrostatic pressure gradient. The water flux through preparations of porcine aorta has been investigated. After an initial unsteady period a stable flux of approximately 2 mul/cm2-h was reached and this flux rate remained constant for several hours. Under the experimental conditions which were maintained (110 mm Hg pressure drop across a 2 mm thick section of tissue) this total flux corresponds to a hydraulic conductivity of less than or equal to 7.0 X 10(-13) cm4/dyne-sec. Since these data were obtained in tissue samples where the endothelial layer was not intact, they represent values which are in fact larger than the actual in vivo condition where the endothelial barrier would serve as an additional resistance. Thus, they demonstrate that the transmural flux of water across the aorta wall in vitro due to a pressure gradient is extremely small and, therefore, that other mass transfer mechanisms may be significant.", "PMID": 971339}
{"id": "pubmed23n0032_8433", "title": "Treatment of type II hyperlipoproteinemia with d-thyroxine.", "content": "The effectiveness of a new, almost l-thyroxine free preparation of d-thyroxine (Dynothel) was tested in 15 patients with Type IIa and 4 patients with Type IIb hyperlipoproteinemia. Eleven patients with Type IIa and 3 with Type IIb were responsive to treatment and showed an average 26% decrease in plasma TC. This decrement in plasma TC was mirrored in a significant reduction of LDL cholesterol in Type IIa and IIb. While VLDL cholesterol slightly decrease in Type IIb, it remained the same in Type IIa and so did the HDL cholesterol in both types. As neither VLDL nor LDL or HDL triglyceride levels changed very much in either type, the total plasma triglycerides remained the same. The plasma phospholipids were higher in Type IIa and lower in Type IIb on therapy. Thus, Dynothel seems to be a potent d-thyroxine preparation for lowering plasma cholesterol, this decrease being brought about by reduction of LDL cholesterol levels. The effect of the drug on plasma TG and PL is less certain.", "contents": "Treatment of type II hyperlipoproteinemia with d-thyroxine. The effectiveness of a new, almost l-thyroxine free preparation of d-thyroxine (Dynothel) was tested in 15 patients with Type IIa and 4 patients with Type IIb hyperlipoproteinemia. Eleven patients with Type IIa and 3 with Type IIb were responsive to treatment and showed an average 26% decrease in plasma TC. This decrement in plasma TC was mirrored in a significant reduction of LDL cholesterol in Type IIa and IIb. While VLDL cholesterol slightly decrease in Type IIb, it remained the same in Type IIa and so did the HDL cholesterol in both types. As neither VLDL nor LDL or HDL triglyceride levels changed very much in either type, the total plasma triglycerides remained the same. The plasma phospholipids were higher in Type IIa and lower in Type IIb on therapy. Thus, Dynothel seems to be a potent d-thyroxine preparation for lowering plasma cholesterol, this decrease being brought about by reduction of LDL cholesterol levels. The effect of the drug on plasma TG and PL is less certain.", "PMID": 971340}
{"id": "pubmed23n0032_8434", "title": "Number, location and severity of coronary arterial changes in steelhead trout (Salmo gairdnerii).", "content": "Steelhead trout (Salmo gairdnerii) were taken at three stages of sexual maturity to study their coronary arteries for arteriosclerotic lesions. At least 18 sections from the glutaraldehyde- and osmium-fixed arteries were obtained from each fish. Fish in the middle of the spawning migration and sexually mature fish at the spawning ground had lesions in about 20% of the arterial sections. These consisted of focal proliferations of smooth muscle cells projecting into the lumen through the broken elastic lamina with an intact endothelium around them. Sexually mature fish with patches of fungus on their head and back had twice as high a percentage of arterial sections with lesions as the first two groups of fish. Sexually immature fish were not studied. The lesions occurred approximately equally in all sizes of coronary arteries except for very small arteries. All lesions but one were focal; that lesion involved a third of the intima and the media. The lesions have no elastic lamina below the endothelium and seem to have no lipid.", "contents": "Number, location and severity of coronary arterial changes in steelhead trout (Salmo gairdnerii). Steelhead trout (Salmo gairdnerii) were taken at three stages of sexual maturity to study their coronary arteries for arteriosclerotic lesions. At least 18 sections from the glutaraldehyde- and osmium-fixed arteries were obtained from each fish. Fish in the middle of the spawning migration and sexually mature fish at the spawning ground had lesions in about 20% of the arterial sections. These consisted of focal proliferations of smooth muscle cells projecting into the lumen through the broken elastic lamina with an intact endothelium around them. Sexually mature fish with patches of fungus on their head and back had twice as high a percentage of arterial sections with lesions as the first two groups of fish. Sexually immature fish were not studied. The lesions occurred approximately equally in all sizes of coronary arteries except for very small arteries. All lesions but one were focal; that lesion involved a third of the intima and the media. The lesions have no elastic lamina below the endothelium and seem to have no lipid.", "PMID": 971341}
{"id": "pubmed23n0032_8435", "title": "Hepatic cholesterol-7alpha-hydroxylase activity in neurogenic hypercholesterolemia.", "content": "The cholesterol-7alpha-hydroxylase activity of hepatic microsomal preparations of hypothalamic hypercholesterolemic rats and normal rats was assayed in rats fed diets high and low in cholesterol, and in rats killed at the supposed height and at the nadir of the diurnal cycle of enzyme activity. The activity of this enzyme system appeared to be unimpaired in the hypothalamic hypercholesterolemic rat.", "contents": "Hepatic cholesterol-7alpha-hydroxylase activity in neurogenic hypercholesterolemia. The cholesterol-7alpha-hydroxylase activity of hepatic microsomal preparations of hypothalamic hypercholesterolemic rats and normal rats was assayed in rats fed diets high and low in cholesterol, and in rats killed at the supposed height and at the nadir of the diurnal cycle of enzyme activity. The activity of this enzyme system appeared to be unimpaired in the hypothalamic hypercholesterolemic rat.", "PMID": 971342}
{"id": "pubmed23n0032_8436", "title": "Treatment of hypercholesterolemia with Secholex. A long-term clinical trial and comparison with cholestyramine.", "content": "The efficacy of an anion-exchange gel, Secholex, as a hypocholesterolemic agent was assessed in 46 patients in 4 different studies and the effects were compared with those of cholestyramine. All patients had severe Type II-a or II-b hyperlipoproteinemia. In short-term metabolic studies Secholex (15 g/day) and cholestyramine (16 g/day) decreased serum cholesterol levels and increased total fecal sterol output and serum methyl sterol concentration to a similar extent, but cholestyramine was more effective than Secholex in increasing fecal bile acid excretion. In crossover studies, the two drugs appeared to be equally effective in lowing serum cholesterol levels but the patients mostly preferred Secholex. Twenty patients were treated with Secholex over a two-year period. The average decrease in serum cholesterol levels from the mean pretreatment value of 406 mg/100 ml was 15% during the first year, and 13% during the second year. In 5 patients the serum cholesterol was permanently lowered by more than 20% (good responders), while in 7 patients the average reduction of serum cholesterol level during Secholex administration was less than 10% (non-responders). The serum triglyceride level was slightly decreased by Secholex in Type II-b patients but was unaltered in Type II-a patients. At the end of the treatment period, serum iron and vitamin B12 levels were normal but the serum folic acid concentration was reduced in eight of 20 patients. A dose--response study indicated that a similar cholesterol-lowering effect was obtained with daily doses of 9 and 15 g of Secholex. It is concluded that Secholex is a relatively safe drug which effectively reduces serum cholesterol levels in two-thirds of patients with severe hypercholesterolemia.", "contents": "Treatment of hypercholesterolemia with Secholex. A long-term clinical trial and comparison with cholestyramine. The efficacy of an anion-exchange gel, Secholex, as a hypocholesterolemic agent was assessed in 46 patients in 4 different studies and the effects were compared with those of cholestyramine. All patients had severe Type II-a or II-b hyperlipoproteinemia. In short-term metabolic studies Secholex (15 g/day) and cholestyramine (16 g/day) decreased serum cholesterol levels and increased total fecal sterol output and serum methyl sterol concentration to a similar extent, but cholestyramine was more effective than Secholex in increasing fecal bile acid excretion. In crossover studies, the two drugs appeared to be equally effective in lowing serum cholesterol levels but the patients mostly preferred Secholex. Twenty patients were treated with Secholex over a two-year period. The average decrease in serum cholesterol levels from the mean pretreatment value of 406 mg/100 ml was 15% during the first year, and 13% during the second year. In 5 patients the serum cholesterol was permanently lowered by more than 20% (good responders), while in 7 patients the average reduction of serum cholesterol level during Secholex administration was less than 10% (non-responders). The serum triglyceride level was slightly decreased by Secholex in Type II-b patients but was unaltered in Type II-a patients. At the end of the treatment period, serum iron and vitamin B12 levels were normal but the serum folic acid concentration was reduced in eight of 20 patients. A dose--response study indicated that a similar cholesterol-lowering effect was obtained with daily doses of 9 and 15 g of Secholex. It is concluded that Secholex is a relatively safe drug which effectively reduces serum cholesterol levels in two-thirds of patients with severe hypercholesterolemia.", "PMID": 971343}
{"id": "pubmed23n0032_8437", "title": "Effect of the phospholipid vehicle on the transport of cholesterol in rats.", "content": "Rats were injected intravenously with liposomes made of [4(-14)C] cholesterol with [32P]lysolecithin, or [4(-14)C]cholesterol with [32P]lecithin. The clearance of both radioactive labels from plasma was observed, as well as their distribution in the organs after 15 and 60 min. At the same time, the esterification of injected [14C]cholesterol and the conversion of [32P]lysolecithin to [32P]lecithin and vice versa were examined. [14C]Cholesterol administered with lysolecithin was cleared from the plasma at a higher rate than with lecithin. Consequently the radioactivity of [14C]cholesterol in the aorta, heart, lung, kidney and liver changed with the applied phospholipid; with lysolecithin it was higher than with lecithin. Lysolecithin itself was distributed among the organs more evenly than lecithin, which accumulated most in the liver. If administered with lysolecithin, [14C]cholesterol was esterified in the plasma in a significantly higher proportion than if administered with lecithin. The antiatherogenous effect of lecithin and the atherogenous effect of lysolecithin are considered on the basis of different transport properties of these phospholipids.", "contents": "Effect of the phospholipid vehicle on the transport of cholesterol in rats. Rats were injected intravenously with liposomes made of [4(-14)C] cholesterol with [32P]lysolecithin, or [4(-14)C]cholesterol with [32P]lecithin. The clearance of both radioactive labels from plasma was observed, as well as their distribution in the organs after 15 and 60 min. At the same time, the esterification of injected [14C]cholesterol and the conversion of [32P]lysolecithin to [32P]lecithin and vice versa were examined. [14C]Cholesterol administered with lysolecithin was cleared from the plasma at a higher rate than with lecithin. Consequently the radioactivity of [14C]cholesterol in the aorta, heart, lung, kidney and liver changed with the applied phospholipid; with lysolecithin it was higher than with lecithin. Lysolecithin itself was distributed among the organs more evenly than lecithin, which accumulated most in the liver. If administered with lysolecithin, [14C]cholesterol was esterified in the plasma in a significantly higher proportion than if administered with lecithin. The antiatherogenous effect of lecithin and the atherogenous effect of lysolecithin are considered on the basis of different transport properties of these phospholipids.", "PMID": 971344}
{"id": "pubmed23n0032_8438", "title": "Incorporation in vitro of 14C-labelled acetate and 32P-labelled phosphate into lipid in thoracic aortae from hypertensive and nomotensive rabbits.", "content": "The effect of prior hypertension on lipid synthesis in the thoracic aortae of normal-fed and cholesterol-fed rabbits was studied in vitro using[1(-14)C] acetate and [32P] phosphate as lipid precursors. In normally fed rabbits, prior hypertension did not increase the incorporation of the labelled precursors into either phospholipid or neutral lipid. In cholesterol-fed rabbits, hypertension increased the incorporation of [32P] phosphate into phosphatidyl-choline and of [1(-14)C-acetate into cholesterol ester. The increased incorporation of [1(-14)C] acetate into cholesterol ester was accompanied by an increase in intimal total cholesterol concentration. For both normotensive and hypertensive cholesterol-fed rabbits there was a close correlation between cholesterol esterification and total cholesterol concentration of the thoracic intima. It is concluded that the increase in aortic lipid synthesis in hypertensive cholesterol-fed rabbits is secondary to the increased cholesterol accumulation induced by hypertension rather than to a direct stimulation of arterial wall lipid synthesis by hypertension per se.", "contents": "Incorporation in vitro of 14C-labelled acetate and 32P-labelled phosphate into lipid in thoracic aortae from hypertensive and nomotensive rabbits. The effect of prior hypertension on lipid synthesis in the thoracic aortae of normal-fed and cholesterol-fed rabbits was studied in vitro using[1(-14)C] acetate and [32P] phosphate as lipid precursors. In normally fed rabbits, prior hypertension did not increase the incorporation of the labelled precursors into either phospholipid or neutral lipid. In cholesterol-fed rabbits, hypertension increased the incorporation of [32P] phosphate into phosphatidyl-choline and of [1(-14)C-acetate into cholesterol ester. The increased incorporation of [1(-14)C] acetate into cholesterol ester was accompanied by an increase in intimal total cholesterol concentration. For both normotensive and hypertensive cholesterol-fed rabbits there was a close correlation between cholesterol esterification and total cholesterol concentration of the thoracic intima. It is concluded that the increase in aortic lipid synthesis in hypertensive cholesterol-fed rabbits is secondary to the increased cholesterol accumulation induced by hypertension rather than to a direct stimulation of arterial wall lipid synthesis by hypertension per se.", "PMID": 971345}
{"id": "pubmed23n0032_8439", "title": "Ischaemic disease in men and women with familial hypercholesterolaemia and xanthomatosis. A comparative study of genetic and environmental factors in 274 heterozygous cases.", "content": "The incidence of ischaemic diseases in familial hypercholesterolaemia and xanthomatosis (familial Type II) was studied in a group of 158 men and 116 women. (1) Men and women did not differ with regard to the inherited metabolic disease. Levels of serum cholesterol, the marker of the genetic defect, were not statistically different, and cholesterol deposition in tissues, visualized by skin tendon xanthomas, was not sex related. (2) Men and women were different with regard to ischaemic diseases. The incidence was much lower in women, and the mean age of onset 9 years later. Moreover, there was a sex difference in the nature of the ischaemic disease, with a high male predominance of myocardial infarction. (3) Since the major risk factor hypercholesterolaemia could not explain such a difference, the role of other risk factors was investigated. It was shown that the incidence of ischaemic diseases was increased in women by cigarette smoking and hypertension, and that the difference in age of onset between males and females was no longer seen in smoking women. It is suggested that the genetic factor is responsible for the atherosclerotic lesion in both sexes and that other factors playing a role in ischaemic complications including tobacco and hypertension may explain the difference between men and women.", "contents": "Ischaemic disease in men and women with familial hypercholesterolaemia and xanthomatosis. A comparative study of genetic and environmental factors in 274 heterozygous cases. The incidence of ischaemic diseases in familial hypercholesterolaemia and xanthomatosis (familial Type II) was studied in a group of 158 men and 116 women. (1) Men and women did not differ with regard to the inherited metabolic disease. Levels of serum cholesterol, the marker of the genetic defect, were not statistically different, and cholesterol deposition in tissues, visualized by skin tendon xanthomas, was not sex related. (2) Men and women were different with regard to ischaemic diseases. The incidence was much lower in women, and the mean age of onset 9 years later. Moreover, there was a sex difference in the nature of the ischaemic disease, with a high male predominance of myocardial infarction. (3) Since the major risk factor hypercholesterolaemia could not explain such a difference, the role of other risk factors was investigated. It was shown that the incidence of ischaemic diseases was increased in women by cigarette smoking and hypertension, and that the difference in age of onset between males and females was no longer seen in smoking women. It is suggested that the genetic factor is responsible for the atherosclerotic lesion in both sexes and that other factors playing a role in ischaemic complications including tobacco and hypertension may explain the difference between men and women.", "PMID": 971346}
{"id": "pubmed23n0032_8440", "title": "Comparative histological and morphometrical studies into the relevance of intimal thickening to coronary sclerosis.", "content": "It was found by comparative histological and morphometrical studies, carried out on 125 human hearts of different age (20-90 years) and sex, that there exist critical limiting ranges of intima thickness and the intima/media relationship (IMR) for diffuse intimal thickening and the preatheroma phase of arteriosclerosis. Since furthermore a maximum and a minimum intima thickness as relating to diffuse intimal thickening could be determined, it is assumed that the location and formation of arteriosclerotic plaques is determined by the degree of so-called diffuse intimal thickening. It follows from these findings that postnatal intimal proliferation represents a potential prearteriosclerotic lesion of the intima, and that progressive intimal thickening supports the arteriosclerotic alteration of the intima, leading, through an extensive necrosis of the intima, to the atheroma phase.", "contents": "Comparative histological and morphometrical studies into the relevance of intimal thickening to coronary sclerosis. It was found by comparative histological and morphometrical studies, carried out on 125 human hearts of different age (20-90 years) and sex, that there exist critical limiting ranges of intima thickness and the intima/media relationship (IMR) for diffuse intimal thickening and the preatheroma phase of arteriosclerosis. Since furthermore a maximum and a minimum intima thickness as relating to diffuse intimal thickening could be determined, it is assumed that the location and formation of arteriosclerotic plaques is determined by the degree of so-called diffuse intimal thickening. It follows from these findings that postnatal intimal proliferation represents a potential prearteriosclerotic lesion of the intima, and that progressive intimal thickening supports the arteriosclerotic alteration of the intima, leading, through an extensive necrosis of the intima, to the atheroma phase.", "PMID": 971347}
{"id": "pubmed23n0032_8441", "title": "Estrogen treatment and gonadal function in the regulation of lipoprotein lipase.", "content": "Lipoprotein lipase (LPL) activity was measured in adipose tissue, heart and diaphragm in Sprague--Dawley rats after estrogen therapy or orchiectomy. Enzyme activity was measured by incubation of tissue fragments with a triolein emulsion in the presence of serum and heparin. In confirmation of other work, depression of adipose tissue LPL followed estradiol treatment in pharmacologic or near-physiologic doses. Cardiac and diaphragmatic muscle LPL were increased. Estrogen-treated male animals showed growth retardation. However, they gained weight steadily and did not show significant differences in serum insulin, glucose of D-beta-hydroxybutyrate. The effects of estradiol in male animals were reversed by sequential fasting and re-feeding. At times during growth and aging in normal female rats, adipose tissue activity was decreased while cardiac and skeletal muscle activities were increased relative to males of the same age or body weight. Castration of male rats failed to reproduce the effect of estrogens on tissue lipoprotein lipase. These in vitro data suggest that exogenous estrogens may shift the flux of triglyceride fatty acids from storage in the adipose organ toward incorporation by muscle. These, and other data, raise the possibility that physiological estrogen secretion exerts a tonic influence over the synthesis and ultimate destination of triglyceride fatty acids.", "contents": "Estrogen treatment and gonadal function in the regulation of lipoprotein lipase. Lipoprotein lipase (LPL) activity was measured in adipose tissue, heart and diaphragm in Sprague--Dawley rats after estrogen therapy or orchiectomy. Enzyme activity was measured by incubation of tissue fragments with a triolein emulsion in the presence of serum and heparin. In confirmation of other work, depression of adipose tissue LPL followed estradiol treatment in pharmacologic or near-physiologic doses. Cardiac and diaphragmatic muscle LPL were increased. Estrogen-treated male animals showed growth retardation. However, they gained weight steadily and did not show significant differences in serum insulin, glucose of D-beta-hydroxybutyrate. The effects of estradiol in male animals were reversed by sequential fasting and re-feeding. At times during growth and aging in normal female rats, adipose tissue activity was decreased while cardiac and skeletal muscle activities were increased relative to males of the same age or body weight. Castration of male rats failed to reproduce the effect of estrogens on tissue lipoprotein lipase. These in vitro data suggest that exogenous estrogens may shift the flux of triglyceride fatty acids from storage in the adipose organ toward incorporation by muscle. These, and other data, raise the possibility that physiological estrogen secretion exerts a tonic influence over the synthesis and ultimate destination of triglyceride fatty acids.", "PMID": 971348}
{"id": "pubmed23n0032_8442", "title": "Chylomicron metabolism in rabbits fed diets with or without added cholesterol.", "content": "Intestinal lymph chylomicrons, isotopically labelled with radioactive triacylglycerol and cholesterol, were injected into normally fed and cholesterol-fed rabbits in order to establish the pattern of clearance of intestinal lipoproteins from the plasma. In normal rabbits the cholesterol moiety of chylomicrons was removed from the plasma less readily than triacylglycerol. In cholesterol-fed rabbits, the clearance of triacylglycerol was unaltered, but clearance of chylomicron cholesterol was further retarded. The majority of the injected lymph cholesterol was recovered in d less than 1.019 g/ml lipoproteins. These observations support the notions that clearance of chylomicron remnants is impaired in the rabbit, and that hypercholesterolaemia in the cholesterol-fed rabbit is due to an accumulation of chylomicron remnants in the plasma.", "contents": "Chylomicron metabolism in rabbits fed diets with or without added cholesterol. Intestinal lymph chylomicrons, isotopically labelled with radioactive triacylglycerol and cholesterol, were injected into normally fed and cholesterol-fed rabbits in order to establish the pattern of clearance of intestinal lipoproteins from the plasma. In normal rabbits the cholesterol moiety of chylomicrons was removed from the plasma less readily than triacylglycerol. In cholesterol-fed rabbits, the clearance of triacylglycerol was unaltered, but clearance of chylomicron cholesterol was further retarded. The majority of the injected lymph cholesterol was recovered in d less than 1.019 g/ml lipoproteins. These observations support the notions that clearance of chylomicron remnants is impaired in the rabbit, and that hypercholesterolaemia in the cholesterol-fed rabbit is due to an accumulation of chylomicron remnants in the plasma.", "PMID": 971349}
{"id": "pubmed23n0032_8443", "title": "Effect of dietary restriction on plasma cholesterol and cholesterol excretion in the White Carneau pigeon.", "content": "The effect of short-term dietary restriction on plasma cholesterol and cholesterol balance was examined in young While Carneau pigeons. Dietary restriction of 1 month increased plasma cholesterol significantly and subsequently decreased to initial levels at 3 months. The fecal excretion of neutral sterols was significantly reduced (P less than 0.001) at both 1 and 3 months following dietary restriction, while the fecal excretion of bile acids showed no significant changes.", "contents": "Effect of dietary restriction on plasma cholesterol and cholesterol excretion in the White Carneau pigeon. The effect of short-term dietary restriction on plasma cholesterol and cholesterol balance was examined in young While Carneau pigeons. Dietary restriction of 1 month increased plasma cholesterol significantly and subsequently decreased to initial levels at 3 months. The fecal excretion of neutral sterols was significantly reduced (P less than 0.001) at both 1 and 3 months following dietary restriction, while the fecal excretion of bile acids showed no significant changes.", "PMID": 971350}
{"id": "pubmed23n0032_8444", "title": "The effect of intermittent carbon monoxide exposure on experimental atherosclerosis in the rabbit.", "content": "(1) Twenty-four female New Zealand White rabbits were fed commercial diet plus 2% cholesterol. Twelve of these animals were exposed to carbon monoxide for 4 hours per day, seven days per week for 10 weeks. The carbon monoxide exposure was such that the mean blood carboxy-haemoglobin was raised to approximately 20% during each exposure period. Twelve control animals breathed atmospheric air under the same conditions of confinement as the carbon monoxide-exposed group. (2) No significant differences in the plasma levels of cholesterol, triglycerides or glutamate oxalacetate transaminase were observed between the two groups during the experiment. (3) When the animals were sacrificed at the end of the experiment no significant differences were observed between the two groups in the aortic content of triglycerides, cholesterol or phospholipids. (4) The extent of coronary artery atherosclerosis was statistically significantly higher in the carbon monoxide group than in the control group. (5) Ultracentrifugal analysis of plasma lipoproteins revealed that there was significantly more cholesterol in the d less than l.006 fraction from the CO-exposed rabbits. (6) These findings, are discussed with particular reference to the claim that the causal agent in tobacco smoke associated arterial disease is carbon monoxide.", "contents": "The effect of intermittent carbon monoxide exposure on experimental atherosclerosis in the rabbit. (1) Twenty-four female New Zealand White rabbits were fed commercial diet plus 2% cholesterol. Twelve of these animals were exposed to carbon monoxide for 4 hours per day, seven days per week for 10 weeks. The carbon monoxide exposure was such that the mean blood carboxy-haemoglobin was raised to approximately 20% during each exposure period. Twelve control animals breathed atmospheric air under the same conditions of confinement as the carbon monoxide-exposed group. (2) No significant differences in the plasma levels of cholesterol, triglycerides or glutamate oxalacetate transaminase were observed between the two groups during the experiment. (3) When the animals were sacrificed at the end of the experiment no significant differences were observed between the two groups in the aortic content of triglycerides, cholesterol or phospholipids. (4) The extent of coronary artery atherosclerosis was statistically significantly higher in the carbon monoxide group than in the control group. (5) Ultracentrifugal analysis of plasma lipoproteins revealed that there was significantly more cholesterol in the d less than l.006 fraction from the CO-exposed rabbits. (6) These findings, are discussed with particular reference to the claim that the causal agent in tobacco smoke associated arterial disease is carbon monoxide.", "PMID": 971351}
{"id": "pubmed23n0032_8445", "title": "Effects of clofibrate treatment on plasma triglyceride concentration, plasma post-heparin clearing factor lipase (lipoprotein lipase) activity and serum clearing factor lipase activating ability in maturity-onset diabetes.", "content": "The effects of clofibrate on plasma triglyceride concentration, plasma post-heparin clearing factor lipase activity and serum clearing factor lipase activating ability were studied in a group of maturity-onset diabetic patients. Significant falls in both triglyceride concentration and in activating ability occurred within 2 weeks of beginning clofibrate treatment and, when treatment was stopped after 4 weeks, these changes were reversed within a further 4 weeks. Plasma post-heparin clearing factor lipase activity, on the other hand, was significantly increased during clofibrate administration and fell again when the treatment was stopped. The possible interrelationships of these findings are discussed.", "contents": "Effects of clofibrate treatment on plasma triglyceride concentration, plasma post-heparin clearing factor lipase (lipoprotein lipase) activity and serum clearing factor lipase activating ability in maturity-onset diabetes. The effects of clofibrate on plasma triglyceride concentration, plasma post-heparin clearing factor lipase activity and serum clearing factor lipase activating ability were studied in a group of maturity-onset diabetic patients. Significant falls in both triglyceride concentration and in activating ability occurred within 2 weeks of beginning clofibrate treatment and, when treatment was stopped after 4 weeks, these changes were reversed within a further 4 weeks. Plasma post-heparin clearing factor lipase activity, on the other hand, was significantly increased during clofibrate administration and fell again when the treatment was stopped. The possible interrelationships of these findings are discussed.", "PMID": 971352}
{"id": "pubmed23n0032_8446", "title": "On the cholesteryl ester hydrolase activity in the microsomal and supernatant fractions of pigeon aorta.", "content": "Cholesteryl ester hydrolase activity was measured in the microsomal and supernatant fractions of the aorta of atherosclerosis-susceptible White Carneau and atherosclerosis-resistant Show Racer pigeons while on their normal cholesterol-free diets. Enzyme activities from both fractions showed fatty acid specificities for the hydrolysis of different cholesteryl esters in the following decreasing order: Linoleate greater than oleate greater than palmitate. At 9 months of age (the period of lipid accumulation) the microsomal enzyme activity in the Show Racer breed was significantly higher (P less than 0.001) than in the White Carneau breed, while the supernatant enzyme was slightly higher (P less than 0.05) in the White Carneaux at this age. In older birds (3 years of age) these differences in enzyme activities disappeared.", "contents": "On the cholesteryl ester hydrolase activity in the microsomal and supernatant fractions of pigeon aorta. Cholesteryl ester hydrolase activity was measured in the microsomal and supernatant fractions of the aorta of atherosclerosis-susceptible White Carneau and atherosclerosis-resistant Show Racer pigeons while on their normal cholesterol-free diets. Enzyme activities from both fractions showed fatty acid specificities for the hydrolysis of different cholesteryl esters in the following decreasing order: Linoleate greater than oleate greater than palmitate. At 9 months of age (the period of lipid accumulation) the microsomal enzyme activity in the Show Racer breed was significantly higher (P less than 0.001) than in the White Carneau breed, while the supernatant enzyme was slightly higher (P less than 0.05) in the White Carneaux at this age. In older birds (3 years of age) these differences in enzyme activities disappeared.", "PMID": 971353}
{"id": "pubmed23n0032_8447", "title": "Some metabolic relationships in young patients with ischemic heart disease.", "content": "The interrelationships between body weight, insulin secretion and serum lipids were studied in 40 young white patients (mean age 37 years) with established ischemic heart disease (IHD), living in Johannesburg. None was severely obese, hypertensive or overtly diabetic. In general, strong positive correlations were found between body weight and insulin concentrations and between insulin levels and fasting serum triglycerides. However, insulin levels were relatively low in 4 patients with marked hypertriglyceridemia (above 350 mg/dl). These data are consistent with the postulate that insulin promotes (hepatic) triglyceride synthesis, but when there is gross hypertriglyceridemia peripheral triglyceride clearance becomes defective. Insignificant correlations were observed between body weight and serum lipids and between cholesterol and other metabolic variables. We conclude that there is a sequential link between increasing body weight, insulin secretion and triglyceride levels in young patients with IHD, but that cholesterolemia is independent of this axis.", "contents": "Some metabolic relationships in young patients with ischemic heart disease. The interrelationships between body weight, insulin secretion and serum lipids were studied in 40 young white patients (mean age 37 years) with established ischemic heart disease (IHD), living in Johannesburg. None was severely obese, hypertensive or overtly diabetic. In general, strong positive correlations were found between body weight and insulin concentrations and between insulin levels and fasting serum triglycerides. However, insulin levels were relatively low in 4 patients with marked hypertriglyceridemia (above 350 mg/dl). These data are consistent with the postulate that insulin promotes (hepatic) triglyceride synthesis, but when there is gross hypertriglyceridemia peripheral triglyceride clearance becomes defective. Insignificant correlations were observed between body weight and serum lipids and between cholesterol and other metabolic variables. We conclude that there is a sequential link between increasing body weight, insulin secretion and triglyceride levels in young patients with IHD, but that cholesterolemia is independent of this axis.", "PMID": 971354}
{"id": "pubmed23n0032_8448", "title": "[The effects of Triton WR 1339 on the secretion of bile lipids in the rat].", "content": "Rats bearing a biliary fistula received i.v. a solution of Triton WR 1339. Bile and plasma lipid composition was studied every 2 hrs and compared to that of control rats injected with saline. Two hours after Triton injection a sharp decrease in the bile secretion of lecithins (-- 93%), cholesterol (-- 50%) and bile salts (-- 50%) was observed together with a fall in bile flow (-- 20%). Eight hours after Triton administration the biliary output of lecithins, cholesterol and bile salts was lowered to --73%, --50% and --34% respectively compared to control animals. At that time an accumulation of L.C.A.T. substrates (lecithins and cholesterol) was observed in the plasma of Triton group. These modifications of biliary lipids after inhibition of L.C.A.T. activity by Triton W 1339 could be the result of a decreased production of plasma lysolecithins and cholesterol esters suggesting that both lipids could be important precursors for the synthesis of bile constituents. Furthermore this support the view that the production by the liver of plasma and bile lipids follows two distinct pathways.", "contents": "[The effects of Triton WR 1339 on the secretion of bile lipids in the rat]. Rats bearing a biliary fistula received i.v. a solution of Triton WR 1339. Bile and plasma lipid composition was studied every 2 hrs and compared to that of control rats injected with saline. Two hours after Triton injection a sharp decrease in the bile secretion of lecithins (-- 93%), cholesterol (-- 50%) and bile salts (-- 50%) was observed together with a fall in bile flow (-- 20%). Eight hours after Triton administration the biliary output of lecithins, cholesterol and bile salts was lowered to --73%, --50% and --34% respectively compared to control animals. At that time an accumulation of L.C.A.T. substrates (lecithins and cholesterol) was observed in the plasma of Triton group. These modifications of biliary lipids after inhibition of L.C.A.T. activity by Triton W 1339 could be the result of a decreased production of plasma lysolecithins and cholesterol esters suggesting that both lipids could be important precursors for the synthesis of bile constituents. Furthermore this support the view that the production by the liver of plasma and bile lipids follows two distinct pathways.", "PMID": 971361}
{"id": "pubmed23n0032_8449", "title": "Roentgenological and endoscopic findings of malignant lymphoma.", "content": "Roentgenological and endoscopic characteristics of malignant lymphoma of the stomach were studied referring to the gross pathological appearance of the tumors. Based on the results obtained, we classified malignant lymphoma of the stomach into four types, i.e., superficial, ulcer, polypoid and giant fold types.", "contents": "Roentgenological and endoscopic findings of malignant lymphoma. Roentgenological and endoscopic characteristics of malignant lymphoma of the stomach were studied referring to the gross pathological appearance of the tumors. Based on the results obtained, we classified malignant lymphoma of the stomach into four types, i.e., superficial, ulcer, polypoid and giant fold types.", "PMID": 971362}
{"id": "pubmed23n0032_8450", "title": "Duplication of aortic wall seen by echocardiography.", "content": "In four patients, echocardiography showed duplication of an aortic wall echo. An aortic dissection was present in only one. Other causes for the echocardiographic pattern were abscess in the interventricular septum, mitral stenosis, and dilatation of the noncoronary sinus of Valsalva. Division of the echo from the mitral ring into two separate posterior aortic wall echoes was seen only in the patient with dissecting aneurysm; it is suggested that this appearance adds to the specificity of the finding of aortic wall duplication by echocardiography in the diagnosis of aortic dissection.", "contents": "Duplication of aortic wall seen by echocardiography. In four patients, echocardiography showed duplication of an aortic wall echo. An aortic dissection was present in only one. Other causes for the echocardiographic pattern were abscess in the interventricular septum, mitral stenosis, and dilatation of the noncoronary sinus of Valsalva. Division of the echo from the mitral ring into two separate posterior aortic wall echoes was seen only in the patient with dissecting aneurysm; it is suggested that this appearance adds to the specificity of the finding of aortic wall duplication by echocardiography in the diagnosis of aortic dissection.", "PMID": 971373}
{"id": "pubmed23n0032_8451", "title": "'Incessant' tachycardias in Wolff-Parkinson-White syndrome. II: Role of atypical cycle length dependency and nodal-his escape beats in initiating reciprocating tachycardias.", "content": "Descriptions of patients with the Wolff-Parkinson-White (WPW) syndrome and reciprocating tachycardia in whom the initiation of the arrhythmia depended neither on the occurrence of premature beats nor on antecedent cycle-length shortening are given. In 5 the occurrence of escape beats in the bundle of His, usually in the presence of sinoatrial disease, activated the tachycardia circuit, but in the other 2 there were unusual mechanisms related to bradycardia-dependent block in the anomalous pathway, and delayed response to shortening of the atrial cycle length, respectively. Careful assessment of such mechanisms is essential for the correct choice of antiarrhythmic prophylactic therapy.", "contents": "'Incessant' tachycardias in Wolff-Parkinson-White syndrome. II: Role of atypical cycle length dependency and nodal-his escape beats in initiating reciprocating tachycardias. Descriptions of patients with the Wolff-Parkinson-White (WPW) syndrome and reciprocating tachycardia in whom the initiation of the arrhythmia depended neither on the occurrence of premature beats nor on antecedent cycle-length shortening are given. In 5 the occurrence of escape beats in the bundle of His, usually in the presence of sinoatrial disease, activated the tachycardia circuit, but in the other 2 there were unusual mechanisms related to bradycardia-dependent block in the anomalous pathway, and delayed response to shortening of the atrial cycle length, respectively. Careful assessment of such mechanisms is essential for the correct choice of antiarrhythmic prophylactic therapy.", "PMID": 971374}
{"id": "pubmed23n0032_8452", "title": "Coronary artery disease in patients dying from cardiogenic shock or congestive heart failure in the setting of acute myocardial infarction.", "content": "Pathological findings in the heart and particularly in the coronary arteries are reported from 70 patients dying from pump failure after acute myocardial infarction. Fifty of the patients had died in cardiogenic shock, the remainder from refractory congestive heart failure. Three-vessel disease (greater than or equal to 75% occlusion) was present in 68 per cent of the group with cardiogenic shock but in only 35 per cent of those with fatal congestive heart failure (P less than 0-02). In both groups there was an almost equal incidence (84% for cardiogenic shock and 80% for congestive heart failure) of severe disease (greater than or equal to 75% occlusion) over a long segment of the left anterior descending artery. However, there were differences between the two groups regarding the involvement of the other coronary arteries. Whereas patients with cardiogenic shock generally showed severe disease over a long segment in all coronary arteries, in 60 per cent of those with congestive heart failure there was only local severe narrowing of the right coronary artery with little or no narrowing of the peripheral part. Similarly, 60 per cent of those with congestive heart failure had less than 75 per cent narrowing in the left circumflex artery. These anatomical findings may be of relevance with regard to desirability of acute coronary bypass surgery in patients with pump failure after acute myocardial infarction.", "contents": "Coronary artery disease in patients dying from cardiogenic shock or congestive heart failure in the setting of acute myocardial infarction. Pathological findings in the heart and particularly in the coronary arteries are reported from 70 patients dying from pump failure after acute myocardial infarction. Fifty of the patients had died in cardiogenic shock, the remainder from refractory congestive heart failure. Three-vessel disease (greater than or equal to 75% occlusion) was present in 68 per cent of the group with cardiogenic shock but in only 35 per cent of those with fatal congestive heart failure (P less than 0-02). In both groups there was an almost equal incidence (84% for cardiogenic shock and 80% for congestive heart failure) of severe disease (greater than or equal to 75% occlusion) over a long segment of the left anterior descending artery. However, there were differences between the two groups regarding the involvement of the other coronary arteries. Whereas patients with cardiogenic shock generally showed severe disease over a long segment in all coronary arteries, in 60 per cent of those with congestive heart failure there was only local severe narrowing of the right coronary artery with little or no narrowing of the peripheral part. Similarly, 60 per cent of those with congestive heart failure had less than 75 per cent narrowing in the left circumflex artery. These anatomical findings may be of relevance with regard to desirability of acute coronary bypass surgery in patients with pump failure after acute myocardial infarction.", "PMID": 971375}
{"id": "pubmed23n0032_8453", "title": "Diagnosis of endocardial cushion defect with cross-sectional and M-mode scanning echocardiography. Differentiation from secundum atrial septal defect.", "content": "Twelve cases of endocardial cushion defect were studied before and after operation with ultrasono-cardiotomography (tomography) cross-sectional echocardiography, two-dimensional echocardiography, B-scan echocardiography) and M-mode scan along a horizontal section of the heart. For comparison, 20 healthy subjects, 18 cases of mitral valvular disease, 4 cases of congestive cardiomyopathy, 1 case of partial anomalous pulmonary venous drainage, and 25 cases of atrial septal defect of secundum type were also examined with the same technique. In cases without cardiac malformation, the echo of the anterior mitral valve was usually continuous medially with that of the interatrial septum in the horizontal plane at the level of the membranous septum. This feature was clearly recorded in all cases with right heart enlargement. In ostium secundum atrial septal defect the echo of the anterior mitral valve continued into that of the interatrial septum. An echo interruption was shown, indicating the defect itself to be in the middle part of the interatrial septum. In all the cases of endocardial cushion defect which we examined discontinuity was shown between the echo of the anterior mitral valve and that of the interatrial septum. This discontinuity was interpreted as indicating the defect itself. The mitral valve ring echo was close to the basal end of that of the interventricular septum, possibly reflecting an abnormal attachment of the mitral valve. In all cases, after operation, the echo of the artificial interatrial septum was recorded, continuous with that of the anterior mitral valve. The features of the echocardiographic sweep from the anterior mitral valve to the interatrial septum were thus different in the three groups. These echocardiographic differences are thought to correspond to the anatomical differences between the normal, atrial septal defect of secundum type, and endocardial cushion defect, and are essential features differentiating them from each other.", "contents": "Diagnosis of endocardial cushion defect with cross-sectional and M-mode scanning echocardiography. Differentiation from secundum atrial septal defect. Twelve cases of endocardial cushion defect were studied before and after operation with ultrasono-cardiotomography (tomography) cross-sectional echocardiography, two-dimensional echocardiography, B-scan echocardiography) and M-mode scan along a horizontal section of the heart. For comparison, 20 healthy subjects, 18 cases of mitral valvular disease, 4 cases of congestive cardiomyopathy, 1 case of partial anomalous pulmonary venous drainage, and 25 cases of atrial septal defect of secundum type were also examined with the same technique. In cases without cardiac malformation, the echo of the anterior mitral valve was usually continuous medially with that of the interatrial septum in the horizontal plane at the level of the membranous septum. This feature was clearly recorded in all cases with right heart enlargement. In ostium secundum atrial septal defect the echo of the anterior mitral valve continued into that of the interatrial septum. An echo interruption was shown, indicating the defect itself to be in the middle part of the interatrial septum. In all the cases of endocardial cushion defect which we examined discontinuity was shown between the echo of the anterior mitral valve and that of the interatrial septum. This discontinuity was interpreted as indicating the defect itself. The mitral valve ring echo was close to the basal end of that of the interventricular septum, possibly reflecting an abnormal attachment of the mitral valve. In all cases, after operation, the echo of the artificial interatrial septum was recorded, continuous with that of the anterior mitral valve. The features of the echocardiographic sweep from the anterior mitral valve to the interatrial septum were thus different in the three groups. These echocardiographic differences are thought to correspond to the anatomical differences between the normal, atrial septal defect of secundum type, and endocardial cushion defect, and are essential features differentiating them from each other.", "PMID": 971376}
{"id": "pubmed23n0032_8454", "title": "Familial sinoatrial node dysfunction. Increased vagal tone a possible aetiology.", "content": "Clinical and electrophysiological studies of a 13-year-old boy with sinus bradycardia revealed sinus node dysfunction. Long-term follow-up data of members of his family indicated familial sinus node dysfucntion. Increased vagal tone was present in all patients. It is suggested that excessive vagal discharge for a pronlonged time may be the basic mechanism of sinus node dysfunction in these patients.", "contents": "Familial sinoatrial node dysfunction. Increased vagal tone a possible aetiology. Clinical and electrophysiological studies of a 13-year-old boy with sinus bradycardia revealed sinus node dysfunction. Long-term follow-up data of members of his family indicated familial sinus node dysfucntion. Increased vagal tone was present in all patients. It is suggested that excessive vagal discharge for a pronlonged time may be the basic mechanism of sinus node dysfunction in these patients.", "PMID": 971377}
{"id": "pubmed23n0032_8455", "title": "Surgical treatment of pulmonary atresia with intact ventricular septum.", "content": "Fifty-nine children with pulmonary atresia and intact ventricular septum underwent various forms of surgical treatment at the Hospital for Sick Children, Toronto, during 1950 to mid 1975. Twenty-three patients had pulmonary valvotomy, 15 direct, 2 indirect, and 6 both direct valvotomy and infundibulectomy. All died, 19 early and 4 late. Of 13 patients who received a systemic-pulmonary artery shunt, 4 combined with surgical atrial septectomy, there are only 2 long-term survivors both of whom were children who had had a Waterston anastomosis. Recently we have been treating infants with small right ventricles with balloon atrial septostomy at cardiac catherterization followed by a Potts anastomosis and pulmonary valvotomy. If the Potts anastomosis appears satisfactory the persistent ductus arteriosus is ligated. This scheme was used in 23 infants, with 4 early deaths and 2 late deaths. Of 17 survivors, further shunts were required in 4 children. One child has had a formal repair, with insertion of valves in both tricuspid and pulmonary areas. We believe that this operative combination of Potts anastomosis and pulmonary valvotomy offers the infant with pulmonary atresia and a small right ventricle a relatively low initial mortality and the possibility of right ventricular enlargement and subsequent repair.", "contents": "Surgical treatment of pulmonary atresia with intact ventricular septum. Fifty-nine children with pulmonary atresia and intact ventricular septum underwent various forms of surgical treatment at the Hospital for Sick Children, Toronto, during 1950 to mid 1975. Twenty-three patients had pulmonary valvotomy, 15 direct, 2 indirect, and 6 both direct valvotomy and infundibulectomy. All died, 19 early and 4 late. Of 13 patients who received a systemic-pulmonary artery shunt, 4 combined with surgical atrial septectomy, there are only 2 long-term survivors both of whom were children who had had a Waterston anastomosis. Recently we have been treating infants with small right ventricles with balloon atrial septostomy at cardiac catherterization followed by a Potts anastomosis and pulmonary valvotomy. If the Potts anastomosis appears satisfactory the persistent ductus arteriosus is ligated. This scheme was used in 23 infants, with 4 early deaths and 2 late deaths. Of 17 survivors, further shunts were required in 4 children. One child has had a formal repair, with insertion of valves in both tricuspid and pulmonary areas. We believe that this operative combination of Potts anastomosis and pulmonary valvotomy offers the infant with pulmonary atresia and a small right ventricle a relatively low initial mortality and the possibility of right ventricular enlargement and subsequent repair.", "PMID": 971378}
{"id": "pubmed23n0032_8456", "title": "The Wenckebach phenomenon between electric pacemaker and ventricle.", "content": "Two cases which exhibited a Wenckebach form of exit conduction between an electrical pacemaker and the ventricular myocardium are presented. This manifests with increasing latency (stimulus to QRS  intervals) and ultimate stimulus failure (block). The cycle then repeats itself. The QRS duration also increases progressively within each cycle. The phenomenon connotes an adverse prognosis. Several postulates regarding the mechanism are discussed.", "contents": "The Wenckebach phenomenon between electric pacemaker and ventricle. Two cases which exhibited a Wenckebach form of exit conduction between an electrical pacemaker and the ventricular myocardium are presented. This manifests with increasing latency (stimulus to QRS  intervals) and ultimate stimulus failure (block). The cycle then repeats itself. The QRS duration also increases progressively within each cycle. The phenomenon connotes an adverse prognosis. Several postulates regarding the mechanism are discussed.", "PMID": 971379}
{"id": "pubmed23n0032_8457", "title": "Quantitative radionuclide angiocardiography. Determination of left ventricular ejection fraction in children.", "content": "A method is described for measuring left ventricular ejection fraction which uses high frequency computer recording of gamma scintillation camera data and peripheral venous injectinon of technetium-99m as sodium pertechnetate. Data from mechanical model experiments are used to show feasibility of this method. A phantom experiment is described which was used to develop a technique for accurate delineation of the ventricular outline in the presence of background. The left ventricular ejection was measured in 12 patients by radionuclide angiocardiography and biplane cineangiography. Comparison of these two methods gave a correlation coefficient of 0-91. In addition, left ventricular ejection fraction was measured in 34 patients (aged 7 weeks to 18 years) without evidence of cardiac disease using the radionuclide method alone. Average ejection fractions of 0-66 and 0-70 were found for children over 2 years of age and children 2 years of age or younger, respectively. In addition, an interobseerver comparison study was performed with the data from 10 patients, and only small differences were noted (SD 0-025).", "contents": "Quantitative radionuclide angiocardiography. Determination of left ventricular ejection fraction in children. A method is described for measuring left ventricular ejection fraction which uses high frequency computer recording of gamma scintillation camera data and peripheral venous injectinon of technetium-99m as sodium pertechnetate. Data from mechanical model experiments are used to show feasibility of this method. A phantom experiment is described which was used to develop a technique for accurate delineation of the ventricular outline in the presence of background. The left ventricular ejection was measured in 12 patients by radionuclide angiocardiography and biplane cineangiography. Comparison of these two methods gave a correlation coefficient of 0-91. In addition, left ventricular ejection fraction was measured in 34 patients (aged 7 weeks to 18 years) without evidence of cardiac disease using the radionuclide method alone. Average ejection fractions of 0-66 and 0-70 were found for children over 2 years of age and children 2 years of age or younger, respectively. In addition, an interobseerver comparison study was performed with the data from 10 patients, and only small differences were noted (SD 0-025).", "PMID": 971380}
{"id": "pubmed23n0032_8458", "title": "Prevention of balloon damage during initiation of intra-aortic balloon counterpulsation.", "content": "Damage to an intra-aortic balloon occurred on two occasions in 93 insertion procedures. The complication was recognized before gas embolization occurred, and neither patient suffered ill effects. On both occasions the balloon was holed when pinched by artery forceps applied to the 'dacron' graft.", "contents": "Prevention of balloon damage during initiation of intra-aortic balloon counterpulsation. Damage to an intra-aortic balloon occurred on two occasions in 93 insertion procedures. The complication was recognized before gas embolization occurred, and neither patient suffered ill effects. On both occasions the balloon was holed when pinched by artery forceps applied to the 'dacron' graft.", "PMID": 971381}
{"id": "pubmed23n0032_8459", "title": "Haemolysis after mitral valve replacement with the Bj\u00f6rk-Shiley and the Lillehei-Kaster disc valve prosthesis.", "content": "The present study reports on the incidence and degree of intravascular haemolysis in 33 patients with Bj\u00f6rk-Shiley tilting disc prosthesis and in 34 patients with Lillehei-Kaster pivoting disc valve prosthesis in the mitral position examined 12 to 24 months after the operation. Serum haptoglobin, serum lactate dehydrogenase, serum bilirubin, and haemoglobin estimations were performed. Significant haemolysis was detected in 85 per cent of the patients. Haptoglobin was absent or reduced in 72 per cent, while raised values for serum lactate dehydrogenase were found in 43 per cent. The increase in lactate dehydrogenase was moderate and showed no correlation with blood flow through the prosthesis or with the gradient across the prosthesis. Most patients had normal haemoglobin and normal serum bilirubin values. Although intravascular haemolysis was of little clinical significance, the increase in lactate dehyrdrogenase was significantly higher in patients with the Lillehei-Kaster prosthesis than in patients with the Bj\u00f6rk Shiley prosthesis, indicating a slightly shorter red cell lifespan in the former group. The possible reasons for the difference between the two groups are discussed.", "contents": "Haemolysis after mitral valve replacement with the Bj\u00f6rk-Shiley and the Lillehei-Kaster disc valve prosthesis. The present study reports on the incidence and degree of intravascular haemolysis in 33 patients with Bj\u00f6rk-Shiley tilting disc prosthesis and in 34 patients with Lillehei-Kaster pivoting disc valve prosthesis in the mitral position examined 12 to 24 months after the operation. Serum haptoglobin, serum lactate dehydrogenase, serum bilirubin, and haemoglobin estimations were performed. Significant haemolysis was detected in 85 per cent of the patients. Haptoglobin was absent or reduced in 72 per cent, while raised values for serum lactate dehydrogenase were found in 43 per cent. The increase in lactate dehydrogenase was moderate and showed no correlation with blood flow through the prosthesis or with the gradient across the prosthesis. Most patients had normal haemoglobin and normal serum bilirubin values. Although intravascular haemolysis was of little clinical significance, the increase in lactate dehyrdrogenase was significantly higher in patients with the Lillehei-Kaster prosthesis than in patients with the Bj\u00f6rk Shiley prosthesis, indicating a slightly shorter red cell lifespan in the former group. The possible reasons for the difference between the two groups are discussed.", "PMID": 971382}
{"id": "pubmed23n0032_8460", "title": "Lack of effect of bed rest and cigarette smoking on development of deep venous thrombosis after myocardial infarction.", "content": "In a prospective study of patients admitted to a coronary care unit, the incidence of isotopically diagnosed deep venous thrombosis was found to be related to the severity of illness rather than to the duration of bed rest. In addition, no negative correlation was found between cigarette smoking and deep venous thrombosis.", "contents": "Lack of effect of bed rest and cigarette smoking on development of deep venous thrombosis after myocardial infarction. In a prospective study of patients admitted to a coronary care unit, the incidence of isotopically diagnosed deep venous thrombosis was found to be related to the severity of illness rather than to the duration of bed rest. In addition, no negative correlation was found between cigarette smoking and deep venous thrombosis.", "PMID": 971383}
{"id": "pubmed23n0032_8461", "title": "Transiet atrial fibrillation after minor head injury.", "content": "Two patients developed transient rapid atrial fibrillation after a blow on the head. There was no evidence of neurological damage or organic heart disease on subsequent investigation. Neither patient was aware of the cardiac irregularity.", "contents": "Transiet atrial fibrillation after minor head injury. Two patients developed transient rapid atrial fibrillation after a blow on the head. There was no evidence of neurological damage or organic heart disease on subsequent investigation. Neither patient was aware of the cardiac irregularity.", "PMID": 971384}
{"id": "pubmed23n0032_8462", "title": "Evaluation of rapid atrial pacing in diagnosis of coronary artery disease. Evaluation of atrial pacing test.", "content": "Seventeen patients presenting with anginal-type pain were studied by bicycle exercise testing, rapid atrial pacing, and coronary angiography. Ten patients with angina and abnormal pacing tests at rates less than 180/minute were found to have significant coronary artery disease as demonstrated by coronary angiography. Seven patients with pacing-induced chest pain only at rates of 180 and above had normal coronary angiogram. This suggests that patients requiring rates of 180 or more to produce a positive atrial pacing test, following our protocol, do not usually have significant coronary artery disease though confirmation requires a larger study.", "contents": "Evaluation of rapid atrial pacing in diagnosis of coronary artery disease. Evaluation of atrial pacing test. Seventeen patients presenting with anginal-type pain were studied by bicycle exercise testing, rapid atrial pacing, and coronary angiography. Ten patients with angina and abnormal pacing tests at rates less than 180/minute were found to have significant coronary artery disease as demonstrated by coronary angiography. Seven patients with pacing-induced chest pain only at rates of 180 and above had normal coronary angiogram. This suggests that patients requiring rates of 180 or more to produce a positive atrial pacing test, following our protocol, do not usually have significant coronary artery disease though confirmation requires a larger study.", "PMID": 971385}
{"id": "pubmed23n0032_8463", "title": "Giant blood cyst of tricuspid valve. Successful excision in an infant.", "content": "A case of a giant blood-filled cyst of the tricuspid valve in described in a 4-month-old infant. The cyst caused obstruction of the right ventricular inflow and outflow tracts and a right-to-left shunt; it was successfully removed at open heart surgery. Pitfalls in differential diagnosis and the pathogenesis are discussed.", "contents": "Giant blood cyst of tricuspid valve. Successful excision in an infant. A case of a giant blood-filled cyst of the tricuspid valve in described in a 4-month-old infant. The cyst caused obstruction of the right ventricular inflow and outflow tracts and a right-to-left shunt; it was successfully removed at open heart surgery. Pitfalls in differential diagnosis and the pathogenesis are discussed.", "PMID": 971386}
{"id": "pubmed23n0032_8464", "title": "Cervical aortic arch and a new type of double aortic arch. Report of a case.", "content": "A case of cervical aortic arch is reported. To the best of our knowledge, it is the first to be associated with a serious intracardiac anomaly. In addition, it is part of a new type of double aortic arch, caused by failure of reabsorption of both dorsal aortic roots and persistence of the fourth right and second (or third) left branchial arches.", "contents": "Cervical aortic arch and a new type of double aortic arch. Report of a case. A case of cervical aortic arch is reported. To the best of our knowledge, it is the first to be associated with a serious intracardiac anomaly. In addition, it is part of a new type of double aortic arch, caused by failure of reabsorption of both dorsal aortic roots and persistence of the fourth right and second (or third) left branchial arches.", "PMID": 971387}
{"id": "pubmed23n0032_8465", "title": "Plasma proteins, packed-cell volume and requirements for alcuronium.", "content": "The relationship between alcuronium requirements and the plasma concentration of albumin and total globulin was investigated in 50 adult patients undergoing elective upper abdominal surgery. No significant correlation was obtained with the plasma proteins (g/100 ml plasma). A significant positive correlation between alcuronium requirements and plasma albumin X (1-PCV), especially in anaemic patients, was found. This relationship tended to decrease with time.", "contents": "Plasma proteins, packed-cell volume and requirements for alcuronium. The relationship between alcuronium requirements and the plasma concentration of albumin and total globulin was investigated in 50 adult patients undergoing elective upper abdominal surgery. No significant correlation was obtained with the plasma proteins (g/100 ml plasma). A significant positive correlation between alcuronium requirements and plasma albumin X (1-PCV), especially in anaemic patients, was found. This relationship tended to decrease with time.", "PMID": 971391}
{"id": "pubmed23n0032_8466", "title": "Malignant hyperthermia in Belgian Landrace pigs rested or exercised before exposure to halothane.", "content": "Thirteen of 31 Belgian Landrace pigs developed malignant hyperthermia (MH) after breathing halothane. A short period of exercise 1 h before the administration of the triggering agent increased the incidence of the syndrome to 100% in eight similar pigs. Clinical symptoms were more marked and developed more rapidly in the exercised pigs. All the reacting pigs became typically acidotic, developed rigor and died. Serum Na+, K+, Ca2+, c.p.k., l.d.h. and protein concentrations were increased to a variable extent during the reaction and there was an increase in p.c.v. also. No hyperglycaemia was detected in pigs which were rested before receiving halothane. Four of the eight exercised pigs became markedly hyperglycaemic and plasma noradrenaline increased to higher values. Phosphocreatine and ATP decreased to low values and lactate increased in the muscles of all pigs which reacted. At the time of death, muscle glycogen had decreased significantly in the rested, but not in the exercised, MH pigs.", "contents": "Malignant hyperthermia in Belgian Landrace pigs rested or exercised before exposure to halothane. Thirteen of 31 Belgian Landrace pigs developed malignant hyperthermia (MH) after breathing halothane. A short period of exercise 1 h before the administration of the triggering agent increased the incidence of the syndrome to 100% in eight similar pigs. Clinical symptoms were more marked and developed more rapidly in the exercised pigs. All the reacting pigs became typically acidotic, developed rigor and died. Serum Na+, K+, Ca2+, c.p.k., l.d.h. and protein concentrations were increased to a variable extent during the reaction and there was an increase in p.c.v. also. No hyperglycaemia was detected in pigs which were rested before receiving halothane. Four of the eight exercised pigs became markedly hyperglycaemic and plasma noradrenaline increased to higher values. Phosphocreatine and ATP decreased to low values and lactate increased in the muscles of all pigs which reacted. At the time of death, muscle glycogen had decreased significantly in the rested, but not in the exercised, MH pigs.", "PMID": 971390}
{"id": "pubmed23n0032_8467", "title": "Alveolar gas relationships during the use of semi-closed rebreathing anaesthetic systems.", "content": "A black box and a geometrical approach have been used to deduce the relationships between alveolar oxygen and carbon dioxide concentrations when rebreathing occurs during the use of semi-closed anaesthetic rebreathing systems.", "contents": "Alveolar gas relationships during the use of semi-closed rebreathing anaesthetic systems. A black box and a geometrical approach have been used to deduce the relationships between alveolar oxygen and carbon dioxide concentrations when rebreathing occurs during the use of semi-closed anaesthetic rebreathing systems.", "PMID": 971392}
{"id": "pubmed23n0032_8468", "title": "The hydrolysis of suxamethonium in human blood.", "content": "In 30 patients, after induction of anaesthesia, the ulnar nerve on both arms was stimulated, and the resulting had contractions recorded or closely observed. Immediately after the circulation to one arm had been occluded with a tourniquet, suxamethonium 1 mg/kg was injected i.v. The occlusion was relieved after 1, 2 or 3 min. It was found that the duration of block on the occluded arm represented 68, 48 and 19% of the non-occluded arm after 1, 2 and 3 min occlusion respectively. Thus a considerable recirculation of the drug occurs, even 3 min after injection. It can be calculated that the disappearance of suxamethonium from blood is 7.7 mg/litre in the 1st min and 2.9 mg/litre in both the 2nd and the 3rd min. These disappearance rates include both hydrolysis and diffusion from plasma. Thus the rate for hydrolysis contrasts sharply with the published values for suxamethonium hydrolysis in vitro.", "contents": "The hydrolysis of suxamethonium in human blood. In 30 patients, after induction of anaesthesia, the ulnar nerve on both arms was stimulated, and the resulting had contractions recorded or closely observed. Immediately after the circulation to one arm had been occluded with a tourniquet, suxamethonium 1 mg/kg was injected i.v. The occlusion was relieved after 1, 2 or 3 min. It was found that the duration of block on the occluded arm represented 68, 48 and 19% of the non-occluded arm after 1, 2 and 3 min occlusion respectively. Thus a considerable recirculation of the drug occurs, even 3 min after injection. It can be calculated that the disappearance of suxamethonium from blood is 7.7 mg/litre in the 1st min and 2.9 mg/litre in both the 2nd and the 3rd min. These disappearance rates include both hydrolysis and diffusion from plasma. Thus the rate for hydrolysis contrasts sharply with the published values for suxamethonium hydrolysis in vitro.", "PMID": 971393}
{"id": "pubmed23n0032_8469", "title": "Plasma lignocaine concentrations following endotracheal spraying with an aerosol.", "content": "Plasma concentrations of lignocaine were measured in three groups of anaesthetized patients following spraying of the trachea and larynx with a lignocaine 10% aerosol spray. Greater venous plasma concentrations occurred in patients who were paralysed with suxamethonium. A mean plasma concentration of 0.1 mug/ml of lignocaine resulted from each 10 mg of lignocaine used in spontaneously breathing patients, and 0.15 mug/ml in paralysed patients. In individual patients a concentration 50% in excess of the mean value may occur. The use of lignocaine 100 mg as a 10% aerosol spray can be considered safe.", "contents": "Plasma lignocaine concentrations following endotracheal spraying with an aerosol. Plasma concentrations of lignocaine were measured in three groups of anaesthetized patients following spraying of the trachea and larynx with a lignocaine 10% aerosol spray. Greater venous plasma concentrations occurred in patients who were paralysed with suxamethonium. A mean plasma concentration of 0.1 mug/ml of lignocaine resulted from each 10 mg of lignocaine used in spontaneously breathing patients, and 0.15 mug/ml in paralysed patients. In individual patients a concentration 50% in excess of the mean value may occur. The use of lignocaine 100 mg as a 10% aerosol spray can be considered safe.", "PMID": 971394}
{"id": "pubmed23n0032_8470", "title": "Impairment of blood fibrinolytic activity during major surgical stress under combined extradural blockade and general anaesthesia.", "content": "Blood fibrinolytic activity and plasma cortisol concentrations were investigated in 15 patients undergoing upper or lower abdominal surgery under high or low extradural blockade plus general anesthesia. It was found that the initial enhancement of fibrinolysis could be prevented but only in those patients showing impaired cortisol responses. This suggests a common nervous mechanism unrelated to the degree of sensory blockade.", "contents": "Impairment of blood fibrinolytic activity during major surgical stress under combined extradural blockade and general anaesthesia. Blood fibrinolytic activity and plasma cortisol concentrations were investigated in 15 patients undergoing upper or lower abdominal surgery under high or low extradural blockade plus general anesthesia. It was found that the initial enhancement of fibrinolysis could be prevented but only in those patients showing impaired cortisol responses. This suggests a common nervous mechanism unrelated to the degree of sensory blockade.", "PMID": 971395}
{"id": "pubmed23n0032_8471", "title": "The pulmonary capillary bed after lymphography.", "content": "A small series of patients underwent radio-isotope lung scanning before and after the bipedal injection of oily contrast medium for abdominal lymphography to assess known malignant disease. After lymphography the lung scans showed an increase of between 12 and 100% in the radioactivity recorded over the anterior chest. No change was recorded over the posterior aspect of the chest. It is suggested that the increase is a result of blockage of posteriorly placed pulmonary capillaries and diversion of blood flow to the anterior segments.", "contents": "The pulmonary capillary bed after lymphography. A small series of patients underwent radio-isotope lung scanning before and after the bipedal injection of oily contrast medium for abdominal lymphography to assess known malignant disease. After lymphography the lung scans showed an increase of between 12 and 100% in the radioactivity recorded over the anterior chest. No change was recorded over the posterior aspect of the chest. It is suggested that the increase is a result of blockage of posteriorly placed pulmonary capillaries and diversion of blood flow to the anterior segments.", "PMID": 971396}
{"id": "pubmed23n0032_8472", "title": "A proinflammatory factor in lymphocytes. Its role in the development of acute, non-immunological inflammatory reactions.", "content": "Drug-induced leucopenia renders rats hyporeactive to various inflammatory stimuli. Administration to leucopenic rats of suspensions of lymphocytes, sufficient to apparently correct the induced lymphocytopenia, led to a partial but marked reversal of the inhibited responses. Similar results were observed when lysates of lymphocytes or filtrates of the disintegrated cells were injected. Suspensions of polymorphonuclear granulocytes, on the contrary, were ineffective in producing a reversal of inhibited inflammatory reactions in leucopenic rats. The presence of a proinflammatory factor (LpIF) in lymphocytes, which might be involved in the modulation of acute inflammatory responses is suggested.", "contents": "A proinflammatory factor in lymphocytes. Its role in the development of acute, non-immunological inflammatory reactions. Drug-induced leucopenia renders rats hyporeactive to various inflammatory stimuli. Administration to leucopenic rats of suspensions of lymphocytes, sufficient to apparently correct the induced lymphocytopenia, led to a partial but marked reversal of the inhibited responses. Similar results were observed when lysates of lymphocytes or filtrates of the disintegrated cells were injected. Suspensions of polymorphonuclear granulocytes, on the contrary, were ineffective in producing a reversal of inhibited inflammatory reactions in leucopenic rats. The presence of a proinflammatory factor (LpIF) in lymphocytes, which might be involved in the modulation of acute inflammatory responses is suggested.", "PMID": 971405}
{"id": "pubmed23n0032_8473", "title": "The effect of furosemide on the renal damage induced by toxic mushroom Cortinarius speciosissimus in the rat.", "content": "The effect of furosemide on the renal damage induced by the toxic mushroom Cortinarius speciosissimus was studied in female rats. Furosemide (50 mg/kg s.c.) was given 15 min before the mushroom administration (250 mg dried mushroom/kg body weight p.o.). It was observed that furosemide clearly increased the tubular damage induced by C. speciosissimus. In contrast, furosemide had no effect on the inflammation induced by this toxic mushroom.", "contents": "The effect of furosemide on the renal damage induced by toxic mushroom Cortinarius speciosissimus in the rat. The effect of furosemide on the renal damage induced by the toxic mushroom Cortinarius speciosissimus was studied in female rats. Furosemide (50 mg/kg s.c.) was given 15 min before the mushroom administration (250 mg dried mushroom/kg body weight p.o.). It was observed that furosemide clearly increased the tubular damage induced by C. speciosissimus. In contrast, furosemide had no effect on the inflammation induced by this toxic mushroom.", "PMID": 971406}
{"id": "pubmed23n0032_8474", "title": "Effects of isoprenaline and pilocarpine on salivary glands as assessed by gravimetric and image analysis techniques.", "content": "Pigs were given 6 daily injections of isoprenaline or pilocarpine. At necropsy the parotid, submaxillary and sublingual salivary glands were removed and weighed. Histological sections were prepared from several regions of each gland and the cross-sectional area of their acini was measured in treated and control animals by means of a Quantimet Image Analysis System. Both isoprenaline and pilocarpine produced a significant increase in the weight of the parotid and submaxillary glands, which was accompanied by an increase in acinar area. There was no change in the weight of the sublingual gland with either drug, but isoprenaline did induce an increase in the area of sublingual acini. A comparison is made of the changes in weight and structure of the salivary glands as shown by these methods.", "contents": "Effects of isoprenaline and pilocarpine on salivary glands as assessed by gravimetric and image analysis techniques. Pigs were given 6 daily injections of isoprenaline or pilocarpine. At necropsy the parotid, submaxillary and sublingual salivary glands were removed and weighed. Histological sections were prepared from several regions of each gland and the cross-sectional area of their acini was measured in treated and control animals by means of a Quantimet Image Analysis System. Both isoprenaline and pilocarpine produced a significant increase in the weight of the parotid and submaxillary glands, which was accompanied by an increase in acinar area. There was no change in the weight of the sublingual gland with either drug, but isoprenaline did induce an increase in the area of sublingual acini. A comparison is made of the changes in weight and structure of the salivary glands as shown by these methods.", "PMID": 971407}
{"id": "pubmed23n0032_8475", "title": "Labelled polyvinylpyrrolidone as an in vivo indicator of reticuloendothelial activity.", "content": "It is shown that after a single i.v. dose of [131I]-polyvinylpyrrolidone ([131I]-PVP) the total body and plasma radioactivities of rabbits decrease at distinctly different rates. The difference between these two rates is utilized to calculate the phagocytic rate of [131I]-PVP by reticuloendothelial cells.A number of experimental conditions are reported in which enhanced reticuloendothelial uptake of [131I]-PVP is readily demonstrable. They include the injection of small quantities of heterologous plasma, certain proteolytic fragments of the fibrinogen molecule, the clearance of antigen-antibody complexes, and the acute phase reaction (inflammatory response) as brought about by serum sickness, sterile abscess and vaccination. Based on these observations it is suggested that [131I]-PVP may provide a convenient technique for the long-term monitoring of the activity of reticuloendothelial cells, presumably mainly that of the histiocytes. The pronounced polydispersity of commercially available [131I]-PVP is a serious problem in this respect which can be largely overcome, but not completely abolished, by the screening techniques described herein. Post-mortem analyses of rabbit tissues showed most of the [131I]-PVP to be present in the skin (20%), followed by the liver (14%), bone marrow (10%), muscle (7%) and kidney (5%). Gel filtration studies with [131]-PVP in the presence and in the absence of plasma proteins failed to demonstrate any association between PVP and the proteins. [131I]-PVP kept at physiological pH and 37 degrees C lost less than 5% of its radioactivity over one month due to spontaneous deiodination.", "contents": "Labelled polyvinylpyrrolidone as an in vivo indicator of reticuloendothelial activity. It is shown that after a single i.v. dose of [131I]-polyvinylpyrrolidone ([131I]-PVP) the total body and plasma radioactivities of rabbits decrease at distinctly different rates. The difference between these two rates is utilized to calculate the phagocytic rate of [131I]-PVP by reticuloendothelial cells.A number of experimental conditions are reported in which enhanced reticuloendothelial uptake of [131I]-PVP is readily demonstrable. They include the injection of small quantities of heterologous plasma, certain proteolytic fragments of the fibrinogen molecule, the clearance of antigen-antibody complexes, and the acute phase reaction (inflammatory response) as brought about by serum sickness, sterile abscess and vaccination. Based on these observations it is suggested that [131I]-PVP may provide a convenient technique for the long-term monitoring of the activity of reticuloendothelial cells, presumably mainly that of the histiocytes. The pronounced polydispersity of commercially available [131I]-PVP is a serious problem in this respect which can be largely overcome, but not completely abolished, by the screening techniques described herein. Post-mortem analyses of rabbit tissues showed most of the [131I]-PVP to be present in the skin (20%), followed by the liver (14%), bone marrow (10%), muscle (7%) and kidney (5%). Gel filtration studies with [131]-PVP in the presence and in the absence of plasma proteins failed to demonstrate any association between PVP and the proteins. [131I]-PVP kept at physiological pH and 37 degrees C lost less than 5% of its radioactivity over one month due to spontaneous deiodination.", "PMID": 971408}
{"id": "pubmed23n0032_8476", "title": "Toxicity of various carrageenans in the mouse.", "content": "Carrageenan toxicity was found to vary according to the biochemical nature and source of material injected. Ischaemic lesions of body extremities (acronecrosis) were produced by some but not all preparations. Histological examination of these sites and of both recent and old organ lesions (especially of liver and kidney) confirmed that the underlying pathology was disseminated intravascular coagulation. Evidence was also obtained that carrageenans are hepatotoxic.", "contents": "Toxicity of various carrageenans in the mouse. Carrageenan toxicity was found to vary according to the biochemical nature and source of material injected. Ischaemic lesions of body extremities (acronecrosis) were produced by some but not all preparations. Histological examination of these sites and of both recent and old organ lesions (especially of liver and kidney) confirmed that the underlying pathology was disseminated intravascular coagulation. Evidence was also obtained that carrageenans are hepatotoxic.", "PMID": 971409}
{"id": "pubmed23n0032_8477", "title": "Mouse hydrocephalus and encephalitis following intracerebral infection with Newcastle disease vaccine viruses.", "content": "Hydrocephalus and encephalitis in 14-day-old mice was induced by an intracerebral inoculation of a high dose of live Newcastle disease vaccine viruses. The distended lateral ventricles occurred as a result of aqueduct stenosis superimposed on ciliary degeneration and destruction of ependymal cells. The brain virus titres were high on Days 3, 5 and 7 but never exceeded the titre of the original dose given. Positive serum haemagglutination-inhibition titres persisted throughout the experiment.", "contents": "Mouse hydrocephalus and encephalitis following intracerebral infection with Newcastle disease vaccine viruses. Hydrocephalus and encephalitis in 14-day-old mice was induced by an intracerebral inoculation of a high dose of live Newcastle disease vaccine viruses. The distended lateral ventricles occurred as a result of aqueduct stenosis superimposed on ciliary degeneration and destruction of ependymal cells. The brain virus titres were high on Days 3, 5 and 7 but never exceeded the titre of the original dose given. Positive serum haemagglutination-inhibition titres persisted throughout the experiment.", "PMID": 971410}
{"id": "pubmed23n0032_8478", "title": "Metabolism of biliverdin. Biliary excretion of bile pigments after intravenous injection of biliverdin isomers.", "content": "14C-labelled biliverdins IX alpha, beta, gamma and delta have been prepared in vitro from haemoglobin obtained from duck erythrocytes incubated with 5-amino[4-14C]-laevulinic acid. When injected intravenously into rats with biliary fistulae, about 60% of the label was recovered in the bile in 24 h after the alpha isomer was given, while approximately 10% was recovered with injection of the beta isomer. The gamma and delta isomers gave intermediate values. In each experiment, most of the recovered isotope was found in association with conjugated bile pigment. Thus, the metabolic pathway for bile pigment excretion in the rat handles the IX alpha isomer preferentially but is not specific for it.", "contents": "Metabolism of biliverdin. Biliary excretion of bile pigments after intravenous injection of biliverdin isomers. 14C-labelled biliverdins IX alpha, beta, gamma and delta have been prepared in vitro from haemoglobin obtained from duck erythrocytes incubated with 5-amino[4-14C]-laevulinic acid. When injected intravenously into rats with biliary fistulae, about 60% of the label was recovered in the bile in 24 h after the alpha isomer was given, while approximately 10% was recovered with injection of the beta isomer. The gamma and delta isomers gave intermediate values. In each experiment, most of the recovered isotope was found in association with conjugated bile pigment. Thus, the metabolic pathway for bile pigment excretion in the rat handles the IX alpha isomer preferentially but is not specific for it.", "PMID": 971411}
{"id": "pubmed23n0032_8479", "title": "Structure of the three major sialyl-oligosaccharides excreted in the urine of five patients with three distinct inborn diseases: \"I cell disease\" and two new types of mucolipidosis.", "content": "The urine of five patients with three distinct diseases (\"I Cell disease\" and two new types of mucolipidosis) contains sialic acid-rich oligosaccharides in a high amount: 50- to 500-fold the normal. The structure of the major components are as follows: alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNac(1 leads to 2)alphaMan(1 leads to 3)betaMan(1 leads to 4)GlcNac,[alphaAcNeu(2 leads to 6)]betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 3)[betaGal(1 leads to 4)betaGlcNac(1 leads to 2)alphaMan(1 leads to 6)]betaMan(1 leads to 4)GlcNAc and alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 3)[alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 6)]betaMan(1 leads to 4)GlcNAc. These results suggest that a deficit in alpha-neuraminidase is associated to these three different disorders and that an endo-beta-D-N-acetylglucosaminidase is able to release sialyoligosaccharides by splitting the sialylglycans of glycoproteins.", "contents": "Structure of the three major sialyl-oligosaccharides excreted in the urine of five patients with three distinct inborn diseases: \"I cell disease\" and two new types of mucolipidosis. The urine of five patients with three distinct diseases (\"I Cell disease\" and two new types of mucolipidosis) contains sialic acid-rich oligosaccharides in a high amount: 50- to 500-fold the normal. The structure of the major components are as follows: alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNac(1 leads to 2)alphaMan(1 leads to 3)betaMan(1 leads to 4)GlcNac,[alphaAcNeu(2 leads to 6)]betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 3)[betaGal(1 leads to 4)betaGlcNac(1 leads to 2)alphaMan(1 leads to 6)]betaMan(1 leads to 4)GlcNAc and alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 3)[alphaAcNeu(2 leads to 6)betaGal(1 leads to 4)betaGlcNAc(1 leads to 2)alphaMan(1 leads to 6)]betaMan(1 leads to 4)GlcNAc. These results suggest that a deficit in alpha-neuraminidase is associated to these three different disorders and that an endo-beta-D-N-acetylglucosaminidase is able to release sialyoligosaccharides by splitting the sialylglycans of glycoproteins.", "PMID": 971412}
{"id": "pubmed23n0032_8480", "title": "Effect of mucolytic agents on the rheological properties of tracheal mucus.", "content": "Canine tracheal mucus was dissolved by a number of mucolytic agents, including disulfide bond reducing agents, hydrogen bond breaking agents, and chaotropic ions, and their effect on rheological properties was assessed. Sodium thiocyanate led to 85-100% dissolution with the maximum retention of elasticity. Thiocyanate exposure did not result in demonstrable alterations in the size or shape of the mucus glycoproteins. Sodium thiocyanate is therefore recommended as a suitable dispersing agent for physiochemical studies of glycoprotein secretions.", "contents": "Effect of mucolytic agents on the rheological properties of tracheal mucus. Canine tracheal mucus was dissolved by a number of mucolytic agents, including disulfide bond reducing agents, hydrogen bond breaking agents, and chaotropic ions, and their effect on rheological properties was assessed. Sodium thiocyanate led to 85-100% dissolution with the maximum retention of elasticity. Thiocyanate exposure did not result in demonstrable alterations in the size or shape of the mucus glycoproteins. Sodium thiocyanate is therefore recommended as a suitable dispersing agent for physiochemical studies of glycoprotein secretions.", "PMID": 971413}
{"id": "pubmed23n0032_8481", "title": "Copper deficiency and erythrocuprein (2Cu, 2Zn-superoxide dismutase).", "content": "The activities of 2Cu,2Zn-superoxide dismutase, ferroxidase (ceruloplasmin), catalase and glutathione peroxidase were measured in the blood of rats during copper depletion. Two control groups of animals were used; one received the regular diet containing all essential components including copper and the other group was maintained on a diet, containing 1% the amount of copper in normal diet, copper being supplied as Cu(Leu)2 in the drinking water. Both groups showed no detectable differences, either in the copper content of blood or in the  measured four enzymic activities. Excessive copper (injected intraperitoneally) caused only an insignificant rise in the enzymic activities (0-10%) compared to either control. After starting copper depletion ferroxidase activity decreases to 15% on the 15th day, while the 2Cu,2Zn-superoxide dismutase activity decreases to 40% on the 45th day. Ferroxidase activity shows rapid but transient changes immediately after perturbation in plasma copper levels. By contrast, the 2Cu,2Zn-superoxide dismutase activity more closely parallels the overall copper deficiency. Dietary repletion with copper raises the 2Cu,2Zn-superoxide dismutase activity to 94% and the ferroxidase activity to 80% of the control values within 36 h. Apart from the copper-dependent anemia catalase activity was decreased. However, 15 days after the start of the copper depletion catalase activity rises again and reaches the control value on the 40th day and a 30% stimulation was even seen on the 58th day. Upon copper repletion catalase activity reaches 166% of the control within 14 days. No copper-dependent differences of glutathione peroxidase activity were seen regardless whatever copper level was present in the rats.", "contents": "Copper deficiency and erythrocuprein (2Cu, 2Zn-superoxide dismutase). The activities of 2Cu,2Zn-superoxide dismutase, ferroxidase (ceruloplasmin), catalase and glutathione peroxidase were measured in the blood of rats during copper depletion. Two control groups of animals were used; one received the regular diet containing all essential components including copper and the other group was maintained on a diet, containing 1% the amount of copper in normal diet, copper being supplied as Cu(Leu)2 in the drinking water. Both groups showed no detectable differences, either in the copper content of blood or in the  measured four enzymic activities. Excessive copper (injected intraperitoneally) caused only an insignificant rise in the enzymic activities (0-10%) compared to either control. After starting copper depletion ferroxidase activity decreases to 15% on the 15th day, while the 2Cu,2Zn-superoxide dismutase activity decreases to 40% on the 45th day. Ferroxidase activity shows rapid but transient changes immediately after perturbation in plasma copper levels. By contrast, the 2Cu,2Zn-superoxide dismutase activity more closely parallels the overall copper deficiency. Dietary repletion with copper raises the 2Cu,2Zn-superoxide dismutase activity to 94% and the ferroxidase activity to 80% of the control values within 36 h. Apart from the copper-dependent anemia catalase activity was decreased. However, 15 days after the start of the copper depletion catalase activity rises again and reaches the control value on the 40th day and a 30% stimulation was even seen on the 58th day. Upon copper repletion catalase activity reaches 166% of the control within 14 days. No copper-dependent differences of glutathione peroxidase activity were seen regardless whatever copper level was present in the rats.", "PMID": 971414}
{"id": "pubmed23n0032_8482", "title": "The stability of porphyrin and metalloporphyrin molecular complexes in solution.", "content": "Stability constants for the molecular complexes formed by protoporphyrin IX dimethyl ester and its metal chelates with Co2+, Ni2+, Cu2+, Zn2+, Mg2+ and Fe3+ with nitroarene acceptors are determined in non-aqueous solvents. The stability of the complexes is found to be largely insensitive to variations in the central metal ions provided they are in the same oxidation state. Solvent-solute interactions are seen as being important in the formation of such complexes giving rise to large variations in their apparent stability. Some of the other factors that affect the stability of the molecular complexes are also evaluated. No evidence has been found for the appearance of any intermolecular charge-transfer absorption bands. Infrared studies, however, have provided some evidence for a substantial loss of electron density from the porphyrin ring upon interactions with the acceptors.", "contents": "The stability of porphyrin and metalloporphyrin molecular complexes in solution. Stability constants for the molecular complexes formed by protoporphyrin IX dimethyl ester and its metal chelates with Co2+, Ni2+, Cu2+, Zn2+, Mg2+ and Fe3+ with nitroarene acceptors are determined in non-aqueous solvents. The stability of the complexes is found to be largely insensitive to variations in the central metal ions provided they are in the same oxidation state. Solvent-solute interactions are seen as being important in the formation of such complexes giving rise to large variations in their apparent stability. Some of the other factors that affect the stability of the molecular complexes are also evaluated. No evidence has been found for the appearance of any intermolecular charge-transfer absorption bands. Infrared studies, however, have provided some evidence for a substantial loss of electron density from the porphyrin ring upon interactions with the acceptors.", "PMID": 971415}
{"id": "pubmed23n0032_8483", "title": "Transfer of heme from heme-albumin to hemopexin.", "content": "Exchange of heme in vitro between two heme-binding serum proteins, albumin and hemopexin, was examined spectrophotometrically. Hemopexin, albumin and heme in molar ratios of 1 : 70 : 1 were incubated at 22 degrees C, pH 7.3. The heme was added as free heme, heme-hemopexin or methemalbumin. Due to the high affinity of hemopexin for heme, Kd near 10(-13) M, only negligible amounts of heme were transferred from hemopexin to albumin in 48 h. However, more than 80% of heme was transferred from methemalbumin to hemopexin within 24 h. Heme added to a 1 : 70 mixture of the apo-proteins is initially bound by albumin; but more than 90% is bound by hemopexin in 24 h. Addition of dithionite causes nearly all of the heme present, whether added as free heme or methemalbumin, to associate with hemopexin in 15 min. Albumin thus appears to have a much lower affinity for ferro- than for ferri-heme. Results obtained from similar experiments with human serum and human serum made hemopexin-free by immunoadsorption fully corroborate those obtained with mixtures of purified albumin and hemopexin. These observations suggest that the rate-limiting step in the heme transport function of hemopexin is the formation of the heme-hemopexin complex, rather than the uptake of the complex by the liver.", "contents": "Transfer of heme from heme-albumin to hemopexin. Exchange of heme in vitro between two heme-binding serum proteins, albumin and hemopexin, was examined spectrophotometrically. Hemopexin, albumin and heme in molar ratios of 1 : 70 : 1 were incubated at 22 degrees C, pH 7.3. The heme was added as free heme, heme-hemopexin or methemalbumin. Due to the high affinity of hemopexin for heme, Kd near 10(-13) M, only negligible amounts of heme were transferred from hemopexin to albumin in 48 h. However, more than 80% of heme was transferred from methemalbumin to hemopexin within 24 h. Heme added to a 1 : 70 mixture of the apo-proteins is initially bound by albumin; but more than 90% is bound by hemopexin in 24 h. Addition of dithionite causes nearly all of the heme present, whether added as free heme or methemalbumin, to associate with hemopexin in 15 min. Albumin thus appears to have a much lower affinity for ferro- than for ferri-heme. Results obtained from similar experiments with human serum and human serum made hemopexin-free by immunoadsorption fully corroborate those obtained with mixtures of purified albumin and hemopexin. These observations suggest that the rate-limiting step in the heme transport function of hemopexin is the formation of the heme-hemopexin complex, rather than the uptake of the complex by the liver.", "PMID": 971416}
{"id": "pubmed23n0032_8484", "title": "Influence of tryptophan on the level of hepatic microsomal cytochrome P-450 in well-fed normal, adrenalectomized, and phenobarbital-treated rats.", "content": "In well-fed normal male rats, either force-feeding of tryptophan or a single injection of phenobarbital produced significant increments in hepatic microsomal cytochrome P-450 and the associated aniline hydroxylase activity. Administration of both tryptophan and phenobarbital together resulted in even greater stimulation than when the compounds were given alone. Adrenalectomy lowered instead the cytochrome P-450 concentration in comparison with that of normal rats, and administration of tryptophan and phenobarbital in this condition produced no significant increment in cytochrome P-450 concentration. In addition, phenobarbital administered either singly or in combination with tryptophan resulted in 80% mortality, which was reduced to zero by pretreatment with cortisol. While in cortisol-treated adrenalectomized rats administration of phenobarbital caused a 56% increment in cytochrome P-450 as compared to controls, tryptophan produced only a minor (9%) increase. In normal, as well as in adrenalectomized rats, tryptophan and phenobarbital administered either idividually or together increased microsomal protein concentration. In normal rats actinomycin-D treatment reduced both cytochrome P-450 and microsomal protein concentrations below that of the non-treated control levels. Further administration of either tryptophan or phenobarbital slightly increased the level of cytochrome P-450, and the two compounds together caused 40 and 21% increments of the same compared to actinomycin-treated and non-treated controls, respectively.", "contents": "Influence of tryptophan on the level of hepatic microsomal cytochrome P-450 in well-fed normal, adrenalectomized, and phenobarbital-treated rats. In well-fed normal male rats, either force-feeding of tryptophan or a single injection of phenobarbital produced significant increments in hepatic microsomal cytochrome P-450 and the associated aniline hydroxylase activity. Administration of both tryptophan and phenobarbital together resulted in even greater stimulation than when the compounds were given alone. Adrenalectomy lowered instead the cytochrome P-450 concentration in comparison with that of normal rats, and administration of tryptophan and phenobarbital in this condition produced no significant increment in cytochrome P-450 concentration. In addition, phenobarbital administered either singly or in combination with tryptophan resulted in 80% mortality, which was reduced to zero by pretreatment with cortisol. While in cortisol-treated adrenalectomized rats administration of phenobarbital caused a 56% increment in cytochrome P-450 as compared to controls, tryptophan produced only a minor (9%) increase. In normal, as well as in adrenalectomized rats, tryptophan and phenobarbital administered either idividually or together increased microsomal protein concentration. In normal rats actinomycin-D treatment reduced both cytochrome P-450 and microsomal protein concentrations below that of the non-treated control levels. Further administration of either tryptophan or phenobarbital slightly increased the level of cytochrome P-450, and the two compounds together caused 40 and 21% increments of the same compared to actinomycin-treated and non-treated controls, respectively.", "PMID": 971417}
{"id": "pubmed23n0032_8485", "title": "Multiple glucocorticoid binding components of intact AtT-20/D-1 mouse pituitary tumor cells.", "content": "Glucocorticoids were found to bind to two components in the AtT-20/D-1 pituitary tumor cell. One component was characterized by slow dissociation of the bound steroid, stringent glucocorticoid specificity and high steroid binding affinity (Kd = 4.64 - 10(-9) M for triamcinolone acetonide). Thus, the characteristics of this component, termed the slowly dissociable component, resembled those of the soluble cytosol receptor. The other component exhbited lower binding affinity (Kd = 1.57 - 10(-7) M for triamcinolone acetonide), less stringent glucocorticoid specificity, and very rapid dissociation of bound, labelled glucocorticoid, Binding to this component, termed the rapidly dissociable component, represented 60% of total steroid binding to intact cells at 4 degrees C. Incubation of intact cells at 25 degrees C caused a progressive increase in steroid binding to the slowly dissociable component with no change in the absolute amount of ste roidal binding to the rapidly dissociable component. The high-binding affinity and preference for glucocorticoids shown by both components favor their function as biologically significant mediators of steroid action in this glucocorticoid responsive cell.", "contents": "Multiple glucocorticoid binding components of intact AtT-20/D-1 mouse pituitary tumor cells. Glucocorticoids were found to bind to two components in the AtT-20/D-1 pituitary tumor cell. One component was characterized by slow dissociation of the bound steroid, stringent glucocorticoid specificity and high steroid binding affinity (Kd = 4.64 - 10(-9) M for triamcinolone acetonide). Thus, the characteristics of this component, termed the slowly dissociable component, resembled those of the soluble cytosol receptor. The other component exhbited lower binding affinity (Kd = 1.57 - 10(-7) M for triamcinolone acetonide), less stringent glucocorticoid specificity, and very rapid dissociation of bound, labelled glucocorticoid, Binding to this component, termed the rapidly dissociable component, represented 60% of total steroid binding to intact cells at 4 degrees C. Incubation of intact cells at 25 degrees C caused a progressive increase in steroid binding to the slowly dissociable component with no change in the absolute amount of ste roidal binding to the rapidly dissociable component. The high-binding affinity and preference for glucocorticoids shown by both components favor their function as biologically significant mediators of steroid action in this glucocorticoid responsive cell.", "PMID": 971418}
{"id": "pubmed23n0032_8486", "title": "Heterogeneity of pituitary glucocorticoid binding evidence for a transcortin-like compound.", "content": "Scatchard analysis, as well as sucrose gradient centrifugation demonstrate that at least two classes of specific glucocorticoid binding sites occur in the adenohypophysis. One, exhibited high affinity for both corticosterone and dexamethasone (receptor D), while the other bound only corticosterone. The latter binder revealed physico-chemical properties closely similar to those of plasma transcortin, but was shown not to be due to simple blood contamination of cytosol. This transcortin-like (TL) component, however, seemed to be carried over from plasma, since the concentration of pituitary binding sites strikingly paralleled physiological variations of plasma transcortin. Furthermore, experiments carried out with isolated pituitary cells suggested an intracellular location of that binder, although sticking to the cell membrane cannot be excluded. Finally, it was shown that the TL compound was unable, unlike the receptor D, to transfer glucocorticoids into the nuclei. One of its possible roles could be to modulate the interaction between steroids and compound D.", "contents": "Heterogeneity of pituitary glucocorticoid binding evidence for a transcortin-like compound. Scatchard analysis, as well as sucrose gradient centrifugation demonstrate that at least two classes of specific glucocorticoid binding sites occur in the adenohypophysis. One, exhibited high affinity for both corticosterone and dexamethasone (receptor D), while the other bound only corticosterone. The latter binder revealed physico-chemical properties closely similar to those of plasma transcortin, but was shown not to be due to simple blood contamination of cytosol. This transcortin-like (TL) component, however, seemed to be carried over from plasma, since the concentration of pituitary binding sites strikingly paralleled physiological variations of plasma transcortin. Furthermore, experiments carried out with isolated pituitary cells suggested an intracellular location of that binder, although sticking to the cell membrane cannot be excluded. Finally, it was shown that the TL compound was unable, unlike the receptor D, to transfer glucocorticoids into the nuclei. One of its possible roles could be to modulate the interaction between steroids and compound D.", "PMID": 971419}
{"id": "pubmed23n0032_8487", "title": "Establishment of the integrity of lysosomes in a glycoprotein-rich matrix. Distribution pattern of seven lysosomal enzymes in gastric mucosa.", "content": "Gel-forming mucosal glycoproteins strongly interfere with standard methods of cell fractionation. Thus, acid hydrolase-bound particles imbedded in the gel, sediment on centrifugation, in the nuclear fraction of homogenates of canine antral mucosa. These particles can be cleared by direct solubilization of the gel; however, the viscosity of the solution obtained prevents sedimentation of some of the latent hydrolases, even at very high speeds. The use of a new step-wise scheme of centrifugation and dilution successfully isolates lysosomal particles containing acid hydrolases from mucin-rich mucosa. All of the enzymes investigated, including acid phosphatase, cathepsin D, alpha- and beta-galactosidase, beta-B-acetylhexosaminidases, but with the exception of alpha-fucosidase, were found to be particle bound, exhibiting high degrees of latency. However, active mucosal particles are polydisperase in size and density, sedimenting under different centrifugal forces.", "contents": "Establishment of the integrity of lysosomes in a glycoprotein-rich matrix. Distribution pattern of seven lysosomal enzymes in gastric mucosa. Gel-forming mucosal glycoproteins strongly interfere with standard methods of cell fractionation. Thus, acid hydrolase-bound particles imbedded in the gel, sediment on centrifugation, in the nuclear fraction of homogenates of canine antral mucosa. These particles can be cleared by direct solubilization of the gel; however, the viscosity of the solution obtained prevents sedimentation of some of the latent hydrolases, even at very high speeds. The use of a new step-wise scheme of centrifugation and dilution successfully isolates lysosomal particles containing acid hydrolases from mucin-rich mucosa. All of the enzymes investigated, including acid phosphatase, cathepsin D, alpha- and beta-galactosidase, beta-B-acetylhexosaminidases, but with the exception of alpha-fucosidase, were found to be particle bound, exhibiting high degrees of latency. However, active mucosal particles are polydisperase in size and density, sedimenting under different centrifugal forces.", "PMID": 971420}
{"id": "pubmed23n0032_8488", "title": "Reduction by cycloheximide of lysosomal proteolytic enzyme activity and rate of protein degradation in organ-cultured hearts.", "content": "Cycloheximide, an agent whose primary action is inhibition of protein synthesis, also causes a decrease in the rate of protein degradation in cultured fetal mouse hearts. This is associated with marked decreases in the activities of cathespin D and other lysosomal hydrolases. It is suggested that reduced lysosomal proteolytic capacity may contribute to cycloheximide-induced inhibition of protein degradation.", "contents": "Reduction by cycloheximide of lysosomal proteolytic enzyme activity and rate of protein degradation in organ-cultured hearts. Cycloheximide, an agent whose primary action is inhibition of protein synthesis, also causes a decrease in the rate of protein degradation in cultured fetal mouse hearts. This is associated with marked decreases in the activities of cathespin D and other lysosomal hydrolases. It is suggested that reduced lysosomal proteolytic capacity may contribute to cycloheximide-induced inhibition of protein degradation.", "PMID": 971421}
{"id": "pubmed23n0032_8489", "title": "Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts.", "content": "2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.", "contents": "Isolation and characterization of 6-hydroxymethylpterin as the Crithidia growth-promoting factor from spinach chloroplasts. 2-Amino-4-hydroxypteridine compounds were isolated as the Crithidia factor from spinach chloroplasts by DEAE-Sephadex A-50, DEAE-cellulose, Sephadex G-25, and thin-layer chromatography. One of the compounds was characterized as 6-hydroxymethylpterin by gas-liquid chromatography-mass spectrometry and by comparison with authentic specimen.", "PMID": 971422}
{"id": "pubmed23n0032_8490", "title": "Enthalphy changes associated with the denaturation of collagens of different imino acid content.", "content": "The enthalpy changes associated with the denaturation of acid-soluble and insoluble collagens prepared from sheep, cod, halibut and pike skin were determined by differential scanning calorimetry. The enthalpy change associated with the soluble collagens decreased with decreasing imino acid content (from 1420 cal/mol for sheep to 736 cal/mol for cod) while the value for insoluble collagens was approximately constant at 1360 cal/mol. A possible explanation for these values in terms of the nautre of the bonds present in collagen is discussed.", "contents": "Enthalphy changes associated with the denaturation of collagens of different imino acid content. The enthalpy changes associated with the denaturation of acid-soluble and insoluble collagens prepared from sheep, cod, halibut and pike skin were determined by differential scanning calorimetry. The enthalpy change associated with the soluble collagens decreased with decreasing imino acid content (from 1420 cal/mol for sheep to 736 cal/mol for cod) while the value for insoluble collagens was approximately constant at 1360 cal/mol. A possible explanation for these values in terms of the nautre of the bonds present in collagen is discussed.", "PMID": 971423}
{"id": "pubmed23n0032_8491", "title": "The action of proteolytic enzymes on chloroplast thylakoid membranes.", "content": "Envelope- and stroma-free thylakoid membranes of Vicia faba chloroplasts were incubated with trypsin or pronase for several hours. The indigestible residue was analysed by polyacrylamide gel electrophoresis. Trypsinization resulted in a complete digestion of all proteins with the exception of the pigment-protein complexes as well as a polypeptide not yet characterized. Yet, as compared with untreated material, Complex II was found to have higher electrophoretic mobility. Electron-microscopic studies illustrate that the indigestible residue still has a preserved membrane structure. Disintegration of the thylakoid membranes by sodium dodecyl sulfate followed by trypsinization also resulted in the two complexes while all the other proteins were found to be digested. However, after removal of the lipids the protein moieties of the complexes proved to be easily digestible. From these results it is concluded that pigment-protein interaction may be an important factor in maintaining a conformation rather resistant to perturbants and proteases. In contrast to trypsin, pronase completely digested the polypeptides of the thylakoid membranes including the protein moieties of the pigment-protein complexes leaving an amorphous lipid mass. The results support the assumption that the complexes are necessary to maintain the membrane structure.", "contents": "The action of proteolytic enzymes on chloroplast thylakoid membranes. Envelope- and stroma-free thylakoid membranes of Vicia faba chloroplasts were incubated with trypsin or pronase for several hours. The indigestible residue was analysed by polyacrylamide gel electrophoresis. Trypsinization resulted in a complete digestion of all proteins with the exception of the pigment-protein complexes as well as a polypeptide not yet characterized. Yet, as compared with untreated material, Complex II was found to have higher electrophoretic mobility. Electron-microscopic studies illustrate that the indigestible residue still has a preserved membrane structure. Disintegration of the thylakoid membranes by sodium dodecyl sulfate followed by trypsinization also resulted in the two complexes while all the other proteins were found to be digested. However, after removal of the lipids the protein moieties of the complexes proved to be easily digestible. From these results it is concluded that pigment-protein interaction may be an important factor in maintaining a conformation rather resistant to perturbants and proteases. In contrast to trypsin, pronase completely digested the polypeptides of the thylakoid membranes including the protein moieties of the pigment-protein complexes leaving an amorphous lipid mass. The results support the assumption that the complexes are necessary to maintain the membrane structure.", "PMID": 971424}
{"id": "pubmed23n0032_8492", "title": "Kinetics of permeation of nucleotide through oil/water interface in the interaction of nucleotide with octadecylamine and dodecyl guanidine.", "content": "The transports of tritiated ATP, ADP and AMP from the aqueous to scintillator phase with and without octadecylamine (or dodecyl guanidine) have been studied by the layered scintillation method and a theory suitable for an explanation of the results has been presented. (1) Transport processes were all expressed by the first order kinetics. (2) For the simple partitioning of ATP, the reciprocal of the rate constant of the backward permeation was linear with respect to the square of the partition coefficient. (3) For the transport of nucleotide with chemical reaction, the reciprocal of the rate constant of the backward permeation was linear against the overall partition coefficient of nucleotide. (4) A theory was presented on the basis of a general diffusion equation by assuming the two-film model with potential energy near the interface. (5) The theory could explain the dependences of the permeation rates on the partition coefficients. (6) From the finding that the ratio of the apparent diffusion coefficient in aqueous to scintillator phase was much smaller than unity, the occurrence of an energy barrier at interface was suggested. For the simple partitioning of ATP, the energy barrier was not significant.", "contents": "Kinetics of permeation of nucleotide through oil/water interface in the interaction of nucleotide with octadecylamine and dodecyl guanidine. The transports of tritiated ATP, ADP and AMP from the aqueous to scintillator phase with and without octadecylamine (or dodecyl guanidine) have been studied by the layered scintillation method and a theory suitable for an explanation of the results has been presented. (1) Transport processes were all expressed by the first order kinetics. (2) For the simple partitioning of ATP, the reciprocal of the rate constant of the backward permeation was linear with respect to the square of the partition coefficient. (3) For the transport of nucleotide with chemical reaction, the reciprocal of the rate constant of the backward permeation was linear against the overall partition coefficient of nucleotide. (4) A theory was presented on the basis of a general diffusion equation by assuming the two-film model with potential energy near the interface. (5) The theory could explain the dependences of the permeation rates on the partition coefficients. (6) From the finding that the ratio of the apparent diffusion coefficient in aqueous to scintillator phase was much smaller than unity, the occurrence of an energy barrier at interface was suggested. For the simple partitioning of ATP, the energy barrier was not significant.", "PMID": 971425}
{"id": "pubmed23n0032_8493", "title": "The binding of phenol red to rabbit renal cortex.", "content": "The binding of phenol red to the microsomal fraction of rabbit kidney cortex was rapid, reversible and consisted of two independent populations of binding sites: a high affinity and low capacity class which had an association constant of 11.29 - 10(3) M-1 and a binding capacity of 2.41 mumol phenol red bound per g of protein, and a low affinity binding population with an association constant of 0.80 - 10(3) M-1 and a maximal binding capacity of 55.06 mumol per g of protein. Probenecid (0.32 mM) competitively inhibited phenol red binding to only the high affinity binding site, whereas 2,4-dinitrophenol (0.77 mM) competitively inhibited phenol red binding to both the high and the low affinity population of binding sites. The binding of phenol red was highly sensitive to the cationic composition of the medium. The affinity of phenol red to the high and the low affinity binding populations was lowered by decreasing the sodium and potassium concentrations to 19 and 6 mequiv./l, respectively; however, the maximal binding capacity was unchanged. Calcium appeared to have no effect on the phenol red binding to the microsomes. All of these considerations suggest that the high affinity phenol red binding to the microsomal fraction may represent the interaction of phenol red with the physiological receptor necessary for organic acid transport at the peritubular membrane. Phenol red binding to the low affinity binding population may indicate an intracellular binding population which contributes to the intracellular accumulation of weak organic acids.", "contents": "The binding of phenol red to rabbit renal cortex. The binding of phenol red to the microsomal fraction of rabbit kidney cortex was rapid, reversible and consisted of two independent populations of binding sites: a high affinity and low capacity class which had an association constant of 11.29 - 10(3) M-1 and a binding capacity of 2.41 mumol phenol red bound per g of protein, and a low affinity binding population with an association constant of 0.80 - 10(3) M-1 and a maximal binding capacity of 55.06 mumol per g of protein. Probenecid (0.32 mM) competitively inhibited phenol red binding to only the high affinity binding site, whereas 2,4-dinitrophenol (0.77 mM) competitively inhibited phenol red binding to both the high and the low affinity population of binding sites. The binding of phenol red was highly sensitive to the cationic composition of the medium. The affinity of phenol red to the high and the low affinity binding populations was lowered by decreasing the sodium and potassium concentrations to 19 and 6 mequiv./l, respectively; however, the maximal binding capacity was unchanged. Calcium appeared to have no effect on the phenol red binding to the microsomes. All of these considerations suggest that the high affinity phenol red binding to the microsomal fraction may represent the interaction of phenol red with the physiological receptor necessary for organic acid transport at the peritubular membrane. Phenol red binding to the low affinity binding population may indicate an intracellular binding population which contributes to the intracellular accumulation of weak organic acids.", "PMID": 971426}
{"id": "pubmed23n0032_8494", "title": "Interactions between anaesthetics and lipid mixtures. Amines.", "content": "The effect of a number of amine anaesthetics related to procaine on the temperature of lipid phase transitions has been studied using chlorophyll a as a fluorescence probe. The amines cause a reduction in the temperature of the phase transition of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine and of mixtures of these lipids. The binding of charged amines causes a build up of positive charge on the membranes, limiting the binding. Incorporation of negative charge into the lipid bilayers causes a considerable increase in the binding of the charged amines, and the effect is reversed by addition of Ca2+. Anaesthesia is suggested to arise from an increase in the proportion of lipid in the liquid crystalline phase, resulting in a conformational change in the sodium channel. Effects of the tertiary amines on nerve conduction can be understood if the negatively charged lipid in the membrane is concentrated around the sodium channel: positively charged anaesthetics will have a greater effect when applied to the inside of a nerve because of the low Ca2+ concentration inside the nerve.", "contents": "Interactions between anaesthetics and lipid mixtures. Amines. The effect of a number of amine anaesthetics related to procaine on the temperature of lipid phase transitions has been studied using chlorophyll a as a fluorescence probe. The amines cause a reduction in the temperature of the phase transition of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine and of mixtures of these lipids. The binding of charged amines causes a build up of positive charge on the membranes, limiting the binding. Incorporation of negative charge into the lipid bilayers causes a considerable increase in the binding of the charged amines, and the effect is reversed by addition of Ca2+. Anaesthesia is suggested to arise from an increase in the proportion of lipid in the liquid crystalline phase, resulting in a conformational change in the sodium channel. Effects of the tertiary amines on nerve conduction can be understood if the negatively charged lipid in the membrane is concentrated around the sodium channel: positively charged anaesthetics will have a greater effect when applied to the inside of a nerve because of the low Ca2+ concentration inside the nerve.", "PMID": 971427}
{"id": "pubmed23n0032_8495", "title": "Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities.", "content": "The composition of specialized structures present at synapses within the central nervous system was elucidated by biochemical analysis of fractions enriched in synaptic junctional complexes and postsynaptic densities. The results indicate that the synaptic junctional complex is primarily protein together with some glycoproteins. The synaptic junctional complex proteins are similar in amino acid composition to synaptic membrane proteins; they are not expecially rich in basic residues, as previously suggested. The major carbohydrates present in the synaptic junctional complex and postsynaptic density glycoproteins are mannose, galactose, and glucosamine, with lesser amounts of fucose, N-acetylneuraminic acid, and galactosamine. Comparison with the synaptic membrane fraction indicates that galactose is more concentrated in the synaptic  junctional complex and mannose in the postsynaptic density. Glucose is dramatically enriched in both these fractions. Sucrose binding during isolation may partially account for the glucose enrichment.", "contents": "Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities. The composition of specialized structures present at synapses within the central nervous system was elucidated by biochemical analysis of fractions enriched in synaptic junctional complexes and postsynaptic densities. The results indicate that the synaptic junctional complex is primarily protein together with some glycoproteins. The synaptic junctional complex proteins are similar in amino acid composition to synaptic membrane proteins; they are not expecially rich in basic residues, as previously suggested. The major carbohydrates present in the synaptic junctional complex and postsynaptic density glycoproteins are mannose, galactose, and glucosamine, with lesser amounts of fucose, N-acetylneuraminic acid, and galactosamine. Comparison with the synaptic membrane fraction indicates that galactose is more concentrated in the synaptic  junctional complex and mannose in the postsynaptic density. Glucose is dramatically enriched in both these fractions. Sucrose binding during isolation may partially account for the glucose enrichment.", "PMID": 971428}
{"id": "pubmed23n0032_8496", "title": "X-ray diffraction study of myelin structure in immature and mutant mice.", "content": "X-ray diffraction patterns were obtained from freshly dissected central and peripheral nerves of quaking, myelin synthesis deficiency (msd), and trembler mutants, as well as immature and adult normal mice. The patterns were compared with respect to strength of myelin diffraction, background scatter level, repeat period, and intensity and linewidth of Bragg reflections. The deficiency of myelin in optic nerves was found to be (in decreasing severity): quaking greater  immature greater trembler approximately normal adult; and in sciatic nerves: trembler greater immature greater quaking greater msd approximately normal adult. Repeat periods about 3 A less than that for normal adult sciatic myelin were detected in corresponding nerves from immature, quaking, and trembler mice. In some trembler sciatic nerves a second phase having a 190-200 A period and accounting for about 60% of the total ordered myelin was also evident. Comparison of electron density profiles of membrane units calculated from the repeat periods and diffracted intensities for sciatic myelins indicate structural differences at the molecular level. The main findings are: (1) quaking myelin shows a significant elevation of density in the external protein-water layer between membrane bilayers; (2) the membrane bilayer of immature myelin is approximately equal to 2 A thinner than that for normal adult; (3) the membrane bilayer of the more compact phase in trembler myelin is approximately equal to 5 A thinner than for normal; and (4) the difference in repeat periods for the two phases present in some of the trembler nerves can be accounted for predominantly by distinct membrane bilayer separations at the external boundary.", "contents": "X-ray diffraction study of myelin structure in immature and mutant mice. X-ray diffraction patterns were obtained from freshly dissected central and peripheral nerves of quaking, myelin synthesis deficiency (msd), and trembler mutants, as well as immature and adult normal mice. The patterns were compared with respect to strength of myelin diffraction, background scatter level, repeat period, and intensity and linewidth of Bragg reflections. The deficiency of myelin in optic nerves was found to be (in decreasing severity): quaking greater  immature greater trembler approximately normal adult; and in sciatic nerves: trembler greater immature greater quaking greater msd approximately normal adult. Repeat periods about 3 A less than that for normal adult sciatic myelin were detected in corresponding nerves from immature, quaking, and trembler mice. In some trembler sciatic nerves a second phase having a 190-200 A period and accounting for about 60% of the total ordered myelin was also evident. Comparison of electron density profiles of membrane units calculated from the repeat periods and diffracted intensities for sciatic myelins indicate structural differences at the molecular level. The main findings are: (1) quaking myelin shows a significant elevation of density in the external protein-water layer between membrane bilayers; (2) the membrane bilayer of immature myelin is approximately equal to 2 A thinner than that for normal adult; (3) the membrane bilayer of the more compact phase in trembler myelin is approximately equal to 5 A thinner than for normal; and (4) the difference in repeat periods for the two phases present in some of the trembler nerves can be accounted for predominantly by distinct membrane bilayer separations at the external boundary.", "PMID": 971429}
{"id": "pubmed23n0032_8497", "title": "Energetics of permeation of thin lipid membranes by ions.", "content": "The energy of an ion in a thin hydrocarbon membrane relative to its energy in a bulk aqueous phase is considered in terms of the electrostatic and surface components that may be expected to be involved. Except when diffusion activation energies are large compared to partition free energies, the latter will control permeation rate and the state of an ion having the lowest partition energy will be critical for its permeability. This minimum is found when an ion is surrounded with a thin layer of water. All ions of the same charge will tend to be at their lowest state in a sphere of water of the same size. It is concluded, therefore, that all ions of a given charge will have about the same permeability in lipid membranes.", "contents": "Energetics of permeation of thin lipid membranes by ions. The energy of an ion in a thin hydrocarbon membrane relative to its energy in a bulk aqueous phase is considered in terms of the electrostatic and surface components that may be expected to be involved. Except when diffusion activation energies are large compared to partition free energies, the latter will control permeation rate and the state of an ion having the lowest partition energy will be critical for its permeability. This minimum is found when an ion is surrounded with a thin layer of water. All ions of the same charge will tend to be at their lowest state in a sphere of water of the same size. It is concluded, therefore, that all ions of a given charge will have about the same permeability in lipid membranes.", "PMID": 971430}
{"id": "pubmed23n0032_8498", "title": "Effects of unstirred layers or transport number discontinuities on the transient and steady-state current-voltage relationships of membranes.", "content": "The effects of current-induced electrolyte accumulation and depletion on the electrical properties of a two-layered membrane system have been examined. The membrane consisted of a charged, ion permselective layer and an uncharged, non-selective layer. The model was designed to reveal the properties of membranes possessing long pores with ionic charges at one end or of ion-selective membranes bounded by highly unstirred aqueous layers. Electrolyte concentration profiles in the inert layer and their time-dependent changes were obtained from solutions of the diffusion equation under the condition of constant current. The profiles were then used to calculate the voltage developed across the membrane at various times after the current is switched on. The theoretical results are presented in the form of i-V curves with reduced coordinates that can be used to obtain time-current-voltage relationships for membranes of the type considered having any thickness of the non-selective layer and bathed in any concentration of any 1:1 electrolyte. Experimental results on a model composite membrane were in good agreement with calculations that assume that ion transport occurs only under the influence of electrical potential and concentration gradients, suggesting that in such systems, the combined effects of convection, osmosis, electro-osmosis, and concentration-dependence of diffusion coefficients, activity coefficients, and transference numbers are small. Voltage fluctuations in the form of periodic spikes were observed experimentally at the limiting current density (the current density at which the electrolyte concentration at one surface of the selective layer goes to 0). These phenomena were not seen when the current was in the direction leading to accumulation of electrolyte in the non-selective (unstirred) layer. Such composite membranes can exhibit S-shaped and N-shaped i-V curves under ramp-voltage and ramp-current clamps, respectively.", "contents": "Effects of unstirred layers or transport number discontinuities on the transient and steady-state current-voltage relationships of membranes. The effects of current-induced electrolyte accumulation and depletion on the electrical properties of a two-layered membrane system have been examined. The membrane consisted of a charged, ion permselective layer and an uncharged, non-selective layer. The model was designed to reveal the properties of membranes possessing long pores with ionic charges at one end or of ion-selective membranes bounded by highly unstirred aqueous layers. Electrolyte concentration profiles in the inert layer and their time-dependent changes were obtained from solutions of the diffusion equation under the condition of constant current. The profiles were then used to calculate the voltage developed across the membrane at various times after the current is switched on. The theoretical results are presented in the form of i-V curves with reduced coordinates that can be used to obtain time-current-voltage relationships for membranes of the type considered having any thickness of the non-selective layer and bathed in any concentration of any 1:1 electrolyte. Experimental results on a model composite membrane were in good agreement with calculations that assume that ion transport occurs only under the influence of electrical potential and concentration gradients, suggesting that in such systems, the combined effects of convection, osmosis, electro-osmosis, and concentration-dependence of diffusion coefficients, activity coefficients, and transference numbers are small. Voltage fluctuations in the form of periodic spikes were observed experimentally at the limiting current density (the current density at which the electrolyte concentration at one surface of the selective layer goes to 0). These phenomena were not seen when the current was in the direction leading to accumulation of electrolyte in the non-selective (unstirred) layer. Such composite membranes can exhibit S-shaped and N-shaped i-V curves under ramp-voltage and ramp-current clamps, respectively.", "PMID": 971431}
{"id": "pubmed23n0032_8499", "title": "Phospholipid phase transitions. Effects of n-alcohols, n-monocarboxylic acids, phenylalkyl alcohols and quaternary ammonium compounds.", "content": "The interactions of a series of alcohols, acids and quaternary ammonium salts with a phosphatidylcholine-water model biomembrane (dipalmitoyl phosphatidycholine) system have been studied using differential scanning calorimetry. In particular the effects of these molecules upon the lipid endothermic phase transitions were investigated over a range of concentrations. A variety of effects was observed. (a) Those molecules which shift or broaden the main lipid transition can also remove the pretransition endotherm. (b) n-Alcohols and n-monocarboxylic acids containing the same number of carbon atoms have very similar effects at molar concentrations up to 40%. Those molecules containing 12 or more carbon atoms raise the main lipid phase transition whilst those molecules containing 10 or less carbon atoms lower this transition temperature. (c) The phase diagram of stearoyl alcohol in the phosphatidylcholine-water system shows the formation of lipid-alcohol complexes. (d) Alkyl trimethyl ammonium bromides showed behaviour which differs considerably from n-alcohols and n-carboxylic acids of the same chain length. (e) Other alkyltrialkyl and tetraalkylammonium bromides show that a variety of effects on the lipid phase transition can be obtained. (f) With the homologous series of phenyl-alkyl alcohols from benzyl alcohol to 4-phenyl butanol increasing the number of methylenes between the terminal OH and the benzene ring leads to greater interaction between solute and bilayer. The range of different effects obtained with the compounds studied offers a means for introducing various degrees and types of perturbation into membrane systems.", "contents": "Phospholipid phase transitions. Effects of n-alcohols, n-monocarboxylic acids, phenylalkyl alcohols and quaternary ammonium compounds. The interactions of a series of alcohols, acids and quaternary ammonium salts with a phosphatidylcholine-water model biomembrane (dipalmitoyl phosphatidycholine) system have been studied using differential scanning calorimetry. In particular the effects of these molecules upon the lipid endothermic phase transitions were investigated over a range of concentrations. A variety of effects was observed. (a) Those molecules which shift or broaden the main lipid transition can also remove the pretransition endotherm. (b) n-Alcohols and n-monocarboxylic acids containing the same number of carbon atoms have very similar effects at molar concentrations up to 40%. Those molecules containing 12 or more carbon atoms raise the main lipid phase transition whilst those molecules containing 10 or less carbon atoms lower this transition temperature. (c) The phase diagram of stearoyl alcohol in the phosphatidylcholine-water system shows the formation of lipid-alcohol complexes. (d) Alkyl trimethyl ammonium bromides showed behaviour which differs considerably from n-alcohols and n-carboxylic acids of the same chain length. (e) Other alkyltrialkyl and tetraalkylammonium bromides show that a variety of effects on the lipid phase transition can be obtained. (f) With the homologous series of phenyl-alkyl alcohols from benzyl alcohol to 4-phenyl butanol increasing the number of methylenes between the terminal OH and the benzene ring leads to greater interaction between solute and bilayer. The range of different effects obtained with the compounds studied offers a means for introducing various degrees and types of perturbation into membrane systems.", "PMID": 971432}
{"id": "pubmed23n0032_8500", "title": "Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide.", "content": "The interaction and mixing of membrane components in sonicated unilamellar vesicles and also non-sonicated multilamellar vesicles prepared from highly purified phospholipids suspended in NaCl solutions has been examined. Electron microscopy and differential scanning calorimetry were used to characterize the extent and kinetics of mixing of membrane components between different vesicle populations. No appreciable fusion was detected between populations of non-sonicated phospholipid vesicles incubated in aqueous salt (NaCl) solutions. Mixing of vesicle membrane components via diffusion of phospholipid molecules between vesicles was observed in populations of negatively charged phosphatidylglycerol vesicles but similar exchange diffusion was not detected in populations of neutral phosphatidylcholine vesicles. Incubation of sonicated vesicle populations at temperatures close to or above the phospholipid transition temperature resulted in an increase in vesicle size and mixing of vesicle membrane components as determined by a gradual change in the thermotropic properties of the mixed vesicle population. The interaction of purified phospholipid vesicles was also examined in the presence of myristic acid and lysolecithin. Our results indicate that while these agents enhance mixing of vesicle membrane components, in most cases mixing probably proceeds via diffusion of phospholipid molecules rather than by fusion of entire vesicles. Increased mixing of vesicle membrane components was also produced when vesicles were prepared containing a purified hydrophobic protein (myelin proteolipid apoprotein) or were incubated in the presence of dimethylsulfoxide. In these two systems, however, the evidence suggests that mixing of membrane components results from the fusion of entire vesicles.", "contents": "Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide. The interaction and mixing of membrane components in sonicated unilamellar vesicles and also non-sonicated multilamellar vesicles prepared from highly purified phospholipids suspended in NaCl solutions has been examined. Electron microscopy and differential scanning calorimetry were used to characterize the extent and kinetics of mixing of membrane components between different vesicle populations. No appreciable fusion was detected between populations of non-sonicated phospholipid vesicles incubated in aqueous salt (NaCl) solutions. Mixing of vesicle membrane components via diffusion of phospholipid molecules between vesicles was observed in populations of negatively charged phosphatidylglycerol vesicles but similar exchange diffusion was not detected in populations of neutral phosphatidylcholine vesicles. Incubation of sonicated vesicle populations at temperatures close to or above the phospholipid transition temperature resulted in an increase in vesicle size and mixing of vesicle membrane components as determined by a gradual change in the thermotropic properties of the mixed vesicle population. The interaction of purified phospholipid vesicles was also examined in the presence of myristic acid and lysolecithin. Our results indicate that while these agents enhance mixing of vesicle membrane components, in most cases mixing probably proceeds via diffusion of phospholipid molecules rather than by fusion of entire vesicles. Increased mixing of vesicle membrane components was also produced when vesicles were prepared containing a purified hydrophobic protein (myelin proteolipid apoprotein) or were incubated in the presence of dimethylsulfoxide. In these two systems, however, the evidence suggests that mixing of membrane components results from the fusion of entire vesicles.", "PMID": 971433}
{"id": "pubmed23n0032_8501", "title": "The transport of calcium and sodium ions across bilayers induced by neutral synthetic ligands used in specific electrodes.", "content": "Neutral synthetic ligands of calcium and sodium which enter into the liquid membrane composition of the selective electrodes of these ions have been incorporated within the bilayer. The membrane conductance measured shows that each of these two ligands behave as ion carriers for calcium or sodium ions. The selectivities with respect to the other alcaline or alcaline earth ions are similar to those observed by potentiometric measurements with thick liquid membrane selective electrodes.", "contents": "The transport of calcium and sodium ions across bilayers induced by neutral synthetic ligands used in specific electrodes. Neutral synthetic ligands of calcium and sodium which enter into the liquid membrane composition of the selective electrodes of these ions have been incorporated within the bilayer. The membrane conductance measured shows that each of these two ligands behave as ion carriers for calcium or sodium ions. The selectivities with respect to the other alcaline or alcaline earth ions are similar to those observed by potentiometric measurements with thick liquid membrane selective electrodes.", "PMID": 971434}
{"id": "pubmed23n0032_8502", "title": "Effects of cholera toxin on cellular and paracellular sodium fluxes in rabbit ileum.", "content": "The diarrhea observed in patients with cholera is known to be related to secretion of water and electrolytes into the intestinal lumen. However, the exact mechanisms involved in these secretory processes have remained unclear. Although it is clear that purified toxin acts on epithelial cell metabolism, its activity on Na+ transport across intestinal mucosa is equivocal: reported either to prevent net Na+ absorption or to cause net secretion of Na+ from serosa to mucosa. Since total transmural Na+ fluxes across \"leaky\" epithelia involve very significant movement via a paracellular shunt pathway, we studied the effects of cholera toxin on the cellular and paracellular pathways of Na+ movement. Unidirectional Na+ fluxes were examined as functions of applied potential in control tissues and in tissues from the same animal treated with purified cholera toxin. Treatment of rabbit ileum in vitro with toxin simulated the cellular component of serosa-to-mucosa Na+ flux (from 2.41 +/- 0.49 muequiv./h per cm2 under control conditions to 4.71 +/- 0.43 muequiv./h per cm2 after treatment with toxin, P less than 0.01). The effect of cholera toxin on Na+ movement through the cells from mucosa to serosa appeared to be insignificant. Finally, a marked decrease in the Na+ permeability (P less than 0.01) and no detectable significant changes in transference number for Na+ of the paracellular shunt pathway were observed following treatment with cholera toxin. These results provide direct evidence for the hypothesis that purified cholera toxin stimulates active sodium secretion but has minimal effect on sodium absorption.", "contents": "Effects of cholera toxin on cellular and paracellular sodium fluxes in rabbit ileum. The diarrhea observed in patients with cholera is known to be related to secretion of water and electrolytes into the intestinal lumen. However, the exact mechanisms involved in these secretory processes have remained unclear. Although it is clear that purified toxin acts on epithelial cell metabolism, its activity on Na+ transport across intestinal mucosa is equivocal: reported either to prevent net Na+ absorption or to cause net secretion of Na+ from serosa to mucosa. Since total transmural Na+ fluxes across \"leaky\" epithelia involve very significant movement via a paracellular shunt pathway, we studied the effects of cholera toxin on the cellular and paracellular pathways of Na+ movement. Unidirectional Na+ fluxes were examined as functions of applied potential in control tissues and in tissues from the same animal treated with purified cholera toxin. Treatment of rabbit ileum in vitro with toxin simulated the cellular component of serosa-to-mucosa Na+ flux (from 2.41 +/- 0.49 muequiv./h per cm2 under control conditions to 4.71 +/- 0.43 muequiv./h per cm2 after treatment with toxin, P less than 0.01). The effect of cholera toxin on Na+ movement through the cells from mucosa to serosa appeared to be insignificant. Finally, a marked decrease in the Na+ permeability (P less than 0.01) and no detectable significant changes in transference number for Na+ of the paracellular shunt pathway were observed following treatment with cholera toxin. These results provide direct evidence for the hypothesis that purified cholera toxin stimulates active sodium secretion but has minimal effect on sodium absorption.", "PMID": 971435}
{"id": "pubmed23n0032_8503", "title": "On the susceptibility of human platelets to aggregation by concanavalin A and the effect of this lectin on their response to ADP.", "content": "Concanavalin A aggregated gel-filtered platelets in 0.9% NaCl solution signifying cross-bridging by the lectin. Aggregation of these platelets by concanavalin A was temperature dependent; it did not occur at 0-4 degrees C unless the platelets were previously trypsinized. The level of aggregation of trypsinized platelets by concanavalin A at 0-4 degrees C was similar to that of untreated platelets at 37 degrees C. It is suggested that trypsin facilitates platelet aggregation by concanavalin A at 0-4 degrees C by causing a configurational change in membrane glycoproteins which orientates concanavalin A receptor sites into positions that favour lectin cross-bridging. Concanavalin A failed to aggregate platelets in plasma. Radioisotope studies showed that the amount of [3H]concanavalin A which combined with platelets in plasma was extremely low compared with gel-filtered platelets in saline. The aggregation of Ehrlich ascites cells by concanavalin A was considerably reduced when platelet-free plasma was added to the medium suggesting that it was due to the presence of concanavalin A-reactive components in the plasma. Concanavalin A inhibited the ADP-induced aggregation of platelets suspended in plasma or in a salts solution supplemented with calcium and fibrinogen, although the inhibitory effect was more conspicuous in the latter case. The results suggests that concanavalin A produces its inhibitory effect on ADP-induced platelet aggregation by interacting with membrane glycoproteins, and this further suggests their involvement in aggregation.", "contents": "On the susceptibility of human platelets to aggregation by concanavalin A and the effect of this lectin on their response to ADP. Concanavalin A aggregated gel-filtered platelets in 0.9% NaCl solution signifying cross-bridging by the lectin. Aggregation of these platelets by concanavalin A was temperature dependent; it did not occur at 0-4 degrees C unless the platelets were previously trypsinized. The level of aggregation of trypsinized platelets by concanavalin A at 0-4 degrees C was similar to that of untreated platelets at 37 degrees C. It is suggested that trypsin facilitates platelet aggregation by concanavalin A at 0-4 degrees C by causing a configurational change in membrane glycoproteins which orientates concanavalin A receptor sites into positions that favour lectin cross-bridging. Concanavalin A failed to aggregate platelets in plasma. Radioisotope studies showed that the amount of [3H]concanavalin A which combined with platelets in plasma was extremely low compared with gel-filtered platelets in saline. The aggregation of Ehrlich ascites cells by concanavalin A was considerably reduced when platelet-free plasma was added to the medium suggesting that it was due to the presence of concanavalin A-reactive components in the plasma. Concanavalin A inhibited the ADP-induced aggregation of platelets suspended in plasma or in a salts solution supplemented with calcium and fibrinogen, although the inhibitory effect was more conspicuous in the latter case. The results suggests that concanavalin A produces its inhibitory effect on ADP-induced platelet aggregation by interacting with membrane glycoproteins, and this further suggests their involvement in aggregation.", "PMID": 971436}
{"id": "pubmed23n0032_8504", "title": "The thermodynamic degree of coupling between metabolism and sodium transport in frog skin.", "content": "The tightness of coupling between two processes is advantageously evaluated by the thermodynamic degree of coupling q, varying in absolute value from zero for uncoupled processes to unity for processes which are related stoichiometrically. Two methods for the determination of q in the active pathway in frog skin have been developed, employing amiloride to abolish active sodium transport. The values of q in 6 frog skins varied, but were always less than unity (mean 0.79 +/- 0.06 S.E. according to one method, 0.78 +/- 0.06 S.E. according to the other). This indicates that metabolism and sodium transport are incompletely coupled in this tissue even when passive transepithelial leakage pathways are taken into account.", "contents": "The thermodynamic degree of coupling between metabolism and sodium transport in frog skin. The tightness of coupling between two processes is advantageously evaluated by the thermodynamic degree of coupling q, varying in absolute value from zero for uncoupled processes to unity for processes which are related stoichiometrically. Two methods for the determination of q in the active pathway in frog skin have been developed, employing amiloride to abolish active sodium transport. The values of q in 6 frog skins varied, but were always less than unity (mean 0.79 +/- 0.06 S.E. according to one method, 0.78 +/- 0.06 S.E. according to the other). This indicates that metabolism and sodium transport are incompletely coupled in this tissue even when passive transepithelial leakage pathways are taken into account.", "PMID": 971437}
{"id": "pubmed23n0032_8505", "title": "Cell membrane fluidity: molecular modeling of particle aggregations seen in electron microscopy.", "content": "The first simulation of the fluid mosaic model is reported. Intra-membrane particles, initially \"placed\" randomly in a membrane with fluid properties, are allowed to diffuse in the plane of the membrane and to interact with one another, in a model using molecular parameters. The resulting particle aggregates are very similar to those observed in freeze-fracture electron microscopy.", "contents": "Cell membrane fluidity: molecular modeling of particle aggregations seen in electron microscopy. The first simulation of the fluid mosaic model is reported. Intra-membrane particles, initially \"placed\" randomly in a membrane with fluid properties, are allowed to diffuse in the plane of the membrane and to interact with one another, in a model using molecular parameters. The resulting particle aggregates are very similar to those observed in freeze-fracture electron microscopy.", "PMID": 971438}
{"id": "pubmed23n0032_8506", "title": "A.E. Bennett Award Paper. A kinetic analysis of 5-hydroxyindoleacetic acid excretion from rat brain and csf.", "content": "Studies of neurotransmitter kinetics based on intraventricular injections of radio-labeled metabolites have been limited by several problems, including the inability of most investigators to recover more than 45% of the infected isotope from brain homogenates within several minutes after the injection...", "contents": "A.E. Bennett Award Paper. A kinetic analysis of 5-hydroxyindoleacetic acid excretion from rat brain and csf. Studies of neurotransmitter kinetics based on intraventricular injections of radio-labeled metabolites have been limited by several problems, including the inability of most investigators to recover more than 45% of the infected isotope from brain homogenates within several minutes after the injection...", "PMID": 971444}
{"id": "pubmed23n0032_8507", "title": "Bipolar course in schizo-affective illness.", "content": "The authors undertook a chart review to examine the use of the diagnosis of schizophrenia, schizo-affective type, in clinical practice. Of 27 patients given this diagnosis over a 3-year period, 13 were found to have evidence of bipolar course in their illnesses. For both the bipolar and unipolar groups, the most striking finding in first-degree relatives was the prominence of affective conditions. The bipolar group had a statistically significant earlier age for first psychiatric treatment and previous number of hospitalizations. Symptoms noted on admission were mostly affective, and the schizophrenic symptoms reported were noted by authors to be considered overinclusive or unreliable by many clinicians. Both groups received treatment with antipsychotic and antidepressant medication. Six of 13 bipolar patients and no unipolar patients were treated with lithium carbonate. Five bipolar patients met research criteria of Feighner et al. (1972) for primary affective illness and another met the criteria for schizophreniform illness. One unipolar patient met criteria for probable primary affective illness and another met the criteria for probable schizophreniform illness. The authors concluded that the diagnosis of schizo-affective illness, as used in day to day clinical practice, does not identify a group of schizophrenic patients nor a homogeneous patient group, and when both affective and schizophrenic features appear in a patient with a bipolar illness, the diagnosis of manic-depressive illness, not schizophrenia, should be given first consideration.", "contents": "Bipolar course in schizo-affective illness. The authors undertook a chart review to examine the use of the diagnosis of schizophrenia, schizo-affective type, in clinical practice. Of 27 patients given this diagnosis over a 3-year period, 13 were found to have evidence of bipolar course in their illnesses. For both the bipolar and unipolar groups, the most striking finding in first-degree relatives was the prominence of affective conditions. The bipolar group had a statistically significant earlier age for first psychiatric treatment and previous number of hospitalizations. Symptoms noted on admission were mostly affective, and the schizophrenic symptoms reported were noted by authors to be considered overinclusive or unreliable by many clinicians. Both groups received treatment with antipsychotic and antidepressant medication. Six of 13 bipolar patients and no unipolar patients were treated with lithium carbonate. Five bipolar patients met research criteria of Feighner et al. (1972) for primary affective illness and another met the criteria for schizophreniform illness. One unipolar patient met criteria for probable primary affective illness and another met the criteria for probable schizophreniform illness. The authors concluded that the diagnosis of schizo-affective illness, as used in day to day clinical practice, does not identify a group of schizophrenic patients nor a homogeneous patient group, and when both affective and schizophrenic features appear in a patient with a bipolar illness, the diagnosis of manic-depressive illness, not schizophrenia, should be given first consideration.", "PMID": 971445}
{"id": "pubmed23n0032_8508", "title": "Visibility of the nailfold plexus and heredity.", "content": "The parents of 37 normal healthy teenagers selected on the basis of nailfold capillary patterns (16 low-plexus children and 21 high-plexus children) were examined by capillary microscopy and rated \"blindly\" for plexus visualization scores (PVS). The hypothesis that high PVS is a hereditary trait transmitted as a monogenic autosomal dominant is supported by these data. The additional hypothesis that the high PVS may be associated with a higher social class is also confirmed in this sample. The transmission of high PVS in schizophrenics, where it is frequently observed in association with more serious features of this disease, appears to follow the same mode of inheritance as in normal subjects. The importance of detecting a biological hereditary characteristic which appears to have a modifying effect on the schizophrenic process is emphasized.", "contents": "Visibility of the nailfold plexus and heredity. The parents of 37 normal healthy teenagers selected on the basis of nailfold capillary patterns (16 low-plexus children and 21 high-plexus children) were examined by capillary microscopy and rated \"blindly\" for plexus visualization scores (PVS). The hypothesis that high PVS is a hereditary trait transmitted as a monogenic autosomal dominant is supported by these data. The additional hypothesis that the high PVS may be associated with a higher social class is also confirmed in this sample. The transmission of high PVS in schizophrenics, where it is frequently observed in association with more serious features of this disease, appears to follow the same mode of inheritance as in normal subjects. The importance of detecting a biological hereditary characteristic which appears to have a modifying effect on the schizophrenic process is emphasized.", "PMID": 971446}
{"id": "pubmed23n0032_8509", "title": "14C-homovanillic acid in the cerebrospinal fluid of parkinsonian patients after intravenous 14C-L-dopa.", "content": "Six patients with Parkinson's disease and five controls were premedicated with probenecid and the peripheral decarboxylase inhibitor alpha-methyldopathydrazine (Carbidopa) before intravenous administration of 50 muc of 14C-L-dopa in tracer quantity. Seven-and-one-half hours later lumbar CSF was obtained. 14C-homovanillic acid (HVA), a major metabolite of brain dopamine, was isolated by thin-layer chromatography and measured. The statistically significant positive correlation between endogenous HVA and 14C-HVA in the entire patient group and the slightly lower values of endogenous HVA and 14C-HVA in the CSF of the parkinsonians support the assumption that the concentration of HVA in the CSF after probenecid treatment reflects brain dopamine turnover. Measurement of labeled HVA here seems to have little advantage over measurement of endogenous HVA alone.", "contents": "14C-homovanillic acid in the cerebrospinal fluid of parkinsonian patients after intravenous 14C-L-dopa. Six patients with Parkinson's disease and five controls were premedicated with probenecid and the peripheral decarboxylase inhibitor alpha-methyldopathydrazine (Carbidopa) before intravenous administration of 50 muc of 14C-L-dopa in tracer quantity. Seven-and-one-half hours later lumbar CSF was obtained. 14C-homovanillic acid (HVA), a major metabolite of brain dopamine, was isolated by thin-layer chromatography and measured. The statistically significant positive correlation between endogenous HVA and 14C-HVA in the entire patient group and the slightly lower values of endogenous HVA and 14C-HVA in the CSF of the parkinsonians support the assumption that the concentration of HVA in the CSF after probenecid treatment reflects brain dopamine turnover. Measurement of labeled HVA here seems to have little advantage over measurement of endogenous HVA alone.", "PMID": 971447}
{"id": "pubmed23n0032_8510", "title": "Effects of changes in carbon dioxide tension in abnormally prolonged contingent negative variation (CNV).", "content": "The effects of changes in pCO2 in the development and resolution of normal (return to base line within 2 sec after S2) and abnormal (return to base line in more than 2 sec after S2) CNVs were studied. Decreased pCO2 produced by hyperventilation induced a significant reduction in the duration of the post-imperative negative variation (PINV) without significantly affecting the amplitude of the CNV. Increased pCO2, by inhalation of 5% CO2, on the contrary, produced prolongation of the PINV in two subjects. Control subjects with normal CNVs did not show significant changes in the amplitude or in the duration of the CNV wave when pCO2 was altered. The results are discussed in terms of the possible independence and higher sensitivity to metabolic changes of the PINV brain generators compared with the generators of the CNV wave per se.", "contents": "Effects of changes in carbon dioxide tension in abnormally prolonged contingent negative variation (CNV). The effects of changes in pCO2 in the development and resolution of normal (return to base line within 2 sec after S2) and abnormal (return to base line in more than 2 sec after S2) CNVs were studied. Decreased pCO2 produced by hyperventilation induced a significant reduction in the duration of the post-imperative negative variation (PINV) without significantly affecting the amplitude of the CNV. Increased pCO2, by inhalation of 5% CO2, on the contrary, produced prolongation of the PINV in two subjects. Control subjects with normal CNVs did not show significant changes in the amplitude or in the duration of the CNV wave when pCO2 was altered. The results are discussed in terms of the possible independence and higher sensitivity to metabolic changes of the PINV brain generators compared with the generators of the CNV wave per se.", "PMID": 971450}
{"id": "pubmed23n0032_8511", "title": "Diagnostic implications of the B-mitten EEG pattern: relationship to primary and secondary affective dysregulation.", "content": "Previous studies indicate that the B-Mitten EEG pattern is specific to psychiatric dysfunction and rarely encountered among normal control subjects. Our previous investigations have demonstrated a significant relationship between mittens and reactive as opposed to process schizophrenic disease. Re-analyses of mitten incidence in schizophrenics and nonschizophrenics suggests needed modification of our earlier B-Mitten-schizophrenia formulation. Present considerations indicate that the B-Mitten-reactive schizophrenia association is not primary but rather that the differential process-reactive schizophrenia mitten incidence may be a secondary epiphenomenon reflecting a more fundamental underlying process. Analyses are presented suggesting that the mitten dysrhythmia relates positively to dysphoric affective dysregulation in a manner which cuts across broad diagnostic boundaries. That the mitten pattern possibly suggests as yet unclarified subcortical dysfunction associated with symptoms of affective disturbance is a tentative hypothesis offered for consideration.", "contents": "Diagnostic implications of the B-mitten EEG pattern: relationship to primary and secondary affective dysregulation. Previous studies indicate that the B-Mitten EEG pattern is specific to psychiatric dysfunction and rarely encountered among normal control subjects. Our previous investigations have demonstrated a significant relationship between mittens and reactive as opposed to process schizophrenic disease. Re-analyses of mitten incidence in schizophrenics and nonschizophrenics suggests needed modification of our earlier B-Mitten-schizophrenia formulation. Present considerations indicate that the B-Mitten-reactive schizophrenia association is not primary but rather that the differential process-reactive schizophrenia mitten incidence may be a secondary epiphenomenon reflecting a more fundamental underlying process. Analyses are presented suggesting that the mitten dysrhythmia relates positively to dysphoric affective dysregulation in a manner which cuts across broad diagnostic boundaries. That the mitten pattern possibly suggests as yet unclarified subcortical dysfunction associated with symptoms of affective disturbance is a tentative hypothesis offered for consideration.", "PMID": 971451}
{"id": "pubmed23n0032_8512", "title": "Electrophoretic pattern of red blood cell catechol-o-methyltransferase in schizophrenia and manic-depressive illness.", "content": "Human red blood cell (RBC) catechol-O-methyltransferase (COMT) was analyzed by polyacrylamide gel electrophoresis (PAGE). One major enzyme band (B) is observed after electrophoresis. In addition, a minor band (A) of COMT activity comprising no more than 25% of the total activity, is also detectable. The rate of migration during electrophoresis of both bands of RBC COMT was the same in manic depressive, schizophrenic, and normal individuals. These results did not reveal genetic variations in the COMT molecule among these three groups. Furthermore, when total RBC COMT was measured there were no statistically significant differences between schizophrenic, manic-depressive, and control individuals.", "contents": "Electrophoretic pattern of red blood cell catechol-o-methyltransferase in schizophrenia and manic-depressive illness. Human red blood cell (RBC) catechol-O-methyltransferase (COMT) was analyzed by polyacrylamide gel electrophoresis (PAGE). One major enzyme band (B) is observed after electrophoresis. In addition, a minor band (A) of COMT activity comprising no more than 25% of the total activity, is also detectable. The rate of migration during electrophoresis of both bands of RBC COMT was the same in manic depressive, schizophrenic, and normal individuals. These results did not reveal genetic variations in the COMT molecule among these three groups. Furthermore, when total RBC COMT was measured there were no statistically significant differences between schizophrenic, manic-depressive, and control individuals.", "PMID": 971452}
{"id": "pubmed23n0032_8513", "title": "Comparison of etidocaine and lidocaine for obstetrical analgesia.", "content": "Etidocaine, a relatively new local anaesthetic agent, is compared with lidocaine for use in epidural anaesthesia for vaginal delivery. The parameters compared were quality of anaesthesia, dose of drug, onset and regression of sensory blockade, complete degree of motor blockade, effect on maternal blood pressure, and foetal Apgar scores at one and five minutes. The major differences between the drugs are the lower percentage of satisfactory anaesthesia with etidocaine, the much greater variability in the time of onset of complete sensory block with etidocaine as compared to lidocaine, the more profound motor block with etidocaine, and the slightly longer duration of action of this drug. It is concluded that etidocaine has no real place in epidural anaesthesia for obstetrics.", "contents": "Comparison of etidocaine and lidocaine for obstetrical analgesia. Etidocaine, a relatively new local anaesthetic agent, is compared with lidocaine for use in epidural anaesthesia for vaginal delivery. The parameters compared were quality of anaesthesia, dose of drug, onset and regression of sensory blockade, complete degree of motor blockade, effect on maternal blood pressure, and foetal Apgar scores at one and five minutes. The major differences between the drugs are the lower percentage of satisfactory anaesthesia with etidocaine, the much greater variability in the time of onset of complete sensory block with etidocaine as compared to lidocaine, the more profound motor block with etidocaine, and the slightly longer duration of action of this drug. It is concluded that etidocaine has no real place in epidural anaesthesia for obstetrics.", "PMID": 971455}
{"id": "pubmed23n0032_8514", "title": "Components of respiratory depression after narcotic premedication in adolescents.", "content": "The effects of narcotics on ventilatory control were assessed in 13 adolescents and young adults. Both a narcotic and narcotic-phenothiazine significantly depressed the CO2 response curve. Using an occlusion pressure technique (Pm100) to evaluate those neuromuscular processes that generate forces acting on the ventilatory pump, it was found that narcotic agents reduced neuromuscular drive. In most subjects, narcotics had an additional action that contributed to the overall ventilatory depression. Using carbon dioxide to vary neuromuscular drive before and after drug administration at constant levels of neuromuscular drive the drugs reduced tidal-volume responsiveness of the pump. We conclude that narcotics impair ventilation through a combination of two effects; first, reduced neuromuscular drive, most probably due to central depression, and second, increased impedance of the ventilatory pump, most probably due to a decrease in chest-wall compliance.", "contents": "Components of respiratory depression after narcotic premedication in adolescents. The effects of narcotics on ventilatory control were assessed in 13 adolescents and young adults. Both a narcotic and narcotic-phenothiazine significantly depressed the CO2 response curve. Using an occlusion pressure technique (Pm100) to evaluate those neuromuscular processes that generate forces acting on the ventilatory pump, it was found that narcotic agents reduced neuromuscular drive. In most subjects, narcotics had an additional action that contributed to the overall ventilatory depression. Using carbon dioxide to vary neuromuscular drive before and after drug administration at constant levels of neuromuscular drive the drugs reduced tidal-volume responsiveness of the pump. We conclude that narcotics impair ventilation through a combination of two effects; first, reduced neuromuscular drive, most probably due to central depression, and second, increased impedance of the ventilatory pump, most probably due to a decrease in chest-wall compliance.", "PMID": 971454}
{"id": "pubmed23n0032_8515", "title": "Toxicity following methoxyflurane anaesthesia. IV. The role of obesity and the effect of low dose anaesthesia on fluoride metabolism and renal function.", "content": "Seven obese and five normal weight patients were studied before, during and after one hour of methoxyflurane-nitrous oxide anaesthesia during peripheral surgical operations and compared with eight patients of normal weight anaesthetized with nitrous oxide-meperidine and d-tubocurare. Estimates were made of renal function, including serum and urinary electrolytes, osmolarity, uric acid, urea and creatinine. Renal clearances for the latter three substances were also calculated. Serum and urinary inorganic and organic fluoride concentrations were measured, as were renal clearances. This low dose methoxyflurane anaesthesia resulted only in a decrease in uric acid clearance among all the measures, when compared to the meperidine-nitrous oxide controls. The clearance of uric acid remained depressed for longer in the obese patients, but otherwise they did not differ from the normal weight patients. It is possible but not proven that depressed uric acid clearance may be related to the organic fluoride metabolite and an early indicator of methoxyflurane renal toxicity. The previously documented biotransformation of methoxyflurane was seen in this study. A double peak in serum inorganic fluoride was shown in all patients but one. Rather large differences in peak levels of serum inorganic fluoride occurred. The only significant difference between the obese and normal weight patients as far as fluoride metabolism was concerned was a greater variability in the serum inorganic fluoride levels in the obese patients. It would appear that the obese patient metabolizes methoxyflurane in a quantitatively if not qualitatively different fashion than the normal weight patient, perhaps because of fatty infiltration of the liver. Caution is advised in the use of methoxyflurane for more than 90 minutes of low concentration administration in view of the unpredictability of the biotransformation.", "contents": "Toxicity following methoxyflurane anaesthesia. IV. The role of obesity and the effect of low dose anaesthesia on fluoride metabolism and renal function. Seven obese and five normal weight patients were studied before, during and after one hour of methoxyflurane-nitrous oxide anaesthesia during peripheral surgical operations and compared with eight patients of normal weight anaesthetized with nitrous oxide-meperidine and d-tubocurare. Estimates were made of renal function, including serum and urinary electrolytes, osmolarity, uric acid, urea and creatinine. Renal clearances for the latter three substances were also calculated. Serum and urinary inorganic and organic fluoride concentrations were measured, as were renal clearances. This low dose methoxyflurane anaesthesia resulted only in a decrease in uric acid clearance among all the measures, when compared to the meperidine-nitrous oxide controls. The clearance of uric acid remained depressed for longer in the obese patients, but otherwise they did not differ from the normal weight patients. It is possible but not proven that depressed uric acid clearance may be related to the organic fluoride metabolite and an early indicator of methoxyflurane renal toxicity. The previously documented biotransformation of methoxyflurane was seen in this study. A double peak in serum inorganic fluoride was shown in all patients but one. Rather large differences in peak levels of serum inorganic fluoride occurred. The only significant difference between the obese and normal weight patients as far as fluoride metabolism was concerned was a greater variability in the serum inorganic fluoride levels in the obese patients. It would appear that the obese patient metabolizes methoxyflurane in a quantitatively if not qualitatively different fashion than the normal weight patient, perhaps because of fatty infiltration of the liver. Caution is advised in the use of methoxyflurane for more than 90 minutes of low concentration administration in view of the unpredictability of the biotransformation.", "PMID": 971456}
{"id": "pubmed23n0032_8516", "title": "Effect of anaesthesia and surgery on blood sugar and carbohydrate tolerance in African children.", "content": "The effect of pre-operative starvation, anaesthesia and surgery on blood sugar levels and the handling of carbohydrate load during operation were studied in 28 Nigerian children between 2 months and 15 years of age. (1) Age and body weight were important factors influencing the relationship between duration of pre-operative fast and the pre-induction blood sugar level in children. Hypoglycaemic values occurred in 7 per cent of the subjects studied although none was clinically hypoglycaemic. (2) Halothane anaesthesia alone did not affect blood sugar levels but relaxant anaesthesia in this study caused significant rise of blood sugar. (3) There was a marked hyperglycaemic response to surgery and handling of glucose load during operation was significantly poorer than before operation.", "contents": "Effect of anaesthesia and surgery on blood sugar and carbohydrate tolerance in African children. The effect of pre-operative starvation, anaesthesia and surgery on blood sugar levels and the handling of carbohydrate load during operation were studied in 28 Nigerian children between 2 months and 15 years of age. (1) Age and body weight were important factors influencing the relationship between duration of pre-operative fast and the pre-induction blood sugar level in children. Hypoglycaemic values occurred in 7 per cent of the subjects studied although none was clinically hypoglycaemic. (2) Halothane anaesthesia alone did not affect blood sugar levels but relaxant anaesthesia in this study caused significant rise of blood sugar. (3) There was a marked hyperglycaemic response to surgery and handling of glucose load during operation was significantly poorer than before operation.", "PMID": 971457}
{"id": "pubmed23n0032_8517", "title": "[Etomidate: cardiovascular effects of a new intravenous anesthetic agent].", "content": "The cardiovascular effects of intravenous Etomidate (0.2 mg/kg) were studied in 14 surgical patients with ages varying from 49 to 90. The anaesthetic induction occurs within 10 seconds and the mean duration of anaesthesia is from 6 to 8 minutes. I.V. Etomidate causes a slight lowering of the mean arterial pressure (8.5 per cent), a negligible increase of the heart rate (2.8 per cent) and an insignificant lowering of the mean pulmonary artery pressure (7 per cent). Cardiac output and stroke volume are respectively lowered by 7.6 per cent and 10 per cent. The peripheral vascular resistance is discreetly reduced by 3.8 per cent. From these results, we believe that Etomidate seems to induce sleep with the lightest hemodynamic disturbances in comparison with other agents commonly used. However, some undesirable side effects appeared in some of our patients which can become somewhat annoying, for example myoclonic movements and pain at the point of injection.", "contents": "[Etomidate: cardiovascular effects of a new intravenous anesthetic agent]. The cardiovascular effects of intravenous Etomidate (0.2 mg/kg) were studied in 14 surgical patients with ages varying from 49 to 90. The anaesthetic induction occurs within 10 seconds and the mean duration of anaesthesia is from 6 to 8 minutes. I.V. Etomidate causes a slight lowering of the mean arterial pressure (8.5 per cent), a negligible increase of the heart rate (2.8 per cent) and an insignificant lowering of the mean pulmonary artery pressure (7 per cent). Cardiac output and stroke volume are respectively lowered by 7.6 per cent and 10 per cent. The peripheral vascular resistance is discreetly reduced by 3.8 per cent. From these results, we believe that Etomidate seems to induce sleep with the lightest hemodynamic disturbances in comparison with other agents commonly used. However, some undesirable side effects appeared in some of our patients which can become somewhat annoying, for example myoclonic movements and pain at the point of injection.", "PMID": 971458}
{"id": "pubmed23n0032_8518", "title": "Ten cases of malignant hyperthermia in Norway.", "content": "Data are presented on ten cases of anaesthesia-induced malignant hyperthermia in Norway. Seven of the patients died, three recovered. The fatal cases were all boys in the age group 11-20 years. This age and sex distribution suggests that puberty with the increase in androgens is a precipitating factor in malignant hyperthermia. One of the victims who survived was a 4 1/2-year-old pseudohermaphrodite girl with the adrenogenital syndrome. The coincidence of malignant hyperthermia in a patient with such a rare syndrome points to the excessive formation of androgens in patients with this syndrome as a predisposing factor. The indications for surgery were traumatic injuries in five cases, congenital abnormalities in three and appendicitis in two cases. These conditions in themselves may cause an increased sensitivity to suxamethonium. One patient received only hexobarbitone, halothane and suxamethonium. After the last drug jaw rigidity and temperature rise to 41.3 degrees C prompted the anaesthetist to end the anaesthetic. The fact that the patient survived proves that suxamethonium induced jaw rigidity is valuable as a warning. The absence of cardiovascular depression after procaine 3.5 g in one patient is ascribed to the correction of acidosis at the time of infusion of this drug. It is suggested that procaine should be withheld until other measures such as cooling, correction of acidosis and steroid therapy have been tried.", "contents": "Ten cases of malignant hyperthermia in Norway. Data are presented on ten cases of anaesthesia-induced malignant hyperthermia in Norway. Seven of the patients died, three recovered. The fatal cases were all boys in the age group 11-20 years. This age and sex distribution suggests that puberty with the increase in androgens is a precipitating factor in malignant hyperthermia. One of the victims who survived was a 4 1/2-year-old pseudohermaphrodite girl with the adrenogenital syndrome. The coincidence of malignant hyperthermia in a patient with such a rare syndrome points to the excessive formation of androgens in patients with this syndrome as a predisposing factor. The indications for surgery were traumatic injuries in five cases, congenital abnormalities in three and appendicitis in two cases. These conditions in themselves may cause an increased sensitivity to suxamethonium. One patient received only hexobarbitone, halothane and suxamethonium. After the last drug jaw rigidity and temperature rise to 41.3 degrees C prompted the anaesthetist to end the anaesthetic. The fact that the patient survived proves that suxamethonium induced jaw rigidity is valuable as a warning. The absence of cardiovascular depression after procaine 3.5 g in one patient is ascribed to the correction of acidosis at the time of infusion of this drug. It is suggested that procaine should be withheld until other measures such as cooling, correction of acidosis and steroid therapy have been tried.", "PMID": 971460}
{"id": "pubmed23n0032_8519", "title": "The inflation-catheter technique of ventilation during laryngoscopy.", "content": "Over the past three years the inflation-catheter technique (ICT) of controlled ventilation during general anaesthesia has proved a safe and useful method for use with laryngoscopy and other trans-oral endoscopies (most notably flexible fiberoptic bronchoscopy). The ICT allows full control of the patient's airway for the anaesthetist and exceptionally good exposure for the surgeon. In this technique ventilation of the patient is accomplished by intermittent high flow (10 ml/kg/sec) inflation of the lungs through a small (2 to 6 mm diameter) plastic orotracheal catheter. The inflation-catheter is stiffened by fixation of an intralumenal wire. Exhalation is passive around the catheter through the open airway. The Inflation Catheter Technique is readily adaptable for patients ranging from large adults to small babies by variation of catheter sizes. It can be used safely with nitrous oxide-oxygen as the ventilating gas(i.e., Bird N2O/O2 Blender) since air entrainment is not a feature. It is also readily adaptable for use with mechanical ventilation (i.e., Wolf Injectomat).", "contents": "The inflation-catheter technique of ventilation during laryngoscopy. Over the past three years the inflation-catheter technique (ICT) of controlled ventilation during general anaesthesia has proved a safe and useful method for use with laryngoscopy and other trans-oral endoscopies (most notably flexible fiberoptic bronchoscopy). The ICT allows full control of the patient's airway for the anaesthetist and exceptionally good exposure for the surgeon. In this technique ventilation of the patient is accomplished by intermittent high flow (10 ml/kg/sec) inflation of the lungs through a small (2 to 6 mm diameter) plastic orotracheal catheter. The inflation-catheter is stiffened by fixation of an intralumenal wire. Exhalation is passive around the catheter through the open airway. The Inflation Catheter Technique is readily adaptable for patients ranging from large adults to small babies by variation of catheter sizes. It can be used safely with nitrous oxide-oxygen as the ventilating gas(i.e., Bird N2O/O2 Blender) since air entrainment is not a feature. It is also readily adaptable for use with mechanical ventilation (i.e., Wolf Injectomat).", "PMID": 971461}
{"id": "pubmed23n0032_8520", "title": "A latex bag tonometer for determining the anaesthetic blood/gas partition coefficient.", "content": "Many techniques for measurement of the tissue/gas partition coefficient are valid. Each has specific advantages and shortcomings. All the methods require suitable analytic equipment and means for controlling temperature. The disadvantages of a particular technique, the ability to cope with them and the availability of the necessary apparatus are the determining factors in choosing the best method. The latex bag tonometer has a precision and ease which compares favourably with previously described techniques for measuring anaesthetic blood/gas partition coefficients. The technique was successfully used to show the effect of in vivo haemodilution on the halothane blood/gas partition coefficient.", "contents": "A latex bag tonometer for determining the anaesthetic blood/gas partition coefficient. Many techniques for measurement of the tissue/gas partition coefficient are valid. Each has specific advantages and shortcomings. All the methods require suitable analytic equipment and means for controlling temperature. The disadvantages of a particular technique, the ability to cope with them and the availability of the necessary apparatus are the determining factors in choosing the best method. The latex bag tonometer has a precision and ease which compares favourably with previously described techniques for measuring anaesthetic blood/gas partition coefficients. The technique was successfully used to show the effect of in vivo haemodilution on the halothane blood/gas partition coefficient.", "PMID": 971462}
{"id": "pubmed23n0032_8521", "title": "The selective extraction of histones from rye chromatin.", "content": "The selective extraction of histones from rye chromatin was studied using three different methods. Extractions with NaCl-phosphate buffers at pH5.5 gave results similar to those already obtained with other types of chromatin. Histone H1 was selectively extracted with 0.6 M NaCl-0.001 M PO4, while the selectivity of dissociation of the other fractions was reduced at higher NaCl concentrations. The use of phosphate-urea buffers at pH5.5 also revealed that the histones were dissociated at the same concentrations as were calf thymus histones. Histone H1 was extracted with 0.5 M PO4-1 M urea; H1, H2A, and H2B were extracted with 0.8 M PO4-2 M urea; and all histones were removed with 0.8 M PO4-5.3 M urea. It was, however, observed that the dissociated rye histones H2A and H2B were unstable in these buffers. This instability was maximum in the presence of 3 M urea, where both histones were absent from the extracted proteins and the residual nucleoproteins. Finally, a solution of 30% ethanol-0.35 M NaCl-6 M urea produced a rye nucleoprotein fraction containing only histone H1.", "contents": "The selective extraction of histones from rye chromatin. The selective extraction of histones from rye chromatin was studied using three different methods. Extractions with NaCl-phosphate buffers at pH5.5 gave results similar to those already obtained with other types of chromatin. Histone H1 was selectively extracted with 0.6 M NaCl-0.001 M PO4, while the selectivity of dissociation of the other fractions was reduced at higher NaCl concentrations. The use of phosphate-urea buffers at pH5.5 also revealed that the histones were dissociated at the same concentrations as were calf thymus histones. Histone H1 was extracted with 0.5 M PO4-1 M urea; H1, H2A, and H2B were extracted with 0.8 M PO4-2 M urea; and all histones were removed with 0.8 M PO4-5.3 M urea. It was, however, observed that the dissociated rye histones H2A and H2B were unstable in these buffers. This instability was maximum in the presence of 3 M urea, where both histones were absent from the extracted proteins and the residual nucleoproteins. Finally, a solution of 30% ethanol-0.35 M NaCl-6 M urea produced a rye nucleoprotein fraction containing only histone H1.", "PMID": 971463}
{"id": "pubmed23n0032_8522", "title": "GM1-ganglioside and lactosylceramide beta-galactosidase from rabbit brain: inhibitor and substrate competition studies.", "content": "A rabbit brain beta-galactosidase catalyzes the hydrolysis of synthetic substrates and the natural substrates Gm1-ganglioside, lactosylceramide, and asialo-Gm1-ganglioside. gamma-D-Galactonolactone competitively inhibited hydrolysis of Gm1-ganglioside, lactosylceramide, and MU-galactoside with Ki values of 0.26 mM, 0.13 mM, and 0.77 mM, respectively. From activity plots comparing the degree of inhibition to the inhibitor concentration, a single binding site for each substrate was found. NP-Galactoside inhibited the hydrolysis of Gm1-ganglioside and lactosylceramide, where as Gm1-ganglioside inhibited lactosylceramide hydrolysis. At low substrate concentrations (less than 1 mM), Gm1-ganglioside was hydrolyzed effectively in the presence of NP-galactoside, but at higher concentrations hydrolysis of the latter was preferred. Chloromercuriphenylsulfonic acid and iodoacetate were effective inhibitors of the enzyme, but N-ethylmaleimide was not. The degree of inhibition with chloromercuriphenylsulfonic acid was different for each substrate. At 0.5 mugM chloromercuriphenylsulfonic acid, all activity towards NP-galactoside, 75% towards lactosylceramide, and 25% of the Gm1-ganglioside activity was lost. Two possible models are presented to explain these results. The data favour the presence of multiple active sites in the enzyme.", "contents": "GM1-ganglioside and lactosylceramide beta-galactosidase from rabbit brain: inhibitor and substrate competition studies. A rabbit brain beta-galactosidase catalyzes the hydrolysis of synthetic substrates and the natural substrates Gm1-ganglioside, lactosylceramide, and asialo-Gm1-ganglioside. gamma-D-Galactonolactone competitively inhibited hydrolysis of Gm1-ganglioside, lactosylceramide, and MU-galactoside with Ki values of 0.26 mM, 0.13 mM, and 0.77 mM, respectively. From activity plots comparing the degree of inhibition to the inhibitor concentration, a single binding site for each substrate was found. NP-Galactoside inhibited the hydrolysis of Gm1-ganglioside and lactosylceramide, where as Gm1-ganglioside inhibited lactosylceramide hydrolysis. At low substrate concentrations (less than 1 mM), Gm1-ganglioside was hydrolyzed effectively in the presence of NP-galactoside, but at higher concentrations hydrolysis of the latter was preferred. Chloromercuriphenylsulfonic acid and iodoacetate were effective inhibitors of the enzyme, but N-ethylmaleimide was not. The degree of inhibition with chloromercuriphenylsulfonic acid was different for each substrate. At 0.5 mugM chloromercuriphenylsulfonic acid, all activity towards NP-galactoside, 75% towards lactosylceramide, and 25% of the Gm1-ganglioside activity was lost. Two possible models are presented to explain these results. The data favour the presence of multiple active sites in the enzyme.", "PMID": 971464}
{"id": "pubmed23n0032_8523", "title": "Enzymatic synthesis of C40 carotenes by cell-free preparation from Halobacterium cutirubrum.", "content": "[14C]Mevalonate or (14C)isopentenyl pyrophosphate was found to be converted to transphytoene, trans-phytofluene, lycopene, and beta-carotene by a cell-free 270 000 X g supernatant fraction prepared from Halobacterium cutirubrum cells that were broken by manual grinding with glass beads. Incubations were done under N2 in the dark at 37 degrees C in 4 M NaCl in presence of FAD, NADP, and MgCl2; ATP was also added when mevalonate was the substrate. This system was also capable of converting trans-(14C)phytoene to beta-carotene via the intermediates trans-phytofluene, zeta-carotene, neurosporene, lycopene, and gamma-carotene. Each of these labelled intermediates on incubation separately with the same enzyme system was shown to be converted to the intermediates farther down the pathway. The results of this study show that the biosynthetic pathway for the formation of C40 carotenes in H. cutirubrum proceeds as follows: isopentenyl pyrophosphate leads to trans-phytoene leads to trans-phytofluene leads to zeta-carotene leads to neurosporene leads to lycopene leads to gamma-carotene leads to beta-carotene. This pathway differs from that in higher plants in that the cis isomers of phytoene and phytofluene are not on the main pathway of carotene biosynthesis, as they are in higher plants. Furthermore, trans-phytoene, which has not been reported to have any role in higher plants, appears to be the main intermediate in carotene biosynthesis in H. cutirubrum.", "contents": "Enzymatic synthesis of C40 carotenes by cell-free preparation from Halobacterium cutirubrum. [14C]Mevalonate or (14C)isopentenyl pyrophosphate was found to be converted to transphytoene, trans-phytofluene, lycopene, and beta-carotene by a cell-free 270 000 X g supernatant fraction prepared from Halobacterium cutirubrum cells that were broken by manual grinding with glass beads. Incubations were done under N2 in the dark at 37 degrees C in 4 M NaCl in presence of FAD, NADP, and MgCl2; ATP was also added when mevalonate was the substrate. This system was also capable of converting trans-(14C)phytoene to beta-carotene via the intermediates trans-phytofluene, zeta-carotene, neurosporene, lycopene, and gamma-carotene. Each of these labelled intermediates on incubation separately with the same enzyme system was shown to be converted to the intermediates farther down the pathway. The results of this study show that the biosynthetic pathway for the formation of C40 carotenes in H. cutirubrum proceeds as follows: isopentenyl pyrophosphate leads to trans-phytoene leads to trans-phytofluene leads to zeta-carotene leads to neurosporene leads to lycopene leads to gamma-carotene leads to beta-carotene. This pathway differs from that in higher plants in that the cis isomers of phytoene and phytofluene are not on the main pathway of carotene biosynthesis, as they are in higher plants. Furthermore, trans-phytoene, which has not been reported to have any role in higher plants, appears to be the main intermediate in carotene biosynthesis in H. cutirubrum.", "PMID": 971465}
{"id": "pubmed23n0032_8524", "title": "Effect of nicotine on biosynthesis of C50 carotenoids in Halobacterium cutirubrum.", "content": "The major carotenoids in pigmented extreme halophiles are the hydroxylated C50 red pigments bacterioruberin (major, 84%) and monoanhydrobacterioruberin (minor, 14%). When cells of Halobacterium cutirubrum were grown in the presence of nicotine they accumulated lycopene and bisanhydrobacterioruberin; maximal accumulation (80% lycopene, 20% bisanhydrobacterioruberin) occurred at 3mM nicotine. Removal of nicotine resulted in the reformation of monoanhydrobacterioruberin and bacterioruberin at the expense of lycopene and bisanhydrobacterioruberin. Thus nicotine does not inhibit the addition of the two extra isoprene units plus two hydroxyl groups (at C1 and C1') to a C40 carotenoid skeleton. It does, however, inhibit the hydration steps at C3'' and C3''' that convert bisanhydrobacterioruberin to monoanhydrobacterioruberin and finally bacterioruberin. These results support the view that the C50 carotenoids can be formed from a C40 carotene, lycopene.", "contents": "Effect of nicotine on biosynthesis of C50 carotenoids in Halobacterium cutirubrum. The major carotenoids in pigmented extreme halophiles are the hydroxylated C50 red pigments bacterioruberin (major, 84%) and monoanhydrobacterioruberin (minor, 14%). When cells of Halobacterium cutirubrum were grown in the presence of nicotine they accumulated lycopene and bisanhydrobacterioruberin; maximal accumulation (80% lycopene, 20% bisanhydrobacterioruberin) occurred at 3mM nicotine. Removal of nicotine resulted in the reformation of monoanhydrobacterioruberin and bacterioruberin at the expense of lycopene and bisanhydrobacterioruberin. Thus nicotine does not inhibit the addition of the two extra isoprene units plus two hydroxyl groups (at C1 and C1') to a C40 carotenoid skeleton. It does, however, inhibit the hydration steps at C3'' and C3''' that convert bisanhydrobacterioruberin to monoanhydrobacterioruberin and finally bacterioruberin. These results support the view that the C50 carotenoids can be formed from a C40 carotene, lycopene.", "PMID": 971466}
{"id": "pubmed23n0032_8525", "title": "Reduction of pulsatile hydraulic power in the pulmonary circultation caused by moderate vasoconstriction.", "content": "Vascular input impedance and associated hydraulic power was measured in rabbit isolated lungs. The study was focused on changes in impedance and in pulsatile hydraulic power during relaxation and contraction of vascular smooth muscle. Pulsatile power was found to be at a minimum when smooth muscle tone was such that the pulmonary arterial pressure was in the physiological range, and increased both when the vessels were relaxed and further constricted. Input impedance was found to be determined mainly by the large, proximal ('extra-alveolar') arteries.", "contents": "Reduction of pulsatile hydraulic power in the pulmonary circultation caused by moderate vasoconstriction. Vascular input impedance and associated hydraulic power was measured in rabbit isolated lungs. The study was focused on changes in impedance and in pulsatile hydraulic power during relaxation and contraction of vascular smooth muscle. Pulsatile power was found to be at a minimum when smooth muscle tone was such that the pulmonary arterial pressure was in the physiological range, and increased both when the vessels were relaxed and further constricted. Input impedance was found to be determined mainly by the large, proximal ('extra-alveolar') arteries.", "PMID": 971467}
{"id": "pubmed23n0032_8526", "title": "Myocardial contractile reserve and indices of contractility.", "content": "A close examiniation of several indices of contractility derived from the ventricular pressure was made using an isolated canine heart preparation. The repsonses to single intracoronary injections of calcium chloride as well as to increasing doses of this agent were tested. From the latter, calcium dose-transient-response curves intended to reveal the extent of myocardial/contractile reserve were constructed. Indices included various extrapolations to maximal velocity of shortening at zero load, maximal value of the quotient of the first derivative and ventricular pressure, time-tension index, energy averaged power density, and power averaged rate of generation of power density. Indices were compared over the same cardiac cycles before and after administration of calcium. Most indices showed increments from 10 to 20%, except power density functions which had increments of 40 and 70%, respectively. Calcium dose-response curves were linear for most indices, but, again, the power density functions showed the steepest slopes. After severe coronary occlusion, the curves for most indices flattened and lost linearity and, presumably, this was due to loss of myocardial contractile reserve. For milder occlusions, only the power density functions showed significant flattening. The conceptual framework of a contractile myocardial reserve appears supported by these results.", "contents": "Myocardial contractile reserve and indices of contractility. A close examiniation of several indices of contractility derived from the ventricular pressure was made using an isolated canine heart preparation. The repsonses to single intracoronary injections of calcium chloride as well as to increasing doses of this agent were tested. From the latter, calcium dose-transient-response curves intended to reveal the extent of myocardial/contractile reserve were constructed. Indices included various extrapolations to maximal velocity of shortening at zero load, maximal value of the quotient of the first derivative and ventricular pressure, time-tension index, energy averaged power density, and power averaged rate of generation of power density. Indices were compared over the same cardiac cycles before and after administration of calcium. Most indices showed increments from 10 to 20%, except power density functions which had increments of 40 and 70%, respectively. Calcium dose-response curves were linear for most indices, but, again, the power density functions showed the steepest slopes. After severe coronary occlusion, the curves for most indices flattened and lost linearity and, presumably, this was due to loss of myocardial contractile reserve. For milder occlusions, only the power density functions showed significant flattening. The conceptual framework of a contractile myocardial reserve appears supported by these results.", "PMID": 971468}
{"id": "pubmed23n0032_8527", "title": "Influence of procainamide on sodium and potassium exchange and permeabilities in cultured human cells.", "content": "The effect of procainamide on membrane cation exchange was investigated using monolayer cultures of Girardi heart cells. The initial effect of procainamide (10(-6) to 10(-3) mol/litre) was to produce a prompt reduction of the passive Na influx, dose-dependent along a sigmoid log dose-response curve. This effect was complete within 3 min and thereafter showed no further time-dependent increase. Mean passive Na influx (pmol-cm-2/s) decreased from 19.1 to 17.7 (P less than 0.05) and 10.4 (P less than 0.001) in 10(-5) and 10(-3) mol/litre procainamide, respectively. No effect on active Na extrusion was noted before 3 min following exposure to the drug, after which time it progressively declined reaching a minimum value for each concentration by 6 min and remaining at this level throughout a further 60 min exposure. For each concetnration this minimum value was similar to the Na influx measured under identical conditions. Na-coupled active K influx showed a parallel pattern of inhibition. K efflux was not decreased until approximately 20 min following exposure to the drug, but once present the reduction was similar in magnitude to that in the correspondingly measured K influx. Kinetic flux analysis revealed a decrease in both PNa and PK but indicated a greater effect on PNa. The results suggest that all of the above effects could be explained on the basis of one direct action of the drug, namely, the prompt initial decrease in PNa and Na influx. All other effects noted, both active and passive, could then be secondary to this phenomenon.", "contents": "Influence of procainamide on sodium and potassium exchange and permeabilities in cultured human cells. The effect of procainamide on membrane cation exchange was investigated using monolayer cultures of Girardi heart cells. The initial effect of procainamide (10(-6) to 10(-3) mol/litre) was to produce a prompt reduction of the passive Na influx, dose-dependent along a sigmoid log dose-response curve. This effect was complete within 3 min and thereafter showed no further time-dependent increase. Mean passive Na influx (pmol-cm-2/s) decreased from 19.1 to 17.7 (P less than 0.05) and 10.4 (P less than 0.001) in 10(-5) and 10(-3) mol/litre procainamide, respectively. No effect on active Na extrusion was noted before 3 min following exposure to the drug, after which time it progressively declined reaching a minimum value for each concentration by 6 min and remaining at this level throughout a further 60 min exposure. For each concetnration this minimum value was similar to the Na influx measured under identical conditions. Na-coupled active K influx showed a parallel pattern of inhibition. K efflux was not decreased until approximately 20 min following exposure to the drug, but once present the reduction was similar in magnitude to that in the correspondingly measured K influx. Kinetic flux analysis revealed a decrease in both PNa and PK but indicated a greater effect on PNa. The results suggest that all of the above effects could be explained on the basis of one direct action of the drug, namely, the prompt initial decrease in PNa and Na influx. All other effects noted, both active and passive, could then be secondary to this phenomenon.", "PMID": 971469}
{"id": "pubmed23n0032_8528", "title": "Progressive reduction in norepinephrine overflow during cardiac sympathetic nerve stimulation in the anaesthetized dog.", "content": "The left ansa subclavia was stimulated at supramaximal intensity at a frequency of Hz in open-chest, anaesthetized dogs for periods of about 10 min. Arterial blood pressure, myocardial contractile force, and coronary sinus blood flow reached peak values after 1 or 2 min of stimulation, and then declined gradually throughout the remainder of stimulation. In hearts that averaged 159 +/- 10 (SEM) g, the norepinephrine (NE) overflow rose to a peak value of 470 +/- 128 ng/min at the end of 1 min of sympathetic stimulation in the control animals. By the 10th min of stimulation, the NE overflow had diminished to 97 +/- 24 ng/min. In animals pretreated with cocaine (5 mg/kg) or with phenoxybenzamine (5 mg/kg), the peak NE overflow rates were 288 +/- 62 and 980 +/- 148 mg/min, respectively, and the rate of NE overflow declined to levels of 104 +/- 39 and 128 +/- 30 ng/min, respectively by the 10th min of stimulation. Since there was a profound, progressive diminution in the rate of NE overflow during sustained cardiac sympathetic stimulation regardless of whether or not NE reuptake was suppressed, it is concluded that the reduction in NE overflow reflects a curtailment of neuronal release of NE rather than an acceleration of reuptake.", "contents": "Progressive reduction in norepinephrine overflow during cardiac sympathetic nerve stimulation in the anaesthetized dog. The left ansa subclavia was stimulated at supramaximal intensity at a frequency of Hz in open-chest, anaesthetized dogs for periods of about 10 min. Arterial blood pressure, myocardial contractile force, and coronary sinus blood flow reached peak values after 1 or 2 min of stimulation, and then declined gradually throughout the remainder of stimulation. In hearts that averaged 159 +/- 10 (SEM) g, the norepinephrine (NE) overflow rose to a peak value of 470 +/- 128 ng/min at the end of 1 min of sympathetic stimulation in the control animals. By the 10th min of stimulation, the NE overflow had diminished to 97 +/- 24 ng/min. In animals pretreated with cocaine (5 mg/kg) or with phenoxybenzamine (5 mg/kg), the peak NE overflow rates were 288 +/- 62 and 980 +/- 148 mg/min, respectively, and the rate of NE overflow declined to levels of 104 +/- 39 and 128 +/- 30 ng/min, respectively by the 10th min of stimulation. Since there was a profound, progressive diminution in the rate of NE overflow during sustained cardiac sympathetic stimulation regardless of whether or not NE reuptake was suppressed, it is concluded that the reduction in NE overflow reflects a curtailment of neuronal release of NE rather than an acceleration of reuptake.", "PMID": 971470}
{"id": "pubmed23n0032_8529", "title": "On-line computation of cardiac output with the thermodilution method, using a digital minicomputer.", "content": "In the present study, several techniques for calculating cardiac output were evaluated in order to find an accurate technique suitable for on-line digital computation. The thermodilution method was evaluated by different calculation techniques and by comparing these techniques with electromagnetic flowmeter values. Comparison of thermal dilution curves, manually calculated by the log-normal and the exponential assumption, showed a correlation coefficient of 0.978 between the two methods, the former values being 11% higher. The exponential method correlated very well with the technique, using a correction factor for injection errors (r = 0.999). Since the exponential technique correlated well with the electromagnetic values (r = 0.84) and since it was less complicated than the technique, using correction factors, this technique was chosen for automation. Comparison of exponential calculated thermodilution values with a digital computer and manually, showed a correlation coefficient of 0.991. Therefore, it was concluded that on-line computation of thermodilution curves improves the applicability of the termodilution techniques as a means of measuring cardiac output.", "contents": "On-line computation of cardiac output with the thermodilution method, using a digital minicomputer. In the present study, several techniques for calculating cardiac output were evaluated in order to find an accurate technique suitable for on-line digital computation. The thermodilution method was evaluated by different calculation techniques and by comparing these techniques with electromagnetic flowmeter values. Comparison of thermal dilution curves, manually calculated by the log-normal and the exponential assumption, showed a correlation coefficient of 0.978 between the two methods, the former values being 11% higher. The exponential method correlated very well with the technique, using a correction factor for injection errors (r = 0.999). Since the exponential technique correlated well with the electromagnetic values (r = 0.84) and since it was less complicated than the technique, using correction factors, this technique was chosen for automation. Comparison of exponential calculated thermodilution values with a digital computer and manually, showed a correlation coefficient of 0.991. Therefore, it was concluded that on-line computation of thermodilution curves improves the applicability of the termodilution techniques as a means of measuring cardiac output.", "PMID": 971471}
{"id": "pubmed23n0032_8530", "title": "Reflection in the systemic arterial system: effects of aortic and carotid occlusion.", "content": "Experiments were performed in seven closed-chest anaesthetized male dogs to determine the role of pulse wave reflection in the pattern of flow and pressure in the ascending aorta. Ten days after implantation of an electromagnetic flow transducer around the ascending aorta a balloon catheter was placed in the descending aorta via the femoral arteries. At the same time a tip manometer was introduced into the ascending aorta. Aortic occlusions at three different sites caused pressure pulses with secondary systolic rises and flow pulses with biphasic deceleration. Secondary rises occurred 45 +/- 9.0 ms after the initial pressure rise for high aortic occlusion; this time was 75 +/- 8.5 ms for occlusion at the level of the diaphragm and 114 +/- 16.5 ms for occlusion near the level of the renal arteries. These times approximate the times in which the pulse travels from the tip manometer to the inflated balloons and back. Forward and reflected pressure and flow waves were calculated from reflection coefficients. Aortic occlusion caused larger reflected waves and the recorded wave forms were caused by the summation of forward and backward waves, the latter contributing the secondary pressure rise and the increased flow deceleration. Occlusion of both carotid arteries showed no specific reflection site but reflected waves were larger. This increased reflection can probably be explained as the result of greater total reflection from distributed sites under increased peripheral resistance.", "contents": "Reflection in the systemic arterial system: effects of aortic and carotid occlusion. Experiments were performed in seven closed-chest anaesthetized male dogs to determine the role of pulse wave reflection in the pattern of flow and pressure in the ascending aorta. Ten days after implantation of an electromagnetic flow transducer around the ascending aorta a balloon catheter was placed in the descending aorta via the femoral arteries. At the same time a tip manometer was introduced into the ascending aorta. Aortic occlusions at three different sites caused pressure pulses with secondary systolic rises and flow pulses with biphasic deceleration. Secondary rises occurred 45 +/- 9.0 ms after the initial pressure rise for high aortic occlusion; this time was 75 +/- 8.5 ms for occlusion at the level of the diaphragm and 114 +/- 16.5 ms for occlusion near the level of the renal arteries. These times approximate the times in which the pulse travels from the tip manometer to the inflated balloons and back. Forward and reflected pressure and flow waves were calculated from reflection coefficients. Aortic occlusion caused larger reflected waves and the recorded wave forms were caused by the summation of forward and backward waves, the latter contributing the secondary pressure rise and the increased flow deceleration. Occlusion of both carotid arteries showed no specific reflection site but reflected waves were larger. This increased reflection can probably be explained as the result of greater total reflection from distributed sites under increased peripheral resistance.", "PMID": 971472}
{"id": "pubmed23n0032_8531", "title": "Effect of ischaemia on overdrive suppression in isolated, blood-perfused atrial preparations of dogs.", "content": "The magnitude of overdrive suppression of the SA nodal pacemaker activity was markedly influenced by its ischaemia which was produced by occlusion of the perfusion tubing close to the sinus node artery in the in-situ and isolated canine sino-atrial node preparations. In all isolated preparations, postdrive suppression was significantly augmented after occlusion of the perfusion system for at least 120 seconds. It is concluded that total ischaemia depresses sino-atrial node function in the atropinized canine heart and that postdrive suppression is a good index of such impaired function.", "contents": "Effect of ischaemia on overdrive suppression in isolated, blood-perfused atrial preparations of dogs. The magnitude of overdrive suppression of the SA nodal pacemaker activity was markedly influenced by its ischaemia which was produced by occlusion of the perfusion tubing close to the sinus node artery in the in-situ and isolated canine sino-atrial node preparations. In all isolated preparations, postdrive suppression was significantly augmented after occlusion of the perfusion system for at least 120 seconds. It is concluded that total ischaemia depresses sino-atrial node function in the atropinized canine heart and that postdrive suppression is a good index of such impaired function.", "PMID": 971473}
{"id": "pubmed23n0032_8532", "title": "Controls of ventricular contractility assessed by pressure-volume ration, Emax.", "content": "The present study on canine left ventricles showed that Emax which we previously proposed as a good index of both ventricular contractility and its pumping capability, decreased from 2.06 to 1.38 kPa/ml (15.5 to 10.4 mmHg/ml) via the sino-aortic baroreceptor reflex. Cerebral ischaemic response increased Emax to 3.83 kPa/ml (28.8 mmHg/ml). Emax decreased to 1.17 kPa/ml (8.8 mmHg/ml) after cardiac denervation.", "contents": "Controls of ventricular contractility assessed by pressure-volume ration, Emax. The present study on canine left ventricles showed that Emax which we previously proposed as a good index of both ventricular contractility and its pumping capability, decreased from 2.06 to 1.38 kPa/ml (15.5 to 10.4 mmHg/ml) via the sino-aortic baroreceptor reflex. Cerebral ischaemic response increased Emax to 3.83 kPa/ml (28.8 mmHg/ml). Emax decreased to 1.17 kPa/ml (8.8 mmHg/ml) after cardiac denervation.", "PMID": 971474}
{"id": "pubmed23n0032_8533", "title": "Protective effect of hyperbaric oxygen for the temporary ischaemic myocardium. Macroscopic and histological data.", "content": "A protective effect of hyperbaric oxygen immediately after reopening of occluded coronary blood flow for the temporary ischaemic myocardial muscle was studied. Thirty dogs were used in this study, and 20 dogs were sacrificed after 4 h and 10 dogs were sacrificed after 5 d. Temporary occlusion of coronary artery (from 30 min to 2 h) was produced by ligation. One group were controls and the other group were a hyperbaric group in which dogs breathed 100% oxygen at an absolute pressure of 2 atmospheres before and after release of coronary ligation. The macroscopic extent of ischaemic area was studied by using nitroblue tetrazolium and microscopic and ECG findings were examined. By breathing oxygen at high pressure immediately after reopening of occluded coronary blood flow, the ischaemic area was markedly reduced. In such cases, some myocardial muscles around the arterioles and sinusoids, even when these vessels existed in the ischaemic area, were kept in a viable state. The repair of necrotic myocardial muscles was promoted histologically. Serious arrhythmia, especially ventricular fibrillation, was also well suppressed, and the stable haemodynamic conditions were obtained during operative procedures. No harmful side effects of hyperbaric oxygen were observed. One of the most effective treatments of acute myocardial infarction involves reconstruction of the occluded coronary artery as soon as possible after the onset of myocardial infarction by using these advantages of hyperbaric oxygen.", "contents": "Protective effect of hyperbaric oxygen for the temporary ischaemic myocardium. Macroscopic and histological data. A protective effect of hyperbaric oxygen immediately after reopening of occluded coronary blood flow for the temporary ischaemic myocardial muscle was studied. Thirty dogs were used in this study, and 20 dogs were sacrificed after 4 h and 10 dogs were sacrificed after 5 d. Temporary occlusion of coronary artery (from 30 min to 2 h) was produced by ligation. One group were controls and the other group were a hyperbaric group in which dogs breathed 100% oxygen at an absolute pressure of 2 atmospheres before and after release of coronary ligation. The macroscopic extent of ischaemic area was studied by using nitroblue tetrazolium and microscopic and ECG findings were examined. By breathing oxygen at high pressure immediately after reopening of occluded coronary blood flow, the ischaemic area was markedly reduced. In such cases, some myocardial muscles around the arterioles and sinusoids, even when these vessels existed in the ischaemic area, were kept in a viable state. The repair of necrotic myocardial muscles was promoted histologically. Serious arrhythmia, especially ventricular fibrillation, was also well suppressed, and the stable haemodynamic conditions were obtained during operative procedures. No harmful side effects of hyperbaric oxygen were observed. One of the most effective treatments of acute myocardial infarction involves reconstruction of the occluded coronary artery as soon as possible after the onset of myocardial infarction by using these advantages of hyperbaric oxygen.", "PMID": 971475}
{"id": "pubmed23n0032_8534", "title": "Physiological disposition of verapamil in man.", "content": "14C-D,L-verapamil was administered intravenously (10 mg) and orally (80 mg) to five volunteer patients. Plasma concentrations of verapamil, which were determined by mass fragmentography, declined bi-exponentially with half-lives of the chi-phase ranging from 18 to 35 min and of the beta-phase from 170 to 440 min. The apparent volume of distribution ranged from 270 to 460 litre and plasma clearance from 730 to 1980 ml/min. Following oral administration absorption was almost complete as judged from the area under the curve (AUC) of 14C-activity and cumulative urinary excretion of 14C. After intravenous infusion of verapamil about 80% of the radioactivity administered could be recovered in urine and faeces within 5 d. Despite its almost complete absorption after oral administration verapamil was shown to undergo extensive first pass metabolism as the bioavailability was only 10 to 22%. Rapid biotransformation had occurred as only a small percentage of AUC of 14C was seen to correspond to unchanged verapamil after both intravenous and oral administration.", "contents": "Physiological disposition of verapamil in man. 14C-D,L-verapamil was administered intravenously (10 mg) and orally (80 mg) to five volunteer patients. Plasma concentrations of verapamil, which were determined by mass fragmentography, declined bi-exponentially with half-lives of the chi-phase ranging from 18 to 35 min and of the beta-phase from 170 to 440 min. The apparent volume of distribution ranged from 270 to 460 litre and plasma clearance from 730 to 1980 ml/min. Following oral administration absorption was almost complete as judged from the area under the curve (AUC) of 14C-activity and cumulative urinary excretion of 14C. After intravenous infusion of verapamil about 80% of the radioactivity administered could be recovered in urine and faeces within 5 d. Despite its almost complete absorption after oral administration verapamil was shown to undergo extensive first pass metabolism as the bioavailability was only 10 to 22%. Rapid biotransformation had occurred as only a small percentage of AUC of 14C was seen to correspond to unchanged verapamil after both intravenous and oral administration.", "PMID": 971476}
{"id": "pubmed23n0032_8535", "title": "Nuclear transcripts larger than the cytoplasmic mRNAs are specified by segments of the adenovirus genome coding for early functions.", "content": "Nuclear viral RNAs synthesized early in productive infection with adenovirus 2 were analyzed by hybridization to specific viral DNA fragments. Radioactive RNAs were subjected to electrophoresis in polyacrylamide gels containing 98% formamide, and the fractionated RNA was hybridized to specific DNA fragments generated by cleavage with endo R-Eco Ri or endo R-Sma l. The viral genes expressed early in infection are located in four different segments of the genome. When nuclear RNA was hybridized to DNA fragments representing each of these segments, discrete RNA size classes were detected. For each of these four regions of the genome, some of the discrete nuclear RNAs were larger than the cytoplasmic mRNAs. As calculated from electrophoretic mobility, the molecular weight of these nuclear RNA size classes ranged from 15% to several fold greater than that of the corresponding cytoplasmic mRNAs. Hybridization-inhibition experiments were performed to analyze the sequences present in one of these larger nuclear transcripts, a 22S RNA transcribed from the left-hand end of the genome. This 22S nuclear RNA was transcribed from the same strand as the viral mRNA, for it contained cytoplasmic sequences; it also contained sequences restricted to the nucleus. The larger nuclear RNAs may serve as precursors to the cytoplasmic viral mRNAs synthesized early in infection.", "contents": "Nuclear transcripts larger than the cytoplasmic mRNAs are specified by segments of the adenovirus genome coding for early functions. Nuclear viral RNAs synthesized early in productive infection with adenovirus 2 were analyzed by hybridization to specific viral DNA fragments. Radioactive RNAs were subjected to electrophoresis in polyacrylamide gels containing 98% formamide, and the fractionated RNA was hybridized to specific DNA fragments generated by cleavage with endo R-Eco Ri or endo R-Sma l. The viral genes expressed early in infection are located in four different segments of the genome. When nuclear RNA was hybridized to DNA fragments representing each of these segments, discrete RNA size classes were detected. For each of these four regions of the genome, some of the discrete nuclear RNAs were larger than the cytoplasmic mRNAs. As calculated from electrophoretic mobility, the molecular weight of these nuclear RNA size classes ranged from 15% to several fold greater than that of the corresponding cytoplasmic mRNAs. Hybridization-inhibition experiments were performed to analyze the sequences present in one of these larger nuclear transcripts, a 22S RNA transcribed from the left-hand end of the genome. This 22S nuclear RNA was transcribed from the same strand as the viral mRNA, for it contained cytoplasmic sequences; it also contained sequences restricted to the nucleus. The larger nuclear RNAs may serve as precursors to the cytoplasmic viral mRNAs synthesized early in infection.", "PMID": 971484}
{"id": "pubmed23n0032_8536", "title": "Induction of erythroid differentiation in vitro by purines and purine analogues.", "content": "The effectiveness of purines and purine analogues as inducers  of erythroid differentiation in cultured murine erythroleukemia cells has been investigated. These cell lines have previously been shown to differentiate in vitro in response to dimethylsulfoxide (DMSO) and a number of other polar solvents. Two purine analogues, 6-thioguanine and 6-mercaptopurine, as well as the naturally occuring purine, purine, hypoxanthine, are shown to be extremely potent inducers. 6-Thioguanine is effective at a concentration of 0.06 mM, 750 fold lower than the DMSO concentration required for equivalent induction. 6-Mercaptopurine and hypoxanthine are effective inducers at a concentration of approximately 2 mM. Accumulation of globin mRNA was monitored during induction with purine inducers and shown to be similar in amount to globin mRNA levels reached in DMSO-induced cultures. Induction of differentiation by all three compounds follows a similar time course to induction with DMSO. All three compounds are potent inducers of HGPRT (hypoxanthine-guanine phosphoribosyltransferase)-negative cell lines; hence incorporation of purines into DNA is not required for induction of differentiation. Comparison of these compounds with other purines and purine analogues suggests a high degree of specificity in their interaction with a cellular target.", "contents": "Induction of erythroid differentiation in vitro by purines and purine analogues. The effectiveness of purines and purine analogues as inducers  of erythroid differentiation in cultured murine erythroleukemia cells has been investigated. These cell lines have previously been shown to differentiate in vitro in response to dimethylsulfoxide (DMSO) and a number of other polar solvents. Two purine analogues, 6-thioguanine and 6-mercaptopurine, as well as the naturally occuring purine, purine, hypoxanthine, are shown to be extremely potent inducers. 6-Thioguanine is effective at a concentration of 0.06 mM, 750 fold lower than the DMSO concentration required for equivalent induction. 6-Mercaptopurine and hypoxanthine are effective inducers at a concentration of approximately 2 mM. Accumulation of globin mRNA was monitored during induction with purine inducers and shown to be similar in amount to globin mRNA levels reached in DMSO-induced cultures. Induction of differentiation by all three compounds follows a similar time course to induction with DMSO. All three compounds are potent inducers of HGPRT (hypoxanthine-guanine phosphoribosyltransferase)-negative cell lines; hence incorporation of purines into DNA is not required for induction of differentiation. Comparison of these compounds with other purines and purine analogues suggests a high degree of specificity in their interaction with a cellular target.", "PMID": 971485}
{"id": "pubmed23n0032_8537", "title": "RNA synthesis in puff 2-48BC after experimental induction in Drosophila hydei.", "content": "Puffs induced by treatment with vitamin B6 at locus 2-48BC in salivary gland cells of Drosophila hydei were isolated by micromanipulation, and the puff RNA was analyzed electrophoretically. The main RNA species migrated with a mobility of 40S. A similar RNA species was found in this puff after its induction by temperature treatment, except that in this case material with a higher mobility was also found, presumably due to the presence of growing chains. No evidence was found for polyadenylation of the RNA contained within the puff. The RNA profile of nucleoplasm of cells from glands either treated with vitamin B6 or subjected to a temperature shock also displayed a prominent peak with a mobility of 40S which was absent in extracts of nucleoplasm from control glands.", "contents": "RNA synthesis in puff 2-48BC after experimental induction in Drosophila hydei. Puffs induced by treatment with vitamin B6 at locus 2-48BC in salivary gland cells of Drosophila hydei were isolated by micromanipulation, and the puff RNA was analyzed electrophoretically. The main RNA species migrated with a mobility of 40S. A similar RNA species was found in this puff after its induction by temperature treatment, except that in this case material with a higher mobility was also found, presumably due to the presence of growing chains. No evidence was found for polyadenylation of the RNA contained within the puff. The RNA profile of nucleoplasm of cells from glands either treated with vitamin B6 or subjected to a temperature shock also displayed a prominent peak with a mobility of 40S which was absent in extracts of nucleoplasm from control glands.", "PMID": 971486}
{"id": "pubmed23n0032_8538", "title": "The expression of differentiated functions in somatic cell hybrids: retention and activation of C3 production.", "content": "Rat and mouse fibroblasts which do not secrete the third component of complement (or secrete it at a very low rate), were respectively hybridized with mouse and rat hepatoma cells which actively produce C3. These interspecific hybrids actively secrete both mouse and rat C3. The production of C3 coded by the hepatoma cell genome is thus retained, but in addition, the secretion of C3 coded by the fibroblast genome is activated. On the other hand, intraspecific hybrids have been isolated from normal diploid C3 producing cells and non-producing cells. These cell hybrids also retain the capacity to secret C3. Retention of C3 production thus does not appear to be peculiar to hybrides derived from hepatoma cells.", "contents": "The expression of differentiated functions in somatic cell hybrids: retention and activation of C3 production. Rat and mouse fibroblasts which do not secrete the third component of complement (or secrete it at a very low rate), were respectively hybridized with mouse and rat hepatoma cells which actively produce C3. These interspecific hybrids actively secrete both mouse and rat C3. The production of C3 coded by the hepatoma cell genome is thus retained, but in addition, the secretion of C3 coded by the fibroblast genome is activated. On the other hand, intraspecific hybrids have been isolated from normal diploid C3 producing cells and non-producing cells. These cell hybrids also retain the capacity to secret C3. Retention of C3 production thus does not appear to be peculiar to hybrides derived from hepatoma cells.", "PMID": 971487}
{"id": "pubmed23n0032_8539", "title": "Cultures of normal human mammary cells.", "content": "This paper describes a method of obtaining epithelial cells from large quantities of normal human breast tissue and the response of these cells in culture to lactogenic hormones. Suspensions of single cells and clusters of cells resembling normal ductal and alveolar structures were obtained by mechanical disaggregation and subsequent (3h) incubation of tissue fragments in 0.5 mg/ml collagenase. Cells rapidly attached to glass or plastic surfaces within 48 h and grew to form large colonies which maintained their epithelial appearance throughout 2 months of observation. Cell cycling as monitored by DNA synthesis was enhanced by insulin, hydrocortisone, or ovine prolactin (in concentrations of 5.0mug/ml each) at respectively 2,3 and 5 days of incubation. These results were observed in cultures derived from 3 premenopause samples of mammary tissue maintained in medium with 1% fetal calf serum. Prolactin at a concentration of 5 mug/ml induced phosphoprotein synthesis 8-fold over control values. In addition, prolactin induced morphological changes in cells including the development of distended endoplasmic reticulum, large microvilli, and the deposition of glycogen granules. These initial results led to the tentative conclusion that prolactin was sufficient to initiate some of the characteristics in cultured cells normally associated with lactating tissues.", "contents": "Cultures of normal human mammary cells. This paper describes a method of obtaining epithelial cells from large quantities of normal human breast tissue and the response of these cells in culture to lactogenic hormones. Suspensions of single cells and clusters of cells resembling normal ductal and alveolar structures were obtained by mechanical disaggregation and subsequent (3h) incubation of tissue fragments in 0.5 mg/ml collagenase. Cells rapidly attached to glass or plastic surfaces within 48 h and grew to form large colonies which maintained their epithelial appearance throughout 2 months of observation. Cell cycling as monitored by DNA synthesis was enhanced by insulin, hydrocortisone, or ovine prolactin (in concentrations of 5.0mug/ml each) at respectively 2,3 and 5 days of incubation. These results were observed in cultures derived from 3 premenopause samples of mammary tissue maintained in medium with 1% fetal calf serum. Prolactin at a concentration of 5 mug/ml induced phosphoprotein synthesis 8-fold over control values. In addition, prolactin induced morphological changes in cells including the development of distended endoplasmic reticulum, large microvilli, and the deposition of glycogen granules. These initial results led to the tentative conclusion that prolactin was sufficient to initiate some of the characteristics in cultured cells normally associated with lactating tissues.", "PMID": 971488}
{"id": "pubmed23n0032_8540", "title": "Characterization and levels of the RNA polymerases during the embryogenesis of Artemia salina.", "content": "Dormant embryos at the gastrula stage of Artemia salina contain three DNA-dependent RNA polymerases: I, II, and III. The enzymes are solubilized from whole embryos and they are separated by chromatography on DEAE Sephadex. The ratio of activities with native and denatured DNA at the optimal salt concentrations is 3.5 for RNA polymerase I, 0.1 for RNA polymerase II and 1.0 for RNA polymerase III.Mn(i2+) is more efficient than Mg(2+) for the three enzymes. RNA polymerase II is 50% inhibited by 5 ng/ml of alpha-amanitin while RNA polymerases I and III are 10% and 30% inhibited by 1 mg/ml. During the embryonic development there is am independent variation of the levels of the RNA polymerases. RNA polymerase I increases its specific activity 4-5-fold, RNA polymerase III increases 2-fold, and RNA polymerase II less than 2-fold. The increase in RNA polymerase activity may represent a mechanism to control the rate of synthesis of RNA during the embryogenesis of A. salina.", "contents": "Characterization and levels of the RNA polymerases during the embryogenesis of Artemia salina. Dormant embryos at the gastrula stage of Artemia salina contain three DNA-dependent RNA polymerases: I, II, and III. The enzymes are solubilized from whole embryos and they are separated by chromatography on DEAE Sephadex. The ratio of activities with native and denatured DNA at the optimal salt concentrations is 3.5 for RNA polymerase I, 0.1 for RNA polymerase II and 1.0 for RNA polymerase III.Mn(i2+) is more efficient than Mg(2+) for the three enzymes. RNA polymerase II is 50% inhibited by 5 ng/ml of alpha-amanitin while RNA polymerases I and III are 10% and 30% inhibited by 1 mg/ml. During the embryonic development there is am independent variation of the levels of the RNA polymerases. RNA polymerase I increases its specific activity 4-5-fold, RNA polymerase III increases 2-fold, and RNA polymerase II less than 2-fold. The increase in RNA polymerase activity may represent a mechanism to control the rate of synthesis of RNA during the embryogenesis of A. salina.", "PMID": 971489}
{"id": "pubmed23n0032_8541", "title": "Identification of lead-binding components in rat liver: in vivo study.", "content": "In vivo experiments using 203Pb and radioactively labelled precursors such as [14C] arginine and [3H] tryptophan were performed to identify lead binding components in rat liver. The distribution of lead in 9 tissues and the intracellular distribution in liver and kidney was also investigated. Male rats were injected intravenously with 18 mug of 203Pb/rat and the 203Pb radioactivity was measured in whole tissues as well as in nuclei, mitochondria, lysosomes, microsomes and soluble fractions obtained by centrifugation of liver and kidney homogenates. The subcellular fractions from liver were purified and fractionated into macromolecular components by ultracentrifugation, gel filtration, ion exchange chromatography and solvent extraction. Nuclei were fractionated into membranes, chromatin proteins (histone and residual non-histone proteins) and DNA. Most of the lead was detected in the nuclear membrane fraction bound exclusively to membrane proteins and absent in phospholipids. The intranuclear lead was associated with histone fractions and other basic or very weakly acid proteins as indicated by the incorporation of [14C] arginine and [3H] tryptophan. Lead was present in the chromatographically purified DNA fraction but whether lead was really bound to the nucleic acid was not determined. Mitochondria were fractionated into heavy, soluble and light subfractions representing the inner membranes, the intramitochondrial matrix and the outer membranes respectively. These subfractions contained appreciable quantities of lead. No appreciable lead was present in lipids of the mitochondrial membranes. Significant quantities of lead were associated with the endoplasmic reticulum. Fractionation of microsomes into rough and smooth membranes showed that lead was almost exclusively bound to membranes of rough-surfaced microsomes associated with the heavy rough membrane subfraction. No significant lead was present in the free polysome subfraction or in lipids from the endoplasmic reticulum. More than one lead binding site was identified in the soluble fraction, the high molecular weight components representing the most important lead binding site.", "contents": "Identification of lead-binding components in rat liver: in vivo study. In vivo experiments using 203Pb and radioactively labelled precursors such as [14C] arginine and [3H] tryptophan were performed to identify lead binding components in rat liver. The distribution of lead in 9 tissues and the intracellular distribution in liver and kidney was also investigated. Male rats were injected intravenously with 18 mug of 203Pb/rat and the 203Pb radioactivity was measured in whole tissues as well as in nuclei, mitochondria, lysosomes, microsomes and soluble fractions obtained by centrifugation of liver and kidney homogenates. The subcellular fractions from liver were purified and fractionated into macromolecular components by ultracentrifugation, gel filtration, ion exchange chromatography and solvent extraction. Nuclei were fractionated into membranes, chromatin proteins (histone and residual non-histone proteins) and DNA. Most of the lead was detected in the nuclear membrane fraction bound exclusively to membrane proteins and absent in phospholipids. The intranuclear lead was associated with histone fractions and other basic or very weakly acid proteins as indicated by the incorporation of [14C] arginine and [3H] tryptophan. Lead was present in the chromatographically purified DNA fraction but whether lead was really bound to the nucleic acid was not determined. Mitochondria were fractionated into heavy, soluble and light subfractions representing the inner membranes, the intramitochondrial matrix and the outer membranes respectively. These subfractions contained appreciable quantities of lead. No appreciable lead was present in lipids of the mitochondrial membranes. Significant quantities of lead were associated with the endoplasmic reticulum. Fractionation of microsomes into rough and smooth membranes showed that lead was almost exclusively bound to membranes of rough-surfaced microsomes associated with the heavy rough membrane subfraction. No significant lead was present in the free polysome subfraction or in lipids from the endoplasmic reticulum. More than one lead binding site was identified in the soluble fraction, the high molecular weight components representing the most important lead binding site.", "PMID": 971513}
{"id": "pubmed23n0032_8542", "title": "Relationship between induction of aryl hydrocarbon hydroxylase and de novo synthesis of cytochrome P-448 (P1-450) in mice.", "content": "Phenobarbital, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT), benzpyrene, 3-methylcholanthrene (3-MC) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were administered i.p. for 1 or 3 days to genetically \"responsive\" (C57BL/6J) and genetically \"non-responsive\" (DBA/2J) mice. 3-MC or benzpyrene stimulated aryl hydrocarbon hydroxylase (AHH) activity in C57BL/6J (B6) mice but not in DBA/2J (D2) mice. TCDD induced AHH activity in both B6 and D2 mice. Time-course studies showed that in the first 12 h after a single injection of 3-MC to B6 mice there was no shift in the reduced cytochrome P-450-CO complex absorption spectra from 450 to 448 nm, although AHH activity increased 4-5 times over (above) that of the control group. The relationship between induction of AHH activity by polycyclic hydrocarbons in B6 mice and the concomitant synthesis of cytochrome P-448 is discussed.", "contents": "Relationship between induction of aryl hydrocarbon hydroxylase and de novo synthesis of cytochrome P-448 (P1-450) in mice. Phenobarbital, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT), benzpyrene, 3-methylcholanthrene (3-MC) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were administered i.p. for 1 or 3 days to genetically \"responsive\" (C57BL/6J) and genetically \"non-responsive\" (DBA/2J) mice. 3-MC or benzpyrene stimulated aryl hydrocarbon hydroxylase (AHH) activity in C57BL/6J (B6) mice but not in DBA/2J (D2) mice. TCDD induced AHH activity in both B6 and D2 mice. Time-course studies showed that in the first 12 h after a single injection of 3-MC to B6 mice there was no shift in the reduced cytochrome P-450-CO complex absorption spectra from 450 to 448 nm, although AHH activity increased 4-5 times over (above) that of the control group. The relationship between induction of AHH activity by polycyclic hydrocarbons in B6 mice and the concomitant synthesis of cytochrome P-448 is discussed.", "PMID": 971515}
{"id": "pubmed23n0032_8543", "title": "Mutation of human lymphoblasts by methylnitrosourea.", "content": "The lag in phenotype expression of methylnitrosourea(MNU)-induced mutation to 6-thioguanine (6TG) resistance has been studied in a diploid human lymphoblastoid cell line. We find that a considerable period (8-12 days) elapses before new mutants appear in treated cultures; after 2 weeks, however, a stable maximum fraction is attained, as would be expected for a genetic mutation. We present preliminary data linking this phenotypic lag to the slow degradation rate of hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and to an apparent requirement for very low (less than 0.2% normal) cellular HGPRT content in order for cells to be resistant to 10 mug 6TG/ml. A series of reconstruction experiments are presented, the results of which support the conclusion that selective pressures in the assay procedure do not bias the quantitative estimates of induced mutant fraction.", "contents": "Mutation of human lymphoblasts by methylnitrosourea. The lag in phenotype expression of methylnitrosourea(MNU)-induced mutation to 6-thioguanine (6TG) resistance has been studied in a diploid human lymphoblastoid cell line. We find that a considerable period (8-12 days) elapses before new mutants appear in treated cultures; after 2 weeks, however, a stable maximum fraction is attained, as would be expected for a genetic mutation. We present preliminary data linking this phenotypic lag to the slow degradation rate of hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and to an apparent requirement for very low (less than 0.2% normal) cellular HGPRT content in order for cells to be resistant to 10 mug 6TG/ml. A series of reconstruction experiments are presented, the results of which support the conclusion that selective pressures in the assay procedure do not bias the quantitative estimates of induced mutant fraction.", "PMID": 971516}
{"id": "pubmed23n0032_8544", "title": "Influence of dietary selenite on the binding characteristics of rat serum proteins to mercurial compounds.", "content": "The effect of dietary selenite on the binding characteristics of serum proteins was investigated with rats. In the control serum, the maximal binding of phenylmercuric acetate (PMA) and methylmercuric chloride (MMC) to rat serum protein was approx. 18 and 9 nmoles per mg protein, respectively. The binding of Hg2+ was biphasic and it did not reach a maximum at the concentrations used. Selenite treatment caused a reduction in binding capacity of serum proteins to Hg2+ and PMA, and an increase in the binding affinities. However, there were no such changes for the binding of MMC. Selenite protection from mercury toxicity, therefore, acts not only via a change in tissue distribution and a change in the formation of seleno-proteins but, also, via a change in the binding characteristics to some mercury compounds. In the case of methylmercury, a different mechanism of protection must exist as the modification of tissue distribution, its binding to subcellular and soluble proteins and the binding characteristics remained equivocal.", "contents": "Influence of dietary selenite on the binding characteristics of rat serum proteins to mercurial compounds. The effect of dietary selenite on the binding characteristics of serum proteins was investigated with rats. In the control serum, the maximal binding of phenylmercuric acetate (PMA) and methylmercuric chloride (MMC) to rat serum protein was approx. 18 and 9 nmoles per mg protein, respectively. The binding of Hg2+ was biphasic and it did not reach a maximum at the concentrations used. Selenite treatment caused a reduction in binding capacity of serum proteins to Hg2+ and PMA, and an increase in the binding affinities. However, there were no such changes for the binding of MMC. Selenite protection from mercury toxicity, therefore, acts not only via a change in tissue distribution and a change in the formation of seleno-proteins but, also, via a change in the binding characteristics to some mercury compounds. In the case of methylmercury, a different mechanism of protection must exist as the modification of tissue distribution, its binding to subcellular and soluble proteins and the binding characteristics remained equivocal.", "PMID": 971517}
{"id": "pubmed23n0032_8545", "title": "Mouse liver and lung glutathione s-epoxide transferase: effects of phenobarbital and 3-methylcholanthrene administration.", "content": "The effects of 3-methylcholanthrene (3MC) and phenobarbital (PB) administration on the levels of glutathione-S-epoxide transferase activity in supernatant preparations of liver and lung were studied in a number of different strains of mice, C57Bl/6, C3H, C3H-f, Balb/c-, A+ and DBA/2+. Three epoxide substrates, 3MC-11,12-oxide, styrene oxide (SO) and 3,3,3-trichloro-1,2-epoxypropane (TCPO), were employed in this investigation. PB administration (75 mg/kg body weight for 3 days) resulted in 13-57% increases in enzyme activity in the liver supernatant but was ineffective in inducing activity in lung. 3MC administration (40 mg/kg body weight for 2 days) on the other hand was without any effect on glutathione-S-epoxide transferase activity in both liver and lung.", "contents": "Mouse liver and lung glutathione s-epoxide transferase: effects of phenobarbital and 3-methylcholanthrene administration. The effects of 3-methylcholanthrene (3MC) and phenobarbital (PB) administration on the levels of glutathione-S-epoxide transferase activity in supernatant preparations of liver and lung were studied in a number of different strains of mice, C57Bl/6, C3H, C3H-f, Balb/c-, A+ and DBA/2+. Three epoxide substrates, 3MC-11,12-oxide, styrene oxide (SO) and 3,3,3-trichloro-1,2-epoxypropane (TCPO), were employed in this investigation. PB administration (75 mg/kg body weight for 3 days) resulted in 13-57% increases in enzyme activity in the liver supernatant but was ineffective in inducing activity in lung. 3MC administration (40 mg/kg body weight for 2 days) on the other hand was without any effect on glutathione-S-epoxide transferase activity in both liver and lung.", "PMID": 971518}
{"id": "pubmed23n0032_8546", "title": "Identification of the methyl phosphotriester of thymidylyl (3',5')thymidine as a product from reaction of DNA with the carcinogen N-methyl-N-nitrosourea.", "content": "The methyl phosphotriester of thymidylyl(3'-5')thymidine, Tp(Me)T, was obtained as a product of enzymic digestion of N-[14C]methyl-N-nitrosourea-methylated DNA or of N-methyl-N-nitrosourea-methylated [14C]thymine-labelled DNA. The identity of the 14C-labelled Tp(Me)T products was shown by co-chromatography of the 14C-labelled enzymic digests with synthetic Tp(Me)T on Dowex 50 (NH4+ form, eluted at pH 8), and by co-chromatography, on silica gel in 3 solvent systems, of the Tp(Me)T-containing fractions from the Dowex 50 column. This identity was confirmed by showing that the 14C-labelled DNA-derived products hydrolysed in 0.1 M sodium hydroxide at 37 degrees C at a rate identical with that of synthetic Tp(Me)T, and gave the four expected UV-absorbing products (thymidine, thymidylyl(3'-5')thymidine, and the methyl esters of 3'- and 5'-TMP) in the same ratios as the authentic triester.", "contents": "Identification of the methyl phosphotriester of thymidylyl (3',5')thymidine as a product from reaction of DNA with the carcinogen N-methyl-N-nitrosourea. The methyl phosphotriester of thymidylyl(3'-5')thymidine, Tp(Me)T, was obtained as a product of enzymic digestion of N-[14C]methyl-N-nitrosourea-methylated DNA or of N-methyl-N-nitrosourea-methylated [14C]thymine-labelled DNA. The identity of the 14C-labelled Tp(Me)T products was shown by co-chromatography of the 14C-labelled enzymic digests with synthetic Tp(Me)T on Dowex 50 (NH4+ form, eluted at pH 8), and by co-chromatography, on silica gel in 3 solvent systems, of the Tp(Me)T-containing fractions from the Dowex 50 column. This identity was confirmed by showing that the 14C-labelled DNA-derived products hydrolysed in 0.1 M sodium hydroxide at 37 degrees C at a rate identical with that of synthetic Tp(Me)T, and gave the four expected UV-absorbing products (thymidine, thymidylyl(3'-5')thymidine, and the methyl esters of 3'- and 5'-TMP) in the same ratios as the authentic triester.", "PMID": 971519}
{"id": "pubmed23n0032_8547", "title": "A comparison of the sulphite and thrombin methods for measuring fibrinogen concentration of plasma from patients undergoing thrombolytic therapy.", "content": "A thrombin method for the determination of fibrinogen concentration has been compared with a non-enzymatic sulphite precipitation method on plasma from patients undergoing thrombolytic therapy with an intermittent plasminogen/streptokinase regime. There was very good agreement between the methods even when the determinations were made in the presence of high levels of fibrinogen degradation products (FDP), provided that the thrombin clottable fibrinogen was greater than 1 mg/ml. However, after extensive fibrinogen depletion, when the thrombin method underestimates fibrinogen concentration due to significant clot inhibition by FDP, an overestimate due to co-precipitation of fragments X and Y will be obtained by the sulphite method. This suggests that the simultaneous application of both techniques may provide a simple method of assessing the presence of high levels of anticoagulant FDP in various clinical disorders.", "contents": "A comparison of the sulphite and thrombin methods for measuring fibrinogen concentration of plasma from patients undergoing thrombolytic therapy. A thrombin method for the determination of fibrinogen concentration has been compared with a non-enzymatic sulphite precipitation method on plasma from patients undergoing thrombolytic therapy with an intermittent plasminogen/streptokinase regime. There was very good agreement between the methods even when the determinations were made in the presence of high levels of fibrinogen degradation products (FDP), provided that the thrombin clottable fibrinogen was greater than 1 mg/ml. However, after extensive fibrinogen depletion, when the thrombin method underestimates fibrinogen concentration due to significant clot inhibition by FDP, an overestimate due to co-precipitation of fragments X and Y will be obtained by the sulphite method. This suggests that the simultaneous application of both techniques may provide a simple method of assessing the presence of high levels of anticoagulant FDP in various clinical disorders.", "PMID": 971520}
{"id": "pubmed23n0032_8548", "title": "[Bisalbuminaemia in pancreatitis: structural modifications of human serum albumin by proteolytic enzymes of the pancreas (author's transl)].", "content": "Bisalbuminaemia in pancreatitis is a transient abnormality related to the presence, on electrophoresis of the serum, of a fast-moving albumin; this abnormal form is also found, in large amounts, in the ascitic or pleural effusions of the patients. Experiments reported here indicate clearly that the fast albumin can be produced by a degradation of normal serum albumin by the proteolytic enzymes of the pancreas (chymotrypsin or elastase in association with carboxypeptidases A and B). Stuctural analysis of the isolated fast albumin of the patients shows that the C-terminal end of the molecule is different from normal serum albumin, which can be understood by a limited enzymatic degradation by chymotrypsin or elastase followed by the action of carboxypeptidases. The discovery of bisalbuminaemia in a patient affected by pancreatitis is suspicious of the presence of an ascitic or pleural effusion and of a pancreatic pseudo-cyst with a fistula emerging in the effusion.", "contents": "[Bisalbuminaemia in pancreatitis: structural modifications of human serum albumin by proteolytic enzymes of the pancreas (author's transl)]. Bisalbuminaemia in pancreatitis is a transient abnormality related to the presence, on electrophoresis of the serum, of a fast-moving albumin; this abnormal form is also found, in large amounts, in the ascitic or pleural effusions of the patients. Experiments reported here indicate clearly that the fast albumin can be produced by a degradation of normal serum albumin by the proteolytic enzymes of the pancreas (chymotrypsin or elastase in association with carboxypeptidases A and B). Stuctural analysis of the isolated fast albumin of the patients shows that the C-terminal end of the molecule is different from normal serum albumin, which can be understood by a limited enzymatic degradation by chymotrypsin or elastase followed by the action of carboxypeptidases. The discovery of bisalbuminaemia in a patient affected by pancreatitis is suspicious of the presence of an ascitic or pleural effusion and of a pancreatic pseudo-cyst with a fistula emerging in the effusion.", "PMID": 971522}
{"id": "pubmed23n0032_8549", "title": "Automatic potentiometric titration and gas-liquid chromatography of underivatised free fatty acids.", "content": "A rapid and precise method is described for the determination of total free fatty acids (FFA) in the serum followed by the separation of individual FFA by gas-liquid chromatography (GLC). After the extraction the FFA are directly injected into the gas-liquid chromatograph without esterification. DEGS-PS serves as stationary phase. The esterified fatty acids such as cholesterol esters, phospholipids or triglycerides do not interfere with the FFA determined by the GLC method. This method is compared to other commonly used titrimetric and gas-chromatographic procedures. The changes of the composition of individual FFA during and after ergometer work is reported.", "contents": "Automatic potentiometric titration and gas-liquid chromatography of underivatised free fatty acids. A rapid and precise method is described for the determination of total free fatty acids (FFA) in the serum followed by the separation of individual FFA by gas-liquid chromatography (GLC). After the extraction the FFA are directly injected into the gas-liquid chromatograph without esterification. DEGS-PS serves as stationary phase. The esterified fatty acids such as cholesterol esters, phospholipids or triglycerides do not interfere with the FFA determined by the GLC method. This method is compared to other commonly used titrimetric and gas-chromatographic procedures. The changes of the composition of individual FFA during and after ergometer work is reported.", "PMID": 971523}
{"id": "pubmed23n0032_8550", "title": "Urinary albumin excretion in normal and diabetic subjects.", "content": "Using an albumin radio-immuno-assay, urinary albumin concentration and excretion rate have been measured in diabetics and control subjects overnight, after several hours ordinary activity and during two successive one hour periods of recumbency. The urine albumin concentration was relatively constant throughout each of the four collection periods. Variations in albumin excretion rate were directly related to changes in urine flow. In assessing changes in urinary albumin, concentration and urine flow should be reported as well as the calculated albumin excretion rate. In the diabetics, selected for absence of clinical proteinuria, the mean concentration of albumin did not differ significantly from that of the controls. The overnight albumin excretion rate was higher in the diabetics, but this was due to the greater volume of urine.", "contents": "Urinary albumin excretion in normal and diabetic subjects. Using an albumin radio-immuno-assay, urinary albumin concentration and excretion rate have been measured in diabetics and control subjects overnight, after several hours ordinary activity and during two successive one hour periods of recumbency. The urine albumin concentration was relatively constant throughout each of the four collection periods. Variations in albumin excretion rate were directly related to changes in urine flow. In assessing changes in urinary albumin, concentration and urine flow should be reported as well as the calculated albumin excretion rate. In the diabetics, selected for absence of clinical proteinuria, the mean concentration of albumin did not differ significantly from that of the controls. The overnight albumin excretion rate was higher in the diabetics, but this was due to the greater volume of urine.", "PMID": 971524}
{"id": "pubmed23n0032_8551", "title": "Rapid determination of urine estriol by an on-column derivatization procedure.", "content": "Estriol in pregnancy urine is determined by acid hydrolysis of steroid conjugates, followed by ether and aqueous sodium hydroxide extraction and gas chromatography. Derivatives are formed within the injection port of the gas chromatograph using tetramethylammonium hydroxide. Several samples may be processed simultaneously. Hydrolysis and extraction of a specimen are done in a single culture tube; for larger numbers of specimens, a Paton-Brown extractor may be used. With a two-column gas chromatograph, results on two specimens are available within one hour; twelve analyses may be completed within two hours.", "contents": "Rapid determination of urine estriol by an on-column derivatization procedure. Estriol in pregnancy urine is determined by acid hydrolysis of steroid conjugates, followed by ether and aqueous sodium hydroxide extraction and gas chromatography. Derivatives are formed within the injection port of the gas chromatograph using tetramethylammonium hydroxide. Several samples may be processed simultaneously. Hydrolysis and extraction of a specimen are done in a single culture tube; for larger numbers of specimens, a Paton-Brown extractor may be used. With a two-column gas chromatograph, results on two specimens are available within one hour; twelve analyses may be completed within two hours.", "PMID": 971525}
{"id": "pubmed23n0032_8552", "title": "Total and immunologically reacting muscle enolase in blood serum of Duchenne muscular dystrophy patients and carriers.", "content": "All of 12 patients with Duchenne muscular dystrophy manifested raised activity of serum enolase but only 10 showed elevation of immunologically reacting muscle enolase (IRM-enolase). Total enolase was raised in 4 of 12 mothers and one of 8 sisters of the affected subjects but IRM-enolase was elevated in only one mother. These results are consistent with the view that enolase, unlike creatine phosphokinase which showed much more pronounced increase in the serum of patients and their female relatives, is associated with a structural component of the muscle cell distinct from the presumed site of molecular abnormality in this disease.", "contents": "Total and immunologically reacting muscle enolase in blood serum of Duchenne muscular dystrophy patients and carriers. All of 12 patients with Duchenne muscular dystrophy manifested raised activity of serum enolase but only 10 showed elevation of immunologically reacting muscle enolase (IRM-enolase). Total enolase was raised in 4 of 12 mothers and one of 8 sisters of the affected subjects but IRM-enolase was elevated in only one mother. These results are consistent with the view that enolase, unlike creatine phosphokinase which showed much more pronounced increase in the serum of patients and their female relatives, is associated with a structural component of the muscle cell distinct from the presumed site of molecular abnormality in this disease.", "PMID": 971527}
{"id": "pubmed23n0032_8553", "title": "A single nonaqueous diazo reagent for the rapid determination of bilirubin in serum without lipemic interference.", "content": "A nonaqueous reagent for directly determining serum bilirubin with 3,3'-dimethoxydiphenyl-4,4'-tetrazonium chloride in acidified dimethylsulfoxide is described. Conjugated or unconjugated bilirubin may be determined after extraction in acidified chloroform-ethyl acetate. The procedure is accurate (r2 0.97; recovery 100% for total, 98% for extracted bilirubin) and precise (C.V. 3.0% at 65 mg bilirubin per 1. The reagent develops endpoint color at 590 nm in less than 2 min, and the developed absorbance is stable for several hours. The method is intended for special applications where gross lipemia is encountered. Standardization with pure bilirubin in dimethylsulfoxide or chloroform is identical to standardization with aqueous bilirubin in protein standards.", "contents": "A single nonaqueous diazo reagent for the rapid determination of bilirubin in serum without lipemic interference. A nonaqueous reagent for directly determining serum bilirubin with 3,3'-dimethoxydiphenyl-4,4'-tetrazonium chloride in acidified dimethylsulfoxide is described. Conjugated or unconjugated bilirubin may be determined after extraction in acidified chloroform-ethyl acetate. The procedure is accurate (r2 0.97; recovery 100% for total, 98% for extracted bilirubin) and precise (C.V. 3.0% at 65 mg bilirubin per 1. The reagent develops endpoint color at 590 nm in less than 2 min, and the developed absorbance is stable for several hours. The method is intended for special applications where gross lipemia is encountered. Standardization with pure bilirubin in dimethylsulfoxide or chloroform is identical to standardization with aqueous bilirubin in protein standards.", "PMID": 971528}
{"id": "pubmed23n0032_8554", "title": "Evaluation of the Vitatron \"AKES\" modification for optimal use in routine enzyme analysis.", "content": "The Vitatron has been used as designed by the manufacturer under routine laboratory conditions (Broughton, P.M.G., Buttolph, M.A., Gowenlock, A.H., Neill, D.W. and Sleutelberg, R.G. (1969) J. Clin. Pathol. 22, 278). We assessed the possibilities of the AKES with regard to determination of the activities of three enzymes: alanine transaminase (AIT), aspartate transaminase (AsT) and lactate dehydrogenase (LDH) in serum. Precision, accuracy, carry over and sample-diluent contamination were evaluated. This resulted in recommendations for optimal use in terms of capacity and precision, which were supported by computations on a mathematical model for measuring results.", "contents": "Evaluation of the Vitatron \"AKES\" modification for optimal use in routine enzyme analysis. The Vitatron has been used as designed by the manufacturer under routine laboratory conditions (Broughton, P.M.G., Buttolph, M.A., Gowenlock, A.H., Neill, D.W. and Sleutelberg, R.G. (1969) J. Clin. Pathol. 22, 278). We assessed the possibilities of the AKES with regard to determination of the activities of three enzymes: alanine transaminase (AIT), aspartate transaminase (AsT) and lactate dehydrogenase (LDH) in serum. Precision, accuracy, carry over and sample-diluent contamination were evaluated. This resulted in recommendations for optimal use in terms of capacity and precision, which were supported by computations on a mathematical model for measuring results.", "PMID": 971529}
{"id": "pubmed23n0032_8555", "title": "Studies on blood saccharoid fraction in normal and diabetic subjects.", "content": "The exact nature of the saccharoid fraction (non-glucose reducing substances) in human blood is not known. The saccharoid fraction is increased in diabetic patients and a direct relationship exists to the height of the blood sugar. Some of the constituents of the saccharoid fraction, namely, glutathione, glucuronic acid and organic phosphate are significantly increased in diabetic patients. Fructose diphosphate and ATP are increased in the patients treated with oral hypoglycemic drugs. The known constituents of the saccharoid fraction which were determined accounted only for about 48% in normal and 36% in diabetic patients. The reduced contribution of these compounds to the saccharoid fraction in diabetic patients indicates that the nature of the saccharoid fraction may be different in diabetic patients.", "contents": "Studies on blood saccharoid fraction in normal and diabetic subjects. The exact nature of the saccharoid fraction (non-glucose reducing substances) in human blood is not known. The saccharoid fraction is increased in diabetic patients and a direct relationship exists to the height of the blood sugar. Some of the constituents of the saccharoid fraction, namely, glutathione, glucuronic acid and organic phosphate are significantly increased in diabetic patients. Fructose diphosphate and ATP are increased in the patients treated with oral hypoglycemic drugs. The known constituents of the saccharoid fraction which were determined accounted only for about 48% in normal and 36% in diabetic patients. The reduced contribution of these compounds to the saccharoid fraction in diabetic patients indicates that the nature of the saccharoid fraction may be different in diabetic patients.", "PMID": 971530}
{"id": "pubmed23n0032_8556", "title": "Triglyceride lipases in acute hepatitis.", "content": "Two triglyceride lipases in postheparin plasma, the lipoprotein lipase (LPL) and the hepatic triglyceride lipase (H-TGL) were separated by heparin-sepharose affinity chromatography and studied in controls and patients during the course of acute hepatitis. All three patients had increased content of triglycerides in the low density lipoproteins, and two of them had hypertriglyceridemia. Low activities of both lipases were found in the acute stage of the disease in all three patients. Concomitantly one of the patients had absolute low lecithin: cholesterol acyltransferase (LCAT) activity, and in the two other patients a relative LCAT deficiency was present. The increased content of triglycerides in LDL that may be found in liver disease, may not only be due to low H-TGL and LPL, but also LCAT deficiency.", "contents": "Triglyceride lipases in acute hepatitis. Two triglyceride lipases in postheparin plasma, the lipoprotein lipase (LPL) and the hepatic triglyceride lipase (H-TGL) were separated by heparin-sepharose affinity chromatography and studied in controls and patients during the course of acute hepatitis. All three patients had increased content of triglycerides in the low density lipoproteins, and two of them had hypertriglyceridemia. Low activities of both lipases were found in the acute stage of the disease in all three patients. Concomitantly one of the patients had absolute low lecithin: cholesterol acyltransferase (LCAT) activity, and in the two other patients a relative LCAT deficiency was present. The increased content of triglycerides in LDL that may be found in liver disease, may not only be due to low H-TGL and LPL, but also LCAT deficiency.", "PMID": 971531}
{"id": "pubmed23n0032_8557", "title": "[Adenosine deaminase activity in human erythrocytes and lymphocytes].", "content": "A radiochromatographic method is described for measuring enzymatic activity of adenosine deaminase in human erythrocytes and lymphocytes. [8-14C]-adenosine is converted into inosine and hypoxanthine; after chromatographic separation of the products, the radioactivity is determined. The kinetic properties of the enzyme have been studied. The Km values for the erythrocyte and lymphocyte enzymes are higher as compared with purified deaminase. Optimum conditions for substrate concentration for assay were established. The mean normal activity (+/- S.E. of mean) is: for erythrocytes, 494 +/- 61; nmol min-1 ml-1; for lymphocytes- 147 +/- 0.18 nmol min-1 10(6) cellules. The mean values are higher than that given by other methods working at a lower (non-staurating) substrate concentration.", "contents": "[Adenosine deaminase activity in human erythrocytes and lymphocytes]. A radiochromatographic method is described for measuring enzymatic activity of adenosine deaminase in human erythrocytes and lymphocytes. [8-14C]-adenosine is converted into inosine and hypoxanthine; after chromatographic separation of the products, the radioactivity is determined. The kinetic properties of the enzyme have been studied. The Km values for the erythrocyte and lymphocyte enzymes are higher as compared with purified deaminase. Optimum conditions for substrate concentration for assay were established. The mean normal activity (+/- S.E. of mean) is: for erythrocytes, 494 +/- 61; nmol min-1 ml-1; for lymphocytes- 147 +/- 0.18 nmol min-1 10(6) cellules. The mean values are higher than that given by other methods working at a lower (non-staurating) substrate concentration.", "PMID": 971532}
{"id": "pubmed23n0032_8558", "title": "Stability of testosterone and androstenedione in blood and plasma samples.", "content": "In order to investigate the stability of testosterone (T) and androstenedione (Adione) in blood and plasma samples following study was undertaken. 1. Pooled blood samples were left unseparated at room temperature for varying time periods of up to 3 days before being centrifuged. Subsequent plasma T and Adione values measured by radioimmunoassay showed only small, insignificant variations. 2. Plasma, initially separated from the pooled blood sample, was left at room temperature for up to 24 h. There was no significant change in the levels of T and Adione measured over this period. There was also good correlation between steroid concentrations measured in blood and plasma. 3. T and Adione were measured in a pooled plasma sample after repeated freezing and thawing of the sample. All T values were well within the intraassay coefficient of variation; all Adione values were within the precision limits of the assay, although there was a considerable spread in individual values. It is concluded that the various handling procedures of blood or plasma have no significant effect on T and Adione levels.", "contents": "Stability of testosterone and androstenedione in blood and plasma samples. In order to investigate the stability of testosterone (T) and androstenedione (Adione) in blood and plasma samples following study was undertaken. 1. Pooled blood samples were left unseparated at room temperature for varying time periods of up to 3 days before being centrifuged. Subsequent plasma T and Adione values measured by radioimmunoassay showed only small, insignificant variations. 2. Plasma, initially separated from the pooled blood sample, was left at room temperature for up to 24 h. There was no significant change in the levels of T and Adione measured over this period. There was also good correlation between steroid concentrations measured in blood and plasma. 3. T and Adione were measured in a pooled plasma sample after repeated freezing and thawing of the sample. All T values were well within the intraassay coefficient of variation; all Adione values were within the precision limits of the assay, although there was a considerable spread in individual values. It is concluded that the various handling procedures of blood or plasma have no significant effect on T and Adione levels.", "PMID": 971533}
{"id": "pubmed23n0032_8559", "title": "Changes in serum and urinary myo-inositol levels in chronic glomerulonephritis.", "content": "Serum and urinary myo-inositol and urinary glucose were estimated by means of gas-liquid chromatography in 54 patients with glomerulonephritis with and without renal failure. myo-Inositol clearance was calculated and an index was formulated which reflected changes in glomerular filtration, tubular reabsorption and catabolism of myo-inositol by the kidney. Serum and urinary myo-inositol levels were increased in glomerulonephritis with a close correlation to the degree of renal failure. In advanced forms of glomerulonephritis, glomerular filtration, tubular reabsorption and catabolism of myo-inositol were shown to be markedly deranged. Evidence obtained showed further that a derangement of tubular reabsorption and catabolism of myo-inositol also accompany milder forms of glomerulonephritis without decreased glomerular filtration. The myo-inositol index value, especially, was increased in patients with signs of disease activity as indicated by a histological examination of the kidney tissue. The index can also be regarded as a highly sensitive test of renal failure. Low grade glucosuria was shown to be frequently associated with glomerulonephritis with renal failure. Evidence was produced which suggested that the tubular reabsorption of myo-inositol was deranged earlier than glucose reabsorption in glomerulonephritis, although they may share a common step in the reabsorption process. The data suggest that the estimation of serum and urinary myo-inositol has advantages in the evaluation of kidney function.", "contents": "Changes in serum and urinary myo-inositol levels in chronic glomerulonephritis. Serum and urinary myo-inositol and urinary glucose were estimated by means of gas-liquid chromatography in 54 patients with glomerulonephritis with and without renal failure. myo-Inositol clearance was calculated and an index was formulated which reflected changes in glomerular filtration, tubular reabsorption and catabolism of myo-inositol by the kidney. Serum and urinary myo-inositol levels were increased in glomerulonephritis with a close correlation to the degree of renal failure. In advanced forms of glomerulonephritis, glomerular filtration, tubular reabsorption and catabolism of myo-inositol were shown to be markedly deranged. Evidence obtained showed further that a derangement of tubular reabsorption and catabolism of myo-inositol also accompany milder forms of glomerulonephritis without decreased glomerular filtration. The myo-inositol index value, especially, was increased in patients with signs of disease activity as indicated by a histological examination of the kidney tissue. The index can also be regarded as a highly sensitive test of renal failure. Low grade glucosuria was shown to be frequently associated with glomerulonephritis with renal failure. Evidence was produced which suggested that the tubular reabsorption of myo-inositol was deranged earlier than glucose reabsorption in glomerulonephritis, although they may share a common step in the reabsorption process. The data suggest that the estimation of serum and urinary myo-inositol has advantages in the evaluation of kidney function.", "PMID": 971534}
{"id": "pubmed23n0032_8560", "title": "D-Glyceric acidemia in a patient with chronic metabolic acidosis.", "content": "A patient is described with glyceric acidemia and glyceric aciduria. The main clinical problems in infancy were severe metabolic acidosis and failure to thrive. The patient needs permanent treatment with bicarbonate. Hyperglycinemia, as described in the first case discovered elsewhere, was not present. The glyceric acid was found to have the D-configuration, as analyzed by capillary gas chromatography of its di-O-acetyl-l-menthyl ester. The abnormality may result from a defect in serine metabolism.", "contents": "D-Glyceric acidemia in a patient with chronic metabolic acidosis. A patient is described with glyceric acidemia and glyceric aciduria. The main clinical problems in infancy were severe metabolic acidosis and failure to thrive. The patient needs permanent treatment with bicarbonate. Hyperglycinemia, as described in the first case discovered elsewhere, was not present. The glyceric acid was found to have the D-configuration, as analyzed by capillary gas chromatography of its di-O-acetyl-l-menthyl ester. The abnormality may result from a defect in serine metabolism.", "PMID": 971536}
{"id": "pubmed23n0032_8561", "title": "Photodynamic modification of proteins in human red blood cell membranes, induced by protoporphyrin.", "content": "Illumination of erythrocytes or erythrocyte membranes with visible light in the presence of protoporphyrin causes photodynamic damage of the cell membrane. This process is reflected a.o. by a mutilated ultrastructure and changes of the physical properties of the membrane proteins. Illumination in the presence of protoporphyrin causes association of membrane proteins, leading to blurring of the protein bands in electropheretograms, disappearance of bands and the appearance of protein aggregates on top of the gels. The formation of large protein aggregates is also indicated by Sephadex gel filtration of the solubilized membrane proteins. In kinetic studies it appeared that spectrin and the bands 2.1, 2.2, 2.3 and 6 are most susceptible and that band 3 is least susceptible to this cross-linking reaction. Experimental results indicate that this cross-linking is caused by direct photooxidation of membrane proteins. Peroxidation of unsaturated fatty acids is not involved in the process. The significance of this process for studies on membrane structure and on photodynamic membrane damage is discussed.", "contents": "Photodynamic modification of proteins in human red blood cell membranes, induced by protoporphyrin. Illumination of erythrocytes or erythrocyte membranes with visible light in the presence of protoporphyrin causes photodynamic damage of the cell membrane. This process is reflected a.o. by a mutilated ultrastructure and changes of the physical properties of the membrane proteins. Illumination in the presence of protoporphyrin causes association of membrane proteins, leading to blurring of the protein bands in electropheretograms, disappearance of bands and the appearance of protein aggregates on top of the gels. The formation of large protein aggregates is also indicated by Sephadex gel filtration of the solubilized membrane proteins. In kinetic studies it appeared that spectrin and the bands 2.1, 2.2, 2.3 and 6 are most susceptible and that band 3 is least susceptible to this cross-linking reaction. Experimental results indicate that this cross-linking is caused by direct photooxidation of membrane proteins. Peroxidation of unsaturated fatty acids is not involved in the process. The significance of this process for studies on membrane structure and on photodynamic membrane damage is discussed.", "PMID": 971537}
{"id": "pubmed23n0032_8562", "title": "The microdetermination of copper in plasma protein fractions.", "content": "Electrophoresis on cellulose acetate membranes and electrothermal atomization for atomic absorption spectrophotometry have been combined to allow the determination of copper in protein fractions separated from only 2 mul volumes of serum. The sensitivity of the method is 5.2 X 10(-11) g of copper for 1% absorption at 324.7 nm, and the precision of replicate analyses at the 1.7-ng level is 0.086 (Relative Standard deviation). The mean recovery of copper from the alpha2-protein fraction of a serum sample was 94%. The method has been applied to a study of the changes in the copper content of the various protein fractions of sera from patients with Menkes' syndrome.", "contents": "The microdetermination of copper in plasma protein fractions. Electrophoresis on cellulose acetate membranes and electrothermal atomization for atomic absorption spectrophotometry have been combined to allow the determination of copper in protein fractions separated from only 2 mul volumes of serum. The sensitivity of the method is 5.2 X 10(-11) g of copper for 1% absorption at 324.7 nm, and the precision of replicate analyses at the 1.7-ng level is 0.086 (Relative Standard deviation). The mean recovery of copper from the alpha2-protein fraction of a serum sample was 94%. The method has been applied to a study of the changes in the copper content of the various protein fractions of sera from patients with Menkes' syndrome.", "PMID": 971538}
{"id": "pubmed23n0032_8563", "title": "A new form of the solubility test for hemoglobin S; results from a survey of 3000 cases.", "content": "A new form of the solubility test for the detection of Hemoglobin S is described. Glass ampoules containing one ml of the test solution ready for use are employed; they can be stored for more than one year at 4 degrees C. 3000 blood samples were analyzed by the solubility test and electrophoresis. The comparison between the two methods showed that the solubility test is highly satisfactory.", "contents": "A new form of the solubility test for hemoglobin S; results from a survey of 3000 cases. A new form of the solubility test for the detection of Hemoglobin S is described. Glass ampoules containing one ml of the test solution ready for use are employed; they can be stored for more than one year at 4 degrees C. 3000 blood samples were analyzed by the solubility test and electrophoresis. The comparison between the two methods showed that the solubility test is highly satisfactory.", "PMID": 971540}
{"id": "pubmed23n0032_8564", "title": "Hypothalamic-pituitary function following successful treatment of intracranial tumours.", "content": "Hypothalamic-pituitary function was examined in seventeen patients treated in the past for extrasellar intracranial tumours. All patients had received a course of external cranial irradiation. Biochemical abnormalities of endocrine function were present in almost all of the patients. In particular, the growth hormone response to insulin induced hypoglycaemic stress was impaired in eleven out of fifteen patients. Preliminary data from a group of seven patients with intracranial tumours prior to radiotherapy are also presented. The possibility that cranial irradiation causes hypothalamic-pituitary dysfunction is discussed. The results suggest that endocrine assessment should be considered in patients who have been treated for intracranial tumours, even in the absence of direct pituitary involvement.", "contents": "Hypothalamic-pituitary function following successful treatment of intracranial tumours. Hypothalamic-pituitary function was examined in seventeen patients treated in the past for extrasellar intracranial tumours. All patients had received a course of external cranial irradiation. Biochemical abnormalities of endocrine function were present in almost all of the patients. In particular, the growth hormone response to insulin induced hypoglycaemic stress was impaired in eleven out of fifteen patients. Preliminary data from a group of seven patients with intracranial tumours prior to radiotherapy are also presented. The possibility that cranial irradiation causes hypothalamic-pituitary dysfunction is discussed. The results suggest that endocrine assessment should be considered in patients who have been treated for intracranial tumours, even in the absence of direct pituitary involvement.", "PMID": 971541}
{"id": "pubmed23n0032_8565", "title": "Cortisol binding capacity and oestrogen concentrations in maternal and cord plasma in pregnancies with normal and anencephalic fetuses.", "content": "The cortisol binding capacity of maternal and cord plasma samples obtained at delivery from fifteen women and their normal infants and from seven women and their anencephalic infants was measured at 4 degrees C by a gel filtration technique. The concentration of oestrogen in these samples was measured by radioimmunoassay. There was no significant difference (t test) between the cortisol binding capacity of peripheral plasma from women with normal infants (1-55 +/- 0-24 mumol/1, mean +/- SD) and from those who delivered anencephalic infants (1-35 +/- 0-30 mumol/1), nor between the cortisol binding capacity of cord plasma from anencephalic infants (0-47 +/- 0-04 mumol/1) and that of normal infants (0-37 +/- 0-10 mumol/1). However, mean oestrogen concentrations in maternal and cord plasma from the pregnancies with an anencephalic fetus were significantly lower (P less than 0-01) than in the corresponding samples from normal pregnancy. It is concluded that oestrogen concentrations in maternal and cord plasma in normal pregnancy at delivery are much greater than those required to account for the increase in plasma cortisol binding capacity. Since plasma cortisol binding capacity in pregnancy with an anencephalic fetus is not diminished, the reduced excretion of corticosteroids relative to normal pregnancy in this condition is unlikely to be due to alterations in cortisol metabolism associated with a lower plasma cortisol binding capacity.", "contents": "Cortisol binding capacity and oestrogen concentrations in maternal and cord plasma in pregnancies with normal and anencephalic fetuses. The cortisol binding capacity of maternal and cord plasma samples obtained at delivery from fifteen women and their normal infants and from seven women and their anencephalic infants was measured at 4 degrees C by a gel filtration technique. The concentration of oestrogen in these samples was measured by radioimmunoassay. There was no significant difference (t test) between the cortisol binding capacity of peripheral plasma from women with normal infants (1-55 +/- 0-24 mumol/1, mean +/- SD) and from those who delivered anencephalic infants (1-35 +/- 0-30 mumol/1), nor between the cortisol binding capacity of cord plasma from anencephalic infants (0-47 +/- 0-04 mumol/1) and that of normal infants (0-37 +/- 0-10 mumol/1). However, mean oestrogen concentrations in maternal and cord plasma from the pregnancies with an anencephalic fetus were significantly lower (P less than 0-01) than in the corresponding samples from normal pregnancy. It is concluded that oestrogen concentrations in maternal and cord plasma in normal pregnancy at delivery are much greater than those required to account for the increase in plasma cortisol binding capacity. Since plasma cortisol binding capacity in pregnancy with an anencephalic fetus is not diminished, the reduced excretion of corticosteroids relative to normal pregnancy in this condition is unlikely to be due to alterations in cortisol metabolism associated with a lower plasma cortisol binding capacity.", "PMID": 971542}
{"id": "pubmed23n0032_8566", "title": "Changes in the pituitary-testicular system with age.", "content": "In order to provide a comprehensive account of pituitary-testicular function in man, 466 subjects, ranging in age from 2 to 101 years, were studied to examine blood levels of the pituitary gonadotrophins (LH and FSH), the sex steroids testosterone and oestradiol, the binding capacity of the sex hormone binding globulin (SHBG), the free testosterone and oestradiol fractions, and the transfer constant for the peripheral conversion of testosterone to oestradiol. The results were compared with clinical indices of testicular size, sexual function and secondary sex hair distribution. Serum LH and FSH were low before puberty, increased in pubertal adolescents to levels somewhat above those of adults and subsequently increased progressively over the age of 40 years. Testosterone levels fell slowly after the age of 40, while there was a slight rise in plasma oestradiol with increasing age. FSH and testosterone showed small seasonal variations in young adult men, the lowest values being seen in winter. SHBG binding capacity was high in two prepubertal boys, fell in adult men, but increased in old age. Free testosterone and oestradiol levels fell in old age. The metabolic clearance rates (MCR) of testosterone and oestradiol also fell in old age, while the conversion of testosterone to oestradiol was increased. Many correlations were observed between various hormonal and clincial measurements. The evidence is consistent with a primary decrease in testicular function over the age of 40 years.", "contents": "Changes in the pituitary-testicular system with age. In order to provide a comprehensive account of pituitary-testicular function in man, 466 subjects, ranging in age from 2 to 101 years, were studied to examine blood levels of the pituitary gonadotrophins (LH and FSH), the sex steroids testosterone and oestradiol, the binding capacity of the sex hormone binding globulin (SHBG), the free testosterone and oestradiol fractions, and the transfer constant for the peripheral conversion of testosterone to oestradiol. The results were compared with clinical indices of testicular size, sexual function and secondary sex hair distribution. Serum LH and FSH were low before puberty, increased in pubertal adolescents to levels somewhat above those of adults and subsequently increased progressively over the age of 40 years. Testosterone levels fell slowly after the age of 40, while there was a slight rise in plasma oestradiol with increasing age. FSH and testosterone showed small seasonal variations in young adult men, the lowest values being seen in winter. SHBG binding capacity was high in two prepubertal boys, fell in adult men, but increased in old age. Free testosterone and oestradiol levels fell in old age. The metabolic clearance rates (MCR) of testosterone and oestradiol also fell in old age, while the conversion of testosterone to oestradiol was increased. Many correlations were observed between various hormonal and clincial measurements. The evidence is consistent with a primary decrease in testicular function over the age of 40 years.", "PMID": 971543}
{"id": "pubmed23n0032_8567", "title": "Hypothalamic hypopituitarism following external radiotherapy for tumours distant from the adenohypophysis.", "content": "Three cases of panhypopituitarism and five of isolated growth hormone deficiency which occurred following previous external irradiation of tumours distant from the adenohypophysis are described. The hypothalamic pituitary region received between 2800 and 12 000 rads in each case, 1-9 years before endocrine deficiency was recognized. Evidence is presented that the site of damage is in the region of the hypothalamus rather than the pituitary gland itself. Individuals treated with X-ray therapy in whom the hypothalamic-pituitary region is exposed to irradiation would appear to be at risk of developing some degree of delayed hypothalamic-pituitary dysfunction.", "contents": "Hypothalamic hypopituitarism following external radiotherapy for tumours distant from the adenohypophysis. Three cases of panhypopituitarism and five of isolated growth hormone deficiency which occurred following previous external irradiation of tumours distant from the adenohypophysis are described. The hypothalamic pituitary region received between 2800 and 12 000 rads in each case, 1-9 years before endocrine deficiency was recognized. Evidence is presented that the site of damage is in the region of the hypothalamus rather than the pituitary gland itself. Individuals treated with X-ray therapy in whom the hypothalamic-pituitary region is exposed to irradiation would appear to be at risk of developing some degree of delayed hypothalamic-pituitary dysfunction.", "PMID": 971544}
{"id": "pubmed23n0032_8568", "title": "A perspective on aldosterone abnormalities.", "content": "Not all the varied clinical disorders in which aldosterone and the mineralocorticoid hormones are involved have been reviewed. Only those disorders in which the mineralocorticoid hormones and their regulatory factors are the principal cause of the biochemical and clinical abnormalities have been examined. These are many and varied. Appreciation of the extent and magnitude of their involvement in the regulation of blood pressure, body fluids, and electrolyte composition continues to grow. The major direct clinical impact of the mineralocorticoid hormones appears to be in two areas: hypertension and potassium homeostasis. Their part in the mosaic of hypertension is established in primary hyperaldosteronism, but they also appear to affect and modify the hypertensive process in primary or essential hypertension. The probe continues. Hypoaldosteronism is more than the rare occurrence associated with Addison's disease. It may be the clue to the presence of nonaldosterone mineralocorticoid excess syndromes, and is obviously of critical importance in an increasing number of patients with chronic renal failure of varied aetiologies.", "contents": "A perspective on aldosterone abnormalities. Not all the varied clinical disorders in which aldosterone and the mineralocorticoid hormones are involved have been reviewed. Only those disorders in which the mineralocorticoid hormones and their regulatory factors are the principal cause of the biochemical and clinical abnormalities have been examined. These are many and varied. Appreciation of the extent and magnitude of their involvement in the regulation of blood pressure, body fluids, and electrolyte composition continues to grow. The major direct clinical impact of the mineralocorticoid hormones appears to be in two areas: hypertension and potassium homeostasis. Their part in the mosaic of hypertension is established in primary hyperaldosteronism, but they also appear to affect and modify the hypertensive process in primary or essential hypertension. The probe continues. Hypoaldosteronism is more than the rare occurrence associated with Addison's disease. It may be the clue to the presence of nonaldosterone mineralocorticoid excess syndromes, and is obviously of critical importance in an increasing number of patients with chronic renal failure of varied aetiologies.", "PMID": 971545}
{"id": "pubmed23n0032_8569", "title": "Thyroid suppression tests during drug treatment of hyperthyroidism.", "content": "In fifteen hyperthyroid patients treated for 5 or more consecutive years with propylthiouracil four or more yearly prognostic 'suppression' tests (Cassidy & VanderLaan, 1960) were done. In nine of the fifteen patients there was a progressive diminution over a period of 3-6 years in the values of these suppressed uptakes to 20% or below. Eight of these nine patients have remained euthyroid off therapy for 1-8 years (average 3-1 years). None have become hypothyroid. The data suggest a gradual continuing change in thyroid function over the course of years in some hyperthyroid patients on antithyroid therapy. This may be a long-term effect of the drug and may have prognostic significance.", "contents": "Thyroid suppression tests during drug treatment of hyperthyroidism. In fifteen hyperthyroid patients treated for 5 or more consecutive years with propylthiouracil four or more yearly prognostic 'suppression' tests (Cassidy & VanderLaan, 1960) were done. In nine of the fifteen patients there was a progressive diminution over a period of 3-6 years in the values of these suppressed uptakes to 20% or below. Eight of these nine patients have remained euthyroid off therapy for 1-8 years (average 3-1 years). None have become hypothyroid. The data suggest a gradual continuing change in thyroid function over the course of years in some hyperthyroid patients on antithyroid therapy. This may be a long-term effect of the drug and may have prognostic significance.", "PMID": 971547}
{"id": "pubmed23n0032_8570", "title": "Effect of dopamine infusion on plasma levels of growth hormone in normal subjects and in agromegalic patients.", "content": "In ten normal subjects and in twenty acromegalic patients plasma levels of growth hormone were studied after administration of L-dopa and a dopamine infusion. In the ten normal subjects L-dopa, but not dopamine, increased plasma levels of GH. In the acromegalic patients both L-dopa and dopamine were followed by an inhibition of GH release. Since dopamine does not cross the blood-brain barrier, these results, although not excluding a site of action at median eminence level, support the thesis that the inhibitory effect of dopaminergic stimulation on GH release in acromegaly possibly involves receptors present on GH-secreting cells.", "contents": "Effect of dopamine infusion on plasma levels of growth hormone in normal subjects and in agromegalic patients. In ten normal subjects and in twenty acromegalic patients plasma levels of growth hormone were studied after administration of L-dopa and a dopamine infusion. In the ten normal subjects L-dopa, but not dopamine, increased plasma levels of GH. In the acromegalic patients both L-dopa and dopamine were followed by an inhibition of GH release. Since dopamine does not cross the blood-brain barrier, these results, although not excluding a site of action at median eminence level, support the thesis that the inhibitory effect of dopaminergic stimulation on GH release in acromegaly possibly involves receptors present on GH-secreting cells.", "PMID": 971548}
{"id": "pubmed23n0032_8571", "title": "Effects of quinidine on blood flow rate and developed tension in blood-perfused canine papillary muscle.", "content": "1. The effects of quinidine on blood flow rate and developed tension were studied in isolated, blood-perfused papillary muscle preparations of the dog. Drugs were injected into the anterior septal artery. 2. Quinidine caused a dose-related increase in blood flow rate; the mean dose producing a 100% increase in blood flow rate was about 0-3 mg. 3. Quinidine in doses of 0-01-0-1 mg produced a positive inotropic response. With 0-3--1 mg of quinidine the positive inotropic response was preceded by a transient negative inotropic response. With 3 mg there was a monophasic negative inotropic response, the developed tension being reduced by about 40% of control. 4. Propranolol had no statistically significant effects on the responses of the blood flow rate and developed tension caused by quinidine. 5. These results indicate that quinidine has an action on coronary vessels in lower doses than on the myocardium, and that in low doses it has a positive inotropic action rather than the well-known negative inotrophic action exerted with higher doses.", "contents": "Effects of quinidine on blood flow rate and developed tension in blood-perfused canine papillary muscle. 1. The effects of quinidine on blood flow rate and developed tension were studied in isolated, blood-perfused papillary muscle preparations of the dog. Drugs were injected into the anterior septal artery. 2. Quinidine caused a dose-related increase in blood flow rate; the mean dose producing a 100% increase in blood flow rate was about 0-3 mg. 3. Quinidine in doses of 0-01-0-1 mg produced a positive inotropic response. With 0-3--1 mg of quinidine the positive inotropic response was preceded by a transient negative inotropic response. With 3 mg there was a monophasic negative inotropic response, the developed tension being reduced by about 40% of control. 4. Propranolol had no statistically significant effects on the responses of the blood flow rate and developed tension caused by quinidine. 5. These results indicate that quinidine has an action on coronary vessels in lower doses than on the myocardium, and that in low doses it has a positive inotropic action rather than the well-known negative inotrophic action exerted with higher doses.", "PMID": 971549}
{"id": "pubmed23n0032_8572", "title": "The hepatic transport of taurocholic acid in the rat: development of a mathematical model.", "content": "1. The transport of taurocholic acid from portal blood to bile was studied in the anaesthetized rat by injecting a radiolabelled pulse of bile acid. 2. The transport process was very rapid, 50% of the dose being secreted within 5 min, and the total dose within 15 min. 3. The transport process had a large capacity. The secretory profile was little modified by a 500-fold increase in the injected dose. 4. The transport process was modelled with both analogue and digital computers. The simplest model which fitted the data comprised rapid uptake from portal blood, slow transport across the cell and rapid secretion into bile. The digital computer simulation suggested that this model is not unique, but will require further testing.", "contents": "The hepatic transport of taurocholic acid in the rat: development of a mathematical model. 1. The transport of taurocholic acid from portal blood to bile was studied in the anaesthetized rat by injecting a radiolabelled pulse of bile acid. 2. The transport process was very rapid, 50% of the dose being secreted within 5 min, and the total dose within 15 min. 3. The transport process had a large capacity. The secretory profile was little modified by a 500-fold increase in the injected dose. 4. The transport process was modelled with both analogue and digital computers. The simplest model which fitted the data comprised rapid uptake from portal blood, slow transport across the cell and rapid secretion into bile. The digital computer simulation suggested that this model is not unique, but will require further testing.", "PMID": 971550}
{"id": "pubmed23n0032_8573", "title": "Release of renin from glomeruli isolated from rat kidney.", "content": "1. Glomeruli free from renal tubules and cell debris were isolated in large numbers from rat kidney cortex by a rapid, simple process of graded sieving on stainless steel meshes. 2. Glomeruli were viable, had fragments of the arterioles attached and contained renin. 3. Glomeruli, when incubated, released renin into the medium. Using a super-fusion technique this was shown to be time dependent until a basal secretion level was achieved. 4. Release of renin was directly proportional to the number of glomeruli and could be stimulated by isoprenaline, adrenaline and noradrenaline in order of the potency of their action on beta-adrenoreceptors. 5. Isolated glomeruli were therefore established as a useful model system for studying the release of renin by direct influences on the juxtaglomerular cells where haemodynamic, tubular and extrarenal influences are removed.", "contents": "Release of renin from glomeruli isolated from rat kidney. 1. Glomeruli free from renal tubules and cell debris were isolated in large numbers from rat kidney cortex by a rapid, simple process of graded sieving on stainless steel meshes. 2. Glomeruli were viable, had fragments of the arterioles attached and contained renin. 3. Glomeruli, when incubated, released renin into the medium. Using a super-fusion technique this was shown to be time dependent until a basal secretion level was achieved. 4. Release of renin was directly proportional to the number of glomeruli and could be stimulated by isoprenaline, adrenaline and noradrenaline in order of the potency of their action on beta-adrenoreceptors. 5. Isolated glomeruli were therefore established as a useful model system for studying the release of renin by direct influences on the juxtaglomerular cells where haemodynamic, tubular and extrarenal influences are removed.", "PMID": 971551}
{"id": "pubmed23n0032_8574", "title": "Effects of glucagon on pancreatic secretion in the dog.", "content": "1. The effects of glucagon on the secretion of pancreatic juice were investigated using blood-perfused canine pancreas preparations. 2. Intravenous administration of glucagon (3-30 mug/kg) to the donor dog elicited a dose-dependent increase in pancreatic secretion. Intra-arterial administration of glucagon (10-100 mug) into the perfused pancreas also elicited increased secretion. 3. There were slight increases in amylase concentration of the pancreatic juice with the largest doses of glucagon given by either route. 4. Glucagon-induced secretion was not modified by treatment with phentolamine, propranolol, atropine, guanethidine, tetradotoxin, haloperidol, prostaglandin F2alpha or calcitonin. 5. The results suggest that glucagon acts directly on the exocrine cells of the canine pancreas.", "contents": "Effects of glucagon on pancreatic secretion in the dog. 1. The effects of glucagon on the secretion of pancreatic juice were investigated using blood-perfused canine pancreas preparations. 2. Intravenous administration of glucagon (3-30 mug/kg) to the donor dog elicited a dose-dependent increase in pancreatic secretion. Intra-arterial administration of glucagon (10-100 mug) into the perfused pancreas also elicited increased secretion. 3. There were slight increases in amylase concentration of the pancreatic juice with the largest doses of glucagon given by either route. 4. Glucagon-induced secretion was not modified by treatment with phentolamine, propranolol, atropine, guanethidine, tetradotoxin, haloperidol, prostaglandin F2alpha or calcitonin. 5. The results suggest that glucagon acts directly on the exocrine cells of the canine pancreas.", "PMID": 971553}
{"id": "pubmed23n0032_8575", "title": "Comparison of the effects of (--)-isoprenaline, orciprenaline terbutaline, and Me506 on heart rate, soleus muscle contractility and pulmonary resistance of anaesthetized cats.", "content": "1. Three resorcinol derivatives with N-isopropyl (orciprenaline), N-t-butyl (terbutaline) and N-p-hydroxypheny-t-butyl (Me506) amine substituents have been compared with (--)-isoprenaline for their ability to produce beta-receptor mediated reductions in serotonin-induced increases in pulmonary resistance, decreases in soleus muscle contractility and increases in heart rate in anaesthetized cats. 2. For all parameters studied the four compounds produced similar maximal responses and dose-response curves were close to parallel. From the graphs doses of the compounds producing 50% of the maximal response (ED50) were interpolated, and from these dose-ratios with respect to (--)-isoprenaline [drug ED50:(--)-isoprenaline ED50] were calculated on a molar basis. 3. Increasing the size of the amine substituent from N-isopropyl to N-t-butyl led to an increase in beta-receptor stimulant activity in bronchial and skeletal muscle, but not in the heart. The change from N-t-butyl to N-p-hydroxyphenyl-t-butyl did not further affect stimulant activity in any of the parameters studied. 4. Calculation of selectivity ratios [molar dose-ratio (heart): molar dose-ratio (pulmonary resistance)] showed that orciprenaline was non-selective, and that terbutaline and Me506 showed a similar degree of selectivity for beta2- as opposed to beta1-receptor mediated actions.", "contents": "Comparison of the effects of (--)-isoprenaline, orciprenaline terbutaline, and Me506 on heart rate, soleus muscle contractility and pulmonary resistance of anaesthetized cats. 1. Three resorcinol derivatives with N-isopropyl (orciprenaline), N-t-butyl (terbutaline) and N-p-hydroxypheny-t-butyl (Me506) amine substituents have been compared with (--)-isoprenaline for their ability to produce beta-receptor mediated reductions in serotonin-induced increases in pulmonary resistance, decreases in soleus muscle contractility and increases in heart rate in anaesthetized cats. 2. For all parameters studied the four compounds produced similar maximal responses and dose-response curves were close to parallel. From the graphs doses of the compounds producing 50% of the maximal response (ED50) were interpolated, and from these dose-ratios with respect to (--)-isoprenaline [drug ED50:(--)-isoprenaline ED50] were calculated on a molar basis. 3. Increasing the size of the amine substituent from N-isopropyl to N-t-butyl led to an increase in beta-receptor stimulant activity in bronchial and skeletal muscle, but not in the heart. The change from N-t-butyl to N-p-hydroxyphenyl-t-butyl did not further affect stimulant activity in any of the parameters studied. 4. Calculation of selectivity ratios [molar dose-ratio (heart): molar dose-ratio (pulmonary resistance)] showed that orciprenaline was non-selective, and that terbutaline and Me506 showed a similar degree of selectivity for beta2- as opposed to beta1-receptor mediated actions.", "PMID": 971552}
{"id": "pubmed23n0032_8576", "title": "Catechol-O-methyltransferase activity in erythrocytes of children with autism.", "content": "1. The activity of the enzyme catechol-O-methyltransferase (COMT) was determined in the erythrocytes of normal and autistic children. 2. There was no difference in enzyme activity between the two groups, although in both the normal and autistic females the erythrocyte COMT activity showed considerable within-group variation. 3. When the erythrocyte homogenates were divided into particulate and soluble fractions, some differences were observed. The COMT activity in the soluble fraction was less in erythrocytes from autistic males than in those from control males, whereas that in autistic females was higher than in those from control females.", "contents": "Catechol-O-methyltransferase activity in erythrocytes of children with autism. 1. The activity of the enzyme catechol-O-methyltransferase (COMT) was determined in the erythrocytes of normal and autistic children. 2. There was no difference in enzyme activity between the two groups, although in both the normal and autistic females the erythrocyte COMT activity showed considerable within-group variation. 3. When the erythrocyte homogenates were divided into particulate and soluble fractions, some differences were observed. The COMT activity in the soluble fraction was less in erythrocytes from autistic males than in those from control males, whereas that in autistic females was higher than in those from control females.", "PMID": 971554}
{"id": "pubmed23n0032_8577", "title": "Role of the renin-angiotensin system in hypertension produced by unilateral renal artery constriction in the rat.", "content": "1. Conscious rats which had undergone unilateral renal artery constriction were infused for 1 h with a specific antagonist of angiotensin II, 1-Sar-8-Ala-angiotensin II (P-113). 2. There was a highly significant correlation between the change in blood pressure induced by P-113 and the pre-infusion plasma renin concentration (PRC), regardless of initial blood pressure or the duration of stenosis. However, the blood pressure fall was not significantly greater in nineteen hypertensive rats than in eleven which remained normotensive. P-113 did not abolish the hypertension. 3. The extent to which angiotensin II supports blood pressure in rats with renal artery constriction is directly related to the PRC.", "contents": "Role of the renin-angiotensin system in hypertension produced by unilateral renal artery constriction in the rat. 1. Conscious rats which had undergone unilateral renal artery constriction were infused for 1 h with a specific antagonist of angiotensin II, 1-Sar-8-Ala-angiotensin II (P-113). 2. There was a highly significant correlation between the change in blood pressure induced by P-113 and the pre-infusion plasma renin concentration (PRC), regardless of initial blood pressure or the duration of stenosis. However, the blood pressure fall was not significantly greater in nineteen hypertensive rats than in eleven which remained normotensive. P-113 did not abolish the hypertension. 3. The extent to which angiotensin II supports blood pressure in rats with renal artery constriction is directly related to the PRC.", "PMID": 971555}
{"id": "pubmed23n0032_8578", "title": "The fate of the orally administered bile acid sequestrant, polidexide, in humans.", "content": "1. The metabolic fate of the insoluble bile acid sequestrant polidexide, (poly-[2-(diethylamino)ethyl] polyglycerylenedextran hydrochloride), was studied in four adult humans following the oral administration of the 14C-labelled substance. 2. The mean cumulative recovery of 14C in faeces was 95-3% (s.e.m. = 1-1) of the administered dose, while mean cumulative recovery in urine was 0-37% (s.e.m. = 0-13) of the oral dose. 3. Only background levels of radioactivity were detectable in plasma samples taken 1-3 days after administration of tracer. 4. The findings suggested that polidexide was not absorbed from the gastrointestinal in man to any significant degree.", "contents": "The fate of the orally administered bile acid sequestrant, polidexide, in humans. 1. The metabolic fate of the insoluble bile acid sequestrant polidexide, (poly-[2-(diethylamino)ethyl] polyglycerylenedextran hydrochloride), was studied in four adult humans following the oral administration of the 14C-labelled substance. 2. The mean cumulative recovery of 14C in faeces was 95-3% (s.e.m. = 1-1) of the administered dose, while mean cumulative recovery in urine was 0-37% (s.e.m. = 0-13) of the oral dose. 3. Only background levels of radioactivity were detectable in plasma samples taken 1-3 days after administration of tracer. 4. The findings suggested that polidexide was not absorbed from the gastrointestinal in man to any significant degree.", "PMID": 971556}
{"id": "pubmed23n0032_8579", "title": "Gas exchange during exercise in healthy people. I. The physiological dead-space volume.", "content": "1. Physiological dead-space volume (V D) was measured in twenty-four healthy men and women aged from 20 to 71 years, at rest and at two rates of work on a treadmill, whilst breathing air and breathing oxygen. 2. The effect of correction of arterial carbon dioxide tension (Pa,CO2) to pulmonary capillary temperature on the resulting value for V D was investigated. We find that the effect is substantial and that a correction should be made. 3. Equations have been derived for the prediction of normal V D during exercise. The best prediction was given by a regression on height, age, carbon dioxide output, ventilation and respiratory frequency, with an upper 95% confidence limit of +81 ml.", "contents": "Gas exchange during exercise in healthy people. I. The physiological dead-space volume. 1. Physiological dead-space volume (V D) was measured in twenty-four healthy men and women aged from 20 to 71 years, at rest and at two rates of work on a treadmill, whilst breathing air and breathing oxygen. 2. The effect of correction of arterial carbon dioxide tension (Pa,CO2) to pulmonary capillary temperature on the resulting value for V D was investigated. We find that the effect is substantial and that a correction should be made. 3. Equations have been derived for the prediction of normal V D during exercise. The best prediction was given by a regression on height, age, carbon dioxide output, ventilation and respiratory frequency, with an upper 95% confidence limit of +81 ml.", "PMID": 971573}
{"id": "pubmed23n0032_8580", "title": "Gas exchange during exercise in healthy people II. Venous admixture.", "content": "1. Venous admixture/cardiac output ratio (Qva/Qt) has been measured in twenty-four healthy volunteer subjects of both sexes aged 20-71 years, at rest and during the steady state of treadmill exercise at two rates of work, and breathing air and breathing oxygen. 2. With oxygen breathing, Qva/Qt was considerably less during exercise than during the time subjects were taking either normal or deep breaths of oxygen at rest, and did not significantly increase with the intensity of exercise. It is postulated that the increase in ventilation during exercise opens most or all of those alveoli which, during oxygen breathing at rest, close because of critically low ventilation/perfusion (V/Q) ratios. 3. With air breathing, Qva/Qt fell from rest to exercise (especially in older subjects), presumably due to improved ventilation of alveoli at the lung bases. With an increase in work rate Qva/Qt increased in all age groups. This increase was not due to increase in the shunt fraction (Qs/Qt), nor to limitation of diffusing capacity; it arose from an increase in V/Q variance. 4. Equations have been derived for the prediction of normal Qva/Qt during exercise, with or without correction for the effects of increasing pulmonary capillary temperature. These effects do not materially influence the accuracy of prediction, but may be relevant to some of the interpretations. In particular, they provide a further indication that Qs/Qt probably cannot be measured by breathing oxygen at rest, even in deep breathing.", "contents": "Gas exchange during exercise in healthy people II. Venous admixture. 1. Venous admixture/cardiac output ratio (Qva/Qt) has been measured in twenty-four healthy volunteer subjects of both sexes aged 20-71 years, at rest and during the steady state of treadmill exercise at two rates of work, and breathing air and breathing oxygen. 2. With oxygen breathing, Qva/Qt was considerably less during exercise than during the time subjects were taking either normal or deep breaths of oxygen at rest, and did not significantly increase with the intensity of exercise. It is postulated that the increase in ventilation during exercise opens most or all of those alveoli which, during oxygen breathing at rest, close because of critically low ventilation/perfusion (V/Q) ratios. 3. With air breathing, Qva/Qt fell from rest to exercise (especially in older subjects), presumably due to improved ventilation of alveoli at the lung bases. With an increase in work rate Qva/Qt increased in all age groups. This increase was not due to increase in the shunt fraction (Qs/Qt), nor to limitation of diffusing capacity; it arose from an increase in V/Q variance. 4. Equations have been derived for the prediction of normal Qva/Qt during exercise, with or without correction for the effects of increasing pulmonary capillary temperature. These effects do not materially influence the accuracy of prediction, but may be relevant to some of the interpretations. In particular, they provide a further indication that Qs/Qt probably cannot be measured by breathing oxygen at rest, even in deep breathing.", "PMID": 971574}
{"id": "pubmed23n0032_8581", "title": "Effects of parathyroid hormone on sodium and calcium transport in the dog nephron.", "content": "1. The effect of purified bovine parathyroid hormone on renal tubular reabsorption of sodium and calcium has been studied by micropuncture in intact and recently thyroparathyroidectomized dogs. 2. Parathyroid hormone increased the rejection of sodium and calcium proportionately at the late proximal tubule in both intact and operated dogs. 3. In both groups of dogs, there was increased delivery of sodium and calcium to the distal tubule after the hormone. However, the Ca/Na ratio decreased, suggesting some selective enhancement of calcium reabsorption before the superficial distal puncture site. 4. In the final urine, the Ca/Na ratio decreased highly significantly in both groups of dogs, indicating a further selective effect of parathyroid hormone on calcium reabsorption in or beyond the distal convoluted tubule.", "contents": "Effects of parathyroid hormone on sodium and calcium transport in the dog nephron. 1. The effect of purified bovine parathyroid hormone on renal tubular reabsorption of sodium and calcium has been studied by micropuncture in intact and recently thyroparathyroidectomized dogs. 2. Parathyroid hormone increased the rejection of sodium and calcium proportionately at the late proximal tubule in both intact and operated dogs. 3. In both groups of dogs, there was increased delivery of sodium and calcium to the distal tubule after the hormone. However, the Ca/Na ratio decreased, suggesting some selective enhancement of calcium reabsorption before the superficial distal puncture site. 4. In the final urine, the Ca/Na ratio decreased highly significantly in both groups of dogs, indicating a further selective effect of parathyroid hormone on calcium reabsorption in or beyond the distal convoluted tubule.", "PMID": 971575}
{"id": "pubmed23n0032_8582", "title": "Chloride depletion and hypochloraemia as a cause of renal sodium and water loss in the rat.", "content": "1. To study the effects of chloride depletion, without sodium depletion or change in plasma tonicity, on renal excretion of sodium and water, a single exchange peritoneal dialysis was performed in rats against a solution of glucose (15 g/1) containing either NaCl (150 mmol/l, control) or NaHCO3 (150 mmol/l, experimental); KHCO3 (4mmol/l)was added to both solutions. All rats were prepared before dialysis by a low NaCl diet for 10 days. 2. Peritoneal dialysis against NAHCO3 consistently produced a negative sodium and water balance compared with dialysis against NaCl. Despite this, subsequent electrolyte balance for 3 days showed that chloride-depleted rats excreted significantly more sodium and water and had a reduced urinary osmolality as compared with control animals. Increased sodium and water loss were unexplained by osmotic or bicarbonate diuresis. Kaliuresis was seen in the chloride-depleted rats but muscle potassium was not significantly depressed. 3. With sodium and water loss and continued renal chloride conservation, plasma chloride rose on the average from 88 mmol/l after dialysis against NaHCO3 to 100 mmol/l (control 104 mmol/l) at 72 h. Concomitant with this increase in plasma [C1-], on the third day after dialysis, during hydropenia, urinary osmolality and papillary [Na+] were not different from control cencentrations. 4. It is postulated that chloride depletion and/or hypochloraemia leads to diminished chloride transport in the loop of Henle and that this causes reduced sodium transport into the medulla, impaired concentration ability and inappropriate urinary sodium loss.", "contents": "Chloride depletion and hypochloraemia as a cause of renal sodium and water loss in the rat. 1. To study the effects of chloride depletion, without sodium depletion or change in plasma tonicity, on renal excretion of sodium and water, a single exchange peritoneal dialysis was performed in rats against a solution of glucose (15 g/1) containing either NaCl (150 mmol/l, control) or NaHCO3 (150 mmol/l, experimental); KHCO3 (4mmol/l)was added to both solutions. All rats were prepared before dialysis by a low NaCl diet for 10 days. 2. Peritoneal dialysis against NAHCO3 consistently produced a negative sodium and water balance compared with dialysis against NaCl. Despite this, subsequent electrolyte balance for 3 days showed that chloride-depleted rats excreted significantly more sodium and water and had a reduced urinary osmolality as compared with control animals. Increased sodium and water loss were unexplained by osmotic or bicarbonate diuresis. Kaliuresis was seen in the chloride-depleted rats but muscle potassium was not significantly depressed. 3. With sodium and water loss and continued renal chloride conservation, plasma chloride rose on the average from 88 mmol/l after dialysis against NaHCO3 to 100 mmol/l (control 104 mmol/l) at 72 h. Concomitant with this increase in plasma [C1-], on the third day after dialysis, during hydropenia, urinary osmolality and papillary [Na+] were not different from control cencentrations. 4. It is postulated that chloride depletion and/or hypochloraemia leads to diminished chloride transport in the loop of Henle and that this causes reduced sodium transport into the medulla, impaired concentration ability and inappropriate urinary sodium loss.", "PMID": 971576}
{"id": "pubmed23n0032_8583", "title": "Urinary excretion of peptides and of hydroxyproline in Wilson's disease.", "content": "1. A study has been made of peptide excretion in twenty cases of Wilson's disease, ligand-exchange column chromatography being used to separate peptides from free amino acids. Previous reports of excess of peptide output in the disease were confirmed and the excess was shown to be highly significant statistically. 2. A considerable fraction of the excess of peptide output was shown to consist of hydroxyproline-containing peptides derived from collagen degradation. 3. The method of rank correlation showed that the difference both in free amino acid and peptide-bound amino acid output in cases of Wilson's disease and in control subjects was mainly quantitative; the pattern of amino acid excretion was qualitatively similar in both groups. 4. Evidence is presented that the increase copper output in the urine in the disease is not secondary to peptiduria.", "contents": "Urinary excretion of peptides and of hydroxyproline in Wilson's disease. 1. A study has been made of peptide excretion in twenty cases of Wilson's disease, ligand-exchange column chromatography being used to separate peptides from free amino acids. Previous reports of excess of peptide output in the disease were confirmed and the excess was shown to be highly significant statistically. 2. A considerable fraction of the excess of peptide output was shown to consist of hydroxyproline-containing peptides derived from collagen degradation. 3. The method of rank correlation showed that the difference both in free amino acid and peptide-bound amino acid output in cases of Wilson's disease and in control subjects was mainly quantitative; the pattern of amino acid excretion was qualitatively similar in both groups. 4. Evidence is presented that the increase copper output in the urine in the disease is not secondary to peptiduria.", "PMID": 971577}
{"id": "pubmed23n0032_8584", "title": "A study in vivo of peritubular oncotic pressure and proximal tubular reabsorption in the rat.", "content": "1. Peritubular capillary microperfusion was used to examine the effects of protein-free and hyperoncotic homologous plasma on fluid reabsorption by proximal convoluted tubules in the hydropenic rat. 3H-labelled p-aminohippurate was added to perfusates for the purpose of estimating the extent to which tubules under study were bathed by the perfusates. [14C]Mannitol was added to perfusates in order to detect contamination of collected tubular fluid by perfusates. 2. Hydrostatic pressures were monitored in the peritubular microvasculature and adjacent proximal tubules during perfusion. Evidence for secretion of p-aminohippurate from perfusate into tubules under study was determined by collecting tubular fluid from both early and late puncture site. Fractional and absolute reabsorption were not affected by either the protein-free or the hyperoncotic plasma. 3. When acetazolamide was added to the perfusate both fractional and absolute reabsorptive rates decreased by an average of 36%, indicating that the techniques were capable of detecting a decrease in proximal tubular reabsorption. 4. It is concluded that under the conditions of this study changes in peritubular capillary protein concentrations have no detectable effect on the rate of proximal convoluted tubule fluid reabsorption.", "contents": "A study in vivo of peritubular oncotic pressure and proximal tubular reabsorption in the rat. 1. Peritubular capillary microperfusion was used to examine the effects of protein-free and hyperoncotic homologous plasma on fluid reabsorption by proximal convoluted tubules in the hydropenic rat. 3H-labelled p-aminohippurate was added to perfusates for the purpose of estimating the extent to which tubules under study were bathed by the perfusates. [14C]Mannitol was added to perfusates in order to detect contamination of collected tubular fluid by perfusates. 2. Hydrostatic pressures were monitored in the peritubular microvasculature and adjacent proximal tubules during perfusion. Evidence for secretion of p-aminohippurate from perfusate into tubules under study was determined by collecting tubular fluid from both early and late puncture site. Fractional and absolute reabsorption were not affected by either the protein-free or the hyperoncotic plasma. 3. When acetazolamide was added to the perfusate both fractional and absolute reabsorptive rates decreased by an average of 36%, indicating that the techniques were capable of detecting a decrease in proximal tubular reabsorption. 4. It is concluded that under the conditions of this study changes in peritubular capillary protein concentrations have no detectable effect on the rate of proximal convoluted tubule fluid reabsorption.", "PMID": 971578}
{"id": "pubmed23n0032_8585", "title": "Elimination of cholesterol in hyperlipoproteinaemia.", "content": "1. Cholesterol intake (about 0-25 mmol/day) and the faecal excretion of cholesterol, coprostanol and coprostanone were determined in normolipidaemic control subjects and hyperlipidaemic patients, whose bile acid kinetics had been previously studied. 2. The combined excretion of the neutral steroids (excluding plant sterol and plant sterol metabolites) averaged 1-07 +/- 0-13 (+/-SEM)mmol/day in the control subjects (n=14). The corresponding values in patients with hyperlipoproteinaemia type IIa (n=19), IIb (n=12) and IV (n=23) were 0-86 +/- 0-10, 0-93 +/- 0-11 and 1-48 +/- 0-17 mmol/day respectively. 3. The mean values for the net steroid \"balance\", defined as the combined amount of bile acid synthesized (determined by an isotope-dilution technique) and the faecal excretion of neutral steroids minus cholesterol intake, were 1-83 +/- 0-22 mmol/day in the control subjects and 1-60 +/- 0-15, 1-81 +/- 0-19 and 3-53 +/- 0-23 mmol/day in patients with type IIa, IIb and IV lipoprotein patterns respectively. 4. No significant correlations between net steroid \"balance\" and sex, age, serum lipid concentrations, body weight or body surface area were found in any of the groups of subjects. 5. It is concluded that patients with type II hyperlipoproteinaemia eliminate cholesterol as bile acids and neutral faecal steroids normally. The type IV lipoprotein pattern is associated with increased bile acid synthesis and/or elevated faecal excretion of neutral steroids, so that the net steroid \"balance\" is usually above the normal limit.", "contents": "Elimination of cholesterol in hyperlipoproteinaemia. 1. Cholesterol intake (about 0-25 mmol/day) and the faecal excretion of cholesterol, coprostanol and coprostanone were determined in normolipidaemic control subjects and hyperlipidaemic patients, whose bile acid kinetics had been previously studied. 2. The combined excretion of the neutral steroids (excluding plant sterol and plant sterol metabolites) averaged 1-07 +/- 0-13 (+/-SEM)mmol/day in the control subjects (n=14). The corresponding values in patients with hyperlipoproteinaemia type IIa (n=19), IIb (n=12) and IV (n=23) were 0-86 +/- 0-10, 0-93 +/- 0-11 and 1-48 +/- 0-17 mmol/day respectively. 3. The mean values for the net steroid \"balance\", defined as the combined amount of bile acid synthesized (determined by an isotope-dilution technique) and the faecal excretion of neutral steroids minus cholesterol intake, were 1-83 +/- 0-22 mmol/day in the control subjects and 1-60 +/- 0-15, 1-81 +/- 0-19 and 3-53 +/- 0-23 mmol/day in patients with type IIa, IIb and IV lipoprotein patterns respectively. 4. No significant correlations between net steroid \"balance\" and sex, age, serum lipid concentrations, body weight or body surface area were found in any of the groups of subjects. 5. It is concluded that patients with type II hyperlipoproteinaemia eliminate cholesterol as bile acids and neutral faecal steroids normally. The type IV lipoprotein pattern is associated with increased bile acid synthesis and/or elevated faecal excretion of neutral steroids, so that the net steroid \"balance\" is usually above the normal limit.", "PMID": 971579}
{"id": "pubmed23n0032_8586", "title": "A study of changes in whole-body calcium, phosphorus, sodium and nitrogen by neutron activation analysis in vivo in rats on a calcium-deficient diet.", "content": "1. A method of measuring changes in the total body content of calcium, phosphorus, nitrogen and sodium in rats by activation analysis in vivo is described. 2. The change in the body content of the elements has been measured in rats on a calcium-deficient diet and in control animals, the body nitrogen being used to represent lean body mass for normalization. 3. There were siginificant differences in Ca/N and P/N but not in Ca/P ratios between the animals on a deficient diet and control animals at the end of the dietary period.", "contents": "A study of changes in whole-body calcium, phosphorus, sodium and nitrogen by neutron activation analysis in vivo in rats on a calcium-deficient diet. 1. A method of measuring changes in the total body content of calcium, phosphorus, nitrogen and sodium in rats by activation analysis in vivo is described. 2. The change in the body content of the elements has been measured in rats on a calcium-deficient diet and in control animals, the body nitrogen being used to represent lean body mass for normalization. 3. There were siginificant differences in Ca/N and P/N but not in Ca/P ratios between the animals on a deficient diet and control animals at the end of the dietary period.", "PMID": 971580}
{"id": "pubmed23n0032_8587", "title": "The effect of aluminium hydroxide orally on calcium, phosphorus and aluminium metabolism in normal subjects.", "content": "1. Five normal subjects were given 100 ml of aluminium hydroxide gel per day for 28 days. 2. The phosphorus balance became more positive in one subject, less negative in two and changed from negative to positive in the other two subjects. This was accompanied by a rise in the concentration of the fasting morning plasma phosphorus. Calcium balance did not change. 3. The normal subjects absorbed  0-3-3-6 mmol of aluminium/day, which is significantly less than that absorbed by five patients with chronic renal failure, three of whom were studied before, and two after, the observations on the normal subjects had been completed. 4. In a further five normal subjects on 100 ml of aluminium hydroxide gel/day the 08.00 hours concentration of plasma phosphorus did not fall, though there was a fall at 11.00, 14.00 and 17.00 hours.", "contents": "The effect of aluminium hydroxide orally on calcium, phosphorus and aluminium metabolism in normal subjects. 1. Five normal subjects were given 100 ml of aluminium hydroxide gel per day for 28 days. 2. The phosphorus balance became more positive in one subject, less negative in two and changed from negative to positive in the other two subjects. This was accompanied by a rise in the concentration of the fasting morning plasma phosphorus. Calcium balance did not change. 3. The normal subjects absorbed  0-3-3-6 mmol of aluminium/day, which is significantly less than that absorbed by five patients with chronic renal failure, three of whom were studied before, and two after, the observations on the normal subjects had been completed. 4. In a further five normal subjects on 100 ml of aluminium hydroxide gel/day the 08.00 hours concentration of plasma phosphorus did not fall, though there was a fall at 11.00, 14.00 and 17.00 hours.", "PMID": 971581}
{"id": "pubmed23n0032_8588", "title": "The effect of cryogenic storage on human erythrocyte membrane proteins as determined by polyacrylamide-gel electrophoresis.", "content": "A study was conducted to determine the effects of freezing on the major membrane proteins of isolated human erythrocyte membranes. Membranes in low or normal ionic strength medium were frozen at slow or fast freezing rates. The membrane protein composition and elution of proteins from the membranes were studied utilizing polyacrylamide-gel electrophoresis in a sodium dodecyl sulfate or an acetic acid-urea-phenol solvent system. Neither a change in the composition of the membrane proteins nor any elution of membrane protein during freezing and thawing was observed. The data indicate that any human erythrocyte membrane damage during freezing and thawing was not related to a change in major membrane protein composition. Human red cell membranes were stable at --80 or --196degreesC in the absence of a cryoprotective agent.", "contents": "The effect of cryogenic storage on human erythrocyte membrane proteins as determined by polyacrylamide-gel electrophoresis. A study was conducted to determine the effects of freezing on the major membrane proteins of isolated human erythrocyte membranes. Membranes in low or normal ionic strength medium were frozen at slow or fast freezing rates. The membrane protein composition and elution of proteins from the membranes were studied utilizing polyacrylamide-gel electrophoresis in a sodium dodecyl sulfate or an acetic acid-urea-phenol solvent system. Neither a change in the composition of the membrane proteins nor any elution of membrane protein during freezing and thawing was observed. The data indicate that any human erythrocyte membrane damage during freezing and thawing was not related to a change in major membrane protein composition. Human red cell membranes were stable at --80 or --196degreesC in the absence of a cryoprotective agent.", "PMID": 971584}
{"id": "pubmed23n0032_8589", "title": "Survival of frozen-thawed human red cells as a function of cooling and warming velocities.", "content": "Human red cells were equilibrated for 30 min at 20degreesC in buffered saline containing 2 M glycerol and then frozen to --196degreesC at 0.27, 1.7, 59, 180, 480, 600, and 1300degreesC/min and warmed at 0.47, 1, 26, 160, and 550degreesC/min. Cells frozen at 600 and 1300degreesC/min responded in the classical fashion for cells containing intracellular ice; i.e., survivals were low when warming was slow (less than 10%), but increased progressively with increasing warming rate. The sensitivity to slow warming presumably reflects the recrystallization of intracellular ice. Cells frozen at 59 and 180degreesC/min yielded high survivals at all warming rates. This response is also consistent with the findings for other cells cooled just slowly enough to preclude intracellular ice. Cells frozen very slowly at 0.27 and 1.7degreesC/min, however, responded differently; survivals were considerably higher when warming was slow (0.47 or 1degreesC/min) than when it was 26, 160, or 550degreesC/min. This response is analogous to that observed recently by others in mouse embryos and in higher plant tissue-culture cells and to that observed for many years in higher plants. It also confirms previous observations of Meryman in human red cells. It may reflect osmotic shock from rapid dilution but, if so, the basis of the osmotic shock is uncertain.", "contents": "Survival of frozen-thawed human red cells as a function of cooling and warming velocities. Human red cells were equilibrated for 30 min at 20degreesC in buffered saline containing 2 M glycerol and then frozen to --196degreesC at 0.27, 1.7, 59, 180, 480, 600, and 1300degreesC/min and warmed at 0.47, 1, 26, 160, and 550degreesC/min. Cells frozen at 600 and 1300degreesC/min responded in the classical fashion for cells containing intracellular ice; i.e., survivals were low when warming was slow (less than 10%), but increased progressively with increasing warming rate. The sensitivity to slow warming presumably reflects the recrystallization of intracellular ice. Cells frozen at 59 and 180degreesC/min yielded high survivals at all warming rates. This response is also consistent with the findings for other cells cooled just slowly enough to preclude intracellular ice. Cells frozen very slowly at 0.27 and 1.7degreesC/min, however, responded differently; survivals were considerably higher when warming was slow (0.47 or 1degreesC/min) than when it was 26, 160, or 550degreesC/min. This response is analogous to that observed recently by others in mouse embryos and in higher plant tissue-culture cells and to that observed for many years in higher plants. It also confirms previous observations of Meryman in human red cells. It may reflect osmotic shock from rapid dilution but, if so, the basis of the osmotic shock is uncertain.", "PMID": 971585}
{"id": "pubmed23n0032_8590", "title": "A membrane model describing the effect of temperature on the water conductivity of erythrocyte membranes at subzero temperatures.", "content": "Thermodynamic models show that the loss of intracellular water from human erythrocytes during freezing depends heavily upon the water conductivity of the erythrocyte membrane. These calculations, which are based on the simple extrapolation of ambient conductivity data to subzero temperatures, show that more than 95% of cell water is transferable during freezing, whereas experiments show that at least 20% of cell water is retained. A study of the effects of different published values for the membrane water conductivity on cell water retained during freezing shows that this discrepancy may be a consequence of the simple extrapolation procedure. For a homogeneous membrane system, absolute reaction rate theory was used to develop a surface-limited permeation model that includes the resistance to the flow of water not only through the interior region of the membrane but also across possible rate-limiting barriers at the solution-membrane interfaces. The model shows that it is unlikely that a single rate-limiting process dominates water transport in the red cell as it is being cooled from ambient to subzero temperatures. The effective membrane conductivity at subzero temperatures could possible be much lower than a simple extrapolation of existing data would predict. With the aid of this model analytical predictions of intracellular water during freezing are more consistent with experimental observations.", "contents": "A membrane model describing the effect of temperature on the water conductivity of erythrocyte membranes at subzero temperatures. Thermodynamic models show that the loss of intracellular water from human erythrocytes during freezing depends heavily upon the water conductivity of the erythrocyte membrane. These calculations, which are based on the simple extrapolation of ambient conductivity data to subzero temperatures, show that more than 95% of cell water is transferable during freezing, whereas experiments show that at least 20% of cell water is retained. A study of the effects of different published values for the membrane water conductivity on cell water retained during freezing shows that this discrepancy may be a consequence of the simple extrapolation procedure. For a homogeneous membrane system, absolute reaction rate theory was used to develop a surface-limited permeation model that includes the resistance to the flow of water not only through the interior region of the membrane but also across possible rate-limiting barriers at the solution-membrane interfaces. The model shows that it is unlikely that a single rate-limiting process dominates water transport in the red cell as it is being cooled from ambient to subzero temperatures. The effective membrane conductivity at subzero temperatures could possible be much lower than a simple extrapolation of existing data would predict. With the aid of this model analytical predictions of intracellular water during freezing are more consistent with experimental observations.", "PMID": 971586}
{"id": "pubmed23n0032_8591", "title": "The immunological capacity of peripheral lymphocytes in a blast-transformation system using frozen-stored cells.", "content": "Suspensions of isolated peripheral lymphocytes were frozen to --95degreesC using a programmed freezing apparatus and dimethyl sulphoxide as a cryoprotective agent. In comparisons among fresh cells, frozen cells, and cells stored in storage medium, freezing was found to be the best method of storage with retention of almost the same immunocapacity as fresh cells and with the same coefficients of variation for results after stimulation with phytohemagglutinin, pokeweed mitogen, concanavalin A, purified protein derivative, and allogenic cells in mixed lymphocyte cultures as was obtained with fresh cells. Blast transformation was found to be dependent on the number of cells in the cultures, the amount of [14C]thymidine added, and the amount of phytohemagglutinin used by independent of the amounts of pokeweed mitogen and concanavalin A. The maximum responses for normal lymphocytes and lymphocytes from uremic patients after stimulation with phytohemagglutinin occurred simultaneously, but after stimulation with allogenic cells maximum response was obtained earlier for \"uremic\" than for normal lymphocytes. It was concluded that frozen-stored lymphocytes are suitable for in vitro quantitative measurements of the cellular immune response in an immunological sequential study provided that the above mentioned factors are well defined.", "contents": "The immunological capacity of peripheral lymphocytes in a blast-transformation system using frozen-stored cells. Suspensions of isolated peripheral lymphocytes were frozen to --95degreesC using a programmed freezing apparatus and dimethyl sulphoxide as a cryoprotective agent. In comparisons among fresh cells, frozen cells, and cells stored in storage medium, freezing was found to be the best method of storage with retention of almost the same immunocapacity as fresh cells and with the same coefficients of variation for results after stimulation with phytohemagglutinin, pokeweed mitogen, concanavalin A, purified protein derivative, and allogenic cells in mixed lymphocyte cultures as was obtained with fresh cells. Blast transformation was found to be dependent on the number of cells in the cultures, the amount of [14C]thymidine added, and the amount of phytohemagglutinin used by independent of the amounts of pokeweed mitogen and concanavalin A. The maximum responses for normal lymphocytes and lymphocytes from uremic patients after stimulation with phytohemagglutinin occurred simultaneously, but after stimulation with allogenic cells maximum response was obtained earlier for \"uremic\" than for normal lymphocytes. It was concluded that frozen-stored lymphocytes are suitable for in vitro quantitative measurements of the cellular immune response in an immunological sequential study provided that the above mentioned factors are well defined.", "PMID": 971587}
{"id": "pubmed23n0032_8592", "title": "The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen-stored lymphocytes.", "content": "The influence of a series of factors on the frozen storage of lymphocytes was investigated. The cells were frozen using different freezing programmes, using the cryoprotectants dimethyl sulphoxide and glycerol in different concentrations in the freezing medium, and with variations in the period of exposure of cells to cryoprotectants before freezing and after thawing. Cell viability was evaluated by quantitative measurements of the cell-mediated immune response after stimulation with phytohemagglutinin, pokeweed mitogen, concanavalin A, and allogenic cells in mixed lymphocyte cultures. The factors investigated were found to have an important effect on the immune response, so that careful investigation and exact specification of the freezing system are necessary before frozen cells are used in blast-transformation tests. The best freezing programme had a duration of approximately 40 min with a smooth progression through the temperature range where phase transition takes place. The optimum dimethyl sulphoxide concentration in this programme was 8--10%. Dimethyl sulphoxide had no toxic effect on the cells, and no equilibration period was necessary prior to freezing. An equilibration period of 15 min with 10% glycerol was even better than the optimum programme with dimethyl sulphoxide.", "contents": "The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen-stored lymphocytes. The influence of a series of factors on the frozen storage of lymphocytes was investigated. The cells were frozen using different freezing programmes, using the cryoprotectants dimethyl sulphoxide and glycerol in different concentrations in the freezing medium, and with variations in the period of exposure of cells to cryoprotectants before freezing and after thawing. Cell viability was evaluated by quantitative measurements of the cell-mediated immune response after stimulation with phytohemagglutinin, pokeweed mitogen, concanavalin A, and allogenic cells in mixed lymphocyte cultures. The factors investigated were found to have an important effect on the immune response, so that careful investigation and exact specification of the freezing system are necessary before frozen cells are used in blast-transformation tests. The best freezing programme had a duration of approximately 40 min with a smooth progression through the temperature range where phase transition takes place. The optimum dimethyl sulphoxide concentration in this programme was 8--10%. Dimethyl sulphoxide had no toxic effect on the cells, and no equilibration period was necessary prior to freezing. An equilibration period of 15 min with 10% glycerol was even better than the optimum programme with dimethyl sulphoxide.", "PMID": 971588}
{"id": "pubmed23n0032_8593", "title": "Survival of unprotected, mammalian plateau-phase cells following freezing in liquid nitrogen.", "content": "Unprotected, mammalian cells in plateau phase are at least a factor of four times more sensitive to freeze-thaw damage than exponential-phase cells. The former suffer about 15-20% more sublethal damage after one freeze-thaw cycle than the latter and repair this damage more slowly. Exposure of plateau-phase cells to freeze-thaw damage lengthens the time required to traverse the cell cycle in the exposed generation. These cells may more closely represent the state in tissues than exponential-phase populations.", "contents": "Survival of unprotected, mammalian plateau-phase cells following freezing in liquid nitrogen. Unprotected, mammalian cells in plateau phase are at least a factor of four times more sensitive to freeze-thaw damage than exponential-phase cells. The former suffer about 15-20% more sublethal damage after one freeze-thaw cycle than the latter and repair this damage more slowly. Exposure of plateau-phase cells to freeze-thaw damage lengthens the time required to traverse the cell cycle in the exposed generation. These cells may more closely represent the state in tissues than exponential-phase populations.", "PMID": 971589}
{"id": "pubmed23n0032_8594", "title": "Ventilation-perfusion alterations induced by fiberoptic bronchoscopy.", "content": "Pulmonary perfusion (Q) and ventilation (V) scintiphotography was performed in 16 patients undergoing diagnostic fiberoptic bronchoscopic examinations. Regional V/Q did not change in the majority of the patients who developed hypoxemia after bronchoscopic studies. An improvement in V/Q was detectable in the patients with a rise in arterial oxygen pressure (PaO2) after bronchoscopic examination, and this rise was associated in most with the removal of mucous plugs or extensive secretions. The data indicate that the behavior of PaO2 after bronchoscopic study is dependent upon both the extent of lavage and the yield of the procedure in terms of secretions and plugs. The results also indicate that the removal of secretions or plugs can be associated with rapid return of regional V and Q.", "contents": "Ventilation-perfusion alterations induced by fiberoptic bronchoscopy. Pulmonary perfusion (Q) and ventilation (V) scintiphotography was performed in 16 patients undergoing diagnostic fiberoptic bronchoscopic examinations. Regional V/Q did not change in the majority of the patients who developed hypoxemia after bronchoscopic studies. An improvement in V/Q was detectable in the patients with a rise in arterial oxygen pressure (PaO2) after bronchoscopic examination, and this rise was associated in most with the removal of mucous plugs or extensive secretions. The data indicate that the behavior of PaO2 after bronchoscopic study is dependent upon both the extent of lavage and the yield of the procedure in terms of secretions and plugs. The results also indicate that the removal of secretions or plugs can be associated with rapid return of regional V and Q.", "PMID": 971601}
{"id": "pubmed23n0032_8595", "title": "Pressure transducers as a source of bacteremia after open heart surgery. Report of an outbreak and guidelines for prevention.", "content": "During four weeks in 1974, eight (26 percent) of 31 intensive care unit patients who had undergone open-heart surgery developed symptomatic Pseudomonas cepacia bacteremia in the intensive care unit one to three days after the open-heart surgery. An investigation demonstrated that operating room pressure transducers were being contaminated during cleaning with a detergent that contained P cepacia at the rate of 10(4) organisms per milliliter and that the organisms were transmitted to patients after open-heart surgery as a result of one to three days of contact with transducer-monitoring lines used in the operating room and brought to the intensive care unit with the patient. Pressure-transducer contamination, a frequently unappreciated but preventable cause of nosocomial bacteremia, can be minimized by sterilizing transducers between use on different patients by paying strict attention to aseptic technique when setting up, calibrating, and using monitoring systems; and by changing transducers, tubing, and monitoring fluid for each monitored patient at regular intervals.", "contents": "Pressure transducers as a source of bacteremia after open heart surgery. Report of an outbreak and guidelines for prevention. During four weeks in 1974, eight (26 percent) of 31 intensive care unit patients who had undergone open-heart surgery developed symptomatic Pseudomonas cepacia bacteremia in the intensive care unit one to three days after the open-heart surgery. An investigation demonstrated that operating room pressure transducers were being contaminated during cleaning with a detergent that contained P cepacia at the rate of 10(4) organisms per milliliter and that the organisms were transmitted to patients after open-heart surgery as a result of one to three days of contact with transducer-monitoring lines used in the operating room and brought to the intensive care unit with the patient. Pressure-transducer contamination, a frequently unappreciated but preventable cause of nosocomial bacteremia, can be minimized by sterilizing transducers between use on different patients by paying strict attention to aseptic technique when setting up, calibrating, and using monitoring systems; and by changing transducers, tubing, and monitoring fluid for each monitored patient at regular intervals.", "PMID": 971602}
{"id": "pubmed23n0032_8596", "title": "Clinical and roentgenographic manifestations of acute and chronic blastomycosis.", "content": "Roentgenograms and hospital records of 50 patients with blastomycosis proven by cultures were analyzed to determine possible differences between the acute and chronic forms of the illness. Six patients had acute blastomycosis with symptoms occurring three weeks or less prior to hospitalization. The remaining 44 patients had chronic illnesses with symptoms occurring up to six years (mean duration, ten months before hospitalization). The acute illness was characterized by toxic symptoms, chest pain without visible effusions, pneumonic-type consolidations on the chest films, and a relatively benign course. The chronic illness had less severe toxicity, but a high incidence of dissemination, with pleural reactions, hepatosplenomegaly, and cutaneous and osseous involvement. Sputum cultures were positive for Blastomyces dermatitidis in 36 of 46 patients; however, several specimens were frequently required for demonstration of the organism on wet preparations. Pleural fluid and material from skin lesions were also likely sources of the fungus. Skin tests and serologic studies were not helpful in most cases of either the acute or chronic form of the disease.", "contents": "Clinical and roentgenographic manifestations of acute and chronic blastomycosis. Roentgenograms and hospital records of 50 patients with blastomycosis proven by cultures were analyzed to determine possible differences between the acute and chronic forms of the illness. Six patients had acute blastomycosis with symptoms occurring three weeks or less prior to hospitalization. The remaining 44 patients had chronic illnesses with symptoms occurring up to six years (mean duration, ten months before hospitalization). The acute illness was characterized by toxic symptoms, chest pain without visible effusions, pneumonic-type consolidations on the chest films, and a relatively benign course. The chronic illness had less severe toxicity, but a high incidence of dissemination, with pleural reactions, hepatosplenomegaly, and cutaneous and osseous involvement. Sputum cultures were positive for Blastomyces dermatitidis in 36 of 46 patients; however, several specimens were frequently required for demonstration of the organism on wet preparations. Pleural fluid and material from skin lesions were also likely sources of the fungus. Skin tests and serologic studies were not helpful in most cases of either the acute or chronic form of the disease.", "PMID": 971603}
{"id": "pubmed23n0032_8597", "title": "Surgery for congenital heart disease in the adult.", "content": "A total of 205 adults with a variety of congenital heart lesions underwent operation for total correction of their defects. Operative and long-term mortality were 3 and 4 percent, respectively. There has been only one operative death in the past five years (85 patients). While most defects were repaired with good hemodynamic and symptomatic improvement, the three lesions associated with the worst results were cyanotic tetralogy of Fallot, severe pulmonic stenosis complicated by atrial septal defect, and ostium primum atrial septal defect. Myocardial failure due to end-stage myocardial fibrosis was the major cause of operative mortality. Myocardial fibrosis and irreversible pulmonary changes seemed to be the two factors limiting operative correctio", "contents": "Surgery for congenital heart disease in the adult. A total of 205 adults with a variety of congenital heart lesions underwent operation for total correction of their defects. Operative and long-term mortality were 3 and 4 percent, respectively. There has been only one operative death in the past five years (85 patients). While most defects were repaired with good hemodynamic and symptomatic improvement, the three lesions associated with the worst results were cyanotic tetralogy of Fallot, severe pulmonic stenosis complicated by atrial septal defect, and ostium primum atrial septal defect. Myocardial failure due to end-stage myocardial fibrosis was the major cause of operative mortality. Myocardial fibrosis and irreversible pulmonary changes seemed to be the two factors limiting operative correctio", "PMID": 971604}
{"id": "pubmed23n0032_8598", "title": "Transtracheal selective bronchial brushing for pulmonary infiltrates in patients with cancer.", "content": "Patients with cancer frequently develop pneumonitis for which no cause is documented ante mortem. Noninvasive diagnostic techniques, such as sputum induction, are generally inadequate, especially in myelosuppressed patients. To avoid pulmonary contamination with organisms colonizing the oronasopharynx and to obtain uncontaminated speciemens, 38 patients underwent bronchial brushing utilizing a transtracheal approach after sputum induction and transtracheal aspiration failed to establish the etiology. Patients with thrombocytopenia were brushed after platelet transfusion. Eleven patients were not clinically considered to be infected; seven proved to have pulmonary metastases, of which one case was diagnosed by this technique; and four patients in whom no diagnosis was obtained by brushing subsequently proved to have interstitial fibrosis (three cases) or a collapsed lobe (one case). Twenty-seven patients were clinically presumed to be infected. Ultimately, 17 of these 27 patients were proven to have pulmonary infection, and 14 of these 17 were etiologically documented by brushing. In ten of the 27 patients presumed to be infected, no etiology could be established by any method. Seven of these ten patients were receiving broad-spectrum antibiotic therapy at the time. Significant but nonfatal complications, including hemoptysis, pneumothorax, and cervical cellulitis, occurred in seven patients; however, this procedure is a relatively safe and useful method to include in the orderly evaluation of myelosuppressed cancer patients with suspected pulmonary infections.", "contents": "Transtracheal selective bronchial brushing for pulmonary infiltrates in patients with cancer. Patients with cancer frequently develop pneumonitis for which no cause is documented ante mortem. Noninvasive diagnostic techniques, such as sputum induction, are generally inadequate, especially in myelosuppressed patients. To avoid pulmonary contamination with organisms colonizing the oronasopharynx and to obtain uncontaminated speciemens, 38 patients underwent bronchial brushing utilizing a transtracheal approach after sputum induction and transtracheal aspiration failed to establish the etiology. Patients with thrombocytopenia were brushed after platelet transfusion. Eleven patients were not clinically considered to be infected; seven proved to have pulmonary metastases, of which one case was diagnosed by this technique; and four patients in whom no diagnosis was obtained by brushing subsequently proved to have interstitial fibrosis (three cases) or a collapsed lobe (one case). Twenty-seven patients were clinically presumed to be infected. Ultimately, 17 of these 27 patients were proven to have pulmonary infection, and 14 of these 17 were etiologically documented by brushing. In ten of the 27 patients presumed to be infected, no etiology could be established by any method. Seven of these ten patients were receiving broad-spectrum antibiotic therapy at the time. Significant but nonfatal complications, including hemoptysis, pneumothorax, and cervical cellulitis, occurred in seven patients; however, this procedure is a relatively safe and useful method to include in the orderly evaluation of myelosuppressed cancer patients with suspected pulmonary infections.", "PMID": 971605}
{"id": "pubmed23n0032_8599", "title": "Severe airway disease due to inhalation of fumes from cleansing agents.", "content": "A subject had severe airway obstruction and evidence of small-airway disease resulting from inhalation of \"fumes\" liberated during the course of mixing several drain-cleansing agents. The evidence in favor of the airways as the predominant site of damage was severe reduction of maximal expiratory flow in the presence of normal lung-recoil pressure in a nonsmoker who had no other known industrial exposures or predisposing respiratory conditions.", "contents": "Severe airway disease due to inhalation of fumes from cleansing agents. A subject had severe airway obstruction and evidence of small-airway disease resulting from inhalation of \"fumes\" liberated during the course of mixing several drain-cleansing agents. The evidence in favor of the airways as the predominant site of damage was severe reduction of maximal expiratory flow in the presence of normal lung-recoil pressure in a nonsmoker who had no other known industrial exposures or predisposing respiratory conditions.", "PMID": 971606}
{"id": "pubmed23n0032_8600", "title": "Esophageal perforation following use of esophageal airway.", "content": "The esophageal airway has gained increasing acceptance in airway management during cardiopulmonary resuscitation, and its enthusiastic advocates are urging expansion of its use. A fatal case of esophageal perforation following the use of an esophageal airway is presented. Possible contributing factors are discussed. A modified esophageal airway which seems safer and more versatile, effective, and economic is described.", "contents": "Esophageal perforation following use of esophageal airway. The esophageal airway has gained increasing acceptance in airway management during cardiopulmonary resuscitation, and its enthusiastic advocates are urging expansion of its use. A fatal case of esophageal perforation following the use of an esophageal airway is presented. Possible contributing factors are discussed. A modified esophageal airway which seems safer and more versatile, effective, and economic is described.", "PMID": 971607}
{"id": "pubmed23n0032_8601", "title": "Chylopericardium with cardiac tamponade after cardiovascular surgery in two patients.", "content": "Two patients developed postoperative chylopericardium leading to acute cardiac tamponade. Delayed diagnosis and urgent throacotomy did not prevent death in three-month-old patient after the Glenn anastomosis of the superior vena cava to the right pulmonary artery, whereas early recognition of chylopericardium following repair of an interrupted aortic arch in an 11-week-old patient was successfully treated by tube pericardiostomy. The literature is reviewed, and an anatomic predisposition for post-surgical chylopericardium is proposed.", "contents": "Chylopericardium with cardiac tamponade after cardiovascular surgery in two patients. Two patients developed postoperative chylopericardium leading to acute cardiac tamponade. Delayed diagnosis and urgent throacotomy did not prevent death in three-month-old patient after the Glenn anastomosis of the superior vena cava to the right pulmonary artery, whereas early recognition of chylopericardium following repair of an interrupted aortic arch in an 11-week-old patient was successfully treated by tube pericardiostomy. The literature is reviewed, and an anatomic predisposition for post-surgical chylopericardium is proposed.", "PMID": 971608}
{"id": "pubmed23n0032_8602", "title": "Three unusual complications resulting from attempted repair of partial anomalous pulmonary venous drainage.", "content": "The correction of shunts resulting from partial anomalous pulmonary venous drainage has become an accepted surgical procedure. Surgical complications, other than those that were purely postoperative, have been rare. The present report details the case histories of three patients with unusual complications resulting from this type of surgery. Unilateral pulmonary venous obstruction and repeated infections occurred in one patient. In another, obstruction of the superior vena cava resulted. In the third patient, an indaequate operation was performed when the site of partial anomalous pulmonary venous drainage into the coronary sinus was not recognized initially at the time of surgery.", "contents": "Three unusual complications resulting from attempted repair of partial anomalous pulmonary venous drainage. The correction of shunts resulting from partial anomalous pulmonary venous drainage has become an accepted surgical procedure. Surgical complications, other than those that were purely postoperative, have been rare. The present report details the case histories of three patients with unusual complications resulting from this type of surgery. Unilateral pulmonary venous obstruction and repeated infections occurred in one patient. In another, obstruction of the superior vena cava resulted. In the third patient, an indaequate operation was performed when the site of partial anomalous pulmonary venous drainage into the coronary sinus was not recognized initially at the time of surgery.", "PMID": 971609}
{"id": "pubmed23n0032_8603", "title": "Right and light ventricular dimensions as determinants of ventricular septal motion.", "content": "Echocardiographic studies were performed in 86 patients with right ventricular volume overload. In 45 of these 86 patients, 58 echocardiograms were performed at different intervals in the postoperative period. For control purposes, 60 normal subjects and 18 patients with right ventricular pressure overload were also examined. The right ventricular end-diastolic dimension (RVED) and the left ventricular end-diastolic dimension (LVED) were measured on the echocardiograms. The RVED/LVED ratio was significantly elevated in patients with right ventricular volume overload. In all groups, a RVED/LVED ratio above 0.65 was usually associated with an abnormal motion of the septum; and on this basis the subjects with abnormal septal motion could clearly be separated from those with normal motion. After surgery, significant reductions of the RVED/LVED ratio were observed in patients whose septal motion reverted to normal. The RVED/LVED ratio remained significantly higher, usually above 0.65, in patients with persistent abnormal septal motion. These findings suggest that the relative sizes of the right and left ventricular cavities are probably major determinants of ventricular septal motion. Abnormal motion may result from a change in the end-diastolic shape of the septum.", "contents": "Right and light ventricular dimensions as determinants of ventricular septal motion. Echocardiographic studies were performed in 86 patients with right ventricular volume overload. In 45 of these 86 patients, 58 echocardiograms were performed at different intervals in the postoperative period. For control purposes, 60 normal subjects and 18 patients with right ventricular pressure overload were also examined. The right ventricular end-diastolic dimension (RVED) and the left ventricular end-diastolic dimension (LVED) were measured on the echocardiograms. The RVED/LVED ratio was significantly elevated in patients with right ventricular volume overload. In all groups, a RVED/LVED ratio above 0.65 was usually associated with an abnormal motion of the septum; and on this basis the subjects with abnormal septal motion could clearly be separated from those with normal motion. After surgery, significant reductions of the RVED/LVED ratio were observed in patients whose septal motion reverted to normal. The RVED/LVED ratio remained significantly higher, usually above 0.65, in patients with persistent abnormal septal motion. These findings suggest that the relative sizes of the right and left ventricular cavities are probably major determinants of ventricular septal motion. Abnormal motion may result from a change in the end-diastolic shape of the septum.", "PMID": 971610}
{"id": "pubmed23n0032_8604", "title": "Left anterior hemiblock of the 2:1 type in the presence of inferior wall myocardial infarction.", "content": "A case of inferior wall myocardial infarction (IWMI) with left anterior hemiblock (LAH) is presented. The LAH became type 2:1 in the course of the disease. Electrocardiographic signs of LAH in the presence of IWMI are outlined, and the behavior and etiology of the T-wave changes are analyzed.", "contents": "Left anterior hemiblock of the 2:1 type in the presence of inferior wall myocardial infarction. A case of inferior wall myocardial infarction (IWMI) with left anterior hemiblock (LAH) is presented. The LAH became type 2:1 in the course of the disease. Electrocardiographic signs of LAH in the presence of IWMI are outlined, and the behavior and etiology of the T-wave changes are analyzed.", "PMID": 971615}
{"id": "pubmed23n0032_8605", "title": "Intra-His bundle block complicating acute inferior myocardial infarction.", "content": "The unexpected findings of atrioventricular block or delay in the His bundle and proximal branches are described in a 51-year-old man with acute inferior myocardial infarction with narrow QRS complexes. With the information from the His bundle electrogram, the site of atrioventricular block is precisely localized.", "contents": "Intra-His bundle block complicating acute inferior myocardial infarction. The unexpected findings of atrioventricular block or delay in the His bundle and proximal branches are described in a 51-year-old man with acute inferior myocardial infarction with narrow QRS complexes. With the information from the His bundle electrogram, the site of atrioventricular block is precisely localized.", "PMID": 971616}
{"id": "pubmed23n0032_8606", "title": "Cardiac motion in total electrical alternans.", "content": "Gross pendular motion of the heart at half the heart rate was observed during cardiac catheterization and pericardiotomy in a patient with massive pericardial effusion and electrical alternans in the electrocardiogram. These observations support the hypothesis that total electrical alternans in pericardial effusion is a result of alternate change in cardiac position.", "contents": "Cardiac motion in total electrical alternans. Gross pendular motion of the heart at half the heart rate was observed during cardiac catheterization and pericardiotomy in a patient with massive pericardial effusion and electrical alternans in the electrocardiogram. These observations support the hypothesis that total electrical alternans in pericardial effusion is a result of alternate change in cardiac position.", "PMID": 971617}
{"id": "pubmed23n0032_8607", "title": "Clinical experience using a small-gauge needle for arterial puncture.", "content": "The present study was undertaken to ascertain the feasibility of using a 25-gauge needle for arterial punctures. A total of 11,500 arterial punctures were performed over the past four years by this technique without any major complication. Repeated arterial punctures were well tolerated by all patients, and the necessity for indwelling arterial catheters was almost totally eliminated during this period of study.", "contents": "Clinical experience using a small-gauge needle for arterial puncture. The present study was undertaken to ascertain the feasibility of using a 25-gauge needle for arterial punctures. A total of 11,500 arterial punctures were performed over the past four years by this technique without any major complication. Repeated arterial punctures were well tolerated by all patients, and the necessity for indwelling arterial catheters was almost totally eliminated during this period of study.", "PMID": 971621}
{"id": "pubmed23n0032_8608", "title": "Retrieval of embolized central venous catheters by a Dormia ureteral stone dislodger with straight filiform tip.", "content": "Two cases are presented in which a Dormia ureteral stone dislodger with straight filiform tip was used to retrieve broken central venous pressure catheters from the right side of the heart and vena cavae. It should be emphasized that this particular model is more easily passed into the vascular system and more easily manipulated within the vascular system. The delicate filiform tip also contributes to greater safety. It should also be emphasized that entrapment of the proximal tip of a fractured central venous pressure catheter is most ideal prior to extraction.", "contents": "Retrieval of embolized central venous catheters by a Dormia ureteral stone dislodger with straight filiform tip. Two cases are presented in which a Dormia ureteral stone dislodger with straight filiform tip was used to retrieve broken central venous pressure catheters from the right side of the heart and vena cavae. It should be emphasized that this particular model is more easily passed into the vascular system and more easily manipulated within the vascular system. The delicate filiform tip also contributes to greater safety. It should also be emphasized that entrapment of the proximal tip of a fractured central venous pressure catheter is most ideal prior to extraction.", "PMID": 971620}
{"id": "pubmed23n0032_8609", "title": "Anomalous double blood supply to the lung.", "content": "We report an unusual case in which an apparently normal upper lobe of the right lung was supplied by major systemic arterial and pulmonary arterial vessels. The anomalous artery arose from the descending aorta. Following interruption of this vessel, the machinery-like murmur previously present disappeared.", "contents": "Anomalous double blood supply to the lung. We report an unusual case in which an apparently normal upper lobe of the right lung was supplied by major systemic arterial and pulmonary arterial vessels. The anomalous artery arose from the descending aorta. Following interruption of this vessel, the machinery-like murmur previously present disappeared.", "PMID": 971622}
{"id": "pubmed23n0032_8610", "title": "Rate-dependent premature beats in man.", "content": "Ventricular premature beats (VPBs) appeared in a patient after pacemaker insertion for complete heart block secondary to acute myocardial infarction. Contrary to expectations, the frequency of VPBs was directly related to the basic pacemaker rate. The VPBs are either reentrant beats or represent VPBs arising from pacemaker cells with \"slow-response\" characteristics, which have been shown to become more automatic with increasing rates of electrical stimulation. This case documents the phenomenon of rate dependency of VPBs in man and discusses its practical importance.", "contents": "Rate-dependent premature beats in man. Ventricular premature beats (VPBs) appeared in a patient after pacemaker insertion for complete heart block secondary to acute myocardial infarction. Contrary to expectations, the frequency of VPBs was directly related to the basic pacemaker rate. The VPBs are either reentrant beats or represent VPBs arising from pacemaker cells with \"slow-response\" characteristics, which have been shown to become more automatic with increasing rates of electrical stimulation. This case documents the phenomenon of rate dependency of VPBs in man and discusses its practical importance.", "PMID": 971623}
{"id": "pubmed23n0032_8611", "title": "Role of computerized axial tomography in diagnosis and treatment of common neurosurgical problems of infancy and childhood.", "content": "Computerized axial tomography (CAT) is a new, highly accurate technique for studying the skull and intracranial contents. It is rapid enough to be utilized in acute emergencies and safe enough to be utilized for routine serial followups and evaluation of outpatients with equivocal findings. Because CAT can delineate the ventricular system without need for contrast agent, can distinguish clearly between hematoma and edema and can differentiate cystic from solid tumors, it finds wide application in the evaluation of neurological disease.", "contents": "Role of computerized axial tomography in diagnosis and treatment of common neurosurgical problems of infancy and childhood. Computerized axial tomography (CAT) is a new, highly accurate technique for studying the skull and intracranial contents. It is rapid enough to be utilized in acute emergencies and safe enough to be utilized for routine serial followups and evaluation of outpatients with equivocal findings. Because CAT can delineate the ventricular system without need for contrast agent, can distinguish clearly between hematoma and edema and can differentiate cystic from solid tumors, it finds wide application in the evaluation of neurological disease.", "PMID": 971633}
{"id": "pubmed23n0032_8612", "title": "Fetal Chiari's type III malformation.", "content": "The morphological findings in a 4- to 5-month-old female fetus with Chiari's type III malformation are described. This observation strongly supports the view that a defective closure of the roof plate is the primary cause of this malformation, and relates it to the Arnold-Chiari and Dandy-Walker syndromes.", "contents": "Fetal Chiari's type III malformation. The morphological findings in a 4- to 5-month-old female fetus with Chiari's type III malformation are described. This observation strongly supports the view that a defective closure of the roof plate is the primary cause of this malformation, and relates it to the Arnold-Chiari and Dandy-Walker syndromes.", "PMID": 971634}
{"id": "pubmed23n0032_8613", "title": "Ependymomas of childhood. I. Histological survey and clinicopathological correlation.", "content": "We have conducted a correlative histological and clinical study on 34 cases of childhood ependymomas encountered at the Children's Memorial Hospital from 1953 to 1974. The cases were classified into grades A, B and C according to the extent of cytological differentiation and the correlation between the histological grades and the clinical outcome was obtained. It is concluded that the biological behavior of an ependymoma could be predicted to a large extent by the degree of cytological differentiation and by its anatomical location. A large share of the infratentorial ependymomas are well differentiated (grade A) and prognosis in this group is good when complete surgical removal could be accomplished. Most of the supratentorial ependymomas belong to the intermediate grade B and the malignant grade C; prognosis in these two grades is less predictable, approximately two thirds of these patients died within 3 years. The most malignant form is the ependymoblastoma. This is a highly primitive neoplasm histologically similar to medulloblastoma but shows evidence of ependymal differentiation. Most of the ependymoblastomas are located in the supratentorial region and the average length of survival is 20 months.", "contents": "Ependymomas of childhood. I. Histological survey and clinicopathological correlation. We have conducted a correlative histological and clinical study on 34 cases of childhood ependymomas encountered at the Children's Memorial Hospital from 1953 to 1974. The cases were classified into grades A, B and C according to the extent of cytological differentiation and the correlation between the histological grades and the clinical outcome was obtained. It is concluded that the biological behavior of an ependymoma could be predicted to a large extent by the degree of cytological differentiation and by its anatomical location. A large share of the infratentorial ependymomas are well differentiated (grade A) and prognosis in this group is good when complete surgical removal could be accomplished. Most of the supratentorial ependymomas belong to the intermediate grade B and the malignant grade C; prognosis in these two grades is less predictable, approximately two thirds of these patients died within 3 years. The most malignant form is the ependymoblastoma. This is a highly primitive neoplasm histologically similar to medulloblastoma but shows evidence of ependymal differentiation. Most of the ependymoblastomas are located in the supratentorial region and the average length of survival is 20 months.", "PMID": 971635}
{"id": "pubmed23n0032_8614", "title": "Cephalocranial disproportion. A complication of the treatment of hydrocephalus in children.", "content": "Eight children with hydrocephalus showed signs of posterior fossa tonsillar impaction despite arrest of the hydrocephalus and patency of their CSF shunts. Contrast studies performed because of their clinical deterioration demonstrated cerebellar tonsillar herniation, and at posterior fossa decompression, this was confirmed in all cases. Improvement followed decompression. The pathogenesis of a state of cephalocranial disproportion following successful treatment of hydrocephalus resulting in symptomatic tonsillar herniation, is discussed.", "contents": "Cephalocranial disproportion. A complication of the treatment of hydrocephalus in children. Eight children with hydrocephalus showed signs of posterior fossa tonsillar impaction despite arrest of the hydrocephalus and patency of their CSF shunts. Contrast studies performed because of their clinical deterioration demonstrated cerebellar tonsillar herniation, and at posterior fossa decompression, this was confirmed in all cases. Improvement followed decompression. The pathogenesis of a state of cephalocranial disproportion following successful treatment of hydrocephalus resulting in symptomatic tonsillar herniation, is discussed.", "PMID": 971636}
{"id": "pubmed23n0032_8615", "title": "Behavior modification in a residence and school for adolescent boys: a team approach.", "content": "A behavior modification program emphasizing close communication and cooperation between educators and child care workers has proved effective in a school for adolescent boys with learning and behavior problems.", "contents": "Behavior modification in a residence and school for adolescent boys: a team approach. A behavior modification program emphasizing close communication and cooperation between educators and child care workers has proved effective in a school for adolescent boys with learning and behavior problems.", "PMID": 971631}
{"id": "pubmed23n0032_8616", "title": "A truancy program: the child welfare agency and the school.", "content": "A program devised to combine efforts of a child welfare agency and a public school system in combating truancy developed an effective working relationship.", "contents": "A truancy program: the child welfare agency and the school. A program devised to combine efforts of a child welfare agency and a public school system in combating truancy developed an effective working relationship.", "PMID": 971632}
{"id": "pubmed23n0032_8617", "title": "Spinal cord seeding from cerebellar astrocytomas.", "content": "Three juvenile patients with cerebellar astrocytomas which have seeded the spinal subarachnoid space are presented. Histologic verification of the similarity between the posterior fossa tumor and its spinal implant was obtained in two of the three patients. The cerebellar tumors in all cases have been benign (grade I),and the behavior, other than their seeding has also been indolent. Review of pertinent literature discloses no similar experience with cerebellar astrocytomas. Aggressive therapy is advocated for the rare patient with subarachnoid seeding from this benign lesion.", "contents": "Spinal cord seeding from cerebellar astrocytomas. Three juvenile patients with cerebellar astrocytomas which have seeded the spinal subarachnoid space are presented. Histologic verification of the similarity between the posterior fossa tumor and its spinal implant was obtained in two of the three patients. The cerebellar tumors in all cases have been benign (grade I),and the behavior, other than their seeding has also been indolent. Review of pertinent literature discloses no similar experience with cerebellar astrocytomas. Aggressive therapy is advocated for the rare patient with subarachnoid seeding from this benign lesion.", "PMID": 971637}
{"id": "pubmed23n0032_8618", "title": "Headache in classical hemophilia. The risk of diagnostic procedures.", "content": "Headache in a hemophiliac poses a major problem. The fact that at least half of hemophiliacs with proven intracranial hemorrhage present with a headache but no preexisting injury, requires an aggressive approach to the management of these patients. Recent articles point out successful instances of craniotomy in hemophilia, but relatively few hemophiliacs with headaches progress to that stage. We have reviewed the literature and added three cases in an attempt to establish the risk of spinal puncture and cerebral angiography in the evaluation of these patients. The data presented suggests that with replacement therapy and attention to angiographic technique, both procedures may be performed with minimal risk.", "contents": "Headache in classical hemophilia. The risk of diagnostic procedures. Headache in a hemophiliac poses a major problem. The fact that at least half of hemophiliacs with proven intracranial hemorrhage present with a headache but no preexisting injury, requires an aggressive approach to the management of these patients. Recent articles point out successful instances of craniotomy in hemophilia, but relatively few hemophiliacs with headaches progress to that stage. We have reviewed the literature and added three cases in an attempt to establish the risk of spinal puncture and cerebral angiography in the evaluation of these patients. The data presented suggests that with replacement therapy and attention to angiographic technique, both procedures may be performed with minimal risk.", "PMID": 971638}
{"id": "pubmed23n0032_8619", "title": "Subacute radiation myelopathy in a 12-year-old boy.", "content": "Rapid onset over several days of a complete transverse myelopathy occurred 3 1/2 months after completion of mediastinal radiation for Hodgkin's disease in a 12-year-old boy. Postmortem examination revealed low cervical and high thoracic radiation myelopathy. The name \"subacute radiation myelopathy\" is proposed for this type of rapidly developing myelopathy.", "contents": "Subacute radiation myelopathy in a 12-year-old boy. Rapid onset over several days of a complete transverse myelopathy occurred 3 1/2 months after completion of mediastinal radiation for Hodgkin's disease in a 12-year-old boy. Postmortem examination revealed low cervical and high thoracic radiation myelopathy. The name \"subacute radiation myelopathy\" is proposed for this type of rapidly developing myelopathy.", "PMID": 971639}
{"id": "pubmed23n0032_8620", "title": "Recognition of depressed affect in hospitalized psychiatric patients: staff and patient perceptions.", "content": "A comparison of self-report vs. observer rating of depressed mood in a heterogenous inpatient population revealed wide variations in concordance among diagnostic groups. Patients diagnosed as having Affective Psychosis and \"\"Other'' illnesses showed the highest correlation between four self-report scales and an observer rating scale. Patients with a diagnosis of depressive Neurosis showed only modest correlation, while Schizophrenics revealed no significant correlation, on these instruments, suggesting inconsistent communication of affect from Schizophrenic patients to observers. In contrast, when self-report scales were intercorrelated, patients in all four diagnostic categories showed highly significant correlations, indicating that they were consistently reporting their affective state on these instruments. The implications of these findings for future research as well as for practical clinical management are discussed.", "contents": "Recognition of depressed affect in hospitalized psychiatric patients: staff and patient perceptions. A comparison of self-report vs. observer rating of depressed mood in a heterogenous inpatient population revealed wide variations in concordance among diagnostic groups. Patients diagnosed as having Affective Psychosis and \"\"Other'' illnesses showed the highest correlation between four self-report scales and an observer rating scale. Patients with a diagnosis of depressive Neurosis showed only modest correlation, while Schizophrenics revealed no significant correlation, on these instruments, suggesting inconsistent communication of affect from Schizophrenic patients to observers. In contrast, when self-report scales were intercorrelated, patients in all four diagnostic categories showed highly significant correlations, indicating that they were consistently reporting their affective state on these instruments. The implications of these findings for future research as well as for practical clinical management are discussed.", "PMID": 971647}
{"id": "pubmed23n0032_8621", "title": "Group therapy for patients with hysteria (Briquet's disorder).", "content": "It was found that group therapy helped patients with hysteria by (1) satisfying their need to talk (2) lessening their concern about their physical symptoms (3) giving them important knowledge of the illness (4) giving them new ways of handling the illness by themselves (5) giving them the support and the companionship of the other group members (6) reducing their need of medicines (7) reducing psychiatry visits (8) helping them to get along better with their families (9) increasing their self-confidence and (10) improving their mood.", "contents": "Group therapy for patients with hysteria (Briquet's disorder). It was found that group therapy helped patients with hysteria by (1) satisfying their need to talk (2) lessening their concern about their physical symptoms (3) giving them important knowledge of the illness (4) giving them new ways of handling the illness by themselves (5) giving them the support and the companionship of the other group members (6) reducing their need of medicines (7) reducing psychiatry visits (8) helping them to get along better with their families (9) increasing their self-confidence and (10) improving their mood.", "PMID": 971651}
{"id": "pubmed23n0032_8622", "title": "Changes in serum dopamine beta hydroxylase activity related to coma.", "content": "Dopamine Beta Hydroxylase (DBH) activity was determined in 24 deeply comatose patients enrolled in the Collaborative Study of Cerebral Survival. All patients had been without cerebral responsiveness or spontaneous respirations for at least 15 minutes. Patients were examined, EEGs performed and blood for DBH drawn within 24 hours of the cerebral insult. DBH activity was lower in the deeply comatose patients than in a group of 51 age matched normal individuals. It is postulated that destruction or inactivation of the central nervous system (CNS) and particularly of the cerebrum may result in lowered serum DBH activity through decreased activation of sympathetic nerve terminals.", "contents": "Changes in serum dopamine beta hydroxylase activity related to coma. Dopamine Beta Hydroxylase (DBH) activity was determined in 24 deeply comatose patients enrolled in the Collaborative Study of Cerebral Survival. All patients had been without cerebral responsiveness or spontaneous respirations for at least 15 minutes. Patients were examined, EEGs performed and blood for DBH drawn within 24 hours of the cerebral insult. DBH activity was lower in the deeply comatose patients than in a group of 51 age matched normal individuals. It is postulated that destruction or inactivation of the central nervous system (CNS) and particularly of the cerebrum may result in lowered serum DBH activity through decreased activation of sympathetic nerve terminals.", "PMID": 971652}
{"id": "pubmed23n0032_8623", "title": "Regression of bone metastases from renal cell carcinoma achieved by combined therapy.", "content": "A case of renal cell carcinoma with successive multiple bone metastases which survived over a 36-month period is presented. A clinical and radiological regression of bone lesions was obtained by means of combined therapy (hormone therapy, radiotherapy, and immunotherapy), after the original tumor had been removed.", "contents": "Regression of bone metastases from renal cell carcinoma achieved by combined therapy. A case of renal cell carcinoma with successive multiple bone metastases which survived over a 36-month period is presented. A clinical and radiological regression of bone lesions was obtained by means of combined therapy (hormone therapy, radiotherapy, and immunotherapy), after the original tumor had been removed.", "PMID": 971672}
{"id": "pubmed23n0032_8624", "title": "The prognosis of pelviureteric obstruction in childhood: a review of 190 cases.", "content": "190 children with pelviureteric obstruction are reviewed. Their symptoms are analysed and the pre- and post-operative radiographic appearances are assessed. The results are very encouraging with a clinical improvement in the majority of patients, free ureteric drainage in almost all and an improvement in the calyceal pattern in 65.9%.", "contents": "The prognosis of pelviureteric obstruction in childhood: a review of 190 cases. 190 children with pelviureteric obstruction are reviewed. Their symptoms are analysed and the pre- and post-operative radiographic appearances are assessed. The results are very encouraging with a clinical improvement in the majority of patients, free ureteric drainage in almost all and an improvement in the calyceal pattern in 65.9%.", "PMID": 971674}
{"id": "pubmed23n0032_8625", "title": "The single ectopic ureter.", "content": "32 cases of single ectopic ureter have been reviewed. The more remote the ectopic orifice opens from its normal position the more severe were the associated renal anomalies. In the great majority of cases, the corresponding kidney was dysplastic and radiographically functionless. An embryological explanation is proposed to account for the association of single ectopic ureter with a dysplastic kidney. Clinical symptomatology was dribbling incontinence in most female cases and various urogenital complaints in male. An accurate preoperative diagnosis is usually reached with a pyelogram and micturating cystogram. In some female cases a vaginogram or direct puncture and opacification of a vaginal cystic mass may be contributive. In the male, a radiological non-functioning kidney on one side of the urogram associated with a cystic mass palpated above the prostate is pathognomonic of an ectopic ureter opening in the seminal tract. Deferentography may confirm this diagnosis. Treatment of the condition is surgical. In the great majority of cases nephroureterectomy is required. In the male, the abnormal seminal tract involved in this complex malformation should also be removed.", "contents": "The single ectopic ureter. 32 cases of single ectopic ureter have been reviewed. The more remote the ectopic orifice opens from its normal position the more severe were the associated renal anomalies. In the great majority of cases, the corresponding kidney was dysplastic and radiographically functionless. An embryological explanation is proposed to account for the association of single ectopic ureter with a dysplastic kidney. Clinical symptomatology was dribbling incontinence in most female cases and various urogenital complaints in male. An accurate preoperative diagnosis is usually reached with a pyelogram and micturating cystogram. In some female cases a vaginogram or direct puncture and opacification of a vaginal cystic mass may be contributive. In the male, a radiological non-functioning kidney on one side of the urogram associated with a cystic mass palpated above the prostate is pathognomonic of an ectopic ureter opening in the seminal tract. Deferentography may confirm this diagnosis. Treatment of the condition is surgical. In the great majority of cases nephroureterectomy is required. In the male, the abnormal seminal tract involved in this complex malformation should also be removed.", "PMID": 971675}
{"id": "pubmed23n0032_8626", "title": "Partial nephrectomy for renal tumour: 21 cases.", "content": "Partial nephrectomy has been performed in 21 cases of kidney tumours. It can be used for well-limited parenchymatous tumours and obligatory in patients with a solitary kidney, but is not advisable for tumours originating in the urothelium. These results are compared with the parallel experience with radical surgery and followed during 46 years in the same centre, they give a greater value to this study and encourage the reasoned practice of partial nephrectomy for tumours especially in the solitary kidney.", "contents": "Partial nephrectomy for renal tumour: 21 cases. Partial nephrectomy has been performed in 21 cases of kidney tumours. It can be used for well-limited parenchymatous tumours and obligatory in patients with a solitary kidney, but is not advisable for tumours originating in the urothelium. These results are compared with the parallel experience with radical surgery and followed during 46 years in the same centre, they give a greater value to this study and encourage the reasoned practice of partial nephrectomy for tumours especially in the solitary kidney.", "PMID": 971676}
{"id": "pubmed23n0032_8627", "title": "Treatment of interstitial cystitis with immunosuppression and chloroquine derivatives.", "content": "Azathioprine, an immunosuppressive agent, was given to 38 patients presenting with intestinal cystitis. Pain disappeared completely in 22 patients and pollakiuria did the same in 20 patients, including two very severe cases with very contracted bladder. Chloroquine or oxychloroquine, usually combined with salicylate, was given to 22 patients. Pain disappeared in 11 patients and pollakiuria in 4 patients. No effect was noted in 10 patients in the azathioprine group and in 8 patients in the chloroquine group. The remaining patients had partial alleviation. The results are analyzed separately for various grades of severity. Spontaneous cure can be expected only in 11% of cases of interstitial cystitis calculated from a large series. Immunosuppression or chloroquine derivates are indicated for patients who do not respond to other treatments.", "contents": "Treatment of interstitial cystitis with immunosuppression and chloroquine derivatives. Azathioprine, an immunosuppressive agent, was given to 38 patients presenting with intestinal cystitis. Pain disappeared completely in 22 patients and pollakiuria did the same in 20 patients, including two very severe cases with very contracted bladder. Chloroquine or oxychloroquine, usually combined with salicylate, was given to 22 patients. Pain disappeared in 11 patients and pollakiuria in 4 patients. No effect was noted in 10 patients in the azathioprine group and in 8 patients in the chloroquine group. The remaining patients had partial alleviation. The results are analyzed separately for various grades of severity. Spontaneous cure can be expected only in 11% of cases of interstitial cystitis calculated from a large series. Immunosuppression or chloroquine derivates are indicated for patients who do not respond to other treatments.", "PMID": 971677}
{"id": "pubmed23n0032_8628", "title": "Endocrine effects of oestrogen treatment in patients with prostatic cancer.", "content": "In 36 men with prostatic cancer, the following findings were obtained: intravenous administration of 12.0 g diethylstilboestrol diphosphate (DSDP) induced a relatively slight decrease of the LH plasma level from 22.7 +/- 11.8 to 7.7 +/- 3.6 mIU/ml (34%), whereas the total testosterone plasma level decreased from 435.3 +/- 187.8 to 29.9 +/- 16.4 ng/100 ml (6.7%) suggesting a direct inhibitory effect of the oestrogen on testicular testosterone secretion. The apparently free, biologically active testosterone plasma level even decreased from 6.2 +/- 3.7 to 0.21 +/- 0.16 ng/100 ml (3.4%), due to the oestrogen-induced increase of the concentration of testosterone-binding beta-globulin (from 9.6 +/- 4.4 to 20.6 +/- 10.7-10(-8) M). 3--7 days after additional orchidectomy plus subcutaneous implantation of 100 mg dienoestrol diacetate a further decrease of the apparently free testosterone plasma level from 0.21 +/- 0.16 to 0.14 +/- 0.07 ng/100 ml was found. In contrast, 6 weeks after orchidectomy without oestrogen implantation a significant increase of th- apparently free testosterone plasma level -rom 0.21 %/- 0.16 to 0.34 +/- 0.15 ng/100 ml was observed (p less than 0.01). In view of these findings the biologically active free testosterone plasma level appears to be even more suppressed by intravenous administration of high DSDP than by orchidectomy. The most effective suppression of apparently free testosterone was achieved, however, by oestrogen treatment combined with orchidectomy.", "contents": "Endocrine effects of oestrogen treatment in patients with prostatic cancer. In 36 men with prostatic cancer, the following findings were obtained: intravenous administration of 12.0 g diethylstilboestrol diphosphate (DSDP) induced a relatively slight decrease of the LH plasma level from 22.7 +/- 11.8 to 7.7 +/- 3.6 mIU/ml (34%), whereas the total testosterone plasma level decreased from 435.3 +/- 187.8 to 29.9 +/- 16.4 ng/100 ml (6.7%) suggesting a direct inhibitory effect of the oestrogen on testicular testosterone secretion. The apparently free, biologically active testosterone plasma level even decreased from 6.2 +/- 3.7 to 0.21 +/- 0.16 ng/100 ml (3.4%), due to the oestrogen-induced increase of the concentration of testosterone-binding beta-globulin (from 9.6 +/- 4.4 to 20.6 +/- 10.7-10(-8) M). 3--7 days after additional orchidectomy plus subcutaneous implantation of 100 mg dienoestrol diacetate a further decrease of the apparently free testosterone plasma level from 0.21 +/- 0.16 to 0.14 +/- 0.07 ng/100 ml was found. In contrast, 6 weeks after orchidectomy without oestrogen implantation a significant increase of th- apparently free testosterone plasma level -rom 0.21 %/- 0.16 to 0.34 +/- 0.15 ng/100 ml was observed (p less than 0.01). In view of these findings the biologically active free testosterone plasma level appears to be even more suppressed by intravenous administration of high DSDP than by orchidectomy. The most effective suppression of apparently free testosterone was achieved, however, by oestrogen treatment combined with orchidectomy.", "PMID": 971678}
{"id": "pubmed23n0032_8629", "title": "Crystaloptical and spectroscopical findings with calcium oxalate crystals in the urine sediment: a contribution to the genesis of oxalate stones.", "content": "Transmitted light microscope and scanning electron microscope investigations reveal various shapes of urine calcium oxalate crystals. In addition to tetragonal bipyramids, weddellite forms further crystal shapes that have been heretofore interpreted exclusively as whewellite crystals. Weddellite is stabilized by urine foreign ions. In vivo formation of whewellite crystals occurs with massive crystallization only.", "contents": "Crystaloptical and spectroscopical findings with calcium oxalate crystals in the urine sediment: a contribution to the genesis of oxalate stones. Transmitted light microscope and scanning electron microscope investigations reveal various shapes of urine calcium oxalate crystals. In addition to tetragonal bipyramids, weddellite forms further crystal shapes that have been heretofore interpreted exclusively as whewellite crystals. Weddellite is stabilized by urine foreign ions. In vivo formation of whewellite crystals occurs with massive crystallization only.", "PMID": 971679}
{"id": "pubmed23n0032_8630", "title": "Chemical sympathectomy with phenol for chronic prostatic pain.", "content": "A case of chronic prostatitis where conventional chemotherapy and repeated transurethral resections failed to relieve pain is described. A cutaneous uretero-entero anastomosis was performed for troublesome pain and incontinence and only a permanent sympathetic block with phenol at the level of L4 achieved complete pain relief.", "contents": "Chemical sympathectomy with phenol for chronic prostatic pain. A case of chronic prostatitis where conventional chemotherapy and repeated transurethral resections failed to relieve pain is described. A cutaneous uretero-entero anastomosis was performed for troublesome pain and incontinence and only a permanent sympathetic block with phenol at the level of L4 achieved complete pain relief.", "PMID": 971680}
{"id": "pubmed23n0032_8631", "title": "Nephroureterectomy in urinary tuberculosis.", "content": "130 total nephroureterectomies were performed for advanced renal tuberculosis with multiple ureteric stenoses. The important part played by ureteric lesions in maintaining vesical foci after simple nephrectomy is emphasised. The operation performed through the lumbar and intraperitoneal route does not carry any risk.", "contents": "Nephroureterectomy in urinary tuberculosis. 130 total nephroureterectomies were performed for advanced renal tuberculosis with multiple ureteric stenoses. The important part played by ureteric lesions in maintaining vesical foci after simple nephrectomy is emphasised. The operation performed through the lumbar and intraperitoneal route does not carry any risk.", "PMID": 971681}
{"id": "pubmed23n0032_8632", "title": "Evaluation of histological malignant stages of epithelial bladder tumours: a simplified classification.", "content": "A simplified practical histological classification of four stages of bladder epithelial tumours is proposed in accordance with the terminology adopted by WHO. The structural characters of neoplastic elements are considered with respect to their relations with the basal membrane delimiting the epithelia. This criterion gives valid information regarding real malignancy of the tumour and may be applied to biopsies obtained endoscopically. This is very important not only with regard to prognosis but also to therapeutic indications.", "contents": "Evaluation of histological malignant stages of epithelial bladder tumours: a simplified classification. A simplified practical histological classification of four stages of bladder epithelial tumours is proposed in accordance with the terminology adopted by WHO. The structural characters of neoplastic elements are considered with respect to their relations with the basal membrane delimiting the epithelia. This criterion gives valid information regarding real malignancy of the tumour and may be applied to biopsies obtained endoscopically. This is very important not only with regard to prognosis but also to therapeutic indications.", "PMID": 971682}
{"id": "pubmed23n0032_8633", "title": "Normal urographic findings in Nigerians.", "content": "A review of excretory urographic studies in Nigerians at the Lagos University Teaching Hospital shows that the average renal lengths in adult patients is 12.2 cm. Male patients had slightly longer kidneys than females while the left kidney was longer and higher than the right in most cases and in both sexes. There was symmetry in the pelvicaliceal architecture of both kidneys of the same patient in 85% of cases while disparity in renal length for the same patient was less than 1.5 cm in 89% of cases. Pelviureteric duplication was seen in 4.4% of patients with a male to female ratio of 2:1. The results obtained for the Nigerian patients are not significantly different from those obtained by other authors on caucasians.", "contents": "Normal urographic findings in Nigerians. A review of excretory urographic studies in Nigerians at the Lagos University Teaching Hospital shows that the average renal lengths in adult patients is 12.2 cm. Male patients had slightly longer kidneys than females while the left kidney was longer and higher than the right in most cases and in both sexes. There was symmetry in the pelvicaliceal architecture of both kidneys of the same patient in 85% of cases while disparity in renal length for the same patient was less than 1.5 cm in 89% of cases. Pelviureteric duplication was seen in 4.4% of patients with a male to female ratio of 2:1. The results obtained for the Nigerian patients are not significantly different from those obtained by other authors on caucasians.", "PMID": 971683}
{"id": "pubmed23n0032_8634", "title": "Ureteric obstruction associated with uterine prolapse.", "content": "14 cases of ureteric obstruction in patients with uterine prolapse are presented. The clinical, radiological, endoscopic and surgical observations are considered and discussed. Ureteric dilatation develops in two successive stages each of which has a different mechanism. In the first stage, stretching of the terminal ureter leads to a fusiform and gradual dilatation. In the second more advanced stage, caudal and posterior displacement of the trigone and bladder base leads to a rotation of the intramural ureter forming a sharp angle with the juxtavesical ureter, causing further ureteric obstruction. These observations emphasize the importance of urographical evaluation in patients with uterine prolapse.", "contents": "Ureteric obstruction associated with uterine prolapse. 14 cases of ureteric obstruction in patients with uterine prolapse are presented. The clinical, radiological, endoscopic and surgical observations are considered and discussed. Ureteric dilatation develops in two successive stages each of which has a different mechanism. In the first stage, stretching of the terminal ureter leads to a fusiform and gradual dilatation. In the second more advanced stage, caudal and posterior displacement of the trigone and bladder base leads to a rotation of the intramural ureter forming a sharp angle with the juxtavesical ureter, causing further ureteric obstruction. These observations emphasize the importance of urographical evaluation in patients with uterine prolapse.", "PMID": 971684}
{"id": "pubmed23n0032_8635", "title": "The physiology and pathophysiology of the ureter.", "content": "A review is given of the theories concerning the transport of urine from the calyces to the bladder. The application of hydrodynamic laws on the propulsion of urine, the visco-elastic characteristics of the ureter wall, the myogenic versus the neurogenic origin of spontaneous activity of the ureter and the presence of pacemaker areas, are discussed. The changes of peristaltic activity and intraluminal pressure during obstruction, infection and reflux are summarized.", "contents": "The physiology and pathophysiology of the ureter. A review is given of the theories concerning the transport of urine from the calyces to the bladder. The application of hydrodynamic laws on the propulsion of urine, the visco-elastic characteristics of the ureter wall, the myogenic versus the neurogenic origin of spontaneous activity of the ureter and the presence of pacemaker areas, are discussed. The changes of peristaltic activity and intraluminal pressure during obstruction, infection and reflux are summarized.", "PMID": 971685}
{"id": "pubmed23n0032_8636", "title": "Metabolism of digitoxin in man and its modification by spironolactone.", "content": "The effect of spironolactone on the metabolism of intravenously administered 3H-digitoxin (80 muCi) was investigated in eight patients. In three of them the labelled glycoside was given on a second occasion after spironolactone treatment had been discontinued for at least 65 days. Of total urinary radioactivity 79% was unaltered drug and 12% consisted of water soluble compounds. No digitoxigenin or digoxigenin and only trace amounts (less than 2 %) of digoxin and the bis- and monoglycosides of digoxigenin were found. After spironolactone total urinary radioactivity was unchanged but the fraction eliminated as unchanged digitoxin fell from 79 to 66 % and the water soluble compounds increased from 12 to 26 % (p less than 0.05). In addition spironolactone caused a 20 ( reduction in the half-life of serum radioactivity (p less than 0.01) and a  16 % reduction in the volume of distribution (p less than 0.05). Induction of hepatic enzymes by spironolactone is proposed to explain the alteration in the metabolism of digitoxin in man. Both the altered metabolic pattern and the reduction in the volume of distribution appear to contribute to the reduction in half-life.", "contents": "Metabolism of digitoxin in man and its modification by spironolactone. The effect of spironolactone on the metabolism of intravenously administered 3H-digitoxin (80 muCi) was investigated in eight patients. In three of them the labelled glycoside was given on a second occasion after spironolactone treatment had been discontinued for at least 65 days. Of total urinary radioactivity 79% was unaltered drug and 12% consisted of water soluble compounds. No digitoxigenin or digoxigenin and only trace amounts (less than 2 %) of digoxin and the bis- and monoglycosides of digoxigenin were found. After spironolactone total urinary radioactivity was unchanged but the fraction eliminated as unchanged digitoxin fell from 79 to 66 % and the water soluble compounds increased from 12 to 26 % (p less than 0.05). In addition spironolactone caused a 20 ( reduction in the half-life of serum radioactivity (p less than 0.01) and a  16 % reduction in the volume of distribution (p less than 0.05). Induction of hepatic enzymes by spironolactone is proposed to explain the alteration in the metabolism of digitoxin in man. Both the altered metabolic pattern and the reduction in the volume of distribution appear to contribute to the reduction in half-life.", "PMID": 971699}
{"id": "pubmed23n0032_8637", "title": "Mechanism of the interaction of propranolol and a potent vasodilator antihypertensive agent - minoxidil.", "content": "A study, using non-invasive techniques, was carried out in ten patients with essential hypertension to examine the mechanism of the hypotensive effect of propranolol when used in combination with a potent vasodilator antihypertensive - minoxidil. The hypotensive effect of minoxidil, a mean (+/- SEM) decrease of 42.4 +/- 4.3 mm Hg, was accompanied by a marked increase in heart rate, cardiac index and plasma renin activity and a significant decrease in total peripheral resistance, limb vascular resistance and pre-ejection period. Addition of propranolol further reduced mean arterial pressure by an average of 12.9 +/- 2.0 mm Hg. Propranolol returned cardiac index to control values and total peripheral resistance index rose but not to control levels. Plasma renin activity was significantly reduced by propranolol. By multiple regression analysis no correlation was found between propranolol-induced decrease in mean arterial pressure and changes in cardiac index, total peripheral resistance index or plasma renin activity. Quantitatively, the reduction in cardiac index observed probably accounted for the hypotensive effect of propranolol. The role of plasma renin activity reduction in the hypotensive effect of propranolol in this situation remains to be clarified. The findings in the present study were consonant with the known actions of vasodilator antihypertensive agents and propranolol and indicate the applicability of non-invasive methodology to the investigation of cardiovascular drugs in man.", "contents": "Mechanism of the interaction of propranolol and a potent vasodilator antihypertensive agent - minoxidil. A study, using non-invasive techniques, was carried out in ten patients with essential hypertension to examine the mechanism of the hypotensive effect of propranolol when used in combination with a potent vasodilator antihypertensive - minoxidil. The hypotensive effect of minoxidil, a mean (+/- SEM) decrease of 42.4 +/- 4.3 mm Hg, was accompanied by a marked increase in heart rate, cardiac index and plasma renin activity and a significant decrease in total peripheral resistance, limb vascular resistance and pre-ejection period. Addition of propranolol further reduced mean arterial pressure by an average of 12.9 +/- 2.0 mm Hg. Propranolol returned cardiac index to control values and total peripheral resistance index rose but not to control levels. Plasma renin activity was significantly reduced by propranolol. By multiple regression analysis no correlation was found between propranolol-induced decrease in mean arterial pressure and changes in cardiac index, total peripheral resistance index or plasma renin activity. Quantitatively, the reduction in cardiac index observed probably accounted for the hypotensive effect of propranolol. The role of plasma renin activity reduction in the hypotensive effect of propranolol in this situation remains to be clarified. The findings in the present study were consonant with the known actions of vasodilator antihypertensive agents and propranolol and indicate the applicability of non-invasive methodology to the investigation of cardiovascular drugs in man.", "PMID": 971700}
{"id": "pubmed23n0032_8638", "title": "Observations on the efficacy and pharmacokinetics of sotalol after oral administration.", "content": "The effects of sotalol after oral administration were measured on the tachycardia induced by strenuous exercise in normal subjects. Plasma sotalol levels were also determined. The oral administration of sotalol (50, 100, 200 and 400 mg) to 6 subjects produced a progressive reduction in the tachycardia induced by severe exercise. This was similar to the effects of 25, 50, 100, 200, 400 and 800 mg given to different subjects. Each increase in sotalol dose produced a successively greater reduction in exercise tachycardia. This did not appear to be maximum even with 800 mg. Oral sotalol was rapidly absorbed and produced peak blood levels in 2 - 3 hours. The plasma levels of sotalol measured 2 hours after the oral administration of 25 to 800 mg showed never more than a six-fold variation between different subject. The half-life of sotalol in plasma was 12.7 +/- SE 1.6 hours. There was a significant correlation between the logarithm of the plasma sotalol concentration and the percentage reduction of exercise heart rate. It is concluded that the oral administration of sotalol either once or twice daily (depending on dose level) will provide satisfactory 24-hour blockade of beta-adrenoceptors.", "contents": "Observations on the efficacy and pharmacokinetics of sotalol after oral administration. The effects of sotalol after oral administration were measured on the tachycardia induced by strenuous exercise in normal subjects. Plasma sotalol levels were also determined. The oral administration of sotalol (50, 100, 200 and 400 mg) to 6 subjects produced a progressive reduction in the tachycardia induced by severe exercise. This was similar to the effects of 25, 50, 100, 200, 400 and 800 mg given to different subjects. Each increase in sotalol dose produced a successively greater reduction in exercise tachycardia. This did not appear to be maximum even with 800 mg. Oral sotalol was rapidly absorbed and produced peak blood levels in 2 - 3 hours. The plasma levels of sotalol measured 2 hours after the oral administration of 25 to 800 mg showed never more than a six-fold variation between different subject. The half-life of sotalol in plasma was 12.7 +/- SE 1.6 hours. There was a significant correlation between the logarithm of the plasma sotalol concentration and the percentage reduction of exercise heart rate. It is concluded that the oral administration of sotalol either once or twice daily (depending on dose level) will provide satisfactory 24-hour blockade of beta-adrenoceptors.", "PMID": 971701}
{"id": "pubmed23n0032_8639", "title": "Bioavailability and pharmacokinetics of beta-methyldigoxin after multiple oral and intravenous doses.", "content": "To obtain true half lives, glycoside elimination from six healthy subjects was studied for 14 days after multiple intravenous doses or oral administration of a daily maintenance dose of beta-methyldigoxin 0.3 mg. After oral or intravenous administration of beta-methyldigoxin ceased, the plasma concentrations declined from the 14th to the 16th days with a half life of 1.7 days. From the 16th to the 20th day a change from a shorter to a longer half life of 2.8 and 2.9 days was observed. Similar half lives were found in urine: after the last dose the initial slope from the 14th to the 16th day had a half life of 1.8 days, and the terminal slope had one of 3.2 days. The results indicate release of the glycoside from slowly equilibrating tissues.", "contents": "Bioavailability and pharmacokinetics of beta-methyldigoxin after multiple oral and intravenous doses. To obtain true half lives, glycoside elimination from six healthy subjects was studied for 14 days after multiple intravenous doses or oral administration of a daily maintenance dose of beta-methyldigoxin 0.3 mg. After oral or intravenous administration of beta-methyldigoxin ceased, the plasma concentrations declined from the 14th to the 16th days with a half life of 1.7 days. From the 16th to the 20th day a change from a shorter to a longer half life of 2.8 and 2.9 days was observed. Similar half lives were found in urine: after the last dose the initial slope from the 14th to the 16th day had a half life of 1.8 days, and the terminal slope had one of 3.2 days. The results indicate release of the glycoside from slowly equilibrating tissues.", "PMID": 971702}
{"id": "pubmed23n0032_8640", "title": "Comparative clinical pharmacology of intravenous cefoxitin and cephalothin.", "content": "Intravenous doses of 0.5, 1, and 2 g cephalothin and cefoxitin, a semi-synthetic cephamycin antibiotic highly resistant to bacterial cephalosporinase, were infused over a period of 3 minutes into 18 normal adult males by a randomized, crossover design. Serum and urine data on cefoxitin best fit a two-compartment open model. Serum concentrations following cefoxitin were higher and more prolonged and urine recoveries higher than those following equal doses of cephalothin. The terminal serum half-life of cefoxitin was longer at all dose levels. Renal clearance of cephalothin-like activity exceeded that of cefoxitin, which may possess dose-dependent kinetics. Whereas cephalothin has been reported to metabolize by greater than 35% to the less active desacetyl form, cefoxitin was metabolized by 0.1 to 6% to the descarbamyl form in individual subjects.", "contents": "Comparative clinical pharmacology of intravenous cefoxitin and cephalothin. Intravenous doses of 0.5, 1, and 2 g cephalothin and cefoxitin, a semi-synthetic cephamycin antibiotic highly resistant to bacterial cephalosporinase, were infused over a period of 3 minutes into 18 normal adult males by a randomized, crossover design. Serum and urine data on cefoxitin best fit a two-compartment open model. Serum concentrations following cefoxitin were higher and more prolonged and urine recoveries higher than those following equal doses of cephalothin. The terminal serum half-life of cefoxitin was longer at all dose levels. Renal clearance of cephalothin-like activity exceeded that of cefoxitin, which may possess dose-dependent kinetics. Whereas cephalothin has been reported to metabolize by greater than 35% to the less active desacetyl form, cefoxitin was metabolized by 0.1 to 6% to the descarbamyl form in individual subjects.", "PMID": 971703}
{"id": "pubmed23n0032_8641", "title": "Plasma levels of carbamazepine and carbamazepine-10,11-epoxide during treatment of epilepsy.", "content": "Carbamazepine and its epoxide in plasma were measured by liquid chromatography in 25 patients treated with a mean dose of carbamazepine of 12.5 +/- 3.3 mg/kg body weight. The mean concentrations of parent drug and metabolite were 5.4 +/- 2.5 mug/ml and 1.10 +/- 0.42 mug/ml, respectively. A singificant correlation was found between the plasma concentrations of the two compounds (r = 0.64; p less than 0.001), but marked interindividual variation existed in the ratio of carbamazepine to carbamazepine to epoxide. Based on simultaneous measurements in plasma and cerebrospinal fluid, the unbound fraction of carbamazepine in plasma was of the order of 20% as compared to 45% for the epoxide. Thirteen ambulant patients suffering from partial epilepsy with complex symptomatology, who were already being treated with phenytoin in optimal doses (plasma level 14-20 mug/ml) were also given carbamazepine. At plasma levels of the latter of about 5 mug/ml there was no further reduction in the frequency of partial or generalized epileptic seizures. In five patients the dose was increased to produce plasma concentrations of 7 - 8 mug/ml. There was still no improvement and side-effects were seen in three patients.", "contents": "Plasma levels of carbamazepine and carbamazepine-10,11-epoxide during treatment of epilepsy. Carbamazepine and its epoxide in plasma were measured by liquid chromatography in 25 patients treated with a mean dose of carbamazepine of 12.5 +/- 3.3 mg/kg body weight. The mean concentrations of parent drug and metabolite were 5.4 +/- 2.5 mug/ml and 1.10 +/- 0.42 mug/ml, respectively. A singificant correlation was found between the plasma concentrations of the two compounds (r = 0.64; p less than 0.001), but marked interindividual variation existed in the ratio of carbamazepine to carbamazepine to epoxide. Based on simultaneous measurements in plasma and cerebrospinal fluid, the unbound fraction of carbamazepine in plasma was of the order of 20% as compared to 45% for the epoxide. Thirteen ambulant patients suffering from partial epilepsy with complex symptomatology, who were already being treated with phenytoin in optimal doses (plasma level 14-20 mug/ml) were also given carbamazepine. At plasma levels of the latter of about 5 mug/ml there was no further reduction in the frequency of partial or generalized epileptic seizures. In five patients the dose was increased to produce plasma concentrations of 7 - 8 mug/ml. There was still no improvement and side-effects were seen in three patients.", "PMID": 971704}
{"id": "pubmed23n0032_8642", "title": "Some aspects of the intestinal absorption of zinc in man.", "content": "Serum zinc concentrations in peripheral venous blood were determined in 8 healthy volunteers at various times after oral administration of 50 mg Zn++. The same dose was given to 6 patients surgically treated for obesity by jejuno-ileostomy. In the healthy volunteers the mean serum zinc concentration before dosing was 0.89 mug/ml and a mean peak concentration of 2.39 mug/ml was found after 3 h. In the patients the starting level was lower, 0.67 mug/ml, and a mean peak concentration of 1.31 mug/ml was found 90 min after treatment. In the patients the areas under the serum concentration-time curve was approximately 1/3 of that in the healthy subjects. Zn++ 50 mg was also given to 3 patients undergoing transumbilical catheterization of the portal vein for diagnostic purposes and serum zinc concentrations were measured in portal and peripheral venous blood sampled simultaneously. No significant differences were found between the concentration of zinc in portal and peripheral venous blood during absorption, which suggests slow passage of zinc across the intestinal wall.", "contents": "Some aspects of the intestinal absorption of zinc in man. Serum zinc concentrations in peripheral venous blood were determined in 8 healthy volunteers at various times after oral administration of 50 mg Zn++. The same dose was given to 6 patients surgically treated for obesity by jejuno-ileostomy. In the healthy volunteers the mean serum zinc concentration before dosing was 0.89 mug/ml and a mean peak concentration of 2.39 mug/ml was found after 3 h. In the patients the starting level was lower, 0.67 mug/ml, and a mean peak concentration of 1.31 mug/ml was found 90 min after treatment. In the patients the areas under the serum concentration-time curve was approximately 1/3 of that in the healthy subjects. Zn++ 50 mg was also given to 3 patients undergoing transumbilical catheterization of the portal vein for diagnostic purposes and serum zinc concentrations were measured in portal and peripheral venous blood sampled simultaneously. No significant differences were found between the concentration of zinc in portal and peripheral venous blood during absorption, which suggests slow passage of zinc across the intestinal wall.", "PMID": 971705}
{"id": "pubmed23n0032_8643", "title": "Decreased drug binding in serum from patients with chronic hepatic disease.", "content": "The binding of 3 drugs to serum proteins of patients with chronic hepatic disease has been studied by an ultrafilitration technique, and compared to that of normal subjects. The binding of phenylbutazone was reduced in all patients, salicylate in patients with inactive liver disease and sulphadiazine in patients whose disease was active. Analysis of binding data showed a real reduction in the capacity of albumin to bind the drugs in the majority of patients. Addition of bilirubin to normal plasma caused a reduction in sulphadiazine binding, but had no effect on the binding of salicylate or phenylbutazone. The possible causes of this reduction in binding are discussed.", "contents": "Decreased drug binding in serum from patients with chronic hepatic disease. The binding of 3 drugs to serum proteins of patients with chronic hepatic disease has been studied by an ultrafilitration technique, and compared to that of normal subjects. The binding of phenylbutazone was reduced in all patients, salicylate in patients with inactive liver disease and sulphadiazine in patients whose disease was active. Analysis of binding data showed a real reduction in the capacity of albumin to bind the drugs in the majority of patients. Addition of bilirubin to normal plasma caused a reduction in sulphadiazine binding, but had no effect on the binding of salicylate or phenylbutazone. The possible causes of this reduction in binding are discussed.", "PMID": 971706}
{"id": "pubmed23n0032_8644", "title": "Comparison of the acetylation of procainamide and sulfadimidine in man.", "content": "The acetylation of procainamide and sulfadimidine has been measured simultaneously in plasma and urine in 20 healthy human volunteers by a specific G.L.C. method, after single and multiple oral dral doses of procainamide retard tablets. A distinct bimodality (9 rapid and 11 slow acetylators) was apparent from the concentrations of procainamide and N-acetylprocainamide both in urine and plasma, which was in complete agreement with data about sulfadimidine acetylation. The influence of acetylator phenotype on the relative concentrations of procainamide and N-acetylprocainamide in plasma as cn 5 additional healthy subjects after a single oral dose of procainamide. The present results show that acetylator phenotype can now be determined using procainamide as the test substance, and for this purpose multiple doses offer hardly any advantage over a single dose of the drug. However, because the separation between rapid and slow acetylators is less pronounced for procainamide than for sulfadimidine, precise criteria must be established for the conditions of the test, and the influence of diseases, such as renal insufficiency, should be taken into consideration.", "contents": "Comparison of the acetylation of procainamide and sulfadimidine in man. The acetylation of procainamide and sulfadimidine has been measured simultaneously in plasma and urine in 20 healthy human volunteers by a specific G.L.C. method, after single and multiple oral dral doses of procainamide retard tablets. A distinct bimodality (9 rapid and 11 slow acetylators) was apparent from the concentrations of procainamide and N-acetylprocainamide both in urine and plasma, which was in complete agreement with data about sulfadimidine acetylation. The influence of acetylator phenotype on the relative concentrations of procainamide and N-acetylprocainamide in plasma as cn 5 additional healthy subjects after a single oral dose of procainamide. The present results show that acetylator phenotype can now be determined using procainamide as the test substance, and for this purpose multiple doses offer hardly any advantage over a single dose of the drug. However, because the separation between rapid and slow acetylators is less pronounced for procainamide than for sulfadimidine, precise criteria must be established for the conditions of the test, and the influence of diseases, such as renal insufficiency, should be taken into consideration.", "PMID": 971707}
{"id": "pubmed23n0032_8645", "title": "The influence of disulfiram on the half life and metabolic clearance rate of diphenylhydantoin and tolbtamide in man.", "content": "Diphenylhydantoin (DPH) and tolbutamide serum levels were studied in ten volunteers before and after 4 days of disulfiram treatment. The mean DPH half life increased significantly from 11.0 +/- a.2 h to 19.0 +/- 3.3 h, and the mean DPH metabolic clearance rate decreased significantly from 51.2 +/- 17.2 ml/min to 33.9 +/- 12.0 ml/min during medication. No significant changes in the half life or metabolic clearance rate of tolbutamide was observed.", "contents": "The influence of disulfiram on the half life and metabolic clearance rate of diphenylhydantoin and tolbtamide in man. Diphenylhydantoin (DPH) and tolbutamide serum levels were studied in ten volunteers before and after 4 days of disulfiram treatment. The mean DPH half life increased significantly from 11.0 +/- a.2 h to 19.0 +/- 3.3 h, and the mean DPH metabolic clearance rate decreased significantly from 51.2 +/- 17.2 ml/min to 33.9 +/- 12.0 ml/min during medication. No significant changes in the half life or metabolic clearance rate of tolbutamide was observed.", "PMID": 971708}
{"id": "pubmed23n0032_8646", "title": "Measurement of plasma levels of tricylic psychoactive drugs and their metabolites by UV reflectance photometry of thin layer chromatograms.", "content": "A method has been developed for the determination of perazine, clozapine, imipramine and amitriptyline and their demethylated metabolites in plasma. Other metabolites measured were perazine sulfoxide and the N-oxides of clozapine and perazine, the latter two following their reduction to the parent drugs with ascorbic acid. 10-Hydroxynortriptyline was identified as an amitriptyline metabolite in plasma. The general procedure included extraction of alkalinized plasma samples (3 - 6 g) with benzene or toluene and thin layer chromatography of the extracts, followed by reflectance photometry of the plates at appropriate wave lengths in ultraviolet light. Spots of questionable identity were further characterized by two-dimensional chromatography and by colour reactions. Therecoveries of compounds added in therapeutic concentrations were between 70 and 98 %. The limits of detectability were 5 - 10 ng/g plasma.", "contents": "Measurement of plasma levels of tricylic psychoactive drugs and their metabolites by UV reflectance photometry of thin layer chromatograms. A method has been developed for the determination of perazine, clozapine, imipramine and amitriptyline and their demethylated metabolites in plasma. Other metabolites measured were perazine sulfoxide and the N-oxides of clozapine and perazine, the latter two following their reduction to the parent drugs with ascorbic acid. 10-Hydroxynortriptyline was identified as an amitriptyline metabolite in plasma. The general procedure included extraction of alkalinized plasma samples (3 - 6 g) with benzene or toluene and thin layer chromatography of the extracts, followed by reflectance photometry of the plates at appropriate wave lengths in ultraviolet light. Spots of questionable identity were further characterized by two-dimensional chromatography and by colour reactions. Therecoveries of compounds added in therapeutic concentrations were between 70 and 98 %. The limits of detectability were 5 - 10 ng/g plasma.", "PMID": 971709}
{"id": "pubmed23n0032_8647", "title": "Effect of aldosterone antagonist canrenone on plasma aldosterone concentration and plasma renin activity, and on the excretion of aldosterone and electrolytes by man.", "content": "Canrenone was administered in doses of 2 x 82 mg and 2 x 164 mg per day over a period of 10 days to diabetic patients without cardiovascular, liver or kidney involvement. Aldosterone excretion and plasma aldosterone increased only slightly during both regimes. There was a clear-cut increase in aldosterone excretion only after discontinuation of canrenone. Excretion of sodium, potassium and fluid was not significantly changed either during or after treatment. The lack of effect of canrenone on the kidney was in contrast to the significant decrease in serum sodium and increase in serum potassium, and the significant, dose-dependent rise in plasma renin activity following canrenone administration. The increased plasma renin activity persisted for some days after discontinuation of canrenone. It is suggested that canrenone primarily exerted its effect in the distal part of the large intestine where ionic movements are most affected by aldosterone. The disproportionately slight increase in plasma aldosterone concentration and aldosterone excretion, in spite of the greatly elevated plasma renin activity and serum potassium level, is considered to be due to a direct inhibitory effect of canrenone on aldosterone production in the adrenals.", "contents": "Effect of aldosterone antagonist canrenone on plasma aldosterone concentration and plasma renin activity, and on the excretion of aldosterone and electrolytes by man. Canrenone was administered in doses of 2 x 82 mg and 2 x 164 mg per day over a period of 10 days to diabetic patients without cardiovascular, liver or kidney involvement. Aldosterone excretion and plasma aldosterone increased only slightly during both regimes. There was a clear-cut increase in aldosterone excretion only after discontinuation of canrenone. Excretion of sodium, potassium and fluid was not significantly changed either during or after treatment. The lack of effect of canrenone on the kidney was in contrast to the significant decrease in serum sodium and increase in serum potassium, and the significant, dose-dependent rise in plasma renin activity following canrenone administration. The increased plasma renin activity persisted for some days after discontinuation of canrenone. It is suggested that canrenone primarily exerted its effect in the distal part of the large intestine where ionic movements are most affected by aldosterone. The disproportionately slight increase in plasma aldosterone concentration and aldosterone excretion, in spite of the greatly elevated plasma renin activity and serum potassium level, is considered to be due to a direct inhibitory effect of canrenone on aldosterone production in the adrenals.", "PMID": 971711}
{"id": "pubmed23n0032_8648", "title": "Theoretical analysis of the binding of salicylate by human serum albumin: the relationship between free and bound drug and therapeutic levels.", "content": "The binding of salicylates by human serum albumin was analyzed by use of a computer program using previously published association constants and binding capacities for the two sets of binding sites on the protein. The analysis consisted of computing free and bound salicylates for a range of therapeutic and toxic concentration from 181 to 7246 mumole/L (25 to 1000 mg/L). At low and therapeutic levels the total amount of bound drug would exceed the amount of free drug. At higher levels, which included therapeutic and toxic ranges, the amount of free drug plasma, up to 2000 mumole/L the high affinity sites (Site 1), would bind most of the drug, but as the concentration of drug increased this site would approach saturation and the low affinity Site 2 would bind increasing amounts of salicylate. At high salicylate levels the amount of drug bound by the low affinity sites. Computation also showed that when the total amount of protein in the analysis was reduced, from 5, 4, 3, to 2 gm%, as in hypoalbuminemia, the total amount of drug bound by the protein would decrease and the quantity of free drug would increase. The amount of drug bound by each of the two sets of sites also fell as the concentration of protein decreased. Some of the possible clinical implications of these findings are discussed.", "contents": "Theoretical analysis of the binding of salicylate by human serum albumin: the relationship between free and bound drug and therapeutic levels. The binding of salicylates by human serum albumin was analyzed by use of a computer program using previously published association constants and binding capacities for the two sets of binding sites on the protein. The analysis consisted of computing free and bound salicylates for a range of therapeutic and toxic concentration from 181 to 7246 mumole/L (25 to 1000 mg/L). At low and therapeutic levels the total amount of bound drug would exceed the amount of free drug. At higher levels, which included therapeutic and toxic ranges, the amount of free drug plasma, up to 2000 mumole/L the high affinity sites (Site 1), would bind most of the drug, but as the concentration of drug increased this site would approach saturation and the low affinity Site 2 would bind increasing amounts of salicylate. At high salicylate levels the amount of drug bound by the low affinity sites. Computation also showed that when the total amount of protein in the analysis was reduced, from 5, 4, 3, to 2 gm%, as in hypoalbuminemia, the total amount of drug bound by the protein would decrease and the quantity of free drug would increase. The amount of drug bound by each of the two sets of sites also fell as the concentration of protein decreased. Some of the possible clinical implications of these findings are discussed.", "PMID": 971712}
{"id": "pubmed23n0032_8649", "title": "Pharmacokinetics of lofepramine in man: relationship to inhibition of noradrenaline uptake.", "content": "The pharmacokinetics of lofepramine, an imipramine analogue, have been studied by administering single oral doses to volunteers, determination of plasma levels of lofepramine and desemthylimipramine after ten days of oral administration to patients, and by relating plasma levels to the effect on uptake of noradrenaline by isolated rats irides and brain slices of plasma samples collected during treatment. The results indicate that lofepramine undergoes pronounced first pass elimination and that desmethylimipramine is a major metabolite of it. During steady-state conditions the plasma level of lofepramine fluctuates considerably between doses. A linear relation was found between inhibition of neuronal uptake of noradrenaline and the plasma concentration of desmethylimipramine. No effect was seen on the uptake of 5-hydroxytryptamine in brain slices incubated in patients' plasma which suggests that neither lofepramine nor its metabolites formed in vivo in man affect neuronal uptake is this amine. Lofepramine belongs to the group of tricyclic antidepressants which preferentially inhibit noradrenaline uptake.", "contents": "Pharmacokinetics of lofepramine in man: relationship to inhibition of noradrenaline uptake. The pharmacokinetics of lofepramine, an imipramine analogue, have been studied by administering single oral doses to volunteers, determination of plasma levels of lofepramine and desemthylimipramine after ten days of oral administration to patients, and by relating plasma levels to the effect on uptake of noradrenaline by isolated rats irides and brain slices of plasma samples collected during treatment. The results indicate that lofepramine undergoes pronounced first pass elimination and that desmethylimipramine is a major metabolite of it. During steady-state conditions the plasma level of lofepramine fluctuates considerably between doses. A linear relation was found between inhibition of neuronal uptake of noradrenaline and the plasma concentration of desmethylimipramine. No effect was seen on the uptake of 5-hydroxytryptamine in brain slices incubated in patients' plasma which suggests that neither lofepramine nor its metabolites formed in vivo in man affect neuronal uptake is this amine. Lofepramine belongs to the group of tricyclic antidepressants which preferentially inhibit noradrenaline uptake.", "PMID": 971713}
{"id": "pubmed23n0032_8650", "title": "Absorption of beta-methyl-digoxin determined after a single dose and under steady state conditions.", "content": "Single doses of beta-methyl-digoxin 0.4 mg were given to groups of 17-18 healthy volunteers as an intravenous infusion lasting 2 hours, or orally as Lanitop Liquidum or Lanitop tablets. The serum glycoside concentration and urinary glycoside excretion were measured over 8 and 32 h. The absolute bioavailability from the oral preparations in comparison with the infusion was lower for the first 8 h than for the entire 32 h of the investigation; the relative bioavailability from tablets was the same as from the solution for both periods. For both periods the area under the serum concentration/time curve and the urinary glycoside excretion were significantly lower after administration of the tablets than after intravenous infusion. Taking the average of both parameters, the absolute bioavailability of beta-methyl-digoxin was about 80% from the solution and about 70% from the tablets. In 18 patients undergoing intravenous or oral therapy with beta-methyl-digoxin steady state glycoside concentration were compared in a cross-over study of intravenous maintenance therapy with Lanitop ampoules or oral treatment with Lanitop tablets. For a standard daily dose of 0.2 mg beta-methyl-digoxin the serum concentrations were 1.35 +/- 0.10 ng/ml during both intravenous and oral administration. The intra-individual variation in glycoside concentration after changing from intravenous to oral maintenance therapy, or vice versa, was about the same as during continued intravenous or oral administration. It is concluded that the rate of rise of serum concentration after a single dose may be a useful indicator of the rate of absorption, but that the area under the serum concentration/time curve and the urinary glycoside excretion up to 32 h are unsuitable for determining equivalent doses of different formulations or routes of administration of digitalis glycosides.", "contents": "Absorption of beta-methyl-digoxin determined after a single dose and under steady state conditions. Single doses of beta-methyl-digoxin 0.4 mg were given to groups of 17-18 healthy volunteers as an intravenous infusion lasting 2 hours, or orally as Lanitop Liquidum or Lanitop tablets. The serum glycoside concentration and urinary glycoside excretion were measured over 8 and 32 h. The absolute bioavailability from the oral preparations in comparison with the infusion was lower for the first 8 h than for the entire 32 h of the investigation; the relative bioavailability from tablets was the same as from the solution for both periods. For both periods the area under the serum concentration/time curve and the urinary glycoside excretion were significantly lower after administration of the tablets than after intravenous infusion. Taking the average of both parameters, the absolute bioavailability of beta-methyl-digoxin was about 80% from the solution and about 70% from the tablets. In 18 patients undergoing intravenous or oral therapy with beta-methyl-digoxin steady state glycoside concentration were compared in a cross-over study of intravenous maintenance therapy with Lanitop ampoules or oral treatment with Lanitop tablets. For a standard daily dose of 0.2 mg beta-methyl-digoxin the serum concentrations were 1.35 +/- 0.10 ng/ml during both intravenous and oral administration. The intra-individual variation in glycoside concentration after changing from intravenous to oral maintenance therapy, or vice versa, was about the same as during continued intravenous or oral administration. It is concluded that the rate of rise of serum concentration after a single dose may be a useful indicator of the rate of absorption, but that the area under the serum concentration/time curve and the urinary glycoside excretion up to 32 h are unsuitable for determining equivalent doses of different formulations or routes of administration of digitalis glycosides.", "PMID": 971714}
{"id": "pubmed23n0032_8651", "title": "Interindividual differences in chlorthalidone concentration in plasma and red cells of man after single and multiple doses.", "content": "A gas chromatographic method has been employed to determine chlorthalidone in plasma and whole blood after therapeutic doses. Radioactively labelled chlorthalidone was used for in vitro studies of the uptake of chlorthalidone from plasma by red blood cells. Chlorthalidone was markedly concentrated in red cells and as a compartment they would account for at least 30% of total drug in the body after multiple doses. The ratio between the plasma and red cell concentration of chlorathidone varied between individuals. After a single oral dose of 50 mg in 6 healthy volunteers chlorthalidone was eliminated with a half-life of 51 to 89 hours. The apparent volume of distribution varied between 3 and 13 1/kg and the clearance between 53 and 145 ml/min. The mean steady-state plasma concentrations during treatment with a standard dose of 50 mg daily (n = 10) varied 5-fold between individuals. During the steady state approximately 50% of the daily dose was excreted unchanged in the urine during 24 hrs. The plasma levels observed in patients were higher than those preducted from the single oral dose studies in healthy volunteers.", "contents": "Interindividual differences in chlorthalidone concentration in plasma and red cells of man after single and multiple doses. A gas chromatographic method has been employed to determine chlorthalidone in plasma and whole blood after therapeutic doses. Radioactively labelled chlorthalidone was used for in vitro studies of the uptake of chlorthalidone from plasma by red blood cells. Chlorthalidone was markedly concentrated in red cells and as a compartment they would account for at least 30% of total drug in the body after multiple doses. The ratio between the plasma and red cell concentration of chlorathidone varied between individuals. After a single oral dose of 50 mg in 6 healthy volunteers chlorthalidone was eliminated with a half-life of 51 to 89 hours. The apparent volume of distribution varied between 3 and 13 1/kg and the clearance between 53 and 145 ml/min. The mean steady-state plasma concentrations during treatment with a standard dose of 50 mg daily (n = 10) varied 5-fold between individuals. During the steady state approximately 50% of the daily dose was excreted unchanged in the urine during 24 hrs. The plasma levels observed in patients were higher than those preducted from the single oral dose studies in healthy volunteers.", "PMID": 971715}
{"id": "pubmed23n0032_8652", "title": "Comparison of the half-life of antipyrine in plasma, whole blood and saliva of man.", "content": "A previously described GLC method has been modified and applied to measurement of antipyrine levels in plasma, blood and saliva of man following administration of a single oral dose (10 mg/kg). Tery time studied. The half-life of antipyrine determined in blood, plasma or saliva in any given individual was similar. The intersubject variation in half-life was about two-fold (n = 5). Antipyrine levels in saliva were not affected by the rate of saliva flow when collections were made continuously for 20 minutes. This study has demonstrated that kinetic data about antipyrine comparable to that from plasma may also be obtained from readily accessible tissue fluids, such as saliva  and capillary blood.", "contents": "Comparison of the half-life of antipyrine in plasma, whole blood and saliva of man. A previously described GLC method has been modified and applied to measurement of antipyrine levels in plasma, blood and saliva of man following administration of a single oral dose (10 mg/kg). Tery time studied. The half-life of antipyrine determined in blood, plasma or saliva in any given individual was similar. The intersubject variation in half-life was about two-fold (n = 5). Antipyrine levels in saliva were not affected by the rate of saliva flow when collections were made continuously for 20 minutes. This study has demonstrated that kinetic data about antipyrine comparable to that from plasma may also be obtained from readily accessible tissue fluids, such as saliva  and capillary blood.", "PMID": 971716}
{"id": "pubmed23n0032_8653", "title": "Membrane Ig on MPC11 myeloma cells: correlation between the expression of membrane Ig, a receptor for Ig and the process of secretion.", "content": "The expression of membrane immunoglobulin (mIg) was examined in three cloned MPC11-derived mouse myeloma cell lines. Membrane immunofluorescence studies demonstrated that IgG2b producer cells (P1) had complete IgG molecules, L-chain producer (L1) had only L-chain determinants and nonproducer (NP2) did not have any Ig determinants on the cell surface. An Ig receptor, with characteristics different from B lymphocyte Fc receptor, has been found to be present on secreting cells (P1 or L1), but not on the NP2 cell variant. The data reported in the present paper indicate that the expression of mIg and of the Ig receptor molecule is clearly correlated with the process of secretion. In the light of previous data reported on Ig secretion, a model is proposed which correlates the process of secretion with the expression not only of mIg, but also of the receptor for Ig.", "contents": "Membrane Ig on MPC11 myeloma cells: correlation between the expression of membrane Ig, a receptor for Ig and the process of secretion. The expression of membrane immunoglobulin (mIg) was examined in three cloned MPC11-derived mouse myeloma cell lines. Membrane immunofluorescence studies demonstrated that IgG2b producer cells (P1) had complete IgG molecules, L-chain producer (L1) had only L-chain determinants and nonproducer (NP2) did not have any Ig determinants on the cell surface. An Ig receptor, with characteristics different from B lymphocyte Fc receptor, has been found to be present on secreting cells (P1 or L1), but not on the NP2 cell variant. The data reported in the present paper indicate that the expression of mIg and of the Ig receptor molecule is clearly correlated with the process of secretion. In the light of previous data reported on Ig secretion, a model is proposed which correlates the process of secretion with the expression not only of mIg, but also of the receptor for Ig.", "PMID": 971717}
{"id": "pubmed23n0032_8654", "title": "Immunological enhancement of a murine tumor allograft by passive alloantibody IgG and F(ab')2.", "content": "The F(ab')2 fraction of an anti-tumor IgG alloantiserum induced passive enhancement of tumor allografts in inbred mice about as well as did intact IgG; i.e. apparently the Fc fragment is not required for suppressing cellular immunity. Passive F(ab')2, however, was somewhat less efficient than intact IgG or whole alloantiserum in depressing the allohemagglutinin response. The latter finding is in accord with previous observations on the relative inefficiency of F(ab')2 in feedback inhibition of the humoral response of mice to a heteroantigen.", "contents": "Immunological enhancement of a murine tumor allograft by passive alloantibody IgG and F(ab')2. The F(ab')2 fraction of an anti-tumor IgG alloantiserum induced passive enhancement of tumor allografts in inbred mice about as well as did intact IgG; i.e. apparently the Fc fragment is not required for suppressing cellular immunity. Passive F(ab')2, however, was somewhat less efficient than intact IgG or whole alloantiserum in depressing the allohemagglutinin response. The latter finding is in accord with previous observations on the relative inefficiency of F(ab')2 in feedback inhibition of the humoral response of mice to a heteroantigen.", "PMID": 971718}
{"id": "pubmed23n0032_8655", "title": "Polygenic regulation of antibody synthesis to sheep erythrocytes in the mouse: a genetic analysis.", "content": "Selective breeding for the character \"agglutinin production to heterologous erythrocytes\" was performed on random-bred populations of albino mice. Two types of selection were carried out: Selection I: the first 6 generations were immunized with sheep erythrocytes (SE), then SE and pigeon erythrocytes (PE) were alternated at each generation to avoid the interference of maternal antibody. Selection II: all generations were immunized with SE 60-70 days after weaning in order to eliminate the maternal antibody. Both were selected for the character agglutinin titer 14 days after immunization using an optimal dose of erythrocytes. Selection I. The mean response to SE of the foundation population was 7.36 +/- 1.60. The selection limit was attained between the 15th and the 20th generation. The F20--F30 were considered as homozygous for the character investigated. The mean SE agglutinin titers were 9.15 (1/2820) in the high responder line and 2 (1/20) in the low responder line. This corresponds to 140-fold interline difference in agglutinin titers. SE agglutinins determined in F1 hybrids, F2 hybrids and backcrosses obtained from F23--F29 demonstrated incomplete dominance of high responsiveness (23-29%). The inbreeding coefficient produced by close colony breeding was 0.55 in high and 0.71 in low responders at the 20th generation when both lines were homozygous with respect to agglutinin synthesis. Selection II. The mean response of the foundation population to SE was 7.79 +/- 1.56. The selection limit was attained in F14--F17 generations that were considered homozygous for the character investigated. The mean SE agglutinin titer was 9.2 (1/2900) in high responders and 2.8 (1/35) in low responders, which is an 83-fold interline difference in agglutinin titers. The results of both selections indicate that the character agglutinin synthesis is subject to polygenic regulation. The heritability (h2) of this character was estimated to be between 0.18 and 0.36 (range between the extreme values of Selections I and II.", "contents": "Polygenic regulation of antibody synthesis to sheep erythrocytes in the mouse: a genetic analysis. Selective breeding for the character \"agglutinin production to heterologous erythrocytes\" was performed on random-bred populations of albino mice. Two types of selection were carried out: Selection I: the first 6 generations were immunized with sheep erythrocytes (SE), then SE and pigeon erythrocytes (PE) were alternated at each generation to avoid the interference of maternal antibody. Selection II: all generations were immunized with SE 60-70 days after weaning in order to eliminate the maternal antibody. Both were selected for the character agglutinin titer 14 days after immunization using an optimal dose of erythrocytes. Selection I. The mean response to SE of the foundation population was 7.36 +/- 1.60. The selection limit was attained between the 15th and the 20th generation. The F20--F30 were considered as homozygous for the character investigated. The mean SE agglutinin titers were 9.15 (1/2820) in the high responder line and 2 (1/20) in the low responder line. This corresponds to 140-fold interline difference in agglutinin titers. SE agglutinins determined in F1 hybrids, F2 hybrids and backcrosses obtained from F23--F29 demonstrated incomplete dominance of high responsiveness (23-29%). The inbreeding coefficient produced by close colony breeding was 0.55 in high and 0.71 in low responders at the 20th generation when both lines were homozygous with respect to agglutinin synthesis. Selection II. The mean response of the foundation population to SE was 7.79 +/- 1.56. The selection limit was attained in F14--F17 generations that were considered homozygous for the character investigated. The mean SE agglutinin titer was 9.2 (1/2900) in high responders and 2.8 (1/35) in low responders, which is an 83-fold interline difference in agglutinin titers. The results of both selections indicate that the character agglutinin synthesis is subject to polygenic regulation. The heritability (h2) of this character was estimated to be between 0.18 and 0.36 (range between the extreme values of Selections I and II.", "PMID": 971719}
{"id": "pubmed23n0032_8656", "title": "Relationship between the enhanced expression of histocompatibility antigens on interferon-treated L 1210 cells and their position in the cell cycle.", "content": "We have determined the cell cycle position of individual mouse leukemia L 1210 cells by cytophotometric and autoradiographic techniques and simultaneously determined the amount of histocompatibility antigens expressed on the surface of individual cells by quantitative immunofluorescence. Interferon treatment of L 1210 cells was accompanied by an enhanced expression of histocompatibility surface antigens. The distribution of interferon-treated cells in the various phases of the cell cycle was similar to that for control cells and the enhanced expression of histocompatibility antigens was observed on interferon-treated cells in all phases of the cell cycle. We conclude, therefore, that this enhancement is not due to a preferential concentration of these cells in any one particular phase of the cell cycle.", "contents": "Relationship between the enhanced expression of histocompatibility antigens on interferon-treated L 1210 cells and their position in the cell cycle. We have determined the cell cycle position of individual mouse leukemia L 1210 cells by cytophotometric and autoradiographic techniques and simultaneously determined the amount of histocompatibility antigens expressed on the surface of individual cells by quantitative immunofluorescence. Interferon treatment of L 1210 cells was accompanied by an enhanced expression of histocompatibility surface antigens. The distribution of interferon-treated cells in the various phases of the cell cycle was similar to that for control cells and the enhanced expression of histocompatibility antigens was observed on interferon-treated cells in all phases of the cell cycle. We conclude, therefore, that this enhancement is not due to a preferential concentration of these cells in any one particular phase of the cell cycle.", "PMID": 971720}
{"id": "pubmed23n0032_8657", "title": "Reversible binding of a T cell factor on IgG-coated sepharose beads.", "content": "Supernatants of alloantigen-activated T cells contain a number of factors, including an immunoglobulin-binding factor (IBF) which inhibits complement-induced hemolysis of sheep erythrocytes coated with anti-Forssman IgG antibodies and a factor which suppresses IgM antibody synthesis in vitro. These two factors may be identical, since they are simultaneously retained on Sepharose beads to which IgG has been coupled and can be recovered by elution at pH 2.8. They do not bind to Sepharose beads to which IgM of F(ab')2 fragment of IgG has been coupled, demonstrating that they have a selective affinity for the Fc region of IgG. In addition, the fixation of IBF on the Fc portion of IgG reversibly inhibits subsequent binding of the first component of complement (C1), thus indicating that IBF does not irreversibly alter the C1 binding site(s) of IgG.", "contents": "Reversible binding of a T cell factor on IgG-coated sepharose beads. Supernatants of alloantigen-activated T cells contain a number of factors, including an immunoglobulin-binding factor (IBF) which inhibits complement-induced hemolysis of sheep erythrocytes coated with anti-Forssman IgG antibodies and a factor which suppresses IgM antibody synthesis in vitro. These two factors may be identical, since they are simultaneously retained on Sepharose beads to which IgG has been coupled and can be recovered by elution at pH 2.8. They do not bind to Sepharose beads to which IgM of F(ab')2 fragment of IgG has been coupled, demonstrating that they have a selective affinity for the Fc region of IgG. In addition, the fixation of IBF on the Fc portion of IgG reversibly inhibits subsequent binding of the first component of complement (C1), thus indicating that IBF does not irreversibly alter the C1 binding site(s) of IgG.", "PMID": 971721}
{"id": "pubmed23n0032_8658", "title": "Synthesis of eel-calcitonin and (asu1,7)-eel-calcitonin: contribution of the disulfide bond to the hormonal activity.", "content": "Eel-calcitonin and its [asu1,7]-analog, deamino-dicarba-analog, were synthesized by the conventional solution method. The natural-type product showed 4300 MRC U/mg in the hypocalcemic potency which was comparable to that of the natural hormone isolated from eel. Hormonal activity of the Asu-analog was also as high as 3400 MRC U/mg.", "contents": "Synthesis of eel-calcitonin and (asu1,7)-eel-calcitonin: contribution of the disulfide bond to the hormonal activity. Eel-calcitonin and its [asu1,7]-analog, deamino-dicarba-analog, were synthesized by the conventional solution method. The natural-type product showed 4300 MRC U/mg in the hypocalcemic potency which was comparable to that of the natural hormone isolated from eel. Hormonal activity of the Asu-analog was also as high as 3400 MRC U/mg.", "PMID": 971732}
{"id": "pubmed23n0032_8659", "title": "Phosphorus-containing heterocycles as fungicides: synthesis of 2,2'-diphenylene chlorophosphonate and 2,2'-diphenylene chlorothiophosphonate.", "content": "Synthesis of 2,2'-diphenylene chlorophosphonate and 2,2'-diphenylene chlorothiophosphonate is described through the interaction of 2,2'-dihydroxybiphenyl with phosphoryl chloride and thiophosphoryl chloride respectively. These compounds were screened for their fungicidal activity.", "contents": "Phosphorus-containing heterocycles as fungicides: synthesis of 2,2'-diphenylene chlorophosphonate and 2,2'-diphenylene chlorothiophosphonate. Synthesis of 2,2'-diphenylene chlorophosphonate and 2,2'-diphenylene chlorothiophosphonate is described through the interaction of 2,2'-dihydroxybiphenyl with phosphoryl chloride and thiophosphoryl chloride respectively. These compounds were screened for their fungicidal activity.", "PMID": 971733}
{"id": "pubmed23n0032_8660", "title": "On the metabolism of prostaglandins by rat brain homogenate.", "content": "Using radio-immuno assays for prostaglandins and prostaglandin metabolites, three prostaglandin metabolizing enzymes were found in the 100,000 X g supernatant of rat brain, 15-hydroxy-prostaglandin-dehydrogenase, delta13-reductase and prostaglandin E-9-keto-reductase. Specific activity of the latter enzyme was highest in striatum and midbrain and lowest in cortex, cerebellum and spinal cord.", "contents": "On the metabolism of prostaglandins by rat brain homogenate. Using radio-immuno assays for prostaglandins and prostaglandin metabolites, three prostaglandin metabolizing enzymes were found in the 100,000 X g supernatant of rat brain, 15-hydroxy-prostaglandin-dehydrogenase, delta13-reductase and prostaglandin E-9-keto-reductase. Specific activity of the latter enzyme was highest in striatum and midbrain and lowest in cortex, cerebellum and spinal cord.", "PMID": 971735}
{"id": "pubmed23n0032_8661", "title": "Changes in blood tryptophan level during sleep deprivation.", "content": "Prolonged sleep deprivation elicits a significant elevation of the plasma level of free tryptophan which appears to be involved in increased excretion of 5-HIAA during this state through enhanced 5-HT synthesis.", "contents": "Changes in blood tryptophan level during sleep deprivation. Prolonged sleep deprivation elicits a significant elevation of the plasma level of free tryptophan which appears to be involved in increased excretion of 5-HIAA during this state through enhanced 5-HT synthesis.", "PMID": 971736}
{"id": "pubmed23n0032_8662", "title": "Purification and partial characterization of lysozyme from mouse small intestine.", "content": "Lysozyme was isolated from the small intestine of mice by combined ion-exchange and molecular sieve chromatography. This lysozyme differs from that isolated from the urine of mice with monocytoma in amino acid composition, and migration rate in cellulose acetate electrophoresis. As intestinal lysozyme originates at least in part from the Paneth cell, our results point towards the existence of isozymes of lysozymes in mice.", "contents": "Purification and partial characterization of lysozyme from mouse small intestine. Lysozyme was isolated from the small intestine of mice by combined ion-exchange and molecular sieve chromatography. This lysozyme differs from that isolated from the urine of mice with monocytoma in amino acid composition, and migration rate in cellulose acetate electrophoresis. As intestinal lysozyme originates at least in part from the Paneth cell, our results point towards the existence of isozymes of lysozymes in mice.", "PMID": 971738}
{"id": "pubmed23n0032_8663", "title": "Galactose transfer and UDP-galactose hydrolysis in urine from normal Balb/c and Balb/c YC8 mice.", "content": "By chromatographic method we have shown the existence of a complex system for galactose transfer from UDP-galactose and for nucleotide hydrolysis in urines from Balb/c YC8 and normal Balb/c mice. By action of sera from normal and ascitic mice as source of enzyme, we have been able to detect transfer for galactose in urines from ascitic mice and an important inhibitory effect of the nucleotide sugar hydrolysis by the sera with urines from normal mice.", "contents": "Galactose transfer and UDP-galactose hydrolysis in urine from normal Balb/c and Balb/c YC8 mice. By chromatographic method we have shown the existence of a complex system for galactose transfer from UDP-galactose and for nucleotide hydrolysis in urines from Balb/c YC8 and normal Balb/c mice. By action of sera from normal and ascitic mice as source of enzyme, we have been able to detect transfer for galactose in urines from ascitic mice and an important inhibitory effect of the nucleotide sugar hydrolysis by the sera with urines from normal mice.", "PMID": 971739}
{"id": "pubmed23n0032_8664", "title": "Monoamine oxidase activity in rat erythrocytes: evidence for its localization in reticulocyte mitrochondria.", "content": "During growth of young rats, monoamine oxidase activity in erythrocyte membrane preparations decreases more rapidly than reticulocyte concentrations in the respective blood samples. Since reticulocytes lose their mitochondria prior to the substantia reticulo-filamentosa, the non-linear correlation between monoamine oxidase activity and reticulocyte counts indicates that erythrocyte monoamine oxidase is located in reticulocyte mitochondria.", "contents": "Monoamine oxidase activity in rat erythrocytes: evidence for its localization in reticulocyte mitrochondria. During growth of young rats, monoamine oxidase activity in erythrocyte membrane preparations decreases more rapidly than reticulocyte concentrations in the respective blood samples. Since reticulocytes lose their mitochondria prior to the substantia reticulo-filamentosa, the non-linear correlation between monoamine oxidase activity and reticulocyte counts indicates that erythrocyte monoamine oxidase is located in reticulocyte mitochondria.", "PMID": 971741}
{"id": "pubmed23n0032_8665", "title": "The oxidative metabolism of alpha-chlorohydrin and the chemical induction of spermatocoeles.", "content": "alpha-Chlorohydrin (I) is oxidatively metabolized to beta-chlorolactic acid (III) and oxalic acid (IV). Deposition of calcium oxalate within the renal tubules is responsible for the toxic effects of alpha-chlorohydrin and a similar action on the epididymis or epididymal blood vessels could initiate the formation of spermatocoeles from this and other male antifertility agents.", "contents": "The oxidative metabolism of alpha-chlorohydrin and the chemical induction of spermatocoeles. alpha-Chlorohydrin (I) is oxidatively metabolized to beta-chlorolactic acid (III) and oxalic acid (IV). Deposition of calcium oxalate within the renal tubules is responsible for the toxic effects of alpha-chlorohydrin and a similar action on the epididymis or epididymal blood vessels could initiate the formation of spermatocoeles from this and other male antifertility agents.", "PMID": 971742}
{"id": "pubmed23n0032_8666", "title": "Developmental characteristics of histamine methyltransferase and phenylethanolamine-N-methyl-transferase of rat brain.", "content": "The specific activity of histamine methyltransferase of rat brain increases rapidly from the 16th until the 25th day of gestation (7 days after birth). The specific activity of phenylethanolamine-N-methyl-transferase shows a rapid increase during the 1st and the 2nd week after birth, the adult values being obtained by the end of the 2nd week.", "contents": "Developmental characteristics of histamine methyltransferase and phenylethanolamine-N-methyl-transferase of rat brain. The specific activity of histamine methyltransferase of rat brain increases rapidly from the 16th until the 25th day of gestation (7 days after birth). The specific activity of phenylethanolamine-N-methyl-transferase shows a rapid increase during the 1st and the 2nd week after birth, the adult values being obtained by the end of the 2nd week.", "PMID": 971743}
{"id": "pubmed23n0032_8667", "title": "Alteration of cholesterol synthesis in rat liver as induced by 4-methyl-5-hydroxy valeric acid.", "content": "The rate of cholesterogenesis in rat liver, measured by the incorporation of labelled acetate or mevalonate into cholesterol, was significantly supressed by the use of 4-methyl-5-hydroxy valeric acid sodium salt. This effect cannot be explained by changes in HMG CoA reductase activity.", "contents": "Alteration of cholesterol synthesis in rat liver as induced by 4-methyl-5-hydroxy valeric acid. The rate of cholesterogenesis in rat liver, measured by the incorporation of labelled acetate or mevalonate into cholesterol, was significantly supressed by the use of 4-methyl-5-hydroxy valeric acid sodium salt. This effect cannot be explained by changes in HMG CoA reductase activity.", "PMID": 971744}
{"id": "pubmed23n0032_8668", "title": "Location of an eye mutant in the onion fly Hylemya antiqua Meigen using a pericentric chromosome inversion.", "content": "With the use of a pericentric inversion in chromosome 3, an eye color mutant in the onion fly was located in chromosome 3. No recombination occurs in males; 40.2% recombination was observed in females. This linkage through the male facilitates further cytogenetic research on structural aberrations involving chromosome 3.", "contents": "Location of an eye mutant in the onion fly Hylemya antiqua Meigen using a pericentric chromosome inversion. With the use of a pericentric inversion in chromosome 3, an eye color mutant in the onion fly was located in chromosome 3. No recombination occurs in males; 40.2% recombination was observed in females. This linkage through the male facilitates further cytogenetic research on structural aberrations involving chromosome 3.", "PMID": 971745}
{"id": "pubmed23n0032_8669", "title": "The phylogenetic status of phyllomedusine frogs (Hylidae) as evidenced from immunological studies of their serum albumins.", "content": "Based on immunological comparisons of the serum albumins of phyllomedusine frogs with both hyline and bufonid species, it is suggested that phyllomedusine frogs be erected to familial status within the superfamily Bufonoidea.", "contents": "The phylogenetic status of phyllomedusine frogs (Hylidae) as evidenced from immunological studies of their serum albumins. Based on immunological comparisons of the serum albumins of phyllomedusine frogs with both hyline and bufonid species, it is suggested that phyllomedusine frogs be erected to familial status within the superfamily Bufonoidea.", "PMID": 971747}
{"id": "pubmed23n0032_8670", "title": "Insulin resistance and related electrical activity of the small intestine.", "content": "Postprandial disruption of the migrating myoelectric complex is of longer duration in obese hyperinsulemic Zucker rats than in control rats. No postprandial disruption was seen in an insulin resistant dog. This gives further support to a major role for insulin in control of gut activity.", "contents": "Insulin resistance and related electrical activity of the small intestine. Postprandial disruption of the migrating myoelectric complex is of longer duration in obese hyperinsulemic Zucker rats than in control rats. No postprandial disruption was seen in an insulin resistant dog. This gives further support to a major role for insulin in control of gut activity.", "PMID": 971749}
{"id": "pubmed23n0032_8671", "title": "Ultradian and other rhythms in human respiration rate.", "content": "Twelve male subjects had their respiration rate (RR) measured at 3 min intervals for 6 h. Although there were substantial individual differences, most subjects demonstrated a 90 +/- 15 min rhythm of RR, with several subjects also showing 60 +/- 10 min and 30 +/- 3 min rhythms.", "contents": "Ultradian and other rhythms in human respiration rate. Twelve male subjects had their respiration rate (RR) measured at 3 min intervals for 6 h. Although there were substantial individual differences, most subjects demonstrated a 90 +/- 15 min rhythm of RR, with several subjects also showing 60 +/- 10 min and 30 +/- 3 min rhythms.", "PMID": 971750}
{"id": "pubmed23n0032_8672", "title": "Coordinated activation of muscle fibres by different conduction velocities in branches of a crustacean motor axon.", "content": "Higher conduction velocities in branches of the fast excitor axon to distal muscle fibres ensure that these fibres are activated almost simultaneously with proximal fibres in the claw closer muscle of lobsters, producing a contraction of maximal force.", "contents": "Coordinated activation of muscle fibres by different conduction velocities in branches of a crustacean motor axon. Higher conduction velocities in branches of the fast excitor axon to distal muscle fibres ensure that these fibres are activated almost simultaneously with proximal fibres in the claw closer muscle of lobsters, producing a contraction of maximal force.", "PMID": 971752}
{"id": "pubmed23n0032_8673", "title": "Effect of sulfated and non-sulfated gastrin and octapeptide-cholecystokinin on cat gall bladder in vitro.", "content": "This study demonstrates that for the isolated cat gall bladder a smaller molar dose of the sulfated form of OP-CCK and gastrin is required to produce contraction as compared to the respective non-sulfated forms. For OP the D50 for the sulfated form versus the non-sulfated form was 1.94. For gastrin it was 1.10.", "contents": "Effect of sulfated and non-sulfated gastrin and octapeptide-cholecystokinin on cat gall bladder in vitro. This study demonstrates that for the isolated cat gall bladder a smaller molar dose of the sulfated form of OP-CCK and gastrin is required to produce contraction as compared to the respective non-sulfated forms. For OP the D50 for the sulfated form versus the non-sulfated form was 1.94. For gastrin it was 1.10.", "PMID": 971753}
{"id": "pubmed23n0032_8674", "title": "Resistance of cold-exposed rats to aflatoxin.", "content": "Male rats kept at a temperature of 4 degrees-5 degrees C were refractory to a lethal dose of aflatoxin compared to animals at 20 degrees-21 degrees C which exhibited a high mortality and marked liver damage. It is suggested that this decreased susceptibility is mediated through a stimulated microsomal drug-metabolizing system in cold environment.", "contents": "Resistance of cold-exposed rats to aflatoxin. Male rats kept at a temperature of 4 degrees-5 degrees C were refractory to a lethal dose of aflatoxin compared to animals at 20 degrees-21 degrees C which exhibited a high mortality and marked liver damage. It is suggested that this decreased susceptibility is mediated through a stimulated microsomal drug-metabolizing system in cold environment.", "PMID": 971755}
{"id": "pubmed23n0032_8675", "title": "Enhancement of tubular organic base accumulation in renal cortical slices by repeated administrations of Tris-hydroxymethyl aminomethane, tromethamol (THAM) to rats of different ages.", "content": "After repeated THAM administrations to rats of different ages, an enhancement of THAM accumulation in renal cortical slices was observed, except in newborns. This effect can be interpreted as a specific substrate stimulation of the organic base transport system.", "contents": "Enhancement of tubular organic base accumulation in renal cortical slices by repeated administrations of Tris-hydroxymethyl aminomethane, tromethamol (THAM) to rats of different ages. After repeated THAM administrations to rats of different ages, an enhancement of THAM accumulation in renal cortical slices was observed, except in newborns. This effect can be interpreted as a specific substrate stimulation of the organic base transport system.", "PMID": 971756}
{"id": "pubmed23n0032_8676", "title": "RNA synthesis in alpha-amanitin-poisoned rats: prevention of recovery by inhibition of protein synthesis.", "content": "The treatment with cycloheximide of rats previously poisoned with alpha-amanitin hinders the recovery of RNA synthesis observed in the liver of rats treated with alpha-amanitin alone. The recovery of RNA synthesis can be ascribed to the capability of poisoned rats to synthesize new RNA-polymerase II.", "contents": "RNA synthesis in alpha-amanitin-poisoned rats: prevention of recovery by inhibition of protein synthesis. The treatment with cycloheximide of rats previously poisoned with alpha-amanitin hinders the recovery of RNA synthesis observed in the liver of rats treated with alpha-amanitin alone. The recovery of RNA synthesis can be ascribed to the capability of poisoned rats to synthesize new RNA-polymerase II.", "PMID": 971757}
{"id": "pubmed23n0032_8677", "title": "Hypertension mediated by the activation of the rat brain 5-hydroxytryptamine receptor sites.", "content": "5-Hydroxytryptamine (5-HT) administered intraventricularly (i.vent.) in rats produced hypertension without considerable changes in heart rate. After transsection of the spinal cord or i.vent. administration of methysergide, 5-HT failed to produce the pressor effect. Thus, the hypertension results from the activation of 5-HT receptor sites of the rat brain.", "contents": "Hypertension mediated by the activation of the rat brain 5-hydroxytryptamine receptor sites. 5-Hydroxytryptamine (5-HT) administered intraventricularly (i.vent.) in rats produced hypertension without considerable changes in heart rate. After transsection of the spinal cord or i.vent. administration of methysergide, 5-HT failed to produce the pressor effect. Thus, the hypertension results from the activation of 5-HT receptor sites of the rat brain.", "PMID": 971759}
{"id": "pubmed23n0032_8678", "title": "An electron microscopic study on the autonomic innervation of the rabbit parotid gland.", "content": "As visualized in the electron microscope, the parotid gland of the rabbit has a dual innervation. Both adrenergic and cholinergic nerves are equally distributed in the parenchyma and often run together within the same nerve bundle. Nerve terminals are observed not only subjacent to the basement membrane but interposed between the latter and the acinar cells, where they establish a close membrane to membrane contact with the latter.", "contents": "An electron microscopic study on the autonomic innervation of the rabbit parotid gland. As visualized in the electron microscope, the parotid gland of the rabbit has a dual innervation. Both adrenergic and cholinergic nerves are equally distributed in the parenchyma and often run together within the same nerve bundle. Nerve terminals are observed not only subjacent to the basement membrane but interposed between the latter and the acinar cells, where they establish a close membrane to membrane contact with the latter.", "PMID": 971761}
{"id": "pubmed23n0032_8679", "title": "The fine structure of Walker 256 carcinoma cells.", "content": "Several ultrastructural features of Walker 256 rat tumor cells which have not been observed before are revealed. Nuclear bodies either singlely or in pairs were found in some cells. Cytofilaments were observed in the cytoplasm and cytoplasmic projections in a few cells. Desmosomes which are usually regarded as a feature of differentiated cells were also observed.", "contents": "The fine structure of Walker 256 carcinoma cells. Several ultrastructural features of Walker 256 rat tumor cells which have not been observed before are revealed. Nuclear bodies either singlely or in pairs were found in some cells. Cytofilaments were observed in the cytoplasm and cytoplasmic projections in a few cells. Desmosomes which are usually regarded as a feature of differentiated cells were also observed.", "PMID": 971762}
{"id": "pubmed23n0032_8680", "title": "Pregnancy following segmental isthmic reversal of the rabbit oviduct.", "content": "Microsurgical reversal of a segment of rabbit proximal tubal isthmus has been followed by normal pregnency in the first two animals to undergo the procedure. Establishment of pregnancy despite radical modification of the oviduct furnishes the opportunity to gain new insights into the mechanisms controlling tubal ovum transport and emphasizes the evolving feasibility and importance of tuboplastic microsurgery both as a research tool and clinical procedure.", "contents": "Pregnancy following segmental isthmic reversal of the rabbit oviduct. Microsurgical reversal of a segment of rabbit proximal tubal isthmus has been followed by normal pregnency in the first two animals to undergo the procedure. Establishment of pregnancy despite radical modification of the oviduct furnishes the opportunity to gain new insights into the mechanisms controlling tubal ovum transport and emphasizes the evolving feasibility and importance of tuboplastic microsurgery both as a research tool and clinical procedure.", "PMID": 971763}
{"id": "pubmed23n0032_8681", "title": "Tumor promoting constituent of Euphorbia serrata L. latex.", "content": "Euphorbia serrata latex has initially ingenol-3-palmitate, which by action of silica gel is converted to ingenol-20-palmitate. The former is responsible for the irritant and cocarcinogenic activity of the latex on mouse ear and on mice back skin.", "contents": "Tumor promoting constituent of Euphorbia serrata L. latex. Euphorbia serrata latex has initially ingenol-3-palmitate, which by action of silica gel is converted to ingenol-20-palmitate. The former is responsible for the irritant and cocarcinogenic activity of the latex on mouse ear and on mice back skin.", "PMID": 971764}
{"id": "pubmed23n0032_8682", "title": "Pregnancy specific beta1-glycoprotein--a product of the syncytiotrophoblast.", "content": "Pregnancy specific beta1-glycoprotein (PSbetaG) has been identified in vitro in trophoblast cultures and in vivo, using transmission electron microscopy, in the syncytiotrophoblast, PSbetaG may, like other pregnancy proteins, have immunosuppressive properties.", "contents": "Pregnancy specific beta1-glycoprotein--a product of the syncytiotrophoblast. Pregnancy specific beta1-glycoprotein (PSbetaG) has been identified in vitro in trophoblast cultures and in vivo, using transmission electron microscopy, in the syncytiotrophoblast, PSbetaG may, like other pregnancy proteins, have immunosuppressive properties.", "PMID": 971765}
{"id": "pubmed23n0032_8683", "title": "Effect of an antiandrogen on the lymphoid system.", "content": "A side-effect of the administration of cyproterone acetate, an antiandrogenic steroid, to newborn, juvenile or adult male mice (in doses comparable to those used clinically) was found in a marked reduction of the white pulp of the spleen and reduced weight or even absence of the thymus.", "contents": "Effect of an antiandrogen on the lymphoid system. A side-effect of the administration of cyproterone acetate, an antiandrogenic steroid, to newborn, juvenile or adult male mice (in doses comparable to those used clinically) was found in a marked reduction of the white pulp of the spleen and reduced weight or even absence of the thymus.", "PMID": 971766}
{"id": "pubmed23n0032_8684", "title": "Progesterone formation and metabolism by rabbit placenta in vitro.", "content": "4-14C-Progesterone and 4-14C-pregnenolone are metabolized in vitro by rabbit placenta, at day 15 and 28 of gestation, exclusively to compounds reduced in ring A (5beta) and at carbon 3 and 20.", "contents": "Progesterone formation and metabolism by rabbit placenta in vitro. 4-14C-Progesterone and 4-14C-pregnenolone are metabolized in vitro by rabbit placenta, at day 15 and 28 of gestation, exclusively to compounds reduced in ring A (5beta) and at carbon 3 and 20.", "PMID": 971767}
{"id": "pubmed23n0032_8685", "title": "The underwater electro-olfactogram: a tool for the study of the sense of smell of marine fishes.", "content": "Recording the olfactory receptor activity of marine fishes presents problems due to the shunting of the electrical signals by the highly conductive sea water, which results in significant signal loss. By recording the large signal-to-noise ratio D. C. potentials using the underwater electro-olfactogram (EOG), we were able to study olfactory receptor properties of freshwater and marine fishes in a comparable manner.", "contents": "The underwater electro-olfactogram: a tool for the study of the sense of smell of marine fishes. Recording the olfactory receptor activity of marine fishes presents problems due to the shunting of the electrical signals by the highly conductive sea water, which results in significant signal loss. By recording the large signal-to-noise ratio D. C. potentials using the underwater electro-olfactogram (EOG), we were able to study olfactory receptor properties of freshwater and marine fishes in a comparable manner.", "PMID": 971769}
{"id": "pubmed23n0032_8686", "title": "The pituitary-gonadal axis in spinal cord injury.", "content": "Our study documented that serum levels of luteinizing hormone, follicle-stimulating hormone, testosterone, and estradiol in 14 of 15 males with spinal cord injury (SCI) were within the normal range of those of age-matched controls. One patient had gynecomastia with compensated gonadal insufficiency of undetermined etiology. Five males with SCI showed a normal rise in serum testosterone levels following stimulation with human chorionic gonadotropin. Testicular biopsy in one of two males with SCI showed spermatogenic arrest. One of the biopsies showed immunoglobulin G immunofluorescence around the seminiferous tubule; the other demonstrated immunoglobulin A immunofluorescence in the spermatogonia. These findings suggest that, if a high incidence of impaired spermatogenesis does occur in spinal cord-injured patients, it is unlikely to be secondary to hormonal imbalance.", "contents": "The pituitary-gonadal axis in spinal cord injury. Our study documented that serum levels of luteinizing hormone, follicle-stimulating hormone, testosterone, and estradiol in 14 of 15 males with spinal cord injury (SCI) were within the normal range of those of age-matched controls. One patient had gynecomastia with compensated gonadal insufficiency of undetermined etiology. Five males with SCI showed a normal rise in serum testosterone levels following stimulation with human chorionic gonadotropin. Testicular biopsy in one of two males with SCI showed spermatogenic arrest. One of the biopsies showed immunoglobulin G immunofluorescence around the seminiferous tubule; the other demonstrated immunoglobulin A immunofluorescence in the spermatogonia. These findings suggest that, if a high incidence of impaired spermatogenesis does occur in spinal cord-injured patients, it is unlikely to be secondary to hormonal imbalance.", "PMID": 971771}
{"id": "pubmed23n0032_8687", "title": "Sexual function after major resections of the sacrum with bilateral or unilateral sacrifice of sacral nerves.", "content": "The sexual function of nine patients with severance of sacral nerves bilaterally (five patients) or unilaterally (four patients), performed during operations for radical extirpation of tumors of the sacrum or its vicinity, was studied. Sexual histories were obtained and sensibility tests were performed. In four of the five male patients an attempt was made to collect electromyographic recordings from the external urethral and anal sphincters during ejaculation. Bilateral loss of S3 to S5 nerves in two women seemed not to affect their sexual function. Bilateral loss of S2 to S5 nerves in one man was compatible with gratifying sexual intercourse, the stimulation for erection being purely psychogenic, and \"ejaculation\" of a dripping nature. Unilateral loss of all sacral nerves did not impair previously normal sexual function, although the penises and vulvae of these patients were anesthetic on one side. The sensibility of the penis seemed to be subserved by the second sacral nerve. The myoelectric activity of the striated urethral and anal sphincters during ejaculation recorded in one patient with unilateral total loss of sacral nerves was normal bilaterally considering the duration of, and intervals between, the clonic contractions.", "contents": "Sexual function after major resections of the sacrum with bilateral or unilateral sacrifice of sacral nerves. The sexual function of nine patients with severance of sacral nerves bilaterally (five patients) or unilaterally (four patients), performed during operations for radical extirpation of tumors of the sacrum or its vicinity, was studied. Sexual histories were obtained and sensibility tests were performed. In four of the five male patients an attempt was made to collect electromyographic recordings from the external urethral and anal sphincters during ejaculation. Bilateral loss of S3 to S5 nerves in two women seemed not to affect their sexual function. Bilateral loss of S2 to S5 nerves in one man was compatible with gratifying sexual intercourse, the stimulation for erection being purely psychogenic, and \"ejaculation\" of a dripping nature. Unilateral loss of all sacral nerves did not impair previously normal sexual function, although the penises and vulvae of these patients were anesthetic on one side. The sensibility of the penis seemed to be subserved by the second sacral nerve. The myoelectric activity of the striated urethral and anal sphincters during ejaculation recorded in one patient with unilateral total loss of sacral nerves was normal bilaterally considering the duration of, and intervals between, the clonic contractions.", "PMID": 971772}
{"id": "pubmed23n0032_8688", "title": "Prolactin after gonadotropin-induced pregnancy.", "content": "Prolactin levels during a gonadotropin-induced pregnancy have not been previously reported. A patient with Forbes-Albright syndrome is described. She received radiation therapy, with cessation of her galactorrhea, but she remained amenorrheic. Three years after irradiation, a pregnancy was successful induced with human menopausal gonadotropins and human chorionic gonadotropin. Prolactin levels determined prior to gonadotropin therapy, during an insulin hypoglycemia stimulation test, serially during pregnancy, and postpartum during lactation are presented. These levels are compared with the previously reported levels for basal prolactin, response to insulin hypoglycemia, pregnancy, and lactation. Possible etiologies for the abnormal values and responses obtained from investigation of this patient are discussed.", "contents": "Prolactin after gonadotropin-induced pregnancy. Prolactin levels during a gonadotropin-induced pregnancy have not been previously reported. A patient with Forbes-Albright syndrome is described. She received radiation therapy, with cessation of her galactorrhea, but she remained amenorrheic. Three years after irradiation, a pregnancy was successful induced with human menopausal gonadotropins and human chorionic gonadotropin. Prolactin levels determined prior to gonadotropin therapy, during an insulin hypoglycemia stimulation test, serially during pregnancy, and postpartum during lactation are presented. These levels are compared with the previously reported levels for basal prolactin, response to insulin hypoglycemia, pregnancy, and lactation. Possible etiologies for the abnormal values and responses obtained from investigation of this patient are discussed.", "PMID": 971773}
{"id": "pubmed23n0032_8689", "title": "Plasma prostaglandin F 2-alpha levels in dysmenorrehic women.", "content": "Plasma prostaglandin F 2-alpha (PGF 2-alpha) concentrations were compared in nine ovulatory dysmenorrheic women, one dysmenorrheic oral contraceptive user, and two nondysmenorrheic control subjects, in an effort to demonstrate a relationship between plasma PGF 2-alpha levels and dysmenorrhea. In addition, the effects of aspirin, a known inhibitorof prostaglandins synthesis, on dysmenorrhea and on PGF 2-alpha levels were investigated. No statistical difference was demonstrated between the plasma PGF 2-alpha levels of dysmenorrheic and nondysmenorrheic subjects throughout the menstrual cycle. Attainment of an adequate salicylate level was accompanied by a significant decrease in PGF 2-alpha levels. All dysmenorrheic subjects reported improvement in symptoms while taking aspirin. The greatest subjective relief was reported by women who began taking aspirin (10 grains every 4 hours) 3 or more days prior to the onset of bleeding.", "contents": "Plasma prostaglandin F 2-alpha levels in dysmenorrehic women. Plasma prostaglandin F 2-alpha (PGF 2-alpha) concentrations were compared in nine ovulatory dysmenorrheic women, one dysmenorrheic oral contraceptive user, and two nondysmenorrheic control subjects, in an effort to demonstrate a relationship between plasma PGF 2-alpha levels and dysmenorrhea. In addition, the effects of aspirin, a known inhibitorof prostaglandins synthesis, on dysmenorrhea and on PGF 2-alpha levels were investigated. No statistical difference was demonstrated between the plasma PGF 2-alpha levels of dysmenorrheic and nondysmenorrheic subjects throughout the menstrual cycle. Attainment of an adequate salicylate level was accompanied by a significant decrease in PGF 2-alpha levels. All dysmenorrheic subjects reported improvement in symptoms while taking aspirin. The greatest subjective relief was reported by women who began taking aspirin (10 grains every 4 hours) 3 or more days prior to the onset of bleeding.", "PMID": 971774}
{"id": "pubmed23n0032_8690", "title": "Antispermatozoal effects of human seminal plasma--an immunologic phenomenon.", "content": "Semen samples were obtained from seven men who had self-agglutination of spermatozoa, and a spermatozoal antibody in the immunoglobulin G (IgG) fraction of their blood. The seminal plasma of these men produced spermatozoal immobilization and agglutination when added to normal semen from healthy men. Analysis of the seminal plasma samples showed that they contained IgA and complements C-3 and C-4, whereas only 1 of 10 samples from normal men contained IgA, and none had complements C-3 and C-4. The IgG levels of the seminal plasma from the study group were higher than those of the controls. It is suggested that the mechanism of spermagglutination is one of autooimmunization in which the main antibody is IgA from the genital tract, augmented by a circulating antibody from the IgG fraction of the blood.", "contents": "Antispermatozoal effects of human seminal plasma--an immunologic phenomenon. Semen samples were obtained from seven men who had self-agglutination of spermatozoa, and a spermatozoal antibody in the immunoglobulin G (IgG) fraction of their blood. The seminal plasma of these men produced spermatozoal immobilization and agglutination when added to normal semen from healthy men. Analysis of the seminal plasma samples showed that they contained IgA and complements C-3 and C-4, whereas only 1 of 10 samples from normal men contained IgA, and none had complements C-3 and C-4. The IgG levels of the seminal plasma from the study group were higher than those of the controls. It is suggested that the mechanism of spermagglutination is one of autooimmunization in which the main antibody is IgA from the genital tract, augmented by a circulating antibody from the IgG fraction of the blood.", "PMID": 971775}
{"id": "pubmed23n0032_8691", "title": "Clinical experience with sperm antibody testing.", "content": "Of the 724 patients examined for circulating spermagglutinating antibodies by the Kibrick spermagglutination test, 95 (13.1%) were found to be positive. Almost 20% of the 406 male patients demonstrated autoantibodies. This high incidence may be explained by the selected population studied. Of 50 males tested who demonstrated more than 10% agglutination in their semen, but otherwise were normospermic, 42% demonstrated autoantibodies. Of the 318 females examined, 14 (4.4%) demonstrated sperm-agglutinating antibodies. Of the 315 male and female patients examined for circulating sperm-immobilizing antibodies by the Isojima sperm-immobilization test, 14 (4.5%) were found to have antibodies. Of the 162 males treated, 10 (6%) were positive for antibodies by this technique, as were 4 (3%) of the 153 women tested.", "contents": "Clinical experience with sperm antibody testing. Of the 724 patients examined for circulating spermagglutinating antibodies by the Kibrick spermagglutination test, 95 (13.1%) were found to be positive. Almost 20% of the 406 male patients demonstrated autoantibodies. This high incidence may be explained by the selected population studied. Of 50 males tested who demonstrated more than 10% agglutination in their semen, but otherwise were normospermic, 42% demonstrated autoantibodies. Of the 318 females examined, 14 (4.4%) demonstrated sperm-agglutinating antibodies. Of the 315 male and female patients examined for circulating sperm-immobilizing antibodies by the Isojima sperm-immobilization test, 14 (4.5%) were found to have antibodies. Of the 162 males treated, 10 (6%) were positive for antibodies by this technique, as were 4 (3%) of the 153 women tested.", "PMID": 971776}
{"id": "pubmed23n0032_8692", "title": "Wound healing: a model for the study of diabetic angiopathy.", "content": "Wound healing as a model for diabetic angiopathy has been studied by light and electron microscopy. Biochemical studies of the rate of incorporation of 3H-proline and 3H-thymidine into collagen and DNA, respectively, have confirmed the morphologic observations. In both the normal and the diabetic, there was a marked decrease in the rate of collagen and DNA synthesis, suggesting that most of the cells in the biopsies were stunned by the injury and ceased DNA replication during the initial phase. In control mice this decrease was followed by a modest but significant burst of DNA replication, which peaked at two hours and by the fourth hour had returned to the one-hour level. In the diabetic this burst of DNA replication was absent and no capillary morphogenesis was seen at two, four, and eight hours. At 16 hours, there were only a few abnormal nascent vessels observed in the diabetic and antiserum-treated mice. The peak in the rate of collagen synthesis at four hours correlated well with the condensation of collagen at the wound margin and the fibroblast rough-endoplasmic-reticulum (RER) proliferation. In the diabetic mice, there was a significantly attenuated rate of collagen synthesis for the entire 16-hour period. The lack of DNA replication, capillary morphogenesis, fibroblast RER proliferation, and decreased collagen synthesis in the diabetic mouse can be considered interrelated and significant factors in the diabetic's impaired response to cellular injury. In view of the increased frequency and severity of injury to the circulation of the diabetic and the impaired response to repair such injury, it is likely that wound healing is a promising model for diabetic angiopathy.", "contents": "Wound healing: a model for the study of diabetic angiopathy. Wound healing as a model for diabetic angiopathy has been studied by light and electron microscopy. Biochemical studies of the rate of incorporation of 3H-proline and 3H-thymidine into collagen and DNA, respectively, have confirmed the morphologic observations. In both the normal and the diabetic, there was a marked decrease in the rate of collagen and DNA synthesis, suggesting that most of the cells in the biopsies were stunned by the injury and ceased DNA replication during the initial phase. In control mice this decrease was followed by a modest but significant burst of DNA replication, which peaked at two hours and by the fourth hour had returned to the one-hour level. In the diabetic this burst of DNA replication was absent and no capillary morphogenesis was seen at two, four, and eight hours. At 16 hours, there were only a few abnormal nascent vessels observed in the diabetic and antiserum-treated mice. The peak in the rate of collagen synthesis at four hours correlated well with the condensation of collagen at the wound margin and the fibroblast rough-endoplasmic-reticulum (RER) proliferation. In the diabetic mice, there was a significantly attenuated rate of collagen synthesis for the entire 16-hour period. The lack of DNA replication, capillary morphogenesis, fibroblast RER proliferation, and decreased collagen synthesis in the diabetic mouse can be considered interrelated and significant factors in the diabetic's impaired response to cellular injury. In view of the increased frequency and severity of injury to the circulation of the diabetic and the impaired response to repair such injury, it is likely that wound healing is a promising model for diabetic angiopathy.", "PMID": 971788}
{"id": "pubmed23n0032_8693", "title": "The problems of retinal blood flow in diabetes.", "content": "Three methods are available for measurement of retinal blood flow: cinefluroescein angiography, measurement of mean transit time, and estimation of volume flow by laser-Dopler velocimetry. Cineangiography requires intra-arterial injection for volume flow measurement, and this greatly inhibits its use. Difficulties also arise in estimating the relation of the axial to the mean flow, since it is now known that the retinal flow is plug flow. Transit time measurement using fluroescein has recently been automated and thus allows for more accurate measurement and better estimation of volume flow. The problems of estimating vascular volume and allowing for leakage have not been overcome. Laser-Doppler velocimetry measures mean red cell velocity but its relation to volume flow has not been established. Using the mean-transit-time method, several authors have found reduced transit time and increased volume flow in patients with mild and no retinopathy, compared to normals and those with more severe lesions, This most probably is due to autoregulatory adaptation and, possibly, to hypoxia. In more severe retinopathy, flow is reduced and there are large areas of nonperfusion. These areas are the result of abnormality of the endothelial cells and also of the blood within the vessels, which probably coagulates more easily than in normals. These areas are of importance because they stimulate the growth of new vessels.", "contents": "The problems of retinal blood flow in diabetes. Three methods are available for measurement of retinal blood flow: cinefluroescein angiography, measurement of mean transit time, and estimation of volume flow by laser-Dopler velocimetry. Cineangiography requires intra-arterial injection for volume flow measurement, and this greatly inhibits its use. Difficulties also arise in estimating the relation of the axial to the mean flow, since it is now known that the retinal flow is plug flow. Transit time measurement using fluroescein has recently been automated and thus allows for more accurate measurement and better estimation of volume flow. The problems of estimating vascular volume and allowing for leakage have not been overcome. Laser-Doppler velocimetry measures mean red cell velocity but its relation to volume flow has not been established. Using the mean-transit-time method, several authors have found reduced transit time and increased volume flow in patients with mild and no retinopathy, compared to normals and those with more severe lesions, This most probably is due to autoregulatory adaptation and, possibly, to hypoxia. In more severe retinopathy, flow is reduced and there are large areas of nonperfusion. These areas are the result of abnormality of the endothelial cells and also of the blood within the vessels, which probably coagulates more easily than in normals. These areas are of importance because they stimulate the growth of new vessels.", "PMID": 971789}
{"id": "pubmed23n0032_8694", "title": "Plasma protein changes, blood viscosity, and diabetic microangiopathy.", "content": "Diabetic microangiopathy is a slowly progressive condition that usually manifests itself years after the onset of the carbohydrate-metabolism disturbance. It is a far more striking problem in some diabetics than in others. Differences in the pattern of its effect in several body systems suggest that the local vascular disturbance is a mixture of a general microcirculatory change in diabetes and a specific vascular alteration in each tissue. Its manifestations in the individual diabetic appear to be influenced by both elements. Evidence is presented that plasma protein changes in diabetes and their effects on blood flow play a role in acclerating the rate of progression of diabetic microangiopathy by raising plasma viscosity and by increasing the affinity of erythrocytes for each other. The plasma protein change is dominated by a possibly hormonally mediated pattern of alpha-globulin elevation seen in many chronic disorders. This elevation of acute-phase proteins is not likely by itself to produce diabetic microangiopathy, but it may cause an additional stress on the metabolically disturbed diabetic microcirculation. The basic change is a disturbance in the average molecular shape of the plasma proteins that both directly increases plasma viscosity and enhances the aggregation of erythrocytes. Studies are now in progress to determine which of these two mechanisms is more likely to be important in accelerating the development of diabetic microangiopathy.", "contents": "Plasma protein changes, blood viscosity, and diabetic microangiopathy. Diabetic microangiopathy is a slowly progressive condition that usually manifests itself years after the onset of the carbohydrate-metabolism disturbance. It is a far more striking problem in some diabetics than in others. Differences in the pattern of its effect in several body systems suggest that the local vascular disturbance is a mixture of a general microcirculatory change in diabetes and a specific vascular alteration in each tissue. Its manifestations in the individual diabetic appear to be influenced by both elements. Evidence is presented that plasma protein changes in diabetes and their effects on blood flow play a role in acclerating the rate of progression of diabetic microangiopathy by raising plasma viscosity and by increasing the affinity of erythrocytes for each other. The plasma protein change is dominated by a possibly hormonally mediated pattern of alpha-globulin elevation seen in many chronic disorders. This elevation of acute-phase proteins is not likely by itself to produce diabetic microangiopathy, but it may cause an additional stress on the metabolically disturbed diabetic microcirculation. The basic change is a disturbance in the average molecular shape of the plasma proteins that both directly increases plasma viscosity and enhances the aggregation of erythrocytes. Studies are now in progress to determine which of these two mechanisms is more likely to be important in accelerating the development of diabetic microangiopathy.", "PMID": 971790}
{"id": "pubmed23n0032_8695", "title": "Renal function changes in diabetes.", "content": "The present studies show that a number of functional abnormalities are present in the kidney in early diabetes, especially during poor regulation. Normalization of these functions and changes in renal and glomerular size are found during complete diabetes control. The results further support the idea that great efforts should be made to maintain the best possible control situation in diabetic patients. Our studies also indicate that antihypertensive therapy may be beneficial for young, proteinuric diabetics and may postpone the stage of renal insufficiency.", "contents": "Renal function changes in diabetes. The present studies show that a number of functional abnormalities are present in the kidney in early diabetes, especially during poor regulation. Normalization of these functions and changes in renal and glomerular size are found during complete diabetes control. The results further support the idea that great efforts should be made to maintain the best possible control situation in diabetic patients. Our studies also indicate that antihypertensive therapy may be beneficial for young, proteinuric diabetics and may postpone the stage of renal insufficiency.", "PMID": 971791}
{"id": "pubmed23n0032_8696", "title": "Leukocyte chemotaxis in diabetic patients and their nondiabetic first-degree relatives.", "content": "Leukocyte chemotaxis wasmeasured in 108 subjects: 20 normal controls, 36 insulin-taking diabetics (15 being index patients), and 52 nondiabetic first-degree relatives of index patients. The relatives had a mean chemotactic index (CI) significantly lower than that of the controls (individually, 25 per cent had CIs lower than the lowest of the controls); and the diabetics had a mean CI significantly lower than that of the relatives. The impairment of leukocyte chemotaxis measured in the first-degree relatives of diabetic index patients does not appear to be associated with an increased susceptibility to infection and suggests that the impaired leukotaxis is due to an inherent defect--perhaps genetic--in leukocyte function.", "contents": "Leukocyte chemotaxis in diabetic patients and their nondiabetic first-degree relatives. Leukocyte chemotaxis wasmeasured in 108 subjects: 20 normal controls, 36 insulin-taking diabetics (15 being index patients), and 52 nondiabetic first-degree relatives of index patients. The relatives had a mean chemotactic index (CI) significantly lower than that of the controls (individually, 25 per cent had CIs lower than the lowest of the controls); and the diabetics had a mean CI significantly lower than that of the relatives. The impairment of leukocyte chemotaxis measured in the first-degree relatives of diabetic index patients does not appear to be associated with an increased susceptibility to infection and suggests that the impaired leukotaxis is due to an inherent defect--perhaps genetic--in leukocyte function.", "PMID": 971792}
{"id": "pubmed23n0032_8697", "title": "Red-cell aggregation and red-cell deformability in diabetes.", "content": "The anomaly of the viscosity of human blood is more pronounced in diabetics. This is caused by an increase in plasma viscosity, a more pronounced red-cell aggregation, and a reduction of individual cell deformability. The changes in viscosity and in red-cell aggregation both are the consequence of abnormal plasma proteins, the incidence of which is largely independent of the onset and duration of disease, and actual metabolic state. The presence of complicating infectious diseases further aggravates the pathologic red-cell aggregation. The decreased red-cell deformability is largely independent on onset, duration, and complications but depends critically in the incident metabolic control of the diabetics. The possible role of hemorrheologic factors in the development of microangiopathy is discussed.", "contents": "Red-cell aggregation and red-cell deformability in diabetes. The anomaly of the viscosity of human blood is more pronounced in diabetics. This is caused by an increase in plasma viscosity, a more pronounced red-cell aggregation, and a reduction of individual cell deformability. The changes in viscosity and in red-cell aggregation both are the consequence of abnormal plasma proteins, the incidence of which is largely independent of the onset and duration of disease, and actual metabolic state. The presence of complicating infectious diseases further aggravates the pathologic red-cell aggregation. The decreased red-cell deformability is largely independent on onset, duration, and complications but depends critically in the incident metabolic control of the diabetics. The possible role of hemorrheologic factors in the development of microangiopathy is discussed.", "PMID": 971793}
{"id": "pubmed23n0032_8698", "title": "Mean retinal circulation time as determined by fluorescein angiography in normal, prediabetic, and chemical-diabetic subjects.", "content": "A preliminary survey has been completed using manual densitometric technics to determine the mean retinal circulation times in groups of normal controls, offspring to two diabetic parents with normal glucose tolerance (prediabetics), and offspring of two diabetic parents with abnormal glucose tolerance (chemical diabetics). Comparisons of the mean retinal circulation time showed differences between the left eye and right eye in prediabetic and chemical diabetic groups and a sex difference in both normals and prediabetics. In addition, both age and per cent ideal body weight were inversely related to the mean retinal circulation time. The levels of fasting serum cholesterol, triglyceride, and growth hormone, in many instances, also appeared to be inversely related to the mean retinal circulation time. Similarly, the degree of glucose tolerance (determined by the area under the glucose curve above baseline) was significantly inversely related to the mean retinal circulation time. The mean retinal circulation time adjusted for per cent ideal weight was analyzed separately for both right eye and left eye, and a significantly shorter mean retinal circulation time was noted, particularly in males, for prediabetics than for normal controls and for chemical diabetics than for both prediabetics and normals. Analysis of the mean retinal circulation time adjusted for age showed similar differences. It is postulated that the genetic prediabetic state with or without glucose intolerance might be associated with significant alterations of mean retinal circulation time independent of age and per cent ideal weight. It is also suggested that a number of potentially meaningful interrelationships between the degree of glucose intolerance and/or hyperlipidemia might exist and should be further quantified.", "contents": "Mean retinal circulation time as determined by fluorescein angiography in normal, prediabetic, and chemical-diabetic subjects. A preliminary survey has been completed using manual densitometric technics to determine the mean retinal circulation times in groups of normal controls, offspring to two diabetic parents with normal glucose tolerance (prediabetics), and offspring of two diabetic parents with abnormal glucose tolerance (chemical diabetics). Comparisons of the mean retinal circulation time showed differences between the left eye and right eye in prediabetic and chemical diabetic groups and a sex difference in both normals and prediabetics. In addition, both age and per cent ideal body weight were inversely related to the mean retinal circulation time. The levels of fasting serum cholesterol, triglyceride, and growth hormone, in many instances, also appeared to be inversely related to the mean retinal circulation time. Similarly, the degree of glucose tolerance (determined by the area under the glucose curve above baseline) was significantly inversely related to the mean retinal circulation time. The mean retinal circulation time adjusted for per cent ideal weight was analyzed separately for both right eye and left eye, and a significantly shorter mean retinal circulation time was noted, particularly in males, for prediabetics than for normal controls and for chemical diabetics than for both prediabetics and normals. Analysis of the mean retinal circulation time adjusted for age showed similar differences. It is postulated that the genetic prediabetic state with or without glucose intolerance might be associated with significant alterations of mean retinal circulation time independent of age and per cent ideal weight. It is also suggested that a number of potentially meaningful interrelationships between the degree of glucose intolerance and/or hyperlipidemia might exist and should be further quantified.", "PMID": 971794}
{"id": "pubmed23n0032_8699", "title": "Biochemical alterations of the human glomerular basement membrane in diabetes.", "content": "Only from the kidney glomerulus can the capillary basement membrane be isolated in quantities sufficient for biochemical analyses. In this study an attempt has been made to obtain a basement membrane as pure as possible, without contamination from cells or mesangium. In diabetic nephropathy, the glomerular basement membrane shows a decreased content of cystine, but hydroxylysine concentrations was only slightly higher than in normal material. The concentration of glucose was significantly increased. Immunofluorescent studies indicated the presence of serum proteins in the diabetic glomerular basement membrane, probably as a result of increased membrane permeability.", "contents": "Biochemical alterations of the human glomerular basement membrane in diabetes. Only from the kidney glomerulus can the capillary basement membrane be isolated in quantities sufficient for biochemical analyses. In this study an attempt has been made to obtain a basement membrane as pure as possible, without contamination from cells or mesangium. In diabetic nephropathy, the glomerular basement membrane shows a decreased content of cystine, but hydroxylysine concentrations was only slightly higher than in normal material. The concentration of glucose was significantly increased. Immunofluorescent studies indicated the presence of serum proteins in the diabetic glomerular basement membrane, probably as a result of increased membrane permeability.", "PMID": 971795}
{"id": "pubmed23n0032_8700", "title": "The relationship between diabetic capillaropathy and myocardial infarction: a hypothesis.", "content": "It is believed that diabetic patients with clinical evidence of diabetic capillaropathy have a greater risk of myocardial infarction than those with the same duration of disease but no evidence of capillaropathy. If the hypothesis presented here is correct, then early attention to diet, perhaps as a means of slowing the rate of atherosclerosis, will be as important as insulin in reducing current mortality statistics.", "contents": "The relationship between diabetic capillaropathy and myocardial infarction: a hypothesis. It is believed that diabetic patients with clinical evidence of diabetic capillaropathy have a greater risk of myocardial infarction than those with the same duration of disease but no evidence of capillaropathy. If the hypothesis presented here is correct, then early attention to diet, perhaps as a means of slowing the rate of atherosclerosis, will be as important as insulin in reducing current mortality statistics.", "PMID": 971796}
{"id": "pubmed23n0032_8701", "title": "RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence.", "content": "Introduction of RP4 plasmid into Agrobacterium tumefaciens promotes the transfer on solid medium of large virulence-associated plasmids from virulent donor strains to a plasmidless avirulent recipient. Exconjugants were selected for the ability to utilize octopine or nopaline as the sole source of arginine, traits which are coded for by virulence-associated plasmids in the strains employed here. All exconjugants retained the arginine auxotrophy of the recipient strain, and were resistant to ampicillin and kanamycin, drugs to which RP4 confers resistance. Five exconjugant clones from one cross were shown by alkaline sucrose gradient analysis to contain both RP4 plasmid and the large virulence-associated plasmid of the donor strain. All five exconjugants exhibited virulence on carrot, sunflower and kalanchoe plants. These results indicate that virulence and the ability to degrade octopine are plasmid-borne traits in A. tumefaciens strains 15955 and A6, and extend the evidence that large plasmids in A. tumefaciens are vectors of virulence genes.", "contents": "RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence. Introduction of RP4 plasmid into Agrobacterium tumefaciens promotes the transfer on solid medium of large virulence-associated plasmids from virulent donor strains to a plasmidless avirulent recipient. Exconjugants were selected for the ability to utilize octopine or nopaline as the sole source of arginine, traits which are coded for by virulence-associated plasmids in the strains employed here. All exconjugants retained the arginine auxotrophy of the recipient strain, and were resistant to ampicillin and kanamycin, drugs to which RP4 confers resistance. Five exconjugant clones from one cross were shown by alkaline sucrose gradient analysis to contain both RP4 plasmid and the large virulence-associated plasmid of the donor strain. All five exconjugants exhibited virulence on carrot, sunflower and kalanchoe plants. These results indicate that virulence and the ability to degrade octopine are plasmid-borne traits in A. tumefaciens strains 15955 and A6, and extend the evidence that large plasmids in A. tumefaciens are vectors of virulence genes.", "PMID": 971805}
{"id": "pubmed23n0032_8702", "title": "Diploid gynogenesis in the Mexican axolotl.", "content": "Gynogenetic diploid axolotls were produced by activating eggs with ultraviolet-inactivated sperm, and then subjecting the activated eggs to heat shock. Optimal conditions for ultraviolet inactivation of the sperm, and for suppression of the second meiotic division by heat shock, were established. Gynogenetic diploids produced by these procedures included progeny homozygous for recessive alleles carried by a heterozygous mother. Gynogenesis could, therefore, be used to uncover new mutations more rapidly than by conventional inbreeding techniques. However, some difficulty was encountered in recognizing mutant phenotypes because of the high incidence of abnormalities and deaths. Defective embryos probably resulted from a combination of heat-shock-induced damage to the eggs and the expression of deleterious recessive alleles carried by the mother.", "contents": "Diploid gynogenesis in the Mexican axolotl. Gynogenetic diploid axolotls were produced by activating eggs with ultraviolet-inactivated sperm, and then subjecting the activated eggs to heat shock. Optimal conditions for ultraviolet inactivation of the sperm, and for suppression of the second meiotic division by heat shock, were established. Gynogenetic diploids produced by these procedures included progeny homozygous for recessive alleles carried by a heterozygous mother. Gynogenesis could, therefore, be used to uncover new mutations more rapidly than by conventional inbreeding techniques. However, some difficulty was encountered in recognizing mutant phenotypes because of the high incidence of abnormalities and deaths. Defective embryos probably resulted from a combination of heat-shock-induced damage to the eggs and the expression of deleterious recessive alleles carried by the mother.", "PMID": 971806}
{"id": "pubmed23n0032_8703", "title": "Evolution of Hawaiian drosophilidae. II. Patterns and rates of chromosome evolution in an antopocerus phylogeny.", "content": "The phylogenetic relationships of seven species of the genus Antopocerus (Family Drosophilidae) have been determined by means of a study of the metaphase configurations and polytene chromosomes. Based on biogeographical, behavioral and cytogenetic information A. longiseta from Molokai is tentatively identified as the primitive species of the genus. The metaphase karyotypes of all Antopocerus species are either five pairs of rod chromosomes and a pair of dots (5R1D), or six rods (6R). Heterochromatin additions converted the dots to rods. Chromosome breakpoints for inversions also are clustered at heterochromatic loci. The chromosome segments between heterochromatic loci may represent sets of functionally related loci, evolving as a unit. The rate of chromosomal inversion substitution is estimated in the origin of the taxon (probably a subgenus of Drosophila rather than a separate genus). It averages no greater than one substitution per 1,000 years, or one per 5,000 generations. The average genetic death rate per generation of one individual per hundred is required to achieve this substitution rate. The rate of inversion substitution during radiation of this taxon may be only 4.4 X 10(-3) times as fast as that present in forming the taxon. Alternatively, radiation may have required only 250,000 years if rates of substitution are the same as in the origination of the taxon. Average rates of substitution reflect genetic accidents, selection pressures and rates of adaptation to new niches, as well as the rate of encountering new niches. Rate of adaptation probably is much greater in this instance than rate of encountering new niches. Rate of adaptation probably is much greater in this instance than rate of encountering new niches. Therefore, the average rate of evolution reflects more nearly biogeographic and ecological factors than genetic factors.", "contents": "Evolution of Hawaiian drosophilidae. II. Patterns and rates of chromosome evolution in an antopocerus phylogeny. The phylogenetic relationships of seven species of the genus Antopocerus (Family Drosophilidae) have been determined by means of a study of the metaphase configurations and polytene chromosomes. Based on biogeographical, behavioral and cytogenetic information A. longiseta from Molokai is tentatively identified as the primitive species of the genus. The metaphase karyotypes of all Antopocerus species are either five pairs of rod chromosomes and a pair of dots (5R1D), or six rods (6R). Heterochromatin additions converted the dots to rods. Chromosome breakpoints for inversions also are clustered at heterochromatic loci. The chromosome segments between heterochromatic loci may represent sets of functionally related loci, evolving as a unit. The rate of chromosomal inversion substitution is estimated in the origin of the taxon (probably a subgenus of Drosophila rather than a separate genus). It averages no greater than one substitution per 1,000 years, or one per 5,000 generations. The average genetic death rate per generation of one individual per hundred is required to achieve this substitution rate. The rate of inversion substitution during radiation of this taxon may be only 4.4 X 10(-3) times as fast as that present in forming the taxon. Alternatively, radiation may have required only 250,000 years if rates of substitution are the same as in the origination of the taxon. Average rates of substitution reflect genetic accidents, selection pressures and rates of adaptation to new niches, as well as the rate of encountering new niches. Rate of adaptation probably is much greater in this instance than rate of encountering new niches. Rate of adaptation probably is much greater in this instance than rate of encountering new niches. Therefore, the average rate of evolution reflects more nearly biogeographic and ecological factors than genetic factors.", "PMID": 971807}
{"id": "pubmed23n0032_8704", "title": "The evolutionary advantage of recombination. II. Individual selection for recombination.", "content": "Based on the FISHER-MULLER theory of the evolution of recombination, an argument can be constructed predicting that a recessive allele favoring recombination will be favored, if there are either favorable or deleterious mutants occurring at other loci. In this case there is no clear distinction between individual and group selection. Computer simulation of populations segregating for recessive or dominant recombination alleles showed selection favoring recombination, except in the case of a dominant recombination allele with deleterious background mutants. The relationship of this work to parallel investigations by WILLIAMS and by STROBECK, MAYNARD SMITH, and CHARLESWORTH is explored. All seem to rely on the same phenomenon. There seems no reason to assume that the evolution of recombination must have occurred by group selection.", "contents": "The evolutionary advantage of recombination. II. Individual selection for recombination. Based on the FISHER-MULLER theory of the evolution of recombination, an argument can be constructed predicting that a recessive allele favoring recombination will be favored, if there are either favorable or deleterious mutants occurring at other loci. In this case there is no clear distinction between individual and group selection. Computer simulation of populations segregating for recessive or dominant recombination alleles showed selection favoring recombination, except in the case of a dominant recombination allele with deleterious background mutants. The relationship of this work to parallel investigations by WILLIAMS and by STROBECK, MAYNARD SMITH, and CHARLESWORTH is explored. All seem to rely on the same phenomenon. There seems no reason to assume that the evolution of recombination must have occurred by group selection.", "PMID": 971808}
{"id": "pubmed23n0032_8705", "title": "Gene conversion: a hitherto overlooked parameter in population genetics.", "content": "Gene conversion causes deviations from the 2:2 segregation of allele pairs in meiosis. Thus, gene conversion is a potential cause for changes of allele frequencies in populations. Equations are derived for the effects of conversion in a large random-mating population. The influence of gene conversion on allele frequencies is compared with that of spontaneous mutation and meiotic drive.", "contents": "Gene conversion: a hitherto overlooked parameter in population genetics. Gene conversion causes deviations from the 2:2 segregation of allele pairs in meiosis. Thus, gene conversion is a potential cause for changes of allele frequencies in populations. Equations are derived for the effects of conversion in a large random-mating population. The influence of gene conversion on allele frequencies is compared with that of spontaneous mutation and meiotic drive.", "PMID": 971809}
{"id": "pubmed23n0032_8706", "title": "Clines with variable migration.", "content": "The consequences of a discontinuity in the migration rate and of a geographical barrier in the habitat are studied in a diffusion model of migration and selection. The treatment is restricted to a single diallelic locus in a monoecious population in the absence of mutation and random drift. It is supposed further that migration is independent of genotype, the population density remains constant and uniform, and Hardy-Weinberg proportions obtain locally. It is shown that a discontinuity in the migration rate leads to a jump in the slope of the gene frequency, but not in the gene frequency itself, while a localized geographical barrier has precisely the opposite effect. These features of the gene frequency behavior are quantitatively related to the migration rate. The influence of the above inhomogeneities in migration on the maintenance of an allele in an environmental pocket is examined. The extent to which the critical condition for polymorphism is made less stringent by decreased migration outside the pocket and by a geographical barrier between the pocket and the rest of the habitat is evaluated.", "contents": "Clines with variable migration. The consequences of a discontinuity in the migration rate and of a geographical barrier in the habitat are studied in a diffusion model of migration and selection. The treatment is restricted to a single diallelic locus in a monoecious population in the absence of mutation and random drift. It is supposed further that migration is independent of genotype, the population density remains constant and uniform, and Hardy-Weinberg proportions obtain locally. It is shown that a discontinuity in the migration rate leads to a jump in the slope of the gene frequency, but not in the gene frequency itself, while a localized geographical barrier has precisely the opposite effect. These features of the gene frequency behavior are quantitatively related to the migration rate. The influence of the above inhomogeneities in migration on the maintenance of an allele in an environmental pocket is examined. The extent to which the critical condition for polymorphism is made less stringent by decreased migration outside the pocket and by a geographical barrier between the pocket and the rest of the habitat is evaluated.", "PMID": 971810}
{"id": "pubmed23n0032_8707", "title": "Genetic variation in heterogeneous environments.", "content": "A model of population structure in heterogeneous environments is described and conditions sufficient for maintaining a polymorphism are derived. The absolute fitness of any genotype is regarded as a function of location in the niche space and the population density at that location. Two modes of habitat selection are examined: (1) organisms are distributed uniformly over the environment; and (2) each organism selects to occupy that habitat in which it is most fit (\"optimal habitant selection\"). Sufficient conditions for maintenance of genetic polymorphisms are derived for both models. In populations which do not practice habitat selection heterozygote superiority averaged over the environment is sufficient to guarantee the existence of polymorphisms. Comparable conditions for populations which practice optimal habitat selction are much less restrictive. If the heterozygotes are superior to one homozygote in any one part of the niche and to the other homozygote in any other part of the niche then a polymorphism will be defined. A positive correlation between genetic and environmental variation follows from the model with habitat selection, but not from the other. The adaptive significance of polymorphisms thus depends on how animals behave.", "contents": "Genetic variation in heterogeneous environments. A model of population structure in heterogeneous environments is described and conditions sufficient for maintaining a polymorphism are derived. The absolute fitness of any genotype is regarded as a function of location in the niche space and the population density at that location. Two modes of habitat selection are examined: (1) organisms are distributed uniformly over the environment; and (2) each organism selects to occupy that habitat in which it is most fit (\"optimal habitant selection\"). Sufficient conditions for maintenance of genetic polymorphisms are derived for both models. In populations which do not practice habitat selection heterozygote superiority averaged over the environment is sufficient to guarantee the existence of polymorphisms. Comparable conditions for populations which practice optimal habitat selction are much less restrictive. If the heterozygotes are superior to one homozygote in any one part of the niche and to the other homozygote in any other part of the niche then a polymorphism will be defined. A positive correlation between genetic and environmental variation follows from the model with habitat selection, but not from the other. The adaptive significance of polymorphisms thus depends on how animals behave.", "PMID": 971811}
{"id": "pubmed23n0032_8708", "title": "[Observation of a distinct humeral spur formation].", "content": "A 23 year old male with a mirror right hand, duplicated ulna and aplasia of the radius is presented. A large deformity of the distal and anterior surface of the humerus was also noted. Based on clinical documentation and X-rays it was felt that this unusual bony formation probably represented a minimal rudiment of the radius and that the bone deformity at the distal end of the humerus did not represent a supracondylar humeral spur which is occasionally seen in humerus without any concommittent congenital deformities, as is nearly always seen in mammals.", "contents": "[Observation of a distinct humeral spur formation]. A 23 year old male with a mirror right hand, duplicated ulna and aplasia of the radius is presented. A large deformity of the distal and anterior surface of the humerus was also noted. Based on clinical documentation and X-rays it was felt that this unusual bony formation probably represented a minimal rudiment of the radius and that the bone deformity at the distal end of the humerus did not represent a supracondylar humeral spur which is occasionally seen in humerus without any concommittent congenital deformities, as is nearly always seen in mammals.", "PMID": 971866}
{"id": "pubmed23n0032_8709", "title": "[Static and dynamic studies on finger arthrodeses].", "content": "Some general techniques in arthrodesis of finger joints are described. Then biomechanical measurements were made after compression arthrodesis with screws, crossed KIRSCHNER wires, tension band wiring, compression plates (Swiss AO), and self compression plates, creascent shaped (MITTELMEIER). By screws, a maximal compression between 25 and 34 kp is achieved. Using a selfcompressing, bent, crescent shaped plate, one gets a compression of about 18 kp. This osteosynthesis resists a high deflection momentum if edgewise inserted.", "contents": "[Static and dynamic studies on finger arthrodeses]. Some general techniques in arthrodesis of finger joints are described. Then biomechanical measurements were made after compression arthrodesis with screws, crossed KIRSCHNER wires, tension band wiring, compression plates (Swiss AO), and self compression plates, creascent shaped (MITTELMEIER). By screws, a maximal compression between 25 and 34 kp is achieved. Using a selfcompressing, bent, crescent shaped plate, one gets a compression of about 18 kp. This osteosynthesis resists a high deflection momentum if edgewise inserted.", "PMID": 971867}
{"id": "pubmed23n0032_8710", "title": "[Advances in osteosynthesis of hand fractures].", "content": "In recent years stable internal fixation of hand fractures has become more commonly used though the majority of fractures of the hand bones are still treated successfully by other orthopedic means. We feel that in selected fractures internal fixation achieves better results than conservative treatment. Operative methods with less traumatic skin incisions for the fixation of multiple metacarpal fractures are proposed. A retrograde screw fixation technique is presented which permitts application of compression in very small bone fragments, which risk to be crashed by standard screwing. Results of 60 internal fixations of carpometacarpal fractures of the first metacarpal are reported. Follow-up examination of 41 patients between 1-7 years following surgery, revealed posttraumatic arthritis only in 3 joints, although remaining small articular irregularities were frequently seen on X-ray. Secondary angulation occured in four cases in spite fixation. This was due to poor cortical fixation on the palmar side of the fracture, thus in such cases temporary plaster cast should entirely be applied.", "contents": "[Advances in osteosynthesis of hand fractures]. In recent years stable internal fixation of hand fractures has become more commonly used though the majority of fractures of the hand bones are still treated successfully by other orthopedic means. We feel that in selected fractures internal fixation achieves better results than conservative treatment. Operative methods with less traumatic skin incisions for the fixation of multiple metacarpal fractures are proposed. A retrograde screw fixation technique is presented which permitts application of compression in very small bone fragments, which risk to be crashed by standard screwing. Results of 60 internal fixations of carpometacarpal fractures of the first metacarpal are reported. Follow-up examination of 41 patients between 1-7 years following surgery, revealed posttraumatic arthritis only in 3 joints, although remaining small articular irregularities were frequently seen on X-ray. Secondary angulation occured in four cases in spite fixation. This was due to poor cortical fixation on the palmar side of the fracture, thus in such cases temporary plaster cast should entirely be applied.", "PMID": 971868}
{"id": "pubmed23n0032_8711", "title": "[Stable osteosynthesis in reconstructive surgery of the hand].", "content": "Stable fixation of the skeleton of the hand hat its merits especially in reconstructive procedures, such as osteotomy for malunited fractures, bone grafting procedures for loss of bone substance, fusion of any of the numerous joints in the hand and finally for finger as well as metacarpal transfer. Stable fixation is useful also in combined procedures on bone, tendons and skin. The aim of stabilization is immediate restauration as a lever arm in the motor system of the extremity. This may be achieved using adequate metalimplants based on biomechanically sound principles as outlined above.", "contents": "[Stable osteosynthesis in reconstructive surgery of the hand]. Stable fixation of the skeleton of the hand hat its merits especially in reconstructive procedures, such as osteotomy for malunited fractures, bone grafting procedures for loss of bone substance, fusion of any of the numerous joints in the hand and finally for finger as well as metacarpal transfer. Stable fixation is useful also in combined procedures on bone, tendons and skin. The aim of stabilization is immediate restauration as a lever arm in the motor system of the extremity. This may be achieved using adequate metalimplants based on biomechanically sound principles as outlined above.", "PMID": 971869}
{"id": "pubmed23n0032_8712", "title": "[Osteosyntheses in rheumatoid arthritis].", "content": "The wrist joint is fused by an original method consisting of a Rush pin from the third metacarpal bone high up in radius and secured against rotation by two staples between carpal bones and radius. The MP-joint of the thumb is arthrodesed by aid of a staple with compression - a special instrumentarium is used. PIP-joints were fused according to BOYES technique and the DIP-joints with compression screws.", "contents": "[Osteosyntheses in rheumatoid arthritis]. The wrist joint is fused by an original method consisting of a Rush pin from the third metacarpal bone high up in radius and secured against rotation by two staples between carpal bones and radius. The MP-joint of the thumb is arthrodesed by aid of a staple with compression - a special instrumentarium is used. PIP-joints were fused according to BOYES technique and the DIP-joints with compression screws.", "PMID": 971870}
{"id": "pubmed23n0032_8713", "title": "[Computer sensomtery as objective examination of sensation in the finger area].", "content": "The authors described what they believe to be a more objective method for evaluation of sensation within the digital pulp. It requires the use of electronic equipment including EEG to record the evoked potentials. With further refinement this method should become a valid tool in the objective examination of sensory patterns.", "contents": "[Computer sensomtery as objective examination of sensation in the finger area]. The authors described what they believe to be a more objective method for evaluation of sensation within the digital pulp. It requires the use of electronic equipment including EEG to record the evoked potentials. With further refinement this method should become a valid tool in the objective examination of sensory patterns.", "PMID": 971871}
{"id": "pubmed23n0032_8714", "title": "[Conservative treatment of extensor tendon injuries].", "content": "Report on 35 cases of mallet finger treated conservatively: a circular plaster cast was modeled in hyperextension of the distal interphalangeal joint. The immobilisation of the whole finger form the tip to the proximal phalanx was maintained for six weeks. The reported results were good or excellent with the exception of two cases.", "contents": "[Conservative treatment of extensor tendon injuries]. Report on 35 cases of mallet finger treated conservatively: a circular plaster cast was modeled in hyperextension of the distal interphalangeal joint. The immobilisation of the whole finger form the tip to the proximal phalanx was maintained for six weeks. The reported results were good or excellent with the exception of two cases.", "PMID": 971872}
{"id": "pubmed23n0032_8715", "title": "[Scintigraphic demonstration of the synovial membrane].", "content": "A new technique to diagnose and control the progress of pcp in the hand is described using Tc 99 m to visualize inflammatory reactions in various tissues. Objectivity, repeatability and the possibility to see the disease in its earliest stages are advantages of the method.", "contents": "[Scintigraphic demonstration of the synovial membrane]. A new technique to diagnose and control the progress of pcp in the hand is described using Tc 99 m to visualize inflammatory reactions in various tissues. Objectivity, repeatability and the possibility to see the disease in its earliest stages are advantages of the method.", "PMID": 971882}
{"id": "pubmed23n0032_8716", "title": "The effect of motor-response-deprivation on contingent negative variation (CNV). III: relation with Maudsley Personality Inventory (MPI).", "content": "The contingent negative variation (CNV) was correlated with Maudsley Personality Inventory (MPI) in 43 healthy adults as follows: (1) The conventional CNV with motor-response showed a negative correlation with the scores of extroversion in the MPI (E-scores). The recovery of the conventional CNV after verbal extinction showed both a negative correlation with the E-scores and a politive correlation with the scores of neuroticism (N-scores). (2) The motor-response-deprived CNV in the stimuli and nonresponse sessions (Sa and Na) showed no correlation with the MPI, because they fluctuated according to the subject's attentiveness. On the other hand, the CNV in the discrimination session (Da) with constant attentiveness to stimuli showed a positive correlation with the N-scores. (3) The response times in the control and response sessions showed a positive correlation with the N-scores in males and a negative correlation in females. The correlation with the E-scores was nearly opposite.", "contents": "The effect of motor-response-deprivation on contingent negative variation (CNV). III: relation with Maudsley Personality Inventory (MPI). The contingent negative variation (CNV) was correlated with Maudsley Personality Inventory (MPI) in 43 healthy adults as follows: (1) The conventional CNV with motor-response showed a negative correlation with the scores of extroversion in the MPI (E-scores). The recovery of the conventional CNV after verbal extinction showed both a negative correlation with the E-scores and a politive correlation with the scores of neuroticism (N-scores). (2) The motor-response-deprived CNV in the stimuli and nonresponse sessions (Sa and Na) showed no correlation with the MPI, because they fluctuated according to the subject's attentiveness. On the other hand, the CNV in the discrimination session (Da) with constant attentiveness to stimuli showed a positive correlation with the N-scores. (3) The response times in the control and response sessions showed a positive correlation with the N-scores in males and a negative correlation in females. The correlation with the E-scores was nearly opposite.", "PMID": 971883}
{"id": "pubmed23n0032_8717", "title": "Effects of L-dopa and vitamin B6 on electroencephalograms of schizophrenic patients: a preliminary report.", "content": "1. To assess the therapeutic effect of low-dose L-Dopa therapy and associated EEG changes in chronic schizophrenia, 10 patients with a mean duration of illness of 12.4 years were treated with L-Dopa for a period of eight weeks during which the dosage was increased progressively from an initial level of 300 mg q.d. biweekly up to 600 mg q.d. The treatment was moderately effective in one case and slightly efficacious in one, produced no significant change in the conditions of seven patients while the remaining patient showed exacerbation; hence a noticeably low rate of improvement. There occurred no significant changes in the EEG pattern in the series of 10 patients on the average. The individual patients' responses, nevertheless, could be classified into three groups: one with no observable EEG changes, the second showing a slight degree of increase in alpha activity and the third exhibiting diminution of alpha activity in the EEG. The patients in the latter two groups all had durations of disease less than 10 years. 2. Observations were made primarily of changes in the EEG in 20 chronically schizophrenic patients with a mean duration of disease of 13 years receiving 60 mg of vitamin B6 (as pyridoxal-5'-phosphate) daily over a period of four weeks. Slight increase of alpha activity and decrease of theta activity in the EEG were noted on the average of the 20 cases, in response to the vitamin B6 therapy. The increase of alpha activity was frequently seen among patients with a duration of illness less than 10 years whose pretreatment EEG pattern had been alpha dominant (five out of 10 cases), whereas a slight ameliorative tendency of EEG was observed only in one out of 10 patients whose pretreatment EEG pattern had been slow-wave dominant. Symptomatic improvement was evident only in one of the 20 cases studied. 3. Observations were made of the therapeutic effect and associated EEG changes in eight patients receiving combined medication of 200 mg L-Dopa and 30 mg vitamin B6 (as pyridoxal-5'-phosphate) daily for a period of 12 weeks. Of these eight patients with a mean duration of disease of 18.3 years, two showed excellent response, three good and three fair; hence good to excellent responses attained in five out of the eight cases or 62.5%. A marked increase in alpha activity in the EEG occurred from the 2nd to 4th weeks onward in all eight cases. The EEG changes were likely to precede the symptomatic improvement. 4. To sum up the results of these three clinical trials, administration of L-Dopa alone resulted in practically no symptomatic improvement or EEG changes in patients with chronic schizophrenia whilst vitamin B6 administered singly as pyridoxal-5'-phosphate scarcely produced significant symptomatic improvement but brought about a slight ameliorative tendency in the EEG of such patients. Both symptomatic amelioration and EEG improvement occurred following combined medication of L-Dopa and vitamin B6...", "contents": "Effects of L-dopa and vitamin B6 on electroencephalograms of schizophrenic patients: a preliminary report. 1. To assess the therapeutic effect of low-dose L-Dopa therapy and associated EEG changes in chronic schizophrenia, 10 patients with a mean duration of illness of 12.4 years were treated with L-Dopa for a period of eight weeks during which the dosage was increased progressively from an initial level of 300 mg q.d. biweekly up to 600 mg q.d. The treatment was moderately effective in one case and slightly efficacious in one, produced no significant change in the conditions of seven patients while the remaining patient showed exacerbation; hence a noticeably low rate of improvement. There occurred no significant changes in the EEG pattern in the series of 10 patients on the average. The individual patients' responses, nevertheless, could be classified into three groups: one with no observable EEG changes, the second showing a slight degree of increase in alpha activity and the third exhibiting diminution of alpha activity in the EEG. The patients in the latter two groups all had durations of disease less than 10 years. 2. Observations were made primarily of changes in the EEG in 20 chronically schizophrenic patients with a mean duration of disease of 13 years receiving 60 mg of vitamin B6 (as pyridoxal-5'-phosphate) daily over a period of four weeks. Slight increase of alpha activity and decrease of theta activity in the EEG were noted on the average of the 20 cases, in response to the vitamin B6 therapy. The increase of alpha activity was frequently seen among patients with a duration of illness less than 10 years whose pretreatment EEG pattern had been alpha dominant (five out of 10 cases), whereas a slight ameliorative tendency of EEG was observed only in one out of 10 patients whose pretreatment EEG pattern had been slow-wave dominant. Symptomatic improvement was evident only in one of the 20 cases studied. 3. Observations were made of the therapeutic effect and associated EEG changes in eight patients receiving combined medication of 200 mg L-Dopa and 30 mg vitamin B6 (as pyridoxal-5'-phosphate) daily for a period of 12 weeks. Of these eight patients with a mean duration of disease of 18.3 years, two showed excellent response, three good and three fair; hence good to excellent responses attained in five out of the eight cases or 62.5%. A marked increase in alpha activity in the EEG occurred from the 2nd to 4th weeks onward in all eight cases. The EEG changes were likely to precede the symptomatic improvement. 4. To sum up the results of these three clinical trials, administration of L-Dopa alone resulted in practically no symptomatic improvement or EEG changes in patients with chronic schizophrenia whilst vitamin B6 administered singly as pyridoxal-5'-phosphate scarcely produced significant symptomatic improvement but brought about a slight ameliorative tendency in the EEG of such patients. Both symptomatic amelioration and EEG improvement occurred following combined medication of L-Dopa and vitamin B6...", "PMID": 971884}
{"id": "pubmed23n0032_8718", "title": "Familial unusual encephalopathy of Binswanger's type without hypertension.", "content": "Clinical and pathological studies have been conducted on two brothers with unusual encephalopathy of Binswanger's type. The disease started in the third decade with steady progressive course leading to death in eight or nine years. The clinical picture was summarized as a combination of organic dementia, extrapyramidal disorders associated with pseudobulbar symptoms and marked pyramidal tract signs. The blood pressure remained always normal during the course. Pathologically, there were diffuse and focal demyelination with sparing of U-fibers, multiple small foci of perivascular softening in the cerebral white matter and in the basal ganglia and severe arteriosclerotic changes of memingeal small arteries and long arteries with 100 to 400 micron caliber in the cerebral white matter. Vessel changes consisted of fibrous intimal proliferation, severe hyalinosis and splitting of intima and/or internal elastic membrane. The histopathological process belonged to the category of subcortical arteriosclerotic encephalopathy of Binswanger's type. There has been some discussion as to differential diagnosis among various forms of vasculitis such as cerebral endangiitis obliterans, periarteritis nodosa, systemic lupus erythematosus, rheumatic vascular disease and giant cell arteritis.", "contents": "Familial unusual encephalopathy of Binswanger's type without hypertension. Clinical and pathological studies have been conducted on two brothers with unusual encephalopathy of Binswanger's type. The disease started in the third decade with steady progressive course leading to death in eight or nine years. The clinical picture was summarized as a combination of organic dementia, extrapyramidal disorders associated with pseudobulbar symptoms and marked pyramidal tract signs. The blood pressure remained always normal during the course. Pathologically, there were diffuse and focal demyelination with sparing of U-fibers, multiple small foci of perivascular softening in the cerebral white matter and in the basal ganglia and severe arteriosclerotic changes of memingeal small arteries and long arteries with 100 to 400 micron caliber in the cerebral white matter. Vessel changes consisted of fibrous intimal proliferation, severe hyalinosis and splitting of intima and/or internal elastic membrane. The histopathological process belonged to the category of subcortical arteriosclerotic encephalopathy of Binswanger's type. There has been some discussion as to differential diagnosis among various forms of vasculitis such as cerebral endangiitis obliterans, periarteritis nodosa, systemic lupus erythematosus, rheumatic vascular disease and giant cell arteritis.", "PMID": 971885}
{"id": "pubmed23n0032_8719", "title": "Electron microscopic studies of the T-system--the effects of hyperosmolar glycerol and dantrolene sodium on mammalian muscles.", "content": "Electron microscopic studies of the T-systems in mammalian muscles were performed to investigate the effects of hyperosmolar glycerol solution and dantrolene sodium. The results indicate that in mammalian muscles the terminal cisterns of the sarcoplasmic reticulum seem to be the site of morphological changes which occur when the muscle specimens were transferred from the hypertonic glycerol solution to isotonic washing solution. When the muscle specimen was further exposed to dantrolene solution, the above changes were intensified. The changes of the terminal cisterns of SR were characterized by prominent vacuolar changes and the T-tubules were pressed and narrowed by those vacuoles secondarily. The glycerol and dantrolene technique did not cause any electron microscopic changes in the sarcolemma, myofibrils, nor Z-discs, and selective changes in the T-system, i.e. at the terminal cisterns of SR were demonstrated. This technique, excitation-contraction uncoupling, abolishes isometric twitch tension up to 99.7% and serves for the purpose of prolonged intracellular recordings of mammalian muscles without movement induced artifacts.", "contents": "Electron microscopic studies of the T-system--the effects of hyperosmolar glycerol and dantrolene sodium on mammalian muscles. Electron microscopic studies of the T-systems in mammalian muscles were performed to investigate the effects of hyperosmolar glycerol solution and dantrolene sodium. The results indicate that in mammalian muscles the terminal cisterns of the sarcoplasmic reticulum seem to be the site of morphological changes which occur when the muscle specimens were transferred from the hypertonic glycerol solution to isotonic washing solution. When the muscle specimen was further exposed to dantrolene solution, the above changes were intensified. The changes of the terminal cisterns of SR were characterized by prominent vacuolar changes and the T-tubules were pressed and narrowed by those vacuoles secondarily. The glycerol and dantrolene technique did not cause any electron microscopic changes in the sarcolemma, myofibrils, nor Z-discs, and selective changes in the T-system, i.e. at the terminal cisterns of SR were demonstrated. This technique, excitation-contraction uncoupling, abolishes isometric twitch tension up to 99.7% and serves for the purpose of prolonged intracellular recordings of mammalian muscles without movement induced artifacts.", "PMID": 971886}
{"id": "pubmed23n0032_8720", "title": "Immunoglobulin abnormalities in epileptic patients treated with diphenylhydantoin.", "content": "An immunologic study was made on 170 epileptic patients treated with antiepileptic drugs. The most noteworthy finding was that the serum IgA was decreased in 22 patients, and an IgA deficiency noticed in 12 of them. The blastic transformation rate of lymphocytes in PHA-containing cultures appeared low in seven out of 12 patients. Other findings included decreased serum IgM and IgG in some patients. These abnormalities were not encountered in epileptic patients taking no anti-epileptic drugs. Therefore, they could be considered as the side effects of antiepileptic drugs. In all patients manifesting these abnormalities, diphenylhydantoin was used in common. It was surmised that the above-mentioned immunologic abnormalities caused by anti-epileptic drugs, especially diphenylhydantoin, had resulted from disorders of the antibody producing organs in the bone marrow-thymus-lymphatic system.", "contents": "Immunoglobulin abnormalities in epileptic patients treated with diphenylhydantoin. An immunologic study was made on 170 epileptic patients treated with antiepileptic drugs. The most noteworthy finding was that the serum IgA was decreased in 22 patients, and an IgA deficiency noticed in 12 of them. The blastic transformation rate of lymphocytes in PHA-containing cultures appeared low in seven out of 12 patients. Other findings included decreased serum IgM and IgG in some patients. These abnormalities were not encountered in epileptic patients taking no anti-epileptic drugs. Therefore, they could be considered as the side effects of antiepileptic drugs. In all patients manifesting these abnormalities, diphenylhydantoin was used in common. It was surmised that the above-mentioned immunologic abnormalities caused by anti-epileptic drugs, especially diphenylhydantoin, had resulted from disorders of the antibody producing organs in the bone marrow-thymus-lymphatic system.", "PMID": 971887}
{"id": "pubmed23n0032_8721", "title": "[Characterization of active fetal movements in early pregnancy using ultrasound (time-motion technic)].", "content": "In 82 pregnancies between 9 and 14 weeks of amenorrhea, 120 registrations of active movements of the embryo were recorded by the ultrasonic time motion technique. Four types of early motility could be observed: Ia = single convulsions of the body, Ib = series of body convulsions, II = rapid movements of the extremities, III = combined complexe movements. The frequency and complexity of the movements increased with progressing gestational age.", "contents": "[Characterization of active fetal movements in early pregnancy using ultrasound (time-motion technic)]. In 82 pregnancies between 9 and 14 weeks of amenorrhea, 120 registrations of active movements of the embryo were recorded by the ultrasonic time motion technique. Four types of early motility could be observed: Ia = single convulsions of the body, Ib = series of body convulsions, II = rapid movements of the extremities, III = combined complexe movements. The frequency and complexity of the movements increased with progressing gestational age.", "PMID": 971889}
{"id": "pubmed23n0032_8722", "title": "[Intensive care in obstetrics and gynecology. Indications for transfer to the intensive care unit].", "content": "Between the years of 1969 and 1974, 68 patients were transferred from our Department of Obstetrics and Gynecology to the Intensive Care Unit. This figure represents 0,5% of all operative cases. 50% were high risk patients, previously under the care of our physicians. 11,8% occurred because of problems arising from latent diseases not previously diagnosed. The main reasons for transfer were septic shock, coagulopathy arising from abruptio placenta, placenta praevia, embolism and pneumonia. Interdepartmental cooperation is indispensable for correct diagnosis and a timely transfer.", "contents": "[Intensive care in obstetrics and gynecology. Indications for transfer to the intensive care unit]. Between the years of 1969 and 1974, 68 patients were transferred from our Department of Obstetrics and Gynecology to the Intensive Care Unit. This figure represents 0,5% of all operative cases. 50% were high risk patients, previously under the care of our physicians. 11,8% occurred because of problems arising from latent diseases not previously diagnosed. The main reasons for transfer were septic shock, coagulopathy arising from abruptio placenta, placenta praevia, embolism and pneumonia. Interdepartmental cooperation is indispensable for correct diagnosis and a timely transfer.", "PMID": 971890}
{"id": "pubmed23n0032_8723", "title": "[Obese patients in obstetrics].", "content": "The results of two studies about the course of pregnancy and delivery in adipose women in our hospital are combined and discussed. We found a higher rate of EPH-gestosis in overweight patients. The frequency of Cesarean section was increased. Belated uterine involution post partum is more frequent in adipose women, also the occurrence of urinary tract infections. There is a significant increase in perinatal mortality, mainly due to an increase in still-born. Pneumonia due to aspiration and birth traumata occur more frequently as well and endanger the children.", "contents": "[Obese patients in obstetrics]. The results of two studies about the course of pregnancy and delivery in adipose women in our hospital are combined and discussed. We found a higher rate of EPH-gestosis in overweight patients. The frequency of Cesarean section was increased. Belated uterine involution post partum is more frequent in adipose women, also the occurrence of urinary tract infections. There is a significant increase in perinatal mortality, mainly due to an increase in still-born. Pneumonia due to aspiration and birth traumata occur more frequently as well and endanger the children.", "PMID": 971892}
{"id": "pubmed23n0032_8724", "title": "[Complications following ambulatory abortion].", "content": "A survey was made of 394 abortions with ensuing complications during the years 1970-1974. Of these 17 were induced abortions, 3 of which showed serious complications. In two of these cases uterus extirpation by laparotomy was necessary.", "contents": "[Complications following ambulatory abortion]. A survey was made of 394 abortions with ensuing complications during the years 1970-1974. Of these 17 were induced abortions, 3 of which showed serious complications. In two of these cases uterus extirpation by laparotomy was necessary.", "PMID": 971896}
{"id": "pubmed23n0032_8725", "title": "[Zeiss Axiomat - a new microscope system].", "content": "A new microscope concept is described whereby the individual main functions of a universal microscope are performed by box-type, square-based modules which can be combined at will. The main characteristics of the new Axiomat microscope are the quality of its optics, its universality and stability.", "contents": "[Zeiss Axiomat - a new microscope system]. A new microscope concept is described whereby the individual main functions of a universal microscope are performed by box-type, square-based modules which can be combined at will. The main characteristics of the new Axiomat microscope are the quality of its optics, its universality and stability.", "PMID": 971897}
{"id": "pubmed23n0032_8726", "title": "[Epidemiology of endometrial hyperplasia and adenocarcinoma].", "content": "In a retrospective study characteristics of 729 climacteric and postmenopausal women with hyperplasia and adenocarcinoma of the endometrium are compared with those of 82 women with atrophic endometrium and 96 women with carcinoma of the cervix. In a prospective study 225 women with glandular-cystic, adenomatous and atypical hyperplasia of the endometrium have been checked by a control-curettage within a period of two months until four years following the first diagnosis. Low parity, disturbances of menstruation with anovulatoric bleedings during fertility period, adipositas, hypertension and diabetes mellitus in climacteric and postmenopausal women indicate a high risk of carcinoma of the endometrium. Hyperplasias of the endometrium in climacteric women cannot be considered as precursors of corpus carcinoma. They are the result of a temporary hormonal dysfunction. Prophylactic hysterectomy, however, should be performed, if adenomatous or atypical hyperplasia appears in older postmenopausal women with the indicators of high risk of endometriumcarcinoma as mentioned above.", "contents": "[Epidemiology of endometrial hyperplasia and adenocarcinoma]. In a retrospective study characteristics of 729 climacteric and postmenopausal women with hyperplasia and adenocarcinoma of the endometrium are compared with those of 82 women with atrophic endometrium and 96 women with carcinoma of the cervix. In a prospective study 225 women with glandular-cystic, adenomatous and atypical hyperplasia of the endometrium have been checked by a control-curettage within a period of two months until four years following the first diagnosis. Low parity, disturbances of menstruation with anovulatoric bleedings during fertility period, adipositas, hypertension and diabetes mellitus in climacteric and postmenopausal women indicate a high risk of carcinoma of the endometrium. Hyperplasias of the endometrium in climacteric women cannot be considered as precursors of corpus carcinoma. They are the result of a temporary hormonal dysfunction. Prophylactic hysterectomy, however, should be performed, if adenomatous or atypical hyperplasia appears in older postmenopausal women with the indicators of high risk of endometriumcarcinoma as mentioned above.", "PMID": 971898}
{"id": "pubmed23n0032_8727", "title": "[Seat belts and pregnancy].", "content": "The best safety for the mother and her unborn child while driving a car is the proper use of a seat belt. Only a correctly constructed and fitted lap-shoulder safety harness should be used. The instruction to wear a seat belt and to wear it correctly should be part of the pregnancy-care-program for each physician.", "contents": "[Seat belts and pregnancy]. The best safety for the mother and her unborn child while driving a car is the proper use of a seat belt. Only a correctly constructed and fitted lap-shoulder safety harness should be used. The instruction to wear a seat belt and to wear it correctly should be part of the pregnancy-care-program for each physician.", "PMID": 971900}
{"id": "pubmed23n0032_8728", "title": "Perspectives on accountability in mental health and retardation services.", "content": "Public mental health and mental retardation agencies are under increasing pressure to demonstrate their accountability in fiscal, legal, program, and other areas. The author discusses some of the problems and issures in pinpointing accountability and identifies eight areas of accountability--to patients, to program and management staff, to a chief executive, to a legislative body, to the public, to one's profession, and to one's self. He describes ways in which accountability can be demonstrated and emphasizes that, for mental health professionals, the overriding issue in accountability must be the provision of high-quality care and treatment for the mentally ill and mentally retarded.", "contents": "Perspectives on accountability in mental health and retardation services. Public mental health and mental retardation agencies are under increasing pressure to demonstrate their accountability in fiscal, legal, program, and other areas. The author discusses some of the problems and issures in pinpointing accountability and identifies eight areas of accountability--to patients, to program and management staff, to a chief executive, to a legislative body, to the public, to one's profession, and to one's self. He describes ways in which accountability can be demonstrated and emphasizes that, for mental health professionals, the overriding issue in accountability must be the provision of high-quality care and treatment for the mentally ill and mentally retarded.", "PMID": 971906}
{"id": "pubmed23n0032_8729", "title": "State hospital review boards in Minnesota.", "content": "For the past eight years Minnesota has had a review board for each state hospital serving the mentally ill and the mentally retarded. Each board consists of at least three individuals appointed by the commissioner of the department of public welfare. The board is an external review body in that members are not associated with the department or with the state hospital. It visits the hospital as frequently as required to review commitment papers and processes, assess the propriety of treatment procedures, ensure that each patient has an individualized treatment plan, interview patients and record their concerns, conduct a random review of patients' records, and meet with hospital staff. The board reports its findings to the commissioner and to the head of the hospital.", "contents": "State hospital review boards in Minnesota. For the past eight years Minnesota has had a review board for each state hospital serving the mentally ill and the mentally retarded. Each board consists of at least three individuals appointed by the commissioner of the department of public welfare. The board is an external review body in that members are not associated with the department or with the state hospital. It visits the hospital as frequently as required to review commitment papers and processes, assess the propriety of treatment procedures, ensure that each patient has an individualized treatment plan, interview patients and record their concerns, conduct a random review of patients' records, and meet with hospital staff. The board reports its findings to the commissioner and to the head of the hospital.", "PMID": 971907}
{"id": "pubmed23n0032_8730", "title": "A psychiatrist in a bureaucracy: the unsettling compromises.", "content": "Psychiatrists employed by bureaucracies must make a number of compromises in order to reconcile their employing organization's formally stated aims, their superiors' day-to-day expectations, and their traditional role model of physician and patient-oriented therapist. The author studied nine psychiatrists working for the U.S. Army during a two-year period in the mid-seventies. Their adaptation to expectations and pressures of the bureaucracy can be described in one of three general ways: through alignment with the official views of the Army, compartmentalization of roles to meet both patients' expectations and the Army's expectations, or rejection of a military identity.", "contents": "A psychiatrist in a bureaucracy: the unsettling compromises. Psychiatrists employed by bureaucracies must make a number of compromises in order to reconcile their employing organization's formally stated aims, their superiors' day-to-day expectations, and their traditional role model of physician and patient-oriented therapist. The author studied nine psychiatrists working for the U.S. Army during a two-year period in the mid-seventies. Their adaptation to expectations and pressures of the bureaucracy can be described in one of three general ways: through alignment with the official views of the Army, compartmentalization of roles to meet both patients' expectations and the Army's expectations, or rejection of a military identity.", "PMID": 971908}
{"id": "pubmed23n0032_8731", "title": "The politics of program evaluation: the mental health experience.", "content": "The authors discuss evaluation studies of the community mental health centers program. The emphasize that program evaluation is a political activity to influence the allocation of public resources. They examine its relationship to decision-making, power conflicts, and bureaucratic goals. They point out that the evaluator is seldom totally objective. They also emphasize the increasing use of evaluation by politicians.", "contents": "The politics of program evaluation: the mental health experience. The authors discuss evaluation studies of the community mental health centers program. The emphasize that program evaluation is a political activity to influence the allocation of public resources. They examine its relationship to decision-making, power conflicts, and bureaucratic goals. They point out that the evaluator is seldom totally objective. They also emphasize the increasing use of evaluation by politicians.", "PMID": 971909}
{"id": "pubmed23n0032_8732", "title": "Psychiatric peer review: a working model.", "content": "The author describes a system for psychiatric peer review developed by a peer review committee in a general hospital. At the heart of the system is a form called the peer review check sheet, which requires the physician to specify diagnosis, reasons for hospitalization, and major symptom complexes, as well as laboratory work and treatment and discharge plans. Minimal criteria have been established to verify the diagnoses and to justify the need for hospitalization. Each month the check sheets are removed from the charts of patients who have been discharged and sent to committee members for evaluation. Those that are incomplete or unsatisfactory signal the need to examine the patient's chart.", "contents": "Psychiatric peer review: a working model. The author describes a system for psychiatric peer review developed by a peer review committee in a general hospital. At the heart of the system is a form called the peer review check sheet, which requires the physician to specify diagnosis, reasons for hospitalization, and major symptom complexes, as well as laboratory work and treatment and discharge plans. Minimal criteria have been established to verify the diagnoses and to justify the need for hospitalization. Each month the check sheets are removed from the charts of patients who have been discharged and sent to committee members for evaluation. Those that are incomplete or unsatisfactory signal the need to examine the patient's chart.", "PMID": 971910}
{"id": "pubmed23n0032_8733", "title": "Ribonucleic acid synthesis in normal and immune macrophages after antigenic stimulus.", "content": "Macrophage ribonucleic acid (RNA) synthesis is an important metabolic process intimately related to the function of these cells. Mouse peritoneal macrophage RNA was extracted with phenol in the presence of bentonite and electrophoresed on composite agarose-polyacrylamide gels. The pulse-chase technique was used to follow the precursor relationships in macrophage ribosomal RNA (rRNA) maturation. The rRNA species at 18S and 28S appeared at 15 and 45 min, respectively, after RNA synthesis was halted. Their appearance corresponded closely to decreases in the rRNA precursors at 45S, 36S, and 34S. Studies of RNA methylation aided in confirming the identity of these ribosomal species. Unmethylated RNA species appeared as messenger RNA between 5S and 15S, and at about 55S probably represented heterodisperse nuclear RNA. When normal macrophages were incubated with heat-killed Salmonella enteritidis, an acceleration in the maturation of RNA was observed. The accelerated maturation was indicated by the earlier appearance of 28S rRNA and the more rapid development of an equilibrium state, where further labeling did not change the RNA profile. In macrophage RNA from mice immunized with S. enteritidis, rRNA species appeared rapidly but did not accumulate to the same extent as observed for normal macrophages. Precursor rRNA and other RNA species developed as usual, suggesting specific degradation of mature rRNA. Such rRNA wastage could indicate a mechanism controlling ribosome assembly in the non-proliferating activated macrophage. The pattern of RNA synthesis in immune macrophages was essentially unchanged by the presence of heat-killed S. enteritidis in vitro.", "contents": "Ribonucleic acid synthesis in normal and immune macrophages after antigenic stimulus. Macrophage ribonucleic acid (RNA) synthesis is an important metabolic process intimately related to the function of these cells. Mouse peritoneal macrophage RNA was extracted with phenol in the presence of bentonite and electrophoresed on composite agarose-polyacrylamide gels. The pulse-chase technique was used to follow the precursor relationships in macrophage ribosomal RNA (rRNA) maturation. The rRNA species at 18S and 28S appeared at 15 and 45 min, respectively, after RNA synthesis was halted. Their appearance corresponded closely to decreases in the rRNA precursors at 45S, 36S, and 34S. Studies of RNA methylation aided in confirming the identity of these ribosomal species. Unmethylated RNA species appeared as messenger RNA between 5S and 15S, and at about 55S probably represented heterodisperse nuclear RNA. When normal macrophages were incubated with heat-killed Salmonella enteritidis, an acceleration in the maturation of RNA was observed. The accelerated maturation was indicated by the earlier appearance of 28S rRNA and the more rapid development of an equilibrium state, where further labeling did not change the RNA profile. In macrophage RNA from mice immunized with S. enteritidis, rRNA species appeared rapidly but did not accumulate to the same extent as observed for normal macrophages. Precursor rRNA and other RNA species developed as usual, suggesting specific degradation of mature rRNA. Such rRNA wastage could indicate a mechanism controlling ribosome assembly in the non-proliferating activated macrophage. The pattern of RNA synthesis in immune macrophages was essentially unchanged by the presence of heat-killed S. enteritidis in vitro.", "PMID": 971940}
{"id": "pubmed23n0032_8734", "title": "Immunoglobulin classes in genital secretions of mycoplasma-infected and normal heifers.", "content": "Immunoglobulin levels were studied in the genital secretions of seven heifers which had been bred to a bull infected with Mycoplasma agalactiae var. bovis and three heifers bred to a noninfected bull. The median immunoglobulin G (IgG)/IgA ratio ratio for vaginal secretions was 0.7, for cervical secretions 21.9, and for uterine secretions 13.0. This indicated that IgA was the mahor immunoglobilin class in the most superficial portion of the reproductive tract and that IgG was the major class in secretions of the deeper tract. The response to infection was studied by comparing animals infected with mycoplasma and noninfected controls. The IgG/IgA ratios were significantly lower in infected animals than in controls, apparently due to high IgA values in infected animals. Low mycoplasmal agglutinin titers were detected in secretions of three of the infected animals, but there appeared to be no relationship between agglutinin titers and histopathological lesions or between agglutinin titers and IgG/IgA ratios in this group of animals.", "contents": "Immunoglobulin classes in genital secretions of mycoplasma-infected and normal heifers. Immunoglobulin levels were studied in the genital secretions of seven heifers which had been bred to a bull infected with Mycoplasma agalactiae var. bovis and three heifers bred to a noninfected bull. The median immunoglobulin G (IgG)/IgA ratio ratio for vaginal secretions was 0.7, for cervical secretions 21.9, and for uterine secretions 13.0. This indicated that IgA was the mahor immunoglobilin class in the most superficial portion of the reproductive tract and that IgG was the major class in secretions of the deeper tract. The response to infection was studied by comparing animals infected with mycoplasma and noninfected controls. The IgG/IgA ratios were significantly lower in infected animals than in controls, apparently due to high IgA values in infected animals. Low mycoplasmal agglutinin titers were detected in secretions of three of the infected animals, but there appeared to be no relationship between agglutinin titers and histopathological lesions or between agglutinin titers and IgG/IgA ratios in this group of animals.", "PMID": 971941}
{"id": "pubmed23n0032_8735", "title": "Inactivation of hemolytic complement in human serum by an acylated polysaccharide from a gram-positive rod: possible significance in pigeon-breeder's disease.", "content": "In an attempt to identify the major complement-consuming heteropolysaccharides in pigeon excreta, a number of teichoic acids and lipoteichoic acids from gram-positive bacteria were examined as possible substitutes. Teichoic acids from the cell wall and membrane of Lactobacillus buchnerii were found suitable. Samples of freshly voided pigeon droppings were cultured under anaerobic conditions and the bacteria were identified, subcultured, and extracted with phenol-water. Cell wall and membrane antigens from isolated Lactobacillus species could replace pigeon excreta antigens in diagnostic complement consumption tests. Purification and chemical characterization indicated the major antigen from Lactobacillus in this respect to be an acylated heteropolysaccharide.", "contents": "Inactivation of hemolytic complement in human serum by an acylated polysaccharide from a gram-positive rod: possible significance in pigeon-breeder's disease. In an attempt to identify the major complement-consuming heteropolysaccharides in pigeon excreta, a number of teichoic acids and lipoteichoic acids from gram-positive bacteria were examined as possible substitutes. Teichoic acids from the cell wall and membrane of Lactobacillus buchnerii were found suitable. Samples of freshly voided pigeon droppings were cultured under anaerobic conditions and the bacteria were identified, subcultured, and extracted with phenol-water. Cell wall and membrane antigens from isolated Lactobacillus species could replace pigeon excreta antigens in diagnostic complement consumption tests. Purification and chemical characterization indicated the major antigen from Lactobacillus in this respect to be an acylated heteropolysaccharide.", "PMID": 971942}
{"id": "pubmed23n0032_8736", "title": "Endotoxic activity of rough organisms of Brucella species.", "content": "A rough-specific antigen extracted from the rough species Brucella ovis and lipopolysaccharide extracted from smooth Brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. Acetone-extracted whole cells of B. ovis and of B. canis and of a rough mutant of B; abortus had the same toxicity for mice, but it was not possible to extract endotoxin from B. canis by the methods used.", "contents": "Endotoxic activity of rough organisms of Brucella species. A rough-specific antigen extracted from the rough species Brucella ovis and lipopolysaccharide extracted from smooth Brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. Acetone-extracted whole cells of B. ovis and of B. canis and of a rough mutant of B; abortus had the same toxicity for mice, but it was not possible to extract endotoxin from B. canis by the methods used.", "PMID": 971943}
{"id": "pubmed23n0032_8737", "title": "T2 lipopolysaccharide antigen of Salmonella: genetic determination of T2 and properties of the T2, T2,S, and T2,SR Forms.", "content": "The T2 antigenic form of Salmonella bareilly was examined. The absence of O specificity in this strain was shown to be due to its nonfunctional rfb genes; when the rfb gene cluster was replaced by the rfb cluster derived from smooth donor strains, T2,S and T2,SR recombinants were produced that expressed both T2 and either 0-6,7 or 0-4,12 specificity, depending on O antigen of the donor strain. The T2, T2,S, and T2,SR forms were all unstable on culture and segregated T2-negative forms (R, S, and  SR, respectively) at a high rate. In all these respects the T2 antigen closely resembled the other T-form antigen, T1. The genes responsible for the T2 antigen, rfu, were not close to rfb, but their precise location and relation to rft (which determines T1 antigen) could not be discovered because of the instability to the T2 form and low recombination frequency in the necessary interspecies crosses.", "contents": "T2 lipopolysaccharide antigen of Salmonella: genetic determination of T2 and properties of the T2, T2,S, and T2,SR Forms. The T2 antigenic form of Salmonella bareilly was examined. The absence of O specificity in this strain was shown to be due to its nonfunctional rfb genes; when the rfb gene cluster was replaced by the rfb cluster derived from smooth donor strains, T2,S and T2,SR recombinants were produced that expressed both T2 and either 0-6,7 or 0-4,12 specificity, depending on O antigen of the donor strain. The T2, T2,S, and T2,SR forms were all unstable on culture and segregated T2-negative forms (R, S, and  SR, respectively) at a high rate. In all these respects the T2 antigen closely resembled the other T-form antigen, T1. The genes responsible for the T2 antigen, rfu, were not close to rfb, but their precise location and relation to rft (which determines T1 antigen) could not be discovered because of the instability to the T2 form and low recombination frequency in the necessary interspecies crosses.", "PMID": 971944}
{"id": "pubmed23n0032_8738", "title": "Lysis of Acholeplasma laidlawii by antibodies and complement.", "content": "Membranes of Acholeplasma laidlawii were used to determine the membrane components involved in immune lysis and to eventually detect enzymatic changes in the membrane components during this reaction. In a previous publication we reported that A. laidlawii can be killed by antibody and complement and that antibodies to membrane proteins are by far more effective than antibodies to membrane lipids in the complement-dependent killing of this organism. In this report we demonstrate that membrane damage occurs after the combined action of antibody and complement. In addition, the cytoplasmic enzyme hexokinase is released during immune killing. As visualized by electron microscopy, the organisms lost their cytoplasm and were transformed into ghosts. In the majority of organisms, tears in the membrane could be seen. Ultrastructural lesions of about 8.0 to 10 nm in diameter were visible when the organisms were incubated with antiserum and complement. 14C-labeled fatty acids were incorporated during growth into A. laidlawii membrane lipids. After incubation of labeled organisms with antiserum and complement, release of radioactive material into the supernatant and changes in the lipid composition were not observeed. Enzymatic degradation of membrane lipids of A. laidlawii during immune lysis was not detected.", "contents": "Lysis of Acholeplasma laidlawii by antibodies and complement. Membranes of Acholeplasma laidlawii were used to determine the membrane components involved in immune lysis and to eventually detect enzymatic changes in the membrane components during this reaction. In a previous publication we reported that A. laidlawii can be killed by antibody and complement and that antibodies to membrane proteins are by far more effective than antibodies to membrane lipids in the complement-dependent killing of this organism. In this report we demonstrate that membrane damage occurs after the combined action of antibody and complement. In addition, the cytoplasmic enzyme hexokinase is released during immune killing. As visualized by electron microscopy, the organisms lost their cytoplasm and were transformed into ghosts. In the majority of organisms, tears in the membrane could be seen. Ultrastructural lesions of about 8.0 to 10 nm in diameter were visible when the organisms were incubated with antiserum and complement. 14C-labeled fatty acids were incorporated during growth into A. laidlawii membrane lipids. After incubation of labeled organisms with antiserum and complement, release of radioactive material into the supernatant and changes in the lipid composition were not observeed. Enzymatic degradation of membrane lipids of A. laidlawii during immune lysis was not detected.", "PMID": 971945}
{"id": "pubmed23n0032_8739", "title": "Rosette-forming cells during immune response to Toxoplasma gondii in mice.", "content": "The rosette-forming cell (RFC) response of mice immunized with varying doses of Toxoplasma gondii was studied by immunocytoadherence (ICA). The specificity of ICA in the present system was tested by passive sensitization with hyperimmune serum in vivo and in vitro. A slight increase in RFC was observed with the latter. Prior treatment of spleen cells from immunized animals with rabbit anti-mouse immunoglobulin resulted in total inhibition of ICA. During the primary and the secondary response after 10 days, the number of RFC rose rapidly to reach the peak on the 3rd day. With secondary immunization 30 days later, the peak shifted to the 2nd day. Mice infected with a lower dose of Toxoplasma had a greater number of RFC during the secondary response after 10 days than with a larger dose.", "contents": "Rosette-forming cells during immune response to Toxoplasma gondii in mice. The rosette-forming cell (RFC) response of mice immunized with varying doses of Toxoplasma gondii was studied by immunocytoadherence (ICA). The specificity of ICA in the present system was tested by passive sensitization with hyperimmune serum in vivo and in vitro. A slight increase in RFC was observed with the latter. Prior treatment of spleen cells from immunized animals with rabbit anti-mouse immunoglobulin resulted in total inhibition of ICA. During the primary and the secondary response after 10 days, the number of RFC rose rapidly to reach the peak on the 3rd day. With secondary immunization 30 days later, the peak shifted to the 2nd day. Mice infected with a lower dose of Toxoplasma had a greater number of RFC during the secondary response after 10 days than with a larger dose.", "PMID": 971946}
{"id": "pubmed23n0032_8740", "title": "Further characterization of a lipopolysaccharide from Coxiella burneti.", "content": "The lipopolysaccharide previously isolated from the rickettsial agent of Q fever, Coxiella burneti, phase I, has been further characterized. The sugar residues ribose, mannose, gluclose, D-glycero-D-mannoheptose, and L-glycerto-D-mannoheptose are present. Two sugars remain unidentified, one of which is a minor and the other a major constituent. Isomyristic, palmitic, and beta-hydroxymyristic acids are the major fatty acid residues of the 15 identified. The nature and content of other lipopolysaccharide constituents are presented.", "contents": "Further characterization of a lipopolysaccharide from Coxiella burneti. The lipopolysaccharide previously isolated from the rickettsial agent of Q fever, Coxiella burneti, phase I, has been further characterized. The sugar residues ribose, mannose, gluclose, D-glycero-D-mannoheptose, and L-glycerto-D-mannoheptose are present. Two sugars remain unidentified, one of which is a minor and the other a major constituent. Isomyristic, palmitic, and beta-hydroxymyristic acids are the major fatty acid residues of the 15 identified. The nature and content of other lipopolysaccharide constituents are presented.", "PMID": 971947}
{"id": "pubmed23n0032_8741", "title": "Diffusible viral interference during arbovirus plaque formation.", "content": "A radially diffusing zone of nonspecific interference was observed surrounding plaques of Western equine encephalitis virus. The increase in diameters of the zones of interference were linear and more rapid than increases in plaque diameters. These observations raise the possibility that viral-induced interference could account for the diminished growth of Western equine encephalitis virus plaques with time or even the initial formation of plaques. In addition, this system could be used as a model to study localized host defenses during early infection of solid tissues in vivo.", "contents": "Diffusible viral interference during arbovirus plaque formation. A radially diffusing zone of nonspecific interference was observed surrounding plaques of Western equine encephalitis virus. The increase in diameters of the zones of interference were linear and more rapid than increases in plaque diameters. These observations raise the possibility that viral-induced interference could account for the diminished growth of Western equine encephalitis virus plaques with time or even the initial formation of plaques. In addition, this system could be used as a model to study localized host defenses during early infection of solid tissues in vivo.", "PMID": 971948}
{"id": "pubmed23n0032_8742", "title": "Differential inhibition of Streptococcus mutans in vitro adherence by anti-glucosyltransferase antibodies.", "content": "Antibodies prepared against an insoluble-soluble glucan-synthesizing fraction significantly inhibited in vitro adherence of Streptococcus mutans, whereas antibodies directed against a soluble glucan-synthesizing fraction were much less inhibitory.", "contents": "Differential inhibition of Streptococcus mutans in vitro adherence by anti-glucosyltransferase antibodies. Antibodies prepared against an insoluble-soluble glucan-synthesizing fraction significantly inhibited in vitro adherence of Streptococcus mutans, whereas antibodies directed against a soluble glucan-synthesizing fraction were much less inhibitory.", "PMID": 971949}
{"id": "pubmed23n0032_8743", "title": "Naturally occurring secretory immunoglobulin A antibodies to Streptococcus mutans in human colostrum and saliva.", "content": "Human colostrum, parotid saliva, and serum were assayed for the presence of naturally occurring antibodies to five serotypes of Streptococcus mutans. Appreciable levels of agglutinins to strains AHT, BHT, 10449, 6715, and LM-7 (groups a leads to e, respectively) were detected in normal colostrum and saliva, whereas relatively low levels were found in serum. No agglutinins could be detected in the colostrum or saliva of immunodeficient patients. Molecular sieve chromatography of the colostrum on Sephadex G-200 revealed agglutinin activity in the secretory immunoglobulin A (s-IgA)-rich fraction only. Titration of purified colostral s-IgA confirmed the IgA nature of this agglutinating activity. Indirect immunofluorescence tests with anti-s-IgA, -IgG, and -IgM revealed S. mutans specificity only in the s-IgA class. The presence of s-IgA antibodies to indigenous oral microorganisms in colostrum, as well as in saliva, suggests that antigenic stimulation occurs at a site remote from the oral mucosa.", "contents": "Naturally occurring secretory immunoglobulin A antibodies to Streptococcus mutans in human colostrum and saliva. Human colostrum, parotid saliva, and serum were assayed for the presence of naturally occurring antibodies to five serotypes of Streptococcus mutans. Appreciable levels of agglutinins to strains AHT, BHT, 10449, 6715, and LM-7 (groups a leads to e, respectively) were detected in normal colostrum and saliva, whereas relatively low levels were found in serum. No agglutinins could be detected in the colostrum or saliva of immunodeficient patients. Molecular sieve chromatography of the colostrum on Sephadex G-200 revealed agglutinin activity in the secretory immunoglobulin A (s-IgA)-rich fraction only. Titration of purified colostral s-IgA confirmed the IgA nature of this agglutinating activity. Indirect immunofluorescence tests with anti-s-IgA, -IgG, and -IgM revealed S. mutans specificity only in the s-IgA class. The presence of s-IgA antibodies to indigenous oral microorganisms in colostrum, as well as in saliva, suggests that antigenic stimulation occurs at a site remote from the oral mucosa.", "PMID": 971950}
{"id": "pubmed23n0032_8744", "title": "Cell-mediated immunity to Vibrio cholerae with ribonucleic acid-protein fractions of V. cholerae L-form lysates.", "content": "Different L-form lysate vaccines of Vibrio cholerae serotypes Ogawa and Inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (E-RNA) and phenol-extracted RNA (P-RNA) fractions of V. cholerae Ogawa lysates were tested for production of cell-mediated immunity. Both E-RNA and P-RNA fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. They also induced delayed hypersensitivity in rabbits. More consistent results were obtained with the P-RNA fraction.", "contents": "Cell-mediated immunity to Vibrio cholerae with ribonucleic acid-protein fractions of V. cholerae L-form lysates. Different L-form lysate vaccines of Vibrio cholerae serotypes Ogawa and Inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (E-RNA) and phenol-extracted RNA (P-RNA) fractions of V. cholerae Ogawa lysates were tested for production of cell-mediated immunity. Both E-RNA and P-RNA fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. They also induced delayed hypersensitivity in rabbits. More consistent results were obtained with the P-RNA fraction.", "PMID": 971951}
{"id": "pubmed23n0032_8745", "title": "Cell-mediated immune response to products of Actinomyces viscosus cultures.", "content": "Hartley strain guinea pigs were sensitized with 0.5 ml of concentrated cell-free Actinomyces viscosus culture supernatant fluids mixed with Freund complete adjuvant. Fourteen to 16 days later the animals were challenged by intradermal injection with 0.1 ml of the culture supernatant, and the reactions were observed at 4, 8, 16, 24, and 48 h. Peritoneal exudate cells from sensitized animals were used for determination of migration inhibition factor, and guinea pig peripheral blood served as a source of cells for determining the induction of mitogenesis by antigenic material. Skin responses were consistently positive to challenge with the test material, whereas reactions to noninoculated culture medium were negative. Sensitized cells, challenged with antigen, resulted in 60% or greater inhibition of migration of indicator cells in migration inhibition factor experiments. Tests for mitogenesis showed a greater than fourfold increase in isotope uptake when sensitized cells were challenged with test material. The data are consistent with the suggestion that A. viscosus culture supernatants contain substances that induce cell-mediated immune responses in guinea pigs.", "contents": "Cell-mediated immune response to products of Actinomyces viscosus cultures. Hartley strain guinea pigs were sensitized with 0.5 ml of concentrated cell-free Actinomyces viscosus culture supernatant fluids mixed with Freund complete adjuvant. Fourteen to 16 days later the animals were challenged by intradermal injection with 0.1 ml of the culture supernatant, and the reactions were observed at 4, 8, 16, 24, and 48 h. Peritoneal exudate cells from sensitized animals were used for determination of migration inhibition factor, and guinea pig peripheral blood served as a source of cells for determining the induction of mitogenesis by antigenic material. Skin responses were consistently positive to challenge with the test material, whereas reactions to noninoculated culture medium were negative. Sensitized cells, challenged with antigen, resulted in 60% or greater inhibition of migration of indicator cells in migration inhibition factor experiments. Tests for mitogenesis showed a greater than fourfold increase in isotope uptake when sensitized cells were challenged with test material. The data are consistent with the suggestion that A. viscosus culture supernatants contain substances that induce cell-mediated immune responses in guinea pigs.", "PMID": 971952}
{"id": "pubmed23n0032_8746", "title": "Peptido polysaccharide antigens from Trichophyton mentagrophytes var. granulosum.", "content": "Two highly purified peptido polysaccharide antigens have been isolated from surface-grown cultures of Trichophyton mentagrophytes var. granulosum. Trichloroacetic acid extraction and ethanol precipitation yielded a mixture containing high-molecular-weight components which were first separated on Sephadex G-200. Subsequent fractionation by ion-exchange chromatography on DE-52-cellulose (borate form) yielded the two peptido polysaccharides. Both of the peptido polysaccharides reacted with rabbit antiserum to T. mentagrophytes var. granulosum. The two peptido polysaccharides contain 73.2% hexoses (mannose-galactose-glucose, 7.5:0.7:1), 8.6% amino acids and 1.8% amino sugars and 77.4% hexoses (mannose-galactose-glucose, 9:0.3:1), 6.2% amino acids, and 0.4% amino sugars, respectively. Each contains 16 different amino acids, threonine, proline, and serine predominating.", "contents": "Peptido polysaccharide antigens from Trichophyton mentagrophytes var. granulosum. Two highly purified peptido polysaccharide antigens have been isolated from surface-grown cultures of Trichophyton mentagrophytes var. granulosum. Trichloroacetic acid extraction and ethanol precipitation yielded a mixture containing high-molecular-weight components which were first separated on Sephadex G-200. Subsequent fractionation by ion-exchange chromatography on DE-52-cellulose (borate form) yielded the two peptido polysaccharides. Both of the peptido polysaccharides reacted with rabbit antiserum to T. mentagrophytes var. granulosum. The two peptido polysaccharides contain 73.2% hexoses (mannose-galactose-glucose, 7.5:0.7:1), 8.6% amino acids and 1.8% amino sugars and 77.4% hexoses (mannose-galactose-glucose, 9:0.3:1), 6.2% amino acids, and 0.4% amino sugars, respectively. Each contains 16 different amino acids, threonine, proline, and serine predominating.", "PMID": 971953}
{"id": "pubmed23n0032_8747", "title": "Occurrence and distribution of sucrose-metabolizing enzymes in oral streptococci.", "content": "Specific growth rates, growth yields, and the level and cellular distribution of three sucrose-metabolizing enzyme activities were determined for seven oral streptococci (Streptococcus mutans strains E49, BHT, 10449, SL-1, and LM-7, S. sanguis 10558, and S. salivarius 25975). Cultures were grown in a fermentor at pH 6 with either 20 mM glucose or 10 mM sucrose. Generation times varied between 21 and 70 min. Whereas some strains grew 10 to 50% more slowly with sucrose than with glucose, others did not. Growth was always logarithmic, and the growth yields were similar. Glcosyl transferase (EC 2.4.1.5) was largely extracellular; in sucrose cultures it was appreciably lower, but no major shift to a cell-associated form was found. In glucose cultures, the activity varied between 4 and 140 IU per 6-liter culture. The glucan formed was mostly or exclusively water insoluble. Glcosyl transferase was stimulated weakly (60% or less) by various dextrans. Fructosyl transferase (EC 2.4.1.10) was primarily extracellular (except in glucose cultures of S. salivarius) and varied between 0 and 337 IU/culture. In S. salivarius, the extracellular fructosyl transferase was induced by sucrose. In all S. Mutans cultures, the total fructosyl transferase activity was lower after growth with sucrose. All strains had extra- and intracellular invertase (EC 3.2.1.26) activity. Total levels varied between 210 and 3,500 IU/culture. Less extracellular activity was present in sucrose cultures. Only S. salivarius had appreciable activity in the cellular particulate fraction. Invertase activity was significantly higher than the combined glucosyl and fructosyl transferase activities in all cultures.", "contents": "Occurrence and distribution of sucrose-metabolizing enzymes in oral streptococci. Specific growth rates, growth yields, and the level and cellular distribution of three sucrose-metabolizing enzyme activities were determined for seven oral streptococci (Streptococcus mutans strains E49, BHT, 10449, SL-1, and LM-7, S. sanguis 10558, and S. salivarius 25975). Cultures were grown in a fermentor at pH 6 with either 20 mM glucose or 10 mM sucrose. Generation times varied between 21 and 70 min. Whereas some strains grew 10 to 50% more slowly with sucrose than with glucose, others did not. Growth was always logarithmic, and the growth yields were similar. Glcosyl transferase (EC 2.4.1.5) was largely extracellular; in sucrose cultures it was appreciably lower, but no major shift to a cell-associated form was found. In glucose cultures, the activity varied between 4 and 140 IU per 6-liter culture. The glucan formed was mostly or exclusively water insoluble. Glcosyl transferase was stimulated weakly (60% or less) by various dextrans. Fructosyl transferase (EC 2.4.1.10) was primarily extracellular (except in glucose cultures of S. salivarius) and varied between 0 and 337 IU/culture. In S. salivarius, the extracellular fructosyl transferase was induced by sucrose. In all S. Mutans cultures, the total fructosyl transferase activity was lower after growth with sucrose. All strains had extra- and intracellular invertase (EC 3.2.1.26) activity. Total levels varied between 210 and 3,500 IU/culture. Less extracellular activity was present in sucrose cultures. Only S. salivarius had appreciable activity in the cellular particulate fraction. Invertase activity was significantly higher than the combined glucosyl and fructosyl transferase activities in all cultures.", "PMID": 971954}
{"id": "pubmed23n0032_8748", "title": "Interaction between Coxiella burnetii and guinea pig peritoneal macrophages.", "content": "The phagocytosis and subsequent degradation of phase I and II Coxiella burnetii by macrophages obtained from immune and nonimmune guinea pigs were compared. Phase I rickettsiae were more resistant to phagocytosis than were phase II organisms. There was no significant difference in the percentage of phagocytosis of either phase of rickettsiae by macrophages from immune or nonimmune animals. After ingestion, phase I and II organisms pretreated with normal serum multiplied and destroyed normal macrophages as well as macrophages obtained from guinea pigs immunized with phase II rickettsiae. In contrast, only phase I organisms were degraded by macrophages from phase I-immunized animals in the presence of normal serum. Immune serum rendered rickettsiae more susceptible to phagocytosis and also potentiated the destruction of organisms by all types of macrophages. The specificity of macrophages from phase I animals to degrade only phase I rickettsiae was demonstrated by the ability of Rickettsia rickettsii to replicate in these macrophages.", "contents": "Interaction between Coxiella burnetii and guinea pig peritoneal macrophages. The phagocytosis and subsequent degradation of phase I and II Coxiella burnetii by macrophages obtained from immune and nonimmune guinea pigs were compared. Phase I rickettsiae were more resistant to phagocytosis than were phase II organisms. There was no significant difference in the percentage of phagocytosis of either phase of rickettsiae by macrophages from immune or nonimmune animals. After ingestion, phase I and II organisms pretreated with normal serum multiplied and destroyed normal macrophages as well as macrophages obtained from guinea pigs immunized with phase II rickettsiae. In contrast, only phase I organisms were degraded by macrophages from phase I-immunized animals in the presence of normal serum. Immune serum rendered rickettsiae more susceptible to phagocytosis and also potentiated the destruction of organisms by all types of macrophages. The specificity of macrophages from phase I animals to degrade only phase I rickettsiae was demonstrated by the ability of Rickettsia rickettsii to replicate in these macrophages.", "PMID": 971955}
{"id": "pubmed23n0032_8749", "title": "Antibodies to two immunotypes of Chlamydia trachomatis in individuals with trachoma.", "content": "In Tunisia (North Africa), trachoma remains a common eye disease. Most cases are caused by immunotype A of Chlamydia trachomatis. In a small proportion of cases (less than 10%), type-specific antibodies to both immunotype A and immunotype B exist in the serum of patients. These types do not crossreact, and occasionally sequential acquisition of antibodies has been demonstrated. Thus, individuals in one endemic area of trachoma may be infected with two immunotypes of C. trachomatis. The epidemiological and immunological implications of this new finding are briefly discussed.", "contents": "Antibodies to two immunotypes of Chlamydia trachomatis in individuals with trachoma. In Tunisia (North Africa), trachoma remains a common eye disease. Most cases are caused by immunotype A of Chlamydia trachomatis. In a small proportion of cases (less than 10%), type-specific antibodies to both immunotype A and immunotype B exist in the serum of patients. These types do not crossreact, and occasionally sequential acquisition of antibodies has been demonstrated. Thus, individuals in one endemic area of trachoma may be infected with two immunotypes of C. trachomatis. The epidemiological and immunological implications of this new finding are briefly discussed.", "PMID": 971956}
{"id": "pubmed23n0032_8750", "title": "Serum-free culture of hamster lymphoid cells and differential inhibition of lipopolysaccharide stimulation by isologous serum.", "content": "The optimal conditions for serum-free cultures of hamster lymphoid cells were determined. The cells responded to both the nonspecific thymus-derived lymphocyte mitogen concanavalin A (Con A) and the nonspecific bone marrow-derived lymphocyte mitogens lipopolysaccharide (LPS) and dextran sulfate (DxS). Normal MHA hamster serum was shown to specifically inhibit the response to LPS and DxS by 95% without inhibiting the response to Con A. The serum also did not inhibit one-way mixed lymphocyte reaction. Incubation of cells in 5% serum for short periods of time, followed by serum-free culture, did not lead to inhibition. The serum inhibited the LPS response by 65% even when added 24 h after initiation of the culture. The activity was associated with the high-molecular-weight material on Sephadex G-200 fractionation of serum. The inhibitory fraction did not possess antibody activity to LPS. The possibility that the material is an alpha2-macroglobulin is discussed.", "contents": "Serum-free culture of hamster lymphoid cells and differential inhibition of lipopolysaccharide stimulation by isologous serum. The optimal conditions for serum-free cultures of hamster lymphoid cells were determined. The cells responded to both the nonspecific thymus-derived lymphocyte mitogen concanavalin A (Con A) and the nonspecific bone marrow-derived lymphocyte mitogens lipopolysaccharide (LPS) and dextran sulfate (DxS). Normal MHA hamster serum was shown to specifically inhibit the response to LPS and DxS by 95% without inhibiting the response to Con A. The serum also did not inhibit one-way mixed lymphocyte reaction. Incubation of cells in 5% serum for short periods of time, followed by serum-free culture, did not lead to inhibition. The serum inhibited the LPS response by 65% even when added 24 h after initiation of the culture. The activity was associated with the high-molecular-weight material on Sephadex G-200 fractionation of serum. The inhibitory fraction did not possess antibody activity to LPS. The possibility that the material is an alpha2-macroglobulin is discussed.", "PMID": 971957}
{"id": "pubmed23n0032_8751", "title": "Inherited resistance to Corynebacterium kutscheri in mice.", "content": "An analysis of the factors responsible for inherited resistance to Corynebacterium kutscheri was undertaken. Various inbred mouse strains were examined; these included the Swiss Lynch and C57Bl/l mice, their F1 and F2 progeny, and the progeny of the F1 backcrossed to each parent strain. Two modes of inherited resistance are described. An examination suggested that resistance as measured by the mean lethal dose of C. kutscheri was under polygenic control and was inherited continuously. However, the efficiency with which C. kutscheri was eliminated by the mononuclear phagocyte cells of the liver over 3 days differed markedly among strains. A genetic analysis of this mononuclear phagocyte microbicidal efficiency (MPME) in Swiss Lynch and C57Bl/6 mice was undertaken. The trait, MPME, was present, but did not segregate, in the F1 progeny or in the progeny of the backcross to the resistant C57Bl/6 parent; this was clear evidence of dominance. Moreover, MPME segregated in a ratio of 1:1 in the progeny of the backcross to the sensitive Swiss Lynch parent and in a ratio of 3:1 in the F2 progeny. It was concluded that MPME was inherited discontinuously and was controlled by a single dominant autosomal gene (or closely linked group); the recessive allele was assigned the gene symbol ack. Linkage experiments showed there to be no association between the ack locus and any of the immune-response genes.", "contents": "Inherited resistance to Corynebacterium kutscheri in mice. An analysis of the factors responsible for inherited resistance to Corynebacterium kutscheri was undertaken. Various inbred mouse strains were examined; these included the Swiss Lynch and C57Bl/l mice, their F1 and F2 progeny, and the progeny of the F1 backcrossed to each parent strain. Two modes of inherited resistance are described. An examination suggested that resistance as measured by the mean lethal dose of C. kutscheri was under polygenic control and was inherited continuously. However, the efficiency with which C. kutscheri was eliminated by the mononuclear phagocyte cells of the liver over 3 days differed markedly among strains. A genetic analysis of this mononuclear phagocyte microbicidal efficiency (MPME) in Swiss Lynch and C57Bl/6 mice was undertaken. The trait, MPME, was present, but did not segregate, in the F1 progeny or in the progeny of the backcross to the resistant C57Bl/6 parent; this was clear evidence of dominance. Moreover, MPME segregated in a ratio of 1:1 in the progeny of the backcross to the sensitive Swiss Lynch parent and in a ratio of 3:1 in the F2 progeny. It was concluded that MPME was inherited discontinuously and was controlled by a single dominant autosomal gene (or closely linked group); the recessive allele was assigned the gene symbol ack. Linkage experiments showed there to be no association between the ack locus and any of the immune-response genes.", "PMID": 971958}
{"id": "pubmed23n0032_8752", "title": "Extracellular and bacterial factors influencing staphylococcal phagocytosis and killing by human polymorphonuclear leukocytes.", "content": "Extracellular and bacterial factors that influence the phagocytosis and killing of staphylococci by human polymorphonuclear leukocytes have been studied. Staphylococcus epidermidis strains were, in general, more rapidly phagocytized than were S. aureus strains. However, two strains of S. epidermidis had a very slow rate of ingestion. Although the rate of phagocytosis of S. aureus Wood 46 was greater than that of S. aureus 502A, the Wood 46 strain was more difficult to kill. Serum was essential for phagocytosis of both S. aureus and S. epidermidis. The opsonic titer of pooled serum was similar for S. aureus and S. epidermidis. In normal pooled serum, heat-labile factors were more important for effective phagocytosis than they were in immune serum. Although a saturation point for ingestion was reached, the percentage of ingested bacteria that remained alive within the leukocyte remained relatively fixed. Heat-killed and live staphylococci were igested in a similar fashion. The rate of phagocytosis was greatly reduced at 41 degrees C.", "contents": "Extracellular and bacterial factors influencing staphylococcal phagocytosis and killing by human polymorphonuclear leukocytes. Extracellular and bacterial factors that influence the phagocytosis and killing of staphylococci by human polymorphonuclear leukocytes have been studied. Staphylococcus epidermidis strains were, in general, more rapidly phagocytized than were S. aureus strains. However, two strains of S. epidermidis had a very slow rate of ingestion. Although the rate of phagocytosis of S. aureus Wood 46 was greater than that of S. aureus 502A, the Wood 46 strain was more difficult to kill. Serum was essential for phagocytosis of both S. aureus and S. epidermidis. The opsonic titer of pooled serum was similar for S. aureus and S. epidermidis. In normal pooled serum, heat-labile factors were more important for effective phagocytosis than they were in immune serum. Although a saturation point for ingestion was reached, the percentage of ingested bacteria that remained alive within the leukocyte remained relatively fixed. Heat-killed and live staphylococci were igested in a similar fashion. The rate of phagocytosis was greatly reduced at 41 degrees C.", "PMID": 971959}
{"id": "pubmed23n0032_8753", "title": "Induction of cellular and humoral immunological responsiveness to a soluble cercarial antigen preparation from Schistosoma mansoni.", "content": "The development of immunological responsiveness to a soluble cercarial antigenic preparation (CAP) from Schistosoma mansoni was analyzed in inbred CBA/J mice infected with cercariae, one or multiple times, or sensitized using CAP. Repeated exposure to 75 cercariae at three weekly intervals (3X-75) or subcutaneous administration of 20 mug of CAP (CAP/complete Freund adjuvant [CFA]) stimulated the development of specific anti-CAP lymph node cell blastogenesis. The degree of responsiveness was dependent upon the concentration of CAP in the culture system and was optimal in the dose range of 20 to 30 mug of protein of CAP per culture. Animals exposed once to 75 or 225 cercariae or to two sequential weekly infections with 75 cercariae exhibited a minimal response to CAP in comparison to the responsiveness of 3X-75 or CAP/CFA lymph node cells. Assessment of anti-CAP agglutinating antibody by application of a microtiter passive hemagglutination technique revealed that both 3X-75 and CAP/CFA animals possessed low titers of activity. In addition, both 3X-75 and CAP/CFA sera contained reagin-like antibodies to CAP as detected by the heterologous (rat), 72-h latent period, passive cutaneous anaphylaxis technique.", "contents": "Induction of cellular and humoral immunological responsiveness to a soluble cercarial antigen preparation from Schistosoma mansoni. The development of immunological responsiveness to a soluble cercarial antigenic preparation (CAP) from Schistosoma mansoni was analyzed in inbred CBA/J mice infected with cercariae, one or multiple times, or sensitized using CAP. Repeated exposure to 75 cercariae at three weekly intervals (3X-75) or subcutaneous administration of 20 mug of CAP (CAP/complete Freund adjuvant [CFA]) stimulated the development of specific anti-CAP lymph node cell blastogenesis. The degree of responsiveness was dependent upon the concentration of CAP in the culture system and was optimal in the dose range of 20 to 30 mug of protein of CAP per culture. Animals exposed once to 75 or 225 cercariae or to two sequential weekly infections with 75 cercariae exhibited a minimal response to CAP in comparison to the responsiveness of 3X-75 or CAP/CFA lymph node cells. Assessment of anti-CAP agglutinating antibody by application of a microtiter passive hemagglutination technique revealed that both 3X-75 and CAP/CFA animals possessed low titers of activity. In addition, both 3X-75 and CAP/CFA sera contained reagin-like antibodies to CAP as detected by the heterologous (rat), 72-h latent period, passive cutaneous anaphylaxis technique.", "PMID": 971960}
{"id": "pubmed23n0032_8754", "title": "Analysis of the intradermal response against a soluble cercarial antigenic preparation from Schistosoma mansoni.", "content": "Intradermal testing was performed with a soluble cercarial antigenic preparation (CAP) from Schistosoma mansoni cercariae in CBA/J mice multiply infected with S. mansoni or sensitized with CAP. Both an early (5-h) response and a late (24- to 48-h) reaction to CAP, as measured by increase in dermal thickness, was elicited after injection of antigen into the ears of either multiply infected (3X-75) or CAP-sensitized (CAP/complete Freund adjuvant [CFA]) mice. Histopathological examination showed that the early response was primarily vascular in nature and involved a polymorphonuclear cell infiltrate in and around dilated capillaries. The late reaction to CAP consisted of a perivascular cellular infiltrate of polymorphonuclear and mononuclear cell types. Passive transfer of 3X-75-infected or CAP/CFA-sensitized serum (0.4 ml) to normal mice conveyed the ability to mount an early (5-h) response to CAP which was marked histopathologically by a prominent polymorphonuclear cell infiltrate. The majority of the responsiveness in normal mice after administration of lymph node cells (40 X 10(6)) from multiply infected or CAP/CFA-sensitized mice was observed 24 to 48 h after injection of CAP and was mononuclear in nature.", "contents": "Analysis of the intradermal response against a soluble cercarial antigenic preparation from Schistosoma mansoni. Intradermal testing was performed with a soluble cercarial antigenic preparation (CAP) from Schistosoma mansoni cercariae in CBA/J mice multiply infected with S. mansoni or sensitized with CAP. Both an early (5-h) response and a late (24- to 48-h) reaction to CAP, as measured by increase in dermal thickness, was elicited after injection of antigen into the ears of either multiply infected (3X-75) or CAP-sensitized (CAP/complete Freund adjuvant [CFA]) mice. Histopathological examination showed that the early response was primarily vascular in nature and involved a polymorphonuclear cell infiltrate in and around dilated capillaries. The late reaction to CAP consisted of a perivascular cellular infiltrate of polymorphonuclear and mononuclear cell types. Passive transfer of 3X-75-infected or CAP/CFA-sensitized serum (0.4 ml) to normal mice conveyed the ability to mount an early (5-h) response to CAP which was marked histopathologically by a prominent polymorphonuclear cell infiltrate. The majority of the responsiveness in normal mice after administration of lymph node cells (40 X 10(6)) from multiply infected or CAP/CFA-sensitized mice was observed 24 to 48 h after injection of CAP and was mononuclear in nature.", "PMID": 971961}
{"id": "pubmed23n0032_8755", "title": "Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies.", "content": "Colonization of the intestinal epithelium by Vibrio cholerae was examined in two model systems, in ligated ileal loops of adult rabbits and in the patent gut of infant rabbits, using both scanning and transmission electron microscopy. Time studies in the adult model showed a lag period of up to 1 h before the attachment of significant numbers of the vibrios. The bacteria appeared initially in small patches on the sides of the villi, predominantly along the transverse furrows. The number of adherent bacteria steadily increased, reaching a maximum between 4 and 7 h, when a dense mat of bacteria several layers thick covered much of the villi. After this time there was a rapid decline in the number of V. cholerae bound. By 12 to 16 h only a few bacteria could be seen on the surface of the villi, which had a rough, patchy appearance at these later times. Globular protrusions, with vibrios attached, may play a role in the clearance of bacteria. Colonization and clearance in the patent intestine of the infant rabbit occurred much as in the adult model. However, the bacteria adhered more uniformly and there was no lag in attachment. In both models the majority of bacteria were aligned horizontally with the epithelial surface, but some were attached in an end-on manner, with their flagella extending into the lumen. The bacteria adhered via their surface coats directly to the tips of the microvilli, except for a few vibrios that were partly embedded into the brush border. Some changes in the microvilli occurred as a consequence of the bacterial attachment.", "contents": "Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies. Colonization of the intestinal epithelium by Vibrio cholerae was examined in two model systems, in ligated ileal loops of adult rabbits and in the patent gut of infant rabbits, using both scanning and transmission electron microscopy. Time studies in the adult model showed a lag period of up to 1 h before the attachment of significant numbers of the vibrios. The bacteria appeared initially in small patches on the sides of the villi, predominantly along the transverse furrows. The number of adherent bacteria steadily increased, reaching a maximum between 4 and 7 h, when a dense mat of bacteria several layers thick covered much of the villi. After this time there was a rapid decline in the number of V. cholerae bound. By 12 to 16 h only a few bacteria could be seen on the surface of the villi, which had a rough, patchy appearance at these later times. Globular protrusions, with vibrios attached, may play a role in the clearance of bacteria. Colonization and clearance in the patent intestine of the infant rabbit occurred much as in the adult model. However, the bacteria adhered more uniformly and there was no lag in attachment. In both models the majority of bacteria were aligned horizontally with the epithelial surface, but some were attached in an end-on manner, with their flagella extending into the lumen. The bacteria adhered via their surface coats directly to the tips of the microvilli, except for a few vibrios that were partly embedded into the brush border. Some changes in the microvilli occurred as a consequence of the bacterial attachment.", "PMID": 971962}
{"id": "pubmed23n0032_8756", "title": "In vivo and in vitro studies on possible pathogenic mechanisms of Actinomyces viscosus.", "content": "Actinomycotic infections are characterized by long-term inflammatory lesions containing large numbers of polymorphonuclear leukocytes (PMNs) and mononuclear cells. The pathogenic mechanisms involved in these lesions are not understood. Homogenates of Actinomyces viscosus (AVIS) induce an acute inflammatory response with a predominance of PMNs within 6 h after injection into the footpads of nonimmunized mice. These homogenates, when tested in vitro, contain potent chemotactic activity for human PMNs. In vitro chemotactic activity for human monocytes is weak but statistically significant (P less than 0.025). Doses of AVIS, which alone have little chemotactic activity, cause the generation of PMN chemotactic activity in fresh, but not complement-inactivated, serum. The injection of AVIS into the footpads of immunized mice induces an acute inflammatory response followed within 48 h by a mononuclear cell infiltrate, suggesting that factors affecting monocyte accumulation are generated by the immune host in response to challenge with the bacterial antigens. These findings indicate that the pathogenicity of the Actinomyces may result in part from (i) their direct chemotactic effect on PMNs, (ii) their cytotaxigenic effects on serum, and (iii) their ability to stimulate host immune cells to produce and release mediators of inflammation.", "contents": "In vivo and in vitro studies on possible pathogenic mechanisms of Actinomyces viscosus. Actinomycotic infections are characterized by long-term inflammatory lesions containing large numbers of polymorphonuclear leukocytes (PMNs) and mononuclear cells. The pathogenic mechanisms involved in these lesions are not understood. Homogenates of Actinomyces viscosus (AVIS) induce an acute inflammatory response with a predominance of PMNs within 6 h after injection into the footpads of nonimmunized mice. These homogenates, when tested in vitro, contain potent chemotactic activity for human PMNs. In vitro chemotactic activity for human monocytes is weak but statistically significant (P less than 0.025). Doses of AVIS, which alone have little chemotactic activity, cause the generation of PMN chemotactic activity in fresh, but not complement-inactivated, serum. The injection of AVIS into the footpads of immunized mice induces an acute inflammatory response followed within 48 h by a mononuclear cell infiltrate, suggesting that factors affecting monocyte accumulation are generated by the immune host in response to challenge with the bacterial antigens. These findings indicate that the pathogenicity of the Actinomyces may result in part from (i) their direct chemotactic effect on PMNs, (ii) their cytotaxigenic effects on serum, and (iii) their ability to stimulate host immune cells to produce and release mediators of inflammation.", "PMID": 971963}
{"id": "pubmed23n0032_8757", "title": "Lysis and killing of bacteria by lysosomal proteinases.", "content": "The bacteriolytic and bactericidal effects of the human proteinases cathepsin B, cathepsin D, cathepsin G, and elastase were investigated. Cathepsin G and elastase were 5 to 10% as active as egg white lysozyme in the lysis of Micrococcus lysodeikticus. All four enzymes slowly lysed the lysozyme-resistant Staphylococcus aureus. The gram-negative Acinetobacter 199A was rendered sensitive to lysozyme by all of the proteinases. Only elastase caused marked proteolysis of the outer membrane, which would permit access by lysozyme to the underlying peptidoglycan. When the surface layer of regularly arranged a protein was removed, however, the outer membrane proteins became susceptible to the other proteinases. Cathepsin G, elastase, and cathepsin D were bactericidal to Acinetobacter 199A. The bactericidal activity of cathepsin D was shown to be dependent on enzymatic activity, unlike that of cathepsin G, which was related to its cationic nature.", "contents": "Lysis and killing of bacteria by lysosomal proteinases. The bacteriolytic and bactericidal effects of the human proteinases cathepsin B, cathepsin D, cathepsin G, and elastase were investigated. Cathepsin G and elastase were 5 to 10% as active as egg white lysozyme in the lysis of Micrococcus lysodeikticus. All four enzymes slowly lysed the lysozyme-resistant Staphylococcus aureus. The gram-negative Acinetobacter 199A was rendered sensitive to lysozyme by all of the proteinases. Only elastase caused marked proteolysis of the outer membrane, which would permit access by lysozyme to the underlying peptidoglycan. When the surface layer of regularly arranged a protein was removed, however, the outer membrane proteins became susceptible to the other proteinases. Cathepsin G, elastase, and cathepsin D were bactericidal to Acinetobacter 199A. The bactericidal activity of cathepsin D was shown to be dependent on enzymatic activity, unlike that of cathepsin G, which was related to its cationic nature.", "PMID": 971964}
{"id": "pubmed23n0032_8758", "title": "Interferon production in mice by components of Salmonella minnesota R595 lipid A.", "content": "The lipid A precipitate obtained by partial hydrolysis in 1% acetic acid of the glycolipid of a mutant strain of Salmonella minnesota (rough strain R595) was separated into a number of components by thin-layer chromatography. When tested as bovine serum albumin complexes, the minimal interferon-inducing dose in mice of the parent glycolipid was 0.1 mug, whereas that of lipid A was 1.0 mug. The minimal dose of the separated components ranged from 0.1 to 20 mug. The components did not differ significantly in regard to glucosamine-phosphate-fatty acid ratios. The results further emphasize that lipid A is a heterogeneous substance that contains a variety of components of differing biological activity.", "contents": "Interferon production in mice by components of Salmonella minnesota R595 lipid A. The lipid A precipitate obtained by partial hydrolysis in 1% acetic acid of the glycolipid of a mutant strain of Salmonella minnesota (rough strain R595) was separated into a number of components by thin-layer chromatography. When tested as bovine serum albumin complexes, the minimal interferon-inducing dose in mice of the parent glycolipid was 0.1 mug, whereas that of lipid A was 1.0 mug. The minimal dose of the separated components ranged from 0.1 to 20 mug. The components did not differ significantly in regard to glucosamine-phosphate-fatty acid ratios. The results further emphasize that lipid A is a heterogeneous substance that contains a variety of components of differing biological activity.", "PMID": 971965}
{"id": "pubmed23n0032_8759", "title": "A comparative study of the economics of home care.", "content": "The costs of home care and treatment solely in hospital for patients in a variety of short-term diagnostic categories are compared. Five hundred and eighty-three patients included in an experimental home care program were randomly assigned either to a group which received home care as part of their treatment, or to a control group that remained in hospital the traditional length of time. It is argued that the only costs relevant in an economic comparison of the two modes of treatment are those attributable to the direct care of the patient. A technique is presented whereby changes in the daily amount of nursing service provided can be costed. The economic analysis shows that, when similar diagnoses are compared for an episode of illness, there is basically no difference in cost between home care and treatment in hospital.", "contents": "A comparative study of the economics of home care. The costs of home care and treatment solely in hospital for patients in a variety of short-term diagnostic categories are compared. Five hundred and eighty-three patients included in an experimental home care program were randomly assigned either to a group which received home care as part of their treatment, or to a control group that remained in hospital the traditional length of time. It is argued that the only costs relevant in an economic comparison of the two modes of treatment are those attributable to the direct care of the patient. A technique is presented whereby changes in the daily amount of nursing service provided can be costed. The economic analysis shows that, when similar diagnoses are compared for an episode of illness, there is basically no difference in cost between home care and treatment in hospital.", "PMID": 971966}
{"id": "pubmed23n0032_8760", "title": "The diffusion of new hospital technologies in the United States.", "content": "Increases in the real resources used in hospital care have been an important cause behind rising hospital costs in the United States. Many of these resources have taken the form of new hospital technologies, and this paper begins by reviewing the trends in adoption of new hospital technologies over the years 1950-1974. The resource requirements, costs , and to the extent possible the patient benefits, of two of these technologies are then discussed in more detail: intensive care, a widespread facility with many variations, has been a major contributor to hospital costs; radiotherapy has been characterized by a succession of competing technologies. Regulatory efforts such as certificiate-of-need reviews would be more effective if they viewed hospitals as flexible collections of such technologies-with the costs and patient benefits of each to be weighed separately-rather than primarily in terms of numbers of beds. A national center to collect information on the separate technological functions of hospitals and make it available to interested groups would make a useful contribution to hospital regulation.", "contents": "The diffusion of new hospital technologies in the United States. Increases in the real resources used in hospital care have been an important cause behind rising hospital costs in the United States. Many of these resources have taken the form of new hospital technologies, and this paper begins by reviewing the trends in adoption of new hospital technologies over the years 1950-1974. The resource requirements, costs , and to the extent possible the patient benefits, of two of these technologies are then discussed in more detail: intensive care, a widespread facility with many variations, has been a major contributor to hospital costs; radiotherapy has been characterized by a succession of competing technologies. Regulatory efforts such as certificiate-of-need reviews would be more effective if they viewed hospitals as flexible collections of such technologies-with the costs and patient benefits of each to be weighed separately-rather than primarily in terms of numbers of beds. A national center to collect information on the separate technological functions of hospitals and make it available to interested groups would make a useful contribution to hospital regulation.", "PMID": 971967}
{"id": "pubmed23n0032_8761", "title": "The service economy and postindustrial society: a sociological critique.", "content": "The growth of the service sector in advanced capitalist societies is the empirical evidence in support of the ideological approach to our social systems as \"postindustrial.\" By examining the content of so-called \"service activities\" and by relating the observed trends to the model of uneven development in the U.S., this article tries to explain the transformation of the social structure of the U.S. on the basis of the contradications inherent in the processes of accumulation of capital, class struggle, and state intervention.", "contents": "The service economy and postindustrial society: a sociological critique. The growth of the service sector in advanced capitalist societies is the empirical evidence in support of the ideological approach to our social systems as \"postindustrial.\" By examining the content of so-called \"service activities\" and by relating the observed trends to the model of uneven development in the U.S., this article tries to explain the transformation of the social structure of the U.S. on the basis of the contradications inherent in the processes of accumulation of capital, class struggle, and state intervention.", "PMID": 971968}
{"id": "pubmed23n0032_8762", "title": "Breast-feeding: the role of multinational corporations in Latin America.", "content": "The decline in birthrates in the developed countries of the world has forced multinational corporations engaged in the production of infant formula to seek out new markets in the developing countries, where burgeoning population rates potentially guarantee the long-term profitability of these corporations. This development, ostensibly benign and nutritionally advantageous to infants in developing countries, has serious public health consequences, due to the high relative cost of purchased formula and the paucity of hygienic facilities essential to the sterile preparation of bottle formula. This paper delineates in detail economic and contraceptive advantages of breast-feeding, and examines the role of health personnel and multinational advertising techniques which have catalyzed the decline in breast-feeding. In addition, the paper focuses on the question of cultural imperialism and current efforts to regulate the multinational firms through both United Nations groups and stock-holders' suits. Finally, some suggestions are made concerning ameliorative public policy approaches to the breast-feeding controversy.", "contents": "Breast-feeding: the role of multinational corporations in Latin America. The decline in birthrates in the developed countries of the world has forced multinational corporations engaged in the production of infant formula to seek out new markets in the developing countries, where burgeoning population rates potentially guarantee the long-term profitability of these corporations. This development, ostensibly benign and nutritionally advantageous to infants in developing countries, has serious public health consequences, due to the high relative cost of purchased formula and the paucity of hygienic facilities essential to the sterile preparation of bottle formula. This paper delineates in detail economic and contraceptive advantages of breast-feeding, and examines the role of health personnel and multinational advertising techniques which have catalyzed the decline in breast-feeding. In addition, the paper focuses on the question of cultural imperialism and current efforts to regulate the multinational firms through both United Nations groups and stock-holders' suits. Finally, some suggestions are made concerning ameliorative public policy approaches to the breast-feeding controversy.", "PMID": 971969}
{"id": "pubmed23n0032_8763", "title": "Malpractice in the United Kingdom.", "content": "The end of the last century saw the development in the United Kingdom of medical protection and defense societies. Their history, objects, growth, and scope are outlined. Reference is made to leading cases of medical malpractice and to the relevant statutes. The relationship between the protection societies and the National Health Service authorities is dealt with and a comparison made of the relative situations in the United States and United Kingdom. Finally, recent trends are examined and views expressed about possible future developments in this field which will be the subject of recommendations by a Royal Commission on Civil Liability and Compensation for Personal Injury which is now sitting.", "contents": "Malpractice in the United Kingdom. The end of the last century saw the development in the United Kingdom of medical protection and defense societies. Their history, objects, growth, and scope are outlined. Reference is made to leading cases of medical malpractice and to the relevant statutes. The relationship between the protection societies and the National Health Service authorities is dealt with and a comparison made of the relative situations in the United States and United Kingdom. Finally, recent trends are examined and views expressed about possible future developments in this field which will be the subject of recommendations by a Royal Commission on Civil Liability and Compensation for Personal Injury which is now sitting.", "PMID": 971970}
{"id": "pubmed23n0032_8764", "title": "Nurses and trade unions in Britain.", "content": "The nature of the negotiating body for pay and conditions of nurses in Britain and some of the reasons for the body's past failure to negotiate a substantial pay increase for nurses are described in this article. A description is given of a series of events, including strike action by nurses during 1974, which led to a substantial pay raise. The pay award, however, represented only a partial success for the trade unions and the negotiating body. Methods of nurses' organization and the roles of their representative unions and professional body indicate some of the reasons for only a partial success. The lack of unity between the National Health Service trade unions and the reactionary role of the professional body were notable. However, many important political and organizational lessons were learned by nurses in this struggle. We are now facing savage cuts in National Health Service expenditure, leading to a reduction in the number of nurses employed. These lessons are clearly useful for the nurses and their organization with the labor movement in fighting these cuts in National Health Service expenditure.", "contents": "Nurses and trade unions in Britain. The nature of the negotiating body for pay and conditions of nurses in Britain and some of the reasons for the body's past failure to negotiate a substantial pay increase for nurses are described in this article. A description is given of a series of events, including strike action by nurses during 1974, which led to a substantial pay raise. The pay award, however, represented only a partial success for the trade unions and the negotiating body. Methods of nurses' organization and the roles of their representative unions and professional body indicate some of the reasons for only a partial success. The lack of unity between the National Health Service trade unions and the reactionary role of the professional body were notable. However, many important political and organizational lessons were learned by nurses in this struggle. We are now facing savage cuts in National Health Service expenditure, leading to a reduction in the number of nurses employed. These lessons are clearly useful for the nurses and their organization with the labor movement in fighting these cuts in National Health Service expenditure.", "PMID": 971971}
{"id": "pubmed23n0032_8765", "title": "Observations on potential contributions of health planning.", "content": "The passage of the National Health Planning and Resources Development Act in the United States in 1974 is used to set the context for a new assessment of health planning as a change agent. In reviewing the record of health planning the most striking conclusion is that even its friends have been unable to establish that it has had any quantifiable impact. The authors suggest, however, that comprehensive health planning may have stimulated the belief that changes in medical care organization are crucial to improving the health care system. The authors next consider the role of health planning inferred from three widely espoused \"models\" of the health care system: professional, central planning, and market. Although market advocates generally deemphasize health planning as contrasted to those supporting a centrally planned system, none of the models is sufficiently developed to indicate specific roles and functions fro health planning. Basing their argument on goals for health care reform generally espoused by students of medical care organization, the authors assert that health planning agenices will be most effective if they are organizationally linked to general-purpose governments, encourage the formation of Health Maintenance Organizations, consciously involve themselves in health system reorganization, and design their policies so they can be effectively evaluated.", "contents": "Observations on potential contributions of health planning. The passage of the National Health Planning and Resources Development Act in the United States in 1974 is used to set the context for a new assessment of health planning as a change agent. In reviewing the record of health planning the most striking conclusion is that even its friends have been unable to establish that it has had any quantifiable impact. The authors suggest, however, that comprehensive health planning may have stimulated the belief that changes in medical care organization are crucial to improving the health care system. The authors next consider the role of health planning inferred from three widely espoused \"models\" of the health care system: professional, central planning, and market. Although market advocates generally deemphasize health planning as contrasted to those supporting a centrally planned system, none of the models is sufficiently developed to indicate specific roles and functions fro health planning. Basing their argument on goals for health care reform generally espoused by students of medical care organization, the authors assert that health planning agenices will be most effective if they are organizationally linked to general-purpose governments, encourage the formation of Health Maintenance Organizations, consciously involve themselves in health system reorganization, and design their policies so they can be effectively evaluated.", "PMID": 971972}
{"id": "pubmed23n0032_8766", "title": "Toward the formulation of sociodental indicators.", "content": "The bases for the construction of sociodental indicators is discussed in the paper, considering several available indexes of oral health status (dental caries, periodontal disease, malocclusion, oral hygiene, and other oral conditions) as well as measures of quality of services. Very little research exists relating any of the above measures to social indicators such as personal life-style or cultural and ecological factors. Such expansion would enable dental indicators to be useful for purposes of policy decisions. Combining any dental indicators or set of indicators with a potential global social health index is discussed in terms of potential problems obscuring dentistry's cost to society. Dentistry, in addition, is offered as a system in microcosm-one which can be useful for purposes of polishing methodology for the social health indicator movement.", "contents": "Toward the formulation of sociodental indicators. The bases for the construction of sociodental indicators is discussed in the paper, considering several available indexes of oral health status (dental caries, periodontal disease, malocclusion, oral hygiene, and other oral conditions) as well as measures of quality of services. Very little research exists relating any of the above measures to social indicators such as personal life-style or cultural and ecological factors. Such expansion would enable dental indicators to be useful for purposes of policy decisions. Combining any dental indicators or set of indicators with a potential global social health index is discussed in terms of potential problems obscuring dentistry's cost to society. Dentistry, in addition, is offered as a system in microcosm-one which can be useful for purposes of polishing methodology for the social health indicator movement.", "PMID": 971976}
{"id": "pubmed23n0032_8767", "title": "Urticaria secondary to a copper intrauterine device.", "content": "A 24-year-old woman developed in acute urticarial reaction secondary to a copper intrauterine contraceptive device. Allergy to copper was proven by scratch tests. The condition cleared with removal of the IUD.", "contents": "Urticaria secondary to a copper intrauterine device. A 24-year-old woman developed in acute urticarial reaction secondary to a copper intrauterine contraceptive device. Allergy to copper was proven by scratch tests. The condition cleared with removal of the IUD.", "PMID": 971979}
{"id": "pubmed23n0032_8768", "title": "Preoperative evaluation of the strabismus patient.", "content": "The preoperative evaluation of the strabismus patient may require one or several examination sessions. Since general anesthesia is most often employed, the patient's general health, hematological and neurological status, and concurrent medications should be evaluated. The age of onset and whether the deviation was intermittent in character for some part of its course are important clues to fusion potential. The examination should emphasize close observation of the eyes when they are stationary and during rotations; it should be conducted so as to encourage, rather than frustrate, binocular cooperation. This requires test selection that takes into account both variations in alignment (at different test distances) and amblyopia. Sensory evaluation can involve many tests, but specifically for surgical planning it can be brief and apply principal to justification of surgery for small-angle, nonsymptomatic deviations. The phenomenon of intractable diplopia is unusual and probably should not be considered a strong deterrent to surgical correction of a long-standing and cosmetically unpleasant misalignment.", "contents": "Preoperative evaluation of the strabismus patient. The preoperative evaluation of the strabismus patient may require one or several examination sessions. Since general anesthesia is most often employed, the patient's general health, hematological and neurological status, and concurrent medications should be evaluated. The age of onset and whether the deviation was intermittent in character for some part of its course are important clues to fusion potential. The examination should emphasize close observation of the eyes when they are stationary and during rotations; it should be conducted so as to encourage, rather than frustrate, binocular cooperation. This requires test selection that takes into account both variations in alignment (at different test distances) and amblyopia. Sensory evaluation can involve many tests, but specifically for surgical planning it can be brief and apply principal to justification of surgery for small-angle, nonsymptomatic deviations. The phenomenon of intractable diplopia is unusual and probably should not be considered a strong deterrent to surgical correction of a long-standing and cosmetically unpleasant misalignment.", "PMID": 971982}
{"id": "pubmed23n0032_8769", "title": "Surgical weakening of the inferior oblique.", "content": "1. The inferior oblique may be weakened effectively by recession, disinsertion, or myectomy, disrupting the muscle continuity between Lockwood's ligament and the muscle's insertion. 2. A successful unilateral inferior oblique weakening produces 20 prism diopters less hypertropia or more hypotropia in the field of action and has less effect away from the muscle's field of action; a bilateral weakening of the inferior oblique produces 20 prism diopters less exotropia or more esotropia in upgaze. 3. Complications of inferior oblique weakening are persistent overaction, operation on the wrong muscle, and the adherence syndrome. 4. The adherence syndrome is not related to the myectomy procedure specifically but is probably related to (or caused by) fat rupture with hemorrhage, which may accompany any type of inferior oblique weakening. 5. The adherence syndrome can be avoided by careful surgical technique.", "contents": "Surgical weakening of the inferior oblique. 1. The inferior oblique may be weakened effectively by recession, disinsertion, or myectomy, disrupting the muscle continuity between Lockwood's ligament and the muscle's insertion. 2. A successful unilateral inferior oblique weakening produces 20 prism diopters less hypertropia or more hypotropia in the field of action and has less effect away from the muscle's field of action; a bilateral weakening of the inferior oblique produces 20 prism diopters less exotropia or more esotropia in upgaze. 3. Complications of inferior oblique weakening are persistent overaction, operation on the wrong muscle, and the adherence syndrome. 4. The adherence syndrome is not related to the myectomy procedure specifically but is probably related to (or caused by) fat rupture with hemorrhage, which may accompany any type of inferior oblique weakening. 5. The adherence syndrome can be avoided by careful surgical technique.", "PMID": 971984}
{"id": "pubmed23n0032_8770", "title": "Superior oblique palsy: diagnosis and classification.", "content": "A review of 130 cases of superior oblique palsy suggests that simple office procedures are sufficient to establish the diagnosis. Prism and cover test measurements with alternate eyes fixing in the nine diagnostic positions of gaze proved to be the greatest help in classifying these cases with regard to surgical planning.", "contents": "Superior oblique palsy: diagnosis and classification. A review of 130 cases of superior oblique palsy suggests that simple office procedures are sufficient to establish the diagnosis. Prism and cover test measurements with alternate eyes fixing in the nine diagnostic positions of gaze proved to be the greatest help in classifying these cases with regard to surgical planning.", "PMID": 971985}
{"id": "pubmed23n0032_8771", "title": "Diagnosis and surgical options in superior oblique surgery.", "content": "The surgical treatment of superior oblique paresis is most gratifying to the patient and to the surgeon. Whereas the large range of vertical fusion may make it difficult to bring out the total deviation, this same fusion is the surgeon's best ally postoperatively. In addition, while there are multiple choice as to which muscle or muscles to operate upon, the great advantages of the tucking operation on the superior oblique are (1) it practically always straightens the head, (2) it greatly reduces the hyperdeviation, and (3) it has no effect on the width of the lid fissures.", "contents": "Diagnosis and surgical options in superior oblique surgery. The surgical treatment of superior oblique paresis is most gratifying to the patient and to the surgeon. Whereas the large range of vertical fusion may make it difficult to bring out the total deviation, this same fusion is the surgeon's best ally postoperatively. In addition, while there are multiple choice as to which muscle or muscles to operate upon, the great advantages of the tucking operation on the superior oblique are (1) it practically always straightens the head, (2) it greatly reduces the hyperdeviation, and (3) it has no effect on the width of the lid fissures.", "PMID": 971986}
{"id": "pubmed23n0032_8772", "title": "Superior oblique tenotomy: indications and complications.", "content": "Two series of patients who had superior oblique tenotomies either alone or in combination with horizontal muscle surgery were evaluated, and the results and complications were analyzed. The effect of bilateral superior oblique tenotomies in downgaze correlated well with the previously reported series. In these two series, the effects in primary and upgaze also were evaluated. An average effect of 12 prism diopters eso-shift was found to occur in primary position, while no eso- or exo-shift was found in upgaze. Operative indications and contradictions, when followed carefully, make predictable the effectiveness of the operation in collapsing an A pattern. The reported unequal effects on superior oblique action were not observed in any of the patients studied. In no instance in these series was there a worsening of preexisting hypertropia or development of a new one. Transient sheathlike Brown's syndrome occurred postoperatively in some patients in the early series, but this problem was reduced by the instillation of steroid suspensions. V patterns developed postoperatively in 20 percent of the cases in the first series and in 9 percent in the second series. There was approximately a one-in-three chance that the inferior oblique muscle would become slightly to moderately overactive postoperatively. The incidence of this complication will be reduced significantly or eliminated by careful case selection to choose only those patients who have slightly underactive inferior oblique muscles preoperatively.", "contents": "Superior oblique tenotomy: indications and complications. Two series of patients who had superior oblique tenotomies either alone or in combination with horizontal muscle surgery were evaluated, and the results and complications were analyzed. The effect of bilateral superior oblique tenotomies in downgaze correlated well with the previously reported series. In these two series, the effects in primary and upgaze also were evaluated. An average effect of 12 prism diopters eso-shift was found to occur in primary position, while no eso- or exo-shift was found in upgaze. Operative indications and contradictions, when followed carefully, make predictable the effectiveness of the operation in collapsing an A pattern. The reported unequal effects on superior oblique action were not observed in any of the patients studied. In no instance in these series was there a worsening of preexisting hypertropia or development of a new one. Transient sheathlike Brown's syndrome occurred postoperatively in some patients in the early series, but this problem was reduced by the instillation of steroid suspensions. V patterns developed postoperatively in 20 percent of the cases in the first series and in 9 percent in the second series. There was approximately a one-in-three chance that the inferior oblique muscle would become slightly to moderately overactive postoperatively. The incidence of this complication will be reduced significantly or eliminated by careful case selection to choose only those patients who have slightly underactive inferior oblique muscles preoperatively.", "PMID": 971987}
{"id": "pubmed23n0032_8773", "title": "The role of the fascia in muscle surgery.", "content": "Mishandling of the fascial tissues during surgery on the extraocular muscles accounts for unsatisfactory alignment of the eye in the primary position; restricted movement of the globe; scarred, injected, heaped conjunctiva; and changes in the normal eyelid level. These hardships may be impossible to overcome by performing secondary surgery. The most serious and frequently recurring abuse of the fascia during muscle surgery is the needless penetration of the Tenon's capsule beyond 10 mm. from the limbus, which results in prolapse of the fat cushion into the spaces that are normally between the movable fascial planes and sclera. The prolapsed fat infiltrates into the sclera and muscle capsules and undergoes fibrofatty proliferation; with time, cicatrization of these scarred tissues pulls the eye to a deviated position, restricting its normal movement. Also, the fat cushion migrates forward under the conjunctiva toward the limbus, causing the unsightly appearance of injected yellowish fleshy tissue near the limbus. A patient's ocular motility can be ruined for life and his appearance deformed because of a surgeon's disrespect for the important role Tenon's capsule plays in the motility of the globe.", "contents": "The role of the fascia in muscle surgery. Mishandling of the fascial tissues during surgery on the extraocular muscles accounts for unsatisfactory alignment of the eye in the primary position; restricted movement of the globe; scarred, injected, heaped conjunctiva; and changes in the normal eyelid level. These hardships may be impossible to overcome by performing secondary surgery. The most serious and frequently recurring abuse of the fascia during muscle surgery is the needless penetration of the Tenon's capsule beyond 10 mm. from the limbus, which results in prolapse of the fat cushion into the spaces that are normally between the movable fascial planes and sclera. The prolapsed fat infiltrates into the sclera and muscle capsules and undergoes fibrofatty proliferation; with time, cicatrization of these scarred tissues pulls the eye to a deviated position, restricting its normal movement. Also, the fat cushion migrates forward under the conjunctiva toward the limbus, causing the unsightly appearance of injected yellowish fleshy tissue near the limbus. A patient's ocular motility can be ruined for life and his appearance deformed because of a surgeon's disrespect for the important role Tenon's capsule plays in the motility of the globe.", "PMID": 971989}
{"id": "pubmed23n0032_8774", "title": "Surgery of dissociated vertical divergent strabismus.", "content": "In those cases where definite bilateral inferior oblique overaciton coexists and predominates, the initial procedure should be a maximum weakening operation of both inferior oblique muscles. For the remainder, surgical treatment should be reserved for the unsightly predominantly unilateral cases who do not wish to wait for the possible beneficial effects of age. With surgery on the vertical rectus muscles, particularly the inferior rectus, improvement of the deviation by two-thirds to three-quarters should be the aim of treatment.", "contents": "Surgery of dissociated vertical divergent strabismus. In those cases where definite bilateral inferior oblique overaciton coexists and predominates, the initial procedure should be a maximum weakening operation of both inferior oblique muscles. For the remainder, surgical treatment should be reserved for the unsightly predominantly unilateral cases who do not wish to wait for the possible beneficial effects of age. With surgery on the vertical rectus muscles, particularly the inferior rectus, improvement of the deviation by two-thirds to three-quarters should be the aim of treatment.", "PMID": 971990}
{"id": "pubmed23n0032_8775", "title": "Role of Tenon's capsule in postoperative restrictions.", "content": "Management of all restricted strabismus cases is difficult and frequently will require two or more procedures. The prevention of these restrictions at the time of primary surgery is far more important. All tissue should be handled as gently as possible, with smooth or fine-tip forceps, avoiding large fixation devices. Sharp dissection is essential - tissue should not be torn but cut carefully and sharply with scissors under direct visualization. The conjunctival and Tenon's capsule incisions should not be made directly over the muscle; this will result in scarring of the muscle to Tenon's capsule or of Tenon's capsule to the muscle insertion stump, promoting restriction. The muscle should be isolated under direct visualization or inspected carefully after isolation, with great care taken not to incorporate any unnecessary Tenon's capsule in the muscle and to avoid the inferior oblique msucle on lateral rectus muscle surgery. A blind, deep sweep is completely contraindicated. The intermuscular membranes, check ligaments, and muscle foot plates must be completely severed, under direct visualization, well beyond the resection site in the course of a resection,and 5 to 8 mm. behind the insertion in the course of a recession. Complete hemostasis during the procedure and at its completion is essential; 2.5 percent phenylephrine and minimal cautery will help achieve this. Since cautery will result in a scar of Tenon's capsule to that area of the sclera, it should be used as little as possible. Phenylephrine 2.5 percent has been used safely with halothane anesthesia in many patients without significant arrhythmias. The secondary treatment of restriction is very difficult, whereas their prevention requires only good surgical technique and careful attention to detail.", "contents": "Role of Tenon's capsule in postoperative restrictions. Management of all restricted strabismus cases is difficult and frequently will require two or more procedures. The prevention of these restrictions at the time of primary surgery is far more important. All tissue should be handled as gently as possible, with smooth or fine-tip forceps, avoiding large fixation devices. Sharp dissection is essential - tissue should not be torn but cut carefully and sharply with scissors under direct visualization. The conjunctival and Tenon's capsule incisions should not be made directly over the muscle; this will result in scarring of the muscle to Tenon's capsule or of Tenon's capsule to the muscle insertion stump, promoting restriction. The muscle should be isolated under direct visualization or inspected carefully after isolation, with great care taken not to incorporate any unnecessary Tenon's capsule in the muscle and to avoid the inferior oblique msucle on lateral rectus muscle surgery. A blind, deep sweep is completely contraindicated. The intermuscular membranes, check ligaments, and muscle foot plates must be completely severed, under direct visualization, well beyond the resection site in the course of a resection,and 5 to 8 mm. behind the insertion in the course of a recession. Complete hemostasis during the procedure and at its completion is essential; 2.5 percent phenylephrine and minimal cautery will help achieve this. Since cautery will result in a scar of Tenon's capsule to that area of the sclera, it should be used as little as possible. Phenylephrine 2.5 percent has been used safely with halothane anesthesia in many patients without significant arrhythmias. The secondary treatment of restriction is very difficult, whereas their prevention requires only good surgical technique and careful attention to detail.", "PMID": 971992}
{"id": "pubmed23n0032_8776", "title": "Special considerations and techniques in strabismus surgery.", "content": "Strabismus surgery continues to present a challenge to the ophthalmologist who evaluates results critically. Precise diagnoses and expert surgical judgment never will be supplanted by tools and techniques. Nevertheless, by remaining aware of newer surgical procedures and improved instrumentation, the strabismus surgeon will continue to increase the probability of achieving desirable surgical results.", "contents": "Special considerations and techniques in strabismus surgery. Strabismus surgery continues to present a challenge to the ophthalmologist who evaluates results critically. Precise diagnoses and expert surgical judgment never will be supplanted by tools and techniques. Nevertheless, by remaining aware of newer surgical procedures and improved instrumentation, the strabismus surgeon will continue to increase the probability of achieving desirable surgical results.", "PMID": 971995}
{"id": "pubmed23n0032_8777", "title": "Surgery for congenital esotropia.", "content": "The diagnosis of esotropia in an infant is not generally a difficult one for a skilled ophthalmologist to confirm. Uncovering all the subtleties of the disease presents the greater challenge and taxes the skills involved in examining infants. The numerous treatment regimens available leave the surgeon who does this operation only occasionally in doubt as to when it is best to operate and by what method. The safest guidelines for the ophthalmologist are (1) to be sure of his diagnosis and all of its ramifications and (2) to operate on these patients by the method he feels will best totally correct both the horizontal and vertical defects in one operation. It is also known, however, that not all of these infants will be corrected by this single procedure and that additional surgery, both early and delayed, will be necessary. Similarly, amblyopia and accommodative esotropias will need to be treated in a significant segment of these patients. The stability of the final result depends upon the success of the treatment in all parameters. The results obtained are far from ideal. Some patients achieve the optimum with good and equal acuity in each eye, straight eyes, peripheral fusion, fusional amplitudes, and reduced stereopsis. Other patients will have reduced visual acuity secondary to amblyopia; deficient or no binocular cooperation; and larger eso-angles or even consecutive exo-angles - both of which will require further surgery. Congenital esotropia is a fascinating entity that requires more research before its etiology and pathogenesis will finally be understood. Only then will therapy produce a satisfactory and lasting result.", "contents": "Surgery for congenital esotropia. The diagnosis of esotropia in an infant is not generally a difficult one for a skilled ophthalmologist to confirm. Uncovering all the subtleties of the disease presents the greater challenge and taxes the skills involved in examining infants. The numerous treatment regimens available leave the surgeon who does this operation only occasionally in doubt as to when it is best to operate and by what method. The safest guidelines for the ophthalmologist are (1) to be sure of his diagnosis and all of its ramifications and (2) to operate on these patients by the method he feels will best totally correct both the horizontal and vertical defects in one operation. It is also known, however, that not all of these infants will be corrected by this single procedure and that additional surgery, both early and delayed, will be necessary. Similarly, amblyopia and accommodative esotropias will need to be treated in a significant segment of these patients. The stability of the final result depends upon the success of the treatment in all parameters. The results obtained are far from ideal. Some patients achieve the optimum with good and equal acuity in each eye, straight eyes, peripheral fusion, fusional amplitudes, and reduced stereopsis. Other patients will have reduced visual acuity secondary to amblyopia; deficient or no binocular cooperation; and larger eso-angles or even consecutive exo-angles - both of which will require further surgery. Congenital esotropia is a fascinating entity that requires more research before its etiology and pathogenesis will finally be understood. Only then will therapy produce a satisfactory and lasting result.", "PMID": 971997}
{"id": "pubmed23n0032_8778", "title": "Smooth muscle regeneration in swine ureters: a light and electron microscopic study.", "content": "Intubated ureterotomies were performed on 16 juvenile pigs. The animals were killed at intervals of from 2 to 91 days after operation and tissue sections examined with light and electron microscopes. Ureteral wall replacement occurred within the 3 months after operation and included all tissue layers. Epithelialization of the wound surfaces occurred in the 1st week and was accompanied by a connective proliferation. Smooth muscle bridged the defect within 5 weeks and the margins of the muscularis were thickened and cellular in early stages; cells were elongated and extended towards the defect from the muscular remnant. Primitive smooth muscle cells were present at defect margins. This evidence indicates that smooth muscle was replaced by hyperplasia.", "contents": "Smooth muscle regeneration in swine ureters: a light and electron microscopic study. Intubated ureterotomies were performed on 16 juvenile pigs. The animals were killed at intervals of from 2 to 91 days after operation and tissue sections examined with light and electron microscopes. Ureteral wall replacement occurred within the 3 months after operation and included all tissue layers. Epithelialization of the wound surfaces occurred in the 1st week and was accompanied by a connective proliferation. Smooth muscle bridged the defect within 5 weeks and the margins of the muscularis were thickened and cellular in early stages; cells were elongated and extended towards the defect from the muscular remnant. Primitive smooth muscle cells were present at defect margins. This evidence indicates that smooth muscle was replaced by hyperplasia.", "PMID": 972000}
{"id": "pubmed23n0032_8779", "title": "Repair of the injured renal papillary tissue in rats.", "content": "Renal papillary necrosis was produced in rats by administration of 2-bromoethylamine hydrobromide. The rats drank either 0.9 per cent sodium chloride solution or distilled water, and structural restoration of renal papilla and effect on urine-concentrating ability were examined. All rats drinking distilled water had no evidence of structural restoration of the injured papilla and no significant improvement of urine-concentrating ability. When rats started drinking 0.9 per cent sodium chloride solution shortly after indution of the necrosis, enhanced cellular reconstitution of the necrosed papillary tissue and partial restoration of urine-concentrating ability occurred in a significant number of rats. The most successful structural reparation was the collecting tubules, followed by the thin loops of Henle and the capillaries. Repopulation of the interstitial cells was the least successful. In conclusion, repair of necrosed renal papilla was enhanced when rats drank 0.9 per cent sodium chloride solution starting shortly after injection of renal papilla-necrotizing dose of 2-bromoethylamine hydrobromide.", "contents": "Repair of the injured renal papillary tissue in rats. Renal papillary necrosis was produced in rats by administration of 2-bromoethylamine hydrobromide. The rats drank either 0.9 per cent sodium chloride solution or distilled water, and structural restoration of renal papilla and effect on urine-concentrating ability were examined. All rats drinking distilled water had no evidence of structural restoration of the injured papilla and no significant improvement of urine-concentrating ability. When rats started drinking 0.9 per cent sodium chloride solution shortly after indution of the necrosis, enhanced cellular reconstitution of the necrosed papillary tissue and partial restoration of urine-concentrating ability occurred in a significant number of rats. The most successful structural reparation was the collecting tubules, followed by the thin loops of Henle and the capillaries. Repopulation of the interstitial cells was the least successful. In conclusion, repair of necrosed renal papilla was enhanced when rats drank 0.9 per cent sodium chloride solution starting shortly after injection of renal papilla-necrotizing dose of 2-bromoethylamine hydrobromide.", "PMID": 972001}
{"id": "pubmed23n0032_8780", "title": "The epitaxially induced crystal growth of calcium oxalate by crystalline uric acid.", "content": "Seed crystals of anhydrous uric acid were shown to epitaxially induce the crystal growth of calcium oxalate monohydrate from its metastable supersaturated solution. Scanning electron microscopic examination of crystalline material isolated from the kinetic experiments supports the chemical evidence that calcium oxalate had been nucleated by uric acid. In spite of close lattice similarities between some of the faces of the two phases, the induction period required for heterogeneous nucleation by the seed material was about half of that required for precipitation in the absence of potential nucleating agents. This has created some doubt as to whether crystalline uric acid has an influence on the formation of calcium oxalate lithiasis.", "contents": "The epitaxially induced crystal growth of calcium oxalate by crystalline uric acid. Seed crystals of anhydrous uric acid were shown to epitaxially induce the crystal growth of calcium oxalate monohydrate from its metastable supersaturated solution. Scanning electron microscopic examination of crystalline material isolated from the kinetic experiments supports the chemical evidence that calcium oxalate had been nucleated by uric acid. In spite of close lattice similarities between some of the faces of the two phases, the induction period required for heterogeneous nucleation by the seed material was about half of that required for precipitation in the absence of potential nucleating agents. This has created some doubt as to whether crystalline uric acid has an influence on the formation of calcium oxalate lithiasis.", "PMID": 972002}
{"id": "pubmed23n0032_8781", "title": "Comparative effects of anticalculus management in the rat.", "content": "Utilizing a low pyridoxine, ethylene glycol-fed rat model, various modes of anticalculus therapy were compared. Prophylactic papillary and parenchymal oxalate stone therapy is most effective with magnesium oxide. Hydrochlorothiazide is next in order as the therapy of choice in this group. A high calcium diet seems to negate the effect of magnesium oxide. Methylene blue, magnesium oxide, and even sodium phosphate, are not as effective as hydrochlorothiazide in the combined prevention and treatment of preformed parencymal calcium oxalate stones. No agent seems to have a profound effect on preformed apical papillary calculi.", "contents": "Comparative effects of anticalculus management in the rat. Utilizing a low pyridoxine, ethylene glycol-fed rat model, various modes of anticalculus therapy were compared. Prophylactic papillary and parenchymal oxalate stone therapy is most effective with magnesium oxide. Hydrochlorothiazide is next in order as the therapy of choice in this group. A high calcium diet seems to negate the effect of magnesium oxide. Methylene blue, magnesium oxide, and even sodium phosphate, are not as effective as hydrochlorothiazide in the combined prevention and treatment of preformed parencymal calcium oxalate stones. No agent seems to have a profound effect on preformed apical papillary calculi.", "PMID": 972003}
{"id": "pubmed23n0032_8782", "title": "Effect of tetracycline on calcium oxalate calculi: in vivo and in vitro studies.", "content": "Inadvertent use of tetracycline hydrochloride while producing calcium oxalate stones in the rat resulted in a reduction of papillary concretions by one-half. Parenchymal calcifications in the continuing long term, preformed stone group were reduced to levels comparable to that of other useful agents. In vitro studies corroborated these findings at physiologic urinary pH ranges. The rationale and comparisons to other anticalculus drugs are discussed.", "contents": "Effect of tetracycline on calcium oxalate calculi: in vivo and in vitro studies. Inadvertent use of tetracycline hydrochloride while producing calcium oxalate stones in the rat resulted in a reduction of papillary concretions by one-half. Parenchymal calcifications in the continuing long term, preformed stone group were reduced to levels comparable to that of other useful agents. In vitro studies corroborated these findings at physiologic urinary pH ranges. The rationale and comparisons to other anticalculus drugs are discussed.", "PMID": 972004}
{"id": "pubmed23n0032_8783", "title": "The absorption metabolism, and excretion of Estracyt (NSC 89199) in patients with prostatic cancer.", "content": "Estraumustine phosphate (estradiol, 3-N-[bis(2-chloroethyl)-] carbamate-17beta-dihydrogenphosphate (Estracyt) labeled with 3H in the estradiol moiety and 14C in the carbamate moiety was synthesized, and its absorption, metabolism, and excretion were studied after oral administration to three patients with prostatic carcinoma. One of the patients was also given the same dose by intravenous injection. In addition to monitoring isotope levels in peripheral blood, urine, and feces, samples of portal vein blood were obtained through a catheter in the umbilical vein. Analyses of portal blood samples revealed that most of the estramustine phosphate was dephosphorylated to estramustine during absorption. Estramustine was found to be the major metabolite in peripheral blood after oral as well as after intravenous administration. The urinary excretion data appeared to warrent the conclusion that most of the carbamate ester of estraumustine is hydrolyzed before it is excreted. In the patient given estramustine phosphate by both routes the absorption of the compound when given by mouth was found to be approximately 75 per cent.", "contents": "The absorption metabolism, and excretion of Estracyt (NSC 89199) in patients with prostatic cancer. Estraumustine phosphate (estradiol, 3-N-[bis(2-chloroethyl)-] carbamate-17beta-dihydrogenphosphate (Estracyt) labeled with 3H in the estradiol moiety and 14C in the carbamate moiety was synthesized, and its absorption, metabolism, and excretion were studied after oral administration to three patients with prostatic carcinoma. One of the patients was also given the same dose by intravenous injection. In addition to monitoring isotope levels in peripheral blood, urine, and feces, samples of portal vein blood were obtained through a catheter in the umbilical vein. Analyses of portal blood samples revealed that most of the estramustine phosphate was dephosphorylated to estramustine during absorption. Estramustine was found to be the major metabolite in peripheral blood after oral as well as after intravenous administration. The urinary excretion data appeared to warrent the conclusion that most of the carbamate ester of estraumustine is hydrolyzed before it is excreted. In the patient given estramustine phosphate by both routes the absorption of the compound when given by mouth was found to be approximately 75 per cent.", "PMID": 972005}
{"id": "pubmed23n0032_8784", "title": "Substrate metabolism in the isolated perfused dog kidney.", "content": "The relationships between substrate utilization and renal work were investigated in an isolated, perfused dog kidney preparation: substrate utilization included free-fatty acid (FFA), glucose, and oxygen, whereas work was assessed by measuring TNa+. The magnitude of TNa+ was varied by altering glomerular filtration rate as a consequence of changing renal blood flow. As the rate of renal blood flow was reduced, both Qo2, and QFFA decreased while Qglucose increased. The reduction in QFFA and Qo2 paralleled the decrease in TNa+. An inverse relationship was noted between QFFA and Qglucose in all experiments. The results suggest that FFA and perhaps glucose may serve as a significant substrate for providing energy for TNA+ in the kidney. Further, since QFFA and Qglucose are inversely related to each other, glucose may substitute for FFA as an energy source at times when QFFA is reduced.", "contents": "Substrate metabolism in the isolated perfused dog kidney. The relationships between substrate utilization and renal work were investigated in an isolated, perfused dog kidney preparation: substrate utilization included free-fatty acid (FFA), glucose, and oxygen, whereas work was assessed by measuring TNa+. The magnitude of TNa+ was varied by altering glomerular filtration rate as a consequence of changing renal blood flow. As the rate of renal blood flow was reduced, both Qo2, and QFFA decreased while Qglucose increased. The reduction in QFFA and Qo2 paralleled the decrease in TNa+. An inverse relationship was noted between QFFA and Qglucose in all experiments. The results suggest that FFA and perhaps glucose may serve as a significant substrate for providing energy for TNA+ in the kidney. Further, since QFFA and Qglucose are inversely related to each other, glucose may substitute for FFA as an energy source at times when QFFA is reduced.", "PMID": 972006}
{"id": "pubmed23n0032_8785", "title": "An experimental study of the calcification and absorption of polyglycolic acid and catgut sutures within the urinary tract.", "content": "This experimental study has shown that there is no difference in calcification on catgut and polyglycolic acid suture loops within the sterile and infected rat bladder. The presence of an experimentally induced infection reduces the time both catgut and polyglycolic acid sutures remain attached to the bladder wall. Polyglycolic acid sutures can nevertheless be recommended for routine use in the urinary tract whenever an absorbable suture is required in view of its greater strength, reliability and remarkable lack of inflammatory tissue reaction.", "contents": "An experimental study of the calcification and absorption of polyglycolic acid and catgut sutures within the urinary tract. This experimental study has shown that there is no difference in calcification on catgut and polyglycolic acid suture loops within the sterile and infected rat bladder. The presence of an experimentally induced infection reduces the time both catgut and polyglycolic acid sutures remain attached to the bladder wall. Polyglycolic acid sutures can nevertheless be recommended for routine use in the urinary tract whenever an absorbable suture is required in view of its greater strength, reliability and remarkable lack of inflammatory tissue reaction.", "PMID": 972007}
{"id": "pubmed23n0032_8786", "title": "Role of neurogenic factors in canine urethral wall tension and urinary continence.", "content": "A plastic disc, secured submucosally, was inserted in the canine bladder to permit filling of the bladder neck region while bypassing the accommodating ability of the detrusor. The bladder neck region was distended with radiopaque solution and the onset of flow in the urethra and the width of the urethra at steady state flow conditions were monitored by fluoroscopy. Perfusion pressure was also recorded. Bilateral section of the hypogastric nerves produced a substantial reduction in the time to initial flow and an increase in the width of the urethra with a decrease in pressure at steady state flow. Bilateral section of the pelvic nerves or administration of atropine reduced urethral wall tension at steady state flow but did not significantly influence the onset of initial flow. Gallamine treatment had no significant effect either on wall tension or on the onset of initial flow. It was estimated that the sympathetic nervous system contributed about 30 per cent to the urethral wall tension at steady state flow conditions whereas the parasympathetic nervous system contributed about 10 per cent. The remaining 60 per cent was attributed to the elastic properties of the urethra. In the maintenance of urinary continence, the sympathetic nervous system was estimated to contribute almost 60 per cent based on our experimental system. No other neurogenic influence was found to contribute significantly. Elastic and mechanical (anatomic) factors were probably responsible for the remaining 40 per cent. It was concluded that the sympathetic nervous system has a significant role in the maintenance of urinary continence.", "contents": "Role of neurogenic factors in canine urethral wall tension and urinary continence. A plastic disc, secured submucosally, was inserted in the canine bladder to permit filling of the bladder neck region while bypassing the accommodating ability of the detrusor. The bladder neck region was distended with radiopaque solution and the onset of flow in the urethra and the width of the urethra at steady state flow conditions were monitored by fluoroscopy. Perfusion pressure was also recorded. Bilateral section of the hypogastric nerves produced a substantial reduction in the time to initial flow and an increase in the width of the urethra with a decrease in pressure at steady state flow. Bilateral section of the pelvic nerves or administration of atropine reduced urethral wall tension at steady state flow but did not significantly influence the onset of initial flow. Gallamine treatment had no significant effect either on wall tension or on the onset of initial flow. It was estimated that the sympathetic nervous system contributed about 30 per cent to the urethral wall tension at steady state flow conditions whereas the parasympathetic nervous system contributed about 10 per cent. The remaining 60 per cent was attributed to the elastic properties of the urethra. In the maintenance of urinary continence, the sympathetic nervous system was estimated to contribute almost 60 per cent based on our experimental system. No other neurogenic influence was found to contribute significantly. Elastic and mechanical (anatomic) factors were probably responsible for the remaining 40 per cent. It was concluded that the sympathetic nervous system has a significant role in the maintenance of urinary continence.", "PMID": 972008}
{"id": "pubmed23n0032_8787", "title": "Comparison of rapid versus slow decompression of the distended urinary bladder.", "content": "Experiments are described showing that urinary retention with its associated increased intravesical pressure and overstretching of the bladder wall can lead to loss of capillary and tissue integrity. Our findings indicate that the severity of damage to the bladder wall prior to catheterization determines the degree of hemorrhage after bladder decompression and not the rate of emptying.", "contents": "Comparison of rapid versus slow decompression of the distended urinary bladder. Experiments are described showing that urinary retention with its associated increased intravesical pressure and overstretching of the bladder wall can lead to loss of capillary and tissue integrity. Our findings indicate that the severity of damage to the bladder wall prior to catheterization determines the degree of hemorrhage after bladder decompression and not the rate of emptying.", "PMID": 972009}
{"id": "pubmed23n0032_8788", "title": "Total replacement of part of the canine urethra with lyophilized vein homografts.", "content": "Three centimeters of the urethra were replaced by lyophilized homograft veins in nine male dogs. The dogs were followed for up to 6 months with periodic urethrograms. The grafted lyophilized veins served as a guide for the ingrowing transitional cell epithelium. The graft caused very little tissue reaction and hardly any formation of fibrous tissue. The urethra was covered by transitional cell epithelium after 4 to 8 weeks, and eight of the nine dogs showed no signs of obstruction during the follow-up period.", "contents": "Total replacement of part of the canine urethra with lyophilized vein homografts. Three centimeters of the urethra were replaced by lyophilized homograft veins in nine male dogs. The dogs were followed for up to 6 months with periodic urethrograms. The grafted lyophilized veins served as a guide for the ingrowing transitional cell epithelium. The graft caused very little tissue reaction and hardly any formation of fibrous tissue. The urethra was covered by transitional cell epithelium after 4 to 8 weeks, and eight of the nine dogs showed no signs of obstruction during the follow-up period.", "PMID": 972010}
{"id": "pubmed23n0032_8789", "title": "Incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate after in vivo treatment with estradiol-3N-BIS(2-chloroethyl)carbamate-17 beta-phosphate (Estracyt) and its estrogen and cytostatic parts.", "content": "The in vivo effect of Estracyt (a nitrogen mustard linked to estradiol-17 beta-phosphate) on testosterone-stimulated incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate of castrated rats has been investigated. A dose of 100 mg of Estracyt per kg of body weight injected intravenously daily for 3 days significantly reduced isotope incorporation. Equivalent amounts of the two main components of Estracyt, the nitrogen mustard and estradiol-17 beta-phosphate, were not able to mimic this Estracyt effect. Possible implications of this difference and the intracellular mechanism of action of Estracyt are discussed.", "contents": "Incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate after in vivo treatment with estradiol-3N-BIS(2-chloroethyl)carbamate-17 beta-phosphate (Estracyt) and its estrogen and cytostatic parts. The in vivo effect of Estracyt (a nitrogen mustard linked to estradiol-17 beta-phosphate) on testosterone-stimulated incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate of castrated rats has been investigated. A dose of 100 mg of Estracyt per kg of body weight injected intravenously daily for 3 days significantly reduced isotope incorporation. Equivalent amounts of the two main components of Estracyt, the nitrogen mustard and estradiol-17 beta-phosphate, were not able to mimic this Estracyt effect. Possible implications of this difference and the intracellular mechanism of action of Estracyt are discussed.", "PMID": 972011}
{"id": "pubmed23n0032_8790", "title": "The influence of hyperbaric oxygenation on rat testes.", "content": "Using Wistar stain male rats, it has been shown that 2 atmospheric pressures associated with oxygen saturation, together or singly for 90 minutes daily for successive 46 days, resulted in no significant change on the ratio of testes to body weight. No significant change was observed in the incorporation of 3H-thymidine into testes and pituitary glands between the pressure-treated groups and the control group.", "contents": "The influence of hyperbaric oxygenation on rat testes. Using Wistar stain male rats, it has been shown that 2 atmospheric pressures associated with oxygen saturation, together or singly for 90 minutes daily for successive 46 days, resulted in no significant change on the ratio of testes to body weight. No significant change was observed in the incorporation of 3H-thymidine into testes and pituitary glands between the pressure-treated groups and the control group.", "PMID": 972012}
{"id": "pubmed23n0032_8791", "title": "Osteoporosis and fractures of the neck of the femur: some epidemiologic considerations.", "content": "The prevalence of osteoporosis in Jerusalem during the years 1967-71 was studied in a general population sample and in patients with fractures of the neck of the femur. Osteoporosis was determined by qualitative examination of lateral X-ray films of the lumbar spine. The prevalence of definite osteoporosis in a random population sample was 8.6%, and was higher in females (14.5%) than in males (2.9%). The prevalence of definite osteoporosis among 389 patients with fractures of the neck of the femur was 32.2%--37.8% in females and 17.4% in males. The results of this survey support the conclusion that osteoporosis plays an important role in the pathogenesis of fractures of the neck of the femur.", "contents": "Osteoporosis and fractures of the neck of the femur: some epidemiologic considerations. The prevalence of osteoporosis in Jerusalem during the years 1967-71 was studied in a general population sample and in patients with fractures of the neck of the femur. Osteoporosis was determined by qualitative examination of lateral X-ray films of the lumbar spine. The prevalence of definite osteoporosis in a random population sample was 8.6%, and was higher in females (14.5%) than in males (2.9%). The prevalence of definite osteoporosis among 389 patients with fractures of the neck of the femur was 32.2%--37.8% in females and 17.4% in males. The results of this survey support the conclusion that osteoporosis plays an important role in the pathogenesis of fractures of the neck of the femur.", "PMID": 972013}
{"id": "pubmed23n0032_8792", "title": "Menopausal bone loss and estrogen replacement.", "content": "Throughout adult life the bone mineral mass of the radius is greater in males than in females. In males, it decreases after 60 years of age, while in females, it decreases earlier, at approximately 50 years, and the loss is greater. At the average age of 67 years, one half of the normal white female population has less than the normal amount of bone in the radius. Premenopausal women over the age of 50 do not show any decline of bone mineral mass, while in postmenopausal women, regardless of age, there is a loss of bone mass related to the number of years after menopause. Castrated women have significantly less bone mass than premenopausal women of the same average age. No decrease in cortical thickness of the radius was found in oophorectomized women treated with estrogens after castration. In a long-term, follow-up study, untreated postmenopausal women (after a natural or an artifical menopause) showed a significant loss of bone mass, while estrogen-treated, postmenopausal women showed no such loss. Estrogen treatment thus appears to prevent postmenopausal bone loss.", "contents": "Menopausal bone loss and estrogen replacement. Throughout adult life the bone mineral mass of the radius is greater in males than in females. In males, it decreases after 60 years of age, while in females, it decreases earlier, at approximately 50 years, and the loss is greater. At the average age of 67 years, one half of the normal white female population has less than the normal amount of bone in the radius. Premenopausal women over the age of 50 do not show any decline of bone mineral mass, while in postmenopausal women, regardless of age, there is a loss of bone mass related to the number of years after menopause. Castrated women have significantly less bone mass than premenopausal women of the same average age. No decrease in cortical thickness of the radius was found in oophorectomized women treated with estrogens after castration. In a long-term, follow-up study, untreated postmenopausal women (after a natural or an artifical menopause) showed a significant loss of bone mass, while estrogen-treated, postmenopausal women showed no such loss. Estrogen treatment thus appears to prevent postmenopausal bone loss.", "PMID": 972014}
{"id": "pubmed23n0032_8793", "title": "Prevention of bone loss following oophorectomy in premenopausal women: a retrospective assessment of the effects of oophorectomy and a prospective controlled trial of the effects of mestranol therapy.", "content": "Prospective studies of bone mass in women following oophorectomy for benign conditions were done by the double-blind technique. Skeletal response to treatment was measured by photon absorption densitometry. Untreated patients were found to lose bone mass rapidly during the first two years after oophorectomy. When estrogen replacement was started within two months of oophorectomy, it was found to be effective in preventing subsequent bone tissue loss. Three years following oophorectomy, untreated women who had already lost bone tissue, and who were then started on estrogen replacement, showed a highly significant increase in their bone mass. The women in whom this treatment was delayed for six years did not respond. No untoward effects were noted in these women, perhaps, in part, because they had undergone hysterectomy. Long-term effects of this treatment are now being evaluated.", "contents": "Prevention of bone loss following oophorectomy in premenopausal women: a retrospective assessment of the effects of oophorectomy and a prospective controlled trial of the effects of mestranol therapy. Prospective studies of bone mass in women following oophorectomy for benign conditions were done by the double-blind technique. Skeletal response to treatment was measured by photon absorption densitometry. Untreated patients were found to lose bone mass rapidly during the first two years after oophorectomy. When estrogen replacement was started within two months of oophorectomy, it was found to be effective in preventing subsequent bone tissue loss. Three years following oophorectomy, untreated women who had already lost bone tissue, and who were then started on estrogen replacement, showed a highly significant increase in their bone mass. The women in whom this treatment was delayed for six years did not respond. No untoward effects were noted in these women, perhaps, in part, because they had undergone hysterectomy. Long-term effects of this treatment are now being evaluated.", "PMID": 972015}
{"id": "pubmed23n0032_8794", "title": "Role of hormonal factors in the pathogenesis of postmenopausal osteoporosis.", "content": "In 47 women with postmenopausal osteoporosis, pretreatment studies by microradiography, radioimmunoassay and other methods showed increased bone resorption, normal bone formation, and decreased serum immunoreactive parathyroid hormone (PTH). In patients treated with a physiologic replacement dose of estrogen, bone resorption decreased to normal and PTH increased after short-term therapy; bone formation decreased to very low levels after long-term therapy. These data indicate that, in most patients, both an intrinsic abnormality of bone cell function and a disruption of the normal regulation of bone turnover by PTH and sex hormones, as a result of the menopause, are important in the pathogenesis of osteoporosis.", "contents": "Role of hormonal factors in the pathogenesis of postmenopausal osteoporosis. In 47 women with postmenopausal osteoporosis, pretreatment studies by microradiography, radioimmunoassay and other methods showed increased bone resorption, normal bone formation, and decreased serum immunoreactive parathyroid hormone (PTH). In patients treated with a physiologic replacement dose of estrogen, bone resorption decreased to normal and PTH increased after short-term therapy; bone formation decreased to very low levels after long-term therapy. These data indicate that, in most patients, both an intrinsic abnormality of bone cell function and a disruption of the normal regulation of bone turnover by PTH and sex hormones, as a result of the menopause, are important in the pathogenesis of osteoporosis.", "PMID": 972016}
{"id": "pubmed23n0032_8795", "title": "Relation to osteoporosis of age- and hormone-induced changes in the metabolism of collagen and bone.", "content": "Experimental evidence is presented which suggests that age-induced changes in the collagenous matrix, the main constituent of the organic portion of bones, are at least partially responsible for age-induced physiological osteoporotic changes in the skeleton. In particular, there seems to be a labile fraction of recently synthesized collagen in bones, which loses its metabolic activity rapidly with advancing age. Experimental and clinical hormonal disorders and disturbances in calcium metabolism also cause changes in skeletal metabolism; these changes seem to be largely mediated through changes in the collagenous matrix. In experimental hyperthyroidism and hyperparathyroidism, the rate of degradation of the collagenous matrix appears to act as a moderator or \"final messenger\" in hormone-induced bone resorption. In conditions with altered calcium metabolism, such as malabsorption associated with hypocalcemia, altered bone metabolism may be due to osteomalacia or hypocalcemia-induced hyperparathyroidism. An increase in the rate of bone destruction in relation to the rate of bone formation is probably also the cause of postmenopausal osteoporosis. At present there is no optimal form of hormonal treatment for age-induced or post menopausal osteoporosis. Estrogen replacement therapy may be the best available treatment for postmenopausal osteoporosis, but slowing down the already low rate of bone catabolism in elderly subjects by estrogen or other therapeutic means requires long periods of treatment before pronounced increases in the total mass of bones take place and prophylactic administration of estrogen may produce better results.", "contents": "Relation to osteoporosis of age- and hormone-induced changes in the metabolism of collagen and bone. Experimental evidence is presented which suggests that age-induced changes in the collagenous matrix, the main constituent of the organic portion of bones, are at least partially responsible for age-induced physiological osteoporotic changes in the skeleton. In particular, there seems to be a labile fraction of recently synthesized collagen in bones, which loses its metabolic activity rapidly with advancing age. Experimental and clinical hormonal disorders and disturbances in calcium metabolism also cause changes in skeletal metabolism; these changes seem to be largely mediated through changes in the collagenous matrix. In experimental hyperthyroidism and hyperparathyroidism, the rate of degradation of the collagenous matrix appears to act as a moderator or \"final messenger\" in hormone-induced bone resorption. In conditions with altered calcium metabolism, such as malabsorption associated with hypocalcemia, altered bone metabolism may be due to osteomalacia or hypocalcemia-induced hyperparathyroidism. An increase in the rate of bone destruction in relation to the rate of bone formation is probably also the cause of postmenopausal osteoporosis. At present there is no optimal form of hormonal treatment for age-induced or post menopausal osteoporosis. Estrogen replacement therapy may be the best available treatment for postmenopausal osteoporosis, but slowing down the already low rate of bone catabolism in elderly subjects by estrogen or other therapeutic means requires long periods of treatment before pronounced increases in the total mass of bones take place and prophylactic administration of estrogen may produce better results.", "PMID": 972017}
{"id": "pubmed23n0032_8796", "title": "Effect of conventional and newer forms of treatment on calcium absorption in osteoporosis.", "content": "Studies carried out in this Research Unit and by others have shown that patients with osteoporosis absorb less calcium from a high calcium intake than do patients without osteoporosis. To determine whether various treatment modalities would increase the intestinal absorption of calcium, the effects of conventional and new methods were investigated. Tracer doses of 47Ca were given orally to determine the absorption of calcium. Calcium balances were also determined. Estrogen decreased urinary calcium and testosterone increased the nitrogen balance. Vitamin D and strontium increased the urinary calcium but did not increase calcium absorption. Of the newer compounds, fluoride did not affect calcium absorption but decreased urinary calcium. Phosphate had a similar effect. Infusions containing up to 15 mg calcium/kg, given for 6 or 10 days, increased the retention of calcium but did not increase its absorption.", "contents": "Effect of conventional and newer forms of treatment on calcium absorption in osteoporosis. Studies carried out in this Research Unit and by others have shown that patients with osteoporosis absorb less calcium from a high calcium intake than do patients without osteoporosis. To determine whether various treatment modalities would increase the intestinal absorption of calcium, the effects of conventional and new methods were investigated. Tracer doses of 47Ca were given orally to determine the absorption of calcium. Calcium balances were also determined. Estrogen decreased urinary calcium and testosterone increased the nitrogen balance. Vitamin D and strontium increased the urinary calcium but did not increase calcium absorption. Of the newer compounds, fluoride did not affect calcium absorption but decreased urinary calcium. Phosphate had a similar effect. Infusions containing up to 15 mg calcium/kg, given for 6 or 10 days, increased the retention of calcium but did not increase its absorption.", "PMID": 972018}
{"id": "pubmed23n0032_8797", "title": "Rehabilitation in postmenopausal osteoporosis.", "content": "Rehabilitation and nursing care of patients with postmenopausal osteoporosis require a comprehensive team effort. The primary purpose of rehabilitation is to restore these elderly women to an optimal level of well-being and activity and return them to home and community life as soon as possible. Medical management, rehabilitative nursing and physical therapy are interdependent. Emphasis is placed on estrogen replacement to prevent further fractures and height loss, individualization of care, early ambulation, improving patient confidence, physiotherapy procedures, orthopedic supports, optimal nutrition, safety precautions, work simplification, occupational therapy and the need for appropriate social contacts. The economic, physical and emotional consequences of deforming postmenopausal osteoporosis are enormous. Current research indicates that this disease is preventible. An effective program of prophylaxis is needed now; otherwise many more women will need increasing amounts of costly rehabilitation.", "contents": "Rehabilitation in postmenopausal osteoporosis. Rehabilitation and nursing care of patients with postmenopausal osteoporosis require a comprehensive team effort. The primary purpose of rehabilitation is to restore these elderly women to an optimal level of well-being and activity and return them to home and community life as soon as possible. Medical management, rehabilitative nursing and physical therapy are interdependent. Emphasis is placed on estrogen replacement to prevent further fractures and height loss, individualization of care, early ambulation, improving patient confidence, physiotherapy procedures, orthopedic supports, optimal nutrition, safety precautions, work simplification, occupational therapy and the need for appropriate social contacts. The economic, physical and emotional consequences of deforming postmenopausal osteoporosis are enormous. Current research indicates that this disease is preventible. An effective program of prophylaxis is needed now; otherwise many more women will need increasing amounts of costly rehabilitation.", "PMID": 972019}
{"id": "pubmed23n0032_8798", "title": "Unsaturated fatty acids in adipose tissue of Israeli Jews.", "content": "The ratio of polyunsaturated to saturated fatty acids in the adipose tissue of Israelis was found to be 0.88 for Ashkenazic Jews and 1.13 for non-Ashkenazic Jews, the highest reported for any population on a free-choice diet. Despite the unusually high ratios, indicating a high consumption of polyunsaturated fat, there is nevertheless, a relatively high incidence of ischemic heart disease among Israelis.", "contents": "Unsaturated fatty acids in adipose tissue of Israeli Jews. The ratio of polyunsaturated to saturated fatty acids in the adipose tissue of Israelis was found to be 0.88 for Ashkenazic Jews and 1.13 for non-Ashkenazic Jews, the highest reported for any population on a free-choice diet. Despite the unusually high ratios, indicating a high consumption of polyunsaturated fat, there is nevertheless, a relatively high incidence of ischemic heart disease among Israelis.", "PMID": 972020}
{"id": "pubmed23n0032_8799", "title": "Morphological and cytochemical investigation of human mesothelial cells from pleural and peritoneal effusions: a light and electron microscopy study.", "content": "We investigated the morphological and cytochemical properties of mesothelial cells obtained from pleural and peritoneal effusions of patients with various diseases. The morphological features were studied by light and electron microscopy and by cytochemical methods. The different kinds of contact between mesothelial cells and their interaction with lymphocytes were observed and the transition of mesothelial cells to macrophages and/or fibroblasts was demonstrated.", "contents": "Morphological and cytochemical investigation of human mesothelial cells from pleural and peritoneal effusions: a light and electron microscopy study. We investigated the morphological and cytochemical properties of mesothelial cells obtained from pleural and peritoneal effusions of patients with various diseases. The morphological features were studied by light and electron microscopy and by cytochemical methods. The different kinds of contact between mesothelial cells and their interaction with lymphocytes were observed and the transition of mesothelial cells to macrophages and/or fibroblasts was demonstrated.", "PMID": 972021}
{"id": "pubmed23n0032_8800", "title": "Apparent total alpha1-antitrypsin deficiency: report of a case.", "content": "A 29-year-old female, with chronic renal failure and chronic bilateral emphysema, was admitted with severe uremia and septicemia secondary to multiple abscesses in the right kidney. Her condition improved after right nephrectomy. Pulmonary function studies showed marked obstructive and restrictive lung disease consistent witht the diagnosis of primary emphysema. On biochemical and histological examination, the liver was found to be normal. Alpha1-antitrypsin could not be demonstrated in the patient's serum at normal pH by any of the known techniques, but protein molecules with alpha1-antitrypsin antigencity were found at pH 4.8; this suggests a pH-dependent structural difference in alpha1-antitrypsin protein. Starch gel electrophoresis gave a multibanding pattern not previously described. A new form of apparent total alpha-1-antitrypsin deficiency is postulated.", "contents": "Apparent total alpha1-antitrypsin deficiency: report of a case. A 29-year-old female, with chronic renal failure and chronic bilateral emphysema, was admitted with severe uremia and septicemia secondary to multiple abscesses in the right kidney. Her condition improved after right nephrectomy. Pulmonary function studies showed marked obstructive and restrictive lung disease consistent witht the diagnosis of primary emphysema. On biochemical and histological examination, the liver was found to be normal. Alpha1-antitrypsin could not be demonstrated in the patient's serum at normal pH by any of the known techniques, but protein molecules with alpha1-antitrypsin antigencity were found at pH 4.8; this suggests a pH-dependent structural difference in alpha1-antitrypsin protein. Starch gel electrophoresis gave a multibanding pattern not previously described. A new form of apparent total alpha-1-antitrypsin deficiency is postulated.", "PMID": 972022}
{"id": "pubmed23n0032_8801", "title": "Tetrazolium salts: a consumer's guide.", "content": "The purities of seven tetrazolium salts, obtained from various commercial sources, have been assessed by thin layer chromatography, relative extinction coefficients, and melting points. MTT and INT were largely homogeneous on thin layer chromatography, although significant variations occurred in the melting point behaviour. All the samples of TT examined were contaminated to a small extent with non-tetrazolium u.v.-absorbing material. TNBT and NBT were contaminated with small amounts of mono-tetrazolium salts, although one sample of each was heavily contaminated with another di-tetrazolium compound. Four samples of TNBT contained high melting point contaminants. BT was also contaminated with mono-tetrazolium salts, and some samples also contained di-tetrazolium salt contaminants. NT was the most heavily contaminated of all, most samples containing no less than five separate tetrazolium compounds. Prices varied widely, and in general were not related to purity. Some catalogue entries were very easy to find; others were more difficult. Few specifications were given; of these, most were arbitrary (for example, pure, grade I, and ... probably the finest INT offered anywhere.", "contents": "Tetrazolium salts: a consumer's guide. The purities of seven tetrazolium salts, obtained from various commercial sources, have been assessed by thin layer chromatography, relative extinction coefficients, and melting points. MTT and INT were largely homogeneous on thin layer chromatography, although significant variations occurred in the melting point behaviour. All the samples of TT examined were contaminated to a small extent with non-tetrazolium u.v.-absorbing material. TNBT and NBT were contaminated with small amounts of mono-tetrazolium salts, although one sample of each was heavily contaminated with another di-tetrazolium compound. Four samples of TNBT contained high melting point contaminants. BT was also contaminated with mono-tetrazolium salts, and some samples also contained di-tetrazolium salt contaminants. NT was the most heavily contaminated of all, most samples containing no less than five separate tetrazolium compounds. Prices varied widely, and in general were not related to purity. Some catalogue entries were very easy to find; others were more difficult. Few specifications were given; of these, most were arbitrary (for example, pure, grade I, and ... probably the finest INT offered anywhere.", "PMID": 972069}
{"id": "pubmed23n0032_8802", "title": "The composition of Erythrosins, Fluorescein, Phloxine and Rose Bengal: a study using thin-layer chromatography and solvent extraction.", "content": "Commercial samples of Erythrosin B (CI 45430), Erythrosin Y (CI 45425), Fluorescein (CI 45350), Phloxine (CI 45410) and Rose Bengal (CI 45440) have been analysed by thin-layer chromatography. The Erythrosins were found to be mixtures consisting in the main of 4'-iodofluorescein, 4', 5'-di-iodofluorescein, 2', 4', 5'-tri-iodofluorescein and 2',4',5',7'-tetraiodofluorescein, in some instances together with 2',4',5'-tri-iodo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetraiodo- 4,5,6,7-tetrachlorofluorescein. Samples of Fluorescein were mixtures of the nominal dye usually with traces of several unidentified, fluorescent components. Those of Phloxine consisted mainly of mixtures of 4'-bromo-4,5,6,7-tetrachlorofluorescein, 4',5'-dibromo-4,5,6,7'-tetrachlorofluorescein, 2',4',5'-tribromo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetrabromo-4,5,6,7-tetrachlorofluorescein, often with 4,5,6,7-tetrachlorofluorescein. Samples of Rose Bengal were mixtures of 4'-iodo-4,5,6,7-tetrachlorofluorescein,4',5'-di-iodo-4,5,6,7-tetrachlorofluorescein, 2',4',5'-tri-iodo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetraiodo-4,5,6,7-tetrachlorofluorescein together with some unidentified components. Most of the commercial dye samples gave an insoluble residue when extracted with methanol. This residue was usually inorganic carbonate or halide. Some possible practical consequences of the various impurities are discussed.", "contents": "The composition of Erythrosins, Fluorescein, Phloxine and Rose Bengal: a study using thin-layer chromatography and solvent extraction. Commercial samples of Erythrosin B (CI 45430), Erythrosin Y (CI 45425), Fluorescein (CI 45350), Phloxine (CI 45410) and Rose Bengal (CI 45440) have been analysed by thin-layer chromatography. The Erythrosins were found to be mixtures consisting in the main of 4'-iodofluorescein, 4', 5'-di-iodofluorescein, 2', 4', 5'-tri-iodofluorescein and 2',4',5',7'-tetraiodofluorescein, in some instances together with 2',4',5'-tri-iodo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetraiodo- 4,5,6,7-tetrachlorofluorescein. Samples of Fluorescein were mixtures of the nominal dye usually with traces of several unidentified, fluorescent components. Those of Phloxine consisted mainly of mixtures of 4'-bromo-4,5,6,7-tetrachlorofluorescein, 4',5'-dibromo-4,5,6,7'-tetrachlorofluorescein, 2',4',5'-tribromo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetrabromo-4,5,6,7-tetrachlorofluorescein, often with 4,5,6,7-tetrachlorofluorescein. Samples of Rose Bengal were mixtures of 4'-iodo-4,5,6,7-tetrachlorofluorescein,4',5'-di-iodo-4,5,6,7-tetrachlorofluorescein, 2',4',5'-tri-iodo-4,5,6,7-tetrachlorofluorescein and 2',4',5',7'-tetraiodo-4,5,6,7-tetrachlorofluorescein together with some unidentified components. Most of the commercial dye samples gave an insoluble residue when extracted with methanol. This residue was usually inorganic carbonate or halide. Some possible practical consequences of the various impurities are discussed.", "PMID": 972070}
{"id": "pubmed23n0032_8803", "title": "The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt.", "content": "This article describes the use of a microdensitometer for the measurement of BPST formazan in tissue sections. BPST is a new tetrazolium salt, 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride, which produces a single, well-defined formazan, and is thus easily quantified. The formazan gives an excellent localization, since BPST was originally designed for ultrastructural work. Activities are expressed in absolute units as n moles hydrogen/mm3, and are thus directly comparable with standard biochemical data.", "contents": "The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt. This article describes the use of a microdensitometer for the measurement of BPST formazan in tissue sections. BPST is a new tetrazolium salt, 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride, which produces a single, well-defined formazan, and is thus easily quantified. The formazan gives an excellent localization, since BPST was originally designed for ultrastructural work. Activities are expressed in absolute units as n moles hydrogen/mm3, and are thus directly comparable with standard biochemical data.", "PMID": 972071}
{"id": "pubmed23n0032_8804", "title": "The quantification of ormazans in tissue sections by microdensitometry. III. The effect of objective power and scanning spot size.", "content": "In this article, it is reported that the formazans derived from neotetrazolium chloride (NT) and 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride (BPST) can be measured by microdensitometry of tissue sections with a wide range of scanning spot sizes, without any significant effect on the recorded extinctions. The spot sizes tested ranged from 0.20 to 16 mum. Thus when large fields have to be measured, it is possible to use low-power objectives and still obtain valid results.", "contents": "The quantification of ormazans in tissue sections by microdensitometry. III. The effect of objective power and scanning spot size. In this article, it is reported that the formazans derived from neotetrazolium chloride (NT) and 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride (BPST) can be measured by microdensitometry of tissue sections with a wide range of scanning spot sizes, without any significant effect on the recorded extinctions. The spot sizes tested ranged from 0.20 to 16 mum. Thus when large fields have to be measured, it is possible to use low-power objectives and still obtain valid results.", "PMID": 972072}
{"id": "pubmed23n0032_8805", "title": "Acid phosphatase and peroxidase in \"resting\" acinar cells of the major salivary glands of cats and their possible movement into secretory granules.", "content": "After fixation of perarterial perfusion using an aldehyde mixture, salivary tissues were prepared for ultrastructural cytochemistry of acid phosphatase or peroxidase. Great variations in the distributions of the reaction product occurred, often within the same cell. Acid phosphatase staining occurred not only in lysosomes and sometimes in a GERL system, but a diffuse cytoplasmic component was also found in submandibular central acinar cells and to a lesser extent in parotid acini and variable staining occurred in the secretory granules of these cells. Peroxidase was variably associated with rough endoplasmic reticulum in submandibular demilunar cells, parotid acini, and more strongly in some sublingual cells. The secretory granules of the latter were darkly stained, but in parotid granules there was variable staining and least staining occurred in the granules of submandibular demilunes. These results are thought to indicate that not all enzymes present in secretory granules have reached there by an elective secretory process. Sometimes they appear to have entered the granules haphazardly, possibly having been enzymes associated with intracellular cisternal channels for transport or metabolism of other secretory substances and ultimately to have passed into the cisternal channels by chance or as part of a natural removal of redundant material.", "contents": "Acid phosphatase and peroxidase in \"resting\" acinar cells of the major salivary glands of cats and their possible movement into secretory granules. After fixation of perarterial perfusion using an aldehyde mixture, salivary tissues were prepared for ultrastructural cytochemistry of acid phosphatase or peroxidase. Great variations in the distributions of the reaction product occurred, often within the same cell. Acid phosphatase staining occurred not only in lysosomes and sometimes in a GERL system, but a diffuse cytoplasmic component was also found in submandibular central acinar cells and to a lesser extent in parotid acini and variable staining occurred in the secretory granules of these cells. Peroxidase was variably associated with rough endoplasmic reticulum in submandibular demilunar cells, parotid acini, and more strongly in some sublingual cells. The secretory granules of the latter were darkly stained, but in parotid granules there was variable staining and least staining occurred in the granules of submandibular demilunes. These results are thought to indicate that not all enzymes present in secretory granules have reached there by an elective secretory process. Sometimes they appear to have entered the granules haphazardly, possibly having been enzymes associated with intracellular cisternal channels for transport or metabolism of other secretory substances and ultimately to have passed into the cisternal channels by chance or as part of a natural removal of redundant material.", "PMID": 972073}
{"id": "pubmed23n0032_8806", "title": "Uridine diphosphoglucose dehydrogenase in the intervertebral disc.", "content": "Intervertebral discs of an old sheep and a young pig were examined for the presence of cells containing the enzyme uridine diphosphoglucose dehydrogenase. In the sheep, the inner anulus had a higher proportion of active cells than the outer anulus; in the pig, there was no difference. From a consideration of cell numbers, it is suggested that there is an accumulation of glycosaminoglycans in the centre of the disc rather than an increased production rate. Notochordal cells in the pig disc contain uridine diphosphoglucose dehydrogenase and are capable of producing glycosaminoglycans.", "contents": "Uridine diphosphoglucose dehydrogenase in the intervertebral disc. Intervertebral discs of an old sheep and a young pig were examined for the presence of cells containing the enzyme uridine diphosphoglucose dehydrogenase. In the sheep, the inner anulus had a higher proportion of active cells than the outer anulus; in the pig, there was no difference. From a consideration of cell numbers, it is suggested that there is an accumulation of glycosaminoglycans in the centre of the disc rather than an increased production rate. Notochordal cells in the pig disc contain uridine diphosphoglucose dehydrogenase and are capable of producing glycosaminoglycans.", "PMID": 972074}
{"id": "pubmed23n0032_8807", "title": "[Electro-cochleographic findings in posterior fossa tumours (author's transl)].", "content": "The different audiometric and electrocochleographic findings in two patients with acoustic neuromas are presented. Significantly different thresholds from the two methods of testing are explained on the basis of the ganglion cells, peripheral axons and hair cells remaining unaffected for some time whilst the central axons are damaged by tumour pressure. Differences of recruitment measured conventionally by ABLB audiometry or by electrocochleography are explained on a neurogenic basis. In cases of acoustic nerve fibre damage, single fibre tuning is altered to a raised threshold and a reduced frequency specificity.", "contents": "[Electro-cochleographic findings in posterior fossa tumours (author's transl)]. The different audiometric and electrocochleographic findings in two patients with acoustic neuromas are presented. Significantly different thresholds from the two methods of testing are explained on the basis of the ganglion cells, peripheral axons and hair cells remaining unaffected for some time whilst the central axons are damaged by tumour pressure. Differences of recruitment measured conventionally by ABLB audiometry or by electrocochleography are explained on a neurogenic basis. In cases of acoustic nerve fibre damage, single fibre tuning is altered to a raised threshold and a reduced frequency specificity.", "PMID": 972075}
{"id": "pubmed23n0032_8808", "title": "[How important are lower tunnels in the cottle septoplasty? (author's transl)].", "content": "The Cottle method is the best for correction of anterior nasal septal deformities. Establishment of the lower tunnel however seems unadvisable because of the common damage to the nasopalatine nerve passing through the incisive foramen with occasional permanent sequelae. The procedure also is time-consuming. As a rule spurs or deviations can be dealt with after establishing upper tunnels without perforation of the mucoperichondrial or mucoperiosteal flaps. The method is described in details.", "contents": "[How important are lower tunnels in the cottle septoplasty? (author's transl)]. The Cottle method is the best for correction of anterior nasal septal deformities. Establishment of the lower tunnel however seems unadvisable because of the common damage to the nasopalatine nerve passing through the incisive foramen with occasional permanent sequelae. The procedure also is time-consuming. As a rule spurs or deviations can be dealt with after establishing upper tunnels without perforation of the mucoperichondrial or mucoperiosteal flaps. The method is described in details.", "PMID": 972076}
{"id": "pubmed23n0032_8809", "title": "[Animal experiments on the influence of sarbitol on the damaged internal ear (author's transl)].", "content": "The influence of sorbitol was studied by recording the microphonic potentials (MP) of 10 guinea-pigs whose internal ears had been noise-damaged immediately before. Neither the rise of MP 10 sec after the start of noise exposure nor the total rise of MP showed any changes after the intraperitoneal injection of 2 ml of a 40% sorbitol solution.", "contents": "[Animal experiments on the influence of sarbitol on the damaged internal ear (author's transl)]. The influence of sorbitol was studied by recording the microphonic potentials (MP) of 10 guinea-pigs whose internal ears had been noise-damaged immediately before. Neither the rise of MP 10 sec after the start of noise exposure nor the total rise of MP showed any changes after the intraperitoneal injection of 2 ml of a 40% sorbitol solution.", "PMID": 972077}
{"id": "pubmed23n0032_8810", "title": "[Radiogenic sarcoma of the thyroid (author's transl)].", "content": "A sarcoma in the region of the thyroid, observed 30 years after radiotherapy in a patient with syringomyelia is presented. Even after low dose radiation in childhood and adolescence tumours of the thyroid frequently can be diagnosed. They rarely occur in adults, because apparently of a long latent period. This case is thought not to be a specific thyroid tumour.", "contents": "[Radiogenic sarcoma of the thyroid (author's transl)]. A sarcoma in the region of the thyroid, observed 30 years after radiotherapy in a patient with syringomyelia is presented. Even after low dose radiation in childhood and adolescence tumours of the thyroid frequently can be diagnosed. They rarely occur in adults, because apparently of a long latent period. This case is thought not to be a specific thyroid tumour.", "PMID": 972078}
{"id": "pubmed23n0032_8811", "title": "[Applied phoniatry. I. Retarded language and speech development (author's transl)].", "content": "To better understand the assessment of difficulties in verbal communication it is considered to explain language and speech development in relation to motor function. The aetiology, diagnosis, prognosis and treatment of language disorders and their common characteristics are discussed. The otolaryngologist must expect an increase in the number of such cases and should initiate prompt and adequate treatment.", "contents": "[Applied phoniatry. I. Retarded language and speech development (author's transl)]. To better understand the assessment of difficulties in verbal communication it is considered to explain language and speech development in relation to motor function. The aetiology, diagnosis, prognosis and treatment of language disorders and their common characteristics are discussed. The otolaryngologist must expect an increase in the number of such cases and should initiate prompt and adequate treatment.", "PMID": 972079}
{"id": "pubmed23n0032_8812", "title": "[Panel discussion: problems of the specialist's duty to inform the patient (author's transl)].", "content": "The obligation of the physician to inform the patient--which he has to prove in case of a suit--is based on the patient's right of self-determination. This self-decision information was subject of the panel discussion. Not discussed in detail were the information concerning diagnosis and prognosis, and the instruction of the patient regarding his conduct postoperatively and during medical treatment. Not considered was the so-called malpractice and negligence respectively. Medical liability suits are increasing for various reasons and are frequently directed at a failure to inform the patient becuase the patient is often unable to prove a negligence of the physician (\"surrogate liability\"). The dimension of the duty of disclosure (complete information--no information at all) is discussed in general and with special regard to the Ear-Nose- and Throat field. Special questions are answered regarding otoplasty, middle ear surgery, transplantations, extension of laryngeal surgery without prior informed consent, paranasal sinus and rhino-basis operations as well as surgery in minors and foreigners. In cases of non-vital indication and particularly in plastic-cosmetic procedures and outsider methods the requirements for informed consent are particularly important. The so-called rate of complications has perhaps a relative, but never an absolute meaning, and even this only in connection with the other circumstances of an individual case. The evidence of an adequate informed consent which must be included in a physician--patient-dialogue is most convincing by means of a written consent and the additional signature of a witness, perhaps also of the patient. The value, the problems and even the risks of an information based only on forms or brochures is discussed in detail. The panel discussion from the four points of view of the organizers should be no means confuse the otorhinolaryngologist. It should inform him about the medicolegal aspects of his activity and protect him from avoidable burdens.", "contents": "[Panel discussion: problems of the specialist's duty to inform the patient (author's transl)]. The obligation of the physician to inform the patient--which he has to prove in case of a suit--is based on the patient's right of self-determination. This self-decision information was subject of the panel discussion. Not discussed in detail were the information concerning diagnosis and prognosis, and the instruction of the patient regarding his conduct postoperatively and during medical treatment. Not considered was the so-called malpractice and negligence respectively. Medical liability suits are increasing for various reasons and are frequently directed at a failure to inform the patient becuase the patient is often unable to prove a negligence of the physician (\"surrogate liability\"). The dimension of the duty of disclosure (complete information--no information at all) is discussed in general and with special regard to the Ear-Nose- and Throat field. Special questions are answered regarding otoplasty, middle ear surgery, transplantations, extension of laryngeal surgery without prior informed consent, paranasal sinus and rhino-basis operations as well as surgery in minors and foreigners. In cases of non-vital indication and particularly in plastic-cosmetic procedures and outsider methods the requirements for informed consent are particularly important. The so-called rate of complications has perhaps a relative, but never an absolute meaning, and even this only in connection with the other circumstances of an individual case. The evidence of an adequate informed consent which must be included in a physician--patient-dialogue is most convincing by means of a written consent and the additional signature of a witness, perhaps also of the patient. The value, the problems and even the risks of an information based only on forms or brochures is discussed in detail. The panel discussion from the four points of view of the organizers should be no means confuse the otorhinolaryngologist. It should inform him about the medicolegal aspects of his activity and protect him from avoidable burdens.", "PMID": 972080}
{"id": "pubmed23n0032_8813", "title": "[Transtympanic neurectomy of the vestibular and cochlear nerves (author's transl)].", "content": "Tympanotomy and a transvestibular approach to the fundus of the internal auditory meatus is described. The surgical technique is modified from that proposed by Alvarez de Cozar and Antoli-Candela in 1970. The surgical anatomy of the operative sites is detailed. The surgical procedure is found indicated in patients in whom preservation of hearing is not considered and vestibular neurectomy required. The described technique provides a simple but efficient and safe alternative to the translabyrinthine approach to the internal auditory meatus for division of the vestibular and cochlear nerves.", "contents": "[Transtympanic neurectomy of the vestibular and cochlear nerves (author's transl)]. Tympanotomy and a transvestibular approach to the fundus of the internal auditory meatus is described. The surgical technique is modified from that proposed by Alvarez de Cozar and Antoli-Candela in 1970. The surgical anatomy of the operative sites is detailed. The surgical procedure is found indicated in patients in whom preservation of hearing is not considered and vestibular neurectomy required. The described technique provides a simple but efficient and safe alternative to the translabyrinthine approach to the internal auditory meatus for division of the vestibular and cochlear nerves.", "PMID": 972081}
{"id": "pubmed23n0032_8814", "title": "[The lombard reflex as a test of vocal function (author's transl)].", "content": "Any impairment of audio-phonatory control by background noise is followed by an increase in both the intensity and pitch of the speaking voice (Lombard reflex, 1911), thus increasing vocal strain. As a consequence, it might be anticipated that persons reacting to noise with marked changes in voice might be more liable to develop dysphonia. 22 singers, 34 normal controls, and 22 patients with hyperfunctional dysphonia where studied. In all patients, both ears were gradually masked with white noise. The change of the mean intensity level and of the mean pitch level of the speaking voice were then measured objectively with a special fundamental frequency analyzer (Fedders and Schultz-Coulon, 1975). Results show that the increase of intensity is comparable in all subjects, whereas the elevation of the mean pitch level differs significantly: trained voices (singers) react with the least pitch increment whereas dysphonic patients react with the most. The following conclusions were made from the present investigation: 1. Extreme increments in pitch level can be considered to be a more significant etiological factor of dysphonia than intensity increments; 2. Vocal therapy and voice training may have a favorable effect on the Lombard reflex (probably by improvement of the kinesthetic control mechanism) so that the speaking voice in a noisy environment is raised less with less vocal strain. The study also indicates that measurement of pitch changes during binaural masking can provide important information for the diagnosis, therapy and prophylaxis of dysphonia.", "contents": "[The lombard reflex as a test of vocal function (author's transl)]. Any impairment of audio-phonatory control by background noise is followed by an increase in both the intensity and pitch of the speaking voice (Lombard reflex, 1911), thus increasing vocal strain. As a consequence, it might be anticipated that persons reacting to noise with marked changes in voice might be more liable to develop dysphonia. 22 singers, 34 normal controls, and 22 patients with hyperfunctional dysphonia where studied. In all patients, both ears were gradually masked with white noise. The change of the mean intensity level and of the mean pitch level of the speaking voice were then measured objectively with a special fundamental frequency analyzer (Fedders and Schultz-Coulon, 1975). Results show that the increase of intensity is comparable in all subjects, whereas the elevation of the mean pitch level differs significantly: trained voices (singers) react with the least pitch increment whereas dysphonic patients react with the most. The following conclusions were made from the present investigation: 1. Extreme increments in pitch level can be considered to be a more significant etiological factor of dysphonia than intensity increments; 2. Vocal therapy and voice training may have a favorable effect on the Lombard reflex (probably by improvement of the kinesthetic control mechanism) so that the speaking voice in a noisy environment is raised less with less vocal strain. The study also indicates that measurement of pitch changes during binaural masking can provide important information for the diagnosis, therapy and prophylaxis of dysphonia.", "PMID": 972082}
{"id": "pubmed23n0032_8815", "title": "[The clinical olfactogram (author's transl)].", "content": "Clinical olfactometry is described in which patients are exposed to supraliminal stimuli of well-known types of odors. The results are then placed on a graded recognition scale as a polar coordinate system. Each clinical odor tested produces a typical recognition pattern which can easily be interpreted. Typical patterns for different conditions involving olfaction exist, and include euosmia, sensory anosmia, and respiratory anosmia. A simplified U-tube rhinomanometer can also be used, and data graphed on the same chart as for olfactometry. The procedure as described has been successfully tested on several hundred patients and controls.", "contents": "[The clinical olfactogram (author's transl)]. Clinical olfactometry is described in which patients are exposed to supraliminal stimuli of well-known types of odors. The results are then placed on a graded recognition scale as a polar coordinate system. Each clinical odor tested produces a typical recognition pattern which can easily be interpreted. Typical patterns for different conditions involving olfaction exist, and include euosmia, sensory anosmia, and respiratory anosmia. A simplified U-tube rhinomanometer can also be used, and data graphed on the same chart as for olfactometry. The procedure as described has been successfully tested on several hundred patients and controls.", "PMID": 972083}
{"id": "pubmed23n0032_8816", "title": "Visceral and somatic reflexes produced by J pulmonary receptors in newborn kittens.", "content": "The functional efficiency of J pulmonary receptor reflexes was studied in 28 newborn anesthetized kittens. J receptor stimulation was achieved by injecting phenyl diguanide (PDG) solution into the right atrium using a technique established earlier for cats. Control injections of PDG into the aorta were made. Respiration, aortic blood presure, and knee jerk were recorded. The Hering-Breuer reflex was routinely tested in each kitten by inflating the lungs through the tracheal cannula. In the newborn kitten (1-6 days old), it was not possible to elicit any effect on injection of PDG into the right atrium. At 1 wk, the visceral reflexes--apnea, rapid shallow breathing, bradycardia--could be produced by doses much higher (six times) than the adult dose. By the 10th day, the visceral reflexes were fully developed. The motor reflex, i.e., inhibition of EMG and reduction of knee jerk, was elicited after 3 wk. The earliest response was seen in a 3-wk-old kitten. All these reflexes were abolished by bilateral cervical vagotomy. These results suggest that the J receptor reflexes are poorly developed in the newborn kitten and that the motor reflexes develop much later than the visceral reflexes.", "contents": "Visceral and somatic reflexes produced by J pulmonary receptors in newborn kittens. The functional efficiency of J pulmonary receptor reflexes was studied in 28 newborn anesthetized kittens. J receptor stimulation was achieved by injecting phenyl diguanide (PDG) solution into the right atrium using a technique established earlier for cats. Control injections of PDG into the aorta were made. Respiration, aortic blood presure, and knee jerk were recorded. The Hering-Breuer reflex was routinely tested in each kitten by inflating the lungs through the tracheal cannula. In the newborn kitten (1-6 days old), it was not possible to elicit any effect on injection of PDG into the right atrium. At 1 wk, the visceral reflexes--apnea, rapid shallow breathing, bradycardia--could be produced by doses much higher (six times) than the adult dose. By the 10th day, the visceral reflexes were fully developed. The motor reflex, i.e., inhibition of EMG and reduction of knee jerk, was elicited after 3 wk. The earliest response was seen in a 3-wk-old kitten. All these reflexes were abolished by bilateral cervical vagotomy. These results suggest that the J receptor reflexes are poorly developed in the newborn kitten and that the motor reflexes develop much later than the visceral reflexes.", "PMID": 972120}
{"id": "pubmed23n0032_8817", "title": "A new method to determine frequency characteristics of the respiratory system.", "content": "A technique is described allowing one to determine simultaneously the resistance and reactance of the total respiratory system for various frequencies. During spontaneous breathing, regularly recurring impulses are produced at the mouth by means of a loud speaker. A Fourier analysis of the mouth pressure and flow signals yields mean resistance and reactance values, over 16 s, for all harmonics of 2 Hz up to 30 Hz. The values are in good agreement with those obtained in the absence of breathing and those determined by means of the forced oscillation technique and by body plethysmography. The reproducibility of the measurements is satisfactory (coefficient of variation: 11.6%).", "contents": "A new method to determine frequency characteristics of the respiratory system. A technique is described allowing one to determine simultaneously the resistance and reactance of the total respiratory system for various frequencies. During spontaneous breathing, regularly recurring impulses are produced at the mouth by means of a loud speaker. A Fourier analysis of the mouth pressure and flow signals yields mean resistance and reactance values, over 16 s, for all harmonics of 2 Hz up to 30 Hz. The values are in good agreement with those obtained in the absence of breathing and those determined by means of the forced oscillation technique and by body plethysmography. The reproducibility of the measurements is satisfactory (coefficient of variation: 11.6%).", "PMID": 972121}
{"id": "pubmed23n0032_8818", "title": "An improved plethysmograph with servo control of hydrostatic pressure.", "content": "One limitation of the conventional fluid displacement plethysmograph is that the hydrostatic pressure varies as recordings of volume change are obtained. Thus, use of this instrument can lead to inaccuracies that are proportional to the compliance of the biological system under study. This paper describes an improved design which utilizes a servo-control system and an open, mobile reservoir to ensure that the hydrostatic pressure is maintained constant as volume changes are recorded. The result is a device that succeeds in combining stability, sensitivity and accuracy in a fluid displacement plethysmograph.", "contents": "An improved plethysmograph with servo control of hydrostatic pressure. One limitation of the conventional fluid displacement plethysmograph is that the hydrostatic pressure varies as recordings of volume change are obtained. Thus, use of this instrument can lead to inaccuracies that are proportional to the compliance of the biological system under study. This paper describes an improved design which utilizes a servo-control system and an open, mobile reservoir to ensure that the hydrostatic pressure is maintained constant as volume changes are recorded. The result is a device that succeeds in combining stability, sensitivity and accuracy in a fluid displacement plethysmograph.", "PMID": 972122}
{"id": "pubmed23n0032_8819", "title": "Implantable electrode for recording nerve signals in awake animals.", "content": "An implantable electrode assembly consisting of collagen and metallic electrodes was constructed to measure simultaneously neural signals from the intact nerve and bioelectrical noises in awake animals. Mechanical artifacts, due to bodily movement, were negligibly small. The impedance of the collagen electrodes, measured in awake cats 6-7 days after implantation surgery, ranged from 39.8-11.5 komega at a frequency range of 20-5 kHz. Aortic nerve activity and renal nerve activity, measured in awake conditions using the collagen electrode, showed grouped activity synchronous with the cardiac cycle. Results indicate that most of the remal nerve activity was from postganglionic sympathetic fibers and was inhibited by the baroceptor reflex in the same cardiac cycle.", "contents": "Implantable electrode for recording nerve signals in awake animals. An implantable electrode assembly consisting of collagen and metallic electrodes was constructed to measure simultaneously neural signals from the intact nerve and bioelectrical noises in awake animals. Mechanical artifacts, due to bodily movement, were negligibly small. The impedance of the collagen electrodes, measured in awake cats 6-7 days after implantation surgery, ranged from 39.8-11.5 komega at a frequency range of 20-5 kHz. Aortic nerve activity and renal nerve activity, measured in awake conditions using the collagen electrode, showed grouped activity synchronous with the cardiac cycle. Results indicate that most of the remal nerve activity was from postganglionic sympathetic fibers and was inhibited by the baroceptor reflex in the same cardiac cycle.", "PMID": 972123}
{"id": "pubmed23n0032_8820", "title": "Gas transport in a model derived from Hansen-Ampaya anatomical data of the human lung.", "content": "The anatomical data of the human lung published by Hansen and Ampaya are used in a model of gas transport in the lung. The Bohr dead space is calculated from solutions of a transport equation where diffusivity is given by an empirical formula obtained by Sherer et al. A satisfactory agreement is found with experimental data obtained from simultaneous washouts of H2 and SF6 for respiratory frequencies ranging between 15 and 60 min-1 and tidal volumes between 200 and 1,800 ml. The results support the idea that molecular diffusion is the main but not the only physical phenomenom which intervenes in gas mixing during breathing.", "contents": "Gas transport in a model derived from Hansen-Ampaya anatomical data of the human lung. The anatomical data of the human lung published by Hansen and Ampaya are used in a model of gas transport in the lung. The Bohr dead space is calculated from solutions of a transport equation where diffusivity is given by an empirical formula obtained by Sherer et al. A satisfactory agreement is found with experimental data obtained from simultaneous washouts of H2 and SF6 for respiratory frequencies ranging between 15 and 60 min-1 and tidal volumes between 200 and 1,800 ml. The results support the idea that molecular diffusion is the main but not the only physical phenomenom which intervenes in gas mixing during breathing.", "PMID": 972124}
{"id": "pubmed23n0032_8821", "title": "Pneumotachograph for flow and volume measurement in normal and dense atmospheres.", "content": "Measurement of ventilatory flow during pulmonary function maneuvers and in severe exercise by the pneumotachograph flow transducer in dense atmospheres is handicapped by the inverse relationship between the linear range of such transducers and gas density. This limitation in the usefulness of the pneumotachograph can be overcome by mounting such flow transducers in parallel and taking into account the reduction in peak ventilatory flow transducers in parallel and taking into account the reduction in peak ventilatory flow which occurs with increasing density. The design of such assemblies, their static performance characteristics, and a drift subtractor, constructed to stabilize the output of the pneumotachograph pressure transducer, are described.", "contents": "Pneumotachograph for flow and volume measurement in normal and dense atmospheres. Measurement of ventilatory flow during pulmonary function maneuvers and in severe exercise by the pneumotachograph flow transducer in dense atmospheres is handicapped by the inverse relationship between the linear range of such transducers and gas density. This limitation in the usefulness of the pneumotachograph can be overcome by mounting such flow transducers in parallel and taking into account the reduction in peak ventilatory flow transducers in parallel and taking into account the reduction in peak ventilatory flow which occurs with increasing density. The design of such assemblies, their static performance characteristics, and a drift subtractor, constructed to stabilize the output of the pneumotachograph pressure transducer, are described.", "PMID": 972125}
{"id": "pubmed23n0032_8822", "title": "Nocturnal lowering of thresholds for sweating and vasodilation.", "content": "Six subjects exercised on a bicycle ergometer at 60-70% of maximal aerobic power in a 25 degrees C ambient. Experiments on each subject were conducted at night (4:00-5:30 A.M.) and in daytime (noon-4:30 P.M.). Chest sweating rate (msw) was measured with resistance hygrometry. Forearm blood flow (BF), with  an arm skin temperature of 35.5 +/- 1.2 degrees C (SD), was measured with electrocapacitance plethysmography. Esophageal temperature (Tes) was measured with a thermocouple at the level of the left atrium, and mean skin temperature (Tsk) was calculated from a weighted average of temperatures at three sites. Tes was corrected to a skin temperature of 33 degrees C as follows: T'es = Tes + (Tsk - 33 degrees C)/8. This correction reflects the relative contributions of Tes and Tsk to control of msw:T'es and BF:T'es relations were not consistently changed. In any given subject, thresholds for sweating and vasodilation were shifted about equally. These shifts averaged 0.57 degrees C (range: 0.23-0.93 degrees C)for msw and 0.63 degrees C (range: 0.17-0.98 degrees C) for BF.", "contents": "Nocturnal lowering of thresholds for sweating and vasodilation. Six subjects exercised on a bicycle ergometer at 60-70% of maximal aerobic power in a 25 degrees C ambient. Experiments on each subject were conducted at night (4:00-5:30 A.M.) and in daytime (noon-4:30 P.M.). Chest sweating rate (msw) was measured with resistance hygrometry. Forearm blood flow (BF), with  an arm skin temperature of 35.5 +/- 1.2 degrees C (SD), was measured with electrocapacitance plethysmography. Esophageal temperature (Tes) was measured with a thermocouple at the level of the left atrium, and mean skin temperature (Tsk) was calculated from a weighted average of temperatures at three sites. Tes was corrected to a skin temperature of 33 degrees C as follows: T'es = Tes + (Tsk - 33 degrees C)/8. This correction reflects the relative contributions of Tes and Tsk to control of msw:T'es and BF:T'es relations were not consistently changed. In any given subject, thresholds for sweating and vasodilation were shifted about equally. These shifts averaged 0.57 degrees C (range: 0.23-0.93 degrees C)for msw and 0.63 degrees C (range: 0.17-0.98 degrees C) for BF.", "PMID": 972126}
{"id": "pubmed23n0032_8823", "title": "Sex and age differences in pulmonary mechanics in normal nonsmoking subjects.", "content": "Maximun flow-volume, static pressure-volume, and maximum flow static recoil curves of three groups of nonsmoking, normal subjects (young men, young women, elderly women) were used to assess age and sex differences in pulmonary mechanics. No significant sex differences in maximum flow were seen but the young men showed higher lung recoil pressures at full inflation. When the influence of the inspiratory muscles and chest wall was excluded by exponential extrapolation of the pressure-volume curves to a maximum volume the bulk elastic properties of the lungs of young men and women appeared identical. Loss of maximum expiratory flow at low lung volumes and of lung recoil pressure occurred with age in nonsmoking women in whom emphysema should be minimal and therefore indicate true physiological effects of aging. The changes in pulmonary mechanics with age are consistent with an increase in unstressed dimensions and loss of elastic recoil of both alveoli and airways.", "contents": "Sex and age differences in pulmonary mechanics in normal nonsmoking subjects. Maximun flow-volume, static pressure-volume, and maximum flow static recoil curves of three groups of nonsmoking, normal subjects (young men, young women, elderly women) were used to assess age and sex differences in pulmonary mechanics. No significant sex differences in maximum flow were seen but the young men showed higher lung recoil pressures at full inflation. When the influence of the inspiratory muscles and chest wall was excluded by exponential extrapolation of the pressure-volume curves to a maximum volume the bulk elastic properties of the lungs of young men and women appeared identical. Loss of maximum expiratory flow at low lung volumes and of lung recoil pressure occurred with age in nonsmoking women in whom emphysema should be minimal and therefore indicate true physiological effects of aging. The changes in pulmonary mechanics with age are consistent with an increase in unstressed dimensions and loss of elastic recoil of both alveoli and airways.", "PMID": 972127}
{"id": "pubmed23n0032_8824", "title": "Plasma renin, angiotensin II, and plasma and urinary aldosterone in running exercise.", "content": "Plasma renin activity (PRA), renin concentration (PRC), angiotensin II and urinary aldosterone of four male athletes were investigated before and after a running exercise of 3 X 300 m. After the exercise, there were marked increases in all these parameters. The maximal increases (of the means and the ranges), found in the samples taken 30 min after the exercise, were: 108% (27-230%, P less than 0.05) in PRA, 490% (240-800%, P less than 0.01) in PRC, 830% (400-1,970%, P less than 0.025) in plasma angiotensin II and 1,600% (160-3,920%, P less than 0.02) in plasma aldosterone. The increase in the urinary excretion of aldosterone was 120% (42-180%, P less than 0.025). This study demonstrates that intense physical exercise may cause marked changes in all the three main components of the renin-angiotensin-aldosterone system. The significance of these changes for the physiological function of the human organism in physical stress needs further investigation.", "contents": "Plasma renin, angiotensin II, and plasma and urinary aldosterone in running exercise. Plasma renin activity (PRA), renin concentration (PRC), angiotensin II and urinary aldosterone of four male athletes were investigated before and after a running exercise of 3 X 300 m. After the exercise, there were marked increases in all these parameters. The maximal increases (of the means and the ranges), found in the samples taken 30 min after the exercise, were: 108% (27-230%, P less than 0.05) in PRA, 490% (240-800%, P less than 0.01) in PRC, 830% (400-1,970%, P less than 0.025) in plasma angiotensin II and 1,600% (160-3,920%, P less than 0.02) in plasma aldosterone. The increase in the urinary excretion of aldosterone was 120% (42-180%, P less than 0.025). This study demonstrates that intense physical exercise may cause marked changes in all the three main components of the renin-angiotensin-aldosterone system. The significance of these changes for the physiological function of the human organism in physical stress needs further investigation.", "PMID": 972128}
{"id": "pubmed23n0032_8825", "title": "Automic innervation of dog coronary arteries.", "content": "The autonomic innervation of canine coronary arteries has been examined using the Falck and Owman technique for demonstrating catecholamines and a modification of the Koelle technique for the demonstration of cholinesterase. The experimental protocol included an examination of the neural innervation of the major coronary arteries: LCC, LAD, and RCA. A consistent, relatively dense adrenergic innervation was noted. A gradient in the degree of cholinergic innervation was: LAD  less than RCA less than LCC. Light microscopic examination of the hearts of dogs subjected to either cervical vagotomy or total extrinsic cardiac denervation was performed. Additional surgical procedures included removal of the left stellate ganglion and a preferential stripping of the LCC. These studies demonstrated the intrinsic nature of parasympathetic coronary innervation. Following all surgical procedure no variations in density of cholinergic innervation were noted, indicating that these fibers are probably postganglionic parasympathetic fibers arising from intrinsic ganglia within the ventricles. These ganglia may be located at the base of the great vessels and send their fibers to the coronary vessels via the septal artery.", "contents": "Automic innervation of dog coronary arteries. The autonomic innervation of canine coronary arteries has been examined using the Falck and Owman technique for demonstrating catecholamines and a modification of the Koelle technique for the demonstration of cholinesterase. The experimental protocol included an examination of the neural innervation of the major coronary arteries: LCC, LAD, and RCA. A consistent, relatively dense adrenergic innervation was noted. A gradient in the degree of cholinergic innervation was: LAD  less than RCA less than LCC. Light microscopic examination of the hearts of dogs subjected to either cervical vagotomy or total extrinsic cardiac denervation was performed. Additional surgical procedures included removal of the left stellate ganglion and a preferential stripping of the LCC. These studies demonstrated the intrinsic nature of parasympathetic coronary innervation. Following all surgical procedure no variations in density of cholinergic innervation were noted, indicating that these fibers are probably postganglionic parasympathetic fibers arising from intrinsic ganglia within the ventricles. These ganglia may be located at the base of the great vessels and send their fibers to the coronary vessels via the septal artery.", "PMID": 972129}
{"id": "pubmed23n0032_8826", "title": "Maturational effects on respiratory responses to carbon dioxide in premature infants.", "content": "The respiratory responses of 18 premature infants to breathing steady-state levels of carbon dioxide were quantified by conventional minute ventilation measurements and by measuring the pressure generated after end-expiratory airway occlusions. Since no flow or volume changes occur following airway occlusion, the CO2 response obtained by this measurement is independent of vagal stretch receptor influence and mechanical changes in the lungs. When studied at 3-4 days of postnatal age, a group of 29-32-wk gestation infants had a mean slope of ventilatory response to CO2 of 10.8 +/- 5.6 ml-min-1-kg-1-mmHg-1 as compared to 53.5 +/- 10.8 ml-min-1-kg-1-mmHg-1 (P less than 0.005) for a group of 33-36 wk. When these infants were restudied at 7-10 days of age, the slopes were 21.6+/- 6.6 and 58.4 +/- 10.6 ml-min-1-kg-1-mmHg-1 (P less than 0.01), respectively. The mask pressure response paralleled minute ventilation. We conclude that the increase in ventilatory response to carbon dioxide seen with maturation is not due only to changing mechanical features of the lungs, but to increased sensitivity of respiratory centers to carbon dioxide.", "contents": "Maturational effects on respiratory responses to carbon dioxide in premature infants. The respiratory responses of 18 premature infants to breathing steady-state levels of carbon dioxide were quantified by conventional minute ventilation measurements and by measuring the pressure generated after end-expiratory airway occlusions. Since no flow or volume changes occur following airway occlusion, the CO2 response obtained by this measurement is independent of vagal stretch receptor influence and mechanical changes in the lungs. When studied at 3-4 days of postnatal age, a group of 29-32-wk gestation infants had a mean slope of ventilatory response to CO2 of 10.8 +/- 5.6 ml-min-1-kg-1-mmHg-1 as compared to 53.5 +/- 10.8 ml-min-1-kg-1-mmHg-1 (P less than 0.005) for a group of 33-36 wk. When these infants were restudied at 7-10 days of age, the slopes were 21.6+/- 6.6 and 58.4 +/- 10.6 ml-min-1-kg-1-mmHg-1 (P less than 0.01), respectively. The mask pressure response paralleled minute ventilation. We conclude that the increase in ventilatory response to carbon dioxide seen with maturation is not due only to changing mechanical features of the lungs, but to increased sensitivity of respiratory centers to carbon dioxide.", "PMID": 972130}
{"id": "pubmed23n0032_8827", "title": "Physiological aging of champion runners.", "content": "The physiological adjustments to aerobic work (5.6 km/h, up a 9% grade) and to exhausting treadmill work of former champion middle-distance runners were determined in 1971, at ages 47-68 yr, 25-43 yr after their competitive careers in track. In the resting state the former athletes as a group are very much like nonathletes of the same ages. Efficiency in the aerobic walk was the same in both groups and did not change with age in either, but the former athletes on the average performed the walk with less strain as indicated by lower blood lactates, \"ventilatory equivalents,\" and heart rates than nonathletes at corresponding ages. Mean VO2max of the runners declined from 71.4 ml/min-kg-1 in youth to 41.8 at a mean age of 56.6 yr, as compared with mean values of 50.6 and 36.5 ml/min-kg-1 in nonathletes at corresponding ages. VO2max had declined below the average of nonathletic men in only two of the former runners. Mean maximal heart rate declined with age from 186 to 180 in the runners, and from 199 to 186 in nonathletes at corresponding ages. Ventilatory responses of men in both groups were closely related to the increases of blood lactate in both aerobic and maximal work.", "contents": "Physiological aging of champion runners. The physiological adjustments to aerobic work (5.6 km/h, up a 9% grade) and to exhausting treadmill work of former champion middle-distance runners were determined in 1971, at ages 47-68 yr, 25-43 yr after their competitive careers in track. In the resting state the former athletes as a group are very much like nonathletes of the same ages. Efficiency in the aerobic walk was the same in both groups and did not change with age in either, but the former athletes on the average performed the walk with less strain as indicated by lower blood lactates, \"ventilatory equivalents,\" and heart rates than nonathletes at corresponding ages. Mean VO2max of the runners declined from 71.4 ml/min-kg-1 in youth to 41.8 at a mean age of 56.6 yr, as compared with mean values of 50.6 and 36.5 ml/min-kg-1 in nonathletes at corresponding ages. VO2max had declined below the average of nonathletic men in only two of the former runners. Mean maximal heart rate declined with age from 186 to 180 in the runners, and from 199 to 186 in nonathletes at corresponding ages. Ventilatory responses of men in both groups were closely related to the increases of blood lactate in both aerobic and maximal work.", "PMID": 972131}
{"id": "pubmed23n0032_8828", "title": "Systolic time intervals during +Gz acceleration.", "content": "Systolic time intervals (STI) were recorded in 8 healthy male volunteers before, during, and after 30-s exposures to +3 Gz, +5 Gz, and +7 Gz acceleration. Heart rate (HR) increased at all +Gz levels, as did the HR corrected QSIc interval, left ventricular ejection time (LVETc), preejection period (PEPc) and PEP/LVET. These changes in STI were also proportional to the +Gz level. At the higher +Gz levels, PEPc and PEP/LVET continued to increase early in the recovery period, but HR and all STI returned to control after 60 s of recovery. Although physiological variables other than myocardial contractility, such as preload and afterload may influence STI during +Gz the effects of +Gz on stroke volume (SV) and cardiac output (CO) were estimated using previously described relationships between STI and invasively determined indices of cardiovascular function. In general CO increased as SV decreased. During recovery, HR and CO fell and CO remained slightly below control levels, primarily because estimated SV remained low. This study demonstrates the feasibility of using STI to estimate noninvasively the transient changes in cardiovascular function during +Gz acceleration.", "contents": "Systolic time intervals during +Gz acceleration. Systolic time intervals (STI) were recorded in 8 healthy male volunteers before, during, and after 30-s exposures to +3 Gz, +5 Gz, and +7 Gz acceleration. Heart rate (HR) increased at all +Gz levels, as did the HR corrected QSIc interval, left ventricular ejection time (LVETc), preejection period (PEPc) and PEP/LVET. These changes in STI were also proportional to the +Gz level. At the higher +Gz levels, PEPc and PEP/LVET continued to increase early in the recovery period, but HR and all STI returned to control after 60 s of recovery. Although physiological variables other than myocardial contractility, such as preload and afterload may influence STI during +Gz the effects of +Gz on stroke volume (SV) and cardiac output (CO) were estimated using previously described relationships between STI and invasively determined indices of cardiovascular function. In general CO increased as SV decreased. During recovery, HR and CO fell and CO remained slightly below control levels, primarily because estimated SV remained low. This study demonstrates the feasibility of using STI to estimate noninvasively the transient changes in cardiovascular function during +Gz acceleration.", "PMID": 972132}
{"id": "pubmed23n0032_8829", "title": "Thermoregulatory response of women to intermittent work in the heat.", "content": "Seven women worked intermittently in three randomly ordered sessions at 75% Vo2max at three temperatures, 28 degrees C (45% rh) 35 degrees C (65% rh), and 48 degrees C (10% rh) and recovered in a cool environment (22 degrees C) after each 6-min work period. Although Tre was higher in each successive work period, the ambient temperature had no effect on the cardiovascular or respiratory responses or on deltaTre. In all conditions SV decreased with time with a concomitant increase in HR to maintain Q. A fall in mean blood pressure from the initial to final measurement was due entirely to a decrease in diastolic pressure. The final Tre for these women was approximately equal to that previously reported for men working continuously for 1 h under conditions equivalent to time-weighted average of the thermal and metabolic loads during work and recovery in this study.", "contents": "Thermoregulatory response of women to intermittent work in the heat. Seven women worked intermittently in three randomly ordered sessions at 75% Vo2max at three temperatures, 28 degrees C (45% rh) 35 degrees C (65% rh), and 48 degrees C (10% rh) and recovered in a cool environment (22 degrees C) after each 6-min work period. Although Tre was higher in each successive work period, the ambient temperature had no effect on the cardiovascular or respiratory responses or on deltaTre. In all conditions SV decreased with time with a concomitant increase in HR to maintain Q. A fall in mean blood pressure from the initial to final measurement was due entirely to a decrease in diastolic pressure. The final Tre for these women was approximately equal to that previously reported for men working continuously for 1 h under conditions equivalent to time-weighted average of the thermal and metabolic loads during work and recovery in this study.", "PMID": 972133}
{"id": "pubmed23n0032_8830", "title": "Threshold temperatures for shivering in acute and chronic hypercapnia.", "content": "Threshold temperatures for shivering in acute and chronic hypercapnia were determined in guinea pigs by measuring the time course of cervical cord temperature, skin temperature, oxygen consumption (Vo2), and electrical muscle activity during cold exposure (15 degrees C). Prior to acute exposure to CO2, the shivering threshold was determined in each animal during control conditions breathing air. With increasing CO2 concentrations (5,7.5, and 15% CO2) the shivering thresholds fell to lower temperatures, decreasing by approximately 40 degrees C at 15% CO2. The shift of the shivering threshold to lower values found during acute exposure to 15% CO2 was reversed after chronic exposure to 15% CO2 for 3 days, which marks the time of metabolic adaptation to CO2.", "contents": "Threshold temperatures for shivering in acute and chronic hypercapnia. Threshold temperatures for shivering in acute and chronic hypercapnia were determined in guinea pigs by measuring the time course of cervical cord temperature, skin temperature, oxygen consumption (Vo2), and electrical muscle activity during cold exposure (15 degrees C). Prior to acute exposure to CO2, the shivering threshold was determined in each animal during control conditions breathing air. With increasing CO2 concentrations (5,7.5, and 15% CO2) the shivering thresholds fell to lower temperatures, decreasing by approximately 40 degrees C at 15% CO2. The shift of the shivering threshold to lower values found during acute exposure to 15% CO2 was reversed after chronic exposure to 15% CO2 for 3 days, which marks the time of metabolic adaptation to CO2.", "PMID": 972134}
{"id": "pubmed23n0032_8831", "title": "Physiologic limits to work in the heat and evaporative coefficient for women.", "content": "Ten heat-acclimated females exercised seminude on a treadmill at 30% Vo2 max (M=152 W-m-2) under eight air temperatures (Ta) ranging from 30 degrees C to 52 degrees C. Each experiment involved 1 h of fixed and a 2nd h of progressively increasing water vapor pressure (Pw) with either air movement of 1 m-s-1 or still air. The equilibrium values of rectal temperature (Tre), mean skin temperature (Tsk),and heart rate (HR) reached in the 1st h were forced upwards in the 2nd h by the rising Pw. The critical Pw was defined by the Tre inflection point for each Ta. The loci of the critical Pw were used to delineate the thermal limits on the psychrometric chart and were used to derive the effective evaporative coefficient (Ke') applicable to the ambient capacity for evaporative cooling (Emax). The derived Ke' was 17.6 +/- 4.2 W-m-2 (mean +/- SD) for v0.6m-s-1. Isotherms constructed on the basis of the obtained K\u00e9, Tsk, and sweating capacity were higher than the physiologically based Pw limits.", "contents": "Physiologic limits to work in the heat and evaporative coefficient for women. Ten heat-acclimated females exercised seminude on a treadmill at 30% Vo2 max (M=152 W-m-2) under eight air temperatures (Ta) ranging from 30 degrees C to 52 degrees C. Each experiment involved 1 h of fixed and a 2nd h of progressively increasing water vapor pressure (Pw) with either air movement of 1 m-s-1 or still air. The equilibrium values of rectal temperature (Tre), mean skin temperature (Tsk),and heart rate (HR) reached in the 1st h were forced upwards in the 2nd h by the rising Pw. The critical Pw was defined by the Tre inflection point for each Ta. The loci of the critical Pw were used to delineate the thermal limits on the psychrometric chart and were used to derive the effective evaporative coefficient (Ke') applicable to the ambient capacity for evaporative cooling (Emax). The derived Ke' was 17.6 +/- 4.2 W-m-2 (mean +/- SD) for v0.6m-s-1. Isotherms constructed on the basis of the obtained K\u00e9, Tsk, and sweating capacity were higher than the physiologically based Pw limits.", "PMID": 972135}
{"id": "pubmed23n0032_8832", "title": "Effects of body weight and food intake on pleasantness ratings for a sweet stimulus.", "content": "A physiological role for pleasure has been suggested from observations that glucose loads reduced pleasantness ratings for sweet stimuli in normal weight but not in weight-reduced subjects. This hypothesis was reexamined in fasting and food-loaded, lean and obese subjects. Magnitude estimates of sweetness and pleasantness were obtained for seven sucrose concentrations. Indices of obesity including percent overweight, percent body fat, and body mass index were calculated. Pleasantness ratings of fasting subjects either increased up to a moderate concentration and then declined (type I hedonic response) or increased monotonically with concentration (type II hedonic response). Both types of response were found in lean and obese subjects. Within each weight group there appeared a negative correlation between pleasantness response and indices of obesity. Caloric loads reduced hedonic ratings of type II lean and obese subjects, but did not affect pleasantness response of type I lean and obese subjects. These findings indicate the presence of an hedonic monitor biased by body weight and caloric intake. The data suggest a physiological role for pleasure in regulating body weight at different set points in lean and obese subjects.", "contents": "Effects of body weight and food intake on pleasantness ratings for a sweet stimulus. A physiological role for pleasure has been suggested from observations that glucose loads reduced pleasantness ratings for sweet stimuli in normal weight but not in weight-reduced subjects. This hypothesis was reexamined in fasting and food-loaded, lean and obese subjects. Magnitude estimates of sweetness and pleasantness were obtained for seven sucrose concentrations. Indices of obesity including percent overweight, percent body fat, and body mass index were calculated. Pleasantness ratings of fasting subjects either increased up to a moderate concentration and then declined (type I hedonic response) or increased monotonically with concentration (type II hedonic response). Both types of response were found in lean and obese subjects. Within each weight group there appeared a negative correlation between pleasantness response and indices of obesity. Caloric loads reduced hedonic ratings of type II lean and obese subjects, but did not affect pleasantness response of type I lean and obese subjects. These findings indicate the presence of an hedonic monitor biased by body weight and caloric intake. The data suggest a physiological role for pleasure in regulating body weight at different set points in lean and obese subjects.", "PMID": 972136}
{"id": "pubmed23n0032_8833", "title": "Effect of angiotensin on hypoxic pulmonary vasoconstriction in isolated dog lung.", "content": "The effects of infused angiotensin on hypoxic pulmonary vasoconstriction in blood-perfused isolated dog lungs were studied. By using a double-perfusion system we were able to perfuse the right and left lungs separately in the same animal; one lung was used as control and the other lung was experimentally modified. The vasoconstrictive response to hypoxia decreased with time in the isolated lung preparations. The infusion of either angiotensin I or angiotensin II (1.2-5.8 mug/min) caused a threefold increase in the vasoconstrictive response to hypoxia over control levels. A second hypoxic period during the infusion usually yielded a diminished response, suggesting further degeneration of the response irreversible with angiotensin. It was concluded that angiotensin I or angiotensin II temporarily enhances hypoxic pulmonary vasoconstriction in isolated dog lungs.", "contents": "Effect of angiotensin on hypoxic pulmonary vasoconstriction in isolated dog lung. The effects of infused angiotensin on hypoxic pulmonary vasoconstriction in blood-perfused isolated dog lungs were studied. By using a double-perfusion system we were able to perfuse the right and left lungs separately in the same animal; one lung was used as control and the other lung was experimentally modified. The vasoconstrictive response to hypoxia decreased with time in the isolated lung preparations. The infusion of either angiotensin I or angiotensin II (1.2-5.8 mug/min) caused a threefold increase in the vasoconstrictive response to hypoxia over control levels. A second hypoxic period during the infusion usually yielded a diminished response, suggesting further degeneration of the response irreversible with angiotensin. It was concluded that angiotensin I or angiotensin II temporarily enhances hypoxic pulmonary vasoconstriction in isolated dog lungs.", "PMID": 972137}
{"id": "pubmed23n0032_8834", "title": "Effects of limping on normal exercise response.", "content": "Ten subjects performed two identical exercise tests on a motor-driven treadmill while walking normally or while limping. The two tests were separated by a rest period sufficient to permit return to a base-line state and the sugjects were randomly assigned to a normal-limp or limp-normal exercise sequence. Work rate was varied progressively by increasing treadmill grade in 5-degree increments to a maximum of 20 degrees, while limping was induced by insertion of a 0.5-cm wooden spike under the heel of the dominant foot. At all work rates, limping produced consistent increases in all variables studied, except for tidal volume where a decrease was seen. At the highest work rate these differences tended to disappear. Even considering the greater O2 consumption induced by limping, there were still significant (P less than 0.01) increases in both respiratory rate and ventilation at all but the highest work rate, accompanied by an insignificant fall in tidal volume. This suggests that these changes were not induced by the alterations in the internal or external work of walking and that limping somehow alters the regulation of exercise hyperpnea.", "contents": "Effects of limping on normal exercise response. Ten subjects performed two identical exercise tests on a motor-driven treadmill while walking normally or while limping. The two tests were separated by a rest period sufficient to permit return to a base-line state and the sugjects were randomly assigned to a normal-limp or limp-normal exercise sequence. Work rate was varied progressively by increasing treadmill grade in 5-degree increments to a maximum of 20 degrees, while limping was induced by insertion of a 0.5-cm wooden spike under the heel of the dominant foot. At all work rates, limping produced consistent increases in all variables studied, except for tidal volume where a decrease was seen. At the highest work rate these differences tended to disappear. Even considering the greater O2 consumption induced by limping, there were still significant (P less than 0.01) increases in both respiratory rate and ventilation at all but the highest work rate, accompanied by an insignificant fall in tidal volume. This suggests that these changes were not induced by the alterations in the internal or external work of walking and that limping somehow alters the regulation of exercise hyperpnea.", "PMID": 972138}
{"id": "pubmed23n0032_8835", "title": "A digital display of neuronal average firing frequencies.", "content": "A digital instrument is described which displays on a three-digit, seven-segment display the average firing frequency of a neuron. Averaging is done over essentially consecutive timing intervals of 1, 2, 5, 10, 20, or 50 s. Provision is made for overflow indication and for manual resetting. The design is simple, economical in the number of integrated circuits used, and may be adapted for measuring the average frequency of occurrence of events other than neuronal firing.", "contents": "A digital display of neuronal average firing frequencies. A digital instrument is described which displays on a three-digit, seven-segment display the average firing frequency of a neuron. Averaging is done over essentially consecutive timing intervals of 1, 2, 5, 10, 20, or 50 s. Provision is made for overflow indication and for manual resetting. The design is simple, economical in the number of integrated circuits used, and may be adapted for measuring the average frequency of occurrence of events other than neuronal firing.", "PMID": 972139}
{"id": "pubmed23n0032_8836", "title": "The response of plant mitochondria to media of high solute content.", "content": "The state-3 rate of respiration of potato tuber mitochondria is inhibited by concentrations of KCl or NaCl above 125 mM, and by concentrations of sucrose, lactose, or maltose above 500 mM, but not at all by mannitol, glucose, glycine, or proline up to a concentration of 1500 mM in the medium. Mitochondria from cauliflower, beetroot, cucumber, rock melon, and watermelon behave very similarly to those from potato tuber. The variable response to different solutes proves that the reduction in respiration is not a simple function of the chemical potential of water in the medium. Disruption of potato mitochondria by ultrasonic vibration does not relieve the inhibition of succinate oxidation caused by KCl or sucrose. However, treatment with detergent abolishes completely the inhibition of respiration by sucrose. Inhibition of succinate dehydrogenase [Succinate:PMS, oxidoreductase (EC.1.3.99.1)] and malate dehydrogenase [L-Malate:NAD oxidoreductase (EC.1.1.1.37)] activities by sucrose is less than the inhibition of succinate- and malate-dependent oxygen uptake by the potato mitochondria. Limited substrate uptake and, alternatively, reduced electron flow as a consequence of a direct effect of solute on the mitochondrial membrane are considered as possible mechanisms of inhibition.", "contents": "The response of plant mitochondria to media of high solute content. The state-3 rate of respiration of potato tuber mitochondria is inhibited by concentrations of KCl or NaCl above 125 mM, and by concentrations of sucrose, lactose, or maltose above 500 mM, but not at all by mannitol, glucose, glycine, or proline up to a concentration of 1500 mM in the medium. Mitochondria from cauliflower, beetroot, cucumber, rock melon, and watermelon behave very similarly to those from potato tuber. The variable response to different solutes proves that the reduction in respiration is not a simple function of the chemical potential of water in the medium. Disruption of potato mitochondria by ultrasonic vibration does not relieve the inhibition of succinate oxidation caused by KCl or sucrose. However, treatment with detergent abolishes completely the inhibition of respiration by sucrose. Inhibition of succinate dehydrogenase [Succinate:PMS, oxidoreductase (EC.1.3.99.1)] and malate dehydrogenase [L-Malate:NAD oxidoreductase (EC.1.1.1.37)] activities by sucrose is less than the inhibition of succinate- and malate-dependent oxygen uptake by the potato mitochondria. Limited substrate uptake and, alternatively, reduced electron flow as a consequence of a direct effect of solute on the mitochondrial membrane are considered as possible mechanisms of inhibition.", "PMID": 972140}
{"id": "pubmed23n0032_8837", "title": "Phenylenediamine restoration of photosynthetic electron flux in DBMIB-inhibited chloroplasts.", "content": "Phenylenediamines have been studied and compared as to their effectiveness in stimulating photosynthetic electron flux in DBMIB-inhibited chloroplasts. It has been found that N-substituted as well as C-substituted p-phenylenediamines accelerate the rate of ferricyanide reduction, a photosystem II photoreaction, under conditions where the radical cations of N-substituted p-phenylenediamines are stable. The P/e2 ratios for these partial reactions are between 0.4 and 0.5; this is taken as evidence that N-substituted p-phenylenediamines are reduced by the chloroplasts close to the outer surface. Both N- and C-substituted p-phenylenediamines are capable of bypassing the site of DBMIB inhibition and restoring electron flow from water to methylviologen. N-substituted p-phenylenediamines appear to be more effective even at high concentrations of DBMIB. The P/e2 ratios for these reactions are on the order of 0.75-1.0; this is taken as evidence that the bypass reaction for N-substituted p-phenylenediamines occurs on the inside of the thylakoid membrane.", "contents": "Phenylenediamine restoration of photosynthetic electron flux in DBMIB-inhibited chloroplasts. Phenylenediamines have been studied and compared as to their effectiveness in stimulating photosynthetic electron flux in DBMIB-inhibited chloroplasts. It has been found that N-substituted as well as C-substituted p-phenylenediamines accelerate the rate of ferricyanide reduction, a photosystem II photoreaction, under conditions where the radical cations of N-substituted p-phenylenediamines are stable. The P/e2 ratios for these partial reactions are between 0.4 and 0.5; this is taken as evidence that N-substituted p-phenylenediamines are reduced by the chloroplasts close to the outer surface. Both N- and C-substituted p-phenylenediamines are capable of bypassing the site of DBMIB inhibition and restoring electron flow from water to methylviologen. N-substituted p-phenylenediamines appear to be more effective even at high concentrations of DBMIB. The P/e2 ratios for these reactions are on the order of 0.75-1.0; this is taken as evidence that the bypass reaction for N-substituted p-phenylenediamines occurs on the inside of the thylakoid membrane.", "PMID": 972141}
{"id": "pubmed23n0032_8838", "title": "Inhibition of phosphoribosylpyrophosphate synthesis by purine nucleosides in human erythrocytes.", "content": "The effects of purine nucleosides on 5-phosphoribosylpyrophosphate (PP-ribose-P) synthesis were investigated in human erythrocytes in vitro. Previous observations have shown an increase of PP-ribose-P formation with nucleoside when inorganic phosphate (Pi) exceeded 20 mM. In contrast, when external Pi varied from 0 to 25 mM adenosine, inosine, guanosine, or 6-methylmercaptopurine riboside 1.25 mM decreased intracellular PP-ribose-P. The mechanism of this nucleoside effect on P-ribose-P concentration was investigated. Decreased synthesis rather than increased utilization accounted for the nucleoside effect. A decrease of erythrocyte Pi from the control values of 0.5 to 2.4 mM accompanied the nucleoside-related diminution of PP-ribose-P synthesis. This reduction of Pi was capable of decreasing the activity of PP-ribose-P synthetase which is known to be sensitive to small changes of Pi. The decrease of erythrocyte Pi levels was caused both by those nucleosides which were eventually degraded via purine nucleoside phosphorylase (adenosine, inosine, and guanosine) and by those nucleosides which were substantially phosphorylated initially by adenosine kinase (methylmercaptopurine riboside and adenosine with erythro-9(2-hydroxyl-3-nonyl)-adenine). Twenty-five per cent of methylmercaptopurine riboside was converted to the monophosphate derivative during the incubation. The presence of this compound, capable of inhibiting PP-ribose-P synthetase, provided evidence for a second mechanism of inhibition of PP-ribose-P synthesis by methylmercaptopurine riboside. No substantial increase of AMP, ADP, 2,3-diphosphoglycerate, or cyclic AMP nor a decrease of ribose 5-phosphate was found. These observations suggest that under physiological Pi concentrations nucleosides diminish PP-ribose-P synthesis mainly by decreasing intracellular Pi. Regulation of PP-ribose-P formation by alterations of intracellular Pi levels may be an important control mechanism and may account for a number of biological effects of nucleosides.", "contents": "Inhibition of phosphoribosylpyrophosphate synthesis by purine nucleosides in human erythrocytes. The effects of purine nucleosides on 5-phosphoribosylpyrophosphate (PP-ribose-P) synthesis were investigated in human erythrocytes in vitro. Previous observations have shown an increase of PP-ribose-P formation with nucleoside when inorganic phosphate (Pi) exceeded 20 mM. In contrast, when external Pi varied from 0 to 25 mM adenosine, inosine, guanosine, or 6-methylmercaptopurine riboside 1.25 mM decreased intracellular PP-ribose-P. The mechanism of this nucleoside effect on P-ribose-P concentration was investigated. Decreased synthesis rather than increased utilization accounted for the nucleoside effect. A decrease of erythrocyte Pi from the control values of 0.5 to 2.4 mM accompanied the nucleoside-related diminution of PP-ribose-P synthesis. This reduction of Pi was capable of decreasing the activity of PP-ribose-P synthetase which is known to be sensitive to small changes of Pi. The decrease of erythrocyte Pi levels was caused both by those nucleosides which were eventually degraded via purine nucleoside phosphorylase (adenosine, inosine, and guanosine) and by those nucleosides which were substantially phosphorylated initially by adenosine kinase (methylmercaptopurine riboside and adenosine with erythro-9(2-hydroxyl-3-nonyl)-adenine). Twenty-five per cent of methylmercaptopurine riboside was converted to the monophosphate derivative during the incubation. The presence of this compound, capable of inhibiting PP-ribose-P synthetase, provided evidence for a second mechanism of inhibition of PP-ribose-P synthesis by methylmercaptopurine riboside. No substantial increase of AMP, ADP, 2,3-diphosphoglycerate, or cyclic AMP nor a decrease of ribose 5-phosphate was found. These observations suggest that under physiological Pi concentrations nucleosides diminish PP-ribose-P synthesis mainly by decreasing intracellular Pi. Regulation of PP-ribose-P formation by alterations of intracellular Pi levels may be an important control mechanism and may account for a number of biological effects of nucleosides.", "PMID": 972142}
{"id": "pubmed23n0032_8839", "title": "Determination of the subunit composition of haptoglobin 2-1 polymers using quantitative densitometry of polyacrylamide gels.", "content": "Human haptoglobin (Hp), a hemoglobin-binding glycoprotein containing two types of polypeptide chains, alpha and beta, in equimolar amounts linked by disulfide bonds, exists in three major phenotypes determined by the properties of the alpha chain: Hp 1-1 (alpha1), Hp 2-2 (alpha2), and Hp 2-1 (alpha1 and alpha2). Hp 2-2 and Hp 2-1 form a series of alpha-disulfide-linked polymers. The subunit composition of the Hp 2-1 series was studied by isolation of single Hp 2-1 polymers by polyacrylamide gel electrophoresis. After reductive disulfide cleavage and alkylation the relative content of alpha2 and alpha1 polypeptide chains was determined by quantitative densitometry of acid/urea polyacrylamide gels stained with Coomassie brilliant blue R250. The molar ratios alpha2/alpha1 for the Hp 2-1 polymers. P1 through P5 (in order of decreasing electrophoretic mobility), were found to be: P1, 0.0 (alpha1 only); P2, 0.48; P3, 0.97; P4, 1.6; P5, 2.0. Since one alphabeta-Hb half-molecule is known to bind to each Hp beta chain, the beta polypeptide chain content of each of the Hp 2-1 polymers could be estimated by by counting the number of Coomassie blue bands formed after electrophoresis of isolated Hp 2-1 polymers fractionally saturated with cyanmethemoglobin (Hb). The number of beta chains present in Hp 2-1 polymers P1 through P4 was determined to be: P1, 2; P2, 3; P3, 4 and P4, 5. Molecular weights of the Hp 2-1 polymers were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresus using as standards the almost homologous Hp 2-2 polymer series whose molecular weights are known from ultracentrifugation studies. Molecular weights for the first five Hp 2-1 polymers were estimated to be 107,000; 162,000; 217,000, 274,000; and 331,000, respectively. These data are consistent with the previously proposed model for the subunit composition of the Hp 2-1 polymer series when P1 = (alpha1 beta)2 and the subsequent polymers in order are represented as (alpha1 beta)2(alpha2 beta)n where n = 1,2,3,4...", "contents": "Determination of the subunit composition of haptoglobin 2-1 polymers using quantitative densitometry of polyacrylamide gels. Human haptoglobin (Hp), a hemoglobin-binding glycoprotein containing two types of polypeptide chains, alpha and beta, in equimolar amounts linked by disulfide bonds, exists in three major phenotypes determined by the properties of the alpha chain: Hp 1-1 (alpha1), Hp 2-2 (alpha2), and Hp 2-1 (alpha1 and alpha2). Hp 2-2 and Hp 2-1 form a series of alpha-disulfide-linked polymers. The subunit composition of the Hp 2-1 series was studied by isolation of single Hp 2-1 polymers by polyacrylamide gel electrophoresis. After reductive disulfide cleavage and alkylation the relative content of alpha2 and alpha1 polypeptide chains was determined by quantitative densitometry of acid/urea polyacrylamide gels stained with Coomassie brilliant blue R250. The molar ratios alpha2/alpha1 for the Hp 2-1 polymers. P1 through P5 (in order of decreasing electrophoretic mobility), were found to be: P1, 0.0 (alpha1 only); P2, 0.48; P3, 0.97; P4, 1.6; P5, 2.0. Since one alphabeta-Hb half-molecule is known to bind to each Hp beta chain, the beta polypeptide chain content of each of the Hp 2-1 polymers could be estimated by by counting the number of Coomassie blue bands formed after electrophoresis of isolated Hp 2-1 polymers fractionally saturated with cyanmethemoglobin (Hb). The number of beta chains present in Hp 2-1 polymers P1 through P4 was determined to be: P1, 2; P2, 3; P3, 4 and P4, 5. Molecular weights of the Hp 2-1 polymers were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresus using as standards the almost homologous Hp 2-2 polymer series whose molecular weights are known from ultracentrifugation studies. Molecular weights for the first five Hp 2-1 polymers were estimated to be 107,000; 162,000; 217,000, 274,000; and 331,000, respectively. These data are consistent with the previously proposed model for the subunit composition of the Hp 2-1 polymer series when P1 = (alpha1 beta)2 and the subsequent polymers in order are represented as (alpha1 beta)2(alpha2 beta)n where n = 1,2,3,4...", "PMID": 972143}
{"id": "pubmed23n0032_8840", "title": "Structure of the accumulating oligosaccharide in fucosidosis.", "content": "Two water-soluble complex carbohydrate storage products were isolated from tissues and urine of patients with an inherited deficiency of lysosomal alpha-L-fucosidase (fucosidosis). The major component was an oligosaccharide of approximate molecular weight 1700, indicating that it was a dekasaccharide. From a combination of sequential digestion with purified exo-glycosidases, periodate oxidation and permethylation in conjunction with gas-liquid chromatography mass spectrometric analysis, the structure was found to be: Fuc(alpha 1 leads to 2)Gal-(beta 1 leads to 4) GlcNAc (beta1 leads to 2)Man [Fuc(alpha1 leads to 2) Gal (beta1 leads to 4) GlcNAc(beta1 leads to 2) Man] (alpha 1 leads to 3/6) Man (beta1 leads to 4) GlcNAc, although there was some evidence for heterogeneity at the mannose branchpoint. This material is structurally related to the stored oligosaccharides in patients with inherited deficiencies of beta-galactosidase (G M1-gangliosidosis) and N-acetyl-beta-hexosaminidase (G M2-gangliosidosis). A dissaccharide with the probable structure Fuc(alpha1 leads to 6)GlcNAc was found in lesser amounts in tissues; both are believed to be derived from the impaired catabolism of large numbers of different glycoproteins.", "contents": "Structure of the accumulating oligosaccharide in fucosidosis. Two water-soluble complex carbohydrate storage products were isolated from tissues and urine of patients with an inherited deficiency of lysosomal alpha-L-fucosidase (fucosidosis). The major component was an oligosaccharide of approximate molecular weight 1700, indicating that it was a dekasaccharide. From a combination of sequential digestion with purified exo-glycosidases, periodate oxidation and permethylation in conjunction with gas-liquid chromatography mass spectrometric analysis, the structure was found to be: Fuc(alpha 1 leads to 2)Gal-(beta 1 leads to 4) GlcNAc (beta1 leads to 2)Man [Fuc(alpha1 leads to 2) Gal (beta1 leads to 4) GlcNAc(beta1 leads to 2) Man] (alpha 1 leads to 3/6) Man (beta1 leads to 4) GlcNAc, although there was some evidence for heterogeneity at the mannose branchpoint. This material is structurally related to the stored oligosaccharides in patients with inherited deficiencies of beta-galactosidase (G M1-gangliosidosis) and N-acetyl-beta-hexosaminidase (G M2-gangliosidosis). A dissaccharide with the probable structure Fuc(alpha1 leads to 6)GlcNAc was found in lesser amounts in tissues; both are believed to be derived from the impaired catabolism of large numbers of different glycoproteins.", "PMID": 972144}
{"id": "pubmed23n0032_8841", "title": "Concanavalin A binding to membranes of the Golgi apparatus and resultant modification of galactosyltransferase activity.", "content": "125I-Concanavalin A was found to bind to purified Golgi membranes in a manner which was time-dependent, proportional to the amount of Golgi protein, saturable, and inhibited by alpha-methyl-D-glucoside and unlabeled concanavalin A. Approximately 2 nmol of 125I-concanavalin A were bound per mg of membrane protein with a mean Ka of 0.2 x 10(7) M-1. After binding of concanavalin A to the membranes, all unbound lectin was removed by repeated washing prior to assay of galactosyltransferase activity to assure that bulk concanavalin A would not bind to the sugar acceptor during the enzyme assay. Therefore, the effects of the lectin were not due to altered acceptor concentrations in the assay. As a result of concanavalin A binding the activity of the galactosyltransferase of these membranes was enhanced by 70 to 100%. The enhancement was abolished by treatment of the concanavalin A-bound Golgi with alpha-methylglucoside. Kinetically this stimulation was reflected as an altered Km for N-acetylglucosamine and UDP-galactose. The data are suggestive that the stimulation of the enzyme results indirectly from perturbation of the membranes on binding of the lectin.", "contents": "Concanavalin A binding to membranes of the Golgi apparatus and resultant modification of galactosyltransferase activity. 125I-Concanavalin A was found to bind to purified Golgi membranes in a manner which was time-dependent, proportional to the amount of Golgi protein, saturable, and inhibited by alpha-methyl-D-glucoside and unlabeled concanavalin A. Approximately 2 nmol of 125I-concanavalin A were bound per mg of membrane protein with a mean Ka of 0.2 x 10(7) M-1. After binding of concanavalin A to the membranes, all unbound lectin was removed by repeated washing prior to assay of galactosyltransferase activity to assure that bulk concanavalin A would not bind to the sugar acceptor during the enzyme assay. Therefore, the effects of the lectin were not due to altered acceptor concentrations in the assay. As a result of concanavalin A binding the activity of the galactosyltransferase of these membranes was enhanced by 70 to 100%. The enhancement was abolished by treatment of the concanavalin A-bound Golgi with alpha-methylglucoside. Kinetically this stimulation was reflected as an altered Km for N-acetylglucosamine and UDP-galactose. The data are suggestive that the stimulation of the enzyme results indirectly from perturbation of the membranes on binding of the lectin.", "PMID": 972145}
{"id": "pubmed23n0032_8842", "title": "Poly (A)-rich ribonucleoprotein complexes from HeLa cell messenger RNA.", "content": "Polyribosomal messenger RNA from HeLa cells contain 3'-OH-terminal polyadenylate sequences approximately 133 nucleotides in length (weight average). When analyzed at the ribonucleoprotein level of organization these poly(A)-rich sequences are found to contain tightly bound proteins. These proteins remain associated with the poly(A)-rich RNA during affinity chromatography of RNase A and T1-digested polyribosomes on poly(U)-Sepharose in 0.5 M NaCl, and co-elute from the column with the RNA at 50% formamide. Controls establish that the co-purification of the proteins with poly(A) on poly(U)-Sepharose requires the molecular integrity of the poly(A). Polyacrylamide gel electrophoresis resolves the poly(A)-specific proteins into two components of 74,000 and 62,000 molecular weight. The larger protein is the same size as that previously reported to be associated with poly(A)-rich sequences in HeLa heterogeneous nuclear RNA (Kish, V.M., and Pederson, T. (1975), J. Mol. Biol. 95, 227-238). It is concluded that both HeLa nuclear and polyribosomal poly(A) sequences have a protein (62,000 molecular weight) associated with poly(A) appears to be confined only to messenger RNA.", "contents": "Poly (A)-rich ribonucleoprotein complexes from HeLa cell messenger RNA. Polyribosomal messenger RNA from HeLa cells contain 3'-OH-terminal polyadenylate sequences approximately 133 nucleotides in length (weight average). When analyzed at the ribonucleoprotein level of organization these poly(A)-rich sequences are found to contain tightly bound proteins. These proteins remain associated with the poly(A)-rich RNA during affinity chromatography of RNase A and T1-digested polyribosomes on poly(U)-Sepharose in 0.5 M NaCl, and co-elute from the column with the RNA at 50% formamide. Controls establish that the co-purification of the proteins with poly(A) on poly(U)-Sepharose requires the molecular integrity of the poly(A). Polyacrylamide gel electrophoresis resolves the poly(A)-specific proteins into two components of 74,000 and 62,000 molecular weight. The larger protein is the same size as that previously reported to be associated with poly(A)-rich sequences in HeLa heterogeneous nuclear RNA (Kish, V.M., and Pederson, T. (1975), J. Mol. Biol. 95, 227-238). It is concluded that both HeLa nuclear and polyribosomal poly(A) sequences have a protein (62,000 molecular weight) associated with poly(A) appears to be confined only to messenger RNA.", "PMID": 972146}
{"id": "pubmed23n0032_8843", "title": "The NH2- and COOH-terminal amino acid sequence of nuclear protein A24.", "content": "The NH2- and COOH-terminal sequence of nuclear portein A24 has been determined by automatic Edman degradation and carboxypeptidase A and B digestion. Protein A24 is of interest because it is composed in part of histone 2A (Goldknofp, I.L., and Busch, H., (1975) Biochem, Biophys. Res. Commun. 65, 951-960). The sequence of the first 37 NH2-terminal residues is: Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly-Lys-Thr-Ile-Thr-Leu-Glu-Val-Glu-Pro-Ser-Asp-Thr-Ile-Glu-Asn-Val-Lys-Ala-Lys-Ile-Gln-Asp-Lys-Glu-Gly-Ile-Pro- This sequence is not homologous to any known histone sequence. It contains regions of internal homology (italics). The COOH-terminal amino acid sequence is the same as that of histone 2A, naely: -His-His-Lys-Ala-Lys-Gly-Lys-COOH.", "contents": "The NH2- and COOH-terminal amino acid sequence of nuclear protein A24. The NH2- and COOH-terminal sequence of nuclear portein A24 has been determined by automatic Edman degradation and carboxypeptidase A and B digestion. Protein A24 is of interest because it is composed in part of histone 2A (Goldknofp, I.L., and Busch, H., (1975) Biochem, Biophys. Res. Commun. 65, 951-960). The sequence of the first 37 NH2-terminal residues is: Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly-Lys-Thr-Ile-Thr-Leu-Glu-Val-Glu-Pro-Ser-Asp-Thr-Ile-Glu-Asn-Val-Lys-Ala-Lys-Ile-Gln-Asp-Lys-Glu-Gly-Ile-Pro- This sequence is not homologous to any known histone sequence. It contains regions of internal homology (italics). The COOH-terminal amino acid sequence is the same as that of histone 2A, naely: -His-His-Lys-Ala-Lys-Gly-Lys-COOH.", "PMID": 972147}
{"id": "pubmed23n0032_8844", "title": "Purification and characterization of two lectins from Caragana arborescens seeds.", "content": "A glycoprotein fraction with hemagglutinating activity was purified by affinity chromatography from seeds of the pea tree, Caragana arborescens. Subsequent fractionation resolved two components, which could be separated on a preparative scale using different affinity matrices. The major component binds to N-acetylgalactosamine coupled to Sepharose 4B. It is a glycoprotein with high hemagglutinating activity. It is composed of two types of polypeptides, present in nonstoichiometric amounts, with apparent molecular weights near 30,000. In the native molecule, the subunits are cross-linked by disulfide bonds to form dimers, which in turn appear to be in rapid equilibrium with tetramers. The minor component binds to underivatized Sepharose 4B. It too, is a glycoprotein but has low hemagglutinating activity. It is composed of three types of polypeptides which, although they have apparent molecular weights near 30,000 are distinguished from the subunits of the major hemagglutinin by a number of physical and chemical properties. The native molecule is dimeric, with a mass of 60,000 daltons. The major component has high affinity (K = 0.1 mM) for the haptenic sugar, N-acetylgalactosamine, but will also bind D-galactose. Neither lectin has ABO blood group specificity, nor are they transformed mouse fibroblasts to the same extent.", "contents": "Purification and characterization of two lectins from Caragana arborescens seeds. A glycoprotein fraction with hemagglutinating activity was purified by affinity chromatography from seeds of the pea tree, Caragana arborescens. Subsequent fractionation resolved two components, which could be separated on a preparative scale using different affinity matrices. The major component binds to N-acetylgalactosamine coupled to Sepharose 4B. It is a glycoprotein with high hemagglutinating activity. It is composed of two types of polypeptides, present in nonstoichiometric amounts, with apparent molecular weights near 30,000. In the native molecule, the subunits are cross-linked by disulfide bonds to form dimers, which in turn appear to be in rapid equilibrium with tetramers. The minor component binds to underivatized Sepharose 4B. It too, is a glycoprotein but has low hemagglutinating activity. It is composed of three types of polypeptides which, although they have apparent molecular weights near 30,000 are distinguished from the subunits of the major hemagglutinin by a number of physical and chemical properties. The native molecule is dimeric, with a mass of 60,000 daltons. The major component has high affinity (K = 0.1 mM) for the haptenic sugar, N-acetylgalactosamine, but will also bind D-galactose. Neither lectin has ABO blood group specificity, nor are they transformed mouse fibroblasts to the same extent.", "PMID": 972148}
{"id": "pubmed23n0032_8845", "title": "Electronic structure and bonding of the amino acids containing first row atoms.", "content": "The electronic structures of the amino acids containing first row atoms have been determined for the zwitterionic form using an approximate self-consistent field method, partial retention of diatomic differential overlap. Various energetic quantities including certain proton affinities are present as are eigenvalues for the highest occupied and lowest unoccupied molecular orbitals. It is found that our method, in common with all methods employing minimum basis sets, yields eigenvalues for the highest occupied molecular orbital that are too high. The method does predict the location of this orbital correctly when compared to calculations employing larger basis sets. It is predicted that electron loss due to ionizing radiation should occur from the carboxylate group for the nonaromatic amino acids, while for tyrosine and tryptophan, electron loss should occur from the ring system. No choice between these two sites can be made for phenylalanine. Charge distributions have been obtained which show that only partial zwitterionic character is found in the backbone and that little delocalization of charge from the backbone to the side chain occurs. Localized molecular orbitals have been obtained using the Boys criteria and the bonding in the amino acids is disscussed in terms of these orbitals. Hybridization of various bonds and bond polarities are discussed as is the phenomenon of fractional bonding to carbon.", "contents": "Electronic structure and bonding of the amino acids containing first row atoms. The electronic structures of the amino acids containing first row atoms have been determined for the zwitterionic form using an approximate self-consistent field method, partial retention of diatomic differential overlap. Various energetic quantities including certain proton affinities are present as are eigenvalues for the highest occupied and lowest unoccupied molecular orbitals. It is found that our method, in common with all methods employing minimum basis sets, yields eigenvalues for the highest occupied molecular orbital that are too high. The method does predict the location of this orbital correctly when compared to calculations employing larger basis sets. It is predicted that electron loss due to ionizing radiation should occur from the carboxylate group for the nonaromatic amino acids, while for tyrosine and tryptophan, electron loss should occur from the ring system. No choice between these two sites can be made for phenylalanine. Charge distributions have been obtained which show that only partial zwitterionic character is found in the backbone and that little delocalization of charge from the backbone to the side chain occurs. Localized molecular orbitals have been obtained using the Boys criteria and the bonding in the amino acids is disscussed in terms of these orbitals. Hybridization of various bonds and bond polarities are discussed as is the phenomenon of fractional bonding to carbon.", "PMID": 972149}
{"id": "pubmed23n0032_8846", "title": "Maternal-fetal carnitine relationship and neonatal ketosis in the rat.", "content": "The concentration of ketone bodies in plasma and of carnitine in various maternal, fetal, and neonatal tissues was examined during the developmental period in rats. Plasma ketone levels were low in the fetus, increased 10-fold during the first 24 h postpartom, and thereafter gradually declined such that normal values were found at the end of the suckling period. An almost identical profile was observed for liver carnitine concentrations in the baby rats. The converse was true for heart tissue, the carnitine content of which was low at birth and steadily increased to adult levels with the time of suckling. The primary source of carnitine in neonatal tissues, at least during the first 2 to 3 days postpartum, was shown to be the mother rat whose liver and milk carnitine content was very high at this time and fell as nursing continued. Experiments in which the fate of [14C]butyrobetaine, the immediate precursor of carnitine, was followed after injection into nursing mother rats indicated movement of carnitine from maternal liver leads to maternal plasma leads to milk leads to neonatal tissues. The above findings support the view expressed earlier that one prerequisite for the development of a high ketogenic profile in liver may be an elevation in the tissue carnitine concentration. Additional factors, however, are clearly involved as evidenced by the observation that in the fed state perfused livers from nursing mother rats synthesized ketone bodies from oleic acid at low rates compared with those seen after a 24 h fast, despite the fact that tissue carnitine levels were equally elevated in both groups. This paradox is likely related to the fact that in the fed state such livers also contained large quantities of glycogen, depletion of which through fasting was accompanied by marked acceleration of ketogenesis from oleate. The data indicate, therefore, that maximal ketogenic capacity of the liver requires for its induction an increase in carnitine coupled with a decrease in glycogen content of the tissue.", "contents": "Maternal-fetal carnitine relationship and neonatal ketosis in the rat. The concentration of ketone bodies in plasma and of carnitine in various maternal, fetal, and neonatal tissues was examined during the developmental period in rats. Plasma ketone levels were low in the fetus, increased 10-fold during the first 24 h postpartom, and thereafter gradually declined such that normal values were found at the end of the suckling period. An almost identical profile was observed for liver carnitine concentrations in the baby rats. The converse was true for heart tissue, the carnitine content of which was low at birth and steadily increased to adult levels with the time of suckling. The primary source of carnitine in neonatal tissues, at least during the first 2 to 3 days postpartum, was shown to be the mother rat whose liver and milk carnitine content was very high at this time and fell as nursing continued. Experiments in which the fate of [14C]butyrobetaine, the immediate precursor of carnitine, was followed after injection into nursing mother rats indicated movement of carnitine from maternal liver leads to maternal plasma leads to milk leads to neonatal tissues. The above findings support the view expressed earlier that one prerequisite for the development of a high ketogenic profile in liver may be an elevation in the tissue carnitine concentration. Additional factors, however, are clearly involved as evidenced by the observation that in the fed state perfused livers from nursing mother rats synthesized ketone bodies from oleic acid at low rates compared with those seen after a 24 h fast, despite the fact that tissue carnitine levels were equally elevated in both groups. This paradox is likely related to the fact that in the fed state such livers also contained large quantities of glycogen, depletion of which through fasting was accompanied by marked acceleration of ketogenesis from oleate. The data indicate, therefore, that maximal ketogenic capacity of the liver requires for its induction an increase in carnitine coupled with a decrease in glycogen content of the tissue.", "PMID": 972150}
{"id": "pubmed23n0032_8847", "title": "Effect of glycosylation on the in vivo circulating half-life of ribonuclease.", "content": "The circulating half-lives of the four isozymes of bovine pancreatic ribonuclease (RNases A, B, C, and D) have been determined in normal and in nephrectomized rats. The isozymes differ only in their glycosyl content. While A contains no sugars, B has a simple oligosaccharide (GlcNAc2 Man4-5),and C and D each have a complex oligosaccharide (GlcNAc4 Man 2-3 Gal2 Fuc NeuAc2, and GlcNAc4 Man3 Gal2 Fuc NeuAc4, respectively) attached to Asn-34 of the polypeptide chain. All four isozymes were cleared rapidly in normal rats (t 1/2 = 2 to 3 min), as expected on the basis of the established role of the kidneys in removing low molecular weight proteins from circulation. In nephrectomized rats, however, a much slower clearance was observed, thus permitting the evaluation of the role of the carbohydrate chains in the catabolism of the isozymes. The clearance curves can be analyzed in terms of two processes, a rapid initial one, shown to represent the equilibration of the injected enzyme into extravascular space, and a second one which is interpreted as the catabolic clearance of the enzyme. The haf-life of the RNase isozymes was calculated from this second process and found to be in the range 528 to 577 min for RNase A, 15 min for RNase B, 681 to 862 min for RNase C, and 839 to 941 min for RNase D. The rapidly cleared RNase B was treated with alpha-mannosidase to remove 3 of the 4 mannosyl residues, leaving only a trisaccharide (GlcNAc2-betaMan) attached to the protein. The half-life of this RNase B derivatives was found to be in the range 616 to 733 min. From these results it is concluded (a) that the addition of complex oligosaccharides to a protein does not have any significant direct effect on its circulating half-life (RNases C and D compared to RNase A), and (b) that in the rat there exists a mechanism for clearing glycoproteins based on specific recognition of exposed alpha-mannosyl residues (RNase B compared to the other isozymes and to alpha-mannosidase-treated RNase B).", "contents": "Effect of glycosylation on the in vivo circulating half-life of ribonuclease. The circulating half-lives of the four isozymes of bovine pancreatic ribonuclease (RNases A, B, C, and D) have been determined in normal and in nephrectomized rats. The isozymes differ only in their glycosyl content. While A contains no sugars, B has a simple oligosaccharide (GlcNAc2 Man4-5),and C and D each have a complex oligosaccharide (GlcNAc4 Man 2-3 Gal2 Fuc NeuAc2, and GlcNAc4 Man3 Gal2 Fuc NeuAc4, respectively) attached to Asn-34 of the polypeptide chain. All four isozymes were cleared rapidly in normal rats (t 1/2 = 2 to 3 min), as expected on the basis of the established role of the kidneys in removing low molecular weight proteins from circulation. In nephrectomized rats, however, a much slower clearance was observed, thus permitting the evaluation of the role of the carbohydrate chains in the catabolism of the isozymes. The clearance curves can be analyzed in terms of two processes, a rapid initial one, shown to represent the equilibration of the injected enzyme into extravascular space, and a second one which is interpreted as the catabolic clearance of the enzyme. The haf-life of the RNase isozymes was calculated from this second process and found to be in the range 528 to 577 min for RNase A, 15 min for RNase B, 681 to 862 min for RNase C, and 839 to 941 min for RNase D. The rapidly cleared RNase B was treated with alpha-mannosidase to remove 3 of the 4 mannosyl residues, leaving only a trisaccharide (GlcNAc2-betaMan) attached to the protein. The half-life of this RNase B derivatives was found to be in the range 616 to 733 min. From these results it is concluded (a) that the addition of complex oligosaccharides to a protein does not have any significant direct effect on its circulating half-life (RNases C and D compared to RNase A), and (b) that in the rat there exists a mechanism for clearing glycoproteins based on specific recognition of exposed alpha-mannosyl residues (RNase B compared to the other isozymes and to alpha-mannosidase-treated RNase B).", "PMID": 972151}
{"id": "pubmed23n0032_8848", "title": "Structure and action of heteronemertine polypeptide toxins. Primary structure of Cerebratulus lacteus toxin B-IV.", "content": "The amino acid sequence of Cerebratulus toxin B-IV, a crustacean-selective axonal toxin occurring in the marine worm C. lacteus, was determined by Edman degradation of the tryptic and staphylococcal protease peptides obtained from the reduced, carboxymethylated toxin. All four of the anticipated maleylated tryptic peptides, ranging in size from 8 to 23 residues, and three staphylococcal protease peptides, ranging from 9 to 35 residues, were isolated in pure form by gel filtration followed by either ion exchange chromatography or preparative paper electrophoresis. The order of the maleylated tryptic peptides was based upon the sequences of the staphylococcal protease peptides. As might be expected, toxin B-IV displays no homology with the elapid nicotinic receptor toxins. In addition, toxin B-IV is structurally unrelated to a group of scorpion neurotoxins which, like B-IV, affect action potential generating mechanisms.", "contents": "Structure and action of heteronemertine polypeptide toxins. Primary structure of Cerebratulus lacteus toxin B-IV. The amino acid sequence of Cerebratulus toxin B-IV, a crustacean-selective axonal toxin occurring in the marine worm C. lacteus, was determined by Edman degradation of the tryptic and staphylococcal protease peptides obtained from the reduced, carboxymethylated toxin. All four of the anticipated maleylated tryptic peptides, ranging in size from 8 to 23 residues, and three staphylococcal protease peptides, ranging from 9 to 35 residues, were isolated in pure form by gel filtration followed by either ion exchange chromatography or preparative paper electrophoresis. The order of the maleylated tryptic peptides was based upon the sequences of the staphylococcal protease peptides. As might be expected, toxin B-IV displays no homology with the elapid nicotinic receptor toxins. In addition, toxin B-IV is structurally unrelated to a group of scorpion neurotoxins which, like B-IV, affect action potential generating mechanisms.", "PMID": 972152}
{"id": "pubmed23n0032_8849", "title": "Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the nonhelical ends in solution.", "content": "Procedures for the preparation of soluble collagen from rat skin and tail tendon were reviewed and revised to permit the preparation of native monomeric collagen with intact nonhelical ends. The degree of intactness was estimated from the tyrosine content, which is present only in the nonhelical ends, and by mobility of the COOH-terminal cyanogen bromide peptide of the alpha1 chain on sodium dodecyl sulfate gels. The amount of covalently cross-linked polymeric material present was estimated by molecular sieve chromatography of denatured samples. Rapid purification in the cold was sufficient to prevent or greatly reduce proteolytic alteration. Fractionation by salt precipitation at acid pH was effective in reducing the content of polymeric material. Rat tail tendon yielded completely intact native collagen, but some high molecular weight aggregates remained. Collagen from the skin of lathyritic rats was easier to obtain free of aggregates, but contained about 1 less tyrosine residue per alpha1 chain even when isolated in the presence of enzyme inhibitors. Proton NMR spectra of denatured acidic solutions of these preparations showed that 4 to 5 tyrosine residues per alpha chain were present, confirming the chemical analysis. Spectra of the native molecule showed that about the same number of tyrosine residues per chain are in rapid motion, unlike residues in the helical portion of the molecule, a result which shows that the nonhelical ends of the native molecule are unstructured in acidic solution.", "contents": "Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the nonhelical ends in solution. Procedures for the preparation of soluble collagen from rat skin and tail tendon were reviewed and revised to permit the preparation of native monomeric collagen with intact nonhelical ends. The degree of intactness was estimated from the tyrosine content, which is present only in the nonhelical ends, and by mobility of the COOH-terminal cyanogen bromide peptide of the alpha1 chain on sodium dodecyl sulfate gels. The amount of covalently cross-linked polymeric material present was estimated by molecular sieve chromatography of denatured samples. Rapid purification in the cold was sufficient to prevent or greatly reduce proteolytic alteration. Fractionation by salt precipitation at acid pH was effective in reducing the content of polymeric material. Rat tail tendon yielded completely intact native collagen, but some high molecular weight aggregates remained. Collagen from the skin of lathyritic rats was easier to obtain free of aggregates, but contained about 1 less tyrosine residue per alpha1 chain even when isolated in the presence of enzyme inhibitors. Proton NMR spectra of denatured acidic solutions of these preparations showed that 4 to 5 tyrosine residues per alpha chain were present, confirming the chemical analysis. Spectra of the native molecule showed that about the same number of tyrosine residues per chain are in rapid motion, unlike residues in the helical portion of the molecule, a result which shows that the nonhelical ends of the native molecule are unstructured in acidic solution.", "PMID": 972153}
{"id": "pubmed23n0032_8850", "title": "Absence of the major sialoglycoprotein in the membrane of human En(a--) erythrocytes and increased glycosylation of band 3.", "content": "The human En(a-) blood group is a rare recessive trait. These erythrocytes lack the major membrane sialoglycoprotein (PAS1, MN protein, or glycophorin) and contain a decreased amount of PAS2 as demonstrated by radiolabeling of surface porteins and chemical techniques. A third glycoprotein, Band 3, contains two labeled oligosaccharide chains; the more complex oligosaccharide has a higher molecular weight in En(1-) cells than in normal cells. A fourth glycoprotein, PAS3, is present in usual amounts in En(a-) cells. Cells heterozygous for En(a) are intermediate in these respects. The sialic acid is decreased in En(a-) cells, but the total carbohydrate is similar in the different membranes. The glycolipids are present in normal amounts but are much more exposed to galactose oxidase in En(a-) cells than in normal cells.", "contents": "Absence of the major sialoglycoprotein in the membrane of human En(a--) erythrocytes and increased glycosylation of band 3. The human En(a-) blood group is a rare recessive trait. These erythrocytes lack the major membrane sialoglycoprotein (PAS1, MN protein, or glycophorin) and contain a decreased amount of PAS2 as demonstrated by radiolabeling of surface porteins and chemical techniques. A third glycoprotein, Band 3, contains two labeled oligosaccharide chains; the more complex oligosaccharide has a higher molecular weight in En(1-) cells than in normal cells. A fourth glycoprotein, PAS3, is present in usual amounts in En(a-) cells. Cells heterozygous for En(a) are intermediate in these respects. The sialic acid is decreased in En(a-) cells, but the total carbohydrate is similar in the different membranes. The glycolipids are present in normal amounts but are much more exposed to galactose oxidase in En(a-) cells than in normal cells.", "PMID": 972154}
{"id": "pubmed23n0032_8851", "title": "Deuterium magnetic resonance spectroscopy of isotopically labeled  mammalian cells.", "content": "Choline completely deuterated in the methyl groups has been incorporated into two mammalian cell systems. The first was the chemically transformed mouse fibroblast LM cell line, grown in suspension culture. The second system consisted of mitochondria from Sprague-Dawley rats which were fed upon a choline-deficient diet enriched with deuterated choline chloride. Two classes of deuterium nuclear magnetic resonance (NMR) signal were obtained from each system. The first class of signal exhibited no residual quadrupole coupling constant and was assigned to the natural abundance of 2H in water, together with free labeled choline. The second class of signal had a residual quadrupole coupling of about 1 HKz and was assigned to 2H-labeled choline headgroups of phospholipids. These results are in conflict with a previous study of deuterated rat liver mitochondrial membranes. Deuterium magnetic resonance spectra can be obtained on mammalian membranes that have incorporated specific deuterium-labeled compounds and, therefore, provides a very powerful method for studying the dynamic structure of membranes.", "contents": "Deuterium magnetic resonance spectroscopy of isotopically labeled  mammalian cells. Choline completely deuterated in the methyl groups has been incorporated into two mammalian cell systems. The first was the chemically transformed mouse fibroblast LM cell line, grown in suspension culture. The second system consisted of mitochondria from Sprague-Dawley rats which were fed upon a choline-deficient diet enriched with deuterated choline chloride. Two classes of deuterium nuclear magnetic resonance (NMR) signal were obtained from each system. The first class of signal exhibited no residual quadrupole coupling constant and was assigned to the natural abundance of 2H in water, together with free labeled choline. The second class of signal had a residual quadrupole coupling of about 1 HKz and was assigned to 2H-labeled choline headgroups of phospholipids. These results are in conflict with a previous study of deuterated rat liver mitochondrial membranes. Deuterium magnetic resonance spectra can be obtained on mammalian membranes that have incorporated specific deuterium-labeled compounds and, therefore, provides a very powerful method for studying the dynamic structure of membranes.", "PMID": 972155}
{"id": "pubmed23n0032_8852", "title": "The repair of the surface structure of animal cells.", "content": "Experiments were performed to determine if animal cells in culture possess specific mechanisms to repair surface molecules damaged by enzymes. The surface membranes of a primary cell culture, chick fibroblasts, a permanent hamster cell line, BHK21/C13, and its virally transformed counterpart, C13/B4 were damaged by exposure to trypsin or to neuraminidase. Following digestion with trypsin, the incorporation of radioactive amino acids or sugars into purified surface membrane of cells was monitored. No differences were noted in rates of incorporation when control and trypsin-damaged cells were compared. Neuraminidase damage to the surface of BHK21/C13 and C13/B4 cells was evidenced by altered gel filtration profiles of surface glycopeptides, i.e., delayed elution because of reduction in size. By labelling cells with 14C-L-fucose prior to neuraminidase treatment and following the incorporation of 3H-L-fucose into cell surface glycopeptides after neuraminidase digestion, we were able to monitor the synthesis and turnover of fucose-containing glycopeptides in the same cells. Gel filtration profiles indicated that little or no desialylated glycoproteins were resialylated (repaired) by specific replacement of sialic acid. Comparing neuraminidase-digested and control cells we observed no difference in rates of 3H-L-fucose incorporation or of 14C-L-fucose loss from these cells; nor did we find differences in the rate of incorporation of isotopic glucosamine into sialic acid. Neuraminidase treatment failed to alter the rate of cell growth or the pattern of isotopic incorporation into various cell surface components. These results support the suggestion that return of sialic acid (repair) was effected by turnover which serves as a non-specific repair mechanism to replace damaged cell surface molecules (Warren and Glick '68; Warren, '69).", "contents": "The repair of the surface structure of animal cells. Experiments were performed to determine if animal cells in culture possess specific mechanisms to repair surface molecules damaged by enzymes. The surface membranes of a primary cell culture, chick fibroblasts, a permanent hamster cell line, BHK21/C13, and its virally transformed counterpart, C13/B4 were damaged by exposure to trypsin or to neuraminidase. Following digestion with trypsin, the incorporation of radioactive amino acids or sugars into purified surface membrane of cells was monitored. No differences were noted in rates of incorporation when control and trypsin-damaged cells were compared. Neuraminidase damage to the surface of BHK21/C13 and C13/B4 cells was evidenced by altered gel filtration profiles of surface glycopeptides, i.e., delayed elution because of reduction in size. By labelling cells with 14C-L-fucose prior to neuraminidase treatment and following the incorporation of 3H-L-fucose into cell surface glycopeptides after neuraminidase digestion, we were able to monitor the synthesis and turnover of fucose-containing glycopeptides in the same cells. Gel filtration profiles indicated that little or no desialylated glycoproteins were resialylated (repaired) by specific replacement of sialic acid. Comparing neuraminidase-digested and control cells we observed no difference in rates of 3H-L-fucose incorporation or of 14C-L-fucose loss from these cells; nor did we find differences in the rate of incorporation of isotopic glucosamine into sialic acid. Neuraminidase treatment failed to alter the rate of cell growth or the pattern of isotopic incorporation into various cell surface components. These results support the suggestion that return of sialic acid (repair) was effected by turnover which serves as a non-specific repair mechanism to replace damaged cell surface molecules (Warren and Glick '68; Warren, '69).", "PMID": 972162}
{"id": "pubmed23n0032_8853", "title": "Variation in melanosome numbers in cultured B-16 melanoma cells.", "content": "Melanosomes from B-16 mouse melanoma cells in culture were isolated by treatment of pigmented cells with 2% SDS, sonication, and heating at 100 degrees C. The total number of melanosomes in cultures of B-16 mouse melanoma cells increased exponentially during the rapid phase of sigmoid growth. The numbers of melanosomes per cell decreased during rapid phase of growth, and repigmentation was observed only when the cultures attained the stationary growth phase. BUdr at a minimum concentration of 0.5 mug/ml decreased both cell growth and numbers of melanosomes per cell, and completely inhibited repigmentation following a period of active growth. Cells cultured in 0.1 mug/ml BUdr grew at the same rate as untreated cells but contained fewer melanosomes/cell and lower total numbers of melanosomes during the late stages of the growth cycle.", "contents": "Variation in melanosome numbers in cultured B-16 melanoma cells. Melanosomes from B-16 mouse melanoma cells in culture were isolated by treatment of pigmented cells with 2% SDS, sonication, and heating at 100 degrees C. The total number of melanosomes in cultures of B-16 mouse melanoma cells increased exponentially during the rapid phase of sigmoid growth. The numbers of melanosomes per cell decreased during rapid phase of growth, and repigmentation was observed only when the cultures attained the stationary growth phase. BUdr at a minimum concentration of 0.5 mug/ml decreased both cell growth and numbers of melanosomes per cell, and completely inhibited repigmentation following a period of active growth. Cells cultured in 0.1 mug/ml BUdr grew at the same rate as untreated cells but contained fewer melanosomes/cell and lower total numbers of melanosomes during the late stages of the growth cycle.", "PMID": 972163}
{"id": "pubmed23n0032_8854", "title": "Purine uptake by azaguanine-resistant Chinese hamster cells.", "content": "In this study the resistance of a number of lines of Chinese hamster ovary cells to azaguanine is examined. Those which are drug resistant by virtue of a deficiency of hypoxanthine-guanine phosphoribosyltransferase (HPRT) fail to take up any exogenous hypoxanthine or azaguanine. A second class of drug resistant cells which grow in the reverse selective HAT medium and have levels of HPRT in the range of the wild type parent line take up these purines at lower rates than the nonresistant cells and incorporate smaller amounts of them into trichloracetic acidinsoluble constituents. The results suggest that their basis for resistance resides in lowered incorporation of azaguanine into DNA and RNA, possibly due to a mofified HPRT molecule which accepts hypoxanthine, but not azaguanine as a substrate.", "contents": "Purine uptake by azaguanine-resistant Chinese hamster cells. In this study the resistance of a number of lines of Chinese hamster ovary cells to azaguanine is examined. Those which are drug resistant by virtue of a deficiency of hypoxanthine-guanine phosphoribosyltransferase (HPRT) fail to take up any exogenous hypoxanthine or azaguanine. A second class of drug resistant cells which grow in the reverse selective HAT medium and have levels of HPRT in the range of the wild type parent line take up these purines at lower rates than the nonresistant cells and incorporate smaller amounts of them into trichloracetic acidinsoluble constituents. The results suggest that their basis for resistance resides in lowered incorporation of azaguanine into DNA and RNA, possibly due to a mofified HPRT molecule which accepts hypoxanthine, but not azaguanine as a substrate.", "PMID": 972164}
{"id": "pubmed23n0032_8855", "title": "Predilection of occlusive disease for the left iliac artery.", "content": "Eighty-six consecutive patients operated on for atherosclerotic occlusion of the aortoiliac segment were examined regarding the severity of the disease in the left or right iliac arteries. Sixty-nine percent of 78 patients were found to have predominantly more severe lesions on the left side. A study of the geometric anatomy of the aortoiliac region in 14 male and 12 female cadavers revealed that the right common iliac take-off angle was wider than the left and the radius of curvature of the right osculating circle at the aortoiliac bifurcation was smaller than the left, with the asymmetry of the region being much more marked in males than in females. The predilection of occlusive disease for the left iliac artery is explained on the basis of these differences in local geometric anatomy and their effect on the local hemodynamics.", "contents": "Predilection of occlusive disease for the left iliac artery. Eighty-six consecutive patients operated on for atherosclerotic occlusion of the aortoiliac segment were examined regarding the severity of the disease in the left or right iliac arteries. Sixty-nine percent of 78 patients were found to have predominantly more severe lesions on the left side. A study of the geometric anatomy of the aortoiliac region in 14 male and 12 female cadavers revealed that the right common iliac take-off angle was wider than the left and the radius of curvature of the right osculating circle at the aortoiliac bifurcation was smaller than the left, with the asymmetry of the region being much more marked in males than in females. The predilection of occlusive disease for the left iliac artery is explained on the basis of these differences in local geometric anatomy and their effect on the local hemodynamics.", "PMID": 972159}
{"id": "pubmed23n0032_8856", "title": "The effect of oxygen tension on the growth and metabolism of WI-38 cells.", "content": "The effect of oxygen tension on cellular growth and metabolism was studied in actively growing WI-38 cells [greater than 90% labeled nuclei (LN)] grown under atmospheres containing 5% CO2 and various combinations of O2 and N2. Cells grown under a partial pressure of oxygen (PO2) of 7.8 +/- 3.5 mm Hg had a significantly slower growth rate, lower saturation densities and higher rates of glucose consumption and lactate production than did cells grown under a PO2 of 44 +/- 7 mm Hg. There were no significant differences in saturation density or the rates of glucose consumption or lactate production between cells grown under PO2 26 +/- 4 mm Hg, 44 +/- 7 mm Hg, or 134 +/- 11 mm Hg. Population doubling time was slightly prolonged at a PO2 of 134 mm Hg compared to a PO2 of 44 mm Hg. Cells grown under a PO2 of 291 +/- 25 mm Hg showed only 20-30% of the growth rate and 10-20% of the saturation density of cells grown under a PO2 of 134 mm Hg. Despite this reduced growth, cells grown under a PO2 of 291 mm Hg consumed four to six times as much glucose and produced four to six times as much lactate per cell as cells grown at a PO2 of 134 mm Hg. Cells grown under a PO2 of 560 +/- 38 mm Hg attached but did not proliferate. This toxic effect of oxygen on cell proliferation was reversible and was not due to an effect of oxygen on the media.", "contents": "The effect of oxygen tension on the growth and metabolism of WI-38 cells. The effect of oxygen tension on cellular growth and metabolism was studied in actively growing WI-38 cells [greater than 90% labeled nuclei (LN)] grown under atmospheres containing 5% CO2 and various combinations of O2 and N2. Cells grown under a partial pressure of oxygen (PO2) of 7.8 +/- 3.5 mm Hg had a significantly slower growth rate, lower saturation densities and higher rates of glucose consumption and lactate production than did cells grown under a PO2 of 44 +/- 7 mm Hg. There were no significant differences in saturation density or the rates of glucose consumption or lactate production between cells grown under PO2 26 +/- 4 mm Hg, 44 +/- 7 mm Hg, or 134 +/- 11 mm Hg. Population doubling time was slightly prolonged at a PO2 of 134 mm Hg compared to a PO2 of 44 mm Hg. Cells grown under a PO2 of 291 +/- 25 mm Hg showed only 20-30% of the growth rate and 10-20% of the saturation density of cells grown under a PO2 of 134 mm Hg. Despite this reduced growth, cells grown under a PO2 of 291 mm Hg consumed four to six times as much glucose and produced four to six times as much lactate per cell as cells grown at a PO2 of 134 mm Hg. Cells grown under a PO2 of 560 +/- 38 mm Hg attached but did not proliferate. This toxic effect of oxygen on cell proliferation was reversible and was not due to an effect of oxygen on the media.", "PMID": 972165}
{"id": "pubmed23n0032_8857", "title": "Dystrophy of the media with aneurysmal tendency in the abdominal aorta-iliac segments (13 cases).", "content": "The authors report 13 cases of aneurysm of the abdominal aorta and common iliac arteries similar to those described by Leriche. The main morphological abnormality was major dystrophy involving the media, giving a special pathological appearance to these structures, long considered a mere morphological anomaly. In this part of the arterial system at least, they seem to evolve towards aneurysm and rupture (thin-walled aneurysm). This course requires echotomographic supervision of cases not submitted to immediate surgery, owing to the widespread nature of the process and the fact that some lesions resemble large arteries rather than aneurysms. The classic surgical technique of aortic and iliac aneurysms should often also include special technical devices. First, unilateral by-pass operation before the resection stage, and secondly aortic suture on a prosthesis to avoid tearing the arterial wall which is affected by media-spongiosis. The ten cases operated here all had a favourable course with a follow-up period of 1 to 9 years.", "contents": "Dystrophy of the media with aneurysmal tendency in the abdominal aorta-iliac segments (13 cases). The authors report 13 cases of aneurysm of the abdominal aorta and common iliac arteries similar to those described by Leriche. The main morphological abnormality was major dystrophy involving the media, giving a special pathological appearance to these structures, long considered a mere morphological anomaly. In this part of the arterial system at least, they seem to evolve towards aneurysm and rupture (thin-walled aneurysm). This course requires echotomographic supervision of cases not submitted to immediate surgery, owing to the widespread nature of the process and the fact that some lesions resemble large arteries rather than aneurysms. The classic surgical technique of aortic and iliac aneurysms should often also include special technical devices. First, unilateral by-pass operation before the resection stage, and secondly aortic suture on a prosthesis to avoid tearing the arterial wall which is affected by media-spongiosis. The ten cases operated here all had a favourable course with a follow-up period of 1 to 9 years.", "PMID": 972158}
{"id": "pubmed23n0032_8858", "title": "Stimulation of WI-38 cell cycle transit: effect of serum concentration and cell density.", "content": "Flow microfluorometry has been used to characterize the effects of serum concentration and cell density on the initiation of cell cycle transit of stationary phase (G0) human diploid fibroblasts (strain WI-38). The concentration of serum used to stimulate these cultures had no effect on the time cells began appearing in S (the DNA synthetic period), nor on the synchrony with which they moved around the cell cycle. However, as the serum concentration increased, the fraction of the stationary phase population released from G0 increased. Cell density modulated the ability of serum to stimulate cell cycle traverse. For example, at a cell density of 1.81 X 10(4) cells/cm2, 78% of the population was sensitive to serum stimulation; whereas, when the density was increased to 7.25 X 10(4) cells/cm2, only 27% of the population could be stimulated. This effect of cell density on the serum response is not simply the result of changing the ratio of serum concentration to cell density, but appears to reflect a true modulation of the population's sensitivity to serum stimulation. These results are consistent with the interpretation that the primary action of serum is to determine the transition of cells from a non-cycling G0 state to a cycling state and that cell density determines the proportion of the population capable of undergoing this transition.", "contents": "Stimulation of WI-38 cell cycle transit: effect of serum concentration and cell density. Flow microfluorometry has been used to characterize the effects of serum concentration and cell density on the initiation of cell cycle transit of stationary phase (G0) human diploid fibroblasts (strain WI-38). The concentration of serum used to stimulate these cultures had no effect on the time cells began appearing in S (the DNA synthetic period), nor on the synchrony with which they moved around the cell cycle. However, as the serum concentration increased, the fraction of the stationary phase population released from G0 increased. Cell density modulated the ability of serum to stimulate cell cycle traverse. For example, at a cell density of 1.81 X 10(4) cells/cm2, 78% of the population was sensitive to serum stimulation; whereas, when the density was increased to 7.25 X 10(4) cells/cm2, only 27% of the population could be stimulated. This effect of cell density on the serum response is not simply the result of changing the ratio of serum concentration to cell density, but appears to reflect a true modulation of the population's sensitivity to serum stimulation. These results are consistent with the interpretation that the primary action of serum is to determine the transition of cells from a non-cycling G0 state to a cycling state and that cell density determines the proportion of the population capable of undergoing this transition.", "PMID": 972166}
{"id": "pubmed23n0032_8859", "title": "Control of normal differentiation of myeloid leukemic cells. XI. Induction of a specific requirement for cell viability and growth during the differentiation of myeloid leukemic cells.", "content": "Normal hematopoetic cells require the presence of a protein (MGI) in the appropriate conditioned medium (CM) for cell viability and growth and for differentiation to mature macrophages and granulocytes. Clones of myeloid leukemic cells have been established in culture (D+ clones) which require CM with this protein for differentiation, but not for cell viability and growth. It has been shown that these leukemic cells can be induced by CM to again require, like normal cells, the presence of CM for cell viability and growth. Induction of this requirement, which will be referred to as RVG, occurred before the D+ cells differentiated to mature granulocytes. Clones of myeloid leukemic cells (D- clones) that could not be induced to differentiate to mature cells, did not show the induction of RVG. The steroid hormones prednisolone and dexamethasone can induce some, but not all the changes associated with differentiation of D+ cells, Incubation with these steroids did not result in the induction of a requirement for these steroids for cell growth and viability. Studies with CM from different sources have shown, that all batches that induced RVG also induced differentiation of D+ cells and that both activities were inhibited after treating the CM with trypsin. It is suggested that the same protein (MGI) may be involved in both activities. Incubation of D+ cells with CM resulted in an increase in agglutinability by concanavalin A and this increase was maintained even in the absence of CM. This suggests, that the induction of RVG in D+ myeloid leukemic cells is associated with a change in the cell surface membrane.", "contents": "Control of normal differentiation of myeloid leukemic cells. XI. Induction of a specific requirement for cell viability and growth during the differentiation of myeloid leukemic cells. Normal hematopoetic cells require the presence of a protein (MGI) in the appropriate conditioned medium (CM) for cell viability and growth and for differentiation to mature macrophages and granulocytes. Clones of myeloid leukemic cells have been established in culture (D+ clones) which require CM with this protein for differentiation, but not for cell viability and growth. It has been shown that these leukemic cells can be induced by CM to again require, like normal cells, the presence of CM for cell viability and growth. Induction of this requirement, which will be referred to as RVG, occurred before the D+ cells differentiated to mature granulocytes. Clones of myeloid leukemic cells (D- clones) that could not be induced to differentiate to mature cells, did not show the induction of RVG. The steroid hormones prednisolone and dexamethasone can induce some, but not all the changes associated with differentiation of D+ cells, Incubation with these steroids did not result in the induction of a requirement for these steroids for cell growth and viability. Studies with CM from different sources have shown, that all batches that induced RVG also induced differentiation of D+ cells and that both activities were inhibited after treating the CM with trypsin. It is suggested that the same protein (MGI) may be involved in both activities. Incubation of D+ cells with CM resulted in an increase in agglutinability by concanavalin A and this increase was maintained even in the absence of CM. This suggests, that the induction of RVG in D+ myeloid leukemic cells is associated with a change in the cell surface membrane.", "PMID": 972167}
{"id": "pubmed23n0032_8860", "title": "Enhanced synthesis and extracellular accumulation of hyaluronic acid during stimulation of quiescent human fibroblasts by mouse epidermal growth factor.", "content": "The effect of mouse epidermal growth factor (mEGF) on the synthesis of glycosaminoglycans and glycoproteins by human fibroblasts has been studied. The addition of physiological concentrations (10(-9)M) of mEGF to quiescent cultures preincubated in the absence of serum was found to elicit an increased incorporation of 3H-glucosamine into the glycosaminoglycans and glycoproteins of both the cellular and extracellular fractions. Although the growth response to the factor, as measured by DNA replication, was minimal under these conditions as compared with the effect of serum, the mEGF-induced incorporation of glucosamine into these cellular constituents and into the extracellular glycoproteins was comparable to that elicited by serum shift-up. Serum, however, caused a significantly larger incorporation of glucoasimine into extracellular, acid-soluble glycosaminoglycans, which were shown to contain hyaluronic acid as the major component. As previously demonstrated, the growth response to mEGF can be enhanced several fold by an mEGF-binding arginine esterase, which is normally associated with the factor in vivo, and by ascorbate. The esterase was found to increase markedly the mEGF-induced incorporation of glucosamine into extracellular hyaluronic acid, while the addition of ascorbic acid did not significantly alter glucosamine incorporation.", "contents": "Enhanced synthesis and extracellular accumulation of hyaluronic acid during stimulation of quiescent human fibroblasts by mouse epidermal growth factor. The effect of mouse epidermal growth factor (mEGF) on the synthesis of glycosaminoglycans and glycoproteins by human fibroblasts has been studied. The addition of physiological concentrations (10(-9)M) of mEGF to quiescent cultures preincubated in the absence of serum was found to elicit an increased incorporation of 3H-glucosamine into the glycosaminoglycans and glycoproteins of both the cellular and extracellular fractions. Although the growth response to the factor, as measured by DNA replication, was minimal under these conditions as compared with the effect of serum, the mEGF-induced incorporation of glucosamine into these cellular constituents and into the extracellular glycoproteins was comparable to that elicited by serum shift-up. Serum, however, caused a significantly larger incorporation of glucoasimine into extracellular, acid-soluble glycosaminoglycans, which were shown to contain hyaluronic acid as the major component. As previously demonstrated, the growth response to mEGF can be enhanced several fold by an mEGF-binding arginine esterase, which is normally associated with the factor in vivo, and by ascorbate. The esterase was found to increase markedly the mEGF-induced incorporation of glucosamine into extracellular hyaluronic acid, while the addition of ascorbic acid did not significantly alter glucosamine incorporation.", "PMID": 972168}
{"id": "pubmed23n0032_8861", "title": "Inhibition of sulfate transport in Ehrlich ascites tumor cells by 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid(SITS).", "content": "The effects of the nonpenetrating amino reactive reagnet 4-acetamido-4'-isothiocyano-stilbene-2-2'-dilsulfonic acid (SITS) on anion transport (sulfate, chloride, and inorganic phosphate) were investigated in Ehrlich ascites tumor cells. Short time exposure to SITS produces a reversible inhibition (92%) of sulfate transport. The kinetics of interaction suggest that reversibly bound SITS competitively inhibits sulfate transport, Ki = 3 X 10(-6)M. Incubation of tumor cells with SITS (1 X 10(-4)M) for longer periods of time results in a time dependent irreversible inhibition of sulfate transport which obeys first order kinetics. The rate coefficient for the inactivation process is 0.040 min-1. The kinetics of irreversible inhibition is best explained by the irreversible binding of SITS to the sulfate transport site, and therefore makes SITS a potentially useful probe for the quantiation of these sites in the tumor cell. The lack of effect of irreversibly bound SITS on either chloride or inorganic phosphate transport points to a specificity in the interaction of SITS with the tumor cell membrane, as well as indicating that an alternate pathway exists for the movement of these anions across the membrane.", "contents": "Inhibition of sulfate transport in Ehrlich ascites tumor cells by 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid(SITS). The effects of the nonpenetrating amino reactive reagnet 4-acetamido-4'-isothiocyano-stilbene-2-2'-dilsulfonic acid (SITS) on anion transport (sulfate, chloride, and inorganic phosphate) were investigated in Ehrlich ascites tumor cells. Short time exposure to SITS produces a reversible inhibition (92%) of sulfate transport. The kinetics of interaction suggest that reversibly bound SITS competitively inhibits sulfate transport, Ki = 3 X 10(-6)M. Incubation of tumor cells with SITS (1 X 10(-4)M) for longer periods of time results in a time dependent irreversible inhibition of sulfate transport which obeys first order kinetics. The rate coefficient for the inactivation process is 0.040 min-1. The kinetics of irreversible inhibition is best explained by the irreversible binding of SITS to the sulfate transport site, and therefore makes SITS a potentially useful probe for the quantiation of these sites in the tumor cell. The lack of effect of irreversibly bound SITS on either chloride or inorganic phosphate transport points to a specificity in the interaction of SITS with the tumor cell membrane, as well as indicating that an alternate pathway exists for the movement of these anions across the membrane.", "PMID": 972169}
{"id": "pubmed23n0032_8862", "title": "Regulation of lipid synthesis from acetate in diploid fibroblast cultures -- variation with passage level and stage of cell growth.", "content": "Regulation of lipid synthesis from acetate in human diploid fibroblast cultures has been studied at various passage levels and at different stages of cell growth. When cultures were transferred to lipid free medium, a stimulation of [14C]acetate incorporation into lipid occurred within three to six hours after removal of exogenous lipid. In early passage cultures, this stimulation was observed whether cells were transferred to protein-free medium or medium supplemented with delipidized serum protein. However, in late passage cultures the presence of delipidized serum protein was required for the stimulation of lipid synthesis. When logarithmically dividing and stationary phase cultures were compared, the cultures in log phase showed stimulation of acetate incorporation into lipid in the presence or absence of delipidized serum protein, whereas in the stationary cultures the delipidized serum protein was required. When cultures were partially synchronized by a thymidine block, stimulation of acetate incorporation into lipid in the blocked cells only occurred in the presence of delipidized serum protein; in released cells stimulation occurred in protein free medium. When inhibition of lipid synthesis from acetate was compared in young vs. old or dividing vs. stationary cultures, however, no differences were observed. The data indicate the response of diploid fibroblast cultres to change in exogenous lipid is dependent on passage level and state of growth.", "contents": "Regulation of lipid synthesis from acetate in diploid fibroblast cultures -- variation with passage level and stage of cell growth. Regulation of lipid synthesis from acetate in human diploid fibroblast cultures has been studied at various passage levels and at different stages of cell growth. When cultures were transferred to lipid free medium, a stimulation of [14C]acetate incorporation into lipid occurred within three to six hours after removal of exogenous lipid. In early passage cultures, this stimulation was observed whether cells were transferred to protein-free medium or medium supplemented with delipidized serum protein. However, in late passage cultures the presence of delipidized serum protein was required for the stimulation of lipid synthesis. When logarithmically dividing and stationary phase cultures were compared, the cultures in log phase showed stimulation of acetate incorporation into lipid in the presence or absence of delipidized serum protein, whereas in the stationary cultures the delipidized serum protein was required. When cultures were partially synchronized by a thymidine block, stimulation of acetate incorporation into lipid in the blocked cells only occurred in the presence of delipidized serum protein; in released cells stimulation occurred in protein free medium. When inhibition of lipid synthesis from acetate was compared in young vs. old or dividing vs. stationary cultures, however, no differences were observed. The data indicate the response of diploid fibroblast cultres to change in exogenous lipid is dependent on passage level and state of growth.", "PMID": 972170}
{"id": "pubmed23n0032_8863", "title": "Endosymbiosis in amoebae: recently established endosymbionts have become required cytoplasmic components.", "content": "A strain of large, free-living amoeba that became dependent on bacterial endosymbionts which had infected the amoebae initially as intracellular parasites, was studied by micrurgy and electron microscopy. The results show that the infected host cells require the presence of live endosymbionts for their survival.Thus, the nucleus of an infected amoeba can form a viable cell with the cytoplasm of a noninfected amoeba only when live endosymbionts are present. The endosymbiotic bacteria are not digested by the host amoebae and are not themselves used as nutritional supplement. While the host amoebae are dependent specifically on the endosymbionts, the latter can live inside amoebae of different strains, indicating that their dependence on the host cells is not yet strain specific.", "contents": "Endosymbiosis in amoebae: recently established endosymbionts have become required cytoplasmic components. A strain of large, free-living amoeba that became dependent on bacterial endosymbionts which had infected the amoebae initially as intracellular parasites, was studied by micrurgy and electron microscopy. The results show that the infected host cells require the presence of live endosymbionts for their survival.Thus, the nucleus of an infected amoeba can form a viable cell with the cytoplasm of a noninfected amoeba only when live endosymbionts are present. The endosymbiotic bacteria are not digested by the host amoebae and are not themselves used as nutritional supplement. While the host amoebae are dependent specifically on the endosymbionts, the latter can live inside amoebae of different strains, indicating that their dependence on the host cells is not yet strain specific.", "PMID": 972171}
{"id": "pubmed23n0032_8864", "title": "Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. VII. fibrillation in ventricle and auricle cells.", "content": "Rat myocardial cells in vitro were irradiated in individual mitochondria with an argon ion laser microbeam. The contractile respone termed fibrillation in single and multicellular groups of both ventricle and auricle cells were compared. Specific correlations were made between fibrillation duration, the number of cells in the group, and the number of times the cells had fibrillated. Correlations were also made for the number of laser shots needed to induce fibrillation and the number of cells in the group. Another set of correlations were made between the pre-irradiation beat frequency and the beat frequency following recovery. Several differences and similarities of the above parameters were detected between auricle and ventricle cells. A comparison of the morphology and ultrastructure of auricle and ventricle cells also revealed significant differences.", "contents": "Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. VII. fibrillation in ventricle and auricle cells. Rat myocardial cells in vitro were irradiated in individual mitochondria with an argon ion laser microbeam. The contractile respone termed fibrillation in single and multicellular groups of both ventricle and auricle cells were compared. Specific correlations were made between fibrillation duration, the number of cells in the group, and the number of times the cells had fibrillated. Correlations were also made for the number of laser shots needed to induce fibrillation and the number of cells in the group. Another set of correlations were made between the pre-irradiation beat frequency and the beat frequency following recovery. Several differences and similarities of the above parameters were detected between auricle and ventricle cells. A comparison of the morphology and ultrastructure of auricle and ventricle cells also revealed significant differences.", "PMID": 972172}
{"id": "pubmed23n0032_8865", "title": "Electron-microscope observations on cell nuclei in various tissues of a teleost fish: the nucleolus-associated monolayer of chromatin structural units.", "content": "Observations on stain uptake by thin sections through condensed interphase chromosomes in cells from epithelial and muscle tissue in kidney and intestine, and also in fibroblasts, show a distribution into DNA-rich and DNA-poor phases similar to that already described in cells from the connective tissue blood. In all the nuclei the nucleolus, when adjacent to the nuclear envelope, is separated from the inner membrane by a monolayer of chromatin structural units, similar to the monolayer enclosed on both sides by nuclear envelope, previously described in a wide variety of organisms. The data provide further support for the hypothesis that the condensed interphase chromosomes in eukaryotes are characterized by essentially similar structural units folded to form similar patterns. This hypothesis, regarding the higher order units, is consistent with data of others which show that histones and DNA fold to form similar repeating subunits in chromatin, irrespective of the base sequence in the DNA and the origin of the histones.", "contents": "Electron-microscope observations on cell nuclei in various tissues of a teleost fish: the nucleolus-associated monolayer of chromatin structural units. Observations on stain uptake by thin sections through condensed interphase chromosomes in cells from epithelial and muscle tissue in kidney and intestine, and also in fibroblasts, show a distribution into DNA-rich and DNA-poor phases similar to that already described in cells from the connective tissue blood. In all the nuclei the nucleolus, when adjacent to the nuclear envelope, is separated from the inner membrane by a monolayer of chromatin structural units, similar to the monolayer enclosed on both sides by nuclear envelope, previously described in a wide variety of organisms. The data provide further support for the hypothesis that the condensed interphase chromosomes in eukaryotes are characterized by essentially similar structural units folded to form similar patterns. This hypothesis, regarding the higher order units, is consistent with data of others which show that histones and DNA fold to form similar repeating subunits in chromatin, irrespective of the base sequence in the DNA and the origin of the histones.", "PMID": 972173}
{"id": "pubmed23n0032_8866", "title": "Ultrastructural site variations in mouse epidermal organization.", "content": "Mouse dorsal, ear, tail and foot epidermis are compared according to their tissue architecture and cell kinetics. Cell proliferation is expected in terms of the daily volume of keratin replaced. The stratum corneum may be organized into vertical columns of squames, which may have minimal overlap as in dorsum and ear, or maximal overlap as in tail. Individual areas are adapted to their function both in squame fine structure and rate of cell replacement. The surface keratin loss/replacement rate is at its highest in foot and tail, and lowest in ear and dorsum. Observations on hairless mouse dorsum are also included.", "contents": "Ultrastructural site variations in mouse epidermal organization. Mouse dorsal, ear, tail and foot epidermis are compared according to their tissue architecture and cell kinetics. Cell proliferation is expected in terms of the daily volume of keratin replaced. The stratum corneum may be organized into vertical columns of squames, which may have minimal overlap as in dorsum and ear, or maximal overlap as in tail. Individual areas are adapted to their function both in squame fine structure and rate of cell replacement. The surface keratin loss/replacement rate is at its highest in foot and tail, and lowest in ear and dorsum. Observations on hairless mouse dorsum are also included.", "PMID": 972174}
{"id": "pubmed23n0032_8867", "title": "The blepharoplast of Marsilea: its de novo formation and spindle association.", "content": "Blepharoplast formation and function has been investigated by light and electron microscopy in developing microspores of the water fern Marsilea vestita. When single-celled microspores are hydrated they undergo a series of 9 cell divisions producing 7 sterile cells plus 32 spermatids. The blepharoplast, a densely staining, spherical inclusion (0-5-1-0 mum in diameter) interpenetrated by numerous lightly stained channels, is a precursor organelle for the production of the 100-150 basal bodies of the motile sperm. During development it arises twice in the dividing spermatogenous cells; first in telophase of the second to last or 7th division, and again in telophase of the next to last or 8th division. The blepharoplast first forms on the distal side of the nucleus during telophase of the 7th division but soon degenerates as the cell enters prophase and metaphase of the 8th division. The blepharoplast first forms on the distal side of the nucleus during telophase of the 7th division but soon degenerates as the cell enters prophase and metaphase of the 8th division. During telophase of the 8th division the blepharoplast again arises in the cytoplasm near a small indentation of the nuclear envelope. The forming blepharoplast consists of a sphere of lightly stained flocculent material within which 2 plaques separated by 40-50 nm arise. Each plaque possesses 2 dense layers (20 nm) separated by a light layer (10 nm). During subsequent development the distal layer of each plaque becomes progressively more densely stained. Light-staining channels appear within the accumulating dense material a-d 2 hemispherical blepharoplasts emerge. The blepharoplasts become spherical through continued growth and finally separate from each other as they move to the spindle poles during prophase of the last or 9th division. The blepharoplast appears to act as a microtubule organizing centre during formation of the spindle apparatus in prophase of the 9th division. While it remains at the pole throughout mitosis it does not continue to serve as the focal point for the spindle tubules. During metaphase-anaphase of the 9th division the blepharoplast swells, and the channels at its surface begin transforming into procentrioles and finally basal bodies. The results support the contention that basal bodies in Marsilea arise de novo, since no preexisting template such as a centriolar pinwheel is observed and sine the intermediates which initially occur are structurally dissimilar from a procentriole.", "contents": "The blepharoplast of Marsilea: its de novo formation and spindle association. Blepharoplast formation and function has been investigated by light and electron microscopy in developing microspores of the water fern Marsilea vestita. When single-celled microspores are hydrated they undergo a series of 9 cell divisions producing 7 sterile cells plus 32 spermatids. The blepharoplast, a densely staining, spherical inclusion (0-5-1-0 mum in diameter) interpenetrated by numerous lightly stained channels, is a precursor organelle for the production of the 100-150 basal bodies of the motile sperm. During development it arises twice in the dividing spermatogenous cells; first in telophase of the second to last or 7th division, and again in telophase of the next to last or 8th division. The blepharoplast first forms on the distal side of the nucleus during telophase of the 7th division but soon degenerates as the cell enters prophase and metaphase of the 8th division. The blepharoplast first forms on the distal side of the nucleus during telophase of the 7th division but soon degenerates as the cell enters prophase and metaphase of the 8th division. During telophase of the 8th division the blepharoplast again arises in the cytoplasm near a small indentation of the nuclear envelope. The forming blepharoplast consists of a sphere of lightly stained flocculent material within which 2 plaques separated by 40-50 nm arise. Each plaque possesses 2 dense layers (20 nm) separated by a light layer (10 nm). During subsequent development the distal layer of each plaque becomes progressively more densely stained. Light-staining channels appear within the accumulating dense material a-d 2 hemispherical blepharoplasts emerge. The blepharoplasts become spherical through continued growth and finally separate from each other as they move to the spindle poles during prophase of the last or 9th division. The blepharoplast appears to act as a microtubule organizing centre during formation of the spindle apparatus in prophase of the 9th division. While it remains at the pole throughout mitosis it does not continue to serve as the focal point for the spindle tubules. During metaphase-anaphase of the 9th division the blepharoplast swells, and the channels at its surface begin transforming into procentrioles and finally basal bodies. The results support the contention that basal bodies in Marsilea arise de novo, since no preexisting template such as a centriolar pinwheel is observed and sine the intermediates which initially occur are structurally dissimilar from a procentriole.", "PMID": 972175}
{"id": "pubmed23n0032_8868", "title": "Ultrastructural and enzymic modulation of HeLa cells induced by sodium butyrate and the effects of cytochalasin B and colcemid.", "content": "Sodium butyrate causes HeLa cells to assume an elongated and jagged shape. Ultrastructurally this change is associated with the formation of bundles of microfilaments. Desmosomes were present between adjacent cells. No increase in microtubules was observed in the butyrate-treated cells. Butyrate induces an increase in the activity of 2 membrane-bound enzymes, alkaline phosphatase and 5'-nucleotidase; however, the activity of a third membrane enzyme, acetylcholine esterase, is reduced. The activities of the several other enzymes with different subcellular localizations are not significantly increased. Colcemid and cytochalasin B prevent or reverse the butyrate-mediated change in HeLa cell morphology and also partially inhibit the induction of alkaline phosphatase activity in these cells. The effect of cytochalasin B on alkaline phosphatase induction may be caused by a reduction in protein synthesis produced by this fungal metabolite.", "contents": "Ultrastructural and enzymic modulation of HeLa cells induced by sodium butyrate and the effects of cytochalasin B and colcemid. Sodium butyrate causes HeLa cells to assume an elongated and jagged shape. Ultrastructurally this change is associated with the formation of bundles of microfilaments. Desmosomes were present between adjacent cells. No increase in microtubules was observed in the butyrate-treated cells. Butyrate induces an increase in the activity of 2 membrane-bound enzymes, alkaline phosphatase and 5'-nucleotidase; however, the activity of a third membrane enzyme, acetylcholine esterase, is reduced. The activities of the several other enzymes with different subcellular localizations are not significantly increased. Colcemid and cytochalasin B prevent or reverse the butyrate-mediated change in HeLa cell morphology and also partially inhibit the induction of alkaline phosphatase activity in these cells. The effect of cytochalasin B on alkaline phosphatase induction may be caused by a reduction in protein synthesis produced by this fungal metabolite.", "PMID": 972176}
{"id": "pubmed23n0032_8869", "title": "Pollen-wall proteins: quantitative cytochemistry of the origins of intine and exine enzymes in Brassica oleracea.", "content": "Simultaneous coupling methods for detection of acid phosphatase and non-specific esterase produce a coloured reaction product that is quantitatively related to enzyme content in freeze-sectioned Brassica pollen and tapetal cells. The intine-located acid phosphatase has 2 periods of synthesis: the first in late vacuolate period, associated with the completion of deposition of the intine polysaccharides; the second during pollen maturation, apparently reflecting cytoplasmic synthesis, Esterase activity accumulates in the tapetal cells until dissolution at early maturation period, when there is a dramatic rise in pollen-wall esterase activity, reflecting the transfer from tapetum to exine cavities. These quantitative studies confirm the gametophytic and sporophytic origins of the intine and exine proteins.", "contents": "Pollen-wall proteins: quantitative cytochemistry of the origins of intine and exine enzymes in Brassica oleracea. Simultaneous coupling methods for detection of acid phosphatase and non-specific esterase produce a coloured reaction product that is quantitatively related to enzyme content in freeze-sectioned Brassica pollen and tapetal cells. The intine-located acid phosphatase has 2 periods of synthesis: the first in late vacuolate period, associated with the completion of deposition of the intine polysaccharides; the second during pollen maturation, apparently reflecting cytoplasmic synthesis, Esterase activity accumulates in the tapetal cells until dissolution at early maturation period, when there is a dramatic rise in pollen-wall esterase activity, reflecting the transfer from tapetum to exine cavities. These quantitative studies confirm the gametophytic and sporophytic origins of the intine and exine proteins.", "PMID": 972177}
{"id": "pubmed23n0032_8870", "title": "Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts.", "content": "The serological identification of Propionibacterium acnes, P. granulosum, and P. avidum, using trichloroacetic acid extracts, is described. With antisera prepared against reference strains, the method has been tested on 142 strains recently isolated from human skin. All except two of the strains could be identified serologically, and there was excellent agreement between the serological results and the fermentation pattern of the strains. Two serological types of P. acnes and two of P. avidum were identified, but only one of P. granulosum.", "contents": "Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts. The serological identification of Propionibacterium acnes, P. granulosum, and P. avidum, using trichloroacetic acid extracts, is described. With antisera prepared against reference strains, the method has been tested on 142 strains recently isolated from human skin. All except two of the strains could be identified serologically, and there was excellent agreement between the serological results and the fermentation pattern of the strains. Two serological types of P. acnes and two of P. avidum were identified, but only one of P. granulosum.", "PMID": 972178}
{"id": "pubmed23n0032_8871", "title": "Occurrence of Trichophyton megninii in Ontario. Identification with a simple cultural procedure.", "content": "Five strains of Trichophyton migninii were isolated in Toronto from four people. One patient probably was infected in Toronto since he had not visited the European endemic area. These were the first isolates made in Ontario; a requirement for L-histidine was determined. A cultural differentiation of T. migninii and T. rubrum is described. Attention is directed to the similarity of the metabolism for dextrose in Bromocresol purple milk dextrose agar by T. megninii and T. mentagrophytes which differs from T. rubrum.", "contents": "Occurrence of Trichophyton megninii in Ontario. Identification with a simple cultural procedure. Five strains of Trichophyton migninii were isolated in Toronto from four people. One patient probably was infected in Toronto since he had not visited the European endemic area. These were the first isolates made in Ontario; a requirement for L-histidine was determined. A cultural differentiation of T. migninii and T. rubrum is described. Attention is directed to the similarity of the metabolism for dextrose in Bromocresol purple milk dextrose agar by T. megninii and T. mentagrophytes which differs from T. rubrum.", "PMID": 972179}
{"id": "pubmed23n0032_8872", "title": "Plaque assay of rabies virus on porcine kidney cell monolayers.", "content": "The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests.", "contents": "Plaque assay of rabies virus on porcine kidney cell monolayers. The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests.", "PMID": 972180}
{"id": "pubmed23n0032_8873", "title": "Photostatic copying of immunoelectrophoretic patterns with the use of color print filters.", "content": "Immunoelectrophoretograms can be replicated inexpensively, rapidly, and to scale by the use of color print filters on a photostatic copy machine. By this method additional copies are readily available for patient charts, permanent record, and any other use.", "contents": "Photostatic copying of immunoelectrophoretic patterns with the use of color print filters. Immunoelectrophoretograms can be replicated inexpensively, rapidly, and to scale by the use of color print filters on a photostatic copy machine. By this method additional copies are readily available for patient charts, permanent record, and any other use.", "PMID": 972181}
{"id": "pubmed23n0032_8874", "title": "Comparison of urethral swabs, urine, and urinary sediment for the isolation of Chlamydia.", "content": "In 70 male patients with urethritis, Chlamydia was recovered from 18 urethral swabs (26%), 5 urinary sediments (7%), and 3 urine specimens (4%). All Chlamydia isolates were recovered from urethral swabs.", "contents": "Comparison of urethral swabs, urine, and urinary sediment for the isolation of Chlamydia. In 70 male patients with urethritis, Chlamydia was recovered from 18 urethral swabs (26%), 5 urinary sediments (7%), and 3 urine specimens (4%). All Chlamydia isolates were recovered from urethral swabs.", "PMID": 972182}
{"id": "pubmed23n0032_8875", "title": "Retention of preservative levels of formaldehyde in desiccated biological products.", "content": "Concentrations ranging from 8 to 100% of the preservative level of formalin (37% formaldehyde solution) were retained by desiccated biologics, with most products retaining about 50% regardless of the amount originally present.", "contents": "Retention of preservative levels of formaldehyde in desiccated biological products. Concentrations ranging from 8 to 100% of the preservative level of formalin (37% formaldehyde solution) were retained by desiccated biologics, with most products retaining about 50% regardless of the amount originally present.", "PMID": 972183}
{"id": "pubmed23n0032_8876", "title": "Retention of preservative levels of phenol in desiccated biological products.", "content": "Preservative levels of phenol retained by desiccated biological samples ranged from 3 to 73%, with highly proteinaceous samples retaining the most phenol.", "contents": "Retention of preservative levels of phenol in desiccated biological products. Preservative levels of phenol retained by desiccated biological samples ranged from 3 to 73%, with highly proteinaceous samples retaining the most phenol.", "PMID": 972184}
{"id": "pubmed23n0032_8877", "title": "Incidence of bacteremia in adults without infection.", "content": "To determine the incidence of bacteremia in healthy adults, blood cultures were obtained from 240 patients who had no demonstrable foci of infection. Five patients (2.1%) had positive blood cultures. Staphylococcus epidermidis was isolated from four patients and Alcaligenes faecalis from one. In each of these patients, the organism isolated probably represented contamination rather than bacteremia.", "contents": "Incidence of bacteremia in adults without infection. To determine the incidence of bacteremia in healthy adults, blood cultures were obtained from 240 patients who had no demonstrable foci of infection. Five patients (2.1%) had positive blood cultures. Staphylococcus epidermidis was isolated from four patients and Alcaligenes faecalis from one. In each of these patients, the organism isolated probably represented contamination rather than bacteremia.", "PMID": 972185}
{"id": "pubmed23n0032_8878", "title": "Clinical laboratory evaluation of the fifty-milliliter vacutainer blood culture tube.", "content": "The efficacy of the 50-ml vacutainer culture tube (Becton-Dickinson) for recovery of microorganisms from blood cultures was compared with laboratory-prepared blood culture media. The isolation of Bacteroides sp. was significantly higher in the 50-ml vacutainer culture tube whereas that of nonfermenting gram-negative rods and yeasts was higher in the in-house media. There was no significant difference in the recovery of all other organisms.", "contents": "Clinical laboratory evaluation of the fifty-milliliter vacutainer blood culture tube. The efficacy of the 50-ml vacutainer culture tube (Becton-Dickinson) for recovery of microorganisms from blood cultures was compared with laboratory-prepared blood culture media. The isolation of Bacteroides sp. was significantly higher in the 50-ml vacutainer culture tube whereas that of nonfermenting gram-negative rods and yeasts was higher in the in-house media. There was no significant difference in the recovery of all other organisms.", "PMID": 972186}
{"id": "pubmed23n0032_8879", "title": "Study on the usefulness of hypertonic culture media.", "content": "Specimens from 300 patients were studied using five to nine aerobic and anaerobic culture media, including five that were hypertonic, Groups studied included fever of unknown origin, suspected endocarditis, endocarditis during therapy, bacteremia during therapy, abscess and cellulitis, presumed infectious arthritis, renal transplantation during rejection, collagen disease, sarcoidosis, lymphoma, and colitis. Isolates in hypertonic media were reverted to parent form by agar passage. In only 5% of these selected cases were organisms found in hypertonic, but not conventional, media that appeared on the basis of repeated isolation and/or serological studies to come from the patient. Nine of the 16 appeared to be of major significance. The two groups in which use of highly enriched, hypertonic media seemed most helpful were suspected endocarditis and undefined meningitis with negative cultures using standard media. The most effective of the hypertonic media used was 0.3 M sucrose in brain heart infusion with 20% horse serum. In most instances, the organism grew only in the hypertonic sucrose, and in most cases it appeared in conventional rather than aberrant form. Hypertonic media, especially 0.3 M sucrose, are of substantial helpin a small number of carefully selected cases.", "contents": "Study on the usefulness of hypertonic culture media. Specimens from 300 patients were studied using five to nine aerobic and anaerobic culture media, including five that were hypertonic, Groups studied included fever of unknown origin, suspected endocarditis, endocarditis during therapy, bacteremia during therapy, abscess and cellulitis, presumed infectious arthritis, renal transplantation during rejection, collagen disease, sarcoidosis, lymphoma, and colitis. Isolates in hypertonic media were reverted to parent form by agar passage. In only 5% of these selected cases were organisms found in hypertonic, but not conventional, media that appeared on the basis of repeated isolation and/or serological studies to come from the patient. Nine of the 16 appeared to be of major significance. The two groups in which use of highly enriched, hypertonic media seemed most helpful were suspected endocarditis and undefined meningitis with negative cultures using standard media. The most effective of the hypertonic media used was 0.3 M sucrose in brain heart infusion with 20% horse serum. In most instances, the organism grew only in the hypertonic sucrose, and in most cases it appeared in conventional rather than aberrant form. Hypertonic media, especially 0.3 M sucrose, are of substantial helpin a small number of carefully selected cases.", "PMID": 972187}
{"id": "pubmed23n0032_8880", "title": "Septicemia due to Neisseria lactamica.", "content": "Neisseria lactamica was isolated from the blood of a pediatric patient who had signs of septicemia and otitis media. Organisms morphologically resembling Neisseria, as well as gram-positive cocci, were seen on a Gram stain of fluid from the middle ear. It is hypothesized that the N. lactamica septicemia was secondary to infection of the middle ear by this organism.", "contents": "Septicemia due to Neisseria lactamica. Neisseria lactamica was isolated from the blood of a pediatric patient who had signs of septicemia and otitis media. Organisms morphologically resembling Neisseria, as well as gram-positive cocci, were seen on a Gram stain of fluid from the middle ear. It is hypothesized that the N. lactamica septicemia was secondary to infection of the middle ear by this organism.", "PMID": 972188}
{"id": "pubmed23n0032_8881", "title": "Critical analysis of hypertonic medium and agitation in detection of bacteremia.", "content": "Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting and shaking on recovery were studied. The volume of the blood had a definite effect on the recovery rate. When inoculum size was held constant, recovery of aerobic and facultative organisms was maximal in vented and shaken bottles; the presence of sucrose had no demonstrable effect, recovery of anaerobes was maximal using an unvented bottle incubated under stationary conditions; a significantly greater recovery of facultatives and a marginally greater recovery of anaerobes was obtained with the hypertonic formulation. We conclude that a hypertonic formulation of MCB offers no advantage in the recovery of anaerobes but is of value in the recovery of facultatives and anaerobes. It is recommended that blood cultures be routinely inoculated into isotonic MCB and then vented and shaken for at least 4 hours, and hypertonic MCB incubated without venting or shaking.", "contents": "Critical analysis of hypertonic medium and agitation in detection of bacteremia. Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting and shaking on recovery were studied. The volume of the blood had a definite effect on the recovery rate. When inoculum size was held constant, recovery of aerobic and facultative organisms was maximal in vented and shaken bottles; the presence of sucrose had no demonstrable effect, recovery of anaerobes was maximal using an unvented bottle incubated under stationary conditions; a significantly greater recovery of facultatives and a marginally greater recovery of anaerobes was obtained with the hypertonic formulation. We conclude that a hypertonic formulation of MCB offers no advantage in the recovery of anaerobes but is of value in the recovery of facultatives and anaerobes. It is recommended that blood cultures be routinely inoculated into isotonic MCB and then vented and shaken for at least 4 hours, and hypertonic MCB incubated without venting or shaking.", "PMID": 972189}
{"id": "pubmed23n0032_8882", "title": "Method for phage typing group A type 49 streptococci.", "content": "A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type 49 streptococci isolated from 10 worldwide sources were examined by this method. Among these strains, five distinct subtypes (I through V) could be distinguished on the basis of their lytic patterns. Only a few of the type 49 strains could not be classified into one of these phage subtypes (6% using 100 X routine test dilution). Strains from a single source were generally homogeneous with respect to their phage subtype. The method proved useful in discriminating between type 49 strains isolated from different geographical sources and from the same place in different years. Studies in progress suggest that it may be useful for subtyping other strains of special epidemiological interest, such as strains of other serological types associated with nephritis.", "contents": "Method for phage typing group A type 49 streptococci. A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type 49 streptococci isolated from 10 worldwide sources were examined by this method. Among these strains, five distinct subtypes (I through V) could be distinguished on the basis of their lytic patterns. Only a few of the type 49 strains could not be classified into one of these phage subtypes (6% using 100 X routine test dilution). Strains from a single source were generally homogeneous with respect to their phage subtype. The method proved useful in discriminating between type 49 strains isolated from different geographical sources and from the same place in different years. Studies in progress suggest that it may be useful for subtyping other strains of special epidemiological interest, such as strains of other serological types associated with nephritis.", "PMID": 972190}
{"id": "pubmed23n0032_8883", "title": "Bacteriocin (klebocin) sensitivity typing of klebsiella.", "content": "High-titer preparations of Klebsiella bacteriocins (klebocins) were obtained by using mitomycin to induce standard strains of Klebsiella. Of 296 clinical isolates of Klebsiella, 67% could be typed on the basis of their sensitivity to klebocins. The method proposed in this paper affords a standard basis for the further development of klebocin typing as a suitable procedure for hospital laboratories concerned with epidemiological investigations of hospital-associated infections. Evidence is also provided to show that high-titered klebocin typing can be used in conjunction with biochemical typing to provide a sensitive epidemiological marker for Klebsiella.", "contents": "Bacteriocin (klebocin) sensitivity typing of klebsiella. High-titer preparations of Klebsiella bacteriocins (klebocins) were obtained by using mitomycin to induce standard strains of Klebsiella. Of 296 clinical isolates of Klebsiella, 67% could be typed on the basis of their sensitivity to klebocins. The method proposed in this paper affords a standard basis for the further development of klebocin typing as a suitable procedure for hospital laboratories concerned with epidemiological investigations of hospital-associated infections. Evidence is also provided to show that high-titered klebocin typing can be used in conjunction with biochemical typing to provide a sensitive epidemiological marker for Klebsiella.", "PMID": 972191}
{"id": "pubmed23n0032_8884", "title": "Primary isolation of Mycobacterium avium complex-serotype 6 on blood agar.", "content": "Mycobacterium avium complex-serotype 6 was isolated in pure culture on blood agar plates from inocula taken from the heart blood, lungs, liver, kidneys, and spleen of a naturally infected captive female opossum (Didelphis marsupialis virginiana). Repeat cultures from stored tissues and transfer of colonies from original blood agar plates revealed that the mycobacterium grew on tryptose, brilliant green, eosin-methylene blue. Sabouraud glucose, and mycobiotic agar plates and in Fletcher leptospira medium. The cultural, biochemical, and serological characteristics of the test isolate were compared with other mycobacteria. This is the first report to describe the primary isolation of a serotype from the M. avium complex from an animal species on blood agar or in Fletcher broth. In addition, this is the second documented report describing the isolation and identification of a mycobacterial species from the American opossum.", "contents": "Primary isolation of Mycobacterium avium complex-serotype 6 on blood agar. Mycobacterium avium complex-serotype 6 was isolated in pure culture on blood agar plates from inocula taken from the heart blood, lungs, liver, kidneys, and spleen of a naturally infected captive female opossum (Didelphis marsupialis virginiana). Repeat cultures from stored tissues and transfer of colonies from original blood agar plates revealed that the mycobacterium grew on tryptose, brilliant green, eosin-methylene blue. Sabouraud glucose, and mycobiotic agar plates and in Fletcher leptospira medium. The cultural, biochemical, and serological characteristics of the test isolate were compared with other mycobacteria. This is the first report to describe the primary isolation of a serotype from the M. avium complex from an animal species on blood agar or in Fletcher broth. In addition, this is the second documented report describing the isolation and identification of a mycobacterial species from the American opossum.", "PMID": 972192}
{"id": "pubmed23n0032_8885", "title": "Caprylate-thallous agar medium for selectively isolating Serratia and its utility in the clinical laboratory.", "content": "A defined agar medium (hereinafter designated caprylate-thallous [CT5 agar) containing 0.01% yeast extract, 0.1% caprylic (n-octanoic) acid, and 0.025% thallous sulfate is highly selective for all Serratia species and effectively discriminates against most non-Serratia strains likely to be in the same habitats. The selectivity of CT agar is demonstrated by the very high efficiency of colony formation (mean, 80.7% of that on a nonselective complex medium) on CT agar by known Serratia strains and the very low efficiency of colony formation (close to zero) on CT agar by bacterial strains known not to be Serratia. The utility of this medium in actual clinical laboratory practice is demonstrated by the more rapid and higher recovery of Serratia on this selective medium as compared to conventional procedures of in-tandem runs of 513 consecutive urine, feces, and sputum specimens. Pigmented and nonpigmented Serratia strains deliberately added to fecal specimens can be selectively and quantitatively recovered on CT agar. CT agar compares favorably with, or in some cases is an improvement over, other selective media which have been recommended for isolating Serratia. This selective CT agar medium could be quite useful in ecological surveys, especially those related to hospital-acquired infections.", "contents": "Caprylate-thallous agar medium for selectively isolating Serratia and its utility in the clinical laboratory. A defined agar medium (hereinafter designated caprylate-thallous [CT5 agar) containing 0.01% yeast extract, 0.1% caprylic (n-octanoic) acid, and 0.025% thallous sulfate is highly selective for all Serratia species and effectively discriminates against most non-Serratia strains likely to be in the same habitats. The selectivity of CT agar is demonstrated by the very high efficiency of colony formation (mean, 80.7% of that on a nonselective complex medium) on CT agar by known Serratia strains and the very low efficiency of colony formation (close to zero) on CT agar by bacterial strains known not to be Serratia. The utility of this medium in actual clinical laboratory practice is demonstrated by the more rapid and higher recovery of Serratia on this selective medium as compared to conventional procedures of in-tandem runs of 513 consecutive urine, feces, and sputum specimens. Pigmented and nonpigmented Serratia strains deliberately added to fecal specimens can be selectively and quantitatively recovered on CT agar. CT agar compares favorably with, or in some cases is an improvement over, other selective media which have been recommended for isolating Serratia. This selective CT agar medium could be quite useful in ecological surveys, especially those related to hospital-acquired infections.", "PMID": 972193}
{"id": "pubmed23n0032_8886", "title": "Recent experience with the complement fixation test in the laboratory diagnosis of rickettsial diseases in the United States.", "content": "Sera from patients suspected of having rickettsial infections were tested in the complement fixation test with antigens prepared from the rickettsiae of Rocky Mountain spotted fever (SF), rickettsial pox (RP), murine typhus, epidemic typhus, and from Rickettsia canada (RC). Eight units of antigen were used in all cases and two units in man. Only those patients with antibody titers of 1:16 or higher were included in the study. Largely on the basis of comparative titers, the patients were divided into two groups: 102 with SF and 35 with infections by one of the members of the typhus group. The antibody titers were higher with SF antigen than RP antigen in 72% of the SF patients, and in only two SF patients was the RP titer higher, and then by only one tube (twofold dilution). There seemed little advantage in including the RP antigen in the battery of rickettsial antigens. Cross-reaction with at least one of the typhus antigens was observed in the sera from 64% of the SF patients. It was extensive enough to be confusing (within one tube) in 17% with eight units of antigen, but the differentiation was more distinct with two units of antigen. The cross-reaction with typhus antigens was as frequent in children with SF as it was in adults; thus, it is unlikely that these cross-reactions resulted from previous typhus vaccination. The serological differentiation between murine typhus and epidemic typhus was frequently difficult, but the epidemiological background was distinct. Five patients had higher titers to RC antigen, and four of these may possibly have had RC infections.", "contents": "Recent experience with the complement fixation test in the laboratory diagnosis of rickettsial diseases in the United States. Sera from patients suspected of having rickettsial infections were tested in the complement fixation test with antigens prepared from the rickettsiae of Rocky Mountain spotted fever (SF), rickettsial pox (RP), murine typhus, epidemic typhus, and from Rickettsia canada (RC). Eight units of antigen were used in all cases and two units in man. Only those patients with antibody titers of 1:16 or higher were included in the study. Largely on the basis of comparative titers, the patients were divided into two groups: 102 with SF and 35 with infections by one of the members of the typhus group. The antibody titers were higher with SF antigen than RP antigen in 72% of the SF patients, and in only two SF patients was the RP titer higher, and then by only one tube (twofold dilution). There seemed little advantage in including the RP antigen in the battery of rickettsial antigens. Cross-reaction with at least one of the typhus antigens was observed in the sera from 64% of the SF patients. It was extensive enough to be confusing (within one tube) in 17% with eight units of antigen, but the differentiation was more distinct with two units of antigen. The cross-reaction with typhus antigens was as frequent in children with SF as it was in adults; thus, it is unlikely that these cross-reactions resulted from previous typhus vaccination. The serological differentiation between murine typhus and epidemic typhus was frequently difficult, but the epidemiological background was distinct. Five patients had higher titers to RC antigen, and four of these may possibly have had RC infections.", "PMID": 972194}
{"id": "pubmed23n0032_8887", "title": "Rapid method for the detection and identification of mycolic acids in aerobic actinomycetes and related bacteria.", "content": "A rapid method for the identification of lipids characteristic of the genera Corynebacterium, Mycobacterium, Nocardia, and the \"rhodochrous group\" has been developed. Modifications of previously described methods make this procedure suitable for use in the clinical laboratory. Thin-layer chromatography is used to demonstrate the presence of the lipid characteristic of Nocardia spp. (type A) in some corynebacteria, nocardias, and members of the \"rhodochrous group.\" Precipitation in ether and ethanol is used to demonstrate the presence of mycobacterial mycolic acids. Since this procedure can be carried out in less than 2 days and the lipids are extracted from the same batch of cells grown for diaminopimelic acid and whole-cell sugar analyses, it can readily be added to the battery of tests performed in reference laboratories that deal with aerobic actinomycetes and related bacteria.", "contents": "Rapid method for the detection and identification of mycolic acids in aerobic actinomycetes and related bacteria. A rapid method for the identification of lipids characteristic of the genera Corynebacterium, Mycobacterium, Nocardia, and the \"rhodochrous group\" has been developed. Modifications of previously described methods make this procedure suitable for use in the clinical laboratory. Thin-layer chromatography is used to demonstrate the presence of the lipid characteristic of Nocardia spp. (type A) in some corynebacteria, nocardias, and members of the \"rhodochrous group.\" Precipitation in ether and ethanol is used to demonstrate the presence of mycobacterial mycolic acids. Since this procedure can be carried out in less than 2 days and the lipids are extracted from the same batch of cells grown for diaminopimelic acid and whole-cell sugar analyses, it can readily be added to the battery of tests performed in reference laboratories that deal with aerobic actinomycetes and related bacteria.", "PMID": 972195}
{"id": "pubmed23n0032_8888", "title": "Use of Mueller-Hinton agar as amylase testing medium.", "content": "Readily available Mueller-Hinton agar was used to detect starch hydrolysis by Streptococcus bovis.", "contents": "Use of Mueller-Hinton agar as amylase testing medium. Readily available Mueller-Hinton agar was used to detect starch hydrolysis by Streptococcus bovis.", "PMID": 972196}
{"id": "pubmed23n0032_8889", "title": "Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve. I. Cell proliferation.", "content": "Electron microscopy and 3H-thymidine autoradiographic techniques were used to study the fine structure of proliferating cells in developing rat optic nerve. Before the closure of the optic canal almost all of the cells incorporating radioactive thymidine are ventricular cells, but after closure (16 days of gestation) the vast majority are differentiating astroblasts or oligodendroblasts. Labeled astroblasts show a range in their degree of differentiation; some cells lack 90 A cytoplasmic filaments while others have glial filaments and abundant cytoplasmic organelles. In contrast to astroblasts, all of the labeled oligodendroblasts are in the early stages of differentiation. The proliferation of oligodendroblasts starts at five days postnatal, approximately a day or two before the onset of myelination.During myelinogenesis a few of the labeled oligodendroblasts show presumptive connections to myelin sheaths. Microglial cells do not appear to play a major role in gliogenesis since they form less than 2% of all the labeled cells. The results of this study indicate that astroblasts and oligodendroblasts, rather than undifferentiated glioblasts, are the major source of macroglia. The finding that proliferating glia are in the processof differentiation agrees with recent studies which show that differentiated cells can divide.", "contents": "Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve. I. Cell proliferation. Electron microscopy and 3H-thymidine autoradiographic techniques were used to study the fine structure of proliferating cells in developing rat optic nerve. Before the closure of the optic canal almost all of the cells incorporating radioactive thymidine are ventricular cells, but after closure (16 days of gestation) the vast majority are differentiating astroblasts or oligodendroblasts. Labeled astroblasts show a range in their degree of differentiation; some cells lack 90 A cytoplasmic filaments while others have glial filaments and abundant cytoplasmic organelles. In contrast to astroblasts, all of the labeled oligodendroblasts are in the early stages of differentiation. The proliferation of oligodendroblasts starts at five days postnatal, approximately a day or two before the onset of myelination.During myelinogenesis a few of the labeled oligodendroblasts show presumptive connections to myelin sheaths. Microglial cells do not appear to play a major role in gliogenesis since they form less than 2% of all the labeled cells. The results of this study indicate that astroblasts and oligodendroblasts, rather than undifferentiated glioblasts, are the major source of macroglia. The finding that proliferating glia are in the processof differentiation agrees with recent studies which show that differentiated cells can divide.", "PMID": 972201}
{"id": "pubmed23n0032_8890", "title": "Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve. II. Time of origin.", "content": "The time of origin for astrocytes and oligodendrocytes in rat optic nerve was studied by 3H-thymidine autoradiographic techniques similar to those used in dating the time of origin for neurons. This study shows that astrocytes are formed throughout late embryonic and all of postnatal development, while oligodendrocytes are generated only during the postnatal period. A few astroglia undergo their final cell division as early as 15.5 days of gestation, but most astrocytes are not generated until the first week of postnatal development. Although the final cell division for more than half of the astrocytes takes place before the end of the first postnatal week, fully mature, fibrous astrocytes are not observed in electron micrographs until after 14 days of age. This time lag implies that the differentiation of these early generated cells takes place gradually over a 2-to 3-week interval. Oligodendroglia begin their final division a day or two before the onset of myelination (6-7 days postnatal), but the vast majority are produced during the period of myelinogenesis. After almost all of the axons have been myelinated, oligodendrocytes are still being generated in small numbers. These late forming cells are generally less differentiated in appearance than those formed earlier; this suggests that the degree of differentiated of oligodendrocytes may be dependent upon the number of axons available for myelination. As with astrocytes, oligodendrocytes show a lag of about two weeks from the time of final cell division until they transform into morphologically differentiated cells. In transverse sections of the optic nerve heavily labeled neuroglia are randomly distributed, indicating there are no temporal-radial gradients for the individual cell types. This observation taken together with the other information obtained from the present and the previous study (Stoff et al., '76) strongly suggest that the factors controlling gliogenesis are different from those governing neuronogenesis.", "contents": "Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve. II. Time of origin. The time of origin for astrocytes and oligodendrocytes in rat optic nerve was studied by 3H-thymidine autoradiographic techniques similar to those used in dating the time of origin for neurons. This study shows that astrocytes are formed throughout late embryonic and all of postnatal development, while oligodendrocytes are generated only during the postnatal period. A few astroglia undergo their final cell division as early as 15.5 days of gestation, but most astrocytes are not generated until the first week of postnatal development. Although the final cell division for more than half of the astrocytes takes place before the end of the first postnatal week, fully mature, fibrous astrocytes are not observed in electron micrographs until after 14 days of age. This time lag implies that the differentiation of these early generated cells takes place gradually over a 2-to 3-week interval. Oligodendroglia begin their final division a day or two before the onset of myelination (6-7 days postnatal), but the vast majority are produced during the period of myelinogenesis. After almost all of the axons have been myelinated, oligodendrocytes are still being generated in small numbers. These late forming cells are generally less differentiated in appearance than those formed earlier; this suggests that the degree of differentiated of oligodendrocytes may be dependent upon the number of axons available for myelination. As with astrocytes, oligodendrocytes show a lag of about two weeks from the time of final cell division until they transform into morphologically differentiated cells. In transverse sections of the optic nerve heavily labeled neuroglia are randomly distributed, indicating there are no temporal-radial gradients for the individual cell types. This observation taken together with the other information obtained from the present and the previous study (Stoff et al., '76) strongly suggest that the factors controlling gliogenesis are different from those governing neuronogenesis.", "PMID": 972202}
{"id": "pubmed23n0032_8891", "title": "The influence of phenobarbital on biotransformation of 25-hydroxycholecalciferol.", "content": "Altered vitamin D metabolism has been implicated as a cause of anticonvulsant-induced osteomalacia. Previous studies have demonstrated accelerated biotransformation of vitamin D3 to 25-hydroxycholecalciferol (25-OHD3). However, it is not known whether the 25-OHD3 is metabolized to 1,25-dihydroxycholecalciferol (1,25-(OH)2D3), the tissue-active metabolite of vitamin D3. This study using rats was undertaken to study the influence of phenobarbital on the biotransformation of 25-OHD3 to 1,25-(OH)2D3. The disappearance rate of 25-OHD3 was virtually the same in the pheno-barbital-treated group (re = 0.0615 pmole/min) and the control group (re - 0.0549 pmole/min). Similarly, the appearance rate of 1,25-(OH)2D3 was virtually the same in the treated group (ra = 0.0133 pmole/min) and the control group (ra = 0.0134 pmole/min). These data suggest that phenobarbital does not affect the biotransformation of 25-OHD3 to 1,25-(OH)2D3. The implication of this observation is that altered vitamin D metabolism does not account for phenobarbital-induced osteomalacia.", "contents": "The influence of phenobarbital on biotransformation of 25-hydroxycholecalciferol. Altered vitamin D metabolism has been implicated as a cause of anticonvulsant-induced osteomalacia. Previous studies have demonstrated accelerated biotransformation of vitamin D3 to 25-hydroxycholecalciferol (25-OHD3). However, it is not known whether the 25-OHD3 is metabolized to 1,25-dihydroxycholecalciferol (1,25-(OH)2D3), the tissue-active metabolite of vitamin D3. This study using rats was undertaken to study the influence of phenobarbital on the biotransformation of 25-OHD3 to 1,25-(OH)2D3. The disappearance rate of 25-OHD3 was virtually the same in the pheno-barbital-treated group (re = 0.0615 pmole/min) and the control group (re - 0.0549 pmole/min). Similarly, the appearance rate of 1,25-(OH)2D3 was virtually the same in the treated group (ra = 0.0133 pmole/min) and the control group (ra = 0.0134 pmole/min). These data suggest that phenobarbital does not affect the biotransformation of 25-OHD3 to 1,25-(OH)2D3. The implication of this observation is that altered vitamin D metabolism does not account for phenobarbital-induced osteomalacia.", "PMID": 972197}
{"id": "pubmed23n0032_8892", "title": "The pharmacologic effect of aerosolized terbutaline sulfate in exercise-induced bronchospasm.", "content": "Bronchospasm can be induced in asthmatics when exercised according to a multistage branching treadmill protocol that allows them to achieve 80 per cent of maximal age-predicted heart rate. This degree of exercise is usually achievable and allows inducible bronchospasm to occur. This present study was undertaken to investigate the effect of terbutaline sulfate aerosol in exercise-induced broncho-spasm. Asthmatics were exercised to 80 per cent of their maximal heart rate, and FEV1.0 and MMEFR were assessed while standing using a Jones Pulmonar II waterless spirometer at 5, 15, and 30 minutes after exercise. After a standard rest period defined by a return to baseline of FEV1.0, MMEFR, and heart rate for 30 minutes, the subjects were administered either 0.50 mg aerosolized terbutaline sulfate or placebo and then exercised again. The pulmonary function parameters were again recorded after this exercise. Preterbutaline and postplacebo exercise resulted in a significant reduction in FEV1.0 and MMEFR, while after treatment with terbutaline not only did bronchospasm not occur but bronchodilation occurred (P less than 0.01). Inhaled terbutaline appeared to normalize the exercise tolerance of the asthmatics and restore physiologic pulmonary airway conductance according to the parameters of FEV1.0 and MMEFR.", "contents": "The pharmacologic effect of aerosolized terbutaline sulfate in exercise-induced bronchospasm. Bronchospasm can be induced in asthmatics when exercised according to a multistage branching treadmill protocol that allows them to achieve 80 per cent of maximal age-predicted heart rate. This degree of exercise is usually achievable and allows inducible bronchospasm to occur. This present study was undertaken to investigate the effect of terbutaline sulfate aerosol in exercise-induced broncho-spasm. Asthmatics were exercised to 80 per cent of their maximal heart rate, and FEV1.0 and MMEFR were assessed while standing using a Jones Pulmonar II waterless spirometer at 5, 15, and 30 minutes after exercise. After a standard rest period defined by a return to baseline of FEV1.0, MMEFR, and heart rate for 30 minutes, the subjects were administered either 0.50 mg aerosolized terbutaline sulfate or placebo and then exercised again. The pulmonary function parameters were again recorded after this exercise. Preterbutaline and postplacebo exercise resulted in a significant reduction in FEV1.0 and MMEFR, while after treatment with terbutaline not only did bronchospasm not occur but bronchodilation occurred (P less than 0.01). Inhaled terbutaline appeared to normalize the exercise tolerance of the asthmatics and restore physiologic pulmonary airway conductance according to the parameters of FEV1.0 and MMEFR.", "PMID": 972199}
{"id": "pubmed23n0032_8893", "title": "The effect of cycloserine on pyridoxine-dependent metabolism in tuberculosis.", "content": "Measurements were made of urinary tryptophan metabolites of 13 tuberculosis patients in order to reveal characteristics of pyridoxine-dependent metabolism before and during cycloserine treatment. The abnormally high level of xanthurenic acid excretion in untreated patients suggests a decreased availability of pyridoxal phosphate related to the disease process. Although plasma cycloserine levels were kept high once therapy began, xanthurenic acid excretion before and after tryptophan load became progressively more normal as symptoms diminished. This observation suggests that the convulsions which may sometimes accompany cycloserine administration are not due to a direct pyridoxine antagonism by the drug. Throughout the study, no significant changes in 5-hydroxyindoleacetic acid excretion were observed. Presumably, the metabolic pathway of serotonin is unaffected by tryptophan loading, cycloserine administration, or the apparent pyridoxine depletion associated with tuberculosis.", "contents": "The effect of cycloserine on pyridoxine-dependent metabolism in tuberculosis. Measurements were made of urinary tryptophan metabolites of 13 tuberculosis patients in order to reveal characteristics of pyridoxine-dependent metabolism before and during cycloserine treatment. The abnormally high level of xanthurenic acid excretion in untreated patients suggests a decreased availability of pyridoxal phosphate related to the disease process. Although plasma cycloserine levels were kept high once therapy began, xanthurenic acid excretion before and after tryptophan load became progressively more normal as symptoms diminished. This observation suggests that the convulsions which may sometimes accompany cycloserine administration are not due to a direct pyridoxine antagonism by the drug. Throughout the study, no significant changes in 5-hydroxyindoleacetic acid excretion were observed. Presumably, the metabolic pathway of serotonin is unaffected by tryptophan loading, cycloserine administration, or the apparent pyridoxine depletion associated with tuberculosis.", "PMID": 972198}
{"id": "pubmed23n0032_8894", "title": "Recovery from deafferentation by cricket interneurons after reinnervation by their peripheral field.", "content": "The physiological and morphological recovery of cricket (Acheta domesticus) abdominal giant interneurons following varying periods of deafferentation and subsequent regeneration was examined. The principal afferent input to two identified interneurons was removed by surgically ablating an abdominal sensory appendage, the cercus. Deafferentation restricts the growth of the dendrites of the medial giant interneuron. Reinnervation by the peripheral sensory field leads to recovery of the dendrite length which is dependent on the time allowed for recovery. The response properties of the reinnervated neurons never completely recovers irregardless of how short the period of deafferentation. Reinnervated neurons respond more weakly to standard tones than do control neurons. This is due in part to faciliation of an inhibitory synaptic input which is activated by the control cercus. The results suggest that the balance between the excitatory and the inhibitory synaptic inputs to these interneurons is irrevocably altered by brief periods of deafferentation early in life.", "contents": "Recovery from deafferentation by cricket interneurons after reinnervation by their peripheral field. The physiological and morphological recovery of cricket (Acheta domesticus) abdominal giant interneurons following varying periods of deafferentation and subsequent regeneration was examined. The principal afferent input to two identified interneurons was removed by surgically ablating an abdominal sensory appendage, the cercus. Deafferentation restricts the growth of the dendrites of the medial giant interneuron. Reinnervation by the peripheral sensory field leads to recovery of the dendrite length which is dependent on the time allowed for recovery. The response properties of the reinnervated neurons never completely recovers irregardless of how short the period of deafferentation. Reinnervated neurons respond more weakly to standard tones than do control neurons. This is due in part to faciliation of an inhibitory synaptic input which is activated by the control cercus. The results suggest that the balance between the excitatory and the inhibitory synaptic inputs to these interneurons is irrevocably altered by brief periods of deafferentation early in life.", "PMID": 972203}
{"id": "pubmed23n0032_8895", "title": "Cells of origin of entorhinal cortical afferents to the hippocampus and fascia dentata of the rat.", "content": "The pathway from the entorhinal cortical region to the hippocampal formation has previously been shown to be comprised of two sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. The goal of the present investigation was to determine if these two pathways might originate from different cell populations within the entorhinal area. The cells of origin of these entorhinal pathways were identified by retrograde labeling with horseradish peroxidase (HRP). Injections which labeled the entorhinal terminal fields in both the fascia dentata and regio superior resulted in the retrograde labeling of two populations of cells in the entorhinal area. Ipsilateral to the injection, HRP reaction product was found in the cells of layer II (predominantly stellate cells) and the cells of layer III (predominantly pyramidal cells). Contralateral to the injections, however, the reaction product was found almost exclusively in the cells of layer III. With selective injections of the entorhinal terminal field in regio superior, only the cells of layer III were labeled, but these were labeled bilaterally. Selective injection of the entorhinal terminal field in the fascia dentata, however, resulted in the labeling of cells of layer II, but not of layer III, and these cells of layer II were labeled almost exclusively ipsilaterally. A very small number of labeled cells in layer II were, however, found contralateral to the injection as well. No labeled cells were found either in the presubiculum or parasubiculum following injections of the hippocampal formation. These cell populations were found capable of retrograde transport of HRP, however, since cells in both presubiculum and parasubiculum were labeled following HRP injections into the contralateral entorhinal area. These results suggest that the projections to the fascia dentata originate from the cells of layer II, while the projections to regio superior originate from the cells of layer III of the entorhinal region proper. The very slight crossed projection from the entorhinal area to the contralateral area dentata probably originates from the small population of cells in layer II which are labeled following HRP injections in the contralateral area dentata.", "contents": "Cells of origin of entorhinal cortical afferents to the hippocampus and fascia dentata of the rat. The pathway from the entorhinal cortical region to the hippocampal formation has previously been shown to be comprised of two sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. The goal of the present investigation was to determine if these two pathways might originate from different cell populations within the entorhinal area. The cells of origin of these entorhinal pathways were identified by retrograde labeling with horseradish peroxidase (HRP). Injections which labeled the entorhinal terminal fields in both the fascia dentata and regio superior resulted in the retrograde labeling of two populations of cells in the entorhinal area. Ipsilateral to the injection, HRP reaction product was found in the cells of layer II (predominantly stellate cells) and the cells of layer III (predominantly pyramidal cells). Contralateral to the injections, however, the reaction product was found almost exclusively in the cells of layer III. With selective injections of the entorhinal terminal field in regio superior, only the cells of layer III were labeled, but these were labeled bilaterally. Selective injection of the entorhinal terminal field in the fascia dentata, however, resulted in the labeling of cells of layer II, but not of layer III, and these cells of layer II were labeled almost exclusively ipsilaterally. A very small number of labeled cells in layer II were, however, found contralateral to the injection as well. No labeled cells were found either in the presubiculum or parasubiculum following injections of the hippocampal formation. These cell populations were found capable of retrograde transport of HRP, however, since cells in both presubiculum and parasubiculum were labeled following HRP injections into the contralateral entorhinal area. These results suggest that the projections to the fascia dentata originate from the cells of layer II, while the projections to regio superior originate from the cells of layer III of the entorhinal region proper. The very slight crossed projection from the entorhinal area to the contralateral area dentata probably originates from the small population of cells in layer II which are labeled following HRP injections in the contralateral area dentata.", "PMID": 972204}
{"id": "pubmed23n0032_8896", "title": "Method factors associated with assessment of child behavior: fact or artifact?", "content": "A sample of 64 phobic children between the ages of 6 and 16, with their general population matched controls, were rated by parents on the Louisville Behavior Check List and Louisville Fear survey and by School Behavior check list. The analysis of ratings by factor analysis revealed three instrument factors while a multiple discriminant analysis elicited a multidimensional phobic trait that crossed observers, situations, and instruments. The results are discussed in terms of the implications for multivariant analysis of behavior change as well as for classification of psychopathological disorders of childhood. The thesis is advanced that instrument factors elicited by factor analysis in psychotherapy change studies are statistical artifacts and that a multiple discriminant analysis is a more appropriate technique for the study of change as well as for classification.", "contents": "Method factors associated with assessment of child behavior: fact or artifact? A sample of 64 phobic children between the ages of 6 and 16, with their general population matched controls, were rated by parents on the Louisville Behavior Check List and Louisville Fear survey and by School Behavior check list. The analysis of ratings by factor analysis revealed three instrument factors while a multiple discriminant analysis elicited a multidimensional phobic trait that crossed observers, situations, and instruments. The results are discussed in terms of the implications for multivariant analysis of behavior change as well as for classification of psychopathological disorders of childhood. The thesis is advanced that instrument factors elicited by factor analysis in psychotherapy change studies are statistical artifacts and that a multiple discriminant analysis is a more appropriate technique for the study of change as well as for classification.", "PMID": 972205}
{"id": "pubmed23n0032_8897", "title": "The effects of biasing information on behavioral observations and rating scales.", "content": "The effects of biasing information on behavioral observations and rating scales were studied. Forty-one undergraduate students trained in making reliable behavioral observations were given differential expectations concerning the activity level of a target child. They then viewed videotape recordings of that child and tallied frequency counts of six behavioral categories simultaneously. In addition, subjects completed postexperimental rating scales composed of specific, identifiable behaviors in regard to the target child. Results indicated that, for the most part, neither the behavioral observations nor the rating scales were significantly affected by the biasing information. It is suggested that rating scales constructed of items as discrete and readily identifiable as those of behavioral observation measures may prove resistant to biasing effects.", "contents": "The effects of biasing information on behavioral observations and rating scales. The effects of biasing information on behavioral observations and rating scales were studied. Forty-one undergraduate students trained in making reliable behavioral observations were given differential expectations concerning the activity level of a target child. They then viewed videotape recordings of that child and tallied frequency counts of six behavioral categories simultaneously. In addition, subjects completed postexperimental rating scales composed of specific, identifiable behaviors in regard to the target child. Results indicated that, for the most part, neither the behavioral observations nor the rating scales were significantly affected by the biasing information. It is suggested that rating scales constructed of items as discrete and readily identifiable as those of behavioral observation measures may prove resistant to biasing effects.", "PMID": 972206}
{"id": "pubmed23n0032_8898", "title": "The cheating and intention of a partner as determinants of evaluative decisions among juvenile offenders.", "content": "Male juvenile offenders were asked to evaluate the work of an experimental partner (confederate) who completed a work task four times. The work partner cheated on two trials and did not cheat on two trials while working for a charity (one cheat and one noncheat trial) or for selfish gain (one cheat and one noncheat trial). It was found that subjects rated the work done on cheating trials lower than work done on noncheating trials. In addition, subjects shared less reward money on cheating trials relative to noncheating trials. However, the intentions of the conferate, defined as the reward recipient, failed to affect subjects' judgments. These results were described as supporting previous studies which have indicated that delinquent fail to account of others' intentions when making moral judgments.", "contents": "The cheating and intention of a partner as determinants of evaluative decisions among juvenile offenders. Male juvenile offenders were asked to evaluate the work of an experimental partner (confederate) who completed a work task four times. The work partner cheated on two trials and did not cheat on two trials while working for a charity (one cheat and one noncheat trial) or for selfish gain (one cheat and one noncheat trial). It was found that subjects rated the work done on cheating trials lower than work done on noncheating trials. In addition, subjects shared less reward money on cheating trials relative to noncheating trials. However, the intentions of the conferate, defined as the reward recipient, failed to affect subjects' judgments. These results were described as supporting previous studies which have indicated that delinquent fail to account of others' intentions when making moral judgments.", "PMID": 972207}
{"id": "pubmed23n0032_8899", "title": "An experiment in social engineering in serving the families of predelinquents.", "content": "One hundred and two predelinquent adolescents were randomly assigned to one of two conditions: an experimental condition in which behavioral contracting services were offered both at home and in school or a control condition in which clients were told that they could not be accomodated by project therapists and were informed about the possibility of their receiving treatment in other programs in the community. Most of those who were assigned to the control condition did not avail themselves of services in these other programs. Results indicate that, relative to the controls, the behaviorally treated youth scored small but statistically significant improvement relative to the controls on five measures: ratings of school behavior by the person who originally referred them for service, by their teachers, by their mothers, and by their fathers, and ratings of their parent-child interactions as measured by their mothers. Gains were also reported in mother's ratings of their children's behavior at home. The failure of significant gains to be made in two measures of school performance--grades and attendance--is explained by the failure of the treatment techniques to over-come an age-related deterioration in these areas found among most children in the participating schools. The failure of fathers to find significant improvement in their relationships with the referred adolescents is viewed as a consequence of unanticipated changes in the intrafamilial balance of power resulting from contracting services. Finally, a subsidiary analysis showed that the contracting service appeared to offer the greatest gain to the demographically defined sub-groups of youths who are among the population-at-risk in many juvenile courts.", "contents": "An experiment in social engineering in serving the families of predelinquents. One hundred and two predelinquent adolescents were randomly assigned to one of two conditions: an experimental condition in which behavioral contracting services were offered both at home and in school or a control condition in which clients were told that they could not be accomodated by project therapists and were informed about the possibility of their receiving treatment in other programs in the community. Most of those who were assigned to the control condition did not avail themselves of services in these other programs. Results indicate that, relative to the controls, the behaviorally treated youth scored small but statistically significant improvement relative to the controls on five measures: ratings of school behavior by the person who originally referred them for service, by their teachers, by their mothers, and by their fathers, and ratings of their parent-child interactions as measured by their mothers. Gains were also reported in mother's ratings of their children's behavior at home. The failure of significant gains to be made in two measures of school performance--grades and attendance--is explained by the failure of the treatment techniques to over-come an age-related deterioration in these areas found among most children in the participating schools. The failure of fathers to find significant improvement in their relationships with the referred adolescents is viewed as a consequence of unanticipated changes in the intrafamilial balance of power resulting from contracting services. Finally, a subsidiary analysis showed that the contracting service appeared to offer the greatest gain to the demographically defined sub-groups of youths who are among the population-at-risk in many juvenile courts.", "PMID": 972208}
{"id": "pubmed23n0032_8900", "title": "A motivational environment for behaviorally deviant junior high school students.", "content": "A contingency management program was established in a junior high school to better manage and educate students having histories of severe misconduct. School administration selected 46 eighth-graders having multiple suspensions for misbehavior. Students (N = 32) in two of the schools remained in traditional programs, serving as controls, whereas students (N = 14) in the third school participated in a token reinforcement program. Reinforcers provided in the afternoon were contingent upon achievement and discipline during morning academic periods. Home-based reinforcers were established to support school behavior. Compared with the control group, significant reductions in negative school behavior as well as greater increases in academic achievement were obtained for the treatment group, thus supporting the efficacy of contingency management for adolescents school misbehavior.", "contents": "A motivational environment for behaviorally deviant junior high school students. A contingency management program was established in a junior high school to better manage and educate students having histories of severe misconduct. School administration selected 46 eighth-graders having multiple suspensions for misbehavior. Students (N = 32) in two of the schools remained in traditional programs, serving as controls, whereas students (N = 14) in the third school participated in a token reinforcement program. Reinforcers provided in the afternoon were contingent upon achievement and discipline during morning academic periods. Home-based reinforcers were established to support school behavior. Compared with the control group, significant reductions in negative school behavior as well as greater increases in academic achievement were obtained for the treatment group, thus supporting the efficacy of contingency management for adolescents school misbehavior.", "PMID": 972209}
{"id": "pubmed23n0032_8901", "title": "Time-out with children. Effects of an explanation and brief parent training on child and parent behaviors.", "content": "The purpose of the present study was twofold: (a) to determine the effect of an explanation prior to or after time-out on child compliance and on child disruptive behavior during time-out and (b) to determine the effect of brief parent training in time-out on child and parent behaviors. Thirty-two mother-child pairs served as subjects and were assigned to one of the following four groups: control, time-out only, explanation prior to time-out, or explanation following time-out. Each mother-child pair was observed for one session under pretraining, training, and posttraining conditions. The results indicated that time-out significantly increased compliance but the addition of an explanation did not further alter the effectiveness of time-out. Training in the use of time-out decreased the incidence of maternal interruptions but did affect maternal responses that were not trained. Finally, following brief time-out training for noncompliance, the mothers used the procedure only 50% of the time following noncompliance.", "contents": "Time-out with children. Effects of an explanation and brief parent training on child and parent behaviors. The purpose of the present study was twofold: (a) to determine the effect of an explanation prior to or after time-out on child compliance and on child disruptive behavior during time-out and (b) to determine the effect of brief parent training in time-out on child and parent behaviors. Thirty-two mother-child pairs served as subjects and were assigned to one of the following four groups: control, time-out only, explanation prior to time-out, or explanation following time-out. Each mother-child pair was observed for one session under pretraining, training, and posttraining conditions. The results indicated that time-out significantly increased compliance but the addition of an explanation did not further alter the effectiveness of time-out. Training in the use of time-out decreased the incidence of maternal interruptions but did affect maternal responses that were not trained. Finally, following brief time-out training for noncompliance, the mothers used the procedure only 50% of the time following noncompliance.", "PMID": 972210}
{"id": "pubmed23n0032_8902", "title": "Overselectivity, developmental level, and overtraining in autistic and normal children.", "content": "Thirteen autistic children were compared to 13 normal children matched to them in mental age, on performance of a visual discrimination task. Form, color, and size were relevant and redundant cues. The groups did not differ significantly in mean trials to reach criterion or in breadth of learning, and both groups increased their breadth of learning after 50 trials of overtraining. Form was preferred to color and size by both autistic and normal children. Within each group, rank on mental age was highly correlated with rank in breadth of learning. Verbal and nonverbal autistic children did not differ in breadth of learning or in dimensional preference. Even nonverbal autistics equaled the performance of their normal controls. Our results suggest that overselective attention is better understood as part of a general developmental lag in cognition in autistic children than as a specific deficit underlying psychotic behavior.", "contents": "Overselectivity, developmental level, and overtraining in autistic and normal children. Thirteen autistic children were compared to 13 normal children matched to them in mental age, on performance of a visual discrimination task. Form, color, and size were relevant and redundant cues. The groups did not differ significantly in mean trials to reach criterion or in breadth of learning, and both groups increased their breadth of learning after 50 trials of overtraining. Form was preferred to color and size by both autistic and normal children. Within each group, rank on mental age was highly correlated with rank in breadth of learning. Verbal and nonverbal autistic children did not differ in breadth of learning or in dimensional preference. Even nonverbal autistics equaled the performance of their normal controls. Our results suggest that overselective attention is better understood as part of a general developmental lag in cognition in autistic children than as a specific deficit underlying psychotic behavior.", "PMID": 972211}
{"id": "pubmed23n0032_8903", "title": "Behavior problems of Anglo and Mexican-American children.", "content": "The present investigation compared Anglo (N = 1,999) and Mexican-American children (N = 192) on the four factors of the Behavior Problem Checklist (BPCL). Teachers provided general information and BPCL ratings. The Anglo children manifested more problems on three of the factors than did the Mexican-American children. Significant interactions of grade, sex, and social class with ethnic group were revealed for two of the factors.", "contents": "Behavior problems of Anglo and Mexican-American children. The present investigation compared Anglo (N = 1,999) and Mexican-American children (N = 192) on the four factors of the Behavior Problem Checklist (BPCL). Teachers provided general information and BPCL ratings. The Anglo children manifested more problems on three of the factors than did the Mexican-American children. Significant interactions of grade, sex, and social class with ethnic group were revealed for two of the factors.", "PMID": 972212}
{"id": "pubmed23n0032_8904", "title": "Incidence of exercise-induced asthma in children.", "content": "The incidence of exercise-induced asthma (EIA) was studied in 134 asthmatic and 102 nonasthmatic atopic children and compared to that in 56 nonatopic children. Pulmonary function tests measuring forced vital capacity (FVC) and 1-sec forced expiratory volume (FEV1) were performed on each child prior to and serially for 20 min following free running exercise. The incidences of EIA among the asthmatic and atopic nonasthmatic children were 63% and 41%, respectively. This phenomenon is widespread among allergic children and cannot be accurately predicted from the history. A simple and easily performed outpatient procedure is described for the diagnosis of EIA.", "contents": "Incidence of exercise-induced asthma in children. The incidence of exercise-induced asthma (EIA) was studied in 134 asthmatic and 102 nonasthmatic atopic children and compared to that in 56 nonatopic children. Pulmonary function tests measuring forced vital capacity (FVC) and 1-sec forced expiratory volume (FEV1) were performed on each child prior to and serially for 20 min following free running exercise. The incidences of EIA among the asthmatic and atopic nonasthmatic children were 63% and 41%, respectively. This phenomenon is widespread among allergic children and cannot be accurately predicted from the history. A simple and easily performed outpatient procedure is described for the diagnosis of EIA.", "PMID": 972213}
{"id": "pubmed23n0032_8905", "title": "Diagnosis of food allergy by the radioallergosorbent test.", "content": "The radioallergosorbent test (RAST) was positive in 52.5% of 200 sera representing 200 food hypersensitivities from 108 patients with a history of definite immediate-type reactions to foods. Corresponding prick test was performed for 170 of the sera. The latter test was positive in 70%, the RAST was positive in 52%, and iether prick test or RAST was positive in 74%. It is concluded that the RAST is positive less frequently than the prick test in the diagnosis of immediate-type food allergy in clinically sensitive patients, but that the performance of both tests increases slightly the possibility of confirming the diagnosis. However, the RAST is useful for further evaluating positive prick tests with foods that do not correlate with clinical hypersensitivity.", "contents": "Diagnosis of food allergy by the radioallergosorbent test. The radioallergosorbent test (RAST) was positive in 52.5% of 200 sera representing 200 food hypersensitivities from 108 patients with a history of definite immediate-type reactions to foods. Corresponding prick test was performed for 170 of the sera. The latter test was positive in 70%, the RAST was positive in 52%, and iether prick test or RAST was positive in 74%. It is concluded that the RAST is positive less frequently than the prick test in the diagnosis of immediate-type food allergy in clinically sensitive patients, but that the performance of both tests increases slightly the possibility of confirming the diagnosis. However, the RAST is useful for further evaluating positive prick tests with foods that do not correlate with clinical hypersensitivity.", "PMID": 972215}
{"id": "pubmed23n0032_8906", "title": "Reactivation of tuberculosis: a problem of aging.", "content": "Six examples demonstrate the reactivation of pulmonary tuberculosis in elderly patients. In 3 cases the tuberculosis became reactivated 25, 47 and 51 years respectively after the initial diagnosis. In aged patients with pulmonary symptoms and abnormal x-ray findings, the presence of reactivated tuberculosis should always be considered. Sometimes steroid or x-ray therapy for other disease may be the reactivating factor.", "contents": "Reactivation of tuberculosis: a problem of aging. Six examples demonstrate the reactivation of pulmonary tuberculosis in elderly patients. In 3 cases the tuberculosis became reactivated 25, 47 and 51 years respectively after the initial diagnosis. In aged patients with pulmonary symptoms and abnormal x-ray findings, the presence of reactivated tuberculosis should always be considered. Sometimes steroid or x-ray therapy for other disease may be the reactivating factor.", "PMID": 972234}
{"id": "pubmed23n0032_8907", "title": "Femorotibial bypass in the elderly for revascularization of the severely ischemic lower extremity.", "content": "In the elderly, revascularization of a severely ischemic lower extremity with occlusion of both femoral and popliteal arteries often can be achieved by surgical construction of a distal bypass to the tibial or peroneal arteries. An aggressive diagnostic and therapeutic approach is necessary, in an attempt to prevent recourse to primary amputation. Femoroperoneal or femorotibial bypass can be performed safely and is recommended in elderly patients with advanced ischemia of a lower extremity with absolute indications for surgical intervention, e.g., gangrene, gangrenous ulceration or rest pain. A significant number of limbs can be salvaged by this method. Although the mortality rate in the older age groups is predictably higher, the overall rate for this operation compares favorably with that for primary amputation.", "contents": "Femorotibial bypass in the elderly for revascularization of the severely ischemic lower extremity. In the elderly, revascularization of a severely ischemic lower extremity with occlusion of both femoral and popliteal arteries often can be achieved by surgical construction of a distal bypass to the tibial or peroneal arteries. An aggressive diagnostic and therapeutic approach is necessary, in an attempt to prevent recourse to primary amputation. Femoroperoneal or femorotibial bypass can be performed safely and is recommended in elderly patients with advanced ischemia of a lower extremity with absolute indications for surgical intervention, e.g., gangrene, gangrenous ulceration or rest pain. A significant number of limbs can be salvaged by this method. Although the mortality rate in the older age groups is predictably higher, the overall rate for this operation compares favorably with that for primary amputation.", "PMID": 972235}
{"id": "pubmed23n0032_8908", "title": "Physician's attitudes toward the ill aged and nursing homes.", "content": "A survey of physicians in private practice (exclusive of pediatricians) was conducted in a medium sized suburban city in the New York metropolitan area, to determine whether physicians' attitudes toward the ill aged and nursing homes were predictors of the quality of medical care available to area nursing home patients. Questionnaires were circulated to 302 practitioners. Of the 28 percent who responded, 32 percent were psychiatrists, 15 percent primary care physicians and 8 percent orthopedists. Physicians felt competent to manage the ill aged, although 50 percent had had no significant degree of exposure to geriatric medicine in their medical education, and 70 percent of the primary care group had had none. Primary care and older physicians were more likely to treat patients in nursing homes. Almost 40 percent viewed the nursing home as a place to die. Although 85 percent studied that physicians should be involved in the nursing home displacement process, only 21 percent believed that they continued to be in charge of their patients after placement. The findings demonstrate generalized medical disinterest in the care of ill aged patients in institutions. The persons responsible for awarding government grants and those involved in planning medical school curricula should pay more attention to the needs of the chronically ill aged.", "contents": "Physician's attitudes toward the ill aged and nursing homes. A survey of physicians in private practice (exclusive of pediatricians) was conducted in a medium sized suburban city in the New York metropolitan area, to determine whether physicians' attitudes toward the ill aged and nursing homes were predictors of the quality of medical care available to area nursing home patients. Questionnaires were circulated to 302 practitioners. Of the 28 percent who responded, 32 percent were psychiatrists, 15 percent primary care physicians and 8 percent orthopedists. Physicians felt competent to manage the ill aged, although 50 percent had had no significant degree of exposure to geriatric medicine in their medical education, and 70 percent of the primary care group had had none. Primary care and older physicians were more likely to treat patients in nursing homes. Almost 40 percent viewed the nursing home as a place to die. Although 85 percent studied that physicians should be involved in the nursing home displacement process, only 21 percent believed that they continued to be in charge of their patients after placement. The findings demonstrate generalized medical disinterest in the care of ill aged patients in institutions. The persons responsible for awarding government grants and those involved in planning medical school curricula should pay more attention to the needs of the chronically ill aged.", "PMID": 972236}
{"id": "pubmed23n0032_8909", "title": "Effect of size on the function of three day hospitals: the case for the small unit.", "content": "The work load of three day hospitals attached to active departments of Geriatric Medicine was studied over a one-year period. A significant difference in the functional efficiency of the three units was noted. The smaller 12-place and 15-place units provided effective short-term rehabilitation, whereas the 28-place unit did not. Size seemed to be the major factor in the success or failure of a given unit. This study throws serious doubts upon the wisdom of a policy of building large day hospitals with all their accompanying problems of staffing and transport. An effective unit for out-patient rehabilitation can be provided with 10 to 15 places per day if the staff members are chiefly remedial therapists. In the districts studied, a provision of 0.5 place per 1,000 of the population aged 65 or older would have been sufficient to meet the perceived need for rehabilitation. However, this would not have been sufficient to satisfy the requirement for out-patient investigation and medical treatment on a day basis.", "contents": "Effect of size on the function of three day hospitals: the case for the small unit. The work load of three day hospitals attached to active departments of Geriatric Medicine was studied over a one-year period. A significant difference in the functional efficiency of the three units was noted. The smaller 12-place and 15-place units provided effective short-term rehabilitation, whereas the 28-place unit did not. Size seemed to be the major factor in the success or failure of a given unit. This study throws serious doubts upon the wisdom of a policy of building large day hospitals with all their accompanying problems of staffing and transport. An effective unit for out-patient rehabilitation can be provided with 10 to 15 places per day if the staff members are chiefly remedial therapists. In the districts studied, a provision of 0.5 place per 1,000 of the population aged 65 or older would have been sufficient to meet the perceived need for rehabilitation. However, this would not have been sufficient to satisfy the requirement for out-patient investigation and medical treatment on a day basis.", "PMID": 972237}
{"id": "pubmed23n0032_8910", "title": "Use of the Holter electrocardiographic monitor in the diagnosis of transient ischemic attacks.", "content": "The diagnosis of transient ischemic attacks usually is based on an accurate history and physical examination (with the finding of carotid bruits), a complete neurologic examination, a brain scan, an encephalogram and an angiogram. Recently the Holter technique for continuous 24-hour electrocardiographic monitoring has been used as a diagnostic aid in these problem cases. Severe bradycardia, sick sinus syndrome, tachy-brady syndrome, recurrent tachycardia and heart block have been found to be the cause of the central-nervous-system symptoms in aged atherosclerotic patients. Correction with anti-arrhythmia drugs and pacemakers, with or without carotid endarterectomy, has been highly successful. Data are presented on 204 patients tested by Holter monitoring over a 2.5-year period in a small community hospital; 6 cases are illustrated in detail. The Holter monitor is a valuable tool in evaluating the patients with cerebrovascular insufficiency.", "contents": "Use of the Holter electrocardiographic monitor in the diagnosis of transient ischemic attacks. The diagnosis of transient ischemic attacks usually is based on an accurate history and physical examination (with the finding of carotid bruits), a complete neurologic examination, a brain scan, an encephalogram and an angiogram. Recently the Holter technique for continuous 24-hour electrocardiographic monitoring has been used as a diagnostic aid in these problem cases. Severe bradycardia, sick sinus syndrome, tachy-brady syndrome, recurrent tachycardia and heart block have been found to be the cause of the central-nervous-system symptoms in aged atherosclerotic patients. Correction with anti-arrhythmia drugs and pacemakers, with or without carotid endarterectomy, has been highly successful. Data are presented on 204 patients tested by Holter monitoring over a 2.5-year period in a small community hospital; 6 cases are illustrated in detail. The Holter monitor is a valuable tool in evaluating the patients with cerebrovascular insufficiency.", "PMID": 972238}
{"id": "pubmed23n0032_8911", "title": "Behavioral change in the chronicity pattern of dementia in the institutional geriatric resident.", "content": "To determine whether behavioral change as a result of environmental stimulation can be reported in individualized terms for the aged with organic brain syndrome or senile dementia, a program of multimodal group interactive therapy was started in an extended care facility. During a 1.5-year period, the study included 108 residents, whose average age was 84 years. The technique used was a combination of modified Reality Orientation, Remotivation, Activity and Milieu therapies. Thirteen groups residing in different wings of the facility were treated for 55 sessions by 11 activity specialists and 2 elderly volunteers under the supervision of the consulting clinical psychologist. Data were collected on emerging behavioral changes with respect to awareness, attention, verbalized ideation, voice quality, speech quantity, sociability, self-care, quality of affect, energy level, expressed somatic concern and physical movement. Of the initial 108 residents, 80 had chronic brain syndrome of varying severity or senile dementia. During treatment, 35 of these 80 residents (44 percent) were improved, 17 (20 percent) were unchanged, 14 (18 percent) became worse, and 14 (18 percent) remained unrated. The results warrant a redifinition of the psychotherapeutic approach. It should include factors other than the severity and chronicity of disease in determining the prognosis for behavioral change in geriatric institutional residents.", "contents": "Behavioral change in the chronicity pattern of dementia in the institutional geriatric resident. To determine whether behavioral change as a result of environmental stimulation can be reported in individualized terms for the aged with organic brain syndrome or senile dementia, a program of multimodal group interactive therapy was started in an extended care facility. During a 1.5-year period, the study included 108 residents, whose average age was 84 years. The technique used was a combination of modified Reality Orientation, Remotivation, Activity and Milieu therapies. Thirteen groups residing in different wings of the facility were treated for 55 sessions by 11 activity specialists and 2 elderly volunteers under the supervision of the consulting clinical psychologist. Data were collected on emerging behavioral changes with respect to awareness, attention, verbalized ideation, voice quality, speech quantity, sociability, self-care, quality of affect, energy level, expressed somatic concern and physical movement. Of the initial 108 residents, 80 had chronic brain syndrome of varying severity or senile dementia. During treatment, 35 of these 80 residents (44 percent) were improved, 17 (20 percent) were unchanged, 14 (18 percent) became worse, and 14 (18 percent) remained unrated. The results warrant a redifinition of the psychotherapeutic approach. It should include factors other than the severity and chronicity of disease in determining the prognosis for behavioral change in geriatric institutional residents.", "PMID": 972239}
{"id": "pubmed23n0032_8912", "title": "Inheritance of 6-phosphogluconate dehydrogenase variants in Culex pipiens quinquefasciatus Say.", "content": "The formal genetics of two variants of a 6-phosphogluconate dehydrogenase locus of the adult mosquito, Culex pipiens quinquefasciatus, was analyzed by starch gel electrophoresis. These two alleles, PgdF and PgdS are codominant, and autosomally inherited, and appear to be linked to the Pgm locus by 27 crossover units in linkage group III.", "contents": "Inheritance of 6-phosphogluconate dehydrogenase variants in Culex pipiens quinquefasciatus Say. The formal genetics of two variants of a 6-phosphogluconate dehydrogenase locus of the adult mosquito, Culex pipiens quinquefasciatus, was analyzed by starch gel electrophoresis. These two alleles, PgdF and PgdS are codominant, and autosomally inherited, and appear to be linked to the Pgm locus by 27 crossover units in linkage group III.", "PMID": 972249}
{"id": "pubmed23n0032_8913", "title": "Selection effects on a linked neutral locus.", "content": "Selection changes the frequency of alleles at a linked locus as well as at those under selection if the population is not in linkage equilibrium. The magnitude of this frequency change depends on the tightness of the linkage, the selection intensity, and the deviation from linkage equilibrium. Allowing a population to mate randomly without selection brings the population closer to linkage equilibrium. This decreases the effect of selection on allelic frequencies at a linked neutral locus. However, if linkage is very tight it can take many generations to make a large difference in the effect of the linked locus. The loss due to undesirable changes in allelic frequencies at linked loci when the population is not in linkage equilibrium must be weighed against the time and effort saved by beginning intense selection for the primary trait in an early generation. Effects of selection intensity, linkage intensity, and delayed selection on changes in allelic frequency at a neutral linked locus are demonstrated.", "contents": "Selection effects on a linked neutral locus. Selection changes the frequency of alleles at a linked locus as well as at those under selection if the population is not in linkage equilibrium. The magnitude of this frequency change depends on the tightness of the linkage, the selection intensity, and the deviation from linkage equilibrium. Allowing a population to mate randomly without selection brings the population closer to linkage equilibrium. This decreases the effect of selection on allelic frequencies at a linked neutral locus. However, if linkage is very tight it can take many generations to make a large difference in the effect of the linked locus. The loss due to undesirable changes in allelic frequencies at linked loci when the population is not in linkage equilibrium must be weighed against the time and effort saved by beginning intense selection for the primary trait in an early generation. Effects of selection intensity, linkage intensity, and delayed selection on changes in allelic frequency at a neutral linked locus are demonstrated.", "PMID": 972250}
{"id": "pubmed23n0032_8914", "title": "Inheritance of limber legs in Jersey cattle.", "content": "Pedigree information from 105 cases of limber leg Jersey calves indicate that this semilethal abnormality is inherited as a simple autosomal recessive. The increased incidence of this abnormality in recent years suggests a rise in the gene frequency, probably due to the popularity and widespread use of a carrier bull Favorite Commando, his daughter Marlu Milady, and many of their descendants. Steps to control this abnormality have been taken by the American Jersey Cattle Club.", "contents": "Inheritance of limber legs in Jersey cattle. Pedigree information from 105 cases of limber leg Jersey calves indicate that this semilethal abnormality is inherited as a simple autosomal recessive. The increased incidence of this abnormality in recent years suggests a rise in the gene frequency, probably due to the popularity and widespread use of a carrier bull Favorite Commando, his daughter Marlu Milady, and many of their descendants. Steps to control this abnormality have been taken by the American Jersey Cattle Club.", "PMID": 972251}
{"id": "pubmed23n0032_8915", "title": "A further study of the inheritance of racing performance in thoroughbred horses.", "content": "In an analysis of handicap ratings of 1158 3-year-old thoroughbred racehorses, estimates were obtained for the effects of sex differences, the correlation between mates, the effectiveness of selection in males and females, and the heritability of racing performance. The results agreed closely with those of a previous study, and indicated a heritability of about 0.35 to 0.40, some assortative mating, and highly effective selection for performance.", "contents": "A further study of the inheritance of racing performance in thoroughbred horses. In an analysis of handicap ratings of 1158 3-year-old thoroughbred racehorses, estimates were obtained for the effects of sex differences, the correlation between mates, the effectiveness of selection in males and females, and the heritability of racing performance. The results agreed closely with those of a previous study, and indicated a heritability of about 0.35 to 0.40, some assortative mating, and highly effective selection for performance.", "PMID": 972252}
{"id": "pubmed23n0032_8916", "title": "Immunoelectronmicroscopic localization of IgA in skin of patients with dermatitis herpetiformis.", "content": "Ultrastructural localization of in vivo-bound IgA in the skin of patients with dermatitis herpetiformis was determined by using a modified peroxidase-antiperoxidase multistep method. Three types of reaction product deposition are seen. The most common type of reaction product deposition, that which is identified by direct immunofluorescence as the speckled type of IgA deposit, shows up as clumps and yarnlike fibrils. The second type of IgA deposition, which is a linear band by direct immunofluorescence, appears to be associated with anchoring fibrils. The third of IgA deposition, which is also linear by immunofluorescence, is confined to the lamina lucida.", "contents": "Immunoelectronmicroscopic localization of IgA in skin of patients with dermatitis herpetiformis. Ultrastructural localization of in vivo-bound IgA in the skin of patients with dermatitis herpetiformis was determined by using a modified peroxidase-antiperoxidase multistep method. Three types of reaction product deposition are seen. The most common type of reaction product deposition, that which is identified by direct immunofluorescence as the speckled type of IgA deposit, shows up as clumps and yarnlike fibrils. The second type of IgA deposition, which is a linear band by direct immunofluorescence, appears to be associated with anchoring fibrils. The third of IgA deposition, which is also linear by immunofluorescence, is confined to the lamina lucida.", "PMID": 972257}
{"id": "pubmed23n0032_8917", "title": "Ultrastructural localization of immunoglobulins in skin of patients with dermatitis herpetiformis.", "content": "A multistep immunocytochemical method utilizing horseradish peroxidase as an immunologically bound marker was used to detect and localize IgA deposits in skin of patients with dermatitis herpetiformis at the ultrastructural level. IgA was found in the upper papillary dermis forming irregular aggregates in seemingly haphazard distribution. These aggregates were associated with microfibrillar bundles and with the microfibrillar component of the elastic tissue. IgA was also detected on anchoring fibrils, but showed no topical relationship to the basal lamina which was always spared. This finding indicates that basal lamina components do not serve as target sites for the immunologic reaction occurring in dermatitis herpetiformis. The selective affinity of IgA deposits to microbfibrillar bundles may be relevant to the hypothesis that the skin pathology in dermatitis herpetiformis is caused by circulating gluten-antigluten complexes, trapped in the skin by reticulin-bound antireticulin antibodies which cross-react with gluten.", "contents": "Ultrastructural localization of immunoglobulins in skin of patients with dermatitis herpetiformis. A multistep immunocytochemical method utilizing horseradish peroxidase as an immunologically bound marker was used to detect and localize IgA deposits in skin of patients with dermatitis herpetiformis at the ultrastructural level. IgA was found in the upper papillary dermis forming irregular aggregates in seemingly haphazard distribution. These aggregates were associated with microfibrillar bundles and with the microfibrillar component of the elastic tissue. IgA was also detected on anchoring fibrils, but showed no topical relationship to the basal lamina which was always spared. This finding indicates that basal lamina components do not serve as target sites for the immunologic reaction occurring in dermatitis herpetiformis. The selective affinity of IgA deposits to microbfibrillar bundles may be relevant to the hypothesis that the skin pathology in dermatitis herpetiformis is caused by circulating gluten-antigluten complexes, trapped in the skin by reticulin-bound antireticulin antibodies which cross-react with gluten.", "PMID": 972258}
{"id": "pubmed23n0032_8918", "title": "Black bands on a starch gel stained for serum esterases in red grouse.", "content": "From 36 crosses of red grouse (Lagopus l. scoticus), 136 offspring were coded for a new locus (Rg). The three possible phenotypes were probably determined by two codominant alleles at an autosomal locus.", "contents": "Black bands on a starch gel stained for serum esterases in red grouse. From 36 crosses of red grouse (Lagopus l. scoticus), 136 offspring were coded for a new locus (Rg). The three possible phenotypes were probably determined by two codominant alleles at an autosomal locus.", "PMID": 972254}
{"id": "pubmed23n0032_8919", "title": "Prolonged ultraviolet light-induced erythema and the cutaneous carcinoma phenotype.", "content": "A considerable amount of evidence exists in support of the role of ultraviolet radiation as a major etiologic factor in human skin cancer, both melanoma and carcinoma types. On the basis of epidemiologic studies a phenotype has been described which helps to identify the persons who are more susceptible to skin cancer. In an attempt to further define this population, patients with cutaneous carcinoma and a normal control group were exposed to artificial ultraviolet light (UVL) and the erythema and tanning responses of each group were measured over a 21-day period. UVL-induced erythema was prolonged in a significantly higher percentage of patients with skin cancer than in control patients, lasting two to three weeks after single exposures to 6 and 8 times the patient's minimal erythema dose. The presence of prolonged erythema correlated with this history of previous skin cancer but did not correlate with other established risk factors for cutaneous carcinoma, i.e., fair skin, light hair and light eyes, easy sunburning and poor tanning, and Celtic ancestry. Prolonged erythema following UVL radiation may therefore represent an additional risk factor and help to identify the skin cancer-susceptible population.", "contents": "Prolonged ultraviolet light-induced erythema and the cutaneous carcinoma phenotype. A considerable amount of evidence exists in support of the role of ultraviolet radiation as a major etiologic factor in human skin cancer, both melanoma and carcinoma types. On the basis of epidemiologic studies a phenotype has been described which helps to identify the persons who are more susceptible to skin cancer. In an attempt to further define this population, patients with cutaneous carcinoma and a normal control group were exposed to artificial ultraviolet light (UVL) and the erythema and tanning responses of each group were measured over a 21-day period. UVL-induced erythema was prolonged in a significantly higher percentage of patients with skin cancer than in control patients, lasting two to three weeks after single exposures to 6 and 8 times the patient's minimal erythema dose. The presence of prolonged erythema correlated with this history of previous skin cancer but did not correlate with other established risk factors for cutaneous carcinoma, i.e., fair skin, light hair and light eyes, easy sunburning and poor tanning, and Celtic ancestry. Prolonged erythema following UVL radiation may therefore represent an additional risk factor and help to identify the skin cancer-susceptible population.", "PMID": 972259}
{"id": "pubmed23n0032_8920", "title": "Chromosome complexity in the Indian long-tailed tree mouse.", "content": "The chromosomes of the Indian long-tailed tree mouse, Vandeleuria oleracea nilagirica, have been studied in both sexes and the diploid number established to be 2n = 28 with a simple XX/XY sex chromosome mechanism. The sex chromosome polymorphism encountered in different taxa is discussed in the light of the present findings.", "contents": "Chromosome complexity in the Indian long-tailed tree mouse. The chromosomes of the Indian long-tailed tree mouse, Vandeleuria oleracea nilagirica, have been studied in both sexes and the diploid number established to be 2n = 28 with a simple XX/XY sex chromosome mechanism. The sex chromosome polymorphism encountered in different taxa is discussed in the light of the present findings.", "PMID": 972253}
{"id": "pubmed23n0032_8921", "title": "Randomized trial of a hexachlorophene preparation and P-300 bacteriostatic soaps.", "content": "Twenty-six patients undergoing chemotherapy for acute leukemia on a protected environment-prophylactic antibiotic program were randomly assigned to bathe with either P-300 or a 3% hexachlorophene preparation. Both agents substantially reduced the microbial flora of the skin, but the latter preparation was slightly more effective. The bacteriostatic compounds in both preparations were absorbed through the skin but no toxic effects were observed.", "contents": "Randomized trial of a hexachlorophene preparation and P-300 bacteriostatic soaps. Twenty-six patients undergoing chemotherapy for acute leukemia on a protected environment-prophylactic antibiotic program were randomly assigned to bathe with either P-300 or a 3% hexachlorophene preparation. Both agents substantially reduced the microbial flora of the skin, but the latter preparation was slightly more effective. The bacteriostatic compounds in both preparations were absorbed through the skin but no toxic effects were observed.", "PMID": 972260}
{"id": "pubmed23n0032_8922", "title": "8-methoxypsoralen and UVA promote sister-chromatid exchanges.", "content": "8-Methoxypsoralen (8-MOP) and long-wave UV light (UVA 365 nm) are now being use to treat vitiligo and psoriasis. Cultured human lymphocytes exposed to these agents in vitro show an increased infrequency of sister chromatid exchanges, related to 8-MOP-DNA photoadducts. Although these data raise questions regarding the biologic consequences of this therapeutic regimen, it is unknown whether 8-MOP and UVA cause mutations or extracutaneous somatic cell recombinants in vivo. Exchanges may represent cellular repair of DNA damage.", "contents": "8-methoxypsoralen and UVA promote sister-chromatid exchanges. 8-Methoxypsoralen (8-MOP) and long-wave UV light (UVA 365 nm) are now being use to treat vitiligo and psoriasis. Cultured human lymphocytes exposed to these agents in vitro show an increased infrequency of sister chromatid exchanges, related to 8-MOP-DNA photoadducts. Although these data raise questions regarding the biologic consequences of this therapeutic regimen, it is unknown whether 8-MOP and UVA cause mutations or extracutaneous somatic cell recombinants in vivo. Exchanges may represent cellular repair of DNA damage.", "PMID": 972261}
{"id": "pubmed23n0032_8923", "title": "The variation of recurrence risks with penetrance for isolated cases of autosomal dominant conditions.", "content": "The relationship between the penetrance and the recurrence risk for sibs of isolated cases of autosomal dominant conditions with incomplete penetrance is presented.", "contents": "The variation of recurrence risks with penetrance for isolated cases of autosomal dominant conditions. The relationship between the penetrance and the recurrence risk for sibs of isolated cases of autosomal dominant conditions with incomplete penetrance is presented.", "PMID": 972255}
{"id": "pubmed23n0032_8924", "title": "Renal glycosuria due to gentamicin in rabbits.", "content": "Gentamicin sulfate was given to rabbits for four weeks in two dosage regimens, either 4 or 8 mg/kg subcutaneously twice a day. Two of the seven animals in each group regained nearly normal renal function after a transient rise in the level of serum creatinine, but the remainder developed severe renal failure leading to the death of all but one of these rabbits. None of the animals showed significant hyperglycemia, buy glycosuria appeared in all and was significantly correlated with the extent of renal damage. These findings demonstrate gentamicin-induced derangement of proximal tubular function and thus amplify the previously demonstrated histologic changes due to the drug. When the drug was administered twice daily, there was marked nephrotoxicity in rabbits given dosages of gentamicin only slightly higher than those employed clinically.", "contents": "Renal glycosuria due to gentamicin in rabbits. Gentamicin sulfate was given to rabbits for four weeks in two dosage regimens, either 4 or 8 mg/kg subcutaneously twice a day. Two of the seven animals in each group regained nearly normal renal function after a transient rise in the level of serum creatinine, but the remainder developed severe renal failure leading to the death of all but one of these rabbits. None of the animals showed significant hyperglycemia, buy glycosuria appeared in all and was significantly correlated with the extent of renal damage. These findings demonstrate gentamicin-induced derangement of proximal tubular function and thus amplify the previously demonstrated histologic changes due to the drug. When the drug was administered twice daily, there was marked nephrotoxicity in rabbits given dosages of gentamicin only slightly higher than those employed clinically.", "PMID": 972262}
{"id": "pubmed23n0032_8925", "title": "Hepatitis B surface antigen in environmental samples from hemodialysis units.", "content": "For assessment of environmental contamination with hepatitis B surface antigen (HBS Ag), the presence of blood and of HBS Ag was determined in samples from two completely separated hemodialysis units. Samples from mucosal and skin surfaces and samples of saliva from patients were also investigated. The surfaces of both units were divided into epidemiological categories to determine the distribution of environmental contamination with HBS Ag. HBS Ag was determined by radioimmunoassay. Oral samples from all patients seropositive for HBS Ag were psoitive for HBS Ag. The rectum and the skin were positive in three instances. The presence of blood did not coincide with HBS Ag. In the unit in which HBS Ag-positive patients were treated, 4.3% of the total samples were HBS Ag-positive. Of the samples that were free of blood, 1.5% were HBS Ag-positive, whereas 18.5% of the blood-contaminated samples contained the antigen. HBS Ag-positive samples were taken from surfaces in the immediate environment of HBS Ag-positive patients, as well as from surfaces with which patients had no direct physical contact. The data lead to the conclusion that spilled blood and hemodialysis equipment are not the only sources of contamination. This fact implies that HBS Ag-positive patient undergoing hemodialysis should be completely separated from HBS Ag-negative patients.", "contents": "Hepatitis B surface antigen in environmental samples from hemodialysis units. For assessment of environmental contamination with hepatitis B surface antigen (HBS Ag), the presence of blood and of HBS Ag was determined in samples from two completely separated hemodialysis units. Samples from mucosal and skin surfaces and samples of saliva from patients were also investigated. The surfaces of both units were divided into epidemiological categories to determine the distribution of environmental contamination with HBS Ag. HBS Ag was determined by radioimmunoassay. Oral samples from all patients seropositive for HBS Ag were psoitive for HBS Ag. The rectum and the skin were positive in three instances. The presence of blood did not coincide with HBS Ag. In the unit in which HBS Ag-positive patients were treated, 4.3% of the total samples were HBS Ag-positive. Of the samples that were free of blood, 1.5% were HBS Ag-positive, whereas 18.5% of the blood-contaminated samples contained the antigen. HBS Ag-positive samples were taken from surfaces in the immediate environment of HBS Ag-positive patients, as well as from surfaces with which patients had no direct physical contact. The data lead to the conclusion that spilled blood and hemodialysis equipment are not the only sources of contamination. This fact implies that HBS Ag-positive patient undergoing hemodialysis should be completely separated from HBS Ag-negative patients.", "PMID": 972263}
{"id": "pubmed23n0032_8926", "title": "Coagulopathy in experimental sepsis with Streptococcus pneumoniae in rabbits: effect of drug therapy and splenectomy.", "content": "The cause of death in bacteremia due to Streptococcus pneumoniae remains unclear. The role of intravascular coagulation and splenectomy was investigated in rabbits with lethal pneumococcal bacteremia. The staphylococcal clumping titer in serum, a measure of fibrin degradation products, increased early and persisted until death. This titer correlated with the level of bacteremia. The partial thromboplastin time and platelet-rich plasma clotting time also increased as the disease worsened. However, the prothrombin time remained normal. 125I-labeled fibrinogen was cleared normally from the plasma of infected rabbits, whether intact or splenectomized. Similarly, the concentration of fibrogen in plasma remained normal, even though the level of fibrin degradation products increased, and no difference in these parameters was noted between intact and splenectomized rabbits. Fibrin deposition could not be detected in any of the organs studied. Neither the level of fibrin degradation products nor survival was affected by treatment with hydrocortisone, hexadimethrine, cytochrome c, carboxypeptidase B, epsilon-aminocaproic acid, or heparin. These data suggest that intravascular coagulation occurs in this experimental infection prior to the onset of shock but probably plays only a minor role in lethality.", "contents": "Coagulopathy in experimental sepsis with Streptococcus pneumoniae in rabbits: effect of drug therapy and splenectomy. The cause of death in bacteremia due to Streptococcus pneumoniae remains unclear. The role of intravascular coagulation and splenectomy was investigated in rabbits with lethal pneumococcal bacteremia. The staphylococcal clumping titer in serum, a measure of fibrin degradation products, increased early and persisted until death. This titer correlated with the level of bacteremia. The partial thromboplastin time and platelet-rich plasma clotting time also increased as the disease worsened. However, the prothrombin time remained normal. 125I-labeled fibrinogen was cleared normally from the plasma of infected rabbits, whether intact or splenectomized. Similarly, the concentration of fibrogen in plasma remained normal, even though the level of fibrin degradation products increased, and no difference in these parameters was noted between intact and splenectomized rabbits. Fibrin deposition could not be detected in any of the organs studied. Neither the level of fibrin degradation products nor survival was affected by treatment with hydrocortisone, hexadimethrine, cytochrome c, carboxypeptidase B, epsilon-aminocaproic acid, or heparin. These data suggest that intravascular coagulation occurs in this experimental infection prior to the onset of shock but probably plays only a minor role in lethality.", "PMID": 972264}
{"id": "pubmed23n0032_8927", "title": "Significance of diuresis-provoked bacteriuria.", "content": "Of 52 clinically healthy, ambulant elderly subjects with urine that was sterile or contained less than 0.2 X 10(5) bacteria/ml before diureisi, 11 (21.2%) responded to oral water loading and intravenous treatment with furosemide with a bacterial excretion rate of greater than 2.4 X 10(5)/min. Subjects with bacteriuria had decreased blood pressure, rate of creatinine clearance, maximal level of urinary creatinine, and maximal urinary flow. Twenty-nine of the nonbacteriuric and eight of the subjects who developed bacteriuria after diuresis were retested one to two years later. No subject had received antibacterial agents in the interim. The infections of four of the original subjects who had developed bacteriuria had cleared up and showed functional improvement. The remaining four subjects who had developed bacteriuria during the first study and three subjects who had been nonbacteriuric during the first study had concentrations of bacteria ranging from 0.05 X 10(5) to 1.80 X 10(5)/ml before diuresis in the second study, and excreted increasing numbers of bacteria (from 0.12 X 10(5) to 9.0 X 10(5)/min) in response to the diuretic loading. Two of the subjects who were bacteriuric in both surveys were infected with different bacteria during the two studies. The data show the high frequency of occult bacteriuria in ostensibly healthy elderly patients that is revealed only by forced diuresis, the detectable functional deficits, the improvement after clearing of the infections, and the rapid turnover between infected and clear periods.", "contents": "Significance of diuresis-provoked bacteriuria. Of 52 clinically healthy, ambulant elderly subjects with urine that was sterile or contained less than 0.2 X 10(5) bacteria/ml before diureisi, 11 (21.2%) responded to oral water loading and intravenous treatment with furosemide with a bacterial excretion rate of greater than 2.4 X 10(5)/min. Subjects with bacteriuria had decreased blood pressure, rate of creatinine clearance, maximal level of urinary creatinine, and maximal urinary flow. Twenty-nine of the nonbacteriuric and eight of the subjects who developed bacteriuria after diuresis were retested one to two years later. No subject had received antibacterial agents in the interim. The infections of four of the original subjects who had developed bacteriuria had cleared up and showed functional improvement. The remaining four subjects who had developed bacteriuria during the first study and three subjects who had been nonbacteriuric during the first study had concentrations of bacteria ranging from 0.05 X 10(5) to 1.80 X 10(5)/ml before diuresis in the second study, and excreted increasing numbers of bacteria (from 0.12 X 10(5) to 9.0 X 10(5)/min) in response to the diuretic loading. Two of the subjects who were bacteriuric in both surveys were infected with different bacteria during the two studies. The data show the high frequency of occult bacteriuria in ostensibly healthy elderly patients that is revealed only by forced diuresis, the detectable functional deficits, the improvement after clearing of the infections, and the rapid turnover between infected and clear periods.", "PMID": 972265}
{"id": "pubmed23n0032_8928", "title": "Studies on opacity factor production by pharyngeal isolates of group A streptococci.", "content": "Production of opacity factor (OF) by 841 pharyngeal isolates of group A streptococci from schoolchildren in Casper, Wyoming was studied along with T- and M-typing of the streptococcal strains. The majority (89%) of M-typable strains were OF-negative in contrast to M-nontypable isolates, 45% of which were OF-positive. There was no apparent relation between the production of OF by the M-nontypable isolates and the type of infection (symptomatic vs. asymptomatic), degree of positivity of initial culture, antistreptolysin O response, and failure to eradicate the organisms by treatment with antibiotics. However, seasonal shifts in the prevalence of M-nontypable OF-positive strains provided evidence of change in streptococcal ecology, such a shift would not have been detected by T-typing alone. Thus, OF production is an additional epidemiologic marker that is helpful in differentiating M-nontypable group A strains that bear common T-antigens.", "contents": "Studies on opacity factor production by pharyngeal isolates of group A streptococci. Production of opacity factor (OF) by 841 pharyngeal isolates of group A streptococci from schoolchildren in Casper, Wyoming was studied along with T- and M-typing of the streptococcal strains. The majority (89%) of M-typable strains were OF-negative in contrast to M-nontypable isolates, 45% of which were OF-positive. There was no apparent relation between the production of OF by the M-nontypable isolates and the type of infection (symptomatic vs. asymptomatic), degree of positivity of initial culture, antistreptolysin O response, and failure to eradicate the organisms by treatment with antibiotics. However, seasonal shifts in the prevalence of M-nontypable OF-positive strains provided evidence of change in streptococcal ecology, such a shift would not have been detected by T-typing alone. Thus, OF production is an additional epidemiologic marker that is helpful in differentiating M-nontypable group A strains that bear common T-antigens.", "PMID": 972266}
{"id": "pubmed23n0032_8929", "title": "Intravenous injection of gentamicin and tobramycin without impairment of hearing.", "content": "The aminoglycoside antibiotics gentamicin and tobramycin were given to six healthy volunteers by intravenous injection. A dose of 80 mg was injected within 1 min. The curves relating serum concentration to time did not differ significantly for the two drugs. The maximal serum concentrations of drug exceeded 10 mug/ml on seven of 12 occasions. Despite these high concentrations, loss of hearing was not significant at any frequency (1,000-8,000 Hz) up to one month after injection. It is suggested that the aminoglycoside antibiotics penetrate into the inner ear lymph very slowly and that the concentration at that site is relatively unaffected by transient high concentrations of antibiotic in the blood plasma.", "contents": "Intravenous injection of gentamicin and tobramycin without impairment of hearing. The aminoglycoside antibiotics gentamicin and tobramycin were given to six healthy volunteers by intravenous injection. A dose of 80 mg was injected within 1 min. The curves relating serum concentration to time did not differ significantly for the two drugs. The maximal serum concentrations of drug exceeded 10 mug/ml on seven of 12 occasions. Despite these high concentrations, loss of hearing was not significant at any frequency (1,000-8,000 Hz) up to one month after injection. It is suggested that the aminoglycoside antibiotics penetrate into the inner ear lymph very slowly and that the concentration at that site is relatively unaffected by transient high concentrations of antibiotic in the blood plasma.", "PMID": 972268}
{"id": "pubmed23n0032_8930", "title": "Pharmacokinetics of intravenously administered tobramycin in normal volunteers and in renal-impaired and hemodialyzed patients.", "content": "The pharmacokinetics of intravenously administered tobramycin, an aminoglycoside antibiotic, were studied in 16 volunteers with normal renal function and 10 patients with different degrees of renal impairment. In all subjects, the disappearance of tobramycin from serum followed first-order kinetics, and the elimination rate constant decreased proportionally with increasing degree of renal impairment. The half-life average 82 min in normal subjects (endogenous creatinine clearance, greater than 80 ml/1.73 m2 per min) and was 33.7 hr in a totally anephric subject. Linear relations were defined between the half-life of tobramycin in serum and the reciprocal of the endogenous creatinine clearance and between the half-life of the drug and the concentration of serum creatinine. These relations may be used for dosage adjustment in patients with renal impairment. In hemodialyzed patients, the half-life decreased six- to ninefold during dialysis for 6 hr. The extent of tobramycin elimination by hemodialysis may be predicted from relations established between the elimination of tobramycin and that of creatinine and blood urea nitrogen. Dosage schedules must be compatible with the pharmacologic and pharmacokinetic properties of the antibiotic.", "contents": "Pharmacokinetics of intravenously administered tobramycin in normal volunteers and in renal-impaired and hemodialyzed patients. The pharmacokinetics of intravenously administered tobramycin, an aminoglycoside antibiotic, were studied in 16 volunteers with normal renal function and 10 patients with different degrees of renal impairment. In all subjects, the disappearance of tobramycin from serum followed first-order kinetics, and the elimination rate constant decreased proportionally with increasing degree of renal impairment. The half-life average 82 min in normal subjects (endogenous creatinine clearance, greater than 80 ml/1.73 m2 per min) and was 33.7 hr in a totally anephric subject. Linear relations were defined between the half-life of tobramycin in serum and the reciprocal of the endogenous creatinine clearance and between the half-life of the drug and the concentration of serum creatinine. These relations may be used for dosage adjustment in patients with renal impairment. In hemodialyzed patients, the half-life decreased six- to ninefold during dialysis for 6 hr. The extent of tobramycin elimination by hemodialysis may be predicted from relations established between the elimination of tobramycin and that of creatinine and blood urea nitrogen. Dosage schedules must be compatible with the pharmacologic and pharmacokinetic properties of the antibiotic.", "PMID": 972269}
{"id": "pubmed23n0032_8931", "title": "Administration of tobramycin and gentamicin by the intravenous route every 6 hr in patients with normal renal function.", "content": "The feasibility and safety of administration of tobramycin and gentamicin in every 6 hr rather than every 8 hr was studied in 18 patients with normal renal function. Eleven patients received tobramycin in a dose of 1.0-3.2 mg/kg every 6 hr, and seven received gentamicin in a dose of 1.4-2.0 mg/kg every 6 hr. The mean peak serum concentrations, excluding the levels in the three children given tobramycin, were 5.5 and 5.8 mug/ml in each group, respectively, after maximal dosage; trough levels 2 hr before the next dose were less than or equal to 1.8 mug/ml in both groups of patients. Neither drug accumulated in the serum when the dosage regimen was maintained for three to 19 days. There was no evidence of nephrotoxicity or ototoxicity. Administration of tobramycin and gentamicin every 6 hr seems to be a reasonable program for patients with normal renal function, pending further studies on safety.", "contents": "Administration of tobramycin and gentamicin by the intravenous route every 6 hr in patients with normal renal function. The feasibility and safety of administration of tobramycin and gentamicin in every 6 hr rather than every 8 hr was studied in 18 patients with normal renal function. Eleven patients received tobramycin in a dose of 1.0-3.2 mg/kg every 6 hr, and seven received gentamicin in a dose of 1.4-2.0 mg/kg every 6 hr. The mean peak serum concentrations, excluding the levels in the three children given tobramycin, were 5.5 and 5.8 mug/ml in each group, respectively, after maximal dosage; trough levels 2 hr before the next dose were less than or equal to 1.8 mug/ml in both groups of patients. Neither drug accumulated in the serum when the dosage regimen was maintained for three to 19 days. There was no evidence of nephrotoxicity or ototoxicity. Administration of tobramycin and gentamicin every 6 hr seems to be a reasonable program for patients with normal renal function, pending further studies on safety.", "PMID": 972270}
{"id": "pubmed23n0032_8932", "title": "Pharmacokinetics of tobramycin in rats with reduced renal parenchyma and in experimental shock.", "content": "Ten days after four-fifths nephrectomy in rats (four-fifths of the renal parenchyma removed), the total and renal elimination constants of tobramycin were lowered by one-half. The biological half-life of tobramycin doubled. These results suggest that soon after nephrectomy the residual nephrons are able to increase the rate of excretion of tobramycin. The increase in mass of the remaining renal tissue between day 10 and day 80 after nephrectomy had no effect on the biological half-life, on the total and renal elimination constants, and on the renal clearance of tobramycin. During endotoxin shock and burn shock, concentrations of tobramycin in serum decreased at a slower rate. The highest serum concentrations of tobramycin were observed in rats with shock due to burns.", "contents": "Pharmacokinetics of tobramycin in rats with reduced renal parenchyma and in experimental shock. Ten days after four-fifths nephrectomy in rats (four-fifths of the renal parenchyma removed), the total and renal elimination constants of tobramycin were lowered by one-half. The biological half-life of tobramycin doubled. These results suggest that soon after nephrectomy the residual nephrons are able to increase the rate of excretion of tobramycin. The increase in mass of the remaining renal tissue between day 10 and day 80 after nephrectomy had no effect on the biological half-life, on the total and renal elimination constants, and on the renal clearance of tobramycin. During endotoxin shock and burn shock, concentrations of tobramycin in serum decreased at a slower rate. The highest serum concentrations of tobramycin were observed in rats with shock due to burns.", "PMID": 972271}
{"id": "pubmed23n0032_8933", "title": "Comparison of urinary excretion of tobramycin and gentamicin in adults.", "content": "The urinary excretion of tobramycin and gentamicin was measured after intramuscular injections of 1 mg of each drug/kg in six normal volunteers. The antibiotics were assayed by the adenylyltransferase enzymatic method, which enables urine concentrations to be estimated accurately without dependence on urinary pH. The levels of the antibiotics in urine varied with the urine volume. The concentration of gentamicin was greater than 113 mug/ml and that of tobramycin was greater than 94 mug/ml 1 hr after administration. Urinary excretion of both antibiotics was rapid; 63.5% of the gentamicin and 74% of the tobramycin was excreted with 24 hr, with a large proportion being excreted by 4 hr after injection. There was a striking uniformity of individual values, and the method offers considerable advantages over existing microbiologic assays.", "contents": "Comparison of urinary excretion of tobramycin and gentamicin in adults. The urinary excretion of tobramycin and gentamicin was measured after intramuscular injections of 1 mg of each drug/kg in six normal volunteers. The antibiotics were assayed by the adenylyltransferase enzymatic method, which enables urine concentrations to be estimated accurately without dependence on urinary pH. The levels of the antibiotics in urine varied with the urine volume. The concentration of gentamicin was greater than 113 mug/ml and that of tobramycin was greater than 94 mug/ml 1 hr after administration. Urinary excretion of both antibiotics was rapid; 63.5% of the gentamicin and 74% of the tobramycin was excreted with 24 hr, with a large proportion being excreted by 4 hr after injection. There was a striking uniformity of individual values, and the method offers considerable advantages over existing microbiologic assays.", "PMID": 972272}
{"id": "pubmed23n0032_8934", "title": "Single daily doses of tobramycin in therapy of urinary tract infections.", "content": "Results of therapy with tobramycin for infections caused by susceptible pathogens have been impressive. The usual multiple dose regimen is aimed at maintenance of the serum concentration of tobramycin above the minimal inhibitory concentration of the causative pathogen throughout the course of therapy. Evidence is accumulating, however, that the urinary concentration of antibiotics may be of greater importance than the serum concentration in the treatment of urinary tract infections. This concept has been supported by results of studies with nitrofurantoin, naladixic acid, tetracycline, and, more recently, gentamicin. The present study of 90 patients with urinary tract infections treated with a single intramuscular daily dose of tobramycin resulted in round-the-clock urinary levels of tobramycin that exceeded the minimal inhibitory concentration of the offending pathogen. The clinical and bacteriologic response to therapy was excellent, and no oto- or nephrotoxicity occurred. This regimen frequently allows for therapy on an outpatient basis, fewer painful injections for the patient, and the saving of nursing time and expensive supplies.", "contents": "Single daily doses of tobramycin in therapy of urinary tract infections. Results of therapy with tobramycin for infections caused by susceptible pathogens have been impressive. The usual multiple dose regimen is aimed at maintenance of the serum concentration of tobramycin above the minimal inhibitory concentration of the causative pathogen throughout the course of therapy. Evidence is accumulating, however, that the urinary concentration of antibiotics may be of greater importance than the serum concentration in the treatment of urinary tract infections. This concept has been supported by results of studies with nitrofurantoin, naladixic acid, tetracycline, and, more recently, gentamicin. The present study of 90 patients with urinary tract infections treated with a single intramuscular daily dose of tobramycin resulted in round-the-clock urinary levels of tobramycin that exceeded the minimal inhibitory concentration of the offending pathogen. The clinical and bacteriologic response to therapy was excellent, and no oto- or nephrotoxicity occurred. This regimen frequently allows for therapy on an outpatient basis, fewer painful injections for the patient, and the saving of nursing time and expensive supplies.", "PMID": 972273}
{"id": "pubmed23n0032_8935", "title": "Comparison of tobramycin and gentamicin in the treatment of complicated urinary tract infections.", "content": "The efficacy and safety of intramuscular administration of either of the two aminoglycoside antibiotics tobramycin and gentamicin every 8 hr for seven days were compared in a prospective, randomized study of 75 elderly male patients with complicated urinary tract infections. All bacteria were sensitive to both antibiotics by the disk diffusion method. The two groups of patients (each receiving one of the two drugs) were comparable in terms of infecting microorganisms and underlying pathology of the urinary tract (mostly prostatic hyperplasia or carcinoma and urethral strictures). No patients had indwelling catheters. All patients had normal renal function (serum creatinine, less than or equal to 1.5 mg/100 ml and/or blood urea nitrogen, less than or equal to 25 mg/100 ml). In both groups 64% of the patients were cured of the infection, with cure defined as a negative urine culture result seven days after discontinuation of therapy. A statistically but not clinically significant increase in the level of serum creatinine in both groups of patients was noted after treatment.", "contents": "Comparison of tobramycin and gentamicin in the treatment of complicated urinary tract infections. The efficacy and safety of intramuscular administration of either of the two aminoglycoside antibiotics tobramycin and gentamicin every 8 hr for seven days were compared in a prospective, randomized study of 75 elderly male patients with complicated urinary tract infections. All bacteria were sensitive to both antibiotics by the disk diffusion method. The two groups of patients (each receiving one of the two drugs) were comparable in terms of infecting microorganisms and underlying pathology of the urinary tract (mostly prostatic hyperplasia or carcinoma and urethral strictures). No patients had indwelling catheters. All patients had normal renal function (serum creatinine, less than or equal to 1.5 mg/100 ml and/or blood urea nitrogen, less than or equal to 25 mg/100 ml). In both groups 64% of the patients were cured of the infection, with cure defined as a negative urine culture result seven days after discontinuation of therapy. A statistically but not clinically significant increase in the level of serum creatinine in both groups of patients was noted after treatment.", "PMID": 972274}
{"id": "pubmed23n0032_8936", "title": "Laboratory and clinical evaluation of tobramycin, a new aminoglycoside antibiotic.", "content": "The in vitro susceptibility to tobramycin of 1,466 bacterial isolates, including several genera and strains of common pathogenic gram-negative bacilli and Staphylococcus aureus, was determined by conventional serial dilution methods. Tobramycin exhibited marked in vitro antibacterial activity; almost all of the isolates tested were inhibited by less than or equal to 2.5 mug of the drug/ml. The antibiotic was used in the treatment of several types of infection in 29 patients with normal renal function. Observations were made on the therapeutic and secondary effects of the drug and on possible toxicity to the eighth cranial nerve. Clinical therapeutic results were satisfactory, The hearing of one patient was impaired, but the relation of this effect to tobramycin therapy is uncertain. Serum levels of the antibiotic were measured in 10 patients to investigate whether the drug accumulated during repeated administration. Mean serum levels of tobramycin 1 hr after intramuscular injection of 1.0 mg/kg were between 2.5 and 3.0 mug/ml on days 1, 3, and 6 of therapy. No accumulation of the antibiotic was observed after repeated administration.", "contents": "Laboratory and clinical evaluation of tobramycin, a new aminoglycoside antibiotic. The in vitro susceptibility to tobramycin of 1,466 bacterial isolates, including several genera and strains of common pathogenic gram-negative bacilli and Staphylococcus aureus, was determined by conventional serial dilution methods. Tobramycin exhibited marked in vitro antibacterial activity; almost all of the isolates tested were inhibited by less than or equal to 2.5 mug of the drug/ml. The antibiotic was used in the treatment of several types of infection in 29 patients with normal renal function. Observations were made on the therapeutic and secondary effects of the drug and on possible toxicity to the eighth cranial nerve. Clinical therapeutic results were satisfactory, The hearing of one patient was impaired, but the relation of this effect to tobramycin therapy is uncertain. Serum levels of the antibiotic were measured in 10 patients to investigate whether the drug accumulated during repeated administration. Mean serum levels of tobramycin 1 hr after intramuscular injection of 1.0 mg/kg were between 2.5 and 3.0 mug/ml on days 1, 3, and 6 of therapy. No accumulation of the antibiotic was observed after repeated administration.", "PMID": 972275}
{"id": "pubmed23n0032_8937", "title": "Tobramycin in pediatric use.", "content": "The new aminoglycoside antibiotic, tobramycin, was used for treatment of gram-negative and staphylococcal infection in 38 neonates, infants, and children in a pediatric surgical unit. Levels of drug in serum after administration by intramuscular, intravenous, and intraperitoneal routes were monitored, and control of infections was generally good within the therapeutic range of 2.0-10.0 mug/ml with a standard dosage regimen of approximately 5 mg/kg per day. Impairment of renal function and concurrent lincomycin therapy were important factors causing variation of levels in serum outside this range. Levels of tobramycin in cerebrospinal fluid after intraventricular instillation varied greatly in one patient, but were satisfactory in another. Clinical and bacteriological assessment of results indicated only two failures of treatment, although infection with a known resistant organism supervened in three cases. Screening for renal, hepatic, and hematological toxicity revealed only one case of transient and reversible renal impairment. Response to tobramycin therapy was generally rapid and satisfactory in a group of young patients with moderately severe infections, many of which were complicated by the presence of a congenital anomaly.", "contents": "Tobramycin in pediatric use. The new aminoglycoside antibiotic, tobramycin, was used for treatment of gram-negative and staphylococcal infection in 38 neonates, infants, and children in a pediatric surgical unit. Levels of drug in serum after administration by intramuscular, intravenous, and intraperitoneal routes were monitored, and control of infections was generally good within the therapeutic range of 2.0-10.0 mug/ml with a standard dosage regimen of approximately 5 mg/kg per day. Impairment of renal function and concurrent lincomycin therapy were important factors causing variation of levels in serum outside this range. Levels of tobramycin in cerebrospinal fluid after intraventricular instillation varied greatly in one patient, but were satisfactory in another. Clinical and bacteriological assessment of results indicated only two failures of treatment, although infection with a known resistant organism supervened in three cases. Screening for renal, hepatic, and hematological toxicity revealed only one case of transient and reversible renal impairment. Response to tobramycin therapy was generally rapid and satisfactory in a group of young patients with moderately severe infections, many of which were complicated by the presence of a congenital anomaly.", "PMID": 972276}
{"id": "pubmed23n0032_8938", "title": "Tobramycin and cephalothin sodium in treatment of infected patients with acute leukemia.", "content": "Tobramycin has been shown to be safe and effective when given intravenously in combination antibiotic therapy for infected patients with acute leukemia or other profound pancytopenic states. Tobramycin in combination with one or more antibiotics (usually cephalothin) produced satisfactory clinical results in 21 of 30 episodes of fever. Toxic effects were minimal and were limited to occasional mild thrombophlebitis.", "contents": "Tobramycin and cephalothin sodium in treatment of infected patients with acute leukemia. Tobramycin has been shown to be safe and effective when given intravenously in combination antibiotic therapy for infected patients with acute leukemia or other profound pancytopenic states. Tobramycin in combination with one or more antibiotics (usually cephalothin) produced satisfactory clinical results in 21 of 30 episodes of fever. Toxic effects were minimal and were limited to occasional mild thrombophlebitis.", "PMID": 972277}
{"id": "pubmed23n0032_8939", "title": "Tobramycin and cephalothin for treatment of suspected sepsis in neutropenic children with cancer.", "content": "The safety and efficacy of tobramycin and cephalothin in treatment of suspected sepsis were studied in neutropenic children with various malignancies. Twenty episodes of suspected sepsis in 19 febrile children with cancer were treated with parenteral tobramycin and cephalothin; the duration of therapy ranged from one to 80 days. In 14 of the 20 episodes of suspected sepsis, a favorable clinical response was achieved within five days after initiation of antibiotic therapy. These episodes included a urinary tract infection with Proteus mirabilis and sepsis due to Escherichia coli. In four of the additional six episodes, clinical deterioration was though to be caused by the underlying malignancies. Two episodes included a case of E. coli spesis that ended fatally and a nosocomial infection with Eikenella corrodens. Results of this study suggest that combination therapy with tobramycin and cephalothin is safe and efficacious in treatment of suspected sepsis in febrile children with malignancies and neutropenia.", "contents": "Tobramycin and cephalothin for treatment of suspected sepsis in neutropenic children with cancer. The safety and efficacy of tobramycin and cephalothin in treatment of suspected sepsis were studied in neutropenic children with various malignancies. Twenty episodes of suspected sepsis in 19 febrile children with cancer were treated with parenteral tobramycin and cephalothin; the duration of therapy ranged from one to 80 days. In 14 of the 20 episodes of suspected sepsis, a favorable clinical response was achieved within five days after initiation of antibiotic therapy. These episodes included a urinary tract infection with Proteus mirabilis and sepsis due to Escherichia coli. In four of the additional six episodes, clinical deterioration was though to be caused by the underlying malignancies. Two episodes included a case of E. coli spesis that ended fatally and a nosocomial infection with Eikenella corrodens. Results of this study suggest that combination therapy with tobramycin and cephalothin is safe and efficacious in treatment of suspected sepsis in febrile children with malignancies and neutropenia.", "PMID": 972278}
{"id": "pubmed23n0032_8940", "title": "Clinical use of tobramycin in patients with surgical infections due to gram-negative bacilli.", "content": "Twenty-three patients with acute peritonitis, intraabdominal abscess, wound infections (including infection of serious burns), sepsis accompanying renal transplantation, or urinary tract infection associated with cancer were treated with various dosage regimens of tobramycin, and their clinical responses were analysed. For the conditions studied, the optimal regimen was 3 mg/kg per day in three divided doses. No clinical effectiveness was noted in this study for doses of greater than or equal to 4 mg/kg per day.", "contents": "Clinical use of tobramycin in patients with surgical infections due to gram-negative bacilli. Twenty-three patients with acute peritonitis, intraabdominal abscess, wound infections (including infection of serious burns), sepsis accompanying renal transplantation, or urinary tract infection associated with cancer were treated with various dosage regimens of tobramycin, and their clinical responses were analysed. For the conditions studied, the optimal regimen was 3 mg/kg per day in three divided doses. No clinical effectiveness was noted in this study for doses of greater than or equal to 4 mg/kg per day.", "PMID": 972279}
{"id": "pubmed23n0032_8941", "title": "Clinical evaluation of tobramycin in respiratory and systemic infections in immunodepressed and normal patients.", "content": "Twelve patients with acute or chronic pneumonia due mainly to gram-negative bacilli, two patients with pseudomonas endocarditis, and two patients with seratia sepsis were treated with 80-160 mg of tobramycin in two daily doses. Fourteen infected patients with underlying leukemia or lymphoma received this dose of tobramycin combined with cefazolin or penicillin. Most respiratory infections were cured or markedly improved. with eradication or significant reduction in the number of infecting organisms. One case of pseudomonas endocarditis and both cases of serratia sepsis were also cured. Combined treatment with tobramycin and beta-lactam antibiotics resulted in clinical and bacteriological improvement in 50% of systemic immunodepressed patients with sepsis and/or pneumonia.", "contents": "Clinical evaluation of tobramycin in respiratory and systemic infections in immunodepressed and normal patients. Twelve patients with acute or chronic pneumonia due mainly to gram-negative bacilli, two patients with pseudomonas endocarditis, and two patients with seratia sepsis were treated with 80-160 mg of tobramycin in two daily doses. Fourteen infected patients with underlying leukemia or lymphoma received this dose of tobramycin combined with cefazolin or penicillin. Most respiratory infections were cured or markedly improved. with eradication or significant reduction in the number of infecting organisms. One case of pseudomonas endocarditis and both cases of serratia sepsis were also cured. Combined treatment with tobramycin and beta-lactam antibiotics resulted in clinical and bacteriological improvement in 50% of systemic immunodepressed patients with sepsis and/or pneumonia.", "PMID": 972280}
{"id": "pubmed23n0032_8942", "title": "Tobramycin therapy of infections due to Pseudomonas aeruginosa in patients with cystic fibrosis: effect of dosage and concentration of antibiotic in sputum.", "content": "Established respiratory infections with mucoid Pseudomonas aeruginosa in patients suffering from cystic fibrosis were treated with conventional as well as larger doses of tobramycin. The infection was eradicated in four of the 17 patients treated, but the duration of follow-up study of one patient was short. It appeared that treatment was most successful in those patients in whom the highest peak concentrations of tobramycin in sputum were obtained. No side effects were noted, even when tobramycin was given in large doses for two weeks.", "contents": "Tobramycin therapy of infections due to Pseudomonas aeruginosa in patients with cystic fibrosis: effect of dosage and concentration of antibiotic in sputum. Established respiratory infections with mucoid Pseudomonas aeruginosa in patients suffering from cystic fibrosis were treated with conventional as well as larger doses of tobramycin. The infection was eradicated in four of the 17 patients treated, but the duration of follow-up study of one patient was short. It appeared that treatment was most successful in those patients in whom the highest peak concentrations of tobramycin in sputum were obtained. No side effects were noted, even when tobramycin was given in large doses for two weeks.", "PMID": 972281}
{"id": "pubmed23n0032_8943", "title": "Pharmacokinetics and ototoxicity of gentamicin, tobramycin, and amikacin.", "content": "The pharmacokinetics of gentamicin, tobramycin, and amikacin in inner ear fluids, serum, cerebrospinal fluid, and the compartments of the eye were studied and compared in guinea pigs. The concentrations of antibiotic were determined by microbiologic methods and were confirmed by the use of 14C-labeled gentamicin. Retention was clearly demonstrated in perilymph, in which the half-lives of gentamicin, tobramycin, and amikacin were 12, 11, and 10 hr, respectively. The concentrations of drug in perilymph were symmetrical and were many times higher than the concentrations of antibiotic in the brain. There was no difference between the concentration of drug in endolymph and that in perilymph. A linear relation between concentrations in the perilymph and the dosage of gentamicin was ascertained. Long-term treatment did not influence the pharmacokinetics of the three antibiotics in the inner ear. However, increased levels of drug in the inner ear in animals with uremia and in some animals with otitis media explained the increased ototoxicity that occurs in treatment of these two conditions. Suboccipital puncture and diuresis did not change the concentrations of aminoglycoside antibiotics in the inner ear. Antibiotics applied locally in the middle ear had high degrees of ototoxicity.", "contents": "Pharmacokinetics and ototoxicity of gentamicin, tobramycin, and amikacin. The pharmacokinetics of gentamicin, tobramycin, and amikacin in inner ear fluids, serum, cerebrospinal fluid, and the compartments of the eye were studied and compared in guinea pigs. The concentrations of antibiotic were determined by microbiologic methods and were confirmed by the use of 14C-labeled gentamicin. Retention was clearly demonstrated in perilymph, in which the half-lives of gentamicin, tobramycin, and amikacin were 12, 11, and 10 hr, respectively. The concentrations of drug in perilymph were symmetrical and were many times higher than the concentrations of antibiotic in the brain. There was no difference between the concentration of drug in endolymph and that in perilymph. A linear relation between concentrations in the perilymph and the dosage of gentamicin was ascertained. Long-term treatment did not influence the pharmacokinetics of the three antibiotics in the inner ear. However, increased levels of drug in the inner ear in animals with uremia and in some animals with otitis media explained the increased ototoxicity that occurs in treatment of these two conditions. Suboccipital puncture and diuresis did not change the concentrations of aminoglycoside antibiotics in the inner ear. Antibiotics applied locally in the middle ear had high degrees of ototoxicity.", "PMID": 972282}
{"id": "pubmed23n0032_8944", "title": "Tobramycin adenylyltransferase: a new aminoglycoside-inactivating enzyme from Staphylococcus epidermidis.", "content": "Certain strains of Staphylococcus epidermidis resistant to the aminoglycoside antibiotics were shown to contain an enzyme that inactivates the kanamycins, neomycins, butirosins, paromomycin, gentamicin A, amikacin, and tobramycin by adenylylation. Tobramycin adenylyltransferase, as this enzyme is called, was found to be optimally active at pH 5.5. With paromomycin or neomycin B and C as substrates, however, two pH values (5.5 and 9.0) for optimal activity were observed. The enzyme requires Mg++ for activity and is stabilized significantly by dithiothreitol. It is probable that the 4'-hydroxyl group of ring I of the antibiotics is adenylylated. Those aminoglycosides that are not substrates for the enzyme lack a hydroxyl group in the corresponding position.", "contents": "Tobramycin adenylyltransferase: a new aminoglycoside-inactivating enzyme from Staphylococcus epidermidis. Certain strains of Staphylococcus epidermidis resistant to the aminoglycoside antibiotics were shown to contain an enzyme that inactivates the kanamycins, neomycins, butirosins, paromomycin, gentamicin A, amikacin, and tobramycin by adenylylation. Tobramycin adenylyltransferase, as this enzyme is called, was found to be optimally active at pH 5.5. With paromomycin or neomycin B and C as substrates, however, two pH values (5.5 and 9.0) for optimal activity were observed. The enzyme requires Mg++ for activity and is stabilized significantly by dithiothreitol. It is probable that the 4'-hydroxyl group of ring I of the antibiotics is adenylylated. Those aminoglycosides that are not substrates for the enzyme lack a hydroxyl group in the corresponding position.", "PMID": 972283}
{"id": "pubmed23n0032_8945", "title": "Emergence of gentamicin-resistant bacteria: experience with tobramycin therapy of infections due to gentamicin-resistant organisms.", "content": "A computerized system for testing and surveillance of bacterial susceptibility to antibiotics was used in monitoring the emergence of gentamicin-resistant strains of aerobic and facultative gram-negative bacilli at Massachusetts General Hospital since the release of gentamicin for clinical use in 1971. During the period studied, there was a significant increase in the prevalence of gentamicin-resistant bacteria, particularly among Pseudomonas, Acinetobacter (Herellea), and Proteus and, more recently, among Enterobacter and Klebsiella. Most gentamicin-resistant strains of Pseudomonas aeruginosa and Acinetobacter calcoaceticus var. anitratum (Herellea varginicola) retained susceptibility to tobramycin. Of the other gentamicin-resistant organisms, most were also resistant to tobramycin. Twelve patients with infections caused by gentamicin-resistant organisms were treated with tobramycin. All 12 patients were seriously ill, and all but one had failed to respond to previous therapy with gentamicin. Nine patients responded favorably to tobramycin, and six were cured. P. aeruginosa and A. calcoaceticus var. anitratum were most frequently the infecting organisms in these patients. Patients received tobramycin for three to 42 days; no significant drug-related toxicity was noted. These results emphasize the increasing clinical importance of gentamicin-resistant bacteria and suggest that tobramycin may be effective for treatment of some, but not all, infections caused by gentamicin-resistant bacteria.", "contents": "Emergence of gentamicin-resistant bacteria: experience with tobramycin therapy of infections due to gentamicin-resistant organisms. A computerized system for testing and surveillance of bacterial susceptibility to antibiotics was used in monitoring the emergence of gentamicin-resistant strains of aerobic and facultative gram-negative bacilli at Massachusetts General Hospital since the release of gentamicin for clinical use in 1971. During the period studied, there was a significant increase in the prevalence of gentamicin-resistant bacteria, particularly among Pseudomonas, Acinetobacter (Herellea), and Proteus and, more recently, among Enterobacter and Klebsiella. Most gentamicin-resistant strains of Pseudomonas aeruginosa and Acinetobacter calcoaceticus var. anitratum (Herellea varginicola) retained susceptibility to tobramycin. Of the other gentamicin-resistant organisms, most were also resistant to tobramycin. Twelve patients with infections caused by gentamicin-resistant organisms were treated with tobramycin. All 12 patients were seriously ill, and all but one had failed to respond to previous therapy with gentamicin. Nine patients responded favorably to tobramycin, and six were cured. P. aeruginosa and A. calcoaceticus var. anitratum were most frequently the infecting organisms in these patients. Patients received tobramycin for three to 42 days; no significant drug-related toxicity was noted. These results emphasize the increasing clinical importance of gentamicin-resistant bacteria and suggest that tobramycin may be effective for treatment of some, but not all, infections caused by gentamicin-resistant bacteria.", "PMID": 972284}
{"id": "pubmed23n0032_8946", "title": "Evaluation of tobramycin in severe urinary tract infection.", "content": "Twenty-five patients with severe urinary tract infections were treated with 3 mg of tobramycin/kg per day (given in two doses). Susceptible organisms were Escherichia coli, Proteus, Klebsiella, Pseudomonas, Streptococcus, and enterococcus. Clincal conditions in which treatment produced excellent bacteriological results included a wide range of urological disorders; the most common were pyelonephritis, cystitis, and epididymo-orchitis, Three patients had septicemia, and 12 had an infection that was the result of urinary tract obstruction requiring surgery.", "contents": "Evaluation of tobramycin in severe urinary tract infection. Twenty-five patients with severe urinary tract infections were treated with 3 mg of tobramycin/kg per day (given in two doses). Susceptible organisms were Escherichia coli, Proteus, Klebsiella, Pseudomonas, Streptococcus, and enterococcus. Clincal conditions in which treatment produced excellent bacteriological results included a wide range of urological disorders; the most common were pyelonephritis, cystitis, and epididymo-orchitis, Three patients had septicemia, and 12 had an infection that was the result of urinary tract obstruction requiring surgery.", "PMID": 972285}
{"id": "pubmed23n0032_8947", "title": "Effects of electric-shock delivery on schedule-induced water intake: delay of shock, shock intensity, and body-weight loss.", "content": "In each of four experiments, schedule-induced water intake in the rat was studied under fixed-time 40-sec food delivery. Experiments I and II studied the temporal relationship between response-independent electric-shock delivery and licking. Shock was delivered under a variable-time 60-sec schedule. A lick-dependent delay was imposed so that licking and shock delivery were systematically separated in time by a minimum of 1 to 15 sec. Over a wide range of shock intensities the data failed to reveal a consistent delay-of-shock effect. Similar shock intensities led to similar reduction of water intake at each delay of shock interval. Experiments III and IV studied the effects of body-weight loss on water intake during independent shock delivery. In Experiment III, shock was delivered under variable-time 60-sec with a minimum separation between shock and licking of 5 sec. In Experiment IV, shock was delivered under variable-time 180-sec. The minimum separation between shock and licking was 10 sec. In each study, the resistance of water intake to suppression by shock delivery increased as the degree of body-weight loss increased. Schedule-induced water intake was affected more by shock when the animal was maintained at 90% of free-feeding weight than at 70%.", "contents": "Effects of electric-shock delivery on schedule-induced water intake: delay of shock, shock intensity, and body-weight loss. In each of four experiments, schedule-induced water intake in the rat was studied under fixed-time 40-sec food delivery. Experiments I and II studied the temporal relationship between response-independent electric-shock delivery and licking. Shock was delivered under a variable-time 60-sec schedule. A lick-dependent delay was imposed so that licking and shock delivery were systematically separated in time by a minimum of 1 to 15 sec. Over a wide range of shock intensities the data failed to reveal a consistent delay-of-shock effect. Similar shock intensities led to similar reduction of water intake at each delay of shock interval. Experiments III and IV studied the effects of body-weight loss on water intake during independent shock delivery. In Experiment III, shock was delivered under variable-time 60-sec with a minimum separation between shock and licking of 5 sec. In Experiment IV, shock was delivered under variable-time 180-sec. The minimum separation between shock and licking was 10 sec. In each study, the resistance of water intake to suppression by shock delivery increased as the degree of body-weight loss increased. Schedule-induced water intake was affected more by shock when the animal was maintained at 90% of free-feeding weight than at 70%.", "PMID": 972298}
{"id": "pubmed23n0032_8948", "title": "A national assessment of propoxyphene in postmortem medicolegal investigation, 1972-1975.", "content": "Eighteen medical examiners, coroners, and forensic science laboratories and offices, representing a total jurisdictional population of 52.6 million, were visited during November 1975, and more than 1200 cases occurring in the four years from 1972 through 31 July 1975 were evaluated for inclusion in the study. The sites were distributed across the United States and Ontario, Canada, and included urban and rural, states, city, and county jurisdictions. Scientific data and circumstantial information was gathered consistently for each case and site by means of five questionnaires. Finally, 1022 cases were compiled and examined, and the data were analyzed to form the body of data from which this report is written. The following conclusions may be drawn. 1. The number of deaths involving propoxyphene is increasing each year, and at a faster rate than total drug deaths. The absolute numbers and rate are different in urban and rural areas, but the frequency reached 6.0 deaths per million population in 1974. Deaths attributed to suicide as well as those determined to be accidental deaths and undetermined have increased. 2. Approximately 66% of all the cases studied had the word propoxyphene included in the cause of death statement on the death certificate. Approximately 46% of the cases were classified as suicide (64% of them female and 36% male), 26% as accidents, and 21% as undetermined. 3. The deceased were mainly middle-class, Caucasian, urban dwellers, with male and female evenly distributed. Their ages were from 20 to 50 years, with few outside this range. Female ages were uniformly distributed, but males in their early twenties were very prominent. This is different from the U.S. population age distribution, which is currently dominated by teenagers. Propoxyphene does not appear to be a pediatric problem, as seen in the study. 4. The deceased were not part of the illegal drug abuse population and had no particular propensity for the use of heroin or narcotics, but rather they were a particular medical population of those who misuse prescription drugs and alcohol. 5. The deceased did have a marked tendency to hypochondria, chronic minor illnesses and emotional problems. Some 43% had recent medical histories, and 82% had a documented psychiatric history which often included (51%) self-destructuve behavior such as suicide attempts. Almost all received a wide range of prescription drugs, particularly tranquilizers, which they often misused in the sense of self-medicating, multiple drug ingestion, and combining alcohol with their medication. Approximately 34% had a history of misusing some drug, and 20% could be defined as abusers in that they were prone to excessive use of their medications; 44% had diazepam available to them, and 17% were either problem drinkers or alcoholics.", "contents": "A national assessment of propoxyphene in postmortem medicolegal investigation, 1972-1975. Eighteen medical examiners, coroners, and forensic science laboratories and offices, representing a total jurisdictional population of 52.6 million, were visited during November 1975, and more than 1200 cases occurring in the four years from 1972 through 31 July 1975 were evaluated for inclusion in the study. The sites were distributed across the United States and Ontario, Canada, and included urban and rural, states, city, and county jurisdictions. Scientific data and circumstantial information was gathered consistently for each case and site by means of five questionnaires. Finally, 1022 cases were compiled and examined, and the data were analyzed to form the body of data from which this report is written. The following conclusions may be drawn. 1. The number of deaths involving propoxyphene is increasing each year, and at a faster rate than total drug deaths. The absolute numbers and rate are different in urban and rural areas, but the frequency reached 6.0 deaths per million population in 1974. Deaths attributed to suicide as well as those determined to be accidental deaths and undetermined have increased. 2. Approximately 66% of all the cases studied had the word propoxyphene included in the cause of death statement on the death certificate. Approximately 46% of the cases were classified as suicide (64% of them female and 36% male), 26% as accidents, and 21% as undetermined. 3. The deceased were mainly middle-class, Caucasian, urban dwellers, with male and female evenly distributed. Their ages were from 20 to 50 years, with few outside this range. Female ages were uniformly distributed, but males in their early twenties were very prominent. This is different from the U.S. population age distribution, which is currently dominated by teenagers. Propoxyphene does not appear to be a pediatric problem, as seen in the study. 4. The deceased were not part of the illegal drug abuse population and had no particular propensity for the use of heroin or narcotics, but rather they were a particular medical population of those who misuse prescription drugs and alcohol. 5. The deceased did have a marked tendency to hypochondria, chronic minor illnesses and emotional problems. Some 43% had recent medical histories, and 82% had a documented psychiatric history which often included (51%) self-destructuve behavior such as suicide attempts. Almost all received a wide range of prescription drugs, particularly tranquilizers, which they often misused in the sense of self-medicating, multiple drug ingestion, and combining alcohol with their medication. Approximately 34% had a history of misusing some drug, and 20% could be defined as abusers in that they were prone to excessive use of their medications; 44% had diazepam available to them, and 17% were either problem drinkers or alcoholics.", "PMID": 972299}
{"id": "pubmed23n0032_8949", "title": "A precipitin-inhibition test on denatured bloodstains for the determination of human origin.", "content": "A new method has been developed for determination of the species of origin of denatured bloodstains. Antiserum with known titer is incubated with the denatured bloodstain. If the bloodstain has the same origin as the antiserum, a substantial amount of antibody will be absorbed by the stain, resulting in an attenuation of the antiserum titer. By reacting the supernatant with a known serum by using immunodiffusion it is possible to detect any appreciable attenuation of the antiserum titer. We have been able to determine the species of origin of various denatured bloodstains using this inhibition method.", "contents": "A precipitin-inhibition test on denatured bloodstains for the determination of human origin. A new method has been developed for determination of the species of origin of denatured bloodstains. Antiserum with known titer is incubated with the denatured bloodstain. If the bloodstain has the same origin as the antiserum, a substantial amount of antibody will be absorbed by the stain, resulting in an attenuation of the antiserum titer. By reacting the supernatant with a known serum by using immunodiffusion it is possible to detect any appreciable attenuation of the antiserum titer. We have been able to determine the species of origin of various denatured bloodstains using this inhibition method.", "PMID": 972308}
{"id": "pubmed23n0032_8950", "title": "Detection of amphetamine and methamphetamine-type materials in pharmaceutical and biological fluids by fluorometric labeling.", "content": "A rapid and sensitive method for detecting amphetamine and methamphetamine in drug preparations and biological fluids has been developed. Amphetamine and methamphetamine in pharmaceutical and clandestine drug preparations can be easily screened from other contaminating drugs and readily identified by their fluorescence, with subsequent separation accomplished by TLC. The same general procedure can also be used to detect amphetamine and methamphetamine in human urine at concentrations of 0.1 mug/ml.", "contents": "Detection of amphetamine and methamphetamine-type materials in pharmaceutical and biological fluids by fluorometric labeling. A rapid and sensitive method for detecting amphetamine and methamphetamine in drug preparations and biological fluids has been developed. Amphetamine and methamphetamine in pharmaceutical and clandestine drug preparations can be easily screened from other contaminating drugs and readily identified by their fluorescence, with subsequent separation accomplished by TLC. The same general procedure can also be used to detect amphetamine and methamphetamine in human urine at concentrations of 0.1 mug/ml.", "PMID": 972312}
{"id": "pubmed23n0032_8951", "title": "Several unusual cases of child abuse.", "content": "All childhood deaths which occurred in New Mexico during 1974 and 1975 were reviewed. Nine fatal instances of abuse were identified representing the entire spectrum of physical abuse: neglect, abuse in a single episode of injury, repetitive abuse, or sexual abuse. Several cases are summarized. These are unusual either in the distribution of pathologic findings or in the problems encountered in court presentation.", "contents": "Several unusual cases of child abuse. All childhood deaths which occurred in New Mexico during 1974 and 1975 were reviewed. Nine fatal instances of abuse were identified representing the entire spectrum of physical abuse: neglect, abuse in a single episode of injury, repetitive abuse, or sexual abuse. Several cases are summarized. These are unusual either in the distribution of pathologic findings or in the problems encountered in court presentation.", "PMID": 972313}
{"id": "pubmed23n0032_8952", "title": "Short-range ammunition--a possible anti-hijacking device.", "content": "Short-range ammunition was developed for use by law enforcement personnel in congested, enclosed areas and primarily as a hijacking deterrent in commercial airliners. This ammunition was expected to reduce the risk of injury to innocent bystanders and prevent damage to the aircraft walls. Experimentally, short-range ammunition is effective in its wounding capacity at close ranges, and it does not exit from the body. Due to the particular structure of the projectile, injuries due to ricochet are averted. At ranges of 6 ft (1.8 m), the risk of damage to the full thickness of an aircraft wall does exist. Short-range ammunition deserves serious consideration as an alternative to regular ammunition for use in specialized, close-quartered situations, such as hijacking.", "contents": "Short-range ammunition--a possible anti-hijacking device. Short-range ammunition was developed for use by law enforcement personnel in congested, enclosed areas and primarily as a hijacking deterrent in commercial airliners. This ammunition was expected to reduce the risk of injury to innocent bystanders and prevent damage to the aircraft walls. Experimentally, short-range ammunition is effective in its wounding capacity at close ranges, and it does not exit from the body. Due to the particular structure of the projectile, injuries due to ricochet are averted. At ranges of 6 ft (1.8 m), the risk of damage to the full thickness of an aircraft wall does exist. Short-range ammunition deserves serious consideration as an alternative to regular ammunition for use in specialized, close-quartered situations, such as hijacking.", "PMID": 972314}
{"id": "pubmed23n0032_8953", "title": "Studies of the spatial distribution of firearms discharge residues.", "content": "Fingerprint lifting tape has been applied to the lifting of firearms discharge residue from various surfaces. Lifting efficiencies from coarse fabric ranged from 30 to 70%. Use of the technique has been demonstrated in identifying a bullet hole and in measuring residue patterns on floor and firing hand surfaces. In hand lifts it has been shown that autoradiography alone does not uniquely distinguish the hand of someone who has fired a revolver from the hand of someone who has not. Neutron activation analysis of lifts from selected hand areas may, however, provide such a distinction.", "contents": "Studies of the spatial distribution of firearms discharge residues. Fingerprint lifting tape has been applied to the lifting of firearms discharge residue from various surfaces. Lifting efficiencies from coarse fabric ranged from 30 to 70%. Use of the technique has been demonstrated in identifying a bullet hole and in measuring residue patterns on floor and firing hand surfaces. In hand lifts it has been shown that autoradiography alone does not uniquely distinguish the hand of someone who has fired a revolver from the hand of someone who has not. Neutron activation analysis of lifts from selected hand areas may, however, provide such a distinction.", "PMID": 972316}
{"id": "pubmed23n0032_8954", "title": "Programmed energy dispersive X-ray analysis of top coats of automotive paint.", "content": "Elemental, qualitative, and semiquantitative analyses of 1974 U.S. automotive paints by a Finnigan Model 900F Energy Dispersive X-ray (EDX) Spectrometer are examined. This EDX analytical system incorporates a minicomputer to receive, store, and present data. The paint samples were received from the National Bureau of Standards (NBS) Law Enforcement Standards Laboratory as a collection representative of the top coats used on all U.S. manufactured automobiles in 1974. Aspects considered include EDX system stability, homogeneity of similarly catalogued samples from different NBS collections, categorization of paints by predominant constituents, and comparison of data on paint standards that were similar. Automated analysis represents an important application of forensic science to the characterization of paint samples and provides for the nondestructive analysis of a variety of materials in a large range of sample sizes and concentrations.", "contents": "Programmed energy dispersive X-ray analysis of top coats of automotive paint. Elemental, qualitative, and semiquantitative analyses of 1974 U.S. automotive paints by a Finnigan Model 900F Energy Dispersive X-ray (EDX) Spectrometer are examined. This EDX analytical system incorporates a minicomputer to receive, store, and present data. The paint samples were received from the National Bureau of Standards (NBS) Law Enforcement Standards Laboratory as a collection representative of the top coats used on all U.S. manufactured automobiles in 1974. Aspects considered include EDX system stability, homogeneity of similarly catalogued samples from different NBS collections, categorization of paints by predominant constituents, and comparison of data on paint standards that were similar. Automated analysis represents an important application of forensic science to the characterization of paint samples and provides for the nondestructive analysis of a variety of materials in a large range of sample sizes and concentrations.", "PMID": 972317}
{"id": "pubmed23n0032_8955", "title": "Examination of paints by trace element analysis.", "content": "Trace element analysis using neutron activation analysis is effective and valuable, particularly in the examination of white household paint. Although physical appearance and resin composition are generally similar in these paint samples, trace element composition provides an effective way of distinguishing among them. In the case of automobile paint samples, NAA serves as an important additional technique for discrimination. The technique is important when sample sizes are very small. The technique developed takes a few minutes for sample preparation, a few hours of irradiation time (during which the examiner's presence is not required), and then a few minutes for counting and obtaining quantitative multielement concentration patterns. A technician can easily handle 30 to 50 samples per day.", "contents": "Examination of paints by trace element analysis. Trace element analysis using neutron activation analysis is effective and valuable, particularly in the examination of white household paint. Although physical appearance and resin composition are generally similar in these paint samples, trace element composition provides an effective way of distinguishing among them. In the case of automobile paint samples, NAA serves as an important additional technique for discrimination. The technique is important when sample sizes are very small. The technique developed takes a few minutes for sample preparation, a few hours of irradiation time (during which the examiner's presence is not required), and then a few minutes for counting and obtaining quantitative multielement concentration patterns. A technician can easily handle 30 to 50 samples per day.", "PMID": 972318}
{"id": "pubmed23n0032_8956", "title": "Forensic odontology in missing persons cases.", "content": "Forensic odontology can provide a service early in cases of missing persons where foul play is suspected. The combined effort of the professional police investigator and the odontologist prior to the discovery of an unidentified remains allows for an efficient, accurate identification procedure. The forensic dental interview can provide a working format or starting point in developing a planned interrogation procedure concerning dental information. In such a manner, the planned interview can enhance the investigator's efforts in missing persons cases and other cases requiring dental identification.", "contents": "Forensic odontology in missing persons cases. Forensic odontology can provide a service early in cases of missing persons where foul play is suspected. The combined effort of the professional police investigator and the odontologist prior to the discovery of an unidentified remains allows for an efficient, accurate identification procedure. The forensic dental interview can provide a working format or starting point in developing a planned interrogation procedure concerning dental information. In such a manner, the planned interview can enhance the investigator's efforts in missing persons cases and other cases requiring dental identification.", "PMID": 972323}
{"id": "pubmed23n0032_8957", "title": "Evoked response and kinesthetic measures of augmenting/reducing in schizophrenics: replications and extensions.", "content": "This study represents an attempt to replicate the central results of earlier work on stimulus intensity control. It uses a diverse sample of 40 hospitalized schizophrenic subjects, both chronic and acute. Two measures of stimulus intensity control were used: the Petrie kinesthetic figural aftereffects procedure (KFA), and the EEG method of average evoked response (AER). Finding the earlier results generally applicable, it presents a model of the function of stimulus intensity control in schizophrenia, which suggests that acute schizophrenics are particularly vulnerable to being inundated by stimuli, and therefore, that in order to protect themselves, they tend to reduce the perceived intensity of stimuli. Chronic schizophreniics have higher AER amplitudes and reduce less than acutes, suggesting that among chronics, the reducing mechanism either never was present or failed, whereas paranoid schizophrenics tend to be augmenters--a response style consistent with vigilant scanning.", "contents": "Evoked response and kinesthetic measures of augmenting/reducing in schizophrenics: replications and extensions. This study represents an attempt to replicate the central results of earlier work on stimulus intensity control. It uses a diverse sample of 40 hospitalized schizophrenic subjects, both chronic and acute. Two measures of stimulus intensity control were used: the Petrie kinesthetic figural aftereffects procedure (KFA), and the EEG method of average evoked response (AER). Finding the earlier results generally applicable, it presents a model of the function of stimulus intensity control in schizophrenia, which suggests that acute schizophrenics are particularly vulnerable to being inundated by stimuli, and therefore, that in order to protect themselves, they tend to reduce the perceived intensity of stimuli. Chronic schizophreniics have higher AER amplitudes and reduce less than acutes, suggesting that among chronics, the reducing mechanism either never was present or failed, whereas paranoid schizophrenics tend to be augmenters--a response style consistent with vigilant scanning.", "PMID": 972324}
{"id": "pubmed23n0032_8958", "title": "MDA-assisted psychotherapy with neurotic outpatients: a pilot study.", "content": "Ten neurotic patients (five males and five females) were treated over a period of 2 to 6 months (mean, 4.1) as outpatients. The study allowed for a maximum of 75 hours of psychotherapy (mean, 51.55 hours). During the course of treatment, two to four (mean, 3.5) administrations of MDA (3,4-methylenedioxyamphetamine) were employed as adjunctive aids in an effort to enhance the psychotherapeutic process. The mean duration of the drug sessions was 8 hours (range, 6 to 14 hours). The first administration of MDA took place when, in the therapist's judgment, sufficient rapport had been established with the patient. All patients received an initial dose of 75 mg of MDA; subsequent dosage was allowed to range up to 200 mg. On these occasions, the drug appeared to be well tolerated with no serious side effects or complications observed. Psychometric assessments were obtained pre- and post-treatment, employing the Minnesota Multiphasic Personality Inventory (MMPI), Wittenborn Psychiatric Rating Scales (WPRS), and Brief Psychiatric Rating Scale (BPRS). In addition, follow-up evaluations were obtained 6 months after the termination of therapy by the use of the MMPI, WPRS, BPRS, and a Social History Questionnaire (SHQ) which had also been administered before treatment was initiated. Clinically, the impression was obtained that psychotherapy and the adjunctive use of MDA appeared to facilitate improvement in these patients. This impression was substantiated by significant reductions in scores on the psychometric assessments measuring depression, anxiety, and obsessive-compulsive traits. The meaures evaluating the sense of well-being and self-actualization also were encouraging. Although some of the patients were not as responsive as others, there were no observations to suggest that the condition of any of these patients had become worse.", "contents": "MDA-assisted psychotherapy with neurotic outpatients: a pilot study. Ten neurotic patients (five males and five females) were treated over a period of 2 to 6 months (mean, 4.1) as outpatients. The study allowed for a maximum of 75 hours of psychotherapy (mean, 51.55 hours). During the course of treatment, two to four (mean, 3.5) administrations of MDA (3,4-methylenedioxyamphetamine) were employed as adjunctive aids in an effort to enhance the psychotherapeutic process. The mean duration of the drug sessions was 8 hours (range, 6 to 14 hours). The first administration of MDA took place when, in the therapist's judgment, sufficient rapport had been established with the patient. All patients received an initial dose of 75 mg of MDA; subsequent dosage was allowed to range up to 200 mg. On these occasions, the drug appeared to be well tolerated with no serious side effects or complications observed. Psychometric assessments were obtained pre- and post-treatment, employing the Minnesota Multiphasic Personality Inventory (MMPI), Wittenborn Psychiatric Rating Scales (WPRS), and Brief Psychiatric Rating Scale (BPRS). In addition, follow-up evaluations were obtained 6 months after the termination of therapy by the use of the MMPI, WPRS, BPRS, and a Social History Questionnaire (SHQ) which had also been administered before treatment was initiated. Clinically, the impression was obtained that psychotherapy and the adjunctive use of MDA appeared to facilitate improvement in these patients. This impression was substantiated by significant reductions in scores on the psychometric assessments measuring depression, anxiety, and obsessive-compulsive traits. The meaures evaluating the sense of well-being and self-actualization also were encouraging. Although some of the patients were not as responsive as others, there were no observations to suggest that the condition of any of these patients had become worse.", "PMID": 972325}
{"id": "pubmed23n0032_8959", "title": "The neuropsychological, psychiatric, and physical effects of prolonged and severe stress: 30 years later.", "content": "This study investigates the long term or residual effects resulting from severe and extended exposure to stress. The samples were prisoners of war who were intended in Japan (high stress group) or Europe (low stress group) during the Second World War. They were examined in the following three areas: neuropsychological, psychiatric, and physical/neurological. Significant differences were found in all three spheres between the two groups. Second, to examine further the effect of length of internment on these variables, the low stress group was divided into long term and short term internment duration groups, and then the three groups were compared. Significant differences were found among these three groups. These results are discussed in relation to a traditional model of explanation.", "contents": "The neuropsychological, psychiatric, and physical effects of prolonged and severe stress: 30 years later. This study investigates the long term or residual effects resulting from severe and extended exposure to stress. The samples were prisoners of war who were intended in Japan (high stress group) or Europe (low stress group) during the Second World War. They were examined in the following three areas: neuropsychological, psychiatric, and physical/neurological. Significant differences were found in all three spheres between the two groups. Second, to examine further the effect of length of internment on these variables, the low stress group was divided into long term and short term internment duration groups, and then the three groups were compared. Significant differences were found among these three groups. These results are discussed in relation to a traditional model of explanation.", "PMID": 972326}
{"id": "pubmed23n0032_8960", "title": "A comparative evaluation of two day hospitals. Goal attainment scaling of behavior therapy vs. milieu therapy.", "content": "Previous attempts at program evaluation of partial hospitalization programs have consisted largely of descriptive and anecdotal reports of programs and patient characteristics. There have been no comparative or experimental studies of the effectiveness of differing day treatment programs. In the current study, Goal Attainment Scaling, a goal-oriented program evaluation system, was used with 56 randomly selected partial hospitalization patients who attended two day hospitals: one program was based on behavioral-educational methods, and the other was an eclectic program based on the concepts of a therapeutic community. Patients attending the behavioral-educational program showed greater attainment of their therapeutic goals than did the patients involved in the eclectic program. The greater effectiveness of the behavioral-educational day program increased from the 3-month follow-up to the 2-year follow-up.", "contents": "A comparative evaluation of two day hospitals. Goal attainment scaling of behavior therapy vs. milieu therapy. Previous attempts at program evaluation of partial hospitalization programs have consisted largely of descriptive and anecdotal reports of programs and patient characteristics. There have been no comparative or experimental studies of the effectiveness of differing day treatment programs. In the current study, Goal Attainment Scaling, a goal-oriented program evaluation system, was used with 56 randomly selected partial hospitalization patients who attended two day hospitals: one program was based on behavioral-educational methods, and the other was an eclectic program based on the concepts of a therapeutic community. Patients attending the behavioral-educational program showed greater attainment of their therapeutic goals than did the patients involved in the eclectic program. The greater effectiveness of the behavioral-educational day program increased from the 3-month follow-up to the 2-year follow-up.", "PMID": 972327}
{"id": "pubmed23n0032_8961", "title": "The effects of cerebral electric therapy (electrosleep) on anxiety, depression, and hostility in psychiatric patients.", "content": "The effects of cerebral electric therapy (electrosleep) and placebo on self-ratings of anxiety, depression, and hostility were compared. No difference in the amount of improvement shown by the two groups appeared on any of the five measures employed, although a major placebo effect was evident on all of the criteria. The results argue against the use of electrosleep as a treatment for these symptoms except when it may be employed for its placebo value.", "contents": "The effects of cerebral electric therapy (electrosleep) on anxiety, depression, and hostility in psychiatric patients. The effects of cerebral electric therapy (electrosleep) and placebo on self-ratings of anxiety, depression, and hostility were compared. No difference in the amount of improvement shown by the two groups appeared on any of the five measures employed, although a major placebo effect was evident on all of the criteria. The results argue against the use of electrosleep as a treatment for these symptoms except when it may be employed for its placebo value.", "PMID": 972328}
{"id": "pubmed23n0032_8962", "title": "How long does chlorpromazine last?", "content": "How long does chlorpromazine last? This question regarding the persistence of chlorpromazine (CPZ) in chronically medicated schizophrenic patients after drug discontinuation led to wide ranging preliminary answers. These varied from a few to several days for blood studies up to many months for urinary and clinical studies. At least two implications of the question need to be considered: a) the pharmacological persistence of active drug and/or metabolites after drug discontinuation; and b) the persistence of the therapeutic effects regardless of whether or not active drug and/or metabolites are pharmacologically present. For example, a patient's behavior may improve while on CPZ and this improvement may persist after the active drug and/or metabolites cease to be present in the patient's body. These two areas of inquiry were examined by looking at blood, urinary, and clinical data. Although blood studies undoubtedly give the most definitive data, they are greatly complicated by the lack of definitive information regarding the active moiety (moieties) and crucial sites of action, the large number of metabolites (up to 150 or so), the minute quantities involved (ng/ml), the wide inter- and intrapatient variations, and the newness and lack of complete comparability of the quantitative methodologies. Within these limitations, there are a number of studies that are fairly consistent in showing a half-life of disappearance from the plasma of CPZ and/or metabolites in the range of a few to several hours. This would usually mean that most of the drug and metabolites are cleared from the plasma in a few days after drug discontinuation. Urinary studies are related less directly than blood studies to desired clinical effects. Under steady-state conditions in various studies, 43 to 63 per cent of a daily therapeutic dose of CPZ can be recovered in the urine in 24 hours. After drug discontinuation, urinary drug and/or metabolites in most studies last from about 3 to 18 days, with sometimes minimal or trace amounts after this. The clinical studies show a continuation of therapeutic benefits for up to 6 months and longer in some studies, but there are a number of studies showing placebo (withdrawn) groups deteriorating significantly more than continued drug groups much before this, even as early as 1 to 2 weeks off drug. This examination of the literature tends toward a duration of substantial pharmacological (therapeutic) action of CPZ of no more than a few days after drug discontinuation. For a small number of patients, clinical deterioration begins about the same time, whereas in many others, clinical improvement lasts weeks or months. Some of this latter continuation of improvement is likely not due to CPZ and/or metabolites currently active. There do remain many unanswered questions regarding the persistence of minute amounts of CPZ and/or metabolites in storage and possibly at active sites, and whether or not in some patients this makes a significant contribution.", "contents": "How long does chlorpromazine last? How long does chlorpromazine last? This question regarding the persistence of chlorpromazine (CPZ) in chronically medicated schizophrenic patients after drug discontinuation led to wide ranging preliminary answers. These varied from a few to several days for blood studies up to many months for urinary and clinical studies. At least two implications of the question need to be considered: a) the pharmacological persistence of active drug and/or metabolites after drug discontinuation; and b) the persistence of the therapeutic effects regardless of whether or not active drug and/or metabolites are pharmacologically present. For example, a patient's behavior may improve while on CPZ and this improvement may persist after the active drug and/or metabolites cease to be present in the patient's body. These two areas of inquiry were examined by looking at blood, urinary, and clinical data. Although blood studies undoubtedly give the most definitive data, they are greatly complicated by the lack of definitive information regarding the active moiety (moieties) and crucial sites of action, the large number of metabolites (up to 150 or so), the minute quantities involved (ng/ml), the wide inter- and intrapatient variations, and the newness and lack of complete comparability of the quantitative methodologies. Within these limitations, there are a number of studies that are fairly consistent in showing a half-life of disappearance from the plasma of CPZ and/or metabolites in the range of a few to several hours. This would usually mean that most of the drug and metabolites are cleared from the plasma in a few days after drug discontinuation. Urinary studies are related less directly than blood studies to desired clinical effects. Under steady-state conditions in various studies, 43 to 63 per cent of a daily therapeutic dose of CPZ can be recovered in the urine in 24 hours. After drug discontinuation, urinary drug and/or metabolites in most studies last from about 3 to 18 days, with sometimes minimal or trace amounts after this. The clinical studies show a continuation of therapeutic benefits for up to 6 months and longer in some studies, but there are a number of studies showing placebo (withdrawn) groups deteriorating significantly more than continued drug groups much before this, even as early as 1 to 2 weeks off drug. This examination of the literature tends toward a duration of substantial pharmacological (therapeutic) action of CPZ of no more than a few days after drug discontinuation. For a small number of patients, clinical deterioration begins about the same time, whereas in many others, clinical improvement lasts weeks or months. Some of this latter continuation of improvement is likely not due to CPZ and/or metabolites currently active. There do remain many unanswered questions regarding the persistence of minute amounts of CPZ and/or metabolites in storage and possibly at active sites, and whether or not in some patients this makes a significant contribution.", "PMID": 972329}
{"id": "pubmed23n0032_8963", "title": "A depressive syndrome responsive to lithium. An analysis of 20 cases.", "content": "The authors analyze a series of 20 patients seen over the past 4 years who have shown a dramatic improvement following the introduction of lithium carbonate to their therapy. The results indicate that these patients showed a consistent syndrome with the following features: a) anergic endogenous depression; b) positive family history in first degree probands; c) obsessional personality traits and symptoms; d) hypochondriasis and somatic symptoms; e) failure to respond to previous antidepressant therapy with tricyclic and MAOI compounds as well as ECT. A previous study by Gittleson showed that one third of a series of psychotic depressives admitted to the Maudsley Hospital, London, also displayed obsessional symptoms and hypochondriasis. These patients, however, seemed to do as well with standard antidepressant treatment as a control group of psychotic depressives without obsessional features. However, in this series, there was a 7 per cent residue whose obsessional symptoms worsened, even after recovery from their depression. The authors' group of patients represented approximately 3 per cent of all psychotic depressives seen over the 4-year period and could, therefore, coincide with Gittleson's residue. The mean age of onset of illness in the authors' depressive group was 45.5 years, and this finding, coupled with the high incidence of psychotic depression in first degree relatives, indicates that these patients were suffering from a psychotic depression modified by personality traits, rather than an atypical obsessional neurosis. The consistency of clinical features and specificity of response to lithium therapy appear to indicate that this is a clearly definable clinical syndrome worthy of further investigation.", "contents": "A depressive syndrome responsive to lithium. An analysis of 20 cases. The authors analyze a series of 20 patients seen over the past 4 years who have shown a dramatic improvement following the introduction of lithium carbonate to their therapy. The results indicate that these patients showed a consistent syndrome with the following features: a) anergic endogenous depression; b) positive family history in first degree probands; c) obsessional personality traits and symptoms; d) hypochondriasis and somatic symptoms; e) failure to respond to previous antidepressant therapy with tricyclic and MAOI compounds as well as ECT. A previous study by Gittleson showed that one third of a series of psychotic depressives admitted to the Maudsley Hospital, London, also displayed obsessional symptoms and hypochondriasis. These patients, however, seemed to do as well with standard antidepressant treatment as a control group of psychotic depressives without obsessional features. However, in this series, there was a 7 per cent residue whose obsessional symptoms worsened, even after recovery from their depression. The authors' group of patients represented approximately 3 per cent of all psychotic depressives seen over the 4-year period and could, therefore, coincide with Gittleson's residue. The mean age of onset of illness in the authors' depressive group was 45.5 years, and this finding, coupled with the high incidence of psychotic depression in first degree relatives, indicates that these patients were suffering from a psychotic depression modified by personality traits, rather than an atypical obsessional neurosis. The consistency of clinical features and specificity of response to lithium therapy appear to indicate that this is a clearly definable clinical syndrome worthy of further investigation.", "PMID": 972330}
{"id": "pubmed23n0032_8964", "title": "Psychiatric diagnosis and tricyclic plasma levels in 36 hospitalized overdose patients.", "content": "Thirty-six hospitalized patients who were considered by emergency room physicians to have ingested medically serious tricyclic overdoses were studied. The purpose of the project was to determine if psychiatric diagnosis and associated psychiatric factors correlated with the severity of the overdose as defined by plasma drug levels. Medically serious overdoses were ingested by patients with alcoholism, primary affective disorder, undiagnosed psychiatric illness, and Briquet's syndrome. Prior psychiatric treatment, prior admissions, prior overdoses, or precipitating events did not correlate with the medical severity of the ingestion. Attempting to predict the individual medical severity of the overdose from psychiatric factors resulted in a dangerous underestimation of the risk of patients with Briquet's syndrome and an overprediction in the case of primary affective disorder.", "contents": "Psychiatric diagnosis and tricyclic plasma levels in 36 hospitalized overdose patients. Thirty-six hospitalized patients who were considered by emergency room physicians to have ingested medically serious tricyclic overdoses were studied. The purpose of the project was to determine if psychiatric diagnosis and associated psychiatric factors correlated with the severity of the overdose as defined by plasma drug levels. Medically serious overdoses were ingested by patients with alcoholism, primary affective disorder, undiagnosed psychiatric illness, and Briquet's syndrome. Prior psychiatric treatment, prior admissions, prior overdoses, or precipitating events did not correlate with the medical severity of the ingestion. Attempting to predict the individual medical severity of the overdose from psychiatric factors resulted in a dangerous underestimation of the risk of patients with Briquet's syndrome and an overprediction in the case of primary affective disorder.", "PMID": 972331}
{"id": "pubmed23n0032_8965", "title": "Microsurgery of Arnold-Chiari malformation in adults with and without hydromyelia.", "content": "Microsurgical exploration of 15 adults with Arnold-Chiari malformation with and without hydromyelia using 3 to 20 X magnification has led to the following conclusions. Hydromyelia, associated with Arnold-Chiari malformation, is a progressive mechanical disorder that causes spinal cord deficits by pressure distention of the cord. Arnold-Chiari malformation causes slowly or suddenly progressive bulbar dysfunction by impaction of the malformation in the foramen magnum. Decompression of both can be achieved by a suboccipital carniectomy, upper cervical laminectomy, establishing an outlet from the fourth ventricle, and opening the distended cord in the thinnest exposed area, which is usually along the dorsal root entry zone. If Pantopaque myelography in patients in the supine position shows the Arnold-Chiari malformation, hydromyelia can be established as a cause of central cord deficit even if myelography shows the cord size to be normal. Syringomyelia, traditionally considered a degenerative disease, is a less common cause of a slowly progressive central cord deficit than either hydromyelia or intramedullary tumor.", "contents": "Microsurgery of Arnold-Chiari malformation in adults with and without hydromyelia. Microsurgical exploration of 15 adults with Arnold-Chiari malformation with and without hydromyelia using 3 to 20 X magnification has led to the following conclusions. Hydromyelia, associated with Arnold-Chiari malformation, is a progressive mechanical disorder that causes spinal cord deficits by pressure distention of the cord. Arnold-Chiari malformation causes slowly or suddenly progressive bulbar dysfunction by impaction of the malformation in the foramen magnum. Decompression of both can be achieved by a suboccipital carniectomy, upper cervical laminectomy, establishing an outlet from the fourth ventricle, and opening the distended cord in the thinnest exposed area, which is usually along the dorsal root entry zone. If Pantopaque myelography in patients in the supine position shows the Arnold-Chiari malformation, hydromyelia can be established as a cause of central cord deficit even if myelography shows the cord size to be normal. Syringomyelia, traditionally considered a degenerative disease, is a less common cause of a slowly progressive central cord deficit than either hydromyelia or intramedullary tumor.", "PMID": 972332}
{"id": "pubmed23n0032_8966", "title": "Proximal occlusion of the dominant anterior cerebral artery for anterior communicating aneurysm.", "content": "Twenty-seven patients with ruptured and one with symptomatic unruptured anterior communicating aneurysms were treated by proximal clip occlusion of the dominant anterior cerebral artery. These patients have been followed for 2 to 4 1/2 years and the results evaluated. The operation carried a very low mortality rate (3.5%) and a relatively low morbidity. There was no instance of recurrent hemorrhage during the study period. Clinical and preoperative angiographic criteria for selection of patients and the timing and technique of surgery are discussed.", "contents": "Proximal occlusion of the dominant anterior cerebral artery for anterior communicating aneurysm. Twenty-seven patients with ruptured and one with symptomatic unruptured anterior communicating aneurysms were treated by proximal clip occlusion of the dominant anterior cerebral artery. These patients have been followed for 2 to 4 1/2 years and the results evaluated. The operation carried a very low mortality rate (3.5%) and a relatively low morbidity. There was no instance of recurrent hemorrhage during the study period. Clinical and preoperative angiographic criteria for selection of patients and the timing and technique of surgery are discussed.", "PMID": 972333}
{"id": "pubmed23n0032_8967", "title": "Microvascular bypass surgery. Part 2: physiological studies.", "content": "Intraoperative electromagnetic blood flow measurements were recorded in 15 patients undergoing extracranial-intracranial arterial bypass surgery. The initial average blood flow of 28.2 cc/min supplies 8.4% of the expected flow from one internal carotid artery. Blood flow probably increases postoperatively, as evidenced by angiographic enlargement of the bypass and its recipient cortical arteries.", "contents": "Microvascular bypass surgery. Part 2: physiological studies. Intraoperative electromagnetic blood flow measurements were recorded in 15 patients undergoing extracranial-intracranial arterial bypass surgery. The initial average blood flow of 28.2 cc/min supplies 8.4% of the expected flow from one internal carotid artery. Blood flow probably increases postoperatively, as evidenced by angiographic enlargement of the bypass and its recipient cortical arteries.", "PMID": 972334}
{"id": "pubmed23n0032_8968", "title": "Endothelial cell damage by temporary arterial occlusion with surgical clips. Study of the clip site by scanning and transmission electron microscopy.", "content": "The effects of temporary vascular occlusion with surgical clips on the underlying endothelial lining were studied with scanning (SEM) and transmission (TEM) electron microscopy. Twenty-five rabbits were anesthetized and both common carotid arteries exposed. A Heifetz clip was used to occlude the right carotid artery for 5, 15, and 30 minutes, and 2 hours in five animals each. The clips were removed and the vessels immediately perfused with glutaraldehyde. In five remaining animals, the right carotid arteries were occluded for 30 minutes followed by removal of the clip and resumption of blood flow for 30 minutes prior to fixation. Combined SEM and TEM examination of the endothelium of compressed segments revealed \"craters\" and \"balloons\", blebs and vacuoles, swollen mitochondria, dilated granular endoplasmic reticulum, and subendothelial edema. There were also areas of endothelial cell flattening, discontinuity, and desquamation exposing the subendothelial tissues. Following restoration of flow, platelets and fibrin were found adherent to altered endothelial cells and to exposed subendothelial tissues. Endothelial craters and balloons were also found distal and, significantly less frequently, proximal to the site of occlusion. It is suggested that antiplatelet aggregating agents may prove beneficial for the prevention of thrombus formation at the site of the clip as well as craters and balloons distal to the clip following procedures requiring temporary vascular occlusion.", "contents": "Endothelial cell damage by temporary arterial occlusion with surgical clips. Study of the clip site by scanning and transmission electron microscopy. The effects of temporary vascular occlusion with surgical clips on the underlying endothelial lining were studied with scanning (SEM) and transmission (TEM) electron microscopy. Twenty-five rabbits were anesthetized and both common carotid arteries exposed. A Heifetz clip was used to occlude the right carotid artery for 5, 15, and 30 minutes, and 2 hours in five animals each. The clips were removed and the vessels immediately perfused with glutaraldehyde. In five remaining animals, the right carotid arteries were occluded for 30 minutes followed by removal of the clip and resumption of blood flow for 30 minutes prior to fixation. Combined SEM and TEM examination of the endothelium of compressed segments revealed \"craters\" and \"balloons\", blebs and vacuoles, swollen mitochondria, dilated granular endoplasmic reticulum, and subendothelial edema. There were also areas of endothelial cell flattening, discontinuity, and desquamation exposing the subendothelial tissues. Following restoration of flow, platelets and fibrin were found adherent to altered endothelial cells and to exposed subendothelial tissues. Endothelial craters and balloons were also found distal and, significantly less frequently, proximal to the site of occlusion. It is suggested that antiplatelet aggregating agents may prove beneficial for the prevention of thrombus formation at the site of the clip as well as craters and balloons distal to the clip following procedures requiring temporary vascular occlusion.", "PMID": 972335}
{"id": "pubmed23n0032_8969", "title": "Fluid-percussion model of mechanical brain injury in the cat.", "content": "Mechanical brain injury was produced in 36 cats with a fluid-percussion model in which brain damage or dysfunction is produced by a single, brief, hydraulically-induced pressure transient that is conducted through the brain. Fluid-percussion injury induce elastic deformation of the brain resembling the brain deformation known to occur following head impact. Physiological responses and pahtological changes following injury were expressed as a function of peak pressure. Macroscopic central nervous system lesions concentrated at the pontomesencephalic junction, cervicomedullary junction, and in the cerebellar tonsils were consistently observed at and above 2.6 atmospheres (atm). At higher levels of injury (greater than or equal to 3.2 atm) there was extensive basal subarachnoid hemorrhage. At very high levels of injury (greater than 4.0 atm) hemorrhagic contusions were noted at the cerebral hemisphere impact site. A spectrum of neuronal alterations was identified in the damaged areas. Computer analysis showed correlation of electroencephalographic (EEG) changes with the neuropathological changes, since EEG recovery became severely impaired above 2.6 atm. No EEG changes were noted below 1.5 atm. From 1.5 to 2.2 atm there was a physiological response to injury but no significant changes were seen on neuropathological examination. This range of injury should permit further studies of the more subtle changes following mechanical brain injury without intraparenchymal hemorrhage or subarachnoid hemorrhage. The fluid-percussion model relates brain deformation following mechanical loading to a single pressure transient that is easily measured and controlled. Further quantitative investigation into the pathobiology of mechanical brain injury following graded brain deformation is thus made possible.", "contents": "Fluid-percussion model of mechanical brain injury in the cat. Mechanical brain injury was produced in 36 cats with a fluid-percussion model in which brain damage or dysfunction is produced by a single, brief, hydraulically-induced pressure transient that is conducted through the brain. Fluid-percussion injury induce elastic deformation of the brain resembling the brain deformation known to occur following head impact. Physiological responses and pahtological changes following injury were expressed as a function of peak pressure. Macroscopic central nervous system lesions concentrated at the pontomesencephalic junction, cervicomedullary junction, and in the cerebellar tonsils were consistently observed at and above 2.6 atmospheres (atm). At higher levels of injury (greater than or equal to 3.2 atm) there was extensive basal subarachnoid hemorrhage. At very high levels of injury (greater than 4.0 atm) hemorrhagic contusions were noted at the cerebral hemisphere impact site. A spectrum of neuronal alterations was identified in the damaged areas. Computer analysis showed correlation of electroencephalographic (EEG) changes with the neuropathological changes, since EEG recovery became severely impaired above 2.6 atm. No EEG changes were noted below 1.5 atm. From 1.5 to 2.2 atm there was a physiological response to injury but no significant changes were seen on neuropathological examination. This range of injury should permit further studies of the more subtle changes following mechanical brain injury without intraparenchymal hemorrhage or subarachnoid hemorrhage. The fluid-percussion model relates brain deformation following mechanical loading to a single pressure transient that is easily measured and controlled. Further quantitative investigation into the pathobiology of mechanical brain injury following graded brain deformation is thus made possible.", "PMID": 972336}
{"id": "pubmed23n0032_8970", "title": "Effect of increasing the plasma oxygen diffusivity on experimental cryogenic edema.", "content": "Mongrel cats with experimental cryogenic brain lesions were treated with the carotenoid compound crocetin. It has been shown that crocetin increases the diffusion speed of oxygen through plasma, and should provide a net increase in available oxygen to the capillary endothelial cell. The treated group of animals showed a significant reduction in edema as compared to a comparable control group. It is suggested that oxygen availability is an important factor in vasogenic edema.", "contents": "Effect of increasing the plasma oxygen diffusivity on experimental cryogenic edema. Mongrel cats with experimental cryogenic brain lesions were treated with the carotenoid compound crocetin. It has been shown that crocetin increases the diffusion speed of oxygen through plasma, and should provide a net increase in available oxygen to the capillary endothelial cell. The treated group of animals showed a significant reduction in edema as compared to a comparable control group. It is suggested that oxygen availability is an important factor in vasogenic edema.", "PMID": 972337}
{"id": "pubmed23n0032_8971", "title": "Activation of neurons in the cerebellar nuclei and ascending reticular formation by stimulation of the cerebellar surface.", "content": "Electrical stimulation of the cerebellar surface has been used therapeutically for the control of certain epileptic seizure and motor disorders. Recent hypotheses suggest that the therapeutic results in the treatment of epilepsy might be a consequence of the activation of Purkinje cells which subsequently inhibit the epileptic activity in the cerebrocellular loop. These experiments establish that an anatomical substrate exists whereby the effects of stimulating the cerebellar surface might be mediated by the ascending reticular formation and the non-specific thalamic nuclei. Specifically, the stimulation of the cerebellar surface activates not only Purkinje cells but also cerebellar afferent systems, climbing fibers and mossy fibers, and neurons in the cerebellar nuclei and reticular formation. In addition, recordings from neurons in the ascending reticular formation suggest that stimulation of the cerebellar surface can affect processing of ascending sensory information, thus influencing neural integration of non-specific sensory system.", "contents": "Activation of neurons in the cerebellar nuclei and ascending reticular formation by stimulation of the cerebellar surface. Electrical stimulation of the cerebellar surface has been used therapeutically for the control of certain epileptic seizure and motor disorders. Recent hypotheses suggest that the therapeutic results in the treatment of epilepsy might be a consequence of the activation of Purkinje cells which subsequently inhibit the epileptic activity in the cerebrocellular loop. These experiments establish that an anatomical substrate exists whereby the effects of stimulating the cerebellar surface might be mediated by the ascending reticular formation and the non-specific thalamic nuclei. Specifically, the stimulation of the cerebellar surface activates not only Purkinje cells but also cerebellar afferent systems, climbing fibers and mossy fibers, and neurons in the cerebellar nuclei and reticular formation. In addition, recordings from neurons in the ascending reticular formation suggest that stimulation of the cerebellar surface can affect processing of ascending sensory information, thus influencing neural integration of non-specific sensory system.", "PMID": 972338}
{"id": "pubmed23n0032_8972", "title": "Laminotomy and total reconstruction of the posterior spinal arch for spinal canal surgery in childhood.", "content": "Kyphosis, anterior subluxation, and instability of the spine are reported as postoperative complications of multiple level laminectomies in children. The surgical procedure of multiple level laminotomies is proposed as an alternative. Indications in infants and children, the surgical technique, and the postoperative management are presented. The goal is preservation of the normal architecture of the spine in patients who are still developing.", "contents": "Laminotomy and total reconstruction of the posterior spinal arch for spinal canal surgery in childhood. Kyphosis, anterior subluxation, and instability of the spine are reported as postoperative complications of multiple level laminectomies in children. The surgical procedure of multiple level laminotomies is proposed as an alternative. Indications in infants and children, the surgical technique, and the postoperative management are presented. The goal is preservation of the normal architecture of the spine in patients who are still developing.", "PMID": 972339}
{"id": "pubmed23n0032_8973", "title": "Detection of small trigeminal neurinomas.", "content": "Four cases of trigeminal neurinoma are reviewed with particular attention to clinical signs and symptoms, lumbar puncture, electroencephalogram, brain scan, plain skull and tomographic radiographs, and angiographic and pneumoencephalographic findings. Pneumoencephalography, with special tomographic projections to identify various portions of the trigeminal nerve, delineated the tumor in all cases. Tumor removal was complete in three patients and nearly complete in the fourth. There was no operative morbidity or mortality.", "contents": "Detection of small trigeminal neurinomas. Four cases of trigeminal neurinoma are reviewed with particular attention to clinical signs and symptoms, lumbar puncture, electroencephalogram, brain scan, plain skull and tomographic radiographs, and angiographic and pneumoencephalographic findings. Pneumoencephalography, with special tomographic projections to identify various portions of the trigeminal nerve, delineated the tumor in all cases. Tumor removal was complete in three patients and nearly complete in the fourth. There was no operative morbidity or mortality.", "PMID": 972340}
{"id": "pubmed23n0032_8974", "title": "Combined isotope ventriculography and lumbar myelocisternography in the diagnosis of communicating syringomyelia. Case report.", "content": "Several investigative procedures are currently used to diagnose communicating syringomyelia. The combination of isotope ventriculography and lumbar myelocisternography has never proposed nor carried out to verify this condition. We feel that this is a safe and valuable procedure to identify a patent central canal communicating with the fourth ventricle and is worth considering when diagnosing communicating syringomyelia.", "contents": "Combined isotope ventriculography and lumbar myelocisternography in the diagnosis of communicating syringomyelia. Case report. Several investigative procedures are currently used to diagnose communicating syringomyelia. The combination of isotope ventriculography and lumbar myelocisternography has never proposed nor carried out to verify this condition. We feel that this is a safe and valuable procedure to identify a patent central canal communicating with the fourth ventricle and is worth considering when diagnosing communicating syringomyelia.", "PMID": 972341}
{"id": "pubmed23n0032_8975", "title": "Late pseudotumoral brain necrosis following irradiation of a scalp neoplasm. Case report.", "content": "The authors report the case of a 70-year-old man who developed intracranial hypertension and lef-arm paresis 2 years after irradiation for the right preauricular basal cell epithelioma. Angiography disclosed a right temporal lobe mass and the histopathological diagnosis was late postirradiation necrosis of the brain. Postoperatively, dexamethasone was given in increasing doses up to 60 mg/day for control of cerebral edema. The patient died of gastrointestinal bleeding on the 60th postoperative day.", "contents": "Late pseudotumoral brain necrosis following irradiation of a scalp neoplasm. Case report. The authors report the case of a 70-year-old man who developed intracranial hypertension and lef-arm paresis 2 years after irradiation for the right preauricular basal cell epithelioma. Angiography disclosed a right temporal lobe mass and the histopathological diagnosis was late postirradiation necrosis of the brain. Postoperatively, dexamethasone was given in increasing doses up to 60 mg/day for control of cerebral edema. The patient died of gastrointestinal bleeding on the 60th postoperative day.", "PMID": 972342}
{"id": "pubmed23n0032_8976", "title": "Symptomatic Rathke's cleft cyst. Case report.", "content": "A case of a symptomatic Rathke's cleft cyst in a 10-year-old boy is described. The importance of the intraoperative differentiation between this rare tumor and a craniopharyngioma is discussed.", "contents": "Symptomatic Rathke's cleft cyst. Case report. A case of a symptomatic Rathke's cleft cyst in a 10-year-old boy is described. The importance of the intraoperative differentiation between this rare tumor and a craniopharyngioma is discussed.", "PMID": 972343}
{"id": "pubmed23n0032_8977", "title": "Replacement of a self-adjusting bone flap. Technical note.", "content": "Postoperative cerebral edema is often worrisome after intracranial surgery. A self-adjusting bone flap is described that uses sections of Raimondi catheter tubing.", "contents": "Replacement of a self-adjusting bone flap. Technical note. Postoperative cerebral edema is often worrisome after intracranial surgery. A self-adjusting bone flap is described that uses sections of Raimondi catheter tubing.", "PMID": 972344}
{"id": "pubmed23n0032_8978", "title": "Isospora vulpina Nieschulz and Bos, 1933: description, and transmission from the fox (Vulpes vulpes) to the dog.", "content": "Oocysts of Isospora vulpina were found in silver foxes (Vulpes vulpes) on a fox farm in Wisconsin. They were 29.7 (25-38) X 24.3 (21-32) mum. The sporocysts were 17.7 (15-23) X 13 (11-16) mum. Five coccidia-free puppies were inoculated with 22,000-42,000 oocysts each of I. vulpina from the fox: a patent infection resulted after 6-7 days. The infection was then transferred from 1 of these dogs to another coccidia-free puppy. After a 7-day prepatent period the puppy passed oocysts for 7 days.", "contents": "Isospora vulpina Nieschulz and Bos, 1933: description, and transmission from the fox (Vulpes vulpes) to the dog. Oocysts of Isospora vulpina were found in silver foxes (Vulpes vulpes) on a fox farm in Wisconsin. They were 29.7 (25-38) X 24.3 (21-32) mum. The sporocysts were 17.7 (15-23) X 13 (11-16) mum. Five coccidia-free puppies were inoculated with 22,000-42,000 oocysts each of I. vulpina from the fox: a patent infection resulted after 6-7 days. The infection was then transferred from 1 of these dogs to another coccidia-free puppy. After a 7-day prepatent period the puppy passed oocysts for 7 days.", "PMID": 972349}
{"id": "pubmed23n0032_8979", "title": "Fine structure of an unidentified protozoon in the epithelium of rainbow trout exposed to water with Myxosoma cerebralis.", "content": "An intracellular protozoon was discovered in the epithelium of young rainbow trout (Salmo gairdneri) exposed for as short a time as 1 hr to water known to contain infective stages of Myxosoma cerebralis. Light- and electron-microscopic examination of this tissue revealed what appeared to be a proliferative stage (presumptive schizont) of a sporozoon; other possible stages in the life cycle were also observed. The relationship of this unidentified protozoon of M. cerebralis remains unresolved.", "contents": "Fine structure of an unidentified protozoon in the epithelium of rainbow trout exposed to water with Myxosoma cerebralis. An intracellular protozoon was discovered in the epithelium of young rainbow trout (Salmo gairdneri) exposed for as short a time as 1 hr to water known to contain infective stages of Myxosoma cerebralis. Light- and electron-microscopic examination of this tissue revealed what appeared to be a proliferative stage (presumptive schizont) of a sporozoon; other possible stages in the life cycle were also observed. The relationship of this unidentified protozoon of M. cerebralis remains unresolved.", "PMID": 972350}
{"id": "pubmed23n0032_8980", "title": "Myxosoma cerebralis: in vitro sporulation of the myxosporidan of salmonid whirling disease.", "content": "Trophozoites and other pre-spore stages of the myxosporidan Myxosoma cerebralis were taken from infected rainbow trout (Salmo gairdneri) and cultured in vitro. Cultures eventually yielded mature spores capable of discharging their polar capsules. This is the first report of culture of a myxosporidan.", "contents": "Myxosoma cerebralis: in vitro sporulation of the myxosporidan of salmonid whirling disease. Trophozoites and other pre-spore stages of the myxosporidan Myxosoma cerebralis were taken from infected rainbow trout (Salmo gairdneri) and cultured in vitro. Cultures eventually yielded mature spores capable of discharging their polar capsules. This is the first report of culture of a myxosporidan.", "PMID": 972351}
{"id": "pubmed23n0032_8981", "title": "Immunologic and electrophoretic characteristics of two species of Crithidia.", "content": "Crithidia harmosa and Crithidia fasciculata were compared immunologically by the indirect fluorescent antibody (FA) method and by agglutination. The FA technic yielded more specific results with cellular-debris than with whole-cell antigens. The immune sera collected from chickens 9 days after the last inoculations with whole-cell antigens had higher homologous titers than those collected after 4 days. Major antigenic differences between the 2 species were revealed by both methods. The electrophoretic patterns of C. harmosa and C. fasciculata obtained by polyacrylamide gel slab electrophoresis differed in the numbers and relative mobilities of their component bands.", "contents": "Immunologic and electrophoretic characteristics of two species of Crithidia. Crithidia harmosa and Crithidia fasciculata were compared immunologically by the indirect fluorescent antibody (FA) method and by agglutination. The FA technic yielded more specific results with cellular-debris than with whole-cell antigens. The immune sera collected from chickens 9 days after the last inoculations with whole-cell antigens had higher homologous titers than those collected after 4 days. Major antigenic differences between the 2 species were revealed by both methods. The electrophoretic patterns of C. harmosa and C. fasciculata obtained by polyacrylamide gel slab electrophoresis differed in the numbers and relative mobilities of their component bands.", "PMID": 972352}
{"id": "pubmed23n0032_8982", "title": "Specificity of the glucose transport system in Leishmania tropica promastigotes.", "content": "The glucose transport system in Leishmania tropica promastigotes was characterized by the use of labeled 2-deoxy-D-glucose (2-DOG), a nonmetabolizable glucose analog. The uptake system has a Q10 of 2 and a heat of activation of 10.2 kcal/mole. The glucose transport system is subject to competitive inhibition by 2-DOG, glucosamine, N-acetyl glucosamine, mannose, galactose, and fructose which suggests that substitutions in the hexose chain at carbons 2 and 4 do not affect carrier specificity. In contrast, changes at carbon 1 (alpha-methyl-D-glucoside, 1,5-anhydroglucitol) and carbon 3 (3-0-methyl glucose) lead to loss of carrier affinity since these sugars do not compete for the glucose carrier. Sugars that compete with the glucose carrier have one common feature--they all exist in the pyranose form in solution. The carrier for D-glucose does not interact with L-glucose or any of the pentose sugars tested. Uptake of 2-DOG is inhibited by glycerol. This inhibition, however, is noncompetitive; it is evident; therefore, that glucose and glycerol do not compete for the same carrier. Glycerol does not repress the glucose carrier since cells grown in presence of glycerol transport the sugar normally.", "contents": "Specificity of the glucose transport system in Leishmania tropica promastigotes. The glucose transport system in Leishmania tropica promastigotes was characterized by the use of labeled 2-deoxy-D-glucose (2-DOG), a nonmetabolizable glucose analog. The uptake system has a Q10 of 2 and a heat of activation of 10.2 kcal/mole. The glucose transport system is subject to competitive inhibition by 2-DOG, glucosamine, N-acetyl glucosamine, mannose, galactose, and fructose which suggests that substitutions in the hexose chain at carbons 2 and 4 do not affect carrier specificity. In contrast, changes at carbon 1 (alpha-methyl-D-glucoside, 1,5-anhydroglucitol) and carbon 3 (3-0-methyl glucose) lead to loss of carrier affinity since these sugars do not compete for the glucose carrier. Sugars that compete with the glucose carrier have one common feature--they all exist in the pyranose form in solution. The carrier for D-glucose does not interact with L-glucose or any of the pentose sugars tested. Uptake of 2-DOG is inhibited by glycerol. This inhibition, however, is noncompetitive; it is evident; therefore, that glucose and glycerol do not compete for the same carrier. Glycerol does not repress the glucose carrier since cells grown in presence of glycerol transport the sugar normally.", "PMID": 972353}
{"id": "pubmed23n0032_8983", "title": "Activities of glucose-6-phosphate, 6-phosphogluconate, and isocitrate dehydrogenases from Leishmania donovani cultivated at 25 and 37 C.", "content": "The activities of glucose-6-phosphate dehydrogenase (G-6-PD) (EC No. 1.1.1.49), 6-phosphogluconate dehydrogenase (PGD) (EC No. 1.1.1.44), and isocitrate dehydrogenase (ICD) (EC No. 1.1.1.42) from promastigotes of Leishmania donovani strain 3S grown at 25 C in modified Tobie's (mT) medium and from promastigotes of the 37 C-adapted substrain of this strain cultivated in the mT at 37 C were assayed at 25 and 37 C. At 25 C ICD from both the strain and the substrain had the highest, and PGD, the lowest activity; the activity of G-6-PD was intermediate, but much closer to that of ICD. Irrespective of the temperature of the assay, the activities of G-6-PD and ICD from the 37 C substrain were significantly higher than those of these enzymes from the parental strain; however, the activity of PGD from the 25 C strain was slightly higher than that of this dehydrogenase from the 37 C-adapted stock. No significant activity losses of G-6-PD and ICD from either the strain or the substrain were noted after incubation of the extracts in the presence of 0.25 M sucrose at 37 C for 2 hr. PGD was unstable in such extracts, but it could be rendered stable by the addition of 4 mM 6-phosphogluconate, G-6-PD was the least and ICD the most dependent on Mg2+ ions. In the 15-25 C range, the Q10 values of the enzymes from the 25 C strain were 2.83, 2.5 and 2.63 for G-6-PD, PGD, and ICD, respectively. These values for the respective enzymes in the 25-35 C range were 2.06, 1.67, and 1.62. The Q10 values of the enzymes from the 37 C substrain in the 15-25 C range were 2.06 for G-6-PD, 3.25 for PGD, and 2.77 for ICD; in the 25-35 C range, the corresponding values were 1.67, 1.46, and 1.83. Cultivation of the 37 C substrain at 25 C was accompanied by a drop in G-6-PD and ICD activities.", "contents": "Activities of glucose-6-phosphate, 6-phosphogluconate, and isocitrate dehydrogenases from Leishmania donovani cultivated at 25 and 37 C. The activities of glucose-6-phosphate dehydrogenase (G-6-PD) (EC No. 1.1.1.49), 6-phosphogluconate dehydrogenase (PGD) (EC No. 1.1.1.44), and isocitrate dehydrogenase (ICD) (EC No. 1.1.1.42) from promastigotes of Leishmania donovani strain 3S grown at 25 C in modified Tobie's (mT) medium and from promastigotes of the 37 C-adapted substrain of this strain cultivated in the mT at 37 C were assayed at 25 and 37 C. At 25 C ICD from both the strain and the substrain had the highest, and PGD, the lowest activity; the activity of G-6-PD was intermediate, but much closer to that of ICD. Irrespective of the temperature of the assay, the activities of G-6-PD and ICD from the 37 C substrain were significantly higher than those of these enzymes from the parental strain; however, the activity of PGD from the 25 C strain was slightly higher than that of this dehydrogenase from the 37 C-adapted stock. No significant activity losses of G-6-PD and ICD from either the strain or the substrain were noted after incubation of the extracts in the presence of 0.25 M sucrose at 37 C for 2 hr. PGD was unstable in such extracts, but it could be rendered stable by the addition of 4 mM 6-phosphogluconate, G-6-PD was the least and ICD the most dependent on Mg2+ ions. In the 15-25 C range, the Q10 values of the enzymes from the 25 C strain were 2.83, 2.5 and 2.63 for G-6-PD, PGD, and ICD, respectively. These values for the respective enzymes in the 25-35 C range were 2.06, 1.67, and 1.62. The Q10 values of the enzymes from the 37 C substrain in the 15-25 C range were 2.06 for G-6-PD, 3.25 for PGD, and 2.77 for ICD; in the 25-35 C range, the corresponding values were 1.67, 1.46, and 1.83. Cultivation of the 37 C substrain at 25 C was accompanied by a drop in G-6-PD and ICD activities.", "PMID": 972354}
{"id": "pubmed23n0032_8984", "title": "Effect of glucose on glycine requirement of Acanthamoeba castellanii.", "content": "Acanthamoeba castellanii grows in a minimal medium (AMLIV) containing only arginine, methionine, leucine, isoleucine, and valine as sole nitrogen sources, other than vitamins, when glucose is the carbon source. With acetate as the carbon source, glycine must be added to AMLIV. Doubling time in AMLIV varies according to the ratio of amino acids concentrations. Several combinations yield Td values of approximately 70 hr.", "contents": "Effect of glucose on glycine requirement of Acanthamoeba castellanii. Acanthamoeba castellanii grows in a minimal medium (AMLIV) containing only arginine, methionine, leucine, isoleucine, and valine as sole nitrogen sources, other than vitamins, when glucose is the carbon source. With acetate as the carbon source, glycine must be added to AMLIV. Doubling time in AMLIV varies according to the ratio of amino acids concentrations. Several combinations yield Td values of approximately 70 hr.", "PMID": 972355}
{"id": "pubmed23n0032_8985", "title": "The socialization of a Medical School faculty.", "content": "The research reported here shows the processes which socialize M.D.s and Ph.D.s into academic roles in a medical school and the factors affecting their retention and attrition. Eighty-one percent (350) of the regular faculty of a midwestern university medical school provided career background data and information on their attitudes toward issues within their school and their perceived and preferred organizational goals, characteristics, and managerial styles. Sherlock and Morris' (1) paradigm was the theoretical framework. Principal findings include the greater importance of latent over manifest variables, the M.D.s' late decision to try an academic career, the high colleague acceptance of the new M.D. recruit, the greater adoption of university professoriat norms by the Ph.D.s, and the high goal agreement between basic science and clinical faculty.", "contents": "The socialization of a Medical School faculty. The research reported here shows the processes which socialize M.D.s and Ph.D.s into academic roles in a medical school and the factors affecting their retention and attrition. Eighty-one percent (350) of the regular faculty of a midwestern university medical school provided career background data and information on their attitudes toward issues within their school and their perceived and preferred organizational goals, characteristics, and managerial styles. Sherlock and Morris' (1) paradigm was the theoretical framework. Principal findings include the greater importance of latent over manifest variables, the M.D.s' late decision to try an academic career, the high colleague acceptance of the new M.D. recruit, the greater adoption of university professoriat norms by the Ph.D.s, and the high goal agreement between basic science and clinical faculty.", "PMID": 972370}
{"id": "pubmed23n0032_8986", "title": "An NIH/Columbia University information system interface.", "content": "The National Institutes of Health (NIH) and the Health Sciences of Columbia University have developed an information system interface whereby sponsored research information is routinely forwarded, in machine-readable form, from the NIH to Columbia University. For Columbia University the interface system has materially reduced data input costs, made information available on a more timely basis, improved the quality of its system by the adoption of existing standardized data definitions, and allowed flexible access to NIH's broad data base. For NIH the system permits an improvement in its data base through high-quality and fast feedback from the grantee institution. The promotion of future systems development is enhanced through the adoption of standardized data element designations and definitions.", "contents": "An NIH/Columbia University information system interface. The National Institutes of Health (NIH) and the Health Sciences of Columbia University have developed an information system interface whereby sponsored research information is routinely forwarded, in machine-readable form, from the NIH to Columbia University. For Columbia University the interface system has materially reduced data input costs, made information available on a more timely basis, improved the quality of its system by the adoption of existing standardized data definitions, and allowed flexible access to NIH's broad data base. For NIH the system permits an improvement in its data base through high-quality and fast feedback from the grantee institution. The promotion of future systems development is enhanced through the adoption of standardized data element designations and definitions.", "PMID": 972371}
{"id": "pubmed23n0032_8987", "title": "A method for defining competency in Pediatrics.", "content": "In 1972 the American Board of Pediatrics (ABP) initiated studies leading to a report which identifies the important components of competency needed in the practice of pediatrics. The development of the report involved a group process engaging ABP board members and examiners. The outcome has been (a) the delineation of a process for establishing definitions of competency in a medical discipline; (b) the development of a framework for specifying the abilities needed to perform the tasks required of pediatricians; (c) an elaboration of samples of pediatric subjects to which these abilities and tasks are relevant; and (d) the development of a basis for choice of methods to use for purposes of certification.", "contents": "A method for defining competency in Pediatrics. In 1972 the American Board of Pediatrics (ABP) initiated studies leading to a report which identifies the important components of competency needed in the practice of pediatrics. The development of the report involved a group process engaging ABP board members and examiners. The outcome has been (a) the delineation of a process for establishing definitions of competency in a medical discipline; (b) the development of a framework for specifying the abilities needed to perform the tasks required of pediatricians; (c) an elaboration of samples of pediatric subjects to which these abilities and tasks are relevant; and (d) the development of a basis for choice of methods to use for purposes of certification.", "PMID": 972372}
{"id": "pubmed23n0032_8988", "title": "A study of the demand for part-time residency programs.", "content": "A survey was conducted at the Medical College of Pennsylvania to measure the magnitude of demand for part-time residency programs and to assess the motivations underlying such interest. Responses from 206 females and 96 males (84 percent of all students) indicated that approximately 39 percent would probably or definitely apply to part-time residencies if they were available. As was expected, interest was greater among women and was greatest among women who were parents. At the same time, however, there was considerable interest which was not related to marital or parental status and which derived from a general dissatisfaction with the time demands of traditional graduate medical training. While there are many other factors to be considered, the demand for part-time residency programs probably far exceeds the supply of such programs and, with the continued expansion of the numbers of women physicians, will probably continue to grow.", "contents": "A study of the demand for part-time residency programs. A survey was conducted at the Medical College of Pennsylvania to measure the magnitude of demand for part-time residency programs and to assess the motivations underlying such interest. Responses from 206 females and 96 males (84 percent of all students) indicated that approximately 39 percent would probably or definitely apply to part-time residencies if they were available. As was expected, interest was greater among women and was greatest among women who were parents. At the same time, however, there was considerable interest which was not related to marital or parental status and which derived from a general dissatisfaction with the time demands of traditional graduate medical training. While there are many other factors to be considered, the demand for part-time residency programs probably far exceeds the supply of such programs and, with the continued expansion of the numbers of women physicians, will probably continue to grow.", "PMID": 972373}
{"id": "pubmed23n0032_8989", "title": "A formative and summative evaluation model for special educational programs.", "content": "During the past two decades the problems of recruiting minority students into careers in the health fields have been addressed by many educators and various special study groups. Recent efforts by schools of medicine and allied health sciences to find viable solutions to the problem have facilitated implementation of career exploration and study-skills programs. Although the focus and sophistication of evaluation designs vary substantially among educational programs of this type, all share some common problems. The positions taken by the authors is that evaluation, both during and at the end of a program, is an essential aspect of the program and that the use of a systematic evaluation model is central to this effort. Data are presented which illustrate the utility of a systematic evaluation model when applied in a realistic test situation (an operational program for minority students). Several advantages of using this model are discussed.", "contents": "A formative and summative evaluation model for special educational programs. During the past two decades the problems of recruiting minority students into careers in the health fields have been addressed by many educators and various special study groups. Recent efforts by schools of medicine and allied health sciences to find viable solutions to the problem have facilitated implementation of career exploration and study-skills programs. Although the focus and sophistication of evaluation designs vary substantially among educational programs of this type, all share some common problems. The positions taken by the authors is that evaluation, both during and at the end of a program, is an essential aspect of the program and that the use of a systematic evaluation model is central to this effort. Data are presented which illustrate the utility of a systematic evaluation model when applied in a realistic test situation (an operational program for minority students). Several advantages of using this model are discussed.", "PMID": 972374}
{"id": "pubmed23n0032_8990", "title": "Designing a curriculum in a clinical setting: an iterative process.", "content": "A curriculum for the training of medical students was designed and implemented in a functioning clinical setting. The multidisciplinary, multiprofessional staff of a primary care center participated with professional educators in an iterative process for curriculum development. A three-stage plan was conceived: behaviorally oriented educational objectives were constructed, instructional methodologies to satisfy these objectives were created, and evaluation instruments were designed. Throughout each stage the educators facilitated the process by teaching the staff the necessary techniques for the design and implementation of the curriculum. The curriculum that resulted from this process is focused on those issues that are important to team delivery of primary care. An important outcome of the project is the increased enthusiasm and competence of the professional staff in the teaching of students in the health professions.", "contents": "Designing a curriculum in a clinical setting: an iterative process. A curriculum for the training of medical students was designed and implemented in a functioning clinical setting. The multidisciplinary, multiprofessional staff of a primary care center participated with professional educators in an iterative process for curriculum development. A three-stage plan was conceived: behaviorally oriented educational objectives were constructed, instructional methodologies to satisfy these objectives were created, and evaluation instruments were designed. Throughout each stage the educators facilitated the process by teaching the staff the necessary techniques for the design and implementation of the curriculum. The curriculum that resulted from this process is focused on those issues that are important to team delivery of primary care. An important outcome of the project is the increased enthusiasm and competence of the professional staff in the teaching of students in the health professions.", "PMID": 972375}
{"id": "pubmed23n0032_8991", "title": "[Anti reflux plasty by submucous \"advancement\" of the ureter. An experience of 384 malformative vesico-renal cases which were operated (author's transl)].", "content": "The authors present their experience of 384 ureters which were operated for malformative vesico-renal reflux by the technique of submucous advancement of the ureter. The quality of the results from the standpoints of ureteric function and that of sterility of the urine shows the quality of this technique.", "contents": "[Anti reflux plasty by submucous \"advancement\" of the ureter. An experience of 384 malformative vesico-renal cases which were operated (author's transl)]. The authors present their experience of 384 ureters which were operated for malformative vesico-renal reflux by the technique of submucous advancement of the ureter. The quality of the results from the standpoints of ureteric function and that of sterility of the urine shows the quality of this technique.", "PMID": 972410}
{"id": "pubmed23n0032_8992", "title": "[Stress incontinence: symptoms. Anatomo-radiological and urodynamic exploration by flow-metry (author's transl)].", "content": "The authors study stress incontinence, symptom and not disease, an entity which is rarely found in the pure state in the 21 cases presented. After analysis of the physio-pathological factors concerning stress incontinence, they present the results of a dynamic study by flow measurement (flow-metry). This enabled division of the incontinent patients into 3 groups, depending on the amount of flow, on micturition, normal, increased, decreased. Each group has common anatomo-radiological points, perhaps permitting a new dynamic classification of symptomatic stress incontinence.", "contents": "[Stress incontinence: symptoms. Anatomo-radiological and urodynamic exploration by flow-metry (author's transl)]. The authors study stress incontinence, symptom and not disease, an entity which is rarely found in the pure state in the 21 cases presented. After analysis of the physio-pathological factors concerning stress incontinence, they present the results of a dynamic study by flow measurement (flow-metry). This enabled division of the incontinent patients into 3 groups, depending on the amount of flow, on micturition, normal, increased, decreased. Each group has common anatomo-radiological points, perhaps permitting a new dynamic classification of symptomatic stress incontinence.", "PMID": 972419}
{"id": "pubmed23n0032_8993", "title": "[Socalled \"primary\" megaureter in the child and adult. Comparative study of 46 megaureters (author's transl)].", "content": "This analysis covering 46 MU in 36 patients, of which 16 were adults, shows the greater gravity in the child, with regards to the renal lesions as well as the degree of ureteric dilatation. The advantage of quantitative renal scintigraphic scanning in the evaluation of renal involvement is stressed. On the other hand, with regards to the results of conservative surgery (21 Leedbetter-Politano antireflux uretero-vesical re-implantations of which 5 were complemented by Hendren type modeling ureteroplasty) there seems to be no difference between the children and adults: 18 good results, 2 of which after reintervention for stenosis and uretero-vesical reimplantation.", "contents": "[Socalled \"primary\" megaureter in the child and adult. Comparative study of 46 megaureters (author's transl)]. This analysis covering 46 MU in 36 patients, of which 16 were adults, shows the greater gravity in the child, with regards to the renal lesions as well as the degree of ureteric dilatation. The advantage of quantitative renal scintigraphic scanning in the evaluation of renal involvement is stressed. On the other hand, with regards to the results of conservative surgery (21 Leedbetter-Politano antireflux uretero-vesical re-implantations of which 5 were complemented by Hendren type modeling ureteroplasty) there seems to be no difference between the children and adults: 18 good results, 2 of which after reintervention for stenosis and uretero-vesical reimplantation.", "PMID": 972423}
{"id": "pubmed23n0032_8994", "title": "[Transperineal prostatography for the diagnosis of cancer of the prostate (author's transl)].", "content": "Prostatography by perineal puncture is a remarkable technique for examination of patients with prostatic disorders. The authors prefer transperineal puncture of the prostate for the following reasons: 1) It is carried out under the guidance of the free right index finger in the rectum; 2) Infection has never been observed; 3) Stimultaneous biopsies taken from various regions of the prostate, for histological examination, is possible on changing the position of the needle. Perineal prostatography enabled one to: 1. Precisely see the extent of a cnacer. 2. Discover lesions provoked by the tumor (necrotic zones, opacities). 3. Assert that extracapsular extension has taken place when the contrast medium has diffusep outside of the prostatic capsule. 4. Possibly establish the presence of latent carcinoma within a prostatic adenoma. These being all useful notions for treatment.", "contents": "[Transperineal prostatography for the diagnosis of cancer of the prostate (author's transl)]. Prostatography by perineal puncture is a remarkable technique for examination of patients with prostatic disorders. The authors prefer transperineal puncture of the prostate for the following reasons: 1) It is carried out under the guidance of the free right index finger in the rectum; 2) Infection has never been observed; 3) Stimultaneous biopsies taken from various regions of the prostate, for histological examination, is possible on changing the position of the needle. Perineal prostatography enabled one to: 1. Precisely see the extent of a cnacer. 2. Discover lesions provoked by the tumor (necrotic zones, opacities). 3. Assert that extracapsular extension has taken place when the contrast medium has diffusep outside of the prostatic capsule. 4. Possibly establish the presence of latent carcinoma within a prostatic adenoma. These being all useful notions for treatment.", "PMID": 972424}
{"id": "pubmed23n0032_8995", "title": "Defective interfering passages of Sindbis virus: nature of the defective virion RNA.", "content": "Defective interfering particles of Sindbis virus contain 20S RNA identical to that found in BHK cells co-infected with standard and defective virions. We have characterized these RNAs by their oligonucleotide fingerprints. Most of the oligonucleotides were identical to those found in the mRNA (26S RNA) that codes for the virion structural proteins. Three oligonucleotides found in 20S RNA were absent from the 26S RNA pattern and may represent sequences from the 5' end of the virion RNA. Previous difficulties in describing the nature of the defective virion RNA were due to the aggregated state of the RNA. Nucleocapsids obtained from standard and defective virions were essentially the same size and had about the same density, suggesting that defective particles contain more than a single molecule of 20S RNA.", "contents": "Defective interfering passages of Sindbis virus: nature of the defective virion RNA. Defective interfering particles of Sindbis virus contain 20S RNA identical to that found in BHK cells co-infected with standard and defective virions. We have characterized these RNAs by their oligonucleotide fingerprints. Most of the oligonucleotides were identical to those found in the mRNA (26S RNA) that codes for the virion structural proteins. Three oligonucleotides found in 20S RNA were absent from the 26S RNA pattern and may represent sequences from the 5' end of the virion RNA. Previous difficulties in describing the nature of the defective virion RNA were due to the aggregated state of the RNA. Nucleocapsids obtained from standard and defective virions were essentially the same size and had about the same density, suggesting that defective particles contain more than a single molecule of 20S RNA.", "PMID": 972428}
{"id": "pubmed23n0032_8996", "title": "Genome localization of adeno-associated virus RNA.", "content": "In previous work, linear duplex molecules of adeno-associated virus, type 2 (AAV2), DNA were cleaved with the restriction endonucleases R-EcoRI, R-HindII, and R-HindIII. The physical order of the specific fragments obtained was deduced and oriented with respect to the DNA strand polarity and the direction of transcription. Stable AAV RNA is transcribed only from 70% of the minus DNA strand. We report here RNA-DNA hybridization experiments using these restriction fragments to obtain a more accurate map of the portion of the AAV genome represented in stable RNA. The data obtained with several sets of restriction fragments annealed to either whole-cell RNA or poly(A)-containing RNA were internally consistent. The AAV RNA annealed with a continuous region of the AAV DNA, beginning at 0.18 map units (18%) from the left end of the molecule and ending at 0.88 map units. In addition, the restriction endonuclease BamHI was found to make one specific cleavage in AAV2 DNA at 0.22 map units, which is 0.04 map units (i.e., 160 nucleotides) to the right (\"\"down stream'') of the point corresponding to the 5' end of the viral mRNA.", "contents": "Genome localization of adeno-associated virus RNA. In previous work, linear duplex molecules of adeno-associated virus, type 2 (AAV2), DNA were cleaved with the restriction endonucleases R-EcoRI, R-HindII, and R-HindIII. The physical order of the specific fragments obtained was deduced and oriented with respect to the DNA strand polarity and the direction of transcription. Stable AAV RNA is transcribed only from 70% of the minus DNA strand. We report here RNA-DNA hybridization experiments using these restriction fragments to obtain a more accurate map of the portion of the AAV genome represented in stable RNA. The data obtained with several sets of restriction fragments annealed to either whole-cell RNA or poly(A)-containing RNA were internally consistent. The AAV RNA annealed with a continuous region of the AAV DNA, beginning at 0.18 map units (18%) from the left end of the molecule and ending at 0.88 map units. In addition, the restriction endonuclease BamHI was found to make one specific cleavage in AAV2 DNA at 0.22 map units, which is 0.04 map units (i.e., 160 nucleotides) to the right (\"\"down stream'') of the point corresponding to the 5' end of the viral mRNA.", "PMID": 972429}
{"id": "pubmed23n0032_8997", "title": "Isolation of a transcriptive complex from Newcastle disease virions.", "content": "An active transcriptive complex was isolated from purified virions of Newcastle disease virus. After disruption with Triton X-100 and high salt, soluble and particulate fractions were separated by density gradient centrifugation. The transcriptive complex, recovered at a density of 1.275 g/cm3, appeared as a nucleocapsid structure by electron microscopy. When analyzed by polyacryl-amide gel electrophoresis, the nucleocapsids consisted of the nucleocapsid protein, a minor protein of 53,000 molecular weight, and the large L protein. Nucleocapsids possessed less than 1% of the hemagglutinating and neuraminidase activities originally associated with virions. The active complex synthesized predominantly 11 to 20S RNA in vitro and approximately one-fourth of the RNA molecules contained polyadenylic acid segments. In the presence of S-adenosyl-L-methionine, the RNA molecules were capped and methylated at the 5' termini. The transcriptive complex was also capable of methylating exogenous Escherichia coli RNA in the absence of viral RNA synthesis.", "contents": "Isolation of a transcriptive complex from Newcastle disease virions. An active transcriptive complex was isolated from purified virions of Newcastle disease virus. After disruption with Triton X-100 and high salt, soluble and particulate fractions were separated by density gradient centrifugation. The transcriptive complex, recovered at a density of 1.275 g/cm3, appeared as a nucleocapsid structure by electron microscopy. When analyzed by polyacryl-amide gel electrophoresis, the nucleocapsids consisted of the nucleocapsid protein, a minor protein of 53,000 molecular weight, and the large L protein. Nucleocapsids possessed less than 1% of the hemagglutinating and neuraminidase activities originally associated with virions. The active complex synthesized predominantly 11 to 20S RNA in vitro and approximately one-fourth of the RNA molecules contained polyadenylic acid segments. In the presence of S-adenosyl-L-methionine, the RNA molecules were capped and methylated at the 5' termini. The transcriptive complex was also capable of methylating exogenous Escherichia coli RNA in the absence of viral RNA synthesis.", "PMID": 972430}
{"id": "pubmed23n0032_8998", "title": "Replication of T4 DNA in vitro. II. Assay system for and some properties of gene products required for T4 DNA replication.", "content": "[3H]dTTP was not incorporated into T4 DNA in the in vitro system for T4 DNA replication when the system was prepared from cells infected with T4 amber mutants defective in DNA replication. [3H]dTTP incorporation was resumed by adding the missing gene product to the defective system. DNA replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. By using this in vitro complementation system, molecular weights of gene 41, 43, 44, 45, and 62 products in the active form were roughly estimated as 60,000, 130,000, 130,000, 60,000, and 130,000, respectively. Complex formation between the products of genes 44 and 62 was detected. Other strong interactions between the gene products tested were not detected by glycerol density gradient sedimentation. Interaction of gene products with denatured DNA was analyzed by using a DNA-agarose column, and the results showed that products of genes 32 and 43 had a strong affinity for DNA.", "contents": "Replication of T4 DNA in vitro. II. Assay system for and some properties of gene products required for T4 DNA replication. [3H]dTTP was not incorporated into T4 DNA in the in vitro system for T4 DNA replication when the system was prepared from cells infected with T4 amber mutants defective in DNA replication. [3H]dTTP incorporation was resumed by adding the missing gene product to the defective system. DNA replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. By using this in vitro complementation system, molecular weights of gene 41, 43, 44, 45, and 62 products in the active form were roughly estimated as 60,000, 130,000, 130,000, 60,000, and 130,000, respectively. Complex formation between the products of genes 44 and 62 was detected. Other strong interactions between the gene products tested were not detected by glycerol density gradient sedimentation. Interaction of gene products with denatured DNA was analyzed by using a DNA-agarose column, and the results showed that products of genes 32 and 43 had a strong affinity for DNA.", "PMID": 972431}
{"id": "pubmed23n0032_8999", "title": "Characterization of an RNA-a protein complex isolated from the RNA bacteriophage M12.", "content": "The properties of an RNA-A protein complex isolated from the RNA bacteriophage M12 are described. The molar ratio of RNA to A protein in the complex is estimated to be 1:1. In sucrose gradients, the complex sediments like free RNA molecules. In contrast to RNA alone, which can only infect spheroplasts, the RNA-A protein complex infects intact Escherichia coli cells and produces infectious progeny particles like the original phage. Evidence is presented that the infection of the host cells by the complex takes place via F pili. All of the infectivity disappears if the ionic bonds of RNA to A protein in the complex are dissociated in 0.5 M sodium chloride buffer at 37 degrees C. Furthermore, the kinetics of complex dissociation and loss of infectivity are the same, implying that the binding of A protein to the RNA is a prerequisite for infectivity on intact host cells.", "contents": "Characterization of an RNA-a protein complex isolated from the RNA bacteriophage M12. The properties of an RNA-A protein complex isolated from the RNA bacteriophage M12 are described. The molar ratio of RNA to A protein in the complex is estimated to be 1:1. In sucrose gradients, the complex sediments like free RNA molecules. In contrast to RNA alone, which can only infect spheroplasts, the RNA-A protein complex infects intact Escherichia coli cells and produces infectious progeny particles like the original phage. Evidence is presented that the infection of the host cells by the complex takes place via F pili. All of the infectivity disappears if the ionic bonds of RNA to A protein in the complex are dissociated in 0.5 M sodium chloride buffer at 37 degrees C. Furthermore, the kinetics of complex dissociation and loss of infectivity are the same, implying that the binding of A protein to the RNA is a prerequisite for infectivity on intact host cells.", "PMID": 972432}
{"id": "pubmed23n0032_9000", "title": "Thymidine metabolism and DNA synthesis in Newcastle disease virus-infected cells.", "content": "The inhibition of thymidine incorporation into DNA in Newcastle disease virus-infected cells has been studied. At 6 h after infection of L-929 cells at high multiplicity, transport of exogenous thymidine across the cell membrane was inhibited. The kinetics of this inhibition, decreased Vmax with no change in Km, suggest that there are fewer sites available for transport in infected cells. The conversion of thymidine to dTTP was not inhibited. Equilibrium of exogenous thymidine with the acid-soluble pool occurred more slowly and at a lower level of radioactivity than in uninfected cells, and there was a reduction in the rate of incorporation of exogenous thymidine into DNA. The reduction of incorporation into the pool and into DNA was proportionate. The size of total cellular dTTP pools was changed very little in infected cells. DNA synthesized in infected cells in the presence of [3H]BrdUrd had reduced incorporation of tritium but similar buoyant density to that from uninfected cells. The results show that Newcastle disease virus inhibits DNA synthesis directly and, in addition, decreases thymidine transport. Together these account for the overall decrease in thymidine incorporation into DNA of infected cells.", "contents": "Thymidine metabolism and DNA synthesis in Newcastle disease virus-infected cells. The inhibition of thymidine incorporation into DNA in Newcastle disease virus-infected cells has been studied. At 6 h after infection of L-929 cells at high multiplicity, transport of exogenous thymidine across the cell membrane was inhibited. The kinetics of this inhibition, decreased Vmax with no change in Km, suggest that there are fewer sites available for transport in infected cells. The conversion of thymidine to dTTP was not inhibited. Equilibrium of exogenous thymidine with the acid-soluble pool occurred more slowly and at a lower level of radioactivity than in uninfected cells, and there was a reduction in the rate of incorporation of exogenous thymidine into DNA. The reduction of incorporation into the pool and into DNA was proportionate. The size of total cellular dTTP pools was changed very little in infected cells. DNA synthesized in infected cells in the presence of [3H]BrdUrd had reduced incorporation of tritium but similar buoyant density to that from uninfected cells. The results show that Newcastle disease virus inhibits DNA synthesis directly and, in addition, decreases thymidine transport. Together these account for the overall decrease in thymidine incorporation into DNA of infected cells.", "PMID": 972433}
{"id": "pubmed23n0032_9001", "title": "Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5.", "content": "Cytoplasmic RNA sequences produced in HeLa cells infected with the adeno-virus 5 temperature-sensitive mutants ts1, ts2, ts9, ts17, ts18, ts19, ts20, ts22, ts49, ts36, and ts125 were characterized by hybridization to DNA probes generated by strand separation of restriction endonuclease fragments of adenovirus 5 DNA. Two \"\"early'' mutants defective in DNA synthesis, ts125 and ts36, fail to make wild-type levels of all previously reported classes of late RNA at the nonpermissive temperature. At 40.5 degrees C, both ts125 and ts36 synthesize a wild-type complement of early cytoplasmic RNA 16 h after infection. Under these conditions, no \"\"late'' cytoplasmic RNA sequences were observed. Similarly, nuclear RNA present in these cells resembled early cytoplasmic RNA rather than late nuclear RNA. All the late adenovirus 5 temperature-sensitive mutants synthesized normal wild-type levels of late cytoplasmic RNA at the nonpermissive temperature, except ts2, which appears to overproduce certain cytoplasmic species.", "contents": "Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5. Cytoplasmic RNA sequences produced in HeLa cells infected with the adeno-virus 5 temperature-sensitive mutants ts1, ts2, ts9, ts17, ts18, ts19, ts20, ts22, ts49, ts36, and ts125 were characterized by hybridization to DNA probes generated by strand separation of restriction endonuclease fragments of adenovirus 5 DNA. Two \"\"early'' mutants defective in DNA synthesis, ts125 and ts36, fail to make wild-type levels of all previously reported classes of late RNA at the nonpermissive temperature. At 40.5 degrees C, both ts125 and ts36 synthesize a wild-type complement of early cytoplasmic RNA 16 h after infection. Under these conditions, no \"\"late'' cytoplasmic RNA sequences were observed. Similarly, nuclear RNA present in these cells resembled early cytoplasmic RNA rather than late nuclear RNA. All the late adenovirus 5 temperature-sensitive mutants synthesized normal wild-type levels of late cytoplasmic RNA at the nonpermissive temperature, except ts2, which appears to overproduce certain cytoplasmic species.", "PMID": 972434}
{"id": "pubmed23n0032_9002", "title": "Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate.", "content": "A calicivirus, San Miguel sea lion virus serotype 4, isolate 15FT, externally labelled with 125I, was shown by gel electrophoresis to possess a single major polypeptide. The polypeptide migrated anomalously upon electrophoresis in two sodium dodecyl sulfate (SDS) systems: more slowly than bovine serum albumin in a continuous phosphate-buffered system and more rapidly than bovine serum albumin in a discontinuous system. Estimated molecular weights in the two systems were approximately 71,000 and 64,000, respectively. There was no clear evidence for a minor virion polypeptide. Treatment of purified San Miguel sea lion virions with dimethyl suberimidate, a cross-linking reagent, preserved virion integrity during long-term storage at 4 degrees C. Oligomeric species of the polypeptide were observed upon electrophoresis of products from cross-linked virions. Based upon a preferred polypeptide molecular weight estimate of 71,000 and distribution of oligomeric species, a calicivirion model with 120 monomeric protein units is proposed as an alternative to a 180-unit model.", "contents": "Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate. A calicivirus, San Miguel sea lion virus serotype 4, isolate 15FT, externally labelled with 125I, was shown by gel electrophoresis to possess a single major polypeptide. The polypeptide migrated anomalously upon electrophoresis in two sodium dodecyl sulfate (SDS) systems: more slowly than bovine serum albumin in a continuous phosphate-buffered system and more rapidly than bovine serum albumin in a discontinuous system. Estimated molecular weights in the two systems were approximately 71,000 and 64,000, respectively. There was no clear evidence for a minor virion polypeptide. Treatment of purified San Miguel sea lion virions with dimethyl suberimidate, a cross-linking reagent, preserved virion integrity during long-term storage at 4 degrees C. Oligomeric species of the polypeptide were observed upon electrophoresis of products from cross-linked virions. Based upon a preferred polypeptide molecular weight estimate of 71,000 and distribution of oligomeric species, a calicivirion model with 120 monomeric protein units is proposed as an alternative to a 180-unit model.", "PMID": 972435}
{"id": "pubmed23n0032_9003", "title": "Structural proteins of La Crosse virus.", "content": "Preparations of La Crosse virus, a member of the California encephalitis group of bunyaviruses, were found to possess three major virion proteins. Two of the proteins were glycosylated (G1 and G2) and were located on the surface of the virus particles. These two glycoproteins were present in equimolar amounts and possessed apparent molecular weights of 120 X 10(3) and 34 X 10(3). Virion nucleocapsids, isolated by a nonionic detergent and salt treatment, contained another major protein, N (molecular weight = 23 X 10(3)). A large, but minor, protein species L (molecular weight = 180 X 10(3)) was also found in virus preparations. The approximate number of protein molecules per virion has been determined. Electron microscopy of purified La Crosse virus indicated that the virus particle (mean diameter, 91 nm) is enveloped and possesses irregular surface projections (length, 10 nm).", "contents": "Structural proteins of La Crosse virus. Preparations of La Crosse virus, a member of the California encephalitis group of bunyaviruses, were found to possess three major virion proteins. Two of the proteins were glycosylated (G1 and G2) and were located on the surface of the virus particles. These two glycoproteins were present in equimolar amounts and possessed apparent molecular weights of 120 X 10(3) and 34 X 10(3). Virion nucleocapsids, isolated by a nonionic detergent and salt treatment, contained another major protein, N (molecular weight = 23 X 10(3)). A large, but minor, protein species L (molecular weight = 180 X 10(3)) was also found in virus preparations. The approximate number of protein molecules per virion has been determined. Electron microscopy of purified La Crosse virus indicated that the virus particle (mean diameter, 91 nm) is enveloped and possesses irregular surface projections (length, 10 nm).", "PMID": 972436}
{"id": "pubmed23n0032_9004", "title": "Methylation pattern of genomic RNA from Moloney murine leukemia virus.", "content": "32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.", "contents": "Methylation pattern of genomic RNA from Moloney murine leukemia virus. 32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.", "PMID": 972437}
{"id": "pubmed23n0032_9005", "title": "The influence of ventricular function on the results of aortic valve replacement for aortic stenosis.", "content": "Thirty-three patients who had aortic valve replacement (AVR) for isolated aortic stenosis (AS) were retrospectively evaluated to determine the influence of preoperative ventricular function on the results of operation. Patients were arbitrarily divided by ejection fraction (EF) into Group I having EF 0.46 or above and Group II having EF 0.45 or below. Group II had significantly lower mean EF and cardiac index and higher left ventricular end-diastolic volume index and end-diastolic pressure. There were two early and no late deaths in 21 Group I patients. There were no early and five late deaths in 12 Group II patients. All Group I survivors and six of 12 Group II patients were New York Heart Association (NYHA) functional Class I or II at follow-up evaluation. No preoperative hemodynamic index was predictive of which Group II patients would do well. Mortality was substantially higher in patients with severe preoperative disability. Early or late death occurred in five of 12 patients who were NYHA functional Class IV before operation but in only two of 21 patients who were functional Class II or III preoperatively. We conclude that patients with isolated AS and poor left ventricular function have a low hospital mortality but an increased risk of late death or poor functional result following AVR. Excellent functional results can be obtained after AVR in most patients with AS and good left ventricular function and approximately half of the patients with AS and poor left ventricular function.", "contents": "The influence of ventricular function on the results of aortic valve replacement for aortic stenosis. Thirty-three patients who had aortic valve replacement (AVR) for isolated aortic stenosis (AS) were retrospectively evaluated to determine the influence of preoperative ventricular function on the results of operation. Patients were arbitrarily divided by ejection fraction (EF) into Group I having EF 0.46 or above and Group II having EF 0.45 or below. Group II had significantly lower mean EF and cardiac index and higher left ventricular end-diastolic volume index and end-diastolic pressure. There were two early and no late deaths in 21 Group I patients. There were no early and five late deaths in 12 Group II patients. All Group I survivors and six of 12 Group II patients were New York Heart Association (NYHA) functional Class I or II at follow-up evaluation. No preoperative hemodynamic index was predictive of which Group II patients would do well. Mortality was substantially higher in patients with severe preoperative disability. Early or late death occurred in five of 12 patients who were NYHA functional Class IV before operation but in only two of 21 patients who were functional Class II or III preoperatively. We conclude that patients with isolated AS and poor left ventricular function have a low hospital mortality but an increased risk of late death or poor functional result following AVR. Excellent functional results can be obtained after AVR in most patients with AS and good left ventricular function and approximately half of the patients with AS and poor left ventricular function.", "PMID": 972440}
{"id": "pubmed23n0032_9006", "title": "Role of monovalent ions in the reabsorption of fluid by isolated perfused proximal renal tubules of the rabbit.", "content": "Proximal convoluted tubules were dissected from rabbit kidneys and perfused in vitro in order to determine the effect of monovalent ions on fluid absorption and transepithelial voltage. Replacement of sodium in the perfusate and bath by lithium, tetramethyl ammonium or choline caused the rate of fluid absorption and voltage to fall to near zero. Replacement of potassium in the bath by sodium had the identical effect. Replacement of chloride by nitrate or perchlorate had comparatively little effect. The results are consistent with the generally held view that active sodium transport (mediated by a Na- and K- activated adenosine triphosphatase) is the primary process responsible for the absorption of the fluid and the voltage. Replacement of bicarbonate in the perfusate and bath by chloride caused the rate of fluid absorption to decrease by 33%. The possible relation between sodium transport and bicarbonate is discussed.", "contents": "Role of monovalent ions in the reabsorption of fluid by isolated perfused proximal renal tubules of the rabbit. Proximal convoluted tubules were dissected from rabbit kidneys and perfused in vitro in order to determine the effect of monovalent ions on fluid absorption and transepithelial voltage. Replacement of sodium in the perfusate and bath by lithium, tetramethyl ammonium or choline caused the rate of fluid absorption and voltage to fall to near zero. Replacement of potassium in the bath by sodium had the identical effect. Replacement of chloride by nitrate or perchlorate had comparatively little effect. The results are consistent with the generally held view that active sodium transport (mediated by a Na- and K- activated adenosine triphosphatase) is the primary process responsible for the absorption of the fluid and the voltage. Replacement of bicarbonate in the perfusate and bath by chloride caused the rate of fluid absorption to decrease by 33%. The possible relation between sodium transport and bicarbonate is discussed.", "PMID": 972442}
{"id": "pubmed23n0032_9007", "title": "Influence of volume expansion on NaC1 reabsorption in the diluting segments of the nephron: a study using clearance methods.", "content": "Whether volume expansion influences NaC1 reabsorption by the diluting segment of the nephron remains a matter of controversy. In the present studies this question has been examined in normal unanesthetized dogs, undergoing maximal water diuresis. Free water clearance (CH2O/GFR) has been used as the index of NaC1 reabsorption in the diluting segment. Three expressions have been employed for \"distal delivery\" of NaC1: a) V/GFR, designated as the \"volume term\"; b) (CNa/GFR + CH2O/GFR), the \"sodium term;\" and c) (CC1/GFR + CH2O/GFR), the \"chloride term\". The validity of these terms is discussed. Three techniques were used to increase distal delivery: 1) the administration of acetazolamide to dogs in which extracellular fluid (ECF) volume was not expanded (grop 1); 2) \"moderate\" volume expansion (group 2); and 3) \"marked\" volume expansion (group 3). CH2O/GFR increased progressively with rising values for \"distal delivery\" regardless of which term was used to calculate the latter. With all three delivery terms, differences in distal NaC1 reabsorption emerged between the two volume-expanded groups, though only with the \"chloride\" term did substantial differences also emerge between the nonexpanded group 1 dogs and both volume-expanded groups. In group 1, values for CH2O/GFR increased in close to a linear fashion up to distal delivery values equal to 24% of the volume of glomerular filtrate. However, at high rates of distal delivery the rate of rise of CH2O/GFR was less in group 2 than in group 1 and the depression of values was even greater in group 3. Within the limits of the techniques used, the data suggest that volume expansion inhibits fractional NaC1 reabsorption in the diluting segment of the nephron in a dose-related fashion. The \"chloride\" term was found to be superior to the \"volume\" and \"sodium\" terms in revealing these changes.", "contents": "Influence of volume expansion on NaC1 reabsorption in the diluting segments of the nephron: a study using clearance methods. Whether volume expansion influences NaC1 reabsorption by the diluting segment of the nephron remains a matter of controversy. In the present studies this question has been examined in normal unanesthetized dogs, undergoing maximal water diuresis. Free water clearance (CH2O/GFR) has been used as the index of NaC1 reabsorption in the diluting segment. Three expressions have been employed for \"distal delivery\" of NaC1: a) V/GFR, designated as the \"volume term\"; b) (CNa/GFR + CH2O/GFR), the \"sodium term;\" and c) (CC1/GFR + CH2O/GFR), the \"chloride term\". The validity of these terms is discussed. Three techniques were used to increase distal delivery: 1) the administration of acetazolamide to dogs in which extracellular fluid (ECF) volume was not expanded (grop 1); 2) \"moderate\" volume expansion (group 2); and 3) \"marked\" volume expansion (group 3). CH2O/GFR increased progressively with rising values for \"distal delivery\" regardless of which term was used to calculate the latter. With all three delivery terms, differences in distal NaC1 reabsorption emerged between the two volume-expanded groups, though only with the \"chloride\" term did substantial differences also emerge between the nonexpanded group 1 dogs and both volume-expanded groups. In group 1, values for CH2O/GFR increased in close to a linear fashion up to distal delivery values equal to 24% of the volume of glomerular filtrate. However, at high rates of distal delivery the rate of rise of CH2O/GFR was less in group 2 than in group 1 and the depression of values was even greater in group 3. Within the limits of the techniques used, the data suggest that volume expansion inhibits fractional NaC1 reabsorption in the diluting segment of the nephron in a dose-related fashion. The \"chloride\" term was found to be superior to the \"volume\" and \"sodium\" terms in revealing these changes.", "PMID": 972443}
{"id": "pubmed23n0032_9008", "title": "Variations in muscle cell protein of severely uremic children.", "content": "Alkali-solution protein (ASP) was determined in the muscle of 17 controls and 32 uremic children including 9 patients receiving intermittent hemodialysis. With regard to fat-free solids content (FFS), ASP values were found to be lower in uremic children than in normal subjects (59.2 +/- 1.6 vs. 76.4 +/- 1.9 g/100 g of FFS). This decrease was greater in hemodialyzed children (53.8 +/- 1.6 g/100 g of FFS). ASP depletion was found also when the results were expressed in relation to muscle potassium and phosphorus and therefore appeared to be due to an actual decrease in the amount of proteins per cell. ASP values were especially low (52.2 +/- 2.8 g/100 g of FFS) in seven nondialyzed children receiving a low protein diet (less than 0.165 g of N/kg of body wt/day) and in patients with a height below 2 SD (56.3 +/- 3.1 g/100 g of FFS). In the nine cases of glomerulonephritis, loss of muscle protein sfollowed plasma protein depletion. A relationship was found between plasma transferrin concentration and ASP. ASP depletion seemed to be related to the level of renal insufficiency. However, the most important factor in ASP depletion was probably an inadequate protein intake associated with the possible metabolic effects of uremix toxins which are currently unknown.", "contents": "Variations in muscle cell protein of severely uremic children. Alkali-solution protein (ASP) was determined in the muscle of 17 controls and 32 uremic children including 9 patients receiving intermittent hemodialysis. With regard to fat-free solids content (FFS), ASP values were found to be lower in uremic children than in normal subjects (59.2 +/- 1.6 vs. 76.4 +/- 1.9 g/100 g of FFS). This decrease was greater in hemodialyzed children (53.8 +/- 1.6 g/100 g of FFS). ASP depletion was found also when the results were expressed in relation to muscle potassium and phosphorus and therefore appeared to be due to an actual decrease in the amount of proteins per cell. ASP values were especially low (52.2 +/- 2.8 g/100 g of FFS) in seven nondialyzed children receiving a low protein diet (less than 0.165 g of N/kg of body wt/day) and in patients with a height below 2 SD (56.3 +/- 3.1 g/100 g of FFS). In the nine cases of glomerulonephritis, loss of muscle protein sfollowed plasma protein depletion. A relationship was found between plasma transferrin concentration and ASP. ASP depletion seemed to be related to the level of renal insufficiency. However, the most important factor in ASP depletion was probably an inadequate protein intake associated with the possible metabolic effects of uremix toxins which are currently unknown.", "PMID": 972444}
{"id": "pubmed23n0032_9009", "title": "Effect of dialysate calcium concentration on bone disease in patients on hemodialysis.", "content": "Plasma biochemistry, bone radiology and morphometry were studied in a group of 20 patients receiving maintenance hemodialysis. The aim was to determine if increasing the dialysate calcium concentration would decrease plasma parathyroid hormone and improve the radiologic appearances of bone in patients without producing serious side effects. Dialysate calcium concentration was increased stepwise from 4.5 to 6.0 and then to 7.0 mg/100 ml. Mean predialysis plasma calcium concentration increased from 9.4 to 9.7 and then to 10.0 mg/100 ml and mean predialysis phosphate concentration increased from 5.3 to 5.6 mg/100 ml. Parathyroid hormone concentration was elevated in all patients but the mean concentration did not change significantly although in seven patients a decrease occurred. Six patients had radiologic signs of renal bone disease, two patients showed improvement and three patients developed bone disease during the study. The patients with radiologic bone disease had the highest parathyroid hormone concentrations and the majority were female. Morphometric bone measurements showed that bone loss during the study occurred mainly in the male patients. Soft tissue calcification continued to appear during the study. Although the response to some patients to an increased dialysate calcium concentration was favorable, it was impossible to predict which types of patient would benefit from the use of a dialysate calcium concentration of 7.0 mg/100 ml.", "contents": "Effect of dialysate calcium concentration on bone disease in patients on hemodialysis. Plasma biochemistry, bone radiology and morphometry were studied in a group of 20 patients receiving maintenance hemodialysis. The aim was to determine if increasing the dialysate calcium concentration would decrease plasma parathyroid hormone and improve the radiologic appearances of bone in patients without producing serious side effects. Dialysate calcium concentration was increased stepwise from 4.5 to 6.0 and then to 7.0 mg/100 ml. Mean predialysis plasma calcium concentration increased from 9.4 to 9.7 and then to 10.0 mg/100 ml and mean predialysis phosphate concentration increased from 5.3 to 5.6 mg/100 ml. Parathyroid hormone concentration was elevated in all patients but the mean concentration did not change significantly although in seven patients a decrease occurred. Six patients had radiologic signs of renal bone disease, two patients showed improvement and three patients developed bone disease during the study. The patients with radiologic bone disease had the highest parathyroid hormone concentrations and the majority were female. Morphometric bone measurements showed that bone loss during the study occurred mainly in the male patients. Soft tissue calcification continued to appear during the study. Although the response to some patients to an increased dialysate calcium concentration was favorable, it was impossible to predict which types of patient would benefit from the use of a dialysate calcium concentration of 7.0 mg/100 ml.", "PMID": 972445}
{"id": "pubmed23n0032_9010", "title": "Some factors affecting the likelihood of moderate drinking by treated alcoholics.", "content": "The 1-year outcome in alcoholics who participated in an outpatient alcoholism treatment program which stressed moderate drinking as the ultimate treatment goal was investigated. The results suggest that such an approach, in an otherwise conventional treatment program, is likely to result in a higher rate of moderate drinking and a lower rate of abstinence but not in a higher over-all recovery rate than a total-abstinence orientation.", "contents": "Some factors affecting the likelihood of moderate drinking by treated alcoholics. The 1-year outcome in alcoholics who participated in an outpatient alcoholism treatment program which stressed moderate drinking as the ultimate treatment goal was investigated. The results suggest that such an approach, in an otherwise conventional treatment program, is likely to result in a higher rate of moderate drinking and a lower rate of abstinence but not in a higher over-all recovery rate than a total-abstinence orientation.", "PMID": 972537}
{"id": "pubmed23n0032_9011", "title": "Assessing alcoholism treatment effectiveness. A comparison of three evaluative measures.", "content": "The success rates of patients in an alcoholism treatment unit were evaluated according to three criteria: vocational rehabilitation, abstinence and a behavioral rating scale. The three measures yielded success rates of 70, 34 and 62%, respectively.", "contents": "Assessing alcoholism treatment effectiveness. A comparison of three evaluative measures. The success rates of patients in an alcoholism treatment unit were evaluated according to three criteria: vocational rehabilitation, abstinence and a behavioral rating scale. The three measures yielded success rates of 70, 34 and 62%, respectively.", "PMID": 972538}
{"id": "pubmed23n0032_9012", "title": "Prognostic indicators for black and white alcoholics in the U.S. Navy.", "content": "Among 132 Black and 1384 White Navy enlisted men, about the same proportion of each group (76 and 77%) returned to effective duty after treatment for alcoholism. The best predictors of the sailors' effective recovery after treatment were favorable prognoses by treatment personnel, and their being promoted above the lowest grades of pay.", "contents": "Prognostic indicators for black and white alcoholics in the U.S. Navy. Among 132 Black and 1384 White Navy enlisted men, about the same proportion of each group (76 and 77%) returned to effective duty after treatment for alcoholism. The best predictors of the sailors' effective recovery after treatment were favorable prognoses by treatment personnel, and their being promoted above the lowest grades of pay.", "PMID": 972539}
{"id": "pubmed23n0032_9013", "title": "Referral to alcoholism programs in industry. Coercion, confrontation and choice.", "content": "Employees highly coerced into entering industrial alcoholism programs because of affected job performance reported a higher proportion of work improvement than those in treatment for other reasons.", "contents": "Referral to alcoholism programs in industry. Coercion, confrontation and choice. Employees highly coerced into entering industrial alcoholism programs because of affected job performance reported a higher proportion of work improvement than those in treatment for other reasons.", "PMID": 972540}
{"id": "pubmed23n0032_9014", "title": "Locus of control among alcoholics. Some empirical and conceptual issues.", "content": "Research on locus of control of reinforcement of alcoholics is reviewed. The results are unclear, and a need for the further study of the construct validity of Rotter's Locus of Control Scale, as applied to alcoholics, is demonstrated.", "contents": "Locus of control among alcoholics. Some empirical and conceptual issues. Research on locus of control of reinforcement of alcoholics is reviewed. The results are unclear, and a need for the further study of the construct validity of Rotter's Locus of Control Scale, as applied to alcoholics, is demonstrated.", "PMID": 972541}
{"id": "pubmed23n0032_9015", "title": "Aggressive behavior of intoxicated subjects. The effect of third-party intervention.", "content": "Intoxicated and nonintoxicated subjects competed in a reaction-time situation in the presence of either a pressuring observer or a silent observer. The intoxicated subjects initiated a higher level of attack than the nonintoxicated subjects. The social pressure manipulation was effective in reducing the aggressive behavior of both groups.", "contents": "Aggressive behavior of intoxicated subjects. The effect of third-party intervention. Intoxicated and nonintoxicated subjects competed in a reaction-time situation in the presence of either a pressuring observer or a silent observer. The intoxicated subjects initiated a higher level of attack than the nonintoxicated subjects. The social pressure manipulation was effective in reducing the aggressive behavior of both groups.", "PMID": 972542}
{"id": "pubmed23n0032_9016", "title": "Impressions of Jewish alcoholics.", "content": "Jews, constituting 6% of the adult population in Toronto, contributed 0.5% of all admissions to one private and one public clinic for alcoholism during a 10-year period. Their demographic, sociocultural and psychological characteristics, and several modes of adjustment to being Jewish alcoholics, are described.", "contents": "Impressions of Jewish alcoholics. Jews, constituting 6% of the adult population in Toronto, contributed 0.5% of all admissions to one private and one public clinic for alcoholism during a 10-year period. Their demographic, sociocultural and psychological characteristics, and several modes of adjustment to being Jewish alcoholics, are described.", "PMID": 972543}
{"id": "pubmed23n0032_9017", "title": "Shifts in the water and alcohol solution intake by rats under conditions of schedule induction.", "content": "Choice between a 5% alcohol solution and water by rats was labile when both were drunk to excess under conditions of schedule induction. Selection of the two fluids could be stabilized by sweetening the alcohol solution with saccharin.", "contents": "Shifts in the water and alcohol solution intake by rats under conditions of schedule induction. Choice between a 5% alcohol solution and water by rats was labile when both were drunk to excess under conditions of schedule induction. Selection of the two fluids could be stabilized by sweetening the alcohol solution with saccharin.", "PMID": 972544}
{"id": "pubmed23n0032_9018", "title": "Alcohol consumption by rat and mouse strains. Functions of taste and alcohol deprivation.", "content": "Three groups of animals (two of rats and one of mice) were tested to determine the effects of alcohol deprivation and the addition of sweetener to an alcohol solution on the animals' intake of alcohol.", "contents": "Alcohol consumption by rat and mouse strains. Functions of taste and alcohol deprivation. Three groups of animals (two of rats and one of mice) were tested to determine the effects of alcohol deprivation and the addition of sweetener to an alcohol solution on the animals' intake of alcohol.", "PMID": 972545}
{"id": "pubmed23n0032_9019", "title": "Effects of ethanol intake on the immune system of guinea pigs.", "content": "Groups of guinea pigs were maintained with alcohol solutions not sufficient to result in liver dysfunction prior to and during investigations designed to determine whether ethanol would nonetheless impair cell-mediated or humoral immunity. No impairment of either was observed in any of the experimental groups.", "contents": "Effects of ethanol intake on the immune system of guinea pigs. Groups of guinea pigs were maintained with alcohol solutions not sufficient to result in liver dysfunction prior to and during investigations designed to determine whether ethanol would nonetheless impair cell-mediated or humoral immunity. No impairment of either was observed in any of the experimental groups.", "PMID": 972546}
{"id": "pubmed23n0032_9020", "title": "Alcohol education for alcoholics. Relation to attitude changes and posttreatment abstinence.", "content": "Test results of 98 inpatient alcoholics revealed that improvements in attitudes toward treatment were positively related to gains in some areas of knowledge of alcoholism, but a gain in knowledge was not related to posthospital abstinence.", "contents": "Alcohol education for alcoholics. Relation to attitude changes and posttreatment abstinence. Test results of 98 inpatient alcoholics revealed that improvements in attitudes toward treatment were positively related to gains in some areas of knowledge of alcoholism, but a gain in knowledge was not related to posthospital abstinence.", "PMID": 972547}
{"id": "pubmed23n0032_9021", "title": "Induction of alcohol selection in laboratory rats by ultraviolet light.", "content": "Rats exposed to ultraviolet light with maximum emission at 350 nm showed a stable intake of alcohol over water. A control group of animals exposed to white fluorescence light did not drink more alcohol than water.", "contents": "Induction of alcohol selection in laboratory rats by ultraviolet light. Rats exposed to ultraviolet light with maximum emission at 350 nm showed a stable intake of alcohol over water. A control group of animals exposed to white fluorescence light did not drink more alcohol than water.", "PMID": 972548}
{"id": "pubmed23n0032_9022", "title": "Disulfiram-induced convulsions without challenge by alcohol.", "content": "A patient who had several grand-mal seizures while taking disulfiram in the absence of a challenge with alcohol is described and some of the possible mechanisms for this occurrence are discussed.", "contents": "Disulfiram-induced convulsions without challenge by alcohol. A patient who had several grand-mal seizures while taking disulfiram in the absence of a challenge with alcohol is described and some of the possible mechanisms for this occurrence are discussed.", "PMID": 972549}
{"id": "pubmed23n0032_9023", "title": "Alcoholism, cigarette smoking, coffee drinking and extraversion.", "content": "Alcoholic patients smoked significantly more cigarettes and drank more cups of coffee than nonalcoholic psychiatric patients even when the higher extraversion scores of the alcoholics, as measured by the Eysenck Personality Inventory, were statistically controlled.", "contents": "Alcoholism, cigarette smoking, coffee drinking and extraversion. Alcoholic patients smoked significantly more cigarettes and drank more cups of coffee than nonalcoholic psychiatric patients even when the higher extraversion scores of the alcoholics, as measured by the Eysenck Personality Inventory, were statistically controlled.", "PMID": 972550}
{"id": "pubmed23n0032_9024", "title": "Drinking outcome of specific alcoholic subgroups.", "content": "Alcoholic's scores on the Eysenck Personality Inventory, the Drinking Behavior Inventory, the MacAndre Alcoholism Scale and Rohan's classification system for alcoholics were not related to drinking behavior during or after a treatment program that permitted drinking.", "contents": "Drinking outcome of specific alcoholic subgroups. Alcoholic's scores on the Eysenck Personality Inventory, the Drinking Behavior Inventory, the MacAndre Alcoholism Scale and Rohan's classification system for alcoholics were not related to drinking behavior during or after a treatment program that permitted drinking.", "PMID": 972551}
{"id": "pubmed23n0032_9025", "title": "Self-esteem in alcoholics and nonalcoholics.", "content": "Alcoholics scored lower on a test of self-esteem than did nonalcoholics, Alcoholics with lower self-esteem were found to be more willing to seek treatment than those with higher self-esteem.", "contents": "Self-esteem in alcoholics and nonalcoholics. Alcoholics scored lower on a test of self-esteem than did nonalcoholics, Alcoholics with lower self-esteem were found to be more willing to seek treatment than those with higher self-esteem.", "PMID": 972552}
{"id": "pubmed23n0032_9026", "title": "Compliance techniques to increase mailed questionnaire return rates from alcoholics.", "content": "The effects of three compliance techniques were examined. None elicited more returns than did a conventional mailed questionnaire.", "contents": "Compliance techniques to increase mailed questionnaire return rates from alcoholics. The effects of three compliance techniques were examined. None elicited more returns than did a conventional mailed questionnaire.", "PMID": 972553}
{"id": "pubmed23n0032_9027", "title": "A geographical analysis of drunken drivers.", "content": "The \"nearest-neighbor\" spatial approach proved a useful tool in defining the geographic distribution pattern of persons arrested for drunken driving.", "contents": "A geographical analysis of drunken drivers. The \"nearest-neighbor\" spatial approach proved a useful tool in defining the geographic distribution pattern of persons arrested for drunken driving.", "PMID": 972554}
{"id": "pubmed23n0032_9028", "title": "Studying the impact of patient drug profiles in an HMO.", "content": "The objectives of the study were to determine if a drug profile quantitatively and qualitatively influenced various aspects of drug utilization. A group of 1,632 medically indigent persons enrolled in the Kaiser Health Plan constituted the study population. This population received completely prepaid medical care including drug services from the Kaiser Medical Care Program. A computer-based chronological and monthly updated listing of drugs ordered and prescriptions received was provided as the front page of the centralized outpatient medical chart for a randomly selected half of the study population for a full year. Duplicate and bound copies of these patient drug profiles were also provided each outpatient pharmacy. The findings indicated the presence of the drug profile did not quantitatively or qualitatively affect prescription receipt of the group with the profile. Although the findings must be viewed in the contexts of the population served and the medical care setting, the findings strongly indicate the need for additional research to assess additional forms of drug profiles and under conditions not addressed by this study.", "contents": "Studying the impact of patient drug profiles in an HMO. The objectives of the study were to determine if a drug profile quantitatively and qualitatively influenced various aspects of drug utilization. A group of 1,632 medically indigent persons enrolled in the Kaiser Health Plan constituted the study population. This population received completely prepaid medical care including drug services from the Kaiser Medical Care Program. A computer-based chronological and monthly updated listing of drugs ordered and prescriptions received was provided as the front page of the centralized outpatient medical chart for a randomly selected half of the study population for a full year. Duplicate and bound copies of these patient drug profiles were also provided each outpatient pharmacy. The findings indicated the presence of the drug profile did not quantitatively or qualitatively affect prescription receipt of the group with the profile. Although the findings must be viewed in the contexts of the population served and the medical care setting, the findings strongly indicate the need for additional research to assess additional forms of drug profiles and under conditions not addressed by this study.", "PMID": 972558}
{"id": "pubmed23n0032_9029", "title": "The role of group practice in the distribution of physicians in nonmetropolitan areas.", "content": "The geographic maldistribution of physicians persists as a major obstacle to improving the availability of health services in rural areas. This study looks at group practice as a potential factor in the location of physicians in nonmetropolitan areas. The basic assumptions are 1) the expected conditions of practice are critical to a physician's decision about where to locate, and 2) a group practice form of organization alleviates many of the deterrents to rural practice. The sample for the study is 287 nonmetropolitan trade areas in eight geographic regions of the U.S. Stepwise multiple regression is used to evaluate the relationship of demographic and health system variables, including group practice, to the physician/population ratio. Analyses are done for 1960 and 1970 and then for the change over the ten-year time period. The regression outcomes show that income, population over age 64, and urbanization are most helpful in explaining physician distribution in 1960. In 1970, hospital facilities also contribute. However, the percentage change in the physician/population ratio between 1960 and 1970 is explained by the 1960 physician/population ratio and the per cent of physicians in group practice in 1960. The findings suggest that attractive practice arrangements may be one way to alter the geographic maldistribution of physicians.", "contents": "The role of group practice in the distribution of physicians in nonmetropolitan areas. The geographic maldistribution of physicians persists as a major obstacle to improving the availability of health services in rural areas. This study looks at group practice as a potential factor in the location of physicians in nonmetropolitan areas. The basic assumptions are 1) the expected conditions of practice are critical to a physician's decision about where to locate, and 2) a group practice form of organization alleviates many of the deterrents to rural practice. The sample for the study is 287 nonmetropolitan trade areas in eight geographic regions of the U.S. Stepwise multiple regression is used to evaluate the relationship of demographic and health system variables, including group practice, to the physician/population ratio. Analyses are done for 1960 and 1970 and then for the change over the ten-year time period. The regression outcomes show that income, population over age 64, and urbanization are most helpful in explaining physician distribution in 1960. In 1970, hospital facilities also contribute. However, the percentage change in the physician/population ratio between 1960 and 1970 is explained by the 1960 physician/population ratio and the per cent of physicians in group practice in 1960. The findings suggest that attractive practice arrangements may be one way to alter the geographic maldistribution of physicians.", "PMID": 972559}
{"id": "pubmed23n0032_9030", "title": "Time utilization of a population of general surgeons in a prepaid group practice.", "content": "Seven general surgeons in a prepaid group practice previously shown to have a mean operative work load of 9.2 hernia equivalents (HE) per week were found to have a standardized mean daytime working week of 56.2 hours, exclusive of evening activities of which 50.7 hours were devoted to professional activities. The surgeons also devoted a mean of 6.7 evening hours per week to professional activities for a mean net professional week of 57.4 hours. Comparisons with a population of previously studied community surgeons revealed that the prepaid group surgeons were able to produce a surgical output more than double that of the community surgeons while devoting only one and a half as much time to professional activities. Economies in the utilization of surgical manpower in the prepaid group appear to stem from: 1) restriction of practice setting to a single geographic location, 2) restriction of patients to surgical patients, 3) reduced surgeon waiting time in the office, and 4) the utilization of paraprofessional personnel for selected operative assisting. These economies were achieved while the prepaid group surgeons were observed to average more time per patient visit both on rounds and in the office than the community surgeons.", "contents": "Time utilization of a population of general surgeons in a prepaid group practice. Seven general surgeons in a prepaid group practice previously shown to have a mean operative work load of 9.2 hernia equivalents (HE) per week were found to have a standardized mean daytime working week of 56.2 hours, exclusive of evening activities of which 50.7 hours were devoted to professional activities. The surgeons also devoted a mean of 6.7 evening hours per week to professional activities for a mean net professional week of 57.4 hours. Comparisons with a population of previously studied community surgeons revealed that the prepaid group surgeons were able to produce a surgical output more than double that of the community surgeons while devoting only one and a half as much time to professional activities. Economies in the utilization of surgical manpower in the prepaid group appear to stem from: 1) restriction of practice setting to a single geographic location, 2) restriction of patients to surgical patients, 3) reduced surgeon waiting time in the office, and 4) the utilization of paraprofessional personnel for selected operative assisting. These economies were achieved while the prepaid group surgeons were observed to average more time per patient visit both on rounds and in the office than the community surgeons.", "PMID": 972560}
{"id": "pubmed23n0032_9031", "title": "Analyzing the start-up effects of new patients on an ambulatory care program.", "content": "Holmes County, Mississippi was the site of a pilot study to develop a community-based program to combat hypertension, the primary health problem in the rural black community. Using experience gained during the pilot program, a model was developed that computes, over a sequence of planning periods, the personnel utilization and the treatment status of the patients as functions of treatment protocols, allocations of procedures to personnel, and the additions of new patients. The model can be used to study the impact of new patients on any ambulatory care program or any health maintenance organization in which there is unusually high personnel resource utilization during a patients first visit.", "contents": "Analyzing the start-up effects of new patients on an ambulatory care program. Holmes County, Mississippi was the site of a pilot study to develop a community-based program to combat hypertension, the primary health problem in the rural black community. Using experience gained during the pilot program, a model was developed that computes, over a sequence of planning periods, the personnel utilization and the treatment status of the patients as functions of treatment protocols, allocations of procedures to personnel, and the additions of new patients. The model can be used to study the impact of new patients on any ambulatory care program or any health maintenance organization in which there is unusually high personnel resource utilization during a patients first visit.", "PMID": 972561}
{"id": "pubmed23n0032_9032", "title": "Satisfaction with health services: some policy implications.", "content": "Although consumer satisfaction with health services is a frequently measured variable in health services research, the relevance of this variable for health policy is not always clear. The present paper suggests two criteria for specifying the relevance of satisfaction with health services for policy at the organizational level. First, it should be shown that consumer satisfaction is the result of organizational behaviors that are manipulable by policy. second, where he has a choice, it should be shown that satisfaction affects the organizationally relevant behavior of the consumer. Data are presented that support both criteria. Several suggestions are made for maximizing the policy relevance of future studies of satisfaction.", "contents": "Satisfaction with health services: some policy implications. Although consumer satisfaction with health services is a frequently measured variable in health services research, the relevance of this variable for health policy is not always clear. The present paper suggests two criteria for specifying the relevance of satisfaction with health services for policy at the organizational level. First, it should be shown that consumer satisfaction is the result of organizational behaviors that are manipulable by policy. second, where he has a choice, it should be shown that satisfaction affects the organizationally relevant behavior of the consumer. Data are presented that support both criteria. Several suggestions are made for maximizing the policy relevance of future studies of satisfaction.", "PMID": 972562}
{"id": "pubmed23n0032_9033", "title": "Nursing homes and the Roemer-Feldstein hypothesis.", "content": "The theory of certification-of-need is based in large part on the Roemer-Feldstein hypothesis, which holds that demand for services is proportional to bed supply. Athough developed solely from hospital data, this hypothesis is commonly assumed to hold for nursing homes as well. In fact, a high correlation between nursing home bed supply and bed utilization does exist. However, the rate of utilization should not be judged without reference to the appropriateness of use. Analysis of nursing home placement data revealed that overplacement of patients in skilled nursing facilities decreased as bed supply increased. These results suggest that the Roemer-Feldstein hypothesis may provide an inadequate rationale for regulation of nursing home bed supply. Further developments should be based on more sophisticated notions of bed supply, should make reference to the appropriateness of services, and should be subjected to empirical verification.", "contents": "Nursing homes and the Roemer-Feldstein hypothesis. The theory of certification-of-need is based in large part on the Roemer-Feldstein hypothesis, which holds that demand for services is proportional to bed supply. Athough developed solely from hospital data, this hypothesis is commonly assumed to hold for nursing homes as well. In fact, a high correlation between nursing home bed supply and bed utilization does exist. However, the rate of utilization should not be judged without reference to the appropriateness of use. Analysis of nursing home placement data revealed that overplacement of patients in skilled nursing facilities decreased as bed supply increased. These results suggest that the Roemer-Feldstein hypothesis may provide an inadequate rationale for regulation of nursing home bed supply. Further developments should be based on more sophisticated notions of bed supply, should make reference to the appropriateness of services, and should be subjected to empirical verification.", "PMID": 972563}
{"id": "pubmed23n0032_9034", "title": "Inpatient hospital utilization in three prepaid comprehensive health care plans compared with a regular Blue Cross plan.", "content": "The evidence is substantial that comprehensive, HMO-type prepayment plans can significiantly reduce hospitalization rates. Yet it remains unclear which factors contribute to this phenomenon. This study focuses on organizational characteristics of four plans with different hospitalization experiences. Regular medical staff review and frequent use of second opinions and economies of scale achieved by providing care at one well-equipped, large health center appear to have the largest impact. However, such organizational advantages can easily be wiped out by adverse self-selection of patients during open-enrollment periods. Evidence of selection based on predictable high obstetrical and newborn care costs is presented.", "contents": "Inpatient hospital utilization in three prepaid comprehensive health care plans compared with a regular Blue Cross plan. The evidence is substantial that comprehensive, HMO-type prepayment plans can significiantly reduce hospitalization rates. Yet it remains unclear which factors contribute to this phenomenon. This study focuses on organizational characteristics of four plans with different hospitalization experiences. Regular medical staff review and frequent use of second opinions and economies of scale achieved by providing care at one well-equipped, large health center appear to have the largest impact. However, such organizational advantages can easily be wiped out by adverse self-selection of patients during open-enrollment periods. Evidence of selection based on predictable high obstetrical and newborn care costs is presented.", "PMID": 972565}
{"id": "pubmed23n0032_9035", "title": "Factors determining the quality of physician performance in patient care.", "content": "The study determined the separate and joint effects of 1) physician specialty, 2) type of medical school attended, 3) time in practice, 4) type of ambulatory care setting, and 5) type of hospital on the quality of physician performance in patient care. The sample consisted of 454 physicians in 18 specialty categories. Multiple Classification Analysis was used to determine the effects of these predictors on the quality of physician performance. The study found that the organization of hospital care had a large independent effect, but that the other four predictors had relatively small effects on the quality of physician performance. The findings imply that the organization of the setting in which care is provided has more influence than the education and training that physicians had attained. All five predictors together explained 20 per cent of the variance. Methodological suggestions were made to improve the explanatory power of the variables, and various pragmatic for medical care administrators were presented.", "contents": "Factors determining the quality of physician performance in patient care. The study determined the separate and joint effects of 1) physician specialty, 2) type of medical school attended, 3) time in practice, 4) type of ambulatory care setting, and 5) type of hospital on the quality of physician performance in patient care. The sample consisted of 454 physicians in 18 specialty categories. Multiple Classification Analysis was used to determine the effects of these predictors on the quality of physician performance. The study found that the organization of hospital care had a large independent effect, but that the other four predictors had relatively small effects on the quality of physician performance. The findings imply that the organization of the setting in which care is provided has more influence than the education and training that physicians had attained. All five predictors together explained 20 per cent of the variance. Methodological suggestions were made to improve the explanatory power of the variables, and various pragmatic for medical care administrators were presented.", "PMID": 972566}
{"id": "pubmed23n0032_9036", "title": "Correlates of outcomes in patients with congestive heart failure.", "content": "This study relates components of care, including patient characteristics, degree of initial morbidity, and process of care, to patient outcomes. One hundred twenty-two adults who were being treated for congestive heart failure by a sample of physicians in Fort Wayne, Indiana were studied. Data were obtained on the patients' personal characteristics; degree of illness at the beginning of the six-month study period; process measures (physician awareness, communication, medication use, therapeutic management, patient satisfaction, and utilization of services); and level of activity and symptoms at the end of the study period. These components were quantified and subjected to correlation and regression analysis. The largest and most significant predictors of outcome status were measures of initial disease status. Process variables were strongly and significantly associated with outcome only in a group of patients who were minimally symptomatic initially. The lack of an overall association between process of medical care and patient outcome cannot be generalized beyond these patients with congestive heart failure. A similar approach of dissection, quantification, and analysis of components of care, however, can be used to explore a possible relationship in other conditions and care delivery settings.", "contents": "Correlates of outcomes in patients with congestive heart failure. This study relates components of care, including patient characteristics, degree of initial morbidity, and process of care, to patient outcomes. One hundred twenty-two adults who were being treated for congestive heart failure by a sample of physicians in Fort Wayne, Indiana were studied. Data were obtained on the patients' personal characteristics; degree of illness at the beginning of the six-month study period; process measures (physician awareness, communication, medication use, therapeutic management, patient satisfaction, and utilization of services); and level of activity and symptoms at the end of the study period. These components were quantified and subjected to correlation and regression analysis. The largest and most significant predictors of outcome status were measures of initial disease status. Process variables were strongly and significantly associated with outcome only in a group of patients who were minimally symptomatic initially. The lack of an overall association between process of medical care and patient outcome cannot be generalized beyond these patients with congestive heart failure. A similar approach of dissection, quantification, and analysis of components of care, however, can be used to explore a possible relationship in other conditions and care delivery settings.", "PMID": 972567}
{"id": "pubmed23n0032_9037", "title": "[Catheterization of the umbilical vein, diagnostic and therapeutic relevance (author's transl)].", "content": "Catheterization of the umbilical vein does allow direct access to the portal system, thus yielding information about the pathophysiology of the hepatic and splanchnic vessel system. Mesurement of the portal vein pressure in conditions of portal hypertension can be performed in the portal vein. A special, three-compartment ballon catheter is available for mesurement of intrahepatic wedge pressure. Angiography of the umbilical vein allows earlier diagnosis of metastases within the liver than other diagnostic procedures. Intraportal application of cytostatic drugs using a long term indwelling umbilical vein catheter is feasible as well. Own experiences are as yet rather limited. Three patients have been treated up to now this way; evaluation of the therapeutic results is not yet possible, because of the short duration of therapy and because of the low number of patients. The technique of umbilical vein catheterization does not seem to present any problems. Complications are rare and did not occur in our series.", "contents": "[Catheterization of the umbilical vein, diagnostic and therapeutic relevance (author's transl)]. Catheterization of the umbilical vein does allow direct access to the portal system, thus yielding information about the pathophysiology of the hepatic and splanchnic vessel system. Mesurement of the portal vein pressure in conditions of portal hypertension can be performed in the portal vein. A special, three-compartment ballon catheter is available for mesurement of intrahepatic wedge pressure. Angiography of the umbilical vein allows earlier diagnosis of metastases within the liver than other diagnostic procedures. Intraportal application of cytostatic drugs using a long term indwelling umbilical vein catheter is feasible as well. Own experiences are as yet rather limited. Three patients have been treated up to now this way; evaluation of the therapeutic results is not yet possible, because of the short duration of therapy and because of the low number of patients. The technique of umbilical vein catheterization does not seem to present any problems. Complications are rare and did not occur in our series.", "PMID": 972568}
{"id": "pubmed23n0032_9038", "title": "[The diameter of the pancreatic duct in relation to the size of the organ in chronic pancreatitis (author's transl)].", "content": "In 108 cases a postmortal pancreatography followed by histological examination was performed. The size of the pancreas could be measured on X-ray films after complete acinar filling. In normal cases organ size was proportional to the diameter of the duct, the diameter ranging between 2 and 4 mm (average 3,7 mm). In subjects with chronic pancreatitis, the diameter varied from 4 to 8 mm (average 5.7 mm). From these results it could be concluded, that the S/D-quotient (pancreatic surface seen on X-ray in cm2 divided by the greatest diameter of the main duct in mm) might be an aid in establishing the diagnosis of chronic pancreatitis in doubtful cases. In chronic pancreatitis the width of the duct generally increases while the size of the organ decreases due to scar formation, thereby clearly reducing the S/D-quotient. The average quotient of our normal cases was 24,2 and that for cases of chronic pancreatitis was 12,0.", "contents": "[The diameter of the pancreatic duct in relation to the size of the organ in chronic pancreatitis (author's transl)]. In 108 cases a postmortal pancreatography followed by histological examination was performed. The size of the pancreas could be measured on X-ray films after complete acinar filling. In normal cases organ size was proportional to the diameter of the duct, the diameter ranging between 2 and 4 mm (average 3,7 mm). In subjects with chronic pancreatitis, the diameter varied from 4 to 8 mm (average 5.7 mm). From these results it could be concluded, that the S/D-quotient (pancreatic surface seen on X-ray in cm2 divided by the greatest diameter of the main duct in mm) might be an aid in establishing the diagnosis of chronic pancreatitis in doubtful cases. In chronic pancreatitis the width of the duct generally increases while the size of the organ decreases due to scar formation, thereby clearly reducing the S/D-quotient. The average quotient of our normal cases was 24,2 and that for cases of chronic pancreatitis was 12,0.", "PMID": 972569}
{"id": "pubmed23n0032_9039", "title": "[Unusual lethal outcome of percutaneous needle biopsy of the liver (author's transl)].", "content": "In a 71 year old patient liver needle biopsy caused death 18 days after biopsy was done, resulting from hemorrhage into the intra- and extrahepatic bile ducts. The biopsy had caused an acute exacerbation of a clinically latent cholangitis, followed by acute purulent pylephlebitis of intrahepatic branches with vascular arrosion. Data in the literature concerning complications after percutaneous or peritoneoscopic biopsy of the liver are quoted. The causes leading to the lethal outcome are discussed.", "contents": "[Unusual lethal outcome of percutaneous needle biopsy of the liver (author's transl)]. In a 71 year old patient liver needle biopsy caused death 18 days after biopsy was done, resulting from hemorrhage into the intra- and extrahepatic bile ducts. The biopsy had caused an acute exacerbation of a clinically latent cholangitis, followed by acute purulent pylephlebitis of intrahepatic branches with vascular arrosion. Data in the literature concerning complications after percutaneous or peritoneoscopic biopsy of the liver are quoted. The causes leading to the lethal outcome are discussed.", "PMID": 972570}
{"id": "pubmed23n0032_9040", "title": "[Perforation of the esophagus by gastric tubes (author's transl)].", "content": "Esophageal lesions caused by gastric tubes are not rare nowadays. Perforation at the physiologically narrow sites of the esophagus is a wellknown mechanism as is the use of inflexible polyethylen tubes containing a mandrin. Within 12 months perforation of the esophagus caused by gastric probes occurred in four patients of our intensive care unit. Clinical symptoms, diagnostic procedures and therapy of this complication are discussed, especially emphysizing the need of early diagnosis. The extent of complications arising from and the process of healing of this iatrogenic lesions depend upon the localization of the lesion and ypon early diagnosis.", "contents": "[Perforation of the esophagus by gastric tubes (author's transl)]. Esophageal lesions caused by gastric tubes are not rare nowadays. Perforation at the physiologically narrow sites of the esophagus is a wellknown mechanism as is the use of inflexible polyethylen tubes containing a mandrin. Within 12 months perforation of the esophagus caused by gastric probes occurred in four patients of our intensive care unit. Clinical symptoms, diagnostic procedures and therapy of this complication are discussed, especially emphysizing the need of early diagnosis. The extent of complications arising from and the process of healing of this iatrogenic lesions depend upon the localization of the lesion and ypon early diagnosis.", "PMID": 972571}
{"id": "pubmed23n0032_9041", "title": "[Selection of patients with gallstones for litholysis (author's transl)].", "content": "Drug therapy of cholelithiasis with chenodeoxycloic acid (CDC) will yield better results, if patients with new cholesterol stones are selected for therapy, since these stones dissolve quickly and completely. X-ray diagnosis of the different types of stones is discussed; radiolucent bilirubin stones with or without cholesterol covering and with low calcium content do present the most difficult diagnostic problems. Old cholesterol stones do not disolve completely. If litholysis is supposed to be succesful, the diameter of a solitary cholesterol stone should not be greater than 20 mm and the total volume of cholesterol stones should not occupy more than 50% of the gallbladder volume in order to allow sufficient contact of the stones with the bile. Litholysis may be stopped if calcium salts or pigments cover the surgace of the stone. At the present stage only 24% of all patients with gallstones are suited for CDC-therapy. Early diagnosis of cholelithiasis is essential, if CDC-therapy is to be applied more frequently and if this form of therapy is to have better effects.", "contents": "[Selection of patients with gallstones for litholysis (author's transl)]. Drug therapy of cholelithiasis with chenodeoxycloic acid (CDC) will yield better results, if patients with new cholesterol stones are selected for therapy, since these stones dissolve quickly and completely. X-ray diagnosis of the different types of stones is discussed; radiolucent bilirubin stones with or without cholesterol covering and with low calcium content do present the most difficult diagnostic problems. Old cholesterol stones do not disolve completely. If litholysis is supposed to be succesful, the diameter of a solitary cholesterol stone should not be greater than 20 mm and the total volume of cholesterol stones should not occupy more than 50% of the gallbladder volume in order to allow sufficient contact of the stones with the bile. Litholysis may be stopped if calcium salts or pigments cover the surgace of the stone. At the present stage only 24% of all patients with gallstones are suited for CDC-therapy. Early diagnosis of cholelithiasis is essential, if CDC-therapy is to be applied more frequently and if this form of therapy is to have better effects.", "PMID": 972572}
{"id": "pubmed23n0032_9042", "title": "[Antegrade visualization of the billiary duct system by percutaneous transjugular cholangiography (author's transl)].", "content": "Percutaneous transjugular cholangiography (PTJC) is a new technique for visualization of the biliary duct system and for diagnosis of obstructive jaundice. First experiences of the authors in 22 patients are described. The advantages and disadvantages of this procedure, which at the present time is used rather rarely, are discussed and compared to various other cholangiographic methods. The endoscopic retrograde cholangiography (ERC) was used for patients with obstructive jaundice of unknown origine in the first place. If the biliary duct system could not be visualized by ERC (failure of complete obstruction of the common bile duct), the antegrade technique (PTJC) was performed. The peritoneoscopic and the transjugular cholangiography yield about the same percentage of positive results (90%), as far as visualization of the biliary tree is concerned. However in contrast to the peritoneoscopic methods PTJC seems to bear a smaller risk of complications. Some further diagnostic and therapeutical advantages, which might result from the use of PTJC are pointed out.", "contents": "[Antegrade visualization of the billiary duct system by percutaneous transjugular cholangiography (author's transl)]. Percutaneous transjugular cholangiography (PTJC) is a new technique for visualization of the biliary duct system and for diagnosis of obstructive jaundice. First experiences of the authors in 22 patients are described. The advantages and disadvantages of this procedure, which at the present time is used rather rarely, are discussed and compared to various other cholangiographic methods. The endoscopic retrograde cholangiography (ERC) was used for patients with obstructive jaundice of unknown origine in the first place. If the biliary duct system could not be visualized by ERC (failure of complete obstruction of the common bile duct), the antegrade technique (PTJC) was performed. The peritoneoscopic and the transjugular cholangiography yield about the same percentage of positive results (90%), as far as visualization of the biliary tree is concerned. However in contrast to the peritoneoscopic methods PTJC seems to bear a smaller risk of complications. Some further diagnostic and therapeutical advantages, which might result from the use of PTJC are pointed out.", "PMID": 972574}
{"id": "pubmed23n0032_9043", "title": "Identification of plant sterols in plasma and red blood cells of man and experimental animals.", "content": "Direct gas liquid chromatography (GLC) of total plasma lipids showed small peaks (0.5-1.5% of total free sterol area) corresponding to free C28 and C29 sterols in ca. 50% of some 3,000 normal subjects and patients with hyperlipemia. Comparable proportions of similar peaks were present in the sterol fraction isolated from the red blood cells of many of these subjects. The maximum levels of these components in the plasma and red blood cells of domestic and laboratory animals were up to 10 times higher than those seen in man. Detailed gas chromatography/mass spectrometry analyses of the plasma lipids from a much more limited number of subjects and animals showed that the GLC peaks corresponding to the free C28 and C29 sterols were largely due to the plant sterols campesterol, stigmasterol, and beta-sitosterol. In all instances, variable amounts (0.05-0.2% of the total free sterol area) of 7-dehydrocholesterol, desmosterol, lanosterol, and cholesterol alpha-oxide were also detected. While the total content and composition of the plasma plant sterols appeared to vary greatly among the subjects, it never exceeded 2% of total sterol in the normal subjects and patients examined. There was no evidence for a significant increase in the plant sterol content of the plasma of patients with hypercholesterolemia or hypertriglyceridemia.", "contents": "Identification of plant sterols in plasma and red blood cells of man and experimental animals. Direct gas liquid chromatography (GLC) of total plasma lipids showed small peaks (0.5-1.5% of total free sterol area) corresponding to free C28 and C29 sterols in ca. 50% of some 3,000 normal subjects and patients with hyperlipemia. Comparable proportions of similar peaks were present in the sterol fraction isolated from the red blood cells of many of these subjects. The maximum levels of these components in the plasma and red blood cells of domestic and laboratory animals were up to 10 times higher than those seen in man. Detailed gas chromatography/mass spectrometry analyses of the plasma lipids from a much more limited number of subjects and animals showed that the GLC peaks corresponding to the free C28 and C29 sterols were largely due to the plant sterols campesterol, stigmasterol, and beta-sitosterol. In all instances, variable amounts (0.05-0.2% of the total free sterol area) of 7-dehydrocholesterol, desmosterol, lanosterol, and cholesterol alpha-oxide were also detected. While the total content and composition of the plasma plant sterols appeared to vary greatly among the subjects, it never exceeded 2% of total sterol in the normal subjects and patients examined. There was no evidence for a significant increase in the plant sterol content of the plasma of patients with hypercholesterolemia or hypertriglyceridemia.", "PMID": 972575}
{"id": "pubmed23n0032_9044", "title": "Plasma transport forms of ingested fatty alcohols in the rat.", "content": "Previous studies have shown that ingested fatty alcohols are absorbed as fatty acids and fatty acid esters, particularly triglycerides. The present study was carried out to determine whether fatty alcohols are also transported as 0-alkyl glyceryl ethers, alk-1-enyl glyceryl ethers, and as wax esters. Oxidation of fatty alcohols to other lipids was assessed by using a mixture of [1-3H] hexadecanol and [1-14C] hexadecanol of predetermined ratio. The results indicate that the absorption of fatty alcohol, and of its transport forms, parallels the absorption of labeled fatty acids. Six to 25% of plasma radioactivity was present as 1-0-alkyl diacylglyceryl ethers with a smaller proportion of ether lipids in the phospholipid fraction. In addition, 4-13% of the ingested hexadecanol appeared in the plasma as a material having the chromatographic properties of wax ester. Fatty alcohols were not detected in the plasma as alk-1-enyl lipids.", "contents": "Plasma transport forms of ingested fatty alcohols in the rat. Previous studies have shown that ingested fatty alcohols are absorbed as fatty acids and fatty acid esters, particularly triglycerides. The present study was carried out to determine whether fatty alcohols are also transported as 0-alkyl glyceryl ethers, alk-1-enyl glyceryl ethers, and as wax esters. Oxidation of fatty alcohols to other lipids was assessed by using a mixture of [1-3H] hexadecanol and [1-14C] hexadecanol of predetermined ratio. The results indicate that the absorption of fatty alcohol, and of its transport forms, parallels the absorption of labeled fatty acids. Six to 25% of plasma radioactivity was present as 1-0-alkyl diacylglyceryl ethers with a smaller proportion of ether lipids in the phospholipid fraction. In addition, 4-13% of the ingested hexadecanol appeared in the plasma as a material having the chromatographic properties of wax ester. Fatty alcohols were not detected in the plasma as alk-1-enyl lipids.", "PMID": 972576}
{"id": "pubmed23n0032_9045", "title": "Syntheses of tetra- and hexadeuterated octadecenoates.", "content": "A study of the metabolism in man of cis and trans monoenoic acids required the synthesis of tetra- and hexadeutero-9-octadecenoates. Preparation of methyl 9-octadecenoate-8,8,11,11-d4 (90 mol % deuterium) by the Wittig reaction was accompanied by deuterium scattering with sodium methoxide as the base but not with alkylithium. Scattering occurred in both the aldehyde and phosphorane moieties only when the aldehyde contained deuterium on the alpha carbon. Methyl 9-octadecenoate-8,8,13,13,14-14-d6 (98 mol % deuterium) was also prepared by the Wittig reaction. The deuterated octadecenoates were formed principally as cis isomers. The trans isomers were produced by nitrogen oxide isomerization and separation on a silver ion column.", "contents": "Syntheses of tetra- and hexadeuterated octadecenoates. A study of the metabolism in man of cis and trans monoenoic acids required the synthesis of tetra- and hexadeutero-9-octadecenoates. Preparation of methyl 9-octadecenoate-8,8,11,11-d4 (90 mol % deuterium) by the Wittig reaction was accompanied by deuterium scattering with sodium methoxide as the base but not with alkylithium. Scattering occurred in both the aldehyde and phosphorane moieties only when the aldehyde contained deuterium on the alpha carbon. Methyl 9-octadecenoate-8,8,13,13,14-14-d6 (98 mol % deuterium) was also prepared by the Wittig reaction. The deuterated octadecenoates were formed principally as cis isomers. The trans isomers were produced by nitrogen oxide isomerization and separation on a silver ion column.", "PMID": 972577}
{"id": "pubmed23n0032_9046", "title": "Mass spectra of acetylenic fatty acid methyl esters and derivatives.", "content": "A series of isomeric methyl octadecynoates was analyzed by mass spectrometry; each isomer gave a unique spectrum. The characteristic ions were those resulting from a McLafferty rearrangement of the allenic sites or of the already-rearranged allenic sites. The acetylenic esters were also subjected to oxymercuration whereupon a carbonyl group was formed at either of the original actylenic carbon atoms providing two oxostearates. Further reaction with NaBH4 formed hydroxy esters which, after silylation, gave diagnostic mass spectra indicative of the triple bond location. Applied to esters with both double and triple bonds, this procedure permitted differentiation between the two types of unsaturation. Methoxyl groups marked the original double bond locations and hydroxyls did so for triple bonds.", "contents": "Mass spectra of acetylenic fatty acid methyl esters and derivatives. A series of isomeric methyl octadecynoates was analyzed by mass spectrometry; each isomer gave a unique spectrum. The characteristic ions were those resulting from a McLafferty rearrangement of the allenic sites or of the already-rearranged allenic sites. The acetylenic esters were also subjected to oxymercuration whereupon a carbonyl group was formed at either of the original actylenic carbon atoms providing two oxostearates. Further reaction with NaBH4 formed hydroxy esters which, after silylation, gave diagnostic mass spectra indicative of the triple bond location. Applied to esters with both double and triple bonds, this procedure permitted differentiation between the two types of unsaturation. Methoxyl groups marked the original double bond locations and hydroxyls did so for triple bonds.", "PMID": 972578}
{"id": "pubmed23n0032_9047", "title": "Kinetics of absorption, equilibration (or distribution), and excretion of orally and intraperitoneally administered cholesterol in the rat.", "content": "Resorption and metabolism of cholesterol administered intraperitoneally and per os have been described in the rat utilizing a technique of double isotopic labeling. Depending upon the route of administration, these parameter vary to a large degree. Alimentary cholesterol is progressively resorbed over a period of 10 hr, while the maximal blood level of cholesterol is attained after the 6th hr following intraperitoneal injection. On the other hand, cholesterol administered per os is more rapidly utilized in biliary acid synthesis than cholesterol administered intraperitoneally. In the range of concentrations utilized in this work (10-300 mug and 0.2-300 mug, respectively, administered to the rat, orally and intraperitoneally), the rate of cholesterol resorption remained constant.", "contents": "Kinetics of absorption, equilibration (or distribution), and excretion of orally and intraperitoneally administered cholesterol in the rat. Resorption and metabolism of cholesterol administered intraperitoneally and per os have been described in the rat utilizing a technique of double isotopic labeling. Depending upon the route of administration, these parameter vary to a large degree. Alimentary cholesterol is progressively resorbed over a period of 10 hr, while the maximal blood level of cholesterol is attained after the 6th hr following intraperitoneal injection. On the other hand, cholesterol administered per os is more rapidly utilized in biliary acid synthesis than cholesterol administered intraperitoneally. In the range of concentrations utilized in this work (10-300 mug and 0.2-300 mug, respectively, administered to the rat, orally and intraperitoneally), the rate of cholesterol resorption remained constant.", "PMID": 972579}
{"id": "pubmed23n0032_9048", "title": "Age-related changes in the lipid metabolism of Fisher 344 rats.", "content": "Lipid metabolism of male Fisher 344 rats aged 2-24 months was studied. Serum and liver cholesterol levels did not display the age-related gradual increase seen in other rat strains. An increase in the serum plus liver cholesterol pool from 2 to 6 months was followed by a plateau through 18 months and then another increase at 24 months of age. The triglyceride pool increased from 2 to 6 months and then remained unchanged through 24 months of age. Cholesterol synthesis from acetate decreased 50% between 2 and 9 months and fell only slightly through 24 months of age. Assay of 3-hydroxy-3-methyl glutaryl Coenzyme A (HMG-CoA) reductase showed a similar pattern but did not decrease further after 9 months of age. Cholesterol 7alpha hydroxylase activity was not significantly altered by age. These age- and strain-related differences present an opportunity for a comparative study of the aging process using the parameters of lipid metabolism as indicators.", "contents": "Age-related changes in the lipid metabolism of Fisher 344 rats. Lipid metabolism of male Fisher 344 rats aged 2-24 months was studied. Serum and liver cholesterol levels did not display the age-related gradual increase seen in other rat strains. An increase in the serum plus liver cholesterol pool from 2 to 6 months was followed by a plateau through 18 months and then another increase at 24 months of age. The triglyceride pool increased from 2 to 6 months and then remained unchanged through 24 months of age. Cholesterol synthesis from acetate decreased 50% between 2 and 9 months and fell only slightly through 24 months of age. Assay of 3-hydroxy-3-methyl glutaryl Coenzyme A (HMG-CoA) reductase showed a similar pattern but did not decrease further after 9 months of age. Cholesterol 7alpha hydroxylase activity was not significantly altered by age. These age- and strain-related differences present an opportunity for a comparative study of the aging process using the parameters of lipid metabolism as indicators.", "PMID": 972580}
{"id": "pubmed23n0032_9049", "title": "Fecal long chain fatty acids and colon cancer risk.", "content": "To determine whether excretion of high concentrations of long chain fatty acids might be associated with high colon cancer risk, we compared concentrations of major long chain fatty acids in the feces of four populations at different risk for colon cancer. Concentrations of C18:1 were found to be significantly higher (P less than 0.05) in the feces of the two high risk populations than in the feces of the two low risk populations.", "contents": "Fecal long chain fatty acids and colon cancer risk. To determine whether excretion of high concentrations of long chain fatty acids might be associated with high colon cancer risk, we compared concentrations of major long chain fatty acids in the feces of four populations at different risk for colon cancer. Concentrations of C18:1 were found to be significantly higher (P less than 0.05) in the feces of the two high risk populations than in the feces of the two low risk populations.", "PMID": 972581}
{"id": "pubmed23n0032_9050", "title": "Object analysis of the Stilling tables.", "content": "The CIE space that is occupied by the Stilling tables was found to be similar to that occupied by the Ishihara plates [LAKOWSKI, 1965] with the notable exceptions of a single table that extends into the blue area and two others that extend further into the green (fig. 4). Spectrophotometric curves show that the inks used are similar to those in other PIC plates, i.e. they have reflectance curves with broad-band characteristics. All tables are of the vanishing type and there is an attempt to differentiate between protan and deutan defects. Colorimetrically, tables I, II, VII and VIII have figure and background colours aligned mainly along the deutan isochromatic lines and only table III is a clear protan plate. Tables V, VI, IX, X and XI are ambiguous in design, i.e. they would be confused by most colour-deficient persons. Of the tables designed to detect blue-yellow deficiencies (tables V, VI, XI and XII), only the colours in table XII are aligned along tetartan confusion lines, while the other three tables are indefinite in their design. Thus the usefulness of these plates for detecting acquired dyschromatopsias is limited.", "contents": "Object analysis of the Stilling tables. The CIE space that is occupied by the Stilling tables was found to be similar to that occupied by the Ishihara plates [LAKOWSKI, 1965] with the notable exceptions of a single table that extends into the blue area and two others that extend further into the green (fig. 4). Spectrophotometric curves show that the inks used are similar to those in other PIC plates, i.e. they have reflectance curves with broad-band characteristics. All tables are of the vanishing type and there is an attempt to differentiate between protan and deutan defects. Colorimetrically, tables I, II, VII and VIII have figure and background colours aligned mainly along the deutan isochromatic lines and only table III is a clear protan plate. Tables V, VI, IX, X and XI are ambiguous in design, i.e. they would be confused by most colour-deficient persons. Of the tables designed to detect blue-yellow deficiencies (tables V, VI, XI and XII), only the colours in table XII are aligned along tetartan confusion lines, while the other three tables are indefinite in their design. Thus the usefulness of these plates for detecting acquired dyschromatopsias is limited.", "PMID": 972605}
{"id": "pubmed23n0032_9051", "title": "Examination of chromatic adaptation.", "content": "The author describes a simple self-constructed apparatus which is suitable to perform investigations on adaptation in four domain of the spectrum. He considers the apparatus as appropriate to examine acquired colour vision deficiencies.", "contents": "Examination of chromatic adaptation. The author describes a simple self-constructed apparatus which is suitable to perform investigations on adaptation in four domain of the spectrum. He considers the apparatus as appropriate to examine acquired colour vision deficiencies.", "PMID": 972610}
{"id": "pubmed23n0032_9052", "title": "Color vision and visually evoked response (VECP) in the recovery period of optic neuritis.", "content": "Color vision and visually evoked cortical potentials (VECPs) are compared in a group of patients with optic neuritis. In the early recovery period, both were disturbed in a similar degree, whereas in the late recovery period, the VECPs remained more affected than color vision.", "contents": "Color vision and visually evoked response (VECP) in the recovery period of optic neuritis. Color vision and visually evoked cortical potentials (VECPs) are compared in a group of patients with optic neuritis. In the early recovery period, both were disturbed in a similar degree, whereas in the late recovery period, the VECPs remained more affected than color vision.", "PMID": 972611}
{"id": "pubmed23n0032_9053", "title": "Chromosome aberrations in oocytes of NMRI mice and bone marrow cells of Chinese hamsters induced with 3,4-benzpyrene.", "content": "This paper deals with mutagenicity tests with 3,4-benzpyrene in mice oocytes of the strain NMRI and Chinese hamster bone-marrow cells. The mutagenic effect of this hydrocarbon was observed in both test systems. Investigations of bone-marrow chromosomes clearly show dose-response curves.", "contents": "Chromosome aberrations in oocytes of NMRI mice and bone marrow cells of Chinese hamsters induced with 3,4-benzpyrene. This paper deals with mutagenicity tests with 3,4-benzpyrene in mice oocytes of the strain NMRI and Chinese hamster bone-marrow cells. The mutagenic effect of this hydrocarbon was observed in both test systems. Investigations of bone-marrow chromosomes clearly show dose-response curves.", "PMID": 972619}
{"id": "pubmed23n0032_9054", "title": "Injuries to research subjects A survey of investigators.", "content": "A task force of the Department of Health, Education, and Welfare conducted a survey aimed at estimating the incidence of research-related injuries, with a view to determining the feasibility of compensating subjects injured during research. The data were obtained by telephone from 331 investigators conducting research on nearly 133,000 human subjects over the past three years. Eighty-five investigators reported at least one injury. Of the 4957 reported injuries, 3926 were classified as trivial, and 974 as temporarily disabling; of 57 injuries resulting in death or permanent disability, one disabling stroke, not clearly related to the research, occurred three days after a non-therapeutic procedure; the rest resulted from treatments expected to benefit the patients directly, usually cancer chemotherapy. The data suggest that the risks of participation in nontherapeutic research may be of no greater than those of everyday life, and in therapeutic research, no greater than those of treatment in other settings.", "contents": "Injuries to research subjects A survey of investigators. A task force of the Department of Health, Education, and Welfare conducted a survey aimed at estimating the incidence of research-related injuries, with a view to determining the feasibility of compensating subjects injured during research. The data were obtained by telephone from 331 investigators conducting research on nearly 133,000 human subjects over the past three years. Eighty-five investigators reported at least one injury. Of the 4957 reported injuries, 3926 were classified as trivial, and 974 as temporarily disabling; of 57 injuries resulting in death or permanent disability, one disabling stroke, not clearly related to the research, occurred three days after a non-therapeutic procedure; the rest resulted from treatments expected to benefit the patients directly, usually cancer chemotherapy. The data suggest that the risks of participation in nontherapeutic research may be of no greater than those of everyday life, and in therapeutic research, no greater than those of treatment in other settings.", "PMID": 972643}
{"id": "pubmed23n0032_9055", "title": "Predictors of epilepsy in children who have experienced febrile seizures.", "content": "We examined the frequency of development of afebrile seizures in 1706 children who had experienced at least one febrile seizure and were followed to the age of seven years. Epilepsy developed by seven years of age in 20 per 1000 (2 per cent), and another 10 per 1000 had at least one afebrile seizure that did not meet our definition of epilepsy. In children whose neurologic or developmental status was suspect or abnormal before any seizure and whose first seizure was complex (longer than 15 minutes, multiple or focal) epilepsy developed at a rate 18 times higher than in children with no febrile seizures (92 vs. 5 per 1000; P less than 0.001). In the largest group with febrile seizures, those previously normal with noncomplex first febrile seizures, epilepsy developed in 11 per 1000; this rate, although moderate, was greater than that for children with no febrile seizures (P = 0.027). Prior neurologic and developmental status and characteristics of the first febrile seizure are important predictors of epilepsy after febrile seizures.", "contents": "Predictors of epilepsy in children who have experienced febrile seizures. We examined the frequency of development of afebrile seizures in 1706 children who had experienced at least one febrile seizure and were followed to the age of seven years. Epilepsy developed by seven years of age in 20 per 1000 (2 per cent), and another 10 per 1000 had at least one afebrile seizure that did not meet our definition of epilepsy. In children whose neurologic or developmental status was suspect or abnormal before any seizure and whose first seizure was complex (longer than 15 minutes, multiple or focal) epilepsy developed at a rate 18 times higher than in children with no febrile seizures (92 vs. 5 per 1000; P less than 0.001). In the largest group with febrile seizures, those previously normal with noncomplex first febrile seizures, epilepsy developed in 11 per 1000; this rate, although moderate, was greater than that for children with no febrile seizures (P = 0.027). Prior neurologic and developmental status and characteristics of the first febrile seizure are important predictors of epilepsy after febrile seizures.", "PMID": 972656}
{"id": "pubmed23n0032_9056", "title": "Decreased bioavailability of digoxin due to antacids and kaolin-pectin.", "content": "Employing a Latin-square design and single-dose studies of bioavailability in 10 normal human volunteers, we tested the hypothesis that antacids and kaolin-pectin might interfere with the bioavailability of orally administered digoxin. Cumulative six-day urinary digoxin excretion (expressed as the percentage of a 0.75-mg dose recovered) was: control, 40.1+/-3.0 (S.E.); aluminum hydroxide, 30.7+/-2.9; magnesium hydroxide, 27.1+/-2.4; magnesium trisilicate, 29.1+/-1.7; and kaolin-pectin 23.4+/-2.0. The differences in means were highly significant (F = 10.47, P less than 0.005). Further analysis (multiple comparison test) revealed that control differed significantly from each of the other treatments (alpha = 0.05), but there was no such difference between any of the other treatment groups. The decreased cumulative excretion produced by antacids and kaolin-pectin reflected a striking reduction in digoxin absorption associated with these compounds that was not related to alteration of gut transit time or to adsorption of digoxin to these gastrointestinal medications.", "contents": "Decreased bioavailability of digoxin due to antacids and kaolin-pectin. Employing a Latin-square design and single-dose studies of bioavailability in 10 normal human volunteers, we tested the hypothesis that antacids and kaolin-pectin might interfere with the bioavailability of orally administered digoxin. Cumulative six-day urinary digoxin excretion (expressed as the percentage of a 0.75-mg dose recovered) was: control, 40.1+/-3.0 (S.E.); aluminum hydroxide, 30.7+/-2.9; magnesium hydroxide, 27.1+/-2.4; magnesium trisilicate, 29.1+/-1.7; and kaolin-pectin 23.4+/-2.0. The differences in means were highly significant (F = 10.47, P less than 0.005). Further analysis (multiple comparison test) revealed that control differed significantly from each of the other treatments (alpha = 0.05), but there was no such difference between any of the other treatment groups. The decreased cumulative excretion produced by antacids and kaolin-pectin reflected a striking reduction in digoxin absorption associated with these compounds that was not related to alteration of gut transit time or to adsorption of digoxin to these gastrointestinal medications.", "PMID": 972657}
{"id": "pubmed23n0032_9057", "title": "Enzyme evolution in a microbial community growing on the herbicide Dalapon.", "content": "A seven-membered microbial community capable of utilising the herbicide Dalapon has been isolated by continuous-flow enrichment culture. The composition of this community has remained remarkably stable over thousands of hours in a Dalapon-limited chemostat. During this period, however, one member of the community, Pseudomonas putida, acquired the ability to grow on Dalapon through the evolution of an extant dehalogenase.", "contents": "Enzyme evolution in a microbial community growing on the herbicide Dalapon. A seven-membered microbial community capable of utilising the herbicide Dalapon has been isolated by continuous-flow enrichment culture. The composition of this community has remained remarkably stable over thousands of hours in a Dalapon-limited chemostat. During this period, however, one member of the community, Pseudomonas putida, acquired the ability to grow on Dalapon through the evolution of an extant dehalogenase.", "PMID": 972691}
{"id": "pubmed23n0032_9058", "title": "Partial purification of rabbit aorta contracting substance-releasing factor and inhibition of its activity by anti-inflammatory steroids.", "content": "Rabbit aorta contracting substance-releasing factor (RCS-RF) is found in perfusates from guinea pig lungs during anaphylaxis. It has been identified as a small peptide which releases arachidonic acid from lung tissue, thus generating prostaglandin endoperoxides and tbromboxanes and causing bronchocconstriction. Anti-inflammatory steroids block the release of arachidonate by RCS-RF. In this activity their relative potency is very similar to their relative anti-inflammatory potency, suggesting that the two actions may be related.", "contents": "Partial purification of rabbit aorta contracting substance-releasing factor and inhibition of its activity by anti-inflammatory steroids. Rabbit aorta contracting substance-releasing factor (RCS-RF) is found in perfusates from guinea pig lungs during anaphylaxis. It has been identified as a small peptide which releases arachidonic acid from lung tissue, thus generating prostaglandin endoperoxides and tbromboxanes and causing bronchocconstriction. Anti-inflammatory steroids block the release of arachidonate by RCS-RF. In this activity their relative potency is very similar to their relative anti-inflammatory potency, suggesting that the two actions may be related.", "PMID": 972692}
{"id": "pubmed23n0032_9059", "title": "The renin-angiotensin-aldosterone system in mother and fetus at term.", "content": "Plasma renin activity, renin substrate, angiotensin II, aldosterone and cortisol were measured concurrently and renin concentration calculated in plasma from mothers during labor and delivery, from cord and from newborn infants. The renin-angiotensin-aldosterone system was found strongly stimulated in both mother and fetus. The high values of plasma renin activity in fetus were due exclusively to the high renin concentrations the substrate concentration being normal. In the mother, however, the markedly elevated renin substrate resulted in a doubling of relative values of renin activity compared to renin concentration. Therefore gradients of renin and renin substrate across the placenta are established, but the resulting renin activity is similar on both sides and the levels of generated angiotensin II are also nearly indentical with a good correlation between these last parameters. Aldosterone is as elevated in mother as in fetus whereas cortisol, due to its binding to transcortin, is twice as high in mother as in fetus. No correlation was found between renin activity or concentration of angiotensin II and aldosterone or cortisol indicating that other factors controlling aldosterone are involved.", "contents": "The renin-angiotensin-aldosterone system in mother and fetus at term. Plasma renin activity, renin substrate, angiotensin II, aldosterone and cortisol were measured concurrently and renin concentration calculated in plasma from mothers during labor and delivery, from cord and from newborn infants. The renin-angiotensin-aldosterone system was found strongly stimulated in both mother and fetus. The high values of plasma renin activity in fetus were due exclusively to the high renin concentrations the substrate concentration being normal. In the mother, however, the markedly elevated renin substrate resulted in a doubling of relative values of renin activity compared to renin concentration. Therefore gradients of renin and renin substrate across the placenta are established, but the resulting renin activity is similar on both sides and the levels of generated angiotensin II are also nearly indentical with a good correlation between these last parameters. Aldosterone is as elevated in mother as in fetus whereas cortisol, due to its binding to transcortin, is twice as high in mother as in fetus. No correlation was found between renin activity or concentration of angiotensin II and aldosterone or cortisol indicating that other factors controlling aldosterone are involved.", "PMID": 972715}
{"id": "pubmed23n0032_9060", "title": "Ultrafiltrable uric acid in serum of healthy humans and patients with renal calcium lithiasis.", "content": "Ultrafiltrable serum uric acid (u.a.) was determined by ultrafiltration under in vivo conditions in humans using a reliable technique described in detail. It could be demonstrated that u.a. binding to macromolecules occurs in healthy humans (controls) and in patients with renal calcium stones. The percentage of free u.a. in controls (n=60) averages 86.2 +/- 0.9 SEM. With increasing age, bound u.a. rises slightly. On the other hand, younger (less than 40 years) stone patients have significantly more bound u.a. than matched controls (80.7 +/- 1.0 SEM; p less than 0.001), whereas this is not found in elderly patients. The degree of binding is not related to concentration of plasma proteins but inversely related to free fatty acid concentration in healthy controls (r= -0.52; p less than 0.01). It is suggested that no augmentation of tubular u.a. filtered by the glomeruli could have occurred. The origin of fasting hyperuricosuria shown earlier to be a prominent feature of young renal calcium stone formers is yet unknown.", "contents": "Ultrafiltrable uric acid in serum of healthy humans and patients with renal calcium lithiasis. Ultrafiltrable serum uric acid (u.a.) was determined by ultrafiltration under in vivo conditions in humans using a reliable technique described in detail. It could be demonstrated that u.a. binding to macromolecules occurs in healthy humans (controls) and in patients with renal calcium stones. The percentage of free u.a. in controls (n=60) averages 86.2 +/- 0.9 SEM. With increasing age, bound u.a. rises slightly. On the other hand, younger (less than 40 years) stone patients have significantly more bound u.a. than matched controls (80.7 +/- 1.0 SEM; p less than 0.001), whereas this is not found in elderly patients. The degree of binding is not related to concentration of plasma proteins but inversely related to free fatty acid concentration in healthy controls (r= -0.52; p less than 0.01). It is suggested that no augmentation of tubular u.a. filtered by the glomeruli could have occurred. The origin of fasting hyperuricosuria shown earlier to be a prominent feature of young renal calcium stone formers is yet unknown.", "PMID": 972716}
{"id": "pubmed23n0032_9061", "title": "Prevention of hepatitis type B with specific immune serum globulin.", "content": "In order to evaluate the preventive value of specific immune serum globulin against hepatitis type B, we have used this immune globulin in required doses in 12 patients (10 with AU antigen negative and 2 with AU antigen positive) with chronic renal failure who required maintenance hemodialysis for a period of 15 months, and we were able to prevent hepatitis type B in our dialysis patients.", "contents": "Prevention of hepatitis type B with specific immune serum globulin. In order to evaluate the preventive value of specific immune serum globulin against hepatitis type B, we have used this immune globulin in required doses in 12 patients (10 with AU antigen negative and 2 with AU antigen positive) with chronic renal failure who required maintenance hemodialysis for a period of 15 months, and we were able to prevent hepatitis type B in our dialysis patients.", "PMID": 972717}
{"id": "pubmed23n0032_9062", "title": "Alterations in the H-reflex in the paraplegic induced by bladder distention.", "content": "This study has evaluated the changes in the characteristics of the H-reflex induced by distention of the urinary bladder in patients with complete lesions of the spinal cord. These findings were compared to induced changes in the systolic blood pressure and some degree of correlation was noted. It is well recognised that distention of the urinary bladder can exert a significant influence on the state of both somatic and autonomic reflex activity in spinal man. Although the mode of action of this stimulus has not been fully delineated, it is of some clinical importance since it may intensify muscular spasticity and autonomic hyperreflexia. This study was undertaken to further explore this relationship utilising the H-wave as a measure of segmental reflex activity. When appropriate, the effect of bladder distention on arterial blood pressure was also measured and an attempt made to correlate these observations with the H-wave findings.", "contents": "Alterations in the H-reflex in the paraplegic induced by bladder distention. This study has evaluated the changes in the characteristics of the H-reflex induced by distention of the urinary bladder in patients with complete lesions of the spinal cord. These findings were compared to induced changes in the systolic blood pressure and some degree of correlation was noted. It is well recognised that distention of the urinary bladder can exert a significant influence on the state of both somatic and autonomic reflex activity in spinal man. Although the mode of action of this stimulus has not been fully delineated, it is of some clinical importance since it may intensify muscular spasticity and autonomic hyperreflexia. This study was undertaken to further explore this relationship utilising the H-wave as a measure of segmental reflex activity. When appropriate, the effect of bladder distention on arterial blood pressure was also measured and an attempt made to correlate these observations with the H-wave findings.", "PMID": 972756}
{"id": "pubmed23n0032_9063", "title": "Upper and lower motor neuron lesions in the upper extremity muscles of tetraplegics.", "content": "Paralysed upper extremity muscles of 24 tetraplegic patients were examined to determine whether their lower motor neuron was intact. Primary emphasis was placed on the forearm finger flexor muscles (Flexor Digitorum Superficialis and Flexor Digitorum Profundus) and finger extensor muscles (Extensor Digitorum Communis and Extensor Indicis). It was found that the vast majority of these muscles in C4, C5 and C6 subjects retained some or all of their lower motor neurons intact. In C5 subjects, examination was performed on additional muscles that were potentially most useful in an orthosis utilising functional stimulation. Similar to the results of the finger flexor and extensor muscles, the muscles of the wrist and thumb generally had the lower motor neuron at least partially intact. A muscle that often was an exception was M. Flexor Carpi Radialis. These studies indicate that most of the forearm muscles of the upper extremity in high level spinal cord injury patients which are paralysed have the lower motor neuron totally or partially intact. With electrically induced exercise, such muscles are strengthened to functional levels of contraction. Subsequent electrical activation enables the quadriplegic patients to use the muscle functionally.", "contents": "Upper and lower motor neuron lesions in the upper extremity muscles of tetraplegics. Paralysed upper extremity muscles of 24 tetraplegic patients were examined to determine whether their lower motor neuron was intact. Primary emphasis was placed on the forearm finger flexor muscles (Flexor Digitorum Superficialis and Flexor Digitorum Profundus) and finger extensor muscles (Extensor Digitorum Communis and Extensor Indicis). It was found that the vast majority of these muscles in C4, C5 and C6 subjects retained some or all of their lower motor neurons intact. In C5 subjects, examination was performed on additional muscles that were potentially most useful in an orthosis utilising functional stimulation. Similar to the results of the finger flexor and extensor muscles, the muscles of the wrist and thumb generally had the lower motor neuron at least partially intact. A muscle that often was an exception was M. Flexor Carpi Radialis. These studies indicate that most of the forearm muscles of the upper extremity in high level spinal cord injury patients which are paralysed have the lower motor neuron totally or partially intact. With electrically induced exercise, such muscles are strengthened to functional levels of contraction. Subsequent electrical activation enables the quadriplegic patients to use the muscle functionally.", "PMID": 972757}
{"id": "pubmed23n0032_9064", "title": "Silent hydronephrosis, a hazard revisited.", "content": "Six patients with neurogenic bladder secondary to spinal cord injury were seen in our Centre for routine follow-up. All of these individuals had attained the catheter-free state by various means and were, by their standards, functioning very well. They had gone from 6 months to 2 1/2 years without genito-urinary re-evaluations. One individual had a normal urogram 1 year after catheter removal and then 2 years later was noted to have bilateral hydronephrosis. Development of silent hydronephrosis in the catheter-free state, its treatment, and a regimen for following patients are discussed.", "contents": "Silent hydronephrosis, a hazard revisited. Six patients with neurogenic bladder secondary to spinal cord injury were seen in our Centre for routine follow-up. All of these individuals had attained the catheter-free state by various means and were, by their standards, functioning very well. They had gone from 6 months to 2 1/2 years without genito-urinary re-evaluations. One individual had a normal urogram 1 year after catheter removal and then 2 years later was noted to have bilateral hydronephrosis. Development of silent hydronephrosis in the catheter-free state, its treatment, and a regimen for following patients are discussed.", "PMID": 972759}
{"id": "pubmed23n0032_9065", "title": "Long-term follow-up of paraplegic patients with vesico-ureteric reflux.", "content": "A long-term survey (mean follow-up greater than 12 years) is presented of cord injury patients with reflux up to the kidney (grades 2 and 3). Of 39 patients 17 developed renal damage. There is no evidence that permanent urethral catheters protect kidneys from subsequent damage.", "contents": "Long-term follow-up of paraplegic patients with vesico-ureteric reflux. A long-term survey (mean follow-up greater than 12 years) is presented of cord injury patients with reflux up to the kidney (grades 2 and 3). Of 39 patients 17 developed renal damage. There is no evidence that permanent urethral catheters protect kidneys from subsequent damage.", "PMID": 972760}
{"id": "pubmed23n0032_9066", "title": "Percutaneous cystostomy in paraplegia--a follow-up of 41 patients.", "content": "Percutaneous cystostomy using a fine calibre (9 Charriere) plastic trocar and cannula has been used in 51 male patients in the early weeks following spinal cord injury. The results are satisfactory. Of the last ten patients none has been catheterised per urethram; eight of these patients are now passing urine satisfactorily using a condom urinal.", "contents": "Percutaneous cystostomy in paraplegia--a follow-up of 41 patients. Percutaneous cystostomy using a fine calibre (9 Charriere) plastic trocar and cannula has been used in 51 male patients in the early weeks following spinal cord injury. The results are satisfactory. Of the last ten patients none has been catheterised per urethram; eight of these patients are now passing urine satisfactorily using a condom urinal.", "PMID": 972761}
{"id": "pubmed23n0032_9067", "title": "Hydroelectrolytic determination in paraplegics (total body water; exchangeable sodium and total body potassium).", "content": "Measurements of total body water and total exchangeable sodium, by isotopic dilution, and of total body potassium by whole body counting of the naturally occurring 40K isotope, were performed on stabilised paraplegic or tetraplegic subjects. Nineteen patients were investigated by the complete set of tests, six by only body counting. The results of total body potassium are expressed, following a uniform scale, as \"relative values\", which were established by the processing of reference values, from numberous paired healthy subjects.", "contents": "Hydroelectrolytic determination in paraplegics (total body water; exchangeable sodium and total body potassium). Measurements of total body water and total exchangeable sodium, by isotopic dilution, and of total body potassium by whole body counting of the naturally occurring 40K isotope, were performed on stabilised paraplegic or tetraplegic subjects. Nineteen patients were investigated by the complete set of tests, six by only body counting. The results of total body potassium are expressed, following a uniform scale, as \"relative values\", which were established by the processing of reference values, from numberous paired healthy subjects.", "PMID": 972762}
{"id": "pubmed23n0032_9068", "title": "Long-term studies about orthostatic training after high spinal cord injury.", "content": "Results of long-term observations of paraplegics with upper lesions are presented. The circulatory behaviour of patients, completely bedridden for several months, was observed during orthostatic tilt-table exercise over a period of at least 7 weeks. It is not possible to achieve a decisive circulatory stabilisation in paraplegics with upper lesions. For the most part, there was no improvement in the process of adaptation to varying orthostatic intensity. Much more marked are fluctuations in the ability to adapt to orthostasis, whereby the maximum circulatory lability may not occur until several weeks after the start of therapeutic repositioning to sitting. In practice, these observations point to the need for a sufficiently long period of orthostatic observation of these patients' circulatory system.", "contents": "Long-term studies about orthostatic training after high spinal cord injury. Results of long-term observations of paraplegics with upper lesions are presented. The circulatory behaviour of patients, completely bedridden for several months, was observed during orthostatic tilt-table exercise over a period of at least 7 weeks. It is not possible to achieve a decisive circulatory stabilisation in paraplegics with upper lesions. For the most part, there was no improvement in the process of adaptation to varying orthostatic intensity. Much more marked are fluctuations in the ability to adapt to orthostasis, whereby the maximum circulatory lability may not occur until several weeks after the start of therapeutic repositioning to sitting. In practice, these observations point to the need for a sufficiently long period of orthostatic observation of these patients' circulatory system.", "PMID": 972763}
{"id": "pubmed23n0032_9069", "title": "New interpretations of lipoproteins and arterial lesions of cholesterol-fed rabbits.", "content": "The serum of cholesterol-fed rabbits contains lipoproteins rich in cholesterol, in contrast to those in thoracic duct lymph, which are rich in triglyceride. This study suggests that the serum lipoproteins may be \"remnant\" chylomicrons which have been stripped of their triglyceride, but which have not been removed from circulation by the liver, as usually occurs rapidly in other animal species. Electrophoresis of rabbit serum showed a broad beta band corresponding to the very low density lipoprotein fraction separated by ultracentritrifugation. This broad beta band may represent the \"remnant particles\". This proposed \"remnant particle\" hyperlipidaemia in rabbits may have its parallel in Type III human hyperlipidaemia, which also has a broad beta band on electrophoresis and may be a \"remnant particle\" disease. It was also found that early arterial lesions in rabbits are similar to some human fatty streaks, which if allowed to age for a further two years while the rabbits eat normal food, do not regress but mature to resemble calcified fibrous plaques.", "contents": "New interpretations of lipoproteins and arterial lesions of cholesterol-fed rabbits. The serum of cholesterol-fed rabbits contains lipoproteins rich in cholesterol, in contrast to those in thoracic duct lymph, which are rich in triglyceride. This study suggests that the serum lipoproteins may be \"remnant\" chylomicrons which have been stripped of their triglyceride, but which have not been removed from circulation by the liver, as usually occurs rapidly in other animal species. Electrophoresis of rabbit serum showed a broad beta band corresponding to the very low density lipoprotein fraction separated by ultracentritrifugation. This broad beta band may represent the \"remnant particles\". This proposed \"remnant particle\" hyperlipidaemia in rabbits may have its parallel in Type III human hyperlipidaemia, which also has a broad beta band on electrophoresis and may be a \"remnant particle\" disease. It was also found that early arterial lesions in rabbits are similar to some human fatty streaks, which if allowed to age for a further two years while the rabbits eat normal food, do not regress but mature to resemble calcified fibrous plaques.", "PMID": 972765}
{"id": "pubmed23n0032_9070", "title": "Methicillin-sensitive variants in ageing broth cultures of methicillin-resistant Staphylococcus aureus.", "content": "Methicillin-sensitive variants have been isolated from ageing broth cultures of the methicillin-resistant strain of Staphylococcus aureus NCTC 10442 and also from numerous other strains. There were wide variations and fluctuations in the incidence of sensitive variants from cultures incubated under uniform conditions.", "contents": "Methicillin-sensitive variants in ageing broth cultures of methicillin-resistant Staphylococcus aureus. Methicillin-sensitive variants have been isolated from ageing broth cultures of the methicillin-resistant strain of Staphylococcus aureus NCTC 10442 and also from numerous other strains. There were wide variations and fluctuations in the incidence of sensitive variants from cultures incubated under uniform conditions.", "PMID": 972766}
{"id": "pubmed23n0032_9071", "title": "Delayed and prolonged vascular leakage in inflammation: the effects of dehydromonocrotaline on blood vessels in the rat cremaster.", "content": "Local application of dehydromonocrotaline to the rat cremaster produces a delayed prolonged increase in vascular permeability with a time course similar to that of the pulmonary oedema seen after intravenous injection of the same substance. Study of the injured area by the carbon labelling technique and by electron microscopy shows that the increased permeability involves both capillaries and venules of all sizes within the region exposed to dehydromonocrotaline. The vascular leakage appears to be due to a direct effect on the endothelium of small blood vessels. Carbon deposition in labelled capillaries and venules is predominantly intramural and indicative of increased vascular permeability. Accumulation of carbon within the lumen of capillaries is uncommon, and accounts for only a small fraction of total capillary labelling. The findings indicate that capillaries are a major source of inflammatory exudate in this type of injury and suggest that the importance of leakage from capillaries has been underestimated in other types of delayed prolonged increase in vascular permeability.", "contents": "Delayed and prolonged vascular leakage in inflammation: the effects of dehydromonocrotaline on blood vessels in the rat cremaster. Local application of dehydromonocrotaline to the rat cremaster produces a delayed prolonged increase in vascular permeability with a time course similar to that of the pulmonary oedema seen after intravenous injection of the same substance. Study of the injured area by the carbon labelling technique and by electron microscopy shows that the increased permeability involves both capillaries and venules of all sizes within the region exposed to dehydromonocrotaline. The vascular leakage appears to be due to a direct effect on the endothelium of small blood vessels. Carbon deposition in labelled capillaries and venules is predominantly intramural and indicative of increased vascular permeability. Accumulation of carbon within the lumen of capillaries is uncommon, and accounts for only a small fraction of total capillary labelling. The findings indicate that capillaries are a major source of inflammatory exudate in this type of injury and suggest that the importance of leakage from capillaries has been underestimated in other types of delayed prolonged increase in vascular permeability.", "PMID": 972767}
{"id": "pubmed23n0032_9072", "title": "Tetracycline dependence in a strain of Staphylococcus epidermidis.", "content": "An isolate of Staphylococcus epidermidis exhibited an enhanced zone of growth around a tetracycline disc. All six tetracycline-related compounds investigated produced this effect, which could only be demonstrated on certain media. Modification of this phenomenon resulted from variation of the incubation temperature.", "contents": "Tetracycline dependence in a strain of Staphylococcus epidermidis. An isolate of Staphylococcus epidermidis exhibited an enhanced zone of growth around a tetracycline disc. All six tetracycline-related compounds investigated produced this effect, which could only be demonstrated on certain media. Modification of this phenomenon resulted from variation of the incubation temperature.", "PMID": 972768}
{"id": "pubmed23n0032_9073", "title": "Experimental autoimmune glomerulonephritis in the rat. I. Factors influencing induction.", "content": "Albino Holtzman, albino Wistar and hooded HS rats were injected fortnightly for 14 weeks with human glomerular basement membrane (GBM) emulsified in Freund's complete adjuvant. Half of the rats were pretreated with Freund's complete adjuvant and some were unilaterally nephrectomized. Anti-GBM antibody glomerulonephritis, characterized by proteinuria (greater than 100 mg/16 h) and a diffuse linear deposition of host immunoglobulin along the glomerular basement membrane, was first detected in Holtzman rats 4 weeks after treatment with GBM had begun, and had developed in 69% of these rats by 15 weeks. In contrast, none of the similarly treated Wistar or HS rats became proteinuric at any time, although a few showed weak glomerular fluorescence at the end of the experiment. Thus Holtzman rats are susceptible, and HS and Wistar rats are resistant to experimental anti-GBM antibody glomerulonephritis. Pretreatment with Freund's complete adjuvant apparently shortened the induction period of the experimental disease in the Holtzman rats whereas unilateral nephrectomy appeared to decrease their susceptibility to it.", "contents": "Experimental autoimmune glomerulonephritis in the rat. I. Factors influencing induction. Albino Holtzman, albino Wistar and hooded HS rats were injected fortnightly for 14 weeks with human glomerular basement membrane (GBM) emulsified in Freund's complete adjuvant. Half of the rats were pretreated with Freund's complete adjuvant and some were unilaterally nephrectomized. Anti-GBM antibody glomerulonephritis, characterized by proteinuria (greater than 100 mg/16 h) and a diffuse linear deposition of host immunoglobulin along the glomerular basement membrane, was first detected in Holtzman rats 4 weeks after treatment with GBM had begun, and had developed in 69% of these rats by 15 weeks. In contrast, none of the similarly treated Wistar or HS rats became proteinuric at any time, although a few showed weak glomerular fluorescence at the end of the experiment. Thus Holtzman rats are susceptible, and HS and Wistar rats are resistant to experimental anti-GBM antibody glomerulonephritis. Pretreatment with Freund's complete adjuvant apparently shortened the induction period of the experimental disease in the Holtzman rats whereas unilateral nephrectomy appeared to decrease their susceptibility to it.", "PMID": 972769}
{"id": "pubmed23n0032_9074", "title": "Experimental autoimmune glomerulonephritis in the rat. II. A histopathological and fine structural study.", "content": "Human glomerular basement membrane (GBM) emulsified in Freund's complete adjuvant was injected into 16 Holtzman rats. Eleven (69%) of them subsequently developed glomerulonephritis. This disease was characterized by glomerular changes which included focal lobular hypercellularity due to localized hyperplasia of intracapillary (mesangial or endothelial) cells which often obstructed glomerular capillaries. Later, affected lobules showed necrosis of intracapillary cells, and capillary lumina were filled with homogeneous eosinophilic material. An immunoperoxidase technique revealed autologous anti-GBM antibody in all 3 layers of the GBM and in some regions it was present in greater amount in the lamina rara interna and externa than in the lamina densa. Experimental auto-immune glomerulonephritis in the rat is thus characterized morphologically by a focal proliferative glomerulonephritis which proceeds to focal glumerulosclerosis.", "contents": "Experimental autoimmune glomerulonephritis in the rat. II. A histopathological and fine structural study. Human glomerular basement membrane (GBM) emulsified in Freund's complete adjuvant was injected into 16 Holtzman rats. Eleven (69%) of them subsequently developed glomerulonephritis. This disease was characterized by glomerular changes which included focal lobular hypercellularity due to localized hyperplasia of intracapillary (mesangial or endothelial) cells which often obstructed glomerular capillaries. Later, affected lobules showed necrosis of intracapillary cells, and capillary lumina were filled with homogeneous eosinophilic material. An immunoperoxidase technique revealed autologous anti-GBM antibody in all 3 layers of the GBM and in some regions it was present in greater amount in the lamina rara interna and externa than in the lamina densa. Experimental auto-immune glomerulonephritis in the rat is thus characterized morphologically by a focal proliferative glomerulonephritis which proceeds to focal glumerulosclerosis.", "PMID": 972770}
{"id": "pubmed23n0032_9075", "title": "Further studies of the acute effects of phenylbutazone, oxyphenbutazone and indomethacin on the rat kidney.", "content": "Experiments were donducted on rats to determine the lowest dose of either phenylbutazone or indomethacin capable of producing papillary necrosis and in each case it was found to be 50 mg/kg body weight. A single dose of oxyphenbutazone (444 mg/kg), a major metabolite of phenylbutazone in man, produced patchy cortical necrosis, which became more extensive during daily administration for 4 days, despite evidence of regeneration. Although papillary necrosis as such was never seen with this substance, there was evidence of damage to the lower nephron in the form of tubular necrosis and calcification.", "contents": "Further studies of the acute effects of phenylbutazone, oxyphenbutazone and indomethacin on the rat kidney. Experiments were donducted on rats to determine the lowest dose of either phenylbutazone or indomethacin capable of producing papillary necrosis and in each case it was found to be 50 mg/kg body weight. A single dose of oxyphenbutazone (444 mg/kg), a major metabolite of phenylbutazone in man, produced patchy cortical necrosis, which became more extensive during daily administration for 4 days, despite evidence of regeneration. Although papillary necrosis as such was never seen with this substance, there was evidence of damage to the lower nephron in the form of tubular necrosis and calcification.", "PMID": 972771}
{"id": "pubmed23n0032_9076", "title": "Oncogenicity tests of p-nitroso-N,N-dimethylaniline and p-nitroso-N,N-diethylaniline in NZR rats and NZO mice.", "content": "Whole-of-life tests of the C-nitroso compounds p-nitrosodimethylaniline (NDMA) and p-nitroso-diethylaniline (NDEA) have been completed in male rats and mice fed maximum tolerated doses continuously over the first halves of their respective natural lifespans. The chemicals were offered at a concentration of 300 mg/litre drinking fluid, but the doses of NDEA consumed were only 75% of the NDMA doses, in both species. Possibly because of this the results with NDEA were statistically not clear-cut, but there was a significant increase in tumour incidence after NDMA treatment in both species. The main sites of tumorigenesis after NDMA were lung, kidney and malignant lymphoma in the rats, and lung, duodenum and malignant lymphoma in the mice. The results confirm our own earlier experiment and provide the first evidence of oncogenic activity in this class of compounds.", "contents": "Oncogenicity tests of p-nitroso-N,N-dimethylaniline and p-nitroso-N,N-diethylaniline in NZR rats and NZO mice. Whole-of-life tests of the C-nitroso compounds p-nitrosodimethylaniline (NDMA) and p-nitroso-diethylaniline (NDEA) have been completed in male rats and mice fed maximum tolerated doses continuously over the first halves of their respective natural lifespans. The chemicals were offered at a concentration of 300 mg/litre drinking fluid, but the doses of NDEA consumed were only 75% of the NDMA doses, in both species. Possibly because of this the results with NDEA were statistically not clear-cut, but there was a significant increase in tumour incidence after NDMA treatment in both species. The main sites of tumorigenesis after NDMA were lung, kidney and malignant lymphoma in the rats, and lung, duodenum and malignant lymphoma in the mice. The results confirm our own earlier experiment and provide the first evidence of oncogenic activity in this class of compounds.", "PMID": 972772}
{"id": "pubmed23n0032_9077", "title": "Serum pyridoxal and folate concentrations in diabetics.", "content": "Serum pyridoxal and folate concentrations were measured in 518 diabetics. There were 185 males and 333 females. The level of pyridoxal was significantly lower in the diabetics when compared with 371 'healthy' controls and 25% had levels below the lower limit of the normal range. No significant difference was observed between diabetics being treated by diet alone, oral hypoglycaemics or insulin. Only 20 patients had a reduced serum folate level and in 6 this was accompanied by a low pyridoxal concentration. The results suggest that diabetics may have an increased demand for pyridoxal.", "contents": "Serum pyridoxal and folate concentrations in diabetics. Serum pyridoxal and folate concentrations were measured in 518 diabetics. There were 185 males and 333 females. The level of pyridoxal was significantly lower in the diabetics when compared with 371 'healthy' controls and 25% had levels below the lower limit of the normal range. No significant difference was observed between diabetics being treated by diet alone, oral hypoglycaemics or insulin. Only 20 patients had a reduced serum folate level and in 6 this was accompanied by a low pyridoxal concentration. The results suggest that diabetics may have an increased demand for pyridoxal.", "PMID": 972773}
{"id": "pubmed23n0032_9078", "title": "An ultrastructural study of the pathogenesis of tissue injury in limited Wegener's granulomatosis.", "content": "Intravascular lysis of leucocytes was an early event in the process of tissue injury in a patient with limited Wegener's granulomatosis. This lysis, with consequent liberation of free organelles into the circulation, was followed by platelet aggregation and fibrin deposition in vessels with intact endothelial cells. Necrosis of endothelial cells also occurred early in the tissue injury. Complete obstruction of vessel lumina, with necrosis of their walls and of alveolar pneumocytes, followed. The alveolar spaces filled with fibrin and cell debris, and macrophages and fibroblasts migrated into these areas. The ultrastructural findings offer a rationale for current therapy. Cyclophosphamide therapy led to improvement in the clinical state, pulmonary function and histological changes.", "contents": "An ultrastructural study of the pathogenesis of tissue injury in limited Wegener's granulomatosis. Intravascular lysis of leucocytes was an early event in the process of tissue injury in a patient with limited Wegener's granulomatosis. This lysis, with consequent liberation of free organelles into the circulation, was followed by platelet aggregation and fibrin deposition in vessels with intact endothelial cells. Necrosis of endothelial cells also occurred early in the tissue injury. Complete obstruction of vessel lumina, with necrosis of their walls and of alveolar pneumocytes, followed. The alveolar spaces filled with fibrin and cell debris, and macrophages and fibroblasts migrated into these areas. The ultrastructural findings offer a rationale for current therapy. Cyclophosphamide therapy led to improvement in the clinical state, pulmonary function and histological changes.", "PMID": 972774}
{"id": "pubmed23n0032_9079", "title": "Haemoglobin C and haemoglobin O Arab-thalassaemia in families of Greek origin.", "content": "A case of Haemoglobin C trait and a family with Haemoglobin O Arab thalassaemia from Greece are described. Both Haemoglobin C and Haemoglobin O Arab were identified by peptide analysis.", "contents": "Haemoglobin C and haemoglobin O Arab-thalassaemia in families of Greek origin. A case of Haemoglobin C trait and a family with Haemoglobin O Arab thalassaemia from Greece are described. Both Haemoglobin C and Haemoglobin O Arab were identified by peptide analysis.", "PMID": 972775}
{"id": "pubmed23n0032_9080", "title": "Haemoglobin Lepore Washington in an Australian family.", "content": "Haemoglobin Lepore Washington is reported apparently for the first time in a white Australian male. Because some of this patient's family have similar blood changes it is assumed that they also have inherited the disorder.", "contents": "Haemoglobin Lepore Washington in an Australian family. Haemoglobin Lepore Washington is reported apparently for the first time in a white Australian male. Because some of this patient's family have similar blood changes it is assumed that they also have inherited the disorder.", "PMID": 972776}
{"id": "pubmed23n0032_9081", "title": "Emergency management of the injured child.", "content": "Now that the crisis situation is past and the child appears to be stable, do not be lulled into a sense of security. Repeated examinations will be necessary to uncover significant internal injuries, such as a subcapsular hematoma of the spleen or liver that gives rise to continuous slow bleeding and may not show signs or symptoms until several hours after the time of injury. the first chest film may be within normal limits. A film several hours later may show the characteristic infiltrates of pulmonary contusion. Arterial blood gases may change before demonstrating other physical signs or symptoms of metabolic or respiratory deficiencies. The primary physician who cares for the multiply injured child must be familiar with the special needs of infants and children. They are not little adults. Dosages of medications differ; these children have special needs to meet for growth and development, and special psychologic needs. In dealing with the very young, do not give up too soon, especially with central nervous system injury. Many of these children have the ability to make a \"\"miraculous'' recovery, with little or no residual damage. I would like to make a special plea for the early use of hyperalimentation solution in the treatment of the multiple injury as a means of promoting optimum growth, development, and healing of the injured child.", "contents": "Emergency management of the injured child. Now that the crisis situation is past and the child appears to be stable, do not be lulled into a sense of security. Repeated examinations will be necessary to uncover significant internal injuries, such as a subcapsular hematoma of the spleen or liver that gives rise to continuous slow bleeding and may not show signs or symptoms until several hours after the time of injury. the first chest film may be within normal limits. A film several hours later may show the characteristic infiltrates of pulmonary contusion. Arterial blood gases may change before demonstrating other physical signs or symptoms of metabolic or respiratory deficiencies. The primary physician who cares for the multiply injured child must be familiar with the special needs of infants and children. They are not little adults. Dosages of medications differ; these children have special needs to meet for growth and development, and special psychologic needs. In dealing with the very young, do not give up too soon, especially with central nervous system injury. Many of these children have the ability to make a \"\"miraculous'' recovery, with little or no residual damage. I would like to make a special plea for the early use of hyperalimentation solution in the treatment of the multiple injury as a means of promoting optimum growth, development, and healing of the injured child.", "PMID": 972779}
{"id": "pubmed23n0032_9082", "title": "The adaptation of the fetal red cells of newborn lambs to extrauterine life: the role of 2,3-diphosphoglycerate and and adult hemoglobin.", "content": "The purpose of this study was to determine the interrelationship of the rise and fall of 2,3-diphosphoglycerate (DPG) with the increase in adult hemoglobin and the decrease in red cell oxygen hemoglobin affinity after birth in normal lambs. It was found that the mean maximum DPG level was 26.71 +/- 4.98 mol/g Hb at 7.5 +/- 1.1 days. At the same time the mean P50 and adult hemoglobin level was 27.0 +/- 1.4 mm Hg and 31.1 +/- 11.i%, respectively. In the individual lambs, the level of their maximum DPG correlated inversely with the amount of adult hemoglobin (r-0.77, P less than 0.05). Once the DPG began to decrease, there was an inverse correlation between the DPG and the adult hemoglobin present in the red cell (r = 0.68, P less than 0.001). It appeared that the rise in DPG postanatally is only a compensatory mechanism until an adequate amount of adult hemoglobin is present. This fact was borne out by the second part of the study in which exchange transfusions with adult red cells were performed on five newborn lambs during the first 24 hr after birth and aborted the rise in DPG.", "contents": "The adaptation of the fetal red cells of newborn lambs to extrauterine life: the role of 2,3-diphosphoglycerate and and adult hemoglobin. The purpose of this study was to determine the interrelationship of the rise and fall of 2,3-diphosphoglycerate (DPG) with the increase in adult hemoglobin and the decrease in red cell oxygen hemoglobin affinity after birth in normal lambs. It was found that the mean maximum DPG level was 26.71 +/- 4.98 mol/g Hb at 7.5 +/- 1.1 days. At the same time the mean P50 and adult hemoglobin level was 27.0 +/- 1.4 mm Hg and 31.1 +/- 11.i%, respectively. In the individual lambs, the level of their maximum DPG correlated inversely with the amount of adult hemoglobin (r-0.77, P less than 0.05). Once the DPG began to decrease, there was an inverse correlation between the DPG and the adult hemoglobin present in the red cell (r = 0.68, P less than 0.001). It appeared that the rise in DPG postanatally is only a compensatory mechanism until an adequate amount of adult hemoglobin is present. This fact was borne out by the second part of the study in which exchange transfusions with adult red cells were performed on five newborn lambs during the first 24 hr after birth and aborted the rise in DPG.", "PMID": 972783}
{"id": "pubmed23n0032_9083", "title": "D-glyceric acidemia: biohcemical studies of a new syndrome.", "content": "Studies of a mentally retarded boy, clinically suffering from nonketotic hyperglycinemia, are reported. Using combined gas chromatography-mass spectrometry, enzyme specificity studies, and spectropolariometry D-glyceric acid in extremely elevated concentrations was demonstrated in both serum and urine (serum: 1.0-1.3 mmol/liter, urine: 33-187 mmol/liter). Hydroxypyruvic acid was not detectable in urine from this boy using a gas chromatographic method with a limit of detection of 0.3 mmol/liter. Enzyme assays of D-glyceric dehydrogenase on blood leukocytes demonstrated significantly lower activity in the patient compared with five normal children.", "contents": "D-glyceric acidemia: biohcemical studies of a new syndrome. Studies of a mentally retarded boy, clinically suffering from nonketotic hyperglycinemia, are reported. Using combined gas chromatography-mass spectrometry, enzyme specificity studies, and spectropolariometry D-glyceric acid in extremely elevated concentrations was demonstrated in both serum and urine (serum: 1.0-1.3 mmol/liter, urine: 33-187 mmol/liter). Hydroxypyruvic acid was not detectable in urine from this boy using a gas chromatographic method with a limit of detection of 0.3 mmol/liter. Enzyme assays of D-glyceric dehydrogenase on blood leukocytes demonstrated significantly lower activity in the patient compared with five normal children.", "PMID": 972784}
{"id": "pubmed23n0032_9084", "title": "A behavioral phenotype in the de Lange syndrome.", "content": "The behavior of nine patients with the de Lange syndrome was studied using videotape, a recording protocol of eight standardized stimulus conditions, and a visual, digital time reference which permitted precise coding and quantitative analysis. These patients avoid or reject social interactions and physical contact, and they do not distinguish in this between a stranger and the mother or her substitute. Social interactions with the adult stranger were scored in patients 1-7 as negative for 28-56 sec/min, whereas they were positive for 1-6 sec/min. Statistical significance was at the level of P less than 0.01. In the case of the mother negative responses ranged from 21-45 sec/min and positive from 3-27 sec/min. The patients exhibit infrequent facial expressions of emotion, and frequently display stereotypic movements. On the other hand, vestibular stimulation or vigorous movement appeared to be an effective means of eliciting pleasurable responses. When held in arms in the vertical position only one of nine children smiled at all and the frequency for that child was 0.8/min. When the child was bounced vigorously in the same position, all smiled but who was too large to be bounced. In the eight the frequency of smiling ranged from 0.8-3.6/min. The data obtained indicate that a specific behavioral phenotype is associated with this syndrome.", "contents": "A behavioral phenotype in the de Lange syndrome. The behavior of nine patients with the de Lange syndrome was studied using videotape, a recording protocol of eight standardized stimulus conditions, and a visual, digital time reference which permitted precise coding and quantitative analysis. These patients avoid or reject social interactions and physical contact, and they do not distinguish in this between a stranger and the mother or her substitute. Social interactions with the adult stranger were scored in patients 1-7 as negative for 28-56 sec/min, whereas they were positive for 1-6 sec/min. Statistical significance was at the level of P less than 0.01. In the case of the mother negative responses ranged from 21-45 sec/min and positive from 3-27 sec/min. The patients exhibit infrequent facial expressions of emotion, and frequently display stereotypic movements. On the other hand, vestibular stimulation or vigorous movement appeared to be an effective means of eliciting pleasurable responses. When held in arms in the vertical position only one of nine children smiled at all and the frequency for that child was 0.8/min. When the child was bounced vigorously in the same position, all smiled but who was too large to be bounced. In the eight the frequency of smiling ranged from 0.8-3.6/min. The data obtained indicate that a specific behavioral phenotype is associated with this syndrome.", "PMID": 972785}
{"id": "pubmed23n0032_9085", "title": "Development of guanylylimidodiphosphate-dependent activation of adenylate cyclase by glucagon in the neonatal rat heart.", "content": "The basal adenylate cyclase activity of the rat heart increases with the age of the animal. By itself, 10(-5) M glucagon activates only adenylate cyclase activity from adult rat hearts. In contrast, 10(-5) M glucagon in the presence of 10(-4)M 5'-guanylylimidodiphosphate (GMP-PNP) clearly activates adenylates cyclase activity in the 14-day-old rat heart, with some activation being evident in hearts of 7-day-old animals. GMP-PNP, 10(-4) M, activates adenylate cyclase activity by itself at ages of 14 days and older, but to a far lesser degree than in combination with 10(-5) M glucagon. Activity elicited by NaF increases throughout the neonatal period. The ratio of NaF-stimulated activity to basal activity increases from 6.3 at 2 days to 10.0 in the adult, a change which is not statistically significant. We conclude that a cardiac receptor for glucagon is present early in neonatal period of the rat, but this receptor cannot effect activation of adenylate cyclase and an increase in heart rate, or depletion of glycogen. Even in the presence of 10(-4) GMP-PNP, the response to glucagon by cardiac adenylate cyclase depends on the age of the rat. In heart cells from a 7-day-old rat, the response is barely measurable but the magnitude of the response increases each week.", "contents": "Development of guanylylimidodiphosphate-dependent activation of adenylate cyclase by glucagon in the neonatal rat heart. The basal adenylate cyclase activity of the rat heart increases with the age of the animal. By itself, 10(-5) M glucagon activates only adenylate cyclase activity from adult rat hearts. In contrast, 10(-5) M glucagon in the presence of 10(-4)M 5'-guanylylimidodiphosphate (GMP-PNP) clearly activates adenylates cyclase activity in the 14-day-old rat heart, with some activation being evident in hearts of 7-day-old animals. GMP-PNP, 10(-4) M, activates adenylate cyclase activity by itself at ages of 14 days and older, but to a far lesser degree than in combination with 10(-5) M glucagon. Activity elicited by NaF increases throughout the neonatal period. The ratio of NaF-stimulated activity to basal activity increases from 6.3 at 2 days to 10.0 in the adult, a change which is not statistically significant. We conclude that a cardiac receptor for glucagon is present early in neonatal period of the rat, but this receptor cannot effect activation of adenylate cyclase and an increase in heart rate, or depletion of glycogen. Even in the presence of 10(-4) GMP-PNP, the response to glucagon by cardiac adenylate cyclase depends on the age of the rat. In heart cells from a 7-day-old rat, the response is barely measurable but the magnitude of the response increases each week.", "PMID": 972786}
{"id": "pubmed23n0032_9086", "title": "Riboflaven and bilirubin response during phototherapy.", "content": "Twenty-four jaundiced neonates were studied, 12 in the treatment group and 12 in the untreated group. Patients were randomly selected to receive oral riboflavin. The mean 24-hr bilirubin decrease was determined during phototherapy. Blue light (420-470 nm) energy ranged from 6-10 muW/cm2. The observed 24-hr bilirubin decreased was compared with the expected decrease based on an energy-dose-response relationship. Riboflavin-treated infants received either 6-7 mu W/cm blue light energy or 810 muW/cm (same as control group). Those infants receiving less energy than the control group (8-10 muW)cm2 had a mean 24-hr bilirubin decrease (3.05 mg/100 ml/24 hr) equal to the control group (3.09 mg/100 ml/24 hr). Those riboflavin-treated infants receiving energy equal to the control group showed a greater decline (5.2 mg/100 ml/24 hr) in their mean 24-hr bilirubin. Although effective, additional in vivo studies are required to clarify the full effects, especially on DNA, of using photosensitizers such as riboflavin in the presence of bilirubin and blue light energy (420-470 nm).", "contents": "Riboflaven and bilirubin response during phototherapy. Twenty-four jaundiced neonates were studied, 12 in the treatment group and 12 in the untreated group. Patients were randomly selected to receive oral riboflavin. The mean 24-hr bilirubin decrease was determined during phototherapy. Blue light (420-470 nm) energy ranged from 6-10 muW/cm2. The observed 24-hr bilirubin decreased was compared with the expected decrease based on an energy-dose-response relationship. Riboflavin-treated infants received either 6-7 mu W/cm blue light energy or 810 muW/cm (same as control group). Those infants receiving less energy than the control group (8-10 muW)cm2 had a mean 24-hr bilirubin decrease (3.05 mg/100 ml/24 hr) equal to the control group (3.09 mg/100 ml/24 hr). Those riboflavin-treated infants receiving energy equal to the control group showed a greater decline (5.2 mg/100 ml/24 hr) in their mean 24-hr bilirubin. Although effective, additional in vivo studies are required to clarify the full effects, especially on DNA, of using photosensitizers such as riboflavin in the presence of bilirubin and blue light energy (420-470 nm).", "PMID": 972787}
{"id": "pubmed23n0032_9087", "title": "Evaluation of the Porta-Warm mattress as a source of heat for neonatal transport.", "content": "One risk of transport of newborns to referral centers is hypothermia. Modern transport incubators have limitations in their ability to keep infants euthermic. As tested in the laboratory and during actual transport the Porta-Warm mattress extends the capability of the transport incubator to keep neonates euthermic by (1) reducing the time required to warm the incubator and (2) warming the incubator in cooler environements. The mattress is an effective adjunct to the transport incubator for keeping newborns warm during transport.", "contents": "Evaluation of the Porta-Warm mattress as a source of heat for neonatal transport. One risk of transport of newborns to referral centers is hypothermia. Modern transport incubators have limitations in their ability to keep infants euthermic. As tested in the laboratory and during actual transport the Porta-Warm mattress extends the capability of the transport incubator to keep neonates euthermic by (1) reducing the time required to warm the incubator and (2) warming the incubator in cooler environements. The mattress is an effective adjunct to the transport incubator for keeping newborns warm during transport.", "PMID": 972791}
{"id": "pubmed23n0032_9088", "title": "Urogenital tract abnormalities in sons of women treated with diethylstilbestrol.", "content": "Since in utero exposure to diethylstilbestrol (DES) is known to cause abnormalities of the female genital tract later in life, exposed male offspring were located, surveyed by mail, and compared with unexposed male offspring from the same period and medical practices. The exposed and unexposed respondents appeared comparable and did not differ in their response to most medical questions. However, a larger proportion of exposed than of unexposed boys had experienced problems in passing urine (12.9% vs. 1.8%, P = .0003) and abnormalities of the penile urethra (4.4% vs. 0%; P = .017).", "contents": "Urogenital tract abnormalities in sons of women treated with diethylstilbestrol. Since in utero exposure to diethylstilbestrol (DES) is known to cause abnormalities of the female genital tract later in life, exposed male offspring were located, surveyed by mail, and compared with unexposed male offspring from the same period and medical practices. The exposed and unexposed respondents appeared comparable and did not differ in their response to most medical questions. However, a larger proportion of exposed than of unexposed boys had experienced problems in passing urine (12.9% vs. 1.8%, P = .0003) and abnormalities of the penile urethra (4.4% vs. 0%; P = .017).", "PMID": 972792}
{"id": "pubmed23n0032_9089", "title": "Solitary thyroid nodules in childhood: is the incidence of thyroid carcinoma declining?", "content": "Solitary thyroid nodules in childhood had a 40% to 70% incidence of malignancy in the era of low-dose therapeutic irradiation. In the last 15 years, 36 children have been evaluated for such nodules, with a final diagnoses of carcinoma in 17%, adenoma in 58%, and miscellaneous diagnoses in 26%. All patients with carcinoma were euthyroid and had no history of irradiation in infancy. Treatment included thyroidectomy and full-replacement thyroid therapy. Benign neoplasms were often \"cold\" by scan; all patients were euthyroid except one adolescent body with T3 toxicosis. A majority proved to be follicular adenomas at surgery. Miscellaneous benign conditions outnumbered carcinoma and included variations in migration or embryologic development of the thyroid anlagen, thyroiditis, and a thyroid abcess. A 99mTc scan proved more valuable in diagnosis than thyroid function tests: a \"hot\" nodule usually suggested a developmental abnormality of the thyroid rather than a tumor. Our series of children, born since the dangers of irradiation have become common knowledge, suggests there may be a changing trend in the pathology encountered in solitary thryoid nodules.", "contents": "Solitary thyroid nodules in childhood: is the incidence of thyroid carcinoma declining? Solitary thyroid nodules in childhood had a 40% to 70% incidence of malignancy in the era of low-dose therapeutic irradiation. In the last 15 years, 36 children have been evaluated for such nodules, with a final diagnoses of carcinoma in 17%, adenoma in 58%, and miscellaneous diagnoses in 26%. All patients with carcinoma were euthyroid and had no history of irradiation in infancy. Treatment included thyroidectomy and full-replacement thyroid therapy. Benign neoplasms were often \"cold\" by scan; all patients were euthyroid except one adolescent body with T3 toxicosis. A majority proved to be follicular adenomas at surgery. Miscellaneous benign conditions outnumbered carcinoma and included variations in migration or embryologic development of the thyroid anlagen, thyroiditis, and a thyroid abcess. A 99mTc scan proved more valuable in diagnosis than thyroid function tests: a \"hot\" nodule usually suggested a developmental abnormality of the thyroid rather than a tumor. Our series of children, born since the dangers of irradiation have become common knowledge, suggests there may be a changing trend in the pathology encountered in solitary thryoid nodules.", "PMID": 972793}
{"id": "pubmed23n0032_9090", "title": "Physical signs in childhood asthma.", "content": "In 62 children with bronchial asthma, the presence of subjective dyspnea and wheeze, and some physical signs commonly associated with chronic obstructive airway disease in older patients, were compared with results of routine pulmonary function tests. Overall, airway resistance and the relationships of residual volume and functiona residual capacity to total lung capacity were increased and other measurements of pulmonary function were moderately decreased. The time-honored subjective dyspnea, wheeze, rhonchi, and prolonged expiration were least useful as indices of severity of disease. Most of the patients, particularly those in whom laboratory testing revealed marked impairment, had notable rhonchi, prolonged expiration, scalene muscle and sternocleidomastoid contraction, and supraclavicular indrawing. Only sternocleidomastoid contraction and supraclavicular indrawing clearly correlated with the severity of airway obstruction. A call is made for a search for these useful signs, whose presence may be the only clue to moderately severe disease; however, their absence does not guarantee absence of severe airway obstruction.", "contents": "Physical signs in childhood asthma. In 62 children with bronchial asthma, the presence of subjective dyspnea and wheeze, and some physical signs commonly associated with chronic obstructive airway disease in older patients, were compared with results of routine pulmonary function tests. Overall, airway resistance and the relationships of residual volume and functiona residual capacity to total lung capacity were increased and other measurements of pulmonary function were moderately decreased. The time-honored subjective dyspnea, wheeze, rhonchi, and prolonged expiration were least useful as indices of severity of disease. Most of the patients, particularly those in whom laboratory testing revealed marked impairment, had notable rhonchi, prolonged expiration, scalene muscle and sternocleidomastoid contraction, and supraclavicular indrawing. Only sternocleidomastoid contraction and supraclavicular indrawing clearly correlated with the severity of airway obstruction. A call is made for a search for these useful signs, whose presence may be the only clue to moderately severe disease; however, their absence does not guarantee absence of severe airway obstruction.", "PMID": 972794}
{"id": "pubmed23n0032_9091", "title": "Pharmacokinetics of theophylline in children with asthma.", "content": "The pharmacokinetics of theophylline following intravenous injection of aminophylline, 4 mg/kg of body weight, were determined in 30 children with asthma and in 6 normal adult volunteers. The average total clearance of theophylline was 87 ml/hr/kg in the children and 57 ml/hr/kg in the adults. The biologic half-life of theophylline in the children ranged from 1.42 to 7.85 hours, reflecting mainly pronounced interindividual differences in the elimination rate constant of the drug. There was no significant difference between the children and adults with respect to the distribution rate constants and apparent volumes of distribution of theophylline, but the elimination rate constant of the drug was considerably higher in children than in adults. Thus, children eliminate theophylline more rapidly on the average than do adults and also show pronounced interindividual differences in the elimination of the drug. Compared to adults, children tend to require relatively larger amounts of theophylline per day and the doses may have to be given at shorter intervals of time.", "contents": "Pharmacokinetics of theophylline in children with asthma. The pharmacokinetics of theophylline following intravenous injection of aminophylline, 4 mg/kg of body weight, were determined in 30 children with asthma and in 6 normal adult volunteers. The average total clearance of theophylline was 87 ml/hr/kg in the children and 57 ml/hr/kg in the adults. The biologic half-life of theophylline in the children ranged from 1.42 to 7.85 hours, reflecting mainly pronounced interindividual differences in the elimination rate constant of the drug. There was no significant difference between the children and adults with respect to the distribution rate constants and apparent volumes of distribution of theophylline, but the elimination rate constant of the drug was considerably higher in children than in adults. Thus, children eliminate theophylline more rapidly on the average than do adults and also show pronounced interindividual differences in the elimination of the drug. Compared to adults, children tend to require relatively larger amounts of theophylline per day and the doses may have to be given at shorter intervals of time.", "PMID": 972795}
{"id": "pubmed23n0032_9092", "title": "Care of the critically ill child: the bleeding neonate.", "content": "Serious bleeding episodes in newborn infants can usually be diagnosed following careful clinical assessment and a few simple laboratory tests. Certain conditions are found almost exclusively in \"sick\" infants, whereas other coagulation abnormalities occur in otherwise \"healthy\" neonates. Successful management of hemorrhage necessitates a correct diagnosis which thereby dictates appropirate therapy. In some cases, such as in DIC, successful outcome ultimately depends on correction of the underlying pathophysiology which triggered the coagulation disturbance.", "contents": "Care of the critically ill child: the bleeding neonate. Serious bleeding episodes in newborn infants can usually be diagnosed following careful clinical assessment and a few simple laboratory tests. Certain conditions are found almost exclusively in \"sick\" infants, whereas other coagulation abnormalities occur in otherwise \"healthy\" neonates. Successful management of hemorrhage necessitates a correct diagnosis which thereby dictates appropirate therapy. In some cases, such as in DIC, successful outcome ultimately depends on correction of the underlying pathophysiology which triggered the coagulation disturbance.", "PMID": 972796}
{"id": "pubmed23n0032_9093", "title": "Jansen's metaphyseal dysostosis.", "content": "A case of Jansen's metaphyseal dysostosis, a rare disorder of endochondral ossification, is described. This is the first case in which the severe radiographic manifestations of this disease were detected at birth. Linear growth is significantly retarded at 2 years of age. Nevertheless, because full ossification with trabeculae is anticipated, early recognition and appropriate management during the phase of active growth should minimize deformities.", "contents": "Jansen's metaphyseal dysostosis. A case of Jansen's metaphyseal dysostosis, a rare disorder of endochondral ossification, is described. This is the first case in which the severe radiographic manifestations of this disease were detected at birth. Linear growth is significantly retarded at 2 years of age. Nevertheless, because full ossification with trabeculae is anticipated, early recognition and appropriate management during the phase of active growth should minimize deformities.", "PMID": 972797}
{"id": "pubmed23n0032_9094", "title": "Hemolytic-uremic syndrome: absence of circulating endotoxin.", "content": "In children with hemolytic-uremic syndrome endotoxin determinations were carried out in the peripheral circulation in order to get evidence for the hypothetical role of endotoxin in the pathogenesis of the disease. For this purpose the Limulus test was used to determine endotoxin activity in 16 patients with hemolytic-uremic syndrome. In the plasma of these patients no endotoxin could be detected above the lower detection limit of 100 pg/ml, although in all patients with septicemia due to gram-negative bacilli the test was positive.", "contents": "Hemolytic-uremic syndrome: absence of circulating endotoxin. In children with hemolytic-uremic syndrome endotoxin determinations were carried out in the peripheral circulation in order to get evidence for the hypothetical role of endotoxin in the pathogenesis of the disease. For this purpose the Limulus test was used to determine endotoxin activity in 16 patients with hemolytic-uremic syndrome. In the plasma of these patients no endotoxin could be detected above the lower detection limit of 100 pg/ml, although in all patients with septicemia due to gram-negative bacilli the test was positive.", "PMID": 972798}
{"id": "pubmed23n0032_9095", "title": "Near-drowning in children: clinical aspects.", "content": "Drowning is the third most common cause of death in the pediatric age group in Florida. The clinical presentation, laboratory data, modes of therapy and outcome of 34 cases of near-drowning in children under the age of 14 years were reviewed. The most common clinical findings on admission were a history of cardiopulmonary resuscitation, tachypnea, pulmonary edema, and acidosis. Twenty-eight (82%) patients survived. Twelve (35%) required mechanical ventilation. Of these only six survived, four of them with severe neurological sequelae. Modern management of respiratory failure is important for the ultimate survival of these patients; however, the final neurological outcome seems to be related to brain damage at the time of the asphyxia rather than to subsequent management.", "contents": "Near-drowning in children: clinical aspects. Drowning is the third most common cause of death in the pediatric age group in Florida. The clinical presentation, laboratory data, modes of therapy and outcome of 34 cases of near-drowning in children under the age of 14 years were reviewed. The most common clinical findings on admission were a history of cardiopulmonary resuscitation, tachypnea, pulmonary edema, and acidosis. Twenty-eight (82%) patients survived. Twelve (35%) required mechanical ventilation. Of these only six survived, four of them with severe neurological sequelae. Modern management of respiratory failure is important for the ultimate survival of these patients; however, the final neurological outcome seems to be related to brain damage at the time of the asphyxia rather than to subsequent management.", "PMID": 972799}
{"id": "pubmed23n0032_9096", "title": "The school nurse practitioner: providing improved health care to children.", "content": "School nurse practitioners have the competence to participate in providing a broad range of direct primary health care and services to the school-age child. Greater utilization of these specially prepared and highly qualified nurse practitioners as providers of health care in the school is a practical and effective method of adding to the quality, availability, and continuity of health care for the school-age child.", "contents": "The school nurse practitioner: providing improved health care to children. School nurse practitioners have the competence to participate in providing a broad range of direct primary health care and services to the school-age child. Greater utilization of these specially prepared and highly qualified nurse practitioners as providers of health care in the school is a practical and effective method of adding to the quality, availability, and continuity of health care for the school-age child.", "PMID": 972800}
{"id": "pubmed23n0032_9097", "title": "Gasoline and flammable and nonflammable clothing studies.", "content": "Studies were conducted on mannequins using gasoline and flammable and nonflammable children's clothing. The results indicate that the flame-resistant standards currently required by law for children's sleepweare lead to much less severe burns.", "contents": "Gasoline and flammable and nonflammable clothing studies. Studies were conducted on mannequins using gasoline and flammable and nonflammable children's clothing. The results indicate that the flame-resistant standards currently required by law for children's sleepweare lead to much less severe burns.", "PMID": 972801}
{"id": "pubmed23n0032_9098", "title": "A pediatric screening examination for psychosocial problems.", "content": "In an effort to avert the cumulative effects of unresolved emotional problems on children's social and school adjustment, a psychosocial phase was added to a pediatric multiphasic examination. Based upon a cumulative stress concept, the screening procedures included child behavior and family stress questionnaires for parents, and abbreviated standard psychological tests for children, administered by specially trained aides. Computerized results were reported to the child's pediatrician. Follow-up by mental health counselors attached to the pediatric clinic was provided for patients identified as being at high risk of serious psychosocial problems. Evidence of validity of the screen, factors affecting the scores, and effectiveness of clinical follow-up of high-risk patients are discussed. Valid semicomputerized screening of school-age children for serious psychosocial problems can be carried out routinely and at relatively low cost by paraprofessional personnel in a pediatric setting. Impediments to effective use of the screening results on the part of both health care provider and patient are discussed.", "contents": "A pediatric screening examination for psychosocial problems. In an effort to avert the cumulative effects of unresolved emotional problems on children's social and school adjustment, a psychosocial phase was added to a pediatric multiphasic examination. Based upon a cumulative stress concept, the screening procedures included child behavior and family stress questionnaires for parents, and abbreviated standard psychological tests for children, administered by specially trained aides. Computerized results were reported to the child's pediatrician. Follow-up by mental health counselors attached to the pediatric clinic was provided for patients identified as being at high risk of serious psychosocial problems. Evidence of validity of the screen, factors affecting the scores, and effectiveness of clinical follow-up of high-risk patients are discussed. Valid semicomputerized screening of school-age children for serious psychosocial problems can be carried out routinely and at relatively low cost by paraprofessional personnel in a pediatric setting. Impediments to effective use of the screening results on the part of both health care provider and patient are discussed.", "PMID": 972802}
{"id": "pubmed23n0032_9099", "title": "Incidence of illness in early group and family day-care.", "content": "The frequency of reported illness in children enrolled in a day-care center was compared to frequency in home-reared children and in children in family day-care homes. Children ranged from 6 to 42 months of age (mean, 21 months). Data on seven categories of illness were collected by bimonthly telephone interviews with parents during two consecutive winters. In the first year of the study gastrointestinal and total illnesses were reported significantly more frequently in children enrolled in the day-care center, though major illnesses were not. In the second year of the study illnesses were significantly more frequently reported in center children though mainly at the younger ages, that is, prior to 2 years of age. The mean numbers of illnesses and symptoms reported per call over the 2 years of the study were 2.56, 1.36, and 1.35 for center, home-reared and family day-care children respectively. Though it has been shown elsewhere that psychological health may be unaffected by early group care, the present findings imply that physical health may be somewhat reduced. It is unknown whether these children will be more resistant to infection at a later age.", "contents": "Incidence of illness in early group and family day-care. The frequency of reported illness in children enrolled in a day-care center was compared to frequency in home-reared children and in children in family day-care homes. Children ranged from 6 to 42 months of age (mean, 21 months). Data on seven categories of illness were collected by bimonthly telephone interviews with parents during two consecutive winters. In the first year of the study gastrointestinal and total illnesses were reported significantly more frequently in children enrolled in the day-care center, though major illnesses were not. In the second year of the study illnesses were significantly more frequently reported in center children though mainly at the younger ages, that is, prior to 2 years of age. The mean numbers of illnesses and symptoms reported per call over the 2 years of the study were 2.56, 1.36, and 1.35 for center, home-reared and family day-care children respectively. Though it has been shown elsewhere that psychological health may be unaffected by early group care, the present findings imply that physical health may be somewhat reduced. It is unknown whether these children will be more resistant to infection at a later age.", "PMID": 972803}
{"id": "pubmed23n0032_9100", "title": "Experience and reason--briefly recorded.", "content": "Solitary pyogenic hepatic abscesses were identified as the cause of fever, abdominal pain, and hepatomegaly in two otherwise normal children who were seen at St. Louis Children's Hospital during the past year. Liver function tests were normal and blood cultures were negative in both patients. These cases illustrate that pyogenic liver abscess may occur in normal children and should be considered whenever fever of unknown origin is associated with abdominal complaints. Only in this way can we hope to improve upon the results cited previously, namely that the majority of liver abscesses remain undiagnosed during life.", "contents": "Experience and reason--briefly recorded. Solitary pyogenic hepatic abscesses were identified as the cause of fever, abdominal pain, and hepatomegaly in two otherwise normal children who were seen at St. Louis Children's Hospital during the past year. Liver function tests were normal and blood cultures were negative in both patients. These cases illustrate that pyogenic liver abscess may occur in normal children and should be considered whenever fever of unknown origin is associated with abdominal complaints. Only in this way can we hope to improve upon the results cited previously, namely that the majority of liver abscesses remain undiagnosed during life.", "PMID": 972804}
{"id": "pubmed23n0032_9101", "title": "Lethal short-limbed chondrodysplasia in early infancy.", "content": "Nineteen cases of chondrodysplastic short-limbed dwarfism in early infancy were studied in the context of current clinical classification based on established radiographic criteria. The histopathologic findings were sufficiently distinctive in most categories to provide additional diagnostic criteria and to contribute to the understanding and delineation of these disorders. 1. Homozygous achondroplasia is distinguished by markedly disturbed endochondral ossification that differs from the pattern seen in typical heterozygous achondroplasia. The physeal chondrocytes contain abundant granules of glycogen. 2. Achondrogenesis, the severest form of chondrodysplasia, exists in 2 types, in which the histopathologic findings appear to be distinctive and diagnostic. Type 1 is characterized by severe disturbance in endochondral ossification. The zone of resting cartilage contains relatively normal matrix, and the chondrocytes contain intracytoplasmic inclusions. The cartilaginous matrix in type 2 is markedly deficient, and the chondrocytes have a large, primitive, mesenchymatous appearance. 3. Thanatophoric dwarfism is associated with disorganized endochondral ossification similar to that in homozygous achondroplasia, but there is no accumulation of glycogen within chondrocytes. Type 2 thanatophoric dwarfism is differentiated from classical type 1 by the presence of cloverleaf skull and histologically by many bone-lined, penetrating vascular canals in the physis and by hyperactive osteoblasts and osteoclasts in the metaphysis. 4. Asphyxiating thoracic dysplasia of Jeune is differentiated histologically into 2 types. Type 1 is characterized by patchy distribution of endochondral ossification in the physis, irregular physeal-metaphyseal junction and large islands of poorly mineralized cartilage in the metaphysis. Type 2 is characterized by uniform distribution of endochondral ossification that is disorganized and is accompanied by advancing cartilage forming latticelike meshwork in the metaphysis. 5. Chondroectodermal dysplasia, which is radiographically similar to asphyxiating thoracic dysplasia, is marked by the presence of large islands of poorly mineralized cartilage in the spongiosa of vertebral bodies. Disorganized endochondral ossification is, however, uniformly distributed, and there is no latticelike advancing cartilage in the metaphysis. 6. Chondrodysplasia punctata is characterized by myxoid and cystic degeneration of physeal and epiphyseal cartilage with focal calcification. Type 2 (Conradi-H\u00fcnermann) has a nearly normal pattern of endochondral ossification, even though there may be severe disturbance and retardation of the process. The columnization of physeal chondrocytes is normal. Type 1 (rhizomelic) differs by having markedly retarded and disorganized endochondral ossification. 7...", "contents": "Lethal short-limbed chondrodysplasia in early infancy. Nineteen cases of chondrodysplastic short-limbed dwarfism in early infancy were studied in the context of current clinical classification based on established radiographic criteria. The histopathologic findings were sufficiently distinctive in most categories to provide additional diagnostic criteria and to contribute to the understanding and delineation of these disorders. 1. Homozygous achondroplasia is distinguished by markedly disturbed endochondral ossification that differs from the pattern seen in typical heterozygous achondroplasia. The physeal chondrocytes contain abundant granules of glycogen. 2. Achondrogenesis, the severest form of chondrodysplasia, exists in 2 types, in which the histopathologic findings appear to be distinctive and diagnostic. Type 1 is characterized by severe disturbance in endochondral ossification. The zone of resting cartilage contains relatively normal matrix, and the chondrocytes contain intracytoplasmic inclusions. The cartilaginous matrix in type 2 is markedly deficient, and the chondrocytes have a large, primitive, mesenchymatous appearance. 3. Thanatophoric dwarfism is associated with disorganized endochondral ossification similar to that in homozygous achondroplasia, but there is no accumulation of glycogen within chondrocytes. Type 2 thanatophoric dwarfism is differentiated from classical type 1 by the presence of cloverleaf skull and histologically by many bone-lined, penetrating vascular canals in the physis and by hyperactive osteoblasts and osteoclasts in the metaphysis. 4. Asphyxiating thoracic dysplasia of Jeune is differentiated histologically into 2 types. Type 1 is characterized by patchy distribution of endochondral ossification in the physis, irregular physeal-metaphyseal junction and large islands of poorly mineralized cartilage in the metaphysis. Type 2 is characterized by uniform distribution of endochondral ossification that is disorganized and is accompanied by advancing cartilage forming latticelike meshwork in the metaphysis. 5. Chondroectodermal dysplasia, which is radiographically similar to asphyxiating thoracic dysplasia, is marked by the presence of large islands of poorly mineralized cartilage in the spongiosa of vertebral bodies. Disorganized endochondral ossification is, however, uniformly distributed, and there is no latticelike advancing cartilage in the metaphysis. 6. Chondrodysplasia punctata is characterized by myxoid and cystic degeneration of physeal and epiphyseal cartilage with focal calcification. Type 2 (Conradi-H\u00fcnermann) has a nearly normal pattern of endochondral ossification, even though there may be severe disturbance and retardation of the process. The columnization of physeal chondrocytes is normal. Type 1 (rhizomelic) differs by having markedly retarded and disorganized endochondral ossification. 7...", "PMID": 972830}
{"id": "pubmed23n0032_9102", "title": "[Anti-HL-A immunization in polytransfused patients according to the antigenic composition of the transfused blood].", "content": "The presence of anti-HL-A antibodies in a polytransfused person very often is a sign of a bad prognosis for survival of renal grafts or for the efficacity of leucocyte transfusion. In this paper, we describe the immunization of 5 patients who have been studied for several years. The number of units transfused as well as antigenic HL-A composition of these units are known for each patient. This enabled us to identify for each antigen, the number of blood units transfused (results given in %). Our observations confirm facts already described by several authors, study of voluntary programmed immunization, with the cells from the same donor. Furthermore, we are aware that there does not appear to be any direct relationship between proportion (absolute value) of the antigens received and specificity of antibodies. The time needed for an individual to become immunized does not appear to be related to the number of transfusions. This could lead to the modification of the criteria allowing to classify individuals as \"responders\" or \"non-responders\".", "contents": "[Anti-HL-A immunization in polytransfused patients according to the antigenic composition of the transfused blood]. The presence of anti-HL-A antibodies in a polytransfused person very often is a sign of a bad prognosis for survival of renal grafts or for the efficacity of leucocyte transfusion. In this paper, we describe the immunization of 5 patients who have been studied for several years. The number of units transfused as well as antigenic HL-A composition of these units are known for each patient. This enabled us to identify for each antigen, the number of blood units transfused (results given in %). Our observations confirm facts already described by several authors, study of voluntary programmed immunization, with the cells from the same donor. Furthermore, we are aware that there does not appear to be any direct relationship between proportion (absolute value) of the antigens received and specificity of antibodies. The time needed for an individual to become immunized does not appear to be related to the number of transfusions. This could lead to the modification of the criteria allowing to classify individuals as \"responders\" or \"non-responders\".", "PMID": 972831}
{"id": "pubmed23n0032_9103", "title": "The erythrocyte-granulocyte rosette. An immune cellular interaction found in some cases of autoimmune hemolytic anemias.", "content": "Erythrocyte-granulocyte rosettes (EGR) were found in the capillary blood of two cases of autoimmune haemolytic anemia, the first caused by an IgM non-I antibody and the second by an IgG. Both antibodies activated complement, and C3b was demonstrated on the erythrocytes. The rosettes appeared as arrangements of 6-10 red cells adhering to a central granulocyte; phagocytosis was most generally absent. The finding of EGR in the capillary blood may be useful for the recognition of complement activation up to the C3b stage in some cases of autoimmune haemolytic anemia.", "contents": "The erythrocyte-granulocyte rosette. An immune cellular interaction found in some cases of autoimmune hemolytic anemias. Erythrocyte-granulocyte rosettes (EGR) were found in the capillary blood of two cases of autoimmune haemolytic anemia, the first caused by an IgM non-I antibody and the second by an IgG. Both antibodies activated complement, and C3b was demonstrated on the erythrocytes. The rosettes appeared as arrangements of 6-10 red cells adhering to a central granulocyte; phagocytosis was most generally absent. The finding of EGR in the capillary blood may be useful for the recognition of complement activation up to the C3b stage in some cases of autoimmune haemolytic anemia.", "PMID": 972832}
{"id": "pubmed23n0032_9104", "title": "Volumes and cellularity in populations of hematopoietic clones.", "content": "Volumes of hematopoietic spleen colonies were estimated by measuring parameters of superimposed triaxial ellipsoids. Observations were made on groups of colonies that contained only undifferentiated cells or had differentiated to varying degrees into purely erythropoietic, purely granulocytic or purely megakaryocytic colonies. The average volume occupied by one cell and surrounding matrix, the so-called single cell space, was determined for each type of colony. The estimated colony cellularity was then computed from the measured colony volume and the tabulated single cell space. The techniques of measurement are described. Formulas are provided to estimate mean and variance of volumes and their cell contents. Data on the distribution of spleen colony size and cellularity after seven days of growth are given.", "contents": "Volumes and cellularity in populations of hematopoietic clones. Volumes of hematopoietic spleen colonies were estimated by measuring parameters of superimposed triaxial ellipsoids. Observations were made on groups of colonies that contained only undifferentiated cells or had differentiated to varying degrees into purely erythropoietic, purely granulocytic or purely megakaryocytic colonies. The average volume occupied by one cell and surrounding matrix, the so-called single cell space, was determined for each type of colony. The estimated colony cellularity was then computed from the measured colony volume and the tabulated single cell space. The techniques of measurement are described. Formulas are provided to estimate mean and variance of volumes and their cell contents. Data on the distribution of spleen colony size and cellularity after seven days of growth are given.", "PMID": 972833}
{"id": "pubmed23n0032_9105", "title": "Separation of Met-tRNAf and Met-tRNAm by chromatography on Sepharose 4B columns.", "content": "Unfractionated rabbit liver tRNA, charged with [3H]methionine by use of rat liver enzymes, was separated into two [3H5methionine-containing fractions by column chromatography on Sepharose 4B. The two fractions were identified as Met-tRNAMetm and Met-tRNAMetf by (a) their different ability for form a GTP-dependent ternary complex with IF-MP, and (b) the absence of the first fraction after selective charging of the tRNA with E. coli amino acyl tRNA synthetase. The methionine residue was without noticeable influence on the separation.", "contents": "Separation of Met-tRNAf and Met-tRNAm by chromatography on Sepharose 4B columns. Unfractionated rabbit liver tRNA, charged with [3H]methionine by use of rat liver enzymes, was separated into two [3H5methionine-containing fractions by column chromatography on Sepharose 4B. The two fractions were identified as Met-tRNAMetm and Met-tRNAMetf by (a) their different ability for form a GTP-dependent ternary complex with IF-MP, and (b) the absence of the first fraction after selective charging of the tRNA with E. coli amino acyl tRNA synthetase. The methionine residue was without noticeable influence on the separation.", "PMID": 972853}
{"id": "pubmed23n0032_9106", "title": "Affinity chromatography of thymidine kinase from a rat colon adenocarcinoma.", "content": "Thymidine kinase from a transplantable colon adenocarcinoma, induced by 1,2-dimethylhydrazine and maintained in CDF rats, was purified by affinity chromatography using thymidine-3'(4-aminophenylphosphate) coupled to carboxyhexyl-Sepharose. Most of the contaminating protein passed through the column; non-specifically adsorbed protein was washed from the column by 0.1 M KC1 in 0.01 M Tris-HC1, 7.5. Thymidine kinase was eluted with 0.1 mM thymidine, 0.1 M KC1 in 0.01 M Tris-HC1, pH 7.5. The purified enzyme accounted for about 26% of the applied activity; the specific activity of the purified material (peak fraction) was 3,500 moles TMP formed per mg protein per 10 min., a 1,800-fold purification of the applied extract. The preparation is free of nucleoside phosphotransferase, but contains other protein impurities. Purification was completed in less than 1 hour, making this a useful procedure for isolation of this unstable enzyme.", "contents": "Affinity chromatography of thymidine kinase from a rat colon adenocarcinoma. Thymidine kinase from a transplantable colon adenocarcinoma, induced by 1,2-dimethylhydrazine and maintained in CDF rats, was purified by affinity chromatography using thymidine-3'(4-aminophenylphosphate) coupled to carboxyhexyl-Sepharose. Most of the contaminating protein passed through the column; non-specifically adsorbed protein was washed from the column by 0.1 M KC1 in 0.01 M Tris-HC1, 7.5. Thymidine kinase was eluted with 0.1 mM thymidine, 0.1 M KC1 in 0.01 M Tris-HC1, pH 7.5. The purified enzyme accounted for about 26% of the applied activity; the specific activity of the purified material (peak fraction) was 3,500 moles TMP formed per mg protein per 10 min., a 1,800-fold purification of the applied extract. The preparation is free of nucleoside phosphotransferase, but contains other protein impurities. Purification was completed in less than 1 hour, making this a useful procedure for isolation of this unstable enzyme.", "PMID": 972854}
{"id": "pubmed23n0032_9107", "title": "Ion-affinity electrophoresis. Preparation and properties of phosphorylated polyacrylamide gels.", "content": "Strong, pliable polyacrylamide gels containing covalently bound phosphate groups have been formed by radical-initiated copolymerization of acrylamide and a phosphorylated, N-substituted derivative of acrylamide. Under conditions of disc electrophoresis, the gels provide enhanced resolution in the separation of hemoglobin A from S, and separate two forms of yeast glucose 6-phosphate dehydrogenase and rabbit muscle glyceraldehyde 3-phosphate dehydrogenase. Preliminary evidence suggests that the improved resolution is due to ionic adsorption of the proteins to immobilized phosphate groups, implying that the gels may function as supports for electrophoretically powered separations involving ion-exchange or affinity chromatography.", "contents": "Ion-affinity electrophoresis. Preparation and properties of phosphorylated polyacrylamide gels. Strong, pliable polyacrylamide gels containing covalently bound phosphate groups have been formed by radical-initiated copolymerization of acrylamide and a phosphorylated, N-substituted derivative of acrylamide. Under conditions of disc electrophoresis, the gels provide enhanced resolution in the separation of hemoglobin A from S, and separate two forms of yeast glucose 6-phosphate dehydrogenase and rabbit muscle glyceraldehyde 3-phosphate dehydrogenase. Preliminary evidence suggests that the improved resolution is due to ionic adsorption of the proteins to immobilized phosphate groups, implying that the gels may function as supports for electrophoretically powered separations involving ion-exchange or affinity chromatography.", "PMID": 972855}
{"id": "pubmed23n0032_9108", "title": "Systematic evaluation of cellular radiosensitivity parameters.", "content": "Cellular radiosensitivity parameters of the track structure theory of Katz and co-workers are evaluated from a sum of squares minimizing computer program for nonlinear models. Based on these observations, suggestions are presented for efficient experiment design for the determination of these parameters from track-segment bombardments of high LET radiations.", "contents": "Systematic evaluation of cellular radiosensitivity parameters. Cellular radiosensitivity parameters of the track structure theory of Katz and co-workers are evaluated from a sum of squares minimizing computer program for nonlinear models. Based on these observations, suggestions are presented for efficient experiment design for the determination of these parameters from track-segment bombardments of high LET radiations.", "PMID": 972915}
{"id": "pubmed23n0032_9109", "title": "Dosimetry for irregular shaped fields of beta rays.", "content": "The feasibility of using various shapes and sizes of field limiting devices and collimators with beta-ray eye applicators has necessitated the study of dosimetry for these fields. A method of calculating surface and depth doses for any shaped field from the data for circular fields is presented. The depth dose evaluation is based on a measured dose function which is defined as the dose rate at a particular depth for a particular circular field. The evaluated values for the surface and depth dose were compared with experimentally obtained values for three non-circular fields. The good agreement in these data indicates the practicability of the method suggested.", "contents": "Dosimetry for irregular shaped fields of beta rays. The feasibility of using various shapes and sizes of field limiting devices and collimators with beta-ray eye applicators has necessitated the study of dosimetry for these fields. A method of calculating surface and depth doses for any shaped field from the data for circular fields is presented. The depth dose evaluation is based on a measured dose function which is defined as the dose rate at a particular depth for a particular circular field. The evaluated values for the surface and depth dose were compared with experimentally obtained values for three non-circular fields. The good agreement in these data indicates the practicability of the method suggested.", "PMID": 972916}
{"id": "pubmed23n0032_9110", "title": "Transport calculations of gamma ray flux density and dose rate about implantable californium-252 sources.", "content": "Gamma flux density and dose rate distributions have been calculated about implantable californium-252 sources for an infinite tissue medium. Point source flux densities as a function of energy and position were obtained from a discrete-ordinates calculation, and the flux densities were multiplied by their corresponding kerma factors and added to obtain point source dose rates. The point dose rates were integrated over the line source to obtain line source dose rates. Container attenuation was accounted for by evaluating the point dose rate as a function of platinum thickness. Both primary and secondary flux densities and dose rates are presented. The agreement with an independent Monte Carlo calculation was excellent. The data presented should be useful for the design of new source configurations.", "contents": "Transport calculations of gamma ray flux density and dose rate about implantable californium-252 sources. Gamma flux density and dose rate distributions have been calculated about implantable californium-252 sources for an infinite tissue medium. Point source flux densities as a function of energy and position were obtained from a discrete-ordinates calculation, and the flux densities were multiplied by their corresponding kerma factors and added to obtain point source dose rates. The point dose rates were integrated over the line source to obtain line source dose rates. Container attenuation was accounted for by evaluating the point dose rate as a function of platinum thickness. Both primary and secondary flux densities and dose rates are presented. The agreement with an independent Monte Carlo calculation was excellent. The data presented should be useful for the design of new source configurations.", "PMID": 972917}
{"id": "pubmed23n0032_9111", "title": "Conversion from exposure to dose for megavoltage beams under 3 MeV.", "content": "An exact expression was developed for conversion from exposure in air to dose in some medium at the depth where the kerma equals the dose. This depth was estimated for 60Co beams in water using published data, and found to be 0-4 +/- 0-05 cm. An example of the use of the conversion relationship for a 60Co beam is given.", "contents": "Conversion from exposure to dose for megavoltage beams under 3 MeV. An exact expression was developed for conversion from exposure in air to dose in some medium at the depth where the kerma equals the dose. This depth was estimated for 60Co beams in water using published data, and found to be 0-4 +/- 0-05 cm. An example of the use of the conversion relationship for a 60Co beam is given.", "PMID": 972918}
{"id": "pubmed23n0032_9112", "title": "Attenuation measurements of a fast neutron radiotherapy beam.", "content": "Attenuation measurements have been made for pressed wood, steel and lead in the neutron beam generated by 35 MeV deuterons on a thick Be target, using narrow-beam, semibroad-beam and broad-beam geometries. In addition, the measurements were extended to the neutron beams from 25 and 15 MeV D+ on Be, for semibroad-beam geometry only. A tissue-equivalent plastic ionization chamber was used as the detector; hence the measured parameter approximated the total (n + gamma) dose in tissue. After correcting for radiation scattering around the absorbers, the remaining curves were nearly exponential in most cases, and a build-up factor could be employed to describe the varying degree of scattered radiation dose as a function of absorber thickness and geometry. The results given should be applicable in designing primary-beam shields and collimators, as well as providing a useful basis for extending calculations to other geometries through Monte Carlo techniques.", "contents": "Attenuation measurements of a fast neutron radiotherapy beam. Attenuation measurements have been made for pressed wood, steel and lead in the neutron beam generated by 35 MeV deuterons on a thick Be target, using narrow-beam, semibroad-beam and broad-beam geometries. In addition, the measurements were extended to the neutron beams from 25 and 15 MeV D+ on Be, for semibroad-beam geometry only. A tissue-equivalent plastic ionization chamber was used as the detector; hence the measured parameter approximated the total (n + gamma) dose in tissue. After correcting for radiation scattering around the absorbers, the remaining curves were nearly exponential in most cases, and a build-up factor could be employed to describe the varying degree of scattered radiation dose as a function of absorber thickness and geometry. The results given should be applicable in designing primary-beam shields and collimators, as well as providing a useful basis for extending calculations to other geometries through Monte Carlo techniques.", "PMID": 972919}
{"id": "pubmed23n0032_9113", "title": "Physics of contrast mechanism and averaging effect of linear attenuation coefficients in a computerized transverse axial tomography CTAT) transmission scanner.", "content": "Detailed studies of the basic contrast mechanisms in computerized transverse axial tomography scanners have been carried out. Contrast is related to the effective atomic numbers and electron densities of materials and the resultant linear attenuation coefficients. We have therefore quantitatively evaluated various samples defined by these parameters. A multienergetic X-ray source causes resolution degrading problems arising from the averaging effect of the linear attenuation coefficients. The controversy regarding the use of fixed length water bath as a reference to compensate the spectral shift (hardening) effect of the multienergetic X-ray source is also analysed and reported. Computer simulations demonstrating the sensitivities of the linear attenuation coefficient measurements and errors, as functions of the energy spectrum, were made for representative cases. Simulation results indicate that by using a full water bath, artifacts stemming from the multienergetic X-ray souce can be significantly reduced. An alternative approach using a count rate equalizer, considered to be another way of reducing the wide dynamic range in count rate when a water bath is not used, is also studied and the results reported.", "contents": "Physics of contrast mechanism and averaging effect of linear attenuation coefficients in a computerized transverse axial tomography CTAT) transmission scanner. Detailed studies of the basic contrast mechanisms in computerized transverse axial tomography scanners have been carried out. Contrast is related to the effective atomic numbers and electron densities of materials and the resultant linear attenuation coefficients. We have therefore quantitatively evaluated various samples defined by these parameters. A multienergetic X-ray source causes resolution degrading problems arising from the averaging effect of the linear attenuation coefficients. The controversy regarding the use of fixed length water bath as a reference to compensate the spectral shift (hardening) effect of the multienergetic X-ray source is also analysed and reported. Computer simulations demonstrating the sensitivities of the linear attenuation coefficient measurements and errors, as functions of the energy spectrum, were made for representative cases. Simulation results indicate that by using a full water bath, artifacts stemming from the multienergetic X-ray souce can be significantly reduced. An alternative approach using a count rate equalizer, considered to be another way of reducing the wide dynamic range in count rate when a water bath is not used, is also studied and the results reported.", "PMID": 972920}
{"id": "pubmed23n0032_9114", "title": "Quantitative proton tomography: preliminary experiments.", "content": "An attempt has been made to see whether energetic protons (158 MeV) could be used instead of X-rays in computerized axial tomography to detect density differences of the order of those at which commercial X-ray tomographs cease to be useful. A circularly symmetrical phantom consisting of Lucite and sugar solutions was used, and density differences of 0-5% were reconstructed with reasonable accuracy from data obtained with very simple equipment. Discontinuities in either density or chemical composition, or both, seem to cause artifacts in the reconstruction. These may be related to the West-Sherwood effect.", "contents": "Quantitative proton tomography: preliminary experiments. An attempt has been made to see whether energetic protons (158 MeV) could be used instead of X-rays in computerized axial tomography to detect density differences of the order of those at which commercial X-ray tomographs cease to be useful. A circularly symmetrical phantom consisting of Lucite and sugar solutions was used, and density differences of 0-5% were reconstructed with reasonable accuracy from data obtained with very simple equipment. Discontinuities in either density or chemical composition, or both, seem to cause artifacts in the reconstruction. These may be related to the West-Sherwood effect.", "PMID": 972921}
{"id": "pubmed23n0032_9115", "title": "An improved method for detecting passive pills.", "content": "One of the principal disadvantages of the passive pill as a telemetric method for measuring various physiological parameters has been its resticted range. The reasons for the restricted range with existing detection methods are discussed. An improved method using a locking spectrometer based on third-order phase-sensitive detection is described and its performance is assessed. A significant increase in the usable range of a high sensitivity passive pill is obtained.", "contents": "An improved method for detecting passive pills. One of the principal disadvantages of the passive pill as a telemetric method for measuring various physiological parameters has been its resticted range. The reasons for the restricted range with existing detection methods are discussed. An improved method using a locking spectrometer based on third-order phase-sensitive detection is described and its performance is assessed. A significant increase in the usable range of a high sensitivity passive pill is obtained.", "PMID": 972923}
{"id": "pubmed23n0032_9116", "title": "Measurements of trabecular bone structure in man.", "content": "A quantitative analysis of trabecular bone structure is presented, based on omnidirectional distributions of paths across (a) trabeculation and (b) marrow cavities. The omnidirectional distributions, which take into account structural anisotropy, are generated from measured distributions of paths. Representative examples are given, together with values of two commonly quoted structural parameters, the ratio of endosteal surface to bone volume and percentage bone volume. Data on the biological and age variations in the third lumbar vertebra are also presented and an index of trabecular anisotropy suggested. Finally, the results are compared with those of other workers and estimates of useful skeletal parameters given.", "contents": "Measurements of trabecular bone structure in man. A quantitative analysis of trabecular bone structure is presented, based on omnidirectional distributions of paths across (a) trabeculation and (b) marrow cavities. The omnidirectional distributions, which take into account structural anisotropy, are generated from measured distributions of paths. Representative examples are given, together with values of two commonly quoted structural parameters, the ratio of endosteal surface to bone volume and percentage bone volume. Data on the biological and age variations in the third lumbar vertebra are also presented and an index of trabecular anisotropy suggested. Finally, the results are compared with those of other workers and estimates of useful skeletal parameters given.", "PMID": 972924}
{"id": "pubmed23n0032_9117", "title": "Determination of the efficacy of the mechanism changing the relative rate of blood flow through cutaneous and deep lying veins with temperature.", "content": "An analytical model of the circulation in the upper extremities of man is used; it consists of one artery, a deep vein lying close to the artery and returning a fraction p of the arterial blood flow, and a cutaneous vein carrying the fraction (1 - p). Using the model, a method is given for finding, for any subject, the efficiency of the mechanism which changes the circulation pattern with temperature. A patient's extremity is immersed in water at 25 and 10 degrees c and the rate of blood flow and rate of heat loss from that flow are measured. Computation of p for these two temperatures is reduced to the solution of two linear equations. The method is applied to 11 subjects and gives good agreement with results obtained by a more elaborate method.", "contents": "Determination of the efficacy of the mechanism changing the relative rate of blood flow through cutaneous and deep lying veins with temperature. An analytical model of the circulation in the upper extremities of man is used; it consists of one artery, a deep vein lying close to the artery and returning a fraction p of the arterial blood flow, and a cutaneous vein carrying the fraction (1 - p). Using the model, a method is given for finding, for any subject, the efficiency of the mechanism which changes the circulation pattern with temperature. A patient's extremity is immersed in water at 25 and 10 degrees c and the rate of blood flow and rate of heat loss from that flow are measured. Computation of p for these two temperatures is reduced to the solution of two linear equations. The method is applied to 11 subjects and gives good agreement with results obtained by a more elaborate method.", "PMID": 972925}
{"id": "pubmed23n0032_9118", "title": "Calculation of retinal temperature distributions resulting from laser irradiation of the eye: I. Continuous lasers.", "content": "The paper develops a simple analytic expression for the maximum temperature rise attained in the retina as a function of laser power, exposure duration, retinal absorption parameters and image radius. It is assumed that the laser beam has a Gaussian intensity distribution and is absorbed, following Beer's law, in both the pigment epithelium and the choroid layers of the eye. The final expression is mathematically accurate to 1% and is valid for image radii ranging from 20 to 500 mum and for exposure times from 30 ms to infinity. Consequently, this analysis is ideally suited to the interpretation of laser threshold damage experiments using shuttered gas lasers operated in their uniphase mode. Finally, the problem of defining a safe exposure level to laser radiation is considered. However, the lack of available information on minimum retinal image diameter and maximum absorption coefficient permits only an estimate of this parameter to be made.", "contents": "Calculation of retinal temperature distributions resulting from laser irradiation of the eye: I. Continuous lasers. The paper develops a simple analytic expression for the maximum temperature rise attained in the retina as a function of laser power, exposure duration, retinal absorption parameters and image radius. It is assumed that the laser beam has a Gaussian intensity distribution and is absorbed, following Beer's law, in both the pigment epithelium and the choroid layers of the eye. The final expression is mathematically accurate to 1% and is valid for image radii ranging from 20 to 500 mum and for exposure times from 30 ms to infinity. Consequently, this analysis is ideally suited to the interpretation of laser threshold damage experiments using shuttered gas lasers operated in their uniphase mode. Finally, the problem of defining a safe exposure level to laser radiation is considered. However, the lack of available information on minimum retinal image diameter and maximum absorption coefficient permits only an estimate of this parameter to be made.", "PMID": 972926}
{"id": "pubmed23n0032_9119", "title": "Follow-up study of patients treated by x-ray epilation for tinea capitis. Estimation of the dose to the thyroid and pituitary glands and other structures of the head and neck.", "content": "This study is a further investigation of radiation dose to various head structures in the children given X-ray therapy for tinea capitis (ringworm of the scalp). In this work, estimates of the dose to the thyroid and pituitary gland were obtained with lithium fluoride thermoluminescent dosemeters using a child's head phantom. Doses were also measured for the parotid gland and several skin sites where skin tumours developed in the irradiated cases. In a previous study, brain and scalp doses of 140 and 500-800 rad had been estimated for the treated group using this same head phantom. In this work dosemeters were also placed in the same brain locations so that comparisons could be obtained between the two studies. The thyroid dose was estimated to be 6 +/- 2 rad and the pituitary dose was 49 +/- 6 rad for the conventional tinea capitis treatment. The dose to the parotid gland was 39 rad and the dose to skin sites on the face and neck where tumours occurred ranged from 20 to 40 rad. The data for the thyroid adenoma response from this and other studies involving irradiation of children suggests a linear dose-response relationship within the first 30-40 years after exposure with a risk of about 0-04% per rad.", "contents": "Follow-up study of patients treated by x-ray epilation for tinea capitis. Estimation of the dose to the thyroid and pituitary glands and other structures of the head and neck. This study is a further investigation of radiation dose to various head structures in the children given X-ray therapy for tinea capitis (ringworm of the scalp). In this work, estimates of the dose to the thyroid and pituitary gland were obtained with lithium fluoride thermoluminescent dosemeters using a child's head phantom. Doses were also measured for the parotid gland and several skin sites where skin tumours developed in the irradiated cases. In a previous study, brain and scalp doses of 140 and 500-800 rad had been estimated for the treated group using this same head phantom. In this work dosemeters were also placed in the same brain locations so that comparisons could be obtained between the two studies. The thyroid dose was estimated to be 6 +/- 2 rad and the pituitary dose was 49 +/- 6 rad for the conventional tinea capitis treatment. The dose to the parotid gland was 39 rad and the dose to skin sites on the face and neck where tumours occurred ranged from 20 to 40 rad. The data for the thyroid adenoma response from this and other studies involving irradiation of children suggests a linear dose-response relationship within the first 30-40 years after exposure with a risk of about 0-04% per rad.", "PMID": 972927}
{"id": "pubmed23n0032_9120", "title": "Radioimmunoassay of prostaglandins E1, E2, and F2alpha in unextracted plasma, serum and myocardium.", "content": "A radioimmunoassay procedure for the determination of PGE1, PGE2, and PGF2alpha is presented. The procedure involves the pre-precipitation of each prostaglandin specific antiserum with the precipitating antisera (ARGG), and the use of these antisera mixtures in assaying for PGE1, PGE2, and PGF2alpha. Applicability of the methods to unextracted plasma, serum and myocardial homogenate has been demonstrated through tests of specificity, recovery, reproducability and parallelism. A mathematical correction for cross-reactivity between PGE1 and PGE2, and their opposing antisera is given. To demonstrate the utility of the methodology in differentiation of experimental variables, prostaglandin concentrations in unincubated serum, incubated serum, and the rate of prostaglandin production in serum of dogs are given.", "contents": "Radioimmunoassay of prostaglandins E1, E2, and F2alpha in unextracted plasma, serum and myocardium. A radioimmunoassay procedure for the determination of PGE1, PGE2, and PGF2alpha is presented. The procedure involves the pre-precipitation of each prostaglandin specific antiserum with the precipitating antisera (ARGG), and the use of these antisera mixtures in assaying for PGE1, PGE2, and PGF2alpha. Applicability of the methods to unextracted plasma, serum and myocardial homogenate has been demonstrated through tests of specificity, recovery, reproducability and parallelism. A mathematical correction for cross-reactivity between PGE1 and PGE2, and their opposing antisera is given. To demonstrate the utility of the methodology in differentiation of experimental variables, prostaglandin concentrations in unincubated serum, incubated serum, and the rate of prostaglandin production in serum of dogs are given.", "PMID": 972998}
{"id": "pubmed23n0032_9121", "title": "PGE2: opposite effect on catecholamine release from phaeochromocytoma and adrenal medulla.", "content": "The effect of PGE2 on catecholamine release from human adrenal medulla and phaechromocytoma was studied in slices incubated in vitro. In each of 3 normal human adrenal medullae PGE2 (10(-7) M) caused a significant inhibition of the release of catecholamines in in vitro incubation. In each of 3 phaechromocytomas studied PGE2 (10(-7) M) caused a significant increase of catecholamine release in in-vitro incubation. The possible relevance of this mechanism to the regulation of catecholamine release in phaeochromocytoma is discussed.", "contents": "PGE2: opposite effect on catecholamine release from phaeochromocytoma and adrenal medulla. The effect of PGE2 on catecholamine release from human adrenal medulla and phaechromocytoma was studied in slices incubated in vitro. In each of 3 normal human adrenal medullae PGE2 (10(-7) M) caused a significant inhibition of the release of catecholamines in in vitro incubation. In each of 3 phaechromocytomas studied PGE2 (10(-7) M) caused a significant increase of catecholamine release in in-vitro incubation. The possible relevance of this mechanism to the regulation of catecholamine release in phaeochromocytoma is discussed.", "PMID": 972999}
{"id": "pubmed23n0032_9122", "title": "The effect of PGE2 and PGF2alpha on the response of guinea pig myometrium to adenine nucleotides in vitro.", "content": "Prostaglandins E2 and F2alpha potentiate contractile effect induced by adenine nucleotides ATP, ADP and AMP in guinea pig myometrium in vitro. Prostaglandins and nucleotides were added to the organ bath in minute concentrations which have been proved ineffective or slightly contractile when both groups of substances were administered separately. The data of the present work, together with our previously published studies (9, 10, 13), where the action of exogenous adenine nucleotides, NAD and adenosine on rabbit's jejunum in vitro has been proved antagonistic to the contractile effect of various prostaglandins, suggest that prostaglandins and adenine nucleotides appear to block selectively or augment each other's action on various organs. The initial hypothesis that there is a regulatory correlation between endogenous prostaglandins and the function of purinergic nerves also is reinforced.", "contents": "The effect of PGE2 and PGF2alpha on the response of guinea pig myometrium to adenine nucleotides in vitro. Prostaglandins E2 and F2alpha potentiate contractile effect induced by adenine nucleotides ATP, ADP and AMP in guinea pig myometrium in vitro. Prostaglandins and nucleotides were added to the organ bath in minute concentrations which have been proved ineffective or slightly contractile when both groups of substances were administered separately. The data of the present work, together with our previously published studies (9, 10, 13), where the action of exogenous adenine nucleotides, NAD and adenosine on rabbit's jejunum in vitro has been proved antagonistic to the contractile effect of various prostaglandins, suggest that prostaglandins and adenine nucleotides appear to block selectively or augment each other's action on various organs. The initial hypothesis that there is a regulatory correlation between endogenous prostaglandins and the function of purinergic nerves also is reinforced.", "PMID": 973001}
{"id": "pubmed23n0032_9123", "title": "The possible mode of action of prostaglandins: X. Antagonism between prostaglandin F2a and prolactin or human chorionic gonadotropin: a comparative study.", "content": "A comparative assessment of the efficacy of prolactin or HCG in reversing the luteolytic property of a single dose (2.0 mg/kg) of PGF2a was conducted in pregnant (day 10) rats. It was found that prolactin maintained pregnancy in 90% of the test animals. HCG though found to maintain pregnancy in 70% of the PGF2a-treated pregnants, the fetal survival rate was, however, recorded to be 62% against the control value of 98%. Conversely, prolactin replacement maintained the fetal survival rate as high as 93.4%. Moreover, the growth of the fetuses, placentae and corpus luteum in the pregnants having prolactin in conjunction with PGF2a was also found to be greater compared to the animals which had a combined regimen of PGF2a and HCG, but identical to controls. On the other hand, similar combined regimen when applied to hysterectomized pregnant rats, it was observed that though the vaginal diestrus was maintained by the prolactin or HCG in the presence of PGF2a, the prolactin regimen was found to be superb compared to HCG in the maintenance of luteal weight and functional activity. It was concluded that the antifertility effect of PGF2a in the rat is primarily the consequence of luteolysis and prolactin seems to be a much more appropriate hormonal replacement compared to HCG, a long-acting LH, in antagonizing the luteolytic property of PGF2a.", "contents": "The possible mode of action of prostaglandins: X. Antagonism between prostaglandin F2a and prolactin or human chorionic gonadotropin: a comparative study. A comparative assessment of the efficacy of prolactin or HCG in reversing the luteolytic property of a single dose (2.0 mg/kg) of PGF2a was conducted in pregnant (day 10) rats. It was found that prolactin maintained pregnancy in 90% of the test animals. HCG though found to maintain pregnancy in 70% of the PGF2a-treated pregnants, the fetal survival rate was, however, recorded to be 62% against the control value of 98%. Conversely, prolactin replacement maintained the fetal survival rate as high as 93.4%. Moreover, the growth of the fetuses, placentae and corpus luteum in the pregnants having prolactin in conjunction with PGF2a was also found to be greater compared to the animals which had a combined regimen of PGF2a and HCG, but identical to controls. On the other hand, similar combined regimen when applied to hysterectomized pregnant rats, it was observed that though the vaginal diestrus was maintained by the prolactin or HCG in the presence of PGF2a, the prolactin regimen was found to be superb compared to HCG in the maintenance of luteal weight and functional activity. It was concluded that the antifertility effect of PGF2a in the rat is primarily the consequence of luteolysis and prolactin seems to be a much more appropriate hormonal replacement compared to HCG, a long-acting LH, in antagonizing the luteolytic property of PGF2a.", "PMID": 973002}
{"id": "pubmed23n0032_9124", "title": "Jugular levels of 13, 14-dihydro-15-keto-prostaglandin F and progesterone around luteolysis and early pregnancy in the ewe.", "content": "Six non-pregnant ewes at day 12 of the estrous cycle each had a day-12 embryo transferred into the uterine horn ipsilateral to the corpus luteum, and 4 non-pregnant ewes at day 13 each had a day-13 embryo similarly transferred. Four control ewes, 2 at day 12 and 2 at day 13 received sheep serum into the uterine horn ipsilateral to the corpus luteum. Jugular blood samples were taken at 2-hourly intervals for 3 days post-surgery, then twice-daily for a further 4 days, and the plasma radioimmunoassayed for progesterone and 13, 14-dihydro-15-keto-prostaglandin F. All control ewes exhibited estrus within the expected time range and pulsatile peaks of 13, 14-dihydro-15-keto-prostaglandin F occurred coincident with declining progesterone levels. With one exception, the recipient owes had prolonged cycles and those ewes found pregnant at necropsy, 30 days after transfer, showed no progesterone decline and no pulsatile peaks of prostaglandin during days 12 to 16 after estrus. These observations suggest that the presence of the embryo at a critical stage after mating suppresses the release of uterine prostaglandin F2alpha.", "contents": "Jugular levels of 13, 14-dihydro-15-keto-prostaglandin F and progesterone around luteolysis and early pregnancy in the ewe. Six non-pregnant ewes at day 12 of the estrous cycle each had a day-12 embryo transferred into the uterine horn ipsilateral to the corpus luteum, and 4 non-pregnant ewes at day 13 each had a day-13 embryo similarly transferred. Four control ewes, 2 at day 12 and 2 at day 13 received sheep serum into the uterine horn ipsilateral to the corpus luteum. Jugular blood samples were taken at 2-hourly intervals for 3 days post-surgery, then twice-daily for a further 4 days, and the plasma radioimmunoassayed for progesterone and 13, 14-dihydro-15-keto-prostaglandin F. All control ewes exhibited estrus within the expected time range and pulsatile peaks of 13, 14-dihydro-15-keto-prostaglandin F occurred coincident with declining progesterone levels. With one exception, the recipient owes had prolonged cycles and those ewes found pregnant at necropsy, 30 days after transfer, showed no progesterone decline and no pulsatile peaks of prostaglandin during days 12 to 16 after estrus. These observations suggest that the presence of the embryo at a critical stage after mating suppresses the release of uterine prostaglandin F2alpha.", "PMID": 973003}
{"id": "pubmed23n0032_9125", "title": "Labor induction with prostaglandin F2alpha. Influence on psychomotor evolution of the child in the first 30 months.", "content": "The long-term effects of elective induction of labor at term by amniotomy and the intravenous infusion of prostaglandin F2alpha were studied in 40 children. No untoward effect of this procedure on the psychomotor development of the children during the first 30 months could be demonstrated.", "contents": "Labor induction with prostaglandin F2alpha. Influence on psychomotor evolution of the child in the first 30 months. The long-term effects of elective induction of labor at term by amniotomy and the intravenous infusion of prostaglandin F2alpha were studied in 40 children. No untoward effect of this procedure on the psychomotor development of the children during the first 30 months could be demonstrated.", "PMID": 973004}
{"id": "pubmed23n0032_9126", "title": "A possible role of prostaglandins in the regulation of ovum transport and oviposition in the domestic hen?", "content": "Prostaglandin (PG) F2 alpha provoked a contraction of longitudinal tissue strips from the uterine, vaginal and isthmus regions of the chicken oviduct; no response was induced in longitudinal tissue strips from the magnum and infundibulum. PGF2 induced a contraction of circular and longitudinal uterine strips and of circular stips from the isthmus and infundibulum. With both circular and longitudinal vaginal strips a relaxation was obtained. A dose-dependent response was observed with circular magnum strips: contraction with low doses and relaxation at higher doses. No response was obtained with longitudinal tissue strips from the isthmus, magnum and infundibulum. The possible influence of both prostaglandins in the mechanism of ovum transport and oviposition in the domestic hen is discussed.", "contents": "A possible role of prostaglandins in the regulation of ovum transport and oviposition in the domestic hen? Prostaglandin (PG) F2 alpha provoked a contraction of longitudinal tissue strips from the uterine, vaginal and isthmus regions of the chicken oviduct; no response was induced in longitudinal tissue strips from the magnum and infundibulum. PGF2 induced a contraction of circular and longitudinal uterine strips and of circular stips from the isthmus and infundibulum. With both circular and longitudinal vaginal strips a relaxation was obtained. A dose-dependent response was observed with circular magnum strips: contraction with low doses and relaxation at higher doses. No response was obtained with longitudinal tissue strips from the isthmus, magnum and infundibulum. The possible influence of both prostaglandins in the mechanism of ovum transport and oviposition in the domestic hen is discussed.", "PMID": 973005}
{"id": "pubmed23n0032_9127", "title": "Abortifacient efficiency of 15 (S) 15-methyl-prostaglandin F2alpha-methyl ester administered vaginally during early pregnancy.", "content": "Forty early pregnancies (menses delay 13 - 27 days) were terminated by administering four vaginal suppositoreis each containing 1.0 or 1.5 mg of 15 (S) 15-methyl-prostaglandin F2alpha-methyl ester, one every third hour. In 14 cases serial measurementsof serum estradiol and progesterone were performed during and after therapy. Uterine contractions and bleeding started 1 - 17 hours after administration of the first suppository. Abortion was complete after one week in five women (13%), and after two weeks in 30 (75%). A curettage was performed on eight women, residual placental fragments were found in seven and pregnancy continued in one woman. Mild diarrhoea (65%) and vomiting (40%) were the major side-effects, despite premedication. Estradiol and progesterone levels fell progressively during the therapy. Self-administration of 4 or 6 mg of the methyl ester caused too low a rate of complete abortion for use in practice, but it may be a valuable and practical agent for preoperative dilation of the cervix.", "contents": "Abortifacient efficiency of 15 (S) 15-methyl-prostaglandin F2alpha-methyl ester administered vaginally during early pregnancy. Forty early pregnancies (menses delay 13 - 27 days) were terminated by administering four vaginal suppositoreis each containing 1.0 or 1.5 mg of 15 (S) 15-methyl-prostaglandin F2alpha-methyl ester, one every third hour. In 14 cases serial measurementsof serum estradiol and progesterone were performed during and after therapy. Uterine contractions and bleeding started 1 - 17 hours after administration of the first suppository. Abortion was complete after one week in five women (13%), and after two weeks in 30 (75%). A curettage was performed on eight women, residual placental fragments were found in seven and pregnancy continued in one woman. Mild diarrhoea (65%) and vomiting (40%) were the major side-effects, despite premedication. Estradiol and progesterone levels fell progressively during the therapy. Self-administration of 4 or 6 mg of the methyl ester caused too low a rate of complete abortion for use in practice, but it may be a valuable and practical agent for preoperative dilation of the cervix.", "PMID": 973006}
{"id": "pubmed23n0032_9128", "title": "Use of combination prostaglandin F2alpha and hypertonic saline for midtrimester abortion.", "content": "Midtrimester abortion was induced by intramniotic prostaglandin F2alpha in combination with hypertonic saline alone in 102 others. Abortion time and duration of hosptial stay were shorter with the prostaglandin-saline combination.", "contents": "Use of combination prostaglandin F2alpha and hypertonic saline for midtrimester abortion. Midtrimester abortion was induced by intramniotic prostaglandin F2alpha in combination with hypertonic saline alone in 102 others. Abortion time and duration of hosptial stay were shorter with the prostaglandin-saline combination.", "PMID": 973007}
{"id": "pubmed23n0032_9129", "title": "Induced abortion in the 8-9th week of pregnancy with vaginally administered 15-methyl PGF2alpha methyl ester.", "content": "15(S)15-methyl PGF2alpha methyl ester was self-administered vaginally to terminate pregnancy in 42 women in the 8-9th week of gestation. Ten patients received a total of 6 mg of the compound over 15 hours (Group I) while the remaining 32 patients received 5.5 mg of the prostaglandin compound during a shorter period of time or 9 hours (Group II). If parts of the conceptus were expelled during treatment, surgical intervention was excluded. All patients were followed closely after treatment with repeated serum HCG assays and clinical examinations. All patients in Group II and eight out of ten patients in Group I aborted following treatment. In 33 of the 42 patients, the serum HCG levels and the clinical course following the expulsion of the conceptus indicated that abortion was complete. Gastro-intestinal side effects were minimal if anti-diarrheic agents were given prophylactically. The incidence of uterine pain was variable but could in most cases be controlled by oral or rectal administration of analgetics. The results of this study suggest that the use of this compound for termination of pregnancy may be safely extended through the 9th week of gestation and in certain cases be an alternative to the normal operative procedure.", "contents": "Induced abortion in the 8-9th week of pregnancy with vaginally administered 15-methyl PGF2alpha methyl ester. 15(S)15-methyl PGF2alpha methyl ester was self-administered vaginally to terminate pregnancy in 42 women in the 8-9th week of gestation. Ten patients received a total of 6 mg of the compound over 15 hours (Group I) while the remaining 32 patients received 5.5 mg of the prostaglandin compound during a shorter period of time or 9 hours (Group II). If parts of the conceptus were expelled during treatment, surgical intervention was excluded. All patients were followed closely after treatment with repeated serum HCG assays and clinical examinations. All patients in Group II and eight out of ten patients in Group I aborted following treatment. In 33 of the 42 patients, the serum HCG levels and the clinical course following the expulsion of the conceptus indicated that abortion was complete. Gastro-intestinal side effects were minimal if anti-diarrheic agents were given prophylactically. The incidence of uterine pain was variable but could in most cases be controlled by oral or rectal administration of analgetics. The results of this study suggest that the use of this compound for termination of pregnancy may be safely extended through the 9th week of gestation and in certain cases be an alternative to the normal operative procedure.", "PMID": 973008}
{"id": "pubmed23n0032_9130", "title": "Uneven ventilation in smokers.", "content": "A body plethysmographic technique designed to detect small degrees of uneven ventilation in the lungs is presented. The technique is based on the same theoretical model as the frequency dependence of compliance. It has the advantage of requiring little time, no cooperation by the subject and no computation. Signs of uneven ventilation were found in 3 of the 42 normal controls, in 6 of 51 light smokers and in 66 of 103 heavy smokers. The data are compared with measurements of closing volume, of the slope of phase III of the single breath nitrogen washout and of maximal mid-expiratory flow rate.", "contents": "Uneven ventilation in smokers. A body plethysmographic technique designed to detect small degrees of uneven ventilation in the lungs is presented. The technique is based on the same theoretical model as the frequency dependence of compliance. It has the advantage of requiring little time, no cooperation by the subject and no computation. Signs of uneven ventilation were found in 3 of the 42 normal controls, in 6 of 51 light smokers and in 66 of 103 heavy smokers. The data are compared with measurements of closing volume, of the slope of phase III of the single breath nitrogen washout and of maximal mid-expiratory flow rate.", "PMID": 973047}
{"id": "pubmed23n0032_9131", "title": "Frequency dependence of regional lung clearance of 133Xe in normal men.", "content": "We measured the one-half time of 133Xe washout from the whole lung and from 9 horizontal lung regions in 6 normal men during normal breathing and at 50 breaths/min. The diaphragm was located with a contour map prior to the selection of the 9 horizontal regions. Artifacts caused by the tissue solubility of 133Xe were reduced by expressing regional clearance relative to whole lung clearance. During normal breathing there was a progressive increase in the relative clearance of 133Xe from the lung apex to the base when each region was compared to the lung as a whole. At 50 breaths/min, relative clearance increased from the lung apex only for about 18.5 to 20 cm. At that point, the relative clearance rate of the horizontal regions began to fall and at the lung base was slower than that of the whole lung. These results are consistent with the concept that dependent lung regions have longer time constants than regions at the top of the lung.", "contents": "Frequency dependence of regional lung clearance of 133Xe in normal men. We measured the one-half time of 133Xe washout from the whole lung and from 9 horizontal lung regions in 6 normal men during normal breathing and at 50 breaths/min. The diaphragm was located with a contour map prior to the selection of the 9 horizontal regions. Artifacts caused by the tissue solubility of 133Xe were reduced by expressing regional clearance relative to whole lung clearance. During normal breathing there was a progressive increase in the relative clearance of 133Xe from the lung apex to the base when each region was compared to the lung as a whole. At 50 breaths/min, relative clearance increased from the lung apex only for about 18.5 to 20 cm. At that point, the relative clearance rate of the horizontal regions began to fall and at the lung base was slower than that of the whole lung. These results are consistent with the concept that dependent lung regions have longer time constants than regions at the top of the lung.", "PMID": 973048}
{"id": "pubmed23n0032_9132", "title": "Effects of simulated altitude on O2 transport in dogs.", "content": "The effects of exposure to simulated altitude on skeletal muscle and on the cardiocirculatory system rhave been studied on awake, conscious, dogs (16-39 kg) at rest and during moderate treadmill exercise at 15% incline. Measurements were obtained in Denver at a PB of 635 mm Hg and after 3 weeks of continuous exposure to a PB of 435 mm Hg using a hypobaric chamber. At both levels of PB the dogs showed marked panting, associated with pulmonary hyperventilation and low PaCO2's (28 mm Hg at 635 and 17 mm Hg at 435 mm Hg). Resting VO2's were high (12.4 and 11.4 ml/min-kg) reflecting the high respiratory outputs. [Hb] and Hct increased significantly after 3 weeks at 435 mm Hg with a 4 mm Hg decrease in P50. Q, HR, SV and systemic blood pressures were normal in both normoxic and hypoxic animals, at rest as well as during exercise. Arterial and mixed venous PO2's decreased in the hypoxic dogs (82 to 56 and 42 to 36 mm Hg, respectively) but arterial O2 content, arterial-venous O2 content difference and systemic O2 transport remained unchanged. Capillary density in the sternothyroid muscle doubled (617 to 1245 cap/mm2) after 3 weeks at a PB of 435, whereas the average muscle fiber diameter decreased about 30%. Calculations indicate that the PO2 in the 'lethal corner' of the muscle fiber is relatively higher in the hypoxic animal because of the decrease in fiber size and intercapillary distances.", "contents": "Effects of simulated altitude on O2 transport in dogs. The effects of exposure to simulated altitude on skeletal muscle and on the cardiocirculatory system rhave been studied on awake, conscious, dogs (16-39 kg) at rest and during moderate treadmill exercise at 15% incline. Measurements were obtained in Denver at a PB of 635 mm Hg and after 3 weeks of continuous exposure to a PB of 435 mm Hg using a hypobaric chamber. At both levels of PB the dogs showed marked panting, associated with pulmonary hyperventilation and low PaCO2's (28 mm Hg at 635 and 17 mm Hg at 435 mm Hg). Resting VO2's were high (12.4 and 11.4 ml/min-kg) reflecting the high respiratory outputs. [Hb] and Hct increased significantly after 3 weeks at 435 mm Hg with a 4 mm Hg decrease in P50. Q, HR, SV and systemic blood pressures were normal in both normoxic and hypoxic animals, at rest as well as during exercise. Arterial and mixed venous PO2's decreased in the hypoxic dogs (82 to 56 and 42 to 36 mm Hg, respectively) but arterial O2 content, arterial-venous O2 content difference and systemic O2 transport remained unchanged. Capillary density in the sternothyroid muscle doubled (617 to 1245 cap/mm2) after 3 weeks at a PB of 435, whereas the average muscle fiber diameter decreased about 30%. Calculations indicate that the PO2 in the 'lethal corner' of the muscle fiber is relatively higher in the hypoxic animal because of the decrease in fiber size and intercapillary distances.", "PMID": 973049}
{"id": "pubmed23n0032_9133", "title": "Respiratory properties of blood in awake and estivating lungfish, Protopterus amphibius.", "content": "Blood respiratory properties have been studied in awake and estivating African lungfish, Protopterus amphibius. Fish had been estivating 28-30 months when blood was sampled. Hematocrit, O2 capacity and blood hemoglobin concentration increased by about 50% during estivation. Red cell Hb concentration (MCHC) was unaltered. Total blood concentration of nucleoside triphosphates (NTP) was reduced 50% in estivation. Thin-layer chromatography showed that the change in NTP resulted from a GTP/Hb (guanosine triphosphate) reduction to 20% of the value in awake fish. ATP (adenosine triphosphate) concentration remained largely unaltered. GTP/Hb changes were accompanied by marked changes in O2 affinity. The P50 value in blood from awake fish was 33 mm Hg at pH 7.5 compared to 9 mm Hg for the estivating fish. The n-value changed insignificantly. An increased Bohr effect was present in estivating fish, but its importance will depend on circulating pH in the two states. Standard bicarbonate ranged from a low of 8.6 mMol-L-1 (P plasma) at pH 7.5 in an awake fish to 49.6 mMol-L-1 (P) in an estivating fish. CO2 dissociation curves showed a variable Haldane effect having its highest value in the in vivo range of PCO2. The striking increase in O2-Hb affinity during estivation is regarded as an adaptation to a reduced alveolar O2 availability associated with estivation. Altered mechanics of pulmonary ventilation and reduced ambient O2 availability caused by the subterranean habitat may have been selection pressures for the development of the high O2 availability caused by the subterranean habitat may have been selection pressures for the development of the high O2 affinity. The mechanism behind it rests with the recorded change in erythrocytic concentration of GTP.", "contents": "Respiratory properties of blood in awake and estivating lungfish, Protopterus amphibius. Blood respiratory properties have been studied in awake and estivating African lungfish, Protopterus amphibius. Fish had been estivating 28-30 months when blood was sampled. Hematocrit, O2 capacity and blood hemoglobin concentration increased by about 50% during estivation. Red cell Hb concentration (MCHC) was unaltered. Total blood concentration of nucleoside triphosphates (NTP) was reduced 50% in estivation. Thin-layer chromatography showed that the change in NTP resulted from a GTP/Hb (guanosine triphosphate) reduction to 20% of the value in awake fish. ATP (adenosine triphosphate) concentration remained largely unaltered. GTP/Hb changes were accompanied by marked changes in O2 affinity. The P50 value in blood from awake fish was 33 mm Hg at pH 7.5 compared to 9 mm Hg for the estivating fish. The n-value changed insignificantly. An increased Bohr effect was present in estivating fish, but its importance will depend on circulating pH in the two states. Standard bicarbonate ranged from a low of 8.6 mMol-L-1 (P plasma) at pH 7.5 in an awake fish to 49.6 mMol-L-1 (P) in an estivating fish. CO2 dissociation curves showed a variable Haldane effect having its highest value in the in vivo range of PCO2. The striking increase in O2-Hb affinity during estivation is regarded as an adaptation to a reduced alveolar O2 availability associated with estivation. Altered mechanics of pulmonary ventilation and reduced ambient O2 availability caused by the subterranean habitat may have been selection pressures for the development of the high O2 availability caused by the subterranean habitat may have been selection pressures for the development of the high O2 affinity. The mechanism behind it rests with the recorded change in erythrocytic concentration of GTP.", "PMID": 973050}
{"id": "pubmed23n0032_9134", "title": "Study of CO2 sensitive vagal afferents in the cat lung.", "content": "Single unit activity was recorded in the cat vagus in order to detect possible receptors firing in response to changing lung CO2 concentration. The cats were ventilated at a high rate (60-120 breaths per min) and inspired CO2 concentration was altered between 0 and 8% in a step-like fashion, each phase consisting of about 10 breaths. Thus the effects of changing intrapulmonary CO2 concentration could be differentiated from the effects of stretch of lung tissue. Activity was recorded in 7 cats from 120 units firing in phase with ventilation. Many receptors showed some CO2 sensitivity, but no fiber was found discharging in response to CO2 exclusively. The results provide no evidence for the occurence of specific CO2 receptors in the feline lung with vagal afferents functionally similar to those reported for the avian lung.", "contents": "Study of CO2 sensitive vagal afferents in the cat lung. Single unit activity was recorded in the cat vagus in order to detect possible receptors firing in response to changing lung CO2 concentration. The cats were ventilated at a high rate (60-120 breaths per min) and inspired CO2 concentration was altered between 0 and 8% in a step-like fashion, each phase consisting of about 10 breaths. Thus the effects of changing intrapulmonary CO2 concentration could be differentiated from the effects of stretch of lung tissue. Activity was recorded in 7 cats from 120 units firing in phase with ventilation. Many receptors showed some CO2 sensitivity, but no fiber was found discharging in response to CO2 exclusively. The results provide no evidence for the occurence of specific CO2 receptors in the feline lung with vagal afferents functionally similar to those reported for the avian lung.", "PMID": 973051}
{"id": "pubmed23n0032_9135", "title": "Respiratory frequency control during hypercapnia in vagotomized, anesthetized cats.", "content": "There is a small, but significant, increase in frequency during hypercapnia in vagotomized, anesthetized animals, indicating involvement of an extravagal mechanism in the response. The intent of this study was to determine the source of this second mechanism regulating frequency during hypercapnia. Experiments were performed on 22 vagotomized, anesthetized (Dial) cats. Frequency (f), inspiratory time (ti) and expiratory time (te) responses to CO2 were monitored before and after sectioning of afferent nerves from the carotid bodies (carotid sinus nerve section), chest wall (dorsal rhizotomies, T1-T12) and diaphragm (dorsal rhizotomies. C4-C7). Most vagotomized animals responded to 6% CO2 with an increased frequency, decreased ti and no consistent change in te. The responses to CO2 were essentially unaltered following chest wall and diaphragm deafferentation. Sodium cyanide stimulation of the carotid bodies produced similar respiratory pattern changes as CO2; furthermore, the f and ti changes with CO2 were still present following carotid body deafferentiation. The results of this study suggest that: (1) afferents from chest wall and diaphragm mechanoreceptors are not responsible for the vagal-like effects on ti and f during hypercapnia, (2) afferents from lung mechanoreceptors, via the vagus nerves, are the only inputs from respiratory mechanoreceptors causing an increased f during hypercapnia, (3) the extravagal mechanism responsible for the decreased ti and increased f during hypercapnia is inherent to the medullary-pontine rhythm generator, and (4) input from the chemoreceptors can elicit the response.", "contents": "Respiratory frequency control during hypercapnia in vagotomized, anesthetized cats. There is a small, but significant, increase in frequency during hypercapnia in vagotomized, anesthetized animals, indicating involvement of an extravagal mechanism in the response. The intent of this study was to determine the source of this second mechanism regulating frequency during hypercapnia. Experiments were performed on 22 vagotomized, anesthetized (Dial) cats. Frequency (f), inspiratory time (ti) and expiratory time (te) responses to CO2 were monitored before and after sectioning of afferent nerves from the carotid bodies (carotid sinus nerve section), chest wall (dorsal rhizotomies, T1-T12) and diaphragm (dorsal rhizotomies. C4-C7). Most vagotomized animals responded to 6% CO2 with an increased frequency, decreased ti and no consistent change in te. The responses to CO2 were essentially unaltered following chest wall and diaphragm deafferentation. Sodium cyanide stimulation of the carotid bodies produced similar respiratory pattern changes as CO2; furthermore, the f and ti changes with CO2 were still present following carotid body deafferentiation. The results of this study suggest that: (1) afferents from chest wall and diaphragm mechanoreceptors are not responsible for the vagal-like effects on ti and f during hypercapnia, (2) afferents from lung mechanoreceptors, via the vagus nerves, are the only inputs from respiratory mechanoreceptors causing an increased f during hypercapnia, (3) the extravagal mechanism responsible for the decreased ti and increased f during hypercapnia is inherent to the medullary-pontine rhythm generator, and (4) input from the chemoreceptors can elicit the response.", "PMID": 973052}
{"id": "pubmed23n0032_9136", "title": "Ventilation, gas exchange and metabolic scaling of a sea turtle.", "content": "Ventilation of green turtles (Chelonia mydas) was affected by the position in which the animal was placed: supine animals breathed slowly 0.07 breaths/min) and deeply (8.0 L/breath); prone animals breathed more rapidly (0.43 breaths/min) and more shallowly (3.5 L/breath). From the respiratory exchange ratio and other indicators it appears that green turtles hyperventilate during exercise and hypoventilate during recovery. O2 consumption of the resting sea turtle (0.024 L-kg-1-h-1) is similar to that of other large turtles. Maximal O2 consumption (0.25L-kg-1-h-1) is greater than that of other large turtles. Minimal O2 consumption scaled in proportion to the -0.17 power of the body mass of green turtles over the range of 0.030 to 141.5 kg. The maximal O2 consumption scaled in proportion to the -0.06 power of body mass for the same range of body masses.", "contents": "Ventilation, gas exchange and metabolic scaling of a sea turtle. Ventilation of green turtles (Chelonia mydas) was affected by the position in which the animal was placed: supine animals breathed slowly 0.07 breaths/min) and deeply (8.0 L/breath); prone animals breathed more rapidly (0.43 breaths/min) and more shallowly (3.5 L/breath). From the respiratory exchange ratio and other indicators it appears that green turtles hyperventilate during exercise and hypoventilate during recovery. O2 consumption of the resting sea turtle (0.024 L-kg-1-h-1) is similar to that of other large turtles. Maximal O2 consumption (0.25L-kg-1-h-1) is greater than that of other large turtles. Minimal O2 consumption scaled in proportion to the -0.17 power of the body mass of green turtles over the range of 0.030 to 141.5 kg. The maximal O2 consumption scaled in proportion to the -0.06 power of body mass for the same range of body masses.", "PMID": 973053}
{"id": "pubmed23n0032_9137", "title": "[Familial olivo-ponto-cerebellar atrophy with myoclonus. Limits of cerebellar myoclonic dyssynergia (Ramsay-Hunt syndrome)].", "content": "The case is described of a woman of 26 suffering (like her mother, a brother and a sister) from a progressively degenerating cerebellar syndrome, at first considered to be hereditary cerebellar ataxia, but which, after action myoclonus appeared, was diagnosed as dyssynergia cerebellaris myoclonica (D.C.M.). Anatomical verification however revealed a syndrome of olivo-ponto-cerebellar atrophy comprising massive demyelinisation of the white matter of the cerebellum and of the cerebellopontine fibres; atrophy of the pontine nuclei; the cerebellar cortex itself was severely affected; moderate nigral lesions; marked spinal lesions of the cerebellospinal fasciculi and of the posterior columns; lesions of the anterior horns and of the bulbar nuclei; cortical chromatolysis. The fact that the dentate system remained unaffected, also noted in some cases of olivo-ponto-cerebellar atrophy with myoclonus, whilst in a number of other cases the lesion remains clinically silent, emphasises the difficulty in establishing an accurate correlation between myoclonus and dentate nucleus. Discussion of the nosological limits of D.C.M.: confirmed cases generally displayed lesions of the dentate system and hereditary degenerative spino-cerebellar lesions. The same clinical symptoms can be observed in cases which do not come under this classification--or even under that of degenerative conditions of the cerebellar system--and D.C.M. appears to be only a syndrome, the Ramsay-Hunt syndrome.", "contents": "[Familial olivo-ponto-cerebellar atrophy with myoclonus. Limits of cerebellar myoclonic dyssynergia (Ramsay-Hunt syndrome)]. The case is described of a woman of 26 suffering (like her mother, a brother and a sister) from a progressively degenerating cerebellar syndrome, at first considered to be hereditary cerebellar ataxia, but which, after action myoclonus appeared, was diagnosed as dyssynergia cerebellaris myoclonica (D.C.M.). Anatomical verification however revealed a syndrome of olivo-ponto-cerebellar atrophy comprising massive demyelinisation of the white matter of the cerebellum and of the cerebellopontine fibres; atrophy of the pontine nuclei; the cerebellar cortex itself was severely affected; moderate nigral lesions; marked spinal lesions of the cerebellospinal fasciculi and of the posterior columns; lesions of the anterior horns and of the bulbar nuclei; cortical chromatolysis. The fact that the dentate system remained unaffected, also noted in some cases of olivo-ponto-cerebellar atrophy with myoclonus, whilst in a number of other cases the lesion remains clinically silent, emphasises the difficulty in establishing an accurate correlation between myoclonus and dentate nucleus. Discussion of the nosological limits of D.C.M.: confirmed cases generally displayed lesions of the dentate system and hereditary degenerative spino-cerebellar lesions. The same clinical symptoms can be observed in cases which do not come under this classification--or even under that of degenerative conditions of the cerebellar system--and D.C.M. appears to be only a syndrome, the Ramsay-Hunt syndrome.", "PMID": 973068}
{"id": "pubmed23n0032_9138", "title": "[Striato-nigral degeneration. Apropos of a clinical, therapeutic, and anatomic study of 2 cases].", "content": "The authors report two anatomo-clinical cases of striato-nigral degeneration with trial of L-Dopa; one of them included an olivoponto-cerebellar degeneration. The features of this degeneration are compared with the literature and related to heredo-degenerative diseases of the nervous system.", "contents": "[Striato-nigral degeneration. Apropos of a clinical, therapeutic, and anatomic study of 2 cases]. The authors report two anatomo-clinical cases of striato-nigral degeneration with trial of L-Dopa; one of them included an olivoponto-cerebellar degeneration. The features of this degeneration are compared with the literature and related to heredo-degenerative diseases of the nervous system.", "PMID": 973069}
{"id": "pubmed23n0032_9139", "title": "[Loss of voluntary control and retention of automatic reflex in certain muscles innervated by cranial nerves in 2 cases of amyotrophic lateral sclerosis].", "content": "The authors present two clinical observations of amyotrophic lateral sclerosis (A.L.S.) in which a loss of voluntary control was associated with retention of automatic and reflex activity of muscles innervated by cranial nerves. In the first case the face and swallowing were affected, in the second the movements of the eyes. These phenomena are exceptional in A.L.S.", "contents": "[Loss of voluntary control and retention of automatic reflex in certain muscles innervated by cranial nerves in 2 cases of amyotrophic lateral sclerosis]. The authors present two clinical observations of amyotrophic lateral sclerosis (A.L.S.) in which a loss of voluntary control was associated with retention of automatic and reflex activity of muscles innervated by cranial nerves. In the first case the face and swallowing were affected, in the second the movements of the eyes. These phenomena are exceptional in A.L.S.", "PMID": 973070}
{"id": "pubmed23n0032_9140", "title": "[Major histocompatibility system in multiple sclerosis].", "content": "The comparison of Histocompatibility Testing in 82 MS Patients and 368 controls is presented. The increase in HL-A7 and decrease in HL-A12 are confirmed. A significant increase in HL-A8 is reported. Mixed Lymphocyte Reaction confirms the LD7a increase (19 out of 24 Multiple Sclerosis patients tested); 100% of the patients bearing the HL-A7 determinant are found to be LD7a. The presence of specific Immune Response genes in Multiple Sclerosis is discussed.", "contents": "[Major histocompatibility system in multiple sclerosis]. The comparison of Histocompatibility Testing in 82 MS Patients and 368 controls is presented. The increase in HL-A7 and decrease in HL-A12 are confirmed. A significant increase in HL-A8 is reported. Mixed Lymphocyte Reaction confirms the LD7a increase (19 out of 24 Multiple Sclerosis patients tested); 100% of the patients bearing the HL-A7 determinant are found to be LD7a. The presence of specific Immune Response genes in Multiple Sclerosis is discussed.", "PMID": 973072}
{"id": "pubmed23n0032_9141", "title": "[The Atri system. Immunogenetic lymphocytic system associated with ABO and ABH secretory systems].", "content": "The antigen Atri can be detected on the lymphocytes of 5.19% of ABH Secretor A individuals. The Atri substance is present in the plasma of the same individuals and can be fixed on group O erythrocytes. It is also found in the saliva of 30/32 A individuals not secreting substance A. Its existence in the saliva of A Secretor individuals, however, cannot be proved due to the presence of both the A and Atri antigens. The Atri substance is neutralized by the soluble substance A extracted from the gastric mucous of a pig. Yet the A and Atri antigens can be shown to be distinct on lymphocytes by redistribution of the corresponding sites and blocking of the A sites by an immune anti-A. On a genetic level, the Atri specificity might belong to a lymphocyte system comprising three alleles: Atri, RB and W100. These three specificities have the common characteristic of being expressed on a certain percentage of the lymphocytes of group A individuals. This system, provisionally named Atri, is functionally associated with the ABO system and secretor system. Nevertheless, the fact that in several families the Atri antigen is not expressed by the parents shows that its expression on lymphocytes requires the intervention of at least one gene in addition to the A and Se genes.", "contents": "[The Atri system. Immunogenetic lymphocytic system associated with ABO and ABH secretory systems]. The antigen Atri can be detected on the lymphocytes of 5.19% of ABH Secretor A individuals. The Atri substance is present in the plasma of the same individuals and can be fixed on group O erythrocytes. It is also found in the saliva of 30/32 A individuals not secreting substance A. Its existence in the saliva of A Secretor individuals, however, cannot be proved due to the presence of both the A and Atri antigens. The Atri substance is neutralized by the soluble substance A extracted from the gastric mucous of a pig. Yet the A and Atri antigens can be shown to be distinct on lymphocytes by redistribution of the corresponding sites and blocking of the A sites by an immune anti-A. On a genetic level, the Atri specificity might belong to a lymphocyte system comprising three alleles: Atri, RB and W100. These three specificities have the common characteristic of being expressed on a certain percentage of the lymphocytes of group A individuals. This system, provisionally named Atri, is functionally associated with the ABO system and secretor system. Nevertheless, the fact that in several families the Atri antigen is not expressed by the parents shows that its expression on lymphocytes requires the intervention of at least one gene in addition to the A and Se genes.", "PMID": 973080}
{"id": "pubmed23n0032_9142", "title": "Typical results of computer-olfactometry.", "content": "After pulsed peripheral olfactory impulses it is possible, under suitable testing conditions, for specific sensory potentials to be read from the surface of the skull and, after repetitions, added up (olfactorially evoked cortical potentials). Typically, there developes a so-called twin-potential containing the equivalents of the electrical activites of the nervus trigeminus and the nervus olfactorius in two peaks of the cortex equivalents. In an objective manner, in other words without intentional or vegetative influence by the test person, it is possible by the weakening or lack of one or the other or both part-potentials to obtain information about the functioning of the olfactory sense. Typical examples will be presented.", "contents": "Typical results of computer-olfactometry. After pulsed peripheral olfactory impulses it is possible, under suitable testing conditions, for specific sensory potentials to be read from the surface of the skull and, after repetitions, added up (olfactorially evoked cortical potentials). Typically, there developes a so-called twin-potential containing the equivalents of the electrical activites of the nervus trigeminus and the nervus olfactorius in two peaks of the cortex equivalents. In an objective manner, in other words without intentional or vegetative influence by the test person, it is possible by the weakening or lack of one or the other or both part-potentials to obtain information about the functioning of the olfactory sense. Typical examples will be presented.", "PMID": 973085}
{"id": "pubmed23n0032_9143", "title": "Experience with the new pneumatic nasal tampon in cases of critical nasal bleeding.", "content": "A pneumatic nasal tube is recommended to control serious bleeding in the nasal cavity and nasopharynx. This is easily administered and tightly seals the nasal cavity and nasopharynx. The tube allows reduced nasal respiration with sufficient sealing. It is available in three sizes and has been proven useful by the authors.", "contents": "Experience with the new pneumatic nasal tampon in cases of critical nasal bleeding. A pneumatic nasal tube is recommended to control serious bleeding in the nasal cavity and nasopharynx. This is easily administered and tightly seals the nasal cavity and nasopharynx. The tube allows reduced nasal respiration with sufficient sealing. It is available in three sizes and has been proven useful by the authors.", "PMID": 973086}
{"id": "pubmed23n0032_9144", "title": "[Basic principles of radioimmunoanalysis].", "content": "The authors review  the principal characteristics of radioimmunoassay (R.I.A.), in grounds of the so-called \"saturation analysis\" techniques, which are of increasing interest among the chemical laboratory procedures. They study each element of R.I.A. separatedly, and finally they make a brief display of the different applications of radioimmunoassay stressing its significance in the endocrinological field.", "contents": "[Basic principles of radioimmunoanalysis]. The authors review  the principal characteristics of radioimmunoassay (R.I.A.), in grounds of the so-called \"saturation analysis\" techniques, which are of increasing interest among the chemical laboratory procedures. They study each element of R.I.A. separatedly, and finally they make a brief display of the different applications of radioimmunoassay stressing its significance in the endocrinological field.", "PMID": 973081}
{"id": "pubmed23n0032_9145", "title": "Disordered gas exchange in the blood caused by nasal tamponade.", "content": "The authors have examined the pH, the pCO2, the pO2, and the oxygen saturation of the blood of patients upon whom endonasal surgery followed by tamponing of the nose had been performed. The gas values were examined on the second postoperative day and two days after the tampons had been removed. It was found that the blood pH remained within normal limits, whereas during the acute obstruction the pO2 values were diminished, the pCO2 values were increased, and the oxygen saturation of the blood was noticeably diminished. The authors recommend carefulness if the noses of patients with cardiorespiratory insufficiency are to be tamponed.", "contents": "Disordered gas exchange in the blood caused by nasal tamponade. The authors have examined the pH, the pCO2, the pO2, and the oxygen saturation of the blood of patients upon whom endonasal surgery followed by tamponing of the nose had been performed. The gas values were examined on the second postoperative day and two days after the tampons had been removed. It was found that the blood pH remained within normal limits, whereas during the acute obstruction the pO2 values were diminished, the pCO2 values were increased, and the oxygen saturation of the blood was noticeably diminished. The authors recommend carefulness if the noses of patients with cardiorespiratory insufficiency are to be tamponed.", "PMID": 973087}
{"id": "pubmed23n0032_9146", "title": "[Effects of adrenalectomy and cortisol administration on the concentration of free fatty acids, triglycerides and total lipids on the liver of rats].", "content": "The hepatic concentration of triglycerides, free fatty acids and total lipids was determined in four groups of rats: normals, adrenectomized and normal and adrenectomized previously treated with cortisol, 30 mg. In chronic adrenectomized state, total lipids and hepatic triglycerides are diminished, particularly the triglycerides. Adrenectomy does not appear to have significative effect on the free fatty acids. The administration of cortisol does not change those observations and, when given to normal animals, decreases the hepatic concentrations of free fatty acids, triglycerides and total lipids.", "contents": "[Effects of adrenalectomy and cortisol administration on the concentration of free fatty acids, triglycerides and total lipids on the liver of rats]. The hepatic concentration of triglycerides, free fatty acids and total lipids was determined in four groups of rats: normals, adrenectomized and normal and adrenectomized previously treated with cortisol, 30 mg. In chronic adrenectomized state, total lipids and hepatic triglycerides are diminished, particularly the triglycerides. Adrenectomy does not appear to have significative effect on the free fatty acids. The administration of cortisol does not change those observations and, when given to normal animals, decreases the hepatic concentrations of free fatty acids, triglycerides and total lipids.", "PMID": 973083}
{"id": "pubmed23n0032_9147", "title": "[Incorporation of 1-C14 palmitic acid into liver lipids of rats in different hormanal states].", "content": "Adrenalectomy and/or cortisol-induced variations in the in vivo and in vitro incorporation of 14C-palmitic acid 1 into the different fractions of rat liver lipids were studied. The different fatty acids incorporations follow analogous patterns in every hormonal state in vitro. Incorporation rate is faster in normal animals, but adrenalectomy or the administration of cortisol produces a marded delay. Cortisol does not modify the anomalism observed in the adrenoprival state. In vivo, the incorporation of palmitic acid into the different liver lipids--for the same hormonal state--does not follow the same pattern.", "contents": "[Incorporation of 1-C14 palmitic acid into liver lipids of rats in different hormanal states]. Adrenalectomy and/or cortisol-induced variations in the in vivo and in vitro incorporation of 14C-palmitic acid 1 into the different fractions of rat liver lipids were studied. The different fatty acids incorporations follow analogous patterns in every hormonal state in vitro. Incorporation rate is faster in normal animals, but adrenalectomy or the administration of cortisol produces a marded delay. Cortisol does not modify the anomalism observed in the adrenoprival state. In vivo, the incorporation of palmitic acid into the different liver lipids--for the same hormonal state--does not follow the same pattern.", "PMID": 973082}
{"id": "pubmed23n0032_9148", "title": "Endoscopic and histopathological observations of chronic maxillary sinusitis.", "content": "111 patients are studied in order to find possible relations between the endoscopic images and histopathological findings in the chronic maxillary sinusitis. The clasification of the different types of sinusitis is based on the aspect of the mucosa (normoplastic, hypoplastic, polypous, polypoido-polypous) and on the histological features (lymphoplasmocytic, eosinophilic, eosino-lymphoplasmocytic infiltration, low cellularity). The confrontation of the sinusoscopic and histopathological observations give interesting information, such as differenciation between stabilized and evolutive sinusitis, simple and complicated inflammation. This information facilitates our therapeutic choice.", "contents": "Endoscopic and histopathological observations of chronic maxillary sinusitis. 111 patients are studied in order to find possible relations between the endoscopic images and histopathological findings in the chronic maxillary sinusitis. The clasification of the different types of sinusitis is based on the aspect of the mucosa (normoplastic, hypoplastic, polypous, polypoido-polypous) and on the histological features (lymphoplasmocytic, eosinophilic, eosino-lymphoplasmocytic infiltration, low cellularity). The confrontation of the sinusoscopic and histopathological observations give interesting information, such as differenciation between stabilized and evolutive sinusitis, simple and complicated inflammation. This information facilitates our therapeutic choice.", "PMID": 973088}
{"id": "pubmed23n0032_9149", "title": "[Hormone dependence in functional thyroid cancers, survival after total thyroidectomy combined with radioactive iodine and thyroid extract therapy].", "content": "The authors reviewed current criteria on hormone-dependence in functional thyroid cancers, presenting data of compared disease evolution based on the casuistics of thyroid carcinomas from the files of the Fundaci\u00f3n Jim\u00e9nez D\u00edaz, with 5 and 10 year follow-up studies in some of the cases. They found a higher number of survivors among the patients with papilliferous carcinoma in both 5 and 10 year follow-up groups whom, aside total surgical removal of the gland and the administration of thyroid extracts, those who had thyroid residues left were given 100 mc of 131I. Among the patients with follicular adenocarcinoma, under the same comparative conditions, differences were not very clear in the 5 year follow-up group, while the 10 year follow-up group showed marked differences. The authors conclude that an adequate combination of the three therapeutic procedures may allow a higher survival rate.", "contents": "[Hormone dependence in functional thyroid cancers, survival after total thyroidectomy combined with radioactive iodine and thyroid extract therapy]. The authors reviewed current criteria on hormone-dependence in functional thyroid cancers, presenting data of compared disease evolution based on the casuistics of thyroid carcinomas from the files of the Fundaci\u00f3n Jim\u00e9nez D\u00edaz, with 5 and 10 year follow-up studies in some of the cases. They found a higher number of survivors among the patients with papilliferous carcinoma in both 5 and 10 year follow-up groups whom, aside total surgical removal of the gland and the administration of thyroid extracts, those who had thyroid residues left were given 100 mc of 131I. Among the patients with follicular adenocarcinoma, under the same comparative conditions, differences were not very clear in the 5 year follow-up group, while the 10 year follow-up group showed marked differences. The authors conclude that an adequate combination of the three therapeutic procedures may allow a higher survival rate.", "PMID": 973084}
{"id": "pubmed23n0032_9150", "title": "The correlation between permanent septal deformities and nasal trauma during birth.", "content": "Both the outer and inner nose as well as the position of the parietal bones of 972 patients at an age of 3 to 82, were examined giving evidence of the configuration of the infant's head at birth and thereby of the \"severity\" of the birth. In comparison with the sectio-patients the configuration of the infant's head in the birth canal reveals statistically recorded deformations of the outer and inner nose with an obstruction of the nasal breathing in 20% of the patients examined. The degree of the configuration has a definite influence on the degree of the nose deformation. The adjustment of the rotary direction of the infant's head in the 1st and 2nd vertex presentation determines the direction of the nose deformation. The demand of many authors for an immediate rhinological examination and corrections in the newborn child, for instance on the septum, to be performed still in the obstetrical department, must due to our own examinations be advocated.", "contents": "The correlation between permanent septal deformities and nasal trauma during birth. Both the outer and inner nose as well as the position of the parietal bones of 972 patients at an age of 3 to 82, were examined giving evidence of the configuration of the infant's head at birth and thereby of the \"severity\" of the birth. In comparison with the sectio-patients the configuration of the infant's head in the birth canal reveals statistically recorded deformations of the outer and inner nose with an obstruction of the nasal breathing in 20% of the patients examined. The degree of the configuration has a definite influence on the degree of the nose deformation. The adjustment of the rotary direction of the infant's head in the 1st and 2nd vertex presentation determines the direction of the nose deformation. The demand of many authors for an immediate rhinological examination and corrections in the newborn child, for instance on the septum, to be performed still in the obstetrical department, must due to our own examinations be advocated.", "PMID": 973090}
{"id": "pubmed23n0032_9151", "title": "Results of clinical olfactometric studies.", "content": "A modification of a flow olfactometer with a new application appartus, with which \"quasi-free\" nasal respiration allows the elimination of adaptation without a special testing room, subsequent results using this device to examine olfactory thresholds before and after septum operations, as well as reference to threshold increases in 57 post-operative cases of cheilognathopalatoschisis are reported. An esthesio-neuroblastoma as well as the deformity syndrome with cheilognathopalatoschisis and encephalodystrophy are used as examples for combined olfactory transmission and perception disorders. Studies of 55 smokers with primary neurosensory disorders demonstrated a threefold increase in the olfactory threshold and an up to 50% decrease \"fatique-time\". A mean acetone deviation factor of 1.93 was seen in 100 students from 20-27 years of age before and after eating. Correspondingly, after a substantial breakfast and lunch, the olfactory threshold attained its maximum daily value within 90 minutes, much more pronounced than after intake of 80 grams of glucose solution. In contrast to the literature, the olfactory threshold was seen to continuously increase, dependent on age. Studies of the perceptive and recognition threshold on 100 normal individuals and 28 patients with hyposmia exhibited with 3 sigma, a significant difference. In patients with hyposmia, the absolute values for the two threshold types vary greatly, however not their deviation factors. More importance should be attached to the sense of smell as the so-called lesser senses give us the greatest pleasures.", "contents": "Results of clinical olfactometric studies. A modification of a flow olfactometer with a new application appartus, with which \"quasi-free\" nasal respiration allows the elimination of adaptation without a special testing room, subsequent results using this device to examine olfactory thresholds before and after septum operations, as well as reference to threshold increases in 57 post-operative cases of cheilognathopalatoschisis are reported. An esthesio-neuroblastoma as well as the deformity syndrome with cheilognathopalatoschisis and encephalodystrophy are used as examples for combined olfactory transmission and perception disorders. Studies of 55 smokers with primary neurosensory disorders demonstrated a threefold increase in the olfactory threshold and an up to 50% decrease \"fatique-time\". A mean acetone deviation factor of 1.93 was seen in 100 students from 20-27 years of age before and after eating. Correspondingly, after a substantial breakfast and lunch, the olfactory threshold attained its maximum daily value within 90 minutes, much more pronounced than after intake of 80 grams of glucose solution. In contrast to the literature, the olfactory threshold was seen to continuously increase, dependent on age. Studies of the perceptive and recognition threshold on 100 normal individuals and 28 patients with hyposmia exhibited with 3 sigma, a significant difference. In patients with hyposmia, the absolute values for the two threshold types vary greatly, however not their deviation factors. More importance should be attached to the sense of smell as the so-called lesser senses give us the greatest pleasures.", "PMID": 973091}
{"id": "pubmed23n0032_9152", "title": "[Superior and inferior cavography as well as azygography and retroperitoneal venography: methods, indications and results (author's transl)].", "content": "The diagnostic spectrum for venography of the trunk is broad. During the traditional superior and inferior cavography, which should be used for clinical bone marrow functional blockage in connection with a confirmed tumor or for thrombosis, the combined retroperitoneal venography and cavography which we developed for clinical use as well as direct azygography provide valuable supplementary information which can be significant especially for preoperative questions. In the case of carcinoma of the esophagus or stomach, the question whether or not the tumor can be surgically removed or whether the tumor is already inoperable may be answered before surgery. In addition, direct azygography is suitable as a method to seach for lymph nodes metastases in carcinomas of the lung, stomach, kidney, adrenals and pancreas which cannot be ascertained via mediastinalscopy, and for systemic diseases. In addition to the prognostic evaluation of the tumor  and the additional preoperative evaluation of neighboring tumors, various bone diseases of the lumbar vertebrae, e.g., inflammatory, tumerous and traumatic changes in the spine, may be localized on the retroperitoneal venogram and cavogram. They can also be used successfully to seach for metastases or in the case of a dislocation of an intervertebral disk. The execution of the various methods is relatively simple and they may be performed on a patient capable of surgery.", "contents": "[Superior and inferior cavography as well as azygography and retroperitoneal venography: methods, indications and results (author's transl)]. The diagnostic spectrum for venography of the trunk is broad. During the traditional superior and inferior cavography, which should be used for clinical bone marrow functional blockage in connection with a confirmed tumor or for thrombosis, the combined retroperitoneal venography and cavography which we developed for clinical use as well as direct azygography provide valuable supplementary information which can be significant especially for preoperative questions. In the case of carcinoma of the esophagus or stomach, the question whether or not the tumor can be surgically removed or whether the tumor is already inoperable may be answered before surgery. In addition, direct azygography is suitable as a method to seach for lymph nodes metastases in carcinomas of the lung, stomach, kidney, adrenals and pancreas which cannot be ascertained via mediastinalscopy, and for systemic diseases. In addition to the prognostic evaluation of the tumor  and the additional preoperative evaluation of neighboring tumors, various bone diseases of the lumbar vertebrae, e.g., inflammatory, tumerous and traumatic changes in the spine, may be localized on the retroperitoneal venogram and cavogram. They can also be used successfully to seach for metastases or in the case of a dislocation of an intervertebral disk. The execution of the various methods is relatively simple and they may be performed on a patient capable of surgery.", "PMID": 973100}
{"id": "pubmed23n0032_9153", "title": "[Hyaline membrane disease: pulmonary changes and complications during ventilatory assistance in preterm infants (authors transl)].", "content": "The changing pattern of hyaline membrane disease and its iatrogenic complications during respiratory treatment are reviewed. The typical roentgenologic symptoms of pulmonary interstitial emphysema, pseudocyst, pneumomediastinum, pneumoperitoneum, pneumothorax, pneumopericardium, bronchopulmonary dysplasia and pulmonary heamorrhage are described and illustrated. Their relevance for clinical management is discussed.", "contents": "[Hyaline membrane disease: pulmonary changes and complications during ventilatory assistance in preterm infants (authors transl)]. The changing pattern of hyaline membrane disease and its iatrogenic complications during respiratory treatment are reviewed. The typical roentgenologic symptoms of pulmonary interstitial emphysema, pseudocyst, pneumomediastinum, pneumoperitoneum, pneumothorax, pneumopericardium, bronchopulmonary dysplasia and pulmonary heamorrhage are described and illustrated. Their relevance for clinical management is discussed.", "PMID": 973101}
{"id": "pubmed23n0032_9154", "title": "[The use of the liver-spleen-scan for the diagnosis of congenital Intrahepatic cysts of the bile duct (M. Caroli) (authors transl)].", "content": "After the short review of the pathogenesis, diagnosis and therapy for congenital intrahepatic cysts of the bile-duct (morbus Caroli), reference was made to the diagnostic significance of liver scintigraphy on the basis of two casuistic reports. Liver scintigraphy permits an exact localization of intrahepatic cysts as well as information regarding the course of the hepatopathy connected with morbus Caroli. In addition, 131-J-bromsulfon scintigraphy of the liver is a simple method for controlling the patency of the bile ducts. Our observations tend to indicate that preoperative and postoperative scintigraphic controls of the liver are practical for this disease.", "contents": "[The use of the liver-spleen-scan for the diagnosis of congenital Intrahepatic cysts of the bile duct (M. Caroli) (authors transl)]. After the short review of the pathogenesis, diagnosis and therapy for congenital intrahepatic cysts of the bile-duct (morbus Caroli), reference was made to the diagnostic significance of liver scintigraphy on the basis of two casuistic reports. Liver scintigraphy permits an exact localization of intrahepatic cysts as well as information regarding the course of the hepatopathy connected with morbus Caroli. In addition, 131-J-bromsulfon scintigraphy of the liver is a simple method for controlling the patency of the bile ducts. Our observations tend to indicate that preoperative and postoperative scintigraphic controls of the liver are practical for this disease.", "PMID": 973102}
{"id": "pubmed23n0032_9155", "title": "[Roentgen Diagnosis of injuries of the diaphragm in childhood (authors transl)].", "content": "Three cases of traumatic rupture of the diaphragm were presented. In terms of the roentgen examination, one can differentiate between certain and incertain diagnostic criteria. The differential diagnosis was briefly metioned; an optimal examination technique is often not possible due to the severity of the injuries.", "contents": "[Roentgen Diagnosis of injuries of the diaphragm in childhood (authors transl)]. Three cases of traumatic rupture of the diaphragm were presented. In terms of the roentgen examination, one can differentiate between certain and incertain diagnostic criteria. The differential diagnosis was briefly metioned; an optimal examination technique is often not possible due to the severity of the injuries.", "PMID": 973103}
{"id": "pubmed23n0032_9156", "title": "[New intensifier screens in pediatric radiology (authors transl)].", "content": "On the basis of the physical and clinical findings of this investigation, the characteristics of new screens made of rare earths were described. The possibilities of application for pediatric radiology and those advantages which can be achieved with the new screens were discussed. Possible technical difficulties were mentioned.", "contents": "[New intensifier screens in pediatric radiology (authors transl)]. On the basis of the physical and clinical findings of this investigation, the characteristics of new screens made of rare earths were described. The possibilities of application for pediatric radiology and those advantages which can be achieved with the new screens were discussed. Possible technical difficulties were mentioned.", "PMID": 973104}
{"id": "pubmed23n0032_9157", "title": "[Patient dosage load with the vidio amplifier technique in pediatric radiology (authors transl)].", "content": "The desired reduction of dosage with the indirect technique compared with the close up is only obtained if the vidio amplifier (i.e. 50 muR/picture) is adjusted exactly, if the input potential of 60 kV is not exceeded, particularly for infants, and no high kV grids are used. An adjustable diaphragm in front of the camera with the adjustment of the vidio amplifier and with the electron-optical changeover from, for example 9\" to 5\", would result in an additional reduction in dosage.", "contents": "[Patient dosage load with the vidio amplifier technique in pediatric radiology (authors transl)]. The desired reduction of dosage with the indirect technique compared with the close up is only obtained if the vidio amplifier (i.e. 50 muR/picture) is adjusted exactly, if the input potential of 60 kV is not exceeded, particularly for infants, and no high kV grids are used. An adjustable diaphragm in front of the camera with the adjustment of the vidio amplifier and with the electron-optical changeover from, for example 9\" to 5\", would result in an additional reduction in dosage.", "PMID": 973105}
{"id": "pubmed23n0032_9158", "title": "[A special gonad protector for comparative radiograms of the hips in infants (authors transl)].", "content": "A special, newly developed, gonad protector to be used for comparative radiograms of the hips in infants was introduced. This lead protector has a window which, due to its special shape, exposes only that portion of the skeleton which is important for the evaluation of dysplasia of the hip. In order to better protect the testes from scattered rays, a trough shaped lead plate is placed behind the scrotum.", "contents": "[A special gonad protector for comparative radiograms of the hips in infants (authors transl)]. A special, newly developed, gonad protector to be used for comparative radiograms of the hips in infants was introduced. This lead protector has a window which, due to its special shape, exposes only that portion of the skeleton which is important for the evaluation of dysplasia of the hip. In order to better protect the testes from scattered rays, a trough shaped lead plate is placed behind the scrotum.", "PMID": 973106}
{"id": "pubmed23n0032_9159", "title": "The antisteroid action on the pineal gland.", "content": "The study presents the data gathered along the years on the antisteroid action of the pineal gland. Thus, it was demonstrated that administration of pineal polypeptides to the rabbit lowers the urinary 17-KS both of testicular and adrenal origin. Conversely, pinealectomy caused an increase in the level of these steroid hormones. The same pineal material induced a marked decrease both in the serum and testicular testosterone and in the plasma and adrenal corticosterone in the rat. At the same time administration of pineal polypeptides lowered the cholesterol in the serum, bile, liver, testis and adrenal. Melatonin had no effect on these biochemical indices, which demonstrates that the effects obtained with pineal polypeptides are not mediated by melatonin.", "contents": "The antisteroid action on the pineal gland. The study presents the data gathered along the years on the antisteroid action of the pineal gland. Thus, it was demonstrated that administration of pineal polypeptides to the rabbit lowers the urinary 17-KS both of testicular and adrenal origin. Conversely, pinealectomy caused an increase in the level of these steroid hormones. The same pineal material induced a marked decrease both in the serum and testicular testosterone and in the plasma and adrenal corticosterone in the rat. At the same time administration of pineal polypeptides lowered the cholesterol in the serum, bile, liver, testis and adrenal. Melatonin had no effect on these biochemical indices, which demonstrates that the effects obtained with pineal polypeptides are not mediated by melatonin.", "PMID": 973107}
{"id": "pubmed23n0032_9160", "title": "Endocrine implications in experimental tumoral metastasis. III. The influence of the thyroid status on the metastasis of 256 Walker carcinosarcoma intratesticularly grafted and on the in vitro proliferation of the tumoral cells.", "content": "The influence of thyroid status modifications on the metastasis of 256 Walker carcinosarcoma intratesticularly grafted to rats was examined. Inhibition of metastasization and of the secondary tumoral growth was noticed in animals chronically treated with thyroxine, while hypothyroidization realized either by surgery or by administration of synthetical antithyroid drugs enhanced metastasization and growth of the secondary tumors. The primary tumoral development was not significantly influenced by the thyroid status modifications. The variations of the thyroid hormonal level influenced nonspecifically the proliferation of the in vitro cultivated tumoral cells.", "contents": "Endocrine implications in experimental tumoral metastasis. III. The influence of the thyroid status on the metastasis of 256 Walker carcinosarcoma intratesticularly grafted and on the in vitro proliferation of the tumoral cells. The influence of thyroid status modifications on the metastasis of 256 Walker carcinosarcoma intratesticularly grafted to rats was examined. Inhibition of metastasization and of the secondary tumoral growth was noticed in animals chronically treated with thyroxine, while hypothyroidization realized either by surgery or by administration of synthetical antithyroid drugs enhanced metastasization and growth of the secondary tumors. The primary tumoral development was not significantly influenced by the thyroid status modifications. The variations of the thyroid hormonal level influenced nonspecifically the proliferation of the in vitro cultivated tumoral cells.", "PMID": 973109}
{"id": "pubmed23n0032_9161", "title": "Air ions and the urinary 17-ketosteroids.", "content": "The authors correlated the aeroions variations in January, February and March 1963 with urinary 17-KS variations in 5 adult subjects. The results revealed that the excess of positive aeroions induced an increase of the urinary 17-KS eliminations, whereas the excess of negative aeroions induced a decrease.", "contents": "Air ions and the urinary 17-ketosteroids. The authors correlated the aeroions variations in January, February and March 1963 with urinary 17-KS variations in 5 adult subjects. The results revealed that the excess of positive aeroions induced an increase of the urinary 17-KS eliminations, whereas the excess of negative aeroions induced a decrease.", "PMID": 973110}
{"id": "pubmed23n0032_9162", "title": "Influence of the thymus polypeptide (TP) extracts on the mouse inoculated with influenza virus.", "content": "Administration of thymus polypeptide (TP) extracts to the mouse inoculated with the A2 AICHI influenza virus strain, causes a significant decrease in hemagglutination titres in the lung cells suspensions of the treated animals as compared to the nontreated controls.", "contents": "Influence of the thymus polypeptide (TP) extracts on the mouse inoculated with influenza virus. Administration of thymus polypeptide (TP) extracts to the mouse inoculated with the A2 AICHI influenza virus strain, causes a significant decrease in hemagglutination titres in the lung cells suspensions of the treated animals as compared to the nontreated controls.", "PMID": 973108}
{"id": "pubmed23n0032_9163", "title": "Skeletal calcium retention in hyperthyroidism and in vitro calcitonin inactivation.", "content": "Studies on patients with severe hyperthyroidism revealed an increased skeletal avidity for calcium, expressed in terms of net retention, after a three-hour i.v. load of 10mg Ca/kg body weight. For the same hyperthyroid subjects the calcitonin inactivating potency of the sera was tested in vitro. The results were expressed as restant hypocalcemic potency of the calcitonin after incubation with the serum fractions. In hyperthyroid subjects two fractions in dilutions of 1:10 reduced the hypocalcemic activity of calcitonin by 75%. In normals this activity appeared only in one fraction. All these results suggest a prompt calcitonin release during calcium infusion which is however less efficient, probably because of the increased amount of calcitonin inactivating factor present in sera. The serum inactivating factor generally contributes to the short-time activity of calcitonin both in normals and in some clinical states with bone calcium imbalance.", "contents": "Skeletal calcium retention in hyperthyroidism and in vitro calcitonin inactivation. Studies on patients with severe hyperthyroidism revealed an increased skeletal avidity for calcium, expressed in terms of net retention, after a three-hour i.v. load of 10mg Ca/kg body weight. For the same hyperthyroid subjects the calcitonin inactivating potency of the sera was tested in vitro. The results were expressed as restant hypocalcemic potency of the calcitonin after incubation with the serum fractions. In hyperthyroid subjects two fractions in dilutions of 1:10 reduced the hypocalcemic activity of calcitonin by 75%. In normals this activity appeared only in one fraction. All these results suggest a prompt calcitonin release during calcium infusion which is however less efficient, probably because of the increased amount of calcitonin inactivating factor present in sera. The serum inactivating factor generally contributes to the short-time activity of calcitonin both in normals and in some clinical states with bone calcium imbalance.", "PMID": 973111}
{"id": "pubmed23n0032_9164", "title": "Child with 46, XY/46, XY, 18p- mosaic.", "content": "A 46, XY/46, XY 18 p-mosaic was encountered in a child with cranio-facial dysmorphia, anomalies of the fingers, metaphyseal and epiphyseal dysgenesis, psycho-motor backwardness, anomalies of EEG and convulsions.", "contents": "Child with 46, XY/46, XY, 18p- mosaic. A 46, XY/46, XY 18 p-mosaic was encountered in a child with cranio-facial dysmorphia, anomalies of the fingers, metaphyseal and epiphyseal dysgenesis, psycho-motor backwardness, anomalies of EEG and convulsions.", "PMID": 973114}
{"id": "pubmed23n0032_9165", "title": "Gonadotropin secretion in patients with gonadal dysgenesis.", "content": "Plasma FSH and LH levels were investigated by the radioimmunological method of Burr et al. [1] in 35 patients with gonadal dysgenesis. In addition ten healthy, normally menstruating women were examined as controls. Plasma FSH levels were significantly elevated in the patients with gonadal dysgenesis, while LH concentration disclosed no characteristic alteration. There was no specific correlation between the plasma FSH and LH levels. The data obtained add further support to the idea that gonadotropin synthesis and release may be differentially affected by the gonadal steroids.", "contents": "Gonadotropin secretion in patients with gonadal dysgenesis. Plasma FSH and LH levels were investigated by the radioimmunological method of Burr et al. [1] in 35 patients with gonadal dysgenesis. In addition ten healthy, normally menstruating women were examined as controls. Plasma FSH levels were significantly elevated in the patients with gonadal dysgenesis, while LH concentration disclosed no characteristic alteration. There was no specific correlation between the plasma FSH and LH levels. The data obtained add further support to the idea that gonadotropin synthesis and release may be differentially affected by the gonadal steroids.", "PMID": 973112}
{"id": "pubmed23n0032_9166", "title": "Disturbances in psychological functions of workers occupationally exposed to styrene.", "content": "The relationship between long-term occupational styrene exposure and disturbances in psychological functions was studied. The exposed group consisted of 98 male workers exposed to styrene in the manufacture of polyester plastic products, and the comparison group comprised 43 concrete reinforcement workers. The duration of exposure in years and the mean mandelic acid concentration in urine (five determinations during 5 weeks before the clinical examination) and the cumulative dose of exposure were used as the indicators of exposure. The psychological functions studied were intelligence, visuomotor speed, visuomotor accuracy, memory, vigilance, and psychomotor performance. Some personality test variables were also included. In a group comparison two variables (visuomotor inaccuracy and poor psychomotor performance) out of 20 showed statistically significant differences and characterized the styrene exposed group. These findings were confirmed when workers with low and high styrene exposure were compared. One variable measuring visuomotor speed and another measuring visual memory were related to the duration of exposure (R = 0.28, p less than 0.05). In a multiple regression analysis disturbances in visuomotor accuracy, poor psychomotor performance, and lowered vigilance proved to have some connection with a high mandelic acid concentration (R = 0.49, p less than 0.01). In addition workers with disturbances in visuomotor accuracy and slight disturbances in virumotor speed had higher mandelic acid concentrations when compared with those workers without any deterioration. The results indicate that disturbances in visuomotor accuracy and, to a lesser degree, in psychomotor performance are the main findings measured by test methods used in this study and related to one indicator of styrene exposure, mandelic acid concentration. The psychological methods used revealed subclinical symptoms related to exposure, and they should aid in making group diagnoses, e.g., when additional information is needed for the determination of the threshold limit value. The deterioration found in visuomotor accuracy could be related to lowered safety in work and traffic.", "contents": "Disturbances in psychological functions of workers occupationally exposed to styrene. The relationship between long-term occupational styrene exposure and disturbances in psychological functions was studied. The exposed group consisted of 98 male workers exposed to styrene in the manufacture of polyester plastic products, and the comparison group comprised 43 concrete reinforcement workers. The duration of exposure in years and the mean mandelic acid concentration in urine (five determinations during 5 weeks before the clinical examination) and the cumulative dose of exposure were used as the indicators of exposure. The psychological functions studied were intelligence, visuomotor speed, visuomotor accuracy, memory, vigilance, and psychomotor performance. Some personality test variables were also included. In a group comparison two variables (visuomotor inaccuracy and poor psychomotor performance) out of 20 showed statistically significant differences and characterized the styrene exposed group. These findings were confirmed when workers with low and high styrene exposure were compared. One variable measuring visuomotor speed and another measuring visual memory were related to the duration of exposure (R = 0.28, p less than 0.05). In a multiple regression analysis disturbances in visuomotor accuracy, poor psychomotor performance, and lowered vigilance proved to have some connection with a high mandelic acid concentration (R = 0.49, p less than 0.01). In addition workers with disturbances in visuomotor accuracy and slight disturbances in virumotor speed had higher mandelic acid concentrations when compared with those workers without any deterioration. The results indicate that disturbances in visuomotor accuracy and, to a lesser degree, in psychomotor performance are the main findings measured by test methods used in this study and related to one indicator of styrene exposure, mandelic acid concentration. The psychological methods used revealed subclinical symptoms related to exposure, and they should aid in making group diagnoses, e.g., when additional information is needed for the determination of the threshold limit value. The deterioration found in visuomotor accuracy could be related to lowered safety in work and traffic.", "PMID": 973126}
{"id": "pubmed23n0032_9167", "title": "Neurophysiological findings among workers occupationally exposed to styrene.", "content": "Ninety-six male workers occupationally exposured to styrene in 24 plants producing reinforced polyester plastic products were studied neurophysiologically. The mean age of the subjects was 29.6 +/- 7.4 years, and the duration of the exposure varied from 6 months to 14 years (mean 5.0 years). Mandelic acid concentration in the urine (mean of five values determined during five consecutive weeks) was used as the measure of exposure. The individual means of the mandelic acid concentrations varied from 7 to 4,715 mg/dm3 (median 808 mg/dm3). Neurotoxic effects of styrene exposure upon the central nervous system were revealed by abnormalities in the EEG. The overall prevalence of abnormal EEGs was 24% (23 out of 96), which is higher than that found among a normal population (p less than 0.05). Abnormal EEGs were found in one-third of the subjects with relevant styrene exposure (mandelic acid concentration over 700 mg/dm3), while low-level styrene exposure did not increase the prevalence of normal EEGs above that found in a normal population. Nerve conduction velocity measurements performed on 40 subjects did not reveal any definite relationship between neuropathy and styrene exposure. The EEG examination is useful in the investigation of the neurotoxic effects of styrene. Whenever a possibility exists of excessive styrene exposure or beginning poisoning, the workers should be submitted to an EEG examination.", "contents": "Neurophysiological findings among workers occupationally exposed to styrene. Ninety-six male workers occupationally exposured to styrene in 24 plants producing reinforced polyester plastic products were studied neurophysiologically. The mean age of the subjects was 29.6 +/- 7.4 years, and the duration of the exposure varied from 6 months to 14 years (mean 5.0 years). Mandelic acid concentration in the urine (mean of five values determined during five consecutive weeks) was used as the measure of exposure. The individual means of the mandelic acid concentrations varied from 7 to 4,715 mg/dm3 (median 808 mg/dm3). Neurotoxic effects of styrene exposure upon the central nervous system were revealed by abnormalities in the EEG. The overall prevalence of abnormal EEGs was 24% (23 out of 96), which is higher than that found among a normal population (p less than 0.05). Abnormal EEGs were found in one-third of the subjects with relevant styrene exposure (mandelic acid concentration over 700 mg/dm3), while low-level styrene exposure did not increase the prevalence of normal EEGs above that found in a normal population. Nerve conduction velocity measurements performed on 40 subjects did not reveal any definite relationship between neuropathy and styrene exposure. The EEG examination is useful in the investigation of the neurotoxic effects of styrene. Whenever a possibility exists of excessive styrene exposure or beginning poisoning, the workers should be submitted to an EEG examination.", "PMID": 973127}
{"id": "pubmed23n0032_9168", "title": "Long-term exposure to jet fuel: an investigation on occupationally exposed workers with special reference to the nervous system.", "content": "In the present study the results of a neurological and neurophysiological health examination of 29 aircraft factory workers chronically exposed to jet fuel vapors are presented. The exposed subjects were classified into a heavily exposed and a less heavily exposed group. The examination included a standardized clinical neurological examination, measurements of the conduction velocities in the peripheral nerves, and threshold determinations of vibratory sensations in the extremities. All 13 persons examined in the heavily exposed group and 7 of the 16 in the less heavily exposed group stated that they had repeatedly experienced acute effects (dizziness, respiratory tract symptoms, heart palpitations, a feeling of pressure on the chest, nausea, headache) of the jet fuel vapors in the inhaled air. A high rate of symptoms indicative of neurasthenia and psychasthenia and symptoms and signs indicative of polyneuropathy was observed both in the heavily exposed group and in the two groups combined in comparison with reference groups. Considering the presented facts concerning (a) the acute effects on repeated occasions, (b) the high rates of symptoms indicative of neurasthenia and psychasthenia and symptoms and signs indicative of polyneuropathy, and (c) the differences in the observations made between the two groups with varying degrees of exposure to jet fuel, the authors interpreted the results as indicative of a possible effect of long-term exposure to jet fuel on the nervous system.", "contents": "Long-term exposure to jet fuel: an investigation on occupationally exposed workers with special reference to the nervous system. In the present study the results of a neurological and neurophysiological health examination of 29 aircraft factory workers chronically exposed to jet fuel vapors are presented. The exposed subjects were classified into a heavily exposed and a less heavily exposed group. The examination included a standardized clinical neurological examination, measurements of the conduction velocities in the peripheral nerves, and threshold determinations of vibratory sensations in the extremities. All 13 persons examined in the heavily exposed group and 7 of the 16 in the less heavily exposed group stated that they had repeatedly experienced acute effects (dizziness, respiratory tract symptoms, heart palpitations, a feeling of pressure on the chest, nausea, headache) of the jet fuel vapors in the inhaled air. A high rate of symptoms indicative of neurasthenia and psychasthenia and symptoms and signs indicative of polyneuropathy was observed both in the heavily exposed group and in the two groups combined in comparison with reference groups. Considering the presented facts concerning (a) the acute effects on repeated occasions, (b) the high rates of symptoms indicative of neurasthenia and psychasthenia and symptoms and signs indicative of polyneuropathy, and (c) the differences in the observations made between the two groups with varying degrees of exposure to jet fuel, the authors interpreted the results as indicative of a possible effect of long-term exposure to jet fuel on the nervous system.", "PMID": 973128}
{"id": "pubmed23n0032_9169", "title": "Exposure to butyl alcohol: uptake and distribution in man.", "content": "Twelve subjects were exposed to 300 or 600 mg/m3 of butyl alcohol in inspired air during rest and during exercise on a bicycle ergometer. Exposure lasted 2 h. The results were puzzling in view of the high blood/air partition coefficient for butyl alcohol. The arterial blood concentration was low. The concentration in the last part of the expired air, i.e., the \"\"alveolar'' concentration, was low. The quotient of \"\"alveolar'' concentration X 100/inspired concentration was low in relation to the low percentage uptake. However the high solubility of butyl alcohol in water may explain the results. Butyl alcohol was probably partially taken up in the water of the dead space mucous membranes during inspiration. It was then partially released from the membranes. Therefore the concentration of butyl alcohol in the last part of expiration was probably not the same as the concentration in the alveolar air.", "contents": "Exposure to butyl alcohol: uptake and distribution in man. Twelve subjects were exposed to 300 or 600 mg/m3 of butyl alcohol in inspired air during rest and during exercise on a bicycle ergometer. Exposure lasted 2 h. The results were puzzling in view of the high blood/air partition coefficient for butyl alcohol. The arterial blood concentration was low. The concentration in the last part of the expired air, i.e., the \"\"alveolar'' concentration, was low. The quotient of \"\"alveolar'' concentration X 100/inspired concentration was low in relation to the low percentage uptake. However the high solubility of butyl alcohol in water may explain the results. Butyl alcohol was probably partially taken up in the water of the dead space mucous membranes during inspiration. It was then partially released from the membranes. Therefore the concentration of butyl alcohol in the last part of expiration was probably not the same as the concentration in the alveolar air.", "PMID": 973129}
{"id": "pubmed23n0032_9170", "title": "A method for determining benzo(a)pyrene in air samples collected on glass fiber filters in occupational areas.", "content": "Benzo(a)pyrene was analyzed in air samples collected on glass fiber filters in occupational areas. Vacuum sublimation, thin-layer chromatography on acetylated cellulose, and fluorescence scanning of the thin layer plate were performed. The recovery of the method was estimated by an isotope dilution technique using 3H-benzo(a)pyrene. Special equipment was constructed for sublimation. The detection limit for authentic samples was found to be about 10 pmoles per filter.", "contents": "A method for determining benzo(a)pyrene in air samples collected on glass fiber filters in occupational areas. Benzo(a)pyrene was analyzed in air samples collected on glass fiber filters in occupational areas. Vacuum sublimation, thin-layer chromatography on acetylated cellulose, and fluorescence scanning of the thin layer plate were performed. The recovery of the method was estimated by an isotope dilution technique using 3H-benzo(a)pyrene. Special equipment was constructed for sublimation. The detection limit for authentic samples was found to be about 10 pmoles per filter.", "PMID": 973130}
{"id": "pubmed23n0032_9171", "title": "Determination of exposure to electric fields in extra high voltage substations.", "content": "Electrophysical effects related to extra high voltage are surveyed for the determination of the exposure of personnel to electric fields in substations. It is concluded that the electric field strengths and the electrical discharges to the personnel are the important electrophysical factors. Instruments for measuring the field strength at grounded surfaces and at nonzero potentials were constructed. Results are presented of measurements with these instruments in substations. A dummy was used for the measurement of the distribution of capacitive currents to a man. The dummy can also be used for measuring the effectiveness of special shielding clothes.", "contents": "Determination of exposure to electric fields in extra high voltage substations. Electrophysical effects related to extra high voltage are surveyed for the determination of the exposure of personnel to electric fields in substations. It is concluded that the electric field strengths and the electrical discharges to the personnel are the important electrophysical factors. Instruments for measuring the field strength at grounded surfaces and at nonzero potentials were constructed. Results are presented of measurements with these instruments in substations. A dummy was used for the measurement of the distribution of capacitive currents to a man. The dummy can also be used for measuring the effectiveness of special shielding clothes.", "PMID": 973131}
{"id": "pubmed23n0032_9172", "title": "Carbon monoxide: effects on oxygenation of the fetus in utero.", "content": "The partial pressure of oxygen in fetal blood decreases in proportion to the carboxyhemoglobin concentrations in fetal and maternal blood. Because fetal oxygen tensions normally equal 20 to 30 percent of the values for adults, this reduction can result in severe hypoxia of vital tissues. Decreases in oxygen tension may be a factor in the lower birth weights of infants born to women who smoke or are exposed to severe air pollution.", "contents": "Carbon monoxide: effects on oxygenation of the fetus in utero. The partial pressure of oxygen in fetal blood decreases in proportion to the carboxyhemoglobin concentrations in fetal and maternal blood. Because fetal oxygen tensions normally equal 20 to 30 percent of the values for adults, this reduction can result in severe hypoxia of vital tissues. Decreases in oxygen tension may be a factor in the lower birth weights of infants born to women who smoke or are exposed to severe air pollution.", "PMID": 973133}
{"id": "pubmed23n0032_9173", "title": "Thyroxine-induced activation of hypothalamo-hypophysial axis in neotenic salamander larvae.", "content": "Thyroxine injected into the hypothalamus of neotenic Ambystoma tigrinum induces metamorphosis by activating hypothalamo-hypophysial stimulatory control of thyroid activity, thereby removing the hypothalamic block to metamorphosis.", "contents": "Thyroxine-induced activation of hypothalamo-hypophysial axis in neotenic salamander larvae. Thyroxine injected into the hypothalamus of neotenic Ambystoma tigrinum induces metamorphosis by activating hypothalamo-hypophysial stimulatory control of thyroid activity, thereby removing the hypothalamic block to metamorphosis.", "PMID": 973134}
{"id": "pubmed23n0032_9174", "title": "Ribosomal genes of Xenopus laevis: evidence of nucleosomes in transcriptionally active chromatin.", "content": "Most of the reiterated ribosomal genes in the somatic cells of larvae of a mutant of Xenopus laevis appear to be protected from short-term nuclease digestion by being packaged in the form of chromatin subunits or nucleosomes. Since these mutant animals probably require all of their ribosomal genes to be active in order to maintain viability, at least some of the transcriptionally active gene sequences are probably associated with chromatin subunits. Thus, association of DNA with nucleosomes may not necessarily preclude template activity, although such association is probably of a dynamic rather than a static nature.", "contents": "Ribosomal genes of Xenopus laevis: evidence of nucleosomes in transcriptionally active chromatin. Most of the reiterated ribosomal genes in the somatic cells of larvae of a mutant of Xenopus laevis appear to be protected from short-term nuclease digestion by being packaged in the form of chromatin subunits or nucleosomes. Since these mutant animals probably require all of their ribosomal genes to be active in order to maintain viability, at least some of the transcriptionally active gene sequences are probably associated with chromatin subunits. Thus, association of DNA with nucleosomes may not necessarily preclude template activity, although such association is probably of a dynamic rather than a static nature.", "PMID": 973136}
{"id": "pubmed23n0032_9175", "title": "Derepressed alloantigen in transplacentally induced lung tumor coded for by H-2 linked gene.", "content": "A transplacentally induced lung tumor of strain C3Hf mice grows progressively when transplanted to (C3Hf X A)F1 hybrid mice but not when transplanted to C3Hf recipients. Progressive tumor growth occurs in [(C3Hf X A)F1 X C3Hf] backcross mice inheriting the H-2a haplotype from the F1 parent. Furthermore, radioresistant immunity to the lung tumor can be induced in C3Hf mice by immunization with normal tissue of B10.A and B10.A(2R) but not of B10 or B10.A(5R) strain mice.", "contents": "Derepressed alloantigen in transplacentally induced lung tumor coded for by H-2 linked gene. A transplacentally induced lung tumor of strain C3Hf mice grows progressively when transplanted to (C3Hf X A)F1 hybrid mice but not when transplanted to C3Hf recipients. Progressive tumor growth occurs in [(C3Hf X A)F1 X C3Hf] backcross mice inheriting the H-2a haplotype from the F1 parent. Furthermore, radioresistant immunity to the lung tumor can be induced in C3Hf mice by immunization with normal tissue of B10.A and B10.A(2R) but not of B10 or B10.A(5R) strain mice.", "PMID": 973137}
{"id": "pubmed23n0032_9176", "title": "Central nervous regulation of body temperature during sleep.", "content": "The relationship between hypothalamic temperature and metabolic heat production was measured during wakefulness, slow-wave sleep, and paradoxical sleep in unrestrained kangaroo rats (Dipodomys). Hypothalamic temperature was manipulated with chronically implanted, water-perfused thermodes while cortical electroencephalogram, electromyogram, metabolic rate, and body movement were continuously recorded. During slow-wave sleep, in comparison to wakefulness, there is a lowered threshold hypothalamic temperature for the metabolic heat production response and a lowered proportionality constant relating rate of metabolic heat production to hypothalamic temperature. During paradoxical sleep no increase in metabolic heat production could be elicited by lowering hypothalamic temperature, which indicates that the thermoregulatory system is inoperative. These results provide a basis for explaining the changes in various body temperatures, metabolic rate, and other thermoregulatory responses during sleep in a variety of mammals.", "contents": "Central nervous regulation of body temperature during sleep. The relationship between hypothalamic temperature and metabolic heat production was measured during wakefulness, slow-wave sleep, and paradoxical sleep in unrestrained kangaroo rats (Dipodomys). Hypothalamic temperature was manipulated with chronically implanted, water-perfused thermodes while cortical electroencephalogram, electromyogram, metabolic rate, and body movement were continuously recorded. During slow-wave sleep, in comparison to wakefulness, there is a lowered threshold hypothalamic temperature for the metabolic heat production response and a lowered proportionality constant relating rate of metabolic heat production to hypothalamic temperature. During paradoxical sleep no increase in metabolic heat production could be elicited by lowering hypothalamic temperature, which indicates that the thermoregulatory system is inoperative. These results provide a basis for explaining the changes in various body temperatures, metabolic rate, and other thermoregulatory responses during sleep in a variety of mammals.", "PMID": 973138}
{"id": "pubmed23n0032_9177", "title": "Dopaminergic agents: influence on serotonin in the molluscan nervous system.", "content": "Treatment of the mussel Mytilus edulis with 6-hydroxydopamine or with alpha-methyl-p-tyrosine decreased dopamine and increased serotonin in the nervous system. Treatment with dopamine decreased serotonin concentrations and prevented the effect of 6-hydroxydopamine. The serotonin concentration appears to be determined in part by the concentration of dopamine.", "contents": "Dopaminergic agents: influence on serotonin in the molluscan nervous system. Treatment of the mussel Mytilus edulis with 6-hydroxydopamine or with alpha-methyl-p-tyrosine decreased dopamine and increased serotonin in the nervous system. Treatment with dopamine decreased serotonin concentrations and prevented the effect of 6-hydroxydopamine. The serotonin concentration appears to be determined in part by the concentration of dopamine.", "PMID": 973139}
{"id": "pubmed23n0032_9178", "title": "Disproportionate tonotopic representation for processing CF-FM sonar signals in the mustache bat auditory cortex.", "content": "The extent of cortical representation of the peripheral sensory field depends on its importance for species behavior. The orientation sound of the mustache bat (Pteronotus parnellii rubiginosus) invariably consists of long constant-frequency and short frequency-modulated components and is indispensable for its survival. A disproportionately large part of the auditory cortex of this bat is occupied by neurons processing the predominant components in the orientation signal and Doppler-shifted echoes. This disproportionate cortical representation related to features of biologically significant signals is comparable to that in the somatosensory and visual systems in many mammals, but it has not previously been observed in the auditory system.", "contents": "Disproportionate tonotopic representation for processing CF-FM sonar signals in the mustache bat auditory cortex. The extent of cortical representation of the peripheral sensory field depends on its importance for species behavior. The orientation sound of the mustache bat (Pteronotus parnellii rubiginosus) invariably consists of long constant-frequency and short frequency-modulated components and is indispensable for its survival. A disproportionately large part of the auditory cortex of this bat is occupied by neurons processing the predominant components in the orientation signal and Doppler-shifted echoes. This disproportionate cortical representation related to features of biologically significant signals is comparable to that in the somatosensory and visual systems in many mammals, but it has not previously been observed in the auditory system.", "PMID": 973140}
{"id": "pubmed23n0032_9179", "title": "[Organization of reanimation and intensive therapy in pediatrics].", "content": "The authors propose a definitely-arranged system for organization of reanimation and intensive therapy of children. It includes 5 component elements, viz. rooms and posts of reanimation aid at kindergartens and creches, polyclinics and sanatoria; intensive therapy wards at maternity homes and pediatric hospitals; anesthesiological, reanimation and intensive therapy departments of multispecialized pediatric inpatient departments; reanimation and anesthesiological centers; a consultative travelling reanimation pediatric team. The structural form of the patients' hospitalization at pediatric reanimation departments is analyzed. Of their total number 48 per cent are surgical patients after serious operative interventions on the organs of thoracic and abdominal cavities with multiple injuries and malformations; 27.1 per cent are infectious patients, 11.9 per cent - those suffering from poisonings and 10.7 per cent patients with somatic illnesses.", "contents": "[Organization of reanimation and intensive therapy in pediatrics]. The authors propose a definitely-arranged system for organization of reanimation and intensive therapy of children. It includes 5 component elements, viz. rooms and posts of reanimation aid at kindergartens and creches, polyclinics and sanatoria; intensive therapy wards at maternity homes and pediatric hospitals; anesthesiological, reanimation and intensive therapy departments of multispecialized pediatric inpatient departments; reanimation and anesthesiological centers; a consultative travelling reanimation pediatric team. The structural form of the patients' hospitalization at pediatric reanimation departments is analyzed. Of their total number 48 per cent are surgical patients after serious operative interventions on the organs of thoracic and abdominal cavities with multiple injuries and malformations; 27.1 per cent are infectious patients, 11.9 per cent - those suffering from poisonings and 10.7 per cent patients with somatic illnesses.", "PMID": 973146}
{"id": "pubmed23n0032_9180", "title": "[Morbidity with temproary work loss among miners and engineering-technical personnel in the iron ore industry].", "content": "The influence of industrial-occupational and social-hygienic factors (vocation, age, underground and continuous service record, per capita income, nutrition) on the level of temporary incapacitation and also on the dynamics and structure of the disease incidence among average annually engaged and regular miners in ore-mining industry was studied. The use of multifactor correlation and regression analysis and of the Bayes probability method helped establish the dependence of the temporary incapacitation level on the age, occupational activities, continuous service record, family composition, education and per capita income. The administration and social bodies of the mine in concerd with medical workers have on the ground of the present investigation outlined ways and means for implementation of measures aimed at consolidating the health status of workers.", "contents": "[Morbidity with temproary work loss among miners and engineering-technical personnel in the iron ore industry]. The influence of industrial-occupational and social-hygienic factors (vocation, age, underground and continuous service record, per capita income, nutrition) on the level of temporary incapacitation and also on the dynamics and structure of the disease incidence among average annually engaged and regular miners in ore-mining industry was studied. The use of multifactor correlation and regression analysis and of the Bayes probability method helped establish the dependence of the temporary incapacitation level on the age, occupational activities, continuous service record, family composition, education and per capita income. The administration and social bodies of the mine in concerd with medical workers have on the ground of the present investigation outlined ways and means for implementation of measures aimed at consolidating the health status of workers.", "PMID": 973147}
{"id": "pubmed23n0032_9181", "title": "[Registration files on the dispensary coverage of the population].", "content": "To-day the quality of treatment on the dispensary levels is the most important factor in the work of medico-prophylactic establishments. The existing registration files on the dispensary coverage of thee population needs revision with a view of satisfying requirements of practicians. The organizational-methodological division of th Lvov regional clinical hospital has devised an optimal variant of registration forms that enable it to ensure keeping more exact records of patients undergoing the dispendary treatment, to cut down the time spend in checking, to more quickly obtain the needed current data and to strike balance of the medical commissions work in carrying out periodical medical examinations of the population.", "contents": "[Registration files on the dispensary coverage of the population]. To-day the quality of treatment on the dispensary levels is the most important factor in the work of medico-prophylactic establishments. The existing registration files on the dispensary coverage of thee population needs revision with a view of satisfying requirements of practicians. The organizational-methodological division of th Lvov regional clinical hospital has devised an optimal variant of registration forms that enable it to ensure keeping more exact records of patients undergoing the dispendary treatment, to cut down the time spend in checking, to more quickly obtain the needed current data and to strike balance of the medical commissions work in carrying out periodical medical examinations of the population.", "PMID": 973149}
{"id": "pubmed23n0032_9182", "title": "Clinical and EEG response to clonazepam in four patients with self-induced photosensitive epilepsy.", "content": "Clonazepam was administered to 4 patients with an unusual form of photosensitive epilepsy. Two showed immediate and sustained clinical and electro-encephalographic (EEG) improvement. One showed immediate but unsustained clinical improvement, but there was no EEG improvement. One patient did not improve either clinically or on EEG.", "contents": "Clinical and EEG response to clonazepam in four patients with self-induced photosensitive epilepsy. Clonazepam was administered to 4 patients with an unusual form of photosensitive epilepsy. Two showed immediate and sustained clinical and electro-encephalographic (EEG) improvement. One showed immediate but unsustained clinical improvement, but there was no EEG improvement. One patient did not improve either clinically or on EEG.", "PMID": 973159}
{"id": "pubmed23n0032_9183", "title": "Anaerobic infections in hospital practice.", "content": "The role of anaerobes in the pathogenesis of infections was investigated. Anaerobes were isolated from 0,25% of blood cultures and from 15,8% of other specimens tested; in 15,1% of cases where anaerobes were isolated, no aerobes were found. The strains most commonly encountered were Bacteroides fragilis, B. melaninogenicus, Peptostreptococcus anaerobius and Clostridium perfringens. Sensitivity tests in vitro showed all organisms to be sensitive to clindamycin, metronidazole and chloramphenicol, while a large proportion were resistant to penicillin G and smaller numbers were resistant to tetracycline.", "contents": "Anaerobic infections in hospital practice. The role of anaerobes in the pathogenesis of infections was investigated. Anaerobes were isolated from 0,25% of blood cultures and from 15,8% of other specimens tested; in 15,1% of cases where anaerobes were isolated, no aerobes were found. The strains most commonly encountered were Bacteroides fragilis, B. melaninogenicus, Peptostreptococcus anaerobius and Clostridium perfringens. Sensitivity tests in vitro showed all organisms to be sensitive to clindamycin, metronidazole and chloramphenicol, while a large proportion were resistant to penicillin G and smaller numbers were resistant to tetracycline.", "PMID": 973160}
{"id": "pubmed23n0032_9184", "title": "Ankylosing spondylitis in a Xhosa father and daughter.", "content": "A Xhosa father and daughter with ankylosing spondylitis are reported. Review of the literature revealed a low incidence of ankylosing spondylitis in Blacks in general, with only isolated reports from the continent of Africa itself. To our knowledge, there has been no documentation of ankylosing spondylitis in a black female, or of ankylosing spondylitis in first-degree Black relatives. The clinical features of ankylosing spondylitis in the female, and the role of human leucocyte antigen (HLA) B27 in the disease are also discussed.", "contents": "Ankylosing spondylitis in a Xhosa father and daughter. A Xhosa father and daughter with ankylosing spondylitis are reported. Review of the literature revealed a low incidence of ankylosing spondylitis in Blacks in general, with only isolated reports from the continent of Africa itself. To our knowledge, there has been no documentation of ankylosing spondylitis in a black female, or of ankylosing spondylitis in first-degree Black relatives. The clinical features of ankylosing spondylitis in the female, and the role of human leucocyte antigen (HLA) B27 in the disease are also discussed.", "PMID": 973161}
{"id": "pubmed23n0032_9185", "title": "Hypoglycaemia associated with severe kwashiorkor.", "content": "A study was designed to investigate the hypoglycaemia of severe kwashiorkor. The findings of significant stores of hepatic glycogen suggests that glycogenolysis was impaired. Plasma glucagon levels were relatively low, and it is suggested that this might be secondary to either a pancreatic alpha-cell defect, or inadequate sympathetic stimulation.", "contents": "Hypoglycaemia associated with severe kwashiorkor. A study was designed to investigate the hypoglycaemia of severe kwashiorkor. The findings of significant stores of hepatic glycogen suggests that glycogenolysis was impaired. Plasma glucagon levels were relatively low, and it is suggested that this might be secondary to either a pancreatic alpha-cell defect, or inadequate sympathetic stimulation.", "PMID": 973162}
{"id": "pubmed23n0032_9186", "title": "Vitiligo and malignant melanoma: a significant association?", "content": "Three patients with malignant melanoma and coexisting vitiligo are described. All three appear to exhibit at least partial spontaneous control of the melanoma. The hypothesis is made that there may be a significant association between vitiligo and regressing melanoma. The basis for this is the fact that there may be an association between vitiligo and the production of an antimelanoma antibody, promoting rejection of the melanoma.", "contents": "Vitiligo and malignant melanoma: a significant association? Three patients with malignant melanoma and coexisting vitiligo are described. All three appear to exhibit at least partial spontaneous control of the melanoma. The hypothesis is made that there may be a significant association between vitiligo and regressing melanoma. The basis for this is the fact that there may be an association between vitiligo and the production of an antimelanoma antibody, promoting rejection of the melanoma.", "PMID": 973163}
{"id": "pubmed23n0032_9187", "title": "Reye's syndrome.", "content": "The occurrence of Reye's syndrome in 3 children in Bulawayo is reported, and a short review of the diagnostic features and pathogenesis is offered.", "contents": "Reye's syndrome. The occurrence of Reye's syndrome in 3 children in Bulawayo is reported, and a short review of the diagnostic features and pathogenesis is offered.", "PMID": 973164}
{"id": "pubmed23n0032_9188", "title": "Serum immunoglobulins in Black South African children.", "content": "Serum immunoglobulin levels in Black children were estimated and the results analysed in 13 age ranges, from birth up to 15 years of age. Samples from children whose serum immunoglobulins were unlikely to be affected by their clinical condition were chosen for the analysis. The values measured were much higher when compared with conventionally accepted reference ranges in White children within the same age groups. Therefore, it is most important to interpret the results of immunoglobulin measurement within the range for the individual concerned.", "contents": "Serum immunoglobulins in Black South African children. Serum immunoglobulin levels in Black children were estimated and the results analysed in 13 age ranges, from birth up to 15 years of age. Samples from children whose serum immunoglobulins were unlikely to be affected by their clinical condition were chosen for the analysis. The values measured were much higher when compared with conventionally accepted reference ranges in White children within the same age groups. Therefore, it is most important to interpret the results of immunoglobulin measurement within the range for the individual concerned.", "PMID": 973166}
{"id": "pubmed23n0032_9189", "title": "The Cornelia de Lange syndrome: a study of 9 affected individuals.", "content": "Nine children with the Cornelia de Lange syndrome have been examined and investigated. Prominent among the clinically important features are retarded physical and mental development, limb malformation and limited life expectancy. Early diagnosis is important for prognostication and management, and is based on the recognition of the combination of the stigmata and typical facial characteristics.", "contents": "The Cornelia de Lange syndrome: a study of 9 affected individuals. Nine children with the Cornelia de Lange syndrome have been examined and investigated. Prominent among the clinically important features are retarded physical and mental development, limb malformation and limited life expectancy. Early diagnosis is important for prognostication and management, and is based on the recognition of the combination of the stigmata and typical facial characteristics.", "PMID": 973167}
{"id": "pubmed23n0032_9190", "title": "A review of deaths in a paediatric burns unit.", "content": "During a 5-year period, 1970 to 1974, a total of 2 118 new patients were admitted to the Burns Unit of the Red Cross War Memorial Children's Hospital in Cape Town. There were 35 deaths. The main complications and causes of death in these 35 patients are critically analysed and standard management methods are reviewed to highlight mistakes in the management of burns.", "contents": "A review of deaths in a paediatric burns unit. During a 5-year period, 1970 to 1974, a total of 2 118 new patients were admitted to the Burns Unit of the Red Cross War Memorial Children's Hospital in Cape Town. There were 35 deaths. The main complications and causes of death in these 35 patients are critically analysed and standard management methods are reviewed to highlight mistakes in the management of burns.", "PMID": 973168}
{"id": "pubmed23n0032_9191", "title": "Turner's syndrome with XO/XXp- karyotype: a case report.", "content": "An unusual case of Turner's syndrome is presented. The case highlights the importance of chromosome analysis in patients presenting with primary amenorrhoea. This patient demonstrates the effects of mosaicism and the importance of the long and short arms of the X chromosome. Deletion of the short arm has only been reported in 2 previous cases of Turner's syndrome. The absence of the short arm is believed to be responsible for the shortness in stature in these patients.", "contents": "Turner's syndrome with XO/XXp- karyotype: a case report. An unusual case of Turner's syndrome is presented. The case highlights the importance of chromosome analysis in patients presenting with primary amenorrhoea. This patient demonstrates the effects of mosaicism and the importance of the long and short arms of the X chromosome. Deletion of the short arm has only been reported in 2 previous cases of Turner's syndrome. The absence of the short arm is believed to be responsible for the shortness in stature in these patients.", "PMID": 973169}
{"id": "pubmed23n0032_9192", "title": "Ruptured popliteal cyst -- an unusual complication of osteo-arthritis: a case report.", "content": "Synovial rupture produces a syndrome resembling acute thrombophlebitis and it is usually seen in patients with rheumatoid arthritis. The interesting feature reported here is the occurrence of popliteal cyst rupture in a patient with no evidence of inflammatory joint disease. It is important to diagnose this condition correctly and to avoid anticoagulant therapy.", "contents": "Ruptured popliteal cyst -- an unusual complication of osteo-arthritis: a case report. Synovial rupture produces a syndrome resembling acute thrombophlebitis and it is usually seen in patients with rheumatoid arthritis. The interesting feature reported here is the occurrence of popliteal cyst rupture in a patient with no evidence of inflammatory joint disease. It is important to diagnose this condition correctly and to avoid anticoagulant therapy.", "PMID": 973170}
{"id": "pubmed23n0032_9193", "title": "[Tuberculosis of the penis (corpora cavernosa)].", "content": "Tuberculosis of the male urethra and corpora cavernosa is discussed and 3 patients with this rare condition are presented. These patients were among 63 patients who presented with urethral stricture during the past 2 years. The diagnosis was based primarily on histological appearance, and treatment consisted of a combined medical and surgical approach, viz. streptomycin, PAS, INH with or without rifampicin; urinary diversion and drainage of pus (if any); initial stage I urethroplasty after control of infection and stage II urethroplasty after 18 - 20 months.", "contents": "[Tuberculosis of the penis (corpora cavernosa)]. Tuberculosis of the male urethra and corpora cavernosa is discussed and 3 patients with this rare condition are presented. These patients were among 63 patients who presented with urethral stricture during the past 2 years. The diagnosis was based primarily on histological appearance, and treatment consisted of a combined medical and surgical approach, viz. streptomycin, PAS, INH with or without rifampicin; urinary diversion and drainage of pus (if any); initial stage I urethroplasty after control of infection and stage II urethroplasty after 18 - 20 months.", "PMID": 973171}
{"id": "pubmed23n0032_9194", "title": "Physical illness and the psychiatric patient.", "content": "The significance and seriousness of physical illness in mentally ill patients are often not appreciated by those responsible for the immediate care of these patients. This study highlights the nature and prevalence of physical illnesses in a large group of psychiatrically ill patients.", "contents": "Physical illness and the psychiatric patient. The significance and seriousness of physical illness in mentally ill patients are often not appreciated by those responsible for the immediate care of these patients. This study highlights the nature and prevalence of physical illnesses in a large group of psychiatrically ill patients.", "PMID": 973172}
{"id": "pubmed23n0032_9195", "title": "[Inspection of \"christmass rabbits\" (author's transl)].", "content": "403 \"Christmass rabbits\" slaughtered in a public slaughter-house were inspected. The average age of the rabbits was eleven months, the average weight being 4.3 kg. (Rabbits raised for commercial purposes are slaughtered when they are approximately three months and weigh 2.5 kg.). Pathological changes were observed in 34 per cent of the animals after slaughter; these changes were mainly due to parasites. Of all rabbits which were inspected, 77 per cent were passed for food; 13 per cent were only passed after some parts of the animals had been removed (mainly livers showing coccidiosis); 8 per cent were conditionally admitted and 2 per cent were condemned. Moreover, 120 traditionally raised rabbits and 120 rabbits raised for commercial purposes were examined for the presence of Salmonella in the faeces and the wall of the rectum. No Salmonella organisms were detected.", "contents": "[Inspection of \"christmass rabbits\" (author's transl)]. 403 \"Christmass rabbits\" slaughtered in a public slaughter-house were inspected. The average age of the rabbits was eleven months, the average weight being 4.3 kg. (Rabbits raised for commercial purposes are slaughtered when they are approximately three months and weigh 2.5 kg.). Pathological changes were observed in 34 per cent of the animals after slaughter; these changes were mainly due to parasites. Of all rabbits which were inspected, 77 per cent were passed for food; 13 per cent were only passed after some parts of the animals had been removed (mainly livers showing coccidiosis); 8 per cent were conditionally admitted and 2 per cent were condemned. Moreover, 120 traditionally raised rabbits and 120 rabbits raised for commercial purposes were examined for the presence of Salmonella in the faeces and the wall of the rectum. No Salmonella organisms were detected.", "PMID": 973205}
{"id": "pubmed23n0032_9196", "title": "[Preliminary results of a study on the effect of a contagious foot-rot vaccine in sheep (author's transl)].", "content": "A large number of findings on the effects of a contagious foot-rot vaccine have been reported in the literature. To gain a personal understanding of the uses of this vaccine, also in view of foot-rot control as prescribed by law in the Netherlands, the course run by an experimentally induced infection was observed in a flock consisting of vaccinated and non-vaccinated animals. A first impression is that the results may be described as encouraging, in accordance with the findings reported in the literature. The comparative part of the trial having been completed, the studies were continued to see whether vaccination might possibly have a long-term cumulative effect. The motivation and planning of these continued studies will be considered in the discussion of the preliminary results. A report will follow later on.", "contents": "[Preliminary results of a study on the effect of a contagious foot-rot vaccine in sheep (author's transl)]. A large number of findings on the effects of a contagious foot-rot vaccine have been reported in the literature. To gain a personal understanding of the uses of this vaccine, also in view of foot-rot control as prescribed by law in the Netherlands, the course run by an experimentally induced infection was observed in a flock consisting of vaccinated and non-vaccinated animals. A first impression is that the results may be described as encouraging, in accordance with the findings reported in the literature. The comparative part of the trial having been completed, the studies were continued to see whether vaccination might possibly have a long-term cumulative effect. The motivation and planning of these continued studies will be considered in the discussion of the preliminary results. A report will follow later on.", "PMID": 973206}
{"id": "pubmed23n0032_9197", "title": "[Some features of the control of atrophic rhinitis (author's transl)].", "content": "The results obtained by a number of control measures in 152 cases of atrophic rhinitis in the province of North Brabant in 1975 are reviewed. So-called \"elimination of piglets\" from the herd for a few weeks and treatment of the newborn piglets in accordance with a fixed scheme are the measures stressed in this regard moreover, when this scheme of treatment is adopted in a contaminated environment, i.e. without any taking over by the authorities, it also offers certain prospects. The method of control widely used in the Netherlands so far (elimination of piglets not followed by a scheme of treatment) was not sufficiently successful.", "contents": "[Some features of the control of atrophic rhinitis (author's transl)]. The results obtained by a number of control measures in 152 cases of atrophic rhinitis in the province of North Brabant in 1975 are reviewed. So-called \"elimination of piglets\" from the herd for a few weeks and treatment of the newborn piglets in accordance with a fixed scheme are the measures stressed in this regard moreover, when this scheme of treatment is adopted in a contaminated environment, i.e. without any taking over by the authorities, it also offers certain prospects. The method of control widely used in the Netherlands so far (elimination of piglets not followed by a scheme of treatment) was not sufficiently successful.", "PMID": 973207}
{"id": "pubmed23n0032_9198", "title": "The distribution of HLA in a Polynesian population-Western Samoans.", "content": "HLA and gene frequencies are presented for a Polynesian population-the Western Samoans. Within the HLA-A locus A2, A9 and A11 have the highest frequencies and account for 55% of the alleles in this locus. The alleles BW22 and BW40 had the highest frequencies in the HLA-B locus and accounted for 51% of the alleles. The blank gene frequencies for the HLA-A and B loci are .382 and .373 respectively. Significant linkage disequilibrium was found with the haplotypes A1,B7; A3,B7;A2, BW40; A9, BW40 A9, BW22. The most frequent haplotype was A2,BW40. Comparatively low values within this population and between this and other Polynesian populations are discussed in terms of selection, migration and drift.", "contents": "The distribution of HLA in a Polynesian population-Western Samoans. HLA and gene frequencies are presented for a Polynesian population-the Western Samoans. Within the HLA-A locus A2, A9 and A11 have the highest frequencies and account for 55% of the alleles in this locus. The alleles BW22 and BW40 had the highest frequencies in the HLA-B locus and accounted for 51% of the alleles. The blank gene frequencies for the HLA-A and B loci are .382 and .373 respectively. Significant linkage disequilibrium was found with the haplotypes A1,B7; A3,B7;A2, BW40; A9, BW40 A9, BW22. The most frequent haplotype was A2,BW40. Comparatively low values within this population and between this and other Polynesian populations are discussed in terms of selection, migration and drift.", "PMID": 973209}
{"id": "pubmed23n0032_9199", "title": "HLA-SD antigens and schizophrenia: statistical and genetical considerations.", "content": "The HLA-SD phenotype distributions of hebephrenic and paranoid schizophrenics, and of the two groups combined, in an Italian population and in a combined group from the Swedish population have been analyzed statistically. There is a significantly decreased frequency of HLA-A10 in all of these. Theae are some preliminary indications of an increased frequency (a positive association) for some of the other antigens of the HLA-SD series, but there is insufficient data at present for evaluating the significance of these findings. Differences between hebephrenic and paranoid schizophrenics have been detected.", "contents": "HLA-SD antigens and schizophrenia: statistical and genetical considerations. The HLA-SD phenotype distributions of hebephrenic and paranoid schizophrenics, and of the two groups combined, in an Italian population and in a combined group from the Swedish population have been analyzed statistically. There is a significantly decreased frequency of HLA-A10 in all of these. Theae are some preliminary indications of an increased frequency (a positive association) for some of the other antigens of the HLA-SD series, but there is insufficient data at present for evaluating the significance of these findings. Differences between hebephrenic and paranoid schizophrenics have been detected.", "PMID": 973210}
{"id": "pubmed23n0032_9200", "title": "HLA haplotypes in a genetic isolate in Newfoundland. A population showing 8% homozygosity and a familial aggregate of lymphoma and immunodeficiency cases.", "content": "HLA typing was performed on 384 individuals of an isolated population of 1,500 people with a familial aggregate of lymphoma and immunodeficiency cases. Eighty-five % of the total population were descendants of the founding couple. First cousin marriages were common. There was a three-fold or higher increase of the following haplotypes as compared to the frequencies in Sheffield: HLA-A28,Bw35, HLA-A28, B18, HLA-A10, B18, HLA-A2, B18,HLA-A11, Bw40 and HLA-A11, B7. The frequency of HLA-A1, B8 was low (5.4%). The most common genotype was HLA-A2, B12/A2, B12 followed by HLA-A2, B12/A28, Bw35. We found 20 HLA homozygous individuals, of these 15 were HLA-A2, b12/a2, b12. There were two possible HLA cross-overs which may be confirmed and three postulated cross-overs which can never be confirmed as one or both parents of the individuals in question are deceased. Some of the haplotypes could be traced back to the first, second and third generations, i.e. to the first half of the nineteenth century. No single haplotype or antigen was shared by the patients.", "contents": "HLA haplotypes in a genetic isolate in Newfoundland. A population showing 8% homozygosity and a familial aggregate of lymphoma and immunodeficiency cases. HLA typing was performed on 384 individuals of an isolated population of 1,500 people with a familial aggregate of lymphoma and immunodeficiency cases. Eighty-five % of the total population were descendants of the founding couple. First cousin marriages were common. There was a three-fold or higher increase of the following haplotypes as compared to the frequencies in Sheffield: HLA-A28,Bw35, HLA-A28, B18, HLA-A10, B18, HLA-A2, B18,HLA-A11, Bw40 and HLA-A11, B7. The frequency of HLA-A1, B8 was low (5.4%). The most common genotype was HLA-A2, B12/A2, B12 followed by HLA-A2, B12/A28, Bw35. We found 20 HLA homozygous individuals, of these 15 were HLA-A2, b12/a2, b12. There were two possible HLA cross-overs which may be confirmed and three postulated cross-overs which can never be confirmed as one or both parents of the individuals in question are deceased. Some of the haplotypes could be traced back to the first, second and third generations, i.e. to the first half of the nineteenth century. No single haplotype or antigen was shared by the patients.", "PMID": 973211}
{"id": "pubmed23n0032_9201", "title": "Metabolism of testosterone by the epididymis and ventral prostate of rat and its inhibition by cyproterone acetate.", "content": "The metabolism of 3H-testosterone by the epididymis and accessory organs of adult male rats exposed continuously to microdoses of cyproterone acetate from subcutaneous capsules were studied. The major metabolite of 3H-testosterone in the epididymis, vas deferens and ventral prostate of control rat was dihydrotestosterone while the formation of androstanediol by these tissues was low. The highest percentage of DHT was formed by the ventral prostate and cauda epididymis. In rats exposed to cyproterone acetate for four months, the conversion of testosterone to DHT was inhibited in all the tissues but maximally in the ventral prostate and cauda epididymis. In these rats, the secretory function of the ventral prostate was normal while that of the epididymis was markedly decreased. These data are discussed based on the differential thresholds of androgens required to regulate the functions of the accessory organs.", "contents": "Metabolism of testosterone by the epididymis and ventral prostate of rat and its inhibition by cyproterone acetate. The metabolism of 3H-testosterone by the epididymis and accessory organs of adult male rats exposed continuously to microdoses of cyproterone acetate from subcutaneous capsules were studied. The major metabolite of 3H-testosterone in the epididymis, vas deferens and ventral prostate of control rat was dihydrotestosterone while the formation of androstanediol by these tissues was low. The highest percentage of DHT was formed by the ventral prostate and cauda epididymis. In rats exposed to cyproterone acetate for four months, the conversion of testosterone to DHT was inhibited in all the tissues but maximally in the ventral prostate and cauda epididymis. In these rats, the secretory function of the ventral prostate was normal while that of the epididymis was markedly decreased. These data are discussed based on the differential thresholds of androgens required to regulate the functions of the accessory organs.", "PMID": 973232}
{"id": "pubmed23n0032_9202", "title": "Mass spectral fragmentation of 18-norsteroids and 12-oxo-20-hydroxysteroids.", "content": "Comparison of the mass spectra of 18-norsteroids with those of the corresponding normal steroids revealed that the absence of the 18-methyl group has little effect on the fragmentation pattern, especially on the D-ring and side-chain cleavage. The results indicate that the stability-of the cation or radical species, due to the quarternary nature of C-13, is not a major driving force in the key 13-17 cleavage. Hydrogen transfer observed in the D-ring fragmentation of 20-hydroxy-18-nor-5 alpha-pregnan-13-ones was not found to be specific to the 18-norcompounds. Deuterium labeling at the 20-OH and 20 alpha-H has suggested the involvement of a unique triple hydrogen rearrangement in this major cleavage. Migration of the 20-hydrogen to the 12-keto oxygen was also observed in the fragmentation of dammarane derivatives and was likewise confirmed by deuterium labeling.", "contents": "Mass spectral fragmentation of 18-norsteroids and 12-oxo-20-hydroxysteroids. Comparison of the mass spectra of 18-norsteroids with those of the corresponding normal steroids revealed that the absence of the 18-methyl group has little effect on the fragmentation pattern, especially on the D-ring and side-chain cleavage. The results indicate that the stability-of the cation or radical species, due to the quarternary nature of C-13, is not a major driving force in the key 13-17 cleavage. Hydrogen transfer observed in the D-ring fragmentation of 20-hydroxy-18-nor-5 alpha-pregnan-13-ones was not found to be specific to the 18-norcompounds. Deuterium labeling at the 20-OH and 20 alpha-H has suggested the involvement of a unique triple hydrogen rearrangement in this major cleavage. Migration of the 20-hydrogen to the 12-keto oxygen was also observed in the fragmentation of dammarane derivatives and was likewise confirmed by deuterium labeling.", "PMID": 973233}
{"id": "pubmed23n0032_9203", "title": "A simple method for the assay of eight steroids in small volumes of plasma.", "content": "A simple method is described for the simultaneous radioligand assay of four delta5-3beta-hydroxysteroids adjacent to one another on the biosynthetic pathway (pregnenolone [1], 17alpha-hydroxypregnenolone, dehydroepiandrosterone and 5-androsterone-3beta, 17beta-diol), and their four delta4-3keto products (progesterone, 17alpha-hydroxyprogesterone, 4-androstene-3, 17-dione and testosterone). Two plasma aliquots are extracted and fractionated each for four steroids and individual corrections are made for losses. For fractionation, maximum use is made of the high resolution and reproducibility of celite minicolumns, using propylene glycol as stationary phase, and a discontinuous gradient of ethyl acetate in iso-octane as mobile phase. The fractions are then assayed in the appropriate radioligand end-assay system. Each assay was finally validated by demonstrating coincidence of peaks of immuno- and radioactive steroid in extracts of female plasma. Results in pre-pubertal girls and women in the follicular phase of the menstrual cycle suggest that the major change in adrenal steroid production at puberty may be an increase in 17, 20-desmolase activity. There appears to be little reversal of this change in adrenal function after ovariectomy.", "contents": "A simple method for the assay of eight steroids in small volumes of plasma. A simple method is described for the simultaneous radioligand assay of four delta5-3beta-hydroxysteroids adjacent to one another on the biosynthetic pathway (pregnenolone [1], 17alpha-hydroxypregnenolone, dehydroepiandrosterone and 5-androsterone-3beta, 17beta-diol), and their four delta4-3keto products (progesterone, 17alpha-hydroxyprogesterone, 4-androstene-3, 17-dione and testosterone). Two plasma aliquots are extracted and fractionated each for four steroids and individual corrections are made for losses. For fractionation, maximum use is made of the high resolution and reproducibility of celite minicolumns, using propylene glycol as stationary phase, and a discontinuous gradient of ethyl acetate in iso-octane as mobile phase. The fractions are then assayed in the appropriate radioligand end-assay system. Each assay was finally validated by demonstrating coincidence of peaks of immuno- and radioactive steroid in extracts of female plasma. Results in pre-pubertal girls and women in the follicular phase of the menstrual cycle suggest that the major change in adrenal steroid production at puberty may be an increase in 17, 20-desmolase activity. There appears to be little reversal of this change in adrenal function after ovariectomy.", "PMID": 973234}
{"id": "pubmed23n0032_9204", "title": "Antiestrogen action: differential nuclear retention and extractability of the estrogen receptor.", "content": "Since there is a much longer uterine nuclear retention of the U-11, 100A (antiestrogen) receptor complex (UARC) than of the estradiol receptor complex (ERC) at 4-12 hrs after injection, experiments were designed to determine if there is a difference between the relative nuclear affinities for the two RCs as determined by extraction with various ionic strength mediums. Although the UARC was retained longer in the nuclear fraction in vivo, the UARC was completely extractable with 0.3M KCl or 50mM spermine, whereas the ERC demonstrates a salt-resistant form. This suggests that the ERC is more tightly bound to nuclear components through this salt-resistant form of the receptor. In addition, various intercalating agents were used to distinguish the different nuclear chromatin DNA sites where the UARC and ERC may be binding. With actinomycin D (50 uM) more ERC than UARC was retained in the nuclear fraction. However, with ethidium bromide (100uM) less ERC than UARC was retained. Also, the ERC selectively released by ethidium bromide is precisely that fraction not released by salt. These results indicate that the UARC and ERC bind to different chromatin loci.", "contents": "Antiestrogen action: differential nuclear retention and extractability of the estrogen receptor. Since there is a much longer uterine nuclear retention of the U-11, 100A (antiestrogen) receptor complex (UARC) than of the estradiol receptor complex (ERC) at 4-12 hrs after injection, experiments were designed to determine if there is a difference between the relative nuclear affinities for the two RCs as determined by extraction with various ionic strength mediums. Although the UARC was retained longer in the nuclear fraction in vivo, the UARC was completely extractable with 0.3M KCl or 50mM spermine, whereas the ERC demonstrates a salt-resistant form. This suggests that the ERC is more tightly bound to nuclear components through this salt-resistant form of the receptor. In addition, various intercalating agents were used to distinguish the different nuclear chromatin DNA sites where the UARC and ERC may be binding. With actinomycin D (50 uM) more ERC than UARC was retained in the nuclear fraction. However, with ethidium bromide (100uM) less ERC than UARC was retained. Also, the ERC selectively released by ethidium bromide is precisely that fraction not released by salt. These results indicate that the UARC and ERC bind to different chromatin loci.", "PMID": 973236}
{"id": "pubmed23n0032_9205", "title": "In vivo and in vitro studies of 15alpha-hydroxyprogesterone in the human placenta.", "content": "Studies were conducted to determine the fate of 15alpha-hydroxyprogesterone in human placental tissue. Tritiated 15alpha-hydroxyprogesterone was perfused through normal human placentas in situ at the time of Cesarean section and incubated with a 10,000x g microsomal supernate of the placenta in vitro. In both systems the substrate, but no additional metabolites were identified. These findings indicate that 15alpha-hydroxyprogesterone is not metabolized during its passage in the human term placenta, and suggests that because of its fetal origin clinical measurements of 15alpha-hydroxyprogesterone may provide a valuable index to the status of fetal viability.", "contents": "In vivo and in vitro studies of 15alpha-hydroxyprogesterone in the human placenta. Studies were conducted to determine the fate of 15alpha-hydroxyprogesterone in human placental tissue. Tritiated 15alpha-hydroxyprogesterone was perfused through normal human placentas in situ at the time of Cesarean section and incubated with a 10,000x g microsomal supernate of the placenta in vitro. In both systems the substrate, but no additional metabolites were identified. These findings indicate that 15alpha-hydroxyprogesterone is not metabolized during its passage in the human term placenta, and suggests that because of its fetal origin clinical measurements of 15alpha-hydroxyprogesterone may provide a valuable index to the status of fetal viability.", "PMID": 973237}
{"id": "pubmed23n0032_9206", "title": "Steroid aromatization by a small particle fraction from porcine ovaries.", "content": "Testosterone, 4-androstene-3,17-dione, 19-hydroxy-4-androstene-3,17-dione, 4alpha-5-oxido-5alpha-androstane-3,17-dione and 4beta-5-oxido-5beta-androstane-3,17-dione were compared as substrates for aromatization by the small particle fraction from sow ovaries. Relative conversion rates were: 19-hydroxy-4-androstene-3,17-dione:4-androstene-3,17-dione: testosterone: 4alpha-5-oxido-5alpha- and 4beta-5-oxido-5beta-androstane-3,17-dione; 100:32:20:congruent to O. Apparent Michaelis constants were 4.4 muM for 4-androstene-3,17-dione and 12 muM for testosterone. Maximum velocities were 0.20 pmoles/mg protein per min for androstenedione [1] and 0.16 for testosterone. The substrate preferences of the aromatizing system found in ovary are similar to those of the enzyme found in placenta.", "contents": "Steroid aromatization by a small particle fraction from porcine ovaries. Testosterone, 4-androstene-3,17-dione, 19-hydroxy-4-androstene-3,17-dione, 4alpha-5-oxido-5alpha-androstane-3,17-dione and 4beta-5-oxido-5beta-androstane-3,17-dione were compared as substrates for aromatization by the small particle fraction from sow ovaries. Relative conversion rates were: 19-hydroxy-4-androstene-3,17-dione:4-androstene-3,17-dione: testosterone: 4alpha-5-oxido-5alpha- and 4beta-5-oxido-5beta-androstane-3,17-dione; 100:32:20:congruent to O. Apparent Michaelis constants were 4.4 muM for 4-androstene-3,17-dione and 12 muM for testosterone. Maximum velocities were 0.20 pmoles/mg protein per min for androstenedione [1] and 0.16 for testosterone. The substrate preferences of the aromatizing system found in ovary are similar to those of the enzyme found in placenta.", "PMID": 973238}
{"id": "pubmed23n0032_9207", "title": "Characterization of the androgen receptor in the skeletal muscle of the rat.", "content": "Skeletal muscle homogenate of prepuberal, adult uncastrated or short-(1-4days) or long-term (20days) castrated male and female rats was incubated at 0degreesC iwth highly labeled (3H)5alpha-dihydrotestosterone (1) or (3H) testosterone. labeled (3H)5alpha-dihydrotestosterone (1) or 3H) testosterone. After incubation bound and free hormone in the 100,000 g cytosol were separated by agargel electrophoresis at low temperature. Furthermore, the percentage distribution of the main metabolites found in the cytosol was analyzed by thin-layer chromatography. Two androgen binding proteins could be found: One with high affinity (apparent dissociation and constant (Kd)= 1.4 - 6.4X10(-9)M) low capacity (receptor), the other with relative low affinity high capacity. The physico-chemical characteristics of the androgen receptor in the rat skeletal muscle cytosol are similar if not identical to those which have been described for the androgen receptor in the prostate and other androgen dependent organs. However, the amount of available 5alpha-DHT-binding sites in the skeletal muscle is about 60 times and 7 times lower than in the prostate and bulbocavernosus/levator ani muscle, respectively. No essential androgen metabolism occurs in vitro at 0degreesC in the skeletal muscle.", "contents": "Characterization of the androgen receptor in the skeletal muscle of the rat. Skeletal muscle homogenate of prepuberal, adult uncastrated or short-(1-4days) or long-term (20days) castrated male and female rats was incubated at 0degreesC iwth highly labeled (3H)5alpha-dihydrotestosterone (1) or (3H) testosterone. labeled (3H)5alpha-dihydrotestosterone (1) or 3H) testosterone. After incubation bound and free hormone in the 100,000 g cytosol were separated by agargel electrophoresis at low temperature. Furthermore, the percentage distribution of the main metabolites found in the cytosol was analyzed by thin-layer chromatography. Two androgen binding proteins could be found: One with high affinity (apparent dissociation and constant (Kd)= 1.4 - 6.4X10(-9)M) low capacity (receptor), the other with relative low affinity high capacity. The physico-chemical characteristics of the androgen receptor in the rat skeletal muscle cytosol are similar if not identical to those which have been described for the androgen receptor in the prostate and other androgen dependent organs. However, the amount of available 5alpha-DHT-binding sites in the skeletal muscle is about 60 times and 7 times lower than in the prostate and bulbocavernosus/levator ani muscle, respectively. No essential androgen metabolism occurs in vitro at 0degreesC in the skeletal muscle.", "PMID": 973239}
{"id": "pubmed23n0032_9208", "title": "Synthesis and purification of radioactive 6beta-iodomethyl-19-norcholest-5(10)-en-3beta-ol.", "content": "A method for the synthesis and purification of 6beta-iodomethyl-19-norcholest-5(10)-ene-3beta-ol-131I of greater than 98 mole% chemical purity and greater than 99% radiochemical purity is presented. Carbon-13 and proton NMR were used to establish the identity and purity. Discrepancies in the characterization of this compound, previously published by two other research groups, are discussed.", "contents": "Synthesis and purification of radioactive 6beta-iodomethyl-19-norcholest-5(10)-en-3beta-ol. A method for the synthesis and purification of 6beta-iodomethyl-19-norcholest-5(10)-ene-3beta-ol-131I of greater than 98 mole% chemical purity and greater than 99% radiochemical purity is presented. Carbon-13 and proton NMR were used to establish the identity and purity. Discrepancies in the characterization of this compound, previously published by two other research groups, are discussed.", "PMID": 973240}
{"id": "pubmed23n0032_9209", "title": "Toxoplasmin skin sensitivity in Calcutta area.", "content": "Toxoplasmin skin test was performed on 574 suspected and 24 control cases. A positive reaction was noted in 92 of 481 ophthalmological cases; two of 17 lymphadenopathy cases; none in 36 cerebral palsy cases and four of 40 miscellaneous cases comprising pyrexia of unknown aetiology, recurrent abortions etc. None from the control group gave a positive reaction. The significance of the results is discussed and the need for further studies employing other investigative methods is stressed.", "contents": "Toxoplasmin skin sensitivity in Calcutta area. Toxoplasmin skin test was performed on 574 suspected and 24 control cases. A positive reaction was noted in 92 of 481 ophthalmological cases; two of 17 lymphadenopathy cases; none in 36 cerebral palsy cases and four of 40 miscellaneous cases comprising pyrexia of unknown aetiology, recurrent abortions etc. None from the control group gave a positive reaction. The significance of the results is discussed and the need for further studies employing other investigative methods is stressed.", "PMID": 973246}
{"id": "pubmed23n0032_9210", "title": "Pulmonary oedema associated with acidosis in patients with cholera.", "content": "Five patients with severe acidosis and pulmonary oedema complicating cholera were seen at the Cholera Research Laboratory, Dacca, in a two-year period. All had had inadequate treatment. Their disease resulted in acidosis prior to admission; only the two who subsequently survived received volumes of sodium bicarbonate solutions sufficiently large to repair completely their acidosis. Saline alone worsened pulmonary congestion, while alkali appeared to relieve it despite the accompanying volume expansion. These observations are consistent with the known redistribution of blood to the central circulation in acidosis. Timely and proper treatment of cholera will avert this syndrome, when use of isotonic sodium bicarbonate sufficient to correct acidosis may be very helpful.", "contents": "Pulmonary oedema associated with acidosis in patients with cholera. Five patients with severe acidosis and pulmonary oedema complicating cholera were seen at the Cholera Research Laboratory, Dacca, in a two-year period. All had had inadequate treatment. Their disease resulted in acidosis prior to admission; only the two who subsequently survived received volumes of sodium bicarbonate solutions sufficiently large to repair completely their acidosis. Saline alone worsened pulmonary congestion, while alkali appeared to relieve it despite the accompanying volume expansion. These observations are consistent with the known redistribution of blood to the central circulation in acidosis. Timely and proper treatment of cholera will avert this syndrome, when use of isotonic sodium bicarbonate sufficient to correct acidosis may be very helpful.", "PMID": 973248}
{"id": "pubmed23n0032_9211", "title": "Blood phenylalanine levels in mentally retarded African children: a study of 138 patients from Ibadan, Nigeria.", "content": "Blood phenylalanine levels were determined by the Guthrie microbiological inhibition technique in 138 mentally retarded, and in 88 randomly selected normal (control) Nigerian children. All the control subjects and 137 of the 138 mentally retarded children had negative Guthrie tests (6 mg/100 ml or less). In a majority of the children, the blood phenylalanine levels were 2 mg/100 ml or less. One mentally retarded child had moderately elevated blood phenylalanine level which was however much lower than the levels associated with classical phenylketonuria. The findings in the present study is in agreement with previously reported rarity of phenylketonuria in American negroes. An extensive survey of tropical Africa for phenylketonuria is, however, still desirable because regional and ethnic differences in the incidence of the disease may exist.", "contents": "Blood phenylalanine levels in mentally retarded African children: a study of 138 patients from Ibadan, Nigeria. Blood phenylalanine levels were determined by the Guthrie microbiological inhibition technique in 138 mentally retarded, and in 88 randomly selected normal (control) Nigerian children. All the control subjects and 137 of the 138 mentally retarded children had negative Guthrie tests (6 mg/100 ml or less). In a majority of the children, the blood phenylalanine levels were 2 mg/100 ml or less. One mentally retarded child had moderately elevated blood phenylalanine level which was however much lower than the levels associated with classical phenylketonuria. The findings in the present study is in agreement with previously reported rarity of phenylketonuria in American negroes. An extensive survey of tropical Africa for phenylketonuria is, however, still desirable because regional and ethnic differences in the incidence of the disease may exist.", "PMID": 973249}
{"id": "pubmed23n0032_9212", "title": "Stimulation of healing of varicose ulcers by ultrasound.", "content": "The local application of theraputic ultrasound has been shown, in a controlled trial, to stimulate the healing of chronic varicose ulcers. The change in ulcer size by the end of the period of treatment was noted. It was found that healing of insonated ulcers was significantly more marked than that exhibited by the controls.", "contents": "Stimulation of healing of varicose ulcers by ultrasound. The local application of theraputic ultrasound has been shown, in a controlled trial, to stimulate the healing of chronic varicose ulcers. The change in ulcer size by the end of the period of treatment was noted. It was found that healing of insonated ulcers was significantly more marked than that exhibited by the controls.", "PMID": 973258}
{"id": "pubmed23n0032_9213", "title": "[The double-pyelon kidney with special regard to the transport function (author's transl)].", "content": "The double-pyelon kidney, i.e. the total or partial duplication of the urinary transport system may involve both diagnostic and therapeutic difficulties. Observations are presented on the strength of 398 cases with total or partial duplication of the upper urinary tract. The incidence rate was 0.7 percent. The various types of duplication are presented with the diagnostic and therapeutic features involved. Transport function is discussed in detail as investigated under ideal physiologic conditions: during excretory urography under the amplifier screen. Aiming at early detection of the congenital anomaly and specification of the appropriate management, a urologic check-up is suggested even with mild presenting symptoms. Special emphasis is given to the consistent day-time enuresis in children over 3 years old, as a possible sign of ectopic ureter. Both children and parents are saved much unpleasantness by early detection, proper management and considerate surgery.", "contents": "[The double-pyelon kidney with special regard to the transport function (author's transl)]. The double-pyelon kidney, i.e. the total or partial duplication of the urinary transport system may involve both diagnostic and therapeutic difficulties. Observations are presented on the strength of 398 cases with total or partial duplication of the upper urinary tract. The incidence rate was 0.7 percent. The various types of duplication are presented with the diagnostic and therapeutic features involved. Transport function is discussed in detail as investigated under ideal physiologic conditions: during excretory urography under the amplifier screen. Aiming at early detection of the congenital anomaly and specification of the appropriate management, a urologic check-up is suggested even with mild presenting symptoms. Special emphasis is given to the consistent day-time enuresis in children over 3 years old, as a possible sign of ectopic ureter. Both children and parents are saved much unpleasantness by early detection, proper management and considerate surgery.", "PMID": 973275}
{"id": "pubmed23n0032_9214", "title": "[Vesicoureterorenal reflux: an inherited disease? (author's transl)].", "content": "A family case history is presented with an increased incidence of vesicorenal reflux: four sons had a reflux while a daughter and the parents were healthy in this respect. From the literature and on own studies the possibility of reflux as an inherited disease is discussed. A new finding is the coincidence of reflux and the histocompatibility-antigen HLA-A2.", "contents": "[Vesicoureterorenal reflux: an inherited disease? (author's transl)]. A family case history is presented with an increased incidence of vesicorenal reflux: four sons had a reflux while a daughter and the parents were healthy in this respect. From the literature and on own studies the possibility of reflux as an inherited disease is discussed. A new finding is the coincidence of reflux and the histocompatibility-antigen HLA-A2.", "PMID": 973276}
{"id": "pubmed23n0032_9215", "title": "[Congenital bllbar urethral stenosis and its therapy by means of internal urethrotomy under direct vision (according to Sachse) (author's transl)].", "content": "Congenital bulbar urethral stenosis arises from incomplete opening of the urogenital membrane. The etiology may possibly be genetic or else a disturbance in the maternal hormones during the embryonic phase. The diagnosis from other types of stenosis is made by urethroscopy. The urodynamic consequences are a compensatory hypertrophy of bladder muscle, in extreme cases finally followed by hydronephrosis. Symptoms of stenosis include dysuria, polyuria, enuresis, and recurrent urinary tract infection. The whole urinary tract should be investigated. At present, the operation of choice for dealing with this type of stenosis, is internal urethrotomy under direct vision (Sachse's operation). Short-term indwelling catheterization, prophylaxis against infection, and hydraulic auto-bougination (raising the internal urethral pressure by compressing the penis during micturition) help to ensure success. The percentage of complications is low. The risk of recurrence is less than with the use of electrocautery.", "contents": "[Congenital bllbar urethral stenosis and its therapy by means of internal urethrotomy under direct vision (according to Sachse) (author's transl)]. Congenital bulbar urethral stenosis arises from incomplete opening of the urogenital membrane. The etiology may possibly be genetic or else a disturbance in the maternal hormones during the embryonic phase. The diagnosis from other types of stenosis is made by urethroscopy. The urodynamic consequences are a compensatory hypertrophy of bladder muscle, in extreme cases finally followed by hydronephrosis. Symptoms of stenosis include dysuria, polyuria, enuresis, and recurrent urinary tract infection. The whole urinary tract should be investigated. At present, the operation of choice for dealing with this type of stenosis, is internal urethrotomy under direct vision (Sachse's operation). Short-term indwelling catheterization, prophylaxis against infection, and hydraulic auto-bougination (raising the internal urethral pressure by compressing the penis during micturition) help to ensure success. The percentage of complications is low. The risk of recurrence is less than with the use of electrocautery.", "PMID": 973277}
{"id": "pubmed23n0032_9216", "title": "[Complete and incomplete urethra duplications (author's transl)].", "content": "With the help of four cases of urethra duplications, a report is presented on the occurrence, symptomatology, diagnostics, and therapy of the clinical picture. The developmental anatomy is dealt with briefly.", "contents": "[Complete and incomplete urethra duplications (author's transl)]. With the help of four cases of urethra duplications, a report is presented on the occurrence, symptomatology, diagnostics, and therapy of the clinical picture. The developmental anatomy is dealt with briefly.", "PMID": 973278}
{"id": "pubmed23n0032_9217", "title": "[Embryonal rhabdomyosarcoma of the urinary bladder in childhood. Report of five own cases].", "content": "Malignant lesions of the bladder in infancy and early childhood arise from mesenchymal tissue. Among the mesenchymal tumors embryonal rhabdomyosarcoma is more common in young children than in adults. The gross appearance of rhabdomyosarcoma of the bladder is typically that of polypoid grapelike clusters of tissue, termed \"sarcoma botryoides\". Histologically, the lesion is consistent with embryonal rhabdomyosarcoma. The growths originate in the submucosal layer of the trigone and the internal urethral orifice. Rhabdomyosarcoma (RMS) of the bladder in children tends to remain within the disease. Deep extension and metastasis to regional lymph nodes and to distant sites is a relatively late occurrence. The symptoms are characteristically those of obstruction and strangury. Hematuris is uncommon. The intravenous urogram shows characteristic lobulated filling defects. Treatment of choice is almost always total cystectomy. Suprapubic cystotomy must be prohibited both as a method of drainage and as an approach for biopsy since tumors ulcerating through the abdominal wall after cystotomy and local excision have been observed. With concomitant administration of radiation therapy in selected cases and the combination of surgery with multiple drug chemotherapy, promising results have been obtained in the therapy of embryonal RMS of the bladder in children.", "contents": "[Embryonal rhabdomyosarcoma of the urinary bladder in childhood. Report of five own cases]. Malignant lesions of the bladder in infancy and early childhood arise from mesenchymal tissue. Among the mesenchymal tumors embryonal rhabdomyosarcoma is more common in young children than in adults. The gross appearance of rhabdomyosarcoma of the bladder is typically that of polypoid grapelike clusters of tissue, termed \"sarcoma botryoides\". Histologically, the lesion is consistent with embryonal rhabdomyosarcoma. The growths originate in the submucosal layer of the trigone and the internal urethral orifice. Rhabdomyosarcoma (RMS) of the bladder in children tends to remain within the disease. Deep extension and metastasis to regional lymph nodes and to distant sites is a relatively late occurrence. The symptoms are characteristically those of obstruction and strangury. Hematuris is uncommon. The intravenous urogram shows characteristic lobulated filling defects. Treatment of choice is almost always total cystectomy. Suprapubic cystotomy must be prohibited both as a method of drainage and as an approach for biopsy since tumors ulcerating through the abdominal wall after cystotomy and local excision have been observed. With concomitant administration of radiation therapy in selected cases and the combination of surgery with multiple drug chemotherapy, promising results have been obtained in the therapy of embryonal RMS of the bladder in children.", "PMID": 973279}
{"id": "pubmed23n0032_9218", "title": "[Carcinoma of the bladder in childhood (author's transl)].", "content": "The authors describe the extremely rare finding of a keratinized squamous cell carcinoma of the bladder in a 13 year old girl without however a history of the typical symptoms of the illness such as hematuria. The findings are reviewed in the light of the current literature. Enuresis leading to a chronic infection of the urinary tract is discussed as the possible etiologic factor.", "contents": "[Carcinoma of the bladder in childhood (author's transl)]. The authors describe the extremely rare finding of a keratinized squamous cell carcinoma of the bladder in a 13 year old girl without however a history of the typical symptoms of the illness such as hematuria. The findings are reviewed in the light of the current literature. Enuresis leading to a chronic infection of the urinary tract is discussed as the possible etiologic factor.", "PMID": 973280}
{"id": "pubmed23n0032_9219", "title": "[The controlled TUR: a new method for the measurement of intraoperative blood-loss during TUR (author's transl)].", "content": "The measurement of intraoperative blood-loss during TUR is concerned as a compulsory procedure for this kind of operation. After testing so far known methods for measuring blood-volume in blood-water-mixtures and demonstrating greater disadvantages of these methods, we developed a new photometer, which allows an immediate and exact estimation of the blood-loss. We compared this new method with other also exact but more complicated methods, had laboratory tests and tests in the daily operative routine and excluded possible mistakes.", "contents": "[The controlled TUR: a new method for the measurement of intraoperative blood-loss during TUR (author's transl)]. The measurement of intraoperative blood-loss during TUR is concerned as a compulsory procedure for this kind of operation. After testing so far known methods for measuring blood-volume in blood-water-mixtures and demonstrating greater disadvantages of these methods, we developed a new photometer, which allows an immediate and exact estimation of the blood-loss. We compared this new method with other also exact but more complicated methods, had laboratory tests and tests in the daily operative routine and excluded possible mistakes.", "PMID": 973281}
{"id": "pubmed23n0032_9220", "title": "Anterior transperitoneal approach for removal of renal stones.", "content": "An anterior transperitoneal approach was used for removal of stones from 15 kidneys in 13 patients. Exposure of the anterior portion of the pelvis was readily accomplished making excellent exposure of the interior of the kidney possible. Minimal urinary drainage was noted postoperatively. The operative procedures done with the patients in the supine position were well tolerated.", "contents": "Anterior transperitoneal approach for removal of renal stones. An anterior transperitoneal approach was used for removal of stones from 15 kidneys in 13 patients. Exposure of the anterior portion of the pelvis was readily accomplished making excellent exposure of the interior of the kidney possible. Minimal urinary drainage was noted postoperatively. The operative procedures done with the patients in the supine position were well tolerated.", "PMID": 973284}
{"id": "pubmed23n0032_9221", "title": "Dissolution of uric acid stones: alternative to surgery.", "content": "A method for the dissolution of uric acid urolithiasis by irrigation with sodium bicarbonate solution through a ureteral catheter is described.", "contents": "Dissolution of uric acid stones: alternative to surgery. A method for the dissolution of uric acid urolithiasis by irrigation with sodium bicarbonate solution through a ureteral catheter is described.", "PMID": 973285}
{"id": "pubmed23n0032_9222", "title": "Doxorubicin chemotherapy in advanced transitional cell carcinoma.", "content": "During the past year 25 patients with advanced transitional cell carcinoma were treated with intravenous doxorubicin hydrochloride (Adriamycin), 60 to 75 mg. per square meter of body surface area, every three weeks. Among the 19 evaluable patients, one partial objective remission lasting five months was observed. All 7 patients with bone pain had symptomatic relief and 12 patients had significant subjective improvement lasting an average of six-and-a-half months. Side effects were minimal and consisted of alopecia, mild leukopenia, and mild stomatitis; no significant cardiotoxicity was observed. Doxorubicin hydrochloride appears to have important antitumor activity in advanced urothelial tumors. Controlled clinical trials with this agent alone and in combined drug regimens are needed.", "contents": "Doxorubicin chemotherapy in advanced transitional cell carcinoma. During the past year 25 patients with advanced transitional cell carcinoma were treated with intravenous doxorubicin hydrochloride (Adriamycin), 60 to 75 mg. per square meter of body surface area, every three weeks. Among the 19 evaluable patients, one partial objective remission lasting five months was observed. All 7 patients with bone pain had symptomatic relief and 12 patients had significant subjective improvement lasting an average of six-and-a-half months. Side effects were minimal and consisted of alopecia, mild leukopenia, and mild stomatitis; no significant cardiotoxicity was observed. Doxorubicin hydrochloride appears to have important antitumor activity in advanced urothelial tumors. Controlled clinical trials with this agent alone and in combined drug regimens are needed.", "PMID": 973286}
{"id": "pubmed23n0032_9223", "title": "Xenon-133 resorption in urinary bladder: functional diagnosis of bladder epithelium.", "content": "The examination of resorption from the urinary bladder had previously been restricted almost exclusively to animal research, since, as a rule, a separation of the bladder from the urinary tract is required. If this is not done, the resorbed test substance quickly finds its way back into the bladder. More than 95 per cent of the xenon which has been resorbed from the bladder becomes eliminated through the lungs. The detection and measurement of xenon in the exhaled air enables one to conduct, in a relatively simple fashion, urinary bladder resorption research using human subjects. Studies based on 141 patients showed that the xenon exhalation tests currently represent the most sensitive and exact method for detecting and determining the nature of inflammatory diseases of the urinary bladder. With a number of so-called \"irritable bladder\" cases it became clear that functional disorders of the bladder epithelium could be present without any evidence of associated morphologic changes. With radiogenic treatment of tumors in the lower pelvic region one experiences an increase in bladder resorption with increased exposure to radiation. There exists, as it were, a linear correlation between the radiation dosage and the degree of resorption from the urinary bladder. Three months after terminating radiotherapy one can detect, as a rule, only a negligible increase in resorption. If at this time the rate of xenon resorption still remains clearly high, one must reckon with permanent radiation damage of the bladder, insofar as it proves unsuccessful to eliminate the cause of cystitis.", "contents": "Xenon-133 resorption in urinary bladder: functional diagnosis of bladder epithelium. The examination of resorption from the urinary bladder had previously been restricted almost exclusively to animal research, since, as a rule, a separation of the bladder from the urinary tract is required. If this is not done, the resorbed test substance quickly finds its way back into the bladder. More than 95 per cent of the xenon which has been resorbed from the bladder becomes eliminated through the lungs. The detection and measurement of xenon in the exhaled air enables one to conduct, in a relatively simple fashion, urinary bladder resorption research using human subjects. Studies based on 141 patients showed that the xenon exhalation tests currently represent the most sensitive and exact method for detecting and determining the nature of inflammatory diseases of the urinary bladder. With a number of so-called \"irritable bladder\" cases it became clear that functional disorders of the bladder epithelium could be present without any evidence of associated morphologic changes. With radiogenic treatment of tumors in the lower pelvic region one experiences an increase in bladder resorption with increased exposure to radiation. There exists, as it were, a linear correlation between the radiation dosage and the degree of resorption from the urinary bladder. Three months after terminating radiotherapy one can detect, as a rule, only a negligible increase in resorption. If at this time the rate of xenon resorption still remains clearly high, one must reckon with permanent radiation damage of the bladder, insofar as it proves unsuccessful to eliminate the cause of cystitis.", "PMID": 973287}
{"id": "pubmed23n0032_9224", "title": "Effect of preoperative antibiotic therapy on bacterial prostatitis after transurethral prostatectomy.", "content": "Transurethral prostatectomy was performed on 237 patients who required no preoperative antimicrobial therapy and on 182 patients with symptoms of urinary tract infection who received preoperative antimicrobial therapy. At operation all patients were asymptomatic. Data are presented on the incidence of infected prostates, bacteriuria at operation, and postoperative morbidity for the two groups. The findings refute the concept that the chronically infected prostate is resistant to antimicrobial therapy. Transurethral prostatectomy in an infected field was found to increase morbidity. The data suggest that an appropriate preoperative antimicrobial regimen be administered to patients undergoing transurethral prostatectomy with asymptomatic bacteriuria.", "contents": "Effect of preoperative antibiotic therapy on bacterial prostatitis after transurethral prostatectomy. Transurethral prostatectomy was performed on 237 patients who required no preoperative antimicrobial therapy and on 182 patients with symptoms of urinary tract infection who received preoperative antimicrobial therapy. At operation all patients were asymptomatic. Data are presented on the incidence of infected prostates, bacteriuria at operation, and postoperative morbidity for the two groups. The findings refute the concept that the chronically infected prostate is resistant to antimicrobial therapy. Transurethral prostatectomy in an infected field was found to increase morbidity. The data suggest that an appropriate preoperative antimicrobial regimen be administered to patients undergoing transurethral prostatectomy with asymptomatic bacteriuria.", "PMID": 973288}
{"id": "pubmed23n0032_9225", "title": "Prostatic cancer: evolution of treatment at a comprehensive center (1970-1974).", "content": "As a follow-up report to studies in previous decades, the present study covers 190 patients admitted to Roswell Park Memorial Institute for prostatic carcinoma during 1970 to 1974. The records of an additional 30 cases whose prostatic carcinoma was found incidentally at autopsy are included in this study for comparisons. The mean age at diagnosis was significantly lower than in the previous decade, with a greater proportion of progressions to an advanced clinical stage prior to admission to Roswell Park Memorial Institute. The incidence of cardiovascular deaths was much lower than in 1960 to 1969. Survival for all clinical stages was longer than that observed in the previous period and correlated with clinical stage. Serum acid and alkaline phosphatase levels progressively increased with clinical stage. Chemotherapy, which was rarely employed in the previous decade, found increasing usage during 1970 to 1974. There is a general trend to earlier diagnosis of prostatic adenocarcinoma.", "contents": "Prostatic cancer: evolution of treatment at a comprehensive center (1970-1974). As a follow-up report to studies in previous decades, the present study covers 190 patients admitted to Roswell Park Memorial Institute for prostatic carcinoma during 1970 to 1974. The records of an additional 30 cases whose prostatic carcinoma was found incidentally at autopsy are included in this study for comparisons. The mean age at diagnosis was significantly lower than in the previous decade, with a greater proportion of progressions to an advanced clinical stage prior to admission to Roswell Park Memorial Institute. The incidence of cardiovascular deaths was much lower than in 1960 to 1969. Survival for all clinical stages was longer than that observed in the previous period and correlated with clinical stage. Serum acid and alkaline phosphatase levels progressively increased with clinical stage. Chemotherapy, which was rarely employed in the previous decade, found increasing usage during 1970 to 1974. There is a general trend to earlier diagnosis of prostatic adenocarcinoma.", "PMID": 973289}
{"id": "pubmed23n0032_9226", "title": "Postprostatectomy incontinence: George Washington University technique.", "content": "The original concept of external compression on the urethra to provide urinary continence in the male has undergone significant modifications since the original work of Berry fifteen years ago. The technique used at George Washington University is a successful extension of Berry's concept. This article presents a very precise description of the operation. There has been only one failure in 13 patients referred to us, therefore the technique is recommended as an excellent means of correcting postprostatectomy incontinence.", "contents": "Postprostatectomy incontinence: George Washington University technique. The original concept of external compression on the urethra to provide urinary continence in the male has undergone significant modifications since the original work of Berry fifteen years ago. The technique used at George Washington University is a successful extension of Berry's concept. This article presents a very precise description of the operation. There has been only one failure in 13 patients referred to us, therefore the technique is recommended as an excellent means of correcting postprostatectomy incontinence.", "PMID": 973290}
{"id": "pubmed23n0032_9227", "title": "Factors related to host recognition of bacterial antigens which cause urinary infection.", "content": "Some gram-negative bacteria isolated from the urinary tract have on their surfaces antigens identical to or similar to those of human blood groups A, B, or (H) O. Human isoantibodies to these antigens appear to play some part in the immunologic response to urinary infections. It is postulated from this observation that human isoantibodies A and B persist because they are important segments in resistance to infections rather than reactions to other human tissues (as in transfusions or transplantation). To further assess the relationship between bacterial antigens similar to those of blood groups A or B and host isoantibody response we performed an in-depth study of this relationship in 13 patients with urinary infection of upper or lower tracts. Isoantibody of the immunoglobulin M class appeared to be the antibody which reacted with both bacteria and human erythrocytes. Patients with infections of the upper tracts had proportionately more cross-reacting anti-B antibody than those with lower tract infections, but the amounts of anti-A antibody were roughly equivalent in both types of infection. Response of a given host was genetically predetermined. Some deficiency in host recognition of bacterial antigens was noted since only 4 of 13 patients recognized bacterial A or B antigens while all normals did so. Study of the defined crossreactions between bacterial and blood group antigens offers further opportunities for definition of the genetic components of immunity to urinary infections.", "contents": "Factors related to host recognition of bacterial antigens which cause urinary infection. Some gram-negative bacteria isolated from the urinary tract have on their surfaces antigens identical to or similar to those of human blood groups A, B, or (H) O. Human isoantibodies to these antigens appear to play some part in the immunologic response to urinary infections. It is postulated from this observation that human isoantibodies A and B persist because they are important segments in resistance to infections rather than reactions to other human tissues (as in transfusions or transplantation). To further assess the relationship between bacterial antigens similar to those of blood groups A or B and host isoantibody response we performed an in-depth study of this relationship in 13 patients with urinary infection of upper or lower tracts. Isoantibody of the immunoglobulin M class appeared to be the antibody which reacted with both bacteria and human erythrocytes. Patients with infections of the upper tracts had proportionately more cross-reacting anti-B antibody than those with lower tract infections, but the amounts of anti-A antibody were roughly equivalent in both types of infection. Response of a given host was genetically predetermined. Some deficiency in host recognition of bacterial antigens was noted since only 4 of 13 patients recognized bacterial A or B antigens while all normals did so. Study of the defined crossreactions between bacterial and blood group antigens offers further opportunities for definition of the genetic components of immunity to urinary infections.", "PMID": 973291}
{"id": "pubmed23n0032_9228", "title": "Unilateral medullary sponge kidney: cause of persistent bacteriuria.", "content": "A patient with twenty years of persistent bacteriuria secondary to unilateral medullary sponge kidney, and in whom no antibiotic would sterilize the urine, was cured of her infection by nephrectomy in the presence of contralateral hydronephrosis. A review of the literature shows 23 cases of unilateral medullary sponge kidney in which nephrectomy or partial nephrectomy was apparently equally successful, although no bacteriologic follow-up data are given.", "contents": "Unilateral medullary sponge kidney: cause of persistent bacteriuria. A patient with twenty years of persistent bacteriuria secondary to unilateral medullary sponge kidney, and in whom no antibiotic would sterilize the urine, was cured of her infection by nephrectomy in the presence of contralateral hydronephrosis. A review of the literature shows 23 cases of unilateral medullary sponge kidney in which nephrectomy or partial nephrectomy was apparently equally successful, although no bacteriologic follow-up data are given.", "PMID": 973292}
{"id": "pubmed23n0032_9229", "title": "Blunt trauma to renal artery.", "content": "A case of intimal laceration of the renal artery by blunt trauma is presented. Although complete and prolonged functional loss was documented, total recovery was eventually observed. Postulates regarding pathogenesis are provided. The usefulness of arteriography for therapeutic and prognostic reasons is emphasized.", "contents": "Blunt trauma to renal artery. A case of intimal laceration of the renal artery by blunt trauma is presented. Although complete and prolonged functional loss was documented, total recovery was eventually observed. Postulates regarding pathogenesis are provided. The usefulness of arteriography for therapeutic and prognostic reasons is emphasized.", "PMID": 973293}
{"id": "pubmed23n0032_9230", "title": "Seven-year survival of child with rhabdomyosarcoma of prostate.", "content": "A case is reported of a twenty-two-month-old child who has survived for seven years without recurrence after multimodal therapy of a rhabdomyosarcoma of the prostate. The patient was treated with preoperative irradiation, radical surgery, and intraoperative and postoperative actinomycin D.", "contents": "Seven-year survival of child with rhabdomyosarcoma of prostate. A case is reported of a twenty-two-month-old child who has survived for seven years without recurrence after multimodal therapy of a rhabdomyosarcoma of the prostate. The patient was treated with preoperative irradiation, radical surgery, and intraoperative and postoperative actinomycin D.", "PMID": 973294}
{"id": "pubmed23n0032_9231", "title": "Cure of idiopathic priapism: new procedure for creating fistula between glans penis and corpora cavernosa.", "content": "A patient with idiopathic priapism of four days' duration had detumescence achieved in his hospital room using local anesthesia by creation of fistula between the glans penis and each corpora cavernosa bodies by means of a Travenol biopsy needle.", "contents": "Cure of idiopathic priapism: new procedure for creating fistula between glans penis and corpora cavernosa. A patient with idiopathic priapism of four days' duration had detumescence achieved in his hospital room using local anesthesia by creation of fistula between the glans penis and each corpora cavernosa bodies by means of a Travenol biopsy needle.", "PMID": 973296}
{"id": "pubmed23n0032_9232", "title": "Non functioning interstitial cell carcinoma of testis.", "content": "The authors add an additional case to the 13 cases of malignant interstitial cell tumor of the testis previously reported in the literature. It has been thought that the hallmark of metastatic interstitial cell tumor of the testis is endocrinopathy secondary to production of hormones by the tumor. In the case reported here there were no clinical or chemical manifestations of any endocrinopathy, making this case unique.", "contents": "Non functioning interstitial cell carcinoma of testis. The authors add an additional case to the 13 cases of malignant interstitial cell tumor of the testis previously reported in the literature. It has been thought that the hallmark of metastatic interstitial cell tumor of the testis is endocrinopathy secondary to production of hormones by the tumor. In the case reported here there were no clinical or chemical manifestations of any endocrinopathy, making this case unique.", "PMID": 973297}
{"id": "pubmed23n0032_9233", "title": "Epidermal cyst of median raphe.", "content": "Cysts of the penis are rare and references to them in standard textbooks are sketchy. A case report of a congenital epidermal cyst of the median raphe of the penis is presented; therapy involved excision of the mass. Review of the literature is given.", "contents": "Epidermal cyst of median raphe. Cysts of the penis are rare and references to them in standard textbooks are sketchy. A case report of a congenital epidermal cyst of the median raphe of the penis is presented; therapy involved excision of the mass. Review of the literature is given.", "PMID": 973298}
{"id": "pubmed23n0032_9234", "title": "Spontaneous rupture of renal pelvis.", "content": "A seventy-seven-year-old female with progressive abdominal sighs for twenty-four hours was found to have had a spontaneous rupture of the renal pelvis with massive urinary extravasation.", "contents": "Spontaneous rupture of renal pelvis. A seventy-seven-year-old female with progressive abdominal sighs for twenty-four hours was found to have had a spontaneous rupture of the renal pelvis with massive urinary extravasation.", "PMID": 973300}
{"id": "pubmed23n0032_9235", "title": "Uroradiology of diabetes mellitus.", "content": "Diabetes mellitus is a prevalent disorder, well controlled in many persons with prolongation of life. Several radiologic manifestations are sufficiently specific to suggest a diagnosis in the unidentified patient, but even more important is an awareness of the sometimes life-threatening complications of diabetes which can be diagnosed from uroradiologic studies. We review the following urinary tract manifestations and complications of diabetes: pyelonephritis, perinephric abscess, renal papillary necrosis, emphysematous pyelonephritis, emphysematous cystitis, fungus infections, calcification of the vas deferens, seminal vesicle, and intrarenal branches of the renal artery, neuropathic bladder, and renal failure.", "contents": "Uroradiology of diabetes mellitus. Diabetes mellitus is a prevalent disorder, well controlled in many persons with prolongation of life. Several radiologic manifestations are sufficiently specific to suggest a diagnosis in the unidentified patient, but even more important is an awareness of the sometimes life-threatening complications of diabetes which can be diagnosed from uroradiologic studies. We review the following urinary tract manifestations and complications of diabetes: pyelonephritis, perinephric abscess, renal papillary necrosis, emphysematous pyelonephritis, emphysematous cystitis, fungus infections, calcification of the vas deferens, seminal vesicle, and intrarenal branches of the renal artery, neuropathic bladder, and renal failure.", "PMID": 973301}
{"id": "pubmed23n0032_9236", "title": "[Significance of the type of trauma for the outcome of nerve injury].", "content": "The experience with 1896 restorative operations on injured nerve trunks shows a necessity to consider the problems of diagnosis, prognosis and choice of the treatment, and especially the results of the nerve suture, not in all patients with nerve injury but only in separate groups being comparable with respect to the kind and severity of the trauma. The probability of spontaneous recovery after cut and prick wounds is very small, if the signs of complete interruption of the conductivity are revealed from the very beginning. As a rule, nerve suture seems to be indicated after establishing the precise diagnosis. Rush and inadequate operations in primary surgical treatment of the wound, in particular, can cause an additional and severe trauma of the nerve, which repairing proves to be difficult and sometimes even impossible. In bone fractures the operation on injured nerves may be indicated after establishing the precise diagnosis during the period of \"justified wating\", taking into account the injury level and details of the clinical course. In traction lesions of the brachial plexus the correct diagnosis of the level and severity of the trauma must be established without an operation.", "contents": "[Significance of the type of trauma for the outcome of nerve injury]. The experience with 1896 restorative operations on injured nerve trunks shows a necessity to consider the problems of diagnosis, prognosis and choice of the treatment, and especially the results of the nerve suture, not in all patients with nerve injury but only in separate groups being comparable with respect to the kind and severity of the trauma. The probability of spontaneous recovery after cut and prick wounds is very small, if the signs of complete interruption of the conductivity are revealed from the very beginning. As a rule, nerve suture seems to be indicated after establishing the precise diagnosis. Rush and inadequate operations in primary surgical treatment of the wound, in particular, can cause an additional and severe trauma of the nerve, which repairing proves to be difficult and sometimes even impossible. In bone fractures the operation on injured nerves may be indicated after establishing the precise diagnosis during the period of \"justified wating\", taking into account the injury level and details of the clinical course. In traction lesions of the brachial plexus the correct diagnosis of the level and severity of the trauma must be established without an operation.", "PMID": 973308}
{"id": "pubmed23n0032_9237", "title": "[Indications for and technic of papillosphincterotomy].", "content": "The authors' experience with 45 papillosphincterotomies, performed for diseases of bile passages and chronic pancreatitis, is reported. The details of the operative technic, employed by the authors, are described. The technic of papillosphincterotomy on a conus-shape sound by means of a \"laser scalpel\" proved to be the most perfect one. Careful observation of the surgical technic made it possible to gain favourable results--only 1 of 45 operated patients died. Papillosphincterotomy is largely advocated in stenoses and concretions in the large duodenal papilla, strictures of choledocho-duodenoanastomoses, care should be taken not to overwiden indications to the operation concerned.", "contents": "[Indications for and technic of papillosphincterotomy]. The authors' experience with 45 papillosphincterotomies, performed for diseases of bile passages and chronic pancreatitis, is reported. The details of the operative technic, employed by the authors, are described. The technic of papillosphincterotomy on a conus-shape sound by means of a \"laser scalpel\" proved to be the most perfect one. Careful observation of the surgical technic made it possible to gain favourable results--only 1 of 45 operated patients died. Papillosphincterotomy is largely advocated in stenoses and concretions in the large duodenal papilla, strictures of choledocho-duodenoanastomoses, care should be taken not to overwiden indications to the operation concerned.", "PMID": 973315}
{"id": "pubmed23n0032_9238", "title": "[Secondary hemorrhage following trauma to the bones and soft tissues of the extremities].", "content": "An analysis of 16 cases of secondary hemorrhages in injuries of bones and soft tissues of the extremities is presented. The secondary hemorrhages occurred within the period from 16 hours to 27 days after the trauma. Angiographic study is a valuable adjunct for establishing the correct diagnosis. Arrosion of large arterial trunks was the adjunct for establishing the correct diagnosis. Arrosion of large arterial trunks was the basic cause of the secondary hemorrhage. In the prophylaxis of the latter a special importance is attached to a wide wound dissection in primary surgical treatment and to secure hemostasis, especially in the body portions having multilayer musculature.", "contents": "[Secondary hemorrhage following trauma to the bones and soft tissues of the extremities]. An analysis of 16 cases of secondary hemorrhages in injuries of bones and soft tissues of the extremities is presented. The secondary hemorrhages occurred within the period from 16 hours to 27 days after the trauma. Angiographic study is a valuable adjunct for establishing the correct diagnosis. Arrosion of large arterial trunks was the adjunct for establishing the correct diagnosis. Arrosion of large arterial trunks was the basic cause of the secondary hemorrhage. In the prophylaxis of the latter a special importance is attached to a wide wound dissection in primary surgical treatment and to secure hemostasis, especially in the body portions having multilayer musculature.", "PMID": 973324}
{"id": "pubmed23n0032_9239", "title": "[Fractures of the dens of the 2d cervical vertebra and their treatment].", "content": "Fractures of the dens axis make 10-15 per cent to all injuries of the cervical portion of the vertebral column. The potential danger of these injuries and difficulties of their treatment are conditioned by anatomo-functional features of this portion of the spine. The mechanism of fractures of the dens axis is not clear yet. Clinical manifestations of dens axis fractures are determined by the character and extent of its desplacement. Its diagnosis is somewhat difficult sometimes. Conservative therapy is not always a success. The existing methods of surgical treatment of dens axis fractures make it possible to gain the cure nearly in all cases.", "contents": "[Fractures of the dens of the 2d cervical vertebra and their treatment]. Fractures of the dens axis make 10-15 per cent to all injuries of the cervical portion of the vertebral column. The potential danger of these injuries and difficulties of their treatment are conditioned by anatomo-functional features of this portion of the spine. The mechanism of fractures of the dens axis is not clear yet. Clinical manifestations of dens axis fractures are determined by the character and extent of its desplacement. Its diagnosis is somewhat difficult sometimes. Conservative therapy is not always a success. The existing methods of surgical treatment of dens axis fractures make it possible to gain the cure nearly in all cases.", "PMID": 973325}
{"id": "pubmed23n0032_9240", "title": "[Treatment of intestinal paresis in children].", "content": "In the paper, a clinical experience with treatment of intestinal paresis in 545 children, aged from 1 day to 13 years, including 225 children operated upon for peritonitis is summarized. The authors differentiate 3 stages in the development of intestinal paresis depending on the degree of intensity of systemic and local disturbances. The employed methods of treatment in intestinal paresis were classified by the principle of their effect as 3 groups. Application of some or other method of the paresis therapy is determined by the stage of its development. In treatment of postoperative intestinal paresis a continuous peridural blockade is considered to be the method of choice. Utilization of the latter enabled the authors to reveal a number of postoperative complications: mechanic intestinal obstruction, incompetent anastomosis, etc.", "contents": "[Treatment of intestinal paresis in children]. In the paper, a clinical experience with treatment of intestinal paresis in 545 children, aged from 1 day to 13 years, including 225 children operated upon for peritonitis is summarized. The authors differentiate 3 stages in the development of intestinal paresis depending on the degree of intensity of systemic and local disturbances. The employed methods of treatment in intestinal paresis were classified by the principle of their effect as 3 groups. Application of some or other method of the paresis therapy is determined by the stage of its development. In treatment of postoperative intestinal paresis a continuous peridural blockade is considered to be the method of choice. Utilization of the latter enabled the authors to reveal a number of postoperative complications: mechanic intestinal obstruction, incompetent anastomosis, etc.", "PMID": 973330}
{"id": "pubmed23n0032_9241", "title": "[Recurrence of peptic ulcer].", "content": "In the paper, the causes, clinic and treatment in 15 patients with recurrent peptic ulcer after repeated operative procedures for duodenal ulcer are described. The main causes of the recurrence were as follows: the remaining of the mucous membrane of the antral gastric portion in Finsterer gastric resection for ulcer exclusion (5) and Zollinger-Ellison syndrome (10). The surgical therapy consisted in resection of the antral portion and repeated gastric resection (the first group) or in subtotal gastrectomy associated with trunk vagotomy (the second group).", "contents": "[Recurrence of peptic ulcer]. In the paper, the causes, clinic and treatment in 15 patients with recurrent peptic ulcer after repeated operative procedures for duodenal ulcer are described. The main causes of the recurrence were as follows: the remaining of the mucous membrane of the antral gastric portion in Finsterer gastric resection for ulcer exclusion (5) and Zollinger-Ellison syndrome (10). The surgical therapy consisted in resection of the antral portion and repeated gastric resection (the first group) or in subtotal gastrectomy associated with trunk vagotomy (the second group).", "PMID": 973334}
{"id": "pubmed23n0032_9242", "title": "Virological study of some influenza outbreaks in the period January--March 1975.", "content": "The virological study of some influenza outbreaks recorded in Romania in the period Jnauary--March 1975 resulted in the isolation of 29 influenza A2 virus strains antigenically close to the strains A2/Port Chalmers 1/73 and A2/England 42/72. The non-homogeneous antigenic structure of the isolates suggests the possible formation of natural hybrids.", "contents": "Virological study of some influenza outbreaks in the period January--March 1975. The virological study of some influenza outbreaks recorded in Romania in the period Jnauary--March 1975 resulted in the isolation of 29 influenza A2 virus strains antigenically close to the strains A2/Port Chalmers 1/73 and A2/England 42/72. The non-homogeneous antigenic structure of the isolates suggests the possible formation of natural hybrids.", "PMID": 973341}
{"id": "pubmed23n0032_9243", "title": "Distribution of hepatitis B virus subtypes in Romania.", "content": "The higher incidence of HB8Ag/ayw (71%) in sera collected from acute viral hepatitis cases, as well as from patients with chronic active hepatitis, healthy carriers and blood donors of 20 Romanian counties emphasizes the local prevalence of the ayw determinant in our country.", "contents": "Distribution of hepatitis B virus subtypes in Romania. The higher incidence of HB8Ag/ayw (71%) in sera collected from acute viral hepatitis cases, as well as from patients with chronic active hepatitis, healthy carriers and blood donors of 20 Romanian counties emphasizes the local prevalence of the ayw determinant in our country.", "PMID": 973342}
{"id": "pubmed23n0032_9244", "title": "Treatment and prevention of acute respiratory virus infections in children with leukocytic interferon.", "content": "Children with respiratory virus infections and healthy contacts were given leukocytic interferon (LIF) as treatment and prophylaxis. The results emphasize the effectiveness of the treatment when LIF was given on the 1st or 2nd day after onset of illness. In these cases the course was mild, of short duration and without complications. In children who did not receive LIF, the clinical course was more severe, the disease lasted longer, and complications were not uncommon. Prophylactic instillation of LIF succeeded in preventing the disease in 85.2% of the contacts; those who contracted the infection had a mild disease with insignificant complications. No allergic or intoxication phenomena were observed in children receiving LIF.", "contents": "Treatment and prevention of acute respiratory virus infections in children with leukocytic interferon. Children with respiratory virus infections and healthy contacts were given leukocytic interferon (LIF) as treatment and prophylaxis. The results emphasize the effectiveness of the treatment when LIF was given on the 1st or 2nd day after onset of illness. In these cases the course was mild, of short duration and without complications. In children who did not receive LIF, the clinical course was more severe, the disease lasted longer, and complications were not uncommon. Prophylactic instillation of LIF succeeded in preventing the disease in 85.2% of the contacts; those who contracted the infection had a mild disease with insignificant complications. No allergic or intoxication phenomena were observed in children receiving LIF.", "PMID": 973346}
{"id": "pubmed23n0032_9245", "title": "Comparative study of cutaneous scars and HAI antibodies after smallpox revaccination.", "content": "The number and aspect of cutaneous scars and the HAI antibody titer were recorded in 154 20-21-year-old subjects before smallpox revaccination, 30 and 300 days afterwards. In 38.3% of the vaccinees there were no postvaccinal scars 300 days after revaccination, but the absence of residual scars did not necessarily indicate a failure of immunization, since the increase in mean HAI antibody titer was significant in most of the cases.", "contents": "Comparative study of cutaneous scars and HAI antibodies after smallpox revaccination. The number and aspect of cutaneous scars and the HAI antibody titer were recorded in 154 20-21-year-old subjects before smallpox revaccination, 30 and 300 days afterwards. In 38.3% of the vaccinees there were no postvaccinal scars 300 days after revaccination, but the absence of residual scars did not necessarily indicate a failure of immunization, since the increase in mean HAI antibody titer was significant in most of the cases.", "PMID": 973343}
{"id": "pubmed23n0032_9246", "title": "Investigations on the distribution of HBs antigen subtypes.", "content": "The study was performed in 2,054 sera collected from HBsAg-positive acute viral hepatitis (AVH) patients within the first days after onset. HBsAg were subtyped by immunodiffusion (it was possible to specify the antigenic subdeterminant in 1,816 sera) and titrated by counterelectrophoresis. The results were analysed according to the territory and to the patients' sex and age. The overall ad:ay ratio was of 18:82 and zonal differences in subtype ratio were found. A marked predominance of male subjects (57%) among HBsAg-positive AVH patients was observed, especially in younger age groups. Half of the cases could be included in the age groups from 5 to 29 years. Higher ad:ay values were found at low antigen titers.", "contents": "Investigations on the distribution of HBs antigen subtypes. The study was performed in 2,054 sera collected from HBsAg-positive acute viral hepatitis (AVH) patients within the first days after onset. HBsAg were subtyped by immunodiffusion (it was possible to specify the antigenic subdeterminant in 1,816 sera) and titrated by counterelectrophoresis. The results were analysed according to the territory and to the patients' sex and age. The overall ad:ay ratio was of 18:82 and zonal differences in subtype ratio were found. A marked predominance of male subjects (57%) among HBsAg-positive AVH patients was observed, especially in younger age groups. Half of the cases could be included in the age groups from 5 to 29 years. Higher ad:ay values were found at low antigen titers.", "PMID": 973347}
{"id": "pubmed23n0032_9247", "title": "[Traumatic cerebral thrombosis (author's transl)].", "content": "The observation of 6 patients with traumatic cerebral thrombosis following closed injury to the skull stimulated this compilation and review of the relevant literature on this subject. Thrombosis of the internal carotid artery and the vertebral artery head the list and do not only come about after blunt trauma to the neck, in which case traumatic aetiology is always thought of in conjunction with the appearance of appropriate neurological symptoms, but also arise from injuries to the skull. In the latter circumstances, the cerebral symptoms will usually be attributed to an epidural or subdural haematoma, particularly if there is a symptom-free period. This happens especially after minor injuries to the skull. In fact, traumatic thrombosis is seen less frequently than extracerebral haematomas, but is nevertheless repeatedly encountered owing to the great number of traffic accidents. Definitive diagnosis can be made only by angiography of the cerebral vessels, above all of the internal carotid artery. Similar to the prognosis of any disease caused by vascular occlusion, the prognosis as to the extent of recovery from the cerebral focal symptoms depends on the sufficiency of the collateral circulation, whereby surgical removal of the thrombus at the earliest possible moment appears to absolutely indicated.", "contents": "[Traumatic cerebral thrombosis (author's transl)]. The observation of 6 patients with traumatic cerebral thrombosis following closed injury to the skull stimulated this compilation and review of the relevant literature on this subject. Thrombosis of the internal carotid artery and the vertebral artery head the list and do not only come about after blunt trauma to the neck, in which case traumatic aetiology is always thought of in conjunction with the appearance of appropriate neurological symptoms, but also arise from injuries to the skull. In the latter circumstances, the cerebral symptoms will usually be attributed to an epidural or subdural haematoma, particularly if there is a symptom-free period. This happens especially after minor injuries to the skull. In fact, traumatic thrombosis is seen less frequently than extracerebral haematomas, but is nevertheless repeatedly encountered owing to the great number of traffic accidents. Definitive diagnosis can be made only by angiography of the cerebral vessels, above all of the internal carotid artery. Similar to the prognosis of any disease caused by vascular occlusion, the prognosis as to the extent of recovery from the cerebral focal symptoms depends on the sufficiency of the collateral circulation, whereby surgical removal of the thrombus at the earliest possible moment appears to absolutely indicated.", "PMID": 973373}
{"id": "pubmed23n0032_9248", "title": "[Thrombosis of the cerebral vessels following closed cranial trauma (author's transl)].", "content": "The case histories are presented of 6 patients, 5 of whom developed carotid artery thrombosis (the left vessel being affected in 4 cases and the right only in 1 case) following closed cranio-cerebral trauma; the 6th patient developed thrombosis of the posterior cerebral artery, as confirmed at autopsy. The factors involved in the aetiology of these events are discussed on the basis of the case reports.", "contents": "[Thrombosis of the cerebral vessels following closed cranial trauma (author's transl)]. The case histories are presented of 6 patients, 5 of whom developed carotid artery thrombosis (the left vessel being affected in 4 cases and the right only in 1 case) following closed cranio-cerebral trauma; the 6th patient developed thrombosis of the posterior cerebral artery, as confirmed at autopsy. The factors involved in the aetiology of these events are discussed on the basis of the case reports.", "PMID": 973374}
{"id": "pubmed23n0032_9249", "title": "[Angiographic disappearance of fibromuscular dysplasia of the carotid artery in childhood (author's transl)].", "content": "Fibromuscular dysplasia (FMD) of the left carotid artery was diagnosed on the basis of the typical angiographic changes in a seven-year-old girl suffering from acute hemiplegia. A repeat angiography of both carotid and renal arteries 8 months later revealed no pathological changes. The diagnosis of FMD of the carotid arteries is extemely difficult and requires a biopsy or repeat angiography for verification.", "contents": "[Angiographic disappearance of fibromuscular dysplasia of the carotid artery in childhood (author's transl)]. Fibromuscular dysplasia (FMD) of the left carotid artery was diagnosed on the basis of the typical angiographic changes in a seven-year-old girl suffering from acute hemiplegia. A repeat angiography of both carotid and renal arteries 8 months later revealed no pathological changes. The diagnosis of FMD of the carotid arteries is extemely difficult and requires a biopsy or repeat angiography for verification.", "PMID": 973375}
{"id": "pubmed23n0032_9250", "title": "[Finger Microtremor--an experimental study (author's transl)].", "content": "This paper deals with the recording of finger microtremor using a self-constructed apparatus (amplitudes 0.5 to 0.05 mm). 32 healthy subjects aged 7 to 60 years were examined by means of a test set permitting the exact recording of the amplitudes and frequencies of the right middle finger movement. Special attention was given to the vertical plane of the tremor in order to permit more exact demonstration. Frequencies of 5 to 12 cps. were recorded, the mean frequency being 7.55+/-1.12 cps. The minimum amplitude was 0.02 and the maximum recorded amplitude amounted to 1.4 mm, with a mean amplitude of 0.12+/- 0.07 mm. No significant differences in amplitude or frequency were noted with respect to age or sex. This method is useful in the accurate quantitative assessment of every kind of tremor.", "contents": "[Finger Microtremor--an experimental study (author's transl)]. This paper deals with the recording of finger microtremor using a self-constructed apparatus (amplitudes 0.5 to 0.05 mm). 32 healthy subjects aged 7 to 60 years were examined by means of a test set permitting the exact recording of the amplitudes and frequencies of the right middle finger movement. Special attention was given to the vertical plane of the tremor in order to permit more exact demonstration. Frequencies of 5 to 12 cps. were recorded, the mean frequency being 7.55+/-1.12 cps. The minimum amplitude was 0.02 and the maximum recorded amplitude amounted to 1.4 mm, with a mean amplitude of 0.12+/- 0.07 mm. No significant differences in amplitude or frequency were noted with respect to age or sex. This method is useful in the accurate quantitative assessment of every kind of tremor.", "PMID": 973376}
{"id": "pubmed23n0032_9251", "title": "[Effect of Ouabain on cerebral blood flow (author's transl)].", "content": "The effect of 0.25 mg ouabain on cerebral blood flow (CBF) was investigated in patients with and without cerebrovascular disease using the xenon clearance method. The 36 patients included in this study did not show any signs of heart failure. Ouabain increased the CBF and this effect was demonstrable 15, as well as 90 min. after administration. This effect was proven statistically using the t-test for a comparison of the values with spontaneous changes in a control group without medication. The perfusion of pathologically-supplied brain regions was altered in the same way as the hemispheric flow; changes in the distribution of blood in the way of a steal effect were not observed. The haemodynamic parameters do not indicate a primary cardiac effect. Hence, an influence of ouabain on cerebral vessels might be responsible. The present results support reported clinical experience with ouabain for the treatment of patients with cerebrosvascular disease.", "contents": "[Effect of Ouabain on cerebral blood flow (author's transl)]. The effect of 0.25 mg ouabain on cerebral blood flow (CBF) was investigated in patients with and without cerebrovascular disease using the xenon clearance method. The 36 patients included in this study did not show any signs of heart failure. Ouabain increased the CBF and this effect was demonstrable 15, as well as 90 min. after administration. This effect was proven statistically using the t-test for a comparison of the values with spontaneous changes in a control group without medication. The perfusion of pathologically-supplied brain regions was altered in the same way as the hemispheric flow; changes in the distribution of blood in the way of a steal effect were not observed. The haemodynamic parameters do not indicate a primary cardiac effect. Hence, an influence of ouabain on cerebral vessels might be responsible. The present results support reported clinical experience with ouabain for the treatment of patients with cerebrosvascular disease.", "PMID": 973377}
{"id": "pubmed23n0032_9252", "title": "[Severe intoxication with leponex (author's transl)].", "content": "The clinical picture and the therapeutic management of a severe case of self-poisoning with Leponex (clozapin) are presented. In particular, the grave complications, their possible aetiology and the value of the administration of cortisone are discussed. Forced diuresis or haemodialysis are ineffective measures on account of the low serum concentrations of Leponex.", "contents": "[Severe intoxication with leponex (author's transl)]. The clinical picture and the therapeutic management of a severe case of self-poisoning with Leponex (clozapin) are presented. In particular, the grave complications, their possible aetiology and the value of the administration of cortisone are discussed. Forced diuresis or haemodialysis are ineffective measures on account of the low serum concentrations of Leponex.", "PMID": 973378}
{"id": "pubmed23n0032_9253", "title": "[Use of ultrasound methods in vascular and circulatory diseases (author's transl)].", "content": "The paper summarizes the principles, techniques and efficiency of ultrasound methods as indicator systems in the assessment of the peripheral and cerebral arteries; our studies predominantly refer to the use of these simple, non-invasive procedures in combination with sphygmo-oscillography and dynamographic circulation analysis, respectively. Ultrasound Doppler flowmetry (Pulse Doppler method), which permits relative measurements of the flow velocity in the peripheral haemodynamic regions, is of great importance in the rapid ascertainment of the patency of the major arterial trunks and the non-palpable vessels and also in the diagnosis, localization and follow-up control of stenoses and occlusions. Changes in the flow velocity due to organic or functional factors can be recorded by this method acoustically, oscilloscopically and graphically; measurements of the systolic blood pressure in the limb arteries by the Doppler technique provide semi-quantitative information in regard to the condition of the circulation. The registration of Doppler signals over the supra-orbital artery before and after compression of the superficial temporal artery and the common carotid artery has become established as a screening procedure for stenoses of the internal carotid artery. Ultrasound echography, which enables pulse registration and determination of vessel diameters, especially of the carotid siphon, can also be used for the detection of obstructions in the region of the internal carotid artery, as well as the vertebral artery.", "contents": "[Use of ultrasound methods in vascular and circulatory diseases (author's transl)]. The paper summarizes the principles, techniques and efficiency of ultrasound methods as indicator systems in the assessment of the peripheral and cerebral arteries; our studies predominantly refer to the use of these simple, non-invasive procedures in combination with sphygmo-oscillography and dynamographic circulation analysis, respectively. Ultrasound Doppler flowmetry (Pulse Doppler method), which permits relative measurements of the flow velocity in the peripheral haemodynamic regions, is of great importance in the rapid ascertainment of the patency of the major arterial trunks and the non-palpable vessels and also in the diagnosis, localization and follow-up control of stenoses and occlusions. Changes in the flow velocity due to organic or functional factors can be recorded by this method acoustically, oscilloscopically and graphically; measurements of the systolic blood pressure in the limb arteries by the Doppler technique provide semi-quantitative information in regard to the condition of the circulation. The registration of Doppler signals over the supra-orbital artery before and after compression of the superficial temporal artery and the common carotid artery has become established as a screening procedure for stenoses of the internal carotid artery. Ultrasound echography, which enables pulse registration and determination of vessel diameters, especially of the carotid siphon, can also be used for the detection of obstructions in the region of the internal carotid artery, as well as the vertebral artery.", "PMID": 973379}
{"id": "pubmed23n0032_9254", "title": "[Blood pressure analysis by means of an ultrasonic blood pressure monitor during the course of rehabilitative treatment (author's transl)].", "content": "A statistic evaluation of the daily blood pressure values at rest during a 4-week period of active training combined with physiotherapy and medical baths indicated a decrease of 20 mm Bg in systolic pressure and of 9 mm Hg in diastolic pressure in patients with hypertension of various grades. The resting blood pressure shows a tendency to become normalized during the course of this therapy with phases of maximum negative decline. However, a standardized simple submaximla ergometric load test carried out weekly demonstrated that there were only minimal changes in systolic and diastolic blood pressure during exercise. In comparison with observations made in other spas, these results demonstrate a uniform reaction of the human organism to this particular form of irritative therapy independent of the particular features of the spas and the special constituents of the waters.", "contents": "[Blood pressure analysis by means of an ultrasonic blood pressure monitor during the course of rehabilitative treatment (author's transl)]. A statistic evaluation of the daily blood pressure values at rest during a 4-week period of active training combined with physiotherapy and medical baths indicated a decrease of 20 mm Bg in systolic pressure and of 9 mm Hg in diastolic pressure in patients with hypertension of various grades. The resting blood pressure shows a tendency to become normalized during the course of this therapy with phases of maximum negative decline. However, a standardized simple submaximla ergometric load test carried out weekly demonstrated that there were only minimal changes in systolic and diastolic blood pressure during exercise. In comparison with observations made in other spas, these results demonstrate a uniform reaction of the human organism to this particular form of irritative therapy independent of the particular features of the spas and the special constituents of the waters.", "PMID": 973380}
{"id": "pubmed23n0032_9255", "title": "[A new antihypertensive imidazoline derivative (St 600, Fluoronidine): I. Haemodynamic investigations at rest and during exercise (author's transl)].", "content": "Treatment of 12 hypertensive patients with a new imidazoline derivative, St 600 (1.5 to 12.0 mg daily over at least 7 days) resulted in a significant lowering of the blood pressure, mostly to normal values, parallelled by a reduced heart heart rate. A significant decrease in arterial resistance was observed at rest and during exercise in 6 of the patients, whereas a slight increase or no change was recorded in the remaining 6 patients. The changes in resistance in the pulmonary circulation were analogous to those in the systematic circulation for the 2 groups, respectively. The latter half of the patients displayed a decrease in stroke volume, cardiac index and oxygen consumption. No negative inotropic effect was observed.", "contents": "[A new antihypertensive imidazoline derivative (St 600, Fluoronidine): I. Haemodynamic investigations at rest and during exercise (author's transl)]. Treatment of 12 hypertensive patients with a new imidazoline derivative, St 600 (1.5 to 12.0 mg daily over at least 7 days) resulted in a significant lowering of the blood pressure, mostly to normal values, parallelled by a reduced heart heart rate. A significant decrease in arterial resistance was observed at rest and during exercise in 6 of the patients, whereas a slight increase or no change was recorded in the remaining 6 patients. The changes in resistance in the pulmonary circulation were analogous to those in the systematic circulation for the 2 groups, respectively. The latter half of the patients displayed a decrease in stroke volume, cardiac index and oxygen consumption. No negative inotropic effect was observed.", "PMID": 973381}
{"id": "pubmed23n0032_9256", "title": "[The alcoholized pedestrian as victim of fatal traffic accidents (author's transl)].", "content": "The following results were obtained in an analysis of the road accidents which occurred in Vienna and its environs over the past ten years with a fatal outcome for the involved pedestrians: 39.3% of the pedestrains who died in an accident had a blood alcohol concentration of at least 0.8%, the average amounting to 2.15%. As many as 60.2% of the male victims were alcoholized, whereas only 7.6% of the women were alcoholized. The female victims were mostly elderly, 86% being older than 60 years, whereas many of the male victims were middle-aged. These middle-aged male pedestrians were frequently drunk; among the men aged between 40 and 49 the rate of alcoholization soared to 88%. The peak time of fatal traffic accidents involving alcoholized pedestrians lies in the hours before midnight. The number of pensioners among the sober victims is raised in comparison with the population rate, whilst an unexpectedly high percentage of unskilled workers found in the alcoholized group. In 7.6% of the alcoholized victime the autopsy revealed cirrhosis of the liver; 27.1% had fatty degeneration of the liver. Nearly as many female pedestrians met with a fatal road accident as males within the city boundaries. Accordingly, the percentage of alcoholized victims in the city (31.7%) is low compared with the rural areas, where about 60% of the victims had a blood alcohol concentration of at least 0.8%.", "contents": "[The alcoholized pedestrian as victim of fatal traffic accidents (author's transl)]. The following results were obtained in an analysis of the road accidents which occurred in Vienna and its environs over the past ten years with a fatal outcome for the involved pedestrians: 39.3% of the pedestrains who died in an accident had a blood alcohol concentration of at least 0.8%, the average amounting to 2.15%. As many as 60.2% of the male victims were alcoholized, whereas only 7.6% of the women were alcoholized. The female victims were mostly elderly, 86% being older than 60 years, whereas many of the male victims were middle-aged. These middle-aged male pedestrians were frequently drunk; among the men aged between 40 and 49 the rate of alcoholization soared to 88%. The peak time of fatal traffic accidents involving alcoholized pedestrians lies in the hours before midnight. The number of pensioners among the sober victims is raised in comparison with the population rate, whilst an unexpectedly high percentage of unskilled workers found in the alcoholized group. In 7.6% of the alcoholized victime the autopsy revealed cirrhosis of the liver; 27.1% had fatty degeneration of the liver. Nearly as many female pedestrians met with a fatal road accident as males within the city boundaries. Accordingly, the percentage of alcoholized victims in the city (31.7%) is low compared with the rural areas, where about 60% of the victims had a blood alcohol concentration of at least 0.8%.", "PMID": 973382}
{"id": "pubmed23n0032_9257", "title": "[Human urinary fluoride excretion at various ages (author's transl)].", "content": "3 groups, each consisting of 5 subjects in the following age ranges (A 4 to 6 years, B 25 to 45 years, C 60 to 70 years) were given fluoride in the form of a single dose of 6 mg. Fluoride excretion in the urine was subsequently investigated over a period of 24 hours. In a second experiment a daily dose of 6 mg of F- was given to the 3 groups over a period of 10 days. The urinary excretion was determined in 24-hour urine samples. The results are presented in two figures. 1. A time drift in urinary fluoride excretion in the direction of delayed fluoride metabolism was seen in group C subjects. 2. A periodic increase in the urinary fluoride values was also seen in these elderly subjects, indicative of an altered regulatory mechanism.", "contents": "[Human urinary fluoride excretion at various ages (author's transl)]. 3 groups, each consisting of 5 subjects in the following age ranges (A 4 to 6 years, B 25 to 45 years, C 60 to 70 years) were given fluoride in the form of a single dose of 6 mg. Fluoride excretion in the urine was subsequently investigated over a period of 24 hours. In a second experiment a daily dose of 6 mg of F- was given to the 3 groups over a period of 10 days. The urinary excretion was determined in 24-hour urine samples. The results are presented in two figures. 1. A time drift in urinary fluoride excretion in the direction of delayed fluoride metabolism was seen in group C subjects. 2. A periodic increase in the urinary fluoride values was also seen in these elderly subjects, indicative of an altered regulatory mechanism.", "PMID": 973383}
{"id": "pubmed23n0032_9258", "title": "[Fatal outcome of a case with calcifying alcoholic pancreatitis (author's transl)].", "content": "A case report is presented of a 38-year-old alcoholic welfare patient. Drainage of a pancreatic abscess, which had to be repeated, pyloromyotomy, cholecystectomy and sphincterotomy were undertaken in 1972 at another hospital. He was admitted on the present occasion because of weight loss, severe attacks of pain and diabetes. At operation multiple necrotic areas were found in the pancreas, with many stones in the parenchymatous tissue and in the main pancreatic duct and one large stone close to the pailla acting like a valve. Sub-total duodenopancreatectomy, resection of the pyloric region of the stomach, retrocolic hepaticojejunostomy and gastroenteroanastomosis was performed. The postoperative recovery took place without complications. 5 days after discharge the patient died in a hypoglycaemic coma at another hospital. He had administered 400 U. insulin to himself whilst in a drunken state. A short description is given of the aetiology and pathogenesis of calcifying pancreatitis. The choice of the surgical technique depends on the operative findings and the aim of therapy. Attention is called to the increase in late mortality in patients with pancreatectomy who do not abstain from alcohol.", "contents": "[Fatal outcome of a case with calcifying alcoholic pancreatitis (author's transl)]. A case report is presented of a 38-year-old alcoholic welfare patient. Drainage of a pancreatic abscess, which had to be repeated, pyloromyotomy, cholecystectomy and sphincterotomy were undertaken in 1972 at another hospital. He was admitted on the present occasion because of weight loss, severe attacks of pain and diabetes. At operation multiple necrotic areas were found in the pancreas, with many stones in the parenchymatous tissue and in the main pancreatic duct and one large stone close to the pailla acting like a valve. Sub-total duodenopancreatectomy, resection of the pyloric region of the stomach, retrocolic hepaticojejunostomy and gastroenteroanastomosis was performed. The postoperative recovery took place without complications. 5 days after discharge the patient died in a hypoglycaemic coma at another hospital. He had administered 400 U. insulin to himself whilst in a drunken state. A short description is given of the aetiology and pathogenesis of calcifying pancreatitis. The choice of the surgical technique depends on the operative findings and the aim of therapy. Attention is called to the increase in late mortality in patients with pancreatectomy who do not abstain from alcohol.", "PMID": 973384}
{"id": "pubmed23n0032_9259", "title": "[The diagnosis of IgD-Myelomatosis (author's transl)].", "content": "Two patients with IgD-lambda myelomatosis are presented and the differential diagnosis is discussed. Typical features of this disease are the high incidence of Bence-Jones proteinuria, osteolytic lesions, amyloidosis and the predominance of male patients. Furthermore, an augmentation of serum IgD level to 165 mg% was observed in a 22-year-old female patient with presumed Coxsackie myocarditis. The theories in regard to IgD function are discussed.", "contents": "[The diagnosis of IgD-Myelomatosis (author's transl)]. Two patients with IgD-lambda myelomatosis are presented and the differential diagnosis is discussed. Typical features of this disease are the high incidence of Bence-Jones proteinuria, osteolytic lesions, amyloidosis and the predominance of male patients. Furthermore, an augmentation of serum IgD level to 165 mg% was observed in a 22-year-old female patient with presumed Coxsackie myocarditis. The theories in regard to IgD function are discussed.", "PMID": 973385}
{"id": "pubmed23n0032_9260", "title": "[Blocking and binding antibodies to intrinsic factor and their behaviour during oral therapy with intrinsic factor-vitamin B 12 complex (author's transl)].", "content": "13 patients with pernicious anaemia and 2 patients who had been subjected to total gastrectomy were treated orally with a hog instrinsic factor preparation (IF) for 1 to 4.5 years. During this therapy 11 of the patients with pernicious anaemia and both gastrectomized patients developed blocking and binding antibodies to IF. Antibodies already present before the commencement of therapy showed an increase in titre. All patients remained in complete haematological remission. The conclusion is drawn that circulating antibodies to IF do not play any significant role in the absorption of the hog IF-B 12 complex.", "contents": "[Blocking and binding antibodies to intrinsic factor and their behaviour during oral therapy with intrinsic factor-vitamin B 12 complex (author's transl)]. 13 patients with pernicious anaemia and 2 patients who had been subjected to total gastrectomy were treated orally with a hog instrinsic factor preparation (IF) for 1 to 4.5 years. During this therapy 11 of the patients with pernicious anaemia and both gastrectomized patients developed blocking and binding antibodies to IF. Antibodies already present before the commencement of therapy showed an increase in titre. All patients remained in complete haematological remission. The conclusion is drawn that circulating antibodies to IF do not play any significant role in the absorption of the hog IF-B 12 complex.", "PMID": 973386}
{"id": "pubmed23n0032_9261", "title": "[A rapid direct latex agglutination test for HCG (author's transl)].", "content": "792 urine samples from pregnant patients were investigated by a direct latex agglutination test (LA). Results of this slide test were compared with data derived from a haemagglutination inhibition test (HI). The same results were obtained by both pregnancy tests in 768 (96.7%) out of 792 urine samples. The pregnancy test was negative in 20 cases (2.5%) as assessed by HI, whereas a positive result was recorded with the LA in these cases. Seven were cases of early pregnancy and control tests performed by HI became positive at a later date. The remaining 13 (1.6%) of these patients belonged to a group of pathological pregnancies (missed abortion, threatened abortion, incomplete abortion and ectopic pregnancy). The slide test is more sensitive (1000 I.U. HCG/1 urine) than the HI (1500 I.U.HCG/1 urine). No false positive results were obtained with the LA; false negative results were registered in only 0.5% of cases. A semi-quantitative HCG determination was performed by means of the tube and slide test in 29 urine samples. However, agreement of the data by the two methods was relatively poor, owing to the higher sensitivity of the LA, with consequent inaccurate assessment of HCG excretion. Not much importance need be attached to this finding in view of the diagnostic and prognostic deficiencies of HCG determination. The new slide test was found to be a rapid, simple and accurate pregnancy test.", "contents": "[A rapid direct latex agglutination test for HCG (author's transl)]. 792 urine samples from pregnant patients were investigated by a direct latex agglutination test (LA). Results of this slide test were compared with data derived from a haemagglutination inhibition test (HI). The same results were obtained by both pregnancy tests in 768 (96.7%) out of 792 urine samples. The pregnancy test was negative in 20 cases (2.5%) as assessed by HI, whereas a positive result was recorded with the LA in these cases. Seven were cases of early pregnancy and control tests performed by HI became positive at a later date. The remaining 13 (1.6%) of these patients belonged to a group of pathological pregnancies (missed abortion, threatened abortion, incomplete abortion and ectopic pregnancy). The slide test is more sensitive (1000 I.U. HCG/1 urine) than the HI (1500 I.U.HCG/1 urine). No false positive results were obtained with the LA; false negative results were registered in only 0.5% of cases. A semi-quantitative HCG determination was performed by means of the tube and slide test in 29 urine samples. However, agreement of the data by the two methods was relatively poor, owing to the higher sensitivity of the LA, with consequent inaccurate assessment of HCG excretion. Not much importance need be attached to this finding in view of the diagnostic and prognostic deficiencies of HCG determination. The new slide test was found to be a rapid, simple and accurate pregnancy test.", "PMID": 973387}
{"id": "pubmed23n0032_9262", "title": "[Treatment of interstitial pulmonary fibrosis with D-penicillamine in progressive scleroderma: a long-term study (author's transl)].", "content": "5 patients suffering from progressive scleroderma with interstitial pulmonary fibrosis have been treated since 1971 with D-penicillamine (Artamin) as basic therapeutic agent. All patients showed a significant tendency towards normalization of the pathological findings in regard to functional capacity of the lungs and scintigraphic and radiological assessment. These results, in our opinion, justify the recommendation of a new indication for D-penicillamine therapy, namely in cases of progressive scleroderma with interstitial pulmonary fibrosis.", "contents": "[Treatment of interstitial pulmonary fibrosis with D-penicillamine in progressive scleroderma: a long-term study (author's transl)]. 5 patients suffering from progressive scleroderma with interstitial pulmonary fibrosis have been treated since 1971 with D-penicillamine (Artamin) as basic therapeutic agent. All patients showed a significant tendency towards normalization of the pathological findings in regard to functional capacity of the lungs and scintigraphic and radiological assessment. These results, in our opinion, justify the recommendation of a new indication for D-penicillamine therapy, namely in cases of progressive scleroderma with interstitial pulmonary fibrosis.", "PMID": 973388}
{"id": "pubmed23n0032_9263", "title": "[Xeroderma pigmentosum with normal excision-repair capacity and decreased U-V tolerance (author's transl)].", "content": "Unscheduled DNA synthesis was investigated in the peripheral lymphocytes of a 31-year-old woman suffering from Xeroderma pigmentosum, clinically manifest since early childhood. The present data indicate a normal level of excision repair capacity, though U-V sensitivity was increased. The present case is considered to be a new variant of the syndrome Xeroderma pigmentosum, demonstrating once more its genetic heterogeneity. The question of a relationship between the observed data and the known occurrence of carcinoma in the light-exposed skin areas remains to be answered.", "contents": "[Xeroderma pigmentosum with normal excision-repair capacity and decreased U-V tolerance (author's transl)]. Unscheduled DNA synthesis was investigated in the peripheral lymphocytes of a 31-year-old woman suffering from Xeroderma pigmentosum, clinically manifest since early childhood. The present data indicate a normal level of excision repair capacity, though U-V sensitivity was increased. The present case is considered to be a new variant of the syndrome Xeroderma pigmentosum, demonstrating once more its genetic heterogeneity. The question of a relationship between the observed data and the known occurrence of carcinoma in the light-exposed skin areas remains to be answered.", "PMID": 973389}
{"id": "pubmed23n0032_9264", "title": "[The effect of vinblastine on DNA metabolism in turnover cells (author's transl)].", "content": "Studies on the effect of Vinblastine on normal and tumour cell show that semi-conservative DNA synthesis correlates with the enzymatic activity of thymidine kinase. DNA repair investigations performed with Yoshida-Ascites cells indicate an inhibitory effect on the ligase system after Vinblastine treatment and gamma irradiation.", "contents": "[The effect of vinblastine on DNA metabolism in turnover cells (author's transl)]. Studies on the effect of Vinblastine on normal and tumour cell show that semi-conservative DNA synthesis correlates with the enzymatic activity of thymidine kinase. DNA repair investigations performed with Yoshida-Ascites cells indicate an inhibitory effect on the ligase system after Vinblastine treatment and gamma irradiation.", "PMID": 973390}
{"id": "pubmed23n0032_9265", "title": "[Mosaic (Chimeric) animals: their experimental production and application (author's transl)].", "content": "A method introduced by Tarkowski permits the aggregation (mosaic-like fusion) of mammalian eggs in the early stages of division. In this paper, a modification of the Tarkowski method designed to create multiparental (mostly quadriparental) chimeric mice is discussed. The method described is simple, more practical and time-saving. The problems in analysing clonal processes of development in mammals and the mechanisms connected with sex differentiation and tumour development are discussed.", "contents": "[Mosaic (Chimeric) animals: their experimental production and application (author's transl)]. A method introduced by Tarkowski permits the aggregation (mosaic-like fusion) of mammalian eggs in the early stages of division. In this paper, a modification of the Tarkowski method designed to create multiparental (mostly quadriparental) chimeric mice is discussed. The method described is simple, more practical and time-saving. The problems in analysing clonal processes of development in mammals and the mechanisms connected with sex differentiation and tumour development are discussed.", "PMID": 973391}
{"id": "pubmed23n0032_9266", "title": "Glycemia and plasma IRI level after tolbutamide in hyposomatrophic dwarfs treated with HGH.", "content": "The insulinogenic function of the pancreatic islets was assessed by the use of tolbutamide stimulation in 25 children with hyposomatotrophic dwarfism (HSD). The authors suggest that the low insulin release and/or synthesis is responsible for slight growth response of these children to HGH treatment. There may also be a causative relation between this low insulin response and the tendency of children with HSD to develop diabetes after HGH treatment.", "contents": "Glycemia and plasma IRI level after tolbutamide in hyposomatrophic dwarfs treated with HGH. The insulinogenic function of the pancreatic islets was assessed by the use of tolbutamide stimulation in 25 children with hyposomatotrophic dwarfism (HSD). The authors suggest that the low insulin release and/or synthesis is responsible for slight growth response of these children to HGH treatment. There may also be a causative relation between this low insulin response and the tendency of children with HSD to develop diabetes after HGH treatment.", "PMID": 973430}
{"id": "pubmed23n0032_9267", "title": "Nuclear RNA in the mouse spleen at the peak of primary immune response.", "content": "Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.", "contents": "Nuclear RNA in the mouse spleen at the peak of primary immune response. Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.", "PMID": 973431}
{"id": "pubmed23n0032_9268", "title": "Cytoplasmic RNA in the mouse spleen at the peak of primary immune response.", "content": "Results of studies on the synthesis of different fractions of cytoplasmic RNA in the spleens of immunized mice at the peak of primary immune response are reported. RNA was extracted by the phenol-detergent procedure from the cytoplasm of spleen cells of mice immunized with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis and transport from nucleus to cytoplasm of 28S, 18S and 4S fractions of RNA.", "contents": "Cytoplasmic RNA in the mouse spleen at the peak of primary immune response. Results of studies on the synthesis of different fractions of cytoplasmic RNA in the spleens of immunized mice at the peak of primary immune response are reported. RNA was extracted by the phenol-detergent procedure from the cytoplasm of spleen cells of mice immunized with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis and transport from nucleus to cytoplasm of 28S, 18S and 4S fractions of RNA.", "PMID": 973432}
{"id": "pubmed23n0032_9269", "title": "Attempt at simplification of the rosette -- inhibition test for routine use in patients under immunosuppressive therapy.", "content": "Three hundred thirty three specimens of diluted sera from 69 patients under immunosuppressive therapy were tested for their ability to inhibit rosetting of normal human lymphocytes with SRBC. Statistical analysis of the results of the rosette-inhibition test have shown that there is a relationship of second degree between the inhibition index value and the dilution of serum. This relationship allowed to establish a formula for calculation of the theoretical titer (TT) from one arbitrally chosen dilution of serum. The results of empirical verification of the method are highly satisfactory. The theoretical and empirical titers of 23 tested sera were statistically identical. The proposed method shortens sixfold the time of testing.", "contents": "Attempt at simplification of the rosette -- inhibition test for routine use in patients under immunosuppressive therapy. Three hundred thirty three specimens of diluted sera from 69 patients under immunosuppressive therapy were tested for their ability to inhibit rosetting of normal human lymphocytes with SRBC. Statistical analysis of the results of the rosette-inhibition test have shown that there is a relationship of second degree between the inhibition index value and the dilution of serum. This relationship allowed to establish a formula for calculation of the theoretical titer (TT) from one arbitrally chosen dilution of serum. The results of empirical verification of the method are highly satisfactory. The theoretical and empirical titers of 23 tested sera were statistically identical. The proposed method shortens sixfold the time of testing.", "PMID": 973433}
{"id": "pubmed23n0032_9270", "title": "[The influence of atmospheric gases on growth and toxin production of sterigmatocystin-and patulin-producing molds (author's transl)].", "content": "The influence of one-and two-sided regulated atmospheres with lower 02-and increased CO2-concentrations in comparison with air was studied on the growth and toxin-production of 4 different mycotoxin-producing molds (A. versicolor, P. expansum, P. urticae, B. nivea). Low O2-values in the atmosphere (2-0.5%) had scarely an influence on the development and toxinproduction in different nutrient media after 14 d at 25 degrees C. Only higher CO2-and lower O2-concentrations than that of the air (e.g. 40% CO2, 6% O2) reduced the sterigmatocystin-and patulin-production in nearly all cultures, whereas growth was only retarded insignificantly. Atmospheres with 90% CO2 and 10% air as well nitrogen atmospheres with 10% CO2 inhibited remarkably the growth of molds and prevented the toxin-production of nearly all strains. In pure N2-atmospheres most of the A. versicolor strains exhibited growth with toxic mycelia whereas only minimum growth of the patulin-producing species without any patulin synthesis had been observed.", "contents": "[The influence of atmospheric gases on growth and toxin production of sterigmatocystin-and patulin-producing molds (author's transl)]. The influence of one-and two-sided regulated atmospheres with lower 02-and increased CO2-concentrations in comparison with air was studied on the growth and toxin-production of 4 different mycotoxin-producing molds (A. versicolor, P. expansum, P. urticae, B. nivea). Low O2-values in the atmosphere (2-0.5%) had scarely an influence on the development and toxinproduction in different nutrient media after 14 d at 25 degrees C. Only higher CO2-and lower O2-concentrations than that of the air (e.g. 40% CO2, 6% O2) reduced the sterigmatocystin-and patulin-production in nearly all cultures, whereas growth was only retarded insignificantly. Atmospheres with 90% CO2 and 10% air as well nitrogen atmospheres with 10% CO2 inhibited remarkably the growth of molds and prevented the toxin-production of nearly all strains. In pure N2-atmospheres most of the A. versicolor strains exhibited growth with toxic mycelia whereas only minimum growth of the patulin-producing species without any patulin synthesis had been observed.", "PMID": 973434}
{"id": "pubmed23n0032_9271", "title": "[Investigations of Aflatoxin B1, B2, G1, G2, and M1 in milk and milk products (author's transl)].", "content": "The method proposed by the Federal Department of Health for the determination of aflatoxin B1,B2,G1, and G2 was tested for additional determination of aflatoxin M1. With relatively small changes of the original method, all aflatoxins including. B1, B2,G1,g2, and M1 can be determined quantitatively in milk, milk powder, butter, cheese, quark, cream, yoghurt and fruit yoghurt.", "contents": "[Investigations of Aflatoxin B1, B2, G1, G2, and M1 in milk and milk products (author's transl)]. The method proposed by the Federal Department of Health for the determination of aflatoxin B1,B2,G1, and G2 was tested for additional determination of aflatoxin M1. With relatively small changes of the original method, all aflatoxins including. B1, B2,G1,g2, and M1 can be determined quantitatively in milk, milk powder, butter, cheese, quark, cream, yoghurt and fruit yoghurt.", "PMID": 973435}
{"id": "pubmed23n0032_9272", "title": "Interaction of aflatoxin B1 with some biologically important substances.", "content": "The interaction of aflatoxin B1 with a number of biologically important substances has been investigated by spectrophotometric and fluorimetric techniques. The ultra-violet absorption of tryptophan, adenosine, RNA and DNA were altered by the addition of aflatoxin B1. Some amino-acids, purine and pyrimidine derivatives, bovine-serum albumin and DNA quenched the fluorescence of aflatoxin B1. Fluorescence polarization data suggested that the interaction of aflatoxin B1 with bovine-serum albumin was stronger than that with DNA.", "contents": "Interaction of aflatoxin B1 with some biologically important substances. The interaction of aflatoxin B1 with a number of biologically important substances has been investigated by spectrophotometric and fluorimetric techniques. The ultra-violet absorption of tryptophan, adenosine, RNA and DNA were altered by the addition of aflatoxin B1. Some amino-acids, purine and pyrimidine derivatives, bovine-serum albumin and DNA quenched the fluorescence of aflatoxin B1. Fluorescence polarization data suggested that the interaction of aflatoxin B1 with bovine-serum albumin was stronger than that with DNA.", "PMID": 973436}
{"id": "pubmed23n0032_9273", "title": "Inhibition of growth and aflatoxin production of Aspergillus parasiticus by citrus oils.", "content": "Aspergillus parasiticus was inoculated into grapefruit juice and a glucose-yeast extract medium; both contained 500-7000 ppm of citrus oils that were incorporated into the media by sonication. Orange and lemon oil were more inhibitory to mold growth and aflatoxin production than was dlimonene, the main constituent of the two peel oils. After 7 days at 28 degrees C, 2000 ppm of lemon and 3000 ppm of orange oil in grapefruit juice afforded maximum suppression of mold growth and toxin formation. When the glucose-yeast extract medium was used, 3000 ppm of either oil were needed to achieve the same result. After 4 days at 28 degrees C, orange oil at 3500 ppm in either medium markedly inhibited mold growth (as evidenced by dry weight of mold mycelium) and aflatoxin production (only 14 and 1% of the amount normally produced in the juice and artificial medium, respectively). Higher concentrations of orange oil further reduced mold growth and aflatoxin production and also delayed the onset of sporulation, if it occurred. Although aflatoxin was detected in all samples, only 0.2 to 0.5% of the amount found in controls (without the citrus oil) was present when the medium contained 7000 ppm orange oil. The mold consistently grew, albeit very poorly, on the glass at the liquid-atmosphere interface even when the substrate contained a large amount of citrus oil.", "contents": "Inhibition of growth and aflatoxin production of Aspergillus parasiticus by citrus oils. Aspergillus parasiticus was inoculated into grapefruit juice and a glucose-yeast extract medium; both contained 500-7000 ppm of citrus oils that were incorporated into the media by sonication. Orange and lemon oil were more inhibitory to mold growth and aflatoxin production than was dlimonene, the main constituent of the two peel oils. After 7 days at 28 degrees C, 2000 ppm of lemon and 3000 ppm of orange oil in grapefruit juice afforded maximum suppression of mold growth and toxin formation. When the glucose-yeast extract medium was used, 3000 ppm of either oil were needed to achieve the same result. After 4 days at 28 degrees C, orange oil at 3500 ppm in either medium markedly inhibited mold growth (as evidenced by dry weight of mold mycelium) and aflatoxin production (only 14 and 1% of the amount normally produced in the juice and artificial medium, respectively). Higher concentrations of orange oil further reduced mold growth and aflatoxin production and also delayed the onset of sporulation, if it occurred. Although aflatoxin was detected in all samples, only 0.2 to 0.5% of the amount found in controls (without the citrus oil) was present when the medium contained 7000 ppm orange oil. The mold consistently grew, albeit very poorly, on the glass at the liquid-atmosphere interface even when the substrate contained a large amount of citrus oil.", "PMID": 973437}
{"id": "pubmed23n0032_9274", "title": "[Breakdown of linoeic acid hydroperoxydes. Formation of volatile carbonyl compounds (author's transl)].", "content": "Linoleic acid hydroperoxydes (LOOH) containing 13-hydroperoxyoctadeca-9,11-(75%) and 9-hydroperoxyoctadeca-10,12-dienoic acid (25%) were emulsified at pH 6.5. After addition of hemoglobin, ferrous ions, ferric ions, cysteine or ascorbic acid the emulsions were stored 19 hours at 22 degrees C. The decrease in the diene and peroxyde concentrations and the formation of volatile carbonyl compounds were analysed. Ferrous ions and ascorbic acid were the strongest producers of volatile carbonyl compounds. In the presence of 10(-3) Mol ascorbic acid 6 mumol volatile aldehydes arise from 75 mumol LOOH. Hexanal (70 mol-%) was the main component of the aldehyde fraction. For plant foodstuffs the significance of the reaction of fatty acids hydroperoxydes with ascorbic acid for the formation of flavour substances is discussed.", "contents": "[Breakdown of linoeic acid hydroperoxydes. Formation of volatile carbonyl compounds (author's transl)]. Linoleic acid hydroperoxydes (LOOH) containing 13-hydroperoxyoctadeca-9,11-(75%) and 9-hydroperoxyoctadeca-10,12-dienoic acid (25%) were emulsified at pH 6.5. After addition of hemoglobin, ferrous ions, ferric ions, cysteine or ascorbic acid the emulsions were stored 19 hours at 22 degrees C. The decrease in the diene and peroxyde concentrations and the formation of volatile carbonyl compounds were analysed. Ferrous ions and ascorbic acid were the strongest producers of volatile carbonyl compounds. In the presence of 10(-3) Mol ascorbic acid 6 mumol volatile aldehydes arise from 75 mumol LOOH. Hexanal (70 mol-%) was the main component of the aldehyde fraction. For plant foodstuffs the significance of the reaction of fatty acids hydroperoxydes with ascorbic acid for the formation of flavour substances is discussed.", "PMID": 973438}
{"id": "pubmed23n0032_9275", "title": "[On the occurence of isopeptide bonds in heated milk protein (author's transl)].", "content": "Milk, milk products and individual milk proteins were subjected to different heat treatments either as powders or in aquous systems. After complete hydrolysis of the peptide bonds (alpha-amide bonds) by a system of four proteinases or peptidases, respectively, the samples were analysed for isopeptides. For this purpose, two chromatographic ion exchange systems were developed, each of which separates Nepsilon-(beta-aspartyl-)lysine (Asp Lys) as well as Nepsilon-(gamma-glutamyl-)lysine (Glu Lys) from the common amino acids. In samples, heated 24 h for at least 120 degrees C, 2-5% of the lysine residues are incorporated in Glu Lys-bonds. Under the heating conditions used in dairy practice, no isopeptide bonds were formed.", "contents": "[On the occurence of isopeptide bonds in heated milk protein (author's transl)]. Milk, milk products and individual milk proteins were subjected to different heat treatments either as powders or in aquous systems. After complete hydrolysis of the peptide bonds (alpha-amide bonds) by a system of four proteinases or peptidases, respectively, the samples were analysed for isopeptides. For this purpose, two chromatographic ion exchange systems were developed, each of which separates Nepsilon-(beta-aspartyl-)lysine (Asp Lys) as well as Nepsilon-(gamma-glutamyl-)lysine (Glu Lys) from the common amino acids. In samples, heated 24 h for at least 120 degrees C, 2-5% of the lysine residues are incorporated in Glu Lys-bonds. Under the heating conditions used in dairy practice, no isopeptide bonds were formed.", "PMID": 973439}
{"id": "pubmed23n0032_9276", "title": "[Relations between structure and bitter taste of amino acids and peptides. II. Peptides and their derivatives (author's transl)].", "content": "About 80 peptides and their derivatives were tested for bitter taste. The taste thresholds are in the range of 70-80 muMol/ml (Gly-Val) to 0.01-0.02 muMol/ml (Bacitracin). They are dependent on nature and number of the side chains and on the hydrophobicity of the whole molecule. An estimation of the taste thresholds of all di-and tripeptides with known amino acid composition is possible on the basis of their hydrophobicity. As could be shown recently for bitter amino acids, a polar (electrophilic) and a hydrophobic group are essential requirements for bitter peptides also. This model corresponds to all sensory results, e.g. to the bitter taste of all hydrophobic peptides independent on their sequence and configuration and to the sweet taste of L-aspartyl dipeptide esters.", "contents": "[Relations between structure and bitter taste of amino acids and peptides. II. Peptides and their derivatives (author's transl)]. About 80 peptides and their derivatives were tested for bitter taste. The taste thresholds are in the range of 70-80 muMol/ml (Gly-Val) to 0.01-0.02 muMol/ml (Bacitracin). They are dependent on nature and number of the side chains and on the hydrophobicity of the whole molecule. An estimation of the taste thresholds of all di-and tripeptides with known amino acid composition is possible on the basis of their hydrophobicity. As could be shown recently for bitter amino acids, a polar (electrophilic) and a hydrophobic group are essential requirements for bitter peptides also. This model corresponds to all sensory results, e.g. to the bitter taste of all hydrophobic peptides independent on their sequence and configuration and to the sweet taste of L-aspartyl dipeptide esters.", "PMID": 973440}
{"id": "pubmed23n0032_9277", "title": "The aroma of cloudberries (rubus chamaemorus L.).", "content": "The volatile components of cloudberries (Rubus chamaemorus L.) have been analysed by gas chromatography and mass spectrometry, and about 80 components, comprising 93% of the aroma concentrate, have been identified. The aroma of cloudberries is characterised by the presence of aromatic compounds, which constitute about 53% of the essential oil. The content of terpenes is only 1.5%. Percentage concentrations of the main components are given.", "contents": "The aroma of cloudberries (rubus chamaemorus L.). The volatile components of cloudberries (Rubus chamaemorus L.) have been analysed by gas chromatography and mass spectrometry, and about 80 components, comprising 93% of the aroma concentrate, have been identified. The aroma of cloudberries is characterised by the presence of aromatic compounds, which constitute about 53% of the essential oil. The content of terpenes is only 1.5%. Percentage concentrations of the main components are given.", "PMID": 973441}
{"id": "pubmed23n0032_9278", "title": "[Copper, manganese, and zinc contents in the whole body and in individual parts of veal calves at different weights (author's transl)].", "content": "In a comparative slaughter experiment the copper, manganese and zinc contents in different organ and tissue samples of veal calves at different weights were determined by whole body analyses. For all three trace elements, a decrease of their concentration with increasing age could be observed in the individual parts (blood, adipose tissue, innards, meat, bones) and in the whole body. During the fattening period of 12 weeks the concentrations in the dry matter of the whole body decreased from 12 ppm to 5 ppm copper (P less than 0.001), from 3.5 ppm to 1.6 ppm manganese (P less than 0.05) and from 127 ppm to 69 ppm zinc (P less than 0.05). In the present experiment the animals retained an average of 20 to 22% of the copper intake and about 20% of the manganese intake over the entire feeding period. Zinc was utilized at the extent of 40 to 43%. For the edible parts of the whole body the following coefficients of utilization were obtained: Cu = 10%, Mn = 7%, Zn = 30%.", "contents": "[Copper, manganese, and zinc contents in the whole body and in individual parts of veal calves at different weights (author's transl)]. In a comparative slaughter experiment the copper, manganese and zinc contents in different organ and tissue samples of veal calves at different weights were determined by whole body analyses. For all three trace elements, a decrease of their concentration with increasing age could be observed in the individual parts (blood, adipose tissue, innards, meat, bones) and in the whole body. During the fattening period of 12 weeks the concentrations in the dry matter of the whole body decreased from 12 ppm to 5 ppm copper (P less than 0.001), from 3.5 ppm to 1.6 ppm manganese (P less than 0.05) and from 127 ppm to 69 ppm zinc (P less than 0.05). In the present experiment the animals retained an average of 20 to 22% of the copper intake and about 20% of the manganese intake over the entire feeding period. Zinc was utilized at the extent of 40 to 43%. For the edible parts of the whole body the following coefficients of utilization were obtained: Cu = 10%, Mn = 7%, Zn = 30%.", "PMID": 973442}
{"id": "pubmed23n0032_9279", "title": "A chemical assay method for the determination of aflatoxin residues in animal tissues.", "content": "A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4+1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel--acidic alumina--anhydrous sodium sulfate column chromatography.", "contents": "A chemical assay method for the determination of aflatoxin residues in animal tissues. A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4+1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel--acidic alumina--anhydrous sodium sulfate column chromatography.", "PMID": 973443}
{"id": "pubmed23n0032_9280", "title": "[The characteristics of wheat grains. I. Exterior characteristics of the wheat grains as criterion of a type estimation (author's transl)].", "content": "Typical characteristics of the wheat types Kranich, Kormoran, Kolibri, Diplomat, Benno, Huntsman were worked out and a method for their fast estimation was developed. The characteristics of the different types were drawn in lines. Their usefulness for the estimation of wheat types out of a grain mixture of pure wheat types of unknown age and origin were successfully tested.", "contents": "[The characteristics of wheat grains. I. Exterior characteristics of the wheat grains as criterion of a type estimation (author's transl)]. Typical characteristics of the wheat types Kranich, Kormoran, Kolibri, Diplomat, Benno, Huntsman were worked out and a method for their fast estimation was developed. The characteristics of the different types were drawn in lines. Their usefulness for the estimation of wheat types out of a grain mixture of pure wheat types of unknown age and origin were successfully tested.", "PMID": 973444}
{"id": "pubmed23n0032_9281", "title": "[Flavonols and flavones of vegetables. VII. Flavonols of leek, chive and garlic (author's transl)].", "content": "Green leaves of leek and chive mainly contain kaempferol glycosides, with mono- and di-glycosides dominating in leek and di- and tri-glycosides in chive. In leek glucose is dominant as sugar component compared to xylose; in chive we found glucose and galactose. Kaempferol-3-beta-D-glucoside and kaempferol-3-xylosyl-beta-D-glucoside were isolated from leek and the 3-beta-D-glucosides of kaempferol, quercetin and isorhamnetin as by-glycosides from chive. In leek traces of quercetin-3-glucoside were identified by tlc, but no spiraeoside (quercetin-4'-glucoside) could be detected in the two species. The bulbs of garlic and leek contain only few milligram of glycosides of kaempferol and quercetin per kg fresh weight.", "contents": "[Flavonols and flavones of vegetables. VII. Flavonols of leek, chive and garlic (author's transl)]. Green leaves of leek and chive mainly contain kaempferol glycosides, with mono- and di-glycosides dominating in leek and di- and tri-glycosides in chive. In leek glucose is dominant as sugar component compared to xylose; in chive we found glucose and galactose. Kaempferol-3-beta-D-glucoside and kaempferol-3-xylosyl-beta-D-glucoside were isolated from leek and the 3-beta-D-glucosides of kaempferol, quercetin and isorhamnetin as by-glycosides from chive. In leek traces of quercetin-3-glucoside were identified by tlc, but no spiraeoside (quercetin-4'-glucoside) could be detected in the two species. The bulbs of garlic and leek contain only few milligram of glycosides of kaempferol and quercetin per kg fresh weight.", "PMID": 973445}
{"id": "pubmed23n0032_9282", "title": "Spectrophotometric method for determining the pigment content of ground paprika.", "content": "A rapid and reliable method has been developed for determining the red, yellow and total pigment contents of ground paprika.", "contents": "Spectrophotometric method for determining the pigment content of ground paprika. A rapid and reliable method has been developed for determining the red, yellow and total pigment contents of ground paprika.", "PMID": 973446}
{"id": "pubmed23n0032_9283", "title": "[Gaschromatographical determination of diacetyl, acetoin, and 2,3-pentanedione in wine (author's transl)].", "content": "The determination of diacetyl, 2,3-pentanedione and acetoin was performed in two steps. Diacetyl and 2.3-pentanedione were driven off with gaseous nitrogen at 60 degrees C and collected in ice cooled methanol. The quantitative gaschromatographical determination follows after directly injecting this solution into the gas chromatograph employing an electron capture detector which indicates selectively the vicinale diketones. In a second experiment acetoin was determined by a differential method after oxidizing acetoin to diacetyl. The investigation of 57 German white wines of various vintages, varieties and quality classes yielded the following results: Diacetyl: Limits of variability (V) = 0.08-3.40 mg/l, average amount (M) = 0.42 mg/l; 2.3-pentanedione: V = 0.02-0.36 mg/l, M = 0.10 mg/l; Acetoin: V = 1.9-31.7 mg/l, M = 5.9 mg/l. No differences could be observed with regard to the various quality classes. In 20 red vines of various European origines the following amounts were observed: Diacetyl: V = 0.26-4.06 mg/l, M = 1.46 mg/l; 2.3-pentanedione: V = 0.08-0.88 mg/l, M = 0.25 mg/l; Acetoin: V = 5.9-38.2 mg/l, M = 15.0 mg/l. For a rule the red wines show considerable higher contents of diacetyl, 2.3-pentanedione and acetoin than the white wines.", "contents": "[Gaschromatographical determination of diacetyl, acetoin, and 2,3-pentanedione in wine (author's transl)]. The determination of diacetyl, 2,3-pentanedione and acetoin was performed in two steps. Diacetyl and 2.3-pentanedione were driven off with gaseous nitrogen at 60 degrees C and collected in ice cooled methanol. The quantitative gaschromatographical determination follows after directly injecting this solution into the gas chromatograph employing an electron capture detector which indicates selectively the vicinale diketones. In a second experiment acetoin was determined by a differential method after oxidizing acetoin to diacetyl. The investigation of 57 German white wines of various vintages, varieties and quality classes yielded the following results: Diacetyl: Limits of variability (V) = 0.08-3.40 mg/l, average amount (M) = 0.42 mg/l; 2.3-pentanedione: V = 0.02-0.36 mg/l, M = 0.10 mg/l; Acetoin: V = 1.9-31.7 mg/l, M = 5.9 mg/l. No differences could be observed with regard to the various quality classes. In 20 red vines of various European origines the following amounts were observed: Diacetyl: V = 0.26-4.06 mg/l, M = 1.46 mg/l; 2.3-pentanedione: V = 0.08-0.88 mg/l, M = 0.25 mg/l; Acetoin: V = 5.9-38.2 mg/l, M = 15.0 mg/l. For a rule the red wines show considerable higher contents of diacetyl, 2.3-pentanedione and acetoin than the white wines.", "PMID": 973447}
{"id": "pubmed23n0032_9284", "title": "[Reaction of dihydroxyacetone with methylammoniumacetate. Studies on the maillard-reaction. X. (author's transl)].", "content": "By heating dihydroxyacetone and methylammoniumacetate in aqueous solution, a deeply brown coloured reaction mixture is obtained. The products extractable with methylenechlorid were separated. Several heterocyclic compounds (1-30) could be identified.", "contents": "[Reaction of dihydroxyacetone with methylammoniumacetate. Studies on the maillard-reaction. X. (author's transl)]. By heating dihydroxyacetone and methylammoniumacetate in aqueous solution, a deeply brown coloured reaction mixture is obtained. The products extractable with methylenechlorid were separated. Several heterocyclic compounds (1-30) could be identified.", "PMID": 973448}
{"id": "pubmed23n0032_9285", "title": "Formation of odorous compounds from hydrogen sulphide and 2-butenal.", "content": "Strongly odorous compounds may be formed upon addition of hydrogen sulphide to unsaturated carbonyl compounds. The reaction between hydrogen sulphide and 2-butenal was studied, and the characteristics of the flavour, the structure and some of the physical chemical characteristics of the reaction products were determined. The influence of the pH on the type of components formed in dilute aqueous solution was investigated.", "contents": "Formation of odorous compounds from hydrogen sulphide and 2-butenal. Strongly odorous compounds may be formed upon addition of hydrogen sulphide to unsaturated carbonyl compounds. The reaction between hydrogen sulphide and 2-butenal was studied, and the characteristics of the flavour, the structure and some of the physical chemical characteristics of the reaction products were determined. The influence of the pH on the type of components formed in dilute aqueous solution was investigated.", "PMID": 973449}
{"id": "pubmed23n0032_9286", "title": "[Polarografic determination of saccharin in beverages and foods (author's transl)].", "content": "A method for polarografic determination of saccharin in beverages and foods is described. Saccharin is extracted from an acid medium, after clean up with petr. ether, with aether-petr. aether (30/70). Minimum doses which can be quantitatively determined are 5 ppm of saccharin.", "contents": "[Polarografic determination of saccharin in beverages and foods (author's transl)]. A method for polarografic determination of saccharin in beverages and foods is described. Saccharin is extracted from an acid medium, after clean up with petr. ether, with aether-petr. aether (30/70). Minimum doses which can be quantitatively determined are 5 ppm of saccharin.", "PMID": 973450}
{"id": "pubmed23n0032_9287", "title": "The detection of chicken meat in meat products by means of the anserine/carnosine ratio.", "content": "A distinctive difference was found between the ratio of the anserine and carnosine contents (a/c ratio) in beef or pork and of that in chicken/meat. The a/c ratio for beef varies between 0.06-0.2 and for pork between 0.02-0.1 but for chicken meat can reach values as high as 2.2-5.5. The high a/c ratio for chicken meat proved to be sufficient to detect this ingredient at a 5% level in both cooked pork products and 1:1 beef-pork mixtures, this being independent of the heat treatment. The a/c ratio should be considered to be a suitable parameter for the presence of chicken meat in meat products.", "contents": "The detection of chicken meat in meat products by means of the anserine/carnosine ratio. A distinctive difference was found between the ratio of the anserine and carnosine contents (a/c ratio) in beef or pork and of that in chicken/meat. The a/c ratio for beef varies between 0.06-0.2 and for pork between 0.02-0.1 but for chicken meat can reach values as high as 2.2-5.5. The high a/c ratio for chicken meat proved to be sufficient to detect this ingredient at a 5% level in both cooked pork products and 1:1 beef-pork mixtures, this being independent of the heat treatment. The a/c ratio should be considered to be a suitable parameter for the presence of chicken meat in meat products.", "PMID": 973451}
{"id": "pubmed23n0032_9288", "title": "[Lead- and cadmium-content of foodstuffs. 2. Lead- and cadmium-content of commercially prepared soups (authors transl)].", "content": "The lead and cadmium contents in 30 commercially prepared soup products (soup powders and cubes) of several producers were determined. The average lead value was 0.28 ppm. 29 samples contained between 0,095 ppm and 0.45 ppm, whereas in 1 product 1.4 ppm was found. The average cadmium concentration in 21 products was 0.025 ppm (highest value 0.052 ppm). In the other samples cadmium was not found (less than 0.004 ppm Cd).", "contents": "[Lead- and cadmium-content of foodstuffs. 2. Lead- and cadmium-content of commercially prepared soups (authors transl)]. The lead and cadmium contents in 30 commercially prepared soup products (soup powders and cubes) of several producers were determined. The average lead value was 0.28 ppm. 29 samples contained between 0,095 ppm and 0.45 ppm, whereas in 1 product 1.4 ppm was found. The average cadmium concentration in 21 products was 0.025 ppm (highest value 0.052 ppm). In the other samples cadmium was not found (less than 0.004 ppm Cd).", "PMID": 973452}
{"id": "pubmed23n0032_9289", "title": "[Formation of pyridone derivates from maltose and lactose. XII. Investigations on the Maillard-reaction (authors transl)].", "content": "Maltose and lactose react with methylammoniumacetate in a hot aqueous solution giving a dark brown mixture of products. 1,2-dimethyl-3-hydroxy-4-pyridone (5) can be isolated from the volatile compounds. Maltol and Isomaltol can be converted into the pyridone 5 with methylammoniumacetate. 1-Carboxymethyl-3-hydroxy-2-methyl-4-pyridone is obtained from isomaltol and glycine.", "contents": "[Formation of pyridone derivates from maltose and lactose. XII. Investigations on the Maillard-reaction (authors transl)]. Maltose and lactose react with methylammoniumacetate in a hot aqueous solution giving a dark brown mixture of products. 1,2-dimethyl-3-hydroxy-4-pyridone (5) can be isolated from the volatile compounds. Maltol and Isomaltol can be converted into the pyridone 5 with methylammoniumacetate. 1-Carboxymethyl-3-hydroxy-2-methyl-4-pyridone is obtained from isomaltol and glycine.", "PMID": 973453}
{"id": "pubmed23n0032_9290", "title": "[The phenolics of fruits. VIII. Changes in flavonol concentrations during fruit development (authors transl)].", "content": "In black currants the contents of quercetin- and especially of myricetin glycosides increased during ripeness very considerably. The myricetin level in ripe fruits of cultivated blueberries and the quercetin level in ripe berries of privet was higher than in unripe. However, the concentrations of kaempferol and quercetin glycosides were in most of the fruits (e.g. red and white currants, sour cherries, plums, cultivated blueberries, elderberries) smaller than in unripe fruits. Anthocyanin formation is therefore not combined with an accumulation of flavonols (perhaps with the exception of myricetin).", "contents": "[The phenolics of fruits. VIII. Changes in flavonol concentrations during fruit development (authors transl)]. In black currants the contents of quercetin- and especially of myricetin glycosides increased during ripeness very considerably. The myricetin level in ripe fruits of cultivated blueberries and the quercetin level in ripe berries of privet was higher than in unripe. However, the concentrations of kaempferol and quercetin glycosides were in most of the fruits (e.g. red and white currants, sour cherries, plums, cultivated blueberries, elderberries) smaller than in unripe fruits. Anthocyanin formation is therefore not combined with an accumulation of flavonols (perhaps with the exception of myricetin).", "PMID": 973454}
{"id": "pubmed23n0032_9291", "title": "[Flavonols and flavones of vegetables. VI. On the changes of the flavonols of onions (authors transl)].", "content": "The epidermis of onion scales exclusively contains glucosides of quercetin as flavonols, while the dry outer skins contain quercetin in the free state mainly. First spiraeoside (quercetin-4'-glucoside) is built; the formation of diglucosides follows during storage and increases continously. The mesophyll appears to be free of flavonols. The flavonol concentration decreases from the outer to the inner scales, with higher levels in the outer than in the inner epidermis. In the green leaves the flavonol synthesis is light-dependent and also kaempferol glucosides are formed beside quercetin glucosides, but not spiraeoside and the known diglucosides of the scales. During drying on the field an accumulation of flavonols takes place in the drying leaves. This production is associated with the formation of free quercetin and spiraeoside, but not of free kaempferol.", "contents": "[Flavonols and flavones of vegetables. VI. On the changes of the flavonols of onions (authors transl)]. The epidermis of onion scales exclusively contains glucosides of quercetin as flavonols, while the dry outer skins contain quercetin in the free state mainly. First spiraeoside (quercetin-4'-glucoside) is built; the formation of diglucosides follows during storage and increases continously. The mesophyll appears to be free of flavonols. The flavonol concentration decreases from the outer to the inner scales, with higher levels in the outer than in the inner epidermis. In the green leaves the flavonol synthesis is light-dependent and also kaempferol glucosides are formed beside quercetin glucosides, but not spiraeoside and the known diglucosides of the scales. During drying on the field an accumulation of flavonols takes place in the drying leaves. This production is associated with the formation of free quercetin and spiraeoside, but not of free kaempferol.", "PMID": 973455}
{"id": "pubmed23n0032_9292", "title": "[Studies of coffee and coffee-substitute. XVIII. Dependence of the quantity and composition of a high polymer galactomannan on the coffee species and degree of roasting (authors transl)].", "content": "It is possible to determine quantitatively with satisfactory accuracy the galactomannan which is precipitated in coffee brew after addition of Fehling solution. The content of polysaccharides in the extracts increases with the degree of roasting. Galactomannan makes up 6.6 to 12.7% of the dry matter of the extracts and 1.8 to 4.4% of that of roast coffee. It consists mainly of mannan, to a much smaller degree of galactan. Often there are traces of glucan. The content of mannan increases with the degree of roasting while that of galactan decreases very rapidly.", "contents": "[Studies of coffee and coffee-substitute. XVIII. Dependence of the quantity and composition of a high polymer galactomannan on the coffee species and degree of roasting (authors transl)]. It is possible to determine quantitatively with satisfactory accuracy the galactomannan which is precipitated in coffee brew after addition of Fehling solution. The content of polysaccharides in the extracts increases with the degree of roasting. Galactomannan makes up 6.6 to 12.7% of the dry matter of the extracts and 1.8 to 4.4% of that of roast coffee. It consists mainly of mannan, to a much smaller degree of galactan. Often there are traces of glucan. The content of mannan increases with the degree of roasting while that of galactan decreases very rapidly.", "PMID": 973456}
{"id": "pubmed23n0032_9293", "title": "[Sensory and chemical changes of dried foods under the influence of extreme storage conditions. 3. Chemical changes (authors transl)].", "content": "The content of free amino acids and free fatty acids of dried infant foods is clearly decreasing during the storage under extreme conditions, whilst the percentage composition of the fatty acids in the triglycerides remains nearly unchanged. The content of hydroxymethylfurfural is connected with the sensory changes. For the single foods as well a high increase as a strong decrease of the HMF-content can be combined with distinct sensory changes. The vitamin C-content decreases during the storage, the permeability of the packing material for oxygen and water vapor has an unfavourable effect. Furthermore the pH-value of the investigated products is lowered during the storage period.", "contents": "[Sensory and chemical changes of dried foods under the influence of extreme storage conditions. 3. Chemical changes (authors transl)]. The content of free amino acids and free fatty acids of dried infant foods is clearly decreasing during the storage under extreme conditions, whilst the percentage composition of the fatty acids in the triglycerides remains nearly unchanged. The content of hydroxymethylfurfural is connected with the sensory changes. For the single foods as well a high increase as a strong decrease of the HMF-content can be combined with distinct sensory changes. The vitamin C-content decreases during the storage, the permeability of the packing material for oxygen and water vapor has an unfavourable effect. Furthermore the pH-value of the investigated products is lowered during the storage period.", "PMID": 973457}
{"id": "pubmed23n0032_9294", "title": "[Sensory and chemical changes of dried foods under the influence of extreme storage conditions. 4. Flavor compounds (authors transl)].", "content": "From dried infant foods stored under extreme conditions aroma compounds have been isolated analyzed by gas chromatography and mass spectrometry. The results has been, that during the storage long chain aliphatic aldehydes are newly formed.--It is scarcely possible to attach the investigated chemical parameters (content of ascorbic acid, free amino acids and free fatty acids, hydroxymethylfurfural, pH-value) for the investigated products to a sensory impression.", "contents": "[Sensory and chemical changes of dried foods under the influence of extreme storage conditions. 4. Flavor compounds (authors transl)]. From dried infant foods stored under extreme conditions aroma compounds have been isolated analyzed by gas chromatography and mass spectrometry. The results has been, that during the storage long chain aliphatic aldehydes are newly formed.--It is scarcely possible to attach the investigated chemical parameters (content of ascorbic acid, free amino acids and free fatty acids, hydroxymethylfurfural, pH-value) for the investigated products to a sensory impression.", "PMID": 973458}
{"id": "pubmed23n0032_9295", "title": "Reaction of hydrogen sulphide with 2-alkenals.", "content": "Depending on the types of solvent and catalyst, different products are formed in the reaction of hydrogen sulphide with 2-alkenals. The structures of many of these products have been elucidated, and the reaction sequences leading to them are proposed.", "contents": "Reaction of hydrogen sulphide with 2-alkenals. Depending on the types of solvent and catalyst, different products are formed in the reaction of hydrogen sulphide with 2-alkenals. The structures of many of these products have been elucidated, and the reaction sequences leading to them are proposed.", "PMID": 973459}
{"id": "pubmed23n0032_9296", "title": "Formation of a derivative of aflatoxin B1 on a sephadex column with a 1% aqueous solution of methanol.", "content": "A PARTIAL SEPARATION OF AFLATOXINS B1 and B2 on a Sephadex column with a 1% aqueous solution of methanol as mobile phase was achieved. The chromatographic system is, however, not neutral for aflatoxin B1. During elution a derivative--probably aflatoxin B1 hemiacetal-is produced. The derivative forms blue fluorescent spots on Adsorbosil-1 plates with Rf 0.15 when developed in chloroform/acetone (90 + 10).", "contents": "Formation of a derivative of aflatoxin B1 on a sephadex column with a 1% aqueous solution of methanol. A PARTIAL SEPARATION OF AFLATOXINS B1 and B2 on a Sephadex column with a 1% aqueous solution of methanol as mobile phase was achieved. The chromatographic system is, however, not neutral for aflatoxin B1. During elution a derivative--probably aflatoxin B1 hemiacetal-is produced. The derivative forms blue fluorescent spots on Adsorbosil-1 plates with Rf 0.15 when developed in chloroform/acetone (90 + 10).", "PMID": 973460}
{"id": "pubmed23n0032_9297", "title": "[On the occurrence of proanthocyanidins, leucoanthocyanidins and catechins in vegetables (author's transl)].", "content": "22 species of vegetables grown in Germany were investigated and only rhubarb contained (+)-catechin besides traces of (-)-epicatechin. The catechin concentrations were lower in the stalks than in the leaves and decreased during plant growth. Proanthocyanidins (dimers and oligomers of polyhydroxy-flavan-3-ols) were only found in the testa of broad beans, beans, and peas with coloured flowers, and in rhubarb stalks. Leucoanthocyanidins (polyhydroxy-flavan-3,4-diols) could not be found in any vegetable.", "contents": "[On the occurrence of proanthocyanidins, leucoanthocyanidins and catechins in vegetables (author's transl)]. 22 species of vegetables grown in Germany were investigated and only rhubarb contained (+)-catechin besides traces of (-)-epicatechin. The catechin concentrations were lower in the stalks than in the leaves and decreased during plant growth. Proanthocyanidins (dimers and oligomers of polyhydroxy-flavan-3-ols) were only found in the testa of broad beans, beans, and peas with coloured flowers, and in rhubarb stalks. Leucoanthocyanidins (polyhydroxy-flavan-3,4-diols) could not be found in any vegetable.", "PMID": 973461}
{"id": "pubmed23n0032_9298", "title": "[GLC-mass-spectrometrical investigation of the volatile components of wines. VI. Aroma compounds of Tokaj aszu wines. a) Neutral compounds (author's transl)].", "content": "The aroma compounds of Tokaj aszu-wines (1963, 5-butt. and 1973, 6-butt.) have been enriched by liquid-liquid extraction (pentane-methylenchloride 2 + 1) and compared with the aroma substances of a magyar Furmint wine (1973). After the separation of the acids and after the fractionation of the aroma extracts on silicagel 119 aroma components have been identified in the neutral fractions by means of the combination gaschromatography--mass spectrometry. Concerning the volatile constituents relatively high concentrations of furfural, 5-methyl-furfural, acetoin, different acetals, the ethyl esters of keto-, hydroxy- and dicarbonic acids and the lack of terpenic compounds are characteristic of the aszu wines. Quantitative differences depending on the vintages and the conditions of storage have been determined; particularly the concentrations of the acetales depend on the age of the wines.", "contents": "[GLC-mass-spectrometrical investigation of the volatile components of wines. VI. Aroma compounds of Tokaj aszu wines. a) Neutral compounds (author's transl)]. The aroma compounds of Tokaj aszu-wines (1963, 5-butt. and 1973, 6-butt.) have been enriched by liquid-liquid extraction (pentane-methylenchloride 2 + 1) and compared with the aroma substances of a magyar Furmint wine (1973). After the separation of the acids and after the fractionation of the aroma extracts on silicagel 119 aroma components have been identified in the neutral fractions by means of the combination gaschromatography--mass spectrometry. Concerning the volatile constituents relatively high concentrations of furfural, 5-methyl-furfural, acetoin, different acetals, the ethyl esters of keto-, hydroxy- and dicarbonic acids and the lack of terpenic compounds are characteristic of the aszu wines. Quantitative differences depending on the vintages and the conditions of storage have been determined; particularly the concentrations of the acetales depend on the age of the wines.", "PMID": 973462}
{"id": "pubmed23n0032_9299", "title": "[On the metabolism of amino acids by lactic acid bacteria isolated from wine (author's transl)].", "content": "The changes in the concentrations of amino acids in the culture medium of lactic acid bacteria were determined by ion exchange chromatography after growth of 105 strains that were mainly isolated from wine. After growth of lactic acid bacteria a small but not significant decrease in the concentration of most amino acids is observed. There is apparently no difference between essential or not essential amino acids. Certain bacterial strains decompose the amino acids arginine, glutamic acid, histidine, and tyrosine completely. Other amino acids (tryptophane, aspartic acid, threonine, isoleucine, and phenylalanine) are only partially metabolized. Among 28 strains of Pediococcus cerevisiae only one strain was able to decarboxylate histidine to histamine. This was the only strain found to have this ability. Several strains of Lactobacillus brevis were able to form 4-aminobutyric acid and ornithine from glutamic acid and arginine respectively.", "contents": "[On the metabolism of amino acids by lactic acid bacteria isolated from wine (author's transl)]. The changes in the concentrations of amino acids in the culture medium of lactic acid bacteria were determined by ion exchange chromatography after growth of 105 strains that were mainly isolated from wine. After growth of lactic acid bacteria a small but not significant decrease in the concentration of most amino acids is observed. There is apparently no difference between essential or not essential amino acids. Certain bacterial strains decompose the amino acids arginine, glutamic acid, histidine, and tyrosine completely. Other amino acids (tryptophane, aspartic acid, threonine, isoleucine, and phenylalanine) are only partially metabolized. Among 28 strains of Pediococcus cerevisiae only one strain was able to decarboxylate histidine to histamine. This was the only strain found to have this ability. Several strains of Lactobacillus brevis were able to form 4-aminobutyric acid and ornithine from glutamic acid and arginine respectively.", "PMID": 973463}
{"id": "pubmed23n0032_9300", "title": "Nonenzymic browning. XV. Effect of unsaturation on browning reactions of oxidized lipids with protein.", "content": "The amount of pigments produced by interaction of oxidized lipids with albumin and the intensity of coloration increase with increasing degree of unsaturation of the lipid fraction.", "contents": "Nonenzymic browning. XV. Effect of unsaturation on browning reactions of oxidized lipids with protein. The amount of pigments produced by interaction of oxidized lipids with albumin and the intensity of coloration increase with increasing degree of unsaturation of the lipid fraction.", "PMID": 973464}
{"id": "pubmed23n0032_9301", "title": "[Disc-electrophoresis of the water-soluble proteins of some wheat varieties (author's transl)].", "content": "The method described allows a reliable detection of the variety Kranich out of 9 varieties investigated within 5 h.", "contents": "[Disc-electrophoresis of the water-soluble proteins of some wheat varieties (author's transl)]. The method described allows a reliable detection of the variety Kranich out of 9 varieties investigated within 5 h.", "PMID": 973465}
{"id": "pubmed23n0032_9302", "title": "[Cycloalkylsulfamic acids and their salts. V. (author's transl)].", "content": "3-Methyl-4-thia-cyclohexylsulfamic acid was synthesized, the degree of sweetness of its sodium salt was measured, and compared with that of the sodium salts of 3-methyl-cyclohexylsulfamic acid, 4-thia-cyclohexylsulfamic acid, and cyclohexylsulfamic acid.", "contents": "[Cycloalkylsulfamic acids and their salts. V. (author's transl)]. 3-Methyl-4-thia-cyclohexylsulfamic acid was synthesized, the degree of sweetness of its sodium salt was measured, and compared with that of the sodium salts of 3-methyl-cyclohexylsulfamic acid, 4-thia-cyclohexylsulfamic acid, and cyclohexylsulfamic acid.", "PMID": 973466}
{"id": "pubmed23n0032_9303", "title": "[The evaluation of test panel members with the tetrade-test. Short notice to the contribution of Miller, Schmidt and Wildbrett (author's transl)].", "content": "For the application of the tetrade-test in the evaluation of sensory test panel members some principles are mentioned and some interpretations are given.", "contents": "[The evaluation of test panel members with the tetrade-test. Short notice to the contribution of Miller, Schmidt and Wildbrett (author's transl)]. For the application of the tetrade-test in the evaluation of sensory test panel members some principles are mentioned and some interpretations are given.", "PMID": 973467}
{"id": "pubmed23n0032_9304", "title": "[The problem of treating breast cancer by limited surgery (author's transl)].", "content": "The value of radical mastectomy in treating cancer of the breast has been questioned by some authors. With regard to the results published and on the basis of our own experiences limited operations without or with additional radiotherapy cannot be considered to be reliable procedures at present: it is only possible to approximately equal results by a strict case selection. With special cases such as in-situ carcinoma or small well defined cancers with beginning infiltration being expected there is no real basis for the indication to limited surgery in a single case. With the increase of small prognostically favourable cancers and border-line cases due to improvement of early detection more attention should be devoted to the problem of treating breast cancer by limited operations.", "contents": "[The problem of treating breast cancer by limited surgery (author's transl)]. The value of radical mastectomy in treating cancer of the breast has been questioned by some authors. With regard to the results published and on the basis of our own experiences limited operations without or with additional radiotherapy cannot be considered to be reliable procedures at present: it is only possible to approximately equal results by a strict case selection. With special cases such as in-situ carcinoma or small well defined cancers with beginning infiltration being expected there is no real basis for the indication to limited surgery in a single case. With the increase of small prognostically favourable cancers and border-line cases due to improvement of early detection more attention should be devoted to the problem of treating breast cancer by limited operations.", "PMID": 973479}
{"id": "pubmed23n0032_9305", "title": "[Cholesteatosis of gallbladder (author's transl)].", "content": "741 cholecystektomies were examined for cholesterosis. In 92% gallstones were present and in 8% there were no stones. Cholesterosis of the gallbladder was found in 0,9% of 4417 autopsies. It was combined with gallstones in 25% and in 75% it was not. There was no difference concerning the clinical symptoms between cholesterosis with and without stones in the surgical patients. Cholesterosis without stone presented the same picture as gallstones. In the autopsy material however no typical symptoms could be found indicating either cholesterosis with gallstones or without. In the histological examination 80,5% of the cases with gallstones in the surgical and autopsy group had inflammatory changes of the gallbladder wall whereas only 22% of the cases with stoneless cholesterosis showed these alterations. Fibrosis of the gallbladder wall however was found in 86,1% in cases with cholesterosis without stones and in 88% in cases with stones. From these findings the conclusion is made that cholesterosis is no well defined disease. Clinical symptoms seem to appear only when other factors, such as stones, inflammation or functional disturbances probably because of fibrosis of the wall, become manifest in addition.", "contents": "[Cholesteatosis of gallbladder (author's transl)]. 741 cholecystektomies were examined for cholesterosis. In 92% gallstones were present and in 8% there were no stones. Cholesterosis of the gallbladder was found in 0,9% of 4417 autopsies. It was combined with gallstones in 25% and in 75% it was not. There was no difference concerning the clinical symptoms between cholesterosis with and without stones in the surgical patients. Cholesterosis without stone presented the same picture as gallstones. In the autopsy material however no typical symptoms could be found indicating either cholesterosis with gallstones or without. In the histological examination 80,5% of the cases with gallstones in the surgical and autopsy group had inflammatory changes of the gallbladder wall whereas only 22% of the cases with stoneless cholesterosis showed these alterations. Fibrosis of the gallbladder wall however was found in 86,1% in cases with cholesterosis without stones and in 88% in cases with stones. From these findings the conclusion is made that cholesterosis is no well defined disease. Clinical symptoms seem to appear only when other factors, such as stones, inflammation or functional disturbances probably because of fibrosis of the wall, become manifest in addition.", "PMID": 973480}
{"id": "pubmed23n0032_9306", "title": "[Frequency of carcinoma in a medium-sized country-hospital (author's transl)].", "content": "The author tried to give a broad survey of the cases of carcinoma in a medium-sized hospital during a certain period. The research included the following criteria: frequency, distribution regarding sex and age, localisation of carcinoma, histology, therapeutical methods, kind of metastasia and survival time. The 5 most frequent cases of carcinoma were more closely investigated in detail. The importance of a more intensified early diagnosis of carcinoma for keeping the rate of inoperable cases low is stressed.", "contents": "[Frequency of carcinoma in a medium-sized country-hospital (author's transl)]. The author tried to give a broad survey of the cases of carcinoma in a medium-sized hospital during a certain period. The research included the following criteria: frequency, distribution regarding sex and age, localisation of carcinoma, histology, therapeutical methods, kind of metastasia and survival time. The 5 most frequent cases of carcinoma were more closely investigated in detail. The importance of a more intensified early diagnosis of carcinoma for keeping the rate of inoperable cases low is stressed.", "PMID": 973481}
{"id": "pubmed23n0032_9307", "title": "[The frequency of physiologic vaginal bleeding of newborn infants].", "content": "A study of 350 newborn girls revealed vaginal (uterine) bleeding in 25,4%, mostly demonstrable only by chemical micro-methods, but also macroscopically visible in 3,3%. This kind of bleeding appears always in the first week, with its highest frequency on the 5th day of life and must be regarded as a physiologic event. Analogous histologic and cytologic controls confirmed these observations, pointing toward the fact that, as a rule, no menstrual withdrawal bleeding, but degenerative, regressional changes upon the endometrium occur. Due to the rapid withdrawal of maternal hormones, nutritive disorders may lead also to diapedesis bleeding. But if any genital bleeding lasts longer than one week or appears for the first time after that period, further diagnostic procedures are essential as a possible pathologic condition may be assumed.", "contents": "[The frequency of physiologic vaginal bleeding of newborn infants]. A study of 350 newborn girls revealed vaginal (uterine) bleeding in 25,4%, mostly demonstrable only by chemical micro-methods, but also macroscopically visible in 3,3%. This kind of bleeding appears always in the first week, with its highest frequency on the 5th day of life and must be regarded as a physiologic event. Analogous histologic and cytologic controls confirmed these observations, pointing toward the fact that, as a rule, no menstrual withdrawal bleeding, but degenerative, regressional changes upon the endometrium occur. Due to the rapid withdrawal of maternal hormones, nutritive disorders may lead also to diapedesis bleeding. But if any genital bleeding lasts longer than one week or appears for the first time after that period, further diagnostic procedures are essential as a possible pathologic condition may be assumed.", "PMID": 973490}
{"id": "pubmed23n0032_9308", "title": "[The kinetics of sulfamerazine (Mebacid) and the effect of sulfamerazine therapy on p-aminohippuric acid clearance in pregnancy].", "content": "Elimination half-life of Sulfamerazin (Mebacid) was not different in pregnant and non-pregnant women after a single oral dose and after 11 days repeated administration in therapeutic dosis. The initial concentrations of Sulfamerazin are lower in pregnants than in non-pregnants. Elimination half-life of p-aminohippurate (PAH) is shortened by Sulfamerazin-pretreatment, renal excretion of PAH is increased respectively.", "contents": "[The kinetics of sulfamerazine (Mebacid) and the effect of sulfamerazine therapy on p-aminohippuric acid clearance in pregnancy]. Elimination half-life of Sulfamerazin (Mebacid) was not different in pregnant and non-pregnant women after a single oral dose and after 11 days repeated administration in therapeutic dosis. The initial concentrations of Sulfamerazin are lower in pregnants than in non-pregnants. Elimination half-life of p-aminohippurate (PAH) is shortened by Sulfamerazin-pretreatment, renal excretion of PAH is increased respectively.", "PMID": 973491}
{"id": "pubmed23n0032_9309", "title": "[A central fetal monitoring system in the labor room].", "content": "Report about central obstetrical monitoring system (Biomonitor Kombinat VEB Messger\u00e4tewerk Zw\u00f6nitz). The following bed-side monitors have been used: BMT-504 (Kombinat V-B Messger\u00e4tewerk Zw\u00f6nitz), HP 8020 A (Hewlett Packard), FM-3 (Kranzb\u00fchler). The FECG and CTG or other fetal, maternal and neonatal functions from six single obstetrical rooms have been registrated in a central work room by means of a 6-channel-recorder (6-NEK-4) and a 6-channel-scope (USG-101). The mother and newborn can be supervised by means of television. This central fetal monitoring system gives the following advantages. There is no disturbance of the patient because the recording and warning events takes place in a central work room. The service of the recorders and scopes is simplified. The room-saving arrangement of bed-side monitors on instrument brackets. Recorders and instrument trolleys have been spared. Preparation of CTG computer analysis. The continuous cardiotocography of all labors (2000/year) by means of the central fetal monitoring has been realized with two midwifes and one physician around the clock. The installation of a fetal central monitoring system is for reconstruction or construction of obstetrical departments to take in consideration.", "contents": "[A central fetal monitoring system in the labor room]. Report about central obstetrical monitoring system (Biomonitor Kombinat VEB Messger\u00e4tewerk Zw\u00f6nitz). The following bed-side monitors have been used: BMT-504 (Kombinat V-B Messger\u00e4tewerk Zw\u00f6nitz), HP 8020 A (Hewlett Packard), FM-3 (Kranzb\u00fchler). The FECG and CTG or other fetal, maternal and neonatal functions from six single obstetrical rooms have been registrated in a central work room by means of a 6-channel-recorder (6-NEK-4) and a 6-channel-scope (USG-101). The mother and newborn can be supervised by means of television. This central fetal monitoring system gives the following advantages. There is no disturbance of the patient because the recording and warning events takes place in a central work room. The service of the recorders and scopes is simplified. The room-saving arrangement of bed-side monitors on instrument brackets. Recorders and instrument trolleys have been spared. Preparation of CTG computer analysis. The continuous cardiotocography of all labors (2000/year) by means of the central fetal monitoring has been realized with two midwifes and one physician around the clock. The installation of a fetal central monitoring system is for reconstruction or construction of obstetrical departments to take in consideration.", "PMID": 973492}
{"id": "pubmed23n0032_9310", "title": "[The significance of fetal electroencephalography for the diagnosis of fetal disorders].", "content": "In typical cardiotocographic and bloodgasanalytic changes indicating fetal hypoxia the fetal electroencephalography shows frequency slowing, voltage suppression and spike activity. This EEG tracings simultaneously appeared with first cardiotocographic signs of fetal distress. It was not possible to recognize disturbances of fetal oxygenation in an earlier stage by EEG monitoring. Fetal EEG is of difficult technique and interpretation. In so far cardiotocography is of higher security, sensibility and easier handling for fetal monitoring. With regard to future improvements in electroencephalographic techniques it seems possible to get additional and more accurate informations for fetal monitoring and about perinatal morbidity.", "contents": "[The significance of fetal electroencephalography for the diagnosis of fetal disorders]. In typical cardiotocographic and bloodgasanalytic changes indicating fetal hypoxia the fetal electroencephalography shows frequency slowing, voltage suppression and spike activity. This EEG tracings simultaneously appeared with first cardiotocographic signs of fetal distress. It was not possible to recognize disturbances of fetal oxygenation in an earlier stage by EEG monitoring. Fetal EEG is of difficult technique and interpretation. In so far cardiotocography is of higher security, sensibility and easier handling for fetal monitoring. With regard to future improvements in electroencephalographic techniques it seems possible to get additional and more accurate informations for fetal monitoring and about perinatal morbidity.", "PMID": 973493}
{"id": "pubmed23n0032_9311", "title": "[Comparative studies of the value of impulse-reception methods of prenatal cardiotocography].", "content": "102 pregnant womens have been controlled by means of the various three methods of the antenatal CTG (phonocardiography, ultrasonography and indirect fetal ECG) between the 22th to 41th week. The registered time for each method was ten minutes. Between the 27th to 33th week of the pregnancy the ultrasonography gives the best results. The phonocardiography gives after the 32th week, especially after the 37th week, increasing technical unobjectionable tachograms. There was a good tachografic performans between the 22th to 27th week and after the 35th week by means of indirect fetal ECG. Between the 27th and 34th week this method refuses. The interpretation of indirect fetal ECG or phonocardiotocography, if they are possible, shows clear advantages in antenatal diagnostic of fetal condition.", "contents": "[Comparative studies of the value of impulse-reception methods of prenatal cardiotocography]. 102 pregnant womens have been controlled by means of the various three methods of the antenatal CTG (phonocardiography, ultrasonography and indirect fetal ECG) between the 22th to 41th week. The registered time for each method was ten minutes. Between the 27th to 33th week of the pregnancy the ultrasonography gives the best results. The phonocardiography gives after the 32th week, especially after the 37th week, increasing technical unobjectionable tachograms. There was a good tachografic performans between the 22th to 27th week and after the 35th week by means of indirect fetal ECG. Between the 27th and 34th week this method refuses. The interpretation of indirect fetal ECG or phonocardiotocography, if they are possible, shows clear advantages in antenatal diagnostic of fetal condition.", "PMID": 973494}
{"id": "pubmed23n0032_9312", "title": "[Extreme antepartum fetal tachycardia].", "content": "On the occasion of a case with fetal paroxysmal tachykardia a synopsis is given on former observations of extreme fetal tachycardias ante-partum. Here is our conclusion: Even extreme and long lasting tachycardias alone are not an indication of operative delivery.", "contents": "[Extreme antepartum fetal tachycardia]. On the occasion of a case with fetal paroxysmal tachykardia a synopsis is given on former observations of extreme fetal tachycardias ante-partum. Here is our conclusion: Even extreme and long lasting tachycardias alone are not an indication of operative delivery.", "PMID": 973495}
{"id": "pubmed23n0032_9313", "title": "[Agonal coma in stroke].", "content": "The author describes the clinical symptomatology of coma d\u00e9pass\u00e9 in 19 patients with severe forms of brain strokes. Artificial pulmonary ventilation was performed until cardic arrest. The longest time of dissociated life of the organism in strokes was 5 days. Spinal automatisms were seen in 9 cases. Some possibilities of unreversible disintegration of separate structures in the CNS in different periods of coma d\u00e9pass\u00e9 (and in some cases prior to its development) are discussed.", "contents": "[Agonal coma in stroke]. The author describes the clinical symptomatology of coma d\u00e9pass\u00e9 in 19 patients with severe forms of brain strokes. Artificial pulmonary ventilation was performed until cardic arrest. The longest time of dissociated life of the organism in strokes was 5 days. Spinal automatisms were seen in 9 cases. Some possibilities of unreversible disintegration of separate structures in the CNS in different periods of coma d\u00e9pass\u00e9 (and in some cases prior to its development) are discussed.", "PMID": 973511}
{"id": "pubmed23n0032_9314", "title": "[Semiologic, biochemical and electrophysiologic analysis of the stages of apopleptic coma].", "content": "The paper contains some results of a comprehensive clinical, biochemical and electrophysiological study of 120 patients with different stages of an apoplectic coma. The authors studied the gas content, acid-base balance of the cranial blood inflow and outflow in the CSF and changes of EEG and EMG. It was shown that there were different levels of CNS lesions (cortico-subcortical, meso-diencephalic, meso-pontile and bulbar level) which corresponded to certain correlations between clinical, biochemical, EEG and EMG findings. The authors describe a classificational scheme of 4 stages of an apopletic coma. Special significance in the pathogenesis of a coma along with structural lesions in the different areas of the CNS is attached to metabolic disturbances and extracerebral factors (respiratory and circulatory disorders).", "contents": "[Semiologic, biochemical and electrophysiologic analysis of the stages of apopleptic coma]. The paper contains some results of a comprehensive clinical, biochemical and electrophysiological study of 120 patients with different stages of an apoplectic coma. The authors studied the gas content, acid-base balance of the cranial blood inflow and outflow in the CSF and changes of EEG and EMG. It was shown that there were different levels of CNS lesions (cortico-subcortical, meso-diencephalic, meso-pontile and bulbar level) which corresponded to certain correlations between clinical, biochemical, EEG and EMG findings. The authors describe a classificational scheme of 4 stages of an apopletic coma. Special significance in the pathogenesis of a coma along with structural lesions in the different areas of the CNS is attached to metabolic disturbances and extracerebral factors (respiratory and circulatory disorders).", "PMID": 973512}
{"id": "pubmed23n0032_9315", "title": "[Psychopathologic symptomatology and its prognostic value in the acute period of right hemisphere strokes].", "content": "In order to evaluate the possible psychopathological symptomatology the authors analyzed the results of a clinico-dynamical observation of 176 patients in the acute period of a stroke. Among 128 patients with the right hemisphere localization of the focus (the main group) in 60 cases (47%) there were revealed psychopathological symptoms whereas in the control group (48 cases) with left hemispheric foci only 2 patients (4.2%) such disturbances were noted. The main expressions of psychopathological syndromes in patients with lesions of the right hemisphere were anosognosia, certain emotional reactions in the form of euphoria, a drop in purposeful activity, motor and mental aspontanity and specific confabulatory disorders. The qualitative and quantitative specificity of psychopathological syndromes in focal vascular lesions of the right hemisphere is supplementary material for further deep studies of the functional asymmetry of the brain hemispheres.", "contents": "[Psychopathologic symptomatology and its prognostic value in the acute period of right hemisphere strokes]. In order to evaluate the possible psychopathological symptomatology the authors analyzed the results of a clinico-dynamical observation of 176 patients in the acute period of a stroke. Among 128 patients with the right hemisphere localization of the focus (the main group) in 60 cases (47%) there were revealed psychopathological symptoms whereas in the control group (48 cases) with left hemispheric foci only 2 patients (4.2%) such disturbances were noted. The main expressions of psychopathological syndromes in patients with lesions of the right hemisphere were anosognosia, certain emotional reactions in the form of euphoria, a drop in purposeful activity, motor and mental aspontanity and specific confabulatory disorders. The qualitative and quantitative specificity of psychopathological syndromes in focal vascular lesions of the right hemisphere is supplementary material for further deep studies of the functional asymmetry of the brain hemispheres.", "PMID": 973513}
{"id": "pubmed23n0032_9316", "title": "[Ambulatory treatment with anticoagulents in the prevention of cerebral ischemia].", "content": "The degree of effectiveness of preventive therapy with anticoagulants, following brain infarctions and in the stage of transient disorderd brain ciruclation, was studied in comparison with the frequency of repeated ischemic disorders in 166 patients, and in a control group of 138 normals. During permanent administration of anticoagulants repeated ischemic episodes appeared less frequently than with other forms of medication. However, there was an increased amount of ischemic disorders after a discontinuation of the preparations. The most expressed signs of such conditions were in patients with repeated brain infarctions in a low arterial pressure and especially in the stage of transient disorders of brain circulation. In such cases the indications for the use of anticoagulants should be very limited.", "contents": "[Ambulatory treatment with anticoagulents in the prevention of cerebral ischemia]. The degree of effectiveness of preventive therapy with anticoagulants, following brain infarctions and in the stage of transient disorderd brain ciruclation, was studied in comparison with the frequency of repeated ischemic disorders in 166 patients, and in a control group of 138 normals. During permanent administration of anticoagulants repeated ischemic episodes appeared less frequently than with other forms of medication. However, there was an increased amount of ischemic disorders after a discontinuation of the preparations. The most expressed signs of such conditions were in patients with repeated brain infarctions in a low arterial pressure and especially in the stage of transient disorders of brain circulation. In such cases the indications for the use of anticoagulants should be very limited.", "PMID": 973514}
{"id": "pubmed23n0032_9317", "title": "[Rehabilitation of patients who have suffered a stroke with an aphasic syndrome].", "content": "A total of 70 patients with aphasic syndromes following a cerebral stroke were treated in a neurological department, a polyclinic and at home. The main diseases which led to the brain stroke were hypertensive disease and general atherosclerosis. The aphasic disturbances were due to hemorrhagic strokes in 19 patients and ischemic-in 51 patients. Training of speech rehabilitation began from the end of the first and the beginning of the second week after the stroke. The inclusion of logopedic lessons into the general complex of restorative therapy, a continuity in conditions of a hospital and a polyclinic, a possibility of a dynamical observation of the patients, dispensarization-all these measures according to the authors' opinion are important and necessary for rehabilitation of the patients who had strokes with the aphasic syndrome.", "contents": "[Rehabilitation of patients who have suffered a stroke with an aphasic syndrome]. A total of 70 patients with aphasic syndromes following a cerebral stroke were treated in a neurological department, a polyclinic and at home. The main diseases which led to the brain stroke were hypertensive disease and general atherosclerosis. The aphasic disturbances were due to hemorrhagic strokes in 19 patients and ischemic-in 51 patients. Training of speech rehabilitation began from the end of the first and the beginning of the second week after the stroke. The inclusion of logopedic lessons into the general complex of restorative therapy, a continuity in conditions of a hospital and a polyclinic, a possibility of a dynamical observation of the patients, dispensarization-all these measures according to the authors' opinion are important and necessary for rehabilitation of the patients who had strokes with the aphasic syndrome.", "PMID": 973515}
{"id": "pubmed23n0032_9318", "title": "[Lipoprotein metabolism disorders in hypertension with a cerebral syndrome and their diagnostic value for medico-occupational expertise].", "content": "On the basis of a profound clinico-biochemical study of 182 patients with hypertensive disease the authors established that the development of chronic insufficiency of cerebral circulation in this disorder is accompanied by quantitative and physico-chemical changes in the lipoprotein metabolism. These indices do not change significantly following short sessions of heparinotherpy and comprehensive treatment. Their diagnostical significance for medico-occupational examinations is discussed.", "contents": "[Lipoprotein metabolism disorders in hypertension with a cerebral syndrome and their diagnostic value for medico-occupational expertise]. On the basis of a profound clinico-biochemical study of 182 patients with hypertensive disease the authors established that the development of chronic insufficiency of cerebral circulation in this disorder is accompanied by quantitative and physico-chemical changes in the lipoprotein metabolism. These indices do not change significantly following short sessions of heparinotherpy and comprehensive treatment. Their diagnostical significance for medico-occupational examinations is discussed.", "PMID": 973516}
{"id": "pubmed23n0032_9319", "title": "[Electroencephalographic characteristics of cerebral atherosclerosis in patients of different ages].", "content": "On the basis of clinical and EEG studies of 500 patients from the age of 45-105 with diffuse cerebral atherosclerosis the author comes to the following conclusion. With an intensification of atherosclerosis there is an increase of alpha-rhythm disorganization, a slowing down of the frequency, a drop in the amplitude and index of alpha-rhythms, a decrease in the electrical brain reactivity with an increase of delta-and theta-waves. It is shown that the EEG changes are to a greater extent determined by the severity of cerebral atherosclerosis than by age factors. Normal variants of the EEG in patients with expressed atherosclerosis is conditioned by the compensatory intactness of the cortico-subcortical systems generating these rhythms.", "contents": "[Electroencephalographic characteristics of cerebral atherosclerosis in patients of different ages]. On the basis of clinical and EEG studies of 500 patients from the age of 45-105 with diffuse cerebral atherosclerosis the author comes to the following conclusion. With an intensification of atherosclerosis there is an increase of alpha-rhythm disorganization, a slowing down of the frequency, a drop in the amplitude and index of alpha-rhythms, a decrease in the electrical brain reactivity with an increase of delta-and theta-waves. It is shown that the EEG changes are to a greater extent determined by the severity of cerebral atherosclerosis than by age factors. Normal variants of the EEG in patients with expressed atherosclerosis is conditioned by the compensatory intactness of the cortico-subcortical systems generating these rhythms.", "PMID": 973518}
{"id": "pubmed23n0032_9320", "title": "[Age and features of adrenal cortical function in cerebral circulatory disorders].", "content": "The indices of glucocorticoid and androgen activity in the adrenal cortex (the level of circulating eosinophils, the daily urine excretion of 17 OCS and 17 CS) and the functional reserve after an ACTH loading was studied in 2 groups of patients with disorders of cerebral circulation under 45 years of age (247 cases) and over 55 (234 cases). In the acute period of a cerebral stroke there was an increase of the glucocorticoid activity which was more definitely expressed in the younger group and a certain inhibition of the androgen function seen mainly in the older group. In transient disorders of cerebral circulation significant age difference in the functioning of the adrenal cortex was not established. In the period following a stroke the established and potential reserves of the adrenocortical glands were distinctly decreased in patients above 55 years and practically unchanged in the younger group.", "contents": "[Age and features of adrenal cortical function in cerebral circulatory disorders]. The indices of glucocorticoid and androgen activity in the adrenal cortex (the level of circulating eosinophils, the daily urine excretion of 17 OCS and 17 CS) and the functional reserve after an ACTH loading was studied in 2 groups of patients with disorders of cerebral circulation under 45 years of age (247 cases) and over 55 (234 cases). In the acute period of a cerebral stroke there was an increase of the glucocorticoid activity which was more definitely expressed in the younger group and a certain inhibition of the androgen function seen mainly in the older group. In transient disorders of cerebral circulation significant age difference in the functioning of the adrenal cortex was not established. In the period following a stroke the established and potential reserves of the adrenocortical glands were distinctly decreased in patients above 55 years and practically unchanged in the younger group.", "PMID": 973517}
{"id": "pubmed23n0032_9321", "title": "[Cerebral and general hemodynamics in elderly patients with cerebral circulatory disorders].", "content": "The report is concerned with clinico-electrophysiological study of 112 patients of middle and old age with disorders of brain circulation. The obtained results depicting a cardio-vascular insufficiency were mainly seen in patients over 70 years of age. Changes of the heart activity negatively influenced the state of brain circulation. In the pathogenesis of cerebro-vascular changes especially in elderly and senile patients of special importance is cardiovascular insufficiency.", "contents": "[Cerebral and general hemodynamics in elderly patients with cerebral circulatory disorders]. The report is concerned with clinico-electrophysiological study of 112 patients of middle and old age with disorders of brain circulation. The obtained results depicting a cardio-vascular insufficiency were mainly seen in patients over 70 years of age. Changes of the heart activity negatively influenced the state of brain circulation. In the pathogenesis of cerebro-vascular changes especially in elderly and senile patients of special importance is cardiovascular insufficiency.", "PMID": 973519}
{"id": "pubmed23n0032_9322", "title": "[Effect of papaverine on cerebrospinal fluid and blood acid-base balance in patients with vascular diseases of the brain].", "content": "The authors studied the acid-base balance of the CSF, arterial and venous blood in patients with disorders of cerebral circulation by Astrup's method. An analysis of the obtained results detected a certain correlation between the dynamics of clinical signs and changes in the concentration of H ions in the CSF. THese indices may point to connections between the cerebral circulation and changes in the concentration of H ions in the CSF under the influence of papaverine.", "contents": "[Effect of papaverine on cerebrospinal fluid and blood acid-base balance in patients with vascular diseases of the brain]. The authors studied the acid-base balance of the CSF, arterial and venous blood in patients with disorders of cerebral circulation by Astrup's method. An analysis of the obtained results detected a certain correlation between the dynamics of clinical signs and changes in the concentration of H ions in the CSF. THese indices may point to connections between the cerebral circulation and changes in the concentration of H ions in the CSF under the influence of papaverine.", "PMID": 973520}
{"id": "pubmed23n0032_9323", "title": "[Efficacy of transecting the anterior scalene muscle in the scalenus anticus syndrome (syndrome of the scalene muscle)].", "content": "The authors observed 111 cases with the anterior scalenus syndrome. Ninety-three patients underwent surgical treatment. Acknowledging the predominant role of the anterior scalenus muscle in the pathogenesis of this disorder the authors performed scalenotomia. In the group of patients with a noncomplicated development of the disease (48 cases) the operation appeared to be effective in 93.7% of the cases, while in vascular complications (45 cases) the effectiveness of the operation was significantly reduced. A positive result in these cases was attained only in 51.1%. On the basis of these data the authors come to the conclusion that scalenotomia performed in the initial, noncomplicated stages of the disease is a good prophylactic measure for severe complications in the magistral vessels and is an effective method of treatment of the scalenus-anticus syndrome.", "contents": "[Efficacy of transecting the anterior scalene muscle in the scalenus anticus syndrome (syndrome of the scalene muscle)]. The authors observed 111 cases with the anterior scalenus syndrome. Ninety-three patients underwent surgical treatment. Acknowledging the predominant role of the anterior scalenus muscle in the pathogenesis of this disorder the authors performed scalenotomia. In the group of patients with a noncomplicated development of the disease (48 cases) the operation appeared to be effective in 93.7% of the cases, while in vascular complications (45 cases) the effectiveness of the operation was significantly reduced. A positive result in these cases was attained only in 51.1%. On the basis of these data the authors come to the conclusion that scalenotomia performed in the initial, noncomplicated stages of the disease is a good prophylactic measure for severe complications in the magistral vessels and is an effective method of treatment of the scalenus-anticus syndrome.", "PMID": 973521}
{"id": "pubmed23n0032_9324", "title": "[Confabulation in atrophic and vascular disease of old age (clinico-psychopathologic findings)].", "content": "The paper is concerned with a study of confabulation seen in 95 patients. A gradual development of the confabulatory syndrome (from mnemonic confabulations to ecmnestic) was seen in senile dementia (5 cases) and in its combination with vascular atherosclerosis (61 cases). An acute development of confabulations (with a prevalence of mnemonic false reminiscence over ecmnestic) was seen in cerebral atherosclerosis (9 cases). Rudimentary ecmnestic confabulations, without mnemonic were seen in Alzheimer's disease (20 cases). The author established a certain correlation between ecmnestic confabulations and disturbances of fixation, between mnemonic confabulations and disorders of selective reproductivity, between their expressiveness and emotional liveliness and the degree of actualization of the past events. In a complacent-euphoric and anxiety affect the confabulations were spontaneous and were accompanied by psuedoactivity. In depressive states they were fragmentary and were detected only in response to questions. Aphatic disorders and the severity of dementia influence the mnestic confabulations making them poorer and incomplete.", "contents": "[Confabulation in atrophic and vascular disease of old age (clinico-psychopathologic findings)]. The paper is concerned with a study of confabulation seen in 95 patients. A gradual development of the confabulatory syndrome (from mnemonic confabulations to ecmnestic) was seen in senile dementia (5 cases) and in its combination with vascular atherosclerosis (61 cases). An acute development of confabulations (with a prevalence of mnemonic false reminiscence over ecmnestic) was seen in cerebral atherosclerosis (9 cases). Rudimentary ecmnestic confabulations, without mnemonic were seen in Alzheimer's disease (20 cases). The author established a certain correlation between ecmnestic confabulations and disturbances of fixation, between mnemonic confabulations and disorders of selective reproductivity, between their expressiveness and emotional liveliness and the degree of actualization of the past events. In a complacent-euphoric and anxiety affect the confabulations were spontaneous and were accompanied by psuedoactivity. In depressive states they were fragmentary and were detected only in response to questions. Aphatic disorders and the severity of dementia influence the mnestic confabulations making them poorer and incomplete.", "PMID": 973523}
{"id": "pubmed23n0032_9325", "title": "[Morphologic basis of psychoses associated with vascular and senile pathology].", "content": "The paper deals with a study of 100 patients diagnosed as combined senile and vascular psychoses. It is marked that in classical cases of such combinations the disorder has 3 stages. On the first stage there is a predominance of vascular pathology. After 60 years of age (the second stage) the senile sumptomatology disguises many symptoms of vascular lesions. The third stage is characterized by a vascular catastrophe. In many cases of combined psychoses there may be complications of other somatic pathology and mainly of lung and liver disorders.", "contents": "[Morphologic basis of psychoses associated with vascular and senile pathology]. The paper deals with a study of 100 patients diagnosed as combined senile and vascular psychoses. It is marked that in classical cases of such combinations the disorder has 3 stages. On the first stage there is a predominance of vascular pathology. After 60 years of age (the second stage) the senile sumptomatology disguises many symptoms of vascular lesions. The third stage is characterized by a vascular catastrophe. In many cases of combined psychoses there may be complications of other somatic pathology and mainly of lung and liver disorders.", "PMID": 973524}
{"id": "pubmed23n0032_9326", "title": "[Korsakoff syndrome of traumatic genesis (development, structure, course)].", "content": "The conducted study of the development and structure of the Korsakoff syndrome of a traumatic origin shows that the syndrome has its own regularities of development. An analysis of its course demonstrates an expressed development of sequalae due to brain injuries. The evaluation of the Korsakoff syndrome and its components permits to predict at the initial phase of its appearance the clinical outcome and consequently the tactics of treatment and possible social readaptation.", "contents": "[Korsakoff syndrome of traumatic genesis (development, structure, course)]. The conducted study of the development and structure of the Korsakoff syndrome of a traumatic origin shows that the syndrome has its own regularities of development. An analysis of its course demonstrates an expressed development of sequalae due to brain injuries. The evaluation of the Korsakoff syndrome and its components permits to predict at the initial phase of its appearance the clinical outcome and consequently the tactics of treatment and possible social readaptation.", "PMID": 973525}
{"id": "pubmed23n0032_9327", "title": "[Dynamics of the dementia syndrome].", "content": "The author discusses some modern problems concerning the clinical pictures of dementia which have a certain theoretical and practical significance. The paper contains some results related to the dynamics of syndromes in acquired dementia and of the most frequently seen variants in the development of such conditions. The possibility of diagnosing dementia corresponding to the phases of mental disorders are discussed. The dynamics  of dementia syndromes are considered from the point of view of their nosological specificity.", "contents": "[Dynamics of the dementia syndrome]. The author discusses some modern problems concerning the clinical pictures of dementia which have a certain theoretical and practical significance. The paper contains some results related to the dynamics of syndromes in acquired dementia and of the most frequently seen variants in the development of such conditions. The possibility of diagnosing dementia corresponding to the phases of mental disorders are discussed. The dynamics  of dementia syndromes are considered from the point of view of their nosological specificity.", "PMID": 973526}
{"id": "pubmed23n0032_9328", "title": "[Mental disorders in meningiomas of the olfactory fossa].", "content": "In 19 patients with meningiomas of the olfactory fossa the author established a certain development of the psychopathological symptomatology. First of all asthenia ensues, eventually a complacent mood with an underestimation of the severity of the disease and an emotional crudeness is seen. These disorders are gradually substituted by euphoric dementia with a tendency towards foolish jokes, anosognosia of the disease, severe intellectual disorders and a desintegration of the \"personality nuclei\" with desinhibited drives. The intellectual disorders are expressed not in formal disturbances (logic operations), but in insufficient goal-directness and uncritical thinking.", "contents": "[Mental disorders in meningiomas of the olfactory fossa]. In 19 patients with meningiomas of the olfactory fossa the author established a certain development of the psychopathological symptomatology. First of all asthenia ensues, eventually a complacent mood with an underestimation of the severity of the disease and an emotional crudeness is seen. These disorders are gradually substituted by euphoric dementia with a tendency towards foolish jokes, anosognosia of the disease, severe intellectual disorders and a desintegration of the \"personality nuclei\" with desinhibited drives. The intellectual disorders are expressed not in formal disturbances (logic operations), but in insufficient goal-directness and uncritical thinking.", "PMID": 973527}
{"id": "pubmed23n0032_9329", "title": "[Clinical picture and dynamics of psychopathologic disorders in subacute leuko-and panencephalitis].", "content": "The report contains the results of clinico-dynamic studies of mental disorders in patients with Schilder's leukoencephalitis and subacute sclerosing panencephalitis after van-Bogart. The authors come to the conclusion that only parallel comparisons of the psychopathological symptomatology with focal neurological signs and a simultaneous study of the EEG and other paraclinical indices may facilitate the differential diagnosis of encephalitis.", "contents": "[Clinical picture and dynamics of psychopathologic disorders in subacute leuko-and panencephalitis]. The report contains the results of clinico-dynamic studies of mental disorders in patients with Schilder's leukoencephalitis and subacute sclerosing panencephalitis after van-Bogart. The authors come to the conclusion that only parallel comparisons of the psychopathological symptomatology with focal neurological signs and a simultaneous study of the EEG and other paraclinical indices may facilitate the differential diagnosis of encephalitis.", "PMID": 973528}
{"id": "pubmed23n0032_9330", "title": "[Direction of the clinical pathomorphosis of alcoholic delirium].", "content": "An examination of 348 patients with alcoholic delirium permitted to detect the main directions in the evolutional variability of the clinical picture. The authors distinguished 3 types in the development of a delirium: short-term with a complete and incomplete (abortive) variant; typical delirium with mono-and polymorphic structure of the syndrome; a protracted development in the form of a severe psychoses of a complex structure and a sluggish protracted variant with an obliviated symptomatology.", "contents": "[Direction of the clinical pathomorphosis of alcoholic delirium]. An examination of 348 patients with alcoholic delirium permitted to detect the main directions in the evolutional variability of the clinical picture. The authors distinguished 3 types in the development of a delirium: short-term with a complete and incomplete (abortive) variant; typical delirium with mono-and polymorphic structure of the syndrome; a protracted development in the form of a severe psychoses of a complex structure and a sluggish protracted variant with an obliviated symptomatology.", "PMID": 973529}
{"id": "pubmed23n0032_9331", "title": "[Clinical characteristics of psychopathologic changes induced by medicinal anti-epileptic therapy].", "content": "On the basis of 48 clinical observations the authors describe some psychopathological changes due to certain anticonvulsive drugs used in the treatment of epilepsy (phenobarbital with diphenin or hexamindin, phenobarbital with finlepsin and bensodiazepine). The report contains an analysis of the causes leading to complications: a relative and absolute overdosage, a negative and positive interference of drugs.", "contents": "[Clinical characteristics of psychopathologic changes induced by medicinal anti-epileptic therapy]. On the basis of 48 clinical observations the authors describe some psychopathological changes due to certain anticonvulsive drugs used in the treatment of epilepsy (phenobarbital with diphenin or hexamindin, phenobarbital with finlepsin and bensodiazepine). The report contains an analysis of the causes leading to complications: a relative and absolute overdosage, a negative and positive interference of drugs.", "PMID": 973530}
{"id": "pubmed23n0032_9332", "title": "[Interaction of psychotropic and anticonvulsive drugs].", "content": "Experiments on rats of the Wistar strain has  shown that preliminary repeated introduction of phenobarbitals and chlordiazepoxide weakens the pharmacological effect of medications, increases the liver weight and the protein content, RNA, R-450 cytochrome, accelerates the oxidation of substrates, but to a higher degree of hexobarbital. Aminazine and mellipramine do not significantly influence these indices changing, however, the affinity of R-450 cytochrome to different substrates and the speed of their oxidation.", "contents": "[Interaction of psychotropic and anticonvulsive drugs]. Experiments on rats of the Wistar strain has  shown that preliminary repeated introduction of phenobarbitals and chlordiazepoxide weakens the pharmacological effect of medications, increases the liver weight and the protein content, RNA, R-450 cytochrome, accelerates the oxidation of substrates, but to a higher degree of hexobarbital. Aminazine and mellipramine do not significantly influence these indices changing, however, the affinity of R-450 cytochrome to different substrates and the speed of their oxidation.", "PMID": 973531}
{"id": "pubmed23n0032_9333", "title": "Studies on the oogonial cycle in Puntius ticto (Ham.) I. Formation of primary oocytes.", "content": "Histologically, the ovary of Puntius ticto (Ham.) is covered with three layers, namely peritoneum, tunica albuginea and germinal epithelium. The last two give rise to the ovigerous nests in the ovocoel. The new crop of oocytes is either derived from the germ cells present in the ovigerous nests, or from the epithelium of the outer wall of the ovary, or from the epithelium of the ovigerous folds.", "contents": "Studies on the oogonial cycle in Puntius ticto (Ham.) I. Formation of primary oocytes. Histologically, the ovary of Puntius ticto (Ham.) is covered with three layers, namely peritoneum, tunica albuginea and germinal epithelium. The last two give rise to the ovigerous nests in the ovocoel. The new crop of oocytes is either derived from the germ cells present in the ovigerous nests, or from the epithelium of the outer wall of the ovary, or from the epithelium of the ovigerous folds.", "PMID": 973535}
{"id": "pubmed23n0032_9334", "title": "The arterial supply of the urinary bladder.", "content": "(1) The urachal artery has a primary origin from the umbilical artery itself, and is at first bilateral. (2) The arterial pattern in newborn infants is not identical to that of adults. Obliteration of the umbilical artery in older infants carries with it obliteration of some of its branches, especially at their origin from its distal part, with a resulting change in arterial pattern. (3) The middle vesicle artery is a fairly constant branch of the umbilical artery, supplying the bladder neck and the front of the bladder above the neck. (4) The urachal and middle vesicle arteries, when unilateral, occur with equal frequency on both sides. (5) The anastomosis between superior vesical and inferior episgastric arteries was confirmed in the subperitoneal tissues, but was very rare in the wall of the bladder, occurring only when an abnormal obturator artery was the source of a superior vesical branch. (6) The constancy of the vesiculo-deferential artery is confirmed.", "contents": "The arterial supply of the urinary bladder. (1) The urachal artery has a primary origin from the umbilical artery itself, and is at first bilateral. (2) The arterial pattern in newborn infants is not identical to that of adults. Obliteration of the umbilical artery in older infants carries with it obliteration of some of its branches, especially at their origin from its distal part, with a resulting change in arterial pattern. (3) The middle vesicle artery is a fairly constant branch of the umbilical artery, supplying the bladder neck and the front of the bladder above the neck. (4) The urachal and middle vesicle arteries, when unilateral, occur with equal frequency on both sides. (5) The anastomosis between superior vesical and inferior episgastric arteries was confirmed in the subperitoneal tissues, but was very rare in the wall of the bladder, occurring only when an abnormal obturator artery was the source of a superior vesical branch. (6) The constancy of the vesiculo-deferential artery is confirmed.", "PMID": 973536}
{"id": "pubmed23n0032_9335", "title": "Cholinergic innervation of vessels of the base of the brain.", "content": "Distribution and activity of acetylcholinesterase in the intramural nerve apparatus of vessels of the base of the bran have been studied with the histochemical method of Koelle-Gomori. On the whole, 268 arteries have been examined. The activity of the ferment is detected in the effector nerve fibers in the wall of all cerebral vessels constituting the system of the circle of Willis. The periarterial nerve apparatus of cerebral arteries of the anterior and posterior regions of the circle of Willis consists of two cholinergic plexuses - superficial and deep plexus forming a multilayered neural cover. The terminal parts of cholinergic nerve fibres at the border with the muscular coat show a granular structure. Moreover, nerve cells rich in active ferment are found in the cholinergic plexuses. Difference in the character of cholinergic innervation of vessels of the internal carotid and the vertebrobasilar region has been shown. The obtained data indicate that the vessels of the circle of Willis possess a reliable cholinergic system of nerve regulation which along with other systems, such as that of adrenergic nerve fibers, maintains the homeostasis of the cerebral blood flow.", "contents": "Cholinergic innervation of vessels of the base of the brain. Distribution and activity of acetylcholinesterase in the intramural nerve apparatus of vessels of the base of the bran have been studied with the histochemical method of Koelle-Gomori. On the whole, 268 arteries have been examined. The activity of the ferment is detected in the effector nerve fibers in the wall of all cerebral vessels constituting the system of the circle of Willis. The periarterial nerve apparatus of cerebral arteries of the anterior and posterior regions of the circle of Willis consists of two cholinergic plexuses - superficial and deep plexus forming a multilayered neural cover. The terminal parts of cholinergic nerve fibres at the border with the muscular coat show a granular structure. Moreover, nerve cells rich in active ferment are found in the cholinergic plexuses. Difference in the character of cholinergic innervation of vessels of the internal carotid and the vertebrobasilar region has been shown. The obtained data indicate that the vessels of the circle of Willis possess a reliable cholinergic system of nerve regulation which along with other systems, such as that of adrenergic nerve fibers, maintains the homeostasis of the cerebral blood flow.", "PMID": 973537}
{"id": "pubmed23n0032_9336", "title": "Wool follicle characteristics in the Awassi fat-tailed sheep.", "content": "In Awassi sheep, the total number of wool follicles per square millimetre was affected significantly by age, sex, and type of birth. The density of the primary follicles was only significantly affected by age and type of birth. The secondary follicles to primary follicles (S/P) ratio values were 2.0 at birth, 4.0 at 2 months and 4.2 at 6 months of age, in Awassi sheep. The males exceeded the females, in most ages of the experiment. The single lambs surpassed the twins in the S/P ratio values. The effect of age and type of birth on the S/P ratio was highly significant. The external diameter of wool follicles increased with higher values at 2 months of age than afterwards, till 6 months of age. The contrary was the case in the internal diameters of wool follicles. The increase was higher in the primary than in the secondary follicles, during the period of the study. The males surpassed, in general, the females in the values of the external and internal diameters of the primary and secondary follicles. Single lambs showed higher values than twins in the external and internal diameters of primary and secondary follicles in most ages of the experiment. Age and type of birth affected significantly the diameters of follicles, while sex effect was only highly significant on the external diameter of the secondary follicles. The length of the primary and secondary follicles and the thickness of skin increased significantly, as the animals grew older. Sex showed no significant effect on the length of follicles, while it affected significantly the thickness of the skin. Single lambs showed higher significant values than twins, in length of follicles and skin thickness. The bulbs of each of the primary and secondary follicles increased significantly, as the animals grew older. Sex had no significant effect on the bulb diameters of the two types of follicles. The single lambs surpassed the twins in the values for follicle bulb diameter, in most ages of the experiment. The effect of the type of birth on the bulb diameter of the two types of follicles was highly significant.", "contents": "Wool follicle characteristics in the Awassi fat-tailed sheep. In Awassi sheep, the total number of wool follicles per square millimetre was affected significantly by age, sex, and type of birth. The density of the primary follicles was only significantly affected by age and type of birth. The secondary follicles to primary follicles (S/P) ratio values were 2.0 at birth, 4.0 at 2 months and 4.2 at 6 months of age, in Awassi sheep. The males exceeded the females, in most ages of the experiment. The single lambs surpassed the twins in the S/P ratio values. The effect of age and type of birth on the S/P ratio was highly significant. The external diameter of wool follicles increased with higher values at 2 months of age than afterwards, till 6 months of age. The contrary was the case in the internal diameters of wool follicles. The increase was higher in the primary than in the secondary follicles, during the period of the study. The males surpassed, in general, the females in the values of the external and internal diameters of the primary and secondary follicles. Single lambs showed higher values than twins in the external and internal diameters of primary and secondary follicles in most ages of the experiment. Age and type of birth affected significantly the diameters of follicles, while sex effect was only highly significant on the external diameter of the secondary follicles. The length of the primary and secondary follicles and the thickness of skin increased significantly, as the animals grew older. Sex showed no significant effect on the length of follicles, while it affected significantly the thickness of the skin. Single lambs showed higher significant values than twins, in length of follicles and skin thickness. The bulbs of each of the primary and secondary follicles increased significantly, as the animals grew older. Sex had no significant effect on the bulb diameters of the two types of follicles. The single lambs surpassed the twins in the values for follicle bulb diameter, in most ages of the experiment. The effect of the type of birth on the bulb diameter of the two types of follicles was highly significant.", "PMID": 973539}
{"id": "pubmed23n0032_9337", "title": "Observations on the effects of alpha-chlorhydrin on the testes and pituitary gonadotrophs of gerbil (Meriones hurrianae) and rat.", "content": "(1) Lower doses of alpha-chlorhydrin caused high selectivity of lesions in the seminiferous tubule of rat and gerbil. (2) The seminiferous epithelium became systematically depleted of spermatogonia, spermatocytes, spermatids and finally spermatozoa in this sequence. (3) The suppression of RNA concentration in the testes and seminal vesicle is conspicuous. (4) The growth of androgen-dependent organs, i.e. seminal vesicles, ventral prostate, epididymes and perineal complex, is suppressed. (5) These effects are reversible. Repopulation of testis tubules occurs followed by a 40-day recovery period in rat and gerbil. (6) alpha-Chlorhydrin administration brings about hypertrophy of pituitary gonadotrophs which is also reflected in the increased basophilic cell percentage (control: 15.5% alpha-chlorhydrin: 21.5%; p less than 0.01).", "contents": "Observations on the effects of alpha-chlorhydrin on the testes and pituitary gonadotrophs of gerbil (Meriones hurrianae) and rat. (1) Lower doses of alpha-chlorhydrin caused high selectivity of lesions in the seminiferous tubule of rat and gerbil. (2) The seminiferous epithelium became systematically depleted of spermatogonia, spermatocytes, spermatids and finally spermatozoa in this sequence. (3) The suppression of RNA concentration in the testes and seminal vesicle is conspicuous. (4) The growth of androgen-dependent organs, i.e. seminal vesicles, ventral prostate, epididymes and perineal complex, is suppressed. (5) These effects are reversible. Repopulation of testis tubules occurs followed by a 40-day recovery period in rat and gerbil. (6) alpha-Chlorhydrin administration brings about hypertrophy of pituitary gonadotrophs which is also reflected in the increased basophilic cell percentage (control: 15.5% alpha-chlorhydrin: 21.5%; p less than 0.01).", "PMID": 973538}
{"id": "pubmed23n0032_9338", "title": "The action of the hind limb musculature of the dog in walking.", "content": "This study was performed by means of cinephotography and electromyography. The results were correlated with earlier investigations concerning the forces exerted by the pads of walking dogs. A concept about the action of individual muscles of the hind limb during a stride was formed. The principal conclusions are: (1) The hamstrings are divisible into a cranial and a caudal group, the first consisting of the cranial parts of the M. biceps femoris and M. semimembranosus, the second of the caudal parts of these muscles, together with the M. semitendinosus. The chief function of both groups is to extend the hip; the caudal group also flexes the stifle. (2) Activity is present in the greatest number of muscle s during the change in the sense of movement of the limb. (3) During the last stages of the stance phase the number of muscles showing electrical activity diminishes, while the force exerted by the pads in a horizontal direction increases. Non-muscular forces, e.g. inertia, play an important role during movement.", "contents": "The action of the hind limb musculature of the dog in walking. This study was performed by means of cinephotography and electromyography. The results were correlated with earlier investigations concerning the forces exerted by the pads of walking dogs. A concept about the action of individual muscles of the hind limb during a stride was formed. The principal conclusions are: (1) The hamstrings are divisible into a cranial and a caudal group, the first consisting of the cranial parts of the M. biceps femoris and M. semimembranosus, the second of the caudal parts of these muscles, together with the M. semitendinosus. The chief function of both groups is to extend the hip; the caudal group also flexes the stifle. (2) Activity is present in the greatest number of muscle s during the change in the sense of movement of the limb. (3) During the last stages of the stance phase the number of muscles showing electrical activity diminishes, while the force exerted by the pads in a horizontal direction increases. Non-muscular forces, e.g. inertia, play an important role during movement.", "PMID": 973540}
{"id": "pubmed23n0032_9339", "title": "Adaptive differences in the body proportions of large felids.", "content": "Body proportions of eight species of large felids were studied and the relationship between their morphology, habitat structure and habitat utilization was analyzed. Both Panthera onca and Neofelis nebulosa are exclusive forest dwellers (found exclusively in high densly structured habitats). These felids have the relatively shortest anterior limbs and lumbar spine. In addition, P. onca has the relatively shortest posterior limbs of the large felids, while the posterior limbs of N. nebulosa are relatively long when compared with those of P. onca which seems to be an adaptation for jumping. All large fields use the anterior limbs to make primary contact with their prey. In addition, the prey taken by the forest fields can exceed their own body weight. On the other hand, the cheetah is found in low structured habitats (savanna), and it has the relatively longest limbs and lumbarspine of the large felids. But the cheetah's prey usually weigh less than its own body weight. The cheetah also employs a 'rapid pursuit phase' in hunting and is capable of overtaking its prey. The lion, however, was found to have the body proportions of a forest felid, yet it also resides in low structured habitats. It is postulated that the utilization of large prey by the lion limits the possibilities for morphological adaptations for speed. To increase hunting efficiency, reproductive success, and territorial defense on the plains, lions form prides.", "contents": "Adaptive differences in the body proportions of large felids. Body proportions of eight species of large felids were studied and the relationship between their morphology, habitat structure and habitat utilization was analyzed. Both Panthera onca and Neofelis nebulosa are exclusive forest dwellers (found exclusively in high densly structured habitats). These felids have the relatively shortest anterior limbs and lumbar spine. In addition, P. onca has the relatively shortest posterior limbs of the large felids, while the posterior limbs of N. nebulosa are relatively long when compared with those of P. onca which seems to be an adaptation for jumping. All large fields use the anterior limbs to make primary contact with their prey. In addition, the prey taken by the forest fields can exceed their own body weight. On the other hand, the cheetah is found in low structured habitats (savanna), and it has the relatively longest limbs and lumbarspine of the large felids. But the cheetah's prey usually weigh less than its own body weight. The cheetah also employs a 'rapid pursuit phase' in hunting and is capable of overtaking its prey. The lion, however, was found to have the body proportions of a forest felid, yet it also resides in low structured habitats. It is postulated that the utilization of large prey by the lion limits the possibilities for morphological adaptations for speed. To increase hunting efficiency, reproductive success, and territorial defense on the plains, lions form prides.", "PMID": 973541}
{"id": "pubmed23n0032_9340", "title": "Experimentally induced osteoarthrosis in the temporomandibular joint of the mouse.", "content": "(1) 6-week-old ICR mice were injected daily with pharmacologic doses of triamcinolone diacetate (1.5 mg/kg body weight) for 8 consecutive weeks. (2) The temporomandibular joint served as a model for histological and histochemical examinations concerning the effect of glucocorticoid hormone upon bone and cartilage. (3) Histochemical reactions indicated a large number of lipid droplets within the peripheral vasculature. (4) Histological examinations revealed pathologic features, indicative of primary osteoarthrosis.", "contents": "Experimentally induced osteoarthrosis in the temporomandibular joint of the mouse. (1) 6-week-old ICR mice were injected daily with pharmacologic doses of triamcinolone diacetate (1.5 mg/kg body weight) for 8 consecutive weeks. (2) The temporomandibular joint served as a model for histological and histochemical examinations concerning the effect of glucocorticoid hormone upon bone and cartilage. (3) Histochemical reactions indicated a large number of lipid droplets within the peripheral vasculature. (4) Histological examinations revealed pathologic features, indicative of primary osteoarthrosis.", "PMID": 973542}
{"id": "pubmed23n0032_9341", "title": "[Aneurysm at the bifurcation of the internal carotid artery (author's transl)].", "content": "The authors analyse 14 cases of aneurysm occurring at the point of bifurcation of the internal carotid artery into the middle and anterior cerebral arteries. Distinguishing features of this aneurysm are often its fairly large size and extensive implantation on the artery. Clinical symptoms are not specific but various neurological signs are frequent. The bifurcation of the carotid artery is not always readily visible; oblique views and angiotomography are often necessary. Owing to the usual severity of the natural evolution of this type of aneurysm (death in six out of eight cases), surgical treatment is advisable. Ligation of the carotid artery is disadvised because of the unpredictable nature of its results (further fatal haemorrhage in one out of three cases).", "contents": "[Aneurysm at the bifurcation of the internal carotid artery (author's transl)]. The authors analyse 14 cases of aneurysm occurring at the point of bifurcation of the internal carotid artery into the middle and anterior cerebral arteries. Distinguishing features of this aneurysm are often its fairly large size and extensive implantation on the artery. Clinical symptoms are not specific but various neurological signs are frequent. The bifurcation of the carotid artery is not always readily visible; oblique views and angiotomography are often necessary. Owing to the usual severity of the natural evolution of this type of aneurysm (death in six out of eight cases), surgical treatment is advisable. Ligation of the carotid artery is disadvised because of the unpredictable nature of its results (further fatal haemorrhage in one out of three cases).", "PMID": 973543}
{"id": "pubmed23n0032_9342", "title": "The arterial angioarchitecture in lacunar state.", "content": "The statistical data, obtained in seventy five brains with lacunar state, show that the lenticular nuclei and the periventricular arterial end-and borderzones are the main seats of the infarcts. In teen brains the relationship between the deep arterial angioarchitecture and the location of thirty lacunes is studied by means of the translucidation technique after filling the arterial system with a collo\u00eedal barium sulphate solution. The deep perforating arteries are poorly filled and show several narrowings and post-stenotic dilatations. In twenty-four lacunes, an occlusion of the responsible artery is found. In the six remaining lacunes, no occlusion of the main artery is observed but the side-branches are not demonstrated. The significance of these findings and the relationship between the location of the occlusion and the topography of the lacune are discussed.", "contents": "The arterial angioarchitecture in lacunar state. The statistical data, obtained in seventy five brains with lacunar state, show that the lenticular nuclei and the periventricular arterial end-and borderzones are the main seats of the infarcts. In teen brains the relationship between the deep arterial angioarchitecture and the location of thirty lacunes is studied by means of the translucidation technique after filling the arterial system with a collo\u00eedal barium sulphate solution. The deep perforating arteries are poorly filled and show several narrowings and post-stenotic dilatations. In twenty-four lacunes, an occlusion of the responsible artery is found. In the six remaining lacunes, no occlusion of the main artery is observed but the side-branches are not demonstrated. The significance of these findings and the relationship between the location of the occlusion and the topography of the lacune are discussed.", "PMID": 973544}
{"id": "pubmed23n0032_9343", "title": "Total 5-hydroxyindoles in blood related to migraine attacks.", "content": "The total 5-hydroxyindoles (5HI) in whole blood were measured in 20 migraine patients during spontaneous migraine attacks and in headache-free periods. A statistically significant fall in blood 5-HI was found during headache in 17 patients suffering from classical and common migraine. In one patient with complicated migraine no change was found, and in two patients, one with common migraine and one with migraine and associated symptoms, there was a rise in total blood 5-HI during migraine attacks. The results are compared with previous findings in this field, and it is suggested that during migraine attacks there might be a rise in the plasma 5-HI. The possibility of using the 5-HI fall during spontaneous migraine attacks as a simple test for the diagnosis of migraine is discussed.", "contents": "Total 5-hydroxyindoles in blood related to migraine attacks. The total 5-hydroxyindoles (5HI) in whole blood were measured in 20 migraine patients during spontaneous migraine attacks and in headache-free periods. A statistically significant fall in blood 5-HI was found during headache in 17 patients suffering from classical and common migraine. In one patient with complicated migraine no change was found, and in two patients, one with common migraine and one with migraine and associated symptoms, there was a rise in total blood 5-HI during migraine attacks. The results are compared with previous findings in this field, and it is suggested that during migraine attacks there might be a rise in the plasma 5-HI. The possibility of using the 5-HI fall during spontaneous migraine attacks as a simple test for the diagnosis of migraine is discussed.", "PMID": 973551}
{"id": "pubmed23n0032_9344", "title": "Periodic electroencephalographic compleses and subacute sclerosing panencephalitis. A case report and a survey of literature.", "content": "The authors report the case of a child with a presumable diagnosis of subacute sclerosing panencephalitis (SSPE). But pathological examination of the brain showed only a moderate neuronal loss in the cortical and subcortical grey matter. From this case and from the data in the literature it is concluded that the electroencephalogram in stage II of SSPE is characterized by the appearance of paroxysmal wave complexes which recur pseudo-periodically against a disorganized basic activity. The criteria for these complexes are not as strict and the periodicity is not as regular as formerly believed. Such a tracing is not pathognomonic of SSPE and may also occur-although rarely-in other brain diseases. The theory that the periodic discharges originate from a diffuse alteration of cortical and subcortical grey matter seems the most plausible.", "contents": "Periodic electroencephalographic compleses and subacute sclerosing panencephalitis. A case report and a survey of literature. The authors report the case of a child with a presumable diagnosis of subacute sclerosing panencephalitis (SSPE). But pathological examination of the brain showed only a moderate neuronal loss in the cortical and subcortical grey matter. From this case and from the data in the literature it is concluded that the electroencephalogram in stage II of SSPE is characterized by the appearance of paroxysmal wave complexes which recur pseudo-periodically against a disorganized basic activity. The criteria for these complexes are not as strict and the periodicity is not as regular as formerly believed. Such a tracing is not pathognomonic of SSPE and may also occur-although rarely-in other brain diseases. The theory that the periodic discharges originate from a diffuse alteration of cortical and subcortical grey matter seems the most plausible.", "PMID": 973545}
{"id": "pubmed23n0032_9345", "title": "Arginine esterase in cerebrospinal fluid and pro-arginine esterase in plasma from patients with migraine.", "content": "Estimates of prekallikrein levels in plasma specimens from patients with migraine and from healthy individuals were obtained by determining the benzoyl-arginine ethyl ester (BAEe) esterase activities developed on activation with kaolin, as suggested by Costerase level- 'n the patients and in the control material, and kinetic data provided no evidence of a difference in inhibitor levels. Only very low BAEe esterase activity was registered in samples of cerebrospinal fluid obtained from the patients and no significant difference between attacks and free intervals was detected. When citrated EDTA-treated plasma was activated with acetone-incubated normal plasma containing prekallikrein activator (factor XIIf), no significant difference in BAEe esterase activity was noticed between plasma from the patients and that from the control persons. When, however, citrated plasma without EDTA  was used, a significantly higher peak level of esterase activity was registered in the patient plasma. This observation might suggest the presence of a factor positioned between active factor XII and prekallikrein, and present in higher amounts in plasma from patients with migraine than in healthy individuals.", "contents": "Arginine esterase in cerebrospinal fluid and pro-arginine esterase in plasma from patients with migraine. Estimates of prekallikrein levels in plasma specimens from patients with migraine and from healthy individuals were obtained by determining the benzoyl-arginine ethyl ester (BAEe) esterase activities developed on activation with kaolin, as suggested by Costerase level- 'n the patients and in the control material, and kinetic data provided no evidence of a difference in inhibitor levels. Only very low BAEe esterase activity was registered in samples of cerebrospinal fluid obtained from the patients and no significant difference between attacks and free intervals was detected. When citrated EDTA-treated plasma was activated with acetone-incubated normal plasma containing prekallikrein activator (factor XIIf), no significant difference in BAEe esterase activity was noticed between plasma from the patients and that from the control persons. When, however, citrated plasma without EDTA  was used, a significantly higher peak level of esterase activity was registered in the patient plasma. This observation might suggest the presence of a factor positioned between active factor XII and prekallikrein, and present in higher amounts in plasma from patients with migraine than in healthy individuals.", "PMID": 973552}
{"id": "pubmed23n0032_9346", "title": "[Periodic discharges observed in a cerebral vascular accident (author's transl)].", "content": "The authors report a case of cerebral softening in which almost entirely unilateral periodic discharges were observed. They consisted of isolated high-voltage spiked waves predominating distinctly on the side of the lesion. The interval between spikes was always 2-3 seconds. The origin of these signs is discussed. The fact that they are not necessarily related to an infectious or metabolic disease is underlined.", "contents": "[Periodic discharges observed in a cerebral vascular accident (author's transl)]. The authors report a case of cerebral softening in which almost entirely unilateral periodic discharges were observed. They consisted of isolated high-voltage spiked waves predominating distinctly on the side of the lesion. The interval between spikes was always 2-3 seconds. The origin of these signs is discussed. The fact that they are not necessarily related to an infectious or metabolic disease is underlined.", "PMID": 973546}
{"id": "pubmed23n0032_9347", "title": "A prospective study of parapareses in western Sweden.", "content": "As a consequence of a WHO inquiry about possible complications to poliomyelitis immunization, a prospective incidence study was performed during 1971 in the western region of Sweden, in order to obtain data relevant to the inactivated vaccine used here. After rejection of surgical and secondary cases, the study consisted of 10 Guillain-Barr\u00e9 cases, three incipient MS cases, two infectious and one probably vascular case. By using a procedure of retrospective controls it was demonstrated that the efficiency of prospective case ascertainment is dependent on diagnosis. The incidence of de novo parapareses as defined was approximately 1.7/100,000/year of which 1.0/100,000/year was due to MS. CSF and virological investigations were performed early in prospective cases, in MS cases within 5 to 11 days after onset, and it was shown that no changes occurred in later CF titrations. Routine virological tests did not contribute to diagnosis in patients having paraparesis. No vaccine-associated cases of paraparesis occurred during the 1-year period of the study.", "contents": "A prospective study of parapareses in western Sweden. As a consequence of a WHO inquiry about possible complications to poliomyelitis immunization, a prospective incidence study was performed during 1971 in the western region of Sweden, in order to obtain data relevant to the inactivated vaccine used here. After rejection of surgical and secondary cases, the study consisted of 10 Guillain-Barr\u00e9 cases, three incipient MS cases, two infectious and one probably vascular case. By using a procedure of retrospective controls it was demonstrated that the efficiency of prospective case ascertainment is dependent on diagnosis. The incidence of de novo parapareses as defined was approximately 1.7/100,000/year of which 1.0/100,000/year was due to MS. CSF and virological investigations were performed early in prospective cases, in MS cases within 5 to 11 days after onset, and it was shown that no changes occurred in later CF titrations. Routine virological tests did not contribute to diagnosis in patients having paraparesis. No vaccine-associated cases of paraparesis occurred during the 1-year period of the study.", "PMID": 973553}
{"id": "pubmed23n0032_9348", "title": "[Programmed rehabilitation of a case of agraphia (author's transl)].", "content": "The authors describe a programmed method of rehabilitation suitable for patients with writing disorders resulting from cerebral lesions. The use of a visual display screen permits differential display and the immediate correction of errors. The application of the method is illustrated in the case of a patient with aphasic agraphia, and its general utility is briefly discussed in relation to the nature of various graphic disorders.", "contents": "[Programmed rehabilitation of a case of agraphia (author's transl)]. The authors describe a programmed method of rehabilitation suitable for patients with writing disorders resulting from cerebral lesions. The use of a visual display screen permits differential display and the immediate correction of errors. The application of the method is illustrated in the case of a patient with aphasic agraphia, and its general utility is briefly discussed in relation to the nature of various graphic disorders.", "PMID": 973547}
{"id": "pubmed23n0032_9349", "title": "[Cardiomyopathy and rapidly developing proximal myotonic amyotrophy in an athlete (author's transl)].", "content": "The authors report the clinical history of a young athlete who, without any notable family antecedents, developed cardiomyopathy followed by proximal anyotrophy at the age of 24 years. The electromyogram revealed frequent myotonic salves and a typically myogenic tracing. The autopsy showed substantial generalized cardiac enlargement and marked atrophy of the muscle fibres in the girdles, together with grouping of the nuclei into hyperchromatic clusters. The reported case thus shows some characteristics of Steinet's disease and others of non-dystrophic myotonia (Becker, 1964) but cannot be classified as either of these diseases.", "contents": "[Cardiomyopathy and rapidly developing proximal myotonic amyotrophy in an athlete (author's transl)]. The authors report the clinical history of a young athlete who, without any notable family antecedents, developed cardiomyopathy followed by proximal anyotrophy at the age of 24 years. The electromyogram revealed frequent myotonic salves and a typically myogenic tracing. The autopsy showed substantial generalized cardiac enlargement and marked atrophy of the muscle fibres in the girdles, together with grouping of the nuclei into hyperchromatic clusters. The reported case thus shows some characteristics of Steinet's disease and others of non-dystrophic myotonia (Becker, 1964) but cannot be classified as either of these diseases.", "PMID": 973548}
{"id": "pubmed23n0032_9350", "title": "Epileptic seizures in women related to plasma estrogen and progesterone during the menstrual cycle.", "content": "Nine periods in seven women with partial epilepsy have been invetigated with respect to frequency of fits, and estrogen-progesterone levels in blood plasma. Six cycles with ovulation showed a positive correlation between the number of secondary generalized seizures and the mean estrogen/progesterone (E/P) ratios and a negative correlation to plasma progesterone levels. Three periods without ovulation showed an increase in the number of fits during days of high estrogen. The number of fits seemed not to be correlated to changes in body weight.", "contents": "Epileptic seizures in women related to plasma estrogen and progesterone during the menstrual cycle. Nine periods in seven women with partial epilepsy have been invetigated with respect to frequency of fits, and estrogen-progesterone levels in blood plasma. Six cycles with ovulation showed a positive correlation between the number of secondary generalized seizures and the mean estrogen/progesterone (E/P) ratios and a negative correlation to plasma progesterone levels. Three periods without ovulation showed an increase in the number of fits during days of high estrogen. The number of fits seemed not to be correlated to changes in body weight.", "PMID": 973554}
{"id": "pubmed23n0032_9351", "title": "Electroencephalographic examination of 50 women with Turner's syndrome.", "content": "An EEG study has been made of 50 women with Turner's syndrome, 39 found outside institutions and 11 in institutions for the mentally retarded. We found paroxysmal EEG abnormalities in 23 per cent of the former and in 55 per cent of the latter. We found indications of occipital lobe defects in the mentally retarded Turner women.", "contents": "Electroencephalographic examination of 50 women with Turner's syndrome. An EEG study has been made of 50 women with Turner's syndrome, 39 found outside institutions and 11 in institutions for the mentally retarded. We found paroxysmal EEG abnormalities in 23 per cent of the former and in 55 per cent of the latter. We found indications of occipital lobe defects in the mentally retarded Turner women.", "PMID": 973555}
{"id": "pubmed23n0032_9352", "title": "The influence of prostaglandin E2 on the electroencephalogram of normal subjects and epileptic patients.", "content": "In 1973 R.C. Lyneham and others warned against the possible epileptogenic effect of prostaglandins. We studied the influence of this substance on the electroencephalogram of 53 subject (31 non-epileptics and 22 epileptics) before and during the administration of PG E2. Forty-eight tracings remained unchanged, four showed a slight improvement and one showed some worsening during infusion of PG E2. One patient, suffering from daily psychomotor attacks had, accidentally a fit during the trial. It can be concluded that PG E2 does not alter the electroencephalogram in a significant way and that it does not induce epileptic seizures.", "contents": "The influence of prostaglandin E2 on the electroencephalogram of normal subjects and epileptic patients. In 1973 R.C. Lyneham and others warned against the possible epileptogenic effect of prostaglandins. We studied the influence of this substance on the electroencephalogram of 53 subject (31 non-epileptics and 22 epileptics) before and during the administration of PG E2. Forty-eight tracings remained unchanged, four showed a slight improvement and one showed some worsening during infusion of PG E2. One patient, suffering from daily psychomotor attacks had, accidentally a fit during the trial. It can be concluded that PG E2 does not alter the electroencephalogram in a significant way and that it does not induce epileptic seizures.", "PMID": 973549}
{"id": "pubmed23n0032_9353", "title": "[Cerebrospinal fluid shunt in cases of dementia (author's transl)].", "content": "The authors distinguish between two evolutive forms of dementia, one being chracterized by prpgressive deterioration (for instance in the course of arteriopathic, degenerative or infections processes) and the other in which dementia sets in after the clinical picture has improved (for instance after meningeal haemorrhage, craniocerebral injury, endocrinous, metabolic or circulatory disorders, etc.). Ventricular drainage (according to Pudenz-Heyer) was performed in 20 patients presenting dementia: the results were favourable in the seven patients presenting dementia: the results were favourable in the seven patients presenting the first evolutive form and in almost all 13 patients presenting the second form. The pathogenic problems.", "contents": "[Cerebrospinal fluid shunt in cases of dementia (author's transl)]. The authors distinguish between two evolutive forms of dementia, one being chracterized by prpgressive deterioration (for instance in the course of arteriopathic, degenerative or infections processes) and the other in which dementia sets in after the clinical picture has improved (for instance after meningeal haemorrhage, craniocerebral injury, endocrinous, metabolic or circulatory disorders, etc.). Ventricular drainage (according to Pudenz-Heyer) was performed in 20 patients presenting dementia: the results were favourable in the seven patients presenting dementia: the results were favourable in the seven patients presenting the first evolutive form and in almost all 13 patients presenting the second form. The pathogenic problems.", "PMID": 973550}
{"id": "pubmed23n0032_9354", "title": "Cerebral blood flow changes in cluster headache.", "content": "Serial cerebral blood flow studies performed by the intra-carotid 133Xenon method were fortuitously determined during the course of a cluster headache in a 32 year old man. The initial study was performed about 10 min after the headache began and showed values at the upper limit of normal. Twenty min after the headache started a second procedure showed that the autoregulatory response on hyperventilation was normal. Ergotamine tartrate was given intra-muscularly 23 min after the headache began and there was partial relief. A third cerebral blood flow estimation showed abnormally high values. The probable reasons for this are discussed.", "contents": "Cerebral blood flow changes in cluster headache. Serial cerebral blood flow studies performed by the intra-carotid 133Xenon method were fortuitously determined during the course of a cluster headache in a 32 year old man. The initial study was performed about 10 min after the headache began and showed values at the upper limit of normal. Twenty min after the headache started a second procedure showed that the autoregulatory response on hyperventilation was normal. Ergotamine tartrate was given intra-muscularly 23 min after the headache began and there was partial relief. A third cerebral blood flow estimation showed abnormally high values. The probable reasons for this are discussed.", "PMID": 973557}
{"id": "pubmed23n0032_9355", "title": "A prospective study of drugs and pregnancy. 4. Miscellaneous drugs.", "content": "The results are presented of a prospective study on drug use during pregnancy involving antibiotics, analgesic drugs and iron and vitamin preparations. The study was conducted in Malm\u00f6 between 1963 and 1965. No unfavourable effect of the use of antibiotics, mainly penicillin and sulphonamides, could be demonstrated. Among 15 women who had an infant with hypospadias, three had used penicillin during the first trimester, but this may well be coincidental. Analgesic drug use shows a variability which resembles that previously described for psychopharmaca. No effect on the malformation rate or infant survival could be found. A possible lengthening of the mean duration of pregnancy occurred after the use of analgesic drugs during the 2nd or 3rd trimesters. Women who are going to have a dead or malformed infant use iron and/or vitamin preparations less often during late pregnancy than women who prove to have a normal infant. When such drugs were used, the percentage of pregnancies ending in birth before the 38th week is reduced, and the birth weight among term babies is higher. The associations between pregnancy outcome and the use of iron and vitamin preparations is probably indirect, due to social factors associated with drug use.", "contents": "A prospective study of drugs and pregnancy. 4. Miscellaneous drugs. The results are presented of a prospective study on drug use during pregnancy involving antibiotics, analgesic drugs and iron and vitamin preparations. The study was conducted in Malm\u00f6 between 1963 and 1965. No unfavourable effect of the use of antibiotics, mainly penicillin and sulphonamides, could be demonstrated. Among 15 women who had an infant with hypospadias, three had used penicillin during the first trimester, but this may well be coincidental. Analgesic drug use shows a variability which resembles that previously described for psychopharmaca. No effect on the malformation rate or infant survival could be found. A possible lengthening of the mean duration of pregnancy occurred after the use of analgesic drugs during the 2nd or 3rd trimesters. Women who are going to have a dead or malformed infant use iron and/or vitamin preparations less often during late pregnancy than women who prove to have a normal infant. When such drugs were used, the percentage of pregnancies ending in birth before the 38th week is reduced, and the birth weight among term babies is higher. The associations between pregnancy outcome and the use of iron and vitamin preparations is probably indirect, due to social factors associated with drug use.", "PMID": 973559}
{"id": "pubmed23n0032_9356", "title": "Histamine metabolism during the menstrual cycle.", "content": "The urinary excretion of histamine and its metabolites methylhistamine (MeHi) and methylimidazoleacetic acid (MeImAA) was measured during the menstrual cycle in nine healthy women, one allergic woman and three non-pregnant women with anovulatory regular cycles. Simultaneous urinary analyses of luteinizing hormone (LH) and total estrogens were performed. The healthy women showed individual variations in the excretion of histamine, MeHi and MeImAA. This observation has been interpreted as an expression of minor individual differences in the catabolism of histamine. At midcycle an increase in the urinary excretion of histamine metabolites was sometimes evident and a statistically significant correlation could be established between MeHi and estrogen in urine. These results may support previous findings of histamine release by estrogens in uterine tissue but may also reflect an elevated histamine formation. The allergic woman excreted constantly increased amounts of histamine and its metabolites, especially when her allergic symptoms became aggravated pre-menstrually. She did not exhibit any change in MeImAA excretion at midcycle but the MeHi-excretion varied with the excretion of estrogens in the urine. The subjects with anovulatory menstrual cycles had low values of histamine and metabolites although within the normal variations.", "contents": "Histamine metabolism during the menstrual cycle. The urinary excretion of histamine and its metabolites methylhistamine (MeHi) and methylimidazoleacetic acid (MeImAA) was measured during the menstrual cycle in nine healthy women, one allergic woman and three non-pregnant women with anovulatory regular cycles. Simultaneous urinary analyses of luteinizing hormone (LH) and total estrogens were performed. The healthy women showed individual variations in the excretion of histamine, MeHi and MeImAA. This observation has been interpreted as an expression of minor individual differences in the catabolism of histamine. At midcycle an increase in the urinary excretion of histamine metabolites was sometimes evident and a statistically significant correlation could be established between MeHi and estrogen in urine. These results may support previous findings of histamine release by estrogens in uterine tissue but may also reflect an elevated histamine formation. The allergic woman excreted constantly increased amounts of histamine and its metabolites, especially when her allergic symptoms became aggravated pre-menstrually. She did not exhibit any change in MeImAA excretion at midcycle but the MeHi-excretion varied with the excretion of estrogens in the urine. The subjects with anovulatory menstrual cycles had low values of histamine and metabolites although within the normal variations.", "PMID": 973560}
{"id": "pubmed23n0032_9357", "title": "Epidural analgesia in labour. IV. Influence on uterine activity and fetal heart rate.", "content": "The influence of epidural block with bupivacaine (Marcaine-adrenalin) on fetal heart rate, uterine activity, and the frequency and intensity of contractions was studied in twenty-five nulliparae at term. Uterine activity was found to decrease during the first 30 minutes following epidural block. In the time interval 30 to 40 minutes after epidural block uterine activity increased again and attained the same level as during the last 10 minutes before analgesia. The frequency of uterine contractions did not decrease after the block. The lower levels of uterine contractility were due only to a lower amplitude of the contractions. The regularizing effect of epidural analgesia on incoordinate uterine action was noted. The recording of fetal heart rate in the time interval studied revealed no pathological findings. Mode of delivery, mean labour duration and Apgar scores after epidural block were comparable with earlier studies of a larger patient population. This study suggest that epidural analgesia does not induce any important changes in fetal heart rate but temporarily decreases uterine activity.", "contents": "Epidural analgesia in labour. IV. Influence on uterine activity and fetal heart rate. The influence of epidural block with bupivacaine (Marcaine-adrenalin) on fetal heart rate, uterine activity, and the frequency and intensity of contractions was studied in twenty-five nulliparae at term. Uterine activity was found to decrease during the first 30 minutes following epidural block. In the time interval 30 to 40 minutes after epidural block uterine activity increased again and attained the same level as during the last 10 minutes before analgesia. The frequency of uterine contractions did not decrease after the block. The lower levels of uterine contractility were due only to a lower amplitude of the contractions. The regularizing effect of epidural analgesia on incoordinate uterine action was noted. The recording of fetal heart rate in the time interval studied revealed no pathological findings. Mode of delivery, mean labour duration and Apgar scores after epidural block were comparable with earlier studies of a larger patient population. This study suggest that epidural analgesia does not induce any important changes in fetal heart rate but temporarily decreases uterine activity.", "PMID": 973561}
{"id": "pubmed23n0032_9358", "title": "Survey radiography of abdomen following caesarean section, with particular reference to caecal diameter and the presence of free subdiaphragmatic gas.", "content": "The literature concerning perforation and imminent perforation of the caecum is reviewed. The greatest importance is attached to 5 cases of perforation of the caecum associated with paralytic ileus following Caesarean section. The caecal diameter was determined by radiography in 100 patients on the 3rd and 5th day after Caesarean section. At the same time the volume of subdiaphragmatic free gas was assessed. An average caecal diameter of 6.4 cm was found, which is not different from the findings in the normal population. Increasing caecal diameter during the puerperium gives rise to suspicion of the caecum. In 90% of the patients free subdiaphragmatic gas disappeared by the 5th day after Caesarean section.", "contents": "Survey radiography of abdomen following caesarean section, with particular reference to caecal diameter and the presence of free subdiaphragmatic gas. The literature concerning perforation and imminent perforation of the caecum is reviewed. The greatest importance is attached to 5 cases of perforation of the caecum associated with paralytic ileus following Caesarean section. The caecal diameter was determined by radiography in 100 patients on the 3rd and 5th day after Caesarean section. At the same time the volume of subdiaphragmatic free gas was assessed. An average caecal diameter of 6.4 cm was found, which is not different from the findings in the normal population. Increasing caecal diameter during the puerperium gives rise to suspicion of the caecum. In 90% of the patients free subdiaphragmatic gas disappeared by the 5th day after Caesarean section.", "PMID": 973562}
{"id": "pubmed23n0032_9359", "title": "Studies on the mode of action of clomiphene citrate.", "content": "In order to further investigate the mode of action of clomiphene citrate, 5 oophorectomized women in the age range 28 to 41 years were followed with weekly determinations of serum FSH and LH for 20 weeks after the operation. They received no treatment during the first 4 weeks but during the next 16 weeks they were given estradiol 2 mg X 2 daily orally, and from the 12th to the 16th week in addition clomiphene citrate 50 mgX2 daily. The high level of FSH after the oophorectomy decreased gradually during the treatment with estradiol alone, but this effect was partly neutralized by clomiphene citrate, as we observed a significant increase in FSH within 1 week of initiating treatment with clomiphene citrate. On the other hand, we did not find any significant changes in FSH within 4 weeks after treatment with clomiphene citrate was discontinued. The high level of LH after oophorectomy did not show any significant changes during the whole treatment period. It is concluded that clomiphene citrate primarily stimulated FSH secretion, most probably by a competition with estradiol for the receptor sites in the hypothalamus or the pituitary. In addition, it is concluded that clomiphene citrate has a prolonged effect, probably because of a tight binding to the receptor sites.", "contents": "Studies on the mode of action of clomiphene citrate. In order to further investigate the mode of action of clomiphene citrate, 5 oophorectomized women in the age range 28 to 41 years were followed with weekly determinations of serum FSH and LH for 20 weeks after the operation. They received no treatment during the first 4 weeks but during the next 16 weeks they were given estradiol 2 mg X 2 daily orally, and from the 12th to the 16th week in addition clomiphene citrate 50 mgX2 daily. The high level of FSH after the oophorectomy decreased gradually during the treatment with estradiol alone, but this effect was partly neutralized by clomiphene citrate, as we observed a significant increase in FSH within 1 week of initiating treatment with clomiphene citrate. On the other hand, we did not find any significant changes in FSH within 4 weeks after treatment with clomiphene citrate was discontinued. The high level of LH after oophorectomy did not show any significant changes during the whole treatment period. It is concluded that clomiphene citrate primarily stimulated FSH secretion, most probably by a competition with estradiol for the receptor sites in the hypothalamus or the pituitary. In addition, it is concluded that clomiphene citrate has a prolonged effect, probably because of a tight binding to the receptor sites.", "PMID": 973563}
{"id": "pubmed23n0032_9360", "title": "The behaviour of microsomal mono-electron carriers in human placenta during late pregnancy.", "content": "The activities and the content of enzymes involved in mixed function oxidase systems (cytochromes P-450 and b5, NADPH-cytochrome c reductase and NADH cytochrome b5 reductase) in microsomes from 114 human placentae were found to increase from the 34th up to the 38th-39th week of gestation and later to decrease until the 43rd week. Such a process can be related to the following: (i) the phospholipid content of microsomal membranes; (ii) the protein synthesis possibly induced by steroids such as androstenedione and testosterone.", "contents": "The behaviour of microsomal mono-electron carriers in human placenta during late pregnancy. The activities and the content of enzymes involved in mixed function oxidase systems (cytochromes P-450 and b5, NADPH-cytochrome c reductase and NADH cytochrome b5 reductase) in microsomes from 114 human placentae were found to increase from the 34th up to the 38th-39th week of gestation and later to decrease until the 43rd week. Such a process can be related to the following: (i) the phospholipid content of microsomal membranes; (ii) the protein synthesis possibly induced by steroids such as androstenedione and testosterone.", "PMID": 973564}
{"id": "pubmed23n0032_9361", "title": "Amniotic fluid lecithin concentrations in pregnancies complicated by hypertensive disorders and intrauterine growth retardation.", "content": "Amniotic fluid lecithin has been measured in 92 samples from 75 patients with hypertensive disorders and in normotensive pregnancies complicated by intrauterine growth retardation (IUGR). For the group with hypertensive disorders the lecithin concentrations were not significantly different from a reference material at any stage in gestation. Pre-eclampsia associated with moderately elevated blood pressure and complicated by IUGR showed significantly higher lecithin concentrations than any other diagnostic subgroup. The lecithin concentrations showed no relationship to maternal urinary estrogen excretion. The value of serial estrogen measurements in predicting IUGR has been emphasized. An evaluation of the amniotic fluid lecithin concentration in relation to respiratory function has been made. Respiratory distress syndrome may occur even in growth retarded babies.", "contents": "Amniotic fluid lecithin concentrations in pregnancies complicated by hypertensive disorders and intrauterine growth retardation. Amniotic fluid lecithin has been measured in 92 samples from 75 patients with hypertensive disorders and in normotensive pregnancies complicated by intrauterine growth retardation (IUGR). For the group with hypertensive disorders the lecithin concentrations were not significantly different from a reference material at any stage in gestation. Pre-eclampsia associated with moderately elevated blood pressure and complicated by IUGR showed significantly higher lecithin concentrations than any other diagnostic subgroup. The lecithin concentrations showed no relationship to maternal urinary estrogen excretion. The value of serial estrogen measurements in predicting IUGR has been emphasized. An evaluation of the amniotic fluid lecithin concentration in relation to respiratory function has been made. Respiratory distress syndrome may occur even in growth retarded babies.", "PMID": 973565}
{"id": "pubmed23n0032_9362", "title": "Cervical cancer in women belonging to a cytologically screened population.", "content": "Women belonging to the cytologically screened population of Stockholm in 1968 to 1974 and developing cervical cancer of stage I to IV were studied. The purpose was to find out the number of women, in whom the cancer or its preclinical stage was not detected at routine screening, and the reasons for this fact. It was found that 34 out of 177 women had never been cytologically screened. The remaining 143 women had been checked at mass screening and/or at private specialists or hospitals. In 51 screened women the cancer was not detected until the women themselves attended a doctor because of symptoms. Thus in 85 women, or 48% of the series, the cancer escaped detection at an asymptomatic stage. Errors causing a delay or interruption of the follow-up of patients with suspicious smears or colposcopic atypia were observed in 25 cases. Sixty-four patients, or 45% of all screened women, had had at least one negative smear within 4.5 years prior to discovery of the malignancy. Out of these, 53 patients had got a negative smear within 3 years. Whatever might be the true evaluation of these negative smears, their influence on the continued management of the women was important. In view of the results of this study the 4 years interval of rescreening practiced in Stockholm seems to be too long. Moreover the statement is supported, that the value of health screenings is counteracted by the fact that the people most at risk are the least likely to attend.", "contents": "Cervical cancer in women belonging to a cytologically screened population. Women belonging to the cytologically screened population of Stockholm in 1968 to 1974 and developing cervical cancer of stage I to IV were studied. The purpose was to find out the number of women, in whom the cancer or its preclinical stage was not detected at routine screening, and the reasons for this fact. It was found that 34 out of 177 women had never been cytologically screened. The remaining 143 women had been checked at mass screening and/or at private specialists or hospitals. In 51 screened women the cancer was not detected until the women themselves attended a doctor because of symptoms. Thus in 85 women, or 48% of the series, the cancer escaped detection at an asymptomatic stage. Errors causing a delay or interruption of the follow-up of patients with suspicious smears or colposcopic atypia were observed in 25 cases. Sixty-four patients, or 45% of all screened women, had had at least one negative smear within 4.5 years prior to discovery of the malignancy. Out of these, 53 patients had got a negative smear within 3 years. Whatever might be the true evaluation of these negative smears, their influence on the continued management of the women was important. In view of the results of this study the 4 years interval of rescreening practiced in Stockholm seems to be too long. Moreover the statement is supported, that the value of health screenings is counteracted by the fact that the people most at risk are the least likely to attend.", "PMID": 973566}
{"id": "pubmed23n0032_9363", "title": "Outcome of pregnancy after clomiphene therapy.", "content": "Of 159 pregnancies conceived after clomiphene therapy, 141 ended in childbirth, including seven sets of twins. There was a probable increase in the number of infants born with major malformations. These were exclusively to women who had not previously borne a normal infant. The incidence of malformed infants compares well with that published after gonadotropin therapy. The possibly higher incidence of malformations seen after drug-induced ovulation would therefore seem to be due to the underlying subfertility state and thus not a direct drug effect.", "contents": "Outcome of pregnancy after clomiphene therapy. Of 159 pregnancies conceived after clomiphene therapy, 141 ended in childbirth, including seven sets of twins. There was a probable increase in the number of infants born with major malformations. These were exclusively to women who had not previously borne a normal infant. The incidence of malformed infants compares well with that published after gonadotropin therapy. The possibly higher incidence of malformations seen after drug-induced ovulation would therefore seem to be due to the underlying subfertility state and thus not a direct drug effect.", "PMID": 973567}
{"id": "pubmed23n0032_9364", "title": "Dope is to get high: a preliminary analysis of intracultural variation in drug categories among heavy users and dealers.", "content": "Users' definitions and classifications of drugs diverge from officially recognized systems and vary among themselves. They manifest a preoccupation with the experience of being high and reflect the relationship of the drug-using subcultural coalition to the mainstream of American life. Types of categorizations support recent descriptions of types of users reported in the literature as well as indicating areas of diffusion of drug use.", "contents": "Dope is to get high: a preliminary analysis of intracultural variation in drug categories among heavy users and dealers. Users' definitions and classifications of drugs diverge from officially recognized systems and vary among themselves. They manifest a preoccupation with the experience of being high and reflect the relationship of the drug-using subcultural coalition to the mainstream of American life. Types of categorizations support recent descriptions of types of users reported in the literature as well as indicating areas of diffusion of drug use.", "PMID": 973570}
{"id": "pubmed23n0032_9365", "title": "Polyelectrolyte effects in gel chromatography.", "content": "When polyelectrolytes (i.e., lignosulfonates) are eluted from Sepfected by steric exclusion, polyelectrolyte expansion, ion exclusion, and ion inclusion effects. The last-mentioned effect arises from the interaction of charged sites in the high-MW macroions, which are more or less excluded from the gel phase with charged sites in more permeable macroions. The ion inclusion effect depends on the counterion in such a way that the fractionation of polyelectrolytes with a univalent counterion is affected to a greater extent than is the fractionation of polyelectrolytes with a divalent counterion. This is attributable to the existence of a higher density of free charges in the former case, since univalent counterions have a lesser tendency to form ion pairs with the charged sites of the macroion than have divalent counter-ions. The ion inclusion effect may have a favorable influence on the resolution of components of relatively equal molar volume, but of different charge densities. Polyelectrolyte effects are suppressed if the elution is carried oe polyelectrolyte behaves as a nonionic polymer. However, since the separation effected by differences in charge densities is eliminated (swamped out), resolution may deteriorate; nevertheless, the fractionation order is independent of the composition of the sample, the concentration of sample solution, and other fractionation variables. If one wishes to acquire a general picture of the composition of a certain polyelectrolyte, a solution of a simple electrolyte may be the most appropriate eluent. However, if polyelectrolyte components with small differences in hydrodynamic volume are to be separated, an eluent comprising a solution of a high-MW polyelectrolyte, completely excluded by the gel (e.g., dextran sulfate), can be used.", "contents": "Polyelectrolyte effects in gel chromatography. When polyelectrolytes (i.e., lignosulfonates) are eluted from Sepfected by steric exclusion, polyelectrolyte expansion, ion exclusion, and ion inclusion effects. The last-mentioned effect arises from the interaction of charged sites in the high-MW macroions, which are more or less excluded from the gel phase with charged sites in more permeable macroions. The ion inclusion effect depends on the counterion in such a way that the fractionation of polyelectrolytes with a univalent counterion is affected to a greater extent than is the fractionation of polyelectrolytes with a divalent counterion. This is attributable to the existence of a higher density of free charges in the former case, since univalent counterions have a lesser tendency to form ion pairs with the charged sites of the macroion than have divalent counter-ions. The ion inclusion effect may have a favorable influence on the resolution of components of relatively equal molar volume, but of different charge densities. Polyelectrolyte effects are suppressed if the elution is carried oe polyelectrolyte behaves as a nonionic polymer. However, since the separation effected by differences in charge densities is eliminated (swamped out), resolution may deteriorate; nevertheless, the fractionation order is independent of the composition of the sample, the concentration of sample solution, and other fractionation variables. If one wishes to acquire a general picture of the composition of a certain polyelectrolyte, a solution of a simple electrolyte may be the most appropriate eluent. However, if polyelectrolyte components with small differences in hydrodynamic volume are to be separated, an eluent comprising a solution of a high-MW polyelectrolyte, completely excluded by the gel (e.g., dextran sulfate), can be used.", "PMID": 973583}
{"id": "pubmed23n0032_9366", "title": "Vitamin D status of patients with femoral neck fractures.", "content": "Thirty-nine patients admitted to hospital with fractures of the neck of the femur had mean serum 25-hydroxycholecalciferol (25-(OH)D3) levels markedly lower than that of young healthy controls. There was also a significant seasonal variation in these 25-(OH)D3 levels, values in winter being about half those in summer. It is suggested that vitamin D deficiency plays an an important role in the pathology of old age.", "contents": "Vitamin D status of patients with femoral neck fractures. Thirty-nine patients admitted to hospital with fractures of the neck of the femur had mean serum 25-hydroxycholecalciferol (25-(OH)D3) levels markedly lower than that of young healthy controls. There was also a significant seasonal variation in these 25-(OH)D3 levels, values in winter being about half those in summer. It is suggested that vitamin D deficiency plays an an important role in the pathology of old age.", "PMID": 973586}
{"id": "pubmed23n0032_9367", "title": "Ulnar deviation of the fingers as a clinical sign in the elderly.", "content": "A significant percentage of elderly women has been found to have ulnar deviation. As measured at the middle finger, the mean deviation of 73 degrees is small, but nearly 25% of subjects showed a clinically visible deviation of between 11 degrees and 25 degrees. Ulnar deviation has been noted in subjects who are not suffering from the diseases and states commonly regarded as being important to its aetiology. In those it may represent the end-point of the progressive drift considered by some to be a normal ageing process. Whether the aetiology lies here or in one of the other diseases of the locomotor or central nervous systems is uncertain, but caution should be used when interpreting the clinical sign in the elderly.", "contents": "Ulnar deviation of the fingers as a clinical sign in the elderly. A significant percentage of elderly women has been found to have ulnar deviation. As measured at the middle finger, the mean deviation of 73 degrees is small, but nearly 25% of subjects showed a clinically visible deviation of between 11 degrees and 25 degrees. Ulnar deviation has been noted in subjects who are not suffering from the diseases and states commonly regarded as being important to its aetiology. In those it may represent the end-point of the progressive drift considered by some to be a normal ageing process. Whether the aetiology lies here or in one of the other diseases of the locomotor or central nervous systems is uncertain, but caution should be used when interpreting the clinical sign in the elderly.", "PMID": 973587}
{"id": "pubmed23n0032_9368", "title": "Observations on thyroid function tests in the elderly.", "content": "One hundred elderly men and women living in old people's homes and 95 in a long-stay geriatric hospital were investigated for thyroid function by measuring the serum thyroxine, T-3 uptake and calculating the free thyroxine index. The values obtained were found to be within the normal adult range in 97% of the subjects studied. No significant correlation was found between albumin and T-3 uptake, except in men in hospital.", "contents": "Observations on thyroid function tests in the elderly. One hundred elderly men and women living in old people's homes and 95 in a long-stay geriatric hospital were investigated for thyroid function by measuring the serum thyroxine, T-3 uptake and calculating the free thyroxine index. The values obtained were found to be within the normal adult range in 97% of the subjects studied. No significant correlation was found between albumin and T-3 uptake, except in men in hospital.", "PMID": 973588}
{"id": "pubmed23n0032_9369", "title": "Wheat bran in dyschezia in the aged.", "content": "A trial of wheat bran added to the diet of aged dyschezic hospital patients (12 men, 13 women) is reported. This significantly reduced the need for aperients and suppositories but revealed unexpected differences in response by sex. In men, bran produced more bowel actions, fewer constipated days and an increase in stool size. In women there was much less effect upon the number of bowel actions and reduction of constipated days. In men there were fewer constipated days and need for aperients after the bran was withdrawn. Side-effects were only seen in men. Most patients received 15 g/day (range 5-25 g/day). Although cheap and effective in replacing aperients, there were problems in administration and control of incontinence for the nursing staff. The value of added wheat bran in the bowel management of the long-stay hospital patient is discussed and an effective method of combining bran in the diet (e.g. with bread) might be further investigated.", "contents": "Wheat bran in dyschezia in the aged. A trial of wheat bran added to the diet of aged dyschezic hospital patients (12 men, 13 women) is reported. This significantly reduced the need for aperients and suppositories but revealed unexpected differences in response by sex. In men, bran produced more bowel actions, fewer constipated days and an increase in stool size. In women there was much less effect upon the number of bowel actions and reduction of constipated days. In men there were fewer constipated days and need for aperients after the bran was withdrawn. Side-effects were only seen in men. Most patients received 15 g/day (range 5-25 g/day). Although cheap and effective in replacing aperients, there were problems in administration and control of incontinence for the nursing staff. The value of added wheat bran in the bowel management of the long-stay hospital patient is discussed and an effective method of combining bran in the diet (e.g. with bread) might be further investigated.", "PMID": 973589}
{"id": "pubmed23n0032_9370", "title": "Effect of a lathyrogen (aminoacetonitrile hydrochloride) on aortic physiosclerosis and skeletal mineralization in growing rats of different age.", "content": "In one experiment, four groups of six-week-old-male PVG rats were, respectively, given tap water ('control group'), 0.00125M, 0.0025M, and 0.005M lathyrogen (aminoacetonitrile hydrochloride: AAN) solutions ad libitum for a period of 20 weeks. In a second experiment, 12-week-old male rats were similarly treated. It was found that the younger rats were far more susceptible to lathyrogenic influences than the older rats. For example, the rate of aortic physiosclerosis was significantly retarded in the six-week-old rats at dosages of 0.00125M and 0.0025M (the six-week-old rats exposed to 0.005M AAN were eliminated from the experiment before its completion), whilst physiosclerosis was unaffected even in those 12-week-old rats exposed to 0.005M AAN. The degree of mineralization of the femur (as reflected by its ash, Ca, Mg, Na, and K content) was retarded in approximately dose-dependent fashion in the rats of both ages, the effect being somewhat more pronounced in the younger animals. These results were discussed in the context of the known effects of lathyrogenic compounds on the biochemical maturation of connective tissue components, and also in the context of previous attempts to modify the lifespan of laboratory animals by lathyrogen administration.", "contents": "Effect of a lathyrogen (aminoacetonitrile hydrochloride) on aortic physiosclerosis and skeletal mineralization in growing rats of different age. In one experiment, four groups of six-week-old-male PVG rats were, respectively, given tap water ('control group'), 0.00125M, 0.0025M, and 0.005M lathyrogen (aminoacetonitrile hydrochloride: AAN) solutions ad libitum for a period of 20 weeks. In a second experiment, 12-week-old male rats were similarly treated. It was found that the younger rats were far more susceptible to lathyrogenic influences than the older rats. For example, the rate of aortic physiosclerosis was significantly retarded in the six-week-old rats at dosages of 0.00125M and 0.0025M (the six-week-old rats exposed to 0.005M AAN were eliminated from the experiment before its completion), whilst physiosclerosis was unaffected even in those 12-week-old rats exposed to 0.005M AAN. The degree of mineralization of the femur (as reflected by its ash, Ca, Mg, Na, and K content) was retarded in approximately dose-dependent fashion in the rats of both ages, the effect being somewhat more pronounced in the younger animals. These results were discussed in the context of the known effects of lathyrogenic compounds on the biochemical maturation of connective tissue components, and also in the context of previous attempts to modify the lifespan of laboratory animals by lathyrogen administration.", "PMID": 973590}
{"id": "pubmed23n0032_9371", "title": "Geriatric day hospitals: counting the cost compared with other methods of support.", "content": "The past decade has seen a great expansion of the day-hospital element in geriatric and psychiatric care, and the merits of such therapeutic agencies have been described quite often--but with little reference to the economic benefit to the community. We have described an enquiry into the true cost of a typical 'intermediate' day hospital and introduced valid comparisons with other methods of caring for the elderly sick or frail: these comparisons inevitably led to the inclusion of the acute general hospital when considering the integrated health and welfare service which we require. The surprising financial implications should be recognized as we enter another era of stringency which must restrict development of the National Health Service.", "contents": "Geriatric day hospitals: counting the cost compared with other methods of support. The past decade has seen a great expansion of the day-hospital element in geriatric and psychiatric care, and the merits of such therapeutic agencies have been described quite often--but with little reference to the economic benefit to the community. We have described an enquiry into the true cost of a typical 'intermediate' day hospital and introduced valid comparisons with other methods of caring for the elderly sick or frail: these comparisons inevitably led to the inclusion of the acute general hospital when considering the integrated health and welfare service which we require. The surprising financial implications should be recognized as we enter another era of stringency which must restrict development of the National Health Service.", "PMID": 973591}
{"id": "pubmed23n0032_9372", "title": "Follow-up study of two hundred admissions to a residential home.", "content": "Two hundred admissions to a local-authority Residential Home in Edinburgh were followed up for four years to study subsequent morbidity and mortality in the Home or after transfer. Their survival was compared with that of patients transferred from a Geriatric Assessment Unit to Long-stay Units. The mortality of both groups was greater than expected, particularly in the early weeks and in those suffering from cerebrovascular disease.", "contents": "Follow-up study of two hundred admissions to a residential home. Two hundred admissions to a local-authority Residential Home in Edinburgh were followed up for four years to study subsequent morbidity and mortality in the Home or after transfer. Their survival was compared with that of patients transferred from a Geriatric Assessment Unit to Long-stay Units. The mortality of both groups was greater than expected, particularly in the early weeks and in those suffering from cerebrovascular disease.", "PMID": 973592}
{"id": "pubmed23n0032_9373", "title": "Cephazolin treatment of pneumonia in the elderly.", "content": "Cephazolin, a semi-synthetic cephalosporin for parenteral use, was evaluated in 39 elderly hospital patients. Thirty-three of these patients were seriously ill at the start of treatment, suffering from pulmonary infections. In the other six patients, the drug was used post-operatively as a prophylactic, and it was effective in all cases in preventing any subsequent infection. Cephazolin was very effective in the treatment of 27 of the severely ill patients; the primary pathogen was eradicated and there was good clinical improvement. In two patients the primary pathogens were resistant to cephazolin, and the antibiotic therapy was changed after sensitivities were known. Of four patients with Haemophilus influenzae infection, clinical cure was obtained in two. Cephazolin therapy was discontinued in one woman because she developed a rash. However, there were no major toxic effects of therapy in terms of hepatic, renal or haematological function. No patient complained of pain when the intramuscular therapy was given. A dose of 1 g IM twice daily was shown to produce consistently high serum concentrations. Thus, in the elderly, the advantages of cephazolin are its lack of nephrotoxicity even when diuretic therapy is being administered concurrently, its lack of pain on intramuscular injection, and its sustained concentrations in the blood and urine so that it only requires to be given twice daily. In vitro studies showed that cephazolin is more active than cephaloridine against hospital pathogens.", "contents": "Cephazolin treatment of pneumonia in the elderly. Cephazolin, a semi-synthetic cephalosporin for parenteral use, was evaluated in 39 elderly hospital patients. Thirty-three of these patients were seriously ill at the start of treatment, suffering from pulmonary infections. In the other six patients, the drug was used post-operatively as a prophylactic, and it was effective in all cases in preventing any subsequent infection. Cephazolin was very effective in the treatment of 27 of the severely ill patients; the primary pathogen was eradicated and there was good clinical improvement. In two patients the primary pathogens were resistant to cephazolin, and the antibiotic therapy was changed after sensitivities were known. Of four patients with Haemophilus influenzae infection, clinical cure was obtained in two. Cephazolin therapy was discontinued in one woman because she developed a rash. However, there were no major toxic effects of therapy in terms of hepatic, renal or haematological function. No patient complained of pain when the intramuscular therapy was given. A dose of 1 g IM twice daily was shown to produce consistently high serum concentrations. Thus, in the elderly, the advantages of cephazolin are its lack of nephrotoxicity even when diuretic therapy is being administered concurrently, its lack of pain on intramuscular injection, and its sustained concentrations in the blood and urine so that it only requires to be given twice daily. In vitro studies showed that cephazolin is more active than cephaloridine against hospital pathogens.", "PMID": 973593}
{"id": "pubmed23n0032_9374", "title": "The stricken: the social consequences of stroke.", "content": "Of 76 patients admitted consecutively to a stroke rehabilitation ward at Lightburn Hospital Glasgow, 35 were discharged home, and 29 of these were followed at home for three years or until they died. Eighteen of the patients survived for three years, the survivors being mainly the younger patients. Most remained reasonably fit physically, but none returned to employment and few undertook any activities outside the house. Many became depressed and frustrated. This group received little in the way of domiciliary health and social services, rehabilitation services and financial help. There is a need to improve the services for survivors of strokes after their discharge from hospital.", "contents": "The stricken: the social consequences of stroke. Of 76 patients admitted consecutively to a stroke rehabilitation ward at Lightburn Hospital Glasgow, 35 were discharged home, and 29 of these were followed at home for three years or until they died. Eighteen of the patients survived for three years, the survivors being mainly the younger patients. Most remained reasonably fit physically, but none returned to employment and few undertook any activities outside the house. Many became depressed and frustrated. This group received little in the way of domiciliary health and social services, rehabilitation services and financial help. There is a need to improve the services for survivors of strokes after their discharge from hospital.", "PMID": 973594}
{"id": "pubmed23n0032_9375", "title": "The effect of parity and time between pregnancies on maternal hair chromium concentration.", "content": "Values for hair chromium concentration of nulliparous women and parous women who had just given birth to a child were determined by atomic absorption spectrophotometry. Mean values for hair chromium concentration of nulliparous and parous subjects were 309 +/- 23 and 117 +/- 10 ppb, respectively. The difference was highly significant (P less than 0.001). No further significant decrease in hair chromium was observed in women who had borne more than one child. Hair chromium concentration increased significantly with the amount of time between pregnancies, especially when at least 4 years had passed since the end of the last pregnancy (P less than 0.001). These data suggest a state of suboptimal chromium nutrition during pregnancy.", "contents": "The effect of parity and time between pregnancies on maternal hair chromium concentration. Values for hair chromium concentration of nulliparous women and parous women who had just given birth to a child were determined by atomic absorption spectrophotometry. Mean values for hair chromium concentration of nulliparous and parous subjects were 309 +/- 23 and 117 +/- 10 ppb, respectively. The difference was highly significant (P less than 0.001). No further significant decrease in hair chromium was observed in women who had borne more than one child. Hair chromium concentration increased significantly with the amount of time between pregnancies, especially when at least 4 years had passed since the end of the last pregnancy (P less than 0.001). These data suggest a state of suboptimal chromium nutrition during pregnancy.", "PMID": 973597}
{"id": "pubmed23n0032_9376", "title": "Wine versus ethanol in human nutrition. I. Nitrogen and calorie balance.", "content": "There are implications in the literature that wine is different from other alcoholic beverages and that it may even have a beneficial effect on the nutritional process. A metabolic study was undertaken in an attempt to document the effects of wine versus ethanol on absorption of various nutrients. Nitrogen and caloric data are presented here. During each of four 18-day experimental periods, six healthy, young men were given, in random order, a liter per day of the following test beverages: Zinfandel wine (9.3% w/v alcohol); dealcoholized Zinfandel wine; pure ethanol (9.3% w/v aqueous alcohol solution); and deionized water. These beverages were divided into four equal feedings and administered with a carefully controlled isocaloric diet over a 12-hr period. The subjects tended to lose weight on alcohol-containing regimens, suggesting that calories from alcohol may not be as efficient as those from fat and carbohydrate. Urinary excretion of nitrogen was significantly greater during wine and ethanol administration than during feeding of the other test beverages. This was reflected in an increase in uric acid and urea nitrogen output, primarily, the latter, suggesting that alcohol may directly affect protein catabolism. There was no significant difference in fecal nitrogen excretion between experimental periods.", "contents": "Wine versus ethanol in human nutrition. I. Nitrogen and calorie balance. There are implications in the literature that wine is different from other alcoholic beverages and that it may even have a beneficial effect on the nutritional process. A metabolic study was undertaken in an attempt to document the effects of wine versus ethanol on absorption of various nutrients. Nitrogen and caloric data are presented here. During each of four 18-day experimental periods, six healthy, young men were given, in random order, a liter per day of the following test beverages: Zinfandel wine (9.3% w/v alcohol); dealcoholized Zinfandel wine; pure ethanol (9.3% w/v aqueous alcohol solution); and deionized water. These beverages were divided into four equal feedings and administered with a carefully controlled isocaloric diet over a 12-hr period. The subjects tended to lose weight on alcohol-containing regimens, suggesting that calories from alcohol may not be as efficient as those from fat and carbohydrate. Urinary excretion of nitrogen was significantly greater during wine and ethanol administration than during feeding of the other test beverages. This was reflected in an increase in uric acid and urea nitrogen output, primarily, the latter, suggesting that alcohol may directly affect protein catabolism. There was no significant difference in fecal nitrogen excretion between experimental periods.", "PMID": 973598}
{"id": "pubmed23n0032_9377", "title": "25-Hydroxycholecalciferol levels in bedouins in the Negev.", "content": "25-Hydroxycholecalciferol (25-HCC) levels were measured in 31 bedouin females and eight bedouin male tribesmen and compared with the levels in Jewish males and females in Beersheba. In nonpregnant bedouin women the mean 25-HCC level was 25.4 ng/ml +/- 9.78. In pregnant bedouin women the mean was 23.4 ng/ml +/- 8.52. In bedouin males the mean level was 25.7 ng/ml +/- 3.03. In Jewish females, both pregnant and nonpregnant, the levels were higher (32.7 ng/ml +/- 6.02 and 44.3 ng/ml +/- 9.24). Jewish males had levels of 32.8 +/- 6.29 ng/ml. No bedouin had plasma levels below 10 ng/ml, and there was no evidence to suggest deficiency of vitamin D in bedouin males or females.", "contents": "25-Hydroxycholecalciferol levels in bedouins in the Negev. 25-Hydroxycholecalciferol (25-HCC) levels were measured in 31 bedouin females and eight bedouin male tribesmen and compared with the levels in Jewish males and females in Beersheba. In nonpregnant bedouin women the mean 25-HCC level was 25.4 ng/ml +/- 9.78. In pregnant bedouin women the mean was 23.4 ng/ml +/- 8.52. In bedouin males the mean level was 25.7 ng/ml +/- 3.03. In Jewish females, both pregnant and nonpregnant, the levels were higher (32.7 ng/ml +/- 6.02 and 44.3 ng/ml +/- 9.24). Jewish males had levels of 32.8 +/- 6.29 ng/ml. No bedouin had plasma levels below 10 ng/ml, and there was no evidence to suggest deficiency of vitamin D in bedouin males or females.", "PMID": 973599}
{"id": "pubmed23n0032_9378", "title": "Calorigenic response in obese and nonobese women.", "content": "The calorigenic response to a high protein test meal was studied in women with a history of childhood onset obesity. Obese and nonobese individuals were fasted overnight and basal oxygen consumptions determined the following morning. A semisynthetic 823 kcal high protein test meal was ingested within a 1-hr period followed by hourly determinations of oxygen consumption and plasma levels of insulin, free fatty acids, triglycerides, glucose, amino acids, and urea nitrogen. Amino acid levels increased faster and to a higher plateau in the nonobese group, while insulin levels increased and eventually decreased in a similar fashion in both groups. No differences in basal metabolic rate were detected. The postprandial increment in oxygen consumption was significantly less among the obese subjects. Diminished calorigenesis after each meal would result in accumulation of extra calories provided that caloric consumption is not appropriately decreased. These data suggest that a physiological aberration of energy metabolism may contribute to the development of childhood onset obesity.", "contents": "Calorigenic response in obese and nonobese women. The calorigenic response to a high protein test meal was studied in women with a history of childhood onset obesity. Obese and nonobese individuals were fasted overnight and basal oxygen consumptions determined the following morning. A semisynthetic 823 kcal high protein test meal was ingested within a 1-hr period followed by hourly determinations of oxygen consumption and plasma levels of insulin, free fatty acids, triglycerides, glucose, amino acids, and urea nitrogen. Amino acid levels increased faster and to a higher plateau in the nonobese group, while insulin levels increased and eventually decreased in a similar fashion in both groups. No differences in basal metabolic rate were detected. The postprandial increment in oxygen consumption was significantly less among the obese subjects. Diminished calorigenesis after each meal would result in accumulation of extra calories provided that caloric consumption is not appropriately decreased. These data suggest that a physiological aberration of energy metabolism may contribute to the development of childhood onset obesity.", "PMID": 973600}
{"id": "pubmed23n0032_9379", "title": "A longitudinal study of the protein, nitrogen, and lactose contents of human milk from Swedish well-nourished mothers.", "content": "The contents of total nitrogen, nonprotein nitrogen, lactose, and individual milk proteins have been determined in human milk from well-nourished Swedish mothers. Breast milk samples from 50 mothers at different stages of lactation (up to 170 days) were collected. Furthermore, three mothers gave samples repeatedly throughout the whole lactation period. The protein content in mature milk was found to be 0.8 to 0.9% by amino acid analysis. The nitrogen content and the contents of the major human milk whey proteins, alpha-lactalbumin and lactoferrin, are very high for the first few days, then decrease rapidly and reach, thereafter, the more slowly declining level of mature milk. Nonprotein nitrogen and the nonspecific milk protein serum albumin are present in constant concentrations throughout lactation. The daily milk volumes were determined and found to be 500 to 600 ml in the very early part and 700 to 800 ml in the later part of the lactation period.", "contents": "A longitudinal study of the protein, nitrogen, and lactose contents of human milk from Swedish well-nourished mothers. The contents of total nitrogen, nonprotein nitrogen, lactose, and individual milk proteins have been determined in human milk from well-nourished Swedish mothers. Breast milk samples from 50 mothers at different stages of lactation (up to 170 days) were collected. Furthermore, three mothers gave samples repeatedly throughout the whole lactation period. The protein content in mature milk was found to be 0.8 to 0.9% by amino acid analysis. The nitrogen content and the contents of the major human milk whey proteins, alpha-lactalbumin and lactoferrin, are very high for the first few days, then decrease rapidly and reach, thereafter, the more slowly declining level of mature milk. Nonprotein nitrogen and the nonspecific milk protein serum albumin are present in constant concentrations throughout lactation. The daily milk volumes were determined and found to be 500 to 600 ml in the very early part and 700 to 800 ml in the later part of the lactation period.", "PMID": 973601}
{"id": "pubmed23n0032_9380", "title": "Breast milk composition in Ethiopian and Swedish mothers. II. Lactose, nitrogen, and protein contents.", "content": "Breast milk from underprivileged and privileged Ethiopian mothers was collected at different stages of lactation and analyzed for total nitrogen, nonprotein nitrogen, lactose, and individual milk proteins (lactoferrin, alpha-lactalbumin, serum albumin, IgG and IgM). These values and the milk volume of one meal were compared to corresponding results from well-nourished Swedish mothers. No significant differences between the levels of these constituents or the milk volumes were found between the two groups of Ethiopian mothers. When comparison was made between breast milk from these two Ethiopian groups and the Swedish group, the former two showed significantly higher values for the iron-binding protein lactoferrin.", "contents": "Breast milk composition in Ethiopian and Swedish mothers. II. Lactose, nitrogen, and protein contents. Breast milk from underprivileged and privileged Ethiopian mothers was collected at different stages of lactation and analyzed for total nitrogen, nonprotein nitrogen, lactose, and individual milk proteins (lactoferrin, alpha-lactalbumin, serum albumin, IgG and IgM). These values and the milk volume of one meal were compared to corresponding results from well-nourished Swedish mothers. No significant differences between the levels of these constituents or the milk volumes were found between the two groups of Ethiopian mothers. When comparison was made between breast milk from these two Ethiopian groups and the Swedish group, the former two showed significantly higher values for the iron-binding protein lactoferrin.", "PMID": 973602}
{"id": "pubmed23n0032_9381", "title": "Food iron absorption in human subjects. IV. The effects of calcium and phosphate salts on the absorption of nonheme iron.", "content": "The influence of physiological levels of calcium and phosphorus on the absorption of nonheme iron from a semisynthetic meal was evaluated. Each of the 34 participating subjects received two to four test meals. In three studies where both calcium and phosphate were added, absorption of nonheme iron was reduced to 27 to 47% of that absorbed when no salts were added. However, with the single addition of either calcium or phosphate to the test meals no significantly inhibiting effect was observed. As the absorption of nonheme iron was significantly reduced only with the combined addition of calcium and phosphate, it is suggested that a calcium-phosphate-iron complex forms which inhibits iron absorption.", "contents": "Food iron absorption in human subjects. IV. The effects of calcium and phosphate salts on the absorption of nonheme iron. The influence of physiological levels of calcium and phosphorus on the absorption of nonheme iron from a semisynthetic meal was evaluated. Each of the 34 participating subjects received two to four test meals. In three studies where both calcium and phosphate were added, absorption of nonheme iron was reduced to 27 to 47% of that absorbed when no salts were added. However, with the single addition of either calcium or phosphate to the test meals no significantly inhibiting effect was observed. As the absorption of nonheme iron was significantly reduced only with the combined addition of calcium and phosphate, it is suggested that a calcium-phosphate-iron complex forms which inhibits iron absorption.", "PMID": 973603}
{"id": "pubmed23n0032_9382", "title": "The incidence of anemia in residents of Missouri.", "content": "A state-wide nutritional health survey of Missouri residents conducted in 1973 included hemoglobin, hematocrit, and serum iron determinations on approximately 1,164 persons and dietary iron intake estimates, based on a diet history, for 530 persons. Based on the criteria used for interpretation of the Ten-State Nutrition Survey data, over 19% of all preschool age white children and over 10% of the white children six to 10 years old had low or deficient hemoglobin levels. Between 8 and 17% of the white males between 10 and 60 years old and over 30% of the white males over 59 years old were anemic. Between 3 and 9% of all white females over 9 years of age had low or deficient hemoglobin levels. The levels of anemia for most age and sex groups of Negroes were at least twice as great as for corresponding groups of white persons. Low or deficient hemoglobin levels within the age and sex groups were associated with low hematocrit and serum iron levels; suggesting that some of the anemia was a reflection of iron deficiency. Estimates of the dietary intake of iron indicated that less that 20% of the females 10 to 35 years old and less than 60% of older females and boys under 17 years of age were consuming the Recommended Dietary Allowances. Over 35% of the preschool children and females between 10 and 60 years old were consuming less than two-thirds of their Recommended Dietary Allowances for iron. At least part of the iron deficiency was, therefore, indicated to be related to insufficient dietary intake.", "contents": "The incidence of anemia in residents of Missouri. A state-wide nutritional health survey of Missouri residents conducted in 1973 included hemoglobin, hematocrit, and serum iron determinations on approximately 1,164 persons and dietary iron intake estimates, based on a diet history, for 530 persons. Based on the criteria used for interpretation of the Ten-State Nutrition Survey data, over 19% of all preschool age white children and over 10% of the white children six to 10 years old had low or deficient hemoglobin levels. Between 8 and 17% of the white males between 10 and 60 years old and over 30% of the white males over 59 years old were anemic. Between 3 and 9% of all white females over 9 years of age had low or deficient hemoglobin levels. The levels of anemia for most age and sex groups of Negroes were at least twice as great as for corresponding groups of white persons. Low or deficient hemoglobin levels within the age and sex groups were associated with low hematocrit and serum iron levels; suggesting that some of the anemia was a reflection of iron deficiency. Estimates of the dietary intake of iron indicated that less that 20% of the females 10 to 35 years old and less than 60% of older females and boys under 17 years of age were consuming the Recommended Dietary Allowances. Over 35% of the preschool children and females between 10 and 60 years old were consuming less than two-thirds of their Recommended Dietary Allowances for iron. At least part of the iron deficiency was, therefore, indicated to be related to insufficient dietary intake.", "PMID": 973604}
{"id": "pubmed23n0032_9383", "title": "Some factors related to obesity in the Japanese sumo wrestler.", "content": "Sumo is an ancient sport in Japan and there are at present over 800 professional sumo wrestlers (rikishis). After entrance into the wrestler society a wrestler takes strenuous daily training together with a very high calorie diet (more than 5,000 cal). Frequency of food intake is twice a day. The average diet of Japanese people contains of 2,279 calories and the meal frequency is generally three times a day. In 96 wrestlers average actual body weight and modified Broca index was 100.4 kg and 143.5%, respectively. In this group the prevalence of overweight with obesity, overweight without obesity, nonoverweight with obesity, and nonoverweight without obesity was 53.4, 39.1, 1.0, and 6.5%, respectively. Also mean serum levels of triglyceride, phospholipid, uric acid, and total protein were significantly higher than those obtained in 89 age-matched healthy males. The incidence of diabetes mellitus, gout, and hypertension in wrestlers was 5.2, 6.3, and 8.3%, respectively, all values being considerably higher than in controls. Weight correlated significantly with skinfold thickness, diastolic blood pressure, total cholesterol, and uric acid in each group. Multiple regression analyses were made treating weight or uric acid as dependent variables in both groups. Obesity, hyperlipidemia, and hyperuricemia in wrestlers were presumed to be caused chiefly by the high calorie diet and partially by the infrequent meal intake.", "contents": "Some factors related to obesity in the Japanese sumo wrestler. Sumo is an ancient sport in Japan and there are at present over 800 professional sumo wrestlers (rikishis). After entrance into the wrestler society a wrestler takes strenuous daily training together with a very high calorie diet (more than 5,000 cal). Frequency of food intake is twice a day. The average diet of Japanese people contains of 2,279 calories and the meal frequency is generally three times a day. In 96 wrestlers average actual body weight and modified Broca index was 100.4 kg and 143.5%, respectively. In this group the prevalence of overweight with obesity, overweight without obesity, nonoverweight with obesity, and nonoverweight without obesity was 53.4, 39.1, 1.0, and 6.5%, respectively. Also mean serum levels of triglyceride, phospholipid, uric acid, and total protein were significantly higher than those obtained in 89 age-matched healthy males. The incidence of diabetes mellitus, gout, and hypertension in wrestlers was 5.2, 6.3, and 8.3%, respectively, all values being considerably higher than in controls. Weight correlated significantly with skinfold thickness, diastolic blood pressure, total cholesterol, and uric acid in each group. Multiple regression analyses were made treating weight or uric acid as dependent variables in both groups. Obesity, hyperlipidemia, and hyperuricemia in wrestlers were presumed to be caused chiefly by the high calorie diet and partially by the infrequent meal intake.", "PMID": 973605}
{"id": "pubmed23n0032_9384", "title": "Food and children with Prader-Willi syndrome.", "content": "Observations regarding caloric intake, growth, and food-related behavior of 14 children with Prader-Willi syndrome have shown that they need fewer calories than normal children to maintain their weight in an appropriate weight channel, and that they exhibit peculiar food-related behavior that in some instances is present before the onset of obesity. Most of them can be prevented from gaining excessive weight or can lose weight if they and their caretakers receive appropriate counseling on how to provide low-calorie food and how to make other food inaccessible. In 12 of the 14 children, a reasonably successful weight-control program was achieved for up to five years. A biological basis for many of the noted observations is assumed, but the exact nature of the defect remains elusive.", "contents": "Food and children with Prader-Willi syndrome. Observations regarding caloric intake, growth, and food-related behavior of 14 children with Prader-Willi syndrome have shown that they need fewer calories than normal children to maintain their weight in an appropriate weight channel, and that they exhibit peculiar food-related behavior that in some instances is present before the onset of obesity. Most of them can be prevented from gaining excessive weight or can lose weight if they and their caretakers receive appropriate counseling on how to provide low-calorie food and how to make other food inaccessible. In 12 of the 14 children, a reasonably successful weight-control program was achieved for up to five years. A biological basis for many of the noted observations is assumed, but the exact nature of the defect remains elusive.", "PMID": 973608}
{"id": "pubmed23n0032_9385", "title": "Perinatal methylmercury poisoning in Iraq.", "content": "The signs and symptoms of methylmercury poisoning and the concentrations of mercury in samples of blood, hair, and milk are reported and compared in two infant-mother pairs exposed in the recent Iraq outbreak. In one pair, the infant was born prior to the exposure of the mother, and was exposed only from ingestion of methylmercury in mother's milk. In the other pair, the mother was exposed during pregnancy and did not breast feed the infant, who died 30 days after birth. Both mothers had some signs and symptoms of poisoning, but the infants did not. The infants had maximum estimated blood mercury levels between 500 and 1,000 ng/ml, well above the minimum toxic levels for adults. Hair and blood concentrations of mercury correlated closely, the average hair-blood ratio being about 250. Maternal milk averaged 8.6% of the simultaneous blood level, but the relationship was nonlinear at blood mercury levels below 50 ng/ml. Postnatal exposure by suckling led to substantial infant blood mercury concentration (over 600 ng/ml), and prenatal exposure resulted in blood mercury levels in the infant at birth higher than those in the mother, even after corrections for differences in hematocrit level.", "contents": "Perinatal methylmercury poisoning in Iraq. The signs and symptoms of methylmercury poisoning and the concentrations of mercury in samples of blood, hair, and milk are reported and compared in two infant-mother pairs exposed in the recent Iraq outbreak. In one pair, the infant was born prior to the exposure of the mother, and was exposed only from ingestion of methylmercury in mother's milk. In the other pair, the mother was exposed during pregnancy and did not breast feed the infant, who died 30 days after birth. Both mothers had some signs and symptoms of poisoning, but the infants did not. The infants had maximum estimated blood mercury levels between 500 and 1,000 ng/ml, well above the minimum toxic levels for adults. Hair and blood concentrations of mercury correlated closely, the average hair-blood ratio being about 250. Maternal milk averaged 8.6% of the simultaneous blood level, but the relationship was nonlinear at blood mercury levels below 50 ng/ml. Postnatal exposure by suckling led to substantial infant blood mercury concentration (over 600 ng/ml), and prenatal exposure resulted in blood mercury levels in the infant at birth higher than those in the mother, even after corrections for differences in hematocrit level.", "PMID": 973609}
{"id": "pubmed23n0032_9386", "title": "Serologic response to revaccination with two rubella vaccines.", "content": "Three years after receiving rubella vaccine, 1,060 elementary school children living on the island of Maui, Hawaii, were revaccinated with either HPV-77 DE-5 or RA 27/3 rubella vaccine given subcutaneously or intranasally in order to compare the effectiveness of these two vaccines in raising antibody titers. RA 27/3 was the more effective booster vaccine, producing fourfold or greater titer rises in 20.1% of recipients, including 80% of children with hemagglutination-inhibiting antibody titers less than or equal to 1:40 at the time of revaccination, intranasal revaccination was not significantly more effective than subcutaneous revaccination, although it did elicit higher titers in children who responded. Responses differed according to the vaccine that children had received three years earlier. Because antibody titers have persisted in vaccinated children, routine administration of a second dose of rubella vaccine is not currently recommended.", "contents": "Serologic response to revaccination with two rubella vaccines. Three years after receiving rubella vaccine, 1,060 elementary school children living on the island of Maui, Hawaii, were revaccinated with either HPV-77 DE-5 or RA 27/3 rubella vaccine given subcutaneously or intranasally in order to compare the effectiveness of these two vaccines in raising antibody titers. RA 27/3 was the more effective booster vaccine, producing fourfold or greater titer rises in 20.1% of recipients, including 80% of children with hemagglutination-inhibiting antibody titers less than or equal to 1:40 at the time of revaccination, intranasal revaccination was not significantly more effective than subcutaneous revaccination, although it did elicit higher titers in children who responded. Responses differed according to the vaccine that children had received three years earlier. Because antibody titers have persisted in vaccinated children, routine administration of a second dose of rubella vaccine is not currently recommended.", "PMID": 973610}
{"id": "pubmed23n0032_9387", "title": "Evaluation of Wistar RA27/3 rubella virus vaccine in children.", "content": "Because the Wistar RA27/3 strain rubella virus vaccine has potentially important advantages over rubella vaccines currently licensed in the United States, a field trial was conducted in Minnesota and Wisconsin in 1974 to evaluate RA27/3 in this country. Two hundred eighty-five (99.7%) of 286 susceptible children given RA27/3 subcutaneously seroconverted, with a geometric mean hemagglutination inhibition (HI) titer of 81.2. Twenty-eight (23%) of 122 children with rubella antibodies before immunization had fourfold or greater rises in rubella HI titers. The highest percentage of booster responses occurred in children with low preimmunization titers. Side effects were reported in 34% of subjects, but only one reaction associated with RA27/3 was serious: in a 5-year-old boy, arthritis of the left hip developed 31 days after immunization. This study indicates that RA27/3 vaccine produced a very high rate of seroconversion with high postimmunization HI titers. The ability to elicit significant booster responses in children with low levels of HI antibodies suggests that RA27/3 could be used to boost immunity in women of childbearing age whose rubella titers have declined to undetectable levels.", "contents": "Evaluation of Wistar RA27/3 rubella virus vaccine in children. Because the Wistar RA27/3 strain rubella virus vaccine has potentially important advantages over rubella vaccines currently licensed in the United States, a field trial was conducted in Minnesota and Wisconsin in 1974 to evaluate RA27/3 in this country. Two hundred eighty-five (99.7%) of 286 susceptible children given RA27/3 subcutaneously seroconverted, with a geometric mean hemagglutination inhibition (HI) titer of 81.2. Twenty-eight (23%) of 122 children with rubella antibodies before immunization had fourfold or greater rises in rubella HI titers. The highest percentage of booster responses occurred in children with low preimmunization titers. Side effects were reported in 34% of subjects, but only one reaction associated with RA27/3 was serious: in a 5-year-old boy, arthritis of the left hip developed 31 days after immunization. This study indicates that RA27/3 vaccine produced a very high rate of seroconversion with high postimmunization HI titers. The ability to elicit significant booster responses in children with low levels of HI antibodies suggests that RA27/3 could be used to boost immunity in women of childbearing age whose rubella titers have declined to undetectable levels.", "PMID": 973611}
{"id": "pubmed23n0032_9388", "title": "Vaccination caveat. The off-the-shoulder look.", "content": "To avoid the difficult problem of upper shoulder scarring, a plea is made for vaccination in the lower deltoid region.", "contents": "Vaccination caveat. The off-the-shoulder look. To avoid the difficult problem of upper shoulder scarring, a plea is made for vaccination in the lower deltoid region.", "PMID": 973612}
{"id": "pubmed23n0032_9389", "title": "Benign paroxysmal vertigo of childhood.", "content": "Thirty-three cases of benign paroxysmal vertigo in childhood have been seen at our institution since the disorder was recognized ten years ago. Progression from paroxysmal torticollis of infancy to paroxysmal vertigo of childhood is documented. Ear infections and allergy appeared causative in a few, but not most, of the cases. The most important consideration for the pediatrician is to rule out epilepsy and brain tumor. Parents should be reassured that the condition is benign, and that the attacks will cease in a few months or years.", "contents": "Benign paroxysmal vertigo of childhood. Thirty-three cases of benign paroxysmal vertigo in childhood have been seen at our institution since the disorder was recognized ten years ago. Progression from paroxysmal torticollis of infancy to paroxysmal vertigo of childhood is documented. Ear infections and allergy appeared causative in a few, but not most, of the cases. The most important consideration for the pediatrician is to rule out epilepsy and brain tumor. Parents should be reassured that the condition is benign, and that the attacks will cease in a few months or years.", "PMID": 973613}
{"id": "pubmed23n0032_9390", "title": "Juvenile diabetes mellitus and serum lipids and lipoprotein levels.", "content": "Cholesterol, triglyceride, and lipoprotein levels were determined in serum from 40 children with diabetes and from controls. Mean cholesterol levels in the children with diabetes (205 +/- 78 mg/dl) were statisically higher than for controls (155 +/- 27 mg/dl), as were mean triglyceride levels (120 +/- 63 vs 85 +/- 23 mg/dl). Eight of the children with diabetes had hypercholesterolemia, five had hypertriglyceridemia, and nine had combined hypercholesterolemia and hypertriglyceridemia. Low-density lipoprotein levels were statistically higher and high-density lipoprotein levels statistically lower for children with diabetes compared with control children. Increased urine glucose spillage  was found to correlate with higher serum triglyceride levels, suggesting that the elevated triglyceride levels may have been related to diabetes control. With the known association between hyperlipidemia and coronary heart disease (CHD) and between diabetes and CHD, the results of the present study indicate that all children with juvenile diabetes mellitus should have a serum lipid analysis annually.", "contents": "Juvenile diabetes mellitus and serum lipids and lipoprotein levels. Cholesterol, triglyceride, and lipoprotein levels were determined in serum from 40 children with diabetes and from controls. Mean cholesterol levels in the children with diabetes (205 +/- 78 mg/dl) were statisically higher than for controls (155 +/- 27 mg/dl), as were mean triglyceride levels (120 +/- 63 vs 85 +/- 23 mg/dl). Eight of the children with diabetes had hypercholesterolemia, five had hypertriglyceridemia, and nine had combined hypercholesterolemia and hypertriglyceridemia. Low-density lipoprotein levels were statistically higher and high-density lipoprotein levels statistically lower for children with diabetes compared with control children. Increased urine glucose spillage  was found to correlate with higher serum triglyceride levels, suggesting that the elevated triglyceride levels may have been related to diabetes control. With the known association between hyperlipidemia and coronary heart disease (CHD) and between diabetes and CHD, the results of the present study indicate that all children with juvenile diabetes mellitus should have a serum lipid analysis annually.", "PMID": 973614}
{"id": "pubmed23n0032_9391", "title": "Gelastic Epilepsy. Onset in neonatal period.", "content": "The phenomenon of gelastic epilepsy was first described in 1873, yet fewer than 100 patients with this disorder have been reported on to date. The purpose of this article is to report on the first two patients to our knowledge with the onset of these seizures in the immediate neonatal period. Both patients have been shown to have posterior hypothalamic mass lesions presumably of congenital origin, and have remained free of neurologic progression of the disease with conservative treatment.", "contents": "Gelastic Epilepsy. Onset in neonatal period. The phenomenon of gelastic epilepsy was first described in 1873, yet fewer than 100 patients with this disorder have been reported on to date. The purpose of this article is to report on the first two patients to our knowledge with the onset of these seizures in the immediate neonatal period. Both patients have been shown to have posterior hypothalamic mass lesions presumably of congenital origin, and have remained free of neurologic progression of the disease with conservative treatment.", "PMID": 973617}
{"id": "pubmed23n0032_9392", "title": "Primary leiomyosarcoma of the lung in childhood.", "content": "Primary leiomyosarcomas of the lung and trachea are rare tumors. Only five cases have been reported in children (two of a lung, one of a mainstem bronchus, one of the carina, and one of the trachea). We report a new case in a 14-month-old boy and review the previous cases.", "contents": "Primary leiomyosarcoma of the lung in childhood. Primary leiomyosarcomas of the lung and trachea are rare tumors. Only five cases have been reported in children (two of a lung, one of a mainstem bronchus, one of the carina, and one of the trachea). We report a new case in a 14-month-old boy and review the previous cases.", "PMID": 973618}
{"id": "pubmed23n0032_9393", "title": "Eosinophilic infiltration of the gastrointestinal tract in children.", "content": "Three children had eosinophilic infiltration of the gastrointestinal tract. This entity has two forms--diffuse and circumscribed. The diffuse lesion is confined mainly to the gastric antrum. Surgical resection of this form is seldom necessary. Involvement of the appendix with this form has not been previously described. The polypoid lesion, which may arise in any segment of the gastrointestinal tract, commonly produces secondary gastrointestinal symptoms and is amenable to local surgical excision.", "contents": "Eosinophilic infiltration of the gastrointestinal tract in children. Three children had eosinophilic infiltration of the gastrointestinal tract. This entity has two forms--diffuse and circumscribed. The diffuse lesion is confined mainly to the gastric antrum. Surgical resection of this form is seldom necessary. Involvement of the appendix with this form has not been previously described. The polypoid lesion, which may arise in any segment of the gastrointestinal tract, commonly produces secondary gastrointestinal symptoms and is amenable to local surgical excision.", "PMID": 973619}
{"id": "pubmed23n0032_9394", "title": "Hypokalemic crisis simulating intestinal obstruction in a 4-year-old girl. A consequence of 17alpha-hydroxylase deficiency.", "content": "A 4-year-old girl had abdominal distention, muscular weakness, renal tubular dysfunction, and hypertension associated with hypokalemic metabolic alkalosis. There were no clinical symptoms of cortisol deficiency, but there was excessive deoxycorticosterone and cortisocsterone production. Basal plasma aldosterone levels were undetectable; however, adrenocorticotropic hormone (ACTH) stimulation brought plasma aldosterone levels up to normal. The urinary pregnanediol, tetrahydro-deoxycorticosterone (THDOC), and tetrahydrocorticosterone (THB) concentrations were elevated. Stimulation of ACTH failed to increase urinary 17-ketosteroid, 17-hydroxycorticosteroid, or plasma cortisol levels significantly, while urinary THDOC, THB, and plasma corticosterone concentrations were further elevated. The elevated plasma corticosteroid intermediates were suppressed by dexamethasone administration. When physiologic doses of dexamethasone were administered, the hypertension, electrolyte imbalance, and abnormal corticosteroid secretion were all corrected. The studies indicated a partial 17alpha-hydroxylase defect in this patient.", "contents": "Hypokalemic crisis simulating intestinal obstruction in a 4-year-old girl. A consequence of 17alpha-hydroxylase deficiency. A 4-year-old girl had abdominal distention, muscular weakness, renal tubular dysfunction, and hypertension associated with hypokalemic metabolic alkalosis. There were no clinical symptoms of cortisol deficiency, but there was excessive deoxycorticosterone and cortisocsterone production. Basal plasma aldosterone levels were undetectable; however, adrenocorticotropic hormone (ACTH) stimulation brought plasma aldosterone levels up to normal. The urinary pregnanediol, tetrahydro-deoxycorticosterone (THDOC), and tetrahydrocorticosterone (THB) concentrations were elevated. Stimulation of ACTH failed to increase urinary 17-ketosteroid, 17-hydroxycorticosteroid, or plasma cortisol levels significantly, while urinary THDOC, THB, and plasma corticosterone concentrations were further elevated. The elevated plasma corticosteroid intermediates were suppressed by dexamethasone administration. When physiologic doses of dexamethasone were administered, the hypertension, electrolyte imbalance, and abnormal corticosteroid secretion were all corrected. The studies indicated a partial 17alpha-hydroxylase defect in this patient.", "PMID": 973620}
{"id": "pubmed23n0032_9395", "title": "Relapsing polychondritis and retroperitoneal fibrosis in an 8-year-old boy.", "content": "An 8-year-old boy with symptoms of relapsing polychondritis for five years developed retroperitoneal fibrosis and ureteral obstruction. In addition to involvement of the cartilage of his joints, ear, and nose, there was significant arterial involvement. The patient had a very good response to adrenal corticosteroid therapy. After one year of follow-up, he has had a definite increase in joint mobility and has normal renal function.", "contents": "Relapsing polychondritis and retroperitoneal fibrosis in an 8-year-old boy. An 8-year-old boy with symptoms of relapsing polychondritis for five years developed retroperitoneal fibrosis and ureteral obstruction. In addition to involvement of the cartilage of his joints, ear, and nose, there was significant arterial involvement. The patient had a very good response to adrenal corticosteroid therapy. After one year of follow-up, he has had a definite increase in joint mobility and has normal renal function.", "PMID": 973621}
{"id": "pubmed23n0032_9396", "title": "Diagnosis of death in comatose patients under resuscitation treatment: a critical review of the Harvard report.", "content": "In this Article, which draws primarily on continental West European views on death and dying, the author contends that the Harvard criteria for irreversible coma (1968) are not reliable for diagnosing death in comatose patients under resuscitation treatment. The Article suggests that use of the Harvard Criteria to diagnose death leaves such patients legally unprotected against surgical assaults such as organ removal and biomedical experiments while they still may be living and capable of perception, possibly including the perception of pain and the spoken word. An alternative to the Harvard Criteria--angiography--is offered, and several additional issues related to the definition and diagnosis of death are discussed. Finally, the author suggests that even prior to death, termination of resuscitation treatment of irreversibly comatose patients, though followed by death, should be lawful.", "contents": "Diagnosis of death in comatose patients under resuscitation treatment: a critical review of the Harvard report. In this Article, which draws primarily on continental West European views on death and dying, the author contends that the Harvard criteria for irreversible coma (1968) are not reliable for diagnosing death in comatose patients under resuscitation treatment. The Article suggests that use of the Harvard Criteria to diagnose death leaves such patients legally unprotected against surgical assaults such as organ removal and biomedical experiments while they still may be living and capable of perception, possibly including the perception of pain and the spoken word. An alternative to the Harvard Criteria--angiography--is offered, and several additional issues related to the definition and diagnosis of death are discussed. Finally, the author suggests that even prior to death, termination of resuscitation treatment of irreversibly comatose patients, though followed by death, should be lawful.", "PMID": 973624}
{"id": "pubmed23n0032_9397", "title": "Clinical approaches to teaching legal medicine to physicians: medicolegal emergencies and consultations.", "content": "Physicians-in-training generally have insufficient exposure to legal medicine to prepare them for the medicolegal problems they will encounter in practice. This Article outlines the current structure of the field of legal medicine in America, and sets forth two strategies with clinical orientations for teaching legal medicine to physicians-in-training: (1) providing a didactic course offering accurate and practical information on medicolegal emergency situations; and (2) establishing a consultation service in legal medicine capable of providing clinical information or training in such settings as the emergency room, the outpatient departments, and the inpatient wards.", "contents": "Clinical approaches to teaching legal medicine to physicians: medicolegal emergencies and consultations. Physicians-in-training generally have insufficient exposure to legal medicine to prepare them for the medicolegal problems they will encounter in practice. This Article outlines the current structure of the field of legal medicine in America, and sets forth two strategies with clinical orientations for teaching legal medicine to physicians-in-training: (1) providing a didactic course offering accurate and practical information on medicolegal emergency situations; and (2) establishing a consultation service in legal medicine capable of providing clinical information or training in such settings as the emergency room, the outpatient departments, and the inpatient wards.", "PMID": 973626}
{"id": "pubmed23n0032_9398", "title": "State legislation on abortion after Roe v. Wade: selected constitutional issues.", "content": "Over the past three years, a great volume of legislation on abortion has been produced by state legislatures in an attempt to fill the vacuum created by the United States Supreme Court's 1973 decision in Roe v. Wade. This Article examines several of the most common types of statutory provisions and assesses their constitutionality in light of Roe v. Wade and other applicable federal and state legal standards.", "contents": "State legislation on abortion after Roe v. Wade: selected constitutional issues. Over the past three years, a great volume of legislation on abortion has been produced by state legislatures in an attempt to fill the vacuum created by the United States Supreme Court's 1973 decision in Roe v. Wade. This Article examines several of the most common types of statutory provisions and assesses their constitutionality in light of Roe v. Wade and other applicable federal and state legal standards.", "PMID": 973625}
{"id": "pubmed23n0032_9399", "title": "Compatibility of antineoplastic, antibiotic and corticosteroid drugs in intravenous admixtures.", "content": "Through the use of absorption spectroscopy and visual observations, the compatibility of selected oncologic, antibiotic and corticosteroid drugs in intravenous admixtures was determined. The six drugs used in this study were methotrexate sodium, prednisolone sodium phosphate, sodium cephalothin, 5-fluorouracil, cytarabine and vincristine sulfate. These were cross-matched in pairs, using 5% dextrose injection as the vehicle. By obtaining the ultraviolet absorption spectrum of each of the drugs alone in the 5% dextrose injection, reference of standard spectra were obtained which could be used as a comparison for the spectra of the drugs in admixture. This comparison permitted detection of any alterations in the spectrum which would suggest chemical (nonvisual) incompatibility. Of the 13 combinations examined, four pairs appeared to be chemically incompatible. These were: 5-fluorouracil and methotrexate sodium; 5-fluorouracil and cytarabine; prednisolone sodium phosphate and methotrexate sodium; and methotrexate sodium and cytarabine.", "contents": "Compatibility of antineoplastic, antibiotic and corticosteroid drugs in intravenous admixtures. Through the use of absorption spectroscopy and visual observations, the compatibility of selected oncologic, antibiotic and corticosteroid drugs in intravenous admixtures was determined. The six drugs used in this study were methotrexate sodium, prednisolone sodium phosphate, sodium cephalothin, 5-fluorouracil, cytarabine and vincristine sulfate. These were cross-matched in pairs, using 5% dextrose injection as the vehicle. By obtaining the ultraviolet absorption spectrum of each of the drugs alone in the 5% dextrose injection, reference of standard spectra were obtained which could be used as a comparison for the spectra of the drugs in admixture. This comparison permitted detection of any alterations in the spectrum which would suggest chemical (nonvisual) incompatibility. Of the 13 combinations examined, four pairs appeared to be chemically incompatible. These were: 5-fluorouracil and methotrexate sodium; 5-fluorouracil and cytarabine; prednisolone sodium phosphate and methotrexate sodium; and methotrexate sodium and cytarabine.", "PMID": 973628}
{"id": "pubmed23n0032_9400", "title": "Improved taste acceptability for an oral hyperalimentation dosage form.", "content": "Various flavoring agents were evaluated as to their ability to improve the taste acceptability of Vivovnex-HN, a potent oral hyperalimentation diet. Several imitation flavors were incorporated into the Vivonex-HN powder in different concentrations with and without an artificial sweetener. The mixtures were rated by a taste panel. Among the unsweetened flavors, spray-dried orange juice, Sealva imitation mint No.381 and cherry mint imitation appeared to be the most acceptable flavors. Among the individual flavors sweetened with 0.025% sodium saccharin, rum, strawberry and raspberry proved to be the most acceptable. Among all of the formulations studied, a combination of rum, strawberry and raspberry with 0.025% sodium saccharin was found to be optimal.", "contents": "Improved taste acceptability for an oral hyperalimentation dosage form. Various flavoring agents were evaluated as to their ability to improve the taste acceptability of Vivovnex-HN, a potent oral hyperalimentation diet. Several imitation flavors were incorporated into the Vivonex-HN powder in different concentrations with and without an artificial sweetener. The mixtures were rated by a taste panel. Among the unsweetened flavors, spray-dried orange juice, Sealva imitation mint No.381 and cherry mint imitation appeared to be the most acceptable flavors. Among the individual flavors sweetened with 0.025% sodium saccharin, rum, strawberry and raspberry proved to be the most acceptable. Among all of the formulations studied, a combination of rum, strawberry and raspberry with 0.025% sodium saccharin was found to be optimal.", "PMID": 973629}
{"id": "pubmed23n0032_9401", "title": "Pharmacy-based special infant formula service.", "content": "The preparation of infant formulas by a hospital pharmacy for a 280-bed department of pediatrics is discussed. A formulary of acceptable special therapeutic formulas was developed in conjunction with the pediatricians. Space and equipment originally intended for intravenous admixture and total parenteral nutrition services were supplemented to include special infant formula services. The personnel and equipment requirements, and quality control procedures, of the service are discussed.", "contents": "Pharmacy-based special infant formula service. The preparation of infant formulas by a hospital pharmacy for a 280-bed department of pediatrics is discussed. A formulary of acceptable special therapeutic formulas was developed in conjunction with the pediatricians. Space and equipment originally intended for intravenous admixture and total parenteral nutrition services were supplemented to include special infant formula services. The personnel and equipment requirements, and quality control procedures, of the service are discussed.", "PMID": 973630}
{"id": "pubmed23n0032_9402", "title": "Organizing patient data for use in clinical pharmacy practice and education.", "content": "A simple, low-cost manual data retrieval system, which  provides selective access to all patient cases monitored by pharmacists, is described. The system uses notched cards which are coded by clinical descriptors. The codes are based on a modification of the International Calssification of Diseases, Adapted, disease-diagnosis coding system. This multiple entry system is flexible and can be easily converted to a computer-based system.", "contents": "Organizing patient data for use in clinical pharmacy practice and education. A simple, low-cost manual data retrieval system, which  provides selective access to all patient cases monitored by pharmacists, is described. The system uses notched cards which are coded by clinical descriptors. The codes are based on a modification of the International Calssification of Diseases, Adapted, disease-diagnosis coding system. This multiple entry system is flexible and can be easily converted to a computer-based system.", "PMID": 973631}
{"id": "pubmed23n0032_9403", "title": "A drug experience registry.", "content": "The Drug Exerience Registry is a system for the recording and filing of drug-related patient events in a readily retrievable form. The purpose of the registry is to accumulate information on adverse drug reactions, unusual therapeutic successes of failures, and other experiences that may be useful for education, research or promotion of rational drug therapy. Data are recorded on case report forms which are categorized by organ-disease system affected, the clinical event and involved drug(s). These are then filed in the clinical event file along with literature synopses that serve as guides in evaluating the incidents and determining proper therapy. A drug file serves as a cross index to the clinical event file and allows one to quickly locate clinical events involving a drug. Both files contain sufficient hospital data to allow later retrieval of additional information if needed.", "contents": "A drug experience registry. The Drug Exerience Registry is a system for the recording and filing of drug-related patient events in a readily retrievable form. The purpose of the registry is to accumulate information on adverse drug reactions, unusual therapeutic successes of failures, and other experiences that may be useful for education, research or promotion of rational drug therapy. Data are recorded on case report forms which are categorized by organ-disease system affected, the clinical event and involved drug(s). These are then filed in the clinical event file along with literature synopses that serve as guides in evaluating the incidents and determining proper therapy. A drug file serves as a cross index to the clinical event file and allows one to quickly locate clinical events involving a drug. Both files contain sufficient hospital data to allow later retrieval of additional information if needed.", "PMID": 973632}
{"id": "pubmed23n0032_9404", "title": "Computerized medication monitoring system.", "content": "A computerized medication monitoring system for alerting and warning of potential adverse drug reactions is described. The system integrates computerized data on each hospital patient (medications, clinical laboratory, blood gas, ECG, ALLERGIES, DIAGNOSIs, etc.) and returns to the pharmacist warning messages and suggestions regarding patient drug therapy. The broad data base allows for nearly complete drug therapy monitoring. When a warning message is received, the pharmacist contacts the physician or nursing staff and explains the potential problem. The system also generates prescription labels and patient drug profiles which are used in a unit dose dispensing system. Five percent of 13,727 patients monitored have had drug alerts. Of these alterts, 44.9% were drug-laboratory contraindications and only 28.9% were drug-drug interactions. Of the 690 alerts received, 77% resulted in the physician changing the patient's therapy.", "contents": "Computerized medication monitoring system. A computerized medication monitoring system for alerting and warning of potential adverse drug reactions is described. The system integrates computerized data on each hospital patient (medications, clinical laboratory, blood gas, ECG, ALLERGIES, DIAGNOSIs, etc.) and returns to the pharmacist warning messages and suggestions regarding patient drug therapy. The broad data base allows for nearly complete drug therapy monitoring. When a warning message is received, the pharmacist contacts the physician or nursing staff and explains the potential problem. The system also generates prescription labels and patient drug profiles which are used in a unit dose dispensing system. Five percent of 13,727 patients monitored have had drug alerts. Of these alterts, 44.9% were drug-laboratory contraindications and only 28.9% were drug-drug interactions. Of the 690 alerts received, 77% resulted in the physician changing the patient's therapy.", "PMID": 973633}
{"id": "pubmed23n0032_9405", "title": "Comparison of stability-indicating assay methods: ultraviolet, high pressure liquid chromatography and acid dye.", "content": "Three techniques--ultraviolet, high pressure liquid chromatography (HPLC) and acid dye--were compared as stability-indicating assay methods. Investigations on six drugs (codeine phosphate, ephedrine hydrochloride, morphine sulfate, procaine hydrochloride, pyrilamine maleate and thiamine hydrochloride) indicate that HPLC is the most reliable method.", "contents": "Comparison of stability-indicating assay methods: ultraviolet, high pressure liquid chromatography and acid dye. Three techniques--ultraviolet, high pressure liquid chromatography (HPLC) and acid dye--were compared as stability-indicating assay methods. Investigations on six drugs (codeine phosphate, ephedrine hydrochloride, morphine sulfate, procaine hydrochloride, pyrilamine maleate and thiamine hydrochloride) indicate that HPLC is the most reliable method.", "PMID": 973634}
{"id": "pubmed23n0032_9406", "title": "Iron poisoning: report of a fatal case in an adult.", "content": "A fatal case of iron poisoning in a suicide attempt is reported, and the toxicity and pathophysiology of iron are discussed. The four phases of the clinical features of iron poisoning are presented, and the general treatment of iron toxicity is discussed.", "contents": "Iron poisoning: report of a fatal case in an adult. A fatal case of iron poisoning in a suicide attempt is reported, and the toxicity and pathophysiology of iron are discussed. The four phases of the clinical features of iron poisoning are presented, and the general treatment of iron toxicity is discussed.", "PMID": 973635}
{"id": "pubmed23n0032_9407", "title": "The short- and long-term prognosis of patients with transmural and nontransmural myocardial infarction.", "content": "To compare the long-term prognosis in patients surviving transmural with patients surviving nontransmural myocardial infarctions, the records of 188 consecutive patients with clinical histories and enzyme elevations consistent with acute infarction were reviewed. According to standard electrocardiographic criteria the patients were divided into two groups: 148 with transmural myocardial infarction (group 1) and 40 with nontransmural myocardial infarction (group 2). Of the patients who survived hospitalization, follow-up data were obtained on 119 of 124 patients in group 1 and 36 of 37 patients in group 2 at a mean follow-up period of 36 months. In group 2, the patients had a high incidence of sudden death after discharge (33 per cent in group 2 versus 15 per cent in group 1, p less than 0.02) as well as a significantly higher incidence of death from all cardiac causes (41.6 per cent in group 2 versus 24.3 per cent in group 1, p less than 0.05). Furthermore, the patients in group 2 still alive at the end of the follow-up period had an increased incidence of angina pectoris and of recurrent infarction. The data suggest that patients with nontransmural myocardial infarction carry a particularly guarded prognosis.", "contents": "The short- and long-term prognosis of patients with transmural and nontransmural myocardial infarction. To compare the long-term prognosis in patients surviving transmural with patients surviving nontransmural myocardial infarctions, the records of 188 consecutive patients with clinical histories and enzyme elevations consistent with acute infarction were reviewed. According to standard electrocardiographic criteria the patients were divided into two groups: 148 with transmural myocardial infarction (group 1) and 40 with nontransmural myocardial infarction (group 2). Of the patients who survived hospitalization, follow-up data were obtained on 119 of 124 patients in group 1 and 36 of 37 patients in group 2 at a mean follow-up period of 36 months. In group 2, the patients had a high incidence of sudden death after discharge (33 per cent in group 2 versus 15 per cent in group 1, p less than 0.02) as well as a significantly higher incidence of death from all cardiac causes (41.6 per cent in group 2 versus 24.3 per cent in group 1, p less than 0.05). Furthermore, the patients in group 2 still alive at the end of the follow-up period had an increased incidence of angina pectoris and of recurrent infarction. The data suggest that patients with nontransmural myocardial infarction carry a particularly guarded prognosis.", "PMID": 973641}
{"id": "pubmed23n0032_9408", "title": "Effects of alternating phosphorus and calcium infusions on osteoporosis.", "content": "The osteoblastic effect of inorganic phosphorus and the inhibitory action of calcium on parathyroid hormone secretion formed the basis for a therapeutic trial in which both elements were given intravenously in an alternating sequence for one year to five patients with severe osteoporosis. During treatment, calcium and phosphorus balances were positive, and serum phosphorus concentrations decreased. Iliac crest bone biopsy specimens obtained one year after beginning treatment demonstrated an increase in the thickness of cortical bone. All patients experienced lasting relief of bone pain, and the rate of spontaneous fractures decreased from at least one fracture per year to none following the beginning of treatment. These findings suggest that long-term treatment with alternating phosphorus and calcium infusions may result in lasting relief of symptomatic osteoporosis. It is proposed that the observed improvement reflects an increase in bone mass resulting from enhanced bone formation.", "contents": "Effects of alternating phosphorus and calcium infusions on osteoporosis. The osteoblastic effect of inorganic phosphorus and the inhibitory action of calcium on parathyroid hormone secretion formed the basis for a therapeutic trial in which both elements were given intravenously in an alternating sequence for one year to five patients with severe osteoporosis. During treatment, calcium and phosphorus balances were positive, and serum phosphorus concentrations decreased. Iliac crest bone biopsy specimens obtained one year after beginning treatment demonstrated an increase in the thickness of cortical bone. All patients experienced lasting relief of bone pain, and the rate of spontaneous fractures decreased from at least one fracture per year to none following the beginning of treatment. These findings suggest that long-term treatment with alternating phosphorus and calcium infusions may result in lasting relief of symptomatic osteoporosis. It is proposed that the observed improvement reflects an increase in bone mass resulting from enhanced bone formation.", "PMID": 973642}
{"id": "pubmed23n0032_9409", "title": "Steroid myopathy in connective tissue disease.", "content": "In eight women with polymyositis (three patients), systemic lupus erythematosus (SLE) (three patients), rheumatoid arthritis (one patient) and shoulder-hand syndrome (one patient), weakness developed during high dose prednisone therapy. These women were studied using serial functional and manual muscle tests, determination of serum glutamic oxaloacetic transminase (SGOT), creatine phosphokinase (CPK) and serum aldolase levels, and urinary excretion of creatine. Insidious onset of weakness was characteristic. Myalgias were seen in five patients and unusual sudden weakness in two. Weakness was always most severe in the pelvic girdle muscles; there was a lesser involvement of shoulder girdle and distal muscles. Serum muscle enzyme levels were normal in all cases, but urinary creatine excretion was invariably increased and proved to be the most sensitive laboratory indicator for clinical diagnosis and for monitoring patient improvement. Serial urinary creatine excretion and serum enzyme studies were of value in differenting steroid myopathy from a flare of myositis in patients with connective tissue disease. Diagnosis and effective management were achieved by the use of readily available laboratory and clinical procedures without resorting to muscle biopsy.", "contents": "Steroid myopathy in connective tissue disease. In eight women with polymyositis (three patients), systemic lupus erythematosus (SLE) (three patients), rheumatoid arthritis (one patient) and shoulder-hand syndrome (one patient), weakness developed during high dose prednisone therapy. These women were studied using serial functional and manual muscle tests, determination of serum glutamic oxaloacetic transminase (SGOT), creatine phosphokinase (CPK) and serum aldolase levels, and urinary excretion of creatine. Insidious onset of weakness was characteristic. Myalgias were seen in five patients and unusual sudden weakness in two. Weakness was always most severe in the pelvic girdle muscles; there was a lesser involvement of shoulder girdle and distal muscles. Serum muscle enzyme levels were normal in all cases, but urinary creatine excretion was invariably increased and proved to be the most sensitive laboratory indicator for clinical diagnosis and for monitoring patient improvement. Serial urinary creatine excretion and serum enzyme studies were of value in differenting steroid myopathy from a flare of myositis in patients with connective tissue disease. Diagnosis and effective management were achieved by the use of readily available laboratory and clinical procedures without resorting to muscle biopsy.", "PMID": 973643}
{"id": "pubmed23n0032_9410", "title": "The course of pregnancy in patients with artificial heart valves.", "content": "Twenty-five women with prosthetic heart valves (PHV) became pregnant 28 times. Twenty-six of the pregnancies occurred while the patients were receiving oral anticoagulants and these were continued throughout in 25 pregnancies. Dipyridamole or dipyridamole and acetylsalicyclic acid were used in 22 pregnancies. Eighteen infants were delivered, one with a congenital corneal leukoma; none had hemorrhagic complications; their psychomotor development was normal. Nine women aborted 10 times, including patients with two PHV, pelvic trauma and self-induced abortion. We could not detect excess anticoagulation in eight of the nine who had spontaneous losses; excess anticoagulation occurred five weeks before an abortion. There were no maternal deaths despite numerous complications; in two women, brain embolism was related to short interruptions of anticoagulation. The details of management are mentioned. We lack enough evidence to suggest routine sterilization, routine interruption of coumarin therapy during pregnancy or routine interruption of pregnancy in women with certain types and models of PHV; however, pregnancy under such conditions, plus antithrombotic therapy, carries a high risk for the product and a potential teratogenic effect. Women with one or two PHV can have children if their management is closely supervised and if extreme care is taken with the use of oral anticoagulants.", "contents": "The course of pregnancy in patients with artificial heart valves. Twenty-five women with prosthetic heart valves (PHV) became pregnant 28 times. Twenty-six of the pregnancies occurred while the patients were receiving oral anticoagulants and these were continued throughout in 25 pregnancies. Dipyridamole or dipyridamole and acetylsalicyclic acid were used in 22 pregnancies. Eighteen infants were delivered, one with a congenital corneal leukoma; none had hemorrhagic complications; their psychomotor development was normal. Nine women aborted 10 times, including patients with two PHV, pelvic trauma and self-induced abortion. We could not detect excess anticoagulation in eight of the nine who had spontaneous losses; excess anticoagulation occurred five weeks before an abortion. There were no maternal deaths despite numerous complications; in two women, brain embolism was related to short interruptions of anticoagulation. The details of management are mentioned. We lack enough evidence to suggest routine sterilization, routine interruption of coumarin therapy during pregnancy or routine interruption of pregnancy in women with certain types and models of PHV; however, pregnancy under such conditions, plus antithrombotic therapy, carries a high risk for the product and a potential teratogenic effect. Women with one or two PHV can have children if their management is closely supervised and if extreme care is taken with the use of oral anticoagulants.", "PMID": 973644}
{"id": "pubmed23n0032_9411", "title": "Splenic abscess. Report of 10 cases and review of the literature.", "content": "Ten cases of splenic abscess seen between 1964 and 1974 are reviewed. Pain referable to the abscess was the most common symptom, but was present in only five cases. Fever was present in all but one case. Tenderness in the region of the spleen was noted in six cases, in three cases the spleen was palpable and in one case a large mass in the upper left quadrant of the abdomen was palpated. Abdominal films were suggestive of the diagnosis in two cases, and the liver-spleen scan demonstrated a defect in three cases. Seven abscesses were caused by gram-negative bacilli of bowel origin; the etiologic agents in the other three were Staphylococcus aureus, Streptobacillus moniliformis and a Nocardia species. Associated conditions predisposing to splenic abscess included trauma in three cases, splenic arteritis or embolization in five cases, and foci of infection elsewhere in the body in six, including two cases of endocarditis. The mortality was 60 per cent. Half of the deaths were due to the underlying illness, but failure to diagnose splenic abscess contributed to a fatal outcome in three cases.", "contents": "Splenic abscess. Report of 10 cases and review of the literature. Ten cases of splenic abscess seen between 1964 and 1974 are reviewed. Pain referable to the abscess was the most common symptom, but was present in only five cases. Fever was present in all but one case. Tenderness in the region of the spleen was noted in six cases, in three cases the spleen was palpable and in one case a large mass in the upper left quadrant of the abdomen was palpated. Abdominal films were suggestive of the diagnosis in two cases, and the liver-spleen scan demonstrated a defect in three cases. Seven abscesses were caused by gram-negative bacilli of bowel origin; the etiologic agents in the other three were Staphylococcus aureus, Streptobacillus moniliformis and a Nocardia species. Associated conditions predisposing to splenic abscess included trauma in three cases, splenic arteritis or embolization in five cases, and foci of infection elsewhere in the body in six, including two cases of endocarditis. The mortality was 60 per cent. Half of the deaths were due to the underlying illness, but failure to diagnose splenic abscess contributed to a fatal outcome in three cases.", "PMID": 973645}
{"id": "pubmed23n0032_9412", "title": "Unusual electrocardiographic finding--bifascicular block due to hyperkalemia.", "content": "A unique case in which the patient had bifascicular block consisting of right bundle branch block and left posterior hemiblock as a result of marked hyperkalemia is presented. To our knowledge, this is the first reported case in which such unusual electrocardiographic abnormalities due to hyperkalemia were demonstrated. The electrocardiographic abnormalities produced by hyperkalemia in this case disappeared promptly by hemodialysis, as the serum potassium level returned to normal. It has been stressed that hyperkalemia should be considered as an important etiologic factor in the differential diagnosis of bundle branch block, hemiblocks and bifascicular block, particularly when these intraventricular blocks are produced suddenly.", "contents": "Unusual electrocardiographic finding--bifascicular block due to hyperkalemia. A unique case in which the patient had bifascicular block consisting of right bundle branch block and left posterior hemiblock as a result of marked hyperkalemia is presented. To our knowledge, this is the first reported case in which such unusual electrocardiographic abnormalities due to hyperkalemia were demonstrated. The electrocardiographic abnormalities produced by hyperkalemia in this case disappeared promptly by hemodialysis, as the serum potassium level returned to normal. It has been stressed that hyperkalemia should be considered as an important etiologic factor in the differential diagnosis of bundle branch block, hemiblocks and bifascicular block, particularly when these intraventricular blocks are produced suddenly.", "PMID": 973647}
{"id": "pubmed23n0032_9413", "title": "Bilateral scalp necrosis in temporal arteritis. A rare complication of Horton's disease.", "content": "In an elderly man bilateral temporal scalp necrosis complicating temporal (systemic giant cell) arteritis developed. The patient also demonstrated other features characteristic of this entity: (1) exquisite scalp tenderness and headache, (2) onset late in life, (3) visual disturbances, (4) general debility, (5) diagnostic temporal artery biopsy, and (6) gradual healing with glucocorticosteroid therapy. A computer assisted review revealed 13 similar cases of scalp necrosis associated with temporal arteritis published in the English literature.", "contents": "Bilateral scalp necrosis in temporal arteritis. A rare complication of Horton's disease. In an elderly man bilateral temporal scalp necrosis complicating temporal (systemic giant cell) arteritis developed. The patient also demonstrated other features characteristic of this entity: (1) exquisite scalp tenderness and headache, (2) onset late in life, (3) visual disturbances, (4) general debility, (5) diagnostic temporal artery biopsy, and (6) gradual healing with glucocorticosteroid therapy. A computer assisted review revealed 13 similar cases of scalp necrosis associated with temporal arteritis published in the English literature.", "PMID": 973648}
{"id": "pubmed23n0032_9414", "title": "Immunoblastic lymphadenopathy. Evolution into a malignant lymphoma with plasmacytoid features.", "content": "In the patient described progressive lymphadenopathy, splenomegaly and interstitial pulmonary disease developed two months after the development of immunoblastic lymphadenopathy. Light microscopic examination of a lymph node biopsy specimen suggested a diagnosis of immunoblastic sarcoma. Evolution of this malignant lymphoma into a leukemic phase allowed detailed studies of the malignant cells by electron microscopy, cytochemical staining and immunologic technics. Evidence is presented that this is a case of malignant lymphoma with plasmacytoid and not lymphoid features. Review of the literature on immunoblastic sarcoma suggests that light microscopy and clinical setting are not sufficient to define a homogeneous clinicopathologic disease.", "contents": "Immunoblastic lymphadenopathy. Evolution into a malignant lymphoma with plasmacytoid features. In the patient described progressive lymphadenopathy, splenomegaly and interstitial pulmonary disease developed two months after the development of immunoblastic lymphadenopathy. Light microscopic examination of a lymph node biopsy specimen suggested a diagnosis of immunoblastic sarcoma. Evolution of this malignant lymphoma into a leukemic phase allowed detailed studies of the malignant cells by electron microscopy, cytochemical staining and immunologic technics. Evidence is presented that this is a case of malignant lymphoma with plasmacytoid and not lymphoid features. Review of the literature on immunoblastic sarcoma suggests that light microscopy and clinical setting are not sufficient to define a homogeneous clinicopathologic disease.", "PMID": 973649}
{"id": "pubmed23n0032_9415", "title": "C1q deficiency associated with urticarial-like lesions and cutaneous vasculitis.", "content": "We describe a 46 year old women with a seven year history of urticarial-like symptoms and cutaneous vasculitis with marked deficiency of C1q in the presence of normal levels of C1r and C1s and high titers of low molecular weight (7S) C1q precipitins. Hemolytic C1 activity, which was greatly reduced, was restored upon the addition of purified C1q. The other complement components were present in moderately reduced or normal levels. This patient bears resemblance to several other persons previously described with urticarial-like lesions in association with selective deficiency of C1q. The similarity of the clinical features, pathologic diagnosis, C1q levels disproportionately deficient in relation to other complement components and low molecular weight C1q precipitins support the conclusion that these are causally related in a symptom complex. The underlying basis is yet to be defined.", "contents": "C1q deficiency associated with urticarial-like lesions and cutaneous vasculitis. We describe a 46 year old women with a seven year history of urticarial-like symptoms and cutaneous vasculitis with marked deficiency of C1q in the presence of normal levels of C1r and C1s and high titers of low molecular weight (7S) C1q precipitins. Hemolytic C1 activity, which was greatly reduced, was restored upon the addition of purified C1q. The other complement components were present in moderately reduced or normal levels. This patient bears resemblance to several other persons previously described with urticarial-like lesions in association with selective deficiency of C1q. The similarity of the clinical features, pathologic diagnosis, C1q levels disproportionately deficient in relation to other complement components and low molecular weight C1q precipitins support the conclusion that these are causally related in a symptom complex. The underlying basis is yet to be defined.", "PMID": 973650}
{"id": "pubmed23n0032_9416", "title": "A complement independent erythropoietic inhibitor acting on the progenitor cell in refractory anemia.", "content": "An erythropoietic inhibitor was detected in the serum of a patient with refractory anemia. Using an in vitro heme synthesis method, the patient's serum produced tenfold inhibition of erythropoietin-stimulated radioactive iron (Fe59) incorporation into heme of normal human marrow at 72 hours, as compared with AB serum. In a separate experiment the patient's serum produced threefold inhibition, whereas immunoglobulin G (IgG) prepared from the same serum sample produced 12-fold inhibition. To identify the site of action of the inhibitor, serum was tested in a cell culture system whereby human marrow cells, grown in a plasma clot, respond to exogenous erythropoietin with the appearance of nucleated erythroid colonies. Each colony arises from a committed erythroid progenitor. The patient's serum produced a two- or tenfold reduction in the number of colonies from normal human marrow. The effect was also demonstrated on autologous marrow obtained when the patient was in \"partial clinical remission\". Serum samples obtained at various times during the course of the patient's illness all demonstrated a suppressive effect on colony growth. All serums were heat-inactivated, and total hemolytic complement could not be detected in either culture system. It is concluded that the anemia is due to an inhibitor, probably of IgG class, that acts on the erythroid progenitor cell. The absence of heat-labile complement components in the culture systems suggests that the mechanism is not due to immune cytolysis.", "contents": "A complement independent erythropoietic inhibitor acting on the progenitor cell in refractory anemia. An erythropoietic inhibitor was detected in the serum of a patient with refractory anemia. Using an in vitro heme synthesis method, the patient's serum produced tenfold inhibition of erythropoietin-stimulated radioactive iron (Fe59) incorporation into heme of normal human marrow at 72 hours, as compared with AB serum. In a separate experiment the patient's serum produced threefold inhibition, whereas immunoglobulin G (IgG) prepared from the same serum sample produced 12-fold inhibition. To identify the site of action of the inhibitor, serum was tested in a cell culture system whereby human marrow cells, grown in a plasma clot, respond to exogenous erythropoietin with the appearance of nucleated erythroid colonies. Each colony arises from a committed erythroid progenitor. The patient's serum produced a two- or tenfold reduction in the number of colonies from normal human marrow. The effect was also demonstrated on autologous marrow obtained when the patient was in \"partial clinical remission\". Serum samples obtained at various times during the course of the patient's illness all demonstrated a suppressive effect on colony growth. All serums were heat-inactivated, and total hemolytic complement could not be detected in either culture system. It is concluded that the anemia is due to an inhibitor, probably of IgG class, that acts on the erythroid progenitor cell. The absence of heat-labile complement components in the culture systems suggests that the mechanism is not due to immune cytolysis.", "PMID": 973651}
{"id": "pubmed23n0032_9417", "title": "Hypertension, hyperreninemia and a solitary renal cyst in an adolescent.", "content": "Solitary renal cysts associated with hypertension and documented hyperreninemia are relatively uncommon. A 13 year old boy with these findings is described (pressure 160/120 mm Hg). Contrast studies of the urinary tract and arteriography with selective renal vein sampling demonstrated a solitary cyst in the right kidney. The ratio of plasma renin activity in the right renal vein to left renal vein (RRV:LRV) was 2.35:1. After drainage of the cyst, there was a marked reduction in both systolic and diastolic pressures (126/84 mm Hg). Hypertension has not recurred during 14 months' follow-up.", "contents": "Hypertension, hyperreninemia and a solitary renal cyst in an adolescent. Solitary renal cysts associated with hypertension and documented hyperreninemia are relatively uncommon. A 13 year old boy with these findings is described (pressure 160/120 mm Hg). Contrast studies of the urinary tract and arteriography with selective renal vein sampling demonstrated a solitary cyst in the right kidney. The ratio of plasma renin activity in the right renal vein to left renal vein (RRV:LRV) was 2.35:1. After drainage of the cyst, there was a marked reduction in both systolic and diastolic pressures (126/84 mm Hg). Hypertension has not recurred during 14 months' follow-up.", "PMID": 973652}
{"id": "pubmed23n0032_9418", "title": "The angiographic appearance of benign renal oxyphilic adenoma.", "content": "Four cases of oxyphilic cortical renal adenomas identified by angiography are described. A suggestive angiographic appearance is outlined for adenomas limited to the cortex: avascularity or hypovascularity; renal vessels splayed and stretched around tumor base; faint parenchymal blush during nephrograpm phase; and tumor well marginated, appearing to arise from the renal cortex. Masses that fulfill the above criteria are adequately treated with excisional biopsy if frozen section confirms their benignity.", "contents": "The angiographic appearance of benign renal oxyphilic adenoma. Four cases of oxyphilic cortical renal adenomas identified by angiography are described. A suggestive angiographic appearance is outlined for adenomas limited to the cortex: avascularity or hypovascularity; renal vessels splayed and stretched around tumor base; faint parenchymal blush during nephrograpm phase; and tumor well marginated, appearing to arise from the renal cortex. Masses that fulfill the above criteria are adequately treated with excisional biopsy if frozen section confirms their benignity.", "PMID": 973655}
{"id": "pubmed23n0032_9419", "title": "Asymmetry of the pelvic ureters in normal females.", "content": "A total of 65 cases showing asymmetry of the pelvic ureters of greater than 1.4 cm for which no cause could be identified are presented. Only one was a male. The right ureter was situated more medially in 61 of the 64 females. Comparison of the characteristics of these patients with 51 females with symmetrical pelvic ureters revealed no differences except for a significantly more frequent left-sided position of the uterus in the asymmetrical group. Gross medial displacement of the right ureter in a female probably represents a normal variant in a large majority of patients.", "contents": "Asymmetry of the pelvic ureters in normal females. A total of 65 cases showing asymmetry of the pelvic ureters of greater than 1.4 cm for which no cause could be identified are presented. Only one was a male. The right ureter was situated more medially in 61 of the 64 females. Comparison of the characteristics of these patients with 51 females with symmetrical pelvic ureters revealed no differences except for a significantly more frequent left-sided position of the uterus in the asymmetrical group. Gross medial displacement of the right ureter in a female probably represents a normal variant in a large majority of patients.", "PMID": 973656}
{"id": "pubmed23n0032_9420", "title": "The radiologic manifestations of idiopathic intestinal pseudoobstruction.", "content": "Six patients with idopathic intestinal pseudoobstruction underwent extensive radiographic evaluation of the gastrointestinal tract. Propulsive motor activity was consistently absent. All had smooth muscle dysfunction of the esophagus, small bowel, and colon, and two had abnormal gastric emptying. Two forms of the syndrome were observed, characterized by either hyper- or hypoactive smooth muscle. In the hyperactive form chaotic, spontaneous contractions of the esophagus and small intestine occurred and extensive diverticular disease of the colon was present. In the hypoactive form the esophagus was atonic and there was marked widening and hypomotility of the small intestine and colon. The presence of two forms of smooth muscle dysfunction suggests that the syndrome has a heterogeneous pathology and pathophysiology.", "contents": "The radiologic manifestations of idiopathic intestinal pseudoobstruction. Six patients with idopathic intestinal pseudoobstruction underwent extensive radiographic evaluation of the gastrointestinal tract. Propulsive motor activity was consistently absent. All had smooth muscle dysfunction of the esophagus, small bowel, and colon, and two had abnormal gastric emptying. Two forms of the syndrome were observed, characterized by either hyper- or hypoactive smooth muscle. In the hyperactive form chaotic, spontaneous contractions of the esophagus and small intestine occurred and extensive diverticular disease of the colon was present. In the hypoactive form the esophagus was atonic and there was marked widening and hypomotility of the small intestine and colon. The presence of two forms of smooth muscle dysfunction suggests that the syndrome has a heterogeneous pathology and pathophysiology.", "PMID": 973657}
{"id": "pubmed23n0032_9421", "title": "Radiographic evaluation of complications after jejunoileal bypass surgery.", "content": "A retrospective analysis of 89 patients who underwent jejunoileal bypass surgery for morbid obesity disclosed 33 complications that were detected radiographically. Intestinal obstruction (10.1% of patients), cholecystitis (5.6%), renal stones (4.5%), peptic ulcer (3.4%), megacolon (6.7%), and elongation of the small intestine with hypertrophy of the mucosal folds of the jejunum (6.7%) were diagnosed solely by radiographic means.", "contents": "Radiographic evaluation of complications after jejunoileal bypass surgery. A retrospective analysis of 89 patients who underwent jejunoileal bypass surgery for morbid obesity disclosed 33 complications that were detected radiographically. Intestinal obstruction (10.1% of patients), cholecystitis (5.6%), renal stones (4.5%), peptic ulcer (3.4%), megacolon (6.7%), and elongation of the small intestine with hypertrophy of the mucosal folds of the jejunum (6.7%) were diagnosed solely by radiographic means.", "PMID": 973658}
{"id": "pubmed23n0032_9422", "title": "The small bowel enema: experience with 150 examination.", "content": "Clinical application of the small bowel enema has received waxing and waning enthusiasm since it was first described in 1929. Our experiences with 150 small bowel enema studies is presented. The Sellink method was used for patients without ileostomy, (140), and retrograde studies for those with ileostomy. The merits of the small bowel enema compared to the conventional technique are discussed. The small bowel enema is the preferred method for radiologic assessment of the small bowel in patients whose clinical picture suggests small bowel disease.", "contents": "The small bowel enema: experience with 150 examination. Clinical application of the small bowel enema has received waxing and waning enthusiasm since it was first described in 1929. Our experiences with 150 small bowel enema studies is presented. The Sellink method was used for patients without ileostomy, (140), and retrograde studies for those with ileostomy. The merits of the small bowel enema compared to the conventional technique are discussed. The small bowel enema is the preferred method for radiologic assessment of the small bowel in patients whose clinical picture suggests small bowel disease.", "PMID": 973659}
{"id": "pubmed23n0032_9423", "title": "Pneumoperitoneum: importance of right upper quadrant features.", "content": "Supine abdominal radiographs of 59 patients with documented pneumoperitoneum were evaluated for findings of pneumoperitoneum. The commonly described signs were seen in only 17%. Localized intraperitoneal air in the right upper quadrant was the most frequent finding, seen in 49% of patients. Identification of these collections of air is dependent upon knowledge of the anatomy of the perihepatic spaces.", "contents": "Pneumoperitoneum: importance of right upper quadrant features. Supine abdominal radiographs of 59 patients with documented pneumoperitoneum were evaluated for findings of pneumoperitoneum. The commonly described signs were seen in only 17%. Localized intraperitoneal air in the right upper quadrant was the most frequent finding, seen in 49% of patients. Identification of these collections of air is dependent upon knowledge of the anatomy of the perihepatic spaces.", "PMID": 973660}
{"id": "pubmed23n0032_9424", "title": "Necrotizing enterocolitis after open heart surgery employing hypothermia and cardiopulmonary bypass.", "content": "Three patients, 1-11 months of age, who underwent open heart surgery with cardiopulmonary bypass and profound hypothermia, developed necrotizing enterocolitis postoperatively leading to death. Pneumatosis intestinalis and portal vein gas were demonstrated radiographically. Necrotizing enterocolitis has not been previously reported as a complication of cardiopulmonary bypass and profound hypothermia.", "contents": "Necrotizing enterocolitis after open heart surgery employing hypothermia and cardiopulmonary bypass. Three patients, 1-11 months of age, who underwent open heart surgery with cardiopulmonary bypass and profound hypothermia, developed necrotizing enterocolitis postoperatively leading to death. Pneumatosis intestinalis and portal vein gas were demonstrated radiographically. Necrotizing enterocolitis has not been previously reported as a complication of cardiopulmonary bypass and profound hypothermia.", "PMID": 973661}
{"id": "pubmed23n0032_9425", "title": "Radiology in primary hyperaldosteronism.", "content": "Autonomous hypersecretion of aldosterone (primary hyperaldosteronism) is caused by either hyperplasia (usually bilateral) or an adenoma (frequently unilateral) of the adrenal cortex. Systemic hypertension due to an aldosteronoma is a potentially curable condition through surgical extirpation of the offending organ. In our experience with 37 patients clinically suspected to have primary hyperaldosteronism, radiological methods contributed significantly in preoperative diagnosis. These included (1) selective bilateral adrenal vein catheterization and blood sample collection, (2) adrenal venography, and (3) radioisotope adrenal scan. Unilateral hyperfunction could be accurately detected by the aldosterone assays from the collected samples. When adrenal venography was technically satisfactory, a nodule or aggregate of nodules measuring at least 7 mm and located on the margin of the gland or 1.5 cm or more in diameter when located in the center of the gland were readily identified. Enlarged adrenal gland on venography, in itself, was not a dependable index of a hyperfunctioning gland. Presence of a higher uptake on one side on the radioisotope adrenal scan did not always indicate the hyperfunctioning gland, but lack of lateralization of adrenal hyperfunction was more accurately predicted on the radioisotope scan than by venography. Four histopathological patterns were recognized in the surgically removed adrenal glands, but no correlation between these patterns and clinical behavior or postoperative course was found.", "contents": "Radiology in primary hyperaldosteronism. Autonomous hypersecretion of aldosterone (primary hyperaldosteronism) is caused by either hyperplasia (usually bilateral) or an adenoma (frequently unilateral) of the adrenal cortex. Systemic hypertension due to an aldosteronoma is a potentially curable condition through surgical extirpation of the offending organ. In our experience with 37 patients clinically suspected to have primary hyperaldosteronism, radiological methods contributed significantly in preoperative diagnosis. These included (1) selective bilateral adrenal vein catheterization and blood sample collection, (2) adrenal venography, and (3) radioisotope adrenal scan. Unilateral hyperfunction could be accurately detected by the aldosterone assays from the collected samples. When adrenal venography was technically satisfactory, a nodule or aggregate of nodules measuring at least 7 mm and located on the margin of the gland or 1.5 cm or more in diameter when located in the center of the gland were readily identified. Enlarged adrenal gland on venography, in itself, was not a dependable index of a hyperfunctioning gland. Presence of a higher uptake on one side on the radioisotope adrenal scan did not always indicate the hyperfunctioning gland, but lack of lateralization of adrenal hyperfunction was more accurately predicted on the radioisotope scan than by venography. Four histopathological patterns were recognized in the surgically removed adrenal glands, but no correlation between these patterns and clinical behavior or postoperative course was found.", "PMID": 973662}
{"id": "pubmed23n0032_9426", "title": "Angiographic manifestations of ruputured abdominal aortic aneurysm.", "content": "Abdominal angiography was performed in five cases of ruptured abdominal aortic aneurysms. In four the diagnosis of a ruptured aneurysm was not suspected clinically. Angiographic findings included (1) an irregular collection of contrast outside the opacified aortic lumen or calcified aneurysm which persisted after contrast cleared from the aorta, (2) localized saccular dilatation of the aorta in which contrast remained after being cleared from the aorta, and (3) an aortocaval fistula.", "contents": "Angiographic manifestations of ruputured abdominal aortic aneurysm. Abdominal angiography was performed in five cases of ruptured abdominal aortic aneurysms. In four the diagnosis of a ruptured aneurysm was not suspected clinically. Angiographic findings included (1) an irregular collection of contrast outside the opacified aortic lumen or calcified aneurysm which persisted after contrast cleared from the aorta, (2) localized saccular dilatation of the aorta in which contrast remained after being cleared from the aorta, and (3) an aortocaval fistula.", "PMID": 973663}
{"id": "pubmed23n0032_9427", "title": "Posttraumatic osteolysis of the distal clavicle with emphasis on early radiologic changes.", "content": "Erosion of progressive resorption of the distal clavicle following trauma has been described but merits greater attention due to its frequency. Six cases of posttraumatic osteolysis of the distal clavicle are reported with emphasis on early radiographic detection. Soft tissue swelling, deminerlization, and loss of the subarticular cortex at the injured clavicular tip antecede the more commonly recognized gross erosion. Recognition of this early stage, with consequent immobilization, may shorten the course of this process and decrease the prolonged disability. The pathogenesis of the process is discussed and illustrated.", "contents": "Posttraumatic osteolysis of the distal clavicle with emphasis on early radiologic changes. Erosion of progressive resorption of the distal clavicle following trauma has been described but merits greater attention due to its frequency. Six cases of posttraumatic osteolysis of the distal clavicle are reported with emphasis on early radiographic detection. Soft tissue swelling, deminerlization, and loss of the subarticular cortex at the injured clavicular tip antecede the more commonly recognized gross erosion. Recognition of this early stage, with consequent immobilization, may shorten the course of this process and decrease the prolonged disability. The pathogenesis of the process is discussed and illustrated.", "PMID": 973664}
{"id": "pubmed23n0032_9428", "title": "Radiographic findings in early acquired syphilis: case report and cirtical review.", "content": "The radiographic and pathophysiologic features of early acquired syphilis are discussed. Bone changes occur in early acquired syphilis and should not be confused with gummas of late syphilis. The radiographic findings are protean and may exist without a clinical history of a cutaneous lesion. The skull, clavicle, and tibia are the sites most frequently involved.", "contents": "Radiographic findings in early acquired syphilis: case report and cirtical review. The radiographic and pathophysiologic features of early acquired syphilis are discussed. Bone changes occur in early acquired syphilis and should not be confused with gummas of late syphilis. The radiographic findings are protean and may exist without a clinical history of a cutaneous lesion. The skull, clavicle, and tibia are the sites most frequently involved.", "PMID": 973665}
{"id": "pubmed23n0032_9429", "title": "Cancer in the \"cold\" breast thermogram.", "content": "The hallmark of the normal breast thermogram is relative symmetry of vascular configuration and thermal content with preservation of the breast contour. Accepted criteria of abnormality are predicated upon graphic and thermal asymmetry with emphasis placed upon elevated temperature, an increase in the number of discernible vessels, and distorted vascular patterns. The association of a confirmed breast cancer and an avascular thermogram has been labeled a false negative. Avascularity (\"cold\" breast), particularly in the lower half, with normal vessels in the same location of the opposite breast is suggested as an additional characteristic of abnormality. Illustrative cases are presented.", "contents": "Cancer in the \"cold\" breast thermogram. The hallmark of the normal breast thermogram is relative symmetry of vascular configuration and thermal content with preservation of the breast contour. Accepted criteria of abnormality are predicated upon graphic and thermal asymmetry with emphasis placed upon elevated temperature, an increase in the number of discernible vessels, and distorted vascular patterns. The association of a confirmed breast cancer and an avascular thermogram has been labeled a false negative. Avascularity (\"cold\" breast), particularly in the lower half, with normal vessels in the same location of the opposite breast is suggested as an additional characteristic of abnormality. Illustrative cases are presented.", "PMID": 973666}
{"id": "pubmed23n0032_9430", "title": "Chordoma: radiologic evaluation of 20 cases.", "content": "Experience with 20 cases of chordoma is summarized. Three of four intracranial tumors presented as sellar tumors. The clivus remained intact for 2, 3 1/2, and 4 years, respectively, in three patients despite extensive destructive lesions of the sellar region. Our data and those of others indicate that approximately half the patients with chordomas have erosion of the clivus at the time of initial medical evaluation. The other half mainly have sellar destructive lesions with or without associated clival erosion. Calcification occurs in 50%-70% of intracranial chordomas. A nasopharyngeal mass is present in one-third. Vertebral chordomas often involve two or more vertebrae, producing destructive lesions which often have a sclerotic rim. The intervertebral discs are commonly affected. A paraspinal soft tissue mass, half the time containing calcification, is often present. Sacral lesions are characterized by a midline destructive lesion, occasionally associated with expansion, with or without osteosclerosis. A firm, fixed, extrarectal, presacral mass which may contain calcification or fragments of bone is almost always present.", "contents": "Chordoma: radiologic evaluation of 20 cases. Experience with 20 cases of chordoma is summarized. Three of four intracranial tumors presented as sellar tumors. The clivus remained intact for 2, 3 1/2, and 4 years, respectively, in three patients despite extensive destructive lesions of the sellar region. Our data and those of others indicate that approximately half the patients with chordomas have erosion of the clivus at the time of initial medical evaluation. The other half mainly have sellar destructive lesions with or without associated clival erosion. Calcification occurs in 50%-70% of intracranial chordomas. A nasopharyngeal mass is present in one-third. Vertebral chordomas often involve two or more vertebrae, producing destructive lesions which often have a sclerotic rim. The intervertebral discs are commonly affected. A paraspinal soft tissue mass, half the time containing calcification, is often present. Sacral lesions are characterized by a midline destructive lesion, occasionally associated with expansion, with or without osteosclerosis. A firm, fixed, extrarectal, presacral mass which may contain calcification or fragments of bone is almost always present.", "PMID": 973667}
{"id": "pubmed23n0032_9431", "title": "The value of consultation among radiologists.", "content": "The value of group consultation in improving the accuracy of radiologic diagnosis was assessed. Abnormalities on 24 films were pointed out to six groups of three radiologists each. They were asked to reach a specific diagnosis for each case. Accuracy was compared in three settingss: (1) without consultation; (2) with consultation leading to a diagnosis by group consensus; and (3) with consultation followed by independent diagnoses. The accuracy of over 75% of the radiologist improved after consultation in either setting. The extent of improvement was inversely related to the initial accuracy level of the individual. When two or three radiologists agreed initially, they were more frequently correct than not. Group discussion followed by individual diagnosis is a practical approach to radiologic consultation.", "contents": "The value of consultation among radiologists. The value of group consultation in improving the accuracy of radiologic diagnosis was assessed. Abnormalities on 24 films were pointed out to six groups of three radiologists each. They were asked to reach a specific diagnosis for each case. Accuracy was compared in three settingss: (1) without consultation; (2) with consultation leading to a diagnosis by group consensus; and (3) with consultation followed by independent diagnoses. The accuracy of over 75% of the radiologist improved after consultation in either setting. The extent of improvement was inversely related to the initial accuracy level of the individual. When two or three radiologists agreed initially, they were more frequently correct than not. Group discussion followed by individual diagnosis is a practical approach to radiologic consultation.", "PMID": 973668}
{"id": "pubmed23n0032_9432", "title": "RAPORT radiology system: results of clinical trials.", "content": "RAPORTTM, an automated radiology management and reporting system is currently operational in more than 25 hospital departments of varying sizes and types. Data have been gathered from 19 installations in operation for 6 months or longer and four systems which failed. Findings were as follows: 1. Mark sense forms were used in 71% of cases (29% dictation) and for an average of 70% of all pathologic material. 2. Report turnaround time ranged from 0.1 to 6 hr (average 2.5 hr) depending on departmental priorties and organization. 3. Rejection of the system was based upon inadequate documentation of needs prior to installation, poor preparation of staff members, and underestimation of the impact on computer systems on existing organizations. 4. System downtime averaged 2.5 hr per month. 5. The ratio of pathologic to normal reports did not vary significantly among university, government, and private hospitals. 6. RAPORT can no longer be considered an experimental system. Properly managed, it is an effective administrative tool which can significantly improve departmental service.", "contents": "RAPORT radiology system: results of clinical trials. RAPORTTM, an automated radiology management and reporting system is currently operational in more than 25 hospital departments of varying sizes and types. Data have been gathered from 19 installations in operation for 6 months or longer and four systems which failed. Findings were as follows: 1. Mark sense forms were used in 71% of cases (29% dictation) and for an average of 70% of all pathologic material. 2. Report turnaround time ranged from 0.1 to 6 hr (average 2.5 hr) depending on departmental priorties and organization. 3. Rejection of the system was based upon inadequate documentation of needs prior to installation, poor preparation of staff members, and underestimation of the impact on computer systems on existing organizations. 4. System downtime averaged 2.5 hr per month. 5. The ratio of pathologic to normal reports did not vary significantly among university, government, and private hospitals. 6. RAPORT can no longer be considered an experimental system. Properly managed, it is an effective administrative tool which can significantly improve departmental service.", "PMID": 973669}
{"id": "pubmed23n0032_9433", "title": "Gray scale cholecystosonography: an evaluation of accuracy.", "content": "The accuracy of gray scale cholecystosonography is evaluated by comparing oral cholecystography and ultrasonography findings on 141 patients. Confirmation of findings by oral cholecystography or surgery was available in 123, and there was agreement by cholecystosonography in 111, for an accuracy of 90%. Of 39 patients with gallstones, 30 were correctly diagnosed by ultrasound. These results represent an improvement in the accuracy of gray scale ultrasonography for evaluation of the gallbladder compared to previous reports and demonstrate the potential usefulness of the procedure for evaluation of the nonvisualized gallbladder.", "contents": "Gray scale cholecystosonography: an evaluation of accuracy. The accuracy of gray scale cholecystosonography is evaluated by comparing oral cholecystography and ultrasonography findings on 141 patients. Confirmation of findings by oral cholecystography or surgery was available in 123, and there was agreement by cholecystosonography in 111, for an accuracy of 90%. Of 39 patients with gallstones, 30 were correctly diagnosed by ultrasound. These results represent an improvement in the accuracy of gray scale ultrasonography for evaluation of the gallbladder compared to previous reports and demonstrate the potential usefulness of the procedure for evaluation of the nonvisualized gallbladder.", "PMID": 973670}
{"id": "pubmed23n0032_9434", "title": "The dependence of radiographic mottle on beam quality.", "content": "The dependence of radiographic mottle on beam quality has been studied for a variety of film/screen combinations. The measurement technique consisted of scanning the radiographs with a microdenitometer; the analogue signal from the microdesitometer was gated and recorded with a multichannel analyzer. The sampling aperture was 500 mum in diameter and was chosen because its spatial frequency response approximates that of the eye. The standard deviation of the density fluctuations was calculated directly from the number versus density spectrum accumulated in the analyzer. The range of standard deviations' for the various combinations and x-ray tube kilovoltages studied from approximately 0.009 to approximately 0.021 density units. For commonly used combinations, very little kilovoltage dependence was observed. The relative amount varied for different screen phosphors and is attributed to differences in the absorption characteristics.", "contents": "The dependence of radiographic mottle on beam quality. The dependence of radiographic mottle on beam quality has been studied for a variety of film/screen combinations. The measurement technique consisted of scanning the radiographs with a microdenitometer; the analogue signal from the microdesitometer was gated and recorded with a multichannel analyzer. The sampling aperture was 500 mum in diameter and was chosen because its spatial frequency response approximates that of the eye. The standard deviation of the density fluctuations was calculated directly from the number versus density spectrum accumulated in the analyzer. The range of standard deviations' for the various combinations and x-ray tube kilovoltages studied from approximately 0.009 to approximately 0.021 density units. For commonly used combinations, very little kilovoltage dependence was observed. The relative amount varied for different screen phosphors and is attributed to differences in the absorption characteristics.", "PMID": 973671}
{"id": "pubmed23n0032_9435", "title": "An evaluation of procedures used in staging carcinoma of the cervix.", "content": "A review of 227 cases of invasive carcinoma of the cervix was undertaken to determine the efficacy of procedures using in the staging of this disease. All patients had a pretreatment chest radiography and intravenous pyelogram. 96.5% had pretreatment cystoscopy, 98.6% had pretreatment proctoscopy, and 92% had a pretreatment barium enema. These patients were retrospectively staged on the findings of physical examination only. Each additional procedure was then evaluated by comparison with the initial staging. Cystoscopy and chest film findings each would have changed the clinical stage in fewer than 1% of cases, barium enema in 1.4%, and proctoscopy in 2.2%. No patient had a positive barium enema without a positive proctoscopy, while two patients had positive proctoscopies with negative barium enemas. The overall yield of positive findings by pretreatment intravenous pyelography was 7.3%. Lymphangiography, although inconsistently done, yielded positive findings in all stages. Chest radiographs, intravenous pyelography, proctoscopy, and lymphangiography are recommended as part of the pretreatment workup of patients with carcinoma of the cervix. Routine barium enema is no longer recommended, and cystoscopy is only recommended in patients with clinical stage IIB disease or greater.", "contents": "An evaluation of procedures used in staging carcinoma of the cervix. A review of 227 cases of invasive carcinoma of the cervix was undertaken to determine the efficacy of procedures using in the staging of this disease. All patients had a pretreatment chest radiography and intravenous pyelogram. 96.5% had pretreatment cystoscopy, 98.6% had pretreatment proctoscopy, and 92% had a pretreatment barium enema. These patients were retrospectively staged on the findings of physical examination only. Each additional procedure was then evaluated by comparison with the initial staging. Cystoscopy and chest film findings each would have changed the clinical stage in fewer than 1% of cases, barium enema in 1.4%, and proctoscopy in 2.2%. No patient had a positive barium enema without a positive proctoscopy, while two patients had positive proctoscopies with negative barium enemas. The overall yield of positive findings by pretreatment intravenous pyelography was 7.3%. Lymphangiography, although inconsistently done, yielded positive findings in all stages. Chest radiographs, intravenous pyelography, proctoscopy, and lymphangiography are recommended as part of the pretreatment workup of patients with carcinoma of the cervix. Routine barium enema is no longer recommended, and cystoscopy is only recommended in patients with clinical stage IIB disease or greater.", "PMID": 973672}
{"id": "pubmed23n0032_9436", "title": "Results of involved field and extended field radiotherapy in patients with pathologic state I and II Hodgkin's disease.", "content": "A series of 44 previously untreated patients was found to have stage I or II Hodgkin's disease on the basis of a diagnostic workup which included laparotomy and splenectomy in 39 cases. The patients were randomized to either involved field or extended field radiotherapy. No difference was found in overall survival rates between the two groups. Regional recurrences were seen in four patients in the involved field group and in none of the extended field group. Regional recurrences were seen in four patients in the involved field group and in none of the extended field group. Radiotherapy to the extended field treatment port (generally an extended mantle) can be given with acceptable side effects. Staging laparotomy was of definite value, changing the classification and resulting therapy of two groups: (1) patients with clinical stage (CS) I or II, pathologic stage (PS) III involvement, who were treated with appropriately larger radiotherapy fields; and (2) patients with CS III, PS I or II, who were spared unnecessarily extensive radiotherapy.", "contents": "Results of involved field and extended field radiotherapy in patients with pathologic state I and II Hodgkin's disease. A series of 44 previously untreated patients was found to have stage I or II Hodgkin's disease on the basis of a diagnostic workup which included laparotomy and splenectomy in 39 cases. The patients were randomized to either involved field or extended field radiotherapy. No difference was found in overall survival rates between the two groups. Regional recurrences were seen in four patients in the involved field group and in none of the extended field group. Regional recurrences were seen in four patients in the involved field group and in none of the extended field group. Radiotherapy to the extended field treatment port (generally an extended mantle) can be given with acceptable side effects. Staging laparotomy was of definite value, changing the classification and resulting therapy of two groups: (1) patients with clinical stage (CS) I or II, pathologic stage (PS) III involvement, who were treated with appropriately larger radiotherapy fields; and (2) patients with CS III, PS I or II, who were spared unnecessarily extensive radiotherapy.", "PMID": 973673}
{"id": "pubmed23n0032_9437", "title": "Successful transcatheter embolic control of massive hematobilia secondary to liver biopsy.", "content": "A 43-year-old female alcoholic had massive hematobilia secondary to arterial trauma of a liver biopsy. The arterial bleeding site was successfully embolized through an angiographic catheter with Gelfoam sponge. No liver ischemia occurred and the patient has remained well.", "contents": "Successful transcatheter embolic control of massive hematobilia secondary to liver biopsy. A 43-year-old female alcoholic had massive hematobilia secondary to arterial trauma of a liver biopsy. The arterial bleeding site was successfully embolized through an angiographic catheter with Gelfoam sponge. No liver ischemia occurred and the patient has remained well.", "PMID": 973674}
{"id": "pubmed23n0032_9438", "title": "Treatment of symptomatic hepatic cyst by percutaneous instillation of Pantopaque.", "content": "A large, symptomatic, simple hepatic cysts was managed by the percutaneous instillation of Pantopaque. The method was based on reports of similar percutaneous treatment of renal cysts. In 6 months the patient was asymptomatic and radiographs demonstrated evidence of a fibrous reaction within the cyst. The patient remains asymptomatic 16 months after puncture. These results suggest that percutaneous instillation of sclerosing agents may provide an alternative to operative procedures.", "contents": "Treatment of symptomatic hepatic cyst by percutaneous instillation of Pantopaque. A large, symptomatic, simple hepatic cysts was managed by the percutaneous instillation of Pantopaque. The method was based on reports of similar percutaneous treatment of renal cysts. In 6 months the patient was asymptomatic and radiographs demonstrated evidence of a fibrous reaction within the cyst. The patient remains asymptomatic 16 months after puncture. These results suggest that percutaneous instillation of sclerosing agents may provide an alternative to operative procedures.", "PMID": 973675}
{"id": "pubmed23n0032_9439", "title": "The limbus vertebra: an anterior disc herniation demonstrated by discography.", "content": "Limbus vertebra results from an intrabody herniation of disc material. It can be mistaken for a fracture, infection, or tumor, resulting in unnecessary invasive diagnostic procedures. A case is presented in which the lesion was diagnosed by discography. Most radiologists are unfamiliar with discograms, yet they are useful because they opacify the anteriorly herniated portion of the nucleus pulposus.", "contents": "The limbus vertebra: an anterior disc herniation demonstrated by discography. Limbus vertebra results from an intrabody herniation of disc material. It can be mistaken for a fracture, infection, or tumor, resulting in unnecessary invasive diagnostic procedures. A case is presented in which the lesion was diagnosed by discography. Most radiologists are unfamiliar with discograms, yet they are useful because they opacify the anteriorly herniated portion of the nucleus pulposus.", "PMID": 973676}
{"id": "pubmed23n0032_9440", "title": "Patellar giant cell tumor.", "content": "Primary patellar neoplasms are distinctly unusual. A rare case of patellar giant cell tumor which caused clinical, radiographic, and histologic confusion is described. The lesion progressed rapidly, underscoring the potential aggressive behavior of the tumor. Amputation is the preferred treatment. A review of the literature is also presented.", "contents": "Patellar giant cell tumor. Primary patellar neoplasms are distinctly unusual. A rare case of patellar giant cell tumor which caused clinical, radiographic, and histologic confusion is described. The lesion progressed rapidly, underscoring the potential aggressive behavior of the tumor. Amputation is the preferred treatment. A review of the literature is also presented.", "PMID": 973677}
{"id": "pubmed23n0032_9441", "title": "Esophageal aperistalsis secondary to metastatic invasion of the myenteric plexus.", "content": "A case of aperistalsis of the esophagus secondary to isolated invasion of the myenteric plexus of Auerbach by metastatic carcinoma is presented. The unique pattern of tumor involvement and the resultant aperistalsis supports the contention that involvement of the neurons in the deep myenteric plexus, either by inflammation or tumor, may be the key factor in producing aperistalsis. No similar cases were found in the literature.", "contents": "Esophageal aperistalsis secondary to metastatic invasion of the myenteric plexus. A case of aperistalsis of the esophagus secondary to isolated invasion of the myenteric plexus of Auerbach by metastatic carcinoma is presented. The unique pattern of tumor involvement and the resultant aperistalsis supports the contention that involvement of the neurons in the deep myenteric plexus, either by inflammation or tumor, may be the key factor in producing aperistalsis. No similar cases were found in the literature.", "PMID": 973678}
{"id": "pubmed23n0032_9442", "title": "Salicylate-induced pulmonary edema.", "content": "A case of pulmonary edema secondary to salicylate intoxication is described. The pulmonary wedge pressure was normal, excluding cardiogenic pulmonary edema. Thus salicylate intoxication should be considered as a rare cause in the differential diagnosis of pulmonary edema with a normal heart size. The pulmonary edema resolved gradually over 8 days. Literature relative to salicylate-induced pulmonary edema is reviewed.", "contents": "Salicylate-induced pulmonary edema. A case of pulmonary edema secondary to salicylate intoxication is described. The pulmonary wedge pressure was normal, excluding cardiogenic pulmonary edema. Thus salicylate intoxication should be considered as a rare cause in the differential diagnosis of pulmonary edema with a normal heart size. The pulmonary edema resolved gradually over 8 days. Literature relative to salicylate-induced pulmonary edema is reviewed.", "PMID": 973679}
{"id": "pubmed23n0032_9443", "title": "Depression of hypoxic ventilatory response by nitrous oxide.", "content": "Ventilatory responses to CO2 and hypoxia were measured in four normal volunteers breathing 30-50 per cent N2O  with and without added inspiratory resistance. CO2 response was measured by a steady-state technique, hypoxic response by a non-steady-state progressive technique. Added inspiratory resistance depressed ventilatory responses to both CO2 and hypoxia. N2O had no effect on CO2 response either with or without resistance. N2O depressed the ventilatory response to hypoxia without added resistance and further depressed the response measured with added resistance. It is thought that this was probably the result of selective depression of peripheral chemoceptor function by N2O.", "contents": "Depression of hypoxic ventilatory response by nitrous oxide. Ventilatory responses to CO2 and hypoxia were measured in four normal volunteers breathing 30-50 per cent N2O  with and without added inspiratory resistance. CO2 response was measured by a steady-state technique, hypoxic response by a non-steady-state progressive technique. Added inspiratory resistance depressed ventilatory responses to both CO2 and hypoxia. N2O had no effect on CO2 response either with or without resistance. N2O depressed the ventilatory response to hypoxia without added resistance and further depressed the response measured with added resistance. It is thought that this was probably the result of selective depression of peripheral chemoceptor function by N2O.", "PMID": 973689}
{"id": "pubmed23n0032_9444", "title": "Internal carotid artery stump pressure and cerebral blood flow during carotid endarterectomy: modification by halothane, enflurane, and innovar.", "content": "Carotid endarterectomy requires temporary surgical occlusion of the involved carotid artery. During occlusion, the minimally acceptable (critical) internal carotid artery stump pressure is reported to be 50 torr, whereas for regional cerebral blood flow (rCBF), a critical range is reported to be 18-24 ml/100 g/min. During 90 carotid endarterectomies, rCBF and stump pressure were measured and the EEG continuously monitored. A positive correlation between rCBF and stump pressure (i.e., when both were either above or below their respective critical values) was observed in only 58 per cent of the cases. In 28 per cent stump pressures of less than 50 torr were observed despite rCBF's above 24 ml/100 g/min and normal EEG's. In 8 per cent stump pressures were more than 50 torr but rCBF's were less than 18 ml/100 g/min and EEG changes of ischemia were commonly observed. In the remaining 6 per cent rCBF's were marginal (18-24 ml/100 g/min) while stump pressures were more than 50 torr and EEG changes were not observed. The relationship between stump pressure and rCBF was influenced by the anesthetic. In the absence of transient ischemia during occlusion (that is, rCBF greater than 18 ml/100 g/min), halothane and enflurane anesthesia were associated with significantly higher rCBF's and lower stump pressures than was neuroleptanesthesia. Pre-occlusion and post-occlusion rCBF measurements also demonstrated cerebral vasodilation by halothane and enflurane (halothane greater than enflurane) and vasoconstriction by neuroleptanesthesia. It is concluded that stump pressure is an unreliable index of CBF during carotid occlusion and that its relationship to CBF is considerably influenced by the anesthetic.", "contents": "Internal carotid artery stump pressure and cerebral blood flow during carotid endarterectomy: modification by halothane, enflurane, and innovar. Carotid endarterectomy requires temporary surgical occlusion of the involved carotid artery. During occlusion, the minimally acceptable (critical) internal carotid artery stump pressure is reported to be 50 torr, whereas for regional cerebral blood flow (rCBF), a critical range is reported to be 18-24 ml/100 g/min. During 90 carotid endarterectomies, rCBF and stump pressure were measured and the EEG continuously monitored. A positive correlation between rCBF and stump pressure (i.e., when both were either above or below their respective critical values) was observed in only 58 per cent of the cases. In 28 per cent stump pressures of less than 50 torr were observed despite rCBF's above 24 ml/100 g/min and normal EEG's. In 8 per cent stump pressures were more than 50 torr but rCBF's were less than 18 ml/100 g/min and EEG changes of ischemia were commonly observed. In the remaining 6 per cent rCBF's were marginal (18-24 ml/100 g/min) while stump pressures were more than 50 torr and EEG changes were not observed. The relationship between stump pressure and rCBF was influenced by the anesthetic. In the absence of transient ischemia during occlusion (that is, rCBF greater than 18 ml/100 g/min), halothane and enflurane anesthesia were associated with significantly higher rCBF's and lower stump pressures than was neuroleptanesthesia. Pre-occlusion and post-occlusion rCBF measurements also demonstrated cerebral vasodilation by halothane and enflurane (halothane greater than enflurane) and vasoconstriction by neuroleptanesthesia. It is concluded that stump pressure is an unreliable index of CBF during carotid occlusion and that its relationship to CBF is considerably influenced by the anesthetic.", "PMID": 973690}
{"id": "pubmed23n0032_9445", "title": "Neurobehavioral responses and drug concentrations in newborns after maternal epidural anesthesia with bupivacaine.", "content": "The neurobehavioral status of 20 newborn infants was evaluated after two to four hours of life following maternal epidural anesthesia with bupivacaine for labor and vaginal delivery. All infants were normal products of uncomplicated full-term gestations. The 20 infants, whose mothers had received continuous lumbar epidural anesthesia with bupivacaine, demonstrated no measurable difference from control infants and did not have the decrease in muscle tone and strength observed in infants whose mothers had received continuous lumbar epidural anesthesia with lidocaine or mepivacaine in a previous study.", "contents": "Neurobehavioral responses and drug concentrations in newborns after maternal epidural anesthesia with bupivacaine. The neurobehavioral status of 20 newborn infants was evaluated after two to four hours of life following maternal epidural anesthesia with bupivacaine for labor and vaginal delivery. All infants were normal products of uncomplicated full-term gestations. The 20 infants, whose mothers had received continuous lumbar epidural anesthesia with bupivacaine, demonstrated no measurable difference from control infants and did not have the decrease in muscle tone and strength observed in infants whose mothers had received continuous lumbar epidural anesthesia with lidocaine or mepivacaine in a previous study.", "PMID": 973691}
{"id": "pubmed23n0032_9446", "title": "Amnesic actions of diazepam and scopolamine in man.", "content": "In man, diazepam alone and in combination with scopolamine interferes with the memory of visual and painful stimuli. With a 15-minute interval between injection of the drug and the showing of emotionally neutral pictures, scopolamine (0.5 mg/70 kg) produces 14 per cent forgetting when evaluated 24 hours later. Under these conditions diazepam (10 mg/70 kg) produces 41 per cent forgetting, while the combination causes 64 per cent. Under conditions designed to insure selection of subjects in whom registration was clearly quite intact at the time of the initial exposure to the pictures, memory was still found to be impaired when tested 24 hours later. Graded doses of diazepam to as much as mg/70 kg in combination with 0.5 mg/70 kg scopolamine produced a virtually linear dose-response curve for amnesia. These results are compatible with the interpretation that the diazepam-scopolamine mixture interferes with memory by blocking consolidation of the memory trace.", "contents": "Amnesic actions of diazepam and scopolamine in man. In man, diazepam alone and in combination with scopolamine interferes with the memory of visual and painful stimuli. With a 15-minute interval between injection of the drug and the showing of emotionally neutral pictures, scopolamine (0.5 mg/70 kg) produces 14 per cent forgetting when evaluated 24 hours later. Under these conditions diazepam (10 mg/70 kg) produces 41 per cent forgetting, while the combination causes 64 per cent. Under conditions designed to insure selection of subjects in whom registration was clearly quite intact at the time of the initial exposure to the pictures, memory was still found to be impaired when tested 24 hours later. Graded doses of diazepam to as much as mg/70 kg in combination with 0.5 mg/70 kg scopolamine produced a virtually linear dose-response curve for amnesia. These results are compatible with the interpretation that the diazepam-scopolamine mixture interferes with memory by blocking consolidation of the memory trace.", "PMID": 973692}
{"id": "pubmed23n0032_9447", "title": "Effects of halothane on DNA synthesis and the presynthetic phase (G 1) in dividing fibroblasts.", "content": "The effects of halothane on deoxyribonucleic acid (DNA) synthesis and  events preceding DNA synthesis have been examined in Chinese hamster fibroblasts in culture.DNA synthesis was studied by the uptake of 3H-thymidine during short periods of incubation that minimized effects on cells in the presynthetic phase (G1). Halothane produced slight but significant dose-related depression of 3H-thymidine uptake (20 per cent depression with 2 per cent halothane). In a separate series of experiments, synchronized cultures were exposed to 1-3 per cent halothane in G 1 phase for three or five hours. Halothane caused a postponement of onset of DNA synthesis (S phase), indicating a delay in G 1. This delay roughly equalled the duration of exposure to 3 per cent halothane but was less with 2 per cent halothane. The delay was only about one hour with 1 per cent halothane.", "contents": "Effects of halothane on DNA synthesis and the presynthetic phase (G 1) in dividing fibroblasts. The effects of halothane on deoxyribonucleic acid (DNA) synthesis and  events preceding DNA synthesis have been examined in Chinese hamster fibroblasts in culture.DNA synthesis was studied by the uptake of 3H-thymidine during short periods of incubation that minimized effects on cells in the presynthetic phase (G1). Halothane produced slight but significant dose-related depression of 3H-thymidine uptake (20 per cent depression with 2 per cent halothane). In a separate series of experiments, synchronized cultures were exposed to 1-3 per cent halothane in G 1 phase for three or five hours. Halothane caused a postponement of onset of DNA synthesis (S phase), indicating a delay in G 1. This delay roughly equalled the duration of exposure to 3 per cent halothane but was less with 2 per cent halothane. The delay was only about one hour with 1 per cent halothane.", "PMID": 973693}
{"id": "pubmed23n0032_9448", "title": "Closing capacity measurement during general anesthesia.", "content": "A modification of the single-breath nitrogen closing volume (CV) test allows measurement of closing capacity (CC) during general anesthesia. In the modification, inspiration and expiration are mechanically produced by a hydraulically powered cylinder. For 14 awake, normal subjects results of the CV test performed using this mechanical method differed that those obtained following spontaneous inspiration and expiration. Mean (+/-SE) CC's were 2.25 (+/-0.15) and 2.42 1 (+/-0.14) (P less than 0.01) using spontaneous and mechanical methods, respectively. The slopes of Phase III of the CV traces were 2.24 (+/-0.27) and 2.66 per cent N2/L (+/-0.32) (P less than 0.01), respectively. To eliminate differences due to measurement technique, the modified CV test was used both before and during anesthesia with halothane in 70 per cent N2 in 11 normal, supine, spontaneously breathing subjects. CC's were 1.89 l (+/-0.16) before and 1.84 l (+/-0.15) during anesthesia (P greater than .5). Mean functional residual capacities (FRC) by the closed-circuit helium method were 1.77 l (+/-0.15) before and 1.45 l (+/-0.17) during anesthesia (P less than .001). With CC unchanged and FRC decreased following induction, CC/FRC increased from 1.07 (+/-0.08) to 1.37 (+/-0.11) (P less than .005), suggesting increased small-airway closure during anesthesia.", "contents": "Closing capacity measurement during general anesthesia. A modification of the single-breath nitrogen closing volume (CV) test allows measurement of closing capacity (CC) during general anesthesia. In the modification, inspiration and expiration are mechanically produced by a hydraulically powered cylinder. For 14 awake, normal subjects results of the CV test performed using this mechanical method differed that those obtained following spontaneous inspiration and expiration. Mean (+/-SE) CC's were 2.25 (+/-0.15) and 2.42 1 (+/-0.14) (P less than 0.01) using spontaneous and mechanical methods, respectively. The slopes of Phase III of the CV traces were 2.24 (+/-0.27) and 2.66 per cent N2/L (+/-0.32) (P less than 0.01), respectively. To eliminate differences due to measurement technique, the modified CV test was used both before and during anesthesia with halothane in 70 per cent N2 in 11 normal, supine, spontaneously breathing subjects. CC's were 1.89 l (+/-0.16) before and 1.84 l (+/-0.15) during anesthesia (P greater than .5). Mean functional residual capacities (FRC) by the closed-circuit helium method were 1.77 l (+/-0.15) before and 1.45 l (+/-0.17) during anesthesia (P less than .001). With CC unchanged and FRC decreased following induction, CC/FRC increased from 1.07 (+/-0.08) to 1.37 (+/-0.11) (P less than .005), suggesting increased small-airway closure during anesthesia.", "PMID": 973703}
{"id": "pubmed23n0032_9449", "title": "Fluoride in bone of rats anesthetized during gestation with enflurane or methoxyflurane.", "content": "Fluoride concentrations in maternal and fetal bones were measured following exposure of pregnant rats to methoxyflurane and enflurane anesthesia. Fluoride content in fetal bone increased significantly only after exposure to methoxyflurane, and then only when methoxyflurane was administered after 12 days of gestation, when ossification of fetal bone begins. Fluoride concentrations in maternal bone increased following both anesthetics, except in rats exposed to methoxyflurane after 15 days of gestation, when ossification of many fetal skeletal parts is in progress and fluoride is preferentially deposited in the fetal skeleton.", "contents": "Fluoride in bone of rats anesthetized during gestation with enflurane or methoxyflurane. Fluoride concentrations in maternal and fetal bones were measured following exposure of pregnant rats to methoxyflurane and enflurane anesthesia. Fluoride content in fetal bone increased significantly only after exposure to methoxyflurane, and then only when methoxyflurane was administered after 12 days of gestation, when ossification of fetal bone begins. Fluoride concentrations in maternal bone increased following both anesthetics, except in rats exposed to methoxyflurane after 15 days of gestation, when ossification of many fetal skeletal parts is in progress and fluoride is preferentially deposited in the fetal skeleton.", "PMID": 973704}
{"id": "pubmed23n0032_9450", "title": "Distribution of waste anesthetic gases in the operating room air.", "content": "Epidemiologic and animal studies identify a strong relationship between chronic exposure to anesthetic gases and health hazards. Efforts to reduce exposure of personnel require an understanding of the distribution of anesthetic waste gases in the operating room air. Concentrations of nitrous oxide and halothane were measured at numerous stations throughout an operating room and a delivery room in the absence of personnel. Air conditioning flow rates and flow patterns were varied, as was the height of the anesthetic gas source. Air flow patterns were found to dominate the anesthetic gas distribution, while buoyancy effects were negligible. Venting waste gases at the floor does not significantly reduce exposure of personnel. Areas of high concentration were observed; their occurrences and locations varied strongly with air flow patterns. The exhaust grille is the best location for a single measurement of the average room concentration. Recirculating air-conditioning systems reduce energy costs; however, only the non-recirculating portion of the air exchanges reduces waste gas concentrations.", "contents": "Distribution of waste anesthetic gases in the operating room air. Epidemiologic and animal studies identify a strong relationship between chronic exposure to anesthetic gases and health hazards. Efforts to reduce exposure of personnel require an understanding of the distribution of anesthetic waste gases in the operating room air. Concentrations of nitrous oxide and halothane were measured at numerous stations throughout an operating room and a delivery room in the absence of personnel. Air conditioning flow rates and flow patterns were varied, as was the height of the anesthetic gas source. Air flow patterns were found to dominate the anesthetic gas distribution, while buoyancy effects were negligible. Venting waste gases at the floor does not significantly reduce exposure of personnel. Areas of high concentration were observed; their occurrences and locations varied strongly with air flow patterns. The exhaust grille is the best location for a single measurement of the average room concentration. Recirculating air-conditioning systems reduce energy costs; however, only the non-recirculating portion of the air exchanges reduces waste gas concentrations.", "PMID": 973705}
{"id": "pubmed23n0032_9451", "title": "Enflurane depresses myocardial function, perfusion, and metabolism in the dog.", "content": "Trained dogs with chronically implanted catheters and left ventricular (LV) pressure transducers were anesthetized with 2.3 per cent (1 + MAC) and 3.6 per cent enflurane. Left ventricular function and metabolism were studied while the dogs were awake and during exposure to the two anesthetic concentrations. Enflurane depressed LV function in a dose-dependent fashion. Myocardial blood flow and oxygenation mirrored the functional changes. Myocardial oxygen extraction decreased and lactate extraction increased to the myocardium. Low concentrations of halothane in the same dogs on different days had similar effects. However, 2 MAC halothane resembled 1.6 MAC enflurane, suggesting that the cardiovascular dose-effect curve for enflurane is steeper than that for halothane. Both anesthetics produce dose-dependent negative inotropic effects in the intact dog, accompanied by equivalent decreases in cardiac oxygen demand. Contrary to previous suggestions, enflurane appears to be at least at depressant to the dog heart as halothane.", "contents": "Enflurane depresses myocardial function, perfusion, and metabolism in the dog. Trained dogs with chronically implanted catheters and left ventricular (LV) pressure transducers were anesthetized with 2.3 per cent (1 + MAC) and 3.6 per cent enflurane. Left ventricular function and metabolism were studied while the dogs were awake and during exposure to the two anesthetic concentrations. Enflurane depressed LV function in a dose-dependent fashion. Myocardial blood flow and oxygenation mirrored the functional changes. Myocardial oxygen extraction decreased and lactate extraction increased to the myocardium. Low concentrations of halothane in the same dogs on different days had similar effects. However, 2 MAC halothane resembled 1.6 MAC enflurane, suggesting that the cardiovascular dose-effect curve for enflurane is steeper than that for halothane. Both anesthetics produce dose-dependent negative inotropic effects in the intact dog, accompanied by equivalent decreases in cardiac oxygen demand. Contrary to previous suggestions, enflurane appears to be at least at depressant to the dog heart as halothane.", "PMID": 973706}
{"id": "pubmed23n0032_9452", "title": "Pulmonary shunting during anesthesia with deliberate hypotension.", "content": "Pulmonary shunting (Qs/Qt with FIO2 = 1) was measured in 18 anesthetized patients during deliberate hypotension. Hypotension was induced in 12 patients with sodium nitroprusside and light halothane anesthesia and in six others with deep halothane anesthesia and mechanical hyperventilation. Similar results were observed in the two groups. During the hypotensive period mean arterial pressure (MAP) was reduced to 49 +/- 2 torr, a 37 per cent decrease from the control level after the onset of operation and a 40 per cent decrease compared with the recovery level during closure of the wound. Qs/Qt, however, remained unchanged throughout the study: 5.2 +/- 0.9 per cent initially, 5.4 +/- 0.8 per cent during hypotension, and 4.7 +/- 0.5 per cent during recovery. It is concluded that pulmonary shunting need not develop during deliberate hypotension induced with either technique.", "contents": "Pulmonary shunting during anesthesia with deliberate hypotension. Pulmonary shunting (Qs/Qt with FIO2 = 1) was measured in 18 anesthetized patients during deliberate hypotension. Hypotension was induced in 12 patients with sodium nitroprusside and light halothane anesthesia and in six others with deep halothane anesthesia and mechanical hyperventilation. Similar results were observed in the two groups. During the hypotensive period mean arterial pressure (MAP) was reduced to 49 +/- 2 torr, a 37 per cent decrease from the control level after the onset of operation and a 40 per cent decrease compared with the recovery level during closure of the wound. Qs/Qt, however, remained unchanged throughout the study: 5.2 +/- 0.9 per cent initially, 5.4 +/- 0.8 per cent during hypotension, and 4.7 +/- 0.5 per cent during recovery. It is concluded that pulmonary shunting need not develop during deliberate hypotension induced with either technique.", "PMID": 973707}
{"id": "pubmed23n0032_9453", "title": "Acid-base balance nomogram--a Boston-Copenhagen detente.", "content": "Quantitation of actual and expected complete compensation for chronic hypercapnia in man is facilitated by addition of data of Brackett et al. in the form of an arc to the Siggaard-Andersen alignment nomogram. Percentage of compensation is defined as 100 times the ratio of observed to predicted (fully compensated) base excess at the patient's PCO2. Extracellular fluid base excess, defined as BE3, is estimated from a 3-g hemoglobin line added to the nomogram.", "contents": "Acid-base balance nomogram--a Boston-Copenhagen detente. Quantitation of actual and expected complete compensation for chronic hypercapnia in man is facilitated by addition of data of Brackett et al. in the form of an arc to the Siggaard-Andersen alignment nomogram. Percentage of compensation is defined as 100 times the ratio of observed to predicted (fully compensated) base excess at the patient's PCO2. Extracellular fluid base excess, defined as BE3, is estimated from a 3-g hemoglobin line added to the nomogram.", "PMID": 973708}
{"id": "pubmed23n0032_9454", "title": "Primary aldosteronism with uncommon complications.", "content": "In a patient who had primary aldosteronism and severe total-body potassium depletion muscular tonic contractures developed during induction of anesthesia. After correction of the potassium deficit, the patient underwent uneventful anesthesia and transabdominal right adrenalectomy. Neither serum potassium level nor EKG seems to provide a reliable index of correction of potassium deficit. Measurement of potassium balance provided a method of quantitating the potassium depletion and of determining when the potassium deficit had been corrected. Balance studies should be utilized preoperatively when long-term potassium loss is suspected to reduced complication secondary to hypokalemia.", "contents": "Primary aldosteronism with uncommon complications. In a patient who had primary aldosteronism and severe total-body potassium depletion muscular tonic contractures developed during induction of anesthesia. After correction of the potassium deficit, the patient underwent uneventful anesthesia and transabdominal right adrenalectomy. Neither serum potassium level nor EKG seems to provide a reliable index of correction of potassium deficit. Measurement of potassium balance provided a method of quantitating the potassium depletion and of determining when the potassium deficit had been corrected. Balance studies should be utilized preoperatively when long-term potassium loss is suspected to reduced complication secondary to hypokalemia.", "PMID": 973709}
{"id": "pubmed23n0032_9455", "title": "Does measles really predispose to tuberculosis?", "content": "In recent years, textbooks and reviews on mechanisms involved in cellular immunity have emphasized that measles adversely influences tuberculous lesions. The medical literature was critically investigated for the basis of this claim. All field studies reviewed possessed strong deficiencies in the pertinent data and the methods of their collection. These deficiencies rendered invalid conclusions concerning the ability of measles to activate tuberculosis. Indirectly, there are also no adequate epidemiologic data to support the concept that measles suppresses tuberculous immunity when it suppresses tuberculin skin reactivity. Considering all the available information, one may conclude that if measles does have a deleterious influence on tuberculosis, it is probably at low frequency in most populations.", "contents": "Does measles really predispose to tuberculosis? In recent years, textbooks and reviews on mechanisms involved in cellular immunity have emphasized that measles adversely influences tuberculous lesions. The medical literature was critically investigated for the basis of this claim. All field studies reviewed possessed strong deficiencies in the pertinent data and the methods of their collection. These deficiencies rendered invalid conclusions concerning the ability of measles to activate tuberculosis. Indirectly, there are also no adequate epidemiologic data to support the concept that measles suppresses tuberculous immunity when it suppresses tuberculin skin reactivity. Considering all the available information, one may conclude that if measles does have a deleterious influence on tuberculosis, it is probably at low frequency in most populations.", "PMID": 973719}
{"id": "pubmed23n0032_9456", "title": "Use of fibrinogen/fibrin degradation products and soluble fibrin complexes for differentiating pulmonary embolism from nonthromboembolic lung disease.", "content": "To help differentiate pulmonary embolism from other lung diseases, we measured the degradation products of fibrinogen and fibrin and soluble fibrin complexes in normal control subjects and patients with pulmonary embolism, lung cancer, pneumonia, chronic obstructive pulmonary disease, tuberculosis, asthma, and several miscellaneous disorders. A separate group of patients, who were suspected of having pulmonary embolism but had negative pulmonary angiography, were also tested. Many nonthromboembolic lung diseases frequently were associated with positive fibrinogen/fibrin degradation products or soluble fibrin complexes, but those with high positivity rates for one test tended to have low rates for the other test. Both fibrinogen/fibrin degradation products and soluble fibrin complexes were positive in 55 per cent of patients with pulmonary embolism but only in 4 per cent with nonthromboembolic conditions (P less than 0.001), in 7 per cent of patients with negative pulmonary angiography (P less than 0.001), and in none of the normal subjects (P less than 0.001). Both tests were negative in only 3 per cent of patients with pulmonary embolism but in 35 per cent of nonthromboembolic diseases (P less than 0.005), 54 per cent of those with negative pulmonary angiography (P less than 0.001), and 79 per cent of normal control subjects (P less than 0.001). The combination of fibrinogen/fibrin degradation products and soluble fibrin complexes is more valuable than either test alone in the diagnostic separation of thromboembolic from nonthromboembolic pulmonary diseases.", "contents": "Use of fibrinogen/fibrin degradation products and soluble fibrin complexes for differentiating pulmonary embolism from nonthromboembolic lung disease. To help differentiate pulmonary embolism from other lung diseases, we measured the degradation products of fibrinogen and fibrin and soluble fibrin complexes in normal control subjects and patients with pulmonary embolism, lung cancer, pneumonia, chronic obstructive pulmonary disease, tuberculosis, asthma, and several miscellaneous disorders. A separate group of patients, who were suspected of having pulmonary embolism but had negative pulmonary angiography, were also tested. Many nonthromboembolic lung diseases frequently were associated with positive fibrinogen/fibrin degradation products or soluble fibrin complexes, but those with high positivity rates for one test tended to have low rates for the other test. Both fibrinogen/fibrin degradation products and soluble fibrin complexes were positive in 55 per cent of patients with pulmonary embolism but only in 4 per cent with nonthromboembolic conditions (P less than 0.001), in 7 per cent of patients with negative pulmonary angiography (P less than 0.001), and in none of the normal subjects (P less than 0.001). Both tests were negative in only 3 per cent of patients with pulmonary embolism but in 35 per cent of nonthromboembolic diseases (P less than 0.005), 54 per cent of those with negative pulmonary angiography (P less than 0.001), and 79 per cent of normal control subjects (P less than 0.001). The combination of fibrinogen/fibrin degradation products and soluble fibrin complexes is more valuable than either test alone in the diagnostic separation of thromboembolic from nonthromboembolic pulmonary diseases.", "PMID": 973720}
{"id": "pubmed23n0032_9457", "title": "Effects of intravenous histamine on static lung compliance and airway resistance in normal man.", "content": "Static lung compliance and airway resistance were measured in 30 experiments on 6 healthy subjects after intravenous injection of a bolus of histamine (4 mug per kg of body weight). Analysis of the pooled results showed a significant decrease in static lung compliance (maximal mean decrease,--16 per cent occurring 20 sec after injection). There was no significant change in functional residual capacity or vital capacity after injection of the histamine. Administration of propranolol (a beta-adrenergic blocking agent) did not significantly enhance the responses to histamine. The results suggested that humans, like other species, can constrict peripheral airways sufficiently to produce alterations in the static elastic properties of the lungs.", "contents": "Effects of intravenous histamine on static lung compliance and airway resistance in normal man. Static lung compliance and airway resistance were measured in 30 experiments on 6 healthy subjects after intravenous injection of a bolus of histamine (4 mug per kg of body weight). Analysis of the pooled results showed a significant decrease in static lung compliance (maximal mean decrease,--16 per cent occurring 20 sec after injection). There was no significant change in functional residual capacity or vital capacity after injection of the histamine. Administration of propranolol (a beta-adrenergic blocking agent) did not significantly enhance the responses to histamine. The results suggested that humans, like other species, can constrict peripheral airways sufficiently to produce alterations in the static elastic properties of the lungs.", "PMID": 973721}
{"id": "pubmed23n0032_9458", "title": "The relationship between symptoms and functional abnormalities in clinically healthy cigarette smokers.", "content": "Because a number of \"early tests\" may now be used to measure abnormalities of lung function in cigarette smokers with normal airflow rates, we evaluated the symptoms of cough, sputum, wheezing, and shortness of breath in 60 nonsmokers and 49 smokers to determine whether these symptoms correlated with measurements of lung function that are believed to be altered by mild obstruction. One or more symptoms were present in 5 per cent of the nonsmokers and 90 per cent of the smokers. Individual symptoms, sums of symptoms (symptoms score), and various combinations of symptoms correlated poorly or not at all to measured values for dynamic lung compliance, closing volume, closing capacity, slope of Phase III, and helium flow-volume curves. The symptoms score correlated negatively (P less than 0.05) with forced maximal expiratory flow at 50 per cent of vital capacity. Inasmuch as the serious consequences of chronic airway obstruction are related to abnormalities of lung function, it is likely the function tests will prove more useful than symptoms to reveal smokers at risk to develop chronic obstructive pulmonary disease. Symptoms cannot be used to detect smokers who are liable to have functional abnormalities suggestive of mild peripheral airway obstruction.", "contents": "The relationship between symptoms and functional abnormalities in clinically healthy cigarette smokers. Because a number of \"early tests\" may now be used to measure abnormalities of lung function in cigarette smokers with normal airflow rates, we evaluated the symptoms of cough, sputum, wheezing, and shortness of breath in 60 nonsmokers and 49 smokers to determine whether these symptoms correlated with measurements of lung function that are believed to be altered by mild obstruction. One or more symptoms were present in 5 per cent of the nonsmokers and 90 per cent of the smokers. Individual symptoms, sums of symptoms (symptoms score), and various combinations of symptoms correlated poorly or not at all to measured values for dynamic lung compliance, closing volume, closing capacity, slope of Phase III, and helium flow-volume curves. The symptoms score correlated negatively (P less than 0.05) with forced maximal expiratory flow at 50 per cent of vital capacity. Inasmuch as the serious consequences of chronic airway obstruction are related to abnormalities of lung function, it is likely the function tests will prove more useful than symptoms to reveal smokers at risk to develop chronic obstructive pulmonary disease. Symptoms cannot be used to detect smokers who are liable to have functional abnormalities suggestive of mild peripheral airway obstruction.", "PMID": 973722}
{"id": "pubmed23n0032_9459", "title": "Effect of meperidine on occlusion pressure responses to hypercapnia and hypoxia with and without external inspiratory resistance.", "content": "In 5 normal subjects we measured ventilation and P0.1, the pressure generated by the first 0.1 sec of inspiratory effort against a closed airway, in response to hypercapnia and hypoxia with and without added inspiratory resistance before and after oral meperidine (1.1 to 1.3 mg per kg). CO2 responses were studied in the steady state, whereas progressive hypoxia was used to elicit hypoxic responses. In general, resistance decreased ventilatory responses to hypercapnia but increased P0.1 responses to both hypoxia and hypercapnia. Meperidine depressed both ventilatory and P0.1 responses, more so in hypoxia than in hypercapnia. The combination of resistance and merperidine was additive in depressing responses to hypercapnia but in hypoxia produced little more depression than did meperidine alone. In both hypercapnia and hypoxia, meperidine decreased the augmentation of P0.1 that was associated with increased resistance. Normal subjects responded to acute increases of inspiratory resistance by increasing inspiratory motor output; this increase was distinctly blunted by meperidine.", "contents": "Effect of meperidine on occlusion pressure responses to hypercapnia and hypoxia with and without external inspiratory resistance. In 5 normal subjects we measured ventilation and P0.1, the pressure generated by the first 0.1 sec of inspiratory effort against a closed airway, in response to hypercapnia and hypoxia with and without added inspiratory resistance before and after oral meperidine (1.1 to 1.3 mg per kg). CO2 responses were studied in the steady state, whereas progressive hypoxia was used to elicit hypoxic responses. In general, resistance decreased ventilatory responses to hypercapnia but increased P0.1 responses to both hypoxia and hypercapnia. Meperidine depressed both ventilatory and P0.1 responses, more so in hypoxia than in hypercapnia. The combination of resistance and merperidine was additive in depressing responses to hypercapnia but in hypoxia produced little more depression than did meperidine alone. In both hypercapnia and hypoxia, meperidine decreased the augmentation of P0.1 that was associated with increased resistance. Normal subjects responded to acute increases of inspiratory resistance by increasing inspiratory motor output; this increase was distinctly blunted by meperidine.", "PMID": 973724}
{"id": "pubmed23n0032_9460", "title": "Effect of elastic loading on mouth occlusion pressure during CO2 rebreathing in man.", "content": "In 7 normal subjects, mouth occlusion pressure was evaluated as an index of neural drive to the respiratory muscles during CO2 rebreathing, with and without the addition of 2 degrees of elastic loads. During control and loaded rebreathing, changes in both mouth occlusion pressure and ventilation were linearly related to changes in end-tidal PCO2. With elastic loading, the slope of occlusion pressure versus end-tidal PCO2 response consistently increased from control values in all subjects and was greater with the higher load in 6 of 7 subjects. The ventilatory response to elastic loading was variable and inconsistent owing to the variable increase in frequency of breathing, the tidal volume always being diminished. In normal subjects, both mouth occlusion pressure and ventilation appeared to assess neural drive to the respiratory muscles in response to CO2 rebreathing; with elastic loading, only occlusion pressure continued to reflect neuromuscular output. This increased pressure response could have been mediated by neural reflex and/or intrinsic muscle mechanisms. The data suggest that mouth occlusion pressure may be a useful parameter for evaluating neuromuscular control mechanisms under conditions of increased lung elastance.", "contents": "Effect of elastic loading on mouth occlusion pressure during CO2 rebreathing in man. In 7 normal subjects, mouth occlusion pressure was evaluated as an index of neural drive to the respiratory muscles during CO2 rebreathing, with and without the addition of 2 degrees of elastic loads. During control and loaded rebreathing, changes in both mouth occlusion pressure and ventilation were linearly related to changes in end-tidal PCO2. With elastic loading, the slope of occlusion pressure versus end-tidal PCO2 response consistently increased from control values in all subjects and was greater with the higher load in 6 of 7 subjects. The ventilatory response to elastic loading was variable and inconsistent owing to the variable increase in frequency of breathing, the tidal volume always being diminished. In normal subjects, both mouth occlusion pressure and ventilation appeared to assess neural drive to the respiratory muscles in response to CO2 rebreathing; with elastic loading, only occlusion pressure continued to reflect neuromuscular output. This increased pressure response could have been mediated by neural reflex and/or intrinsic muscle mechanisms. The data suggest that mouth occlusion pressure may be a useful parameter for evaluating neuromuscular control mechanisms under conditions of increased lung elastance.", "PMID": 973725}
{"id": "pubmed23n0032_9461", "title": "Lung surface area in various morphologic forms of human emphysema.", "content": "The relation between lung surface area and the extent and morphologic type of emphysema was examined at autopsy in 69 men. It was found to be conceptually preferable to express the area as internal surface area per unit lung volume (specific surface area) rather than to use the more conventional, inversely related, mean linear intercept for comparison with other morphometric data. In the whole group, fixed inflated lung volume generally increased with increasing per cent involvement of lung parenchyma by emphysema, and total internal surface area decreased, but those changes were not so regular as the decline in specific surface area with increasing per cent emphysema (r=-0.574). Lungs with no emphysema had a significantly higher specific surface area than did lungs with very little emphysema (1 to 9 per cent), suggesting noticeable effects on the physical properties of lung by minimal degrees of this disease at high levels of inflation. The way in which specific surface area decreased with increasing percentage of emphysema was not noticeably different among patients with centrilobular, panlobular, or mixed forms of emphysema, although lungs with only centrilobular emphysema were much more common and had a significantly lower mean percentage of emphysema than did those with only panlobular emphysema; the prevalence and mean percentage of emphysema of the mixed group were intermediate. These observations, coupled with the association of older mean age with increasing percentage of emphysema, were interpreted as support for the concept that many examples of panlobular emphysema are derived from confluence of extensive centrilobular emphysematous lesions.", "contents": "Lung surface area in various morphologic forms of human emphysema. The relation between lung surface area and the extent and morphologic type of emphysema was examined at autopsy in 69 men. It was found to be conceptually preferable to express the area as internal surface area per unit lung volume (specific surface area) rather than to use the more conventional, inversely related, mean linear intercept for comparison with other morphometric data. In the whole group, fixed inflated lung volume generally increased with increasing per cent involvement of lung parenchyma by emphysema, and total internal surface area decreased, but those changes were not so regular as the decline in specific surface area with increasing per cent emphysema (r=-0.574). Lungs with no emphysema had a significantly higher specific surface area than did lungs with very little emphysema (1 to 9 per cent), suggesting noticeable effects on the physical properties of lung by minimal degrees of this disease at high levels of inflation. The way in which specific surface area decreased with increasing percentage of emphysema was not noticeably different among patients with centrilobular, panlobular, or mixed forms of emphysema, although lungs with only centrilobular emphysema were much more common and had a significantly lower mean percentage of emphysema than did those with only panlobular emphysema; the prevalence and mean percentage of emphysema of the mixed group were intermediate. These observations, coupled with the association of older mean age with increasing percentage of emphysema, were interpreted as support for the concept that many examples of panlobular emphysema are derived from confluence of extensive centrilobular emphysematous lesions.", "PMID": 973726}
{"id": "pubmed23n0032_9462", "title": "Pulmonary sporotrichosis: Treatment with miconazole.", "content": "Intravenous miconazole, an investigational, broad-spectrum, antifungal imidazole drug, was clinically effective when used for the first time in a patient with life-threatening sporotrichosis. Miconazole was used after drug resistance and treatment failure occurred in a patient with extensive, cavitary, pulmonary infection with Sporotrichum schenckii. He had first been treated with amphotericin B, and then with amphotericin B and flucytosine in combination. Side effects of miconazole included itching, diarrhea, and phlebitis. Clinical improvement occurred despite plasma concentrations below those needed for complete inhibition of the patient's organisms in vitro.", "contents": "Pulmonary sporotrichosis: Treatment with miconazole. Intravenous miconazole, an investigational, broad-spectrum, antifungal imidazole drug, was clinically effective when used for the first time in a patient with life-threatening sporotrichosis. Miconazole was used after drug resistance and treatment failure occurred in a patient with extensive, cavitary, pulmonary infection with Sporotrichum schenckii. He had first been treated with amphotericin B, and then with amphotericin B and flucytosine in combination. Side effects of miconazole included itching, diarrhea, and phlebitis. Clinical improvement occurred despite plasma concentrations below those needed for complete inhibition of the patient's organisms in vitro.", "PMID": 973727}
{"id": "pubmed23n0032_9463", "title": "Antioxidants and cancer. Part VI. Selenium and age-adjusted human cancer mortality.", "content": "The possible epidemiologic relationship between selenium occurrence and cancer mortality was studied in cities and states located in areas with different levels of selenium bioavailability. Statistically significant differences were found in age-specific cancer death rates among states with high, medium, and low selenium levels. The death rates for specific types of cancer showed a larger difference in males than in females in the states with high selenium levels. The greater difference between males and females may be related to sex difference or to the fact that males are heavier smokers and are aslo more likely to be exposed to industrial pollution. In the states with high selenium levels, there was significantly lower mortality in both males and females from several types of cancer, particularly the environmental problem indicators, such as gastrointestinal and urogenital types of cancer.", "contents": "Antioxidants and cancer. Part VI. Selenium and age-adjusted human cancer mortality. The possible epidemiologic relationship between selenium occurrence and cancer mortality was studied in cities and states located in areas with different levels of selenium bioavailability. Statistically significant differences were found in age-specific cancer death rates among states with high, medium, and low selenium levels. The death rates for specific types of cancer showed a larger difference in males than in females in the states with high selenium levels. The greater difference between males and females may be related to sex difference or to the fact that males are heavier smokers and are aslo more likely to be exposed to industrial pollution. In the states with high selenium levels, there was significantly lower mortality in both males and females from several types of cancer, particularly the environmental problem indicators, such as gastrointestinal and urogenital types of cancer.", "PMID": 973735}
{"id": "pubmed23n0032_9464", "title": "Respiratory effects of occupational exposure to an epoxy resin system.", "content": "A standardized respiratory questionnaire and pulmonary function tests were used to examine thirty-four employees of a snow-ski manufacturing plant, including twenty-five workers who were exposed to an epoxy resin system containing the amine hardener 3-dimethylamino propylamine (3-DMAPA). Maximum expiratory flow-volume curves were obtained on Monday and Thursday, before and after each shift, and FVC, FEV1.0, MEF50%, and MEF25% were caculated. Environmental measurements of the total amine levels were found to range from 0.41 to 1.38 ppm. The group with the greatest exposure (0.55-1.38 ppm) showed significant decreases in lung function over Monday and over the week. Although all employees in this group showed decreases in pulmonary function, acute changes were greater in present cigarette smokers and in subjects who reported respiratory symptoms upon exposure to the epoxy resin system. There was no evidence of permanent loss of lung function in subjects with either the highest or longest exposure.", "contents": "Respiratory effects of occupational exposure to an epoxy resin system. A standardized respiratory questionnaire and pulmonary function tests were used to examine thirty-four employees of a snow-ski manufacturing plant, including twenty-five workers who were exposed to an epoxy resin system containing the amine hardener 3-dimethylamino propylamine (3-DMAPA). Maximum expiratory flow-volume curves were obtained on Monday and Thursday, before and after each shift, and FVC, FEV1.0, MEF50%, and MEF25% were caculated. Environmental measurements of the total amine levels were found to range from 0.41 to 1.38 ppm. The group with the greatest exposure (0.55-1.38 ppm) showed significant decreases in lung function over Monday and over the week. Although all employees in this group showed decreases in pulmonary function, acute changes were greater in present cigarette smokers and in subjects who reported respiratory symptoms upon exposure to the epoxy resin system. There was no evidence of permanent loss of lung function in subjects with either the highest or longest exposure.", "PMID": 973736}
{"id": "pubmed23n0032_9465", "title": "Cell vs. noncell airway temporal response in rats exposed to sulfur dioxide.", "content": "A modification of the sulfur dioxide (SO2)-exposed rat model proposed by Reid for the study of chronic bronchitis was employed to evaluate mucus retention and cytologic changes. Rats were exposed to from 600 to 700 ppm of SO2 for 3 hours per day, and groups were examined after 0, 9, 18, and 30 hours of cumulative exposure. Tracheal mucus retention and statistically significant increases (two- to four-fold) in the amount of solid material (cellular and mucus) recovered by bronchia lavage developed as a function of exposure time. The increase in bronchial solids was primarily due to inflammatory cells. The goblet cell population in secondary bronchi declined initially, with a significant increase after 30 hours of exposure (P less than .001). The cytologic data provide additional support to Reid's suggestion that the SO2-exposed rat may be considered as a chronic bronchitis model.", "contents": "Cell vs. noncell airway temporal response in rats exposed to sulfur dioxide. A modification of the sulfur dioxide (SO2)-exposed rat model proposed by Reid for the study of chronic bronchitis was employed to evaluate mucus retention and cytologic changes. Rats were exposed to from 600 to 700 ppm of SO2 for 3 hours per day, and groups were examined after 0, 9, 18, and 30 hours of cumulative exposure. Tracheal mucus retention and statistically significant increases (two- to four-fold) in the amount of solid material (cellular and mucus) recovered by bronchia lavage developed as a function of exposure time. The increase in bronchial solids was primarily due to inflammatory cells. The goblet cell population in secondary bronchi declined initially, with a significant increase after 30 hours of exposure (P less than .001). The cytologic data provide additional support to Reid's suggestion that the SO2-exposed rat may be considered as a chronic bronchitis model.", "PMID": 973737}
{"id": "pubmed23n0032_9466", "title": "Adult mortality in two communities with widely different air pollution levels.", "content": "Mortality experience was determined over a 13-year period (1960-72) for sample populations in two small Pennsylvania communities with widely different air pollution levels. The sample populations had been the subject of a Public Health Service study at the beginning of the period and air quality measurements were made at that time. The influence of smoking on mortality was clearly evident. A relationship was suggested between mortality rate and length of residence in the polluted community (age-adjusted), but not in the control community. The hypothesis is suggested that immigrants to a polluted area are a self-selected, unusually healthy group. While the small population size precluded definitive conclusions on the influence of air pollution on mortality, it appears that those with over 20 years exposure to air pollution in the polluted community (151 mug/m3 suspended particulate, 3.7 mg/100 cm2/day sulfation rate) have about one-tenth the excess mortality of those smoking one pack of cigarettes a day in the control community (109 mug/m3, 0.6 mg/100 cm2/day). The data are consistent with the hypothesis that the effects of smoking and air pollution are additive.", "contents": "Adult mortality in two communities with widely different air pollution levels. Mortality experience was determined over a 13-year period (1960-72) for sample populations in two small Pennsylvania communities with widely different air pollution levels. The sample populations had been the subject of a Public Health Service study at the beginning of the period and air quality measurements were made at that time. The influence of smoking on mortality was clearly evident. A relationship was suggested between mortality rate and length of residence in the polluted community (age-adjusted), but not in the control community. The hypothesis is suggested that immigrants to a polluted area are a self-selected, unusually healthy group. While the small population size precluded definitive conclusions on the influence of air pollution on mortality, it appears that those with over 20 years exposure to air pollution in the polluted community (151 mug/m3 suspended particulate, 3.7 mg/100 cm2/day sulfation rate) have about one-tenth the excess mortality of those smoking one pack of cigarettes a day in the control community (109 mug/m3, 0.6 mg/100 cm2/day). The data are consistent with the hypothesis that the effects of smoking and air pollution are additive.", "PMID": 973738}
{"id": "pubmed23n0032_9467", "title": "Trace metal studies in Bushman hair.", "content": "Bushman women appear to have sufficient stores of iron and zinc during pregnancy and lactation; as a result, iron deficiency anemia is rare in Bushman women, even in pregnancy. Hair samples from Bushman women (young nonpregnant, lactating, and postmenopausal) and men were analyzed for iron, zinc, and copper content. Values obtained were compared with those for groups of Bantu and American inner-city mothers. Mean iron levels in the hair were found to be high (173 ppm) for lactating Bushman women, as compared with 43.2 ppm in the group of Bantu women, and 22.9 ppm in the group of American inner-city women. Zinc levels also were higher in the Bushman women. Mean copper values were much higher in the American inner-city mothers (19.9 ppm) than in the Bushman women (8.0 ppm) or in the lactating Bantu women (9.9 ppm). The sum of the metals may be more important than the individual concentrations.", "contents": "Trace metal studies in Bushman hair. Bushman women appear to have sufficient stores of iron and zinc during pregnancy and lactation; as a result, iron deficiency anemia is rare in Bushman women, even in pregnancy. Hair samples from Bushman women (young nonpregnant, lactating, and postmenopausal) and men were analyzed for iron, zinc, and copper content. Values obtained were compared with those for groups of Bantu and American inner-city mothers. Mean iron levels in the hair were found to be high (173 ppm) for lactating Bushman women, as compared with 43.2 ppm in the group of Bantu women, and 22.9 ppm in the group of American inner-city women. Zinc levels also were higher in the Bushman women. Mean copper values were much higher in the American inner-city mothers (19.9 ppm) than in the Bushman women (8.0 ppm) or in the lactating Bantu women (9.9 ppm). The sum of the metals may be more important than the individual concentrations.", "PMID": 973739}
{"id": "pubmed23n0032_9468", "title": "Low-tar and high-tar cigarettes.", "content": "Mice were exposed for 7 to 8 minutes on weekdays to fresh smoke from high-tar (HT) or low-tar (LT) cigarettes for varying periods of up to 36 weeks. Mice exposed to HT cigarettes exhibited more marked alterations in humoral immune responsiveness, hematological profiles, and pulmonary pathologic findings than those exposed to LT cigarettes. However, cell-mediated immune responsiveness to both bacterial and tumor-specific antigens was depressed similarly in animals exposed to HT or LT cigarettes. Furthermore, the growth rates of subcutaneously established tumors were enhanced similarly in the two groups, with respect to those in control animals.", "contents": "Low-tar and high-tar cigarettes. Mice were exposed for 7 to 8 minutes on weekdays to fresh smoke from high-tar (HT) or low-tar (LT) cigarettes for varying periods of up to 36 weeks. Mice exposed to HT cigarettes exhibited more marked alterations in humoral immune responsiveness, hematological profiles, and pulmonary pathologic findings than those exposed to LT cigarettes. However, cell-mediated immune responsiveness to both bacterial and tumor-specific antigens was depressed similarly in animals exposed to HT or LT cigarettes. Furthermore, the growth rates of subcutaneously established tumors were enhanced similarly in the two groups, with respect to those in control animals.", "PMID": 973740}
{"id": "pubmed23n0032_9469", "title": "[Plant antigens with immunological properties. II. Further studies of the chemical characteristics and immunological properties of a grass pollen extract].", "content": "This second study carried out on an extract of two grass pollens (Poa pratensis and Phleum pratense) demonstrated the presence of N-acetilglucosamine and sialic acid in the extract (RFEX). Moreover the extract has confirmed its characteristics biologic activity i.e. the inhibition of the phenomenon of immune hemolysis with a different technique too, even having the antibodies of the treated animals the complement fixing activity. No appreciable changes of the immunoelectrophoresis pattern in the serum of the immunized mice have been noticed.", "contents": "[Plant antigens with immunological properties. II. Further studies of the chemical characteristics and immunological properties of a grass pollen extract]. This second study carried out on an extract of two grass pollens (Poa pratensis and Phleum pratense) demonstrated the presence of N-acetilglucosamine and sialic acid in the extract (RFEX). Moreover the extract has confirmed its characteristics biologic activity i.e. the inhibition of the phenomenon of immune hemolysis with a different technique too, even having the antibodies of the treated animals the complement fixing activity. No appreciable changes of the immunoelectrophoresis pattern in the serum of the immunized mice have been noticed.", "PMID": 973743}
{"id": "pubmed23n0032_9470", "title": "Vagotomy and drainage procedure for duodenal ulcer: The results of seventeen years' experience.", "content": "During the 10 years prior to January, 1968, 455 duodenal ulcer patients were operated upon at the University of Florida affiliated hospitals. The early results were reported in 1969. The present study is a followup of the same patients now 7 to 17 years after vagotomy and drainage. Twenty-four per cent were lost to followup. The ulcer recurrence rate was 5.8 per cent. All the recurrent ulcers that were not gastric in location (4.9%) occurred within 5 years after the original operative procedure with a mean of 2.6 years. The gastric ulcers (0.9%) occurred at a much later date with a mean of 6 years. It is concluded that vagotomy and gastric drainage is a satisfactory modality of therapy for duodenal ulcer. It can be accomplished with an acceptable morbidity and mortality and the long-term recurrence rate is low. If recurrences due to incomplete vagotomy do not occur within 5 years, they are unlikely to recur.", "contents": "Vagotomy and drainage procedure for duodenal ulcer: The results of seventeen years' experience. During the 10 years prior to January, 1968, 455 duodenal ulcer patients were operated upon at the University of Florida affiliated hospitals. The early results were reported in 1969. The present study is a followup of the same patients now 7 to 17 years after vagotomy and drainage. Twenty-four per cent were lost to followup. The ulcer recurrence rate was 5.8 per cent. All the recurrent ulcers that were not gastric in location (4.9%) occurred within 5 years after the original operative procedure with a mean of 2.6 years. The gastric ulcers (0.9%) occurred at a much later date with a mean of 6 years. It is concluded that vagotomy and gastric drainage is a satisfactory modality of therapy for duodenal ulcer. It can be accomplished with an acceptable morbidity and mortality and the long-term recurrence rate is low. If recurrences due to incomplete vagotomy do not occur within 5 years, they are unlikely to recur.", "PMID": 973748}
{"id": "pubmed23n0032_9471", "title": "A porspective study of parietal cell vagotomy and selective vagotomy-antrectomy for treatment of duodenal ulcer.", "content": "A prospective, randomized, study involving 92 patients who required elective operation for treatment of duodenal ulcer was performed to compare the results of Parietal Cell Vagotomy (PCV) and selective vagotomy-antrectomy Billroth I (SV-A-BI). The protocol was broken twice. One patient was unable to undergo PCV because of pyloric stenosis and one patients underwent Billroth II anastomosis instead of Billroth I because of post-bulbar stenosis. Performance of PCV was never aborted because a patient was obese. There were no deaths. Diarrhea, dumping and other gastric complaints were less frequent after PCV than after SV-A-BI for all time periods studies up to two years. Two months after operation, the Hollander tests were negative in 59% of patients after PCV and in 100% after SV-ABI. Inhibition of Bao and MAO were also significantly less after PCV than after SV-A-BI. Since vagotomy of the parietal cell mass was identical in both groups of patients it was concluded that the differences in the secretory rates and the fewer negative Hollander tests in the PCV group than in the SV-A-BI group were due to retention of the antrum irrespective of its innervation. There was no explanation for the gradual increase in the BAO in the PCV group. One recurrent ulcer occurred in the PCV group in a patient who overindulged in alcohol and aspirin. After 4 days of medical management, this superficial ulcer healed as demonstrated by endoscopy. There were no recurrent ulcers after SV-A-BI. As a result of this study, it is concluded that PCV is superior to SV-A-BI because of the lower frequency of postoperative complications, diarrhea, dumping and other symptoms associated with gastric surgery. PCV may be the operation of choice for the elective treatment of duodenal ulcer; however, it remains undetermined whether the recurrent ulcer rate following PCV will be sufficiently low that the procedure can retain a position of superiority over SV-A-BI.", "contents": "A porspective study of parietal cell vagotomy and selective vagotomy-antrectomy for treatment of duodenal ulcer. A prospective, randomized, study involving 92 patients who required elective operation for treatment of duodenal ulcer was performed to compare the results of Parietal Cell Vagotomy (PCV) and selective vagotomy-antrectomy Billroth I (SV-A-BI). The protocol was broken twice. One patient was unable to undergo PCV because of pyloric stenosis and one patients underwent Billroth II anastomosis instead of Billroth I because of post-bulbar stenosis. Performance of PCV was never aborted because a patient was obese. There were no deaths. Diarrhea, dumping and other gastric complaints were less frequent after PCV than after SV-A-BI for all time periods studies up to two years. Two months after operation, the Hollander tests were negative in 59% of patients after PCV and in 100% after SV-ABI. Inhibition of Bao and MAO were also significantly less after PCV than after SV-A-BI. Since vagotomy of the parietal cell mass was identical in both groups of patients it was concluded that the differences in the secretory rates and the fewer negative Hollander tests in the PCV group than in the SV-A-BI group were due to retention of the antrum irrespective of its innervation. There was no explanation for the gradual increase in the BAO in the PCV group. One recurrent ulcer occurred in the PCV group in a patient who overindulged in alcohol and aspirin. After 4 days of medical management, this superficial ulcer healed as demonstrated by endoscopy. There were no recurrent ulcers after SV-A-BI. As a result of this study, it is concluded that PCV is superior to SV-A-BI because of the lower frequency of postoperative complications, diarrhea, dumping and other symptoms associated with gastric surgery. PCV may be the operation of choice for the elective treatment of duodenal ulcer; however, it remains undetermined whether the recurrent ulcer rate following PCV will be sufficiently low that the procedure can retain a position of superiority over SV-A-BI.", "PMID": 973749}
{"id": "pubmed23n0032_9472", "title": "[Studies of the enterotoxin production capacity of L forms derived from enterotoxic strains of Staphylococcus aureus].", "content": "The production of enterotoxin by L-forms obtained from strains of Staphylococcus aureus (10 enterotoxin A-producing strains, 7 enterotoxin B, 2 enterotoxin C and 1 which produced both enterotoxins A and C) has been studied by agar-gel diffusion technique. Using a proper technique, enterotoxin could be detected for 4 L-forms; 3 of these produced the same enterotoxin as parent strain (2 enterotoxin A and 1 enterotoxin B). As regards the other L-form, obtained from strain of St. Aureus which produced both enterotoxins A and C, only enterotoxin A could be detected.", "contents": "[Studies of the enterotoxin production capacity of L forms derived from enterotoxic strains of Staphylococcus aureus]. The production of enterotoxin by L-forms obtained from strains of Staphylococcus aureus (10 enterotoxin A-producing strains, 7 enterotoxin B, 2 enterotoxin C and 1 which produced both enterotoxins A and C) has been studied by agar-gel diffusion technique. Using a proper technique, enterotoxin could be detected for 4 L-forms; 3 of these produced the same enterotoxin as parent strain (2 enterotoxin A and 1 enterotoxin B). As regards the other L-form, obtained from strain of St. Aureus which produced both enterotoxins A and C, only enterotoxin A could be detected.", "PMID": 973745}
{"id": "pubmed23n0032_9473", "title": "Complications of vagotomy in the treatment of hiatal hernia.", "content": "Inclusion of vagotomy and pyloroplasty in the surgical treatment of gastroesophageal reflux associated with hiatal hernia has long been controversial. To evaluate the morbidity of vagotomy in the treatment of reflux esophagitis, a retrospective study of 311 patients treated by the Hill posterior gastropexy technique of hiatal hernia repair was tabulated. Vagotomy with the anti-reflux operation was performed upon 159 patients (51%). Vagotomy was not included for 152 patients (49%). The incidence of postoperative symptoms with or without vagotomy was almost equally divided--41% without vagotomy and 47% with vagotomy. However, the major postoperative symptoms that occurred in both groups were abdominal cramps and bloating which usually disappeared in the early postoperative period and were attributed to the anti-reflux procedure and not to vagotomy. When vagotomy was included with the anti-reflux operation, the incidence and duration of long term, disabling postoperative symptoms were significantly increased. Diarrhea occurred two times more frequently. Nausea and vomiting occurred ten times more frequently and dumping was present only in vagotomized patients. Long term postoperative symptoms, judged on a basis of symptoms lasting longer than three months duration, occurred in 1% of patients without vagotomy and 26% when vagotomy was included. This study revealed that no additional protection against recurrent symptoms of gastroesophageal reflux or radiographic evidence of recurrent hiatal hernia was provided by inclusion of vagotomy. In conclusion, vagotomy is contraindicated in the treatment of gastroesophageal reflux except in the presence of peptic ulcer disease.", "contents": "Complications of vagotomy in the treatment of hiatal hernia. Inclusion of vagotomy and pyloroplasty in the surgical treatment of gastroesophageal reflux associated with hiatal hernia has long been controversial. To evaluate the morbidity of vagotomy in the treatment of reflux esophagitis, a retrospective study of 311 patients treated by the Hill posterior gastropexy technique of hiatal hernia repair was tabulated. Vagotomy with the anti-reflux operation was performed upon 159 patients (51%). Vagotomy was not included for 152 patients (49%). The incidence of postoperative symptoms with or without vagotomy was almost equally divided--41% without vagotomy and 47% with vagotomy. However, the major postoperative symptoms that occurred in both groups were abdominal cramps and bloating which usually disappeared in the early postoperative period and were attributed to the anti-reflux procedure and not to vagotomy. When vagotomy was included with the anti-reflux operation, the incidence and duration of long term, disabling postoperative symptoms were significantly increased. Diarrhea occurred two times more frequently. Nausea and vomiting occurred ten times more frequently and dumping was present only in vagotomized patients. Long term postoperative symptoms, judged on a basis of symptoms lasting longer than three months duration, occurred in 1% of patients without vagotomy and 26% when vagotomy was included. This study revealed that no additional protection against recurrent symptoms of gastroesophageal reflux or radiographic evidence of recurrent hiatal hernia was provided by inclusion of vagotomy. In conclusion, vagotomy is contraindicated in the treatment of gastroesophageal reflux except in the presence of peptic ulcer disease.", "PMID": 973750}
{"id": "pubmed23n0032_9474", "title": "Acute demeton poisoning in a child.", "content": "An acute case of demeton poisoning in a child is described. Intially, typical signs of organophosphate poisoning were not present, and the illness was thought to be aliphatic hydrocarbon (fuel oil) intoxication. When correct diagnosis was first suspected, treatment with pralidoxime chloride and atropine sulfate produced some perilous complications, but was probably life-saving ultimately. Plasma (pseudo) cholinesterase levels 15 to 41 hours after exposure ranged from 0.24 to 0.48 international units (IU). Approximate normal value (2.5 IU) was not reached until 5 1/2 days after exposure. Transdermal poisoning by demeton was confirmed by its presence (15.4 mg/l) in the suspected fuel oil and, at high levels, in several articles of the child's clothing. Verification of expected urinary metabolites helped confirm the diagnosis of demeton poisoning. The availability of rapid blood cholinesterase field tests in hospital laboratories, especially in agricultural areas, is pointed out as an urgent need.", "contents": "Acute demeton poisoning in a child. An acute case of demeton poisoning in a child is described. Intially, typical signs of organophosphate poisoning were not present, and the illness was thought to be aliphatic hydrocarbon (fuel oil) intoxication. When correct diagnosis was first suspected, treatment with pralidoxime chloride and atropine sulfate produced some perilous complications, but was probably life-saving ultimately. Plasma (pseudo) cholinesterase levels 15 to 41 hours after exposure ranged from 0.24 to 0.48 international units (IU). Approximate normal value (2.5 IU) was not reached until 5 1/2 days after exposure. Transdermal poisoning by demeton was confirmed by its presence (15.4 mg/l) in the suspected fuel oil and, at high levels, in several articles of the child's clothing. Verification of expected urinary metabolites helped confirm the diagnosis of demeton poisoning. The availability of rapid blood cholinesterase field tests in hospital laboratories, especially in agricultural areas, is pointed out as an urgent need.", "PMID": 973741}
{"id": "pubmed23n0032_9475", "title": "Total duodenal diversion for treatment of reflux esophagitis uncontrolled by repeated antireflux procedures.", "content": "The operations of Nissen, Hill, and Belsey are adequate in controlling esophaegeal reflux in the majority of patients. In a small percentage however, objective and subjective evidence of esophagitis persists in spite of repeated operations to restore lower esophageal sphincter competency. These failures are then usually treated by operative procedures of great magnitude involving organ interposition. Repeated antireflux operations directed to the gastroesophageal area may in some instances result in impairment of blood supply with an increased risk of both esophageal and gastric fistulae. In the past many observers have felt that reflux esophagitis resulted solely from the effects of acid-pepsin secretions bathing the distal esophagus. Recently experimental and clinical data have indicated the importance of duodenal contents in the etiology and perpetuation of reflux esophagitis. During a recent two year period, 6 patients with persistent reflux esophagitis uncontrolled by repeated antireflux procedures have been seen on our service. These 6 patients, underwent 12 unsuccessful antireflux operations elsewhere. Three of the 6 patients had also been subjected to vagotomy-antrectomy for a coexisting duodenal ulcer. A marked lowering of gastric acidity took place but esophageal reflux and esophagitis persisted. These three patients were treated on our service by takedown of the Billroth I anastomosis, closure of the duodenal stump and diversion of the duodenal contents into a Roux-en-Y limb. Three other patients who had undergone unsuccessful antireflux procedures alone were subjected to antral resection, Roux-en-Y diversion and transthoracid vagotomy. This simplified appraoch to the treatment of persistent esophageal reflux uncontrolled by repeated antireflux procedures has given satisfactory results. The operation should be considered when technical considerations preclude further surgical attempts to perform another effective antireflux operation. Total duodenal diversion should, however, not be considered as the primary operation for the patient suffering from reflux esophagitis. However, in circumstances discussed above this direct approach appears preferable to major resectional procedures.", "contents": "Total duodenal diversion for treatment of reflux esophagitis uncontrolled by repeated antireflux procedures. The operations of Nissen, Hill, and Belsey are adequate in controlling esophaegeal reflux in the majority of patients. In a small percentage however, objective and subjective evidence of esophagitis persists in spite of repeated operations to restore lower esophageal sphincter competency. These failures are then usually treated by operative procedures of great magnitude involving organ interposition. Repeated antireflux operations directed to the gastroesophageal area may in some instances result in impairment of blood supply with an increased risk of both esophageal and gastric fistulae. In the past many observers have felt that reflux esophagitis resulted solely from the effects of acid-pepsin secretions bathing the distal esophagus. Recently experimental and clinical data have indicated the importance of duodenal contents in the etiology and perpetuation of reflux esophagitis. During a recent two year period, 6 patients with persistent reflux esophagitis uncontrolled by repeated antireflux procedures have been seen on our service. These 6 patients, underwent 12 unsuccessful antireflux operations elsewhere. Three of the 6 patients had also been subjected to vagotomy-antrectomy for a coexisting duodenal ulcer. A marked lowering of gastric acidity took place but esophageal reflux and esophagitis persisted. These three patients were treated on our service by takedown of the Billroth I anastomosis, closure of the duodenal stump and diversion of the duodenal contents into a Roux-en-Y limb. Three other patients who had undergone unsuccessful antireflux procedures alone were subjected to antral resection, Roux-en-Y diversion and transthoracid vagotomy. This simplified appraoch to the treatment of persistent esophageal reflux uncontrolled by repeated antireflux procedures has given satisfactory results. The operation should be considered when technical considerations preclude further surgical attempts to perform another effective antireflux operation. Total duodenal diversion should, however, not be considered as the primary operation for the patient suffering from reflux esophagitis. However, in circumstances discussed above this direct approach appears preferable to major resectional procedures.", "PMID": 973751}
{"id": "pubmed23n0032_9476", "title": "[Studies of some biochemical characteristics and antibiotic sensitivity of 30 L strains of Staphylococcus aureus].", "content": "The production of coagulase, DNase and haemolysins by 30 L-forms obtained from as many strains of Staphylococcus aureus having these properties, has been studied using different techniques. All of L-strains produced coagulase, for the most part after incubation for 24 h, but only some produced DNase and haemolisins. At the same time, the sensitivities of various antibiotics of staphyloccal L-forms and the parent strains has been determined. All of L-forms, as regards to bacterial form from which they were derived, showed an high resistance to antibiotics of which the primary site of action is upon cell wall (penicillin, ampicillin, methicillin, cephalosporins, vancomycin), with the exception of bacitracin. The tetracycline was more effective against the L-forms, while the group that includes chloramphenicol, novobiocin, streptomycin, gentamycin and kanamycin inhibited coccal and L-forms about equally.", "contents": "[Studies of some biochemical characteristics and antibiotic sensitivity of 30 L strains of Staphylococcus aureus]. The production of coagulase, DNase and haemolysins by 30 L-forms obtained from as many strains of Staphylococcus aureus having these properties, has been studied using different techniques. All of L-strains produced coagulase, for the most part after incubation for 24 h, but only some produced DNase and haemolisins. At the same time, the sensitivities of various antibiotics of staphyloccal L-forms and the parent strains has been determined. All of L-forms, as regards to bacterial form from which they were derived, showed an high resistance to antibiotics of which the primary site of action is upon cell wall (penicillin, ampicillin, methicillin, cephalosporins, vancomycin), with the exception of bacitracin. The tetracycline was more effective against the L-forms, while the group that includes chloramphenicol, novobiocin, streptomycin, gentamycin and kanamycin inhibited coccal and L-forms about equally.", "PMID": 973744}
{"id": "pubmed23n0032_9477", "title": "A current appraisal of problems with gangrenous bowel.", "content": "Gangrenous bowel most often results from hernia, adhesions and mesenteric insufficiency. The overall mortality rate for 151 cases was 37%. This figure was 20% for hernia, 23% for adhesions and 74% for mesenteric insufficiency. In the latter category where bowel resection was feasable the mortality rate was 40%. Other causes of bowel gangrene had a mortality rate of 28%. In many instances the pathophysiologic processes were of such a nature that current medical expertise has not reached a level of development to effectively cope with the situation. There were, however, a significant number of cases where survival may have been achieved had it not been for deficiences on the part of the patient, the primary health care personnel or those in attendence at the referral center. The basic keystone for a successful outcome in the management of patients with the gangrenous bowel problem is early surgical intervention. All will be lost if patient exposure to this source of lethal toxins is allowed to proceed to an irreversible stage. Liberal antibiotic administration probably postpones the arrival of intractable hypotension. Other factors which can be expected to improve the survival rate include minimization of technical errors, repair of incidental hernias, elemination of dependence upon nasogastric tubes for the definitive management of patients with complete bowel obstruction (with one or two exceptions), and a firm commitment to the diligent pursuit and early definitive management of postoperative complications.", "contents": "A current appraisal of problems with gangrenous bowel. Gangrenous bowel most often results from hernia, adhesions and mesenteric insufficiency. The overall mortality rate for 151 cases was 37%. This figure was 20% for hernia, 23% for adhesions and 74% for mesenteric insufficiency. In the latter category where bowel resection was feasable the mortality rate was 40%. Other causes of bowel gangrene had a mortality rate of 28%. In many instances the pathophysiologic processes were of such a nature that current medical expertise has not reached a level of development to effectively cope with the situation. There were, however, a significant number of cases where survival may have been achieved had it not been for deficiences on the part of the patient, the primary health care personnel or those in attendence at the referral center. The basic keystone for a successful outcome in the management of patients with the gangrenous bowel problem is early surgical intervention. All will be lost if patient exposure to this source of lethal toxins is allowed to proceed to an irreversible stage. Liberal antibiotic administration probably postpones the arrival of intractable hypotension. Other factors which can be expected to improve the survival rate include minimization of technical errors, repair of incidental hernias, elemination of dependence upon nasogastric tubes for the definitive management of patients with complete bowel obstruction (with one or two exceptions), and a firm commitment to the diligent pursuit and early definitive management of postoperative complications.", "PMID": 973752}
{"id": "pubmed23n0032_9478", "title": "[Lipids in Taenia saginata].", "content": "Lipids of Taenia saginata were analyzed by means of thin layer chromatography and gas liquid chromatography. Total lipids comprised about 3.3% of the fresh weight and consisted mainly of triglycerides (65.62%), sterols (19.58%), phospholipids (10.16%). The fatty acids of the triglycerides were mainly unsaturated and differed from those of phospholipids. The parasite has practically no \"de novo\" fatty acid biosynthesis, although this cestode did synthetize complex lipids from single lipids provided by the diet.", "contents": "[Lipids in Taenia saginata]. Lipids of Taenia saginata were analyzed by means of thin layer chromatography and gas liquid chromatography. Total lipids comprised about 3.3% of the fresh weight and consisted mainly of triglycerides (65.62%), sterols (19.58%), phospholipids (10.16%). The fatty acids of the triglycerides were mainly unsaturated and differed from those of phospholipids. The parasite has practically no \"de novo\" fatty acid biosynthesis, although this cestode did synthetize complex lipids from single lipids provided by the diet.", "PMID": 973747}
{"id": "pubmed23n0032_9479", "title": "Use of aminoglycosides in surgical infections.", "content": "Most infections on the surgical ward are due to one or more gram-negative rods, acting either as the sole pathogens or as principal components in a polymicrobial flora. To date, parenteral aminoglycosides have proven to be the most effective antibiotics for control or treatment of such sepsis. Unfortunately, however, serious complications as well as therapeutic failures do occur. During a 40-month period, 405 surgical patients receiving aminoglycosides (Gentamicin, Tobramycin, Sisomicin, or Amikacin) were prospectively studied with respect to: indications for antibiotic; patient population; serum concentrations of antibiotic according to route of administration, dose in mg/kg/day, and renal function; rapidity of antibiotic excretion in the urine; causative bacteria and their sensitivities to each aminoglycoside as determined by both disc and tube dilution methods; severity and frequency of drug complications; and clinical efficacy of each study antibiotic. Results supported the contention of a superior effectiveness from aminoglycosides for established abdominal and unspecified surgical infections, more rapid development of therapeutic blood levels by intravenous administration, need to alter drug dose according to frequent serum creatinine determinations, increased drug toxicity in dehydrated and shocked patients, preventability of complicating Candida sepsis, and the importance of early as well as adequate surgical debridement and drainage.", "contents": "Use of aminoglycosides in surgical infections. Most infections on the surgical ward are due to one or more gram-negative rods, acting either as the sole pathogens or as principal components in a polymicrobial flora. To date, parenteral aminoglycosides have proven to be the most effective antibiotics for control or treatment of such sepsis. Unfortunately, however, serious complications as well as therapeutic failures do occur. During a 40-month period, 405 surgical patients receiving aminoglycosides (Gentamicin, Tobramycin, Sisomicin, or Amikacin) were prospectively studied with respect to: indications for antibiotic; patient population; serum concentrations of antibiotic according to route of administration, dose in mg/kg/day, and renal function; rapidity of antibiotic excretion in the urine; causative bacteria and their sensitivities to each aminoglycoside as determined by both disc and tube dilution methods; severity and frequency of drug complications; and clinical efficacy of each study antibiotic. Results supported the contention of a superior effectiveness from aminoglycosides for established abdominal and unspecified surgical infections, more rapid development of therapeutic blood levels by intravenous administration, need to alter drug dose according to frequent serum creatinine determinations, increased drug toxicity in dehydrated and shocked patients, preventability of complicating Candida sepsis, and the importance of early as well as adequate surgical debridement and drainage.", "PMID": 973753}
{"id": "pubmed23n0032_9480", "title": "[Detection of anti-Toxoplasma gondii antibodies in subjects with repeated abortions, perinatal mortality and malformed infants].", "content": "The AA. investigated the frequency of antibodies against Toxoplasma gondii in 426 women with repeated abortions, perinatal mortality and malformed newborns. They showed on the whole a low incidence even if they demonstrated a high incidence (77.7%) of antibodies in women with malformed newborns. The meaning of the observations are briefly discussed.", "contents": "[Detection of anti-Toxoplasma gondii antibodies in subjects with repeated abortions, perinatal mortality and malformed infants]. The AA. investigated the frequency of antibodies against Toxoplasma gondii in 426 women with repeated abortions, perinatal mortality and malformed newborns. They showed on the whole a low incidence even if they demonstrated a high incidence (77.7%) of antibodies in women with malformed newborns. The meaning of the observations are briefly discussed.", "PMID": 973746}
{"id": "pubmed23n0032_9481", "title": "Diagnosis and management of blunt abdominal trauma.", "content": "The records of 437 patients with blunt abdominal trauma admitted to Charity Hospital, New Orleans, from 1967-1973 have been reviewed and computer-analyzed. There was an 80% increase in the incidence of blunt abdominal trauma when compared with the preceding 15-year experience. Forty-three per cent of all the patients presented with no specific complaint or sign of injury. Blunt abdominal injury was usually diagnosed preoperatively using conventional methods including history, physical examination, and routine laboratory tests and x-rays. Abdominal paracentesis via a Potter needle had an 86% accuracy. The incidence and management of specific organ injuries with associated morbidity and mortality have been discussed. Mortality and morbidity continue to be significant in blunt abdominal trauma. Isolated abdominal injuries rarely (5%) resulted in death, even though abdominal injuries accounted for 41% of all deaths. Associated injuries, especially head injury, greatly increased the risk. The insidious nature of blunt abdominal injury is borne out by the fact that more than one-third of the \"asymptomatic\" patients had an abdominal organ injured. A high index of suspicion and an adequate observation period therefore are mandatory for proper care of patients subjected to blunt trauma.", "contents": "Diagnosis and management of blunt abdominal trauma. The records of 437 patients with blunt abdominal trauma admitted to Charity Hospital, New Orleans, from 1967-1973 have been reviewed and computer-analyzed. There was an 80% increase in the incidence of blunt abdominal trauma when compared with the preceding 15-year experience. Forty-three per cent of all the patients presented with no specific complaint or sign of injury. Blunt abdominal injury was usually diagnosed preoperatively using conventional methods including history, physical examination, and routine laboratory tests and x-rays. Abdominal paracentesis via a Potter needle had an 86% accuracy. The incidence and management of specific organ injuries with associated morbidity and mortality have been discussed. Mortality and morbidity continue to be significant in blunt abdominal trauma. Isolated abdominal injuries rarely (5%) resulted in death, even though abdominal injuries accounted for 41% of all deaths. Associated injuries, especially head injury, greatly increased the risk. The insidious nature of blunt abdominal injury is borne out by the fact that more than one-third of the \"asymptomatic\" patients had an abdominal organ injured. A high index of suspicion and an adequate observation period therefore are mandatory for proper care of patients subjected to blunt trauma.", "PMID": 973754}
{"id": "pubmed23n0032_9482", "title": "The healing of liver wounds.", "content": "Cases of traumatic hemobilia are often characterized by a protracted history with recurrent episodes of hemorrhage over many years. It seems that while the liver regenerates quickly and profusely it heals slowly and poorly. This is in contradistinction to the kidneys where lesions often heal within a short time. In order to investigate the cause of this peculiar behavior a series of experiments was performed where local lesions were produced in the musculature, in the kidneys and in the liver of dogs. The healing process was studied at different intervals. There was essentially no difference between the rate and character of healing in the three localities. As the lesions that produce hemobilia are open to the biliary tract, another series of experiments was performed where the healing took place in presence of bile. The effect of this was striking with a very diminished production of fibrinous exudate, granulating tissue and fibrous scar.", "contents": "The healing of liver wounds. Cases of traumatic hemobilia are often characterized by a protracted history with recurrent episodes of hemorrhage over many years. It seems that while the liver regenerates quickly and profusely it heals slowly and poorly. This is in contradistinction to the kidneys where lesions often heal within a short time. In order to investigate the cause of this peculiar behavior a series of experiments was performed where local lesions were produced in the musculature, in the kidneys and in the liver of dogs. The healing process was studied at different intervals. There was essentially no difference between the rate and character of healing in the three localities. As the lesions that produce hemobilia are open to the biliary tract, another series of experiments was performed where the healing took place in presence of bile. The effect of this was striking with a very diminished production of fibrinous exudate, granulating tissue and fibrous scar.", "PMID": 973755}
{"id": "pubmed23n0032_9483", "title": "Selective operative treatment for tetraology of Fallot: rationale and results.", "content": "Eighty-one patients with tetralogy of Fallot malformations evaluated between July 1, 1971 and November, 1975 are presented. Fifty-one patients underwent primary intracardiac repair; three died. Twenty-seven patients were corrected after a previous palliative shunt; there was one death. Three additional patients have been palliated and are awaiting repair. While the overall mortality in these patients was less than 5%, the infants undergoing total correction before the age of two years appeared to be at greater risk (25%). There were no deaths in the group of patients undergoing palliative procedures. Based on these data it appears that a safely performed palliative shunt in the symptomatic small infant is a reasonable first step, particularly if the outflow tract of the right ventricle is diffusely hypoplastic.", "contents": "Selective operative treatment for tetraology of Fallot: rationale and results. Eighty-one patients with tetralogy of Fallot malformations evaluated between July 1, 1971 and November, 1975 are presented. Fifty-one patients underwent primary intracardiac repair; three died. Twenty-seven patients were corrected after a previous palliative shunt; there was one death. Three additional patients have been palliated and are awaiting repair. While the overall mortality in these patients was less than 5%, the infants undergoing total correction before the age of two years appeared to be at greater risk (25%). There were no deaths in the group of patients undergoing palliative procedures. Based on these data it appears that a safely performed palliative shunt in the symptomatic small infant is a reasonable first step, particularly if the outflow tract of the right ventricle is diffusely hypoplastic.", "PMID": 973756}
{"id": "pubmed23n0032_9484", "title": "Peripheral arterial injuries: a reassessment.", "content": "Ninety-four patients with peripheral arterial injuries were subjected to acute repair, negative exploration, or late repair of the complications of the arterial injury (false aneurysm, A-V fistula, and/or limb ischemia). The causes of failure after acute injury include extensive local soft tissue and bony damage, severe concomitant head, chest or abdominal wounding, stubborn reliance on negative arteriograms in patients with probable arterial injury, failure to repair simultaneous venous injuries, or harvesting of a vein graft from a severely damaged extremity. There is a positive correlation between non-operative expectant treatment and the incidence of late vascular complications requiring late arterial repair. Delayed complications of arterial injuries occurred most frequently in wounds below the elbow and knee.", "contents": "Peripheral arterial injuries: a reassessment. Ninety-four patients with peripheral arterial injuries were subjected to acute repair, negative exploration, or late repair of the complications of the arterial injury (false aneurysm, A-V fistula, and/or limb ischemia). The causes of failure after acute injury include extensive local soft tissue and bony damage, severe concomitant head, chest or abdominal wounding, stubborn reliance on negative arteriograms in patients with probable arterial injury, failure to repair simultaneous venous injuries, or harvesting of a vein graft from a severely damaged extremity. There is a positive correlation between non-operative expectant treatment and the incidence of late vascular complications requiring late arterial repair. Delayed complications of arterial injuries occurred most frequently in wounds below the elbow and knee.", "PMID": 973757}
{"id": "pubmed23n0032_9485", "title": "Tibial and peroneal bypass in severe occlusive disease of the lower extremities.", "content": "Symptomatic arteriosclerotic occlusive disease involving the femoral and proximal popliteal arteries is currently best treated by reversed autogenous saphenous vein bypass graft. Severe occlusive disease frequently includes the popliteal and/or origin of the trifurcation vessels with reconstitution of one or more of the vessels in the lower leg. We have used distal bypass 97 times in 90 patients during the past decade. There was only one postoperative death in the series in spite of the advanced age and concurrent disease in the majority  of the patients. Our indications for operation continue to be relief of pain or salvage of an extremity. We rely on high quality preoperative angiograms for selection of vessels to receive the bypass. The posterior tibial artery was used in 63 of the cases, while the anterior tibial and peroneal were used in 24 and 10 cases, respectively. There were 11 immediate inhospital failures in this series of 97 cases requiring amputation in five. Three additional patients had amputations during the ensuing several months. Of the 85 grafts functioning at the time of discharge from the hospital, 16 or (18.8%) failed during the first years. Grafts that remained patent for one year have a high incidence of long term patency which is in keeping with other reported series.", "contents": "Tibial and peroneal bypass in severe occlusive disease of the lower extremities. Symptomatic arteriosclerotic occlusive disease involving the femoral and proximal popliteal arteries is currently best treated by reversed autogenous saphenous vein bypass graft. Severe occlusive disease frequently includes the popliteal and/or origin of the trifurcation vessels with reconstitution of one or more of the vessels in the lower leg. We have used distal bypass 97 times in 90 patients during the past decade. There was only one postoperative death in the series in spite of the advanced age and concurrent disease in the majority  of the patients. Our indications for operation continue to be relief of pain or salvage of an extremity. We rely on high quality preoperative angiograms for selection of vessels to receive the bypass. The posterior tibial artery was used in 63 of the cases, while the anterior tibial and peroneal were used in 24 and 10 cases, respectively. There were 11 immediate inhospital failures in this series of 97 cases requiring amputation in five. Three additional patients had amputations during the ensuing several months. Of the 85 grafts functioning at the time of discharge from the hospital, 16 or (18.8%) failed during the first years. Grafts that remained patent for one year have a high incidence of long term patency which is in keeping with other reported series.", "PMID": 973758}
{"id": "pubmed23n0032_9486", "title": "Pneumonectomy: review of 351 cases.", "content": "Among 351 patients undergoing pneumonectomy, the mortality was 6%, and when the operation was performed for malignant disease, the mortality was 8.5%. Much of the risk of the operation is associated with the age, physiologic state, and associated diseases which are prevalent in patients who require pneumonectomy. The conduct of the operative procedure itself is of paramount importance since complications clearly increase the mortality. Operative mishaps predispose to postoperative complications, and the addition of postoperative problems to the operative complications combine to bring the mortality to 24%. Careful hemostasis, meticulous technique, fluid and blood replacement, as well as careful preoperative and postoperative management combine to produce an acceptable mortality in this serious procedure.", "contents": "Pneumonectomy: review of 351 cases. Among 351 patients undergoing pneumonectomy, the mortality was 6%, and when the operation was performed for malignant disease, the mortality was 8.5%. Much of the risk of the operation is associated with the age, physiologic state, and associated diseases which are prevalent in patients who require pneumonectomy. The conduct of the operative procedure itself is of paramount importance since complications clearly increase the mortality. Operative mishaps predispose to postoperative complications, and the addition of postoperative problems to the operative complications combine to bring the mortality to 24%. Careful hemostasis, meticulous technique, fluid and blood replacement, as well as careful preoperative and postoperative management combine to produce an acceptable mortality in this serious procedure.", "PMID": 973759}
{"id": "pubmed23n0032_9487", "title": "Repair of inflammatory tracheoesophageal fistula.", "content": "Benign acquired tracheoesophageal fistula is uncommon. Erosin of the membranous wall of the trachea and the anterior esophageal wall by the high-pressure cuff on a tracheostomy tube, often against the anvil of a nasogastric tube, may produce such fistulas. Techniques for closure have included patching the tracheal defect with muscle and, often, multiple staged procedures, planned or unplanned. Since any cuff lesion severe enough to cause a fistula necessarily damages the trachea circumferentially at the same level, definitive correction must include circumferential tracheal resection as well as closure of the fitstula. Five patients with tracheoesophageal fistula due to cuff perforation had repair by such a single-stage procedure. Through an anterior approach the involved trachea was resected, primary anastomosis was done, and the esophagus was closed in layers. In 3 of these 5 patients muscle was interposed for added security. One patient had undergone a prior attempt at repair elsewhere. One required a second resection of trachea for subsequent stomal stenosis. Repair in 2 additional patients with fistulas of complex origin related to direct trauma, sepsis, and foreign body involved adaptation of the basic technique to the special problem; 1 of these procedures was necessarily staged. Results in all 7 patients have been good.", "contents": "Repair of inflammatory tracheoesophageal fistula. Benign acquired tracheoesophageal fistula is uncommon. Erosin of the membranous wall of the trachea and the anterior esophageal wall by the high-pressure cuff on a tracheostomy tube, often against the anvil of a nasogastric tube, may produce such fistulas. Techniques for closure have included patching the tracheal defect with muscle and, often, multiple staged procedures, planned or unplanned. Since any cuff lesion severe enough to cause a fistula necessarily damages the trachea circumferentially at the same level, definitive correction must include circumferential tracheal resection as well as closure of the fitstula. Five patients with tracheoesophageal fistula due to cuff perforation had repair by such a single-stage procedure. Through an anterior approach the involved trachea was resected, primary anastomosis was done, and the esophagus was closed in layers. In 3 of these 5 patients muscle was interposed for added security. One patient had undergone a prior attempt at repair elsewhere. One required a second resection of trachea for subsequent stomal stenosis. Repair in 2 additional patients with fistulas of complex origin related to direct trauma, sepsis, and foreign body involved adaptation of the basic technique to the special problem; 1 of these procedures was necessarily staged. Results in all 7 patients have been good.", "PMID": 973760}
{"id": "pubmed23n0032_9488", "title": "Interruption of patent ductus arteriosus in premature infants with respiratory distress syndrome.", "content": "In infants with respiratory distress syndrome (RDS) hypoxemia inhibits closure of the patent ductus arteriosus (PDA), resulting in increased pulmonary blood flow with subsequent increased hypoxemia. In an attempt to interrupt this cycle 42 consecutive premature infants with RDS and PDA, weighing between 550 and 2,000 gm (average, 1,383 gm) and with an average gestational age of 31 weeks, were arbitrarily treated either medically (13 patients) or by interruption of the PDA (20 patients). Eleven patients who were initially treated medically could not be weaned from the respirator and later underwent operation. There were no operative or anesthetic deaths; late survival was 65% (20 patients). The last 31 patients were randomly divided into operative and nonoperative groups. Preliminary results revealed no significant differences in late survival between the two groups. Since the operative risk is minimal, further investigative efforts are indicated to settle this issue.", "contents": "Interruption of patent ductus arteriosus in premature infants with respiratory distress syndrome. In infants with respiratory distress syndrome (RDS) hypoxemia inhibits closure of the patent ductus arteriosus (PDA), resulting in increased pulmonary blood flow with subsequent increased hypoxemia. In an attempt to interrupt this cycle 42 consecutive premature infants with RDS and PDA, weighing between 550 and 2,000 gm (average, 1,383 gm) and with an average gestational age of 31 weeks, were arbitrarily treated either medically (13 patients) or by interruption of the PDA (20 patients). Eleven patients who were initially treated medically could not be weaned from the respirator and later underwent operation. There were no operative or anesthetic deaths; late survival was 65% (20 patients). The last 31 patients were randomly divided into operative and nonoperative groups. Preliminary results revealed no significant differences in late survival between the two groups. Since the operative risk is minimal, further investigative efforts are indicated to settle this issue.", "PMID": 973761}
{"id": "pubmed23n0032_9489", "title": "Patent ductus arteriosus ligation and respiratory distress syndrome in premature infants.", "content": "Ligation of a patent ductus arteriosus was carried out in 22 premature infants, 20 with concomitant respiratory distress. The duration of high-volume shunting is critical in determining the prognosis for these infants. Because of the low surgical mortality and morbidity and the high incidence of bronchopulmonary dysplasia in babies managed conservatively, infants with respiratory distress syndrome (RDS) who are respirator dependent should undergo ligation as soon as the presence of large left-to-right shunting is detetmined. Premature infants without RDS or those with mild RDS who are not respirator dependent can be managed medically or with elective ligation. Surgical intervention is strongly indicated in patients with persistent congestive heart failure and respiratory failure. Echocardiography offers an accurate and risk-free approach to the early diagnosis of a large left-to-right shunt through the ductus.", "contents": "Patent ductus arteriosus ligation and respiratory distress syndrome in premature infants. Ligation of a patent ductus arteriosus was carried out in 22 premature infants, 20 with concomitant respiratory distress. The duration of high-volume shunting is critical in determining the prognosis for these infants. Because of the low surgical mortality and morbidity and the high incidence of bronchopulmonary dysplasia in babies managed conservatively, infants with respiratory distress syndrome (RDS) who are respirator dependent should undergo ligation as soon as the presence of large left-to-right shunting is detetmined. Premature infants without RDS or those with mild RDS who are not respirator dependent can be managed medically or with elective ligation. Surgical intervention is strongly indicated in patients with persistent congestive heart failure and respiratory failure. Echocardiography offers an accurate and risk-free approach to the early diagnosis of a large left-to-right shunt through the ductus.", "PMID": 973762}
{"id": "pubmed23n0032_9490", "title": "The ductus debate: ligation in prematurity?", "content": "This report describes 20 premature infants who have undergone surgical ligation of a patent ductus arteriosus (PDA), diagnosed solely by physical examination in all but 2 instances. There were no operative deaths. Eleven of these 20 infants had severe associated hyaline membrane disease (HMD) necessitating mechanical ventilatory support from the first or second day of life. Only 3 of these 11 infants (27%) ultimately survived to leave the hospital. In contrast, 8 of 9 premature meonates (89%) with severe congestive heart failure but without HMD were discharged alive. Surgical ligation of the PDA in a premature infant is an appropriate and successful mode of treatment when congestive heart failure is refractory to medical management. Proof is lacking, however, to establish the efficacy of PDA ligation in the premature infant with HMD.", "contents": "The ductus debate: ligation in prematurity? This report describes 20 premature infants who have undergone surgical ligation of a patent ductus arteriosus (PDA), diagnosed solely by physical examination in all but 2 instances. There were no operative deaths. Eleven of these 20 infants had severe associated hyaline membrane disease (HMD) necessitating mechanical ventilatory support from the first or second day of life. Only 3 of these 11 infants (27%) ultimately survived to leave the hospital. In contrast, 8 of 9 premature meonates (89%) with severe congestive heart failure but without HMD were discharged alive. Surgical ligation of the PDA in a premature infant is an appropriate and successful mode of treatment when congestive heart failure is refractory to medical management. Proof is lacking, however, to establish the efficacy of PDA ligation in the premature infant with HMD.", "PMID": 973764}
{"id": "pubmed23n0032_9491", "title": "Outpatient management of intercostal tube drainage in spontaneous pneumothorax.", "content": "In a series of 226 patients with spontaneous pneumothorax, 167 (74%) were managed successfully on an outpatient basis by observation (20%) or by intercostal tube drainage and a flutter valve (54%). Only 59 patients (26%) were hospitalized, and 42 of these were treated surgically (18.6%). Outpatient management with a flutter valve has proved to be safe, efficient, and economical.", "contents": "Outpatient management of intercostal tube drainage in spontaneous pneumothorax. In a series of 226 patients with spontaneous pneumothorax, 167 (74%) were managed successfully on an outpatient basis by observation (20%) or by intercostal tube drainage and a flutter valve (54%). Only 59 patients (26%) were hospitalized, and 42 of these were treated surgically (18.6%). Outpatient management with a flutter valve has proved to be safe, efficient, and economical.", "PMID": 973765}
{"id": "pubmed23n0032_9492", "title": "Cardiac performance and oxygen consumption during intracardiac operations in children.", "content": "Cardiac performance and oxygen consumption in 30 patients undergoing surgical treatment for congenital cardiac disease were determined from intraoperative measurements. Arterial pressure, cardiac index, mean left ventricular hydraulic output power, pulmonary artery oxygen saturation were obtained at average mean left atrial pressures of 8.1 and 15.2 cm H2O after cardiopulmonary bypass in 20 patients. These same variables were measured at an average pulmonary artery saturation of 65 and 75% in 30 patients. A composite of measurements of cardiac performance was desirable to guide precise patient care intraoperatively, especially if myocardial function was compromised. It is suggested that left atrial pressure be maintained at a level that results in a pulmonary artery saturation greater than 65%.", "contents": "Cardiac performance and oxygen consumption during intracardiac operations in children. Cardiac performance and oxygen consumption in 30 patients undergoing surgical treatment for congenital cardiac disease were determined from intraoperative measurements. Arterial pressure, cardiac index, mean left ventricular hydraulic output power, pulmonary artery oxygen saturation were obtained at average mean left atrial pressures of 8.1 and 15.2 cm H2O after cardiopulmonary bypass in 20 patients. These same variables were measured at an average pulmonary artery saturation of 65 and 75% in 30 patients. A composite of measurements of cardiac performance was desirable to guide precise patient care intraoperatively, especially if myocardial function was compromised. It is suggested that left atrial pressure be maintained at a level that results in a pulmonary artery saturation greater than 65%.", "PMID": 973766}
{"id": "pubmed23n0032_9493", "title": "The effects of sterilization and storage treatments on the stress-strain behavior of aortic valve leaflets.", "content": "An investigation was made of the feasibility of using tensile tests to detect alterations in the molecular structure of aortic valve tissue brought about by sterilizing or storage treatments. Stress-strain tests were carried out on fresh valve leaflets from pigs and repeated with the same specimens after specified sterilization or storage treatments. It was found that the stress at a given strain was increased by treatment with ethylene oxide, buffered formalin, and tissue culture solution and decreased by treatment with antibiotics. The least change was with tissue culture solution and antibiotics. It has been suggested that the best method of allograft sterilization should involve minimal structural alteration to the collagen and elastic  components of the tissue. Thus, treatment with tissue culture solution and antibiotics seems appropriate.", "contents": "The effects of sterilization and storage treatments on the stress-strain behavior of aortic valve leaflets. An investigation was made of the feasibility of using tensile tests to detect alterations in the molecular structure of aortic valve tissue brought about by sterilizing or storage treatments. Stress-strain tests were carried out on fresh valve leaflets from pigs and repeated with the same specimens after specified sterilization or storage treatments. It was found that the stress at a given strain was increased by treatment with ethylene oxide, buffered formalin, and tissue culture solution and decreased by treatment with antibiotics. The least change was with tissue culture solution and antibiotics. It has been suggested that the best method of allograft sterilization should involve minimal structural alteration to the collagen and elastic  components of the tissue. Thus, treatment with tissue culture solution and antibiotics seems appropriate.", "PMID": 973767}
{"id": "pubmed23n0032_9494", "title": "Carbon monoxide accumulation during extracoporeal membrane oxygenation for acute respiratory failure.", "content": "A marked increase in the carbon monoxide level in the blood sufficient to interfere with oxygen binding of hemoglobin was observed in a 43-year-old man during the course of extracorporeal membrane oxygenator support for acute respiratory failure from viral pneumonitis. The increased carbon monoxide level in this man was temporally related to the transfusion of large amounts of old bank blood. The etiology of an increased level of carbon monoxide in the blood during extracorporeal circulation is discussed and solutions to this problem are suggested.", "contents": "Carbon monoxide accumulation during extracoporeal membrane oxygenation for acute respiratory failure. A marked increase in the carbon monoxide level in the blood sufficient to interfere with oxygen binding of hemoglobin was observed in a 43-year-old man during the course of extracorporeal membrane oxygenator support for acute respiratory failure from viral pneumonitis. The increased carbon monoxide level in this man was temporally related to the transfusion of large amounts of old bank blood. The etiology of an increased level of carbon monoxide in the blood during extracorporeal circulation is discussed and solutions to this problem are suggested.", "PMID": 973768}
{"id": "pubmed23n0032_9495", "title": "[Value of fibrinogen level measurement in neonatal infections due to maternal  contamination].", "content": "Fibrinogen, factors V, II and VII + X assays have been performed by micromethod on day 1, 2 and 3 in 275 newborn infants (full-term and premature) over a period of one year -- 20 infants had proved bacterial infection (from maternal contamination) with positive cultures. The control group included 20 normal newborns in the same range of gestational age. The increase of the fibrinogen level in the infected infants appeared to be significant on day 1 and 2 but not on day 3 (Day 1: 4,06 g/l +/- 0,74 g/l versus 2,06 g/l +/- 0,28 g/l in normal newborn. p less than 0.0001; on Day 2: 4,20 +/- 0,86 g/l versus 2,32 g/l +/- 0,09 g/l p less than 0.001; on Day 3: 3,68 g/l +/- 0,62 versus 3,36 +/- 0,66 g/l). The level of fibrinogen measured in the newborn period by the micromethod was in a accordance with former works showing that it is up th the adult value after the 3rd day of life. Since the micromethod on capillary blood requires only 140 microliters of blood (whereas 2 ml with macromethod) this fibrinogen determination appears to be a rapid and reliable method for the diagnosis of neonatal infection due to materno-fetal contamination.", "contents": "[Value of fibrinogen level measurement in neonatal infections due to maternal  contamination]. Fibrinogen, factors V, II and VII + X assays have been performed by micromethod on day 1, 2 and 3 in 275 newborn infants (full-term and premature) over a period of one year -- 20 infants had proved bacterial infection (from maternal contamination) with positive cultures. The control group included 20 normal newborns in the same range of gestational age. The increase of the fibrinogen level in the infected infants appeared to be significant on day 1 and 2 but not on day 3 (Day 1: 4,06 g/l +/- 0,74 g/l versus 2,06 g/l +/- 0,28 g/l in normal newborn. p less than 0.0001; on Day 2: 4,20 +/- 0,86 g/l versus 2,32 g/l +/- 0,09 g/l p less than 0.001; on Day 3: 3,68 g/l +/- 0,62 versus 3,36 +/- 0,66 g/l). The level of fibrinogen measured in the newborn period by the micromethod was in a accordance with former works showing that it is up th the adult value after the 3rd day of life. Since the micromethod on capillary blood requires only 140 microliters of blood (whereas 2 ml with macromethod) this fibrinogen determination appears to be a rapid and reliable method for the diagnosis of neonatal infection due to materno-fetal contamination.", "PMID": 973771}
{"id": "pubmed23n0032_9496", "title": "[Kniest's disease, (a familial case)].", "content": "Kniest's disease (spondylo-epiphyseal dysplasia) was recently described by Maroteaux and Spranger. A second familial case is studied. Transmission of the disease occurred as a dominant trait.", "contents": "[Kniest's disease, (a familial case)]. Kniest's disease (spondylo-epiphyseal dysplasia) was recently described by Maroteaux and Spranger. A second familial case is studied. Transmission of the disease occurred as a dominant trait.", "PMID": 973772}
{"id": "pubmed23n0032_9497", "title": "[Infantile and juvenile hypothyroidism with the gland in place and low radio-iodide uptake].", "content": "27 hypothyroid infants of children, with a low iodine uptake in spite of a thyroid gland or thyroid tissue in normal pretracheal place, have been studied. 21 cases are related to primary thyroid involvement leading to vanishing iodine or technetium uptake. In 8 of these cases, clinical onset in late childhood, high frequency of antithyroid antibodies and of familial thyroid dysfunction were similar to those found in childhood's thyroiditis. 8 other cases had a precocious onset (first months in 6, first year in 1, second year in 1) with some pretracheal iodine uptake when first studied and no uptake at further examinations, 1 patient having received no treatment from first to second study, the others being without treatment from more than two months and certain of them receiving injections of TSH. The last 5 cases of this group were those of children born to 2 mothers with treated hypothyroidism, with low iodine uptake in pretracheal place. The role of genetic factors and auto-immunity in theses cases is discussed. The 6 other patients had clinically isolated hypothyroidism secondary to TSH deficiency, eventually associated to clinically inapparent GH or ACTH deficiencies, most of them by hypothalamic defect with normal response to TRH.", "contents": "[Infantile and juvenile hypothyroidism with the gland in place and low radio-iodide uptake]. 27 hypothyroid infants of children, with a low iodine uptake in spite of a thyroid gland or thyroid tissue in normal pretracheal place, have been studied. 21 cases are related to primary thyroid involvement leading to vanishing iodine or technetium uptake. In 8 of these cases, clinical onset in late childhood, high frequency of antithyroid antibodies and of familial thyroid dysfunction were similar to those found in childhood's thyroiditis. 8 other cases had a precocious onset (first months in 6, first year in 1, second year in 1) with some pretracheal iodine uptake when first studied and no uptake at further examinations, 1 patient having received no treatment from first to second study, the others being without treatment from more than two months and certain of them receiving injections of TSH. The last 5 cases of this group were those of children born to 2 mothers with treated hypothyroidism, with low iodine uptake in pretracheal place. The role of genetic factors and auto-immunity in theses cases is discussed. The 6 other patients had clinically isolated hypothyroidism secondary to TSH deficiency, eventually associated to clinically inapparent GH or ACTH deficiencies, most of them by hypothalamic defect with normal response to TRH.", "PMID": 973777}
{"id": "pubmed23n0032_9498", "title": "[Stimulation by the propranolol-glucagon test of somatotropin secretion in 71 children. Results, statistical study and comparison with the insulin-arginine test].", "content": "Two tests of stimulation: insulin + arginine and propranolol + glucagon were successively performed in 62 children who were either normal or had essential growth retardation. Average peak value was 10.6 +/- 1.1 ng/ml in the first test and 22.8 +/- 1.4 ng/ml in the second. In 56 cases the response obtained with propranolol + glucagon was higher than that obtained with insulin + arginine. Twenty-four false negative results were obtained employing insulin + arginine, stimulation by propranolol + glucagon resulting in normal values. The determination of the confidence interval at 95% and of the 3rd percentile did not allow to establish the lower threshold for the insulin + arginine test. For the propranolol + glucagon test 7.6 ng/ml for the interval at 95% and 8 ng/ml for 3rd percentile were found as minimal threshold. Therefore, a response below 8 ng/ml should be considered as pathological with the latter test.", "contents": "[Stimulation by the propranolol-glucagon test of somatotropin secretion in 71 children. Results, statistical study and comparison with the insulin-arginine test]. Two tests of stimulation: insulin + arginine and propranolol + glucagon were successively performed in 62 children who were either normal or had essential growth retardation. Average peak value was 10.6 +/- 1.1 ng/ml in the first test and 22.8 +/- 1.4 ng/ml in the second. In 56 cases the response obtained with propranolol + glucagon was higher than that obtained with insulin + arginine. Twenty-four false negative results were obtained employing insulin + arginine, stimulation by propranolol + glucagon resulting in normal values. The determination of the confidence interval at 95% and of the 3rd percentile did not allow to establish the lower threshold for the insulin + arginine test. For the propranolol + glucagon test 7.6 ng/ml for the interval at 95% and 8 ng/ml for 3rd percentile were found as minimal threshold. Therefore, a response below 8 ng/ml should be considered as pathological with the latter test.", "PMID": 973778}
{"id": "pubmed23n0032_9499", "title": "[Incidence of mother-child contamination by Candida albicans during labor].", "content": "Two different studies assessed that materno-fetal contamination at time of delivery is not the usual way of colonization of newborns with Candida albicans. In a first study, occurence of Candida albicans in neonate units disappeared owing to prophylactic measures, such as strict care in hospital hygiene. In a second study, detailed herein and performed in an obstetric ward, it was found that despite a rate of vaginal Candida carrier of 19 %, candidiasis in newborns was exceptional.", "contents": "[Incidence of mother-child contamination by Candida albicans during labor]. Two different studies assessed that materno-fetal contamination at time of delivery is not the usual way of colonization of newborns with Candida albicans. In a first study, occurence of Candida albicans in neonate units disappeared owing to prophylactic measures, such as strict care in hospital hygiene. In a second study, detailed herein and performed in an obstetric ward, it was found that despite a rate of vaginal Candida carrier of 19 %, candidiasis in newborns was exceptional.", "PMID": 973779}
{"id": "pubmed23n0032_9500", "title": "[Salt-losing syndrome in newborn infants with hyperaldosteronism. Presenting a sensitivity to mineralocorticoids. Pseudohypoaldosteronism].", "content": "A case of salt-loosing syndrome in infancy with hyperaldosteronism is reported. This patient was given high doses of 9 alpha Fluorocortisol (0.1 mg/kg/24 h), together with salt supplements. This treatment corrected the abnormality, while higher doses of salt supplements without such large amounts of Flurocortisol were ineffective.", "contents": "[Salt-losing syndrome in newborn infants with hyperaldosteronism. Presenting a sensitivity to mineralocorticoids. Pseudohypoaldosteronism]. A case of salt-loosing syndrome in infancy with hyperaldosteronism is reported. This patient was given high doses of 9 alpha Fluorocortisol (0.1 mg/kg/24 h), together with salt supplements. This treatment corrected the abnormality, while higher doses of salt supplements without such large amounts of Flurocortisol were ineffective.", "PMID": 973780}
{"id": "pubmed23n0032_9501", "title": "[Hemophagocytic reticulosis with hypertriglyceridemia].", "content": "This syndrome is characterized by a hyper-triglyceridemia associated with a deficiency of the plasma lipolytic activity induced by heparin infusion. All signes ascribed to familial lymphohistocytosis were found in this patient. When a diagnosis of acute reticulosis in children is made, such biological characteristics should be searched for. Since in systemic lupus erythematosus an acquired and analogous type of hyperlipidemia has been described, the hypothesis of an immunological pathogenesis in familial lymphohistiocytosis may be plausible.", "contents": "[Hemophagocytic reticulosis with hypertriglyceridemia]. This syndrome is characterized by a hyper-triglyceridemia associated with a deficiency of the plasma lipolytic activity induced by heparin infusion. All signes ascribed to familial lymphohistocytosis were found in this patient. When a diagnosis of acute reticulosis in children is made, such biological characteristics should be searched for. Since in systemic lupus erythematosus an acquired and analogous type of hyperlipidemia has been described, the hypothesis of an immunological pathogenesis in familial lymphohistiocytosis may be plausible.", "PMID": 973781}
{"id": "pubmed23n0032_9502", "title": "An electron microscopic study of the ciliated cells in the human gastric mucosa.", "content": "Ciliated cells were found in the mucosa of the human stomach in three patients. In two cases they occurred in the pyloric mucosa of patients with intestinal metaplasia who were operated on for duodenal ulcer. In the other case, they occurred within a polypoid lesion. They were located in small limited areas. The cells were densely ciliated, each cilium showing a typical 9+2 fibrilar pattern. Ciliated cells are not found in normal gastric mucosa and this suggests that they occur only in pathologically altered mucosa.", "contents": "An electron microscopic study of the ciliated cells in the human gastric mucosa. Ciliated cells were found in the mucosa of the human stomach in three patients. In two cases they occurred in the pyloric mucosa of patients with intestinal metaplasia who were operated on for duodenal ulcer. In the other case, they occurred within a polypoid lesion. They were located in small limited areas. The cells were densely ciliated, each cilium showing a typical 9+2 fibrilar pattern. Ciliated cells are not found in normal gastric mucosa and this suggests that they occur only in pathologically altered mucosa.", "PMID": 973783}
{"id": "pubmed23n0032_9503", "title": "Interosseous muscles in claw finger.", "content": "The third and fourth fingers of the normal hands of 75 adult subjects were tested for active finger extension with the wrists in neutral position. Seventy-two of the subjects could not actively extend the interphalangeal (IP) joints when the metacarpophalangeal (MCP) joints were passively and maximally extended. However, the IP joints could be passively extended without pain in 67 subjects and with pain in 5 subjects. This indicated that the inability to actively extend the IP joints when the MCP joints were maximally extended is seldom due to tightness of the flexor tendons. Electromyographic recordings of the interossei muscles in one subject showed voluntary activity of these muscles with the MCP joints maximally extended while the IP joints remained flexed. A macrospoic study was made of the dissected third and fourth fingers of five embalmed cadaver hands and, in two of these specimens polygraphic recordings were made with the use of the strain gauge. In each of these specimens, when traction was applied in the appropriate interosseous muscle, movement of the lateral band of the extensor aponeurosis was noted when the MCP joint was at neutral or slight flexion, while no motion could be detected when the MCP joint was in maximal extension. Thus, maximal extension of the MCP joint blocks not only the action of the extensor digitorum communis tendon, as shown by Mulder and Landsmeer, but also blocks the action of the interossei in extension of the fingers. This explains the mechanism of development of claw finger in those cases without intrinsic hand muscle paralysis.", "contents": "Interosseous muscles in claw finger. The third and fourth fingers of the normal hands of 75 adult subjects were tested for active finger extension with the wrists in neutral position. Seventy-two of the subjects could not actively extend the interphalangeal (IP) joints when the metacarpophalangeal (MCP) joints were passively and maximally extended. However, the IP joints could be passively extended without pain in 67 subjects and with pain in 5 subjects. This indicated that the inability to actively extend the IP joints when the MCP joints were maximally extended is seldom due to tightness of the flexor tendons. Electromyographic recordings of the interossei muscles in one subject showed voluntary activity of these muscles with the MCP joints maximally extended while the IP joints remained flexed. A macrospoic study was made of the dissected third and fourth fingers of five embalmed cadaver hands and, in two of these specimens polygraphic recordings were made with the use of the strain gauge. In each of these specimens, when traction was applied in the appropriate interosseous muscle, movement of the lateral band of the extensor aponeurosis was noted when the MCP joint was at neutral or slight flexion, while no motion could be detected when the MCP joint was in maximal extension. Thus, maximal extension of the MCP joint blocks not only the action of the extensor digitorum communis tendon, as shown by Mulder and Landsmeer, but also blocks the action of the interossei in extension of the fingers. This explains the mechanism of development of claw finger in those cases without intrinsic hand muscle paralysis.", "PMID": 973784}
{"id": "pubmed23n0032_9504", "title": "Hand dominance and scoliosis in Duchenne muscular dystrophy.", "content": "Twenty-three patients with Duchenne muscular dystrophy and scoliosis were studied. In all but one patient the major convexity was to the side of the dominant hand. The unsupported growing spine is easily unbalanced by asymmetrical forces imposed on it. From our observations we believe major use of a single upper extremity will result in scoliosis with the major convexity toward the side of the dominant hand. Management should include counterbalancing the postural abnormality imposed by hand dominance as well as unloading the spine frequently during the patient's waking hours.", "contents": "Hand dominance and scoliosis in Duchenne muscular dystrophy. Twenty-three patients with Duchenne muscular dystrophy and scoliosis were studied. In all but one patient the major convexity was to the side of the dominant hand. The unsupported growing spine is easily unbalanced by asymmetrical forces imposed on it. From our observations we believe major use of a single upper extremity will result in scoliosis with the major convexity toward the side of the dominant hand. Management should include counterbalancing the postural abnormality imposed by hand dominance as well as unloading the spine frequently during the patient's waking hours.", "PMID": 973785}
{"id": "pubmed23n0032_9505", "title": "Benign hydroarthrosis of knee in patients with spinal cord injury.", "content": "Swelling of the knee joint occurred in 8 of 35 patients with spinal cord injury; in seven of the eight patients the swelling developed within the first eight months after injury. Six of the eight patients had flaccid paraplegia. None of the patients showed any constitutional or local changes other than swelling. No laboratory evidence of inflammation could be detected. Swelling disappeared in two to six weeks and no sequelae were noted. Although trauma appeared to be the most likely cause of the swelling. Only one of four joints showed hemarthrosis on aspiration.", "contents": "Benign hydroarthrosis of knee in patients with spinal cord injury. Swelling of the knee joint occurred in 8 of 35 patients with spinal cord injury; in seven of the eight patients the swelling developed within the first eight months after injury. Six of the eight patients had flaccid paraplegia. None of the patients showed any constitutional or local changes other than swelling. No laboratory evidence of inflammation could be detected. Swelling disappeared in two to six weeks and no sequelae were noted. Although trauma appeared to be the most likely cause of the swelling. Only one of four joints showed hemarthrosis on aspiration.", "PMID": 973786}
{"id": "pubmed23n0032_9506", "title": "Marriage and divorce after spinal cord injury.", "content": "The outcome o,f marriages contracted after spinal cord injury suffered by males was examined via the questionnaire method. Of those whose first marriage occurred after their injury, the divorce rate was 24.4%. Of those whose were married prior to injury and then remarried following injury, 16.4% were divorced. If all post-injury marriages are considered, the divorce rate is 23.1%. This is close to the divorce rate of the United States as a whole. The outcome of post-injury marriages was also examined with respect to time since injury, level of injury, presence of post-injury children, and post-injury education and employment. Particular attention was given to those males who had never been married prior to their injury.", "contents": "Marriage and divorce after spinal cord injury. The outcome o,f marriages contracted after spinal cord injury suffered by males was examined via the questionnaire method. Of those whose first marriage occurred after their injury, the divorce rate was 24.4%. Of those whose were married prior to injury and then remarried following injury, 16.4% were divorced. If all post-injury marriages are considered, the divorce rate is 23.1%. This is close to the divorce rate of the United States as a whole. The outcome of post-injury marriages was also examined with respect to time since injury, level of injury, presence of post-injury children, and post-injury education and employment. Particular attention was given to those males who had never been married prior to their injury.", "PMID": 973787}
{"id": "pubmed23n0032_9507", "title": "Ankle-knee synchronous in a new endoskeletal above-knee prosthetic mechanism: a preliminary report.", "content": "A new above-knee prosthetic mechanism with synchronous motions of the knee and ankle has been designed based on kinematic principles. Knee flexion is synchronized with ankle dorsiflexion by a synchronizer rod and lever. Unlike other above-knee units, the knee joint is aligned about one inch in front of a line between the hip and the ankle joints, which appears to decrease the energy requirements of gait. The swing-phase control system provides constant friction during ordinary cadence and variable friction during rapid swinging of the leg. The addition of a quadrilateral socket, a modified SACH foot and a cosmetic foam plastic cover provides the complete endoskeletal above-knee prosthesis. The resulting gait has been found to be faster, easier and more natural, and the three optional knee settings serve the needs of different amputees and activities.", "contents": "Ankle-knee synchronous in a new endoskeletal above-knee prosthetic mechanism: a preliminary report. A new above-knee prosthetic mechanism with synchronous motions of the knee and ankle has been designed based on kinematic principles. Knee flexion is synchronized with ankle dorsiflexion by a synchronizer rod and lever. Unlike other above-knee units, the knee joint is aligned about one inch in front of a line between the hip and the ankle joints, which appears to decrease the energy requirements of gait. The swing-phase control system provides constant friction during ordinary cadence and variable friction during rapid swinging of the leg. The addition of a quadrilateral socket, a modified SACH foot and a cosmetic foam plastic cover provides the complete endoskeletal above-knee prosthesis. The resulting gait has been found to be faster, easier and more natural, and the three optional knee settings serve the needs of different amputees and activities.", "PMID": 973790}
{"id": "pubmed23n0032_9508", "title": "A mechanical resting surface: its effect on pressure distribution.", "content": "This study demonstrates the potential effectiveness of a dynamic resting surface which, at regular intervals, provides sitting patients complete relief of pressure for short periods of time. Resting pressure extremes ranged from 0 mm Hg to 160 mm Hg, depending on the position of the roller supports. At all positions, including the area beneath the ischial tuberosities, the pressures were well within the range of capillary pressure and usually dropped to zero pressure at regular intervals. This mechanical surface may be the beginning of a new concept in resting surfaces. In addition to providing intermittent relief of pressure to the sitting area, the surface lends itself and the patient to a mobility that has not been heretofore possible.", "contents": "A mechanical resting surface: its effect on pressure distribution. This study demonstrates the potential effectiveness of a dynamic resting surface which, at regular intervals, provides sitting patients complete relief of pressure for short periods of time. Resting pressure extremes ranged from 0 mm Hg to 160 mm Hg, depending on the position of the roller supports. At all positions, including the area beneath the ischial tuberosities, the pressures were well within the range of capillary pressure and usually dropped to zero pressure at regular intervals. This mechanical surface may be the beginning of a new concept in resting surfaces. In addition to providing intermittent relief of pressure to the sitting area, the surface lends itself and the patient to a mobility that has not been heretofore possible.", "PMID": 973791}
{"id": "pubmed23n0032_9509", "title": "Inhibition of cellular DNA synthesis in hamster kidney cells infected with western equine encephalitis virus.", "content": "Infection of BHK cells with western equine encephalitis (WEE) virus resulted in rapid inhibition of cellular DNA synthesis. The rate of inhibition of DNA synthesis depended on the multiplicity of infection, and was closely related to virus replication. Cellular DNA synthesis was not inhibited in infected BHK cells that had been irradiated with ultraviolet radiation. These results indicated that a functional viral genome was required for the inhibition of DNA synthesis by WEE virus. The sharp decrease in thymidine incorporation into the acid-insoluble fraction was not due to a change in the intracellular pool of the acid-soluble fraction. Sedimentation analysis in alkaline sucrose gradients was used to show that cellular DNA was not degraded during WEE viirus infection. DNA polymerase activity in infected cells was not significantly reduced.", "contents": "Inhibition of cellular DNA synthesis in hamster kidney cells infected with western equine encephalitis virus. Infection of BHK cells with western equine encephalitis (WEE) virus resulted in rapid inhibition of cellular DNA synthesis. The rate of inhibition of DNA synthesis depended on the multiplicity of infection, and was closely related to virus replication. Cellular DNA synthesis was not inhibited in infected BHK cells that had been irradiated with ultraviolet radiation. These results indicated that a functional viral genome was required for the inhibition of DNA synthesis by WEE virus. The sharp decrease in thymidine incorporation into the acid-insoluble fraction was not due to a change in the intracellular pool of the acid-soluble fraction. Sedimentation analysis in alkaline sucrose gradients was used to show that cellular DNA was not degraded during WEE viirus infection. DNA polymerase activity in infected cells was not significantly reduced.", "PMID": 973797}
{"id": "pubmed23n0032_9510", "title": "Characterization of Orungo virus, an orbivirus from Uganda and Nigeria.", "content": "Orungo virus was studied in cell culture and mice by light and electron microscopy. The virus developed in the cytoplasm of infected cells in mouse brain and cell culture in association with a specific viral granular matrix and accompanying filaments. Virus particle size was 63 nm with a core diameter of 34 nm. Most particles were released from infected cells by lysis, but some budded through membranes and assumed a \"pseudoenvelope\". In its morphology and mode of morphogenesis, Orungo virus was indistinguishable from other described orbiviruses.", "contents": "Characterization of Orungo virus, an orbivirus from Uganda and Nigeria. Orungo virus was studied in cell culture and mice by light and electron microscopy. The virus developed in the cytoplasm of infected cells in mouse brain and cell culture in association with a specific viral granular matrix and accompanying filaments. Virus particle size was 63 nm with a core diameter of 34 nm. Most particles were released from infected cells by lysis, but some budded through membranes and assumed a \"pseudoenvelope\". In its morphology and mode of morphogenesis, Orungo virus was indistinguishable from other described orbiviruses.", "PMID": 973798}
{"id": "pubmed23n0032_9511", "title": "Kinetics of heat inactivation of Venezuelan equine encephalomyelitis virus.", "content": "Thermal inactivation of Venezuelan Equine Encephalomyelitis Virus (VEEV) was studied at temperatures from 26 degrees to 55 degrees C. Inactivation of infectivity took place by two thermodynamically different reactions, one of which predominated at temperatures below 44 degrees C and the other at higher temperatures. The presence of 1 or 2 M NaCl stabilized the VEE virus at low temperatures but enhanced the inactivation at high temperatures. This latter effect at temperatures higher than 50 degrees C, is associated with the occurrence of two-component survival curves. The different effects of hypertonic NaCl concentrations at the two ranges of temperature, are related to different mechanisms of inactivation operating at each range (protein denaturation and nucleic acid-RNA breakdown). Different kinetics of thermal inactivation at 55 degrees C were observed between virus strains with different virulence. However, no significant correlations was found between the virulence of the eleven VEE virus strains studied and their thermostability at 37 degres and 55 degrees C.", "contents": "Kinetics of heat inactivation of Venezuelan equine encephalomyelitis virus. Thermal inactivation of Venezuelan Equine Encephalomyelitis Virus (VEEV) was studied at temperatures from 26 degrees to 55 degrees C. Inactivation of infectivity took place by two thermodynamically different reactions, one of which predominated at temperatures below 44 degrees C and the other at higher temperatures. The presence of 1 or 2 M NaCl stabilized the VEE virus at low temperatures but enhanced the inactivation at high temperatures. This latter effect at temperatures higher than 50 degrees C, is associated with the occurrence of two-component survival curves. The different effects of hypertonic NaCl concentrations at the two ranges of temperature, are related to different mechanisms of inactivation operating at each range (protein denaturation and nucleic acid-RNA breakdown). Different kinetics of thermal inactivation at 55 degrees C were observed between virus strains with different virulence. However, no significant correlations was found between the virulence of the eleven VEE virus strains studied and their thermostability at 37 degres and 55 degrees C.", "PMID": 973799}
{"id": "pubmed23n0032_9512", "title": "Demonstration of non-infectious hemagglutinating particles of rabies virus and isolation of the hemagglutinin by disruption of the virion with Nonidet P-40.", "content": "Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutinin (HAnin) was obtained by solubilization of partially purified virions with Nonidet P-40 (NP-40) followed by successive high speed and CsCl density gradient centrifugations. The density of the isolated HAnin averaged 1.28 g/cm3. SDS-polyacrylamide gel electrophoresis of the HAnin demonstrated that it was mainly composed of a glycoprotein (G) with a molecular weight of 83,000. Electron microscopically, it differed from the above non-infectious hemagglutinating particles, being much smaller in size and showing a star- or rosette-like appearance with a diameter of about 25 nm, composed of a central particle surrounded by particles resembling envelope-spikes. Virus-neutralizing (VN) and hemagglutination inhibiting (HI) antibodies were produced in rabbits immunized with the HAnin isolated from virions.", "contents": "Demonstration of non-infectious hemagglutinating particles of rabies virus and isolation of the hemagglutinin by disruption of the virion with Nonidet P-40. Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutinin (HAnin) was obtained by solubilization of partially purified virions with Nonidet P-40 (NP-40) followed by successive high speed and CsCl density gradient centrifugations. The density of the isolated HAnin averaged 1.28 g/cm3. SDS-polyacrylamide gel electrophoresis of the HAnin demonstrated that it was mainly composed of a glycoprotein (G) with a molecular weight of 83,000. Electron microscopically, it differed from the above non-infectious hemagglutinating particles, being much smaller in size and showing a star- or rosette-like appearance with a diameter of about 25 nm, composed of a central particle surrounded by particles resembling envelope-spikes. Virus-neutralizing (VN) and hemagglutination inhibiting (HI) antibodies were produced in rabbits immunized with the HAnin isolated from virions.", "PMID": 973800}
{"id": "pubmed23n0032_9513", "title": "Disease markers in acute multiple sclerosis.", "content": "Three serum components that are known to become elevated during inflammation and tissue destruction, C-reactive protein, C3 proactivator, and orosomucoid, which are acute phase reactants, and serum lgM were measured in patients with multiple sclerosis (MS) hospitalized with acute exacerbations. Significantly elevated levels of one or more of these serum components were found in 12 of 13 patients with clinically active MS. Serial studies in two patients revealed that clinical improvement was accompanied by a decline in the serum levels of these factors. These findings suggest that measurement of these serum proteins may be of value in assessing progress of disease activity in MS patients.", "contents": "Disease markers in acute multiple sclerosis. Three serum components that are known to become elevated during inflammation and tissue destruction, C-reactive protein, C3 proactivator, and orosomucoid, which are acute phase reactants, and serum lgM were measured in patients with multiple sclerosis (MS) hospitalized with acute exacerbations. Significantly elevated levels of one or more of these serum components were found in 12 of 13 patients with clinically active MS. Serial studies in two patients revealed that clinical improvement was accompanied by a decline in the serum levels of these factors. These findings suggest that measurement of these serum proteins may be of value in assessing progress of disease activity in MS patients.", "PMID": 973802}
{"id": "pubmed23n0032_9514", "title": "Perioptic meningiomas. Syndrome of long-standing visual loss, pale disk edema, and optociliary veins.", "content": "Three patients had a syndrome of progressive or long-standing visual loss, pale disk edema, and optociliary veins, indicating that a spheno-orbital meningioma had invaded the anterior perioptic meninges. To diagnose this syndrome, one must distinguish \"disk edema\" from \"papilledema\"; although both result from increased pressure within the perioptic subarachnoid space, disk edema is caused by a variety of lesions just behind the globe, whereas papilledema is caused by remote lesions that raise intracranial pressure. Optociliary veins reflect the slowness of progression of both processes. This ophthalmoscopic diagnosis carries important implications, namely, that the tumor grows very slowly and that surgical treatment will not improve vision. Therefore, excision may not be the treatment of choice.", "contents": "Perioptic meningiomas. Syndrome of long-standing visual loss, pale disk edema, and optociliary veins. Three patients had a syndrome of progressive or long-standing visual loss, pale disk edema, and optociliary veins, indicating that a spheno-orbital meningioma had invaded the anterior perioptic meninges. To diagnose this syndrome, one must distinguish \"disk edema\" from \"papilledema\"; although both result from increased pressure within the perioptic subarachnoid space, disk edema is caused by a variety of lesions just behind the globe, whereas papilledema is caused by remote lesions that raise intracranial pressure. Optociliary veins reflect the slowness of progression of both processes. This ophthalmoscopic diagnosis carries important implications, namely, that the tumor grows very slowly and that surgical treatment will not improve vision. Therefore, excision may not be the treatment of choice.", "PMID": 973803}
{"id": "pubmed23n0032_9515", "title": "Progressive spastic paraparesis and adrenal insufficiency.", "content": "A 10-year-old boy with progressive paraparesis, personality change, and seizures had laboratory evidence of adrenal insufficiency. Pathologic study showed cerebral edema, but no loss of myelin. Notable pathologic changes were limited to the spinal cord, where the corticospinal and spinocerebellar tracts were demyelinated. Lipid analysis of the brain was normal apart from the finding that galactocerebroside contained a higher proportion than normal of alpha-hydroxy fatty acids. We suggest that this case represents a distinct disease, differing importantly from adrenoleukodystrophy. The underlying defect appears to be in the early enzymatic pathway before cholesterol synthesis, although it is also possible that the defect is at the cell membrane.", "contents": "Progressive spastic paraparesis and adrenal insufficiency. A 10-year-old boy with progressive paraparesis, personality change, and seizures had laboratory evidence of adrenal insufficiency. Pathologic study showed cerebral edema, but no loss of myelin. Notable pathologic changes were limited to the spinal cord, where the corticospinal and spinocerebellar tracts were demyelinated. Lipid analysis of the brain was normal apart from the finding that galactocerebroside contained a higher proportion than normal of alpha-hydroxy fatty acids. We suggest that this case represents a distinct disease, differing importantly from adrenoleukodystrophy. The underlying defect appears to be in the early enzymatic pathway before cholesterol synthesis, although it is also possible that the defect is at the cell membrane.", "PMID": 973804}
{"id": "pubmed23n0032_9516", "title": "Acute renal failure. A complication of cerebral angiography.", "content": "Acute renal failure after cerebral angiography is, to our knowledge, previously undescribed, yet well documented after other forms of angiography and intravenous urography. We will describe four patients with this complicaton and discuss factors in its pathogenesis.", "contents": "Acute renal failure. A complication of cerebral angiography. Acute renal failure after cerebral angiography is, to our knowledge, previously undescribed, yet well documented after other forms of angiography and intravenous urography. We will describe four patients with this complicaton and discuss factors in its pathogenesis.", "PMID": 973805}
{"id": "pubmed23n0032_9517", "title": "Somatic anxiety attacks and propranolol.", "content": "Patients with acute anxiety-like attacks are often evaluated for various medical conditions, and, when nothing is found, their symptoms are attributed to psychogenic causes. \"beta-Adrenergic hyperactivity\" should be considered in these patients. Six patients are described who, we believe, are suffering from this condition. Low rates of isoproterenol hydrochloride infusion-precipitated symptoms that mimicked their spontaneous somatic anxiety attacks. Treatment with propranolol hydrochloride produced longterm symptomatic relief.", "contents": "Somatic anxiety attacks and propranolol. Patients with acute anxiety-like attacks are often evaluated for various medical conditions, and, when nothing is found, their symptoms are attributed to psychogenic causes. \"beta-Adrenergic hyperactivity\" should be considered in these patients. Six patients are described who, we believe, are suffering from this condition. Low rates of isoproterenol hydrochloride infusion-precipitated symptoms that mimicked their spontaneous somatic anxiety attacks. Treatment with propranolol hydrochloride produced longterm symptomatic relief.", "PMID": 973806}
{"id": "pubmed23n0032_9518", "title": "Hypothenar Dimpling. A peripheral equivalent of Hemifacial Spasm?", "content": "In two patients, the skin over both hypothenar eminences underwent intermittent, spontaneous, irregular, dimpling contractions. The dimpling was benign, and was the result of spontaneous discharge of motor units in the palmaris brevis muscle. Electrophysiological investigations localized the site of origin of the discharge to the ulnar nerve, possibly at the wrist, but there was no clinical or physiological evidence of neuropathy or of nerve compression. In many respects, the clinical and electrophysiological features of hypothenar dimpling resemble hemifacial spasm.", "contents": "Hypothenar Dimpling. A peripheral equivalent of Hemifacial Spasm? In two patients, the skin over both hypothenar eminences underwent intermittent, spontaneous, irregular, dimpling contractions. The dimpling was benign, and was the result of spontaneous discharge of motor units in the palmaris brevis muscle. Electrophysiological investigations localized the site of origin of the discharge to the ulnar nerve, possibly at the wrist, but there was no clinical or physiological evidence of neuropathy or of nerve compression. In many respects, the clinical and electrophysiological features of hypothenar dimpling resemble hemifacial spasm.", "PMID": 973807}
{"id": "pubmed23n0032_9519", "title": "Cell volume and permeability of oxygen-and glucose-deprived retina in vitro.", "content": "Rabbit retina was deprived of O(2) and glucose in vitro for up to four hours at 37 C. Intracellular volume was measured, using inulin as an extracellular marker. After a 30-minute latency, cells swelled rapidly to more than twice normal volume while extracellular volume was unchanged. Intracellular accumulation of water was not reversed by resupply of oxygen and glucose. Permeability to small molecules was assessed with mannitol. The ratio of mannitol space to inulin space averaged 1.0 in controls. This ratio remained 1.0 up to 30 minutes of deprivation, but increased to 1.2 by 60 minutes. Permeability to large molecules was assessed from the rate of loss of isotopically labeled cell protein into the medium. There was no difference between control and deprived retinas up to three hours.", "contents": "Cell volume and permeability of oxygen-and glucose-deprived retina in vitro. Rabbit retina was deprived of O(2) and glucose in vitro for up to four hours at 37 C. Intracellular volume was measured, using inulin as an extracellular marker. After a 30-minute latency, cells swelled rapidly to more than twice normal volume while extracellular volume was unchanged. Intracellular accumulation of water was not reversed by resupply of oxygen and glucose. Permeability to small molecules was assessed with mannitol. The ratio of mannitol space to inulin space averaged 1.0 in controls. This ratio remained 1.0 up to 30 minutes of deprivation, but increased to 1.2 by 60 minutes. Permeability to large molecules was assessed from the rate of loss of isotopically labeled cell protein into the medium. There was no difference between control and deprived retinas up to three hours.", "PMID": 973808}
{"id": "pubmed23n0032_9520", "title": "Retina subjected to components of ischemia in vitro. Selective vulnerability and minimum lethal exposure of neurons and glia to oxygen and/or glucose deprivation and to loss of exchange with incubating medium.", "content": "Rabbit retinas were incubated at 37 C in media lacking oxygen, glucose, or both, or sealed in a small compartment without medium to convert them to a \"closed system.\" They were then returned to control medium before being fixed for microscopy. Other retinas were incubated only in control medium and then fixed. Conversion of the retina to a closed system caused irreversible damage to all cell types within 40 minutes. Combined deprivation of oxygen and glucose also irreversibly damaged the neuronal cells within 40 minutes, but Mueller cells,the principal glial cells of the retina, were not irreversibly altered by 90 minutes of the deprivation. Deprivation of oxygen alone caused irreversible damage to receptor cells in 80 minutes, but the cells of the inner nuclear layer, ganglion cells, and Mueller cells retained normal structure for at least 180 minutes. Deprivation of glucose alone damaged receptor cells in 160 minutes and the other neuronal cells in 180 minutes, but did not irreversibly damage Mueller cells by 200 minutes.", "contents": "Retina subjected to components of ischemia in vitro. Selective vulnerability and minimum lethal exposure of neurons and glia to oxygen and/or glucose deprivation and to loss of exchange with incubating medium. Rabbit retinas were incubated at 37 C in media lacking oxygen, glucose, or both, or sealed in a small compartment without medium to convert them to a \"closed system.\" They were then returned to control medium before being fixed for microscopy. Other retinas were incubated only in control medium and then fixed. Conversion of the retina to a closed system caused irreversible damage to all cell types within 40 minutes. Combined deprivation of oxygen and glucose also irreversibly damaged the neuronal cells within 40 minutes, but Mueller cells,the principal glial cells of the retina, were not irreversibly altered by 90 minutes of the deprivation. Deprivation of oxygen alone caused irreversible damage to receptor cells in 80 minutes, but the cells of the inner nuclear layer, ganglion cells, and Mueller cells retained normal structure for at least 180 minutes. Deprivation of glucose alone damaged receptor cells in 160 minutes and the other neuronal cells in 180 minutes, but did not irreversibly damage Mueller cells by 200 minutes.", "PMID": 973809}
{"id": "pubmed23n0032_9521", "title": "Progressive rubella panencephalitis.", "content": "A patient with progressive rubella panencephalitis developed initial symptoms of neurologic deterioration 12 years after childhood German measles. Progressive rubella panencephalitis should be considered in adolescents with progressive dementia attended by pyradmidal and cerebellar dysfunction.", "contents": "Progressive rubella panencephalitis. A patient with progressive rubella panencephalitis developed initial symptoms of neurologic deterioration 12 years after childhood German measles. Progressive rubella panencephalitis should be considered in adolescents with progressive dementia attended by pyradmidal and cerebellar dysfunction.", "PMID": 973810}
{"id": "pubmed23n0032_9522", "title": "Brain stem auditory-evoked responses in suspected central pontine myelinolysis.", "content": "Central pontine myelinolysis was suspected in two chronic alcoholics who developed and recovered from a progressive spastic paresis of all muscles, that derived innervation at and below the level of the pons. In both cases, short-latency auditory-evoked responses aided in the diagnosis by indicating a slowing of conduction in the pontine auditory pathway, which varied in degree with the severity of the clinical manifestations of pontine demyelination.", "contents": "Brain stem auditory-evoked responses in suspected central pontine myelinolysis. Central pontine myelinolysis was suspected in two chronic alcoholics who developed and recovered from a progressive spastic paresis of all muscles, that derived innervation at and below the level of the pons. In both cases, short-latency auditory-evoked responses aided in the diagnosis by indicating a slowing of conduction in the pontine auditory pathway, which varied in degree with the severity of the clinical manifestations of pontine demyelination.", "PMID": 973811}
{"id": "pubmed23n0032_9523", "title": "Perforating diathermy coagulation for retinal angiomas.", "content": "Perforating diathermy coagulation can be used efficiently to destroy large angiomas of the retina. Five patients who had developed large angiomas (greater than 5 disc diameters) in association with various retinal diseases were treated with this technique. The follow-up period ranged from 1 1/2 to 7 years, except for one case in which the patient died five days after the surgical procedure.  Several techniques of perforating diathermy are described, and the indications, surgical precautions, and complications of each method are discussed.", "contents": "Perforating diathermy coagulation for retinal angiomas. Perforating diathermy coagulation can be used efficiently to destroy large angiomas of the retina. Five patients who had developed large angiomas (greater than 5 disc diameters) in association with various retinal diseases were treated with this technique. The follow-up period ranged from 1 1/2 to 7 years, except for one case in which the patient died five days after the surgical procedure.  Several techniques of perforating diathermy are described, and the indications, surgical precautions, and complications of each method are discussed.", "PMID": 973818}
{"id": "pubmed23n0032_9524", "title": "Visual effects of pilocarpine in glaucoma comparative study of administration by eyedrops or by ocular therapeutic systems.", "content": "Eighteen glaucoma patients each received four pilocarpine regimens in random sequence: 1% and 4% eyedrops and 20mug/hr and 40mug/hr ocular therapeutic systems. Unimportant changes in refraction, near vision, and distance vision occurred during the use of either ocular therapeutic system. Miosis with ocular therapeutic systems was almost always less intense and variable than with eyedrops. Refractive changes occurred in 12 patients following 1% pilocarpine and in 16 patients following 4% pilocarpine drops; decreased distance vision occurred in nine patients after 1% drops and in 12 patients after 4% drops. Fewer patients showed decreases in near vision. In all cases, visual effects peaked one half hour after eyedrop instillations and returned gradually toward normal in the next two to three hours. Intraocular pressure levels were within comparable ranges during all four treatments, but the ocular therapeutic systems maintained pressure at a more constant level.", "contents": "Visual effects of pilocarpine in glaucoma comparative study of administration by eyedrops or by ocular therapeutic systems. Eighteen glaucoma patients each received four pilocarpine regimens in random sequence: 1% and 4% eyedrops and 20mug/hr and 40mug/hr ocular therapeutic systems. Unimportant changes in refraction, near vision, and distance vision occurred during the use of either ocular therapeutic system. Miosis with ocular therapeutic systems was almost always less intense and variable than with eyedrops. Refractive changes occurred in 12 patients following 1% pilocarpine and in 16 patients following 4% pilocarpine drops; decreased distance vision occurred in nine patients after 1% drops and in 12 patients after 4% drops. Fewer patients showed decreases in near vision. In all cases, visual effects peaked one half hour after eyedrop instillations and returned gradually toward normal in the next two to three hours. Intraocular pressure levels were within comparable ranges during all four treatments, but the ocular therapeutic systems maintained pressure at a more constant level.", "PMID": 973819}
{"id": "pubmed23n0032_9525", "title": "Autosomal recessive retinitis pigmentosa and Coats disease: a presumed familial incidence.", "content": "Three of four siblings of normal parents had a combination of Coats disease and retinitis pigmentosa bilaterally. Our report of their respective cases is, to our knowledge, the first report of a familial incidence of the combination of the two diseases in an individual and supports the implication that Coats disease may be a genetic abnormality. The pathological process was essentially the same in all three patients, although it occurred differently in one of the three siblings and gave rise to an altered functional and clinical picture. The similarities may indicate that the convenient categories of \"tapeto-retinal degenerations\" are clinical variations of the same genetic abnormality.", "contents": "Autosomal recessive retinitis pigmentosa and Coats disease: a presumed familial incidence. Three of four siblings of normal parents had a combination of Coats disease and retinitis pigmentosa bilaterally. Our report of their respective cases is, to our knowledge, the first report of a familial incidence of the combination of the two diseases in an individual and supports the implication that Coats disease may be a genetic abnormality. The pathological process was essentially the same in all three patients, although it occurred differently in one of the three siblings and gave rise to an altered functional and clinical picture. The similarities may indicate that the convenient categories of \"tapeto-retinal degenerations\" are clinical variations of the same genetic abnormality.", "PMID": 973820}
{"id": "pubmed23n0032_9526", "title": "The conjunctiva in senile lens exfoliation.", "content": "Conjunctival biopsy specimens from both eyes of a series of seven patients with evidence of unilateral senile exfoliation of the lens were examined by electron microscopy. Exfoliation fibers were found in both eyes of each patient. This evidence suggests that the conjunctiva is an independent source of exfoliation that precedes the development of exfoliation in the lens.", "contents": "The conjunctiva in senile lens exfoliation. Conjunctival biopsy specimens from both eyes of a series of seven patients with evidence of unilateral senile exfoliation of the lens were examined by electron microscopy. Exfoliation fibers were found in both eyes of each patient. This evidence suggests that the conjunctiva is an independent source of exfoliation that precedes the development of exfoliation in the lens.", "PMID": 973821}
{"id": "pubmed23n0032_9527", "title": "Clinicopathologic correlations in diabetic retinopathy. I. Histology and fluorescein angiography of microaneurysms.", "content": "A patient with adult-onset diabetes mellitus was referred with a diagnosis of malignant melanoma of the choroid of the left eye. A nonproliferative type of diabetic retinopathy was present, which was studied and documented by stereoscopic fundus photographs and fluorescein angiograms. Following enucleation, the microangiopathies were correlated histologically, using the retinal trypsin digest technique. Four types of microaneurysms were seen histologically that were believed to represent stages in the development of this lesion. Most thin-walled aneurysms tightly packed with erythrocytes did not fluoresce. Aneurysms that were hypercellular and those with thick walls showed early and late fluorescence. Intraretinal microvascular abnormalities were hypercellular dilated channels. Those that take origin from terminal arterioles are believed to represent attempts at neovascularization.", "contents": "Clinicopathologic correlations in diabetic retinopathy. I. Histology and fluorescein angiography of microaneurysms. A patient with adult-onset diabetes mellitus was referred with a diagnosis of malignant melanoma of the choroid of the left eye. A nonproliferative type of diabetic retinopathy was present, which was studied and documented by stereoscopic fundus photographs and fluorescein angiograms. Following enucleation, the microangiopathies were correlated histologically, using the retinal trypsin digest technique. Four types of microaneurysms were seen histologically that were believed to represent stages in the development of this lesion. Most thin-walled aneurysms tightly packed with erythrocytes did not fluoresce. Aneurysms that were hypercellular and those with thick walls showed early and late fluorescence. Intraretinal microvascular abnormalities were hypercellular dilated channels. Those that take origin from terminal arterioles are believed to represent attempts at neovascularization.", "PMID": 973822}
{"id": "pubmed23n0032_9528", "title": "Phakomatous choristoma of eyelid: electron microscopical confirmation of lenticular derivation.", "content": "This article presents the sixth recorded example of phakomatous choristoma of the eyelid. The tumor occurred in a 4-month-old boy and was located in the lower lid near the inner canthus. The findings from light microscopy included epithelial, tubular structures with associated patches of thick, irregular basement membrane. The results of electron microscopy (ie, epithelial cells with a scarcity of organelles, homogeneous type of basement membrane, cytoplasmic microfilaments, segments of fascia occludens, and degenerated cells with peculiar interdigitations of their plasmalemmas) are all consistent with lenticular derivation, as originally proposed by Zimmerman.", "contents": "Phakomatous choristoma of eyelid: electron microscopical confirmation of lenticular derivation. This article presents the sixth recorded example of phakomatous choristoma of the eyelid. The tumor occurred in a 4-month-old boy and was located in the lower lid near the inner canthus. The findings from light microscopy included epithelial, tubular structures with associated patches of thick, irregular basement membrane. The results of electron microscopy (ie, epithelial cells with a scarcity of organelles, homogeneous type of basement membrane, cytoplasmic microfilaments, segments of fascia occludens, and degenerated cells with peculiar interdigitations of their plasmalemmas) are all consistent with lenticular derivation, as originally proposed by Zimmerman.", "PMID": 973823}
{"id": "pubmed23n0032_9529", "title": "Human corneal endothelial layer repair during organ culture.", "content": "Circular freeze-thaw endothelial wounds were created on paired human corneas. Ultrastructural and physiological studies were performed after organ culture (OC) incubation at 37 C from 1 to 21 days as well as on fresh noncultured controls. As early as 24 hours after injury, OC corneas demonstrated ultrastructurally intact endothelial cells at the margin of the wound, elongating and sliding toward its center. All OC corneas were completely covered by ultrastructurally intact and physiologically functioning endothelial cells by seven days of OC. These cells were approximately twice normal size. Enlarged endothelial cells that maintained deturgescence function were seen in the wounded area after 14 and 21 days of OC. None of the fresh controls demonstrated deturgescence function and in none could ultrastructurally intact endothelial cells be found in the area of the wound. This confirms our hypothesis that during 37 C OC incubation, human corneal endothelium repairs defects in its layer by cells that are physiologically and ultrastructurally intact.", "contents": "Human corneal endothelial layer repair during organ culture. Circular freeze-thaw endothelial wounds were created on paired human corneas. Ultrastructural and physiological studies were performed after organ culture (OC) incubation at 37 C from 1 to 21 days as well as on fresh noncultured controls. As early as 24 hours after injury, OC corneas demonstrated ultrastructurally intact endothelial cells at the margin of the wound, elongating and sliding toward its center. All OC corneas were completely covered by ultrastructurally intact and physiologically functioning endothelial cells by seven days of OC. These cells were approximately twice normal size. Enlarged endothelial cells that maintained deturgescence function were seen in the wounded area after 14 and 21 days of OC. None of the fresh controls demonstrated deturgescence function and in none could ultrastructurally intact endothelial cells be found in the area of the wound. This confirms our hypothesis that during 37 C OC incubation, human corneal endothelium repairs defects in its layer by cells that are physiologically and ultrastructurally intact.", "PMID": 973824}
{"id": "pubmed23n0032_9530", "title": "Examination and Photography of donor corneal endothelium.", "content": "In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.", "contents": "Examination and Photography of donor corneal endothelium. In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.", "PMID": 973825}
{"id": "pubmed23n0032_9531", "title": "Mental illness in the biological and adoptive families of adopted individuals who have become schizophrenic.", "content": "In a sample of 5483 adults who had been legally adopted early in life by persons not biologically related to them, 33 were identified, from mental hospital records for whom a diagnosis of definite schizophrenia (chronic, latent, or acute) could be agreed upon by four raters. An equal number of matched controls were selected from the sample of adopted individuals who had never been admitted to a mental hospital. Ninety percent of the living parents, siblings, and half-siblings, biological and adoptive, cooperated in an extensive psychiatric interview permitting a consensus diagnosis by three blind raters. Schizophrenia and uncertain schizophrenia were found to be significantly concentrated in the population genetically related to the schizophrenic adoptees. Their adoptive relatives did not differ from the control populations in the prevalence of schizophrenic illness.", "contents": "Mental illness in the biological and adoptive families of adopted individuals who have become schizophrenic. In a sample of 5483 adults who had been legally adopted early in life by persons not biologically related to them, 33 were identified, from mental hospital records for whom a diagnosis of definite schizophrenia (chronic, latent, or acute) could be agreed upon by four raters. An equal number of matched controls were selected from the sample of adopted individuals who had never been admitted to a mental hospital. Ninety percent of the living parents, siblings, and half-siblings, biological and adoptive, cooperated in an extensive psychiatric interview permitting a consensus diagnosis by three blind raters. Schizophrenia and uncertain schizophrenia were found to be significantly concentrated in the population genetically related to the schizophrenic adoptees. Their adoptive relatives did not differ from the control populations in the prevalence of schizophrenic illness.", "PMID": 973827}
{"id": "pubmed23n0032_9532", "title": "Discussion of genetics and mental health.", "content": "The preceding articles by Kety, Gershon et al., and Omenn are discussed. Ongoing research on children of one or two schizophrenic parents is described as representing the opportunity to study precipitating environmental factors.", "contents": "Discussion of genetics and mental health. The preceding articles by Kety, Gershon et al., and Omenn are discussed. Ongoing research on children of one or two schizophrenic parents is described as representing the opportunity to study precipitating environmental factors.", "PMID": 973828}
{"id": "pubmed23n0032_9533", "title": "Genetic analysis of behaviors related to the solution of a detour learning task.", "content": "A diallel cross involving five noninbred (purebred) lines of Japanese quail indicated that additive and nonadditive genetic effects had important influences on behaviors involved in a detour learning task. Social isolation for 3 hr was used to provide additional motivation which enabled detection of differences among strains. The percentage of quail, within purebred lines, that solved a 4-min detour task three or more consecutive times ranged from 52 to 72. Differences among purebreds were significant for most of the components of the learning task. Further, the data suggested strain differences associated with the successful attainment of the desired goal when there was a similar level of general activity.", "contents": "Genetic analysis of behaviors related to the solution of a detour learning task. A diallel cross involving five noninbred (purebred) lines of Japanese quail indicated that additive and nonadditive genetic effects had important influences on behaviors involved in a detour learning task. Social isolation for 3 hr was used to provide additional motivation which enabled detection of differences among strains. The percentage of quail, within purebred lines, that solved a 4-min detour task three or more consecutive times ranged from 52 to 72. Differences among purebreds were significant for most of the components of the learning task. Further, the data suggested strain differences associated with the successful attainment of the desired goal when there was a similar level of general activity.", "PMID": 973829}
{"id": "pubmed23n0032_9534", "title": "Homeostatic drive counteracting selection for positive and negative phototaxis and geotaxis in Drosophila pseudoobscura.", "content": "Tested in Hirsch-Hadler mazes, Drosophila pseudoobscura is on the average geoneutral and photoneutral. Strongly geo- and photopositive and geo- and photonegative populations can be obtained by artificial selection, but upon relaxation of the selection they tend to relapse toward neutrality. This genetic homeostasis is due to natural selection favoring neutrality. Experiments are described in which artificial selection for positivity and for negativity was deliberately made so weak that it only counterbalanced the nautral selection or \"homeostatic drive\" and the effects of cross-migration. Under these conditions, the behavior of the population artificially selected for positivity diverges only slightly from that of the population selected for negativity, but, at least in females, both populations move closer to neutrality.", "contents": "Homeostatic drive counteracting selection for positive and negative phototaxis and geotaxis in Drosophila pseudoobscura. Tested in Hirsch-Hadler mazes, Drosophila pseudoobscura is on the average geoneutral and photoneutral. Strongly geo- and photopositive and geo- and photonegative populations can be obtained by artificial selection, but upon relaxation of the selection they tend to relapse toward neutrality. This genetic homeostasis is due to natural selection favoring neutrality. Experiments are described in which artificial selection for positivity and for negativity was deliberately made so weak that it only counterbalanced the nautral selection or \"homeostatic drive\" and the effects of cross-migration. Under these conditions, the behavior of the population artificially selected for positivity diverges only slightly from that of the population selected for negativity, but, at least in females, both populations move closer to neutrality.", "PMID": 973830}
{"id": "pubmed23n0032_9535", "title": "The Maudsley reactive and nonreactive strains of rats: a clarification.", "content": "The origin of the Maudsley reactive and nonreactive strains of rats and the construction of the survey of researches using them are briefly reviewed in the light of Archer's criticisms. The reasons for reasserting that they represent a valid dichotomy in emotionality are summarized.", "contents": "The Maudsley reactive and nonreactive strains of rats: a clarification. The origin of the Maudsley reactive and nonreactive strains of rats and the construction of the survey of researches using them are briefly reviewed in the light of Archer's criticisms. The reasons for reasserting that they represent a valid dichotomy in emotionality are summarized.", "PMID": 973831}
{"id": "pubmed23n0032_9536", "title": "[Association of primary hypothyroidism with preconcious puberty. Presentation of clinical case and the effect of acute inhibition of thyrotropin on the gonadal activity].", "content": "An 8 2/12 year-old girl with acquired primary hypothyroidism and precociuos puberty is described. Thyroid hormone treatment resulted in the regression of the sexual development, but moderate asymptomatic papilledema appeared as a result of therapy. Serial vaginal smears and measurements of urinary estrogen excretion during acute thyrotropin (TSH) inhibition with 75 mug/day of triiodthyronine (T3), showed a rapid drop in ovaric estrogen production up to prepubertal stage at 17 days of T3 administration. Moreover, a group of children with untreated hypothyroidism was followed through the first 3 to 12 months of thyroid therapy and none developed signs of increased intracranial pressure. These findings as well as the possible pathophysiology involved in this association are discussed.", "contents": "[Association of primary hypothyroidism with preconcious puberty. Presentation of clinical case and the effect of acute inhibition of thyrotropin on the gonadal activity]. An 8 2/12 year-old girl with acquired primary hypothyroidism and precociuos puberty is described. Thyroid hormone treatment resulted in the regression of the sexual development, but moderate asymptomatic papilledema appeared as a result of therapy. Serial vaginal smears and measurements of urinary estrogen excretion during acute thyrotropin (TSH) inhibition with 75 mug/day of triiodthyronine (T3), showed a rapid drop in ovaric estrogen production up to prepubertal stage at 17 days of T3 administration. Moreover, a group of children with untreated hypothyroidism was followed through the first 3 to 12 months of thyroid therapy and none developed signs of increased intracranial pressure. These findings as well as the possible pathophysiology involved in this association are discussed.", "PMID": 973854}
{"id": "pubmed23n0032_9537", "title": "[Relationships between thyrotropin, growth hormone and insulin in hypothyroidism].", "content": "Since growth hormone has been found to be inhibited in many cases of untreated primary hypothyroidism, we tried to investigate whether the high thyrotropin levels or the lack of thyroid hormones is the responsible factor. Six primary hypothyrotic patients were kept euthyroid by means of high and constant doses of thyroid extract. Growth hormone immunoassays were performed before and after three days of thyrotropin stimulation. Immunoassayable insulin was also investigated under the same experimental conditions. Although a moderate inhibition of growth hormone after thyrotopin stimulation was observed, the magnitude of this inhibition was not as marked as it has bee described in untreated primary hypothyrotics. There was no difference in insulin production before and after thyrotropin administration.", "contents": "[Relationships between thyrotropin, growth hormone and insulin in hypothyroidism]. Since growth hormone has been found to be inhibited in many cases of untreated primary hypothyroidism, we tried to investigate whether the high thyrotropin levels or the lack of thyroid hormones is the responsible factor. Six primary hypothyrotic patients were kept euthyroid by means of high and constant doses of thyroid extract. Growth hormone immunoassays were performed before and after three days of thyrotropin stimulation. Immunoassayable insulin was also investigated under the same experimental conditions. Although a moderate inhibition of growth hormone after thyrotopin stimulation was observed, the magnitude of this inhibition was not as marked as it has bee described in untreated primary hypothyrotics. There was no difference in insulin production before and after thyrotropin administration.", "PMID": 973855}
{"id": "pubmed23n0032_9538", "title": "[Integration of education and services as a base for the formation of human resources in health care].", "content": "It will be necessary to coordinate the University role with health institutional policies concerning the definition of health goals, and personnel functions as the latter should be trained if and when they are offered the opportunities of grasping both theory and practice in an environment closely related to everyday problems and realities. Accordingly, health care changes must precede health educational changes. There are two factors that interfere with a proper integration between the education care system: 1) an excess number of medical (or health students that overcome teaching facilities and 2) the correct trend in health care, based on specialists, attached to large hospital centers in urban environments. No attention is paid in this way to the importance of the health team, to health promotion, health education, preventive measures, etc. In several Mexican schools new curricula have been developed in which students face actual health problems from the beginning of the studies and they are trained as another resource of the health system being involved in all type of health activities in health centers, schools, nurseries, out-patient community clinics, etc.", "contents": "[Integration of education and services as a base for the formation of human resources in health care]. It will be necessary to coordinate the University role with health institutional policies concerning the definition of health goals, and personnel functions as the latter should be trained if and when they are offered the opportunities of grasping both theory and practice in an environment closely related to everyday problems and realities. Accordingly, health care changes must precede health educational changes. There are two factors that interfere with a proper integration between the education care system: 1) an excess number of medical (or health students that overcome teaching facilities and 2) the correct trend in health care, based on specialists, attached to large hospital centers in urban environments. No attention is paid in this way to the importance of the health team, to health promotion, health education, preventive measures, etc. In several Mexican schools new curricula have been developed in which students face actual health problems from the beginning of the studies and they are trained as another resource of the health system being involved in all type of health activities in health centers, schools, nurseries, out-patient community clinics, etc.", "PMID": 973857}
{"id": "pubmed23n0032_9539", "title": "[A case of intestinal trichobezoar].", "content": "Report is made of a case of an unusual localization of a trichobezoar in a 12 year-old girl, who presented vomiting and abdominal pain as chief complaints. She was found to have an 18 X 5 cm palpable abdominal mass, which upon surgery was localized in terminal ileum and in the pathology report was found to be a trichobezoar. The interest of this case is the unusual localization of a single bezoar and the fact that a resection of 25 cm. of small bowel had to be done, as it was found necrotic at the time of surgery.", "contents": "[A case of intestinal trichobezoar]. Report is made of a case of an unusual localization of a trichobezoar in a 12 year-old girl, who presented vomiting and abdominal pain as chief complaints. She was found to have an 18 X 5 cm palpable abdominal mass, which upon surgery was localized in terminal ileum and in the pathology report was found to be a trichobezoar. The interest of this case is the unusual localization of a single bezoar and the fact that a resection of 25 cm. of small bowel had to be done, as it was found necrotic at the time of surgery.", "PMID": 973856}
{"id": "pubmed23n0032_9540", "title": "[Immunopathological study in membranoproliferative glomerulonephritis].", "content": "In 39 renal percutaneous biopsies, practiced to 31 patients with membranoproliferative glomerulonephritis (GMP) with subendothelial deposits (DSE), a study with immunofluorescence technique and light microscopy was carried out. In all cases, heavy granular deposits of C3 were detected in the loops of the glomerular capillaries and in a variable proportion of cases, deposits of IgM, IgA, C3PA (factor B) and C1q of similar aspect and localizacion were found. These findings suggest immunologic pathogenesis. In the absence of C1q, no cases with factor B were found; thus, it is possible to assert that the activation of the complement system takes place exclusively through an alternate pathway, as was previously accepted. On the other hand, it was found that the presence of C1q was correlated with a faster evolution to chronic renal failure and with the presence of a higher percentage of glomeruli with extracapillary proliferation (crescents). Thus, it is concluded that activation of C1q in patients with MPG and SED, may play a role in formation of crescent and consequently, it is a sign of poor prognosis.", "contents": "[Immunopathological study in membranoproliferative glomerulonephritis]. In 39 renal percutaneous biopsies, practiced to 31 patients with membranoproliferative glomerulonephritis (GMP) with subendothelial deposits (DSE), a study with immunofluorescence technique and light microscopy was carried out. In all cases, heavy granular deposits of C3 were detected in the loops of the glomerular capillaries and in a variable proportion of cases, deposits of IgM, IgA, C3PA (factor B) and C1q of similar aspect and localizacion were found. These findings suggest immunologic pathogenesis. In the absence of C1q, no cases with factor B were found; thus, it is possible to assert that the activation of the complement system takes place exclusively through an alternate pathway, as was previously accepted. On the other hand, it was found that the presence of C1q was correlated with a faster evolution to chronic renal failure and with the presence of a higher percentage of glomeruli with extracapillary proliferation (crescents). Thus, it is concluded that activation of C1q in patients with MPG and SED, may play a role in formation of crescent and consequently, it is a sign of poor prognosis.", "PMID": 973859}
{"id": "pubmed23n0032_9541", "title": "[Sudden infant death syndrome].", "content": "This report is based on a review of the present situation of the sudden infant death syndrome through the presentation of four cases studied at the Unidad de Pediatr\u00eda, Hospital General de M\u00e9xico, S.S.A. All cases were in apparent good health before death. All babies were less than ten months of age. In three cases, necropsy was not performed, and the other one did not show significant abnormalities at the post-mortem examination. A complete review of the literature was made including: historical, epidemiological, genetic, clinical and pathological aspects. Special emphasis is made on the pathophysiology of the syndrome during MOR phase of sleep and muscular hypertrophy of the lungs arteriolae suggesting chronic hypoxia which are the most relevant theories in the sudden infant death syndrome. Psychological aspects and the family management by the physician and detection of possible future victims of the syndrome are finally discussed.", "contents": "[Sudden infant death syndrome]. This report is based on a review of the present situation of the sudden infant death syndrome through the presentation of four cases studied at the Unidad de Pediatr\u00eda, Hospital General de M\u00e9xico, S.S.A. All cases were in apparent good health before death. All babies were less than ten months of age. In three cases, necropsy was not performed, and the other one did not show significant abnormalities at the post-mortem examination. A complete review of the literature was made including: historical, epidemiological, genetic, clinical and pathological aspects. Special emphasis is made on the pathophysiology of the syndrome during MOR phase of sleep and muscular hypertrophy of the lungs arteriolae suggesting chronic hypoxia which are the most relevant theories in the sudden infant death syndrome. Psychological aspects and the family management by the physician and detection of possible future victims of the syndrome are finally discussed.", "PMID": 973858}
{"id": "pubmed23n0032_9542", "title": "[Mechanism of adaptation of the newborn infant].", "content": "An analysis of the mechanisms of adaptation of the newborn infant, immediately after birth, is put forth. Emphasis is placed upon cardiorespiratory and metabolic homeostasis, with a description of the internal medium variations in neonates seeking spontaneous equilibrium. In Mexico City this takes 4 hours. Hypoxia is stressed as one of the most important complications of the neonatal period, requiring rapid and efficient management of the infant to avoid severe complications or even death. Intra and extracardaic hemodynamic changes, stabilization of central arterial pressure as well as sufficient cardiac output, due to proper left ventricle function are described in reference to the cardiovascular sphere. A brief mention is made of the work of both ventricles during the first days of life. The diverse hemodynamic behaviour in different vascular areas of the newborn infant might be due to the concentrations of circulating gases, pH, enzyme factors and circulating hormones.", "contents": "[Mechanism of adaptation of the newborn infant]. An analysis of the mechanisms of adaptation of the newborn infant, immediately after birth, is put forth. Emphasis is placed upon cardiorespiratory and metabolic homeostasis, with a description of the internal medium variations in neonates seeking spontaneous equilibrium. In Mexico City this takes 4 hours. Hypoxia is stressed as one of the most important complications of the neonatal period, requiring rapid and efficient management of the infant to avoid severe complications or even death. Intra and extracardaic hemodynamic changes, stabilization of central arterial pressure as well as sufficient cardiac output, due to proper left ventricle function are described in reference to the cardiovascular sphere. A brief mention is made of the work of both ventricles during the first days of life. The diverse hemodynamic behaviour in different vascular areas of the newborn infant might be due to the concentrations of circulating gases, pH, enzyme factors and circulating hormones.", "PMID": 973860}
{"id": "pubmed23n0032_9543", "title": "[Concentration of lead in the blood of children with hyperkinesis].", "content": "The concentration of lead in bloods of 25 hyperkinetic children was compared to that found in a corresponding number of healthy children of the same age group. It was found that 3 hyperkinetic children showed levels above 40 mug/100 ml. The findings are discussed and the importance of substances containing this element bears on the child's environment are pointed out. Besides, stress is placed on the consequence that a high concentration of lead in the blood may show on mental development and it is suggested that through studies be carried out in every child with the habit of pica added to hyperkinesia.", "contents": "[Concentration of lead in the blood of children with hyperkinesis]. The concentration of lead in bloods of 25 hyperkinetic children was compared to that found in a corresponding number of healthy children of the same age group. It was found that 3 hyperkinetic children showed levels above 40 mug/100 ml. The findings are discussed and the importance of substances containing this element bears on the child's environment are pointed out. Besides, stress is placed on the consequence that a high concentration of lead in the blood may show on mental development and it is suggested that through studies be carried out in every child with the habit of pica added to hyperkinesia.", "PMID": 973862}
{"id": "pubmed23n0032_9544", "title": "[Anhydrotic ectodermal dysplasia].", "content": "The purpose of this paper is to report a boy with a typical case of anhydrotic ecotdermal dysplasia, spina bifida occulat, and abnormal dermatoglyphics. Histologic studies of the skin showed absence of sebaceous and sweat gland and hair follicles. The clinical examination of the mother and the histopathological report of her skin suggests sex linked transmission; however, an autosomal dominat gene limited to males can not be excluded.", "contents": "[Anhydrotic ectodermal dysplasia]. The purpose of this paper is to report a boy with a typical case of anhydrotic ecotdermal dysplasia, spina bifida occulat, and abnormal dermatoglyphics. Histologic studies of the skin showed absence of sebaceous and sweat gland and hair follicles. The clinical examination of the mother and the histopathological report of her skin suggests sex linked transmission; however, an autosomal dominat gene limited to males can not be excluded.", "PMID": 973866}
{"id": "pubmed23n0032_9545", "title": "[Kernicterus. Anatomo clinical correlation in 64 newborn infants].", "content": "The case histories and autopsy reports of 64 neonates who died from kernicterus were reviewed. The most outstanding findings were: high incidence of kernicterus with serum indirect bilirubin less than 15 mg.; different clinical picture of the premature in comparison with term neonate; predisposing factors such as respiratory distress and intrauterine malnutrition were often associated in the premature. Preventive, educational and therapeutic measures were discussed at the end.", "contents": "[Kernicterus. Anatomo clinical correlation in 64 newborn infants]. The case histories and autopsy reports of 64 neonates who died from kernicterus were reviewed. The most outstanding findings were: high incidence of kernicterus with serum indirect bilirubin less than 15 mg.; different clinical picture of the premature in comparison with term neonate; predisposing factors such as respiratory distress and intrauterine malnutrition were often associated in the premature. Preventive, educational and therapeutic measures were discussed at the end.", "PMID": 973861}
{"id": "pubmed23n0032_9546", "title": "[Aglossia-adactilia syndrome].", "content": "A 7 month-old girl was studied and found to have aglossia--adactylia syndrome. The etiopathogenic aspects of this disorder are discussed.", "contents": "[Aglossia-adactilia syndrome]. A 7 month-old girl was studied and found to have aglossia--adactylia syndrome. The etiopathogenic aspects of this disorder are discussed.", "PMID": 973864}
{"id": "pubmed23n0032_9547", "title": "Echocardiographic assessment of abnormal left ventricular relaxation in man.", "content": "In 64 patients requiring cardiac catheterization for chest pain, echocardiograms showing anterior mitral leaflet and left ventricular cavity simultaneously were recorded. These were digitized and their first derivatives computed in order to study time relations between mitral valve and left ventricular wall movement in early distole. In 10 patients with normal left ventricular angiograms and coronary arteriograms, mitral valve opening began 1-1 +/- 9-3 ms (mean +/- SD) before the onset of outward wall movement, and reached peak opening velocity 2-0 +/- 13 ms after maximum rate of change of dimension. Virtually identical time relations were seen in 15 patients with normal left ventricular angiograms but with obstructive coronary artery disease (3-6 +/- 9-3 ms and 0-7 +/- 7-3 ms, respectively). These close relations were lost in patients with segmental abnormalities of contraction on left ventricular angiogram. In 19 such patients with normal septal motion, outward wall movement began 53 +/- 31 ms before the onset of anterior movement of the mitral valve leaflet, and this isovolumic wall movement accounted for 31 per cent of the total diastolic excursion. In 9 patients with reversed septal movement, these abnormalities were greater, 92 +/- 39 ms and 33 per cent, respectively, while in 11 patients with diffuse left ventricular involvement they were small, 5-5 +/- 13 ms and 3 per cent. Frame-by-frame digitization of cineangiograms was used to confirm these findings which appear to reflect an abnormal change in left ventricular cavity shape during isovolumic relaxation.", "contents": "Echocardiographic assessment of abnormal left ventricular relaxation in man. In 64 patients requiring cardiac catheterization for chest pain, echocardiograms showing anterior mitral leaflet and left ventricular cavity simultaneously were recorded. These were digitized and their first derivatives computed in order to study time relations between mitral valve and left ventricular wall movement in early distole. In 10 patients with normal left ventricular angiograms and coronary arteriograms, mitral valve opening began 1-1 +/- 9-3 ms (mean +/- SD) before the onset of outward wall movement, and reached peak opening velocity 2-0 +/- 13 ms after maximum rate of change of dimension. Virtually identical time relations were seen in 15 patients with normal left ventricular angiograms but with obstructive coronary artery disease (3-6 +/- 9-3 ms and 0-7 +/- 7-3 ms, respectively). These close relations were lost in patients with segmental abnormalities of contraction on left ventricular angiogram. In 19 such patients with normal septal motion, outward wall movement began 53 +/- 31 ms before the onset of anterior movement of the mitral valve leaflet, and this isovolumic wall movement accounted for 31 per cent of the total diastolic excursion. In 9 patients with reversed septal movement, these abnormalities were greater, 92 +/- 39 ms and 33 per cent, respectively, while in 11 patients with diffuse left ventricular involvement they were small, 5-5 +/- 13 ms and 3 per cent. Frame-by-frame digitization of cineangiograms was used to confirm these findings which appear to reflect an abnormal change in left ventricular cavity shape during isovolumic relaxation.", "PMID": 973873}
{"id": "pubmed23n0032_9548", "title": "[Etiology of urinary infections].", "content": "The role played by different groups of microorganisms, including aerobic and anaerobic bacteria, viruses, etc., on acute and chronic infections of urinary ducts is discussed, together with general aspects of pathogenesis and diagnosis of such infections.", "contents": "[Etiology of urinary infections]. The role played by different groups of microorganisms, including aerobic and anaerobic bacteria, viruses, etc., on acute and chronic infections of urinary ducts is discussed, together with general aspects of pathogenesis and diagnosis of such infections.", "PMID": 973867}
{"id": "pubmed23n0032_9549", "title": "Analysis of left ventricular wall movement during isovolumic relaxation and its relation to coronary artery disease.", "content": "Left ventricular angiograms of 60 patients with ischaemic heart disease and 10 normal subjects were digitized frame by frame in order to study abnormalities of wall movement during the period of isovolumic relaxation. Plots were made of regional wall movement around the cavity throughout the cardiac cycle. In normal subjects 1-5 to 3-0 mm of symmetrical outward wall movement occurred during isovolumic relaxation, associated with an apparent increase of left ventricular volume of 10 +/- 4 per cent. The corresponding peak velocities of wall movement were 4-3 to 5-7 cm/s, significantly less than those recorded in the same region of the cavity after mitral valve opening. In patients with ischaemic heart disease, the following abnormalities were encountered: (1) Abnormal inward movement, which, in single coronary artery disease, occurred in the area supplied by the affected vessel. (2) Abnormal outward movement of more than 6 mm in non-affected areas which appeared to be a compensatory phenomenon. (3) An abnormal cavity shape change towards a more circular configuration before mitral valve opening. (4) Reduced peak rates of wall movement in affected areas during systole and filling. It is concluded that such inward wall movement during isovolumic relaxation is abnormal and a sign of local ischaemia whose presence has significant effects on overall left ventricular function in both systole and diastole.", "contents": "Analysis of left ventricular wall movement during isovolumic relaxation and its relation to coronary artery disease. Left ventricular angiograms of 60 patients with ischaemic heart disease and 10 normal subjects were digitized frame by frame in order to study abnormalities of wall movement during the period of isovolumic relaxation. Plots were made of regional wall movement around the cavity throughout the cardiac cycle. In normal subjects 1-5 to 3-0 mm of symmetrical outward wall movement occurred during isovolumic relaxation, associated with an apparent increase of left ventricular volume of 10 +/- 4 per cent. The corresponding peak velocities of wall movement were 4-3 to 5-7 cm/s, significantly less than those recorded in the same region of the cavity after mitral valve opening. In patients with ischaemic heart disease, the following abnormalities were encountered: (1) Abnormal inward movement, which, in single coronary artery disease, occurred in the area supplied by the affected vessel. (2) Abnormal outward movement of more than 6 mm in non-affected areas which appeared to be a compensatory phenomenon. (3) An abnormal cavity shape change towards a more circular configuration before mitral valve opening. (4) Reduced peak rates of wall movement in affected areas during systole and filling. It is concluded that such inward wall movement during isovolumic relaxation is abnormal and a sign of local ischaemia whose presence has significant effects on overall left ventricular function in both systole and diastole.", "PMID": 973874}
{"id": "pubmed23n0032_9550", "title": "Acute myocardial infarction. Evaluation of praecordial ST segment mapping.", "content": "In 38 patients with acute anterior myocardial infarction or ischaemia, the extent and amplitude of ST segment elevation was measured on the chest wall with a praecordial mapping technique. There was a poor correlation with measurements of clinical severity and with the extent of necrosis as measured by the peak levels of creatine kinase (CK) and the estimated total amount of CK released. In two patients ST segment re-elevation occurred without re-elevation of serum CK and in two other patients reinfarction obvious on enzyme re-elevation occurred without changes in ST segments. Praecordial ST segment mapping appears to have a limited role in measuring or monitoring human infarct size and would be an unreliable tool for evaluation of methods to limit myocardial necrosis. The chest leads of the standard 12-lead electrocardiogram provided sufficient information for clinical evaluation of ST segment elevation.", "contents": "Acute myocardial infarction. Evaluation of praecordial ST segment mapping. In 38 patients with acute anterior myocardial infarction or ischaemia, the extent and amplitude of ST segment elevation was measured on the chest wall with a praecordial mapping technique. There was a poor correlation with measurements of clinical severity and with the extent of necrosis as measured by the peak levels of creatine kinase (CK) and the estimated total amount of CK released. In two patients ST segment re-elevation occurred without re-elevation of serum CK and in two other patients reinfarction obvious on enzyme re-elevation occurred without changes in ST segments. Praecordial ST segment mapping appears to have a limited role in measuring or monitoring human infarct size and would be an unreliable tool for evaluation of methods to limit myocardial necrosis. The chest leads of the standard 12-lead electrocardiogram provided sufficient information for clinical evaluation of ST segment elevation.", "PMID": 973875}
{"id": "pubmed23n0032_9551", "title": "[Intravenous and oral glucose: production of insulin and growth hormone in the newborn infant].", "content": "Insulin production in response to intravenous and high and low oral glucose administration was investigated in 15 healthy fasting newborns. Results show that in spite of the fact that the highest blood glucose was obtained by the intravenous load, the highest insulin levels corresponded to the high oral glucose. The lowest blood glucose levels were found in the cases who received the low oral doses. The amounts of circulating insulin in this group were not significantly different from those in the group with I.V. glucose. In two of the groups where growth hormone was measured, the known paradoxixal increase in response to administered glucose was observed.", "contents": "[Intravenous and oral glucose: production of insulin and growth hormone in the newborn infant]. Insulin production in response to intravenous and high and low oral glucose administration was investigated in 15 healthy fasting newborns. Results show that in spite of the fact that the highest blood glucose was obtained by the intravenous load, the highest insulin levels corresponded to the high oral glucose. The lowest blood glucose levels were found in the cases who received the low oral doses. The amounts of circulating insulin in this group were not significantly different from those in the group with I.V. glucose. In two of the groups where growth hormone was measured, the known paradoxixal increase in response to administered glucose was observed.", "PMID": 973871}
{"id": "pubmed23n0032_9552", "title": "Effect of potassium-sparing diuretics on the renin-angiotensin-aldosterone system and potassium retention in heart failure.", "content": "The renin-angiotensin-aldosterone system and electrolyte levels in 11 patients with heart failure controlled on digoxin and frusemide were investigated after separate periods of Slow K, spironolactone, and amiloride therapy. When spironolactone or amiloride replaced Slow K, distinct parallel increments in the levels of renin, angiotensin II, and aldosterone resulted. Though plasma potassium was generally higher after spironolactone and amiloride than after Slow K, exchangeable potassium was similar with the three regimens. There was no significant relation between plasma potassium and concurrent exchangeable potassium.", "contents": "Effect of potassium-sparing diuretics on the renin-angiotensin-aldosterone system and potassium retention in heart failure. The renin-angiotensin-aldosterone system and electrolyte levels in 11 patients with heart failure controlled on digoxin and frusemide were investigated after separate periods of Slow K, spironolactone, and amiloride therapy. When spironolactone or amiloride replaced Slow K, distinct parallel increments in the levels of renin, angiotensin II, and aldosterone resulted. Though plasma potassium was generally higher after spironolactone and amiloride than after Slow K, exchangeable potassium was similar with the three regimens. There was no significant relation between plasma potassium and concurrent exchangeable potassium.", "PMID": 973876}
{"id": "pubmed23n0032_9553", "title": "Haemodynamics after Mustard's operation for transposition of the great arteries.", "content": "Cardiac catheterization data from 54 investigations after Mustard's procedure were examined to study the influence of the operation on pressure events in the atria, great veins, and pulmonary circulation. Systemic venous atrial pressure tracings were characterized by a rapid, sharp 'y' descent. Pressure gradients between the venae cavae and systemic venous atrium were invariable, whether or not vena caval pathway obstruction was present, the 'y' trough and 'a' wave gradients being greater than the mean gradient. Pulmonary venous atrial pressure tracings were not different from normal except when tricuspid regurgitation was present. It is suggested that the baffle effectively reduces the size and compliance of the systemic venous atrium, but influences the pulmonary venous atrium to a lesser degree. The systolic pressure gradient from the left ventricle to pulmonary artery was decreased postoperatively, suggesting that it may be flow-related; the greatest changes were seen in the group with preoperative ventricular septal defect. The ratio of pulmonary: systemic vascular resistance did not change significantly after operation, and it is suggested that both the pre- and postoperative values were higher than normal. Examination of the left ventricular or pulmonary arterial mean pressure postoperatively should raise the suspicion of a complication, e.g. pulmonary venous obstruction or tricuspid regurgitation.", "contents": "Haemodynamics after Mustard's operation for transposition of the great arteries. Cardiac catheterization data from 54 investigations after Mustard's procedure were examined to study the influence of the operation on pressure events in the atria, great veins, and pulmonary circulation. Systemic venous atrial pressure tracings were characterized by a rapid, sharp 'y' descent. Pressure gradients between the venae cavae and systemic venous atrium were invariable, whether or not vena caval pathway obstruction was present, the 'y' trough and 'a' wave gradients being greater than the mean gradient. Pulmonary venous atrial pressure tracings were not different from normal except when tricuspid regurgitation was present. It is suggested that the baffle effectively reduces the size and compliance of the systemic venous atrium, but influences the pulmonary venous atrium to a lesser degree. The systolic pressure gradient from the left ventricle to pulmonary artery was decreased postoperatively, suggesting that it may be flow-related; the greatest changes were seen in the group with preoperative ventricular septal defect. The ratio of pulmonary: systemic vascular resistance did not change significantly after operation, and it is suggested that both the pre- and postoperative values were higher than normal. Examination of the left ventricular or pulmonary arterial mean pressure postoperatively should raise the suspicion of a complication, e.g. pulmonary venous obstruction or tricuspid regurgitation.", "PMID": 973877}
{"id": "pubmed23n0032_9554", "title": "[Teratoma of the thyroid gland in children].", "content": "Two cases of teratoma of the thyroid gland occurring in children are presented. Both tumors were discovered shortly after birth and were not associated with clinical hypothyroidism. One of the patients was thoroughly studied from the endocrinological point of view utilizing several techniques. There was some iodine uptake by the gland, formation of tirosine and thyroid hormones as well as their release into the blood serum. The clinical and pathological features of teratoma of the thyroid are discussed.", "contents": "[Teratoma of the thyroid gland in children]. Two cases of teratoma of the thyroid gland occurring in children are presented. Both tumors were discovered shortly after birth and were not associated with clinical hypothyroidism. One of the patients was thoroughly studied from the endocrinological point of view utilizing several techniques. There was some iodine uptake by the gland, formation of tirosine and thyroid hormones as well as their release into the blood serum. The clinical and pathological features of teratoma of the thyroid are discussed.", "PMID": 973872}
{"id": "pubmed23n0032_9555", "title": "Ventricular arrhythmias in syndrome of balloon deformity of mitral valve. Definition of possible high risk group.", "content": "Twenty patients clinically identified as having balloon deformity of the mitral valve were studied to assess the incidence of ventricular arrhythmias. Echocardiography and phonocardiography were used to confirm the nature of the mitral valve lesion. Continuous 24-hour electrocardiograms were obtained from all patients and analysed by a computer and 2 observers. One patient has ventricular fibrillation and 3 patients had ventricular tachycardia. There was a high incidence of other less severe forms of ventricular arrhythmias. Eight patients had inferolateral ST and T wave abnormality on the resting electrocardiogram, and were described as having the ausculatatory-electrocardiographic variant of the balloon mitral valve syndrome. The occurrence of serious ventricular arrhythmias (ventricular fibrillation and tachycardia) was significantly more frequent in this group. This raises the possibility that the resting electrocardiogram may identify those patients with balloon deformity of the mitral valve who are at risk from sudden death.", "contents": "Ventricular arrhythmias in syndrome of balloon deformity of mitral valve. Definition of possible high risk group. Twenty patients clinically identified as having balloon deformity of the mitral valve were studied to assess the incidence of ventricular arrhythmias. Echocardiography and phonocardiography were used to confirm the nature of the mitral valve lesion. Continuous 24-hour electrocardiograms were obtained from all patients and analysed by a computer and 2 observers. One patient has ventricular fibrillation and 3 patients had ventricular tachycardia. There was a high incidence of other less severe forms of ventricular arrhythmias. Eight patients had inferolateral ST and T wave abnormality on the resting electrocardiogram, and were described as having the ausculatatory-electrocardiographic variant of the balloon mitral valve syndrome. The occurrence of serious ventricular arrhythmias (ventricular fibrillation and tachycardia) was significantly more frequent in this group. This raises the possibility that the resting electrocardiogram may identify those patients with balloon deformity of the mitral valve who are at risk from sudden death.", "PMID": 973878}
{"id": "pubmed23n0032_9556", "title": "Ventriculo-atrial conduction time during reciprocating tachycardia with intermittent bundle-branch block in Wolff-Parkinson-White syndrome.", "content": "Records from patients with the Wolff-Parkinson-White syndrome were reviewed with particular emphasis on the occurrence of bundle-branch block aberration during reciprocating tachycardia and the significance of this observation with respect to accessory pathway location. Increase by greater than 25 ms in the ventriculoatrial interval during reciprocating tachycardia with bundle-branch block, when compared to reciprocating tachycardia with normal intraventricular conduction, occurred only with right or left free wall accessory pathways. No patient with a septal accessory pathway proven by epicardial mapping showed a ventriculoatrial interval prolongation greater than 20 ms during bundle-branch block aberration. Measurement of ventriculo-atrial interval during bundle-branch block abe-ration also helped to diagnose accessory pathways AH and HV intervals as well as ventriculo-atrial times, may give midleading information. In one patient increase in cycle length during left bundle-branch block was the result of prolonged HV interval rather than prolonged ventriculo-atrial interval. In another patient cycle length remained the same during bundle-branch block while the ventriculo-atrial interval increased by an increment identical to the decrease in AH interval.", "contents": "Ventriculo-atrial conduction time during reciprocating tachycardia with intermittent bundle-branch block in Wolff-Parkinson-White syndrome. Records from patients with the Wolff-Parkinson-White syndrome were reviewed with particular emphasis on the occurrence of bundle-branch block aberration during reciprocating tachycardia and the significance of this observation with respect to accessory pathway location. Increase by greater than 25 ms in the ventriculoatrial interval during reciprocating tachycardia with bundle-branch block, when compared to reciprocating tachycardia with normal intraventricular conduction, occurred only with right or left free wall accessory pathways. No patient with a septal accessory pathway proven by epicardial mapping showed a ventriculoatrial interval prolongation greater than 20 ms during bundle-branch block aberration. Measurement of ventriculo-atrial interval during bundle-branch block abe-ration also helped to diagnose accessory pathways AH and HV intervals as well as ventriculo-atrial times, may give midleading information. In one patient increase in cycle length during left bundle-branch block was the result of prolonged HV interval rather than prolonged ventriculo-atrial interval. In another patient cycle length remained the same during bundle-branch block while the ventriculo-atrial interval increased by an increment identical to the decrease in AH interval.", "PMID": 973879}
{"id": "pubmed23n0032_9557", "title": "Developing cardiac rupture as initial sign of acute myocardial infarction.", "content": "Pathological data are given of 4 patients in whom the development of cardiac rupture was the initial sign of myocardial infarction. The term 'concealed cardiac rupture' is proposed for this condition. In each instance the clinical diagnosis was 'onset of infarction', whereas the necropsy suggested that the acute onset of symptoms was caused by the start of rupture. The arguments for classifying the cases as 'concealed rupture' are (1) the lack of correlation between histological and clinical dating of the infarct, (2) the observation that the tear may take considerable time before actual epicardial breakthrough occurs, and (3) the fact that a high percentage of infarcts may pass clinically unnoticed.", "contents": "Developing cardiac rupture as initial sign of acute myocardial infarction. Pathological data are given of 4 patients in whom the development of cardiac rupture was the initial sign of myocardial infarction. The term 'concealed cardiac rupture' is proposed for this condition. In each instance the clinical diagnosis was 'onset of infarction', whereas the necropsy suggested that the acute onset of symptoms was caused by the start of rupture. The arguments for classifying the cases as 'concealed rupture' are (1) the lack of correlation between histological and clinical dating of the infarct, (2) the observation that the tear may take considerable time before actual epicardial breakthrough occurs, and (3) the fact that a high percentage of infarcts may pass clinically unnoticed.", "PMID": 973881}
{"id": "pubmed23n0032_9558", "title": "Annular subvalvular left ventricular aneurysm in Bahia, Brazil.", "content": "Two cases of left ventricular aneurysm, a 16-year-old black boy and a 23-year-old white girl, from Bahia, Brazil, are presented. In both patients there was enlargement of the cardiac silhouette and a prominent bulge of the left inferior border. On the right oblique view a ring of calcium at the ventricular opening of the aneurysms was visualized. A left ventriculogram showed a huge aneurysm in the first case and a bulge on the lateral wall of the left ventricle in the other. Cardiac catheterization showed a rise in left and right ventricular end-diastolic pressures and in the mean pulmonary artery pressure. In the first case the contour of the right ventricular pressure curve showed a restrictive pattern. The similarities of these aneurysms with the annular submitral type described in young black Africans are stressed.", "contents": "Annular subvalvular left ventricular aneurysm in Bahia, Brazil. Two cases of left ventricular aneurysm, a 16-year-old black boy and a 23-year-old white girl, from Bahia, Brazil, are presented. In both patients there was enlargement of the cardiac silhouette and a prominent bulge of the left inferior border. On the right oblique view a ring of calcium at the ventricular opening of the aneurysms was visualized. A left ventriculogram showed a huge aneurysm in the first case and a bulge on the lateral wall of the left ventricle in the other. Cardiac catheterization showed a rise in left and right ventricular end-diastolic pressures and in the mean pulmonary artery pressure. In the first case the contour of the right ventricular pressure curve showed a restrictive pattern. The similarities of these aneurysms with the annular submitral type described in young black Africans are stressed.", "PMID": 973882}
{"id": "pubmed23n0032_9559", "title": "Pulmonary hydatid embolization. Report on 2 operated cases and review of published reports.", "content": "Two patients with pulmonary hydatid embolization are described and commented upon in the light of 43 similar published cases. The diagnosis was strongly suspected from the medical history and the chest x-ray films and supported by angiocardiography. The angiocardiographic features of this condition have not been described previously in detail. They include amputation and filling defects of pulmonary artery branches, which are typically located proximal to a rounded tumour-like opacity seen on the plain x-ray film. Both patients underwent successful embolectomy.", "contents": "Pulmonary hydatid embolization. Report on 2 operated cases and review of published reports. Two patients with pulmonary hydatid embolization are described and commented upon in the light of 43 similar published cases. The diagnosis was strongly suspected from the medical history and the chest x-ray films and supported by angiocardiography. The angiocardiographic features of this condition have not been described previously in detail. They include amputation and filling defects of pulmonary artery branches, which are typically located proximal to a rounded tumour-like opacity seen on the plain x-ray film. Both patients underwent successful embolectomy.", "PMID": 973883}
{"id": "pubmed23n0032_9560", "title": "Massive pulmonary arteriovenous fistula in the newborn.", "content": "Although pulmonary arteriovenous fistula as a cause of cyanosis is well recognized, most of the reported cases occur in older children and adults, and its importance as a correctable lesion in the newborn is often overlooked. The details of two babies who presented with cyanosis in the first few days of life are presented to emphasize that this eminently treatable lesion may need to be managed as an emergency.", "contents": "Massive pulmonary arteriovenous fistula in the newborn. Although pulmonary arteriovenous fistula as a cause of cyanosis is well recognized, most of the reported cases occur in older children and adults, and its importance as a correctable lesion in the newborn is often overlooked. The details of two babies who presented with cyanosis in the first few days of life are presented to emphasize that this eminently treatable lesion may need to be managed as an emergency.", "PMID": 973884}
{"id": "pubmed23n0032_9561", "title": "Muscle potentials simulating pacemaker malfunction.", "content": "Interference spikes were noted on the electrocardiogram of a patient with an implanted demand pacemaker. Runaway malfunction was suspected and the generator replaced. Subsequent investigation showec that the interference originated from fasciculation in the left leg caused by underlying neuromuscular disease. Skeletal muscle potentials can produce an electrocardiographic appearance closely resembling 'runaway' pacemaker. Such abnormalities should prompt a search for occult neuromuscular disease.", "contents": "Muscle potentials simulating pacemaker malfunction. Interference spikes were noted on the electrocardiogram of a patient with an implanted demand pacemaker. Runaway malfunction was suspected and the generator replaced. Subsequent investigation showec that the interference originated from fasciculation in the left leg caused by underlying neuromuscular disease. Skeletal muscle potentials can produce an electrocardiographic appearance closely resembling 'runaway' pacemaker. Such abnormalities should prompt a search for occult neuromuscular disease.", "PMID": 973885}
{"id": "pubmed23n0032_9562", "title": "Electrocardiographic changes produced by interventricular septal rupture.", "content": "The electrocardiogram was recorded during spontaneous rupture of the interventricular septum in a patient after myocardial infarction. The electrocardiographic abnormalities included an increase in the sinus rate, elevation of the ST segment, decrease in Q wave size, and increase in the height and width of the P wave.", "contents": "Electrocardiographic changes produced by interventricular septal rupture. The electrocardiogram was recorded during spontaneous rupture of the interventricular septum in a patient after myocardial infarction. The electrocardiographic abnormalities included an increase in the sinus rate, elevation of the ST segment, decrease in Q wave size, and increase in the height and width of the P wave.", "PMID": 973886}
{"id": "pubmed23n0032_9563", "title": "Improvement in exertional left ventricular dysfunction after revascularization.", "content": "The case is reported of a 63-year-old white man with mild angina pectoris, whose systolic pressure fell 30 mmHg (4-0 kPa) with maximal exercise, without chest pain but with accompanying dizziness. Grafting the internal mammary arteries into the mid left anterior descending and obtuse marginal arteries improved regional myocardial perfusion and increased maximal cardiac output 24 per cent and maximal systolic pressure 32 per cent.", "contents": "Improvement in exertional left ventricular dysfunction after revascularization. The case is reported of a 63-year-old white man with mild angina pectoris, whose systolic pressure fell 30 mmHg (4-0 kPa) with maximal exercise, without chest pain but with accompanying dizziness. Grafting the internal mammary arteries into the mid left anterior descending and obtuse marginal arteries improved regional myocardial perfusion and increased maximal cardiac output 24 per cent and maximal systolic pressure 32 per cent.", "PMID": 973887}
{"id": "pubmed23n0032_9564", "title": "Pathology of acute myocardial infarction with particular reference to occlusive coronary thrombi.", "content": "Analysis of the pathological findings in 500 cases of fatal acute myocardial infarction showed that in 469 this was localized to one transmural area of the left ventricle; in 31 there was diffuse subendocardial necrosis. In the former occlusive coronary thrombus was found in the related artery in 95 per cent of cases. Variation in the percentage of occlusions found was noted between different prosectors and when coronary artery calcification was present. Only 4 of the 31 patients with subendocardial necrosis had recent occlusion; triple vessel disease was common in this group suggesting general failure of coronary perfusion. It is essential in necropsy studies of the relation of coronary thrombosis to myocardial infarction to be sure that muscle necrosis is present, to distinguish the two forms of myocardial necrosis, and to employ a meticulous dissection technique with decalcification of the arteries when necessary.", "contents": "Pathology of acute myocardial infarction with particular reference to occlusive coronary thrombi. Analysis of the pathological findings in 500 cases of fatal acute myocardial infarction showed that in 469 this was localized to one transmural area of the left ventricle; in 31 there was diffuse subendocardial necrosis. In the former occlusive coronary thrombus was found in the related artery in 95 per cent of cases. Variation in the percentage of occlusions found was noted between different prosectors and when coronary artery calcification was present. Only 4 of the 31 patients with subendocardial necrosis had recent occlusion; triple vessel disease was common in this group suggesting general failure of coronary perfusion. It is essential in necropsy studies of the relation of coronary thrombosis to myocardial infarction to be sure that muscle necrosis is present, to distinguish the two forms of myocardial necrosis, and to employ a meticulous dissection technique with decalcification of the arteries when necessary.", "PMID": 973888}
{"id": "pubmed23n0032_9565", "title": "Congenital aortic valve disease with rupture of mitral chordae tendineae.", "content": "A new clinical entity is described in which free aortic regurgitation from congenital aortic valve disease caused rupture of the chordae to the anterior leaflet of the mitral valve in 7 men aged 45 to 63 years (mean 52 years); 2 of the patients also had rupture of chordae to the posterior leaflet. Comparing these patients with those with ruptured mitral chordae in  association with rheumatic heart disease and patients with spontaneous chordal rupture, differences were evident. No patient had a history of rheumatic fever and none had active infection. The typical clinical presentation was of acute mitral regurgitation into a small left atrium, with severe pulmonary oedema which was often resistant to medical treatment. The cause of chordal rupture in these patients was in part the result of progressive left ventricular dilatation, of direct trauma to the anterior cusp of the mitral valve, and possibly of a genetic factor. The anatomical features of both aortic and mitral valves are described, and in 3 histology of the mitral valve was available; 2 had myxomatous degeneration similar to that seen in patients with spontaneous chordal rupture, and in 1 there was degeneration of collagen tissue. All patients were treated surgically but the mortality was high (5 out of 7,70%). Early operation with replacement of the aortic and mitral valves is recommended if this high mortality is to be reduced.", "contents": "Congenital aortic valve disease with rupture of mitral chordae tendineae. A new clinical entity is described in which free aortic regurgitation from congenital aortic valve disease caused rupture of the chordae to the anterior leaflet of the mitral valve in 7 men aged 45 to 63 years (mean 52 years); 2 of the patients also had rupture of chordae to the posterior leaflet. Comparing these patients with those with ruptured mitral chordae in  association with rheumatic heart disease and patients with spontaneous chordal rupture, differences were evident. No patient had a history of rheumatic fever and none had active infection. The typical clinical presentation was of acute mitral regurgitation into a small left atrium, with severe pulmonary oedema which was often resistant to medical treatment. The cause of chordal rupture in these patients was in part the result of progressive left ventricular dilatation, of direct trauma to the anterior cusp of the mitral valve, and possibly of a genetic factor. The anatomical features of both aortic and mitral valves are described, and in 3 histology of the mitral valve was available; 2 had myxomatous degeneration similar to that seen in patients with spontaneous chordal rupture, and in 1 there was degeneration of collagen tissue. All patients were treated surgically but the mortality was high (5 out of 7,70%). Early operation with replacement of the aortic and mitral valves is recommended if this high mortality is to be reduced.", "PMID": 973889}
{"id": "pubmed23n0032_9566", "title": "Endocardial cushion defects: an anatomical study of 54 specimens.", "content": "An anatomical study of 54 specimens with endocardial cushion defect is described. The material was divided into two groups, according to the morphology and attachments of the anterior component of the anteroseptal mitral leaflet. We suggest that there is no divided or undivided anterior common leaflet. There are always two anterior leaflets, a mitral and a tricuspid one, separated by a commissure; depending on the degree of straddling of the mitral component over the interventricular septum, this will protrude into the interventricular septum or into the anterior papillary muscle of the right ventricle. Thus, the commissure separating the mitral and the tricuspid components will lie over the interventricular septum or will be quite separate in the right ventricle. In the latter case the straddling mitral component gives the false appearance of an undivided anterior common leaflet. The septal tricuspid leaflet was found to be underdeveloped or absent but we were not able to find any clefts or divisions in it. We suggest that this leaflet derives solely from the division of the posteroinferior endocardial cushion into two halves, right and left. The left half later becomes the posterior component of the normal anteroseptal mitral leaflet. Associated anamalies are also reviewed.", "contents": "Endocardial cushion defects: an anatomical study of 54 specimens. An anatomical study of 54 specimens with endocardial cushion defect is described. The material was divided into two groups, according to the morphology and attachments of the anterior component of the anteroseptal mitral leaflet. We suggest that there is no divided or undivided anterior common leaflet. There are always two anterior leaflets, a mitral and a tricuspid one, separated by a commissure; depending on the degree of straddling of the mitral component over the interventricular septum, this will protrude into the interventricular septum or into the anterior papillary muscle of the right ventricle. Thus, the commissure separating the mitral and the tricuspid components will lie over the interventricular septum or will be quite separate in the right ventricle. In the latter case the straddling mitral component gives the false appearance of an undivided anterior common leaflet. The septal tricuspid leaflet was found to be underdeveloped or absent but we were not able to find any clefts or divisions in it. We suggest that this leaflet derives solely from the division of the posteroinferior endocardial cushion into two halves, right and left. The left half later becomes the posterior component of the normal anteroseptal mitral leaflet. Associated anamalies are also reviewed.", "PMID": 973890}
{"id": "pubmed23n0032_9567", "title": "Influence of heart rate increase on uncorrected pre-ejection period/left ventricular ejection time (PEP/LVET) ratio in normal individuals.", "content": "In 26 normal volunteers, increase in heart rate from mean 73-94 +/- 1-97 to 103-61 +/- 2-72/min, by either intravenous atropine administration or rapid right atrial pacing, produced definite changes in the uncorrected systolic time intervals. As expected, total electromechanical systole (QS2) and left ventricular ejection time (LVET) were shortened, while the pre-ejection period (PEP) was unaffected. There was a consistent and significant increase of the PEP/LVET ratio (P less than 0-001). It is postulated that when this ratio is taken to express left ventricular contractility, it should probably be corrected for heart rate. Appropriate regression equations for such a correction were calculated (PEP/LVET=0-249 + 0-0168 HR).", "contents": "Influence of heart rate increase on uncorrected pre-ejection period/left ventricular ejection time (PEP/LVET) ratio in normal individuals. In 26 normal volunteers, increase in heart rate from mean 73-94 +/- 1-97 to 103-61 +/- 2-72/min, by either intravenous atropine administration or rapid right atrial pacing, produced definite changes in the uncorrected systolic time intervals. As expected, total electromechanical systole (QS2) and left ventricular ejection time (LVET) were shortened, while the pre-ejection period (PEP) was unaffected. There was a consistent and significant increase of the PEP/LVET ratio (P less than 0-001). It is postulated that when this ratio is taken to express left ventricular contractility, it should probably be corrected for heart rate. Appropriate regression equations for such a correction were calculated (PEP/LVET=0-249 + 0-0168 HR).", "PMID": 973891}
{"id": "pubmed23n0032_9568", "title": "Plasma urea in hypertensive patients.", "content": "The annual increase in plasma urea was measured in 253 hypertensive patients. On average there was a significant increase in plasma urea with time which did not depend on the sex of the patient or the type of hypertension. It did, however, depend on the initial level of plasma urea. A table giving the upper limits for expected annual increment may prove useful in clinical assessment. The relation between plasma urea and presenting blood pressure and age was examined in 1217 patients seen at the Hammersmith Hospital hypertension clinic from 1952 to 1967. The plasma urea was significantly related to both age and diastolic and systolic blood pressure. It was higher in men than in women up to 60 years of age, but not above that age, and it increased with presenting mean blood pressure in both sexes, but the increase was greater in men. There was a quadratic relation between age and plasma urea in both men and women. In both sexes the plasma urea increased between the ages of 60 and 80.", "contents": "Plasma urea in hypertensive patients. The annual increase in plasma urea was measured in 253 hypertensive patients. On average there was a significant increase in plasma urea with time which did not depend on the sex of the patient or the type of hypertension. It did, however, depend on the initial level of plasma urea. A table giving the upper limits for expected annual increment may prove useful in clinical assessment. The relation between plasma urea and presenting blood pressure and age was examined in 1217 patients seen at the Hammersmith Hospital hypertension clinic from 1952 to 1967. The plasma urea was significantly related to both age and diastolic and systolic blood pressure. It was higher in men than in women up to 60 years of age, but not above that age, and it increased with presenting mean blood pressure in both sexes, but the increase was greater in men. There was a quadratic relation between age and plasma urea in both men and women. In both sexes the plasma urea increased between the ages of 60 and 80.", "PMID": 973892}
{"id": "pubmed23n0032_9569", "title": "Circulatory response to vasodilator therapy in congestive cardiomyopathy.", "content": "Vasodilator therapy has been shown to have beneficial effects in heart failure. In order to evaluate the haemodynamic actions of vasodilator administration in primary congestive cardiomyopathy, sodium nitroprusside was infused intravenously at a rate of 15 to 100 mug/min to 12 patients. Mean arterial pressure fell 15 per cent from 86+/-3-0 to 72+/-2-4 mmHg (11-40 +/- -4 to 9-6 +/- 0-3 kPa), and there was a small but significant decrease in mean heart rate from 96 +/- 4-8 to 90 +/- 4-4 beats/min. These changes were accompanied by a significant decrease in mean pulmonary artery pressure from 40 +/- 2-2 to 26 +/- 2-8 mmHg (5-3 +/- 0-3 kPa to 3-5 +/- 0-4 kPa), mean pulmonary capillary wedge pressure from 25 +/ -2-2 to 16 +/- 2-1 mmHg (3-3 +/- 0-3 to 2-1 +/- 0-3 kPa), and left ventricular end-diastolic pressure from 27 +/- 1-8 to 17 +/- 1-5 mmHg (3-6 +/- 0-3 to 2-3 +/- 0-2 kPa). Cardiac index increased by an average of 48 per cent from 2-1 to 3-1 l/min per m2, and left ventricular stroke work index increased from 18-4 +/- 1-6 to 21-3 +/- 1-9 g m/m2. These results show that pronounced left ventricular dysfunction in patients with congestive cardiomyopathy is improved during vasodilator therapy.", "contents": "Circulatory response to vasodilator therapy in congestive cardiomyopathy. Vasodilator therapy has been shown to have beneficial effects in heart failure. In order to evaluate the haemodynamic actions of vasodilator administration in primary congestive cardiomyopathy, sodium nitroprusside was infused intravenously at a rate of 15 to 100 mug/min to 12 patients. Mean arterial pressure fell 15 per cent from 86+/-3-0 to 72+/-2-4 mmHg (11-40 +/- -4 to 9-6 +/- 0-3 kPa), and there was a small but significant decrease in mean heart rate from 96 +/- 4-8 to 90 +/- 4-4 beats/min. These changes were accompanied by a significant decrease in mean pulmonary artery pressure from 40 +/- 2-2 to 26 +/- 2-8 mmHg (5-3 +/- 0-3 kPa to 3-5 +/- 0-4 kPa), mean pulmonary capillary wedge pressure from 25 +/ -2-2 to 16 +/- 2-1 mmHg (3-3 +/- 0-3 to 2-1 +/- 0-3 kPa), and left ventricular end-diastolic pressure from 27 +/- 1-8 to 17 +/- 1-5 mmHg (3-6 +/- 0-3 to 2-3 +/- 0-2 kPa). Cardiac index increased by an average of 48 per cent from 2-1 to 3-1 l/min per m2, and left ventricular stroke work index increased from 18-4 +/- 1-6 to 21-3 +/- 1-9 g m/m2. These results show that pronounced left ventricular dysfunction in patients with congestive cardiomyopathy is improved during vasodilator therapy.", "PMID": 973893}
{"id": "pubmed23n0032_9570", "title": "Acute massive mitral regurgitation from prosthetic valve dysfunction.", "content": "Two cases of prosthetic valve dysfunction resulting in acute massive mitral regurgitation are reported; emergency operation was successful in both cases. Survival following complete dislodgement of the occluder of a disc valve, as occurred in one case, does not appear to have been reported before. The diffculty in diagnosis of sudden cardiac decompensation in patients with prosthetic valves is stressed, as is the need for urgent operation.", "contents": "Acute massive mitral regurgitation from prosthetic valve dysfunction. Two cases of prosthetic valve dysfunction resulting in acute massive mitral regurgitation are reported; emergency operation was successful in both cases. Survival following complete dislodgement of the occluder of a disc valve, as occurred in one case, does not appear to have been reported before. The diffculty in diagnosis of sudden cardiac decompensation in patients with prosthetic valves is stressed, as is the need for urgent operation.", "PMID": 973894}
{"id": "pubmed23n0032_9571", "title": "Retrograde conduction in complete atrioventricular block Study using His bundle recordings.", "content": "Using His bundle recordings and ventricular stimulation retrograde conduction was studied in 57 cases of complete AV block. VA conduction was observed in 13 patients. Of the 13, 12 had AV block distal to H, and during cardiac pacing 5 of them showed a retrograde His bundle potential. In all cases the AV node-His pathway was implicated in the transmission of the retrograde impulse. There was no retrograde atrial response in the remaining patients. VA block usually occurred at the same level as AV block. Nevertheless, two cases of AV block distal to H were associated with concealed retrograde conduction which stopped within the AV node.", "contents": "Retrograde conduction in complete atrioventricular block Study using His bundle recordings. Using His bundle recordings and ventricular stimulation retrograde conduction was studied in 57 cases of complete AV block. VA conduction was observed in 13 patients. Of the 13, 12 had AV block distal to H, and during cardiac pacing 5 of them showed a retrograde His bundle potential. In all cases the AV node-His pathway was implicated in the transmission of the retrograde impulse. There was no retrograde atrial response in the remaining patients. VA block usually occurred at the same level as AV block. Nevertheless, two cases of AV block distal to H were associated with concealed retrograde conduction which stopped within the AV node.", "PMID": 973895}
{"id": "pubmed23n0032_9572", "title": "Echocardiographic findings after acute carbon monoxide poisoning.", "content": "Myocardial lesions are frequently seen at necropsy after fatal carbon monoxide poisoning. Clinically, while there have been numerous reports of chest pain and electrocardiographic changes associated with acute carbon monoxide poisoning, other evidence for left ventricular abnormality has not been reported. The echocardiographic findings in five cases of non-fatal poisoning are presented here. Abnormal left ventricular wall motion was shown by echocardiography in 3 cases. Motion returned to normal in 2 of the 3 in follow-up tracings. Echocardiograms on 3 of the 5 patients showed mitral valve prolapse. Though the mitral valve prolapse may have been present before the poisoning, the reported high incidence of papillary muscle lesions in fatal cases suggests a possible relation of the prolapse to the effects of the carbon monoxide poisoning.", "contents": "Echocardiographic findings after acute carbon monoxide poisoning. Myocardial lesions are frequently seen at necropsy after fatal carbon monoxide poisoning. Clinically, while there have been numerous reports of chest pain and electrocardiographic changes associated with acute carbon monoxide poisoning, other evidence for left ventricular abnormality has not been reported. The echocardiographic findings in five cases of non-fatal poisoning are presented here. Abnormal left ventricular wall motion was shown by echocardiography in 3 cases. Motion returned to normal in 2 of the 3 in follow-up tracings. Echocardiograms on 3 of the 5 patients showed mitral valve prolapse. Though the mitral valve prolapse may have been present before the poisoning, the reported high incidence of papillary muscle lesions in fatal cases suggests a possible relation of the prolapse to the effects of the carbon monoxide poisoning.", "PMID": 973896}
{"id": "pubmed23n0032_9573", "title": "Non-ejection systolic clicks and mitral systolic murmurs in black schoolchildren of Soweto, Johannesburg.", "content": "A survey was conducted on 12 050 Black schoolchildren, aged 2 to 18 years, in the South Western Townships of Johannesburg (Soweto), and the prevalence of non-ejection systolic clicks and late systolic murmurs was determined. One or both of these auscultatory findings were detected in 168 children, yielding a prevalence rate of 13-99 per 1000 in the school population. A female preponderance of 1-9:1 was present and there was a strong linear increase in prevalence with age, with a peak rate of 29-41 per 1000 in 17-year-old children. A non-ejection click was the only abnormal auscultatory finding in 123 children (73%) and a mitral systolic murmur in 8 (5%), whereas in 37 (22%) both these findings were present. Of the latter 37 children, the murmur was late systolic in 32; in 5 it was early systolic. Auscultation in different postures was important in the detection of both non-ejection clicks and mitral systolic murmurs. Experience in the detection of these auscultatory findings influenced the frequency with which they were heard. Electrocardiographic abnormalities compatible with those previously described in the billowing mitral leaflet syndrome were present in 11 of 158 children. The aetiology of these auscultatory findings in this community remains unknown. In the same survey, a high prevalence rate of rheumatic heart disease was recorded and the epidemiology of the non-ejection clicks and these mitral systolic murmurs showed similarties to that of rheumatic heart disease. Though the specific billowing mitral leaflet syndrome almost certainly accounts for some of these auscultatory findings, a significant proportion may have early rheumatic heart disease. Further elucidation of this problem is necessary.", "contents": "Non-ejection systolic clicks and mitral systolic murmurs in black schoolchildren of Soweto, Johannesburg. A survey was conducted on 12 050 Black schoolchildren, aged 2 to 18 years, in the South Western Townships of Johannesburg (Soweto), and the prevalence of non-ejection systolic clicks and late systolic murmurs was determined. One or both of these auscultatory findings were detected in 168 children, yielding a prevalence rate of 13-99 per 1000 in the school population. A female preponderance of 1-9:1 was present and there was a strong linear increase in prevalence with age, with a peak rate of 29-41 per 1000 in 17-year-old children. A non-ejection click was the only abnormal auscultatory finding in 123 children (73%) and a mitral systolic murmur in 8 (5%), whereas in 37 (22%) both these findings were present. Of the latter 37 children, the murmur was late systolic in 32; in 5 it was early systolic. Auscultation in different postures was important in the detection of both non-ejection clicks and mitral systolic murmurs. Experience in the detection of these auscultatory findings influenced the frequency with which they were heard. Electrocardiographic abnormalities compatible with those previously described in the billowing mitral leaflet syndrome were present in 11 of 158 children. The aetiology of these auscultatory findings in this community remains unknown. In the same survey, a high prevalence rate of rheumatic heart disease was recorded and the epidemiology of the non-ejection clicks and these mitral systolic murmurs showed similarties to that of rheumatic heart disease. Though the specific billowing mitral leaflet syndrome almost certainly accounts for some of these auscultatory findings, a significant proportion may have early rheumatic heart disease. Further elucidation of this problem is necessary.", "PMID": 973897}
{"id": "pubmed23n0032_9574", "title": "An echocardiographic study of the interventricular septum in constrictive pericarditis.", "content": "Ten patients with constrictive pericarditis were studied echocardiographically with specific reference to inter-ventricular septal dynamics. Abnormal movement of the interventricular septum was present in 8 patients and consisted of flattening in systole and unusual posterior motion in diastole. The aetiology of this type of movement is at present unknown but may be related to restriction of normal cardiac rotational dynamics. The interventricular septum also showed diminished degree of thickening (mean 21-2%). The amplitude of excursion was generally at the upper limit of or greater than normal. Left ventricular posterior wall amplitude of excursion was normal. Flattening of left ventricular posterior wall diastolic movement was seen in 4 patients. Right ventricular end-diastolic dimension was slightly increased (1-2 to 1-7 cm/m2) in 5 of 8 patients with abnormal septal motion, but no haemodynamic evidence of diastolic volume overload was found. Posterior pericardial thickening was noted echocardiographically when posterior calcification was present. We conclude that the most common though non-specific feature of the echocardiogram in patients with constrictive pericarditis is abnormal septal motion. Flattening of left ventricular posterior wall diastolic movement, posterior pericardial thickening, and epicardial-pericardial separation may also occur.", "contents": "An echocardiographic study of the interventricular septum in constrictive pericarditis. Ten patients with constrictive pericarditis were studied echocardiographically with specific reference to inter-ventricular septal dynamics. Abnormal movement of the interventricular septum was present in 8 patients and consisted of flattening in systole and unusual posterior motion in diastole. The aetiology of this type of movement is at present unknown but may be related to restriction of normal cardiac rotational dynamics. The interventricular septum also showed diminished degree of thickening (mean 21-2%). The amplitude of excursion was generally at the upper limit of or greater than normal. Left ventricular posterior wall amplitude of excursion was normal. Flattening of left ventricular posterior wall diastolic movement was seen in 4 patients. Right ventricular end-diastolic dimension was slightly increased (1-2 to 1-7 cm/m2) in 5 of 8 patients with abnormal septal motion, but no haemodynamic evidence of diastolic volume overload was found. Posterior pericardial thickening was noted echocardiographically when posterior calcification was present. We conclude that the most common though non-specific feature of the echocardiogram in patients with constrictive pericarditis is abnormal septal motion. Flattening of left ventricular posterior wall diastolic movement, posterior pericardial thickening, and epicardial-pericardial separation may also occur.", "PMID": 973898}
{"id": "pubmed23n0032_9575", "title": "Clinical experience with technetium-99m stannous polyphosphate for myocardial imaging.", "content": "Myocardial imaging with technetium-99m stannous polyphosphate was performed on 46 patients. Eleven patients had no cardiac disease, 22 had acute myocardial infarction, and 13 had stable arteriosclerotic heart disease. Distinct patterns of myocardial activity were noted: (1) the patients with no obvious cardiac disease showed no cardiac activity; (2) stable arteriosclerotic heart disease showed faint, ill-defined cardiac activity, primarily in the anterior or inferior aspect of the left ventricle; (3) acute myocardial infarction showed intense, focal, well-defined activity, with a shape that characterized the location of the infarct.", "contents": "Clinical experience with technetium-99m stannous polyphosphate for myocardial imaging. Myocardial imaging with technetium-99m stannous polyphosphate was performed on 46 patients. Eleven patients had no cardiac disease, 22 had acute myocardial infarction, and 13 had stable arteriosclerotic heart disease. Distinct patterns of myocardial activity were noted: (1) the patients with no obvious cardiac disease showed no cardiac activity; (2) stable arteriosclerotic heart disease showed faint, ill-defined cardiac activity, primarily in the anterior or inferior aspect of the left ventricle; (3) acute myocardial infarction showed intense, focal, well-defined activity, with a shape that characterized the location of the infarct.", "PMID": 973899}
{"id": "pubmed23n0032_9576", "title": "Anxiety after a heart attack.", "content": "An objective measurement of anxiety at defined intervals after the onset of acute cardiac symptoms was made in 203 men admitted to the Coronary Care Unit, Royal Infirmary of Edinburgh, and in 83 patients in a Teesside coronary survey. Of the Teesside patients, 50 were treated at home, 22 were admitted initially to a coronary care unit, and 11 were admitted directly to a general medical ward. In the Edinburgh patients the level of anxiety was high early in the illness, fell rapidly, and rose again towards the end of their stay in hospital. At 4 months it was that of a normal population. After transfer from the coronary care unit the group was not more anxious than other patients in the ward. Reaction to the illness was unrelated to its physical severity. Patients who reacted badly at the beginning were less likely to return to work. The pattern of anxiety in the Teesside patients resembled that of the Edinburgh group, and reaction to illness was largely independent of physical aspects. Treatment in hospital, either through a coronary care unit initially or in a medical ward, did not increase emotional distress. At 3 months patients treated initially in a coronary care unit were less anxious than the others. Throughout the period of study the Teesside patients were more anxious than the Edinburgh patients and outcome was not related to anxiety. Social and environmental differences may account for this.", "contents": "Anxiety after a heart attack. An objective measurement of anxiety at defined intervals after the onset of acute cardiac symptoms was made in 203 men admitted to the Coronary Care Unit, Royal Infirmary of Edinburgh, and in 83 patients in a Teesside coronary survey. Of the Teesside patients, 50 were treated at home, 22 were admitted initially to a coronary care unit, and 11 were admitted directly to a general medical ward. In the Edinburgh patients the level of anxiety was high early in the illness, fell rapidly, and rose again towards the end of their stay in hospital. At 4 months it was that of a normal population. After transfer from the coronary care unit the group was not more anxious than other patients in the ward. Reaction to the illness was unrelated to its physical severity. Patients who reacted badly at the beginning were less likely to return to work. The pattern of anxiety in the Teesside patients resembled that of the Edinburgh group, and reaction to illness was largely independent of physical aspects. Treatment in hospital, either through a coronary care unit initially or in a medical ward, did not increase emotional distress. At 3 months patients treated initially in a coronary care unit were less anxious than the others. Throughout the period of study the Teesside patients were more anxious than the Edinburgh patients and outcome was not related to anxiety. Social and environmental differences may account for this.", "PMID": 973900}
{"id": "pubmed23n0032_9577", "title": "Intractable paroxysmal tachycardia caused by a concealed retrogradely conducting Kent bundle. Demonstration by epicardial mapping and cure of tachycardias by surgical interruption of the His bundle.", "content": "Electrophysiological and epicardial mapping studies are described in a patient without pre-excitation who had intractable recurrent paroxysmal supraventricular tachycardia. Electrophysiological studies revealed fixed VA conduction times during both rapid ventricular pacing and coupled ventricular stimulation. Catheter mapping of atrial activation during retrograde conduction and during induced paroxysmal supraventricular tachycardia revealed early distal coronary sinus activation (posterior left atrium) relative to the low septal, low lateral, and high lateral right atrium. These studies suggested the presence of a concealed left-sided bypass tract. The patient underwent surgical interruption of the His bundle for control of paroxysmal supraventricular tachycardia. Epicardial mapping of the atria (during ventricular pacing) confirmed the presence of a concealed left-sided bypass tract. Surgery produced antegrade av block (while retrograde conduction was maintained) and total cure of paroxysmal supraventricular tachycardia. This is the first reported case of a concealed retrograde extranodal pathway documented by epicardial mapping.", "contents": "Intractable paroxysmal tachycardia caused by a concealed retrogradely conducting Kent bundle. Demonstration by epicardial mapping and cure of tachycardias by surgical interruption of the His bundle. Electrophysiological and epicardial mapping studies are described in a patient without pre-excitation who had intractable recurrent paroxysmal supraventricular tachycardia. Electrophysiological studies revealed fixed VA conduction times during both rapid ventricular pacing and coupled ventricular stimulation. Catheter mapping of atrial activation during retrograde conduction and during induced paroxysmal supraventricular tachycardia revealed early distal coronary sinus activation (posterior left atrium) relative to the low septal, low lateral, and high lateral right atrium. These studies suggested the presence of a concealed left-sided bypass tract. The patient underwent surgical interruption of the His bundle for control of paroxysmal supraventricular tachycardia. Epicardial mapping of the atria (during ventricular pacing) confirmed the presence of a concealed left-sided bypass tract. Surgery produced antegrade av block (while retrograde conduction was maintained) and total cure of paroxysmal supraventricular tachycardia. This is the first reported case of a concealed retrograde extranodal pathway documented by epicardial mapping.", "PMID": 973901}
{"id": "pubmed23n0032_9578", "title": "Angiographic demonstration of blood supply of right atrial myxoma.", "content": "A 53-year-old woman underwent cardiac catheterization for assessment of coronary arterial disease. An unexpected finding of a gradient between right atrium and right ventricle led to the discovery of an unsuspected right atrial myxoma. The diagnosis was established by the presence of a filling defect in the right atrial angiocardiogram and by the demonstration of \"tumour vessels\" by selective right coronary angiography. Selective coronary angiography can visualize the blood supply to an intracardiac tumour and thus confirm the diagnosis preoperatively.", "contents": "Angiographic demonstration of blood supply of right atrial myxoma. A 53-year-old woman underwent cardiac catheterization for assessment of coronary arterial disease. An unexpected finding of a gradient between right atrium and right ventricle led to the discovery of an unsuspected right atrial myxoma. The diagnosis was established by the presence of a filling defect in the right atrial angiocardiogram and by the demonstration of \"tumour vessels\" by selective right coronary angiography. Selective coronary angiography can visualize the blood supply to an intracardiac tumour and thus confirm the diagnosis preoperatively.", "PMID": 973902}
{"id": "pubmed23n0032_9579", "title": "Electrical alternans of TU wave in Romano-Ward syndrome.", "content": "A case is presented in which an exceptional electrical alternans of the TU wave occurred in association with the Romano-Ward syndrome. This appears to be the first reported example of this association. Ventricular fibrillation was documented during the syncopal attacks. There was no evidence of abnormality of the serum electrolytes.", "contents": "Electrical alternans of TU wave in Romano-Ward syndrome. A case is presented in which an exceptional electrical alternans of the TU wave occurred in association with the Romano-Ward syndrome. This appears to be the first reported example of this association. Ventricular fibrillation was documented during the syncopal attacks. There was no evidence of abnormality of the serum electrolytes.", "PMID": 973903}
{"id": "pubmed23n0032_9580", "title": "Diazepam in acute myocardial infarction. Clinical effects and effects on catecholamines, free fatty acids, and cortisol.", "content": "Diazepam is a valuable drug in cases of acute myocardial infarction. The 10 mg intravenous loading dose and the subsequent 15 mg oral dose of diazepam administered three times daily produced safe, pleasant sedation, and reduced the need for analgesics. A much reduced excretion of catecholamines was recorded. It is presumed that diazepam causes a lower stress reaction, which is beneficial in diminishing the incidence of malignant arrhythmias and preventing the existing myocardial injury from spreading.", "contents": "Diazepam in acute myocardial infarction. Clinical effects and effects on catecholamines, free fatty acids, and cortisol. Diazepam is a valuable drug in cases of acute myocardial infarction. The 10 mg intravenous loading dose and the subsequent 15 mg oral dose of diazepam administered three times daily produced safe, pleasant sedation, and reduced the need for analgesics. A much reduced excretion of catecholamines was recorded. It is presumed that diazepam causes a lower stress reaction, which is beneficial in diminishing the incidence of malignant arrhythmias and preventing the existing myocardial injury from spreading.", "PMID": 973905}
{"id": "pubmed23n0032_9581", "title": "Calcific coronary embolization associated with cardiac valve replacement. Necropsy x-ray study.", "content": "The hearts of 14 patients dying early after replacement of a calcified aortic or mitral valve were examined for the presence of calcific coronary emboli. The incidence of embolization as shown by x-ray film was 92-9 per cent. The number of emboli in individual cases ranged from 4 to 119. Replacement of an aortic valve was associated with a more severe embolization than replacement of a mitral valve. Th mechanisms of calcific embolization are discussed and preventive surgical measures suggested.", "contents": "Calcific coronary embolization associated with cardiac valve replacement. Necropsy x-ray study. The hearts of 14 patients dying early after replacement of a calcified aortic or mitral valve were examined for the presence of calcific coronary emboli. The incidence of embolization as shown by x-ray film was 92-9 per cent. The number of emboli in individual cases ranged from 4 to 119. Replacement of an aortic valve was associated with a more severe embolization than replacement of a mitral valve. Th mechanisms of calcific embolization are discussed and preventive surgical measures suggested.", "PMID": 973906}
{"id": "pubmed23n0032_9582", "title": "Pancarditis affecting the conducting system and all valves in human African trypanosomiasis.", "content": "This paper describes the pathological features of 10 cases of human African trypanosomiasis occurring in Uganda over an 8-year period. Three cases were clinically proved, and in all 10 cases chronic meningoencephalitis and myocarditis were present. There was chronic pancarditis in 6 cases, and in 3 of these there was a generalized focal valvulitis and in 2 lesions of the conducting system. Previous descriptions of carditis in human African trypanosomiasis are briefly reviewed, and the present findings are compared with some cardiomyopathies as well as American human and experimental trypanosomiasis.", "contents": "Pancarditis affecting the conducting system and all valves in human African trypanosomiasis. This paper describes the pathological features of 10 cases of human African trypanosomiasis occurring in Uganda over an 8-year period. Three cases were clinically proved, and in all 10 cases chronic meningoencephalitis and myocarditis were present. There was chronic pancarditis in 6 cases, and in 3 of these there was a generalized focal valvulitis and in 2 lesions of the conducting system. Previous descriptions of carditis in human African trypanosomiasis are briefly reviewed, and the present findings are compared with some cardiomyopathies as well as American human and experimental trypanosomiasis.", "PMID": 973907}
{"id": "pubmed23n0032_9583", "title": "Mobitz II AV block within the His bundle, with progression to complete heart block.", "content": "A case of a 48-year-old woman with frequent syncopal episodes is reported. The electrocardiogram showed high degree AV block with narrow QRS complexes. The His bundle electrogram displayed a split His deflection indicating impairment of conduction within the His bundle of the Mobitz II type. The AH interval was prolonged and Wenckebach phenomenon occurred at the same atrial pacing rate before and after atropine administration. During spontaneous or induced high grade AV block an escape rhythm originating in the distal His bundle was observed. A secondary study performed one year later showed progression to complete AV block. Both His potentials were present, one following the atrial and the other preceding the ventricular deflection. The H'V interval was prolonged and a further lengthening was seen after ajmaline. All these findings indicated proximal, mid, and distal disease of the His trunk.", "contents": "Mobitz II AV block within the His bundle, with progression to complete heart block. A case of a 48-year-old woman with frequent syncopal episodes is reported. The electrocardiogram showed high degree AV block with narrow QRS complexes. The His bundle electrogram displayed a split His deflection indicating impairment of conduction within the His bundle of the Mobitz II type. The AH interval was prolonged and Wenckebach phenomenon occurred at the same atrial pacing rate before and after atropine administration. During spontaneous or induced high grade AV block an escape rhythm originating in the distal His bundle was observed. A secondary study performed one year later showed progression to complete AV block. Both His potentials were present, one following the atrial and the other preceding the ventricular deflection. The H'V interval was prolonged and a further lengthening was seen after ajmaline. All these findings indicated proximal, mid, and distal disease of the His trunk.", "PMID": 973908}
{"id": "pubmed23n0032_9584", "title": "The pharmacokinetics of ephedrine after oral dosage in asthmatics receiving acute and chronic treatment.", "content": "1 Ephedrine plasma levels have been measured in ten asthmatic patients given a singel dose of ephedrine hydrochloride (22 mg) along and in combination with theophylline and a barbiturate. 2 Pharmacokinetic assessment of the data indicated no significant intra-subject changes in kinetic parameters before or after chronic treatment with ephedrine HCl (11 mg three times a day) alone or in combination. 3 Tolerance to these therapeutic doses, if it occurs, is therefore not disposition-related but rather to pharmacodynamic changes.", "contents": "The pharmacokinetics of ephedrine after oral dosage in asthmatics receiving acute and chronic treatment. 1 Ephedrine plasma levels have been measured in ten asthmatic patients given a singel dose of ephedrine hydrochloride (22 mg) along and in combination with theophylline and a barbiturate. 2 Pharmacokinetic assessment of the data indicated no significant intra-subject changes in kinetic parameters before or after chronic treatment with ephedrine HCl (11 mg three times a day) alone or in combination. 3 Tolerance to these therapeutic doses, if it occurs, is therefore not disposition-related but rather to pharmacodynamic changes.", "PMID": 973934}
{"id": "pubmed23n0032_9585", "title": "A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry.", "content": "1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.", "contents": "A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry. 1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.", "PMID": 973935}
{"id": "pubmed23n0032_9586", "title": "Simultaneous quantitative gas-chromatographic analysis of ethosuximide, phenobarbitone, primidone and diphenylhydantoin.", "content": "1 Therapeutic serum concentrations of ethosuximide, phenobarbitone, primidone, and dipheylhydantoin were assayed from 1 ml of human serum. The extraction procedure was common to all four drugs and three internal standards. 2 Subsequent isothermal gas chromatographic analysis of serum extracts produced well resolved peaks for the underivatized quantitation of ethosuximide and phenobarbitone. Primidone and diphenylhydantoin were determined as methylated derivatives. 3 Mean coefficients of variation for the assay of each drug were less 7% on a newly packed and conditioned column and less than 10% after the technique had been in continuous use for 3 months. 4 The advantage of quantitation relative to peak area ratios rather than peak height ratios was minimal for the determination of ethosuximide, primidone and diphenylhydantoin but appeared significant for the assay of phenobarbitone.", "contents": "Simultaneous quantitative gas-chromatographic analysis of ethosuximide, phenobarbitone, primidone and diphenylhydantoin. 1 Therapeutic serum concentrations of ethosuximide, phenobarbitone, primidone, and dipheylhydantoin were assayed from 1 ml of human serum. The extraction procedure was common to all four drugs and three internal standards. 2 Subsequent isothermal gas chromatographic analysis of serum extracts produced well resolved peaks for the underivatized quantitation of ethosuximide and phenobarbitone. Primidone and diphenylhydantoin were determined as methylated derivatives. 3 Mean coefficients of variation for the assay of each drug were less 7% on a newly packed and conditioned column and less than 10% after the technique had been in continuous use for 3 months. 4 The advantage of quantitation relative to peak area ratios rather than peak height ratios was minimal for the determination of ethosuximide, primidone and diphenylhydantoin but appeared significant for the assay of phenobarbitone.", "PMID": 973936}
{"id": "pubmed23n0032_9587", "title": "Renal function, protein binding and pharmacological response to diazoxide.", "content": "1 The effect of rapid (10s) injections of diazoxide was studied in ten hypertensive patients with varying degrees of impairment of renal function. 2 There was a significant correlation between the patient's plasma urea concentration and reduction in mean arterial blood pressure. Diazoxide was also shown to be less highly protein bound in patients with renal failure. 3 It is suggested that the explanation for the increased hypotensive effect of diazoxide observed in patients with reduced renal function is related to higher unbound drug concentrations.", "contents": "Renal function, protein binding and pharmacological response to diazoxide. 1 The effect of rapid (10s) injections of diazoxide was studied in ten hypertensive patients with varying degrees of impairment of renal function. 2 There was a significant correlation between the patient's plasma urea concentration and reduction in mean arterial blood pressure. Diazoxide was also shown to be less highly protein bound in patients with renal failure. 3 It is suggested that the explanation for the increased hypotensive effect of diazoxide observed in patients with reduced renal function is related to higher unbound drug concentrations.", "PMID": 973937}
{"id": "pubmed23n0032_9588", "title": "Comparative effects of acebutolol and practolol on the lipolytic response to isoprenaline.", "content": "1 The effects of beta-adrenoceptor blockade on the metabolic responses to isoprenaline have been studied in an in vitro system of isolated fat cells and in six normal subjects. 2 The inhibitory effects of varying concentrations of acebutolol, practolol and propranolol on free fatty acid (FFA) release produced by isoprenaline (10(-7) M) were compared in isolated fat cells prepared from rat epididymal adipose tissue. Acebutolol and practolol, at equimolar concentrations, showed a similar inhibitory effect whilst propranolol was approximately 100 times more potent then either drug. At 10(-5)M concentration of propranolol, lipolysis was virtually abolished whilst at the same molar concentration, acebutolol and practolol halved the response. 3 Six healthy volunteers received three successive 15 min intravenous isoprenaline challenges (0.03 mug kg-1 min-1) per individual experiment. The first acted as a control whilst the following two were given either after single oral doses of placebo, acebutolol or practolol. The mean (+/- s.e. mean) basal FFA level was 0.77 +/- 0.06 mE1/1 and subsequent resting values after the administration of placebo or beta-adrenoceptor blocker were not significantly different. 4 Acebutolol inhibited the respective mean rises in FFA, produced by both post-control isoprenaline challenges, by (mean +/- s.e. mean) 70 +/- 4% and 84% +/- 5%. The comparable figures for practolol were 33 +/- 15% and 24 +/- 20%. The higher serum concentration of acebutolol produced greater inhibition but correlation of log serum concentration of the drug with percentage inhibition of FFA rise did not achieve significance. 5 Administration of isoprenaline, acebutolol or practolol did not significantly alter serum glucose, triglyceride or cholesterol levels. 6 Acebutolol and practolol effectively blocked the isoprenaline-induced tachycardia. The degree of blockade produced by practolol was greater than its inhibitory effect on FFA release. The diatolic fall in blood pressure in response to isoprenaline was abolished by acebutolol suggesting that its beta-adrenoceptor blocking action encompasses peripheral vascular sites. The comparable effect with practolol was a partial inhibition of the diastolic fall.", "contents": "Comparative effects of acebutolol and practolol on the lipolytic response to isoprenaline. 1 The effects of beta-adrenoceptor blockade on the metabolic responses to isoprenaline have been studied in an in vitro system of isolated fat cells and in six normal subjects. 2 The inhibitory effects of varying concentrations of acebutolol, practolol and propranolol on free fatty acid (FFA) release produced by isoprenaline (10(-7) M) were compared in isolated fat cells prepared from rat epididymal adipose tissue. Acebutolol and practolol, at equimolar concentrations, showed a similar inhibitory effect whilst propranolol was approximately 100 times more potent then either drug. At 10(-5)M concentration of propranolol, lipolysis was virtually abolished whilst at the same molar concentration, acebutolol and practolol halved the response. 3 Six healthy volunteers received three successive 15 min intravenous isoprenaline challenges (0.03 mug kg-1 min-1) per individual experiment. The first acted as a control whilst the following two were given either after single oral doses of placebo, acebutolol or practolol. The mean (+/- s.e. mean) basal FFA level was 0.77 +/- 0.06 mE1/1 and subsequent resting values after the administration of placebo or beta-adrenoceptor blocker were not significantly different. 4 Acebutolol inhibited the respective mean rises in FFA, produced by both post-control isoprenaline challenges, by (mean +/- s.e. mean) 70 +/- 4% and 84% +/- 5%. The comparable figures for practolol were 33 +/- 15% and 24 +/- 20%. The higher serum concentration of acebutolol produced greater inhibition but correlation of log serum concentration of the drug with percentage inhibition of FFA rise did not achieve significance. 5 Administration of isoprenaline, acebutolol or practolol did not significantly alter serum glucose, triglyceride or cholesterol levels. 6 Acebutolol and practolol effectively blocked the isoprenaline-induced tachycardia. The degree of blockade produced by practolol was greater than its inhibitory effect on FFA release. The diatolic fall in blood pressure in response to isoprenaline was abolished by acebutolol suggesting that its beta-adrenoceptor blocking action encompasses peripheral vascular sites. The comparable effect with practolol was a partial inhibition of the diastolic fall.", "PMID": 973938}
{"id": "pubmed23n0032_9589", "title": "Human pharmacokinetic and pharmacodynamic studies on the atenolo (ICI 66,082), a new cardioselective beta-adrenoceptor blocking drug.", "content": "The beta-adrenoceptor blocking effects of orally administered atenolol on tachycardia induced by intravenous isoprenaline or by exercise have been studied in normal volunteers, and compared with the effects of similar doses of propranolol. The blood levels of atenolol at various times after oral administration were determined by g.l.c. and correlated with the degree of inhibition of tachycardia. Atenolol was shown to be a beta-adrenoceptor blocker in man, as in animals, in that it antagonized the chronotropic effects of isoprenaline and of exercise. The inhibitory effect of atenolol on exercise-induced tachycardia was evident at a concentration in blood of 0.2 mug/ml and virtually complete at 0.5 mug/ml. Higher concentrations than this did not produce significantly greater blockade. The effects of atenolol on exercise-induced tachycardia were similar to those of propranolol but it was less effective in blocking the rise in heart rate and fall in diastolic blood-pressure induced by intravenous infusion of isoprenaline. This separation of effects is considered characteristic of drugs causing preferential blockade of cardiac beta-adrenoreceptors. The half-life of atenolol in blood was calculated to ablut 9 hours.", "contents": "Human pharmacokinetic and pharmacodynamic studies on the atenolo (ICI 66,082), a new cardioselective beta-adrenoceptor blocking drug. The beta-adrenoceptor blocking effects of orally administered atenolol on tachycardia induced by intravenous isoprenaline or by exercise have been studied in normal volunteers, and compared with the effects of similar doses of propranolol. The blood levels of atenolol at various times after oral administration were determined by g.l.c. and correlated with the degree of inhibition of tachycardia. Atenolol was shown to be a beta-adrenoceptor blocker in man, as in animals, in that it antagonized the chronotropic effects of isoprenaline and of exercise. The inhibitory effect of atenolol on exercise-induced tachycardia was evident at a concentration in blood of 0.2 mug/ml and virtually complete at 0.5 mug/ml. Higher concentrations than this did not produce significantly greater blockade. The effects of atenolol on exercise-induced tachycardia were similar to those of propranolol but it was less effective in blocking the rise in heart rate and fall in diastolic blood-pressure induced by intravenous infusion of isoprenaline. This separation of effects is considered characteristic of drugs causing preferential blockade of cardiac beta-adrenoreceptors. The half-life of atenolol in blood was calculated to ablut 9 hours.", "PMID": 973955}
{"id": "pubmed23n0032_9590", "title": "Digoxin pharmacokinetics: multicompartmental analysis and its clinical implications.", "content": "The kinetics of digoxin have been investigated in healthy volunteers using an isotopic tracer technique. A three compartment open kinetic model has been proposed as the simplest model consistent with the plasma, urinary and faecal data obtained. The renal clearance of digoxin (mean +/- s.d.) was found to be 119+/-10 ml/min, which did not differ significantly from the glomerular filtration rate (110+/-14 ml/min). Digoxin extra-renal clearance (mean+/-s.d.) was found to be 47+/-7 ml/min. The model predicts that the tissue concentration attained after four 0.25 mg oral doses spread over 24 h can be achieved within a period of 4 h following a single oral loading dose of 1 mg. Maintenance doses can be derived from a simple formula based on the glomerular filtration rate, extra-renal clearance and bioavailability of the digoxin preparation used.", "contents": "Digoxin pharmacokinetics: multicompartmental analysis and its clinical implications. The kinetics of digoxin have been investigated in healthy volunteers using an isotopic tracer technique. A three compartment open kinetic model has been proposed as the simplest model consistent with the plasma, urinary and faecal data obtained. The renal clearance of digoxin (mean +/- s.d.) was found to be 119+/-10 ml/min, which did not differ significantly from the glomerular filtration rate (110+/-14 ml/min). Digoxin extra-renal clearance (mean+/-s.d.) was found to be 47+/-7 ml/min. The model predicts that the tissue concentration attained after four 0.25 mg oral doses spread over 24 h can be achieved within a period of 4 h following a single oral loading dose of 1 mg. Maintenance doses can be derived from a simple formula based on the glomerular filtration rate, extra-renal clearance and bioavailability of the digoxin preparation used.", "PMID": 973956}
{"id": "pubmed23n0032_9591", "title": "Can digoxin dose requirements be predicted?", "content": "A search for patient variables relevant to digoxin dose requirements was made in fourty-three patients with a wide range of renal and hepatic function. The daily dose of digoxin to achieve a mean serum concentration of 1.5 ng/ml, the standardized dose, was calculated for each patient. The standardized dose correlated significantly with the following variables, in descending order of correlation coefficient; creatinine clearance, serum creatinine concentration, body weight and serum albumin concentration. An equation containing the two independent variables, creatinine clearance and serum albumin concentration, had a significantly stronger correlation with standardized dose than creatinine clearance alone. Attempts were made in each patient to predict the standardized dose using both empirical prescribing methods and the published nomograms. Although a maximum of 70% of the variance of the standardized dose was explained, this corresponded approximately to one patient in three having a predicted dose outside the 95% confidnece limits for the standardized dose. There remain important sources of individual variation in digoxin dose requirements yet to be identified. Future application of empirical prescribing methods, such as multiple linear regression and Bayes' theorem, to prescription for large, defined patient groups may improve dose prediction for individual patients.", "contents": "Can digoxin dose requirements be predicted? A search for patient variables relevant to digoxin dose requirements was made in fourty-three patients with a wide range of renal and hepatic function. The daily dose of digoxin to achieve a mean serum concentration of 1.5 ng/ml, the standardized dose, was calculated for each patient. The standardized dose correlated significantly with the following variables, in descending order of correlation coefficient; creatinine clearance, serum creatinine concentration, body weight and serum albumin concentration. An equation containing the two independent variables, creatinine clearance and serum albumin concentration, had a significantly stronger correlation with standardized dose than creatinine clearance alone. Attempts were made in each patient to predict the standardized dose using both empirical prescribing methods and the published nomograms. Although a maximum of 70% of the variance of the standardized dose was explained, this corresponded approximately to one patient in three having a predicted dose outside the 95% confidnece limits for the standardized dose. There remain important sources of individual variation in digoxin dose requirements yet to be identified. Future application of empirical prescribing methods, such as multiple linear regression and Bayes' theorem, to prescription for large, defined patient groups may improve dose prediction for individual patients.", "PMID": 973957}
{"id": "pubmed23n0032_9592", "title": "A study of factors influencing drug disposition in chronic liver disease, using the model drug (+)-propranolol.", "content": "The pharmacokinetics, following i.v. administration of (+)-propranolol (40 mg) have been compared to in vitro measurement of protein binding and biochemical parameters of liver function in six normal subjects and twenty patients with stable chronic liver disease. The clearance of (+)-propranolol decreased with evidence of increasing severity of impairment of liver function correlating significantly with a fall in serum albumin, a rise in bilirubin and a prolongation in prothrombin index. The clearance of (+)-propranolol correlated with and was numerically similar to the clearance of indocyanine green in normal subjects and also in patients with chronic liver disease. Protein binding was decreased in chronic liver disease, but this change was not related to changes in plasma proteins. In normal subjects and patients without ascites the volume of distribution increased with decreases in protein binding. Ascites was associated with a further increase in the volume of distribution. The considerable variation in half-life largely depends on changes in liver blood flow, the degree of protein binding and the plasma protein pool size.", "contents": "A study of factors influencing drug disposition in chronic liver disease, using the model drug (+)-propranolol. The pharmacokinetics, following i.v. administration of (+)-propranolol (40 mg) have been compared to in vitro measurement of protein binding and biochemical parameters of liver function in six normal subjects and twenty patients with stable chronic liver disease. The clearance of (+)-propranolol decreased with evidence of increasing severity of impairment of liver function correlating significantly with a fall in serum albumin, a rise in bilirubin and a prolongation in prothrombin index. The clearance of (+)-propranolol correlated with and was numerically similar to the clearance of indocyanine green in normal subjects and also in patients with chronic liver disease. Protein binding was decreased in chronic liver disease, but this change was not related to changes in plasma proteins. In normal subjects and patients without ascites the volume of distribution increased with decreases in protein binding. Ascites was associated with a further increase in the volume of distribution. The considerable variation in half-life largely depends on changes in liver blood flow, the degree of protein binding and the plasma protein pool size.", "PMID": 973958}
{"id": "pubmed23n0032_9593", "title": "beta-Adrenoceptor blocking activity and duration of action of pindolol and propranolol in healthy volunteers.", "content": "The beta-adrenoceptor blocking activities of pindolol and propranolol have been investigated in healthy male volunteers. Pindolol was about forty times more potent than propranolol in reducing isoprenaline-induced tachycardia. Pindolol (5 mg) and propranolol (u99 mg) were approximately equiactive in reducing exercise-induced tachycardia, 2 h after oral administration. The duration of action of pindolol is significantly longer than that of propranolol; 24 h after pindolol (kmg), 36+/-5% of the masimum effect were still present, and after propranolol (100 mg) 16+/-4% remained. Despite the long duration of action of pindolol, there was no evidence for cumulation during oral administration of 5 mg t.d.s. for 5 days.", "contents": "beta-Adrenoceptor blocking activity and duration of action of pindolol and propranolol in healthy volunteers. The beta-adrenoceptor blocking activities of pindolol and propranolol have been investigated in healthy male volunteers. Pindolol was about forty times more potent than propranolol in reducing isoprenaline-induced tachycardia. Pindolol (5 mg) and propranolol (u99 mg) were approximately equiactive in reducing exercise-induced tachycardia, 2 h after oral administration. The duration of action of pindolol is significantly longer than that of propranolol; 24 h after pindolol (kmg), 36+/-5% of the masimum effect were still present, and after propranolol (100 mg) 16+/-4% remained. Despite the long duration of action of pindolol, there was no evidence for cumulation during oral administration of 5 mg t.d.s. for 5 days.", "PMID": 973959}
{"id": "pubmed23n0032_9594", "title": "The effect of end-stage renal failure and haemodialysis on the elimination kinetics of sotalol.", "content": "A single oral dose of sotalol (160 mg) was administered to control subjects with normal renal function and patients with chronic renal failure in the interdialysis period to estimate the elimination kinetics of the drug. Sotalol concentrations in body fluids were measured fluorimetrically using a modified Garrett and Schnelle (1971) method. Mean plasma half-life (T 1/2) was approximately 5 h in normals, 42 h in patients off-dialysis. During haemodialysis the mean plasma half-time was on the average 7 hours. Comulative urinary excretion of the drug was considerably lower in the patient group: 9% of the dose in 48 h as opposed to 61% in normals. Comparison of sotalol concentrations in plasma versus ultrafiltrate from the coil kidney indicates that the drug in vivo is negligible bound to plasma proteins in remal patients. The net-lowering effect of a 6 to 7 h haemodialysis on the plasma concentration decay line was by 20%. Post-dialysis plasma concentration data suggest that the rate at which sotalol returns to plasma from body tissues appears to be the rate-controlling factor in the elimination of sotalol by haemodialysis.", "contents": "The effect of end-stage renal failure and haemodialysis on the elimination kinetics of sotalol. A single oral dose of sotalol (160 mg) was administered to control subjects with normal renal function and patients with chronic renal failure in the interdialysis period to estimate the elimination kinetics of the drug. Sotalol concentrations in body fluids were measured fluorimetrically using a modified Garrett and Schnelle (1971) method. Mean plasma half-life (T 1/2) was approximately 5 h in normals, 42 h in patients off-dialysis. During haemodialysis the mean plasma half-time was on the average 7 hours. Comulative urinary excretion of the drug was considerably lower in the patient group: 9% of the dose in 48 h as opposed to 61% in normals. Comparison of sotalol concentrations in plasma versus ultrafiltrate from the coil kidney indicates that the drug in vivo is negligible bound to plasma proteins in remal patients. The net-lowering effect of a 6 to 7 h haemodialysis on the plasma concentration decay line was by 20%. Post-dialysis plasma concentration data suggest that the rate at which sotalol returns to plasma from body tissues appears to be the rate-controlling factor in the elimination of sotalol by haemodialysis.", "PMID": 973960}
{"id": "pubmed23n0032_9595", "title": "The influence of smoking on the intersubject variation in pentazocine elimination.", "content": "The cumulative urinary excretion over 24 h of pentazocine, under conditions of acidic urinary pH, has been measured in smokers and non-smokers using both male and female subjects (seventy subjects in total). A restricted urban population was studied. An overall three-fold inter-subject variation in elimination was observed. The cumulative urinary excretion of pentazocine was normally distributed in both smokers and non-smokers. Smokers metabolize 40% more pentazocine than non-smokers. It is concluded that induction is principally responsible for the observed subject variability.", "contents": "The influence of smoking on the intersubject variation in pentazocine elimination. The cumulative urinary excretion over 24 h of pentazocine, under conditions of acidic urinary pH, has been measured in smokers and non-smokers using both male and female subjects (seventy subjects in total). A restricted urban population was studied. An overall three-fold inter-subject variation in elimination was observed. The cumulative urinary excretion of pentazocine was normally distributed in both smokers and non-smokers. Smokers metabolize 40% more pentazocine than non-smokers. It is concluded that induction is principally responsible for the observed subject variability.", "PMID": 973961}
{"id": "pubmed23n0032_9596", "title": "The influence of various factors in the in vitro distribution of haloperidol in human blood.", "content": "Haloperidol is 89.6+/-0.3% bound (mean+/-s.e. mean) in human plasma under in vitro conditions and the free drug distributes rapidly between the plasma and the cellular elements of blood. The cell/plasma partition ratio was 1.12+/-0.06 (mean+/-s.e. mean). Alteration of plasma binding by dilution with buffer showed that uptake of haloperidol by the cellular elements of blood was proportional to free drug concentration. Bishydroxycoumarin (95 ot 286 mug/ml) reduced plasma binding of haloperidol and the displaced haloperidol was taken up by the cellular blood elements. The experiments indicate that the cellular compartment of blood as well as the plasma compartment may act as a sink for haloperidol and drug displacement interactions should therefore be interpreted with a knowledge of both of these compartments.", "contents": "The influence of various factors in the in vitro distribution of haloperidol in human blood. Haloperidol is 89.6+/-0.3% bound (mean+/-s.e. mean) in human plasma under in vitro conditions and the free drug distributes rapidly between the plasma and the cellular elements of blood. The cell/plasma partition ratio was 1.12+/-0.06 (mean+/-s.e. mean). Alteration of plasma binding by dilution with buffer showed that uptake of haloperidol by the cellular elements of blood was proportional to free drug concentration. Bishydroxycoumarin (95 ot 286 mug/ml) reduced plasma binding of haloperidol and the displaced haloperidol was taken up by the cellular blood elements. The experiments indicate that the cellular compartment of blood as well as the plasma compartment may act as a sink for haloperidol and drug displacement interactions should therefore be interpreted with a knowledge of both of these compartments.", "PMID": 973962}
{"id": "pubmed23n0032_9597", "title": "The effects of low doses of amylobarbitone sodium and diazepam on human performance.", "content": "The effects of diazepam (2.5 and 5 mg) and amylobarbitone sodium (50 and 100 mg) on performance and subjective effects were assessed in a group of twelve healthy subjects under standardised conditions. Treatments were administered orally at weekly intervals according to a balanced design and under double-blind conditions. The tests of performance most sensitive to drug effects in these healthy subjects were either prolonged and monotonous and gave the subject no feedback on performance, or required short term memory for efficient execution. Auditory vigilance was significantly impaired (P less than 0.05) between 45 min and 1 h 45 min after all drug treatments except amylobarbitone sodium (100 mg), compared with performance after lactose. At the same time false reports were significantly increased after amylobarbitone sodium (100 mg) compared with all other active drugs but not with lactose. These effects had disappeared 4-5 h post drug. Short term memory was impaired 1h 45 min after all treatments and impairment was dose related. No significant effects occurred 5h after treatment. Simple auditory reaction time was prolonged 2 h after the highest doses of amylobarbitone sodium and diazpam, and by amylobarbitone sodium (50 mg) 5 h 15 min after treatment. At this time the effects of diazepam had worn off. Digit symbol substitution was impaired by amylobarbitone sodium (50 and 100 mg), and diazepam (5 mg) after 2 h 45 minutes. No significant changes in visual search or tapping occurred after active drugs compared with lactose. Subjective ratings indicated both mental and motor impairment 2 h 45 min after all active preparations compared with scores after lactose though significant changes followed diazepam (2.5 mg) infrequently. Both correct detections and false reports in auditory vigilance tended to fall over the 6 separate days of testing, indicating an increase in caution. Visual search, short term memory, tapping and digit symbol substitution significantly improved with time, but there was no change in reaction time. From the limited information obtained by sampling blood at 3 and 6 h, no relationship between change in performance and plasma level was found in these subjects.", "contents": "The effects of low doses of amylobarbitone sodium and diazepam on human performance. The effects of diazepam (2.5 and 5 mg) and amylobarbitone sodium (50 and 100 mg) on performance and subjective effects were assessed in a group of twelve healthy subjects under standardised conditions. Treatments were administered orally at weekly intervals according to a balanced design and under double-blind conditions. The tests of performance most sensitive to drug effects in these healthy subjects were either prolonged and monotonous and gave the subject no feedback on performance, or required short term memory for efficient execution. Auditory vigilance was significantly impaired (P less than 0.05) between 45 min and 1 h 45 min after all drug treatments except amylobarbitone sodium (100 mg), compared with performance after lactose. At the same time false reports were significantly increased after amylobarbitone sodium (100 mg) compared with all other active drugs but not with lactose. These effects had disappeared 4-5 h post drug. Short term memory was impaired 1h 45 min after all treatments and impairment was dose related. No significant effects occurred 5h after treatment. Simple auditory reaction time was prolonged 2 h after the highest doses of amylobarbitone sodium and diazpam, and by amylobarbitone sodium (50 mg) 5 h 15 min after treatment. At this time the effects of diazepam had worn off. Digit symbol substitution was impaired by amylobarbitone sodium (50 and 100 mg), and diazepam (5 mg) after 2 h 45 minutes. No significant changes in visual search or tapping occurred after active drugs compared with lactose. Subjective ratings indicated both mental and motor impairment 2 h 45 min after all active preparations compared with scores after lactose though significant changes followed diazepam (2.5 mg) infrequently. Both correct detections and false reports in auditory vigilance tended to fall over the 6 separate days of testing, indicating an increase in caution. Visual search, short term memory, tapping and digit symbol substitution significantly improved with time, but there was no change in reaction time. From the limited information obtained by sampling blood at 3 and 6 h, no relationship between change in performance and plasma level was found in these subjects.", "PMID": 973963}
{"id": "pubmed23n0032_9598", "title": "The influence of premedication, anaesthesia, age and weight on glucose uptake into human isolated skeletal muscle.", "content": "The effect of the anaesthetic procedures and of the sex, age and weight of each patient on glucose uptake and glycogen content of human skeletal muscle has been studied in vitro in the presence and absence of insulin. Statistical analysis indicated that the relationships between age and both glucose uptake and the response to insulin were significant, older patients in general having higher uptakes. The blucose uptake was highly correlated with the three obesity indices (ponderal index, body mass index and percentage of the ideal weight). The anaesthetic agents had no significant effect on glucose uptake. The choice of premedication appeared to have a small effect on the basal glucose uptake level, but as the choice of premedication was also age related and age itself was a significant factor, this effect may not be of importance. It is concluded that the age and the degree of obesity of the patients ought to be taken into account when studying samples of human muscle.", "contents": "The influence of premedication, anaesthesia, age and weight on glucose uptake into human isolated skeletal muscle. The effect of the anaesthetic procedures and of the sex, age and weight of each patient on glucose uptake and glycogen content of human skeletal muscle has been studied in vitro in the presence and absence of insulin. Statistical analysis indicated that the relationships between age and both glucose uptake and the response to insulin were significant, older patients in general having higher uptakes. The blucose uptake was highly correlated with the three obesity indices (ponderal index, body mass index and percentage of the ideal weight). The anaesthetic agents had no significant effect on glucose uptake. The choice of premedication appeared to have a small effect on the basal glucose uptake level, but as the choice of premedication was also age related and age itself was a significant factor, this effect may not be of importance. It is concluded that the age and the degree of obesity of the patients ought to be taken into account when studying samples of human muscle.", "PMID": 973964}
{"id": "pubmed23n0032_9599", "title": "Methods for collecting individual components of mixed saliva: the relevance to clinical pharmacology.", "content": "Methods are described for collecting constituents of mixed saliva, viz. parotid, submandibular, monor gland and sublingual saliva and gingival fluid. Literature is cited which showed that using these techniques, few antibiotics could be found in mixed saliva or its main components but all were detected in gingival fluid. Rifamicin and clindamycin were found in all components.", "contents": "Methods for collecting individual components of mixed saliva: the relevance to clinical pharmacology. Methods are described for collecting constituents of mixed saliva, viz. parotid, submandibular, monor gland and sublingual saliva and gingival fluid. Literature is cited which showed that using these techniques, few antibiotics could be found in mixed saliva or its main components but all were detected in gingival fluid. Rifamicin and clindamycin were found in all components.", "PMID": 973965}
{"id": "pubmed23n0032_9600", "title": "Assessment of antipyrine kinetics by measurement in saliva.", "content": "The half-life of antipyrine has been estimated from saliva samples in ten subjects by a gas chromatographic method. Half-life, apparent volume of distribution and total body clearance estimated from saliva and plasma concentrations of antipyrine are not significantly different. The concentration of antipyrine in saliva is independent of flow rate within the range expected in healthy subjects in response to mechanical and sapid stimuli. Antipyrine estimation in saliva could facilitate many areas of phaamacokinetic research limited by the difficulty of obtaining serial plasma samples.", "contents": "Assessment of antipyrine kinetics by measurement in saliva. The half-life of antipyrine has been estimated from saliva samples in ten subjects by a gas chromatographic method. Half-life, apparent volume of distribution and total body clearance estimated from saliva and plasma concentrations of antipyrine are not significantly different. The concentration of antipyrine in saliva is independent of flow rate within the range expected in healthy subjects in response to mechanical and sapid stimuli. Antipyrine estimation in saliva could facilitate many areas of phaamacokinetic research limited by the difficulty of obtaining serial plasma samples.", "PMID": 973966}
{"id": "pubmed23n0032_9601", "title": "Factors affecting drug binding in plasma of elderly patients.", "content": "The binding of salicylate, sulphadiazine and phenylbutazone to plasma proteins has been studied in young and elderly subjects. Elderly patients had significantly reduced concentrations of plasma albumin, compared with subjects under 40 years of age. Sifnificant increases in free levels of all three drugs were found in elderly patients receiving multiple drug therapy, and a correlation obtained with the number of drugs being taken. It is suggested that because of their low albumin levels, elderly patients may be more susceptible to the effects of multiple drug therapy on drug binding. The clinical implications of these observations are discussed.", "contents": "Factors affecting drug binding in plasma of elderly patients. The binding of salicylate, sulphadiazine and phenylbutazone to plasma proteins has been studied in young and elderly subjects. Elderly patients had significantly reduced concentrations of plasma albumin, compared with subjects under 40 years of age. Sifnificant increases in free levels of all three drugs were found in elderly patients receiving multiple drug therapy, and a correlation obtained with the number of drugs being taken. It is suggested that because of their low albumin levels, elderly patients may be more susceptible to the effects of multiple drug therapy on drug binding. The clinical implications of these observations are discussed.", "PMID": 973967}
{"id": "pubmed23n0032_9602", "title": "Haemodynamic long-term effects of a new beta-adrenoceptor blocking drug, atenolol (ICI 66082), in essential hypertension.", "content": "1 Thirteen men with untreated essential hypertension in WHO stage I were studied on an outpatient basis to evaluate the haemodynamic long-tern effect of a new beta-adrenoceptor blocker, atenolol. 2 Oxygen consumption, heart rate, cardiac output (Cardiogreen) and intraarterial branchial pressure were recorded at rest in a supine and sitting position and during steady state work at 300, 600 and 900 kpm/min. 3 The subjects were treated with atenolol (dose 100-200 mg/day) as the sole drug for 1 year and the haemodynamic study was repeated. 4 The blood pressure was reduced approximately 18% both at rest and during exercise, the heart rate approximately 25% and the cardiac output 16% at rest supine and 27% at rest sitting. During exercise the reductions in cardiac output were approximately 20%. The calculated total peripheral resistance was not decreased compared to pretreatment values. The mean arterial pressure-heart rate product was reduced almost 40%. 5 Apart from temporary muscular fatigue during the first weeks, no side-effects were seen. 6 Atenolol is an effective blood pressure lowering drug in mild and moderate hypertension, but the drop in blood pressure is associated with marked reduction in heart rate and cardiac output at rest as well as during exercise.", "contents": "Haemodynamic long-term effects of a new beta-adrenoceptor blocking drug, atenolol (ICI 66082), in essential hypertension. 1 Thirteen men with untreated essential hypertension in WHO stage I were studied on an outpatient basis to evaluate the haemodynamic long-tern effect of a new beta-adrenoceptor blocker, atenolol. 2 Oxygen consumption, heart rate, cardiac output (Cardiogreen) and intraarterial branchial pressure were recorded at rest in a supine and sitting position and during steady state work at 300, 600 and 900 kpm/min. 3 The subjects were treated with atenolol (dose 100-200 mg/day) as the sole drug for 1 year and the haemodynamic study was repeated. 4 The blood pressure was reduced approximately 18% both at rest and during exercise, the heart rate approximately 25% and the cardiac output 16% at rest supine and 27% at rest sitting. During exercise the reductions in cardiac output were approximately 20%. The calculated total peripheral resistance was not decreased compared to pretreatment values. The mean arterial pressure-heart rate product was reduced almost 40%. 5 Apart from temporary muscular fatigue during the first weeks, no side-effects were seen. 6 Atenolol is an effective blood pressure lowering drug in mild and moderate hypertension, but the drop in blood pressure is associated with marked reduction in heart rate and cardiac output at rest as well as during exercise.", "PMID": 973971}
{"id": "pubmed23n0032_9603", "title": "The effect of pindolol on plasma renin activity and blood pressure in hypertensive patients.", "content": "1 The effect of pindolol administered to twenty-six patients with hypertension of unknown origin was compared with respect to blood pressure and plasma renin activity change after increase of the dose over a period of 6 weeks. 2 There was no clear correlation between the fall of plasma renin activity, which in some patients was very marked, and the fall in blood pressure. Some patients with a fall in plasma renin activity did not drop their pressure. Conversely, some with a fall of pressure did not drop their plasma renin activity. 3 The addition of hydrochlorothiazide to the pindolol finally caused further lowering of the blood pressure in all but one patient and the plasma renin activity rose in all but two patinets. There was no clear correlation between change in plasma renin activity and the effect on blood pressure.", "contents": "The effect of pindolol on plasma renin activity and blood pressure in hypertensive patients. 1 The effect of pindolol administered to twenty-six patients with hypertension of unknown origin was compared with respect to blood pressure and plasma renin activity change after increase of the dose over a period of 6 weeks. 2 There was no clear correlation between the fall of plasma renin activity, which in some patients was very marked, and the fall in blood pressure. Some patients with a fall in plasma renin activity did not drop their pressure. Conversely, some with a fall of pressure did not drop their plasma renin activity. 3 The addition of hydrochlorothiazide to the pindolol finally caused further lowering of the blood pressure in all but one patient and the plasma renin activity rose in all but two patinets. There was no clear correlation between change in plasma renin activity and the effect on blood pressure.", "PMID": 973972}
{"id": "pubmed23n0032_9604", "title": "Ethanol oxidizing enzyme activites in liver disease.", "content": "1 The activites of hepatic alcohol dehydrogenase, catalase and the reduced nicotinamide adenine dinucleotide phosphate (NADPH) dependent ethanol oxidizing system were determined in liver biopsies from nine patients with liver disease and seven control subjects with non evidence of liver disease. 2 Alcohol dehydrogenase and catalase activites were significantly lower in the patients with liver disease. 3 The activity of the NADPH dependent ethanol oxidizing system was significantly greater in the patients with liver disease, when its activity was expressed in terms of mg protein or g wet weight liver. 4 It is suggested that the greater activity of the NADPH dependent system may compensate for the low alcohol dehydrogenase activites found in patients with liver disease and maintain normal rates of ethanol metabolism.", "contents": "Ethanol oxidizing enzyme activites in liver disease. 1 The activites of hepatic alcohol dehydrogenase, catalase and the reduced nicotinamide adenine dinucleotide phosphate (NADPH) dependent ethanol oxidizing system were determined in liver biopsies from nine patients with liver disease and seven control subjects with non evidence of liver disease. 2 Alcohol dehydrogenase and catalase activites were significantly lower in the patients with liver disease. 3 The activity of the NADPH dependent ethanol oxidizing system was significantly greater in the patients with liver disease, when its activity was expressed in terms of mg protein or g wet weight liver. 4 It is suggested that the greater activity of the NADPH dependent system may compensate for the low alcohol dehydrogenase activites found in patients with liver disease and maintain normal rates of ethanol metabolism.", "PMID": 973973}
{"id": "pubmed23n0032_9605", "title": "Agonist and antagonist effects of Sar1-ala8--angiotensin II in salt-loaded and salt-depleted normal man.", "content": "1 Three normal subjects were infused with Sar1-ala8-angiotensin II (Saralasin, P113) whilst on a high sodium (200 mEq + normal diet) and a low sodium (10 mEq diet) intake. 2 On the high sodium intake when angiotensin II and plasma renin activity (PRA) were suppressed, P113 infusion (5-10 mug kg-1 min-1) caused a slight rise in BP and a marked drop in urine flow and sodium excretion, with a fall in glomerular filtration rate, and effective renal plasma flow. 3 On the low sodium intake, when angiotensin II and PRA were increased, P113 infusion (5-10 mugkg-1 min-1) caused no change in blood pressure, urine flow or sodium excretion. However, when P113 was infused at an incremental rate starting at 0.25 mug kg-1 min-1 there was a fall in standing BP, which was maximal at an infusion rate of 1 mug kg-1 min-1, and this fall in standing BP was largely abolished as the rate of infusion was increased to 10 mug kg-1 min -1. 4 These results show firstly that angiotension II is involved in maintaning standing blood pressure during dietary sodium depletion in normal man and secondly that P113 does have agonist as well as antagonist activity in normal man, the effect depending on the level of angiotension II and sodium intake. When looking for angiotensin II mediated hypertension it may ne important to use an incremental rate of infusion of P113 as the agonist activity of larger doses may mask its hypotensive action.", "contents": "Agonist and antagonist effects of Sar1-ala8--angiotensin II in salt-loaded and salt-depleted normal man. 1 Three normal subjects were infused with Sar1-ala8-angiotensin II (Saralasin, P113) whilst on a high sodium (200 mEq + normal diet) and a low sodium (10 mEq diet) intake. 2 On the high sodium intake when angiotensin II and plasma renin activity (PRA) were suppressed, P113 infusion (5-10 mug kg-1 min-1) caused a slight rise in BP and a marked drop in urine flow and sodium excretion, with a fall in glomerular filtration rate, and effective renal plasma flow. 3 On the low sodium intake, when angiotensin II and PRA were increased, P113 infusion (5-10 mugkg-1 min-1) caused no change in blood pressure, urine flow or sodium excretion. However, when P113 was infused at an incremental rate starting at 0.25 mug kg-1 min-1 there was a fall in standing BP, which was maximal at an infusion rate of 1 mug kg-1 min-1, and this fall in standing BP was largely abolished as the rate of infusion was increased to 10 mug kg-1 min -1. 4 These results show firstly that angiotension II is involved in maintaning standing blood pressure during dietary sodium depletion in normal man and secondly that P113 does have agonist as well as antagonist activity in normal man, the effect depending on the level of angiotension II and sodium intake. When looking for angiotensin II mediated hypertension it may ne important to use an incremental rate of infusion of P113 as the agonist activity of larger doses may mask its hypotensive action.", "PMID": 973974}
{"id": "pubmed23n0032_9606", "title": "Studies on the metabolism of meptazinol, a new analgesic drug.", "content": "1 The absorption, metabolism and excretion of the new analgesic meptazinol has been studied in male volunteers following oral and intravenous administration of a mixture of the [1-14C] and [7-3H] labelled compound. 2 After oral dosage, absorption from the gastrointestinal tract was rapid as evidenced by the early attainment of peak plasma radioactivity levels and near complete as shown by only small amounts of radioactivity recovered in the faeces. 3 Although the absorption of the drug was good, the systemic bioavailability was relatively low. Plasma levels of the unchanged drug remained below the limit of detection (20 ng/ml) after an oral dose of 200 mg. However, after intravenous administration of only 20 mg the peak plasma level was approximately 58 ng/ml. Subsequent elimination was rapid and proceeded in an apparently mono exponential manner with a half-life of approximately 2 hours. 4 Excretion of radioactivity was rapid irrespective of the dosage route and took place chiefly via the urine. Over 60% of the administered radioactivity was recovered in the 0-24 h urine collection. Less than 10% of the administered dose was excreted in the faeces. 5 Less than 5% of the drugs was excreted unchanged. The major metabolite appeared to be the glucuronide conjugate of the parent drug. No evidence was found for N-demethylation of the compound. A minor metabolite of the drug which accounted for approximately 7% of the recovered radioactivity has been tentatively identified as 6-ethyl - 6 - (3-hydroxyphenyl) - 1 - methyl-hexahydroazepin - (2H)-2-ONE.", "contents": "Studies on the metabolism of meptazinol, a new analgesic drug. 1 The absorption, metabolism and excretion of the new analgesic meptazinol has been studied in male volunteers following oral and intravenous administration of a mixture of the [1-14C] and [7-3H] labelled compound. 2 After oral dosage, absorption from the gastrointestinal tract was rapid as evidenced by the early attainment of peak plasma radioactivity levels and near complete as shown by only small amounts of radioactivity recovered in the faeces. 3 Although the absorption of the drug was good, the systemic bioavailability was relatively low. Plasma levels of the unchanged drug remained below the limit of detection (20 ng/ml) after an oral dose of 200 mg. However, after intravenous administration of only 20 mg the peak plasma level was approximately 58 ng/ml. Subsequent elimination was rapid and proceeded in an apparently mono exponential manner with a half-life of approximately 2 hours. 4 Excretion of radioactivity was rapid irrespective of the dosage route and took place chiefly via the urine. Over 60% of the administered radioactivity was recovered in the 0-24 h urine collection. Less than 10% of the administered dose was excreted in the faeces. 5 Less than 5% of the drugs was excreted unchanged. The major metabolite appeared to be the glucuronide conjugate of the parent drug. No evidence was found for N-demethylation of the compound. A minor metabolite of the drug which accounted for approximately 7% of the recovered radioactivity has been tentatively identified as 6-ethyl - 6 - (3-hydroxyphenyl) - 1 - methyl-hexahydroazepin - (2H)-2-ONE.", "PMID": 973975}
{"id": "pubmed23n0032_9607", "title": "Modification by diazepam or thioridazine of the psychomotor skills related to driving: a subacute trial in neurotic out-patients.", "content": "Forty-five out-patients with clinically manifested anxiety were tested in order to study the effects of 2 weeks' treatment with placebo, diazepam (5-10 mg three times daily) or thioridazine (25-50 mg three times daily) on their psychomotor skills related to driving. When compared with placebo, diazepam increased the number of mistakes in reaction and co-ordination tests and also decreased ability to discriminate the fusion of flickering light. When compared to other groups, reactive and co-ordinative skills were more impaired in patients treated with thioridazine which also impaired divided attention. Aubjectively thioridazine was not experienced as effective an anxiolytic as diazepam.", "contents": "Modification by diazepam or thioridazine of the psychomotor skills related to driving: a subacute trial in neurotic out-patients. Forty-five out-patients with clinically manifested anxiety were tested in order to study the effects of 2 weeks' treatment with placebo, diazepam (5-10 mg three times daily) or thioridazine (25-50 mg three times daily) on their psychomotor skills related to driving. When compared with placebo, diazepam increased the number of mistakes in reaction and co-ordination tests and also decreased ability to discriminate the fusion of flickering light. When compared to other groups, reactive and co-ordinative skills were more impaired in patients treated with thioridazine which also impaired divided attention. Aubjectively thioridazine was not experienced as effective an anxiolytic as diazepam.", "PMID": 973981}
{"id": "pubmed23n0032_9608", "title": "Distribution and removal of human serum albumin-technetium 99m instilled intranasally.", "content": "The efficacy of antiviral drugs and vaccines administered intranasally may depend upon the technique of application. The distribution and time-course of removal of human serum albumin-technetium 99m (HSA-Tc 99m)-instilled intranasally were studied in eleven healthy volunteers using a gamma camera and an anterior sodium iodide scintillation detector. In 100 randomized studies material was delivered as drops in the supine position or as a spray to seated subjects. A significantly higher proportion of 'good' distributions (62 in 73 tests) was obtained with drops compared with spray (1 in 27). The volume administered was varied between 0.10 ml and 0.75 ml and the concentration of HSA was changed from 3 to 30% with no significant effect upon the distribution of time-course of removal; pertechnetate in isotonic saline was distributed and removed in a manner comparable to HSA-Tc 99m. Activity recorded by the detector showed an initial rapid fall associated with removal of most of the material from the nasal cavity, followed by a slower decline associated with the removal of material mainly from the anterior region of the nose. A multidose study confirmed that frequent administration by drops is required to maintain a high level of activity in the nasal cavity. Using this technique it should be possible to correlate measurements of antiviral efficacy and vaccines take-rates with certain characteristics of intranasal applicators; such studies may lead to the design of better devices.", "contents": "Distribution and removal of human serum albumin-technetium 99m instilled intranasally. The efficacy of antiviral drugs and vaccines administered intranasally may depend upon the technique of application. The distribution and time-course of removal of human serum albumin-technetium 99m (HSA-Tc 99m)-instilled intranasally were studied in eleven healthy volunteers using a gamma camera and an anterior sodium iodide scintillation detector. In 100 randomized studies material was delivered as drops in the supine position or as a spray to seated subjects. A significantly higher proportion of 'good' distributions (62 in 73 tests) was obtained with drops compared with spray (1 in 27). The volume administered was varied between 0.10 ml and 0.75 ml and the concentration of HSA was changed from 3 to 30% with no significant effect upon the distribution of time-course of removal; pertechnetate in isotonic saline was distributed and removed in a manner comparable to HSA-Tc 99m. Activity recorded by the detector showed an initial rapid fall associated with removal of most of the material from the nasal cavity, followed by a slower decline associated with the removal of material mainly from the anterior region of the nose. A multidose study confirmed that frequent administration by drops is required to maintain a high level of activity in the nasal cavity. Using this technique it should be possible to correlate measurements of antiviral efficacy and vaccines take-rates with certain characteristics of intranasal applicators; such studies may lead to the design of better devices.", "PMID": 973982}
{"id": "pubmed23n0032_9609", "title": "Serum and lung tissue levels of cephradine in thoracic surgery.", "content": "Serum and lung tissue levels in fifteen patients who underwent thoracic surgery were determined by the agar-diffusion plate method after i.m. administration of cephradine (500 mg). The mean value of the serum level 30 to 120 min after administration was 6.5 mug/ml, the mean lung tissue level was 2.6 mug/g. The lung tissue levels reached 40% of the simultaneous serum level. Four patients received cephradine for the treatment of post-operative chest infections. This antibiotic has an important therapeutic role in cases of thoracic-surgical infections.", "contents": "Serum and lung tissue levels of cephradine in thoracic surgery. Serum and lung tissue levels in fifteen patients who underwent thoracic surgery were determined by the agar-diffusion plate method after i.m. administration of cephradine (500 mg). The mean value of the serum level 30 to 120 min after administration was 6.5 mug/ml, the mean lung tissue level was 2.6 mug/g. The lung tissue levels reached 40% of the simultaneous serum level. Four patients received cephradine for the treatment of post-operative chest infections. This antibiotic has an important therapeutic role in cases of thoracic-surgical infections.", "PMID": 973983}
{"id": "pubmed23n0032_9610", "title": "Serum concentrations and bioavailability of rifampicin and isoniazid in combination.", "content": "The bioavailability of rifampicin and isoniazid from formulations containing these drugs in combination has been compared to that from formulations containing either drug alone. No formulation-related differences in either rates or extent of bioavailability were found after administration of each formulation. Mean peak serum concentrations of rifampicin (8.2-11.7 mug/ml) occurring 2 to 4 h after doses of 600 mg, and isoniazid (3.6-4.8 mug/ml) occurring 0.5 to 1 h after doses of 300 mg, were similar to those reported in the literature.", "contents": "Serum concentrations and bioavailability of rifampicin and isoniazid in combination. The bioavailability of rifampicin and isoniazid from formulations containing these drugs in combination has been compared to that from formulations containing either drug alone. No formulation-related differences in either rates or extent of bioavailability were found after administration of each formulation. Mean peak serum concentrations of rifampicin (8.2-11.7 mug/ml) occurring 2 to 4 h after doses of 600 mg, and isoniazid (3.6-4.8 mug/ml) occurring 0.5 to 1 h after doses of 300 mg, were similar to those reported in the literature.", "PMID": 973984}
{"id": "pubmed23n0032_9611", "title": "A radioimmunoassay method for prednisolone: comparison with the competitive protein binding method.", "content": "Cross reactivity of prednisolone with an antiserum raised against dexamethasone-21-hemisuccinate conjugated with bovine serum albumin was utilized to develop a radioimmunoassay for prednisolone in unextracted plasma. The results are in good agreement with those obtained with a standard competitive protein binding method employing prior extraction and separation of the synthetic from endogenous steroids.", "contents": "A radioimmunoassay method for prednisolone: comparison with the competitive protein binding method. Cross reactivity of prednisolone with an antiserum raised against dexamethasone-21-hemisuccinate conjugated with bovine serum albumin was utilized to develop a radioimmunoassay for prednisolone in unextracted plasma. The results are in good agreement with those obtained with a standard competitive protein binding method employing prior extraction and separation of the synthetic from endogenous steroids.", "PMID": 973985}
{"id": "pubmed23n0032_9612", "title": "The relationship between liver volume, antipyrine clearance and indocyanine green clearance before and after phenobarbitone administration in man.", "content": "Liver volume and the clearances of antipyrine and indocyanine green have been measured before and after administration of phenobarbitone (180 mg/day) for 3 weeks to ten healthy subjects. The measurement of liver volume by an ultrasound scanning technique yielded reproducible results which were consistent with predictions of liver size by allometric methods. Before phenobarbitone, antipyrine clearance correlated with liver volume, but there was no correlation between indocyanine green clearance and liver volume. Phenobarbitone administration increased the clearance of antipyrine significantly by 90 +/- 14% but there was no significant change in indocyanine green clearance or liver volume. After phenobarbitone the correlation between antipyrine clearance and liver volume persisted. There was no correlation between indocyanine green clearance and liver volume. These results suggest that in non-medicated subjects some of the difference in antipyrine clearance is due to difference in functional hepatic parenchymal mass and that phenobarbitone increases the drug metabolising capacity per unit of hepatic mass but not total liver size.", "contents": "The relationship between liver volume, antipyrine clearance and indocyanine green clearance before and after phenobarbitone administration in man. Liver volume and the clearances of antipyrine and indocyanine green have been measured before and after administration of phenobarbitone (180 mg/day) for 3 weeks to ten healthy subjects. The measurement of liver volume by an ultrasound scanning technique yielded reproducible results which were consistent with predictions of liver size by allometric methods. Before phenobarbitone, antipyrine clearance correlated with liver volume, but there was no correlation between indocyanine green clearance and liver volume. Phenobarbitone administration increased the clearance of antipyrine significantly by 90 +/- 14% but there was no significant change in indocyanine green clearance or liver volume. After phenobarbitone the correlation between antipyrine clearance and liver volume persisted. There was no correlation between indocyanine green clearance and liver volume. These results suggest that in non-medicated subjects some of the difference in antipyrine clearance is due to difference in functional hepatic parenchymal mass and that phenobarbitone increases the drug metabolising capacity per unit of hepatic mass but not total liver size.", "PMID": 973986}
{"id": "pubmed23n0032_9613", "title": "Clinical pharmacokinetic studies of perphenazine.", "content": "A gas-chromatographic method was used for the study in man of the kinetics of perphenazine (PPZ) and its sulphoxide metabolite (PPZ-SO). Various forms of PPZ administration were applied in eighteen schizophrenic patients and four healthy volunteers. Following an i.v. dose of 5 or 6 mg a considerable fluctuation in the plasma concentration was noted before the exponential elimination phase. The average terminal half-life of PPZ was approximately 9.5 hours. PPZ-SO showed up quickly but in low concentrations. After an oral dose of 6 mg no PPZ was detected in plasma and PPZ-SO only as traces. During continuous oral medication, 12 mg three times daily, a low systemic availability and a high PPZ-SO/PPZ ratio was found suggesting a marked first pass effect. PPZ-enanthate given i.m. fortnightly resulted in PPZ-levels comparable to those seen after continuous oral medication, but PPZ-SO concentration were much lower. No accumulation was observed. The systemic clearance rate (average approximately 100 1/h) was the same after PPZ-enanthate i.m. and PPZ i.v., but varied three-fold individually. Side effects were mostly, but not always, registered concomitant with high plasma levels of PPZ.", "contents": "Clinical pharmacokinetic studies of perphenazine. A gas-chromatographic method was used for the study in man of the kinetics of perphenazine (PPZ) and its sulphoxide metabolite (PPZ-SO). Various forms of PPZ administration were applied in eighteen schizophrenic patients and four healthy volunteers. Following an i.v. dose of 5 or 6 mg a considerable fluctuation in the plasma concentration was noted before the exponential elimination phase. The average terminal half-life of PPZ was approximately 9.5 hours. PPZ-SO showed up quickly but in low concentrations. After an oral dose of 6 mg no PPZ was detected in plasma and PPZ-SO only as traces. During continuous oral medication, 12 mg three times daily, a low systemic availability and a high PPZ-SO/PPZ ratio was found suggesting a marked first pass effect. PPZ-enanthate given i.m. fortnightly resulted in PPZ-levels comparable to those seen after continuous oral medication, but PPZ-SO concentration were much lower. No accumulation was observed. The systemic clearance rate (average approximately 100 1/h) was the same after PPZ-enanthate i.m. and PPZ i.v., but varied three-fold individually. Side effects were mostly, but not always, registered concomitant with high plasma levels of PPZ.", "PMID": 973987}
{"id": "pubmed23n0032_9614", "title": "The ralationship between changes in 5-HT induced platelet aggregation and clinical state in patients treated with fluphenazine.", "content": "Platelet aggregation responses to 5-HT and adenosine diphosphate were examined in a population of eighteen patients treated with fluphenazine decanoate for longer than one year. 5-HT induced aggregation was enhanced in ten subjects. This enhancement was similar to that previously described in patients receiving chlorpromazine. Patients who showed enhanced 5-HT induced aggregation showed less rateable psychopathology and less extrapyramidal side-effects than patients who did not show enhancement. These findings suggest that platelet aggregation responses could be used to identify patients who could be safely withdrawn from long-term neuroleptic therapy.", "contents": "The ralationship between changes in 5-HT induced platelet aggregation and clinical state in patients treated with fluphenazine. Platelet aggregation responses to 5-HT and adenosine diphosphate were examined in a population of eighteen patients treated with fluphenazine decanoate for longer than one year. 5-HT induced aggregation was enhanced in ten subjects. This enhancement was similar to that previously described in patients receiving chlorpromazine. Patients who showed enhanced 5-HT induced aggregation showed less rateable psychopathology and less extrapyramidal side-effects than patients who did not show enhancement. These findings suggest that platelet aggregation responses could be used to identify patients who could be safely withdrawn from long-term neuroleptic therapy.", "PMID": 973988}
{"id": "pubmed23n0032_9615", "title": "Effects of aspirin and prostaglandin E2 on secondary phase aggregation responses of schizophrenic patients treated with chlorpromazine.", "content": "We investigated the effects of oral aspirin 900 mg upon the platelet aggregation responses to 5-hydroxytryptamine, dopamine or N-dimethyl dopamine in schizophrenic patients chronically treated with chlorpromazine. Most subjects showed enchanced aggregation responses (that is appearance of secondary phase aggregation) to these agents prior to aspirin administration. When aggregation was re-tested 23.5 h after aspirin the secondary phase of the enhanced aggregation responses was abolished. Addition of 0.14-0.3 mumol/l prostaglandin E2 15 s after inducing aggregation partially restored the secondary phase. The observed secondary phase of aggregation may be due to increased prostaglandin synthesis or release.", "contents": "Effects of aspirin and prostaglandin E2 on secondary phase aggregation responses of schizophrenic patients treated with chlorpromazine. We investigated the effects of oral aspirin 900 mg upon the platelet aggregation responses to 5-hydroxytryptamine, dopamine or N-dimethyl dopamine in schizophrenic patients chronically treated with chlorpromazine. Most subjects showed enchanced aggregation responses (that is appearance of secondary phase aggregation) to these agents prior to aspirin administration. When aggregation was re-tested 23.5 h after aspirin the secondary phase of the enhanced aggregation responses was abolished. Addition of 0.14-0.3 mumol/l prostaglandin E2 15 s after inducing aggregation partially restored the secondary phase. The observed secondary phase of aggregation may be due to increased prostaglandin synthesis or release.", "PMID": 973989}
{"id": "pubmed23n0032_9616", "title": "Macrophages and lymphoid tissues in mice with concomitant tumour immunity.", "content": "The growth in mice of subcutaneous isografts of any of 5 methylcholanthrene-induced fibrosarcomas was associated with macrophage stimulation, reflected in an increased incidence of DNA-synthesizing cells among marcophages in the uninjected peritoneal cavity. This occurred at some stage with 4 tumours that induced concomitant immunity and one that did not. Some degree of splenomegaly also occurred with all 5 tumours. The spleens of all the tumour-bearing mice showed histological evidence of increased haemopoietic activity. Histological changes in the lymphoid elements of the spleen were very different with different tumours, ranging from lymphoid stimulation to lymphoid atrophy. The lymph nodes draining the sites of primary isografts which induced concomitant immunity showed signs of stimulation in the paracortical areas, followed by plasmacytopoiesis in the medullary areas. Stimulation of the paracortical areas was not detected in the nodes draining sites of injection of a tumour not inducing concomitant immunity. Nodes draining the sites of challenge isografts in mice exhibiting concomitant immunity showed plasmacytopiesis.", "contents": "Macrophages and lymphoid tissues in mice with concomitant tumour immunity. The growth in mice of subcutaneous isografts of any of 5 methylcholanthrene-induced fibrosarcomas was associated with macrophage stimulation, reflected in an increased incidence of DNA-synthesizing cells among marcophages in the uninjected peritoneal cavity. This occurred at some stage with 4 tumours that induced concomitant immunity and one that did not. Some degree of splenomegaly also occurred with all 5 tumours. The spleens of all the tumour-bearing mice showed histological evidence of increased haemopoietic activity. Histological changes in the lymphoid elements of the spleen were very different with different tumours, ranging from lymphoid stimulation to lymphoid atrophy. The lymph nodes draining the sites of primary isografts which induced concomitant immunity showed signs of stimulation in the paracortical areas, followed by plasmacytopoiesis in the medullary areas. Stimulation of the paracortical areas was not detected in the nodes draining sites of injection of a tumour not inducing concomitant immunity. Nodes draining the sites of challenge isografts in mice exhibiting concomitant immunity showed plasmacytopiesis.", "PMID": 973997}
{"id": "pubmed23n0032_9617", "title": "Carcinoembryonic antigen and glucose phosphate isomerase in a human colonic cancer model (GW-39).", "content": "Levels of carcinoembryonic antigen (CEA) and glucose phosphate isomerase (GPI) have been compared in the circulating blood of hamsters bearing intra-muscular grafts of GW-39 human colonic tumour. CEA in the sera of GW-39 tumour-bearing hamsters ranged from 2-6 to 8-4 ng/ml (mean = 4-5 +/- 1-7 ng/ml). GPI in the sera of normal hamsters ranged from 332 to 749 iu/1 (mean = 602 +/- 110 iu/1) while those with 14-week-old intra-muscular grafts of a hamster amelanotic melanoma, (A.Mel.3), or GW-39 human colonic carcinoma had a range of 664 to 1267 iu/1 (mean = 1024 +/- 220 iu/1) and 1430 to 4719 iu/1 (mean = 2065 +/- 601 iu/1) respectively. Thus, the ratio of enzyme activity in GW-39, A.Mel.3, and normal hamsters was 3-4:1-7:1, indicating a significant elevation (P less than 0-01) in animals bearing a human colon carcinoma or a hamster melanoma, with particularly high values obtained in hamsters with GW-39. Sequential determinations of CEA and GPI in a group of hamsters transplanted intra-muscularly with GW-39 tumours revealed that both markers increased proportionately with duration of tumour growth, suggesting that both serum CEA and GPI may be used as measures of tumour growth. The concentration of GPI in GW-39 human colonic carcinoma xenografts was also significantly higher than that measured in normal human colon, primary human colonic cancer, or normal hamster tissues. These results support the view that GPI, in addition to CEA, is a quantitatively increased marker in this tumour model, and is liberated into the circulation in proportion to the increase in tumour mass.", "contents": "Carcinoembryonic antigen and glucose phosphate isomerase in a human colonic cancer model (GW-39). Levels of carcinoembryonic antigen (CEA) and glucose phosphate isomerase (GPI) have been compared in the circulating blood of hamsters bearing intra-muscular grafts of GW-39 human colonic tumour. CEA in the sera of GW-39 tumour-bearing hamsters ranged from 2-6 to 8-4 ng/ml (mean = 4-5 +/- 1-7 ng/ml). GPI in the sera of normal hamsters ranged from 332 to 749 iu/1 (mean = 602 +/- 110 iu/1) while those with 14-week-old intra-muscular grafts of a hamster amelanotic melanoma, (A.Mel.3), or GW-39 human colonic carcinoma had a range of 664 to 1267 iu/1 (mean = 1024 +/- 220 iu/1) and 1430 to 4719 iu/1 (mean = 2065 +/- 601 iu/1) respectively. Thus, the ratio of enzyme activity in GW-39, A.Mel.3, and normal hamsters was 3-4:1-7:1, indicating a significant elevation (P less than 0-01) in animals bearing a human colon carcinoma or a hamster melanoma, with particularly high values obtained in hamsters with GW-39. Sequential determinations of CEA and GPI in a group of hamsters transplanted intra-muscularly with GW-39 tumours revealed that both markers increased proportionately with duration of tumour growth, suggesting that both serum CEA and GPI may be used as measures of tumour growth. The concentration of GPI in GW-39 human colonic carcinoma xenografts was also significantly higher than that measured in normal human colon, primary human colonic cancer, or normal hamster tissues. These results support the view that GPI, in addition to CEA, is a quantitatively increased marker in this tumour model, and is liberated into the circulation in proportion to the increase in tumour mass.", "PMID": 973998}
{"id": "pubmed23n0032_9618", "title": "Altered enzyme expression in \"differentiated\" murine neuroblastoma cells.", "content": "Out of 17 enzymes studied, only 9 were detectable by starch gel electrophoresis in mouse neuroblastoma cells in culture. Prostaglandin E1 (PGE1) and 4(-3-butoxy-4-methoxybenzyl)-2-imidazolidinone (R020-1724), a specific inhibitor of cAMP phosphodiesterase, were used to induce \"differentiation\". Lactate and 6-phosphogluconate dehydrogenases and adenylate kinase were expressed as single bands in untreated neuroblastoma and induced \"differentiated\" cells, but the electrophoretic mobility of these enzymes in PGE1-treated cells was slower than that in malignant and R020-1724-treated cells. Three bands of glucose 6-phosphate dehydrogenase were detectable in PGE1-treated cells, whereas the R020-1724-treated cells had two bands and the untreated neuroblastoma cells had only one band. Aldolase was also expressed as a single band; however, the activity of this enzyme was much higher in PGE1-treated cells, whereas the activity was bately detectable for R020-1724-treated and untreated neuroblastoma cells. Some of the enzymes which are present in vivo are absent in vitro. Alkaline phosphatase is present in brain but is absent in neuroblastoma cells in vivo and in vitro. Two bands each of triose phsophate isomerase, fumarase and aldolase are present in brain, but only one band of these enzymes is present in neuroblastoma cells. Although PGE1 and R020-1724 induce many differentiated functions in neuroblastoma cells in a similar manner, PGE1 appears to change characteristically the expression of several enzymes.", "contents": "Altered enzyme expression in \"differentiated\" murine neuroblastoma cells. Out of 17 enzymes studied, only 9 were detectable by starch gel electrophoresis in mouse neuroblastoma cells in culture. Prostaglandin E1 (PGE1) and 4(-3-butoxy-4-methoxybenzyl)-2-imidazolidinone (R020-1724), a specific inhibitor of cAMP phosphodiesterase, were used to induce \"differentiation\". Lactate and 6-phosphogluconate dehydrogenases and adenylate kinase were expressed as single bands in untreated neuroblastoma and induced \"differentiated\" cells, but the electrophoretic mobility of these enzymes in PGE1-treated cells was slower than that in malignant and R020-1724-treated cells. Three bands of glucose 6-phosphate dehydrogenase were detectable in PGE1-treated cells, whereas the R020-1724-treated cells had two bands and the untreated neuroblastoma cells had only one band. Aldolase was also expressed as a single band; however, the activity of this enzyme was much higher in PGE1-treated cells, whereas the activity was bately detectable for R020-1724-treated and untreated neuroblastoma cells. Some of the enzymes which are present in vivo are absent in vitro. Alkaline phosphatase is present in brain but is absent in neuroblastoma cells in vivo and in vitro. Two bands each of triose phsophate isomerase, fumarase and aldolase are present in brain, but only one band of these enzymes is present in neuroblastoma cells. Although PGE1 and R020-1724 induce many differentiated functions in neuroblastoma cells in a similar manner, PGE1 appears to change characteristically the expression of several enzymes.", "PMID": 973999}
{"id": "pubmed23n0032_9619", "title": "Detailed cytogenetic study of a metastatic bronchial carcinoma.", "content": "Karyotyping and marker analysis of G- and C-banded metaphases from a metastatic bronchial carcinoma revealed a dominant stemline with five markers and four sidelines with additional markers. One to three minute bodies were noted in the majority of cells and these were classified as markers. On the basis of this analysis it was possible to postulate an evolutionary pathway within the tumour whereby the stemline was derived from existing sidelines.", "contents": "Detailed cytogenetic study of a metastatic bronchial carcinoma. Karyotyping and marker analysis of G- and C-banded metaphases from a metastatic bronchial carcinoma revealed a dominant stemline with five markers and four sidelines with additional markers. One to three minute bodies were noted in the majority of cells and these were classified as markers. On the basis of this analysis it was possible to postulate an evolutionary pathway within the tumour whereby the stemline was derived from existing sidelines.", "PMID": 974000}
{"id": "pubmed23n0032_9620", "title": "Relevance of hydroxyproline excretion to bone metastasis in breast cancer.", "content": "In 181 consecutive patients with breast cancer, urinary hydroxyproline excretion has been critically evaluated in conjunction with clinical, biochemical, radiological and scintigraphic parameters. The urinary hydroxyproline/creatinine ratio is a sensitive index of the presence of bone metastases. Urinary hydroxyproline excretion is a reliable method of selecting those patients whose elevated serum alkaline phosphatase is secondary to bone disease rather than liver idsease. The estimation of hydroxyproline excretion furthermore gives information on the activity of bone metastasis, and its response to treatment, which cannot be given by radiological or scintigraphic methods. It is doubtful whether urinary hydroxyproline estimation will help to detect bone metastases before they are apparent on scintigrams. When the bone scan is doubtful, as often occurs in older subjects, hydroxyproline excretion has been found to be helpful in classifying the patient. When scintigraphy is not available, an elevation of hydroxyproline excretion, together with an elevation of Ca/cr ratio or alkaline phosphatase activity, may pre-date by several months the radiological demonstration of osseous metastases.", "contents": "Relevance of hydroxyproline excretion to bone metastasis in breast cancer. In 181 consecutive patients with breast cancer, urinary hydroxyproline excretion has been critically evaluated in conjunction with clinical, biochemical, radiological and scintigraphic parameters. The urinary hydroxyproline/creatinine ratio is a sensitive index of the presence of bone metastases. Urinary hydroxyproline excretion is a reliable method of selecting those patients whose elevated serum alkaline phosphatase is secondary to bone disease rather than liver idsease. The estimation of hydroxyproline excretion furthermore gives information on the activity of bone metastasis, and its response to treatment, which cannot be given by radiological or scintigraphic methods. It is doubtful whether urinary hydroxyproline estimation will help to detect bone metastases before they are apparent on scintigrams. When the bone scan is doubtful, as often occurs in older subjects, hydroxyproline excretion has been found to be helpful in classifying the patient. When scintigraphy is not available, an elevation of hydroxyproline excretion, together with an elevation of Ca/cr ratio or alkaline phosphatase activity, may pre-date by several months the radiological demonstration of osseous metastases.", "PMID": 974001}
{"id": "pubmed23n0032_9621", "title": "Serum ferritin concentration in early breast cancer.", "content": "The concentration of circulating ferritin was measured in 250 normal adult women and 229 women presenting with early breast cancer. Ferritin concentrations are higher in cancer patients than in normal women. Patients with an intial circulating ferritin concentration above 200 mug/1 have a higher tumour recurrence rate during the subsequent 4 years.", "contents": "Serum ferritin concentration in early breast cancer. The concentration of circulating ferritin was measured in 250 normal adult women and 229 women presenting with early breast cancer. Ferritin concentrations are higher in cancer patients than in normal women. Patients with an intial circulating ferritin concentration above 200 mug/1 have a higher tumour recurrence rate during the subsequent 4 years.", "PMID": 974002}
{"id": "pubmed23n0032_9622", "title": "Allogeneic grafts of spontaneous canine melanomas and their cell culture strains in neonatal immunosuppressed dogs.", "content": "Canine melanoma has been transplanted to allogeneic neonatal recipients receiving continuous immunosuppression with anti-lymphocyte serum. One spontaneous melanoma was directly transplanted into 8 recipients, 6 of which developed tumours. 5/5 melanoma cell cultures were transplantable, with 19 tumour takes in 31 allogeneic recipients. Serial passage was performed in the case of two melanomas. Tumour development required continuous immunosuppression and the site was dependent upon the route of inoculation and other factors. Transplanted cell cultures were all amelanotic in vitro and in vivo, except in the case of one melanoma which reverted to a melanotic morphology after in vivo growth.", "contents": "Allogeneic grafts of spontaneous canine melanomas and their cell culture strains in neonatal immunosuppressed dogs. Canine melanoma has been transplanted to allogeneic neonatal recipients receiving continuous immunosuppression with anti-lymphocyte serum. One spontaneous melanoma was directly transplanted into 8 recipients, 6 of which developed tumours. 5/5 melanoma cell cultures were transplantable, with 19 tumour takes in 31 allogeneic recipients. Serial passage was performed in the case of two melanomas. Tumour development required continuous immunosuppression and the site was dependent upon the route of inoculation and other factors. Transplanted cell cultures were all amelanotic in vitro and in vivo, except in the case of one melanoma which reverted to a melanotic morphology after in vivo growth.", "PMID": 974003}
{"id": "pubmed23n0032_9623", "title": "Decreased resistance to intravenous tumour-cell challenge during reticuloendothelial depression following surgery.", "content": "The influence of surgical stress on resistance to i.v. challenge with Walker 256 tumour cells was investigated in rats, with respect to the functional state of the reticuloendothelial system (RES). Phagocytic activity of the RES was evaluated by colloid (gelatinized [131I] \"RE test lipid emulsion\") clearance, and opsonin levels were determined by bioassay. Reticuloendothelial clearance capacity was significantly (P less than 0-05) depressed 60 min following surgery (coeliotomy plus jejunal enterotomy) as quantified by both humoral and cellular parameters of RE function. Phagocytic depression was primarily due to impaired hepatic Kupffer cell function and related to a deficiency in the phagocytic supporting capacity of plasma, also referred to as opsonic or recognition factor (RF) capacity. During the postoperative period of RES colloid clearance depression, pulmonary localization of the blood-borne test particulate matter increased. Rats challenged with 51Cr-labelled viable tumour cells at a dose of 1-0 X 106 i.v., either prior to or during the postoperative period of RE depression, manifested a significant (P less than 0-05) increment in pulmonary localization of the viable tumour cells, and a decrease (P less than 0-05) in hepatic clearance. Evaluation of survival patterns demonstrated a significant (P less than 0-01) decrease in host resistance to i.v. tumour cell challenge (2 X 103 cells) during the postoperative period of RE depression and hypo-opsonaemia. Sham-anaesthetized control animals survived 17-9 +/- 0-8 days, while animals challenged during the period of RE depression survived 7-9 +/- 0-4 days. An increased incidence of respiratory distress and nasal discharge was observed in the animals with impaired survival. Thus, surgical manipulation may transiently compromise RES systemic host defence and may be reflected in an increment in the pulmonary localization of blood-borne tumour cells. The relationship of this altered pattern of tumour cell distribution to the impaired survival remains to be determined, and warrants investigations.", "contents": "Decreased resistance to intravenous tumour-cell challenge during reticuloendothelial depression following surgery. The influence of surgical stress on resistance to i.v. challenge with Walker 256 tumour cells was investigated in rats, with respect to the functional state of the reticuloendothelial system (RES). Phagocytic activity of the RES was evaluated by colloid (gelatinized [131I] \"RE test lipid emulsion\") clearance, and opsonin levels were determined by bioassay. Reticuloendothelial clearance capacity was significantly (P less than 0-05) depressed 60 min following surgery (coeliotomy plus jejunal enterotomy) as quantified by both humoral and cellular parameters of RE function. Phagocytic depression was primarily due to impaired hepatic Kupffer cell function and related to a deficiency in the phagocytic supporting capacity of plasma, also referred to as opsonic or recognition factor (RF) capacity. During the postoperative period of RES colloid clearance depression, pulmonary localization of the blood-borne test particulate matter increased. Rats challenged with 51Cr-labelled viable tumour cells at a dose of 1-0 X 106 i.v., either prior to or during the postoperative period of RE depression, manifested a significant (P less than 0-05) increment in pulmonary localization of the viable tumour cells, and a decrease (P less than 0-05) in hepatic clearance. Evaluation of survival patterns demonstrated a significant (P less than 0-01) decrease in host resistance to i.v. tumour cell challenge (2 X 103 cells) during the postoperative period of RE depression and hypo-opsonaemia. Sham-anaesthetized control animals survived 17-9 +/- 0-8 days, while animals challenged during the period of RE depression survived 7-9 +/- 0-4 days. An increased incidence of respiratory distress and nasal discharge was observed in the animals with impaired survival. Thus, surgical manipulation may transiently compromise RES systemic host defence and may be reflected in an increment in the pulmonary localization of blood-borne tumour cells. The relationship of this altered pattern of tumour cell distribution to the impaired survival remains to be determined, and warrants investigations.", "PMID": 974004}
{"id": "pubmed23n0032_9624", "title": "The effect of low-dose pre-operative X-irradiation of implanted mouse mammary carcinomas on local recurrence and metastasis.", "content": "Pre-operative X-irradiation of s.c. implanted first-generation mammary tumours in C3H mice, using either 500 rad or two fractions of 350 rad, produced no improvement in the success of surgery in causing local control or in reduction of distant metastases. The metastasis rate was just significantly higher after the two-fraction treatment of the implanted tumour than after surgical removal alone. The results are in agreement with previously published results on carcinomas and a sarcoma but contrast with those for murine lymphomas.", "contents": "The effect of low-dose pre-operative X-irradiation of implanted mouse mammary carcinomas on local recurrence and metastasis. Pre-operative X-irradiation of s.c. implanted first-generation mammary tumours in C3H mice, using either 500 rad or two fractions of 350 rad, produced no improvement in the success of surgery in causing local control or in reduction of distant metastases. The metastasis rate was just significantly higher after the two-fraction treatment of the implanted tumour than after surgical removal alone. The results are in agreement with previously published results on carcinomas and a sarcoma but contrast with those for murine lymphomas.", "PMID": 974005}
{"id": "pubmed23n0032_9625", "title": "Toxicity, distribution and elimination of the cancerostatic lectins abrin and ricin after parenteral injection into mice.", "content": "The survival time of mice after i.v. injection of the cancerostatic lectins, abrin and ricin was recorded. The LD50 dose was found to be 10-13 ng and 55-65 ng per mouse for abrin and ricin, respectively. Increasing amounts of toxin reduced the survival time, reaching a minimum of about 10 h. Lactose injected with ricin, provided partial protection against ricin, as measured by the survival time. Abrin and ricin labelled with 125I, and shown to retain their full toxic activity, were injected into mice. Most of the radioactivity found in the organs was present in the form of intact toxins, at least up to 5 h after injection. After i.v. injection the highest concentration/g tissue was found in spleen, followed by kidneys, heart, liver and thymus. The relative concentration in liver was considerably higher for ricin than for abrin. Similar results were found after i.p. injection. When lactose was administered together with ricin, almost 80% of the ricin injected was found in the liver after 30 min, compared to 48% without lactose, and the amount in other organs was concurrently reduced. The elimination of total radioactivity was much faster for ricin than abrin. The radioactivity found in the urine was largely present in non-trichloroacetic acid precipitable form, indicating that the toxins were extensively degraded before excretion.", "contents": "Toxicity, distribution and elimination of the cancerostatic lectins abrin and ricin after parenteral injection into mice. The survival time of mice after i.v. injection of the cancerostatic lectins, abrin and ricin was recorded. The LD50 dose was found to be 10-13 ng and 55-65 ng per mouse for abrin and ricin, respectively. Increasing amounts of toxin reduced the survival time, reaching a minimum of about 10 h. Lactose injected with ricin, provided partial protection against ricin, as measured by the survival time. Abrin and ricin labelled with 125I, and shown to retain their full toxic activity, were injected into mice. Most of the radioactivity found in the organs was present in the form of intact toxins, at least up to 5 h after injection. After i.v. injection the highest concentration/g tissue was found in spleen, followed by kidneys, heart, liver and thymus. The relative concentration in liver was considerably higher for ricin than for abrin. Similar results were found after i.p. injection. When lactose was administered together with ricin, almost 80% of the ricin injected was found in the liver after 30 min, compared to 48% without lactose, and the amount in other organs was concurrently reduced. The elimination of total radioactivity was much faster for ricin than abrin. The radioactivity found in the urine was largely present in non-trichloroacetic acid precipitable form, indicating that the toxins were extensively degraded before excretion.", "PMID": 974006}
{"id": "pubmed23n0032_9626", "title": "The effect of isoprenaline on induction of tumours by methyl nitrosourea in the salivary and mammary glands of female wistar rats.", "content": "Pretreatment of rats with isoprenaline sulphate (IPR) stimulated DNA synthesis in both salivary and mammary gland tissues. Salivary gland tumours induced by N-methyl-N-nitrosourea (MNU) were observed for the first time in rats, but occurred only in IPR-pretreated animals given MNU during the period of IPR-stimulated DNA synthesis. The cumulative index of MNU-induced mammary tumours and the number of tumours per tumour-bearing rat were increased by IPR-pretreament only if the animals received MNU during the period of IPR-stimulated DNA synthesis.", "contents": "The effect of isoprenaline on induction of tumours by methyl nitrosourea in the salivary and mammary glands of female wistar rats. Pretreatment of rats with isoprenaline sulphate (IPR) stimulated DNA synthesis in both salivary and mammary gland tissues. Salivary gland tumours induced by N-methyl-N-nitrosourea (MNU) were observed for the first time in rats, but occurred only in IPR-pretreated animals given MNU during the period of IPR-stimulated DNA synthesis. The cumulative index of MNU-induced mammary tumours and the number of tumours per tumour-bearing rat were increased by IPR-pretreament only if the animals received MNU during the period of IPR-stimulated DNA synthesis.", "PMID": 974007}
{"id": "pubmed23n0032_9627", "title": "Epithelioma adenoides cysticum: genetic update.", "content": "Epithelioma adenoides cysticum (EAC) is a well-known genodermatosis which follows an autosomal dominant mode of inheritance. Questions still remain, however, concerning an apparent excess of females with EAC. An analysis of nineteen previously published pedigrees and one newly ascertained pedigree, which in the aggregate included over 175 cases of EAC, induced no excess of affected females, but rather, a marked deficit males. This deficiency was not the consequence of sex linkage or decreased viability, and was most evident in large pedigrees and sibships, sibships not including the probands, and late birth orders. In these situations, the penetrance of EAC in male gene carriers was only 50%, but was close to 100% in female gene carries. Under maximum detection conditions, i.e. small pedigrees, small sibships, sibships containing the proband, and early birth orders, the penetrance in males increased to 85% and was again close to 100% in females. These findings suggested that the deficit of affected males was best explained by a comination of lessened expressivity and penetrance, effects which were magnified under situations of poor detection. A review of familial cases of EAC indicated no consistent associated anomalies such as those that characterize the naevoid basal cell carcinoma syndrome.", "contents": "Epithelioma adenoides cysticum: genetic update. Epithelioma adenoides cysticum (EAC) is a well-known genodermatosis which follows an autosomal dominant mode of inheritance. Questions still remain, however, concerning an apparent excess of females with EAC. An analysis of nineteen previously published pedigrees and one newly ascertained pedigree, which in the aggregate included over 175 cases of EAC, induced no excess of affected females, but rather, a marked deficit males. This deficiency was not the consequence of sex linkage or decreased viability, and was most evident in large pedigrees and sibships, sibships not including the probands, and late birth orders. In these situations, the penetrance of EAC in male gene carriers was only 50%, but was close to 100% in female gene carries. Under maximum detection conditions, i.e. small pedigrees, small sibships, sibships containing the proband, and early birth orders, the penetrance in males increased to 85% and was again close to 100% in females. These findings suggested that the deficit of affected males was best explained by a comination of lessened expressivity and penetrance, effects which were magnified under situations of poor detection. A review of familial cases of EAC indicated no consistent associated anomalies such as those that characterize the naevoid basal cell carcinoma syndrome.", "PMID": 974013}
{"id": "pubmed23n0032_9628", "title": "Histopathological spectrum of erythema multiforme.", "content": "Lesions of erythema multiforeme from seventy-five patients have been studied histologically. In addition to peculiar intercellular epidermal oedema, subepidermal separation and a lymphohistiocytic inflammatory infiltrate in the papillary dermis, epidermal cell necrosis was observed in a variable percentage of the lesions. While dermal disturbance was a predominant finding in the macular lesions, focal or generalized keratinocytic necrosis was seen in the macular, papular, bullous and iris lesions in that order of frequency. Significant numbers of eosinophils were present in the inflammatory infiltrate in 60% of the bullous and 28% of the macular lesions. Our findings suggest that erythema nultiforme represents a tissue reaction with spectral expression, one end presenting as a predominantly dermal disturbance and the other merging into the adult type of toxic epidermal necrolysis. Yet, the histological features remain sufficiently characteristic for differentiation from other erythematous and vesiculobullous eruptions.", "contents": "Histopathological spectrum of erythema multiforme. Lesions of erythema multiforeme from seventy-five patients have been studied histologically. In addition to peculiar intercellular epidermal oedema, subepidermal separation and a lymphohistiocytic inflammatory infiltrate in the papillary dermis, epidermal cell necrosis was observed in a variable percentage of the lesions. While dermal disturbance was a predominant finding in the macular lesions, focal or generalized keratinocytic necrosis was seen in the macular, papular, bullous and iris lesions in that order of frequency. Significant numbers of eosinophils were present in the inflammatory infiltrate in 60% of the bullous and 28% of the macular lesions. Our findings suggest that erythema nultiforme represents a tissue reaction with spectral expression, one end presenting as a predominantly dermal disturbance and the other merging into the adult type of toxic epidermal necrolysis. Yet, the histological features remain sufficiently characteristic for differentiation from other erythematous and vesiculobullous eruptions.", "PMID": 974014}
{"id": "pubmed23n0032_9629", "title": "Changes in transepidermal water loss and the composition of epidermal lecithin after applications of pure fatty acid triglycerides to skin of essential fatty acid-deficient rats.", "content": "The importance of various unsaturated fatty acid triglycerides to the repair of faulty skin barrier function was studied in essential fatty acid-deficient rats. Following cutaneous application of the pure triglycerides for up to 5 days, the hitherto high rate of transepidermal water loss, characteristic of essential fatty acid deficiency in rats, was reduced by the triglycerides of linoleic and gamma-linolenic acids. Incorporation of the applied fatty acids into the lecithin of the epidermis accompanied these changes in water loss, indicating that cutaneously applied triglycerides may be metabolized by the skin and incorporated into complex lipids. Other fatty acid triglycerides, including alpha-linolenic, dihomo-gamma-linolenic, arachidonic and omega-7-heneicosatrienoic acid, did not lower the rate of transepidermal water loss, although all were incorporated into epidermal structural lipids. The non-essential oleic acid also had no effect upon the rate of transepidermal water loss. These data suggest that of the two main essential fatty acids that occur in skin, linoleic acid and arachidonic acid, the former specifically plays an important role in regulating barrier function whereas the later may have a separate function, such as serving as a precursor of prostaglandins.", "contents": "Changes in transepidermal water loss and the composition of epidermal lecithin after applications of pure fatty acid triglycerides to skin of essential fatty acid-deficient rats. The importance of various unsaturated fatty acid triglycerides to the repair of faulty skin barrier function was studied in essential fatty acid-deficient rats. Following cutaneous application of the pure triglycerides for up to 5 days, the hitherto high rate of transepidermal water loss, characteristic of essential fatty acid deficiency in rats, was reduced by the triglycerides of linoleic and gamma-linolenic acids. Incorporation of the applied fatty acids into the lecithin of the epidermis accompanied these changes in water loss, indicating that cutaneously applied triglycerides may be metabolized by the skin and incorporated into complex lipids. Other fatty acid triglycerides, including alpha-linolenic, dihomo-gamma-linolenic, arachidonic and omega-7-heneicosatrienoic acid, did not lower the rate of transepidermal water loss, although all were incorporated into epidermal structural lipids. The non-essential oleic acid also had no effect upon the rate of transepidermal water loss. These data suggest that of the two main essential fatty acids that occur in skin, linoleic acid and arachidonic acid, the former specifically plays an important role in regulating barrier function whereas the later may have a separate function, such as serving as a precursor of prostaglandins.", "PMID": 974016}
{"id": "pubmed23n0032_9630", "title": "The diffusion of nandrolone through hydrated human cadaver skin.", "content": "Effective diffusion constants have been determined for the permeation of nandrolone through whole and stripped human abdominal cadaver skin in vitro. From the values obtained, the diffusion constant for nandrolone in the stratum corneum has been inferred. The experimental approach used yields reproducible results for skin samples taken from a single individual. There is also a surprising degree of uniformity in the values obtained for skin from different individuals.", "contents": "The diffusion of nandrolone through hydrated human cadaver skin. Effective diffusion constants have been determined for the permeation of nandrolone through whole and stripped human abdominal cadaver skin in vitro. From the values obtained, the diffusion constant for nandrolone in the stratum corneum has been inferred. The experimental approach used yields reproducible results for skin samples taken from a single individual. There is also a surprising degree of uniformity in the values obtained for skin from different individuals.", "PMID": 974017}
{"id": "pubmed23n0032_9631", "title": "The fate of Staphylococcal exfoliatin in newborn and adult mice.", "content": "The distribution and excretion of the staphylococcal exfoliatin was investigated following in vivo administration of highly purified 125I-labelled exfoliatin fractions to adult and newborn mice. Adult mice excrete approximately one-third of a test dose by 3 hours as compared to a fifteenth of a test dose excreted by newborn mice. Accordingly, blood tracer radioactivity reaches a relatively higher peak and shows a slower decline in newborns than in adults. The urine of adult mice contains considerable biologically active exfoliating material. Both nephrectomized and carbon tetrachloride-poisoned adult mice injected with exfoliatin develop generalized exfoliation whereas comparable doses in untreated controls have no effect. On the other hand, subtotal hepatectomy, followed by injection of exfoliatin, does not lead to exfoliation. We conclude that renal immaturity is a critical factor responsible for the susceptibility of neonates to generalized staphylococcal scalded skin syndrome.", "contents": "The fate of Staphylococcal exfoliatin in newborn and adult mice. The distribution and excretion of the staphylococcal exfoliatin was investigated following in vivo administration of highly purified 125I-labelled exfoliatin fractions to adult and newborn mice. Adult mice excrete approximately one-third of a test dose by 3 hours as compared to a fifteenth of a test dose excreted by newborn mice. Accordingly, blood tracer radioactivity reaches a relatively higher peak and shows a slower decline in newborns than in adults. The urine of adult mice contains considerable biologically active exfoliating material. Both nephrectomized and carbon tetrachloride-poisoned adult mice injected with exfoliatin develop generalized exfoliation whereas comparable doses in untreated controls have no effect. On the other hand, subtotal hepatectomy, followed by injection of exfoliatin, does not lead to exfoliation. We conclude that renal immaturity is a critical factor responsible for the susceptibility of neonates to generalized staphylococcal scalded skin syndrome.", "PMID": 974018}
{"id": "pubmed23n0032_9632", "title": "Hypersensitivity to bacteria in eczema. II. Titre and immunoglobulin class of antibodies to staphylococci and micrococci.", "content": "The amounts of agglutinating antibody to staphylococcal and micrococcal phenol-extracted (probably teichoic acids) and protein antigens, prepared from Baird Parker types S1, SIV and M1, were no greater in eczematous than in control persons. Most antibody to phenol-extracted to phenol-extracted antigens was IgM which frequently activated complement to lyse red cells which had adsorbed the bacterial antigen. IgG antibody against phenol-extracted staphylococcal antigen (Staph. aureus, Baird Parker Type S1) was shown to be specific antibody combining by the F(ab)2 portion of the molecule. The phenol-extracts did not contain staphylococcal Protein A that binds to the Fc portion of IgG. Furthermore, the presence of Protein A in the protein or total antigen extracts did not appear to modify the results of the tanned cell agglutination test. The occurrence of immediate or of 4 h, Arthus-like skin test responses to staphylococcal or micrococcal antigens was unrelated to the agglutinin or complement-lysis titre of the relevant antibody.", "contents": "Hypersensitivity to bacteria in eczema. II. Titre and immunoglobulin class of antibodies to staphylococci and micrococci. The amounts of agglutinating antibody to staphylococcal and micrococcal phenol-extracted (probably teichoic acids) and protein antigens, prepared from Baird Parker types S1, SIV and M1, were no greater in eczematous than in control persons. Most antibody to phenol-extracted to phenol-extracted antigens was IgM which frequently activated complement to lyse red cells which had adsorbed the bacterial antigen. IgG antibody against phenol-extracted staphylococcal antigen (Staph. aureus, Baird Parker Type S1) was shown to be specific antibody combining by the F(ab)2 portion of the molecule. The phenol-extracts did not contain staphylococcal Protein A that binds to the Fc portion of IgG. Furthermore, the presence of Protein A in the protein or total antigen extracts did not appear to modify the results of the tanned cell agglutination test. The occurrence of immediate or of 4 h, Arthus-like skin test responses to staphylococcal or micrococcal antigens was unrelated to the agglutinin or complement-lysis titre of the relevant antibody.", "PMID": 974019}
{"id": "pubmed23n0032_9633", "title": "Infantile eczema: A long-term follow-up study.", "content": "A 15-17 year follow-up study was conducted on ninety-nine patients who had suffered from infantile eczema. The persistance of the eczema and the occurrence of related conditions were noted. The persistence of eczema was shown to be greater in those patients with a positive family history of eczema and in those who had developed asthma or hay-fever. An attempt was made to see if the persistence of eczema was affected by the position of the child in the family, and some factors provoking relapses were noted. The patients were also questioned with regard to their achievements in academic examinations, and to their social, artistic and sporting activities. The results showed a success rate in examinations not significantly higher than average. It was not possible to show if there is a particular type of atopic personality. There was no constant characteristic in social or artistic patterns. The group as a whole were normal at the milestones of early development, i.e. walking, talking and reading, and also normal with regard to weight and height.", "contents": "Infantile eczema: A long-term follow-up study. A 15-17 year follow-up study was conducted on ninety-nine patients who had suffered from infantile eczema. The persistance of the eczema and the occurrence of related conditions were noted. The persistence of eczema was shown to be greater in those patients with a positive family history of eczema and in those who had developed asthma or hay-fever. An attempt was made to see if the persistence of eczema was affected by the position of the child in the family, and some factors provoking relapses were noted. The patients were also questioned with regard to their achievements in academic examinations, and to their social, artistic and sporting activities. The results showed a success rate in examinations not significantly higher than average. It was not possible to show if there is a particular type of atopic personality. There was no constant characteristic in social or artistic patterns. The group as a whole were normal at the milestones of early development, i.e. walking, talking and reading, and also normal with regard to weight and height.", "PMID": 974022}
{"id": "pubmed23n0032_9634", "title": "Psychological profile of patients with atopic dermatitis.", "content": "Tests designed to measure psychopathological characteristics common to the neurotic population were administered to patients with atopic dermatitis, patients with other dermatological disorders and to a control group of normal individuals. The parameters tested were manifest anxiety, neurosis, extroversion, depression, hypochondriasis and hysteria. The scores were statistically analyzed. The results showed that patients with atopic dermatitis responded significantly differently from patients with other dermatological diseases and from the control group in specific psychometric scales. Moreover, patients with skin conditions other than atopic dermatitis also responded significantly differently from the control group. The study clearly shows that patients with atopic dermatitis have a characteristic psychological profile not shared by the other two groups. The atopic dermatitis patients tend to be in a state of high manifest anxiety, depressed, neurotic and hypochondriac.", "contents": "Psychological profile of patients with atopic dermatitis. Tests designed to measure psychopathological characteristics common to the neurotic population were administered to patients with atopic dermatitis, patients with other dermatological disorders and to a control group of normal individuals. The parameters tested were manifest anxiety, neurosis, extroversion, depression, hypochondriasis and hysteria. The scores were statistically analyzed. The results showed that patients with atopic dermatitis responded significantly differently from patients with other dermatological diseases and from the control group in specific psychometric scales. Moreover, patients with skin conditions other than atopic dermatitis also responded significantly differently from the control group. The study clearly shows that patients with atopic dermatitis have a characteristic psychological profile not shared by the other two groups. The atopic dermatitis patients tend to be in a state of high manifest anxiety, depressed, neurotic and hypochondriac.", "PMID": 974023}
{"id": "pubmed23n0032_9635", "title": "Pigmented lesions in newborn infants.", "content": "1058 newborn infants were examined. Forty-one (3-9%) had clinically discernible pigmented lesions compatible with melanocytic naevi. Biopsy was performed on thirty-four of the forty-one and of these; eleven, representing 1-01% of the infants, proved to be melanocytic naevi. No giant (garment) naevi were seen in this series. Two of the eleven naevi pathologically examined showed histological changes similar to those that have been reported in some giant naevi, but the remaining nine were not only different from criteria usually assigned to giant naevi, but they also differed from the usual adult naevi, in that most were predominantly junctional. None of the melanocytic naevi in this series showed any suggestion of malignant change. In newborn infants it is often impossible clinically to distinguish naevi from other types of pigmented lesions, as only eleven out of the thirty-four pigmented lesions were melanocytic naevi. Seven of the eleven melanocytic naevi were under 1-5 cm in diameter. No pigmented lesions were found on the palms, soles or genitalia.", "contents": "Pigmented lesions in newborn infants. 1058 newborn infants were examined. Forty-one (3-9%) had clinically discernible pigmented lesions compatible with melanocytic naevi. Biopsy was performed on thirty-four of the forty-one and of these; eleven, representing 1-01% of the infants, proved to be melanocytic naevi. No giant (garment) naevi were seen in this series. Two of the eleven naevi pathologically examined showed histological changes similar to those that have been reported in some giant naevi, but the remaining nine were not only different from criteria usually assigned to giant naevi, but they also differed from the usual adult naevi, in that most were predominantly junctional. None of the melanocytic naevi in this series showed any suggestion of malignant change. In newborn infants it is often impossible clinically to distinguish naevi from other types of pigmented lesions, as only eleven out of the thirty-four pigmented lesions were melanocytic naevi. Seven of the eleven melanocytic naevi were under 1-5 cm in diameter. No pigmented lesions were found on the palms, soles or genitalia.", "PMID": 974024}
{"id": "pubmed23n0032_9636", "title": "Subcutaneous fat necrosis of the newborn: Light and electron microscopic studies.", "content": "Specimens from two cases of subcutaneous fat necrosis of the newborn were studied by light and electron microscopy. Crystallization of the fat cell started with clearing of the central fat droplet, followed by the appearance of cylindrical crystals. The fat crystals were phagocytosed by cytoplasmic projections of macrophages as well as by foreign body giant cells. Calcium deposits were demonstrated within the central fat droplet of the degenerated fat cell of Case 2.", "contents": "Subcutaneous fat necrosis of the newborn: Light and electron microscopic studies. Specimens from two cases of subcutaneous fat necrosis of the newborn were studied by light and electron microscopy. Crystallization of the fat cell started with clearing of the central fat droplet, followed by the appearance of cylindrical crystals. The fat crystals were phagocytosed by cytoplasmic projections of macrophages as well as by foreign body giant cells. Calcium deposits were demonstrated within the central fat droplet of the degenerated fat cell of Case 2.", "PMID": 974026}
{"id": "pubmed23n0032_9637", "title": "The effects of photosensitizers and ultraviolet irradiation on the biosynthesis and metabolism of prostaglandins.", "content": "Prostaglandin biosynthesis from arachidonic acid by skin microsomal fraction preparation was enhanced by UV-irradiation at wavelengths of 254 and 360 nm. In the presence of 8-methoxy psoralen (8 MOP) and coal tar, prostaglandin biosynthesis was further enhanced approximately 2-fold by UV-irradiation at 254 nm. Stimulation was less by UV-irradiation at 360 nm. 8-MOP enhanced the conversion of PGE2 into PGF2-9-ketoreductase prepared from skin high speed supernatant fractions. UV-irradiation at 254 nm and 360 nm with or without the photosensitizers had no effect on the activity of the PGE2-9-ketoreductase. These data therefore indicate that the action of UV-irradiation, 8-methoxy psoralen and coal tar on the skin may in part be due to their regulation of the biosynthesis and metabolism of prostaglandins in this tissue.", "contents": "The effects of photosensitizers and ultraviolet irradiation on the biosynthesis and metabolism of prostaglandins. Prostaglandin biosynthesis from arachidonic acid by skin microsomal fraction preparation was enhanced by UV-irradiation at wavelengths of 254 and 360 nm. In the presence of 8-methoxy psoralen (8 MOP) and coal tar, prostaglandin biosynthesis was further enhanced approximately 2-fold by UV-irradiation at 254 nm. Stimulation was less by UV-irradiation at 360 nm. 8-MOP enhanced the conversion of PGE2 into PGF2-9-ketoreductase prepared from skin high speed supernatant fractions. UV-irradiation at 254 nm and 360 nm with or without the photosensitizers had no effect on the activity of the PGE2-9-ketoreductase. These data therefore indicate that the action of UV-irradiation, 8-methoxy psoralen and coal tar on the skin may in part be due to their regulation of the biosynthesis and metabolism of prostaglandins in this tissue.", "PMID": 974025}
{"id": "pubmed23n0032_9638", "title": "Metal corrosion by chloride in sweat. The problem of 'rusters' in industry.", "content": "Workers in the precision engineering industry who readily corrode metal are known as 'rusters'. We have described two patients with this condition, and review current ideas with regard to its cause, detection, prevention and treatment.", "contents": "Metal corrosion by chloride in sweat. The problem of 'rusters' in industry. Workers in the precision engineering industry who readily corrode metal are known as 'rusters'. We have described two patients with this condition, and review current ideas with regard to its cause, detection, prevention and treatment.", "PMID": 974027}
{"id": "pubmed23n0032_9639", "title": "Proprietary hydrocortisone creams. Vasoconstrictor activities and bio-availabilities of six preparations.", "content": "Six proprietary hydrocortisone creams were evaluated for vasoconstrictor activities and bio-availabilities using an occluded blanching test. Statistical analysis showed a significant difference between the formulations. Dioderm, containing 0-1% hydrocortisone, was significantly more active than the 1% hydrocortisone creams--Alphaderm, Calmurid HC, Efcortelan and Vioform-Hydrocortisone. There was no significant difference between Dioderm and Dioderm C. Unlike creams containing more potent corticosteroids the hydrocortisone formulations apparently failed to produce steroid reservoirs in the stratum corneum as assessed by the blanching response.", "contents": "Proprietary hydrocortisone creams. Vasoconstrictor activities and bio-availabilities of six preparations. Six proprietary hydrocortisone creams were evaluated for vasoconstrictor activities and bio-availabilities using an occluded blanching test. Statistical analysis showed a significant difference between the formulations. Dioderm, containing 0-1% hydrocortisone, was significantly more active than the 1% hydrocortisone creams--Alphaderm, Calmurid HC, Efcortelan and Vioform-Hydrocortisone. There was no significant difference between Dioderm and Dioderm C. Unlike creams containing more potent corticosteroids the hydrocortisone formulations apparently failed to produce steroid reservoirs in the stratum corneum as assessed by the blanching response.", "PMID": 974028}
{"id": "pubmed23n0032_9640", "title": "Disseminated superficial actinic porokeratosis. Analysis of an affected family.", "content": "A family is analysed in which disseminated superficial actinic porokeratosis (DSAP) occurred in five members. All three children of one generation are affected. Clinical, histopathological and genetic aspects of DSAP are discussed. Disseminated superficial actinic porokeratosis appears to be a clinical variant of porokeratosis of Mibelli. Since DSAP is a genodermatosis inherited as an autosomal dominant it can occur in any geographical location; however excess sunlight can definitely exacerbate the condition.", "contents": "Disseminated superficial actinic porokeratosis. Analysis of an affected family. A family is analysed in which disseminated superficial actinic porokeratosis (DSAP) occurred in five members. All three children of one generation are affected. Clinical, histopathological and genetic aspects of DSAP are discussed. Disseminated superficial actinic porokeratosis appears to be a clinical variant of porokeratosis of Mibelli. Since DSAP is a genodermatosis inherited as an autosomal dominant it can occur in any geographical location; however excess sunlight can definitely exacerbate the condition.", "PMID": 974029}
{"id": "pubmed23n0032_9641", "title": "Unilateral epidermal naevus resembling acanthosis nigricans.", "content": "A man born with a unilateral epidermal naevus on the right side of the abdomen developed at puberty symmetrical benign acanthosis nigricans in the body folds. The symmetrical eruption later disappeared completely. The unilateral epidermal naevus and the symmetrical eruption showed identical histological features, i.e. those of acanthosis nigricans. At the age of 32 he developed a muco-epidermoid cancer of the left parotid gland. A decision whether the association of unilateral epidermal naevi with malignant tumours occurs with unusual frequency requires (a) a long follow-up of the patient with the unilateral naevus, (b) a differentiation of the unilateral eruption from unilateral malignant acanthosis nigricans and (c) statistical evidence.", "contents": "Unilateral epidermal naevus resembling acanthosis nigricans. A man born with a unilateral epidermal naevus on the right side of the abdomen developed at puberty symmetrical benign acanthosis nigricans in the body folds. The symmetrical eruption later disappeared completely. The unilateral epidermal naevus and the symmetrical eruption showed identical histological features, i.e. those of acanthosis nigricans. At the age of 32 he developed a muco-epidermoid cancer of the left parotid gland. A decision whether the association of unilateral epidermal naevi with malignant tumours occurs with unusual frequency requires (a) a long follow-up of the patient with the unilateral naevus, (b) a differentiation of the unilateral eruption from unilateral malignant acanthosis nigricans and (c) statistical evidence.", "PMID": 974030}
{"id": "pubmed23n0032_9642", "title": "Alpha-thalassaemia trait in various racial groups in the United Kingdom: characterization of a variant of alpha-thalassaemia in Indians.", "content": "Patients whose red-cell indices are suggestive of thalassaemia trait, but who have a normal haemoglobin electrophoretic pattern, may be carriers of alpha-thalassaemia. A diagnosis of alpha-thalassaemia trait was made in 44 such patients, using the incorporation of [3H]leucine by reticulocytes to measure the relative rates of synthesis of the alpha- and beta-chains of adult haemoglobin. Patients with alpha-thalassaemia trait had a reduced rate of synthesis of the alpha-chains, with a mean alpha/beta specific activity ratio of 0.79+/-SD 0.07. The mean alpha/beta specific activity ratio of 20 control subjects was 1.06+/-SD 0.08. The diagnostic value of the haemoglobin H(Hb H) preparation was assessed in proven alpha-thalassaemia heterozygotes of various races. A high proportion of 'false negative' results in Indian and Negro heterozygotes indicated that the Hb H preparations is a highly unreliable screening test for use in a multi-racial population. There was no significant difference in the mean level of Hb A2 in alpha-thalassaemia heterozygotes (2.0+/0SD 0.6) compared with that of the control group (2.1+/0SD0.5). Comparison of data from patients with alpha- and beta-thalassaemia traits showed that alpha-thalssaemia trait is the milder disorder, in terms of its effects of red-cell morphology, red-cell indices and degree of globin chain imbalance. Amongst individual patients with alpha-thalassaemia trait, there was no correlation between the alpha/beta specific activity ratio and the red-blood-cell(RBC) d the red-blood-cell (RBC) count, mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH). This suggests that the alpha/beta ratio cannot be used to distinguish between carriers of a mild gene ('silent carriers') and carriers of a more severe disease. This is the first sutdy to characterize alpha-thalassaemia trait in Indians, in whom it appears to be a common disorder. Haematologically, alpha-thalassaemia trait in Indians is milder than that seen in Chinese and the fact that haemoglobin Bart's hydrops fetalis does not occur in Indians makes it likely that the genetics of Indian alpha-thalassaemia differ from those of the Chinese disease. A possible genetic model for Indian alpha-thalassaemia is discussed and the identification of the homozygote is seen as the first step in the determination of the underlying molecular defect.", "contents": "Alpha-thalassaemia trait in various racial groups in the United Kingdom: characterization of a variant of alpha-thalassaemia in Indians. Patients whose red-cell indices are suggestive of thalassaemia trait, but who have a normal haemoglobin electrophoretic pattern, may be carriers of alpha-thalassaemia. A diagnosis of alpha-thalassaemia trait was made in 44 such patients, using the incorporation of [3H]leucine by reticulocytes to measure the relative rates of synthesis of the alpha- and beta-chains of adult haemoglobin. Patients with alpha-thalassaemia trait had a reduced rate of synthesis of the alpha-chains, with a mean alpha/beta specific activity ratio of 0.79+/-SD 0.07. The mean alpha/beta specific activity ratio of 20 control subjects was 1.06+/-SD 0.08. The diagnostic value of the haemoglobin H(Hb H) preparation was assessed in proven alpha-thalassaemia heterozygotes of various races. A high proportion of 'false negative' results in Indian and Negro heterozygotes indicated that the Hb H preparations is a highly unreliable screening test for use in a multi-racial population. There was no significant difference in the mean level of Hb A2 in alpha-thalassaemia heterozygotes (2.0+/0SD 0.6) compared with that of the control group (2.1+/0SD0.5). Comparison of data from patients with alpha- and beta-thalassaemia traits showed that alpha-thalssaemia trait is the milder disorder, in terms of its effects of red-cell morphology, red-cell indices and degree of globin chain imbalance. Amongst individual patients with alpha-thalassaemia trait, there was no correlation between the alpha/beta specific activity ratio and the red-blood-cell(RBC) d the red-blood-cell (RBC) count, mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH). This suggests that the alpha/beta ratio cannot be used to distinguish between carriers of a mild gene ('silent carriers') and carriers of a more severe disease. This is the first sutdy to characterize alpha-thalassaemia trait in Indians, in whom it appears to be a common disorder. Haematologically, alpha-thalassaemia trait in Indians is milder than that seen in Chinese and the fact that haemoglobin Bart's hydrops fetalis does not occur in Indians makes it likely that the genetics of Indian alpha-thalassaemia differ from those of the Chinese disease. A possible genetic model for Indian alpha-thalassaemia is discussed and the identification of the homozygote is seen as the first step in the determination of the underlying molecular defect.", "PMID": 974033}
{"id": "pubmed23n0032_9643", "title": "Studies of the proporation and synthesis of haemoblogin C Philadelphia in red cells of heterozygotes, a homozygote, and a heterozygote for both haemoglobin G and alpha thalassaemia.", "content": "The proportion of Hb G Philadelphia (alpha68-Asn leads to Lys) in heterozygotes has been found to have a well-defined bimodal distribution around means of 33% and 46% Hb G. microcytosis and hypochromia are consistently associated with the latter group, who also have a decreased ratio of alpha/beta-chain synthesis in the peripheral blood, but these characters are not linked to the Hb-Galpha gene, because a parent with microcytosis and 46% Hb Galpha may have offspring with 33% Hb G without significant microcytosis. In one family a subject with Hb G and Hb G2 but no Hb A or Hb A2 is presumably a homozygote for alphaG. This subject has microcytosis and a decreased ratio of alpha/beta chain synthesis. In another family a subject with Hbs H, G and G2 but without Hbs A or A2 is heterozygous for both Hb G and alpha thalassaemia I. These findings are compatible with the hypothesis that the alphaG mutation occurs on a chromosome with only a single alpha-chain locus and that the expression in heterozygotes as 46% or 33% Hb G is determined by the homologous chromosome in trans having either one or two normal alphaA genes respectively. The significance of this polymorphism for chromosomes carrying alpha-chain genes is discussed.", "contents": "Studies of the proporation and synthesis of haemoblogin C Philadelphia in red cells of heterozygotes, a homozygote, and a heterozygote for both haemoglobin G and alpha thalassaemia. The proportion of Hb G Philadelphia (alpha68-Asn leads to Lys) in heterozygotes has been found to have a well-defined bimodal distribution around means of 33% and 46% Hb G. microcytosis and hypochromia are consistently associated with the latter group, who also have a decreased ratio of alpha/beta-chain synthesis in the peripheral blood, but these characters are not linked to the Hb-Galpha gene, because a parent with microcytosis and 46% Hb Galpha may have offspring with 33% Hb G without significant microcytosis. In one family a subject with Hb G and Hb G2 but no Hb A or Hb A2 is presumably a homozygote for alphaG. This subject has microcytosis and a decreased ratio of alpha/beta chain synthesis. In another family a subject with Hbs H, G and G2 but without Hbs A or A2 is heterozygous for both Hb G and alpha thalassaemia I. These findings are compatible with the hypothesis that the alphaG mutation occurs on a chromosome with only a single alpha-chain locus and that the expression in heterozygotes as 46% or 33% Hb G is determined by the homologous chromosome in trans having either one or two normal alphaA genes respectively. The significance of this polymorphism for chromosomes carrying alpha-chain genes is discussed.", "PMID": 974034}
{"id": "pubmed23n0032_9644", "title": "Iron uptake and ferritin synthesis by peripheral blood leucocytes from normal subjects and patients with iron deficiency and the anaemia of chronic disease.", "content": "The ferritin content of monocytes, lymphocytes and polymorphs is reduced in patients with iron deficiency anaemia. In patients with the anaemia of chronic disease a reduced serum iron concentration is associated with an increase in the ferritin content of all peripheral blood leucocytes. Iron uptake by all cell types is related to transferrin saturation. In iron deficiency anaemia lymphocyte iron uptake is greatly increased, possibly relfecting intracellular iron depletion. In patients with active rheumatoid arthritis and carcinomatosis there is no alteration in leucocyte iron uptake or ferritin synthesis.", "contents": "Iron uptake and ferritin synthesis by peripheral blood leucocytes from normal subjects and patients with iron deficiency and the anaemia of chronic disease. The ferritin content of monocytes, lymphocytes and polymorphs is reduced in patients with iron deficiency anaemia. In patients with the anaemia of chronic disease a reduced serum iron concentration is associated with an increase in the ferritin content of all peripheral blood leucocytes. Iron uptake by all cell types is related to transferrin saturation. In iron deficiency anaemia lymphocyte iron uptake is greatly increased, possibly relfecting intracellular iron depletion. In patients with active rheumatoid arthritis and carcinomatosis there is no alteration in leucocyte iron uptake or ferritin synthesis.", "PMID": 974035}
{"id": "pubmed23n0032_9645", "title": "Chelate mediated transfer of iron from transferrin to desferrioxamine.", "content": "Desferrioxamine, widely used for the treatment of iron overload in Cooley's anaemia, binds iron so tightly that it should quantitatively remove iron from transferrin. Studies conducted in vivo and in vitro, however, have failed to demonstrate significant depletion of transferrin-bound iron by a stoichiometric excess of desferrioxamine. However, low molecular weight chelating agents, capable of forming ternary complexes with transferrin and ferric iron, can promote a rapid transfer of iron from transferrin to desferrioxamine. A possible mechanism for this facilitated exchange is offered.", "contents": "Chelate mediated transfer of iron from transferrin to desferrioxamine. Desferrioxamine, widely used for the treatment of iron overload in Cooley's anaemia, binds iron so tightly that it should quantitatively remove iron from transferrin. Studies conducted in vivo and in vitro, however, have failed to demonstrate significant depletion of transferrin-bound iron by a stoichiometric excess of desferrioxamine. However, low molecular weight chelating agents, capable of forming ternary complexes with transferrin and ferric iron, can promote a rapid transfer of iron from transferrin to desferrioxamine. A possible mechanism for this facilitated exchange is offered.", "PMID": 974036}
{"id": "pubmed23n0032_9646", "title": "Iron absorption in the rat: the search for possible intestinal mucosal carriers.", "content": "The biological relevance of four iron-containing fractions previously detected in rat intestinal mucosal cells has been studied. The distribution of iron in these fractions obtained by chromatography on Sepharose 6B has been examined after in vivo and in vitro incubation of mucosal cells with 59Ce. In addition, the effects of phenobarbitone, cycloheximide, iron-deficiency and iron-loading on the uptake and distrubution of iron within the four mucosal cell fractions was studied. The iron in fraction I was mostly bound to intracellular membrane particles. Fraction II was shown to be ferritin. Fraction III contained some transferrin and also a protein of molecular weight similar to transferrin but which was not precipitable by antitransferrin antiserum. Quantited with the results of 'chaser' experiments suggested that, in addition to ferritin, at least two of the fractions (I and III) were involved in the process of iron absorption by the mucosal cell.", "contents": "Iron absorption in the rat: the search for possible intestinal mucosal carriers. The biological relevance of four iron-containing fractions previously detected in rat intestinal mucosal cells has been studied. The distribution of iron in these fractions obtained by chromatography on Sepharose 6B has been examined after in vivo and in vitro incubation of mucosal cells with 59Ce. In addition, the effects of phenobarbitone, cycloheximide, iron-deficiency and iron-loading on the uptake and distrubution of iron within the four mucosal cell fractions was studied. The iron in fraction I was mostly bound to intracellular membrane particles. Fraction II was shown to be ferritin. Fraction III contained some transferrin and also a protein of molecular weight similar to transferrin but which was not precipitable by antitransferrin antiserum. Quantited with the results of 'chaser' experiments suggested that, in addition to ferritin, at least two of the fractions (I and III) were involved in the process of iron absorption by the mucosal cell.", "PMID": 974037}
{"id": "pubmed23n0032_9647", "title": "Depressed phagocytic function exhibited by polymorphonuclear leucocytes from chronically iron deficient rabbits.", "content": "The production of chronic iron deficiency in rabbits was associated with impaired phagocytosis of opsonized Staphylococcus aureus by their polymorphonuclear leucocytes. These cells had a reduced ability to ingest the bacteria and the rate of ingestion could not be stimulated by autologous serum leucophilic gamma-globulin.", "contents": "Depressed phagocytic function exhibited by polymorphonuclear leucocytes from chronically iron deficient rabbits. The production of chronic iron deficiency in rabbits was associated with impaired phagocytosis of opsonized Staphylococcus aureus by their polymorphonuclear leucocytes. These cells had a reduced ability to ingest the bacteria and the rate of ingestion could not be stimulated by autologous serum leucophilic gamma-globulin.", "PMID": 974038}
{"id": "pubmed23n0032_9648", "title": "A comparison of platelet size, latelet count, and platelet 35S incorporation as assays for thrombopoietin.", "content": "Average platelet size, platelet count, and 35S-incorporation into platelets were compared as methods for the measurement of thrombopoietin-stimulated thrombopoiesis. In mice injected with rabbit anti-mouse platelet serum (RAMPS) average platelet size was shown to be increased as mice were recovering from thrombocytopenia. Also, 35S-measurements on platelets of these mice showed significant increases in cpm/average platelet 2-4 days after RAMPS treatment. Significant increases in 35S-incorporation into the total circulating mass of platelets were found on days 3-4. In normal mice or mice in rebound-thrombocytosis injected with thrombopoietin, platelet size remained unchanged, whereas the platelet cound and 35S-incorporation into platelets were shown to be significantly increased. Moreover, a dose-response experiment in mice pretreated with RAMPS showed a slight increase in platelet count as the dose of TSF was increased, but platelet sizes were unaltered. The % 35S-incorporation into platelets showed a significant linear dose-response, i.e. as the dose of thrombopoietin was increased, as increase in % 35S-incorporation into platelets was observed. These data indicated that of the three indirect measurements of thrombopoietin, the % 35S-incorporation into mouse platelets was the most sensitive, followed by platelet counting; the least sensitive measurement of thrombopoiesis was change in platelet size.", "contents": "A comparison of platelet size, latelet count, and platelet 35S incorporation as assays for thrombopoietin. Average platelet size, platelet count, and 35S-incorporation into platelets were compared as methods for the measurement of thrombopoietin-stimulated thrombopoiesis. In mice injected with rabbit anti-mouse platelet serum (RAMPS) average platelet size was shown to be increased as mice were recovering from thrombocytopenia. Also, 35S-measurements on platelets of these mice showed significant increases in cpm/average platelet 2-4 days after RAMPS treatment. Significant increases in 35S-incorporation into the total circulating mass of platelets were found on days 3-4. In normal mice or mice in rebound-thrombocytosis injected with thrombopoietin, platelet size remained unchanged, whereas the platelet cound and 35S-incorporation into platelets were shown to be significantly increased. Moreover, a dose-response experiment in mice pretreated with RAMPS showed a slight increase in platelet count as the dose of TSF was increased, but platelet sizes were unaltered. The % 35S-incorporation into platelets showed a significant linear dose-response, i.e. as the dose of thrombopoietin was increased, as increase in % 35S-incorporation into platelets was observed. These data indicated that of the three indirect measurements of thrombopoietin, the % 35S-incorporation into mouse platelets was the most sensitive, followed by platelet counting; the least sensitive measurement of thrombopoiesis was change in platelet size.", "PMID": 974039}
{"id": "pubmed23n0032_9649", "title": "Activation of platelets by platelet activating factor (PAF) derived from IgE-sensitized basophils. IV. PAF does not activate platelet factor 3 (PF3).", "content": "Platelet activating factor (PAF) derived from antigen-stimulated, IgE-sensitized rabbit basophils acts on platelets to induce aggregation and secretion of their content of granule-bound vasoactive amines. Despite this, PAF did not activate platelet factor 3. In contrast, collagen induced aggregation, secretion and PF3 activation in the washed platelets. Other stimuli (ADP, C3b, thrombin) also initiated both secretion and PF3 activation. A wide dose range of PAC, including those giving maximal secretion and aggregation, were ineffictive in making PF3 available and the possibility that PAF inhibited PF3, or its generation, was also excluded. It is concluded that PAF is a unique stimulus for platelets and that secretion and aggregation are not necessarily accompanied by PF3 generation.", "contents": "Activation of platelets by platelet activating factor (PAF) derived from IgE-sensitized basophils. IV. PAF does not activate platelet factor 3 (PF3). Platelet activating factor (PAF) derived from antigen-stimulated, IgE-sensitized rabbit basophils acts on platelets to induce aggregation and secretion of their content of granule-bound vasoactive amines. Despite this, PAF did not activate platelet factor 3. In contrast, collagen induced aggregation, secretion and PF3 activation in the washed platelets. Other stimuli (ADP, C3b, thrombin) also initiated both secretion and PF3 activation. A wide dose range of PAC, including those giving maximal secretion and aggregation, were ineffictive in making PF3 available and the possibility that PAF inhibited PF3, or its generation, was also excluded. It is concluded that PAF is a unique stimulus for platelets and that secretion and aggregation are not necessarily accompanied by PF3 generation.", "PMID": 974040}
{"id": "pubmed23n0032_9650", "title": "Fragmentation haemolysis in patients with severe diabetic angiopathy.", "content": "Haemolytic anaemia associated with prominent red cell fragmentation is described in seven patients with long-standing diabetes mellitus. A common freature in the patients was severe microangiopathy as detected by retinal examination and microscopic examination of the kidneys. Renal or pancreatic islet malfunction per se is not involved in the haemolytic syndrome, since red cell abnormalities persisted in one patient for over a year following successful renal and pancreatic transplantation--this, despite the maintenance of normal renal and carbohydrate homeostasis. The kinetics of fragmentation was sutdied by tranfusing snormal type O cells into this type A patient. With reisolation of these cells by the Ashby-technique, rapid and porgressive red cell fragmentation was demonstrated by: (a) membrane lipid loss; (b) osmotic fragility increase; and (c) increase in mean cell haemoblobin concentration. This studies indicate that a red-cell-fragmentation haemolytic anaemia may occur in long-standing diabetes mellitus, related to the angiopathy of this disease and not to insulin deficiency or renal malfunction.", "contents": "Fragmentation haemolysis in patients with severe diabetic angiopathy. Haemolytic anaemia associated with prominent red cell fragmentation is described in seven patients with long-standing diabetes mellitus. A common freature in the patients was severe microangiopathy as detected by retinal examination and microscopic examination of the kidneys. Renal or pancreatic islet malfunction per se is not involved in the haemolytic syndrome, since red cell abnormalities persisted in one patient for over a year following successful renal and pancreatic transplantation--this, despite the maintenance of normal renal and carbohydrate homeostasis. The kinetics of fragmentation was sutdied by tranfusing snormal type O cells into this type A patient. With reisolation of these cells by the Ashby-technique, rapid and porgressive red cell fragmentation was demonstrated by: (a) membrane lipid loss; (b) osmotic fragility increase; and (c) increase in mean cell haemoblobin concentration. This studies indicate that a red-cell-fragmentation haemolytic anaemia may occur in long-standing diabetes mellitus, related to the angiopathy of this disease and not to insulin deficiency or renal malfunction.", "PMID": 974041}
{"id": "pubmed23n0032_9651", "title": "Binding of thrombin to human platelets and its possible significance.", "content": "Thrombin binds tightly to human platelets. The binding reaction is dependent on the thrombin concentration used. At a physiologically significant thrombin concentration, there are about 500 binding sites per platelet with an apparent dissociation constant of 0.02 u/ml. Autoradiography studies of platelets treated with labelled thrombin showed that the thrombin was located on the platelet surface. Separation of the subcellular fractions of platelets treated with labelled thrombin by density gradient centrifugation revealed that the membrane area contained over 80% of the radioactivity initially applied. Furthermore, isolated platelet membranes dind thrombin similar to intact platelets. These data suggest that the receptors for thrombin are located on the platelet membrane. Cytochalasin A, cytochalasin B or prostoglandin E1 did not have any effect on thrombin binding although these agents inhibited platelet aggregation. Thus, binding of thrombin is not sufficient for aggregation of platelets and other steps are involved. These agents do not affect the induction of stimulation but interfere at a later step in the trhombin-platelet interaction. On the other hand, hirudin completely inhibited binding of thrombin to platelets. It appears that the platelet receptor recognizes that part of the thrombin molecule on its surface which hirudin. Binding studies with serotonin loaded platelets showed a close correlation between thrombin binding and the release reaction.", "contents": "Binding of thrombin to human platelets and its possible significance. Thrombin binds tightly to human platelets. The binding reaction is dependent on the thrombin concentration used. At a physiologically significant thrombin concentration, there are about 500 binding sites per platelet with an apparent dissociation constant of 0.02 u/ml. Autoradiography studies of platelets treated with labelled thrombin showed that the thrombin was located on the platelet surface. Separation of the subcellular fractions of platelets treated with labelled thrombin by density gradient centrifugation revealed that the membrane area contained over 80% of the radioactivity initially applied. Furthermore, isolated platelet membranes dind thrombin similar to intact platelets. These data suggest that the receptors for thrombin are located on the platelet membrane. Cytochalasin A, cytochalasin B or prostoglandin E1 did not have any effect on thrombin binding although these agents inhibited platelet aggregation. Thus, binding of thrombin is not sufficient for aggregation of platelets and other steps are involved. These agents do not affect the induction of stimulation but interfere at a later step in the trhombin-platelet interaction. On the other hand, hirudin completely inhibited binding of thrombin to platelets. It appears that the platelet receptor recognizes that part of the thrombin molecule on its surface which hirudin. Binding studies with serotonin loaded platelets showed a close correlation between thrombin binding and the release reaction.", "PMID": 974042}
{"id": "pubmed23n0032_9652", "title": "Factor VIII and human platelet aggregation. III. Further studies on aggregation of humna platelets by neuraminidase-treated human factor VIII.", "content": "When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Upon incubation of human factor VIII with purfied neuraminidase, a very stable platelet aggregating activity develops in the \"Vo-VIII' fraction, not in the \"LMS-FVIII' part. Evidence is provided that the generated aggregating activity is a property of the 'carrier protein' for LMW-F VIII. Desialylated factor VIII retains its antigenic reactivity, its procoagulant or ristocetin cofactor properties and the capacity of its subunits to dissociate and recombine. Neuraminidase-treated human factor VIII, in contrast to intact bovine factor VIII or intact human factor VIII in the presence of restocetin, does not induce aggregation of EDTA-platelet rich plasma, of congenitally afibrinogenaemic platelet rich plasma, nor of washed platelets.", "contents": "Factor VIII and human platelet aggregation. III. Further studies on aggregation of humna platelets by neuraminidase-treated human factor VIII. When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Upon incubation of human factor VIII with purfied neuraminidase, a very stable platelet aggregating activity develops in the \"Vo-VIII' fraction, not in the \"LMS-FVIII' part. Evidence is provided that the generated aggregating activity is a property of the 'carrier protein' for LMW-F VIII. Desialylated factor VIII retains its antigenic reactivity, its procoagulant or ristocetin cofactor properties and the capacity of its subunits to dissociate and recombine. Neuraminidase-treated human factor VIII, in contrast to intact bovine factor VIII or intact human factor VIII in the presence of restocetin, does not induce aggregation of EDTA-platelet rich plasma, of congenitally afibrinogenaemic platelet rich plasma, nor of washed platelets.", "PMID": 974043}
{"id": "pubmed23n0032_9653", "title": "Plasma prolactin levels during pregnancy.", "content": "Plasma prolactin levels in women who were between 8 and 40 weeks pregnant were determined by a homologous double antibody radioimmunoassay method. There were 980 samples from 839 uncomplicated and 213 samples from 116 complicated pregnancies. Prolactin levels in normal pregnancies varied from 6 ng/ml during early pregnancy to 210 ng/ml near term. A few values beyond the normal range were found in various groups of complicated pregnancies. About 14 per cent of samples from patients with threatened abortion and 7 per cent from patients with hypertension were above the upper normal range. The proportion of values below the 10th centile in patients with a low urinary oestrogen excretion was significantly higher than that in the normal population. Plasma prolactin levels did not seem to be a valuable guide to maternal or fetal well-being.", "contents": "Plasma prolactin levels during pregnancy. Plasma prolactin levels in women who were between 8 and 40 weeks pregnant were determined by a homologous double antibody radioimmunoassay method. There were 980 samples from 839 uncomplicated and 213 samples from 116 complicated pregnancies. Prolactin levels in normal pregnancies varied from 6 ng/ml during early pregnancy to 210 ng/ml near term. A few values beyond the normal range were found in various groups of complicated pregnancies. About 14 per cent of samples from patients with threatened abortion and 7 per cent from patients with hypertension were above the upper normal range. The proportion of values below the 10th centile in patients with a low urinary oestrogen excretion was significantly higher than that in the normal population. Plasma prolactin levels did not seem to be a valuable guide to maternal or fetal well-being.", "PMID": 974045}
{"id": "pubmed23n0032_9654", "title": "Six years' experience of the prediction of severity in rhesus haemolytic disease.", "content": "Prediction charts used in the management of patients suffering from rhesus haemolytic disease are presented and the accuracy investigated for two methods of amniotic fluid analysis. In 468 samples from 141 patients there were 7-9 per cent errors of prediction when both methods were used together but in only four cases (2-8 per cent) was the error thought to have possibly contributed to wrong management.", "contents": "Six years' experience of the prediction of severity in rhesus haemolytic disease. Prediction charts used in the management of patients suffering from rhesus haemolytic disease are presented and the accuracy investigated for two methods of amniotic fluid analysis. In 468 samples from 141 patients there were 7-9 per cent errors of prediction when both methods were used together but in only four cases (2-8 per cent) was the error thought to have possibly contributed to wrong management.", "PMID": 974046}
{"id": "pubmed23n0032_9655", "title": "The effect of oral ritodrine on maternal and fetal carbohydrate metabolism.", "content": "The reported growth-promoting effects of the beta-sympathomimetic compound, ritodrine, have been investigated. The carbohydrate tolerance of eight pregnant women was found to be unaffected by treatment with oral ritodrine over a ten-week period. A further observation that the carbohydrate metabolism of the newborn infants of these women was within normal limits tends to discount possibility that any growth-promoting action ritodrine may have on the fetus is mediated through a diabetogenic effect on the mother.", "contents": "The effect of oral ritodrine on maternal and fetal carbohydrate metabolism. The reported growth-promoting effects of the beta-sympathomimetic compound, ritodrine, have been investigated. The carbohydrate tolerance of eight pregnant women was found to be unaffected by treatment with oral ritodrine over a ten-week period. A further observation that the carbohydrate metabolism of the newborn infants of these women was within normal limits tends to discount possibility that any growth-promoting action ritodrine may have on the fetus is mediated through a diabetogenic effect on the mother.", "PMID": 974047}
{"id": "pubmed23n0032_9656", "title": "Further clinical evaluation of the selenomethionine uptake test.", "content": "A review of the selenomethionine uptake ratio as a measure of placental function is presented. Attempts at the early identification of the poorly growing fetus were unsuccessful, and the previously published close correlation of results with clinical findings in later pregnancy has not been confirmed. The theoretical basis of the test has been re-examined. Although modification much reduced the incidence of technically unsatisfactory readings, other difficulties prevented the test from being clinically useful. To some extent the aim of the test has been superseded by concurrent developments and advances in care of the premature baby.", "contents": "Further clinical evaluation of the selenomethionine uptake test. A review of the selenomethionine uptake ratio as a measure of placental function is presented. Attempts at the early identification of the poorly growing fetus were unsuccessful, and the previously published close correlation of results with clinical findings in later pregnancy has not been confirmed. The theoretical basis of the test has been re-examined. Although modification much reduced the incidence of technically unsatisfactory readings, other difficulties prevented the test from being clinically useful. To some extent the aim of the test has been superseded by concurrent developments and advances in care of the premature baby.", "PMID": 974048}
{"id": "pubmed23n0032_9657", "title": "Measurement of intervillous and myometrial blood flow by an intravenous 133Xe method.", "content": "A new 'non-invasive' method for measuring intervillous and myometrial blood flow is presented. After 2 mCi of 133Xe in physiological saline was injected intravenously the patient held her breath for 20 seconds. The tracer entered the placenta as a short bolus and its removal was followed with a scintillation detector. Intervillous and myometrial blood flow per unit volume was calculated from the two-exponential curve. The mean +/-SD intervillous flow in normal pregnancy was 135+/-49 ml/minute/100 ml, the corresponding half time being 0-56+/-0-16 minutes. The myometrial flow was 7-7+/-2-5 ml/minute/100 g. The method gave reproducible results and took only 20 minutes to perform. The dose of radiation to the mother was less than 1 mrad.", "contents": "Measurement of intervillous and myometrial blood flow by an intravenous 133Xe method. A new 'non-invasive' method for measuring intervillous and myometrial blood flow is presented. After 2 mCi of 133Xe in physiological saline was injected intravenously the patient held her breath for 20 seconds. The tracer entered the placenta as a short bolus and its removal was followed with a scintillation detector. Intervillous and myometrial blood flow per unit volume was calculated from the two-exponential curve. The mean +/-SD intervillous flow in normal pregnancy was 135+/-49 ml/minute/100 ml, the corresponding half time being 0-56+/-0-16 minutes. The myometrial flow was 7-7+/-2-5 ml/minute/100 g. The method gave reproducible results and took only 20 minutes to perform. The dose of radiation to the mother was less than 1 mrad.", "PMID": 974049}
{"id": "pubmed23n0032_9658", "title": "Buccal prostaglandin E2 for induction of labour.", "content": "The administration of buccal prostaglandin E2 (PGE2) was evaluated in 30 patients; effective uterine stimulation occurred in 90 per cent of those receiving a dose of 1 mg hourly. Two patients had a prolonged contraction on a single occasion during labour. The unpleasant taste of the tablets and a high incidence of nausea and vomiting indicated that buccal prostaglandins had no advantages over other methods of administering oxytocic agents.", "contents": "Buccal prostaglandin E2 for induction of labour. The administration of buccal prostaglandin E2 (PGE2) was evaluated in 30 patients; effective uterine stimulation occurred in 90 per cent of those receiving a dose of 1 mg hourly. Two patients had a prolonged contraction on a single occasion during labour. The unpleasant taste of the tablets and a high incidence of nausea and vomiting indicated that buccal prostaglandins had no advantages over other methods of administering oxytocic agents.", "PMID": 974050}
{"id": "pubmed23n0032_9659", "title": "Early postpartum tubal ligation under epidural analgesia.", "content": "Tubal ligation under epidural analgesia in the early puerperium (within 10 hours of delivery) was attempted in 51 patients who had an epidural block established during labour. The results are described. No major difficulties were encountered.", "contents": "Early postpartum tubal ligation under epidural analgesia. Tubal ligation under epidural analgesia in the early puerperium (within 10 hours of delivery) was attempted in 51 patients who had an epidural block established during labour. The results are described. No major difficulties were encountered.", "PMID": 974051}
{"id": "pubmed23n0032_9660", "title": "Altered patterns of thyroid hormones in serum and urine in pregnancy and during oral contraceptive therapy.", "content": "Serum total, percentage free fraction and absolute serum free hormone concentration of thyroxine and triiodothyronine were measured in control, pregnant and oral contraceptive users, together with the daily urinary losses of unconjugated thyroid hormones. Increased urinary losses of both hormones, in particular thyroxine, were apparent in pregnancy and these could not be explained in terms either of an increased filtered load of hormone or the presence of proteinuria. The possible existence of filterable small-molecular weight hormone-binding substances in the urine of pregnant patients is discussed. It is concluded that assay of urinary thyroid hormones during pregnancy is of limited diagnostic value because of overlap with thyrotoxic values.", "contents": "Altered patterns of thyroid hormones in serum and urine in pregnancy and during oral contraceptive therapy. Serum total, percentage free fraction and absolute serum free hormone concentration of thyroxine and triiodothyronine were measured in control, pregnant and oral contraceptive users, together with the daily urinary losses of unconjugated thyroid hormones. Increased urinary losses of both hormones, in particular thyroxine, were apparent in pregnancy and these could not be explained in terms either of an increased filtered load of hormone or the presence of proteinuria. The possible existence of filterable small-molecular weight hormone-binding substances in the urine of pregnant patients is discussed. It is concluded that assay of urinary thyroid hormones during pregnancy is of limited diagnostic value because of overlap with thyrotoxic values.", "PMID": 974052}
{"id": "pubmed23n0032_9661", "title": "The scanning electronmicroscopy of human cervical mucus in the non-pregnant and pregnant states.", "content": "The ultrastructure of the high viscosity phase of human cervical mucus was studied by scanning electronmicroscopy. In the normal woman, sequential mucus samples taken throughout the menstrual cycle showed a honeycomb-like structure with interconnecting channels separated by thin membraneous walls. The diameter of the channels in the mucus was 2 to 6 mum in the early follicular phase, 30 to 35 mum in the late follicular phase and 4 to 6 mum in the luteal phase. During the first trimester of pregnancy and also at the beginning and end of the menstrual cycle a compact, dense form of mucus structure was observed. In women using either copper-releasing or pharmacologically inert intrauterine contraceptive devices (IUCDs), the basic structure was similar to that seen in normal women. The channel diameters were normal in women using the inert IUCD but the mucus from women using a copper-releasing IUCD showed a slight decrease in the late follicular phase channel diameter which was dependent on the time the device had been in place. Globular appendages to the membraneous walls of the mucus were observed in the presence of a copper-releasing IUCD.", "contents": "The scanning electronmicroscopy of human cervical mucus in the non-pregnant and pregnant states. The ultrastructure of the high viscosity phase of human cervical mucus was studied by scanning electronmicroscopy. In the normal woman, sequential mucus samples taken throughout the menstrual cycle showed a honeycomb-like structure with interconnecting channels separated by thin membraneous walls. The diameter of the channels in the mucus was 2 to 6 mum in the early follicular phase, 30 to 35 mum in the late follicular phase and 4 to 6 mum in the luteal phase. During the first trimester of pregnancy and also at the beginning and end of the menstrual cycle a compact, dense form of mucus structure was observed. In women using either copper-releasing or pharmacologically inert intrauterine contraceptive devices (IUCDs), the basic structure was similar to that seen in normal women. The channel diameters were normal in women using the inert IUCD but the mucus from women using a copper-releasing IUCD showed a slight decrease in the late follicular phase channel diameter which was dependent on the time the device had been in place. Globular appendages to the membraneous walls of the mucus were observed in the presence of a copper-releasing IUCD.", "PMID": 974053}
{"id": "pubmed23n0032_9662", "title": "Factors influencing absorption of subretinal fluid.", "content": "In 200 cases of retinal detachment successfully treated without drainage of subretinal fluid complete reattachment of the retina was achieved in the first postoperative week in 76 per cent of cases. Delay in subretinal fluid absorption in the remaining 24 per cent of cases was directly related to the duration of the retinal detachment but was not influenced by the patient's age, refractive error, or the characteristics of the detachment.", "contents": "Factors influencing absorption of subretinal fluid. In 200 cases of retinal detachment successfully treated without drainage of subretinal fluid complete reattachment of the retina was achieved in the first postoperative week in 76 per cent of cases. Delay in subretinal fluid absorption in the remaining 24 per cent of cases was directly related to the duration of the retinal detachment but was not influenced by the patient's age, refractive error, or the characteristics of the detachment.", "PMID": 974054}
{"id": "pubmed23n0032_9663", "title": "Trabeculectomy in a Black American glaucoma population.", "content": "Trabeculectomy was performed on 51 Black patients with uncontrolled glaucoma. The operation was successful in 80 per cent of cases followed-up for an average of 18 months. Success seemed to be independent of whether the superficial scleral flap was or was not sutured, although subconjunctival drainage, as indicated by the appearance of a bleb, seemed to be the predominant mechanism whereby the procedure functioned. The success rate in this series is the highest reported for glaucoma filtering procedures in Black glaucoma populations. Trabeculectomy may now be the operation of choice in uncontrolled glaucoma occurring in Black patients.", "contents": "Trabeculectomy in a Black American glaucoma population. Trabeculectomy was performed on 51 Black patients with uncontrolled glaucoma. The operation was successful in 80 per cent of cases followed-up for an average of 18 months. Success seemed to be independent of whether the superficial scleral flap was or was not sutured, although subconjunctival drainage, as indicated by the appearance of a bleb, seemed to be the predominant mechanism whereby the procedure functioned. The success rate in this series is the highest reported for glaucoma filtering procedures in Black glaucoma populations. Trabeculectomy may now be the operation of choice in uncontrolled glaucoma occurring in Black patients.", "PMID": 974055}
{"id": "pubmed23n0032_9664", "title": "Abnormal pupillary responses in myasthenia gravis. A pupillographic study.", "content": "Using an open-loop stimulus (Maxwellian view), the direct pupillary response to light and its derivative curves (velocity and accleration of pupillary reactions) were recorded by infrared video pupillography in seven patients with myasthenia gravis and in three normal subjects. Responses before and after intravenous injection of 5 mg edrophonium hydrochloride were determined. Five of the patients had never taken anticholinesterase drugs except for diagnostic injections of edrophonium while the other two patients had been treated with oral preparations of corticosteroids. Analyses of the pupillary responses showed reduced amplitude, amximal velocity, and maximal acceleration of pupillary constriction in the myasthenic patients. On the other hand, changes in parameters of pupillary dilatation were minimal. Abnormal values returned towards normal within five minutes after edrophonium injection in all patients except in one individual who was being treated with corticosteroids at the time of examination. These results suggest that involvement of the iris sphincter may be common in patients with myasthenia gravis and this can be demonstrated by analysing the pupillary reaction and its derivative curves in response to an open-loop stimulus.", "contents": "Abnormal pupillary responses in myasthenia gravis. A pupillographic study. Using an open-loop stimulus (Maxwellian view), the direct pupillary response to light and its derivative curves (velocity and accleration of pupillary reactions) were recorded by infrared video pupillography in seven patients with myasthenia gravis and in three normal subjects. Responses before and after intravenous injection of 5 mg edrophonium hydrochloride were determined. Five of the patients had never taken anticholinesterase drugs except for diagnostic injections of edrophonium while the other two patients had been treated with oral preparations of corticosteroids. Analyses of the pupillary responses showed reduced amplitude, amximal velocity, and maximal acceleration of pupillary constriction in the myasthenic patients. On the other hand, changes in parameters of pupillary dilatation were minimal. Abnormal values returned towards normal within five minutes after edrophonium injection in all patients except in one individual who was being treated with corticosteroids at the time of examination. These results suggest that involvement of the iris sphincter may be common in patients with myasthenia gravis and this can be demonstrated by analysing the pupillary reaction and its derivative curves in response to an open-loop stimulus.", "PMID": 974056}
{"id": "pubmed23n0032_9665", "title": "Optic nerve metastasis from a mediastinal carcinoid tumour.", "content": "A 25-year-old man with a rapidly progressive metastatic carcinoid tumour arising in the mediastinum developed a metastasis to the optic nerve. This is the first instance in the literature of an ocular metastasis from this variant of carcinoid tumour. The ocular pathology, ultrastructure, and recent information on the derivation of this tumour, believed to originate in the thymus, are presented.", "contents": "Optic nerve metastasis from a mediastinal carcinoid tumour. A 25-year-old man with a rapidly progressive metastatic carcinoid tumour arising in the mediastinum developed a metastasis to the optic nerve. This is the first instance in the literature of an ocular metastasis from this variant of carcinoid tumour. The ocular pathology, ultrastructure, and recent information on the derivation of this tumour, believed to originate in the thymus, are presented.", "PMID": 974058}
{"id": "pubmed23n0032_9666", "title": "Cilia incarnata.", "content": "In connexion with three case reports of cilia incarnata (examples of both cilium incarnatum externum and cilium incarnatum internum), the aetiology and the signs and symptoms of this infrequently recognized anomaly are described.", "contents": "Cilia incarnata. In connexion with three case reports of cilia incarnata (examples of both cilium incarnatum externum and cilium incarnatum internum), the aetiology and the signs and symptoms of this infrequently recognized anomaly are described.", "PMID": 974059}
{"id": "pubmed23n0032_9667", "title": "Recovery of operator DNA binding activity from denatured lactose repressor.", "content": "Lactose repressor monomer subunits, prepared with sodium dodecyl sulfate, have been renatured-reassociated to yield tetrameric repressors active in binding to operator DNA. The majority of moleculesin renatured repressor populations have operator binding properties indistinguishable from the stock, undenatured repressors from which they were prepared. Renaturation of a mixture of monomers from wild-type (Q) and a tight binding repressor yields a population of active repressors containing molecules with operator binding characteristics intermediate to those of the \"parental\" repressor types.", "contents": "Recovery of operator DNA binding activity from denatured lactose repressor. Lactose repressor monomer subunits, prepared with sodium dodecyl sulfate, have been renatured-reassociated to yield tetrameric repressors active in binding to operator DNA. The majority of moleculesin renatured repressor populations have operator binding properties indistinguishable from the stock, undenatured repressors from which they were prepared. Renaturation of a mixture of monomers from wild-type (Q) and a tight binding repressor yields a population of active repressors containing molecules with operator binding characteristics intermediate to those of the \"parental\" repressor types.", "PMID": 974063}
{"id": "pubmed23n0032_9668", "title": "Site of action of ricin on the ribosome.", "content": "The extent of the inhibitory effect of ricin in polyphenylalanine synthesis by eukaryotic ribosomes is strongly dependent upon the concentration of ribosomes and the elongation factors EF 1 and EF2. Maximal inhibition by ricin, in this assay is observed when either ribosomes or the two elongation factors are added under limiting conditions, whereas ricin-treated ribosomes support protein synthesis at saturating concentrations of elongation factors and ribosomes. Similarly, the enzymatic binding of Phe-tRNA to ribosomes is drastically blocked in ricin-treated ribosomes when low EF 1 concentrations are added to the reaction mixture, but there is no inhibition when EF 1 is at saturating concentrations. Furthermore, formation of the complex EF 2-guanosine triphosphate-ribosome, using free ribosomes pretreated with ricin, is strongly inhibited at limiting concentrations of EF2, but is not affected at saturating concentrations of this factor. However, ricin does not inhibit the EF 2-dependent translocation of peptidyl-tRNA by polysomes, although the toxin is very active in preventing amino acid incorporation by polysomes. Our results suggest that the damaging effect of ricin on the ribosome causes a decreased affinity for both elongation factors EF 1 and EF 2. Thus, the toxin inhibits the enzymatic binding of aminoacyl-tRNA to ribosomes. The lack of inhibition of translocation by ricin suggests that the toxin cannot interact with ribosomes with substrate bound to the acceptor site. Essentially similar results are observed with ricin, abrin, ricin A chain, abrin A chain, and ricinus agglutinin A chain. A possible effect of the toxins on initiation and/or termination is further discussed.", "contents": "Site of action of ricin on the ribosome. The extent of the inhibitory effect of ricin in polyphenylalanine synthesis by eukaryotic ribosomes is strongly dependent upon the concentration of ribosomes and the elongation factors EF 1 and EF2. Maximal inhibition by ricin, in this assay is observed when either ribosomes or the two elongation factors are added under limiting conditions, whereas ricin-treated ribosomes support protein synthesis at saturating concentrations of elongation factors and ribosomes. Similarly, the enzymatic binding of Phe-tRNA to ribosomes is drastically blocked in ricin-treated ribosomes when low EF 1 concentrations are added to the reaction mixture, but there is no inhibition when EF 1 is at saturating concentrations. Furthermore, formation of the complex EF 2-guanosine triphosphate-ribosome, using free ribosomes pretreated with ricin, is strongly inhibited at limiting concentrations of EF2, but is not affected at saturating concentrations of this factor. However, ricin does not inhibit the EF 2-dependent translocation of peptidyl-tRNA by polysomes, although the toxin is very active in preventing amino acid incorporation by polysomes. Our results suggest that the damaging effect of ricin on the ribosome causes a decreased affinity for both elongation factors EF 1 and EF 2. Thus, the toxin inhibits the enzymatic binding of aminoacyl-tRNA to ribosomes. The lack of inhibition of translocation by ricin suggests that the toxin cannot interact with ribosomes with substrate bound to the acceptor site. Essentially similar results are observed with ricin, abrin, ricin A chain, abrin A chain, and ricinus agglutinin A chain. A possible effect of the toxins on initiation and/or termination is further discussed.", "PMID": 974064}
{"id": "pubmed23n0032_9669", "title": "Polyribonucleotides containing thiopurines: synthesis and properties of poly (6-thioguanylic acid).", "content": "The synthesis of poly(2-amino-6-chloropurinylic acid) [poly(n2cl6Pu)] by the polynucleotide phosphorylase catalyzed polymerization of 2-amino-6-chloro-9-(beta-D-ribofuranosyl)purine 5'-diphosphate and its chemical conversion to poly(6-thioguanylic acid) [poly(s6G)] is described. Poly(s6G) was found to form a relatively unstable complex with poly(C), the properties of which were incompatible with those previously reported for the same complex prepared by another method [Darlix, J.L., Fromageot, P., and Reich, E. (1973), Biochemistry 12, 914]. It was found that poly(s6G) could be thermally converted to a copolymer of which with poly(C) was strikingly similar to that reported earlier for poly(s6G)-poly(C).", "contents": "Polyribonucleotides containing thiopurines: synthesis and properties of poly (6-thioguanylic acid). The synthesis of poly(2-amino-6-chloropurinylic acid) [poly(n2cl6Pu)] by the polynucleotide phosphorylase catalyzed polymerization of 2-amino-6-chloro-9-(beta-D-ribofuranosyl)purine 5'-diphosphate and its chemical conversion to poly(6-thioguanylic acid) [poly(s6G)] is described. Poly(s6G) was found to form a relatively unstable complex with poly(C), the properties of which were incompatible with those previously reported for the same complex prepared by another method [Darlix, J.L., Fromageot, P., and Reich, E. (1973), Biochemistry 12, 914]. It was found that poly(s6G) could be thermally converted to a copolymer of which with poly(C) was strikingly similar to that reported earlier for poly(s6G)-poly(C).", "PMID": 974065}
{"id": "pubmed23n0032_9670", "title": "Temperature-sensitive DNA polymerase induced by a bacteriophage T5 mutant: relationship between polymerase and exonuclease activities.", "content": "DNA polymerase induced by bacteriophage T5ts53, a mutant with temperature-sensitive polymerase, was purified to about 95% purity as judged by dodecyl sulfate gel electrophoresis. The 3' leads to 5' exonuclease associated with the polymerase had higher activity than that associated with the parent wild-type enzyme. It was more stable to heat than the polymerase, and it degraded primer-template even in the presence of 4 dNTP's at higher temperature. However, the evidence presented shows that the inhibition of DNA synthesis by higher temperature was primarily due to defects in polymerase function rather than to overactive exonuclease. The presence of primer-template DNA stabilized the polymerase to heat. Purified ts53 polymerase was also shown to discriminate against incorportion of BrdUMP, especially at higher temperature. This is an agreement with observations made in vivo with ts53-infected bacteria.", "contents": "Temperature-sensitive DNA polymerase induced by a bacteriophage T5 mutant: relationship between polymerase and exonuclease activities. DNA polymerase induced by bacteriophage T5ts53, a mutant with temperature-sensitive polymerase, was purified to about 95% purity as judged by dodecyl sulfate gel electrophoresis. The 3' leads to 5' exonuclease associated with the polymerase had higher activity than that associated with the parent wild-type enzyme. It was more stable to heat than the polymerase, and it degraded primer-template even in the presence of 4 dNTP's at higher temperature. However, the evidence presented shows that the inhibition of DNA synthesis by higher temperature was primarily due to defects in polymerase function rather than to overactive exonuclease. The presence of primer-template DNA stabilized the polymerase to heat. Purified ts53 polymerase was also shown to discriminate against incorportion of BrdUMP, especially at higher temperature. This is an agreement with observations made in vivo with ts53-infected bacteria.", "PMID": 974066}
{"id": "pubmed23n0032_9671", "title": "The folding and quaternary structure of trimeric 2-keto-3-deoxy-6-phosphogluconic aldolase at 3.5-A resolution.", "content": "An X-ray crystallographic structure determination has been carried out on 2-keto-3-deoxy-6-phosphogluconic (KDPG) aldolase at 3.5-A resolution using the multiple isomorphous replacement method with three heavy atom derivatives along with anomalous dispersion contributions from two of the derivatives. Crystals grown from ammonium sulfate-phosphate buffered (pH 3.5) solutions were: cubic, a= 103.40 (4) A, space group P213. KDPG aldolase consists of trimeric heterologous assemblages utilizing crystallographic threefold symmetry. The overall profile of the oligomeric structure viewed down the threefold axis resembles that of a ship propeller while the subunits are approximate irregular oblate ellipsoids (25 X 45 X 45 A). The folding of most of the polypeptide chain was traced unambiguously. Secondary structural features consist of nine helical regions (75 residues, 35%) and a pair of two parallel chains. The subunit contains a long empty channel which is about 9 X 9 X 30 A with one of the pair of parallel chains forming part of the wall. Three mercury binding sites are located in this channel. These might correspond to the two readily accessible and one of the two buried cysteine residues of each subunit. The channel terminates with another cavity of about 8 X 10 X 25 A near the surface of the oligomeric structure. The regions of the subunits near the threefold axis are characterized by a high degree of secondary structural organization and these make close intersubunit contacts. Quarternary interactions are due mainly to side-chain interactions of helices.", "contents": "The folding and quaternary structure of trimeric 2-keto-3-deoxy-6-phosphogluconic aldolase at 3.5-A resolution. An X-ray crystallographic structure determination has been carried out on 2-keto-3-deoxy-6-phosphogluconic (KDPG) aldolase at 3.5-A resolution using the multiple isomorphous replacement method with three heavy atom derivatives along with anomalous dispersion contributions from two of the derivatives. Crystals grown from ammonium sulfate-phosphate buffered (pH 3.5) solutions were: cubic, a= 103.40 (4) A, space group P213. KDPG aldolase consists of trimeric heterologous assemblages utilizing crystallographic threefold symmetry. The overall profile of the oligomeric structure viewed down the threefold axis resembles that of a ship propeller while the subunits are approximate irregular oblate ellipsoids (25 X 45 X 45 A). The folding of most of the polypeptide chain was traced unambiguously. Secondary structural features consist of nine helical regions (75 residues, 35%) and a pair of two parallel chains. The subunit contains a long empty channel which is about 9 X 9 X 30 A with one of the pair of parallel chains forming part of the wall. Three mercury binding sites are located in this channel. These might correspond to the two readily accessible and one of the two buried cysteine residues of each subunit. The channel terminates with another cavity of about 8 X 10 X 25 A near the surface of the oligomeric structure. The regions of the subunits near the threefold axis are characterized by a high degree of secondary structural organization and these make close intersubunit contacts. Quarternary interactions are due mainly to side-chain interactions of helices.", "PMID": 974067}
{"id": "pubmed23n0032_9672", "title": "Complete amino acid sequence of the myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus.", "content": "The complete amino acid sequence of the major component myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus, was determined by specific cleavage of the protein to obtain large peptides that are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cyanogen bromide at the 2 methionine residues and 4 peptides were obtained by cleaving the methyl acetimidated protein with trypsin at the 3 arginine residues. By subjecting 4 of these peptides and the apomyoglobin to automatic Edman degradation, over 80% of the covalent structure of the protein was obtained. The remainder of the primary structure was determined by further digestion of the central cyanogen bromide peptide with trypsin and staphylococcal protease. This myoglobin differs from that of the sperm whale, Physter catodon, at 15 positions, from that of the California gray whale, Eschrichtius gibbosus, at 14 positions, from that of the common porpoise, Phocoena phocoena, at 6 positions, and from the myoglobin of the Black Sea dolphin, Delphinus delphis and the Amazon River dolphin, Inia goeffrensis, at 5 and 7 positions, respecitvely. All substitutions observed in this sequence fit easily into the tertiary structure of sperm whale myoglobin.", "contents": "Complete amino acid sequence of the myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus. The complete amino acid sequence of the major component myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus, was determined by specific cleavage of the protein to obtain large peptides that are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cyanogen bromide at the 2 methionine residues and 4 peptides were obtained by cleaving the methyl acetimidated protein with trypsin at the 3 arginine residues. By subjecting 4 of these peptides and the apomyoglobin to automatic Edman degradation, over 80% of the covalent structure of the protein was obtained. The remainder of the primary structure was determined by further digestion of the central cyanogen bromide peptide with trypsin and staphylococcal protease. This myoglobin differs from that of the sperm whale, Physter catodon, at 15 positions, from that of the California gray whale, Eschrichtius gibbosus, at 14 positions, from that of the common porpoise, Phocoena phocoena, at 6 positions, and from the myoglobin of the Black Sea dolphin, Delphinus delphis and the Amazon River dolphin, Inia goeffrensis, at 5 and 7 positions, respecitvely. All substitutions observed in this sequence fit easily into the tertiary structure of sperm whale myoglobin.", "PMID": 974068}
{"id": "pubmed23n0032_9673", "title": "Circular dichroism studies on glycogen phosphorylase from rabbit muscle. Interaction with the allosteric activator adenosine 5'-monophosphate.", "content": "Circular dichroism (CD) spectra of glycogen phosphorylase from rabbit muscle have been measured in the presence of various ligands, particularly in the near-ultraviolet wavelength region. Phosphorylases a and b gave similar positive CD spectra as each other, in the 250-310-nm region. The differences in CD between the a and b forms, as well as the CD changes induced by binding of substrate and other ligands except nucleotides to the enzyme, are all relatively small. Binding of AMP and other nucleotides to phosphorylases a and b, and NaBH4-reduced phosphorylase b, however, induces much larger CD spectral changes than the above. The difference CD curve obtained by subtracting the phosphorylase b curve from that of the enzyme- AMP complex is smooth, with a positive maximum at 266 nm and a negative at 289 nm. The results with various other nucleotides show that the induced Cotton effects are dependent on the base chromophore of the nucleotides. The rotational strength of the induced Cotton effect in phosphorylase b by AMP increases under various conditions, under which the affinity of the enzyme for AMP is enhanced, e.g., the addition of glucose 1-phosphate, inorganic phosphate, fluoride ion, divalent metal cations, and spermine, low temperatures, and conversion of the enzyme to the a form. On the contrary, these factors little affect the induced Cotton effects by IMP, GMP, and dAMP. Amylodextrin gave no effect on the extrinsic Cotton effect by binding of AMP plus Mn2+ to phosphorylase b, while it did retard the AMP-induced tetramerization of the enzyme. It is suggested that the interaction of nucleotides with phosphorylase involves stacking between the base ring of the bound nucleotides and an aromatic amino acid residue at the allosteric site of the enzyme, and that, in the high affinity form of the enzyme for AMP, particular bondings are newly formed between the enzyme and the nucleotide allowing the heterotropic cooperativity.", "contents": "Circular dichroism studies on glycogen phosphorylase from rabbit muscle. Interaction with the allosteric activator adenosine 5'-monophosphate. Circular dichroism (CD) spectra of glycogen phosphorylase from rabbit muscle have been measured in the presence of various ligands, particularly in the near-ultraviolet wavelength region. Phosphorylases a and b gave similar positive CD spectra as each other, in the 250-310-nm region. The differences in CD between the a and b forms, as well as the CD changes induced by binding of substrate and other ligands except nucleotides to the enzyme, are all relatively small. Binding of AMP and other nucleotides to phosphorylases a and b, and NaBH4-reduced phosphorylase b, however, induces much larger CD spectral changes than the above. The difference CD curve obtained by subtracting the phosphorylase b curve from that of the enzyme- AMP complex is smooth, with a positive maximum at 266 nm and a negative at 289 nm. The results with various other nucleotides show that the induced Cotton effects are dependent on the base chromophore of the nucleotides. The rotational strength of the induced Cotton effect in phosphorylase b by AMP increases under various conditions, under which the affinity of the enzyme for AMP is enhanced, e.g., the addition of glucose 1-phosphate, inorganic phosphate, fluoride ion, divalent metal cations, and spermine, low temperatures, and conversion of the enzyme to the a form. On the contrary, these factors little affect the induced Cotton effects by IMP, GMP, and dAMP. Amylodextrin gave no effect on the extrinsic Cotton effect by binding of AMP plus Mn2+ to phosphorylase b, while it did retard the AMP-induced tetramerization of the enzyme. It is suggested that the interaction of nucleotides with phosphorylase involves stacking between the base ring of the bound nucleotides and an aromatic amino acid residue at the allosteric site of the enzyme, and that, in the high affinity form of the enzyme for AMP, particular bondings are newly formed between the enzyme and the nucleotide allowing the heterotropic cooperativity.", "PMID": 974069}
{"id": "pubmed23n0032_9674", "title": "Site of action and biphasic effect of neutral salts in the phosphorylase kinase reaction.", "content": "The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase was more sensitive to salt inhibition than the activated form. (2) Ca2+ partially overcame the inhibition of nonactivated kinase. (3) Inhibition by Cl- occurred with either phosphorylase or a tetradecapeptide containing the convertible seryl residue as substrate. (4) Phosphorylation of nonactivated phosphorylase kinase by protein kinase was markedly inhibited by NaNO3, but this salt had little effect on the phosphorylation of histone by protein kinase. The influence of neutral salts on phosphorylase kinase activity was biphasic. Although activity was inhibited at low salt concentrations, it actually was stimulated as the salt concentration was increased. A similar biphasic response to various salt concentrations was observed in the velocities of autophosphorylation of phosphorylase kinase. The lag in the rate of product formation seen during the activity assay was less pronounced at inhibitory salt concentrations and was abolished at stimulatory salt concentrations. How the influence of salts relates to autophosphorylation and the lag is considered.", "contents": "Site of action and biphasic effect of neutral salts in the phosphorylase kinase reaction. The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase was more sensitive to salt inhibition than the activated form. (2) Ca2+ partially overcame the inhibition of nonactivated kinase. (3) Inhibition by Cl- occurred with either phosphorylase or a tetradecapeptide containing the convertible seryl residue as substrate. (4) Phosphorylation of nonactivated phosphorylase kinase by protein kinase was markedly inhibited by NaNO3, but this salt had little effect on the phosphorylation of histone by protein kinase. The influence of neutral salts on phosphorylase kinase activity was biphasic. Although activity was inhibited at low salt concentrations, it actually was stimulated as the salt concentration was increased. A similar biphasic response to various salt concentrations was observed in the velocities of autophosphorylation of phosphorylase kinase. The lag in the rate of product formation seen during the activity assay was less pronounced at inhibitory salt concentrations and was abolished at stimulatory salt concentrations. How the influence of salts relates to autophosphorylation and the lag is considered.", "PMID": 974070}
{"id": "pubmed23n0032_9675", "title": "A detailed structural comparison between the charge relay system in chymotrypsinogen and in alpha-chymotrypsin.", "content": "An improved 2.5-A electron density map of chymotrypsinogen was calculated by incorporating heavy-atom anomalous scattering effects and a new model of the molecule was constructed. Phases from x-ray structure factors (R = 0.43) computed from this model were then used in the calculation of another electron density map against which the model was further refined. The catalytic Ser-195 side chain in the new model is in the \"down\" or \"acyl\" orientation and its Ogamma atom is in position to form a normal hydrogen bond with Nepsilon2 of His-57. In contrast, the corresponding hydrogen bond in alpha-chymotrypsin (Birktoft, J.J., and Blow, D.M. (1972), J.Mol. Biol. 68, 187) is severely distorted, probably as a consequence of a 1.5-A shift in the relative positions of the two cylindrical folding domains composing most of the molecule. We suggest that this activiation induced distortion of the charge-relay, hydrogen-bonding system plays an important role in the genesis of enzymic activity, in accord with an earlier proposal by Wang concerning the role of bent hydrogen bonds in enzyme catalysis (Wang, J.J. (1970), Proc. Natl. Acad. Sci. U.S.A. 66, 874).", "contents": "A detailed structural comparison between the charge relay system in chymotrypsinogen and in alpha-chymotrypsin. An improved 2.5-A electron density map of chymotrypsinogen was calculated by incorporating heavy-atom anomalous scattering effects and a new model of the molecule was constructed. Phases from x-ray structure factors (R = 0.43) computed from this model were then used in the calculation of another electron density map against which the model was further refined. The catalytic Ser-195 side chain in the new model is in the \"down\" or \"acyl\" orientation and its Ogamma atom is in position to form a normal hydrogen bond with Nepsilon2 of His-57. In contrast, the corresponding hydrogen bond in alpha-chymotrypsin (Birktoft, J.J., and Blow, D.M. (1972), J.Mol. Biol. 68, 187) is severely distorted, probably as a consequence of a 1.5-A shift in the relative positions of the two cylindrical folding domains composing most of the molecule. We suggest that this activiation induced distortion of the charge-relay, hydrogen-bonding system plays an important role in the genesis of enzymic activity, in accord with an earlier proposal by Wang concerning the role of bent hydrogen bonds in enzyme catalysis (Wang, J.J. (1970), Proc. Natl. Acad. Sci. U.S.A. 66, 874).", "PMID": 974071}
{"id": "pubmed23n0032_9676", "title": "Microtubule-associated proteins and the stimulation of tubulin assembly in vitro.", "content": "Microtubules, purified by cycles of assembly and disassembly in vitro, are composed of tubulin and several microtubule-associated proteins (MAPs). When the MAPs were separated from the tubulin by phosphocellulose chromatography, the tubulin by phosphocellulose chromatography, the tubulin no longer assembled at 37 degrees C as measured by turbidity. If the MAPs and tubulin were recombined and warmed to 37 degrees C, microtubules assembled. MAPs stimulated tubulin assembly by affecting both the initiation and elongation processes. The effect on initiation was indicated by results showing an increase in initial rate and a decrease in average microtubule length as the MAP:tubulin ratio was increased. The initiation and elongation activities of the MAPs at 4 degrees C during which time the initiating activity decreased while the ability to affect the total amount of assembly remained constant. The decrease in initiating ability was correlated with the loss of the two major components of the MAP fraction, MAPs 1 and 2.", "contents": "Microtubule-associated proteins and the stimulation of tubulin assembly in vitro. Microtubules, purified by cycles of assembly and disassembly in vitro, are composed of tubulin and several microtubule-associated proteins (MAPs). When the MAPs were separated from the tubulin by phosphocellulose chromatography, the tubulin by phosphocellulose chromatography, the tubulin no longer assembled at 37 degrees C as measured by turbidity. If the MAPs and tubulin were recombined and warmed to 37 degrees C, microtubules assembled. MAPs stimulated tubulin assembly by affecting both the initiation and elongation processes. The effect on initiation was indicated by results showing an increase in initial rate and a decrease in average microtubule length as the MAP:tubulin ratio was increased. The initiation and elongation activities of the MAPs at 4 degrees C during which time the initiating activity decreased while the ability to affect the total amount of assembly remained constant. The decrease in initiating ability was correlated with the loss of the two major components of the MAP fraction, MAPs 1 and 2.", "PMID": 974072}
{"id": "pubmed23n0032_9677", "title": "Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes.", "content": "The fluorescence depolarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor the changes in fluidity accompanying the gel-liquid crystalline phase transition in synthetic phosphatidycholine dispersions. The parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles. These parameters are compared with those obtained using other techniques. In addition, the data are interpreted in terms of two limiting molecular models, which in turn offer insight into the structural differences between multilammelar liposomes and small vesicles.", "contents": "Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes. The fluorescence depolarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor the changes in fluidity accompanying the gel-liquid crystalline phase transition in synthetic phosphatidycholine dispersions. The parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles. These parameters are compared with those obtained using other techniques. In addition, the data are interpreted in terms of two limiting molecular models, which in turn offer insight into the structural differences between multilammelar liposomes and small vesicles.", "PMID": 974073}
{"id": "pubmed23n0032_9678", "title": "Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 2 Two-component phosphatidylcholine liposomes.", "content": "The fluorescence deplorarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor changes in fluidity accompanying the gel-liquid crystalline phase transition in phosphatidylcholine dispersions. In this way, the parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles composed of three mixtures of phosphatidycholines: dimyristoyl-dipalmitoyl, dimyristoyl-distearoyl, and dioleyl-dipalmitoyl. Phase diagrams for these mixed-lipid vesicles are constructed by plotting the delimiting temperatures of the phase transition vs. the lipid compostion of the vesicle. The phase diagrams are interpreted to suggest that the miscibilities of the lipids studied are markedly different in small, single-lamellar vesicles and large multilamellar liposomes. These results are discussed in terms of the effects of high curvature on the structure of biological membranes.", "contents": "Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 2 Two-component phosphatidylcholine liposomes. The fluorescence deplorarization associated with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene is used to monitor changes in fluidity accompanying the gel-liquid crystalline phase transition in phosphatidylcholine dispersions. In this way, the parameters of the phase transition are determined for both large, multilamellar liposomes and small, single-lamellar vesicles composed of three mixtures of phosphatidycholines: dimyristoyl-dipalmitoyl, dimyristoyl-distearoyl, and dioleyl-dipalmitoyl. Phase diagrams for these mixed-lipid vesicles are constructed by plotting the delimiting temperatures of the phase transition vs. the lipid compostion of the vesicle. The phase diagrams are interpreted to suggest that the miscibilities of the lipids studied are markedly different in small, single-lamellar vesicles and large multilamellar liposomes. These results are discussed in terms of the effects of high curvature on the structure of biological membranes.", "PMID": 974074}
{"id": "pubmed23n0032_9679", "title": "HeLa cell DNA polymerase gamma: further purification and properties of the enzyme.", "content": "DNA polymerase gamma has been purified over 60 000-fold from HeLa cells which contain no detectable type C viral particles. This purified enzyme shows a specific activity of 25 000 units/mg of protein which is comparable to the known specific activity of homogeneous preparations of human alpha and beta polymerases. The isolated enzyme shows apparent molecular weights ranging from 160 000 to 330 000 according to the method of analysis. The enzyme exhibits optimal activity for copying poly(A) in the presence of 50 mM KPO4 and 130 mM KCl and, under these conditions, copies poly(A) 20 times more rapidly than activated DNA. These assay conditions permit a clear distinction between the gamma-polymerase and DNA polymerase beta which is markedly inhibited by phosphate at this concentration. A comparison of the copying of activated DNA, poly(dA) and poly(A) by DNA polymerases alpha, beta, and gamma under optimal assay conditions for each enzyme is presented. Studies with synthetic and natural nucleic acid templates also show the gamma-polymerase to behave differently that the reverse transcriptases of avian myeloblastosis virus or Rauscher leukemia virus.", "contents": "HeLa cell DNA polymerase gamma: further purification and properties of the enzyme. DNA polymerase gamma has been purified over 60 000-fold from HeLa cells which contain no detectable type C viral particles. This purified enzyme shows a specific activity of 25 000 units/mg of protein which is comparable to the known specific activity of homogeneous preparations of human alpha and beta polymerases. The isolated enzyme shows apparent molecular weights ranging from 160 000 to 330 000 according to the method of analysis. The enzyme exhibits optimal activity for copying poly(A) in the presence of 50 mM KPO4 and 130 mM KCl and, under these conditions, copies poly(A) 20 times more rapidly than activated DNA. These assay conditions permit a clear distinction between the gamma-polymerase and DNA polymerase beta which is markedly inhibited by phosphate at this concentration. A comparison of the copying of activated DNA, poly(dA) and poly(A) by DNA polymerases alpha, beta, and gamma under optimal assay conditions for each enzyme is presented. Studies with synthetic and natural nucleic acid templates also show the gamma-polymerase to behave differently that the reverse transcriptases of avian myeloblastosis virus or Rauscher leukemia virus.", "PMID": 974075}
{"id": "pubmed23n0032_9680", "title": "The distirubtion of active genes (globin) and inactive genes (keratin) in fractionated chicken erythroid chromatin.", "content": "Repeatable fractionation of sheared chromatin from purified populations of chicken erythroid cells has been achieved, based on the Bio-Gel procedure of Janowski et al. ((1972) Karolinska Symp. 5, 112). For reticulocytes, 3-5% of chromatin DNA is excluded from Bio-Gel A-50 m (peak I) and over 90% elutes in the included volume of the column(peak II). Peak I material has a higher protein/DNA ratio than peak II chromatin and the two fractions have melting profiles characteristic of \"active\" and \"inactive\" chromatin, respectively. In cells prelabeled with [3H]uridine or [3H]leucine there was very pronounced preferential association of radioactivity with the \"active\" peak I chromatin. The distribution of \"active\" (globin) and \"inactive\" (keratin) gene sequences in the DNA of fractions from peak I and peak II chromatin was determined with complimentary DNA (cDNA) probes to chicken globin mRNA and chicken feather keratin mRNA. While slight enrichment for globin gene sequences was found in peak I (relative to DNA in these fractions), some 80% of the total globin hybrid formed was found in peak II fractions. Experiments with the keratin cDNA probe showed that these genes were equally distributed in both chromatin fractions rather than being confined to the \"inactive\" peak II material. The hybridization data in particular question the validity of claims for fractionation of chromatin into \"active\" and \"inactive\" material.", "contents": "The distirubtion of active genes (globin) and inactive genes (keratin) in fractionated chicken erythroid chromatin. Repeatable fractionation of sheared chromatin from purified populations of chicken erythroid cells has been achieved, based on the Bio-Gel procedure of Janowski et al. ((1972) Karolinska Symp. 5, 112). For reticulocytes, 3-5% of chromatin DNA is excluded from Bio-Gel A-50 m (peak I) and over 90% elutes in the included volume of the column(peak II). Peak I material has a higher protein/DNA ratio than peak II chromatin and the two fractions have melting profiles characteristic of \"active\" and \"inactive\" chromatin, respectively. In cells prelabeled with [3H]uridine or [3H]leucine there was very pronounced preferential association of radioactivity with the \"active\" peak I chromatin. The distribution of \"active\" (globin) and \"inactive\" (keratin) gene sequences in the DNA of fractions from peak I and peak II chromatin was determined with complimentary DNA (cDNA) probes to chicken globin mRNA and chicken feather keratin mRNA. While slight enrichment for globin gene sequences was found in peak I (relative to DNA in these fractions), some 80% of the total globin hybrid formed was found in peak II fractions. Experiments with the keratin cDNA probe showed that these genes were equally distributed in both chromatin fractions rather than being confined to the \"inactive\" peak II material. The hybridization data in particular question the validity of claims for fractionation of chromatin into \"active\" and \"inactive\" material.", "PMID": 974076}
{"id": "pubmed23n0032_9681", "title": "Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin.", "content": "The hydrated synthetic lecithins, dimyristoyl and dipalmitoyllecithins, undergo two thermal transitions, a broad low enthalpy \"pretransition\" prior to the sharp first-order \"chain-melting\" transition. Both phospholipids exhibit the same temperature-dependent structural changes associated with the thermal pretransition. At low temperatures, below the pretransition, a one-dimensional lamellar lattice is observed. The hydrocarbon chains are fully extended and tilted with respect to the plane of the lipid bilayer. The hydrocarbon chain packing displays a temperature dependence and the angle of tilt of the hydrocarbon chains decreases with increasing temperature, reaching a minimum value of 30 degrees at the pretransition temperature of both lecithins. The pretransition is associated with a structural transformation from a one-dimensional lamellar to a two-dimensional monoclinic lattice consisting of lipid lamellae distorted by a periodic ripple. The hydrocarbon chains remain tilted in the temperature range intermediate between the pretransition and chain-melting transition. The cell parameters of this two-dimensional lattice exhibit a compositional dependence. The a parameter (proportional to the lamellar repeat distance) increases with increasing water content, while the b parameter (a measure of the ripple periodicity) decreases with increasing water content. At the chain-melting transition, the hydrocarbon chains of the phospholipid melt and assume a liquid-like conformation and the lattice reverts to one-dimensional lamellar. These structural changes observed for dimyristoyl- and dipalmitoyllecithins may be a common feature of all synthetic lecithins exhibiting a thermal pretransition. The appearance of the pretransition and accompanying two-dimensional may arise from specific interactions between the choline moiety of the polar head group and the structured water matrix surrounding it.", "contents": "Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. The hydrated synthetic lecithins, dimyristoyl and dipalmitoyllecithins, undergo two thermal transitions, a broad low enthalpy \"pretransition\" prior to the sharp first-order \"chain-melting\" transition. Both phospholipids exhibit the same temperature-dependent structural changes associated with the thermal pretransition. At low temperatures, below the pretransition, a one-dimensional lamellar lattice is observed. The hydrocarbon chains are fully extended and tilted with respect to the plane of the lipid bilayer. The hydrocarbon chain packing displays a temperature dependence and the angle of tilt of the hydrocarbon chains decreases with increasing temperature, reaching a minimum value of 30 degrees at the pretransition temperature of both lecithins. The pretransition is associated with a structural transformation from a one-dimensional lamellar to a two-dimensional monoclinic lattice consisting of lipid lamellae distorted by a periodic ripple. The hydrocarbon chains remain tilted in the temperature range intermediate between the pretransition and chain-melting transition. The cell parameters of this two-dimensional lattice exhibit a compositional dependence. The a parameter (proportional to the lamellar repeat distance) increases with increasing water content, while the b parameter (a measure of the ripple periodicity) decreases with increasing water content. At the chain-melting transition, the hydrocarbon chains of the phospholipid melt and assume a liquid-like conformation and the lattice reverts to one-dimensional lamellar. These structural changes observed for dimyristoyl- and dipalmitoyllecithins may be a common feature of all synthetic lecithins exhibiting a thermal pretransition. The appearance of the pretransition and accompanying two-dimensional may arise from specific interactions between the choline moiety of the polar head group and the structured water matrix surrounding it.", "PMID": 974077}
{"id": "pubmed23n0032_9682", "title": "Elucidation of lectin receptors by quantitative inhibition of lectin binding to human erythrocytes and lymphocytes.", "content": "The binding to normal and sialidase-treated human erythrocytes and lymphocytes of four 125I-labeled lectins [Maackia amurensis hemagglutinins (MAM and MAH), Ricinus communis hemagglutinin (RCH), and Bauhinia purpurea hemagglutinin (BPH)] was studied in detail. The quantitative inhibition assays against the lectin binding to the cells were also performed with various glyco-proteins and glycopeptides as inhibitors. The comparison of the inhibition constants of the inhibitors thus obtained with the association constants of the lectins to the cells permitted estimation of the relative receptor activities of cell surface glyco-proteins toward the lectins.", "contents": "Elucidation of lectin receptors by quantitative inhibition of lectin binding to human erythrocytes and lymphocytes. The binding to normal and sialidase-treated human erythrocytes and lymphocytes of four 125I-labeled lectins [Maackia amurensis hemagglutinins (MAM and MAH), Ricinus communis hemagglutinin (RCH), and Bauhinia purpurea hemagglutinin (BPH)] was studied in detail. The quantitative inhibition assays against the lectin binding to the cells were also performed with various glyco-proteins and glycopeptides as inhibitors. The comparison of the inhibition constants of the inhibitors thus obtained with the association constants of the lectins to the cells permitted estimation of the relative receptor activities of cell surface glyco-proteins toward the lectins.", "PMID": 974078}
{"id": "pubmed23n0032_9683", "title": "Visual-pigment spectra: implications of the protonation of the retinal Schiff base.", "content": "Various models of visual-pigment spectra are critically discussed in terms of the spectral properties of protonated Schiff bases and the common structural features of most proteins. The opsin apoprotein is capable of regulating visual pigment wavelengths in ways that are difficult or impossible to reproduce in model systems. Theories based on solvent effects of the spectra of protonated Schiff bases may be misleading. Careful parameterization using known polyene spectra allows accurate calculation of the spectral properties of protonated Schiff bases. It is shown that an isolated protonated Schiff base of retinal should absorb near 600 nm and that blue-shifted spectra seen in solution arise from associated counterions or solvent molecules. We conclude that the most plausible specific model of chromophore-protein interactions is one in which the protonated Schiff base is closely associated with its counterion and where additional negatively charged or polar groups are positioned by the protein in the vicinity of the ring half of the chromophore. Pigment absorption maxima, bandwidths, and the A2-A1 pigment absorption differences arise naturally from these simple models of pigment spectra.", "contents": "Visual-pigment spectra: implications of the protonation of the retinal Schiff base. Various models of visual-pigment spectra are critically discussed in terms of the spectral properties of protonated Schiff bases and the common structural features of most proteins. The opsin apoprotein is capable of regulating visual pigment wavelengths in ways that are difficult or impossible to reproduce in model systems. Theories based on solvent effects of the spectra of protonated Schiff bases may be misleading. Careful parameterization using known polyene spectra allows accurate calculation of the spectral properties of protonated Schiff bases. It is shown that an isolated protonated Schiff base of retinal should absorb near 600 nm and that blue-shifted spectra seen in solution arise from associated counterions or solvent molecules. We conclude that the most plausible specific model of chromophore-protein interactions is one in which the protonated Schiff base is closely associated with its counterion and where additional negatively charged or polar groups are positioned by the protein in the vicinity of the ring half of the chromophore. Pigment absorption maxima, bandwidths, and the A2-A1 pigment absorption differences arise naturally from these simple models of pigment spectra.", "PMID": 974079}
{"id": "pubmed23n0032_9684", "title": "Hummel-Dreyer gel chromatographic procedure as applied to ligand-mediated association.", "content": "A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand binding in such systems. Guidelines are drawn for unambiguous interpretation of the nonclasical elution profiles in terms of the number of binding sites on the macromonomer and their intrinsic binding constant. Most of these guidelines also apply to static equilibrium methods such as equilibrium dialysis.", "contents": "Hummel-Dreyer gel chromatographic procedure as applied to ligand-mediated association. A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand binding in such systems. Guidelines are drawn for unambiguous interpretation of the nonclasical elution profiles in terms of the number of binding sites on the macromonomer and their intrinsic binding constant. Most of these guidelines also apply to static equilibrium methods such as equilibrium dialysis.", "PMID": 974080}
{"id": "pubmed23n0032_9685", "title": "Poly(uridylic acid) sequences in messenger ribonucleic acid of HeLa cells.", "content": "The poly(uridylic acid [poly(U)] sequences of 30 to 40 nucleotides found in the heterogeneous nuclear ribonucleic acid (RNA) Of HeLa cells are also present in the poly(adenylic acid) [poly(A)] containing messenger RNA (mRNA) of these cells. Twenty-five percent of the total poly(U) sequences in the cell are found in the cytoplasm after HeLa cells have been labeled for 2.5 h with 32P. This value is close to 40% if only poly(A) containing RNA molecules of the cell are considered. The distribution of poly(U) sequences parallels that of poly(A) sequences in different size classes of cytoplasmic messenger RNA. From the concentrations and lengths of the two sequences it can be estimated that about 20% of the poly(A) containing mRNA molecules could contain one poly(U) sequence. Several lines of evidence have been developed to rule out poly(U) containing heterogeneous nuclear RNA (HnRNA) as the source of the poly(U) sequences in cytoplasm. Poly(U) sequences are also found in RNA molecules of the nucleus and cytoplasm which do not contain poly(A). Although poly(U) sequences are most abundant in nuclear RNA species lacking poly(A), they are well represented in a class of RNA molecules in cytoplasm which lack poly(A), but which otherwise resemble the polysomal mRNA of HeLa cells.", "contents": "Poly(uridylic acid) sequences in messenger ribonucleic acid of HeLa cells. The poly(uridylic acid [poly(U)] sequences of 30 to 40 nucleotides found in the heterogeneous nuclear ribonucleic acid (RNA) Of HeLa cells are also present in the poly(adenylic acid) [poly(A)] containing messenger RNA (mRNA) of these cells. Twenty-five percent of the total poly(U) sequences in the cell are found in the cytoplasm after HeLa cells have been labeled for 2.5 h with 32P. This value is close to 40% if only poly(A) containing RNA molecules of the cell are considered. The distribution of poly(U) sequences parallels that of poly(A) sequences in different size classes of cytoplasmic messenger RNA. From the concentrations and lengths of the two sequences it can be estimated that about 20% of the poly(A) containing mRNA molecules could contain one poly(U) sequence. Several lines of evidence have been developed to rule out poly(U) containing heterogeneous nuclear RNA (HnRNA) as the source of the poly(U) sequences in cytoplasm. Poly(U) sequences are also found in RNA molecules of the nucleus and cytoplasm which do not contain poly(A). Although poly(U) sequences are most abundant in nuclear RNA species lacking poly(A), they are well represented in a class of RNA molecules in cytoplasm which lack poly(A), but which otherwise resemble the polysomal mRNA of HeLa cells.", "PMID": 974081}
{"id": "pubmed23n0032_9686", "title": "Chromatographic and functional comparison of human placenta and HeLa cell tyrosine transfer ribonucleic acids.", "content": "Chromatographic and functional properties of tyrosine isoaccepting transfer ribonucleic acids (tRNAs) from placenta and HeLa cells were analyzed and compared. RPC-5 chromatography separated four major isoacceptors from each source, with those from HeLa cells eluting generally later than those from placenta. There was some overlap: HeLa tRNA1Tyr eluted in a position between placenta tRNA3Tyr and tRNA4Tyr; and HeLa tRNA2Tyr and placenta tRNA4Tyr eluted in similar positions with the HeLa isoacceptor eluting slightly later than the placental isoacceptor. Thus there are no isoacceptors common to both sources. The function of the individual isoacceptors was compared in a rabbit-reticulocyte, cell-free, protein-synthesizing system for both the rate of incorporation of tyrosine into the polypeptide chain and the site of incorporation in alpha-globin. Two isoacceptors were compared simultaneously in the same reaction, and overlapping comparisons were made to relate each isoacceptor to all the others. There were no significant differences in the rates of incorporation among the isoacceptors, nor were there any differences in the sites of incorporation. All eight isoacceptors donated tyrosine equally well into the three tyrosine containing tryptic peptides of alpha-globin. Whatever the structural differences among the tyrosine isoacceptors are, they do not affect the function of the tRNA in this protein-synthesizing system.", "contents": "Chromatographic and functional comparison of human placenta and HeLa cell tyrosine transfer ribonucleic acids. Chromatographic and functional properties of tyrosine isoaccepting transfer ribonucleic acids (tRNAs) from placenta and HeLa cells were analyzed and compared. RPC-5 chromatography separated four major isoacceptors from each source, with those from HeLa cells eluting generally later than those from placenta. There was some overlap: HeLa tRNA1Tyr eluted in a position between placenta tRNA3Tyr and tRNA4Tyr; and HeLa tRNA2Tyr and placenta tRNA4Tyr eluted in similar positions with the HeLa isoacceptor eluting slightly later than the placental isoacceptor. Thus there are no isoacceptors common to both sources. The function of the individual isoacceptors was compared in a rabbit-reticulocyte, cell-free, protein-synthesizing system for both the rate of incorporation of tyrosine into the polypeptide chain and the site of incorporation in alpha-globin. Two isoacceptors were compared simultaneously in the same reaction, and overlapping comparisons were made to relate each isoacceptor to all the others. There were no significant differences in the rates of incorporation among the isoacceptors, nor were there any differences in the sites of incorporation. All eight isoacceptors donated tyrosine equally well into the three tyrosine containing tryptic peptides of alpha-globin. Whatever the structural differences among the tyrosine isoacceptors are, they do not affect the function of the tRNA in this protein-synthesizing system.", "PMID": 974082}
{"id": "pubmed23n0032_9687", "title": "Structural difference between alpha-paramyosin and beta-paramyosin of Mercenaria mercenaria.", "content": "A method is described for extraction of alpha-paramyosin in amounts comparable to that formerly attained for beta-paramyosin (15-25 mg/g of muscle). A modification of the procedure for sodium dodecyl sulfate gel electrophoresis is described that permits the separation on coelectrophoresis of alpha-paramyosin (207 000 daltons) and beta-paramyosin (200 000 daltons). The alpha- and beta-paramyosins also can be distinguished by gel electrophoresis at pH 2.3 and by differences in solubility in the region of 0.2-0.4 ionic strength at neutral pH. Evidence is presented that the segment lost from alpha-paramyosin during degradation to beta-paramyosin came from the C-terminal end. This evidence is based on determinations of N- and C-terminal amino acids and on the size of segments obtained after chemical cleavage at the sites of Cys residues. It has been observed earlier that the solubility characteristics of beta-paramyosin at neutral pH are determined by the C-terminal one-third of the molecule and the present results indicate that the additional small segment of about 3.5% of the total mass that is present in the C-terminal end of alpha-paramyosin accounts for the marked difference in solubility of the two forms.", "contents": "Structural difference between alpha-paramyosin and beta-paramyosin of Mercenaria mercenaria. A method is described for extraction of alpha-paramyosin in amounts comparable to that formerly attained for beta-paramyosin (15-25 mg/g of muscle). A modification of the procedure for sodium dodecyl sulfate gel electrophoresis is described that permits the separation on coelectrophoresis of alpha-paramyosin (207 000 daltons) and beta-paramyosin (200 000 daltons). The alpha- and beta-paramyosins also can be distinguished by gel electrophoresis at pH 2.3 and by differences in solubility in the region of 0.2-0.4 ionic strength at neutral pH. Evidence is presented that the segment lost from alpha-paramyosin during degradation to beta-paramyosin came from the C-terminal end. This evidence is based on determinations of N- and C-terminal amino acids and on the size of segments obtained after chemical cleavage at the sites of Cys residues. It has been observed earlier that the solubility characteristics of beta-paramyosin at neutral pH are determined by the C-terminal one-third of the molecule and the present results indicate that the additional small segment of about 3.5% of the total mass that is present in the C-terminal end of alpha-paramyosin accounts for the marked difference in solubility of the two forms.", "PMID": 974083}
{"id": "pubmed23n0032_9688", "title": "beta Structure of aqueous staphylococcal enterotoxin B by spectropolarimetry and sequence-based conformational predictions.", "content": "Conformations of the globular protein staphylococcal enterotoxin B have been examined experimentally by ultraviolet circular dichroism (CD) and visible optical rotatory dispersion (ORD). Chen-Yang-Chau analysis (Chen, Y.-H., Yang, J.T., and Chau, K. H. (1974), Biochemistry 13, 3350) of the far-ultraviolet CD spectrum of native enterotoxin B revealed (assuming an average helix length of 11 residues) 9% alpha helix, 38% beta structure, and 53% random coil. A fourfold increase in alpha-helix was observed for enterotoxin exposed to 0.2% sodium dodecyl sulfate, behavior typical for globular proteins of low helical content. Values of -40 to -50 for the Moffitt-Yang parameter b0 calculated from visible ORD suggested 6-13% alpha helix in native enterotoxin. Application of a new predictive model (Chou, P. Y., and Fasman, G. D. (1974), Biochemistry 13,222) to the amino acid sequence of enterotoxin B indicated 11% alpha helix, 34% beta structure, and 55% coil in native enterotoxin. The excellent agreement for the amount of alpha and beta conformation utilizing different optical and predictive methods indicates beta structure as the dominant secondary structure in native enterotoxin B. Most of the beta structure is predicted by Chou-Fasman analysis to reside in two large regions of antiparallel beta sheet involving residues 81-148 and residues 184-217. Such highly cooperative regions of anti-parallel beta sheet account for the slow unfolding of enterotoxin B in concentrated guanidine hydrochloride and rapid folding of guanidine hydrochloride denatured enterotoxin B to native conformation(s) (Warren, J.R., Spero, L., and Metzger, J. F. (1974), Biochemistry 13, 1678). A more than twofold increase in alpha-helix content with a small diminution in beta structure was detected by CD and ORD upon acidification of aqueous enterotoxin to pH 2.5. Thus, the beta structure of enterotoxin B appears to resist isothermal denaturation and constitutes a stable interior core of structure in the enterotoxin molecule.", "contents": "beta Structure of aqueous staphylococcal enterotoxin B by spectropolarimetry and sequence-based conformational predictions. Conformations of the globular protein staphylococcal enterotoxin B have been examined experimentally by ultraviolet circular dichroism (CD) and visible optical rotatory dispersion (ORD). Chen-Yang-Chau analysis (Chen, Y.-H., Yang, J.T., and Chau, K. H. (1974), Biochemistry 13, 3350) of the far-ultraviolet CD spectrum of native enterotoxin B revealed (assuming an average helix length of 11 residues) 9% alpha helix, 38% beta structure, and 53% random coil. A fourfold increase in alpha-helix was observed for enterotoxin exposed to 0.2% sodium dodecyl sulfate, behavior typical for globular proteins of low helical content. Values of -40 to -50 for the Moffitt-Yang parameter b0 calculated from visible ORD suggested 6-13% alpha helix in native enterotoxin. Application of a new predictive model (Chou, P. Y., and Fasman, G. D. (1974), Biochemistry 13,222) to the amino acid sequence of enterotoxin B indicated 11% alpha helix, 34% beta structure, and 55% coil in native enterotoxin. The excellent agreement for the amount of alpha and beta conformation utilizing different optical and predictive methods indicates beta structure as the dominant secondary structure in native enterotoxin B. Most of the beta structure is predicted by Chou-Fasman analysis to reside in two large regions of antiparallel beta sheet involving residues 81-148 and residues 184-217. Such highly cooperative regions of anti-parallel beta sheet account for the slow unfolding of enterotoxin B in concentrated guanidine hydrochloride and rapid folding of guanidine hydrochloride denatured enterotoxin B to native conformation(s) (Warren, J.R., Spero, L., and Metzger, J. F. (1974), Biochemistry 13, 1678). A more than twofold increase in alpha-helix content with a small diminution in beta structure was detected by CD and ORD upon acidification of aqueous enterotoxin to pH 2.5. Thus, the beta structure of enterotoxin B appears to resist isothermal denaturation and constitutes a stable interior core of structure in the enterotoxin molecule.", "PMID": 974084}
{"id": "pubmed23n0032_9689", "title": "Inactivation of pyridoxal phosphate dependent enzymes by mono- and polyhaloalanines.", "content": "beta,beta-Dichloro- and beta,beta,beta-trifluoroalanine irreversibly inactivate a number of pyridoxal phosphate dependent enzymes which catalyze beta- or gamma-elimination reactions. The inactivation is time dependent and the rate of inactivation is first order in enzyme concentration. This suggests that inactivation is due to covalent modification of the enzyme by a species generated at the active site from the polyhaloalanine (i.e., suicide inactivation). Monohaloalanines are substrates and do not inactivate. For gamma-cystathionase, covalent and stoichiometric attachment of [1-14C]beta,beta,beta-trifluoroalanine was shown. It is proposed that the mechanism of inactivation involves Schiff base formation between inactivator and enzyme-bound pyridoxal and subsequent elimination of HC1 from dichloroalanine or HF from trifluoroalanine. This results in the formation of a beta-halo-alpha,beta unsaturated imine, an activated Michael acceptor. Michael addition of a nucleophile at the active site leads to covalent labeling of the enzyme and inactivation. Alanine racemase is also inactivated by the two polyhaloalanines. Glutamate-pyruvate and gultamate-oxaloacetate transaminase are inactivated by monohaloalanines but not by polyhaloalanines.", "contents": "Inactivation of pyridoxal phosphate dependent enzymes by mono- and polyhaloalanines. beta,beta-Dichloro- and beta,beta,beta-trifluoroalanine irreversibly inactivate a number of pyridoxal phosphate dependent enzymes which catalyze beta- or gamma-elimination reactions. The inactivation is time dependent and the rate of inactivation is first order in enzyme concentration. This suggests that inactivation is due to covalent modification of the enzyme by a species generated at the active site from the polyhaloalanine (i.e., suicide inactivation). Monohaloalanines are substrates and do not inactivate. For gamma-cystathionase, covalent and stoichiometric attachment of [1-14C]beta,beta,beta-trifluoroalanine was shown. It is proposed that the mechanism of inactivation involves Schiff base formation between inactivator and enzyme-bound pyridoxal and subsequent elimination of HC1 from dichloroalanine or HF from trifluoroalanine. This results in the formation of a beta-halo-alpha,beta unsaturated imine, an activated Michael acceptor. Michael addition of a nucleophile at the active site leads to covalent labeling of the enzyme and inactivation. Alanine racemase is also inactivated by the two polyhaloalanines. Glutamate-pyruvate and gultamate-oxaloacetate transaminase are inactivated by monohaloalanines but not by polyhaloalanines.", "PMID": 974085}
{"id": "pubmed23n0032_9690", "title": "Binding of adenosine 5'-diphosphate to creatine kinase. An investigation using intermolecular nuclear Overhauser effect measurements.", "content": "Measurements of the nuclear Overhauser effect (NOE), which is a nuclear magnetic resonance (NMR) double resonance technique, for the H-2 proton on ADP have been used to identify the amino acid residue binding ADP at the active site of creatine kinase. Application of a strong radio-frequency field H2 at a frequency of 0.9 ppm or 1.7 ppm downfield from the proton resonance of 2,2-dimethyl-2-silapentane-5-sulfonate results in a negative NOE for the H2-2 proton resonance of ADP in its complex with creatine kinase. The magnitude of the NOE for the ADPH-2 proton depends on the ratio of ADP to creatine kinase binding site concentration; the dependence indicates that there is rapid exchange between free and bound ADP. Comparable values of the NOE for the H-2 proton of adenosine 5'-mono-, 5'-di-, and 5'-triphosphate and inosine 5'-diphosphate in binary complexes with creatine kinase show that the binding site for these nucleotides is the same. The large negative NOE for the H-2 proton of ADP is maintained for the various binary, ternary, quaternary, and pentenary complexes of creatine kinase with ADP formed by addition of the activator Mg(II), the other substrate creatine, and the planar anion nitrate which is an inhibitor. These results indicate that the conformational changes known to occur upon addition of the other ligands do not involve the entire active site. In particular, the environment around the nucleotide is unperturbed. Inactivation of creatine kinase by reaction with iodoacetamide causes considerable conformational changes. However, as indicated by the large negative NOE for the H-2 proton of ADP in a binary complex with the inactivated enzyme, the environment around the base is altered minimally. Experiments were performed to identify the proton groups on the enzyme, resonating at 0.9 and 1.7 ppm, which interact with the ADPH-2 proton. An NOE was not observed when the aromatic protons of the enzyme were irradiated with the strong radiofrequency field H2 implying that aromatic protons are not near the H-2 proton of ADP in the enzyme complex. The H-2 proton of 1-N6-ethenoadenosine 5'-diphosphate, an analogue of ADP with the 1-nitrogen and 6-nitrogen blocked from potentially hydrogen bonding, still exhibits a large NOE in the nucleotide-enzyme complex. The indication is that the protons promoting the H-2 proton NOE are not on an amino acid residue which binds ADP by hydrogen binding. Creatine kinase was inactivated by reacting the single essential arginyl residue per subunit with diacetyl. No NOE for the H-2 proton of ADP in the presence of the inactivated enzyme was observed. Observation of the H-2 proton resonance of the inhibitor adenosine in the presence of the enzyme revealed no NOE in contrast to the observations with the adenine nucleotides.", "contents": "Binding of adenosine 5'-diphosphate to creatine kinase. An investigation using intermolecular nuclear Overhauser effect measurements. Measurements of the nuclear Overhauser effect (NOE), which is a nuclear magnetic resonance (NMR) double resonance technique, for the H-2 proton on ADP have been used to identify the amino acid residue binding ADP at the active site of creatine kinase. Application of a strong radio-frequency field H2 at a frequency of 0.9 ppm or 1.7 ppm downfield from the proton resonance of 2,2-dimethyl-2-silapentane-5-sulfonate results in a negative NOE for the H2-2 proton resonance of ADP in its complex with creatine kinase. The magnitude of the NOE for the ADPH-2 proton depends on the ratio of ADP to creatine kinase binding site concentration; the dependence indicates that there is rapid exchange between free and bound ADP. Comparable values of the NOE for the H-2 proton of adenosine 5'-mono-, 5'-di-, and 5'-triphosphate and inosine 5'-diphosphate in binary complexes with creatine kinase show that the binding site for these nucleotides is the same. The large negative NOE for the H-2 proton of ADP is maintained for the various binary, ternary, quaternary, and pentenary complexes of creatine kinase with ADP formed by addition of the activator Mg(II), the other substrate creatine, and the planar anion nitrate which is an inhibitor. These results indicate that the conformational changes known to occur upon addition of the other ligands do not involve the entire active site. In particular, the environment around the nucleotide is unperturbed. Inactivation of creatine kinase by reaction with iodoacetamide causes considerable conformational changes. However, as indicated by the large negative NOE for the H-2 proton of ADP in a binary complex with the inactivated enzyme, the environment around the base is altered minimally. Experiments were performed to identify the proton groups on the enzyme, resonating at 0.9 and 1.7 ppm, which interact with the ADPH-2 proton. An NOE was not observed when the aromatic protons of the enzyme were irradiated with the strong radiofrequency field H2 implying that aromatic protons are not near the H-2 proton of ADP in the enzyme complex. The H-2 proton of 1-N6-ethenoadenosine 5'-diphosphate, an analogue of ADP with the 1-nitrogen and 6-nitrogen blocked from potentially hydrogen bonding, still exhibits a large NOE in the nucleotide-enzyme complex. The indication is that the protons promoting the H-2 proton NOE are not on an amino acid residue which binds ADP by hydrogen binding. Creatine kinase was inactivated by reacting the single essential arginyl residue per subunit with diacetyl. No NOE for the H-2 proton of ADP in the presence of the inactivated enzyme was observed. Observation of the H-2 proton resonance of the inhibitor adenosine in the presence of the enzyme revealed no NOE in contrast to the observations with the adenine nucleotides.", "PMID": 974086}
{"id": "pubmed23n0032_9691", "title": "Active-site-directed inactivation of aromatase from human placental microsomes by brominated androgen derivatives.", "content": "Several brominated androgen derivatives were tested for their ability to inactivate microsomal aromatase from term human placenta. In the experimental protocol, the microsomal homogenate was incubated either with androstenedione or a brominated derivative of androstenedione (16alpha-bromo-6-ketoandrostenedione, 16alpha-bromoandrostenedione, 7alpha-(3'-bromoacetoxypropyl)androstenedione, 6alpha-bromoandrostenedione, or 6beta-bromoandrostenedione) and reduced nicotinamide adenine dinucleotide phosphate in a nitrogen saturated buffer composed of glycerol, ethylenediaminetetraacetic acid, and dithiothreitol in tris(hydroxymethyl)aminomethane hydrochloride (pH 7.4) under nitrogen at 4 degrees C with shaking. After the incubation period, the microsomes were recovered by centrifugation and washed once before determining aromatase specific activity. The brominated androgen derivatives which inactivated aromatase were 7alpha-(3'-bromoacetoxypropyl)androstenedione and 6alpha-bromoandrostenedione. The structures of 6alpha- and 6beta-bromoandrostenedione were unequivocally established by single crystal x-ray diffraction techniques. The extent of the enzyme inactivation by 6alpha-bromoandrostenedione was linearly proportional to the logarithm of its concentration. The evidence that this inactivation occurs at the aromatase active site is that androstenedione, when coincubated with 6alpha-bromoandrostenedione, protected aromatase from this inactivation. Progesterone provided much less protection than androstenedione. Furthermore, both 6alpha- and 6beta-bromoandrostenedione are competitive inhibitors of androstenedione aromatization, as determined by a Lineweaver-Burk plot, and 6alpha-bromoandrostenedione gives the same type I cytochrome P-450 binding spectrum with placental microsomes as androstenedione. These data suggest that 6alpha-bromandrostenedione is effective as an active-site-directed inhibitor of placental microsomal aromatase.", "contents": "Active-site-directed inactivation of aromatase from human placental microsomes by brominated androgen derivatives. Several brominated androgen derivatives were tested for their ability to inactivate microsomal aromatase from term human placenta. In the experimental protocol, the microsomal homogenate was incubated either with androstenedione or a brominated derivative of androstenedione (16alpha-bromo-6-ketoandrostenedione, 16alpha-bromoandrostenedione, 7alpha-(3'-bromoacetoxypropyl)androstenedione, 6alpha-bromoandrostenedione, or 6beta-bromoandrostenedione) and reduced nicotinamide adenine dinucleotide phosphate in a nitrogen saturated buffer composed of glycerol, ethylenediaminetetraacetic acid, and dithiothreitol in tris(hydroxymethyl)aminomethane hydrochloride (pH 7.4) under nitrogen at 4 degrees C with shaking. After the incubation period, the microsomes were recovered by centrifugation and washed once before determining aromatase specific activity. The brominated androgen derivatives which inactivated aromatase were 7alpha-(3'-bromoacetoxypropyl)androstenedione and 6alpha-bromoandrostenedione. The structures of 6alpha- and 6beta-bromoandrostenedione were unequivocally established by single crystal x-ray diffraction techniques. The extent of the enzyme inactivation by 6alpha-bromoandrostenedione was linearly proportional to the logarithm of its concentration. The evidence that this inactivation occurs at the aromatase active site is that androstenedione, when coincubated with 6alpha-bromoandrostenedione, protected aromatase from this inactivation. Progesterone provided much less protection than androstenedione. Furthermore, both 6alpha- and 6beta-bromoandrostenedione are competitive inhibitors of androstenedione aromatization, as determined by a Lineweaver-Burk plot, and 6alpha-bromoandrostenedione gives the same type I cytochrome P-450 binding spectrum with placental microsomes as androstenedione. These data suggest that 6alpha-bromandrostenedione is effective as an active-site-directed inhibitor of placental microsomal aromatase.", "PMID": 974087}
{"id": "pubmed23n0032_9692", "title": "Cholesterol exchange between fat cells, chylomicrons and plasma lipoproteins.", "content": "Isolated rat adipocytes were incubated with serum lipoproteins or lymphchylomicrons which contained 14c-labeled cholesterol. The specific activity of lipoprotein free cholesterol decreased and that of cellular free cholesterol increased linearly up to 7 h. At this time the cell cholesterol specific activity was only 11% of that of medium cholesterol indicating that the rate of exchange was slow. The specific activity of lipoprotein esterified cholesterol remained unchanged while that of cells showed a slight increase suggesting esterification of incorporated free cholesterol. No detectable change of the lipoprotein or cellular cholesterol concentration occurred indicating that the uptake of radioactive free cholesterol was due to exchange without net movement of sterol.  The radioactive cholesterol was incorporated into both membrane fraction and the fat droplet of the adipocytes. The rate of cholesterol exchange was temperature-dependent but it was not influenced by the metabolic state of the cells and not by addition of metabolic inhibitors. Trypsin or pronase treatment of the cells were without influence on the rate of the exchange and denaturation of the plasma lipoproteins with formalin increased the rate of exchange. These results indicate that the exchange of cholesterol is a physical chemical process, which is not linked to energy metabolism of the cells, and which is not mediated by either specific lipoprotein receptors on fat cell membranes or pinocytic uptake of lipoproteins. The rate of free cholesterol exchange showed a linear correlation with the concentration of lipoprotein particles in the medium. The relative transfer rate was highest for chylomicrons and decreased in order chylomicron remnants greater than very low density lipoprotein greater than low density lipoprotein greater than high density lipoprotein. A saturation of the system could be obtained only with high density lipoprotein.", "contents": "Cholesterol exchange between fat cells, chylomicrons and plasma lipoproteins. Isolated rat adipocytes were incubated with serum lipoproteins or lymphchylomicrons which contained 14c-labeled cholesterol. The specific activity of lipoprotein free cholesterol decreased and that of cellular free cholesterol increased linearly up to 7 h. At this time the cell cholesterol specific activity was only 11% of that of medium cholesterol indicating that the rate of exchange was slow. The specific activity of lipoprotein esterified cholesterol remained unchanged while that of cells showed a slight increase suggesting esterification of incorporated free cholesterol. No detectable change of the lipoprotein or cellular cholesterol concentration occurred indicating that the uptake of radioactive free cholesterol was due to exchange without net movement of sterol.  The radioactive cholesterol was incorporated into both membrane fraction and the fat droplet of the adipocytes. The rate of cholesterol exchange was temperature-dependent but it was not influenced by the metabolic state of the cells and not by addition of metabolic inhibitors. Trypsin or pronase treatment of the cells were without influence on the rate of the exchange and denaturation of the plasma lipoproteins with formalin increased the rate of exchange. These results indicate that the exchange of cholesterol is a physical chemical process, which is not linked to energy metabolism of the cells, and which is not mediated by either specific lipoprotein receptors on fat cell membranes or pinocytic uptake of lipoproteins. The rate of free cholesterol exchange showed a linear correlation with the concentration of lipoprotein particles in the medium. The relative transfer rate was highest for chylomicrons and decreased in order chylomicron remnants greater than very low density lipoprotein greater than low density lipoprotein greater than high density lipoprotein. A saturation of the system could be obtained only with high density lipoprotein.", "PMID": 974088}
{"id": "pubmed23n0032_9693", "title": "A new rat liver phospholipid exchange protein.", "content": "The soluble fraction from several mammalian tissue homogenates is known to stimulate phospholipid exchange between cell membrane fractions or artificial vesicles. All phospholipid exchange proteins purified to data exhibit an acidic isoelectric point. Using an assay that measures the transfer of [32P] phosphatidylcholine from liposomes to beef heart mitochondria, we report the presence of a new phospholipid exchange protein with a basic isoelectric point (8.4) in rat liver cytosol.  A purification procedure, consisting of pH adjustment to 5.1, gel filtrations on Sephadex G 75 and DE 52 cellulose, isoelectric focusing between a pH of 5 and 10, and gel filtration on Sephadex G-50, yielded a fraction with high phosphatidylcholine exchange activity per mg of protein. This fraction exhibits a major band and two minor bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.  The molecular weight of the major band (18 700) is close to that for basic exchange protein fraction obtained by gel filtration (17 000). The distribution of basic and acidic exchange proteins differs markedly in various tissues and animal species.  About 50 and 35% of phosphatidylcholine exchange activity from rat liver and rat intestine respectively are due to basic phospholipid exchange proteins. In contrast, no basic exchange protein was found in beef heart and only a small amount in beef liver. In the latter organ, less than 10% of phosphatidylcholine exchange activity was due to a basic phospholipid exchange protein fraction.", "contents": "A new rat liver phospholipid exchange protein. The soluble fraction from several mammalian tissue homogenates is known to stimulate phospholipid exchange between cell membrane fractions or artificial vesicles. All phospholipid exchange proteins purified to data exhibit an acidic isoelectric point. Using an assay that measures the transfer of [32P] phosphatidylcholine from liposomes to beef heart mitochondria, we report the presence of a new phospholipid exchange protein with a basic isoelectric point (8.4) in rat liver cytosol.  A purification procedure, consisting of pH adjustment to 5.1, gel filtrations on Sephadex G 75 and DE 52 cellulose, isoelectric focusing between a pH of 5 and 10, and gel filtration on Sephadex G-50, yielded a fraction with high phosphatidylcholine exchange activity per mg of protein. This fraction exhibits a major band and two minor bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.  The molecular weight of the major band (18 700) is close to that for basic exchange protein fraction obtained by gel filtration (17 000). The distribution of basic and acidic exchange proteins differs markedly in various tissues and animal species.  About 50 and 35% of phosphatidylcholine exchange activity from rat liver and rat intestine respectively are due to basic phospholipid exchange proteins. In contrast, no basic exchange protein was found in beef heart and only a small amount in beef liver. In the latter organ, less than 10% of phosphatidylcholine exchange activity was due to a basic phospholipid exchange protein fraction.", "PMID": 974089}
{"id": "pubmed23n0032_9694", "title": "Evidence for carrier proteins in bile acid synthesis. The effect of squalene and sterol carrier protein and albumin on the activity of 12alpha-hydroxylase.", "content": "The possibility that carrier proteins are involved in bile acid synthesis was investigated using rat liver homogenates. The 105 000 X g supernatant fraction was found to contain heat stable proteins that bound the bile acid precursor, 7alpha-hydroxy-4-cholesten-3-one, and increased the amount of 7alpha, 12alpha-dihydroxy-4-cholesten-3-one formed by the microsomal enzyme, 12alpha-hydroxylase. Subsequent studies were carried out to determine if squalene and sterol carrier protein or albumin, two lipid binding proteins present in the 105 00 X g supernatant fraction of rat liver homogenates, may be responsible for the effects seen with this fraction. Squalene and sterol carrier protein bound several water insoluble bile acid precursors, including 7alpha-hydroxy-4-cholesten-3-one, and increased the apparent activity of 12alpha-hydroxylase. Squalene and sterol carrier protein, however, did not bind either cholic acid or chenodeoxycholic acid. Rat serum albumin also bound 7alpha-hydroxy-4-cholesten-3-one and increased the apparent activity of 12alpha-hydroxylase. Kinetic analysis indicated that the apparent stimulation of 12alpha-hydroxylase by squalene and sterol carrier protein and albumin was due to increased solubilization of the substrate, 7alpha-hydroxy-4-cholesten-3-one. Thus, these studies indicate that bile acid precursor carrier proteins are present in the 105 000 Xg supernatant fraction of rat liver homogenates and suggest that squalene and sterol carrier protein or albumin may participate as carrier proteins in bile acid synthesis.", "contents": "Evidence for carrier proteins in bile acid synthesis. The effect of squalene and sterol carrier protein and albumin on the activity of 12alpha-hydroxylase. The possibility that carrier proteins are involved in bile acid synthesis was investigated using rat liver homogenates. The 105 000 X g supernatant fraction was found to contain heat stable proteins that bound the bile acid precursor, 7alpha-hydroxy-4-cholesten-3-one, and increased the amount of 7alpha, 12alpha-dihydroxy-4-cholesten-3-one formed by the microsomal enzyme, 12alpha-hydroxylase. Subsequent studies were carried out to determine if squalene and sterol carrier protein or albumin, two lipid binding proteins present in the 105 00 X g supernatant fraction of rat liver homogenates, may be responsible for the effects seen with this fraction. Squalene and sterol carrier protein bound several water insoluble bile acid precursors, including 7alpha-hydroxy-4-cholesten-3-one, and increased the apparent activity of 12alpha-hydroxylase. Squalene and sterol carrier protein, however, did not bind either cholic acid or chenodeoxycholic acid. Rat serum albumin also bound 7alpha-hydroxy-4-cholesten-3-one and increased the apparent activity of 12alpha-hydroxylase. Kinetic analysis indicated that the apparent stimulation of 12alpha-hydroxylase by squalene and sterol carrier protein and albumin was due to increased solubilization of the substrate, 7alpha-hydroxy-4-cholesten-3-one. Thus, these studies indicate that bile acid precursor carrier proteins are present in the 105 000 Xg supernatant fraction of rat liver homogenates and suggest that squalene and sterol carrier protein or albumin may participate as carrier proteins in bile acid synthesis.", "PMID": 974090}
{"id": "pubmed23n0032_9695", "title": "Phospholipases A1 and A2 in lamellar inclusion bodies of the alveolar epithelium of rabbit lung.", "content": "1. A lamellar body-enriched fraction was prepared from rabbit lung and characterized by electron microscopy, surface activity studies, phospholipid assay and marker enzymes. 2. Both phospholipases A1 and A2 were found to be present in lamellar bodies. After these had been ruptured both enzymes were found to be principally in the soluble phase. 3. The possible roles for phospholipases in lamellar body development and in the respiratory distress syndrome of the newborn are discussed.", "contents": "Phospholipases A1 and A2 in lamellar inclusion bodies of the alveolar epithelium of rabbit lung. 1. A lamellar body-enriched fraction was prepared from rabbit lung and characterized by electron microscopy, surface activity studies, phospholipid assay and marker enzymes. 2. Both phospholipases A1 and A2 were found to be present in lamellar bodies. After these had been ruptured both enzymes were found to be principally in the soluble phase. 3. The possible roles for phospholipases in lamellar body development and in the respiratory distress syndrome of the newborn are discussed.", "PMID": 974092}
{"id": "pubmed23n0032_9696", "title": "Metabolism of different monoacylphospholipids in isolated hepatocytes and the intact rat.", "content": "The 1-[3H] palmitoyl, 2-[3H] oleoyl, and 2-[14C] linoleoyl derivatives of sn-glycero-3-phosphoethanolamine and the corresponding derivatives of sn-glycero-3-phosphocholine were injected intraportally to rats and their incorporation into liver lipids was studied 15 min thereafter. Both the uptake by the liver and the degree of acylation was higher for the unsaturated compounds. The uptake of lysophosphatidylethanolamine was higher than that of lysophosphatidlycholine. The metabolism of 1-lysophosphatidylethanolamine was also studied in isolated hepatocytes. The degree of hydrolysis was much more prominent than in vivo. After injecting 2-[14C] linoleoyl derivatives, a large part of the 14C was recovered in the dienoic phospholipids. Subfractionation by reversed-phase partition chromatography showed that the isotope was located in the palmitoyllinoleoyl and stearoyl-linoleoyl fraction. The 100 X stearoly/(palmitoyl + stearoyl) ratio was 84 in dienoic phosphatidylethanolamine and 59 in dienoic phosphatidylcholine. This preference for stearic acid is significantly larger than in other pathways yielding dienoic phospholipids. It can be concluded that the monoacylphospholipid acyltransferase reactions operating at positions 1 or 2 yield different saturated acyl chain profiles in phosphatidylethanolamine and phosphatidylcholine of a specific unsaturation. This may be important in the regulation of the fatty acid composition of the membrane phospholipids.", "contents": "Metabolism of different monoacylphospholipids in isolated hepatocytes and the intact rat. The 1-[3H] palmitoyl, 2-[3H] oleoyl, and 2-[14C] linoleoyl derivatives of sn-glycero-3-phosphoethanolamine and the corresponding derivatives of sn-glycero-3-phosphocholine were injected intraportally to rats and their incorporation into liver lipids was studied 15 min thereafter. Both the uptake by the liver and the degree of acylation was higher for the unsaturated compounds. The uptake of lysophosphatidylethanolamine was higher than that of lysophosphatidlycholine. The metabolism of 1-lysophosphatidylethanolamine was also studied in isolated hepatocytes. The degree of hydrolysis was much more prominent than in vivo. After injecting 2-[14C] linoleoyl derivatives, a large part of the 14C was recovered in the dienoic phospholipids. Subfractionation by reversed-phase partition chromatography showed that the isotope was located in the palmitoyllinoleoyl and stearoyl-linoleoyl fraction. The 100 X stearoly/(palmitoyl + stearoyl) ratio was 84 in dienoic phosphatidylethanolamine and 59 in dienoic phosphatidylcholine. This preference for stearic acid is significantly larger than in other pathways yielding dienoic phospholipids. It can be concluded that the monoacylphospholipid acyltransferase reactions operating at positions 1 or 2 yield different saturated acyl chain profiles in phosphatidylethanolamine and phosphatidylcholine of a specific unsaturation. This may be important in the regulation of the fatty acid composition of the membrane phospholipids.", "PMID": 974093}
{"id": "pubmed23n0032_9697", "title": "Phospholipid acyl group stability in cultured fibroblasts. Differences between human cell lines of fetal and adult origin.", "content": "Human fibroblasts of both fetal and adult origin incorporated [1-14C] acetate primarily into phospholipid acyl groups (70-80% of total radioactivity). When these labeled cells were replated in non-radioactive medium, there was continuous loss of 14C from steroids, triacylglycerols and non-lipid material. In contrast, after some initial loss, cell lines of fetal origin completely retained 14C in cellular phospholipids during continued cell division. Unlike cells of fetal origin, fibroblasts of adult origin continued to lose radioactivity from their phospholipid acyl groups during growth in unlabeled medium. Loss of radioactivity from [1-3H] acetate incorporated into phospholipids of adult cells cannot be attributed to cell death since it was not accompanied by any loss of previously incorporated [ME-14C] thymidine. If cellular phospholipids were labeled with [U-14C] glycerol, both fetal and adult fibroblasts continued to lose radioisotope from the cells during growth in nonradioactive medium. Thus, there is turnover of the phospholipid molecules themselves in fetal human fibroblasts grown in vitro, but their acyl groups are retained within cellular phospholipids. In this respect, fibroblasts of fetal origin resemble established cell lines such as the L fibroblast. Fibroblasts of adult origin do not exhibit this complete conservation of their phospholipid acyl groups.", "contents": "Phospholipid acyl group stability in cultured fibroblasts. Differences between human cell lines of fetal and adult origin. Human fibroblasts of both fetal and adult origin incorporated [1-14C] acetate primarily into phospholipid acyl groups (70-80% of total radioactivity). When these labeled cells were replated in non-radioactive medium, there was continuous loss of 14C from steroids, triacylglycerols and non-lipid material. In contrast, after some initial loss, cell lines of fetal origin completely retained 14C in cellular phospholipids during continued cell division. Unlike cells of fetal origin, fibroblasts of adult origin continued to lose radioactivity from their phospholipid acyl groups during growth in unlabeled medium. Loss of radioactivity from [1-3H] acetate incorporated into phospholipids of adult cells cannot be attributed to cell death since it was not accompanied by any loss of previously incorporated [ME-14C] thymidine. If cellular phospholipids were labeled with [U-14C] glycerol, both fetal and adult fibroblasts continued to lose radioisotope from the cells during growth in nonradioactive medium. Thus, there is turnover of the phospholipid molecules themselves in fetal human fibroblasts grown in vitro, but their acyl groups are retained within cellular phospholipids. In this respect, fibroblasts of fetal origin resemble established cell lines such as the L fibroblast. Fibroblasts of adult origin do not exhibit this complete conservation of their phospholipid acyl groups.", "PMID": 974094}
{"id": "pubmed23n0032_9698", "title": "Transmission electron microscopy of biliary mixed lipid micelles.", "content": "One potential artifact problem in election microscopy of micellar systems is that structural changes and aggregation could be induced by high vacuum dehydration in sample preparation prior to viewing and photomicrography. This could account for an earlier report on \"macroassemblies\" of biliary lipid, a finding which has been discrepant with the predominance of other evidence on the size, configuration and behavior of biliary micelles. In the present work, this possibility was explored and no evidence for such macroassemblies demonstrated when freeze-drying sample preparation was used; whereas, with conventional technique, the \"macroassemblies\" were again observed. It is concluded that freeze-drying or freeze-etching sample preparation is required to avoid this form of artifact in electron microscopy of micellar systems. The present findings are compatible with a spherical-equivalent or oblate ellipsoid configuration for biliary mixed micelles.", "contents": "Transmission electron microscopy of biliary mixed lipid micelles. One potential artifact problem in election microscopy of micellar systems is that structural changes and aggregation could be induced by high vacuum dehydration in sample preparation prior to viewing and photomicrography. This could account for an earlier report on \"macroassemblies\" of biliary lipid, a finding which has been discrepant with the predominance of other evidence on the size, configuration and behavior of biliary micelles. In the present work, this possibility was explored and no evidence for such macroassemblies demonstrated when freeze-drying sample preparation was used; whereas, with conventional technique, the \"macroassemblies\" were again observed. It is concluded that freeze-drying or freeze-etching sample preparation is required to avoid this form of artifact in electron microscopy of micellar systems. The present findings are compatible with a spherical-equivalent or oblate ellipsoid configuration for biliary mixed micelles.", "PMID": 974095}
{"id": "pubmed23n0032_9699", "title": "Cyclic peroxides and the thiobarbituric assay.", "content": "Several monocyclic peroxide compounds and two acyclic hydroperoxides have been tested for activity in the thiobarbituric acid test. All cyclic peroxides tested which have beta dioxygen functionality on non-tertiary carbons gave positive thiobarbituric acid tests at 532 nm. (epsilon = 10(3) --10(4)) Two acyclic unsaturated hydroperoxides which were derived from gamma-linolenic acid also gave positive thiobarbituric acid tests. In addition to the 532-nm-absorbing species, all peroxidic compounds tested showed a transient absorption at 450 nm during the thiobarbituric acid test. The species responsible for this 450 nm absorption appears to be an intermediate in a series reaction sequence. This intermediate is converted, in time, to the 532-nm-absorbing species. Thiobarbituric acid assay of the crude autoxidation product mixture of gamma-linolenic acid also shows this transient 450-nm-absorbing species. Added ferric ion enhances the 532 nm abosrbance of the thiobarbituric acid assay of cyclic peroxides.", "contents": "Cyclic peroxides and the thiobarbituric assay. Several monocyclic peroxide compounds and two acyclic hydroperoxides have been tested for activity in the thiobarbituric acid test. All cyclic peroxides tested which have beta dioxygen functionality on non-tertiary carbons gave positive thiobarbituric acid tests at 532 nm. (epsilon = 10(3) --10(4)) Two acyclic unsaturated hydroperoxides which were derived from gamma-linolenic acid also gave positive thiobarbituric acid tests. In addition to the 532-nm-absorbing species, all peroxidic compounds tested showed a transient absorption at 450 nm during the thiobarbituric acid test. The species responsible for this 450 nm absorption appears to be an intermediate in a series reaction sequence. This intermediate is converted, in time, to the 532-nm-absorbing species. Thiobarbituric acid assay of the crude autoxidation product mixture of gamma-linolenic acid also shows this transient 450-nm-absorbing species. Added ferric ion enhances the 532 nm abosrbance of the thiobarbituric acid assay of cyclic peroxides.", "PMID": 974096}
{"id": "pubmed23n0032_9700", "title": "Reaction of Tris with aldehydes. Effect of Tris on reactions catalyzed by homoserine dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase.", "content": "Tris buffer was observed to produce an apparent inhibition of the homoserine dehydrogenase (EC 1.1.1.3)-catalyzed reduction of aspartic beta-semialdehyde and an apparent inhibition of the glyceraldehyde phosphate dehydrogenase (EC 1.2.1.9)-catalyzed oxidation of glyceraldehyde 3-phosphate. In each case, the apparent inhibition was found to be due to a lowering of the substrate concentration as a result of a reversible reaction between the free base form of Tris and the substrate, an aldehyde. The product of the reaction was tentatively identified as an imine on the basis of its spectral properties. The inhibition of these two enzymatic reactions by Tris was employed to investigate the kinetics of the reaction of Tris with their substrates. Assuming that these aldehydes exist entirely as the free aldehyde in aqueous solution, equilibrium constants of 369 +/- 12M-1 and 68 +/- 1.5M-1 were determined at 25 degrees C for the reaction of the free base form of Tris with glyceraldehyde 3-phosphate and asparitic beta-semialdehyde, respectively. Correcting for the existence of the hydrated form of glyceraldehyde 3-phosphate in aqueous solution, an equilibrium constant of 1.1-10(4) M-1 was obtained for the reaction of this aldehyde with the free base form of Tris. Forward and reverse direction rate constants for the reaction of Tris with glyceraldehyde 3-phosphate were determined at pH 7.45 and pH 8.5, and both were found to be pH-dependent.", "contents": "Reaction of Tris with aldehydes. Effect of Tris on reactions catalyzed by homoserine dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase. Tris buffer was observed to produce an apparent inhibition of the homoserine dehydrogenase (EC 1.1.1.3)-catalyzed reduction of aspartic beta-semialdehyde and an apparent inhibition of the glyceraldehyde phosphate dehydrogenase (EC 1.2.1.9)-catalyzed oxidation of glyceraldehyde 3-phosphate. In each case, the apparent inhibition was found to be due to a lowering of the substrate concentration as a result of a reversible reaction between the free base form of Tris and the substrate, an aldehyde. The product of the reaction was tentatively identified as an imine on the basis of its spectral properties. The inhibition of these two enzymatic reactions by Tris was employed to investigate the kinetics of the reaction of Tris with their substrates. Assuming that these aldehydes exist entirely as the free aldehyde in aqueous solution, equilibrium constants of 369 +/- 12M-1 and 68 +/- 1.5M-1 were determined at 25 degrees C for the reaction of the free base form of Tris with glyceraldehyde 3-phosphate and asparitic beta-semialdehyde, respectively. Correcting for the existence of the hydrated form of glyceraldehyde 3-phosphate in aqueous solution, an equilibrium constant of 1.1-10(4) M-1 was obtained for the reaction of this aldehyde with the free base form of Tris. Forward and reverse direction rate constants for the reaction of Tris with glyceraldehyde 3-phosphate were determined at pH 7.45 and pH 8.5, and both were found to be pH-dependent.", "PMID": 974097}
{"id": "pubmed23n0032_9701", "title": "Studies on the nature of different molecular forms of glucose-6-phosphate dehydrogenase purified from human leukocytes.", "content": "Several molecular forms of human glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) corresponding to different stages of post-synthetic modifications have been purified from human leukocytes. The various enzyme forms were different in their specific activity, their kinetic properties and their isoelectrofusing pattern. The molecular weight of the subunits of the different forms was not modified. The changes in the electrofocusing pattern were not due to modifications of the N-terminal ends, the oxidation of thiol groups or the non-covalent fixation of an acid molecule upon the enzyme. Carboxypeptidase B cleaved a C-terminal lysine from the different enzyme forms and shifted the isoelectric point of the different enzyme active bands towards the acid pH. The different enzyme forms studied here seemed to result from the action upon 'native glucose-6-phosphate dehydrogenase' of 'modifying factors' especially abundant in some leukemic granulocytes. The modifying factors did not seem to be consumed during the 'modification' of glucose-6-phosphate dehydrogenase. Moreover, the storage for one year of unmodified enzyme resulted in changes in its electrofocusing pattern similar to those quickly induced by the 'modifying factors'. Consequently it appears that the modifying factors are catalysts of the modification of special residues of glucose-6-phosphate dehydrogenase. The hypothesis that this modification involves the deamination of asparagine or glutamine residues is put forward.", "contents": "Studies on the nature of different molecular forms of glucose-6-phosphate dehydrogenase purified from human leukocytes. Several molecular forms of human glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) corresponding to different stages of post-synthetic modifications have been purified from human leukocytes. The various enzyme forms were different in their specific activity, their kinetic properties and their isoelectrofusing pattern. The molecular weight of the subunits of the different forms was not modified. The changes in the electrofocusing pattern were not due to modifications of the N-terminal ends, the oxidation of thiol groups or the non-covalent fixation of an acid molecule upon the enzyme. Carboxypeptidase B cleaved a C-terminal lysine from the different enzyme forms and shifted the isoelectric point of the different enzyme active bands towards the acid pH. The different enzyme forms studied here seemed to result from the action upon 'native glucose-6-phosphate dehydrogenase' of 'modifying factors' especially abundant in some leukemic granulocytes. The modifying factors did not seem to be consumed during the 'modification' of glucose-6-phosphate dehydrogenase. Moreover, the storage for one year of unmodified enzyme resulted in changes in its electrofocusing pattern similar to those quickly induced by the 'modifying factors'. Consequently it appears that the modifying factors are catalysts of the modification of special residues of glucose-6-phosphate dehydrogenase. The hypothesis that this modification involves the deamination of asparagine or glutamine residues is put forward.", "PMID": 974098}
{"id": "pubmed23n0032_9702", "title": "Purification and properties of rat lung soluble glutathione peroxidase.", "content": "Gluthathione peroxidase (gluthatione:hydrogen-peroxide oxidoreductase, EC 1.11.1.9) has been purified approximately 2700-fold from rat lung soluble fraction. The purified enzyme was shown to be homogeneous by sodium dodecyl sulfate/urea polyacrylamide gel electrphoresis. Selenium-75 tracer cochromatographed with the enzyme activity, indicating that rat lung soluble gluthathione peroxidase is a selenium enzyme. The enzyme had an approximate molecular weight of 80000 and contained four identical subunits. The optimal activity of the enzyme was at between pH 8.8 and 9.1. The enzyme had general specificity toward hydroperoxides, and high specificity for reduced glutathione. The kinetic behavior or the purified lung soluble glutathione peroxidase followed a ping-pong-like mechanism; the enzyme first reduced the lipid hydroperoxide substrate to the corresponding hydroxy fatty acid, then was regenerated to the native form by reduced glutathione.", "contents": "Purification and properties of rat lung soluble glutathione peroxidase. Gluthathione peroxidase (gluthatione:hydrogen-peroxide oxidoreductase, EC 1.11.1.9) has been purified approximately 2700-fold from rat lung soluble fraction. The purified enzyme was shown to be homogeneous by sodium dodecyl sulfate/urea polyacrylamide gel electrphoresis. Selenium-75 tracer cochromatographed with the enzyme activity, indicating that rat lung soluble gluthathione peroxidase is a selenium enzyme. The enzyme had an approximate molecular weight of 80000 and contained four identical subunits. The optimal activity of the enzyme was at between pH 8.8 and 9.1. The enzyme had general specificity toward hydroperoxides, and high specificity for reduced glutathione. The kinetic behavior or the purified lung soluble glutathione peroxidase followed a ping-pong-like mechanism; the enzyme first reduced the lipid hydroperoxide substrate to the corresponding hydroxy fatty acid, then was regenerated to the native form by reduced glutathione.", "PMID": 974099}
{"id": "pubmed23n0032_9703", "title": "Transaminase of branched chain amino acids. XI. Leucine (methionine) transaminase of rat liver mitochondria.", "content": "An aminotransferase (transaminase) which is active for leucine and methionine, but not for valine or isoleucine, was purified from rat liver mitochondria. The purified preparation appeared homogeneous on polyacrylamide disc gel electrophoresis. Its molecular weight was shown to be 55 000 by gel filtration. It differed from enzyme II (leucine aminotransferase, EC 2.6.1.6) in the supernatant fraction, another transaminase which is also specific for leucine and methionine, in molecular weight, Km values for substrates, electrophoretic mobility, chromatographic behavior and heat stability. From comparison with related transaminases it was concluded to be a new enzyme and named mitochondrial leucine (methionine) transaminase.", "contents": "Transaminase of branched chain amino acids. XI. Leucine (methionine) transaminase of rat liver mitochondria. An aminotransferase (transaminase) which is active for leucine and methionine, but not for valine or isoleucine, was purified from rat liver mitochondria. The purified preparation appeared homogeneous on polyacrylamide disc gel electrophoresis. Its molecular weight was shown to be 55 000 by gel filtration. It differed from enzyme II (leucine aminotransferase, EC 2.6.1.6) in the supernatant fraction, another transaminase which is also specific for leucine and methionine, in molecular weight, Km values for substrates, electrophoretic mobility, chromatographic behavior and heat stability. From comparison with related transaminases it was concluded to be a new enzyme and named mitochondrial leucine (methionine) transaminase.", "PMID": 974100}
{"id": "pubmed23n0032_9704", "title": "Phosphoglycerate kinase from young and old Turbatrix aceti.", "content": "Phosphoglycerate kinase (ATP:3-phospho-D-glycerate-1-phosphotransferase, EC 2.7.2.3) from young and old Turbatrix aceti has been purified to homogeneity. The \"old\" enzyme exhibits a marked reduction in specific activity both in crude homogenates and in pure form when compared to preparations from young nematodes. The specific activities for pure \"young\" and \"old\" enzymes are 650-750 and 300-400 units/mg, respectively. All other properties of \"young\" and \"old\" enzymes were nearly identical, including molecular weight (43 000), Km, behavior on columns, thermal stability and mobility during gel electrophoresis at three pH values. The results are discussed in terms of the possible mechanism of formation of \"altered\" enzymes. In addition, certain properteis of the nematode phosphoglycerate kinase are compared with those of the enzyme from yeast and rabbit muscle.", "contents": "Phosphoglycerate kinase from young and old Turbatrix aceti. Phosphoglycerate kinase (ATP:3-phospho-D-glycerate-1-phosphotransferase, EC 2.7.2.3) from young and old Turbatrix aceti has been purified to homogeneity. The \"old\" enzyme exhibits a marked reduction in specific activity both in crude homogenates and in pure form when compared to preparations from young nematodes. The specific activities for pure \"young\" and \"old\" enzymes are 650-750 and 300-400 units/mg, respectively. All other properties of \"young\" and \"old\" enzymes were nearly identical, including molecular weight (43 000), Km, behavior on columns, thermal stability and mobility during gel electrophoresis at three pH values. The results are discussed in terms of the possible mechanism of formation of \"altered\" enzymes. In addition, certain properteis of the nematode phosphoglycerate kinase are compared with those of the enzyme from yeast and rabbit muscle.", "PMID": 974101}
{"id": "pubmed23n0032_9705", "title": "Purification and characterization of two forms of urokinase.", "content": "Affinity chromatography on agmatine-Sepharose was used for the separation of two active forms of urokinase (EC 3.4.99.26) from partially purified human urinary urokinase. The approximate molecular weight of the heavier form was 47 000 and of the lighter 33 400. Both forms were homogeneous by sodium dodecyl sulfate gel electrophoresis and by 3H-labeled diisopropylphosphorofluoridate and 14C-labeled p-nitrophenyl-p'-guanidinobenzoate incorporation studies. The 33 400 mol. wt. form had a single chain, and the 47 000 mol. wt. form had two chains (33 100 and 18 600 mol. wt.) linked by disulfide bonds. The specific activity of the heavier form was 104 000 CTA units/mg protein, compared with 226 000 units/mg for the lighter form but the activities per mmol of active site (molar activities) of the two forms were almost identical (9.6-10(9) and 10.2-10(9) CTA units/mmol). Isoelectric focusing on gels showed that the 47 000 material contained one major subform with a pI of 8.60 and a minor subform with a pI of 8.90, while the 33 400 material had three major subforms with pI values of 8.35, 8.60 and 8.70, respectively, and a minor subform with a pI of 8.05. 3H-labeled diisopropylphosphorofluoridate incorporation studies revealed an active-site serine residue in the heavy chain.", "contents": "Purification and characterization of two forms of urokinase. Affinity chromatography on agmatine-Sepharose was used for the separation of two active forms of urokinase (EC 3.4.99.26) from partially purified human urinary urokinase. The approximate molecular weight of the heavier form was 47 000 and of the lighter 33 400. Both forms were homogeneous by sodium dodecyl sulfate gel electrophoresis and by 3H-labeled diisopropylphosphorofluoridate and 14C-labeled p-nitrophenyl-p'-guanidinobenzoate incorporation studies. The 33 400 mol. wt. form had a single chain, and the 47 000 mol. wt. form had two chains (33 100 and 18 600 mol. wt.) linked by disulfide bonds. The specific activity of the heavier form was 104 000 CTA units/mg protein, compared with 226 000 units/mg for the lighter form but the activities per mmol of active site (molar activities) of the two forms were almost identical (9.6-10(9) and 10.2-10(9) CTA units/mmol). Isoelectric focusing on gels showed that the 47 000 material contained one major subform with a pI of 8.60 and a minor subform with a pI of 8.90, while the 33 400 material had three major subforms with pI values of 8.35, 8.60 and 8.70, respectively, and a minor subform with a pI of 8.05. 3H-labeled diisopropylphosphorofluoridate incorporation studies revealed an active-site serine residue in the heavy chain.", "PMID": 974102}
{"id": "pubmed23n0032_9706", "title": "The gamma chain of the lamb.", "content": "The sequence of the gamma-chain of lamb (Finn-Marino) haemoglobin has been investigated. Total amino acid analysis and analysis of the products of cyanylation, together with analysis of tryptic, peptic, thermolysin and Staphylococcus aureus (strain V8) protease peptides and with dansyl-Edman degradation enabled almost the entire sequence to be elucidated. The differences between this sequence and that of the gamma-chains of man, cattle, goat and Rambouillet sheep are discussed.", "contents": "The gamma chain of the lamb. The sequence of the gamma-chain of lamb (Finn-Marino) haemoglobin has been investigated. Total amino acid analysis and analysis of the products of cyanylation, together with analysis of tryptic, peptic, thermolysin and Staphylococcus aureus (strain V8) protease peptides and with dansyl-Edman degradation enabled almost the entire sequence to be elucidated. The differences between this sequence and that of the gamma-chains of man, cattle, goat and Rambouillet sheep are discussed.", "PMID": 974104}
{"id": "pubmed23n0032_9707", "title": "Molecular properties of rat intestinal alkaline phosphatase.", "content": "Rat intestinal alkaline phosphatase is an heterogeneous glycoprotein that contains three protein sub-forms separable by electrophoresis. The molecular weight for the glycoprotein (i.e. the average for the three sub-forms) is 157 000-160 000. Three protein sub-forms are detectable on sodium dodecyl sulphate-polyacrylamide gel electrophoresis that migrate at rates corresponding with molecular weights of 64 000, 79 000 and 92 000. Treatment of native alkaline phosphatase with 6 M guanidine - HC1 or buffer at pH 3.0 results in a product with a molecular weight of 78 000 and 70 000, respectively. Thus it is concluded that each of the three sub-forms is a dimer of identical or closely similar subunits. Limited proteolysis results in the production of new enzymically active sub-forms separable by electrophoresis. Using a bacterial protease it is possible to convert intestinal alkaline phosphatase into a form with a molecular weight of 132 000 without causing any significant change in kinetic properties. Electrophoresis of this new form on sodium dodecyl sulphate polyacrylamide gel suggests that it is composed of 66 000-dalton subunits. The native enzyme contains at least 20% by weight of carbohydrate that probably contributes to microheterogeneity of a second degree superimposed on that stemming from the presence of three protein sub-forms. Treatment with various glycosidases has no effect on electrophoretic behaviour, however. It is suggested that the three sub-forms possibly represent different stages of a maturation process that operates by limited proteolysis of a single parent protein.", "contents": "Molecular properties of rat intestinal alkaline phosphatase. Rat intestinal alkaline phosphatase is an heterogeneous glycoprotein that contains three protein sub-forms separable by electrophoresis. The molecular weight for the glycoprotein (i.e. the average for the three sub-forms) is 157 000-160 000. Three protein sub-forms are detectable on sodium dodecyl sulphate-polyacrylamide gel electrophoresis that migrate at rates corresponding with molecular weights of 64 000, 79 000 and 92 000. Treatment of native alkaline phosphatase with 6 M guanidine - HC1 or buffer at pH 3.0 results in a product with a molecular weight of 78 000 and 70 000, respectively. Thus it is concluded that each of the three sub-forms is a dimer of identical or closely similar subunits. Limited proteolysis results in the production of new enzymically active sub-forms separable by electrophoresis. Using a bacterial protease it is possible to convert intestinal alkaline phosphatase into a form with a molecular weight of 132 000 without causing any significant change in kinetic properties. Electrophoresis of this new form on sodium dodecyl sulphate polyacrylamide gel suggests that it is composed of 66 000-dalton subunits. The native enzyme contains at least 20% by weight of carbohydrate that probably contributes to microheterogeneity of a second degree superimposed on that stemming from the presence of three protein sub-forms. Treatment with various glycosidases has no effect on electrophoretic behaviour, however. It is suggested that the three sub-forms possibly represent different stages of a maturation process that operates by limited proteolysis of a single parent protein.", "PMID": 974105}
{"id": "pubmed23n0032_9708", "title": "Bilirubin and biliverdin binding to rat Y protein (ligandin).", "content": "The binding of bilirubin to poly(L-lysine) produces an optically active complex at pH 10.1. Circular dichroism spectra of these complexes are distinguishable from those generated by binding of bilirubin to the high affinity sites on albumin. Comparison of the circular dichroism spectra of bilirubin bound to the hepatic protein ligandin with those of bilirubin complexed with albumin or polylysine indicates that binding of bilirubin to ligandin occurs at two types of sites. These are distinguishable on the basis of their spectral properties, one resembling the high affinity site of bovine serum albumin and the other resembling polylysine. Complexes of biliverdin with albumin and ligandin bear similarities to the bilirubin-protein complexes. The native protein itself has an ordered structure which consists of 41% alpha-helix and is not altered by the binding of bilirubin.", "contents": "Bilirubin and biliverdin binding to rat Y protein (ligandin). The binding of bilirubin to poly(L-lysine) produces an optically active complex at pH 10.1. Circular dichroism spectra of these complexes are distinguishable from those generated by binding of bilirubin to the high affinity sites on albumin. Comparison of the circular dichroism spectra of bilirubin bound to the hepatic protein ligandin with those of bilirubin complexed with albumin or polylysine indicates that binding of bilirubin to ligandin occurs at two types of sites. These are distinguishable on the basis of their spectral properties, one resembling the high affinity site of bovine serum albumin and the other resembling polylysine. Complexes of biliverdin with albumin and ligandin bear similarities to the bilirubin-protein complexes. The native protein itself has an ordered structure which consists of 41% alpha-helix and is not altered by the binding of bilirubin.", "PMID": 974106}
{"id": "pubmed23n0032_9709", "title": "The binding of 1-anilino-8-naphthalenesulfonate, heparin, salicylate and caprylate by human antithrombin III.", "content": "The binding of 1-anilino-8-naphthalenesulfonate to human antithrombin III was studied by fluorescence enhancement of the fluorophor and fluorescence quenching of the protein emission. Two molecules of 1-anilino-8-naphthalenesulfonate were found to bind per antithrombin molecule with an average dissociation constant of 4.4-10(-5) M. The binding of heparin to antithrombin was studied by ultraviolet difference spectroscopy. The stoichiometry of the heparin binding indicated 1.8 binding sites with an average dissociation constant of 4.3 - 10(-6) M. Further the fluorometric competition experiments with 1-anilino-8-naphthalenesulfonate, heparin, salicylate and caprylate indicated two different classes of anion binding sites on the antithrombin molecule.", "contents": "The binding of 1-anilino-8-naphthalenesulfonate, heparin, salicylate and caprylate by human antithrombin III. The binding of 1-anilino-8-naphthalenesulfonate to human antithrombin III was studied by fluorescence enhancement of the fluorophor and fluorescence quenching of the protein emission. Two molecules of 1-anilino-8-naphthalenesulfonate were found to bind per antithrombin molecule with an average dissociation constant of 4.4-10(-5) M. The binding of heparin to antithrombin was studied by ultraviolet difference spectroscopy. The stoichiometry of the heparin binding indicated 1.8 binding sites with an average dissociation constant of 4.3 - 10(-6) M. Further the fluorometric competition experiments with 1-anilino-8-naphthalenesulfonate, heparin, salicylate and caprylate indicated two different classes of anion binding sites on the antithrombin molecule.", "PMID": 974107}
{"id": "pubmed23n0032_9710", "title": "Changes in the ultraviolet and circular dichroism spectra of aquo-, hydroxy-, azido-, and cyanocobalamin when bound to human intrinsic factor or human transcobalamin I.", "content": "The ultraviolet and the circular dichroic spectra of aquo-, hydroxy-, azido-, and cyanocobalamin free or bound to purified human intrinsic factor or purified human transcobalamin I were recorded. Except for azidocobalamin-transcobalamin I, the molar absorption for the gamma1-band in the ultraviolet spectra increased with a factor between 1.2 and 1.4 when the cobalamins were bound to protein. The ultraviolet spectrum of azidocobalamin-transcobalamin I changed so that the gamma2-band became the most intense. Minor changes were observed in the other ultraviolet spectra. The most prominent change in the CD-spectra of protein bound cobalamins was the increase in negative elipticity above 400 nm. This increase was most intense for cyano- and azidocobalamin-transcobalamin I. The study emphasizes that the mechanism by which human intrinsic factor and human transcobalamin I bind cobalamins differ.", "contents": "Changes in the ultraviolet and circular dichroism spectra of aquo-, hydroxy-, azido-, and cyanocobalamin when bound to human intrinsic factor or human transcobalamin I. The ultraviolet and the circular dichroic spectra of aquo-, hydroxy-, azido-, and cyanocobalamin free or bound to purified human intrinsic factor or purified human transcobalamin I were recorded. Except for azidocobalamin-transcobalamin I, the molar absorption for the gamma1-band in the ultraviolet spectra increased with a factor between 1.2 and 1.4 when the cobalamins were bound to protein. The ultraviolet spectrum of azidocobalamin-transcobalamin I changed so that the gamma2-band became the most intense. Minor changes were observed in the other ultraviolet spectra. The most prominent change in the CD-spectra of protein bound cobalamins was the increase in negative elipticity above 400 nm. This increase was most intense for cyano- and azidocobalamin-transcobalamin I. The study emphasizes that the mechanism by which human intrinsic factor and human transcobalamin I bind cobalamins differ.", "PMID": 974108}
{"id": "pubmed23n0032_9711", "title": "Copper(I) and copper(II) in complexes of biochemical significance studied by X-ray photoelectron spectroscopy.", "content": "X-ray photoelectron spectroscopic measurements of copper complexes of biochemical significance were carried out to permit the conclusion whether or not copper is present in the Cu(I) or Cu(II) state. Only one single homogeneous signal in the X-ray photoelectron spectra of the Cu(I) 2p1/2 and 2p3/2 levels was seen regardless whatever Cu(I) complex was used. By contrast one more or less split satellite in addition to the main 2p copper signal appeared when Cu(II) complexes were studied. The extent of satellite splitting was dependent on the nature of the ligands coordinated with Cu(II). Thus, a strong splitting was observed in the spectra of Cu-(trifluoroacetylacetonate)2 and Cu-(biuret)2Cl2 were Cu(II) is exclusively bound to oxygen having a formal double bond. No such splitting was seen in Cu(II) chelates where the metal was bound to single bonded oxygen and/or nitrogen. It excited great interest to see that in the antiferromagnetically coupled Cu(II) complexes Cu2-(succinate)2-4H2O, Cu-(HCCO)2, CuO and in the completely diamagnetic Cu2-(u,3-diphenyltriazene)4 complex Cu(II) could be detected. The reaction of Cu(I) and Cu(II) with the thiol sulphur of either cysteine, penicillamine or alpha-mercaptopropionylglycine yielded Cu(I) complexes. During the X-ray exposure of the different samples photoreduction of Cu(II) was not observed. For example, the satellite structure of the copper 2p levels using Cu(II)-cystine remained unchanged during the measurement.", "contents": "Copper(I) and copper(II) in complexes of biochemical significance studied by X-ray photoelectron spectroscopy. X-ray photoelectron spectroscopic measurements of copper complexes of biochemical significance were carried out to permit the conclusion whether or not copper is present in the Cu(I) or Cu(II) state. Only one single homogeneous signal in the X-ray photoelectron spectra of the Cu(I) 2p1/2 and 2p3/2 levels was seen regardless whatever Cu(I) complex was used. By contrast one more or less split satellite in addition to the main 2p copper signal appeared when Cu(II) complexes were studied. The extent of satellite splitting was dependent on the nature of the ligands coordinated with Cu(II). Thus, a strong splitting was observed in the spectra of Cu-(trifluoroacetylacetonate)2 and Cu-(biuret)2Cl2 were Cu(II) is exclusively bound to oxygen having a formal double bond. No such splitting was seen in Cu(II) chelates where the metal was bound to single bonded oxygen and/or nitrogen. It excited great interest to see that in the antiferromagnetically coupled Cu(II) complexes Cu2-(succinate)2-4H2O, Cu-(HCCO)2, CuO and in the completely diamagnetic Cu2-(u,3-diphenyltriazene)4 complex Cu(II) could be detected. The reaction of Cu(I) and Cu(II) with the thiol sulphur of either cysteine, penicillamine or alpha-mercaptopropionylglycine yielded Cu(I) complexes. During the X-ray exposure of the different samples photoreduction of Cu(II) was not observed. For example, the satellite structure of the copper 2p levels using Cu(II)-cystine remained unchanged during the measurement.", "PMID": 974109}
{"id": "pubmed23n0032_9712", "title": "Fluorescence study of N-(3-pyrene)maleimide conjugated to rabbit skeletal F-actin and plasmodium actin polymers.", "content": "A fluorescent probe N-(3-pyrene)maleimide was conjugated to rabbit skeletal F-actin at the site of most reactive sulfhydryl group (Cys-373). Its fluorescence anisotropy decay showed a single correlation time of 560 ns at 25 degrees C, which is in a very good agreement with the correlation time of the dansyl-L-cysteine group conjugated to the same site of F-actin reported very recently [Wahl, Ph., Mihashi, K, and Auchet, J-C. (1975) FEBS Lett. 8, 164-167]. Actin from plasmodia of myxomycates, Physarum polycepharum, was also conjugated with N-(3-pyrene) maleimide and the fluorescence anisotropy was compared with rabbit skeletal F-actin using the classical steady excitation method. It was found that the internal mobility of the magnesium polymer of plasmodium actin is remarkably larger than both plasmodium F-actin and rabbit skeletal F-actin.", "contents": "Fluorescence study of N-(3-pyrene)maleimide conjugated to rabbit skeletal F-actin and plasmodium actin polymers. A fluorescent probe N-(3-pyrene)maleimide was conjugated to rabbit skeletal F-actin at the site of most reactive sulfhydryl group (Cys-373). Its fluorescence anisotropy decay showed a single correlation time of 560 ns at 25 degrees C, which is in a very good agreement with the correlation time of the dansyl-L-cysteine group conjugated to the same site of F-actin reported very recently [Wahl, Ph., Mihashi, K, and Auchet, J-C. (1975) FEBS Lett. 8, 164-167]. Actin from plasmodia of myxomycates, Physarum polycepharum, was also conjugated with N-(3-pyrene) maleimide and the fluorescence anisotropy was compared with rabbit skeletal F-actin using the classical steady excitation method. It was found that the internal mobility of the magnesium polymer of plasmodium actin is remarkably larger than both plasmodium F-actin and rabbit skeletal F-actin.", "PMID": 974110}
{"id": "pubmed23n0032_9713", "title": "Protein fluorescence and electronic energy transfer in the determination of molecular dimensions and rotational relaxation times of native and coenzyme-bound horse liver alcohol dehydrogenase.", "content": "The intrinsic fluorescence lifetimes of horse liver alcohol dehydrogenase (EC 1.1.1.1) and pig heart isocitrate dehydrogenase (EC 1.1.1.42) have been determined to be 5.36 ns and 4.84 ns, respectively. When reduced coenzyme is bound, the fluorescence lifetime of alcohol dehydrogenase is reduced to 4.98 ns while that of isocitrate dehydrogenase remains unchanged. Oxidized coenzymes have no effect on fluorescence lifetimes of alcohol and isocitrate dehydrogenases. This virtual constancy of protein fluorescence lifetimes has allowed the conclusion to be reached that in protein-ligand complexes with equilibrium constants in the range of 10(4)-10(6) M(-1), the static mode of quenching is substantial. The observation of resonance energy transfer in alcohol dehydrogenase-NADH complex facilitates the determination of the distance between tryptophan and the reduced nicotinamide ring involved in the transfer as 30.6 A, compared to the effective molecular radius of 36.2 A for alcohol dehydrogenase. The increased rotational relaxation times of coenzyme-bound alcohol dehydrogenase relative to the unliganded form (sigmah = 72 ns) indicate in this protein structural fluctuations occurring in the time range of nanoseconds.", "contents": "Protein fluorescence and electronic energy transfer in the determination of molecular dimensions and rotational relaxation times of native and coenzyme-bound horse liver alcohol dehydrogenase. The intrinsic fluorescence lifetimes of horse liver alcohol dehydrogenase (EC 1.1.1.1) and pig heart isocitrate dehydrogenase (EC 1.1.1.42) have been determined to be 5.36 ns and 4.84 ns, respectively. When reduced coenzyme is bound, the fluorescence lifetime of alcohol dehydrogenase is reduced to 4.98 ns while that of isocitrate dehydrogenase remains unchanged. Oxidized coenzymes have no effect on fluorescence lifetimes of alcohol and isocitrate dehydrogenases. This virtual constancy of protein fluorescence lifetimes has allowed the conclusion to be reached that in protein-ligand complexes with equilibrium constants in the range of 10(4)-10(6) M(-1), the static mode of quenching is substantial. The observation of resonance energy transfer in alcohol dehydrogenase-NADH complex facilitates the determination of the distance between tryptophan and the reduced nicotinamide ring involved in the transfer as 30.6 A, compared to the effective molecular radius of 36.2 A for alcohol dehydrogenase. The increased rotational relaxation times of coenzyme-bound alcohol dehydrogenase relative to the unliganded form (sigmah = 72 ns) indicate in this protein structural fluctuations occurring in the time range of nanoseconds.", "PMID": 974111}
{"id": "pubmed23n0032_9714", "title": "Factors affecting the luminescence of tryptophan at 77 K.", "content": "The effects of solvent polarity, heavy atoms and sulphur-containing compounds on the fluorescence and phosphorescence of indole and tryptophan have been investigated. The low-temperature luminescence of a group of dipeptides containing tryptophan is also reported. The phosphorescence to fluorescence quantum yield ratio and the phosphorescence lifetime are shown to be particularly sensitive to environmental factors. The relevance of these results to the interpretation of protein luminescence spectra is briefly discussed.", "contents": "Factors affecting the luminescence of tryptophan at 77 K. The effects of solvent polarity, heavy atoms and sulphur-containing compounds on the fluorescence and phosphorescence of indole and tryptophan have been investigated. The low-temperature luminescence of a group of dipeptides containing tryptophan is also reported. The phosphorescence to fluorescence quantum yield ratio and the phosphorescence lifetime are shown to be particularly sensitive to environmental factors. The relevance of these results to the interpretation of protein luminescence spectra is briefly discussed.", "PMID": 974112}
{"id": "pubmed23n0032_9715", "title": "The effect of trypsin on bovine transferrin and lactoferrin.", "content": "The iron-saturated and iron-free (apo) forms of bovine transferrin and lactoferrin were digested with trypsin and the digests analysed by column chromatography and electrophoresis. Both of the iron-saturated proteins were more resistant to proteolysis than the corresponding apoproteins, and iron-transferrin was more resistant than iron-lactoferrin. Digestion of iron-transferrin yielded two iron-binding fragments with molecular weights of 32 000 and 38 500 whereas apotransferrin yielded only the larger fragment. In digests of lactoferrin, up to five different fragments with molecular weights ranging from 25 000 to 52 700 were detected, there being no obvious qualitative difference between digests of iron-lactoferrin and apolactoferrin. The susceptibility of apolactoferrin to tryptic digestion was only slightly reduced when apolactoferrin was complexed with beta-lactoglobulin, suggesting that complex-formation is not a mechanism for protecting lactoferrin against intestinal degradation. There was no immunological cross reaction between bovine transferrin or its digestion products against anti-lactoferrin antiserum, or vice-versa.", "contents": "The effect of trypsin on bovine transferrin and lactoferrin. The iron-saturated and iron-free (apo) forms of bovine transferrin and lactoferrin were digested with trypsin and the digests analysed by column chromatography and electrophoresis. Both of the iron-saturated proteins were more resistant to proteolysis than the corresponding apoproteins, and iron-transferrin was more resistant than iron-lactoferrin. Digestion of iron-transferrin yielded two iron-binding fragments with molecular weights of 32 000 and 38 500 whereas apotransferrin yielded only the larger fragment. In digests of lactoferrin, up to five different fragments with molecular weights ranging from 25 000 to 52 700 were detected, there being no obvious qualitative difference between digests of iron-lactoferrin and apolactoferrin. The susceptibility of apolactoferrin to tryptic digestion was only slightly reduced when apolactoferrin was complexed with beta-lactoglobulin, suggesting that complex-formation is not a mechanism for protecting lactoferrin against intestinal degradation. There was no immunological cross reaction between bovine transferrin or its digestion products against anti-lactoferrin antiserum, or vice-versa.", "PMID": 974113}
{"id": "pubmed23n0032_9716", "title": "Studies on the sulfhydryl groups in type III collagen.", "content": "The Type III collagen molecule, [alpha](III)]3, is comprised of three alphal(III) chains each of which contains two cysteinyl residues. Free sulfhydryl groups, however, could not be detected in the denatured, trimeric gamma-component of Type III collagen as judged by the failure to form derivatives with the alkylating reagents iodo-[14C]acetic acid and 4-vinylpyridine. This absence of sulfhydryl group reactivity did not appear to result from disulfide-binding between Type III collagen and noncollagenous peptides. Collectively, the results indicate that all of the six sulfhydryl groups of the Type III collagen molecule participate in interchain disulfide-bonding.", "contents": "Studies on the sulfhydryl groups in type III collagen. The Type III collagen molecule, [alpha](III)]3, is comprised of three alphal(III) chains each of which contains two cysteinyl residues. Free sulfhydryl groups, however, could not be detected in the denatured, trimeric gamma-component of Type III collagen as judged by the failure to form derivatives with the alkylating reagents iodo-[14C]acetic acid and 4-vinylpyridine. This absence of sulfhydryl group reactivity did not appear to result from disulfide-binding between Type III collagen and noncollagenous peptides. Collectively, the results indicate that all of the six sulfhydryl groups of the Type III collagen molecule participate in interchain disulfide-bonding.", "PMID": 974114}
{"id": "pubmed23n0032_9717", "title": "One electron reduction of metmyoglobin and methemoglobin and the reaction of the reduced molecule with oxygen.", "content": "We have used the pulse radiolysis technique to reduce with solvated electrons (see article) a single Fe(III) site in methemoglobin and metmyoglobin. The reduction process was followed spectrophotometrically and the reactions rate constants were measured: (see article) =6.5 +/- 1-10(10) M-1-S-1. (see article)=2.5 +/- 0.3-10(10) M-1-S-1. Approx. 60% of the (see article) have reacted with the hemin group, and the rest of the (see article) have probably reacted with the globin moiety. We followed the reaction of the reduced proteins to yield the oxyderivatives and measured the rate constants of the oxygenation process k reduced methemoglobin + O2 = 2.6 +/- 0.6-10(7) M-1-S-1 and k myoglobin + O2 = 1.8 +/- 0.2-10(7) M-1-S-1, all the rate constants were measured at pH = 6.8, I = 0.004, T = 22 +/- 2 degrees C. The high rate constant for reduced methemoglobin indicates that one-site-reduced methemoglobin is probably in the R state, as predicted for methemoglobin from X-ray analysis. The spectra of the reduced and oxygenated species were measured under similar conditions at gamma = 450-650 nm. We were able to follow slight changes in the micro-second time scale, these changes were attributed to conformational changes. We were not able to detect any reaction between the radical (see article) and the hemin group (which would result in a complex such as heme O-2). This may be due to kinetic reasons.", "contents": "One electron reduction of metmyoglobin and methemoglobin and the reaction of the reduced molecule with oxygen. We have used the pulse radiolysis technique to reduce with solvated electrons (see article) a single Fe(III) site in methemoglobin and metmyoglobin. The reduction process was followed spectrophotometrically and the reactions rate constants were measured: (see article) =6.5 +/- 1-10(10) M-1-S-1. (see article)=2.5 +/- 0.3-10(10) M-1-S-1. Approx. 60% of the (see article) have reacted with the hemin group, and the rest of the (see article) have probably reacted with the globin moiety. We followed the reaction of the reduced proteins to yield the oxyderivatives and measured the rate constants of the oxygenation process k reduced methemoglobin + O2 = 2.6 +/- 0.6-10(7) M-1-S-1 and k myoglobin + O2 = 1.8 +/- 0.2-10(7) M-1-S-1, all the rate constants were measured at pH = 6.8, I = 0.004, T = 22 +/- 2 degrees C. The high rate constant for reduced methemoglobin indicates that one-site-reduced methemoglobin is probably in the R state, as predicted for methemoglobin from X-ray analysis. The spectra of the reduced and oxygenated species were measured under similar conditions at gamma = 450-650 nm. We were able to follow slight changes in the micro-second time scale, these changes were attributed to conformational changes. We were not able to detect any reaction between the radical (see article) and the hemin group (which would result in a complex such as heme O-2). This may be due to kinetic reasons.", "PMID": 974115}
{"id": "pubmed23n0032_9718", "title": "Renaturation of formyltetrahydrofolate synthetase from urea and guanidinium chloride solutions.", "content": "Formyltetrahydrofolate synthetase from Clostridium cylindrosporum and Clostridium acidi-urici was denatured in 6 M urea and 4 M guanidinium chloride. Viscometric, fluorimetric and ultracentrifugal measurements were used to determine that the protein is completely unfolded under these conditions. The polypeptide chains refold upon dilution of the denaturant-protein solutions to give final concentrations of 0.5 M urea or 0.1 M guanidinium chloride. In the presence of NH4+, but not in its absence, the refolded proteins associate to produce the catalytically active tetramer. Refolding and reassociation were followed by measuring changes in protein fluorescence and by determination of sedimentation constants. Under most conditions 80% of the enzymic activity is recovered.", "contents": "Renaturation of formyltetrahydrofolate synthetase from urea and guanidinium chloride solutions. Formyltetrahydrofolate synthetase from Clostridium cylindrosporum and Clostridium acidi-urici was denatured in 6 M urea and 4 M guanidinium chloride. Viscometric, fluorimetric and ultracentrifugal measurements were used to determine that the protein is completely unfolded under these conditions. The polypeptide chains refold upon dilution of the denaturant-protein solutions to give final concentrations of 0.5 M urea or 0.1 M guanidinium chloride. In the presence of NH4+, but not in its absence, the refolded proteins associate to produce the catalytically active tetramer. Refolding and reassociation were followed by measuring changes in protein fluorescence and by determination of sedimentation constants. Under most conditions 80% of the enzymic activity is recovered.", "PMID": 974116}
{"id": "pubmed23n0032_9719", "title": "Isolement et caract\u00e9risation de deux subunites constitutives des glycoproteines de structure du tissu sous cutan\u00e9 de lapin.", "content": "Structural glycoproteins have been extracted by 8 M ureau from the insoluble residue remaining after collagenase digestion of rabbit dermis and purified by Sepharose 4 B chromatography. After reduction and alkylation, Dowex 1 x 2 chromatography allowed separation of two structural glycoproteins (D1 and D2) in an homogenous state as shown by chromatographic and electrophoretic behaviour as well as N terminal amino acid determination. These two glycoproteins have a molecular weight of about 16 000. Their amino acid compositions (very similar), are characterized by a high level of dicarboxylic amino acids and the absence of hydroxyproline and hydroxylysine. The less acidic glycoprotein (D1) has glycine for N terminal amino acid and contains 10.4 percent of bound carbohydrates. The glycoprotein D2 contains 5.1 percent of bound carbohydrates and its N terminal amino acid is glutamic acid.", "contents": "Isolement et caract\u00e9risation de deux subunites constitutives des glycoproteines de structure du tissu sous cutan\u00e9 de lapin. Structural glycoproteins have been extracted by 8 M ureau from the insoluble residue remaining after collagenase digestion of rabbit dermis and purified by Sepharose 4 B chromatography. After reduction and alkylation, Dowex 1 x 2 chromatography allowed separation of two structural glycoproteins (D1 and D2) in an homogenous state as shown by chromatographic and electrophoretic behaviour as well as N terminal amino acid determination. These two glycoproteins have a molecular weight of about 16 000. Their amino acid compositions (very similar), are characterized by a high level of dicarboxylic amino acids and the absence of hydroxyproline and hydroxylysine. The less acidic glycoprotein (D1) has glycine for N terminal amino acid and contains 10.4 percent of bound carbohydrates. The glycoprotein D2 contains 5.1 percent of bound carbohydrates and its N terminal amino acid is glutamic acid.", "PMID": 974117}
{"id": "pubmed23n0032_9720", "title": "Covalently closed minicircular DNA associated with Acetabularia chloroplasts.", "content": "When Acetabularia cliftonii chloroplast DNA (p = 1.706 g/cm3) is centrifuged in an ethidium bromide-CsCl gradient, the lower band is enriched for DNA with a buoyant density of 1.712 g/cm3 containing small covalently closed circular molecules. The minicircles measure 4.15 +/- 0.30 mum in the closed conformation and 4.35 +/- 0.20 mum in the open conformation. They are not of nuclear or bacterial origin, and appear to exist as independent entities within the chloroplast, although a mitochondrial origin cannot be completely ruled out. No 40-45 mum circles, as found in other chloroplasts, were found in either ethidium bromide-CsCl fraction. None were found in total chloroplast DNA by any of a number of methods tried. Linear molecules up to 200 mum were measured in chloroplast lysates. The main chloroplast genome may consist of very large circular molecules which are broken by even small shear forces.", "contents": "Covalently closed minicircular DNA associated with Acetabularia chloroplasts. When Acetabularia cliftonii chloroplast DNA (p = 1.706 g/cm3) is centrifuged in an ethidium bromide-CsCl gradient, the lower band is enriched for DNA with a buoyant density of 1.712 g/cm3 containing small covalently closed circular molecules. The minicircles measure 4.15 +/- 0.30 mum in the closed conformation and 4.35 +/- 0.20 mum in the open conformation. They are not of nuclear or bacterial origin, and appear to exist as independent entities within the chloroplast, although a mitochondrial origin cannot be completely ruled out. No 40-45 mum circles, as found in other chloroplasts, were found in either ethidium bromide-CsCl fraction. None were found in total chloroplast DNA by any of a number of methods tried. Linear molecules up to 200 mum were measured in chloroplast lysates. The main chloroplast genome may consist of very large circular molecules which are broken by even small shear forces.", "PMID": 974121}
{"id": "pubmed23n0032_9721", "title": "Control of transfer RNA synthesis in the presence of inhibitors of protein synthesis.", "content": "The rate of synthesis of transfer RNA in suspension cultures of Chang's liver cells, has been examined in the presence of various inhibitors of protein synthesis with different modes of action. Inhibitors of polypeptide chain elongation such as cycloheximide and emetine stimulated the rate of synthesis of transfer RNA at concentrations that inhibited protein synthesis by 60-90%. Trichodermin, an inhibitor of the elongation and termination steps in protein synthesis, had as effect similar to that of cycloheximide and emetine. On the other hand verrucarin, an inhibitor of initiation, and puromycin, an analogue of the aminoacyl terminus of tRNA, had little effect of the synthesis of transfer RNA at low concentrations. At high concentrations these compounds inhibited transfer RNA synthesis. Inhibitors of protein synthesis can be divided in two groups based on their effect on the polysom pattern. The first group increased the number of large polysomes, while verrucarin and puromycin lead to a break down of large polysomes and to an accumulation of monosomes and small polysomes. Thus, there appears to be a correlation between the effect of these inhibitors of protein synthesis on the aggregational state of the polysomes and their effect on the rate of transfer RNA synthesis.", "contents": "Control of transfer RNA synthesis in the presence of inhibitors of protein synthesis. The rate of synthesis of transfer RNA in suspension cultures of Chang's liver cells, has been examined in the presence of various inhibitors of protein synthesis with different modes of action. Inhibitors of polypeptide chain elongation such as cycloheximide and emetine stimulated the rate of synthesis of transfer RNA at concentrations that inhibited protein synthesis by 60-90%. Trichodermin, an inhibitor of the elongation and termination steps in protein synthesis, had as effect similar to that of cycloheximide and emetine. On the other hand verrucarin, an inhibitor of initiation, and puromycin, an analogue of the aminoacyl terminus of tRNA, had little effect of the synthesis of transfer RNA at low concentrations. At high concentrations these compounds inhibited transfer RNA synthesis. Inhibitors of protein synthesis can be divided in two groups based on their effect on the polysom pattern. The first group increased the number of large polysomes, while verrucarin and puromycin lead to a break down of large polysomes and to an accumulation of monosomes and small polysomes. Thus, there appears to be a correlation between the effect of these inhibitors of protein synthesis on the aggregational state of the polysomes and their effect on the rate of transfer RNA synthesis.", "PMID": 974122}
{"id": "pubmed23n0032_9722", "title": "The structural organization of nuclear messenger RNA precursor. I. Reassociation and hybridization properties of double-stranded hairpin-like loops in messenger RNA precursor.", "content": "The hybridization and renaturation properties of double-stranded hairpin-like loops isolated from giant nuclear messenger RNA precursor of mouse liver or ascites carcinoma cells were studied. About half of the hairpins in messenger RNA precursor appear to contain similar sequences, as indicated by the very fast kinetics of renaturation of the denatured double-stranded RNA sequences. These sequences have no tissue specificity. About one third of the hairpin sequences can hybridize to messenger RNA. It is suggested that the long hairpins in messenger RNA precursor play the role of sequences separating messenger RNA sequences from non-informative sequences and that these hairpins are recognized by processing enzymes.", "contents": "The structural organization of nuclear messenger RNA precursor. I. Reassociation and hybridization properties of double-stranded hairpin-like loops in messenger RNA precursor. The hybridization and renaturation properties of double-stranded hairpin-like loops isolated from giant nuclear messenger RNA precursor of mouse liver or ascites carcinoma cells were studied. About half of the hairpins in messenger RNA precursor appear to contain similar sequences, as indicated by the very fast kinetics of renaturation of the denatured double-stranded RNA sequences. These sequences have no tissue specificity. About one third of the hairpin sequences can hybridize to messenger RNA. It is suggested that the long hairpins in messenger RNA precursor play the role of sequences separating messenger RNA sequences from non-informative sequences and that these hairpins are recognized by processing enzymes.", "PMID": 974123}
{"id": "pubmed23n0032_9723", "title": "Dielectric increment of low molecular weight RNA in dioxane after application of static electric fields.", "content": "A soluble RNA with a molecular weight between 15 000 and 23 000 was extracted from calf thymus and chromatographically purified. The RNA was slightly soluble in doxane (approx. 2.5 mug/ml) and RNA-dioxane solutions were used to fill a cell (452.02 pF capacitance) to which static electric fields of variable strength were applied. The dielectric permittivity of the solutions was measured at a fixed time interval after the application of the electric field. The RNA solutions showed a dielectric increment proportional to the strength of the applied field and to the RNA concentration. The existence of a hystersis effect was proven and the effect of the electric field on the RNA molecule might be related to a dielectric saturation phenomenon parallel to long-term changes of the molecule.", "contents": "Dielectric increment of low molecular weight RNA in dioxane after application of static electric fields. A soluble RNA with a molecular weight between 15 000 and 23 000 was extracted from calf thymus and chromatographically purified. The RNA was slightly soluble in doxane (approx. 2.5 mug/ml) and RNA-dioxane solutions were used to fill a cell (452.02 pF capacitance) to which static electric fields of variable strength were applied. The dielectric permittivity of the solutions was measured at a fixed time interval after the application of the electric field. The RNA solutions showed a dielectric increment proportional to the strength of the applied field and to the RNA concentration. The existence of a hystersis effect was proven and the effect of the electric field on the RNA molecule might be related to a dielectric saturation phenomenon parallel to long-term changes of the molecule.", "PMID": 974124}
{"id": "pubmed23n0032_9724", "title": "Permanganate ion oxidations. IX. Manganese intermediates (complexes) in the oxidation of 2,4(1H,3H)-pyrimidinediones.", "content": "Uniquely stable manganese intermediates (complexes) are formed from the permanganate ion oxidation of the 5,6-carbon-carbon double bond in several 2,4(1H,3H)-pyrimidinediones [uracil, (compound 7), 5-methyluracil (thymine, compound 5), and 6-methyluracil (compound 8)]. These manganese complexes, which represent some of the most stable intermediate manganese species observed thus far in the oxidation of carbon-carbon double bonds, show absorption maxima in the 285-296 nm region (epsilon max approximately 4500). The relative reactivities of 6-methyluracil: uracil: thymine are 1: 23 : 194 and the bimolecular oxidation process is characterized by relatively small deltaH++ values and large negative deltaS++ values.", "contents": "Permanganate ion oxidations. IX. Manganese intermediates (complexes) in the oxidation of 2,4(1H,3H)-pyrimidinediones. Uniquely stable manganese intermediates (complexes) are formed from the permanganate ion oxidation of the 5,6-carbon-carbon double bond in several 2,4(1H,3H)-pyrimidinediones [uracil, (compound 7), 5-methyluracil (thymine, compound 5), and 6-methyluracil (compound 8)]. These manganese complexes, which represent some of the most stable intermediate manganese species observed thus far in the oxidation of carbon-carbon double bonds, show absorption maxima in the 285-296 nm region (epsilon max approximately 4500). The relative reactivities of 6-methyluracil: uracil: thymine are 1: 23 : 194 and the bimolecular oxidation process is characterized by relatively small deltaH++ values and large negative deltaS++ values.", "PMID": 974125}
{"id": "pubmed23n0032_9725", "title": "Fluorescent labeling of DNA in solution with covalently bound acriflavin.", "content": "We have prepared a fluorescent derivative of DNA based on the acriflavin-Feulgen histological procedure for staining DNA. Our procedure involved binding acriflavin to DNA in solution by reacting the acriflavin with aldehydes formed on the deoxyribose of DNA by controlled removal of a few percent of the purine bases of the DNA. Partially depurinated DNA was reacted with the acriflavin reagent, and unbound acriflavin was removed by chromatography on Sephadex G-25 eluted with phosphate buffered guanidine -HCl. Such single-stranded depurinated DNA bound 0.36 acriflavin molecules per 100 purine bases per h of depurination. DNA containing one bound acriflavin per 200 bases reassociated at 85% of the value of control DNA. The acriflavin - DNA complex showed new absorption maxima at 466 and 370 nm. The fluorescent product had excitation maxima at 304 and 465 nm and an emission maximum at 502 nm. This labeling procedure should be useful in place of or in addition to radioactive labeling for DNA.", "contents": "Fluorescent labeling of DNA in solution with covalently bound acriflavin. We have prepared a fluorescent derivative of DNA based on the acriflavin-Feulgen histological procedure for staining DNA. Our procedure involved binding acriflavin to DNA in solution by reacting the acriflavin with aldehydes formed on the deoxyribose of DNA by controlled removal of a few percent of the purine bases of the DNA. Partially depurinated DNA was reacted with the acriflavin reagent, and unbound acriflavin was removed by chromatography on Sephadex G-25 eluted with phosphate buffered guanidine -HCl. Such single-stranded depurinated DNA bound 0.36 acriflavin molecules per 100 purine bases per h of depurination. DNA containing one bound acriflavin per 200 bases reassociated at 85% of the value of control DNA. The acriflavin - DNA complex showed new absorption maxima at 466 and 370 nm. The fluorescent product had excitation maxima at 304 and 465 nm and an emission maximum at 502 nm. This labeling procedure should be useful in place of or in addition to radioactive labeling for DNA.", "PMID": 974126}
{"id": "pubmed23n0032_9726", "title": "Mechanism of hydroxylamine mutagenesis. Crystal structure and conformation of 1,5-dimethyl-N4-hydroxycytosine.", "content": "The crystal structure of the title compound, which is a formal analogue of 5-methyl-N4-hydroxycytosine nucleosides, has been determined by X-ray diffraction. The space group is P2(1)/c with a = 7.368 (2), b = 12.096 (3), c = 9.192 (4) A, beta = 113.94 (3) degrees. Three-dimensional intensity data were collected with a four-circle diffractometer, and the structure was refined by block-diagonal least-squares to R = 0.053. The compound is in the imino form, and the exocyclic N4-OH is located essentially in the plane of the pyrimidine ring, and syn to the ring (N(3). There is an intramolecular hydrogen bond involving the N(3)-H as donor and O(4) as acceptor, viz. N(3)-H(31)----O(4)-H. With this conformation, which probably prevails also in solution, the compound would be unable to participate in normal Watson-Crick base pairing. It is shown that a similar situation may prevail for N4-hydroxycytosine nucleosides. The implications with regard to the molecular mechanism of hydroxylamine mutagenesis, with particular reference to the T-even bacteriophages, are discussed. Analogous considerations are applied to an examination of the possible behaviour of hydroxylamine-modified adenine nucleosides.", "contents": "Mechanism of hydroxylamine mutagenesis. Crystal structure and conformation of 1,5-dimethyl-N4-hydroxycytosine. The crystal structure of the title compound, which is a formal analogue of 5-methyl-N4-hydroxycytosine nucleosides, has been determined by X-ray diffraction. The space group is P2(1)/c with a = 7.368 (2), b = 12.096 (3), c = 9.192 (4) A, beta = 113.94 (3) degrees. Three-dimensional intensity data were collected with a four-circle diffractometer, and the structure was refined by block-diagonal least-squares to R = 0.053. The compound is in the imino form, and the exocyclic N4-OH is located essentially in the plane of the pyrimidine ring, and syn to the ring (N(3). There is an intramolecular hydrogen bond involving the N(3)-H as donor and O(4) as acceptor, viz. N(3)-H(31)----O(4)-H. With this conformation, which probably prevails also in solution, the compound would be unable to participate in normal Watson-Crick base pairing. It is shown that a similar situation may prevail for N4-hydroxycytosine nucleosides. The implications with regard to the molecular mechanism of hydroxylamine mutagenesis, with particular reference to the T-even bacteriophages, are discussed. Analogous considerations are applied to an examination of the possible behaviour of hydroxylamine-modified adenine nucleosides.", "PMID": 974127}
{"id": "pubmed23n0032_9727", "title": "Effect of starvation of the distribution of free and membrane-bound ribosomes in rat liver and on the content of phospholipid and glycogen in purified ribosomes.", "content": "The distribution of free and membrane-bound ribosomes in liver in response to starvation has not been clearly defined. An investigation has been made of the effects of starvation on the content of DNA, RNA, protein, phospholipid and glycogen in rat liver, on the distribution of free and membrane-bound ribosomes, and on the content of phospholipid and glycogen in free and bound ribosome fractions. The results indicate that starvation can produce up to a 50% reduction in hepatic ribosomes without altering either the fraction of rRNA relative to the total RNA or the distribution of free and membrane-bound ribosomes. In addition, the degree of contamination of isolated ribosomes with membranous material does not fluctuate with changes in the nutritional status of the animal. The results suggest that the relative capacities for protein synthesis among the two ribosome compartments are maintained during the early stages of starvation. Further, co-sedimentation with glycogen is not responsible for the presence of membranous materials in purified ribosomes.", "contents": "Effect of starvation of the distribution of free and membrane-bound ribosomes in rat liver and on the content of phospholipid and glycogen in purified ribosomes. The distribution of free and membrane-bound ribosomes in liver in response to starvation has not been clearly defined. An investigation has been made of the effects of starvation on the content of DNA, RNA, protein, phospholipid and glycogen in rat liver, on the distribution of free and membrane-bound ribosomes, and on the content of phospholipid and glycogen in free and bound ribosome fractions. The results indicate that starvation can produce up to a 50% reduction in hepatic ribosomes without altering either the fraction of rRNA relative to the total RNA or the distribution of free and membrane-bound ribosomes. In addition, the degree of contamination of isolated ribosomes with membranous material does not fluctuate with changes in the nutritional status of the animal. The results suggest that the relative capacities for protein synthesis among the two ribosome compartments are maintained during the early stages of starvation. Further, co-sedimentation with glycogen is not responsible for the presence of membranous materials in purified ribosomes.", "PMID": 974128}
{"id": "pubmed23n0032_9728", "title": "Characterization of DNA polymerase associated with nuclear membrane fractions from uninfected and adenovirus 2-infected KB cells.", "content": "We have isolated a nuclear membrane fraction from KB cells infected with human adenovirus 2 that synthesizes exclusively small viral DNA chains (approx. 9 S) in vitro (Yamashita, T., Arens, M. and Green, M. (1975) J. Biol. Chem. 250, 3273-3279). The DNA polymerase activity present in the adenovirus 2 DNA-nuclear membrane complex was purified through chromatography on phosphocellulose and DEAE-cellulose, glycerol gradient centrifuation and DNA-cellulose chromatography. A single peak of enzymatic activity sedimented in glycerol gradients at about 6.7 S which corresponds to a molecular weight of 125000. The enzyme preparation in the step of glycerol gradient centrifugation utilized activated calf thymus, KB cell and adenovirus 2 DNA as template-primer in the presence of Mg2+; Km values for these DNAs were 5.5, 4.0, and 0.8 mug/ml, respectively. The partially purified enzyme preparation was characterized by several criteria which were compared to the properties of the three major mammalian DNA polymerases, alpha, beta, and psi. On the basis of template-primer preference, effect of salt, inhibition by N-ethylmaleimide and Km for dTTP, the DNA polymerase activity from the membrane complex can be distinguished from the alpha and beta DNA polymerases. The elution profile from DNA cellulose revealed a minor peak (I) and a major peak (II) of DNA polymerase activity utilizing poly(A) -(dT)10 as template-primer in the presence of Mn2+ - Peak II from DNA cellulose, which contained about 90% of the total DNA polymerase activity eluted from the column, was 2-3 times as active with poly(A) - (dT)10 as template-primer in the presence of Mn2+ than with activated calf thymus DNA in the presence of Mg2+. On the other hand, peak I had a low ratio of poly(A) - (dT)10 to activated calf thymus DNA activity. DNA polymerase was also purified from the nuclear membrane fraction of uninfected KB cells by the same procedures as those used in enzyme purification from the adenovirus 2 DNA-nuclear membrane complex. A minor peak and a major peak of DNA polymerase activity utilizing poly(A) - (dT)10 as template primer in the presence of Mn2+ were again observed that eluted from DNA cellulose at the same KCl concentrations as peak I and II from adenovirus 2-infected cells. The enzymes of the nuclear membrane fraction of uninfected KB cells could not be differentiated from the enzymes of the adenovirus 2 DNA-nuclear membrane complex through any of the purification steps nor by their template specificities. These results indicate that the predominant enzyme in the adenovirus 2 DNA-nuclear membrane complex and in the KB cell nuclear membrane complex belongs to the class of DNA polymerase psi.", "contents": "Characterization of DNA polymerase associated with nuclear membrane fractions from uninfected and adenovirus 2-infected KB cells. We have isolated a nuclear membrane fraction from KB cells infected with human adenovirus 2 that synthesizes exclusively small viral DNA chains (approx. 9 S) in vitro (Yamashita, T., Arens, M. and Green, M. (1975) J. Biol. Chem. 250, 3273-3279). The DNA polymerase activity present in the adenovirus 2 DNA-nuclear membrane complex was purified through chromatography on phosphocellulose and DEAE-cellulose, glycerol gradient centrifuation and DNA-cellulose chromatography. A single peak of enzymatic activity sedimented in glycerol gradients at about 6.7 S which corresponds to a molecular weight of 125000. The enzyme preparation in the step of glycerol gradient centrifugation utilized activated calf thymus, KB cell and adenovirus 2 DNA as template-primer in the presence of Mg2+; Km values for these DNAs were 5.5, 4.0, and 0.8 mug/ml, respectively. The partially purified enzyme preparation was characterized by several criteria which were compared to the properties of the three major mammalian DNA polymerases, alpha, beta, and psi. On the basis of template-primer preference, effect of salt, inhibition by N-ethylmaleimide and Km for dTTP, the DNA polymerase activity from the membrane complex can be distinguished from the alpha and beta DNA polymerases. The elution profile from DNA cellulose revealed a minor peak (I) and a major peak (II) of DNA polymerase activity utilizing poly(A) -(dT)10 as template-primer in the presence of Mn2+ - Peak II from DNA cellulose, which contained about 90% of the total DNA polymerase activity eluted from the column, was 2-3 times as active with poly(A) - (dT)10 as template-primer in the presence of Mn2+ than with activated calf thymus DNA in the presence of Mg2+. On the other hand, peak I had a low ratio of poly(A) - (dT)10 to activated calf thymus DNA activity. DNA polymerase was also purified from the nuclear membrane fraction of uninfected KB cells by the same procedures as those used in enzyme purification from the adenovirus 2 DNA-nuclear membrane complex. A minor peak and a major peak of DNA polymerase activity utilizing poly(A) - (dT)10 as template primer in the presence of Mn2+ were again observed that eluted from DNA cellulose at the same KCl concentrations as peak I and II from adenovirus 2-infected cells. The enzymes of the nuclear membrane fraction of uninfected KB cells could not be differentiated from the enzymes of the adenovirus 2 DNA-nuclear membrane complex through any of the purification steps nor by their template specificities. These results indicate that the predominant enzyme in the adenovirus 2 DNA-nuclear membrane complex and in the KB cell nuclear membrane complex belongs to the class of DNA polymerase psi.", "PMID": 974129}
{"id": "pubmed23n0032_9729", "title": "Comparison of the temperature sensitivity of protein synthesis by cell-free systems from liver of rat and skate (Raja ocellata).", "content": "Studies were undertaken to determine the component(s) responsible for the temperature optimum characteristic of the protein-synthesizing system from skate and rat. 1. The macromolecular constituents of rat and skate liver ribosomes are compared. The number of ribosomal proteins is similar in the two species, although most proteins display different electrophoretic mobilities on polyacrylamide gels. The RNAs from the small subunit of skate and rat have similar sedimentation coefficients; however, the RNA from the large subunit of skate ribosomes appeared to be slight smaller than the comparable RNA from the rat. 2. Ribosomes from either rat or skate were capable of supporting poly(U)dependent polyphenylalanine synthesis with soluble factors from either species. 3. Maximal leucine incorporation directed by endogenous mRNA occurred at 35--40 degrees C with post-mitochondrial supernatant from the rat liver and at 20--30 degrees C with that from skate liver. 4. The characteristic temperature sensitivity of protein synthesis was dependent upon the source of cell sap and independent of the source of ribosomes. 5. Elongation factor 1 from both the rat and skate exhibited maximum activity at approx. 30 degrees C. 6. Phenylalanyl-tRNA synthetase from skate liver showed maximum activity at 30 degrees C while that from rat was maximally active at 37 degrees C. The rat enzyme, however, was active at 0--10 degrees C, at which temperature protein synthesis in the reconstructed rat system is virtually absent. 7. The protein-synthesizing capacity of the reconstituted system at various temperatures was closely correlated with the activity of Elongation factor 2 (translocase). Elongation factor 2 from rat liver displayed an optimum at 30 degrees C and lost all activity below 10 degrees C, while this same factor from skate liver showed an optimum at 20 degrees C and significant activity below 10 degrees C. At this low temperature the reconstituted skate liver system continued to exhibit the ability to synthesize protein. These studies suggest that Elongation factor 2 is the component responsible for determining the temperature at which the protein-synthesizing system displays its characteristic maximum activity.", "contents": "Comparison of the temperature sensitivity of protein synthesis by cell-free systems from liver of rat and skate (Raja ocellata). Studies were undertaken to determine the component(s) responsible for the temperature optimum characteristic of the protein-synthesizing system from skate and rat. 1. The macromolecular constituents of rat and skate liver ribosomes are compared. The number of ribosomal proteins is similar in the two species, although most proteins display different electrophoretic mobilities on polyacrylamide gels. The RNAs from the small subunit of skate and rat have similar sedimentation coefficients; however, the RNA from the large subunit of skate ribosomes appeared to be slight smaller than the comparable RNA from the rat. 2. Ribosomes from either rat or skate were capable of supporting poly(U)dependent polyphenylalanine synthesis with soluble factors from either species. 3. Maximal leucine incorporation directed by endogenous mRNA occurred at 35--40 degrees C with post-mitochondrial supernatant from the rat liver and at 20--30 degrees C with that from skate liver. 4. The characteristic temperature sensitivity of protein synthesis was dependent upon the source of cell sap and independent of the source of ribosomes. 5. Elongation factor 1 from both the rat and skate exhibited maximum activity at approx. 30 degrees C. 6. Phenylalanyl-tRNA synthetase from skate liver showed maximum activity at 30 degrees C while that from rat was maximally active at 37 degrees C. The rat enzyme, however, was active at 0--10 degrees C, at which temperature protein synthesis in the reconstructed rat system is virtually absent. 7. The protein-synthesizing capacity of the reconstituted system at various temperatures was closely correlated with the activity of Elongation factor 2 (translocase). Elongation factor 2 from rat liver displayed an optimum at 30 degrees C and lost all activity below 10 degrees C, while this same factor from skate liver showed an optimum at 20 degrees C and significant activity below 10 degrees C. At this low temperature the reconstituted skate liver system continued to exhibit the ability to synthesize protein. These studies suggest that Elongation factor 2 is the component responsible for determining the temperature at which the protein-synthesizing system displays its characteristic maximum activity.", "PMID": 974130}
{"id": "pubmed23n0032_9730", "title": "Excision of ultraviolet and gamma ray products of the 5,6-dihydroxy-dihydrothymine type by nuclear preperations of xeroderma pigmentosum cells.", "content": "Monomeric products of the 5,6-dihydroxy-dihydrothymine type are produced in the DNA by both ultraviolet and ionizing radiations. The capacity of nuclear preparations from normal and Xeroderma pigmentosum (XP) cells (Complementation groups A, B, C and D) to excise such products from ultraviolet or gamma-irradiated T7DNA was comparable and was independent of radiation induced strand breaks.", "contents": "Excision of ultraviolet and gamma ray products of the 5,6-dihydroxy-dihydrothymine type by nuclear preperations of xeroderma pigmentosum cells. Monomeric products of the 5,6-dihydroxy-dihydrothymine type are produced in the DNA by both ultraviolet and ionizing radiations. The capacity of nuclear preparations from normal and Xeroderma pigmentosum (XP) cells (Complementation groups A, B, C and D) to excise such products from ultraviolet or gamma-irradiated T7DNA was comparable and was independent of radiation induced strand breaks.", "PMID": 974131}
{"id": "pubmed23n0032_9731", "title": "The crystal and molecular structure of the polymeric complex of zinc(II) withcytosine 5'-phosphate: metal bonding to both N(3) and phosphate.", "content": "Crystal structure analysis of the zinc(II) complex with cytosine 5'-phosphate, (C9H12N3O8P)Zn(H2O), shows that the complex is two-dimensionally polymeric, and the zinc atom is tetrahedrally coordinated to N(3) of the pyrimidine, to two phosphate oxygen atoms and to one water molecule. The zinc atom also forms weak intramolecular interaction with O(2) of the pyrimidine.", "contents": "The crystal and molecular structure of the polymeric complex of zinc(II) withcytosine 5'-phosphate: metal bonding to both N(3) and phosphate. Crystal structure analysis of the zinc(II) complex with cytosine 5'-phosphate, (C9H12N3O8P)Zn(H2O), shows that the complex is two-dimensionally polymeric, and the zinc atom is tetrahedrally coordinated to N(3) of the pyrimidine, to two phosphate oxygen atoms and to one water molecule. The zinc atom also forms weak intramolecular interaction with O(2) of the pyrimidine.", "PMID": 974132}
{"id": "pubmed23n0032_9732", "title": "Giant RNA in insects. I. Differential changes during Calliphora development and the role of ecdysone.", "content": "The pattern of newly synthesized RNA in the epidermis cells of Calliphora during the larval life to the early pupal stage was analysed by electrophoretic separation of RNA extracts on polyacrylamide gels. During larval life, mainly rRNA was synthesized. The elevation of ecdysone concentration during the synthesis of the new larval cuticle resulted primarily in the stimulation of rRNA synthesis. In the transition period from larva to pupa, the principal RNA synthesized was rapidly labelled giant DNA-like RNA, with an average life span of 30 min. Prior to the change in RNA metabolism at this development stage, the synthesis of all classes of RNA was depressed. The initial increase in hnRNA synthesis occurs at the onset of the wandering stage, shortly after the appearance of a small peak of ecdysone and about two days prior to the main peak of hormone secretion and pupariation. During induced pupariation by ecdysterone, 1 h after hormone treatment, more label was observed in giant hnRNA followed by elevation of rRNA synthesis. Processing and maturation of rRNA also seemed to be stimulated.", "contents": "Giant RNA in insects. I. Differential changes during Calliphora development and the role of ecdysone. The pattern of newly synthesized RNA in the epidermis cells of Calliphora during the larval life to the early pupal stage was analysed by electrophoretic separation of RNA extracts on polyacrylamide gels. During larval life, mainly rRNA was synthesized. The elevation of ecdysone concentration during the synthesis of the new larval cuticle resulted primarily in the stimulation of rRNA synthesis. In the transition period from larva to pupa, the principal RNA synthesized was rapidly labelled giant DNA-like RNA, with an average life span of 30 min. Prior to the change in RNA metabolism at this development stage, the synthesis of all classes of RNA was depressed. The initial increase in hnRNA synthesis occurs at the onset of the wandering stage, shortly after the appearance of a small peak of ecdysone and about two days prior to the main peak of hormone secretion and pupariation. During induced pupariation by ecdysterone, 1 h after hormone treatment, more label was observed in giant hnRNA followed by elevation of rRNA synthesis. Processing and maturation of rRNA also seemed to be stimulated.", "PMID": 974134}
{"id": "pubmed23n0032_9733", "title": "Stimulation of cell proliferation in rat liver by alpha-hexachlorocyclohexane or partial hepatectomy and end points during G1 of the inhibitory action of beta-diethyl-aminoethylphenyldiallyl acetate-HC1 (CFT 1201), beta-diethylaminoethyldiphenylpropyl acetate. HC1 (SKF 525-A) and actinomycin D.", "content": "The present work was designed to study the nature, sequence and temporal position of some inhibitor-sensitive events of the replicative cycle in rat liver. Hepatocyte proliferation was induced by alpha-hexachlorocyclohexane and by partial hepatectomy; the onset of DNA synthesis and of mitotic activity were determined and used as reference points in the cell cycle. Inhibition of cell proliferation was achieved by CFT 1201, SKF 525-A, and actinomycin D. It was found that the inhibitory action of the three agents ends at the same stage of the replicative cycle, 0--2 h before the G1/S transition, in both alpha-hexachlorocyclohexane-stimulated and regenerating rat liver. It is concluded that the molecular events sensitive to CFT 1201, SKF 525-A or actinomycin D are either identical or temporally closely associated; they do not figure in the metabolic activation of alpha-hexachlorocyclohexane.", "contents": "Stimulation of cell proliferation in rat liver by alpha-hexachlorocyclohexane or partial hepatectomy and end points during G1 of the inhibitory action of beta-diethyl-aminoethylphenyldiallyl acetate-HC1 (CFT 1201), beta-diethylaminoethyldiphenylpropyl acetate. HC1 (SKF 525-A) and actinomycin D. The present work was designed to study the nature, sequence and temporal position of some inhibitor-sensitive events of the replicative cycle in rat liver. Hepatocyte proliferation was induced by alpha-hexachlorocyclohexane and by partial hepatectomy; the onset of DNA synthesis and of mitotic activity were determined and used as reference points in the cell cycle. Inhibition of cell proliferation was achieved by CFT 1201, SKF 525-A, and actinomycin D. It was found that the inhibitory action of the three agents ends at the same stage of the replicative cycle, 0--2 h before the G1/S transition, in both alpha-hexachlorocyclohexane-stimulated and regenerating rat liver. It is concluded that the molecular events sensitive to CFT 1201, SKF 525-A or actinomycin D are either identical or temporally closely associated; they do not figure in the metabolic activation of alpha-hexachlorocyclohexane.", "PMID": 974135}
{"id": "pubmed23n0032_9734", "title": "Incorporation of L-azetidine-2-carboxylic acid into hemoglobin S in sickle erythrocytes in vitro.", "content": "L-Azetidine-2-carboxylic acid, the naturally occurring lower homologue of L-proline, is incorporated into hemoglobin S (sickle hemoglobin) in vitro. Sickle erythrocytes from patients with sickle cell anemia incubated with L-[3H] azetidine-2-carboxylate synthesized radiolabeled hemoglobin which when isolated from cell lysates, co-chromatographed with hemoglobin S on DEAE-cellulose columns. The alpha/beta ratio of azetidine carboxylate incorporation into the globin chains of sickle hemoglobin was 0.94, which is consistent with the presence of four proline residues in each polypeptide chain. Incorporation of azetidine carboxylate into hot trichloroacetic acid-insoluble material in sickle erythrocytes indicated that the homologue was present in the polypeptide backbone of the globin chains of sickle hemoglobin. Amino acid analysis of the hot trichloroacetic acid-insoluble material from sickle erythrocytes which had been incubated with radiolabeled azetidine carboxylate indicated that 75% of the radioactivity could be accounted for as intact homologue while 20% of the radioactivity co-chromatographed with alanine. These results suggest that azetidine carboxylate is incorporated unaltered into hemoglobin S in addition to being metabolized to alanine in sickle erythrocytes prior to incorporation into protein. The kinetics of thermal precipitation of hemoglobin S (oxygen ligand) into which radioactive azetidine carboxylate or radioactive proline had been incorporated in vitro is identical. This observation, together with the behavior of hemoglobin S and the globin chains from hemoglobin S containing azetidine carboxylate during ion-exchange chromatography, indicates that homologue replacement of prolyl residues does not significantly alter the overall charge or stability of the hemoglobin S tetramer. Azetidine carboxylate did not inhibit uptake of radiolabeled proline by sickle erythrocytes suggesting that the homologue does not adversely affect amino acid transport in these cells.", "contents": "Incorporation of L-azetidine-2-carboxylic acid into hemoglobin S in sickle erythrocytes in vitro. L-Azetidine-2-carboxylic acid, the naturally occurring lower homologue of L-proline, is incorporated into hemoglobin S (sickle hemoglobin) in vitro. Sickle erythrocytes from patients with sickle cell anemia incubated with L-[3H] azetidine-2-carboxylate synthesized radiolabeled hemoglobin which when isolated from cell lysates, co-chromatographed with hemoglobin S on DEAE-cellulose columns. The alpha/beta ratio of azetidine carboxylate incorporation into the globin chains of sickle hemoglobin was 0.94, which is consistent with the presence of four proline residues in each polypeptide chain. Incorporation of azetidine carboxylate into hot trichloroacetic acid-insoluble material in sickle erythrocytes indicated that the homologue was present in the polypeptide backbone of the globin chains of sickle hemoglobin. Amino acid analysis of the hot trichloroacetic acid-insoluble material from sickle erythrocytes which had been incubated with radiolabeled azetidine carboxylate indicated that 75% of the radioactivity could be accounted for as intact homologue while 20% of the radioactivity co-chromatographed with alanine. These results suggest that azetidine carboxylate is incorporated unaltered into hemoglobin S in addition to being metabolized to alanine in sickle erythrocytes prior to incorporation into protein. The kinetics of thermal precipitation of hemoglobin S (oxygen ligand) into which radioactive azetidine carboxylate or radioactive proline had been incorporated in vitro is identical. This observation, together with the behavior of hemoglobin S and the globin chains from hemoglobin S containing azetidine carboxylate during ion-exchange chromatography, indicates that homologue replacement of prolyl residues does not significantly alter the overall charge or stability of the hemoglobin S tetramer. Azetidine carboxylate did not inhibit uptake of radiolabeled proline by sickle erythrocytes suggesting that the homologue does not adversely affect amino acid transport in these cells.", "PMID": 974136}
{"id": "pubmed23n0032_9735", "title": "Control of protein synthesis by hemin. Isolation and characterization of a supernatant factor from rabbit reticulocyte lysate.", "content": "The regulation of protein synthesis by hemin in rabbit reticulocyte lysates is mediated by a hemin-controlled translational repressor protein (HCR) that inhibits polypeptide chain initiation. The effect of this translational inhibitor can be reversed by a high molecular weight protein in the post-ribosomal supernatant fraction. This supernatant factor has been purified approx. 700-fold. It is as effective in reversing the inhibition of protein synthesis due to an early form of HCR (intermediate HCR) as it is in stimulating protein synthesis in the absence of hemin. It is progressively less effective at reversing the inhibition of protein synthesis due to a late from of HCR (irreversible HCR), double-stranded RNA, and oxidized glutathione. The supernatant factor is chromatographically different from the initiation factor IF-MP, isolated from reticulocyte ribosomes, that can also overcome the inhibitory effect of HCR. The supernatant factor does not require hemin for activity, and its action is somewhat suppressed by a level of hemin that is optimal for protein synthesis.", "contents": "Control of protein synthesis by hemin. Isolation and characterization of a supernatant factor from rabbit reticulocyte lysate. The regulation of protein synthesis by hemin in rabbit reticulocyte lysates is mediated by a hemin-controlled translational repressor protein (HCR) that inhibits polypeptide chain initiation. The effect of this translational inhibitor can be reversed by a high molecular weight protein in the post-ribosomal supernatant fraction. This supernatant factor has been purified approx. 700-fold. It is as effective in reversing the inhibition of protein synthesis due to an early form of HCR (intermediate HCR) as it is in stimulating protein synthesis in the absence of hemin. It is progressively less effective at reversing the inhibition of protein synthesis due to a late from of HCR (irreversible HCR), double-stranded RNA, and oxidized glutathione. The supernatant factor is chromatographically different from the initiation factor IF-MP, isolated from reticulocyte ribosomes, that can also overcome the inhibitory effect of HCR. The supernatant factor does not require hemin for activity, and its action is somewhat suppressed by a level of hemin that is optimal for protein synthesis.", "PMID": 974137}
{"id": "pubmed23n0032_9736", "title": "Different susceptibility of protein synthesis to inhibitors of elongation in cell-free systems from plasma cell tumours and reticulocytes.", "content": "Plasma cells and reticulocytes are mammalian cell systems which have specialized in the synthesis of a single protein during their differentiation from one common stem cell. To study whether there is a difference in cell susceptibility at the level of elongation, dose vs. inhibition curves of sparsomycin, cycloheximide and emetine in cell-free systems with S-30 fractions from plasma cell tumours (MOPC 63, MOPC 41, RPC 20, MOPC 104 E), reticulocytes and liver were compared. The experiments revealed: (1) all the selected systems are equally sensitive to sparsomycin; (2) the susceptibility of the reticulocyte systems to cycloheximide and emetine is higher than that of the plasma cell tumours. In the dose range of 1 - 10(-7) --5 - 10(-5) M cycloheximide and 1 - 10(-6)--1 - 10(-4) M emetine the reticulocyte system is preferentially inhibited; (3) the sensitivities of all plasma cell tumours are equal; (4) the liver system is more sensitive to emetine than to cycloheximide; (5) the site of the different susceptibility to these antibiotics could be located on the ribosomes; (6) however, when the extracts of the plasma cell tumours were prepared in the presence of hemin, their susceptibility rises and is like that of reticulocytes. These results show that hemin promotes in a cell-specific manner the sensitivity to some inhibitors of protein synthesis.", "contents": "Different susceptibility of protein synthesis to inhibitors of elongation in cell-free systems from plasma cell tumours and reticulocytes. Plasma cells and reticulocytes are mammalian cell systems which have specialized in the synthesis of a single protein during their differentiation from one common stem cell. To study whether there is a difference in cell susceptibility at the level of elongation, dose vs. inhibition curves of sparsomycin, cycloheximide and emetine in cell-free systems with S-30 fractions from plasma cell tumours (MOPC 63, MOPC 41, RPC 20, MOPC 104 E), reticulocytes and liver were compared. The experiments revealed: (1) all the selected systems are equally sensitive to sparsomycin; (2) the susceptibility of the reticulocyte systems to cycloheximide and emetine is higher than that of the plasma cell tumours. In the dose range of 1 - 10(-7) --5 - 10(-5) M cycloheximide and 1 - 10(-6)--1 - 10(-4) M emetine the reticulocyte system is preferentially inhibited; (3) the sensitivities of all plasma cell tumours are equal; (4) the liver system is more sensitive to emetine than to cycloheximide; (5) the site of the different susceptibility to these antibiotics could be located on the ribosomes; (6) however, when the extracts of the plasma cell tumours were prepared in the presence of hemin, their susceptibility rises and is like that of reticulocytes. These results show that hemin promotes in a cell-specific manner the sensitivity to some inhibitors of protein synthesis.", "PMID": 974138}
{"id": "pubmed23n0032_9737", "title": "Inhibition by 5-thio-D-glucopyranose of protein biosynthesis in vitro in spermatids from rat testis.", "content": "The effect of 5-thio-D-glucopyranose (thioglucose) upon protein biosynthesis in vitro was examine in testes from mature rats. Thioglucose in vitro is without demonstrable effect upon incorporation of L-[U-14C]phenylalanine into protein by whole testis but inhibits this incorporation by a purified fraction of immature spermatids (stages 1--8) prepared by centrifugal eluctriation; the inhibition is observed with or without glucose added in vitro and is concentration dependent in the range 1--50 mM. Similar inhibition is observed with three other 14C-labeled amino acids (leucine, lysine and glutamate). Mature spermatids (greater than stage 8) and other heterogenous fractions of testicular cells prepared by the same method also show inhibition by thioglucose of incorporation of phenylalanine into protein so that it is not possible to say that the effect is confined to spermatids although it is most pronounced in these cells. Inhibition of protein synthesis in vitro is also observed when thioglucose was administered in vivo (33 mg/kg body wt./day). This change occurs at the minimal dose observed by other workers to produce arrest of spermatogenesis and hence infertility.", "contents": "Inhibition by 5-thio-D-glucopyranose of protein biosynthesis in vitro in spermatids from rat testis. The effect of 5-thio-D-glucopyranose (thioglucose) upon protein biosynthesis in vitro was examine in testes from mature rats. Thioglucose in vitro is without demonstrable effect upon incorporation of L-[U-14C]phenylalanine into protein by whole testis but inhibits this incorporation by a purified fraction of immature spermatids (stages 1--8) prepared by centrifugal eluctriation; the inhibition is observed with or without glucose added in vitro and is concentration dependent in the range 1--50 mM. Similar inhibition is observed with three other 14C-labeled amino acids (leucine, lysine and glutamate). Mature spermatids (greater than stage 8) and other heterogenous fractions of testicular cells prepared by the same method also show inhibition by thioglucose of incorporation of phenylalanine into protein so that it is not possible to say that the effect is confined to spermatids although it is most pronounced in these cells. Inhibition of protein synthesis in vitro is also observed when thioglucose was administered in vivo (33 mg/kg body wt./day). This change occurs at the minimal dose observed by other workers to produce arrest of spermatogenesis and hence infertility.", "PMID": 974139}
{"id": "pubmed23n0032_9738", "title": "Membrane associated phospholipoproteins of Bacillus lichenformis 749.", "content": "The membrane-bound penicillinase of Bacillus licheniformis 749/C is a phospholipoprotein that differs from the hydrophilic exoenzyme in that its polypeptide chain carries an additional 25 residues (mostly hydrophilic) with phosphatidylserine as the NH2-terminus. To determine if other phospholipoproteins are present in the plasma membrane, the penicillinase-inducible strain 749 was grown without inducer in the presence of [2-(3)H] glycerol. Electrophoretic separation of the membrane proteins (after removal of free lipids) showed an association of 3H-activity with certain of the proteins which could not be broken by lipid solvents and strongly denaturing conditions. Pronase digestion of the membrane proteins (after solvent extraction) released phosphatidylserine, thus indicating the covalent linkage of protein and phospholipid. Treatment of the isolated membranes with trypsin solubilized the protein portion of some of the phospholipoproteins (as with penicillinase), but not the 3H-labelled fragment. Penicillinase should be considered as the first observed example of a group of phosphatidylserine-containing proteins present in the plasma membrane of B. licheniformis 749 and 749/C.", "contents": "Membrane associated phospholipoproteins of Bacillus lichenformis 749. The membrane-bound penicillinase of Bacillus licheniformis 749/C is a phospholipoprotein that differs from the hydrophilic exoenzyme in that its polypeptide chain carries an additional 25 residues (mostly hydrophilic) with phosphatidylserine as the NH2-terminus. To determine if other phospholipoproteins are present in the plasma membrane, the penicillinase-inducible strain 749 was grown without inducer in the presence of [2-(3)H] glycerol. Electrophoretic separation of the membrane proteins (after removal of free lipids) showed an association of 3H-activity with certain of the proteins which could not be broken by lipid solvents and strongly denaturing conditions. Pronase digestion of the membrane proteins (after solvent extraction) released phosphatidylserine, thus indicating the covalent linkage of protein and phospholipid. Treatment of the isolated membranes with trypsin solubilized the protein portion of some of the phospholipoproteins (as with penicillinase), but not the 3H-labelled fragment. Penicillinase should be considered as the first observed example of a group of phosphatidylserine-containing proteins present in the plasma membrane of B. licheniformis 749 and 749/C.", "PMID": 974140}
{"id": "pubmed23n0032_9739", "title": "Factors affecting the compartmentalization of sodium ion within rabbit ileum in vitro.", "content": "(1) Net Na+ loss from rabbit ileum, stripped of its serosal muscle layers, into ice cold choline chloride is consistent with loss from two separate pools (rate constants 0.102 and 0.011 min-1). Since cell K+ is lost with a single rate constant, 0.0062 min-1) and inulin, a good extracellular marker, is lost with a single rate constant 0.082 min-1, it is inferred that the fast rate constant of Na loss characterizes loss from an extracellular pool and the slow constant, loss from an intracellular pool. (2) The [Na+] in the inulin space (extracellular) was calculated to be 180 +/- 13 (S.D.) mequiv. and the [Na+] in the intracellular space 30.4 +/- 4.1 (S.D.) medquiv., this provides evidence that the paracellular spaces are, at least 80 mosmol hypertonic to the external Ringer. (3) There is a saturable galactose-dependent increase in both the intracellular and extracellular [Na+]. Extracellular [Na+] is increased to 236 +/- 22 (S.D.) mequiv. Whilst intracellular [Na+] is increased to 42.6 +/- 8.8 (S.D.) mequiv. when Ringer [galactose] is 10 mM. Galactose-dependent increases in total tissue [Na+] can thus be attributed mainly to the increase in extracellular [Na+]. (4) Extracellular hypertonicity, both in the presence and absence of galactose, is dependent upon the [Na+] of the bathing Ringer. 0.1 mM ouabain abolishes the extracellular hypertonicity. This observed extracellular hypertonicity in normally functioning tissue may provide the driving force for transcellular convective flow of salt, water and sugars.", "contents": "Factors affecting the compartmentalization of sodium ion within rabbit ileum in vitro. (1) Net Na+ loss from rabbit ileum, stripped of its serosal muscle layers, into ice cold choline chloride is consistent with loss from two separate pools (rate constants 0.102 and 0.011 min-1). Since cell K+ is lost with a single rate constant, 0.0062 min-1) and inulin, a good extracellular marker, is lost with a single rate constant 0.082 min-1, it is inferred that the fast rate constant of Na loss characterizes loss from an extracellular pool and the slow constant, loss from an intracellular pool. (2) The [Na+] in the inulin space (extracellular) was calculated to be 180 +/- 13 (S.D.) mequiv. and the [Na+] in the intracellular space 30.4 +/- 4.1 (S.D.) medquiv., this provides evidence that the paracellular spaces are, at least 80 mosmol hypertonic to the external Ringer. (3) There is a saturable galactose-dependent increase in both the intracellular and extracellular [Na+]. Extracellular [Na+] is increased to 236 +/- 22 (S.D.) mequiv. Whilst intracellular [Na+] is increased to 42.6 +/- 8.8 (S.D.) mequiv. when Ringer [galactose] is 10 mM. Galactose-dependent increases in total tissue [Na+] can thus be attributed mainly to the increase in extracellular [Na+]. (4) Extracellular hypertonicity, both in the presence and absence of galactose, is dependent upon the [Na+] of the bathing Ringer. 0.1 mM ouabain abolishes the extracellular hypertonicity. This observed extracellular hypertonicity in normally functioning tissue may provide the driving force for transcellular convective flow of salt, water and sugars.", "PMID": 974141}
{"id": "pubmed23n0032_9740", "title": "Bidirectional sodium ion movements via the paracellular and transcellular routes across short-circuited rabbit ileum.", "content": "1. It has been confirmed that the agent 2,3,6-triaminopyrimidine decreases Na+ conductance in the paracellular pathway of rabbit ileum. 2. Triaminopyrimidine has been used as a means of measuring transcellular bidirectional Na+ flux, and also, of assessing the contribution of the paracellular pathway to transepithlial Na+ flux. 3. Reduction of Ringer [Na+] to 25 mM or incubation with 0.1 mM ouabain reduces paracellular Na+ permeability. This effect may be due to lateral space collapse. Ringer galactose increases serosa to mucosa Na+ flux by a stimulating reflux through the tight junctions. A proportion of net Na+ flux in control tissues is due to asymmetry generated in the paracellular pathway. It is likely that this passive asymmetry results from an osmotic pressure gradient across the tight-junction. 4. Measurement of the tissue isotope specific activity ratio together with bidirectional transcellular Na+ fluxes allows calculation of the four unidirectional fluxes across the mucosal and serosal boundaries. Values obtained for Na+ entry (J12) and exit (J21) across the mucosal boundary are 7.97 alnd 7.13 mumol-cm(-2)-h(-1) respectively. Entry flux (J12) is a saturable function of Ringer [Na+]. The calculated Km is 295 mM and the V is 17.6 mumul-cm(-2)-h(-1). Na+ entry flux is insensitive to ouabain (0.1 mM). Ouabain results in elevation of exit (J21) flux of Na+ across the brush border. D-Galactose causes a saturable increase in Na+ flux (J12) across the mucosal boundary; the Km for this relationship is 1.2 mM and the V 2.17 mumol-cm(-2)-h(-1). The stoichiometry between sugar and Na+ entry is applixmately 1:1. In contrast to the effect of galactose on entry flux, no change in Na+ efflux across the mucosal boundary is observed when Ringer [galactose] is raised. This finding is dissonant with the prediction of the Na+ -gradient hypothesis. The calculated values of exit (J23) and entry (J32) Na+ fluxes across the serosal border are 16.74 and 15.90 mumol-cm(-2)-h(-1). 0.1 mM ouabain markedly reduces both these unidirectional fluxes. This result is consistent with a serosal location of the Na+-pump. Serosal Na+ exit flux J23 increases as a hyperbolic function of Ringer [galactose]. A small galactose-dependent decrease in entry (J32) is also observed. 0.1 mM ouabain abolishes these galactose-dependent changes. 5. The present findings together with those in the previous paper are discussed in relation to the convective-diffusion model for sugar transport.", "contents": "Bidirectional sodium ion movements via the paracellular and transcellular routes across short-circuited rabbit ileum. 1. It has been confirmed that the agent 2,3,6-triaminopyrimidine decreases Na+ conductance in the paracellular pathway of rabbit ileum. 2. Triaminopyrimidine has been used as a means of measuring transcellular bidirectional Na+ flux, and also, of assessing the contribution of the paracellular pathway to transepithlial Na+ flux. 3. Reduction of Ringer [Na+] to 25 mM or incubation with 0.1 mM ouabain reduces paracellular Na+ permeability. This effect may be due to lateral space collapse. Ringer galactose increases serosa to mucosa Na+ flux by a stimulating reflux through the tight junctions. A proportion of net Na+ flux in control tissues is due to asymmetry generated in the paracellular pathway. It is likely that this passive asymmetry results from an osmotic pressure gradient across the tight-junction. 4. Measurement of the tissue isotope specific activity ratio together with bidirectional transcellular Na+ fluxes allows calculation of the four unidirectional fluxes across the mucosal and serosal boundaries. Values obtained for Na+ entry (J12) and exit (J21) across the mucosal boundary are 7.97 alnd 7.13 mumol-cm(-2)-h(-1) respectively. Entry flux (J12) is a saturable function of Ringer [Na+]. The calculated Km is 295 mM and the V is 17.6 mumul-cm(-2)-h(-1). Na+ entry flux is insensitive to ouabain (0.1 mM). Ouabain results in elevation of exit (J21) flux of Na+ across the brush border. D-Galactose causes a saturable increase in Na+ flux (J12) across the mucosal boundary; the Km for this relationship is 1.2 mM and the V 2.17 mumol-cm(-2)-h(-1). The stoichiometry between sugar and Na+ entry is applixmately 1:1. In contrast to the effect of galactose on entry flux, no change in Na+ efflux across the mucosal boundary is observed when Ringer [galactose] is raised. This finding is dissonant with the prediction of the Na+ -gradient hypothesis. The calculated values of exit (J23) and entry (J32) Na+ fluxes across the serosal border are 16.74 and 15.90 mumol-cm(-2)-h(-1). 0.1 mM ouabain markedly reduces both these unidirectional fluxes. This result is consistent with a serosal location of the Na+-pump. Serosal Na+ exit flux J23 increases as a hyperbolic function of Ringer [galactose]. A small galactose-dependent decrease in entry (J32) is also observed. 0.1 mM ouabain abolishes these galactose-dependent changes. 5. The present findings together with those in the previous paper are discussed in relation to the convective-diffusion model for sugar transport.", "PMID": 974142}
{"id": "pubmed23n0032_9741", "title": "Phospholipid membrane stabilization by dimethylsulfoxide and other inducers of Friend leukemic cell differentiation.", "content": "A large number of low molecular weight polar cryoprotective agents have recently been found to induce erythroid differentiation of Friend leukemic cells in vitro. The effect of these agents on membrane fluidity in phospholipid vesicles was studied by determining the solid-to-liquid crystalline phase transition using differential scanning calorimetry. Some of the inducing agents studies were found to raise the normal transition temperature (Tc) by a few degrees. All of these agents were found to produce a separate transition at a much higher temperature. Changes in the head group of the phospholipid, the pH, the presence of divalent cations, and the addition of other membrane-active compounds were found to significantly influence the inducing agent's effects on the Tc of phospholipid membranes. The ability of the different agents to produce a new transition at a high temperature was found to correlate well with their ability to induce Friend leukemic cell differentiation. The possible mechansims of action of the chemical inducers, and the significance of the observed membrane effects on differentiation and malignancy are discussed. It is concluded that inducing agents decrease the fluidity and stabilize phospholipid membranes, and that their effects in cell differentiation might be initiated by a similar change in the properties of cell membranes.", "contents": "Phospholipid membrane stabilization by dimethylsulfoxide and other inducers of Friend leukemic cell differentiation. A large number of low molecular weight polar cryoprotective agents have recently been found to induce erythroid differentiation of Friend leukemic cells in vitro. The effect of these agents on membrane fluidity in phospholipid vesicles was studied by determining the solid-to-liquid crystalline phase transition using differential scanning calorimetry. Some of the inducing agents studies were found to raise the normal transition temperature (Tc) by a few degrees. All of these agents were found to produce a separate transition at a much higher temperature. Changes in the head group of the phospholipid, the pH, the presence of divalent cations, and the addition of other membrane-active compounds were found to significantly influence the inducing agent's effects on the Tc of phospholipid membranes. The ability of the different agents to produce a new transition at a high temperature was found to correlate well with their ability to induce Friend leukemic cell differentiation. The possible mechansims of action of the chemical inducers, and the significance of the observed membrane effects on differentiation and malignancy are discussed. It is concluded that inducing agents decrease the fluidity and stabilize phospholipid membranes, and that their effects in cell differentiation might be initiated by a similar change in the properties of cell membranes.", "PMID": 974143}
{"id": "pubmed23n0032_9742", "title": "Characteristics of changes in the intracellular potential associated with transport of neutral, dibasic and acidic amino acids in Triturus proximal tubule.", "content": "(1)Introduction of L-alanine and L-lysine into the lumen of the proximal tubule of Triturus kidney evoked an immediate and sustained depolarization of the peritubular membrane potential (Epm) and a small increase in the transtubular potential (Ett). L-Aspartate had no effect. (2) The alanine-induced depolarization was absolutely dependent on the presence of Na+, whereas the lysine-induced one was partially dependent on Na+. In the absence of Na+, alanine usually evoked a transient hyperpolarization of the Epm, while lysine evoked a diffusion potential-like PD change. (3) Addition of alanine or lysine to the peritubular fluid did not cause any immediate change in the Epm, but the cells depolarized with a marked time delay. The delayed depolarization could be ascribed to the entrance of amino acids into the lumen through the nephrostromes and the paracellular pathways. (4) Cellular uptake of alanine and lysine was partially dependent on Na+, while that of aspartate was completely dependent on Na+. (5) Characteristics of the observed electrical events were explained in terms of the differences in the charge transfer associated with transport of these amino acids across the luminal membrane.", "contents": "Characteristics of changes in the intracellular potential associated with transport of neutral, dibasic and acidic amino acids in Triturus proximal tubule. (1)Introduction of L-alanine and L-lysine into the lumen of the proximal tubule of Triturus kidney evoked an immediate and sustained depolarization of the peritubular membrane potential (Epm) and a small increase in the transtubular potential (Ett). L-Aspartate had no effect. (2) The alanine-induced depolarization was absolutely dependent on the presence of Na+, whereas the lysine-induced one was partially dependent on Na+. In the absence of Na+, alanine usually evoked a transient hyperpolarization of the Epm, while lysine evoked a diffusion potential-like PD change. (3) Addition of alanine or lysine to the peritubular fluid did not cause any immediate change in the Epm, but the cells depolarized with a marked time delay. The delayed depolarization could be ascribed to the entrance of amino acids into the lumen through the nephrostromes and the paracellular pathways. (4) Cellular uptake of alanine and lysine was partially dependent on Na+, while that of aspartate was completely dependent on Na+. (5) Characteristics of the observed electrical events were explained in terms of the differences in the charge transfer associated with transport of these amino acids across the luminal membrane.", "PMID": 974144}
{"id": "pubmed23n0032_9743", "title": "Antifolate transport in L1210 leukemia cells. Kinetic evidence for the non-identity of carriers for influx and efflux.", "content": "The kinetics of methotrexate transport in L1210 cells are described. Data derived from the measurmenets of initial influx, the complete time-course of uptake, intracellular steady-state level and unidirectional efflux were found to be consistent with a simple empirical equation containing three constants. Properties of the system include the following: (1) saturability of initial influx: (2) approach to steady state during uptake is exponential; (3) the half-time for drug uptake is independent of external concentration and equal to half-time for efflux; and (4) transport is concentrative at low external concentrations, whereas the reverse is true at high external concentrations. These observations are incorporated into a kinetic model which quantitatively accounts for the data on the basis of the hypothesis that influx and efflux take place via different carriers.", "contents": "Antifolate transport in L1210 leukemia cells. Kinetic evidence for the non-identity of carriers for influx and efflux. The kinetics of methotrexate transport in L1210 cells are described. Data derived from the measurmenets of initial influx, the complete time-course of uptake, intracellular steady-state level and unidirectional efflux were found to be consistent with a simple empirical equation containing three constants. Properties of the system include the following: (1) saturability of initial influx: (2) approach to steady state during uptake is exponential; (3) the half-time for drug uptake is independent of external concentration and equal to half-time for efflux; and (4) transport is concentrative at low external concentrations, whereas the reverse is true at high external concentrations. These observations are incorporated into a kinetic model which quantitatively accounts for the data on the basis of the hypothesis that influx and efflux take place via different carriers.", "PMID": 974145}
{"id": "pubmed23n0032_9744", "title": "Membrane transport properties of bovine articular cartilage.", "content": "The thermodynamic parameters which define transport of nonelectrolytes through bovine articular cartilage membranes were evaluated. H3HO, glucose and sucrose were used as permeants. These solutes permeate more readily through the upper layers (near the articular surface) than through the denser deeper layers approaching bone. Cartilage is similar in many respects to a swollen cellulose gel. Viscous-flow contributes importantly to transport within cartilage thus greatly enhancing the movement of nutrients.", "contents": "Membrane transport properties of bovine articular cartilage. The thermodynamic parameters which define transport of nonelectrolytes through bovine articular cartilage membranes were evaluated. H3HO, glucose and sucrose were used as permeants. These solutes permeate more readily through the upper layers (near the articular surface) than through the denser deeper layers approaching bone. Cartilage is similar in many respects to a swollen cellulose gel. Viscous-flow contributes importantly to transport within cartilage thus greatly enhancing the movement of nutrients.", "PMID": 974146}
{"id": "pubmed23n0032_9745", "title": "Active transport of butyrobetaine and carnitine into isolated liver cells.", "content": "1. The liver cells lose the major part of their carnitine during the commonly used isolation procedure by the collagenase-perfusion method. 2. The cells take up carnitine and the carnitine precursor butyrobetaine when these substances are added to the medium. The carnitine content of isolated liver cells can increase to about 15 mM with no apparent harm to the cells. 3. The data indicate the existence of a common carrier in the plasma membrane which mediates the uphill transport of both carnitine and butyrobetaine. The carrier has a high affinity for butyrobetaine (Km=0.5 mM) and a lower one for carnitine (Km=5.6 mM). 4. The intracellular butyrobetaine is hydroxylated to carnitine with a rate of approximately 0.33 mumol-g wet weight-1-h-1 which is sufficient to cover the turn over of carnitine in the whole rat. Carnitine is effectively esterified in the liver cells to acetylcarnitine and long-chain acylcarnitines. 5. Both carnitine and acetylcarnitine are released from the cells. The release of both compounds is probably physiological since it was found that acetylcarnitine constitutes a similar fraction of the total acid soluble carnitine both in the blood and liver of the intact rat.", "contents": "Active transport of butyrobetaine and carnitine into isolated liver cells. 1. The liver cells lose the major part of their carnitine during the commonly used isolation procedure by the collagenase-perfusion method. 2. The cells take up carnitine and the carnitine precursor butyrobetaine when these substances are added to the medium. The carnitine content of isolated liver cells can increase to about 15 mM with no apparent harm to the cells. 3. The data indicate the existence of a common carrier in the plasma membrane which mediates the uphill transport of both carnitine and butyrobetaine. The carrier has a high affinity for butyrobetaine (Km=0.5 mM) and a lower one for carnitine (Km=5.6 mM). 4. The intracellular butyrobetaine is hydroxylated to carnitine with a rate of approximately 0.33 mumol-g wet weight-1-h-1 which is sufficient to cover the turn over of carnitine in the whole rat. Carnitine is effectively esterified in the liver cells to acetylcarnitine and long-chain acylcarnitines. 5. Both carnitine and acetylcarnitine are released from the cells. The release of both compounds is probably physiological since it was found that acetylcarnitine constitutes a similar fraction of the total acid soluble carnitine both in the blood and liver of the intact rat.", "PMID": 974147}
{"id": "pubmed23n0032_9746", "title": "Identification of the milk fat globule membrane proteins. I. Isolation and partial characterization of glycoprotein B.", "content": "The salt soluble proteins from the fat globule membrane of cow's milk were resolved into three fractions by Sephadex column chromatography in sodium dodecyl sulfate. One of the fractions, termed glycoprotein B, was purified by rechromatography to essentially one band on sodium dodecyl sulfate gel electrophoresis. It was found to contain 14% carbohydrate including sialic acid, mannose, galactose, glucose, glucosamine and galactosamine. The amino acid composition of glycoprotein B was determined; it has amino terminal serine and carboxyl terminal leucine. The molecular weight of this glycoprotein as estimated by sodium dodecyl sulfate gel electrophoresis is 49 500.", "contents": "Identification of the milk fat globule membrane proteins. I. Isolation and partial characterization of glycoprotein B. The salt soluble proteins from the fat globule membrane of cow's milk were resolved into three fractions by Sephadex column chromatography in sodium dodecyl sulfate. One of the fractions, termed glycoprotein B, was purified by rechromatography to essentially one band on sodium dodecyl sulfate gel electrophoresis. It was found to contain 14% carbohydrate including sialic acid, mannose, galactose, glucose, glucosamine and galactosamine. The amino acid composition of glycoprotein B was determined; it has amino terminal serine and carboxyl terminal leucine. The molecular weight of this glycoprotein as estimated by sodium dodecyl sulfate gel electrophoresis is 49 500.", "PMID": 974148}
{"id": "pubmed23n0032_9747", "title": "Scorpion neurotoxin. Mode of action on neuromuscular junctions and synaptosomes.", "content": "Electrophysiological analysis of the effects of scorpion toxin I, one of the neurotoxins from the venom of the scorpion Androctonus australis Hector, upon crayfish neuromuscular junctions has shown that the toxin strongly associates with the nerve terminal to stimulate release of neurotransmitters. The biochemical approach has shown that the binding of scorpion toxin I to rat brain synaptosomes is accompanied by a decrease in their capacity to accumulate gamma-aminobutyric acid. The main effect of the toxin is to stimulate neurotransmitter release. The apparent dissociation constant of the toxin-receptor complex is 0.1-0.2 muM at 22 degrees C. The rate of dissociation is so slow that complex formation seems to be quasi-irreversible. The \"quasi-irreversibility\" has also been observed in electrophysiological experiments with the crayfish neuromuscular junction. Tetrodotoxin prevents scorpion toxin I action if it is incubated with synaptosomes or with crayfish neuromuscular junctions before scorpion toxin I application. Tetrodotoxin does not reverse scorpion toxin action if it is added to the preparation after scorpion toxin I. Prevention of scorpion toxin action by tetrodotoxin permits measurements of binding characteristics of this toxin to synaptosomes. The dissociation constant of the tetrodotoxin-receptor complex is 2.2 nM at 22 degrees C. No cooperativity is observed in the binding. Because of its high affinity for synaptosomes (and the \"quasi-irreversibility\" of the binding), scorpion toxin I appears to be a potentially excellent tool for further studies of the molecular mechanism of neurotransmitter secretion.", "contents": "Scorpion neurotoxin. Mode of action on neuromuscular junctions and synaptosomes. Electrophysiological analysis of the effects of scorpion toxin I, one of the neurotoxins from the venom of the scorpion Androctonus australis Hector, upon crayfish neuromuscular junctions has shown that the toxin strongly associates with the nerve terminal to stimulate release of neurotransmitters. The biochemical approach has shown that the binding of scorpion toxin I to rat brain synaptosomes is accompanied by a decrease in their capacity to accumulate gamma-aminobutyric acid. The main effect of the toxin is to stimulate neurotransmitter release. The apparent dissociation constant of the toxin-receptor complex is 0.1-0.2 muM at 22 degrees C. The rate of dissociation is so slow that complex formation seems to be quasi-irreversible. The \"quasi-irreversibility\" has also been observed in electrophysiological experiments with the crayfish neuromuscular junction. Tetrodotoxin prevents scorpion toxin I action if it is incubated with synaptosomes or with crayfish neuromuscular junctions before scorpion toxin I application. Tetrodotoxin does not reverse scorpion toxin action if it is added to the preparation after scorpion toxin I. Prevention of scorpion toxin action by tetrodotoxin permits measurements of binding characteristics of this toxin to synaptosomes. The dissociation constant of the tetrodotoxin-receptor complex is 2.2 nM at 22 degrees C. No cooperativity is observed in the binding. Because of its high affinity for synaptosomes (and the \"quasi-irreversibility\" of the binding), scorpion toxin I appears to be a potentially excellent tool for further studies of the molecular mechanism of neurotransmitter secretion.", "PMID": 974149}
{"id": "pubmed23n0032_9748", "title": "Phosphorylation in isolated chloroplasts coupled to dichlorophenyldimethylurea-insensitive silicomolybdate reduction.", "content": "1. The electron transport in isolated chloroplasts with silicomolybdate as electron acceptor has been reinvestigated. The silicomolybdate reduction has been directly measured as deltaA750 or indirectly as O2 evolution (in the presence or absence of ferricyanide). 2. Silicomolybdate-dependent O2 evolution is inhibited to a similar extent by 3-(3,4-dichlorophenyl) 1, 1-dimethylurea (DCMU) or dibromothymoquinone (DBMIB), indicating the existence of two different sites of silicomolybdate reduction: one before the DCMU block (i.e. at Photosystem II) and one after the DBMIB block (i.e. at Photosystem I). 3. Silicomolybdate-dependent O2 evolution is coupled to ATP synthesis with an ATP/2e- ratio of 1.0 to 1.1. The presence of ferricyanide inhibits this ATP synthesis (ATP/2e- ratio then is about 0.3). 4. Silicomolybdate-dependent O2 evolution is also coupled to ATP-synthesis in the presence of DCMU with an ATP/2e- ratio of 0.6-0.8 characteristic of Site II; in this case the electron transport itself is not affected by uncouplers or energy-transfer inhibitors. 5. The data are interpreted as a further demonstration that the water-splitting reaction is responsible for the conservation of energy at Photosystem II.", "contents": "Phosphorylation in isolated chloroplasts coupled to dichlorophenyldimethylurea-insensitive silicomolybdate reduction. 1. The electron transport in isolated chloroplasts with silicomolybdate as electron acceptor has been reinvestigated. The silicomolybdate reduction has been directly measured as deltaA750 or indirectly as O2 evolution (in the presence or absence of ferricyanide). 2. Silicomolybdate-dependent O2 evolution is inhibited to a similar extent by 3-(3,4-dichlorophenyl) 1, 1-dimethylurea (DCMU) or dibromothymoquinone (DBMIB), indicating the existence of two different sites of silicomolybdate reduction: one before the DCMU block (i.e. at Photosystem II) and one after the DBMIB block (i.e. at Photosystem I). 3. Silicomolybdate-dependent O2 evolution is coupled to ATP synthesis with an ATP/2e- ratio of 1.0 to 1.1. The presence of ferricyanide inhibits this ATP synthesis (ATP/2e- ratio then is about 0.3). 4. Silicomolybdate-dependent O2 evolution is also coupled to ATP-synthesis in the presence of DCMU with an ATP/2e- ratio of 0.6-0.8 characteristic of Site II; in this case the electron transport itself is not affected by uncouplers or energy-transfer inhibitors. 5. The data are interpreted as a further demonstration that the water-splitting reaction is responsible for the conservation of energy at Photosystem II.", "PMID": 974151}
{"id": "pubmed23n0032_9749", "title": "Light-induced absorption changes in photosystem I at low temperatures.", "content": "Light-induced absorption changes associated with the primary photochemical reaction and dark relaxation in Photosystem I were measured at various low temperatures. A possible temperature-dependent long-range electron tunneling process was suggested to account for the unique temperature dependence of the dark decay process. The kinetics of the light-induced absorption changes are in good agreement with the light-induced EPR changes reported earlier (Ke, B., Sugahara, K., Shaw, E.R., Hansen, R. E., Hamilton, W. D. and Beinert, H. (1974) Biochim, Biophys. Acta 368, 401--408) for the same Photosystem I subchloroplast fragment at comparable temperatures. All absorption changes between 400 and 725 nm at 86degreesK have identical kinetics. The light-minus-dark difference spectrum is very similar to that of P-700 at room temperature, with an additional prominent positive change at 690 nm. Possible contributions by P-430 to the blue and red spectral changes were discussed. It was demonstrated that the intensity of the measuring beam has a drastic effect on the light-induced absorption changes of Photosystem I at low temperatures. Various pretreatments of the Photosystem I fragments such as those that photochemically (or chemically) oxidize the primary donor or photoreduce the primary acceptor abolish the subsequent photochemical reaction. Continuous illumination of the Photosystem I fragments before and during freezing has the same effect. In the temperature range of --20 to --60degreesC, an unusual counter absorption change as well as a counter EPR change were observed.", "contents": "Light-induced absorption changes in photosystem I at low temperatures. Light-induced absorption changes associated with the primary photochemical reaction and dark relaxation in Photosystem I were measured at various low temperatures. A possible temperature-dependent long-range electron tunneling process was suggested to account for the unique temperature dependence of the dark decay process. The kinetics of the light-induced absorption changes are in good agreement with the light-induced EPR changes reported earlier (Ke, B., Sugahara, K., Shaw, E.R., Hansen, R. E., Hamilton, W. D. and Beinert, H. (1974) Biochim, Biophys. Acta 368, 401--408) for the same Photosystem I subchloroplast fragment at comparable temperatures. All absorption changes between 400 and 725 nm at 86degreesK have identical kinetics. The light-minus-dark difference spectrum is very similar to that of P-700 at room temperature, with an additional prominent positive change at 690 nm. Possible contributions by P-430 to the blue and red spectral changes were discussed. It was demonstrated that the intensity of the measuring beam has a drastic effect on the light-induced absorption changes of Photosystem I at low temperatures. Various pretreatments of the Photosystem I fragments such as those that photochemically (or chemically) oxidize the primary donor or photoreduce the primary acceptor abolish the subsequent photochemical reaction. Continuous illumination of the Photosystem I fragments before and during freezing has the same effect. In the temperature range of --20 to --60degreesC, an unusual counter absorption change as well as a counter EPR change were observed.", "PMID": 974152}
{"id": "pubmed23n0032_9750", "title": "Positional distribution of fatty acids in rabbit lung phospholipids and triacylglycerols and effect of prolonged hyperoxy.", "content": "Investigations have been carried out for the determination of the effect of high oxygen concentration in inspired gas mixutre on the positional distribution of fatty acids in rabbit lung phospholipids and triacylglycerols. The following results were obtained: 1. In the phosphatidylcholine fraction, the high oxygen concentration caused a quantitative increase of palmitic acid (16 : 0) at the alpha-position, and of myristic (14 : 0), heptadecenic (17 : 1) and arachidonic (20 : 4) acids at the beta-position. 2. In t-e phosphatidylethanolamine fraction, the high oxygen concentration caused an increase of oleic acid (18 : 1) at the alpha-position, and of palmitoleic (16 : 1) and heptadecenic (17 : 1) acids at the beta-position. 3. In the triacylglycerol fraction such changes were not observed. In connection with these effects of oxygen, its possible influence on membrane structures in the lung has been discussed.", "contents": "Positional distribution of fatty acids in rabbit lung phospholipids and triacylglycerols and effect of prolonged hyperoxy. Investigations have been carried out for the determination of the effect of high oxygen concentration in inspired gas mixutre on the positional distribution of fatty acids in rabbit lung phospholipids and triacylglycerols. The following results were obtained: 1. In the phosphatidylcholine fraction, the high oxygen concentration caused a quantitative increase of palmitic acid (16 : 0) at the alpha-position, and of myristic (14 : 0), heptadecenic (17 : 1) and arachidonic (20 : 4) acids at the beta-position. 2. In t-e phosphatidylethanolamine fraction, the high oxygen concentration caused an increase of oleic acid (18 : 1) at the alpha-position, and of palmitoleic (16 : 1) and heptadecenic (17 : 1) acids at the beta-position. 3. In the triacylglycerol fraction such changes were not observed. In connection with these effects of oxygen, its possible influence on membrane structures in the lung has been discussed.", "PMID": 974153}
{"id": "pubmed23n0032_9751", "title": "How valine deprivation and its reversal affect fatty acid metabolism in HeLa cells.", "content": "A protocol based on radio gas chromatography demonstrates that when HeLa cells are deprived of valine for short periods of time (6-7.5 h), their overall fatty acid biosynthetic activity is depressed after a latency of a few hours. The transfer of newly synthesized fatty acyl units to phospholipids is curtailed much faster than their transfer to triacyglycerols. Despite the cut-back in fatty acid biosynthesis, valine deprivation causes a lipid accumulation in the cells. Valine deprivation appears to affect de novo synthesis of fatty acid units from acetate more rapidly than desaturation and elongation. When valine is returned to the valine-deprived culture, overall fatty acid biosynthesis is resumed well within 2 h. Newly synthesized fatty acyl units are transferred to both the phospholipids and the 1,2-diacylglycerols of the cells but not initially to the triacylglycerols.", "contents": "How valine deprivation and its reversal affect fatty acid metabolism in HeLa cells. A protocol based on radio gas chromatography demonstrates that when HeLa cells are deprived of valine for short periods of time (6-7.5 h), their overall fatty acid biosynthetic activity is depressed after a latency of a few hours. The transfer of newly synthesized fatty acyl units to phospholipids is curtailed much faster than their transfer to triacyglycerols. Despite the cut-back in fatty acid biosynthesis, valine deprivation causes a lipid accumulation in the cells. Valine deprivation appears to affect de novo synthesis of fatty acid units from acetate more rapidly than desaturation and elongation. When valine is returned to the valine-deprived culture, overall fatty acid biosynthesis is resumed well within 2 h. Newly synthesized fatty acyl units are transferred to both the phospholipids and the 1,2-diacylglycerols of the cells but not initially to the triacylglycerols.", "PMID": 974154}
{"id": "pubmed23n0032_9752", "title": "Impaired ganglioside synthesis in rat liver after D-galactosamine administration in vivo.", "content": "D-Galactosamine reduces the hepatic content of uridine phosphates, UDP-galactose, and UDPglucose due to an accumulation of UDP-amino sugars; this deficiency can result in severe hepatocellular damage. Alterations of glycosphingolipid synthesis in the early phase of this pathogenic process were studied by measurements of the incorporation of labeled galactose into glycosphingolipids of rat liver. [1-14C]Galactose was injected 2 h after galactosamine administration and the specific radioactivities of the glycosphingolipid precursors, UCPgalactose and UDPglucose, were determined. The specific radioactivity of UDPgalactose, when integrated over the whole period of radioactive synthesis, was four times higher in the galactosamine-treated animals than in the controls; the corresponding ratio of UDPglucose was 0.85. The pattern of the glycosphingolipids isolated from the livers of normal and galactosamine-treated rats resembled that described by Siddiqui and Hakomori (1970, Cancer Res. 30, 2930-2936); GL1, GM3,GM1, GD1, and a small amount of GT could be characterized. The specific radioactivities of glucose and galactose obtained from individual glycosphingolipids were determined in normal and galactosamine-treated livers. The synthesis of the glycosphingolipids was calculated using the respective data of the UDPhexoses. The labeling of glucosylceramide (GL1) was not altered and only a small change of GM3 could be detected; the synthesis of gangliosides GM1 and GD1, however, was inhibited by 95% or more between 4 and 6 h after galactosamine administration.", "contents": "Impaired ganglioside synthesis in rat liver after D-galactosamine administration in vivo. D-Galactosamine reduces the hepatic content of uridine phosphates, UDP-galactose, and UDPglucose due to an accumulation of UDP-amino sugars; this deficiency can result in severe hepatocellular damage. Alterations of glycosphingolipid synthesis in the early phase of this pathogenic process were studied by measurements of the incorporation of labeled galactose into glycosphingolipids of rat liver. [1-14C]Galactose was injected 2 h after galactosamine administration and the specific radioactivities of the glycosphingolipid precursors, UCPgalactose and UDPglucose, were determined. The specific radioactivity of UDPgalactose, when integrated over the whole period of radioactive synthesis, was four times higher in the galactosamine-treated animals than in the controls; the corresponding ratio of UDPglucose was 0.85. The pattern of the glycosphingolipids isolated from the livers of normal and galactosamine-treated rats resembled that described by Siddiqui and Hakomori (1970, Cancer Res. 30, 2930-2936); GL1, GM3,GM1, GD1, and a small amount of GT could be characterized. The specific radioactivities of glucose and galactose obtained from individual glycosphingolipids were determined in normal and galactosamine-treated livers. The synthesis of the glycosphingolipids was calculated using the respective data of the UDPhexoses. The labeling of glucosylceramide (GL1) was not altered and only a small change of GM3 could be detected; the synthesis of gangliosides GM1 and GD1, however, was inhibited by 95% or more between 4 and 6 h after galactosamine administration.", "PMID": 974155}
{"id": "pubmed23n0032_9753", "title": "Feed-back control of cholesterol synthesis in partially hepatectomized rats.", "content": "Partial hepatectomy caused a marked stimulation of cholesterol and fatty acid syntheses without affecting serum total cholesterol, total phospholipid and triacylglycerol concentrations of rats so far examined 48 h after the operation. Serum free cholesterol level, however, was increased by the treatment and the ratio of lysophosphatidylcholine to phosphatidylcholine was concomitantly decreased, suggesting the impairment of serum lecithin: cholesterol acyltransferase activity. The lipid content in the liver, especially triacylglycerol and ester cholesterol, was increased markedly by the operation. Feeding of a high cholesterol diet which elevated serum cholesterol and phospholipid levels to the partially hepatectomized rats, accelerated the accumulation of hepatic triacylglycerol and ester cholesterol by the partial hepatectomy. The weight of the regenerating liver was not influenced by cholesterol feeding, which suggested that cholesterol feeding did not inhibit the regeneration mechanism of the liver. The increase of cholesterol synthesis after partial hepatectomy was inhibited by cholesterol feeding. Therefore, it is conceivable that the negative feed-back control of cholesterol synthesis is induced by cholesterol feeding under the stimulated cell divisions of the liver after partial hepatectomy. It is suggested from the present data that a large amount of the cholesterol which is necessary for cell growth can be taken up from serum, when serum cholesterol concentration is high.", "contents": "Feed-back control of cholesterol synthesis in partially hepatectomized rats. Partial hepatectomy caused a marked stimulation of cholesterol and fatty acid syntheses without affecting serum total cholesterol, total phospholipid and triacylglycerol concentrations of rats so far examined 48 h after the operation. Serum free cholesterol level, however, was increased by the treatment and the ratio of lysophosphatidylcholine to phosphatidylcholine was concomitantly decreased, suggesting the impairment of serum lecithin: cholesterol acyltransferase activity. The lipid content in the liver, especially triacylglycerol and ester cholesterol, was increased markedly by the operation. Feeding of a high cholesterol diet which elevated serum cholesterol and phospholipid levels to the partially hepatectomized rats, accelerated the accumulation of hepatic triacylglycerol and ester cholesterol by the partial hepatectomy. The weight of the regenerating liver was not influenced by cholesterol feeding, which suggested that cholesterol feeding did not inhibit the regeneration mechanism of the liver. The increase of cholesterol synthesis after partial hepatectomy was inhibited by cholesterol feeding. Therefore, it is conceivable that the negative feed-back control of cholesterol synthesis is induced by cholesterol feeding under the stimulated cell divisions of the liver after partial hepatectomy. It is suggested from the present data that a large amount of the cholesterol which is necessary for cell growth can be taken up from serum, when serum cholesterol concentration is high.", "PMID": 974156}
{"id": "pubmed23n0032_9754", "title": "Partial purification and properties of the fatty acid elongation systems in the outer and inner membranes of beef liver mitochondria.", "content": "Purified outer membrane of beef liver mitochondria was found to elongate medium chain fatty acyl-CoA primer by the incorporation of [1-14C]acetyl-CoA. This enzymic activity, extracted by Triton X-100, was purified 8-fold by ammonium sulfate fractionation followed by chromatography on a Sephadex column. Purified inner membrane, when processed through an identical purification procedure, yielded a second enzyme system which incorporated [1-14C]acetyl-CoA into long chain fatty acids in the presence of medium chain fatty acyl-CoA primer. This enzyme preparation was about four times as active as the preparation from the outer membrane, and used NADH as the reductant for the synthesis. The molecular weights of the inner and the outer membrane enzyme systems, estimated by gel filtration as well as sucrose density gradient centrifugation, were approx. 57 000 and 126 000, respectively. The partially purified outer membrane enzyme system required NADH and a medium chain acyl-CoA primer for the incorporation of [1-14C]acetyl-CoA into long chain fatty acids. KNC stimulated the reaction. NADPH could  substitute for NADH only to a limited extent. Malonyl-CoA was ineffective as a substrate in this reaction. The optimum pH of the reaction was 7.2-7.6 in 0.1 M potassium phosphate buffer. Dithiothreitol, beta-mercaptoethanol, N-ethylmaleimide and high concentrations of ATP and acyl-CoA primer inhibited the reaction. The specificity for the acyl-CoA primer in the reaction was very broad. All the primers tested, C8 to C16, incorporated acetyl-CoA significantly. However, maximum incorporation was observed with dodecanoyl-CoA. Decanoyl-CoA was the best primer for the enzyme system isolated from the inner membrane. About 42% of the radioactivity in the fatty acids synthesized by the outer membrane enzyme system, from myristoyl-CoA and [1-C14]acetyl-CoA, was in palmitic acid. Of the remaining activity, 41% was in stearic acid and about 38% in longer-chain acids. Hence, the elongation of the primer fatty acid by one C2 unit appeared to be the predominant process in this synthesis. In the elongation of myristoyl-C0A by the inner membrane enzyme system, palmitic acid which constituted nearly 78% of the fatty acids synthesized, was the primary product.", "contents": "Partial purification and properties of the fatty acid elongation systems in the outer and inner membranes of beef liver mitochondria. Purified outer membrane of beef liver mitochondria was found to elongate medium chain fatty acyl-CoA primer by the incorporation of [1-14C]acetyl-CoA. This enzymic activity, extracted by Triton X-100, was purified 8-fold by ammonium sulfate fractionation followed by chromatography on a Sephadex column. Purified inner membrane, when processed through an identical purification procedure, yielded a second enzyme system which incorporated [1-14C]acetyl-CoA into long chain fatty acids in the presence of medium chain fatty acyl-CoA primer. This enzyme preparation was about four times as active as the preparation from the outer membrane, and used NADH as the reductant for the synthesis. The molecular weights of the inner and the outer membrane enzyme systems, estimated by gel filtration as well as sucrose density gradient centrifugation, were approx. 57 000 and 126 000, respectively. The partially purified outer membrane enzyme system required NADH and a medium chain acyl-CoA primer for the incorporation of [1-14C]acetyl-CoA into long chain fatty acids. KNC stimulated the reaction. NADPH could  substitute for NADH only to a limited extent. Malonyl-CoA was ineffective as a substrate in this reaction. The optimum pH of the reaction was 7.2-7.6 in 0.1 M potassium phosphate buffer. Dithiothreitol, beta-mercaptoethanol, N-ethylmaleimide and high concentrations of ATP and acyl-CoA primer inhibited the reaction. The specificity for the acyl-CoA primer in the reaction was very broad. All the primers tested, C8 to C16, incorporated acetyl-CoA significantly. However, maximum incorporation was observed with dodecanoyl-CoA. Decanoyl-CoA was the best primer for the enzyme system isolated from the inner membrane. About 42% of the radioactivity in the fatty acids synthesized by the outer membrane enzyme system, from myristoyl-CoA and [1-C14]acetyl-CoA, was in palmitic acid. Of the remaining activity, 41% was in stearic acid and about 38% in longer-chain acids. Hence, the elongation of the primer fatty acid by one C2 unit appeared to be the predominant process in this synthesis. In the elongation of myristoyl-C0A by the inner membrane enzyme system, palmitic acid which constituted nearly 78% of the fatty acids synthesized, was the primary product.", "PMID": 974157}
{"id": "pubmed23n0032_9755", "title": "[Cathepsin D from horse spleen. I. Purification and study of certain physicochemical properties].", "content": "Horse spleen cathepsin D (3.4.23.5.) was purified from crude extract by sodium chloride and ethanol precipitation, column chromatography fractionation on DEAE cellulose and CM Sephadex, re-chromatography on DEAE cellulose and gel filtration. The enzyme has been purified about 3.000 folds with a yield of 30 per cent. The purified enzyme seems to be homogeneous on Sephadex G100, one protein band is apparent on disc electrophoresis. Determined by dansylation the N-terminal amino acid is glycine. A molecular weight of 42,500 +/- 3,000 was obtained with Sephadex G100 gel filtration and light scattering measurements. Amino acid analysis and chemical determinations were performed: cathepsin D is a glycoprotein (2 or 3 osamine residues) including 344 amino acids and 4 disulfide bonds. Spectrophotometric data show that E1cm/1 mg/ml = 1.01 at lambda = 280 nm. ORD measurements indicate about 20 per cent of helicoidal content in the molecule.", "contents": "[Cathepsin D from horse spleen. I. Purification and study of certain physicochemical properties]. Horse spleen cathepsin D (3.4.23.5.) was purified from crude extract by sodium chloride and ethanol precipitation, column chromatography fractionation on DEAE cellulose and CM Sephadex, re-chromatography on DEAE cellulose and gel filtration. The enzyme has been purified about 3.000 folds with a yield of 30 per cent. The purified enzyme seems to be homogeneous on Sephadex G100, one protein band is apparent on disc electrophoresis. Determined by dansylation the N-terminal amino acid is glycine. A molecular weight of 42,500 +/- 3,000 was obtained with Sephadex G100 gel filtration and light scattering measurements. Amino acid analysis and chemical determinations were performed: cathepsin D is a glycoprotein (2 or 3 osamine residues) including 344 amino acids and 4 disulfide bonds. Spectrophotometric data show that E1cm/1 mg/ml = 1.01 at lambda = 280 nm. ORD measurements indicate about 20 per cent of helicoidal content in the molecule.", "PMID": 974159}
{"id": "pubmed23n0032_9756", "title": "Ultrastructure of the atypic muscles associated with terminalial inversion in male Aedes aegypti (L).", "content": "Two sets of opposed, crossed muscles are present in the rotating region of the abdomen in male A. aegypti. These muscles undergo changes during rotation of the genitalia that suggest they function as the driving force for rotation. During this rotation, one muscle of each set contracts and the opposed one becomes elongated. The contracting muscles are atypic physiologically. They contract from 300 mum to about 69 mum, and this requires a period of 18 to 24 hours. They shorten only once and those muscle fibers still present after the completion of rotation remain in a contracted condition at least for two weeks. The elongated muscles never shorten; they become stretched to approximately 2.5 times their original length and disappear soon after rotation is completed.", "contents": "Ultrastructure of the atypic muscles associated with terminalial inversion in male Aedes aegypti (L). Two sets of opposed, crossed muscles are present in the rotating region of the abdomen in male A. aegypti. These muscles undergo changes during rotation of the genitalia that suggest they function as the driving force for rotation. During this rotation, one muscle of each set contracts and the opposed one becomes elongated. The contracting muscles are atypic physiologically. They contract from 300 mum to about 69 mum, and this requires a period of 18 to 24 hours. They shorten only once and those muscle fibers still present after the completion of rotation remain in a contracted condition at least for two weeks. The elongated muscles never shorten; they become stretched to approximately 2.5 times their original length and disappear soon after rotation is completed.", "PMID": 974162}
{"id": "pubmed23n0032_9757", "title": "Permeability of trout erythrocytes to nonelectrolytes.", "content": "Using a densimeter technique, a kinetic analysis was made, employing both entrance and exit studies, of the permeability of erythrocytes of brook trout (Salvelinus fontinalis), rainbow trout (Salmo gairdneri), German brown trout (Salmo trutta) and cutthroat trout (Salmo clarki) to glycerol, ethylene glycol, thiourea and urea. All of the data indicate that these four nonelectrolytes cross the membrane of the erythrocytes of these four species of fishes by simple diffusion only.", "contents": "Permeability of trout erythrocytes to nonelectrolytes. Using a densimeter technique, a kinetic analysis was made, employing both entrance and exit studies, of the permeability of erythrocytes of brook trout (Salvelinus fontinalis), rainbow trout (Salmo gairdneri), German brown trout (Salmo trutta) and cutthroat trout (Salmo clarki) to glycerol, ethylene glycol, thiourea and urea. All of the data indicate that these four nonelectrolytes cross the membrane of the erythrocytes of these four species of fishes by simple diffusion only.", "PMID": 974163}
{"id": "pubmed23n0032_9758", "title": "Histochemical changes in gonadal nutrient reserves correlated with nutrition in the sea stars, Pisaster ochraceus and Patiria miniata.", "content": "1. Pisaster ochraceus has an annual reproductive cycle in which the gonads increase in size rapidly from October or November and reach a maximum in March to May, after which spawning occurs. In Patiria miniata the gonadal cycle is less pronounced and less regular. In the course of this study, animals spawned in July 1965, May 1966, and May 1967. 2. Histochemical techniques indicate that in both species glycogen or a glycogen-like carbohydrate occurs in the germinal epithelium and the follicle cells of the female, and in the spermatogonia and primary spermatocytes. A storage carbohydrate which is not removed by diastase or amylase is abundant in oocytes in the form of yolk granules 0.5 to 1.5 mu in diameter. 3. Neutral lipid droplets and phospholipid granules are abundant in all oocytes but the smallest. In the testes, lipid droplets are seen only after prolonged starvation. 4. In both species prolonged starvation results in failure of the gonads to achieve their normal size increase. Such gametes as are seen in starving speciments appear histochemically normal in some instances; in other cases they seem to be undergoing breakdown. 5. The histochemical results concerning nutrient reserves of the gonads are generally in agreement with the biochemical findings of earlier workers.", "contents": "Histochemical changes in gonadal nutrient reserves correlated with nutrition in the sea stars, Pisaster ochraceus and Patiria miniata. 1. Pisaster ochraceus has an annual reproductive cycle in which the gonads increase in size rapidly from October or November and reach a maximum in March to May, after which spawning occurs. In Patiria miniata the gonadal cycle is less pronounced and less regular. In the course of this study, animals spawned in July 1965, May 1966, and May 1967. 2. Histochemical techniques indicate that in both species glycogen or a glycogen-like carbohydrate occurs in the germinal epithelium and the follicle cells of the female, and in the spermatogonia and primary spermatocytes. A storage carbohydrate which is not removed by diastase or amylase is abundant in oocytes in the form of yolk granules 0.5 to 1.5 mu in diameter. 3. Neutral lipid droplets and phospholipid granules are abundant in all oocytes but the smallest. In the testes, lipid droplets are seen only after prolonged starvation. 4. In both species prolonged starvation results in failure of the gonads to achieve their normal size increase. Such gametes as are seen in starving speciments appear histochemically normal in some instances; in other cases they seem to be undergoing breakdown. 5. The histochemical results concerning nutrient reserves of the gonads are generally in agreement with the biochemical findings of earlier workers.", "PMID": 974164}
{"id": "pubmed23n0032_9759", "title": "[Conformational heterogeneity of tRNA, detected in the reaction of guanine bases with ketoxal].", "content": "The study of kinetic characteristics of the reaction of tRNA guanine bases with kethoxal has shown that temperature, ionic strength and Mg2+ ions, i.e. factors directly affecting the spatial structure of tRNA, influence also on its internal modification. The modification degree under stabilized spatial tRNA structure depends also on the concentration of kethoxal and is expressed in fractional values of the number of modified guanosine residues per tRNA molecule, which indicates the heterogeneity of tRNA for the modification degree. Chromatography of tRNA1 Val preparation on BD cellulose after the exhaustive modification with kethoxal under conditions of stabilized spatial structure has revealed a fraction of molecules completely resistant to the modificator, and a fraction containing differently modified tRNA molecules. tRNA heterogeneity after the reaction with kethoxal (the presence of resistant and reactive forms) indicates conformational heterogeneity of tRNA, expressed in the simultaneous presence of at least two conformer families.", "contents": "[Conformational heterogeneity of tRNA, detected in the reaction of guanine bases with ketoxal]. The study of kinetic characteristics of the reaction of tRNA guanine bases with kethoxal has shown that temperature, ionic strength and Mg2+ ions, i.e. factors directly affecting the spatial structure of tRNA, influence also on its internal modification. The modification degree under stabilized spatial tRNA structure depends also on the concentration of kethoxal and is expressed in fractional values of the number of modified guanosine residues per tRNA molecule, which indicates the heterogeneity of tRNA for the modification degree. Chromatography of tRNA1 Val preparation on BD cellulose after the exhaustive modification with kethoxal under conditions of stabilized spatial structure has revealed a fraction of molecules completely resistant to the modificator, and a fraction containing differently modified tRNA molecules. tRNA heterogeneity after the reaction with kethoxal (the presence of resistant and reactive forms) indicates conformational heterogeneity of tRNA, expressed in the simultaneous presence of at least two conformer families.", "PMID": 974169}
{"id": "pubmed23n0032_9760", "title": "[Structure of electrochemically reduced adenine nucleotides. Their interaction with inorganic phosphate].", "content": "The stable reduced form of adenosine phosphates has probably cyclic or dimeric structure of the type: adenine \"head\"--phosphate \"end\". Inorganic phosphate produce a stabilyzing effect on reduced adenosine due to a strong ionic interaction with the amino group. In the case of reduced ADP, inorganic phosphate can incorporate into adenine nucleotide, which oxidation results in the formation of small amounts of ATP. A scheme of possible mechanism of this process is given.", "contents": "[Structure of electrochemically reduced adenine nucleotides. Their interaction with inorganic phosphate]. The stable reduced form of adenosine phosphates has probably cyclic or dimeric structure of the type: adenine \"head\"--phosphate \"end\". Inorganic phosphate produce a stabilyzing effect on reduced adenosine due to a strong ionic interaction with the amino group. In the case of reduced ADP, inorganic phosphate can incorporate into adenine nucleotide, which oxidation results in the formation of small amounts of ATP. A scheme of possible mechanism of this process is given.", "PMID": 974170}
{"id": "pubmed23n0032_9761", "title": "[Heterogeneity of crystalline antithyroid phytoprecipitin].", "content": "The crystalline preparation of antithyroid phytoprecipitin has been resolved by chromatography on Whatman CM-32 cellulose on a preparative scale into two components, designed respectively A and B. Each component was further resolved into consisting polypeptide chains alpha (mol. weight 7.000) and beta (mol. weight 17.000) by gel filtration on sephadex G-50 in 1 M acetic acid 6 M urea. Homologous chains were comparatively studied by electrophoresis in acrylamide gel, amino acid analysis and peptide mapping technique. Electrophoresis in acrylamide gel with 6 M urea according to Takayama [8] revealed the identical mobility of beta chains in both components A and B, while alpha chains differed. alphaA chain was more basic, than alphaB, i.e. it had greater positive sharge. The amino acid analysis and peptide mapping showed that alphaA chain had one residue of lysine more than alphaB chain. The comparison of beta chains by peptid mapping confirmed their complete identity.", "contents": "[Heterogeneity of crystalline antithyroid phytoprecipitin]. The crystalline preparation of antithyroid phytoprecipitin has been resolved by chromatography on Whatman CM-32 cellulose on a preparative scale into two components, designed respectively A and B. Each component was further resolved into consisting polypeptide chains alpha (mol. weight 7.000) and beta (mol. weight 17.000) by gel filtration on sephadex G-50 in 1 M acetic acid 6 M urea. Homologous chains were comparatively studied by electrophoresis in acrylamide gel, amino acid analysis and peptide mapping technique. Electrophoresis in acrylamide gel with 6 M urea according to Takayama [8] revealed the identical mobility of beta chains in both components A and B, while alpha chains differed. alphaA chain was more basic, than alphaB, i.e. it had greater positive sharge. The amino acid analysis and peptide mapping showed that alphaA chain had one residue of lysine more than alphaB chain. The comparison of beta chains by peptid mapping confirmed their complete identity.", "PMID": 974172}
{"id": "pubmed23n0032_9762", "title": "[Localization of pyruvic acid in the histidine decarboxylase of Micrococcus sp. n].", "content": "A fragment containing the pyruvic acid residue is isolated from hymotryptic hydrolyzate of alpha-polypeptide chain of carboxymethylated histidine decarboxylase (HD) 14C-phenylhydrasone. The pyruvate residue is estimated to be bind with alpha-amino group of N-terminal phenylalanine in alpha-polypeptide chain. N-terminal alanine is found in HD alpha-polypeptide chain after its reductive amination. It makes possible to determine the estimation of N-terminal amino acid sequence in HD alpha-chain.", "contents": "[Localization of pyruvic acid in the histidine decarboxylase of Micrococcus sp. n]. A fragment containing the pyruvic acid residue is isolated from hymotryptic hydrolyzate of alpha-polypeptide chain of carboxymethylated histidine decarboxylase (HD) 14C-phenylhydrasone. The pyruvate residue is estimated to be bind with alpha-amino group of N-terminal phenylalanine in alpha-polypeptide chain. N-terminal alanine is found in HD alpha-polypeptide chain after its reductive amination. It makes possible to determine the estimation of N-terminal amino acid sequence in HD alpha-chain.", "PMID": 974173}
{"id": "pubmed23n0032_9763", "title": "[Comparative study of myosin and DTNB-treated myosin with regard to ATP activity and fluorescence].", "content": "When studying enzymic and fluorescence properties of myosin and DTNB-treated myosin in the presence of K+, Na+, Li+, NH4+, Ca2+ and Mg2+ cations the following results were obtained. By the intrinsic protein fluorescence techniques no essential structural changes of myosin molecule at the dissociation of the DTNB light chain and activation myosin ATPase in the presence of different cations were found. The decrease of K+-EDTA-, the increase of Mg2+-activated and the stability of Ca2+-activated myosin ATPase may be the result of the modification of SH1 or SH2 sulfhydryl groups when treating the DTNB myosin in our conditions. The different level of decrease of the K+- and NH4+-activated myosin. ATPase may be explained by the fact, that myosin sulfhydryl groups have different effects on the activation of its ATPase by these cations.", "contents": "[Comparative study of myosin and DTNB-treated myosin with regard to ATP activity and fluorescence]. When studying enzymic and fluorescence properties of myosin and DTNB-treated myosin in the presence of K+, Na+, Li+, NH4+, Ca2+ and Mg2+ cations the following results were obtained. By the intrinsic protein fluorescence techniques no essential structural changes of myosin molecule at the dissociation of the DTNB light chain and activation myosin ATPase in the presence of different cations were found. The decrease of K+-EDTA-, the increase of Mg2+-activated and the stability of Ca2+-activated myosin ATPase may be the result of the modification of SH1 or SH2 sulfhydryl groups when treating the DTNB myosin in our conditions. The different level of decrease of the K+- and NH4+-activated myosin. ATPase may be explained by the fact, that myosin sulfhydryl groups have different effects on the activation of its ATPase by these cations.", "PMID": 974174}
{"id": "pubmed23n0032_9764", "title": "[Separation of leghemoglobin from the nodules of lupine, Lupinus luteus L., into components].", "content": "The crude leghaemoglobin suspension from root nodules of yellow lupine (Lupinus luteus L.) was separated into five fractions: I, IIa and IIb, IIIa and IIIb using the chromatography on DEAE-cellulose (numbered following the elution order). The visible absorption spectra show that fractions I, IIa and IIIa are met-leghaemoglobins while IIb and IIIb are oxy-forms. IIb and IIIb were converted to IIa and IIIa respectively when oxidized with potassium ferricyanide. The determination of two first N-terminal amino acids confirms the identity of pairs IIa-IIb and IIIa-IIIb. Three components of leghaemoglobin were obtained having the following N-terminal amino acids: Gly-Val- (the first), Gly-Ala- (the second), Ala-Val- (the third). The molecular weights obtained by Archibald's method are 17 900, 17 600, 18 800 respectively. The second leghaemoglobin species which contains more than 50% of the starting protein mixture was used to grow crystals for X-ray study of the tertiary structure of this protein.", "contents": "[Separation of leghemoglobin from the nodules of lupine, Lupinus luteus L., into components]. The crude leghaemoglobin suspension from root nodules of yellow lupine (Lupinus luteus L.) was separated into five fractions: I, IIa and IIb, IIIa and IIIb using the chromatography on DEAE-cellulose (numbered following the elution order). The visible absorption spectra show that fractions I, IIa and IIIa are met-leghaemoglobins while IIb and IIIb are oxy-forms. IIb and IIIb were converted to IIa and IIIa respectively when oxidized with potassium ferricyanide. The determination of two first N-terminal amino acids confirms the identity of pairs IIa-IIb and IIIa-IIIb. Three components of leghaemoglobin were obtained having the following N-terminal amino acids: Gly-Val- (the first), Gly-Ala- (the second), Ala-Val- (the third). The molecular weights obtained by Archibald's method are 17 900, 17 600, 18 800 respectively. The second leghaemoglobin species which contains more than 50% of the starting protein mixture was used to grow crystals for X-ray study of the tertiary structure of this protein.", "PMID": 974175}
{"id": "pubmed23n0032_9765", "title": "[Pathways of corticosteroid biosynthesis in human adrenals (Itsenko-Cushing disease)].", "content": "Pregnenolone and its hydroxyl derivatives are of dominant importance in cortisol and corticosterone synthesis in human adrenals (hyperplasic glands and adenomes) removed in Itsenko-Kushing disease. Progesterone is produced in minimal amounts and is nearly not used for steroidogenesis. Participation of 17,21-dihydroxypregnenolone in cortisol synthesis is demonstrated. 17-hydroxyprogesterone is found not to take an important part as cortisol precursor. 21-hydroxypregnenolone is an essential intermediate in corticosterone synthesis. 11-hydroxyprogesterone does not participate in corticosteroids formation, since the rate of progesterone 11-hydroxylation in human adrenal mitochondria is minimal.", "contents": "[Pathways of corticosteroid biosynthesis in human adrenals (Itsenko-Cushing disease)]. Pregnenolone and its hydroxyl derivatives are of dominant importance in cortisol and corticosterone synthesis in human adrenals (hyperplasic glands and adenomes) removed in Itsenko-Kushing disease. Progesterone is produced in minimal amounts and is nearly not used for steroidogenesis. Participation of 17,21-dihydroxypregnenolone in cortisol synthesis is demonstrated. 17-hydroxyprogesterone is found not to take an important part as cortisol precursor. 21-hydroxypregnenolone is an essential intermediate in corticosterone synthesis. 11-hydroxyprogesterone does not participate in corticosteroids formation, since the rate of progesterone 11-hydroxylation in human adrenal mitochondria is minimal.", "PMID": 974176}
{"id": "pubmed23n0032_9766", "title": "[Isolation of cytochrome b556 from the membranes of Micrococcus lysodeikticus bacteria].", "content": "Proteins and cytochrome b556 were solubilized from Micrococcus lysodeikticus membranes using Triton X-100 treatment. Passing of this preparation through DEAE cellulose column in the presence of Triton X-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up to the content of 2.3 nmol per mg of protein. The prostetic group of cytochrome b556 is determined to be protoheme for the spectrum of alkaline pyridinehemochrome.", "contents": "[Isolation of cytochrome b556 from the membranes of Micrococcus lysodeikticus bacteria]. Proteins and cytochrome b556 were solubilized from Micrococcus lysodeikticus membranes using Triton X-100 treatment. Passing of this preparation through DEAE cellulose column in the presence of Triton X-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up to the content of 2.3 nmol per mg of protein. The prostetic group of cytochrome b556 is determined to be protoheme for the spectrum of alkaline pyridinehemochrome.", "PMID": 974177}
{"id": "pubmed23n0032_9767", "title": "[Effect of univalent cations on the glutamate dehydrogenase of chlorella].", "content": "Effect of univalent cations (Li+, K+, Na+ and Cs+) on the activity and some kinetic properties of the constitutive and the inducible glutamate dehydrogenases (GDH) of Chlorella pyrenoidosa Pringsheim 82T has been studied. All the cations used activate the inducible GDH and produced no such effect on the constitutive GDH. From the analysis of the kinetic behaviour in the presence of K+ the conclusion was made that K+ promotes and stabilyzes a catalitically advantagenous conformation of the inducible GDH. This phenomenon appears to have a physiological meaning, because of a higher K+ concentration in Chlorella cells (about 0.1 M) and its important role in metabolism.", "contents": "[Effect of univalent cations on the glutamate dehydrogenase of chlorella]. Effect of univalent cations (Li+, K+, Na+ and Cs+) on the activity and some kinetic properties of the constitutive and the inducible glutamate dehydrogenases (GDH) of Chlorella pyrenoidosa Pringsheim 82T has been studied. All the cations used activate the inducible GDH and produced no such effect on the constitutive GDH. From the analysis of the kinetic behaviour in the presence of K+ the conclusion was made that K+ promotes and stabilyzes a catalitically advantagenous conformation of the inducible GDH. This phenomenon appears to have a physiological meaning, because of a higher K+ concentration in Chlorella cells (about 0.1 M) and its important role in metabolism.", "PMID": 974178}
{"id": "pubmed23n0032_9768", "title": "[Antagonism in the action of hydrocortisone and insulin in vivo on enzymes of pyruvate and malate metabolism in adipose tissue].", "content": "Alloxan diabetes and injections of hydrocortisone into intact animals for 5-7 days resulted in a sharp decrease of NADP-dependent malate dehydrogenase in rat epididymal adipose tissue. Combined injection of insulin and hydrocortisone did not produce the decrease of the enzyme activity. Insulin injections into alloxan diabetic rats recovered the activity of NADP-malate dehydrogenase up to the control. Pyruvate kinase activity was decreased under diabetes, and insulin injections produced further decrease of the enzyme activity in diabetic rats. Activities of lactate dehydrogenase and NAD-dependent malate dehydrogenase were less decreased under diabetes. Comparison of lactate dehydrogenase isoenzymes spectrum in adipose tissue of normal and diabetic rats revealed a considerable increase of LDH-1 and a decrease of LDH-4 under diabetes. Insulin injections greatly normalized LDH isoenzyme spectrum.", "contents": "[Antagonism in the action of hydrocortisone and insulin in vivo on enzymes of pyruvate and malate metabolism in adipose tissue]. Alloxan diabetes and injections of hydrocortisone into intact animals for 5-7 days resulted in a sharp decrease of NADP-dependent malate dehydrogenase in rat epididymal adipose tissue. Combined injection of insulin and hydrocortisone did not produce the decrease of the enzyme activity. Insulin injections into alloxan diabetic rats recovered the activity of NADP-malate dehydrogenase up to the control. Pyruvate kinase activity was decreased under diabetes, and insulin injections produced further decrease of the enzyme activity in diabetic rats. Activities of lactate dehydrogenase and NAD-dependent malate dehydrogenase were less decreased under diabetes. Comparison of lactate dehydrogenase isoenzymes spectrum in adipose tissue of normal and diabetic rats revealed a considerable increase of LDH-1 and a decrease of LDH-4 under diabetes. Insulin injections greatly normalized LDH isoenzyme spectrum.", "PMID": 974179}
{"id": "pubmed23n0032_9769", "title": "[Frequency distribution of pyrimidine isopliths in the DNA of actinophage F448 and Actinomyces coelicolor 66].", "content": "Base composition distribution of pyrimidine isopliths with general formula PyrnPn+1 in DNA of Actinomyces coelicolor 66 Kras. and in DNK of its actinophage F448 were investigater. These DNAs differ both pyrimidine isoplith distribution and in composition. The quality of shorter pyrimidine tracts in Actinomyces coelicolor DNA is higher compared with that in actinophage F448 DNA. The possibility of exogenic origin of actinophage F448 is discussed.", "contents": "[Frequency distribution of pyrimidine isopliths in the DNA of actinophage F448 and Actinomyces coelicolor 66]. Base composition distribution of pyrimidine isopliths with general formula PyrnPn+1 in DNA of Actinomyces coelicolor 66 Kras. and in DNK of its actinophage F448 were investigater. These DNAs differ both pyrimidine isoplith distribution and in composition. The quality of shorter pyrimidine tracts in Actinomyces coelicolor DNA is higher compared with that in actinophage F448 DNA. The possibility of exogenic origin of actinophage F448 is discussed.", "PMID": 974180}
{"id": "pubmed23n0032_9770", "title": "[Extraction and properties of microcapsulated alpha-chymotrypsin].", "content": "A method of microencapsulating of the proteolytic enzyme alpha-chymotrypsin into semi-permeable nylon membranes is worked out. The membrane is a polimer of 1,6-hexamethylenediamine and sebacoyl chloride. alpha-Chymotrypsin is enclosed into the capsule together with polyethyleneimine, capable of joining the walls of microcapsules and making the membrane more stable. The optimal concentrations of polyenthyleneimine and alpha-chymotrypsin are 5% and 1% correspondingly. The highest yield of microencapsulated enzyme was obtained for completely acetylated delta-chymotrypsin. The kinetic properties of microencapsulated alpha-chymotrypsin change very slightly as compared to those of the native one.", "contents": "[Extraction and properties of microcapsulated alpha-chymotrypsin]. A method of microencapsulating of the proteolytic enzyme alpha-chymotrypsin into semi-permeable nylon membranes is worked out. The membrane is a polimer of 1,6-hexamethylenediamine and sebacoyl chloride. alpha-Chymotrypsin is enclosed into the capsule together with polyethyleneimine, capable of joining the walls of microcapsules and making the membrane more stable. The optimal concentrations of polyenthyleneimine and alpha-chymotrypsin are 5% and 1% correspondingly. The highest yield of microencapsulated enzyme was obtained for completely acetylated delta-chymotrypsin. The kinetic properties of microencapsulated alpha-chymotrypsin change very slightly as compared to those of the native one.", "PMID": 974181}
{"id": "pubmed23n0032_9771", "title": "[Isoelectric focusing of plasma membrane proteins from Strongylocentrotus intermedius sea urchin embryo cells].", "content": "14C-amino acids were introduced into the developing sea urchin embryos S. intermedius in 7,5 hours after the fertilization (middle blastula stage). In 30 min the embryos development were terminated, the plasma membrane was isolated and 14C-labelled proteins were recovered from the membrane with 8 M urea and separated by isoelectrofocusing in pH gradient 3,5-10 in presence of 6 M urea. All 14C-label was introduced into two proteins with pI 4,85-4,90 and 5,20-5,25 and the proteins were effectively separated. The minimal molecular weight of the proteins estimated by SDS-acrylamide gel-electrophoresis was 10 000 and 15 000 daltons, respectively.", "contents": "[Isoelectric focusing of plasma membrane proteins from Strongylocentrotus intermedius sea urchin embryo cells]. 14C-amino acids were introduced into the developing sea urchin embryos S. intermedius in 7,5 hours after the fertilization (middle blastula stage). In 30 min the embryos development were terminated, the plasma membrane was isolated and 14C-labelled proteins were recovered from the membrane with 8 M urea and separated by isoelectrofocusing in pH gradient 3,5-10 in presence of 6 M urea. All 14C-label was introduced into two proteins with pI 4,85-4,90 and 5,20-5,25 and the proteins were effectively separated. The minimal molecular weight of the proteins estimated by SDS-acrylamide gel-electrophoresis was 10 000 and 15 000 daltons, respectively.", "PMID": 974182}
{"id": "pubmed23n0032_9772", "title": "Amino acid uptake by fetal kidney slices.", "content": "Kidney slices of 7-17-week-old human fetuses were incubated for 90 min or less in Krebs-Ringer solution containing aminoisobutyric acid (AIB). Cell to medium ratios of AIB decrease with increasing fetal age. Uptake rate is slower than for mature human tissue. Both water content and extracellular space is high in embryonic tissue as compared to adult.", "contents": "Amino acid uptake by fetal kidney slices. Kidney slices of 7-17-week-old human fetuses were incubated for 90 min or less in Krebs-Ringer solution containing aminoisobutyric acid (AIB). Cell to medium ratios of AIB decrease with increasing fetal age. Uptake rate is slower than for mature human tissue. Both water content and extracellular space is high in embryonic tissue as compared to adult.", "PMID": 974183}
{"id": "pubmed23n0032_9773", "title": "A survey of X chromatin in the newborn.", "content": "Buccal smears were analyzed for X chromatin in 1,000 newborn infants. The mean percentage of X chromatin in the 506 female infants was 39.5 (range 19-61) and in the 494 males 0.13 (range 0-3.0). Evaluation of the data comparing the percentage of X chromatin with caucasian and noncaucasian infants, the weight of the newborn, and the gestation time, indicated no significant differences within the male or female populations. A longitudinal study on 25 female infants for the first 4 days postpartum indicated a significant increase in the percentage of X chromatin in females.", "contents": "A survey of X chromatin in the newborn. Buccal smears were analyzed for X chromatin in 1,000 newborn infants. The mean percentage of X chromatin in the 506 female infants was 39.5 (range 19-61) and in the 494 males 0.13 (range 0-3.0). Evaluation of the data comparing the percentage of X chromatin with caucasian and noncaucasian infants, the weight of the newborn, and the gestation time, indicated no significant differences within the male or female populations. A longitudinal study on 25 female infants for the first 4 days postpartum indicated a significant increase in the percentage of X chromatin in females.", "PMID": 974184}
{"id": "pubmed23n0032_9774", "title": "Blood lipids during normal and early weaning in rats.", "content": "In young rats reared with their mothers, a gradual increase of serum cholesterol, triglycerides, phospholipids and total lipids was found between the 7th and the 18th to 22nd postnatal day. Then the concentrations of lipids gradually decrease. Early weaning at the 18th postnatal day causes a precocious decrease of the concentration of all studied lipids in blood so that their values on days 22 and 26 are significantly lower than in animals weaned on the 30th postnatal day. The body weight of early weaned rats decreases only slightly and returns to normal by the 30th day. The changes in serum lipids after early weaning are caused by differences in the amount of cholesterol and other lipids in breast milk, in comparison with those in the standard laboratory diet. Perhaps they may participate in disturbing the homeostatic equilibrium of cholesterol in early weaned rats.", "contents": "Blood lipids during normal and early weaning in rats. In young rats reared with their mothers, a gradual increase of serum cholesterol, triglycerides, phospholipids and total lipids was found between the 7th and the 18th to 22nd postnatal day. Then the concentrations of lipids gradually decrease. Early weaning at the 18th postnatal day causes a precocious decrease of the concentration of all studied lipids in blood so that their values on days 22 and 26 are significantly lower than in animals weaned on the 30th postnatal day. The body weight of early weaned rats decreases only slightly and returns to normal by the 30th day. The changes in serum lipids after early weaning are caused by differences in the amount of cholesterol and other lipids in breast milk, in comparison with those in the standard laboratory diet. Perhaps they may participate in disturbing the homeostatic equilibrium of cholesterol in early weaned rats.", "PMID": 974185}
{"id": "pubmed23n0032_9775", "title": "Lack of correlation between clinical data and growth hormone concentrations in cord blood.", "content": "Growth hormone concentrations were measured in 182 umbilical cord blood samples taken at the time of birth. The mean value in 165 full-term, normal weight infants was 31.5 +/- 25.9 ng/ml SD. 14 prematures had significantly higher levels (50.0 +/- 38.4 p less than 0.01). There were no statistically significant correlations to any of the following parameters: complication of pregnancy by toxaemia, duration of labour, presence of umbilical cord contortion, perinatal distress, Apgar index, mode of delivery, body weight, body length, ratio of weight to length, and blood glucose. There was a wide scatter of individual values in this study as well as in previous investigations. We think it unlikely that those variations are purely accidental. Hence, yet undefined factors must play a major role in the regulation of GH secretion in the perinatal period.", "contents": "Lack of correlation between clinical data and growth hormone concentrations in cord blood. Growth hormone concentrations were measured in 182 umbilical cord blood samples taken at the time of birth. The mean value in 165 full-term, normal weight infants was 31.5 +/- 25.9 ng/ml SD. 14 prematures had significantly higher levels (50.0 +/- 38.4 p less than 0.01). There were no statistically significant correlations to any of the following parameters: complication of pregnancy by toxaemia, duration of labour, presence of umbilical cord contortion, perinatal distress, Apgar index, mode of delivery, body weight, body length, ratio of weight to length, and blood glucose. There was a wide scatter of individual values in this study as well as in previous investigations. We think it unlikely that those variations are purely accidental. Hence, yet undefined factors must play a major role in the regulation of GH secretion in the perinatal period.", "PMID": 974186}
{"id": "pubmed23n0032_9776", "title": "Adenylate cyclase activity in human tissues.", "content": "Adenylate cyclase activity in liver, lung, pancreas, and heart ventricle, from full-term and premature newborns has been assayed. Of all tissues studied, heart had the highest activity. Fluoride 10 mM stimulates adenylate cyclase in all tissues. Glucagon evoked a small activation of liver adenylate cyclase.", "contents": "Adenylate cyclase activity in human tissues. Adenylate cyclase activity in liver, lung, pancreas, and heart ventricle, from full-term and premature newborns has been assayed. Of all tissues studied, heart had the highest activity. Fluoride 10 mM stimulates adenylate cyclase in all tissues. Glucagon evoked a small activation of liver adenylate cyclase.", "PMID": 974187}
{"id": "pubmed23n0032_9777", "title": "A study of the permeability of the guinea pig placenta to citrate using recirculating placental perfusion technique.", "content": "The role of the guinea pig placenta in fetal citrate metabolism has been studied by perfusing the placenta in situ. The initial citrate concentrations in the perfusate plasma were lower than in the maternal plasma but rose progressively until, in some cases, they exceeded those in the mother. The rise was uninfluenced by intravenous administration of citrate to the mother. Citrate therefore does not enter the perfusate by simple diffusion from the maternal compartment. It may be synthesised within the placenta. From the rate of accumulation of citrate in the perfusate, the supply to the fetus was calculated to be small compared to the fetal metabolic rate. Citrate added to the perfusate became distributed in a space similar to the extracellular space and no change occurred in maternal citrate concentrations. Thus, the placenta does not appear to metabolise citrate or allow transport to the mother.", "contents": "A study of the permeability of the guinea pig placenta to citrate using recirculating placental perfusion technique. The role of the guinea pig placenta in fetal citrate metabolism has been studied by perfusing the placenta in situ. The initial citrate concentrations in the perfusate plasma were lower than in the maternal plasma but rose progressively until, in some cases, they exceeded those in the mother. The rise was uninfluenced by intravenous administration of citrate to the mother. Citrate therefore does not enter the perfusate by simple diffusion from the maternal compartment. It may be synthesised within the placenta. From the rate of accumulation of citrate in the perfusate, the supply to the fetus was calculated to be small compared to the fetal metabolic rate. Citrate added to the perfusate became distributed in a space similar to the extracellular space and no change occurred in maternal citrate concentrations. Thus, the placenta does not appear to metabolise citrate or allow transport to the mother.", "PMID": 974188}
{"id": "pubmed23n0032_9778", "title": "Effect of diet on renal response to salt challenge in neonatal piglets.", "content": "In piglets 14-40 days of age, blood pressure and glomerular filtration rate increased significantly with age and were lower than those reported for adult pigs. There was no effect of dietary salt on the maturation of these parameters and on tubular sodium reabsorption, although the piglets on a low salt diet had lower inulin space than piglets receiving higher dietary salt. After the completion of a 24-hour salt challenge there was little evidence of sodium retention and there was no effect of previous dietary salt intake on sodium excretion. However, salt challenge resulted in higher blood pressure and elevated serum sodium only in piglets on a low sodium diet. These results suggest that in contrast to puppies and infants, the renal response to salt challenge is physiologically adequate in piglets at 14-40 days although glomerular function is still immature. Dietary salt history appears to have little effect on the degree of sodium excretion during salt challenge in this age range.", "contents": "Effect of diet on renal response to salt challenge in neonatal piglets. In piglets 14-40 days of age, blood pressure and glomerular filtration rate increased significantly with age and were lower than those reported for adult pigs. There was no effect of dietary salt on the maturation of these parameters and on tubular sodium reabsorption, although the piglets on a low salt diet had lower inulin space than piglets receiving higher dietary salt. After the completion of a 24-hour salt challenge there was little evidence of sodium retention and there was no effect of previous dietary salt intake on sodium excretion. However, salt challenge resulted in higher blood pressure and elevated serum sodium only in piglets on a low sodium diet. These results suggest that in contrast to puppies and infants, the renal response to salt challenge is physiologically adequate in piglets at 14-40 days although glomerular function is still immature. Dietary salt history appears to have little effect on the degree of sodium excretion during salt challenge in this age range.", "PMID": 974189}
{"id": "pubmed23n0032_9779", "title": "Indication of increase of the lecithin/sphingomyelin (L/S) ratio in lung fluid of lambs maternally treated with metabolite VIII of Bisolvon.", "content": "Metabolite VIII of Bisolvon (Met. VIII) was administered to six pregnant ewes (i.m. daily 5 or 10 mg/kg for 7-9 days). The treatment was started at the 116th/117th or 117th/118th day of gestation and in one case the drug was given to the fetus (i.v. 2 mg/ml/h). Fetal lung fluid obtained from chronically implanted catheters was used for the estimation of the lecithin/sphingomyelin ratio. The maternal treatment with Met. VIII resulted in a significantly increased growth of the L/S ratio compared to the control group (n=4). In three lambs a sharp increase of the L/S ratio was noticed during the last days of Met. VIII treatment. The time gap between first treatment and noticeable effect was 6-8 days. It is postulated that the noticed increase of L/S ratio is the consequence of Met. VIII treatment and very unlikely the result of a release of glucocorticoticoids.", "contents": "Indication of increase of the lecithin/sphingomyelin (L/S) ratio in lung fluid of lambs maternally treated with metabolite VIII of Bisolvon. Metabolite VIII of Bisolvon (Met. VIII) was administered to six pregnant ewes (i.m. daily 5 or 10 mg/kg for 7-9 days). The treatment was started at the 116th/117th or 117th/118th day of gestation and in one case the drug was given to the fetus (i.v. 2 mg/ml/h). Fetal lung fluid obtained from chronically implanted catheters was used for the estimation of the lecithin/sphingomyelin ratio. The maternal treatment with Met. VIII resulted in a significantly increased growth of the L/S ratio compared to the control group (n=4). In three lambs a sharp increase of the L/S ratio was noticed during the last days of Met. VIII treatment. The time gap between first treatment and noticeable effect was 6-8 days. It is postulated that the noticed increase of L/S ratio is the consequence of Met. VIII treatment and very unlikely the result of a release of glucocorticoticoids.", "PMID": 974190}
{"id": "pubmed23n0032_9780", "title": "Influence of oxytocin and meperidine on the isolated human umbilical artery.", "content": "The influence of oxytocin and meperidine on responses of the isolated umbilical artery to acetylcholine (ACh) and/or 5-hydroxytryptamine (5-HT) was investigated. 32 preparations from 21 normal full-term deliveries were utilized. The Bunce double-blade hemostat provided an excellent means to collect cord specimens with the least postpartal changes. A method of simultaneously measuring the resistance to flow and longitudinal tension in the perfused artery was found to be a sensitive way to register functional responses. Results showed that oxytocin enhanced the response of the umbilical artery to ACh and 5-HT while meperidine blocked the constriction produced by 5-HT. Both these effects lasted from 30 min to more than 2 h and were reversible.", "contents": "Influence of oxytocin and meperidine on the isolated human umbilical artery. The influence of oxytocin and meperidine on responses of the isolated umbilical artery to acetylcholine (ACh) and/or 5-hydroxytryptamine (5-HT) was investigated. 32 preparations from 21 normal full-term deliveries were utilized. The Bunce double-blade hemostat provided an excellent means to collect cord specimens with the least postpartal changes. A method of simultaneously measuring the resistance to flow and longitudinal tension in the perfused artery was found to be a sensitive way to register functional responses. Results showed that oxytocin enhanced the response of the umbilical artery to ACh and 5-HT while meperidine blocked the constriction produced by 5-HT. Both these effects lasted from 30 min to more than 2 h and were reversible.", "PMID": 974191}
{"id": "pubmed23n0032_9781", "title": "Liquid ventilation in immature newborn rabbits.", "content": "Rabbit fetuses delivered 3 days before term were artificially ventilated with air, oxygen, or oxygenated liquid fluorocarbon for 1.5-3 h. Oxygenation, as revealed by skin colour and ECG was superior in liquid-ventilated animals. Also, liquid ventilation was less harmful to the bronchiolar epithelium and, in contrast to ventilation with gas or oxygen, did not cause the formation of hyaline membranes.", "contents": "Liquid ventilation in immature newborn rabbits. Rabbit fetuses delivered 3 days before term were artificially ventilated with air, oxygen, or oxygenated liquid fluorocarbon for 1.5-3 h. Oxygenation, as revealed by skin colour and ECG was superior in liquid-ventilated animals. Also, liquid ventilation was less harmful to the bronchiolar epithelium and, in contrast to ventilation with gas or oxygen, did not cause the formation of hyaline membranes.", "PMID": 974192}
{"id": "pubmed23n0032_9782", "title": "Heterogeneity of pituitary and endogenous plasma human growth hormone from fetuses, premature and full-term newborns.", "content": "Fetal and adult human growth hormone (HGH) extract from human pituitaries as well as plasma from premature and full-term newborns and children were submitted to chromatography on Sephadex G-75 and the immunoreactive HGH (IRHGH) in the elution fractions was determined quantitatively by radioimmunoassay. Three fractions of IRHGH could be differentiated according to the elution pattern: 'big-big', 'big' and 'little' IRHGH. In fetal pituitary extract only 'big-big' and 'little' IRHGH were found, whereas in adult pituitary extract all three forms of IRHGH were present. In the plasma samples studied no 'big-big' form was found; only 'big' and 'little' IRHGH appeared. In addition, plasma samples of premature newborns had a smaller proportion of the 'big' IRHGH than did those of the full-term newborns and older children.", "contents": "Heterogeneity of pituitary and endogenous plasma human growth hormone from fetuses, premature and full-term newborns. Fetal and adult human growth hormone (HGH) extract from human pituitaries as well as plasma from premature and full-term newborns and children were submitted to chromatography on Sephadex G-75 and the immunoreactive HGH (IRHGH) in the elution fractions was determined quantitatively by radioimmunoassay. Three fractions of IRHGH could be differentiated according to the elution pattern: 'big-big', 'big' and 'little' IRHGH. In fetal pituitary extract only 'big-big' and 'little' IRHGH were found, whereas in adult pituitary extract all three forms of IRHGH were present. In the plasma samples studied no 'big-big' form was found; only 'big' and 'little' IRHGH appeared. In addition, plasma samples of premature newborns had a smaller proportion of the 'big' IRHGH than did those of the full-term newborns and older children.", "PMID": 974193}
{"id": "pubmed23n0032_9783", "title": "Precursor and end product effects upon indoleamine maturation in mouse brain.", "content": "The effects of tryptophan loading and the acid transport inhibitor, probenecid, on the maturation of 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) have been studied in the maturing mouse brain. Tryptophan loading elevates already high endogenous tryptophan at all stages studied. It does not alter 5-HT or 5-HIAA at day 1 but does elevate levels of both at later stages. Probenecid does not affect 5-HT at any age or 5-HIAA at day 1, but does cause increasingly greater elevations of 5-HIAA at later stages. The results indicate that tryptophan-5-hydroxylase is substrate saturated in early postnatal stages but not later, and that the early postnatal brain lacks an acid transport mechanism for 5-HIAA egress but develops this at later stages.", "contents": "Precursor and end product effects upon indoleamine maturation in mouse brain. The effects of tryptophan loading and the acid transport inhibitor, probenecid, on the maturation of 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) have been studied in the maturing mouse brain. Tryptophan loading elevates already high endogenous tryptophan at all stages studied. It does not alter 5-HT or 5-HIAA at day 1 but does elevate levels of both at later stages. Probenecid does not affect 5-HT at any age or 5-HIAA at day 1, but does cause increasingly greater elevations of 5-HIAA at later stages. The results indicate that tryptophan-5-hydroxylase is substrate saturated in early postnatal stages but not later, and that the early postnatal brain lacks an acid transport mechanism for 5-HIAA egress but develops this at later stages.", "PMID": 974194}
{"id": "pubmed23n0032_9784", "title": "Thyroid hormone and cell formation in the developing rat cerebellum.", "content": "Effects of neonatal hyperthyroidism on cell formation in the developing rat cerebellum were reinvestigated. Administration at birth of excessive doses of thyroxine or triiodothyronine led to an early stimulation of cell acquisition, followed by a permanent deficit of cells in the cerebellum. The corrective effects of physiological doses of thyroxine on the troubles of the histological and biochemical development of the cerebellum in thyroid-deficient animals were also studied. As early as 6 days, cell maturation and formation were already retarded in animals treated with propylthiouracil, but, as previously reported, cell formation was prolonged and the final number of cells was normal. Administration to thyroid-deficient animals of progressively increasing doses of thyroxine, nearly equal to the amounts of hormone secreted by the thyroid gland of the developing normal rat, returned the evolution of the cerebellar wet weight and of the cerebellar DNA to normal, as well as the histological maturation of the cerebellum, even if it did not entirely correct the retardation of body growth. These results are consistent with the view that thyroid hormone early stimulates maturation of the cerebellar germinative cells and subsequently interacts with cell formation in the cerebellum, and that this action is physiological.", "contents": "Thyroid hormone and cell formation in the developing rat cerebellum. Effects of neonatal hyperthyroidism on cell formation in the developing rat cerebellum were reinvestigated. Administration at birth of excessive doses of thyroxine or triiodothyronine led to an early stimulation of cell acquisition, followed by a permanent deficit of cells in the cerebellum. The corrective effects of physiological doses of thyroxine on the troubles of the histological and biochemical development of the cerebellum in thyroid-deficient animals were also studied. As early as 6 days, cell maturation and formation were already retarded in animals treated with propylthiouracil, but, as previously reported, cell formation was prolonged and the final number of cells was normal. Administration to thyroid-deficient animals of progressively increasing doses of thyroxine, nearly equal to the amounts of hormone secreted by the thyroid gland of the developing normal rat, returned the evolution of the cerebellar wet weight and of the cerebellar DNA to normal, as well as the histological maturation of the cerebellum, even if it did not entirely correct the retardation of body growth. These results are consistent with the view that thyroid hormone early stimulates maturation of the cerebellar germinative cells and subsequently interacts with cell formation in the cerebellum, and that this action is physiological.", "PMID": 974195}
{"id": "pubmed23n0032_9785", "title": "Medical devices as medical problems: the regulation of devices for clinical use.", "content": "The development of statutes to regulate the safety and efficacy of medical devices has been proceeding rapidly. The protection of the consumer from dangerous and ineffective devices is obviously necessary and must be carried out rapidly and efficiently. The electrical shock hazard in prevalence and violence is a case in point. There is no question that many devices are not well made and not suited for the function for which they are sold. These must be controlled, but at the same time we must be careful not to destroy the private research and development efforts which are producing reliable instrumentation through over control and regulation.", "contents": "Medical devices as medical problems: the regulation of devices for clinical use. The development of statutes to regulate the safety and efficacy of medical devices has been proceeding rapidly. The protection of the consumer from dangerous and ineffective devices is obviously necessary and must be carried out rapidly and efficiently. The electrical shock hazard in prevalence and violence is a case in point. There is no question that many devices are not well made and not suited for the function for which they are sold. These must be controlled, but at the same time we must be careful not to destroy the private research and development efforts which are producing reliable instrumentation through over control and regulation.", "PMID": 974209}
{"id": "pubmed23n0032_9786", "title": "An endoscopic carrier with complete directional control: design principles.", "content": "We describe a carrier for instruments used in endoscopy. It consists of a train of articulated segments whose angular displacement is controlled by a series of magnetic clutches. The operator guides the leading segment under view from the carrier's proximal end, and the guide angle is repeated automatically at the articulations of all following segments. This mechanism is used to lead the entire length of the carrier around curves or flexures. A central channel accepts instruments for observation and manipulation. We believe that endoscopic examinations particularly of the upper and the lower digestive tracts will become much easier to perform with this new endoscopic sonde.", "contents": "An endoscopic carrier with complete directional control: design principles. We describe a carrier for instruments used in endoscopy. It consists of a train of articulated segments whose angular displacement is controlled by a series of magnetic clutches. The operator guides the leading segment under view from the carrier's proximal end, and the guide angle is repeated automatically at the articulations of all following segments. This mechanism is used to lead the entire length of the carrier around curves or flexures. A central channel accepts instruments for observation and manipulation. We believe that endoscopic examinations particularly of the upper and the lower digestive tracts will become much easier to perform with this new endoscopic sonde.", "PMID": 974210}
{"id": "pubmed23n0032_9787", "title": "An elbow orthosis.", "content": "An elbow orthosis for unilaterally handicapped persons has been developed. The operating principles and construction of the orthosis are described and its important parameters are determined. The procedure of parameter adjustment is given in detail and the area of application is indicated. Preliminary clinical results are discussed.", "contents": "An elbow orthosis. An elbow orthosis for unilaterally handicapped persons has been developed. The operating principles and construction of the orthosis are described and its important parameters are determined. The procedure of parameter adjustment is given in detail and the area of application is indicated. Preliminary clinical results are discussed.", "PMID": 974211}
{"id": "pubmed23n0032_9788", "title": "The measurement of urethral resistance and mural tension using simple programmable modules.", "content": "The theory of urethral resistance and the derivation or mural tension have been discussed. A method for the continuous calculation and presentation of these parameters has been described. Urethral resistance and mural tensions are altered by the presence of bladder outflow obstruction and have been shown to return to normality after the obstruction is removed.", "contents": "The measurement of urethral resistance and mural tension using simple programmable modules. The theory of urethral resistance and the derivation or mural tension have been discussed. A method for the continuous calculation and presentation of these parameters has been described. Urethral resistance and mural tensions are altered by the presence of bladder outflow obstruction and have been shown to return to normality after the obstruction is removed.", "PMID": 974212}
{"id": "pubmed23n0032_9789", "title": "A possible new sorbent dialyser for haemodialysis.", "content": "A system is proposed for haemodialysis which involves both dialysis and adsorption. The blood compartment is separated by a membrane from a compartment containing adsorbents and ion exchangers under reduced pressure. Urea is broken down by urease adsorbed on an ion exchanger. The ammonia produced is pumped out and the rest of the unwanted substances are bound to the adsorbing materials. The geometry and principles governing the operation of the device obviate the use of circulating dialysate fluid. Preliminary results from in vitro experiments with such a system are reported and the possibility of using this system clinically is discussed.", "contents": "A possible new sorbent dialyser for haemodialysis. A system is proposed for haemodialysis which involves both dialysis and adsorption. The blood compartment is separated by a membrane from a compartment containing adsorbents and ion exchangers under reduced pressure. Urea is broken down by urease adsorbed on an ion exchanger. The ammonia produced is pumped out and the rest of the unwanted substances are bound to the adsorbing materials. The geometry and principles governing the operation of the device obviate the use of circulating dialysate fluid. Preliminary results from in vitro experiments with such a system are reported and the possibility of using this system clinically is discussed.", "PMID": 974213}
{"id": "pubmed23n0032_9790", "title": "Patient monitoring in the operating theatre.", "content": "Anaesthetised patients are monitored to ensure their safety. Simple clinical observations must not be replaced by electronic instruments--these provide an extension of the clinical senses. The choice of parameters for monitoring is discussed. The design of the Ninewells main operating theatre suite is described. An 8-channel bourne in the base of the theatre table conveys patient signals to a 4-channel recorder in a monitoring laboratory. Outputs are displayed on a wall mounted display in theatre. Two-way speech intercommunication exists with monitoring technician and students.", "contents": "Patient monitoring in the operating theatre. Anaesthetised patients are monitored to ensure their safety. Simple clinical observations must not be replaced by electronic instruments--these provide an extension of the clinical senses. The choice of parameters for monitoring is discussed. The design of the Ninewells main operating theatre suite is described. An 8-channel bourne in the base of the theatre table conveys patient signals to a 4-channel recorder in a monitoring laboratory. Outputs are displayed on a wall mounted display in theatre. Two-way speech intercommunication exists with monitoring technician and students.", "PMID": 974216}
{"id": "pubmed23n0032_9791", "title": "A prosthetic vas deferens.", "content": "Six dogs were implanted with the synthetic vas deferens device as part of our continuing research into the development of a reversible occlusive device for implant in the transected vas deferens. The experimental objectives were to assess the effects of sperm path length and duration of aspermia on total sperm count and motility by implanting three different lengths of the device in animals with different degrees of aspermic duration induced by bilateral vasectomy. Device lengths varied from 1.55 cm to 4.3 cm including the pilot tubes. The prosthesis is fabricated entirely of silicone rubber with Dacron velour tissue ingrowth material bonded to the outside surface of the ring to which vas ends are sutured. The pilot tubes extend 4 mm beyond the suture rings and are 0.6 mm OD with a 0.3 mm ID that extends through the entire length of the device. The flexible pilot tubes are an important feature of this design and insert into the vas lumen ensuring alignment between the device and the vas deferens while maintaining the necessary resiliency to adapt to the inherent movements of the vas without perforation. All animals included in these experiments were carefully selected and conditioned before implant. The evaluation of performance was based on semen samples collected at one week intervals. Sperm subsequently appeared in the ejaculate of all 6 dogs, demonstrating that the restoration of sperm transport can be restored after bilateral vasectomy through the implantation of a prosthetic vas deferens. The potential application of this device to the human male for the restoration of fertility appears excellent.", "contents": "A prosthetic vas deferens. Six dogs were implanted with the synthetic vas deferens device as part of our continuing research into the development of a reversible occlusive device for implant in the transected vas deferens. The experimental objectives were to assess the effects of sperm path length and duration of aspermia on total sperm count and motility by implanting three different lengths of the device in animals with different degrees of aspermic duration induced by bilateral vasectomy. Device lengths varied from 1.55 cm to 4.3 cm including the pilot tubes. The prosthesis is fabricated entirely of silicone rubber with Dacron velour tissue ingrowth material bonded to the outside surface of the ring to which vas ends are sutured. The pilot tubes extend 4 mm beyond the suture rings and are 0.6 mm OD with a 0.3 mm ID that extends through the entire length of the device. The flexible pilot tubes are an important feature of this design and insert into the vas lumen ensuring alignment between the device and the vas deferens while maintaining the necessary resiliency to adapt to the inherent movements of the vas without perforation. All animals included in these experiments were carefully selected and conditioned before implant. The evaluation of performance was based on semen samples collected at one week intervals. Sperm subsequently appeared in the ejaculate of all 6 dogs, demonstrating that the restoration of sperm transport can be restored after bilateral vasectomy through the implantation of a prosthetic vas deferens. The potential application of this device to the human male for the restoration of fertility appears excellent.", "PMID": 974217}
{"id": "pubmed23n0032_9792", "title": "Concentrating engines and the kidney. III. Canonical mass balance equation for multinephron models of the renal medulla.", "content": "The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or loops of Henle extend to the papilla. It is shown that if descending limbs of Henle and descending vasa recta enter the medulla at approximately plasma osmolality, the concentration ratio is given by: r = 1/[1 - ft(1 - fu)(1 - fw)], where ft is fractional solute transport out of ascending Henle's limb, fu is fractional urine flow, and fw is fractional dissipation; fw is a measure of the solute returned to the systemic circulation without its isotonic complement of water. A modified equation that applies to the diluting as well as the concentrating kidney is also derived. By allowing concentrations in interstitium and vascular capillaries to become identical at a given medullary level, conservation relations are derived for a multinephron central core model of the renal medulla.", "contents": "Concentrating engines and the kidney. III. Canonical mass balance equation for multinephron models of the renal medulla. The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or loops of Henle extend to the papilla. It is shown that if descending limbs of Henle and descending vasa recta enter the medulla at approximately plasma osmolality, the concentration ratio is given by: r = 1/[1 - ft(1 - fu)(1 - fw)], where ft is fractional solute transport out of ascending Henle's limb, fu is fractional urine flow, and fw is fractional dissipation; fw is a measure of the solute returned to the systemic circulation without its isotonic complement of water. A modified equation that applies to the diluting as well as the concentrating kidney is also derived. By allowing concentrations in interstitium and vascular capillaries to become identical at a given medullary level, conservation relations are derived for a multinephron central core model of the renal medulla.", "PMID": 974220}
{"id": "pubmed23n0032_9793", "title": "Lecithin translational diffusion studied by pulsed nuclear magnetic resonance.", "content": "The translational diffusion coefficient of egg yolk and dilauroyl lecithin in optically isotropic phases containing sodium cholate has been measured using the pulsed NMR magnetic field gradient method. After a correction for geometrical factors the measured diffusion coefficient is found to agree well with previous determinations in phospholipid systems. The experimental data imply that the cubic mesophase of the lecithin-sodium cholate-water system contains continuous lipid aggregates. A possible model of the arrangement of the different amphiphile molecules in the cubic phase is discussed.", "contents": "Lecithin translational diffusion studied by pulsed nuclear magnetic resonance. The translational diffusion coefficient of egg yolk and dilauroyl lecithin in optically isotropic phases containing sodium cholate has been measured using the pulsed NMR magnetic field gradient method. After a correction for geometrical factors the measured diffusion coefficient is found to agree well with previous determinations in phospholipid systems. The experimental data imply that the cubic mesophase of the lecithin-sodium cholate-water system contains continuous lipid aggregates. A possible model of the arrangement of the different amphiphile molecules in the cubic phase is discussed.", "PMID": 974221}
{"id": "pubmed23n0032_9794", "title": "The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. III. Characterization of spectral parameters.", "content": "The variations of optical density and fluorescence of lucensomycin are good indices of the binding of this polyenic antibiotic to membranes. The former parameter reflects more generally the binding to any site present in the membrane, while the latter is more specific for binding to cholesterol. The chromophore of the lucensomycin-cholesterol complex has a relatively long lifetime, is almost immobile in the membrane, and is not accessible to water-soluble fluorescence-quenching agents. The stoichiometry, evaluated fluorometrically, corresponds to about two cholesterol molecules per polyene. In colloidal cholesterol suspensions, the extent of binding as a function of free polyene concentration is described by rectangular hyperbolae, the dissociation constant being, however, dependent on the sterol concentration. In erythrocyte membranes, on the other hand, and even more markedly in model systems containing appropriate solvents, the combination between lucensomycin and the sterol sites is described by sigmoid titration curves, indicative of cooperative effects, and probably due to solvation of cholesterol.", "contents": "The interaction of the polyene antibiotic lucensomycin with cholesterol in erythrocyte membranes and in model systems. III. Characterization of spectral parameters. The variations of optical density and fluorescence of lucensomycin are good indices of the binding of this polyenic antibiotic to membranes. The former parameter reflects more generally the binding to any site present in the membrane, while the latter is more specific for binding to cholesterol. The chromophore of the lucensomycin-cholesterol complex has a relatively long lifetime, is almost immobile in the membrane, and is not accessible to water-soluble fluorescence-quenching agents. The stoichiometry, evaluated fluorometrically, corresponds to about two cholesterol molecules per polyene. In colloidal cholesterol suspensions, the extent of binding as a function of free polyene concentration is described by rectangular hyperbolae, the dissociation constant being, however, dependent on the sterol concentration. In erythrocyte membranes, on the other hand, and even more markedly in model systems containing appropriate solvents, the combination between lucensomycin and the sterol sites is described by sigmoid titration curves, indicative of cooperative effects, and probably due to solvation of cholesterol.", "PMID": 974222}
{"id": "pubmed23n0032_9795", "title": "Dynamics of fluorescence marker concentration as a probe of mobility.", "content": "We have developed an effective experimental system for the characterization of molecular and structural mobility. It incorporates a modified fluorescence microscope geometry and a variety of analytical techniques to measure effective diffusion coefficients ranging over almost six orders of magnitude, from less than 10(-11) cm2/s to greater than 10(-6) cm2/s. Two principal techniques, fluorescence correlation spectroscopy (FCS) and fluorescence photobleaching recovery (FPR), are employed. In the FPR technique, translational transport rates are measured by monitoring the evolution of a spatial inhomogeneity of fluorescence that is produced photochemically in a microscopic volume by a short burst of intense laser radiation. In contrast, FCS uses laser-induced fluorescence to probe the spontaneous concentration fluctuations in microscopic sample volumes. The kinetics are analyzed by computing time-correlation functions of the stochastic fluctuations of the measured fluorescence intensity. The optical system and digital photocount correlator designed around a dedicated minicomputer are described and discussed. The general power of these techniques is demonstrated with examples from studies conducted on bulk solutions, lipid bilayer membranes, and mammalian cell plasma membranes.", "contents": "Dynamics of fluorescence marker concentration as a probe of mobility. We have developed an effective experimental system for the characterization of molecular and structural mobility. It incorporates a modified fluorescence microscope geometry and a variety of analytical techniques to measure effective diffusion coefficients ranging over almost six orders of magnitude, from less than 10(-11) cm2/s to greater than 10(-6) cm2/s. Two principal techniques, fluorescence correlation spectroscopy (FCS) and fluorescence photobleaching recovery (FPR), are employed. In the FPR technique, translational transport rates are measured by monitoring the evolution of a spatial inhomogeneity of fluorescence that is produced photochemically in a microscopic volume by a short burst of intense laser radiation. In contrast, FCS uses laser-induced fluorescence to probe the spontaneous concentration fluctuations in microscopic sample volumes. The kinetics are analyzed by computing time-correlation functions of the stochastic fluctuations of the measured fluorescence intensity. The optical system and digital photocount correlator designed around a dedicated minicomputer are described and discussed. The general power of these techniques is demonstrated with examples from studies conducted on bulk solutions, lipid bilayer membranes, and mammalian cell plasma membranes.", "PMID": 974223}
{"id": "pubmed23n0032_9796", "title": "Effects of removing extracellular Ca2+ on excitation and adaptation in Limulus ventral photoreceptors.", "content": "In Limulus ventral photoreceptors, removing extracellular calcium (Ca2+o) increases the median latency of light-evoked discrete waves. Removal greatly lengthens the time-to-peak of responses in the dark-adapted cell, but not in the light-adapted cell. Removal does not block light-adaptation or the light-induced rise in intracellular calcium (Ca2+i). These results are interpreted in terms of the hypothesis that both sensitivity and the kinetics of excitation are dependent on Ca2+i, and that Ca2+i is dependent on Ca2+o in the dark-adapted cell, but in the light is dependent largely on Ca2+ released from intracellular compartments.", "contents": "Effects of removing extracellular Ca2+ on excitation and adaptation in Limulus ventral photoreceptors. In Limulus ventral photoreceptors, removing extracellular calcium (Ca2+o) increases the median latency of light-evoked discrete waves. Removal greatly lengthens the time-to-peak of responses in the dark-adapted cell, but not in the light-adapted cell. Removal does not block light-adaptation or the light-induced rise in intracellular calcium (Ca2+i). These results are interpreted in terms of the hypothesis that both sensitivity and the kinetics of excitation are dependent on Ca2+i, and that Ca2+i is dependent on Ca2+o in the dark-adapted cell, but in the light is dependent largely on Ca2+ released from intracellular compartments.", "PMID": 974224}
{"id": "pubmed23n0032_9797", "title": "Binding isotherms and the interaction between proflavin and a DNA of high G-C content.", "content": "Binding isotherms corresponding to several situations of ligand binding to a linear polymer are calculated, including cases of cooperativity or anticooperativity between the bound ligand states, multiple binding modes that are competitive or non competitive, and possible exclusive of an arbitrary number of adjacent sites upon occupancy of a site by a single ligand. The sequence generating function method of Lifson and Bradley is used, requiring the assumption that no end effects are involved. The case of strong binding of the dye proflavin to a DNA of high G-C content, that of M. lysodeikticus, is considered in detail, and a single model capable of reconciling the available kinetic and equilibrium data on this system, involving two competing binding modes, is discussed.", "contents": "Binding isotherms and the interaction between proflavin and a DNA of high G-C content. Binding isotherms corresponding to several situations of ligand binding to a linear polymer are calculated, including cases of cooperativity or anticooperativity between the bound ligand states, multiple binding modes that are competitive or non competitive, and possible exclusive of an arbitrary number of adjacent sites upon occupancy of a site by a single ligand. The sequence generating function method of Lifson and Bradley is used, requiring the assumption that no end effects are involved. The case of strong binding of the dye proflavin to a DNA of high G-C content, that of M. lysodeikticus, is considered in detail, and a single model capable of reconciling the available kinetic and equilibrium data on this system, involving two competing binding modes, is discussed.", "PMID": 974225}
{"id": "pubmed23n0032_9798", "title": "Scanning gel chromatography: determination of transport parameters from dynamic profiles measured at low solute concentration.", "content": "Direct optical scanning of solute boundaries in large zone gel chromatography experiments provides an accurate means of determining boundary profile shapes and rates of motion. A method has been developed for correcting such boundaries to a constant time frame, eliminating the distortion which arises from finite column scanning rate. Centroids of the corrected profiles can be used to determine the partition cross section for the solute of interest. The partition cross section and flow rate determine translational motion within the column. The axial dispersion coefficient, L, which characterizes rate of boundary spreading may also be calculated from the profiles. In order to explore these procedures a study of four noninteracting solutes was conducted. Partition cross sections determined from rates of motion of boundary centroids were found to be in good agreement with those determined by the equilibrium saturation method on the same column. In order to explore the lowest concentration limits of the technique and to illustrate the boundary characteristics for a self-associating solute, a study of carboxyhemoglobin was conducted over a wide concentration range. From measurements at 220 nm the lowest concentration where useful data could be obtained was 2 micrograms per ml (0.12muM heme). These results establish validity of the procedures used in analyzing the rates of boundary transport and in studying solute transport over a wide range of conditions.", "contents": "Scanning gel chromatography: determination of transport parameters from dynamic profiles measured at low solute concentration. Direct optical scanning of solute boundaries in large zone gel chromatography experiments provides an accurate means of determining boundary profile shapes and rates of motion. A method has been developed for correcting such boundaries to a constant time frame, eliminating the distortion which arises from finite column scanning rate. Centroids of the corrected profiles can be used to determine the partition cross section for the solute of interest. The partition cross section and flow rate determine translational motion within the column. The axial dispersion coefficient, L, which characterizes rate of boundary spreading may also be calculated from the profiles. In order to explore these procedures a study of four noninteracting solutes was conducted. Partition cross sections determined from rates of motion of boundary centroids were found to be in good agreement with those determined by the equilibrium saturation method on the same column. In order to explore the lowest concentration limits of the technique and to illustrate the boundary characteristics for a self-associating solute, a study of carboxyhemoglobin was conducted over a wide concentration range. From measurements at 220 nm the lowest concentration where useful data could be obtained was 2 micrograms per ml (0.12muM heme). These results establish validity of the procedures used in analyzing the rates of boundary transport and in studying solute transport over a wide range of conditions.", "PMID": 974226}
{"id": "pubmed23n0032_9799", "title": "Active enzyme gel chromatography: II. Computer simulations.", "content": "The behavior of an enzyme undergoing reaction while on a gel chromatography column has been studied by computer simulation using the steady state assumtion for a system with a single enzyme-substrate complex. The profiles of the enzyme-substrate complex, product, and substrate were examined varying the parameters of kcat, flow rate, partition coefficient dispersion, and time. These investigations confirm that much information about both the active enzyme and the product may be obtained by examining the product profile alone, varying the power of applying scanning gel chromatography to active enzyme systems.", "contents": "Active enzyme gel chromatography: II. Computer simulations. The behavior of an enzyme undergoing reaction while on a gel chromatography column has been studied by computer simulation using the steady state assumtion for a system with a single enzyme-substrate complex. The profiles of the enzyme-substrate complex, product, and substrate were examined varying the parameters of kcat, flow rate, partition coefficient dispersion, and time. These investigations confirm that much information about both the active enzyme and the product may be obtained by examining the product profile alone, varying the power of applying scanning gel chromatography to active enzyme systems.", "PMID": 974227}
{"id": "pubmed23n0032_9800", "title": "Determining the base sequence of RNA molecules using continuous degradation kinetics.", "content": "A kinetic approach to sequence analysis is presented. A polymer chain composed of a small number of monomer types is degraded continuously with degradation proceeding in only one direction of the chain. Ordinarily, the direct determination of the sequence from the amount of degraded monomers is hampered by the rapid loss of reaction synchrony. However, it is shown, that if the monomers can be measured reasonably accurately, one can solve a system of linear equations with the unknowns giving the positions of the monomers in the chain, and the syn chrony loss due to the stochastic nature of the degradation process can be accounted for. Model calculations are presented.", "contents": "Determining the base sequence of RNA molecules using continuous degradation kinetics. A kinetic approach to sequence analysis is presented. A polymer chain composed of a small number of monomer types is degraded continuously with degradation proceeding in only one direction of the chain. Ordinarily, the direct determination of the sequence from the amount of degraded monomers is hampered by the rapid loss of reaction synchrony. However, it is shown, that if the monomers can be measured reasonably accurately, one can solve a system of linear equations with the unknowns giving the positions of the monomers in the chain, and the syn chrony loss due to the stochastic nature of the degradation process can be accounted for. Model calculations are presented.", "PMID": 974228}
{"id": "pubmed23n0032_9801", "title": "Rheology of fibrin clots. III. Shear creep and creep recovery of fine ligated and coarse unligated closts.", "content": "Creep and creep recovery of human fibrin clots in small shearing deformations have been investigated over a time scale from 24 to 10(4) s. Coarse, unligated clots and fine clots ligated by fibrinoligase in the presence of calcium ions were studied to suppliement previous data on coarse ligated and fine unligated clots. Stress was found to be proportional to strain up to at least a maximum shear strain (in torsion geometry) of 6.2%. The initial modulus (25 s after imposition of stress) is proportional to approximately the 1.5 power of concentration for fine ligated and coarse unligated clots. For fine unligated closts there is comparatively little creep subsequent to the initial deformation; ligation (in this case involving mostly the gamma chains) reduces the creep to nearly zero. For coarse unligated clots, there is substantially more creep under constant stress, and creep recovery is not complete. Ligation (in this case involving both camma and alpha chains) alrgely supresses the creep and causes the recovery to be complete. If the structure if fully formed before creep begins, tests of creep recovery by the Boltzmann superposition principle show adherence to linear visoelastic behavior for all four clot types. Otherwise, the Boltzmann test fails and the recovery is much less than calculated. For fine ligated clots, the observed recovery agrees well with that calculated on the basis of a dual structure model in which an additional independent structure is built up in the deformed state, so that the state of ease after removal of stress is a balance between two structures deformed in opposite senses. It is postulated that the coherence and elastic modulus of the fine ligated clot are largely due to steric blocking of long protofibrils with a high flexural stiffness. In the coarse clot, it is proposed that the structure involves extensive branching of thick bundles of protofibrils, which become permanently secured by the ligation of the alpha chains of the fibrin.", "contents": "Rheology of fibrin clots. III. Shear creep and creep recovery of fine ligated and coarse unligated closts. Creep and creep recovery of human fibrin clots in small shearing deformations have been investigated over a time scale from 24 to 10(4) s. Coarse, unligated clots and fine clots ligated by fibrinoligase in the presence of calcium ions were studied to suppliement previous data on coarse ligated and fine unligated clots. Stress was found to be proportional to strain up to at least a maximum shear strain (in torsion geometry) of 6.2%. The initial modulus (25 s after imposition of stress) is proportional to approximately the 1.5 power of concentration for fine ligated and coarse unligated clots. For fine unligated closts there is comparatively little creep subsequent to the initial deformation; ligation (in this case involving mostly the gamma chains) reduces the creep to nearly zero. For coarse unligated clots, there is substantially more creep under constant stress, and creep recovery is not complete. Ligation (in this case involving both camma and alpha chains) alrgely supresses the creep and causes the recovery to be complete. If the structure if fully formed before creep begins, tests of creep recovery by the Boltzmann superposition principle show adherence to linear visoelastic behavior for all four clot types. Otherwise, the Boltzmann test fails and the recovery is much less than calculated. For fine ligated clots, the observed recovery agrees well with that calculated on the basis of a dual structure model in which an additional independent structure is built up in the deformed state, so that the state of ease after removal of stress is a balance between two structures deformed in opposite senses. It is postulated that the coherence and elastic modulus of the fine ligated clot are largely due to steric blocking of long protofibrils with a high flexural stiffness. In the coarse clot, it is proposed that the structure involves extensive branching of thick bundles of protofibrils, which become permanently secured by the ligation of the alpha chains of the fibrin.", "PMID": 974229}
{"id": "pubmed23n0032_9802", "title": "The molecular mechanism of temperature enhancement of proton magnetic relaxation rates in methaemoprotein solutions. III. The effect of sixth ligand in high-spin derivatives.", "content": "The distinctions of the solvent-proton longitudinal magnetic relaxation (PMR) mechanisms between high-spin ferric aquo and fluoro complexes of some haemoproteins are discussed here. It becomes apparent that the \"transition\" from the exchange-limited PMR to the fast-exchange PMR mechanism upon addition of fluoride to some of the aquocomplexes is due to a more intense solvent-dynamics in the vicinity of the paramagnetic haem in the fluoromet derivatives. The can be rationalized by a conformational change induced by the fluoride ion, an effect not observed by X-ray analysis thus far. A possible mechanism of this change is indicated here.", "contents": "The molecular mechanism of temperature enhancement of proton magnetic relaxation rates in methaemoprotein solutions. III. The effect of sixth ligand in high-spin derivatives. The distinctions of the solvent-proton longitudinal magnetic relaxation (PMR) mechanisms between high-spin ferric aquo and fluoro complexes of some haemoproteins are discussed here. It becomes apparent that the \"transition\" from the exchange-limited PMR to the fast-exchange PMR mechanism upon addition of fluoride to some of the aquocomplexes is due to a more intense solvent-dynamics in the vicinity of the paramagnetic haem in the fluoromet derivatives. The can be rationalized by a conformational change induced by the fluoride ion, an effect not observed by X-ray analysis thus far. A possible mechanism of this change is indicated here.", "PMID": 974231}
{"id": "pubmed23n0032_9803", "title": "Chemical ionization and high resolution electron impact mass spectra of 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide and three of its metabolites.", "content": "The electron impact and the hydrogen and methane chemical ionization mass spectra of 5,6-dihydro-2-methyl-1,4-oxathin-3-carboxanilide, the sulfoxide and sulfone derived therefrom, and 2-(2-hydroxyethylthio)-acetoacetanilide enol have been determined. All four compounds show abundant molecular ions in the electron impact spectra and abundant [MH]+ ions in the methane chemical ionization spectra (along with the expected [M + C2H5]+ and [M + C3H5]+ ions), but relatively low [MH]+ ion signals in the hydrogen chemical ionization spectra. From high resolution mass measurements and metastable transitions determined by metastable ion refocusing, electron impact fragmentation mechanisms have been established. For 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide, 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4-oxide and 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4,4-dioxide the major fragmentation mode involves loss of the anilino radical from [M]+, followed by loss of C2H4. Fragmentation to form the aniline molecular ion increases in importance with increasing oxidation state of the sulfur. In the chemical ionization of these three compounds fragmentation of [MH]+ proceeds in a similar fashion by loss of neutral aniline and by formation of protonated aniline. The electron impact mass spectrum of 2-(2-hydroxyethylthio)acetoacetanilide is dominated by the molecular ion and the aniline molecular ion. However, in the chemical ionization mass spectra characteristic fragment ions involving loss of water and loss of aniline from [MH]+, as well as protonated aniline, are observed and serve to characterize the compound.", "contents": "Chemical ionization and high resolution electron impact mass spectra of 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide and three of its metabolites. The electron impact and the hydrogen and methane chemical ionization mass spectra of 5,6-dihydro-2-methyl-1,4-oxathin-3-carboxanilide, the sulfoxide and sulfone derived therefrom, and 2-(2-hydroxyethylthio)-acetoacetanilide enol have been determined. All four compounds show abundant molecular ions in the electron impact spectra and abundant [MH]+ ions in the methane chemical ionization spectra (along with the expected [M + C2H5]+ and [M + C3H5]+ ions), but relatively low [MH]+ ion signals in the hydrogen chemical ionization spectra. From high resolution mass measurements and metastable transitions determined by metastable ion refocusing, electron impact fragmentation mechanisms have been established. For 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide, 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4-oxide and 5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4,4-dioxide the major fragmentation mode involves loss of the anilino radical from [M]+, followed by loss of C2H4. Fragmentation to form the aniline molecular ion increases in importance with increasing oxidation state of the sulfur. In the chemical ionization of these three compounds fragmentation of [MH]+ proceeds in a similar fashion by loss of neutral aniline and by formation of protonated aniline. The electron impact mass spectrum of 2-(2-hydroxyethylthio)acetoacetanilide is dominated by the molecular ion and the aniline molecular ion. However, in the chemical ionization mass spectra characteristic fragment ions involving loss of water and loss of aniline from [MH]+, as well as protonated aniline, are observed and serve to characterize the compound.", "PMID": 974232}
{"id": "pubmed23n0032_9804", "title": "Field desorption mass spectrum of glucagon.", "content": "A low resolution, high sensitivity study has been made of the field desorption mass spectrum of glucagon, a 29 amino acid residue underivatized peptide. The highest molecular weight fragments observed, when spectra were taken under conditions which minimized surface reactions of the peptide on the emitter, were found to contain no more than 18 amino acid residues. The most probable fragmentation processes for ions larger than m/e 600 were the production of fragments containing 10, 11 or 12 amino acid residues.", "contents": "Field desorption mass spectrum of glucagon. A low resolution, high sensitivity study has been made of the field desorption mass spectrum of glucagon, a 29 amino acid residue underivatized peptide. The highest molecular weight fragments observed, when spectra were taken under conditions which minimized surface reactions of the peptide on the emitter, were found to contain no more than 18 amino acid residues. The most probable fragmentation processes for ions larger than m/e 600 were the production of fragments containing 10, 11 or 12 amino acid residues.", "PMID": 974233}
{"id": "pubmed23n0032_9805", "title": "Selected ion monitoring assay for the bronchodilator pirbuterol.", "content": "A selected ion monitoring assay has been developed to measure plasma levels of the bronchodilator pirbuterol in animals and man. Pirbuterol is extracted from plasma with acetone + potassium carbonate and after further purification is converted to its trimethylacetyl derivative. With the use of the stable isotope labeled internal standard, pirbuterol-d9, precisions of 10% and 6% were achieved at plasma concentrations of 1 and 20 ng ml-1, respectively. The synthesis of the deuterium labeled internal standard is described.", "contents": "Selected ion monitoring assay for the bronchodilator pirbuterol. A selected ion monitoring assay has been developed to measure plasma levels of the bronchodilator pirbuterol in animals and man. Pirbuterol is extracted from plasma with acetone + potassium carbonate and after further purification is converted to its trimethylacetyl derivative. With the use of the stable isotope labeled internal standard, pirbuterol-d9, precisions of 10% and 6% were achieved at plasma concentrations of 1 and 20 ng ml-1, respectively. The synthesis of the deuterium labeled internal standard is described.", "PMID": 974234}
{"id": "pubmed23n0032_9806", "title": "Simultaneous estimation of propoxyphene-do and propoxyphene-d2 levels by quantitative mass fragmentography. Potential utility in multidose investigations.", "content": "A quantitative mass fragmentographic method for the simultaneous determination of labeled and unlabeled propoxyphene in plasma is described. Dogs treated daily with propoxyphene-do were treated with a pulse dose of propoxyphene-d2 at day 20. It was found that following an initial rapid equilibrium phase levels of propoxyphene-d2 fell more rapidly than those of propoxyphene-do. This result suggests that 'deep' pools of tissue bound propoxyphene exist which exchange very slowly with drug present in the central compartment. Experimental evidence is presented which demonstrates that the difference in behavior of propoxyphene-do and -d2 is not due to unanticipated secondary isotope effects.", "contents": "Simultaneous estimation of propoxyphene-do and propoxyphene-d2 levels by quantitative mass fragmentography. Potential utility in multidose investigations. A quantitative mass fragmentographic method for the simultaneous determination of labeled and unlabeled propoxyphene in plasma is described. Dogs treated daily with propoxyphene-do were treated with a pulse dose of propoxyphene-d2 at day 20. It was found that following an initial rapid equilibrium phase levels of propoxyphene-d2 fell more rapidly than those of propoxyphene-do. This result suggests that 'deep' pools of tissue bound propoxyphene exist which exchange very slowly with drug present in the central compartment. Experimental evidence is presented which demonstrates that the difference in behavior of propoxyphene-do and -d2 is not due to unanticipated secondary isotope effects.", "PMID": 974235}
{"id": "pubmed23n0032_9807", "title": "Propoxyphene: pathways of metabolism in man and laboratory animals.", "content": "Through the combined use of stable isotope labeling and gas chromatographic mass spectrometric analysis, the metabolic patterns for propoxyphene have been determined in laboratory animals and man. The rat and dog eliminated propoxyphene and its metabolites principally via the bile, while the rabbit more closely resembled man in excreting the metabolic products into urine. Metabolites in rat and rabbit existed as conjugates, whereas in dog and man the metabolites were excreted as a mixture of the free and unconjugated forms. The primary route of metabolism in all species studied was N-demethylation. However, the rat and rabbit extensively hydroxylated propoxyphene and its metabolites prior to elimination. Metabolites arising from ester hydrolysis were found in rat and man. N-acetylated products were identified in all four species. A metabolite formed from cyclization and dehydration of dinorpropoxyphene was isolated in urine and was further identified as a circulating metabolite in dog plasma.", "contents": "Propoxyphene: pathways of metabolism in man and laboratory animals. Through the combined use of stable isotope labeling and gas chromatographic mass spectrometric analysis, the metabolic patterns for propoxyphene have been determined in laboratory animals and man. The rat and dog eliminated propoxyphene and its metabolites principally via the bile, while the rabbit more closely resembled man in excreting the metabolic products into urine. Metabolites in rat and rabbit existed as conjugates, whereas in dog and man the metabolites were excreted as a mixture of the free and unconjugated forms. The primary route of metabolism in all species studied was N-demethylation. However, the rat and rabbit extensively hydroxylated propoxyphene and its metabolites prior to elimination. Metabolites arising from ester hydrolysis were found in rat and man. N-acetylated products were identified in all four species. A metabolite formed from cyclization and dehydration of dinorpropoxyphene was isolated in urine and was further identified as a circulating metabolite in dog plasma.", "PMID": 974236}
{"id": "pubmed23n0032_9808", "title": "Replacement of a chlorine with a methylsulfonyl group in the metabolism of propachlor (2-chloro-N-isopropylacetanilide).", "content": "Urine from rats and sheep given single doses of [14C]propachlor contained 14C metabolites in which the chlorine of propachlor was replaced by a methylsulfonyl group. Methylsulfonyl-containing metabolites were also isolated from the urine of rats given an intraperitoneal dose of the cysteine conjugate of [14C]propachlor; this indicated that the methylsulfonyl-containing metabolites resulted from metabolic reactions subsequent to the mercapturic acid pathway.", "contents": "Replacement of a chlorine with a methylsulfonyl group in the metabolism of propachlor (2-chloro-N-isopropylacetanilide). Urine from rats and sheep given single doses of [14C]propachlor contained 14C metabolites in which the chlorine of propachlor was replaced by a methylsulfonyl group. Methylsulfonyl-containing metabolites were also isolated from the urine of rats given an intraperitoneal dose of the cysteine conjugate of [14C]propachlor; this indicated that the methylsulfonyl-containing metabolites resulted from metabolic reactions subsequent to the mercapturic acid pathway.", "PMID": 974237}
{"id": "pubmed23n0032_9809", "title": "The replacement of trideuteromethyl by methyl groups when using methane as a reagent gas in chemical ionization mass spectrometry.", "content": "The methane chemical ionization spectra of a number of tertiary and quaternary amines with a N-trideuteromethyl group show substitution of this group by methyl. The isotopic purity of the original amines was shown to be greater than 99% using ammonia as a reagent gas.", "contents": "The replacement of trideuteromethyl by methyl groups when using methane as a reagent gas in chemical ionization mass spectrometry. The methane chemical ionization spectra of a number of tertiary and quaternary amines with a N-trideuteromethyl group show substitution of this group by methyl. The isotopic purity of the original amines was shown to be greater than 99% using ammonia as a reagent gas.", "PMID": 974238}
{"id": "pubmed23n0032_9810", "title": "Chemical ionization and field desorption mass spectrometry of the gentamicins.", "content": "Gentamicin sulphate employed in therapeutics consists of a mixture of three major aminoglycoside components, the gentamicins C1, C1a and C2. In the electron impact mass spectra of these components weak parent ions may be observed but they are of no diagnostic value in the commercial mixture. Chemical ionization and field desorption spectra of individual gentamicin components and commercial mixtures are reported and discussed. Isobutane chemical ionization spectra exhibit some fragmentation, but under optimum field desorption conditions little glycosidic cleavage is observed and [M + H]+ ions dominate the spectra.", "contents": "Chemical ionization and field desorption mass spectrometry of the gentamicins. Gentamicin sulphate employed in therapeutics consists of a mixture of three major aminoglycoside components, the gentamicins C1, C1a and C2. In the electron impact mass spectra of these components weak parent ions may be observed but they are of no diagnostic value in the commercial mixture. Chemical ionization and field desorption spectra of individual gentamicin components and commercial mixtures are reported and discussed. Isobutane chemical ionization spectra exhibit some fragmentation, but under optimum field desorption conditions little glycosidic cleavage is observed and [M + H]+ ions dominate the spectra.", "PMID": 974239}
{"id": "pubmed23n0032_9811", "title": "Mass spectrometric investigation of peptide amides.", "content": "The reaction products of trifluoroacetic anhydride with peptide amides are identified as stable heterocyclic imidazolin-5(4)-ones, which can be analysed by gas chromatography mass spectrometry. The mass spectra show intense molecular ions and allow the assignment of the amino acid sequence.", "contents": "Mass spectrometric investigation of peptide amides. The reaction products of trifluoroacetic anhydride with peptide amides are identified as stable heterocyclic imidazolin-5(4)-ones, which can be analysed by gas chromatography mass spectrometry. The mass spectra show intense molecular ions and allow the assignment of the amino acid sequence.", "PMID": 974240}
{"id": "pubmed23n0032_9812", "title": "Field desorption mass spectra of methotrexate and folic acid analogs.", "content": "Field desorption mass spectra of methotrexate and folic acid analogs yield abundant molecular or quasimolecular ions. At emitter currents above the best anode temperature, fragment ions which are useful as further confirmations of structures are observed.", "contents": "Field desorption mass spectra of methotrexate and folic acid analogs. Field desorption mass spectra of methotrexate and folic acid analogs yield abundant molecular or quasimolecular ions. At emitter currents above the best anode temperature, fragment ions which are useful as further confirmations of structures are observed.", "PMID": 974241}
{"id": "pubmed23n0032_9813", "title": "The analysis of terbutaline in plasma and urine at therapeutic levels using mass fragmentography.", "content": "A sensitive method for the qualitative and semiquantitative determination of terbutaline in plasma and urine has been developed. After a single oral dose of 5 mg terbutaline sulphate the drug could be analysed easily. The detection limit in plasma is less than 1 ng ml-1.", "contents": "The analysis of terbutaline in plasma and urine at therapeutic levels using mass fragmentography. A sensitive method for the qualitative and semiquantitative determination of terbutaline in plasma and urine has been developed. After a single oral dose of 5 mg terbutaline sulphate the drug could be analysed easily. The detection limit in plasma is less than 1 ng ml-1.", "PMID": 974242}
{"id": "pubmed23n0032_9814", "title": "Specific deuteromethylation by the Eschweiler-Clarke reaction. Synthesis of differently labelled variants of trimethylamine and their use for the preparation of labelled choline and acetylcholine.", "content": "Reductive methylation (the Eschweiler-Clarke reaction) was used as a route for the synthesis of differently deuterium labelled variants of trimethylamine with the ultimate aim of preparing labelled variants of choline and acetylcholine. Combinations of unlabelled and labelled formaldehyde and formic acid yielded symmetrically labelled trimethylamines of high isotopic purity. The labelled congeners to acetylcholine and choline that were prepared subsequently provide suitable internal standards and tracers to be used in mass spectral analysis and in the evaluation of the pharmacokinetics of the parent compounds.", "contents": "Specific deuteromethylation by the Eschweiler-Clarke reaction. Synthesis of differently labelled variants of trimethylamine and their use for the preparation of labelled choline and acetylcholine. Reductive methylation (the Eschweiler-Clarke reaction) was used as a route for the synthesis of differently deuterium labelled variants of trimethylamine with the ultimate aim of preparing labelled variants of choline and acetylcholine. Combinations of unlabelled and labelled formaldehyde and formic acid yielded symmetrically labelled trimethylamines of high isotopic purity. The labelled congeners to acetylcholine and choline that were prepared subsequently provide suitable internal standards and tracers to be used in mass spectral analysis and in the evaluation of the pharmacokinetics of the parent compounds.", "PMID": 974243}
{"id": "pubmed23n0032_9815", "title": "Hereditary angioedema: modification of clinical manifestations with androgens.", "content": "HAE is an autosomal dominant trait of decreased levels or function of circulating and tissue C'1 esterase inhibitor. The clinical illness is characterized by disabling episodes of peripheral, oropharyngeal and gut-wall edema. Long-term fluoxymesterone treatment of 5 affected males (393 patient-months) and oxymetholone treatment of 6 affected females (204 patient-months) has significantly decreased the frequency of attacks of edema without substantive side effects.", "contents": "Hereditary angioedema: modification of clinical manifestations with androgens. HAE is an autosomal dominant trait of decreased levels or function of circulating and tissue C'1 esterase inhibitor. The clinical illness is characterized by disabling episodes of peripheral, oropharyngeal and gut-wall edema. Long-term fluoxymesterone treatment of 5 affected males (393 patient-months) and oxymetholone treatment of 6 affected females (204 patient-months) has significantly decreased the frequency of attacks of edema without substantive side effects.", "PMID": 974251}
{"id": "pubmed23n0032_9816", "title": "Heterogeneity in the campomelic syndromes: long and short bone varieties.", "content": "There appears to be definite heterogeneity within the campomelic syndromes. We are broadly classifying these into the classic or long bone campomelic syndromes and 2 distinct short bone varieties designated as the normocephalic short-boned and craniosynostotic short-boned types, respectively (Table 1).", "contents": "Heterogeneity in the campomelic syndromes: long and short bone varieties. There appears to be definite heterogeneity within the campomelic syndromes. We are broadly classifying these into the classic or long bone campomelic syndromes and 2 distinct short bone varieties designated as the normocephalic short-boned and craniosynostotic short-boned types, respectively (Table 1).", "PMID": 974255}
{"id": "pubmed23n0032_9817", "title": "Abnormal hemoglobins in a quarter million people.", "content": "Hemolysates of erythrocytes from more than a quarter million people in Alabama were electrophoresed on cellulose acetate, pH 8.4, and those samples exhibiting an abnormality were also electrophoresed in citrate agar, pH 6.0. The globin chains of mutants other than Hb S and C were electrophoresed in urea-mercaptoethanol buffers at both pH 8.9 and pH 6.0, and 60 of them were also analyzed structurally. Of about 6000 samples from whites, only three contained abnormal hemoglobins--Hb D Los Angeles, Hb J Baltimore, and one unidentified. Of 249,000 samples from blacks, about 29,000 contained electrophoretically detectable abnormalities, most of them associated with Hb S or C, present in a frequency of about 9% and 3%, respectively. About 1000 samples resolved into patterns of potential clinical significance. Twenty other mutant hemoglobins were detected, in various genetic combinations in 164 kindreds; four of these-Hb Alabama, Montgomery, Titusville, and Mobile--were previously unknown. The methods used are rapid, economical, and well suited for large scale surveys. They provide highly specific characterizations of many mutant hemoglobins, and no discrepancies were found between the presumptive identifications based on these characterizations and the definitive identifications obtained from structural analyses.", "contents": "Abnormal hemoglobins in a quarter million people. Hemolysates of erythrocytes from more than a quarter million people in Alabama were electrophoresed on cellulose acetate, pH 8.4, and those samples exhibiting an abnormality were also electrophoresed in citrate agar, pH 6.0. The globin chains of mutants other than Hb S and C were electrophoresed in urea-mercaptoethanol buffers at both pH 8.9 and pH 6.0, and 60 of them were also analyzed structurally. Of about 6000 samples from whites, only three contained abnormal hemoglobins--Hb D Los Angeles, Hb J Baltimore, and one unidentified. Of 249,000 samples from blacks, about 29,000 contained electrophoretically detectable abnormalities, most of them associated with Hb S or C, present in a frequency of about 9% and 3%, respectively. About 1000 samples resolved into patterns of potential clinical significance. Twenty other mutant hemoglobins were detected, in various genetic combinations in 164 kindreds; four of these-Hb Alabama, Montgomery, Titusville, and Mobile--were previously unknown. The methods used are rapid, economical, and well suited for large scale surveys. They provide highly specific characterizations of many mutant hemoglobins, and no discrepancies were found between the presumptive identifications based on these characterizations and the definitive identifications obtained from structural analyses.", "PMID": 974261}
{"id": "pubmed23n0032_9818", "title": "Hemoglobin M equon beta 41 (C7) phenylalanine leads to tyrosine.", "content": "A severe hemolytic crisis was observed in a 34-yr-old female of English-Irish extraction following a viral illness treated with acetaminophen. Heinz bodies and heat instability were present only during a transient hemolytic event. A challenge dose of acetaminophen caused no detectable hematologic abnormality. Structural studies of the hemoglobin during hemolysis and again after complete recovery localized the abnormality to tryptic peptide beta Tp-5, and automated sequencing of I 125-labeled beta chains indicated a replacement of phenylalanine (C7) beta 41 by tyrosine. Substitution of the next residue, phenylalanine (CD1) beta 42 by serine (Hb Hammersmith), has resulted in chronic severe Heinz body hemolytic anemia. The lack of chronic anemia in the present disorder may reflect the different relationships of beta41 and beta 42 and/or the similarities in volume and hydrophobicity of tyrosine and phenylalanine. It is suggested that substitution of tyrosine for phenylalanine in Hb Mequon may disturb the critical environment around the heme group and render it susceptible to oxidative denaturation in the presence of infections and/or drugs.", "contents": "Hemoglobin M equon beta 41 (C7) phenylalanine leads to tyrosine. A severe hemolytic crisis was observed in a 34-yr-old female of English-Irish extraction following a viral illness treated with acetaminophen. Heinz bodies and heat instability were present only during a transient hemolytic event. A challenge dose of acetaminophen caused no detectable hematologic abnormality. Structural studies of the hemoglobin during hemolysis and again after complete recovery localized the abnormality to tryptic peptide beta Tp-5, and automated sequencing of I 125-labeled beta chains indicated a replacement of phenylalanine (C7) beta 41 by tyrosine. Substitution of the next residue, phenylalanine (CD1) beta 42 by serine (Hb Hammersmith), has resulted in chronic severe Heinz body hemolytic anemia. The lack of chronic anemia in the present disorder may reflect the different relationships of beta41 and beta 42 and/or the similarities in volume and hydrophobicity of tyrosine and phenylalanine. It is suggested that substitution of tyrosine for phenylalanine in Hb Mequon may disturb the critical environment around the heme group and render it susceptible to oxidative denaturation in the presence of infections and/or drugs.", "PMID": 974262}
{"id": "pubmed23n0032_9819", "title": "Red cell membrane deformability: new data.", "content": "Red cells arrested by fine fibers and subjected to drag forces in a moving stream of fluid can be stressed beyond the point of rupture. The technique, which is basically an in vitro analogue of the microangiopathic hemolytic state, thus permits a study of red cell behavior over the entire range of stress that the membrane can withstand. Measurements made by this approach show an exponential relationship between the velocity of fluid flow and the resultant membrane deformation. Such exponential membrane deformation is consistent with the morphology of red cell fragments and appears to explain their mechanism of formation.", "contents": "Red cell membrane deformability: new data. Red cells arrested by fine fibers and subjected to drag forces in a moving stream of fluid can be stressed beyond the point of rupture. The technique, which is basically an in vitro analogue of the microangiopathic hemolytic state, thus permits a study of red cell behavior over the entire range of stress that the membrane can withstand. Measurements made by this approach show an exponential relationship between the velocity of fluid flow and the resultant membrane deformation. Such exponential membrane deformation is consistent with the morphology of red cell fragments and appears to explain their mechanism of formation.", "PMID": 974263}
{"id": "pubmed23n0032_9820", "title": "Reticulocyte size in nutritional anemias.", "content": "Alterations in reticulocyte size occur 2-3 days after the onset of iron deficient or megaloblastic erythropoiesis and precede, by several weeks, changes in mean corpuscular volume (MCV). Iron-deficiency anemia induced in a normal subject by repeated phlebotomies was characterized by the initial development of larger than normal reticulocytes followed by an abrupt decrease in reticulocyte size. Microreticulocytes appeared 3 days after the fall in per cent iron saturation and antedated the decrease in MCV to below normal by 6 wk. Mean reticulocyte size was disproportionately smaller than normal in patients presenting with iron deficiency. In contrast, reticulocyte size increased abruptly in a patient (and rats) 2-3 days after administration of methotrexate. Mean reticulocyte size was disproportionately larger than normal in patients presenting with folate or vitamin B12 deficiency. Specific replacement therapy with iron, folate, or vitamin B12 was quickly followed by normalization of reticulocyte size.", "contents": "Reticulocyte size in nutritional anemias. Alterations in reticulocyte size occur 2-3 days after the onset of iron deficient or megaloblastic erythropoiesis and precede, by several weeks, changes in mean corpuscular volume (MCV). Iron-deficiency anemia induced in a normal subject by repeated phlebotomies was characterized by the initial development of larger than normal reticulocytes followed by an abrupt decrease in reticulocyte size. Microreticulocytes appeared 3 days after the fall in per cent iron saturation and antedated the decrease in MCV to below normal by 6 wk. Mean reticulocyte size was disproportionately smaller than normal in patients presenting with iron deficiency. In contrast, reticulocyte size increased abruptly in a patient (and rats) 2-3 days after administration of methotrexate. Mean reticulocyte size was disproportionately larger than normal in patients presenting with folate or vitamin B12 deficiency. Specific replacement therapy with iron, folate, or vitamin B12 was quickly followed by normalization of reticulocyte size.", "PMID": 974264}
{"id": "pubmed23n0032_9821", "title": "Specific chromosomal aberrations in polycythemia vera.", "content": "The chromosomes of bone marrow cells from ten patients with polycythemia vera (PV) were identified by Q-, G-, and C-banding techniques. Four of the patients had received no treatment with cytotoxic drugs, while three had received 32P only and the other three, in addition, had received busulfan or busulfan and procarbazine. One 73-yr-old male patient treated with venesection only for 4 yr lacked the Y chromosome and had a deletion of the long arm of chromosome 20 (20q-) in all cells investigated. One of the other three patients who had received no drugs had a chromosome abnormality, but only in 1 of 19 identifiable metaphases. However, the abnormality was the same (+9) as the most common one in treated patients. In the group of treated patients, an extra chromosome 9 (+9) was found in three patients, an extra chromosome 8 (+8) in one, and a deletion of the long arm of one chromosome 20 (20q-) in one patient. Multiple aberrations in addition to the extra chromosome 9 were found in one patient in whom the disease had transformed into acute myeloblastic leukemia. The finding of identical chromosomal aberrations (20q- and +9, respectively) in two patients who had received no drugs and in four patients who had received 32P and busulfan or procarbazine favors the view that these aberrations are specifically associated with the disease and not induced by the drugs. With the exception of the patient with acute myeloblastic leukemia, all other patients are alive 1-11 mo after chromosome analyses and 1-229 mo after diagnosis.", "contents": "Specific chromosomal aberrations in polycythemia vera. The chromosomes of bone marrow cells from ten patients with polycythemia vera (PV) were identified by Q-, G-, and C-banding techniques. Four of the patients had received no treatment with cytotoxic drugs, while three had received 32P only and the other three, in addition, had received busulfan or busulfan and procarbazine. One 73-yr-old male patient treated with venesection only for 4 yr lacked the Y chromosome and had a deletion of the long arm of chromosome 20 (20q-) in all cells investigated. One of the other three patients who had received no drugs had a chromosome abnormality, but only in 1 of 19 identifiable metaphases. However, the abnormality was the same (+9) as the most common one in treated patients. In the group of treated patients, an extra chromosome 9 (+9) was found in three patients, an extra chromosome 8 (+8) in one, and a deletion of the long arm of one chromosome 20 (20q-) in one patient. Multiple aberrations in addition to the extra chromosome 9 were found in one patient in whom the disease had transformed into acute myeloblastic leukemia. The finding of identical chromosomal aberrations (20q- and +9, respectively) in two patients who had received no drugs and in four patients who had received 32P and busulfan or procarbazine favors the view that these aberrations are specifically associated with the disease and not induced by the drugs. With the exception of the patient with acute myeloblastic leukemia, all other patients are alive 1-11 mo after chromosome analyses and 1-229 mo after diagnosis.", "PMID": 974265}
{"id": "pubmed23n0032_9822", "title": "Size distribution, electronic recognition, and counting of human blood monocytes.", "content": "During a study on the separation of human blood monocytes from lymphocytes, a method was developed to recognize and count monocytes by electronic means. Lightscattering (Cytograf, Bio/Physics), and changes in electrical resistance (Channelyzer, Coulter) were used to size mononuclear leukocytes directly in cell suspensions. Both methods revealed a size distribution profile in which two populations of mononuclear leukocytes could be distinguished. The largest cells were virtually eliminated after phagocytosis of iron particles. We confirmed that these cells were monocytes by three different criteria: the intracellular lysozyme activity, the number of phagocytes, and the percentage of cells with kidney-shaped nuclei. The highly significant correlations we found showed that monocytes could be recognized and counted by electronic sizing. For this method, purified mononuclear leukocyte preparations had to be used, since the presence of erythrocytes, platelets, and polymorphonuclear cells interfered. Statistical analysis revealed that electronic sizing permitted discrimination of differences in monocyte content of 4.5%, with a probability of 95%. It was calculated that this sensitivity of electronic monocyte counting was about three times higher than the sensitivity of microscopic methods. Since 100,000 cells can be sized within a few seconds, not only the efficiency of the preparation but also minor changes in the size of monocytes and lymphocytes introduced during the isolation can be followed.", "contents": "Size distribution, electronic recognition, and counting of human blood monocytes. During a study on the separation of human blood monocytes from lymphocytes, a method was developed to recognize and count monocytes by electronic means. Lightscattering (Cytograf, Bio/Physics), and changes in electrical resistance (Channelyzer, Coulter) were used to size mononuclear leukocytes directly in cell suspensions. Both methods revealed a size distribution profile in which two populations of mononuclear leukocytes could be distinguished. The largest cells were virtually eliminated after phagocytosis of iron particles. We confirmed that these cells were monocytes by three different criteria: the intracellular lysozyme activity, the number of phagocytes, and the percentage of cells with kidney-shaped nuclei. The highly significant correlations we found showed that monocytes could be recognized and counted by electronic sizing. For this method, purified mononuclear leukocyte preparations had to be used, since the presence of erythrocytes, platelets, and polymorphonuclear cells interfered. Statistical analysis revealed that electronic sizing permitted discrimination of differences in monocyte content of 4.5%, with a probability of 95%. It was calculated that this sensitivity of electronic monocyte counting was about three times higher than the sensitivity of microscopic methods. Since 100,000 cells can be sized within a few seconds, not only the efficiency of the preparation but also minor changes in the size of monocytes and lymphocytes introduced during the isolation can be followed.", "PMID": 974266}
{"id": "pubmed23n0032_9823", "title": "Surface markers of human eosinophils.", "content": "Peripheral blood eosinophils from patients with eosinophilia and from healthy subjects were studied for surface immunoglobulins, receptors for the Fc region of IgG, complement receptors, and spontaneous rosette formation with sheep and mouse erythrocytes. Eosinophils were found to have receptors for complement and for aggregated IgG, and to have the same two types of complement receptors as do lymphocytes and monocytes. Immune adherence type receptors were specific for C4 or C3b, while C3d receptors were specific for C3d but unreactive with C4. Eosinophils differed from fully mature neutrophils in that the former had C3d receptors and relatively weak immune adherence (C4 or C3b) receptors, while the later did not have the C3d receptors and had strong immune adherence receptors. Eosinophil phagocytosis of complement-receptor bound erythrocytes was dependent on the presence of IgG in the antibody coating the red blood cells; this requirement for IgG resembled that found in neutrophil phagocytosis. No surface Ig or spontaneous erythrocyte rosette formation was observed with eosinophils.", "contents": "Surface markers of human eosinophils. Peripheral blood eosinophils from patients with eosinophilia and from healthy subjects were studied for surface immunoglobulins, receptors for the Fc region of IgG, complement receptors, and spontaneous rosette formation with sheep and mouse erythrocytes. Eosinophils were found to have receptors for complement and for aggregated IgG, and to have the same two types of complement receptors as do lymphocytes and monocytes. Immune adherence type receptors were specific for C4 or C3b, while C3d receptors were specific for C3d but unreactive with C4. Eosinophils differed from fully mature neutrophils in that the former had C3d receptors and relatively weak immune adherence (C4 or C3b) receptors, while the later did not have the C3d receptors and had strong immune adherence receptors. Eosinophil phagocytosis of complement-receptor bound erythrocytes was dependent on the presence of IgG in the antibody coating the red blood cells; this requirement for IgG resembled that found in neutrophil phagocytosis. No surface Ig or spontaneous erythrocyte rosette formation was observed with eosinophils.", "PMID": 974267}
{"id": "pubmed23n0032_9824", "title": "Stimulation of megakaryocytopoiesis by acute thrombocytopenia in rats.", "content": "Rats were made acutely thrombocytopenic by injection of antiplatelet serum. Marrow sections and squash preparations were made at intervals during 120 hr. Determinations were made of mitotic index, stage of maturation, ploidy level, and cell size of megakaryocytes; number and size of platelets were measured. Increased endomitosis among megakaryocytes was followed by an increase in the proportion of immature megakaryocytes, a greater average ploidy level of recognized megakaryocytes, and larger megakaryocytes. Maximum changes in these several parameters occurred between 32 and 72 hr after induction of thrombocytopenia. By 120 hr all megakarocyte parameters were near normal. For about 3 days, beginning at about 36 hr, platelet numbers increased rapidly. Average platelet size rose and returned to normal within about 60 hr. Changes in ploidy and size of megakaryocytes were measured in the immature and mature maturation stages. The results suggest that the initial stimulus in response to acute thrombocytopenia acts primarily on diploid precursors, programming them to mature into a population of megakaryocytes with an average ploidy approximately one level greater than in normal rats and a proportionate increase in cell size. The larger megakaryocytes presumably produce more platelets, accounting for a major part of the increased rate of platelet production. Since the changes in megakaryocytes begin to reverse before circulating platelet numbers have reached the normal level, reversal of the stimulus appears to be initiated by some change other than platelet mass.", "contents": "Stimulation of megakaryocytopoiesis by acute thrombocytopenia in rats. Rats were made acutely thrombocytopenic by injection of antiplatelet serum. Marrow sections and squash preparations were made at intervals during 120 hr. Determinations were made of mitotic index, stage of maturation, ploidy level, and cell size of megakaryocytes; number and size of platelets were measured. Increased endomitosis among megakaryocytes was followed by an increase in the proportion of immature megakaryocytes, a greater average ploidy level of recognized megakaryocytes, and larger megakaryocytes. Maximum changes in these several parameters occurred between 32 and 72 hr after induction of thrombocytopenia. By 120 hr all megakarocyte parameters were near normal. For about 3 days, beginning at about 36 hr, platelet numbers increased rapidly. Average platelet size rose and returned to normal within about 60 hr. Changes in ploidy and size of megakaryocytes were measured in the immature and mature maturation stages. The results suggest that the initial stimulus in response to acute thrombocytopenia acts primarily on diploid precursors, programming them to mature into a population of megakaryocytes with an average ploidy approximately one level greater than in normal rats and a proportionate increase in cell size. The larger megakaryocytes presumably produce more platelets, accounting for a major part of the increased rate of platelet production. Since the changes in megakaryocytes begin to reverse before circulating platelet numbers have reached the normal level, reversal of the stimulus appears to be initiated by some change other than platelet mass.", "PMID": 974268}
{"id": "pubmed23n0032_9825", "title": "Collagen metabolism during the early stages of cholesterol-induced atherogenesis in rabbits.", "content": "The involvement of collagen in cholesterol-induced atherosclerosis in rabbits was investigated. Rabbits were fed a 2% cholesterol diet for 8, 16, 30, 60 and 90 days. Histological sections were taken and aortic free and esterified cholesterol were determined after separation on thin-layer chromatography. Prolyl hydroxylase activity was used as a measure of collagen synthetic rate and hydroxyproline levels as an estimate of collagen content. Cholesterol content was a significantly increased after 8 days, while at this time there were no gross aortic lesions. After 30 days there was some aortic disease and by 60 days most of the rabbits exhibited pronounced aortic lesions. Histologically, the lesions consisted mainly of intimal foam cells. There was no alteration in collagen synthetic rate or content at 8, 16, 30 or 60 days. These data indicate that 60 days of continuous cholesterol feeding results in a foam cell aortic lesion with no alteration in collagen metabolism. After 90 days of cholesterol feeding there was significant increase in collagen synthetic activity in the thoracic aorta. These data suggest that alteration of collagen synthetic activity is secondary response, resulting from injury induced by the aortic accumulation of large amounts of cholesterol.", "contents": "Collagen metabolism during the early stages of cholesterol-induced atherogenesis in rabbits. The involvement of collagen in cholesterol-induced atherosclerosis in rabbits was investigated. Rabbits were fed a 2% cholesterol diet for 8, 16, 30, 60 and 90 days. Histological sections were taken and aortic free and esterified cholesterol were determined after separation on thin-layer chromatography. Prolyl hydroxylase activity was used as a measure of collagen synthetic rate and hydroxyproline levels as an estimate of collagen content. Cholesterol content was a significantly increased after 8 days, while at this time there were no gross aortic lesions. After 30 days there was some aortic disease and by 60 days most of the rabbits exhibited pronounced aortic lesions. Histologically, the lesions consisted mainly of intimal foam cells. There was no alteration in collagen synthetic rate or content at 8, 16, 30 or 60 days. These data indicate that 60 days of continuous cholesterol feeding results in a foam cell aortic lesion with no alteration in collagen metabolism. After 90 days of cholesterol feeding there was significant increase in collagen synthetic activity in the thoracic aorta. These data suggest that alteration of collagen synthetic activity is secondary response, resulting from injury induced by the aortic accumulation of large amounts of cholesterol.", "PMID": 974270}
{"id": "pubmed23n0032_9826", "title": "A comparative study of the role played by some inactivation pathways in the disposition of the transmitter in the rabbit aorta and the saphenous vein of the dog.", "content": "The aim of this study was to determine the relative importance of neuronal uptake, catechol-O-methyltransferase (COMT) and diffusion in the disposition of endogenous nor-adrenaline (NA) in the rabbit aorta and dog saphenous vein. Helical strips of dog saphenous vein and rabbit aorta were stimulated transmurally, relaxation occurring either in oil or in Krebs-Henseleit solution. The rate of inactivation of NA by various mechanisms was assessed from the decrease in the rate of relaxation after blockade of one or more of these processes. Cocaine was used to inhibit neuronal uptake and COMT was blocked with 3,4-dihydroxy-2-methyl propiophenone. Diffusion out of the tissue was prevented by replacing the Krebs-Henseleit solution in the muscle bath with mineral oil. Neuronal uptake was the primary mechanism of inactivation in the saphenous vein whereas diffusion and COMT appear to be more important in the aorta. The differences in the relative importance of the inactivation mechanisms (neuronal uptake, O-methyltransferase and diffusion) existing between these two vessels are attributed primarily to differing density and distribution of the adrenergic innervation.", "contents": "A comparative study of the role played by some inactivation pathways in the disposition of the transmitter in the rabbit aorta and the saphenous vein of the dog. The aim of this study was to determine the relative importance of neuronal uptake, catechol-O-methyltransferase (COMT) and diffusion in the disposition of endogenous nor-adrenaline (NA) in the rabbit aorta and dog saphenous vein. Helical strips of dog saphenous vein and rabbit aorta were stimulated transmurally, relaxation occurring either in oil or in Krebs-Henseleit solution. The rate of inactivation of NA by various mechanisms was assessed from the decrease in the rate of relaxation after blockade of one or more of these processes. Cocaine was used to inhibit neuronal uptake and COMT was blocked with 3,4-dihydroxy-2-methyl propiophenone. Diffusion out of the tissue was prevented by replacing the Krebs-Henseleit solution in the muscle bath with mineral oil. Neuronal uptake was the primary mechanism of inactivation in the saphenous vein whereas diffusion and COMT appear to be more important in the aorta. The differences in the relative importance of the inactivation mechanisms (neuronal uptake, O-methyltransferase and diffusion) existing between these two vessels are attributed primarily to differing density and distribution of the adrenergic innervation.", "PMID": 974271}
{"id": "pubmed23n0032_9827", "title": "Phospho-accepting proteins in bovine sera.", "content": "Phospho-accepting proteins in bovine sera have been detected by the use of immobilized protein kinase from rat muscle and (gamma32P)-ATP in an in vitro system. A partial biochemical characterization points to the generation of typical phosphoproteins. Differences in the phosphorylation pattern between fetal serum and calf serum as demonstrated by electrophoresis in the presence of dodecylsulfate are described.", "contents": "Phospho-accepting proteins in bovine sera. Phospho-accepting proteins in bovine sera have been detected by the use of immobilized protein kinase from rat muscle and (gamma32P)-ATP in an in vitro system. A partial biochemical characterization points to the generation of typical phosphoproteins. Differences in the phosphorylation pattern between fetal serum and calf serum as demonstrated by electrophoresis in the presence of dodecylsulfate are described.", "PMID": 974272}
{"id": "pubmed23n0032_9828", "title": "Decrease of normotest/thrombotest discrepancy in non-contacted coumarin plasma after glass or ellagic acid activation.", "content": "Activation of coumarin plasmas in glass tubes for 60 min resulted in a clear shortening of Thrombotest clotting times. Normotest clotting times were shortened too but to a much lesser extent. As a consequence the Normotest (NT)/Thrombotest (TT) discrepancy which was quite large at 0 time, became progressively smaller. This phenomenon was observed both in undiluted and 6:10 diluted plasma. After ellagic acid activation a similar phenomenon was noted even though a less pronounced shortening of Thrombotest was noted. These data suggest that Thrombotest is very sensitive to contact phase and to factor VII activation and not to any coumarin-induced inhibitors.", "contents": "Decrease of normotest/thrombotest discrepancy in non-contacted coumarin plasma after glass or ellagic acid activation. Activation of coumarin plasmas in glass tubes for 60 min resulted in a clear shortening of Thrombotest clotting times. Normotest clotting times were shortened too but to a much lesser extent. As a consequence the Normotest (NT)/Thrombotest (TT) discrepancy which was quite large at 0 time, became progressively smaller. This phenomenon was observed both in undiluted and 6:10 diluted plasma. After ellagic acid activation a similar phenomenon was noted even though a less pronounced shortening of Thrombotest was noted. These data suggest that Thrombotest is very sensitive to contact phase and to factor VII activation and not to any coumarin-induced inhibitors.", "PMID": 974273}
{"id": "pubmed23n0032_9829", "title": "Lead in tissues of mallard ducks dosed with two types of lead shot.", "content": "Mallard ducks (Anas platyrhynchos) were sacrificed one month after ingesting one number 4 all-lead shot or one number 4 lead-iron shot. Livers, kidneys, blood, wingbones, and eggs were analyzed for lead by atomic absorption. Necropsy of sacrificed ducks failed to reveal any of the tissue lesions usually associated with lead poisoning in waterfowl. Lead levels in ducks given all-lead shot averaged about twice those in ducks given lead-iron shot, reflecting the amount of lead in the two types of shot. Lead in the blood of ducks dosed with all-lead shot averaged 0.64 ppm, and 0.28 ppm in ducks given lead-iron shot. Lead residues in livers and kidneys of females given all-lead shot were significantly higher than in males. In both dosed groups, lead levels in wingbones of females were about 10 times those in males, and were significantly correlated with the number of eggs laid after dosage. Lead levels in contents and shells of eggs laid by hens dosed with all-lead shot were about twice those in eggs laid by hens dosed with lead-iron shot. Eggshells were found to best reflect levels of lead in the blood. Our results indicate that mallards maintained on a balanced diet and dosed with one lead shot may not accumulate extremely high lead levels in the liver and kidney. However, extremely high lead deposition may result in the bone of laying hens after ingesting sublethal amounts of lead shot as a result of mobilization of calcium from the bone during eggshell formation.", "contents": "Lead in tissues of mallard ducks dosed with two types of lead shot. Mallard ducks (Anas platyrhynchos) were sacrificed one month after ingesting one number 4 all-lead shot or one number 4 lead-iron shot. Livers, kidneys, blood, wingbones, and eggs were analyzed for lead by atomic absorption. Necropsy of sacrificed ducks failed to reveal any of the tissue lesions usually associated with lead poisoning in waterfowl. Lead levels in ducks given all-lead shot averaged about twice those in ducks given lead-iron shot, reflecting the amount of lead in the two types of shot. Lead in the blood of ducks dosed with all-lead shot averaged 0.64 ppm, and 0.28 ppm in ducks given lead-iron shot. Lead residues in livers and kidneys of females given all-lead shot were significantly higher than in males. In both dosed groups, lead levels in wingbones of females were about 10 times those in males, and were significantly correlated with the number of eggs laid after dosage. Lead levels in contents and shells of eggs laid by hens dosed with all-lead shot were about twice those in eggs laid by hens dosed with lead-iron shot. Eggshells were found to best reflect levels of lead in the blood. Our results indicate that mallards maintained on a balanced diet and dosed with one lead shot may not accumulate extremely high lead levels in the liver and kidney. However, extremely high lead deposition may result in the bone of laying hens after ingesting sublethal amounts of lead shot as a result of mobilization of calcium from the bone during eggshell formation.", "PMID": 974275}
{"id": "pubmed23n0032_9830", "title": "Plasticizers as interferences in pollutant analyses.", "content": "Airborne compounds from two groups of plasticizers used in air conditioner filters and caulks as well as flexible polyvinyl chloride and related plastics have been identified as major sources of low level laboratory contamination that can cause serious interferences in pollutant analyses especially at the low levels requiring GCEC for quantitation. Two compounds from the phthalic acid ester group (PAE'S) OF PLASTICIZERS THAT ARE ENVIRONMENTAL POLLUTANTS AS WELL WERE FOUND INCORPORATED ON AIR CONDITIONER FILTERS LEADING TO CONTAMINATION OF CLEAN AND OIL FILMED DISHES FROM AIR FALLOUT. Solvents, inorganic reagents and hand-to-glass transfer were found to contribute to sample contamination by PAE's. Several PAE's are easily detectable at 0.1 ng levels under some conditions on GCEC and have retention times on several columns of differing polarities that can interfere with analyses of chlorinated pollutants especially p,p'-DDT. Aroclor 1254, a member of the polychlorinated biphenyl group (PCB's) of plasticizers, was found in a caulk around a laboratory window. On oil filmed, but not clean dishes, the compounds collected from air fallout produced chromatograms on GCEC with a close correspondence to Aroclor 1242.", "contents": "Plasticizers as interferences in pollutant analyses. Airborne compounds from two groups of plasticizers used in air conditioner filters and caulks as well as flexible polyvinyl chloride and related plastics have been identified as major sources of low level laboratory contamination that can cause serious interferences in pollutant analyses especially at the low levels requiring GCEC for quantitation. Two compounds from the phthalic acid ester group (PAE'S) OF PLASTICIZERS THAT ARE ENVIRONMENTAL POLLUTANTS AS WELL WERE FOUND INCORPORATED ON AIR CONDITIONER FILTERS LEADING TO CONTAMINATION OF CLEAN AND OIL FILMED DISHES FROM AIR FALLOUT. Solvents, inorganic reagents and hand-to-glass transfer were found to contribute to sample contamination by PAE's. Several PAE's are easily detectable at 0.1 ng levels under some conditions on GCEC and have retention times on several columns of differing polarities that can interfere with analyses of chlorinated pollutants especially p,p'-DDT. Aroclor 1254, a member of the polychlorinated biphenyl group (PCB's) of plasticizers, was found in a caulk around a laboratory window. On oil filmed, but not clean dishes, the compounds collected from air fallout produced chromatograms on GCEC with a close correspondence to Aroclor 1242.", "PMID": 974279}
{"id": "pubmed23n0032_9831", "title": "Some aspects of orthopaedic surgery in childhood.", "content": "The aetiological role of immunodeficiency in acute septic arthritis of the hip in infancy, the management of the condition after the acute infection has subsided, and the special hazards of infection in the region of the hip joint in the older child are discussed. The principles of treatment of congenital dislocation of the hip are examined in relation to the maintenance of acetabular growth potential. The factors determining the outcome of treatment in Perthes' disease are discussed and a comparison of the result in a series of cases treated by femoral osteotomy with those in untreated controls is presented.", "contents": "Some aspects of orthopaedic surgery in childhood. The aetiological role of immunodeficiency in acute septic arthritis of the hip in infancy, the management of the condition after the acute infection has subsided, and the special hazards of infection in the region of the hip joint in the older child are discussed. The principles of treatment of congenital dislocation of the hip are examined in relation to the maintenance of acetabular growth potential. The factors determining the outcome of treatment in Perthes' disease are discussed and a comparison of the result in a series of cases treated by femoral osteotomy with those in untreated controls is presented.", "PMID": 974284}
{"id": "pubmed23n0032_9832", "title": "Steroid induced avascular necrosis of bones in eighteen sites: a case report.", "content": "A patient with idiopathic thrombocytopenia purpura who was treated with high dosage corticosteroids for ten years, developed 18 separate lesions of avascular necrosis in 12 different bones. Despite spontaneous revascularization of many of the lesions, subchrondral collapse caused symptomatic secondary degenerative arthritis in many joints. Pathological examination of both resected femoral heads showed no intravascular thrombosis.", "contents": "Steroid induced avascular necrosis of bones in eighteen sites: a case report. A patient with idiopathic thrombocytopenia purpura who was treated with high dosage corticosteroids for ten years, developed 18 separate lesions of avascular necrosis in 12 different bones. Despite spontaneous revascularization of many of the lesions, subchrondral collapse caused symptomatic secondary degenerative arthritis in many joints. Pathological examination of both resected femoral heads showed no intravascular thrombosis.", "PMID": 974285}
{"id": "pubmed23n0032_9833", "title": "Irreducible fractures of the distal phalanx of the hand.", "content": "Fractures of the distal phalanx of the hand occur usually by crushing can be irreducible, if the nail fold is interposed between the fracture fragments. Details of the regional anatomy producing the deformity and the management are discussed.", "contents": "Irreducible fractures of the distal phalanx of the hand. Fractures of the distal phalanx of the hand occur usually by crushing can be irreducible, if the nail fold is interposed between the fracture fragments. Details of the regional anatomy producing the deformity and the management are discussed.", "PMID": 974286}
{"id": "pubmed23n0032_9834", "title": "Flexor digitorum superficialis rupture: a case report.", "content": "A rupture of the musculotendinous junction of the flexor digitorum superficialis to the index finger in a baseball pitcher is described. No underlying abnormality could be detected. Because of loss of strength and dexterity, repair of the rupture was accomplished with an improved result.", "contents": "Flexor digitorum superficialis rupture: a case report. A rupture of the musculotendinous junction of the flexor digitorum superficialis to the index finger in a baseball pitcher is described. No underlying abnormality could be detected. Because of loss of strength and dexterity, repair of the rupture was accomplished with an improved result.", "PMID": 974287}
{"id": "pubmed23n0032_9835", "title": "Studies of the corrosion susceptibility of metallic cement restrictors: comparative corrosion behavior of stainless steel and cobalt-chromium alloys.", "content": "Passive film and accelerated corrosion tests were performed on both wrought AISI 316L stainless steel and wrought Vitallium (Haynes Stellite 25) Charnley Type cement restrictors. The morphology and incidence of corrosion at the wire-wire crevices and the methacrylate-metal crevices were determined by scanning electron microscopy. The results are interpreted in view of the previously-documented crevice corrosion susceptibility of these two alloys.", "contents": "Studies of the corrosion susceptibility of metallic cement restrictors: comparative corrosion behavior of stainless steel and cobalt-chromium alloys. Passive film and accelerated corrosion tests were performed on both wrought AISI 316L stainless steel and wrought Vitallium (Haynes Stellite 25) Charnley Type cement restrictors. The morphology and incidence of corrosion at the wire-wire crevices and the methacrylate-metal crevices were determined by scanning electron microscopy. The results are interpreted in view of the previously-documented crevice corrosion susceptibility of these two alloys.", "PMID": 974288}
{"id": "pubmed23n0032_9836", "title": "Pain and the pronator teres syndrome.", "content": "Extremely painful paresthesias and mild motor weakness of the hand were relieved by sectioning the deep head of the pronator teres muscle and the fibrous arch of the flexor digitorum superficialis, thereby freeing the median nerve and its anterior interosseous branch. The characteristic pinch deformity of the thumb and index fingers usually described in anterior interosseous-nerve syndrome was not present. A Tinel sign at the level of the pronator teres was present.", "contents": "Pain and the pronator teres syndrome. Extremely painful paresthesias and mild motor weakness of the hand were relieved by sectioning the deep head of the pronator teres muscle and the fibrous arch of the flexor digitorum superficialis, thereby freeing the median nerve and its anterior interosseous branch. The characteristic pinch deformity of the thumb and index fingers usually described in anterior interosseous-nerve syndrome was not present. A Tinel sign at the level of the pronator teres was present.", "PMID": 974290}
{"id": "pubmed23n0032_9837", "title": "On the uses of diversity.", "content": "An examination is made of the uses of diversity in medical library activities and personnel. This concept is extended also to the broader field of librarianship and to some forecasts of future directions for medical libraries.", "contents": "On the uses of diversity. An examination is made of the uses of diversity in medical library activities and personnel. This concept is extended also to the broader field of librarianship and to some forecasts of future directions for medical libraries.", "PMID": 974292}
{"id": "pubmed23n0032_9838", "title": "Planning for meaningful change in libraries and library networks: a first step.", "content": "The Program Planning Model (PPM) is a planning and management tool that facilitates development and acceptance of significant programs. PPM assists program planning by systematically involving consumer or user groups in the development and review of new programs. While user participitation is important throughout PPM, this involvement is especially important during the problem exploration phase, when the problems or needs of these users are surveyed. The Nominal Group Technique (NGT), a variation of the \"brainstorming\" process, is a structured but flexible group process used in the problem exploration phase to generate a high number of useful responses concerning problem areas. NGT ensures maximum contribution of ideas by all participtants. NGT and its application in the planning and management efforts of several libraries and library networks are described in detail.", "contents": "Planning for meaningful change in libraries and library networks: a first step. The Program Planning Model (PPM) is a planning and management tool that facilitates development and acceptance of significant programs. PPM assists program planning by systematically involving consumer or user groups in the development and review of new programs. While user participitation is important throughout PPM, this involvement is especially important during the problem exploration phase, when the problems or needs of these users are surveyed. The Nominal Group Technique (NGT), a variation of the \"brainstorming\" process, is a structured but flexible group process used in the problem exploration phase to generate a high number of useful responses concerning problem areas. NGT ensures maximum contribution of ideas by all participtants. NGT and its application in the planning and management efforts of several libraries and library networks are described in detail.", "PMID": 974293}
{"id": "pubmed23n0032_9839", "title": "Nonmediated use of MEDLINE and TOXLINE by pathologists and pharmacists.", "content": "Pathologists and pharmacists have used terminals in their departmental areas successfully to perform searches on MEDLINE and TOXLINE, during the period from fall 1974 through August 1975; MEDLINE has been used more than TOXLINE. In the Department of Pathology at the University of Maryland Health Sciences Center, the annual rate of combined mediated and nonmediated use of MEDLINE increased from 142 search sessions to 432, over half of the identified use being by three individuals. About half of the total potential users from each group tried MEDLINE or TOXLINE at least once. Nine pathologists and seven pharmacists used MEDLINE five or more times. Five of the individuals in each group were \"intensive\" users, who concentrated more than half of their sessions within two months. Vocabulary experience and systems problems are touched on. Most individuals learned to use the systems from one another or in individual sessions with the authors, although a twenty-five page \"minimanual\" has also been used by a limited group.", "contents": "Nonmediated use of MEDLINE and TOXLINE by pathologists and pharmacists. Pathologists and pharmacists have used terminals in their departmental areas successfully to perform searches on MEDLINE and TOXLINE, during the period from fall 1974 through August 1975; MEDLINE has been used more than TOXLINE. In the Department of Pathology at the University of Maryland Health Sciences Center, the annual rate of combined mediated and nonmediated use of MEDLINE increased from 142 search sessions to 432, over half of the identified use being by three individuals. About half of the total potential users from each group tried MEDLINE or TOXLINE at least once. Nine pathologists and seven pharmacists used MEDLINE five or more times. Five of the individuals in each group were \"intensive\" users, who concentrated more than half of their sessions within two months. Vocabulary experience and systems problems are touched on. Most individuals learned to use the systems from one another or in individual sessions with the authors, although a twenty-five page \"minimanual\" has also been used by a limited group.", "PMID": 974294}
{"id": "pubmed23n0032_9840", "title": "Primary journal selection using citations from an indexing service journal: a method and example from nursing literature.", "content": "Although serial literature is extremely important to a library collection, it is also the source of many problems. Specialty journal selection is difficult, particularly for the librarian of a small or intermediate-size library that is not in a position to develop or maintain an exhaustive or inclusive collection in a particular field or discipline. Steadily increasing journal costs and recent economic trends necessitate establishment or reexamination of a periodical collection policy. In this investigation, the technique used analyzes citations assigned to medical subject headings (MeSH) and subheadings by indexers who prepare the MEDLARS data base. Citations have been retrieved by exploiting the on-line nature of the MEDLARS data base. A four-year time period is used to identify specialty journals in the area of nursing. Results given include a separate rank-order listing arranged by decreasing frequency of productivity for each MeSH term searched. A composite listing is given for the 16,355 unique citations retrieved. The approach illustrated and data presented may be useful in establishing library policy for questions of periodical subscription and setting of priorities for binding and microform purchases. The purpose of the approach described is to predict collection demand with efficiency and economy.", "contents": "Primary journal selection using citations from an indexing service journal: a method and example from nursing literature. Although serial literature is extremely important to a library collection, it is also the source of many problems. Specialty journal selection is difficult, particularly for the librarian of a small or intermediate-size library that is not in a position to develop or maintain an exhaustive or inclusive collection in a particular field or discipline. Steadily increasing journal costs and recent economic trends necessitate establishment or reexamination of a periodical collection policy. In this investigation, the technique used analyzes citations assigned to medical subject headings (MeSH) and subheadings by indexers who prepare the MEDLARS data base. Citations have been retrieved by exploiting the on-line nature of the MEDLARS data base. A four-year time period is used to identify specialty journals in the area of nursing. Results given include a separate rank-order listing arranged by decreasing frequency of productivity for each MeSH term searched. A composite listing is given for the 16,355 unique citations retrieved. The approach illustrated and data presented may be useful in establishing library policy for questions of periodical subscription and setting of priorities for binding and microform purchases. The purpose of the approach described is to predict collection demand with efficiency and economy.", "PMID": 974295}
{"id": "pubmed23n0032_9841", "title": "New library buildings. Part VIII. The Library of the Health Sciences, University of Illinois at the Medical Center, Chicago.", "content": "The new Library of the Health Sciences at the University of Illinois at the Medical Center is described and illustrated. The building was under construction for thirteen months, using phased construction techniques, and opened in October 1973. The library is a self-contained and physically independent building consisting of three levels above ground and one lower level, comprising an area of 130,000 sq. ft. gross and 88,000 sq. ft. net. The building provides space for 350,000 volumes, 1,037 readers, and a staff of 100. The cost of construction was $5,140,500 plus $858,500 for furnishings and equipment. Among the special features are a twenty-four-hour multimedia services department and a full-circulating style book detection system. Included in the article is an evaluation of the library after two years of occupancy.", "contents": "New library buildings. Part VIII. The Library of the Health Sciences, University of Illinois at the Medical Center, Chicago. The new Library of the Health Sciences at the University of Illinois at the Medical Center is described and illustrated. The building was under construction for thirteen months, using phased construction techniques, and opened in October 1973. The library is a self-contained and physically independent building consisting of three levels above ground and one lower level, comprising an area of 130,000 sq. ft. gross and 88,000 sq. ft. net. The building provides space for 350,000 volumes, 1,037 readers, and a staff of 100. The cost of construction was $5,140,500 plus $858,500 for furnishings and equipment. Among the special features are a twenty-four-hour multimedia services department and a full-circulating style book detection system. Included in the article is an evaluation of the library after two years of occupancy.", "PMID": 974296}
{"id": "pubmed23n0032_9842", "title": "Leptospirosis as an animal and public health problem in Latin America and the Caribbean area.", "content": "As a major livestock disease, leptospirosis cuts significantly into the world's supply of animal protein through its consequences of abortion and calf mortality, retarded growth, and reduced milk production. Evidently, such losses have severe economic implications as well. The disease's impact has been somewhat diminished in the developed countries thanks to mechanization of rural activities, improved personal and environmental hygiene, and broad application of preventive measures, including the administration of bacterins, in domestic animals. Still, even in these circumstances it remains a serious problem. In the developing countries, in which large percentages of the population live in close contact with animals, the spread of infection is much greater. Moreover, the ecology of tropical and subtropical America--with its abundant rainfall, natural water courses, and high temperatures--is particularly favorable for the transmission of leptospires. In such conditions the human disease is a growing threat as well. The spread of leptospirosis, both in animals and man, is facilitated by the fact that inefection from many of the serotypes can exist without presenting serious clinical manifestations. Hence the importance of laboratory diagnosis. Systematic studies over time of serotype distribution, coupled with wide and improved surveillance, will be a key factor in bringing the disease under control. According to the limited seroepidemiologic data available, 3 to 18 per cent of the general human population, 2 to 32 per cent of those belonging to exposed occupational groups, and 10 to 50 per cent of patients with fever of unknown origin have leptospiral antibody. The most predominant serogroups are Icterohaemorrhagiae, Canicola, Pomona, Hebdomadis, and Bataviae. So far more than 50 serotypes, from 15 serogroups, have been isolated from man and animals in Latin America and the Caribbean area.", "contents": "Leptospirosis as an animal and public health problem in Latin America and the Caribbean area. As a major livestock disease, leptospirosis cuts significantly into the world's supply of animal protein through its consequences of abortion and calf mortality, retarded growth, and reduced milk production. Evidently, such losses have severe economic implications as well. The disease's impact has been somewhat diminished in the developed countries thanks to mechanization of rural activities, improved personal and environmental hygiene, and broad application of preventive measures, including the administration of bacterins, in domestic animals. Still, even in these circumstances it remains a serious problem. In the developing countries, in which large percentages of the population live in close contact with animals, the spread of infection is much greater. Moreover, the ecology of tropical and subtropical America--with its abundant rainfall, natural water courses, and high temperatures--is particularly favorable for the transmission of leptospires. In such conditions the human disease is a growing threat as well. The spread of leptospirosis, both in animals and man, is facilitated by the fact that inefection from many of the serotypes can exist without presenting serious clinical manifestations. Hence the importance of laboratory diagnosis. Systematic studies over time of serotype distribution, coupled with wide and improved surveillance, will be a key factor in bringing the disease under control. According to the limited seroepidemiologic data available, 3 to 18 per cent of the general human population, 2 to 32 per cent of those belonging to exposed occupational groups, and 10 to 50 per cent of patients with fever of unknown origin have leptospiral antibody. The most predominant serogroups are Icterohaemorrhagiae, Canicola, Pomona, Hebdomadis, and Bataviae. So far more than 50 serotypes, from 15 serogroups, have been isolated from man and animals in Latin America and the Caribbean area.", "PMID": 974301}
{"id": "pubmed23n0032_9843", "title": "Immunization against leptospirosis: vaccine trials with heat-killed whole cell and outer envelope antigens in hamsters.", "content": "Heat-killed whole cell and outer envelope antigens prepared from homologous virulent and avirulent strains of Leptospira serotypes canicola and pomona were evaluated for protecting hamsters against experimental leptospirosis. The heat-killed bacterins proved at least as effective as the outer envelope antigens, or more so, in providing protection against death and infection, and they are easier and more economical to prepare.", "contents": "Immunization against leptospirosis: vaccine trials with heat-killed whole cell and outer envelope antigens in hamsters. Heat-killed whole cell and outer envelope antigens prepared from homologous virulent and avirulent strains of Leptospira serotypes canicola and pomona were evaluated for protecting hamsters against experimental leptospirosis. The heat-killed bacterins proved at least as effective as the outer envelope antigens, or more so, in providing protection against death and infection, and they are easier and more economical to prepare.", "PMID": 974302}
{"id": "pubmed23n0032_9844", "title": "Diarrheal diseases of infancy in Cali, Colombia: study design and summary report on isolated disease agents.", "content": "For public health reasons, it is important that the etiologic agents of early childhood diarrhea be isolated and identified, and that their routes of transmission be defined. This is especially true in tropical and subtropical developing countries, where childhood patterns of exposure to diarrheal disease agents usually differ from those in developed countries, and where diarrheal illness is a frequent harbinger of death among children under five years of age. This artical describes a study designed to identify diarrheal disease agents and transmission patterns in Cali, a large city of western Colombia's fertile Cauca River Valley. The study area, composed of five working-class districts with a total population of some 40,000, appeared to provide an environment fairly similar to those of many other \"average\" working-class communities in Latin America. Beginning in July 1962, a cohort of 296 children being born in these districts was studied, the period of investigation starting with the date of birth and continuing until each child's second birthday or its premature withdrawal from the study. Weekly home visits were made to establish defecation patterns, feeding practices, and anthropometry. The resulting data were then analyzed in terms of defecation frequencies, occurrence of liquid stools, and the presence of blood, mucus, or pus in the stools. Differences were noted in male and female defecation patterns and in the defecation frequencies of different age groups. Stool specimens for bacteriologic, virologic, and parasitologic examination were collected monthly on a regular basis and weekly when diarrhea occurred. Numerically, viruses were isolated and identified more often than other agents. The most commonly isolated parasite species and viral and bacterial serotypes were G. lamblia (from 222 subjects), echovirus 11 (from 166 subjects), and enteropathogenic Escherichia coli 026:B6 (from 138 subjects). Compared with the findings of several studies in other countries, isolations of shigellae were relatively rare.", "contents": "Diarrheal diseases of infancy in Cali, Colombia: study design and summary report on isolated disease agents. For public health reasons, it is important that the etiologic agents of early childhood diarrhea be isolated and identified, and that their routes of transmission be defined. This is especially true in tropical and subtropical developing countries, where childhood patterns of exposure to diarrheal disease agents usually differ from those in developed countries, and where diarrheal illness is a frequent harbinger of death among children under five years of age. This artical describes a study designed to identify diarrheal disease agents and transmission patterns in Cali, a large city of western Colombia's fertile Cauca River Valley. The study area, composed of five working-class districts with a total population of some 40,000, appeared to provide an environment fairly similar to those of many other \"average\" working-class communities in Latin America. Beginning in July 1962, a cohort of 296 children being born in these districts was studied, the period of investigation starting with the date of birth and continuing until each child's second birthday or its premature withdrawal from the study. Weekly home visits were made to establish defecation patterns, feeding practices, and anthropometry. The resulting data were then analyzed in terms of defecation frequencies, occurrence of liquid stools, and the presence of blood, mucus, or pus in the stools. Differences were noted in male and female defecation patterns and in the defecation frequencies of different age groups. Stool specimens for bacteriologic, virologic, and parasitologic examination were collected monthly on a regular basis and weekly when diarrhea occurred. Numerically, viruses were isolated and identified more often than other agents. The most commonly isolated parasite species and viral and bacterial serotypes were G. lamblia (from 222 subjects), echovirus 11 (from 166 subjects), and enteropathogenic Escherichia coli 026:B6 (from 138 subjects). Compared with the findings of several studies in other countries, isolations of shigellae were relatively rare.", "PMID": 974303}
{"id": "pubmed23n0032_9845", "title": "National service in rural areas: the case of Colombia.", "content": "Sending new medical school graduates into rural areas does give underserved populations access to modern medical care, perhaps for the first time. However, these young physicians usually spend most of their time providing strictly clinical services and are generally eager to leave at the end of their assignment. Some have referred to this practice as \"parachuting\" a doctor into an underserved area. An alternative is to focus first on building up permanent health service facilities in rural areas that are staffed by appropriate paraprofessionals. The young physician can then play a much more important role, serving as an administrator, researcher, evaluator, and teacher of the local staff; his clinical activities would generally be confined to the more complicated medical problems that arise. Such a model has been implemented in Kenya and Tanzania. An informal field assessment in Colombia has indicated that this model could be effectively applied there as well; indeed, it has been the hope of Colombian policymakers that the rural intern would function in such a way. However, this would require developing the nation's system of health services, as well as providing specific training aimed at preparing the medical student to perform his assigned role.", "contents": "National service in rural areas: the case of Colombia. Sending new medical school graduates into rural areas does give underserved populations access to modern medical care, perhaps for the first time. However, these young physicians usually spend most of their time providing strictly clinical services and are generally eager to leave at the end of their assignment. Some have referred to this practice as \"parachuting\" a doctor into an underserved area. An alternative is to focus first on building up permanent health service facilities in rural areas that are staffed by appropriate paraprofessionals. The young physician can then play a much more important role, serving as an administrator, researcher, evaluator, and teacher of the local staff; his clinical activities would generally be confined to the more complicated medical problems that arise. Such a model has been implemented in Kenya and Tanzania. An informal field assessment in Colombia has indicated that this model could be effectively applied there as well; indeed, it has been the hope of Colombian policymakers that the rural intern would function in such a way. However, this would require developing the nation's system of health services, as well as providing specific training aimed at preparing the medical student to perform his assigned role.", "PMID": 974304}
{"id": "pubmed23n0032_9846", "title": "Earthquake in Guatemala: epidemiologic evaluation of the relief effort.", "content": "The damage wrought by the earthquake that recently struck Guatemala evoked a commendable and efficient national response. It also led to international relief activity that was clearly instrumental in assisting the country's short-term recovery efforts. At the same time, this initial recovery period caused a good deal of experience to be gained in the field of disaster management. Some of the lessons derived from the experience are examined, and a number of approaches are suggested for measurably improving both national and international response to future disasters of this kind.", "contents": "Earthquake in Guatemala: epidemiologic evaluation of the relief effort. The damage wrought by the earthquake that recently struck Guatemala evoked a commendable and efficient national response. It also led to international relief activity that was clearly instrumental in assisting the country's short-term recovery efforts. At the same time, this initial recovery period caused a good deal of experience to be gained in the field of disaster management. Some of the lessons derived from the experience are examined, and a number of approaches are suggested for measurably improving both national and international response to future disasters of this kind.", "PMID": 974305}
{"id": "pubmed23n0032_9847", "title": "An irreversible effect of lithium administration to patients.", "content": "1 Lithium administration to patients leads to a pronounced inhibition (about 90%) of the choline transport system in erythrocytes. The transport system does not recover when ghosts are prepared from the erythrocytes, thereby removing intracellular as well as extracellular lithium. 2 When a patient is taken off lithium, the choline transport in erythrocytes recovers only very slowly over a period of three months, i.e. at about the same rate at which the erythrocytes that had been exposed to lithium are replaced by new cells. 3 It is concluded that therapeutic concentrations of lithium produce an irreversible inhibition of the choline transport system in human erythrocytes.", "contents": "An irreversible effect of lithium administration to patients. 1 Lithium administration to patients leads to a pronounced inhibition (about 90%) of the choline transport system in erythrocytes. The transport system does not recover when ghosts are prepared from the erythrocytes, thereby removing intracellular as well as extracellular lithium. 2 When a patient is taken off lithium, the choline transport in erythrocytes recovers only very slowly over a period of three months, i.e. at about the same rate at which the erythrocytes that had been exposed to lithium are replaced by new cells. 3 It is concluded that therapeutic concentrations of lithium produce an irreversible inhibition of the choline transport system in human erythrocytes.", "PMID": 974314}
{"id": "pubmed23n0032_9848", "title": "Depression by morphine of excitatory junction potentials in the vas deferens of the mouse.", "content": "1 Intracellular recordings were made from smooth muscle cells of the mouse vas deferens. Excitatory junction potentials (e.j.ps) were evoked by stimulation of the intramural nerves. 2 Normorphine (50 nM-5muM) depressed the amplitude of the e.j.p. The ED50 was 430 nM. The latency of the e.j.p. and the resting membrane potential of the smooth muscle cells were unaffected by normorphine. 3 The depression of the e.j.p. by narcotic analgesic drugs was stereospecific. 4 Naloxone (100 nM) completely reversed the depression of the e.j.p. produced by normorphine (1 muM). Naloxone (100 nM) alone did not alter the amplitude of the e.j.p. 5 Normorphine (1 muM) did not prevent the depolarization of the smooth muscle cells produced by exogenous noradrenaline (10 muM). 6 It is concluded that narcotic analgesic drugs act directly upon the transmitter release sites to reduce the amount of noradrenaline liberated by each nerve impulse.", "contents": "Depression by morphine of excitatory junction potentials in the vas deferens of the mouse. 1 Intracellular recordings were made from smooth muscle cells of the mouse vas deferens. Excitatory junction potentials (e.j.ps) were evoked by stimulation of the intramural nerves. 2 Normorphine (50 nM-5muM) depressed the amplitude of the e.j.p. The ED50 was 430 nM. The latency of the e.j.p. and the resting membrane potential of the smooth muscle cells were unaffected by normorphine. 3 The depression of the e.j.p. by narcotic analgesic drugs was stereospecific. 4 Naloxone (100 nM) completely reversed the depression of the e.j.p. produced by normorphine (1 muM). Naloxone (100 nM) alone did not alter the amplitude of the e.j.p. 5 Normorphine (1 muM) did not prevent the depolarization of the smooth muscle cells produced by exogenous noradrenaline (10 muM). 6 It is concluded that narcotic analgesic drugs act directly upon the transmitter release sites to reduce the amount of noradrenaline liberated by each nerve impulse.", "PMID": 974315}
{"id": "pubmed23n0032_9849", "title": "The cholinergic component in the reflex vasodilatation elicited by stimulation of the depressor nerves in the rabbit.", "content": "1 The effects of the stimulation of the cephalic endings of the depressor nerve on the resistance in the perfused hindlimb were studied in the rabbit. 2 The vasodilatation thus elicited in the perfused hindlimb was reduced either by administration of guanethidine or by sympathectomy and abolished by subsequent treatment with atropine. 3 These data confirm the existence of two components in the genesis of the reflex vasodilatation: a passive component, due to the inhibition of sympathetic discharge, and an active component which in the rabbit is cholinergic in nature.", "contents": "The cholinergic component in the reflex vasodilatation elicited by stimulation of the depressor nerves in the rabbit. 1 The effects of the stimulation of the cephalic endings of the depressor nerve on the resistance in the perfused hindlimb were studied in the rabbit. 2 The vasodilatation thus elicited in the perfused hindlimb was reduced either by administration of guanethidine or by sympathectomy and abolished by subsequent treatment with atropine. 3 These data confirm the existence of two components in the genesis of the reflex vasodilatation: a passive component, due to the inhibition of sympathetic discharge, and an active component which in the rabbit is cholinergic in nature.", "PMID": 974316}
{"id": "pubmed23n0032_9850", "title": "Uterine receptors for oxytocin: correlation between antagonist potency and receptor binding.", "content": "1 The apparent dissociation constants (Kd) of four competitive antagonists of oxytocin were estimated from their ability to compete with [3H]-oxytocin for binding sites in particulate fractions from rat uterine homogenates. 2 These apparent Kd values were not significantly different from the Kd values calculated from the published potency of each compound as an antagonist of oxytocin-induced uterine contractions. 3 These results support the conclusion that the binding sites for oxytocin are part of the receptor complex. Furthermore, 'spare receptors' for oxytocin do not appear to be present in significant quantities, and the relative potency of each antagonist appears to depend upon its affinity for the receptor site rather than its intrinsic activity. 4 The antagonists used in these studies were [N-acetyl, 2-O-methyltyrosine]oxytocin, [1-(beta-mercapto-beta,beta-diethylpropionic acid)]oxytocin, [1-(beta-mercapto-beta,beta-pentamethylenepropionic acid)]oxytocin, and [1-(deaminopenicillamine), 4-threonine]oxytocin.", "contents": "Uterine receptors for oxytocin: correlation between antagonist potency and receptor binding. 1 The apparent dissociation constants (Kd) of four competitive antagonists of oxytocin were estimated from their ability to compete with [3H]-oxytocin for binding sites in particulate fractions from rat uterine homogenates. 2 These apparent Kd values were not significantly different from the Kd values calculated from the published potency of each compound as an antagonist of oxytocin-induced uterine contractions. 3 These results support the conclusion that the binding sites for oxytocin are part of the receptor complex. Furthermore, 'spare receptors' for oxytocin do not appear to be present in significant quantities, and the relative potency of each antagonist appears to depend upon its affinity for the receptor site rather than its intrinsic activity. 4 The antagonists used in these studies were [N-acetyl, 2-O-methyltyrosine]oxytocin, [1-(beta-mercapto-beta,beta-diethylpropionic acid)]oxytocin, [1-(beta-mercapto-beta,beta-pentamethylenepropionic acid)]oxytocin, and [1-(deaminopenicillamine), 4-threonine]oxytocin.", "PMID": 974317}
{"id": "pubmed23n0032_9851", "title": "The use of different agonists in antagonist affinity constant estimations.", "content": "1 The affinities of 4 muscarinic antagonists were estimated on intact pieces of guinea-pig ileum using the agonists carbachol and pentyl trimethylammonium both in separate experiments and in the same experiment. 2 The apparent affinities were slightly but consistently higher when estimated from the responses produced by pentyl trimethylammonium than when estimated from the responses produced by carbachol. 3 This difference was greatly reduced or abolished if totally denervated logitudinal muscle strips were used rather than intact pieces of ileum. It is therefore suggested that the difference is due to the presence of receptors in the ganglionic layer. 4 To explain the difference in apparent affinity of the antagonists these receptors can not be identical to the muscarinic receptors on the smooth muscle. 5 In addition they can not be nicotinic ganglionic receptors as the difference did not appear to be affected by the presence or absence of hexamethonium.", "contents": "The use of different agonists in antagonist affinity constant estimations. 1 The affinities of 4 muscarinic antagonists were estimated on intact pieces of guinea-pig ileum using the agonists carbachol and pentyl trimethylammonium both in separate experiments and in the same experiment. 2 The apparent affinities were slightly but consistently higher when estimated from the responses produced by pentyl trimethylammonium than when estimated from the responses produced by carbachol. 3 This difference was greatly reduced or abolished if totally denervated logitudinal muscle strips were used rather than intact pieces of ileum. It is therefore suggested that the difference is due to the presence of receptors in the ganglionic layer. 4 To explain the difference in apparent affinity of the antagonists these receptors can not be identical to the muscarinic receptors on the smooth muscle. 5 In addition they can not be nicotinic ganglionic receptors as the difference did not appear to be affected by the presence or absence of hexamethonium.", "PMID": 974318}
{"id": "pubmed23n0032_9852", "title": "The pharmacology of anaphylaxis in the chicken intestine.", "content": "1 The Schultz-Dale phenomenon has been demonstrated in several circular smooth muscle strips of oesophagus, crop, duodenum, jejunum and ileum taken from young and adult domestic fowl sensitized actively to crystalline bovine albumin or horse plasma. 2 The ileal strips contract to acetylcholine, histamine, 5-hydroxytryptamine (5-HT), prostaglandins E1, E2, F2alpha, bradykinin and bovine slow reacting substance of anaphylaxis (SRS-A). Marked seasonal and individual variations in the responsiveness of gut tissues to these exogenous agonists were noted. 3 Antagonism of contractions to histamine by mepyramine suggests the existence of H1-histamine receptors in chicken ileum. Blockade of 5-HT-induced contractions by methysergide shows the preponderance of 'D'-musculotropic tryptamine receptors. 4 Failure of selective receptor antagonists of acetylcholine, histamine and 5-HT to modify the Schultz-Dale reaction suggests the nonparticipation of aminergic mechanisms in this reaction. 5 Partial to complete blockade of the Schultz-Dale reaction by a prostaglandin receptor antagonist (polyphloretin phosphate, PPP); prostaglandin synthetase inhibitors (sodium meclofenamate and phenylbutazone); inhibitors of synthesis and release of histamine and SRS-A (PR-D-92-EA, M & B 22948, diethylcarbamazine citrate, and PPP) and an inhibitor of proteinases (aprotinin) strongly suggests the involvement of vasoactive lipids and polypeptides in the anaphylactic response of chicken ileum to specific antigen.", "contents": "The pharmacology of anaphylaxis in the chicken intestine. 1 The Schultz-Dale phenomenon has been demonstrated in several circular smooth muscle strips of oesophagus, crop, duodenum, jejunum and ileum taken from young and adult domestic fowl sensitized actively to crystalline bovine albumin or horse plasma. 2 The ileal strips contract to acetylcholine, histamine, 5-hydroxytryptamine (5-HT), prostaglandins E1, E2, F2alpha, bradykinin and bovine slow reacting substance of anaphylaxis (SRS-A). Marked seasonal and individual variations in the responsiveness of gut tissues to these exogenous agonists were noted. 3 Antagonism of contractions to histamine by mepyramine suggests the existence of H1-histamine receptors in chicken ileum. Blockade of 5-HT-induced contractions by methysergide shows the preponderance of 'D'-musculotropic tryptamine receptors. 4 Failure of selective receptor antagonists of acetylcholine, histamine and 5-HT to modify the Schultz-Dale reaction suggests the nonparticipation of aminergic mechanisms in this reaction. 5 Partial to complete blockade of the Schultz-Dale reaction by a prostaglandin receptor antagonist (polyphloretin phosphate, PPP); prostaglandin synthetase inhibitors (sodium meclofenamate and phenylbutazone); inhibitors of synthesis and release of histamine and SRS-A (PR-D-92-EA, M & B 22948, diethylcarbamazine citrate, and PPP) and an inhibitor of proteinases (aprotinin) strongly suggests the involvement of vasoactive lipids and polypeptides in the anaphylactic response of chicken ileum to specific antigen.", "PMID": 974319}
{"id": "pubmed23n0032_9853", "title": "Adenosine on myocardial oxygen consumption.", "content": "1 A 3 min intracoronary infusion of adenosine (50 mug/min) produced a significant decrease in coronary artery perfusion pressure, left ventricular systolic pressure and myocardial O2 consumption in the isolated supported heart preparation of the dog perfused at a constant coronary blood flow. Heart rate was controlled at 150, 190 or 230 beats/minute. 2 Myocardial contractile force and maximal left ventricular dp/dt were not changed by adenosine infusion. 3 The absolute decrease in myocardial O2 consumption was greater at increasing heart rates whereas the decrease in coronary artery perfusion pressure and peak left ventricular systolic pressure were similar. 4 The results suggest that the reduction in myocardial O2 consumption produced by adenosine is not related to coronary vasodilatation or to a negative chronotropic or inotropic action, but may be due to a functional shunting of blood flow from high O2 extracting regions of the myocardium to low O2 extracting ones and/or important effects on myocardial substrate utilization.", "contents": "Adenosine on myocardial oxygen consumption. 1 A 3 min intracoronary infusion of adenosine (50 mug/min) produced a significant decrease in coronary artery perfusion pressure, left ventricular systolic pressure and myocardial O2 consumption in the isolated supported heart preparation of the dog perfused at a constant coronary blood flow. Heart rate was controlled at 150, 190 or 230 beats/minute. 2 Myocardial contractile force and maximal left ventricular dp/dt were not changed by adenosine infusion. 3 The absolute decrease in myocardial O2 consumption was greater at increasing heart rates whereas the decrease in coronary artery perfusion pressure and peak left ventricular systolic pressure were similar. 4 The results suggest that the reduction in myocardial O2 consumption produced by adenosine is not related to coronary vasodilatation or to a negative chronotropic or inotropic action, but may be due to a functional shunting of blood flow from high O2 extracting regions of the myocardium to low O2 extracting ones and/or important effects on myocardial substrate utilization.", "PMID": 974320}
{"id": "pubmed23n0032_9854", "title": "Decreased phenylethanolamine-N-methyltransferase and catechol-O-methyltransferase activity in rabbit adrenal glands during pregnancy.", "content": "1 Phenylethanolamine-N-methyltransferase (PNMT) activity in adrenal gland was lower than the control values at day 24 of rabbit pregnancy and fell throughout the gestational period. 2 Catechol-O-methyltransferase (COMT) activity during pregnancy and parturition followed a similar pattern to PNMT. 3 Adrenaline content of the adrenal gland declined significantly during the last few days of pregnancy but at parturition showed a tendency to return towards normal. 4 The biochemical mechanisms which result in a decline in PNMT and COMT activities and adrenaline concentration appear to be the consequence of modifications in endocrine gland secretion during pregnancy.", "contents": "Decreased phenylethanolamine-N-methyltransferase and catechol-O-methyltransferase activity in rabbit adrenal glands during pregnancy. 1 Phenylethanolamine-N-methyltransferase (PNMT) activity in adrenal gland was lower than the control values at day 24 of rabbit pregnancy and fell throughout the gestational period. 2 Catechol-O-methyltransferase (COMT) activity during pregnancy and parturition followed a similar pattern to PNMT. 3 Adrenaline content of the adrenal gland declined significantly during the last few days of pregnancy but at parturition showed a tendency to return towards normal. 4 The biochemical mechanisms which result in a decline in PNMT and COMT activities and adrenaline concentration appear to be the consequence of modifications in endocrine gland secretion during pregnancy.", "PMID": 974321}
{"id": "pubmed23n0032_9855", "title": "Morphine-naloxone interaction in the central cholinergic system: the influence of subcortical lesioning and electrical stimulation.", "content": "1 The opiate antagonist naloxone, injected or topically applied to the cerebral cortex, had no significant effect on the spontaneous output of cortical acetylcholine (ACh) in rats. 2 Morphine (2.5 mg/kg) administered intravenously inhibited the release of cortical ACh. A subsequent injection of naloxone rapidly reversed morphine-induced inhibition, and produced a sustained increase in the release of ACh. Topical application of naloxone solutions, after morphine, produced a slow and weak reversal of its inhibitory action. 3 Destruction of the medial thalamus abolished both the inhibitory effects of morphine on the cortical ACh release, and its antagonism by naloxone administered after the agonist. 4 Injection of naloxone in a low dose (0.1 mg/kg) increased the release of cortical ACh provoked by electrical stimulation of either the medial thalamus or the reticular formation in normal rats. In the morphine-dependent rat, naloxone also facilitated the evoked release and its action was greater than in control animals. The facilitatory effect of naloxone on the cortical release evoked by stimulation of the medial thalamus was greater than its effect on the release evoked by stimulation of the reticular formation in both normal and morphine-dependent rats. 5 Naltrexone, a narcotic antagonist, also facilitated the electrically stimulated release of cortical ACh. 6 It is suggested that (a) morphine and naloxone act at a subcortical site, probably the medial thalamus, to modify the cortical ACh release and that (b) naloxone may facilitate the electrically-induced release of ACh in the CNS by antagonizing the effect of the endogenous morphine-like factor, enkephalin.", "contents": "Morphine-naloxone interaction in the central cholinergic system: the influence of subcortical lesioning and electrical stimulation. 1 The opiate antagonist naloxone, injected or topically applied to the cerebral cortex, had no significant effect on the spontaneous output of cortical acetylcholine (ACh) in rats. 2 Morphine (2.5 mg/kg) administered intravenously inhibited the release of cortical ACh. A subsequent injection of naloxone rapidly reversed morphine-induced inhibition, and produced a sustained increase in the release of ACh. Topical application of naloxone solutions, after morphine, produced a slow and weak reversal of its inhibitory action. 3 Destruction of the medial thalamus abolished both the inhibitory effects of morphine on the cortical ACh release, and its antagonism by naloxone administered after the agonist. 4 Injection of naloxone in a low dose (0.1 mg/kg) increased the release of cortical ACh provoked by electrical stimulation of either the medial thalamus or the reticular formation in normal rats. In the morphine-dependent rat, naloxone also facilitated the evoked release and its action was greater than in control animals. The facilitatory effect of naloxone on the cortical release evoked by stimulation of the medial thalamus was greater than its effect on the release evoked by stimulation of the reticular formation in both normal and morphine-dependent rats. 5 Naltrexone, a narcotic antagonist, also facilitated the electrically stimulated release of cortical ACh. 6 It is suggested that (a) morphine and naloxone act at a subcortical site, probably the medial thalamus, to modify the cortical ACh release and that (b) naloxone may facilitate the electrically-induced release of ACh in the CNS by antagonizing the effect of the endogenous morphine-like factor, enkephalin.", "PMID": 974367}
{"id": "pubmed23n0032_9856", "title": "The effect of catecholamines on the influx of calcium and the development of tension in denervated mouse diaphragm muscle.", "content": "1 The nature of the catecholamine-induced contracture of chronically denervated mouse diaphragm muscle has been investigated and compared with the contractural response evoked by acetylcholine. 2 The time course of onset of catecholamine-sensitivity in denervated diaphragm muscles was similar to the development of acetylcholine sensitivity. However, catecholamine contractures were absent in tissues denervated for periods longer than 90 days whereas acetylcholine-sensitivity was still evident several months after denervation. 3 The catecholamine-induced contracture of the denervated muscle was inhibited specifically by beta-receptor blocking drugs and was unaffected by alpha-receptor blocking drugs and cholinoceptor antagonists. 4 Catecholamine-induced contractures of denervated muscles, unlike contractures to acetylcholine, were dependent upon the presence of spontaneous fibrillation and the amplitude of spontaneous fibrillation was increased by catecholamines. Fibrillation was absent in the presence of tetrodotoxin (1 muM), 2,4-dinitrophenol (10 muM), potassium cyanide (10 muM), ouabain (100 muM), in lithium chloride Ringer solution and at low temperature. Under these conditions catecholamine-induced contractures, but not those to acetylcholine, were abolished. 5 Labelled calcium was found progressively to enter denervated muscle fibres and this entry of calcium was increased by catecholamines. It is suggested that this calcium entry may represent either an increased calcium permeability of denervated muscle fibres which is increased further by catecholamines or the presence of a calcium current that occurs during the fibrillatory potentials of denervated muscle.", "contents": "The effect of catecholamines on the influx of calcium and the development of tension in denervated mouse diaphragm muscle. 1 The nature of the catecholamine-induced contracture of chronically denervated mouse diaphragm muscle has been investigated and compared with the contractural response evoked by acetylcholine. 2 The time course of onset of catecholamine-sensitivity in denervated diaphragm muscles was similar to the development of acetylcholine sensitivity. However, catecholamine contractures were absent in tissues denervated for periods longer than 90 days whereas acetylcholine-sensitivity was still evident several months after denervation. 3 The catecholamine-induced contracture of the denervated muscle was inhibited specifically by beta-receptor blocking drugs and was unaffected by alpha-receptor blocking drugs and cholinoceptor antagonists. 4 Catecholamine-induced contractures of denervated muscles, unlike contractures to acetylcholine, were dependent upon the presence of spontaneous fibrillation and the amplitude of spontaneous fibrillation was increased by catecholamines. Fibrillation was absent in the presence of tetrodotoxin (1 muM), 2,4-dinitrophenol (10 muM), potassium cyanide (10 muM), ouabain (100 muM), in lithium chloride Ringer solution and at low temperature. Under these conditions catecholamine-induced contractures, but not those to acetylcholine, were abolished. 5 Labelled calcium was found progressively to enter denervated muscle fibres and this entry of calcium was increased by catecholamines. It is suggested that this calcium entry may represent either an increased calcium permeability of denervated muscle fibres which is increased further by catecholamines or the presence of a calcium current that occurs during the fibrillatory potentials of denervated muscle.", "PMID": 974368}
{"id": "pubmed23n0032_9857", "title": "Effects of L-tryptophan and L-methionine on activity in the rat.", "content": "1 The effects of an intraperitoneal dose of 20 mg/kg L-tryptophan and doses of 100 and 150 mg/kg of L-methionine were investigated on activity in the rat. 2 Activity was measured by a time sampling behaviour categorization procedure, and began 15 min after compound administration, lasting for a total of one hour. 3 Total active behaviour in the first half hour was reduced after 20 mg/kg tryptophan, 100 or 150 mg/kg methionine. Only 20 mg/kg tryptophan led to a significant reduction in active behaviour during the second half hour. 4 Administration of 100 mg/kg methionine plus 20 mg/kg tryptophan, and 150 mg/kg methionine plus 20 mg/kg tryptophan had no discernible effect on activity. 5 The results draw attention to the role of another amino acid in the modification of the behavioural effects of tryptophan.", "contents": "Effects of L-tryptophan and L-methionine on activity in the rat. 1 The effects of an intraperitoneal dose of 20 mg/kg L-tryptophan and doses of 100 and 150 mg/kg of L-methionine were investigated on activity in the rat. 2 Activity was measured by a time sampling behaviour categorization procedure, and began 15 min after compound administration, lasting for a total of one hour. 3 Total active behaviour in the first half hour was reduced after 20 mg/kg tryptophan, 100 or 150 mg/kg methionine. Only 20 mg/kg tryptophan led to a significant reduction in active behaviour during the second half hour. 4 Administration of 100 mg/kg methionine plus 20 mg/kg tryptophan, and 150 mg/kg methionine plus 20 mg/kg tryptophan had no discernible effect on activity. 5 The results draw attention to the role of another amino acid in the modification of the behavioural effects of tryptophan.", "PMID": 974369}
{"id": "pubmed23n0032_9858", "title": "Influence of dopamine and noradrenaline on isolated cerebral arteries of the dog.", "content": "1 Effects of dopamine and noradrenaline were compared in helically-cut strips of canine cerebral arteries. 2 Dopamine caused a greater maximal contraction than noradrenaline, although the ED50 for noradrenaline was appreciably less. The contraction induced by these amines was reversed to a relaxation by treatment with phenoxybenzamine. 3 Relaxation induced by dopamine in phenoxybenzamine-treated and prostaglandin-contracted cerebral arteries was not influenced by 1 muM propranolol, while relaxation induced by noradrenaline at low concentrations (2 muM and 10 muM) was significantly attenuated. Neither aminophylline nor atropine affected the relaxant effect of dopamine. 4 A mechanism other than beta-adrenergic, cholinergic or adenosine-related appears to be involved in the relaxation elicited by dopamine in cerebral arterial strips.", "contents": "Influence of dopamine and noradrenaline on isolated cerebral arteries of the dog. 1 Effects of dopamine and noradrenaline were compared in helically-cut strips of canine cerebral arteries. 2 Dopamine caused a greater maximal contraction than noradrenaline, although the ED50 for noradrenaline was appreciably less. The contraction induced by these amines was reversed to a relaxation by treatment with phenoxybenzamine. 3 Relaxation induced by dopamine in phenoxybenzamine-treated and prostaglandin-contracted cerebral arteries was not influenced by 1 muM propranolol, while relaxation induced by noradrenaline at low concentrations (2 muM and 10 muM) was significantly attenuated. Neither aminophylline nor atropine affected the relaxant effect of dopamine. 4 A mechanism other than beta-adrenergic, cholinergic or adenosine-related appears to be involved in the relaxation elicited by dopamine in cerebral arterial strips.", "PMID": 974370}
{"id": "pubmed23n0032_9859", "title": "The actions of natural secretin on the small intestinal vasculature of the anaesthetized cat.", "content": "1 A plethysmographic preparation of cat jejunum was used to measure changes in tissue volume and capillary filtration coefficient (CFC), simultaneously with measurements of arterial and venous pressures, and total blood flow. 2 Secretin was infused and injected intravenously and also infused intra-arterially in relatively small doses. Probable resulting blood concentrations were compared with those determined under physiological conditions in other investigations. 3 By intravenous or intra-arterial infusion, secretin caused increases in CFC, indicating an increased functional exchange vessel area, and increases in jejunal volume, indicating increased vascular capacitance. The jejunal blood flow increased whilst the blood pressure remained essentially unchanged. 4 By intravenous injection, secretin caused rises in jejunal volume and reductions in calculated jejunal vascular resistance over the same dose range. Effects were statistically significant at 500 mu/kg and higher doses caused reductions in systemic arterial pressure. 5 The calculated peak blood concentrations of secretin resulting from the lower doses used in this investigation were of the same order of magnitude as those determined under physiological conditions in man. 6 It is possible that at physiological concentrations secretin causes an increased functional exchange vessel area in the small intestine, and may also increase the total blood flow through this tissue.", "contents": "The actions of natural secretin on the small intestinal vasculature of the anaesthetized cat. 1 A plethysmographic preparation of cat jejunum was used to measure changes in tissue volume and capillary filtration coefficient (CFC), simultaneously with measurements of arterial and venous pressures, and total blood flow. 2 Secretin was infused and injected intravenously and also infused intra-arterially in relatively small doses. Probable resulting blood concentrations were compared with those determined under physiological conditions in other investigations. 3 By intravenous or intra-arterial infusion, secretin caused increases in CFC, indicating an increased functional exchange vessel area, and increases in jejunal volume, indicating increased vascular capacitance. The jejunal blood flow increased whilst the blood pressure remained essentially unchanged. 4 By intravenous injection, secretin caused rises in jejunal volume and reductions in calculated jejunal vascular resistance over the same dose range. Effects were statistically significant at 500 mu/kg and higher doses caused reductions in systemic arterial pressure. 5 The calculated peak blood concentrations of secretin resulting from the lower doses used in this investigation were of the same order of magnitude as those determined under physiological conditions in man. 6 It is possible that at physiological concentrations secretin causes an increased functional exchange vessel area in the small intestine, and may also increase the total blood flow through this tissue.", "PMID": 974371}
{"id": "pubmed23n0032_9860", "title": "Labelled decamethonium in cat muscle.", "content": "1 Tritium-labelled decamethonium was infused intravenously in 12 cats at final rates of 1.3-4.2 nmol kg-1 min-1 to produce a steady plasma concentration which ranged between 0.21-1.3 mumol/l in different experiments. Muscle contractions were elicited by nerve stimulation and the potential at the end-plate regions of superficial fibres was recorded by extracellular electrodes. 2 Under these conditions, it was not possible to obtain a steady degree of neuromuscular block. The initial decrease in muscle contractions was followed by recovery towards the original value although the concentration of decamethonium in the plasma remained constant, or in some cases rose. The initial depolarization of the end-plate region also waned during the constant infusion of the drug. 3 Once the twitch tension had returned to control values during infusion of the drug, prolongation of the infusion for a total of four hours did not produce a secondary neuromuscular block. 4 Scintillation counting showed that during infusion of labelled decamethonium the radioactivity of the muscles increased progressively with time. The uptake was less in the soleus muscle than in the fast-contracting flexor longus digitorum and extensor longus digitorum muscles. Muscles which had been denervated 12-13 days previously showed a greater uptake of labelled drug than control muscles from the contralateral limb. 5 The labelled drug was localized by autoradiography of frozen sections of leg muscles following intra-arterial injection of decamethonium. Grain counts in individual fibres showed that small amounts of decamethonium had entered the muscle fibres along their entire length, and there was increased uptake of the drug into the cell in the region of the end-plate. 6 The mechanisms underlying the waning of the pharmacological response during constant application of depolarizing drugs are discussed.", "contents": "Labelled decamethonium in cat muscle. 1 Tritium-labelled decamethonium was infused intravenously in 12 cats at final rates of 1.3-4.2 nmol kg-1 min-1 to produce a steady plasma concentration which ranged between 0.21-1.3 mumol/l in different experiments. Muscle contractions were elicited by nerve stimulation and the potential at the end-plate regions of superficial fibres was recorded by extracellular electrodes. 2 Under these conditions, it was not possible to obtain a steady degree of neuromuscular block. The initial decrease in muscle contractions was followed by recovery towards the original value although the concentration of decamethonium in the plasma remained constant, or in some cases rose. The initial depolarization of the end-plate region also waned during the constant infusion of the drug. 3 Once the twitch tension had returned to control values during infusion of the drug, prolongation of the infusion for a total of four hours did not produce a secondary neuromuscular block. 4 Scintillation counting showed that during infusion of labelled decamethonium the radioactivity of the muscles increased progressively with time. The uptake was less in the soleus muscle than in the fast-contracting flexor longus digitorum and extensor longus digitorum muscles. Muscles which had been denervated 12-13 days previously showed a greater uptake of labelled drug than control muscles from the contralateral limb. 5 The labelled drug was localized by autoradiography of frozen sections of leg muscles following intra-arterial injection of decamethonium. Grain counts in individual fibres showed that small amounts of decamethonium had entered the muscle fibres along their entire length, and there was increased uptake of the drug into the cell in the region of the end-plate. 6 The mechanisms underlying the waning of the pharmacological response during constant application of depolarizing drugs are discussed.", "PMID": 974372}
{"id": "pubmed23n0032_9861", "title": "Action of surugatoxin on nicotinic receptors in the superior cervical ganglion of the rat.", "content": "Surugatoxin (SGTX, 0.1-2 muM) reversibly depressed orthodromic transmission and antagonized the depolarizing action of carbachol on the isolated superior cervical ganglion of the rat. The apparent dissociation equilibrium constant against carbachol-induced depolarization (measured in the presence of hyoscine) was 58 and 76 nM determined at 0.2 and 2 muM respectively. SGTX (2muM) did not reduce the depolarizing effects of (+/-)-muscarine, gamma-aminobutyric acid or angiotensin, but did reduce that to 5-hydroxytryptamine. Release of [3H]-acetylcholine following repetitive (10 Hz) preganglionic sympathetic stimulation was maintained in the presence of 2 muM SGTX. It is concluded that SGTX has a high and selective affinity for ganglionic nicotinic receptors.", "contents": "Action of surugatoxin on nicotinic receptors in the superior cervical ganglion of the rat. Surugatoxin (SGTX, 0.1-2 muM) reversibly depressed orthodromic transmission and antagonized the depolarizing action of carbachol on the isolated superior cervical ganglion of the rat. The apparent dissociation equilibrium constant against carbachol-induced depolarization (measured in the presence of hyoscine) was 58 and 76 nM determined at 0.2 and 2 muM respectively. SGTX (2muM) did not reduce the depolarizing effects of (+/-)-muscarine, gamma-aminobutyric acid or angiotensin, but did reduce that to 5-hydroxytryptamine. Release of [3H]-acetylcholine following repetitive (10 Hz) preganglionic sympathetic stimulation was maintained in the presence of 2 muM SGTX. It is concluded that SGTX has a high and selective affinity for ganglionic nicotinic receptors.", "PMID": 974373}
{"id": "pubmed23n0032_9862", "title": "Further studies on the mode of action of isoprenaline on gastric secretion in the conscious rat.", "content": "1 The effect of isoprenaline on gastric secretion evoked by various means has been studied in conscious rats provided with Pavlov and Heidenhain pouches. 2 Interdigestive acid secretion in the Pavlov pouch was reduced by isoprenaline, whereas pepsin secretion was unaltered. 3 Central vagal stimulation effected by 2-deoxy-D-glucose injection evoked a gastric secretory response that was substantially reduced by isoprenaline. 4 2-Deoxy-D-glucose increased the mobilization of gastric mucosal histamine, an effect that was prevented by isoprenaline. 5 Isoprenaline infusion alone induced a slight increase in histamine mobilization and also a considerable elevation of immunoreactive serum gastrin concentration. 6 The secretory response to food in the Pavlov pouch was almost abolished by isoprenaline. 7 Although the acid response to histamine in the Heidenhain pouch was susceptible to isoprenaline inhibition, that to methacholine was not. 8 Pepsin secretion in the Heidenhain pouch preparation stimulated by histamine or methacholine seemed to be enhanced by isoprenaline.", "contents": "Further studies on the mode of action of isoprenaline on gastric secretion in the conscious rat. 1 The effect of isoprenaline on gastric secretion evoked by various means has been studied in conscious rats provided with Pavlov and Heidenhain pouches. 2 Interdigestive acid secretion in the Pavlov pouch was reduced by isoprenaline, whereas pepsin secretion was unaltered. 3 Central vagal stimulation effected by 2-deoxy-D-glucose injection evoked a gastric secretory response that was substantially reduced by isoprenaline. 4 2-Deoxy-D-glucose increased the mobilization of gastric mucosal histamine, an effect that was prevented by isoprenaline. 5 Isoprenaline infusion alone induced a slight increase in histamine mobilization and also a considerable elevation of immunoreactive serum gastrin concentration. 6 The secretory response to food in the Pavlov pouch was almost abolished by isoprenaline. 7 Although the acid response to histamine in the Heidenhain pouch was susceptible to isoprenaline inhibition, that to methacholine was not. 8 Pepsin secretion in the Heidenhain pouch preparation stimulated by histamine or methacholine seemed to be enhanced by isoprenaline.", "PMID": 974374}
{"id": "pubmed23n0032_9863", "title": "A comparison of D and L-tryptophan on the cerebral metabolism of 5-hydroxytryptamine and dopamine in the dog.", "content": "1 After D and L-tryptophan (50 mg/kg) were given intravenously in the dog, the concentration of the amino acid was increased in ventricular cerebrospinal fluid (CSF) during the subsequent 4 h or sampling, although the concentrations were significantly lower following the administration of the D-isomer. 2 There was no evidence that D-tryptophan increased the synthesis of 5-hydroxytryptamine (5-HT) in dog brain as judged by the failure to cause a change in 5-hydroxyindoleacetic acid (5-HIAA) concentrations in ventricular CSF different from that seen with controls. 3 There was no appreciable conversion of D-tryptophan to L-tryptophan in the dog. 4 D-tryptophan was cleared more rapidly from plasma than L-tryptophan. 5 No difference in plasma binding between D and L-tryptophan was detected.", "contents": "A comparison of D and L-tryptophan on the cerebral metabolism of 5-hydroxytryptamine and dopamine in the dog. 1 After D and L-tryptophan (50 mg/kg) were given intravenously in the dog, the concentration of the amino acid was increased in ventricular cerebrospinal fluid (CSF) during the subsequent 4 h or sampling, although the concentrations were significantly lower following the administration of the D-isomer. 2 There was no evidence that D-tryptophan increased the synthesis of 5-hydroxytryptamine (5-HT) in dog brain as judged by the failure to cause a change in 5-hydroxyindoleacetic acid (5-HIAA) concentrations in ventricular CSF different from that seen with controls. 3 There was no appreciable conversion of D-tryptophan to L-tryptophan in the dog. 4 D-tryptophan was cleared more rapidly from plasma than L-tryptophan. 5 No difference in plasma binding between D and L-tryptophan was detected.", "PMID": 974375}
{"id": "pubmed23n0032_9864", "title": "Circulatory effects of oral and subcutaneous administration in normal subjects of a new bronchodilator, ibuterol, a pro-drug to terbutaline.", "content": "1 The di-isobutyryl ester of terbutaline, ibuterol, was given subcutaneously and orally to healthy volunteers and its effects on heart rate, arterial blood pressure, and ECG were compared with those of terbutaline itself. The effects of the two substances were qualitatively identical, but quantitatively different. 2 By mouth, ibuterol acted more rapidly, and was 2-3 times more active. After intramuscular injection, ibuterol caused less increase in local blood flow than terbutaline. 3 Ibuterol can be regarded as a pro-drug, which in itself has little activity, but which can be made active in the body.", "contents": "Circulatory effects of oral and subcutaneous administration in normal subjects of a new bronchodilator, ibuterol, a pro-drug to terbutaline. 1 The di-isobutyryl ester of terbutaline, ibuterol, was given subcutaneously and orally to healthy volunteers and its effects on heart rate, arterial blood pressure, and ECG were compared with those of terbutaline itself. The effects of the two substances were qualitatively identical, but quantitatively different. 2 By mouth, ibuterol acted more rapidly, and was 2-3 times more active. After intramuscular injection, ibuterol caused less increase in local blood flow than terbutaline. 3 Ibuterol can be regarded as a pro-drug, which in itself has little activity, but which can be made active in the body.", "PMID": 974376}
{"id": "pubmed23n0032_9865", "title": "The action of tetraethyl-ammonium chloride on the response of the rat anococcygeus muscle to motor and inhibitory nerve stimulation and to some drugs.", "content": "1 Tetraethylammonium chloride (TEA) 0.125 mM to 20 mM potentiates the response of the anococcygeus muscle to field stimulation of the motor adrenergic nerves without affecting the response to noradrenaline suggesting a pre-synaptic origin of potentiation. The potentiation is greatest at low, submaximal, frequencies (2 Hz) of stimulation and only slight at the higher frequency of 20 Hz. This difference is due to the restraint imposed on the demonstration of potentiation by maximal or near maximal motor responses since reduction of the mechanical response at 20 Hz by either phentolamine (post-synaptic block) or guanethidine (pre-synaptic block) resulted in a great increase in potentiation of the response at this frequency. 2 TEA in concentrations up to 1 mM similarly potentites the response to inhibitory nerve stimulation and again the greatest effect is at low frequencies. Higher concentrations (5-20 mM) progressively depress the inhibitory response. It is suggested that TEA may specifically antagonize the post-synaptic action of the inhibitory transmitter and that at higher concentrations of TEA this effect dominates the pre-synaptic action in increasing transmitter release. 3 TEA has no effect on the motor response to tyramine. 4 TEA (5-20 mM) causes a maintained rise in muscle tone. Part of this is abolished by phentolamine but part is resistant. A similar muscle stimulant action of TEA is observed in muscles from rats previously treated with 6-hydroxydopamine in which indirect sympathomimetic drugs and field stimulation could no longer produce a motor response. These results suggest that part of the motor effect of TEA is due to an increased spontaneous release of noradrenaline and part to a direct action on the muscle. 5 TEA 0.125 mM to 20 mM antagonize the stimulant action of carbachol. Dose-response curves show a parallel shift to the right with no change in the maximum response suggesting a competitive atropine-like action. Such an effect has previously been reported in amphibian tissue but not so far as we can determine in mammalian preparations. 6 The possible mode of action of TEA is discussed.", "contents": "The action of tetraethyl-ammonium chloride on the response of the rat anococcygeus muscle to motor and inhibitory nerve stimulation and to some drugs. 1 Tetraethylammonium chloride (TEA) 0.125 mM to 20 mM potentiates the response of the anococcygeus muscle to field stimulation of the motor adrenergic nerves without affecting the response to noradrenaline suggesting a pre-synaptic origin of potentiation. The potentiation is greatest at low, submaximal, frequencies (2 Hz) of stimulation and only slight at the higher frequency of 20 Hz. This difference is due to the restraint imposed on the demonstration of potentiation by maximal or near maximal motor responses since reduction of the mechanical response at 20 Hz by either phentolamine (post-synaptic block) or guanethidine (pre-synaptic block) resulted in a great increase in potentiation of the response at this frequency. 2 TEA in concentrations up to 1 mM similarly potentites the response to inhibitory nerve stimulation and again the greatest effect is at low frequencies. Higher concentrations (5-20 mM) progressively depress the inhibitory response. It is suggested that TEA may specifically antagonize the post-synaptic action of the inhibitory transmitter and that at higher concentrations of TEA this effect dominates the pre-synaptic action in increasing transmitter release. 3 TEA has no effect on the motor response to tyramine. 4 TEA (5-20 mM) causes a maintained rise in muscle tone. Part of this is abolished by phentolamine but part is resistant. A similar muscle stimulant action of TEA is observed in muscles from rats previously treated with 6-hydroxydopamine in which indirect sympathomimetic drugs and field stimulation could no longer produce a motor response. These results suggest that part of the motor effect of TEA is due to an increased spontaneous release of noradrenaline and part to a direct action on the muscle. 5 TEA 0.125 mM to 20 mM antagonize the stimulant action of carbachol. Dose-response curves show a parallel shift to the right with no change in the maximum response suggesting a competitive atropine-like action. Such an effect has previously been reported in amphibian tissue but not so far as we can determine in mammalian preparations. 6 The possible mode of action of TEA is discussed.", "PMID": 974377}
{"id": "pubmed23n0032_9866", "title": "The kinetics of competitive antagonists on guinea-pig ileum.", "content": "1 The kinetics of action of some competitive muscarinic and histamine antagonists were examined on guinea-pig isolated ileum and their behaviour compared with the predictions of the interaction-limited model described by Paton (1961). 2 The kinetics of antagonism were not consistent with the predictions of this model: (1) The apparent dissociation rate constant calculated from the decrease in occupancy on washout was not independent of the concentration of antagonist. (2) The dissociation rate constant of a 'slow' antagonist calculated from the change in occupancy when a 'fast' antagonist was superimposed varied with the concentration of fast antagonist. (3) If the concentration of slow antagonist was increased when the fast antagonist was superimposed so that the equilibrium occupancy of the 'slow' was the same as before, a transitional phase was observed. 3 The kinetics of antagonism were observed in longitudinal muscle strips and intact pieces of ileum, bathed in Tyrode or Krebs solution, and with isometric and isotonic recording. No evidence was found that the discrepancies between the interaction-limited model and the observed kinetics could be accounted for by the experimental method used. 4 It is therefore concluded that either access is rate-limiting in these circumstances or, if interaction is rate-limiting, some alternative interaction-limited model is required to describe the kinetics of antagonism. In either case it would seem unwise at this time to calculate antagonist-receptor rate constants from the observed kinetics of antagonism.", "contents": "The kinetics of competitive antagonists on guinea-pig ileum. 1 The kinetics of action of some competitive muscarinic and histamine antagonists were examined on guinea-pig isolated ileum and their behaviour compared with the predictions of the interaction-limited model described by Paton (1961). 2 The kinetics of antagonism were not consistent with the predictions of this model: (1) The apparent dissociation rate constant calculated from the decrease in occupancy on washout was not independent of the concentration of antagonist. (2) The dissociation rate constant of a 'slow' antagonist calculated from the change in occupancy when a 'fast' antagonist was superimposed varied with the concentration of fast antagonist. (3) If the concentration of slow antagonist was increased when the fast antagonist was superimposed so that the equilibrium occupancy of the 'slow' was the same as before, a transitional phase was observed. 3 The kinetics of antagonism were observed in longitudinal muscle strips and intact pieces of ileum, bathed in Tyrode or Krebs solution, and with isometric and isotonic recording. No evidence was found that the discrepancies between the interaction-limited model and the observed kinetics could be accounted for by the experimental method used. 4 It is therefore concluded that either access is rate-limiting in these circumstances or, if interaction is rate-limiting, some alternative interaction-limited model is required to describe the kinetics of antagonism. In either case it would seem unwise at this time to calculate antagonist-receptor rate constants from the observed kinetics of antagonism.", "PMID": 974378}
{"id": "pubmed23n0032_9867", "title": "The effect of high rates of vasopressin administration on renal potassium and sodium excretion during potassium loading in the sheep.", "content": "1 The influence of potassium loading on the renal excretion of sodium, potassium and solute during high rate vasopressin administration has been investigated in sheep. 2 Adrenalectomized sheep were infused with 0.43 M KCl at 2 ml/min for 2-2.5 hours. Coincident with the rise in plasma potassium concentration, the urinary excretion of sodium, potassium, solute and water was increased as was the reabsorption of solute-free water. The rates of urinary excretion of sodium and potassium, osmolal clearance (COsm) and solute-free water reabsorption (TcH2O) for the first 50 min of potassium infusion were each found to be linearly related to the plasma potassium concentration. 3 After 50 min an infusion of vasopressin at 1 or 4 mu/min was superimposed on the potassium infusion for a period of 30 minutes. The administration of vasopressin was consistently associated with further augmentation of potassium excretion and clearance, of osmolal clearance and of solute-free water reabsorption to values above those anticipated from the pre-vasopressin regression lines for these parameters. Urinary sodium showed a coincident depression in the rate of excretion and clearance during the same period. 4 Thirty to fifty minutes after the cessation of vasopressin infusion the potassium and sodium excretions had returnied to values which approximated the pre-vasopressin relations between plasma potassium and the urinary excretions of these ions. 5 Both rates of vasopressin infusion were equally effective in increasing the potassium clearance. Any differences in clearance between the two rates of vasopressin administration were not statistically significant. 6 The large increments in potassium excretion (averaging greater than 40%) were interpreted as indicating that, when vasopressin is present at high concentrations, the distal tubule is one site of action of the hormone in the nephron of sheep.", "contents": "The effect of high rates of vasopressin administration on renal potassium and sodium excretion during potassium loading in the sheep. 1 The influence of potassium loading on the renal excretion of sodium, potassium and solute during high rate vasopressin administration has been investigated in sheep. 2 Adrenalectomized sheep were infused with 0.43 M KCl at 2 ml/min for 2-2.5 hours. Coincident with the rise in plasma potassium concentration, the urinary excretion of sodium, potassium, solute and water was increased as was the reabsorption of solute-free water. The rates of urinary excretion of sodium and potassium, osmolal clearance (COsm) and solute-free water reabsorption (TcH2O) for the first 50 min of potassium infusion were each found to be linearly related to the plasma potassium concentration. 3 After 50 min an infusion of vasopressin at 1 or 4 mu/min was superimposed on the potassium infusion for a period of 30 minutes. The administration of vasopressin was consistently associated with further augmentation of potassium excretion and clearance, of osmolal clearance and of solute-free water reabsorption to values above those anticipated from the pre-vasopressin regression lines for these parameters. Urinary sodium showed a coincident depression in the rate of excretion and clearance during the same period. 4 Thirty to fifty minutes after the cessation of vasopressin infusion the potassium and sodium excretions had returnied to values which approximated the pre-vasopressin relations between plasma potassium and the urinary excretions of these ions. 5 Both rates of vasopressin infusion were equally effective in increasing the potassium clearance. Any differences in clearance between the two rates of vasopressin administration were not statistically significant. 6 The large increments in potassium excretion (averaging greater than 40%) were interpreted as indicating that, when vasopressin is present at high concentrations, the distal tubule is one site of action of the hormone in the nephron of sheep.", "PMID": 974379}
{"id": "pubmed23n0032_9868", "title": "The release of a coronary vasodilator metabolite from the guinea-pig isolated perfused heart stimulated by catecholamines, histamine and electrical pacing and by exposure to anoxia.", "content": "1 A procedure involving two guinea-pig isolated hearts perfused in series is described for detecting in the recipient heart the release of a possible coronary vasodilator metabolite from the donor heart. 2 Adrenaline and isoprenaline stimulated the rate and force of contraction and produced a multiphasic coronary vascular response, the predominant phase of which was vasodilatation. When the beta-adrenoceptors of the recipient heart were blocked, stimulation of the donor heart by the catecholamines was associated with a coronary vasodilatation of the recipient heart. 3 Histamine stimulated rate and force of contraction and was predominantly coronary vasodilator. After blockade of histamine H1- and H2-receptors in the recipient heart, coronary vasodilatation followed increases in activity of the donor heart in response to histamine. 4 These vasodilator responses of the recipient heart were attributed to the release from the donor heart of a vasodilator metabolite by the increased activity. This is the proposed mechanism for the predominant coronary vasodilator response to catecholamines and histamine. 5 Periods of electrically-paced tachycardia and anoxia of the donor heart also led to the release of vasodilator activity. 6 The possible identity of the metabolite is discussed.", "contents": "The release of a coronary vasodilator metabolite from the guinea-pig isolated perfused heart stimulated by catecholamines, histamine and electrical pacing and by exposure to anoxia. 1 A procedure involving two guinea-pig isolated hearts perfused in series is described for detecting in the recipient heart the release of a possible coronary vasodilator metabolite from the donor heart. 2 Adrenaline and isoprenaline stimulated the rate and force of contraction and produced a multiphasic coronary vascular response, the predominant phase of which was vasodilatation. When the beta-adrenoceptors of the recipient heart were blocked, stimulation of the donor heart by the catecholamines was associated with a coronary vasodilatation of the recipient heart. 3 Histamine stimulated rate and force of contraction and was predominantly coronary vasodilator. After blockade of histamine H1- and H2-receptors in the recipient heart, coronary vasodilatation followed increases in activity of the donor heart in response to histamine. 4 These vasodilator responses of the recipient heart were attributed to the release from the donor heart of a vasodilator metabolite by the increased activity. This is the proposed mechanism for the predominant coronary vasodilator response to catecholamines and histamine. 5 Periods of electrically-paced tachycardia and anoxia of the donor heart also led to the release of vasodilator activity. 6 The possible identity of the metabolite is discussed.", "PMID": 974380}
{"id": "pubmed23n0032_9869", "title": "Myocardial digoxin uptake: dissociation between digitalis-induced inotropism and myocardial loss of potassium.", "content": "The time course of myocardial uptake of digoxin, of increase in inotropic effect and of changes in myocardial potassium content were studied following a single intravenous dose of digoxin. Nineteen dogs with intact circulation were investigated by the use of a biopsy technique which allowed samplings before and 10, 30, 60, and 90 min after administration of digoxin. The myocardial concentration of digoxin was 196 X 10(-9) mol/kg 10 min after administration of digoxin. Uptake continued at a slower rate, maximum concentration being 293 X 10(-9) mol/kg at 60 minutes. The inotropic effect increased parallel with the uptake of digoxin; 10 min after digoxin, contractility was 127% of the control value and this increased to 139% at 90 minutes. Myocardial potassium content was slightly increased 10 min after digoxin, suggesting an initial stimulation of membrane Na+-K+ ATPase. A subsequent significant fall in the myocardial potassium content probably reflects ATPase inhibition. The temporal dissociation between the early onset of the positive inotropic effect and the delayed inhibition of membrane Na+-K+ ATPase indicates that inotropism of digitalis glycosides is not mediated by the same binding site as that responsible for inhibition of Na+-K+ ATPase.", "contents": "Myocardial digoxin uptake: dissociation between digitalis-induced inotropism and myocardial loss of potassium. The time course of myocardial uptake of digoxin, of increase in inotropic effect and of changes in myocardial potassium content were studied following a single intravenous dose of digoxin. Nineteen dogs with intact circulation were investigated by the use of a biopsy technique which allowed samplings before and 10, 30, 60, and 90 min after administration of digoxin. The myocardial concentration of digoxin was 196 X 10(-9) mol/kg 10 min after administration of digoxin. Uptake continued at a slower rate, maximum concentration being 293 X 10(-9) mol/kg at 60 minutes. The inotropic effect increased parallel with the uptake of digoxin; 10 min after digoxin, contractility was 127% of the control value and this increased to 139% at 90 minutes. Myocardial potassium content was slightly increased 10 min after digoxin, suggesting an initial stimulation of membrane Na+-K+ ATPase. A subsequent significant fall in the myocardial potassium content probably reflects ATPase inhibition. The temporal dissociation between the early onset of the positive inotropic effect and the delayed inhibition of membrane Na+-K+ ATPase indicates that inotropism of digitalis glycosides is not mediated by the same binding site as that responsible for inhibition of Na+-K+ ATPase.", "PMID": 974381}
{"id": "pubmed23n0032_9870", "title": "On the decrease of myocardial oxygen consumption induced by chloracyzine.", "content": "The effects of chloracyzine (2-chloro-10-(3-diethylaminopropionyl)-phenothiazine hydrochloride; antianginal drug) on myocardial oxygen consumption were studied in open-chest cats and cat isolated hearts using oximetry and polarography respectively. It was found that chloracyzine produced a decrease in myocardial oxygen consumption accompanied by a reduction in coronary blood flow preceded by transient coronary dilatation. Chloracyzine produced an insignificant increase in arterial pressure; heart rate increased slightly in the open-chest experiments but not in the isolated heart. It is suggested that reduced oxygen uptake after chloracyzine is realized through improved efficiency in the use of oxygen.", "contents": "On the decrease of myocardial oxygen consumption induced by chloracyzine. The effects of chloracyzine (2-chloro-10-(3-diethylaminopropionyl)-phenothiazine hydrochloride; antianginal drug) on myocardial oxygen consumption were studied in open-chest cats and cat isolated hearts using oximetry and polarography respectively. It was found that chloracyzine produced a decrease in myocardial oxygen consumption accompanied by a reduction in coronary blood flow preceded by transient coronary dilatation. Chloracyzine produced an insignificant increase in arterial pressure; heart rate increased slightly in the open-chest experiments but not in the isolated heart. It is suggested that reduced oxygen uptake after chloracyzine is realized through improved efficiency in the use of oxygen.", "PMID": 974382}
{"id": "pubmed23n0032_9871", "title": "Bradykinin and functional vasodilatation in the salivary gland.", "content": "Blood flow through the submandibular gland of the dog was measured and the venous effluent monitored for potential mediators of the functional vasodilatation by passing it over a series of assay tissues. On chorda lingual nerve stimulation there was salivation, an increased blood flow and the release of a bradykinin-like substance (kinin) into the venous effluent. In about half of the preparations, increasing the frequency of stimulation from 2 to 10 Hz led to an increased output of kinin, whereas in the others successive stimulations led to a decreasing output of kinin in the face of normal secretory and vascular responses. Following the administration of atropine, the vasodilatation persisted, salivation was abolished and after several stimulations kinin release could no longer be detected. Release of prostaglandin did not appear to be responsible for the vasodilatation. It is concluded that neither kallikrein nor kinin is the main mediator of parasympathetic vasodilatation in the salivary gland.", "contents": "Bradykinin and functional vasodilatation in the salivary gland. Blood flow through the submandibular gland of the dog was measured and the venous effluent monitored for potential mediators of the functional vasodilatation by passing it over a series of assay tissues. On chorda lingual nerve stimulation there was salivation, an increased blood flow and the release of a bradykinin-like substance (kinin) into the venous effluent. In about half of the preparations, increasing the frequency of stimulation from 2 to 10 Hz led to an increased output of kinin, whereas in the others successive stimulations led to a decreasing output of kinin in the face of normal secretory and vascular responses. Following the administration of atropine, the vasodilatation persisted, salivation was abolished and after several stimulations kinin release could no longer be detected. Release of prostaglandin did not appear to be responsible for the vasodilatation. It is concluded that neither kallikrein nor kinin is the main mediator of parasympathetic vasodilatation in the salivary gland.", "PMID": 974383}
{"id": "pubmed23n0032_9872", "title": "Effect of indomethacin and prostaglandin on the smooth muscle contracting activity of angiotensin and other agonists.", "content": "Indomethacin had an equal inhibitory effect on the response of the guinea-pig isolated ileum to angiotensin II (angiotensin), bradykinin, histamine and acetylcholine. This effect did not seem to result from inhibition of prostaglandin synthesis, as it did not depend on the time of treatment with indomethacin. Prostaglandin E2 (prostaglandin) potentiated the responses of the guinea-pig ileum to angiotensin, bradykinin, histamine and acetylcholine without significant differences in the effects observed. In the rabbit isolated mesenteric and coeliac arteries, indomethacin had an equal potentiating effect on the responses to angiotensin and to adrenaline. In these organs pre-incubation with indomethacin was necessary for the effect to be observed, and this effect lasted for 2 h or more after that drug was removed from the medium. No cross-tachyphylaxis between angiotensin and adrenaline was observed in the rabbit mesenteric and coeliac arteries. It is concluded that the effects of indomethacin and prostaglandin on the response of the guinea-pig ileum to the four agonists result from an action on the smooth muscle contractile mechanism per se rather than from an inhibitory action on the release of endogenous prostaglandin produced by the four agonists. The results with the rabbit isolated arteries indicate that tachyphylaxis to angiotensin in these organs is not caused by prostaglandin release.", "contents": "Effect of indomethacin and prostaglandin on the smooth muscle contracting activity of angiotensin and other agonists. Indomethacin had an equal inhibitory effect on the response of the guinea-pig isolated ileum to angiotensin II (angiotensin), bradykinin, histamine and acetylcholine. This effect did not seem to result from inhibition of prostaglandin synthesis, as it did not depend on the time of treatment with indomethacin. Prostaglandin E2 (prostaglandin) potentiated the responses of the guinea-pig ileum to angiotensin, bradykinin, histamine and acetylcholine without significant differences in the effects observed. In the rabbit isolated mesenteric and coeliac arteries, indomethacin had an equal potentiating effect on the responses to angiotensin and to adrenaline. In these organs pre-incubation with indomethacin was necessary for the effect to be observed, and this effect lasted for 2 h or more after that drug was removed from the medium. No cross-tachyphylaxis between angiotensin and adrenaline was observed in the rabbit mesenteric and coeliac arteries. It is concluded that the effects of indomethacin and prostaglandin on the response of the guinea-pig ileum to the four agonists result from an action on the smooth muscle contractile mechanism per se rather than from an inhibitory action on the release of endogenous prostaglandin produced by the four agonists. The results with the rabbit isolated arteries indicate that tachyphylaxis to angiotensin in these organs is not caused by prostaglandin release.", "PMID": 974384}
{"id": "pubmed23n0032_9873", "title": "The denervated cremaster muscle of the guinea-pig as a pharmacological preparation.", "content": "The pharmacological responses of both denervated and innervated cremaster muscle preparations from the guinea-pig have been investigated and compared. Acetylcholine (ACh) dose-response curves were obtained in both preparations. The affinity constants for (+)-tubocurarine and for atropine were calculated and indicated that the ACh receptors in both preparations were nicotinic. Histamine dose-response curves could be obtained only in the denervated preparation. The response was unaltered by metiamide, and the affinity constant for mepyramine fitted in with those previously obtained by others on ileum and trachea, indicating that the histamine receptors are H1 in type. Sustained contractions were obtained to adrenaline and noradrenaline but not isoprenaline, with the denervated preparations. Schultz-Dale responses were obtained with denervated muscle in sensitized guinea-pigs. This preparation did not respond to 5-hydroxytryptamine, bradykinin, or the slow-reacting substance of anaphylaxis.", "contents": "The denervated cremaster muscle of the guinea-pig as a pharmacological preparation. The pharmacological responses of both denervated and innervated cremaster muscle preparations from the guinea-pig have been investigated and compared. Acetylcholine (ACh) dose-response curves were obtained in both preparations. The affinity constants for (+)-tubocurarine and for atropine were calculated and indicated that the ACh receptors in both preparations were nicotinic. Histamine dose-response curves could be obtained only in the denervated preparation. The response was unaltered by metiamide, and the affinity constant for mepyramine fitted in with those previously obtained by others on ileum and trachea, indicating that the histamine receptors are H1 in type. Sustained contractions were obtained to adrenaline and noradrenaline but not isoprenaline, with the denervated preparations. Schultz-Dale responses were obtained with denervated muscle in sensitized guinea-pigs. This preparation did not respond to 5-hydroxytryptamine, bradykinin, or the slow-reacting substance of anaphylaxis.", "PMID": 974385}
{"id": "pubmed23n0032_9874", "title": "The action of microelectrophoretically applied L-3,4-dihydroxyphenylalanine (DOPA) on single cortical neurones.", "content": "The technique of microelectrophoresis was used in order to compare the actions of L-3,4-dihydroxyphenylalanine (DOPA) and noradrenaline on single neurones in the cerebral cortices of cats and rats. DOPA could both excite and depress cortical neurones. Cells excited by DOPA were also excited by noradrenaline and cells depressed by DOPA were also depressed by noradrenaline. In the case of both excitatory and depressant responses, DOPA appeared to be less potent than noradrenaline. Responses to DOPA and noradrenaline could be antagonized by phentolamine and propranolol. Responses to acetylcholine were not affected. Responses to acetylcholine, but not responses to DOPA, were antagonized by atropine. The results indicate that locally applied DOPA may mimic the actions of noradrenaline on cortical neurones. Possible mechanisms for these effects of DOPA are discussed.", "contents": "The action of microelectrophoretically applied L-3,4-dihydroxyphenylalanine (DOPA) on single cortical neurones. The technique of microelectrophoresis was used in order to compare the actions of L-3,4-dihydroxyphenylalanine (DOPA) and noradrenaline on single neurones in the cerebral cortices of cats and rats. DOPA could both excite and depress cortical neurones. Cells excited by DOPA were also excited by noradrenaline and cells depressed by DOPA were also depressed by noradrenaline. In the case of both excitatory and depressant responses, DOPA appeared to be less potent than noradrenaline. Responses to DOPA and noradrenaline could be antagonized by phentolamine and propranolol. Responses to acetylcholine were not affected. Responses to acetylcholine, but not responses to DOPA, were antagonized by atropine. The results indicate that locally applied DOPA may mimic the actions of noradrenaline on cortical neurones. Possible mechanisms for these effects of DOPA are discussed.", "PMID": 974386}
{"id": "pubmed23n0032_9875", "title": "Effects of ketamine on the peripheral autonomic nervous system of the rat.", "content": "The effects of ketamine (2-(o-chlorophenyl) 2-methylaminocyclohexanone) (2-50 mg/kg) on the responses of the pithed rat arterial pressure, anococcygeus muscle and colon to selective stimulation of the spinal autonomic outflows were examined. Ketamine depressed the vasopressor response produced by stimulation of the lumbar sympathetic outflow in a dose-dependent manner but did not significantly affect the pressor response to intravenous noradrenaline (NA) administration. Ketamine depressed the motor responses of the anococcygeus to stimulation of the pre-ganglionic lumbar sympathetic outflow or to stimulation of post-ganglionic fibres in the sacral region in a dose-dependent manner, the response to preganglionic stimulation being relatively more sensitive to such depression. The anococcygeus response to NA was significantly potentiated with doses of ketamine of 20 mg/kg and 50 mg/kg. Ketamine depressed the motor response of the smooth muscle of the colon to stimulation of the sacral parasympathetic outflow in a dose-dependent manner and at lower doses than were required to produce an equivalent depression of the sympathetic responses in the other tissues. A comparison was made of the effects of ketamine and cocaine on the motor responses of the anococcygeus muscle in vitro to NA, carbachol and field stimulation. Both ketamine and cocaine produced a non-specific depression of all responses at high doses whereas cocaine but not ketamine produced a large potentiation of NA and motor nerve responses at lower doses. The results are discussed in relation to the hypothesis that ketamine might elevate blood pressure in conscious animals and man by potentiating vascular adrenergic responses.", "contents": "Effects of ketamine on the peripheral autonomic nervous system of the rat. The effects of ketamine (2-(o-chlorophenyl) 2-methylaminocyclohexanone) (2-50 mg/kg) on the responses of the pithed rat arterial pressure, anococcygeus muscle and colon to selective stimulation of the spinal autonomic outflows were examined. Ketamine depressed the vasopressor response produced by stimulation of the lumbar sympathetic outflow in a dose-dependent manner but did not significantly affect the pressor response to intravenous noradrenaline (NA) administration. Ketamine depressed the motor responses of the anococcygeus to stimulation of the pre-ganglionic lumbar sympathetic outflow or to stimulation of post-ganglionic fibres in the sacral region in a dose-dependent manner, the response to preganglionic stimulation being relatively more sensitive to such depression. The anococcygeus response to NA was significantly potentiated with doses of ketamine of 20 mg/kg and 50 mg/kg. Ketamine depressed the motor response of the smooth muscle of the colon to stimulation of the sacral parasympathetic outflow in a dose-dependent manner and at lower doses than were required to produce an equivalent depression of the sympathetic responses in the other tissues. A comparison was made of the effects of ketamine and cocaine on the motor responses of the anococcygeus muscle in vitro to NA, carbachol and field stimulation. Both ketamine and cocaine produced a non-specific depression of all responses at high doses whereas cocaine but not ketamine produced a large potentiation of NA and motor nerve responses at lower doses. The results are discussed in relation to the hypothesis that ketamine might elevate blood pressure in conscious animals and man by potentiating vascular adrenergic responses.", "PMID": 974387}
{"id": "pubmed23n0032_9876", "title": "Renal function and renal venous prostaglandin concentrations during different stages of experimental renal hypertension in the rat.", "content": "Renal hypertension was produced in rats and the changes in renal function, renal venous prostaglandin E2 and F2alpha concentrations and secretion rates were studied at various times. Renal plasma flow transiently fell in the ischaemic kidney 2 weeks after clamping, whilst that of the other kidney did not change. Glomerular filtration rate remained constant in both kidneys throughout the entire study. Prostaglandins E2 and F2alpha concentrations rose in the venous plasma from the ischaemic kidney, but did not change in the other kidney and appeared to be inversely related to renal plasma flow. Calculated secretion rate of both prostaglandins fell in the ischaemic kidney and did not change in the other kidney. Clamping the second kidney, two weeks after the first, caused a further elevation in blood pressure, a fall in renal plasma flow and a fall in prostaglandin secretion rate in both kidneys. The implications of these prostaglandin changes are discussed.", "contents": "Renal function and renal venous prostaglandin concentrations during different stages of experimental renal hypertension in the rat. Renal hypertension was produced in rats and the changes in renal function, renal venous prostaglandin E2 and F2alpha concentrations and secretion rates were studied at various times. Renal plasma flow transiently fell in the ischaemic kidney 2 weeks after clamping, whilst that of the other kidney did not change. Glomerular filtration rate remained constant in both kidneys throughout the entire study. Prostaglandins E2 and F2alpha concentrations rose in the venous plasma from the ischaemic kidney, but did not change in the other kidney and appeared to be inversely related to renal plasma flow. Calculated secretion rate of both prostaglandins fell in the ischaemic kidney and did not change in the other kidney. Clamping the second kidney, two weeks after the first, caused a further elevation in blood pressure, a fall in renal plasma flow and a fall in prostaglandin secretion rate in both kidneys. The implications of these prostaglandin changes are discussed.", "PMID": 974388}
{"id": "pubmed23n0032_9877", "title": "The lack of effect of oxytetracycline on responses to sympathetic nerve stimulation and catecholamines in vascular tissue.", "content": "The effects of oxytetracycline, an inhibitor of amine binding in connective tissue, on the responses of perfused rabbit ear arteries to sympathetic nerve stimulation and to intraluminally administered noradrenaline were examined. The contractions of aortic strips to catecholamines in the presence of oxytetracycline were also examined. Oxytetracycline (0.1 mM) had no discernable effect on the magnitude of constrictions, measured as reductions in flow, produced by either nerve stimulation (0.5-10 Hz) or noradrenaline (0.5-50 ng) in the ear artery. In addition, the time taken for vessels to recover towards control flow values after endogenously released or exogenously applied noradrenaline had acted was not increased by oxytetracycline. Oxytetracycline (0.1 mM) did not alter the position or shape of the concentration-response curve to noradrenaline nor did it enhance the amplitude of individual responses to catecholamines in aortic strips. It is concluded, contrary to the observations of Powis (1973), that oxytetracycline does not increase the magnitude or duration of responses to sympathetic nerve activation or to catecholamines and that binding to connective tissue is of no material consequence in terminating their action in vascular tissue.", "contents": "The lack of effect of oxytetracycline on responses to sympathetic nerve stimulation and catecholamines in vascular tissue. The effects of oxytetracycline, an inhibitor of amine binding in connective tissue, on the responses of perfused rabbit ear arteries to sympathetic nerve stimulation and to intraluminally administered noradrenaline were examined. The contractions of aortic strips to catecholamines in the presence of oxytetracycline were also examined. Oxytetracycline (0.1 mM) had no discernable effect on the magnitude of constrictions, measured as reductions in flow, produced by either nerve stimulation (0.5-10 Hz) or noradrenaline (0.5-50 ng) in the ear artery. In addition, the time taken for vessels to recover towards control flow values after endogenously released or exogenously applied noradrenaline had acted was not increased by oxytetracycline. Oxytetracycline (0.1 mM) did not alter the position or shape of the concentration-response curve to noradrenaline nor did it enhance the amplitude of individual responses to catecholamines in aortic strips. It is concluded, contrary to the observations of Powis (1973), that oxytetracycline does not increase the magnitude or duration of responses to sympathetic nerve activation or to catecholamines and that binding to connective tissue is of no material consequence in terminating their action in vascular tissue.", "PMID": 974389}
{"id": "pubmed23n0032_9878", "title": "Study of disease associations from linked records.", "content": "Comparisons are made between the principal diagnosis on discharge from hospital in successive periods of inpatient care for persons in the Oxford Record Linkage Study area admitted over a period of years. The observed numbers of pairs of diagnoses are compared with expected numbers computed to take account of the discharge rates in the population by age and sex and the number of man years of exposure. Three topics have been selected to display some of the types of analysis possible with the very extensive material: mental disorder and diseases of the central nervous system, hospital discharges preceding those for neoplasm, and discharges following those for tuberculosis. The details of the method of calculation of the observed and expected numbers and the assumptions and approximations involved are given in an Appendix.", "contents": "Study of disease associations from linked records. Comparisons are made between the principal diagnosis on discharge from hospital in successive periods of inpatient care for persons in the Oxford Record Linkage Study area admitted over a period of years. The observed numbers of pairs of diagnoses are compared with expected numbers computed to take account of the discharge rates in the population by age and sex and the number of man years of exposure. Three topics have been selected to display some of the types of analysis possible with the very extensive material: mental disorder and diseases of the central nervous system, hospital discharges preceding those for neoplasm, and discharges following those for tuberculosis. The details of the method of calculation of the observed and expected numbers and the assumptions and approximations involved are given in an Appendix.", "PMID": 974433}
{"id": "pubmed23n0032_9879", "title": "Cancer mortality and saccharin consumption in diabetics.", "content": "The mortality experience of 5971 members of the British Diabetic Association (BDA) was followed-up for between five and eight years to mid-1973. Overall, 1207 deaths occurred compared with 778 expected from the mortality of the population of England and Wales in 1972. This excess of deaths was due almost entirely to diabetes mellitus and ischaemic heart disease. Deaths from cancer (128) were significantly fewer than expected (168), mainly because of a deficit in the number of deaths from cancers related to smoking (cancers of the buccal cavity and pharynx, oesophagus, respiratory system, and bladder). There was also a lower than expected mortality from chronic bronchitis and emphysema. Data on saccharin consumption by BDA members showed that more than half of them used saccharin tablets daily, with an overall daily intake of three to six tablets, depending on age and sex. Information on a small sample of survivors from the mortality study suggested that about 23% of them would have taken saccharin daily for 10 years or more and 10% for 25 years or more by the end of the follow-up. It was concluded that these relatively high levels of saccharin intake had not increased the risk of cancer in general among BDA members.", "contents": "Cancer mortality and saccharin consumption in diabetics. The mortality experience of 5971 members of the British Diabetic Association (BDA) was followed-up for between five and eight years to mid-1973. Overall, 1207 deaths occurred compared with 778 expected from the mortality of the population of England and Wales in 1972. This excess of deaths was due almost entirely to diabetes mellitus and ischaemic heart disease. Deaths from cancer (128) were significantly fewer than expected (168), mainly because of a deficit in the number of deaths from cancers related to smoking (cancers of the buccal cavity and pharynx, oesophagus, respiratory system, and bladder). There was also a lower than expected mortality from chronic bronchitis and emphysema. Data on saccharin consumption by BDA members showed that more than half of them used saccharin tablets daily, with an overall daily intake of three to six tablets, depending on age and sex. Information on a small sample of survivors from the mortality study suggested that about 23% of them would have taken saccharin daily for 10 years or more and 10% for 25 years or more by the end of the follow-up. It was concluded that these relatively high levels of saccharin intake had not increased the risk of cancer in general among BDA members.", "PMID": 974434}
{"id": "pubmed23n0032_9880", "title": "The relationship between blood pressure and biochemical risk factors in a general population.", "content": "The relationship between blood pressure, ponderal index, sex, blood glucose, haemoglobin, serum uric acid, calcium cholesterol and creatinine, and albumin has been examined in 698 subjects aged between 44 and 49 years from the register of a group general practice. Sixty per cent of the variation in systolic pressure could be explained by statistically significant associations with diastolic pressure, sex, blood glucose, serum calcium, and cholesterol. The diastolic blood pressure (not corrected for systolic pressure) was significantly related only to ponderal index, haemoglobin in men, and cholesterol in women. Pulse pressure was also positively related to the risk factors blood glucose, serum cholesterol, and calcium. The possibility is discussed that one or more of these variables reduce aortic compliance and that the serum calcium contributes to this end. Diastolic, but not systolic pressure, had a prime association with relative weight, obesity being only basically associated with an increase in diastolic pressure.", "contents": "The relationship between blood pressure and biochemical risk factors in a general population. The relationship between blood pressure, ponderal index, sex, blood glucose, haemoglobin, serum uric acid, calcium cholesterol and creatinine, and albumin has been examined in 698 subjects aged between 44 and 49 years from the register of a group general practice. Sixty per cent of the variation in systolic pressure could be explained by statistically significant associations with diastolic pressure, sex, blood glucose, serum calcium, and cholesterol. The diastolic blood pressure (not corrected for systolic pressure) was significantly related only to ponderal index, haemoglobin in men, and cholesterol in women. Pulse pressure was also positively related to the risk factors blood glucose, serum cholesterol, and calcium. The possibility is discussed that one or more of these variables reduce aortic compliance and that the serum calcium contributes to this end. Diastolic, but not systolic pressure, had a prime association with relative weight, obesity being only basically associated with an increase in diastolic pressure.", "PMID": 974435}
{"id": "pubmed23n0032_9881", "title": "Mental capability of children exposed to lead pollution.", "content": "'Eleven-plus' school examination scores were obtained for 851 Birmingham children residing since birth in a lead-polluted area and 1642 children residing in two similar but unpolluted areas. It was found that the children in the lead-polluted area actually scored higher on the average than children in the control areas. Within the area of lead contamination, children living closest to the source of pollution did not have significantly lower scores than children living further away. The results indicate that lead pollution of the magnitude reported in this investigation did not have a demonstrable effect on the mental capabilities of children in the affected community.", "contents": "Mental capability of children exposed to lead pollution. 'Eleven-plus' school examination scores were obtained for 851 Birmingham children residing since birth in a lead-polluted area and 1642 children residing in two similar but unpolluted areas. It was found that the children in the lead-polluted area actually scored higher on the average than children in the control areas. Within the area of lead contamination, children living closest to the source of pollution did not have significantly lower scores than children living further away. The results indicate that lead pollution of the magnitude reported in this investigation did not have a demonstrable effect on the mental capabilities of children in the affected community.", "PMID": 974436}
{"id": "pubmed23n0032_9882", "title": "A study of the validity of the Hospital Activity Analysis information.", "content": "For a number of years the medical profession and its administrators have been using statistical tabulations from the Hospital Activity Analysis returns. The quality of this information has often been criticized and no attempt has been made to quantify the levels of 'errors' in England and Wales. This paper reports the findings of such a study in Nottingham, and concludes that the Hospital Activity Analysis system in that area is almost as good as the clinical notes from which it is derived.", "contents": "A study of the validity of the Hospital Activity Analysis information. For a number of years the medical profession and its administrators have been using statistical tabulations from the Hospital Activity Analysis returns. The quality of this information has often been criticized and no attempt has been made to quantify the levels of 'errors' in England and Wales. This paper reports the findings of such a study in Nottingham, and concludes that the Hospital Activity Analysis system in that area is almost as good as the clinical notes from which it is derived.", "PMID": 974438}
{"id": "pubmed23n0032_9883", "title": "Epidemiology of twin births from a climatic point of view.", "content": "The twinning rate was analysed using figures taken from statistics for Japan. During the years 1955 to 1959 there were 58 570 twin deliveries out of a total of 9 088 233, a frequency of 6-44 per 1000. Using Winberg's differential method for zygosity estimation, the monozygotic twinning rate was 4-04 per 1000 and the dizygotic was 2-40. Twinning rates in 46 prefectures were also estimated and their correlations with meteorological parameters as well as with the mortality from cerebrovascular disease were examined. The twinning rates increased from a low level in the south west to a high level in the north east of Japan. The tendency was more obvious for the dizygotic than the monozygotic twinning rate. A negative correlation was observed between incidence rates of dizygotic twins and mean air-temperatures throughout the year, and a positive one between incidences of dizygotic twins and mortality rates for vascular lesions affecting the central nervous system in 46 prefectures. A seasonal variation of twinning rates was also observed in Niigata City for statistics of births during the periods 1948-55 and 1963-70. This took the form of a bimodal curve, with high conception rates for twin births in intermediate mild seasons, the spring and autumn and, low rates in the more stressful hot and cold seasons. These relationships between climate and twin births within a single race-group are discussed.", "contents": "Epidemiology of twin births from a climatic point of view. The twinning rate was analysed using figures taken from statistics for Japan. During the years 1955 to 1959 there were 58 570 twin deliveries out of a total of 9 088 233, a frequency of 6-44 per 1000. Using Winberg's differential method for zygosity estimation, the monozygotic twinning rate was 4-04 per 1000 and the dizygotic was 2-40. Twinning rates in 46 prefectures were also estimated and their correlations with meteorological parameters as well as with the mortality from cerebrovascular disease were examined. The twinning rates increased from a low level in the south west to a high level in the north east of Japan. The tendency was more obvious for the dizygotic than the monozygotic twinning rate. A negative correlation was observed between incidence rates of dizygotic twins and mean air-temperatures throughout the year, and a positive one between incidences of dizygotic twins and mortality rates for vascular lesions affecting the central nervous system in 46 prefectures. A seasonal variation of twinning rates was also observed in Niigata City for statistics of births during the periods 1948-55 and 1963-70. This took the form of a bimodal curve, with high conception rates for twin births in intermediate mild seasons, the spring and autumn and, low rates in the more stressful hot and cold seasons. These relationships between climate and twin births within a single race-group are discussed.", "PMID": 974437}
{"id": "pubmed23n0032_9884", "title": "Accuracy of cancer registration.", "content": "In South Wales cancer registration is done principally by means of the Hospital Activity Analysis. Altogether 1460 hospital records of cancer patients (19% of the 1972 registrations received by May 1973) were studied and the principal items of information required for cancer registrations by the Office of Population Censuses and Surveys were copied and subsequently compared with the corresponding registrations at the Welsh Hospital Board's cancer bureau. Differences between these 're-registrations' and the original registrations were analysed item by item. There were 234 registrations with errors in the diagnostic summary (although 110 of these would cause misclassification only under the fourth digit of the ICD code), 164 with errors in date of birth (36 of which would cause classification in the wrong WHO age group) and 198 with errors in the date of registration (112 of which were wrongly ascribed to the year 1972). Error and omission rates were particularly high for NHS number, occupation, place of birth, and histology.", "contents": "Accuracy of cancer registration. In South Wales cancer registration is done principally by means of the Hospital Activity Analysis. Altogether 1460 hospital records of cancer patients (19% of the 1972 registrations received by May 1973) were studied and the principal items of information required for cancer registrations by the Office of Population Censuses and Surveys were copied and subsequently compared with the corresponding registrations at the Welsh Hospital Board's cancer bureau. Differences between these 're-registrations' and the original registrations were analysed item by item. There were 234 registrations with errors in the diagnostic summary (although 110 of these would cause misclassification only under the fourth digit of the ICD code), 164 with errors in date of birth (36 of which would cause classification in the wrong WHO age group) and 198 with errors in the date of registration (112 of which were wrongly ascribed to the year 1972). Error and omission rates were particularly high for NHS number, occupation, place of birth, and histology.", "PMID": 974439}
{"id": "pubmed23n0032_9885", "title": "Personality and symptom pattern in depression.", "content": "This paper reports relationships between symptoms and premorbid personality in a varied sample of depressed patients. Symptoms were rated by a psychiatrist at clinical interview; personality was rated by patients on a self-report after clinical improvement, using the Maudsley Personality Inventory and an inventory of obsessive, hysterical and oral personality. Additional ratings on the latter were obtained at interview with a relative. The most prominent finding was that patients with premorbid neuroticism also showed a neurotic rather than an endogenous symptom pattern. Additional relationships were relatively weak but consistent with previous studies. Depressives with neurotic rather than endogenous symptom pattern showed more evidence of oral dependent personality and less obsessionality. Patients with hysterical personalities tended to be less severely ill and to show a pattern characterized by mixed depression and hostility with less evidence of anxiety.", "contents": "Personality and symptom pattern in depression. This paper reports relationships between symptoms and premorbid personality in a varied sample of depressed patients. Symptoms were rated by a psychiatrist at clinical interview; personality was rated by patients on a self-report after clinical improvement, using the Maudsley Personality Inventory and an inventory of obsessive, hysterical and oral personality. Additional ratings on the latter were obtained at interview with a relative. The most prominent finding was that patients with premorbid neuroticism also showed a neurotic rather than an endogenous symptom pattern. Additional relationships were relatively weak but consistent with previous studies. Depressives with neurotic rather than endogenous symptom pattern showed more evidence of oral dependent personality and less obsessionality. Patients with hysterical personalities tended to be less severely ill and to show a pattern characterized by mixed depression and hostility with less evidence of anxiety.", "PMID": 974440}
{"id": "pubmed23n0032_9886", "title": "Cardiac effects of different tricyclic antidepressant drugs.", "content": "The effects of tricyclic antidepressants on the heart are reviewed. Statistically significant increases in heart rate and in atrioventricular conduction times were found following the administration of tricyclic drugs to 32 depressed patients. The method of His bundle electrocardiography was used to study atrioventricular conduction in ambulant patients and in patients admitted after an overdose of tricyclic antidepressant drugs. Distal conduction defects were frequently found in patients following tricyclic overdosage, but these were not seen with doxepin overdosage. Impaired distal conduction was also found in occasional patients on therapeutic doses of tricyclic drugs. Some animal experiments giving similar results to the above clinical findings are also described. The clinical implications of these findings are discussed.", "contents": "Cardiac effects of different tricyclic antidepressant drugs. The effects of tricyclic antidepressants on the heart are reviewed. Statistically significant increases in heart rate and in atrioventricular conduction times were found following the administration of tricyclic drugs to 32 depressed patients. The method of His bundle electrocardiography was used to study atrioventricular conduction in ambulant patients and in patients admitted after an overdose of tricyclic antidepressant drugs. Distal conduction defects were frequently found in patients following tricyclic overdosage, but these were not seen with doxepin overdosage. Impaired distal conduction was also found in occasional patients on therapeutic doses of tricyclic drugs. Some animal experiments giving similar results to the above clinical findings are also described. The clinical implications of these findings are discussed.", "PMID": 974441}
{"id": "pubmed23n0032_9887", "title": "Mianserin hydrochloride: a novel antidepressant.", "content": "The antidepressant activities of mianserin hydrochloride were investigated and compared with those of amitriptyline. The therapeutic efficacy of the two drugs appeared similar, but the incidence of side-effects was significantly higher with amitriptyline. Plasma levels of mianserin were determined during the trial and were not related to the therapeutic activity of the drug.", "contents": "Mianserin hydrochloride: a novel antidepressant. The antidepressant activities of mianserin hydrochloride were investigated and compared with those of amitriptyline. The therapeutic efficacy of the two drugs appeared similar, but the incidence of side-effects was significantly higher with amitriptyline. Plasma levels of mianserin were determined during the trial and were not related to the therapeutic activity of the drug.", "PMID": 974442}
{"id": "pubmed23n0032_9888", "title": "Circadian body temperature in chronic schizophrenia.", "content": "The 24-hour oral temperatures of 51 chronic schizophrenic patients, 19 non-schizophrenic chronic psychiatric patients and 14 non-patients have been measured and compared. The chronic schizophrenias have an ealier peak temperature and differ significantly from the other groups. Compared with the non-schizophrenic patients their hourly mean temperatures reach significantly higher levels in the morning. Compared with the non-patients their mean temperatures fall to significantly lower levels in the evening after 5:00 pm.", "contents": "Circadian body temperature in chronic schizophrenia. The 24-hour oral temperatures of 51 chronic schizophrenic patients, 19 non-schizophrenic chronic psychiatric patients and 14 non-patients have been measured and compared. The chronic schizophrenias have an ealier peak temperature and differ significantly from the other groups. Compared with the non-schizophrenic patients their hourly mean temperatures reach significantly higher levels in the morning. Compared with the non-patients their mean temperatures fall to significantly lower levels in the evening after 5:00 pm.", "PMID": 974443}
{"id": "pubmed23n0032_9889", "title": "Mental disorder and season of birth--a southern hemisphere study.", "content": "Studies on the relationship between season of birth and mental disorder have been substantially confined to northern hemisphere regions. Such studies have generally found an excess of winter births of schizophrenics, and variably an excess of winter births of manic-depressive and mentally retarded patients. In the present study information on sex, diagnosis and date of birth was obtained on all 20,358 patients first admitted to psychiatric facilities in New South Wales between July 1970 and June 1974 and born in New South Wales. The collective 1962-71 monthly live-births for New South Wales were used as a control. A significant winter excess was found for the female schizophrenic group, while a significant spring excess was found for neurotic patients, most marked in those with anxiety neurosis. It is hypothesized that the relationship between schizophrenia and winter birth is consequent upon a greater sensitivity of schizophrenics to those physiological factors which determine conception in the general population.", "contents": "Mental disorder and season of birth--a southern hemisphere study. Studies on the relationship between season of birth and mental disorder have been substantially confined to northern hemisphere regions. Such studies have generally found an excess of winter births of schizophrenics, and variably an excess of winter births of manic-depressive and mentally retarded patients. In the present study information on sex, diagnosis and date of birth was obtained on all 20,358 patients first admitted to psychiatric facilities in New South Wales between July 1970 and June 1974 and born in New South Wales. The collective 1962-71 monthly live-births for New South Wales were used as a control. A significant winter excess was found for the female schizophrenic group, while a significant spring excess was found for neurotic patients, most marked in those with anxiety neurosis. It is hypothesized that the relationship between schizophrenia and winter birth is consequent upon a greater sensitivity of schizophrenics to those physiological factors which determine conception in the general population.", "PMID": 974444}
{"id": "pubmed23n0032_9890", "title": "Predicting success in the treatment of psychopaths.", "content": "Factors from the social history of male psychopaths were examined in relation to their success or failure as measured by re-convictions of psychiatric hospital re-admissions 2-3 years after treatment at Henderson Hospital. From these data a weighted prediction formula was calculated and was tested out on a further cohort of patients. As a prediction instrument it was found to be reliable to a degree of significance just above the 1 per cent level. Other aspects of the subsequent career of treated patients are noted, in particular a tendency to early but often short-lived relapse and then longer standing success.", "contents": "Predicting success in the treatment of psychopaths. Factors from the social history of male psychopaths were examined in relation to their success or failure as measured by re-convictions of psychiatric hospital re-admissions 2-3 years after treatment at Henderson Hospital. From these data a weighted prediction formula was calculated and was tested out on a further cohort of patients. As a prediction instrument it was found to be reliable to a degree of significance just above the 1 per cent level. Other aspects of the subsequent career of treated patients are noted, in particular a tendency to early but often short-lived relapse and then longer standing success.", "PMID": 974445}
{"id": "pubmed23n0032_9891", "title": "The effects of stimulus attributes upon latency of word recognition.", "content": "This experiment investigated the properties affecting the time taken to read individual words and to discriminate between words and non-words. The number of letters in a word was found to affect the time taken to read it aloud, but not the time taken to discriminate it from non-words. The frequency of a word affected the time for discrimination, but not that for reading. Non-words which obeyed the rules of English orthography and phonology were more difficult to discriminate from words than those which violated those rules. Imageability, concreteness, and the number of syllables in a word were found not to affect performance, nor were derived nouns more difficult to process than simple nouns. It is suggested that reading aloud employs grapheme-phoneme translation based upon a letter-by-letter analysis of the stimulus: that discriminating words from non-words obeying the rules of English orthography and phonology employs a search of the lexicon based upon a holistic analysis of the stimulus; and that discriminating words from non-words violating those rules employs a direct test of the regularity of the stimulus based upon the combinatory rules of English orthography.", "contents": "The effects of stimulus attributes upon latency of word recognition. This experiment investigated the properties affecting the time taken to read individual words and to discriminate between words and non-words. The number of letters in a word was found to affect the time taken to read it aloud, but not the time taken to discriminate it from non-words. The frequency of a word affected the time for discrimination, but not that for reading. Non-words which obeyed the rules of English orthography and phonology were more difficult to discriminate from words than those which violated those rules. Imageability, concreteness, and the number of syllables in a word were found not to affect performance, nor were derived nouns more difficult to process than simple nouns. It is suggested that reading aloud employs grapheme-phoneme translation based upon a letter-by-letter analysis of the stimulus: that discriminating words from non-words obeying the rules of English orthography and phonology employs a search of the lexicon based upon a holistic analysis of the stimulus; and that discriminating words from non-words violating those rules employs a direct test of the regularity of the stimulus based upon the combinatory rules of English orthography.", "PMID": 974448}
{"id": "pubmed23n0032_9892", "title": "A failure to explain the effects of false heart-rate feedback on affect by induced changes in physiological response.", "content": "An experiment was performed to validate and extend the self-persuasion hypothesis of Valins by using slide stimuli reported to create strong unpleasant feelings. The hypothesis suggests that in situations where there is no immediate explanation of induced arousal in terms of a cue of false heart-rate feedback then reported affect is dependent on a cognitive interpretation. Evidence is presented in the present study which not only supports such an interpretation but finds no support for a previous study that reported changes in affect to be a consequence of induced changes in actual physiological responses. The implication of this discrepancy from the previous reported findings are related to the possible efficiency of techniques of systematic desensitization.", "contents": "A failure to explain the effects of false heart-rate feedback on affect by induced changes in physiological response. An experiment was performed to validate and extend the self-persuasion hypothesis of Valins by using slide stimuli reported to create strong unpleasant feelings. The hypothesis suggests that in situations where there is no immediate explanation of induced arousal in terms of a cue of false heart-rate feedback then reported affect is dependent on a cognitive interpretation. Evidence is presented in the present study which not only supports such an interpretation but finds no support for a previous study that reported changes in affect to be a consequence of induced changes in actual physiological responses. The implication of this discrepancy from the previous reported findings are related to the possible efficiency of techniques of systematic desensitization.", "PMID": 974449}
{"id": "pubmed23n0032_9893", "title": "The electrodermal component of the orienting response in blind and deaf individuals.", "content": "Skin conductance response (SCR) was monitored as an index of the orienting response (OR) to a series of 20 tones presented to blind and sighted schoolchildren. Blind children showed fewer criterion SCRs than sighted children, and significantly more of the blind sample exhibited SCR habituation. The blind group also demonstrated fewer non-specific SCRs than sighted control subjects, but demonstrated a higher average skin conductance level (SCL). A second experiment found similar effects for deaf versus hearing children presented with simple visual stimulation, save that no significant difference was observed between these groups in SCL. These results can be explained by assuming that loss of modality results in more efficient development of a neuronal model of the salient features of stimuli impinging on the remaining intact modalities. An explanation in terms of the assessed significance of the stimulus situation can also be entertained. The atypical skin conductance activity of three of the blind group was given separate consideration.", "contents": "The electrodermal component of the orienting response in blind and deaf individuals. Skin conductance response (SCR) was monitored as an index of the orienting response (OR) to a series of 20 tones presented to blind and sighted schoolchildren. Blind children showed fewer criterion SCRs than sighted children, and significantly more of the blind sample exhibited SCR habituation. The blind group also demonstrated fewer non-specific SCRs than sighted control subjects, but demonstrated a higher average skin conductance level (SCL). A second experiment found similar effects for deaf versus hearing children presented with simple visual stimulation, save that no significant difference was observed between these groups in SCL. These results can be explained by assuming that loss of modality results in more efficient development of a neuronal model of the salient features of stimuli impinging on the remaining intact modalities. An explanation in terms of the assessed significance of the stimulus situation can also be entertained. The atypical skin conductance activity of three of the blind group was given separate consideration.", "PMID": 974450}
{"id": "pubmed23n0032_9894", "title": "Qualitative analysis of the performance of introverts and extraverts on Standard Progressive Matrices.", "content": "The present study was designed to bring out the differences between extraverts and introverts on Standard Progressive Matrices. A group of 100 subjects of equal number of sexes were given a qualitative analysis of their performance on SPM. The study brought out the initial superiority of extraverts over introverts. But with passage of time and increase in difficulty level the introverts did significantly better as shown both by the t-ratios and the F-ratios. Personality interacted with sex to yield differences in performance of wrong scores only. These findings were discussed in terms of arousal, cortical excitation and reactive inhibition.", "contents": "Qualitative analysis of the performance of introverts and extraverts on Standard Progressive Matrices. The present study was designed to bring out the differences between extraverts and introverts on Standard Progressive Matrices. A group of 100 subjects of equal number of sexes were given a qualitative analysis of their performance on SPM. The study brought out the initial superiority of extraverts over introverts. But with passage of time and increase in difficulty level the introverts did significantly better as shown both by the t-ratios and the F-ratios. Personality interacted with sex to yield differences in performance of wrong scores only. These findings were discussed in terms of arousal, cortical excitation and reactive inhibition.", "PMID": 974451}
{"id": "pubmed23n0032_9895", "title": "Cognitive strategies and multidimensional scaling.", "content": "The problems involved in equating multidimensional scaling solutions with cognitive strategies are considered. Empirical evidence is presented to suggest that scaling solutions are relatively insensitive to the combination rule used by subjects, and thus may be used as an indication of the basic variables, but not their method of combination, that underlie a cognitive strategy.", "contents": "Cognitive strategies and multidimensional scaling. The problems involved in equating multidimensional scaling solutions with cognitive strategies are considered. Empirical evidence is presented to suggest that scaling solutions are relatively insensitive to the combination rule used by subjects, and thus may be used as an indication of the basic variables, but not their method of combination, that underlie a cognitive strategy.", "PMID": 974452}
{"id": "pubmed23n0032_9896", "title": "Right- and left-handed counting of braille dots in subjects unaccustomed to braille.", "content": "Previous work has shown that children acquainted with Braille can usually read better with their left than with their right hand. It is also known that, at least in right-handed people, the right cerebral hemisphere is dominant for counting dots. The present paper compares, in right-handed subjects unacquainted with Braille, the comparative skill of right and left middle (M) and index (I) fingers in counting Braille dots. In one experiment with 30 subjects no significant difference was found between LI and RI fingers. Another experiment failed to reveal any R/L differences although it detected a superiority of I over M fingers at P less than 0-025. When the task was split between the two I fingers working simultaneously they performed significantly (P less than 0-001) better than when working alone. This latter finding is thought to have a bearing on the failure to detect R/L differences; other possible explanations of the findings are also discussed.", "contents": "Right- and left-handed counting of braille dots in subjects unaccustomed to braille. Previous work has shown that children acquainted with Braille can usually read better with their left than with their right hand. It is also known that, at least in right-handed people, the right cerebral hemisphere is dominant for counting dots. The present paper compares, in right-handed subjects unacquainted with Braille, the comparative skill of right and left middle (M) and index (I) fingers in counting Braille dots. In one experiment with 30 subjects no significant difference was found between LI and RI fingers. Another experiment failed to reveal any R/L differences although it detected a superiority of I over M fingers at P less than 0-025. When the task was split between the two I fingers working simultaneously they performed significantly (P less than 0-001) better than when working alone. This latter finding is thought to have a bearing on the failure to detect R/L differences; other possible explanations of the findings are also discussed.", "PMID": 974453}
{"id": "pubmed23n0032_9897", "title": "Selective renal occlusion phlebography with a balloon catheter.", "content": "Technique of selective renal occlusion phlebography is described. For the temporary occlusion of the renal vein a Swan-Ganz or Dotter-Lucas balloon catheter are used. The balloon catheter is directed into the renal vein using a Desilets-Hoffmann or EDSLAB introducer. Occlusion of the renal vein is achieved by insufflation of 2-3 ml. air into the balloon of the catheter. Selective renal occlusion phlebography leads to optimal retrograde opacification of the renal venous system.", "contents": "Selective renal occlusion phlebography with a balloon catheter. Technique of selective renal occlusion phlebography is described. For the temporary occlusion of the renal vein a Swan-Ganz or Dotter-Lucas balloon catheter are used. The balloon catheter is directed into the renal vein using a Desilets-Hoffmann or EDSLAB introducer. Occlusion of the renal vein is achieved by insufflation of 2-3 ml. air into the balloon of the catheter. Selective renal occlusion phlebography leads to optimal retrograde opacification of the renal venous system.", "PMID": 974455}
{"id": "pubmed23n0032_9898", "title": "Radiological manifestations of oesophageal involvement in acanthosis nigricans.", "content": "Radiologic features of two cases of acanthosis nigricans with oesophageal involvement are reported. The first case demonstrated diffuse, granular shadows throughout the oesophagus which were difficult to differentiate from oesophageal moniliasis. Another case showed many discrete and tiny elevations resembling pseudopolyposis of the colon. Emphasis is placed on radiological differential diagnosis from moniliasis and leukoplakia of the oesophagus.", "contents": "Radiological manifestations of oesophageal involvement in acanthosis nigricans. Radiologic features of two cases of acanthosis nigricans with oesophageal involvement are reported. The first case demonstrated diffuse, granular shadows throughout the oesophagus which were difficult to differentiate from oesophageal moniliasis. Another case showed many discrete and tiny elevations resembling pseudopolyposis of the colon. Emphasis is placed on radiological differential diagnosis from moniliasis and leukoplakia of the oesophagus.", "PMID": 974456}
{"id": "pubmed23n0032_9899", "title": "Pineal gland calcification (PGC) in Ugandans. A radiological study of 200 isolated pineal glands.", "content": "Two hundred formalin-fixed pineal glands from consecutive unselected post-mortems on Ugandan Africans have been X-rayed. The degree of pineal gland calcification has been divided into four stages and it is shown that 43 per cent of all pineal glands after the age of ten years are likely to be detected in an ordinary skull X ray. This high percentage of calcification contrasts with the previously reported low figure from races other than whites. The pineal glands from females were more often calcified and heavier than those from males; however, the stalks of pineal glands from males were calcified more frequently than those of females. The average weight per decade was almost constant. In Ugandan Africans the pineal glands were significantly lighter than in Caucasians, and a possible reason for this is briefly discussed.", "contents": "Pineal gland calcification (PGC) in Ugandans. A radiological study of 200 isolated pineal glands. Two hundred formalin-fixed pineal glands from consecutive unselected post-mortems on Ugandan Africans have been X-rayed. The degree of pineal gland calcification has been divided into four stages and it is shown that 43 per cent of all pineal glands after the age of ten years are likely to be detected in an ordinary skull X ray. This high percentage of calcification contrasts with the previously reported low figure from races other than whites. The pineal glands from females were more often calcified and heavier than those from males; however, the stalks of pineal glands from males were calcified more frequently than those of females. The average weight per decade was almost constant. In Ugandan Africans the pineal glands were significantly lighter than in Caucasians, and a possible reason for this is briefly discussed.", "PMID": 974457}
{"id": "pubmed23n0032_9900", "title": "\"Vascular\" cerebral infarctions demonstrated by serial gamma-camera scinti photography.", "content": "The apparently paradoxical appearance of increased vascularity appearing in the radionuclide angiographic studies of a patient with cerebral infarction has recently been described and attributed to the \"luxury perfusion syndrome\". It is suggested that this phenomenon occurs more frequently than previously thought and in fact has been observed in nine patients presenting for a cerebral scan during a ten-month period. These cases are reviewed and an alternative explanation for the occurrence of increased vascularity on the dynamic study is submitted.", "contents": "\"Vascular\" cerebral infarctions demonstrated by serial gamma-camera scinti photography. The apparently paradoxical appearance of increased vascularity appearing in the radionuclide angiographic studies of a patient with cerebral infarction has recently been described and attributed to the \"luxury perfusion syndrome\". It is suggested that this phenomenon occurs more frequently than previously thought and in fact has been observed in nine patients presenting for a cerebral scan during a ten-month period. These cases are reviewed and an alternative explanation for the occurrence of increased vascularity on the dynamic study is submitted.", "PMID": 974458}
{"id": "pubmed23n0032_9901", "title": "Computerized axial tomography: estimation of spatial and density resolution capability.", "content": "The spatial and density resolution capability of the EMI-Scanner device for computerized axial tomography has been determined in vitro. For density differences greater than +/-1 per cent the spatial resolution is 6 X 6 mm. For density differences of 3 per cent and greater the resolution is 3 X 3 mm. Density resolution is at least +/-1 per cent for objects greater than 1 cm. Preliminary data on in vitro measurement of X-ray linear attenuation coefficients in tissue biopsies and standard solutions are given, together with the early results of enhancement of tissue density differences in vivo. Implications of this new technique for an in vivo neuropathology are suggested.", "contents": "Computerized axial tomography: estimation of spatial and density resolution capability. The spatial and density resolution capability of the EMI-Scanner device for computerized axial tomography has been determined in vitro. For density differences greater than +/-1 per cent the spatial resolution is 6 X 6 mm. For density differences of 3 per cent and greater the resolution is 3 X 3 mm. Density resolution is at least +/-1 per cent for objects greater than 1 cm. Preliminary data on in vitro measurement of X-ray linear attenuation coefficients in tissue biopsies and standard solutions are given, together with the early results of enhancement of tissue density differences in vivo. Implications of this new technique for an in vivo neuropathology are suggested.", "PMID": 974459}
{"id": "pubmed23n0032_9902", "title": "A comparison of clinical results in brain scanning using germanium semiconductor detectors and sodium iodide detectors.", "content": "A comparison is presented of the image quality obtained with a 70 mm Ge(Li) detector scanner and with routine techniques based on NaI (Tl) detectors. One hundred and sixty-five pairs of brain scans have been examined for which the patients have been scanned with both Ge(Li) and NaI (Tl) detectors. It is concluded that no major difficulties exist in introducing Ge(Li) scanners and that while a comparison of scans from different instruments is difficult there is evidence that improved diagnostic information may be obtained from scanners with Ge(Li) detectors.", "contents": "A comparison of clinical results in brain scanning using germanium semiconductor detectors and sodium iodide detectors. A comparison is presented of the image quality obtained with a 70 mm Ge(Li) detector scanner and with routine techniques based on NaI (Tl) detectors. One hundred and sixty-five pairs of brain scans have been examined for which the patients have been scanned with both Ge(Li) and NaI (Tl) detectors. It is concluded that no major difficulties exist in introducing Ge(Li) scanners and that while a comparison of scans from different instruments is difficult there is evidence that improved diagnostic information may be obtained from scanners with Ge(Li) detectors.", "PMID": 974460}
{"id": "pubmed23n0032_9903", "title": "Scanning electron microscopy of mouse intestinal mucosa after cobalt 60 and D-T neutron irradiation.", "content": "The stem-cell population of the intestinal crypt is an important model system in experimental radiobiology. Standardized techniques have been developed to allow quantitation of the response of crypt cells to radiation injury following doses of 0-2 krad of D-T neutrons or 60Co gamma rays. These techniques rely on the identification of regenerating crypt cells three-and-a-half days after irradiation. The results are expressed as the number of regenerating crypts per circumference of small intestine, as determined by conventional histological examination; the more profound the injury, the smaller the crypt count. The practical relevance of crypt-counting techniques to clinical radiotherapy is limited by their relative insensitivity; the dose levels commonly used in fractionated radiotherapy produce no detectable response. Scanning electron microscopy of the mucosal surface provides a more sensitive measure of radiation injury. The earliest detectable changes occur at the level of 300 rad of gamma radiation, well below the threshold of the crypt-counting technique. At around 1,000 rad, where the first drop in crypt counts occurs, there are well-marked morphological changes which become more severe with increasing dose levels. Some differences have been observed between the morphological effects of gamma and neutron irradiation at points of radiobiological equivalence in terms of crypt counts (using an RBE value of about 2). The changes observed may reflect more than the disruption of epithelial cell kinetics. Mucosal morphology is the total expression of many different biological parameters of which the regenerative ability of the crypt cells is only one. The surface microanatomy of the gut may be the most sensitive indicator of radiation injury which is conveniently available for study.", "contents": "Scanning electron microscopy of mouse intestinal mucosa after cobalt 60 and D-T neutron irradiation. The stem-cell population of the intestinal crypt is an important model system in experimental radiobiology. Standardized techniques have been developed to allow quantitation of the response of crypt cells to radiation injury following doses of 0-2 krad of D-T neutrons or 60Co gamma rays. These techniques rely on the identification of regenerating crypt cells three-and-a-half days after irradiation. The results are expressed as the number of regenerating crypts per circumference of small intestine, as determined by conventional histological examination; the more profound the injury, the smaller the crypt count. The practical relevance of crypt-counting techniques to clinical radiotherapy is limited by their relative insensitivity; the dose levels commonly used in fractionated radiotherapy produce no detectable response. Scanning electron microscopy of the mucosal surface provides a more sensitive measure of radiation injury. The earliest detectable changes occur at the level of 300 rad of gamma radiation, well below the threshold of the crypt-counting technique. At around 1,000 rad, where the first drop in crypt counts occurs, there are well-marked morphological changes which become more severe with increasing dose levels. Some differences have been observed between the morphological effects of gamma and neutron irradiation at points of radiobiological equivalence in terms of crypt counts (using an RBE value of about 2). The changes observed may reflect more than the disruption of epithelial cell kinetics. Mucosal morphology is the total expression of many different biological parameters of which the regenerative ability of the crypt cells is only one. The surface microanatomy of the gut may be the most sensitive indicator of radiation injury which is conveniently available for study.", "PMID": 974461}
{"id": "pubmed23n0032_9904", "title": "Measurements of neutron spectra at depths in tissue equivalent material for fast neutron beams generated by deuterons on beryllium.", "content": "The threshold foil technique has been used to investigate the behaviour in tissue of the spectra of neutron beams produced by the bombardment of thick Be targets with 13-0 and 20-5 MeV deuterons. The results indicate a gradual \"softening\" with depth in tissue of the spectrum generated by 20-5 MeV deutrons which contrasts with a gradual hardening as reported by other authors. This is discussed in terms of the significance of field size and the methods used for data collection and analysis.", "contents": "Measurements of neutron spectra at depths in tissue equivalent material for fast neutron beams generated by deuterons on beryllium. The threshold foil technique has been used to investigate the behaviour in tissue of the spectra of neutron beams produced by the bombardment of thick Be targets with 13-0 and 20-5 MeV deuterons. The results indicate a gradual \"softening\" with depth in tissue of the spectrum generated by 20-5 MeV deutrons which contrasts with a gradual hardening as reported by other authors. This is discussed in terms of the significance of field size and the methods used for data collection and analysis.", "PMID": 974462}
{"id": "pubmed23n0032_9905", "title": "Grey-scale ultrasonic imaging of the kidney.", "content": "The ultrasonic appearances of the kidney in images which contain grey shades corresponding to the detected echo-pulse heights have been examined. These \"greyscale\" images were obtained using a standard Nuclear Enterprises Diasonograph 4102B and a Varian 620/L digital computer interfaced to this Diasonograph. The computer was used to analyse and display the ultrasonograms with 16 grey shades. Both kidneys of 36 subjects were examined and compared with IVU findings. This group included patients with normal kidneys, polycystic kidneys, hydronephrosis, glomerulonephritis and neoplastic tumours. It was found possible to make sharp, grey-shaded ultrasonograms which were very useful to the diagnostician. In particular, the grey shade of the renal parenchyma was used as a standard to compare other grey-shaded structures. Also the grey shading was useful in defining the renal pelvis in transverse scans and the renal poles in axial scan.", "contents": "Grey-scale ultrasonic imaging of the kidney. The ultrasonic appearances of the kidney in images which contain grey shades corresponding to the detected echo-pulse heights have been examined. These \"greyscale\" images were obtained using a standard Nuclear Enterprises Diasonograph 4102B and a Varian 620/L digital computer interfaced to this Diasonograph. The computer was used to analyse and display the ultrasonograms with 16 grey shades. Both kidneys of 36 subjects were examined and compared with IVU findings. This group included patients with normal kidneys, polycystic kidneys, hydronephrosis, glomerulonephritis and neoplastic tumours. It was found possible to make sharp, grey-shaded ultrasonograms which were very useful to the diagnostician. In particular, the grey shade of the renal parenchyma was used as a standard to compare other grey-shaded structures. Also the grey shading was useful in defining the renal pelvis in transverse scans and the renal poles in axial scan.", "PMID": 974463}
{"id": "pubmed23n0032_9906", "title": "Estimation of glomerular size and number from radiographs of the kidney.", "content": "In 32 adult human kidneys obtained at necropsy, renal demensions, measured from radiographs, were correlated with the number and cross-sectional area of glomeruli, determined by point-counting and computerized image analysis. Cortical area, measured from post-mortem angiograms, was poorly correlated with glomerular area and was not significantly correlated with glomerular number per kidney. The area of the whole kidney was poorly correlated with the number of glomeruli per kidney and was not significantly correlated with glomerular area. However renal dimensions, particularly total renal area, were highly significantly correlated with the product of glomerular area and number. This may allow glomerular numbers to be estimated in life but will not assess loss of glon the ageing kidney, the number of glomeruli per unit volume increased.", "contents": "Estimation of glomerular size and number from radiographs of the kidney. In 32 adult human kidneys obtained at necropsy, renal demensions, measured from radiographs, were correlated with the number and cross-sectional area of glomeruli, determined by point-counting and computerized image analysis. Cortical area, measured from post-mortem angiograms, was poorly correlated with glomerular area and was not significantly correlated with glomerular number per kidney. The area of the whole kidney was poorly correlated with the number of glomeruli per kidney and was not significantly correlated with glomerular area. However renal dimensions, particularly total renal area, were highly significantly correlated with the product of glomerular area and number. This may allow glomerular numbers to be estimated in life but will not assess loss of glon the ageing kidney, the number of glomeruli per unit volume increased.", "PMID": 974469}
{"id": "pubmed23n0032_9907", "title": "The pituitary fossa in Cushing's syndrome. A retrospective analysis of 93 patients.", "content": "A  retrospective analysis of the pituitary fossa in 93 patients with Cushing's syndrome is presented. Radiological evidence of a pituitary tumour was found in 20 of 86 patients with pituitary-dependent disease (23%). In 11 of the 20 patients, the diagnosis depended on analysis of lateral tomograms. None of the seven patients with disease which was not pituitary-dependent had evidence of an abnormal sella. One of 20 controls without known endocrine disease had an abnormal sella.", "contents": "The pituitary fossa in Cushing's syndrome. A retrospective analysis of 93 patients. A  retrospective analysis of the pituitary fossa in 93 patients with Cushing's syndrome is presented. Radiological evidence of a pituitary tumour was found in 20 of 86 patients with pituitary-dependent disease (23%). In 11 of the 20 patients, the diagnosis depended on analysis of lateral tomograms. None of the seven patients with disease which was not pituitary-dependent had evidence of an abnormal sella. One of 20 controls without known endocrine disease had an abnormal sella.", "PMID": 974471}
{"id": "pubmed23n0032_9908", "title": "A quantitative study of the EMI values obtained for normal brain cerebral infarction and certain tumours.", "content": "A quantitative study has been made of the EMI numbers of normal brain, cerebral infarction and certain tumours. The scans were recorded on magnetic tape and analysed using a minicomuter linked to a graphic display unit. This system not only yielded 16 grey scales compared with the ten currently available, but was programmed to allow selected regions of the scans to be outlined. From these regions the computer calculated the area, the mean EMI number and its standard deviation. It was found that in 15 normal brain scans, the EMI values obtained for normal frontal and temporal lobes were similar, but that the values for the basal ganglia and occipital lobes were significantly different from the first two regions and from each other. Ten cases of cerebral infarction and 30 cases of cerebral tumour were analysed, and it was shown that analysing representative areas was more informative than surveying the whole lesion. Whilst only half of the scans of brain tumours had a significantly altered EMI number compared with that of normal brain, enhancement of tumour density with sodium iothalamate revealed a consistent and significant elevation of the EMI number for all tumours. In particular, the value for enhanced meningiomas was almost double and malignant tumours more than a third larger than normal brain. It was not possible to differentiate quantitatively between astrocytomas and metastases.", "contents": "A quantitative study of the EMI values obtained for normal brain cerebral infarction and certain tumours. A quantitative study has been made of the EMI numbers of normal brain, cerebral infarction and certain tumours. The scans were recorded on magnetic tape and analysed using a minicomuter linked to a graphic display unit. This system not only yielded 16 grey scales compared with the ten currently available, but was programmed to allow selected regions of the scans to be outlined. From these regions the computer calculated the area, the mean EMI number and its standard deviation. It was found that in 15 normal brain scans, the EMI values obtained for normal frontal and temporal lobes were similar, but that the values for the basal ganglia and occipital lobes were significantly different from the first two regions and from each other. Ten cases of cerebral infarction and 30 cases of cerebral tumour were analysed, and it was shown that analysing representative areas was more informative than surveying the whole lesion. Whilst only half of the scans of brain tumours had a significantly altered EMI number compared with that of normal brain, enhancement of tumour density with sodium iothalamate revealed a consistent and significant elevation of the EMI number for all tumours. In particular, the value for enhanced meningiomas was almost double and malignant tumours more than a third larger than normal brain. It was not possible to differentiate quantitatively between astrocytomas and metastases.", "PMID": 974472}
{"id": "pubmed23n0032_9909", "title": "Combination of isotope venography and lung scanning.", "content": "Seventy patients were investigated by isotope venography of the lower leg veigns and pelvis using 99Tcm human serum albumin, mainly as microspheres, in the course of routine lung scanning. In 54 of these patients, 52 pelvic, and 98 conventional ascending X-ray contrast venograms were performed for comparison, ninety three per cent showed good correlation, and 4% poor correlation. There were 2% false positive and 1% false negative isotope examinations. Isotope venography proved to be a painless, safe and reliable method for the detection of deep vein thrombosis. Its correlation with contrast venography is high, especially for the upper thigh and pelvis. It can be performed in 30 minutes, including standard lung scan, and can be repeated in patients to assess response to treatment, saving the patient the discomfort and radiation of comparable radiological examinations.", "contents": "Combination of isotope venography and lung scanning. Seventy patients were investigated by isotope venography of the lower leg veigns and pelvis using 99Tcm human serum albumin, mainly as microspheres, in the course of routine lung scanning. In 54 of these patients, 52 pelvic, and 98 conventional ascending X-ray contrast venograms were performed for comparison, ninety three per cent showed good correlation, and 4% poor correlation. There were 2% false positive and 1% false negative isotope examinations. Isotope venography proved to be a painless, safe and reliable method for the detection of deep vein thrombosis. Its correlation with contrast venography is high, especially for the upper thigh and pelvis. It can be performed in 30 minutes, including standard lung scan, and can be repeated in patients to assess response to treatment, saving the patient the discomfort and radiation of comparable radiological examinations.", "PMID": 974473}
{"id": "pubmed23n0032_9910", "title": "Ultrasound in the management of acute pancreatitis.", "content": "Thirty-eight patients have been examined by ultrasound when symptoms or sign suggested the development of a pseudocyst following an attack of proven acute pancreatitis. Pseudocyst was diagnosed in 23 of the 38 cases. Five patients had multilocular cysts, four of which were shown to be communicating. Laparotomy was carried out on 14 of the 23 patients and surgical drainage was performed in 12 cases. The remaining nine cases were monitored and showed steady regression. Small cysts arising in the head of pancreas may give rise to recurrent or persistent pancreatitis and may be demonstrated pre-operatively by ultrasound but not readily by other means. A further 12 patients showed an area of irregular absorption of ultrasound interpreted as an inflammatory mass. Monitoring of these cases showed progressive resolution without cyst formation. Three of these cases subsequently required laparotomy-one developed an abscess and one necrosis of the body and tail of pancreas while a third developed severe pancreatic fibrosis of the area identified by ultrasound. The ability to distinguish between pseudocysts and inflammatory masses and to demonstrate communication between multiple cysts is of considerable value in pre-operative diagnosis.", "contents": "Ultrasound in the management of acute pancreatitis. Thirty-eight patients have been examined by ultrasound when symptoms or sign suggested the development of a pseudocyst following an attack of proven acute pancreatitis. Pseudocyst was diagnosed in 23 of the 38 cases. Five patients had multilocular cysts, four of which were shown to be communicating. Laparotomy was carried out on 14 of the 23 patients and surgical drainage was performed in 12 cases. The remaining nine cases were monitored and showed steady regression. Small cysts arising in the head of pancreas may give rise to recurrent or persistent pancreatitis and may be demonstrated pre-operatively by ultrasound but not readily by other means. A further 12 patients showed an area of irregular absorption of ultrasound interpreted as an inflammatory mass. Monitoring of these cases showed progressive resolution without cyst formation. Three of these cases subsequently required laparotomy-one developed an abscess and one necrosis of the body and tail of pancreas while a third developed severe pancreatic fibrosis of the area identified by ultrasound. The ability to distinguish between pseudocysts and inflammatory masses and to demonstrate communication between multiple cysts is of considerable value in pre-operative diagnosis.", "PMID": 974474}
{"id": "pubmed23n0032_9911", "title": "A computer-based radiotherapy clinical record system using mark-sense forms.", "content": "A computer-based system for radiotherapy clinical records is described. Its main features are the use of a single mark-sense form for input of data at all stages of the patient's progress, and the provision of a plain language print-out of the patient's record after each updating. The programs are written in Fortran and are implemented on a Rank Xerox Sigma 6 computer.", "contents": "A computer-based radiotherapy clinical record system using mark-sense forms. A computer-based system for radiotherapy clinical records is described. Its main features are the use of a single mark-sense form for input of data at all stages of the patient's progress, and the provision of a plain language print-out of the patient's record after each updating. The programs are written in Fortran and are implemented on a Rank Xerox Sigma 6 computer.", "PMID": 974475}
{"id": "pubmed23n0032_9912", "title": "An on-line computer graphics terminal for radiotherapy treatment planning.", "content": "A programmed graphics terminal has been connected on-line to a large time-shared computer for calculating dose distributions in radiotherapy treatment planning and provides a viable alternative to dedicated systems and batch working. The terminal equipment is based on a mini-computer and includes a function-key devise for outline input, a large-screen refresh oscilloscope for viewing results and an X-Y plotter for hard-copy. Radiotherapy dose computation programs in standard Fortran are stored and run on a large remote computer with graphical interaction at the terminal. External beam programs can calculate dose distributions for most commonly used treatment situations and can compute in off-axis planes. Data input is fully interactive and easy to understnad. Dose distributions are displayed as isodose contours. Advantages of the system include accuracy, speed, ease of use and maintenance, and transferability of the programs between different host computers.", "contents": "An on-line computer graphics terminal for radiotherapy treatment planning. A programmed graphics terminal has been connected on-line to a large time-shared computer for calculating dose distributions in radiotherapy treatment planning and provides a viable alternative to dedicated systems and batch working. The terminal equipment is based on a mini-computer and includes a function-key devise for outline input, a large-screen refresh oscilloscope for viewing results and an X-Y plotter for hard-copy. Radiotherapy dose computation programs in standard Fortran are stored and run on a large remote computer with graphical interaction at the terminal. External beam programs can calculate dose distributions for most commonly used treatment situations and can compute in off-axis planes. Data input is fully interactive and easy to understnad. Dose distributions are displayed as isodose contours. Advantages of the system include accuracy, speed, ease of use and maintenance, and transferability of the programs between different host computers.", "PMID": 974476}
{"id": "pubmed23n0032_9913", "title": "Persisting cyclic ovarian activity in cervical cancer after surgical transposition of the ovaries and pelvic irradiation.", "content": "The effect of surgical transposition of the ovaries on gonadal function was investigated in ten young women with carcinoma of the uterine cervix, stages I and II. Gonadotrophin-, oestradiol- and progesterone-levels were determined before and during pelvic irradiation. A control group consisted of seven patients with cervical cancer who did not undergo ovaria transposition. In the control group gonadotrophins began to rise after radiation doses of 560-2400 rad (FSH) and 1130-2600 rad (LH) respectively. The excessive secretion was not seen in patients who had ovarian lifting. Ovulatory cycles occurred during or after pelvic irradiation in seven women of the study group, either spontaneously or induced by clomiphene treatment. This indicates that transposition preceding radiotherapy is an effective means of preserving ovarian secretion in young women in whom malignancies of the pelvic region demand irradiation.", "contents": "Persisting cyclic ovarian activity in cervical cancer after surgical transposition of the ovaries and pelvic irradiation. The effect of surgical transposition of the ovaries on gonadal function was investigated in ten young women with carcinoma of the uterine cervix, stages I and II. Gonadotrophin-, oestradiol- and progesterone-levels were determined before and during pelvic irradiation. A control group consisted of seven patients with cervical cancer who did not undergo ovaria transposition. In the control group gonadotrophins began to rise after radiation doses of 560-2400 rad (FSH) and 1130-2600 rad (LH) respectively. The excessive secretion was not seen in patients who had ovarian lifting. Ovulatory cycles occurred during or after pelvic irradiation in seven women of the study group, either spontaneously or induced by clomiphene treatment. This indicates that transposition preceding radiotherapy is an effective means of preserving ovarian secretion in young women in whom malignancies of the pelvic region demand irradiation.", "PMID": 974477}
{"id": "pubmed23n0032_9914", "title": "Long-term low-dose co-trimoxazole in prophylaxis of childhood urinary tract infection: clinical aspects.", "content": "Long-term low-dosage prophylaxis may be used in children with recurrent urinary tract infection to prevent reinfection of the urinary tract while the underlying cause of infection persists. Co-trimoxazole in a dose of 2 mg trimethoprin combined with 10 mg sulphamethoxazole per kg body weight daily has proved very effective: only six of 130 children receiving this treatment during a total period of 2637 months developed a reinfection. Co-trimoxazole was acceptable, compliance was good, and there were no important adverse effects. Supportive measures during prophylaxis are important. Sixty-five children were follow up after completion of their co-trimoxazole prophylaxis. Twenty-seven developed reinfections with fresh organisms, over two-thirds occurring within three months of discontinuing prophylaxis. Each one of these reinfections was sensitive to trimethoprin. The rectal flora were similarly sensitive.", "contents": "Long-term low-dose co-trimoxazole in prophylaxis of childhood urinary tract infection: clinical aspects. Long-term low-dosage prophylaxis may be used in children with recurrent urinary tract infection to prevent reinfection of the urinary tract while the underlying cause of infection persists. Co-trimoxazole in a dose of 2 mg trimethoprin combined with 10 mg sulphamethoxazole per kg body weight daily has proved very effective: only six of 130 children receiving this treatment during a total period of 2637 months developed a reinfection. Co-trimoxazole was acceptable, compliance was good, and there were no important adverse effects. Supportive measures during prophylaxis are important. Sixty-five children were follow up after completion of their co-trimoxazole prophylaxis. Twenty-seven developed reinfections with fresh organisms, over two-thirds occurring within three months of discontinuing prophylaxis. Each one of these reinfections was sensitive to trimethoprin. The rectal flora were similarly sensitive.", "PMID": 974492}
{"id": "pubmed23n0032_9915", "title": "Long-term low-dose co-trimoxazole in prophylaxis of childhood urinary tract infection: bacteriological aspects.", "content": "The bacteriological consequences of giving long-term low-dose co-trimoxazole to children to prevent reinfection of the urinary tract were studied. Only six \"break-through\" infections occurred during 2637 child-months of prophylaxis. The children complied well with treatment. During prophylaxis the number of rectal coliform bacilli recovered was greatly and rapidly reduced, but at least 70% of the surviving coliform organisms remained sensitive to the two components of co-trimoxazole. Changes in sensitivity pattern were evident within a month of starting treatment and the proportion of rectal organisms resistant to sulphonamide or trimethoprim did not increase with time. After stopping co-trimoxazole prophylaxis the number of rectal organisms recoverable returned rapidly to normal, as did their sensitivities to trimethoprim and sulphonamide. Further episodes of urinary tract infection developing after prophylaxis was stopped were caused by organisms sensitive to a wide range of antimicrobial agents, including trimethoprim.", "contents": "Long-term low-dose co-trimoxazole in prophylaxis of childhood urinary tract infection: bacteriological aspects. The bacteriological consequences of giving long-term low-dose co-trimoxazole to children to prevent reinfection of the urinary tract were studied. Only six \"break-through\" infections occurred during 2637 child-months of prophylaxis. The children complied well with treatment. During prophylaxis the number of rectal coliform bacilli recovered was greatly and rapidly reduced, but at least 70% of the surviving coliform organisms remained sensitive to the two components of co-trimoxazole. Changes in sensitivity pattern were evident within a month of starting treatment and the proportion of rectal organisms resistant to sulphonamide or trimethoprim did not increase with time. After stopping co-trimoxazole prophylaxis the number of rectal organisms recoverable returned rapidly to normal, as did their sensitivities to trimethoprim and sulphonamide. Further episodes of urinary tract infection developing after prophylaxis was stopped were caused by organisms sensitive to a wide range of antimicrobial agents, including trimethoprim.", "PMID": 974493}
{"id": "pubmed23n0032_9916", "title": "Thyrotoxic vomiting.", "content": "In seven patients vomiting played an important part in the presentation of thyrotoxicosis. Vomiting does not always indicate severe thyroid disease, and the diagnosis in patients presenting with this symptom may be long delayed.", "contents": "Thyrotoxic vomiting. In seven patients vomiting played an important part in the presentation of thyrotoxicosis. Vomiting does not always indicate severe thyroid disease, and the diagnosis in patients presenting with this symptom may be long delayed.", "PMID": 974494}
{"id": "pubmed23n0032_9917", "title": "Mode of presentation of juvenile diabetes.", "content": "In a study of 66 children with juvenile diabetes symptoms had often present for over a month before diagnosis. Though polyuria, polydipsia, and weight loss were the commonest features, other important symptoms included tiredness lethargy, and malaise. A gradual onset of diabetes was commoner than is generally realised.", "contents": "Mode of presentation of juvenile diabetes. In a study of 66 children with juvenile diabetes symptoms had often present for over a month before diagnosis. Though polyuria, polydipsia, and weight loss were the commonest features, other important symptoms included tiredness lethargy, and malaise. A gradual onset of diabetes was commoner than is generally realised.", "PMID": 974495}
{"id": "pubmed23n0032_9918", "title": "How trustworthy are bowel histories? Comparison of recalled and recorded information.", "content": "One hundred and fifty hospital outpatients were questioned about their bowel habits and then asked to record these in diary booklets for two weeks. Overall, recalled and recorded figures for frequency of defecation agreed fairly closely, but in 16% of patients there was a discrepancy of three or more bowel actions per week. This was usually an exaggeration of the difference from the norm of one a day. Patients were bad at predicting episodes of changed bowel frequency. These findings cast doubt on the value of population surveys of bowel habit based solely on questionnaires. They also suggest that the irritable bowel syndrome might be correctly diagnosed more often if patients were routinely asked to record their bowel actions.", "contents": "How trustworthy are bowel histories? Comparison of recalled and recorded information. One hundred and fifty hospital outpatients were questioned about their bowel habits and then asked to record these in diary booklets for two weeks. Overall, recalled and recorded figures for frequency of defecation agreed fairly closely, but in 16% of patients there was a discrepancy of three or more bowel actions per week. This was usually an exaggeration of the difference from the norm of one a day. Patients were bad at predicting episodes of changed bowel frequency. These findings cast doubt on the value of population surveys of bowel habit based solely on questionnaires. They also suggest that the irritable bowel syndrome might be correctly diagnosed more often if patients were routinely asked to record their bowel actions.", "PMID": 974496}
{"id": "pubmed23n0032_9919", "title": "Comparative study of ultrasound, 131I-19-iodocholesterol scintigraphy, and aortography in localising adrenal lesions.", "content": "Twenty-seven consecutive patients with endocrinological disease necessitating adrenal surgery underwent blind preoperative investigation with ultrasound, renal aortography, and adrenocortical scintigraphy for an adrenal lesion. Nine patients had pituitary-dependent Cushing's syndrome, five had pituitary-independent Cushing's syndrome, four had an adrenocortical androgenic excess, and nine had a preoperative diagnosis of phaeochromocytoma. The predictive value of preoperative ultrasound was 100% for a positive finding and 79% for a negative result. Preoperative aortography had a predictive value of 83% for a positive finding and 64% for a negative result; and the predictive value of adrenocortical scintigraphy was 100% for a positive finding and 85% for a negative finding. In localising biochemically suspected adrenal lesions ultrasound should be the first choice, since it is rapid, noninvasive, cheap, and reasonably accurate. Adrenocortical scintigraphy has a similar diagnostic value, especially in Cushing's syndrome, but it is time consuming. Nevertheless, it may be preferable for diagnosing small glucocorticoid-secreting adenomas. Aortography should be reserved for cases with inconclusive diagnoses and suspected extra-adrenal phaeochromocytomas.", "contents": "Comparative study of ultrasound, 131I-19-iodocholesterol scintigraphy, and aortography in localising adrenal lesions. Twenty-seven consecutive patients with endocrinological disease necessitating adrenal surgery underwent blind preoperative investigation with ultrasound, renal aortography, and adrenocortical scintigraphy for an adrenal lesion. Nine patients had pituitary-dependent Cushing's syndrome, five had pituitary-independent Cushing's syndrome, four had an adrenocortical androgenic excess, and nine had a preoperative diagnosis of phaeochromocytoma. The predictive value of preoperative ultrasound was 100% for a positive finding and 79% for a negative result. Preoperative aortography had a predictive value of 83% for a positive finding and 64% for a negative result; and the predictive value of adrenocortical scintigraphy was 100% for a positive finding and 85% for a negative finding. In localising biochemically suspected adrenal lesions ultrasound should be the first choice, since it is rapid, noninvasive, cheap, and reasonably accurate. Adrenocortical scintigraphy has a similar diagnostic value, especially in Cushing's syndrome, but it is time consuming. Nevertheless, it may be preferable for diagnosing small glucocorticoid-secreting adenomas. Aortography should be reserved for cases with inconclusive diagnoses and suspected extra-adrenal phaeochromocytomas.", "PMID": 974527}
{"id": "pubmed23n0032_9920", "title": "Overnight urinary 11-hydroxycorticosteroid estimations in diagnosis of Cushing's syndrome.", "content": "Overnight specimens of urine were collected from control, obese, and hirsute women and from 11 women with Cushing's syndrome. Urinary 11-hydroxycorticosteroid levels in the group with Cushing's syndrome were significantly higher than in the other three groups. This simple test has proved invaluable when screening for Cushing's syndrome in a busy outpatient clinic.", "contents": "Overnight urinary 11-hydroxycorticosteroid estimations in diagnosis of Cushing's syndrome. Overnight specimens of urine were collected from control, obese, and hirsute women and from 11 women with Cushing's syndrome. Urinary 11-hydroxycorticosteroid levels in the group with Cushing's syndrome were significantly higher than in the other three groups. This simple test has proved invaluable when screening for Cushing's syndrome in a busy outpatient clinic.", "PMID": 974528}
{"id": "pubmed23n0032_9921", "title": "Enhanced HBsAb production in pathogenesis of fulminant viral hepatitis type B.", "content": "The possible importance of humoral immunity in the pathogenesis of fulminant hepatitis was investigated by comparing 17 patients with fulminant hepatitis type B with 20 patients with severe but non-fulminant disease. Hepatitis B surface antigen (HBsAg) was cleared from the serum significantly faster (P less than 0-001) in those with fulminant hepatitis, and in 41% anti-HBsAg (HBsAb) was detectable by radioimmunoassay (RIA) at presentation. In all 11 sera from patients with fulminant hepatitis that were examined by electron microscopy aggregates of HBsAg and HBsAb were seen. In contrast, HBsAb was never detected by RIA in those with non-fulminant hepatitis, and in only one serum specimen (5%) were aggregates seen on electron microscopy. A significant sex difference between fulminant and non-fulminant hepatitis was observed, 65% of patients with fulminant hepatitis but only 15% of patients with non-fulminant hepatitis being women (P less than 0-01). An enhanced production of HBsAb in fulminant hepatitis, by leading to free HBsAb in portal blood, may cause an Arthus reaction in the sinusoids of the liver with ensuing ischaemic necrosis of hepatocytes.", "contents": "Enhanced HBsAb production in pathogenesis of fulminant viral hepatitis type B. The possible importance of humoral immunity in the pathogenesis of fulminant hepatitis was investigated by comparing 17 patients with fulminant hepatitis type B with 20 patients with severe but non-fulminant disease. Hepatitis B surface antigen (HBsAg) was cleared from the serum significantly faster (P less than 0-001) in those with fulminant hepatitis, and in 41% anti-HBsAg (HBsAb) was detectable by radioimmunoassay (RIA) at presentation. In all 11 sera from patients with fulminant hepatitis that were examined by electron microscopy aggregates of HBsAg and HBsAb were seen. In contrast, HBsAb was never detected by RIA in those with non-fulminant hepatitis, and in only one serum specimen (5%) were aggregates seen on electron microscopy. A significant sex difference between fulminant and non-fulminant hepatitis was observed, 65% of patients with fulminant hepatitis but only 15% of patients with non-fulminant hepatitis being women (P less than 0-01). An enhanced production of HBsAb in fulminant hepatitis, by leading to free HBsAb in portal blood, may cause an Arthus reaction in the sinusoids of the liver with ensuing ischaemic necrosis of hepatocytes.", "PMID": 974529}
{"id": "pubmed23n0032_9922", "title": "Gastric emptying of solid meals in diabetics.", "content": "The gastric emptying rate of an isotopically labelled solid meal was compared in 29 insulin-dependent well-controlled diabetics and 18 normal controls. The diabetics were assessed for evidence of autonomic neuropathy. No significant difference in gastric emptying rate was found between controls and diabetics with or without autonomic neuropathy. Only three diabetics had greatly delayed gastric emptying, but in one of these the test had given a normal result on an earlier occasion.", "contents": "Gastric emptying of solid meals in diabetics. The gastric emptying rate of an isotopically labelled solid meal was compared in 29 insulin-dependent well-controlled diabetics and 18 normal controls. The diabetics were assessed for evidence of autonomic neuropathy. No significant difference in gastric emptying rate was found between controls and diabetics with or without autonomic neuropathy. Only three diabetics had greatly delayed gastric emptying, but in one of these the test had given a normal result on an earlier occasion.", "PMID": 974530}
{"id": "pubmed23n0032_9923", "title": "The 14C-glycocholate test in diabetic diarrhoea.", "content": "Twenty-four insulin-dependent diabetics, including seven with diabetic diarrhoea, were studied by means of the 14C-glycocholate (14C-GCA) test and various tests for autonomic dysfunction. The breath component of het test was abnormal in four of the seven patients with diarrhoea and one of the other diabetics. Three patients with diarrhoea and a positive breath test result responded to antibiotics, whereas two with diarrhoea and a negative test result did not. High faecal 14C, suggesting bile acid malabsorption, was found in only one patient with diarrhoea and he had previously failed to respond to cholestyramine. These results suggest that bacterial overgrowth in the small intestine does occur in some but not all patients with diabetic diarrhoea and that the 14C-GCA test can predict the response to antibiotics. All the patients with diabetic diarrhoea had good evidence of autonomic dysfunction.", "contents": "The 14C-glycocholate test in diabetic diarrhoea. Twenty-four insulin-dependent diabetics, including seven with diabetic diarrhoea, were studied by means of the 14C-glycocholate (14C-GCA) test and various tests for autonomic dysfunction. The breath component of het test was abnormal in four of the seven patients with diarrhoea and one of the other diabetics. Three patients with diarrhoea and a positive breath test result responded to antibiotics, whereas two with diarrhoea and a negative test result did not. High faecal 14C, suggesting bile acid malabsorption, was found in only one patient with diarrhoea and he had previously failed to respond to cholestyramine. These results suggest that bacterial overgrowth in the small intestine does occur in some but not all patients with diabetic diarrhoea and that the 14C-GCA test can predict the response to antibiotics. All the patients with diabetic diarrhoea had good evidence of autonomic dysfunction.", "PMID": 974531}
{"id": "pubmed23n0032_9924", "title": "Haemophilia A and the blood transfusion service: a Scottish study.", "content": "The demand for blood products containing factor VIII for treating patients with haemophilia A in south-east Scotland was reviewed. From 1961 to 1975 the demand for fresh frozen plasma (FFP), cryoprecipitate (CP), and antihaemophilic factor (AHF) increased by seven and a half times, while total donations increased by only a third. Patients with severe haemophilia A treated at the regional haemophilia centre used about 85% of the factor VIII issued in 1971-4, most of which was used on demand. A patient with severe haemophilia A on unlimited ondemand home treatment would need about 500 units of factor VII/kg body weight/year, and a regional haemophilia centre, treating moderate and mild cases as well as severe ones, would use 15000 units/patient/year. Altogether about 50 million units of factor VIII will be needed each year in the UK. Although cryoprecipitate is much harder to store and administer than AHF, its yield from plasma may be far greater and its cost far smaller. Unless the blood transfusion services receive increased amounts of money and reappraise their functions and  operation, it seems likely that they will have to rely increasingly on commercial (and costly) sources for the major plasma fractions.", "contents": "Haemophilia A and the blood transfusion service: a Scottish study. The demand for blood products containing factor VIII for treating patients with haemophilia A in south-east Scotland was reviewed. From 1961 to 1975 the demand for fresh frozen plasma (FFP), cryoprecipitate (CP), and antihaemophilic factor (AHF) increased by seven and a half times, while total donations increased by only a third. Patients with severe haemophilia A treated at the regional haemophilia centre used about 85% of the factor VIII issued in 1971-4, most of which was used on demand. A patient with severe haemophilia A on unlimited ondemand home treatment would need about 500 units of factor VII/kg body weight/year, and a regional haemophilia centre, treating moderate and mild cases as well as severe ones, would use 15000 units/patient/year. Altogether about 50 million units of factor VIII will be needed each year in the UK. Although cryoprecipitate is much harder to store and administer than AHF, its yield from plasma may be far greater and its cost far smaller. Unless the blood transfusion services receive increased amounts of money and reappraise their functions and  operation, it seems likely that they will have to rely increasingly on commercial (and costly) sources for the major plasma fractions.", "PMID": 974537}
{"id": "pubmed23n0032_9925", "title": "Deviation from prescribed drug treatment after discharge from hospital.", "content": "A study of 130 patients discharged from four hospital wards dealing mainly with acute medical cases showed that 66 deviated from the drug regimen prescribed on discharge. Of the patients, 46 did not have a clear understanding of the regimen (non-comprehension) and 20 of the remaining 84 patients understood the prescribed regimen but did not follow the instructions (non-compliance). The prescribing of complex drug regimens, and the availability of medicines prescribed before admission to hospital appeared to be the two main factors influencing non-comprehension and non-compliance.", "contents": "Deviation from prescribed drug treatment after discharge from hospital. A study of 130 patients discharged from four hospital wards dealing mainly with acute medical cases showed that 66 deviated from the drug regimen prescribed on discharge. Of the patients, 46 did not have a clear understanding of the regimen (non-comprehension) and 20 of the remaining 84 patients understood the prescribed regimen but did not follow the instructions (non-compliance). The prescribing of complex drug regimens, and the availability of medicines prescribed before admission to hospital appeared to be the two main factors influencing non-comprehension and non-compliance.", "PMID": 974539}
{"id": "pubmed23n0032_9926", "title": "Control of distension of varicose veins achieved by leg bandages, as used after injection sclerotherapy.", "content": "A study was performed to determine whether the pressures routinely produced by bandaging for compression sclerotherapy of varicose veins are adequate to maintain the superfical veins almost empty of blood. The results suggest that well-applied bandages can provide sufficient support to combat the high distending pressures found in varicose veins. The large variation among different surgeons, however, indicates that any clinical assessment of compression sclerotherapy should include measurement of the pressure at which the bandages are applied.", "contents": "Control of distension of varicose veins achieved by leg bandages, as used after injection sclerotherapy. A study was performed to determine whether the pressures routinely produced by bandaging for compression sclerotherapy of varicose veins are adequate to maintain the superfical veins almost empty of blood. The results suggest that well-applied bandages can provide sufficient support to combat the high distending pressures found in varicose veins. The large variation among different surgeons, however, indicates that any clinical assessment of compression sclerotherapy should include measurement of the pressure at which the bandages are applied.", "PMID": 974569}
{"id": "pubmed23n0032_9927", "title": "Effect of ethyloestrenol on fibrinolysis in the vessel wall.", "content": "Forty-nine patients with decreased fibrinolytic activity in the vessel walls or a decreased release mechanism, or both, were treated with ethyloestrenol for three to 17 months. Forty-five of the patients had had recurrent, phlebographically verified, deep venous thrombosis (DVT) and four had arterial thrombosis. Ethyloestrenol 8 mg/day was given to 31 patients and 4 mg/day was given to 12. The remaining six patients had been treated with a combination of phenformin and ethloestrenol. The phenformin was withdrawn but they were kept on ethyloestrenol 8 mg/day. Another 15 patients with a normal fibrinolytic system--four with recurrent DVT and 11 with severe arteriosclerosis--were given ethyloestrenol 8 mg/day. The spontaneous fibrinolytic activity, local fibrinolytic activity during standardised venous occlusion of the arms, and fibrinolytic activity of the vessel walls increased significantly after treatment with ethyloestrenol 8 mg/day for three months. No further increase occurred after three months, and ethyloestrenol 4 mg/day had no effect. No values rose significantly in the patients with a normal fibrinolytic system. One patient suffered a recurrence within three months of treatment, before the fibrinolytic system became normal. In one patient the fibrinolytic defect reappeared after 10 months in spite of continued treatment. Two of the three women of fertile age developed irregular cycles and intermenstrual bleeding, which disappeared when the treatment was withdrawn. No other side effects were observed.", "contents": "Effect of ethyloestrenol on fibrinolysis in the vessel wall. Forty-nine patients with decreased fibrinolytic activity in the vessel walls or a decreased release mechanism, or both, were treated with ethyloestrenol for three to 17 months. Forty-five of the patients had had recurrent, phlebographically verified, deep venous thrombosis (DVT) and four had arterial thrombosis. Ethyloestrenol 8 mg/day was given to 31 patients and 4 mg/day was given to 12. The remaining six patients had been treated with a combination of phenformin and ethloestrenol. The phenformin was withdrawn but they were kept on ethyloestrenol 8 mg/day. Another 15 patients with a normal fibrinolytic system--four with recurrent DVT and 11 with severe arteriosclerosis--were given ethyloestrenol 8 mg/day. The spontaneous fibrinolytic activity, local fibrinolytic activity during standardised venous occlusion of the arms, and fibrinolytic activity of the vessel walls increased significantly after treatment with ethyloestrenol 8 mg/day for three months. No further increase occurred after three months, and ethyloestrenol 4 mg/day had no effect. No values rose significantly in the patients with a normal fibrinolytic system. One patient suffered a recurrence within three months of treatment, before the fibrinolytic system became normal. In one patient the fibrinolytic defect reappeared after 10 months in spite of continued treatment. Two of the three women of fertile age developed irregular cycles and intermenstrual bleeding, which disappeared when the treatment was withdrawn. No other side effects were observed.", "PMID": 974570}
{"id": "pubmed23n0032_9928", "title": "Surgical audit: one year's experience in a teaching hospital.", "content": "For 12 months the surgical staff at Hope Hospital have operated a form of audit. The monitored information included work load, methods of treatment, complications, misdiagnoses, and deaths. The method described is suitable for any district general or teaching hospital provided adequate secretarial help is available. In addition to helping to maintain standards, an audit of this type has a positive educational role.", "contents": "Surgical audit: one year's experience in a teaching hospital. For 12 months the surgical staff at Hope Hospital have operated a form of audit. The monitored information included work load, methods of treatment, complications, misdiagnoses, and deaths. The method described is suitable for any district general or teaching hospital provided adequate secretarial help is available. In addition to helping to maintain standards, an audit of this type has a positive educational role.", "PMID": 974581}
{"id": "pubmed23n0032_9929", "title": "The curability of breast cancer.", "content": "The age-correlated survival rates for 982 women treated for localised breast cancer were analysed retrospectively. Analysis by size of the primary cancer showed that those with smaller cancers had a significantly higher survival rate and a significantly reduced probability of dying of cancer. Cured groups of patients were identified for all but those with the smallest tumours--2.0 cm in diameter or smaller. A long period of follow-up (to 25 years) is necessary to establish the presence of a large cured group in such cases.", "contents": "The curability of breast cancer. The age-correlated survival rates for 982 women treated for localised breast cancer were analysed retrospectively. Analysis by size of the primary cancer showed that those with smaller cancers had a significantly higher survival rate and a significantly reduced probability of dying of cancer. Cured groups of patients were identified for all but those with the smallest tumours--2.0 cm in diameter or smaller. A long period of follow-up (to 25 years) is necessary to establish the presence of a large cured group in such cases.", "PMID": 974608}
{"id": "pubmed23n0032_9930", "title": "Hormonal profiles after the menopause.", "content": "The endocrinological changes of the climacteric have been defined by studying the concentrations of follicle-stimulating hormone (FSH), luteinising hormone (LH), androstenedione, testosterone, oestrone, and oestradiol in 60 normal postmenopausal women of different menopausal ages. The women were studied in six groups, according to the number of years since their menopause. One year after the menopause androstenedione, oestrone, and oestradiol concentrations were reduced to about 20% of the values recorded during the early proliferative phase of the menstrual cycle. At the same time the mean concentration of FSH had risen by a factor of 13-4 and that of LH by a factor of 3-0. Concentrations of both gonadotrophins reached a peak of 18-4 and 3-4 times the proliferative phase value respectively after two to three years, and then gradually declined in the next three decades to values that were 40-50% of these maximal levels. Testosterone concentrations remained mostly in the normal range for premenopausal women but were depressed to 60% of these levels two to five years after the menopause, and the mean androstenedione levels showed a significant increase in the same group of women. The concentrations of both oestrone and oestradiol remained consistently low for 10 years after the menopause, but oestradiol concentrations inexplicably increased in the last two decades, with levels at the lower end of normal range for reproductive women in six patients.", "contents": "Hormonal profiles after the menopause. The endocrinological changes of the climacteric have been defined by studying the concentrations of follicle-stimulating hormone (FSH), luteinising hormone (LH), androstenedione, testosterone, oestrone, and oestradiol in 60 normal postmenopausal women of different menopausal ages. The women were studied in six groups, according to the number of years since their menopause. One year after the menopause androstenedione, oestrone, and oestradiol concentrations were reduced to about 20% of the values recorded during the early proliferative phase of the menstrual cycle. At the same time the mean concentration of FSH had risen by a factor of 13-4 and that of LH by a factor of 3-0. Concentrations of both gonadotrophins reached a peak of 18-4 and 3-4 times the proliferative phase value respectively after two to three years, and then gradually declined in the next three decades to values that were 40-50% of these maximal levels. Testosterone concentrations remained mostly in the normal range for premenopausal women but were depressed to 60% of these levels two to five years after the menopause, and the mean androstenedione levels showed a significant increase in the same group of women. The concentrations of both oestrone and oestradiol remained consistently low for 10 years after the menopause, but oestradiol concentrations inexplicably increased in the last two decades, with levels at the lower end of normal range for reproductive women in six patients.", "PMID": 974609}
{"id": "pubmed23n0032_9931", "title": "Observations on electrocardiogram and plasma catecholamines during dental procedures: the forgotten vagus.", "content": "Emotional stress is conventionally considered to be associated with tachycardia and enhanced sympathetic activity. The electrocardiogram and plasma catecholamine and lipid concentrations were observed in 21 young healthy women undergoing dental procedures. Ten of these received premedication with the beta-blocking agent oxprenolol and 11 with a placebo, administered on a double-blind randomised basis. Mild tachycardia occurred in the placebo group a few minutes before and a few minutes after dentistry, but there was a reduction in heart rate immediately before and during the procedure. The pattern was similar in the group who received oxprenolol, though the heart rates at each stage were lower. Plasma adrenaline concentrations were much higher in the samples taken during the procedure than in those taken shortly before and after it. Plasma noradrenaline and lipid concentrations remained unchanged. A decrease in heart rate in the face of intense emotional arousal and an increased plasma adrenaline concentration suggest that the expectation or experience of pain may be associated with parasympathetic dominance despite greatly enhanced sympathetic activity.", "contents": "Observations on electrocardiogram and plasma catecholamines during dental procedures: the forgotten vagus. Emotional stress is conventionally considered to be associated with tachycardia and enhanced sympathetic activity. The electrocardiogram and plasma catecholamine and lipid concentrations were observed in 21 young healthy women undergoing dental procedures. Ten of these received premedication with the beta-blocking agent oxprenolol and 11 with a placebo, administered on a double-blind randomised basis. Mild tachycardia occurred in the placebo group a few minutes before and a few minutes after dentistry, but there was a reduction in heart rate immediately before and during the procedure. The pattern was similar in the group who received oxprenolol, though the heart rates at each stage were lower. Plasma adrenaline concentrations were much higher in the samples taken during the procedure than in those taken shortly before and after it. Plasma noradrenaline and lipid concentrations remained unchanged. A decrease in heart rate in the face of intense emotional arousal and an increased plasma adrenaline concentration suggest that the expectation or experience of pain may be associated with parasympathetic dominance despite greatly enhanced sympathetic activity.", "PMID": 974610}
{"id": "pubmed23n0032_9932", "title": "Meconium ileus equivalent in adults with cystic fibrosis of pancreas: a report of six cases.", "content": "Eleven episodes of \"meconium ileus equivalent\" have been seen in six adults with cystic fibrosis of the pancreas. Three patients were initially treated surgically; one died and the other two developed serious postoperative chest infections. Six episodes were successfully treated medically with acetylcysteine orally and by enema, nasogastric suction, and intravenous fluids. Operation should be avoided if possible, and maintenance treatment with acetylcysteine may be necessary to prevent relapse.", "contents": "Meconium ileus equivalent in adults with cystic fibrosis of pancreas: a report of six cases. Eleven episodes of \"meconium ileus equivalent\" have been seen in six adults with cystic fibrosis of the pancreas. Three patients were initially treated surgically; one died and the other two developed serious postoperative chest infections. Six episodes were successfully treated medically with acetylcysteine orally and by enema, nasogastric suction, and intravenous fluids. Operation should be avoided if possible, and maintenance treatment with acetylcysteine may be necessary to prevent relapse.", "PMID": 974611}
{"id": "pubmed23n0032_9933", "title": "General practice observed. Child abuse and general practice.", "content": "In a general practice of 9250 patients with 1841 children under 10 there were 12 cases of actual abuse during 1973-6. In March 1976 30 children were at risk. A preventive scheme was set up and the short-term outcome was good. There were no cases of serious abuse among the children at risk.", "contents": "General practice observed. Child abuse and general practice. In a general practice of 9250 patients with 1841 children under 10 there were 12 cases of actual abuse during 1973-6. In March 1976 30 children were at risk. A preventive scheme was set up and the short-term outcome was good. There were no cases of serious abuse among the children at risk.", "PMID": 974619}
{"id": "pubmed23n0032_9934", "title": "Distribution of nephrological services for adults in Great Britain. Report of the Executive Committee of the Renal Association.", "content": "A survey was performed to find out how many doctors in Great Britain were providing nephrological services. The number of such doctors in each region correlated closely with the size of population, but the relation between the numbers of sessions they provided and the size of population, though significant, was not nearly so close. The number of sessions provided correlated strongly with both the number of patients on dialysis and the number of outpatients seen each week. These findings indicated that nephrological services were unevenly distributed throughout the country, while patients with renal diseases are probably evenly distributed. Even in the regions providing the most sessions demand still exceeds supply. In Britain only 62-0 patients per million population were being treated for terminal renal failure at 31 December 1975, whereas over twice that number were being treated in Switzerland (136-1 per million) and Denmark (132-4 per million). Despite the deficiencies in the service, doctors attempt to see all patients with a renal disorder at least once and to treat acute renal failure, though many patients cannot be followed up.", "contents": "Distribution of nephrological services for adults in Great Britain. Report of the Executive Committee of the Renal Association. A survey was performed to find out how many doctors in Great Britain were providing nephrological services. The number of such doctors in each region correlated closely with the size of population, but the relation between the numbers of sessions they provided and the size of population, though significant, was not nearly so close. The number of sessions provided correlated strongly with both the number of patients on dialysis and the number of outpatients seen each week. These findings indicated that nephrological services were unevenly distributed throughout the country, while patients with renal diseases are probably evenly distributed. Even in the regions providing the most sessions demand still exceeds supply. In Britain only 62-0 patients per million population were being treated for terminal renal failure at 31 December 1975, whereas over twice that number were being treated in Switzerland (136-1 per million) and Denmark (132-4 per million). Despite the deficiencies in the service, doctors attempt to see all patients with a renal disorder at least once and to treat acute renal failure, though many patients cannot be followed up.", "PMID": 974655}
{"id": "pubmed23n0032_9935", "title": "Titanium-strip cranioplasty.", "content": "Titanium strips have been used to repair skull defects when preformed metal plates are not avilable and simple wiring would not be enough. The technique is simple, obviates the need to dissect the dura mater, and gives greater resistance to further impact injury than an inlay technique.", "contents": "Titanium-strip cranioplasty. Titanium strips have been used to repair skull defects when preformed metal plates are not avilable and simple wiring would not be enough. The technique is simple, obviates the need to dissect the dura mater, and gives greater resistance to further impact injury than an inlay technique.", "PMID": 974656}
{"id": "pubmed23n0032_9936", "title": "Treatment of candidal urinary tract infection with nifuratel.", "content": "Three patients with candidal urinary tract infections were successfully treated with oral nifuratel, a nitrofuran antimicrobial agent active against yeast and Trichomonas as well as urinary bacterial pathogens. The recommended dose is 400 mg thrice daily for a week. No side effects that could be attributed to the treatment were noted. Minimum ibhibitory concentration determinations for nifuratel against Candida strains of five species showed that 48 out of 59 organisms were inhibited by 50 mg/1 or less, the three strains of Candida species eliminated from our treated patients having MICs of nifuratel in the range of 10-50 mg/1.", "contents": "Treatment of candidal urinary tract infection with nifuratel. Three patients with candidal urinary tract infections were successfully treated with oral nifuratel, a nitrofuran antimicrobial agent active against yeast and Trichomonas as well as urinary bacterial pathogens. The recommended dose is 400 mg thrice daily for a week. No side effects that could be attributed to the treatment were noted. Minimum ibhibitory concentration determinations for nifuratel against Candida strains of five species showed that 48 out of 59 organisms were inhibited by 50 mg/1 or less, the three strains of Candida species eliminated from our treated patients having MICs of nifuratel in the range of 10-50 mg/1.", "PMID": 974657}
{"id": "pubmed23n0032_9937", "title": "Preoperative prediction of postoperative deep vein thrombosis.", "content": "A range of clinical data was obtained from 124 patients about to undergo operation and several coagulation tests were performed. No patient received prophylaxis for deep vein thrombosis, and isotopic scanning after operation showed that 20 patients had developed thrombosis. a simiple prognostic index for predicting which patients would develop postoperative deep vein thrombosis was constructed using the clinical and coagulation data obtained before operation. The five variables with the best predictive power-euglobulin lysis time, age, presence of varicose veins, fibrin related antigen, and percentage overweight-produced an equation that identfied 95% of those who developed deep vein thrombosis and misallocated only 28% of those who did not develop thrombosis. In view of the complications that low-dose heparin and dextran can cause, giving prophylaxis to under a third of the patients who will not develop deep vein thrombosis is clearly better than giving it to all.", "contents": "Preoperative prediction of postoperative deep vein thrombosis. A range of clinical data was obtained from 124 patients about to undergo operation and several coagulation tests were performed. No patient received prophylaxis for deep vein thrombosis, and isotopic scanning after operation showed that 20 patients had developed thrombosis. a simiple prognostic index for predicting which patients would develop postoperative deep vein thrombosis was constructed using the clinical and coagulation data obtained before operation. The five variables with the best predictive power-euglobulin lysis time, age, presence of varicose veins, fibrin related antigen, and percentage overweight-produced an equation that identfied 95% of those who developed deep vein thrombosis and misallocated only 28% of those who did not develop thrombosis. In view of the complications that low-dose heparin and dextran can cause, giving prophylaxis to under a third of the patients who will not develop deep vein thrombosis is clearly better than giving it to all.", "PMID": 974658}
{"id": "pubmed23n0032_9938", "title": "Virus-like particles in paraspinal muscle in scoliosis.", "content": "Biopsy material from the skeletal muscle (paraxials) of 21 patients with scoliosis was examined by light and electron microscopy. Virus-like particles, 17 nm in diameter with a crystalline structure, were identified in the skeletal muscle fibres of four patients. Associated changes in the sarcoplasm included swelling of mitochondria, presence of lipid droplets, and vesicular structures. Serological studies and culture for virus isolation gave negative results. An excess of lipid (predominantly in type 1 fibres) was noted in the skeletal muscle of several other cases. The significance of these findings is obscure, but the morphology of the paraxial muscles of patients with scoliosis and controls is currently being investigated in greater detail.", "contents": "Virus-like particles in paraspinal muscle in scoliosis. Biopsy material from the skeletal muscle (paraxials) of 21 patients with scoliosis was examined by light and electron microscopy. Virus-like particles, 17 nm in diameter with a crystalline structure, were identified in the skeletal muscle fibres of four patients. Associated changes in the sarcoplasm included swelling of mitochondria, presence of lipid droplets, and vesicular structures. Serological studies and culture for virus isolation gave negative results. An excess of lipid (predominantly in type 1 fibres) was noted in the skeletal muscle of several other cases. The significance of these findings is obscure, but the morphology of the paraxial muscles of patients with scoliosis and controls is currently being investigated in greater detail.", "PMID": 974659}
{"id": "pubmed23n0032_9939", "title": "Muscle changes in acromegaly.", "content": "Muscle biopsy specimens were obtained from 18 patients with acromegaly of varying degrees of severity. Half the specimens showed hypertrophy of type 1 fibres, while atrophy was most often seen in type 2 fibres. A direct correlation between muscle appearances and growth hormone levels was not observed. The needle biopsy technique is particularly suitable for determining the natural history of acrometalic myopathy.", "contents": "Muscle changes in acromegaly. Muscle biopsy specimens were obtained from 18 patients with acromegaly of varying degrees of severity. Half the specimens showed hypertrophy of type 1 fibres, while atrophy was most often seen in type 2 fibres. A direct correlation between muscle appearances and growth hormone levels was not observed. The needle biopsy technique is particularly suitable for determining the natural history of acrometalic myopathy.", "PMID": 974660}
{"id": "pubmed23n0032_9940", "title": "A predischarge unit in a long-stay hospital for subnormality.", "content": "A newly built residential ward in the grounds of a long-stay mental subnormality hospital was converted into a predischarge unit to cater for those patients who could probably be discharged after some special training. So far 31 residents have been trained over the past two years; 18 (58%) have been discharged to community residences, and five were waiting for discharge. The high success rate shows the value and usefulness of a predischage unit in a long-stay hospital, and also shows that a purpose-built building is not necessary so long as the staff have the initiative and will to succeed.", "contents": "A predischarge unit in a long-stay hospital for subnormality. A newly built residential ward in the grounds of a long-stay mental subnormality hospital was converted into a predischarge unit to cater for those patients who could probably be discharged after some special training. So far 31 residents have been trained over the past two years; 18 (58%) have been discharged to community residences, and five were waiting for discharge. The high success rate shows the value and usefulness of a predischage unit in a long-stay hospital, and also shows that a purpose-built building is not necessary so long as the staff have the initiative and will to succeed.", "PMID": 974666}
{"id": "pubmed23n0032_9941", "title": "Barbiturate prescribing: psychiatrists' views.", "content": "When questioned by post most of a one-in five sample of consultant psychiatrists replied that they did not favour the use of barbiturates as hypnotics or a sedatives.", "contents": "Barbiturate prescribing: psychiatrists' views. When questioned by post most of a one-in five sample of consultant psychiatrists replied that they did not favour the use of barbiturates as hypnotics or a sedatives.", "PMID": 974668}
{"id": "pubmed23n0032_9942", "title": "Contribution of preterm delivery to perinatal mortality.", "content": "A detailed retrospective analysis was made of the records of 486 preterm infants, who accounted for 5-1% of all births during 1973 and 1974. Whereas preterm delivery did not contribute to perinatal mortality in terms of stillbirth, it outweighed all other causes in terms of early neonatal deaths. Preterm birth was responsible for 85% of the early neonatal deaths not due to lethal congenital deformities. Early neonatal mortality rates were closely linked both to gestational age and birth weight and to the reason for preterm birth. Early neonatal mortality was high (97 per 1000) when preterm labour was spontaneous, whether or not associated with material or fetal disease or with multiple pregnancy, but low (27 per 1000) when preterm delivery was elective. Preventing spontaneous preterm labour would considerably reduce neonatal mortality in our community.", "contents": "Contribution of preterm delivery to perinatal mortality. A detailed retrospective analysis was made of the records of 486 preterm infants, who accounted for 5-1% of all births during 1973 and 1974. Whereas preterm delivery did not contribute to perinatal mortality in terms of stillbirth, it outweighed all other causes in terms of early neonatal deaths. Preterm birth was responsible for 85% of the early neonatal deaths not due to lethal congenital deformities. Early neonatal mortality rates were closely linked both to gestational age and birth weight and to the reason for preterm birth. Early neonatal mortality was high (97 per 1000) when preterm labour was spontaneous, whether or not associated with material or fetal disease or with multiple pregnancy, but low (27 per 1000) when preterm delivery was elective. Preventing spontaneous preterm labour would considerably reduce neonatal mortality in our community.", "PMID": 974709}
{"id": "pubmed23n0032_9943", "title": "Can phenformin-induced lactic acidosis be prevented?", "content": "Although patients taking phenformin are more likely to develop lactic acidosis in the presence of renal, cardiovascular, or hepatic disease, criteria for safe use of the drug are not well established. Eight diabetics died of lactic acidosis in Nottingham in 1972-5 and all were taking phenformin in therapeutic doses. Six had attended the diabetic clinic within a month of their terminal illness. Two patients had appreciable renal impairment and should not have been given phenformin. Four had hypertension and minimal evidence of renal disease, while in two no predisposing factor was identified. There are so many contraindications to the use of phenformin that it is doubtful whether patients on the drug can be monitored adequately. We suggest that phenformin should be withdrawn from general use.", "contents": "Can phenformin-induced lactic acidosis be prevented? Although patients taking phenformin are more likely to develop lactic acidosis in the presence of renal, cardiovascular, or hepatic disease, criteria for safe use of the drug are not well established. Eight diabetics died of lactic acidosis in Nottingham in 1972-5 and all were taking phenformin in therapeutic doses. Six had attended the diabetic clinic within a month of their terminal illness. Two patients had appreciable renal impairment and should not have been given phenformin. Four had hypertension and minimal evidence of renal disease, while in two no predisposing factor was identified. There are so many contraindications to the use of phenformin that it is doubtful whether patients on the drug can be monitored adequately. We suggest that phenformin should be withdrawn from general use.", "PMID": 974710}
{"id": "pubmed23n0032_9944", "title": "Visual and nonvisual units recorded from the optic tectum of Gallus domesticus.", "content": "Units recorded from the optic tectum of Gallus domesticus were tested for responses to visual, auditory and somesthetic and stimuli. Units recorded from the superficial tectal layers (stratum opticum, stratum griseum et fibrosum superficiale) responded only to visual stimuli. On the basis of the unit's responsiveness to the directionality of a moving visual stimulus, three unit types referred to as pandirectional, unidirectional, and multidirectional units were identified. In the deeper layers (stratum griseum centrale, stratum album centrale) unimodal and bimodal units were encountered. These units responded to visual, auditory, and/or somesthetic stimuli. These results indicate that the tectal cortex of Gallus domesticus is subdivided into two functional divisions: the superficial layers which correlate visual information and the deep layers which correlate visual and nonvisual information.", "contents": "Visual and nonvisual units recorded from the optic tectum of Gallus domesticus. Units recorded from the optic tectum of Gallus domesticus were tested for responses to visual, auditory and somesthetic and stimuli. Units recorded from the superficial tectal layers (stratum opticum, stratum griseum et fibrosum superficiale) responded only to visual stimuli. On the basis of the unit's responsiveness to the directionality of a moving visual stimulus, three unit types referred to as pandirectional, unidirectional, and multidirectional units were identified. In the deeper layers (stratum griseum centrale, stratum album centrale) unimodal and bimodal units were encountered. These units responded to visual, auditory, and/or somesthetic stimuli. These results indicate that the tectal cortex of Gallus domesticus is subdivided into two functional divisions: the superficial layers which correlate visual information and the deep layers which correlate visual and nonvisual information.", "PMID": 974720}
{"id": "pubmed23n0032_9945", "title": "Hyperemotionality and enhanced tonic immobility after septal lesions in the rabbit.", "content": "Large lesions of the septal region in the rabbit increased tonic immobility as measured by the number of successful inductions and by duration of immobility. Open field activity was also increased. Septally lesioned rabbits also exhibited increased emotionality, although the topography of the 'septal syndrome' in these rabbits was different from that described for rats. The septally lesioned rabbits exhibited increases in fear reactions such as thumping, escape responses and vocalization when caught, rather than increased aggressiveness. The results are discussed in terms of loss of inhibition of species-typical responses after septal damage.", "contents": "Hyperemotionality and enhanced tonic immobility after septal lesions in the rabbit. Large lesions of the septal region in the rabbit increased tonic immobility as measured by the number of successful inductions and by duration of immobility. Open field activity was also increased. Septally lesioned rabbits also exhibited increased emotionality, although the topography of the 'septal syndrome' in these rabbits was different from that described for rats. The septally lesioned rabbits exhibited increases in fear reactions such as thumping, escape responses and vocalization when caught, rather than increased aggressiveness. The results are discussed in terms of loss of inhibition of species-typical responses after septal damage.", "PMID": 974721}
{"id": "pubmed23n0032_9946", "title": "Retinal projections in cyprinid fishes: a degeneration and radioautographic study.", "content": "The retinal projections of three species of cyprinid fish (Cyprinus macrolepidotus, Cyprinus carpio, Rutilus rutilus) were examined with Nauta and Fink-Heimer methods following enucleation and with radioautography following intraocular injection of [3H]-L-proline. Optic tract axons cross completely in the optic chiasma and are distributed to the hypothalamus (nucleus opticus hypothalamicus pars magnocellularis), the thalamo-pretectal region (11 distinct primary optic centers), and the tectum opticum (stratum fibrosum et griseum superficiale, stratum griseum centrale and stratum album centrale). No accessory optic tract was found. The experimental studies on visual projection of various teleosts are reviewed and discussed.", "contents": "Retinal projections in cyprinid fishes: a degeneration and radioautographic study. The retinal projections of three species of cyprinid fish (Cyprinus macrolepidotus, Cyprinus carpio, Rutilus rutilus) were examined with Nauta and Fink-Heimer methods following enucleation and with radioautography following intraocular injection of [3H]-L-proline. Optic tract axons cross completely in the optic chiasma and are distributed to the hypothalamus (nucleus opticus hypothalamicus pars magnocellularis), the thalamo-pretectal region (11 distinct primary optic centers), and the tectum opticum (stratum fibrosum et griseum superficiale, stratum griseum centrale and stratum album centrale). No accessory optic tract was found. The experimental studies on visual projection of various teleosts are reviewed and discussed.", "PMID": 974722}
{"id": "pubmed23n0032_9947", "title": "Vestibular projections to the thalamus of the pigeon.", "content": "Microelectrode recording in the thalamus of pigeons subjected to tilt and sinusoidal rotational stimuli around the vertical, longitudinal and transversal axes revealed vestibularly driven units in two thalamic nuclei, the nucleus posteroventralis and the nucleus principalis precommissuralis. Many of these units responded in a complex manner suggesting that inputs from contralateral and ipsilateral cupulae and maculae converged on them. A few units received additional visual or proprioceptive information. The homology relationship with a mammalian vestibular thalamic nucleus is discussed briefly.", "contents": "Vestibular projections to the thalamus of the pigeon. Microelectrode recording in the thalamus of pigeons subjected to tilt and sinusoidal rotational stimuli around the vertical, longitudinal and transversal axes revealed vestibularly driven units in two thalamic nuclei, the nucleus posteroventralis and the nucleus principalis precommissuralis. Many of these units responded in a complex manner suggesting that inputs from contralateral and ipsilateral cupulae and maculae converged on them. A few units received additional visual or proprioceptive information. The homology relationship with a mammalian vestibular thalamic nucleus is discussed briefly.", "PMID": 974723}
{"id": "pubmed23n0032_9948", "title": "An electron microscopic study of lesion-induced synaptogenesis in the dentate gyrus of the adult rat. I. Magnitude and time course of degeneration.", "content": "Synapses in the rat dentate gyrus are rapidly lost after removal of the primary input from the entorhinal cortex. In this paper we describe the extent and time course of degeneration and in the subsequent paper the nature of the reinnervation processes. They synapses of entorhinal afferents are remarkably concentrated in their zone of termination. Unilateral removal of the rat entorhinal cortex results in the loss of about 86% of all synapses in the outer three-fourths of the molecular layer of the epsilateral dentate gyrus. Entorhinal synapses are all asymmetric (Gray type I) and terminate on dendritic spines. Analysis of the degeneration reaction provides a means to examine the characteristics of the loss of a relatively homogeneous afferent on a single cell type. The morphological characteristics of the the degenerating terminals showed some heterogeneity; both the electron lucent and electron dense types of degenerating terminals were identified. The electron lucent type was observed only at short survival times. The time course of the loss of degenerating terminals was resolvable into two components, each of which followed first order decay kinetics. Thus degenerating entorhinal terminals behaved as a population which disappeared randomly at a rate dependent on the fraction of terminals present at any time. The loss of degenerating terminals was accompanied by the loss of postsynaptic sites. At short survival times the majority of postsynaptic sites (defined by the presence of a postsynaptic density) had disappeared. There was also a loss of complex spines and some shrinkage of the molecular layer.", "contents": "An electron microscopic study of lesion-induced synaptogenesis in the dentate gyrus of the adult rat. I. Magnitude and time course of degeneration. Synapses in the rat dentate gyrus are rapidly lost after removal of the primary input from the entorhinal cortex. In this paper we describe the extent and time course of degeneration and in the subsequent paper the nature of the reinnervation processes. They synapses of entorhinal afferents are remarkably concentrated in their zone of termination. Unilateral removal of the rat entorhinal cortex results in the loss of about 86% of all synapses in the outer three-fourths of the molecular layer of the epsilateral dentate gyrus. Entorhinal synapses are all asymmetric (Gray type I) and terminate on dendritic spines. Analysis of the degeneration reaction provides a means to examine the characteristics of the loss of a relatively homogeneous afferent on a single cell type. The morphological characteristics of the the degenerating terminals showed some heterogeneity; both the electron lucent and electron dense types of degenerating terminals were identified. The electron lucent type was observed only at short survival times. The time course of the loss of degenerating terminals was resolvable into two components, each of which followed first order decay kinetics. Thus degenerating entorhinal terminals behaved as a population which disappeared randomly at a rate dependent on the fraction of terminals present at any time. The loss of degenerating terminals was accompanied by the loss of postsynaptic sites. At short survival times the majority of postsynaptic sites (defined by the presence of a postsynaptic density) had disappeared. There was also a loss of complex spines and some shrinkage of the molecular layer.", "PMID": 974734}
{"id": "pubmed23n0032_9949", "title": "The mechanism of inhibition of neuronal activity by opiates in the spinal cord of cat.", "content": "Extra- and intracellular recordings from motoneurones, interneurones and dorsal horn neurones (laminae 4 and 5) were obtained from the lumbar segments (L6-L7) of spinalised (Th 9/10) or pentobarbital-anaesthetised and anaemically decorticated cats. In the majority of spinal neurones microelectrophoretically applied morphine and levorphanol reversibly depressed spontaneous as well as stimulus-evoked and L-glutamate- or acetylcholine-induced activity. There is evidence that opiates block L-glutamate-induced depolarisations by impairing the Na+-influx triggered at the postsynaptic membrane. These depressant effects of opiates could be antagonised by naloxone, and, except in a few cases, were not associated with hyperpolarisation of the cell. Dextrorphan, the D+ enantiomer of levorphanol, displayed no such depressant actions, indicating that stereospecific receptors mediate the depressant effects of opiates. Phoretically applied atropine, procaine and Ca2+ ions have anti-glutamate and anti-acetylcholine actions similar to opiates, but these actions were not antagonised by naloxone. The hyperpolarising effect of glycine was not influenced at dose levels of opiates sufficient to suppress depolarisation induced by L-glutamate or acetylcholine. Microelectrophoretically administered morphine and levorphanol slowed the rate of rise of mono- and polysynaptic EPSPs by a naloxone-antagonisable mechanism at dose levels where almost no alteration in spike shape was detectable. Increased doses of morphine and levorphanol reduced the amplitude of IPSPs and completely blocked or reduced the amplitude of both direct- and antidromically-evoked spikes. These effects of increased doses of opiates were not antagonised by naloxone. Intravenous injection of 2 mg/kg of morphine or 20 mug/kg of Fentanyl mimicked the suppression of spontaneous and evoked neuronal activity observed after phoretic administration. This depressant action of systemically applied opiates could be transiently antagonised by phoretic administration of naloxone. The results are discussed with respect to a stereospecific action of opiates at a postsynaptic receptive site in the spinal cord.", "contents": "The mechanism of inhibition of neuronal activity by opiates in the spinal cord of cat. Extra- and intracellular recordings from motoneurones, interneurones and dorsal horn neurones (laminae 4 and 5) were obtained from the lumbar segments (L6-L7) of spinalised (Th 9/10) or pentobarbital-anaesthetised and anaemically decorticated cats. In the majority of spinal neurones microelectrophoretically applied morphine and levorphanol reversibly depressed spontaneous as well as stimulus-evoked and L-glutamate- or acetylcholine-induced activity. There is evidence that opiates block L-glutamate-induced depolarisations by impairing the Na+-influx triggered at the postsynaptic membrane. These depressant effects of opiates could be antagonised by naloxone, and, except in a few cases, were not associated with hyperpolarisation of the cell. Dextrorphan, the D+ enantiomer of levorphanol, displayed no such depressant actions, indicating that stereospecific receptors mediate the depressant effects of opiates. Phoretically applied atropine, procaine and Ca2+ ions have anti-glutamate and anti-acetylcholine actions similar to opiates, but these actions were not antagonised by naloxone. The hyperpolarising effect of glycine was not influenced at dose levels of opiates sufficient to suppress depolarisation induced by L-glutamate or acetylcholine. Microelectrophoretically administered morphine and levorphanol slowed the rate of rise of mono- and polysynaptic EPSPs by a naloxone-antagonisable mechanism at dose levels where almost no alteration in spike shape was detectable. Increased doses of morphine and levorphanol reduced the amplitude of IPSPs and completely blocked or reduced the amplitude of both direct- and antidromically-evoked spikes. These effects of increased doses of opiates were not antagonised by naloxone. Intravenous injection of 2 mg/kg of morphine or 20 mug/kg of Fentanyl mimicked the suppression of spontaneous and evoked neuronal activity observed after phoretic administration. This depressant action of systemically applied opiates could be transiently antagonised by phoretic administration of naloxone. The results are discussed with respect to a stereospecific action of opiates at a postsynaptic receptive site in the spinal cord.", "PMID": 974735}
{"id": "pubmed23n0032_9950", "title": "The effect of antisynaptosomal plasma membrane antibodies on memory.", "content": "Antisynaptosomal plasma membrane antibodies were introduced through an infusion cannula into rat brain and their effects on behaviour were tested. Four different learning paradigms were used, two appetitively and two aversively motivated, to show impairment in memory retrieval. No effects were found on aquisition, motor activity, or motivation.", "contents": "The effect of antisynaptosomal plasma membrane antibodies on memory. Antisynaptosomal plasma membrane antibodies were introduced through an infusion cannula into rat brain and their effects on behaviour were tested. Four different learning paradigms were used, two appetitively and two aversively motivated, to show impairment in memory retrieval. No effects were found on aquisition, motor activity, or motivation.", "PMID": 974736}
{"id": "pubmed23n0032_9951", "title": "An electron microscopic study of lesion-induced synaptogenesis in the dentate gyrus of the adult rat. II. Reappearance of morphologically normal synaptic contacts.", "content": "Intact synapses in the denervated area of the rat dentate gyrus are reduced to 14% of those normally present 2-4 days following a unilateral entorhinal lesion. By 160-240 days after lesion, the former entorhinal terminal zone is repopulated with new synapses. In all, there is more than a 5-fold increase in the density of intact synapses in the denervated zone between 2 and 240 days post-lesion, and the denervated zone of the molecular layer is restored to 80% of control values. The synapses are Gray type I and are formed on simple and complex spines which closely resemble those normally present. A few boutons have an abnormally large number of synaptic junctions. Reinnervation seems to progress at differential rates. Synapses are rapidly regained up to 30 days after operation, but thereafter the reacquisition of synaptic connections is much slower. Reinnervation is more rapid in the portion of the denervated zone nearest the granule cells, where the maximal densities are attained within 30 days. The time course of reinnervation differed from that of degeneration. A portion of the new synapses in the reinnervated molecular layer appear to arise by the assembly of new synaptic junctions. Over time, the number of post-synaptic contact sites along a given length of dendritic surface recovers, suggesting the formation of new synaptic sites. Our data indicate that granule cells retain a capacity even into adulthood to manufacture, position and assemble postsynaptic components of a synapse and, in concert with reactive afferents, form normal-appearing synapses.", "contents": "An electron microscopic study of lesion-induced synaptogenesis in the dentate gyrus of the adult rat. II. Reappearance of morphologically normal synaptic contacts. Intact synapses in the denervated area of the rat dentate gyrus are reduced to 14% of those normally present 2-4 days following a unilateral entorhinal lesion. By 160-240 days after lesion, the former entorhinal terminal zone is repopulated with new synapses. In all, there is more than a 5-fold increase in the density of intact synapses in the denervated zone between 2 and 240 days post-lesion, and the denervated zone of the molecular layer is restored to 80% of control values. The synapses are Gray type I and are formed on simple and complex spines which closely resemble those normally present. A few boutons have an abnormally large number of synaptic junctions. Reinnervation seems to progress at differential rates. Synapses are rapidly regained up to 30 days after operation, but thereafter the reacquisition of synaptic connections is much slower. Reinnervation is more rapid in the portion of the denervated zone nearest the granule cells, where the maximal densities are attained within 30 days. The time course of reinnervation differed from that of degeneration. A portion of the new synapses in the reinnervated molecular layer appear to arise by the assembly of new synaptic junctions. Over time, the number of post-synaptic contact sites along a given length of dendritic surface recovers, suggesting the formation of new synaptic sites. Our data indicate that granule cells retain a capacity even into adulthood to manufacture, position and assemble postsynaptic components of a synapse and, in concert with reactive afferents, form normal-appearing synapses.", "PMID": 974742}
{"id": "pubmed23n0032_9952", "title": "Fine structure of the olfactory bulb in the goldfish, Carassius auratus.", "content": "Ultrastructural studies have aided in identifying the principal cell types and the layers of the olfactory bulb in goldfish. The concentrically arranged four layers are: the primary olfactory nerve layer (most peripheral), the glomerular layer, the mitral cell layer, and the granule cell layer (deepest). Three types of cell bodies were differentiated by their size and cytoplasmic organelles: mitral cells, granule cells and short axon cells. The axons of the primary olfactory nerve formed synaptic contacts with mitral cell dendrites in the glomeruli. Reciprocal synapses were observed between the granule cell dendrites and the mitral cell dendrites in the mitral cell layer. Terminals of the centrifugal fibers forming synaptic contacts with the granule cell dendrites were identified in the granule cell layer. The relationships between the cellular and synaptic organizations and the neural mechanism of olfaction are discussed.", "contents": "Fine structure of the olfactory bulb in the goldfish, Carassius auratus. Ultrastructural studies have aided in identifying the principal cell types and the layers of the olfactory bulb in goldfish. The concentrically arranged four layers are: the primary olfactory nerve layer (most peripheral), the glomerular layer, the mitral cell layer, and the granule cell layer (deepest). Three types of cell bodies were differentiated by their size and cytoplasmic organelles: mitral cells, granule cells and short axon cells. The axons of the primary olfactory nerve formed synaptic contacts with mitral cell dendrites in the glomeruli. Reciprocal synapses were observed between the granule cell dendrites and the mitral cell dendrites in the mitral cell layer. Terminals of the centrifugal fibers forming synaptic contacts with the granule cell dendrites were identified in the granule cell layer. The relationships between the cellular and synaptic organizations and the neural mechanism of olfaction are discussed.", "PMID": 974743}
{"id": "pubmed23n0032_9953", "title": "Tuning of single fibers in the cochlear nerve of the alligator lizard: relation to receptor morphology.", "content": "(1) The general anatomy of the peripheral portion of the cochlear nerve in the alligator lizard is described. (2) Spike discharges of single units were recorded with micropipets placed in the peripheral portion of the cochlear nerve of anesthetized lizards. (3) In response to tone bursts, each unit is maximally sensitive to a charactertistic frequency (CF). There are two distinct populations of units having different CFs: a low CF population (CF in the range 0.2-0.8 kHz) recorded in the portion of the nerve that enters the apical region of the basilar papilla and a high CF population (CF in the range 0.9-4.0 kHz) recorded in the portion of the nerve that enters the basal region. The low CF units are more sharply tuned than the high CF units. (4) Comparison of cochlear nerve units of the alligator lizard with those of mammals shows that the tuning of low CF units resembles that of mammalian units of the same CF. The tuning of high CF lizard units differs significantly from mammalian units. (5) The distinct differences in tuning of low and high CF units are correlated with distinct differences in the structure of the basilar papilla in the apical and basal regions rather than with differences in the width of the basilar membrane.", "contents": "Tuning of single fibers in the cochlear nerve of the alligator lizard: relation to receptor morphology. (1) The general anatomy of the peripheral portion of the cochlear nerve in the alligator lizard is described. (2) Spike discharges of single units were recorded with micropipets placed in the peripheral portion of the cochlear nerve of anesthetized lizards. (3) In response to tone bursts, each unit is maximally sensitive to a charactertistic frequency (CF). There are two distinct populations of units having different CFs: a low CF population (CF in the range 0.2-0.8 kHz) recorded in the portion of the nerve that enters the apical region of the basilar papilla and a high CF population (CF in the range 0.9-4.0 kHz) recorded in the portion of the nerve that enters the basal region. The low CF units are more sharply tuned than the high CF units. (4) Comparison of cochlear nerve units of the alligator lizard with those of mammals shows that the tuning of low CF units resembles that of mammalian units of the same CF. The tuning of high CF lizard units differs significantly from mammalian units. (5) The distinct differences in tuning of low and high CF units are correlated with distinct differences in the structure of the basilar papilla in the apical and basal regions rather than with differences in the width of the basilar membrane.", "PMID": 974744}
{"id": "pubmed23n0032_9954", "title": "Stimulation of the dorsal noradrenergic dundle and field potentials in the locus coeruleus.", "content": "Field potentials were recorded from the locus coeruleus following electrical stimulation of the rat midbrain. Activation of a discrete area situated ventrolateral to the central gray evoked long-latency potentials in the locus coeruleus while stimulation of other midbrain sites did not. Damage to this discrete area resulted in a depletion of norepinephrine in the cerebral cortex suggesting that it is a segment of the dorsal noradrenergic bundle arising from the locus coeruleus.", "contents": "Stimulation of the dorsal noradrenergic dundle and field potentials in the locus coeruleus. Field potentials were recorded from the locus coeruleus following electrical stimulation of the rat midbrain. Activation of a discrete area situated ventrolateral to the central gray evoked long-latency potentials in the locus coeruleus while stimulation of other midbrain sites did not. Damage to this discrete area resulted in a depletion of norepinephrine in the cerebral cortex suggesting that it is a segment of the dorsal noradrenergic bundle arising from the locus coeruleus.", "PMID": 974745}
{"id": "pubmed23n0032_9955", "title": "Preparation of cell bodies from the developing cerebellum: structural and metabolic integrity of the isolated cells.", "content": "A method is described for the isolation from the developing rat cerebellum of cell bodies which display a high level of ultrastructural organization. The procedure, which utilizes isotonic conditions throughout, begins with a brief trypsinisation at low enzyme concentration (0.025%). Proteolysis is terminated by trypsin inhibitor and followed by short exposure to EDTA. The technique is effective with cerebella from rats up to 2 weeks after birth. Recoveries of cell bodies vary from 130-410 million/g wet weight of tissue, depending on age: this represents, in terms of recovered DNA, a mean value for yield of 33%. Suspensions contain little debris, free nuclei are rare and about 80% of the perikarya excludes trypan blue. Survey electron micrographs show that most cell bodies possess uninterrupted plasma membrane profiles and retain highly organised cytoplasmic and nuclear ultrastructure. Structural preservation is highlighted in the case of Purkinje cell bodies in which may characteristic features survive including, most notably, perisomatic spines. Metabolic integrity appears to parallel morphological preservation as judged by several functional criteria, including the ability to metabolise glucose, accumulate K+ ions and synthesize proteins. SDS-polyacrylamide gel electrophoresis shows that the tissue dossociation technique does not lead to major deletions of cell proteins and that the pattern of perikaryal protein synthesis in vitro closely resembles that in vivo. These perikaryal preparations therefore hold out great promise as a simplified system for metabolic studies and as a starting material for the derivation of purified sub-populations of cell bodies from developing cerebellum.", "contents": "Preparation of cell bodies from the developing cerebellum: structural and metabolic integrity of the isolated cells. A method is described for the isolation from the developing rat cerebellum of cell bodies which display a high level of ultrastructural organization. The procedure, which utilizes isotonic conditions throughout, begins with a brief trypsinisation at low enzyme concentration (0.025%). Proteolysis is terminated by trypsin inhibitor and followed by short exposure to EDTA. The technique is effective with cerebella from rats up to 2 weeks after birth. Recoveries of cell bodies vary from 130-410 million/g wet weight of tissue, depending on age: this represents, in terms of recovered DNA, a mean value for yield of 33%. Suspensions contain little debris, free nuclei are rare and about 80% of the perikarya excludes trypan blue. Survey electron micrographs show that most cell bodies possess uninterrupted plasma membrane profiles and retain highly organised cytoplasmic and nuclear ultrastructure. Structural preservation is highlighted in the case of Purkinje cell bodies in which may characteristic features survive including, most notably, perisomatic spines. Metabolic integrity appears to parallel morphological preservation as judged by several functional criteria, including the ability to metabolise glucose, accumulate K+ ions and synthesize proteins. SDS-polyacrylamide gel electrophoresis shows that the tissue dossociation technique does not lead to major deletions of cell proteins and that the pattern of perikaryal protein synthesis in vitro closely resembles that in vivo. These perikaryal preparations therefore hold out great promise as a simplified system for metabolic studies and as a starting material for the derivation of purified sub-populations of cell bodies from developing cerebellum.", "PMID": 974746}
{"id": "pubmed23n0032_9956", "title": "Silver impregnation of dopaminergic systems after radiofrequency and 6-OHDA lesions of the rat ventral.", "content": "Nauta and Fink-Heimer silver impregnation techniques were used to study the anterior degeneration produced by radiofrequency (RF) or 6-OHDA lesions in the medial and lateral ventral mesencephalic tegmentum (VMT), substantia nigra and dorsalis tegmental decussation (DTD) in rats. Both Nauta and Fine-Heimer impregnating methods showed that RF lesion of the VNT produced degeneration in three major pathways: a ventral pathway corresponding to the fasciculus medialis prosencephali (FMP), an intermediate pathway projecting to the ventral thalamus, and a dorsal pathway to the medio-dorsal thalamus and to the nucleus lateralis habenulae. In addition, the Fink-Heimer method demonstrated prejections of the dopaminergic A10 and A9 cell group in the VMT to the nucleus caudatus after RF or 6-OHDA lesions. Projections to nucleus accumbens, tuberculum olfactorium, stria terminalis, and cortex frontalis were observed only after 6-OHDA lesion of the A10 cell group. Degeneration in cortex cinguli and entorhinalis was seen mainly after 6-OHDA lesion of the A9 cell group. The limbic forebrain cortical projections of the A10 group provide a coherent anatomical basis for the behavioral syndrome provoked by RF and 6-OHDA lesions in the VMT.", "contents": "Silver impregnation of dopaminergic systems after radiofrequency and 6-OHDA lesions of the rat ventral. Nauta and Fink-Heimer silver impregnation techniques were used to study the anterior degeneration produced by radiofrequency (RF) or 6-OHDA lesions in the medial and lateral ventral mesencephalic tegmentum (VMT), substantia nigra and dorsalis tegmental decussation (DTD) in rats. Both Nauta and Fine-Heimer impregnating methods showed that RF lesion of the VNT produced degeneration in three major pathways: a ventral pathway corresponding to the fasciculus medialis prosencephali (FMP), an intermediate pathway projecting to the ventral thalamus, and a dorsal pathway to the medio-dorsal thalamus and to the nucleus lateralis habenulae. In addition, the Fink-Heimer method demonstrated prejections of the dopaminergic A10 and A9 cell group in the VMT to the nucleus caudatus after RF or 6-OHDA lesions. Projections to nucleus accumbens, tuberculum olfactorium, stria terminalis, and cortex frontalis were observed only after 6-OHDA lesion of the A10 cell group. Degeneration in cortex cinguli and entorhinalis was seen mainly after 6-OHDA lesion of the A9 cell group. The limbic forebrain cortical projections of the A10 group provide a coherent anatomical basis for the behavioral syndrome provoked by RF and 6-OHDA lesions in the VMT.", "PMID": 974747}
{"id": "pubmed23n0032_9957", "title": "Nucleoside triphosphate and cation requirement for dopamine uptake by plain synaptic vesicles isolated from rat cerebrums.", "content": "Plain synaptic vesicles were partially purified from rat cerebrums and their [3H]dopamine uptake was investigated. The addition of MgCl2 plus ATP and of CaCl2 plus ATP increased the uptake 16 times and 5.5 times, respectively, higher than the control level, whereas the separate addition of these agents augmented the level at most 2.5 times. GTP stimulated the uptake as well as ATP, whereas UTP was one-third as effective and CTP, ADP, and AMP were all ineffective. Adenylyl imidodiphosphate was not only ineffective, but strongly reduced the ATP-dependent uptake. The half-maximal level of the ATP-dependent uptake was reached within 1.5 min after the start of incubation at 25 degrees C. The incorporation peaked at 5-10 min, then gradually declined to half-maximum at 45 min. This decline was prevented by the further addition of small amounts of CaCl2. There was no uptake at  0 degrees C, and the incorporation rate was at least 2 times faster at 37 degrees C than it was at 25 degrees C. The apparent Michaelis constant for [3H] dopamine was 1.6 muM. The half maximal inhibition of uptake was obtained at 0.1 muM reserpine; neither colchicine nor ouabain showed significant inhibition. The ATP-dependent uptake was not affected by K+, Na+, and Cl-, but was drastically decreased by isotonic phosphate buffer.", "contents": "Nucleoside triphosphate and cation requirement for dopamine uptake by plain synaptic vesicles isolated from rat cerebrums. Plain synaptic vesicles were partially purified from rat cerebrums and their [3H]dopamine uptake was investigated. The addition of MgCl2 plus ATP and of CaCl2 plus ATP increased the uptake 16 times and 5.5 times, respectively, higher than the control level, whereas the separate addition of these agents augmented the level at most 2.5 times. GTP stimulated the uptake as well as ATP, whereas UTP was one-third as effective and CTP, ADP, and AMP were all ineffective. Adenylyl imidodiphosphate was not only ineffective, but strongly reduced the ATP-dependent uptake. The half-maximal level of the ATP-dependent uptake was reached within 1.5 min after the start of incubation at 25 degrees C. The incorporation peaked at 5-10 min, then gradually declined to half-maximum at 45 min. This decline was prevented by the further addition of small amounts of CaCl2. There was no uptake at  0 degrees C, and the incorporation rate was at least 2 times faster at 37 degrees C than it was at 25 degrees C. The apparent Michaelis constant for [3H] dopamine was 1.6 muM. The half maximal inhibition of uptake was obtained at 0.1 muM reserpine; neither colchicine nor ouabain showed significant inhibition. The ATP-dependent uptake was not affected by K+, Na+, and Cl-, but was drastically decreased by isotonic phosphate buffer.", "PMID": 974748}
{"id": "pubmed23n0032_9958", "title": "Monosynaptic habituation in the vertebrate forebrain: the dentate gyrus examined in vitro.", "content": "The dentate gyrus of the hippocampus was examined for the presence of the parametric features of habituation. All 8 of the tested parameters were found in the dentate thus extending the observation of habituation to a monosynaptic junction in the vertebrate forebrain. The data are discussed in relation to hippocampal function and plasticity.", "contents": "Monosynaptic habituation in the vertebrate forebrain: the dentate gyrus examined in vitro. The dentate gyrus of the hippocampus was examined for the presence of the parametric features of habituation. All 8 of the tested parameters were found in the dentate thus extending the observation of habituation to a monosynaptic junction in the vertebrate forebrain. The data are discussed in relation to hippocampal function and plasticity.", "PMID": 974754}
{"id": "pubmed23n0032_9959", "title": "Lithium induced alterations in rat ganglionic lipids.", "content": "Superior cervical ganglia from normal or lithium fed rats were incubated in vitro with [U-14C]pyruvic acid, in Krebs-Ringer solution with or without lithium ion. Acute lithium incubations were performed on ganglia from normal rats with 25 mEquiv./l of lithium ion, while chronic incubations were performed on ganglia from lithium fed rats with 0.5 mEquiv./l lithium in the bathing solution. Lipids were extracted, separated by thin layer chromatography and specific activities of individual lipids and their fatty acids determined. In control ganglia shinogolipids contained 60-70% of the lipid radioactivity, glycerophospholipids 20-30% and neutral lipids 10-25%. Phosphatidylcholine contained 65% of the glycerophospholipid label and 40% of the lipid phosphorus, while sphingomyelin contained 90% of the sphingolipid label and exhibited the highest specific activity over all. Cholesterol was the major neutral lipid. Niety-five per cent of the glycerophospholipid label and 4% of the sphingomyelin label was localized in the fatty acids. The lipid and fatty acid compositions of all ganglia were similar. However the lipid radioactivity in chronic ganglia was lower than in control, with the sphingolipids most affected. In 80 min stimulated chronic ganglia stearic and oleic acid, radioactivity was depressed with respect to both control fatty acids and to the [14C]palmitate of chronic tissues. In both chronic and acute lithium ganglia stimulated for 80 min, the specific activities of phosphatidylinositol were significantly lower than in control. In contrast to control, the labeling of sphingolipids in resting acute ganglia was higher than in stimulated tissues.", "contents": "Lithium induced alterations in rat ganglionic lipids. Superior cervical ganglia from normal or lithium fed rats were incubated in vitro with [U-14C]pyruvic acid, in Krebs-Ringer solution with or without lithium ion. Acute lithium incubations were performed on ganglia from normal rats with 25 mEquiv./l of lithium ion, while chronic incubations were performed on ganglia from lithium fed rats with 0.5 mEquiv./l lithium in the bathing solution. Lipids were extracted, separated by thin layer chromatography and specific activities of individual lipids and their fatty acids determined. In control ganglia shinogolipids contained 60-70% of the lipid radioactivity, glycerophospholipids 20-30% and neutral lipids 10-25%. Phosphatidylcholine contained 65% of the glycerophospholipid label and 40% of the lipid phosphorus, while sphingomyelin contained 90% of the sphingolipid label and exhibited the highest specific activity over all. Cholesterol was the major neutral lipid. Niety-five per cent of the glycerophospholipid label and 4% of the sphingomyelin label was localized in the fatty acids. The lipid and fatty acid compositions of all ganglia were similar. However the lipid radioactivity in chronic ganglia was lower than in control, with the sphingolipids most affected. In 80 min stimulated chronic ganglia stearic and oleic acid, radioactivity was depressed with respect to both control fatty acids and to the [14C]palmitate of chronic tissues. In both chronic and acute lithium ganglia stimulated for 80 min, the specific activities of phosphatidylinositol were significantly lower than in control. In contrast to control, the labeling of sphingolipids in resting acute ganglia was higher than in stimulated tissues.", "PMID": 974755}
{"id": "pubmed23n0032_9960", "title": "Developmental studies of the compartmentalization of water and electrolytes in the choroid plexus of the neonatal rat brain.", "content": "Rats of various postnatal ages were utilized to study developmental changes in the distribution of Na, K and H2O between the various compartments of the lateral ventricular plexus (LVP). During the 3 weeks after birth, as the LVP grows from 0.5 to 0.8 mg, there is a significant increase in plexus K which is accompanied by a progressive decrease in Na and H2O. Also, during this postnatal period the decrease in [3h]inulin space in the plexus is proportional to the decrease in the Na space. Between 3 weeks and adulthood, the [3h]inulin and Na spaces are both augmented to a similar extent; moreover, during this same period of development there is a trebling of the residual [51cr]erythrocyte volume. Despite the substantial changes in the volume of the extracellular fluid and of the residual blood in the plexus with age, the calculated concentrations (mEquiv./kg H2O) of choroid cell Na (30-35) and K (145-155) are similar for all ages investigated. The derived data for cellular ionic concentration, together with the analysis of the ionic concentration gradients (cerebrospinal fluid/plasma H2O), suggest that the transport mechanism which translocates Na and K across the choroidal membrane is operative as early as 3-4 days postnatal. The important role of the choroid plexus in central nervous system homeostasis is discussed in relation to the developing brain.", "contents": "Developmental studies of the compartmentalization of water and electrolytes in the choroid plexus of the neonatal rat brain. Rats of various postnatal ages were utilized to study developmental changes in the distribution of Na, K and H2O between the various compartments of the lateral ventricular plexus (LVP). During the 3 weeks after birth, as the LVP grows from 0.5 to 0.8 mg, there is a significant increase in plexus K which is accompanied by a progressive decrease in Na and H2O. Also, during this postnatal period the decrease in [3h]inulin space in the plexus is proportional to the decrease in the Na space. Between 3 weeks and adulthood, the [3h]inulin and Na spaces are both augmented to a similar extent; moreover, during this same period of development there is a trebling of the residual [51cr]erythrocyte volume. Despite the substantial changes in the volume of the extracellular fluid and of the residual blood in the plexus with age, the calculated concentrations (mEquiv./kg H2O) of choroid cell Na (30-35) and K (145-155) are similar for all ages investigated. The derived data for cellular ionic concentration, together with the analysis of the ionic concentration gradients (cerebrospinal fluid/plasma H2O), suggest that the transport mechanism which translocates Na and K across the choroidal membrane is operative as early as 3-4 days postnatal. The important role of the choroid plexus in central nervous system homeostasis is discussed in relation to the developing brain.", "PMID": 974769}
{"id": "pubmed23n0032_9961", "title": "Effect of auditory neocortex ablation on identification of click rates in cats.", "content": "Six experimental and 3 unoperated cats were trained with a go, no-go shock avoidance procedure to discriminate increases from decreases in the rate of presentation of all auditory cortex between the suprasylvian sulcus and rhinal fissure while 3 cats had bilateral auditory cortex lesions plus ablation of the cortex of the anterior lateral and anterior and middle suprasylvian gyri. A sixth 'naive' experimental cat received the present tests only after recovery from a bilateral auditory cortex ablation. After bilateral lesions, 5 of the experimental cats unexpectedly made no errors on no-go trials during retraining. This contrasts with their preoperative performance, as well as the performance of the 3 unoperated and the 'naive' operated cat, in which training was required for the successful discrimination of the two types of trials. This suggests that the neocortex may be more critical for mediating active 'go' responses to auditory stimuli than in preserving a memory for the difference between go and no-go stimuli. Further testing revealed that the thresholds of the operated cats did not differ from normal cats. All cats discriminated rates of 4/sec versus 6/sec clicks both with and without a neutral 5/sec background at levels significantly above chance.", "contents": "Effect of auditory neocortex ablation on identification of click rates in cats. Six experimental and 3 unoperated cats were trained with a go, no-go shock avoidance procedure to discriminate increases from decreases in the rate of presentation of all auditory cortex between the suprasylvian sulcus and rhinal fissure while 3 cats had bilateral auditory cortex lesions plus ablation of the cortex of the anterior lateral and anterior and middle suprasylvian gyri. A sixth 'naive' experimental cat received the present tests only after recovery from a bilateral auditory cortex ablation. After bilateral lesions, 5 of the experimental cats unexpectedly made no errors on no-go trials during retraining. This contrasts with their preoperative performance, as well as the performance of the 3 unoperated and the 'naive' operated cat, in which training was required for the successful discrimination of the two types of trials. This suggests that the neocortex may be more critical for mediating active 'go' responses to auditory stimuli than in preserving a memory for the difference between go and no-go stimuli. Further testing revealed that the thresholds of the operated cats did not differ from normal cats. All cats discriminated rates of 4/sec versus 6/sec clicks both with and without a neutral 5/sec background at levels significantly above chance.", "PMID": 974770}
{"id": "pubmed23n0032_9962", "title": "Chronic changes in the response of cells in adult cat dorsal horn following partial deafferentation: the appearance of responding cells in a previously non-responsive region.", "content": "One side of the lumbar enlargement in adult cats was partially deafferented by cutting all dorsal roots caudal to L3 with the exception of the S1 dorsal root. At various times after the roots had been sectioned, the response of dorsal horn  cells to natural and electrical stimuli applied to the leg and flank were recorded through extracellular glass microelectrodes. When animals were examined up to 24 h after this partial deafferentation, no cells were located in a region between segments L4 and 5 which responded monosynaptically to cutaneous stimulation on the leg. By one week, cells began to appear in the L4-5 region which responded monosynpatically to peripheral stimuli. The numbers of these newly connected cells seemed to have stabilised by 1 month after the partial deafferentation, but the properties of these cells were abnormal in 6 ways. The location of the receptive field of the cells was characteristic either of the S1 dermatome or of segments rostral to L4. Some cells had double receptive fields, one on the leg and one of the abdomen. The size of the receptive field varied more than is observed in normal intact dorsal horn. In particular, certain cells had unusually small recpetive fields with abrupt edges and no associated inhibitory fields. The cells receive less convergence from high treshold afferents than normally observed. Associated inhibitory fields were rarely encountered. Habituation was observed and in some cells with doulbe receptive fields the response o onet area habituated while the response to the other area was unaffected. Slow wave recording on the surface of the cord showed that the effect of peripheral stimulation of the S1 dermatome spread far more extensively on the chronically deafferented side of the cord than it did on the intact side or in an intact cord. It was concluded that following partial deafferentation, the remaining afferents can establish connection with deafferented cells but the data presented did not allow a conclusion as to whether the new connections were produced by sprouting or by the unmasking of existing connections.", "contents": "Chronic changes in the response of cells in adult cat dorsal horn following partial deafferentation: the appearance of responding cells in a previously non-responsive region. One side of the lumbar enlargement in adult cats was partially deafferented by cutting all dorsal roots caudal to L3 with the exception of the S1 dorsal root. At various times after the roots had been sectioned, the response of dorsal horn  cells to natural and electrical stimuli applied to the leg and flank were recorded through extracellular glass microelectrodes. When animals were examined up to 24 h after this partial deafferentation, no cells were located in a region between segments L4 and 5 which responded monosynaptically to cutaneous stimulation on the leg. By one week, cells began to appear in the L4-5 region which responded monosynpatically to peripheral stimuli. The numbers of these newly connected cells seemed to have stabilised by 1 month after the partial deafferentation, but the properties of these cells were abnormal in 6 ways. The location of the receptive field of the cells was characteristic either of the S1 dermatome or of segments rostral to L4. Some cells had double receptive fields, one on the leg and one of the abdomen. The size of the receptive field varied more than is observed in normal intact dorsal horn. In particular, certain cells had unusually small recpetive fields with abrupt edges and no associated inhibitory fields. The cells receive less convergence from high treshold afferents than normally observed. Associated inhibitory fields were rarely encountered. Habituation was observed and in some cells with doulbe receptive fields the response o onet area habituated while the response to the other area was unaffected. Slow wave recording on the surface of the cord showed that the effect of peripheral stimulation of the S1 dermatome spread far more extensively on the chronically deafferented side of the cord than it did on the intact side or in an intact cord. It was concluded that following partial deafferentation, the remaining afferents can establish connection with deafferented cells but the data presented did not allow a conclusion as to whether the new connections were produced by sprouting or by the unmasking of existing connections.", "PMID": 974771}
{"id": "pubmed23n0032_9963", "title": "Responses of complex cells in the visual cortex of the cat as a function of the length of moving slits.", "content": "(1) As a step towards specifying the spatial selectivity characteristics of complex cells with spatially periodic substructures, we have studied single cell responses to narrow slits of variable length moved across the receptive field in the preferred direction. In general, the length-response curves were linear over a considerable and sometimes full range until an optimal slit length was reached. (2) In those cells in which the rate of rise of the slit length-response functions decreased before the optimal length was reached, at least 3 factors contribute to the shape of the curve. First, the receptive field shapes of some complex cells are more ovoid or rounded than rectangular, and the summation of responses from excitatory zones of varying optimal lengths itself results in a nonlinear slit length-response function at long slit lengths. Second, central regions may contribute more to cell response than do more lateral regions along the length dimension. Third, a nonlinearity in the slit length-response curve may occur in the upper range of slit lengths as a saturation effect because discharge rates may reach 600/sec, which appears to be close to a limiting firing rate. (3) Some cells believed to be complex during preliminary receptive field testing showed weak inhibitory regions beyond the region of the optimal slit length. Many of these cells also displayed periodic average response histograms to moving slits. The extent and magnitude of the inhibition were variable from cell to cell. In terms of receptive field properties, these cells and 'regular' complex cells seem part of a continuum.", "contents": "Responses of complex cells in the visual cortex of the cat as a function of the length of moving slits. (1) As a step towards specifying the spatial selectivity characteristics of complex cells with spatially periodic substructures, we have studied single cell responses to narrow slits of variable length moved across the receptive field in the preferred direction. In general, the length-response curves were linear over a considerable and sometimes full range until an optimal slit length was reached. (2) In those cells in which the rate of rise of the slit length-response functions decreased before the optimal length was reached, at least 3 factors contribute to the shape of the curve. First, the receptive field shapes of some complex cells are more ovoid or rounded than rectangular, and the summation of responses from excitatory zones of varying optimal lengths itself results in a nonlinear slit length-response function at long slit lengths. Second, central regions may contribute more to cell response than do more lateral regions along the length dimension. Third, a nonlinearity in the slit length-response curve may occur in the upper range of slit lengths as a saturation effect because discharge rates may reach 600/sec, which appears to be close to a limiting firing rate. (3) Some cells believed to be complex during preliminary receptive field testing showed weak inhibitory regions beyond the region of the optimal slit length. Many of these cells also displayed periodic average response histograms to moving slits. The extent and magnitude of the inhibition were variable from cell to cell. In terms of receptive field properties, these cells and 'regular' complex cells seem part of a continuum.", "PMID": 974772}
{"id": "pubmed23n0032_9964", "title": "The effects of pentylenetetrazol on molluscan neurons. I. Intracellular recording and stimulation.", "content": "The effects of the convulsant drug pentylenetetrazol (PTZ) were studied in neurons from isolated ganglia of the nudibranch molluscs, Archidoris montereyensis and Anisodoris nobilis, using conventional techniques of intracellular recording and constant current stimulation. PTZ was selected because it causes changes in the intracellularly recorded responses similar to the depolarization shifts recorded in mammalian epileptic neurons. When perfusate containing 120-140 mM PTZ is introduced, the intracellular recording is characterized by an initial silent period followed by small oscillations in membrane potential and irregular firing of spikes. Within 5-15 min, bursts of 2-3 spikes occurred followed by the appearance of episodic prolonged depolarizations with superimposed high-frequency spikes. In the presence of PTZ the prolonged depolarizations were evoked by intracellular stimulation and at the termination of conditioning hyperpolarizations. The prolonged depolarizations were also recorded in neurons isolated from all synpatic input by axonal ligation. Prolonged depolarizations showed threshold behavior since they can be terminated early by an intracellularly applied hyperpolarizing current.", "contents": "The effects of pentylenetetrazol on molluscan neurons. I. Intracellular recording and stimulation. The effects of the convulsant drug pentylenetetrazol (PTZ) were studied in neurons from isolated ganglia of the nudibranch molluscs, Archidoris montereyensis and Anisodoris nobilis, using conventional techniques of intracellular recording and constant current stimulation. PTZ was selected because it causes changes in the intracellularly recorded responses similar to the depolarization shifts recorded in mammalian epileptic neurons. When perfusate containing 120-140 mM PTZ is introduced, the intracellular recording is characterized by an initial silent period followed by small oscillations in membrane potential and irregular firing of spikes. Within 5-15 min, bursts of 2-3 spikes occurred followed by the appearance of episodic prolonged depolarizations with superimposed high-frequency spikes. In the presence of PTZ the prolonged depolarizations were evoked by intracellular stimulation and at the termination of conditioning hyperpolarizations. The prolonged depolarizations were also recorded in neurons isolated from all synpatic input by axonal ligation. Prolonged depolarizations showed threshold behavior since they can be terminated early by an intracellularly applied hyperpolarizing current.", "PMID": 974773}
{"id": "pubmed23n0032_9965", "title": "The effects of pentylenetetrazol on molluscan neurons. II. Voltage clamp studies.", "content": "The effects of pentylenetetrazol (PTZ) upon the steady and transient outward ionic currents during PTZ-induced prolonged depolarizations were investigated using voltage clamp techniques. PTZ causes a 5-35% reduction in gL and a 40-60% reduction in steady-state gK. There is also a marked reduction in the activation of gA of Connor and Stevens6 at all clamp potentials; a shortening of the time constant for the inactivation of gA; and a 10-15 mV shift in the depolarizing direction of the curve relating the steady-state inactivation of gA to membrane potential. The equilibrium potentials for both gA and gK are depolarized by 20 mV in PTZ solution. Equation and voltage clamp data for normal repetitive firing were integrated with the normal and PTZ-alered data. Solution to these equations demonstrated: (1) normal repetitive firing in response to a constant current stimulus; and (2) PTZ-altered repetitive firing that was in the direction of, and for the most part, similar to the observed behavior.", "contents": "The effects of pentylenetetrazol on molluscan neurons. II. Voltage clamp studies. The effects of pentylenetetrazol (PTZ) upon the steady and transient outward ionic currents during PTZ-induced prolonged depolarizations were investigated using voltage clamp techniques. PTZ causes a 5-35% reduction in gL and a 40-60% reduction in steady-state gK. There is also a marked reduction in the activation of gA of Connor and Stevens6 at all clamp potentials; a shortening of the time constant for the inactivation of gA; and a 10-15 mV shift in the depolarizing direction of the curve relating the steady-state inactivation of gA to membrane potential. The equilibrium potentials for both gA and gK are depolarized by 20 mV in PTZ solution. Equation and voltage clamp data for normal repetitive firing were integrated with the normal and PTZ-alered data. Solution to these equations demonstrated: (1) normal repetitive firing in response to a constant current stimulus; and (2) PTZ-altered repetitive firing that was in the direction of, and for the most part, similar to the observed behavior.", "PMID": 974774}
{"id": "pubmed23n0032_9966", "title": "Incorporation of tritiated leucine by axotomized rubral neurons.", "content": "Fourteen kittens, 7--10 weeks of age, were injected with [3H]leucine 0.5--24 h before sacrifice 1--30 days after unilateral high cervical rubrospinal tractotomy. Histoautoradiographs of the red nuclei were prepared and counterstained with thionin. Axon reaction, evident histologically 24 h after surgery, was manifested by central chromatolysis or diffuse cytoplasmic chromophobia. Partial reversion toward a normal cytologic appearance was apparent 10--30 days postoperatively. Nucleolar and nuclear shrinkage and cytoplasmic atrophy were conspicuous accompaniments of axon reaction in rubral neurons. Expressed per cell the radioactivity of axotomized rubral nerve cells was consistently less than controls in animals surviving operation from 5 to 30 days. The data indicate that axon reaction in red nucleus is regressive in character and early associated with diminished protein synthesis. The frequently regressive nature of axon reaction in intrinsic neurons, such as those of red nucleus, probably is important in accounting for failure of regeneration of many mammalian CNS fiber tracts after injury.", "contents": "Incorporation of tritiated leucine by axotomized rubral neurons. Fourteen kittens, 7--10 weeks of age, were injected with [3H]leucine 0.5--24 h before sacrifice 1--30 days after unilateral high cervical rubrospinal tractotomy. Histoautoradiographs of the red nuclei were prepared and counterstained with thionin. Axon reaction, evident histologically 24 h after surgery, was manifested by central chromatolysis or diffuse cytoplasmic chromophobia. Partial reversion toward a normal cytologic appearance was apparent 10--30 days postoperatively. Nucleolar and nuclear shrinkage and cytoplasmic atrophy were conspicuous accompaniments of axon reaction in rubral neurons. Expressed per cell the radioactivity of axotomized rubral nerve cells was consistently less than controls in animals surviving operation from 5 to 30 days. The data indicate that axon reaction in red nucleus is regressive in character and early associated with diminished protein synthesis. The frequently regressive nature of axon reaction in intrinsic neurons, such as those of red nucleus, probably is important in accounting for failure of regeneration of many mammalian CNS fiber tracts after injury.", "PMID": 974775}
{"id": "pubmed23n0032_9967", "title": "Regional distribution of substance P in the brain of the rat.", "content": "Using a sensitive radioimmunoassay we have studied the regional distribution of substance P. The level of substance P is higher in the mesencephalon, hypothalamus and preoptic area than in other regions of the brain. Substance P is found in especially high concentrations in the reticular part of the substantia nigra and the interpeduncular nucleus. It is present in large amounts in several septal, preoptic and hypothalamic nuclei as well.", "contents": "Regional distribution of substance P in the brain of the rat. Using a sensitive radioimmunoassay we have studied the regional distribution of substance P. The level of substance P is higher in the mesencephalon, hypothalamus and preoptic area than in other regions of the brain. Substance P is found in especially high concentrations in the reticular part of the substantia nigra and the interpeduncular nucleus. It is present in large amounts in several septal, preoptic and hypothalamic nuclei as well.", "PMID": 974776}
{"id": "pubmed23n0032_9968", "title": "The growth of the dendritic trees of Purkinje cells in irradiated agranular cerebellar cortex.", "content": "The heads of noenatal Wistar rats were irradiated with 200 rads daily from birth to the 10th day post-partum. Ten litters each containing 5 animals were killed at 30 days post-partum and their brains treated by the Golgi-Cox technique. The dendritic trees of 24 Purkinje cells were analysed using the quantitative technique of network analysis, and comparisons made between parameters obtained from 20 normal Purkinje cells. All dendritic trees in agranular irradiated cortex were markedly reduced in size (as indicated by total dendritic length and total number of segments) although mean path lengths were normal. Segment lengths were normal over proximal branches, but uniformly increased over distal branches. Abnormal appendages, called 'giant spines' were observed on many dendrites. They were often some 10 mum in length and their presence effectively reduced segment lengths, increased the frequency of trichotomy and deviated growth from the normal random terminal pattern so that long collateral branching topologies were formed. Nevertheless, trichotomy was uniformly reduced in those trees without 'giant spines' and the distribution of branching patterns suggested that growth had proceeded by random terminal dichotomy. These results demonstrate that the development of dendritic trees is retarded in the agranular irradiated cerebellum, where synaptogenesis is very greatly reduced below normal. The quantitative changes in segment lengths, size of trees, and trichotomy accord with those predicted by the filopodial synaptogenic hypothesis of dendritic growth formulated by Vaughn et al. 99, whilst the results of the topological analysis suggest that branching is established by a degree of non-random interaction between growing dendrites and their substrate. 'Claw-like' dendritic complexes within some Purkinje cell trees may have been induced by aberrent fibre bundles of few surviving granule cells.", "contents": "The growth of the dendritic trees of Purkinje cells in irradiated agranular cerebellar cortex. The heads of noenatal Wistar rats were irradiated with 200 rads daily from birth to the 10th day post-partum. Ten litters each containing 5 animals were killed at 30 days post-partum and their brains treated by the Golgi-Cox technique. The dendritic trees of 24 Purkinje cells were analysed using the quantitative technique of network analysis, and comparisons made between parameters obtained from 20 normal Purkinje cells. All dendritic trees in agranular irradiated cortex were markedly reduced in size (as indicated by total dendritic length and total number of segments) although mean path lengths were normal. Segment lengths were normal over proximal branches, but uniformly increased over distal branches. Abnormal appendages, called 'giant spines' were observed on many dendrites. They were often some 10 mum in length and their presence effectively reduced segment lengths, increased the frequency of trichotomy and deviated growth from the normal random terminal pattern so that long collateral branching topologies were formed. Nevertheless, trichotomy was uniformly reduced in those trees without 'giant spines' and the distribution of branching patterns suggested that growth had proceeded by random terminal dichotomy. These results demonstrate that the development of dendritic trees is retarded in the agranular irradiated cerebellum, where synaptogenesis is very greatly reduced below normal. The quantitative changes in segment lengths, size of trees, and trichotomy accord with those predicted by the filopodial synaptogenic hypothesis of dendritic growth formulated by Vaughn et al. 99, whilst the results of the topological analysis suggest that branching is established by a degree of non-random interaction between growing dendrites and their substrate. 'Claw-like' dendritic complexes within some Purkinje cell trees may have been induced by aberrent fibre bundles of few surviving granule cells.", "PMID": 974782}
{"id": "pubmed23n0032_9969", "title": "Regional differences in the morphology of the rat subfornical organ.", "content": "Based upon scanning and transmission electron microscopy 3 regions are distinguished in the rat subfornical organ. The rostral region is dominated by nerve fibers interspersed with relatively few neurons and glial cells. Squamous to low cuboidal ependymal cells with flat ventricular surfaces bearing a few short microvilli line the center of this region; laterally, ciliated cuboidal ependymal cells predominate. The central region occupies the largest area of the organ and contains most of the neuronal perikarya and glial cells. Many perikarya and neuronal processes are located immediately underneath the ependymal surface. A dense capillary network with wide pericapillary spaces permeates the tissue. In the rostral two-thirds of this region the ependymal cells are either squamous or cuboidal, sometimes with slightly bulging ventricular surfaces bearing longer microvilli. Here supraependymal neurons are particularly numerous. The caudal one-third of the central region is characterized by squamous, cuboidal and columnar ependymal cells whose hemispherical ventricular surfaces are studded with long microvilli and occasional cilia and vesicular protrusions. The caudal region, like the rostral region, is dominated by nerve fibers between which neuronal perikarya and glial cells are present. At this level the choroid plexus is attached to the SFO through highly vascularized pial connective tissue. It is the major point of penetration of the SFO's capillary plexus. The possible significance of these observations and their importance in experimental interventions are discussed.", "contents": "Regional differences in the morphology of the rat subfornical organ. Based upon scanning and transmission electron microscopy 3 regions are distinguished in the rat subfornical organ. The rostral region is dominated by nerve fibers interspersed with relatively few neurons and glial cells. Squamous to low cuboidal ependymal cells with flat ventricular surfaces bearing a few short microvilli line the center of this region; laterally, ciliated cuboidal ependymal cells predominate. The central region occupies the largest area of the organ and contains most of the neuronal perikarya and glial cells. Many perikarya and neuronal processes are located immediately underneath the ependymal surface. A dense capillary network with wide pericapillary spaces permeates the tissue. In the rostral two-thirds of this region the ependymal cells are either squamous or cuboidal, sometimes with slightly bulging ventricular surfaces bearing longer microvilli. Here supraependymal neurons are particularly numerous. The caudal one-third of the central region is characterized by squamous, cuboidal and columnar ependymal cells whose hemispherical ventricular surfaces are studded with long microvilli and occasional cilia and vesicular protrusions. The caudal region, like the rostral region, is dominated by nerve fibers between which neuronal perikarya and glial cells are present. At this level the choroid plexus is attached to the SFO through highly vascularized pial connective tissue. It is the major point of penetration of the SFO's capillary plexus. The possible significance of these observations and their importance in experimental interventions are discussed.", "PMID": 974783}
{"id": "pubmed23n0032_9970", "title": "Convergence of the descending pathways of motor, visual and limbic cortex in the cat di- and mesencephalon.", "content": "Comparisons of the descending pathways of the motor, visual and limbic cortex in the cat revealed a number of structures in di- and mesencephalon that receive projections from all these fields. They are centrolateral (CL), paracentral (Pc), central medial (CeM) and centre median (CM), nuclei of the thalamus, zona incerta (ZI) and Forel's field H1 of the subthalamus, nucleus of the posterior commissure and anterior and posterior nuclei of the pretectum (Prt), stratum griseum intermedial and profundum of superior colliculus (CS), periaqueductal gray matter (GC) and pontine nuclei (NP). The projections from the striate field of the visual cortex to ventrobasal complex of the thalamus (VB) are shown. The results are discussed with recent data on the anatomy and physiology. A possible role of corticocortical and corticofugal fiber connections is dealt with.", "contents": "Convergence of the descending pathways of motor, visual and limbic cortex in the cat di- and mesencephalon. Comparisons of the descending pathways of the motor, visual and limbic cortex in the cat revealed a number of structures in di- and mesencephalon that receive projections from all these fields. They are centrolateral (CL), paracentral (Pc), central medial (CeM) and centre median (CM), nuclei of the thalamus, zona incerta (ZI) and Forel's field H1 of the subthalamus, nucleus of the posterior commissure and anterior and posterior nuclei of the pretectum (Prt), stratum griseum intermedial and profundum of superior colliculus (CS), periaqueductal gray matter (GC) and pontine nuclei (NP). The projections from the striate field of the visual cortex to ventrobasal complex of the thalamus (VB) are shown. The results are discussed with recent data on the anatomy and physiology. A possible role of corticocortical and corticofugal fiber connections is dealt with.", "PMID": 974784}
{"id": "pubmed23n0032_9971", "title": "Response plasticity in hamster olfactory bulb: peripheral and central processes.", "content": "It is a well-established fact that prolonged odor stimulation leads to marked sensory adaptation. This study demonstrates comparable electrophysiological phenomena occurring at the level of the olfactory receptor and at more central olfactory structures. Recordings of overall receptor response and of olfactory bulb unit responses were made during repeated odor stimulation. During the course of a single, continuous odor presentation response decrements were seen in the EOG (at the olfactory receptors) and were mirrored at the mitral cell layer of the bulb. When brief periods without stimulation were introduced between such odor presentations, receptor responsiveness rebounded to its original level, but mitral cell responses did not. On the basis of this dissociation it is suggested that the pattern of response decrement within the bulb represents a case of stimulus-specific habituation in a simple cortical subsystem and is well worth future investigation as a model of neural plasticity. Surgical disconnection of the olfactory bulb from one or more of its centrifugal inputs results in hyperactive, hyperresponsive mitral cells, which habituate more rapidly and show longer recovery times than do those in the intact bulb. In addition, the synchronization of such units to the inhalation cycle is markedly reduced as compared with normal preparations. These facts together suggest that the habituation of mitral cell activity does not depend on centrifugal inputs, although one or more of such inputs act indirectly in an inhibitory fashion to modulate and tune mitral cell response characteristics.", "contents": "Response plasticity in hamster olfactory bulb: peripheral and central processes. It is a well-established fact that prolonged odor stimulation leads to marked sensory adaptation. This study demonstrates comparable electrophysiological phenomena occurring at the level of the olfactory receptor and at more central olfactory structures. Recordings of overall receptor response and of olfactory bulb unit responses were made during repeated odor stimulation. During the course of a single, continuous odor presentation response decrements were seen in the EOG (at the olfactory receptors) and were mirrored at the mitral cell layer of the bulb. When brief periods without stimulation were introduced between such odor presentations, receptor responsiveness rebounded to its original level, but mitral cell responses did not. On the basis of this dissociation it is suggested that the pattern of response decrement within the bulb represents a case of stimulus-specific habituation in a simple cortical subsystem and is well worth future investigation as a model of neural plasticity. Surgical disconnection of the olfactory bulb from one or more of its centrifugal inputs results in hyperactive, hyperresponsive mitral cells, which habituate more rapidly and show longer recovery times than do those in the intact bulb. In addition, the synchronization of such units to the inhalation cycle is markedly reduced as compared with normal preparations. These facts together suggest that the habituation of mitral cell activity does not depend on centrifugal inputs, although one or more of such inputs act indirectly in an inhibitory fashion to modulate and tune mitral cell response characteristics.", "PMID": 974785}
{"id": "pubmed23n0032_9972", "title": "The contribution of local blood flow to the rapid clearance of potassium from the cortical extracellular space.", "content": "The transport of potassium to the blood stream following stimulation of the cortex in cats is evaluated by means of a potassium sensitive microelectrode technique. Potassium levels are measured in cortical veins, the sagittal sinus and the extracellular space during and after both pharmacological and electrical stimulation of the cortex. It is concluded that the potassium transport to the blood stream is not a significant factor in the rapid clearance of potassium from the extracellular space following stimulation.", "contents": "The contribution of local blood flow to the rapid clearance of potassium from the cortical extracellular space. The transport of potassium to the blood stream following stimulation of the cortex in cats is evaluated by means of a potassium sensitive microelectrode technique. Potassium levels are measured in cortical veins, the sagittal sinus and the extracellular space during and after both pharmacological and electrical stimulation of the cortex. It is concluded that the potassium transport to the blood stream is not a significant factor in the rapid clearance of potassium from the extracellular space following stimulation.", "PMID": 974786}
{"id": "pubmed23n0032_9973", "title": "On spinal noradrenaline receptor supersensitivity: correlation between nerve terminal densities and flexor reflexes various times after intracisternal 6-hydroxydopamine.", "content": "The noradrenaline (NA)-dependent hindlimb flexor reflex that can be elicited by pinching the foot of acutely spinalized rats given nialamide-DOPA or clonidine was evaluated different time intervals (14 days-6 months) after intracisternal injections of 6-OH-dopamine (6-OH-DA) and correlated to the degree of bulbospinal catecholamine (CA) denervation as seen by Falck-Hillarp fluorescence histochemistry. Six and 14 days after 6-OH-DA, when almost all NA nerve terminals of the spinal cord had degenerated, the NA receptors where supersensitive to stimulation with clonidine as evidenced by an increased flexor reflex. This supersensitivity gradually disappeared as new nerve terminals were formed in the grey matter of the spinal cord during the following 3-6 months. The supersensitivity phenomenon 14 days after 6-OH-DA could also be demonstrated by L-DOPA given to animals pretreated with 100 mg/kg nialamide. Using this relatively low dose of nialamide, almost no reflex response was seen in the control group. Using a higher degree of monoaminoxidase inhibition (nialamide 200 mg/kg) also non-supersensitive, NA receptors became maximally stimulated. Therefore, 6-OH-DA treated rats now showed a weaker reflex than controls, the reflex response being directly correlated to the number of nerve terminals present that could form NA from the precursor. Using 5,6-dihydroxytryptamine, which selectively destroys 5-hydroxytryptamine (5-HT) nerves, it was shown that the flexor reflex changes were specifically related to the NA nerves and unchanged by the simultaneous presence or absence of 5-HT nerve terminals. This was further supported by the finding of a correlation between amount of nerve terminals and flexor reflex responses in individual animals, especially at longer survival times both in the clonidine and the nialamide-DOPA experiments.", "contents": "On spinal noradrenaline receptor supersensitivity: correlation between nerve terminal densities and flexor reflexes various times after intracisternal 6-hydroxydopamine. The noradrenaline (NA)-dependent hindlimb flexor reflex that can be elicited by pinching the foot of acutely spinalized rats given nialamide-DOPA or clonidine was evaluated different time intervals (14 days-6 months) after intracisternal injections of 6-OH-dopamine (6-OH-DA) and correlated to the degree of bulbospinal catecholamine (CA) denervation as seen by Falck-Hillarp fluorescence histochemistry. Six and 14 days after 6-OH-DA, when almost all NA nerve terminals of the spinal cord had degenerated, the NA receptors where supersensitive to stimulation with clonidine as evidenced by an increased flexor reflex. This supersensitivity gradually disappeared as new nerve terminals were formed in the grey matter of the spinal cord during the following 3-6 months. The supersensitivity phenomenon 14 days after 6-OH-DA could also be demonstrated by L-DOPA given to animals pretreated with 100 mg/kg nialamide. Using this relatively low dose of nialamide, almost no reflex response was seen in the control group. Using a higher degree of monoaminoxidase inhibition (nialamide 200 mg/kg) also non-supersensitive, NA receptors became maximally stimulated. Therefore, 6-OH-DA treated rats now showed a weaker reflex than controls, the reflex response being directly correlated to the number of nerve terminals present that could form NA from the precursor. Using 5,6-dihydroxytryptamine, which selectively destroys 5-hydroxytryptamine (5-HT) nerves, it was shown that the flexor reflex changes were specifically related to the NA nerves and unchanged by the simultaneous presence or absence of 5-HT nerve terminals. This was further supported by the finding of a correlation between amount of nerve terminals and flexor reflex responses in individual animals, especially at longer survival times both in the clonidine and the nialamide-DOPA experiments.", "PMID": 974787}
{"id": "pubmed23n0032_9974", "title": "Long lasting changes in the spontaneous activity of hippocampal neurons following stimulation of the entorhinal cortex.", "content": "The spontaneous activity of hippocampal and denate units was investigated in acute and chronic experiments on adult rats. Spontaneous unitary discharges from both regions were measured before and after brief tetanic stimulation of the entorhinal cortex. An increase in the frequency of spontaneous unit activity was obtained in units recorded from the dentate gyrus and CA3 field of the hippocampus for up to 20 min following stimulation. In chronic recordings of physiologically identified dentate units from freely moving rats similar results were obtained. The findings are discussed with reference to long lasting potentiation of synaptic activity in the perforant path-dentate granule cell connection.", "contents": "Long lasting changes in the spontaneous activity of hippocampal neurons following stimulation of the entorhinal cortex. The spontaneous activity of hippocampal and denate units was investigated in acute and chronic experiments on adult rats. Spontaneous unitary discharges from both regions were measured before and after brief tetanic stimulation of the entorhinal cortex. An increase in the frequency of spontaneous unit activity was obtained in units recorded from the dentate gyrus and CA3 field of the hippocampus for up to 20 min following stimulation. In chronic recordings of physiologically identified dentate units from freely moving rats similar results were obtained. The findings are discussed with reference to long lasting potentiation of synaptic activity in the perforant path-dentate granule cell connection.", "PMID": 974790}
{"id": "pubmed23n0032_9975", "title": "Effect of prenatal heat stress on brain growth and serial discrimination reversal learning in the guinea pig.", "content": "Exposure of pregnant guinea pigs to an environmental temperature of 42 degrees C for 1 hr daily on Days 20-24 of gestation resulted in a significant reduction in the birthweight and brainweight of newborn offspring. These deficits persisted to early maturity and were still evident at 250 days of age following behavioral testing. Although some degree of postnatal neurogenesis and brain growth had occurred, this was not sufficient to compensate for the retarding influence of prenatal hyperthermia. Heat stress was also observed to seriously impair learning performance on the original discrimination task and this tendency persisted over the subsequent 4 reversals for both initial and perseverative errors. Impaired learning performance was related to reduction in brainweight. Animals with lower brainweights made significantly more initial, perseverative and total number of errors over the 5 problems. In addition, 12 of the 14 behaviorally tested stressed progeny had brainweights that were at least 2 standard deviations below the mean of the controls and as a result were classified as micrencephalic. Although heat stressed animals showed a significant reduction in the number of amacrine synapses in the inner plexiform layer of the retina, performance differences were not atrributed to changes in synaptic organisation of the retinal circuitry or to visual or other ophthalmic defects, but brain function. Animals with higher mean maternal poststressing core temperatures gave birth to progeny which had smaller whole and part wet-brainweights. Examination of the effect of poststressing core temperature on brainweight revealed that brain growth, independent of bodyweight, was retarded when this temperature elevated above 41.5 degrees C. This represented a rise of approximately 2.1 degrees C above normal and for each 1 degree C rise above this temperature, brainweight was reduced by 0.4227 g of the control value. Analysis of the atmospheric content of the incubator during stressing sessions ruled out the possibility that changes in level of oxygen and carbon dioxide may have contributed to the retarding influence on fetal development and learning ability in the guinea pig.", "contents": "Effect of prenatal heat stress on brain growth and serial discrimination reversal learning in the guinea pig. Exposure of pregnant guinea pigs to an environmental temperature of 42 degrees C for 1 hr daily on Days 20-24 of gestation resulted in a significant reduction in the birthweight and brainweight of newborn offspring. These deficits persisted to early maturity and were still evident at 250 days of age following behavioral testing. Although some degree of postnatal neurogenesis and brain growth had occurred, this was not sufficient to compensate for the retarding influence of prenatal hyperthermia. Heat stress was also observed to seriously impair learning performance on the original discrimination task and this tendency persisted over the subsequent 4 reversals for both initial and perseverative errors. Impaired learning performance was related to reduction in brainweight. Animals with lower brainweights made significantly more initial, perseverative and total number of errors over the 5 problems. In addition, 12 of the 14 behaviorally tested stressed progeny had brainweights that were at least 2 standard deviations below the mean of the controls and as a result were classified as micrencephalic. Although heat stressed animals showed a significant reduction in the number of amacrine synapses in the inner plexiform layer of the retina, performance differences were not atrributed to changes in synaptic organisation of the retinal circuitry or to visual or other ophthalmic defects, but brain function. Animals with higher mean maternal poststressing core temperatures gave birth to progeny which had smaller whole and part wet-brainweights. Examination of the effect of poststressing core temperature on brainweight revealed that brain growth, independent of bodyweight, was retarded when this temperature elevated above 41.5 degrees C. This represented a rise of approximately 2.1 degrees C above normal and for each 1 degree C rise above this temperature, brainweight was reduced by 0.4227 g of the control value. Analysis of the atmospheric content of the incubator during stressing sessions ruled out the possibility that changes in level of oxygen and carbon dioxide may have contributed to the retarding influence on fetal development and learning ability in the guinea pig.", "PMID": 974792}
{"id": "pubmed23n0032_9976", "title": "Nucleus circularis: is it an osmoreceptor in the brain?", "content": "The nucleus circularis, in the anterior hypothalamus, is a group of magnocellular elements arranged in a ring around a capillary bed. The cells are predominantly monopolar, tightly packed, and are flattened at the outer border of the ring. The entire nucleus is surrounded or encapsulated by myelinated fibers. Electrical stimulation of this nucleus produced a short-latency, long-lasting and substantial antidiuresis in ethanol anesthetized rats. Water deprivation induced changes in numbers of nucleoli and cell size increases in these cells. The multiplication of nucleoli in this nucleus during water deprivation was more profound than that previously observed in the supraoptic nucleus. Decreases in multiple nucleoli accompanied voluntary rehydration. Seven criteria for status as an osmoreceptor are listed and the nucleus circularis was found to meet 6 of these criteria, the seventh being the demonstration of receptor potentials which has not yet been attempted.", "contents": "Nucleus circularis: is it an osmoreceptor in the brain? The nucleus circularis, in the anterior hypothalamus, is a group of magnocellular elements arranged in a ring around a capillary bed. The cells are predominantly monopolar, tightly packed, and are flattened at the outer border of the ring. The entire nucleus is surrounded or encapsulated by myelinated fibers. Electrical stimulation of this nucleus produced a short-latency, long-lasting and substantial antidiuresis in ethanol anesthetized rats. Water deprivation induced changes in numbers of nucleoli and cell size increases in these cells. The multiplication of nucleoli in this nucleus during water deprivation was more profound than that previously observed in the supraoptic nucleus. Decreases in multiple nucleoli accompanied voluntary rehydration. Seven criteria for status as an osmoreceptor are listed and the nucleus circularis was found to meet 6 of these criteria, the seventh being the demonstration of receptor potentials which has not yet been attempted.", "PMID": 974791}
{"id": "pubmed23n0032_9977", "title": "Distribution of 3H-alpha-MSH in rat brain.", "content": "Radioactive synthetic alpha-MSH, prepared by selective tritiation of its dibromo-L-tyrosine-derivative, was injected into the carotid artery of intact, hypophysectomized or pinealectomized rats. More radioactivity was found in the occipital cortex, cerebellum, and pons-medulla than in most other brain parts of rats decapitated 15 sec and, to a less significant extent, 30 min after injection. Part of this radioactivity behaved like alpha-MSH in several identification procedures. After administration of 3H-tyrosine, the least radioactivity was consistently found in the pons-medulla. Large amounts of radioactivity after 3H-alpha-MSH were found in the pituitary and, particularly in the hypophysectomized rats, in the pineal. The half-time disappearance of the first component of radioactivity from the blood of each group of rats injected with tritiated-alpha-MSH was no more than 2.5 minutes, a time considerably shorter than the behavioral and EEG effects of this pituitary hormone on visual attention.", "contents": "Distribution of 3H-alpha-MSH in rat brain. Radioactive synthetic alpha-MSH, prepared by selective tritiation of its dibromo-L-tyrosine-derivative, was injected into the carotid artery of intact, hypophysectomized or pinealectomized rats. More radioactivity was found in the occipital cortex, cerebellum, and pons-medulla than in most other brain parts of rats decapitated 15 sec and, to a less significant extent, 30 min after injection. Part of this radioactivity behaved like alpha-MSH in several identification procedures. After administration of 3H-tyrosine, the least radioactivity was consistently found in the pons-medulla. Large amounts of radioactivity after 3H-alpha-MSH were found in the pituitary and, particularly in the hypophysectomized rats, in the pineal. The half-time disappearance of the first component of radioactivity from the blood of each group of rats injected with tritiated-alpha-MSH was no more than 2.5 minutes, a time considerably shorter than the behavioral and EEG effects of this pituitary hormone on visual attention.", "PMID": 974794}
{"id": "pubmed23n0032_9978", "title": "Acetylcholine, an inhibitory transmitter in the rat lateral hypothalamus.", "content": "Basolateral stimulation of the amygdala inhibited spontaneous firing of rat lateral hypothalamic neurons. The inhibition was augmented by eserine and blocked by atropine. Acetylcholine mimicked the inhibition and produced approximately 15 mV of hyperpolarization beyond the original resting membrane potential which stopped spontaneous firing. The ACh effects were reversible. Results indicate that ACh might be an inhibitory transmitter involved in LH inhibition by AL stimulation.", "contents": "Acetylcholine, an inhibitory transmitter in the rat lateral hypothalamus. Basolateral stimulation of the amygdala inhibited spontaneous firing of rat lateral hypothalamic neurons. The inhibition was augmented by eserine and blocked by atropine. Acetylcholine mimicked the inhibition and produced approximately 15 mV of hyperpolarization beyond the original resting membrane potential which stopped spontaneous firing. The ACh effects were reversible. Results indicate that ACh might be an inhibitory transmitter involved in LH inhibition by AL stimulation.", "PMID": 974793}
{"id": "pubmed23n0032_9979", "title": "Evoked potential-unit relationships in behaving cats.", "content": "Adult cats were implanted with a movable microelectrode, and trained to respond differentially to two different frequencies of light flicker. Unit responses and evoked potentials were recorded along trajectories in the visual cortex and lateral geniculate nucleus. Although strong correlations are shown to exist between certain components of the evoked potential and peaks in the poststimulus histogram, it is demonstrated that it is impossible to specify a causal, predictive relationship.", "contents": "Evoked potential-unit relationships in behaving cats. Adult cats were implanted with a movable microelectrode, and trained to respond differentially to two different frequencies of light flicker. Unit responses and evoked potentials were recorded along trajectories in the visual cortex and lateral geniculate nucleus. Although strong correlations are shown to exist between certain components of the evoked potential and peaks in the poststimulus histogram, it is demonstrated that it is impossible to specify a causal, predictive relationship.", "PMID": 974796}
{"id": "pubmed23n0032_9980", "title": "Single cell activity in chronic unit recording: a quantitative study of the unit amplitude spectrum.", "content": "The ratio of standard deviation to mean, of the amplitude spectrum of well resolved single units, is shown to scale exponentially. This scaling is explained on the basis of a consideration of the relationship of extracellular voltage to voltage gradient, in a dendritic neuron model. A method is then advanced to extract, from multi-unit data, normally distributed amplitude spectra which are indistinguishable from those of well resolved single units. This method is suggested as a means of characterizing, in general, the unit activity obtained from chronically implanted preparations.", "contents": "Single cell activity in chronic unit recording: a quantitative study of the unit amplitude spectrum. The ratio of standard deviation to mean, of the amplitude spectrum of well resolved single units, is shown to scale exponentially. This scaling is explained on the basis of a consideration of the relationship of extracellular voltage to voltage gradient, in a dendritic neuron model. A method is then advanced to extract, from multi-unit data, normally distributed amplitude spectra which are indistinguishable from those of well resolved single units. This method is suggested as a means of characterizing, in general, the unit activity obtained from chronically implanted preparations.", "PMID": 974795}
{"id": "pubmed23n0032_9981", "title": "An examination of the participation of neurons in readout from memory.", "content": "Adult cats were implanted with a movable microelectrode and were trained to perform for food reward in response to diffuse light flicker at two different frequencies. After substantial overtraining, the patterns of cell response (poststimulus histogram) were obtained during generalization trials, using an intermediate frequency stimulus. An average of 29% of the cells examined in lateral geniculate nucleus and visual cortex traverses showed statistically significant differences in the late component of the neuronal response when different responses to the same generalization stimulus were compared.", "contents": "An examination of the participation of neurons in readout from memory. Adult cats were implanted with a movable microelectrode and were trained to perform for food reward in response to diffuse light flicker at two different frequencies. After substantial overtraining, the patterns of cell response (poststimulus histogram) were obtained during generalization trials, using an intermediate frequency stimulus. An average of 29% of the cells examined in lateral geniculate nucleus and visual cortex traverses showed statistically significant differences in the late component of the neuronal response when different responses to the same generalization stimulus were compared.", "PMID": 974797}
{"id": "pubmed23n0032_9982", "title": "Computer-determined EEG patterns associated with memory-facilitating drugs and with ECS.", "content": "Cortical EEG patterns of rats were analyzed by computer following administration of analeptic drugs at doses previously shown to facilitate posttrial memory processes. The drugs produced an increase in the power of the EEG primarily by increasing the amount of activity in delta and lower-theta frequency bands. The increase in lower frequency theta was accompanied by a decrease in higher frequency theta, thus increasing the regularity in the theta band. Electroconvulsive shock, which disrupts posttrial memory processes, also increased delta activity, but it greatly lowered amplitudes throughout the EEG frequency spectrum. It is concluded that if any consistent relationship between the cortical EEG and posttrial memory processes exists, then increased amplitude and regularity in the delta and theta bands are associated with increased amounts of memory storage--at least in alert animals. Further studies are required to determine if a causal influence is involved in this correlation.", "contents": "Computer-determined EEG patterns associated with memory-facilitating drugs and with ECS. Cortical EEG patterns of rats were analyzed by computer following administration of analeptic drugs at doses previously shown to facilitate posttrial memory processes. The drugs produced an increase in the power of the EEG primarily by increasing the amount of activity in delta and lower-theta frequency bands. The increase in lower frequency theta was accompanied by a decrease in higher frequency theta, thus increasing the regularity in the theta band. Electroconvulsive shock, which disrupts posttrial memory processes, also increased delta activity, but it greatly lowered amplitudes throughout the EEG frequency spectrum. It is concluded that if any consistent relationship between the cortical EEG and posttrial memory processes exists, then increased amplitude and regularity in the delta and theta bands are associated with increased amounts of memory storage--at least in alert animals. Further studies are required to determine if a causal influence is involved in this correlation.", "PMID": 974799}
{"id": "pubmed23n0032_9983", "title": "Hypertrophy and redistribution of astrocytes in the deafferented dentate gyrus.", "content": "The response of the astroglial population of the dentate gyrus molecular layer to removal of that region's primary afferent was investigated using Cajal's gold sublimate method. Deafferentation caused the astrocytes to hypertrophy, an effect which was detectable at 24 hr and maximal at 72-96 hr post-lesion. Following this, the astroglia entered a lengthy period of gradual atrophy. Counts of the astrocytes in the various sublayers of the molecular layer led to the conclusion that these cells migrate into denervated dendritic areas from neighboring, nondeafferented zones.", "contents": "Hypertrophy and redistribution of astrocytes in the deafferented dentate gyrus. The response of the astroglial population of the dentate gyrus molecular layer to removal of that region's primary afferent was investigated using Cajal's gold sublimate method. Deafferentation caused the astrocytes to hypertrophy, an effect which was detectable at 24 hr and maximal at 72-96 hr post-lesion. Following this, the astroglia entered a lengthy period of gradual atrophy. Counts of the astrocytes in the various sublayers of the molecular layer led to the conclusion that these cells migrate into denervated dendritic areas from neighboring, nondeafferented zones.", "PMID": 974798}
{"id": "pubmed23n0032_9984", "title": "Evidence for independent osmosensitivity of lateral preoptic and lateral hypothalamic neurons.", "content": "The purpose of the present study was to determine if a functional or modulatory relationship exists between osmosensitive neurons of the lateral preoptic (LPO) and lateral hypothalamic (LHA) areas. Unit activity was recorded from one neural area (LPO or LHA) following bilateral destruction of the opposite area, i.e., LPO recording site with LHA lesion site, and vice-versa. Bilateral destruction of the LPO or LHA did not eliminate the osmosensitivity characteristics of LPO or LHA units, demonstrating that units of either area do not require input from the opposite area for osmosensitivity.", "contents": "Evidence for independent osmosensitivity of lateral preoptic and lateral hypothalamic neurons. The purpose of the present study was to determine if a functional or modulatory relationship exists between osmosensitive neurons of the lateral preoptic (LPO) and lateral hypothalamic (LHA) areas. Unit activity was recorded from one neural area (LPO or LHA) following bilateral destruction of the opposite area, i.e., LPO recording site with LHA lesion site, and vice-versa. Bilateral destruction of the LPO or LHA did not eliminate the osmosensitivity characteristics of LPO or LHA units, demonstrating that units of either area do not require input from the opposite area for osmosensitivity.", "PMID": 974803}
{"id": "pubmed23n0032_9985", "title": "Electrical stimulation of the tooth pulp in the study of pain.", "content": "Unit activity was recorded from bulbar neurons in the immobilized, lightly anesthetized cat following bipolar, electrical stimulation of the canine tooth pulp and the immediately proximal gingiva. When feasable, variations in neuronal responding were studied subsequent to the application of a topical anesthetic to the portion of the gingiva being stimulated. The results indicate that (1) stimulation of the pulp with insulated, embedded, dental electrodes does not excite receptors in adjacent, nondental tissue and (2) stimulation of the gingiva through insulated, concentric electrodes does not activate pulpal receptors in near-by teeth. In addition, the results corroborate previous demonstrations that current spread beyond the pulpal cavity is unlikely when appropriate stimulating procedures are used. Together, the data lend support to the position that electrical stimulation of the tooth pulp provides a unique means for the selective, parametric study of a known pain sensor.", "contents": "Electrical stimulation of the tooth pulp in the study of pain. Unit activity was recorded from bulbar neurons in the immobilized, lightly anesthetized cat following bipolar, electrical stimulation of the canine tooth pulp and the immediately proximal gingiva. When feasable, variations in neuronal responding were studied subsequent to the application of a topical anesthetic to the portion of the gingiva being stimulated. The results indicate that (1) stimulation of the pulp with insulated, embedded, dental electrodes does not excite receptors in adjacent, nondental tissue and (2) stimulation of the gingiva through insulated, concentric electrodes does not activate pulpal receptors in near-by teeth. In addition, the results corroborate previous demonstrations that current spread beyond the pulpal cavity is unlikely when appropriate stimulating procedures are used. Together, the data lend support to the position that electrical stimulation of the tooth pulp provides a unique means for the selective, parametric study of a known pain sensor.", "PMID": 974804}
{"id": "pubmed23n0032_9986", "title": "Raphe neurons and barbiturate induced rhythmic activity in the subthalamic nucleus and ventral tegmental area.", "content": "A continuous rhythmic discharge in the subthalamic nucleus (STN), lateral habenular nucleus, and ventral tegmental area (VTA) is associated with tremor in cats lightly anesthetized with barbiturate. The pontine raphe nuclei centralis superior and inferior also show barbiturate dependent slow wave and unitary activity. Stimulation of the raphe region produces evoked potentials and synchronization of the oscillatory activity in STN and VTA. The latency of the evoked response is shortest in STN. Extensive raphe lesions abolish the rhythmic activity in STN and VTA, but lesions which do not completely destroy nuclei centralis superior and inferior are not effective. In single cell recordings 81% of 142 cells in the pontine raphe region and medial reticular formation are inhibited following VTA stimulation. Fifty-four percent of 129 cells in the same region were inhibited when tested with STN stimulation. Forty-seven raphe paramedian reticular formation cells showed convergence of inhibitory input from STN, VTA, and contralateral sciatic nerve (Group II and III fibers). Depletion of serotonin with rho-chlorophenylalanine did not affect the appearance of barbiturate rhythmic activity. It is concluded that the pontine raphe region is not essential for maintenance of the oscillatory activity in STN and VTA, and that the raphe region is on the output side of the system.", "contents": "Raphe neurons and barbiturate induced rhythmic activity in the subthalamic nucleus and ventral tegmental area. A continuous rhythmic discharge in the subthalamic nucleus (STN), lateral habenular nucleus, and ventral tegmental area (VTA) is associated with tremor in cats lightly anesthetized with barbiturate. The pontine raphe nuclei centralis superior and inferior also show barbiturate dependent slow wave and unitary activity. Stimulation of the raphe region produces evoked potentials and synchronization of the oscillatory activity in STN and VTA. The latency of the evoked response is shortest in STN. Extensive raphe lesions abolish the rhythmic activity in STN and VTA, but lesions which do not completely destroy nuclei centralis superior and inferior are not effective. In single cell recordings 81% of 142 cells in the pontine raphe region and medial reticular formation are inhibited following VTA stimulation. Fifty-four percent of 129 cells in the same region were inhibited when tested with STN stimulation. Forty-seven raphe paramedian reticular formation cells showed convergence of inhibitory input from STN, VTA, and contralateral sciatic nerve (Group II and III fibers). Depletion of serotonin with rho-chlorophenylalanine did not affect the appearance of barbiturate rhythmic activity. It is concluded that the pontine raphe region is not essential for maintenance of the oscillatory activity in STN and VTA, and that the raphe region is on the output side of the system.", "PMID": 974800}
{"id": "pubmed23n0032_9987", "title": "Pathologic effects of antimetabolites on the hippocampus of diet controlled mice.", "content": "Marked histological changes are observed in the hippocampi of mice maintained on a niacin deficient diet following a single injection of 3-acetylpyridine (antimetabolite of nicatinamide). Extensive neuronal damage occurs in areas H2, H3 and to a lesser degree in the dentate gyrus. The neurons appear to be shrunken, deeply basophilic and exhibit a loss of intracellular architecture. The neuronal response in pyridoxine deficient mice treated with 4-methoxypyridoxine (antimetabolite of pyridoxine) is not remarkable.", "contents": "Pathologic effects of antimetabolites on the hippocampus of diet controlled mice. Marked histological changes are observed in the hippocampi of mice maintained on a niacin deficient diet following a single injection of 3-acetylpyridine (antimetabolite of nicatinamide). Extensive neuronal damage occurs in areas H2, H3 and to a lesser degree in the dentate gyrus. The neurons appear to be shrunken, deeply basophilic and exhibit a loss of intracellular architecture. The neuronal response in pyridoxine deficient mice treated with 4-methoxypyridoxine (antimetabolite of pyridoxine) is not remarkable.", "PMID": 974805}
{"id": "pubmed23n0032_9988", "title": "Instrumental avoidance conditioning in the spinal rat.", "content": "Spinal rats exposed to an instrumental avoidance routine in a counterbalanced Horridge paradigm were able to achieve successively higher criteria. Both experimental and yoked control animals were capable of instrumental avoidance conditioning to incremental criteria; experimental animals exhibited retention of the task when tested. During acquisition, naive experimental animals were superior in performance to previous control animals. Due to the use of a counterbalanced Horridge paradigm, the effectsof sensitization and response variability are probably not sufficient to explain all of the results of this experiment. The data suggest that both graded acquisition and retention occur at the spinal level.", "contents": "Instrumental avoidance conditioning in the spinal rat. Spinal rats exposed to an instrumental avoidance routine in a counterbalanced Horridge paradigm were able to achieve successively higher criteria. Both experimental and yoked control animals were capable of instrumental avoidance conditioning to incremental criteria; experimental animals exhibited retention of the task when tested. During acquisition, naive experimental animals were superior in performance to previous control animals. Due to the use of a counterbalanced Horridge paradigm, the effectsof sensitization and response variability are probably not sufficient to explain all of the results of this experiment. The data suggest that both graded acquisition and retention occur at the spinal level.", "PMID": 974801}
{"id": "pubmed23n0032_9989", "title": "A theory of the serial lesion effect.", "content": "The serial lesion effect is explained as a result of differentiation contingent upon induction by a spared residue. The induction can only take place when the lesion is made since the intact structures repress differentiation when some critical mass is reached. A distinction between the process giving rise to reorganization and reorganization as an outcome is made.", "contents": "A theory of the serial lesion effect. The serial lesion effect is explained as a result of differentiation contingent upon induction by a spared residue. The induction can only take place when the lesion is made since the intact structures repress differentiation when some critical mass is reached. A distinction between the process giving rise to reorganization and reorganization as an outcome is made.", "PMID": 974806}
{"id": "pubmed23n0032_9990", "title": "Superposition of binaural influences on single neuron activity in the medial superior olive elicited by electrical stimulation of the osseous spiral laminae.", "content": "The single unit response of medial superior olive (accessory nucleus) neurons was investigated, when elicited by electrical stimulation of nervous processes in the osseous spiral laminae in both cochleae and also by antidromic stimulation from the inferior colliculi. The osseous spiral laminae were stimulated at the first, second or third turns with grounding of one of the unstimulated turns. Time of signal arrival differences (delta tau) at the two cochleae and also intensity differences (deltai) were varied. (2) It is concluded that (A) binaural interaction in the MSO is not based on totally excitatory or inhibitory influences from either ear, but on cycles of excitation and inhibition; (B) these influences approximately obey the laws of superposition, i.e., the influences add linearly to determine discharge. (3) It is demonstrated that: (a) electrical stimulation, unlike acoustical stimulation, elicits a regular neural response; (b) the neural response functions elicited by changes in delta tau are deltai exhibit maxima and minima and are not smooth functions; (c) fine latency changes in the neural response are correlated with changes in delta tau and deltai; (d) the stimulation of different turns of the osseous spiral laminae (producing different current flows) elicits a different neural response depending on the turn stimulated, an effect reflecting the anatomical tonotopic arrangement of the medial superior olive; (e) changes in the stimulus differences delta tau and deltai are correlated with the ratio of the spike count in a short period following the stimulus, to the spike count in a longer total period; (f) changes in the stimulus differences delta tau and deltai elicit a neural response which varies according to the turn stimulated.", "contents": "Superposition of binaural influences on single neuron activity in the medial superior olive elicited by electrical stimulation of the osseous spiral laminae. The single unit response of medial superior olive (accessory nucleus) neurons was investigated, when elicited by electrical stimulation of nervous processes in the osseous spiral laminae in both cochleae and also by antidromic stimulation from the inferior colliculi. The osseous spiral laminae were stimulated at the first, second or third turns with grounding of one of the unstimulated turns. Time of signal arrival differences (delta tau) at the two cochleae and also intensity differences (deltai) were varied. (2) It is concluded that (A) binaural interaction in the MSO is not based on totally excitatory or inhibitory influences from either ear, but on cycles of excitation and inhibition; (B) these influences approximately obey the laws of superposition, i.e., the influences add linearly to determine discharge. (3) It is demonstrated that: (a) electrical stimulation, unlike acoustical stimulation, elicits a regular neural response; (b) the neural response functions elicited by changes in delta tau are deltai exhibit maxima and minima and are not smooth functions; (c) fine latency changes in the neural response are correlated with changes in delta tau and deltai; (d) the stimulation of different turns of the osseous spiral laminae (producing different current flows) elicits a different neural response depending on the turn stimulated, an effect reflecting the anatomical tonotopic arrangement of the medial superior olive; (e) changes in the stimulus differences delta tau and deltai are correlated with the ratio of the spike count in a short period following the stimulus, to the spike count in a longer total period; (f) changes in the stimulus differences delta tau and deltai elicit a neural response which varies according to the turn stimulated.", "PMID": 974802}
{"id": "pubmed23n0032_9991", "title": "Effect of raphe lesions on brain serotonin in the cat.", "content": "A quantitative study of the regional distribution of serotonin (5-HT) in the forebrain, was performed in adult cats, following brainstem lesions. Seven to 10 days survival times were used to avoid nonspecific variations of the amine levels, as were observed in chronic preparations. Significant decreases of 5-HT levels were found after lesions of the nucleus centralis superior (CS), in hypothalamus, preoptic area, amygdala and hippocampus. After lesions of the nucleus dorsalis raphe (Dr), significant 5-HT decreases were also found in the hypothalamus and hippocampus. There was no change of 5-HT levels after lesions placed in the lateral midbrain, pontine tegmentum, or caudal pontine raphe, contrary to that was reported for chronic lesioned cats. These results suggest that CS and Dr may integrate different serotonergic subsystems and, probably, each one of these subsystems is related to specific functional phenomena.", "contents": "Effect of raphe lesions on brain serotonin in the cat. A quantitative study of the regional distribution of serotonin (5-HT) in the forebrain, was performed in adult cats, following brainstem lesions. Seven to 10 days survival times were used to avoid nonspecific variations of the amine levels, as were observed in chronic preparations. Significant decreases of 5-HT levels were found after lesions of the nucleus centralis superior (CS), in hypothalamus, preoptic area, amygdala and hippocampus. After lesions of the nucleus dorsalis raphe (Dr), significant 5-HT decreases were also found in the hypothalamus and hippocampus. There was no change of 5-HT levels after lesions placed in the lateral midbrain, pontine tegmentum, or caudal pontine raphe, contrary to that was reported for chronic lesioned cats. These results suggest that CS and Dr may integrate different serotonergic subsystems and, probably, each one of these subsystems is related to specific functional phenomena.", "PMID": 974808}
{"id": "pubmed23n0032_9992", "title": "Hypothalmic influences on the electrical activity of the olfactory pathway.", "content": "By means of evoked potentials a direct efferent connection was found to run from the posterior hypothalmus and medial forebrain bundle to primary olfactory structures (olfactory bulb, olfactory tubercle and prepyriform cortex). The pathway from the hypothalmus to the olfactory bulb follows in the lateral olfactory tract at a conduction velocity 5-10 m/sec. The olfactory tubercle functions as a relay station for the efferent fibers from various sources, running to the olfactory bulb. In animals with electrodes chronically implanted in the olfactory structures, hypothalamic stimulation gives rise to a prolonged train of hypersynchronous bursts of activity (40-50 Hz), which resemble the arousal reaction. This response is modified by transecting the cervical sympathetic trunk. By pathways still to be defined, potentials are evoked in the olfactory bulb by stimulation of the cervical sympathetic trunk and the termination of these sympathetic fibers shows a common postsynaptic neuronal pool with axons of hyopothalmic origin. Epinephrine topically applied to the olfactory mucosa induced hypersynchronous activity in olfactory structures, quite similar to that consequent to hypothalmic stimulation. These results suggest a multichanneled hypothalmic modulation of olfactory input.", "contents": "Hypothalmic influences on the electrical activity of the olfactory pathway. By means of evoked potentials a direct efferent connection was found to run from the posterior hypothalmus and medial forebrain bundle to primary olfactory structures (olfactory bulb, olfactory tubercle and prepyriform cortex). The pathway from the hypothalmus to the olfactory bulb follows in the lateral olfactory tract at a conduction velocity 5-10 m/sec. The olfactory tubercle functions as a relay station for the efferent fibers from various sources, running to the olfactory bulb. In animals with electrodes chronically implanted in the olfactory structures, hypothalamic stimulation gives rise to a prolonged train of hypersynchronous bursts of activity (40-50 Hz), which resemble the arousal reaction. This response is modified by transecting the cervical sympathetic trunk. By pathways still to be defined, potentials are evoked in the olfactory bulb by stimulation of the cervical sympathetic trunk and the termination of these sympathetic fibers shows a common postsynaptic neuronal pool with axons of hyopothalmic origin. Epinephrine topically applied to the olfactory mucosa induced hypersynchronous activity in olfactory structures, quite similar to that consequent to hypothalmic stimulation. These results suggest a multichanneled hypothalmic modulation of olfactory input.", "PMID": 974807}
{"id": "pubmed23n0032_9993", "title": "Drug effects on sucessive discrimination learning in young chickens.", "content": "Chicks were trained to avoid pecking either a red or a blue bead in a one-trial avoidance task by coating one bead with methy anthranilate. They avoided the aversant bead on retention tests 10 to 180 min or 24 hr after learning, but not the neutral bead. Intracranial administration of ouabain or cycloheximide (CXM) 5 min before learning resulted in decay in retention after 10 and 30 min respectively following learning, discrimination being effective prior to those times. In a second experiment, chicks were trained on three physically distinct beads, two of which were made aversive during the learning period, the training trials separated by an hour. Saline-treated chickens retained memory of both aversive beads on retention trials 180 min later. CXM- and ouabain-treated chickens showed loss of memory for the bead associated with the drug but showed retention of the task which was not associated with the drug.", "contents": "Drug effects on sucessive discrimination learning in young chickens. Chicks were trained to avoid pecking either a red or a blue bead in a one-trial avoidance task by coating one bead with methy anthranilate. They avoided the aversant bead on retention tests 10 to 180 min or 24 hr after learning, but not the neutral bead. Intracranial administration of ouabain or cycloheximide (CXM) 5 min before learning resulted in decay in retention after 10 and 30 min respectively following learning, discrimination being effective prior to those times. In a second experiment, chicks were trained on three physically distinct beads, two of which were made aversive during the learning period, the training trials separated by an hour. Saline-treated chickens retained memory of both aversive beads on retention trials 180 min later. CXM- and ouabain-treated chickens showed loss of memory for the bead associated with the drug but showed retention of the task which was not associated with the drug.", "PMID": 974809}
{"id": "pubmed23n0032_9994", "title": "Hypothalamic Na+ and Ca++ ions and temperature set-point: new mechanisms of action of a central or peripheral thermal challenge and intrahypothalamic 5-HT, NE, PGEi and pyrogen.", "content": "The effects of changes in ambient and central temperature, amines, PGEu and pyrogen were investigated with respect to the mechanism of Na+-Ca++ ratio in the posterior hypothalamus of the unrestrained cat. Guide tubes were implanted bilaterally above the posterior hypothalamic area of 23 cats so as to accommodate push-pull cannulae. After a Na+ or Ca++ sensitive site was identified by perfusion at 50 mul/min of an artificial CSF containing 10.4 mM excess Ca++ ions or 13.6 mM excess Na+ ions, several types of experiments were undertaken with the results summarized as follows: if the cat was exposed to a cold or warm environmental temperature as the posterior hypothalamus was perfused with excess cation, the typical hypothermia was produced by Ca++ and hyperthermia by Na+ ions. However, if the cat was exposed to peripheral cooling or warming 30 min prior to the perfusion, the fall or rise produced by Ca++ or Na+ was attenuated or prevented. In other experiments, 1.0 muCi 45Ca++ was injected in the ion sensitive site in the posterior hypothalamus to label stores of the cation. Raising of ambient temperature caused a retention of 45Ca++ in this hypothalmic area, whereas a cold environmental temperature enhanced the efflux of 45Ca++ at the same perfusion site. The magnitude of change in 45Ca++ efflux depended upon the intensity of the thermal challenge. Similarly, warming of the anterior hypothalmic, preoptic area by means of implanted thermodes caused an immediate diminution in 45Ca++ efflux in the posterior hypothalamus, whereas cooling of this anterior region augmented the extrusion of 45Ca++ ions from the posterior area. When substances which produce a temperature change were applied to the same thermosensitive zone, the direction of shift in 45Ca++ flux in the posterior area corresponded to the signal for heat production or heat loss. That is, the microinjection of 5-HT, PGE1 or Salmonella typhosa into the anterior hypothalamus enhanced the efflux of 45Ca++ in the posterior hypothalamus as hyperthermia developed, whereas a similar microinjection of norepinephrine reduced the 45Ca++ output from the same sites. Finally, locally anesthetizing the cells of the anterior hypothalamus by the nerve blocker, procaine, prevented the cold and heat-induced 45Ca++ eflux and retention, respectively. These results suggest that if the Na+-Ca++ ratio in the posterior hypothalamus establishes and maintains the set-point for body temperature of 37 degrees -38 degrees C, the mechanism of lability of Ca++ through changes in binding characteristics, transport, or metabolism of the cation serves two purposes: (1) the active defense of the set-point temperature through gradations in ion shifts; and (2) the upward or downward change in set-point value, pathological or normal, triggered by virtue of impulses relayed from the anterior hypothalamus.", "contents": "Hypothalamic Na+ and Ca++ ions and temperature set-point: new mechanisms of action of a central or peripheral thermal challenge and intrahypothalamic 5-HT, NE, PGEi and pyrogen. The effects of changes in ambient and central temperature, amines, PGEu and pyrogen were investigated with respect to the mechanism of Na+-Ca++ ratio in the posterior hypothalamus of the unrestrained cat. Guide tubes were implanted bilaterally above the posterior hypothalamic area of 23 cats so as to accommodate push-pull cannulae. After a Na+ or Ca++ sensitive site was identified by perfusion at 50 mul/min of an artificial CSF containing 10.4 mM excess Ca++ ions or 13.6 mM excess Na+ ions, several types of experiments were undertaken with the results summarized as follows: if the cat was exposed to a cold or warm environmental temperature as the posterior hypothalamus was perfused with excess cation, the typical hypothermia was produced by Ca++ and hyperthermia by Na+ ions. However, if the cat was exposed to peripheral cooling or warming 30 min prior to the perfusion, the fall or rise produced by Ca++ or Na+ was attenuated or prevented. In other experiments, 1.0 muCi 45Ca++ was injected in the ion sensitive site in the posterior hypothalamus to label stores of the cation. Raising of ambient temperature caused a retention of 45Ca++ in this hypothalmic area, whereas a cold environmental temperature enhanced the efflux of 45Ca++ at the same perfusion site. The magnitude of change in 45Ca++ efflux depended upon the intensity of the thermal challenge. Similarly, warming of the anterior hypothalmic, preoptic area by means of implanted thermodes caused an immediate diminution in 45Ca++ efflux in the posterior hypothalamus, whereas cooling of this anterior region augmented the extrusion of 45Ca++ ions from the posterior area. When substances which produce a temperature change were applied to the same thermosensitive zone, the direction of shift in 45Ca++ flux in the posterior area corresponded to the signal for heat production or heat loss. That is, the microinjection of 5-HT, PGE1 or Salmonella typhosa into the anterior hypothalamus enhanced the efflux of 45Ca++ in the posterior hypothalamus as hyperthermia developed, whereas a similar microinjection of norepinephrine reduced the 45Ca++ output from the same sites. Finally, locally anesthetizing the cells of the anterior hypothalamus by the nerve blocker, procaine, prevented the cold and heat-induced 45Ca++ eflux and retention, respectively. These results suggest that if the Na+-Ca++ ratio in the posterior hypothalamus establishes and maintains the set-point for body temperature of 37 degrees -38 degrees C, the mechanism of lability of Ca++ through changes in binding characteristics, transport, or metabolism of the cation serves two purposes: (1) the active defense of the set-point temperature through gradations in ion shifts; and (2) the upward or downward change in set-point value, pathological or normal, triggered by virtue of impulses relayed from the anterior hypothalamus.", "PMID": 974810}
{"id": "pubmed23n0032_9995", "title": "Peptides from pigeon cerebrum: two-dimensional fingerprinting and amino acid analysis.", "content": "The number and heterogeneity of endogenous peptides from pigeon cerebrum were studied by two-dimensional peptide fingerprinting. A highly consistent pattern of 8-9 peptides in the 1000-5000 molecular weight range was obtained from each cerebral sample. Amino acid analysis of the peptide material indicated that 30% of the identified residues were dicarboxylic amino acids and 30% were alphatic amino acids. There was no evidence for the presence of arginine or half-cystine in the peptide fraction.", "contents": "Peptides from pigeon cerebrum: two-dimensional fingerprinting and amino acid analysis. The number and heterogeneity of endogenous peptides from pigeon cerebrum were studied by two-dimensional peptide fingerprinting. A highly consistent pattern of 8-9 peptides in the 1000-5000 molecular weight range was obtained from each cerebral sample. Amino acid analysis of the peptide material indicated that 30% of the identified residues were dicarboxylic amino acids and 30% were alphatic amino acids. There was no evidence for the presence of arginine or half-cystine in the peptide fraction.", "PMID": 974812}
{"id": "pubmed23n0032_9996", "title": "Paraventricular nucleus: changes in the medial and lateral cell groups during dehydration and rehydration in the rat.", "content": "Cell area, percentage of cells with more than one nucleolus, and percentage of cells with the nucleolus apposed to the nuclear membrane (marginated) were compared in the medial and lateral magnocellular portions of the paraventricular nucleus (PVN) during water deprivation and voluntary rehydration. Lateral cells were larger and had a greater percentage of cells with multiple nucleoli in all conditions of deprivation and rehydration. In addition laterals were found to have more cells with marginated nucleoli in these cells with only one nucleoli. Medial and lateral cells showed similar responses to deprivation and rehydration.", "contents": "Paraventricular nucleus: changes in the medial and lateral cell groups during dehydration and rehydration in the rat. Cell area, percentage of cells with more than one nucleolus, and percentage of cells with the nucleolus apposed to the nuclear membrane (marginated) were compared in the medial and lateral magnocellular portions of the paraventricular nucleus (PVN) during water deprivation and voluntary rehydration. Lateral cells were larger and had a greater percentage of cells with multiple nucleoli in all conditions of deprivation and rehydration. In addition laterals were found to have more cells with marginated nucleoli in these cells with only one nucleoli. Medial and lateral cells showed similar responses to deprivation and rehydration.", "PMID": 974811}
{"id": "pubmed23n0032_9997", "title": "Calcium inhibits catecholamine depletion by reserpine from carotid body glomus cells.", "content": "Histofluorescent and quantitative microfluorimetric studies have been performed on glomus cells of the rat carotid body, which fluoresce intently after treatment by paraformaldehyde vapor. Reserpine causes a reduction in intensity of fluorescence of about 90%. Subcutaneous injections of calcium chloride (100-300 mg/kg) were given before reserpine. Calcium prevents the depletion of catecholamies from the glomus cells by reserpine. This effect is dose related in that the depletion of catecholamines by heavy reserpine doses (15 mg/kg) cannot be overcome by calcium, medium doses (5 mg/kg) can be overcome but variably, and light doses (1 mg/kg) are always overcome substantially and can result in virtually complete inhibition of the depletion by reserpine. If calcium might counteract the effects of reserpine by occupying attachment sites of the vesicular membrane, thereby preventing reserpine from reaching its site of action, resulting in the usual uptake of catecholamines by the vesicles and suppression of the depleting action of reserpine.", "contents": "Calcium inhibits catecholamine depletion by reserpine from carotid body glomus cells. Histofluorescent and quantitative microfluorimetric studies have been performed on glomus cells of the rat carotid body, which fluoresce intently after treatment by paraformaldehyde vapor. Reserpine causes a reduction in intensity of fluorescence of about 90%. Subcutaneous injections of calcium chloride (100-300 mg/kg) were given before reserpine. Calcium prevents the depletion of catecholamies from the glomus cells by reserpine. This effect is dose related in that the depletion of catecholamines by heavy reserpine doses (15 mg/kg) cannot be overcome by calcium, medium doses (5 mg/kg) can be overcome but variably, and light doses (1 mg/kg) are always overcome substantially and can result in virtually complete inhibition of the depletion by reserpine. If calcium might counteract the effects of reserpine by occupying attachment sites of the vesicular membrane, thereby preventing reserpine from reaching its site of action, resulting in the usual uptake of catecholamines by the vesicles and suppression of the depleting action of reserpine.", "PMID": 974814}
{"id": "pubmed23n0032_9998", "title": "Effect of visual stimulation on the evoked response recorded at the vertex.", "content": "Previous research on the visual evoked brain response (VEBR) recorded from the occipital scalp indicates that this response shows a high degree of stimulus control. An evoked brain response can also be elicited simultaneously from the vertex response is affected to the same degree by several different aspects of visual stimuli as is the corresponding occipital response. The VEBR was recorded from the vertex of 3 human subjects as a function of changes in stimulus size and intensity. The results showed that the amplitude of the first major positive component of the VEBR was not reliably related to the stimulus parameters. It was concluded that the VEBR recorded at the vertex probably has its source in a distinct cortical generator which is not specifically related to the parameters of visual stimulation.", "contents": "Effect of visual stimulation on the evoked response recorded at the vertex. Previous research on the visual evoked brain response (VEBR) recorded from the occipital scalp indicates that this response shows a high degree of stimulus control. An evoked brain response can also be elicited simultaneously from the vertex response is affected to the same degree by several different aspects of visual stimuli as is the corresponding occipital response. The VEBR was recorded from the vertex of 3 human subjects as a function of changes in stimulus size and intensity. The results showed that the amplitude of the first major positive component of the VEBR was not reliably related to the stimulus parameters. It was concluded that the VEBR recorded at the vertex probably has its source in a distinct cortical generator which is not specifically related to the parameters of visual stimulation.", "PMID": 974813}
{"id": "pubmed23n0032_9999", "title": "\"Twin peaks\" papilledema: the appearance of papilledema with optic tract atrophy.", "content": "A woman with a right middle fossa meningioma causing right optic tract atrophy and papilledema had distinctive funduscopic changes. The ipsilateral eye showed temporal disc pallor and nasal edema. The contralateral disc showed edema of the upper and lower poles which was separated by a horizontal band of optic atrophy. The fluorescein angiographic changes clearly illustrate this pattern. Apart from its theoretical interest this appearance of papilledema and atrophy provides definite localizing information.", "contents": "\"Twin peaks\" papilledema: the appearance of papilledema with optic tract atrophy. A woman with a right middle fossa meningioma causing right optic tract atrophy and papilledema had distinctive funduscopic changes. The ipsilateral eye showed temporal disc pallor and nasal edema. The contralateral disc showed edema of the upper and lower poles which was separated by a horizontal band of optic atrophy. The fluorescein angiographic changes clearly illustrate this pattern. Apart from its theoretical interest this appearance of papilledema and atrophy provides definite localizing information.", "PMID": 974850}
{"id": "pubmed23n0032_10000", "title": "Pheochromocytoma and von Hippel-Lindau's disease.", "content": "Two families with von Hippel-Lindau's disease with associated pheochromocytoma are presented. We include a review of the literature on this association, and discuss its significance for the ophthalmologist.", "contents": "Pheochromocytoma and von Hippel-Lindau's disease. Two families with von Hippel-Lindau's disease with associated pheochromocytoma are presented. We include a review of the literature on this association, and discuss its significance for the ophthalmologist.", "PMID": 974851}
{"id": "pubmed23n0032_10001", "title": "Ophthalmic manifestations of multiple endocrine neoplasia, type three.", "content": "We report the case of a patient with multiple endocrine neoplasia type three, which was first diagnosed by members of the department of Ophthalmology. He was found to have multiple mucosal neuromata and medullary thyroid carcinoma. The ocular components of this syndrome include visible corneal nerves, conjunctival neuromas, thickened lids, anterior displacement of the cilia and a \"dry eye\" syndrome. The major systemic components are medullary thyroid carcinoma and pheochromocytoma. It is important that the Ophthalmologist recognize the ophthalmic manifestations of this syndrome so that early diagnosis and treatment may be possible.", "contents": "Ophthalmic manifestations of multiple endocrine neoplasia, type three. We report the case of a patient with multiple endocrine neoplasia type three, which was first diagnosed by members of the department of Ophthalmology. He was found to have multiple mucosal neuromata and medullary thyroid carcinoma. The ocular components of this syndrome include visible corneal nerves, conjunctival neuromas, thickened lids, anterior displacement of the cilia and a \"dry eye\" syndrome. The major systemic components are medullary thyroid carcinoma and pheochromocytoma. It is important that the Ophthalmologist recognize the ophthalmic manifestations of this syndrome so that early diagnosis and treatment may be possible.", "PMID": 974852}
{"id": "pubmed23n0032_10002", "title": "Tonic pupil: a simple screening test.", "content": "The tonic pupil has a typical appearance and characteristic reactions to both light and the near reflex. Testing with Mecholyl (methacholine) is not possible since the drug is no longer manufactured. The reactions of a group of 25 patients with tonic pupil were studied using dilute pilocarpine, and compared with the pupil responses of a separate control group. We found that concentrations of 0.2% pilocarpine produced too many false-positive reactions in the control group and that 0.05% pilocarpine produced an insufficient response. The 0.1% concentration seemed suitable for ordinary clinical examinations and is recommended for pharmacologic confirmation of the diagnosis of tonic (Adie's) pupil.", "contents": "Tonic pupil: a simple screening test. The tonic pupil has a typical appearance and characteristic reactions to both light and the near reflex. Testing with Mecholyl (methacholine) is not possible since the drug is no longer manufactured. The reactions of a group of 25 patients with tonic pupil were studied using dilute pilocarpine, and compared with the pupil responses of a separate control group. We found that concentrations of 0.2% pilocarpine produced too many false-positive reactions in the control group and that 0.05% pilocarpine produced an insufficient response. The 0.1% concentration seemed suitable for ordinary clinical examinations and is recommended for pharmacologic confirmation of the diagnosis of tonic (Adie's) pupil.", "PMID": 974853}
{"id": "pubmed23n0032_10003", "title": "Scanning electron microscopy of normal and lased rabbit retina.", "content": "This study presents the topography as seen by scanning electron microscopy of the rabbit retina in general and the photoreceptors in particular; and of large laser lesions in the retina.", "contents": "Scanning electron microscopy of normal and lased rabbit retina. This study presents the topography as seen by scanning electron microscopy of the rabbit retina in general and the photoreceptors in particular; and of large laser lesions in the retina.", "PMID": 974854}
{"id": "pubmed23n0032_10004", "title": "The use of bank sclera to correct lid retraction.", "content": "Twenty-two scleral grafts were inserted in patients with retracted lids, the youngest patient was two years old at time of surgery. Two patients were operated on under local anaesthesia and the remainder under general anaesthesia. In the patients with thyroid disease larger grafts were required, the temporal side of the graft being 3 mm greater vertically than the nasal. Our first cases were undercorrected, so we used grafts of a size at least twice that required to lower the lid. Other complications were rare. For ptosis overcorrections, and congenital lid retraction, the graft should be 2 mm bigger horizontally and vertically than the amount required to lower the lid.", "contents": "The use of bank sclera to correct lid retraction. Twenty-two scleral grafts were inserted in patients with retracted lids, the youngest patient was two years old at time of surgery. Two patients were operated on under local anaesthesia and the remainder under general anaesthesia. In the patients with thyroid disease larger grafts were required, the temporal side of the graft being 3 mm greater vertically than the nasal. Our first cases were undercorrected, so we used grafts of a size at least twice that required to lower the lid. Other complications were rare. For ptosis overcorrections, and congenital lid retraction, the graft should be 2 mm bigger horizontally and vertically than the amount required to lower the lid.", "PMID": 974855}
{"id": "pubmed23n0032_10005", "title": "Lactic acid in human aqueous humour.", "content": "We wished to assess the contribution of the lens to the maintenance of the high level of lactic acid found in human aqueous humour. The lactic acid in aqueous humour was estimated by Pryce's modification of the Barker-Summerson method in 41 subjects who were divided into four groups: those with early cataract, those with advanced nuclear cataract, those with mature cataract and those with hypermature (morgagnian type) cataract. Statistical evaluation of the results showed that there was no significant difference in the lactic acid level between the four groups. We assume that the lens does not contribute significantly to the high concentration of lactate found in human aqueous humour.", "contents": "Lactic acid in human aqueous humour. We wished to assess the contribution of the lens to the maintenance of the high level of lactic acid found in human aqueous humour. The lactic acid in aqueous humour was estimated by Pryce's modification of the Barker-Summerson method in 41 subjects who were divided into four groups: those with early cataract, those with advanced nuclear cataract, those with mature cataract and those with hypermature (morgagnian type) cataract. Statistical evaluation of the results showed that there was no significant difference in the lactic acid level between the four groups. We assume that the lens does not contribute significantly to the high concentration of lactate found in human aqueous humour.", "PMID": 974856}
{"id": "pubmed23n0032_10006", "title": "Monocular elevator paresis, Argyll Robertson pupils and sarcoidosis.", "content": "A patient with sarcoidosis involving the central nervous system is described who developed monocular paralysis of elevation and Argyll Robertson pupils. A schema for the control system for vertical eye movements is presented as well as an explanation for monocular elevator palsy. The postulated lesion for monocular elevator palsy could well produce at the same time Argyll Robertson pupils and the cause of this papillary abnormality is discussed.", "contents": "Monocular elevator paresis, Argyll Robertson pupils and sarcoidosis. A patient with sarcoidosis involving the central nervous system is described who developed monocular paralysis of elevation and Argyll Robertson pupils. A schema for the control system for vertical eye movements is presented as well as an explanation for monocular elevator palsy. The postulated lesion for monocular elevator palsy could well produce at the same time Argyll Robertson pupils and the cause of this papillary abnormality is discussed.", "PMID": 974857}
{"id": "pubmed23n0032_10007", "title": "A 'black cornea' secondary to topical epinephrine.", "content": "We report a case of 'black cornea\" secondary to topical epinephrine therapy and present the biochemical analysis of the lesion. The formed pigment is a melanin and not the intermediate substance, adrenochrome, as has been previously reported. The pathogenesis of melanin deposition is reviewed and a hypothesis of epinephrine-melanin binding is suggested.", "contents": "A 'black cornea' secondary to topical epinephrine. We report a case of 'black cornea\" secondary to topical epinephrine therapy and present the biochemical analysis of the lesion. The formed pigment is a melanin and not the intermediate substance, adrenochrome, as has been previously reported. The pathogenesis of melanin deposition is reviewed and a hypothesis of epinephrine-melanin binding is suggested.", "PMID": 974858}
{"id": "pubmed23n0032_10008", "title": "Lack of a significant protective effect of augmented circulating glucose on the ischemic myocardium.BJ.", "content": "Infusion of glucose alone or glucose with insulin in cats subjected to acute myocardial ischemia did not alter the hemodynamic response of the cats to coronary artery ligation. Furthermore, determination of myocardial creatine phosphokinase (CPK) (ATP:creatine N-phosphotransferase, EC 2.7.3.2) activities failed to reveal a protective effect of glucose and insulin upon the status of the developing infarct in the ischemic myocardium. However, glucose and insulin apparently promote clearance of CPK from the plasma and inhibit proteolysis during the early phase of myocardial ischemia. These actions may be of value in generalized adaptive response of the animal to the stress of ischemia, but does not per se appear to diminish the spread of the ischemic damage within the heart not to limit the extension of the evolving infarct.", "contents": "Lack of a significant protective effect of augmented circulating glucose on the ischemic myocardium.BJ. Infusion of glucose alone or glucose with insulin in cats subjected to acute myocardial ischemia did not alter the hemodynamic response of the cats to coronary artery ligation. Furthermore, determination of myocardial creatine phosphokinase (CPK) (ATP:creatine N-phosphotransferase, EC 2.7.3.2) activities failed to reveal a protective effect of glucose and insulin upon the status of the developing infarct in the ischemic myocardium. However, glucose and insulin apparently promote clearance of CPK from the plasma and inhibit proteolysis during the early phase of myocardial ischemia. These actions may be of value in generalized adaptive response of the animal to the stress of ischemia, but does not per se appear to diminish the spread of the ischemic damage within the heart not to limit the extension of the evolving infarct.", "PMID": 974870}
{"id": "pubmed23n0032_10009", "title": "Induced rouleaux formation in interspecies populations of red cells.", "content": "A study was made of rouleaux formation between erythrocytes of different species when suspended in Ringer solution with polyvinylpyrrolidone added (PVP with M.W 360 000, 4.0 G/L). PVP was shown previously to be a suitable asymmetric macromolecule for promoting rouleaux formation. For the present study fresh samples of blood were obtained from humans, cats, rats, mice, dogs, rabbits and guinea pigs. Initally homogenous populations of cells were allowed to form rouleaux in the microscope chamber for the purpose of determining the average cellular dimensions for each species. Subsequently red cells from two species at a time were mixed in equal proportions to assess the degree of preference for the cells of one species to form rouleaux among themselves rather than with cells of the other species. The sequencing of the cells in the mixed rouleaux which formed on the coverslip was determined from photomicrographs, using the previously determined knowledge of the cellular dimensions. Although the visual impression was that the rouleaux were composed of a random selection of cells from the mixed population, a preferencnstrated statisically in nine of the ten combinations tested. Only the rat-mouse mixture of cells was excepted, perhaps because these animals are closely related members of the same family.", "contents": "Induced rouleaux formation in interspecies populations of red cells. A study was made of rouleaux formation between erythrocytes of different species when suspended in Ringer solution with polyvinylpyrrolidone added (PVP with M.W 360 000, 4.0 G/L). PVP was shown previously to be a suitable asymmetric macromolecule for promoting rouleaux formation. For the present study fresh samples of blood were obtained from humans, cats, rats, mice, dogs, rabbits and guinea pigs. Initally homogenous populations of cells were allowed to form rouleaux in the microscope chamber for the purpose of determining the average cellular dimensions for each species. Subsequently red cells from two species at a time were mixed in equal proportions to assess the degree of preference for the cells of one species to form rouleaux among themselves rather than with cells of the other species. The sequencing of the cells in the mixed rouleaux which formed on the coverslip was determined from photomicrographs, using the previously determined knowledge of the cellular dimensions. Although the visual impression was that the rouleaux were composed of a random selection of cells from the mixed population, a preferencnstrated statisically in nine of the ten combinations tested. Only the rat-mouse mixture of cells was excepted, perhaps because these animals are closely related members of the same family.", "PMID": 974871}
{"id": "pubmed23n0032_10010", "title": "Tonin activity in rat saliva: effect of sympathomimetic and parasympathomimetic drugs.", "content": "The effects of carbachol, propranolol and isoproterenol on tonin concentrations in rat saliva have been investigated. The results suggest that the activity of this enzyme in rat saliva is dependent on activity of beta-adrenergic receptors.", "contents": "Tonin activity in rat saliva: effect of sympathomimetic and parasympathomimetic drugs. The effects of carbachol, propranolol and isoproterenol on tonin concentrations in rat saliva have been investigated. The results suggest that the activity of this enzyme in rat saliva is dependent on activity of beta-adrenergic receptors.", "PMID": 974872}
{"id": "pubmed23n0032_10011", "title": "The electrophysiological basis of the motor inhibitory effect of adrenaline on rabbit small intestinal smooth muscle.", "content": "The spontaneous electrical activity of small intestinal smooth muscle cells consists of repetive depolarizations (control activity) on which spikes (response activity) may be superimposed; each spike is preceded by a prepotential. Response activity is associated with contractions. Adrenaline initially abolished the response activity and any prepotenials, as well as the contractions, without altering the control activity or the membrane potential. This effect was followed by hyperpolarization and slight increase in the control potential amplitude. The hyperpolarization was insenstitive tto temperature (Q10 = 1.01) and was larger when the membrane was initially depolarized by K withdrawal but did not occur after the membrane was hyperpolarized by replacing C1 by propionate or by prolonged K withdrawal. It is suggested that adrenaline inhibits intestinal motility by uncoupling the control activity to response activity through suppression of the prepotentials. The adrenaline-induced hyperpolarization may be due to an increase in K permeability but not to stimulation of electrogenic Na pumping. The increase in K permeability may depend upon the presence of Cl ions.", "contents": "The electrophysiological basis of the motor inhibitory effect of adrenaline on rabbit small intestinal smooth muscle. The spontaneous electrical activity of small intestinal smooth muscle cells consists of repetive depolarizations (control activity) on which spikes (response activity) may be superimposed; each spike is preceded by a prepotential. Response activity is associated with contractions. Adrenaline initially abolished the response activity and any prepotenials, as well as the contractions, without altering the control activity or the membrane potential. This effect was followed by hyperpolarization and slight increase in the control potential amplitude. The hyperpolarization was insenstitive tto temperature (Q10 = 1.01) and was larger when the membrane was initially depolarized by K withdrawal but did not occur after the membrane was hyperpolarized by replacing C1 by propionate or by prolonged K withdrawal. It is suggested that adrenaline inhibits intestinal motility by uncoupling the control activity to response activity through suppression of the prepotentials. The adrenaline-induced hyperpolarization may be due to an increase in K permeability but not to stimulation of electrogenic Na pumping. The increase in K permeability may depend upon the presence of Cl ions.", "PMID": 974873}
{"id": "pubmed23n0032_10012", "title": "The effects of certain drugs on the uptake and release of [3H]noradrenaline in rat whole brain homogenates.", "content": "Twelve drugs were studied with respect to their effects on inhbition of neuronal uptake of [3H]noradrenaline ([3H]NA) and on release of this amine from presynaptic nerve terminals. An in vitro method, using a crude synaptosomal homogenate prepared from rat whole brain, was employed. All drugs tested were found to produce some release of [3H]NA although tyramine was by far the most potent drug in this respect; tripelennamine and cocaine were observed to produce the least release. Studies of inhibition of NA uptake again demonstrated tyramine to be the most potent of the 12 drugs although in this case it did not differ significantly from cocaine and tripelennaine. The remaining compounds also showed decreased accumulation of [3H]NA and all 12 drugs produced uptake inhibition at a lower dose than that required for release of the amine. A correlation between releasing potency and lipophilicity of the compounds indicated that tyramine seemed to be acting in a different manner from the remaining compounds. A correlation between inhibitory potency and lipophilicity could be demonstrated for only six of the drugs, with tyramine, tripelennamine and cocaine showing the greatest deviation from this relationship.", "contents": "The effects of certain drugs on the uptake and release of [3H]noradrenaline in rat whole brain homogenates. Twelve drugs were studied with respect to their effects on inhbition of neuronal uptake of [3H]noradrenaline ([3H]NA) and on release of this amine from presynaptic nerve terminals. An in vitro method, using a crude synaptosomal homogenate prepared from rat whole brain, was employed. All drugs tested were found to produce some release of [3H]NA although tyramine was by far the most potent drug in this respect; tripelennamine and cocaine were observed to produce the least release. Studies of inhibition of NA uptake again demonstrated tyramine to be the most potent of the 12 drugs although in this case it did not differ significantly from cocaine and tripelennaine. The remaining compounds also showed decreased accumulation of [3H]NA and all 12 drugs produced uptake inhibition at a lower dose than that required for release of the amine. A correlation between releasing potency and lipophilicity of the compounds indicated that tyramine seemed to be acting in a different manner from the remaining compounds. A correlation between inhibitory potency and lipophilicity could be demonstrated for only six of the drugs, with tyramine, tripelennamine and cocaine showing the greatest deviation from this relationship.", "PMID": 974874}
{"id": "pubmed23n0032_10013", "title": "Lack of effect of alcohol on small intestinal binding of the vitamin B12 - intrinsic factor complex.", "content": "Absorption of 57Co-labelled vitamin B12 - intrinsic factor (IF) complex and its binding to mucosal precipitate and brush border fractions of rat small intestine was studied in rats pair-fed with a liquid diet containing ethanol 5 g/100 ml, 35% of calories, or isocalorically substituted sucrose. IF was obtained from rats fasted for 18 h. and for each experiment the amount of vitamin B12 added was the minimum required to achieve maximum binding to IF. Rats fed alcohol exhibited hepatic steatosis, proliferation of smooth endoplasmic reticulum, and disordered mitochondria after 6 weeks on the diet, and absorption of vitamin B12, fed with IF by stomach tube, was reduced signficantly. In contrast, binding of 57Co-labelled vitamin B12 -IF complex to mucosal precipitate and brush border fractions was never less than that of fractions from control rats at 4, 8 and 12 weeks on the alcohol diet. Furthermore, binding to the brush border was significantly greater in alcohol-fed rats at 12 weeks whether expressed per unit of beta-naphthylamidase (EC 3.4.1.1) activity or per milligram of protein. Total mucosal sucrase (EC 5.2.1.26) and beta-naphthylamidase were unchanged or slightly increased (beta-naphthylamidase at 12 weeks) on the alcohol-containing diet indicating that total brush border membrane was not reduced. Total brush border binding activity was the same in alcohol-fed and control rats at each time period. These results indicate that malabsorption of vitamin B12 in rats fed alcohol cannot be due to decreased binding of the vitamin B12 - IF complex by brush border membrane receptors, or secondary to a net decrease in membrane receptors.", "contents": "Lack of effect of alcohol on small intestinal binding of the vitamin B12 - intrinsic factor complex. Absorption of 57Co-labelled vitamin B12 - intrinsic factor (IF) complex and its binding to mucosal precipitate and brush border fractions of rat small intestine was studied in rats pair-fed with a liquid diet containing ethanol 5 g/100 ml, 35% of calories, or isocalorically substituted sucrose. IF was obtained from rats fasted for 18 h. and for each experiment the amount of vitamin B12 added was the minimum required to achieve maximum binding to IF. Rats fed alcohol exhibited hepatic steatosis, proliferation of smooth endoplasmic reticulum, and disordered mitochondria after 6 weeks on the diet, and absorption of vitamin B12, fed with IF by stomach tube, was reduced signficantly. In contrast, binding of 57Co-labelled vitamin B12 -IF complex to mucosal precipitate and brush border fractions was never less than that of fractions from control rats at 4, 8 and 12 weeks on the alcohol diet. Furthermore, binding to the brush border was significantly greater in alcohol-fed rats at 12 weeks whether expressed per unit of beta-naphthylamidase (EC 3.4.1.1) activity or per milligram of protein. Total mucosal sucrase (EC 5.2.1.26) and beta-naphthylamidase were unchanged or slightly increased (beta-naphthylamidase at 12 weeks) on the alcohol-containing diet indicating that total brush border membrane was not reduced. Total brush border binding activity was the same in alcohol-fed and control rats at each time period. These results indicate that malabsorption of vitamin B12 in rats fed alcohol cannot be due to decreased binding of the vitamin B12 - IF complex by brush border membrane receptors, or secondary to a net decrease in membrane receptors.", "PMID": 974875}
{"id": "pubmed23n0032_10014", "title": "Release of red cells from the slowly-exchanging splenic pool after noradrenaline administration.", "content": "Isolated, denervated, cat spleens were perfused at constant flow with modified Ringer solution. Perfusion, pressure, outflow rate, and outflow red cell concentration were measured against time. After splenic perfusion by 500 ml solution the cell washout curve became a single exponential function, indicating that only cells from the most slowly exchanging red cell compartment remained (these are immature and abnormal cells which adhere to the fine structures of the red pulp). Splenic contraction was induced by injection of 5 mug noradrenaline into the inflow after perfusion by 600 and 1000 ml of fluid, respectively; outflow cell concentration rose 17-fold before returning to baseline value and 32% of red cells in the spleen were expelled. The time course of changes in cell concentration was similar in shape but delayed with respect to that of outflow rate. The transit time of the cells from the site of release to the splenic vein must have exceeded 40 s, which is consistent only with release from the red pulp. Furthermore, at the peak of the cell concentration curve the mean reticulocyte count was 37.8%. Thus immature and abnormal red cells, which comprise the slowly-exchanging compartment, are indeed released from the spleen during contraction.", "contents": "Release of red cells from the slowly-exchanging splenic pool after noradrenaline administration. Isolated, denervated, cat spleens were perfused at constant flow with modified Ringer solution. Perfusion, pressure, outflow rate, and outflow red cell concentration were measured against time. After splenic perfusion by 500 ml solution the cell washout curve became a single exponential function, indicating that only cells from the most slowly exchanging red cell compartment remained (these are immature and abnormal cells which adhere to the fine structures of the red pulp). Splenic contraction was induced by injection of 5 mug noradrenaline into the inflow after perfusion by 600 and 1000 ml of fluid, respectively; outflow cell concentration rose 17-fold before returning to baseline value and 32% of red cells in the spleen were expelled. The time course of changes in cell concentration was similar in shape but delayed with respect to that of outflow rate. The transit time of the cells from the site of release to the splenic vein must have exceeded 40 s, which is consistent only with release from the red pulp. Furthermore, at the peak of the cell concentration curve the mean reticulocyte count was 37.8%. Thus immature and abnormal red cells, which comprise the slowly-exchanging compartment, are indeed released from the spleen during contraction.", "PMID": 974876}
{"id": "pubmed23n0032_10015", "title": "Action potential fatigue in nonmyelinated nerve fibers: garfish olfactory and rabbit vagus nerve.", "content": "A decrease in amplitude and conduction speed in the compound action potential is observed with time in gar olfactory and rabbit vagus nerve when it is stimulated between 4 and 15 Hz at 26 degrees C in vitro. The amplitude decays exponentially for 1-3 min before reaching a steady state. Recs 15 s for gar olfactory nerve and 50 s for rabbit vagus nerve. The steady state values are 14% and 36% of the original amplitude, respectively, and conduction speeds are reduced by 25 % in both nerves. The effect results from completion between ion flow during the action potential and active transport. The accumulation of K+ions and depletion of Na+ ions in the restricted extracellular space contributes to the amplitude fatigue while the depletion of Na+ ions alone causes the decrease in conduction speed. Ouabain increases the fatigue rate in both preparations, but more so for gar. The fatigue and recovery measurements may provide a useful method to investigate active pumping, including both the total pumping rate and the electrogenic component.", "contents": "Action potential fatigue in nonmyelinated nerve fibers: garfish olfactory and rabbit vagus nerve. A decrease in amplitude and conduction speed in the compound action potential is observed with time in gar olfactory and rabbit vagus nerve when it is stimulated between 4 and 15 Hz at 26 degrees C in vitro. The amplitude decays exponentially for 1-3 min before reaching a steady state. Recs 15 s for gar olfactory nerve and 50 s for rabbit vagus nerve. The steady state values are 14% and 36% of the original amplitude, respectively, and conduction speeds are reduced by 25 % in both nerves. The effect results from completion between ion flow during the action potential and active transport. The accumulation of K+ions and depletion of Na+ ions in the restricted extracellular space contributes to the amplitude fatigue while the depletion of Na+ ions alone causes the decrease in conduction speed. Ouabain increases the fatigue rate in both preparations, but more so for gar. The fatigue and recovery measurements may provide a useful method to investigate active pumping, including both the total pumping rate and the electrogenic component.", "PMID": 974877}
{"id": "pubmed23n0032_10016", "title": "Effects of viloxazine, an antidepressant agent, on biogenic amine uptake mechanisms and related activities.", "content": "The effects of viloxazine, a clinically effective antidepressant, on noradrenaline (NA) and 5-hydroxytryptamine (5-HT) uptake and various related pharmacological activities were determined and compared to those of the tricyclic antidepressants desimipramine, imipramine, and amitriptyline. Viloxazine inhibitied [3H]NA uptake in the mouse and rat heart, being maximally about one half as potent as imipramine with a similar onset, but shorter duration of action than imipramine. The drug did not inhibit [3H]NA uptake in rat medulla or hypothalamus in contrast to desimipramine and imipramine, but it did alter [3H]NA metabolites in a similar manner. Viloxazine, like desimipramine, was a weak blocker of mouse brain 5-HT uptake, but differed from desimipramine as it poteniated 5-HT-mediated functions in the mouse and rat, as did imipramine and amitriptyline, the latter drugs being relatively potent blockers of 5-HT uptake. Viloxazine potentiated the L-DOPA behavioural syndrome in the mouse, antagonized reserpine-induced ptosis and hypothermia in the mouse, and inhibited gastric acid secretion in the rat, but was less potent than the tricyclic antidepressants. No appreciable in vivo inhibition of monoamine oxidase (EC 1.4.3.4.) activity in the mouse was exhibited. Like imipramine, the drug potentiated the ocular effects of L-adrenaline in the rabbit. It was similar to imipramine in potency in potentiating the apomorphine-induced gnawing in the mouse. The drug antagonized oxotremorine-induced hypothermia in the mouse but differed from the tricyclic antidepressants in not exhibiting the anticholinergic effects of blocking the tremors, salivation and lacrimation. Thus, viloxazine exhibits activities related to the biogenic amines both similar to and different from the tricyclics desimipramine, imipramine, and amitriptyline. These actions appear to be of relevance with respect to the antidepressant action of this drug.", "contents": "Effects of viloxazine, an antidepressant agent, on biogenic amine uptake mechanisms and related activities. The effects of viloxazine, a clinically effective antidepressant, on noradrenaline (NA) and 5-hydroxytryptamine (5-HT) uptake and various related pharmacological activities were determined and compared to those of the tricyclic antidepressants desimipramine, imipramine, and amitriptyline. Viloxazine inhibitied [3H]NA uptake in the mouse and rat heart, being maximally about one half as potent as imipramine with a similar onset, but shorter duration of action than imipramine. The drug did not inhibit [3H]NA uptake in rat medulla or hypothalamus in contrast to desimipramine and imipramine, but it did alter [3H]NA metabolites in a similar manner. Viloxazine, like desimipramine, was a weak blocker of mouse brain 5-HT uptake, but differed from desimipramine as it poteniated 5-HT-mediated functions in the mouse and rat, as did imipramine and amitriptyline, the latter drugs being relatively potent blockers of 5-HT uptake. Viloxazine potentiated the L-DOPA behavioural syndrome in the mouse, antagonized reserpine-induced ptosis and hypothermia in the mouse, and inhibited gastric acid secretion in the rat, but was less potent than the tricyclic antidepressants. No appreciable in vivo inhibition of monoamine oxidase (EC 1.4.3.4.) activity in the mouse was exhibited. Like imipramine, the drug potentiated the ocular effects of L-adrenaline in the rabbit. It was similar to imipramine in potency in potentiating the apomorphine-induced gnawing in the mouse. The drug antagonized oxotremorine-induced hypothermia in the mouse but differed from the tricyclic antidepressants in not exhibiting the anticholinergic effects of blocking the tremors, salivation and lacrimation. Thus, viloxazine exhibits activities related to the biogenic amines both similar to and different from the tricyclics desimipramine, imipramine, and amitriptyline. These actions appear to be of relevance with respect to the antidepressant action of this drug.", "PMID": 974878}
{"id": "pubmed23n0032_10017", "title": "The effect of taurine on calcium exchange of sarcoplasmic reticulum of guinea pig heart studied by means of dialysis kinetics.", "content": "Calcium binding and release by sarcoplasmic reticulum vesicles of guinea pig heart were studied by means of a dialysis apparatus and analyzed with a model of three closed compartments. The rate constants of calcium binding were increased by taurine, and this effect was dose-related, whereas calcium efflux rate constants were very near to 0 in control experiments and were not effected by taurine. Taurine therefore increased the calcium content of sarcoplasmic vesicles and decreased the calcium concentration of the medium. beta-Alanine had no action on calcium fluxes.", "contents": "The effect of taurine on calcium exchange of sarcoplasmic reticulum of guinea pig heart studied by means of dialysis kinetics. Calcium binding and release by sarcoplasmic reticulum vesicles of guinea pig heart were studied by means of a dialysis apparatus and analyzed with a model of three closed compartments. The rate constants of calcium binding were increased by taurine, and this effect was dose-related, whereas calcium efflux rate constants were very near to 0 in control experiments and were not effected by taurine. Taurine therefore increased the calcium content of sarcoplasmic vesicles and decreased the calcium concentration of the medium. beta-Alanine had no action on calcium fluxes.", "PMID": 974879}
{"id": "pubmed23n0032_10018", "title": "A dual mechanism for the anticonvulsant action of aminooxyacetic acid.", "content": "The intramuscular injection of aminooxyacetic acid (AOAA) into mice elevated the concentration of gamma-aminobutyric acid (GABA) in the brain, inhibited glutamic acid decarboxylase activity and delayed the onset of isonicotinic acid hydrazide induced seizures. Analyses of these results and of those obtained previously by the authors and other workers indicated that the anticonvulsant action of AOAA involved two mechanisms. One, involving GABA metabolism, was most effective 6 h after AOAA administration, and the other, not involving GABA, was maximally effective 1.5 h after AOAA injection and was completely absent after 6 h. Depending on the convulsant agent under study, the mechanism of the anticonvulsant action of AOAA was purely of the GABA type, purely of the non-GABA type or a combination of both types.", "contents": "A dual mechanism for the anticonvulsant action of aminooxyacetic acid. The intramuscular injection of aminooxyacetic acid (AOAA) into mice elevated the concentration of gamma-aminobutyric acid (GABA) in the brain, inhibited glutamic acid decarboxylase activity and delayed the onset of isonicotinic acid hydrazide induced seizures. Analyses of these results and of those obtained previously by the authors and other workers indicated that the anticonvulsant action of AOAA involved two mechanisms. One, involving GABA metabolism, was most effective 6 h after AOAA administration, and the other, not involving GABA, was maximally effective 1.5 h after AOAA injection and was completely absent after 6 h. Depending on the convulsant agent under study, the mechanism of the anticonvulsant action of AOAA was purely of the GABA type, purely of the non-GABA type or a combination of both types.", "PMID": 974880}
{"id": "pubmed23n0032_10019", "title": "Toxicity testing in vitro. I. The effects of delta 9-tetrahydrocannabinol and aflatoxin B1 on the growth of cultured human fibroblasts.", "content": "The acute toxicity of delta 9-tetrahydrocannabinol (delta 9-THC) and aflatoxin B1 to two strains of cultured human fibroblasts has been studied. delta 9-THC had no effect on cell plating efficiency or on the growth of mass cultures at doses of 1 mug/ml (3.18 muM) or less; at 10 mug/ml plating efficiency was reduced by approximately half and at 20 mug/ml colony formation was zero. Aflatoxin B1 reduced plating efficiency at dose levels of 0.1 mug/ml (0.32 muM) and above; in mass clultures it retarded growth at 1 mug/ml and produced complete inhibition at 5 mug/ml. The potential usefulness of cultured human fibroblasts in toxicity testing is discussed and the importance of using normal diploid cells rather than aneuploid permanent lines is emphasized. The limitations of cell cultures in assessing toxicity, and possible solutions to these are considered.", "contents": "Toxicity testing in vitro. I. The effects of delta 9-tetrahydrocannabinol and aflatoxin B1 on the growth of cultured human fibroblasts. The acute toxicity of delta 9-tetrahydrocannabinol (delta 9-THC) and aflatoxin B1 to two strains of cultured human fibroblasts has been studied. delta 9-THC had no effect on cell plating efficiency or on the growth of mass cultures at doses of 1 mug/ml (3.18 muM) or less; at 10 mug/ml plating efficiency was reduced by approximately half and at 20 mug/ml colony formation was zero. Aflatoxin B1 reduced plating efficiency at dose levels of 0.1 mug/ml (0.32 muM) and above; in mass clultures it retarded growth at 1 mug/ml and produced complete inhibition at 5 mug/ml. The potential usefulness of cultured human fibroblasts in toxicity testing is discussed and the importance of using normal diploid cells rather than aneuploid permanent lines is emphasized. The limitations of cell cultures in assessing toxicity, and possible solutions to these are considered.", "PMID": 974881}
{"id": "pubmed23n0032_10020", "title": "Toxicity testing in vitro. II. Use of a microsome - cultured human fibroblast system to study the cytotoxicity of cyclophosphamide.", "content": "The toxicity of cyclophosphamide (CP), has been studied using a human fibroblast cell culture system. A method is described in which CP is incubated with liver microsomes and appropriate co-factors before addition to the cells. This permits activation of the CP to its cytotoxic metabolite but avoids the necessity of adding microsomes directly to the cell culture. It was demonstrated that under these conditions CP was irreversibly toxic to the cells within hours at dose levels larger than or equal to 0.3 mg/ml. In the absence of microsomal activation it was toxic at similar dose levels but the toxic effects required several days to develop.", "contents": "Toxicity testing in vitro. II. Use of a microsome - cultured human fibroblast system to study the cytotoxicity of cyclophosphamide. The toxicity of cyclophosphamide (CP), has been studied using a human fibroblast cell culture system. A method is described in which CP is incubated with liver microsomes and appropriate co-factors before addition to the cells. This permits activation of the CP to its cytotoxic metabolite but avoids the necessity of adding microsomes directly to the cell culture. It was demonstrated that under these conditions CP was irreversibly toxic to the cells within hours at dose levels larger than or equal to 0.3 mg/ml. In the absence of microsomal activation it was toxic at similar dose levels but the toxic effects required several days to develop.", "PMID": 974882}
{"id": "pubmed23n0032_10021", "title": "Characterization of natriuretic activity from posterior pituitary lobes.", "content": "Previous studies have indicated the existence of natriuretic factors of hormonal nature with the posterior pituitary gland as a possible site of origin. It was in this light that a series of experiments was designed to examine the posterior pituitary for such factors. Acetic acid extracts of porcine and bovine posterior pituitary lobe tissue were subjected to gel filtration on Sephadex G-25. Several fractions in the molecular size range of 1000 were obtained which possessed potent natriuretic activity as assayed in rats. The activity of these fractions maximally increased sodium excretion to 6-8 muequiv./min, a 10- to 40-fold increase above control, when administered intraperitoneally to hydropenic, conscious rats. However, oxytocin and vasopressin, present in the posterior pituitary are natriuretic. These hormones were measured by radioimmunoassay, and invariably only those fractions which contained vasopressin and (or) oxytocin possessed natriuretic activity. Moreover, the extent of the natriuresis could be accounted for by the vasopressin and (or) oxytocin content of the test fractions. The natriuretic property of this material was abolished by treatment with thioglycollate. Further purification of natriuretic fractions by ion exchange resins, thin-layer chromatography and isoelectric focusing failed to resolve natriuretic activity from vasopressin and oxytocin. Similar results were observed following analysis of fractions isolated by gel filtration of acetic acid extracts of ventral hypothalamus tissue. The natriuretic fractions isolated from hypothalamic tissue were indistinguishable from oxytocin and vasopressin. These experiments suggest that the natriuretic activity in neurohypophyseal extracts can be attributed to oxytocin and vasopressin.", "contents": "Characterization of natriuretic activity from posterior pituitary lobes. Previous studies have indicated the existence of natriuretic factors of hormonal nature with the posterior pituitary gland as a possible site of origin. It was in this light that a series of experiments was designed to examine the posterior pituitary for such factors. Acetic acid extracts of porcine and bovine posterior pituitary lobe tissue were subjected to gel filtration on Sephadex G-25. Several fractions in the molecular size range of 1000 were obtained which possessed potent natriuretic activity as assayed in rats. The activity of these fractions maximally increased sodium excretion to 6-8 muequiv./min, a 10- to 40-fold increase above control, when administered intraperitoneally to hydropenic, conscious rats. However, oxytocin and vasopressin, present in the posterior pituitary are natriuretic. These hormones were measured by radioimmunoassay, and invariably only those fractions which contained vasopressin and (or) oxytocin possessed natriuretic activity. Moreover, the extent of the natriuresis could be accounted for by the vasopressin and (or) oxytocin content of the test fractions. The natriuretic property of this material was abolished by treatment with thioglycollate. Further purification of natriuretic fractions by ion exchange resins, thin-layer chromatography and isoelectric focusing failed to resolve natriuretic activity from vasopressin and oxytocin. Similar results were observed following analysis of fractions isolated by gel filtration of acetic acid extracts of ventral hypothalamus tissue. The natriuretic fractions isolated from hypothalamic tissue were indistinguishable from oxytocin and vasopressin. These experiments suggest that the natriuretic activity in neurohypophyseal extracts can be attributed to oxytocin and vasopressin.", "PMID": 974883}
{"id": "pubmed23n0032_10022", "title": "Nonparticipation of adrenergic mechanism in potassium-induced relaxation of taenia from guinea pig caecum.", "content": "In the isolated taenia caeci of guinea pigs excess potassium (10-30 mM) induced a phasic relaxation followed by a contraction. This phasic relaxation was unaffected by treatment with  hexamethonium, phentolamine, propranolol and bretylium. However, relaxation induced by perivascular nerve stimulation was inhibited by all these agents but not by hexamethonium. Tetrodotoxin inhibited both forms of relaxation. Potassium-induced relaxation was not accompanied by [3H]noradrenaline release. Perivascular nerve stimulation caused release of [3H]noradrenaline and this was blocked by bretylium. Treatment with ouabain or replacement of NaCl by LiCl, but not treatment by cold storage, inhibited the potassium-induced relaxation. These results suggest that the potassium-induced relaxation of taenia caeci was due to electrogenic sodium pumping and was independent of the adrenergic innervation of the tissue.", "contents": "Nonparticipation of adrenergic mechanism in potassium-induced relaxation of taenia from guinea pig caecum. In the isolated taenia caeci of guinea pigs excess potassium (10-30 mM) induced a phasic relaxation followed by a contraction. This phasic relaxation was unaffected by treatment with  hexamethonium, phentolamine, propranolol and bretylium. However, relaxation induced by perivascular nerve stimulation was inhibited by all these agents but not by hexamethonium. Tetrodotoxin inhibited both forms of relaxation. Potassium-induced relaxation was not accompanied by [3H]noradrenaline release. Perivascular nerve stimulation caused release of [3H]noradrenaline and this was blocked by bretylium. Treatment with ouabain or replacement of NaCl by LiCl, but not treatment by cold storage, inhibited the potassium-induced relaxation. These results suggest that the potassium-induced relaxation of taenia caeci was due to electrogenic sodium pumping and was independent of the adrenergic innervation of the tissue.", "PMID": 974884}
{"id": "pubmed23n0032_10023", "title": "The thyroidal response to chronic goitrogenic stimulation and the persistence of effects of early goitrogenic stimulation.", "content": "The effects of overstimulation of the thyroid gland induced by the feeding or rapeseed meal or thiouracil during the growing period persisted for as long as 75 weeks after withdrawal of the source of goitrogen from the diet. Thyroid weight, thyroid iodine content and radioiodine uptake were all increased in birds which had received goitrogen during the growing period. Follicle diameter was greater and the height of epithelial cells was lower than in control birds. The thyroid changes in these birds were deduced to be compensatory because production characteristics were normal and distribution of a dose of radioiodine into thyronines (T3 + T4) was similar in the birds which had received goitrogen during the growing period and in the control birds. Birds which were being fed rapeseed meal at the time the thyroid gland were examined showed effects which varied in magnitude according to the length of time over which rapeseed meal had been fed. Thyroid weight, follicle diameter, amount of epithelial tissue present and the total amount of iodine in the thyroid glands increased, whereas coupling efficiency (radioiodine-labelled T3 + T4)/(monoiodotyrosine/diiodotyrosine) declined with time.", "contents": "The thyroidal response to chronic goitrogenic stimulation and the persistence of effects of early goitrogenic stimulation. The effects of overstimulation of the thyroid gland induced by the feeding or rapeseed meal or thiouracil during the growing period persisted for as long as 75 weeks after withdrawal of the source of goitrogen from the diet. Thyroid weight, thyroid iodine content and radioiodine uptake were all increased in birds which had received goitrogen during the growing period. Follicle diameter was greater and the height of epithelial cells was lower than in control birds. The thyroid changes in these birds were deduced to be compensatory because production characteristics were normal and distribution of a dose of radioiodine into thyronines (T3 + T4) was similar in the birds which had received goitrogen during the growing period and in the control birds. Birds which were being fed rapeseed meal at the time the thyroid gland were examined showed effects which varied in magnitude according to the length of time over which rapeseed meal had been fed. Thyroid weight, follicle diameter, amount of epithelial tissue present and the total amount of iodine in the thyroid glands increased, whereas coupling efficiency (radioiodine-labelled T3 + T4)/(monoiodotyrosine/diiodotyrosine) declined with time.", "PMID": 974885}
{"id": "pubmed23n0032_10024", "title": "Pituitary response to auditory stress: effect of treatment with alpha-methyl-p-tyrosine. Usefulness of a factorial mixed design for statistical analysis.", "content": "Eight adult male rats were chronically cannulated in the jugular vein and placed individually in a sound-attenuated cubicle. Four of the animals were also implanted with a permanent cannula in the right lateral ventricle of the brain. Each animal was submitted twice to auditory stress at a 24-h interval. Before each stress, the rats were pretreated with either saline or alpha-methyl-p-tyrosine (alpha-MT), the order of administration of the drug and its vehicle being alternated in the eight rats. The injections were made either intravenously or intraventricularly. Auditory stress significantly depressed plasma growth hormone (GH) levels irrespective of the type of pretreatment. Mean plasma GH levels were significantly lower after alpha-MT pretreatment. alpha-MT pretreated animals had higher mean plasma corticosterone (B) levels which remained unchanged during stress. Plasma follicle stimulating hormone (FSH) levels were not modified by stress nor by alpha-MT pretreatment. The intraventricular administration of alpha-MT at a dose (20 mg/kg) which is ineffective by a systemic route produced the same effects on GH and B levels as the intravenous injection (250 mg/kg). These data seem to indicate that auditory stress exerts its inhibitory effect on GH secretion through a noncatecholaminergic pathway. They show, on the other hand, the existence of a central catecholaminergic tonus, stimulatory for GH and inhibitory for B. Statistical analysis was based on a factorial mixed design for repeated measurements after logarithmic transformation of the data. The purpose, advantages and limits of this procedure are presented and discussed.", "contents": "Pituitary response to auditory stress: effect of treatment with alpha-methyl-p-tyrosine. Usefulness of a factorial mixed design for statistical analysis. Eight adult male rats were chronically cannulated in the jugular vein and placed individually in a sound-attenuated cubicle. Four of the animals were also implanted with a permanent cannula in the right lateral ventricle of the brain. Each animal was submitted twice to auditory stress at a 24-h interval. Before each stress, the rats were pretreated with either saline or alpha-methyl-p-tyrosine (alpha-MT), the order of administration of the drug and its vehicle being alternated in the eight rats. The injections were made either intravenously or intraventricularly. Auditory stress significantly depressed plasma growth hormone (GH) levels irrespective of the type of pretreatment. Mean plasma GH levels were significantly lower after alpha-MT pretreatment. alpha-MT pretreated animals had higher mean plasma corticosterone (B) levels which remained unchanged during stress. Plasma follicle stimulating hormone (FSH) levels were not modified by stress nor by alpha-MT pretreatment. The intraventricular administration of alpha-MT at a dose (20 mg/kg) which is ineffective by a systemic route produced the same effects on GH and B levels as the intravenous injection (250 mg/kg). These data seem to indicate that auditory stress exerts its inhibitory effect on GH secretion through a noncatecholaminergic pathway. They show, on the other hand, the existence of a central catecholaminergic tonus, stimulatory for GH and inhibitory for B. Statistical analysis was based on a factorial mixed design for repeated measurements after logarithmic transformation of the data. The purpose, advantages and limits of this procedure are presented and discussed.", "PMID": 974886}
{"id": "pubmed23n0032_10025", "title": "The effects of oral diazepam pretreatment on the biliary excretion of sulfobromophthalein in rats.", "content": "Studies were performed with rats to examine the effects of single, as well as repetitive oral diazepam (DZP) pretreatment on biliary sulfobromophthalein (BSP) excretion rates and on bile flow parameters. One-hour pretreatment of male rats with 150 mg/kg of DZP resulted in about a one-third reduction in the peak biliary excretion rate of BSP (60 mg/kg, iv) and this was associated with a decrease in relative proportions of conjugated BSP in bile. The biliary excretion of preconjugated BSP was unaffected. BSP hepatic uptake and storage were apparently unaffected. In vitro DZP markedly inhibited BSP conjugating activity. In contrast to the above results, when BSP excretion was examined 1 h after the last of five daily oral doses of DZP (150 mg kg-1 day-1), no change in the peak elimination rate of this dye was evident. However, bile flow rates were higher in DZP-treated rats than in controls. When rats were examined 24 h after the last of five daily oral doses of DZP (150 mg/kg), the choleretic response persisted. Further studies showed that the repetitive DZP pretreatment enhanced the bile salt-independent mechanisms of bile formation.", "contents": "The effects of oral diazepam pretreatment on the biliary excretion of sulfobromophthalein in rats. Studies were performed with rats to examine the effects of single, as well as repetitive oral diazepam (DZP) pretreatment on biliary sulfobromophthalein (BSP) excretion rates and on bile flow parameters. One-hour pretreatment of male rats with 150 mg/kg of DZP resulted in about a one-third reduction in the peak biliary excretion rate of BSP (60 mg/kg, iv) and this was associated with a decrease in relative proportions of conjugated BSP in bile. The biliary excretion of preconjugated BSP was unaffected. BSP hepatic uptake and storage were apparently unaffected. In vitro DZP markedly inhibited BSP conjugating activity. In contrast to the above results, when BSP excretion was examined 1 h after the last of five daily oral doses of DZP (150 mg kg-1 day-1), no change in the peak elimination rate of this dye was evident. However, bile flow rates were higher in DZP-treated rats than in controls. When rats were examined 24 h after the last of five daily oral doses of DZP (150 mg/kg), the choleretic response persisted. Further studies showed that the repetitive DZP pretreatment enhanced the bile salt-independent mechanisms of bile formation.", "PMID": 974887}
{"id": "pubmed23n0032_10026", "title": "Inotropic and chronotropic responses of the in vivo denervated dog myocardium to dobutamine.", "content": "The inotropic and chronotropic responses to dobutamine (DBA) and isoprenaline (5ISO) were examined in eight chloralose anaesthetised dogs. Following acute cardiac denervation, heart rate (HR) and contractility (dP/dtmax), measured at a fixed paced atrial rate, were recorded during intravenous infusion of incremental doses of DBA and ISO. Both DBA and ISO elicited increases in HR and dP/dtmax. The increases in dP/dtmax for a one beat per minute increase in HR was 102.0 +/- 10.6 mm Hg/s (1 mm Hg (0 degree C) = 133.322 Pa), during DBA infusion, and 61.5 +/- 8.4 mm Hg/s during ISO infusion. It appeared that the relatively greater inotropic effect of DBA in comparison with ISO was the result of an augmentation of its inotropic activity. DBA infusion was accompanied by a significant increase in mean aortic pressure at all doses examined. An increase in afterload may account for part of the increased inotropic responses to DBA.", "contents": "Inotropic and chronotropic responses of the in vivo denervated dog myocardium to dobutamine. The inotropic and chronotropic responses to dobutamine (DBA) and isoprenaline (5ISO) were examined in eight chloralose anaesthetised dogs. Following acute cardiac denervation, heart rate (HR) and contractility (dP/dtmax), measured at a fixed paced atrial rate, were recorded during intravenous infusion of incremental doses of DBA and ISO. Both DBA and ISO elicited increases in HR and dP/dtmax. The increases in dP/dtmax for a one beat per minute increase in HR was 102.0 +/- 10.6 mm Hg/s (1 mm Hg (0 degree C) = 133.322 Pa), during DBA infusion, and 61.5 +/- 8.4 mm Hg/s during ISO infusion. It appeared that the relatively greater inotropic effect of DBA in comparison with ISO was the result of an augmentation of its inotropic activity. DBA infusion was accompanied by a significant increase in mean aortic pressure at all doses examined. An increase in afterload may account for part of the increased inotropic responses to DBA.", "PMID": 974888}
{"id": "pubmed23n0032_10027", "title": "Interaction of propranolol and phentolamine with ethanol in the rat.", "content": "The possible role of the adrenergic nervous system in the intoxicant effects of ethanol was examined in studies of the interaction or propranolol and phentolamine with ethanol. Propranolol teneded to increase the effect of lower doses of ethanol in a dose-dependent manner. However, the effect of higher doses of ethanol (over 2.0 g/kg) tended to be diminished by low doses of propranolol, whereas higher doses of propranolol were ineffective or actually increased the ethanol effect. Phentolamine tended to decrease the effect of the lower ethanol doses. These findings are inconsistent with any simple adrenergic mechanism in the mediation of the intoxicant effect of ethanol.", "contents": "Interaction of propranolol and phentolamine with ethanol in the rat. The possible role of the adrenergic nervous system in the intoxicant effects of ethanol was examined in studies of the interaction or propranolol and phentolamine with ethanol. Propranolol teneded to increase the effect of lower doses of ethanol in a dose-dependent manner. However, the effect of higher doses of ethanol (over 2.0 g/kg) tended to be diminished by low doses of propranolol, whereas higher doses of propranolol were ineffective or actually increased the ethanol effect. Phentolamine tended to decrease the effect of the lower ethanol doses. These findings are inconsistent with any simple adrenergic mechanism in the mediation of the intoxicant effect of ethanol.", "PMID": 974889}
{"id": "pubmed23n0032_10028", "title": "The relaxant effect of adenosine-5'-alpha, beta-methylenediphosphonate on the longitudinal smooth muscle of the rabbit ileum.", "content": "The response pattern of the isolated longitudinal muscle of the rabbit ileum to adenosine-5'-alpha, beta-methylenediphosphonate (APCP) was found to differ from that caused by adenosine or ATP in that the rapid initial phase of relaxation is absent and that at doses above 10 muM a secondary increase of contractile activity occurs in the continued presence of the nucleotide. This secondary phase was attenuated after indomethacin pretreatment and reestablishement of tone with acetylcholine. Theophylline did not modify APCP-induced relaxations while effectively antagonizing those caused by adenosine. It appears that APCP acts via a receptor site or pathway distinct from that of adenosine or ATP.", "contents": "The relaxant effect of adenosine-5'-alpha, beta-methylenediphosphonate on the longitudinal smooth muscle of the rabbit ileum. The response pattern of the isolated longitudinal muscle of the rabbit ileum to adenosine-5'-alpha, beta-methylenediphosphonate (APCP) was found to differ from that caused by adenosine or ATP in that the rapid initial phase of relaxation is absent and that at doses above 10 muM a secondary increase of contractile activity occurs in the continued presence of the nucleotide. This secondary phase was attenuated after indomethacin pretreatment and reestablishement of tone with acetylcholine. Theophylline did not modify APCP-induced relaxations while effectively antagonizing those caused by adenosine. It appears that APCP acts via a receptor site or pathway distinct from that of adenosine or ATP.", "PMID": 974890}
{"id": "pubmed23n0032_10029", "title": "Differing estrogenic activities for the enantiomers of o, p'-DDT in immature female rats.", "content": "Female rats (20-21 days) were given single intraperitoneal injections of (+/-)-o,p'-DDT, (--)-o,p'-DDT, or (+)-o,p'-DDT. At 18 h their uteri were excised and the estrogen sensitive parameters, uterine wet weight, and uterine glycogen content were measured. For o,p'-DDT, the levo enantiomer is the more active estrogen in immature female rats. Optical resolutions of other racemic environmental xenobiotics may be important in the evaluation of their biological effects.", "contents": "Differing estrogenic activities for the enantiomers of o, p'-DDT in immature female rats. Female rats (20-21 days) were given single intraperitoneal injections of (+/-)-o,p'-DDT, (--)-o,p'-DDT, or (+)-o,p'-DDT. At 18 h their uteri were excised and the estrogen sensitive parameters, uterine wet weight, and uterine glycogen content were measured. For o,p'-DDT, the levo enantiomer is the more active estrogen in immature female rats. Optical resolutions of other racemic environmental xenobiotics may be important in the evaluation of their biological effects.", "PMID": 974891}
{"id": "pubmed23n0032_10030", "title": "Extracellular nuclease produced by a marine bacterium. I. Extracellular deoxyribonuclease formation by a marine Vibrio sp.", "content": "Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracellular DNase formation. The DNase activity could not be detected in the synthetic seawater culture liquid lacking magnesium ion, and DNase activity decreased in a calcium-deficient medium. The optimum temperature for the growth of this organism was between 15 and 25 degrees C. The formation of extracellular DNase was the greatest at 20 degrees C and less activity was found at 10 and 30 degrees C.", "contents": "Extracellular nuclease produced by a marine bacterium. I. Extracellular deoxyribonuclease formation by a marine Vibrio sp. Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracellular DNase formation. The DNase activity could not be detected in the synthetic seawater culture liquid lacking magnesium ion, and DNase activity decreased in a calcium-deficient medium. The optimum temperature for the growth of this organism was between 15 and 25 degrees C. The formation of extracellular DNase was the greatest at 20 degrees C and less activity was found at 10 and 30 degrees C.", "PMID": 974900}
{"id": "pubmed23n0032_10031", "title": "Macrophage function and host resistance against infection with Toxoplasma gondii.", "content": "The role of macrophages on the course of an infection with Toxoplasma gondii has been examined. Stimulation of macrophage function by killed Bordetella pertussis cells did not show any beneficial effect as an increased susceptibility became apparent. The functional blockade of macrophages by dextran sulfate or carbon particles did not result in a higher susceptibility of mice to the lethal primary infection with T. gondii. Thus in vivo macrophages apparently do not play an essential role as effector cells as they do in infections with other obligate intracellular infective organisms such as Listeria monocytogenes. The spleen is apparently of crucial importance for resistance against T. gondii infection, since death occurred earlier in splenectomized mice than in control animals.", "contents": "Macrophage function and host resistance against infection with Toxoplasma gondii. The role of macrophages on the course of an infection with Toxoplasma gondii has been examined. Stimulation of macrophage function by killed Bordetella pertussis cells did not show any beneficial effect as an increased susceptibility became apparent. The functional blockade of macrophages by dextran sulfate or carbon particles did not result in a higher susceptibility of mice to the lethal primary infection with T. gondii. Thus in vivo macrophages apparently do not play an essential role as effector cells as they do in infections with other obligate intracellular infective organisms such as Listeria monocytogenes. The spleen is apparently of crucial importance for resistance against T. gondii infection, since death occurred earlier in splenectomized mice than in control animals.", "PMID": 974901}
{"id": "pubmed23n0032_10032", "title": "Isolation and characterization of nocardia-like variants of Mycobacterium smegmatis.", "content": "Orange-red-pigmented (OR) colonies were isolated from cream-yellow-pigmented Mycobacterium smegmatis after exposure to either mycobacteriophage MC4 or ultraviolet light; these variant strains were designated OR4 and ORuv, respectively. Early subculture of OR-colonies did not show any segregation of parental-type cells. However, colonies resembling the parental strains, possibly representing a back mutant (REV-OR4), were occasionally found during subculture of established OR-colonies or upon treatment with N-nitrose-N'-nitro-N-methylguanidine. The OR-variants were characterized by their lytic response to nocardiophage, but not to mycobacteriophages, presence of alpha-branched, beta-hydroxylated fatty acids of the Nocardia-type, and a guanine plus cytosine value of deoxyribonucleic acid (DNA) between 62 and 64 mol%. They were more resistant to the lethal action of both ultraviolet light and mitomycin C treatment than the parental and back mutant strains. Although the OR-variants in this study possess characteristics common to the genus Nocardia or some of the 'rhodochrous' mycobacteria, evidence is presented that they form a new class of mycobacterial variants.", "contents": "Isolation and characterization of nocardia-like variants of Mycobacterium smegmatis. Orange-red-pigmented (OR) colonies were isolated from cream-yellow-pigmented Mycobacterium smegmatis after exposure to either mycobacteriophage MC4 or ultraviolet light; these variant strains were designated OR4 and ORuv, respectively. Early subculture of OR-colonies did not show any segregation of parental-type cells. However, colonies resembling the parental strains, possibly representing a back mutant (REV-OR4), were occasionally found during subculture of established OR-colonies or upon treatment with N-nitrose-N'-nitro-N-methylguanidine. The OR-variants were characterized by their lytic response to nocardiophage, but not to mycobacteriophages, presence of alpha-branched, beta-hydroxylated fatty acids of the Nocardia-type, and a guanine plus cytosine value of deoxyribonucleic acid (DNA) between 62 and 64 mol%. They were more resistant to the lethal action of both ultraviolet light and mitomycin C treatment than the parental and back mutant strains. Although the OR-variants in this study possess characteristics common to the genus Nocardia or some of the 'rhodochrous' mycobacteria, evidence is presented that they form a new class of mycobacterial variants.", "PMID": 974902}
{"id": "pubmed23n0032_10033", "title": "The effects of glucosamine derivatives on the growth of cultivable Treponema.", "content": "The effects of glucosamine and its derivatives on the growth of Treponema phagedenis biotypes reiter and kazan 5 were studied to determine if these agents could act as growth stimulants for organisms of the genus Treptonema. Growth studies on the cultivable treponemes were conducted in culture tubes containing glucosamine, glucosamine derivatives, and (or) glucose and galactose in serum-based media. Glucosamine and N-phenylacetylglucosamine were definitely inhibitory to growth at the concentrations studied. N-acetylglucosamine (NAG) produced mixed effects.", "contents": "The effects of glucosamine derivatives on the growth of cultivable Treponema. The effects of glucosamine and its derivatives on the growth of Treponema phagedenis biotypes reiter and kazan 5 were studied to determine if these agents could act as growth stimulants for organisms of the genus Treptonema. Growth studies on the cultivable treponemes were conducted in culture tubes containing glucosamine, glucosamine derivatives, and (or) glucose and galactose in serum-based media. Glucosamine and N-phenylacetylglucosamine were definitely inhibitory to growth at the concentrations studied. N-acetylglucosamine (NAG) produced mixed effects.", "PMID": 974903}
{"id": "pubmed23n0032_10034", "title": "Investigations of nitrogenase activity in rheotrophic peat.", "content": "Acetylene reduction by the peat microflora was unaffected by light, but was sensitive to temperature, with an optimum of about 30 degrees C. The nitrogenase was inactivated by exposure to temperatures above about 35 degrees C. Activity occurred in completely anaerobic conditions; the rate of ethylene production was of the order of 0.5 nmol C2H4 ml-1 h-1. Experiments with time courses indicated that exposure to oxygen caused an initial inhibition of activity followed by a period in which ethylene production was stimulated to rates much higher than in the anaerobic tests; both the inhibition and stimulation appeared to be related to the level of oxygenation. It is suggested that these results could be explained by the existence of nitrogen-fixing associations of aerobic and anaerobic bacteria in the peat. The stimulation of activity caused by exposure to air indicates that care must be taken when interpreting results of the assay obtained from waterlogged organic soils.", "contents": "Investigations of nitrogenase activity in rheotrophic peat. Acetylene reduction by the peat microflora was unaffected by light, but was sensitive to temperature, with an optimum of about 30 degrees C. The nitrogenase was inactivated by exposure to temperatures above about 35 degrees C. Activity occurred in completely anaerobic conditions; the rate of ethylene production was of the order of 0.5 nmol C2H4 ml-1 h-1. Experiments with time courses indicated that exposure to oxygen caused an initial inhibition of activity followed by a period in which ethylene production was stimulated to rates much higher than in the anaerobic tests; both the inhibition and stimulation appeared to be related to the level of oxygenation. It is suggested that these results could be explained by the existence of nitrogen-fixing associations of aerobic and anaerobic bacteria in the peat. The stimulation of activity caused by exposure to air indicates that care must be taken when interpreting results of the assay obtained from waterlogged organic soils.", "PMID": 974904}
{"id": "pubmed23n0032_10035", "title": "Effect of magnesium and some nutrients on the growth and nuclease formation of a moderate halophile, Micrococcus varians var. halophilus.", "content": "Production of halophilic nuclease by a moderate halophile, Micrococcus varians, ATCC 21971, was maximal at 2.5 to 3.5 M NaCl concentration in a complex medium (CM) composed of 1% casamino acids, 1% yeast extract, and NaCl. The addition of 81 mM MgSO4 to CM inhibited nuclease production in spite of good growth. Microscopic observation showed that this inhibition was accompanied by complete clumping of the cells. The Sehgal and Gibbons complex medium (SGC) which contained 0.75% vitamin-free casamino acids, 1% yeast extract, and NaCl, however, supported good production of the nuclease in spite of the presence of 81 mM MgSO4. It seemed that both magnesium sulfate and some substances present in CM might be responsible for this inhibition and clumping. A synthetic medium optimal for enzyme production was developed consisting of 16 amino acids, 4 vitamins, 0.73 mM KH2PO4, 2.7 mM KCl, 20 mM MgSO4, and 2.5 M NaCl. The organism required biotin as an essential growth factor, and thiamine, riboflavin, and choline as stimulating factors. Omission of isoleucine from the medium reduced markedly the growth rate. Glutamic acid, proline, and arginine were consumed completely during cultivation in the synthetic medium.", "contents": "Effect of magnesium and some nutrients on the growth and nuclease formation of a moderate halophile, Micrococcus varians var. halophilus. Production of halophilic nuclease by a moderate halophile, Micrococcus varians, ATCC 21971, was maximal at 2.5 to 3.5 M NaCl concentration in a complex medium (CM) composed of 1% casamino acids, 1% yeast extract, and NaCl. The addition of 81 mM MgSO4 to CM inhibited nuclease production in spite of good growth. Microscopic observation showed that this inhibition was accompanied by complete clumping of the cells. The Sehgal and Gibbons complex medium (SGC) which contained 0.75% vitamin-free casamino acids, 1% yeast extract, and NaCl, however, supported good production of the nuclease in spite of the presence of 81 mM MgSO4. It seemed that both magnesium sulfate and some substances present in CM might be responsible for this inhibition and clumping. A synthetic medium optimal for enzyme production was developed consisting of 16 amino acids, 4 vitamins, 0.73 mM KH2PO4, 2.7 mM KCl, 20 mM MgSO4, and 2.5 M NaCl. The organism required biotin as an essential growth factor, and thiamine, riboflavin, and choline as stimulating factors. Omission of isoleucine from the medium reduced markedly the growth rate. Glutamic acid, proline, and arginine were consumed completely during cultivation in the synthetic medium.", "PMID": 974905}
{"id": "pubmed23n0032_10036", "title": "Adsorption of Pseudomonas pseudomallei cytophilic antibodies onto rabbit alveolar macrophages.", "content": "Cytophilic antibody has been demonstrated in sera from rabbits immunized with Pseudomonas pseudomallei. This antibody passively adsorbed onto normal rabbit alveolar macrophages rendered the macrophages agglutinable by P. pseudomalei antigens and capable of displaying 'rosette' formation with polysaccharide-sensitized erythrocytes.", "contents": "Adsorption of Pseudomonas pseudomallei cytophilic antibodies onto rabbit alveolar macrophages. Cytophilic antibody has been demonstrated in sera from rabbits immunized with Pseudomonas pseudomallei. This antibody passively adsorbed onto normal rabbit alveolar macrophages rendered the macrophages agglutinable by P. pseudomalei antigens and capable of displaying 'rosette' formation with polysaccharide-sensitized erythrocytes.", "PMID": 974906}
{"id": "pubmed23n0032_10037", "title": "Stability of mRNA from the Clostridium sporogenes phage F1.", "content": "Polyacrylamide gel electrophoresis was used to study the decay of individual species of mRNA in F1, a bacteriophage specific for the obligate anaerobie Clostridium sporogenes. Immediate early mRNA species had a half-life of 3.5 min, while delayed early and late mRNA had a half-life of between 6 and 8 min.", "contents": "Stability of mRNA from the Clostridium sporogenes phage F1. Polyacrylamide gel electrophoresis was used to study the decay of individual species of mRNA in F1, a bacteriophage specific for the obligate anaerobie Clostridium sporogenes. Immediate early mRNA species had a half-life of 3.5 min, while delayed early and late mRNA had a half-life of between 6 and 8 min.", "PMID": 974907}
{"id": "pubmed23n0032_10038", "title": "Physical characteristics of DNA from bacteriophages of Agrobacterium tumefaciens.", "content": "DNA was extracted from isolates of bacteriophages grown on virulent and avirulent strains of Agrobacterium tumefaciens. Molecular weights of DNA from phages isolated from the virulent A. tumefaciens (IIBV7) were about 41.5 X 10(6) daltons, while those from the avirulent A. tumefaciens (IIBNV6) were about 32.5 X 10(6) daltons. The buoyant densities of the four DNA's ranged from 1.7086 to 1.7089 g/cm3, values that were not significantly different. DNA-DNA hybridization studies also indicated that the four phages were closely related. Attempts to induce tumors with phage DNA were unsuccessful.", "contents": "Physical characteristics of DNA from bacteriophages of Agrobacterium tumefaciens. DNA was extracted from isolates of bacteriophages grown on virulent and avirulent strains of Agrobacterium tumefaciens. Molecular weights of DNA from phages isolated from the virulent A. tumefaciens (IIBV7) were about 41.5 X 10(6) daltons, while those from the avirulent A. tumefaciens (IIBNV6) were about 32.5 X 10(6) daltons. The buoyant densities of the four DNA's ranged from 1.7086 to 1.7089 g/cm3, values that were not significantly different. DNA-DNA hybridization studies also indicated that the four phages were closely related. Attempts to induce tumors with phage DNA were unsuccessful.", "PMID": 974908}
{"id": "pubmed23n0032_10039", "title": "Antibacterial activity of marine violet-pigmented Alteromonas with special reference to the production of brominated compounds.", "content": "The synthesis of several types of antibiotics was investigated in four strains of violet-pigmented bacteria belonging to the species Alteromonas luteo-violaceus. Two of the strains simultaneously produce an antibiotic polyanionic polysaccharide, weakly bound to the cells and diffusing throughout the medium, and two intracellular brominated bactericidal substances. The third strain only synthesizes the polyanionic antibiotic. The fourth one is totally inactive. The macromolecular antibiotic, probably responsible for the autointoxication of the bacteria in their cultures, acts at the respiratory level; it induces an increase of oxygen uptake and the production of peroxides by test bacteria. Thus, its activity is inhibited by catalase and peroxidase.", "contents": "Antibacterial activity of marine violet-pigmented Alteromonas with special reference to the production of brominated compounds. The synthesis of several types of antibiotics was investigated in four strains of violet-pigmented bacteria belonging to the species Alteromonas luteo-violaceus. Two of the strains simultaneously produce an antibiotic polyanionic polysaccharide, weakly bound to the cells and diffusing throughout the medium, and two intracellular brominated bactericidal substances. The third strain only synthesizes the polyanionic antibiotic. The fourth one is totally inactive. The macromolecular antibiotic, probably responsible for the autointoxication of the bacteria in their cultures, acts at the respiratory level; it induces an increase of oxygen uptake and the production of peroxides by test bacteria. Thus, its activity is inhibited by catalase and peroxidase.", "PMID": 974909}
{"id": "pubmed23n0032_10040", "title": "Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil.", "content": "The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical observations is described. Best results were obtained if macroconidia were incubated in soil between two Nucleopore membrane filters. Both chlamydospore morphology and lysis, however, were affected to some extent in comparison with that on single membranes. This is probably due to a selective effect on the microflora colonizing the chlamydospores.", "contents": "Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil. The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical observations is described. Best results were obtained if macroconidia were incubated in soil between two Nucleopore membrane filters. Both chlamydospore morphology and lysis, however, were affected to some extent in comparison with that on single membranes. This is probably due to a selective effect on the microflora colonizing the chlamydospores.", "PMID": 974910}
{"id": "pubmed23n0032_10041", "title": "Ultrastructure of forming and dormant chlamydospores of Fusarium solani in soil.", "content": "Chlamydospores of Fusarium solani f. sp. cucurbitae, F. solani f. sp. phaseoli and F. solani f. sp. pisi formed in soil were recovered at intervals and examined by electron microscopy. Cell wall material outside the newly formed chlamydospore cell wall gradually disintegrated. Protoplasts of chlamydospore cells lyse without prior penetration of the cell wall by microorganisms. The mechanisms of lysis of chlamydospores of F. solani in soil are discussed.", "contents": "Ultrastructure of forming and dormant chlamydospores of Fusarium solani in soil. Chlamydospores of Fusarium solani f. sp. cucurbitae, F. solani f. sp. phaseoli and F. solani f. sp. pisi formed in soil were recovered at intervals and examined by electron microscopy. Cell wall material outside the newly formed chlamydospore cell wall gradually disintegrated. Protoplasts of chlamydospore cells lyse without prior penetration of the cell wall by microorganisms. The mechanisms of lysis of chlamydospores of F. solani in soil are discussed.", "PMID": 974911}
{"id": "pubmed23n0032_10042", "title": "The isolation of an infectious A protein--RNA complex from coliphage R17.", "content": "The coprecipitate of A protein and RNA which results from acetic acid treatment of bacteriophage R17 has been shown to form two bands after equilibrium density gradient centrifugation in Cs2SO4. The band of higher density, whose position coincides with that of phage RNA prepared by phenolisation possesses neither A protein nor infectivity while the band of lower density which contains both RNA and A protein is infectious for intact E. coli cells. The nature of the bonding between the A protein and the RNA was also investigated.", "contents": "The isolation of an infectious A protein--RNA complex from coliphage R17. The coprecipitate of A protein and RNA which results from acetic acid treatment of bacteriophage R17 has been shown to form two bands after equilibrium density gradient centrifugation in Cs2SO4. The band of higher density, whose position coincides with that of phage RNA prepared by phenolisation possesses neither A protein nor infectivity while the band of lower density which contains both RNA and A protein is infectious for intact E. coli cells. The nature of the bonding between the A protein and the RNA was also investigated.", "PMID": 974912}
{"id": "pubmed23n0032_10043", "title": "[Degradation of hydrocarbons in the presence of other organic substances by bacteria isolated from seawater].", "content": "Three bacterial strains, isolated on gas-oil from seawater, have a variously changed growth on hexadecane with supply of two organic substances. Acetate reduces growth of all tested strains and particularly the hexadecane degradation by Acinetobacter sp. On the contrary, trypticase-phytone promotes the degradation by the three strains.", "contents": "[Degradation of hydrocarbons in the presence of other organic substances by bacteria isolated from seawater]. Three bacterial strains, isolated on gas-oil from seawater, have a variously changed growth on hexadecane with supply of two organic substances. Acetate reduces growth of all tested strains and particularly the hexadecane degradation by Acinetobacter sp. On the contrary, trypticase-phytone promotes the degradation by the three strains.", "PMID": 974913}
{"id": "pubmed23n0032_10044", "title": "Potash guar gum--a source of halophilic bacteria.", "content": "Moderate and extreme halophilic microorganisms have been isolated from guar gum solutions dissolved in potash brine for use in sylvite froth flotation. Tentative identifications have been made of vibrio, Halobacterium, and Halococcus species. Reinoculation of these isolates into guar gum solutions has shown that they are not carbohydrate-utilizing strains, but instead metabolize the contaminating proteins present in guar.", "contents": "Potash guar gum--a source of halophilic bacteria. Moderate and extreme halophilic microorganisms have been isolated from guar gum solutions dissolved in potash brine for use in sylvite froth flotation. Tentative identifications have been made of vibrio, Halobacterium, and Halococcus species. Reinoculation of these isolates into guar gum solutions has shown that they are not carbohydrate-utilizing strains, but instead metabolize the contaminating proteins present in guar.", "PMID": 974914}
{"id": "pubmed23n0032_10045", "title": "In situ morphologies of deep-sea and sediment bacteria.", "content": "Deep-sea and sediment bacteria at the bottom of an approximately 1200-m water column were sampled by means of pressure vessels attached to a remote underwater manipulator. Cells were immediately fixed in situ with glutaraldehyde, and after processing in the laboratory their morphologies were observed with the scanning electron microscope. Most bacteria were coccoid or rod-lide and less than 0.4 mum in diameter or width. Few filamentous bacteria were observed. Bacteria were in aggregates or free-living. It is concluded that morphologies of deep-sea bacteria collected and fixed at the hydrostatic pressure of their environment are, in general, similar to the observed morphologies of deep-sea bacteria determined at 1 atm pressure after collection and decompression during ascent through the water column.", "contents": "In situ morphologies of deep-sea and sediment bacteria. Deep-sea and sediment bacteria at the bottom of an approximately 1200-m water column were sampled by means of pressure vessels attached to a remote underwater manipulator. Cells were immediately fixed in situ with glutaraldehyde, and after processing in the laboratory their morphologies were observed with the scanning electron microscope. Most bacteria were coccoid or rod-lide and less than 0.4 mum in diameter or width. Few filamentous bacteria were observed. Bacteria were in aggregates or free-living. It is concluded that morphologies of deep-sea bacteria collected and fixed at the hydrostatic pressure of their environment are, in general, similar to the observed morphologies of deep-sea bacteria determined at 1 atm pressure after collection and decompression during ascent through the water column.", "PMID": 974915}
{"id": "pubmed23n0032_10046", "title": "The occurrence of cell-associated enterotoxin B in Staphylococcus aureus.", "content": "Cell-associated enterotoxin B was detected in lysates of cells of Staphylococcus aureus S-6 and 4916 disrupted by sonication or lysostaphin treatment. As much as 67% of this total cell-associated toxin was surface-bound, located outside the cytoplasmic membrane, and was released during protoplasting of this organism by lysostaphin treatment in hypertonic medium. The remainder of the cell-associated toxin was termed cytoplasmic and was released during osmotic lysis of the protoplasts. Levels of cell-associated toxin as a function of the age of the cells showed a rapid increase in both surface-bound, cytoplasmic, and total cell-associated toxin levels during the period of active toxin synthesis (late exponential phase of growth). These cell-associated toxin levels then reached a peak as the culture entered stationary phase, at a time corresponding to a decrease in the rate of toxin synthesis, and decreased slowly thereafter.", "contents": "The occurrence of cell-associated enterotoxin B in Staphylococcus aureus. Cell-associated enterotoxin B was detected in lysates of cells of Staphylococcus aureus S-6 and 4916 disrupted by sonication or lysostaphin treatment. As much as 67% of this total cell-associated toxin was surface-bound, located outside the cytoplasmic membrane, and was released during protoplasting of this organism by lysostaphin treatment in hypertonic medium. The remainder of the cell-associated toxin was termed cytoplasmic and was released during osmotic lysis of the protoplasts. Levels of cell-associated toxin as a function of the age of the cells showed a rapid increase in both surface-bound, cytoplasmic, and total cell-associated toxin levels during the period of active toxin synthesis (late exponential phase of growth). These cell-associated toxin levels then reached a peak as the culture entered stationary phase, at a time corresponding to a decrease in the rate of toxin synthesis, and decreased slowly thereafter.", "PMID": 974916}
{"id": "pubmed23n0032_10047", "title": "Procedures for obtaining sectional views of fungal fructifications by scanning electron microscopy.", "content": "Procedures for sectioning fungal fructifications in host tissues or on artificial media are described, which allow observation of internal structures by scanning electron microscopy. Perithecia of Ceratocystis fimbriata and Phyllachora graminis, and telia of Puccinia xanthii showed excellent preservation of exposed structures in sections which were osmium-coated before being dried. While similar preservation was obtained in sectioned acervuli of Lecanosticta acicola and Marssonia juglandis and in pycnidia of Dothiorella ribis and Phomopsis occulta, the mucilaginous substances produced in these fructifications precluded observation of conidiophores. Extraction of mucilage from these sections was accomplished by periodic acid and dilute KOH treatments, followed by an osmium-coating procedure. In such preparations, mucilage was removed, internal structures were preserved, and pertinent characteristics of conidiogenous cells were resolved.", "contents": "Procedures for obtaining sectional views of fungal fructifications by scanning electron microscopy. Procedures for sectioning fungal fructifications in host tissues or on artificial media are described, which allow observation of internal structures by scanning electron microscopy. Perithecia of Ceratocystis fimbriata and Phyllachora graminis, and telia of Puccinia xanthii showed excellent preservation of exposed structures in sections which were osmium-coated before being dried. While similar preservation was obtained in sectioned acervuli of Lecanosticta acicola and Marssonia juglandis and in pycnidia of Dothiorella ribis and Phomopsis occulta, the mucilaginous substances produced in these fructifications precluded observation of conidiophores. Extraction of mucilage from these sections was accomplished by periodic acid and dilute KOH treatments, followed by an osmium-coating procedure. In such preparations, mucilage was removed, internal structures were preserved, and pertinent characteristics of conidiogenous cells were resolved.", "PMID": 974917}
{"id": "pubmed23n0032_10048", "title": "Sparing effect of lithium ion on the specific requirement for sodium ion for growth of Vibrio parahaemolyticus.", "content": "The role of NaCl in the growth of Vibrio parahaemolyticus strain A-55 was investigated. Maximal growth was obtained at 0.5 M NaCl in a synthetic medium. Na+ could not be replaced for growth by any other cations or substances. When the medium was kept isotonic with 0.5 M NaCl by the addition of sucrose, good growth was obtained with 0.1 M NaCl. By reducing the osmotic pressure and decreasing the NaCl concentration from 0.5 M to 0.1 M, the same growth yield was obtained as when using a medium containing 0.02 M NaCl and 0.08 M LiCl. This was not the case with sucrose. Therefore, it is concluded that ionic strength and osmotic pressure are important environmental factors affecting growth. The minimal essential requirement for Na+ for growth of V. parahaemolyticus was 0.003 M, because this was never replaced with any other cations and agents. Hence, requirement for Na+ for growth involves a specific requirement for Na+, ionic strength, and osmotic pressure, respective concentrations being 0.003 M, 0.047 M, and 0.45 M. Osmotic support was required for growth when the concentration of NaCl was decreased to 0.05 M. The effect of ionic strength of monovalent cations other than Na+ on growth was examined at 0.003 M NaCl. Among LiCl, NH4Cl, KCl, and RbCl, Li+ was the most accelerative for growth in the synthetic medium.", "contents": "Sparing effect of lithium ion on the specific requirement for sodium ion for growth of Vibrio parahaemolyticus. The role of NaCl in the growth of Vibrio parahaemolyticus strain A-55 was investigated. Maximal growth was obtained at 0.5 M NaCl in a synthetic medium. Na+ could not be replaced for growth by any other cations or substances. When the medium was kept isotonic with 0.5 M NaCl by the addition of sucrose, good growth was obtained with 0.1 M NaCl. By reducing the osmotic pressure and decreasing the NaCl concentration from 0.5 M to 0.1 M, the same growth yield was obtained as when using a medium containing 0.02 M NaCl and 0.08 M LiCl. This was not the case with sucrose. Therefore, it is concluded that ionic strength and osmotic pressure are important environmental factors affecting growth. The minimal essential requirement for Na+ for growth of V. parahaemolyticus was 0.003 M, because this was never replaced with any other cations and agents. Hence, requirement for Na+ for growth involves a specific requirement for Na+, ionic strength, and osmotic pressure, respective concentrations being 0.003 M, 0.047 M, and 0.45 M. Osmotic support was required for growth when the concentration of NaCl was decreased to 0.05 M. The effect of ionic strength of monovalent cations other than Na+ on growth was examined at 0.003 M NaCl. Among LiCl, NH4Cl, KCl, and RbCl, Li+ was the most accelerative for growth in the synthetic medium.", "PMID": 974918}
{"id": "pubmed23n0032_10049", "title": "Bacteriorhodopsin formation in Halobacterium halobium.", "content": "Systematic examinations were made of factors influencing bacteriorhodopsin formation during the growth of Halobacterium halobium. Light-induced adenosine triphosphate (ATP) production and [14C]proline uptake were used as measures of functional ability of the purple membrane. Maximum bacteriorhodopsin formation occurred under growth conditions of illumination and limited aeration. The purple membrane -ATP production system did not confer an appreciable growth advantage. Growth in the dark or with adequate aeration partially suppressed bacteriorhodopsin formation and the effects were additive. Nicotine effectively inhibited bacteriorhodopsin formation. A rapid synthesis of functional pigment occurred when washed suspensions of cells which had been grown under illumination with nicotine present were incubated under dark, aerobic conditions. The alleviation of this nicotine inhibition was not blocked by chloramphenicol or bacitracin. Bacteriorhodopsin formation was negligible when washed suspensions of cells from dark, limited aeration or light, adequate aeration cultures were incubated in the light with limited aeration. A nutritionally complex medium was needed to elicit appreciable bacteriorhodopsin formation by the cells from the dark or adequately aerated cultures. Bacitracin partially inhibited this bacteriorhodopsin formation by cells form the light, adequately aerated culture.", "contents": "Bacteriorhodopsin formation in Halobacterium halobium. Systematic examinations were made of factors influencing bacteriorhodopsin formation during the growth of Halobacterium halobium. Light-induced adenosine triphosphate (ATP) production and [14C]proline uptake were used as measures of functional ability of the purple membrane. Maximum bacteriorhodopsin formation occurred under growth conditions of illumination and limited aeration. The purple membrane -ATP production system did not confer an appreciable growth advantage. Growth in the dark or with adequate aeration partially suppressed bacteriorhodopsin formation and the effects were additive. Nicotine effectively inhibited bacteriorhodopsin formation. A rapid synthesis of functional pigment occurred when washed suspensions of cells which had been grown under illumination with nicotine present were incubated under dark, aerobic conditions. The alleviation of this nicotine inhibition was not blocked by chloramphenicol or bacitracin. Bacteriorhodopsin formation was negligible when washed suspensions of cells from dark, limited aeration or light, adequate aeration cultures were incubated in the light with limited aeration. A nutritionally complex medium was needed to elicit appreciable bacteriorhodopsin formation by the cells from the dark or adequately aerated cultures. Bacitracin partially inhibited this bacteriorhodopsin formation by cells form the light, adequately aerated culture.", "PMID": 974919}
{"id": "pubmed23n0032_10050", "title": "Deoxyribonucleic acid studies in the genus Caryophanon.", "content": "The deoxyribonucleic acid (DNA) of 36 isolates of Caryophanon latum and 3 isolates designated Caryophanon tenue, obtained from geographically diverse regions, has been examined for guanine plus cytosine (mol % G+C) base composition, genome size, and for relative reassociation levels. The isolates were found to be homogenous and group-specific in their base composition, with C. latum DNA ranging from 44.0 to 45.6 mol % G+C, and C. tenue from 41.2 to 41.6 mol % G+C. Genome size determined for representative isolates confirmed the distinction of the two groups. The genome size ranged from 1100 to 1200 X 10(6) daltons for C. latum and from 900 to 1000 X 10(6) daltons for C. tenue. DNA:DNA hybridization studies revealed relative reassociation levels as follows: C. latum to C. latum, 78-94%, C. tenue to C. tenue, 82-94%; and C. latum to C. tenue, 13-30%. Finally, based on genome size and mol % G+C similarities, DNA:DNA hybridization experiments between two strains of Lactobacillus and C. latum and C. tenue were performed. These experiments revealed essentially no polynucleotide sequence similarity between the Lactobacillus and Caryophanon isolates used.", "contents": "Deoxyribonucleic acid studies in the genus Caryophanon. The deoxyribonucleic acid (DNA) of 36 isolates of Caryophanon latum and 3 isolates designated Caryophanon tenue, obtained from geographically diverse regions, has been examined for guanine plus cytosine (mol % G+C) base composition, genome size, and for relative reassociation levels. The isolates were found to be homogenous and group-specific in their base composition, with C. latum DNA ranging from 44.0 to 45.6 mol % G+C, and C. tenue from 41.2 to 41.6 mol % G+C. Genome size determined for representative isolates confirmed the distinction of the two groups. The genome size ranged from 1100 to 1200 X 10(6) daltons for C. latum and from 900 to 1000 X 10(6) daltons for C. tenue. DNA:DNA hybridization studies revealed relative reassociation levels as follows: C. latum to C. latum, 78-94%, C. tenue to C. tenue, 82-94%; and C. latum to C. tenue, 13-30%. Finally, based on genome size and mol % G+C similarities, DNA:DNA hybridization experiments between two strains of Lactobacillus and C. latum and C. tenue were performed. These experiments revealed essentially no polynucleotide sequence similarity between the Lactobacillus and Caryophanon isolates used.", "PMID": 974920}
{"id": "pubmed23n0032_10051", "title": "Respiration rates for determining the effects of urea on the soil-surface organic horizon of a black spruce stand.", "content": "The respiration rates of microflora of layers of soil-surface organic horizon of a black spruce (Picea mariana (Mill.) B.S.P.) stand have been studied manometrically under controlled conditions of moisture, temperature, and aeration in the presence of urea and other nitrogen and mineral amendments. L.F., and F2 samples from field plots fertilized with 448 kg N/ha as urea in 1961 had still in 1971 greater respiration rates than similar samples from unfertilized field plots. In lab tests, addition of urea (112, 280, and 448 kg N/ha or 875, 2187, and 3500 ppm N) stimulated the endogenous respiration of each layer. The stimulation was greater when 2187 ppm N was applied and when moisture and temperature of the layers were maintained at 60% water-holding capacity and 20 degrees C during the 42-day incubation period. Addition of Mg, Ca, and K to urea-fertilized layers increased respiration while P and S decreased it. Addition of NH4NO3 and (NH4)2SO4 impaired the endogenous respiration. The endogenous respiration and moisture, temperature, and fertilizer effects decreased in the order L,F1, and F2 layers.", "contents": "Respiration rates for determining the effects of urea on the soil-surface organic horizon of a black spruce stand. The respiration rates of microflora of layers of soil-surface organic horizon of a black spruce (Picea mariana (Mill.) B.S.P.) stand have been studied manometrically under controlled conditions of moisture, temperature, and aeration in the presence of urea and other nitrogen and mineral amendments. L.F., and F2 samples from field plots fertilized with 448 kg N/ha as urea in 1961 had still in 1971 greater respiration rates than similar samples from unfertilized field plots. In lab tests, addition of urea (112, 280, and 448 kg N/ha or 875, 2187, and 3500 ppm N) stimulated the endogenous respiration of each layer. The stimulation was greater when 2187 ppm N was applied and when moisture and temperature of the layers were maintained at 60% water-holding capacity and 20 degrees C during the 42-day incubation period. Addition of Mg, Ca, and K to urea-fertilized layers increased respiration while P and S decreased it. Addition of NH4NO3 and (NH4)2SO4 impaired the endogenous respiration. The endogenous respiration and moisture, temperature, and fertilizer effects decreased in the order L,F1, and F2 layers.", "PMID": 974921}
{"id": "pubmed23n0032_10052", "title": "Bouyant density, conversion formulae, and the mole percent guanosine + cytosine content of Desulfovibrio sp.", "content": "A reevaluation of the original buoyant density conversion formula used to calculate the molar percentage guanosine + cytosine (% G + C) contents of the accepted species of genus Desulfovibrio has been undertaken. It would appear that the formula used gives values 4-5% lower than those obtained using formulae more generally cited in modern literature. Recalculations of % G + C content values for Lesulfovibrio DNA are presented using the formulae of three different workers, and are compared with those values originally used in the classification of genus Desulfovibrio.", "contents": "Bouyant density, conversion formulae, and the mole percent guanosine + cytosine content of Desulfovibrio sp. A reevaluation of the original buoyant density conversion formula used to calculate the molar percentage guanosine + cytosine (% G + C) contents of the accepted species of genus Desulfovibrio has been undertaken. It would appear that the formula used gives values 4-5% lower than those obtained using formulae more generally cited in modern literature. Recalculations of % G + C content values for Lesulfovibrio DNA are presented using the formulae of three different workers, and are compared with those values originally used in the classification of genus Desulfovibrio.", "PMID": 974922}
{"id": "pubmed23n0032_10053", "title": "Water-soluble contrast radiculography of the lumbar region.", "content": "Water-soluble contrast radiculography has been used for many years. Earlier contrast media were highly irritant and subjects required preliminary spinal anesthesia but, recently, better-tolerated contrast media such as meglumine iocarmate (Dimer X) and metrizamide (Amipaque) have been used. The findings from 300 examinations with meglumine iocarmate are presented. Methodology is discussed. The diagnostic accuracy of this contrast medium was high and side effects were minimal. Comment is made on the newest spinal contrast medium--metrizamide--and its use in 29 examinations in Canada.", "contents": "Water-soluble contrast radiculography of the lumbar region. Water-soluble contrast radiculography has been used for many years. Earlier contrast media were highly irritant and subjects required preliminary spinal anesthesia but, recently, better-tolerated contrast media such as meglumine iocarmate (Dimer X) and metrizamide (Amipaque) have been used. The findings from 300 examinations with meglumine iocarmate are presented. Methodology is discussed. The diagnostic accuracy of this contrast medium was high and side effects were minimal. Comment is made on the newest spinal contrast medium--metrizamide--and its use in 29 examinations in Canada.", "PMID": 974925}
{"id": "pubmed23n0032_10054", "title": "Endoscopic retrograde cholangiopancreatography in the management of pancreatic and biliary disease.", "content": "Endoscopic retrograde cholangiopancreatography is a new and valuable technique in the diagnosis of jaundice, pancreatic disease and obscure upper abdominal pain. Endoscopic sphincterotomy of the sphincter of Oddi and the extraction of stones from the common bile duct are an extension of this procedure already in use in several high-volume centres. Cannulation of the bile ducts and pancreatic ducts in tests of secretion and cytology will become increasingly common applications of the technique. In order to provide dependability and cost-effectiveness, specialists in this procedure must maintain a high volume, necessarily limiting it to a few practitioners. Use of a trained endoscopic technician, a radiologist trained in endoscopy, or an endoscopist trained in fluoroscopy and spot-filming may decrease the manpower costs of this procedure.", "contents": "Endoscopic retrograde cholangiopancreatography in the management of pancreatic and biliary disease. Endoscopic retrograde cholangiopancreatography is a new and valuable technique in the diagnosis of jaundice, pancreatic disease and obscure upper abdominal pain. Endoscopic sphincterotomy of the sphincter of Oddi and the extraction of stones from the common bile duct are an extension of this procedure already in use in several high-volume centres. Cannulation of the bile ducts and pancreatic ducts in tests of secretion and cytology will become increasingly common applications of the technique. In order to provide dependability and cost-effectiveness, specialists in this procedure must maintain a high volume, necessarily limiting it to a few practitioners. Use of a trained endoscopic technician, a radiologist trained in endoscopy, or an endoscopist trained in fluoroscopy and spot-filming may decrease the manpower costs of this procedure.", "PMID": 974926}
{"id": "pubmed23n0032_10055", "title": "Acute pancreatitis occurring in heterotopic pancreatic tissue in the gallbladder.", "content": "A 54-year-old man with acute upper abdominal pain was found to have pancreatic heterotopia in the wall of the gallbladder. The aberrant pancreatic tissue and the gallbladder wall were the sites of acute inflammation and of fat necrosis. Pancreatic heterotopia of the gallbladder is extremely rare; only 10 proven cases have been reported previously. The most common sites are the stomach, duodenum and jejunum.", "contents": "Acute pancreatitis occurring in heterotopic pancreatic tissue in the gallbladder. A 54-year-old man with acute upper abdominal pain was found to have pancreatic heterotopia in the wall of the gallbladder. The aberrant pancreatic tissue and the gallbladder wall were the sites of acute inflammation and of fat necrosis. Pancreatic heterotopia of the gallbladder is extremely rare; only 10 proven cases have been reported previously. The most common sites are the stomach, duodenum and jejunum.", "PMID": 974927}
{"id": "pubmed23n0032_10056", "title": "Perforated peptic ulcer in pregnancy with survival of mother and child: case report and review of the literature.", "content": "Peptic ulcer in pregnancy and its complications, though rare, can be life-threatening to mother and fetus. Perforated peptic ulcer in pregnancy is extremely rare; when this occurs survival of both mother and child is unusual. Only five cases of maternal survival following perforation of a peptic ulcer have previously been recorded and, of these, only three were associated with survival of both mother and infant. The authors report an additional case of perforated ulcer in pregnancy with survival of mother and infant, and review the relevant literature. Early surgical diagnosis and treatment followed by vaginal delivery of the fetus offers the best hope for survival of mother and child.", "contents": "Perforated peptic ulcer in pregnancy with survival of mother and child: case report and review of the literature. Peptic ulcer in pregnancy and its complications, though rare, can be life-threatening to mother and fetus. Perforated peptic ulcer in pregnancy is extremely rare; when this occurs survival of both mother and child is unusual. Only five cases of maternal survival following perforation of a peptic ulcer have previously been recorded and, of these, only three were associated with survival of both mother and infant. The authors report an additional case of perforated ulcer in pregnancy with survival of mother and infant, and review the relevant literature. Early surgical diagnosis and treatment followed by vaginal delivery of the fetus offers the best hope for survival of mother and child.", "PMID": 974928}
{"id": "pubmed23n0032_10057", "title": "Immunologic response in vitro after thymechtomy in patients with myasthenia gravis.", "content": "Thymectomy in adult animals impairs immune functions such as lymphocyte response to phytohemagglutinin (PHA) and to allogeneic cells. The responses of lymphocytes from 18 myasthenia gravis patients, 12 of whom had undergone thymectomy, were studied; the interval between thymectomy and investigation ranged from 1 month to 26 years (mean, 9.5 years). Peripheral blood lymphocytes were cultured in autochthonous plasma or homologous AB serum. In vitro responses to stimulation with PHA, concanavalin A and allogeneic monomuclear cells were within the 95% range of normal responses in all patients. Because our findings contrast with the definite immune defects resulting from thymectomy found in adult animals, longer follow-up is needed.", "contents": "Immunologic response in vitro after thymechtomy in patients with myasthenia gravis. Thymectomy in adult animals impairs immune functions such as lymphocyte response to phytohemagglutinin (PHA) and to allogeneic cells. The responses of lymphocytes from 18 myasthenia gravis patients, 12 of whom had undergone thymectomy, were studied; the interval between thymectomy and investigation ranged from 1 month to 26 years (mean, 9.5 years). Peripheral blood lymphocytes were cultured in autochthonous plasma or homologous AB serum. In vitro responses to stimulation with PHA, concanavalin A and allogeneic monomuclear cells were within the 95% range of normal responses in all patients. Because our findings contrast with the definite immune defects resulting from thymectomy found in adult animals, longer follow-up is needed.", "PMID": 974948}
{"id": "pubmed23n0032_10058", "title": "Hyperactives as young adults: preliminary report.", "content": "Thirty-five individuals aged 17 to 24 in whom severe chronic hyperactivity had been diagnosed 10 years before were studied together with 25 matched controls. There were no significant differences in mean height or weight or in electroencephalographic findings, but the mean pulse rate was significantly higher in the hyperactive group. Cognitive style tests indicated continued difficulty in reflection (resulting in more errors) but less impulsivity (longer reaction time) in the hyperactive individuals. Compared with controls, hyperactive subjects were continuing to have more scholastic difficulty, although this difference seemed to be less pronounced than 5 years before. Their adjustment in work situations and living arrangements did not differ significantly from that of the controls. Restlessness, both reported and observed, continued to be a problem for the hyperactive individuals, and socialization skills and sense of well-being continued to be poorer than in the controls. The hyperactive individuals did not show significantly more antisocial behaviour, nonmedical use of drugs or serious psychiatric disturbances.", "contents": "Hyperactives as young adults: preliminary report. Thirty-five individuals aged 17 to 24 in whom severe chronic hyperactivity had been diagnosed 10 years before were studied together with 25 matched controls. There were no significant differences in mean height or weight or in electroencephalographic findings, but the mean pulse rate was significantly higher in the hyperactive group. Cognitive style tests indicated continued difficulty in reflection (resulting in more errors) but less impulsivity (longer reaction time) in the hyperactive individuals. Compared with controls, hyperactive subjects were continuing to have more scholastic difficulty, although this difference seemed to be less pronounced than 5 years before. Their adjustment in work situations and living arrangements did not differ significantly from that of the controls. Restlessness, both reported and observed, continued to be a problem for the hyperactive individuals, and socialization skills and sense of well-being continued to be poorer than in the controls. The hyperactive individuals did not show significantly more antisocial behaviour, nonmedical use of drugs or serious psychiatric disturbances.", "PMID": 974949}
{"id": "pubmed23n0032_10059", "title": "Stabilometry as a diagnostic tool in clinical medicine.", "content": "Stabilomety, a method of measuring stability of stance or postural equilibrium in man, consists of transforming the mechanical oscillations of man's \"physiologic gravicentre\" into electric signals, then amplifying, recording and analysing the signals. The frequency, duration and mean and maximum amplitudes of oscillations, and coefficients reflecting the influence of vision, differ in patients with various neurologic diseases and from values in healthy subjects. The method is highly sensitive and accurate, simple and rapid to use, lacks danger and discomfort and permits screening of a large number of people in a short time.", "contents": "Stabilometry as a diagnostic tool in clinical medicine. Stabilomety, a method of measuring stability of stance or postural equilibrium in man, consists of transforming the mechanical oscillations of man's \"physiologic gravicentre\" into electric signals, then amplifying, recording and analysing the signals. The frequency, duration and mean and maximum amplitudes of oscillations, and coefficients reflecting the influence of vision, differ in patients with various neurologic diseases and from values in healthy subjects. The method is highly sensitive and accurate, simple and rapid to use, lacks danger and discomfort and permits screening of a large number of people in a short time.", "PMID": 974950}
{"id": "pubmed23n0032_10060", "title": "Management of the patient requiring leg amputation for peripheral vascular disease.", "content": "Most leg amputations are performed because of the effects of peripheral vascular disease. Only 50% of such amputations were performed below the knee in Ontario in 1972, although the knee joint should be preserved in most patients. Careful preoperative evaluation is essential. Postoperative problems include gas gangrene for which prophylactic measures are recommended; failure of the stump to heal, which necessitates early revision of the stump; and defects in stump contour, which necessitate late revision. An enthusiastic team approach to rehabilitation is necessary to overcome the physical and mental problems that result from amputation of a leg.", "contents": "Management of the patient requiring leg amputation for peripheral vascular disease. Most leg amputations are performed because of the effects of peripheral vascular disease. Only 50% of such amputations were performed below the knee in Ontario in 1972, although the knee joint should be preserved in most patients. Careful preoperative evaluation is essential. Postoperative problems include gas gangrene for which prophylactic measures are recommended; failure of the stump to heal, which necessitates early revision of the stump; and defects in stump contour, which necessitate late revision. An enthusiastic team approach to rehabilitation is necessary to overcome the physical and mental problems that result from amputation of a leg.", "PMID": 974951}
{"id": "pubmed23n0032_10061", "title": "Mobile hearing program in central rural Manitoba.", "content": "A mobile hearing program was developed to detect and alleviate deafness and rehabilitate deaf individuals in rural Manitoba. The first project was established in central Manitoba in December 1973 for children, whose school performance can be affected by minimal hearing loss. Volunteers from the community, trained by an audiologist, conduct screening procedures, and children with possible hearing impairment receive audiologic and otolaryngologic assessment. Close clinical liaison between audiologist and otologist is important.", "contents": "Mobile hearing program in central rural Manitoba. A mobile hearing program was developed to detect and alleviate deafness and rehabilitate deaf individuals in rural Manitoba. The first project was established in central Manitoba in December 1973 for children, whose school performance can be affected by minimal hearing loss. Volunteers from the community, trained by an audiologist, conduct screening procedures, and children with possible hearing impairment receive audiologic and otolaryngologic assessment. Close clinical liaison between audiologist and otologist is important.", "PMID": 974952}
{"id": "pubmed23n0032_10062", "title": "Marfan syndrome with coronary artery lesions in a North American Indian.", "content": "Marfan syndrome has not been well documented in North American Indians. A 19-year old Ojibwa man had evidence of this syndrome -- specifically, tall stature, long, thin extremities (particularly, fingers and toes), increased urinary excretion of hydroxyproline, aortic aneurysm, aortic regurgitation and pathologic evidence of aortic rupture and alastic tissue fragmentation. Intimal hyperplasia was present in the extramural coronary arteries, while the intramural arteries, usually thought to be involved, were normal.", "contents": "Marfan syndrome with coronary artery lesions in a North American Indian. Marfan syndrome has not been well documented in North American Indians. A 19-year old Ojibwa man had evidence of this syndrome -- specifically, tall stature, long, thin extremities (particularly, fingers and toes), increased urinary excretion of hydroxyproline, aortic aneurysm, aortic regurgitation and pathologic evidence of aortic rupture and alastic tissue fragmentation. Intimal hyperplasia was present in the extramural coronary arteries, while the intramural arteries, usually thought to be involved, were normal.", "PMID": 974953}
{"id": "pubmed23n0032_10063", "title": "Carrier detection and genetic counselling in Duchenne muscular dystrophy: a follow-up study.", "content": "Assay of serum creatine kinase activity is useful in the detection of carriers of the X-linked gene for Duchenne muscular dystrophy (DMD). For genetic counselling this assay has been used in conjunction with pedigree analysis to improve estimates of the risk that a female relative of a DMD patient is a carrier. To measure the impact of the program, follow-up information was obtained from women who had received genetic counselling for DMD. Their responses showed that the risk of producing an affected son had been a major factor in their attitude toward family planning, and their reproductive performance correlated inversely with their genetic risk. The decision by the majority of proven carriers to prevent the birth of further male offspring was reflected in a recent decline in the frequency of a known family history of DMD among newly ascertained cases.", "contents": "Carrier detection and genetic counselling in Duchenne muscular dystrophy: a follow-up study. Assay of serum creatine kinase activity is useful in the detection of carriers of the X-linked gene for Duchenne muscular dystrophy (DMD). For genetic counselling this assay has been used in conjunction with pedigree analysis to improve estimates of the risk that a female relative of a DMD patient is a carrier. To measure the impact of the program, follow-up information was obtained from women who had received genetic counselling for DMD. Their responses showed that the risk of producing an affected son had been a major factor in their attitude toward family planning, and their reproductive performance correlated inversely with their genetic risk. The decision by the majority of proven carriers to prevent the birth of further male offspring was reflected in a recent decline in the frequency of a known family history of DMD among newly ascertained cases.", "PMID": 974964}
{"id": "pubmed23n0032_10064", "title": "Lithium iodide cardiac pacemakers: initial clinical experience.", "content": "A new long-life cardiac pacemaker pulse generator powered by a lithium iodide fuel cell was introduced in Canada in 1973. The compact, hermetically sealed unit is easily implanted and reliable, has excellent patient acceptance and has an anticipated battery life of almost 14 years. Among 105 patients who received a lithium iodide pacemaker, complications occurred in 18. The lithium iodide pacemaker represents a significant advance in pacemaker generator technology and is recommended for long-term cardiac pacing; the manufacturer guarantees the pulse generator for 6 years.", "contents": "Lithium iodide cardiac pacemakers: initial clinical experience. A new long-life cardiac pacemaker pulse generator powered by a lithium iodide fuel cell was introduced in Canada in 1973. The compact, hermetically sealed unit is easily implanted and reliable, has excellent patient acceptance and has an anticipated battery life of almost 14 years. Among 105 patients who received a lithium iodide pacemaker, complications occurred in 18. The lithium iodide pacemaker represents a significant advance in pacemaker generator technology and is recommended for long-term cardiac pacing; the manufacturer guarantees the pulse generator for 6 years.", "PMID": 974965}
{"id": "pubmed23n0032_10065", "title": "Pellagra.", "content": "Pellagra due to malnutrition occurred in an Ottawa women in 1973. She was given a normal diet with supplements of riboflavin, thiamine, niacin and ascorbic acid. The clinical response was striking. Although pellagra is still common in some parts of the world, it is now rare in North America. The disease may occur secondary to malnutrition or malabsorption; inborn errors of metabolism or transport, as in Hartnup disease; deviation of precursors of niacin, as in carcinoid syndrome; or competitive inhibition of niacin incorporation into nicotinamide-adenine dinucleotide, as with administration of isoniazid.", "contents": "Pellagra. Pellagra due to malnutrition occurred in an Ottawa women in 1973. She was given a normal diet with supplements of riboflavin, thiamine, niacin and ascorbic acid. The clinical response was striking. Although pellagra is still common in some parts of the world, it is now rare in North America. The disease may occur secondary to malnutrition or malabsorption; inborn errors of metabolism or transport, as in Hartnup disease; deviation of precursors of niacin, as in carcinoid syndrome; or competitive inhibition of niacin incorporation into nicotinamide-adenine dinucleotide, as with administration of isoniazid.", "PMID": 974966}
{"id": "pubmed23n0032_10066", "title": "Familial aggregation of blood pressure in 558 adopted children.", "content": "Blood pressure was measured in each member of 398 French-Canadian families with at least one adopted child of the same ethnic origin. Measurements were made at home by a nurse, usually with the subject seated. One comparison per family between parents and randomly chosen index children was made, using age- and sex-adjusted scores. The correlation in blood pressure scores between parents and natural children was highly significant (P less than 0.001), at 10.2% for systolic and 13.7% for diastolic in 140 homes with at least one natural child, but the correlation between parents and adopted children was nonsignificant, at 0.81% and 1.0%, in 398 homes with at least one adopted child. The correlation between pairs of natural children was significant (P less than 0.05), at 7.84% for systolic and 8.41% for diastolic, in 80 homes with more than one natural child but nonsignificant, at 0.49% and 1.69%, respectively, for pairs of adopted children in 138 families with more than one adopted child. It is concluded that heredity explains most of the familial resemblance of blood pressure in children.", "contents": "Familial aggregation of blood pressure in 558 adopted children. Blood pressure was measured in each member of 398 French-Canadian families with at least one adopted child of the same ethnic origin. Measurements were made at home by a nurse, usually with the subject seated. One comparison per family between parents and randomly chosen index children was made, using age- and sex-adjusted scores. The correlation in blood pressure scores between parents and natural children was highly significant (P less than 0.001), at 10.2% for systolic and 13.7% for diastolic in 140 homes with at least one natural child, but the correlation between parents and adopted children was nonsignificant, at 0.81% and 1.0%, in 398 homes with at least one adopted child. The correlation between pairs of natural children was significant (P less than 0.05), at 7.84% for systolic and 8.41% for diastolic, in 80 homes with more than one natural child but nonsignificant, at 0.49% and 1.69%, respectively, for pairs of adopted children in 138 families with more than one adopted child. It is concluded that heredity explains most of the familial resemblance of blood pressure in children.", "PMID": 974967}
{"id": "pubmed23n0032_10067", "title": "The Nutrition Canada Survey: a review. Statement by the Nutrition Committee of the Canadian Paediatric Society.", "content": "The data obtained through the Nutrition Canada national nutrition survey must be interpreted cautiously because of limitations in both the design of the survey and our present knowledge of the implications of the findings. The data suggest that, at present, nutritional status is suboptimal among members of the general population. However, because there is no evidence of clinically apparent malnutrition it is difficult to judge the immediate health significance of this finding. Among the Inuit, however, the data suggest that intake of vitamin C may be sufficiently low to approach the level causing clinical problems. Government should make funds available to permit analysis of the data to be completed and investigative studies to be undertaken to assess the health significance of the findings.", "contents": "The Nutrition Canada Survey: a review. Statement by the Nutrition Committee of the Canadian Paediatric Society. The data obtained through the Nutrition Canada national nutrition survey must be interpreted cautiously because of limitations in both the design of the survey and our present knowledge of the implications of the findings. The data suggest that, at present, nutritional status is suboptimal among members of the general population. However, because there is no evidence of clinically apparent malnutrition it is difficult to judge the immediate health significance of this finding. Among the Inuit, however, the data suggest that intake of vitamin C may be sufficiently low to approach the level causing clinical problems. Government should make funds available to permit analysis of the data to be completed and investigative studies to be undertaken to assess the health significance of the findings.", "PMID": 974968}
{"id": "pubmed23n0032_10068", "title": "Mebendazole in the treatment of helminthiasis.", "content": "Mebendazole, a new broad-spectrum anthelmintic, was used to treat patients with nematode infections--ascariasis, trichuriasis and hookworm. The dosage for adults was 100 mg twice daily for 3 days and for children, 50 mg twice daily for 3 days. Pretreatment and post-treatment egg counts on stool specimens showed that after mebendazole there was a reduction of over 99% in egg count per gram of stool in all three types of infection. The overall cure rates for the infections were as follows: Ascaris lumbricoides, 86.8% (59/68); Trichuris trichiura, 86.0% (37/43); and hookworm, 85.7% (24/28). The drug was equally effective in light and heavy infections. No important side effect was noted with this drug. It is suggested that mebendazole is the drug of choice for trichuriasis and mixed nematode infection.", "contents": "Mebendazole in the treatment of helminthiasis. Mebendazole, a new broad-spectrum anthelmintic, was used to treat patients with nematode infections--ascariasis, trichuriasis and hookworm. The dosage for adults was 100 mg twice daily for 3 days and for children, 50 mg twice daily for 3 days. Pretreatment and post-treatment egg counts on stool specimens showed that after mebendazole there was a reduction of over 99% in egg count per gram of stool in all three types of infection. The overall cure rates for the infections were as follows: Ascaris lumbricoides, 86.8% (59/68); Trichuris trichiura, 86.0% (37/43); and hookworm, 85.7% (24/28). The drug was equally effective in light and heavy infections. No important side effect was noted with this drug. It is suggested that mebendazole is the drug of choice for trichuriasis and mixed nematode infection.", "PMID": 974969}
{"id": "pubmed23n0032_10069", "title": "Ten-year results of the treatment of primary operable breast carcinoma: A summary of 304 patients evaluated by the TNM system.", "content": "After all records of patients with breast cancer who received primary treatment at Memorial Hospital in 1960 were reviewed, 304 women with operable, infiltrating carcinoma were identified and classified clinically according to the TNM system of the American Joint Committee for Cancer Staging and End Results Reporting. There were 66 patients (22%) classified under Stage I, 176 (58%) under Stage II, and 62 (20%) under Stage III. There were 82 patients (27%) in whom the nodal status was misclassified clinically. The observed 10-year survival was 59.7%. The 10-year end results (with 95% confidence limits) diminished significantly in relation to advancing clinical stage of disease--90.9% (+/-6.9%) for Stage I, 57.1% (+/-7.3%) for Stage II, and 33.9% (+/-11.8%) for Stage III patients. The 10-year survival in patients with pathologically negative axillary nodes was 71.5%, and in the patients with pathologically positive axillary nodes, 48.3%.", "contents": "Ten-year results of the treatment of primary operable breast carcinoma: A summary of 304 patients evaluated by the TNM system. After all records of patients with breast cancer who received primary treatment at Memorial Hospital in 1960 were reviewed, 304 women with operable, infiltrating carcinoma were identified and classified clinically according to the TNM system of the American Joint Committee for Cancer Staging and End Results Reporting. There were 66 patients (22%) classified under Stage I, 176 (58%) under Stage II, and 62 (20%) under Stage III. There were 82 patients (27%) in whom the nodal status was misclassified clinically. The observed 10-year survival was 59.7%. The 10-year end results (with 95% confidence limits) diminished significantly in relation to advancing clinical stage of disease--90.9% (+/-6.9%) for Stage I, 57.1% (+/-7.3%) for Stage II, and 33.9% (+/-11.8%) for Stage III patients. The 10-year survival in patients with pathologically negative axillary nodes was 71.5%, and in the patients with pathologically positive axillary nodes, 48.3%.", "PMID": 974986}
{"id": "pubmed23n0032_10070", "title": "Should curative surgery be attempted in patients with cancer of the main stem bronchus?", "content": "Among tumors of the lung, main stem bronchial cancer offers a particularly unfavorable prognosis, probably because of its rapid extension to the segmental bronchi, trachea, and extrapulmonary structures. However, analysis of data gathered by the End Results Group indicates that survival among those patients with main stem bronchial cancer whose tumor was resected was virtually the same as that of patients with resectable lung cancer of other sites. From these data it is impossible to assess what role, if any, the resection itself played, and a randomized trial would be necessary to determine whether resection improves the probability of patient survival. On the other hand, it is not now advisable to deny resection to patients with operable main stem bronchial tumors (except oat cell tumors) on the basis of the available nonexperimental evidence alone.", "contents": "Should curative surgery be attempted in patients with cancer of the main stem bronchus? Among tumors of the lung, main stem bronchial cancer offers a particularly unfavorable prognosis, probably because of its rapid extension to the segmental bronchi, trachea, and extrapulmonary structures. However, analysis of data gathered by the End Results Group indicates that survival among those patients with main stem bronchial cancer whose tumor was resected was virtually the same as that of patients with resectable lung cancer of other sites. From these data it is impossible to assess what role, if any, the resection itself played, and a randomized trial would be necessary to determine whether resection improves the probability of patient survival. On the other hand, it is not now advisable to deny resection to patients with operable main stem bronchial tumors (except oat cell tumors) on the basis of the available nonexperimental evidence alone.", "PMID": 974987}
{"id": "pubmed23n0032_10071", "title": "Microinvasive carcinoma of the uterine cervix: A long-term followup study of eighty cases.", "content": "One hundred and eleven patients with microinvasive carcinoma of the uterine cervix were studied over a 21-year period. Thirty-five cases of carcinoma in situ with questionable stromal invasion were excluded. Cases with pathologic examination of less than a cervical cone or the entire cervix were not accepted. The sole pathologic criterion for inclusion was unequivocal invasion to a depth of no more than 5.0 mm. Ninety-one patients were followed for 5 years or until death, and 80 patients for 10 years or until death. One patient was lost to follow-up at 5.5 years. The two deaths officially attributed to cervix cancer prior to 10 years were signed out by nonphysician assistant coroners. Available clinical evidence indicates that at least one of these patients, and probably both, did not die of cervix cancer. From these data, simple hysterectomy would seem to be the maximal treatment indicated. Since the prognosis of microinvasive carcinoma is similar to that of carcinoma in situ, it is suggested that such cases not be included when considering the end results of Stage I cervix cancer.", "contents": "Microinvasive carcinoma of the uterine cervix: A long-term followup study of eighty cases. One hundred and eleven patients with microinvasive carcinoma of the uterine cervix were studied over a 21-year period. Thirty-five cases of carcinoma in situ with questionable stromal invasion were excluded. Cases with pathologic examination of less than a cervical cone or the entire cervix were not accepted. The sole pathologic criterion for inclusion was unequivocal invasion to a depth of no more than 5.0 mm. Ninety-one patients were followed for 5 years or until death, and 80 patients for 10 years or until death. One patient was lost to follow-up at 5.5 years. The two deaths officially attributed to cervix cancer prior to 10 years were signed out by nonphysician assistant coroners. Available clinical evidence indicates that at least one of these patients, and probably both, did not die of cervix cancer. From these data, simple hysterectomy would seem to be the maximal treatment indicated. Since the prognosis of microinvasive carcinoma is similar to that of carcinoma in situ, it is suggested that such cases not be included when considering the end results of Stage I cervix cancer.", "PMID": 974988}
{"id": "pubmed23n0032_10072", "title": "Phase I studies with Baker's Antifol (BAF) (NSC 139105).", "content": "Phase I studies were conducted in 58 adult cancer patients with Baker's Antifol (BAF), a new active-site directed inhibitor of dihydrofolate reductase. Dose escalation ranged from 10 to 250 mg/m2/day X 5 days and courses of treatment were repeated every 2-3 weeks. Biologic effects were observed mostly at doses greater than 100 mg/m2/day X 5 days. The patients developed myelosuppression during 19% of the trials. Other types of toxicity were dermatitis in 12 to 30% and stomatitis in 7 to 38% of the trials. Toxicity was directly related to the impairment of the patient's liver function. Two partial responses (in a patient with adenocarcinoma of the lung and a patient with transitional cell carcinoma of the bladder) occurred. BAF is an active new chemotherapeutic agent which deserves further clinical trials in patients with various malignancies.", "contents": "Phase I studies with Baker's Antifol (BAF) (NSC 139105). Phase I studies were conducted in 58 adult cancer patients with Baker's Antifol (BAF), a new active-site directed inhibitor of dihydrofolate reductase. Dose escalation ranged from 10 to 250 mg/m2/day X 5 days and courses of treatment were repeated every 2-3 weeks. Biologic effects were observed mostly at doses greater than 100 mg/m2/day X 5 days. The patients developed myelosuppression during 19% of the trials. Other types of toxicity were dermatitis in 12 to 30% and stomatitis in 7 to 38% of the trials. Toxicity was directly related to the impairment of the patient's liver function. Two partial responses (in a patient with adenocarcinoma of the lung and a patient with transitional cell carcinoma of the bladder) occurred. BAF is an active new chemotherapeutic agent which deserves further clinical trials in patients with various malignancies.", "PMID": 974989}
{"id": "pubmed23n0032_10073", "title": "Relationship of glycolytic enzyme activities and response of breast cancer patients to chemotherapy: A preliminary report.", "content": "Activities of glucosephosphate isomerase, lactate dehydrogenase, and NADP-isocitrate dehydrogenase were significantly elevated in breast cancer specimens from patients who responded favorably to combination cytotoxic chemotherapy regimens compared with those in carcinomas from patients failing to respond to the same chemotherapy. Presence of estrogen receptors and clinical response to hormonal therapy were also evaluated in neoplasms from these patients. The data suggest that measurement of the enzyme profile, along with estrogen receptor levels, may be useful in selecting a mode of therapy for patients with advanced disease.", "contents": "Relationship of glycolytic enzyme activities and response of breast cancer patients to chemotherapy: A preliminary report. Activities of glucosephosphate isomerase, lactate dehydrogenase, and NADP-isocitrate dehydrogenase were significantly elevated in breast cancer specimens from patients who responded favorably to combination cytotoxic chemotherapy regimens compared with those in carcinomas from patients failing to respond to the same chemotherapy. Presence of estrogen receptors and clinical response to hormonal therapy were also evaluated in neoplasms from these patients. The data suggest that measurement of the enzyme profile, along with estrogen receptor levels, may be useful in selecting a mode of therapy for patients with advanced disease.", "PMID": 974990}
{"id": "pubmed23n0032_10074", "title": "Urinary-bladder toxicity following pelvic irradiation and simultaneous cyclophosphamide therapy.", "content": "The frequency and severity of urinary-bladder toxicity were determined retrospectively in a large series of childhood cancer patients treated with either pelvic irradiation and simultaneous cyclophosphamide or cylclophosphamide with extrapelvic irradiation. Of 50 patients who received the first combination, 17 (34%) developed urinary-bladder toxicity. Eight of the 17 had transient hematuria and dysuria with complete clearing clinically after cessation of treatment; nine had chronic or intermittent hematuria which persisted after treatment was stopped and often resulted in demonstrable fibrosis and telangiectasia of the bladder. By contrast, of 60 children who received cyclophosphamide and radiotherapy outside the pelvic region, only five (8%) developed hematuria and in all instances it was transient. This comparative study demonstrates a significantly increased frequency and severity of urinary-bladder toxicity in cancer patients receiving pelvic irradiation with simultaneous cyclophosphamide.", "contents": "Urinary-bladder toxicity following pelvic irradiation and simultaneous cyclophosphamide therapy. The frequency and severity of urinary-bladder toxicity were determined retrospectively in a large series of childhood cancer patients treated with either pelvic irradiation and simultaneous cyclophosphamide or cylclophosphamide with extrapelvic irradiation. Of 50 patients who received the first combination, 17 (34%) developed urinary-bladder toxicity. Eight of the 17 had transient hematuria and dysuria with complete clearing clinically after cessation of treatment; nine had chronic or intermittent hematuria which persisted after treatment was stopped and often resulted in demonstrable fibrosis and telangiectasia of the bladder. By contrast, of 60 children who received cyclophosphamide and radiotherapy outside the pelvic region, only five (8%) developed hematuria and in all instances it was transient. This comparative study demonstrates a significantly increased frequency and severity of urinary-bladder toxicity in cancer patients receiving pelvic irradiation with simultaneous cyclophosphamide.", "PMID": 974991}
{"id": "pubmed23n0032_10075", "title": "Gastric epithelioid leiomyoma and leiomyosarcoma (leiomyoblastoma).", "content": "A series of 127 surgical specimens of epithelioid leiomyomatous tumors (leiomyoblastomas) of the gastric wall from the files of the Armed Forces Institute of Pathology (AFIP) were studied as to biologic behavior, morphogenesis, and histologic features of value in distinguishing benign and malignant variants. These tumors affect middle-aged men primarily and usually present with upper gastrointestinal bleeding or peptic ulcer-like symptoms. They are composed of a mixture of round epithelioid and spindle cells, many of which have clear cytoplasm. The cells are ensheathed by delicate reticular fibers. The presence of a perithelial or glomoid pattern in some tumors suggests a possible relationship to angiomyoma, glomus tumors, and \"pericytoma.\" The epithelioid leiomyoma, the benign form, often arises in the mid- and distal stomach, especially on the anterior wall. Microscopically, it is recognized by the presence of large epithelioid cells and infrequent mitotic figures. Of 103 epithelioid leiomyomas, only one metastasized and thus was biologically malignant. The epithelioid leiomyosarcoma often arises in the proximal stomach and also distally, especially on the posterior wall. Two histologic types of epithelioid leiomyosarcoma are distinguished from the benign epithelioid leiomyoma by the small size of the cells and occasional higher mitotic counts. One sarcoma variant is a small cell caricature of the leiomyoma. The other is more anaplastic, assoicated with a loss of reticular fibers surrounding the cells and an alveolar arrangement. Epithelioid leiomyosarcomas are the most common type of gastric sarcoma. They are aggressive neoplasms; 63% metastasized, usually within 2 years after diagnosis.", "contents": "Gastric epithelioid leiomyoma and leiomyosarcoma (leiomyoblastoma). A series of 127 surgical specimens of epithelioid leiomyomatous tumors (leiomyoblastomas) of the gastric wall from the files of the Armed Forces Institute of Pathology (AFIP) were studied as to biologic behavior, morphogenesis, and histologic features of value in distinguishing benign and malignant variants. These tumors affect middle-aged men primarily and usually present with upper gastrointestinal bleeding or peptic ulcer-like symptoms. They are composed of a mixture of round epithelioid and spindle cells, many of which have clear cytoplasm. The cells are ensheathed by delicate reticular fibers. The presence of a perithelial or glomoid pattern in some tumors suggests a possible relationship to angiomyoma, glomus tumors, and \"pericytoma.\" The epithelioid leiomyoma, the benign form, often arises in the mid- and distal stomach, especially on the anterior wall. Microscopically, it is recognized by the presence of large epithelioid cells and infrequent mitotic figures. Of 103 epithelioid leiomyomas, only one metastasized and thus was biologically malignant. The epithelioid leiomyosarcoma often arises in the proximal stomach and also distally, especially on the posterior wall. Two histologic types of epithelioid leiomyosarcoma are distinguished from the benign epithelioid leiomyoma by the small size of the cells and occasional higher mitotic counts. One sarcoma variant is a small cell caricature of the leiomyoma. The other is more anaplastic, assoicated with a loss of reticular fibers surrounding the cells and an alveolar arrangement. Epithelioid leiomyosarcomas are the most common type of gastric sarcoma. They are aggressive neoplasms; 63% metastasized, usually within 2 years after diagnosis.", "PMID": 974992}
{"id": "pubmed23n0032_10076", "title": "Infantile fibrosarcoma.", "content": "The pathologic features and the behavior of 53 cases of infantile fibrosarcoma are presented. The tumor nearly always occurred during the first 2 years of life; 41 of the 53 cases occurred during the first, six during the second, and three each during the third and fourth year of life, respectively. Twenty of the 53 cases were present at birth. The tumor was more common in boys (60%) and affected chiefly the distal portions of the lower and upper extremities (72%). Most of the tumors grew rapidly and reached a large size within a few weeks or months. They were generally poorly circumscribed and infiltrated variously subcutaneous fat, muscle, fascia and tendons. Microscopically, they were composed of immature-appearing spindle-shaped cells and were marked by their high cellularity and prominent mitotic activity. Despite these features, follow-up information revealed a favorable clinical course, particularly as compared with the adult form of fibrosarcoma. Of the 48 patients with follow-up data, 31 were alive and well with no evidence of recurrence, eight were alive with recurrence, and one was alive following lobectomy for metastatic tumor. Of the living patients, 12 were treated with amputation, nine with radical or wide local excision, and 15 with simple excision. In two cases surgery was followed by chemotherapy, and in one, by radiotherapy. Eight of the 48 patients with follow-up had died, four of metastatic tumor (8.3%) and four of miscellaneous causes. Wide local excision appears to be the treatment of choice unless the size of the tumor and its anatomic location require amputation. Since late recurrent and metastatic lesions were encountered, long-term followup is necessary before one can safely assume that the patient has been cured.", "contents": "Infantile fibrosarcoma. The pathologic features and the behavior of 53 cases of infantile fibrosarcoma are presented. The tumor nearly always occurred during the first 2 years of life; 41 of the 53 cases occurred during the first, six during the second, and three each during the third and fourth year of life, respectively. Twenty of the 53 cases were present at birth. The tumor was more common in boys (60%) and affected chiefly the distal portions of the lower and upper extremities (72%). Most of the tumors grew rapidly and reached a large size within a few weeks or months. They were generally poorly circumscribed and infiltrated variously subcutaneous fat, muscle, fascia and tendons. Microscopically, they were composed of immature-appearing spindle-shaped cells and were marked by their high cellularity and prominent mitotic activity. Despite these features, follow-up information revealed a favorable clinical course, particularly as compared with the adult form of fibrosarcoma. Of the 48 patients with follow-up data, 31 were alive and well with no evidence of recurrence, eight were alive with recurrence, and one was alive following lobectomy for metastatic tumor. Of the living patients, 12 were treated with amputation, nine with radical or wide local excision, and 15 with simple excision. In two cases surgery was followed by chemotherapy, and in one, by radiotherapy. Eight of the 48 patients with follow-up had died, four of metastatic tumor (8.3%) and four of miscellaneous causes. Wide local excision appears to be the treatment of choice unless the size of the tumor and its anatomic location require amputation. Since late recurrent and metastatic lesions were encountered, long-term followup is necessary before one can safely assume that the patient has been cured.", "PMID": 974993}
{"id": "pubmed23n0032_10077", "title": "The chromosomes and causation of human cancer and leukemia. XIX. Common markers in various tumors.", "content": "Most human cancers are associated with abnormal (marker) chromosomes. In past and present studies banding analysis have uncovered a much larger number of markers in cancer cells than was seen with standard (Giemsa) staining. Furthermore, common markers of identical morphology and origin were found in tumors of related or unrelated tissues or organs, suggesting that in all probability such markers, although present in cancers of diverse nature, may indicate a common etiology, either related to the causation of the cancers, to the progression of the tumors, or to the predilection of certain chromosomes to undergo morphologic changes leading to marker formation. Even though some markers were common to different tumors, the bulk of the markers in the cancers studied could not be identified with certainty and their nature varied from tumor to tumor.", "contents": "The chromosomes and causation of human cancer and leukemia. XIX. Common markers in various tumors. Most human cancers are associated with abnormal (marker) chromosomes. In past and present studies banding analysis have uncovered a much larger number of markers in cancer cells than was seen with standard (Giemsa) staining. Furthermore, common markers of identical morphology and origin were found in tumors of related or unrelated tissues or organs, suggesting that in all probability such markers, although present in cancers of diverse nature, may indicate a common etiology, either related to the causation of the cancers, to the progression of the tumors, or to the predilection of certain chromosomes to undergo morphologic changes leading to marker formation. Even though some markers were common to different tumors, the bulk of the markers in the cancers studied could not be identified with certainty and their nature varied from tumor to tumor.", "PMID": 974994}
{"id": "pubmed23n0032_10078", "title": "Pigmented squamous cell carcinoma of cornea and conjunctiva: A light microscopic, histochemical, and ultrastructural study.", "content": "A light microscopic, histochemical, and ultrastructural study was performed on tissue obtained from a pigmented squamous cell carcinoma of the cornea and conjunctiva. To the best of our knowledge this is the first time that one of these rare pigmented squamous cell carcinomas of the ocular mucous membrane has been described in detail. The occurrence of this neoplasm, which needs to be differentiated from other pigmented lesions, and particularly epibulbar melanomas, underscores the fact that all pigmented tumors are not melanomas. The study revealed that melanin granules, which accounted for the pigmentation of the neoplasm, were present in variable numbers in neoplastic squamous cells, Langerhans cells, macrophages, and stellate cells identified as melanocytes with mature melanosomes. Premelanosomes were not observed in any of these cells types. Current concepts concerning the common association of Langerhans cells with their characteristic intracytoplasmic \"Birbeck granules\" and melanocytes are discussed.", "contents": "Pigmented squamous cell carcinoma of cornea and conjunctiva: A light microscopic, histochemical, and ultrastructural study. A light microscopic, histochemical, and ultrastructural study was performed on tissue obtained from a pigmented squamous cell carcinoma of the cornea and conjunctiva. To the best of our knowledge this is the first time that one of these rare pigmented squamous cell carcinomas of the ocular mucous membrane has been described in detail. The occurrence of this neoplasm, which needs to be differentiated from other pigmented lesions, and particularly epibulbar melanomas, underscores the fact that all pigmented tumors are not melanomas. The study revealed that melanin granules, which accounted for the pigmentation of the neoplasm, were present in variable numbers in neoplastic squamous cells, Langerhans cells, macrophages, and stellate cells identified as melanocytes with mature melanosomes. Premelanosomes were not observed in any of these cells types. Current concepts concerning the common association of Langerhans cells with their characteristic intracytoplasmic \"Birbeck granules\" and melanocytes are discussed.", "PMID": 974995}
{"id": "pubmed23n0032_10079", "title": "Pulmonary blastoma: A light and electron microscopic study.", "content": "A case of pulmonary blastoma studied with the electron microscope showed two distinct cell types, i.e., epithelial and mesenchymal elements. The former demonstrates the differentiation toward ciliated bronchial cells, and the latter, cartilage. In view of these findings, coupled with the current understanding of pulmonary morphogenesis, the pulmonary blastoma is more likely to be a carcinosarcoma than a true blastoma. However, the separation between pulmonary blastoma as usually employed and the so-called pulmonary carcinosarcoma seems to be desirable because of different clinicopathologic features.", "contents": "Pulmonary blastoma: A light and electron microscopic study. A case of pulmonary blastoma studied with the electron microscope showed two distinct cell types, i.e., epithelial and mesenchymal elements. The former demonstrates the differentiation toward ciliated bronchial cells, and the latter, cartilage. In view of these findings, coupled with the current understanding of pulmonary morphogenesis, the pulmonary blastoma is more likely to be a carcinosarcoma than a true blastoma. However, the separation between pulmonary blastoma as usually employed and the so-called pulmonary carcinosarcoma seems to be desirable because of different clinicopathologic features.", "PMID": 974996}
{"id": "pubmed23n0032_10080", "title": "Ultrastructural characteristics of sinus histiocytic reaction in lymph nodes draining various stages of breast cancer.", "content": "An ultrastructural study of sinus histiocytosis (SH) in axillary lymph nodes was undertaken in 20 patients with primary cancer of the breast. The lymph node responses were first classified by means of light microscopy, according to previously established criteria. The ultrastructural findings established three major patterns of SH response in cancer-draining lymph nodes: developing SH, positive SH, and degenerative SH. These patterns followed closely the classification obtained by light microscopy. An important finding of electron microscopy was the presence of early activation of histiocytes (developing SH) in microscopy (negative SH). The striking ultrastructural resemblance of histiocytes in positive SH to epithelioid cells seen in delayed hypersensitivity reactions (TB) again points to the fact that the positive SH pattern represents a tumor-host reaction of the delayed hypersensitivity type. Finally, although the presence of virus has been shown to play a role in spontaneous BALB/c murine breast tumor, no virions could be found in human cancer-draining lymph nodes.", "contents": "Ultrastructural characteristics of sinus histiocytic reaction in lymph nodes draining various stages of breast cancer. An ultrastructural study of sinus histiocytosis (SH) in axillary lymph nodes was undertaken in 20 patients with primary cancer of the breast. The lymph node responses were first classified by means of light microscopy, according to previously established criteria. The ultrastructural findings established three major patterns of SH response in cancer-draining lymph nodes: developing SH, positive SH, and degenerative SH. These patterns followed closely the classification obtained by light microscopy. An important finding of electron microscopy was the presence of early activation of histiocytes (developing SH) in microscopy (negative SH). The striking ultrastructural resemblance of histiocytes in positive SH to epithelioid cells seen in delayed hypersensitivity reactions (TB) again points to the fact that the positive SH pattern represents a tumor-host reaction of the delayed hypersensitivity type. Finally, although the presence of virus has been shown to play a role in spontaneous BALB/c murine breast tumor, no virions could be found in human cancer-draining lymph nodes.", "PMID": 974997}
{"id": "pubmed23n0032_10081", "title": "Blastomatous tumors of the respiratory tract.", "content": "Two cases of blastomatous tumors of the respiratory tract are presented. The first is a pulmonary blastoma of an 81-year-old man, diagnosed as adenocarcinoma by cytologic examination, the cells being exfoliated from the large carcinomatous component. The patient died 1 year after manifestation of the symptoms. The second case is a tumor that developed in the nasopharynx of a 62-year-old man. This is the first reported case of a nasopharyngeal blastoma that presented a histology comparable to that seen in the pulmonary tumor. The presence of a hamartomatous benign mesenchymal component raises histogenetic considerations as to whether this was an independent part of an otherwise malignant tumor or whether it was induced by the malignant growth. Following incomplete surgical treatment and postoperative radiation, no recurrence was observed during the next 8 months.", "contents": "Blastomatous tumors of the respiratory tract. Two cases of blastomatous tumors of the respiratory tract are presented. The first is a pulmonary blastoma of an 81-year-old man, diagnosed as adenocarcinoma by cytologic examination, the cells being exfoliated from the large carcinomatous component. The patient died 1 year after manifestation of the symptoms. The second case is a tumor that developed in the nasopharynx of a 62-year-old man. This is the first reported case of a nasopharyngeal blastoma that presented a histology comparable to that seen in the pulmonary tumor. The presence of a hamartomatous benign mesenchymal component raises histogenetic considerations as to whether this was an independent part of an otherwise malignant tumor or whether it was induced by the malignant growth. Following incomplete surgical treatment and postoperative radiation, no recurrence was observed during the next 8 months.", "PMID": 974998}
{"id": "pubmed23n0032_10082", "title": "Structure of psammoma bodies of a meningioma in scanning electron microscopy.", "content": "Psammoma bodies in a human transitional meningioma were studied by scanning electron microscopy (SEM) of deparaffinized tissue slices and sections. The bodies, visible as round structures by light microscopy, were confirmed in SEM to have a spherical rather than tubular three-dimensional structure. They were distinctly demarcated from a surrounding cup-like wall of cells. Under high magnification, the undulating surface of the bodies was found to be covered by interwoven fibers. In cross-section the bodies had a lamellar internal structure. The lamellae consisted of a network of concentric strands and fibers with associated bead-like material. The structure of psammoma bodies seen in SEM speaks in favor of their genesis by calcification of concentrically deposited fibrillar extracellular material.", "contents": "Structure of psammoma bodies of a meningioma in scanning electron microscopy. Psammoma bodies in a human transitional meningioma were studied by scanning electron microscopy (SEM) of deparaffinized tissue slices and sections. The bodies, visible as round structures by light microscopy, were confirmed in SEM to have a spherical rather than tubular three-dimensional structure. They were distinctly demarcated from a surrounding cup-like wall of cells. Under high magnification, the undulating surface of the bodies was found to be covered by interwoven fibers. In cross-section the bodies had a lamellar internal structure. The lamellae consisted of a network of concentric strands and fibers with associated bead-like material. The structure of psammoma bodies seen in SEM speaks in favor of their genesis by calcification of concentrically deposited fibrillar extracellular material.", "PMID": 974999}
{"id": "pubmed23n0032_10083", "title": "Acinic cell tumor of the bronchus.", "content": "A bronchogenic tumor was removed from a 12-year-old girl. Histology showed a clear cell lesion with electron microscope features compatible with serous acinic cell origin.", "contents": "Acinic cell tumor of the bronchus. A bronchogenic tumor was removed from a 12-year-old girl. Histology showed a clear cell lesion with electron microscope features compatible with serous acinic cell origin.", "PMID": 975000}
{"id": "pubmed23n0032_10084", "title": "Erythrokinetics and androgens in bone marrow cancer.", "content": "Erythrokinetic studies were conducted in 60 patients with chronic anemia due to bone marrow involvement by cancer and other diseases. Fifty-seven percent had evidence of ineffective erythropoiesis contributing to the severity of their anemia. Enhanced bone marrow activity in the extremities was detected in 14% of patients. Maximal doses of several oral androgens were given to 31 patients for at least 3 months. Only 26% of the patients increased their red cell mass by at least 20%. All who benefitted had a minimum level of effective erythropoiesis and showed a decline in serum iron concentration at least 30 mug/100 ml after 1 month of treatment. Erythropoietin values were not helpful in identifying responding patients. These findings indicated the value of erythrokinetic studies in defining the bone marrow capacity of patients with cancer who are considered for androgen treatment trials.", "contents": "Erythrokinetics and androgens in bone marrow cancer. Erythrokinetic studies were conducted in 60 patients with chronic anemia due to bone marrow involvement by cancer and other diseases. Fifty-seven percent had evidence of ineffective erythropoiesis contributing to the severity of their anemia. Enhanced bone marrow activity in the extremities was detected in 14% of patients. Maximal doses of several oral androgens were given to 31 patients for at least 3 months. Only 26% of the patients increased their red cell mass by at least 20%. All who benefitted had a minimum level of effective erythropoiesis and showed a decline in serum iron concentration at least 30 mug/100 ml after 1 month of treatment. Erythropoietin values were not helpful in identifying responding patients. These findings indicated the value of erythrokinetic studies in defining the bone marrow capacity of patients with cancer who are considered for androgen treatment trials.", "PMID": 975001}
{"id": "pubmed23n0032_10085", "title": "Lymphomatoid granulomatosis: A clinicopathologic study of four cases.", "content": "Four cases of lymphomatoid granulomatosis were studied. One case, previously reported, has had a prolonged remission of 8 years' duration. In one case, the course was rapid and progressive, and the patient died 2 months after the onset of the disease. In two other patients, the disease appears to be arrested effectively, both clinically and radiographically, by administration of corticosteroids. In the fatal case, postmortem examination revealed a typical angiocentric and destructive polymorphous lymphoreticular infiltrate in the lungs, kidneys, and adrenal glands. The diagnosis was made on specimens obtained from three patients by open thoracotomy. An adequate specimen is mandatory for diagnosis and thoractomy is indicated. To be considered in the differential diagnosis are Wegener's granulomatosis, the limited form of Wegener's granulomatosis, lymphoma, allergic granulomatosis, lymphocytic interstitial pneumonia, plasma cell granuloma, and infectious granuloma.", "contents": "Lymphomatoid granulomatosis: A clinicopathologic study of four cases. Four cases of lymphomatoid granulomatosis were studied. One case, previously reported, has had a prolonged remission of 8 years' duration. In one case, the course was rapid and progressive, and the patient died 2 months after the onset of the disease. In two other patients, the disease appears to be arrested effectively, both clinically and radiographically, by administration of corticosteroids. In the fatal case, postmortem examination revealed a typical angiocentric and destructive polymorphous lymphoreticular infiltrate in the lungs, kidneys, and adrenal glands. The diagnosis was made on specimens obtained from three patients by open thoracotomy. An adequate specimen is mandatory for diagnosis and thoractomy is indicated. To be considered in the differential diagnosis are Wegener's granulomatosis, the limited form of Wegener's granulomatosis, lymphoma, allergic granulomatosis, lymphocytic interstitial pneumonia, plasma cell granuloma, and infectious granuloma.", "PMID": 975002}
{"id": "pubmed23n0032_10086", "title": "Pathophysiology of hypoalbuminemia associated with carcinoid tumor.", "content": "Decreased serum albumin levels are commonly observed in patients with carcinoid tumor, who also show several characteristic clinical and biochemical abnormalities. A large comparative study on a group of 96 carcinoid patients was performed with the purpose of identifying some of the mechanisms leading to hypoalbuminemia in patients with this form of cancer, and thereby to shed light on the cause of hypoalbuminemia of cancer in general. Serum albumin values were compared with a number of clinical parameters (including extent of liver metastases, severity of diarrhea, degree of right heart failure, and extent of gastrointestinal surgery) and of laboratory data (prothrombin time, BSP retention, serum transferrin concentration, hematocrit value, and daily urine excretion of 5-hydroxy-indoleacetic acid). In several patients the gastrointestinal protein loss was assessed by the 51Cr-albumin technique, whereas albumin renewal and distribution were evaluated by the use of 125I-albumin. The data obtained showed that the main factors in determining decreased serum albumin levels in patients with carcinoids are both reduced synthesis and increased loss of the protein. The hepatic synthetic defect appears to be related to a progressive decrease in the number of functioning liver cells; the origin of the gastrointestinal protein loss may be related to the obvious tumor involvement of the gut wall, as well as to the pharmacologically-induced diarrhea. Right heart failure occurring as a result of the carcinoid heart disease may be an additional cause for gastrointestinal protein loss in patients with carcinoid tumor.", "contents": "Pathophysiology of hypoalbuminemia associated with carcinoid tumor. Decreased serum albumin levels are commonly observed in patients with carcinoid tumor, who also show several characteristic clinical and biochemical abnormalities. A large comparative study on a group of 96 carcinoid patients was performed with the purpose of identifying some of the mechanisms leading to hypoalbuminemia in patients with this form of cancer, and thereby to shed light on the cause of hypoalbuminemia of cancer in general. Serum albumin values were compared with a number of clinical parameters (including extent of liver metastases, severity of diarrhea, degree of right heart failure, and extent of gastrointestinal surgery) and of laboratory data (prothrombin time, BSP retention, serum transferrin concentration, hematocrit value, and daily urine excretion of 5-hydroxy-indoleacetic acid). In several patients the gastrointestinal protein loss was assessed by the 51Cr-albumin technique, whereas albumin renewal and distribution were evaluated by the use of 125I-albumin. The data obtained showed that the main factors in determining decreased serum albumin levels in patients with carcinoids are both reduced synthesis and increased loss of the protein. The hepatic synthetic defect appears to be related to a progressive decrease in the number of functioning liver cells; the origin of the gastrointestinal protein loss may be related to the obvious tumor involvement of the gut wall, as well as to the pharmacologically-induced diarrhea. Right heart failure occurring as a result of the carcinoid heart disease may be an additional cause for gastrointestinal protein loss in patients with carcinoid tumor.", "PMID": 975003}
{"id": "pubmed23n0032_10087", "title": "A demonstration of androgen and estrogen receptors in a human breast cancer using a new protamine sulfate assay.", "content": "Separate receptors for estrogen and androgen are demonstrated in cells from metastic human breast cancer. By criteria of binding affinity, number of binding sites, and specificity of the receptor for different steroids, the receptors are shown to be distinguishable. The protamine sulfate receptor assay technique employed allows both kinds of receptor to be quantified conveniently and reproducibly without interference by plasma steroid-binding components.", "contents": "A demonstration of androgen and estrogen receptors in a human breast cancer using a new protamine sulfate assay. Separate receptors for estrogen and androgen are demonstrated in cells from metastic human breast cancer. By criteria of binding affinity, number of binding sites, and specificity of the receptor for different steroids, the receptors are shown to be distinguishable. The protamine sulfate receptor assay technique employed allows both kinds of receptor to be quantified conveniently and reproducibly without interference by plasma steroid-binding components.", "PMID": 975004}
{"id": "pubmed23n0032_10088", "title": "Tumors of fibrous tissue origin in childhood. A clinicopathologic study of cutaneous and soft tissue neoplasms in 66 children.", "content": "Fibroblastic lesions in children present difficulties in diagnosis and in a justifiable therapeutic approach. This series reviews the clinical and pathologic features of fibroblastic soft tissue lesions from 66 children. Although radical procedures were occasionally required to achieve local control of an aggressive lesion, conservative surgery was generally successful. Recurrences were frequent, but only one lesion, in an adolescent, metastasized. No prognostic value could be found in anatomic location or in histologic features. Even cellular lesions with frequent mitoses ordinarily did not require the aggressive therapy needed in similar lesions in adults. In this series, incomplete original excision was frequent and usually related to the infiltrating margins of the lesion.", "contents": "Tumors of fibrous tissue origin in childhood. A clinicopathologic study of cutaneous and soft tissue neoplasms in 66 children. Fibroblastic lesions in children present difficulties in diagnosis and in a justifiable therapeutic approach. This series reviews the clinical and pathologic features of fibroblastic soft tissue lesions from 66 children. Although radical procedures were occasionally required to achieve local control of an aggressive lesion, conservative surgery was generally successful. Recurrences were frequent, but only one lesion, in an adolescent, metastasized. No prognostic value could be found in anatomic location or in histologic features. Even cellular lesions with frequent mitoses ordinarily did not require the aggressive therapy needed in similar lesions in adults. In this series, incomplete original excision was frequent and usually related to the infiltrating margins of the lesion.", "PMID": 975005}
{"id": "pubmed23n0032_10089", "title": "Proximal tubular adenomas of kidney with so-called oncocytic features. A clinicopathologic study of 13 cases of a rarely reported neoplasm.", "content": "Four renal \"oncocytomas\" were observed between July 1974 and January 1975 at University Hospital. A review of all renal cell neoplasms previously classified as carcinomas since 1952 was conducted to determine whether this cluster of tumors represented a recent increase in incidence or whether other renal \"oncocytomas\" had gone unrecognized in the past. The 23-year review of 194 carcinomas yielded an additional nine \"oncocytomas.\" None had appeared prior to 1964, whereas almost half were found in the 1973-1975 interval alone. While it appears that some \"oncocytomas\" of the kidney have gone unrecognized in the past, it is also evident that their recent increased incidence is epidemiologically significant. Renal \"oncocytomas\" and renal cell carcinomas are compared with respect to morphology, clinical presentation, and biologic behavior. Evidence derived from this comparison permits the conclusion that these neoplasms originate from proximal tubular epithelium, are benign clinicopathologic entities, and henceforth should be called proximal tubular adenomas with so-called \"oncocytic\" features.", "contents": "Proximal tubular adenomas of kidney with so-called oncocytic features. A clinicopathologic study of 13 cases of a rarely reported neoplasm. Four renal \"oncocytomas\" were observed between July 1974 and January 1975 at University Hospital. A review of all renal cell neoplasms previously classified as carcinomas since 1952 was conducted to determine whether this cluster of tumors represented a recent increase in incidence or whether other renal \"oncocytomas\" had gone unrecognized in the past. The 23-year review of 194 carcinomas yielded an additional nine \"oncocytomas.\" None had appeared prior to 1964, whereas almost half were found in the 1973-1975 interval alone. While it appears that some \"oncocytomas\" of the kidney have gone unrecognized in the past, it is also evident that their recent increased incidence is epidemiologically significant. Renal \"oncocytomas\" and renal cell carcinomas are compared with respect to morphology, clinical presentation, and biologic behavior. Evidence derived from this comparison permits the conclusion that these neoplasms originate from proximal tubular epithelium, are benign clinicopathologic entities, and henceforth should be called proximal tubular adenomas with so-called \"oncocytic\" features.", "PMID": 975006}
{"id": "pubmed23n0032_10090", "title": "Diffuse cystic glandular malformation of the stomach associated with adenocarcinoma: Case report and review of the literature.", "content": "A 60-year-old man was found to have diffuse cystic glandular malformation and adenocarcinoma of the stomach. Review of the literature revealed one similar reported case and five others with diffuse heterotopic cystic malformation only. Clinically, the lesion is associated with gastrointestinal symptoms, and radiologically, it can mimic diffuse gastric carcinoma, lymphoma, and polyposis.", "contents": "Diffuse cystic glandular malformation of the stomach associated with adenocarcinoma: Case report and review of the literature. A 60-year-old man was found to have diffuse cystic glandular malformation and adenocarcinoma of the stomach. Review of the literature revealed one similar reported case and five others with diffuse heterotopic cystic malformation only. Clinically, the lesion is associated with gastrointestinal symptoms, and radiologically, it can mimic diffuse gastric carcinoma, lymphoma, and polyposis.", "PMID": 975007}
{"id": "pubmed23n0032_10091", "title": "Tumor imaging in children.", "content": "Twnty-six children suspected of having malignant diseases were examined by Gallium-67 scintigraphy. The scintigrams were pathologic in the 20 children with final diagnoses of malignancy. The lesions visualized by scintigraphy were 12 primary tumors and eight cases of local recurrence or dissemination of cancer. Gallium-67 did not accumulate in a hematoma of the liver, and scintigraphy was normal in five children with no clinical evidence of tumor recurrence. Several cases are reported and the clinical value of 67Ca scintigraphy for detection and control of childhood neoplasms is emphasized.", "contents": "Tumor imaging in children. Twnty-six children suspected of having malignant diseases were examined by Gallium-67 scintigraphy. The scintigrams were pathologic in the 20 children with final diagnoses of malignancy. The lesions visualized by scintigraphy were 12 primary tumors and eight cases of local recurrence or dissemination of cancer. Gallium-67 did not accumulate in a hematoma of the liver, and scintigraphy was normal in five children with no clinical evidence of tumor recurrence. Several cases are reported and the clinical value of 67Ca scintigraphy for detection and control of childhood neoplasms is emphasized.", "PMID": 975008}
{"id": "pubmed23n0032_10092", "title": "Bilateral breast carcinomas: Role of mammography.", "content": "From 1963 to 1973, at Emory Clinic, out of a total of 1112 patients with breast carcinoma studied with mammography, 83 had carcinoma in both breasts. The carcinoma in the second breast was primary in 67 patients and metastatic in 16 patients; in 18 patients there were simultaneous bilateral primary carcinomas. Mammography proved highly effective in detecting the second carcinoma and was reliable in differentiating a second primary from metastatic carcinoma. Forty-one of the second primary carcinomas were not associated with a palpable mass; 31 of these were having mammography as a routine check-up. The second nonsimultaneous carcinoma was considerably smaller than the first and fewer axillary lymph node metastasis. The second primary occurred within 6 years of the first in 86% of the cases with the remaining 14% scattered evenly up to 23 years. At 4 years after the diagnosis of the second primary, 25 of 27 deaths had occurred and only 1 patient was alive with cancer. Breast cancer patients need close follow-up for at least 6 years after the first primary carcinoma; and 4 years past the second primary signals a more optimistic prognosis.", "contents": "Bilateral breast carcinomas: Role of mammography. From 1963 to 1973, at Emory Clinic, out of a total of 1112 patients with breast carcinoma studied with mammography, 83 had carcinoma in both breasts. The carcinoma in the second breast was primary in 67 patients and metastatic in 16 patients; in 18 patients there were simultaneous bilateral primary carcinomas. Mammography proved highly effective in detecting the second carcinoma and was reliable in differentiating a second primary from metastatic carcinoma. Forty-one of the second primary carcinomas were not associated with a palpable mass; 31 of these were having mammography as a routine check-up. The second nonsimultaneous carcinoma was considerably smaller than the first and fewer axillary lymph node metastasis. The second primary occurred within 6 years of the first in 86% of the cases with the remaining 14% scattered evenly up to 23 years. At 4 years after the diagnosis of the second primary, 25 of 27 deaths had occurred and only 1 patient was alive with cancer. Breast cancer patients need close follow-up for at least 6 years after the first primary carcinoma; and 4 years past the second primary signals a more optimistic prognosis.", "PMID": 975009}
{"id": "pubmed23n0032_10093", "title": "Primary extramedullary esophageal plasmacytoma: First case report.", "content": "Solitary extramedullary plasmacytomas are rare tumors, reported to occur most commonly in the upper respiratory passages, but which also are rarely seen in the middle and lower gastrointestinal system. This report documents the first primary plasmacytoma of the esophagus. A 67-year-old Caucasian man complained of dysphagia and weight loss. Preoperative diagnosis, based on functional inquiry, radiology, and biopsy, was undifferentiated carcinoma involving the lower third of esophagus. The patient was treated by esophagogastrectomy. The pathology specimen revealed the typical histology of plasmacytoma. The extramedullary nature of the tumor was established postoperatively on the basis of the following criteria: 1) Absence of Bence-Jones proteinurea; 2) normal serum electrophoresis; 3) normal bone marrow biopsy; and 4) absence of distant metastases on liver scan and bone survey.", "contents": "Primary extramedullary esophageal plasmacytoma: First case report. Solitary extramedullary plasmacytomas are rare tumors, reported to occur most commonly in the upper respiratory passages, but which also are rarely seen in the middle and lower gastrointestinal system. This report documents the first primary plasmacytoma of the esophagus. A 67-year-old Caucasian man complained of dysphagia and weight loss. Preoperative diagnosis, based on functional inquiry, radiology, and biopsy, was undifferentiated carcinoma involving the lower third of esophagus. The patient was treated by esophagogastrectomy. The pathology specimen revealed the typical histology of plasmacytoma. The extramedullary nature of the tumor was established postoperatively on the basis of the following criteria: 1) Absence of Bence-Jones proteinurea; 2) normal serum electrophoresis; 3) normal bone marrow biopsy; and 4) absence of distant metastases on liver scan and bone survey.", "PMID": 975010}
{"id": "pubmed23n0032_10094", "title": "Diagnostic scalene node biopsy in the preoperative evaluation of the surgical cancer patient: Five-year experience with 108 cases and literature review.", "content": "Records were reviewed of 108 consecutive patients with a variety of surgically approachable malignancies undergoing scalene node biopsy as part of a preoperative staging evaluation. Metastases to the scalene nodes were detected in 12 patients (11.1%). In six patients the scalene nodes were clinically suspicious before biopsy, but in six with nonpalpable nodes the scalene metastases were occult clinically. Four of the patients with clinically benign, but pathologically positive, scalene biopsies were found among the group of 62 patients with epidermoid carcinoma of the cervix. It was concluded that routine scalene biopsies are justified in the properative evaluation of cancer patients only if the nodes are clinically suspicious, except for in patients with advanced pelvic cancers, in whom the incidence of completely occult scalene metastases is 6% or more.", "contents": "Diagnostic scalene node biopsy in the preoperative evaluation of the surgical cancer patient: Five-year experience with 108 cases and literature review. Records were reviewed of 108 consecutive patients with a variety of surgically approachable malignancies undergoing scalene node biopsy as part of a preoperative staging evaluation. Metastases to the scalene nodes were detected in 12 patients (11.1%). In six patients the scalene nodes were clinically suspicious before biopsy, but in six with nonpalpable nodes the scalene metastases were occult clinically. Four of the patients with clinically benign, but pathologically positive, scalene biopsies were found among the group of 62 patients with epidermoid carcinoma of the cervix. It was concluded that routine scalene biopsies are justified in the properative evaluation of cancer patients only if the nodes are clinically suspicious, except for in patients with advanced pelvic cancers, in whom the incidence of completely occult scalene metastases is 6% or more.", "PMID": 975011}
{"id": "pubmed23n0032_10095", "title": "Abdominoperineal resection: Treatment of choice for carcinoma of the rectum.", "content": "Abdominoperineal resection cure adenocarcinoma of the rectum was performed in 62 patients between 1965 and 1969 at the Winnipeg General Hospital. Mean age was 64, ranging from 41 to 83; 40 patients were male and 22 female. Distribution by Dukes' staging was: A, 11; B, 28; C, 23. Complications occurred in 22 patients (35%). Average hospital stay was 29 days, 27 days in uncomplicated patients and 36 days in those with complications. There were two deaths, a 3.2% operative mortality rate. Sixty of the 62 patients were available for 5-year followup. Crude survival rate was 52%: A, 91%; B, 59%; C, 25%. Two patients died of an operation that permanently cured three patients in whom disease had spread beyond local confines. Abdominoperineal resection offered a 52% 5-year survival rate, increased the rate of cures in the unstaged patient by at least 5% over that afforded by local therapy, gave a 14% chance of cure in stage C disease compared with 0% with local therapy, and, compared with local therapy, was at least 67% more likely to offer cure than to kill in the event of disease spread to regional lymph nodes.", "contents": "Abdominoperineal resection: Treatment of choice for carcinoma of the rectum. Abdominoperineal resection cure adenocarcinoma of the rectum was performed in 62 patients between 1965 and 1969 at the Winnipeg General Hospital. Mean age was 64, ranging from 41 to 83; 40 patients were male and 22 female. Distribution by Dukes' staging was: A, 11; B, 28; C, 23. Complications occurred in 22 patients (35%). Average hospital stay was 29 days, 27 days in uncomplicated patients and 36 days in those with complications. There were two deaths, a 3.2% operative mortality rate. Sixty of the 62 patients were available for 5-year followup. Crude survival rate was 52%: A, 91%; B, 59%; C, 25%. Two patients died of an operation that permanently cured three patients in whom disease had spread beyond local confines. Abdominoperineal resection offered a 52% 5-year survival rate, increased the rate of cures in the unstaged patient by at least 5% over that afforded by local therapy, gave a 14% chance of cure in stage C disease compared with 0% with local therapy, and, compared with local therapy, was at least 67% more likely to offer cure than to kill in the event of disease spread to regional lymph nodes.", "PMID": 975012}
{"id": "pubmed23n0032_10096", "title": "The long-term course of carcinoma in situ of the uterine cervix.", "content": "The malignant potential of carcinoma in situ (CIS) of the uterine cervix has been the subject of great controversy. Despite refinements and additions to knowledge in this area, few reports on the long-term course of the disease have appeared in the past decade. Recent developments in diagnostic and therapeutic techniques coupled with changes in the patient population with this disease have prompted renewed interest in conservative management. Results of long-term observation of a group of patients followed initially without ablative therapy are reported. The data indicate that CIS of the uterine cervix is not the inevitably progressive disease that it has been considered to be. Unequivocal invasive cancer develops in only a small percentage of cases and can be controlled, if not cured, by current therapeutic modalities. The intraepithelial lesion, however, tends to persist despite conization, and eventually requires ablative therapy in most cases. Conservative procedures should be regarded as temporizing, at least until their long-term benefits can be recorded.", "contents": "The long-term course of carcinoma in situ of the uterine cervix. The malignant potential of carcinoma in situ (CIS) of the uterine cervix has been the subject of great controversy. Despite refinements and additions to knowledge in this area, few reports on the long-term course of the disease have appeared in the past decade. Recent developments in diagnostic and therapeutic techniques coupled with changes in the patient population with this disease have prompted renewed interest in conservative management. Results of long-term observation of a group of patients followed initially without ablative therapy are reported. The data indicate that CIS of the uterine cervix is not the inevitably progressive disease that it has been considered to be. Unequivocal invasive cancer develops in only a small percentage of cases and can be controlled, if not cured, by current therapeutic modalities. The intraepithelial lesion, however, tends to persist despite conization, and eventually requires ablative therapy in most cases. Conservative procedures should be regarded as temporizing, at least until their long-term benefits can be recorded.", "PMID": 975013}
{"id": "pubmed23n0032_10097", "title": "Normal pregnancy and delivery after cerebral metastases of choriocarcinoma: Case report.", "content": "Normal pregnancy and delivery after chemotherapy and radiation of cerebral metastases in a case of choriocarcinoma is presented. The need of surgical approach to these lesions as proposed by Stilp and his group is questioned.", "contents": "Normal pregnancy and delivery after cerebral metastases of choriocarcinoma: Case report. Normal pregnancy and delivery after chemotherapy and radiation of cerebral metastases in a case of choriocarcinoma is presented. The need of surgical approach to these lesions as proposed by Stilp and his group is questioned.", "PMID": 975014}
{"id": "pubmed23n0032_10098", "title": "Five-year remissions of metastatic solid teratoma of the ovary.", "content": "To date, only three 5-year survivors with metastatic solid malignant teratoma of the ovary have been reported. The authors report two additional cases surviving 5 years or greater. In both cases, the embryonal teratomatous elements matured to Grade 0 peritoneal metastases after intensive chemotherapy and surgery with or without radiotherapy. Since only one of the two patients had an objective response to therapy, we speculated that the maturation of the tumor in both patients might be, but is not proven to be, a consequence of chemotherapy, surgery, and/or radiotherapy. Because the prognosis, even in Stage IA solid ovarian teratoma, is so poor, we suggest that all such patients should have 2 years of adjuvant chemotherapy consisting of vincristine, dactinomycin, and cyclophosphamide. Patients with demonstrable metastases should receive the same chemotherapy until either all tumor has regressed or all immature elements have reverted to mature tissue.", "contents": "Five-year remissions of metastatic solid teratoma of the ovary. To date, only three 5-year survivors with metastatic solid malignant teratoma of the ovary have been reported. The authors report two additional cases surviving 5 years or greater. In both cases, the embryonal teratomatous elements matured to Grade 0 peritoneal metastases after intensive chemotherapy and surgery with or without radiotherapy. Since only one of the two patients had an objective response to therapy, we speculated that the maturation of the tumor in both patients might be, but is not proven to be, a consequence of chemotherapy, surgery, and/or radiotherapy. Because the prognosis, even in Stage IA solid ovarian teratoma, is so poor, we suggest that all such patients should have 2 years of adjuvant chemotherapy consisting of vincristine, dactinomycin, and cyclophosphamide. Patients with demonstrable metastases should receive the same chemotherapy until either all tumor has regressed or all immature elements have reverted to mature tissue.", "PMID": 975015}
{"id": "pubmed23n0032_10099", "title": "Dental management of patients irradiated for oral cancer.", "content": "Management of patients irradiated for oral cancer should include consideration of their oral health prior to, and after, radiation therapy. Data from 130 patients, followed for a period of 1 to 10 years, are presented and evaluated. The philosophy of retention and maintenance of as many teeth as possible is supported by this data. Extraction of teeth with severe periodontal disease after irradiation also proves to be a relatively safe operation. Osteoradionecrosis tends to be limited in extent and is generally well tolerated by the patient when treated conservatively. A treatment regimen is presented that significantly reduces the morbidity from therapeutic irradiation of the jaws. A comprehensive dental evaluation and follow-up plan coupled with patient cooperation are instrumental to the success of this program.", "contents": "Dental management of patients irradiated for oral cancer. Management of patients irradiated for oral cancer should include consideration of their oral health prior to, and after, radiation therapy. Data from 130 patients, followed for a period of 1 to 10 years, are presented and evaluated. The philosophy of retention and maintenance of as many teeth as possible is supported by this data. Extraction of teeth with severe periodontal disease after irradiation also proves to be a relatively safe operation. Osteoradionecrosis tends to be limited in extent and is generally well tolerated by the patient when treated conservatively. A treatment regimen is presented that significantly reduces the morbidity from therapeutic irradiation of the jaws. A comprehensive dental evaluation and follow-up plan coupled with patient cooperation are instrumental to the success of this program.", "PMID": 975016}
{"id": "pubmed23n0032_10100", "title": "Postulated developmental forms in the life cycle of the lymphocystis virus.", "content": "Electron micrographs of lymphocystis sk8n lesions obtained from the walleye pike, Stizostedion vitreum, show viral particles of differing sizes and morphologies. Earlier studies have described only particles ranging in size from 200-250 mmu in diameter. We show, in addition, new forms (65-100 mmu in diameter) intermingled among the large particles and also contained within the latter. Both forms are associated with a single inclusion body. The possible role of the small particle in the life cycle of the virus is discussed.", "contents": "Postulated developmental forms in the life cycle of the lymphocystis virus. Electron micrographs of lymphocystis sk8n lesions obtained from the walleye pike, Stizostedion vitreum, show viral particles of differing sizes and morphologies. Earlier studies have described only particles ranging in size from 200-250 mmu in diameter. We show, in addition, new forms (65-100 mmu in diameter) intermingled among the large particles and also contained within the latter. Both forms are associated with a single inclusion body. The possible role of the small particle in the life cycle of the virus is discussed.", "PMID": 975017}
{"id": "pubmed23n0032_10101", "title": "Comparison of the effects of nitrogen mustard on the functional properties of mitochondria from sensitive and resistant strains of Ehrlich ascites tumors.", "content": "In vivo treatment of sensitive tumor cells with nitrogen mustard (HN2) results in marked inhibitions of protein and nucleic acid synthesis by mitochondria subsequently isolated from these cells. This inhibition occurs at doses of drug which produce no apparent inhibition of total RNA synthesis. The inhibition gradually reverses itself in sensitive cells and is less severe and more rapidly reversed in resistant cells. The in vivo sensitivity of the mitochondria is in striking contrast to their in vitro behavior; isolated mitochondria resist 50 times the in vivo ID50 level of the drug. The sensitivity of protein and RNA synthesis in mitochondria is presumed to be related to the differences in organization between the nucleocytoplasmic processes and the mitochondrial. But the data also suggest that the in vivo effects on mitochondria are indirect, either acting via the cytoplasm or nucleus or via a carrier mechanism having mitochondrial affinity.", "contents": "Comparison of the effects of nitrogen mustard on the functional properties of mitochondria from sensitive and resistant strains of Ehrlich ascites tumors. In vivo treatment of sensitive tumor cells with nitrogen mustard (HN2) results in marked inhibitions of protein and nucleic acid synthesis by mitochondria subsequently isolated from these cells. This inhibition occurs at doses of drug which produce no apparent inhibition of total RNA synthesis. The inhibition gradually reverses itself in sensitive cells and is less severe and more rapidly reversed in resistant cells. The in vivo sensitivity of the mitochondria is in striking contrast to their in vitro behavior; isolated mitochondria resist 50 times the in vivo ID50 level of the drug. The sensitivity of protein and RNA synthesis in mitochondria is presumed to be related to the differences in organization between the nucleocytoplasmic processes and the mitochondrial. But the data also suggest that the in vivo effects on mitochondria are indirect, either acting via the cytoplasm or nucleus or via a carrier mechanism having mitochondrial affinity.", "PMID": 975018}
{"id": "pubmed23n0032_10102", "title": "Gangliosides and other lipids of hyperplastic liver nodules induced by N-2-fluorenylacetamide.", "content": "Hyperplastic liver nodules induced in rats fed the carcinogen N-2-fluorenylacetamide had levels of total, protein-bound, and ganglioside sialic acid above those of controls. Yet, the total lipid content of hyperplastic nodules and surrounding tissue was nearly identical to the lipid content of control liver. Hyperplastic nodules and surrounding liver did not differ from control tissue in phospholipid distribution patterns or in fatty acid composition of total lipids, neutral lipids, and/or polar lipids. Elevated levels of both free and esterified sterols, and a slightly lower level of phospholipid, were found in both hyperplastic nodules and in surrounding tissue as compared to control liver. The increased sterol ester content was largely due to increased amounts of cholesterol oleate. Thin-layer chromatography of ganglioside extracts revealed all gangliosides of control liver. Amounts of tri- and tetrasialoganglioside were reduced and levels of disialoganglioside were increased in modules relative to both control liver and surrounding tissue. The ganglioside alterations may represent at least one significant minimal deviation of hyperplastic nodules toward the malignant phenotype in keeping with the hypothesis that hyperplastic nodules represent a pre malignant form of hyperplasia.", "contents": "Gangliosides and other lipids of hyperplastic liver nodules induced by N-2-fluorenylacetamide. Hyperplastic liver nodules induced in rats fed the carcinogen N-2-fluorenylacetamide had levels of total, protein-bound, and ganglioside sialic acid above those of controls. Yet, the total lipid content of hyperplastic nodules and surrounding tissue was nearly identical to the lipid content of control liver. Hyperplastic nodules and surrounding liver did not differ from control tissue in phospholipid distribution patterns or in fatty acid composition of total lipids, neutral lipids, and/or polar lipids. Elevated levels of both free and esterified sterols, and a slightly lower level of phospholipid, were found in both hyperplastic nodules and in surrounding tissue as compared to control liver. The increased sterol ester content was largely due to increased amounts of cholesterol oleate. Thin-layer chromatography of ganglioside extracts revealed all gangliosides of control liver. Amounts of tri- and tetrasialoganglioside were reduced and levels of disialoganglioside were increased in modules relative to both control liver and surrounding tissue. The ganglioside alterations may represent at least one significant minimal deviation of hyperplastic nodules toward the malignant phenotype in keeping with the hypothesis that hyperplastic nodules represent a pre malignant form of hyperplasia.", "PMID": 975019}
{"id": "pubmed23n0032_10103", "title": "Combination chemotherapy in vitro with adriamycin. Observations of additive, antagonistic, and synergistic effects when used in two-drug combinations on cultured human lymphoma cells.", "content": "Adriamycin was paired with 12 other chemotherapeutic drugs in a search for possible synergistic pairs. Cell lethality was investigated by the colony-formation method utilizing a long-term human lymphoma cell line. Addative effects were noted with CCNU, ara-C, prednisolone, DDP, VP-16, and Yoshi, and mild synergistic effects with BLEO and CS. Adriamycin reduced the killing ability of BCNU and MeCCNU when applied simultaneously. This antagonistic effect was canceled by sequential incubation with each drug. It appears that no significant synergism can be expected by simultaneous pairing of adriamycin with some currently available chemotherapeutic agents.", "contents": "Combination chemotherapy in vitro with adriamycin. Observations of additive, antagonistic, and synergistic effects when used in two-drug combinations on cultured human lymphoma cells. Adriamycin was paired with 12 other chemotherapeutic drugs in a search for possible synergistic pairs. Cell lethality was investigated by the colony-formation method utilizing a long-term human lymphoma cell line. Addative effects were noted with CCNU, ara-C, prednisolone, DDP, VP-16, and Yoshi, and mild synergistic effects with BLEO and CS. Adriamycin reduced the killing ability of BCNU and MeCCNU when applied simultaneously. This antagonistic effect was canceled by sequential incubation with each drug. It appears that no significant synergism can be expected by simultaneous pairing of adriamycin with some currently available chemotherapeutic agents.", "PMID": 975020}
{"id": "pubmed23n0032_10104", "title": "Fab dimers of antitumor immunoglobulins as covalent carriers of daunomycin.", "content": "Fab dimers were prepared by pepsin digestion from the immunoglobulin fraction of a rabbit antiserum towards the Yac Moloney virus lymphoma cells. Daunomycin was attatched to these (Fab)2 by covalent binding. The resultant conjugates exerted pharmacological toxic activity and specificity towards Yac target cells similar to that observed previously with conjugates of intact anti-Yac IgG. The activity was manifested both in vitro by inhibition of RNA synthesis and by the effect of reduction of the growth of the tumor cells in vivo after short exposure to the conjugates. The potential advantage of using an IgG molecule devoid of its Fc portion is discussed.", "contents": "Fab dimers of antitumor immunoglobulins as covalent carriers of daunomycin. Fab dimers were prepared by pepsin digestion from the immunoglobulin fraction of a rabbit antiserum towards the Yac Moloney virus lymphoma cells. Daunomycin was attatched to these (Fab)2 by covalent binding. The resultant conjugates exerted pharmacological toxic activity and specificity towards Yac target cells similar to that observed previously with conjugates of intact anti-Yac IgG. The activity was manifested both in vitro by inhibition of RNA synthesis and by the effect of reduction of the growth of the tumor cells in vivo after short exposure to the conjugates. The potential advantage of using an IgG molecule devoid of its Fc portion is discussed.", "PMID": 975021}
{"id": "pubmed23n0032_10105", "title": "Preparation and properties of the O-methyl derivatives of 9-beta-D-arabinofuranosyladenine.", "content": "The previously reported resistance of 9-substituted adenines to ring alkylation in alkaline medium was profited from to prepare all seven possible O' methyl derivatives of the therapeutically important 9-beta-D-arabinofuranosyladenine (araA) by mild methylation of the latter with dimethylsulfate in aqueous alkaline medium. All the products were fractionated and isolated in a single step on a Dowex OH- column. The sequence of elution of the various derivatives was strikingly similar to that for the O' methyl derivatives of 1-beta-D-arabinofuranosylcytosine, previously reported, suggestive of a similar sequence of acidities of the sugar hydroxyls. The products were identified by various criteria, including PMR spectroscopy, extensive data for which are supplied. The 2'-O-methyl and 3'-O-methyl derivatives of araA exhibited appreciably lower susceptibility to calf intestinal adenosine deaminase than the parent araA. The 5'-O-methyl analogue was fully resistant to enzymatic deamination.", "contents": "Preparation and properties of the O-methyl derivatives of 9-beta-D-arabinofuranosyladenine. The previously reported resistance of 9-substituted adenines to ring alkylation in alkaline medium was profited from to prepare all seven possible O' methyl derivatives of the therapeutically important 9-beta-D-arabinofuranosyladenine (araA) by mild methylation of the latter with dimethylsulfate in aqueous alkaline medium. All the products were fractionated and isolated in a single step on a Dowex OH- column. The sequence of elution of the various derivatives was strikingly similar to that for the O' methyl derivatives of 1-beta-D-arabinofuranosylcytosine, previously reported, suggestive of a similar sequence of acidities of the sugar hydroxyls. The products were identified by various criteria, including PMR spectroscopy, extensive data for which are supplied. The 2'-O-methyl and 3'-O-methyl derivatives of araA exhibited appreciably lower susceptibility to calf intestinal adenosine deaminase than the parent araA. The 5'-O-methyl analogue was fully resistant to enzymatic deamination.", "PMID": 975022}
{"id": "pubmed23n0032_10106", "title": "Transfer RNA and aminoacyl tRNA synthetases in hormone dependent and independent mammary tumors of GR mice: I. Comparative study of the amino acid accepting capacity of the tRNA's in the presence of the homologous and heterologous enzymes.", "content": "Transfer RNA and aminoacyl tRNA synthetases were obtained from hormone dependent and independent mammary tumors of GR mice. We have studied the possible changes in the tRNA level by comparing the specific activities of the hormone dependent and independent mammary tumor tRNA's, but no differences were observed. Differences were found in the ability of enzymes from normal male GR mice liver, independent mammary tumor and dependent mammary tumor to cross-react with heterologous tRNA.", "contents": "Transfer RNA and aminoacyl tRNA synthetases in hormone dependent and independent mammary tumors of GR mice: I. Comparative study of the amino acid accepting capacity of the tRNA's in the presence of the homologous and heterologous enzymes. Transfer RNA and aminoacyl tRNA synthetases were obtained from hormone dependent and independent mammary tumors of GR mice. We have studied the possible changes in the tRNA level by comparing the specific activities of the hormone dependent and independent mammary tumor tRNA's, but no differences were observed. Differences were found in the ability of enzymes from normal male GR mice liver, independent mammary tumor and dependent mammary tumor to cross-react with heterologous tRNA.", "PMID": 975023}
{"id": "pubmed23n0032_10107", "title": "Quantification of two basic proteins related to a thymic hormone (LSH) and their occurrence in serum of normal and cancerous adults.", "content": "A quantitative assay for a thymic hormone (LSHr) was developed and utilized for biologic materials. The protein was isolated from tissue extracts or biologic fluids by precipitation with 20% (NH4)2SO4 and reprecipitation with 75% methanol with subsequent disc-electrophoresis on polyacrylamide gel. The proteins on the gel were stained with amido black. One of the two protein bands was readily identified with LSHr by its electrophoretic mobility and agar immunodiffusion tests. Comparison of the intensity of the protein bands obtained from biologic tissues with those of graded amounts of the pure LSHr allowed quantitative estimation. Another basic protein of the serum is found along with LSHr by the above fractionation procedure; it was tentatively named GEM 126 on the basis of its electrophoretic mobility. The total quantity of each of these serum basic proteins and their ratios to each other showed no correlation to any of 20 clinical and laboratory parameters in either healthy or cancerous adults. The lack of correlation between the quantity of serum protein presumed to be LSHr and cancer susceptibility suggests this thymic hormone is not deficient in patients with cancer. Treatment of female cancer patients with radiation and chemotherapy caused a decrease in serum GEM 126 when compared to healthy adults, and untreated or surgically treated cancer patients.", "contents": "Quantification of two basic proteins related to a thymic hormone (LSH) and their occurrence in serum of normal and cancerous adults. A quantitative assay for a thymic hormone (LSHr) was developed and utilized for biologic materials. The protein was isolated from tissue extracts or biologic fluids by precipitation with 20% (NH4)2SO4 and reprecipitation with 75% methanol with subsequent disc-electrophoresis on polyacrylamide gel. The proteins on the gel were stained with amido black. One of the two protein bands was readily identified with LSHr by its electrophoretic mobility and agar immunodiffusion tests. Comparison of the intensity of the protein bands obtained from biologic tissues with those of graded amounts of the pure LSHr allowed quantitative estimation. Another basic protein of the serum is found along with LSHr by the above fractionation procedure; it was tentatively named GEM 126 on the basis of its electrophoretic mobility. The total quantity of each of these serum basic proteins and their ratios to each other showed no correlation to any of 20 clinical and laboratory parameters in either healthy or cancerous adults. The lack of correlation between the quantity of serum protein presumed to be LSHr and cancer susceptibility suggests this thymic hormone is not deficient in patients with cancer. Treatment of female cancer patients with radiation and chemotherapy caused a decrease in serum GEM 126 when compared to healthy adults, and untreated or surgically treated cancer patients.", "PMID": 975024}
{"id": "pubmed23n0032_10108", "title": "In vivo studies on the mechanism of action of the tumor inhibitor vernolepin in the Walker 256 carcinosarcoma.", "content": "Rats bearing the Walker 256 intramuscular carcinosarcoma were treated intraperitoneally with tritium-labeled vernolepin or with its nontumor-inhibitory methanol adduct. Following treatment with 3H-vernolepin on several different dosage schedules, the tumors were found to contain significantly more radioactivity per gram wet weight than control tissue (muscle from the contralateral limb). After the administration of the nontumor-inhibitory methanol adduct, no such difference was observed. The distribution of radioactivity in various other organs (liver, kidney, spleen, intestine, lung, heat, fat, blood, and brain) was measured following treatment with the parent compound (3H-vernolepin). The implications of these data in terms of the suggested mechanism of action of sesquiterpene lactone tumor inhibitors is discussed.", "contents": "In vivo studies on the mechanism of action of the tumor inhibitor vernolepin in the Walker 256 carcinosarcoma. Rats bearing the Walker 256 intramuscular carcinosarcoma were treated intraperitoneally with tritium-labeled vernolepin or with its nontumor-inhibitory methanol adduct. Following treatment with 3H-vernolepin on several different dosage schedules, the tumors were found to contain significantly more radioactivity per gram wet weight than control tissue (muscle from the contralateral limb). After the administration of the nontumor-inhibitory methanol adduct, no such difference was observed. The distribution of radioactivity in various other organs (liver, kidney, spleen, intestine, lung, heat, fat, blood, and brain) was measured following treatment with the parent compound (3H-vernolepin). The implications of these data in terms of the suggested mechanism of action of sesquiterpene lactone tumor inhibitors is discussed.", "PMID": 975025}
{"id": "pubmed23n0032_10109", "title": "Acute effects of ethionine stereoisomers on hepatic RNA and protein synthesis in swiss mice.", "content": "The acute biochemical effects of ethionine have been well studied in rats but not in mice. These results show that both hepatic RNA and protein synthesis in Swiss mice were inhibited by DL-ethionine. Protein synthesis was inhibited 30 to 40 percent 3 hr after 2500 mg/kg DL-ethionine while RNA synthesis was inhibited 80 to 90 percent 3 hr after 625 mg/kg DL-ethionine. Thus ethionine was a far more potent inhibitor of RNA synthesis than of protein synthesis. There was no sex difference in either of these responses. While both stereoisomers were active, the L isomer was a more potent inhibitor of RNA synthesis than was the D isomer. In male mice, 3 hr after 20 mg/kg L-ethionine, RNA synthesis was inhibited 80%, while after D-ethionine it was inhibited only 51%.", "contents": "Acute effects of ethionine stereoisomers on hepatic RNA and protein synthesis in swiss mice. The acute biochemical effects of ethionine have been well studied in rats but not in mice. These results show that both hepatic RNA and protein synthesis in Swiss mice were inhibited by DL-ethionine. Protein synthesis was inhibited 30 to 40 percent 3 hr after 2500 mg/kg DL-ethionine while RNA synthesis was inhibited 80 to 90 percent 3 hr after 625 mg/kg DL-ethionine. Thus ethionine was a far more potent inhibitor of RNA synthesis than of protein synthesis. There was no sex difference in either of these responses. While both stereoisomers were active, the L isomer was a more potent inhibitor of RNA synthesis than was the D isomer. In male mice, 3 hr after 20 mg/kg L-ethionine, RNA synthesis was inhibited 80%, while after D-ethionine it was inhibited only 51%.", "PMID": 975026}
{"id": "pubmed23n0032_10110", "title": "Ribonuclease inhibitors from mouse ascites cells.", "content": "Ribonuclease inhibitors were found to be present attached to mouse ascites ribosomes and in the post-ribosomal supernatant. Both inhibitors inhibited pancreatic RNAses A and B and two endonucleases prepared from ascites cells but did not inhibit RNAses T1 or N1. Both inhibitors had the same sedimentation coefficient and this taken with the results above suggest that they are identical. The inhibitor was shown to interact directly with the RNAse itself.", "contents": "Ribonuclease inhibitors from mouse ascites cells. Ribonuclease inhibitors were found to be present attached to mouse ascites ribosomes and in the post-ribosomal supernatant. Both inhibitors inhibited pancreatic RNAses A and B and two endonucleases prepared from ascites cells but did not inhibit RNAses T1 or N1. Both inhibitors had the same sedimentation coefficient and this taken with the results above suggest that they are identical. The inhibitor was shown to interact directly with the RNAse itself.", "PMID": 975027}
{"id": "pubmed23n0032_10111", "title": "The resistance of putative premalignant liver cell populations, hyperplastic nodules, to the acute cytotoxic effects of some hepatocarcinogens.", "content": "The hypothesis that liver carcinogenesis may have as an important facet the early selection of carcinogen-resistant cells was tested in animals in which putative premalignant hepatocyte populations, hyperplastic nodules, were induced by 2-acetylaminofluorene or by ethionine. Hyperplastic nodules were observed to be resistant to the acute necrogenic effects of 2 hepatotoxins, CCl4 and dimethylnitrosamine, under conditions in which liver cell necrosis occurred in the liver surrounding the nodules. In addition, although [methyl-3H]dimethylnitrosamine was taken up to an equal degree in nodules and normal liver, the interactions with DNA, RNA, and protein in hyperplastic nodules were found to be about 50% less than in control liver. Hyperplastic nodules showed a marked decrease in uptake of [9-14C]-2-acetylaminofluorene, a finding that could account for the large decrease in labeling of DNA, RNA, and protein by [9-14C]-2acetylaminofluorene observed in the nodules. The results are consistent with and support the hypothesis that new hepatocyte populations that appear prior to cancer, during liver carcinogenesis, have as an important biological property a resistance to the cytotoxic effect of hepatocarcinogens. The basis for this resistance might be a decrease in uptake and/or a reduction in the level of activation of carcinogens.", "contents": "The resistance of putative premalignant liver cell populations, hyperplastic nodules, to the acute cytotoxic effects of some hepatocarcinogens. The hypothesis that liver carcinogenesis may have as an important facet the early selection of carcinogen-resistant cells was tested in animals in which putative premalignant hepatocyte populations, hyperplastic nodules, were induced by 2-acetylaminofluorene or by ethionine. Hyperplastic nodules were observed to be resistant to the acute necrogenic effects of 2 hepatotoxins, CCl4 and dimethylnitrosamine, under conditions in which liver cell necrosis occurred in the liver surrounding the nodules. In addition, although [methyl-3H]dimethylnitrosamine was taken up to an equal degree in nodules and normal liver, the interactions with DNA, RNA, and protein in hyperplastic nodules were found to be about 50% less than in control liver. Hyperplastic nodules showed a marked decrease in uptake of [9-14C]-2-acetylaminofluorene, a finding that could account for the large decrease in labeling of DNA, RNA, and protein by [9-14C]-2acetylaminofluorene observed in the nodules. The results are consistent with and support the hypothesis that new hepatocyte populations that appear prior to cancer, during liver carcinogenesis, have as an important biological property a resistance to the cytotoxic effect of hepatocarcinogens. The basis for this resistance might be a decrease in uptake and/or a reduction in the level of activation of carcinogens.", "PMID": 975037}
{"id": "pubmed23n0032_10112", "title": "Carbohydrate compositions of normal, spontaneously transformed, and virally transformed cells derived from BALB/c mice.", "content": "Carbohydrate compositions of chloroform: methanol-soluble and -insoluble complex polysaccharides have been studied in cell lines derived from BALB/c mice. The cells used in these studies include normal cells, spontaneous and viral transformants that cause tumors that regress, and spontaneous and viral transformants that cause progressively growing tumors that kill immunocompetent BALB/c mice. The carbohydrates were determined by gas-liquid chromatography. Some of the transformed cell lines compared with normal cells have altered carbohydrate compositions, including decreased sialic acid levels and decreased N-acetylgalactosamine in chloroform: methanol-soluble material. Significant decreases in total carbohydrates of chloroform: methanol-soluble material were also observed in some of the transformed cells. However, these changes alone do not predict cancer. Transformed cell lines that cause progressively growing tumors tend to have fewer alterations in carbohydrates of complex polysaccharides than lines causing tumors that regress.", "contents": "Carbohydrate compositions of normal, spontaneously transformed, and virally transformed cells derived from BALB/c mice. Carbohydrate compositions of chloroform: methanol-soluble and -insoluble complex polysaccharides have been studied in cell lines derived from BALB/c mice. The cells used in these studies include normal cells, spontaneous and viral transformants that cause tumors that regress, and spontaneous and viral transformants that cause progressively growing tumors that kill immunocompetent BALB/c mice. The carbohydrates were determined by gas-liquid chromatography. Some of the transformed cell lines compared with normal cells have altered carbohydrate compositions, including decreased sialic acid levels and decreased N-acetylgalactosamine in chloroform: methanol-soluble material. Significant decreases in total carbohydrates of chloroform: methanol-soluble material were also observed in some of the transformed cells. However, these changes alone do not predict cancer. Transformed cell lines that cause progressively growing tumors tend to have fewer alterations in carbohydrates of complex polysaccharides than lines causing tumors that regress.", "PMID": 975038}
{"id": "pubmed23n0032_10113", "title": "Combination versus sequential five-drug chemotherapy in metastatic carcinoma of the breast.", "content": "Patients with metastatic or recurrent adenocarcinoma of the breast were randomized to weekly combination chemotherapy, intermittent combination chemotherapy, or single-drug chemotherapy administered sequentially. Patients receiving weekly combination therapy were more likely to respond than those receiving single-drug therapy (5-fluorouracil). The median survival for either group treated with combination therapy was double that of patients on sequential therapy. One-fourth of the patients had a prolonged survival (greater than 75 weeks), regardless of therapy.", "contents": "Combination versus sequential five-drug chemotherapy in metastatic carcinoma of the breast. Patients with metastatic or recurrent adenocarcinoma of the breast were randomized to weekly combination chemotherapy, intermittent combination chemotherapy, or single-drug chemotherapy administered sequentially. Patients receiving weekly combination therapy were more likely to respond than those receiving single-drug therapy (5-fluorouracil). The median survival for either group treated with combination therapy was double that of patients on sequential therapy. One-fourth of the patients had a prolonged survival (greater than 75 weeks), regardless of therapy.", "PMID": 975039}
{"id": "pubmed23n0032_10114", "title": "Metabolism and metabolic effects of 8-azainosine and 8-azaadenosine.", "content": "8-Azainosine (8-aza-HR) is of interest because of its activity against experimental tumors. Metabolic studies in cell cultures were performed with 8-aza-HR and with the structurally related nucleoside, 8-azaadenosine (9-beta-D-ribofuranosyl-8-azaadenine) (8-aza-AR), which has a lower degree of antitumor activity than does 8-aza-HR. In H. Ep. 2 cells and in Ca755 cells, both 14C-labeled nucleosides were metabolized to nucleotides of 8-azaadenine (8-aza-A) and 8-azaguanine (8-aza-G) and incorporated into polynucleotides as 8-aza-A and 8-aza-G. 8-Aza HR was incorporated primarily as 8-aza-G, whereas 8-aza-AR was incorporated about equally as 8-aza-A and 8-aza-G. In H. Ep. 2 cells, the extent of incorporation of 8-aza-HR as 8-aza-G was about one-half that found when [14C]-8-aza-G was the precursor. In the H. Ep. 2/FA/FAR cell line, 8-aza-AR and 8-aza-HR were metabolized similarly, in that both were incorporated into polynucleotides principally as 8-aza-G; apparently, in this cell line which is deficient in adenosine kinase and adenine phosphoribosyltransferase, 8-aza-AR is metabolized by conversion to 8-aza-HR. A cell line (H. Ep 2/8-aza HR), which was resistant to 8-aza-HR but sensitive to 8-aza-AR and which retained hypoxanthine (guanine)-phosphoribosyltransferase activity, metabolized 8-aza-HR to only a small extent. However, in this cell-line, 8-aza-AR was more extensively metabolized and was incorporated primarily as 8-aza-A. The failure of these cells to convert 8-aza-AR or 8-aza-HR to 8-aza-G indicates that the basis for resistance may be a change in the substrate specificities of the enzymes of guanosine monophosphate synthesis such that these cells no longer effectively convert 8-azainosine monophosphate to 8-azaguanosine monophosphate. 8-Aza-AR was a potent inhibitor of purine synthesis de novo, but 8-aza-HR, at concentrations much higher than the inhibitory concentration of 8-aza-AR, did not inhibit this process. In H. Ep. 2 cells, 8-aza-HR blocked the conversion of orotic acid to uridine nucleotides and caused an accumulation of orotidine. This inhibition of pyrimidine biosynthesis apparently does not contribute significantly to the cytotoxicity of 8-aza-HR because uridine provided no degree of reversal of its inhibition of the growth of cell cultures.", "contents": "Metabolism and metabolic effects of 8-azainosine and 8-azaadenosine. 8-Azainosine (8-aza-HR) is of interest because of its activity against experimental tumors. Metabolic studies in cell cultures were performed with 8-aza-HR and with the structurally related nucleoside, 8-azaadenosine (9-beta-D-ribofuranosyl-8-azaadenine) (8-aza-AR), which has a lower degree of antitumor activity than does 8-aza-HR. In H. Ep. 2 cells and in Ca755 cells, both 14C-labeled nucleosides were metabolized to nucleotides of 8-azaadenine (8-aza-A) and 8-azaguanine (8-aza-G) and incorporated into polynucleotides as 8-aza-A and 8-aza-G. 8-Aza HR was incorporated primarily as 8-aza-G, whereas 8-aza-AR was incorporated about equally as 8-aza-A and 8-aza-G. In H. Ep. 2 cells, the extent of incorporation of 8-aza-HR as 8-aza-G was about one-half that found when [14C]-8-aza-G was the precursor. In the H. Ep. 2/FA/FAR cell line, 8-aza-AR and 8-aza-HR were metabolized similarly, in that both were incorporated into polynucleotides principally as 8-aza-G; apparently, in this cell line which is deficient in adenosine kinase and adenine phosphoribosyltransferase, 8-aza-AR is metabolized by conversion to 8-aza-HR. A cell line (H. Ep 2/8-aza HR), which was resistant to 8-aza-HR but sensitive to 8-aza-AR and which retained hypoxanthine (guanine)-phosphoribosyltransferase activity, metabolized 8-aza-HR to only a small extent. However, in this cell-line, 8-aza-AR was more extensively metabolized and was incorporated primarily as 8-aza-A. The failure of these cells to convert 8-aza-AR or 8-aza-HR to 8-aza-G indicates that the basis for resistance may be a change in the substrate specificities of the enzymes of guanosine monophosphate synthesis such that these cells no longer effectively convert 8-azainosine monophosphate to 8-azaguanosine monophosphate. 8-Aza-AR was a potent inhibitor of purine synthesis de novo, but 8-aza-HR, at concentrations much higher than the inhibitory concentration of 8-aza-AR, did not inhibit this process. In H. Ep. 2 cells, 8-aza-HR blocked the conversion of orotic acid to uridine nucleotides and caused an accumulation of orotidine. This inhibition of pyrimidine biosynthesis apparently does not contribute significantly to the cytotoxicity of 8-aza-HR because uridine provided no degree of reversal of its inhibition of the growth of cell cultures.", "PMID": 975040}
{"id": "pubmed23n0032_10115", "title": "Malignant transformation and viral replication of rat bone and muscle cells after in vitro infection with rat-adapted murine sarcoma virus (Moloney).", "content": "Isolated bone and muscle cells from rat fetuses were infected with the rat-adapted osteosarcomogenic murine sarcoma virus (Moloney) for examination of malignant transformation and viral replication. After the infection, the bone cells underwent an unusual transformation characterized by two patients in the focus formation. In the early transformation (Transformation I), foci consisting of only morphologically altered cells appeared, but they soon disappeared. Focus formation of this type continued for subsequent cell passages. In the late transformation (Transformation II), typical foci of malignant cells were formed with rapid multiplication. The cells in both types of transformation produced sarcomogenic as well as leukemogenic viruses. They contained C-type particles showing varying morphology and dimensions. Distinct bone cell tropism of osteosarcomogenic murine sarcoma virus (Moloney) was shown for three in vitro passages through rat bone cells. The infected muscles cells produced Transformation I foci at an incidence lower than did the bone cells, being mostly nontransformed. The virus yielded from these cells was predominantly leukemogenic, consisting of typical C-type particles. Evidence presented suggests that the tissue dependency with replicating ability of murine sarcoma virus and murine leukemia virus present in osteosarcomogenic murine sarcoma virus (Moloney) preparation is responsible for exhibiting the tissue tropism of this virus.", "contents": "Malignant transformation and viral replication of rat bone and muscle cells after in vitro infection with rat-adapted murine sarcoma virus (Moloney). Isolated bone and muscle cells from rat fetuses were infected with the rat-adapted osteosarcomogenic murine sarcoma virus (Moloney) for examination of malignant transformation and viral replication. After the infection, the bone cells underwent an unusual transformation characterized by two patients in the focus formation. In the early transformation (Transformation I), foci consisting of only morphologically altered cells appeared, but they soon disappeared. Focus formation of this type continued for subsequent cell passages. In the late transformation (Transformation II), typical foci of malignant cells were formed with rapid multiplication. The cells in both types of transformation produced sarcomogenic as well as leukemogenic viruses. They contained C-type particles showing varying morphology and dimensions. Distinct bone cell tropism of osteosarcomogenic murine sarcoma virus (Moloney) was shown for three in vitro passages through rat bone cells. The infected muscles cells produced Transformation I foci at an incidence lower than did the bone cells, being mostly nontransformed. The virus yielded from these cells was predominantly leukemogenic, consisting of typical C-type particles. Evidence presented suggests that the tissue dependency with replicating ability of murine sarcoma virus and murine leukemia virus present in osteosarcomogenic murine sarcoma virus (Moloney) preparation is responsible for exhibiting the tissue tropism of this virus.", "PMID": 975041}
{"id": "pubmed23n0032_10116", "title": "Selective lethal effect of supranormal temperatures on human neoplastic cells.", "content": "The effects of supranormal temperatures upon normal human cells (derived from normal adult and embryonal tissues) and neoplastic human cells (derived from biopsies of malignant tumors) have been quantitatively studied in tissue culture. Melanoma cells have been compared with melanocytes derived from fetal uveas. Colon carcinoma cells have been compared with embryonal intestinal epithelial cells. Malignant neuroepithelial cells derived from a teratocarcinoma of the ovary have been compared with neuroepithelial cells derived from fetal brain. Fibrosarcoma cells have been compared with normal adult fibroblasts. All cells defined as neoplastic have produced malignant tumors when injected into nude thymus-deficient mice at doses of 1 X 10(7) cells or less. Exposure to temperatures of 42.5-43.0 degrees for 4 to 8 hr has been shown to have significantly greater lethal effect on the tumor cells than on the nonneoplastic cells.", "contents": "Selective lethal effect of supranormal temperatures on human neoplastic cells. The effects of supranormal temperatures upon normal human cells (derived from normal adult and embryonal tissues) and neoplastic human cells (derived from biopsies of malignant tumors) have been quantitatively studied in tissue culture. Melanoma cells have been compared with melanocytes derived from fetal uveas. Colon carcinoma cells have been compared with embryonal intestinal epithelial cells. Malignant neuroepithelial cells derived from a teratocarcinoma of the ovary have been compared with neuroepithelial cells derived from fetal brain. Fibrosarcoma cells have been compared with normal adult fibroblasts. All cells defined as neoplastic have produced malignant tumors when injected into nude thymus-deficient mice at doses of 1 X 10(7) cells or less. Exposure to temperatures of 42.5-43.0 degrees for 4 to 8 hr has been shown to have significantly greater lethal effect on the tumor cells than on the nonneoplastic cells.", "PMID": 975042}
{"id": "pubmed23n0032_10117", "title": "Molecular structure of benzo(a)pyrene 4,5-oxide.", "content": "An X-ray crystallographic study of benzo(a)pyrene 4,5-oxide, a metabolite of the carcinogen benzo(a)pyrene (BP), as given information on the geometry of this molecule. The carbon skeleton of BP itself has been shown by others to be early planar; the planarity of the carbon skeleton has been shown by this work to be perturbed very little by epoxidation of the 4,5-double bond. Epoxidation has, however, increased the double bond character of C-11--C-12, C-9--C-10, and C-7--C-8. The hydrogen atom on C-3 points directly toward the oxygen atom of another molecule. This C--H... O interaction, although weak, suggests that C-3 might be slightly acidic. An analysis of the experimentally determined bond lengths indicates that, after the highly reactive epoxide ring, the most reactive positions are at C-1, C-6, C-7, C-11, and C-12. The oxide ring of BP, unlike that for the K-region oxide of 7,12-dimethylbenz(a)anthracene, is symmetrical (with C--O distances equivalent within experimental error). The C--O distances are longer than those found in most oxides, including those in 7,12-dimethylbenz(a)anthracene-5,6-oxide. Thus it has been shown that the oxide rings of the K-region oxides of the two potent carcinogens BP and 7,12-dimethylbenz(a)anthracene are not similar in dimensions.", "contents": "Molecular structure of benzo(a)pyrene 4,5-oxide. An X-ray crystallographic study of benzo(a)pyrene 4,5-oxide, a metabolite of the carcinogen benzo(a)pyrene (BP), as given information on the geometry of this molecule. The carbon skeleton of BP itself has been shown by others to be early planar; the planarity of the carbon skeleton has been shown by this work to be perturbed very little by epoxidation of the 4,5-double bond. Epoxidation has, however, increased the double bond character of C-11--C-12, C-9--C-10, and C-7--C-8. The hydrogen atom on C-3 points directly toward the oxygen atom of another molecule. This C--H... O interaction, although weak, suggests that C-3 might be slightly acidic. An analysis of the experimentally determined bond lengths indicates that, after the highly reactive epoxide ring, the most reactive positions are at C-1, C-6, C-7, C-11, and C-12. The oxide ring of BP, unlike that for the K-region oxide of 7,12-dimethylbenz(a)anthracene, is symmetrical (with C--O distances equivalent within experimental error). The C--O distances are longer than those found in most oxides, including those in 7,12-dimethylbenz(a)anthracene-5,6-oxide. Thus it has been shown that the oxide rings of the K-region oxides of the two potent carcinogens BP and 7,12-dimethylbenz(a)anthracene are not similar in dimensions.", "PMID": 975043}
{"id": "pubmed23n0032_10118", "title": "Histogenetic and morphological in vivo and in vitro data concerning androgen-estrogen-induced scent gland tumor in the Syrian hamster.", "content": "The scent gland tumor of the Syrian hamster is induced by exogenous androgen and estrogen. Microscopic nodules are induced normally in old males by endogenous androgen. The histogenesis of the scent gland tumor is complex and not completely understood. In this study microscopic preneoplastic nodules and macroscopic tumors were studied by light and electron microscopy, and the macroscopic tumors were grown in tissue culture on collagen-coated coverslips and on sponge foam matrices by the organ culture method. The cultures were fed with an unfiltered fetal calf serum-bovine serum ultrafiltrate medium, which contained endogenous androgen-estrogen, 110-100 pg and could maintain growth without additional androgen-estrogen. Exogenous androgen-estrogen was also added to some cultures. Scent gland tumors grown in organ culture contained cells of two shapes, spindle and ovoid arranged in cords. Cultures on coverslips showed radiating outgrowths of spindle cells suggesting either mesenchymal or Schwann cells. By electron microscopy, both in vivo and in vitro preneoplastic and tumor samples contained cells with segments of basal lamina, micropinocytotic vesicles, and junctional complexes. These features were similar to those of poorly differentiated experimental malignant rat schwannomas maintained in similar in vitro systems. Tumors grown in vivo and in vitro were associated with collagen fibrils with a periodicity ranging from 400 to 1075 A. The evidence reported in this paper suggests that one component of the scent gland tumor is an androgen-estrogen-induced poorly differentiated schwannoma.", "contents": "Histogenetic and morphological in vivo and in vitro data concerning androgen-estrogen-induced scent gland tumor in the Syrian hamster. The scent gland tumor of the Syrian hamster is induced by exogenous androgen and estrogen. Microscopic nodules are induced normally in old males by endogenous androgen. The histogenesis of the scent gland tumor is complex and not completely understood. In this study microscopic preneoplastic nodules and macroscopic tumors were studied by light and electron microscopy, and the macroscopic tumors were grown in tissue culture on collagen-coated coverslips and on sponge foam matrices by the organ culture method. The cultures were fed with an unfiltered fetal calf serum-bovine serum ultrafiltrate medium, which contained endogenous androgen-estrogen, 110-100 pg and could maintain growth without additional androgen-estrogen. Exogenous androgen-estrogen was also added to some cultures. Scent gland tumors grown in organ culture contained cells of two shapes, spindle and ovoid arranged in cords. Cultures on coverslips showed radiating outgrowths of spindle cells suggesting either mesenchymal or Schwann cells. By electron microscopy, both in vivo and in vitro preneoplastic and tumor samples contained cells with segments of basal lamina, micropinocytotic vesicles, and junctional complexes. These features were similar to those of poorly differentiated experimental malignant rat schwannomas maintained in similar in vitro systems. Tumors grown in vivo and in vitro were associated with collagen fibrils with a periodicity ranging from 400 to 1075 A. The evidence reported in this paper suggests that one component of the scent gland tumor is an androgen-estrogen-induced poorly differentiated schwannoma.", "PMID": 975044}
{"id": "pubmed23n0032_10119", "title": "Carcinogenic activity of a chlorinated polyether polyurethan.", "content": "On the basis of the results of an earlier study, a particular polyurethan sample (Y-238) was selected for further evaluation of its carcinogenic potential. This sample was subjected to physical and chemical tests for elucidation of its chemical structure, molecular weight, and molecular weight distribution. Additional biological tests were conducted on male NBR rats by implanting various quantities of the sample i.p., while others received an intrabronchus implant. Tumors, assessed histologically as malignant, were observed following both routes of implantation. The most common neoplasms of the pulmonary site was epidermoid carcinoma, while fibrosarcoma was the most common neoplasm in the peritoneal cavity. Data from the i.p. implantation suggested a dose-related incidence of cancers.", "contents": "Carcinogenic activity of a chlorinated polyether polyurethan. On the basis of the results of an earlier study, a particular polyurethan sample (Y-238) was selected for further evaluation of its carcinogenic potential. This sample was subjected to physical and chemical tests for elucidation of its chemical structure, molecular weight, and molecular weight distribution. Additional biological tests were conducted on male NBR rats by implanting various quantities of the sample i.p., while others received an intrabronchus implant. Tumors, assessed histologically as malignant, were observed following both routes of implantation. The most common neoplasms of the pulmonary site was epidermoid carcinoma, while fibrosarcoma was the most common neoplasm in the peritoneal cavity. Data from the i.p. implantation suggested a dose-related incidence of cancers.", "PMID": 975045}
{"id": "pubmed23n0032_10120", "title": "Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice.", "content": "A human colon carcinoma cell line, Co-115, has been established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grow in vitro as tightly packed, epithelial-like colonies, have a doubling time of about 36 hr, have a relatively low plating efficiency in agar, and release significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature has been confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable of that of the original xenograft.", "contents": "Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice. A human colon carcinoma cell line, Co-115, has been established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grow in vitro as tightly packed, epithelial-like colonies, have a doubling time of about 36 hr, have a relatively low plating efficiency in agar, and release significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature has been confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable of that of the original xenograft.", "PMID": 975046}
{"id": "pubmed23n0032_10121", "title": "A factor responsible for increases in ornithine decarboxylase activity in the livers of tumor-bearing mice.", "content": "Ornithine decarboxylase activity in the livers, spleens, and kidneys of tumor-bearing mice changed markedly during tumor growth. These changes in enzyme activity were not due to infiltration or metastasis of tumor cells in these organs. After i.p. inoculation of Ehrlich tumor cells, enzyme activity in the liver and spleen increased remarkedly, reaching a peak in 4 to 6 days and then quickly decreasing. Conversely, activity in the kidney, which was very high in normal mice, decreased markedly during tumor growth, nearly reaching zero on Day 6 and remaining very low until death. Upon injection of a cell-free homogenate of Ehrlich tumor or cell-free ascites fluid, enzyme activity in the liver and spleen also increased markedly, but that in the kidney did not change. These increases in activity were not due to the effects of living tumor cells. Similar increases in enzyme activity were also observed in the livers of mice given injections of homogenates of Sarcoma 180 or Act. S tumor, or plasma from tumor-bearing mice, but not in the livers of mice given injections of homogenates of various nontumorous tissues, such as liver, kidney, spleen, muscle, regenerating liver, and fetus, or plasma obtained from normal mice. A similar increase in enzyme activity in the liver after injection of a cell-free preparation of tumor cells was observed in hypophysectomized and adrenalectomized mice; thus, these endocrine systems are probably not involved in the increase in enzyme activity in the livers of tumor-bearing mice.", "contents": "A factor responsible for increases in ornithine decarboxylase activity in the livers of tumor-bearing mice. Ornithine decarboxylase activity in the livers, spleens, and kidneys of tumor-bearing mice changed markedly during tumor growth. These changes in enzyme activity were not due to infiltration or metastasis of tumor cells in these organs. After i.p. inoculation of Ehrlich tumor cells, enzyme activity in the liver and spleen increased remarkedly, reaching a peak in 4 to 6 days and then quickly decreasing. Conversely, activity in the kidney, which was very high in normal mice, decreased markedly during tumor growth, nearly reaching zero on Day 6 and remaining very low until death. Upon injection of a cell-free homogenate of Ehrlich tumor or cell-free ascites fluid, enzyme activity in the liver and spleen also increased markedly, but that in the kidney did not change. These increases in activity were not due to the effects of living tumor cells. Similar increases in enzyme activity were also observed in the livers of mice given injections of homogenates of Sarcoma 180 or Act. S tumor, or plasma from tumor-bearing mice, but not in the livers of mice given injections of homogenates of various nontumorous tissues, such as liver, kidney, spleen, muscle, regenerating liver, and fetus, or plasma obtained from normal mice. A similar increase in enzyme activity in the liver after injection of a cell-free preparation of tumor cells was observed in hypophysectomized and adrenalectomized mice; thus, these endocrine systems are probably not involved in the increase in enzyme activity in the livers of tumor-bearing mice.", "PMID": 975047}
{"id": "pubmed23n0032_10122", "title": "Stimulated DNA synthesis in mouse epidermal cell cultures treated with 12-O-tetradecanoyl-phorbol-13-acetate.", "content": "Exposure of mouse epidermal cells in culture to 12-O-tetradecanoyl-phorobol-13-acetate (TPA) results in an initial inhibition of DNA synthesis for 24 hr followed by a 5- to 10-fold stimulation at 72 to 96 hr. A corresponding increase in mitotic rate also occurs at 72 to 96 hr. These responses occur when TPA is continuously present in the medium or if the exposure is as short as 1 hr, but the degree of stimulation was dependent on dose and duration of exposure. Sensitivity to TPA varied with the length of time the cells were in culture prior to treatment. TPA treatment also produced an alteration in morphology from clearly epithelial to a more fibroblastic type. These biochemical and morphological effects did not occur after treatment of epidermal cells with either phorbol-13,20-diacetate or phorbol. Primary dermal fibroblasts in culture did not respond to TPA in this manner, but a line of cultured liver epithelial cells was slightly stimulated by the promoter. This system appears to be a sensitive in vitro model for detecting the hyperplasia-inducing effects of phorbol esters and should be used for mechanistic studies and bioassay.", "contents": "Stimulated DNA synthesis in mouse epidermal cell cultures treated with 12-O-tetradecanoyl-phorbol-13-acetate. Exposure of mouse epidermal cells in culture to 12-O-tetradecanoyl-phorobol-13-acetate (TPA) results in an initial inhibition of DNA synthesis for 24 hr followed by a 5- to 10-fold stimulation at 72 to 96 hr. A corresponding increase in mitotic rate also occurs at 72 to 96 hr. These responses occur when TPA is continuously present in the medium or if the exposure is as short as 1 hr, but the degree of stimulation was dependent on dose and duration of exposure. Sensitivity to TPA varied with the length of time the cells were in culture prior to treatment. TPA treatment also produced an alteration in morphology from clearly epithelial to a more fibroblastic type. These biochemical and morphological effects did not occur after treatment of epidermal cells with either phorbol-13,20-diacetate or phorbol. Primary dermal fibroblasts in culture did not respond to TPA in this manner, but a line of cultured liver epithelial cells was slightly stimulated by the promoter. This system appears to be a sensitive in vitro model for detecting the hyperplasia-inducing effects of phorbol esters and should be used for mechanistic studies and bioassay.", "PMID": 975048}
{"id": "pubmed23n0032_10123", "title": "Potentiation of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea by amphotericin B in murine ependymoblastoma.", "content": "This paper reports the potentiation of the therapeutic effect of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) by amphotericin B (AMB) in s.c. transplanted murine ependymoblastoma 01B111. The rate of 2-month cures was 6% when tumors were treated 10 to 12 days after transplantation by a single 6 i.m. injection of 2.5 mg of CCNU per kg and reached 15% with 10 mg of CCNU per kg. When 25 mg of AMB per kg were given i.p. 10 hr prior to CCNU, the respective figures increased to 18 and 58%, the differences being significant at 5 and 1%. A single dose of 25 mg of AMB per kg given alone did not affect tumor growth. Radioactivity per g of tumor was measured 30, 60, and 120 min after injection of [14C]CCNU in total tissue, chloroform:methanol (2:1, v/v) extract, and CCNU isolated by thin-layer chromatography. No difference was found between animals treated with 25 mg of AMB per kg and controls. The inhibition of DNA synthesis, measured 24 hr after the administration of CCNU and 2 hr after the injection of [3H]thymidine, was almost optimal with 5 mg of CCNU per kg. The inhibition caused by 1 mg of CCNU per kg was not enhanced by AMB. Thus, the potentiation of CCNU by AMB does not seem attributable to an increased permeability of the tumor to CCNU or to an enhancement of the inhibition of DNA synthesis, at least in murine ependymoblastoma.", "contents": "Potentiation of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea by amphotericin B in murine ependymoblastoma. This paper reports the potentiation of the therapeutic effect of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) by amphotericin B (AMB) in s.c. transplanted murine ependymoblastoma 01B111. The rate of 2-month cures was 6% when tumors were treated 10 to 12 days after transplantation by a single 6 i.m. injection of 2.5 mg of CCNU per kg and reached 15% with 10 mg of CCNU per kg. When 25 mg of AMB per kg were given i.p. 10 hr prior to CCNU, the respective figures increased to 18 and 58%, the differences being significant at 5 and 1%. A single dose of 25 mg of AMB per kg given alone did not affect tumor growth. Radioactivity per g of tumor was measured 30, 60, and 120 min after injection of [14C]CCNU in total tissue, chloroform:methanol (2:1, v/v) extract, and CCNU isolated by thin-layer chromatography. No difference was found between animals treated with 25 mg of AMB per kg and controls. The inhibition of DNA synthesis, measured 24 hr after the administration of CCNU and 2 hr after the injection of [3H]thymidine, was almost optimal with 5 mg of CCNU per kg. The inhibition caused by 1 mg of CCNU per kg was not enhanced by AMB. Thus, the potentiation of CCNU by AMB does not seem attributable to an increased permeability of the tumor to CCNU or to an enhancement of the inhibition of DNA synthesis, at least in murine ependymoblastoma.", "PMID": 975049}
{"id": "pubmed23n0032_10124", "title": "Comparison of antitumor activities of pancreatic ribonuclease and its cross-linked dimer.", "content": "The cross-linked dimer of bovine pancreatic RNase (M.W. 28,000) is significantly more effective than the monomer in inhibiting tumor development in mice when administered i.p. 1 day after inoculation with sarcoma 180J ascites cells. Animals bearing solid tumors were not affected. In AKR/J mice with advanced leukemia, a single i.p. injection of 100 mug of the dimer led to about 50% reduction in the enlarged lymph nodes and the spleen at 24 hr. The half-life of the dimer in the bloodstream has been determined to be 10 min in rats and 6 min in mice, compared to values of 5 and 3.5 min, respectively, for the monomer. Analyses of the tissues of untreated leukemic mice for RNase and RNase inhibitors show that the tumor tissues are not deficient in RNase activity. Considerations of possible mechanisms of action of the dimer indicate that other basic proteins in this size range may merit examination as cytostatic agents toward transformed cells.", "contents": "Comparison of antitumor activities of pancreatic ribonuclease and its cross-linked dimer. The cross-linked dimer of bovine pancreatic RNase (M.W. 28,000) is significantly more effective than the monomer in inhibiting tumor development in mice when administered i.p. 1 day after inoculation with sarcoma 180J ascites cells. Animals bearing solid tumors were not affected. In AKR/J mice with advanced leukemia, a single i.p. injection of 100 mug of the dimer led to about 50% reduction in the enlarged lymph nodes and the spleen at 24 hr. The half-life of the dimer in the bloodstream has been determined to be 10 min in rats and 6 min in mice, compared to values of 5 and 3.5 min, respectively, for the monomer. Analyses of the tissues of untreated leukemic mice for RNase and RNase inhibitors show that the tumor tissues are not deficient in RNase activity. Considerations of possible mechanisms of action of the dimer indicate that other basic proteins in this size range may merit examination as cytostatic agents toward transformed cells.", "PMID": 975050}
{"id": "pubmed23n0032_10125", "title": "Prolactin levels in populations at risk for breast cancer.", "content": "The basal plasma concentration of prolactin was determined by radioimmunoassay in Caucasian women at different risk for breast cancer and in three cases of women at risk for breast cancer. Samples taken under comparable conditions showed similar basal levels at birth and in prepubertal Bantu and Caucasian girls, or in healthy pre- and postmenopausal Bantu, Caucasian, or Japanese women. Elevated plasma prolactin levels were found in women castrated prior to 35 years of age and in women whose first pregnancy occured after 35 years. Evidence indicates that the prolactin/estrogen relationship may not be similar in women 35 to 45 years of age as compared to young women and suggests that elevation of serum prolactin per se does not appear to be related to an increased risk of breast cancer. The prolactin level was increased only in Caucasian women with breast cancer. If a high prolactin/estradiol ratio increases the susceptibility of the mammary epithelium to neoplastic growth, the lack of changes in prolactin levels in premenopausal Japanese patients and in postmenopausal patients of the three ethnic groups indicates that other factors are involved. Further study of the effects of life-style and diet on the basal level and stimulated release of prolactin is required to resolve the relationship of prolactin to breast cancer.", "contents": "Prolactin levels in populations at risk for breast cancer. The basal plasma concentration of prolactin was determined by radioimmunoassay in Caucasian women at different risk for breast cancer and in three cases of women at risk for breast cancer. Samples taken under comparable conditions showed similar basal levels at birth and in prepubertal Bantu and Caucasian girls, or in healthy pre- and postmenopausal Bantu, Caucasian, or Japanese women. Elevated plasma prolactin levels were found in women castrated prior to 35 years of age and in women whose first pregnancy occured after 35 years. Evidence indicates that the prolactin/estrogen relationship may not be similar in women 35 to 45 years of age as compared to young women and suggests that elevation of serum prolactin per se does not appear to be related to an increased risk of breast cancer. The prolactin level was increased only in Caucasian women with breast cancer. If a high prolactin/estradiol ratio increases the susceptibility of the mammary epithelium to neoplastic growth, the lack of changes in prolactin levels in premenopausal Japanese patients and in postmenopausal patients of the three ethnic groups indicates that other factors are involved. Further study of the effects of life-style and diet on the basal level and stimulated release of prolactin is required to resolve the relationship of prolactin to breast cancer.", "PMID": 975051}
{"id": "pubmed23n0032_10126", "title": "Effect of N-methyl-N-nitrosourea on the DNA of rat bladder epithelium.", "content": "Bladder cancer can be induced in the rat by the intravesicular administration of N-methyl-N-nitrosourea. DNA damage in rat bladder epithelial cells after administration of methylnitrosourea has been examined by measuring the change in sedimentation of the DNA in alkaline sucrose gradients. A dose response of DNA damage in the urothelium was observed with single intravesicular doses of 0.1, 0.3, and 0.5 mg of methylnitrosourea. Larger doses of methylnitrosourea damaged the epithelium so extensively, that biochemical studies were not feasible. DNA repair, measured by the return to a normal sedimentation pattern of DNA on alkaline sucrose gradients, was followed over a period of 9 days with the use of 0.5 mg of methylnitrosourea to initiate the damage. Bladder epithelial cells were able to repair the DNA damage induced by methylnitrosourea. However, the possibility of persistent damage not detectable by sedimentation of DNA on alkaline sucrose gradients cannot be ruled out.", "contents": "Effect of N-methyl-N-nitrosourea on the DNA of rat bladder epithelium. Bladder cancer can be induced in the rat by the intravesicular administration of N-methyl-N-nitrosourea. DNA damage in rat bladder epithelial cells after administration of methylnitrosourea has been examined by measuring the change in sedimentation of the DNA in alkaline sucrose gradients. A dose response of DNA damage in the urothelium was observed with single intravesicular doses of 0.1, 0.3, and 0.5 mg of methylnitrosourea. Larger doses of methylnitrosourea damaged the epithelium so extensively, that biochemical studies were not feasible. DNA repair, measured by the return to a normal sedimentation pattern of DNA on alkaline sucrose gradients, was followed over a period of 9 days with the use of 0.5 mg of methylnitrosourea to initiate the damage. Bladder epithelial cells were able to repair the DNA damage induced by methylnitrosourea. However, the possibility of persistent damage not detectable by sedimentation of DNA on alkaline sucrose gradients cannot be ruled out.", "PMID": 975052}
{"id": "pubmed23n0032_10127", "title": "Immunological enhancement of leukemia L1210 by Corynebacterium parvum in allogeneic mice.", "content": "The effect of Corynebacterium parvum on the immune response of C57BL/6 mice (H-2b) to the allogeneic leukemia L1210 (H-2d) was investigated. Mice were either left untreated or given C. parvum i.v. or i.p. in various dosages. Seven days later they were challenged with 2.5 to 10 X 10(6) live L1210 cells i.p. Control animals almost always rejected the challenge. In contrast, most mice pretreated with either 1.0, 0.5, or 0.25 mg of C. parvum i.v. and 1.0 or 0.5 mg i.p. exhibited enhanced growth of leukemia L1210 as indicated by gross ascites and significantly greater weight gain. This sometimes progressed to the death of the animal, but more often regressed after several days. Spleen cell-mediated cytotoxicity to alloantigens, evaluated in vitro by release of 51Cr from P815Y (H-2d) target cells, was significantly decreased in the mice pretreated with either 1.0 or 0.5 mg of C. parvum i.v. or 0.5 mg of C. parvum i.p. This suppression could not be reversed by reduction of the concentration of macrophages in the spleen cell suspensions. Complement-dependent cytotoxic antibody, measured by release of 51Cr from L1210 cells, was profoundly suppressed in mice pretreated with C. parvum i.v. in dosages ranging from 1.0 to 0.1 mg. These data suggest an immunological basis for the enhanced growth of leukemia L1210 caused by C. parvum at these schedules.", "contents": "Immunological enhancement of leukemia L1210 by Corynebacterium parvum in allogeneic mice. The effect of Corynebacterium parvum on the immune response of C57BL/6 mice (H-2b) to the allogeneic leukemia L1210 (H-2d) was investigated. Mice were either left untreated or given C. parvum i.v. or i.p. in various dosages. Seven days later they were challenged with 2.5 to 10 X 10(6) live L1210 cells i.p. Control animals almost always rejected the challenge. In contrast, most mice pretreated with either 1.0, 0.5, or 0.25 mg of C. parvum i.v. and 1.0 or 0.5 mg i.p. exhibited enhanced growth of leukemia L1210 as indicated by gross ascites and significantly greater weight gain. This sometimes progressed to the death of the animal, but more often regressed after several days. Spleen cell-mediated cytotoxicity to alloantigens, evaluated in vitro by release of 51Cr from P815Y (H-2d) target cells, was significantly decreased in the mice pretreated with either 1.0 or 0.5 mg of C. parvum i.v. or 0.5 mg of C. parvum i.p. This suppression could not be reversed by reduction of the concentration of macrophages in the spleen cell suspensions. Complement-dependent cytotoxic antibody, measured by release of 51Cr from L1210 cells, was profoundly suppressed in mice pretreated with C. parvum i.v. in dosages ranging from 1.0 to 0.1 mg. These data suggest an immunological basis for the enhanced growth of leukemia L1210 caused by C. parvum at these schedules.", "PMID": 975053}
{"id": "pubmed23n0032_10128", "title": "Aryl hydrocarbon hydroxylase in mouse mammary gland.", "content": "The specific activity of aryl hydrocarbon hydroxylase in the mammary gland has been measured in six different mouse strains comprising both mammary cancer susceptibility and resistance, under basal conditions, and after pretreatment of the mice with 3-methylcholanthrene (3MC). No correlation was observed between the ease of 3MC-enhanced mammary tumorigenesis and the basal or induced specific activity of aryl hydrocarbon hydroxylase. Furthermore, it was shown that in the AKR/J and A+/Ki mice, only minor enhancement in aryl hydrocarbon hydroxylase was seen upon injection of 3MC. The C57BL/6J and C3Hf/He Tex mammary glands responded the most in this regard. It was also shown that the inability of the AKR/J or A+/Ki mice to respond was not due to their inability to absorb 3MC from the peritoneal cavity and transmit the polycyclic hydrocarbon to the mammary gland.", "contents": "Aryl hydrocarbon hydroxylase in mouse mammary gland. The specific activity of aryl hydrocarbon hydroxylase in the mammary gland has been measured in six different mouse strains comprising both mammary cancer susceptibility and resistance, under basal conditions, and after pretreatment of the mice with 3-methylcholanthrene (3MC). No correlation was observed between the ease of 3MC-enhanced mammary tumorigenesis and the basal or induced specific activity of aryl hydrocarbon hydroxylase. Furthermore, it was shown that in the AKR/J and A+/Ki mice, only minor enhancement in aryl hydrocarbon hydroxylase was seen upon injection of 3MC. The C57BL/6J and C3Hf/He Tex mammary glands responded the most in this regard. It was also shown that the inability of the AKR/J or A+/Ki mice to respond was not due to their inability to absorb 3MC from the peritoneal cavity and transmit the polycyclic hydrocarbon to the mammary gland.", "PMID": 975054}
{"id": "pubmed23n0032_10129", "title": "Quantitative studies of the toxicity of benzo(a)pyrene to a mouse liver epithelial cell strain in culture.", "content": "The toxic effects of the carcinogen benzo(a)pyrene (BaP) were studied in a well-characterized epithelial cell strain NMuLi, derived from the livers of weanling Namru mice. These cells were extremely susceptible to the toxicity, 99% dying after a 6-day exposure to BaP, 5mug/ml. The toxic effects began between 11 and 24 hr postapplication of BaP to the cells and increased exponentially with the time of treatment. The toxicity was concentration dependent in cells treated for a specific time period. The survival curves were exponential and extrapolated to a survival fraction of 1.0. The toxic effects of BaP to logarithmically growing NMuLi were inhibited 40% by 7,8 benzoflavone, and the inhibition was concentration dependent. The 7,8-benzoflavone also inhibited aryl hydrocarbon hydroxylase (AHH) from NMuLi cell homogenates and microsomes by 99%. The concentration dependence for AHH inhibition by 7,8-benzoflavone paralleled its inhibition of cellular toxicity. The toxicity of BaP to these cells increased exponentially with the number of population doublings. Hence, the toxicity was 130 times greater in exponentially growing cells than in confluent cells. Levels of AHH, the enzyme that metabolizes BaP to its cytotoxic derivatives, were only 2.4 times higher in exponentially growing than in confluent cells, suggesting that cell division was responsible for the large differential toxicity. In addition, a toxic BaP metabolite was preferentially toxic to log-phase cells. The results indicate that the metabolism of BaP by AHH to produce cytotoxic metabolites, which may cause lesions that are expressed upon cell division, is responsible for the cytotoxicity of BaP to NMuLi.", "contents": "Quantitative studies of the toxicity of benzo(a)pyrene to a mouse liver epithelial cell strain in culture. The toxic effects of the carcinogen benzo(a)pyrene (BaP) were studied in a well-characterized epithelial cell strain NMuLi, derived from the livers of weanling Namru mice. These cells were extremely susceptible to the toxicity, 99% dying after a 6-day exposure to BaP, 5mug/ml. The toxic effects began between 11 and 24 hr postapplication of BaP to the cells and increased exponentially with the time of treatment. The toxicity was concentration dependent in cells treated for a specific time period. The survival curves were exponential and extrapolated to a survival fraction of 1.0. The toxic effects of BaP to logarithmically growing NMuLi were inhibited 40% by 7,8 benzoflavone, and the inhibition was concentration dependent. The 7,8-benzoflavone also inhibited aryl hydrocarbon hydroxylase (AHH) from NMuLi cell homogenates and microsomes by 99%. The concentration dependence for AHH inhibition by 7,8-benzoflavone paralleled its inhibition of cellular toxicity. The toxicity of BaP to these cells increased exponentially with the number of population doublings. Hence, the toxicity was 130 times greater in exponentially growing cells than in confluent cells. Levels of AHH, the enzyme that metabolizes BaP to its cytotoxic derivatives, were only 2.4 times higher in exponentially growing than in confluent cells, suggesting that cell division was responsible for the large differential toxicity. In addition, a toxic BaP metabolite was preferentially toxic to log-phase cells. The results indicate that the metabolism of BaP by AHH to produce cytotoxic metabolites, which may cause lesions that are expressed upon cell division, is responsible for the cytotoxicity of BaP to NMuLi.", "PMID": 975055}
{"id": "pubmed23n0032_10130", "title": "Induction of type B virions to bud into cytoplasmic vacuoles in a mammary tumor of an X-ray- and urethan-treated X/Gf mouse.", "content": "Electron microscopic studies of thin sections of a mammary carcinoma that developed in a female mouse of the syngeneic X/Gf low-cancer strain after treatments with X-rays (3 X 100 rads weekly) followed by 8 weekly i.p. injections of urethan (1 mg/g body weight) revealed extensive budding of B particles into cytoplasmic vacuoles and numerous characteristic B virions in the vacuolar lumens. Simultaneous budding of B particles into cytoplasmic vacuoles and budding of B particles at plasma membranes of the same cancer cell were also noted (figs. 2 to 6).", "contents": "Induction of type B virions to bud into cytoplasmic vacuoles in a mammary tumor of an X-ray- and urethan-treated X/Gf mouse. Electron microscopic studies of thin sections of a mammary carcinoma that developed in a female mouse of the syngeneic X/Gf low-cancer strain after treatments with X-rays (3 X 100 rads weekly) followed by 8 weekly i.p. injections of urethan (1 mg/g body weight) revealed extensive budding of B particles into cytoplasmic vacuoles and numerous characteristic B virions in the vacuolar lumens. Simultaneous budding of B particles into cytoplasmic vacuoles and budding of B particles at plasma membranes of the same cancer cell were also noted (figs. 2 to 6).", "PMID": 975056}
{"id": "pubmed23n0032_10131", "title": "Differentiation of early mouse embryonic and teratocarcinoma cells in vitro: plasminogen activator production.", "content": "Cultured mouse blastocysts produce plasminogen activator, a protease that converts the zymogen plasminogen into the trypsin-like enzyme, plasmin. We have fractionated the blastocyst and cultured the constituent cell types. Trophoblast outgrowths free of inner cell mass derivatives secrete plasminogen activator during a time period that closely parallels the invasive phase of trophoblast cells in utero. Isolated inner cell masses also produce plasminogen activator; further fractionation of the inner cell mass as well as studies with primary cultures obtained from midgestation tissues demonstrate that enzyme formation is restricted entirely to parietal endoderm cells. Secretion of the enzyme may facilitate the migration of parietal endoderm cells along the trophoblast layer as the yolk sac cavity enlarges during gestation. F9 embryonal carcinoma cells do not secrete detectable amounts of plasminogen activator. However, when these cells are induced to differentiate, the resulting parietal endoderm-like cells are capable of producing the enzyme. These results are consistent with previous findings suggesting that plasminogen activator production may be a characteristic of invasive and/or migratory cells.", "contents": "Differentiation of early mouse embryonic and teratocarcinoma cells in vitro: plasminogen activator production. Cultured mouse blastocysts produce plasminogen activator, a protease that converts the zymogen plasminogen into the trypsin-like enzyme, plasmin. We have fractionated the blastocyst and cultured the constituent cell types. Trophoblast outgrowths free of inner cell mass derivatives secrete plasminogen activator during a time period that closely parallels the invasive phase of trophoblast cells in utero. Isolated inner cell masses also produce plasminogen activator; further fractionation of the inner cell mass as well as studies with primary cultures obtained from midgestation tissues demonstrate that enzyme formation is restricted entirely to parietal endoderm cells. Secretion of the enzyme may facilitate the migration of parietal endoderm cells along the trophoblast layer as the yolk sac cavity enlarges during gestation. F9 embryonal carcinoma cells do not secrete detectable amounts of plasminogen activator. However, when these cells are induced to differentiate, the resulting parietal endoderm-like cells are capable of producing the enzyme. These results are consistent with previous findings suggesting that plasminogen activator production may be a characteristic of invasive and/or migratory cells.", "PMID": 975058}
{"id": "pubmed23n0032_10132", "title": "Evidence for altered gene regulation in HeLa cells returned to culture after growing in immunosuppressed rats.", "content": "HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme regulation after these cells are returned to culture for at least three months. These cells in culture demonstrate a density-dependent alteration in isoenzyme profiles. The Regan isoenzyme is the dominant isoenzyme form in new, sparsely populated cultures, while the oncoamnion isoenzyme predominates in the later high-density stages of growth. Prednisolone induction of enzyme activity is found to be most effective in the early stages of growth, and the previously reported specificity of hormone action on isoenzymes produced by cancer cells is maintained. Enzyme induction is restricted to the Regan isoenzyme, while hormone-mediated diminution in enzyme activity is confined to the oncoamnion (FL) isoenzyme.", "contents": "Evidence for altered gene regulation in HeLa cells returned to culture after growing in immunosuppressed rats. HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme regulation after these cells are returned to culture for at least three months. These cells in culture demonstrate a density-dependent alteration in isoenzyme profiles. The Regan isoenzyme is the dominant isoenzyme form in new, sparsely populated cultures, while the oncoamnion isoenzyme predominates in the later high-density stages of growth. Prednisolone induction of enzyme activity is found to be most effective in the early stages of growth, and the previously reported specificity of hormone action on isoenzymes produced by cancer cells is maintained. Enzyme induction is restricted to the Regan isoenzyme, while hormone-mediated diminution in enzyme activity is confined to the oncoamnion (FL) isoenzyme.", "PMID": 975060}
{"id": "pubmed23n0032_10133", "title": "Alkaline phosphatase isoenzymes in intestinal metaplasia and carcinoma of the stomach.", "content": "The enzymological and immunological properties of alkaline phosphatase (ALP) isoenzymes in intestinal metaplasia and gastric carcinoma tissues were compared with those of purified human intestinal, placental, and hepatic ALP's to speculate the gene expression of gastric cancer cells. The results were as follows: Intestinal metaplasia was found in 21 of 23 cases (91%) of gastric cancer, whereas normal gastric mucosa had no ALP activity. ALP extracted from the mucosa of intestinal metaplasia was identical with intestinal ALP as to enzymological and immunological properties. ALP obtained from gastric carcinoma tissues of 23 patients was separated into 3 bands (ALPa, ALPb, and ALPc) by polyacrylamide-gel disc electrophoresis. Ten cases showed ALPa only, 4 cases showed ALPa and ALPb, 5 cases showed ALPa and ALPc, and the other 4 cases showed ALPa, ALPb, ALPc, respectively. It was concluded that ALPa was similar in its enzymological and immunological properties to hepatic-type ALP, ALPb was similar to placental-type ALP, and ALPc was similar to intestinal-type ALP, ALPb probably originates from the cancer cell itself. As for the other enzymes, ALPa and ALPc, further investigation should be made to determine whether they come from the cancer cell itself or not.", "contents": "Alkaline phosphatase isoenzymes in intestinal metaplasia and carcinoma of the stomach. The enzymological and immunological properties of alkaline phosphatase (ALP) isoenzymes in intestinal metaplasia and gastric carcinoma tissues were compared with those of purified human intestinal, placental, and hepatic ALP's to speculate the gene expression of gastric cancer cells. The results were as follows: Intestinal metaplasia was found in 21 of 23 cases (91%) of gastric cancer, whereas normal gastric mucosa had no ALP activity. ALP extracted from the mucosa of intestinal metaplasia was identical with intestinal ALP as to enzymological and immunological properties. ALP obtained from gastric carcinoma tissues of 23 patients was separated into 3 bands (ALPa, ALPb, and ALPc) by polyacrylamide-gel disc electrophoresis. Ten cases showed ALPa only, 4 cases showed ALPa and ALPb, 5 cases showed ALPa and ALPc, and the other 4 cases showed ALPa, ALPb, ALPc, respectively. It was concluded that ALPa was similar in its enzymological and immunological properties to hepatic-type ALP, ALPb was similar to placental-type ALP, and ALPc was similar to intestinal-type ALP, ALPb probably originates from the cancer cell itself. As for the other enzymes, ALPa and ALPc, further investigation should be made to determine whether they come from the cancer cell itself or not.", "PMID": 975061}
{"id": "pubmed23n0032_10134", "title": "A model for the control of transcription during development.", "content": "In order to account for the coordinate expression of large numbers of genes that must occur during development and differentiation, a mechanism of transcription control distinct from those operating in bacteria is proposed. Transcription would be initiated by small RNA chains that would function as primers for elongation. A given species of primer RNA generated by a primary induction event would bind by complementary base pairing to a variety of sites in the genome and thus trigger the transcription of adjacent structural genes. The primer RNA region would be excised from the transcription products in the nucleus, and possibly be reutilized as primers for transcription. This model can account for the occurrence of tissue-specific nuclear RNA complementary to repetitive DNA and does not require that the major RNA polymerases be capable of initiation. A minor RNA polymerase capable of initiating primer RNA chains and subject to conventional transcription controls would be required. Carcinogenesis, which is accompanied in many cases by the appearance of a variety of embryonic antigens, could involve the induction of RNA primers normally programmed to function early in development.", "contents": "A model for the control of transcription during development. In order to account for the coordinate expression of large numbers of genes that must occur during development and differentiation, a mechanism of transcription control distinct from those operating in bacteria is proposed. Transcription would be initiated by small RNA chains that would function as primers for elongation. A given species of primer RNA generated by a primary induction event would bind by complementary base pairing to a variety of sites in the genome and thus trigger the transcription of adjacent structural genes. The primer RNA region would be excised from the transcription products in the nucleus, and possibly be reutilized as primers for transcription. This model can account for the occurrence of tissue-specific nuclear RNA complementary to repetitive DNA and does not require that the major RNA polymerases be capable of initiation. A minor RNA polymerase capable of initiating primer RNA chains and subject to conventional transcription controls would be required. Carcinogenesis, which is accompanied in many cases by the appearance of a variety of embryonic antigens, could involve the induction of RNA primers normally programmed to function early in development.", "PMID": 975062}
{"id": "pubmed23n0032_10135", "title": "The role of nuclei and nucleoli in the control of cell proliferation.", "content": "A number of changes have been reported to occur in chromatin of quiescent cells stimulated to proliferate. These changes, indicative of increased transcriptional activity, occur in the early prereplicative phase, several hours before the onset of DNA replication, and are detectable not only in chromatin, but also in isolated nuclei and in whole cells. Most of the increased transcriptional activity can be attributed to an increased activity of the nucleolus; however, extranucleolar genes are also important in the regulation of the cell cycle flow, from G0 to S.", "contents": "The role of nuclei and nucleoli in the control of cell proliferation. A number of changes have been reported to occur in chromatin of quiescent cells stimulated to proliferate. These changes, indicative of increased transcriptional activity, occur in the early prereplicative phase, several hours before the onset of DNA replication, and are detectable not only in chromatin, but also in isolated nuclei and in whole cells. Most of the increased transcriptional activity can be attributed to an increased activity of the nucleolus; however, extranucleolar genes are also important in the regulation of the cell cycle flow, from G0 to S.", "PMID": 975063}
{"id": "pubmed23n0032_10136", "title": "Role of nonhistone chromosomal proteins in the regulation of histone gene expression.", "content": "Histone gene expression was studied during the cell cycle of continuously dividing HeLa S3-cells and following stimulation of confluent monolayers of WI-38 human diploid fibroblasts to proliferate. The presence of histone messenger RNA (mRNA) sequences was assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNA's. In HeLa S3-cells, histone mRNA sequences were found in the nucleus and associated with polyribosomes during S phase but not during G1. Transcripts of S-phase chromatin contained histone mRNA sequences but those of G1 chromatin did not. Similarly, in WI-38 cells association of histone mRNA sequences with polyribosomes and transcription of histone mRNA sequences from chromatin parallel DNA replication. Together these results suggest that the regulation of histone gene expression resides, at least in part, at the transcriptional level. Chromatin reconstitution studies provide evidence that nonhistone chromosomal proteins play a key role in activation of histone gene transcription during the period of the cell cycle when DNA is replicated. Phosphate groups associated with the S-phase nonhistone chromosomal proteins appear to be functionally involved in the control of histone gene readout. Although WI-38 human diploid fibroblasts transformed by SV40 exhibit morphological and biochemical modifications characteristic of neoplastic cells, transcription of histone mRNA sequences remains unaltered.", "contents": "Role of nonhistone chromosomal proteins in the regulation of histone gene expression. Histone gene expression was studied during the cell cycle of continuously dividing HeLa S3-cells and following stimulation of confluent monolayers of WI-38 human diploid fibroblasts to proliferate. The presence of histone messenger RNA (mRNA) sequences was assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNA's. In HeLa S3-cells, histone mRNA sequences were found in the nucleus and associated with polyribosomes during S phase but not during G1. Transcripts of S-phase chromatin contained histone mRNA sequences but those of G1 chromatin did not. Similarly, in WI-38 cells association of histone mRNA sequences with polyribosomes and transcription of histone mRNA sequences from chromatin parallel DNA replication. Together these results suggest that the regulation of histone gene expression resides, at least in part, at the transcriptional level. Chromatin reconstitution studies provide evidence that nonhistone chromosomal proteins play a key role in activation of histone gene transcription during the period of the cell cycle when DNA is replicated. Phosphate groups associated with the S-phase nonhistone chromosomal proteins appear to be functionally involved in the control of histone gene readout. Although WI-38 human diploid fibroblasts transformed by SV40 exhibit morphological and biochemical modifications characteristic of neoplastic cells, transcription of histone mRNA sequences remains unaltered.", "PMID": 975064}
{"id": "pubmed23n0032_10137", "title": "Fundamental carcinogenic processes and their implications for low dose risk assessment.", "content": "Various possible models of carcinogenesis are analyzed with respect to low dose kinetics. The importance of background carcinogenesis upon the shape of the dose-response curve at low dose is emphasized. It is shown that, if carcinogenesis by an external agent acts additively with any already ongoing process, then under almost any model the response will be linear at low dose. Measures of the degree of linearity are obtained for multistage models of carcinogenesis, where it is shown that throughout the dose range where the extra risk is less than the spontaneous risk linear extrapolation must be quite accurate.", "contents": "Fundamental carcinogenic processes and their implications for low dose risk assessment. Various possible models of carcinogenesis are analyzed with respect to low dose kinetics. The importance of background carcinogenesis upon the shape of the dose-response curve at low dose is emphasized. It is shown that, if carcinogenesis by an external agent acts additively with any already ongoing process, then under almost any model the response will be linear at low dose. Measures of the degree of linearity are obtained for multistage models of carcinogenesis, where it is shown that throughout the dose range where the extra risk is less than the spontaneous risk linear extrapolation must be quite accurate.", "PMID": 975067}
{"id": "pubmed23n0032_10138", "title": "Use of lipid vesicles as carriers to introduce actinomycin D into resistant tumor cells.", "content": "Unilamellar lipid vesicles have been used as a carrier vehicle to enhance the uptake of actinomycin D into an actinomycin D-resistant Chinese hamster tumor cell line (DC-3F/ADX). The DC-3F/ADX cell line is resistant to actinomycin D as a result of its decreased capacity to transport actinomycin D across the plasma membrane and is able to grow in the presence of concentrations of actinomycin D that are cytotoxic for the sensitive parent cell line (DC-3F). Incubation of resistant DC-3F/ADX cells with actinomycin D-containing vesicles produced a 5-fold increase in intracellular drug concentration over that achieved by exposure to identical concentrations of the drug added to the culture medium. Vesicle-mediated uptake of actinomycin D into resistant cells produced inhibition of cellular RNA synthesis and cell growth at actinomycin D concentrations that had no inhibitory effect when added as free drug in the culture medium. Dose-response measurements established that direct introduction of actinomycin D into resistant cells by means of lipid vesicles resulted in a 200-fold reduction in the concentration of actinomycin D required to inhibit cellular RNA synthesis and a 120-fold reduction in the dose of actinomycin D required to produce a 50% inhibition of cell growth. These results lend strong support to the hypothesis that cellular resistant to actinomycin D is due to a lower capacity to take up actinomycin D as a result of a reduction in the permeability of the cellular plasma membrane to this drug. The potential value of lipid vesicles for introducing other classes of drugs into cultured cells and their possible use in chemotherapy are also discussed.", "contents": "Use of lipid vesicles as carriers to introduce actinomycin D into resistant tumor cells. Unilamellar lipid vesicles have been used as a carrier vehicle to enhance the uptake of actinomycin D into an actinomycin D-resistant Chinese hamster tumor cell line (DC-3F/ADX). The DC-3F/ADX cell line is resistant to actinomycin D as a result of its decreased capacity to transport actinomycin D across the plasma membrane and is able to grow in the presence of concentrations of actinomycin D that are cytotoxic for the sensitive parent cell line (DC-3F). Incubation of resistant DC-3F/ADX cells with actinomycin D-containing vesicles produced a 5-fold increase in intracellular drug concentration over that achieved by exposure to identical concentrations of the drug added to the culture medium. Vesicle-mediated uptake of actinomycin D into resistant cells produced inhibition of cellular RNA synthesis and cell growth at actinomycin D concentrations that had no inhibitory effect when added as free drug in the culture medium. Dose-response measurements established that direct introduction of actinomycin D into resistant cells by means of lipid vesicles resulted in a 200-fold reduction in the concentration of actinomycin D required to inhibit cellular RNA synthesis and a 120-fold reduction in the dose of actinomycin D required to produce a 50% inhibition of cell growth. These results lend strong support to the hypothesis that cellular resistant to actinomycin D is due to a lower capacity to take up actinomycin D as a result of a reduction in the permeability of the cellular plasma membrane to this drug. The potential value of lipid vesicles for introducing other classes of drugs into cultured cells and their possible use in chemotherapy are also discussed.", "PMID": 975068}
{"id": "pubmed23n0032_10139", "title": "A rapid assay for stimulation of human lymphocytes by tumor-associated antigens.", "content": "Lymphocyte-stimulated protein synthesis (SPS) in response to human tumor-associated antigens was assessed by measuring [3H]leucine incorporation. Correlation of SPS with other in vivo and in vitro response was demonstrated by immunizing normal subjects with keyhole limpet hemocyanin and testing sequentially frozen lymphocytes and serum samples. One week after immunization, lymphocytes from normal subjects demonstrated increased SPS to keyhole limpet hemocyanin. This correlated with the appearance of delayed cutaneous hypersensitivity responses and preceded detection of hemagglutinating antibodies and increases in lymphocyte [3H]thymidine incorporation. There was no difference in the reactivity of fresh and viable frozen lymphocytes, and as few as 5X 10(5) lymphocytes/microtiter plate well could be used. Tumor-associated antigens were prepared from four lung carcinomas, six sarcomas, and six melanomas, using 3 M KCI extraction. Lymphocyte responses to both autologous and allogeneic tumor extracts were observed. Five of 15 patients demonstrated significant SPS to autologous tumor antigens. Fourteen of 20 lung cancer patients responded to lung cancer antigen, whereas only 11 of 41 patients with other tumors and 3 of 19 normal subjects reacted. Significantly, more lung cancer patients reacted to the tumor extract than to an extract of uninvolved lung from the same patient. Twenty-one of 42 melanoma patients responded to melanoma antigen. Ten of 33 patients with other tumors and 3 of 24 normal subjects reacted to the melanoma extract. Eight of 30 melanoma patients reacted to an extract of muscle from the same donor as was the melanoma antigen. Tumor-associated antigenic activity of 3 M KCI extracts can therefore be detected by measuring lymphocyte [3h]leucine incorporation.", "contents": "A rapid assay for stimulation of human lymphocytes by tumor-associated antigens. Lymphocyte-stimulated protein synthesis (SPS) in response to human tumor-associated antigens was assessed by measuring [3H]leucine incorporation. Correlation of SPS with other in vivo and in vitro response was demonstrated by immunizing normal subjects with keyhole limpet hemocyanin and testing sequentially frozen lymphocytes and serum samples. One week after immunization, lymphocytes from normal subjects demonstrated increased SPS to keyhole limpet hemocyanin. This correlated with the appearance of delayed cutaneous hypersensitivity responses and preceded detection of hemagglutinating antibodies and increases in lymphocyte [3H]thymidine incorporation. There was no difference in the reactivity of fresh and viable frozen lymphocytes, and as few as 5X 10(5) lymphocytes/microtiter plate well could be used. Tumor-associated antigens were prepared from four lung carcinomas, six sarcomas, and six melanomas, using 3 M KCI extraction. Lymphocyte responses to both autologous and allogeneic tumor extracts were observed. Five of 15 patients demonstrated significant SPS to autologous tumor antigens. Fourteen of 20 lung cancer patients responded to lung cancer antigen, whereas only 11 of 41 patients with other tumors and 3 of 19 normal subjects reacted. Significantly, more lung cancer patients reacted to the tumor extract than to an extract of uninvolved lung from the same patient. Twenty-one of 42 melanoma patients responded to melanoma antigen. Ten of 33 patients with other tumors and 3 of 24 normal subjects reacted to the melanoma extract. Eight of 30 melanoma patients reacted to an extract of muscle from the same donor as was the melanoma antigen. Tumor-associated antigenic activity of 3 M KCI extracts can therefore be detected by measuring lymphocyte [3h]leucine incorporation.", "PMID": 975069}
{"id": "pubmed23n0032_10140", "title": "Effects of single cyclophosphamide doses on the kinetics of thoracic duct lymph and blood leukocytes in calves.", "content": "After 5 or 20 mg cyclophosphamide per kg body weight, given once i.v., the output in thoracic duct lymph of small and large lymphoid cells, cells incorporating [3H]lymidine in vitro, mitotic cells, pyknotic cells, and/or the number of lymphocytes and neutrophils in peripheral blood were measured in six calves. The median grain count of labeled cells and the DNA content of pyknotic nucleic were determined. After both doses there was an exponential decrease and subsequent recovery of the median grain count. The larger dose caused a temporary cessation of lymphoid cell division, reduced the output of nondividing small lymphoid cells, and probably imparied proliferation of neutrophil precursors. The results suggest that increased cell production during recovery was due to changes in the growth fraction and that feedback mechanisms acting on G0-G1 cells controled the proliferation of lymphoid cells.", "contents": "Effects of single cyclophosphamide doses on the kinetics of thoracic duct lymph and blood leukocytes in calves. After 5 or 20 mg cyclophosphamide per kg body weight, given once i.v., the output in thoracic duct lymph of small and large lymphoid cells, cells incorporating [3H]lymidine in vitro, mitotic cells, pyknotic cells, and/or the number of lymphocytes and neutrophils in peripheral blood were measured in six calves. The median grain count of labeled cells and the DNA content of pyknotic nucleic were determined. After both doses there was an exponential decrease and subsequent recovery of the median grain count. The larger dose caused a temporary cessation of lymphoid cell division, reduced the output of nondividing small lymphoid cells, and probably imparied proliferation of neutrophil precursors. The results suggest that increased cell production during recovery was due to changes in the growth fraction and that feedback mechanisms acting on G0-G1 cells controled the proliferation of lymphoid cells.", "PMID": 975070}
{"id": "pubmed23n0032_10141", "title": "Kinetics of the plasmacellular reaction in the draining lymph node of mice bearing the BP-8 fibrosarcoma.", "content": "The kinetics of the plasmacellular response in the draining lymph node was quantitatively assessed in specific-pathogen-free C3H/He mice grafted S.C. with the syngeneic BP-8 fibrosarcoma. The percentage of plasma cells was estimated on dissociated, cytocentrifuged, and fixed cell preparations by immunoenzymatic staining with either of two B-cell-specific antibody markers. Mice either grafted with liver tumor or given a single implant of tumor homogenate gave a maximal plasma cell response of about 4%. However, this peak was reached at 14 days in the live grafts and at 7 days in the homogenate situations. Moreover, in animals grafted with live tumor an elevated 3% plateau persisted until death, wheras in homogenate-implanted animals a sharp drop to a 0.5% plateau was observed at about Day 20. Homogenate-treated animals displayed a significant enhancement of tumor growth when challenged with a live graft 7 days after homogenate implantation, i.e., during the peak 4% plasmacellular response. Mice given a single implant of X-irradiated (3500 R) tumor responded with a peak of 1.7% plasma cells at 7 days. Despite subsequent boosting with the same material (Days 14 and 21), this value declined and remained at a plateau of about 0.2% until death. Contrary to homogenate-treated mice, these animals were immune to subsequent challenge with a live tumor graft. In the present host-tumor system, there appears to be an inverse relationship between elevated numbers of plasma cells and antitumor immunity.", "contents": "Kinetics of the plasmacellular reaction in the draining lymph node of mice bearing the BP-8 fibrosarcoma. The kinetics of the plasmacellular response in the draining lymph node was quantitatively assessed in specific-pathogen-free C3H/He mice grafted S.C. with the syngeneic BP-8 fibrosarcoma. The percentage of plasma cells was estimated on dissociated, cytocentrifuged, and fixed cell preparations by immunoenzymatic staining with either of two B-cell-specific antibody markers. Mice either grafted with liver tumor or given a single implant of tumor homogenate gave a maximal plasma cell response of about 4%. However, this peak was reached at 14 days in the live grafts and at 7 days in the homogenate situations. Moreover, in animals grafted with live tumor an elevated 3% plateau persisted until death, wheras in homogenate-implanted animals a sharp drop to a 0.5% plateau was observed at about Day 20. Homogenate-treated animals displayed a significant enhancement of tumor growth when challenged with a live graft 7 days after homogenate implantation, i.e., during the peak 4% plasmacellular response. Mice given a single implant of X-irradiated (3500 R) tumor responded with a peak of 1.7% plasma cells at 7 days. Despite subsequent boosting with the same material (Days 14 and 21), this value declined and remained at a plateau of about 0.2% until death. Contrary to homogenate-treated mice, these animals were immune to subsequent challenge with a live tumor graft. In the present host-tumor system, there appears to be an inverse relationship between elevated numbers of plasma cells and antitumor immunity.", "PMID": 975071}
{"id": "pubmed23n0032_10142", "title": "Increased attachment of nucleic acids to eukaryotic and prokaryotic cells induced by chemical and physical carcinogens and mutagens.", "content": "Significantly enhanced attachment to Ehrlich ascites and Escherichia coli cells was observed for radioactive DNA and RNA in the presence of chemical mutagens and ultimate carcinogens. In some instances, formation of nucleic acid-protein adducts by these compounds further (or similarly) enhanced the binding. DNA irradiated with ultraviolet light in the presence of a protein bound more efficiently than either an unirradiated mixture of these two macromolecules or DNA irradiated alone. The spectrum of compounds tested and found active in this system includes alkylating agents, aromatic amines, and carcinogenic metals. Precarcinogens and nonultimate carcinogenic chemicals, as well as tumor-promoting agents, did not increase the binding. However, addition of extracts from mouse or rat livers activated precarcinogenic and proximate carcinogenic chemicals  and resulted in enhanced cellular attachment of indicator nucleic acids in their presence. Possible usefulness of this test system for fast and efficient screening for environmental carcinogens and mutagens, as well as possible relevance of the observed phenomena to in vivo effects of chemical and physical carcinogens, is considered.", "contents": "Increased attachment of nucleic acids to eukaryotic and prokaryotic cells induced by chemical and physical carcinogens and mutagens. Significantly enhanced attachment to Ehrlich ascites and Escherichia coli cells was observed for radioactive DNA and RNA in the presence of chemical mutagens and ultimate carcinogens. In some instances, formation of nucleic acid-protein adducts by these compounds further (or similarly) enhanced the binding. DNA irradiated with ultraviolet light in the presence of a protein bound more efficiently than either an unirradiated mixture of these two macromolecules or DNA irradiated alone. The spectrum of compounds tested and found active in this system includes alkylating agents, aromatic amines, and carcinogenic metals. Precarcinogens and nonultimate carcinogenic chemicals, as well as tumor-promoting agents, did not increase the binding. However, addition of extracts from mouse or rat livers activated precarcinogenic and proximate carcinogenic chemicals  and resulted in enhanced cellular attachment of indicator nucleic acids in their presence. Possible usefulness of this test system for fast and efficient screening for environmental carcinogens and mutagens, as well as possible relevance of the observed phenomena to in vivo effects of chemical and physical carcinogens, is considered.", "PMID": 975072}
{"id": "pubmed23n0032_10143", "title": "Retention of tritiated methotrexate in a transplantable mouse glioma.", "content": "The uptake and distribution of tritiated methotrexate ([H]MTX) were studied by autoradiography and liquid scintillation countinf for up to 7 days after i.v. injection in mice bearing s.c. or intracerebral (i.c.) implants of a transplantable mouse ependymoblastoma. Autoradiography showed that s.c. tumors took up more [3H]MTX than i.c. tumors. In both s.c. and i.c. tumors, [3H]MTX was mainly intracellular, with very little in intersitial fluid sites. Retention of [3H]MTX gradually diminished with time, with some still being present 7 days after injection. The distribution of [3H]MTX in the i.c. tumors was not uniform, and portions of these tumors were relatively inaccessible to the drug. The uniformity of distribution did not improve with time. Scintillation counting showed that s.c. tumors accumulated much less [3H-A1MTX and retained a lower proportion of [3H]MTX than many normal tissues. These studies indicate that [3H]MTX has 3 major shortcomings as a chemotherapeutic agent for brain tumors. First, the amount of drug taken up by the transplantable mouse ependymoblastoma was small in comparison with normal tissues and in comparison with other agents taken up by this tumor. Second, the distribution of the drug in the i.c. tumors was nonuniform, with tumor cells in certain areas remaining relatively inaccessible to the drug. Last, the retention of the drug in the tumor was far less than in normal tissues.", "contents": "Retention of tritiated methotrexate in a transplantable mouse glioma. The uptake and distribution of tritiated methotrexate ([H]MTX) were studied by autoradiography and liquid scintillation countinf for up to 7 days after i.v. injection in mice bearing s.c. or intracerebral (i.c.) implants of a transplantable mouse ependymoblastoma. Autoradiography showed that s.c. tumors took up more [3H]MTX than i.c. tumors. In both s.c. and i.c. tumors, [3H]MTX was mainly intracellular, with very little in intersitial fluid sites. Retention of [3H]MTX gradually diminished with time, with some still being present 7 days after injection. The distribution of [3H]MTX in the i.c. tumors was not uniform, and portions of these tumors were relatively inaccessible to the drug. The uniformity of distribution did not improve with time. Scintillation counting showed that s.c. tumors accumulated much less [3H-A1MTX and retained a lower proportion of [3H]MTX than many normal tissues. These studies indicate that [3H]MTX has 3 major shortcomings as a chemotherapeutic agent for brain tumors. First, the amount of drug taken up by the transplantable mouse ependymoblastoma was small in comparison with normal tissues and in comparison with other agents taken up by this tumor. Second, the distribution of the drug in the i.c. tumors was nonuniform, with tumor cells in certain areas remaining relatively inaccessible to the drug. Last, the retention of the drug in the tumor was far less than in normal tissues.", "PMID": 975073}
{"id": "pubmed23n0032_10144", "title": "Antitumor activity of 9-hydroxyellipticine (NSC 210717) ON L1210 mouse leukemia and the effect of route of injection.", "content": "The antitumor activity of a new derivative of ellipticine, 9-hydroxyellipticine (NSC 210717), was studied using L1210 mouse leukemia. Low doses of this drug have a high antileukemic activity, whereas high doses have less activity than expected because of a leveling off in the antitumor activity-dose relationship, as if a few cells were resistant to the treatment. The possible causes of this apparent resistance were investigated. It is suggested that this apparent resistance is related to the sequestration of a small number of cells in compartments inaccessible to the drug. A model was developed which takes into account the distribution of cells in various compartments and their drug sensitivity therein. It was predicted and observed that the activity of drugs acting on cells in the small compartments can be observed only in conjunction with the presence of drugs acting on the cells in the major compartment. The importance of this observation in the screening procedures of new drugs, the clinical trial of new chemotherapeutic agents, and the association of anticancer drugs are discussed within this context. 9-Hydroxyellipticine is of interest because it acts on leukemic cells in the brain.", "contents": "Antitumor activity of 9-hydroxyellipticine (NSC 210717) ON L1210 mouse leukemia and the effect of route of injection. The antitumor activity of a new derivative of ellipticine, 9-hydroxyellipticine (NSC 210717), was studied using L1210 mouse leukemia. Low doses of this drug have a high antileukemic activity, whereas high doses have less activity than expected because of a leveling off in the antitumor activity-dose relationship, as if a few cells were resistant to the treatment. The possible causes of this apparent resistance were investigated. It is suggested that this apparent resistance is related to the sequestration of a small number of cells in compartments inaccessible to the drug. A model was developed which takes into account the distribution of cells in various compartments and their drug sensitivity therein. It was predicted and observed that the activity of drugs acting on cells in the small compartments can be observed only in conjunction with the presence of drugs acting on the cells in the major compartment. The importance of this observation in the screening procedures of new drugs, the clinical trial of new chemotherapeutic agents, and the association of anticancer drugs are discussed within this context. 9-Hydroxyellipticine is of interest because it acts on leukemic cells in the brain.", "PMID": 975074}
{"id": "pubmed23n0032_10145", "title": "Combined adriamycin and hyperthermia treatment of a murine mammary carcinoma in vivo.", "content": "A study was made of the effect of combined adriamycin and hyperthermic treatment in a solid mouse mammary carcinoma in vivo. This study demonstrated: (a) that, when given separately, adriamycin and hyperthermia enhance the destruction of a solid mouse mammary carcinoma in vivo; hyperthermia (40.5-42.5 degreesy greatly increases tumor destruction and, in a number of cases, caused initial and long-time regression; (c) that whole-body hyperthermia in combination with adriamycin gives a significant delay in tumor growth as compared with the controls, but not to the same degree as the local combined therapy; and (d) that treatment with local hyperthermia and adriamycin gives a pronounced decrease in the lethal toxicitity of adriamycin. The effect of adriamycin and heat treatment may be due to hyperthermic cell destruction in the central area of the solid tumor, together with a synergistic effect of heat and adriamycin on the proliferating peripheral tumor cells. Furthermore, local heat application may increase the adriamycin concentration in the heated tumor area, which causes a high destructive effect and a less toxic influence on the nonheated normal tissue.", "contents": "Combined adriamycin and hyperthermia treatment of a murine mammary carcinoma in vivo. A study was made of the effect of combined adriamycin and hyperthermic treatment in a solid mouse mammary carcinoma in vivo. This study demonstrated: (a) that, when given separately, adriamycin and hyperthermia enhance the destruction of a solid mouse mammary carcinoma in vivo; hyperthermia (40.5-42.5 degreesy greatly increases tumor destruction and, in a number of cases, caused initial and long-time regression; (c) that whole-body hyperthermia in combination with adriamycin gives a significant delay in tumor growth as compared with the controls, but not to the same degree as the local combined therapy; and (d) that treatment with local hyperthermia and adriamycin gives a pronounced decrease in the lethal toxicitity of adriamycin. The effect of adriamycin and heat treatment may be due to hyperthermic cell destruction in the central area of the solid tumor, together with a synergistic effect of heat and adriamycin on the proliferating peripheral tumor cells. Furthermore, local heat application may increase the adriamycin concentration in the heated tumor area, which causes a high destructive effect and a less toxic influence on the nonheated normal tissue.", "PMID": 975075}
{"id": "pubmed23n0032_10146", "title": "Ultrastruct and biochemical chantes in cultured human malignant melanoma cells after heterotransplantation into nude mice.", "content": "Cells from three lines of cultured human malignant melanomas were heterotransplanted into nude mice and then recultered. The shape of the cells, the aspect of the melanosomes, and the content of 5-S-cyteinyldopa showed pronounced changes induced by the transplantation. Such results indicate that this experimental model should be used with great caution. A relationship was found between the shape of the melanosomes and the content of 5-S-cysteinyldopa in the cells.", "contents": "Ultrastruct and biochemical chantes in cultured human malignant melanoma cells after heterotransplantation into nude mice. Cells from three lines of cultured human malignant melanomas were heterotransplanted into nude mice and then recultered. The shape of the cells, the aspect of the melanosomes, and the content of 5-S-cyteinyldopa showed pronounced changes induced by the transplantation. Such results indicate that this experimental model should be used with great caution. A relationship was found between the shape of the melanosomes and the content of 5-S-cysteinyldopa in the cells.", "PMID": 975076}
{"id": "pubmed23n0032_10147", "title": "Transport of anionic substrates and glutamate metabolism in mitochondria from ascites tumor cells.", "content": "A study is presented of alpha-oxoglutarate and glutamate transport and of glutamate oxidation in ascites tumor cell mitochondria. Kinetics analysis of alpha-oxoglutarate transport in mitochondria from two strains of Ehrlich ascites tumor cells, the hyperdiploid and the hyperdiploid Lettr\u00e9 mutant, shows that the activity of the alpha-oxoglutarate carrier and its affinityfor substrates are higher in the mutant than in the wild strain. Evidence is presented showing the occurrence of carrier mediated glutamate-OH-exchange-diffusion in mitochondria from both strains. The activity of the glutamate carrier is apparently higher in the hyperdiploid Lettr\u00e9 mutant. Glutamate oxidation occurs mainly through transamination to asparatate in both tumor stains. The rate of deamination in the two strains correlates directly with the level of glutamide dehydrogenase (EC 1.4.1.3.), which is higher in the wild than in the mutant strain. Thus glutamate dehydrogenase per se, and not glutamate penetration, constitutes the control step for gluttochondria of glutamate with externally added oxaloacetate (arsenite present) that exclude an obligatory transport of oxaloacetate on the alpha-oxoglutarate carrier.", "contents": "Transport of anionic substrates and glutamate metabolism in mitochondria from ascites tumor cells. A study is presented of alpha-oxoglutarate and glutamate transport and of glutamate oxidation in ascites tumor cell mitochondria. Kinetics analysis of alpha-oxoglutarate transport in mitochondria from two strains of Ehrlich ascites tumor cells, the hyperdiploid and the hyperdiploid Lettr\u00e9 mutant, shows that the activity of the alpha-oxoglutarate carrier and its affinityfor substrates are higher in the mutant than in the wild strain. Evidence is presented showing the occurrence of carrier mediated glutamate-OH-exchange-diffusion in mitochondria from both strains. The activity of the glutamate carrier is apparently higher in the hyperdiploid Lettr\u00e9 mutant. Glutamate oxidation occurs mainly through transamination to asparatate in both tumor stains. The rate of deamination in the two strains correlates directly with the level of glutamide dehydrogenase (EC 1.4.1.3.), which is higher in the wild than in the mutant strain. Thus glutamate dehydrogenase per se, and not glutamate penetration, constitutes the control step for gluttochondria of glutamate with externally added oxaloacetate (arsenite present) that exclude an obligatory transport of oxaloacetate on the alpha-oxoglutarate carrier.", "PMID": 975077}
{"id": "pubmed23n0032_10148", "title": "Cell cycle and morphological changes during growth and differentiation of a rat basophilic leukemia cell line.", "content": "Cells of the rat basophilic leukemia cell line RBL-1 differentiated maximally when permitted to achieve growth arrest in a high-density stationary phase, in which the cell number is constant, and the cells are arrested in a G phase of the cycle. Features of differentiation are the accumulation of large basophilic granules and increases in membrane receptors for immunoglobulin E. However, changes in histamine content did not parallel granule development or changes in immunoglobulin receptor concentration. During rapid \"forced exponential\" growth, the cell number doubles every 8 hr, 50% of the cells are in S phase, and differentiation is minimal.", "contents": "Cell cycle and morphological changes during growth and differentiation of a rat basophilic leukemia cell line. Cells of the rat basophilic leukemia cell line RBL-1 differentiated maximally when permitted to achieve growth arrest in a high-density stationary phase, in which the cell number is constant, and the cells are arrested in a G phase of the cycle. Features of differentiation are the accumulation of large basophilic granules and increases in membrane receptors for immunoglobulin E. However, changes in histamine content did not parallel granule development or changes in immunoglobulin receptor concentration. During rapid \"forced exponential\" growth, the cell number doubles every 8 hr, 50% of the cells are in S phase, and differentiation is minimal.", "PMID": 975078}
{"id": "pubmed23n0032_10149", "title": "A comparison of the loosely bound and tightly bound nonhistone proteins from Ehrlich ascites tumor chromatin.", "content": "The nonhistone proteins of Ehrlich ascites tumor chromatin have been separated into a loosely bound and two tightly bound protein fractions by sequential extraction of chromatin with 0.35 M NaCl and 2 M NaCl:5 M urea. The nonhistone proteins thus obtained were examined for their chemical composition and distribution of DNA polymerase, RNA polymerase, and protein kinase activities. In addition, the effect of these nonhistone proteins on transcription of DNA in vitro has been determined. The results indicate that these nonhistone proteins, fractionated on the basis of their extractability, exhibit varied compositional characteristics and play different functional roles in the synthesis of DNA and RNA and in the possible control of gene activity.", "contents": "A comparison of the loosely bound and tightly bound nonhistone proteins from Ehrlich ascites tumor chromatin. The nonhistone proteins of Ehrlich ascites tumor chromatin have been separated into a loosely bound and two tightly bound protein fractions by sequential extraction of chromatin with 0.35 M NaCl and 2 M NaCl:5 M urea. The nonhistone proteins thus obtained were examined for their chemical composition and distribution of DNA polymerase, RNA polymerase, and protein kinase activities. In addition, the effect of these nonhistone proteins on transcription of DNA in vitro has been determined. The results indicate that these nonhistone proteins, fractionated on the basis of their extractability, exhibit varied compositional characteristics and play different functional roles in the synthesis of DNA and RNA and in the possible control of gene activity.", "PMID": 975079}
{"id": "pubmed23n0032_10150", "title": "Effects of the immunosuppressive drug niridazole in isogeneic and allogeneic mouse tumor systems in vivo.", "content": "In both isogeneic (Sarcoma 1 in A/JAX mice) and allogeneic (Sarcoma 180 in C57BL/6 mice) mouse tumor systems, treatment of the tumor-bearing mice with niridazole, an antiprarasitic drug, known to be a potent suppressor of cell mediated but not humoral immunity caused enhancement of metastases to regional popliteal nodes. Niridazole also inhibited tumor growth in vivo, as manifested by a significant decrease in the weight of the primary tumors. The enhancement of metastases is attributed to the suppression of cell-mediated immunity by the drug, but the mechanism of tumor-growth inhibition is not yet clear.", "contents": "Effects of the immunosuppressive drug niridazole in isogeneic and allogeneic mouse tumor systems in vivo. In both isogeneic (Sarcoma 1 in A/JAX mice) and allogeneic (Sarcoma 180 in C57BL/6 mice) mouse tumor systems, treatment of the tumor-bearing mice with niridazole, an antiprarasitic drug, known to be a potent suppressor of cell mediated but not humoral immunity caused enhancement of metastases to regional popliteal nodes. Niridazole also inhibited tumor growth in vivo, as manifested by a significant decrease in the weight of the primary tumors. The enhancement of metastases is attributed to the suppression of cell-mediated immunity by the drug, but the mechanism of tumor-growth inhibition is not yet clear.", "PMID": 975080}
{"id": "pubmed23n0032_10151", "title": "Preferential inhibition of DNA synthesis in mouse hemopoietic cells by halothane.", "content": "The effect of prolonged light halothane anesthesia (0.8%) on the proliferation rate of different mouse tissues was investigated, using [5-125I]5-iodo-2-deoxyuridine uptake into DNA as the test parameter. It was found that DNA synthesis in spleen, femoral bone marrow, and, occasionally, the small intestine was significantly depressed after exposure for 24 hr to halothane in vivo. The time course of DNA synthesis inhibition was then investigated by utilizing a shorter (6-hr) exposure time. This period was found to be insufficient to cause DNA synthesis inhibition in any of test tissues. Because anesthesia was found to be associated with hypothermia at normal room temperatures, it was established that the inhibition of DNA synthesis was not due to cooling of the mice under anesthesia by demonstrating that inhibition in sensitive tissues occurred at warmer temperatures as well. To examine the specificity of this finding, the DNA synthesis rate of cells in other normal tissues, e.g., skin and muscle, and in s.c.-growing tumor cells of a mouse mammary carcinoma, L1210 leukemia, and a first transplant AKR lymphoma were examined. In none were responses noted with 24 hr of halothane exposure. However, halothane was found to inhibit DNA synthesis in regenerating marrow. Finally, it was found that after significant exposure to halothane, complete recovery was seen in the spleen after 24 hr, whereas femur DNA synthesis was still depressed by 20% at the same time.", "contents": "Preferential inhibition of DNA synthesis in mouse hemopoietic cells by halothane. The effect of prolonged light halothane anesthesia (0.8%) on the proliferation rate of different mouse tissues was investigated, using [5-125I]5-iodo-2-deoxyuridine uptake into DNA as the test parameter. It was found that DNA synthesis in spleen, femoral bone marrow, and, occasionally, the small intestine was significantly depressed after exposure for 24 hr to halothane in vivo. The time course of DNA synthesis inhibition was then investigated by utilizing a shorter (6-hr) exposure time. This period was found to be insufficient to cause DNA synthesis inhibition in any of test tissues. Because anesthesia was found to be associated with hypothermia at normal room temperatures, it was established that the inhibition of DNA synthesis was not due to cooling of the mice under anesthesia by demonstrating that inhibition in sensitive tissues occurred at warmer temperatures as well. To examine the specificity of this finding, the DNA synthesis rate of cells in other normal tissues, e.g., skin and muscle, and in s.c.-growing tumor cells of a mouse mammary carcinoma, L1210 leukemia, and a first transplant AKR lymphoma were examined. In none were responses noted with 24 hr of halothane exposure. However, halothane was found to inhibit DNA synthesis in regenerating marrow. Finally, it was found that after significant exposure to halothane, complete recovery was seen in the spleen after 24 hr, whereas femur DNA synthesis was still depressed by 20% at the same time.", "PMID": 975081}
{"id": "pubmed23n0032_10152", "title": "Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity.", "content": "This report describes the selection and behavior of tumor cells resistant to cytolysis by syngeneic lymphocytes. Two B16 melanoma lines, F1 (low metastasis) and F10 (high metastasis), were cultured with lymphocytes from C57BL/6 mice immunized against B16. The  selection procedure involved repeated exposure of the tumor cells to lymphocytes in vitro. After each interaction, the viable tumor cells were trpsinized, replated, and designated lines F1Lr-1 and F10Lr-1. The procedure was repeated five times, yielding lines F1Lr-6 and F10Lr-6, which resisted cytolysis by syngeneic lymphocytes. Mice were given s.c. or i.v. injections of cells from lines F1, F1Lr-6, F10, or F10Lr-6. Tumor growth patterns were the same for all four lines when the cells were injected s.c., however, the incidence of pulmonary metastases differed significantly after i.v. injection. Line F10 cells yielded more pulmonary metastases than an equal number of line F1 cells (p less than 0.01). F1Lr-6 cells yielded significantly fewer metastases than an equal number of lines F1 cells (p less than 0.01). A similar difference between F10Lr-6 and F10 cells was observed. The incidence of artificial metastases after i.v. injection of F10Lr-6 cells was similar to that for F1 cells. The quantitative organ distribution, arrest, and survival of i.v.-injected tumor cells were studied by using [125]-5-iodo-2'-deoxyuridinelabeled cells. There was a significantly greater number of cells from line F10, arrested and able to survive for 14 days in lungs, than cells from line F1. In contrast, cells from either line F1Lr-6 or F10Lr-6 had a lower incidence of arrest and survival than their lymphocyte-sensitive counterparts.", "contents": "Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity. This report describes the selection and behavior of tumor cells resistant to cytolysis by syngeneic lymphocytes. Two B16 melanoma lines, F1 (low metastasis) and F10 (high metastasis), were cultured with lymphocytes from C57BL/6 mice immunized against B16. The  selection procedure involved repeated exposure of the tumor cells to lymphocytes in vitro. After each interaction, the viable tumor cells were trpsinized, replated, and designated lines F1Lr-1 and F10Lr-1. The procedure was repeated five times, yielding lines F1Lr-6 and F10Lr-6, which resisted cytolysis by syngeneic lymphocytes. Mice were given s.c. or i.v. injections of cells from lines F1, F1Lr-6, F10, or F10Lr-6. Tumor growth patterns were the same for all four lines when the cells were injected s.c., however, the incidence of pulmonary metastases differed significantly after i.v. injection. Line F10 cells yielded more pulmonary metastases than an equal number of line F1 cells (p less than 0.01). F1Lr-6 cells yielded significantly fewer metastases than an equal number of lines F1 cells (p less than 0.01). A similar difference between F10Lr-6 and F10 cells was observed. The incidence of artificial metastases after i.v. injection of F10Lr-6 cells was similar to that for F1 cells. The quantitative organ distribution, arrest, and survival of i.v.-injected tumor cells were studied by using [125]-5-iodo-2'-deoxyuridinelabeled cells. There was a significantly greater number of cells from line F10, arrested and able to survive for 14 days in lungs, than cells from line F1. In contrast, cells from either line F1Lr-6 or F10Lr-6 had a lower incidence of arrest and survival than their lymphocyte-sensitive counterparts.", "PMID": 975082}
{"id": "pubmed23n0032_10153", "title": "Effects of Dintrato(1,2-diaminocyclohexane)platinum (NSC 239851) On murine myeloma and hemopoietic precursor cells.", "content": "We studied the effects of dinitrato (1,2,-diaminocyclohexane)platinum (NSC 239851) on murine myeloma  Adj. PC-5 and hemopoietic precursor cells. The median survival time of tumor-bearing mice was significantly affected by i.p. injections of this agent. While 14 mg/kg was a toxic dose, as little as 2 mg/kg prolonged the survival for over 100 days. When cells from normal marrow and actively regenerating marrows were exposed to the agent in culture tubes, washed, and assayed for the surviving hemopoietic precursors, similar sensitivity curves were observed. This result indicates the absence of cell cycle dependency of the agent. When marrow cells were exposed to NSC 239851 at 4 degrees in culture, almost total abrogation of the cytotoxicity was noted. This observation, unlike that from the experiment with the inorganic platinum congener, cis-diamminedichloroplatinum, suggests that the transport of this agents is an active process.", "contents": "Effects of Dintrato(1,2-diaminocyclohexane)platinum (NSC 239851) On murine myeloma and hemopoietic precursor cells. We studied the effects of dinitrato (1,2,-diaminocyclohexane)platinum (NSC 239851) on murine myeloma  Adj. PC-5 and hemopoietic precursor cells. The median survival time of tumor-bearing mice was significantly affected by i.p. injections of this agent. While 14 mg/kg was a toxic dose, as little as 2 mg/kg prolonged the survival for over 100 days. When cells from normal marrow and actively regenerating marrows were exposed to the agent in culture tubes, washed, and assayed for the surviving hemopoietic precursors, similar sensitivity curves were observed. This result indicates the absence of cell cycle dependency of the agent. When marrow cells were exposed to NSC 239851 at 4 degrees in culture, almost total abrogation of the cytotoxicity was noted. This observation, unlike that from the experiment with the inorganic platinum congener, cis-diamminedichloroplatinum, suggests that the transport of this agents is an active process.", "PMID": 975083}
{"id": "pubmed23n0032_10154", "title": "Homocysteine thiolactone metabolism in malignant cells.", "content": "Since abnormal homocysteine metabolism is associated with several disorders of growth, including neoplasia, the metabolic fate of homocysteine thiolactone was studied in cell cultures from several malignant tumors, established cell lines, cell lines transformed by oncogenic viruses, and normal skin cells. In all of the cultures of malignant cells homocysteine thiolactone becaome incorporated in peptide linkage with cellular proteins (thiolation). Normal cells incorporated homocysteine thiolactone in peptide linkage, but homocysteine thiolactone was released by acid hydrolysis. The findings suggest the speculative possibility that malignant cells are deficient in a homocysteine thiolactone derivative that prevents thiolation of proteins by homocysteine thiolactone. This hypothetical substance may also catalyze the synthesis of methionine and release acrolein, a growth-regulatory substance, in normal cells. The growth characteristics and tumorigenicity of cultured cells may be related to depletion of the hypothetical substance, and its identification, synthesis, and administration to animals would be expected to affect growth of malignant neoplasms.", "contents": "Homocysteine thiolactone metabolism in malignant cells. Since abnormal homocysteine metabolism is associated with several disorders of growth, including neoplasia, the metabolic fate of homocysteine thiolactone was studied in cell cultures from several malignant tumors, established cell lines, cell lines transformed by oncogenic viruses, and normal skin cells. In all of the cultures of malignant cells homocysteine thiolactone becaome incorporated in peptide linkage with cellular proteins (thiolation). Normal cells incorporated homocysteine thiolactone in peptide linkage, but homocysteine thiolactone was released by acid hydrolysis. The findings suggest the speculative possibility that malignant cells are deficient in a homocysteine thiolactone derivative that prevents thiolation of proteins by homocysteine thiolactone. This hypothetical substance may also catalyze the synthesis of methionine and release acrolein, a growth-regulatory substance, in normal cells. The growth characteristics and tumorigenicity of cultured cells may be related to depletion of the hypothetical substance, and its identification, synthesis, and administration to animals would be expected to affect growth of malignant neoplasms.", "PMID": 975084}
{"id": "pubmed23n0032_10155", "title": "The effect of adriamycin and radiation on G2 progression.", "content": "The effect of the DNA-intercalating antibiotic adriamycin on the progression of Chinese hamster ovary cells into mitosis, and on the delay induced by ionizing radiation, was studied using the mitotic cell selection procedure to monitor the rate of cell division. Following the addition of adriamycin, the mitotic rate remained unaltered for a refractory period and then decreased to zero. This effect was concentration dependent with transition points between the S-G2 boundary for 0.1 mug/ml and late G2 for 250 mug/ml. Cells treated with either a 10- or 30-min pulse of 1.0 mug adriamycin per ml exhibited a refractory period identical to that observed for continuous treatment. However, after a delay of congruent to 3.5 or congruent to 5 hr, respectively, cell division resumed. The mitotic rate of cells that received 150 rads of X-ray at the oneset of an adriamycin pulse declined coincident with that of radiation only, but resumed coincident with those receiving adriamycin only. This implies that radiation-induced division delay (congruent to 3 hr) was repaired before cells recovered from adriamycin-induced division delay and that the two agents were not additive. This lack of synergism is in contrast to that observed for cell lethality.", "contents": "The effect of adriamycin and radiation on G2 progression. The effect of the DNA-intercalating antibiotic adriamycin on the progression of Chinese hamster ovary cells into mitosis, and on the delay induced by ionizing radiation, was studied using the mitotic cell selection procedure to monitor the rate of cell division. Following the addition of adriamycin, the mitotic rate remained unaltered for a refractory period and then decreased to zero. This effect was concentration dependent with transition points between the S-G2 boundary for 0.1 mug/ml and late G2 for 250 mug/ml. Cells treated with either a 10- or 30-min pulse of 1.0 mug adriamycin per ml exhibited a refractory period identical to that observed for continuous treatment. However, after a delay of congruent to 3.5 or congruent to 5 hr, respectively, cell division resumed. The mitotic rate of cells that received 150 rads of X-ray at the oneset of an adriamycin pulse declined coincident with that of radiation only, but resumed coincident with those receiving adriamycin only. This implies that radiation-induced division delay (congruent to 3 hr) was repaired before cells recovered from adriamycin-induced division delay and that the two agents were not additive. This lack of synergism is in contrast to that observed for cell lethality.", "PMID": 975085}
{"id": "pubmed23n0032_10156", "title": "Decreased prevalence of immediate hypersensitivity (atopy) in a cancer population.", "content": "It has been suggested that the atopic population has decreased risk of cancer. This investigation examined the cumulative prevalence of atopy in a population with neoplastic disease and compared this with the prevalence of atopy in an age-matched control group and with published estimates of atopy in the general peopulation. Seventy-four patients with neoplastic disease and 86 patients without cancer were evaluated. The subjects were given a standard allergic questionnaire which evaluated them with regard to a history of allergic symptoms, hives, eczema, frequent colds, frequent unexplained rashes, hay fever, and asthma. All were skin tested with a representative group of regionally significant allergens. There was a 15-fold decrease in prevalence of atopy in the cancer population, compared with the control group and compared with published estimates of atopy in the general population.", "contents": "Decreased prevalence of immediate hypersensitivity (atopy) in a cancer population. It has been suggested that the atopic population has decreased risk of cancer. This investigation examined the cumulative prevalence of atopy in a population with neoplastic disease and compared this with the prevalence of atopy in an age-matched control group and with published estimates of atopy in the general peopulation. Seventy-four patients with neoplastic disease and 86 patients without cancer were evaluated. The subjects were given a standard allergic questionnaire which evaluated them with regard to a history of allergic symptoms, hives, eczema, frequent colds, frequent unexplained rashes, hay fever, and asthma. All were skin tested with a representative group of regionally significant allergens. There was a 15-fold decrease in prevalence of atopy in the cancer population, compared with the control group and compared with published estimates of atopy in the general population.", "PMID": 975086}
{"id": "pubmed23n0032_10157", "title": "Identification and characterization of a circulating tumor-associated oncofetal protein from a radiation-induced adenocarcinoma of the rat small bowel.", "content": "A tumor-associated protein from the cellular membranes of a radiation-induced rat small bowel adenocarcinoma was identified, found to be serologically unaltered in the circulatory system, and was observed to be susceptible to acid hydrolysis. The immunochemical reactivity was unchanged by heat, alkali, or neuraminidase digestion. The protein appeared to be a single immunologically active species, but it was structurally composed of a heterogeneous group of proteins.", "contents": "Identification and characterization of a circulating tumor-associated oncofetal protein from a radiation-induced adenocarcinoma of the rat small bowel. A tumor-associated protein from the cellular membranes of a radiation-induced rat small bowel adenocarcinoma was identified, found to be serologically unaltered in the circulatory system, and was observed to be susceptible to acid hydrolysis. The immunochemical reactivity was unchanged by heat, alkali, or neuraminidase digestion. The protein appeared to be a single immunologically active species, but it was structurally composed of a heterogeneous group of proteins.", "PMID": 975087}
{"id": "pubmed23n0032_10158", "title": "Growth stimulation of cultured endothelial cells by tumor cell homogenates.", "content": "Short-term cultures of endothelial cells from fetal bovine heart and aorta consistently display increased growth rates when crude tumor cell homogenates from the Walker 256 adenocarcinoma (ascites and solid forms as well as tumor cells from a suspension culture) are added to the culture media. The tumor-derived material produces growth stimulation in both sparse and confluient endothelial cell cultures. Homogenates of embryonic tissues and cultured cells show similar growth-promoting effects; corresponding material from various adult tissues is ineffective. The nonendothelial cells that were tested generally show no growth response or only a slight positive growth response to the tumor cell homogenates. The results indicate the feasibility of using this in vitro system as a paradigm of the tumor-induced vascularization process.", "contents": "Growth stimulation of cultured endothelial cells by tumor cell homogenates. Short-term cultures of endothelial cells from fetal bovine heart and aorta consistently display increased growth rates when crude tumor cell homogenates from the Walker 256 adenocarcinoma (ascites and solid forms as well as tumor cells from a suspension culture) are added to the culture media. The tumor-derived material produces growth stimulation in both sparse and confluient endothelial cell cultures. Homogenates of embryonic tissues and cultured cells show similar growth-promoting effects; corresponding material from various adult tissues is ineffective. The nonendothelial cells that were tested generally show no growth response or only a slight positive growth response to the tumor cell homogenates. The results indicate the feasibility of using this in vitro system as a paradigm of the tumor-induced vascularization process.", "PMID": 975088}
{"id": "pubmed23n0032_10159", "title": "A rapid in vitro labeling index method for predicting response of human solid tumors to chemotherapy.", "content": "A rapid autoradiographic technique for measuring the [3H]thymidine-labeling index of human solid tumors has been adapted to assess the effect of anticancer drugs in vitro. The drugs tested were present unchanged or as metabolites in serum obtained from patients immediately post-treatment. In 15 patients, the drugs tested in vitro were also given in vivo. Tumor-labeling index fell significantly in 5 of 6 patients who were later found to have objective clinical response. Tumor-labeling index did not change significantly in 8 patients and rose significantly in 1 of 9 patients who lacked clinical response. If confirmed, this in vitro test may prove to be a useful method of predicting responsiveness of human solid cancers to chemotherapeutic agents.", "contents": "A rapid in vitro labeling index method for predicting response of human solid tumors to chemotherapy. A rapid autoradiographic technique for measuring the [3H]thymidine-labeling index of human solid tumors has been adapted to assess the effect of anticancer drugs in vitro. The drugs tested were present unchanged or as metabolites in serum obtained from patients immediately post-treatment. In 15 patients, the drugs tested in vitro were also given in vivo. Tumor-labeling index fell significantly in 5 of 6 patients who were later found to have objective clinical response. Tumor-labeling index did not change significantly in 8 patients and rose significantly in 1 of 9 patients who lacked clinical response. If confirmed, this in vitro test may prove to be a useful method of predicting responsiveness of human solid cancers to chemotherapeutic agents.", "PMID": 975089}
{"id": "pubmed23n0032_10160", "title": "Effects of 5-mercapto-2'-deoxyuridine on the incorporation of nucleosides into RNA and DNA in a primary lymphocyte culture system.", "content": "The effects of 5-mercapto-2'-deoxyuridine (MUdr) on DNA synthesis in a primary murine spleen lymphocyte culture system stimulated by phytohemagglutinin (PHA) were studied. Inhibition of thymidine incorporation into acid insoluble nucleic acid material was 50% at 0.5 mM MUdR concentration, while inhibition of deoxyuridine incorporation into acid-insoluble nucleic acids was 50% at 0.01 mM MUdR. Time course studies, at 0.5 and 0.05 mM MUdR, showed that the magnitude of inhibition of incorporation for thymidine and deoxyuridine, respectively, increased from a time point after PHA stimulation when increased synthesis of thymidine kinase and thymidylate synthetase had leveled off. At 1 mM MUdR, total cellular DNA in cultures was decreased 43% at 42 hr after PHA stimulation. Neither the total number of cells nor the percentage of PHA-transformed cells was decreased in comparison to that of controls. MUdR therefore blocks the increase in DNA content of lymphocytes that is initiated during the S phase of the cell cycle. Millimolar levels of MUdR inhibited incorporation or uridine, adenosine, and cytidine into acid-insoluble material in pha-stimulated primary murine lymphocyte cultures. Total cellular RNA synthesis was inhibited at these levels of MUdR, with no differential effects on 4, 18, or 28 S RNA species observed. Uptake of these nucleosides into the total cellular acid-soluble material was not blocked. Uptake of different labeled nucleosides into cellular, acid-soluble pools occurs at different rates. Thus, choice of a suitable minimum pulse time to achieve saturation for different labeled nucleosides must relate to this consideration. Thymidine kinase from whole-cell sonic extracts of PHA-stimulated lymphocytes was inhibited 65% by 1 mM MUdR at 24 and 48 hr after stimulation. Uridine kinase extracted from the PHA-stimulated cells was also significantly inhibited by 1mM MUdR at 24 hr (56%). Exogenous guanosine incorporation into lympohcyte acid-insoluble material is increased by MUdR. This increased utilization of exogenous nuceloside is apparently the result of MUdR inhibition of conversion of adenosine to guanine nucleotides within the lymphocytes and a consequent diminution of the total intracellular guanine nucleotide pool size. The active inhibitory compound is the deoxyribonucleoside or deoxyribonucleotide. Comparison with the riboside analog 5-mercaptouridine showed that MUdR was a more efficient inhibitor of nucleoside incorporation.", "contents": "Effects of 5-mercapto-2'-deoxyuridine on the incorporation of nucleosides into RNA and DNA in a primary lymphocyte culture system. The effects of 5-mercapto-2'-deoxyuridine (MUdr) on DNA synthesis in a primary murine spleen lymphocyte culture system stimulated by phytohemagglutinin (PHA) were studied. Inhibition of thymidine incorporation into acid insoluble nucleic acid material was 50% at 0.5 mM MUdR concentration, while inhibition of deoxyuridine incorporation into acid-insoluble nucleic acids was 50% at 0.01 mM MUdR. Time course studies, at 0.5 and 0.05 mM MUdR, showed that the magnitude of inhibition of incorporation for thymidine and deoxyuridine, respectively, increased from a time point after PHA stimulation when increased synthesis of thymidine kinase and thymidylate synthetase had leveled off. At 1 mM MUdR, total cellular DNA in cultures was decreased 43% at 42 hr after PHA stimulation. Neither the total number of cells nor the percentage of PHA-transformed cells was decreased in comparison to that of controls. MUdR therefore blocks the increase in DNA content of lymphocytes that is initiated during the S phase of the cell cycle. Millimolar levels of MUdR inhibited incorporation or uridine, adenosine, and cytidine into acid-insoluble material in pha-stimulated primary murine lymphocyte cultures. Total cellular RNA synthesis was inhibited at these levels of MUdR, with no differential effects on 4, 18, or 28 S RNA species observed. Uptake of these nucleosides into the total cellular acid-soluble material was not blocked. Uptake of different labeled nucleosides into cellular, acid-soluble pools occurs at different rates. Thus, choice of a suitable minimum pulse time to achieve saturation for different labeled nucleosides must relate to this consideration. Thymidine kinase from whole-cell sonic extracts of PHA-stimulated lymphocytes was inhibited 65% by 1 mM MUdR at 24 and 48 hr after stimulation. Uridine kinase extracted from the PHA-stimulated cells was also significantly inhibited by 1mM MUdR at 24 hr (56%). Exogenous guanosine incorporation into lympohcyte acid-insoluble material is increased by MUdR. This increased utilization of exogenous nuceloside is apparently the result of MUdR inhibition of conversion of adenosine to guanine nucleotides within the lymphocytes and a consequent diminution of the total intracellular guanine nucleotide pool size. The active inhibitory compound is the deoxyribonucleoside or deoxyribonucleotide. Comparison with the riboside analog 5-mercaptouridine showed that MUdR was a more efficient inhibitor of nucleoside incorporation.", "PMID": 975090}
{"id": "pubmed23n0032_10161", "title": "Relationship of cell growth behavior in vitro to tumorigenicity in athymic nude mice.", "content": "The serum requirements, anchorage requirements, saturation densities, and contact inhibition responses of a variety of mammalian cell lines were determined under uniform conditions. The serum requirement of both transformed and normal cells was a sensitive function of initial plating density. Cloning efficiency on irradiated mouse monolayers was found to be an invalid indicator of contact inhibition of growth, since most cell lines that failed to form visible colonies on cell monolayers nonetheless proliferated on these monolayers. When normal and neoplastic cells from a variety of sources were examined, none of the growth parameters that serve to define the transformed state in vitro correlated consistently with cellular tumorigenicity in athymic nude mice. It is concluded that the most reliable and physiologically meaningful assay for malignant transformation is, at present, cellular tumorigenicity in athymic nude mice.", "contents": "Relationship of cell growth behavior in vitro to tumorigenicity in athymic nude mice. The serum requirements, anchorage requirements, saturation densities, and contact inhibition responses of a variety of mammalian cell lines were determined under uniform conditions. The serum requirement of both transformed and normal cells was a sensitive function of initial plating density. Cloning efficiency on irradiated mouse monolayers was found to be an invalid indicator of contact inhibition of growth, since most cell lines that failed to form visible colonies on cell monolayers nonetheless proliferated on these monolayers. When normal and neoplastic cells from a variety of sources were examined, none of the growth parameters that serve to define the transformed state in vitro correlated consistently with cellular tumorigenicity in athymic nude mice. It is concluded that the most reliable and physiologically meaningful assay for malignant transformation is, at present, cellular tumorigenicity in athymic nude mice.", "PMID": 975091}
{"id": "pubmed23n0032_10162", "title": "Changes in ornithine decarboxylase activity in rat intestines during aging.", "content": "The ornithine decarboxylase (ODC) activity of rat intestines, liver, and brain was found to vary dramatically as animals develop and age. Unusually high activity was present in the small intestines of adult animals. The ODC activity of the small intestines approximated that of fetal tissues and of regenerating rat liver. Putrescine, spermine, and spermidine levels of fetal and adult animal tissues were determined. In all tissues but the stomach mucosa, high putrescine levels correlated with high IDC activity. However, the total polyamine concentrations of the stomach and colon could not be correlated with ODC levels, and no simple relationship between polyamine levels of ODC levels and cellular proliferation in the gut was found.", "contents": "Changes in ornithine decarboxylase activity in rat intestines during aging. The ornithine decarboxylase (ODC) activity of rat intestines, liver, and brain was found to vary dramatically as animals develop and age. Unusually high activity was present in the small intestines of adult animals. The ODC activity of the small intestines approximated that of fetal tissues and of regenerating rat liver. Putrescine, spermine, and spermidine levels of fetal and adult animal tissues were determined. In all tissues but the stomach mucosa, high putrescine levels correlated with high IDC activity. However, the total polyamine concentrations of the stomach and colon could not be correlated with ODC levels, and no simple relationship between polyamine levels of ODC levels and cellular proliferation in the gut was found.", "PMID": 975092}
{"id": "pubmed23n0032_10163", "title": "Detection of occult metastatic melanoma by urine chromatography.", "content": "By using ion-exchange column chromatography with effluent monitoring using the stable, free radical alpha,alpha-diphenyl-beta-picryhydrazyl as a colorimetric reagent, we have demonstrated the occurrence of elevated levels of five peaks in the urine of patients with metastatic disease. The tentative assignment of two of the peaks as 3,4-dihydroxyphenylalanine and as 3-methoxy-4-hydroxyphenylalanine has been made. Three remain unknown. The correlation of these peaks with the clinical status of melanoma patients shows that, while the individual excretion pattern of these compounds may be variable, the sustained occurrence of one or more of them in a patient's urine is evidence of recurrent or continuing disease. The excretion levels appear to be proportional to the tumor burden. The results with a group of 39 melanoma patientshaving Stage II or Stage III disease indicate that this chromatography technique provides earlier evidenc eof liver metastases than doses the liver scan, may detect occult metastases generally, and has detected tumor in clinically enlarged lymph nodes. This method, in its present form, does not detect small pulmonary lesions earlier than chest X-ray or tomography do or brain metastases earlier than do brain scan or computerized axial tomography. The technique is clinically useful for the diagnosis of melanoma patients and in their follow-up while under treatment.", "contents": "Detection of occult metastatic melanoma by urine chromatography. By using ion-exchange column chromatography with effluent monitoring using the stable, free radical alpha,alpha-diphenyl-beta-picryhydrazyl as a colorimetric reagent, we have demonstrated the occurrence of elevated levels of five peaks in the urine of patients with metastatic disease. The tentative assignment of two of the peaks as 3,4-dihydroxyphenylalanine and as 3-methoxy-4-hydroxyphenylalanine has been made. Three remain unknown. The correlation of these peaks with the clinical status of melanoma patients shows that, while the individual excretion pattern of these compounds may be variable, the sustained occurrence of one or more of them in a patient's urine is evidence of recurrent or continuing disease. The excretion levels appear to be proportional to the tumor burden. The results with a group of 39 melanoma patientshaving Stage II or Stage III disease indicate that this chromatography technique provides earlier evidenc eof liver metastases than doses the liver scan, may detect occult metastases generally, and has detected tumor in clinically enlarged lymph nodes. This method, in its present form, does not detect small pulmonary lesions earlier than chest X-ray or tomography do or brain metastases earlier than do brain scan or computerized axial tomography. The technique is clinically useful for the diagnosis of melanoma patients and in their follow-up while under treatment.", "PMID": 975093}
{"id": "pubmed23n0032_10164", "title": "Transfer RNA species in tumors of different growth rates.", "content": "Tyrosyl-, histidyl-, lysyl-, and phenylalanyl-tRNA's from 3 tumors (DBAH, DBAH, and DBA), differing in growth rates and from host mammary glands and liver, were compared by means of methylated albumin kieselguhr (MAK) column and by reverse-phase-5 chromatography. The elution profiles of lysyl-tRNA's from DBAH and DBA, phenylalanyl Trna's from DBAH and DBAH, and histidyl-tRNA's from DBA3 tumors exhibited extra isoaccepting species, compared with host liver and mammary glands. The distribution of acylatable tyrosyl-tRNA's in DBA3 and DBAH, phenylalanyl-tRNA's in DBAH and DBAH2, and histid-l-tRNA's in DBA3 is higher than that in liver, whereas no appreciable differences were observed in the lysyl-tRNA contents of the tumors and liver. The chromatographic alternations appeared to be a property of the tumor tRNA's and not due to differences in aminoacyl-tRNA synthetases or due to the aggregation of tRNA's. The structural and functional significance of these findings are discussed.", "contents": "Transfer RNA species in tumors of different growth rates. Tyrosyl-, histidyl-, lysyl-, and phenylalanyl-tRNA's from 3 tumors (DBAH, DBAH, and DBA), differing in growth rates and from host mammary glands and liver, were compared by means of methylated albumin kieselguhr (MAK) column and by reverse-phase-5 chromatography. The elution profiles of lysyl-tRNA's from DBAH and DBA, phenylalanyl Trna's from DBAH and DBAH, and histidyl-tRNA's from DBA3 tumors exhibited extra isoaccepting species, compared with host liver and mammary glands. The distribution of acylatable tyrosyl-tRNA's in DBA3 and DBAH, phenylalanyl-tRNA's in DBAH and DBAH2, and histid-l-tRNA's in DBA3 is higher than that in liver, whereas no appreciable differences were observed in the lysyl-tRNA contents of the tumors and liver. The chromatographic alternations appeared to be a property of the tumor tRNA's and not due to differences in aminoacyl-tRNA synthetases or due to the aggregation of tRNA's. The structural and functional significance of these findings are discussed.", "PMID": 975094}
{"id": "pubmed23n0032_10165", "title": "Characterization of dextran sulfate-treated ascites tumor cells and their repair by ascites fluid.", "content": "Exposure of Ehrlich ascites tumor cells to the polyanion dextran sulfate altered the permeability of the plasma membrane resulting in decreased glycolysis and respiration, enhanced permeability to Rb+ and adenosine 5'-triphosphate, and increased staining by erythrosin B. The inhibition of respiration was reversed by the addition of inorganic phosphate, whereas restoration of glycolysis required both inorganic phosphate and adenosine 5'-monophosphate. The membrane lesion was repaired by injection of dextran sulfate-treated cells into mice or by in vitro exposure to a heat-stable extract from ascites fluid. Reparied cells were no longer stained by erythrosin B and glycolyzed without added adenosine 5'-monophosphate. The time course of repaire was separable into a rapid initial repair of the membrane lesion followed by a slower recovery of the normal intracellular ionic composition. Both phases were insensitive to inhibitors of protein synthesis. The repair activity of ascites fluid was heat stable and was composed of both dialyzable and nondialyzable factors. Incubation of dextran sulfate-treated cells with ascites fluid restored Rb+ uptake and abolished the adenosine 5'-triphosphate-stimulated Ca2+ uptake.", "contents": "Characterization of dextran sulfate-treated ascites tumor cells and their repair by ascites fluid. Exposure of Ehrlich ascites tumor cells to the polyanion dextran sulfate altered the permeability of the plasma membrane resulting in decreased glycolysis and respiration, enhanced permeability to Rb+ and adenosine 5'-triphosphate, and increased staining by erythrosin B. The inhibition of respiration was reversed by the addition of inorganic phosphate, whereas restoration of glycolysis required both inorganic phosphate and adenosine 5'-monophosphate. The membrane lesion was repaired by injection of dextran sulfate-treated cells into mice or by in vitro exposure to a heat-stable extract from ascites fluid. Reparied cells were no longer stained by erythrosin B and glycolyzed without added adenosine 5'-monophosphate. The time course of repaire was separable into a rapid initial repair of the membrane lesion followed by a slower recovery of the normal intracellular ionic composition. Both phases were insensitive to inhibitors of protein synthesis. The repair activity of ascites fluid was heat stable and was composed of both dialyzable and nondialyzable factors. Incubation of dextran sulfate-treated cells with ascites fluid restored Rb+ uptake and abolished the adenosine 5'-triphosphate-stimulated Ca2+ uptake.", "PMID": 975095}
{"id": "pubmed23n0032_10166", "title": "Effect of deoxyribonuclease on adriamycin-polynucleotide complexes.", "content": "Recent interest in the use of adriamycin-DNA complex as an approach to improve the therapeutic effectiveness and to reduce toxicity of adriamycin for cancer chemotherapy requires an in-depth understanding of the physicochemical and biochemical properties of such complexes. The interactions of adriamycin with single-strand polydeoxyribonucleotides, double-strand DNA, and double-strand ribodeoxyribopolynucleotide hybrids were therfore investigated. Association constants (Kapp) of adriamycin and polynucleotides were obtained. These data showed that the inherent variable in such complex lies in the composition of the polynucleotides. Alternate deoxyguanylate (dG)-deoxycytidylate (dC) sequence binds 7-fold better than alternate deoxyadenylate (dA)-deoxythymidylate (dT) sequence. Comparative studies of the hydrolysis of DNA duplexes by deoxyribonucleases I and II with and without adriamycin were also carried out. The rate of hydrolysis decreased in the order poly(dA-dT) greater than calf thymus DNA greater than poly(dG-dC) greater than poly(dA)-poly(dT) greater than poly(dG)-poly(dC) for DNase I and poly(dA)-dT) greater than calf thymus DNA greater than poly(dG-dC) greater than poly(dA)-poly(dT) greater than poly(dG)-poly(dC) for DNase II. Intercalation of adriamycin to deoxyribopolynucleotide duplex resulted in inhibition of DNase II two to three times more than tat of DNase I. On the other hand, intercalation of adriamycin to homodeoxypolynucleotide duplex poly(dA)-poly(dT) and poly(dG)-poly(dC) enhanced the DNase I hydrolysis. If DNase I activity could be related to serum DNase and DNase II related to tumor lyososomal DNase as in the endocytosis mechanism proposed by Trouet et al. (Cancer Chemotherapy Rept., 59: 260, 1975), the best adriamycin carrier suggested by this investigation could be poly(dA)-poly(dT) and poly(dG-dC). It is also suggested in this study that adriamycin-RNA-DNA hybrid could be of interest as an antiviral agent by a similar release mechanism via RNase H, an enzyme associated with viral reverse transcriptase.", "contents": "Effect of deoxyribonuclease on adriamycin-polynucleotide complexes. Recent interest in the use of adriamycin-DNA complex as an approach to improve the therapeutic effectiveness and to reduce toxicity of adriamycin for cancer chemotherapy requires an in-depth understanding of the physicochemical and biochemical properties of such complexes. The interactions of adriamycin with single-strand polydeoxyribonucleotides, double-strand DNA, and double-strand ribodeoxyribopolynucleotide hybrids were therfore investigated. Association constants (Kapp) of adriamycin and polynucleotides were obtained. These data showed that the inherent variable in such complex lies in the composition of the polynucleotides. Alternate deoxyguanylate (dG)-deoxycytidylate (dC) sequence binds 7-fold better than alternate deoxyadenylate (dA)-deoxythymidylate (dT) sequence. Comparative studies of the hydrolysis of DNA duplexes by deoxyribonucleases I and II with and without adriamycin were also carried out. The rate of hydrolysis decreased in the order poly(dA-dT) greater than calf thymus DNA greater than poly(dG-dC) greater than poly(dA)-poly(dT) greater than poly(dG)-poly(dC) for DNase I and poly(dA)-dT) greater than calf thymus DNA greater than poly(dG-dC) greater than poly(dA)-poly(dT) greater than poly(dG)-poly(dC) for DNase II. Intercalation of adriamycin to deoxyribopolynucleotide duplex resulted in inhibition of DNase II two to three times more than tat of DNase I. On the other hand, intercalation of adriamycin to homodeoxypolynucleotide duplex poly(dA)-poly(dT) and poly(dG)-poly(dC) enhanced the DNase I hydrolysis. If DNase I activity could be related to serum DNase and DNase II related to tumor lyososomal DNase as in the endocytosis mechanism proposed by Trouet et al. (Cancer Chemotherapy Rept., 59: 260, 1975), the best adriamycin carrier suggested by this investigation could be poly(dA)-poly(dT) and poly(dG-dC). It is also suggested in this study that adriamycin-RNA-DNA hybrid could be of interest as an antiviral agent by a similar release mechanism via RNase H, an enzyme associated with viral reverse transcriptase.", "PMID": 975096}
{"id": "pubmed23n0032_10167", "title": "Potential regulatory roles for RNA in cellular development.", "content": "In addition to its widely accepted messenger and structural roles, RNA has been implicated in several other biological events: transfer of specificity of the immune response, interferon induction, and infection of plants by viroids. In none of these cases is the mechanism of RNA action understood. As a result of recent advances in the understanding of RNA metabolism in eukaryotic nuclei and potential specificity of nucleases that cleave such RNA, we have suggested specific ways in which \"extra\" RNA could be involved in regulation of the development of cells. A survey of literature concerning mammalian cell differentiation both in embryos and in the immune system leads to the observation that there appears to be a line of communication between macromolecules on cell surfaces and the genome. Making the conservative assumption that the DNA retains its integrity throughout development, it seems likely that highly specific signals are sometimes required to transfer information from outside the cell to the DNA,resulting in a change in state of differentiation. We propose a way in which RNA could be utilized in this process.", "contents": "Potential regulatory roles for RNA in cellular development. In addition to its widely accepted messenger and structural roles, RNA has been implicated in several other biological events: transfer of specificity of the immune response, interferon induction, and infection of plants by viroids. In none of these cases is the mechanism of RNA action understood. As a result of recent advances in the understanding of RNA metabolism in eukaryotic nuclei and potential specificity of nucleases that cleave such RNA, we have suggested specific ways in which \"extra\" RNA could be involved in regulation of the development of cells. A survey of literature concerning mammalian cell differentiation both in embryos and in the immune system leads to the observation that there appears to be a line of communication between macromolecules on cell surfaces and the genome. Making the conservative assumption that the DNA retains its integrity throughout development, it seems likely that highly specific signals are sometimes required to transfer information from outside the cell to the DNA,resulting in a change in state of differentiation. We propose a way in which RNA could be utilized in this process.", "PMID": 975098}
{"id": "pubmed23n0032_10168", "title": "Selective control of DNA helix openings during gene regulation.", "content": "DNA helices must undergo openings in the form of localized strand separations in order to permit the onset of RNA synthesis or DNA synthesis. The selective control of such DNA helix openings at particular gene loci is the critical feature of gene regulation in prokaryotes and eukaryotes.", "contents": "Selective control of DNA helix openings during gene regulation. DNA helices must undergo openings in the form of localized strand separations in order to permit the onset of RNA synthesis or DNA synthesis. The selective control of such DNA helix openings at particular gene loci is the critical feature of gene regulation in prokaryotes and eukaryotes.", "PMID": 975099}
{"id": "pubmed23n0032_10169", "title": "Continuity of a rabbit antigen between generations.", "content": "The rabbit uterine protein, blastokinin, is detectable as an antigen in both male and female genital tract tissues, in preimplantation embryos, and in lungs from late fetal stages, immatures, and adults. Thus, it serves as an example of how an antigen of normal tissue exists in a developmental progression from one generation to the next.", "contents": "Continuity of a rabbit antigen between generations. The rabbit uterine protein, blastokinin, is detectable as an antigen in both male and female genital tract tissues, in preimplantation embryos, and in lungs from late fetal stages, immatures, and adults. Thus, it serves as an example of how an antigen of normal tissue exists in a developmental progression from one generation to the next.", "PMID": 975100}
{"id": "pubmed23n0032_10170", "title": "Activation of developmental genes in neoplastic transformation.", "content": "The view is advanced that it is important to fix the stage of development corresponding to the pattern of expression of oncodevelopmental proteins in neoplastic transformation and neoplasia. From a map of development focused on events beginning with the zygote and ending with the establishment of fetus and placenta, it is possible to explain why certain developmental gene products are restricted to fetus or placenta or distributed in both. It also suggests which products can be expected to be expressed concordantly in neoplastic transformation and in neoplasia.", "contents": "Activation of developmental genes in neoplastic transformation. The view is advanced that it is important to fix the stage of development corresponding to the pattern of expression of oncodevelopmental proteins in neoplastic transformation and neoplasia. From a map of development focused on events beginning with the zygote and ending with the establishment of fetus and placenta, it is possible to explain why certain developmental gene products are restricted to fetus or placenta or distributed in both. It also suggests which products can be expected to be expressed concordantly in neoplastic transformation and in neoplasia.", "PMID": 975101}
{"id": "pubmed23n0032_10171", "title": "Fetal growth modified by natural and induced changes in maternal reactivity.", "content": "Maternal immunological unresponsiveness has usually been demonstrated with adult tissue allografts. Experiments were first directed to the question of whether maternal reactivity to allogeneic conceptuses parallels that of implants of allogeneic tumor. BALB/c female mice parous by C3H males for one to eight litters and challenged with Tumor 70429 of C3h origin showed increasing unresponsiveness with multiparity. A high percentage of progressively growing tumors in highly multiparous females indicates that unresponsiveness does not give way to sensitization. The effects of multiparity on placental and fetal weights in BALB/c females pregnant by C3H males resulted in a continuing decrease in placental weight (indicative of unresponsiveness) through the third pregnancy, but this was followed by a contrasting progressive weight increase indicating sensitization with four or more pregnancises. Syngeneic placentas displayed a similar pattern suggesting that weight changes were not entirely alloantigen dependent and that fetus-specific antigens also alter maternal reactivity. The effects of specific and nonspecific maternal preimmunization and specific parity on placental weights were tested as follows. One group of BALB/c females was immunized against C3H (specific), a second was immunized against DBA/2 (nonspecific), and a third was not immunized. Each of these groups was divided in half and mated to either C3H or DBA/2 males for a first litter; then all were mated to C3H for a second pregnancy. Placental weights were significantly smaller in females that had their first litter by C3H males. A similar experiment with BALB/c X C57BL F1 females resulted in fewer changes in placental weights. In all instances, maternal reactivity had its major effect upon placental rather than fetal weight. The effect of maternal unresponsiveness upon reproductive capacity was tested by mating BALB/c females to either DBA/2 POR C3H males for a first litter and then mating all females ot C3H males for a second litter. Second litters were significantly larger when first litters were also sired by C3H males.", "contents": "Fetal growth modified by natural and induced changes in maternal reactivity. Maternal immunological unresponsiveness has usually been demonstrated with adult tissue allografts. Experiments were first directed to the question of whether maternal reactivity to allogeneic conceptuses parallels that of implants of allogeneic tumor. BALB/c female mice parous by C3H males for one to eight litters and challenged with Tumor 70429 of C3h origin showed increasing unresponsiveness with multiparity. A high percentage of progressively growing tumors in highly multiparous females indicates that unresponsiveness does not give way to sensitization. The effects of multiparity on placental and fetal weights in BALB/c females pregnant by C3H males resulted in a continuing decrease in placental weight (indicative of unresponsiveness) through the third pregnancy, but this was followed by a contrasting progressive weight increase indicating sensitization with four or more pregnancises. Syngeneic placentas displayed a similar pattern suggesting that weight changes were not entirely alloantigen dependent and that fetus-specific antigens also alter maternal reactivity. The effects of specific and nonspecific maternal preimmunization and specific parity on placental weights were tested as follows. One group of BALB/c females was immunized against C3H (specific), a second was immunized against DBA/2 (nonspecific), and a third was not immunized. Each of these groups was divided in half and mated to either C3H or DBA/2 males for a first litter; then all were mated to C3H for a second pregnancy. Placental weights were significantly smaller in females that had their first litter by C3H males. A similar experiment with BALB/c X C57BL F1 females resulted in fewer changes in placental weights. In all instances, maternal reactivity had its major effect upon placental rather than fetal weight. The effect of maternal unresponsiveness upon reproductive capacity was tested by mating BALB/c females to either DBA/2 POR C3H males for a first litter and then mating all females ot C3H males for a second litter. Second litters were significantly larger when first litters were also sired by C3H males.", "PMID": 975102}
{"id": "pubmed23n0032_10172", "title": "The significance of embryonic reexpression in cancer.", "content": "Irradiated syngeneic mouse testicular cells and the first-trimester human fetal tissue cells protect mice partially against tumors induced by methylcholanthrene or against a syngeneic transplantable mouse tumor, Meth A, respectively. For future research it is suggested that experiments should have a therapeutic rather than a prophylactic design and that the induction of tolerance to fetal antigens could be a highly informative procedure.", "contents": "The significance of embryonic reexpression in cancer. Irradiated syngeneic mouse testicular cells and the first-trimester human fetal tissue cells protect mice partially against tumors induced by methylcholanthrene or against a syngeneic transplantable mouse tumor, Meth A, respectively. For future research it is suggested that experiments should have a therapeutic rather than a prophylactic design and that the induction of tolerance to fetal antigens could be a highly informative procedure.", "PMID": 975103}
{"id": "pubmed23n0032_10173", "title": "Antigens associated with normal and malignant gastrointestinal tissues.", "content": "Immunization of hamsters with phenol-alcohol extracts of GW-39 human colonic tumor tissues has resulted in the identification of three gastrointestinal tissue-associated antigens, on the basis of precipitin immunoreactivity. Sephadex G-200 and Bio-Gel A-15m chromatography of normal colonic tissue and GW-39 tumor extracts revealed antigen immunoreactivity in the 46,000 (low-molecular-weight), 170,000 to 900,000 (high-molecular-weight), and 5 to 10 million (very high-molecular-weight) ranges or low-molecular-weight colon-specific antigen (LMW/CSA), high-molecular-weight colon-specific antigen (HMW/CSA), and very-high-molecular-weight colon-specific antigen (VHMW/CSA), respectively. Immunodifussion reactions indicated that the HMW/CSA was human gastrointestinal tissue-specific, increasing in concentration from the esophagus to the colon [for which reason the term colon-specific antigen (CSA) has been retained], whereas the LMW/CSA was found in human gastrointestinal tissues, hamster and rat colon, human saliva, and normal human cervix. Colon-specific antigen (CSA) could be demonstrated in human gastrointestinal tumors, including the LS-174T colonic cancer cell line, but not in cancers of other sites tested. Likewise, CSA's were found in fetal human gut tissue. Whereas HMW/CSA and VHMW/CSA showed partial identity in immunodiffusion, HMW/CSA and VHMW/CSA, as well as LMW/CSA and VHMW/CSA, showed distinct immunoprecipitin bands, respectively. The immunoelectrophoretic mobility of VHMW/CSA was similar to an alpha-globulin, whereas HMW/CSA and LMW/CSA migrated to the prealbumin region. CSA appeared in immunofluorescence of GW-39 tumor cells and in the goblet cells of human colon predominantly as a cell-surface component. Staining with periodic acid-Schiff and solubility characteristics of the CSA's suggest that they are glycoprotein in nature. These studies thus support the view that organ-specific and organ-associated antigens of the colon can be maintained and expressed in human colonic carcinomas, including the xenografted GW-39 human colonic tumor system.", "contents": "Antigens associated with normal and malignant gastrointestinal tissues. Immunization of hamsters with phenol-alcohol extracts of GW-39 human colonic tumor tissues has resulted in the identification of three gastrointestinal tissue-associated antigens, on the basis of precipitin immunoreactivity. Sephadex G-200 and Bio-Gel A-15m chromatography of normal colonic tissue and GW-39 tumor extracts revealed antigen immunoreactivity in the 46,000 (low-molecular-weight), 170,000 to 900,000 (high-molecular-weight), and 5 to 10 million (very high-molecular-weight) ranges or low-molecular-weight colon-specific antigen (LMW/CSA), high-molecular-weight colon-specific antigen (HMW/CSA), and very-high-molecular-weight colon-specific antigen (VHMW/CSA), respectively. Immunodifussion reactions indicated that the HMW/CSA was human gastrointestinal tissue-specific, increasing in concentration from the esophagus to the colon [for which reason the term colon-specific antigen (CSA) has been retained], whereas the LMW/CSA was found in human gastrointestinal tissues, hamster and rat colon, human saliva, and normal human cervix. Colon-specific antigen (CSA) could be demonstrated in human gastrointestinal tumors, including the LS-174T colonic cancer cell line, but not in cancers of other sites tested. Likewise, CSA's were found in fetal human gut tissue. Whereas HMW/CSA and VHMW/CSA showed partial identity in immunodiffusion, HMW/CSA and VHMW/CSA, as well as LMW/CSA and VHMW/CSA, showed distinct immunoprecipitin bands, respectively. The immunoelectrophoretic mobility of VHMW/CSA was similar to an alpha-globulin, whereas HMW/CSA and LMW/CSA migrated to the prealbumin region. CSA appeared in immunofluorescence of GW-39 tumor cells and in the goblet cells of human colon predominantly as a cell-surface component. Staining with periodic acid-Schiff and solubility characteristics of the CSA's suggest that they are glycoprotein in nature. These studies thus support the view that organ-specific and organ-associated antigens of the colon can be maintained and expressed in human colonic carcinomas, including the xenografted GW-39 human colonic tumor system.", "PMID": 975104}
{"id": "pubmed23n0032_10174", "title": "Fetal antigens in human leukemia.", "content": "Immunization of BALB/c male mice with human peripheral leukemic blasts effectively reduced the later formation of syngeneic fetal liver, but not bone marrow hematopoietic colonies in the spleen when these mice were lethally irradiated and challenged i.v. Fetal antigen was detected in 6 of 6 lymphocytic leukemic patients and in 4 or 8 myelocytic leukemia patients and was correlated with low cellular levels of sialic acid. A rabbit antiserum ot BALB/c 15-day fetal liver cells labeled only 0 to 2% of normal donor peripheral leukocytes in indirect immunofluorescence but reacted with 10 to 21% of leukemic peripheral blasts. Active disease bone marrow on the same patients gave 7 to 40% fluorescent cells. Two remission bone marrow smaples were negative and 1 had 44% fluorescent cells. Using this antiserum coupled to sepharose, affinity column separation of KCl extracts from mouse and human fetal liver and from chronic lymphocytic leukemia has produced 4 common protein bands (identifiable on polyacrylamide gel electrophoresis).", "contents": "Fetal antigens in human leukemia. Immunization of BALB/c male mice with human peripheral leukemic blasts effectively reduced the later formation of syngeneic fetal liver, but not bone marrow hematopoietic colonies in the spleen when these mice were lethally irradiated and challenged i.v. Fetal antigen was detected in 6 of 6 lymphocytic leukemic patients and in 4 or 8 myelocytic leukemia patients and was correlated with low cellular levels of sialic acid. A rabbit antiserum ot BALB/c 15-day fetal liver cells labeled only 0 to 2% of normal donor peripheral leukocytes in indirect immunofluorescence but reacted with 10 to 21% of leukemic peripheral blasts. Active disease bone marrow on the same patients gave 7 to 40% fluorescent cells. Two remission bone marrow smaples were negative and 1 had 44% fluorescent cells. Using this antiserum coupled to sepharose, affinity column separation of KCl extracts from mouse and human fetal liver and from chronic lymphocytic leukemia has produced 4 common protein bands (identifiable on polyacrylamide gel electrophoresis).", "PMID": 975105}
{"id": "pubmed23n0032_10175", "title": "Human chorionic gonadotropin in human neoplastic cells.", "content": "Peroxidase-labeled antibody against the beta chain of human chorionic gonadotropin was used to demonstrate that 25 human malignant tumors contained this antigen. The antigen was localized both in the cytoplasm and on the surface of the malignant cells. Human chorionic gonadotropin may be responsible for both selective maternal immuno-suppression by fetal tissue and host immunosuppression by tumors.", "contents": "Human chorionic gonadotropin in human neoplastic cells. Peroxidase-labeled antibody against the beta chain of human chorionic gonadotropin was used to demonstrate that 25 human malignant tumors contained this antigen. The antigen was localized both in the cytoplasm and on the surface of the malignant cells. Human chorionic gonadotropin may be responsible for both selective maternal immuno-suppression by fetal tissue and host immunosuppression by tumors.", "PMID": 975106}
{"id": "pubmed23n0032_10176", "title": "Physical characterization and structural studies of the carcinoembryonic antigen.", "content": "Knowledge of the structure of carcinoembryonic antigen (CEA) is essential if we are to understand the relationship of this molecule to similar, possibly cross-reacting, molecules present in nonmalignant states. Electron microscopy shows that at neutral pH, CEA particles consist of homogeneous, morphologically distinctive, twisted rod-shaped particles, about 9 X 40 nm. The carbohydrate structure of CEA has been studied by periodate oxidation. All the N-acetylneuraminic acid and fucose and a portion of the galactose and mannose were destroyed by the first periodate treatment without altering immunological activity. N-Acetylneuraminic acid was shown to be linked to galactose since its prior removal with neuraminidase led to an equivalent increased destruction of galactose by one treatment with periodate. Significantly, even after 100% of the fucose and N-acetylneuraminic acid, 75% of the galactose, and 50% of the N-acetylglucosamine and mannose were destroyed by serial periodate oxidation (Smith degradation), the remaining portion of the CEA molecule lost no more antigenic activity than did control samples where periodate was omitted. No carbohydrate was lost or destroyed in the control reaction.", "contents": "Physical characterization and structural studies of the carcinoembryonic antigen. Knowledge of the structure of carcinoembryonic antigen (CEA) is essential if we are to understand the relationship of this molecule to similar, possibly cross-reacting, molecules present in nonmalignant states. Electron microscopy shows that at neutral pH, CEA particles consist of homogeneous, morphologically distinctive, twisted rod-shaped particles, about 9 X 40 nm. The carbohydrate structure of CEA has been studied by periodate oxidation. All the N-acetylneuraminic acid and fucose and a portion of the galactose and mannose were destroyed by the first periodate treatment without altering immunological activity. N-Acetylneuraminic acid was shown to be linked to galactose since its prior removal with neuraminidase led to an equivalent increased destruction of galactose by one treatment with periodate. Significantly, even after 100% of the fucose and N-acetylneuraminic acid, 75% of the galactose, and 50% of the N-acetylglucosamine and mannose were destroyed by serial periodate oxidation (Smith degradation), the remaining portion of the CEA molecule lost no more antigenic activity than did control samples where periodate was omitted. No carbohydrate was lost or destroyed in the control reaction.", "PMID": 975107}
{"id": "pubmed23n0032_10177", "title": "Immunochemical studies on carcinoembryonic antigen-reactive glycoproteins from carcinomas of the colon and breast separated by concanavalin A affinity chromatography.", "content": "Analysis of carcinoembryonic antigen (CE)-reactive glycoproteins from liver metastasis of primary colon and breast tumors and from primary breast tumors has been carried out by affinity chromatography on concanavalin A (Con A)-Sepharose. Three CEA-reactive glycoproteins from colon tumors (liver metastasis) with different binding capacity to Con A have been separated and further purified by gel filtration. Of the 3 CEA-reactive glycoproteins, 1 of them did not bind to Con A. Both Con A-binding and nonbinding CEA-reactive glycoproteins were immunologically indistinguishable when tested with a reference goat anti-CEA (ACE, 67-70; Dr. C.W. Todd and Dr. M.L. Egan), as well as with a variety of rabbit anti-CEA and anti-CEA (nonbinding) prepared in this laboratory. Carbohydrate analysis showed that mannose content of different purified CEA preparations or nonbinding CEA did not differ appreciably. N-Acetylglucosamine content of purified CEA preparations, however, varied considerably, suggesting that this sugar may impart the specificity of binding of CEA to Con A. The purified CEA preparations differed in their ability to inhibit the binding of 125l-labeled CEA to goat anti-CEA. One of the purified CEA preparations had 3- to 8-fold greater inhibitory capacity when compared to other preparations and shared a partial identity with a glycoprotein present in the extracts of fetal colon. The glycoprotein extracts of primary breast tumors did not contain a CEA that was immunologically identical to CEA present in colon tumors, whereas the liver metastasis of primary breast tumors showed several CEA-reactive glycoproteins as judged by radioimmunoassay. However, these CEA-reactive glycoproteins did not have any antigenic relationship with CEA from colon tumors when tested by double diffusion and immunoelectrophoresis. In conclusion, when Con A affinity chromatography of tumor glycoproteins is carried out under defined conditions and with the use of appropriate antisera, it is possible to delineate the presence or absence of CEA in tumors of nonentodermal origin.", "contents": "Immunochemical studies on carcinoembryonic antigen-reactive glycoproteins from carcinomas of the colon and breast separated by concanavalin A affinity chromatography. Analysis of carcinoembryonic antigen (CE)-reactive glycoproteins from liver metastasis of primary colon and breast tumors and from primary breast tumors has been carried out by affinity chromatography on concanavalin A (Con A)-Sepharose. Three CEA-reactive glycoproteins from colon tumors (liver metastasis) with different binding capacity to Con A have been separated and further purified by gel filtration. Of the 3 CEA-reactive glycoproteins, 1 of them did not bind to Con A. Both Con A-binding and nonbinding CEA-reactive glycoproteins were immunologically indistinguishable when tested with a reference goat anti-CEA (ACE, 67-70; Dr. C.W. Todd and Dr. M.L. Egan), as well as with a variety of rabbit anti-CEA and anti-CEA (nonbinding) prepared in this laboratory. Carbohydrate analysis showed that mannose content of different purified CEA preparations or nonbinding CEA did not differ appreciably. N-Acetylglucosamine content of purified CEA preparations, however, varied considerably, suggesting that this sugar may impart the specificity of binding of CEA to Con A. The purified CEA preparations differed in their ability to inhibit the binding of 125l-labeled CEA to goat anti-CEA. One of the purified CEA preparations had 3- to 8-fold greater inhibitory capacity when compared to other preparations and shared a partial identity with a glycoprotein present in the extracts of fetal colon. The glycoprotein extracts of primary breast tumors did not contain a CEA that was immunologically identical to CEA present in colon tumors, whereas the liver metastasis of primary breast tumors showed several CEA-reactive glycoproteins as judged by radioimmunoassay. However, these CEA-reactive glycoproteins did not have any antigenic relationship with CEA from colon tumors when tested by double diffusion and immunoelectrophoresis. In conclusion, when Con A affinity chromatography of tumor glycoproteins is carried out under defined conditions and with the use of appropriate antisera, it is possible to delineate the presence or absence of CEA in tumors of nonentodermal origin.", "PMID": 975108}
{"id": "pubmed23n0032_10178", "title": "Biochemical changes in premalignant intestines.", "content": "The immunological properties of thymidine kinase from a variety of human tumors suggest that the form of the tumor enzyme resembles that found in the placenta and in the nondividing colonic flat mucosa. To examine the placenta-like characteristics of tumor thymidine kinase, the jejunum and colon from rats ranging in age from fetal to old and from animals treated with dimethylhydrazine (DMH), an intestinal carcinogen, have been studied. In normal jejunum, thymidine kinase activity decreased rapidly with age. Both the activity and the response to phospholipase C and to mercaptans in DMH-induced tumors resembled that of fetal gut, while those in abnormal appearing DMH-treated jejunum were intermediate between normal control of the same age and tumor. Similar but less pronounced changes were seen in the colon. In the jejunum, the level of another enzyme normally associated with rapid cell division, ornithine decarboxylase, was found to be over 100 times that of the liver, colon, and stomach. Treatment of the animals with acetylaminofluorene and with DMH resulted in elevated levels of the enzyme in liver and in colon, respectively, but had little effect on this enzyme in other tissues. The data presented indicate that there were premalignant changes in the levels of both of these enzymes in target tissues of animals treated with carcinogens.", "contents": "Biochemical changes in premalignant intestines. The immunological properties of thymidine kinase from a variety of human tumors suggest that the form of the tumor enzyme resembles that found in the placenta and in the nondividing colonic flat mucosa. To examine the placenta-like characteristics of tumor thymidine kinase, the jejunum and colon from rats ranging in age from fetal to old and from animals treated with dimethylhydrazine (DMH), an intestinal carcinogen, have been studied. In normal jejunum, thymidine kinase activity decreased rapidly with age. Both the activity and the response to phospholipase C and to mercaptans in DMH-induced tumors resembled that of fetal gut, while those in abnormal appearing DMH-treated jejunum were intermediate between normal control of the same age and tumor. Similar but less pronounced changes were seen in the colon. In the jejunum, the level of another enzyme normally associated with rapid cell division, ornithine decarboxylase, was found to be over 100 times that of the liver, colon, and stomach. Treatment of the animals with acetylaminofluorene and with DMH resulted in elevated levels of the enzyme in liver and in colon, respectively, but had little effect on this enzyme in other tissues. The data presented indicate that there were premalignant changes in the levels of both of these enzymes in target tissues of animals treated with carcinogens.", "PMID": 975109}
{"id": "pubmed23n0032_10179", "title": "Expression of early fetal antigens on transformed mouse cells.", "content": "We have used immunoprecipitation of radioactivity from labeled cell membrane preparations to study further the association between the histocompatibility-2 (H-2) peptides and a tumor-associated embryonic antigen (Ag I) on the surface of L-cells. Since earlier studies had shown that Ag I and H-2 co-cap on L-cells, the present study was initiated to test the hypothesis that Ag I presents an altered H-2 peptide expressed on transformed cells. Indeed, sodium dodecyl sulfate-gel electrophoresis of Ag I shows two major peaks at approximately 110,000 and 40,000 daltons, the latter co-migrating with the major H-2 peptide. However, further analysis using sequential immunoprecipitation indicates that the molecules reacting with antisera to H-2 and to Ag I are separate entities, precipitating independently of one another. This has led us to postulate that Ag I and H-2 participate in a unique kind of association in the cell membrane, that is, that the bonds between them which allow them to co-cap may be in the lipid bilayer itself, and that these bonds are distrupted upon solubilization of the membrane.", "contents": "Expression of early fetal antigens on transformed mouse cells. We have used immunoprecipitation of radioactivity from labeled cell membrane preparations to study further the association between the histocompatibility-2 (H-2) peptides and a tumor-associated embryonic antigen (Ag I) on the surface of L-cells. Since earlier studies had shown that Ag I and H-2 co-cap on L-cells, the present study was initiated to test the hypothesis that Ag I presents an altered H-2 peptide expressed on transformed cells. Indeed, sodium dodecyl sulfate-gel electrophoresis of Ag I shows two major peaks at approximately 110,000 and 40,000 daltons, the latter co-migrating with the major H-2 peptide. However, further analysis using sequential immunoprecipitation indicates that the molecules reacting with antisera to H-2 and to Ag I are separate entities, precipitating independently of one another. This has led us to postulate that Ag I and H-2 participate in a unique kind of association in the cell membrane, that is, that the bonds between them which allow them to co-cap may be in the lipid bilayer itself, and that these bonds are distrupted upon solubilization of the membrane.", "PMID": 975110}
{"id": "pubmed23n0032_10180", "title": "The isolation and characterization of tumor-specific antigens of rodent and human tumors.", "content": "Putative tumor-specific transplantation antigens (TSTA) from both a carcinogen-induced rodent tumor (MC-1) and 2 human tumors were purified. The antigens were solubilized from the tumor cell membranes by limited papain digestion in a manner similar to that described for the isolation of normal histocompatibility antigens. The antitumor immune response of the tumor-bearing host was used to monitor the purification of the putative TSTA in both the rodent and human tumor systems. In the case of the rodent tumor, a major step in the purification of the TSTA involved affinity chromatography on Sepharose beads coupled to autologous antitumor antiserum. A comparable procedure was utilized in the purification of the TSTA from human tumors by using affinity chromatography on anti-human beta2-microglobulin antiserum coupled to a solid phase. The data obtained indicate that the TSTA of human tumors contains a beta2-microglobulin chain that is immunochemically identical with, and very similar in size to, that found in normal human histocompatibility antigens. A subunit of similar size was also identified in the carcinogen-induced rodent tumor. These results suggest that the TSTA in both humans and rodents may well be altered histocompatibility antigens.", "contents": "The isolation and characterization of tumor-specific antigens of rodent and human tumors. Putative tumor-specific transplantation antigens (TSTA) from both a carcinogen-induced rodent tumor (MC-1) and 2 human tumors were purified. The antigens were solubilized from the tumor cell membranes by limited papain digestion in a manner similar to that described for the isolation of normal histocompatibility antigens. The antitumor immune response of the tumor-bearing host was used to monitor the purification of the putative TSTA in both the rodent and human tumor systems. In the case of the rodent tumor, a major step in the purification of the TSTA involved affinity chromatography on Sepharose beads coupled to autologous antitumor antiserum. A comparable procedure was utilized in the purification of the TSTA from human tumors by using affinity chromatography on anti-human beta2-microglobulin antiserum coupled to a solid phase. The data obtained indicate that the TSTA of human tumors contains a beta2-microglobulin chain that is immunochemically identical with, and very similar in size to, that found in normal human histocompatibility antigens. A subunit of similar size was also identified in the carcinogen-induced rodent tumor. These results suggest that the TSTA in both humans and rodents may well be altered histocompatibility antigens.", "PMID": 975111}
{"id": "pubmed23n0032_10181", "title": "Cell surface alterations on colon adenocarcinoma cells.", "content": "By the use of five independent techniques, cell surface alterations distinctive of malignant as compared to normal colon cells were detected on in vivo surgical specimens and on cultured cell lines established in our laboratory. The findings, which were distinctive of the malignant as compared to the normal cell included: (a) polymorphism of surface microvilli on scan electron microscopy; (b) decreased susceptibility to infection with vaccinia and reovirus, but not to herpes, adeno- or echovirus: (c) production of large quantities of carcinoembryonic antigen; (d) presence of specific membrane proteins on sodium dodecyl sulfate-polyacrylamide gel analysis of plasma membranes purified from cell homogenates by ultracentrifugation in polyethylene glycol-dextran partitions; and (e) reaction with specific, cytotoxic, rabbit heteroantisera. Solubilized extracts of the malignant cells formed precipitin lines with the heteroantisera, suggesting that the distinctive antigens could be released from the cell surface. These results suggest that human colon carcinomas bear tumor-distinctive proteins and offer the prospect of specific immunodiagnostic reagents and immunotherapeutic tools.", "contents": "Cell surface alterations on colon adenocarcinoma cells. By the use of five independent techniques, cell surface alterations distinctive of malignant as compared to normal colon cells were detected on in vivo surgical specimens and on cultured cell lines established in our laboratory. The findings, which were distinctive of the malignant as compared to the normal cell included: (a) polymorphism of surface microvilli on scan electron microscopy; (b) decreased susceptibility to infection with vaccinia and reovirus, but not to herpes, adeno- or echovirus: (c) production of large quantities of carcinoembryonic antigen; (d) presence of specific membrane proteins on sodium dodecyl sulfate-polyacrylamide gel analysis of plasma membranes purified from cell homogenates by ultracentrifugation in polyethylene glycol-dextran partitions; and (e) reaction with specific, cytotoxic, rabbit heteroantisera. Solubilized extracts of the malignant cells formed precipitin lines with the heteroantisera, suggesting that the distinctive antigens could be released from the cell surface. These results suggest that human colon carcinomas bear tumor-distinctive proteins and offer the prospect of specific immunodiagnostic reagents and immunotherapeutic tools.", "PMID": 975112}
{"id": "pubmed23n0032_10182", "title": "Angiogenesis induction by tumors, embryonic tissues, and lymphocytes.", "content": "Although it is well established that tumors can induce endothelial cell mitosis and angiogenesis leading to extensive neovascularization, little is known concerning the spectrum of normal cells or tissues that may have similar inductive capabilities. A variety of developmentally active tissues were tested: mouse embryonic organ rudiments, mouse and rabbit trophoblast and placenta, and blastemal fragments obtained from regnerating deer antlers. Assays for angiogenesis included grafts made to the chorioallantoic membrane of chick embryos and grafts placed intracorneally in adult rabbit eyes. Virtually all of these test tissues were negative, the only  exceptions being some antler grafts and some grafts obtained from metanephric tubules of the embryonic mouse kidney.", "contents": "Angiogenesis induction by tumors, embryonic tissues, and lymphocytes. Although it is well established that tumors can induce endothelial cell mitosis and angiogenesis leading to extensive neovascularization, little is known concerning the spectrum of normal cells or tissues that may have similar inductive capabilities. A variety of developmentally active tissues were tested: mouse embryonic organ rudiments, mouse and rabbit trophoblast and placenta, and blastemal fragments obtained from regnerating deer antlers. Assays for angiogenesis included grafts made to the chorioallantoic membrane of chick embryos and grafts placed intracorneally in adult rabbit eyes. Virtually all of these test tissues were negative, the only  exceptions being some antler grafts and some grafts obtained from metanephric tubules of the embryonic mouse kidney.", "PMID": 975113}
{"id": "pubmed23n0032_10183", "title": "Cell cycle dependency of tumor antigens.", "content": "There is now substantial evidence that suggests that tumor antigen expression is cell cycle dependent. This information has been obtained from a wide variety of tumor systems. The G1 phase of the cell cycle has been implicated by a number of investigators as the point of maximal antigen appearance. In this presentation, we are concerned with a human sarcoma-associated tumor antigen and its cell cycle-dependent appearance. Sarcoma-associated tumor antigen is a membrane antigen present on cultured human neurosarcoma cells (T2-cells). Although the underlying mechanisms responsible for cell cycle-dependent expression are unclear, several possible interpretations are offered.", "contents": "Cell cycle dependency of tumor antigens. There is now substantial evidence that suggests that tumor antigen expression is cell cycle dependent. This information has been obtained from a wide variety of tumor systems. The G1 phase of the cell cycle has been implicated by a number of investigators as the point of maximal antigen appearance. In this presentation, we are concerned with a human sarcoma-associated tumor antigen and its cell cycle-dependent appearance. Sarcoma-associated tumor antigen is a membrane antigen present on cultured human neurosarcoma cells (T2-cells). Although the underlying mechanisms responsible for cell cycle-dependent expression are unclear, several possible interpretations are offered.", "PMID": 975114}
{"id": "pubmed23n0032_10184", "title": "[Condensation of D-glycosamines with proteins].", "content": "4-O-beta-D-Galactopyranosyl-alpha,beta-D-glucopyranosylamine (lactosylamine), beta-D-gluco-, alpha- and beta-D-galacto-, and beta-D-manno-pyranosylamines were bound to the carbodiimide-activated carboxyl groups of lysozyme. Of the 11 free carboxyl groups of the protein, approximately 3 were substituted by alpha,beta-lactosylamine, and approximately 2 by the monohexosylamines. One of the 4 glycopeptides isolated from the tryptic digest of the lysozyme-lactosylamine conjugate was identical to synthetic 1-N-L-leucinoyl-4-O-beta-D-galactopyranosyl-beta-D-glucopyranosylamine, indicating the substitution of the carboxyl group of the C-terminal leucine residue. The isolation of a glycopeptide containing the aspartic acid residue in position 117 indicates that the second alpha,beta-lactosylamine residue is linked to the carboxyl group of this amino acid. Both of the 2 other glycopeptides contain the same free carboxyl groups (one glutamic and two aspartic acid residues in positions 35, 48, and 52, respectively). The third alpha,beta-lactosylamine residue seems to be linked to one of these carboxyl groups.", "contents": "[Condensation of D-glycosamines with proteins]. 4-O-beta-D-Galactopyranosyl-alpha,beta-D-glucopyranosylamine (lactosylamine), beta-D-gluco-, alpha- and beta-D-galacto-, and beta-D-manno-pyranosylamines were bound to the carbodiimide-activated carboxyl groups of lysozyme. Of the 11 free carboxyl groups of the protein, approximately 3 were substituted by alpha,beta-lactosylamine, and approximately 2 by the monohexosylamines. One of the 4 glycopeptides isolated from the tryptic digest of the lysozyme-lactosylamine conjugate was identical to synthetic 1-N-L-leucinoyl-4-O-beta-D-galactopyranosyl-beta-D-glucopyranosylamine, indicating the substitution of the carboxyl group of the C-terminal leucine residue. The isolation of a glycopeptide containing the aspartic acid residue in position 117 indicates that the second alpha,beta-lactosylamine residue is linked to the carboxyl group of this amino acid. Both of the 2 other glycopeptides contain the same free carboxyl groups (one glutamic and two aspartic acid residues in positions 35, 48, and 52, respectively). The third alpha,beta-lactosylamine residue seems to be linked to one of these carboxyl groups.", "PMID": 975116}
{"id": "pubmed23n0032_10185", "title": "[Lengthening of the peptide chain at the C-terminal end of a glycosylamino acid].", "content": "An O-glycodipeptide was synthesized by lengthening the peptide chain on the C-terminal side of a glycosylamino acid unit. N-(Benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)-L-threonine o-nitrophenyl ester and pentachlorophenyl ester were condensed with glycine benzyl ester to give both the same glycodipeptide, [N-(benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)-L-threonyl]glycine benzyl ester (9). The two anomers, N-(benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-alpha- and beta-D-glucopyranosyl)-L-threonine pentachlorophenyl ester were also prepared. 1H-N.m.r. studies ascertained the structure and anomeric configuration expected.", "contents": "[Lengthening of the peptide chain at the C-terminal end of a glycosylamino acid]. An O-glycodipeptide was synthesized by lengthening the peptide chain on the C-terminal side of a glycosylamino acid unit. N-(Benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)-L-threonine o-nitrophenyl ester and pentachlorophenyl ester were condensed with glycine benzyl ester to give both the same glycodipeptide, [N-(benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)-L-threonyl]glycine benzyl ester (9). The two anomers, N-(benzyloxycarbonyl)-3-O-(2,3,4,6-tetra-O-acetyl-alpha- and beta-D-glucopyranosyl)-L-threonine pentachlorophenyl ester were also prepared. 1H-N.m.r. studies ascertained the structure and anomeric configuration expected.", "PMID": 975118}
{"id": "pubmed23n0032_10186", "title": "Characterization of the fragments obtained by enzymic and alkaline degradation of rice-bran proteoglycans.", "content": "Repeated digestion of the proteoglycan B from rice bran with Pronase and hemicellulase yielded two types of glycopeptides. The first group, obtained from the third enzymic digest, was a type of low molecular-weight glycopeptide and was fractionated into two subfractions by paper electrophoresis. The second group, obtained from the fourth enzymic digest, was a mixture of glycopeptides of relatively high molecular-weight and was fractionated into three subfractions by gel filtration on Sephadex G-75. Alkaline degradation of these glycopeptides or the intact proteoglycan A yielded a sugar-amino acid compound, whose structure was established as O-alpha-L-arabinofuranosyl-hydroxyproline. These results indicate that the carbohydrate-protein linkage of the rice-bran proteoglycan is an O-glycosyl linkage between L-arabinose and hydroxyproline.", "contents": "Characterization of the fragments obtained by enzymic and alkaline degradation of rice-bran proteoglycans. Repeated digestion of the proteoglycan B from rice bran with Pronase and hemicellulase yielded two types of glycopeptides. The first group, obtained from the third enzymic digest, was a type of low molecular-weight glycopeptide and was fractionated into two subfractions by paper electrophoresis. The second group, obtained from the fourth enzymic digest, was a mixture of glycopeptides of relatively high molecular-weight and was fractionated into three subfractions by gel filtration on Sephadex G-75. Alkaline degradation of these glycopeptides or the intact proteoglycan A yielded a sugar-amino acid compound, whose structure was established as O-alpha-L-arabinofuranosyl-hydroxyproline. These results indicate that the carbohydrate-protein linkage of the rice-bran proteoglycan is an O-glycosyl linkage between L-arabinose and hydroxyproline.", "PMID": 975120}
{"id": "pubmed23n0032_10187", "title": "The limit dextrinase from malted sorghum (Sorghum vulgare).", "content": "A limit dextrinase, free from contaminating carbohydrases, has been purified from malted sorghum flour. The enzyme readily hydrolysed alpha-limit dextrins having maltosyl or maltotriosyl side-chains, pullulan, and amylopectin beta-limit dextrin. Glycogen beta-limit dextrin and amylopectin were more slowly hydrolysed, the detection of the hydrolysis of amylopectin being dependent on enzyme concentration. No significant debranching of glycogen could be detected.", "contents": "The limit dextrinase from malted sorghum (Sorghum vulgare). A limit dextrinase, free from contaminating carbohydrases, has been purified from malted sorghum flour. The enzyme readily hydrolysed alpha-limit dextrins having maltosyl or maltotriosyl side-chains, pullulan, and amylopectin beta-limit dextrin. Glycogen beta-limit dextrin and amylopectin were more slowly hydrolysed, the detection of the hydrolysis of amylopectin being dependent on enzyme concentration. No significant debranching of glycogen could be detected.", "PMID": 975121}
{"id": "pubmed23n0032_10188", "title": "Autonomic neural control of cardiac rhythm: the role of autonomic imbalance in the genesis of cardiac dysrhythmia.", "content": "Cardiac dysrhythmias result from abnormalities in rate, regularity, or sequence of cardiac activation, and because of direct actions of the autonomic nervous system upon each of these properties, imbalance in this system may play an important role in the genesis of cardiac dysrhythmia. A canine model has been developed in which the extrinsic innervation of the heart is ablated with the exception of the ventrolateral cardiac nerve. This nerve is distributed primarily to the inferior atrial, AV junctional, and ventricular tissues. Following recovery from surgery, the animal is placed on a treadmill and required to perform strenuous exercise. In all of six animals which sustained repeated exercise testing over periods of 4-12 months, dysrhythmias of varying complexities were elicited. None appeared in parallel experiments conducted in control or sham-operated animals. The dysrhythmias consisted of supraventricular, AV junctional, or ventricular tachycardias with occasional premature atrial or ventricular systoles. The dysrhythmias were not influenced by atropine but were generally controlled by propranolol.", "contents": "Autonomic neural control of cardiac rhythm: the role of autonomic imbalance in the genesis of cardiac dysrhythmia. Cardiac dysrhythmias result from abnormalities in rate, regularity, or sequence of cardiac activation, and because of direct actions of the autonomic nervous system upon each of these properties, imbalance in this system may play an important role in the genesis of cardiac dysrhythmia. A canine model has been developed in which the extrinsic innervation of the heart is ablated with the exception of the ventrolateral cardiac nerve. This nerve is distributed primarily to the inferior atrial, AV junctional, and ventricular tissues. Following recovery from surgery, the animal is placed on a treadmill and required to perform strenuous exercise. In all of six animals which sustained repeated exercise testing over periods of 4-12 months, dysrhythmias of varying complexities were elicited. None appeared in parallel experiments conducted in control or sham-operated animals. The dysrhythmias consisted of supraventricular, AV junctional, or ventricular tachycardias with occasional premature atrial or ventricular systoles. The dysrhythmias were not influenced by atropine but were generally controlled by propranolol.", "PMID": 975122}
{"id": "pubmed23n0032_10189", "title": "Role of the nervous system in experimentally induced arrhythmias.", "content": "The purpose of our studies was to examine the role of the nervous system in arrhythmias produced by digitalis overdose and coronary artery occlusion in the cat. This was done by observing the effect of these arrhythmogenic procedures on cardiac efferent neural activity and then determining whether any observed alteration in neural activity contributed to the cardiac rhythm disturbances evoked by digitalis and coronary artery occlusion. Our data indicate that both procedures used to evoke arrhythmias activate each division of the autonomic nervous system. Activation of the sympathetic nervous system resulted in a deleterious effect on cardiac rhythm whereas activation of the parasympathetic nervous system, in general, resulted in a beneficial effect on cardiac rhythm. With coronary occlusion, the role exerted by the nervous system depended on the anatomic location of the involved myocardium. Studies directed at elucidating the mechanisms whereby the nervous system caused cardiac rhythm disturbances indicated that there may be an important difference between the antiarrhythmic efficacy of beta-adrenergic blockade and bilateral stellate ganglionectomy. The latter procedure proved to be a more effective way of removing deleterious sympathetic neural effects on the heart. In conclusion, our findings suggest that the development of new drugs for treating arrhythmias resulting from digitalis and coronary occlusion should be aimed at finding drugs that act to either depress central sympathetic outflow or enhance parasympathetic effects on the ventricle.", "contents": "Role of the nervous system in experimentally induced arrhythmias. The purpose of our studies was to examine the role of the nervous system in arrhythmias produced by digitalis overdose and coronary artery occlusion in the cat. This was done by observing the effect of these arrhythmogenic procedures on cardiac efferent neural activity and then determining whether any observed alteration in neural activity contributed to the cardiac rhythm disturbances evoked by digitalis and coronary artery occlusion. Our data indicate that both procedures used to evoke arrhythmias activate each division of the autonomic nervous system. Activation of the sympathetic nervous system resulted in a deleterious effect on cardiac rhythm whereas activation of the parasympathetic nervous system, in general, resulted in a beneficial effect on cardiac rhythm. With coronary occlusion, the role exerted by the nervous system depended on the anatomic location of the involved myocardium. Studies directed at elucidating the mechanisms whereby the nervous system caused cardiac rhythm disturbances indicated that there may be an important difference between the antiarrhythmic efficacy of beta-adrenergic blockade and bilateral stellate ganglionectomy. The latter procedure proved to be a more effective way of removing deleterious sympathetic neural effects on the heart. In conclusion, our findings suggest that the development of new drugs for treating arrhythmias resulting from digitalis and coronary occlusion should be aimed at finding drugs that act to either depress central sympathetic outflow or enhance parasympathetic effects on the ventricle.", "PMID": 975123}
{"id": "pubmed23n0032_10190", "title": "Neural basis for the genesis and control of digitalis arrhythmias.", "content": "The pharmacokinetics of ouabain associated with toxicity were studied in the cat and the guinea pig both in vivo and in vitro using ouabain-H3. After spinal cord transection a higher dose of ouabain was required to reach the lethal endpoint. This intervention also increased the myocardial and serum levels associated with toxicity were studied in the cat and the guinea pig both in vivo and in vitro using ouabain-H3. After spinal cord transection a higher dose of ouabain was required to reach the lethal endpoint. This intervention also increased the myocardial and serum levels associated with death. These findings were corroborated in experiments using digitoxin H3. In vitro, substantially higher ouabain tissue contents were associated with a lethal event. In addition, in cats and guinea pigs, the lethal myocardial ouabain content did not change when the infusion rate of ouabain was varied in vivo or the perfusate ouabain concentration was changed in vitro. In vivo, propranolol increases the myocardial ouabain content associated with death to in vitro levels. In vitro, the drugs prolongs the time to death by retarding the myocardial uptake of ouabain. These data suggest that the toxic effects of ouabain in the whole animal are largely neural and in the isolated heart, substantially myocardial.", "contents": "Neural basis for the genesis and control of digitalis arrhythmias. The pharmacokinetics of ouabain associated with toxicity were studied in the cat and the guinea pig both in vivo and in vitro using ouabain-H3. After spinal cord transection a higher dose of ouabain was required to reach the lethal endpoint. This intervention also increased the myocardial and serum levels associated with toxicity were studied in the cat and the guinea pig both in vivo and in vitro using ouabain-H3. After spinal cord transection a higher dose of ouabain was required to reach the lethal endpoint. This intervention also increased the myocardial and serum levels associated with death. These findings were corroborated in experiments using digitoxin H3. In vitro, substantially higher ouabain tissue contents were associated with a lethal event. In addition, in cats and guinea pigs, the lethal myocardial ouabain content did not change when the infusion rate of ouabain was varied in vivo or the perfusate ouabain concentration was changed in vitro. In vivo, propranolol increases the myocardial ouabain content associated with death to in vitro levels. In vitro, the drugs prolongs the time to death by retarding the myocardial uptake of ouabain. These data suggest that the toxic effects of ouabain in the whole animal are largely neural and in the isolated heart, substantially myocardial.", "PMID": 975124}
{"id": "pubmed23n0032_10191", "title": "Central nervous system control of cardiac rhythm.", "content": "Stimulation of sites in the midbrain reticular formation and in the posterior hypothalamus of the cat resulted in a large to modest rise of arterial pressure and the induction of cardiac dysrhythmias. Most frequently, these arrhythmias developed upon cessation of brain stem stimulation but also occurred during the stimulus period in 5 of 23 cats studied. The arrhythmias disappeared upon cooling and reappeared upon rewarming the vagus nerves. The ventricular dysrhythmias also were abolished by methylscopolamine, by bilateral vagatomy, or by extirpation of the stellate ganglia. Simultaneous stimulation of both distal end of the cut right vagus nerve and the decentralized right stellate ganglion caused arrhythmias similar to those observed after diencephalic stimulation. These data are interpreted to indicate that the cardiac arrhythmias evoked by brain stem stimulation result from the interplay of both sympathetic and parasympathetic influences on the heart. The response patterns of a population of medullary neurons activated by carotid sinus nerve stimulation were modified by condition stimuli to posterior hypothalamic sites. From studies on unit activity of brain stem areas known to participate in cardiovascular adjustment, a schema is proposed of hypothalamic-medulla interaction as a central mechanism that may account for the development of ventricular arrhythmias.", "contents": "Central nervous system control of cardiac rhythm. Stimulation of sites in the midbrain reticular formation and in the posterior hypothalamus of the cat resulted in a large to modest rise of arterial pressure and the induction of cardiac dysrhythmias. Most frequently, these arrhythmias developed upon cessation of brain stem stimulation but also occurred during the stimulus period in 5 of 23 cats studied. The arrhythmias disappeared upon cooling and reappeared upon rewarming the vagus nerves. The ventricular dysrhythmias also were abolished by methylscopolamine, by bilateral vagatomy, or by extirpation of the stellate ganglia. Simultaneous stimulation of both distal end of the cut right vagus nerve and the decentralized right stellate ganglion caused arrhythmias similar to those observed after diencephalic stimulation. These data are interpreted to indicate that the cardiac arrhythmias evoked by brain stem stimulation result from the interplay of both sympathetic and parasympathetic influences on the heart. The response patterns of a population of medullary neurons activated by carotid sinus nerve stimulation were modified by condition stimuli to posterior hypothalamic sites. From studies on unit activity of brain stem areas known to participate in cardiovascular adjustment, a schema is proposed of hypothalamic-medulla interaction as a central mechanism that may account for the development of ventricular arrhythmias.", "PMID": 975125}
{"id": "pubmed23n0032_10192", "title": "Persistence of a basal lamina-like structure following DOCA-induced myofibrillar degeneration in rats.", "content": "It is known that the myocardial necrosis of potassium depletion heals by reconstitution and not by scarring, when animals are replenished with potassium. In the course of studies on DOCA-induced myofibrillar degeneration, we found that a membrane-like structure persisted in the area where the disintegrating myofiber was being removed by macrophages. This structure resembled the basal lamina, and enclosed a space in which macrophages with phagosomes containing disintegrating myofibral constituents were seen in association with undifferentiated cells. We postulate that this basal lamina-like structure along with the undifferentiated cells, play a role in the reconstitution of the myocardium during the stage of potassium repletion, and that the scaffolding by basal lamina may be effective in myocardial reconstitution.", "contents": "Persistence of a basal lamina-like structure following DOCA-induced myofibrillar degeneration in rats. It is known that the myocardial necrosis of potassium depletion heals by reconstitution and not by scarring, when animals are replenished with potassium. In the course of studies on DOCA-induced myofibrillar degeneration, we found that a membrane-like structure persisted in the area where the disintegrating myofiber was being removed by macrophages. This structure resembled the basal lamina, and enclosed a space in which macrophages with phagosomes containing disintegrating myofibral constituents were seen in association with undifferentiated cells. We postulate that this basal lamina-like structure along with the undifferentiated cells, play a role in the reconstitution of the myocardium during the stage of potassium repletion, and that the scaffolding by basal lamina may be effective in myocardial reconstitution.", "PMID": 975126}
{"id": "pubmed23n0032_10193", "title": "Familial cardiomyopathy.", "content": "Over three generations, several members of a family suffered from cardiomyopathy exhibiting, primarily, rhythm and conduction disturbances and, eventually, congestive heart failure. Certain serum enzymes (SGOT, SGPT, LDH) were elevated. The clinical examination indicated a progressive deterioration with increasing age of the patients, a more serious course in the male members of the family, and the frequent association of mental retardation.", "contents": "Familial cardiomyopathy. Over three generations, several members of a family suffered from cardiomyopathy exhibiting, primarily, rhythm and conduction disturbances and, eventually, congestive heart failure. Certain serum enzymes (SGOT, SGPT, LDH) were elevated. The clinical examination indicated a progressive deterioration with increasing age of the patients, a more serious course in the male members of the family, and the frequent association of mental retardation.", "PMID": 975127}
{"id": "pubmed23n0032_10194", "title": "Changes in muscle blood flow and development of the arm following the Blalock-Taussig anastomosis.", "content": "Muscle blood flow was studied in 28 patients after Blalock-Taussig anastomosis using the radioactive xenon clearance method. Insignificant differences in blood flow between the arms were found at rest, but there was a highly significant lowering of the peak blood flow on the operated side. Peak blood flow values in the first years after the anastomosis were close to the values in obliterative artery disease. The difference in maximum blood flow became less apparent beyond the first 5 years after the operation. A prolonged time of onset and duration of hyperemia after ischemic exercise were observed on the side of the operation. Statistically significant differences in circumference and length between the arms were measured and a lower strength of contraction of the affected hand was demonstrated. Abnormally low blood supply to muscles is thought to be responsible for the anatomical changes in the operated extremity.", "contents": "Changes in muscle blood flow and development of the arm following the Blalock-Taussig anastomosis. Muscle blood flow was studied in 28 patients after Blalock-Taussig anastomosis using the radioactive xenon clearance method. Insignificant differences in blood flow between the arms were found at rest, but there was a highly significant lowering of the peak blood flow on the operated side. Peak blood flow values in the first years after the anastomosis were close to the values in obliterative artery disease. The difference in maximum blood flow became less apparent beyond the first 5 years after the operation. A prolonged time of onset and duration of hyperemia after ischemic exercise were observed on the side of the operation. Statistically significant differences in circumference and length between the arms were measured and a lower strength of contraction of the affected hand was demonstrated. Abnormally low blood supply to muscles is thought to be responsible for the anatomical changes in the operated extremity.", "PMID": 975128}
{"id": "pubmed23n0032_10195", "title": "Operant conditioning of heart rate in free-moving dogs.", "content": "The magnitude of changes in heart rate that could be induced by the operant conditioning of beat-to-beat intervals was determined. Shock avoidance methods were employed during 71 sessions conducted over 17 weeks in 3 free-moving mongrel dogs. Experimental periods during which conditioning was done alternated with baseline periods. Heart rate differences between baseline and experimental periods were used as indices of conditioning. Alterations in  heart rate were compared among four types of sessions: control, random shock, decrement conditioning and increment conditioning. Conditioned heart rate decrements averaged 3% while heart rate increments averaged 10%. Thus, operant conditioning carried out over prolonged periods of time resulted in relatively minor changes in heart rate.", "contents": "Operant conditioning of heart rate in free-moving dogs. The magnitude of changes in heart rate that could be induced by the operant conditioning of beat-to-beat intervals was determined. Shock avoidance methods were employed during 71 sessions conducted over 17 weeks in 3 free-moving mongrel dogs. Experimental periods during which conditioning was done alternated with baseline periods. Heart rate differences between baseline and experimental periods were used as indices of conditioning. Alterations in  heart rate were compared among four types of sessions: control, random shock, decrement conditioning and increment conditioning. Conditioned heart rate decrements averaged 3% while heart rate increments averaged 10%. Thus, operant conditioning carried out over prolonged periods of time resulted in relatively minor changes in heart rate.", "PMID": 975129}
{"id": "pubmed23n0032_10196", "title": "Manifestation of the Wolff-Parkinson-White syndrome during isoprenaline infusion and carotid sinus massage.", "content": "In a patient with transient WPW syndrome the electrocardiographic pattern of preexcitation could repeatedly be induced by carotid sinus massage during isoprenaline infusion. Adrenergic and cholinergic discharge may favor the conduction of the impulse over an accessory nonfunctional A-V bundle.", "contents": "Manifestation of the Wolff-Parkinson-White syndrome during isoprenaline infusion and carotid sinus massage. In a patient with transient WPW syndrome the electrocardiographic pattern of preexcitation could repeatedly be induced by carotid sinus massage during isoprenaline infusion. Adrenergic and cholinergic discharge may favor the conduction of the impulse over an accessory nonfunctional A-V bundle.", "PMID": 975130}
{"id": "pubmed23n0032_10197", "title": "Multifascicular block in cardiomyopathy. Incidence, localization by His bundle study and prognosis.", "content": "Of 70 patients with cardiomyopathy, nine (13%) had bifascicular block: right bundle branch block with left anterior hemiblock in four, right bundle branch block with left posterior hemiblock in two, and left bundle branch block with left anterior hemiblock in three. Isolated left bundle branch block was present in four, isolated left anterior hemiblock in 13, and isolated left posterior hemiblock in one. No isolated right bundle branch block was found. Five of the patients with bifascicular block were evaluated by His bundle electrocardiography and atrial pacing. Block in the His-Purkinje system (prolonged HV interval) was present in four and atrioventricular conduction (AH interval) was normal in all five. Despite a common prolongation in intraventricular conduction, short term prognosis seems to be favorable since: (1) none had spontaneous progression to more advanced block or experienced Stokes-Adams attacks for an average follow-up period of at least 42 months, and (2) with atrial pacing, one-to-one atrioventricular conduction persisted to rates up to 150/min in all patients with no further prolongation of HV interval.", "contents": "Multifascicular block in cardiomyopathy. Incidence, localization by His bundle study and prognosis. Of 70 patients with cardiomyopathy, nine (13%) had bifascicular block: right bundle branch block with left anterior hemiblock in four, right bundle branch block with left posterior hemiblock in two, and left bundle branch block with left anterior hemiblock in three. Isolated left bundle branch block was present in four, isolated left anterior hemiblock in 13, and isolated left posterior hemiblock in one. No isolated right bundle branch block was found. Five of the patients with bifascicular block were evaluated by His bundle electrocardiography and atrial pacing. Block in the His-Purkinje system (prolonged HV interval) was present in four and atrioventricular conduction (AH interval) was normal in all five. Despite a common prolongation in intraventricular conduction, short term prognosis seems to be favorable since: (1) none had spontaneous progression to more advanced block or experienced Stokes-Adams attacks for an average follow-up period of at least 42 months, and (2) with atrial pacing, one-to-one atrioventricular conduction persisted to rates up to 150/min in all patients with no further prolongation of HV interval.", "PMID": 975131}
{"id": "pubmed23n0032_10198", "title": "Clinical utility of the simultaneous recording of heart and Korotkoff sounds.", "content": "Heart and arterial sounds and murmurs for a sequence of as many as 100 successive beats are recorded on a single print. The apparatus consists of a cathode ray oscilloscope, a Q-wave trigger to release the beam, a vertical axis which is connected to the blood pressure cuff, and an intensity modulation circuit which determines the intensity of the beam at each instant. This arrangement eliminates the baseline and thereby permits the display of low intensity acoustic signals. Data on normal subjects, and patients with various heart diseases are illustrated. Advantages and limitations of the method are discussed.", "contents": "Clinical utility of the simultaneous recording of heart and Korotkoff sounds. Heart and arterial sounds and murmurs for a sequence of as many as 100 successive beats are recorded on a single print. The apparatus consists of a cathode ray oscilloscope, a Q-wave trigger to release the beam, a vertical axis which is connected to the blood pressure cuff, and an intensity modulation circuit which determines the intensity of the beam at each instant. This arrangement eliminates the baseline and thereby permits the display of low intensity acoustic signals. Data on normal subjects, and patients with various heart diseases are illustrated. Advantages and limitations of the method are discussed.", "PMID": 975132}
{"id": "pubmed23n0032_10199", "title": "[Relation between training status and orthostatic tolerance].", "content": "Investigations on orthostatic tolerance and physical fitness prior to and after 6-8 h immersion experiments surprisingly revealed that all untrained subjects endured pre- and postimmersion tilt table tests without subjective complaints, whereas after immersion all trained participants collapsed within the first minutes of erect position displaying symptoms of a vasovagal syncope. Assuming that this impaired orthostatic tolerance of trained subjects can partially be referred to influences of an altered blood pressure control system we decided to record the blood pressure characteristics of both groups. Thereby, the transmural pressure of the carotid artery was changed by applying from outside either reduced or exceeding pressures. The controller sensitivity in the untrained group was almost twice as high as in the trained one. This implies a better regulatory response to disturbing interference in the untrained. Under normal conditions the controlling capacity of both groups is sufficient to cope with body position changes. However, during stress situations--in this case, reduction of aldosterone concentration as well as losses of plasma volume due to the 'Gauer-Henry-Reflex'--blood pressure regulation first fails in the trained subjects.", "contents": "[Relation between training status and orthostatic tolerance]. Investigations on orthostatic tolerance and physical fitness prior to and after 6-8 h immersion experiments surprisingly revealed that all untrained subjects endured pre- and postimmersion tilt table tests without subjective complaints, whereas after immersion all trained participants collapsed within the first minutes of erect position displaying symptoms of a vasovagal syncope. Assuming that this impaired orthostatic tolerance of trained subjects can partially be referred to influences of an altered blood pressure control system we decided to record the blood pressure characteristics of both groups. Thereby, the transmural pressure of the carotid artery was changed by applying from outside either reduced or exceeding pressures. The controller sensitivity in the untrained group was almost twice as high as in the trained one. This implies a better regulatory response to disturbing interference in the untrained. Under normal conditions the controlling capacity of both groups is sufficient to cope with body position changes. However, during stress situations--in this case, reduction of aldosterone concentration as well as losses of plasma volume due to the 'Gauer-Henry-Reflex'--blood pressure regulation first fails in the trained subjects.", "PMID": 975140}
{"id": "pubmed23n0032_10200", "title": "Use of an elastic garment in the treatment of orthostatic hypotension.", "content": "The most common cause of orthostatic hypotension is poor postural adjustment. The most severe form of orthostatic hypotension is of unknown etiology; the primary disorder is a degeneration of the peripheral autonomic nervous system, resulting in a decrease in production of catecholamine. Physiologically, there is a failure of arteriolar and venous vasoconstriction and a lack of cardiac response, both in rate and in contractility to sympathetic stimulation. Total and central cardiopulmonary blood volumes are contracted. The application of hydrostatic counterpressure by the use of an elasticized garment is helpful in the management of orthostatic hypotension, especially when used with sodium-retaining steroids to increase intravascular volume.", "contents": "Use of an elastic garment in the treatment of orthostatic hypotension. The most common cause of orthostatic hypotension is poor postural adjustment. The most severe form of orthostatic hypotension is of unknown etiology; the primary disorder is a degeneration of the peripheral autonomic nervous system, resulting in a decrease in production of catecholamine. Physiologically, there is a failure of arteriolar and venous vasoconstriction and a lack of cardiac response, both in rate and in contractility to sympathetic stimulation. Total and central cardiopulmonary blood volumes are contracted. The application of hydrostatic counterpressure by the use of an elasticized garment is helpful in the management of orthostatic hypotension, especially when used with sodium-retaining steroids to increase intravascular volume.", "PMID": 975141}
{"id": "pubmed23n0032_10201", "title": "Pharmacological basis of the treatment of orthostatic disorders with ergot alkaloids.", "content": "Ergot alkaloids increase the tone of isolated canine vein strips dose-dependently in concentrations considerably lower than noradrenaline, but the maximal responses are only about one third of those to noradrenaline. This action can be blocked by prior administration of phentolamine. It is therefore concluded that the long-lasting stimulant action of ergot alkaloids on vascular smooth muscle is mediated mainly by alpha-adrenoceptors. Using the autoperfused hind limb of the cat, it has been shown that dihydroergotamine increases dose-dependently the tone of the capacitance vessels in a manner very similar to electrical stimulation of the sympathetic nerve with increasing frequencies. However, in contrast to sympathetic nerve stimulation, dihydroergotamine elicits only a very weak increase in arteriolar resistance.", "contents": "Pharmacological basis of the treatment of orthostatic disorders with ergot alkaloids. Ergot alkaloids increase the tone of isolated canine vein strips dose-dependently in concentrations considerably lower than noradrenaline, but the maximal responses are only about one third of those to noradrenaline. This action can be blocked by prior administration of phentolamine. It is therefore concluded that the long-lasting stimulant action of ergot alkaloids on vascular smooth muscle is mediated mainly by alpha-adrenoceptors. Using the autoperfused hind limb of the cat, it has been shown that dihydroergotamine increases dose-dependently the tone of the capacitance vessels in a manner very similar to electrical stimulation of the sympathetic nerve with increasing frequencies. However, in contrast to sympathetic nerve stimulation, dihydroergotamine elicits only a very weak increase in arteriolar resistance.", "PMID": 975143}
{"id": "pubmed23n0032_10202", "title": "[Effect of ergot alkaloids on venous tonus determined in superficial hand veins in situ].", "content": "The venoconstrictor effect of dihydroergotamine in man was demonstrated by Rieckert and Pauschinger in 1967. In a study on the influence of local infusions of dihydroergotamine into superficial hand veins in man we found a direct venoconstrictor effect of the drug. In further studies the effects of dihydroergotamine were compared with those of other dihydrogenated and non-dihydrogenated ergot alkaloids and a dose-dependent venoconstrictor effect was observed after local infusion of dihydroergotamine, dihydroergostine, dihydroergovaline, ergotamine and methysergide into superficial hand veins in man. Dihydroergotamine was weight for weight somewhat less active than ergotamine. But as Mellander and Nordenfelt have shown that dihydroergotamine elicits only a weak effect on resistance vessels, this drug seems for the treatment of orthostatic hypotension to be preferable to ergotamine for which Owen and St\u00fcrmer have demonstrated a marked constriction of resistance vessels in the skin. Experiment with dihydroergotamine showed that the venoconstriction in these human veins is mainly due to alpha-adrenoceptor stimulation. As in the treatment of orthostatic hypotension drugs are usually given in oral form, the activity of dihydroergotamine was also investigated after oral administration and the venoconstrictor activity confirmed.", "contents": "[Effect of ergot alkaloids on venous tonus determined in superficial hand veins in situ]. The venoconstrictor effect of dihydroergotamine in man was demonstrated by Rieckert and Pauschinger in 1967. In a study on the influence of local infusions of dihydroergotamine into superficial hand veins in man we found a direct venoconstrictor effect of the drug. In further studies the effects of dihydroergotamine were compared with those of other dihydrogenated and non-dihydrogenated ergot alkaloids and a dose-dependent venoconstrictor effect was observed after local infusion of dihydroergotamine, dihydroergostine, dihydroergovaline, ergotamine and methysergide into superficial hand veins in man. Dihydroergotamine was weight for weight somewhat less active than ergotamine. But as Mellander and Nordenfelt have shown that dihydroergotamine elicits only a weak effect on resistance vessels, this drug seems for the treatment of orthostatic hypotension to be preferable to ergotamine for which Owen and St\u00fcrmer have demonstrated a marked constriction of resistance vessels in the skin. Experiment with dihydroergotamine showed that the venoconstriction in these human veins is mainly due to alpha-adrenoceptor stimulation. As in the treatment of orthostatic hypotension drugs are usually given in oral form, the activity of dihydroergotamine was also investigated after oral administration and the venoconstrictor activity confirmed.", "PMID": 975144}
{"id": "pubmed23n0032_10203", "title": "Circulatory effects of dihydroergotamine in patients with disturbed sympathetic vasomotor control with special reference to postural hypotension.", "content": "In five patients with postural hypotension (disturbed sympathetic vasomotor control) the effect of intravenous and oral administration of dihydroergotamin (DHE) has been studied. The therapeutic effect of oral administration of DHE has also been compared to the effect of treatment with an antigravity suit. Following intravenous administration of DHE, systemic and central venous pressure increased in all cases. The effect on cardiac output was negligible and consequently the calculated total peripheral vascular resistance increased. Orthostatic tolerance increased in all cases. During long-term oral administration the effect was comparable to that obtained with an antigravity suit. The increased orthostatic tolerance is explained by a constrictive effect on both capacitance and resistance vessels. In order to analyze the influence of a functional denervation on the effect of DHE on peripheral circulation, a study has also been performed during epidural anaesthesia of the lower body in 11 patients. In the legs which were deprived of sympathetic tone intravenous administration of DHE caused constriction of resistance vessels. In the intact forearm there was a decrease of tone in the resistance vessels. The results indicate that DHE - apart from its well-known venoconstrictive effect - also may constrict resistance vessels when they are subjected to a low sympathetic nervous outflow.", "contents": "Circulatory effects of dihydroergotamine in patients with disturbed sympathetic vasomotor control with special reference to postural hypotension. In five patients with postural hypotension (disturbed sympathetic vasomotor control) the effect of intravenous and oral administration of dihydroergotamin (DHE) has been studied. The therapeutic effect of oral administration of DHE has also been compared to the effect of treatment with an antigravity suit. Following intravenous administration of DHE, systemic and central venous pressure increased in all cases. The effect on cardiac output was negligible and consequently the calculated total peripheral vascular resistance increased. Orthostatic tolerance increased in all cases. During long-term oral administration the effect was comparable to that obtained with an antigravity suit. The increased orthostatic tolerance is explained by a constrictive effect on both capacitance and resistance vessels. In order to analyze the influence of a functional denervation on the effect of DHE on peripheral circulation, a study has also been performed during epidural anaesthesia of the lower body in 11 patients. In the legs which were deprived of sympathetic tone intravenous administration of DHE caused constriction of resistance vessels. In the intact forearm there was a decrease of tone in the resistance vessels. The results indicate that DHE - apart from its well-known venoconstrictive effect - also may constrict resistance vessels when they are subjected to a low sympathetic nervous outflow.", "PMID": 975147}
{"id": "pubmed23n0032_10204", "title": "Neurogenic influences on blood pressure and vascular tone from peripheral receptors during muscular contraction.", "content": "In anesthetized dogs, contraction of the muscles of the thigh was elicited either by applying electrodes in the muscles or by stimulation of the spinal ventral roots. The carotid sinuses were isolated; the vagi and the depressor nerves cut. Rhythmic muscular contraction caused hypotension and vasodilatation; tetanic contraction caused hypertension and vasoconstriction. These responses are of reflexogenic origin: the afferent pathway is in the somatic nerves, the efferent pathway in the sympathetic nerves.", "contents": "Neurogenic influences on blood pressure and vascular tone from peripheral receptors during muscular contraction. In anesthetized dogs, contraction of the muscles of the thigh was elicited either by applying electrodes in the muscles or by stimulation of the spinal ventral roots. The carotid sinuses were isolated; the vagi and the depressor nerves cut. Rhythmic muscular contraction caused hypotension and vasodilatation; tetanic contraction caused hypertension and vasoconstriction. These responses are of reflexogenic origin: the afferent pathway is in the somatic nerves, the efferent pathway in the sympathetic nerves.", "PMID": 975149}
{"id": "pubmed23n0032_10205", "title": "[Physiology of orthostatic regulation].", "content": "The arterial blood pressure is integrated into a feedback control system and practically depends on three parameters, i. e. blood volume, heart dynamics, and peripheral resistance. When circulation is disturbed in the orthostatic, the correcting elements heart and vessels have to adapt themselves according to this demand. By standing up, the hydrostatic pressure in the arterial vessels increases rapidly in the lower body regions. The initial increase of the hydrostatic pressure will be stopped by the valves in the venous system. By means of a higher arteriovenous pressure gradient the blood flow into the lower body regions increases more than 100%. The blood flow fills the venous system within 1-2 min successively from downward to upward and opens the valves. The venous pressure in the feet can rise to the full hydrostatic value of 100 mm Hg. This change of pressure causes a volume increase of approximately 200 cm3 regarding both legs of healthy persons. In this way the circulatory blood quantity is reduced. The heart is able to put out shortly an increased cardiac output by loading off the central blood volume (lung and end-systolic volume), in order to compensate orthostatic demand. After a long upright position the cardiac output is falling under the starting value and heart frequency increases. By sympathetic stimulation the peripheral resistance increases as well.", "contents": "[Physiology of orthostatic regulation]. The arterial blood pressure is integrated into a feedback control system and practically depends on three parameters, i. e. blood volume, heart dynamics, and peripheral resistance. When circulation is disturbed in the orthostatic, the correcting elements heart and vessels have to adapt themselves according to this demand. By standing up, the hydrostatic pressure in the arterial vessels increases rapidly in the lower body regions. The initial increase of the hydrostatic pressure will be stopped by the valves in the venous system. By means of a higher arteriovenous pressure gradient the blood flow into the lower body regions increases more than 100%. The blood flow fills the venous system within 1-2 min successively from downward to upward and opens the valves. The venous pressure in the feet can rise to the full hydrostatic value of 100 mm Hg. This change of pressure causes a volume increase of approximately 200 cm3 regarding both legs of healthy persons. In this way the circulatory blood quantity is reduced. The heart is able to put out shortly an increased cardiac output by loading off the central blood volume (lung and end-systolic volume), in order to compensate orthostatic demand. After a long upright position the cardiac output is falling under the starting value and heart frequency increases. By sympathetic stimulation the peripheral resistance increases as well.", "PMID": 975150}
{"id": "pubmed23n0032_10206", "title": "[Orthostatic immediate regulation].", "content": "A series of well-known orthostatic tests were performed in order to substantiate complaints of patients in respect to blood volume drawn into both legs as an early orthostatic regulation. It could be shown that the blood volume pooled in the legs changed highly from test to test up to 250%. There was evidence that with increasing stagnating blood volume, there was not only an increase of arterial blood influx to the lower extremities, but a maximum of heart rate as well as reaction to the orthostatic stress. Under standardized conditions, 14 orthostatically unstable subjects have been tested. 57% of them collapsed in the course of the tests. Following a 14-day administration of dihydroergotamine, the percentage of collapsing subjects was reduced to 7%. At the same time there was a decrease in the following simultaneously measured circulatory parameters as heart rate, mean arterial pressure and heart volume, especially in cases where these changes of parameters were significantly high at the beginning of the experiment. Suggestions are given when it is necessary to detect disturbance of early orthostatic regulation in patients subjected to orthostatic tests. The importance of the dicroty quotient of the arterial pulse as a diagnostic criterion is emphasized.", "contents": "[Orthostatic immediate regulation]. A series of well-known orthostatic tests were performed in order to substantiate complaints of patients in respect to blood volume drawn into both legs as an early orthostatic regulation. It could be shown that the blood volume pooled in the legs changed highly from test to test up to 250%. There was evidence that with increasing stagnating blood volume, there was not only an increase of arterial blood influx to the lower extremities, but a maximum of heart rate as well as reaction to the orthostatic stress. Under standardized conditions, 14 orthostatically unstable subjects have been tested. 57% of them collapsed in the course of the tests. Following a 14-day administration of dihydroergotamine, the percentage of collapsing subjects was reduced to 7%. At the same time there was a decrease in the following simultaneously measured circulatory parameters as heart rate, mean arterial pressure and heart volume, especially in cases where these changes of parameters were significantly high at the beginning of the experiment. Suggestions are given when it is necessary to detect disturbance of early orthostatic regulation in patients subjected to orthostatic tests. The importance of the dicroty quotient of the arterial pulse as a diagnostic criterion is emphasized.", "PMID": 975151}
{"id": "pubmed23n0032_10207", "title": "Autonomic influences and cardiac conduction in patients with sinus node disease.", "content": "18 patients with sinus bradycardia (8 SB) and tachycardia-bradycardia syndrome (10 TBS) were investigated using various vagan reflex manoeuvres, atrial pacing (AP), pharmacological tests and His bundle (HB) recordings, to assess autonomic influences, sinus node function and atrioventricular (AV) conduction. Three types of responses (R) were defined as normal (N), supernormal (SN), poor autonomic (PA). Poor reflex responses to vagal manoeuvres, performed in 12 patients, have no statistical significance. MxCSRT at AP suppression was measured in 13 patients: it was normal (less than or equal to 500 msec) in 8 and increased (from 680 to 4,200 msec) in 5 patients with appearance of junctional escapes. Moreover, AP suppression revealed a sinoatrial block (SAB) in 1 patient with normal MxCSRT. Isoprenaline, administered intravenously (3-6 mug) in 9 patients, showed 4 NR, 3 PAR und 2 SNR (unusual sinus tachycardia, 1; atrial fibrillation, 1). Atropine (0.5-1 mg), used in 15 patients, revealed 5 PAR and 1 SNR. Prostigmine (0.5 mg), used in 11 patients, induced 6 NR and 5 PAR. Lanatosid C (0.08 mg), administered in 16 patients, showed 10 NR, 5 PAR and 1 SNR. Finally, ajmaline (100 mg), given in 13 patients, revealed 5 junctional rhythms and one 2:1 SAB. HB recorded in 17 patients showed an increase of AH interval (greater than 140 msec) in 5 patients and a slight increase (60 msec) of HV interval in 2 patients. Three patients developed 2nd-degree AV block at paced rates greater than or equal to 110/min. Abnormal responses at AP suppression and reduced drug responses were noted in 5 patients suffering from Adam-Stokes attacks; a permanent pacemaker was inserted in 4 of these 5 patients. In conclusion, provocative tests, in 18 patients with SB or TBS, suggest a diffuse process disease involving the autonomic function of both sinus node and AV junction (11 patients) and the AV system (6 patients). These immediate results must be correlated with histological findings in the conduction system, as shown in one of our cases previously reported.", "contents": "Autonomic influences and cardiac conduction in patients with sinus node disease. 18 patients with sinus bradycardia (8 SB) and tachycardia-bradycardia syndrome (10 TBS) were investigated using various vagan reflex manoeuvres, atrial pacing (AP), pharmacological tests and His bundle (HB) recordings, to assess autonomic influences, sinus node function and atrioventricular (AV) conduction. Three types of responses (R) were defined as normal (N), supernormal (SN), poor autonomic (PA). Poor reflex responses to vagal manoeuvres, performed in 12 patients, have no statistical significance. MxCSRT at AP suppression was measured in 13 patients: it was normal (less than or equal to 500 msec) in 8 and increased (from 680 to 4,200 msec) in 5 patients with appearance of junctional escapes. Moreover, AP suppression revealed a sinoatrial block (SAB) in 1 patient with normal MxCSRT. Isoprenaline, administered intravenously (3-6 mug) in 9 patients, showed 4 NR, 3 PAR und 2 SNR (unusual sinus tachycardia, 1; atrial fibrillation, 1). Atropine (0.5-1 mg), used in 15 patients, revealed 5 PAR and 1 SNR. Prostigmine (0.5 mg), used in 11 patients, induced 6 NR and 5 PAR. Lanatosid C (0.08 mg), administered in 16 patients, showed 10 NR, 5 PAR and 1 SNR. Finally, ajmaline (100 mg), given in 13 patients, revealed 5 junctional rhythms and one 2:1 SAB. HB recorded in 17 patients showed an increase of AH interval (greater than 140 msec) in 5 patients and a slight increase (60 msec) of HV interval in 2 patients. Three patients developed 2nd-degree AV block at paced rates greater than or equal to 110/min. Abnormal responses at AP suppression and reduced drug responses were noted in 5 patients suffering from Adam-Stokes attacks; a permanent pacemaker was inserted in 4 of these 5 patients. In conclusion, provocative tests, in 18 patients with SB or TBS, suggest a diffuse process disease involving the autonomic function of both sinus node and AV junction (11 patients) and the AV system (6 patients). These immediate results must be correlated with histological findings in the conduction system, as shown in one of our cases previously reported.", "PMID": 975152}
{"id": "pubmed23n0032_10208", "title": "Cell cycle kinetics in an in vitro tumor model.", "content": "Cell cycle kinetic parameters of multicell spheroids in vitro have been estimated using thymidine labeling techniques and autoradiography. Both the mitotic index and thymidine labeling index decreased in larger spheroids, whereas the duration of the cell cycle, as determined by two independent methods utilizing labeled mitoses or labeled cells, was essentially independent of spheroid size or age. These results suggest that the tumor-like growth exhibited by spheroids is the result of a decreasing growth fraction and a large apparent cell loss, rather than a general elongation of the cell cycle.", "contents": "Cell cycle kinetics in an in vitro tumor model. Cell cycle kinetic parameters of multicell spheroids in vitro have been estimated using thymidine labeling techniques and autoradiography. Both the mitotic index and thymidine labeling index decreased in larger spheroids, whereas the duration of the cell cycle, as determined by two independent methods utilizing labeled mitoses or labeled cells, was essentially independent of spheroid size or age. These results suggest that the tumor-like growth exhibited by spheroids is the result of a decreasing growth fraction and a large apparent cell loss, rather than a general elongation of the cell cycle.", "PMID": 975203}
{"id": "pubmed23n0032_10209", "title": "Inner enamel epithelium cell production rates.", "content": "Crowning of all four rat incisors slows down their eruption rate, leading to a diminution of cell production by the inner enamel epithelium (IEE). The production rate by 100 progenitor cells is dominated as production rate %. The normal IEE in rats weighing 200 g produces daily 67 cells %. In the crowned incisor this rate drops to 15 cells % while during accelerated eruption rate and IEE cell production rate remains unchanged and an eruption of 6-4 mum reflects a production of 1 cell % by the IEE. A method which relates labelled cell displacement along the IEE with its cell production rate is described and its applicability to other cell renewal systems discussed.", "contents": "Inner enamel epithelium cell production rates. Crowning of all four rat incisors slows down their eruption rate, leading to a diminution of cell production by the inner enamel epithelium (IEE). The production rate by 100 progenitor cells is dominated as production rate %. The normal IEE in rats weighing 200 g produces daily 67 cells %. In the crowned incisor this rate drops to 15 cells % while during accelerated eruption rate and IEE cell production rate remains unchanged and an eruption of 6-4 mum reflects a production of 1 cell % by the IEE. A method which relates labelled cell displacement along the IEE with its cell production rate is described and its applicability to other cell renewal systems discussed.", "PMID": 975204}
{"id": "pubmed23n0032_10210", "title": "Kinetics of phytohemagglutinin stimulated lymphocytes in chronic lymphocytic leukemia.", "content": "The DNA synthesis pattern and several kinetic parameters of in vitro PHA stimulated normal and CLL lymphocytes were determined. The DNA synthesis peak of CLL lymphocytes occurred 2-3 days later than that of normal lymphocytes. The generation time, estimated by the labeled mitoses method, was found to be 28 hr and 20 hr for CLL and normal lymphocytes respectively. This difference was mainly due to longer S and G1 periods. It was also shown that both CLL and normal lymphocytes divide several times. These data were confirmed by the chromatid labeling pattern and by the halving of the grains and the double labeling techniques. By combining continuous and pulse labeling the growth fraction of CLL lymphocytes was found to be progressively increasing, because of the recruitment of new cells in cycle, from the third day of culture. Therefore the delayed peak of DNA synthesis of CLL lymphocytes was caused by a longer cell cycle and by a longer pre-replicative phase.", "contents": "Kinetics of phytohemagglutinin stimulated lymphocytes in chronic lymphocytic leukemia. The DNA synthesis pattern and several kinetic parameters of in vitro PHA stimulated normal and CLL lymphocytes were determined. The DNA synthesis peak of CLL lymphocytes occurred 2-3 days later than that of normal lymphocytes. The generation time, estimated by the labeled mitoses method, was found to be 28 hr and 20 hr for CLL and normal lymphocytes respectively. This difference was mainly due to longer S and G1 periods. It was also shown that both CLL and normal lymphocytes divide several times. These data were confirmed by the chromatid labeling pattern and by the halving of the grains and the double labeling techniques. By combining continuous and pulse labeling the growth fraction of CLL lymphocytes was found to be progressively increasing, because of the recruitment of new cells in cycle, from the third day of culture. Therefore the delayed peak of DNA synthesis of CLL lymphocytes was caused by a longer cell cycle and by a longer pre-replicative phase.", "PMID": 975205}
{"id": "pubmed23n0032_10211", "title": "Effect of various doses of estrogen to BALB/cCrgl neonatal female mice on mammary growth and branching at 5 weeks of age.", "content": "High doses of 17beta-estradiol given newborn mice the first 5 days of life had no effect on mammary growth at 3 weeks of age whereas glands measured at 5 weeks of age displayed marked dose-dependent differences in area and branching. The number of duct branches increased significantly as the estrogen dose was raised from the 25 mug threshold to 35 or to 70 mug. Ratios between area and branching remained constant under all experimental conditions. Regional, dose-dependent, differences in gland-pair response were also observed at 5 weeks. Mammary gland pairs differed significantly in several growth parameters. The third gland pair was smaller in area and had fewer branches, but the opposite was true of the second and fourth gland pairs. Systemic injection of high doses of 17beta-estradiol into neonates reduced differences in growth parameters between gland pairs.", "contents": "Effect of various doses of estrogen to BALB/cCrgl neonatal female mice on mammary growth and branching at 5 weeks of age. High doses of 17beta-estradiol given newborn mice the first 5 days of life had no effect on mammary growth at 3 weeks of age whereas glands measured at 5 weeks of age displayed marked dose-dependent differences in area and branching. The number of duct branches increased significantly as the estrogen dose was raised from the 25 mug threshold to 35 or to 70 mug. Ratios between area and branching remained constant under all experimental conditions. Regional, dose-dependent, differences in gland-pair response were also observed at 5 weeks. Mammary gland pairs differed significantly in several growth parameters. The third gland pair was smaller in area and had fewer branches, but the opposite was true of the second and fourth gland pairs. Systemic injection of high doses of 17beta-estradiol into neonates reduced differences in growth parameters between gland pairs.", "PMID": 975206}
{"id": "pubmed23n0032_10212", "title": "Extracellular stimulation of epidermal DNA synthesis.", "content": "The removal of the topmost cell layers of the epidermal stratum corneum by stripping initiates a series of biochemical events which alters the normal homeostatic control of and results in the acceleration of the cell cycle in basal cells which are ten to twenty cell layers removed from the site of stripping. One measure of accelerated events is a stimulation of thymidine incorporation into epidermal DNA at time intervals following stripping. Two peaks of maximal stimulation occur between 12 and 24 hr and 48 and 54 hr after stripping. Stimulation of thymidine incorporation into epidermal DNA by limited stripping is a useful technique for studying the stripping-initiated signal at the stratum corneum and its subsequent translation at the proliferative cell receptor site.", "contents": "Extracellular stimulation of epidermal DNA synthesis. The removal of the topmost cell layers of the epidermal stratum corneum by stripping initiates a series of biochemical events which alters the normal homeostatic control of and results in the acceleration of the cell cycle in basal cells which are ten to twenty cell layers removed from the site of stripping. One measure of accelerated events is a stimulation of thymidine incorporation into epidermal DNA at time intervals following stripping. Two peaks of maximal stimulation occur between 12 and 24 hr and 48 and 54 hr after stripping. Stimulation of thymidine incorporation into epidermal DNA by limited stripping is a useful technique for studying the stripping-initiated signal at the stratum corneum and its subsequent translation at the proliferative cell receptor site.", "PMID": 975207}
{"id": "pubmed23n0032_10213", "title": "Proliferation rate of haemopoietic stem cells after damage by several cytostatic agents.", "content": "The haemopoietic tissue of mice was damaged by different cell-cycle-stage specific and cell-cycle-stage non-specific cytostatic agents. The proliferation rate among the surviving pluripotential stem cells, i.e. those cells forming colonies in spleens of lethally irradiated mice (CFUs), was then investigated. The results suggest that, at least in the CFUs population, the cells which synthesize DNA in the S phase of the cell cycle inhibit the entry of the non-proliferating GO cells into cell cycle. This evidence was based on the ability of three cytostatic agents, hydroxyurea, cytosine arabinoside and methotrexate, which are toxic specifically to the S phase cells to increase the proliferation in the CFUs population. This increase was quite out of proportion to the small amount of damage they caused to the population. Colchicine, which kills cells in mitosis, and ionizing irradiation, damaging cells in all stages, proved to be much weaker stimulators of proliferation. It has been suggested that a mechanism for the control of cellular proliferation might be based on the negative feedback in the cell cycle. In this feedback control loop the cells which are preparing for cell division in the S phase of the cell cycle inhibit the entry of the non-proliferating GO cells into cell cycle.", "contents": "Proliferation rate of haemopoietic stem cells after damage by several cytostatic agents. The haemopoietic tissue of mice was damaged by different cell-cycle-stage specific and cell-cycle-stage non-specific cytostatic agents. The proliferation rate among the surviving pluripotential stem cells, i.e. those cells forming colonies in spleens of lethally irradiated mice (CFUs), was then investigated. The results suggest that, at least in the CFUs population, the cells which synthesize DNA in the S phase of the cell cycle inhibit the entry of the non-proliferating GO cells into cell cycle. This evidence was based on the ability of three cytostatic agents, hydroxyurea, cytosine arabinoside and methotrexate, which are toxic specifically to the S phase cells to increase the proliferation in the CFUs population. This increase was quite out of proportion to the small amount of damage they caused to the population. Colchicine, which kills cells in mitosis, and ionizing irradiation, damaging cells in all stages, proved to be much weaker stimulators of proliferation. It has been suggested that a mechanism for the control of cellular proliferation might be based on the negative feedback in the cell cycle. In this feedback control loop the cells which are preparing for cell division in the S phase of the cell cycle inhibit the entry of the non-proliferating GO cells into cell cycle.", "PMID": 975208}
{"id": "pubmed23n0032_10214", "title": "Autoradiographic study of type II-cell hyperplasia in lungs of mice chronicall exposed to urethane.", "content": "Hyperplasia of pulmonary alveolar epithelium was induced by exposure of adult mice to 0-1% urethane in drinking water, and their lungs were analysed using combined autoradiographic and morphometric methods. The response of pulmonary epithelium showed three phases: an initial one of 3 weeks in which the number of type II cells was consistently low and, after a transient decrease in 3H-thymidine labeling index, the latter steadily increased. Degenerating or dead type II cells were found, consequently this period was considered one in which cell death was disproportionately increased over cell production. Between the 3rd and 6th weeks the number of type II cells doubled, and thereafter increased at a slower rate. The 3H-thymidine labeling index reached its peak at 6 weeks. The third phase was marked by a decline in labeling index which returned to near-normal levels by the 16th week. The number of type II cells declined slowly after the 10th week and was still elevated at the end of the observation period. Tumors consisting of type II cells continued to arise even during the period of declining labeling index. The dissociation between the proliferative response which was reversible, and the neoplastic response which was not, supports the assumption that the major part of the observed population growth or hyperplasia induced by urethane was a form of regeneration repair secondary to cell death and may be unrelated to tumor growth.", "contents": "Autoradiographic study of type II-cell hyperplasia in lungs of mice chronicall exposed to urethane. Hyperplasia of pulmonary alveolar epithelium was induced by exposure of adult mice to 0-1% urethane in drinking water, and their lungs were analysed using combined autoradiographic and morphometric methods. The response of pulmonary epithelium showed three phases: an initial one of 3 weeks in which the number of type II cells was consistently low and, after a transient decrease in 3H-thymidine labeling index, the latter steadily increased. Degenerating or dead type II cells were found, consequently this period was considered one in which cell death was disproportionately increased over cell production. Between the 3rd and 6th weeks the number of type II cells doubled, and thereafter increased at a slower rate. The 3H-thymidine labeling index reached its peak at 6 weeks. The third phase was marked by a decline in labeling index which returned to near-normal levels by the 16th week. The number of type II cells declined slowly after the 10th week and was still elevated at the end of the observation period. Tumors consisting of type II cells continued to arise even during the period of declining labeling index. The dissociation between the proliferative response which was reversible, and the neoplastic response which was not, supports the assumption that the major part of the observed population growth or hyperplasia induced by urethane was a form of regeneration repair secondary to cell death and may be unrelated to tumor growth.", "PMID": 975209}
{"id": "pubmed23n0032_10215", "title": "On the intrinsic innervation of normal rat liver. Histochemical and scanning electron microscopical studies.", "content": "With the Bodian method stained fibers were observed in the lobules of the rat liver and with the modified Karnovsky and Roots thiocholine method cholinesterase (presumably acetylcholinesterase (AChE))-positive nerve fibers were found in a pattern similar to that of the Bodian-stained fibers. The AChE-positive nerve fibers form a network in the liver lobules in close relation to hepatocytes and sinusoids. Fluorescent varicose nerve fibers demonstrated by the glyoxylic acid and Falck-Hillarp fluorescence methods were found only in the interlobular spaces associated with vessels. As no overlapping of distribution patterns of AChE-positive nerve fibers and fluorescent nerve fibers occurs, the AChE activity of the nerves of the liver lobules probably reflects the associated presence of acetylcholine in the nerve fibers. In consequence we suggest that nerves of the liver lobules belong to the autonomic parasympathetic nervous system. SEM of liver tissue revealed light cords apparently situated in smooth-surfaced channels between adjacent hepatocytes and in the space of Disse, where fibers also cross sinusoids. We tentatively suggest that the cords of the SEM represent the AChE-positive nerve fibers of our LM observations.", "contents": "On the intrinsic innervation of normal rat liver. Histochemical and scanning electron microscopical studies. With the Bodian method stained fibers were observed in the lobules of the rat liver and with the modified Karnovsky and Roots thiocholine method cholinesterase (presumably acetylcholinesterase (AChE))-positive nerve fibers were found in a pattern similar to that of the Bodian-stained fibers. The AChE-positive nerve fibers form a network in the liver lobules in close relation to hepatocytes and sinusoids. Fluorescent varicose nerve fibers demonstrated by the glyoxylic acid and Falck-Hillarp fluorescence methods were found only in the interlobular spaces associated with vessels. As no overlapping of distribution patterns of AChE-positive nerve fibers and fluorescent nerve fibers occurs, the AChE activity of the nerves of the liver lobules probably reflects the associated presence of acetylcholine in the nerve fibers. In consequence we suggest that nerves of the liver lobules belong to the autonomic parasympathetic nervous system. SEM of liver tissue revealed light cords apparently situated in smooth-surfaced channels between adjacent hepatocytes and in the space of Disse, where fibers also cross sinusoids. We tentatively suggest that the cords of the SEM represent the AChE-positive nerve fibers of our LM observations.", "PMID": 975210}
{"id": "pubmed23n0032_10216", "title": "Localisation of proteins in coated micropinocytotic vesicles during transport across rabbit yolk sac endoderm.", "content": "Rabbit yolk sac splanchnopleur exposed in utero to IgG-HRP and IgG-ferritin conjugates, rabbit and bovine anti-HRP antibodies, free HRP, ferritin and human IgG, was examined ultrastructurally in an attempt to determine whether or not coated micropinocytotic vesicles are involved in selectively transporting immunoglobulins across yolk sac endodermal cells. Human, rabbit and bovine IgG-HRP conjugates, rabbit anti-HRP antibodies, free HRP and human IgG, all become localised in coated micropinocytotic vesicles. Differences were observed in that only human IgG and rabbit anti-HRP antibodies could be located in the intercellular space and bovine IgG-HRP conjugate could not be detected in coated micropinocytotic vesicles in confluence with the lateral and basal plasmalemma. Bovine anti-HRP anti-bodies, IgG-ferritin conjugates, and free ferritin, could not be observed in coated micropinocytotic vesicles. All proteins were detected in macropinocytotic vesicles, and dense bodies resembling phagolysosomes. Results are discussed in the light of a proposal that selection occurs at the cell surface during formation of coated micropinocytotic vesicles and is not linked to intracellular proteolysis.", "contents": "Localisation of proteins in coated micropinocytotic vesicles during transport across rabbit yolk sac endoderm. Rabbit yolk sac splanchnopleur exposed in utero to IgG-HRP and IgG-ferritin conjugates, rabbit and bovine anti-HRP antibodies, free HRP, ferritin and human IgG, was examined ultrastructurally in an attempt to determine whether or not coated micropinocytotic vesicles are involved in selectively transporting immunoglobulins across yolk sac endodermal cells. Human, rabbit and bovine IgG-HRP conjugates, rabbit anti-HRP antibodies, free HRP and human IgG, all become localised in coated micropinocytotic vesicles. Differences were observed in that only human IgG and rabbit anti-HRP antibodies could be located in the intercellular space and bovine IgG-HRP conjugate could not be detected in coated micropinocytotic vesicles in confluence with the lateral and basal plasmalemma. Bovine anti-HRP anti-bodies, IgG-ferritin conjugates, and free ferritin, could not be observed in coated micropinocytotic vesicles. All proteins were detected in macropinocytotic vesicles, and dense bodies resembling phagolysosomes. Results are discussed in the light of a proposal that selection occurs at the cell surface during formation of coated micropinocytotic vesicles and is not linked to intracellular proteolysis.", "PMID": 975211}
{"id": "pubmed23n0032_10217", "title": "Ultrastructure of the spermatozoa and spermiogenesis in New Zealand freshwater eels (Anguillidae).", "content": "The spermiogenesis and sperm ultrastructure of Anguilla australis schmidtii and A. dieffenbachii is described. During spermiogenesis the chromatin material condenses into the crescent-shaped head of the mature sperm. By elongation of the sperm head the mitochondrion is displaced to the anterior of the head remote from the posterior centriolar apparatus. A ciliary rootlet becomes segregated during spermiogenesis to form a membrane covered, striated, rod-like appendage. From the proximal centriole arise two sets of filaments, each containing three subfibrils. One group of five filaments extends anteriorly along the inner concave side of the head. A group of four modified filaments pass along the outer, convex, side. The middle subfibril B of each triplet in the group of four filaments is flattened and joins the two subfibrils A and B, forming a binocular shaped structure. The motile flagellum has a 9+0 axonemal pattern.", "contents": "Ultrastructure of the spermatozoa and spermiogenesis in New Zealand freshwater eels (Anguillidae). The spermiogenesis and sperm ultrastructure of Anguilla australis schmidtii and A. dieffenbachii is described. During spermiogenesis the chromatin material condenses into the crescent-shaped head of the mature sperm. By elongation of the sperm head the mitochondrion is displaced to the anterior of the head remote from the posterior centriolar apparatus. A ciliary rootlet becomes segregated during spermiogenesis to form a membrane covered, striated, rod-like appendage. From the proximal centriole arise two sets of filaments, each containing three subfibrils. One group of five filaments extends anteriorly along the inner concave side of the head. A group of four modified filaments pass along the outer, convex, side. The middle subfibril B of each triplet in the group of four filaments is flattened and joins the two subfibrils A and B, forming a binocular shaped structure. The motile flagellum has a 9+0 axonemal pattern.", "PMID": 975212}
{"id": "pubmed23n0032_10218", "title": "Structure and regeneration of the eyes of strombid gastropods.", "content": "The tips of the eyestalks of three species of strombid gastropods were amputated and the structure of the fully developed eye in investigated. The retina contains at least two types of cell: sensory cells bearing long tufts of microvilli with a central cytoplasmic core, and pigment cells with short microvilli. New eyes became visible at the tips of the eyestalk stump 5-16 days after amputation. When the regenerated eyes first appear, they consist of hollow balls of cells with a pigment lined cavity; two types of retinal cells are already distinguishable but their microvilli and cilia are small and sparse. The microvillous tufts and sensory cell contents develop quickly and about 14 days after their first appearance, the eye is a fully formed but miniature organ.", "contents": "Structure and regeneration of the eyes of strombid gastropods. The tips of the eyestalks of three species of strombid gastropods were amputated and the structure of the fully developed eye in investigated. The retina contains at least two types of cell: sensory cells bearing long tufts of microvilli with a central cytoplasmic core, and pigment cells with short microvilli. New eyes became visible at the tips of the eyestalk stump 5-16 days after amputation. When the regenerated eyes first appear, they consist of hollow balls of cells with a pigment lined cavity; two types of retinal cells are already distinguishable but their microvilli and cilia are small and sparse. The microvillous tufts and sensory cell contents develop quickly and about 14 days after their first appearance, the eye is a fully formed but miniature organ.", "PMID": 975213}
{"id": "pubmed23n0032_10219", "title": "Freeze-etching observations on the characteristic arrangement of intramembranous particles in the apical plasma membrane of the thyroid follicular cell in TSH-treated mice.", "content": "Thyroid glands of normal, TSH-treated and Thyradin (powdered thyroid)-treated mice were examined by means of the freeze-etching method. Intramembranous particles on the PF (= A face) face of the apical plasma membrane often form aggregates especially in TSH-treated mice. Each aggregate, about 200 nm in diameter, and consisting of 15-25 large particles, corresponds to a depression of the apical cytoplasm, and the particles sometimes form rosettes. Particle-aggregates are very rare in the apical plasma membrane of the thyroid follicular cell of the Thyradin-treated animal. In the cytoplasm just beneath the particle-aggregate no secretory granules, reabsorbed colloid droplets or other special structures are found. From these facts, the aggregate is considered closely related to an initial site for the micropinocytosis of the luminal colloid.", "contents": "Freeze-etching observations on the characteristic arrangement of intramembranous particles in the apical plasma membrane of the thyroid follicular cell in TSH-treated mice. Thyroid glands of normal, TSH-treated and Thyradin (powdered thyroid)-treated mice were examined by means of the freeze-etching method. Intramembranous particles on the PF (= A face) face of the apical plasma membrane often form aggregates especially in TSH-treated mice. Each aggregate, about 200 nm in diameter, and consisting of 15-25 large particles, corresponds to a depression of the apical cytoplasm, and the particles sometimes form rosettes. Particle-aggregates are very rare in the apical plasma membrane of the thyroid follicular cell of the Thyradin-treated animal. In the cytoplasm just beneath the particle-aggregate no secretory granules, reabsorbed colloid droplets or other special structures are found. From these facts, the aggregate is considered closely related to an initial site for the micropinocytosis of the luminal colloid.", "PMID": 975214}
{"id": "pubmed23n0032_10220", "title": "Gap junctions in hemodichorial and hemotrichorial placentae.", "content": "Gap junctions were found to be a constant feature of chorioallantoic placentae with two or three trophoblastic layers. The gap junctions connect layers I and II in hemodichorial and layers II and III in hemotrichorial placentae. Although the gap junctions vary in form and in the packing density of membrane-associated particles, they cover an extensive surface area in all species examined. The gap junctions always connect adjacent membranes of two trophoblastic layers, which show no evidence of micropinocytotic activity; at least one of these trophoblastic layers is syncytial. It is therefore concluded that the gap junctions play an important role in diaplacental transport. We consider that gap junctions act as molecular sieves, resulting in limitations in the transport of large molecules. The passage of small molecules, on the contrary, would be facilitated by the gap junctions.", "contents": "Gap junctions in hemodichorial and hemotrichorial placentae. Gap junctions were found to be a constant feature of chorioallantoic placentae with two or three trophoblastic layers. The gap junctions connect layers I and II in hemodichorial and layers II and III in hemotrichorial placentae. Although the gap junctions vary in form and in the packing density of membrane-associated particles, they cover an extensive surface area in all species examined. The gap junctions always connect adjacent membranes of two trophoblastic layers, which show no evidence of micropinocytotic activity; at least one of these trophoblastic layers is syncytial. It is therefore concluded that the gap junctions play an important role in diaplacental transport. We consider that gap junctions act as molecular sieves, resulting in limitations in the transport of large molecules. The passage of small molecules, on the contrary, would be facilitated by the gap junctions.", "PMID": 975215}
{"id": "pubmed23n0032_10221", "title": "Structure and function of prothoracic glands and oenocytes in embryos and last larval instars of Oncopeltus fasciatus Dallas (Insecta, Heteroptera).", "content": "1. Active prothoracic glands and oenocytes of last larval stage are both characteristized by well-developed smooth and rough endoplasmic reticulum (ER). Prothoracic glands also show plasma membrane infoldings, but not oenocytes which contain a large number of pleomorphic vesicles. 2. The fine structure of embryonic oenocytes corresponds after blastokinesis with that of active larval and adult cells. Thus, an activity in the late embryo can be assumed. Embryonic prothoracic glands reveal no signs of activity: smooth and rough ER are absent. The subcellular structure resembles that of organ anlagen, i.e. not yet fully differentiated tissue. Hormone synthesis is not likely. 3. Ecdysone titer was determined throughout embryonic development and in mature adults. Although prothoracic glands break down during adult ecdysis, imagines contain in the Calliphora-bioassay active factors: females 0.9 CU/g and males 0.5 CU/g. As sites of synthesis the oenocytes are suggested. 4. A relatively high ecdysone titer of 7 CU/g is measured in newly deposited eggs. The hormone is presumably of maternal origin. Subsequent to blastokinesis the hormone content increases dramatically up to about 180 CU/g, apparently due to endocrine function of the embryo. Oenocytes are proposed as the source of ecdysone during late embryonic development. 5. The function of ecdysone during early and advanced embryogenesis, especially in view of \"embryonic molts\", is discussed.", "contents": "Structure and function of prothoracic glands and oenocytes in embryos and last larval instars of Oncopeltus fasciatus Dallas (Insecta, Heteroptera). 1. Active prothoracic glands and oenocytes of last larval stage are both characteristized by well-developed smooth and rough endoplasmic reticulum (ER). Prothoracic glands also show plasma membrane infoldings, but not oenocytes which contain a large number of pleomorphic vesicles. 2. The fine structure of embryonic oenocytes corresponds after blastokinesis with that of active larval and adult cells. Thus, an activity in the late embryo can be assumed. Embryonic prothoracic glands reveal no signs of activity: smooth and rough ER are absent. The subcellular structure resembles that of organ anlagen, i.e. not yet fully differentiated tissue. Hormone synthesis is not likely. 3. Ecdysone titer was determined throughout embryonic development and in mature adults. Although prothoracic glands break down during adult ecdysis, imagines contain in the Calliphora-bioassay active factors: females 0.9 CU/g and males 0.5 CU/g. As sites of synthesis the oenocytes are suggested. 4. A relatively high ecdysone titer of 7 CU/g is measured in newly deposited eggs. The hormone is presumably of maternal origin. Subsequent to blastokinesis the hormone content increases dramatically up to about 180 CU/g, apparently due to endocrine function of the embryo. Oenocytes are proposed as the source of ecdysone during late embryonic development. 5. The function of ecdysone during early and advanced embryogenesis, especially in view of \"embryonic molts\", is discussed.", "PMID": 975216}
{"id": "pubmed23n0032_10222", "title": "The first optic ganglion of the bee. II. Topographical relationships of the monopolar cells within and between cartridges.", "content": "The arrangement of first and second order neurons in an optic cartridge and the topographical relationships of the second order neurons within a cartridge and to groups of surrounding cartridges have been analyzed in the visual system of the bee, Apis mellifera, from light and electron microscope studies on Golgi preparations. At the level of the monopolar cell body layer, the nine retinula cell fibres of each ommatidium, the six short visual fibres arranged in a circle surrounding the three long visual fibres, become cartridges as a consequence of the appearance of the second order neurons (L-fibres) which join the R-fibre bundles. Two of the four different L-fibre types, L-1 and L-2, remain together in the centre of the cartridge throughout the lamina. The axons of the L-3 and L-4 fibres, however, have their position integrated into the circle formed by the endings of the short visual fibres. On the basis of further examination of light and especially electron microscopical Golgi material, the different L-fibres can be classified into four types which appear in each cartridge. The clear stratification in the first synaptic region (A, B and C) seems to be the best criterion for a morphological classification since such a classification necessarily also includes a functional basis. According to a naming system based on the position of the lateral processes, L-fibres with side branches in strata A, B and C are called L-1 fibres. Fibres with lateral processes in strata A and B are L-2 fibres; monopolar cell fibres with branches only in the second stratum B are L-fibres of type 3; and all monopolar cells with branches only in stratum C are called L-4 fibres. In addition to the branching pattern covering only the parent cartridge, two of the four fibre types (L-2 and L-4) have long collaterals reaching neighbouring cartridges: L-2 in stratum A and L-4 in straum C. These collaterals presumably form a substrate for lateral interactions.", "contents": "The first optic ganglion of the bee. II. Topographical relationships of the monopolar cells within and between cartridges. The arrangement of first and second order neurons in an optic cartridge and the topographical relationships of the second order neurons within a cartridge and to groups of surrounding cartridges have been analyzed in the visual system of the bee, Apis mellifera, from light and electron microscope studies on Golgi preparations. At the level of the monopolar cell body layer, the nine retinula cell fibres of each ommatidium, the six short visual fibres arranged in a circle surrounding the three long visual fibres, become cartridges as a consequence of the appearance of the second order neurons (L-fibres) which join the R-fibre bundles. Two of the four different L-fibre types, L-1 and L-2, remain together in the centre of the cartridge throughout the lamina. The axons of the L-3 and L-4 fibres, however, have their position integrated into the circle formed by the endings of the short visual fibres. On the basis of further examination of light and especially electron microscopical Golgi material, the different L-fibres can be classified into four types which appear in each cartridge. The clear stratification in the first synaptic region (A, B and C) seems to be the best criterion for a morphological classification since such a classification necessarily also includes a functional basis. According to a naming system based on the position of the lateral processes, L-fibres with side branches in strata A, B and C are called L-1 fibres. Fibres with lateral processes in strata A and B are L-2 fibres; monopolar cell fibres with branches only in the second stratum B are L-fibres of type 3; and all monopolar cells with branches only in stratum C are called L-4 fibres. In addition to the branching pattern covering only the parent cartridge, two of the four fibre types (L-2 and L-4) have long collaterals reaching neighbouring cartridges: L-2 in stratum A and L-4 in straum C. These collaterals presumably form a substrate for lateral interactions.", "PMID": 975217}
{"id": "pubmed23n0032_10223", "title": "Fine structure of the corpus luteum of the autografted ovary in the rat.", "content": "The rat ovary has been transplanted successfully to subcutaneous tissue areas by several investigators. Light microscopy has revealed that corpora lutea in ovarian autografts are formed by luteinization of intact follicles and contain entrapped ova. In the present study, corpora lutea from autografted ovaries in castrate rats were obtained at metestrus and examined electron microscopically to determine whether their cellular morphology correlated with the normal progesterone levels in these animals. Cellular features usually accepted as regressive were apparent. The findings suggest either structural luteolysis is occurring before functional luteolysis or that the adrenal has increased steroidogenic activity in the castrate with ovarian autografts to account for the normal progesterone levels.", "contents": "Fine structure of the corpus luteum of the autografted ovary in the rat. The rat ovary has been transplanted successfully to subcutaneous tissue areas by several investigators. Light microscopy has revealed that corpora lutea in ovarian autografts are formed by luteinization of intact follicles and contain entrapped ova. In the present study, corpora lutea from autografted ovaries in castrate rats were obtained at metestrus and examined electron microscopically to determine whether their cellular morphology correlated with the normal progesterone levels in these animals. Cellular features usually accepted as regressive were apparent. The findings suggest either structural luteolysis is occurring before functional luteolysis or that the adrenal has increased steroidogenic activity in the castrate with ovarian autografts to account for the normal progesterone levels.", "PMID": 975218}
{"id": "pubmed23n0032_10224", "title": "Fine structure of nerve-melanophore contacts in the angelfish Pterophyllum scalare.", "content": "Contacts between small unmyelinated nerve fibres and dermal melanophores of the angelfish, Pterophyllum scalare, exhibit several features characteristic of synapses, including small synpatic vesicles and dense core vesicles, a narrow synaptic cleft, electron-dense material at the postsynaptic membrane (cell membrane of the melanophore) and, occasionally, presynaptic densities. An analysis of serial thin sections shows that the synapses described here represent varicosities of an otherwise more or less straight nerve fibre. A single axon thereby may form several en passant synapses with a single melanophore. It is suggested that the synaptic contacts described here not only represent sites of transmitter release but also play a role as sites of firm attachment between nerves and melanophores which guarantee a stable arrangement of nerve fibres and melanophores.", "contents": "Fine structure of nerve-melanophore contacts in the angelfish Pterophyllum scalare. Contacts between small unmyelinated nerve fibres and dermal melanophores of the angelfish, Pterophyllum scalare, exhibit several features characteristic of synapses, including small synpatic vesicles and dense core vesicles, a narrow synaptic cleft, electron-dense material at the postsynaptic membrane (cell membrane of the melanophore) and, occasionally, presynaptic densities. An analysis of serial thin sections shows that the synapses described here represent varicosities of an otherwise more or less straight nerve fibre. A single axon thereby may form several en passant synapses with a single melanophore. It is suggested that the synaptic contacts described here not only represent sites of transmitter release but also play a role as sites of firm attachment between nerves and melanophores which guarantee a stable arrangement of nerve fibres and melanophores.", "PMID": 975219}
{"id": "pubmed23n0032_10225", "title": "Shell attachment in the pond snail Lymnaea stagnalis (L.)", "content": "The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the epidermis, the adhesive epithelium. The cells of this epithelium are filled with microfilaments and possess characteristic knob-like microvilli. The epithelium is attached to the sheel by way of an adhesive substance containing proteins and mucopolysaccharides.", "contents": "Shell attachment in the pond snail Lymnaea stagnalis (L.). The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the epidermis, the adhesive epithelium. The cells of this epithelium are filled with microfilaments and possess characteristic knob-like microvilli. The epithelium is attached to the sheel by way of an adhesive substance containing proteins and mucopolysaccharides.", "PMID": 975220}
{"id": "pubmed23n0032_10226", "title": "Direct innervation of the mantle edge gland by neurosecretory axons in Helisoma duryi (Mollusca: Pulmonata).", "content": "The mantle edge gland of Helisoma duryi is innervated by neurosecretory axons from the pallial nerves. Synaptoid contacts occur between axons and gland cells, and there is ultrastructural evidence for the release of neurosecretory material. The mantle edge gland contributes to the deposition of periostracum during shell formation, and direct neurosecretory innervation may control shell growth and regeneration.", "contents": "Direct innervation of the mantle edge gland by neurosecretory axons in Helisoma duryi (Mollusca: Pulmonata). The mantle edge gland of Helisoma duryi is innervated by neurosecretory axons from the pallial nerves. Synaptoid contacts occur between axons and gland cells, and there is ultrastructural evidence for the release of neurosecretory material. The mantle edge gland contributes to the deposition of periostracum during shell formation, and direct neurosecretory innervation may control shell growth and regeneration.", "PMID": 975221}
{"id": "pubmed23n0032_10227", "title": "Myelinated synapse-bearing cell bodies in the central nervous system of Scyliorhinus canicula (L.)", "content": "Some neurons of the anterior lateral-line lobe of dogfish (Scyliorhinus) have synapse-bearing perikarya enclosed by layers of compact myelin. The identity of these cells, which have myelinated axons and unmyelinated, synapse-bearing dendrites, is unknown.", "contents": "Myelinated synapse-bearing cell bodies in the central nervous system of Scyliorhinus canicula (L.). Some neurons of the anterior lateral-line lobe of dogfish (Scyliorhinus) have synapse-bearing perikarya enclosed by layers of compact myelin. The identity of these cells, which have myelinated axons and unmyelinated, synapse-bearing dendrites, is unknown.", "PMID": 975222}
{"id": "pubmed23n0032_10228", "title": "Effect of cholecystokinin-pancreozymin (CCK-PZ) on glycoprotein secretion from mouse gallbladder epithelium: an ultrastructural and cytochemical study.", "content": "Structural changes in the gallbladder epithelial cells of the mouse were studied following in vivo and in vitro stimulation of the gallbladder with the gastrointestinal hormone cholecystokinin-pancreozymin (CCK-PZ). Signs of increased secretory activity were observed within the first 2-3 min after hormone administration. At the ultrastructural level, best visualized with the PA-CrA-silver technique, granule discharge was observed, as was an overall increase in size of the granules. After prolonged in vitro incubation or repeated in vivo stimulation, there was an almost total depletion of secretory granules. This phenomenon is accompanied by an enhanced uptake of extracellular thorium dioxide by endocytotic vesicles at the apical cell surface. An exocytosis-endocytosis coupling mechanism may be important for membrane conservation in the gallbladder epithelial cells. The findings establish that the hormone CCK-PZ stimulates the secretion of glycoproteins from the mouse gallbladder epithelium.", "contents": "Effect of cholecystokinin-pancreozymin (CCK-PZ) on glycoprotein secretion from mouse gallbladder epithelium: an ultrastructural and cytochemical study. Structural changes in the gallbladder epithelial cells of the mouse were studied following in vivo and in vitro stimulation of the gallbladder with the gastrointestinal hormone cholecystokinin-pancreozymin (CCK-PZ). Signs of increased secretory activity were observed within the first 2-3 min after hormone administration. At the ultrastructural level, best visualized with the PA-CrA-silver technique, granule discharge was observed, as was an overall increase in size of the granules. After prolonged in vitro incubation or repeated in vivo stimulation, there was an almost total depletion of secretory granules. This phenomenon is accompanied by an enhanced uptake of extracellular thorium dioxide by endocytotic vesicles at the apical cell surface. An exocytosis-endocytosis coupling mechanism may be important for membrane conservation in the gallbladder epithelial cells. The findings establish that the hormone CCK-PZ stimulates the secretion of glycoproteins from the mouse gallbladder epithelium.", "PMID": 975223}
{"id": "pubmed23n0032_10229", "title": "Thyroid gland in dwarf mice: a stereological study.", "content": "Stereological methods were used to compare thyroids of dwarf mice and of their heterozygote littermates. In the thyroid of dwarf mice unorganized cellular masses, adipous tissue and ultimobranchial cysts are abundant. Follicles are small and their distribution function is unimodal. The number of cells per follicle is considerably lowered if compared with the normal. In control mice the distribution function of thyroid follicles is bimodal. These data show that origin of the thyroid anomaly in dwarf mice is due to a drastic diminution of cell divisions, probably resulting from the lack of growth hormone.", "contents": "Thyroid gland in dwarf mice: a stereological study. Stereological methods were used to compare thyroids of dwarf mice and of their heterozygote littermates. In the thyroid of dwarf mice unorganized cellular masses, adipous tissue and ultimobranchial cysts are abundant. Follicles are small and their distribution function is unimodal. The number of cells per follicle is considerably lowered if compared with the normal. In control mice the distribution function of thyroid follicles is bimodal. These data show that origin of the thyroid anomaly in dwarf mice is due to a drastic diminution of cell divisions, probably resulting from the lack of growth hormone.", "PMID": 975224}
{"id": "pubmed23n0032_10230", "title": "Estrogen dependent ciliogenesis in the chick oviduct.", "content": "Both the hormone dependency and the morphological details of estrogen-dependent ciliogenesis in the shell gland of the chick oviduct were investigated. Ciliogenesis was initiated on day 3 of estrogen treatment, and progressively more cells became differentiated until, on day 10, approximately 55% ciliation occurred with 17 beta-estradiol (1 mg/day) and approximately 75% ciliation occurred with diethylstilbestrol (1 mg/day). Simultaneous administration of progesterone with diethylstilbestrol (1 mg each/day for 10 days) caused a 50% depression in the number of ciliated cells on day 10. The rate of ciliogenesis was found to be affected by progesterone and the type of estrogen administered. The minimum stimulatory dose of estradiol was found to be between 0.01 mg/day and 0.05 mg/day. Ciliogenic cells were first recognized by the appearance of pro-basal bodies in the apical portion of the cell. Pro-basal body maturation and cilium formation were the same as those described for the chick trachea. Ciliogenesis in the chick was found to be homologous to estrogen-dependent ciliogenesis in various mammalian oviducts.", "contents": "Estrogen dependent ciliogenesis in the chick oviduct. Both the hormone dependency and the morphological details of estrogen-dependent ciliogenesis in the shell gland of the chick oviduct were investigated. Ciliogenesis was initiated on day 3 of estrogen treatment, and progressively more cells became differentiated until, on day 10, approximately 55% ciliation occurred with 17 beta-estradiol (1 mg/day) and approximately 75% ciliation occurred with diethylstilbestrol (1 mg/day). Simultaneous administration of progesterone with diethylstilbestrol (1 mg each/day for 10 days) caused a 50% depression in the number of ciliated cells on day 10. The rate of ciliogenesis was found to be affected by progesterone and the type of estrogen administered. The minimum stimulatory dose of estradiol was found to be between 0.01 mg/day and 0.05 mg/day. Ciliogenic cells were first recognized by the appearance of pro-basal bodies in the apical portion of the cell. Pro-basal body maturation and cilium formation were the same as those described for the chick trachea. Ciliogenesis in the chick was found to be homologous to estrogen-dependent ciliogenesis in various mammalian oviducts.", "PMID": 975225}
{"id": "pubmed23n0032_10231", "title": "The subacrosomal granule and its evolution during spermiogenesis in a lizard. Observations about the acrosomal fringe and the spermatid-sertoli cell relationship.", "content": "Some aspects of spermiogenesis have been studied in the testis of the teiid lizard Cnemidophorus lemniscatus lemniscatus by electron microscopy. Shortly after the acrosomal vesicle is lodged in a nuclear concavity of the spermatid, a dense granule differentiates in the center of the subacrosomal space. It is cone-shaped and shows a longitudinal striation. Its base applies to the acrosomal membrane and, through this, to the acrosomal granule. Its rounded vertex causes a depression of the nuclear membranes which, initially juxtaposed, separates at this point to form a vesicle. The granule develops and becomes a rod when spermiogenesis is advanced and the subacrosomal space has taken the form of a secondary cap. The rod is cylindrical, retains its original striation and has a convex acrosomal end. It encloses the vesicle formed by the nuclear envelope in its base and follows the apex of the nucleus. Meanwhile, the acrosomal granule loses its identity and the acrosomal cap is filled with a dense substance, in which a fringe of translucent material differentiates. This fringe lies in the dorsal and apical margins of the acrosome and is incompletely divided by longitudinal crests of the dense acrosomal substance. A projection of the Sertoli cell forms an accessory cap which envelops the acrosome and is in turn covered by the cytoplasm of the spermatid, constituting an intricate association. Two reflex membranes underlie the plasmalemma in the outer surface of the projection of the Sertoli cell. They are continuous with one another at their ends and with the cell membrane in the edge of pores. In the peripheral cytoplasm of the spermatid facing the accessory cap, numerous microtubules run longitudinally. By means of thin membranes some are interconnected or connected with the plasmalemma, from which they seem to originate.", "contents": "The subacrosomal granule and its evolution during spermiogenesis in a lizard. Observations about the acrosomal fringe and the spermatid-sertoli cell relationship. Some aspects of spermiogenesis have been studied in the testis of the teiid lizard Cnemidophorus lemniscatus lemniscatus by electron microscopy. Shortly after the acrosomal vesicle is lodged in a nuclear concavity of the spermatid, a dense granule differentiates in the center of the subacrosomal space. It is cone-shaped and shows a longitudinal striation. Its base applies to the acrosomal membrane and, through this, to the acrosomal granule. Its rounded vertex causes a depression of the nuclear membranes which, initially juxtaposed, separates at this point to form a vesicle. The granule develops and becomes a rod when spermiogenesis is advanced and the subacrosomal space has taken the form of a secondary cap. The rod is cylindrical, retains its original striation and has a convex acrosomal end. It encloses the vesicle formed by the nuclear envelope in its base and follows the apex of the nucleus. Meanwhile, the acrosomal granule loses its identity and the acrosomal cap is filled with a dense substance, in which a fringe of translucent material differentiates. This fringe lies in the dorsal and apical margins of the acrosome and is incompletely divided by longitudinal crests of the dense acrosomal substance. A projection of the Sertoli cell forms an accessory cap which envelops the acrosome and is in turn covered by the cytoplasm of the spermatid, constituting an intricate association. Two reflex membranes underlie the plasmalemma in the outer surface of the projection of the Sertoli cell. They are continuous with one another at their ends and with the cell membrane in the edge of pores. In the peripheral cytoplasm of the spermatid facing the accessory cap, numerous microtubules run longitudinally. By means of thin membranes some are interconnected or connected with the plasmalemma, from which they seem to originate.", "PMID": 975226}
{"id": "pubmed23n0032_10232", "title": "Differences in black pigmentation in lepidopteran cuticles as revealed by light and electron microscopy.", "content": "Black cuticles of larvae and pupae from various Lepidoptera were studied by light and electron microscopy. There are striking differences in the representation of black pigmentation, especially at the ultrastructural level. Two types may be described: 1. With the light microscope black melanin-like grana, electron-dense electron microscopically, are found in the distal parts of the exocuticle. This type is demonstrated in larvae of Celerio euphorbiae, Papilio machaon, and Phalera bucephala. 2. With the light microscope, a dark homogeneous layer in the distal exocuticle can be recognized, however, electron microscopically no structures correlated with this dark pigment layer. This type of pigmentation was present in pupae of Pieris brassicae and Aglais urticae; in Pieris larvae the dark pigmented layer appeared to be limited to the epicuticle. In Celerio processes of the epidermal cells are involved in transporting precursors to the exocuticle. The conclusion was reached that black pigmentation in cuticles is based on different mechanisms as proposed by structural features. The two likely mechanisms are melanization and sclerotization.", "contents": "Differences in black pigmentation in lepidopteran cuticles as revealed by light and electron microscopy. Black cuticles of larvae and pupae from various Lepidoptera were studied by light and electron microscopy. There are striking differences in the representation of black pigmentation, especially at the ultrastructural level. Two types may be described: 1. With the light microscope black melanin-like grana, electron-dense electron microscopically, are found in the distal parts of the exocuticle. This type is demonstrated in larvae of Celerio euphorbiae, Papilio machaon, and Phalera bucephala. 2. With the light microscope, a dark homogeneous layer in the distal exocuticle can be recognized, however, electron microscopically no structures correlated with this dark pigment layer. This type of pigmentation was present in pupae of Pieris brassicae and Aglais urticae; in Pieris larvae the dark pigmented layer appeared to be limited to the epicuticle. In Celerio processes of the epidermal cells are involved in transporting precursors to the exocuticle. The conclusion was reached that black pigmentation in cuticles is based on different mechanisms as proposed by structural features. The two likely mechanisms are melanization and sclerotization.", "PMID": 975227}
{"id": "pubmed23n0032_10233", "title": "Fine structure of the ocellus of the cabbage looper moth (Trichoplusia ni).", "content": "The dorsal ocelli of adult cabbage looper moths were studied by light microscopy, and scanning and transmission electron microscopy. Each ocellus has a cuticular lens located on the distal end of a cuticular cone which encapsulates the receptor cells. There are two distinct types of receptor cells in the ocellus. Seventy large receptor cells from plate-like rhabdoms with several adjacent cells to produce a rhabdom network in the ocellus. Proximally ninety small receptor cells which have a disorganized microvillar rhabdomere are located at the base of the rhabdoms formed by the large cells. Extensive areas of gap junctions which occur between the rhabdoms and the membranes of large and small cells suggest that the cells may be electrically coupled to one another. Axons from both large and small receptor cells leave the base of the ocellus and extend to the protocerebrum to synapse with second order neurons.", "contents": "Fine structure of the ocellus of the cabbage looper moth (Trichoplusia ni). The dorsal ocelli of adult cabbage looper moths were studied by light microscopy, and scanning and transmission electron microscopy. Each ocellus has a cuticular lens located on the distal end of a cuticular cone which encapsulates the receptor cells. There are two distinct types of receptor cells in the ocellus. Seventy large receptor cells from plate-like rhabdoms with several adjacent cells to produce a rhabdom network in the ocellus. Proximally ninety small receptor cells which have a disorganized microvillar rhabdomere are located at the base of the rhabdoms formed by the large cells. Extensive areas of gap junctions which occur between the rhabdoms and the membranes of large and small cells suggest that the cells may be electrically coupled to one another. Axons from both large and small receptor cells leave the base of the ocellus and extend to the protocerebrum to synapse with second order neurons.", "PMID": 975228}
{"id": "pubmed23n0032_10234", "title": "Manganese in the shell of Centropyxis (Rhizopodea: Protozoa).", "content": "The proteinaceous shells of Centropyxis hirsuta contain a relatively high concentration of manganese in an amorphous state. The concentrations of manganese fill the alveoli which are characteristic of the shell structure. Observations based on cultured animals and subsequently examined by X-ray diffraction, a solid state energy dispersive X-ray analyser, and an analytical electron microscope, lead to the conclusions that manganese is selectively absorbed by Centropyxis hirsuta and deposited in the shell.", "contents": "Manganese in the shell of Centropyxis (Rhizopodea: Protozoa). The proteinaceous shells of Centropyxis hirsuta contain a relatively high concentration of manganese in an amorphous state. The concentrations of manganese fill the alveoli which are characteristic of the shell structure. Observations based on cultured animals and subsequently examined by X-ray diffraction, a solid state energy dispersive X-ray analyser, and an analytical electron microscope, lead to the conclusions that manganese is selectively absorbed by Centropyxis hirsuta and deposited in the shell.", "PMID": 975229}
{"id": "pubmed23n0032_10235", "title": "Characterization of the 5' termini of hn RNA in mouse L cells: implications for processing and cap formation.", "content": "An analysis of the phosphorylated and capped 5' termini of the heterogenous nuclear RNA of mouse L cells has revealed four types of structure: pppXp..., ppXp..., pXp..., and m7/GpppXmp.... The 5'triphosphate termini consists exclusively of pppGp... and pppAp..., whereas a large proportion of the 5' monophosphate termini are pUp.... The 5'diphosphate termini contain all four species of nucleotide in relative proportions that are roughly similar to those found at the Xm position of cap structures. These results indicate that initiation of hnRNA transcription occurre exclusively with purine nucleotides, and consequently that the hnRNA molecules containing pyrimidines at the 5' termini very probably arise by cleavages at internal sites of larger primary transcripts. Taken together with previous results relating cap structures of hnRNA and mRNA, the data favor a model in which some mRNA sequences are located at transcriptionally initiated proportions and others in internal regions of their precursors. According to this model, both the mRNA segments derived from initial 5' end, and those derived by cleavage at internal sites could be converted to diphosphate-terminated derivatives, which then condense with GTP to form cap structures according to the mechanism previously described for vaccinia and reovirus mRNA.", "contents": "Characterization of the 5' termini of hn RNA in mouse L cells: implications for processing and cap formation. An analysis of the phosphorylated and capped 5' termini of the heterogenous nuclear RNA of mouse L cells has revealed four types of structure: pppXp..., ppXp..., pXp..., and m7/GpppXmp.... The 5'triphosphate termini consists exclusively of pppGp... and pppAp..., whereas a large proportion of the 5' monophosphate termini are pUp.... The 5'diphosphate termini contain all four species of nucleotide in relative proportions that are roughly similar to those found at the Xm position of cap structures. These results indicate that initiation of hnRNA transcription occurre exclusively with purine nucleotides, and consequently that the hnRNA molecules containing pyrimidines at the 5' termini very probably arise by cleavages at internal sites of larger primary transcripts. Taken together with previous results relating cap structures of hnRNA and mRNA, the data favor a model in which some mRNA sequences are located at transcriptionally initiated proportions and others in internal regions of their precursors. According to this model, both the mRNA segments derived from initial 5' end, and those derived by cleavage at internal sites could be converted to diphosphate-terminated derivatives, which then condense with GTP to form cap structures according to the mechanism previously described for vaccinia and reovirus mRNA.", "PMID": 975236}
{"id": "pubmed23n0032_10236", "title": "Comparative organization of active transcription units in Oncopeltus fasciatus.", "content": "We have analyzed electron micrographs of chromatin-associated fiber arrays from embryos of the milkweed bug, Oncopeltus fasciatus. The analysis has revealed that the arrays have highly ordered patterns of fiber spacings and lengths. These patterns support the interpretation that the fibers are nascent RNA with associated proteins (RNP fibers) which have resulted from transcription of the DNA in the underlying chromatin segment. In particular, the patterns indicate that the chromatin underlying each array is delimited by specific sites for initiation and termination of transcription. We apply the term transcription unit to a chromatin segment thus bounded. The analysis has further revealed that transcription units can be grouped into two principal classes--ribosomal and nonribosomal. Active transcription units of these two classes differ in DNA content, in their proximity to other active transcription units, and in their chromatin morphology. For certain developmental stages, fiber frequencies (that is, the nubmers of fibers per mum of chromatin) are also useful in distinguishing ribosomal from nonribosomal arrays. The most definitive of the above classification criteria is chromatin morphology as observed under our preparative conditions. We propose that term rho chromatin for the unbeaded or smooth chromatin that underlies nascent ribosomal RNP fibers. DNA in rho chromatin has a calculated packing ratio of approximately 1.2 mum of B structure DNA per mum of chromatin. Nu chromatin is used to designate the beaded chromatin for which we calculate a DNA packing ratio of 1.6-2.3 in our preparations. This calculation for nu chromatin is based on the inference that the beads are nucleosomes (nu bodies, PS particles, unit particles). The beaded morphology is observed between fibers of nonribosomal transcription unit as well as for most fiber-free chromatin. The detection of specific sites of transcriptional initiation and termination and the classification of transcription units can provide a basis for further analysis of transcriptional control.", "contents": "Comparative organization of active transcription units in Oncopeltus fasciatus. We have analyzed electron micrographs of chromatin-associated fiber arrays from embryos of the milkweed bug, Oncopeltus fasciatus. The analysis has revealed that the arrays have highly ordered patterns of fiber spacings and lengths. These patterns support the interpretation that the fibers are nascent RNA with associated proteins (RNP fibers) which have resulted from transcription of the DNA in the underlying chromatin segment. In particular, the patterns indicate that the chromatin underlying each array is delimited by specific sites for initiation and termination of transcription. We apply the term transcription unit to a chromatin segment thus bounded. The analysis has further revealed that transcription units can be grouped into two principal classes--ribosomal and nonribosomal. Active transcription units of these two classes differ in DNA content, in their proximity to other active transcription units, and in their chromatin morphology. For certain developmental stages, fiber frequencies (that is, the nubmers of fibers per mum of chromatin) are also useful in distinguishing ribosomal from nonribosomal arrays. The most definitive of the above classification criteria is chromatin morphology as observed under our preparative conditions. We propose that term rho chromatin for the unbeaded or smooth chromatin that underlies nascent ribosomal RNP fibers. DNA in rho chromatin has a calculated packing ratio of approximately 1.2 mum of B structure DNA per mum of chromatin. Nu chromatin is used to designate the beaded chromatin for which we calculate a DNA packing ratio of 1.6-2.3 in our preparations. This calculation for nu chromatin is based on the inference that the beads are nucleosomes (nu bodies, PS particles, unit particles). The beaded morphology is observed between fibers of nonribosomal transcription unit as well as for most fiber-free chromatin. The detection of specific sites of transcriptional initiation and termination and the classification of transcription units can provide a basis for further analysis of transcriptional control.", "PMID": 975237}
{"id": "pubmed23n0032_10237", "title": "Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration.", "content": "Flagellar regeneration in gametes of Chlamydomonas reinhardi is initiated within 15-20 min after flagellar amputation and proceeds at a rapid but decelerating rate until by 90 min flagellar outgrowth in 80-85% complete. Sufficient flagellar protein reserves exist in the cytoplasm to allow regeneration of flagella 1/3-1/2 normal length. Nevertheless, in vivo labeling with 14C-amino acids shows that microtubule protein and other flagellar proteins are synthesized de novo during flagellar regeneration. To determine whether tubulin is synthesized continuously by gametic cells or whether its synthesis is induced as a consequence of deflagellation, we have isolated polyribosomes from deflagellated and control cells, and analyzed the proteins produced by these polyribosomes during in vitro translation. Two proteins of 53,000 and 56,000 molecular weight which co-migrate with flagellar and chick brain tubulin on SDS-polyacrylamide gels and which selectively co-assemble with chick brain tubulin during in vitro microtubule assembly are synthesized by polyribosomes (or polyadenylated mRNA) from deflagellated cells. No microtubule proteins can be detected in the translation products synthesized by polyribosomes (or mRNA) from control cells, clearly indicating that deflagellation results in the induction ot tubulin synthesis. Kinetics of tubulin synthesis demonstrate that induction takes place immediately after deflagellation; polyribosomes bearing tubulin mRNA can be detected in the cytoplasm in as little as 15 min after removal of flagella. Maximal rates of tubulin synthesis occur between 45 and 90 min after deflagellation when approximately 14% of the protein being synthesized by the cell is tubulin. This estimate of tubulin synthesis based on in vitro translation data agrees well with in vivo measurements of flagellar tubulin synthesis. While high levels of tubulin production extend well beyond the period of rapid flagellar assembly, synthesis begins to decline after 90 min, and by 180 min after deflagellation only low levels of tubulin mRNA are detectable in polyribosomes.", "contents": "Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration. Flagellar regeneration in gametes of Chlamydomonas reinhardi is initiated within 15-20 min after flagellar amputation and proceeds at a rapid but decelerating rate until by 90 min flagellar outgrowth in 80-85% complete. Sufficient flagellar protein reserves exist in the cytoplasm to allow regeneration of flagella 1/3-1/2 normal length. Nevertheless, in vivo labeling with 14C-amino acids shows that microtubule protein and other flagellar proteins are synthesized de novo during flagellar regeneration. To determine whether tubulin is synthesized continuously by gametic cells or whether its synthesis is induced as a consequence of deflagellation, we have isolated polyribosomes from deflagellated and control cells, and analyzed the proteins produced by these polyribosomes during in vitro translation. Two proteins of 53,000 and 56,000 molecular weight which co-migrate with flagellar and chick brain tubulin on SDS-polyacrylamide gels and which selectively co-assemble with chick brain tubulin during in vitro microtubule assembly are synthesized by polyribosomes (or polyadenylated mRNA) from deflagellated cells. No microtubule proteins can be detected in the translation products synthesized by polyribosomes (or mRNA) from control cells, clearly indicating that deflagellation results in the induction ot tubulin synthesis. Kinetics of tubulin synthesis demonstrate that induction takes place immediately after deflagellation; polyribosomes bearing tubulin mRNA can be detected in the cytoplasm in as little as 15 min after removal of flagella. Maximal rates of tubulin synthesis occur between 45 and 90 min after deflagellation when approximately 14% of the protein being synthesized by the cell is tubulin. This estimate of tubulin synthesis based on in vitro translation data agrees well with in vivo measurements of flagellar tubulin synthesis. While high levels of tubulin production extend well beyond the period of rapid flagellar assembly, synthesis begins to decline after 90 min, and by 180 min after deflagellation only low levels of tubulin mRNA are detectable in polyribosomes.", "PMID": 975238}
{"id": "pubmed23n0032_10238", "title": "Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII.", "content": "The surface proteins of cultured human skin fibroblasts were iodinated and then exposed to one or more of the following blood coagulation proteins: thrombin, fibrinogen, and factor XIII (plasma protransglutaminase). Radiolabeled polypeptides were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. After exposure to physiological concentrations of activated factor XIII (XIIIa), the band of radioactivity corresponding to the major labeled surface protein (fibronectin, molecular weight = 2.2 x 10(5) daltons) was cross-linked to a very high molecular weight complex. The cross-linking reaction was inhibited by fibrin (which is known to bind the catalytic subunit of XIIIa). Cross-linking of labeled cell surface fibronectin to fibrin could not be demonstrated. The fibrillar pattern of surface fibronectin appeared unaffected by cross-linking when studied by immunofluorescence. Cross-linking of cell surface fibronectin by XIIIa requires highly specific enzyme-substrate and protein-protein interactions, and may be an important physiological reaction.", "contents": "Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII. The surface proteins of cultured human skin fibroblasts were iodinated and then exposed to one or more of the following blood coagulation proteins: thrombin, fibrinogen, and factor XIII (plasma protransglutaminase). Radiolabeled polypeptides were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. After exposure to physiological concentrations of activated factor XIII (XIIIa), the band of radioactivity corresponding to the major labeled surface protein (fibronectin, molecular weight = 2.2 x 10(5) daltons) was cross-linked to a very high molecular weight complex. The cross-linking reaction was inhibited by fibrin (which is known to bind the catalytic subunit of XIIIa). Cross-linking of labeled cell surface fibronectin to fibrin could not be demonstrated. The fibrillar pattern of surface fibronectin appeared unaffected by cross-linking when studied by immunofluorescence. Cross-linking of cell surface fibronectin by XIIIa requires highly specific enzyme-substrate and protein-protein interactions, and may be an important physiological reaction.", "PMID": 975239}
{"id": "pubmed23n0032_10239", "title": "Studies on the activity of the X chromosomes in female teratocarcinoma cells in culture.", "content": "Embryonal carcinoma cells derived from murine teratocarcinomas are able to differentiate into the same variety of tissue types as early embryonic cells. Because embryonal carcinoma cells resemble those of the embryo at a stage before X chromosome inactivation has occurred in females embryonal carcinoma cells containing two X chromosomes were examined to determine whether both are genetically active. The specific activities of X-linked enzymes were measured in embryonal carcinoma cells containing either one or two X chromosomes. The activities in both cell types were similar, suggesting that only one X chromosome was active in the female cells. Further support for this conclusion came from experiments in which azaguanine-resistant mutants were recovered with similar frequencies from embryonal carcinoma cell lines containing one and two X chromosomes. Late replication of an X chromosome DNA was detected in one embryonal carcinoma cell line with two X chromosomes but not in another. This suggests that cells of these two lines were arrested at different developmental stages, and that late DNA replication may not be a necessary adjunct of X inactivation. Evidence is presented which suggests that X chromosome reactivation does not occur during differentiation of the cells in vitro.", "contents": "Studies on the activity of the X chromosomes in female teratocarcinoma cells in culture. Embryonal carcinoma cells derived from murine teratocarcinomas are able to differentiate into the same variety of tissue types as early embryonic cells. Because embryonal carcinoma cells resemble those of the embryo at a stage before X chromosome inactivation has occurred in females embryonal carcinoma cells containing two X chromosomes were examined to determine whether both are genetically active. The specific activities of X-linked enzymes were measured in embryonal carcinoma cells containing either one or two X chromosomes. The activities in both cell types were similar, suggesting that only one X chromosome was active in the female cells. Further support for this conclusion came from experiments in which azaguanine-resistant mutants were recovered with similar frequencies from embryonal carcinoma cell lines containing one and two X chromosomes. Late replication of an X chromosome DNA was detected in one embryonal carcinoma cell line with two X chromosomes but not in another. This suggests that cells of these two lines were arrested at different developmental stages, and that late DNA replication may not be a necessary adjunct of X inactivation. Evidence is presented which suggests that X chromosome reactivation does not occur during differentiation of the cells in vitro.", "PMID": 975240}
{"id": "pubmed23n0032_10240", "title": "Delta-beta-thalassemia is due to a gene deletion.", "content": "DNA has been prepared from peripheral blood or cultured skin fibroblasts obtained from three Sicilian and one Greed deltabeta-thalassemia homozygotes. Globin-gene analysis was carried out using a cDNAbeta probe, and the results indicate that deltabeta-thalassemia has arisen from a deletion of the beta-globin genes. A similar result was obtained using DNA prepared from cultured skin fibroblasts from an individual homozygous for the Negro form of hereditary persistence of fetal hemoglobin (HPFH). In both cases, the deletion has spared the Ggamma and Agamma loci directing the gamma chains of hemoglobin F, but it has not been possible to demonstrate any difference between the size of the deletion involved in the production of delta-beta-thalassemia and that which gave rise to HPFH. These experiments provide further direct evidence that deletions of critical areas of the gamma-delta-beta gene cluster result in persistent gamma chain synthesis in adult life.", "contents": "Delta-beta-thalassemia is due to a gene deletion. DNA has been prepared from peripheral blood or cultured skin fibroblasts obtained from three Sicilian and one Greed deltabeta-thalassemia homozygotes. Globin-gene analysis was carried out using a cDNAbeta probe, and the results indicate that deltabeta-thalassemia has arisen from a deletion of the beta-globin genes. A similar result was obtained using DNA prepared from cultured skin fibroblasts from an individual homozygous for the Negro form of hereditary persistence of fetal hemoglobin (HPFH). In both cases, the deletion has spared the Ggamma and Agamma loci directing the gamma chains of hemoglobin F, but it has not been possible to demonstrate any difference between the size of the deletion involved in the production of delta-beta-thalassemia and that which gave rise to HPFH. These experiments provide further direct evidence that deletions of critical areas of the gamma-delta-beta gene cluster result in persistent gamma chain synthesis in adult life.", "PMID": 975241}
{"id": "pubmed23n0032_10241", "title": "An electron microscope study of the relative positions of the 4S and ribosomal RNA genes in HeLa cells mitochondrial DNA.", "content": "The 4S RNA genes in HeLa mitochondrial DNA (mtDNA) have been mapped by electron microscopy using the electron-opaque label ferritin. This method is based on the high affinity interaction between the protein, avidin,and biotin. 4S RNA, covalently coupled to biotin, was hybridized to single-stranded mtDNA. The hybrids were then labeled with ferritin-avidin conjugates. The positions of ferritin-labeled 4S RNA genes were determined relative to the rRNA genes on both heavy (H) and light (L) strands of mtDNA. This region was recognized as a duplex segment after hybridization either with rRNA in the case of H strands or with DNA complementary to rRNA in the case of L strands. Our studies suggest that at least nineteen 4S RNA genes are present in the HeLa mitochondrial genome. On the H strand, we have confirmed the nine map positions found in a previous electron microscope mapping study (Wu et al., 1972) and obtained evidence for three additional 4S RNA genes. On the L strand, seven 4S RNA genes have been mapped. The nineteen genes are distributed more or less uniformly around the genome. There is a pair of closely spaced genes, approximately 150 nucleotides apart, on the H strand, and another closely spaced pair on the L strand.", "contents": "An electron microscope study of the relative positions of the 4S and ribosomal RNA genes in HeLa cells mitochondrial DNA. The 4S RNA genes in HeLa mitochondrial DNA (mtDNA) have been mapped by electron microscopy using the electron-opaque label ferritin. This method is based on the high affinity interaction between the protein, avidin,and biotin. 4S RNA, covalently coupled to biotin, was hybridized to single-stranded mtDNA. The hybrids were then labeled with ferritin-avidin conjugates. The positions of ferritin-labeled 4S RNA genes were determined relative to the rRNA genes on both heavy (H) and light (L) strands of mtDNA. This region was recognized as a duplex segment after hybridization either with rRNA in the case of H strands or with DNA complementary to rRNA in the case of L strands. Our studies suggest that at least nineteen 4S RNA genes are present in the HeLa mitochondrial genome. On the H strand, we have confirmed the nine map positions found in a previous electron microscope mapping study (Wu et al., 1972) and obtained evidence for three additional 4S RNA genes. On the L strand, seven 4S RNA genes have been mapped. The nineteen genes are distributed more or less uniformly around the genome. There is a pair of closely spaced genes, approximately 150 nucleotides apart, on the H strand, and another closely spaced pair on the L strand.", "PMID": 975242}
{"id": "pubmed23n0032_10242", "title": "The isolation and preliminary characterization of somatic cell mutants resistant to the protein synthesis inhibitor-emetine.", "content": "Emetine reversibly inhibits protein synthesis in Chinese hamster ovary (CHO) cells. Stable mutants which are 20-80 fold more resistant to the cytotoxic action of the drug can be isolated in a  single step at a frequency of about 2-5 X 10(-7). The frequency of such mutants is increased 30-50 fold by ethyl methane sulphonate mutagenesis, and the spontaneous rate of mutation to emetine resistance as measured by Luria-Delbruck fluctuation analyses is 4.9 X 10(-7) mutations per locus per generation. Protein synthesis in extracts of the mutant cells is resistant to the inhibitory action of the emetine, indicating that the molecular lesion in these cells lies in the protein synthesis machinery.", "contents": "The isolation and preliminary characterization of somatic cell mutants resistant to the protein synthesis inhibitor-emetine. Emetine reversibly inhibits protein synthesis in Chinese hamster ovary (CHO) cells. Stable mutants which are 20-80 fold more resistant to the cytotoxic action of the drug can be isolated in a  single step at a frequency of about 2-5 X 10(-7). The frequency of such mutants is increased 30-50 fold by ethyl methane sulphonate mutagenesis, and the spontaneous rate of mutation to emetine resistance as measured by Luria-Delbruck fluctuation analyses is 4.9 X 10(-7) mutations per locus per generation. Protein synthesis in extracts of the mutant cells is resistant to the inhibitory action of the emetine, indicating that the molecular lesion in these cells lies in the protein synthesis machinery.", "PMID": 975243}
{"id": "pubmed23n0032_10243", "title": "Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis.", "content": "A method for the clonal analysis of murine erythroleukemia cells has been developed which allows the precise characterization of the number of progeny produced by each cell and the degree of differentiation of each progeny cell. The potential of almost every cell in the culture can be monitored because a plating efficiency close to 100% has been achieved. The effects of treatment with an inducer of differentiation (DMSO) on the proliferative capacity of the treated cells have been studied with this technique. Cells from a mass culture treated with inducer give rise to colonies of differentiated progeny when subsequently cloned in the absence of inducer. Colonies exhibiting this phenotype represent the progeny of cells committed to the differentiation pathway by treatment with inducer. We observe that the commitment decision limits the subsequent proliferative capacity of the cell to four additional cell divisions. A quantitative analysis suggests that the commitment decision for each cell is made in a stochastic manner. Irreversible commitment to the expression of differentiated functions occurs with discrete probability per cell generation for many cell generations. The value for this probability is a function of the concentration of inducer (DMSO). A correlative biochemical study suggests that an irreversible commitment decision by a significant proportion of the population precedes or accompanies increases in cytoplasmic globin mRNA levels, one of the earliest detectable biochemical markers for erythroid differentiation in this system. A specific kinetic model based on these considerations has been developed to predict clonal phenotypes as a function of time and probability of commitment. Quantitative predictions based on this model are in excellent agreement with experimental observations. The effectiveness of a stochastic model in predicting the behavior of this system is discussed in relation to the stochastic behavior of normal hematopoiesis and the biochemical mechanisms which control these differentiation programs.", "contents": "Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. A method for the clonal analysis of murine erythroleukemia cells has been developed which allows the precise characterization of the number of progeny produced by each cell and the degree of differentiation of each progeny cell. The potential of almost every cell in the culture can be monitored because a plating efficiency close to 100% has been achieved. The effects of treatment with an inducer of differentiation (DMSO) on the proliferative capacity of the treated cells have been studied with this technique. Cells from a mass culture treated with inducer give rise to colonies of differentiated progeny when subsequently cloned in the absence of inducer. Colonies exhibiting this phenotype represent the progeny of cells committed to the differentiation pathway by treatment with inducer. We observe that the commitment decision limits the subsequent proliferative capacity of the cell to four additional cell divisions. A quantitative analysis suggests that the commitment decision for each cell is made in a stochastic manner. Irreversible commitment to the expression of differentiated functions occurs with discrete probability per cell generation for many cell generations. The value for this probability is a function of the concentration of inducer (DMSO). A correlative biochemical study suggests that an irreversible commitment decision by a significant proportion of the population precedes or accompanies increases in cytoplasmic globin mRNA levels, one of the earliest detectable biochemical markers for erythroid differentiation in this system. A specific kinetic model based on these considerations has been developed to predict clonal phenotypes as a function of time and probability of commitment. Quantitative predictions based on this model are in excellent agreement with experimental observations. The effectiveness of a stochastic model in predicting the behavior of this system is discussed in relation to the stochastic behavior of normal hematopoiesis and the biochemical mechanisms which control these differentiation programs.", "PMID": 975244}
{"id": "pubmed23n0032_10244", "title": "Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm.", "content": "We have surveyed the early stages in the development and differentiation of cultured mouse embryos for plasminogen activator production. This enzyme is first detectable by the sixth equivalent gestation day. Thereafter, cultured blastocysts produce plasminogen activator with a biphasic time course: in the first phase, enzyme secretion rises to a maximum at about the eighth day and then decreases; a second phase, during which more enzyme accumulates, begins somewhat later and continues to at least the fifteenth day. By fractionating the blastocyst into its constituent cell types, we have identified the trophoblast as the cells responsible for the first phase of enzyme synthesis. The pattern of enzyme production by the trophoblast is closely correlated with the invasive period of these cells in vivo and implies that plasminogen activator is involved in embryo implantation. The second phase of plasminogen activator production is due to parietal endoderm, which initiates enzyme synthesis upon differentiation from the inner cell mass. The properties of the parietal endoderm suggest that plasminogen activator may participate in the migration of these cells and/or in the metabolism of Reichert's membrane which accompanies embryo growth. These results are consistent with the concept, deveolped from work on other cell types, that plasminogen activator may represent a generalized mechanism for tissue remodeling and cell migration.", "contents": "Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm. We have surveyed the early stages in the development and differentiation of cultured mouse embryos for plasminogen activator production. This enzyme is first detectable by the sixth equivalent gestation day. Thereafter, cultured blastocysts produce plasminogen activator with a biphasic time course: in the first phase, enzyme secretion rises to a maximum at about the eighth day and then decreases; a second phase, during which more enzyme accumulates, begins somewhat later and continues to at least the fifteenth day. By fractionating the blastocyst into its constituent cell types, we have identified the trophoblast as the cells responsible for the first phase of enzyme synthesis. The pattern of enzyme production by the trophoblast is closely correlated with the invasive period of these cells in vivo and implies that plasminogen activator is involved in embryo implantation. The second phase of plasminogen activator production is due to parietal endoderm, which initiates enzyme synthesis upon differentiation from the inner cell mass. The properties of the parietal endoderm suggest that plasminogen activator may participate in the migration of these cells and/or in the metabolism of Reichert's membrane which accompanies embryo growth. These results are consistent with the concept, deveolped from work on other cell types, that plasminogen activator may represent a generalized mechanism for tissue remodeling and cell migration.", "PMID": 975245}
{"id": "pubmed23n0032_10245", "title": "Two complementary strand-specific termination sites for adenovirus DNA replication.", "content": "Adenovirus type 2 DNA, specifically labeled at the termini for DNA replication, was prepared by isolation of viral DNA molecules which were completed during short pulses with 3H-thymidine. The distribution of radioactivity in the two complementary strands at the termini for DNA replication was determined by liquid phase hybridization and gelelectrophoresis. At the right-hand terminus, nearly all radioactivity was found in the viral h strand, whereas at the left-hand terminus, most radioactivity was confined to the viral l strand. The results suggest that both molecular ends serve as origins and termini for replication of adenovirus type 2 DNA.", "contents": "Two complementary strand-specific termination sites for adenovirus DNA replication. Adenovirus type 2 DNA, specifically labeled at the termini for DNA replication, was prepared by isolation of viral DNA molecules which were completed during short pulses with 3H-thymidine. The distribution of radioactivity in the two complementary strands at the termini for DNA replication was determined by liquid phase hybridization and gelelectrophoresis. At the right-hand terminus, nearly all radioactivity was found in the viral h strand, whereas at the left-hand terminus, most radioactivity was confined to the viral l strand. The results suggest that both molecular ends serve as origins and termini for replication of adenovirus type 2 DNA.", "PMID": 975246}
{"id": "pubmed23n0032_10246", "title": "Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.", "content": "We have used lambda-32P-ATP and polynucleotide kinase to label stoichiometrically the 5' ends of DNA in intact isolated HeLa cell nucleosomes. DNA is not nicked or degraded during the modification reaction. We have used these modified nucleosomes to study both the distribution and the relative availability of sites within the nucleosome which are susceptible to digestion by DNAase l. The results show that the nucleosome contains a potential cleavage site every 10 nucleotides, with the exception of the site 80 nucleotides from the 5' end. Favored cleavage sites are located 20, 40, 50, 100, 120, and 130 nucleotides from the 5' end; sites 30 and 110 nucleotides from the 5' end are strongly disfavored, while the potential site 80 nucleotides from the 5' end is virtually never cleaved. These findings provide constraints for models of histone-DNA interactions within the chromatin subunit.", "contents": "Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends. We have used lambda-32P-ATP and polynucleotide kinase to label stoichiometrically the 5' ends of DNA in intact isolated HeLa cell nucleosomes. DNA is not nicked or degraded during the modification reaction. We have used these modified nucleosomes to study both the distribution and the relative availability of sites within the nucleosome which are susceptible to digestion by DNAase l. The results show that the nucleosome contains a potential cleavage site every 10 nucleotides, with the exception of the site 80 nucleotides from the 5' end. Favored cleavage sites are located 20, 40, 50, 100, 120, and 130 nucleotides from the 5' end; sites 30 and 110 nucleotides from the 5' end are strongly disfavored, while the potential site 80 nucleotides from the 5' end is virtually never cleaved. These findings provide constraints for models of histone-DNA interactions within the chromatin subunit.", "PMID": 975247}
{"id": "pubmed23n0032_10247", "title": "Influence of lead nitrate on dimethylnitrosamine intoxication.", "content": "The effect of lead nitrate, an inhibitor of the hepatic drug-metabolizing enzyme system upon the acute, hepatotoxicity of dimethylnitrosamine (DMN) was studied. Lead pretreatment significantly prevented polysomal disaggregation induced by the nitrosamine. Cell necrosis, evaluated morphologically and by the release of serum glutamic-pyruvic transaminase (GPT), was also diminished. The metabolism of DMN in rats pretreated with lead nitrate was investigated by following its clearance from blood and by determining, in vitro the demethylation of the nitrosamine. Lead increased, although not significantly, the clearance of DMN from blood, but it lowered the activity of DMN-demethylase 24 h after its administration. Finally, lead lowered the lethal effects of DMN. The mechanism by which lead influenced DMN toxicity is discussed.", "contents": "Influence of lead nitrate on dimethylnitrosamine intoxication. The effect of lead nitrate, an inhibitor of the hepatic drug-metabolizing enzyme system upon the acute, hepatotoxicity of dimethylnitrosamine (DMN) was studied. Lead pretreatment significantly prevented polysomal disaggregation induced by the nitrosamine. Cell necrosis, evaluated morphologically and by the release of serum glutamic-pyruvic transaminase (GPT), was also diminished. The metabolism of DMN in rats pretreated with lead nitrate was investigated by following its clearance from blood and by determining, in vitro the demethylation of the nitrosamine. Lead increased, although not significantly, the clearance of DMN from blood, but it lowered the activity of DMN-demethylase 24 h after its administration. Finally, lead lowered the lethal effects of DMN. The mechanism by which lead influenced DMN toxicity is discussed.", "PMID": 975397}
{"id": "pubmed23n0032_10248", "title": "Effects of N-methyl-N-nitrosourethane on DNA synthesis in the guinea pig pancreas.", "content": "The effects of N-methyl-N-nitrosourethane (NMUT) on pancreatic DNA synthesis were investigated at sequential intervals following gavage of Hartley guinea pigs with a single dose of 30 mg/kg. There was a highly significant stimulation of DNA synthesis, as evidenced by increased incorporation of [3H] methyl-thymidine ([3H]TdR), throughout the whole pancreas and particularly in the duodenal segment, at 4 h following NMUT administration, thereafter, DNA synthesis declined sharply up to 24 h, and then recovered gradually to control levels from 24--96 h. DNA synthesis stimulated by NMUT was suppressed by hydroxyurea (HU), and hence is likely to represent replicative, rather than repair, synthesis.", "contents": "Effects of N-methyl-N-nitrosourethane on DNA synthesis in the guinea pig pancreas. The effects of N-methyl-N-nitrosourethane (NMUT) on pancreatic DNA synthesis were investigated at sequential intervals following gavage of Hartley guinea pigs with a single dose of 30 mg/kg. There was a highly significant stimulation of DNA synthesis, as evidenced by increased incorporation of [3H] methyl-thymidine ([3H]TdR), throughout the whole pancreas and particularly in the duodenal segment, at 4 h following NMUT administration, thereafter, DNA synthesis declined sharply up to 24 h, and then recovered gradually to control levels from 24--96 h. DNA synthesis stimulated by NMUT was suppressed by hydroxyurea (HU), and hence is likely to represent replicative, rather than repair, synthesis.", "PMID": 975398}
{"id": "pubmed23n0032_10249", "title": "Identification of the persistently bound form of the carcinogen N-acetyl-2-aminofluorene to rat liver DNA in vivo.", "content": "In this article the structural analysis of the persistently bound form of the carcinogen N-acetyl-2-aminofluorene (AAF) to rat liver DNA in vivo is described. This compound appears to result from the formation of a covalent bond between carbon-3 of the aromatic ring and the amino group of guanine. Experimental evidence from three different approaches had led to the identification of the structure of the persistently DNA-bound AAF moiety. First, [3-3H, 9-14C]N-acetoxy-AAF was reacted with DNA in vitro. As reported previously, a minor product was isolated from enzymatic digests of the reacted DNA, which had chemical and chromatographic properties identical to those of the persistent--AAF moiety in DNA in vivo. The ratio 3H/14C of this product had diminished to the same extent as 3-CH3S-AAF resulting from the reaction of methionine with [o-3H, 9-14C]N-acetoxy-AAF. Secondly, reaction of [9-14C]N-acetoxy-AAF with DNA, which was tritiated in the C-8 positions of the purines, did not result in removal of tritium in the persistent fraction obtained after acid hydrolysis, thus excluding substitution at C-8 and N-7 of guanine. Finally , by reacting N-OSO3-K-AAF with deoxyguanosine in dimethylsulfoxide-triethylamine, a compound could be isolated, which was identified as 3-(deoxyguanosin-N2-yl)-AAF based on its NMR spectrum and on the mass spectrum of the corresponding guanine derivative obtained after removing deoxyribose by acid hydrolysis. This compound appeared to be identical with the persistently bound form present in DNA hydrolysates from rat liver after injection of [2'-3H]N-hydroxy-AAF.", "contents": "Identification of the persistently bound form of the carcinogen N-acetyl-2-aminofluorene to rat liver DNA in vivo. In this article the structural analysis of the persistently bound form of the carcinogen N-acetyl-2-aminofluorene (AAF) to rat liver DNA in vivo is described. This compound appears to result from the formation of a covalent bond between carbon-3 of the aromatic ring and the amino group of guanine. Experimental evidence from three different approaches had led to the identification of the structure of the persistently DNA-bound AAF moiety. First, [3-3H, 9-14C]N-acetoxy-AAF was reacted with DNA in vitro. As reported previously, a minor product was isolated from enzymatic digests of the reacted DNA, which had chemical and chromatographic properties identical to those of the persistent--AAF moiety in DNA in vivo. The ratio 3H/14C of this product had diminished to the same extent as 3-CH3S-AAF resulting from the reaction of methionine with [o-3H, 9-14C]N-acetoxy-AAF. Secondly, reaction of [9-14C]N-acetoxy-AAF with DNA, which was tritiated in the C-8 positions of the purines, did not result in removal of tritium in the persistent fraction obtained after acid hydrolysis, thus excluding substitution at C-8 and N-7 of guanine. Finally , by reacting N-OSO3-K-AAF with deoxyguanosine in dimethylsulfoxide-triethylamine, a compound could be isolated, which was identified as 3-(deoxyguanosin-N2-yl)-AAF based on its NMR spectrum and on the mass spectrum of the corresponding guanine derivative obtained after removing deoxyribose by acid hydrolysis. This compound appeared to be identical with the persistently bound form present in DNA hydrolysates from rat liver after injection of [2'-3H]N-hydroxy-AAF.", "PMID": 975399}
{"id": "pubmed23n0032_10250", "title": "Involvement of polyphosphorylserine blocks in the Fe(III) binding by phosvitin.", "content": "It has been suggested that the binding of iron(III) by phosvitin involves the phosphoric radicals of phosphorylserine residues, many of which are arranged in rows of several consecutive phosphoamino acids. In this paper we present evident that, unlike free phosphorylserine which does not interact with Fe(III), polyphosphorylserine blocks--(Ser-P)n, with n greater than or equal to 4 -- bind Fe(III) like phosvitin, though less actively, to give complexes stable at very acidic pHs. The binding  of iron does not cause any polymerization of the phosphopeptides, and the (Ser-P)n-Fe(III) complexes display an Fe/P ratio significantly lower than 0.5, found in Fe-saturated phosvitin. These findings indicate that polyphosphorylserine blocks play an important role in the binding of iron by phosvitin, and that in the intact protein their binding capacity is optimized by the conformation of the polypeptide chain.", "contents": "Involvement of polyphosphorylserine blocks in the Fe(III) binding by phosvitin. It has been suggested that the binding of iron(III) by phosvitin involves the phosphoric radicals of phosphorylserine residues, many of which are arranged in rows of several consecutive phosphoamino acids. In this paper we present evident that, unlike free phosphorylserine which does not interact with Fe(III), polyphosphorylserine blocks--(Ser-P)n, with n greater than or equal to 4 -- bind Fe(III) like phosvitin, though less actively, to give complexes stable at very acidic pHs. The binding  of iron does not cause any polymerization of the phosphopeptides, and the (Ser-P)n-Fe(III) complexes display an Fe/P ratio significantly lower than 0.5, found in Fe-saturated phosvitin. These findings indicate that polyphosphorylserine blocks play an important role in the binding of iron by phosvitin, and that in the intact protein their binding capacity is optimized by the conformation of the polypeptide chain.", "PMID": 975400}
{"id": "pubmed23n0032_10251", "title": "The distribution of [3H]synthanecine A bis-N-ethylcarbamate and its metabolites in the rat.", "content": "An intravenous dose of 40 mg/kg [3H] synthanecine A bis-N-ethylcarbamate, sufficient to cause liver lesions similar to pyrrolizidine alkaloid poisoning, was given to male rats. The distribution of radioactivity in body tissues was measured after various times from 2 h to 340 days. About 69% of the dose was eliminated via urine and faeces on the first day, while less than 0.4% was found in expired air. The highest tissue level of radioactivity was initially in liver, but this fell steadily at a rate comparable with most other tissues. High levels were also found in kidneys, spleen and lungs, and elimination from the latter was exceptionally slow. A high level in the erythrocytes probably accounted for a persistence of radioactivity in the spleen. Ehrlich estimations showed that 68% of the radioactivity in the liver 2--6 h after dosing was present as pyrrolic metabolites. A high proportion of the liver activity was not extractable by alcohol or aqueous trichloroacetic acid. Binding in the microsomal and nuclear fractions of liver was surprisingly low. From the low level of radioactivity in stomach tissue it was concluded that 2,3-bishydroxymethyl-1-methyl pyrrole could not have been a major metabolite in the bloodstream, since the latter, when given intravenously, binds strongly to the glandular stomach.", "contents": "The distribution of [3H]synthanecine A bis-N-ethylcarbamate and its metabolites in the rat. An intravenous dose of 40 mg/kg [3H] synthanecine A bis-N-ethylcarbamate, sufficient to cause liver lesions similar to pyrrolizidine alkaloid poisoning, was given to male rats. The distribution of radioactivity in body tissues was measured after various times from 2 h to 340 days. About 69% of the dose was eliminated via urine and faeces on the first day, while less than 0.4% was found in expired air. The highest tissue level of radioactivity was initially in liver, but this fell steadily at a rate comparable with most other tissues. High levels were also found in kidneys, spleen and lungs, and elimination from the latter was exceptionally slow. A high level in the erythrocytes probably accounted for a persistence of radioactivity in the spleen. Ehrlich estimations showed that 68% of the radioactivity in the liver 2--6 h after dosing was present as pyrrolic metabolites. A high proportion of the liver activity was not extractable by alcohol or aqueous trichloroacetic acid. Binding in the microsomal and nuclear fractions of liver was surprisingly low. From the low level of radioactivity in stomach tissue it was concluded that 2,3-bishydroxymethyl-1-methyl pyrrole could not have been a major metabolite in the bloodstream, since the latter, when given intravenously, binds strongly to the glandular stomach.", "PMID": 975401}
{"id": "pubmed23n0032_10252", "title": "The distribution of radio-activity in rats given [3H]bishydroxymethyl-1-methylpyrrole and its relationship to that from [3H]synthanecine A bis-N-ethylcarbamate.", "content": "The distribution of radioactivity was measured in rats various times after single intravenous doses of tritium-labelled 2,3-bis-hydroxymethyl-1-methylpyrrole (BHMP). Over half the dose was excreted in urine during the first day; less than a seventh this amount was found in the faeces. The level in glandular stomach was much higher than in any other organ and there was evidence that this was related to the acidity of this tissue. With this exception, the radioactivity in other tissues was lower than after an equivalent dose of tritiated synthanecine A bis-N-ethylcarbamate, and that in liver tissue was more easily solubilised than after the latter compound. The results indicate it is unlikely that more than a small amount of free BHMP is released into the bloodstreams of rats given synthanecine A bis-N-ethylcarbamate. When [3H]BHMP is given by stomach tube much radioactivity remains within the gut and there is no exceptional binding to glandular stomach tissue. Whereas the tissue binding as well as chemical and toxicological properties of BHMP are similar to those of dehydroretronecine, the binding properties of synthanecine A bis-N-ethylcarbamate are likely to resemble those of monocrotaline and similar pyrrolizidine alkaloids.", "contents": "The distribution of radio-activity in rats given [3H]bishydroxymethyl-1-methylpyrrole and its relationship to that from [3H]synthanecine A bis-N-ethylcarbamate. The distribution of radioactivity was measured in rats various times after single intravenous doses of tritium-labelled 2,3-bis-hydroxymethyl-1-methylpyrrole (BHMP). Over half the dose was excreted in urine during the first day; less than a seventh this amount was found in the faeces. The level in glandular stomach was much higher than in any other organ and there was evidence that this was related to the acidity of this tissue. With this exception, the radioactivity in other tissues was lower than after an equivalent dose of tritiated synthanecine A bis-N-ethylcarbamate, and that in liver tissue was more easily solubilised than after the latter compound. The results indicate it is unlikely that more than a small amount of free BHMP is released into the bloodstreams of rats given synthanecine A bis-N-ethylcarbamate. When [3H]BHMP is given by stomach tube much radioactivity remains within the gut and there is no exceptional binding to glandular stomach tissue. Whereas the tissue binding as well as chemical and toxicological properties of BHMP are similar to those of dehydroretronecine, the binding properties of synthanecine A bis-N-ethylcarbamate are likely to resemble those of monocrotaline and similar pyrrolizidine alkaloids.", "PMID": 975402}
{"id": "pubmed23n0032_10253", "title": "Fatty acids. Part 48. 13C nuclear magnetic resonance studies of acetylenic fatty acids.", "content": "The 13C NMR spectra of thirty-seven alkynoic acids (C8-C18) and ten alkadiynoic acids (C18 and C20) are reported and interpreted. The influence of COOH, COOCH3, CH3, and C identical to C groups on the chemical shifts of nearby carbon atoms is assessed. These influences are largely additive so that available spectra are readily interpreted and the spectra of new compounds of this type can be predicted. These preliminary results indicate that 13C NMR spectroscopy should be of considerable value in the structural identification of acetylenic compounds.", "contents": "Fatty acids. Part 48. 13C nuclear magnetic resonance studies of acetylenic fatty acids. The 13C NMR spectra of thirty-seven alkynoic acids (C8-C18) and ten alkadiynoic acids (C18 and C20) are reported and interpreted. The influence of COOH, COOCH3, CH3, and C identical to C groups on the chemical shifts of nearby carbon atoms is assessed. These influences are largely additive so that available spectra are readily interpreted and the spectra of new compounds of this type can be predicted. These preliminary results indicate that 13C NMR spectroscopy should be of considerable value in the structural identification of acetylenic compounds.", "PMID": 975444}
{"id": "pubmed23n0032_10254", "title": "Fatty acids and long-chain bases of gangliosides of human gastrointestinal mucosa.", "content": "The fatty acid and long-chain base composition of five major gangliosides from human stomach and small and large intestine mucosa were analyzed with gas chromatography. All the gangliosides greatly resembled each other in the fatty acid pattern. The main fatty acids were C16:0, C18:0, C22:0 and C24:1. No hydroxy fatty acids could be detected. In all the gangliosides 4-sphingenine was the predominant long-chain base (70-75%). About 15% of the long-chain bases had 20 carbon atoms in their chain. No trihydroxy long-chain bases could be detected.", "contents": "Fatty acids and long-chain bases of gangliosides of human gastrointestinal mucosa. The fatty acid and long-chain base composition of five major gangliosides from human stomach and small and large intestine mucosa were analyzed with gas chromatography. All the gangliosides greatly resembled each other in the fatty acid pattern. The main fatty acids were C16:0, C18:0, C22:0 and C24:1. No hydroxy fatty acids could be detected. In all the gangliosides 4-sphingenine was the predominant long-chain base (70-75%). About 15% of the long-chain bases had 20 carbon atoms in their chain. No trihydroxy long-chain bases could be detected.", "PMID": 975445}
{"id": "pubmed23n0032_10255", "title": "Solubility of phosphatidylcholine in chloroform. Formation of hydrogen bonding between phosphatidylcholine and chloroform.", "content": "The solubility of phosphatidylcholine (PC) was studied by the spectroscopic analysis and the measurement of the solubility. The qualitative analysis of infrared absorption spectra confirmed the existence of two types of hydrogen bondings between chloroform and PC, one between chloroform and the C=O group of PC and the other between chloroform and the phosphorylcholine group of PC. The quantitative analysis of the C-D stretching vibration bands of the chloroform-d solution of PC showed that the latter hydrogen bonding mainly contributes to the solubility and that PC dissolves in chloroform to form a complex consisting of a few or more molecules of chloroform and one molecule of PC. We discussed in this report about the molecular organization of PC in chloroform solution.", "contents": "Solubility of phosphatidylcholine in chloroform. Formation of hydrogen bonding between phosphatidylcholine and chloroform. The solubility of phosphatidylcholine (PC) was studied by the spectroscopic analysis and the measurement of the solubility. The qualitative analysis of infrared absorption spectra confirmed the existence of two types of hydrogen bondings between chloroform and PC, one between chloroform and the C=O group of PC and the other between chloroform and the phosphorylcholine group of PC. The quantitative analysis of the C-D stretching vibration bands of the chloroform-d solution of PC showed that the latter hydrogen bonding mainly contributes to the solubility and that PC dissolves in chloroform to form a complex consisting of a few or more molecules of chloroform and one molecule of PC. We discussed in this report about the molecular organization of PC in chloroform solution.", "PMID": 975447}
{"id": "pubmed23n0032_10256", "title": "Phase transition in a lipid bilayer. II. Influence of adamantane derivatives.", "content": "The influence of thirty-four adamantane, protoadamantane, and homoadamantane derivatives on the phase transition characteristics of the bilayer in dipalmitoyl lecithin liposomes has been determined by differential scanning calorimetry. Each of these compounds induces a broadening of the phase transition profile of the lipid bilayer that is dependent upon the concentration of the solute and its molecular structure. The concentration--response curves obtained for these solutes suggest that the cage compound derivatives modify the phase properties and under some conditions may induce a phase separation in the doped bilayer. The relative activity sequences obtained for the compounds examined cannot be accounted for by simple considerations of lipid/water partition coefficients, substitution constants based on free energy relationships, or the relative polarities or sizes of substituent groups. The observations are consistent with the hypothesis that the position and orientation of a solute within the bilayer are critical factors in determining its relative potency. The position of a solute within the bilayer is significantly controlled by the presence of polar substituents and by the relative geometric relationships of these groups. For a given substituent group, the shape and size of the hydrocarbon cage becomes increasingly important. It is apparent that seemingly minor modifications in the structure of a solute can significantly alter its influence on the phase transition behavior of a bilayer.", "contents": "Phase transition in a lipid bilayer. II. Influence of adamantane derivatives. The influence of thirty-four adamantane, protoadamantane, and homoadamantane derivatives on the phase transition characteristics of the bilayer in dipalmitoyl lecithin liposomes has been determined by differential scanning calorimetry. Each of these compounds induces a broadening of the phase transition profile of the lipid bilayer that is dependent upon the concentration of the solute and its molecular structure. The concentration--response curves obtained for these solutes suggest that the cage compound derivatives modify the phase properties and under some conditions may induce a phase separation in the doped bilayer. The relative activity sequences obtained for the compounds examined cannot be accounted for by simple considerations of lipid/water partition coefficients, substitution constants based on free energy relationships, or the relative polarities or sizes of substituent groups. The observations are consistent with the hypothesis that the position and orientation of a solute within the bilayer are critical factors in determining its relative potency. The position of a solute within the bilayer is significantly controlled by the presence of polar substituents and by the relative geometric relationships of these groups. For a given substituent group, the shape and size of the hydrocarbon cage becomes increasingly important. It is apparent that seemingly minor modifications in the structure of a solute can significantly alter its influence on the phase transition behavior of a bilayer.", "PMID": 975448}
{"id": "pubmed23n0032_10257", "title": "Changes in cardiac output and total peripheral resistance during development of renal hypertension in the rabbit: lack of confomity with the autoregulation theory.", "content": "Serial measurements of cardiac output (CO), mean arterial pressure (MAP), heart rate, and total peripheral resistance (TPR), were made on unanesthetized rabbits with previously implanted Doppler flowmeters. After 2 days of control measurements the rabbits were subjected alternatively to bilateral renal cellophane wrapping (wrap group) or to sham operation and additional measurements were made 1, 2, 4, 8, 16, 25, and 32 days after operation. During the 1st week after operation changes in CO were identical in the wrap and sham-operated groups, with an overall increase to a value of 110% of control on day 4 (P less than 0.05). Thereafter CO fell gradually, reaching 75% of control by day 32 in the wrap group, but only 95% of control in the sham-operated group. When CO was expressed per unit of body weight the latter differences were somewhat reduced, but still were significant. In wrap animals MAP and TPR rose progressively to 155% and 194% of control by day 32. In the sham-operated group the corresponding increases to 108% and 118% of control were significantly smaller. The MAP and TPR of the renal wrap rabbits exceeded the values in sham-operated rabbits, even over the 1st week after operation, by an average for MAP of 8.6 +/- 1.4% (P less than 0.001), and for TPR of 8.0 +/- 2.5% (P = 0.01). The results suggest that the changes in CO during the 1st week were a nonspecific consequence of the preceding wrap or sham operation. They bore no apparent relationship to the subsequent development of the hypertension which was \"resistance-mediated\" from the earliest stages. We conclude that the present findings for the rabbit differ from those reported for other species and do not conform to the changes predicted by the autoregulation theory of the pathogenesis of hypertension.", "contents": "Changes in cardiac output and total peripheral resistance during development of renal hypertension in the rabbit: lack of confomity with the autoregulation theory. Serial measurements of cardiac output (CO), mean arterial pressure (MAP), heart rate, and total peripheral resistance (TPR), were made on unanesthetized rabbits with previously implanted Doppler flowmeters. After 2 days of control measurements the rabbits were subjected alternatively to bilateral renal cellophane wrapping (wrap group) or to sham operation and additional measurements were made 1, 2, 4, 8, 16, 25, and 32 days after operation. During the 1st week after operation changes in CO were identical in the wrap and sham-operated groups, with an overall increase to a value of 110% of control on day 4 (P less than 0.05). Thereafter CO fell gradually, reaching 75% of control by day 32 in the wrap group, but only 95% of control in the sham-operated group. When CO was expressed per unit of body weight the latter differences were somewhat reduced, but still were significant. In wrap animals MAP and TPR rose progressively to 155% and 194% of control by day 32. In the sham-operated group the corresponding increases to 108% and 118% of control were significantly smaller. The MAP and TPR of the renal wrap rabbits exceeded the values in sham-operated rabbits, even over the 1st week after operation, by an average for MAP of 8.6 +/- 1.4% (P less than 0.001), and for TPR of 8.0 +/- 2.5% (P = 0.01). The results suggest that the changes in CO during the 1st week were a nonspecific consequence of the preceding wrap or sham operation. They bore no apparent relationship to the subsequent development of the hypertension which was \"resistance-mediated\" from the earliest stages. We conclude that the present findings for the rabbit differ from those reported for other species and do not conform to the changes predicted by the autoregulation theory of the pathogenesis of hypertension.", "PMID": 975451}
{"id": "pubmed23n0032_10258", "title": "Localization of glucocorticoid uptake in normal and ischemic myocardial tissue of isolated perfused cat hearts.", "content": "We studied the uptake of labeled dexamethasone (3H-Dex) or methylprednisolone (3H-MP) in isolated perfused cat hearts during the first hour of acute myocardial ischemia. Considerable amounts of 3H-Dex and 3H-MP were taken up by the plasma membrane (F1) fraction in control, border zone, and ischemic myocardial tissue. Lesser amounts were incorporated into the remaining cell fractions. A gradient of glucocorticoid uptake was observed that decreased from control tissue to ischemic tissue in all subcellular fractions (i.e., F1 to F5). Accordingly, supernatant fraction (S) to particulate (P) ratios of labeled glucocorticoid uptake increased from control to ischemic tissue, indicating that myocardial cell damage resulted in a decrease in glucocorticoid-binding capacity in subcellular fractions obtained from ischemic tissue. The activity of 5'-nucleotidase (5'ND), a plasma membrane marker in myocardial cells, also decreased from normal to ischemic tissue. Furthermore, we found that uptake of 3H-MP and 3H-Dex was associated with the retention of 5'ND activity in F1 fractions of both border zone and ischemic tissue. Similar protection of plasma membrane integritg occurred in the supernatant fraction as determined by changes in S/P ratios of 5'ND activity. These data provide support for the concepts that (1) plasma membrane changes occur soon after acute myocardial ischemia, and (2) the mechanism by which glucocorticoids exert a protective effect in myocardial ischemia may be related to membrane stabilization.", "contents": "Localization of glucocorticoid uptake in normal and ischemic myocardial tissue of isolated perfused cat hearts. We studied the uptake of labeled dexamethasone (3H-Dex) or methylprednisolone (3H-MP) in isolated perfused cat hearts during the first hour of acute myocardial ischemia. Considerable amounts of 3H-Dex and 3H-MP were taken up by the plasma membrane (F1) fraction in control, border zone, and ischemic myocardial tissue. Lesser amounts were incorporated into the remaining cell fractions. A gradient of glucocorticoid uptake was observed that decreased from control tissue to ischemic tissue in all subcellular fractions (i.e., F1 to F5). Accordingly, supernatant fraction (S) to particulate (P) ratios of labeled glucocorticoid uptake increased from control to ischemic tissue, indicating that myocardial cell damage resulted in a decrease in glucocorticoid-binding capacity in subcellular fractions obtained from ischemic tissue. The activity of 5'-nucleotidase (5'ND), a plasma membrane marker in myocardial cells, also decreased from normal to ischemic tissue. Furthermore, we found that uptake of 3H-MP and 3H-Dex was associated with the retention of 5'ND activity in F1 fractions of both border zone and ischemic tissue. Similar protection of plasma membrane integritg occurred in the supernatant fraction as determined by changes in S/P ratios of 5'ND activity. These data provide support for the concepts that (1) plasma membrane changes occur soon after acute myocardial ischemia, and (2) the mechanism by which glucocorticoids exert a protective effect in myocardial ischemia may be related to membrane stabilization.", "PMID": 975452}
{"id": "pubmed23n0032_10259", "title": "Effects of hyperthermic stress on myocardial function during experimental coronary ischemia.", "content": "We evaluated hyperthermic influences on ischemic hearts by comparing two groups of intact working swine hearts (n = 20) made globally ischemic. Heart muscle temperature was selectively increased from 37.5 +/- 0.3 degrees C to 39.7 +/- 0.3 degrees C in one group (n = 11) by warming the coronary perfusate. Ischemia in normothermic hearts significantly (P less than 0.05) decreased mechanical function (as reflected by increases in left ventricular end-diastolic pressure [LVEDP]), myocardial oxygen consumption (MVO2), glucose uptake, glycolytic flux, free fatty acid (FFA) uptake and oxidation, and tissue stores of high energy phosphates. Hearing in ischemic hearts further depressed mechanical function at similar reductions in coronary flow and MVO2. Glucose uptake was terminally increased over normothermic values (329 vs. 221 mumol/hr per g) as was glycolytic metabolism, FFA uptake (26 vs. 17 mumol/hr per g), and FFA oxidation (21 vs. 11 mumol/hr per g). However, these changes were not translated into increased energy stores of tissue creatine phosphate and ATP. Thus, in ischemic hearts, hyperthermia neither prevented the development of mechanical deterioration nor improved oxidative phosphorylation despite increases in metabolic substrate utilization. These data suggest that in experimental global ischemia heat is an added energy drain in already burdened myocardium.", "contents": "Effects of hyperthermic stress on myocardial function during experimental coronary ischemia. We evaluated hyperthermic influences on ischemic hearts by comparing two groups of intact working swine hearts (n = 20) made globally ischemic. Heart muscle temperature was selectively increased from 37.5 +/- 0.3 degrees C to 39.7 +/- 0.3 degrees C in one group (n = 11) by warming the coronary perfusate. Ischemia in normothermic hearts significantly (P less than 0.05) decreased mechanical function (as reflected by increases in left ventricular end-diastolic pressure [LVEDP]), myocardial oxygen consumption (MVO2), glucose uptake, glycolytic flux, free fatty acid (FFA) uptake and oxidation, and tissue stores of high energy phosphates. Hearing in ischemic hearts further depressed mechanical function at similar reductions in coronary flow and MVO2. Glucose uptake was terminally increased over normothermic values (329 vs. 221 mumol/hr per g) as was glycolytic metabolism, FFA uptake (26 vs. 17 mumol/hr per g), and FFA oxidation (21 vs. 11 mumol/hr per g). However, these changes were not translated into increased energy stores of tissue creatine phosphate and ATP. Thus, in ischemic hearts, hyperthermia neither prevented the development of mechanical deterioration nor improved oxidative phosphorylation despite increases in metabolic substrate utilization. These data suggest that in experimental global ischemia heat is an added energy drain in already burdened myocardium.", "PMID": 975453}
{"id": "pubmed23n0032_10260", "title": "Changes in coronary and collateral flows and adequacy of perfusion in the dog following one and three months of circumflex occlusion.", "content": "We investigated changes in circumflex, left anterior descending (LAD), and right coronary artery flows as well as changes in collateral flows to these vessels after long-term circumflex occlusion. Coronary and collateral flows of each vessel were determined simultaneously in an isolated heart preparation in which the vasculature was maximally dilated with dipyridamole. The resistances as related to total heart weight of the circumflex, LAD, and right coronary arteries of 16 control dogs were found to be 0.59 +/- 0.06, 0.93 +/- 0.09, and 2.37 +/- 0.17 (mean +/- SEM) mm Hg/[(ml/min)/100 g], respectively. Total minimal coronary resistance was 0.21 +/- 0.01. In 10 dogs subjected to occlusion for 1 month no significant change in circumflex coronary resistance was observed, but the resistance of the unimpaired vessels decreased significantly. The resistances of the LAD and right coronary arteries were 0.66 +/-0.04 and 1.72 +/- 0.13, respectively. Both values were considerably less (P less than 0.01) than control. In nine dogs subjected to occlusion for 3 months the resistance of the unimpaired LAD and right arteries, as well as the circumflex coronary resitance, were not significantly different from control. We also found that retrograde flows for all vessels increased 7-fold after 1 month and 10.5-fold (relative to control) after 3 months of occlusion. From these data we conclude that vascular adaptations, which occurred in response to an ischemic stimulus, are responsible for the long-term regulation of the metabolic needs of the myocardium.", "contents": "Changes in coronary and collateral flows and adequacy of perfusion in the dog following one and three months of circumflex occlusion. We investigated changes in circumflex, left anterior descending (LAD), and right coronary artery flows as well as changes in collateral flows to these vessels after long-term circumflex occlusion. Coronary and collateral flows of each vessel were determined simultaneously in an isolated heart preparation in which the vasculature was maximally dilated with dipyridamole. The resistances as related to total heart weight of the circumflex, LAD, and right coronary arteries of 16 control dogs were found to be 0.59 +/- 0.06, 0.93 +/- 0.09, and 2.37 +/- 0.17 (mean +/- SEM) mm Hg/[(ml/min)/100 g], respectively. Total minimal coronary resistance was 0.21 +/- 0.01. In 10 dogs subjected to occlusion for 1 month no significant change in circumflex coronary resistance was observed, but the resistance of the unimpaired vessels decreased significantly. The resistances of the LAD and right coronary arteries were 0.66 +/-0.04 and 1.72 +/- 0.13, respectively. Both values were considerably less (P less than 0.01) than control. In nine dogs subjected to occlusion for 3 months the resistance of the unimpaired LAD and right arteries, as well as the circumflex coronary resitance, were not significantly different from control. We also found that retrograde flows for all vessels increased 7-fold after 1 month and 10.5-fold (relative to control) after 3 months of occlusion. From these data we conclude that vascular adaptations, which occurred in response to an ischemic stimulus, are responsible for the long-term regulation of the metabolic needs of the myocardium.", "PMID": 975454}
{"id": "pubmed23n0032_10261", "title": "Angiotensin II and its heptapeptide (2-8), hexapeptide (3-8), and pentapeptide (4-8) metabolites in arterial and venous blood of man.", "content": "We made separate measurements of angiotensin II (A II) and of its immunoreactive metabolites (2-8 heptapeptide, 3-8 hexapeptide, and 4-8 pentapeptide) in arterial and venous plasma from subjects with widely different plasma levels of the peptides. A II and its three metabolites were extracted from blood, separated by paper chromatography, and measured by radioimmunoassay using an A II antiserum which had a 100% cross-reaction with each metabolite. In contrast to results of previous studies, A II was found to predominate in both arterial (60-100%) and venous (55-100%) blood. The biologically active 2-8 heptapeptide metabolite accounted for only 10% of the activity in arterial plasma. Radioimmunoassay of venous plasma extracts using an A II antiserum which had a low cross-reaction with the 3-8 hexapeptide and the 4-8 pentapeptide confirmed the results obtained using the antiserum which had a 100% cross-reaction with the metabolites. We conclude that radioimmunoassay methods for measuring A II in venous blood may be more accurate and relevant than has previously been recognized. The small difference between A II concentrations in arterial and venous plasma suggests further that there may be significant conversion of angiotensin I (A I) to A II in the limb vasculature.", "contents": "Angiotensin II and its heptapeptide (2-8), hexapeptide (3-8), and pentapeptide (4-8) metabolites in arterial and venous blood of man. We made separate measurements of angiotensin II (A II) and of its immunoreactive metabolites (2-8 heptapeptide, 3-8 hexapeptide, and 4-8 pentapeptide) in arterial and venous plasma from subjects with widely different plasma levels of the peptides. A II and its three metabolites were extracted from blood, separated by paper chromatography, and measured by radioimmunoassay using an A II antiserum which had a 100% cross-reaction with each metabolite. In contrast to results of previous studies, A II was found to predominate in both arterial (60-100%) and venous (55-100%) blood. The biologically active 2-8 heptapeptide metabolite accounted for only 10% of the activity in arterial plasma. Radioimmunoassay of venous plasma extracts using an A II antiserum which had a low cross-reaction with the 3-8 hexapeptide and the 4-8 pentapeptide confirmed the results obtained using the antiserum which had a 100% cross-reaction with the metabolites. We conclude that radioimmunoassay methods for measuring A II in venous blood may be more accurate and relevant than has previously been recognized. The small difference between A II concentrations in arterial and venous plasma suggests further that there may be significant conversion of angiotensin I (A I) to A II in the limb vasculature.", "PMID": 975455}
{"id": "pubmed23n0032_10262", "title": "The myocardial energetic active state. I. Oxygen consumption during tetanus of cat papillary muscle.", "content": "The potential role of active state maintenance as a determinant of myocardial oxygen consumption (MVO2) has not been defined. Right ventricular papillary muscles from 15 cats were studied in a polarographic myograph at 23 degrees C in a Krebs-Ringer solution containing 7.5 mM Ca2+ and 10 mM caffeine. MVO2 was determined for isometric tetani at Lmax of 1-5 seconds' duration. Increases in tetanus duration related linearly to increments in both active tension time (delta active tension) and MVO2. In order to examine the oxygen cost of active state maintenance not attributable to associated tension generation, both the same isometric and 2.5- to 10.0-second lightly preloaded isotonic tetani were produced in nine muscles. For each tetanus duration the contribution throughout the contraction of developed force (preload) to MVO2 could be subtracted from overall isotonic MV02. In the absence of the MVO2 associated with force development, the active state duration was related linearly to MVO2, with a mean active state MVO2 of 2.42 +/- 0.29 nl O2/mg dry muscle/sec of isotonic tetanus; this MVO2 is 68% of the value of 3.58 +/- 0.42 nl O2/mg dry muscle/sec that was obtained for isometric tetanus at Lmax. This study identifies active state maintenance as the major determinant of MVO2 during myocardial tetanus and, furthermore, suggests the possibility that alterations in ative state intensity and duration may be the biochemical mechanism by which other determinants of MVO2 act in a more physiological setting.", "contents": "The myocardial energetic active state. I. Oxygen consumption during tetanus of cat papillary muscle. The potential role of active state maintenance as a determinant of myocardial oxygen consumption (MVO2) has not been defined. Right ventricular papillary muscles from 15 cats were studied in a polarographic myograph at 23 degrees C in a Krebs-Ringer solution containing 7.5 mM Ca2+ and 10 mM caffeine. MVO2 was determined for isometric tetani at Lmax of 1-5 seconds' duration. Increases in tetanus duration related linearly to increments in both active tension time (delta active tension) and MVO2. In order to examine the oxygen cost of active state maintenance not attributable to associated tension generation, both the same isometric and 2.5- to 10.0-second lightly preloaded isotonic tetani were produced in nine muscles. For each tetanus duration the contribution throughout the contraction of developed force (preload) to MVO2 could be subtracted from overall isotonic MV02. In the absence of the MVO2 associated with force development, the active state duration was related linearly to MVO2, with a mean active state MVO2 of 2.42 +/- 0.29 nl O2/mg dry muscle/sec of isotonic tetanus; this MVO2 is 68% of the value of 3.58 +/- 0.42 nl O2/mg dry muscle/sec that was obtained for isometric tetanus at Lmax. This study identifies active state maintenance as the major determinant of MVO2 during myocardial tetanus and, furthermore, suggests the possibility that alterations in ative state intensity and duration may be the biochemical mechanism by which other determinants of MVO2 act in a more physiological setting.", "PMID": 975456}
{"id": "pubmed23n0032_10263", "title": "The effect of an acute increase in renal perfusion pressure on sodium transport in the rat kidney.", "content": "We used micropuncture techniques to examine the intrarenal response to an acute elevation of the renal perfusion pressure. In one series of studies (epinephrine, group I) the renal perfusion pressure was acutely increased by intravenous epinephrine infusion; in another series, by bilateral carotid occlusion and vagotomy. A third series of studies (epinephrine, group II) was performed identically to the epinephrine, group I, studies except that the renal perfusion pressure was held constant during the epinephrine infusion by suprarenal aortic constriction. After epinephrine infusion (group I) and following bilateral carotid occlusion and vagotomy the renal perfusion pressure increased, from 119 +/- 1.0 (SEM) to 166 +/- 1.85 mm Hg and from 122 +/- 5.9 to 168 +/- 3.1 mm Hg, respectively. Fractional sodium excretion rose from 2.31 +/- 0.34% to 5.09 +/- 0.58% (P less than 0.001) after epinephrine and from 1.80 +/- 0.71 to 6.40 +/- 1.0% (P less than 0.01) following carotid occlusion and vagotomy. In neither study, however, did we find that the increase in renal perfusion pressure changed the glomerular filtration rate (GFR) (both kidneys) or fractional sodium delivery from the superficial cortical late distal tubule. Furthermore, we found that epinephrine infusion at a constant renal perfusion pressure (epinephrine, group II) did not affect fractional sodium excretion, although a small, but significant, decrease in the GFR and sodium delivery from the superficial late distal tubule occurred. These data suggest that the natriuresis which follows an acute elevation of the renal perfusion pressure cannot be attributed to enhanced sodium delivery from superficial nephrons but must result from (1) inhibition of sodium reabsorption in inner cortical nephrons or (2) an effect on sodium transport in the collecting system.", "contents": "The effect of an acute increase in renal perfusion pressure on sodium transport in the rat kidney. We used micropuncture techniques to examine the intrarenal response to an acute elevation of the renal perfusion pressure. In one series of studies (epinephrine, group I) the renal perfusion pressure was acutely increased by intravenous epinephrine infusion; in another series, by bilateral carotid occlusion and vagotomy. A third series of studies (epinephrine, group II) was performed identically to the epinephrine, group I, studies except that the renal perfusion pressure was held constant during the epinephrine infusion by suprarenal aortic constriction. After epinephrine infusion (group I) and following bilateral carotid occlusion and vagotomy the renal perfusion pressure increased, from 119 +/- 1.0 (SEM) to 166 +/- 1.85 mm Hg and from 122 +/- 5.9 to 168 +/- 3.1 mm Hg, respectively. Fractional sodium excretion rose from 2.31 +/- 0.34% to 5.09 +/- 0.58% (P less than 0.001) after epinephrine and from 1.80 +/- 0.71 to 6.40 +/- 1.0% (P less than 0.01) following carotid occlusion and vagotomy. In neither study, however, did we find that the increase in renal perfusion pressure changed the glomerular filtration rate (GFR) (both kidneys) or fractional sodium delivery from the superficial cortical late distal tubule. Furthermore, we found that epinephrine infusion at a constant renal perfusion pressure (epinephrine, group II) did not affect fractional sodium excretion, although a small, but significant, decrease in the GFR and sodium delivery from the superficial late distal tubule occurred. These data suggest that the natriuresis which follows an acute elevation of the renal perfusion pressure cannot be attributed to enhanced sodium delivery from superficial nephrons but must result from (1) inhibition of sodium reabsorption in inner cortical nephrons or (2) an effect on sodium transport in the collecting system.", "PMID": 975457}
{"id": "pubmed23n0032_10264", "title": "Reflex vascular capacity reduction in the dog.", "content": "The maximum degree and time course of active reflex venoconstriction in chloralose-anesthetized dogs was studied. The mean circulatory filling pressure (Pmc) was measured by fibrillating the heart and rapidly pumping blood from the aorta to the vena cava until the systemic arterial pressure equaled the central venous pressure. Ventricular fibrillation was continued for 1 minute and was assumed to induce maximal sympathetic discharge to the capacity vessels. Blood was removed to maintain the Pmc constant at the level determined at 8 seconds after the start of fibrillation. It was necessary to remove 13.8 ml/kg by the end of 1 minute. After autonomic nervous system blockade by hexamethonium only 4.8 ml/kg were removed to hold the Pmc constant. Thus, in conclusion, the maximal degree of active venoconstriction induced by circulatory arrest was 9.0 ml/kg during the 1st minute of cardiac fibrillation. A basal capacity vessel tone was present, equivalent to 10 ml/kg under the experimental conditions used.", "contents": "Reflex vascular capacity reduction in the dog. The maximum degree and time course of active reflex venoconstriction in chloralose-anesthetized dogs was studied. The mean circulatory filling pressure (Pmc) was measured by fibrillating the heart and rapidly pumping blood from the aorta to the vena cava until the systemic arterial pressure equaled the central venous pressure. Ventricular fibrillation was continued for 1 minute and was assumed to induce maximal sympathetic discharge to the capacity vessels. Blood was removed to maintain the Pmc constant at the level determined at 8 seconds after the start of fibrillation. It was necessary to remove 13.8 ml/kg by the end of 1 minute. After autonomic nervous system blockade by hexamethonium only 4.8 ml/kg were removed to hold the Pmc constant. Thus, in conclusion, the maximal degree of active venoconstriction induced by circulatory arrest was 9.0 ml/kg during the 1st minute of cardiac fibrillation. A basal capacity vessel tone was present, equivalent to 10 ml/kg under the experimental conditions used.", "PMID": 975458}
{"id": "pubmed23n0032_10265", "title": "A delayed suppression of the renin-aldosterone axis following saline infusion in human hypertension.", "content": "The purpose of this study was to compare the acute suppressibility of the renin-angiotensin-aldosterone (RAA) axis in normotensive (n = 23) and essential hypertensive (n = 62) subjects. Only those hypertensive subjects with normal plasma renin activity (PRA) levels (sodium restricted, upright) were included in the study. Acute suppression of the RAA axis was determined by measuring PRA, plasma angiotensin II (A II), and plasma aldosterone (PA) at frequent intervals during the infusion of isotonic saline (500 ml/hour for 6 hours). Although all parameters fell significantly from control levels by 20-30 minutes in the normotensive subjects, we found that 60% of the hypertensive subjects showed no significant decline in PRA or PA until 120-240 minutes after beginning the infusion. The other hypertensive subjects showed normal RAA suppression. In addition, while there were no significant differences between the three groups in control PRA or PA levels, we found that the PA levels from 30 to 240 minutes during the saline were significantly higher (P less than 0.01) in the hypertensive subjects with delayed suppression. That there were two distinct populations in the hypertensive group was suggested by the bimodality of the frequency response curve, with peaks occurring at 30 and 240 minutes. These studies indicate an abnormality in the acute suppression of the RAA axis in a substantial proportion of subjects with normal renin essential hypertension. Since previous studies in normal subjects have reported that the early phase of response to saline infusion is related to the sodium ion per se and not to intravascular volume expansion, we have come to the conclusion that the present data are consistent with the hypothesis that the delayed suppression hypertensive group has a diminished ability to respond to the sodium ion.", "contents": "A delayed suppression of the renin-aldosterone axis following saline infusion in human hypertension. The purpose of this study was to compare the acute suppressibility of the renin-angiotensin-aldosterone (RAA) axis in normotensive (n = 23) and essential hypertensive (n = 62) subjects. Only those hypertensive subjects with normal plasma renin activity (PRA) levels (sodium restricted, upright) were included in the study. Acute suppression of the RAA axis was determined by measuring PRA, plasma angiotensin II (A II), and plasma aldosterone (PA) at frequent intervals during the infusion of isotonic saline (500 ml/hour for 6 hours). Although all parameters fell significantly from control levels by 20-30 minutes in the normotensive subjects, we found that 60% of the hypertensive subjects showed no significant decline in PRA or PA until 120-240 minutes after beginning the infusion. The other hypertensive subjects showed normal RAA suppression. In addition, while there were no significant differences between the three groups in control PRA or PA levels, we found that the PA levels from 30 to 240 minutes during the saline were significantly higher (P less than 0.01) in the hypertensive subjects with delayed suppression. That there were two distinct populations in the hypertensive group was suggested by the bimodality of the frequency response curve, with peaks occurring at 30 and 240 minutes. These studies indicate an abnormality in the acute suppression of the RAA axis in a substantial proportion of subjects with normal renin essential hypertension. Since previous studies in normal subjects have reported that the early phase of response to saline infusion is related to the sodium ion per se and not to intravascular volume expansion, we have come to the conclusion that the present data are consistent with the hypothesis that the delayed suppression hypertensive group has a diminished ability to respond to the sodium ion.", "PMID": 975459}
{"id": "pubmed23n0032_10266", "title": "Depression and enhancement of baroreceptor pressor response in cats after intracerebroventricular injection of noradrenergic blocking agents: dependence on supracollicular areas of the brain.", "content": "The alpha-adrenergic blocking drugs, phentolamine and Hydergine, both act centrally at different sites to depress and enhance the pressor and sympathetic nerve response to decreased baroreceptor afferent input in anesthetized cats. Depression of the rise in blood pressure and sympathetic nerve discharge during bilateral carotid occlusion (BCO) followed injection of the agents into the 4th cerebral ventricle when the brain was intact but not when connections were interrupted at the midcollicular level by transection or lesion. Enhancement of responses occurred when drug distribution was confined to the brain rostral to the midcollicular level via injection into the 3rd cerebral ventricle with the cerebral aqueduct cannulated. Both agents decreased resting blood pressure and Hydergine decreased heart rate in intact and decerebrate preparations but not in 3rd ventricle-cerebral aqueduct experiments. We found that pretreatment with the noradrenergic precursor. L-dopa consistently prevented depression by phentolamine but was less effective against Hydergine. The results indicate that mechanisms which enhance and suppress the baroreceptor pressor response are normally operative in anesthetized cats and, furthermore, that neural pathways mediating the effects are ones connecting the caudal brainstem with supracollicular levels of the brain. It is further suggested that the pathways may be noradrenergic.", "contents": "Depression and enhancement of baroreceptor pressor response in cats after intracerebroventricular injection of noradrenergic blocking agents: dependence on supracollicular areas of the brain. The alpha-adrenergic blocking drugs, phentolamine and Hydergine, both act centrally at different sites to depress and enhance the pressor and sympathetic nerve response to decreased baroreceptor afferent input in anesthetized cats. Depression of the rise in blood pressure and sympathetic nerve discharge during bilateral carotid occlusion (BCO) followed injection of the agents into the 4th cerebral ventricle when the brain was intact but not when connections were interrupted at the midcollicular level by transection or lesion. Enhancement of responses occurred when drug distribution was confined to the brain rostral to the midcollicular level via injection into the 3rd cerebral ventricle with the cerebral aqueduct cannulated. Both agents decreased resting blood pressure and Hydergine decreased heart rate in intact and decerebrate preparations but not in 3rd ventricle-cerebral aqueduct experiments. We found that pretreatment with the noradrenergic precursor. L-dopa consistently prevented depression by phentolamine but was less effective against Hydergine. The results indicate that mechanisms which enhance and suppress the baroreceptor pressor response are normally operative in anesthetized cats and, furthermore, that neural pathways mediating the effects are ones connecting the caudal brainstem with supracollicular levels of the brain. It is further suggested that the pathways may be noradrenergic.", "PMID": 975460}
{"id": "pubmed23n0032_10267", "title": "Effect of quinidine and temperature on sodium uptake and contraction frequency of cultured rat myocardial cells.", "content": "The effects of quinidine and temperature on Na influx and contraction frequency of synchronously contracting rat myocardial cells in monolayer cultures were studied. Quinidine (10(-6) M to 10(-1) M) produced a prompt reduction in Na influx, maximum after 30 seconds of exposure, and dose-dependent along a sigmoid log dose-response curve. At 37 degrees C, Na influx (mumol/10(11) cells per sec) decreased from 30.19 to 24.70 (P less than 0.001) and 10.49 (P less than 0.001) on exposure to quinidine, 10(-6) and 10(-2) M, respectively. Simultaneously the contraction frequency decreased from a control of 120/min to 105/min and 48/min with 10(-6) M and 5 X 10(-4) M quinidine. At higher concentrations spontaneous contractions ceased. The effects on Na influx and contraction were reversible by washing the cells free of the drug (30 seconds). A temperature-dependent decrease in the Na influx between 37 degrees C and 22 degrees C also induced a decrease in contraction frequency. Between 25 degrees C and 35 degrees C the Q10 values for Na influx and contraction frequency were 2.41 and 2.44 respectively. Under all conditions tested there was a constant linear relationship (r = 0.98) between Na influx and contraction frequency for all values of Na influx greater than 11.82 mumol/10(11) cells per sec. Na influx and contraction frequency were insensitive to tetrodotoxin (10(-5) g/ml) but very sensitive to verapamil and to changes in extracellular Na. Quinidine affected only the verapamil-sensitive Na influx. The results indicate a close relationship between verapamil-sensitive inward Na movement and automaticity in these cells and demonstrate that the quinidine-induced changes in automaticity are closely linked to the effect on Na influx.", "contents": "Effect of quinidine and temperature on sodium uptake and contraction frequency of cultured rat myocardial cells. The effects of quinidine and temperature on Na influx and contraction frequency of synchronously contracting rat myocardial cells in monolayer cultures were studied. Quinidine (10(-6) M to 10(-1) M) produced a prompt reduction in Na influx, maximum after 30 seconds of exposure, and dose-dependent along a sigmoid log dose-response curve. At 37 degrees C, Na influx (mumol/10(11) cells per sec) decreased from 30.19 to 24.70 (P less than 0.001) and 10.49 (P less than 0.001) on exposure to quinidine, 10(-6) and 10(-2) M, respectively. Simultaneously the contraction frequency decreased from a control of 120/min to 105/min and 48/min with 10(-6) M and 5 X 10(-4) M quinidine. At higher concentrations spontaneous contractions ceased. The effects on Na influx and contraction were reversible by washing the cells free of the drug (30 seconds). A temperature-dependent decrease in the Na influx between 37 degrees C and 22 degrees C also induced a decrease in contraction frequency. Between 25 degrees C and 35 degrees C the Q10 values for Na influx and contraction frequency were 2.41 and 2.44 respectively. Under all conditions tested there was a constant linear relationship (r = 0.98) between Na influx and contraction frequency for all values of Na influx greater than 11.82 mumol/10(11) cells per sec. Na influx and contraction frequency were insensitive to tetrodotoxin (10(-5) g/ml) but very sensitive to verapamil and to changes in extracellular Na. Quinidine affected only the verapamil-sensitive Na influx. The results indicate a close relationship between verapamil-sensitive inward Na movement and automaticity in these cells and demonstrate that the quinidine-induced changes in automaticity are closely linked to the effect on Na influx.", "PMID": 975461}
{"id": "pubmed23n0032_10268", "title": "Inhibition of feline collateral vessel development following experimental thrombolic occlusion.", "content": "We compared development of feline hindlimb collateral circulation after acute occlusion of the terminal aorta by ligation, thrombus formation, and formation of a \"closed\" aortic loop containing thromboplastin. Collateral circulation development was assessed by aortograms, scintillation scans, neurological signs following occlusion, measurement of hindlimb muscle blood flow, and forelimb and hindlimb temperature. In cats in which aortic occlusion was the result of ligation or thromboplastin in the aortic loop, paralysis was not evident. Aortograms and scintillation scans indicated hindlimb blood flow. Both muscle temperature and blood flow data indicated that the return of blood flow was rapid. The 5th lumbar artery appears to be the origin of the collateral vessels. The mid-zone component is a dorsal and ventral vertebral route and an epaxial muscle route. The reentry components are the 6th or 7th lumbar arteries. The collateral vessels arise from preexisting collateral vessels. Of those cats in which aortic occlusion was the result of a thrombus, all exhibited paralysis. Aortograms, scintillation scans, muscle temperature, and hindlimb blood flow data indicated reduced hindlimb blood flow. The results suggest that the thrombus has an inhibitory effect on the development of collateral circulation.", "contents": "Inhibition of feline collateral vessel development following experimental thrombolic occlusion. We compared development of feline hindlimb collateral circulation after acute occlusion of the terminal aorta by ligation, thrombus formation, and formation of a \"closed\" aortic loop containing thromboplastin. Collateral circulation development was assessed by aortograms, scintillation scans, neurological signs following occlusion, measurement of hindlimb muscle blood flow, and forelimb and hindlimb temperature. In cats in which aortic occlusion was the result of ligation or thromboplastin in the aortic loop, paralysis was not evident. Aortograms and scintillation scans indicated hindlimb blood flow. Both muscle temperature and blood flow data indicated that the return of blood flow was rapid. The 5th lumbar artery appears to be the origin of the collateral vessels. The mid-zone component is a dorsal and ventral vertebral route and an epaxial muscle route. The reentry components are the 6th or 7th lumbar arteries. The collateral vessels arise from preexisting collateral vessels. Of those cats in which aortic occlusion was the result of a thrombus, all exhibited paralysis. Aortograms, scintillation scans, muscle temperature, and hindlimb blood flow data indicated reduced hindlimb blood flow. The results suggest that the thrombus has an inhibitory effect on the development of collateral circulation.", "PMID": 975462}
{"id": "pubmed23n0032_10269", "title": "Idiopathic prolapsed mitral leaflet syndrome. Angiographic-clinical correlations.", "content": "Angiographic clinical correlations were made in 59 patients with prolapsed mitral leaflet syndrome. Eight had nonejection systolic clicks (group I), 20 had early, mid or late systolic murmurs with or without a systolic click (group II), and 31 had pansystolic murmurs (group III). Isolated prolapse of posterior leaflet (PL) scallops occurred in 42 and 17 had combined leaflet prolapse. The study demonstrated the following: (I) Group II patients usually had isolated PL prolapse with a predominant biscallop involvement while a high incidence of triple scallop prolapse and combined mitral leaflet prolapse occurred in group III. (II) Severe mitral regurgitation and a greater incidence of atrial fibrillation were seen in patients with triscallop prolapse and combined mitral leaflet prolapse. Mitral regurgitation was milder in patients with single and biscallop prolapse and, when severe, was associated with ruptured chordae. (III) ST-T wave abnormalities in the  inferior leads were most frequent in patients with isolated PL prolapse. (IV) Systolic and diastolic asynergy occurred in 41 patients, most frequently in group II but also relatively frequently in group III (19 of 31). Segmental anterior dysfunction with normal ejection fraction was found in 18 patients, of whom 13 had early anterior wall relaxation. (V) Patients without asynergy were slightly older than those with it. More in the former group had severe mitral regurgitation and were clinically disabled from it.", "contents": "Idiopathic prolapsed mitral leaflet syndrome. Angiographic-clinical correlations. Angiographic clinical correlations were made in 59 patients with prolapsed mitral leaflet syndrome. Eight had nonejection systolic clicks (group I), 20 had early, mid or late systolic murmurs with or without a systolic click (group II), and 31 had pansystolic murmurs (group III). Isolated prolapse of posterior leaflet (PL) scallops occurred in 42 and 17 had combined leaflet prolapse. The study demonstrated the following: (I) Group II patients usually had isolated PL prolapse with a predominant biscallop involvement while a high incidence of triple scallop prolapse and combined mitral leaflet prolapse occurred in group III. (II) Severe mitral regurgitation and a greater incidence of atrial fibrillation were seen in patients with triscallop prolapse and combined mitral leaflet prolapse. Mitral regurgitation was milder in patients with single and biscallop prolapse and, when severe, was associated with ruptured chordae. (III) ST-T wave abnormalities in the  inferior leads were most frequent in patients with isolated PL prolapse. (IV) Systolic and diastolic asynergy occurred in 41 patients, most frequently in group II but also relatively frequently in group III (19 of 31). Segmental anterior dysfunction with normal ejection fraction was found in 18 patients, of whom 13 had early anterior wall relaxation. (V) Patients without asynergy were slightly older than those with it. More in the former group had severe mitral regurgitation and were clinically disabled from it.", "PMID": 975464}
{"id": "pubmed23n0032_10270", "title": "Mitral valve prolapse. Two-dimensional echocardiographic and angiographic correlation.", "content": "In order to define baseline descriptive criteria for the diagnosis of mitral valve prolapse with cross-sectional echocardiography, 49 patients undergoing catheterization were examined by a real-time, two-dimensional phased array echocardiographic imaging system. Angiography was used to separate patients into two distinct groups: 15 with normal mitral valve function and 34 with definite mitral valve prolapse. Systolic mitral leaflet and annulus motion were then observed in each patient and similarities and differences were noted between the two groups of patients. Correlative M-mode echocardiographic data were available in 37 patients. Certain two-dimensional echocardiographic findings restricted to the angiographically proven mitral valve prolapse group were defined: 1) posteriorly displaced coaptation of the leaflets, 2) systolic superior movement of one or both mitral leaflets above the level of the mitral ring, and 3) a systolic curling motion of the posterior mitral ring on its adjacent myocardium. One or more of these abnormalities were found in all 34 patients with angiographic mitral valve prolapse. When mitral valve prolapse does occur, the results of two-dimensional echocardiography would suggest that both leaflets are usually involved.", "contents": "Mitral valve prolapse. Two-dimensional echocardiographic and angiographic correlation. In order to define baseline descriptive criteria for the diagnosis of mitral valve prolapse with cross-sectional echocardiography, 49 patients undergoing catheterization were examined by a real-time, two-dimensional phased array echocardiographic imaging system. Angiography was used to separate patients into two distinct groups: 15 with normal mitral valve function and 34 with definite mitral valve prolapse. Systolic mitral leaflet and annulus motion were then observed in each patient and similarities and differences were noted between the two groups of patients. Correlative M-mode echocardiographic data were available in 37 patients. Certain two-dimensional echocardiographic findings restricted to the angiographically proven mitral valve prolapse group were defined: 1) posteriorly displaced coaptation of the leaflets, 2) systolic superior movement of one or both mitral leaflets above the level of the mitral ring, and 3) a systolic curling motion of the posterior mitral ring on its adjacent myocardium. One or more of these abnormalities were found in all 34 patients with angiographic mitral valve prolapse. When mitral valve prolapse does occur, the results of two-dimensional echocardiography would suggest that both leaflets are usually involved.", "PMID": 975465}
{"id": "pubmed23n0032_10271", "title": "Distribution of coronary artery disease. Prediction by echocardiography.", "content": "To assess the sensitivity of standard echocardiography in detecting ventricular motion abnormalities in patients with coronary artery disease (CAD) without prior myocardial infarction, 56 consecutive patients with a history of angina pectoris were studied during an angina-free period. In the 48 patients with adequate echocardiograms, the amplitude of septal and posterior wall motion in the high, mid, and low left ventricle was determined and used to predict prospectively in a blinded fashion the sites of angiographically-determined CAD. Twenty-eight of 35 patients (80%) with disease of the left anterior descending artery (LAD) had diminished interventricular septal motion (P less than 0.001) and 14 of 27 patients (52%) with disease of posterior vessels had diminished posterior wall motion on echocardiogram. When abnormalties of echocardiographic wall motion were compared with left ventriculography, the results were similar. Echocardiography may aid in predicting the presence and distribution of CAD, especially LAD disease.", "contents": "Distribution of coronary artery disease. Prediction by echocardiography. To assess the sensitivity of standard echocardiography in detecting ventricular motion abnormalities in patients with coronary artery disease (CAD) without prior myocardial infarction, 56 consecutive patients with a history of angina pectoris were studied during an angina-free period. In the 48 patients with adequate echocardiograms, the amplitude of septal and posterior wall motion in the high, mid, and low left ventricle was determined and used to predict prospectively in a blinded fashion the sites of angiographically-determined CAD. Twenty-eight of 35 patients (80%) with disease of the left anterior descending artery (LAD) had diminished interventricular septal motion (P less than 0.001) and 14 of 27 patients (52%) with disease of posterior vessels had diminished posterior wall motion on echocardiogram. When abnormalties of echocardiographic wall motion were compared with left ventriculography, the results were similar. Echocardiography may aid in predicting the presence and distribution of CAD, especially LAD disease.", "PMID": 975466}
{"id": "pubmed23n0032_10272", "title": "Hemodynamic correlates of the normal aortic valve echogram. A study of sound, flow, and motion.", "content": "The aortic valve echogram was recorded in open chest dogs simultaneously with aortic flow, acceleration of flow, aortic and left ventricular pressures, and intracardiac phonocardiograms. Comparison of echographic with hemodynamic data showed the following: The aortic valve started its opening with the onset of flow, at the same point that left ventricular pressure exceeded aortic pressure. Complete valve opening preceded peak aortic flow by an average of 43 msec and the cusps started to move toward closure while flow was still accelerating. Final closure of the valve was achieved at the time of zero flow and preceded the aortic second sound by 4-10 msec. The echographically determined \"valve orifice area\" correlated well with aortic stroke volume (r = 0.94). The intensity of the aortic first sound was related to peak acceleration of aortic flow. The intensity of the second sound was not related to the amplitude of cusp motion but correlated well with the aortic pressure at the time of closure and with peak flow deceleration.", "contents": "Hemodynamic correlates of the normal aortic valve echogram. A study of sound, flow, and motion. The aortic valve echogram was recorded in open chest dogs simultaneously with aortic flow, acceleration of flow, aortic and left ventricular pressures, and intracardiac phonocardiograms. Comparison of echographic with hemodynamic data showed the following: The aortic valve started its opening with the onset of flow, at the same point that left ventricular pressure exceeded aortic pressure. Complete valve opening preceded peak aortic flow by an average of 43 msec and the cusps started to move toward closure while flow was still accelerating. Final closure of the valve was achieved at the time of zero flow and preceded the aortic second sound by 4-10 msec. The echographically determined \"valve orifice area\" correlated well with aortic stroke volume (r = 0.94). The intensity of the aortic first sound was related to peak acceleration of aortic flow. The intensity of the second sound was not related to the amplitude of cusp motion but correlated well with the aortic pressure at the time of closure and with peak flow deceleration.", "PMID": 975467}
{"id": "pubmed23n0032_10273", "title": "Echocardiographic measurement of cardiac output using the mitral valve and aortic root echo.", "content": "The current echo method for measuring cardiac output (CO) has various technical limitations and is based on the assumptions that the left ventricle is not dilated and that it contracts uniformly. We have taken a different approach to measuring CO by echo: CO = cross-sectional area of aortic root X left ventricular ejection time X mean aortic flow velocity X heart rate. Using the Fick method, 34 measurements of CO were made in patients with normal aortic and mitral valves, with simultaneous echo recordings from the aortic and mitral valves. The systolic closure slope of the anterior mitral valve leaflet was assumed to represent the mean aortic flow velocity. CO by Fick and the proposed echo method correlated well, both in patients with (r = 0.94) and those without (r = 0.87) asynergy and the overall correlation was strong (r = 0.90). Using the conventional echo and Fick methods, a much weaker association was found between the two measurements in those patients with asynergy (r = 0.44) as opposed to those without asynergy (r = 0.77).", "contents": "Echocardiographic measurement of cardiac output using the mitral valve and aortic root echo. The current echo method for measuring cardiac output (CO) has various technical limitations and is based on the assumptions that the left ventricle is not dilated and that it contracts uniformly. We have taken a different approach to measuring CO by echo: CO = cross-sectional area of aortic root X left ventricular ejection time X mean aortic flow velocity X heart rate. Using the Fick method, 34 measurements of CO were made in patients with normal aortic and mitral valves, with simultaneous echo recordings from the aortic and mitral valves. The systolic closure slope of the anterior mitral valve leaflet was assumed to represent the mean aortic flow velocity. CO by Fick and the proposed echo method correlated well, both in patients with (r = 0.94) and those without (r = 0.87) asynergy and the overall correlation was strong (r = 0.90). Using the conventional echo and Fick methods, a much weaker association was found between the two measurements in those patients with asynergy (r = 0.44) as opposed to those without asynergy (r = 0.77).", "PMID": 975468}
{"id": "pubmed23n0032_10274", "title": "The posterior aortic wall echocardiogram. Its relationship to left atrial volume change.", "content": "The normal posterior aortic wall echocardiogram shows anterior motion during left ventricular systole and predominantly posterior motion in three phases during left ventricular diastole. In six patients undergoing simultaneous left atrial angiograms and posterior aortic wall echocardiograms, there was excellent correlation between the posterior aortic wall motion and the change in the left atrial angiographic area showing the value of the posterior aortic wall echocardiogram in describing the left atrial volume curve. Left atrial and left ventricular pressures were measured with manometer tip catheters and correlated with simultaneous posterior aortic wall and mitral valve echocardiograms in four patients with atrial septal defects. These echocardiographic, angiographic, and hemodynamic correlations, as well as other evidence reported in this paper suggest that a major portion of posterior aortic wall motion is related to left atrial events and describes the left atrial volume curve.", "contents": "The posterior aortic wall echocardiogram. Its relationship to left atrial volume change. The normal posterior aortic wall echocardiogram shows anterior motion during left ventricular systole and predominantly posterior motion in three phases during left ventricular diastole. In six patients undergoing simultaneous left atrial angiograms and posterior aortic wall echocardiograms, there was excellent correlation between the posterior aortic wall motion and the change in the left atrial angiographic area showing the value of the posterior aortic wall echocardiogram in describing the left atrial volume curve. Left atrial and left ventricular pressures were measured with manometer tip catheters and correlated with simultaneous posterior aortic wall and mitral valve echocardiograms in four patients with atrial septal defects. These echocardiographic, angiographic, and hemodynamic correlations, as well as other evidence reported in this paper suggest that a major portion of posterior aortic wall motion is related to left atrial events and describes the left atrial volume curve.", "PMID": 975469}
{"id": "pubmed23n0032_10275", "title": "The isovolumic contraction time of the left ventricle. An echographic study.", "content": "The echographic isovolumic contraction time (ICT) of the left ventricle (LV) was measured in order to assess alterations of LV performance in children with various forms of cardiac disease. The echographic ICT was defined as the interval between coaptation of the anterior and posterior mitral valve leaflets and aortic cusp opening. Four groups of patients were evaluated: normal (48); LV myocardial disease (20); patent ductus arterosus (PDA) (19); and aortic stenosis (AS) (15). In normal children ICT shortened with decreasing age and increasing heart rate. Isovolumic contraction time was more influenced by heart rate (HR) than age, but the relationship was still weak (r = 0.61). In patients with LV myocardial disease ICT was markedly prolonged, while in children with patent ductus arteriosus and aortic stenosis it was shortened as compared with normal children (P less than 0.01).", "contents": "The isovolumic contraction time of the left ventricle. An echographic study. The echographic isovolumic contraction time (ICT) of the left ventricle (LV) was measured in order to assess alterations of LV performance in children with various forms of cardiac disease. The echographic ICT was defined as the interval between coaptation of the anterior and posterior mitral valve leaflets and aortic cusp opening. Four groups of patients were evaluated: normal (48); LV myocardial disease (20); patent ductus arterosus (PDA) (19); and aortic stenosis (AS) (15). In normal children ICT shortened with decreasing age and increasing heart rate. Isovolumic contraction time was more influenced by heart rate (HR) than age, but the relationship was still weak (r = 0.61). In patients with LV myocardial disease ICT was markedly prolonged, while in children with patent ductus arteriosus and aortic stenosis it was shortened as compared with normal children (P less than 0.01).", "PMID": 975470}
{"id": "pubmed23n0032_10276", "title": "ST-segment variations after acute myocardial infarction. Relationship to clinical status.", "content": "The degree of vectorcardiographic ST-segment elevation was employed as an index of myocardial ischemic injury in a study of 27 patients after acute myocardial infarction (AMI). The ST-segment vector magnitude (STVM) was derived from the continuously recorded modified Frank vectorcardiogram and was plotted serially by hours after onset of AMI. The STVM in normal subjects was 51.1 +/- 7.1 muV (mean +/- SE). A standard deviation of the pooled variance of 15.2 muV was obtained in a group of control patients and a change of more than 2 SD (greater than 30 muV) in an individual STVM was considered to be significant. The STVM progressively decreased in patients who survived without clinical complications while it remained elevated in those with congestive heart failure. A modest, sustained re-elevation of STVM was observed in patients who developed pericarditis, and a significant late average increase of 64 muV occurred in survivors with infarct extension. In contrast, STVM underwent a major increase in patients who died. In five of these six patients without associated pericarditis a mean increase of 164 muV was recorded in the last 5-12 hours of life. While death was clinically predictable in two patients with cardiogenic shock, it was not so for the four other patients who died. Thus, major increases in STVM frequently suggested significant new ischemic injury and were often premonitory to sudden death after AMI. The increases preceding death implied that not only ventricular extopy but also lethal conduction abnormalities after AMI might be ischemia-related.", "contents": "ST-segment variations after acute myocardial infarction. Relationship to clinical status. The degree of vectorcardiographic ST-segment elevation was employed as an index of myocardial ischemic injury in a study of 27 patients after acute myocardial infarction (AMI). The ST-segment vector magnitude (STVM) was derived from the continuously recorded modified Frank vectorcardiogram and was plotted serially by hours after onset of AMI. The STVM in normal subjects was 51.1 +/- 7.1 muV (mean +/- SE). A standard deviation of the pooled variance of 15.2 muV was obtained in a group of control patients and a change of more than 2 SD (greater than 30 muV) in an individual STVM was considered to be significant. The STVM progressively decreased in patients who survived without clinical complications while it remained elevated in those with congestive heart failure. A modest, sustained re-elevation of STVM was observed in patients who developed pericarditis, and a significant late average increase of 64 muV occurred in survivors with infarct extension. In contrast, STVM underwent a major increase in patients who died. In five of these six patients without associated pericarditis a mean increase of 164 muV was recorded in the last 5-12 hours of life. While death was clinically predictable in two patients with cardiogenic shock, it was not so for the four other patients who died. Thus, major increases in STVM frequently suggested significant new ischemic injury and were often premonitory to sudden death after AMI. The increases preceding death implied that not only ventricular extopy but also lethal conduction abnormalities after AMI might be ischemia-related.", "PMID": 975471}
{"id": "pubmed23n0032_10277", "title": "Electrophysiologic properties and response to pharmacologic agents of fibers from diseased human atria.", "content": "We used standard microelectrode techniques to record action potentials of human right atrial fibers obtained during cardiac surgery, and correlated these potentials with clinical and preoperative ECG data. Human atrial fibers were classified as follows: Group A (ten patients) had a maximum diastolic potential (MDP) of -71.4 +/- 5.1mV (mean +/- SD), and action potentials that were primarily fast responses. These atria were normal or slightly dilated. In group B (12 patients) MDP was 50.3 +/- 5.7 mV; action potentials were slow responses and the atria were moderately to markedly dilated. Atrial arrhythmias occurred in four group B and no group A patients. The ECG revealed a signficant difference (P less than 0.005) in P wave duration: group A, 89 +/- 3.0 msec; group B, 111 +/- 6.0 msec. Verapamil, 0.1 mg/L, markedly depressed only the action potential plateau of group A. Procainamide 1-100 mg/L had equivalent effects on fibers of both groups A and B, effects which were small at dosages of less than 40 mg/L. Procainamide did not depress slow response automaticity, but verapamil (0.1-1 mg/L) did.", "contents": "Electrophysiologic properties and response to pharmacologic agents of fibers from diseased human atria. We used standard microelectrode techniques to record action potentials of human right atrial fibers obtained during cardiac surgery, and correlated these potentials with clinical and preoperative ECG data. Human atrial fibers were classified as follows: Group A (ten patients) had a maximum diastolic potential (MDP) of -71.4 +/- 5.1mV (mean +/- SD), and action potentials that were primarily fast responses. These atria were normal or slightly dilated. In group B (12 patients) MDP was 50.3 +/- 5.7 mV; action potentials were slow responses and the atria were moderately to markedly dilated. Atrial arrhythmias occurred in four group B and no group A patients. The ECG revealed a signficant difference (P less than 0.005) in P wave duration: group A, 89 +/- 3.0 msec; group B, 111 +/- 6.0 msec. Verapamil, 0.1 mg/L, markedly depressed only the action potential plateau of group A. Procainamide 1-100 mg/L had equivalent effects on fibers of both groups A and B, effects which were small at dosages of less than 40 mg/L. Procainamide did not depress slow response automaticity, but verapamil (0.1-1 mg/L) did.", "PMID": 975472}
{"id": "pubmed23n0032_10278", "title": "The natural history of trifascicular disease following permanent pacemaker implantation. Significance of continuing changes in atrioventricular conduction.", "content": "Seventy-two patients with trifasicular disease were followed for an average of 40 months following permanent pacemaker insertion. The indications for pacemaker insertion were either electrocardiographic evidence of complete heart block with a wide QRS escape complex or a pattern of bifasicular block with either periods of Mobitz type II atrioventricular (A-V) block or a documented history of syncope. The patients were then divided into three groups depending on subsequent change in A-V conduction. There were 31 (43%) patients with no change in A-V conduction, 17 (24%) with increasing A-V block, and 24 (33%) with decreasing A-V block. The characteristics of these three groups, including age and sex distribution, were compared and found to be similar. The incidence of previous transmural myocardial infarction as determined by electrocardiographic criteria was higher in the group with decreasing block. Survival curves showed a significantly decreased probablity of surviving for those with decreasing block compared to both those with increasing block and those with no charge in conduction (P less than 0.03). We conclude that the probability of long-term survival was less in the group with decreasing block. This finding may be related to the greater prevalence of coronary heart disease in the patients.", "contents": "The natural history of trifascicular disease following permanent pacemaker implantation. Significance of continuing changes in atrioventricular conduction. Seventy-two patients with trifasicular disease were followed for an average of 40 months following permanent pacemaker insertion. The indications for pacemaker insertion were either electrocardiographic evidence of complete heart block with a wide QRS escape complex or a pattern of bifasicular block with either periods of Mobitz type II atrioventricular (A-V) block or a documented history of syncope. The patients were then divided into three groups depending on subsequent change in A-V conduction. There were 31 (43%) patients with no change in A-V conduction, 17 (24%) with increasing A-V block, and 24 (33%) with decreasing A-V block. The characteristics of these three groups, including age and sex distribution, were compared and found to be similar. The incidence of previous transmural myocardial infarction as determined by electrocardiographic criteria was higher in the group with decreasing block. Survival curves showed a significantly decreased probablity of surviving for those with decreasing block compared to both those with increasing block and those with no charge in conduction (P less than 0.03). We conclude that the probability of long-term survival was less in the group with decreasing block. This finding may be related to the greater prevalence of coronary heart disease in the patients.", "PMID": 975473}
{"id": "pubmed23n0032_10279", "title": "The hemodynamic effects of induced supraventricular tachycardia in man.", "content": "The circulatory effects of supraventricular tachycardia (SVT) were studied in eight patients who reported disabling symptoms during paroxysms of the arrhythmia. Supraventricular tachycardia was induced in each patient by rapid atrial pacing or with atrial premature stimuli. Hemodynamic parameters in sinus rhythm and following the initiation of SVT were recorded and compared. The following mean values were observed in sinus rhythm (SR) and SVT. Heart rate (beats/min): SR 79, SVT 183; P-R interval (msec): during SR, 154; during SVT, 256; ratio of mean P-R intervals to mean R-R cycl lengths: SR 20%, SVT 76%; brachial artery pressures (mmHg): SR 141, SVT 99; cardiac index (L/min/m2): SR 3.6, SVT 2.2; pulmonary artery pressures (mmHg): SR 18/7, SVT 26/15; peak right atrial pressures (mm Hg): SR 4, SVT 17. Large waves appeared in the right atrium during SVT due to atrial contraction against closed tricuspid valves. Pulsus alternans were observed in each case during SVT. Despite the presence of chest pain during SVT, the coronary arteries were normally patent in four patients who underwent coronary arteriography.", "contents": "The hemodynamic effects of induced supraventricular tachycardia in man. The circulatory effects of supraventricular tachycardia (SVT) were studied in eight patients who reported disabling symptoms during paroxysms of the arrhythmia. Supraventricular tachycardia was induced in each patient by rapid atrial pacing or with atrial premature stimuli. Hemodynamic parameters in sinus rhythm and following the initiation of SVT were recorded and compared. The following mean values were observed in sinus rhythm (SR) and SVT. Heart rate (beats/min): SR 79, SVT 183; P-R interval (msec): during SR, 154; during SVT, 256; ratio of mean P-R intervals to mean R-R cycl lengths: SR 20%, SVT 76%; brachial artery pressures (mmHg): SR 141, SVT 99; cardiac index (L/min/m2): SR 3.6, SVT 2.2; pulmonary artery pressures (mmHg): SR 18/7, SVT 26/15; peak right atrial pressures (mm Hg): SR 4, SVT 17. Large waves appeared in the right atrium during SVT due to atrial contraction against closed tricuspid valves. Pulsus alternans were observed in each case during SVT. Despite the presence of chest pain during SVT, the coronary arteries were normally patent in four patients who underwent coronary arteriography.", "PMID": 975474}
{"id": "pubmed23n0032_10280", "title": "Thallium 201 myocardial imaging in patients with pulmonary hypertension.", "content": "The appearance of the right ventricular myocardium on thallium 201 myocardial perfusion images was evaluated in patients with chronic pulmonary hypertension and compared to patients without pulmonary hypertension. Four groups of patients were studied: 1) eight normals, 2) five patients with angiographically documented coronary artery disease and normal pulmonary artery pressures, 3) ten patients with moderate to severe pulmonary parenchymal or vascular disease and documented pulmonary hypertension and 4) eight patients with chronic left ventricular dysfunction and pulmonary hypertension discovered during cardiac catheterization. The right ventricular free wall was visualized on the thallium 201 myocardial perfusion image in only one of eight normals (group 1) and in only one of the five patients with coronary artery disease (group 2) and measured 0.5 cm and 0.9 cm in thickness, respectively. In patients with documented pulmonary hypertension the right ventricle was visualized on low contrast thallium 201 myocardial perfusion image in all patients. The apparent right ventricular free wall thickness measured from the ungated thallium 201 myocardial perfusion images was 1.7 +/- 0.3 cm in group 3 and 1.5 +/- 0.2 cm in group 4. Right ventricular hypertrophy was detected by electrocardiography in only five of ten patients in group 3 and only one of eight patients in group 4. Thallium 201 myocardial perfusion imaging appears to be a useful technique for assessing the effects of chronic pulmonary hypertension on the right ventricular myocardium.", "contents": "Thallium 201 myocardial imaging in patients with pulmonary hypertension. The appearance of the right ventricular myocardium on thallium 201 myocardial perfusion images was evaluated in patients with chronic pulmonary hypertension and compared to patients without pulmonary hypertension. Four groups of patients were studied: 1) eight normals, 2) five patients with angiographically documented coronary artery disease and normal pulmonary artery pressures, 3) ten patients with moderate to severe pulmonary parenchymal or vascular disease and documented pulmonary hypertension and 4) eight patients with chronic left ventricular dysfunction and pulmonary hypertension discovered during cardiac catheterization. The right ventricular free wall was visualized on the thallium 201 myocardial perfusion image in only one of eight normals (group 1) and in only one of the five patients with coronary artery disease (group 2) and measured 0.5 cm and 0.9 cm in thickness, respectively. In patients with documented pulmonary hypertension the right ventricle was visualized on low contrast thallium 201 myocardial perfusion image in all patients. The apparent right ventricular free wall thickness measured from the ungated thallium 201 myocardial perfusion images was 1.7 +/- 0.3 cm in group 3 and 1.5 +/- 0.2 cm in group 4. Right ventricular hypertrophy was detected by electrocardiography in only five of ten patients in group 3 and only one of eight patients in group 4. Thallium 201 myocardial perfusion imaging appears to be a useful technique for assessing the effects of chronic pulmonary hypertension on the right ventricular myocardium.", "PMID": 975475}
{"id": "pubmed23n0032_10281", "title": "Spontaneous resolution of massive congenital tricuspid insufficiency.", "content": "Four cases of congenital tricuspid insufficiency (CTI) are presented in whom spontaneous improvement occured. There was no evidence of Ebstein's disease in three of the four. All presented with similar clinical and laboratory findings including massive cardiomegaly, mild to moderate congestive heart failure, and moderate to severe desaturation. Catheterization data demonstrated severe TI and right-to-left atrial shunting. During the period of follow-up, up to 3.5 years, all symptoms have spontaneously disappeared. Clinical and angiocardiographic evidence for minimal residual TI have remained. Right atrial and right ventricular volume measurements were markedly abnormal initially and returned to normal or near normal. These measurements may be a useful method for quantitating the severity of tricuspid insufficiency. The course of the three infants with massive CTI (without Ebstein's disease) differs from previous reports and may be important both in formulating future management plans and in defining the etiology of the disorder.", "contents": "Spontaneous resolution of massive congenital tricuspid insufficiency. Four cases of congenital tricuspid insufficiency (CTI) are presented in whom spontaneous improvement occured. There was no evidence of Ebstein's disease in three of the four. All presented with similar clinical and laboratory findings including massive cardiomegaly, mild to moderate congestive heart failure, and moderate to severe desaturation. Catheterization data demonstrated severe TI and right-to-left atrial shunting. During the period of follow-up, up to 3.5 years, all symptoms have spontaneously disappeared. Clinical and angiocardiographic evidence for minimal residual TI have remained. Right atrial and right ventricular volume measurements were markedly abnormal initially and returned to normal or near normal. These measurements may be a useful method for quantitating the severity of tricuspid insufficiency. The course of the three infants with massive CTI (without Ebstein's disease) differs from previous reports and may be important both in formulating future management plans and in defining the etiology of the disorder.", "PMID": 975476}
{"id": "pubmed23n0032_10282", "title": "Right ventricular volume characteristics in ventricular septal defect.", "content": "Right and left ventricular volume characteristics were determined from biplane cineangiocardiography in 37 patients with isolated ventricular septal defects. Patients were divided into three categories as determined by the degree of left-to-right shunt: small shunt-less than 35% of pulmonary blood flow (N=9); moderate shunt-35-49% (N=8), and large shunt-greater than 50% (N=20). Right ventricular (RV) end-diastolic volume was increased above normal in 15 of 20 studies performed in patients with large left-to-right shunts and averaged 159 +/- 10% of normal (P less than 0.001). In contrast, only one of the patients in the small shunt group and only half of the patients in the moderate shunt group showed increases in RV end-diastolic volume. The increase in RV volume was proportional to the corresponding increase in left ventricular end-diastolic volume, with the right ventricle ranging from 48 to 116% of LV end-diastolic volume (average 83%). Right ventricular ejection fraction was normal in all patient groups. Right ventricular outpur was increased commensurate with the increases in the RV end-diastolic volume. These data indicate that substantial augmentation in RV end-diastolic volume does occur in patients with isolated ventricular septal defects and large left-to-right shunts. These data can be explained by the significant diastolic and \"isovolumic\" shunting from left ventricle to right ventricle which occurs in these patients.", "contents": "Right ventricular volume characteristics in ventricular septal defect. Right and left ventricular volume characteristics were determined from biplane cineangiocardiography in 37 patients with isolated ventricular septal defects. Patients were divided into three categories as determined by the degree of left-to-right shunt: small shunt-less than 35% of pulmonary blood flow (N=9); moderate shunt-35-49% (N=8), and large shunt-greater than 50% (N=20). Right ventricular (RV) end-diastolic volume was increased above normal in 15 of 20 studies performed in patients with large left-to-right shunts and averaged 159 +/- 10% of normal (P less than 0.001). In contrast, only one of the patients in the small shunt group and only half of the patients in the moderate shunt group showed increases in RV end-diastolic volume. The increase in RV volume was proportional to the corresponding increase in left ventricular end-diastolic volume, with the right ventricle ranging from 48 to 116% of LV end-diastolic volume (average 83%). Right ventricular ejection fraction was normal in all patient groups. Right ventricular outpur was increased commensurate with the increases in the RV end-diastolic volume. These data indicate that substantial augmentation in RV end-diastolic volume does occur in patients with isolated ventricular septal defects and large left-to-right shunts. These data can be explained by the significant diastolic and \"isovolumic\" shunting from left ventricle to right ventricle which occurs in these patients.", "PMID": 975477}
{"id": "pubmed23n0032_10283", "title": "Quantitative analysis of pulmonary vascular disease in complete transposition of the great arteries.", "content": "Forty autopsy cases of complete transposition of the great arteries (TGA) and 22 autopsy cases of ventricular septal defect (VSD) were analyzed histologically for evidence of vascular damage due to pulmonary vascular disease (PVD). Positive correlations were generally observed between an index of pulmonary vascular disease (IPVD) and blood pressure of pulmonary circulation. No significant difference in IPVD was found between TGA and VSD in the first five months of life, when cases of each disease were compared at similar blood pressure levels. After that age, however, IPVD was much higher in TGA, and particularly severe PVD in this disease was demonstrated histologically. Morphometrical analysis of the pulmonary artery revealed hypertrophy of the muscular cost in response to elevated blood pressure. However, the progress of medial hypertrophy was retarded in TGA in the first five months, and medial thickness in arteries of cases of TGA older than five months was only 70% of that in VSD at the same blood pressure levels. Suppression of this process of reinforcement of the arterial wall in response to the stress of high pulmonary pressure was regarded as one of the important factors precipitating severe pulmonary vascular disease in transposition of the great arteries.", "contents": "Quantitative analysis of pulmonary vascular disease in complete transposition of the great arteries. Forty autopsy cases of complete transposition of the great arteries (TGA) and 22 autopsy cases of ventricular septal defect (VSD) were analyzed histologically for evidence of vascular damage due to pulmonary vascular disease (PVD). Positive correlations were generally observed between an index of pulmonary vascular disease (IPVD) and blood pressure of pulmonary circulation. No significant difference in IPVD was found between TGA and VSD in the first five months of life, when cases of each disease were compared at similar blood pressure levels. After that age, however, IPVD was much higher in TGA, and particularly severe PVD in this disease was demonstrated histologically. Morphometrical analysis of the pulmonary artery revealed hypertrophy of the muscular cost in response to elevated blood pressure. However, the progress of medial hypertrophy was retarded in TGA in the first five months, and medial thickness in arteries of cases of TGA older than five months was only 70% of that in VSD at the same blood pressure levels. Suppression of this process of reinforcement of the arterial wall in response to the stress of high pulmonary pressure was regarded as one of the important factors precipitating severe pulmonary vascular disease in transposition of the great arteries.", "PMID": 975478}
{"id": "pubmed23n0032_10284", "title": "Results of a two-year health education campaign on dietary behavior. The Stanford Three Community Study.", "content": "A two-year, bilingual mass-media health education campaign was carried out in two communities with a third serving as control. In one of the two treated communities, personal couseling of high risk individuals (\"intensive instruction\") took place. Dietary behavior, before and after the campaigns, was assessed using a dietary questionnaire which estimated the average daily consumption by participants of cholesterol, saturated fat, and polyunsaturated fat. Both intensive instruction and the mass-media campaigns led to significant reductions (20-40%) in cholesterol and saturated fat consumption in both men and women, with intensively instructed men tending to outperform men exposed to mass-media alone. In general, the improvements in the treated communities were maintained over the two years of the study, whereas the initial small drops in the control community at the end of the first year were followed by recidivism during the second year. Polyunsaturated fat consumption fell slightly in all three communities so that increases in P/S ratios in the two treated communities were of only modest degree. Mean changes in plasma cholesterol concentration for the various cohorts under study were higly correlated with those which would have been predicted on the basis of the self-reported changes in dietary behavior. The results indicate that mass-media health education campaigns can lead to significant changes in dietary practices in the general population with potentially important public health implications.", "contents": "Results of a two-year health education campaign on dietary behavior. The Stanford Three Community Study. A two-year, bilingual mass-media health education campaign was carried out in two communities with a third serving as control. In one of the two treated communities, personal couseling of high risk individuals (\"intensive instruction\") took place. Dietary behavior, before and after the campaigns, was assessed using a dietary questionnaire which estimated the average daily consumption by participants of cholesterol, saturated fat, and polyunsaturated fat. Both intensive instruction and the mass-media campaigns led to significant reductions (20-40%) in cholesterol and saturated fat consumption in both men and women, with intensively instructed men tending to outperform men exposed to mass-media alone. In general, the improvements in the treated communities were maintained over the two years of the study, whereas the initial small drops in the control community at the end of the first year were followed by recidivism during the second year. Polyunsaturated fat consumption fell slightly in all three communities so that increases in P/S ratios in the two treated communities were of only modest degree. Mean changes in plasma cholesterol concentration for the various cohorts under study were higly correlated with those which would have been predicted on the basis of the self-reported changes in dietary behavior. The results indicate that mass-media health education campaigns can lead to significant changes in dietary practices in the general population with potentially important public health implications.", "PMID": 975479}
{"id": "pubmed23n0032_10285", "title": "Bilateral ductal origin of the pulmonary arteries. Systemic-pulmonary arterial anastomosis as first stage in planned total correction.", "content": "Absence of continuity between the heart and the pulmonary arteries has the potential for total correction. When the pulmonary arteries are narrow, a palliative first stage systemic-pulmonary arterial anastomosis serves to enlarge the pulmonary arteries so that, with time, they become of adequate caliber for total correction. In a case with bilateral ductal origin of pulmonary arteries, palliative anastomosis between the aorta and right pulmonary artery was performed as the first stage in a program planned for ultimate total correction.", "contents": "Bilateral ductal origin of the pulmonary arteries. Systemic-pulmonary arterial anastomosis as first stage in planned total correction. Absence of continuity between the heart and the pulmonary arteries has the potential for total correction. When the pulmonary arteries are narrow, a palliative first stage systemic-pulmonary arterial anastomosis serves to enlarge the pulmonary arteries so that, with time, they become of adequate caliber for total correction. In a case with bilateral ductal origin of pulmonary arteries, palliative anastomosis between the aorta and right pulmonary artery was performed as the first stage in a program planned for ultimate total correction.", "PMID": 975480}
{"id": "pubmed23n0032_10286", "title": "Alveolar rhabdomyosarcoma involving the heart. An echocardiographic, angiographic and pathologic study.", "content": "A 12-year-old girl, who had had an above-knee amputation of the left leg for a soft tissue sarcoma six years prior to this admission, presented with syncope, angina, and signs of aortic stenosis. Echocardiography demonstrated a mass arising from the interventricular septum with obstruction of the left ventricular outflow tract and aortic valve. Cardiac catheterization and angiography confirmed these findings. At operation, a tumor was found involving the interventricular septum. Histologically, it was an alveolar rhabdomyosarcoma, and it appeared similar to the previous tumor. The patient was treated by partial surgical resection, cytotoxic drugs and localized radiation therapy and has been followed by serial echocardiography.", "contents": "Alveolar rhabdomyosarcoma involving the heart. An echocardiographic, angiographic and pathologic study. A 12-year-old girl, who had had an above-knee amputation of the left leg for a soft tissue sarcoma six years prior to this admission, presented with syncope, angina, and signs of aortic stenosis. Echocardiography demonstrated a mass arising from the interventricular septum with obstruction of the left ventricular outflow tract and aortic valve. Cardiac catheterization and angiography confirmed these findings. At operation, a tumor was found involving the interventricular septum. Histologically, it was an alveolar rhabdomyosarcoma, and it appeared similar to the previous tumor. The patient was treated by partial surgical resection, cytotoxic drugs and localized radiation therapy and has been followed by serial echocardiography.", "PMID": 975481}
{"id": "pubmed23n0032_10287", "title": "Chronic hypersensitivity lung disease with recurrent episodes of hypersensitivity pneumonitis due to a contaminated central humidifer.", "content": "A child with a 4-year history of acute and chronic respiratory symptoms of unknown aetiology was investigated for hypersensitivity pneumonitis. Lung disease due to inhalation of material from a contaminated central humidifier was suggested by the clinical history, the presence of precipitating antibodies in the serum against the humidifier water, a pulmonary response to challenge with the humidifier water, and marked improvement after removal of the humidifier. No fungi were cultured from the humidifier nor were antibodies against a number of fungal antigens identified by radioimmunoassay inhibition techniques. Antigenic material was found in the humidifier water and the household water prior to its reaching the humidifier. This antigenic material was not found in laboratory tap water supplied from the same general source (Lake Michigan) but from a different pumping station. Three of the child's siblings gave histories suggestive of a single concurrent episode of acute hypersensitivity pneumonitis and one sibling had a history suggestive of chronic hypersensitivity lung disease. No association could be found between HLA-haplotypy and disease in the patient and the siblings.", "contents": "Chronic hypersensitivity lung disease with recurrent episodes of hypersensitivity pneumonitis due to a contaminated central humidifer. A child with a 4-year history of acute and chronic respiratory symptoms of unknown aetiology was investigated for hypersensitivity pneumonitis. Lung disease due to inhalation of material from a contaminated central humidifier was suggested by the clinical history, the presence of precipitating antibodies in the serum against the humidifier water, a pulmonary response to challenge with the humidifier water, and marked improvement after removal of the humidifier. No fungi were cultured from the humidifier nor were antibodies against a number of fungal antigens identified by radioimmunoassay inhibition techniques. Antigenic material was found in the humidifier water and the household water prior to its reaching the humidifier. This antigenic material was not found in laboratory tap water supplied from the same general source (Lake Michigan) but from a different pumping station. Three of the child's siblings gave histories suggestive of a single concurrent episode of acute hypersensitivity pneumonitis and one sibling had a history suggestive of chronic hypersensitivity lung disease. No association could be found between HLA-haplotypy and disease in the patient and the siblings.", "PMID": 975488}
{"id": "pubmed23n0032_10288", "title": "The effect of a new anti-allergic drug ICI 74, 9I7, given by aerosol, on nasal stenosis induced by allergen.", "content": "Nasal airways resistance was measured in ten patients with allergic rhinitis during intranasal application of an extract of grass pollen. Pretreatment with placebo did not inhibit the increase in nasal airways resistance, whereas ICI 74,917 administered from a pressurized aerosol gave almost complete protection. ICI 74,917 was well tolerated and no evidence was obtained of local hyposensitization during the period of the study.", "contents": "The effect of a new anti-allergic drug ICI 74, 9I7, given by aerosol, on nasal stenosis induced by allergen. Nasal airways resistance was measured in ten patients with allergic rhinitis during intranasal application of an extract of grass pollen. Pretreatment with placebo did not inhibit the increase in nasal airways resistance, whereas ICI 74,917 administered from a pressurized aerosol gave almost complete protection. ICI 74,917 was well tolerated and no evidence was obtained of local hyposensitization during the period of the study.", "PMID": 975489}
{"id": "pubmed23n0032_10289", "title": "Oral aspirin challenges in asthmatic patients: a study of plasma histamine.", "content": "Under carefully controlled conditions, seven aspirin-intolerant asthmatic patients were challenged with oral aspirin and experienced respiratory tract reactions with a decline in forced expiratory volume in 1 sec (FEV1) ranging from 26 to 64%. Venous blood samples, which were collected during the challenges, showed a rise in plasma histamine in all seven patients. The increase in plasma histamine occurred at the onset of their respiratory reactions and those patients with the most severe asthmatic responses were found to have the highest and most prolonged levels of plasma histamine. The aspirin-intolerant asthmatic patients were able to ingest Maalox or sodium salicylate without untoward effects, decline in FEV1 values or changes in plasma histamine levels. Ten non-asthmatic individuals and eight out of ten asthmatic control patients were able to ingest aspirin without any reactions or changes in their plasma histamine levels. However, two asthmatic control individuals, with severe asthma requiring treatment with moderate dosages of corticosteroids, were found to have elevated pre-challenge plasma histamine levels which increased during their ASA challenges despite the absence of respiratory reactions or changes in FEV1 values. It is possible that these two individuals were unsuspected aspirin-intolerant asthmatics. These studies demonstrate that asthmatic reactions to acetylsalicylates are associated with release of histamine into plasma in the subgroup of asthmatic patients with the aspirin-intolerance syndrome. Such a finding suggests that histamine may be one of the mediators of bronchospasm in aspirin-induced asthma.", "contents": "Oral aspirin challenges in asthmatic patients: a study of plasma histamine. Under carefully controlled conditions, seven aspirin-intolerant asthmatic patients were challenged with oral aspirin and experienced respiratory tract reactions with a decline in forced expiratory volume in 1 sec (FEV1) ranging from 26 to 64%. Venous blood samples, which were collected during the challenges, showed a rise in plasma histamine in all seven patients. The increase in plasma histamine occurred at the onset of their respiratory reactions and those patients with the most severe asthmatic responses were found to have the highest and most prolonged levels of plasma histamine. The aspirin-intolerant asthmatic patients were able to ingest Maalox or sodium salicylate without untoward effects, decline in FEV1 values or changes in plasma histamine levels. Ten non-asthmatic individuals and eight out of ten asthmatic control patients were able to ingest aspirin without any reactions or changes in their plasma histamine levels. However, two asthmatic control individuals, with severe asthma requiring treatment with moderate dosages of corticosteroids, were found to have elevated pre-challenge plasma histamine levels which increased during their ASA challenges despite the absence of respiratory reactions or changes in FEV1 values. It is possible that these two individuals were unsuspected aspirin-intolerant asthmatics. These studies demonstrate that asthmatic reactions to acetylsalicylates are associated with release of histamine into plasma in the subgroup of asthmatic patients with the aspirin-intolerance syndrome. Such a finding suggests that histamine may be one of the mediators of bronchospasm in aspirin-induced asthma.", "PMID": 975490}
{"id": "pubmed23n0032_10290", "title": "Value of lymphoblast transformation test in cow's milk protein intestinal intolerance.", "content": "Lymphoblast transformation tests were carried out in the presence of alpha-lactalbumin and beta-lactoglobulin. In patients with cow's milk protein intestinal intolerance in seventeen of forty-five (37.8%) the lymphoblast transformation tests were positive. Sensitization in the first month of life seemed to favour lymphoblast transformation. In control children in only four of forty-three (9.5%) the lymphoblast transformation tests were positive, the difference from intolerant patients being significant 0.01 less than P less than 0.001. Lymphoblast transformation tests were negative in the seven children with active coeliac disease. Although a negative test does not exclude cow's milk protein intolerance, lymphoblast transformation tests can be considered a useful aid in diagnosis because of its specificity.", "contents": "Value of lymphoblast transformation test in cow's milk protein intestinal intolerance. Lymphoblast transformation tests were carried out in the presence of alpha-lactalbumin and beta-lactoglobulin. In patients with cow's milk protein intestinal intolerance in seventeen of forty-five (37.8%) the lymphoblast transformation tests were positive. Sensitization in the first month of life seemed to favour lymphoblast transformation. In control children in only four of forty-three (9.5%) the lymphoblast transformation tests were positive, the difference from intolerant patients being significant 0.01 less than P less than 0.001. Lymphoblast transformation tests were negative in the seven children with active coeliac disease. Although a negative test does not exclude cow's milk protein intolerance, lymphoblast transformation tests can be considered a useful aid in diagnosis because of its specificity.", "PMID": 975491}
{"id": "pubmed23n0032_10291", "title": "An application of non-linear regression analysis to tests of liver function.", "content": "Serum samples from 42 cases of acute hepatitis were analysed for up to 10 tests of liver function. The results of these tests were then analysed by standard statistical methods. A curve fitting exercise was performed and regression coefficients and comparative data were calculated for each of six curve types, relating to each pair of liver function tests. The correlation coefficients found were then tested for significance. From the 216 correlation coefficients tested eleven were found to be highly significant (p less than 0.001) and of these, seven were of non-linear regression curves. Examples have been given of the use of regression equations in assessing the relative sensitivities of different tests at levels on the borderline of the normal range.", "contents": "An application of non-linear regression analysis to tests of liver function. Serum samples from 42 cases of acute hepatitis were analysed for up to 10 tests of liver function. The results of these tests were then analysed by standard statistical methods. A curve fitting exercise was performed and regression coefficients and comparative data were calculated for each of six curve types, relating to each pair of liver function tests. The correlation coefficients found were then tested for significance. From the 216 correlation coefficients tested eleven were found to be highly significant (p less than 0.001) and of these, seven were of non-linear regression curves. Examples have been given of the use of regression equations in assessing the relative sensitivities of different tests at levels on the borderline of the normal range.", "PMID": 975492}
{"id": "pubmed23n0032_10292", "title": "Elevation of serum creatine kinase in severe hypokalemic hyperaldosteronism.", "content": "The association between hypokalemia and increased serum creatine kinase has been investigated. Two patients were found who had severe hypokalemic hyperaldosteronism who had elevation of serum creatine kinase activity. The CPK activity returned to normal values after fluid and potassium replacement. In a prospective study of 129 patients with hypokalemia which did not include any patients with hyperaldosteronism, 12 were found to have increased CPK activity but each of these had some other severe disease process which could account for the increased CPK. Three other patients with K+ (2.2 MEq/1 did not have increased CPK. It is concluded that patients with severe hypokalemia such as that occurring in hyperaldosteronism with muscle weakness may have elevated CPK directly related to their hypokalemia. In other patients with hypokalemia, 10% may have increased CPK but this is related to other disease processes.", "contents": "Elevation of serum creatine kinase in severe hypokalemic hyperaldosteronism. The association between hypokalemia and increased serum creatine kinase has been investigated. Two patients were found who had severe hypokalemic hyperaldosteronism who had elevation of serum creatine kinase activity. The CPK activity returned to normal values after fluid and potassium replacement. In a prospective study of 129 patients with hypokalemia which did not include any patients with hyperaldosteronism, 12 were found to have increased CPK activity but each of these had some other severe disease process which could account for the increased CPK. Three other patients with K+ (2.2 MEq/1 did not have increased CPK. It is concluded that patients with severe hypokalemia such as that occurring in hyperaldosteronism with muscle weakness may have elevated CPK directly related to their hypokalemia. In other patients with hypokalemia, 10% may have increased CPK but this is related to other disease processes.", "PMID": 975493}
{"id": "pubmed23n0032_10293", "title": "Plasma and urinary aldosterone measurement in healthy subjects with a radioimmunoassay kit not requiring chromatography.", "content": "We examined the application of a radioimmunoassay kit, not requiring chromatography, to the measurement of aldosterone in healthy human subjects. Plasma aldosterone, urinary aldosterone, and plasma renin activity measurements were performed on samples from 47 subjects under conditions in which dietary sodium intake, posture, and times of sample collection were rigidly controlled. Nomograms are presented to illustrate the continuous, rather well defined, inverse relationship between 24 h sodium excretion and aldosterone measurements in plasma and urine. Mean plasma and urinary aldosterone values for subjects on normal sodium diets were 0.332+/-0.211 nmol/liter and 36.6+/-18.6 nmol/24 h and agreed well with previously reported values. A significant (p less than 0.05 increase in 24 h urinary aldosterone, plasma aldosterone and plasma renin activity resulted with decreased dietary sodium intake and conversely, a significant (p less than 0.05) decrease in each hormone occurred with increased dietary sodium intake. Rising from the supine to upright position resulted in a 230% increase in plasma aldosterone concentrations and a 284% increase in plasma renin activity.", "contents": "Plasma and urinary aldosterone measurement in healthy subjects with a radioimmunoassay kit not requiring chromatography. We examined the application of a radioimmunoassay kit, not requiring chromatography, to the measurement of aldosterone in healthy human subjects. Plasma aldosterone, urinary aldosterone, and plasma renin activity measurements were performed on samples from 47 subjects under conditions in which dietary sodium intake, posture, and times of sample collection were rigidly controlled. Nomograms are presented to illustrate the continuous, rather well defined, inverse relationship between 24 h sodium excretion and aldosterone measurements in plasma and urine. Mean plasma and urinary aldosterone values for subjects on normal sodium diets were 0.332+/-0.211 nmol/liter and 36.6+/-18.6 nmol/24 h and agreed well with previously reported values. A significant (p less than 0.05 increase in 24 h urinary aldosterone, plasma aldosterone and plasma renin activity resulted with decreased dietary sodium intake and conversely, a significant (p less than 0.05) decrease in each hormone occurred with increased dietary sodium intake. Rising from the supine to upright position resulted in a 230% increase in plasma aldosterone concentrations and a 284% increase in plasma renin activity.", "PMID": 975494}
{"id": "pubmed23n0032_10294", "title": "Estimation of amitriptyline and its metabolites in serum and urine by GLC using nitrogen-specific detector.", "content": "1. A gas-chromatographic procedure for the estimation of amitriptyline and its metabolites in serum and urine using a nitrogen-specific detector is described. Specially cleaned glassware and purified solvents are used for the extraction of serum to further minimize extraneous peaks. Trimethylamine is added to serum before extraction to improve the recovery of drugs. Urine is refluxed at pH approximately 1 to hydrolyze the conjugates and to convert hydroxymetabolites to corresponding dehydro compounds. Serum is not hydrolyzed. 2. Two internal standards, one a tertiary amine similar in structure to amitriptyline and the other a secondary amine similar in structure to nortriptyline, are added to the specimen prior to extraction to obviate the need for accurate measurements of volumes during extraction and analysis. Urine and serum are washed with organic solvents at acidic pH to remove neutral and acidic impurities. Secondary bases are converted to their acetyl derivatives. 3. In the serum of a patient who is on amitriptyline therapy or who has ingested an overdose of amitriptyline, nortriptyline, a pharmacologically active metabolite is also measured. However, detection or estimation of hydroxymetabolites in serum is not clinically relevant. Hydroxylation index of an individual patient is determined by measuring the ratio of nortriptyline to its hydroxymetabolite in urine. 4. Amitriptyline and nortriptyline can be estimated in serum at a lower level of 10 and 20 ng/ml respectively. The procedure is linear over a wide range of amitriptyline and its metabolites. The use of an electronic integrator allows the estimation of different compounds with 100 fold difference in their concentration from the same chromatogram.", "contents": "Estimation of amitriptyline and its metabolites in serum and urine by GLC using nitrogen-specific detector. 1. A gas-chromatographic procedure for the estimation of amitriptyline and its metabolites in serum and urine using a nitrogen-specific detector is described. Specially cleaned glassware and purified solvents are used for the extraction of serum to further minimize extraneous peaks. Trimethylamine is added to serum before extraction to improve the recovery of drugs. Urine is refluxed at pH approximately 1 to hydrolyze the conjugates and to convert hydroxymetabolites to corresponding dehydro compounds. Serum is not hydrolyzed. 2. Two internal standards, one a tertiary amine similar in structure to amitriptyline and the other a secondary amine similar in structure to nortriptyline, are added to the specimen prior to extraction to obviate the need for accurate measurements of volumes during extraction and analysis. Urine and serum are washed with organic solvents at acidic pH to remove neutral and acidic impurities. Secondary bases are converted to their acetyl derivatives. 3. In the serum of a patient who is on amitriptyline therapy or who has ingested an overdose of amitriptyline, nortriptyline, a pharmacologically active metabolite is also measured. However, detection or estimation of hydroxymetabolites in serum is not clinically relevant. Hydroxylation index of an individual patient is determined by measuring the ratio of nortriptyline to its hydroxymetabolite in urine. 4. Amitriptyline and nortriptyline can be estimated in serum at a lower level of 10 and 20 ng/ml respectively. The procedure is linear over a wide range of amitriptyline and its metabolites. The use of an electronic integrator allows the estimation of different compounds with 100 fold difference in their concentration from the same chromatogram.", "PMID": 975495}
{"id": "pubmed23n0032_10295", "title": "Iterpretation of clinical chemical data with the aid of automatic data processing.", "content": "I summarize conventional applications of computers in clinical laboratories and discuss the possible role of the computer in providing assistance in interpretation of laboratory data through the use of data bases. The data bases include effects of drugs on the results of laboratory tests, factors influencing references values, and conversion factors for converting data from conventional units to SIunits. Reported diagnostic applications of the computer are discussed with reference to both clinically oriented and laboratory oriented programs.", "contents": "Iterpretation of clinical chemical data with the aid of automatic data processing. I summarize conventional applications of computers in clinical laboratories and discuss the possible role of the computer in providing assistance in interpretation of laboratory data through the use of data bases. The data bases include effects of drugs on the results of laboratory tests, factors influencing references values, and conversion factors for converting data from conventional units to SIunits. Reported diagnostic applications of the computer are discussed with reference to both clinically oriented and laboratory oriented programs.", "PMID": 975497}
{"id": "pubmed23n0032_10296", "title": "Determination of phenprocoumon, an anticoagulant, in human plasma.", "content": "Phenprocoumon is extracted from acidified plasma, the organic phase evaporated, and part of the residue, in ethanol, is quantitatively applied to a thin-layer plate. After separation, the quantity of phenprocoumon is assayed by fluorescence densitometry in situ. Results are reproducible to about 2.5%. The lower limit of detection is 0.1 mg/liter, which makes the method fully applicable to human plasma, because therapeutic concentrations range from 1 to 3 mg/liter. Seven determinations can be made within 3 h. For toxicological purposes, a qualitative analysis can be done in a shorter time, because the phenprocoumon spots are visible under ultraviolet light at 254 nm.", "contents": "Determination of phenprocoumon, an anticoagulant, in human plasma. Phenprocoumon is extracted from acidified plasma, the organic phase evaporated, and part of the residue, in ethanol, is quantitatively applied to a thin-layer plate. After separation, the quantity of phenprocoumon is assayed by fluorescence densitometry in situ. Results are reproducible to about 2.5%. The lower limit of detection is 0.1 mg/liter, which makes the method fully applicable to human plasma, because therapeutic concentrations range from 1 to 3 mg/liter. Seven determinations can be made within 3 h. For toxicological purposes, a qualitative analysis can be done in a shorter time, because the phenprocoumon spots are visible under ultraviolet light at 254 nm.", "PMID": 975498}
{"id": "pubmed23n0032_10297", "title": "Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous-flow analysis.", "content": "Idescribe an assay for total cholesterol in serum, with use of the AutoAnalyzer ii (Technicon), in which cholesterol esters are saponified by alkali, cholesterol is held in aqueous micellar solution with a surfactant (Triton X-100) and oxidized by cholesterol oxidase, and the hydrogen peroxide produced is measured by chelation with Ti4+ and xylenol orange. An assay for free cholesterol in serum, based on similar principles, is also described, and the two can be run simultaneously on a dual-channel AutoAnalyzer II. Standard solutions of cholesterol in isopropanol have poorer carryover characteristics than sera, and therefore do not reach the same continuous-flow steady state as sera of equivalent concentrations. Consequent potential calibration errors are avoided by using micellar solutions of cholesterol containing albumin for standardization. The formation of cholesterol peroxide in solutions of cholesterol in isopropanol is demonstrated, and this constitutes another potential source of error in the calibration of enzymic cholesterol assays. In analyzing patients' sera, results of the total cholesterol assay correlate well with those of a mechanized method in which cholesterol esterase and cholesterol oxidase are used; an automated Abell method, calibrated with solutions of cholesterol in isopropanol, gave slightly higher values. Determinations of the ratio of free cholesterol to total cholesterol by our automated cholesterol exidase assays given values that agree well with published results in which digitonin precipitation is used.", "contents": "Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous-flow analysis. Idescribe an assay for total cholesterol in serum, with use of the AutoAnalyzer ii (Technicon), in which cholesterol esters are saponified by alkali, cholesterol is held in aqueous micellar solution with a surfactant (Triton X-100) and oxidized by cholesterol oxidase, and the hydrogen peroxide produced is measured by chelation with Ti4+ and xylenol orange. An assay for free cholesterol in serum, based on similar principles, is also described, and the two can be run simultaneously on a dual-channel AutoAnalyzer II. Standard solutions of cholesterol in isopropanol have poorer carryover characteristics than sera, and therefore do not reach the same continuous-flow steady state as sera of equivalent concentrations. Consequent potential calibration errors are avoided by using micellar solutions of cholesterol containing albumin for standardization. The formation of cholesterol peroxide in solutions of cholesterol in isopropanol is demonstrated, and this constitutes another potential source of error in the calibration of enzymic cholesterol assays. In analyzing patients' sera, results of the total cholesterol assay correlate well with those of a mechanized method in which cholesterol esterase and cholesterol oxidase are used; an automated Abell method, calibrated with solutions of cholesterol in isopropanol, gave slightly higher values. Determinations of the ratio of free cholesterol to total cholesterol by our automated cholesterol exidase assays given values that agree well with published results in which digitonin precipitation is used.", "PMID": 975499}
{"id": "pubmed23n0032_10298", "title": "Microcalorimetric determination of glucose in reference samples of serum.", "content": "Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two methods. A discussion of the basis of the microcalorimetric measurements is presented.", "contents": "Microcalorimetric determination of glucose in reference samples of serum. Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two methods. A discussion of the basis of the microcalorimetric measurements is presented.", "PMID": 975500}
{"id": "pubmed23n0032_10299", "title": "Automated method for L-carnitine determination.", "content": "Because of renewed interest in a possible connection between carnitine, lipid disorders, and myopathy, an automated method of analysis is desirable. Deproteinization of serum by use of membrane filter cones and automated assay with a bichromatic analyzer (the ABA-100) substantially increases efficiency without sacrificing the specificity and accuracy of the original manual enzymatic method. The described procedure allows for analysis of 80 speciments a day and is thus suitable for screening of selected populations. Normal values found in blood sera of adults were in the range of 25.0-73.8 mu mol/liter and the method has sufficient sensitivity to accurately measure concentrations as small as 10 mu mol/liter.", "contents": "Automated method for L-carnitine determination. Because of renewed interest in a possible connection between carnitine, lipid disorders, and myopathy, an automated method of analysis is desirable. Deproteinization of serum by use of membrane filter cones and automated assay with a bichromatic analyzer (the ABA-100) substantially increases efficiency without sacrificing the specificity and accuracy of the original manual enzymatic method. The described procedure allows for analysis of 80 speciments a day and is thus suitable for screening of selected populations. Normal values found in blood sera of adults were in the range of 25.0-73.8 mu mol/liter and the method has sufficient sensitivity to accurately measure concentrations as small as 10 mu mol/liter.", "PMID": 975501}
{"id": "pubmed23n0032_10300", "title": "Determination of serum retinol (vitamin A) by high-speed liquid chromatography.", "content": "We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].", "contents": "Determination of serum retinol (vitamin A) by high-speed liquid chromatography. We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].", "PMID": 975502}
{"id": "pubmed23n0032_10301", "title": "Automated method for determination of mercury in urine.", "content": "I describe a sensitive, specific, automated method for determination of mercury in urine by the \"flameless atomic absorption\" technique. One can analyze 20 samples per hour, with less than 0.5-ml samples of urine. The detection limit is 0.01 mumol/liter (2mug/liter) and the mean coefficient of variation 12%. The procedure is being used to monitor mercury excretion by workers exposed to mercury.", "contents": "Automated method for determination of mercury in urine. I describe a sensitive, specific, automated method for determination of mercury in urine by the \"flameless atomic absorption\" technique. One can analyze 20 samples per hour, with less than 0.5-ml samples of urine. The detection limit is 0.01 mumol/liter (2mug/liter) and the mean coefficient of variation 12%. The procedure is being used to monitor mercury excretion by workers exposed to mercury.", "PMID": 975503}
{"id": "pubmed23n0032_10302", "title": "Amino acid composition of human nail, as measured by gas-liquid chromatography.", "content": "We describe a quantitative method for determination of histidine and 16 other amino acids in a nail hydrolysate. This is based on the formation of trifluoroacetyl amino acid methyl esters and gas-liquid chromatographic analysis with use of two columns of mixed stationary phases. Histidine is determined as a triple derivative Nalpha-trifluroacetyl-Nim-carbethoxy methyl histidinate. The specificity and reproducibility of the method are illustrated. As little as 1 mg of amino acid per gram of nail can be determined, and 15 hydrolysates can be completely analyzed in 10 working days. Data obtained from multiple analyses of 10 nail hydrolysates by the method described closely parallel those obtained in another laboratory, in which an amino acid analyzer was used.", "contents": "Amino acid composition of human nail, as measured by gas-liquid chromatography. We describe a quantitative method for determination of histidine and 16 other amino acids in a nail hydrolysate. This is based on the formation of trifluoroacetyl amino acid methyl esters and gas-liquid chromatographic analysis with use of two columns of mixed stationary phases. Histidine is determined as a triple derivative Nalpha-trifluroacetyl-Nim-carbethoxy methyl histidinate. The specificity and reproducibility of the method are illustrated. As little as 1 mg of amino acid per gram of nail can be determined, and 15 hydrolysates can be completely analyzed in 10 working days. Data obtained from multiple analyses of 10 nail hydrolysates by the method described closely parallel those obtained in another laboratory, in which an amino acid analyzer was used.", "PMID": 975504}
{"id": "pubmed23n0032_10303", "title": "Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer.", "content": "A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.", "contents": "Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer. A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.", "PMID": 975505}
{"id": "pubmed23n0032_10304", "title": "Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.", "content": "We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.", "contents": "Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes. We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.", "PMID": 975506}
{"id": "pubmed23n0032_10305", "title": "Cholesterol oxidase-based determination, by continuous-flow analysis, of total and free cholesterol in serum.", "content": "We describe a continuous-flow, automated determination of total cholesterol in serum, which is based on enzymatic hydrolysis of cholesterol esters, oxidation of cholesterol by cholesterol oxidase, and colorimetric measurement of liberated perioxide with 4-aminoantipyrine, phenol, and peroxidase. Free cholesterol is determined with the same AutoAnalyzer II manifold and reagents, except that cholesterol esterase is omitted from the reagent. Cholesterol-in-serum materials that have been assayed by an established method are used for calibration. We found this approach to be necessary because primary cholesterol standards in organic solvents are incompatible with the aqueous reagent. Results of the enzymatic total cholesterol method correlated well with those by an AutoAnalyzer II method which involves an extraction with isopropanol and the Liebermann-Burchard color reaction (total cholesterol, g/liter, yenz= 0991xlb +0.05;r=0.996). Results of the enzymatic free cholesterol procedure agreed satisfactorily with one in which free cholesterol is precipitated as the digitonide and subsequently analyzed colorimetrically with the Liebermann-Burchard reaction (free cholesterol, %, yenz = 0.982xdig -0.7;r= 0.956).", "contents": "Cholesterol oxidase-based determination, by continuous-flow analysis, of total and free cholesterol in serum. We describe a continuous-flow, automated determination of total cholesterol in serum, which is based on enzymatic hydrolysis of cholesterol esters, oxidation of cholesterol by cholesterol oxidase, and colorimetric measurement of liberated perioxide with 4-aminoantipyrine, phenol, and peroxidase. Free cholesterol is determined with the same AutoAnalyzer II manifold and reagents, except that cholesterol esterase is omitted from the reagent. Cholesterol-in-serum materials that have been assayed by an established method are used for calibration. We found this approach to be necessary because primary cholesterol standards in organic solvents are incompatible with the aqueous reagent. Results of the enzymatic total cholesterol method correlated well with those by an AutoAnalyzer II method which involves an extraction with isopropanol and the Liebermann-Burchard color reaction (total cholesterol, g/liter, yenz= 0991xlb +0.05;r=0.996). Results of the enzymatic free cholesterol procedure agreed satisfactorily with one in which free cholesterol is precipitated as the digitonide and subsequently analyzed colorimetrically with the Liebermann-Burchard reaction (free cholesterol, %, yenz = 0.982xdig -0.7;r= 0.956).", "PMID": 975508}
{"id": "pubmed23n0032_10306", "title": "Kinetic determination of hexosaminidases for Tay-Sachs heterozygote screening.", "content": "We describe a method for continuous kinetic measurement of hexosaminidase activity, and have applied it to detection of heterozygotes for Tay-Sachs gene. In contrast to existing single-point methods, a ph of 4.5, which is optimal for hexosaminidase activity on the substrate (4-methylumbelliferyl-N-acetyl-beta-d-glucosaminide) is maintained while the increase in fluorescence produced by 4-methylumbelliferone, the reaction product, is being determined. Under the conditions described the reaction follows zero-order kinetics, and activity is linearly related to serum dilution. There is a fairly narrow but practicable range of optimal substrate concentration. The presence of substrate in concentrations exceeding 1 mmol/liter results in progressive quenching of fluorescence and a decrease in apparent enzyme activity. The procedure is standardized with 4-methylumbelliferone in a solution of the substrate. This method is adaptable to use with automated multiple-point discrete-sample devices and especially with the fluorometric kinetic analyzers now being developed.", "contents": "Kinetic determination of hexosaminidases for Tay-Sachs heterozygote screening. We describe a method for continuous kinetic measurement of hexosaminidase activity, and have applied it to detection of heterozygotes for Tay-Sachs gene. In contrast to existing single-point methods, a ph of 4.5, which is optimal for hexosaminidase activity on the substrate (4-methylumbelliferyl-N-acetyl-beta-d-glucosaminide) is maintained while the increase in fluorescence produced by 4-methylumbelliferone, the reaction product, is being determined. Under the conditions described the reaction follows zero-order kinetics, and activity is linearly related to serum dilution. There is a fairly narrow but practicable range of optimal substrate concentration. The presence of substrate in concentrations exceeding 1 mmol/liter results in progressive quenching of fluorescence and a decrease in apparent enzyme activity. The procedure is standardized with 4-methylumbelliferone in a solution of the substrate. This method is adaptable to use with automated multiple-point discrete-sample devices and especially with the fluorometric kinetic analyzers now being developed.", "PMID": 975509}
{"id": "pubmed23n0032_10307", "title": "Gas-chromatographic analysis for 1,2:5,6-dianhydrogalactitol, an anti-neoplastic agent, in plasma.", "content": "We describe a gas chromatographic method for measuring submicrogram quantities of dianhydrogalactitol, a promising antineoplastic agent (currently undergoing clinical trials in humans), in plasma. The drug is first extracted from blood plasma by saturating the aqueous phase with solid potassium carbonate and extracting with isopropanol/chloroform (9/1 by vol). It is then converted to the corresponding n-butaneboronic ester by reaction at room temperature with butaneboronic acid and chromatographed on an SE-30 (3%) column, with flame ionization detection. Practicality of the method for monitoring drug distribution was demonstrated by administering dianhydrogalactitol in therapeutic doses to a dog and monitoring its concentrations in blood for the next 2 h.", "contents": "Gas-chromatographic analysis for 1,2:5,6-dianhydrogalactitol, an anti-neoplastic agent, in plasma. We describe a gas chromatographic method for measuring submicrogram quantities of dianhydrogalactitol, a promising antineoplastic agent (currently undergoing clinical trials in humans), in plasma. The drug is first extracted from blood plasma by saturating the aqueous phase with solid potassium carbonate and extracting with isopropanol/chloroform (9/1 by vol). It is then converted to the corresponding n-butaneboronic ester by reaction at room temperature with butaneboronic acid and chromatographed on an SE-30 (3%) column, with flame ionization detection. Practicality of the method for monitoring drug distribution was demonstrated by administering dianhydrogalactitol in therapeutic doses to a dog and monitoring its concentrations in blood for the next 2 h.", "PMID": 975510}
{"id": "pubmed23n0032_10308", "title": "Clinical usefulness of measuring prothrombin time as a routine admission test.", "content": "A questionnaire indicating the presence of a history or physical findings consistent with liver desease or bleeding disorders was completed by house officers on 301 admissions to a Veterans Administration medical service. Each patient had prothrombin time determined on admission. Only two of 107 patients for whom the prothrombin time was measured as a screening test had an abnormal results and one of these was normal when repeated. Of 73 patients with a history of alcoholism but no other pertient case history or physical examination results, only one had an abnormal prothrombin time. Of the remaining 121 patients, who had a pertinent history or physical examination, 41 had an abnormal admission prothrombin time. We conclude that measurement of prothrombin time adds little additional information to that obtained by history and physical examination in screening for liver disease and coagulation defects unless the patient has specific clinical evidence of liver disease, anticoagulation, or other conditions predisposing to bleeding disorders.", "contents": "Clinical usefulness of measuring prothrombin time as a routine admission test. A questionnaire indicating the presence of a history or physical findings consistent with liver desease or bleeding disorders was completed by house officers on 301 admissions to a Veterans Administration medical service. Each patient had prothrombin time determined on admission. Only two of 107 patients for whom the prothrombin time was measured as a screening test had an abnormal results and one of these was normal when repeated. Of 73 patients with a history of alcoholism but no other pertient case history or physical examination results, only one had an abnormal prothrombin time. Of the remaining 121 patients, who had a pertinent history or physical examination, 41 had an abnormal admission prothrombin time. We conclude that measurement of prothrombin time adds little additional information to that obtained by history and physical examination in screening for liver disease and coagulation defects unless the patient has specific clinical evidence of liver disease, anticoagulation, or other conditions predisposing to bleeding disorders.", "PMID": 975511}
{"id": "pubmed23n0032_10309", "title": "A faster radioimmunoassay of progesterone in plasma, with use of polyethylene glycol as precipitant.", "content": "We report a reliable radioimmunoassay for plasma progesterone in which polyethylene glycol (\"Carbowax 6000\") is used to separate antibody-bound and free hormone. Initially, progesterone is concentrated by putting the sample through a column of LH-20 Sephadex. Procedural losses in each sample are not monitored. We compared the reliability and practicality of this method with that of another method in which procedural losses are monitored for each sample; accuracy and precision were similar. Our method saves technical assay time and scintillation counter space and time without sacrificing reliability.", "contents": "A faster radioimmunoassay of progesterone in plasma, with use of polyethylene glycol as precipitant. We report a reliable radioimmunoassay for plasma progesterone in which polyethylene glycol (\"Carbowax 6000\") is used to separate antibody-bound and free hormone. Initially, progesterone is concentrated by putting the sample through a column of LH-20 Sephadex. Procedural losses in each sample are not monitored. We compared the reliability and practicality of this method with that of another method in which procedural losses are monitored for each sample; accuracy and precision were similar. Our method saves technical assay time and scintillation counter space and time without sacrificing reliability.", "PMID": 975512}
{"id": "pubmed23n0032_10310", "title": "High-pressure liquid-chromatographic determination of 5-(4-hydroxyphenyl)-5-phenylhydantoin in humanurine.", "content": "We describe a sensitive and precise high-pressure liquid-chromatographic method for measurement of total 5-(4-hydroxyphenyl)-5-phenylhydantoin, a metabolite of phenytoin, in urine. An aliquot of urine, containing 5-(4-methylphenyl)-5-phenylhydantoin as an internal standard, is processed and chromatographed. The metabolite and internal standard are identified from their retention times and quantitated from their relative response factors. The metabolite is separated from normal urine constituents and internal standard in less than 8 min. The sensitivity of the method is such that after the usual dose it can be measured in 0.5 ml of urine; the lower limit of detection is 300 ng.", "contents": "High-pressure liquid-chromatographic determination of 5-(4-hydroxyphenyl)-5-phenylhydantoin in humanurine. We describe a sensitive and precise high-pressure liquid-chromatographic method for measurement of total 5-(4-hydroxyphenyl)-5-phenylhydantoin, a metabolite of phenytoin, in urine. An aliquot of urine, containing 5-(4-methylphenyl)-5-phenylhydantoin as an internal standard, is processed and chromatographed. The metabolite and internal standard are identified from their retention times and quantitated from their relative response factors. The metabolite is separated from normal urine constituents and internal standard in less than 8 min. The sensitivity of the method is such that after the usual dose it can be measured in 0.5 ml of urine; the lower limit of detection is 300 ng.", "PMID": 975513}
{"id": "pubmed23n0032_10311", "title": "Proficiency testing for blood-gas quality control.", "content": "We have conducted a voluntary, community blood-gas proficiency testing program, with use of tonometered human blood, for 32 analyzers located in 16 laboratories. Instruments initially showed inaccuracies as large as -30.8 to +17.3% for po(2), and -14.0 to +42.9% for pco(2), but inaccuracy and imprecision decreased in most laboratories during the program. For a typical 15-week period, mean group precision (CV) was 4.3 To 5.1% for po(2) from 6.92 to 33.3 Pa (52 to 250 mmHg), and 4.0 to 6.9% for pco(2) from 2.0 to 6.8 Pa (15 to 51 mmHg). This program can detect increasing imprecision or inaccuracy caused by analyzer deterioration, and can identify interlaboratory or interinstrument bias and problems not detected by participant quality-control programs. Participants have used the proficiency information in discussing data quality with clinicians, promoting internal control and maintenance programs, and justifying instrument purchases. We believe that proficiency-testing documentation of variability in blood-gas analysis may help to establish realistic patient-care protocols.", "contents": "Proficiency testing for blood-gas quality control. We have conducted a voluntary, community blood-gas proficiency testing program, with use of tonometered human blood, for 32 analyzers located in 16 laboratories. Instruments initially showed inaccuracies as large as -30.8 to +17.3% for po(2), and -14.0 to +42.9% for pco(2), but inaccuracy and imprecision decreased in most laboratories during the program. For a typical 15-week period, mean group precision (CV) was 4.3 To 5.1% for po(2) from 6.92 to 33.3 Pa (52 to 250 mmHg), and 4.0 to 6.9% for pco(2) from 2.0 to 6.8 Pa (15 to 51 mmHg). This program can detect increasing imprecision or inaccuracy caused by analyzer deterioration, and can identify interlaboratory or interinstrument bias and problems not detected by participant quality-control programs. Participants have used the proficiency information in discussing data quality with clinicians, promoting internal control and maintenance programs, and justifying instrument purchases. We believe that proficiency-testing documentation of variability in blood-gas analysis may help to establish realistic patient-care protocols.", "PMID": 975514}
{"id": "pubmed23n0032_10312", "title": "Results for serum cholesterol and triglycerides by gas-liquid chromatography, as compared with a continuous-flow technique.", "content": "We assessed the usefulness of gas-liquid chromatography forthe routine and reference measurement of cholesterol and triglycerides (triacylglycerols). Compared with results obtained by an AutoAnalyzer II method, correlation was good with both serum specimens (triglycerides, r = 0.88; cholesterol, r = 0.90) and lipoprotein fractions isolated by ultracentrifugation (triglycerides, r = 0.98; cholesterol, r = 0.98). However, none of the comparisons was completely free of analytical bias, and in general the AutoAnalyzer method underestimated the cholesterol value and overestimated triglycerides. The obvious advantage of the AutoAnalyzer is its greater analytical speed, but the chromatographic method appeared to be more accurate and precise and to suffer less from interfering substances, and in addition gave information about the cholesterol ester and triglyceride composition. We expect gas-liquid chromatography to be of major importance in the calibration of other analytical methods.", "contents": "Results for serum cholesterol and triglycerides by gas-liquid chromatography, as compared with a continuous-flow technique. We assessed the usefulness of gas-liquid chromatography forthe routine and reference measurement of cholesterol and triglycerides (triacylglycerols). Compared with results obtained by an AutoAnalyzer II method, correlation was good with both serum specimens (triglycerides, r = 0.88; cholesterol, r = 0.90) and lipoprotein fractions isolated by ultracentrifugation (triglycerides, r = 0.98; cholesterol, r = 0.98). However, none of the comparisons was completely free of analytical bias, and in general the AutoAnalyzer method underestimated the cholesterol value and overestimated triglycerides. The obvious advantage of the AutoAnalyzer is its greater analytical speed, but the chromatographic method appeared to be more accurate and precise and to suffer less from interfering substances, and in addition gave information about the cholesterol ester and triglyceride composition. We expect gas-liquid chromatography to be of major importance in the calibration of other analytical methods.", "PMID": 975515}
{"id": "pubmed23n0032_10313", "title": "Gas-chromatographic analysis for therapeutic concentration of imipramine and disipramine in plasma, with use of a nitrogen detector.", "content": "We describe a method for the simultaneous gas-chromatographic analysis of imipramine and desipramine (the active N-desmethyl metabolite) at therapeutic concentrations in human plasma, with use of a nitrogen detector. Imipramine is measured as the unchanged base and desipramine as its N-tribluoroacetyl derivative. Promazine is the internal standard. The lower limit of sensitivity is 5 mug/liter for each drug, and the within-day coefficients of variation for the determination of imipramine and desipramine, respectively, are 6.0% and 3.3%. We measured concentrations of these drugs both in patients receiving therapeutic doses of imipramine and in those who had ingested an overdose.", "contents": "Gas-chromatographic analysis for therapeutic concentration of imipramine and disipramine in plasma, with use of a nitrogen detector. We describe a method for the simultaneous gas-chromatographic analysis of imipramine and desipramine (the active N-desmethyl metabolite) at therapeutic concentrations in human plasma, with use of a nitrogen detector. Imipramine is measured as the unchanged base and desipramine as its N-tribluoroacetyl derivative. Promazine is the internal standard. The lower limit of sensitivity is 5 mug/liter for each drug, and the within-day coefficients of variation for the determination of imipramine and desipramine, respectively, are 6.0% and 3.3%. We measured concentrations of these drugs both in patients receiving therapeutic doses of imipramine and in those who had ingested an overdose.", "PMID": 975516}
{"id": "pubmed23n0032_10314", "title": "Enzymatic, fluorometric assay of alpha-amylase in serum.", "content": "An enzymatic, fluorometric method for kinetic assay of serum alpha-amylase (1,4alpha-D-glucan 4-glucanohydrolase, EC 3.2.1.1) is described. A soluble starch is used as substrate, in tris(hydroxymethyl)methylamine buffer. All measurements are made in Pyrex cuvettes at 37 degrees C, with a reaction volume of 1.16 ml. The assay is based on the following reaction sequence: (see article) The rate of appearance of fluorescence of NADH (lambdaex = 365 nm, lambdaem = 460 nm), developed in the indicator reaction (4), is measured and equated to the activity of alpha-amylase in serum. A calibration plot of the change of fluorescence per min vs. enzyme concentration shows a good proportionality in the range of 0.50-5.0 kU/liter.", "contents": "Enzymatic, fluorometric assay of alpha-amylase in serum. An enzymatic, fluorometric method for kinetic assay of serum alpha-amylase (1,4alpha-D-glucan 4-glucanohydrolase, EC 3.2.1.1) is described. A soluble starch is used as substrate, in tris(hydroxymethyl)methylamine buffer. All measurements are made in Pyrex cuvettes at 37 degrees C, with a reaction volume of 1.16 ml. The assay is based on the following reaction sequence: (see article) The rate of appearance of fluorescence of NADH (lambdaex = 365 nm, lambdaem = 460 nm), developed in the indicator reaction (4), is measured and equated to the activity of alpha-amylase in serum. A calibration plot of the change of fluorescence per min vs. enzyme concentration shows a good proportionality in the range of 0.50-5.0 kU/liter.", "PMID": 975517}
{"id": "pubmed23n0032_10315", "title": "Comparison of results by three kit methods for assay of serum gastrin.", "content": "We evaluated and compared Nuclear International, Schwarz/Mann, and Squibb kit methods for measurement of serum gastrin with respect to intra-run precision, analytical recovery of added gastrin, and values obtained for healthy fasting adults and for selected patients. As a result of this study, the Schwarz/Mann kit was chosen for use in our laboratory.", "contents": "Comparison of results by three kit methods for assay of serum gastrin. We evaluated and compared Nuclear International, Schwarz/Mann, and Squibb kit methods for measurement of serum gastrin with respect to intra-run precision, analytical recovery of added gastrin, and values obtained for healthy fasting adults and for selected patients. As a result of this study, the Schwarz/Mann kit was chosen for use in our laboratory.", "PMID": 975518}
{"id": "pubmed23n0032_10316", "title": "Modified determination of total and inorganic mercury in urine by cold vapor atomic absorption sectrometry.", "content": "In this procedure a single-beam spectrophotometer is used without background correction. By the method of Magos [Analyst (London) 96, 847 (1971)] mercury in undigested urine is complexed to L-cysteine in acid solution. At high pH and in the presence of stannous ions, mercury ions are reduced to elemental mercury. The mercury vapor is partitioned above the reagent solution in a specially designed chemical reduction apparatus similar in principle to that used by Kubasik et al. [Clin. Chem. 18, 1326 (1972)]. The vapor is then flushed by air through an \"absorption\" cell, where the absorption of the mercury line at 253.7 nm is measured. The value obtained for inorganic mercury subtracted from that for total mercury gives a value for organic mercury. CV's for the inorganic mercury procedure at 40 and 5 mug/liter concentrations were 3.1% and 7.5%, respectively. The detection limit is 0.82 mug/liter. The CV for the total-mercury procedure (20 mug/liter) was 2.6%. Mean analytical recoveries of organic and inorganic mercury were 96.5% and 101%, respectively. We investigated storage conditions for urine and compared results by the present technique with those by activation analysis. Our method is a convenient way to screen individuals who have been exposed to a mercury hazard.", "contents": "Modified determination of total and inorganic mercury in urine by cold vapor atomic absorption sectrometry. In this procedure a single-beam spectrophotometer is used without background correction. By the method of Magos [Analyst (London) 96, 847 (1971)] mercury in undigested urine is complexed to L-cysteine in acid solution. At high pH and in the presence of stannous ions, mercury ions are reduced to elemental mercury. The mercury vapor is partitioned above the reagent solution in a specially designed chemical reduction apparatus similar in principle to that used by Kubasik et al. [Clin. Chem. 18, 1326 (1972)]. The vapor is then flushed by air through an \"absorption\" cell, where the absorption of the mercury line at 253.7 nm is measured. The value obtained for inorganic mercury subtracted from that for total mercury gives a value for organic mercury. CV's for the inorganic mercury procedure at 40 and 5 mug/liter concentrations were 3.1% and 7.5%, respectively. The detection limit is 0.82 mug/liter. The CV for the total-mercury procedure (20 mug/liter) was 2.6%. Mean analytical recoveries of organic and inorganic mercury were 96.5% and 101%, respectively. We investigated storage conditions for urine and compared results by the present technique with those by activation analysis. Our method is a convenient way to screen individuals who have been exposed to a mercury hazard.", "PMID": 975519}
{"id": "pubmed23n0032_10317", "title": "Use of values for calcium and protein in serum, and of a derived index obtained from a probability population sample.", "content": "We measured serum protein and calcium concentrations in 2340 individuals between 10 and 96 years of age from 900 families chosen by probability methods to give a representative population. These values were used to calculate an index, based on a regression analysis of serum protein on calcium, which was then treated as a new variable. Age-sex specific reference values and frequency distributions are presented for this index as well as for protein and calcium calculated by both parametric and nonparametric methods.", "contents": "Use of values for calcium and protein in serum, and of a derived index obtained from a probability population sample. We measured serum protein and calcium concentrations in 2340 individuals between 10 and 96 years of age from 900 families chosen by probability methods to give a representative population. These values were used to calculate an index, based on a regression analysis of serum protein on calcium, which was then treated as a new variable. Age-sex specific reference values and frequency distributions are presented for this index as well as for protein and calcium calculated by both parametric and nonparametric methods.", "PMID": 975520}
{"id": "pubmed23n0032_10318", "title": "Interference by acetaminophen in the glucose oxidase-peroxidase method for blood glucose determination.", "content": "Acetaminophen, p-aminophenol, and oxyphenbutazone interfere with the glucose oxidase/peroxidase method for glucose. Structurally related compounds that lack a free phenolic hydroxyl group (acetanilide, aniline, and phenylbutazone) do not interfere. During the analytical procedure acetaminophen is consumed. One mole of acetaminophen leads to an apparent loss of four moles of glucose. The hexokinase/glucose-6-phosphate dehydrogenase method (Boehringer Hexokinase method) is not affected by these substances.", "contents": "Interference by acetaminophen in the glucose oxidase-peroxidase method for blood glucose determination. Acetaminophen, p-aminophenol, and oxyphenbutazone interfere with the glucose oxidase/peroxidase method for glucose. Structurally related compounds that lack a free phenolic hydroxyl group (acetanilide, aniline, and phenylbutazone) do not interfere. During the analytical procedure acetaminophen is consumed. One mole of acetaminophen leads to an apparent loss of four moles of glucose. The hexokinase/glucose-6-phosphate dehydrogenase method (Boehringer Hexokinase method) is not affected by these substances.", "PMID": 975521}
{"id": "pubmed23n0032_10319", "title": "Reduced variation of tracer binding in digoxin radioimmunoassay by use of (125I)-labeled tyrosine-methyl-ester derivative: relation of thyroxine concentration to binding.", "content": "Between-sample variation in tracer binding in the 125I-labeled digoxin radioimmunoassay was investigated with two tracers, 3-O-succinyl-digoxigenin-[125I]-labeled tyrosine and [125I]-labeled tyrosine-methyl-ester-digoxin. Digoxin-free serum samples having various concentrations of thyroxine were assayed with both tracers. The percentage of tracer bound when the samples were assayed with the first-mentioned tracer was increased significantly for the low thyroxine groups when compared to the normal (P less than 0.001) or the high thyroxine groups (P less than 0.05). Little difference existed when the latter tracer was used. There was variation in tracer binding when serum from dogs dosed with thyrotropin was assayed with the first tracer, but there was little variation with the second. Tracer binding may be influenced by thyroxine-binding proteins. Variation in tracer binding appears to be reduced when [125I]-labeled tyrosine-methyl-ester-digoxin is used.", "contents": "Reduced variation of tracer binding in digoxin radioimmunoassay by use of (125I)-labeled tyrosine-methyl-ester derivative: relation of thyroxine concentration to binding. Between-sample variation in tracer binding in the 125I-labeled digoxin radioimmunoassay was investigated with two tracers, 3-O-succinyl-digoxigenin-[125I]-labeled tyrosine and [125I]-labeled tyrosine-methyl-ester-digoxin. Digoxin-free serum samples having various concentrations of thyroxine were assayed with both tracers. The percentage of tracer bound when the samples were assayed with the first-mentioned tracer was increased significantly for the low thyroxine groups when compared to the normal (P less than 0.001) or the high thyroxine groups (P less than 0.05). Little difference existed when the latter tracer was used. There was variation in tracer binding when serum from dogs dosed with thyrotropin was assayed with the first tracer, but there was little variation with the second. Tracer binding may be influenced by thyroxine-binding proteins. Variation in tracer binding appears to be reduced when [125I]-labeled tyrosine-methyl-ester-digoxin is used.", "PMID": 975522}
{"id": "pubmed23n0032_10320", "title": "Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer.", "content": "Thymol blue monophosphate is evaluated as a substrate for use in kinetic bichromatic analysis of serum alkaline phosphatase activity. The effect of abnormally high concentrations of hemoglobin, bilirubin, or chylomicrons is negligible. The expected range for ambulatory adults established by assaying sera from 206 blood donors, was 12-44 U/lliter at 37 degrees C. Comparison of the method with a commonly used p-nitrophenyl phosphate method yielded a correlation coefficient, r, of 0.9949. Advantages of the method are the reduced cost per test and the exceptionally stable substrate.", "contents": "Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer. Thymol blue monophosphate is evaluated as a substrate for use in kinetic bichromatic analysis of serum alkaline phosphatase activity. The effect of abnormally high concentrations of hemoglobin, bilirubin, or chylomicrons is negligible. The expected range for ambulatory adults established by assaying sera from 206 blood donors, was 12-44 U/lliter at 37 degrees C. Comparison of the method with a commonly used p-nitrophenyl phosphate method yielded a correlation coefficient, r, of 0.9949. Advantages of the method are the reduced cost per test and the exceptionally stable substrate.", "PMID": 975523}
{"id": "pubmed23n0032_10321", "title": "Simple modification of a liquid scintillation counter to decrease cost of and time for radioimmunoassay.", "content": "A simple, inexpensive modification in the elevator mechanism of a liquid scintillation counter allows for radioimmunoassay tubes (12 X 75 mm) containing the antibody-bound radioligand to be counted directly, thereby obviating the need for counting vials and considerably decreasing the amount of fluor used. Furthermore, because assay and counting are all done in the same tube, the modification shortens assay time and increases precision by avoiding what is often the most time consuming and inaccurate step, the transfer of antibody-bound radioligand to a counting vial. We find that use of this modification results in about a 75% decrease in the overall cost for materials and a substantial decrease in assay time.", "contents": "Simple modification of a liquid scintillation counter to decrease cost of and time for radioimmunoassay. A simple, inexpensive modification in the elevator mechanism of a liquid scintillation counter allows for radioimmunoassay tubes (12 X 75 mm) containing the antibody-bound radioligand to be counted directly, thereby obviating the need for counting vials and considerably decreasing the amount of fluor used. Furthermore, because assay and counting are all done in the same tube, the modification shortens assay time and increases precision by avoiding what is often the most time consuming and inaccurate step, the transfer of antibody-bound radioligand to a counting vial. We find that use of this modification results in about a 75% decrease in the overall cost for materials and a substantial decrease in assay time.", "PMID": 975527}
{"id": "pubmed23n0032_10322", "title": "Toward absolute methods in clinical chemistry: application of mass fragmentography to high-accuracy analyses.", "content": "We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be determined, the relative standard deviation of these methods, based on duplicate determinations, is between 1.3 and 2.7%. The very high specificity of the methods, in combination with the fact that the ratio between labeled and unlabeled molecules is determined with a high degree of accuracy, makes it likely that the most significant errors in the methods are related to errors in pipetting. The possibility is discussed that these methods might be developed into \"absolute\" or \"definitive\" methods by subjecting each individual step in the determination to a detailed analysis with respect to possible error. We also discuss some of the consequences of the different reference methods on the choice of routine methods.", "contents": "Toward absolute methods in clinical chemistry: application of mass fragmentography to high-accuracy analyses. We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be determined, the relative standard deviation of these methods, based on duplicate determinations, is between 1.3 and 2.7%. The very high specificity of the methods, in combination with the fact that the ratio between labeled and unlabeled molecules is determined with a high degree of accuracy, makes it likely that the most significant errors in the methods are related to errors in pipetting. The possibility is discussed that these methods might be developed into \"absolute\" or \"definitive\" methods by subjecting each individual step in the determination to a detailed analysis with respect to possible error. We also discuss some of the consequences of the different reference methods on the choice of routine methods.", "PMID": 975540}
{"id": "pubmed23n0032_10323", "title": "Spectrophotometric and electrochemical determinations of L(+)-lactate in blood by use of lactate dehydrogenase from yeast.", "content": "Lactate can be determined rapidly in blood by spectrophotometric and amperometric (enzyme electrode) procedures based on its oxidation by ferricyanide, the reaction being catalyzed with yeast L(+)-lactate dehydrogenase (cytochrome b2) (EC 1.1.2.3). In the photometric method lactate can be measured in a few minutes, but blood samples must first be deproteinized. In the amperometric procedure no treatment of blood is needed except ferricyanide addition. The enzyme electrode we used has a response time shorter than 1 min when its critical variables are optimized. Preliminary standardization is reduced to minimum operation, because electrode response is proportional to lactate concentration over a wide range (0.1 to 8.0 mol/liter) and many determinations can be done with little cost in enzyme. A simple electrical device (\"two-electrode device\") is described that is well suited for furture micro-cell construction. Lactate determinations on a series of normal blood samples show no deviation between results by these new methods and the usual ultraviolet spectrophotometric lactate tests.", "contents": "Spectrophotometric and electrochemical determinations of L(+)-lactate in blood by use of lactate dehydrogenase from yeast. Lactate can be determined rapidly in blood by spectrophotometric and amperometric (enzyme electrode) procedures based on its oxidation by ferricyanide, the reaction being catalyzed with yeast L(+)-lactate dehydrogenase (cytochrome b2) (EC 1.1.2.3). In the photometric method lactate can be measured in a few minutes, but blood samples must first be deproteinized. In the amperometric procedure no treatment of blood is needed except ferricyanide addition. The enzyme electrode we used has a response time shorter than 1 min when its critical variables are optimized. Preliminary standardization is reduced to minimum operation, because electrode response is proportional to lactate concentration over a wide range (0.1 to 8.0 mol/liter) and many determinations can be done with little cost in enzyme. A simple electrical device (\"two-electrode device\") is described that is well suited for furture micro-cell construction. Lactate determinations on a series of normal blood samples show no deviation between results by these new methods and the usual ultraviolet spectrophotometric lactate tests.", "PMID": 975541}
{"id": "pubmed23n0032_10324", "title": "Erythrocyte uroporphyrinogen I synthase activity in diagnosis of acute intermittent porphyria.", "content": "Measurement of the activity of uroporphyrinogen I synthase provides an excellent laboratory aid in the diagnosis of acute intermittent porphyria, particularly in those patients who are asymptomatic or in whom the disease is not biochemically manifested by porphyrin precursor excretion. We describe here a simplified fluorometric method for measuring the activity of this enzyme in whole blood. The assay is based upon a coupled-enzyme procedure in which added delta-aminolevulinic acid and the dehydratase that is present in erythrocytes are used to generate porphobilinogen as substrate for uroporphyrinogen synthase. After appropriate incubation the protein is removed we sensitivity, specificity, and precision of the assay compare well with previously described procedures. Activity in nonporphyric male subjects was 31 (SD, 6.0) nmol of porphyrin formed per milliliter of erythrocytes per hour at 37 degrees C. Application of the method for identifying gene carriers of acute intermittent porphyria is demonstrated in three generations of an affected family.", "contents": "Erythrocyte uroporphyrinogen I synthase activity in diagnosis of acute intermittent porphyria. Measurement of the activity of uroporphyrinogen I synthase provides an excellent laboratory aid in the diagnosis of acute intermittent porphyria, particularly in those patients who are asymptomatic or in whom the disease is not biochemically manifested by porphyrin precursor excretion. We describe here a simplified fluorometric method for measuring the activity of this enzyme in whole blood. The assay is based upon a coupled-enzyme procedure in which added delta-aminolevulinic acid and the dehydratase that is present in erythrocytes are used to generate porphobilinogen as substrate for uroporphyrinogen synthase. After appropriate incubation the protein is removed we sensitivity, specificity, and precision of the assay compare well with previously described procedures. Activity in nonporphyric male subjects was 31 (SD, 6.0) nmol of porphyrin formed per milliliter of erythrocytes per hour at 37 degrees C. Application of the method for identifying gene carriers of acute intermittent porphyria is demonstrated in three generations of an affected family.", "PMID": 975542}
{"id": "pubmed23n0032_10325", "title": "Laboratory confirmation of the diagnosis of cystic fibrosis.", "content": "The recent commercial introduction of a method for detecting albumin in meconium makes screening for cystic fibrosis feasible for many hospitals. If the tests is adopted, confirmatory tests should be available. Quantitative analyses of sweat for sodium by flame photometry and for chloride by silver titration and ion-sleective electrodes are now used as confirmatory tests. We compare results of these confirmatory methods applied to presons with cystic fibrosis, respiratory disorders, or digestive disorders, and to control subjects.", "contents": "Laboratory confirmation of the diagnosis of cystic fibrosis. The recent commercial introduction of a method for detecting albumin in meconium makes screening for cystic fibrosis feasible for many hospitals. If the tests is adopted, confirmatory tests should be available. Quantitative analyses of sweat for sodium by flame photometry and for chloride by silver titration and ion-sleective electrodes are now used as confirmatory tests. We compare results of these confirmatory methods applied to presons with cystic fibrosis, respiratory disorders, or digestive disorders, and to control subjects.", "PMID": 975543}
{"id": "pubmed23n0032_10326", "title": "Radioimmunoassay for serum cortisol with 125I-labeled ligand: comparison of three methods.", "content": "We evaluated a previously modified double-antibody radioimmunoassay for serum cortisol. It was compared with the conventional double-antibody method that includes the usual extraction step, and also with an antibody-coated tube method. In this modified method, cortisol was released from its binding globulin by enzymatic degradation rather than by extraction with ether, and a preincubated mixture of first and second antibody was used to separate antibody-bound cortisol from free. These two steps shortened total asssay time significantly. Results still correlated well (r = 0.87) with results by the conventional melthod, but the antibody-coated tube method gave lower results (r = 0.61). Because of its good correlation with the conventional method, this method was thought to be more accurate. In 52 normal subjects, mean cortisol concentrations at 0800 and 1700 hours were 161 +/- 52 (SD) mug/liter and 91 +/- 27 mug/liter, respectively. In 16 normal subjects, cortisol values before and after dexamethasone treatment (1 mg at midnight) were 134 +/- 53 mug/liter and less than 20 mug/liter. In the same subjects, cortisol concentrations before and 30 min and 60 min after Cortrosyn (synthetic corticotropin1-24, 0.25 mg) administration were 103 +/- 25, 205 +/- 45 and 223 +/- 51 mug/liter, respectively.", "contents": "Radioimmunoassay for serum cortisol with 125I-labeled ligand: comparison of three methods. We evaluated a previously modified double-antibody radioimmunoassay for serum cortisol. It was compared with the conventional double-antibody method that includes the usual extraction step, and also with an antibody-coated tube method. In this modified method, cortisol was released from its binding globulin by enzymatic degradation rather than by extraction with ether, and a preincubated mixture of first and second antibody was used to separate antibody-bound cortisol from free. These two steps shortened total asssay time significantly. Results still correlated well (r = 0.87) with results by the conventional melthod, but the antibody-coated tube method gave lower results (r = 0.61). Because of its good correlation with the conventional method, this method was thought to be more accurate. In 52 normal subjects, mean cortisol concentrations at 0800 and 1700 hours were 161 +/- 52 (SD) mug/liter and 91 +/- 27 mug/liter, respectively. In 16 normal subjects, cortisol values before and after dexamethasone treatment (1 mg at midnight) were 134 +/- 53 mug/liter and less than 20 mug/liter. In the same subjects, cortisol concentrations before and 30 min and 60 min after Cortrosyn (synthetic corticotropin1-24, 0.25 mg) administration were 103 +/- 25, 205 +/- 45 and 223 +/- 51 mug/liter, respectively.", "PMID": 975544}
{"id": "pubmed23n0032_10327", "title": "An 125I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured.", "content": "We report an iodine-125 radioimmunoassay for cortisol in biological fluids, in which interfering binding proteins are enzymatically denatured. An antiserum to cortisol-3-carboxymethyloxime-bovine serum albumin, extremely low cross-reacting with other corticosteroids, was raised in rabbits. A cortisol-3-carboxymethyloxime tyrosine methyl ester derivative was synthesized and labeled with iodine-125 by standard radioiodination techniques. To eliminate the need for extraction and recovery procedures, we digested interfering binding witha proteolytic enzyme, which then was heat-inactivated before adding the labeled derivative and the premixed, preincubated antiserum complex. There was quantitative analytical recovery of esogenous cortisol added to sera from a normal man, a normal woman, and a pregnant woman. Values for the same samples agreed after extraction and chromatographic purification and agreed well with values obtained by other techniques by independent reference laboratories. The five-step assay can be done in 6 h or less.", "contents": "An 125I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured. We report an iodine-125 radioimmunoassay for cortisol in biological fluids, in which interfering binding proteins are enzymatically denatured. An antiserum to cortisol-3-carboxymethyloxime-bovine serum albumin, extremely low cross-reacting with other corticosteroids, was raised in rabbits. A cortisol-3-carboxymethyloxime tyrosine methyl ester derivative was synthesized and labeled with iodine-125 by standard radioiodination techniques. To eliminate the need for extraction and recovery procedures, we digested interfering binding witha proteolytic enzyme, which then was heat-inactivated before adding the labeled derivative and the premixed, preincubated antiserum complex. There was quantitative analytical recovery of esogenous cortisol added to sera from a normal man, a normal woman, and a pregnant woman. Values for the same samples agreed after extraction and chromatographic purification and agreed well with values obtained by other techniques by independent reference laboratories. The five-step assay can be done in 6 h or less.", "PMID": 975545}
{"id": "pubmed23n0032_10328", "title": "Correlation of selected serum constitutents: 2. Consistency of intra-individual correlation values, means, and variances during four months.", "content": "We examined whether inter-individual differences in correlation coefficients previously found among subjects truly reflect consistent inter-individual differences or are time-related within an individual. The consitutents studied in this investigation were (a) the enzmes aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase; and (b) the non=protein nitrogen-containing constituents urea, uric acid, and creatinine. Ten healthy women were each subjected to 15 venipunctures over a five-week period (Series I), and, after a two-month interval, were again subjected to 15 venipunctures over a second five-week period (Series II). Before statistical analysis, the data were corrected for the batch-to-batch (day-to-day) arnalytical variation. There was a signiificant (P less than .05) change in the covariance structure (variances or correlation coefficients, or both) between the two series in four of the 10 subjects for the combination of enzymes, and in three other subjects for the combination of nonprotein nitrogen constitutents. Although we found a significant (P lees than .05) average intra-individual variation in the mean values from series to series in the cases of the three enzymes and urea, the magnitude of the inter-series variation in means was relatively small. CV's were: alkaline phosphatase, 3.4%; lactate dehydrogenase, 2.3+; aspartate aminotransferase, 3.3%; urea, 5.0%; uric acid, 1.0%; and creatinine, 1.2%.", "contents": "Correlation of selected serum constitutents: 2. Consistency of intra-individual correlation values, means, and variances during four months. We examined whether inter-individual differences in correlation coefficients previously found among subjects truly reflect consistent inter-individual differences or are time-related within an individual. The consitutents studied in this investigation were (a) the enzmes aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase; and (b) the non=protein nitrogen-containing constituents urea, uric acid, and creatinine. Ten healthy women were each subjected to 15 venipunctures over a five-week period (Series I), and, after a two-month interval, were again subjected to 15 venipunctures over a second five-week period (Series II). Before statistical analysis, the data were corrected for the batch-to-batch (day-to-day) arnalytical variation. There was a signiificant (P less than .05) change in the covariance structure (variances or correlation coefficients, or both) between the two series in four of the 10 subjects for the combination of enzymes, and in three other subjects for the combination of nonprotein nitrogen constitutents. Although we found a significant (P lees than .05) average intra-individual variation in the mean values from series to series in the cases of the three enzymes and urea, the magnitude of the inter-series variation in means was relatively small. CV's were: alkaline phosphatase, 3.4%; lactate dehydrogenase, 2.3+; aspartate aminotransferase, 3.3%; urea, 5.0%; uric acid, 1.0%; and creatinine, 1.2%.", "PMID": 975546}
{"id": "pubmed23n0032_10329", "title": "An evaluation of the Beckman chloride/carbon dioxide analyzer.", "content": "We compared the Beckman Cl/CO2 Analyzer to the American Instrument Company chloride titrator for measurement of chloride in serum, sweat, and spinal fluid. We also compared it to the Natelson gasometer and a modified continuous-flow (AutoAnalyzer) technique for measurement of carbon dioxide in serum. Effects of bilirubin and hemoglobin on results for both the CO2, and Cl were negligible. The linearity, stability, and precision of the equipment was studied. We believe the Beckman Cl/CO2 Analyzer to be ideal for simultaneous measurement of chloride and carbon dioxide in a pediatric or emergency-determination setting.", "contents": "An evaluation of the Beckman chloride/carbon dioxide analyzer. We compared the Beckman Cl/CO2 Analyzer to the American Instrument Company chloride titrator for measurement of chloride in serum, sweat, and spinal fluid. We also compared it to the Natelson gasometer and a modified continuous-flow (AutoAnalyzer) technique for measurement of carbon dioxide in serum. Effects of bilirubin and hemoglobin on results for both the CO2, and Cl were negligible. The linearity, stability, and precision of the equipment was studied. We believe the Beckman Cl/CO2 Analyzer to be ideal for simultaneous measurement of chloride and carbon dioxide in a pediatric or emergency-determination setting.", "PMID": 975547}
{"id": "pubmed23n0032_10330", "title": "Aspartate aminotransferase activity in human serum. Factors to be considered in supplementation with pyridoxal 5'-phosphate in vitro.", "content": "The pyridoxal phosphate reactivation of the apo form of aspartate aminotransferase (EC 2.6.1.1) in human serum has been studied with \"normal\" and above-normal activity of this enzyme. The extent of the reactionation did not depend on the presence of the substrates, L-aspartate or 2-oxoglutarate. Reactivation was greatest with 110 mumol of added pyridoxal phsophate present per liter during a preinucation for 7 min in tris(hydroxymethyl)methylamine buffer wit;h serum volume fractions ranging from 0.017 to 0.267. In comparison with measurements prformed with no exogenous pyridoxal phosphate present, we found two potential sources of error when this cofactor was added: (a) reagent and sample blanks in the pyridoxal phosphate-supplemented system were two- to eightfold higher and (b) progress curves were nonlinear when L-aspartate rather than 2-oxoglutarate was used as the startin substrate. Aspartate aminotransferase measurement sith pyridoxal phosphate supplementation was slightly more precise than without.", "contents": "Aspartate aminotransferase activity in human serum. Factors to be considered in supplementation with pyridoxal 5'-phosphate in vitro. The pyridoxal phosphate reactivation of the apo form of aspartate aminotransferase (EC 2.6.1.1) in human serum has been studied with \"normal\" and above-normal activity of this enzyme. The extent of the reactionation did not depend on the presence of the substrates, L-aspartate or 2-oxoglutarate. Reactivation was greatest with 110 mumol of added pyridoxal phsophate present per liter during a preinucation for 7 min in tris(hydroxymethyl)methylamine buffer wit;h serum volume fractions ranging from 0.017 to 0.267. In comparison with measurements prformed with no exogenous pyridoxal phosphate present, we found two potential sources of error when this cofactor was added: (a) reagent and sample blanks in the pyridoxal phosphate-supplemented system were two- to eightfold higher and (b) progress curves were nonlinear when L-aspartate rather than 2-oxoglutarate was used as the startin substrate. Aspartate aminotransferase measurement sith pyridoxal phosphate supplementation was slightly more precise than without.", "PMID": 975548}
{"id": "pubmed23n0032_10331", "title": "Serum ionic fluoride: normal range and relationship to age and sex.", "content": "We used the Orion fluoride electrode system to determine the normal range of serum ionic fluoride concentrations and to investigate its relationship to sex and age (A). 87 normal men, aged 18-92 years (mean, 46 years), and 49 normal women, age 19-64 years (mean, 38 years), participated in the study. At the 95% confidence limits, males less than 45 years old had a normal range of 0.29 to 1.52 mumol/litre and males greater than or equal to 45 years old 0.29 + 0.0101 (A-45) to 1.52 + 0.0101 (A-45) mumol/litre. Females, however, had a normal range of 0.022A - 0.32 to 0.022A + 1.07 mumol/litre. A group of 51 men 18-44 years old was compared with a group of 36 men 46-92 years old. The mean serum F- of the older group was shown to be significantly greater (P less than 0.01) than that of the younger group. Factors related to serum ionic fluoride values are (a) tea as an important source of dietary F-, (b) the lack of significant variation during daytime hours, and (c) the lack of significant difference in concentration between serum and plsma F-.", "contents": "Serum ionic fluoride: normal range and relationship to age and sex. We used the Orion fluoride electrode system to determine the normal range of serum ionic fluoride concentrations and to investigate its relationship to sex and age (A). 87 normal men, aged 18-92 years (mean, 46 years), and 49 normal women, age 19-64 years (mean, 38 years), participated in the study. At the 95% confidence limits, males less than 45 years old had a normal range of 0.29 to 1.52 mumol/litre and males greater than or equal to 45 years old 0.29 + 0.0101 (A-45) to 1.52 + 0.0101 (A-45) mumol/litre. Females, however, had a normal range of 0.022A - 0.32 to 0.022A + 1.07 mumol/litre. A group of 51 men 18-44 years old was compared with a group of 36 men 46-92 years old. The mean serum F- of the older group was shown to be significantly greater (P less than 0.01) than that of the younger group. Factors related to serum ionic fluoride values are (a) tea as an important source of dietary F-, (b) the lack of significant variation during daytime hours, and (c) the lack of significant difference in concentration between serum and plsma F-.", "PMID": 975549}
{"id": "pubmed23n0032_10332", "title": "Low apparent creatine kinase activity and prolonged lag phases in serum of patients with metastatic disease: elimination by treatment of sera with sulfhydryl agents.", "content": "We find lag phases exceeding 20 min in measuring creatine kinase activity, by using the kinetic creatin phosphate leads to creatine assay, in sera of some patients with carcinoma metastatic to the liver. Such long lag phases are accompanied by a decreased apparent enzyme activity. These problems are eliminated by adding sulfhydryl agents to the serum before assay, but not by adding more of such agents to the assay reagent. beta-Mercaptoethanol is supperior to Cleland's reagents, glutathione, and cysteine. The long lag phases could not be explained by inadequate activity of the coupling enzymes, interference with the coupling steps, high proportions of cardiacisoenzymes activity, simple oxidation of the enzyme, low concentrations of albumin, or increased concentrations of glutathione reductase, lactate dehydrogenase, or uric acid. We conclude that the prolonged lag phases reflect inadequate reactivation of the enzyme by sulfhydryl agents under the usual assay conditions. Reactivation before assay can prevent potentially serious negative errors in the assay of creatine kinase.", "contents": "Low apparent creatine kinase activity and prolonged lag phases in serum of patients with metastatic disease: elimination by treatment of sera with sulfhydryl agents. We find lag phases exceeding 20 min in measuring creatine kinase activity, by using the kinetic creatin phosphate leads to creatine assay, in sera of some patients with carcinoma metastatic to the liver. Such long lag phases are accompanied by a decreased apparent enzyme activity. These problems are eliminated by adding sulfhydryl agents to the serum before assay, but not by adding more of such agents to the assay reagent. beta-Mercaptoethanol is supperior to Cleland's reagents, glutathione, and cysteine. The long lag phases could not be explained by inadequate activity of the coupling enzymes, interference with the coupling steps, high proportions of cardiacisoenzymes activity, simple oxidation of the enzyme, low concentrations of albumin, or increased concentrations of glutathione reductase, lactate dehydrogenase, or uric acid. We conclude that the prolonged lag phases reflect inadequate reactivation of the enzyme by sulfhydryl agents under the usual assay conditions. Reactivation before assay can prevent potentially serious negative errors in the assay of creatine kinase.", "PMID": 975550}
{"id": "pubmed23n0032_10333", "title": "Evaluation of three commercially available menual \"kit\" methods for triglycerides in serum.", "content": "We compared three commercially available kits for use in determining triglycerides (triacylglycerols) in serum, by different methods, for accuracy and precision, with use of pooled sera of low, normal, and high triglyceride concentrations. Accuracy varied from -17% to +16% of the independently determined value, depending on the method and triglyceride concentration. In general, the Calbiochem method was the most nearly accurate, and only it met Center for Disease Control criteria for precision at all three triglyceride concentrations. The relationships of mean values to the suggested normal range for each method varied, some significant differences being found. Our data demonstrate the importance of a correlation between the independently determined value and the normal range for the method used and stresses the need for normal-range values to accompany all patients' reports.", "contents": "Evaluation of three commercially available menual \"kit\" methods for triglycerides in serum. We compared three commercially available kits for use in determining triglycerides (triacylglycerols) in serum, by different methods, for accuracy and precision, with use of pooled sera of low, normal, and high triglyceride concentrations. Accuracy varied from -17% to +16% of the independently determined value, depending on the method and triglyceride concentration. In general, the Calbiochem method was the most nearly accurate, and only it met Center for Disease Control criteria for precision at all three triglyceride concentrations. The relationships of mean values to the suggested normal range for each method varied, some significant differences being found. Our data demonstrate the importance of a correlation between the independently determined value and the normal range for the method used and stresses the need for normal-range values to accompany all patients' reports.", "PMID": 975551}
{"id": "pubmed23n0032_10334", "title": "More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum.", "content": "We present procedures for determining theophylline in 50 mul of serum. The drug is extracted into a small volume of solvent that contains an internal standard, 8-chlorotheophylline. The extract is Analyzed by isocratic reversedphase chromatography, with measurement of eluted theophylline at 273 nm. Day-to-day reproducibility within 5% is attainable for the concentration range 5--20 mg/liter. Other xanthines and related metabolites do not interfere. Sensitivity is 1 mg/liter. The correlation coefficient, when results by a spectrophotometric procedure were compared, was 0.989. Amobarbital, secobarbital, phenobarbital, and diphenylhydantoin do not interfere. Total analysis time for a single sample is 15 min.", "contents": "More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum. We present procedures for determining theophylline in 50 mul of serum. The drug is extracted into a small volume of solvent that contains an internal standard, 8-chlorotheophylline. The extract is Analyzed by isocratic reversedphase chromatography, with measurement of eluted theophylline at 273 nm. Day-to-day reproducibility within 5% is attainable for the concentration range 5--20 mg/liter. Other xanthines and related metabolites do not interfere. Sensitivity is 1 mg/liter. The correlation coefficient, when results by a spectrophotometric procedure were compared, was 0.989. Amobarbital, secobarbital, phenobarbital, and diphenylhydantoin do not interfere. Total analysis time for a single sample is 15 min.", "PMID": 975552}
{"id": "pubmed23n0032_10335", "title": "Gas-chromatographic identification of drugs in gastric aspirate samples: a rapid screening procedure for emergency toxicology.", "content": "Gastric aspirate has not been used frequently in the identification of ingested drugs. Because there is only qualitative significance in such a sample, a new method is described that facilitates identification. A 1-ml gastric aspirate sample is extracted with 100 mul of chloroform and analyzed directly by gas chromatography. Seventeen drugs commonly involved in overdose cases are included in this 25-min procedure. The gastric drug screen has been applied to more than 500 patients, and results are described.", "contents": "Gas-chromatographic identification of drugs in gastric aspirate samples: a rapid screening procedure for emergency toxicology. Gastric aspirate has not been used frequently in the identification of ingested drugs. Because there is only qualitative significance in such a sample, a new method is described that facilitates identification. A 1-ml gastric aspirate sample is extracted with 100 mul of chloroform and analyzed directly by gas chromatography. Seventeen drugs commonly involved in overdose cases are included in this 25-min procedure. The gastric drug screen has been applied to more than 500 patients, and results are described.", "PMID": 975553}
{"id": "pubmed23n0032_10336", "title": "Error introduced by specimen handling before determination of inorganic phosphate concentrations in plasma and serum.", "content": "Values for serum inorganic phosphate (Pi) concentrations in groups of healthy adults vary widely, the coefficient of variation ranging from 10 to 15%. We undertook to determine in 23 healthy adults whether part of this variation could be accounted for by (a) drawing blood in syringes vs. evacuated tubes (b) the time between blood sampling and separation of serum or plasma, and (c) the prevention of clotting. Values were unaffected by a, decreased significantly with time at room temperature between blood sampling and separation of cells in both serum and plasma, and were significantly lower in plasma than in serum. The group coefficient of variation for Pi averaged 13% and was uninfluenced by the blood-processing technique.", "contents": "Error introduced by specimen handling before determination of inorganic phosphate concentrations in plasma and serum. Values for serum inorganic phosphate (Pi) concentrations in groups of healthy adults vary widely, the coefficient of variation ranging from 10 to 15%. We undertook to determine in 23 healthy adults whether part of this variation could be accounted for by (a) drawing blood in syringes vs. evacuated tubes (b) the time between blood sampling and separation of serum or plasma, and (c) the prevention of clotting. Values were unaffected by a, decreased significantly with time at room temperature between blood sampling and separation of cells in both serum and plasma, and were significantly lower in plasma than in serum. The group coefficient of variation for Pi averaged 13% and was uninfluenced by the blood-processing technique.", "PMID": 975554}
{"id": "pubmed23n0032_10337", "title": "Improved thyroxine radioimmunoassay, for filter paper discs saturated with dried blood.", "content": "I describe an improved procedure for estimating thyroxine in dried blood on 0.32-cm filter paper discs, by radioimmunoassay. A weak anion-exchange resin is used to separate free and antibody-bound radiothyroxine and, by measuring the resin-bound 125I-labeled thyroxine rather than the supernatant radioactivity, a tedious and cumbersome step is eliminated. The unique properties of the resin permit considerable latitude in the experimental conditions, in contrast with charcoal, without compromising sensitivity or precision. There is excellent correlation between thyroxine values obtained from discs and those of the corresponding plasmas. Application of this procedure for large-scale screening of newborns has proved to be highly successful in identifying thyroid insufficiency in several babies before clinical signs were apparent.", "contents": "Improved thyroxine radioimmunoassay, for filter paper discs saturated with dried blood. I describe an improved procedure for estimating thyroxine in dried blood on 0.32-cm filter paper discs, by radioimmunoassay. A weak anion-exchange resin is used to separate free and antibody-bound radiothyroxine and, by measuring the resin-bound 125I-labeled thyroxine rather than the supernatant radioactivity, a tedious and cumbersome step is eliminated. The unique properties of the resin permit considerable latitude in the experimental conditions, in contrast with charcoal, without compromising sensitivity or precision. There is excellent correlation between thyroxine values obtained from discs and those of the corresponding plasmas. Application of this procedure for large-scale screening of newborns has proved to be highly successful in identifying thyroid insufficiency in several babies before clinical signs were apparent.", "PMID": 975555}
{"id": "pubmed23n0032_10338", "title": "Radioimmunuassay compared to thin-layer and gas--liquid chromatography for detecting methadone in human urine.", "content": "Radioimmunoassay was compared to thin-layer and gas--liquid chromatographic methods for detection of methadone in the urine of patients undergoing methadone maintenance therapy as treatment of heroin abuse. With urine samples known to contain methadone, 84% were positive by thin-layer chromatography as compared to 99% positives by the other two methods. This difference is attributed to the difference in sensitivity of the three methods. All three methods gave consistently positive results with urine samples from patients receiving 25 mg of methadone per day or more. With smaller daily doses the percentage of positive results obtained with thin-layer chromatography decreased. Analysis of urine samples not containing methadone showed no incidence of cross reaction of other drugs with the methadone radioimmunoassay. The methadone radioimmunoassay appears to be both sensitive and reliable; however, certain other factors limit its use as a primary screening method.", "contents": "Radioimmunuassay compared to thin-layer and gas--liquid chromatography for detecting methadone in human urine. Radioimmunoassay was compared to thin-layer and gas--liquid chromatographic methods for detection of methadone in the urine of patients undergoing methadone maintenance therapy as treatment of heroin abuse. With urine samples known to contain methadone, 84% were positive by thin-layer chromatography as compared to 99% positives by the other two methods. This difference is attributed to the difference in sensitivity of the three methods. All three methods gave consistently positive results with urine samples from patients receiving 25 mg of methadone per day or more. With smaller daily doses the percentage of positive results obtained with thin-layer chromatography decreased. Analysis of urine samples not containing methadone showed no incidence of cross reaction of other drugs with the methadone radioimmunoassay. The methadone radioimmunoassay appears to be both sensitive and reliable; however, certain other factors limit its use as a primary screening method.", "PMID": 975556}
{"id": "pubmed23n0032_10339", "title": "Relationship between erythrocyte 2,3-diphosphoglycerate and age in a normal population.", "content": "The concentration of 2,3-diphosphoglycerate in erythrocytes was determined in 369 male and 376 female normal subjects, 1 to 86 years of age, derived from the Greek population. It was inversely correlated (P less than 0.01) with age. Persons over age 60 showed a decrease (P less than 0.05) in 2,3-diphosphoglycerate concentrations as compared those 1-20 years of age.", "contents": "Relationship between erythrocyte 2,3-diphosphoglycerate and age in a normal population. The concentration of 2,3-diphosphoglycerate in erythrocytes was determined in 369 male and 376 female normal subjects, 1 to 86 years of age, derived from the Greek population. It was inversely correlated (P less than 0.01) with age. Persons over age 60 showed a decrease (P less than 0.05) in 2,3-diphosphoglycerate concentrations as compared those 1-20 years of age.", "PMID": 975557}
{"id": "pubmed23n0032_10340", "title": "[Colorimetric determination of calcium in serum using methylthymol blue].", "content": "A simple, specific and rapid colorimetric method for the determination of calcium concentration and serum using methylthymol blue is described. The reagent given can be used both in a manual method and in an automatic method using an auto-analyser without dialysis. The results obtained by the automatic method are compared to those found by the manual method and those obtained by atomic absorption.", "contents": "[Colorimetric determination of calcium in serum using methylthymol blue]. A simple, specific and rapid colorimetric method for the determination of calcium concentration and serum using methylthymol blue is described. The reagent given can be used both in a manual method and in an automatic method using an auto-analyser without dialysis. The results obtained by the automatic method are compared to those found by the manual method and those obtained by atomic absorption.", "PMID": 975564}
{"id": "pubmed23n0032_10341", "title": "Accumulation of lactosyl ceramide in leukocytes of patients with adult Gaucher's disease.", "content": "Glycosphingolipids were isolated from the leukocytes of 10 patients with Type I, chronic nonneuronopathic (adult) Gaucher's disease and 12 normal subjects, by silicic acid column chromatography and thin-layer chromatography. Quantitation of the individual glycosyl ceramides was achieved by the determination of hexose and sphingosine content, using colorimetric and fluorometric procedures. Lactosyl ceramide, which is the main glycolipid in leukocytes of normal subjects, was significantly increased in the leukocytes of patients with Gaucher's disease. On the other hand, the amount of glycosyl ceramide, which is the main glycolipid accumulating in the reticuloendothelial cells of patients with Gaucher's disease, was similar in Gaucher and in control leukocytes.", "contents": "Accumulation of lactosyl ceramide in leukocytes of patients with adult Gaucher's disease. Glycosphingolipids were isolated from the leukocytes of 10 patients with Type I, chronic nonneuronopathic (adult) Gaucher's disease and 12 normal subjects, by silicic acid column chromatography and thin-layer chromatography. Quantitation of the individual glycosyl ceramides was achieved by the determination of hexose and sphingosine content, using colorimetric and fluorometric procedures. Lactosyl ceramide, which is the main glycolipid in leukocytes of normal subjects, was significantly increased in the leukocytes of patients with Gaucher's disease. On the other hand, the amount of glycosyl ceramide, which is the main glycolipid accumulating in the reticuloendothelial cells of patients with Gaucher's disease, was similar in Gaucher and in control leukocytes.", "PMID": 975565}
{"id": "pubmed23n0032_10342", "title": "gamma-Carboxyglutamic acid in human urine.", "content": "gamma-Carboxyglutamic acid, the amino acid responsible for the vitamin K dependent, Ca2+-binding structures of some of the blood coagulation proteins, has been identified in human urine. The amino acid wasisolated and its identity was proved by comparing it with synthetic gamma-carboxyglutamic acid by electrophoretic and chromatographic methods and by mass spectrometry. The isolated compound was also converted to glutamic acid by heat decarboxylation, a reaction consistent with its anticipated structure. A method for the quantitation of free gamma-carboxyglutamic acid in human urine was developed. The method consisted of an anion-exchange concentration step followed by automatic amino acid analysis using a pH 2.0 lithium citrate buffer. In three non-fasting adult males the urinary excretion of gamma-carboxyglutamic acid ranged between 27 and 42 mumol/24 h and in six non-fasting adult females it ranged between 19 and 32 mumol/24 h. One fasting adult male excreted 36 mumol/24 h.", "contents": "gamma-Carboxyglutamic acid in human urine. gamma-Carboxyglutamic acid, the amino acid responsible for the vitamin K dependent, Ca2+-binding structures of some of the blood coagulation proteins, has been identified in human urine. The amino acid wasisolated and its identity was proved by comparing it with synthetic gamma-carboxyglutamic acid by electrophoretic and chromatographic methods and by mass spectrometry. The isolated compound was also converted to glutamic acid by heat decarboxylation, a reaction consistent with its anticipated structure. A method for the quantitation of free gamma-carboxyglutamic acid in human urine was developed. The method consisted of an anion-exchange concentration step followed by automatic amino acid analysis using a pH 2.0 lithium citrate buffer. In three non-fasting adult males the urinary excretion of gamma-carboxyglutamic acid ranged between 27 and 42 mumol/24 h and in six non-fasting adult females it ranged between 19 and 32 mumol/24 h. One fasting adult male excreted 36 mumol/24 h.", "PMID": 975566}
{"id": "pubmed23n0032_10343", "title": "A system of interpretation of infrared spectra of calculi for routine use in the clinical laboratory.", "content": "We have devised systematic schemes for objectives qualitative analysis of the IR spectra of calculi. These schemes are presented as flow charts based on questions each requiring a yes or no answer before going on to the next step. The specific question asked in each step concerns the similarity of a limited region of the spectrum to reference spectra. For renal calculi, 5 regions are evaluated and a maximum of 10 decisions is necessary to complete the analysis. Biliary calculi are treated similarily, with 5 spectral regions evaluated and a maximum of 8 yes-no decisions required. The method requires less technician time and is much less susceptible to differences in subjective judgment than the wet chemical procedure. Three percent of stones contain unusual components and are quickly recognized as such and identified using a library of reference spectra.", "contents": "A system of interpretation of infrared spectra of calculi for routine use in the clinical laboratory. We have devised systematic schemes for objectives qualitative analysis of the IR spectra of calculi. These schemes are presented as flow charts based on questions each requiring a yes or no answer before going on to the next step. The specific question asked in each step concerns the similarity of a limited region of the spectrum to reference spectra. For renal calculi, 5 regions are evaluated and a maximum of 10 decisions is necessary to complete the analysis. Biliary calculi are treated similarily, with 5 spectral regions evaluated and a maximum of 8 yes-no decisions required. The method requires less technician time and is much less susceptible to differences in subjective judgment than the wet chemical procedure. Three percent of stones contain unusual components and are quickly recognized as such and identified using a library of reference spectra.", "PMID": 975570}
{"id": "pubmed23n0032_10344", "title": "Optimization of T assay: a model study.", "content": "Factors involved in the optimization of competitive binding radioassays have been analyzed using a thyroxine (T4) radioassay as a model system. The effects of kinetic errors are minimized by the use of the lowest possible incubation temperature for the separation step and by the use of labeled T4 of high purity (which is not consistently available from commerical sources). The use of labeled T4 containing significant quantities of labeled triiodothyronine (T3) can lead to a marked increase in bias and a decrease in precision with relatively small errors in the separation step due to the relatively short half-life of the T3-binder complex. The determination of the dissociation rate constant (not to be confused with the affinity constant) allows one to make useful estimates of the tolerances that can be allowed in the separation step in order to achieve a  desired level of accuracy and precison.", "contents": "Optimization of T assay: a model study. Factors involved in the optimization of competitive binding radioassays have been analyzed using a thyroxine (T4) radioassay as a model system. The effects of kinetic errors are minimized by the use of the lowest possible incubation temperature for the separation step and by the use of labeled T4 of high purity (which is not consistently available from commerical sources). The use of labeled T4 containing significant quantities of labeled triiodothyronine (T3) can lead to a marked increase in bias and a decrease in precision with relatively small errors in the separation step due to the relatively short half-life of the T3-binder complex. The determination of the dissociation rate constant (not to be confused with the affinity constant) allows one to make useful estimates of the tolerances that can be allowed in the separation step in order to achieve a  desired level of accuracy and precison.", "PMID": 975571}
{"id": "pubmed23n0032_10345", "title": "An isolation system for aldosterone and tetrahydroaldosterone prior to estimation by gas liquid chromatography or radioimmunoassay.", "content": "We report a method of isolation of aldosterone and tetrahydroaldosterone from urine and undiluted plasma which is based on extraction with Amberlite XAD-2 resin and purification by low pressure liquid chromatography using microbore LH-20 columns. Thin-layer chromatography on polyamide plates is introduced as the final step of a procedure designed to reduce destruction of steroids to a minimum, and to provide, in addition, a facility for multisample processing. Using a synthetic substrate, it has been shown that enzyme hydrolysis of steroid conjugates is possible whilst still adsorbed to the resin after extraction although the yield is lower than when hydrolysis is performed in solution.", "contents": "An isolation system for aldosterone and tetrahydroaldosterone prior to estimation by gas liquid chromatography or radioimmunoassay. We report a method of isolation of aldosterone and tetrahydroaldosterone from urine and undiluted plasma which is based on extraction with Amberlite XAD-2 resin and purification by low pressure liquid chromatography using microbore LH-20 columns. Thin-layer chromatography on polyamide plates is introduced as the final step of a procedure designed to reduce destruction of steroids to a minimum, and to provide, in addition, a facility for multisample processing. Using a synthetic substrate, it has been shown that enzyme hydrolysis of steroid conjugates is possible whilst still adsorbed to the resin after extraction although the yield is lower than when hydrolysis is performed in solution.", "PMID": 975573}
{"id": "pubmed23n0032_10346", "title": "Solid-phase radioimmunoassay of plasma triiodothyronine.", "content": "A direct radioimmunoassay method for circulating triiodothyronine was set up, using an insolubilized (cellulose-coupled) antiserum and 8-anilino-1-naphthalene-sulfonic acid (ANS) as a protein inhibitor. Particular attention was paid to the evaluation of changes in the physico-chemical properties of the antibody sites under the experimental conditions adopted: no substantial decrease of immunoreactivity was found to be associated with the insolubilization process, while complex effects following the use of protein inhibitors were observed. The standardized procedure was defined in terms of analytical reliability both by a comparison with a reference (charcoal-dextran) method and by evaluation of sensitivity, precision and accuracy.", "contents": "Solid-phase radioimmunoassay of plasma triiodothyronine. A direct radioimmunoassay method for circulating triiodothyronine was set up, using an insolubilized (cellulose-coupled) antiserum and 8-anilino-1-naphthalene-sulfonic acid (ANS) as a protein inhibitor. Particular attention was paid to the evaluation of changes in the physico-chemical properties of the antibody sites under the experimental conditions adopted: no substantial decrease of immunoreactivity was found to be associated with the insolubilization process, while complex effects following the use of protein inhibitors were observed. The standardized procedure was defined in terms of analytical reliability both by a comparison with a reference (charcoal-dextran) method and by evaluation of sensitivity, precision and accuracy.", "PMID": 975574}
{"id": "pubmed23n0032_10347", "title": "Rapid and effective separation of thyroid hormones and iodide by thin-layer chromatography.", "content": "The use of 1,3-dichlorotetrafluoroacetone allows the conversion of the iodoamino acids into a chemical form convenient for following chromatographic separation on silica as an adsorbent and benzene or chloroform as a solvent. The time required for derivatization and TLC separation is less than one hour. The outstanding resolution of the substance of interest, as well as the sensitivity of the radiochemical analysis, makes the method convenient for the rapid determination of minute quantities of thyroid hormones.", "contents": "Rapid and effective separation of thyroid hormones and iodide by thin-layer chromatography. The use of 1,3-dichlorotetrafluoroacetone allows the conversion of the iodoamino acids into a chemical form convenient for following chromatographic separation on silica as an adsorbent and benzene or chloroform as a solvent. The time required for derivatization and TLC separation is less than one hour. The outstanding resolution of the substance of interest, as well as the sensitivity of the radiochemical analysis, makes the method convenient for the rapid determination of minute quantities of thyroid hormones.", "PMID": 975575}
{"id": "pubmed23n0032_10348", "title": "Comparison of a simplified radioimmunoassay with a gas-liquid chromatographic method for urinary aldosterone.", "content": "A simplified radioimmunoassay for the measurement of aldosterone in urine, requiring only an extraction without any further purification is described. The results obtained on 47 urine specimens were compared with those obtained by a gas-liquid chromatographic method. An excellent correlation was found (r = 0.932) and values obtained by both methods were similar so that this RIA has been satisfactorily adopted in our laboratory for one year. Some discrepancies, however, led us to consider the problem of preservation and storage of biological fluids.", "contents": "Comparison of a simplified radioimmunoassay with a gas-liquid chromatographic method for urinary aldosterone. A simplified radioimmunoassay for the measurement of aldosterone in urine, requiring only an extraction without any further purification is described. The results obtained on 47 urine specimens were compared with those obtained by a gas-liquid chromatographic method. An excellent correlation was found (r = 0.932) and values obtained by both methods were similar so that this RIA has been satisfactorily adopted in our laboratory for one year. Some discrepancies, however, led us to consider the problem of preservation and storage of biological fluids.", "PMID": 975576}
{"id": "pubmed23n0032_10349", "title": "The effect of a short burst of exercise on activity values of enzymes in sera of healthy young men.", "content": "We report the effect of exercise on the activity values of five enzymes in sera as studied in four healthy male volunteers. The underlying purpose of this present study was to produce an increase in the activity values in the sera of selected enzymes found in muscle. Then by observing the decay rate of these enzymes, we computed the inter-individual differences in clearance rates serum half-life) of these enzymes. Blood specimens were collected just prior to exercise, 1 h after excerise, and on eight additional times up to 93 h after exercise. All specimens were assayed on one occasion for activity values of creatine kinase, asparate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase. We found increases in the three muscle enzymes with average increases being: creatine kinase, +116%; asparate aminotransferase, +41%; and lactate dehydrogenase, +32%; all of which remained above baseline values for 53 h or longer. In the case of creatine kinase, a monoexponential decay curve depicted the data (from the 19-h specimen to the 67-h specimen). The calculated \"apparent serum half-life\" for creatine kinase varied from 38 h to 118 h in the subjects tested.", "contents": "The effect of a short burst of exercise on activity values of enzymes in sera of healthy young men. We report the effect of exercise on the activity values of five enzymes in sera as studied in four healthy male volunteers. The underlying purpose of this present study was to produce an increase in the activity values in the sera of selected enzymes found in muscle. Then by observing the decay rate of these enzymes, we computed the inter-individual differences in clearance rates serum half-life) of these enzymes. Blood specimens were collected just prior to exercise, 1 h after excerise, and on eight additional times up to 93 h after exercise. All specimens were assayed on one occasion for activity values of creatine kinase, asparate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase. We found increases in the three muscle enzymes with average increases being: creatine kinase, +116%; asparate aminotransferase, +41%; and lactate dehydrogenase, +32%; all of which remained above baseline values for 53 h or longer. In the case of creatine kinase, a monoexponential decay curve depicted the data (from the 19-h specimen to the 67-h specimen). The calculated \"apparent serum half-life\" for creatine kinase varied from 38 h to 118 h in the subjects tested.", "PMID": 975577}
{"id": "pubmed23n0032_10350", "title": "An assessment of the clinical usefulness of plasma ribonuclease assays.", "content": "The usefulness of plasma ribonuclease assays was studied in (i) patients with possible protein deficiency, (ii) patients with myelomatosis, (iii) patients with carcinoma of the breast. In each group, the major factor associated with elevation of plasma ribonuclease was impairment of renal function. The assay was therefore of little value in the assessment of patients with myelomatosis or carcinoma of the breast. However, in the patients with possible protein deficiency and normal renal function, an elevation of plasma ribonuclease is, in general, associated with a decrease in serum albumin, transferrin and cholinesterase. Plasma ribonuclease may therefore be a useful parameter in the assessment of protein nutritional status.", "contents": "An assessment of the clinical usefulness of plasma ribonuclease assays. The usefulness of plasma ribonuclease assays was studied in (i) patients with possible protein deficiency, (ii) patients with myelomatosis, (iii) patients with carcinoma of the breast. In each group, the major factor associated with elevation of plasma ribonuclease was impairment of renal function. The assay was therefore of little value in the assessment of patients with myelomatosis or carcinoma of the breast. However, in the patients with possible protein deficiency and normal renal function, an elevation of plasma ribonuclease is, in general, associated with a decrease in serum albumin, transferrin and cholinesterase. Plasma ribonuclease may therefore be a useful parameter in the assessment of protein nutritional status.", "PMID": 975578}
{"id": "pubmed23n0032_10351", "title": "Pregnancy associated alpha2-glycoprotein in post-menopausal women receiving hormone replacement therapy.", "content": "Serum pregnancy associated alpha2-glycoprotein (P.A.G.) concentrations were measured in a group of post-menopausal women receiving hormone replacement therapy. The P.A.G. concentrations increased from a mean basal level of 48.1 mug/ml (range 20.6--104.4 mug/ml) to a maximum after six months therapy of 359 mug/ml (range 82--1290 mug/ml). This represents a mean increase of 647%. Two months after cessation of therapy the P.A.G. concentrations decreased but the mean level (60.3 mug/ml) was still significantly higher than the pre-treatment level. There was a significant correlation between the pre-treatment P.A.G. concentrations and the levels after six months of therapy. These findings may be of relevance to the use of P.A.G. determinations in the monitoring of malignant disease.", "contents": "Pregnancy associated alpha2-glycoprotein in post-menopausal women receiving hormone replacement therapy. Serum pregnancy associated alpha2-glycoprotein (P.A.G.) concentrations were measured in a group of post-menopausal women receiving hormone replacement therapy. The P.A.G. concentrations increased from a mean basal level of 48.1 mug/ml (range 20.6--104.4 mug/ml) to a maximum after six months therapy of 359 mug/ml (range 82--1290 mug/ml). This represents a mean increase of 647%. Two months after cessation of therapy the P.A.G. concentrations decreased but the mean level (60.3 mug/ml) was still significantly higher than the pre-treatment level. There was a significant correlation between the pre-treatment P.A.G. concentrations and the levels after six months of therapy. These findings may be of relevance to the use of P.A.G. determinations in the monitoring of malignant disease.", "PMID": 975579}
{"id": "pubmed23n0032_10352", "title": "Radioimmunoassay for aldosterone and deoxycorticosterone in human plasma. Comparison of various antisera and determination of normal values.", "content": "This paper describes a study of the physico-chemical and radioimmunological properties of three antialdosterone antisera which permitted practical conclusions to be drawn. By its high degree of specificity, anti-aldo-3-oxime-BSA constitutes the most useful antiserum for the clinical assay of aldosterone. The principal advantage of this antiserum is that it allows both urinary and blood aldosterone radioimmunoassay without the necessity of including a chromatographic step. No problems arise with the blanks. This work also includes the study of two anti-deoxycorticosterone antisera. The aldosterone and deoxycorticosterone values, obtained from normal subjects under various physiological conditions, are in agreement with the values given in the literature.", "contents": "Radioimmunoassay for aldosterone and deoxycorticosterone in human plasma. Comparison of various antisera and determination of normal values. This paper describes a study of the physico-chemical and radioimmunological properties of three antialdosterone antisera which permitted practical conclusions to be drawn. By its high degree of specificity, anti-aldo-3-oxime-BSA constitutes the most useful antiserum for the clinical assay of aldosterone. The principal advantage of this antiserum is that it allows both urinary and blood aldosterone radioimmunoassay without the necessity of including a chromatographic step. No problems arise with the blanks. This work also includes the study of two anti-deoxycorticosterone antisera. The aldosterone and deoxycorticosterone values, obtained from normal subjects under various physiological conditions, are in agreement with the values given in the literature.", "PMID": 975582}
{"id": "pubmed23n0032_10353", "title": "Effect of fatty acids on thyroid function tests in vitro and in vivo.", "content": "Addition of long-chain fatty acids to serum increased thyroxine (T4), measured by a competitive protein binding assay, and triiodothyronine (T3) uptake by Sephadex or resin (T3U tests). This is compatible with the assumption that fatty acids compete with thyroxine for binding sites on T4-binding proteins. When equimolar concentrations of various saturated and unsaturated fatty acids were added to serum it was observed that the effectiveness in raising tests based on protein binding of thyroid hormones incrreased serum T3 determined by radioimmunoassay (RIA). T4(RIA) was not significantly influenced by either saturated or unsaturated fatty acids. Serum T4(CPB) rose during storage at 22degreesC and 37degreesC but was stable at 4degreesC and --20degreesC for periods up to two weeks. The proportional increase in T4(CPB) and free fatty acids (FFA) indicated that this phenomenon was due, at least partly, to the interference from FFA formed during storage of the serum. There was also a small, significant increase in T3U, T3(RIA) and CT4I (a free thyroxine estimate) after storage of serum at room temperature or higher for one to two weeks. Serum T4(RIA) did not alter during two weeks of storage. In five subjects with raised serum FFA after eating a fat meal followed by a heparin injection an increase in T4(CPB), T3U, T3(RIA) and CT4I that was proportional to the increase in FFA was observed. This effect on the thyroid tests was small until the increase in FFA concentration exceeded 2 mmol/l. T4(RIA) did not respond to the increase in FFA. In ten patients with raised levels of FFA due to uncontrolled diabetes T4(CPB), T4(RIA) and T3(RIA) decreased while T3U increased. These unexpected alterations were probably related to the severe, chronic illness in these patients. Increased FFA in vivo seem to be of little importance for the interpretation of thyroid tests in clinical practice.", "contents": "Effect of fatty acids on thyroid function tests in vitro and in vivo. Addition of long-chain fatty acids to serum increased thyroxine (T4), measured by a competitive protein binding assay, and triiodothyronine (T3) uptake by Sephadex or resin (T3U tests). This is compatible with the assumption that fatty acids compete with thyroxine for binding sites on T4-binding proteins. When equimolar concentrations of various saturated and unsaturated fatty acids were added to serum it was observed that the effectiveness in raising tests based on protein binding of thyroid hormones incrreased serum T3 determined by radioimmunoassay (RIA). T4(RIA) was not significantly influenced by either saturated or unsaturated fatty acids. Serum T4(CPB) rose during storage at 22degreesC and 37degreesC but was stable at 4degreesC and --20degreesC for periods up to two weeks. The proportional increase in T4(CPB) and free fatty acids (FFA) indicated that this phenomenon was due, at least partly, to the interference from FFA formed during storage of the serum. There was also a small, significant increase in T3U, T3(RIA) and CT4I (a free thyroxine estimate) after storage of serum at room temperature or higher for one to two weeks. Serum T4(RIA) did not alter during two weeks of storage. In five subjects with raised serum FFA after eating a fat meal followed by a heparin injection an increase in T4(CPB), T3U, T3(RIA) and CT4I that was proportional to the increase in FFA was observed. This effect on the thyroid tests was small until the increase in FFA concentration exceeded 2 mmol/l. T4(RIA) did not respond to the increase in FFA. In ten patients with raised levels of FFA due to uncontrolled diabetes T4(CPB), T4(RIA) and T3(RIA) decreased while T3U increased. These unexpected alterations were probably related to the severe, chronic illness in these patients. Increased FFA in vivo seem to be of little importance for the interpretation of thyroid tests in clinical practice.", "PMID": 975583}
{"id": "pubmed23n0032_10354", "title": "A simple quantitative method to determine short chain fatty acid levels in biological fluids.", "content": "A simple method has been developed for quantitative acetic, propionic, isobutyric, butyric, isovaleric, valeric, isocaproic, and caproic acids in intestinal fluids, feces and blood. The method utilizes extraction with ether and gas chromatography. It is accurate over a wide range of SCFA concentrations and appears to be applicable to any biological fluid.", "contents": "A simple quantitative method to determine short chain fatty acid levels in biological fluids. A simple method has been developed for quantitative acetic, propionic, isobutyric, butyric, isovaleric, valeric, isocaproic, and caproic acids in intestinal fluids, feces and blood. The method utilizes extraction with ether and gas chromatography. It is accurate over a wide range of SCFA concentrations and appears to be applicable to any biological fluid.", "PMID": 975584}
{"id": "pubmed23n0032_10355", "title": "The quantitation of plasma phytanic acid by mass fragmentography.", "content": "Plasma phytanic acid has been quantitated by mass fragmentography after its successive reduction and derivatization to the corresponding t-butyldimethylsilyl ether. The latter was estimated by selective ion monitoring of its characteristic (M-57) ion (M/z 355). The technique has the advantage of being more rapid, specific and sensitive than existing methods, permitting the determination of phytanic acid at levels greater than 5 mug/ml plasma.", "contents": "The quantitation of plasma phytanic acid by mass fragmentography. Plasma phytanic acid has been quantitated by mass fragmentography after its successive reduction and derivatization to the corresponding t-butyldimethylsilyl ether. The latter was estimated by selective ion monitoring of its characteristic (M-57) ion (M/z 355). The technique has the advantage of being more rapid, specific and sensitive than existing methods, permitting the determination of phytanic acid at levels greater than 5 mug/ml plasma.", "PMID": 975585}
{"id": "pubmed23n0032_10356", "title": "Pharmacological influences on T4 to T3 conversion in rat liver.", "content": "Five groups of 8 rats were treated with normal diet, diphenylhydantoin, dexamethasone, thyroxine (T4) and phenobarbital respectively for 14 days. After this period plasma triiodothyronine (T3), T4 and reverse T3 (rT3) concentrations as well as the T4 to T3 converting activity in the liver homogenates were determined. Plasma T3 was low after dexamethasone, T4 and phenobarbital. Plasma T4 was low after dexamethasone and elevated after T4. rT3 plasma concentrations were at the lower limit of detection in all groups. The converting activity in the liver homogenate was significantly increased after diphenylhydantoin and very much depressed after dexamethasone. These results support the concept that different drugs may influence the peripheral thyroid hormone metabolism, especially the T4 to T3 conversion. However other pathways might be affected also because plasma T3 levels did not always reflect the converting activity in the liver.", "contents": "Pharmacological influences on T4 to T3 conversion in rat liver. Five groups of 8 rats were treated with normal diet, diphenylhydantoin, dexamethasone, thyroxine (T4) and phenobarbital respectively for 14 days. After this period plasma triiodothyronine (T3), T4 and reverse T3 (rT3) concentrations as well as the T4 to T3 converting activity in the liver homogenates were determined. Plasma T3 was low after dexamethasone, T4 and phenobarbital. Plasma T4 was low after dexamethasone and elevated after T4. rT3 plasma concentrations were at the lower limit of detection in all groups. The converting activity in the liver homogenate was significantly increased after diphenylhydantoin and very much depressed after dexamethasone. These results support the concept that different drugs may influence the peripheral thyroid hormone metabolism, especially the T4 to T3 conversion. However other pathways might be affected also because plasma T3 levels did not always reflect the converting activity in the liver.", "PMID": 975586}
{"id": "pubmed23n0032_10357", "title": "The use of mass fragmentography in the evaluation of routine methods for glucose determination.", "content": "A highly specific and accurate mass fragmentographic reference method for determination of serum glucose is described. A fixed amount of hepta-deuterated glucose is added to a fixed amount of serum. The mixture is lyophilized, converted into the penta-trimethylsilyl-methyloxime derivative and subjected to analysis with a combined gas chromatograph - mass spectrometer equipped with a MID-unit (multiple ion detector). The amount of unlabeled glucose was determined from the ratio between recordings at m/e 319 and 323. The two ions used correspond to the base peak in the mass spectrum of derivative of unlabeled and hepta-deuterium labeled glucose, respectively. The relative standard deviation of the method was 1.9%. The method was compared with different enzymatic methods based on the hexokinase reaction and the glucose oxidase reaction. Of the different methods tested, a glucose oxidase method with determination of maximal rate of consumption of oxygen gave results most close to the results of the reference method.", "contents": "The use of mass fragmentography in the evaluation of routine methods for glucose determination. A highly specific and accurate mass fragmentographic reference method for determination of serum glucose is described. A fixed amount of hepta-deuterated glucose is added to a fixed amount of serum. The mixture is lyophilized, converted into the penta-trimethylsilyl-methyloxime derivative and subjected to analysis with a combined gas chromatograph - mass spectrometer equipped with a MID-unit (multiple ion detector). The amount of unlabeled glucose was determined from the ratio between recordings at m/e 319 and 323. The two ions used correspond to the base peak in the mass spectrum of derivative of unlabeled and hepta-deuterium labeled glucose, respectively. The relative standard deviation of the method was 1.9%. The method was compared with different enzymatic methods based on the hexokinase reaction and the glucose oxidase reaction. Of the different methods tested, a glucose oxidase method with determination of maximal rate of consumption of oxygen gave results most close to the results of the reference method.", "PMID": 975587}
{"id": "pubmed23n0032_10358", "title": "A diagnostic triad for portal cirrhosis.", "content": "Abnormal serum aminotransferase activities with dominance of aspartate aminotransferase over alanine aminotransferase activity, and elevated serum adenosine deaminase activity and immunoglobulin. A concentration, were commonly encountered among patients with portal cirrhosis. The full triad was present in 31 of 49 cases (63%). As isolated abnormalities, these features were not uncommon in patients with other diseases of the liver and biliary tree, but the full triad was found only in 11 of 163 such cases (6.8%). The presence of this triad in a patient with unexplained hepatomegaly is indicative of portal cirrhosis.", "contents": "A diagnostic triad for portal cirrhosis. Abnormal serum aminotransferase activities with dominance of aspartate aminotransferase over alanine aminotransferase activity, and elevated serum adenosine deaminase activity and immunoglobulin. A concentration, were commonly encountered among patients with portal cirrhosis. The full triad was present in 31 of 49 cases (63%). As isolated abnormalities, these features were not uncommon in patients with other diseases of the liver and biliary tree, but the full triad was found only in 11 of 163 such cases (6.8%). The presence of this triad in a patient with unexplained hepatomegaly is indicative of portal cirrhosis.", "PMID": 975589}
{"id": "pubmed23n0032_10359", "title": "Assay of plasma 25-hydroxy vitamin D.", "content": "A radio-competitive protein-binding assay for 25-hydroxy vitamin D3 (25-OHD3) is described using diluted plasma from patients with vitamin D deficient osteomalacia as the binding protein. The 25-OHD3 is extracted from plasma with ethanol and assayed directly without further purification. An incubation of two hours is used to give suitable binding. The lower detection limit of the assay is approximately 23 pg, the intra-assay coefficient of variation is 5.4% and the inter-assay variation 15%. Tth a mean of 26.9 mug/l. Levels of plasma 25-OHD3 are undetectable in patients with vitamin D deficient osteomalacia and greater than 110 mug/l in patients taking large doses of vitamin D2.", "contents": "Assay of plasma 25-hydroxy vitamin D. A radio-competitive protein-binding assay for 25-hydroxy vitamin D3 (25-OHD3) is described using diluted plasma from patients with vitamin D deficient osteomalacia as the binding protein. The 25-OHD3 is extracted from plasma with ethanol and assayed directly without further purification. An incubation of two hours is used to give suitable binding. The lower detection limit of the assay is approximately 23 pg, the intra-assay coefficient of variation is 5.4% and the inter-assay variation 15%. Tth a mean of 26.9 mug/l. Levels of plasma 25-OHD3 are undetectable in patients with vitamin D deficient osteomalacia and greater than 110 mug/l in patients taking large doses of vitamin D2.", "PMID": 975590}
{"id": "pubmed23n0032_10360", "title": "The level and composition of seromucoid and nondialysable serum sugar-peptide fraction compared with the activity of galactosyl transferase (EC 2.4.1.38) of liver Golgi membranes in insulin-treated rats.", "content": "Long-term treatment of rats with insulin does not influence the yield of the Golgi membrane-rich fraction obtained by Fleischers' method or the activity of galactosyl transferase (EC 2.1.1.38) in present in this fraction. Exogenous insulin increases the total levels of the seromucoid and serum middle-weight molecular glycopeptide fraction, but does not alter the proportions of neutral sugars bound with the peptide residues of both fractions.", "contents": "The level and composition of seromucoid and nondialysable serum sugar-peptide fraction compared with the activity of galactosyl transferase (EC 2.4.1.38) of liver Golgi membranes in insulin-treated rats. Long-term treatment of rats with insulin does not influence the yield of the Golgi membrane-rich fraction obtained by Fleischers' method or the activity of galactosyl transferase (EC 2.1.1.38) in present in this fraction. Exogenous insulin increases the total levels of the seromucoid and serum middle-weight molecular glycopeptide fraction, but does not alter the proportions of neutral sugars bound with the peptide residues of both fractions.", "PMID": 975591}
{"id": "pubmed23n0032_10361", "title": "Clinical studies in benign (Becker type) X-linked muscular dystrophy.", "content": "Ten extensive families with benign (Becker type) X-linked muscular dystrophy have been studied, in which there was a total of 67 affected males. Reliable information was available on 41 of these males, 29 of whom were alive at the time of the study. The disorder was characterised by a predominantly proximal myopathy, associated with pseudohypertrophy of the calf muscles, particularly in the early stages of the disease. The early development of contractures was not a feature of the disease in these families and cardiac involvement, when present, was a late manifestation. The clinical findings in these patients are discussed in detail and compared with those in a large unselected group of patients with Duchenne muscular dystrophy. The results indicate that the best criterion for distinguishing between these two disorders is the age of becoming chair-bound. Electrocardiographic studies revealed no consistent abnormality and no evidence of an abnormal algebraic sum of the R and S waves in lead V1, as is found in patients with Duchenne muscular dystrophy. Serum creatine kinase levels are significantly elevated, particularly in the early stages of the disease, and in this way preclinical cases may be identified.", "contents": "Clinical studies in benign (Becker type) X-linked muscular dystrophy. Ten extensive families with benign (Becker type) X-linked muscular dystrophy have been studied, in which there was a total of 67 affected males. Reliable information was available on 41 of these males, 29 of whom were alive at the time of the study. The disorder was characterised by a predominantly proximal myopathy, associated with pseudohypertrophy of the calf muscles, particularly in the early stages of the disease. The early development of contractures was not a feature of the disease in these families and cardiac involvement, when present, was a late manifestation. The clinical findings in these patients are discussed in detail and compared with those in a large unselected group of patients with Duchenne muscular dystrophy. The results indicate that the best criterion for distinguishing between these two disorders is the age of becoming chair-bound. Electrocardiographic studies revealed no consistent abnormality and no evidence of an abnormal algebraic sum of the R and S waves in lead V1, as is found in patients with Duchenne muscular dystrophy. Serum creatine kinase levels are significantly elevated, particularly in the early stages of the disease, and in this way preclinical cases may be identified.", "PMID": 975594}
{"id": "pubmed23n0032_10362", "title": "X-short arm deletion gonadal dysgenesis in two siblings due to unique translocation (Xp-;16p+).", "content": "A family demonstrating short arm deletion of the X chromosome as a consequence of X-16 balanced translocation in the mother is reported. The two Xp- sisters exhibit clinical signs of gonadal dysgenesis, while the balanced carriers are phenotypically normal. To our knowledge this represents the only example of both the balanced carrier state for an X translocation and its genetic consequence occurring in the offspring, as well as the involvement of X-16 interchange. Literature data of 37 additional cases of verified X translocations are discussed.", "contents": "X-short arm deletion gonadal dysgenesis in two siblings due to unique translocation (Xp-;16p+). A family demonstrating short arm deletion of the X chromosome as a consequence of X-16 balanced translocation in the mother is reported. The two Xp- sisters exhibit clinical signs of gonadal dysgenesis, while the balanced carriers are phenotypically normal. To our knowledge this represents the only example of both the balanced carrier state for an X translocation and its genetic consequence occurring in the offspring, as well as the involvement of X-16 interchange. Literature data of 37 additional cases of verified X translocations are discussed.", "PMID": 975595}
{"id": "pubmed23n0032_10363", "title": "Infantile XX male: a case report.", "content": "A case of infantile XX male syndrome with bilateral scrotal testes and penoscrotal hypospadias is presented. No evidence of XX/XY mosaicism of Y chromatin was obtained in preparations from cultures of the peripheral blood, skin fibroblast, or other tissues. Although true hermaphroditism was suspected, exploration of the bilateral gonadal structures failed to detect the presence of ovarian structures, either grossly or microscopically. Furthermore, exploratory laparotomy revealed no Mullerian structures. The difficulty of early diagnosis of XX males in infancy is emphasized.", "contents": "Infantile XX male: a case report. A case of infantile XX male syndrome with bilateral scrotal testes and penoscrotal hypospadias is presented. No evidence of XX/XY mosaicism of Y chromatin was obtained in preparations from cultures of the peripheral blood, skin fibroblast, or other tissues. Although true hermaphroditism was suspected, exploration of the bilateral gonadal structures failed to detect the presence of ovarian structures, either grossly or microscopically. Furthermore, exploratory laparotomy revealed no Mullerian structures. The difficulty of early diagnosis of XX males in infancy is emphasized.", "PMID": 975596}
{"id": "pubmed23n0032_10364", "title": "Multiple none-erupting teeth, maxillo-zygomatical hypoplasia and other congenital defects: An autosomal recessive disorder.", "content": "A new syndrome is described, affecting four persons in one family; it includes multiple non-erupting teeth with cementum defects, hypoplasia of the alveolar process and the maxillo-zygomatical region, genua valga and ear deformities. An autosomal recessive trait could be ascertained.", "contents": "Multiple none-erupting teeth, maxillo-zygomatical hypoplasia and other congenital defects: An autosomal recessive disorder. A new syndrome is described, affecting four persons in one family; it includes multiple non-erupting teeth with cementum defects, hypoplasia of the alveolar process and the maxillo-zygomatical region, genua valga and ear deformities. An autosomal recessive trait could be ascertained.", "PMID": 975598}
{"id": "pubmed23n0032_10365", "title": "A familial syndrome of cranial, facial, oral and limb anomalies.", "content": "A family is described in which two male infants have microcephaly, abnormal ears, anti-mongoloid slant, small mouth, cleft palate, flexed overlapping fingers with syndactyly of digits three and four, syndactyly of the second to the fifth toes, and normal karyotype. This seems to be a new syndrome.", "contents": "A familial syndrome of cranial, facial, oral and limb anomalies. A family is described in which two male infants have microcephaly, abnormal ears, anti-mongoloid slant, small mouth, cleft palate, flexed overlapping fingers with syndactyly of digits three and four, syndactyly of the second to the fifth toes, and normal karyotype. This seems to be a new syndrome.", "PMID": 975599}
{"id": "pubmed23n0032_10366", "title": "Benzo-pyrones: their selective injury to rabbit vascular endothelium.", "content": "1. Three benzo-pyrones were tested in two types of high protein oedema. 2. In general, they prevented increases in permeability in superficial venules. 3. In the absence of an injurious stimulus they caused injury to the superficial capillaries and deep venules. 4. The injurious results may be due to a direct effect on the vessel walls or to an increase in intravascular pressure and flow. This would enhance leakage from already existing defects. 5. Despite the minor injuries the effectiveness of these drugs is so great that a net removal of protein by proteolysis from the tissues occurs, thus reducing the oedema. 6. The study contributes further to an understanding of the mode of action of the benzo-pyrones.", "contents": "Benzo-pyrones: their selective injury to rabbit vascular endothelium. 1. Three benzo-pyrones were tested in two types of high protein oedema. 2. In general, they prevented increases in permeability in superficial venules. 3. In the absence of an injurious stimulus they caused injury to the superficial capillaries and deep venules. 4. The injurious results may be due to a direct effect on the vessel walls or to an increase in intravascular pressure and flow. This would enhance leakage from already existing defects. 5. Despite the minor injuries the effectiveness of these drugs is so great that a net removal of protein by proteolysis from the tissues occurs, thus reducing the oedema. 6. The study contributes further to an understanding of the mode of action of the benzo-pyrones.", "PMID": 975609}
{"id": "pubmed23n0032_10367", "title": "The inhibitory response of the rabbit anococcygeus muscle.", "content": "1. Strips of rabbit anococcygeus muscle were submaximally contracted by the addition of phenylephrine to the bath fluid. 2. Acetylcholine, dimethylphenylpiperazinium and electrical field stimulation caused relaxation. Relaxation induced by acetylcholine and field stimulation was blocked by tetrodotoxin but only the acetylcholine-induced response was inhibited by lignocaine, tetraethylammonium and hexamethonium. The responses to both acetylcholine and field stimulation were resistant to atropine, practolol, and sotalol. 3. Relatively high concentrations of ATP caused relaxation. 4. Histological investigation did not show any cells resembling autonomic ganglion cells. 5. It is suggested that both acetylcholine and field stimulation release an inhibitory transmitter from nerve endings and that the release by acetylcholine is mediated through nicotinic receptor stimulation.", "contents": "The inhibitory response of the rabbit anococcygeus muscle. 1. Strips of rabbit anococcygeus muscle were submaximally contracted by the addition of phenylephrine to the bath fluid. 2. Acetylcholine, dimethylphenylpiperazinium and electrical field stimulation caused relaxation. Relaxation induced by acetylcholine and field stimulation was blocked by tetrodotoxin but only the acetylcholine-induced response was inhibited by lignocaine, tetraethylammonium and hexamethonium. The responses to both acetylcholine and field stimulation were resistant to atropine, practolol, and sotalol. 3. Relatively high concentrations of ATP caused relaxation. 4. Histological investigation did not show any cells resembling autonomic ganglion cells. 5. It is suggested that both acetylcholine and field stimulation release an inhibitory transmitter from nerve endings and that the release by acetylcholine is mediated through nicotinic receptor stimulation.", "PMID": 975610}
{"id": "pubmed23n0032_10368", "title": "Effects of calcitonin on the secretion of pancreatic juice induced by dopamine, secretin and pancreozymin.", "content": "1. Effects of calcitonin on dopamine-, secretin- and pancreozymin-induced pancreatic secretion were investigated in the isolated blood-perfused canine pancreas. 2. The volume of pancreatic secretion induced by pancreozymin given intra-arterially (i.a.) was decreased by an i.a. infusion of 1 u/min of calcitonin, but that induced by dopamine or secretin given i.a. was not affected by calcitonin treatment. 3. Amylase concentration in pancreatic juice either in spontaneous secretion in the resting state or in that of stimulated secretion by pancreozymin was decreased approximately 30% by calcitonin treatment, but amylase concentration in pancreatic juice induced by dopamine or secretin was not affected by calcitonin treatment. 4. Calcitonin had no effect on bicarbonate concentration in pancreatic juice stimulated by these secretagogues. 5. Calcium concentration in pancreatic juice in the resting state was reduced about 36% by calcitonin treatment. Calcitonin caused a decrease in a calcium concentration in the pancreozymin-induced secretion, but did not cause any change in the dopamine- or secretin-induced one. 6. These results suggest that calcitonin may affect the secretory mechanism of the acinar cells but not that of the ductular cells, and that the acinar cells are active even in the resting state.", "contents": "Effects of calcitonin on the secretion of pancreatic juice induced by dopamine, secretin and pancreozymin. 1. Effects of calcitonin on dopamine-, secretin- and pancreozymin-induced pancreatic secretion were investigated in the isolated blood-perfused canine pancreas. 2. The volume of pancreatic secretion induced by pancreozymin given intra-arterially (i.a.) was decreased by an i.a. infusion of 1 u/min of calcitonin, but that induced by dopamine or secretin given i.a. was not affected by calcitonin treatment. 3. Amylase concentration in pancreatic juice either in spontaneous secretion in the resting state or in that of stimulated secretion by pancreozymin was decreased approximately 30% by calcitonin treatment, but amylase concentration in pancreatic juice induced by dopamine or secretin was not affected by calcitonin treatment. 4. Calcitonin had no effect on bicarbonate concentration in pancreatic juice stimulated by these secretagogues. 5. Calcium concentration in pancreatic juice in the resting state was reduced about 36% by calcitonin treatment. Calcitonin caused a decrease in a calcium concentration in the pancreozymin-induced secretion, but did not cause any change in the dopamine- or secretin-induced one. 6. These results suggest that calcitonin may affect the secretory mechanism of the acinar cells but not that of the ductular cells, and that the acinar cells are active even in the resting state.", "PMID": 975611}
{"id": "pubmed23n0032_10369", "title": "Effect of secretin on pacemaker activity and contractility in the isolated blood-perfused atrium of the dog.", "content": "1. The effects of secretin on inotropic and chronotropic activity were investigated in nine isolated canine atrium preparations which were suspended in a bath and perfused with arterial blood from a carotid artery of a heparinized donor dog. 2. Secretin administered into the cannulated sinus node artery in a dose range of 0-1-10 units produced a dose-related positive inotropic and a biphasic chronotropic effect. 3. The positive chronotropic and inotropic responses to secretin were not suppressed by treatment with alprenolol in doses which blocked responses to noradrenaline. 4. The negative chronotropic response to secretin was not blocked by atropine in doses which blocked the response to acetylcholine. 5. After treatment with glucagon, secretin produced dose-related negative chronotropic and a positive inotropic effects. Thus glucagon may antagonize the positive chronotropic effect of secretin. 6. From these results, it is concluded that secretin has a direct effect on atrial rate and contractility.", "contents": "Effect of secretin on pacemaker activity and contractility in the isolated blood-perfused atrium of the dog. 1. The effects of secretin on inotropic and chronotropic activity were investigated in nine isolated canine atrium preparations which were suspended in a bath and perfused with arterial blood from a carotid artery of a heparinized donor dog. 2. Secretin administered into the cannulated sinus node artery in a dose range of 0-1-10 units produced a dose-related positive inotropic and a biphasic chronotropic effect. 3. The positive chronotropic and inotropic responses to secretin were not suppressed by treatment with alprenolol in doses which blocked responses to noradrenaline. 4. The negative chronotropic response to secretin was not blocked by atropine in doses which blocked the response to acetylcholine. 5. After treatment with glucagon, secretin produced dose-related negative chronotropic and a positive inotropic effects. Thus glucagon may antagonize the positive chronotropic effect of secretin. 6. From these results, it is concluded that secretin has a direct effect on atrial rate and contractility.", "PMID": 975612}
{"id": "pubmed23n0032_10370", "title": "Differing effects of the vasodilator drugs, prazosin and diazoxide on plasma renin activity in the dog.", "content": "1. The effects of intravenous (i.v.) administration of the vasodilator drugs prazosin or diazoxide on blood pressure and plasma renin activity were evaluated in the anaesthetized dog. 2. Prazosin and diazoxide both induced a rapid reduction in the mean arterial pressure to 73% and 75% of control values respectively. 3. Prazosin lowered plasma renin activity to 62% (P less than 0-025) of the control value whereas diazoxide raised plasma renin activity to 178% (P less than 0.05) of the control value. 4. The combination of vasodilatation and low renin activity observed following the administration of prazosin is unique, and may have clinical significance if these factors reduce the vascular complications of hypertension.", "contents": "Differing effects of the vasodilator drugs, prazosin and diazoxide on plasma renin activity in the dog. 1. The effects of intravenous (i.v.) administration of the vasodilator drugs prazosin or diazoxide on blood pressure and plasma renin activity were evaluated in the anaesthetized dog. 2. Prazosin and diazoxide both induced a rapid reduction in the mean arterial pressure to 73% and 75% of control values respectively. 3. Prazosin lowered plasma renin activity to 62% (P less than 0-025) of the control value whereas diazoxide raised plasma renin activity to 178% (P less than 0.05) of the control value. 4. The combination of vasodilatation and low renin activity observed following the administration of prazosin is unique, and may have clinical significance if these factors reduce the vascular complications of hypertension.", "PMID": 975613}
{"id": "pubmed23n0032_10371", "title": "Renal vasodilation in response to coronary artery ligation in the dog.", "content": "1. The effects of coronary artery ligation on renal vascular resistance were determined with and without pretreatment with methylprednisolone succinate or atropine in anaesthetized dogs. 2. With coronary artery ligation alone renal blood flow is maintained constant in spite of a reduction in arterial blood pressure, indicating renal vasodilation. 3. Pretreatment with methylprednisolone succinate prevents the fall in arterial blood pressure. 4. After pretreatment with atropine, renal blood flow falls in response to the reduction in arterial blood pressure, suggesting inhibition of cholinergic renal vasodilatation.", "contents": "Renal vasodilation in response to coronary artery ligation in the dog. 1. The effects of coronary artery ligation on renal vascular resistance were determined with and without pretreatment with methylprednisolone succinate or atropine in anaesthetized dogs. 2. With coronary artery ligation alone renal blood flow is maintained constant in spite of a reduction in arterial blood pressure, indicating renal vasodilation. 3. Pretreatment with methylprednisolone succinate prevents the fall in arterial blood pressure. 4. After pretreatment with atropine, renal blood flow falls in response to the reduction in arterial blood pressure, suggesting inhibition of cholinergic renal vasodilatation.", "PMID": 975614}
{"id": "pubmed23n0032_10372", "title": "A diminution of enzyme release in the anoxic guinea-pig heart observed after cardoiplegic treatment.", "content": "1. Under anaerobic conditions there was an increase in the release of malate dehydrogenase, lactate dehydrogenase and creatine phosphokinase from isolated perfused guinea-pig hearts. 2. The increased enzyme releases were significantly reduced by pretreatment with cardioplegic solution. 3. This finding suggests a relationship between myocardial energy metabolism and enzyme release or retention.", "contents": "A diminution of enzyme release in the anoxic guinea-pig heart observed after cardoiplegic treatment. 1. Under anaerobic conditions there was an increase in the release of malate dehydrogenase, lactate dehydrogenase and creatine phosphokinase from isolated perfused guinea-pig hearts. 2. The increased enzyme releases were significantly reduced by pretreatment with cardioplegic solution. 3. This finding suggests a relationship between myocardial energy metabolism and enzyme release or retention.", "PMID": 975615}
{"id": "pubmed23n0032_10373", "title": "Tachycardia in spontaneously hypertensive and normotensive rats after fusaric acid and bupicamide.", "content": "1. The effects of the dopamine-beta-hydroxylase inhibitors bupicamide, fusaric acid, FLA-63 and U-14,624 on blood pressure and heart rate of spontaneously hypertensive rats were examined. 2. Bupicamide and fusaric acid caused marked tachycardia whereas FLA-63 and U-14,624 caused modest bradycardia; all drugs decreased blood pressure. 3. In normotensive rats, fusaric acid caused the same degree of tachycardia as in spontaneously hypertensive rats, but blood pressure was only slightly reduced. 4. Tachycardia after fusaric acid was not due to increased sympathetic activity or decreased parasympathetic activity but required intact catecholamine stores. 5. It is concluded that fusaric acid causes tachycardia by releasing catecholamines indirectly and that a metabolite of fusaric acid is also involved.", "contents": "Tachycardia in spontaneously hypertensive and normotensive rats after fusaric acid and bupicamide. 1. The effects of the dopamine-beta-hydroxylase inhibitors bupicamide, fusaric acid, FLA-63 and U-14,624 on blood pressure and heart rate of spontaneously hypertensive rats were examined. 2. Bupicamide and fusaric acid caused marked tachycardia whereas FLA-63 and U-14,624 caused modest bradycardia; all drugs decreased blood pressure. 3. In normotensive rats, fusaric acid caused the same degree of tachycardia as in spontaneously hypertensive rats, but blood pressure was only slightly reduced. 4. Tachycardia after fusaric acid was not due to increased sympathetic activity or decreased parasympathetic activity but required intact catecholamine stores. 5. It is concluded that fusaric acid causes tachycardia by releasing catecholamines indirectly and that a metabolite of fusaric acid is also involved.", "PMID": 975616}
{"id": "pubmed23n0032_10374", "title": "The elimination of phenytoin in man.", "content": "1. Plasma phenytoin (diphenylhydantoin) levels after different drug doses were correlated with urinary 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) excretions in four subjects. 2. In three of four subjects the proportion of the phenytoin dose that was excreted as p-HPPH. In the fourth, p-HPPH output remained proportionate to dose of phenytoin until elimination of the drug fell below its input. 3. Plasma p-HPPH levels were measured in two subjects; the data suggested that the renal excretion of p-HPPH was not rate-limited. 4. In three of four subjects, there was the possibility that alternative pathways for eliminating phenytoin may have developed as drug doses increased and the capacity for forming p-HPPH became saturated. 5. Overall phenytoin elimination appeared to approach saturation at concentrations of the drug encountered therapeutically. When Michaelis-Menten kinetics were applied to data for phenytoin elimination in twenty-one adults and fifteen children, the mean apparent Km value for the adults corresponded to a plasma drug concentration of 5-8 mug/ml, and in the children to 5-3 mug/ml. The mean Vmax values in the two groups were, respectively 8-1 mg/kg per day and 12-5 mg/kg per day.", "contents": "The elimination of phenytoin in man. 1. Plasma phenytoin (diphenylhydantoin) levels after different drug doses were correlated with urinary 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) excretions in four subjects. 2. In three of four subjects the proportion of the phenytoin dose that was excreted as p-HPPH. In the fourth, p-HPPH output remained proportionate to dose of phenytoin until elimination of the drug fell below its input. 3. Plasma p-HPPH levels were measured in two subjects; the data suggested that the renal excretion of p-HPPH was not rate-limited. 4. In three of four subjects, there was the possibility that alternative pathways for eliminating phenytoin may have developed as drug doses increased and the capacity for forming p-HPPH became saturated. 5. Overall phenytoin elimination appeared to approach saturation at concentrations of the drug encountered therapeutically. When Michaelis-Menten kinetics were applied to data for phenytoin elimination in twenty-one adults and fifteen children, the mean apparent Km value for the adults corresponded to a plasma drug concentration of 5-8 mug/ml, and in the children to 5-3 mug/ml. The mean Vmax values in the two groups were, respectively 8-1 mg/kg per day and 12-5 mg/kg per day.", "PMID": 975617}
{"id": "pubmed23n0032_10375", "title": "Plasma propranolol concentration in patients with angina and acute myocardial infarction.", "content": "1. Plasma levels of propranolol were measured fluorometrically in patients with angina pectoris and in patients admitted to the Coronary Care Unit with acute myocardial infarction. 2. In thirty patients with stable angina pectoris, plasma propranolol levels varied almost linearly with doses between 10 and 120 mg during 6-hourly chronic oral administration. Plasma levels greater than 100 ng/ml produced 70-80% reduction in the tachycardia induced by strenous exercise on a treadmill. 3. In nineteen patients with acute myocardial infarction given oral propranolol, 20 mg 6-hourly, peak as well as trough plasma levels of the drug increased progressively but remained below 100 ng/ml in all except two patients during the first 24 h after their admission to the Coronary Care Unit. 4. The data suggest that the use of low and fixed doses of propranolol may not produce adequate plasma levels or significant beta-adrenoceptor blockade in the early stages of acute myocardial infarction in man.", "contents": "Plasma propranolol concentration in patients with angina and acute myocardial infarction. 1. Plasma levels of propranolol were measured fluorometrically in patients with angina pectoris and in patients admitted to the Coronary Care Unit with acute myocardial infarction. 2. In thirty patients with stable angina pectoris, plasma propranolol levels varied almost linearly with doses between 10 and 120 mg during 6-hourly chronic oral administration. Plasma levels greater than 100 ng/ml produced 70-80% reduction in the tachycardia induced by strenous exercise on a treadmill. 3. In nineteen patients with acute myocardial infarction given oral propranolol, 20 mg 6-hourly, peak as well as trough plasma levels of the drug increased progressively but remained below 100 ng/ml in all except two patients during the first 24 h after their admission to the Coronary Care Unit. 4. The data suggest that the use of low and fixed doses of propranolol may not produce adequate plasma levels or significant beta-adrenoceptor blockade in the early stages of acute myocardial infarction in man.", "PMID": 975619}
{"id": "pubmed23n0032_10376", "title": "The specificity of bile salts in the intestinal absorption of micellar cholesterol in the rat.", "content": "1. Two aspects of cholesterol absorption; (a) the importance of solubilization and (b) the effects of different bile salts on the mucosal metabolism and lymphatic output of cholesterol, have been investigated using two different in vivo techniques. 2. Bile diverted lymph fistula rats were infused intraduodenally at a steady rate with a constant lipid mixture containing labelled cholesterol, labelled oleic acid and mono-olein. The lipids were completely solubilized in either bile salts or a non-toxic non-ionic detergent, Pluronic F68. Labelled fatty acid was efficiently absorbed from either micellar infusate but virtually no labelled cholesterol appeared in the lymph in the absence of bile salts. 3. Short-term perfusions of the intestine of anaesthetized rats with the same micellar perfusates as above showed approximately 20% of the labelled cholesterol in the mucosa after 30 min perfusion with the bile salt micellar solutions. When the non-ionic micelles were used virtually no isotopic cholesterol left the lumen. 4. Mucosal uptake of labelled cholesterol was linearly dependent on the concentration of solubilized cholesterol in the perfusate and was not dependent on the bile salt concentration. 5. After 30 min the total amount of perfused isotopic cholesterol was recovered from either the lumen or the mucosa, but some fatty acid appeared to have been transported away from the mucosa by this time. 6. The initial rate of mucosal uptake of labelled cholesterol was similar from micellar perfusates using either taurocholate, taurodeoxycholate or taurofusidate. In contrast, after 8 h of infusion, lymphatic output of labelled cholesterol was markedly greater with taurocholate. 7. The increased lymph output with taurocholate was associated with an increase in the esterified fraction of both labelled and unlabelled cholesterol. Fatty acid was absorbed and esterified equally from all three types of perfusate. 8. These results suggested that for the first step in cholesterol absorption, viz. uptake from the lumen, solubilization by a planar detergent was essential. After uptake, the more rapid transfer of cholesterol to lymph in the presence of trihydroxybile acids appeared to be related to a more efficient esterification of cholesterol, but not to a more efficient resynthesis of triglyceride, the other major component of lymph chylomicrons.", "contents": "The specificity of bile salts in the intestinal absorption of micellar cholesterol in the rat. 1. Two aspects of cholesterol absorption; (a) the importance of solubilization and (b) the effects of different bile salts on the mucosal metabolism and lymphatic output of cholesterol, have been investigated using two different in vivo techniques. 2. Bile diverted lymph fistula rats were infused intraduodenally at a steady rate with a constant lipid mixture containing labelled cholesterol, labelled oleic acid and mono-olein. The lipids were completely solubilized in either bile salts or a non-toxic non-ionic detergent, Pluronic F68. Labelled fatty acid was efficiently absorbed from either micellar infusate but virtually no labelled cholesterol appeared in the lymph in the absence of bile salts. 3. Short-term perfusions of the intestine of anaesthetized rats with the same micellar perfusates as above showed approximately 20% of the labelled cholesterol in the mucosa after 30 min perfusion with the bile salt micellar solutions. When the non-ionic micelles were used virtually no isotopic cholesterol left the lumen. 4. Mucosal uptake of labelled cholesterol was linearly dependent on the concentration of solubilized cholesterol in the perfusate and was not dependent on the bile salt concentration. 5. After 30 min the total amount of perfused isotopic cholesterol was recovered from either the lumen or the mucosa, but some fatty acid appeared to have been transported away from the mucosa by this time. 6. The initial rate of mucosal uptake of labelled cholesterol was similar from micellar perfusates using either taurocholate, taurodeoxycholate or taurofusidate. In contrast, after 8 h of infusion, lymphatic output of labelled cholesterol was markedly greater with taurocholate. 7. The increased lymph output with taurocholate was associated with an increase in the esterified fraction of both labelled and unlabelled cholesterol. Fatty acid was absorbed and esterified equally from all three types of perfusate. 8. These results suggested that for the first step in cholesterol absorption, viz. uptake from the lumen, solubilization by a planar detergent was essential. After uptake, the more rapid transfer of cholesterol to lymph in the presence of trihydroxybile acids appeared to be related to a more efficient esterification of cholesterol, but not to a more efficient resynthesis of triglyceride, the other major component of lymph chylomicrons.", "PMID": 975620}
{"id": "pubmed23n0032_10377", "title": "The effects of hydrocortisone on responses to and extraneuronal uptake of (-)-isoprenaline in cat and guinea-pig atria.", "content": "1. (-)-Isoprenaline-induced positive inotropic effects were assessed in driven left atria and positive chronotropic effects in spontaneously beating right atria from guinea-pigs and cats. 2. Hydrocortisone produced dose dependent shifts to the left in cumulative concentration-effect curves to (-)-isoprenaline in left and right atrial preparations from the cat. 3. Maximal potentiations were produced by 160 muM and 40 muM hydrocortisone in cat left and right atria respectively. 4. No such effects were seen in similar preparations from the guinea-pig even in the presence of concentrations of hydrocortisone as high as 400 muM. 5. Hydrocortisone inhibited the extraneuronal uptake of [7 - 3H](-)-isoprenaline in a concentration dependent manner in both left and right atria of the cat, but not the guinea-pig. 6. These results are consistent with the idea that hydrocortisone potentiates responses to (-)-isoprenaline in cat atria by inhibition of the extraneuronal uptake process.", "contents": "The effects of hydrocortisone on responses to and extraneuronal uptake of (-)-isoprenaline in cat and guinea-pig atria. 1. (-)-Isoprenaline-induced positive inotropic effects were assessed in driven left atria and positive chronotropic effects in spontaneously beating right atria from guinea-pigs and cats. 2. Hydrocortisone produced dose dependent shifts to the left in cumulative concentration-effect curves to (-)-isoprenaline in left and right atrial preparations from the cat. 3. Maximal potentiations were produced by 160 muM and 40 muM hydrocortisone in cat left and right atria respectively. 4. No such effects were seen in similar preparations from the guinea-pig even in the presence of concentrations of hydrocortisone as high as 400 muM. 5. Hydrocortisone inhibited the extraneuronal uptake of [7 - 3H](-)-isoprenaline in a concentration dependent manner in both left and right atria of the cat, but not the guinea-pig. 6. These results are consistent with the idea that hydrocortisone potentiates responses to (-)-isoprenaline in cat atria by inhibition of the extraneuronal uptake process.", "PMID": 975618}
{"id": "pubmed23n0032_10378", "title": "The effects of 3-hydrazine-6[N,N-bis(2-hydroxyethyl)-amino]-pyridazine dihydrochloride (L6150) on the general and cardiac haemodynamics of the intact dog.", "content": "1. The effects of 3-hydrazine-6[N,N-bis(2-hydroxyethyl)amino]-pyridazine dihydrochloride (L6150) were studied in intact anaesthetized dogs. 2. In a dose of 1 mg/kg/(i.v.) the drug increased respiration rate, cardiac output, and left and right ventricular work. 3. It decreased femoral pressure and systemic and total pulmonary resistance. 4. It increased coronary flow and coronary sinus O2 content. 5 The drug also increased whole body glucose, lactate, pyruvate and NEFA values.", "contents": "The effects of 3-hydrazine-6[N,N-bis(2-hydroxyethyl)-amino]-pyridazine dihydrochloride (L6150) on the general and cardiac haemodynamics of the intact dog. 1. The effects of 3-hydrazine-6[N,N-bis(2-hydroxyethyl)amino]-pyridazine dihydrochloride (L6150) were studied in intact anaesthetized dogs. 2. In a dose of 1 mg/kg/(i.v.) the drug increased respiration rate, cardiac output, and left and right ventricular work. 3. It decreased femoral pressure and systemic and total pulmonary resistance. 4. It increased coronary flow and coronary sinus O2 content. 5 The drug also increased whole body glucose, lactate, pyruvate and NEFA values.", "PMID": 975622}
{"id": "pubmed23n0032_10379", "title": "A study of the actions of histamine on the isolated rat heart.", "content": "1. The effects of histamine on cardiac force, heart rate and coronary perfusion pressure were studied in the isolated rat heart, using the Langendorff perfused heart preparation. 2. Single injections of histamine induced dose-dependent decreases in contractile amplitude, heart rate and coronary perfusion pressure. 3. Perfusions of metiamide (above 1 x 10(-4) m) had a depressant effect on contractile force and heart rate, whereas diphenhydramine (4 x 10(-6) m) reduced only the heart rate. Both agents caused a fall in coronary perfusion pressure. 4. The negative inotropic and chronotropic effects of histamine on the isolated rat heart were not significantly influenced by either metiamide of diphenhydramine, or a combination of these drugs. However, the fall in coronary perfusion pressure induced by injections of histamine was significantly antagonized by metiamide or diphenhydramine. 5. These results suggest that the effects of histamine on the isolated rat heart may not be due entirely to stimulation of H1- or H2-receptors on the cardiac muscle cells. Evidence is presented for the existence of histamine H1- and H2-receptors in the coronary vessels.", "contents": "A study of the actions of histamine on the isolated rat heart. 1. The effects of histamine on cardiac force, heart rate and coronary perfusion pressure were studied in the isolated rat heart, using the Langendorff perfused heart preparation. 2. Single injections of histamine induced dose-dependent decreases in contractile amplitude, heart rate and coronary perfusion pressure. 3. Perfusions of metiamide (above 1 x 10(-4) m) had a depressant effect on contractile force and heart rate, whereas diphenhydramine (4 x 10(-6) m) reduced only the heart rate. Both agents caused a fall in coronary perfusion pressure. 4. The negative inotropic and chronotropic effects of histamine on the isolated rat heart were not significantly influenced by either metiamide of diphenhydramine, or a combination of these drugs. However, the fall in coronary perfusion pressure induced by injections of histamine was significantly antagonized by metiamide or diphenhydramine. 5. These results suggest that the effects of histamine on the isolated rat heart may not be due entirely to stimulation of H1- or H2-receptors on the cardiac muscle cells. Evidence is presented for the existence of histamine H1- and H2-receptors in the coronary vessels.", "PMID": 975624}
{"id": "pubmed23n0032_10380", "title": "Increase in myocardial digoxin content associated with circulatory volume overload in the dog.", "content": "1. Tritiated (12alpha-3H) digoxin (0-05 mg/kg body weight) was administered intravenously to conscious dogs with circulatory volume overload induced by previous creation of aorto-caval fistulae. Dogs were killed after 5 min, 1, or 4 h, and the myocardium sampled. Digoxin was extracted and counted and results compared to those in normal dogs. 2. At each time, myocardial digoxin concentration of all cardiac chambers in test dogs was greater than normal. Plasma digoxin concentration measured 5 min after administration was greater in dogs with fistulae but the subsequent levels were not different. 3. Anaesthetized and open-chest dogs with fistulae studied 5 min after digoxin administration had greater myocardial concentrations than similarly studied normal dogs. Although myocardial concentrations of digoxin were higher in anaesthetized than in conscious dogs the group with fistulae had higher values than did the normal group, as was the case for unanaesthetized dogs. 4. The basis for the effect of fistula is probably multifactorial. Diminised peripheral blood flow and peripheral digoxin delivery and uptake, resulting initially in higher digoxin levels in plasma perfusing the myocardium, may play a role. Increased myocardial mechanical and metabolic activity almost certainly are important. Cardiac hypertrophy, cardiac failure per se and plasma electrolyte changes are probably not. 5. The results are consistent with previously demonstrated reduced digitalis tolerance in the dog with circulatory volume overload.", "contents": "Increase in myocardial digoxin content associated with circulatory volume overload in the dog. 1. Tritiated (12alpha-3H) digoxin (0-05 mg/kg body weight) was administered intravenously to conscious dogs with circulatory volume overload induced by previous creation of aorto-caval fistulae. Dogs were killed after 5 min, 1, or 4 h, and the myocardium sampled. Digoxin was extracted and counted and results compared to those in normal dogs. 2. At each time, myocardial digoxin concentration of all cardiac chambers in test dogs was greater than normal. Plasma digoxin concentration measured 5 min after administration was greater in dogs with fistulae but the subsequent levels were not different. 3. Anaesthetized and open-chest dogs with fistulae studied 5 min after digoxin administration had greater myocardial concentrations than similarly studied normal dogs. Although myocardial concentrations of digoxin were higher in anaesthetized than in conscious dogs the group with fistulae had higher values than did the normal group, as was the case for unanaesthetized dogs. 4. The basis for the effect of fistula is probably multifactorial. Diminised peripheral blood flow and peripheral digoxin delivery and uptake, resulting initially in higher digoxin levels in plasma perfusing the myocardium, may play a role. Increased myocardial mechanical and metabolic activity almost certainly are important. Cardiac hypertrophy, cardiac failure per se and plasma electrolyte changes are probably not. 5. The results are consistent with previously demonstrated reduced digitalis tolerance in the dog with circulatory volume overload.", "PMID": 975623}
{"id": "pubmed23n0032_10381", "title": "On the role of serotonin in the pathogenesis of pulmonary hypertension induced by anorectic drugs; an experimental study in the isolated perfused rat lung, II. Fenfluramine, mazindol, mefenorex, phentermine and R 800.", "content": "1. The influence of the anorectic drugs fenfluramine, mazindol, mefenorex, phentermine and R 800, an experimental compound, on pulmonary vascular resistance has been studied in the isolated, perfused rat lung. 2. R 800 caused a strong vasoconstriction, which was not antagonized by methysergide of phentolamine; the other drugs listed did not alter vascular resistance. 3. Mazindol and phentermine significantly prolonged the vasoconstrictive effect of serotonin due to inhibition of its metabolic breakdown. 4. Although fenfluramine inhibited serotonin metabolism it also prevented the vasoconstrictive effect of serotonin, due to its ability to act as a serotonin antagonist. 5. Mefenorex did not affect pulmonary vascular resistance, either directly or indirectly via a serotoninergic mechanism.", "contents": "On the role of serotonin in the pathogenesis of pulmonary hypertension induced by anorectic drugs; an experimental study in the isolated perfused rat lung, II. Fenfluramine, mazindol, mefenorex, phentermine and R 800. 1. The influence of the anorectic drugs fenfluramine, mazindol, mefenorex, phentermine and R 800, an experimental compound, on pulmonary vascular resistance has been studied in the isolated, perfused rat lung. 2. R 800 caused a strong vasoconstriction, which was not antagonized by methysergide of phentolamine; the other drugs listed did not alter vascular resistance. 3. Mazindol and phentermine significantly prolonged the vasoconstrictive effect of serotonin due to inhibition of its metabolic breakdown. 4. Although fenfluramine inhibited serotonin metabolism it also prevented the vasoconstrictive effect of serotonin, due to its ability to act as a serotonin antagonist. 5. Mefenorex did not affect pulmonary vascular resistance, either directly or indirectly via a serotoninergic mechanism.", "PMID": 975621}
{"id": "pubmed23n0032_10382", "title": "Hepatic sinusoidal responses to intraportal injections of phenylephrine and isoprenaline in the rat.", "content": "1. Specific alpha- and beta-adrenoreceptor agonists, phenylephrine and isoprenaline, were injected intraportally into the intact rat liver under direct microscopic observation by an in vivo transillumination technique. 2. The diameter of a hepatic sinusoid and the intra-sinusoidal erythrocyte velocity were quantitatively measured, and the sinusoidal volume flow was calculated from these two parameters. 3. Results show that phenylephrine causes a sinusoidal constriction and an increased sinusoidal blood flow, whereas isoprenaline causes the opposite effects on the sinusoids. 4. All the sinusoidal responses to phenylephrine and isoprenaline were dose-dependent and were possibly related to the direct effect of these drugs on the sinusoids.", "contents": "Hepatic sinusoidal responses to intraportal injections of phenylephrine and isoprenaline in the rat. 1. Specific alpha- and beta-adrenoreceptor agonists, phenylephrine and isoprenaline, were injected intraportally into the intact rat liver under direct microscopic observation by an in vivo transillumination technique. 2. The diameter of a hepatic sinusoid and the intra-sinusoidal erythrocyte velocity were quantitatively measured, and the sinusoidal volume flow was calculated from these two parameters. 3. Results show that phenylephrine causes a sinusoidal constriction and an increased sinusoidal blood flow, whereas isoprenaline causes the opposite effects on the sinusoids. 4. All the sinusoidal responses to phenylephrine and isoprenaline were dose-dependent and were possibly related to the direct effect of these drugs on the sinusoids.", "PMID": 975627}
{"id": "pubmed23n0032_10383", "title": "Adrenoceptor and cholinoceptor mediated effects in the isolated urethra of cat and guinea-pig.", "content": "1. Preparations of isolated bladder-urethral junction from cats and guinea-pigs were suspended in Krebs solution (37 degrees C) which was bubbled with carbogen. The urethral lumen was perfused at a very low rate (1-3 ml/h) with Krebs solution. Resistance to flow and changes in longitudinal tension (initial setting 0-5 g) were recorded. In additional experiments, tension changes in urethral circular muscle preparations were registered. 2. The urethral preparations had a basal resistance to flow within the range 5-20 cm H2O. They had a spontaneous contractile activity that was unaffected by atropine 0-1 mug/ml, phenoxybenzamine 0-1 mug/ml, and tetrodotoxin 0-1-0-5 mug/ml, suggesting it was of myogenic origin. 3. The basal characteristics and the drug-induced changes in the perfused urethras were independent of whether the longitudinal tension was recorded isometrically or isotonically, or whether the perfusion was made retrograde or antegrade. Passive changes in longitudinal tension between 0-5 and 2-0 g did not affect the resistance to flow through the urethra. 4. Adrenaline and noradrenaline, 0-01-1 mug/ml, increased longitudinal tension and resistance to flow in the perfused preparations. The effects, which were sustained and concentration-related, were blocked or reversed into inhibition in the presence of phenoxybenzamine, 0-1 mug/ml. This finding suggests that the stimulatory effects were mediated via alpha-receptors. 5. Isoprenaline, 0-001-0-005 mug/ml, relaxed and inhibited the activity in the cat urethral smooth muscle and decreased the resistance to flow both under basal conditions and when the urethra was contracted by noradrenaline or acetylcholine. The inhibitory effects were blocked by propranolol, 0-1 mug/ml, suggesting that they were mediated via beta-receptors. In guinea-pig preparations, no effect of isoprenaline was observed. 6. Acetylcholine, 0-02-0-5 mug/ml, increased the longitudinal tension and the resistance to flow through the urethras. The latter effect was less pronounced than that produced by noradrenaline, especially in the guinea-pig preparations. Noradrenaline was also more effective than ecetylcholine in contracting the circular muscle of the urethra. The effect of acetylcholine was blocked by atropine, 0-1 mug/ml, suggesting that the contractions were mediated through muscarinic cholinoceptors.", "contents": "Adrenoceptor and cholinoceptor mediated effects in the isolated urethra of cat and guinea-pig. 1. Preparations of isolated bladder-urethral junction from cats and guinea-pigs were suspended in Krebs solution (37 degrees C) which was bubbled with carbogen. The urethral lumen was perfused at a very low rate (1-3 ml/h) with Krebs solution. Resistance to flow and changes in longitudinal tension (initial setting 0-5 g) were recorded. In additional experiments, tension changes in urethral circular muscle preparations were registered. 2. The urethral preparations had a basal resistance to flow within the range 5-20 cm H2O. They had a spontaneous contractile activity that was unaffected by atropine 0-1 mug/ml, phenoxybenzamine 0-1 mug/ml, and tetrodotoxin 0-1-0-5 mug/ml, suggesting it was of myogenic origin. 3. The basal characteristics and the drug-induced changes in the perfused urethras were independent of whether the longitudinal tension was recorded isometrically or isotonically, or whether the perfusion was made retrograde or antegrade. Passive changes in longitudinal tension between 0-5 and 2-0 g did not affect the resistance to flow through the urethra. 4. Adrenaline and noradrenaline, 0-01-1 mug/ml, increased longitudinal tension and resistance to flow in the perfused preparations. The effects, which were sustained and concentration-related, were blocked or reversed into inhibition in the presence of phenoxybenzamine, 0-1 mug/ml. This finding suggests that the stimulatory effects were mediated via alpha-receptors. 5. Isoprenaline, 0-001-0-005 mug/ml, relaxed and inhibited the activity in the cat urethral smooth muscle and decreased the resistance to flow both under basal conditions and when the urethra was contracted by noradrenaline or acetylcholine. The inhibitory effects were blocked by propranolol, 0-1 mug/ml, suggesting that they were mediated via beta-receptors. In guinea-pig preparations, no effect of isoprenaline was observed. 6. Acetylcholine, 0-02-0-5 mug/ml, increased the longitudinal tension and the resistance to flow through the urethras. The latter effect was less pronounced than that produced by noradrenaline, especially in the guinea-pig preparations. Noradrenaline was also more effective than ecetylcholine in contracting the circular muscle of the urethra. The effect of acetylcholine was blocked by atropine, 0-1 mug/ml, suggesting that the contractions were mediated through muscarinic cholinoceptors.", "PMID": 975629}
{"id": "pubmed23n0032_10384", "title": "Effect of propranolol on the release of choline from guinea-pig small intestine in vitro.", "content": "1. Choline was released into the fluid bathing the serosal surface of segments of guinea-pig small intestine. The mean rate of release (nmol choline per cm of intestine per h) in the first hour was 10-2 and in the second hour 8-0. 2. Propranolol 1 mM in the mucosal fluid caused a significant (P less than 0-01) increase in the rate at which choline was released into the serosal fluid. The mean rates were 15-4 and 19-2 for the first and second hour, respectively. 3. It is suggested that the effect of propranolol observed in the present study is likely to be due to a non-specific direct action on biological membranes.", "contents": "Effect of propranolol on the release of choline from guinea-pig small intestine in vitro. 1. Choline was released into the fluid bathing the serosal surface of segments of guinea-pig small intestine. The mean rate of release (nmol choline per cm of intestine per h) in the first hour was 10-2 and in the second hour 8-0. 2. Propranolol 1 mM in the mucosal fluid caused a significant (P less than 0-01) increase in the rate at which choline was released into the serosal fluid. The mean rates were 15-4 and 19-2 for the first and second hour, respectively. 3. It is suggested that the effect of propranolol observed in the present study is likely to be due to a non-specific direct action on biological membranes.", "PMID": 975626}
{"id": "pubmed23n0032_10385", "title": "The effects of propranolol, practolol and metoprolol on exercise-induced tachycardia in relation to plasma levels in man.", "content": "1. The effects of single oral doses of propranolol, practolol and a new cardioselective beta-adrenoceptor blocking drug, metoprolol, on exercise-induced tachycardia in relation to plasma levels were studied in six normal volunteers. 2. Exercise undertaken on treadmill was submaximal which, under control conditions, increased the heart rate from 74-3 (s.e.m. = 6-8) to 153-8 (s.e.m. = 9.8) beats/min. 3. Plasma concentrations of propranolol and practolol were assayed fluorometrically and of metoprolol by electron-capture gas liquid chromatography, the details of which are described. 4. Between 1-5 and 2 h after drug ingestion 80 mg of propranolol associated with plasma level of 50-60 ng/ml (half-life 2-75 h), reduced the exercise-induced tachycardia by 27%, 250 mg of practolol with plasma levels of 1050-1100 ng/ml reduced it by 28% and 100 mg of metoprolol with plasma concentrations of 140-150 ng/ml (half-life 1-7 h), reduced it by 30%. 5. The resting heart rates were reduced significantly by propranolol and metoprolol but not by practolol. 6. Metoprolol is a potent short-acting beta-adrenoceptor antagonist; its advantages as a cardioselective agent over practolol in therapeutic use are discussed.", "contents": "The effects of propranolol, practolol and metoprolol on exercise-induced tachycardia in relation to plasma levels in man. 1. The effects of single oral doses of propranolol, practolol and a new cardioselective beta-adrenoceptor blocking drug, metoprolol, on exercise-induced tachycardia in relation to plasma levels were studied in six normal volunteers. 2. Exercise undertaken on treadmill was submaximal which, under control conditions, increased the heart rate from 74-3 (s.e.m. = 6-8) to 153-8 (s.e.m. = 9.8) beats/min. 3. Plasma concentrations of propranolol and practolol were assayed fluorometrically and of metoprolol by electron-capture gas liquid chromatography, the details of which are described. 4. Between 1-5 and 2 h after drug ingestion 80 mg of propranolol associated with plasma level of 50-60 ng/ml (half-life 2-75 h), reduced the exercise-induced tachycardia by 27%, 250 mg of practolol with plasma levels of 1050-1100 ng/ml reduced it by 28% and 100 mg of metoprolol with plasma concentrations of 140-150 ng/ml (half-life 1-7 h), reduced it by 30%. 5. The resting heart rates were reduced significantly by propranolol and metoprolol but not by practolol. 6. Metoprolol is a potent short-acting beta-adrenoceptor antagonist; its advantages as a cardioselective agent over practolol in therapeutic use are discussed.", "PMID": 975632}
{"id": "pubmed23n0032_10386", "title": "The action of histamine on pulmonary vessels of cats and rats.", "content": "1. The actions of histamine on pulmonary vascular smooth muscle have been studied in isolated cat and rat lungs perfused with blood, lobes of cat lung perfused in vivo and isolated strips of rat, cat and rabbit pulmonary artery. 2. In all the lung preparations histamine caused both dilatation and constriction. In the rat strips it caused both contraction and relaxation. Dilatation was only well shown when the vessels were in a prior constricted state. In any one lung dilatation occurred with smaller doses than constriction. 3. Histamine caused both increases and decreases in pulmonary artery pressure in collapsed lungs. In this condition these effects are unlikely to have been a consequence of changes in airway pressure. 4. From forward and reverse perfusions of lungs in the waterfall state, where changes in postalveolar vessels do not affect pulmonary artery pressure, it appeared that histamine caused both dilatation and constriction on both sides of the point of collapse caused by alveolar pressure. 5. Plots of the relationship between left atrial and pulmonary artery pressure (at constant alveolar pressure and blood flow) showed that histamine caused both increases and decreases in pulmonary vascular resistance and sometimes also increased the \"Starling resistor\" properties of lung vessels. 6. In plethysmograph experiments histamine caused moderate dilatation and constriction without affecting lung volume but strong vasoconstriction was accompanied by increases in lung volume.", "contents": "The action of histamine on pulmonary vessels of cats and rats. 1. The actions of histamine on pulmonary vascular smooth muscle have been studied in isolated cat and rat lungs perfused with blood, lobes of cat lung perfused in vivo and isolated strips of rat, cat and rabbit pulmonary artery. 2. In all the lung preparations histamine caused both dilatation and constriction. In the rat strips it caused both contraction and relaxation. Dilatation was only well shown when the vessels were in a prior constricted state. In any one lung dilatation occurred with smaller doses than constriction. 3. Histamine caused both increases and decreases in pulmonary artery pressure in collapsed lungs. In this condition these effects are unlikely to have been a consequence of changes in airway pressure. 4. From forward and reverse perfusions of lungs in the waterfall state, where changes in postalveolar vessels do not affect pulmonary artery pressure, it appeared that histamine caused both dilatation and constriction on both sides of the point of collapse caused by alveolar pressure. 5. Plots of the relationship between left atrial and pulmonary artery pressure (at constant alveolar pressure and blood flow) showed that histamine caused both increases and decreases in pulmonary vascular resistance and sometimes also increased the \"Starling resistor\" properties of lung vessels. 6. In plethysmograph experiments histamine caused moderate dilatation and constriction without affecting lung volume but strong vasoconstriction was accompanied by increases in lung volume.", "PMID": 975628}
{"id": "pubmed23n0032_10387", "title": "Lethal effects of reserpine plus physostigmine and neostigmine in mice.", "content": "1. The interaction between the reversible cholinesterase inhibitors, physostigmine and neostigmine and reserpine was studied. 2. A combination of reserpine plus a cholinesterase inhibitor significantly increased lethality in adult male Swiss-Webster mice above that caused by either neostigmine, physostigmine, or reserpine alone. 3. Methscopolamine completely reversed this effect. 4. It would appear that the presence of the antiadrenergic agent, reserpine, increases the toxicity of cholinomimetic agents. 5. The above results may have clinical significance, since reserpine and cholinesterase inhibitors are used in the practice of medicine.", "contents": "Lethal effects of reserpine plus physostigmine and neostigmine in mice. 1. The interaction between the reversible cholinesterase inhibitors, physostigmine and neostigmine and reserpine was studied. 2. A combination of reserpine plus a cholinesterase inhibitor significantly increased lethality in adult male Swiss-Webster mice above that caused by either neostigmine, physostigmine, or reserpine alone. 3. Methscopolamine completely reversed this effect. 4. It would appear that the presence of the antiadrenergic agent, reserpine, increases the toxicity of cholinomimetic agents. 5. The above results may have clinical significance, since reserpine and cholinesterase inhibitors are used in the practice of medicine.", "PMID": 975633}
{"id": "pubmed23n0032_10388", "title": "Plasma carbamazepine determinations: a simple gas chromatographic method.", "content": "1. A simple, rapid and specific gas chromatographic method for the measurement of plasma carbamazepine concentration is presented. 2. The extraction is a single step method, and the drug is measured by derivatization using on column methylation and standard instrumentation. 3. Other anticonvulsant agents do not co-extract. Other drugs do not interfere in the chromatogram. 4. Stability, recovery and reproducibility make this procedure suitable for routine carbamazepine measurements.", "contents": "Plasma carbamazepine determinations: a simple gas chromatographic method. 1. A simple, rapid and specific gas chromatographic method for the measurement of plasma carbamazepine concentration is presented. 2. The extraction is a single step method, and the drug is measured by derivatization using on column methylation and standard instrumentation. 3. Other anticonvulsant agents do not co-extract. Other drugs do not interfere in the chromatogram. 4. Stability, recovery and reproducibility make this procedure suitable for routine carbamazepine measurements.", "PMID": 975635}
{"id": "pubmed23n0032_10389", "title": "Circulating dopamine-beta-hydroxylase (DbetaH) and catecholamines in a paediatric phaeochromocytoma.", "content": "1. Circulating catecholamines and dopamine-beta-hydroxylase (DbetaH) have been studied in a child with bilateral phaeochromocytoma. 2. Venous catheterization showed a great increase in catecholamine efflux from the left adrenal vein while DbetaH activity in the latter was only slightly elevated. 3. Circulating catecholamines fluctated greatly while DbetaH activity decreased gradually during the removal of the tumours. 4. Post-operatively, circulating catecholamines declined to normal values while DbetaH activity returned to pre-operative levels. 5. It is concluded that these tumours released circulating catecholamines independent of an exocytotic secretion of DbetaH and that the circulating DbetaH reflected the activity of the sympathetic nervous system.", "contents": "Circulating dopamine-beta-hydroxylase (DbetaH) and catecholamines in a paediatric phaeochromocytoma. 1. Circulating catecholamines and dopamine-beta-hydroxylase (DbetaH) have been studied in a child with bilateral phaeochromocytoma. 2. Venous catheterization showed a great increase in catecholamine efflux from the left adrenal vein while DbetaH activity in the latter was only slightly elevated. 3. Circulating catecholamines fluctated greatly while DbetaH activity decreased gradually during the removal of the tumours. 4. Post-operatively, circulating catecholamines declined to normal values while DbetaH activity returned to pre-operative levels. 5. It is concluded that these tumours released circulating catecholamines independent of an exocytotic secretion of DbetaH and that the circulating DbetaH reflected the activity of the sympathetic nervous system.", "PMID": 975634}
{"id": "pubmed23n0032_10390", "title": "Effects of amiodarone and L8040, novel antianginal and antiarrhythmic drugs, on cardiac and coronary haemodynamics and on cardiac intracellular potentials.", "content": "1. The effects on the coronary and systemic haemodynamics of intravenous and intracoronary injections of two benzfuran derivatives, amiodarone and its brominated analogue (L8040), were studied in open-chest anaesthetized dogs. The effects of L8040 on cardiac intracellular potentials after 6 weeks of 20 mg/kg intraperitoneal injections in rabbits were also investigated. 2. Both compounds produced dose-related and quantitatively similar decreases in coronary vascular resistance following their intracoronary administration; threshold effects occurred with about 0-25 mg of each drug and maximal effects with 4 mg. Larger intracoronary doses produced measurable systemic effects. 3. Intravenous injections of amiodarone and L8040 (2-5-10 mg/kg) produced dose-related decreases in heart rate and aortic pressure with a fall in total peripheral resistance. The left ventricular output was either unaffected or increased with a consistent augmentation in stroke volume. 4. The bradycardia produced by both drugs was associated with prolongation of the P-R interval of the electrocardiogram with no significant effect on the QRS duration or the Q-T interval. 5. Each drug produced a decrease in the total peripheral vascular resistance with no change  in left ventricular end diastolic pressure except after 10 mg/kg doses which led to an increase in this parameter. 6. Cardiac contractile force and peak LV dp/dt were reduced by both drugs in a dose-related manner. 7. Chronic intraperitoneal administration of L8040 in rabbits caused a prolongation of the duration of the atrial and ventricular intracellular potential without an effect on the maximal rate of depolarization. 8. The effect of amiodarone or L8040 on the coronary circulation and arterial pressure may be attributed to their vasodilator properties but their depressant actions on cardiac contractile force and peak LV dp/dt with an increase in left ventricular end diastolic pressure at high doses, also suggest intrinsic negative inotropic propensity for both compounds. 9. It is concluded that the overall effects on coronary and systemic haemodynamics of amiodarone and its brominated analogue are likely to permit a favourable influence on the balance of oxygen supply and demand in myocardial ischaemia; in addition, their actions on sino-atrial and atrio-ventricular conduction as well as those on cardiac repolarization suggest potential antiarrhythmic properties which merit investigation.", "contents": "Effects of amiodarone and L8040, novel antianginal and antiarrhythmic drugs, on cardiac and coronary haemodynamics and on cardiac intracellular potentials. 1. The effects on the coronary and systemic haemodynamics of intravenous and intracoronary injections of two benzfuran derivatives, amiodarone and its brominated analogue (L8040), were studied in open-chest anaesthetized dogs. The effects of L8040 on cardiac intracellular potentials after 6 weeks of 20 mg/kg intraperitoneal injections in rabbits were also investigated. 2. Both compounds produced dose-related and quantitatively similar decreases in coronary vascular resistance following their intracoronary administration; threshold effects occurred with about 0-25 mg of each drug and maximal effects with 4 mg. Larger intracoronary doses produced measurable systemic effects. 3. Intravenous injections of amiodarone and L8040 (2-5-10 mg/kg) produced dose-related decreases in heart rate and aortic pressure with a fall in total peripheral resistance. The left ventricular output was either unaffected or increased with a consistent augmentation in stroke volume. 4. The bradycardia produced by both drugs was associated with prolongation of the P-R interval of the electrocardiogram with no significant effect on the QRS duration or the Q-T interval. 5. Each drug produced a decrease in the total peripheral vascular resistance with no change  in left ventricular end diastolic pressure except after 10 mg/kg doses which led to an increase in this parameter. 6. Cardiac contractile force and peak LV dp/dt were reduced by both drugs in a dose-related manner. 7. Chronic intraperitoneal administration of L8040 in rabbits caused a prolongation of the duration of the atrial and ventricular intracellular potential without an effect on the maximal rate of depolarization. 8. The effect of amiodarone or L8040 on the coronary circulation and arterial pressure may be attributed to their vasodilator properties but their depressant actions on cardiac contractile force and peak LV dp/dt with an increase in left ventricular end diastolic pressure at high doses, also suggest intrinsic negative inotropic propensity for both compounds. 9. It is concluded that the overall effects on coronary and systemic haemodynamics of amiodarone and its brominated analogue are likely to permit a favourable influence on the balance of oxygen supply and demand in myocardial ischaemia; in addition, their actions on sino-atrial and atrio-ventricular conduction as well as those on cardiac repolarization suggest potential antiarrhythmic properties which merit investigation.", "PMID": 975630}
{"id": "pubmed23n0032_10391", "title": "The effects of mazindol and 46-034 (Sandoz) on glucose oxidation and insulin binding by rat isolated fat cells.", "content": "1. The anorexic agent mazindol and its major metabolite 46-034 (Sandoz) in high concentrations ( greater than 0-4 mM) abolished basal and insulin-stimulated conversion of 1-14C-glucose to 14CO2 by rat isolated fat cells. 2. High concentrations (1mM) also inhibited specific binding of 125 I-insulin to fat cells. 3. The observed effects appeared to be due in part to perturbation of the plasma membrane since there was a rise in the lactate dehydrogenase content of the incubation medium, increased 125I-insulin degradation and a reduction in cellular tritiated water space. 4. These effects are unlikely to be relevant to the therapeutic action of mazindol.", "contents": "The effects of mazindol and 46-034 (Sandoz) on glucose oxidation and insulin binding by rat isolated fat cells. 1. The anorexic agent mazindol and its major metabolite 46-034 (Sandoz) in high concentrations ( greater than 0-4 mM) abolished basal and insulin-stimulated conversion of 1-14C-glucose to 14CO2 by rat isolated fat cells. 2. High concentrations (1mM) also inhibited specific binding of 125 I-insulin to fat cells. 3. The observed effects appeared to be due in part to perturbation of the plasma membrane since there was a rise in the lactate dehydrogenase content of the incubation medium, increased 125I-insulin degradation and a reduction in cellular tritiated water space. 4. These effects are unlikely to be relevant to the therapeutic action of mazindol.", "PMID": 975636}
{"id": "pubmed23n0032_10392", "title": "Changes of bone histology during maintenance hemodialysis at various levels of dialyzate Ca concentration.", "content": "Bone histology (iliac crest biopsies; undecalcified sections; micromorphometry) was studied by repeated biopsies in 19 patients on maintenance hemodialysis. Sampling error was assessed by taking two biopsies at one time. After an initial biopsy, patients were dialyzed either against 3.6 mEq Ca/l dialyzate or 3.9 mEq Ca/l dialyzate for 9-16 months before a second biopsy was taken. Bone histology failed to normalize irrespective of the dialyzate Ca concentration. Surface density of endosteal fibrosis diminished in a considerable proportion of the patients (9/19). Volumetric density of osteoid fell in 5 and rose in 4 of the patients.", "contents": "Changes of bone histology during maintenance hemodialysis at various levels of dialyzate Ca concentration. Bone histology (iliac crest biopsies; undecalcified sections; micromorphometry) was studied by repeated biopsies in 19 patients on maintenance hemodialysis. Sampling error was assessed by taking two biopsies at one time. After an initial biopsy, patients were dialyzed either against 3.6 mEq Ca/l dialyzate or 3.9 mEq Ca/l dialyzate for 9-16 months before a second biopsy was taken. Bone histology failed to normalize irrespective of the dialyzate Ca concentration. Surface density of endosteal fibrosis diminished in a considerable proportion of the patients (9/19). Volumetric density of osteoid fell in 5 and rose in 4 of the patients.", "PMID": 975642}
{"id": "pubmed23n0032_10393", "title": "Circulatory changes in cold-acclimation and cold stress.", "content": "1. The cardiovascular changes of conscious cold-acclimated (CA) and warm-acclimated (WA) rats during exposure to 5 degrees C or 28 degrees C were studied. 2. The cardiac output, heart rate and stroke volume of Ca rats exposed to 5 degrees C and of WA rats during cold stress were significantly greater, and their calculated total peripheral resistance significantly less than those of WA rats ats exposed to 28 degrees C. These results show that circulatory changes participate in cold acclimation and cold stress. The circulatory changes in the two conditions were compared and the mechanism of the observed differences were discussed. 3. CA rats exposed to 28 degrees C showed a striking decrease of oxygen consumption and arterio-venous O2 difference, but significant circulatory changes were decreased heart rate and cardiac index only, indicating that the response was mainly metabolic.", "contents": "Circulatory changes in cold-acclimation and cold stress. 1. The cardiovascular changes of conscious cold-acclimated (CA) and warm-acclimated (WA) rats during exposure to 5 degrees C or 28 degrees C were studied. 2. The cardiac output, heart rate and stroke volume of Ca rats exposed to 5 degrees C and of WA rats during cold stress were significantly greater, and their calculated total peripheral resistance significantly less than those of WA rats ats exposed to 28 degrees C. These results show that circulatory changes participate in cold acclimation and cold stress. The circulatory changes in the two conditions were compared and the mechanism of the observed differences were discussed. 3. CA rats exposed to 28 degrees C showed a striking decrease of oxygen consumption and arterio-venous O2 difference, but significant circulatory changes were decreased heart rate and cardiac index only, indicating that the response was mainly metabolic.", "PMID": 975631}
{"id": "pubmed23n0032_10394", "title": "The binding of chloramphenicol to serum proteins in patients with chronic renal insufficiency.", "content": "The binding (r) of chloramphenicol to serum proteins is significantly lower in patients with chronic renal failure than in normal subjects. Before hemodialysis, the mean r value in patients with chronic renal insufficiency was 0.165 mg/g (+/-0.003) versus 0.188 mg/g (+/-0.004) in healthy individuals. Hemodialysis produced a significant rise in r (to 0.182 mg/g, +/-0.004). Decrease in the serum concentration of albumin in patients with chronic renal insufficiency does not seem to be the sole factor responsible for decreased r.", "contents": "The binding of chloramphenicol to serum proteins in patients with chronic renal insufficiency. The binding (r) of chloramphenicol to serum proteins is significantly lower in patients with chronic renal failure than in normal subjects. Before hemodialysis, the mean r value in patients with chronic renal insufficiency was 0.165 mg/g (+/-0.003) versus 0.188 mg/g (+/-0.004) in healthy individuals. Hemodialysis produced a significant rise in r (to 0.182 mg/g, +/-0.004). Decrease in the serum concentration of albumin in patients with chronic renal insufficiency does not seem to be the sole factor responsible for decreased r.", "PMID": 975643}
{"id": "pubmed23n0032_10395", "title": "Quadricepsplasty: a review, case presentations, and discussion.", "content": "The operation of quadricepsplasty as described by T. C. Thompson, if followed by satisfactory after-treatment, can be expected to lead to a substantial gain of knee motion in patients who have fibrous ankylosis of the knee.", "contents": "Quadricepsplasty: a review, case presentations, and discussion. The operation of quadricepsplasty as described by T. C. Thompson, if followed by satisfactory after-treatment, can be expected to lead to a substantial gain of knee motion in patients who have fibrous ankylosis of the knee.", "PMID": 975644}
{"id": "pubmed23n0032_10396", "title": "Complications of polycentric knee arthroplasty.", "content": "Results of 89 polycentric knee arthroplasties are assessed from 2 years to 7 1/2 years after operation. Seventy-two arthroplasties (81%) are considered to improve the mobility of the patient, with 17 arthroplasties producing no improvement. Infection after operation in 6 arthroplasties (7%) required arthrodesis of 3 arthroplasties. Loosening of prosthetic components occurred in 9 arthroplasties (10%).", "contents": "Complications of polycentric knee arthroplasty. Results of 89 polycentric knee arthroplasties are assessed from 2 years to 7 1/2 years after operation. Seventy-two arthroplasties (81%) are considered to improve the mobility of the patient, with 17 arthroplasties producing no improvement. Infection after operation in 6 arthroplasties (7%) required arthrodesis of 3 arthroplasties. Loosening of prosthetic components occurred in 9 arthroplasties (10%).", "PMID": 975645}
{"id": "pubmed23n0032_10397", "title": "Diphosphonate treatment of Paget's disease.", "content": "Ethane-1-hydroxy-1, 1-diphosphonate (EHDP) was administered in a dose of 20 mg/kg/d to 21 patients with symptomatic Paget's disease. All patients were treated for 6 months and then followed for an additional 6 months. There was a striking decline in serum alkaline phosphatase and urinary hydroxy-proline excretion observed after 3 months of therapy which was not significantly improved in the succeeding 3 months. Concomitantly there was marked improvement in clinical symptoms and bone scans. Following cessation of therapy, continued biochemical and clinical evidence of remission persisted. Several patients on repeat treatment with EHDP appeared to respond promptly. Side effects were minimal except for a possibly related osteomalacia and increased incidence of pathologic fractures.", "contents": "Diphosphonate treatment of Paget's disease. Ethane-1-hydroxy-1, 1-diphosphonate (EHDP) was administered in a dose of 20 mg/kg/d to 21 patients with symptomatic Paget's disease. All patients were treated for 6 months and then followed for an additional 6 months. There was a striking decline in serum alkaline phosphatase and urinary hydroxy-proline excretion observed after 3 months of therapy which was not significantly improved in the succeeding 3 months. Concomitantly there was marked improvement in clinical symptoms and bone scans. Following cessation of therapy, continued biochemical and clinical evidence of remission persisted. Several patients on repeat treatment with EHDP appeared to respond promptly. Side effects were minimal except for a possibly related osteomalacia and increased incidence of pathologic fractures.", "PMID": 975646}
{"id": "pubmed23n0032_10398", "title": "Curettage and acrylic cementation in surgery of giant cell tumors of bone.", "content": "True giant cell tumors of bone (osteoblastomas) are comparatively rare but often large at time of discovery, making the surgical treatment controversial. Simple curettage or resection with bone filling is used when the lesion is small. Resection with arthroplasty or arthrodesis and possibly amputation is used in advanced cases. Thorough curettage with acrylic cementation is presented in a series of 6 cases from Rotterdam and Lund with a follow-up time between 2 and 7 years. The advantages of this method are full and early mobility and stability as well as a simplified postoperative X-ray control, especially when barium sulphate contrast has been added to the acrylic cement. Finally, if this technique is used in cases where more extensive surgery becomes necessary later, there is much to gain and little to lose. The operation may turn out to be the method of choice for this special type of tumor when a simple curettage or resection is no longer possible without loss of function. No recurrences or spread have been observed among these 6 cases. It is suggested that the heat generation could be an advantage of the method.", "contents": "Curettage and acrylic cementation in surgery of giant cell tumors of bone. True giant cell tumors of bone (osteoblastomas) are comparatively rare but often large at time of discovery, making the surgical treatment controversial. Simple curettage or resection with bone filling is used when the lesion is small. Resection with arthroplasty or arthrodesis and possibly amputation is used in advanced cases. Thorough curettage with acrylic cementation is presented in a series of 6 cases from Rotterdam and Lund with a follow-up time between 2 and 7 years. The advantages of this method are full and early mobility and stability as well as a simplified postoperative X-ray control, especially when barium sulphate contrast has been added to the acrylic cement. Finally, if this technique is used in cases where more extensive surgery becomes necessary later, there is much to gain and little to lose. The operation may turn out to be the method of choice for this special type of tumor when a simple curettage or resection is no longer possible without loss of function. No recurrences or spread have been observed among these 6 cases. It is suggested that the heat generation could be an advantage of the method.", "PMID": 975647}
{"id": "pubmed23n0032_10399", "title": "Spontaneous fractures in the brain-crippled, bedridden patient.", "content": "This report deals with the treatment of 31 spontaneous fractures which occurred in 50 institutionalized patients who were bedridden primarily because of severe cerebral palsy associated with brain injury. More than one-half of the group sustained a spontaneous fracture. Satisfactory healing of all fractures with a minimum of complications occurred without any immobilization or realignment. In 4 patients with delayed compound wounds, the treatment consisted of resection of the protruding portion of the bone after allowing the bony protrusion to wall itself off.", "contents": "Spontaneous fractures in the brain-crippled, bedridden patient. This report deals with the treatment of 31 spontaneous fractures which occurred in 50 institutionalized patients who were bedridden primarily because of severe cerebral palsy associated with brain injury. More than one-half of the group sustained a spontaneous fracture. Satisfactory healing of all fractures with a minimum of complications occurred without any immobilization or realignment. In 4 patients with delayed compound wounds, the treatment consisted of resection of the protruding portion of the bone after allowing the bony protrusion to wall itself off.", "PMID": 975648}
{"id": "pubmed23n0032_10400", "title": "Valgus osteotomy for osteoarthritis of the knee.", "content": "Recent reports indicate that proximal tibial osteotomy for osteoarthritis of the knee is contraindicated if subluxation of the joint is evident clinically or on weight-bearing X-rays, or if the angular deformity of the joint is greater than 15 degrees. The purpose of this paper is to present evidence that a proximal tibial osteotomy, properly planned and performed, can give consistently sat-sfactory results even in osteoarthritic knees which have preoperative varus deformities greater that 15 degrees or are subluxated. A proper planning requires an exact measurement of the deformity on full length X-rays, a precise preoperative drawing and a very exact osteotomy giving some overcorrection. The overcorrection must compensate for the weakness of the lateral muscles, the most frequent cause of the condition.", "contents": "Valgus osteotomy for osteoarthritis of the knee. Recent reports indicate that proximal tibial osteotomy for osteoarthritis of the knee is contraindicated if subluxation of the joint is evident clinically or on weight-bearing X-rays, or if the angular deformity of the joint is greater than 15 degrees. The purpose of this paper is to present evidence that a proximal tibial osteotomy, properly planned and performed, can give consistently sat-sfactory results even in osteoarthritic knees which have preoperative varus deformities greater that 15 degrees or are subluxated. A proper planning requires an exact measurement of the deformity on full length X-rays, a precise preoperative drawing and a very exact osteotomy giving some overcorrection. The overcorrection must compensate for the weakness of the lateral muscles, the most frequent cause of the condition.", "PMID": 975649}
{"id": "pubmed23n0032_10401", "title": "Total condylar knee replacment: preliminary report.", "content": "The Total Condylar Prosthesis is a non-hinged unit designed to: replace the patellofemoral articulation; improve fixation of the tibial component by means of a stout central peg; permit accurate and reproducible insertion. The cruciate ligaments are excised to obtain better tibial fixation. Instability has not been a problem except in a few cases of a technical error (removal of excessive bone from the tibia creates instability in flexion). The prosthesis cannot be positioned by \"eye\" and the instrumentation must be applied exactly.", "contents": "Total condylar knee replacment: preliminary report. The Total Condylar Prosthesis is a non-hinged unit designed to: replace the patellofemoral articulation; improve fixation of the tibial component by means of a stout central peg; permit accurate and reproducible insertion. The cruciate ligaments are excised to obtain better tibial fixation. Instability has not been a problem except in a few cases of a technical error (removal of excessive bone from the tibia creates instability in flexion). The prosthesis cannot be positioned by \"eye\" and the instrumentation must be applied exactly.", "PMID": 975650}
{"id": "pubmed23n0032_10402", "title": "Displaced fracture of the lumbosacral spine with delayed cauda equina deficit: report of a case and review of literature.", "content": "A 41-year-old man with a fracture of the upper sacrum and forward and downward displacement of the superior sacral fragment and upper spine on the lower sacrum, developed partial deficit of cauda equina function. Similar fractures rarely have been reported. Signs of lumbar pain, contusion, and fractured transverse processes should lead the surgeon to carefully examine the radiologic fractures of the area of injury; otherwise, this lesion may heal unrecognized. With the spine in flexion, the fracture may create mechanical instability. Early closed or open reduction is theoretically ideal. Surgical treatment must be weighed in terms of the risks and needs of the individual.", "contents": "Displaced fracture of the lumbosacral spine with delayed cauda equina deficit: report of a case and review of literature. A 41-year-old man with a fracture of the upper sacrum and forward and downward displacement of the superior sacral fragment and upper spine on the lower sacrum, developed partial deficit of cauda equina function. Similar fractures rarely have been reported. Signs of lumbar pain, contusion, and fractured transverse processes should lead the surgeon to carefully examine the radiologic fractures of the area of injury; otherwise, this lesion may heal unrecognized. With the spine in flexion, the fracture may create mechanical instability. Early closed or open reduction is theoretically ideal. Surgical treatment must be weighed in terms of the risks and needs of the individual.", "PMID": 975651}
{"id": "pubmed23n0032_10403", "title": "Tibial torsion?", "content": "A longitudinal study evaluating the degree of rotation of the bimalleolar axis of tibia and fibula at birth, 6 month, 12 months and 24 months revealed a gradual statistically significant external rotation from a mean of 4 degrees at birth to a mean of 11 degrees at 24 months. There was no correlation with the sex or race of the child or with the age at which the child began independent walking. It is concluded that this external rotation is a part of normal growth and development of the infants' lower extremities.", "contents": "Tibial torsion? A longitudinal study evaluating the degree of rotation of the bimalleolar axis of tibia and fibula at birth, 6 month, 12 months and 24 months revealed a gradual statistically significant external rotation from a mean of 4 degrees at birth to a mean of 11 degrees at 24 months. There was no correlation with the sex or race of the child or with the age at which the child began independent walking. It is concluded that this external rotation is a part of normal growth and development of the infants' lower extremities.", "PMID": 975652}
{"id": "pubmed23n0032_10404", "title": "Vertical transarticular pin fixation for unstable ankle fractures: impressions after 16 years of experience.", "content": "The usage of vertical transarticular pin fixation combined with plaster immobilization for severe ankle injuries has been reviewed in 92 consecutive cases over a period of 16 years. Moderately simple in application and without a skin incision, the procedure has been found to provide efficient and reliable short-term stabilization of the ankle and subtalar joints with minimal complications. A new technique is described for accurate placement of the pin and the prevention of its migration. It is a reasonable alternative treatment for certain unstable distal tibial and ankle fractures when open reduction with conventional internal fixation cannot be done. In particular, the method is recommended in treating displaced fractures at the ankle and deltoid ligament ruptures in geriatric patients. It is often useful as a salvage or last-resort procedure in unusually severe ankle injuries with circulatory or neural deficits. In many instances additional trauma to the head, thorax or abdomen may have been sustained. Thus total patient care is essential and priorities must be recognized. The treatment of ankle trauma, furthermore, in a physiologically-aged diabetic patient is often not the same as that for similar injuries in a young healthy adult. The clinical judgement and experience of the operator is the basis for selection of the procedure. The simplest method is often the best. Pin fixation, however, is not for everyone. It should be done only by surgeons qualified to treat bone and joint trauma and even then only for those situations in which its usage is specifically indicated.", "contents": "Vertical transarticular pin fixation for unstable ankle fractures: impressions after 16 years of experience. The usage of vertical transarticular pin fixation combined with plaster immobilization for severe ankle injuries has been reviewed in 92 consecutive cases over a period of 16 years. Moderately simple in application and without a skin incision, the procedure has been found to provide efficient and reliable short-term stabilization of the ankle and subtalar joints with minimal complications. A new technique is described for accurate placement of the pin and the prevention of its migration. It is a reasonable alternative treatment for certain unstable distal tibial and ankle fractures when open reduction with conventional internal fixation cannot be done. In particular, the method is recommended in treating displaced fractures at the ankle and deltoid ligament ruptures in geriatric patients. It is often useful as a salvage or last-resort procedure in unusually severe ankle injuries with circulatory or neural deficits. In many instances additional trauma to the head, thorax or abdomen may have been sustained. Thus total patient care is essential and priorities must be recognized. The treatment of ankle trauma, furthermore, in a physiologically-aged diabetic patient is often not the same as that for similar injuries in a young healthy adult. The clinical judgement and experience of the operator is the basis for selection of the procedure. The simplest method is often the best. Pin fixation, however, is not for everyone. It should be done only by surgeons qualified to treat bone and joint trauma and even then only for those situations in which its usage is specifically indicated.", "PMID": 975653}
{"id": "pubmed23n0032_10405", "title": "Forearm fractures in children: pitfalls and complications.", "content": "Five hundred fourty-seven consecutive forearm fractures in children were studied, with special emphasis on complications encountered. The most important apparent conclusion reached from this study is that greenstick and complete fractures are different, and that some of the pitfalls and complications seen following these injuries can be avoided if different methods of reduction are used for each. Greenstick fractures should be reduced by manipulating them into the correct proper plane of rotation; i.e., maximum pronation for distal third fractures, neutral for middle third fractures, and supination for proximal third fractures. Application of this familiar \"rule of thirds\" to complete fractures, however, may result in ratational deformity at the fracture site. Completer fractures should be reduced by finger trap traction, allowing the fracture to seek its own level of rotation. Several other observations were also noted. Angulation into radial deviation was found to remodel and equally as well as volar angulation, and remodeling may contine for as long as two years after injury. Growth disturbance following fractures through the distal radial epiphyseal plate is uncommon, but premature closure of the epiphysis did occur in one patient with a typical Type II fracture. Six concomitant nerve injuries were seen; all recovered spontaneously within 3 weeks.", "contents": "Forearm fractures in children: pitfalls and complications. Five hundred fourty-seven consecutive forearm fractures in children were studied, with special emphasis on complications encountered. The most important apparent conclusion reached from this study is that greenstick and complete fractures are different, and that some of the pitfalls and complications seen following these injuries can be avoided if different methods of reduction are used for each. Greenstick fractures should be reduced by manipulating them into the correct proper plane of rotation; i.e., maximum pronation for distal third fractures, neutral for middle third fractures, and supination for proximal third fractures. Application of this familiar \"rule of thirds\" to complete fractures, however, may result in ratational deformity at the fracture site. Completer fractures should be reduced by finger trap traction, allowing the fracture to seek its own level of rotation. Several other observations were also noted. Angulation into radial deviation was found to remodel and equally as well as volar angulation, and remodeling may contine for as long as two years after injury. Growth disturbance following fractures through the distal radial epiphyseal plate is uncommon, but premature closure of the epiphysis did occur in one patient with a typical Type II fracture. Six concomitant nerve injuries were seen; all recovered spontaneously within 3 weeks.", "PMID": 975654}
{"id": "pubmed23n0032_10406", "title": "A statistical evaluation of polycentric total knee arthroplasties.", "content": "Four hundred nineteen knees in 299 patients were examined preoperatively and at two years after polycentric total knee surgery. One hundred forty-three knees were in 109 patients with osteoarthritis. Two hundred seventy-six knees were in 190 patients with rheumatoid arthritis. Pain decreased significantly, use of aids decreased significantly, and distance walked increased significantly in both groups. Rheumatoid patients did not fare as well as osteoarthritics in the use of aids and distance walked. Osteoarthritic knees had proportionately more implant settling, implant loosening and reoperations than did rheumatoid arthritic knees.", "contents": "A statistical evaluation of polycentric total knee arthroplasties. Four hundred nineteen knees in 299 patients were examined preoperatively and at two years after polycentric total knee surgery. One hundred forty-three knees were in 109 patients with osteoarthritis. Two hundred seventy-six knees were in 190 patients with rheumatoid arthritis. Pain decreased significantly, use of aids decreased significantly, and distance walked increased significantly in both groups. Rheumatoid patients did not fare as well as osteoarthritics in the use of aids and distance walked. Osteoarthritic knees had proportionately more implant settling, implant loosening and reoperations than did rheumatoid arthritic knees.", "PMID": 975655}
{"id": "pubmed23n0032_10407", "title": "Acute carpal tunnel syndrome secondary to pseudogout: case report.", "content": "Acute carpal tunnel syndrome secondary to chondrocalcinosis seems not to have been previously reported. In a 75-year-old woman, with arthropathy and calcification of the triangular fibrocartilage of the wrist, hyperparathyroidism was suspected, but not proven. Hydroxyapatite and calcium pyrophosphate crystals were found together in the pathological specimen. The patient obtained complete relief from sectioning of the transverse carpal ligament.", "contents": "Acute carpal tunnel syndrome secondary to pseudogout: case report. Acute carpal tunnel syndrome secondary to chondrocalcinosis seems not to have been previously reported. In a 75-year-old woman, with arthropathy and calcification of the triangular fibrocartilage of the wrist, hyperparathyroidism was suspected, but not proven. Hydroxyapatite and calcium pyrophosphate crystals were found together in the pathological specimen. The patient obtained complete relief from sectioning of the transverse carpal ligament.", "PMID": 975656}
{"id": "pubmed23n0032_10408", "title": "Treatment of the triphalangeal thumb.", "content": "The triphalangeal thumb is a rare congenital malformation, but not as rare as the literature would imply. Forty-two patients with 68 triphalangeal thumbs, represent an incidence of about 3 per cent of a population of upper extremity abnormalities. Although little is written about the details of treatment, surgery is frequently necessary. Age makes a great difference as to hom the extra phalanx should be treated. The associated abnormalities present major surgical problems that must be treated early.", "contents": "Treatment of the triphalangeal thumb. The triphalangeal thumb is a rare congenital malformation, but not as rare as the literature would imply. Forty-two patients with 68 triphalangeal thumbs, represent an incidence of about 3 per cent of a population of upper extremity abnormalities. Although little is written about the details of treatment, surgery is frequently necessary. Age makes a great difference as to hom the extra phalanx should be treated. The associated abnormalities present major surgical problems that must be treated early.", "PMID": 975657}
{"id": "pubmed23n0032_10409", "title": "The osteoclast: review of ultrastructure, origin, and structure-function relationship.", "content": "Multinucleated cells of the giant cell tumor and the genuine osteoclasts exhibit a number of common morphological characteristics and may or may not originate from similar progenitor cells. Judging from the present preliminary results, it is obvious that the differentiation of the plasma membrane into a specialized area, the ruffled border, is not as conspicuous in the giant cell as in the osteoclast. A series of interrelated investigations including histochemical methods at the ultrastructural level applied both in giant cell tissue and cultured giant tumor cells are in progress. These studies may further elucidate the possible relationship between the osteoclast and the multinucleated giant cell tumor as well as the possible relationships between giant cells and other cell types of tumors.", "contents": "The osteoclast: review of ultrastructure, origin, and structure-function relationship. Multinucleated cells of the giant cell tumor and the genuine osteoclasts exhibit a number of common morphological characteristics and may or may not originate from similar progenitor cells. Judging from the present preliminary results, it is obvious that the differentiation of the plasma membrane into a specialized area, the ruffled border, is not as conspicuous in the giant cell as in the osteoclast. A series of interrelated investigations including histochemical methods at the ultrastructural level applied both in giant cell tissue and cultured giant tumor cells are in progress. These studies may further elucidate the possible relationship between the osteoclast and the multinucleated giant cell tumor as well as the possible relationships between giant cells and other cell types of tumors.", "PMID": 975659}
{"id": "pubmed23n0032_10410", "title": "Newer knowledge of the immunology of bone and cartilage.", "content": "By the use of modern techniques, the nature of the immunological response to bone and cartilage grafts is becoming clear. Fresh bone, whether cancellous or cortical, will elicit a cell-mediated immunological response; removal of the bone marrow has little effect in reducing immunogenicity. Antibodies against cellular components of the graft are detectable in the recipient only when host and donor have a disparity for the major histocompatibility (H) antigen. Treatment of bone grafts, for the bone bank, by freezing removes their immunogenicity with regard to antibody production but leaves them capable of stimulating the cellmediated immune response. Freeze drying, on the other hand, impairs immunogenicity for both types of responses. Cartilage, grafted alone, is probably non-antigenic as far as both immune responses are concerned and, although there have been a few reports of stimulation of CMI and antibody production by cartilage, these have not been confirmed. Cartilage cells do, however, possess antigens of the major H-antigen system. The cartilage graft is therefore antigenic but only feebly immunogenic, as the matrix proteoglycans protect the cells from the afferent arm of the immune response. Osteoarticular allografts, consisting of both bone and cartilage, sensitize the host due to their bone components. The effect of the immune response upon the bone allograft is to destroy the graft-derived first phase of osteogenesis which, in turn, leads to a poor or non-existent host phase of new bone formation in most allografts. The exact effector mechanism by means of which this destruction is brought about is not known. Bone grafts may be protected from the immune response by use of immunosuppressive measures. Cartilage enjoys a considerable measure of protection from immunological effectors by virtue of its matrix. If this breaks down then the cartilage can become permeable to antibodies. It is suggested that \"lymphokines,\" produced by sensitized lymphocytes, may play some role in destroying the cartilage graft.", "contents": "Newer knowledge of the immunology of bone and cartilage. By the use of modern techniques, the nature of the immunological response to bone and cartilage grafts is becoming clear. Fresh bone, whether cancellous or cortical, will elicit a cell-mediated immunological response; removal of the bone marrow has little effect in reducing immunogenicity. Antibodies against cellular components of the graft are detectable in the recipient only when host and donor have a disparity for the major histocompatibility (H) antigen. Treatment of bone grafts, for the bone bank, by freezing removes their immunogenicity with regard to antibody production but leaves them capable of stimulating the cellmediated immune response. Freeze drying, on the other hand, impairs immunogenicity for both types of responses. Cartilage, grafted alone, is probably non-antigenic as far as both immune responses are concerned and, although there have been a few reports of stimulation of CMI and antibody production by cartilage, these have not been confirmed. Cartilage cells do, however, possess antigens of the major H-antigen system. The cartilage graft is therefore antigenic but only feebly immunogenic, as the matrix proteoglycans protect the cells from the afferent arm of the immune response. Osteoarticular allografts, consisting of both bone and cartilage, sensitize the host due to their bone components. The effect of the immune response upon the bone allograft is to destroy the graft-derived first phase of osteogenesis which, in turn, leads to a poor or non-existent host phase of new bone formation in most allografts. The exact effector mechanism by means of which this destruction is brought about is not known. Bone grafts may be protected from the immune response by use of immunosuppressive measures. Cartilage enjoys a considerable measure of protection from immunological effectors by virtue of its matrix. If this breaks down then the cartilage can become permeable to antibodies. It is suggested that \"lymphokines,\" produced by sensitized lymphocytes, may play some role in destroying the cartilage graft.", "PMID": 975660}
{"id": "pubmed23n0032_10411", "title": "Cell proliferation in the bone marrow and thymus following fractures in rats.", "content": "Skeletal trauma in rats (tibial and femur fractures) stimulates bone marrow and thymus to increased mitotic activity during the first 2-3 days after the fracture. The stimulation is roughly of the same degree as that described in earlier investigations on the effects of calcium ion injections and moderate or major hemorrhages. The cause of increased mitotic level might be due to the release of kinins from the fracture area, factors which are known to stimulate mitoses of the bone marrow and thymus.", "contents": "Cell proliferation in the bone marrow and thymus following fractures in rats. Skeletal trauma in rats (tibial and femur fractures) stimulates bone marrow and thymus to increased mitotic activity during the first 2-3 days after the fracture. The stimulation is roughly of the same degree as that described in earlier investigations on the effects of calcium ion injections and moderate or major hemorrhages. The cause of increased mitotic level might be due to the release of kinins from the fracture area, factors which are known to stimulate mitoses of the bone marrow and thymus.", "PMID": 975661}
{"id": "pubmed23n0032_10412", "title": "Pathogenesis of scoliosis.", "content": "Scoliosis often occurs in otherwise normal individuals or it may be associated with many widely differing diseases. The curve patterns are fairly uniform and the vertebrae always rotate in the frontal and horizontal planes producing convex side rotation with little displacement of the spinuous processes. Many small curves do not increase. Progressive scoliosis increases linearly and the rate of increase accelerates at puberty. No endocrine abnormalities have been observed in these patients. Usually the deformity is not caused by abnormal vertebral growth nor by abnormal collagen in verterbral ligaments. The glycosaminoglycans of nucleus pulposus are decreased in patients with idiopathic scoliosis. We speculate that loss of proteoglycans will affect the viscoelastic properties of the intervertebral discs which may result in permanent deformation. The etiology of scoliosis appears to be multifactorial with a genetic tendency to the deformity which is triggered in different individuals by different factors, some medical, some mechanical and some genetic.", "contents": "Pathogenesis of scoliosis. Scoliosis often occurs in otherwise normal individuals or it may be associated with many widely differing diseases. The curve patterns are fairly uniform and the vertebrae always rotate in the frontal and horizontal planes producing convex side rotation with little displacement of the spinuous processes. Many small curves do not increase. Progressive scoliosis increases linearly and the rate of increase accelerates at puberty. No endocrine abnormalities have been observed in these patients. Usually the deformity is not caused by abnormal vertebral growth nor by abnormal collagen in verterbral ligaments. The glycosaminoglycans of nucleus pulposus are decreased in patients with idiopathic scoliosis. We speculate that loss of proteoglycans will affect the viscoelastic properties of the intervertebral discs which may result in permanent deformation. The etiology of scoliosis appears to be multifactorial with a genetic tendency to the deformity which is triggered in different individuals by different factors, some medical, some mechanical and some genetic.", "PMID": 975662}
{"id": "pubmed23n0032_10413", "title": "A statistical evaluation of geometric total knee arthroplasties.", "content": "Seventy-five knees in 56 patients were examined preoperatively and at two years after geometric total knee surgery. Thirty-three knees were in 28 patients with osteoarthritis. Fourty-two knees were in 28 patients with rheumatoid arthritis. Pain was significantly decreased and the use of aids was significantly decreased in both groups. The distance walked increased significantly for the osteoarthritic group but not for the rheumatoid group. Rheumatoid patients did not fare as well as osteoarthritic patients in distance walked and use of aids. Knees with osteoarthritis had more evidence of lucent lines and implant loosening than did the rheumatoid patients.", "contents": "A statistical evaluation of geometric total knee arthroplasties. Seventy-five knees in 56 patients were examined preoperatively and at two years after geometric total knee surgery. Thirty-three knees were in 28 patients with osteoarthritis. Fourty-two knees were in 28 patients with rheumatoid arthritis. Pain was significantly decreased and the use of aids was significantly decreased in both groups. The distance walked increased significantly for the osteoarthritic group but not for the rheumatoid group. Rheumatoid patients did not fare as well as osteoarthritic patients in distance walked and use of aids. Knees with osteoarthritis had more evidence of lucent lines and implant loosening than did the rheumatoid patients.", "PMID": 975663}
{"id": "pubmed23n0032_10414", "title": "UCI knee replacement.", "content": "Between March 9, 1972 and December 31, 1973, a total of 103 UCI knee replacements were performed. Follow-up data are available on 83 knees with an average follow-up of 33 months. Patient evaluation of the end results indicates that 78.3 per cent were better, 9.6 per cent unchanged, and 12.1 per cent worse. Patient evaluation of their own knee function averaged 55 per cent preoperatively and 79 per cent postoperatively. Patients were also evaluated on a 100 point Modified Larson Analysis Form. The average preoperative score was 46, and the average postoperative score was 70. There were six (5.8%) biological complications in the 103 knee replacement. Biological complications included infections, wound healing problems and unexplained pain. Mechanical complications were seen in 18 (17.4%) knees, and included knee instability, tibial component loosening or deformation, and patellar problems. Additional surgery was required in 18 (17.4%) knees. Failure of the procedure eventually requiring removal of the prosthesis and fusion or amputation occurred in 4 (3.9%) knees. The intermediate-term results of UCI knee replacement have been clinically satisfactory. We currently recommend consideration of this procedure for patients with disabling arthritis of the knee.", "contents": "UCI knee replacement. Between March 9, 1972 and December 31, 1973, a total of 103 UCI knee replacements were performed. Follow-up data are available on 83 knees with an average follow-up of 33 months. Patient evaluation of the end results indicates that 78.3 per cent were better, 9.6 per cent unchanged, and 12.1 per cent worse. Patient evaluation of their own knee function averaged 55 per cent preoperatively and 79 per cent postoperatively. Patients were also evaluated on a 100 point Modified Larson Analysis Form. The average preoperative score was 46, and the average postoperative score was 70. There were six (5.8%) biological complications in the 103 knee replacement. Biological complications included infections, wound healing problems and unexplained pain. Mechanical complications were seen in 18 (17.4%) knees, and included knee instability, tibial component loosening or deformation, and patellar problems. Additional surgery was required in 18 (17.4%) knees. Failure of the procedure eventually requiring removal of the prosthesis and fusion or amputation occurred in 4 (3.9%) knees. The intermediate-term results of UCI knee replacement have been clinically satisfactory. We currently recommend consideration of this procedure for patients with disabling arthritis of the knee.", "PMID": 975664}
{"id": "pubmed23n0032_10415", "title": "Results of knee replacement with the Walldius prosthesis: an interim report.", "content": "Fourty-two knees replaced with the Walldius prosthesis have been followed for an average of almost 6 years. The rating of the average knee on a 100 point clinical scale improved 29 points as a result of the procedure. Complications were carefully documented and were numerous, although relatively few affected the end result.", "contents": "Results of knee replacement with the Walldius prosthesis: an interim report. Fourty-two knees replaced with the Walldius prosthesis have been followed for an average of almost 6 years. The rating of the average knee on a 100 point clinical scale improved 29 points as a result of the procedure. Complications were carefully documented and were numerous, although relatively few affected the end result.", "PMID": 975665}
{"id": "pubmed23n0032_10416", "title": "Guepar hinge prosthesis: complications and results with two years' follow-up.", "content": "Experience with the GUEPAR prosthesis in 292 cases of which 103 have been followed for more than 2 years, suggests that: implanting a hinge prosthesis is major surgery on elderly patients in whom severe complications have occurred and for this reason, the operations should be reserved for extremely damaged and unstable knees; the most important local complications have been deep sepsis for which we have noted a rate of 6.6 per cent; in the treatment of sepsis, everything must be done to preserve the prosthesis because arthrodesis is difficult to obtain; pain relief has been significant as a result of the operation. The prosthetic design allows flexion of more than 90 degrees in 85 per cent of the cases and 120 degrees in 26 per cent; after two years, the results seem relatively stable. We have not observed aseptic loosening after this period but a longer observation period is necessary to be reassured on this point; patellar pain remains a major concern because this arthroplasty has not solved the problem, and other solutions will have to be found.", "contents": "Guepar hinge prosthesis: complications and results with two years' follow-up. Experience with the GUEPAR prosthesis in 292 cases of which 103 have been followed for more than 2 years, suggests that: implanting a hinge prosthesis is major surgery on elderly patients in whom severe complications have occurred and for this reason, the operations should be reserved for extremely damaged and unstable knees; the most important local complications have been deep sepsis for which we have noted a rate of 6.6 per cent; in the treatment of sepsis, everything must be done to preserve the prosthesis because arthrodesis is difficult to obtain; pain relief has been significant as a result of the operation. The prosthetic design allows flexion of more than 90 degrees in 85 per cent of the cases and 120 degrees in 26 per cent; after two years, the results seem relatively stable. We have not observed aseptic loosening after this period but a longer observation period is necessary to be reassured on this point; patellar pain remains a major concern because this arthroplasty has not solved the problem, and other solutions will have to be found.", "PMID": 975666}
{"id": "pubmed23n0032_10417", "title": "Statistics of total knee replacement: partial and total knee replacement, design St. Georg: a review of a 4-year observation.", "content": "Since 1969, we have used 2 different types of knee prostheses to reconstruct arthritic knees. The surface replacement prosthesis (sledge) is indicated where ligamentous stability is present and angular deformity is not severe. In addition, the sledge prosthesis may be implanted with good results in selected acute comminuted tibial plateau fractures. The total hinged prosthesis of metal design is recommended in knees which are severely deformed, and unstable, and therefore not suitable for the sledge prosthesis. Marked relief of pain was the most significant result in our patients. Residual knee pain was most frequently due to patello-femoral pain and, when severe, this was satisfactorily controlled by performing a patellectomy. Motion was usually maintained at the preoperative range or improved, mainly by reducing or eliminating the knee flexion contracture. In a high percentage, the unicompartmental sledge prosthesis has given excellent results. However, we tend to insert a bi-compartmental sledge prosthesis because experience has shown that even slight damage of the articular surfaces of the opposite side is likely to further deteriorate rapidly. Although a significant number of complications occurred, these have been minimized by further conservative and operative treatment. We anticipate a further reduction in complications based on our initial experience, and prosthetic revisions.", "contents": "Statistics of total knee replacement: partial and total knee replacement, design St. Georg: a review of a 4-year observation. Since 1969, we have used 2 different types of knee prostheses to reconstruct arthritic knees. The surface replacement prosthesis (sledge) is indicated where ligamentous stability is present and angular deformity is not severe. In addition, the sledge prosthesis may be implanted with good results in selected acute comminuted tibial plateau fractures. The total hinged prosthesis of metal design is recommended in knees which are severely deformed, and unstable, and therefore not suitable for the sledge prosthesis. Marked relief of pain was the most significant result in our patients. Residual knee pain was most frequently due to patello-femoral pain and, when severe, this was satisfactorily controlled by performing a patellectomy. Motion was usually maintained at the preoperative range or improved, mainly by reducing or eliminating the knee flexion contracture. In a high percentage, the unicompartmental sledge prosthesis has given excellent results. However, we tend to insert a bi-compartmental sledge prosthesis because experience has shown that even slight damage of the articular surfaces of the opposite side is likely to further deteriorate rapidly. Although a significant number of complications occurred, these have been minimized by further conservative and operative treatment. We anticipate a further reduction in complications based on our initial experience, and prosthetic revisions.", "PMID": 975667}
{"id": "pubmed23n0032_10418", "title": "ICLH (Freeman/Swanson) arthroplasty in the treatment of arthritic knee: a 2 to 4-year review.", "content": "A prospective follow-up using a standardized data recording form was carried out by independent, experienced orthopedic surgeons on a small number (116) of knees operated upon at The London Hospital in the period January 1, 1971 to October 31, 1973. An additional 93 knees have been operated upon at other hospitals and followed prospectively using the same data recording system. A total of 159 knees have been reviewed 2 to 4 years after operation. The complications specific to ICLH arthroplasty have been unremarkable and generally correctable. Two tibial components have sunk and have been revised at The London Hospital. The results show that ICLH arthroplasty has been used to correct satisfactorily flexion deformities up to 70 degrees, valgus deformities up to 45 degrees, and varus deformities up to 35 degrees. (Deformities of greater magnitude can be corrected but were not present among the knees in this study.) The operation may be said to be \"worthwhile\" with reference to pain in 94 per cent of the knees, with reference to the ability to walk in 80 per cent of the knees, and with reference to the range of movement in 71 per cent. Overall, 47 per cent of the knees had no complaint postoperatively, while a further 47 per cent were improved; thus for 94 per cent the procedure was \"worthwhile.\"", "contents": "ICLH (Freeman/Swanson) arthroplasty in the treatment of arthritic knee: a 2 to 4-year review. A prospective follow-up using a standardized data recording form was carried out by independent, experienced orthopedic surgeons on a small number (116) of knees operated upon at The London Hospital in the period January 1, 1971 to October 31, 1973. An additional 93 knees have been operated upon at other hospitals and followed prospectively using the same data recording system. A total of 159 knees have been reviewed 2 to 4 years after operation. The complications specific to ICLH arthroplasty have been unremarkable and generally correctable. Two tibial components have sunk and have been revised at The London Hospital. The results show that ICLH arthroplasty has been used to correct satisfactorily flexion deformities up to 70 degrees, valgus deformities up to 45 degrees, and varus deformities up to 35 degrees. (Deformities of greater magnitude can be corrected but were not present among the knees in this study.) The operation may be said to be \"worthwhile\" with reference to pain in 94 per cent of the knees, with reference to the ability to walk in 80 per cent of the knees, and with reference to the range of movement in 71 per cent. Overall, 47 per cent of the knees had no complaint postoperatively, while a further 47 per cent were improved; thus for 94 per cent the procedure was \"worthwhile.\"", "PMID": 975668}
{"id": "pubmed23n0032_10419", "title": "Duo-condylar knee arthroplasty: hospital for special surgery design.", "content": "This is a report of 94 knees in 88 patients with the duo-condylar type of knee arthroplasty. The follow-up period of time was between 2 to 4 years with an average of 3 years. The rheumatoid to osteoarthritic patient ratio was 3 to 1. The overall results were excellent in 37.5 per cent, good in 37.5 per cent, fair in 16 per cent, and poor in 9 per cent. The main causes of failure and poor results were: (1) under or over correction of deformity leading to subluxation and/or instability of the knee; (2) loosening of the tibial component, and (3) symptoms arising from the patellofemoral joint. The revision rate is 5.5 per cent. The progressive radiolucency at the cement bone bond is 26 per cent of which 16 per cent is up to 1 mm and 10 per cent is between 1.5 to 3 mm. To further improve the results of arthroplasty, one should take into consideration (1) replacement of the patellofemoral joint, (2) insertion of the prosthesis in the proper anatomical location under correct tension of the ligaments and capsule with the help of proper instrumentation and (3) improvement in fixation of the tibial component.", "contents": "Duo-condylar knee arthroplasty: hospital for special surgery design. This is a report of 94 knees in 88 patients with the duo-condylar type of knee arthroplasty. The follow-up period of time was between 2 to 4 years with an average of 3 years. The rheumatoid to osteoarthritic patient ratio was 3 to 1. The overall results were excellent in 37.5 per cent, good in 37.5 per cent, fair in 16 per cent, and poor in 9 per cent. The main causes of failure and poor results were: (1) under or over correction of deformity leading to subluxation and/or instability of the knee; (2) loosening of the tibial component, and (3) symptoms arising from the patellofemoral joint. The revision rate is 5.5 per cent. The progressive radiolucency at the cement bone bond is 26 per cent of which 16 per cent is up to 1 mm and 10 per cent is between 1.5 to 3 mm. To further improve the results of arthroplasty, one should take into consideration (1) replacement of the patellofemoral joint, (2) insertion of the prosthesis in the proper anatomical location under correct tension of the ligaments and capsule with the help of proper instrumentation and (3) improvement in fixation of the tibial component.", "PMID": 975669}
{"id": "pubmed23n0032_10420", "title": "Unicondylar knee replacement.", "content": "Unicompartmental knee arthroplasty was conceived for the treatment of osteoarthritic knees with deterioration of either the medial or lateral compartment of the knee. The Unicondylar prosthesis was used in 19 medial and 5 lateral compartment arthroplasties. A two to 4 year follow-up evaluation of these patients was somewhat disappointing as only 5 knees could be rated excellent and 6 rated a good result. The best results were seen in the lateral compartment arthroplasties. Such deformities may be the only future indication for the use of this operation as these knees do not do well when treated by tibial osteotomy. However, when only the medial compartment is involved, osteotomy may still remain the treatment of choice.", "contents": "Unicondylar knee replacement. Unicompartmental knee arthroplasty was conceived for the treatment of osteoarthritic knees with deterioration of either the medial or lateral compartment of the knee. The Unicondylar prosthesis was used in 19 medial and 5 lateral compartment arthroplasties. A two to 4 year follow-up evaluation of these patients was somewhat disappointing as only 5 knees could be rated excellent and 6 rated a good result. The best results were seen in the lateral compartment arthroplasties. Such deformities may be the only future indication for the use of this operation as these knees do not do well when treated by tibial osteotomy. However, when only the medial compartment is involved, osteotomy may still remain the treatment of choice.", "PMID": 975670}
{"id": "pubmed23n0032_10421", "title": "The Modular (Marmor) knee: case report with a minimum follow-up of 2 years.", "content": "A follow-up of 2 years or more on 105 patients with the Modular (Marmor) knee replacement revealed that 88 per cent of the patients had a successful result. The complications and failures are analyzed in depth. Late loosening of the components was not observed except with the 6 mm tibial plateau. Pain relief was dramatic as well as improved function, stability and motion.", "contents": "The Modular (Marmor) knee: case report with a minimum follow-up of 2 years. A follow-up of 2 years or more on 105 patients with the Modular (Marmor) knee replacement revealed that 88 per cent of the patients had a successful result. The complications and failures are analyzed in depth. Late loosening of the components was not observed except with the 6 mm tibial plateau. Pain relief was dramatic as well as improved function, stability and motion.", "PMID": 975671}
{"id": "pubmed23n0032_10422", "title": "Hematological and vascular concepts in relation to stroke.", "content": "This review has highlighted some of the factors which influence the formation and fate of thrombi. Rheology and flow, the coagulation mechanism, the fibrinolytic enzyme system, and the properties of platelets to adhere and aggregate all play a significant role. Of paramount importance to neurosurgeons is the role of the vascular wall in thrombogenesis. The ulcerated atherosclerotic plaque demonstrates this point. Factors contributing to thromboembolism in this setting include stenosis and reduced flow, turbulence, and the elements in the lesion with which blood interacts. These are principally collagen, ADP, fatty acids, and the thromboplastic activity of vascular subendothelial tissue. Current concepts of the etiologies of atherosclerosis were discussed. Along with other predisposing factors, platelets may play a significant role in the initiation of the process. The current status of clinical trials with inhibitors of platelet function in the treatment of cerebrovascular disorders was reviewed. In vascular reconstructive surgery, the principles which underlie good technique are those which best insure against thrombosis. The briefest possible period of stasis during surgery should be maintained. The reconstruction should result in a smooth blood flow pattern with rapid runoff and minimal stenosis or gaps between apposed endothelial surfaces.", "contents": "Hematological and vascular concepts in relation to stroke. This review has highlighted some of the factors which influence the formation and fate of thrombi. Rheology and flow, the coagulation mechanism, the fibrinolytic enzyme system, and the properties of platelets to adhere and aggregate all play a significant role. Of paramount importance to neurosurgeons is the role of the vascular wall in thrombogenesis. The ulcerated atherosclerotic plaque demonstrates this point. Factors contributing to thromboembolism in this setting include stenosis and reduced flow, turbulence, and the elements in the lesion with which blood interacts. These are principally collagen, ADP, fatty acids, and the thromboplastic activity of vascular subendothelial tissue. Current concepts of the etiologies of atherosclerosis were discussed. Along with other predisposing factors, platelets may play a significant role in the initiation of the process. The current status of clinical trials with inhibitors of platelet function in the treatment of cerebrovascular disorders was reviewed. In vascular reconstructive surgery, the principles which underlie good technique are those which best insure against thrombosis. The briefest possible period of stasis during surgery should be maintained. The reconstruction should result in a smooth blood flow pattern with rapid runoff and minimal stenosis or gaps between apposed endothelial surfaces.", "PMID": 975674}
{"id": "pubmed23n0032_10423", "title": "Host--dose--time: a concept for atherosclerosis.", "content": "Advances in knowledge about atherosclerosis in the past 25 years were discussed. Areas of disagreement and agreement were identified. A concept of HOST-DOSE-TIME was introduced for the skeptical analysis of past, present, and future observation and treatment of atherosclerosis.", "contents": "Host--dose--time: a concept for atherosclerosis. Advances in knowledge about atherosclerosis in the past 25 years were discussed. Areas of disagreement and agreement were identified. A concept of HOST-DOSE-TIME was introduced for the skeptical analysis of past, present, and future observation and treatment of atherosclerosis.", "PMID": 975675}
{"id": "pubmed23n0032_10424", "title": "Cardiogenic factors in the stroke syndrome.", "content": "The cardiogenic factors which may contribute to the stroke syndrome have been discussed (Table 14.7). Pathophysiological mechanisms include emboli from rheumatic coronary and congenital heart diseases. Atherosclerotic disease and hypertension can involve both intracranial and extracranial arteries. Disturbances in cardiac rhythm can significantly reduce cerebral blood flow. The development of cerebral hypoxia with resuscitation is described. Treatment of these disorders is reviewed, and the potential role of metabolic support of the ischemic tissue both with cerebral and myocardial damage has been presented.", "contents": "Cardiogenic factors in the stroke syndrome. The cardiogenic factors which may contribute to the stroke syndrome have been discussed (Table 14.7). Pathophysiological mechanisms include emboli from rheumatic coronary and congenital heart diseases. Atherosclerotic disease and hypertension can involve both intracranial and extracranial arteries. Disturbances in cardiac rhythm can significantly reduce cerebral blood flow. The development of cerebral hypoxia with resuscitation is described. Treatment of these disorders is reviewed, and the potential role of metabolic support of the ischemic tissue both with cerebral and myocardial damage has been presented.", "PMID": 975677}
{"id": "pubmed23n0032_10425", "title": "The radionuclide cerebral angiogram in stroke diagnosis.", "content": "The radionuclide cerebral angiogram using intravenous radiopertechnetate has been found to be of value in confirming the early diagnosis of stroke. The isotopic perfusion study is of value in detecting ischemic carotid and middle cerebral lesions. It can also provide a rapid means of demonstrating intracerebral hemorrhages. Nuclear angiography is not recommended for detection of vertebrobasilar artery disease unless a posterior cerebral artery occlusion is suspected.", "contents": "The radionuclide cerebral angiogram in stroke diagnosis. The radionuclide cerebral angiogram using intravenous radiopertechnetate has been found to be of value in confirming the early diagnosis of stroke. The isotopic perfusion study is of value in detecting ischemic carotid and middle cerebral lesions. It can also provide a rapid means of demonstrating intracerebral hemorrhages. Nuclear angiography is not recommended for detection of vertebrobasilar artery disease unless a posterior cerebral artery occlusion is suspected.", "PMID": 975680}
{"id": "pubmed23n0032_10426", "title": "Shunt complications.", "content": "To some extent our greatest problem is the philosophic answer to the question, \"who shall be treated, how intensely and to what expense?\" The cost of care is almost always greatest in the child who is borderline in selection and least likely to survive. Over half of the children shunted after application of searching criteria appear to this author to be compensated (Table 28.13).", "contents": "Shunt complications. To some extent our greatest problem is the philosophic answer to the question, \"who shall be treated, how intensely and to what expense?\" The cost of care is almost always greatest in the child who is borderline in selection and least likely to survive. Over half of the children shunted after application of searching criteria appear to this author to be compensated (Table 28.13).", "PMID": 975690}
{"id": "pubmed23n0032_10427", "title": "The role of vascular proliferation in the growth of brain tumors.", "content": "The formation of new blood vessels, i.e., angiogenesis, is a critical step in the evolution of a brain tumor from the earliest avascular phase to the clinically overt, vascular stage. For cerebral astrocytomas, intense capillary proliferation is a poor prognostic sign, associated with rapidly growing tumors. The in vivo production of a vasoformative substance can be demonstrated by transplanting human and experimental gliomas to the rabbit cornea. Intense neovascularization is elicited by the tumor and not by control tissues. If the same tumor is transplanted to the vitreous, thereby interrupting the vasoformative signal, the tumor remains in a dormant state for a prolonged period of time, despite its malignant potential. Inhibition of angiogenesis would be an important adjunct to the surgical treatment of gliomas.", "contents": "The role of vascular proliferation in the growth of brain tumors. The formation of new blood vessels, i.e., angiogenesis, is a critical step in the evolution of a brain tumor from the earliest avascular phase to the clinically overt, vascular stage. For cerebral astrocytomas, intense capillary proliferation is a poor prognostic sign, associated with rapidly growing tumors. The in vivo production of a vasoformative substance can be demonstrated by transplanting human and experimental gliomas to the rabbit cornea. Intense neovascularization is elicited by the tumor and not by control tissues. If the same tumor is transplanted to the vitreous, thereby interrupting the vasoformative signal, the tumor remains in a dormant state for a prolonged period of time, despite its malignant potential. Inhibition of angiogenesis would be an important adjunct to the surgical treatment of gliomas.", "PMID": 975695}
{"id": "pubmed23n0032_10428", "title": "Complications of percutaneous surgery for pain.", "content": "Percutaneous procedures are proving themselves to be useful additions to the group of neurosurgical procedures for treating pain. They demand a different style of careful operating technique and they place heavy reliance on the gradual production of lesions to achieve the desired degree of denervation. Control of lesion position and extent through further advances in neurophysiological monitoring should give these procedures an even wider margin of safety and reliability.", "contents": "Complications of percutaneous surgery for pain. Percutaneous procedures are proving themselves to be useful additions to the group of neurosurgical procedures for treating pain. They demand a different style of careful operating technique and they place heavy reliance on the gradual production of lesions to achieve the desired degree of denervation. Control of lesion position and extent through further advances in neurophysiological monitoring should give these procedures an even wider margin of safety and reliability.", "PMID": 975696}
{"id": "pubmed23n0032_10429", "title": "Complications of surgery for subdural hematoma.", "content": "In this report we have discussed the complications of surgery for subdural hematoma. The complications are varied and many depend primarily on the type of treatment required by the patient. We have considered the problems associated with: percutaneous tap in infants, subdural shunts in infants and children, craniotomy for membranectomy, twist-drill drainage, and burr holes, craneictomy, and/or craniotomy. Infection, epilepsy, and reaccumulation of the subdural hematoma are common to all forms of therapy, while other complications are related to the specific form of subdural hematoma treatment. In addition, significant serum and electrolyte abnormalities which may occur as a consequence of subdural hematoma surgery have been reviewed. In general, the over-all complication rate of surgery for subdural hematoma is low in relationship to the morbidity and mortality associated with this lesion. Because of the frequency of subdural hematoma, the neurosurgeon should be ever vigilant in the prevention, recognition, and management of these complications.", "contents": "Complications of surgery for subdural hematoma. In this report we have discussed the complications of surgery for subdural hematoma. The complications are varied and many depend primarily on the type of treatment required by the patient. We have considered the problems associated with: percutaneous tap in infants, subdural shunts in infants and children, craniotomy for membranectomy, twist-drill drainage, and burr holes, craneictomy, and/or craniotomy. Infection, epilepsy, and reaccumulation of the subdural hematoma are common to all forms of therapy, while other complications are related to the specific form of subdural hematoma treatment. In addition, significant serum and electrolyte abnormalities which may occur as a consequence of subdural hematoma surgery have been reviewed. In general, the over-all complication rate of surgery for subdural hematoma is low in relationship to the morbidity and mortality associated with this lesion. Because of the frequency of subdural hematoma, the neurosurgeon should be ever vigilant in the prevention, recognition, and management of these complications.", "PMID": 975697}
{"id": "pubmed23n0032_10430", "title": "ICP monitoring: complications and associated factors.", "content": "1. Patients with complications when compared with those not developing a complication: a. Were older, 44.4 vs. 34.5 years; b. Were monitored longer, 14.0 vs. 6.5 days; c. Were treated with steroids longer, 18.3 vs. 10.3 days; d. Were hospitalized longer, 35.7 vs. 27.7 days; e. Are twice as likely to die; f. Average age of those dying is older, 46 vs. 30 years; g. Have no clear relationship to antibiotics; h. Do not have a preponderance of single diagnostic category to account for these differences; i. Complications were always controlled with standard therapy; j. An ICP complication was almost never responsible, per se, for death. 2. Over all complication rate was 18%; due to monitoring was 4.5 to 11.5%. 3. Monitoring was responsible for, or contributed to, 25 to 60% of the complications. 4. No complications occurred when monitoring was discontinued within 3 days. 5. Long periods of treatment with steroids increased the risk of a complication developing. 6. Long periods of monitoring were associated with an increased risk of complication. 7. \"Individual\" factors are partly responsible for the development of a complication, and death. 8. There are two groups of complications: \"early\" and \"late.\" A. Those most likely to be monitor related occur \"late\" and account for 35% of the complications. B. Those developing \"early\" infection account for 65% of the complications and are most likely caused by factors other than monitoring. While there are complications directly attributable to monitoring, the rate is low and they are readily controlled by standard neurosurgical management. While monitoring may increase the morbidity, it does not increase the mortality. We feel that the over-all risk is small and that the returns are great--both in terms of knowledge and patient benefit.", "contents": "ICP monitoring: complications and associated factors. 1. Patients with complications when compared with those not developing a complication: a. Were older, 44.4 vs. 34.5 years; b. Were monitored longer, 14.0 vs. 6.5 days; c. Were treated with steroids longer, 18.3 vs. 10.3 days; d. Were hospitalized longer, 35.7 vs. 27.7 days; e. Are twice as likely to die; f. Average age of those dying is older, 46 vs. 30 years; g. Have no clear relationship to antibiotics; h. Do not have a preponderance of single diagnostic category to account for these differences; i. Complications were always controlled with standard therapy; j. An ICP complication was almost never responsible, per se, for death. 2. Over all complication rate was 18%; due to monitoring was 4.5 to 11.5%. 3. Monitoring was responsible for, or contributed to, 25 to 60% of the complications. 4. No complications occurred when monitoring was discontinued within 3 days. 5. Long periods of treatment with steroids increased the risk of a complication developing. 6. Long periods of monitoring were associated with an increased risk of complication. 7. \"Individual\" factors are partly responsible for the development of a complication, and death. 8. There are two groups of complications: \"early\" and \"late.\" A. Those most likely to be monitor related occur \"late\" and account for 35% of the complications. B. Those developing \"early\" infection account for 65% of the complications and are most likely caused by factors other than monitoring. While there are complications directly attributable to monitoring, the rate is low and they are readily controlled by standard neurosurgical management. While monitoring may increase the morbidity, it does not increase the mortality. We feel that the over-all risk is small and that the returns are great--both in terms of knowledge and patient benefit.", "PMID": 975699}
{"id": "pubmed23n0032_10431", "title": "Radical surgical treatment of craniopharyngioma.", "content": "There is evidence from this publication and those of Matson and colleagues that a determined effort at total excision of these tumors as the initial therapy is a tenable course of action. The main bases for this concept are: (1) that the dense gliosis characteristically intervening between these epithelial tumors and normal brain constitutes a margin of safety for the surgeon at least for the first several years such tumors are growing in the 3rd ventricle; (2) improved early diagnosis and better operative instruments, magnification, lighting, and technique are decreasing operative morbidity and mortality; and (3) new knowledge and new replacement therapy are reducing the burden of the metabolic and endocrine defects. Competing with this is the concept that rotationally delivered or even more precisely focused high energy photons and intracavitary beta-radiation have lower morbidity and mortality. The detailed and long term data on which to draw conclusions are not yet available. My medical colleagues and I are preparing case-by-case tables with all of the relevant facts to supply one component of the total picture.", "contents": "Radical surgical treatment of craniopharyngioma. There is evidence from this publication and those of Matson and colleagues that a determined effort at total excision of these tumors as the initial therapy is a tenable course of action. The main bases for this concept are: (1) that the dense gliosis characteristically intervening between these epithelial tumors and normal brain constitutes a margin of safety for the surgeon at least for the first several years such tumors are growing in the 3rd ventricle; (2) improved early diagnosis and better operative instruments, magnification, lighting, and technique are decreasing operative morbidity and mortality; and (3) new knowledge and new replacement therapy are reducing the burden of the metabolic and endocrine defects. Competing with this is the concept that rotationally delivered or even more precisely focused high energy photons and intracavitary beta-radiation have lower morbidity and mortality. The detailed and long term data on which to draw conclusions are not yet available. My medical colleagues and I are preparing case-by-case tables with all of the relevant facts to supply one component of the total picture.", "PMID": 975700}
{"id": "pubmed23n0032_10432", "title": "Controlled thermocoagulation of trigeminal ganglion and rootlets for differential destruction of pain fibers: facial pain other than trigeminal neuralgia.", "content": "I describe the results of retrogasserian differential lidocaine block to aid in the selection of patients for a differential thermal lesion in the trigeminal ganglion and rootlets. This procedure temporarily duplicates the state of analgesia without anesthesia one seeks to make permanent with the radiofrequency heating. The results of this heating procedure are described in the treatment of 71 patients with facial pain of cancer, postherpetic, periodic migrainous neuralgia, acromegaly, trigeminal neuropathy, central pain, post-traumatic facial neuralgia, and atypical facial neuralgia.", "contents": "Controlled thermocoagulation of trigeminal ganglion and rootlets for differential destruction of pain fibers: facial pain other than trigeminal neuralgia. I describe the results of retrogasserian differential lidocaine block to aid in the selection of patients for a differential thermal lesion in the trigeminal ganglion and rootlets. This procedure temporarily duplicates the state of analgesia without anesthesia one seeks to make permanent with the radiofrequency heating. The results of this heating procedure are described in the treatment of 71 patients with facial pain of cancer, postherpetic, periodic migrainous neuralgia, acromegaly, trigeminal neuropathy, central pain, post-traumatic facial neuralgia, and atypical facial neuralgia.", "PMID": 975705}
{"id": "pubmed23n0032_10433", "title": "The prognostic significance of fever in childhood urinary infections: observations in 350 consecutive patients.", "content": "All infants and children with proven bacteriuria require urologic investigation and surveillance, but their prospects for developing chronic renal disease are variable. These differences are related to whether or not the bacteria have access to the kidneys. Such access correlates closely with the presence of an underlying congenital anomaly, particularly vesicoureteral reflux. Fever during an episode of active urinary infection is a strong indication that the patient has vesicoureteral reflux or some other significant uropathy and probably pyelonephritis.", "contents": "The prognostic significance of fever in childhood urinary infections: observations in 350 consecutive patients. All infants and children with proven bacteriuria require urologic investigation and surveillance, but their prospects for developing chronic renal disease are variable. These differences are related to whether or not the bacteria have access to the kidneys. Such access correlates closely with the presence of an underlying congenital anomaly, particularly vesicoureteral reflux. Fever during an episode of active urinary infection is a strong indication that the patient has vesicoureteral reflux or some other significant uropathy and probably pyelonephritis.", "PMID": 975708}
{"id": "pubmed23n0032_10434", "title": "Tuberculosis among urban black children: failure of comprehensive health care services to influence incidence rates.", "content": "Over a three-year period, in 2,700 children aged two weeks to 19 years in a South Philadelphia ghetto, the new case rate for tuberculosis was 177 per 100,000 per year and the rate for new conversions was 673 per 100,000 per year. Children with recent conversions or with active disease were, on the average, slightly older and had older mothers and more adults and siblings at home than the rest of the child population. Parents or other residents of the home were the contacts. Children 10 to 14 years of age were the most frequently affected. Children who had been receiving continuous comprehensive health care for more than three years had the same attack rates as new children in the program.", "contents": "Tuberculosis among urban black children: failure of comprehensive health care services to influence incidence rates. Over a three-year period, in 2,700 children aged two weeks to 19 years in a South Philadelphia ghetto, the new case rate for tuberculosis was 177 per 100,000 per year and the rate for new conversions was 673 per 100,000 per year. Children with recent conversions or with active disease were, on the average, slightly older and had older mothers and more adults and siblings at home than the rest of the child population. Parents or other residents of the home were the contacts. Children 10 to 14 years of age were the most frequently affected. Children who had been receiving continuous comprehensive health care for more than three years had the same attack rates as new children in the program.", "PMID": 975709}
{"id": "pubmed23n0032_10435", "title": "Effect of furosemide on the renal excretion of digoxin.", "content": "Digoxin serum and urine levels were determined by radioimmunoassay in 6 subjects (4 patients with heart disease and 2 volunteers without heart disease) who had been maintained on oral digoxin (0.25 or 0.5 mg daily). Observations were made during a 3-day control period and then during 8 days of concomitant digoxin and oral furosemide (40 mg daily) therapy. Serum digoxin levels determined 10 and 24 hr after each dose of digoxin averaged 1.2+/-0.1 ng/ml (M+/-SE) during control and 1.3+/-0.1 during the last 3 days on digoxin and furosemide. The daily urinary excretion of digoxine averaged 51+/-6% of the oral dose during control and 52+/-6 during the entire period of furosemide administration. The renal clearance of digoxin and creatinine averaged 94+/-7 and 87+/-11 ml/min, respectively, during control; corresponding values were 88+/-8 and 85+/-9 for urine collections demonstrating a distinct diuretic effect of furosemide and 87+/-8 and 75+/-10 for urine collections not demonstrating such an effect during diuretic therapy. The results suggest that the diuretic effect of furosemide does not significantly affect the excretion of digoxin", "contents": "Effect of furosemide on the renal excretion of digoxin. Digoxin serum and urine levels were determined by radioimmunoassay in 6 subjects (4 patients with heart disease and 2 volunteers without heart disease) who had been maintained on oral digoxin (0.25 or 0.5 mg daily). Observations were made during a 3-day control period and then during 8 days of concomitant digoxin and oral furosemide (40 mg daily) therapy. Serum digoxin levels determined 10 and 24 hr after each dose of digoxin averaged 1.2+/-0.1 ng/ml (M+/-SE) during control and 1.3+/-0.1 during the last 3 days on digoxin and furosemide. The daily urinary excretion of digoxine averaged 51+/-6% of the oral dose during control and 52+/-6 during the entire period of furosemide administration. The renal clearance of digoxin and creatinine averaged 94+/-7 and 87+/-11 ml/min, respectively, during control; corresponding values were 88+/-8 and 85+/-9 for urine collections demonstrating a distinct diuretic effect of furosemide and 87+/-8 and 75+/-10 for urine collections not demonstrating such an effect during diuretic therapy. The results suggest that the diuretic effect of furosemide does not significantly affect the excretion of digoxin", "PMID": 975715}
{"id": "pubmed23n0032_10436", "title": "Renal failure, hemodialysis, and nafcillin kinetics.", "content": "Nafcillin (N) pharmacokinetics was studied in 27 subjectswith and without renal failure (RF) (determined by endogenous creatinine clearance, Ccr). Elimination rate constants (K) were calculated from serial serum levels of N measured from 2 to 12 hr after a single 500-mg intramuscular injection. Only 4 of 9 hemodialysis patients had measurable levels of N at 24 hr. The K values for the groups with normal renal function,moderate RF, severe RF, and on hemodialysis were 0.477 hr(-1), 0.432 hr (1), 0.369 hr(-1), and 0.306 hr (-1), respectively...", "contents": "Renal failure, hemodialysis, and nafcillin kinetics. Nafcillin (N) pharmacokinetics was studied in 27 subjectswith and without renal failure (RF) (determined by endogenous creatinine clearance, Ccr). Elimination rate constants (K) were calculated from serial serum levels of N measured from 2 to 12 hr after a single 500-mg intramuscular injection. Only 4 of 9 hemodialysis patients had measurable levels of N at 24 hr. The K values for the groups with normal renal function,moderate RF, severe RF, and on hemodialysis were 0.477 hr(-1), 0.432 hr (1), 0.369 hr(-1), and 0.306 hr (-1), respectively...", "PMID": 975716}
{"id": "pubmed23n0032_10437", "title": "Amobarbital--a probe of hepatic drug oxidation in man.", "content": "Some aspects of the fate of amobarbital were investigated since this drug is being used as a probe to gauge drug oxidation in man. The mean ratio of orally available over intravenously injected amobarbital was established as 0.99 +/- 0.11 (SD), by comparing integrated concentration-time curves, indicating complete absorption and absence of a first-pass effect. One subject ingested 200 mg of amobarbital sodium, and amobarbital concentrations in serum were monitored for 5 days thereafter. Elimination of amobarbital under these conditions followed first-order kinetics. One subject ingested amobarbital 7 times over a period of 3 yr; plasma clearances (32.1 +/- 1.8 [SD]ml/min) exhibited remarkable constancy, while biologic half-lives (26.5 +/- 3.1 hr) and distribution volumes (73.6 +/- 8.0 L) showed some fluctuation. The distribution of parameters of amobarbital elimination was investigated in 36 unrelated subjects. Amobarbital half-lives (23.8 +/- 6.7 hr) appeared to be normally distributed, while the clearances (36.7 +/- 10.0 ml/min) might not follow a normal distribution.", "contents": "Amobarbital--a probe of hepatic drug oxidation in man. Some aspects of the fate of amobarbital were investigated since this drug is being used as a probe to gauge drug oxidation in man. The mean ratio of orally available over intravenously injected amobarbital was established as 0.99 +/- 0.11 (SD), by comparing integrated concentration-time curves, indicating complete absorption and absence of a first-pass effect. One subject ingested 200 mg of amobarbital sodium, and amobarbital concentrations in serum were monitored for 5 days thereafter. Elimination of amobarbital under these conditions followed first-order kinetics. One subject ingested amobarbital 7 times over a period of 3 yr; plasma clearances (32.1 +/- 1.8 [SD]ml/min) exhibited remarkable constancy, while biologic half-lives (26.5 +/- 3.1 hr) and distribution volumes (73.6 +/- 8.0 L) showed some fluctuation. The distribution of parameters of amobarbital elimination was investigated in 36 unrelated subjects. Amobarbital half-lives (23.8 +/- 6.7 hr) appeared to be normally distributed, while the clearances (36.7 +/- 10.0 ml/min) might not follow a normal distribution.", "PMID": 975717}
{"id": "pubmed23n0032_10438", "title": "Protein binding of salicylate in uremic and normal plasma.", "content": "Protein binding of salicylate was studied by equilibrium dialysis at 37 degrees C in plasma from uremic patients and healthy subjects. The protein binding was considerably lower in the uremic plasma at all salicylate concentrations studied (14 to 1,400 mug/ml). Scatchard plots of the data were computer-analyzed assuming binding to the classes of binding sites. According to this analysis, the binding to the class of primary binding sites was considerably decreased in the uremic plasma. In addition to the effect of the uremic state, the binding was considerably decreased at high therapeutic plasma levels and at low albumin levels. The combined effect of two or three of these factors may lead to unexpectedly high unbound fractions of salicylate, which should be considered in the monitoring of plasma salicylate levels in patients.", "contents": "Protein binding of salicylate in uremic and normal plasma. Protein binding of salicylate was studied by equilibrium dialysis at 37 degrees C in plasma from uremic patients and healthy subjects. The protein binding was considerably lower in the uremic plasma at all salicylate concentrations studied (14 to 1,400 mug/ml). Scatchard plots of the data were computer-analyzed assuming binding to the classes of binding sites. According to this analysis, the binding to the class of primary binding sites was considerably decreased in the uremic plasma. In addition to the effect of the uremic state, the binding was considerably decreased at high therapeutic plasma levels and at low albumin levels. The combined effect of two or three of these factors may lead to unexpectedly high unbound fractions of salicylate, which should be considered in the monitoring of plasma salicylate levels in patients.", "PMID": 975718}
{"id": "pubmed23n0032_10439", "title": "Antipyrin clearance in homozygous beta-thalassemia.", "content": "Serum half-lives for antipyrine were normal or shorter than normal in 19 subjects between 7 and 23 yr of age with beta-thalassemia major. The mean antipyrine serum half life (+/-SE) for the group as a whole was 8.5 +/- 0.6 hr. The mean antipyrine half-lives (t1/2) for the younger subjects were within the range reported for normal children, while the mean t1/2 for the older males approached the values reported for normal adult males. The mean t1/2 for the older females was shorter than has been reported for normal adult females. The mean apparent volume of distribution for antipyrine (+/-SE) in the subjects with thalassemia was 0.69 +/- 0.01 L/kg. Thus, total body water appears to be increased in thalassemia. The mean metabolic clearance rate for antipyrine (+/-SE) in the group as a whole (1.07 +/- 0.08 ml/min/kg) is substantially higher than the metabolic clearance rates for antipyrine reported in normal adults. Thus, the relatively short t1/2s of antipyrine in subjects with thalassemia are attributable to rapid rates of clearance of the drug. The data indicate that antipyrine clearance is unimpaired in patients with thalassemia despite evidence of liver damage and iron overload. Our study supports the proposition that hepatic microsomal hemoprotein synthesis is not adversely affected in homozygous beta-thalassemia.", "contents": "Antipyrin clearance in homozygous beta-thalassemia. Serum half-lives for antipyrine were normal or shorter than normal in 19 subjects between 7 and 23 yr of age with beta-thalassemia major. The mean antipyrine serum half life (+/-SE) for the group as a whole was 8.5 +/- 0.6 hr. The mean antipyrine half-lives (t1/2) for the younger subjects were within the range reported for normal children, while the mean t1/2 for the older males approached the values reported for normal adult males. The mean t1/2 for the older females was shorter than has been reported for normal adult females. The mean apparent volume of distribution for antipyrine (+/-SE) in the subjects with thalassemia was 0.69 +/- 0.01 L/kg. Thus, total body water appears to be increased in thalassemia. The mean metabolic clearance rate for antipyrine (+/-SE) in the group as a whole (1.07 +/- 0.08 ml/min/kg) is substantially higher than the metabolic clearance rates for antipyrine reported in normal adults. Thus, the relatively short t1/2s of antipyrine in subjects with thalassemia are attributable to rapid rates of clearance of the drug. The data indicate that antipyrine clearance is unimpaired in patients with thalassemia despite evidence of liver damage and iron overload. Our study supports the proposition that hepatic microsomal hemoprotein synthesis is not adversely affected in homozygous beta-thalassemia.", "PMID": 975719}
{"id": "pubmed23n0032_10440", "title": "Aminopyrine demethylation measured by breath analysis in cirrhosis.", "content": "The method of measuring the rate of aminopyrine demethylation by breath analysis was assessed in 23 normal subjects and 20 patients with cirrhosis. Carbon 14 aminopyrine specifically labeled at the two N-methyl groups was administered by mouth in a dose of 9 mg/kg, including a total radioactivity of 2 muCi. The decay of the specific activity of 14CO2 in breath (kb) was found to correlate (r = 0.91) with the disappearance of aminopyrine from plasma (KP). In normal volunteers, kb was 22.4%/hr; in patients with alcoholic and nonalcoholic cirrhosis it was depressed to 8.4%/hr (p less than 0.001). The degree of functional impairment found with the breath test was similar to the sulfobromophthalein (BSP) disappearance curve and the galactose elimination capacity. Although many questions relating to the aminopyrine breath test remain open, our data confirm and extend previous studies of 14CO2 breath analysis after 14C-aminopyrine administration. It is concluded that it represents a simple and noninvasive procedure which quantitatively reflects the microsomal function of the cirrhotic liver.", "contents": "Aminopyrine demethylation measured by breath analysis in cirrhosis. The method of measuring the rate of aminopyrine demethylation by breath analysis was assessed in 23 normal subjects and 20 patients with cirrhosis. Carbon 14 aminopyrine specifically labeled at the two N-methyl groups was administered by mouth in a dose of 9 mg/kg, including a total radioactivity of 2 muCi. The decay of the specific activity of 14CO2 in breath (kb) was found to correlate (r = 0.91) with the disappearance of aminopyrine from plasma (KP). In normal volunteers, kb was 22.4%/hr; in patients with alcoholic and nonalcoholic cirrhosis it was depressed to 8.4%/hr (p less than 0.001). The degree of functional impairment found with the breath test was similar to the sulfobromophthalein (BSP) disappearance curve and the galactose elimination capacity. Although many questions relating to the aminopyrine breath test remain open, our data confirm and extend previous studies of 14CO2 breath analysis after 14C-aminopyrine administration. It is concluded that it represents a simple and noninvasive procedure which quantitatively reflects the microsomal function of the cirrhotic liver.", "PMID": 975720}
{"id": "pubmed23n0032_10441", "title": "Pharmacokinetics of succinylcholine in infants, children, and adults.", "content": "The elimination of succinylcholine appears to follow first-order kinetics with a linear relationship between intensity of effect and logarithm of the dose. Determination of the time-course of neuromuscular blockade following succinylcholine in infants and children allowed us to compare the rate of recovery and elimination rate constant with those determined by others in adults. The rate of recovery was independent of dose for each age group. The rate of recovery was faster in children than in infants; the rate of recovery of infants was faster than that of adults. The elimination rate constant for infants was similar to that of children; for both the infants and children the rate constants appeared dissimilar from those of adults.", "contents": "Pharmacokinetics of succinylcholine in infants, children, and adults. The elimination of succinylcholine appears to follow first-order kinetics with a linear relationship between intensity of effect and logarithm of the dose. Determination of the time-course of neuromuscular blockade following succinylcholine in infants and children allowed us to compare the rate of recovery and elimination rate constant with those determined by others in adults. The rate of recovery was independent of dose for each age group. The rate of recovery was faster in children than in infants; the rate of recovery of infants was faster than that of adults. The elimination rate constant for infants was similar to that of children; for both the infants and children the rate constants appeared dissimilar from those of adults.", "PMID": 975721}
{"id": "pubmed23n0032_10442", "title": "Intravenous propranolol administration: a method for rapidly achieving and sustaining desired plasma levels.", "content": "A model for the intravenous administration of propranolol by a bolus-infusion technique designed to rapidly produce, then maintain, predicted plasma drug concentrations was derived from elimination kinetics in single-dose studies. Prospective testing of this model in 6 adult male subjects revealed a close correlation between predicted and observed drug levels; desired plasma concentrations were achieved within 5 min and maintained over the 30-min study period. By subtracting previously given bolus doses from the dose calculated as needed to produce a desired plasma level, progressive increases in predicted propranolol levels could be effected, with apparent maintenance of equilibrium. Correlations between the bolus doses and infusion rates and the plasma drug levels were consistent and significant, and constitute nomograms from which the dose of drug required to produce a desired plasma level may be approximated. The clearance of propranolol declined slightly as the drug plasma level increased, but did not significantly affect the accuracy of the model.", "contents": "Intravenous propranolol administration: a method for rapidly achieving and sustaining desired plasma levels. A model for the intravenous administration of propranolol by a bolus-infusion technique designed to rapidly produce, then maintain, predicted plasma drug concentrations was derived from elimination kinetics in single-dose studies. Prospective testing of this model in 6 adult male subjects revealed a close correlation between predicted and observed drug levels; desired plasma concentrations were achieved within 5 min and maintained over the 30-min study period. By subtracting previously given bolus doses from the dose calculated as needed to produce a desired plasma level, progressive increases in predicted propranolol levels could be effected, with apparent maintenance of equilibrium. Correlations between the bolus doses and infusion rates and the plasma drug levels were consistent and significant, and constitute nomograms from which the dose of drug required to produce a desired plasma level may be approximated. The clearance of propranolol declined slightly as the drug plasma level increased, but did not significantly affect the accuracy of the model.", "PMID": 975723}
{"id": "pubmed23n0032_10443", "title": "Systemic availability of orally administered meperidine.", "content": "A comparison of the areas under the plasma concentration-time curves after intravenous and oral administration of meperidine in 4 normal subjects indicated that 48% to 56% of the oral dose avoided \"first-pass\" metabolism and was systemically available. Similar estimates were obtained by applying the Loo-Riegelman method for calculating drug absorption viz, 47% to 60%. Oral availability predicted from a knowledge of drug clearance (blood) after intravenouration-time curves after oral administration exhibited irregularities and double peaks possibly indicative of the action of meperidine on the gut or a recycling process.", "contents": "Systemic availability of orally administered meperidine. A comparison of the areas under the plasma concentration-time curves after intravenous and oral administration of meperidine in 4 normal subjects indicated that 48% to 56% of the oral dose avoided \"first-pass\" metabolism and was systemically available. Similar estimates were obtained by applying the Loo-Riegelman method for calculating drug absorption viz, 47% to 60%. Oral availability predicted from a knowledge of drug clearance (blood) after intravenouration-time curves after oral administration exhibited irregularities and double peaks possibly indicative of the action of meperidine on the gut or a recycling process.", "PMID": 975724}
{"id": "pubmed23n0032_10444", "title": "Gastric and biliary excretion of meperidine in man.", "content": "The role and importance of enterogastric secretion in the disposition and elimination of the weak base, meperidine (pKa 8.63), was studied after intravenous administration (50 mg) of the drug to 6 normal volunteers. Continuous collection of the gastric fluid over a 4-hr period demonstrated the establishment of high gastric fluid/plasma concentration ratios for meperidine (mean about 50, range, 10 to 200). However, the total amount of drug recovered, even after correction for incomplete collection, was only a small percentage of the administered dose. Under basal conditions a mean +/- SE of 1.9 +/- 0.3 mg, equivalent to 3.7% of the administered dose, was found in the total gastric aspirate. Stimulation of gastric secretion by subcutaneous injection of betazole (1.5 mg/kg) increased this recovery to 3.6 +/- 0.3 mg (7.2%) primarily due to the increase in gastric volumen output. Aspiration of the gastric fluid in either the basal or stimulated situation had no observable effect upon the plasma concentration/time profile of meperidine whether assessed by the terminal half-life, t 1/2 beta, or the plasma clearance; control values were 3.8 +/- hr and 1,190 +/- 130 ml/min, respectively. In 2 subjects \"bile fluid\" was also collected for 2.5 hr and found to contain less than 0.2% of the administered dose. Enterosystemic recycling is therefore of minor importance in the disposition and elimination of meperidine in man.", "contents": "Gastric and biliary excretion of meperidine in man. The role and importance of enterogastric secretion in the disposition and elimination of the weak base, meperidine (pKa 8.63), was studied after intravenous administration (50 mg) of the drug to 6 normal volunteers. Continuous collection of the gastric fluid over a 4-hr period demonstrated the establishment of high gastric fluid/plasma concentration ratios for meperidine (mean about 50, range, 10 to 200). However, the total amount of drug recovered, even after correction for incomplete collection, was only a small percentage of the administered dose. Under basal conditions a mean +/- SE of 1.9 +/- 0.3 mg, equivalent to 3.7% of the administered dose, was found in the total gastric aspirate. Stimulation of gastric secretion by subcutaneous injection of betazole (1.5 mg/kg) increased this recovery to 3.6 +/- 0.3 mg (7.2%) primarily due to the increase in gastric volumen output. Aspiration of the gastric fluid in either the basal or stimulated situation had no observable effect upon the plasma concentration/time profile of meperidine whether assessed by the terminal half-life, t 1/2 beta, or the plasma clearance; control values were 3.8 +/- hr and 1,190 +/- 130 ml/min, respectively. In 2 subjects \"bile fluid\" was also collected for 2.5 hr and found to contain less than 0.2% of the administered dose. Enterosystemic recycling is therefore of minor importance in the disposition and elimination of meperidine in man.", "PMID": 975725}
{"id": "pubmed23n0032_10445", "title": "Titration of nicotine intake with full-length and half-length cigarettes.", "content": "Titration, the self-regulation of nicotine intake, was studied in 12 smokers by gas chromatograph assays of urinary nicotine levels. Results demonstrated that excretion of urinary nicotine in the proximal condition (half cigarette close to the filter) did not differ significantly from the whole cigarette condition; however, less nicotine was excreted in the distal condition (half cigarette farther from the filter) because of a rod filtration effect. Subjects extracted proportionately more nicotine from the half than from the whole cigarettes; titration was approximately the same in both half-cigarette conditions. On scales of strength and satisfaction, full-length cigarettes were given the highest rating, followed by proximal and then distal cigarettes.", "contents": "Titration of nicotine intake with full-length and half-length cigarettes. Titration, the self-regulation of nicotine intake, was studied in 12 smokers by gas chromatograph assays of urinary nicotine levels. Results demonstrated that excretion of urinary nicotine in the proximal condition (half cigarette close to the filter) did not differ significantly from the whole cigarette condition; however, less nicotine was excreted in the distal condition (half cigarette farther from the filter) because of a rod filtration effect. Subjects extracted proportionately more nicotine from the half than from the whole cigarettes; titration was approximately the same in both half-cigarette conditions. On scales of strength and satisfaction, full-length cigarettes were given the highest rating, followed by proximal and then distal cigarettes.", "PMID": 975726}
{"id": "pubmed23n0032_10446", "title": "Comparison of the hydroxylation of zoxazolamine and benzo[a]pyrene in human placenta: effect of cigarette smoking.", "content": "The in vitro hydroxylation of zoxazolamine was compared with the hydroxylation of benzo[a]pyrene (BP) in full-term placentas from 11 nonsmokers and from 13 women who smoked cigarettes during pregnancy. Cigarette smoking increased the average zoxazolamine and benzo[a]pyrene hydroxylase activities 13- and 39-fold, respectively. A 59-fold range in benzo[a]pyrene hydroxylase activity and a 28-fold range in zoxazolamine hydroxylase activity were found in the placentas of cigarette smokers. A plot of these two enzyme activities showed that zoxazolamine hydroxylase activity was highly correlated, with benzo[a]pyrene hydroxylase activity in the 24 placentas studied (r = 0.98; p less than 0.001). A strong correlation between the above enzymatic activities was also found in 8 placentas which had been stored for 2 yr at -20 degrees C (r = 0.95; p less than 0.001). The results suggest that benzo[a]pyrene and zoxazolamine are metabolized in the human placenta by the same enzyme or by different systems that are under the same regulatory control.", "contents": "Comparison of the hydroxylation of zoxazolamine and benzo[a]pyrene in human placenta: effect of cigarette smoking. The in vitro hydroxylation of zoxazolamine was compared with the hydroxylation of benzo[a]pyrene (BP) in full-term placentas from 11 nonsmokers and from 13 women who smoked cigarettes during pregnancy. Cigarette smoking increased the average zoxazolamine and benzo[a]pyrene hydroxylase activities 13- and 39-fold, respectively. A 59-fold range in benzo[a]pyrene hydroxylase activity and a 28-fold range in zoxazolamine hydroxylase activity were found in the placentas of cigarette smokers. A plot of these two enzyme activities showed that zoxazolamine hydroxylase activity was highly correlated, with benzo[a]pyrene hydroxylase activity in the 24 placentas studied (r = 0.98; p less than 0.001). A strong correlation between the above enzymatic activities was also found in 8 placentas which had been stored for 2 yr at -20 degrees C (r = 0.95; p less than 0.001). The results suggest that benzo[a]pyrene and zoxazolamine are metabolized in the human placenta by the same enzyme or by different systems that are under the same regulatory control.", "PMID": 975727}
{"id": "pubmed23n0032_10447", "title": "Fluoride kinetics after enflurane anesthesia in healthy and anephric patients and in patients with poor renal function.", "content": "Enflurane, a fluorinated methylethyl ether, is metabolized, in part, to inorganic fluoride. Methoxyflurane has similar metabolism, and cases of fluoride ion-induced renal failure have been reported after its use. This prospective study was initiated to determine fluoride ion kinetics after enflurance anesthesia in 16 healthy patients, 18 anephric patients, and 6 patients each having a creatinine clearance of less than 5 ml/min (on dialysis). Serum and urine inorganic fluoride levels were determined. There was no clinical or statistical significance difference among the 3 groups with respect to maximum inorganic fluoride ion concentration or the time to reach it. The fluoride ion values were never above the 50 muM level that has been reported to cause subclinical renal toxicity. The fluoride ion concentration in serum fell rapidly after termination of anesthesia even in the anephric patients. This is presumed to be due to uptake of the ion by bone. Patients with low creatinine clearance also have low fluoride ion clearance. Statistical but not clinical significance was found in the comparison between pre-enflurane and the 24-hr fluoride ion values in the anephric and low creatine clearance patients, but this did not persist after one dialysis.", "contents": "Fluoride kinetics after enflurane anesthesia in healthy and anephric patients and in patients with poor renal function. Enflurane, a fluorinated methylethyl ether, is metabolized, in part, to inorganic fluoride. Methoxyflurane has similar metabolism, and cases of fluoride ion-induced renal failure have been reported after its use. This prospective study was initiated to determine fluoride ion kinetics after enflurance anesthesia in 16 healthy patients, 18 anephric patients, and 6 patients each having a creatinine clearance of less than 5 ml/min (on dialysis). Serum and urine inorganic fluoride levels were determined. There was no clinical or statistical significance difference among the 3 groups with respect to maximum inorganic fluoride ion concentration or the time to reach it. The fluoride ion values were never above the 50 muM level that has been reported to cause subclinical renal toxicity. The fluoride ion concentration in serum fell rapidly after termination of anesthesia even in the anephric patients. This is presumed to be due to uptake of the ion by bone. Patients with low creatinine clearance also have low fluoride ion clearance. Statistical but not clinical significance was found in the comparison between pre-enflurane and the 24-hr fluoride ion values in the anephric and low creatine clearance patients, but this did not persist after one dialysis.", "PMID": 975728}
{"id": "pubmed23n0032_10448", "title": "Plasma and cerebrospinal fluid concentrations of chlordiazepoxide and its metabolites in surgical patients.", "content": "Thirty otherwise healthy patients received a 100-mg oral dose of chlordiazepoxide HCl just prior to surgical procedures using spinal anesthesia. Fourteen of these patients had also received 100 mg on the night before surgery. Simultaneous samples of venous blood and cerebrospinal fluid (CSF) were taken immediately prior to injection of spinal anesthesia and were assayed for concentrations of chlordiazepoxide (CDX) and its major metabolite, desmethylchlordiazepoxide. Plasma concentrations of CDX ranged from 2.32 to 13.34 mug/ml. Simultaneous CSF concentrations were considerably lower, ranging from 0.04 to 0.34 mug/ml. Equilibration of CDX between plasma and the lumbar sampling site appeared to be complete within 2 hr of the most recent dose. After attainment of distribution equilibrium, simultaneous plasma and CSF concentrations of CDX were hightly correlated (r = 0.76), with a mean CSF-plasma concentrations ratio of only 0.043 (range; 0.02 to 0.06). The limited passage of CDX into human CSF is probably due to extensive binding to plasma protein. Assuming that transfer of CDX from plasma to CSF is governed by passive diffusion, the extent of plasma protein binding of CDX in healthy individuals averages about 96%.", "contents": "Plasma and cerebrospinal fluid concentrations of chlordiazepoxide and its metabolites in surgical patients. Thirty otherwise healthy patients received a 100-mg oral dose of chlordiazepoxide HCl just prior to surgical procedures using spinal anesthesia. Fourteen of these patients had also received 100 mg on the night before surgery. Simultaneous samples of venous blood and cerebrospinal fluid (CSF) were taken immediately prior to injection of spinal anesthesia and were assayed for concentrations of chlordiazepoxide (CDX) and its major metabolite, desmethylchlordiazepoxide. Plasma concentrations of CDX ranged from 2.32 to 13.34 mug/ml. Simultaneous CSF concentrations were considerably lower, ranging from 0.04 to 0.34 mug/ml. Equilibration of CDX between plasma and the lumbar sampling site appeared to be complete within 2 hr of the most recent dose. After attainment of distribution equilibrium, simultaneous plasma and CSF concentrations of CDX were hightly correlated (r = 0.76), with a mean CSF-plasma concentrations ratio of only 0.043 (range; 0.02 to 0.06). The limited passage of CDX into human CSF is probably due to extensive binding to plasma protein. Assuming that transfer of CDX from plasma to CSF is governed by passive diffusion, the extent of plasma protein binding of CDX in healthy individuals averages about 96%.", "PMID": 975729}
{"id": "pubmed23n0032_10449", "title": "Observations in man on some pharmacologic features of cefamandole.", "content": "Comparative studies of cefamandole and cephalothin were carried out in 32 cancer patients. After rapid intravenous injection of 1 gm cefamandole or cephalothin, the peak mean serum concentrations in 11 patients achieved at 0.25 hr were 103.4 mcg/ml and 56.7 mcg/ml, respectively. Except at 6 hr, the serum concentration of cefamandole was higher (p less than 0.05) at all times. The terminal half-lives (t 1/2) were similar, being 1.2 hr for cefamandole and 1.0 hr for cephalothin. Cefamandole, 1 gm intramuscularly, induced a peak mean serum concentration of 26.6 mcg/ml at 1 hr, with a slow decay. Intermittent cefamandole (2 gm intravenously every 6 hr) induced very high mean serum concentrations (7 patients), but at 4 hr the concentrations were similar to those after 1 gm intravenously. Per cent of urinary excretion was similar for both drugs regardless of dose and mode of administration. Continuous-infusion cefamandole or cephalothin (2 gm loading followed by 2 gm every 6 hr) in 14 patients showed consistently higher serum concentrations for cefamandole (p less than 0.05) over a 5-day period. There was no evidence of drug accumulation in the multiple-dose studies. Both the single- and multiple-dose schedules were well tolerated.", "contents": "Observations in man on some pharmacologic features of cefamandole. Comparative studies of cefamandole and cephalothin were carried out in 32 cancer patients. After rapid intravenous injection of 1 gm cefamandole or cephalothin, the peak mean serum concentrations in 11 patients achieved at 0.25 hr were 103.4 mcg/ml and 56.7 mcg/ml, respectively. Except at 6 hr, the serum concentration of cefamandole was higher (p less than 0.05) at all times. The terminal half-lives (t 1/2) were similar, being 1.2 hr for cefamandole and 1.0 hr for cephalothin. Cefamandole, 1 gm intramuscularly, induced a peak mean serum concentration of 26.6 mcg/ml at 1 hr, with a slow decay. Intermittent cefamandole (2 gm intravenously every 6 hr) induced very high mean serum concentrations (7 patients), but at 4 hr the concentrations were similar to those after 1 gm intravenously. Per cent of urinary excretion was similar for both drugs regardless of dose and mode of administration. Continuous-infusion cefamandole or cephalothin (2 gm loading followed by 2 gm every 6 hr) in 14 patients showed consistently higher serum concentrations for cefamandole (p less than 0.05) over a 5-day period. There was no evidence of drug accumulation in the multiple-dose studies. Both the single- and multiple-dose schedules were well tolerated.", "PMID": 975730}
{"id": "pubmed23n0032_10450", "title": "Hemodialysis for severe procainamide toxicity: clinical and pharmacokinetic observations.", "content": "A 67-yr-old woman who ingested approximately 7 gm procainamide developed severe hypotension, renal insufficiency, and life-threatening cardiac toxicity. Hemodialysis doubled the rate of procainamide elimination and increased fourfold the clearance of NAPA, the N-acetylated metabolite of procainamide. Observations of procainamide and N-acetylprocainamide (NAPA) plasma levels during the patient's recovery suggest that lethargy and profound hypotension can be expected when these levels total 60 mug/ml and that severe cardiac toxicity should be anticipated with levels totaling 42 mug/ml or more. Hemodialysis also permitted investigation of the effects of hypotension on the pharmacokinetics of these compounds. The apparent volume of procainamide distribution was reduced from a normal value of 2 L/kg to 0.76 L/kg, and that of NAPA from 1.4 L/kg to 0.63 L/kg. The elimination + 1/2 of procainamide was prolonged from the normal of 3 hr to 10.5 hr, and that of NAPA from 6 to 35.9 hr. Procainamide absorption was also slowed in this clinical setting, causing procainamide plasma levels to continue rising for some time after toxicity was first recognized.", "contents": "Hemodialysis for severe procainamide toxicity: clinical and pharmacokinetic observations. A 67-yr-old woman who ingested approximately 7 gm procainamide developed severe hypotension, renal insufficiency, and life-threatening cardiac toxicity. Hemodialysis doubled the rate of procainamide elimination and increased fourfold the clearance of NAPA, the N-acetylated metabolite of procainamide. Observations of procainamide and N-acetylprocainamide (NAPA) plasma levels during the patient's recovery suggest that lethargy and profound hypotension can be expected when these levels total 60 mug/ml and that severe cardiac toxicity should be anticipated with levels totaling 42 mug/ml or more. Hemodialysis also permitted investigation of the effects of hypotension on the pharmacokinetics of these compounds. The apparent volume of procainamide distribution was reduced from a normal value of 2 L/kg to 0.76 L/kg, and that of NAPA from 1.4 L/kg to 0.63 L/kg. The elimination + 1/2 of procainamide was prolonged from the normal of 3 hr to 10.5 hr, and that of NAPA from 6 to 35.9 hr. Procainamide absorption was also slowed in this clinical setting, causing procainamide plasma levels to continue rising for some time after toxicity was first recognized.", "PMID": 975731}
{"id": "pubmed23n0032_10451", "title": "The radiology of thoracic disc protrusion.", "content": "The radiological features of 20 surgically proven thoracic disc protrusions are reviewed. In 15 (75%), characteristic plain film changes were found to be very helpful in making the correct diagnosis. Calcified disc material in the spinal canal was clearly demonstrated in 11 (55%) cases, and this, in association with linear calcification in the adjacent disc space, has been shown to be diagnostic of the condition. At myelography, central protrusions (55%) were found to be predominant, while lateral protrusions were uncommon. The differential diagnosis of a calcified lesion in the spinal canal includes thoracic disc protrusion, osteochondroma and meningioma.", "contents": "The radiology of thoracic disc protrusion. The radiological features of 20 surgically proven thoracic disc protrusions are reviewed. In 15 (75%), characteristic plain film changes were found to be very helpful in making the correct diagnosis. Calcified disc material in the spinal canal was clearly demonstrated in 11 (55%) cases, and this, in association with linear calcification in the adjacent disc space, has been shown to be diagnostic of the condition. At myelography, central protrusions (55%) were found to be predominant, while lateral protrusions were uncommon. The differential diagnosis of a calcified lesion in the spinal canal includes thoracic disc protrusion, osteochondroma and meningioma.", "PMID": 975736}
{"id": "pubmed23n0032_10452", "title": "Complications of percutaneous transfemoral coronary arteriography.", "content": "Complications statistics in 1625 patients undergoing selective coronary angiography by Judkin's technique have been analysed for four major areas-death, myocardial infarction, cerebral vascular accidents, and femoral thrombosis. Over a six-year period, considerable decrease in the number of complications has occurred. Myocardial infarctions have decreased from 2.4% (1967-71) to 0.44% (1973), an improvement related to careful catheter flushing, guidewire insertion technique and careful pressure monitoring within the coronary arteries. Cerebral accidents have decreased from a maximum of 1.3% (1972) to none (1973). This is also related to catheter guidewire techniques, and to avoidance of entering the cerebral vessels inadvertently when crossing the aortic arch with the coronary catheter. Femoral artery thromboses have decreased from 2.7% (1967-71) to none (1973). This is due to monitoring the dorsalis pedis pulse when compressing the femoral artery and avoiding complete femoral arterial obstruction with the associated lack of flow ultimately leading to thrombosis. Death rate has remained essentially unchanged (0.3-0.6%).", "contents": "Complications of percutaneous transfemoral coronary arteriography. Complications statistics in 1625 patients undergoing selective coronary angiography by Judkin's technique have been analysed for four major areas-death, myocardial infarction, cerebral vascular accidents, and femoral thrombosis. Over a six-year period, considerable decrease in the number of complications has occurred. Myocardial infarctions have decreased from 2.4% (1967-71) to 0.44% (1973), an improvement related to careful catheter flushing, guidewire insertion technique and careful pressure monitoring within the coronary arteries. Cerebral accidents have decreased from a maximum of 1.3% (1972) to none (1973). This is also related to catheter guidewire techniques, and to avoidance of entering the cerebral vessels inadvertently when crossing the aortic arch with the coronary catheter. Femoral artery thromboses have decreased from 2.7% (1967-71) to none (1973). This is due to monitoring the dorsalis pedis pulse when compressing the femoral artery and avoiding complete femoral arterial obstruction with the associated lack of flow ultimately leading to thrombosis. Death rate has remained essentially unchanged (0.3-0.6%).", "PMID": 975738}
{"id": "pubmed23n0032_10453", "title": "Oliguria in the incubating neonate. Emergency aortography and urography in neonates using mobile X-ray equipment.", "content": "Neonates in incubators underwent umbilical aortography, excretory urography and suprapubic cysto-urethrography using a mobile X-ray machine in the Premature Baby Unit to define the cause of their oliguria. The techniques were easy to perform without endangering the infants by moving them. The radiological findings in the four neonates investigated contributed significantly to the management of their illnesses.", "contents": "Oliguria in the incubating neonate. Emergency aortography and urography in neonates using mobile X-ray equipment. Neonates in incubators underwent umbilical aortography, excretory urography and suprapubic cysto-urethrography using a mobile X-ray machine in the Premature Baby Unit to define the cause of their oliguria. The techniques were easy to perform without endangering the infants by moving them. The radiological findings in the four neonates investigated contributed significantly to the management of their illnesses.", "PMID": 975739}
{"id": "pubmed23n0032_10454", "title": "Progeria in siblings.", "content": "Progeria or 'prematurely old' is an autosomal recessive trait that appears insidiously at or after the age of 6 months. Two affected siblings are reported showing radiologically progressive attenuation of the clavicles and terminal phalanges, widening of cranial sutures and mandibular hypoplasia. Scleroderma was also present. Out of 59 previously published cases five had scleroderma before the age of 2 years. Only three families with affected siblings have been previously recorded.", "contents": "Progeria in siblings. Progeria or 'prematurely old' is an autosomal recessive trait that appears insidiously at or after the age of 6 months. Two affected siblings are reported showing radiologically progressive attenuation of the clavicles and terminal phalanges, widening of cranial sutures and mandibular hypoplasia. Scleroderma was also present. Out of 59 previously published cases five had scleroderma before the age of 2 years. Only three families with affected siblings have been previously recorded.", "PMID": 975740}
{"id": "pubmed23n0032_10455", "title": "Articular mass fracture: a neglected cause of post-traumatic neck pain?", "content": "Fifty-one compressed distorted cervical articular masses were found in 29 patients seen at this institution over a nine-month period. The significance of these findings is discussed and it is concluded that in most instances these represented compression fractures with deformity of the articular process. Selected signs suggestive of a compressed articular mass are identified on various views. These include (1) neuroforaminal encroachment or narrowing, (2) triangular appearance of the lateral mass in the oblique view, (3) unilateral anterior displacement of the superior facet with respect to the inferior facet, (4) minor anterior subluxation of one vertebral body over another, (5) double outline sign on the lateral view, (6) abnormally visible posterior articulations on the routine AP view. The articular processes are best shown on anteroposterior angled caudad views and lateral tomography. Articular mass deformity frequently appears to be associated with persistent post-traumatic neck pain and a relationship between this deformity and primary or secondary degenerative cervical disc and joint disease is suggested.", "contents": "Articular mass fracture: a neglected cause of post-traumatic neck pain? Fifty-one compressed distorted cervical articular masses were found in 29 patients seen at this institution over a nine-month period. The significance of these findings is discussed and it is concluded that in most instances these represented compression fractures with deformity of the articular process. Selected signs suggestive of a compressed articular mass are identified on various views. These include (1) neuroforaminal encroachment or narrowing, (2) triangular appearance of the lateral mass in the oblique view, (3) unilateral anterior displacement of the superior facet with respect to the inferior facet, (4) minor anterior subluxation of one vertebral body over another, (5) double outline sign on the lateral view, (6) abnormally visible posterior articulations on the routine AP view. The articular processes are best shown on anteroposterior angled caudad views and lateral tomography. Articular mass deformity frequently appears to be associated with persistent post-traumatic neck pain and a relationship between this deformity and primary or secondary degenerative cervical disc and joint disease is suggested.", "PMID": 975741}
{"id": "pubmed23n0032_10456", "title": "The 'tilt deformity' of the femoral head in osteoarthritis of the hip: a poor indicator of previous epiphysiolysis.", "content": "Apparent superomedial migration of the femoral head producing the 'tilt deformity' is the most frequent pattern of femoral head deformity in osteoarthritis of the hip. Previous reports have suggested a relationship to minor degrees of epiphysiolysis in the adolescent. Radiological and pathological examination of femoral heads obtained at the time of total hip replacement for osteoarthritis demonstrate characteristic abnormalities accompanying the 'tilt deformity'. It would appear that, in most patients, this pattern of femoral head migration results not from epiphysiolysis but from a remodelling process in the osteoarthritic joint.", "contents": "The 'tilt deformity' of the femoral head in osteoarthritis of the hip: a poor indicator of previous epiphysiolysis. Apparent superomedial migration of the femoral head producing the 'tilt deformity' is the most frequent pattern of femoral head deformity in osteoarthritis of the hip. Previous reports have suggested a relationship to minor degrees of epiphysiolysis in the adolescent. Radiological and pathological examination of femoral heads obtained at the time of total hip replacement for osteoarthritis demonstrate characteristic abnormalities accompanying the 'tilt deformity'. It would appear that, in most patients, this pattern of femoral head migration results not from epiphysiolysis but from a remodelling process in the osteoarthritic joint.", "PMID": 975742}
{"id": "pubmed23n0032_10457", "title": "Peripheral arteriographic studies in leprosy.", "content": "There is a considerable controversy regarding the frequency and significance of vascular lesions in leprosy. Twenty-six cases of leprosy below the age of 40 years, without any local or systemic arterial disease and normal looking hands were subjected to brachial arteriography. One patient had posterior tibial arteriography also. The diagnosis of leprosy was confirmed by histology or bacteriological staining techniques. Biopsy material was studied from the involved areas and similar material was obtained from nine matched control subjects at autopsy. Skin thermometry and reflex vasodilatation was studied in eight cases. Arteriographic abnormalities such as occlusion, narrowing, tortuosity, dilatation, irregularity and incomplete filling were seen in more than two vessels in the hand in 50%, and in the digits in 75% of the cases. The ulnar artery was more frequently involved than the radial. Superficial and deep palmar arches were equally affected. Marked increase in arcuate vessels was noticed in nearly three-quarters of the patients, and in some, arcuate vessles were serving as collaterals. Histologically all sizes of vessles, including the capillaries, showed changes in nearly half the cases. Reflex vasodilation was lost in three cases of dimorphous leprosy and impaired or absent in three out of five cases of lepromatous leprosy. There was no predilection for any form of leprosy to show arterial changes more than the other. This study clearly demonstrates that the arterial involvement in leprosy is very frequent and must be playing an important role in causing mutilation and deformities of hands and feet.", "contents": "Peripheral arteriographic studies in leprosy. There is a considerable controversy regarding the frequency and significance of vascular lesions in leprosy. Twenty-six cases of leprosy below the age of 40 years, without any local or systemic arterial disease and normal looking hands were subjected to brachial arteriography. One patient had posterior tibial arteriography also. The diagnosis of leprosy was confirmed by histology or bacteriological staining techniques. Biopsy material was studied from the involved areas and similar material was obtained from nine matched control subjects at autopsy. Skin thermometry and reflex vasodilatation was studied in eight cases. Arteriographic abnormalities such as occlusion, narrowing, tortuosity, dilatation, irregularity and incomplete filling were seen in more than two vessels in the hand in 50%, and in the digits in 75% of the cases. The ulnar artery was more frequently involved than the radial. Superficial and deep palmar arches were equally affected. Marked increase in arcuate vessels was noticed in nearly three-quarters of the patients, and in some, arcuate vessles were serving as collaterals. Histologically all sizes of vessles, including the capillaries, showed changes in nearly half the cases. Reflex vasodilation was lost in three cases of dimorphous leprosy and impaired or absent in three out of five cases of lepromatous leprosy. There was no predilection for any form of leprosy to show arterial changes more than the other. This study clearly demonstrates that the arterial involvement in leprosy is very frequent and must be playing an important role in causing mutilation and deformities of hands and feet.", "PMID": 975743}
{"id": "pubmed23n0032_10458", "title": "Mammograms on medichrome film.", "content": "When medichrome film is vacuum packed and used in combination with a single high definition intensifying screen exposure times of 0.06-0.1 s and a skin radiation dosage 0.5 rad per exposure can be achieved. The diagnostic accuracy of the system is similar to that obtained on conventional black and white film currently used for mammography.", "contents": "Mammograms on medichrome film. When medichrome film is vacuum packed and used in combination with a single high definition intensifying screen exposure times of 0.06-0.1 s and a skin radiation dosage 0.5 rad per exposure can be achieved. The diagnostic accuracy of the system is similar to that obtained on conventional black and white film currently used for mammography.", "PMID": 975744}
{"id": "pubmed23n0032_10459", "title": "The role of the physicist in a diagnostic radiology department.", "content": "A physicist working full-time within a department of diagnostic radiology has found himself concerned with the development of investigative techniques using non-X-ray apparatus, such as thermography, as well as with testing and assessing new X-ray techniques, such as xerography. The scientific training of the physicist enables him to advise and instruct on other research projects. His links with physicists elsewhere are advantageous because of the larger range of specific expertise and available technology. It is thought that the arrangement is successful, should continue, and be tried elsewhere.", "contents": "The role of the physicist in a diagnostic radiology department. A physicist working full-time within a department of diagnostic radiology has found himself concerned with the development of investigative techniques using non-X-ray apparatus, such as thermography, as well as with testing and assessing new X-ray techniques, such as xerography. The scientific training of the physicist enables him to advise and instruct on other research projects. His links with physicists elsewhere are advantageous because of the larger range of specific expertise and available technology. It is thought that the arrangement is successful, should continue, and be tried elsewhere.", "PMID": 975745}
{"id": "pubmed23n0032_10460", "title": "A system of organisation of an X-ray film library.", "content": "The physical arrangement of radiographs and a method for their retrieval is discussed. The aim is to facilitate access to examples of various conditions from several possible approaches by a method of specific classification of each item and a comprehensive card index. A system to maintain consistency and to achieve relevance for the potential user is described.", "contents": "A system of organisation of an X-ray film library. The physical arrangement of radiographs and a method for their retrieval is discussed. The aim is to facilitate access to examples of various conditions from several possible approaches by a method of specific classification of each item and a comprehensive card index. A system to maintain consistency and to achieve relevance for the potential user is described.", "PMID": 975746}
{"id": "pubmed23n0032_10461", "title": "Thermography in lower limb arterial disease.", "content": "Skin temperature distributions are described which correspond to different levels of arterial obstruction in the lower limbs. These temperature distributions are conveniently observed using thermography. Thermography may be used to measure the change in skin temperature when the limb performs work. These skin temperature changes have been correlated with the impairment of blood flow to the lower limb.", "contents": "Thermography in lower limb arterial disease. Skin temperature distributions are described which correspond to different levels of arterial obstruction in the lower limbs. These temperature distributions are conveniently observed using thermography. Thermography may be used to measure the change in skin temperature when the limb performs work. These skin temperature changes have been correlated with the impairment of blood flow to the lower limb.", "PMID": 975747}
{"id": "pubmed23n0032_10462", "title": "Review of parameters characterising response of normal connective tissue to radiation.", "content": "A comparison is made of the formalisms which have been proposed to characterise the response of normal connective tissue to levels of radiation which may lead to end points other than tissue tolerance; namely, Partial Tolerance, Time-Dose-Fractionation factor, and the Cumulative Radiation Effect. It is shown that, in most situations, conclusions which may be drawn using any one formalism are essentially the same as those which follow from the use of any other.", "contents": "Review of parameters characterising response of normal connective tissue to radiation. A comparison is made of the formalisms which have been proposed to characterise the response of normal connective tissue to levels of radiation which may lead to end points other than tissue tolerance; namely, Partial Tolerance, Time-Dose-Fractionation factor, and the Cumulative Radiation Effect. It is shown that, in most situations, conclusions which may be drawn using any one formalism are essentially the same as those which follow from the use of any other.", "PMID": 975748}
{"id": "pubmed23n0032_10463", "title": "Automated analysis of phencyclidine in urine by probability based matching GC/MS.", "content": "A novel form of selected ion recording mass spectrometry using a microcomputer-managed mass spectrometer was employed to automatically identify and quantitate phencyclidine (PCP) in cyclohexane extracts of urine by Probability Based Matching. Seventy urine samples from known abusers were assayed for PCP content. The positively identified PCP concentrations ranged from 0.01 to 10.5 mug/ml for 65 samples, 26% of which fell in the 0.35-1.0 mug/ml range and 30% in the 1.0-3.4 mug/ml range. Five specimens had no detectable PCP (less than 10 mug/ml). Cyclohexane extraction efficiency for PCP in urine exceeded 95%. Selected ion monitoring was found to be necessary in order to avoid gas chromatographic interferences produced by co-elution of contaminants at the same retention time as PCP.", "contents": "Automated analysis of phencyclidine in urine by probability based matching GC/MS. A novel form of selected ion recording mass spectrometry using a microcomputer-managed mass spectrometer was employed to automatically identify and quantitate phencyclidine (PCP) in cyclohexane extracts of urine by Probability Based Matching. Seventy urine samples from known abusers were assayed for PCP content. The positively identified PCP concentrations ranged from 0.01 to 10.5 mug/ml for 65 samples, 26% of which fell in the 0.35-1.0 mug/ml range and 30% in the 1.0-3.4 mug/ml range. Five specimens had no detectable PCP (less than 10 mug/ml). Cyclohexane extraction efficiency for PCP in urine exceeded 95%. Selected ion monitoring was found to be necessary in order to avoid gas chromatographic interferences produced by co-elution of contaminants at the same retention time as PCP.", "PMID": 975752}
{"id": "pubmed23n0032_10464", "title": "Phencyclidine: tissue distribution in the rat.", "content": "This study was performed to provide knowledge of the tissue distribution of phencyclidine and has demonstrated the lipophilic nature of the drug. The distribution of phencyclidine in blood, brain, and adipose tissue of rats has been determined at various time intervals during a 48-hr period. The affinity of phencyclidine for adipose tissue and the demonstration of the presence of this drug in brain tissue long after it is no longer detectable in blood provides some correlation between the tissue distribution of phencyclidine and its clinical manifestations occuring 24-48 hr after administration.", "contents": "Phencyclidine: tissue distribution in the rat. This study was performed to provide knowledge of the tissue distribution of phencyclidine and has demonstrated the lipophilic nature of the drug. The distribution of phencyclidine in blood, brain, and adipose tissue of rats has been determined at various time intervals during a 48-hr period. The affinity of phencyclidine for adipose tissue and the demonstration of the presence of this drug in brain tissue long after it is no longer detectable in blood provides some correlation between the tissue distribution of phencyclidine and its clinical manifestations occuring 24-48 hr after administration.", "PMID": 975753}
{"id": "pubmed23n0032_10465", "title": "Metabolites of phencyclidine.", "content": "The results of the animal experiment demonstrate that oxidative hydroxylation is the major mode of metabolism of phencyclidine. It is evident that this process takes place in all three rings of the molecule. Hydroxylation of the piperidyl moiety probably also accounts for the formation of the N-dealkylated metabolites. Metabolism of phencyclidine in humans appears, at least in part, to be similar to that in the rat. Hydroxylation is still the principal mode in the case of humans though of a lesser extent. Metabolites hydroxylated in the phenyl moiety as well as dihydroxy derivatives of phencyclidine have so far not been observed in humans. Furthermore, most of the monohydroxy metabolites exist as conjugates in the human urine. It should also be pointed out that no metabolites have yet been detected in human blood samples.", "contents": "Metabolites of phencyclidine. The results of the animal experiment demonstrate that oxidative hydroxylation is the major mode of metabolism of phencyclidine. It is evident that this process takes place in all three rings of the molecule. Hydroxylation of the piperidyl moiety probably also accounts for the formation of the N-dealkylated metabolites. Metabolism of phencyclidine in humans appears, at least in part, to be similar to that in the rat. Hydroxylation is still the principal mode in the case of humans though of a lesser extent. Metabolites hydroxylated in the phenyl moiety as well as dihydroxy derivatives of phencyclidine have so far not been observed in humans. Furthermore, most of the monohydroxy metabolites exist as conjugates in the human urine. It should also be pointed out that no metabolites have yet been detected in human blood samples.", "PMID": 975754}
{"id": "pubmed23n0032_10466", "title": "Proteolytic enzymes in human cartilage: the pathogenesis of osteoarthritis.", "content": "It is evident that human articular cartilage possesses, in addition to multiple forms of cathepsin D, multiple forms of other acid cathepsins, and, most important, a family of at least four closely related neutral protease enzyme forms, all of which degrade proteoglycan. In addition, caseinase and histonase activities are present. The search for these enzymes in human cartilage ahs been presented in some detail in order to give an idea of some of the problems faced in such research, as well as the hypotheses and hopes that flow from it and prepare the ground for further research. The actual role of proteolytic enzymes in the physiologic and pathologic condition of cartilage remains to be determined. It is hoped that these enzymes, especially the neutral protease forms, will be sufficiently purified to enable preparation of antibodies to them. This will help to clarify what controls their release from the chondrocytes and where they function in the cartilage. Meanwhile, it seems appropriate to study the neutral protease forms and their role in initiating the degradation of proteoglycan in the early stages of osteoarthritis. The chief role of cathepsin D and the new acid cathepsins is most likely in intracellular digestion. One may hypothesize a three-step sequence of the degradation of the matrix proteoglycan: (1) initial extracellular attack by the neutral matrix, (2) endocytosis of the fragments by the cells, and (3) completion of their degradation within the lysosomal digestive system of the cell. The initial degradation of the matrix proteoglycan would facilitate the entrance of other degrading enzymes from the synovium to aid in total destruction of the cartilage. While awaiting knowledge of the primary events that trigger the development of osteoarthritis, enzymatic research offers the real hope of finding a way to control the enzymatic degradation of proteoglycan occurring in the early stages of the disease. Research into the nature of these degrading enzymes will lead to the development of therapeutically suitable inhibitors.", "contents": "Proteolytic enzymes in human cartilage: the pathogenesis of osteoarthritis. It is evident that human articular cartilage possesses, in addition to multiple forms of cathepsin D, multiple forms of other acid cathepsins, and, most important, a family of at least four closely related neutral protease enzyme forms, all of which degrade proteoglycan. In addition, caseinase and histonase activities are present. The search for these enzymes in human cartilage ahs been presented in some detail in order to give an idea of some of the problems faced in such research, as well as the hypotheses and hopes that flow from it and prepare the ground for further research. The actual role of proteolytic enzymes in the physiologic and pathologic condition of cartilage remains to be determined. It is hoped that these enzymes, especially the neutral protease forms, will be sufficiently purified to enable preparation of antibodies to them. This will help to clarify what controls their release from the chondrocytes and where they function in the cartilage. Meanwhile, it seems appropriate to study the neutral protease forms and their role in initiating the degradation of proteoglycan in the early stages of osteoarthritis. The chief role of cathepsin D and the new acid cathepsins is most likely in intracellular digestion. One may hypothesize a three-step sequence of the degradation of the matrix proteoglycan: (1) initial extracellular attack by the neutral matrix, (2) endocytosis of the fragments by the cells, and (3) completion of their degradation within the lysosomal digestive system of the cell. The initial degradation of the matrix proteoglycan would facilitate the entrance of other degrading enzymes from the synovium to aid in total destruction of the cartilage. While awaiting knowledge of the primary events that trigger the development of osteoarthritis, enzymatic research offers the real hope of finding a way to control the enzymatic degradation of proteoglycan occurring in the early stages of the disease. Research into the nature of these degrading enzymes will lead to the development of therapeutically suitable inhibitors.", "PMID": 975757}
{"id": "pubmed23n0032_10467", "title": "The single hot joint.", "content": "Several points may be stressed.  (1)When in doubt, perform joint aspiration and look for crystals of micro-organisms. A joint tap is nearly always indicated.  (2)Do not rely on a coincidental elevated serum uric acid level.  Question the patient regarding drug therapy and other causes of secondary hyperuricemia.  (3)Examine all of the patient, looking for tophi (gout), skin lesions (gonococcal infection, psoriasis), erythema nodosum (allergic reactions, fungal infections), and other clues.  (4)Monoarticular rheumatoid arthritis is a rare cause of a single hot joint, but it is much more common that the real rarities (e.g., pigmented willondular synovitis).  (5)Anky-losing spondylitis and Reiter's syndrome are common, yet frequently overlooked.  (6)Radiologic examination is usually not helpful.  (7)Having ruled out infection, crystal synovitis, and hemorrhage, it is sufficient to introduce symptomatic treatment and await the natural development of the joint disease.  Follow-up in four to six weeks and simple blood studies often reveal the definitive diagnosis.  Most of the time, natural healing processes are effective , and reward the patience of the conservative physician.  Blind management must always be avoided.", "contents": "The single hot joint. Several points may be stressed.  (1)When in doubt, perform joint aspiration and look for crystals of micro-organisms. A joint tap is nearly always indicated.  (2)Do not rely on a coincidental elevated serum uric acid level.  Question the patient regarding drug therapy and other causes of secondary hyperuricemia.  (3)Examine all of the patient, looking for tophi (gout), skin lesions (gonococcal infection, psoriasis), erythema nodosum (allergic reactions, fungal infections), and other clues.  (4)Monoarticular rheumatoid arthritis is a rare cause of a single hot joint, but it is much more common that the real rarities (e.g., pigmented willondular synovitis).  (5)Anky-losing spondylitis and Reiter's syndrome are common, yet frequently overlooked.  (6)Radiologic examination is usually not helpful.  (7)Having ruled out infection, crystal synovitis, and hemorrhage, it is sufficient to introduce symptomatic treatment and await the natural development of the joint disease.  Follow-up in four to six weeks and simple blood studies often reveal the definitive diagnosis.  Most of the time, natural healing processes are effective , and reward the patience of the conservative physician.  Blind management must always be avoided.", "PMID": 975758}
{"id": "pubmed23n0032_10468", "title": "Medical management of rheumatoid arthritis.", "content": "The overall management of RA is multifaceted. It includes rest, both systemic and articular; physical therapy; utilization of the techniques and appliances of occupational therapy; drugs, both those that suppress inflammation and those that are capable of altering the disease course itself; a knowledge of specific articular and nonarticular complications; and the ability to refer for appropriate surgical management. Judicious, energetic application of these principles can favorably affect the outcome of rheumatoid disease in most patients.", "contents": "Medical management of rheumatoid arthritis. The overall management of RA is multifaceted. It includes rest, both systemic and articular; physical therapy; utilization of the techniques and appliances of occupational therapy; drugs, both those that suppress inflammation and those that are capable of altering the disease course itself; a knowledge of specific articular and nonarticular complications; and the ability to refer for appropriate surgical management. Judicious, energetic application of these principles can favorably affect the outcome of rheumatoid disease in most patients.", "PMID": 975759}
{"id": "pubmed23n0032_10469", "title": "Infection in rheumatoid arthritis.", "content": "Sepsis is an unusually common cause of illness and death in RA. All sorts of infections occur, but pyarthrosis produces exceptional problems. Clinically, pyarthrosis, empyema, and purulent pericarditis mimic bland rheumatoid effusions. Aspiration of the attendant effusions is the only reliable diagnostic procedure. Subcutaneous nodules on the sacrum and back are easily overlooked. Necrosis and ulceration of these nodules may provoke septicemia. Those with Felty's syndrome do not uniformly have problems with recurrent infection. Splenectomy may not benefit such patients. The belief that corticosteroids cause increased infections in rheumatoid patients is not totally justifiable at present. Steroids can, however, disguise underlying sepsis and hamper proper diagnosis.", "contents": "Infection in rheumatoid arthritis. Sepsis is an unusually common cause of illness and death in RA. All sorts of infections occur, but pyarthrosis produces exceptional problems. Clinically, pyarthrosis, empyema, and purulent pericarditis mimic bland rheumatoid effusions. Aspiration of the attendant effusions is the only reliable diagnostic procedure. Subcutaneous nodules on the sacrum and back are easily overlooked. Necrosis and ulceration of these nodules may provoke septicemia. Those with Felty's syndrome do not uniformly have problems with recurrent infection. Splenectomy may not benefit such patients. The belief that corticosteroids cause increased infections in rheumatoid patients is not totally justifiable at present. Steroids can, however, disguise underlying sepsis and hamper proper diagnosis.", "PMID": 975760}
{"id": "pubmed23n0032_10470", "title": "Treatment of asymptomatic diabetes mellitus.", "content": "A large number of individuals currently diagnosed as having diabetes mellitus are asymptomatic. In order to provide rational therapy for this patient population, it is necessary to focus upon the differences between these patients and the classic prototypes with polyuria and weight loss, who require insulin for survival. Patients with asymptomatic diabetes do not need insulin for survival, and, by definition, they do not need it to alleviate symptoms. They tend to be middle-aged and overweight, but they can be young and thin. Their degree of hyperglycemia is moderate, often indistinguishable from that of normal individuals in their day-to-day existence. Indeed, they can often be differentiated from normal persons only on the basis of their blood glucose response to the stress of a large dextrose challenge; in this regard, the potential problem of over-diagnosing diabetes has been discussed. Since the major problem facing patients with asymptomatic diabetes is accelerated atherogenesis, the therapeutic approach must be based upon efforts to delay or prevent the onset of vascular disease. It has yet to be shown that any therapeutic intervention helps such patients, but an argument has been made in support of the following goals in subjects with asymptomatic diabetes whose fasting blood glucose level is less than 170 mg/100 ml: (1) stop smoking, (2) control hypertension, (3) attain ideal body weight, and (4) maintain blood triglyceride and cholesterol levels well within normal limits. Attempts to lower blood glucose with either insulin or oral agents do not seem indicated in the majority of patients within this defined diabetic population.", "contents": "Treatment of asymptomatic diabetes mellitus. A large number of individuals currently diagnosed as having diabetes mellitus are asymptomatic. In order to provide rational therapy for this patient population, it is necessary to focus upon the differences between these patients and the classic prototypes with polyuria and weight loss, who require insulin for survival. Patients with asymptomatic diabetes do not need insulin for survival, and, by definition, they do not need it to alleviate symptoms. They tend to be middle-aged and overweight, but they can be young and thin. Their degree of hyperglycemia is moderate, often indistinguishable from that of normal individuals in their day-to-day existence. Indeed, they can often be differentiated from normal persons only on the basis of their blood glucose response to the stress of a large dextrose challenge; in this regard, the potential problem of over-diagnosing diabetes has been discussed. Since the major problem facing patients with asymptomatic diabetes is accelerated atherogenesis, the therapeutic approach must be based upon efforts to delay or prevent the onset of vascular disease. It has yet to be shown that any therapeutic intervention helps such patients, but an argument has been made in support of the following goals in subjects with asymptomatic diabetes whose fasting blood glucose level is less than 170 mg/100 ml: (1) stop smoking, (2) control hypertension, (3) attain ideal body weight, and (4) maintain blood triglyceride and cholesterol levels well within normal limits. Attempts to lower blood glucose with either insulin or oral agents do not seem indicated in the majority of patients within this defined diabetic population.", "PMID": 975761}
{"id": "pubmed23n0032_10471", "title": "The management of diabetes in the elderly.", "content": "It appears inevitable that with increased longevity, the management of the elderly diabetic will place even greater demands on hospital services. It seems reasonable to adopt a more liberal attitude to the regulation of control of diabetes in the elderly than in younger patients. However, the view that diabetes in the elderly is always mild can be dangerously misleading. A significant number of elderly diabetics develop ketoacidosis or other serious forms of metabolic disturbance, and in these patients the mortality is high. Cardiovascular disease and peripheral vascular disease are major problems in the elderly, the former being the main cause of death. For best results, it is desirable that management of diabetes not be isolated from management of other, coexisting disorders, but rather that it be considered as part of the overall patient problem. As Malins pointed out, we should think in terms of diabetics rather than diabetes.", "contents": "The management of diabetes in the elderly. It appears inevitable that with increased longevity, the management of the elderly diabetic will place even greater demands on hospital services. It seems reasonable to adopt a more liberal attitude to the regulation of control of diabetes in the elderly than in younger patients. However, the view that diabetes in the elderly is always mild can be dangerously misleading. A significant number of elderly diabetics develop ketoacidosis or other serious forms of metabolic disturbance, and in these patients the mortality is high. Cardiovascular disease and peripheral vascular disease are major problems in the elderly, the former being the main cause of death. For best results, it is desirable that management of diabetes not be isolated from management of other, coexisting disorders, but rather that it be considered as part of the overall patient problem. As Malins pointed out, we should think in terms of diabetics rather than diabetes.", "PMID": 975762}
{"id": "pubmed23n0032_10472", "title": "Impotence in diabetes mellitus.", "content": "Long-standing psychogenic factors underlying impotence in diabetic males may, in many cases, play a more significant role than does prematurely presumed organic disease. With an explicit sexual history, a committed couple, an explanation of the sexual responses, and specific exchange at home, the physician may comfortably and competently intervene to provide enlightened sex therapy.", "contents": "Impotence in diabetes mellitus. Long-standing psychogenic factors underlying impotence in diabetic males may, in many cases, play a more significant role than does prematurely presumed organic disease. With an explicit sexual history, a committed couple, an explanation of the sexual responses, and specific exchange at home, the physician may comfortably and competently intervene to provide enlightened sex therapy.", "PMID": 975763}
{"id": "pubmed23n0032_10473", "title": "Diabetes mellitus and left ventricular function.", "content": "Chronic diabetes mellitus can alter left ventricular function independent of vascular effects. On the basis of available morphologic data in human and canine diabetics, alterations of myocardial interstitium may be the basis for this preclinical abnormality. The abnormal function is independent of apparent duration and treatment by diet, insulin, or hypoglycemic agents. It occurs in both sexes and is independent of age. Whether the observed functional abnormality progresses to clinical heart failure may depend on intensification of the underlying pathophysiology of the myocardium or superimposition of complications such as hypertension, obesity, and obstructive disease of the coronary vessels.", "contents": "Diabetes mellitus and left ventricular function. Chronic diabetes mellitus can alter left ventricular function independent of vascular effects. On the basis of available morphologic data in human and canine diabetics, alterations of myocardial interstitium may be the basis for this preclinical abnormality. The abnormal function is independent of apparent duration and treatment by diet, insulin, or hypoglycemic agents. It occurs in both sexes and is independent of age. Whether the observed functional abnormality progresses to clinical heart failure may depend on intensification of the underlying pathophysiology of the myocardium or superimposition of complications such as hypertension, obesity, and obstructive disease of the coronary vessels.", "PMID": 975764}
{"id": "pubmed23n0032_10474", "title": "Permanent pervenous atrial pacing.", "content": "A new J-lead has now been designed and manufactured for both animal and clinical studies. This lead has silastic tines proximal to the electrode, arranged so that they become entangled in the trabeculae of the appendage and hold the lead in place. Studies in ten dogs have shown excellent tissue fixation at the site of the tines, and only minimal scarring occurs at the site of the terminal electrode, with preservation of good P-wave amplitude and good stimulating thresholds for a period of four to five months (Figure 8, see next page). The lead designed for humans has been used successfully in 12 patients. Follow-up now extends to 19 months, with stable P-wave-triggered atrial pacing in eight patients, fixed rate atrial pacing in one, and A-V synchronized pacing in three. No lead dislodgement has occurred.", "contents": "Permanent pervenous atrial pacing. A new J-lead has now been designed and manufactured for both animal and clinical studies. This lead has silastic tines proximal to the electrode, arranged so that they become entangled in the trabeculae of the appendage and hold the lead in place. Studies in ten dogs have shown excellent tissue fixation at the site of the tines, and only minimal scarring occurs at the site of the terminal electrode, with preservation of good P-wave amplitude and good stimulating thresholds for a period of four to five months (Figure 8, see next page). The lead designed for humans has been used successfully in 12 patients. Follow-up now extends to 19 months, with stable P-wave-triggered atrial pacing in eight patients, fixed rate atrial pacing in one, and A-V synchronized pacing in three. No lead dislodgement has occurred.", "PMID": 975766}
{"id": "pubmed23n0032_10475", "title": "Chromosome anomalies in Canadian Guernsey bulls.", "content": "The performance records of a group of Guernsey bulls used for artificial insemination revealed lower 60 to 90 day non-return rates as compared to that for bulls of the Holstein breed, and nine out of the 19 Guernsey bulls in this group exhibited fertility level below the average for this group. A cytogenetic survey to test whether or not the reduced fertility of these bulls was due to chromosome anomalies revealed a relatively high frequency of chromatid breaks and achromatic gaps in three out of 19 bulls while two carried 59 chromosomes each including a translocation chromosome. The 1/29 translocation heterozygote and the bulls showing achromatic gaps and chromatid breaks were among the five bulls which were consistently below the breed average in fertility as evidenced by the t test (P less than 0.01), whereas the bull with translocation 27/29 (G3) exhibited a non-return rate slightly higher than the breed average (P less than 0.01). It is possible that the chromosomally abnormal bulls contribute to increased returns to service not only because of the defective gametes they themselves might produce but also through their influence on the performance of their daughters.", "contents": "Chromosome anomalies in Canadian Guernsey bulls. The performance records of a group of Guernsey bulls used for artificial insemination revealed lower 60 to 90 day non-return rates as compared to that for bulls of the Holstein breed, and nine out of the 19 Guernsey bulls in this group exhibited fertility level below the average for this group. A cytogenetic survey to test whether or not the reduced fertility of these bulls was due to chromosome anomalies revealed a relatively high frequency of chromatid breaks and achromatic gaps in three out of 19 bulls while two carried 59 chromosomes each including a translocation chromosome. The 1/29 translocation heterozygote and the bulls showing achromatic gaps and chromatid breaks were among the five bulls which were consistently below the breed average in fertility as evidenced by the t test (P less than 0.01), whereas the bull with translocation 27/29 (G3) exhibited a non-return rate slightly higher than the breed average (P less than 0.01). It is possible that the chromosomally abnormal bulls contribute to increased returns to service not only because of the defective gametes they themselves might produce but also through their influence on the performance of their daughters.", "PMID": 975837}
{"id": "pubmed23n0032_10476", "title": "Experimentally induced lead poisoning in goats: clinical observations and pathologic changes.", "content": "The effects of orally administered lead acetate were investigated in 9 adult and 3 kid goats by clinical and necropsy studies. One kid and 6 adults died after having received from 100 to 1392.5 Gm each, given over periods of from 10 to 52 days. Anorexia, diarrhea and body weight loss occurred in all lead treated goats in the study. Basophilic strippling of red blood cells was found in 6 of 8 animals on which weekly hemograms were performed. The pathologic changes were essentially the same as those that have been recorded for other ruminant species with lead poisoning. Intranuclar acid-fast inclusions in the cells of the proximal convulted tubules of the kidney were demonstrated in 9 of 12 lead treated goats and in the liver parenchymal cells of 2 of these animals.", "contents": "Experimentally induced lead poisoning in goats: clinical observations and pathologic changes. The effects of orally administered lead acetate were investigated in 9 adult and 3 kid goats by clinical and necropsy studies. One kid and 6 adults died after having received from 100 to 1392.5 Gm each, given over periods of from 10 to 52 days. Anorexia, diarrhea and body weight loss occurred in all lead treated goats in the study. Basophilic strippling of red blood cells was found in 6 of 8 animals on which weekly hemograms were performed. The pathologic changes were essentially the same as those that have been recorded for other ruminant species with lead poisoning. Intranuclar acid-fast inclusions in the cells of the proximal convulted tubules of the kidney were demonstrated in 9 of 12 lead treated goats and in the liver parenchymal cells of 2 of these animals.", "PMID": 975838}
{"id": "pubmed23n0032_10477", "title": "Feline calicivirus: purification of virus and extraction and characterisation of its ribonucleic acid.", "content": "Purification of feline calicivirus was achieved by cycles of differential centrifugation and two cycles of sucrose gradient centrifugation. Feline calicivirus grown in the presence of Actinomycin D and 3H-uridine-5, sediments in 15% to 45% sucrose gradients and forms a peak of radioactivity which corresponds with the peak of infectivity. Ribonucleic acid (RNA) extracted from the peak radioactive fractions taken from the sucrose gradient sedimented as a single peak ahead of the 28S peak of cellular RNA. It was sensitive to ribonuclease and was presumed to be single stranded feline calicivirus RNA with sedimentation of 32S-35S. A single peak of radioactivity at 35S was extracted from purified virus by heating at 60 degrees for two minutes in 1% sodium dodecyl sulphate (SDS), or by heating at 37 degrees for 5 minutes at 1% SDS. Virus extracted at 37 degrees for 10 minutes in 1% SDS showed also a small peak at 16S and by 15 minutes at 37 degrees only a broad peak at 16S occurred. All peaks were susceptible to ribonuclease. A component sedimenting at 18S which was resistent to degradation by ribonuclease under the conditions outlined by Baltimore (4) and presumed to be double-stranded RNA was present in kitten kidney cells infected with feline calicivirus.", "contents": "Feline calicivirus: purification of virus and extraction and characterisation of its ribonucleic acid. Purification of feline calicivirus was achieved by cycles of differential centrifugation and two cycles of sucrose gradient centrifugation. Feline calicivirus grown in the presence of Actinomycin D and 3H-uridine-5, sediments in 15% to 45% sucrose gradients and forms a peak of radioactivity which corresponds with the peak of infectivity. Ribonucleic acid (RNA) extracted from the peak radioactive fractions taken from the sucrose gradient sedimented as a single peak ahead of the 28S peak of cellular RNA. It was sensitive to ribonuclease and was presumed to be single stranded feline calicivirus RNA with sedimentation of 32S-35S. A single peak of radioactivity at 35S was extracted from purified virus by heating at 60 degrees for two minutes in 1% sodium dodecyl sulphate (SDS), or by heating at 37 degrees for 5 minutes at 1% SDS. Virus extracted at 37 degrees for 10 minutes in 1% SDS showed also a small peak at 16S and by 15 minutes at 37 degrees only a broad peak at 16S occurred. All peaks were susceptible to ribonuclease. A component sedimenting at 18S which was resistent to degradation by ribonuclease under the conditions outlined by Baltimore (4) and presumed to be double-stranded RNA was present in kitten kidney cells infected with feline calicivirus.", "PMID": 975839}
{"id": "pubmed23n0032_10478", "title": "Element and chlorinated hydrocarbon content of commercial pet foods.", "content": "An analytical survey has been conducted of 42 elements, p,p'-DDE, dieldrin andpolychlorinated biphenyls in 31 commercial canned pet foods. Neutron activation analysis, graphite furnace atomic absorption, anodic stripping voltammetry, electron affinity gas chromatography and other methods were used. Arsenic, bromine, cadmium, chromium, mercury, selenium and DDE were highest in fish-containing cat foods. Lead was most consistently high in those products containing chicken. Barium, nickel and tin also appeared high in certain of the samples.", "contents": "Element and chlorinated hydrocarbon content of commercial pet foods. An analytical survey has been conducted of 42 elements, p,p'-DDE, dieldrin andpolychlorinated biphenyls in 31 commercial canned pet foods. Neutron activation analysis, graphite furnace atomic absorption, anodic stripping voltammetry, electron affinity gas chromatography and other methods were used. Arsenic, bromine, cadmium, chromium, mercury, selenium and DDE were highest in fish-containing cat foods. Lead was most consistently high in those products containing chicken. Barium, nickel and tin also appeared high in certain of the samples.", "PMID": 975840}
{"id": "pubmed23n0032_10479", "title": "Hypercholeresis in turkeys following the ingestion of Crotalaria spectabilis seeds.", "content": "Young female turkeys received diets containing Crotalaria spectabilis seeds for 18 weeks. Mean values for total bile flow, biliary bile acid excretion, bile acid-dependent flow and bile acid-independent flow were significantly higher in crotalaria-fed turkeys than in controls. The hypercholeresis observed in crotalaria-fed birds involved both bile acid-dependent and bile acid-independent components of bile. Crotalaria-fed turkeys developed biliary hyperplasia. Liver weights were similar in the two groups. Since increases in 14C-erythritol clearances paralleled increases in total bile flow in crotalaria-fed turkeys (14C-erythritol bile to plasma ratios were similar in both groups), it was evident that the hypercholeresis in crotalaria-fed turkeys did not involve the ductal/ductular component of bile. Little or no ductal/ductular bile flow occurred in either group since total bile flow was equal to erythritol clearance and extrapolation to zero erythritol clearance yielded zero bile flow rates in both groups. Crotalaria-fed turkeys exhibited significantly higher biliary concentrations of chlorides (associated with lower sodium , potassium and bicarbonate concentrations) than did control birds. Validation of the use of 14C-erythritol clearance for the estimation of canalicular bile flow in turkeys was provided both by substantial decreases in bile to plasma 14C-erythritol concentration ratios following the injection of avian vaso-active intestinal peptide and the failure to demonstrate chromatographically the presence of radioactive metabolites of 14C-erythritol in plasma.", "contents": "Hypercholeresis in turkeys following the ingestion of Crotalaria spectabilis seeds. Young female turkeys received diets containing Crotalaria spectabilis seeds for 18 weeks. Mean values for total bile flow, biliary bile acid excretion, bile acid-dependent flow and bile acid-independent flow were significantly higher in crotalaria-fed turkeys than in controls. The hypercholeresis observed in crotalaria-fed birds involved both bile acid-dependent and bile acid-independent components of bile. Crotalaria-fed turkeys developed biliary hyperplasia. Liver weights were similar in the two groups. Since increases in 14C-erythritol clearances paralleled increases in total bile flow in crotalaria-fed turkeys (14C-erythritol bile to plasma ratios were similar in both groups), it was evident that the hypercholeresis in crotalaria-fed turkeys did not involve the ductal/ductular component of bile. Little or no ductal/ductular bile flow occurred in either group since total bile flow was equal to erythritol clearance and extrapolation to zero erythritol clearance yielded zero bile flow rates in both groups. Crotalaria-fed turkeys exhibited significantly higher biliary concentrations of chlorides (associated with lower sodium , potassium and bicarbonate concentrations) than did control birds. Validation of the use of 14C-erythritol clearance for the estimation of canalicular bile flow in turkeys was provided both by substantial decreases in bile to plasma 14C-erythritol concentration ratios following the injection of avian vaso-active intestinal peptide and the failure to demonstrate chromatographically the presence of radioactive metabolites of 14C-erythritol in plasma.", "PMID": 975841}
{"id": "pubmed23n0032_10480", "title": "Recurrent esophageal obstruction due to squamous cell carcinoma in a horse.", "content": "Squamous cell carcinoma involving the pars esophagea of the stomach caused recurrent esophageal obstruction in an 11 year old Pinto gelding. The tumor encircled the esophagus and was attached to the diaphragm, spleen, aorta, left adrenal gland and kidney. Definitive diagnosis was provided by biopsy of the mass via standing left flank laparotomy.", "contents": "Recurrent esophageal obstruction due to squamous cell carcinoma in a horse. Squamous cell carcinoma involving the pars esophagea of the stomach caused recurrent esophageal obstruction in an 11 year old Pinto gelding. The tumor encircled the esophagus and was attached to the diaphragm, spleen, aorta, left adrenal gland and kidney. Definitive diagnosis was provided by biopsy of the mass via standing left flank laparotomy.", "PMID": 975842}
{"id": "pubmed23n0032_10481", "title": "The effect of changes in blood glucose on the motility of the duodenum in sheep.", "content": "Experiments have been carried out on four adult ewes to determine the effects on duodenal motility of intravenous or intraduodenal infusion of glucose solution and intravenous injection of insulin. At blood glucose levels below 80 mg%, the mean frequency of duodenal contractions was 1.6/min, but following the establishment of hyperglycemia by either intraduodenal or intravenous glucose infusion, contraction frequency fell to 0.9/min. The strength of contraction was unaffected.", "contents": "The effect of changes in blood glucose on the motility of the duodenum in sheep. Experiments have been carried out on four adult ewes to determine the effects on duodenal motility of intravenous or intraduodenal infusion of glucose solution and intravenous injection of insulin. At blood glucose levels below 80 mg%, the mean frequency of duodenal contractions was 1.6/min, but following the establishment of hyperglycemia by either intraduodenal or intravenous glucose infusion, contraction frequency fell to 0.9/min. The strength of contraction was unaffected.", "PMID": 975843}
{"id": "pubmed23n0032_10482", "title": "Expired gas monitoring by mass spectrometry in a respiratory intensive care unit.", "content": "The application of a medical mass spectrometer for the monitoring of respired gases in the respiratory intensive care unit of a community hospital is reviewed. This monitoring system is routinely used with intubated patients for periodic monitoring of end-tidal CO2 tensions (PETCO2), FIO2, and PETO2 dead space to tidal volume ratios, and the determination of AaDO2; the value of these measurements is discussed. It is especially useful for continuous monitoring at critical points in the patient's course such as weaning from the ventilator, determining optimal ventilator settings, monitoring, unstable nonintubated patients, and in better defining the pathophysiological disturbances impeding patient progress, examples of which are presented. Preliminary observations suggest it may also provide a simple technique for determining optimal expiratory retard settings. The initial cost of such a system is justified by the benefit to the patient, i.e., reduction in the frequency of nonessential arterial blood gas determinations, shortened weaning period, and early detection of potentially dangerous trends. Technical problems encountered with this system and potential future uses are also discussed.", "contents": "Expired gas monitoring by mass spectrometry in a respiratory intensive care unit. The application of a medical mass spectrometer for the monitoring of respired gases in the respiratory intensive care unit of a community hospital is reviewed. This monitoring system is routinely used with intubated patients for periodic monitoring of end-tidal CO2 tensions (PETCO2), FIO2, and PETO2 dead space to tidal volume ratios, and the determination of AaDO2; the value of these measurements is discussed. It is especially useful for continuous monitoring at critical points in the patient's course such as weaning from the ventilator, determining optimal ventilator settings, monitoring, unstable nonintubated patients, and in better defining the pathophysiological disturbances impeding patient progress, examples of which are presented. Preliminary observations suggest it may also provide a simple technique for determining optimal expiratory retard settings. The initial cost of such a system is justified by the benefit to the patient, i.e., reduction in the frequency of nonessential arterial blood gas determinations, shortened weaning period, and early detection of potentially dangerous trends. Technical problems encountered with this system and potential future uses are also discussed.", "PMID": 975846}
{"id": "pubmed23n0032_10483", "title": "Mass spectrometry for innovative techniques of respirator care, ventilator weaning and differential ventilation in an intensive care unit.", "content": "The medical mass spectrometry as a monitoring instrument in a respiratory intensive care unit (RICU) is described. Its uses, both routine and as a tool for innovative techniques in respiratory care, are many. As an adjunct to traditional and intermittent mandatory ventilation (IMV) weaning techniques, monitoring of expired respiratory gases can hasten the safe removal of patients from mechanical ventilators. A specialized technique of dealing with ventilating the lungs differentially when their mechanical properties differ is described to illustrate the versatility of the instrument. In critical care areas, monitoring of patients with respiratory problems should include continuous monitoring of end-tidal or expired CO2.", "contents": "Mass spectrometry for innovative techniques of respirator care, ventilator weaning and differential ventilation in an intensive care unit. The medical mass spectrometry as a monitoring instrument in a respiratory intensive care unit (RICU) is described. Its uses, both routine and as a tool for innovative techniques in respiratory care, are many. As an adjunct to traditional and intermittent mandatory ventilation (IMV) weaning techniques, monitoring of expired respiratory gases can hasten the safe removal of patients from mechanical ventilators. A specialized technique of dealing with ventilating the lungs differentially when their mechanical properties differ is described to illustrate the versatility of the instrument. In critical care areas, monitoring of patients with respiratory problems should include continuous monitoring of end-tidal or expired CO2.", "PMID": 975848}
{"id": "pubmed23n0032_10484", "title": "Mass spectrometry: application in the exercise sciences.", "content": "This paper outlines a computerized system for monitoring physiological functions during exercise, using a mass spectrometer for analyzing the concentration of the mixed expired air. Examples are provided illustrating the operation of this system in both research and clinical situations. The most remarkable aspect of the system is its versatility, coupled with its accuracy. While the applications reviewed in this paper are rather narrow, work has been initiated using the mass spectrometer to measure residual lung volumes by the oxygen dilution technique, cardiac output by the CO2 rebreathing technique, and total body density by the helium dilution technique. The application of the mass spectrometer into other areas most certainty will occur in the near future.", "contents": "Mass spectrometry: application in the exercise sciences. This paper outlines a computerized system for monitoring physiological functions during exercise, using a mass spectrometer for analyzing the concentration of the mixed expired air. Examples are provided illustrating the operation of this system in both research and clinical situations. The most remarkable aspect of the system is its versatility, coupled with its accuracy. While the applications reviewed in this paper are rather narrow, work has been initiated using the mass spectrometer to measure residual lung volumes by the oxygen dilution technique, cardiac output by the CO2 rebreathing technique, and total body density by the helium dilution technique. The application of the mass spectrometer into other areas most certainty will occur in the near future.", "PMID": 975847}
{"id": "pubmed23n0032_10485", "title": "Factors influencing successful discontinuance of mechanical ventilation after open heart surgery: a clinical study of 41 patients.", "content": "Circulatory, respiratory, and metabolic variables were measured with a mobile clinical bedside unit in 41 patients during the first 48 hours after open heart surgery. Calculations were carried out off-line by a computer program. The variables were measured during controlled mechanical ventilation and compared with those obtained during spontaneous breathing and after resumption of mechanical ventilation; attempts at spontaneous breathing were categorized as successful or unsuccessful. The variables were compared before, during, and after the successful and the unsuccessful attempts at spontaneous breathing. In the series as a whole, the onset of spontaneous breathing was characterized by increases in cardiac output, radial arterial and pulmonary arterial pressures, and mixed venous oxygen tension (PVO2) and content (CVO2), as well as diminished arterial and mixed venous oxygen content differences (avDO2); no significant changes in oxygen consumption (VO2) were seen. Unsuccessful attempts disturbed the patient's physiological equilibrium by reducing oxygen delivery and not increasing VO2, while increasing ventilatory work. In general, resumption of controlled ventilation restored the physiological variables to their control conditions. Successful attempts at spontaneous breathing did not greatly affect the physiological variables. The indication for resumption of controlled ventilation after periods of spontaneous breathing is the combination of increased ventilatory work with diminished circulatory and respiratory functions.", "contents": "Factors influencing successful discontinuance of mechanical ventilation after open heart surgery: a clinical study of 41 patients. Circulatory, respiratory, and metabolic variables were measured with a mobile clinical bedside unit in 41 patients during the first 48 hours after open heart surgery. Calculations were carried out off-line by a computer program. The variables were measured during controlled mechanical ventilation and compared with those obtained during spontaneous breathing and after resumption of mechanical ventilation; attempts at spontaneous breathing were categorized as successful or unsuccessful. The variables were compared before, during, and after the successful and the unsuccessful attempts at spontaneous breathing. In the series as a whole, the onset of spontaneous breathing was characterized by increases in cardiac output, radial arterial and pulmonary arterial pressures, and mixed venous oxygen tension (PVO2) and content (CVO2), as well as diminished arterial and mixed venous oxygen content differences (avDO2); no significant changes in oxygen consumption (VO2) were seen. Unsuccessful attempts disturbed the patient's physiological equilibrium by reducing oxygen delivery and not increasing VO2, while increasing ventilatory work. In general, resumption of controlled ventilation restored the physiological variables to their control conditions. Successful attempts at spontaneous breathing did not greatly affect the physiological variables. The indication for resumption of controlled ventilation after periods of spontaneous breathing is the combination of increased ventilatory work with diminished circulatory and respiratory functions.", "PMID": 975853}
{"id": "pubmed23n0032_10486", "title": "The measurement of myocardial tissue gas tensions by mass spectrometry.", "content": "The mass spectrometer is a well established tool for the measurement of the partial pressures of gases in respiratory gas mixtures and in flowing blood. Only recently has mass spectrometry been applied to the measurement of gas tensions in tissue. Both blood and tissue gas tension measurements by mass spectrometry were made possible by the development of a special sampling catheter surrounded at its tip by a gas--but not liquid--permeable membrane. The partial pressure of each gas at the external surface of the membrane determines the flow of that gas across the membrane which, in turn, is measured by the mass spectrometer. The use of a Teflon membrane permits tissue measurements because its low permeability to gases reduces the sampling rate of gas molecules with the result that the depletion of gas molecules in the tissue surrounding the membrane is minimal. Mass spectrometry is the only technique whereby it is possible to measure regional tissue pO2 and pCO2 and other gas tensions simultaneously and continuously. One application of mass spectrometric tissue gas tension measurements is the monitoring of myocardial ischemia through measurements of myocardial tissue pO2 and pCO2. We have reviewed the work of others and ourselves in this field.", "contents": "The measurement of myocardial tissue gas tensions by mass spectrometry. The mass spectrometer is a well established tool for the measurement of the partial pressures of gases in respiratory gas mixtures and in flowing blood. Only recently has mass spectrometry been applied to the measurement of gas tensions in tissue. Both blood and tissue gas tension measurements by mass spectrometry were made possible by the development of a special sampling catheter surrounded at its tip by a gas--but not liquid--permeable membrane. The partial pressure of each gas at the external surface of the membrane determines the flow of that gas across the membrane which, in turn, is measured by the mass spectrometer. The use of a Teflon membrane permits tissue measurements because its low permeability to gases reduces the sampling rate of gas molecules with the result that the depletion of gas molecules in the tissue surrounding the membrane is minimal. Mass spectrometry is the only technique whereby it is possible to measure regional tissue pO2 and pCO2 and other gas tensions simultaneously and continuously. One application of mass spectrometric tissue gas tension measurements is the monitoring of myocardial ischemia through measurements of myocardial tissue pO2 and pCO2. We have reviewed the work of others and ourselves in this field.", "PMID": 975851}
{"id": "pubmed23n0032_10487", "title": "Imipramine (Tofranil) intoxication: a case report and review of management.", "content": "A fatal case of imipramine intoxication is reported. The patient presented with the typical findings of imipramine poisoning: coma, fixed dilated pupils, cardiac disturbances, and hyperpyrexia which, at first, were attributed to head trauma. The importance of prompt recognition of such poisoning is emphasized, and a general scheme of treatment is outlined.", "contents": "Imipramine (Tofranil) intoxication: a case report and review of management. A fatal case of imipramine intoxication is reported. The patient presented with the typical findings of imipramine poisoning: coma, fixed dilated pupils, cardiac disturbances, and hyperpyrexia which, at first, were attributed to head trauma. The importance of prompt recognition of such poisoning is emphasized, and a general scheme of treatment is outlined.", "PMID": 975855}
{"id": "pubmed23n0032_10488", "title": "Automated respiratory gas monitoring of critically injured patients.", "content": "Since 1971, we have used a mass spectrometer system for automatic hourly sampling of airway gases on a 24-hour basis in our 12 bed Critical Care Unit. Used in conjunction with arterial and mixed-venous blood samples, the availability of end-tidal O2 and CO2 values allows early identification of increasing AaDO2 and aADCO2 gradients. The ability to monitor end-tidal CO2 allows the monitoring of the adequacy of alveolar ventilation both in patients on and following removal from mechanical ventilation. Continuous information of the end-tidal PCO2 is of particular value in the management of patients with severe head injury.", "contents": "Automated respiratory gas monitoring of critically injured patients. Since 1971, we have used a mass spectrometer system for automatic hourly sampling of airway gases on a 24-hour basis in our 12 bed Critical Care Unit. Used in conjunction with arterial and mixed-venous blood samples, the availability of end-tidal O2 and CO2 values allows early identification of increasing AaDO2 and aADCO2 gradients. The ability to monitor end-tidal CO2 allows the monitoring of the adequacy of alveolar ventilation both in patients on and following removal from mechanical ventilation. Continuous information of the end-tidal PCO2 is of particular value in the management of patients with severe head injury.", "PMID": 975852}
{"id": "pubmed23n0032_10489", "title": "Complications of long tracheostomy tubes.", "content": "Complications of long tracheostomy tubes are not generally appreciated. We report three cases which illustrate that long tracheostomy tubes may impinge on the carina or, less commonly, intubate the right main-stem bronchus, with resultant atelectasis of the left lung. Since larger diameter tracheostomy tubes are also longer, they are much more likely than narrow tracheostomy tubes to produce this complication. The tracheostomy tube position should be checked frequently, especially in patients who develop atelectasis and pneumonia of the left lung.", "contents": "Complications of long tracheostomy tubes. Complications of long tracheostomy tubes are not generally appreciated. We report three cases which illustrate that long tracheostomy tubes may impinge on the carina or, less commonly, intubate the right main-stem bronchus, with resultant atelectasis of the left lung. Since larger diameter tracheostomy tubes are also longer, they are much more likely than narrow tracheostomy tubes to produce this complication. The tracheostomy tube position should be checked frequently, especially in patients who develop atelectasis and pneumonia of the left lung.", "PMID": 975854}
{"id": "pubmed23n0032_10490", "title": "A pediatric flexible fiberoptic endoscope.", "content": "Clinical experience with a flexible fiberoptic endoscope constructed for children is described. The endoscope fits through a 3.5 mm endotracheal tube without removing the tube adaptor. The endoscope was used on artificially ventilated newborns and infants for determining the position of the endotracheal tube, the integrity of the tracheal mucosal wall, and possible obstruction of the endotracheal tube. Visualization of the interior of the tube and the airways can be performed without moving the infant from the incubator. The instrument aids in the management of children requiring prolonged respiratory support. The most common lesion detected was trauma to the tracheal mucosal wall by the suction catheter.", "contents": "A pediatric flexible fiberoptic endoscope. Clinical experience with a flexible fiberoptic endoscope constructed for children is described. The endoscope fits through a 3.5 mm endotracheal tube without removing the tube adaptor. The endoscope was used on artificially ventilated newborns and infants for determining the position of the endotracheal tube, the integrity of the tracheal mucosal wall, and possible obstruction of the endotracheal tube. Visualization of the interior of the tube and the airways can be performed without moving the infant from the incubator. The instrument aids in the management of children requiring prolonged respiratory support. The most common lesion detected was trauma to the tracheal mucosal wall by the suction catheter.", "PMID": 975856}
{"id": "pubmed23n0032_10491", "title": "Comparison of G-, Q-, and R-banding in 28 cases of chromosomal abnormalities.", "content": "Twenty-eight cases of chromosomal abnormalities were ascertained using G-banding. Seventeen of these had structural abnormalities of a complex nature and are discussed in detail. An independent assessment of chromosome abnormalities was carried out using sequential Q- and R-banding. In no case was there a difference in the identification of the abnormal chromosome, but in two cases a more precise localization or definition of the abnormality was obtained from the R-banded cells. In one case the initial diagnosis of the terminal deletion was altered to interstitial deletion; in the second case a break point in one chromosome involved in a reciprocal translocation was found to be in a different band by R-banding. In several others better delineation of break points or confirmation of complex abnormalities was obtained from the R-banded cells. R-banding was especially helpful in the localization of break points because of the color differentiation obtained with acridine orange. Q-banding was not found to have added any additional information. It was concluded from this study that the use of both G-banding and R-banding in complex abnormalities proved worthwhile.", "contents": "Comparison of G-, Q-, and R-banding in 28 cases of chromosomal abnormalities. Twenty-eight cases of chromosomal abnormalities were ascertained using G-banding. Seventeen of these had structural abnormalities of a complex nature and are discussed in detail. An independent assessment of chromosome abnormalities was carried out using sequential Q- and R-banding. In no case was there a difference in the identification of the abnormal chromosome, but in two cases a more precise localization or definition of the abnormality was obtained from the R-banded cells. In one case the initial diagnosis of the terminal deletion was altered to interstitial deletion; in the second case a break point in one chromosome involved in a reciprocal translocation was found to be in a different band by R-banding. In several others better delineation of break points or confirmation of complex abnormalities was obtained from the R-banded cells. R-banding was especially helpful in the localization of break points because of the color differentiation obtained with acridine orange. Q-banding was not found to have added any additional information. It was concluded from this study that the use of both G-banding and R-banding in complex abnormalities proved worthwhile.", "PMID": 975933}
{"id": "pubmed23n0032_10492", "title": "Giemsa-banding and the identification of the Y/autosome translocation in the african marsh mongoose, Atilax paludinosus (Carnivora, Viverridae).", "content": "The diploid chromosome number of 35 in the male and 36 in the female African marsh mongoose, Atilax paludinosus, has been confirmed. C- and G-banding analyses have shown that the Y chromosome is probably translocated onto the proximal end of the acrocentric partner of a heteromorphic autosomal pair (C3). The other partner is a subtelocentric with a heterochromatic short arm. During the translocation process, this short arm was removed and presumably lost. The sex determining mechanism in Atilax could be written as XX in the female and XYA-A in the male.", "contents": "Giemsa-banding and the identification of the Y/autosome translocation in the african marsh mongoose, Atilax paludinosus (Carnivora, Viverridae). The diploid chromosome number of 35 in the male and 36 in the female African marsh mongoose, Atilax paludinosus, has been confirmed. C- and G-banding analyses have shown that the Y chromosome is probably translocated onto the proximal end of the acrocentric partner of a heteromorphic autosomal pair (C3). The other partner is a subtelocentric with a heterochromatic short arm. During the translocation process, this short arm was removed and presumably lost. The sex determining mechanism in Atilax could be written as XX in the female and XYA-A in the male.", "PMID": 975934}
{"id": "pubmed23n0032_10493", "title": "Location of nucleolar organizing regions on the chromosomes of the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus).", "content": "Terminal chromosome associations (\"satellite associations\") in ASG-banded preparations have been used to determine the number and location of staellites in the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Five pairs of satellites are found in the former and four in the latter. Nucleolar organizing regions (NORs) were visualized with the Ag-AS silver precipitation technique, and their number and position corresponded exactly with the number and position of satellites in Phodopus, where positive chromosome identification can be made in the absence of banding. Numerical agreement is exact in Mesocricetus as well, and the morphology of the silver-tagged NOR-bearing chromosomes strongly suggests that corrrespondence also occurs in this species.", "contents": "Location of nucleolar organizing regions on the chromosomes of the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Terminal chromosome associations (\"satellite associations\") in ASG-banded preparations have been used to determine the number and location of staellites in the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Five pairs of satellites are found in the former and four in the latter. Nucleolar organizing regions (NORs) were visualized with the Ag-AS silver precipitation technique, and their number and position corresponded exactly with the number and position of satellites in Phodopus, where positive chromosome identification can be made in the absence of banding. Numerical agreement is exact in Mesocricetus as well, and the morphology of the silver-tagged NOR-bearing chromosomes strongly suggests that corrrespondence also occurs in this species.", "PMID": 975935}
{"id": "pubmed23n0032_10494", "title": "Chiasmata, meiotic univalents, and age in relation to aneuploid imbalance in mice.", "content": "Chiamata were counted and paired and unpaired configurations at first meiotic division and chromosome errors at second meiotic division were assessed at different ages in males and females of two strains of laboratory mice. In the females a decrease of chiasma frequency and an increase of univalents at first meiotic metaphase (MI) were confirmed. In the males, diakineses had higher chiasma frequencies (in the range of the female MIs) and fewer univalents than the MIs had. In these male cells there was no decrease of chiasmata or increase of autosomal univalents with age, and there were some interstrain differences. In the older females there was no parallelism between the frequencies of univalents at MI and the chromosome errors that could be identified at second meiotic division; these were fewer than might be expected on the assumption that all the univalents were true univalents. The relevance of this finding to the question of the nature of most of the univalents observed at first meiotic division in aged female mice is discussed.", "contents": "Chiasmata, meiotic univalents, and age in relation to aneuploid imbalance in mice. Chiamata were counted and paired and unpaired configurations at first meiotic division and chromosome errors at second meiotic division were assessed at different ages in males and females of two strains of laboratory mice. In the females a decrease of chiasma frequency and an increase of univalents at first meiotic metaphase (MI) were confirmed. In the males, diakineses had higher chiasma frequencies (in the range of the female MIs) and fewer univalents than the MIs had. In these male cells there was no decrease of chiasmata or increase of autosomal univalents with age, and there were some interstrain differences. In the older females there was no parallelism between the frequencies of univalents at MI and the chromosome errors that could be identified at second meiotic division; these were fewer than might be expected on the assumption that all the univalents were true univalents. The relevance of this finding to the question of the nature of most of the univalents observed at first meiotic division in aged female mice is discussed.", "PMID": 975936}
{"id": "pubmed23n0032_10495", "title": "A child with presumptive monosomy 21 (45,XY,-21) in a family in which some members are Gq-.", "content": "Presumptive monosomy for chromosome 21 was found in a male child with multiple malformations and severe psychomotor retardation. Chromosome analyses of cells from blood and skin samples were performed at intervals during the first few years of his life. In preparations stained with nonbanding was well as quinacrine, Giemsa, and reverse acridine orange banding techniques, only one No. 21 chromosome could be detected with no apparent abnormalities of the other chromosomes. The proband's phenotypically normal father, paternal grandfather, brother, and paternal aunt have a deletion for a short segment of the long arm of a G-group chromosome. Genetic-marker studies allow the exclusion of a number of blood groups as being associated with No 21. There is inconclusive evidence suggesting that expression of the Duffy blood group, which has been mapped to chromosome 1, may be influenced by genetic information on chromosome 21. This family is of potential value for further gene-mapping studies.", "contents": "A child with presumptive monosomy 21 (45,XY,-21) in a family in which some members are Gq-. Presumptive monosomy for chromosome 21 was found in a male child with multiple malformations and severe psychomotor retardation. Chromosome analyses of cells from blood and skin samples were performed at intervals during the first few years of his life. In preparations stained with nonbanding was well as quinacrine, Giemsa, and reverse acridine orange banding techniques, only one No. 21 chromosome could be detected with no apparent abnormalities of the other chromosomes. The proband's phenotypically normal father, paternal grandfather, brother, and paternal aunt have a deletion for a short segment of the long arm of a G-group chromosome. Genetic-marker studies allow the exclusion of a number of blood groups as being associated with No 21. There is inconclusive evidence suggesting that expression of the Duffy blood group, which has been mapped to chromosome 1, may be influenced by genetic information on chromosome 21. This family is of potential value for further gene-mapping studies.", "PMID": 975937}
{"id": "pubmed23n0032_10496", "title": "Chromosome banding pattern relationships of hares, rabbits, and pikas (order Lagomorpha). A phyletic interpretation.", "content": "The chromosome banding patterns of the domestic rabbit, Oryctolagus cuniculus, the brush rabbit, Sylvilagus bachmani, and the snowshoe hare, Lepus americanus, are nearly identical except for minor differences in the amount of constitutive heterochromatin present. The major mechanisms of chromosome evolution in leporid lagomorphs are centric fusion and addition of heterochromatin. The G- and C-banding patterns of the pika, Ochotona princeps, revealed little easily recognized structural homology with the leporid species. Several tentative banding pattern matches were found for segments of some chromosomes of Ochotona, indicating that tandem fusions may have been responsible for structural modification of the early lagomorph genome. Chromosome evolution in the lagomorphs and the use of chromosomal data in studies of mammalian systematics are discussed.", "contents": "Chromosome banding pattern relationships of hares, rabbits, and pikas (order Lagomorpha). A phyletic interpretation. The chromosome banding patterns of the domestic rabbit, Oryctolagus cuniculus, the brush rabbit, Sylvilagus bachmani, and the snowshoe hare, Lepus americanus, are nearly identical except for minor differences in the amount of constitutive heterochromatin present. The major mechanisms of chromosome evolution in leporid lagomorphs are centric fusion and addition of heterochromatin. The G- and C-banding patterns of the pika, Ochotona princeps, revealed little easily recognized structural homology with the leporid species. Several tentative banding pattern matches were found for segments of some chromosomes of Ochotona, indicating that tandem fusions may have been responsible for structural modification of the early lagomorph genome. Chromosome evolution in the lagomorphs and the use of chromosomal data in studies of mammalian systematics are discussed.", "PMID": 975938}
{"id": "pubmed23n0032_10497", "title": "Reduction of blood usage in open heart surgery.", "content": "A series of 142 adult patients undergoing open-heart surgery were studied. All known blood-conservativing methods were utilized in an attempt to use as little blood as possible. Hemodilution, autologous transfusion, prevention of wasting of blood, and management of postoperative anemia were the measures employed. An average of 2.66 units of blood were given per patient during the entire hospital stay. Twenty patients were not given any blood at all. The patients were removed from cardiopulmonary bypass without difficulty when the hematocrit reading was in the high teens or low twenties. Later in the postoperative period the patients seemed to progress without difficulty with hematocrit readings of 22 to 25 percent.", "contents": "Reduction of blood usage in open heart surgery. A series of 142 adult patients undergoing open-heart surgery were studied. All known blood-conservativing methods were utilized in an attempt to use as little blood as possible. Hemodilution, autologous transfusion, prevention of wasting of blood, and management of postoperative anemia were the measures employed. An average of 2.66 units of blood were given per patient during the entire hospital stay. Twenty patients were not given any blood at all. The patients were removed from cardiopulmonary bypass without difficulty when the hematocrit reading was in the high teens or low twenties. Later in the postoperative period the patients seemed to progress without difficulty with hematocrit readings of 22 to 25 percent.", "PMID": 975949}
{"id": "pubmed23n0032_10498", "title": "Diagnosis of gastric aspiration by fiberoptic bronchoscopy.", "content": "At the time of bronchoscopic observation, 28 of 31 patients proved to have aspirated gastric contents by the presence of gastric contents in the trachea or by direct observation of gastric aspiration followed by respiratory distress and erythema of subsegmental bronchi. Since gastric aspiration is a difficult diagnosis to make and few objective data are available to make the diagnosis, the present study would indicate that early fiberoptic bronchoscopic observation of the tracheobronchial tree is a useful procedure to establish a definitive diagnosis and to allow proper planning for clinical management.", "contents": "Diagnosis of gastric aspiration by fiberoptic bronchoscopy. At the time of bronchoscopic observation, 28 of 31 patients proved to have aspirated gastric contents by the presence of gastric contents in the trachea or by direct observation of gastric aspiration followed by respiratory distress and erythema of subsegmental bronchi. Since gastric aspiration is a difficult diagnosis to make and few objective data are available to make the diagnosis, the present study would indicate that early fiberoptic bronchoscopic observation of the tracheobronchial tree is a useful procedure to establish a definitive diagnosis and to allow proper planning for clinical management.", "PMID": 975950}
{"id": "pubmed23n0032_10499", "title": "Familial supravalvular aortic stenosis. Report of a large family and review of the literature.", "content": "Familial supravalvular aortic stenosis has been recognized as a distinct syndrome. A large family with five proven cases and a review of the literature on familial supravalvular aortic stenosis are presented. The diagnosis was substantiated in all 63 cases by cardiac catheterization, surgery, or postmortem examination.", "contents": "Familial supravalvular aortic stenosis. Report of a large family and review of the literature. Familial supravalvular aortic stenosis has been recognized as a distinct syndrome. A large family with five proven cases and a review of the literature on familial supravalvular aortic stenosis are presented. The diagnosis was substantiated in all 63 cases by cardiac catheterization, surgery, or postmortem examination.", "PMID": 975952}
{"id": "pubmed23n0032_10500", "title": "Effect of emotional stress on platelet aggregation in humans.", "content": "In 16 young house physicians, each of whom presented a case at grand rounds, the effect on platelet function of the emotional stress involved in presenting the case was determined. Platelet-aggregation curves, using adenosine diphosphate and epinephrine, were determined immediately before, immediately after, and seven days after stress. Platelet aggregation was diminished during the stress period in 15 of the subjects. These findings may possibly be of importance in the relationship between stress and myocardial infarction; they also suggest that the emotional state of the patient must be considered when interpreting platelet-aggregation curves.", "contents": "Effect of emotional stress on platelet aggregation in humans. In 16 young house physicians, each of whom presented a case at grand rounds, the effect on platelet function of the emotional stress involved in presenting the case was determined. Platelet-aggregation curves, using adenosine diphosphate and epinephrine, were determined immediately before, immediately after, and seven days after stress. Platelet aggregation was diminished during the stress period in 15 of the subjects. These findings may possibly be of importance in the relationship between stress and myocardial infarction; they also suggest that the emotional state of the patient must be considered when interpreting platelet-aggregation curves.", "PMID": 975953}
{"id": "pubmed23n0032_10501", "title": "Unusual electrocardiographic changes in spontaneous pneumothorax.", "content": "A young woman with spontaneous left pneumothorax had a phasic voltage alternation of her electrocardiogram that resolved with expansion of the lung. Likely explanations for this phenomenon are a respiratory dependent change in cardiac anatomy and a change in the volume conductor with respiration.", "contents": "Unusual electrocardiographic changes in spontaneous pneumothorax. A young woman with spontaneous left pneumothorax had a phasic voltage alternation of her electrocardiogram that resolved with expansion of the lung. Likely explanations for this phenomenon are a respiratory dependent change in cardiac anatomy and a change in the volume conductor with respiration.", "PMID": 975956}
{"id": "pubmed23n0032_10502", "title": "Endobronchial tuberculosis progressing to bronchial stenosis. Fiberoptic bronchoscopic manifestations.", "content": "Severe stenosis of main-stem and segmental bronchi occurred in a 79-year-old woman with previously and adequately treated endobronchial tuberculosis. Symptoms and physiologic abnormalities did not occur until more than three years after diagnosis and initiation of therapy.", "contents": "Endobronchial tuberculosis progressing to bronchial stenosis. Fiberoptic bronchoscopic manifestations. Severe stenosis of main-stem and segmental bronchi occurred in a 79-year-old woman with previously and adequately treated endobronchial tuberculosis. Symptoms and physiologic abnormalities did not occur until more than three years after diagnosis and initiation of therapy.", "PMID": 975957}
{"id": "pubmed23n0032_10503", "title": "Congenital complete atrioventricular block and prolapsing mitral valve.", "content": "Congenital complete atrioventricular block and prolapsing mitral valve are described in a 41-year-old woman who exhibited ventricular arrhythmias and symptoms initially attributed to her atrioventricular block. The importance of physical examination to elicit clues in the diagnosis of prolapsing mitral valve is emphasized.", "contents": "Congenital complete atrioventricular block and prolapsing mitral valve. Congenital complete atrioventricular block and prolapsing mitral valve are described in a 41-year-old woman who exhibited ventricular arrhythmias and symptoms initially attributed to her atrioventricular block. The importance of physical examination to elicit clues in the diagnosis of prolapsing mitral valve is emphasized.", "PMID": 975958}
{"id": "pubmed23n0032_10504", "title": "Surgical complications of cervical and mediastinal tuberculous adenitis in an infant.", "content": "An infant whose miliary tuberculosis was resolving after five months of chemotherapy developed a tuberculous cervical lymphatic abscess followed shortly thereafter by life-threatening tracheal compression from enlarged tuberculous mediastinal nodes. Both of these complications required surgical drainage. Chemotherapy alone may be ineffective in the management of lymphadenitis caused by Mycobacterium tuberculosis.", "contents": "Surgical complications of cervical and mediastinal tuberculous adenitis in an infant. An infant whose miliary tuberculosis was resolving after five months of chemotherapy developed a tuberculous cervical lymphatic abscess followed shortly thereafter by life-threatening tracheal compression from enlarged tuberculous mediastinal nodes. Both of these complications required surgical drainage. Chemotherapy alone may be ineffective in the management of lymphadenitis caused by Mycobacterium tuberculosis.", "PMID": 975959}
{"id": "pubmed23n0032_10505", "title": "Tracheoesophageal and aortoesophageal fistulae complicating corrosive esophagitis.", "content": "A 51-year-old man suffered third-degree caustic burns of the esophagus and proximal two-thirds of the stomach. A tracheoesophageal fistula developed, and surgery was performed to exclude the esophagus from gastrointestinal reflux and to divert the oral secretions. An uneventful postoperative course was interrupted on the 27th day after the burn by massive hemoptysis and sudden death. Autopsy revealed an aortoesophageal fistula in addition to a tracheoesophageal fistula.", "contents": "Tracheoesophageal and aortoesophageal fistulae complicating corrosive esophagitis. A 51-year-old man suffered third-degree caustic burns of the esophagus and proximal two-thirds of the stomach. A tracheoesophageal fistula developed, and surgery was performed to exclude the esophagus from gastrointestinal reflux and to divert the oral secretions. An uneventful postoperative course was interrupted on the 27th day after the burn by massive hemoptysis and sudden death. Autopsy revealed an aortoesophageal fistula in addition to a tracheoesophageal fistula.", "PMID": 975960}
{"id": "pubmed23n0032_10506", "title": "Posthyperventilation apnea associated with severe hypoxemia.", "content": "We studied a 14-year-old girl who suffered fractures of her mandible and tegmen following a fall from a balance beam. Thirteen days after hospitalization, she developed severe, protracted, recurrent episodes of hyperventilation; subsequently, she suffered posthyperventilation apnea, which at times was prolonged and association with severe hypoxemia with an arterial oxygen pressure as low as 25 mm Hg. The patient was treated with added dead space and chlorpromazine hydrochloride (Thorazine). Postulated mechanisms for her disorder are discussed. The importance of close clinical and laboratory observation in similar cases is stressed.", "contents": "Posthyperventilation apnea associated with severe hypoxemia. We studied a 14-year-old girl who suffered fractures of her mandible and tegmen following a fall from a balance beam. Thirteen days after hospitalization, she developed severe, protracted, recurrent episodes of hyperventilation; subsequently, she suffered posthyperventilation apnea, which at times was prolonged and association with severe hypoxemia with an arterial oxygen pressure as low as 25 mm Hg. The patient was treated with added dead space and chlorpromazine hydrochloride (Thorazine). Postulated mechanisms for her disorder are discussed. The importance of close clinical and laboratory observation in similar cases is stressed.", "PMID": 975961}
{"id": "pubmed23n0032_10507", "title": "Acquired continuous murmur associated with acute pulmonary thromboembolism.", "content": "Two cases of a continuous murmur following an acute pulmonary embolic episode are presented, and eight previously reported cases with an acquired postembolic continuous murmur (found in a review of the literature) are discussed. This finding is present in both chronic and acute pulmonary embolism and is suggestive of significant embolic obstruction. Although the continuous murmur is an unusual sign in patients with pulmonary embolism, its auscultation is often quite distinctive, and its appearance may lead to more definitive diagnostic studies when the presentation or associated clinical findings are nonspecific.", "contents": "Acquired continuous murmur associated with acute pulmonary thromboembolism. Two cases of a continuous murmur following an acute pulmonary embolic episode are presented, and eight previously reported cases with an acquired postembolic continuous murmur (found in a review of the literature) are discussed. This finding is present in both chronic and acute pulmonary embolism and is suggestive of significant embolic obstruction. Although the continuous murmur is an unusual sign in patients with pulmonary embolism, its auscultation is often quite distinctive, and its appearance may lead to more definitive diagnostic studies when the presentation or associated clinical findings are nonspecific.", "PMID": 975962}
{"id": "pubmed23n0032_10508", "title": "Ventricular septal defect complicating acute myocardial infarction. Echocardiographic demonstration confirmed by angiocardiograms and surgery.", "content": "The echocardiographic findings in one patient with a ventricular septal perforation as a result of an acute anteroseptal myocardial infarction are presented. Continuous echocardiographic scanning enabled us to demonstrate a septal discontinuity below the atrioventricular junction. The correct echocardiographic diagnosis was confirmed angiocardiographically. After surgical repair of the ventricular septal defect, the echocardiogram failed to reveal the septal discontinuity previously present. A search of the literature did not disclose any report of similar echocardiographic findings.", "contents": "Ventricular septal defect complicating acute myocardial infarction. Echocardiographic demonstration confirmed by angiocardiograms and surgery. The echocardiographic findings in one patient with a ventricular septal perforation as a result of an acute anteroseptal myocardial infarction are presented. Continuous echocardiographic scanning enabled us to demonstrate a septal discontinuity below the atrioventricular junction. The correct echocardiographic diagnosis was confirmed angiocardiographically. After surgical repair of the ventricular septal defect, the echocardiogram failed to reveal the septal discontinuity previously present. A search of the literature did not disclose any report of similar echocardiographic findings.", "PMID": 975963}
{"id": "pubmed23n0032_10509", "title": "Myocardial infarction in younger women. Associated clinical features and relationship to use of oral contraceptive drugs.", "content": "In a study of thromboembolism and oral contraceptive drugs, 136 cases of myocardial infarction in women aged 30 to 44 years were identified, a rare disease in women of this age group. Data from their hospital medical records were compared with those of several other groups, including women without chronic disease admitted for various acute or elective conditions unrelated to thromboembolism, patients with thromboembolic disease other than myocardial infarction, and women queried or examined in the National Health Survey. The following attributes were found to be associated with myocardial infarction in younger women: presence of diabetes; hypertension; history of increased cigarette smoking; and hypercholesterolemia. A history of the use of oral contraceptive drugs was found with greater frequency in cases than in controls. The literature on the possible association of myocardial infarction and the use of oral contraceptives was reviewed.", "contents": "Myocardial infarction in younger women. Associated clinical features and relationship to use of oral contraceptive drugs. In a study of thromboembolism and oral contraceptive drugs, 136 cases of myocardial infarction in women aged 30 to 44 years were identified, a rare disease in women of this age group. Data from their hospital medical records were compared with those of several other groups, including women without chronic disease admitted for various acute or elective conditions unrelated to thromboembolism, patients with thromboembolic disease other than myocardial infarction, and women queried or examined in the National Health Survey. The following attributes were found to be associated with myocardial infarction in younger women: presence of diabetes; hypertension; history of increased cigarette smoking; and hypercholesterolemia. A history of the use of oral contraceptive drugs was found with greater frequency in cases than in controls. The literature on the possible association of myocardial infarction and the use of oral contraceptives was reviewed.", "PMID": 975971}
{"id": "pubmed23n0032_10510", "title": "Pulmonary hemorrhage in fiberoptic transbronchial biopsy.", "content": "Transbronchial forceps biopsy via the flexible fiberoptic bronchoscope is described as a \"safe,\" high-yielding procedure, but the potential danger of serious hemorrhage is of concern to chest physicians. In a collected series of 438 patients, the incidence of mild to \"explosive\" hemorrhage was 9 percent in \"routine\" cases, 29 percent (eight) in 31 immunosuppressed patients, and 45 percent (five) in 11 uremic patients. One death resulted from massive hemorrhage. A new \"wedge\" method of transbronchial forceps biopsy is now being utilized in our bronchoscopic unit. The tip of the flexible fiberoptic bronchoscope is lodged into the appropriate segmental bronchus to tamponade any bleeding and, thus, prevent blood from flooding the airway. Careful screening of patients and competence in procedural techniques are necessary. Otherwise, transbronchial forceps biopsy should be performed through a rigid open-tube bronchoscopicronchoscope or performed through a rigid open-tube bronchoscope or lung tissue should be obtained via thoracotomy.", "contents": "Pulmonary hemorrhage in fiberoptic transbronchial biopsy. Transbronchial forceps biopsy via the flexible fiberoptic bronchoscope is described as a \"safe,\" high-yielding procedure, but the potential danger of serious hemorrhage is of concern to chest physicians. In a collected series of 438 patients, the incidence of mild to \"explosive\" hemorrhage was 9 percent in \"routine\" cases, 29 percent (eight) in 31 immunosuppressed patients, and 45 percent (five) in 11 uremic patients. One death resulted from massive hemorrhage. A new \"wedge\" method of transbronchial forceps biopsy is now being utilized in our bronchoscopic unit. The tip of the flexible fiberoptic bronchoscope is lodged into the appropriate segmental bronchus to tamponade any bleeding and, thus, prevent blood from flooding the airway. Careful screening of patients and competence in procedural techniques are necessary. Otherwise, transbronchial forceps biopsy should be performed through a rigid open-tube bronchoscopicronchoscope or performed through a rigid open-tube bronchoscope or lung tissue should be obtained via thoracotomy.", "PMID": 975972}
{"id": "pubmed23n0032_10511", "title": "Flexible fiberoptic bronchoscopy and endobronchial tamponade in the management of massive hemoptysis.", "content": "Flexible fiberoptic bronchoscopic examination with systematic bronchial lavage was performed in ten patients with massive hemoptysis. Localization of the bleeding to the distal segments of the bronchial tree and endobronchial balloon tamponade were achieved in all cases. The technique is rapid, simple, and effective in controlling life-threatening hemoptysis in patients who are unsuitable for resection due to inadequate pulmonary reserve or other causes.", "contents": "Flexible fiberoptic bronchoscopy and endobronchial tamponade in the management of massive hemoptysis. Flexible fiberoptic bronchoscopic examination with systematic bronchial lavage was performed in ten patients with massive hemoptysis. Localization of the bleeding to the distal segments of the bronchial tree and endobronchial balloon tamponade were achieved in all cases. The technique is rapid, simple, and effective in controlling life-threatening hemoptysis in patients who are unsuitable for resection due to inadequate pulmonary reserve or other causes.", "PMID": 975973}
{"id": "pubmed23n0032_10512", "title": "Autologous blood transfusion during cardiopulmonary bypass.", "content": "Immediately prior to cardiopulmonary bypass, two units of blood were removed from each of 25 patients undergoing open-heart surgery, and the autologous blood was reinfused after cessation of support with the pump oxygenator.  Pertinent data on blood balance and hematologic measurements were compared to a matched group of control subjects. There were no significant differences in the amount of operative or postoperative bleeding, the requirements for homologous blood and blood products, or the amount of protamine needed for neutralization of heparin.", "contents": "Autologous blood transfusion during cardiopulmonary bypass. Immediately prior to cardiopulmonary bypass, two units of blood were removed from each of 25 patients undergoing open-heart surgery, and the autologous blood was reinfused after cessation of support with the pump oxygenator.  Pertinent data on blood balance and hematologic measurements were compared to a matched group of control subjects. There were no significant differences in the amount of operative or postoperative bleeding, the requirements for homologous blood and blood products, or the amount of protamine needed for neutralization of heparin.", "PMID": 975974}
{"id": "pubmed23n0032_10513", "title": "Natural history of unstable angina with medical or surgical therapy.", "content": "In order to establish the natural evolution of unstable angina under medical treatment and to determine the possible benefits of revascularization surgery, 113 patients were studied; 51 received medical treatment (24 with intermediate syndrome and 27 with progressive angina), and 62 others received surgical treatment (28 with intermediate syndrome and 34 with progressive angina). After a mean follow-up of 32 months, the mortality in the medically treated groups was 46 percent (11/24) with intermediate syndrome and 7 percent (2/27) with progressive angina (P less than 0.005), and the incidence of myocardial infarction was 38 percent (9/24) and 7 percent (2/27), respectively (P less than 0.01). Moreover, in comparing cases treated medically or surgically, the mortality was as follows: intermediate syndrome treated medically, 46 percent (11/24) vs 11 percent (3/28) in those treated surgically (P less than 0.005); and progressive angina treated medically, 7 percent (2/27) vs 9 percent (3/34) in those treated surgically (P = 0.85). The incidence of myocardial infarction was as follows: intermediate syndrome treated medically, 38 percent (9/24) vs 14 percent (4/28) in those treated surgically (P less than or equal to 0.056); and progressive angina treated medically, 7 percent (2/27) vs 12 percent (4/34) in those treated surgically (P greater than 0.55).", "contents": "Natural history of unstable angina with medical or surgical therapy. In order to establish the natural evolution of unstable angina under medical treatment and to determine the possible benefits of revascularization surgery, 113 patients were studied; 51 received medical treatment (24 with intermediate syndrome and 27 with progressive angina), and 62 others received surgical treatment (28 with intermediate syndrome and 34 with progressive angina). After a mean follow-up of 32 months, the mortality in the medically treated groups was 46 percent (11/24) with intermediate syndrome and 7 percent (2/27) with progressive angina (P less than 0.005), and the incidence of myocardial infarction was 38 percent (9/24) and 7 percent (2/27), respectively (P less than 0.01). Moreover, in comparing cases treated medically or surgically, the mortality was as follows: intermediate syndrome treated medically, 46 percent (11/24) vs 11 percent (3/28) in those treated surgically (P less than 0.005); and progressive angina treated medically, 7 percent (2/27) vs 9 percent (3/34) in those treated surgically (P = 0.85). The incidence of myocardial infarction was as follows: intermediate syndrome treated medically, 38 percent (9/24) vs 14 percent (4/28) in those treated surgically (P less than or equal to 0.056); and progressive angina treated medically, 7 percent (2/27) vs 12 percent (4/34) in those treated surgically (P greater than 0.55).", "PMID": 975975}
{"id": "pubmed23n0032_10514", "title": "Hospitalization needs during an outpatient rehabilitation program for severe chronic airway obstruction.", "content": "The number of days of hospitalization for respiratory disease following entry into an outpatient pulmonary rehabilitation program was reviewed for 44 patients with chronic airway obstruction whose hospitalization records were available for the year prior to entry into the program. A significant reduction in the number of days of hospitalization occurred in patients for each of the four years following entry into the program, compared to the year prior to therapy.", "contents": "Hospitalization needs during an outpatient rehabilitation program for severe chronic airway obstruction. The number of days of hospitalization for respiratory disease following entry into an outpatient pulmonary rehabilitation program was reviewed for 44 patients with chronic airway obstruction whose hospitalization records were available for the year prior to entry into the program. A significant reduction in the number of days of hospitalization occurred in patients for each of the four years following entry into the program, compared to the year prior to therapy.", "PMID": 975976}
{"id": "pubmed23n0032_10515", "title": "A comparison of two bipolar exercise electrocardiographic leads to lead V5.", "content": "ST-segment depression and slope were compared in three lead systems (V5, CC5, and CM5) and in two groups of patients using both visual analysis of electrocardiographic paper and computerized techniques. Bipolar lead CC5 was found to be comparable to lead V5 when visual analysis of electrocardiographic recordings was utilized. Bipolar lead CM5 was found not to be comparable to lead V5 and to be less sensitive if classic criteria for slope were used. The technique of computerized analysis mad measurements of slope and amplitude to a reproducible level not possible with the standard technique. Statistically significant differences were found between the exercise electrocardiographic leads utilizing computerized electrocardiographic analysis . We conclude that computerized techniques of electrocardiographic analysis require new criteria for defining an abnormal repolarization response. The criteria must be specific for different electrocardiographic leads if the repolarization changes in these leads are to have comparable diagnostic significance.", "contents": "A comparison of two bipolar exercise electrocardiographic leads to lead V5. ST-segment depression and slope were compared in three lead systems (V5, CC5, and CM5) and in two groups of patients using both visual analysis of electrocardiographic paper and computerized techniques. Bipolar lead CC5 was found to be comparable to lead V5 when visual analysis of electrocardiographic recordings was utilized. Bipolar lead CM5 was found not to be comparable to lead V5 and to be less sensitive if classic criteria for slope were used. The technique of computerized analysis mad measurements of slope and amplitude to a reproducible level not possible with the standard technique. Statistically significant differences were found between the exercise electrocardiographic leads utilizing computerized electrocardiographic analysis . We conclude that computerized techniques of electrocardiographic analysis require new criteria for defining an abnormal repolarization response. The criteria must be specific for different electrocardiographic leads if the repolarization changes in these leads are to have comparable diagnostic significance.", "PMID": 975977}
{"id": "pubmed23n0032_10516", "title": "Long-term efficacy and safety of nebulized metaproterenol solution in bronchial asthma.", "content": "A 5-percent solution of the sympathomimetic bronchodilator, metaproterenol sulfate (Alupent) was evaluated by comparison with an 0.5-percent solution of isoproterenol in a double-blind crossover study before and after 60 days of inhalation of metaproterenol administered at least four times daily via a hand-bulb nebulizer. Data from tests of pulmonary function obtained in 27 asthmatic patients indicated that metaproterenol sulfate in this dose form surpassed isoproterenol in the duration of effect after seven weeks of continuous administration. Side effects did not necessitate the interruption of metaproterenol therapy. No evidence of the development of tolerance to the drug was shown by any of the patients at the end of the study.", "contents": "Long-term efficacy and safety of nebulized metaproterenol solution in bronchial asthma. A 5-percent solution of the sympathomimetic bronchodilator, metaproterenol sulfate (Alupent) was evaluated by comparison with an 0.5-percent solution of isoproterenol in a double-blind crossover study before and after 60 days of inhalation of metaproterenol administered at least four times daily via a hand-bulb nebulizer. Data from tests of pulmonary function obtained in 27 asthmatic patients indicated that metaproterenol sulfate in this dose form surpassed isoproterenol in the duration of effect after seven weeks of continuous administration. Side effects did not necessitate the interruption of metaproterenol therapy. No evidence of the development of tolerance to the drug was shown by any of the patients at the end of the study.", "PMID": 975978}
{"id": "pubmed23n0032_10517", "title": "Coarctation of the aorta in children and adolescents. Surgical treatment and review of 120 patients.", "content": "Between September 1967 and April 1975, a total of 120 patients between one day and 20 years of age underwent surgery for coarctation of the thoracic aorta. Thirty-two patients were below two years of age (group 1) and 88 were above two years (group 3). All patients in group 1 initially had congestive heart failure. Twenty-eight had associated cardiac defects, and 18 had signficant pulmonary arterial hypertension (greater than 50 mm Hg). Operative deaths occurred only in group 1, all in infants below five months of age. Common features in the 13 deaths were congestive heart failure, pulmonary hypertension, patent ductus arteriosus, large ventricular septal defect, concomitant pulmonary arterial bandling or open-heart procedures. The only recurrence occurred in an infant first operated at 14 days of age. Resection of aortic coarctation can be safely performed as an elective procedure; however, it still presents a high surgical risk in infants with associated intracardiac defects. Late follow-up shows the salutary effects of repair of the coarctation on hypertension.", "contents": "Coarctation of the aorta in children and adolescents. Surgical treatment and review of 120 patients. Between September 1967 and April 1975, a total of 120 patients between one day and 20 years of age underwent surgery for coarctation of the thoracic aorta. Thirty-two patients were below two years of age (group 1) and 88 were above two years (group 3). All patients in group 1 initially had congestive heart failure. Twenty-eight had associated cardiac defects, and 18 had signficant pulmonary arterial hypertension (greater than 50 mm Hg). Operative deaths occurred only in group 1, all in infants below five months of age. Common features in the 13 deaths were congestive heart failure, pulmonary hypertension, patent ductus arteriosus, large ventricular septal defect, concomitant pulmonary arterial bandling or open-heart procedures. The only recurrence occurred in an infant first operated at 14 days of age. Resection of aortic coarctation can be safely performed as an elective procedure; however, it still presents a high surgical risk in infants with associated intracardiac defects. Late follow-up shows the salutary effects of repair of the coarctation on hypertension.", "PMID": 975979}
{"id": "pubmed23n0032_10518", "title": "Plug closure of patent ductus arteriosus by transfemoral catheter method. A comparative study with surgery and a new technical modification.", "content": "Plug closure of patent ductus arteriosus without thoracotomy is reliable and can be a good alternative to surgical closure for selected patients. We report our experiences with plug closure in 87 consecutive patients (age range, 3 to 38 years). Closure was successful in 83 patients (95 percent). There has been no mortality, and the failure in four patients (5 percent) was mainly due to an oversized ductus. A major complication during the procedure was dislodgment of the plug into either the aorta or the pulmonary artery, which occurred in five patients (6 percent). The late results were quite satisfactory, with no recurrence of shunting or any other complications. Comparison of these results with those of 100 patients treated surgically showed that the new catheter method did not seem to carry a higher risk but had certain advantages over thoracotomy. A simpler and less time-consuming method using a single catheter has been devised and successfully used in the most recent nine patients without failure. Simplification of the technique has signficantly reduced the time of the procedure and the dose of radiation.", "contents": "Plug closure of patent ductus arteriosus by transfemoral catheter method. A comparative study with surgery and a new technical modification. Plug closure of patent ductus arteriosus without thoracotomy is reliable and can be a good alternative to surgical closure for selected patients. We report our experiences with plug closure in 87 consecutive patients (age range, 3 to 38 years). Closure was successful in 83 patients (95 percent). There has been no mortality, and the failure in four patients (5 percent) was mainly due to an oversized ductus. A major complication during the procedure was dislodgment of the plug into either the aorta or the pulmonary artery, which occurred in five patients (6 percent). The late results were quite satisfactory, with no recurrence of shunting or any other complications. Comparison of these results with those of 100 patients treated surgically showed that the new catheter method did not seem to carry a higher risk but had certain advantages over thoracotomy. A simpler and less time-consuming method using a single catheter has been devised and successfully used in the most recent nine patients without failure. Simplification of the technique has signficantly reduced the time of the procedure and the dose of radiation.", "PMID": 975980}
{"id": "pubmed23n0032_10519", "title": "Role of selected muscles of respiration as influenced by posture and tidal volume.", "content": "Studies of the activity of accessory respiratory muscles were conducted in 30 normal subjects (15 male and 15 female subjects, age 5 to 62 years). Electromyograms were recorded over the right serratus anterior muscle and the adjacent sixthe intercostal space, and the results were correlated with tidal volume. Phasic respiratory activity in the serratus anterior muscle was detected in every subject but was markedly affected by the prevailing posture and level of ventilation (appearing at mean tidal volumes equivalent to 60.5 percent of vital capacity when subjects were standing relaxed, and equivalent to 35.2 percent of vital capacity when subjects were seated, leaning forward with elbows supported). The activity was predominantly inspiratory in timing but in some subjects extended into early expiration. No significant differences were noted in subjects of different ages, sex, height, or weight. Similar results were recorded from the inspiratory intercostal muscle. On the basis of these findings, we conclude that, contrary to reports, the serratus anterior muscle should be classified as an accessory muscle of respiration, at least at increased levels of ventilation, and that its activity is most pronounced in postures that place the muscles' origin in an advantageous position for moving the ribs.", "contents": "Role of selected muscles of respiration as influenced by posture and tidal volume. Studies of the activity of accessory respiratory muscles were conducted in 30 normal subjects (15 male and 15 female subjects, age 5 to 62 years). Electromyograms were recorded over the right serratus anterior muscle and the adjacent sixthe intercostal space, and the results were correlated with tidal volume. Phasic respiratory activity in the serratus anterior muscle was detected in every subject but was markedly affected by the prevailing posture and level of ventilation (appearing at mean tidal volumes equivalent to 60.5 percent of vital capacity when subjects were standing relaxed, and equivalent to 35.2 percent of vital capacity when subjects were seated, leaning forward with elbows supported). The activity was predominantly inspiratory in timing but in some subjects extended into early expiration. No significant differences were noted in subjects of different ages, sex, height, or weight. Similar results were recorded from the inspiratory intercostal muscle. On the basis of these findings, we conclude that, contrary to reports, the serratus anterior muscle should be classified as an accessory muscle of respiration, at least at increased levels of ventilation, and that its activity is most pronounced in postures that place the muscles' origin in an advantageous position for moving the ribs.", "PMID": 975981}
{"id": "pubmed23n0032_10520", "title": "Severe bullous emphysema. Successful surgical management despite poor preoperative blood gas levels and marked pulmonary hypertension.", "content": "Surgical removal of giant pulmonary bullae is usually considered inadvisable in patients with either hypoxemia or hypercapnia. A successful operation was performed on a patient with both hypoxemia and hypercapnia. Preoperative and postoperative physiologic data are presented. Those data which suggested a favorable outcome are compared with those which did not.", "contents": "Severe bullous emphysema. Successful surgical management despite poor preoperative blood gas levels and marked pulmonary hypertension. Surgical removal of giant pulmonary bullae is usually considered inadvisable in patients with either hypoxemia or hypercapnia. A successful operation was performed on a patient with both hypoxemia and hypercapnia. Preoperative and postoperative physiologic data are presented. Those data which suggested a favorable outcome are compared with those which did not.", "PMID": 975985}
{"id": "pubmed23n0032_10521", "title": "Double prosthetic aortic valve. Late aortic erosion by Hufnagel valve.", "content": "This is a case report of a patient who had one of the first Hufnagel ball-valve prostheses inserted in 1954 for severe aortic incompetence. He obtained relief of symptoms for 14 years before recurrence necessitated the insertion of a Starr-Edwards valve. The patients was symptom-free for another seven years before the original valve, which had been left in place, caused his death by eroding through the aorta.", "contents": "Double prosthetic aortic valve. Late aortic erosion by Hufnagel valve. This is a case report of a patient who had one of the first Hufnagel ball-valve prostheses inserted in 1954 for severe aortic incompetence. He obtained relief of symptoms for 14 years before recurrence necessitated the insertion of a Starr-Edwards valve. The patients was symptom-free for another seven years before the original valve, which had been left in place, caused his death by eroding through the aorta.", "PMID": 975986}
{"id": "pubmed23n0032_10522", "title": "Tracheal obstruction associated with a fungus ball. A case of primary tracheal candidiasis.", "content": "An elderly patient developed acute respiratory failure secondary to airway obstruction by a Candida fungus ball which probably developed secondary to the use of broad-spectrum antibiotics when the patient had a decreased gag reflex while in a semicomatose state. The frequency of pulmonary fungus balls and their precipitating causes are briefly discussed. This potentially frequent, previously unreported complication should be recognized as a contributing factor in the development of acute respiratory failure requiring management by bronchoscopic techniques, as opposed to intubation.", "contents": "Tracheal obstruction associated with a fungus ball. A case of primary tracheal candidiasis. An elderly patient developed acute respiratory failure secondary to airway obstruction by a Candida fungus ball which probably developed secondary to the use of broad-spectrum antibiotics when the patient had a decreased gag reflex while in a semicomatose state. The frequency of pulmonary fungus balls and their precipitating causes are briefly discussed. This potentially frequent, previously unreported complication should be recognized as a contributing factor in the development of acute respiratory failure requiring management by bronchoscopic techniques, as opposed to intubation.", "PMID": 975987}
{"id": "pubmed23n0032_10523", "title": "Pneumonitis and pulmonary abscess associated with Moraxella nonliquefaciens.", "content": "A 78-year-old man with pneumonitis and pulmonary abscess associated with Moraxella nonliquefaciens is presented. This organism was found by culture of both transtracheal aspirate and sputum. No previous reports have associated M nonliquefaciens with infection of the lower respiratory tract, although sinusitis and bronchitis have been reported. Possible predisposing factors in our patient included carcinoma of the larynx, as well as alcohol ingestion and cigarette smoking.", "contents": "Pneumonitis and pulmonary abscess associated with Moraxella nonliquefaciens. A 78-year-old man with pneumonitis and pulmonary abscess associated with Moraxella nonliquefaciens is presented. This organism was found by culture of both transtracheal aspirate and sputum. No previous reports have associated M nonliquefaciens with infection of the lower respiratory tract, although sinusitis and bronchitis have been reported. Possible predisposing factors in our patient included carcinoma of the larynx, as well as alcohol ingestion and cigarette smoking.", "PMID": 975988}
{"id": "pubmed23n0032_10524", "title": "Tag-along pacemaker.", "content": "In a R-wave-inhibited pulse generator, an increased interval between pacing artifacts giving an apparently slow rate was not indicative of impending battery failure in two patients. The pacing artifacts appeared as a tag-along phenomenon following a slow idioventricular rhythm as the result of an exit block due to (1) hyperkalemia and (2) perielectrode fibrosis. Simple tests demonstrated a normally functioning R-wave sensing circuit and pacemaker.", "contents": "Tag-along pacemaker. In a R-wave-inhibited pulse generator, an increased interval between pacing artifacts giving an apparently slow rate was not indicative of impending battery failure in two patients. The pacing artifacts appeared as a tag-along phenomenon following a slow idioventricular rhythm as the result of an exit block due to (1) hyperkalemia and (2) perielectrode fibrosis. Simple tests demonstrated a normally functioning R-wave sensing circuit and pacemaker.", "PMID": 975989}
{"id": "pubmed23n0032_10525", "title": "Coccidioidal pericarditis.", "content": "A 26-year-old man developed coccidioidomycosis which resulted in myocarditis associated with congestive heart failure. A pericardial effusion developed and progressed to constrictive pericarditis. A pericardiectomy was performed and revealed that the pericarditis was due to Coccidioides immitis. The patient was subsequently treated with amphotericin B and showed marked improvement.", "contents": "Coccidioidal pericarditis. A 26-year-old man developed coccidioidomycosis which resulted in myocarditis associated with congestive heart failure. A pericardial effusion developed and progressed to constrictive pericarditis. A pericardiectomy was performed and revealed that the pericarditis was due to Coccidioides immitis. The patient was subsequently treated with amphotericin B and showed marked improvement.", "PMID": 975990}
{"id": "pubmed23n0032_10526", "title": "Tonsillar hypertrophy in an adult with obesity-hypoventilation syndrome. The use of the flow-volume loop.", "content": "A 29-year-old obese man had marked tonsillar hypertrophy, somnolence, hypoxemia, and hypercapnia. Endotracheal intubation followed by tracheostomy relieved the hypoventilation. Weight loss improved the arterial blood gas levels. Sequential upright and supine flow-volume loops were compatible with a fixed upper-airway obstruction (such as would occur) with enlarged tonsils) prior to tonsillectomy. Following surgery, the expiratory flow-volume curve was abnormal in the supine position, consistent with the additional diagnosis of posterior pharyngeal hypotonia. Thus, in this patient the unique combination of tonsillar hypertrophy, posterior pharyngeal hypotonia, obesity, and a depressed respiratory center led to retention of carbon dioxide.", "contents": "Tonsillar hypertrophy in an adult with obesity-hypoventilation syndrome. The use of the flow-volume loop. A 29-year-old obese man had marked tonsillar hypertrophy, somnolence, hypoxemia, and hypercapnia. Endotracheal intubation followed by tracheostomy relieved the hypoventilation. Weight loss improved the arterial blood gas levels. Sequential upright and supine flow-volume loops were compatible with a fixed upper-airway obstruction (such as would occur) with enlarged tonsils) prior to tonsillectomy. Following surgery, the expiratory flow-volume curve was abnormal in the supine position, consistent with the additional diagnosis of posterior pharyngeal hypotonia. Thus, in this patient the unique combination of tonsillar hypertrophy, posterior pharyngeal hypotonia, obesity, and a depressed respiratory center led to retention of carbon dioxide.", "PMID": 975991}
{"id": "pubmed23n0032_10527", "title": "Precocious myocardial infarction after radiation treatment for Hodgkin's disease.", "content": "A 26-year-old man received extensive cardiac radiation in the course of treatment of mediastinal Hodgkin's disease, and six years later, he experienced an acute myocardial infarction. Angiographic studies demonstrated extensive atherosclerotic abnormalities in the coronary arterial system. It is suggested that radiation-induced injury was a provocation of these precocious arterial abnormalities.", "contents": "Precocious myocardial infarction after radiation treatment for Hodgkin's disease. A 26-year-old man received extensive cardiac radiation in the course of treatment of mediastinal Hodgkin's disease, and six years later, he experienced an acute myocardial infarction. Angiographic studies demonstrated extensive atherosclerotic abnormalities in the coronary arterial system. It is suggested that radiation-induced injury was a provocation of these precocious arterial abnormalities.", "PMID": 975992}
{"id": "pubmed23n0032_10528", "title": "Unusually high pacemaker threshold in severe myxedema. Decrease with thyroid hormone therapy.", "content": "In a patient with myxedema and complete heart block, an usually high pacemaker threshold was observed initially during transvenous right ventricular endocardial pacing. The pacemaker threshold gradually decreased with thyroid replacement therapy, suggesting that the lack of thyroid hormone in some patients might increase the pacemaker threshold.", "contents": "Unusually high pacemaker threshold in severe myxedema. Decrease with thyroid hormone therapy. In a patient with myxedema and complete heart block, an usually high pacemaker threshold was observed initially during transvenous right ventricular endocardial pacing. The pacemaker threshold gradually decreased with thyroid replacement therapy, suggesting that the lack of thyroid hormone in some patients might increase the pacemaker threshold.", "PMID": 975993}
{"id": "pubmed23n0032_10529", "title": "Retroclavicular route for electrode placement in endocardial pacemakers.", "content": "In order to avoid skin erosion and electrode infection in endocardial pacemakers placed through the external jugular vein, we direct the wires from their point of entry into the vessel to the pacing unit placed in the pectoral region by dissecting a retroclavicular tunnel. This can be done under general or local anesthesia, and so far we have not seen any injuries to the subclavian vein. In this way the entire pathway of the cables is deep enough so that they cannot be palpated through the skin, and the dangers of exposure through erosion are minimized.", "contents": "Retroclavicular route for electrode placement in endocardial pacemakers. In order to avoid skin erosion and electrode infection in endocardial pacemakers placed through the external jugular vein, we direct the wires from their point of entry into the vessel to the pacing unit placed in the pectoral region by dissecting a retroclavicular tunnel. This can be done under general or local anesthesia, and so far we have not seen any injuries to the subclavian vein. In this way the entire pathway of the cables is deep enough so that they cannot be palpated through the skin, and the dangers of exposure through erosion are minimized.", "PMID": 975994}
{"id": "pubmed23n0032_10530", "title": "Geographical variability in the pericentric inversion system of the grasshopper Trimerotropis pseudofasciata.", "content": "Island and mainland populations of Trimerotropis pseudofasciata from California were compared with respect to the nature and extent of their percentric inversion systems. Island populations generally have more chromosomes polymorphic for centromere position than mainland populations and a considerably higher percentage of the genome in these island populations is in a structurally heterozygous state. Thus, although geographically peripheral, the islands provide habitats capable of supporting denser and more chromosomally polymorphic populations than the mainland. Chiasmata are generally localized to terminal positions in all classes of chromosomes and do not occur in the inverted regions of inversion heterozygotes. Chiasma frequency is highest in inversion homozygotes. It is hypothesized that the inversion system in T. pseudofasciata serves the dual synergistic function of preserving allelic sequences in the inversion region intact through inversion heterozygosity and limiting the generation of variability in regions outside the inversion by increasing terminal chiasmata. Additionally, it is argued that it is the gene sequence on only the inversion chromosome that is important in Trimerotropis. This condition contrasts with the \"co-adapted\" pattern seen in Drosophila where the gene sequences on both chromosomes in the inversion heterozygote are simultaneously important.", "contents": "Geographical variability in the pericentric inversion system of the grasshopper Trimerotropis pseudofasciata. Island and mainland populations of Trimerotropis pseudofasciata from California were compared with respect to the nature and extent of their percentric inversion systems. Island populations generally have more chromosomes polymorphic for centromere position than mainland populations and a considerably higher percentage of the genome in these island populations is in a structurally heterozygous state. Thus, although geographically peripheral, the islands provide habitats capable of supporting denser and more chromosomally polymorphic populations than the mainland. Chiasmata are generally localized to terminal positions in all classes of chromosomes and do not occur in the inverted regions of inversion heterozygotes. Chiasma frequency is highest in inversion homozygotes. It is hypothesized that the inversion system in T. pseudofasciata serves the dual synergistic function of preserving allelic sequences in the inversion region intact through inversion heterozygosity and limiting the generation of variability in regions outside the inversion by increasing terminal chiasmata. Additionally, it is argued that it is the gene sequence on only the inversion chromosome that is important in Trimerotropis. This condition contrasts with the \"co-adapted\" pattern seen in Drosophila where the gene sequences on both chromosomes in the inversion heterozygote are simultaneously important.", "PMID": 976009}
{"id": "pubmed23n0032_10531", "title": "Study of mitomycin C-induced chromosomal exchange.", "content": "Mitomycin C-induced sister chromatic exchange and quadriradial formation were studied in chromosomes from Muntiacus muntjac fibroblasts and A9, a transformed mouse cell line. We present the first direct cytological evidence for the induction of true somatic recombination involving homologous chromosomes. Analysis of the quadriradials from Muntjac cells indicates a non-random distribution with respect to the chromosomes involved and with respect to the points of conjunction. Sister chromatid exchange and quadriradial formation may reflect the outcome of repair processes involving a high frequency of homologous exchanges in the interphase nucleus.", "contents": "Study of mitomycin C-induced chromosomal exchange. Mitomycin C-induced sister chromatic exchange and quadriradial formation were studied in chromosomes from Muntiacus muntjac fibroblasts and A9, a transformed mouse cell line. We present the first direct cytological evidence for the induction of true somatic recombination involving homologous chromosomes. Analysis of the quadriradials from Muntjac cells indicates a non-random distribution with respect to the chromosomes involved and with respect to the points of conjunction. Sister chromatid exchange and quadriradial formation may reflect the outcome of repair processes involving a high frequency of homologous exchanges in the interphase nucleus.", "PMID": 976010}
{"id": "pubmed23n0032_10532", "title": "The site of 5S RNA genes in primates. I. The great apes.", "content": "A major site of genes for 5S RNA has been localized in representative members of the family Pongidae by means of hybridization in situ. These genes are shown to be concentrated in the most distal bands of the primate chromosome arm homologous to human chromosome 1q.", "contents": "The site of 5S RNA genes in primates. I. The great apes. A major site of genes for 5S RNA has been localized in representative members of the family Pongidae by means of hybridization in situ. These genes are shown to be concentrated in the most distal bands of the primate chromosome arm homologous to human chromosome 1q.", "PMID": 976011}
{"id": "pubmed23n0032_10533", "title": "Nucleoli and ploidy in Potorous cells (PTK2) in vitro.", "content": "Most cells of the male PTK2 cell line contain one nucleolus, and they are diploid. However, a proportion of cells have more than one nucleolus. Cells with two and three nucleoli were isolated and cloned into viable populations. Greater than 90% of the cells in these clonal populations maintained the abnormal nucleolar number of the originally isolated cell. Karyotype analysis of cells with two and three nucleoli demonstrated that the cells were respectively tetraploid and hexaploid. It was concluded that in PTK2 cells, nucleolarity is a good index of ploidy even if the ploidy level is abnormal. Furthermore, long term monitoring of the tetraploid cells demonstrated virtually no tendency towards reversion to the diploid condition as suggested by other studies in Potorous.", "contents": "Nucleoli and ploidy in Potorous cells (PTK2) in vitro. Most cells of the male PTK2 cell line contain one nucleolus, and they are diploid. However, a proportion of cells have more than one nucleolus. Cells with two and three nucleoli were isolated and cloned into viable populations. Greater than 90% of the cells in these clonal populations maintained the abnormal nucleolar number of the originally isolated cell. Karyotype analysis of cells with two and three nucleoli demonstrated that the cells were respectively tetraploid and hexaploid. It was concluded that in PTK2 cells, nucleolarity is a good index of ploidy even if the ploidy level is abnormal. Furthermore, long term monitoring of the tetraploid cells demonstrated virtually no tendency towards reversion to the diploid condition as suggested by other studies in Potorous.", "PMID": 976012}
{"id": "pubmed23n0032_10534", "title": "Configurational changes in chromatids from helical to banded structures.", "content": "Induction of configurational changes in the helical chromatids of air dried chromosomes was used to explore the mechanism of G-banding. From the water-Giemsa stained metaphase spreads of Chinese hamster cells, chromosomes having clearly helical chromatids were selected and photographed. Then the chromosomes were decolorized, treated with trypsin, and restained with saline-Giemsa (1X SSC). Such procedures were repeatedly carried out upon the same chromosomes. Subsequent examination of the chromosomes showed that configurational changes from a helical structure to a banded structure had occurred. Some chromosomes revealed a variety of transitional changes between these two configurations. During the repeated G-banding treatments, the distances between bands along the same chromatids changed each time. The results obtained seem to indicate that the G-banding results from locally induced compaction of chromosomal materials along the chromatids.", "contents": "Configurational changes in chromatids from helical to banded structures. Induction of configurational changes in the helical chromatids of air dried chromosomes was used to explore the mechanism of G-banding. From the water-Giemsa stained metaphase spreads of Chinese hamster cells, chromosomes having clearly helical chromatids were selected and photographed. Then the chromosomes were decolorized, treated with trypsin, and restained with saline-Giemsa (1X SSC). Such procedures were repeatedly carried out upon the same chromosomes. Subsequent examination of the chromosomes showed that configurational changes from a helical structure to a banded structure had occurred. Some chromosomes revealed a variety of transitional changes between these two configurations. During the repeated G-banding treatments, the distances between bands along the same chromatids changed each time. The results obtained seem to indicate that the G-banding results from locally induced compaction of chromosomal materials along the chromatids.", "PMID": 976013}
{"id": "pubmed23n0032_10535", "title": "The organization of repeated DNA sequences in the human genome.", "content": "The arrangement of repetitive and non-repetitive DNA sequences was studied in the human genome. By Ag+-Cs2SO4 density gradient centrifugations of human DNA at different fragment size reannealed to different Cot values and c-RNA hybridization experiments, we have shown the presence of two repetitive DNA fractions, called fast and slow intermediate DNA, with different pattern of sequence organization. The fast intermediate DNA sequences (6% of the genome; CsCl density in renatured form: 1.703 g/ml) are in part clustered in fragments greater than 24,000 nucleotide pairs and in part in fragments ranging from 1,800 to 600 nucleotide pairs spaced with longer more complex sequences. The slow intermediate DNA sequences (30% of the genome; CsCl density in renatured form: 1.707 g/ml) appear to be finely interspersed with non-repetitive sequences. At a DNA fragment size of 600 nucleotide pairs only a third of the slow intermediate DNA sequences are free of unique sequences, while the other two thirds are still organized with unique sequences. It has also been shown that a great amount of the repetitive DNA sequence transcripts in heterogeneous nuclear RNA of HeLa cells are complementary to slow intermediate DNA sequences.", "contents": "The organization of repeated DNA sequences in the human genome. The arrangement of repetitive and non-repetitive DNA sequences was studied in the human genome. By Ag+-Cs2SO4 density gradient centrifugations of human DNA at different fragment size reannealed to different Cot values and c-RNA hybridization experiments, we have shown the presence of two repetitive DNA fractions, called fast and slow intermediate DNA, with different pattern of sequence organization. The fast intermediate DNA sequences (6% of the genome; CsCl density in renatured form: 1.703 g/ml) are in part clustered in fragments greater than 24,000 nucleotide pairs and in part in fragments ranging from 1,800 to 600 nucleotide pairs spaced with longer more complex sequences. The slow intermediate DNA sequences (30% of the genome; CsCl density in renatured form: 1.707 g/ml) appear to be finely interspersed with non-repetitive sequences. At a DNA fragment size of 600 nucleotide pairs only a third of the slow intermediate DNA sequences are free of unique sequences, while the other two thirds are still organized with unique sequences. It has also been shown that a great amount of the repetitive DNA sequence transcripts in heterogeneous nuclear RNA of HeLa cells are complementary to slow intermediate DNA sequences.", "PMID": 976014}
{"id": "pubmed23n0032_10536", "title": "In vitro transcription by isolated nuclei of Rhynchosciara americana salivary glands. Characteristics of incorporation and inhibition by alpha-amanitin.", "content": "A method for the isolation of polytene nuclei from salivary glands cells of the Diptera Rhynchosciara americana is described. The stage-specific morphological pattern of the chromosome is maintained during the isolation. The isolated nuclei show two distinct RNA polymerase activities, namely I and II, characterized on the basis of ionic requirements and alpha-amanitin sensitivity. Studies of the product under the incubation conditions show that the system allows the synthesis of high-molecular weight RNA, beside a low molecular weight peak which may comprise pre-4S and 5S RNAs. Autoradiographic studies carried out in the presence or absence of the toxin alpha-amanitin showed that micronucleoli contain products of RNA polymerase type I activity (ribosomal RNA) and that the DNA puffs are engaged in alpha-amanitin sensitive RNA synthesis and thus are sites of polymerase type II activity.", "contents": "In vitro transcription by isolated nuclei of Rhynchosciara americana salivary glands. Characteristics of incorporation and inhibition by alpha-amanitin. A method for the isolation of polytene nuclei from salivary glands cells of the Diptera Rhynchosciara americana is described. The stage-specific morphological pattern of the chromosome is maintained during the isolation. The isolated nuclei show two distinct RNA polymerase activities, namely I and II, characterized on the basis of ionic requirements and alpha-amanitin sensitivity. Studies of the product under the incubation conditions show that the system allows the synthesis of high-molecular weight RNA, beside a low molecular weight peak which may comprise pre-4S and 5S RNAs. Autoradiographic studies carried out in the presence or absence of the toxin alpha-amanitin showed that micronucleoli contain products of RNA polymerase type I activity (ribosomal RNA) and that the DNA puffs are engaged in alpha-amanitin sensitive RNA synthesis and thus are sites of polymerase type II activity.", "PMID": 976015}
{"id": "pubmed23n0032_10537", "title": "Sister chromatid differentiation and exchanges in adult mudminnows (Umbra limi) after in vivo exposure to 5-bromodeoxyuridine.", "content": "An in vivo system for the detection of sister chromatid exchange (SCE) in the central mudminnow, Umbra limi, is presented. Sister chromatid differential (SCD) and SCE were demonstrated by fluorescent and Giemsa procedures 5 to 6 days after the fish were injected with 500 mug/g of BrdU. The exchange rate was found to be 2.64 SCEs metaphase in the intestines and 2.42 SCEs/metaphase in the gills. SCE analysis in U. limi should be a useful tool for measuring the mutagenicity of water-borne chemicals.", "contents": "Sister chromatid differentiation and exchanges in adult mudminnows (Umbra limi) after in vivo exposure to 5-bromodeoxyuridine. An in vivo system for the detection of sister chromatid exchange (SCE) in the central mudminnow, Umbra limi, is presented. Sister chromatid differential (SCD) and SCE were demonstrated by fluorescent and Giemsa procedures 5 to 6 days after the fish were injected with 500 mug/g of BrdU. The exchange rate was found to be 2.64 SCEs metaphase in the intestines and 2.42 SCEs/metaphase in the gills. SCE analysis in U. limi should be a useful tool for measuring the mutagenicity of water-borne chemicals.", "PMID": 976016}
{"id": "pubmed23n0032_10538", "title": "Satellite DNA in the kangaroo Macropus rufogriseus.", "content": "Two distinct satellite DNAs, amounting to 25% of the total DNA, were isolated from the nuclei of the red-necked wallaby, Macropus rufogriseus. The physical properties of native, single-stranded and reassociated molecules were studied in buoyant-density gradient centrifugation. The homogeneity of each satellite fraction was examined using melting characteristics of native and reassociated DNA, and renaturation kinetics. These data suggest that sequence heterogeneity exists in both fractions. Each satellite fraction was found by in situ hybridization to be localized in heterochromatin of interphase nuclei and in the centromeric regions of metaphase chromosomes. The chromosomal distributions of the two satellite DNAs differentiate the sex chromosomes, which have sequences of only one satellite, from the autosomes which have sequences of both satellites in the centromeric heterochromatin. Giemsa C-banding techniques also showed a differentiation of the centromeric regions of sex chromosomes from those of the autosomes.", "contents": "Satellite DNA in the kangaroo Macropus rufogriseus. Two distinct satellite DNAs, amounting to 25% of the total DNA, were isolated from the nuclei of the red-necked wallaby, Macropus rufogriseus. The physical properties of native, single-stranded and reassociated molecules were studied in buoyant-density gradient centrifugation. The homogeneity of each satellite fraction was examined using melting characteristics of native and reassociated DNA, and renaturation kinetics. These data suggest that sequence heterogeneity exists in both fractions. Each satellite fraction was found by in situ hybridization to be localized in heterochromatin of interphase nuclei and in the centromeric regions of metaphase chromosomes. The chromosomal distributions of the two satellite DNAs differentiate the sex chromosomes, which have sequences of only one satellite, from the autosomes which have sequences of both satellites in the centromeric heterochromatin. Giemsa C-banding techniques also showed a differentiation of the centromeric regions of sex chromosomes from those of the autosomes.", "PMID": 976017}
{"id": "pubmed23n0032_10539", "title": "Karyotype differences in the crenaticeps-group of Atractomorpha (Orthoptera, Acridoidea, Pyrgomorphidae).", "content": "The four known species of the crenaticeps-group of the genus Atractomorpha have 2n (male)=18+X0. All members of the complement are rod-chromosomes and the smallest autosome (no. 9) is megameric. The four species have similar amounts of euchromatin but differ markedly in the amount of heterochromatin present in their genomes. In A. similis, A. crenaticeps and the unnamed species, \"Species-1\", there are distinct proximal segments of heterochromatin in the eight large autosomes. In A. similis these chromosomes also have prominent distal segments of heterochromatin. The fourth species, A. australis, has no visible heterochromatin in its eight large autosomes except for a small segment at the proximal end of autosome 4. In all four species, the heterochromatic segments influence chiasma frequency and chiasma position. Moreover the overall chiasma frequency is lowest in A. similis with most heterochromatin and highest in A. australis with least heterochromatin.", "contents": "Karyotype differences in the crenaticeps-group of Atractomorpha (Orthoptera, Acridoidea, Pyrgomorphidae). The four known species of the crenaticeps-group of the genus Atractomorpha have 2n (male)=18+X0. All members of the complement are rod-chromosomes and the smallest autosome (no. 9) is megameric. The four species have similar amounts of euchromatin but differ markedly in the amount of heterochromatin present in their genomes. In A. similis, A. crenaticeps and the unnamed species, \"Species-1\", there are distinct proximal segments of heterochromatin in the eight large autosomes. In A. similis these chromosomes also have prominent distal segments of heterochromatin. The fourth species, A. australis, has no visible heterochromatin in its eight large autosomes except for a small segment at the proximal end of autosome 4. In all four species, the heterochromatic segments influence chiasma frequency and chiasma position. Moreover the overall chiasma frequency is lowest in A. similis with most heterochromatin and highest in A. australis with least heterochromatin.", "PMID": 976018}
{"id": "pubmed23n0032_10540", "title": "Telomeric satellite DNA functions in regulating recombination.", "content": "Molecular and cytogenetical analyses of three sibling species of Australian grasshopper, Atractomorpha australis, A. species-1 and A. similis, resolves one of the long standing problems of highly repeated DNA. In this system satellite DNA functions in regulating the level and position of recombination, irrespective of whether the repeated DNA is located in telomeric or centric regions. Even though the three species do not differ in their euchromatic genome sizes, their relative DNA contents are 1.00/1.10/1.41, the difference in genome size being due solely to visible centric or telomeric blocks of heterochromatin. Antibiotic analytical and preparative ultracentrifugation, in situ hybridization and renaturation kinetic analyses reveal that a large cryptic satellite of A. similis constitutes the heterochromatic telomeric blocks of nearly all autosomes and that the DNA of this satellite is highly repeated. Comparison of these grasshopper data with published literature of heterochromatic rearrangements in Drosophila and with heterochromatin distribution and recombination patterns in diploid plant species reveals that in every case heterochromatin is implicated in some form of alteration in the meiotic recombination system.", "contents": "Telomeric satellite DNA functions in regulating recombination. Molecular and cytogenetical analyses of three sibling species of Australian grasshopper, Atractomorpha australis, A. species-1 and A. similis, resolves one of the long standing problems of highly repeated DNA. In this system satellite DNA functions in regulating the level and position of recombination, irrespective of whether the repeated DNA is located in telomeric or centric regions. Even though the three species do not differ in their euchromatic genome sizes, their relative DNA contents are 1.00/1.10/1.41, the difference in genome size being due solely to visible centric or telomeric blocks of heterochromatin. Antibiotic analytical and preparative ultracentrifugation, in situ hybridization and renaturation kinetic analyses reveal that a large cryptic satellite of A. similis constitutes the heterochromatic telomeric blocks of nearly all autosomes and that the DNA of this satellite is highly repeated. Comparison of these grasshopper data with published literature of heterochromatic rearrangements in Drosophila and with heterochromatin distribution and recombination patterns in diploid plant species reveals that in every case heterochromatin is implicated in some form of alteration in the meiotic recombination system.", "PMID": 976019}
{"id": "pubmed23n0032_10541", "title": "Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). II. Variation in the pattern of C-banding.", "content": "The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromosomal rearrangements. The \"Ancestral\" race has a telocentric chromosome complements with large procentric C-bands which are structurally double on six pairs of chromosomes. The centromeres are unstained. The \"General Purpose\" race has a C-banding pattern very similar to that seen in other Acridine grasshoppers with the majority of its chromosomes showing a centromeric localisation of the bands. The two southern races, which show a complex polymorphism for presumed pericentric inversions on all twelve chromosomes, also show an unusually high level of interstitial and terminal C-bands. The different locations and numbers of these bands allow unambiguous identification of all the chromosome pairs within the complement. In two cases, there is good evidence to indicate that a C-band redistribution between acrocentric and metacentric chromosomes has occurred by pericentric inversion. Furthermore, C-band variation on the long arm of the metacentric X-chromosome indicates the presence of a large paracentric inversion. This double inversion system has involved over 95% of the X-chromosome. The interstitial and terminal C-bands probably have not resulted from heterochromatin movement within the complement but, more likely, have arisen by saltatory duplication of pre-existing sequences on the chromosome. A new nomenclature system for banded chromosomes is proposed which allows most kinds of chromosomal restructuring and rearrangement to be adequately enumerated.", "contents": "Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). II. Variation in the pattern of C-banding. The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromosomal rearrangements. The \"Ancestral\" race has a telocentric chromosome complements with large procentric C-bands which are structurally double on six pairs of chromosomes. The centromeres are unstained. The \"General Purpose\" race has a C-banding pattern very similar to that seen in other Acridine grasshoppers with the majority of its chromosomes showing a centromeric localisation of the bands. The two southern races, which show a complex polymorphism for presumed pericentric inversions on all twelve chromosomes, also show an unusually high level of interstitial and terminal C-bands. The different locations and numbers of these bands allow unambiguous identification of all the chromosome pairs within the complement. In two cases, there is good evidence to indicate that a C-band redistribution between acrocentric and metacentric chromosomes has occurred by pericentric inversion. Furthermore, C-band variation on the long arm of the metacentric X-chromosome indicates the presence of a large paracentric inversion. This double inversion system has involved over 95% of the X-chromosome. The interstitial and terminal C-bands probably have not resulted from heterochromatin movement within the complement but, more likely, have arisen by saltatory duplication of pre-existing sequences on the chromosome. A new nomenclature system for banded chromosomes is proposed which allows most kinds of chromosomal restructuring and rearrangement to be adequately enumerated.", "PMID": 976020}
{"id": "pubmed23n0032_10542", "title": "Outline of chronohygiene.", "content": "Since chronotherapy already manipulates rhythmical functions, it is the important task of chronohygiene to outline the object of therapy i.e. health, from chronobiological points of view. Only on such a basis can chronohygiene be extended to the preventive provisions of health care and health education in regard to the biological time structure. Health can be characterized as an optimal state of harmonious time structure and its integration into the temporal orders of the environment. The circannual variations of an organism establish a seasonal risk cycle changing the inner preconditions of therapy as well. Moreover, chronohygiene must raise the question of whether the biological circannual rhythm is an integrating component of the human time organization, or if it may be levelled by civilization. Circadian variations of the organism not only modify the preconditions of therapy, but in particular those of activity and efficiency. The nocturnal maximum of functional economy normally is protected against exploitation by a simultaneous minimum of vigilance, which provides the nightly recovery. Therefore the destruction of this phase relationship by night-work must worsen recovery conditions. The disturbance of the time structure in the organism by night-work not only affects the circadian system, but faster rhythms as well. The phase position of the circadian rhythms is closely related to the individual responsiveness of the autonomous system as well as to the pulse-respiration-ratio. The latter can serve as a chronobiological criterion for evaluating suitability or risk of night- and shift-work. The effort of chronohygiene is based on the fact that disturbances of the time structure represent just a complementary aspect to disturbances of the spatial structure of the organism.", "contents": "Outline of chronohygiene. Since chronotherapy already manipulates rhythmical functions, it is the important task of chronohygiene to outline the object of therapy i.e. health, from chronobiological points of view. Only on such a basis can chronohygiene be extended to the preventive provisions of health care and health education in regard to the biological time structure. Health can be characterized as an optimal state of harmonious time structure and its integration into the temporal orders of the environment. The circannual variations of an organism establish a seasonal risk cycle changing the inner preconditions of therapy as well. Moreover, chronohygiene must raise the question of whether the biological circannual rhythm is an integrating component of the human time organization, or if it may be levelled by civilization. Circadian variations of the organism not only modify the preconditions of therapy, but in particular those of activity and efficiency. The nocturnal maximum of functional economy normally is protected against exploitation by a simultaneous minimum of vigilance, which provides the nightly recovery. Therefore the destruction of this phase relationship by night-work must worsen recovery conditions. The disturbance of the time structure in the organism by night-work not only affects the circadian system, but faster rhythms as well. The phase position of the circadian rhythms is closely related to the individual responsiveness of the autonomous system as well as to the pulse-respiration-ratio. The latter can serve as a chronobiological criterion for evaluating suitability or risk of night- and shift-work. The effort of chronohygiene is based on the fact that disturbances of the time structure represent just a complementary aspect to disturbances of the spatial structure of the organism.", "PMID": 976021}
{"id": "pubmed23n0032_10543", "title": "Circadian periodic response of Phaseolus vulgaris l. to 2,4-dichlorophenoxyacetic acid.", "content": "The susceptibility of bean plants, Phaseolus vulgaris L., cv. Executive, to 2,4-dichlorophentoxyacetic acid (2,4-D) appeared to depend upon the time of application. Oscillations in response to 2,4-D were evident in plants subjected to conditions of alternating light and dark spans, continuous illumination or darkness. The fresh and dry weight of plant material was generally less when 2,4-D was applied to plants near the later portions of the light span.", "contents": "Circadian periodic response of Phaseolus vulgaris l. to 2,4-dichlorophenoxyacetic acid. The susceptibility of bean plants, Phaseolus vulgaris L., cv. Executive, to 2,4-dichlorophentoxyacetic acid (2,4-D) appeared to depend upon the time of application. Oscillations in response to 2,4-D were evident in plants subjected to conditions of alternating light and dark spans, continuous illumination or darkness. The fresh and dry weight of plant material was generally less when 2,4-D was applied to plants near the later portions of the light span.", "PMID": 976022}
{"id": "pubmed23n0032_10544", "title": "Advances in human chronopharmacology.", "content": "Regular and thus predictable changes in biologic susceptibility and response to a large variety of physical as well as chemical agents can now be viewed as rather common phenomena. Chronopharmacology involves both the investigation of drug effects as a function of biologic timing and the investigation of drug effects upon rhythm characteristics (the period, tau, the acrophase, phi, the amplitude, A, and the mesor, M). Illustrative examples of circadian (tau congruent to 24 hrs) chronopharmacology in man are summarized in up-dated tables, keeping in mind that the objective demonstration of chronopharmacologic facts demands the use of an appropriate methodology. Circadian changes in the effects of various chemical agents have been documented: histamine, sodium salicylate, acetylcholine, halothane, prostaglandin F2alpha, reserpine, cyproheptadine, ethanol, insulin, chlorothiazide, oxymetholone, orcinprenaline and SCH 1000 (the latter being bronchodilators), Indomethacin, lignocaine, ACTH, cortisol and various synthetic corticosteroids. Despite that pharmacological facts are well documented in man for circadian rhythms, studies are not only restricted to the 24-h period but have been already extended to rhythms of periods of e.g. approximately 30 days as well as approximately 1 year. For a better understanding of chronopharmacologic findings, three new concepts must be considered: a) the chronokinetics of a drug, defined as both rhythmic (circadian) changes in the drug bioavailability (and/or pharmacokinetic effects) and its excretion (urinary, among others); b) the chronesthesy of a biosystem to a drug, or the circadian change in the susceptibility of any biosystem to a drug (including organ systems, tumors, parasites, etc.); and c) The chronergy of a drug, or the rhythmic change in the overall effects and the effectiveness of a drug. This term takes into consideration the chronokinetics and the chronesthesies of the organismic biosystems involved. Presently, one of the aims of chronopharmacology is to solve problems of drug optimization, i.e. to enhance the desired efficiency of a corticosteroid or other medications, while reducing undesired effects. In the human organism, among other animal species, the metabolic fate of a pharmacologic agent (as well as that of a nutrient) is not constant as a function of biological time. The metabolic pathways are neither open permanently, nor open with a constant patency along the 24-h scale, among other bioperiodic domains. Thus, the chronobiologic approach concerning pharmacologic phenomena involves a lesser risk of errors and/or false information than the conventional homeostatic approach.", "contents": "Advances in human chronopharmacology. Regular and thus predictable changes in biologic susceptibility and response to a large variety of physical as well as chemical agents can now be viewed as rather common phenomena. Chronopharmacology involves both the investigation of drug effects as a function of biologic timing and the investigation of drug effects upon rhythm characteristics (the period, tau, the acrophase, phi, the amplitude, A, and the mesor, M). Illustrative examples of circadian (tau congruent to 24 hrs) chronopharmacology in man are summarized in up-dated tables, keeping in mind that the objective demonstration of chronopharmacologic facts demands the use of an appropriate methodology. Circadian changes in the effects of various chemical agents have been documented: histamine, sodium salicylate, acetylcholine, halothane, prostaglandin F2alpha, reserpine, cyproheptadine, ethanol, insulin, chlorothiazide, oxymetholone, orcinprenaline and SCH 1000 (the latter being bronchodilators), Indomethacin, lignocaine, ACTH, cortisol and various synthetic corticosteroids. Despite that pharmacological facts are well documented in man for circadian rhythms, studies are not only restricted to the 24-h period but have been already extended to rhythms of periods of e.g. approximately 30 days as well as approximately 1 year. For a better understanding of chronopharmacologic findings, three new concepts must be considered: a) the chronokinetics of a drug, defined as both rhythmic (circadian) changes in the drug bioavailability (and/or pharmacokinetic effects) and its excretion (urinary, among others); b) the chronesthesy of a biosystem to a drug, or the circadian change in the susceptibility of any biosystem to a drug (including organ systems, tumors, parasites, etc.); and c) The chronergy of a drug, or the rhythmic change in the overall effects and the effectiveness of a drug. This term takes into consideration the chronokinetics and the chronesthesies of the organismic biosystems involved. Presently, one of the aims of chronopharmacology is to solve problems of drug optimization, i.e. to enhance the desired efficiency of a corticosteroid or other medications, while reducing undesired effects. In the human organism, among other animal species, the metabolic fate of a pharmacologic agent (as well as that of a nutrient) is not constant as a function of biological time. The metabolic pathways are neither open permanently, nor open with a constant patency along the 24-h scale, among other bioperiodic domains. Thus, the chronobiologic approach concerning pharmacologic phenomena involves a lesser risk of errors and/or false information than the conventional homeostatic approach.", "PMID": 976023}
{"id": "pubmed23n0032_10545", "title": "Stromal atypia in anal papillae.", "content": "This report describes the occurrence of stromal atypia in anal papillae. The atypia occurred in two of 15 papillae studied and was present in the larger ones. The stromal atypia is similar to that described to occur in vaginal polyps and in entities such as pseudosarcomatous fasciitis, atypical fibroxanthomas of the skin, radiation dermatitis, and giant-cell cystitis. The atypia is considered to be reactive or reparative.", "contents": "Stromal atypia in anal papillae. This report describes the occurrence of stromal atypia in anal papillae. The atypia occurred in two of 15 papillae studied and was present in the larger ones. The stromal atypia is similar to that described to occur in vaginal polyps and in entities such as pseudosarcomatous fasciitis, atypical fibroxanthomas of the skin, radiation dermatitis, and giant-cell cystitis. The atypia is considered to be reactive or reparative.", "PMID": 976028}
{"id": "pubmed23n0032_10546", "title": "Adenocarcinoma of the appendix: an unusual case and review.", "content": "Primary adenocarcinoma of the appendix is the rarest form of malignant tumor of the appendiceal stump is described. As with other malignancies, surgical treatment is described. As with other malignancies, surgical treatment is most effective during the early stages of disease. In our series most of the patients who had metastatic disease had fairly rapid downhill courses. The choice of right hemicolectomy with node dissection in all cases is suported.", "contents": "Adenocarcinoma of the appendix: an unusual case and review. Primary adenocarcinoma of the appendix is the rarest form of malignant tumor of the appendiceal stump is described. As with other malignancies, surgical treatment is described. As with other malignancies, surgical treatment is most effective during the early stages of disease. In our series most of the patients who had metastatic disease had fairly rapid downhill courses. The choice of right hemicolectomy with node dissection in all cases is suported.", "PMID": 976029}
{"id": "pubmed23n0032_10547", "title": "Fibroma of the cecum: report of a case.", "content": "A woman suffering from recurrent attacks of abdominal pain was found at operation to have a fibroma of the cecum. The clinical, operative and pathologic findings are described. The medical literature was reviewed and the lesion was found to be uncommon. The importance of specific histologic stains to distinguish the true fibroma from other spindle-cell tumors is stressed.", "contents": "Fibroma of the cecum: report of a case. A woman suffering from recurrent attacks of abdominal pain was found at operation to have a fibroma of the cecum. The clinical, operative and pathologic findings are described. The medical literature was reviewed and the lesion was found to be uncommon. The importance of specific histologic stains to distinguish the true fibroma from other spindle-cell tumors is stressed.", "PMID": 976030}
{"id": "pubmed23n0032_10548", "title": "Elective resection for recurrent sigmoidal volvulus: report of a case.", "content": "A patient with recurrent sigmoidal volvulus who was treated by elective sigmoidal resection is reported. The symptoms and radiologic findings are sufficient for a confident diagnosis to be made before the onset of a major obstructive epidose. The value of barium-enema examination even in the quiescent phase is emphasized. Definitive treatment by sigmoidal resection in the absence of obstruction is safe and should be undertaken as an elective measure. Awareness of the disease and its occurrence in young patients will aid its recognition and reduce mortality from intestinal obstruction.", "contents": "Elective resection for recurrent sigmoidal volvulus: report of a case. A patient with recurrent sigmoidal volvulus who was treated by elective sigmoidal resection is reported. The symptoms and radiologic findings are sufficient for a confident diagnosis to be made before the onset of a major obstructive epidose. The value of barium-enema examination even in the quiescent phase is emphasized. Definitive treatment by sigmoidal resection in the absence of obstruction is safe and should be undertaken as an elective measure. Awareness of the disease and its occurrence in young patients will aid its recognition and reduce mortality from intestinal obstruction.", "PMID": 976031}
{"id": "pubmed23n0032_10549", "title": "Retrorectal herniation of the sigmoid colon: report of a case.", "content": "A rare retrorectal hernia of the sigmoid colon associated with diverticulitis and a presacral abscess is reported. The anatomy of the retrorectal space is reviewed. The value of routine lateral fluoroscopy and overhead films is emphasized.", "contents": "Retrorectal herniation of the sigmoid colon: report of a case. A rare retrorectal hernia of the sigmoid colon associated with diverticulitis and a presacral abscess is reported. The anatomy of the retrorectal space is reviewed. The value of routine lateral fluoroscopy and overhead films is emphasized.", "PMID": 976032}
{"id": "pubmed23n0032_10550", "title": "Sliding colonic Maydl's hernia: report of a case.", "content": "An unusual variety of Maydl's hernia (hernia-in-W) in which all the herniated loops were colon is reported. The unusual anatomy of the hernia and its variations are described. It is important to examine the intraperitoneal intestine between the incarcerated loops in an effort to avoid leaving a nonviable segment of intraperitoneal intestine after repair of the hernia.", "contents": "Sliding colonic Maydl's hernia: report of a case. An unusual variety of Maydl's hernia (hernia-in-W) in which all the herniated loops were colon is reported. The unusual anatomy of the hernia and its variations are described. It is important to examine the intraperitoneal intestine between the incarcerated loops in an effort to avoid leaving a nonviable segment of intraperitoneal intestine after repair of the hernia.", "PMID": 976033}
{"id": "pubmed23n0032_10551", "title": "[Oesophageal function in patiensts with angina (author's transl)].", "content": "Oesophageal functions were measured in 18 patients with angina, 13 healthy volunteers and 29 age-matched patients with reflux disease. Acid clearance was as abnormal in angina patients (88 +/- 46 swallows) as in those with reflux disease (97 +/- 45). The pressure in the lower oesophageal sphincter was low in patients with angina (13 +/- 8 mm Hg). The incidence of hiatal hernia, subjective symptoms during acidification of the oesophagus and results of the reflux provocation test were similar in patients with angina pectoris and healthy controls.", "contents": "[Oesophageal function in patiensts with angina (author's transl)]. Oesophageal functions were measured in 18 patients with angina, 13 healthy volunteers and 29 age-matched patients with reflux disease. Acid clearance was as abnormal in angina patients (88 +/- 46 swallows) as in those with reflux disease (97 +/- 45). The pressure in the lower oesophageal sphincter was low in patients with angina (13 +/- 8 mm Hg). The incidence of hiatal hernia, subjective symptoms during acidification of the oesophagus and results of the reflux provocation test were similar in patients with angina pectoris and healthy controls.", "PMID": 976099}
{"id": "pubmed23n0032_10552", "title": "[Atrial fibrillation and flutter as a complication of acute myocardial infarction (author's transl)].", "content": "Among 909 patients with acute myocardial inarction treated in an intensive care unit between 1970 and 1974, atrial flutter and (or) fibrillation occurred in 124 (13.6%). The incidence of these arrhythmias rose with increasing age and predominantly in paroxysmal form (78%). The clinic mortality of patients with arrhythmias was 42%, while in the remaining 785 it was only 26% (P less than 0.001). Patients with atrial fibrillation and (or) flutter had a higher mean age, more frequently cardiac failure (P less than 0.001) - especially in the prognostically unfavourable severe forms with pulmonary oedema (P less than 0.05) and combined right and left heart failure (P less than 0.001) - and other disorders of impulse conduction or formation and chronic arterial hypertension (P less than 0.01).", "contents": "[Atrial fibrillation and flutter as a complication of acute myocardial infarction (author's transl)]. Among 909 patients with acute myocardial inarction treated in an intensive care unit between 1970 and 1974, atrial flutter and (or) fibrillation occurred in 124 (13.6%). The incidence of these arrhythmias rose with increasing age and predominantly in paroxysmal form (78%). The clinic mortality of patients with arrhythmias was 42%, while in the remaining 785 it was only 26% (P less than 0.001). Patients with atrial fibrillation and (or) flutter had a higher mean age, more frequently cardiac failure (P less than 0.001) - especially in the prognostically unfavourable severe forms with pulmonary oedema (P less than 0.05) and combined right and left heart failure (P less than 0.001) - and other disorders of impulse conduction or formation and chronic arterial hypertension (P less than 0.01).", "PMID": 976100}
{"id": "pubmed23n0032_10553", "title": "[Penicillamine-induced myasthenia in chronic rheumatoid arthritis (author's transl)].", "content": "A myasthenic reaction developed after an average latency of seven to eight months in 12 female patients (average age 51 years) treated with penicillamine for chronic rheumatoid arthritis. In most of them only the purely ocular form was noted, but generalisation of various degrees also occurred. When penicillamine had been discontinued the myasthenic signs were almost always fully reversible, recurring once again when treatment with penicillamine was started. In some instances a transitory symptomatic treatment with cholinesterase inhibitors was necessary. The occurrence of the myasthenic signs in these circumstances is probably similar to the anti-basal membrane nephropathy induced by penicillamine.", "contents": "[Penicillamine-induced myasthenia in chronic rheumatoid arthritis (author's transl)]. A myasthenic reaction developed after an average latency of seven to eight months in 12 female patients (average age 51 years) treated with penicillamine for chronic rheumatoid arthritis. In most of them only the purely ocular form was noted, but generalisation of various degrees also occurred. When penicillamine had been discontinued the myasthenic signs were almost always fully reversible, recurring once again when treatment with penicillamine was started. In some instances a transitory symptomatic treatment with cholinesterase inhibitors was necessary. The occurrence of the myasthenic signs in these circumstances is probably similar to the anti-basal membrane nephropathy induced by penicillamine.", "PMID": 976101}
{"id": "pubmed23n0032_10554", "title": "[Unusual course of traumatic intracranial bleedings (author's transl)].", "content": "Intracranial bleedings occurred in three patients as complications of h-ad trauma, without any initial acute disturbance of consciousness. Only careful observation, which revealed slow deterioration in consciousness, made it possible to establish the diagnosis of intracranial haemorrhage, confirmed angiographically in all three instances.", "contents": "[Unusual course of traumatic intracranial bleedings (author's transl)]. Intracranial bleedings occurred in three patients as complications of h-ad trauma, without any initial acute disturbance of consciousness. Only careful observation, which revealed slow deterioration in consciousness, made it possible to establish the diagnosis of intracranial haemorrhage, confirmed angiographically in all three instances.", "PMID": 976102}
{"id": "pubmed23n0032_10555", "title": "[Results of surgical treatment of early carcinoma of the stomach (author's transl)].", "content": "From 1961 to 1975, 53 patients were operated on for early carcinoma of the stomach, 24 of the total of 57 cancer sites being limited to the mucosa, 33 having already infiltrated into the submucosa. Macroscopically, most of them were ulcerative carcinoma, while histologically the intestinal type predominated. The cumulative five-year survival rate (excluding hospital deaths) was 86.5%, while after \"typical\" carcinoma resection by the Billroth II procedure with removal of the greater and lesser omentum it was 100% for mucosal and 93% for submucosal carcinoma.", "contents": "[Results of surgical treatment of early carcinoma of the stomach (author's transl)]. From 1961 to 1975, 53 patients were operated on for early carcinoma of the stomach, 24 of the total of 57 cancer sites being limited to the mucosa, 33 having already infiltrated into the submucosa. Macroscopically, most of them were ulcerative carcinoma, while histologically the intestinal type predominated. The cumulative five-year survival rate (excluding hospital deaths) was 86.5%, while after \"typical\" carcinoma resection by the Billroth II procedure with removal of the greater and lesser omentum it was 100% for mucosal and 93% for submucosal carcinoma.", "PMID": 976110}
{"id": "pubmed23n0032_10556", "title": "[Cytological classification of bronchial carcinoma (author's transl)].", "content": "Reliability of cytological classification of tumours from material obtained from sputum or bronchial secretion was tested on 116 histologically confirmed cases of bronchial carcinoma. Squamous-cell carcinoma and small-cell undifferentiated carcinoma were most often correctly classified, results being less good with large-cell undifferentiated carcinoma, adenocarcinoma taking an intermediary position.", "contents": "[Cytological classification of bronchial carcinoma (author's transl)]. Reliability of cytological classification of tumours from material obtained from sputum or bronchial secretion was tested on 116 histologically confirmed cases of bronchial carcinoma. Squamous-cell carcinoma and small-cell undifferentiated carcinoma were most often correctly classified, results being less good with large-cell undifferentiated carcinoma, adenocarcinoma taking an intermediary position.", "PMID": 976111}
{"id": "pubmed23n0032_10557", "title": "[Myocardial infarction after accidents (author's transl)].", "content": "In a 27-year-old man blunt chest-wall trauma after a car accident gave rise to several retrosternal pain. Coronary angiography demonstrated severe generalised coronary arteriosclerosis. The history revealed heavy smoking (60 cigarettes daily for ten years). Although it must be assumed that there was severe generalised coronary arteriosclerosis without angina pectoris before the accident, the infarction was considered to be a direct consequence of it: it prematurely precipitated the infarction. In a second case, of a 37-year-old woman, severe precordial pressure and contusion of the thorax occurred after a collision. Cardiac symptoms developed two months later and two weeks after this acute myocardial infarction occurred. Coronary angiography demonstrated isolated sub-total occlusion of the anterior interventricular branch in the upper third of the septum without other abnormalities. Because of the two month symptom-free interval, trauma and subsequent myocardial infarction are thought not to be causally related, especially as the patient was a heavy smoker and taking oral contraceptives.", "contents": "[Myocardial infarction after accidents (author's transl)]. In a 27-year-old man blunt chest-wall trauma after a car accident gave rise to several retrosternal pain. Coronary angiography demonstrated severe generalised coronary arteriosclerosis. The history revealed heavy smoking (60 cigarettes daily for ten years). Although it must be assumed that there was severe generalised coronary arteriosclerosis without angina pectoris before the accident, the infarction was considered to be a direct consequence of it: it prematurely precipitated the infarction. In a second case, of a 37-year-old woman, severe precordial pressure and contusion of the thorax occurred after a collision. Cardiac symptoms developed two months later and two weeks after this acute myocardial infarction occurred. Coronary angiography demonstrated isolated sub-total occlusion of the anterior interventricular branch in the upper third of the septum without other abnormalities. Because of the two month symptom-free interval, trauma and subsequent myocardial infarction are thought not to be causally related, especially as the patient was a heavy smoker and taking oral contraceptives.", "PMID": 976112}
{"id": "pubmed23n0032_10558", "title": "[Yersinia arthritis (author's transl)].", "content": "Yersinia arthritis is an acute oligo- or, more rarely, polyarthritis. It occurs after pyrexic diarrhoea of several days, often associated with right-sided lower abdominal pain. The disease is caused by Yersinia enterocolitica and four such cases, confirmed serologically in three, are described. In one case fluid from the knee-joint was antibody-positive. It was not possible to culture the causative organism, probably because all patients had previously been treated with an antibiotic. Rheumatic fever and Reiter's disease must be considered in the differential diagnosis. Because pharyngitis, transitory ECG changes and conjunctivitis may occur in the early stages of Yersinia arthritis, the diagnosis may be missed. Prognosis of Yersinia arthritis is good, with complete cure within weeks or months. Demonstration of HLA-B 27 in all four cases points to a genetic disposition.", "contents": "[Yersinia arthritis (author's transl)]. Yersinia arthritis is an acute oligo- or, more rarely, polyarthritis. It occurs after pyrexic diarrhoea of several days, often associated with right-sided lower abdominal pain. The disease is caused by Yersinia enterocolitica and four such cases, confirmed serologically in three, are described. In one case fluid from the knee-joint was antibody-positive. It was not possible to culture the causative organism, probably because all patients had previously been treated with an antibiotic. Rheumatic fever and Reiter's disease must be considered in the differential diagnosis. Because pharyngitis, transitory ECG changes and conjunctivitis may occur in the early stages of Yersinia arthritis, the diagnosis may be missed. Prognosis of Yersinia arthritis is good, with complete cure within weeks or months. Demonstration of HLA-B 27 in all four cases points to a genetic disposition.", "PMID": 976116}
{"id": "pubmed23n0032_10559", "title": "[Traumatic subluxation of the head of the radius (author's transl)].", "content": "Radial-head subluxations were noted in 194 patients in the course of two years. It was more frequent in girls than boys (57.7% and 42.3%). The highest incidence was between the first and fourth year of life, with a peak in the first and second years. It no longer occurs after the seventh year. In 82.5% of cases the history was typical: a sudden longitudinal pull of the affected arm. In the remaining 17.5% no exact event could be ascertained. A questionnaire sent to the attending doctors provided replies in 82 cases (42.3%). Reposition by pull and pressure on the head of the radius, supination and acute flexing achieved repositioning in 92.7%, while in 7.3% repeat repositioning was necessary on the same or the next day. After successful repositioning complete absence of symptoms occurred in 98.8%. Incidence of recurrence was 30.5% during a follow-up period of up to a year and a half. The diagnosis had at first been had at first been missed 9.8% of cases.", "contents": "[Traumatic subluxation of the head of the radius (author's transl)]. Radial-head subluxations were noted in 194 patients in the course of two years. It was more frequent in girls than boys (57.7% and 42.3%). The highest incidence was between the first and fourth year of life, with a peak in the first and second years. It no longer occurs after the seventh year. In 82.5% of cases the history was typical: a sudden longitudinal pull of the affected arm. In the remaining 17.5% no exact event could be ascertained. A questionnaire sent to the attending doctors provided replies in 82 cases (42.3%). Reposition by pull and pressure on the head of the radius, supination and acute flexing achieved repositioning in 92.7%, while in 7.3% repeat repositioning was necessary on the same or the next day. After successful repositioning complete absence of symptoms occurred in 98.8%. Incidence of recurrence was 30.5% during a follow-up period of up to a year and a half. The diagnosis had at first been had at first been missed 9.8% of cases.", "PMID": 976117}
{"id": "pubmed23n0032_10560", "title": "[Kearns' syndrome (author's transl)].", "content": "Kearns' syndrome, a rare cause of chronic progressive ophthalmoplegia was observed in three patients aged 15 to 54 years. Apart from the chronic progressive external ophthalmoplegia the syndrome consists of retinal changes and cardiac conduction defects in all cases, as well as other signs indicating damage to the nervous system. High tone deafness and vestibular damage as well as an increase in CSF protein are common. Endocrine disorders and skeletal anomalies may occur. The cause is unknown. The ophthalmoplegia may be of neurogenic origin. Adams-Stokes attacks following disturbances of cardiac rhythm may be prevented by timely implantation of a cardiac pacemaker.", "contents": "[Kearns' syndrome (author's transl)]. Kearns' syndrome, a rare cause of chronic progressive ophthalmoplegia was observed in three patients aged 15 to 54 years. Apart from the chronic progressive external ophthalmoplegia the syndrome consists of retinal changes and cardiac conduction defects in all cases, as well as other signs indicating damage to the nervous system. High tone deafness and vestibular damage as well as an increase in CSF protein are common. Endocrine disorders and skeletal anomalies may occur. The cause is unknown. The ophthalmoplegia may be of neurogenic origin. Adams-Stokes attacks following disturbances of cardiac rhythm may be prevented by timely implantation of a cardiac pacemaker.", "PMID": 976118}
{"id": "pubmed23n0032_10561", "title": "[Carcinoid tumours of the rectum (author's transl)].", "content": "In three patients aged 21 to 65 rectal polyps diagnosed at rectoscopy were removed by diathermy loop. Histology showed them to be carcinoid tumours, one of tubular type, two of mixed type. Rectal carcinoid rarely presents with typical clinical symptoms. Polyps discovered by digital examination or rectoscopy should be considered to be potentially malignant and removed in toto. The size of the tumour is of decisive prognostic importance. Tumours with a cross section over 2 cm should be treated as malignant. The histological investigation generally gives no indication of clinical prognosis or of the possibility of metastases. Despite invasive growth and despite local or distant metastases carcinoid tumours can be judged more favourably than carcinoma. The plan of treatment should be according to the clinical findings as well, and not only to histological results. Postoperative follow-up is considered imperative.", "contents": "[Carcinoid tumours of the rectum (author's transl)]. In three patients aged 21 to 65 rectal polyps diagnosed at rectoscopy were removed by diathermy loop. Histology showed them to be carcinoid tumours, one of tubular type, two of mixed type. Rectal carcinoid rarely presents with typical clinical symptoms. Polyps discovered by digital examination or rectoscopy should be considered to be potentially malignant and removed in toto. The size of the tumour is of decisive prognostic importance. Tumours with a cross section over 2 cm should be treated as malignant. The histological investigation generally gives no indication of clinical prognosis or of the possibility of metastases. Despite invasive growth and despite local or distant metastases carcinoid tumours can be judged more favourably than carcinoma. The plan of treatment should be according to the clinical findings as well, and not only to histological results. Postoperative follow-up is considered imperative.", "PMID": 976119}
{"id": "pubmed23n0032_10562", "title": "[Continuous monitoring of pO2 by means of intravascular indwelling oxygen electrodes (author's transl)].", "content": "Two intravascular oxygen electrodes (of the International Biophysics Corporation and Roche) were tested in vitro and in long-term monitoring of arterial oxygen partial pressure in 19 patients. There were no complications. Both electrodes proved suitable for use for monitoring in an intensive-care situation. But both could still be improved by having greater stability and a shorter response time. Some of the indications for continuous monitoring of pO2 are mentioned: incipient respiratory failure, shock-lung, pulmonary oedema, and artificial ventilation. Haemorrhagic diatheses and arterial occlusive disease are contra-indications to its use.", "contents": "[Continuous monitoring of pO2 by means of intravascular indwelling oxygen electrodes (author's transl)]. Two intravascular oxygen electrodes (of the International Biophysics Corporation and Roche) were tested in vitro and in long-term monitoring of arterial oxygen partial pressure in 19 patients. There were no complications. Both electrodes proved suitable for use for monitoring in an intensive-care situation. But both could still be improved by having greater stability and a shorter response time. Some of the indications for continuous monitoring of pO2 are mentioned: incipient respiratory failure, shock-lung, pulmonary oedema, and artificial ventilation. Haemorrhagic diatheses and arterial occlusive disease are contra-indications to its use.", "PMID": 976126}
{"id": "pubmed23n0032_10563", "title": "[Anomalous aortic origin of a coronary artery: cause of sudden death in a 10-year-old girl (author's transl)].", "content": "A ten-year-old girl suddenly died on a playground, after repeated syncopal attacks during the preceding two years. Post-mortem examination revealed the left coronary artery to have arisen by a separate ostium from the right sinus of Valsalva, then coursing to the left between the route of the aorta and the pulmonary trunk where it divided as usual. There was no narrowing of the coronary-artery lumen and the wall was normal. The sudden death is explained by a rise in pressure in the root of the aorta with valve-like closure of the coronary artery. This anomaly should be thought of in other wise unexplained syncopal attacks in children or juveniles during physical exertion. The exercise ECG is apparently not helpful diagnostically. Operative treatment is possible.", "contents": "[Anomalous aortic origin of a coronary artery: cause of sudden death in a 10-year-old girl (author's transl)]. A ten-year-old girl suddenly died on a playground, after repeated syncopal attacks during the preceding two years. Post-mortem examination revealed the left coronary artery to have arisen by a separate ostium from the right sinus of Valsalva, then coursing to the left between the route of the aorta and the pulmonary trunk where it divided as usual. There was no narrowing of the coronary-artery lumen and the wall was normal. The sudden death is explained by a rise in pressure in the root of the aorta with valve-like closure of the coronary artery. This anomaly should be thought of in other wise unexplained syncopal attacks in children or juveniles during physical exertion. The exercise ECG is apparently not helpful diagnostically. Operative treatment is possible.", "PMID": 976127}
{"id": "pubmed23n0032_10564", "title": "Management of the acute attack of asthma in general practice.", "content": "Many acute attacks of asthma respond readily and completely to aerosol and/or parenteral bronchodilators, and subsequent management of asthma should try to prevent a further acute recurrence. Explanation of drug administration techniques and of the general nature of asthma are of great value in achieving good control. Severe, prolonged attacks require energetic measures, with transfer to units with intensive care facilities. These life-threatening situations are generally not hard to recognise.", "contents": "Management of the acute attack of asthma in general practice. Many acute attacks of asthma respond readily and completely to aerosol and/or parenteral bronchodilators, and subsequent management of asthma should try to prevent a further acute recurrence. Explanation of drug administration techniques and of the general nature of asthma are of great value in achieving good control. Severe, prolonged attacks require energetic measures, with transfer to units with intensive care facilities. These life-threatening situations are generally not hard to recognise.", "PMID": 976133}
{"id": "pubmed23n0032_10565", "title": "Clonazepam: a review of its pharmacological properties and therapeutic efficacy in epilepsy.", "content": "Clonazepam or 5-(2-chlorphenyl)-1, 3-dihydro-7-nitro-2H-1,4benzodiazepin-2-one, is a close structural and pharmacological relative of nitrazepam. It has a broad spectrum of activity against the various types of epilepsy, and is effective in many patients whose condition has proved resistant to other antiepileptic drugs. Its chief uses are in status epilepticus, in which intravenous clonazepam may replace diazepam as the drug of first choice, and in the minor motor seizures of childhood, particularly petit mal absences, the Lennox-Gastaut syndrome and infantile spasms. Clonazepam is also at least as effective as current treatment in psychomotor and myoclonic epilepsies, but seems unlikely to replace phenytoin and the barbiturates in the treatment of grand mal or focal motor seizures except in patients resistant to standard therapy. Initial success with clonazepam can be followed by loss of effect, but benefit can often be restored, at least initially, by temporary interruption and re-institution of treatment. Side-effects are common with clonazepam. Most patients experience drowsiness and fatigue, which are frequent causes of withdrawal, together with lesser incidences of ataxia, dystonia, hypotonia, and hyperactivity. These effects usually disappear with continued therapy, and are minimised by gradual introduction of the drug over 2-4 weeks. Hypersalivation and excessive bronchial secretion may be a problem in children and infants.", "contents": "Clonazepam: a review of its pharmacological properties and therapeutic efficacy in epilepsy. Clonazepam or 5-(2-chlorphenyl)-1, 3-dihydro-7-nitro-2H-1,4benzodiazepin-2-one, is a close structural and pharmacological relative of nitrazepam. It has a broad spectrum of activity against the various types of epilepsy, and is effective in many patients whose condition has proved resistant to other antiepileptic drugs. Its chief uses are in status epilepticus, in which intravenous clonazepam may replace diazepam as the drug of first choice, and in the minor motor seizures of childhood, particularly petit mal absences, the Lennox-Gastaut syndrome and infantile spasms. Clonazepam is also at least as effective as current treatment in psychomotor and myoclonic epilepsies, but seems unlikely to replace phenytoin and the barbiturates in the treatment of grand mal or focal motor seizures except in patients resistant to standard therapy. Initial success with clonazepam can be followed by loss of effect, but benefit can often be restored, at least initially, by temporary interruption and re-institution of treatment. Side-effects are common with clonazepam. Most patients experience drowsiness and fatigue, which are frequent causes of withdrawal, together with lesser incidences of ataxia, dystonia, hypotonia, and hyperactivity. These effects usually disappear with continued therapy, and are minimised by gradual introduction of the drug over 2-4 weeks. Hypersalivation and excessive bronchial secretion may be a problem in children and infants.", "PMID": 976134}
{"id": "pubmed23n0032_10566", "title": "Suppression of the estradiol receptor system by progesterone in the oviduct and uterus of the cat.", "content": "Four weeks or more after being spayed, cats were either left untreated or treated with various regimens of estradiol (E2) and progesterone (P) in silastic implants. Oviducts and uteri were then removed and examined for morphological changes as well as for the amount and compartmentalization of the estrogen receptor. E2 treatment restored a full complement of ciliated and secretory cells to the oviduct, and subsequent P treatment (with or without continuous E2) of an E2-restored oviduct produced an atrophied epithelium equivalent to that resulting from E2 withdrawal. In the uterus, P treatment after E2 priming did not lead to E2-withdrawal symptoms but rather to a new stage of differentiation characterized by altered and increased secretory activity. In both organs E2 caused dramatic elevations in the E2-receptor content in the nuclear as well as in the cytoplasmic compartments. Also (in both organs) P treatment after E2 priming reduced the E2-receptor content in the nuclear and cytoplasmic compartments to the levels present in spayed cats. The degree of suppression caused by P in both organs was similar to that induced by E2 withdrawal. Suppression of the E2-receptor system by P correlated with suppressed function inthe oviduct but with increased growth and secretory activity in the uterus.", "contents": "Suppression of the estradiol receptor system by progesterone in the oviduct and uterus of the cat. Four weeks or more after being spayed, cats were either left untreated or treated with various regimens of estradiol (E2) and progesterone (P) in silastic implants. Oviducts and uteri were then removed and examined for morphological changes as well as for the amount and compartmentalization of the estrogen receptor. E2 treatment restored a full complement of ciliated and secretory cells to the oviduct, and subsequent P treatment (with or without continuous E2) of an E2-restored oviduct produced an atrophied epithelium equivalent to that resulting from E2 withdrawal. In the uterus, P treatment after E2 priming did not lead to E2-withdrawal symptoms but rather to a new stage of differentiation characterized by altered and increased secretory activity. In both organs E2 caused dramatic elevations in the E2-receptor content in the nuclear as well as in the cytoplasmic compartments. Also (in both organs) P treatment after E2 priming reduced the E2-receptor content in the nuclear and cytoplasmic compartments to the levels present in spayed cats. The degree of suppression caused by P in both organs was similar to that induced by E2 withdrawal. Suppression of the E2-receptor system by P correlated with suppressed function inthe oviduct but with increased growth and secretory activity in the uterus.", "PMID": 976186}
{"id": "pubmed23n0032_10567", "title": "Relationship between membrane potential and progesterone release in ovine corpora lutea.", "content": "When slices of ovine luteal tissue were perfused with medium containing luteinizing hormone (LH), the output of progesterone was increased significantly (P less than 0.01) in eleven of twelve experiments. However, addition of LH to the medium did not influence the luteal cell membrane potential. The addition of 47 mM potassium to the medium resulted in increased progesterone output (P less than 0.01) and depolarization of the luteal cell membrane within 2 min. Progesterone output decreased to approximate pretreatment levels within 2 min of the return to normal potassium levels in the perfusion medium. High levels of potassium further increased the output of progesterone from tissue stimulated with LH. Perfusion of the slices with sodium-free medium also resulted in increased (P less than 0.01) progesterone output within 2 min, which returned to pretreatment levels within 2 min after normal sodium levels were restored to the medium. Perfusion of the slices with sodium-free medium did not influence the membrane potential. Perfusion of the tissue with LH, 47 mM potassium, or sodium-free medium had no effect on progesterone output if the medium was calcium-free and/or contained 2 mM EGTA. These data suggested that the calcium ion plays an important role in mediating the steroidogenic response of ovine luteal tissue to LH. A second series of experiments was designed to ascertain if luteal cells were coupled electrically. Sixty-six pairs of luteal cells separated by 150-300 mum were penetrated with electrodes and the membrane potential of both cells was studied. One cell of each pair was hyperpolarized by passage of 0.4 nA current into the cell, but in no case was there an effect on the membrane potential of the other penetrated cell. Likewise, when five cells were injected iontophoretically with Procion Yellow there was no evidence of diffusion of the dye to adjacent cells. There was no evidence obtained in this study which suggested that ovine luteal cells were coupled electrically.", "contents": "Relationship between membrane potential and progesterone release in ovine corpora lutea. When slices of ovine luteal tissue were perfused with medium containing luteinizing hormone (LH), the output of progesterone was increased significantly (P less than 0.01) in eleven of twelve experiments. However, addition of LH to the medium did not influence the luteal cell membrane potential. The addition of 47 mM potassium to the medium resulted in increased progesterone output (P less than 0.01) and depolarization of the luteal cell membrane within 2 min. Progesterone output decreased to approximate pretreatment levels within 2 min of the return to normal potassium levels in the perfusion medium. High levels of potassium further increased the output of progesterone from tissue stimulated with LH. Perfusion of the slices with sodium-free medium also resulted in increased (P less than 0.01) progesterone output within 2 min, which returned to pretreatment levels within 2 min after normal sodium levels were restored to the medium. Perfusion of the slices with sodium-free medium did not influence the membrane potential. Perfusion of the tissue with LH, 47 mM potassium, or sodium-free medium had no effect on progesterone output if the medium was calcium-free and/or contained 2 mM EGTA. These data suggested that the calcium ion plays an important role in mediating the steroidogenic response of ovine luteal tissue to LH. A second series of experiments was designed to ascertain if luteal cells were coupled electrically. Sixty-six pairs of luteal cells separated by 150-300 mum were penetrated with electrodes and the membrane potential of both cells was studied. One cell of each pair was hyperpolarized by passage of 0.4 nA current into the cell, but in no case was there an effect on the membrane potential of the other penetrated cell. Likewise, when five cells were injected iontophoretically with Procion Yellow there was no evidence of diffusion of the dye to adjacent cells. There was no evidence obtained in this study which suggested that ovine luteal cells were coupled electrically.", "PMID": 976187}
{"id": "pubmed23n0032_10568", "title": "Adrenal mitochondrial and serum corticosteroid studies in rats resistant to adrenal-regeneration hypertension (ARH).", "content": "Female rats of the Wistar-Furth (W/Fu) strain appear to be resistant to the development of adrenal regeneration hypertension. At a time period, after adrenal enucleation, when Holtzman female rats had elevated serum 11-deoxycorticosterone levels and were hypertensive, none of the W/Fu rats became hypertensive. In vitro adrenal studies after quiescent kills of W/Fu rats indicated that cholesterol side chain cleavage activity was greater in mitochondria from regenerating adrenals than from controls. Both serum deoxycorticosterone and corticosterone levels were significantly greater in the adrenal-enucleated group. These studies were repeated in animals which were given a standard ether anesthetic stress. Ether stress increased cholesterol side chain cleavage activity comparably in control and adrenal-enucleated rats and also increased their serum deoxycorticosterone and corticosterone levels. Adrenal-enucleated Wistar-Furth rats had higher serum deoxycorticosterone levels than controls, whereas controls had higher serum corticosterone levels than the adrenal-enucleated group after the ether stress. These results indicate that although the adrenal-enucleated W/Fu rats have increased serum deoxycorticosterone levels, none of these rats develop frank hypertension. This suggests a resistance to deoxycorticosterone-induced hypertension in this strain of rat.", "contents": "Adrenal mitochondrial and serum corticosteroid studies in rats resistant to adrenal-regeneration hypertension (ARH). Female rats of the Wistar-Furth (W/Fu) strain appear to be resistant to the development of adrenal regeneration hypertension. At a time period, after adrenal enucleation, when Holtzman female rats had elevated serum 11-deoxycorticosterone levels and were hypertensive, none of the W/Fu rats became hypertensive. In vitro adrenal studies after quiescent kills of W/Fu rats indicated that cholesterol side chain cleavage activity was greater in mitochondria from regenerating adrenals than from controls. Both serum deoxycorticosterone and corticosterone levels were significantly greater in the adrenal-enucleated group. These studies were repeated in animals which were given a standard ether anesthetic stress. Ether stress increased cholesterol side chain cleavage activity comparably in control and adrenal-enucleated rats and also increased their serum deoxycorticosterone and corticosterone levels. Adrenal-enucleated Wistar-Furth rats had higher serum deoxycorticosterone levels than controls, whereas controls had higher serum corticosterone levels than the adrenal-enucleated group after the ether stress. These results indicate that although the adrenal-enucleated W/Fu rats have increased serum deoxycorticosterone levels, none of these rats develop frank hypertension. This suggests a resistance to deoxycorticosterone-induced hypertension in this strain of rat.", "PMID": 976188}
{"id": "pubmed23n0032_10569", "title": "Mechanism of interaction of digitalis with estradiol binding sites in rat uteri.", "content": "Digitalis preparations have a weak estrogenic effect in man. The data in animals are equivocal. We have studied.the biologic effect of both digitoxin and digoxin on the rat uterus in vivo and the interaction of these drugs with the rat uterus estrogen receptor in vitro. Digitoxin and estradiol significantly increased the uterine weight of immature rats, while digoxin did not. The interaction of digitoxin and digoxin with the rat uterus estrogen cytosol receptor was studied using protamine sulfate precipitation and dextran-coated charcoal (DCC) assays. Both methods gave a Kd for the estradiol-receptor interaction between 0.8-3.1 X 10(-9) M (n = 20). Digitoxin at concentrations of 0.5-2.0 X 10(-6) M significantly inhibited the binding of estradiol to the specific or saturable binding sites with minimal inhibition of hormonal binding to nonspecific sites. The binding was competitive with a calculated Ki for digitoxin of 5.2-7.8 X 10(-7) M (n = 18). Digoxin failed to inhibit estradiol binding to the receptor protein in vitro. We conclude that digitoxin probably acts directly as a weak estrogen and that this effect probably explains the estrogen-like side effects seen with digitoxin therapy in man.", "contents": "Mechanism of interaction of digitalis with estradiol binding sites in rat uteri. Digitalis preparations have a weak estrogenic effect in man. The data in animals are equivocal. We have studied.the biologic effect of both digitoxin and digoxin on the rat uterus in vivo and the interaction of these drugs with the rat uterus estrogen receptor in vitro. Digitoxin and estradiol significantly increased the uterine weight of immature rats, while digoxin did not. The interaction of digitoxin and digoxin with the rat uterus estrogen cytosol receptor was studied using protamine sulfate precipitation and dextran-coated charcoal (DCC) assays. Both methods gave a Kd for the estradiol-receptor interaction between 0.8-3.1 X 10(-9) M (n = 20). Digitoxin at concentrations of 0.5-2.0 X 10(-6) M significantly inhibited the binding of estradiol to the specific or saturable binding sites with minimal inhibition of hormonal binding to nonspecific sites. The binding was competitive with a calculated Ki for digitoxin of 5.2-7.8 X 10(-7) M (n = 18). Digoxin failed to inhibit estradiol binding to the receptor protein in vitro. We conclude that digitoxin probably acts directly as a weak estrogen and that this effect probably explains the estrogen-like side effects seen with digitoxin therapy in man.", "PMID": 976189}
{"id": "pubmed23n0032_10570", "title": "Effects of spironolactone, canrenone and canrenoate-K on cytochrome P450, and 11beta- and 18-hydroxylation in bovine and human adrenal cortical mitochondria.", "content": "Effects of spironolactone, canrenone and canrenoate-K on adrenal cytochrome P450 (P450) and corticosteroid biosynthesis were examined by studying difference spectra, P450 reduction and corticoid hydroxylation in mitochondrial preparations isolated from zona fasciculata and zona glomerulosa of bovine adrenals and from adrenal adenoma and hyperplastic adrenal cortex removed from patients with hyperaldosteronism. All three agents bound to P450 producing type I difference spectra and underwent hydroxylation. They all inhibited 11beta-hydroxylation in bovine adrenal at 30 muM and higher concentrations. Canrenone, the most potent inhibitor, blocked enzyme activity by 60% at a concentration of 60 muM. Spironolactone stimulated P450 reduction. The order of potency of inhibition was found to correlate with the order of affinity of these agents for P450. 11beta-Hydroxylase in human adrenal appeared to be less sensitive to canrenone. All three agents or their hydroxylated metabolites blocked 18-hydroxylation in bovine adrenal at lower concentrations. Canrenoate-K, being the most effective, inhibited 52% at 20 muM. Low concentrations of canrenone (2.5-5.0 muM) were without effect on 11beta-hydroxylase but markedly inhibited 18-hydroxylation (62-76%) in hyperplastic human adrenals. The inhibitors produced mixed type inhibition of 11beta-hydroxylation and competitive type inhibition of 18-hydroxylation. These findings indicate that at low concentrations spironolactone and its major metabolites, canrenone and canrenoate-K, or their hydroxylated metabolites, can directly interfere with the biosynthesis of aldosterone in bovine and certain human adrenal cortical tissue.", "contents": "Effects of spironolactone, canrenone and canrenoate-K on cytochrome P450, and 11beta- and 18-hydroxylation in bovine and human adrenal cortical mitochondria. Effects of spironolactone, canrenone and canrenoate-K on adrenal cytochrome P450 (P450) and corticosteroid biosynthesis were examined by studying difference spectra, P450 reduction and corticoid hydroxylation in mitochondrial preparations isolated from zona fasciculata and zona glomerulosa of bovine adrenals and from adrenal adenoma and hyperplastic adrenal cortex removed from patients with hyperaldosteronism. All three agents bound to P450 producing type I difference spectra and underwent hydroxylation. They all inhibited 11beta-hydroxylation in bovine adrenal at 30 muM and higher concentrations. Canrenone, the most potent inhibitor, blocked enzyme activity by 60% at a concentration of 60 muM. Spironolactone stimulated P450 reduction. The order of potency of inhibition was found to correlate with the order of affinity of these agents for P450. 11beta-Hydroxylase in human adrenal appeared to be less sensitive to canrenone. All three agents or their hydroxylated metabolites blocked 18-hydroxylation in bovine adrenal at lower concentrations. Canrenoate-K, being the most effective, inhibited 52% at 20 muM. Low concentrations of canrenone (2.5-5.0 muM) were without effect on 11beta-hydroxylase but markedly inhibited 18-hydroxylation (62-76%) in hyperplastic human adrenals. The inhibitors produced mixed type inhibition of 11beta-hydroxylation and competitive type inhibition of 18-hydroxylation. These findings indicate that at low concentrations spironolactone and its major metabolites, canrenone and canrenoate-K, or their hydroxylated metabolites, can directly interfere with the biosynthesis of aldosterone in bovine and certain human adrenal cortical tissue.", "PMID": 976190}
{"id": "pubmed23n0032_10571", "title": "The interrelation of renin and iron binding capacity.", "content": "When rats are subjected to hypoxia, an increase in serum angiotensinogen concentration occurs which is accompanied by the appearance of serum erythropoietin (EP) during the first 24 h. Subsequent increases in EP reach maximum values 24 to 48 h after increases in packed cell volume (PCV) and serum renin levels. The current experiments were designed to determine if a decrease in iron stores is the stimulus for renin production when rats are rapidly expanding their red cell volume in a hypoxic environment. Young rats fed McCall's low iron diet were paired with rats fed the same diet supplemented with ferric citrate (6 g/kg diet). After two weeks at ambient pressure, they were subjected to hypoxia (0.48 atm) for 1 to 10 days. After 5 days at the reduced pressure, a fraction of the rats on the low iron diet were fed the iron-supplemented diet. At the time of sacrifice, serum was assayed for total iron binding capacity (TIBC) and renin. Rats that were fed the low iron diet showed an increase in TIBC, an increase in serum renin and a positive correlation between serum renin and TIBC. Rats that were fed a normal iron diet under the same conditions had lower TIBC, lower serum renin and no correlation between serum renin and TIBC. When low iron diet rats were supplemented with iron, TIBC and serum renin decreased. These experiments may have a clinical counterpart. The total iron binding capacity, renin and angiotensinogen were measured in the serum of women during the first 19 weeks of pregnancy. Women during early pregnancy showed an increase in TIBC, an increase of renin and angiotensinogen in the serum and a positive correlation between TIBC and renin concentration. It is suggested that the increased concentration of renin in the serum of women during the first 19 weeks of pregnancy and in the serum of rats that are rapidly expanding their red cell volume are related to a decrease in iron stores.", "contents": "The interrelation of renin and iron binding capacity. When rats are subjected to hypoxia, an increase in serum angiotensinogen concentration occurs which is accompanied by the appearance of serum erythropoietin (EP) during the first 24 h. Subsequent increases in EP reach maximum values 24 to 48 h after increases in packed cell volume (PCV) and serum renin levels. The current experiments were designed to determine if a decrease in iron stores is the stimulus for renin production when rats are rapidly expanding their red cell volume in a hypoxic environment. Young rats fed McCall's low iron diet were paired with rats fed the same diet supplemented with ferric citrate (6 g/kg diet). After two weeks at ambient pressure, they were subjected to hypoxia (0.48 atm) for 1 to 10 days. After 5 days at the reduced pressure, a fraction of the rats on the low iron diet were fed the iron-supplemented diet. At the time of sacrifice, serum was assayed for total iron binding capacity (TIBC) and renin. Rats that were fed the low iron diet showed an increase in TIBC, an increase in serum renin and a positive correlation between serum renin and TIBC. Rats that were fed a normal iron diet under the same conditions had lower TIBC, lower serum renin and no correlation between serum renin and TIBC. When low iron diet rats were supplemented with iron, TIBC and serum renin decreased. These experiments may have a clinical counterpart. The total iron binding capacity, renin and angiotensinogen were measured in the serum of women during the first 19 weeks of pregnancy. Women during early pregnancy showed an increase in TIBC, an increase of renin and angiotensinogen in the serum and a positive correlation between TIBC and renin concentration. It is suggested that the increased concentration of renin in the serum of women during the first 19 weeks of pregnancy and in the serum of rats that are rapidly expanding their red cell volume are related to a decrease in iron stores.", "PMID": 976191}
{"id": "pubmed23n0032_10572", "title": "Follicle stimulating activity of human chorionic gonadotropin: effect of dissociation and recombination of subunits.", "content": "The follicle stimulating activity (FSA) and interstitial cell stimulating activity (ICSA) of highly purified human chorionic gonadotropin (hCG), its alpha and beta subunits, and hCG generated by subunit recombination were determined by ovarian weight and ventral prostate weight bioassays. Whereas highly purified hCG exhibited both FA and ICSA, its separated subunits were essentially devoid of both activities. ICSA and FSA, indistinguishable from that of the highly purified hCG, were restored by recombination of the hCG subunits. These observations are consistent with the hypothesis that the FSA and ICSA found in highly purified hCG preparations are properties of the hCG molecule.", "contents": "Follicle stimulating activity of human chorionic gonadotropin: effect of dissociation and recombination of subunits. The follicle stimulating activity (FSA) and interstitial cell stimulating activity (ICSA) of highly purified human chorionic gonadotropin (hCG), its alpha and beta subunits, and hCG generated by subunit recombination were determined by ovarian weight and ventral prostate weight bioassays. Whereas highly purified hCG exhibited both FA and ICSA, its separated subunits were essentially devoid of both activities. ICSA and FSA, indistinguishable from that of the highly purified hCG, were restored by recombination of the hCG subunits. These observations are consistent with the hypothesis that the FSA and ICSA found in highly purified hCG preparations are properties of the hCG molecule.", "PMID": 976192}
{"id": "pubmed23n0032_10573", "title": "Stimulation of aromatization of exogenous and endogenous androgens in ovaries of hypophysectomized rats in vivo by follicle-stimulating hormone.", "content": "The role of follicle-stimulating hormone (FSH) in the regulation of estrogen biosynthesis in vivo has been investigated in immature hypophysectomized rats, utilizing uterine weight and histologic responses, and ovarian estradiol-17beta concentrations as indicators of estrogen secretion. A highly purified FSH preparation produced only borderline increases in uterine weights and in ovarian estradiol-17beta contents when administered alone at 2.5 mug/day for 3 days. Testosterone or androstenedione (5 mg/day), when administered in the absence of FSH, produced typically androgenic stimulation of uteri, and did not increase ovarian estradiol-17beta concentrations. When administered concomitantly with FSH, the uterine weight-stimulating activity of these aromatizable androgens was substantially increased, accompanied by marked hypertrophy of the endometrial mucosal cells, and ovarian estradiol-17beta concentrations were increased 20- to 200-fold. The administration of the non-aromatizable androgen, 17beta-OH-5alpha-androstan-3-one (DHT) (5 mg/day, by itself produced uterine weight increases similar to those caused by testosterone alone; however, no evidence of increased estrogen secretion resulted from the combined treatment of DHT wtih FSH. A highly purified luteinizing hormone (LH) preparation was equally as effective as exogenous androstenedione in increasing ovarian concentrations of immunoreactive androgen (testosterone + DHT) but evoked none of the above signs of estrogen secretion unless administered together with FSH. The weights of ovaries were not affected by the administration of LH or of any of the androgens by themselves, but were approximately doubled by FSH alone. Ovarian weights were increased still further when FSH was administered concomitantly with LH, testosterone , or androstenedione, but not with DHT. It is concluded that FSH and LH regulate ovarian estrogen secretion in vivo by acting at biochemically distinct sites--LH stimulating the synthesis of C19-steroids, which are then converted to estradiol-17beta under specific stimulation by FSH.", "contents": "Stimulation of aromatization of exogenous and endogenous androgens in ovaries of hypophysectomized rats in vivo by follicle-stimulating hormone. The role of follicle-stimulating hormone (FSH) in the regulation of estrogen biosynthesis in vivo has been investigated in immature hypophysectomized rats, utilizing uterine weight and histologic responses, and ovarian estradiol-17beta concentrations as indicators of estrogen secretion. A highly purified FSH preparation produced only borderline increases in uterine weights and in ovarian estradiol-17beta contents when administered alone at 2.5 mug/day for 3 days. Testosterone or androstenedione (5 mg/day), when administered in the absence of FSH, produced typically androgenic stimulation of uteri, and did not increase ovarian estradiol-17beta concentrations. When administered concomitantly with FSH, the uterine weight-stimulating activity of these aromatizable androgens was substantially increased, accompanied by marked hypertrophy of the endometrial mucosal cells, and ovarian estradiol-17beta concentrations were increased 20- to 200-fold. The administration of the non-aromatizable androgen, 17beta-OH-5alpha-androstan-3-one (DHT) (5 mg/day, by itself produced uterine weight increases similar to those caused by testosterone alone; however, no evidence of increased estrogen secretion resulted from the combined treatment of DHT wtih FSH. A highly purified luteinizing hormone (LH) preparation was equally as effective as exogenous androstenedione in increasing ovarian concentrations of immunoreactive androgen (testosterone + DHT) but evoked none of the above signs of estrogen secretion unless administered together with FSH. The weights of ovaries were not affected by the administration of LH or of any of the androgens by themselves, but were approximately doubled by FSH alone. Ovarian weights were increased still further when FSH was administered concomitantly with LH, testosterone , or androstenedione, but not with DHT. It is concluded that FSH and LH regulate ovarian estrogen secretion in vivo by acting at biochemically distinct sites--LH stimulating the synthesis of C19-steroids, which are then converted to estradiol-17beta under specific stimulation by FSH.", "PMID": 976193}
{"id": "pubmed23n0032_10574", "title": "Progesterone metabolism by the ovary of the pregnant rat: discrepancies in the catabolic regulation model.", "content": "The intraovarian site of 20 alpha-hydroxysteroid dehydrogenase activity (20 alpha-OH-SDH) was determined biochemically by measuring enzyme activity in homogenates of the whole ovary, or of isolated ovarian compartments, during the last third segment of pregnancy in the rat. In agreement, with previously reported histochemical evidence, an increase in 20 alpha-OH-SDH activity was observed in isolated corpora lutea, but not in the non-luteal compartment of the ovary. Enzyme activity in corpora lutea was low between days 16 and 22 of pregnancy, but increased markedly (4-6 fold) on day 23. Between days 17 and 20 of pregnancy, serum concentrations of progesterone declined from 130 +/- 3 to 80 +/- 3 ng/ml, while 20 alpha-hydroxypregn-4-en-3-one (20 alpha-OH-P) concentrations declined from 34 +/- 3 to 18.5 +/- 3 ng/ml. Only later, between days 20 and 22 of pregnancy, was a significant decline in serum progesterone concentrations associated with an increase in serum 20 alpha-OH-P concentrations (50 +/- 15 ng/ml at 0800 h and 128.5 +/- 15 ng/ml at 1400 h on day 22). Thus the decline in progesterone concentration late in pregnancy can be explained only partially by conversion of progesterone to 20 alpha-OH-P. Further, a dissociation between changes in enzyme activity and in serum concentrations of 20 alpha-OH-P was also observed. A marked increase in serum levels of 20 alpha-OH-P on day 22 preceded any increase in enzyme activity by at least 10 h, and continued increases in enzyme activity on day 23 were not associated with any steady increase in peripheral 20 alpha-OH-P levels. We conclude from these observations that luteal regression is a more complex phenomenon than the regulation of a single enzyme, 20 alpha-hydroxysteroid dehydrogenase, and may involve regulation of both the synthesis and degradation of progesterone.", "contents": "Progesterone metabolism by the ovary of the pregnant rat: discrepancies in the catabolic regulation model. The intraovarian site of 20 alpha-hydroxysteroid dehydrogenase activity (20 alpha-OH-SDH) was determined biochemically by measuring enzyme activity in homogenates of the whole ovary, or of isolated ovarian compartments, during the last third segment of pregnancy in the rat. In agreement, with previously reported histochemical evidence, an increase in 20 alpha-OH-SDH activity was observed in isolated corpora lutea, but not in the non-luteal compartment of the ovary. Enzyme activity in corpora lutea was low between days 16 and 22 of pregnancy, but increased markedly (4-6 fold) on day 23. Between days 17 and 20 of pregnancy, serum concentrations of progesterone declined from 130 +/- 3 to 80 +/- 3 ng/ml, while 20 alpha-hydroxypregn-4-en-3-one (20 alpha-OH-P) concentrations declined from 34 +/- 3 to 18.5 +/- 3 ng/ml. Only later, between days 20 and 22 of pregnancy, was a significant decline in serum progesterone concentrations associated with an increase in serum 20 alpha-OH-P concentrations (50 +/- 15 ng/ml at 0800 h and 128.5 +/- 15 ng/ml at 1400 h on day 22). Thus the decline in progesterone concentration late in pregnancy can be explained only partially by conversion of progesterone to 20 alpha-OH-P. Further, a dissociation between changes in enzyme activity and in serum concentrations of 20 alpha-OH-P was also observed. A marked increase in serum levels of 20 alpha-OH-P on day 22 preceded any increase in enzyme activity by at least 10 h, and continued increases in enzyme activity on day 23 were not associated with any steady increase in peripheral 20 alpha-OH-P levels. We conclude from these observations that luteal regression is a more complex phenomenon than the regulation of a single enzyme, 20 alpha-hydroxysteroid dehydrogenase, and may involve regulation of both the synthesis and degradation of progesterone.", "PMID": 976194}
{"id": "pubmed23n0032_10575", "title": "Pituitary thyrotropin (TSH) rebound phenomenon and kinetics of secretion in the goitrous rat: differential effects of thyroxine on synthesis and release of TSH.", "content": "The protein metabolism and [3H]-uridine uptake of thyroid and adenohypophysis and the kinetics of pituitary TSH rebound (PTR) were studied in goitrous female rats (fed propylthiouracil, PTU: for 7-12 weeks) following single, iv injections of L-thyroxine (T4: 0.8 to 200 mug). Goitrogenesis was associated with reduced protein concentration and enhanced uptake of [3H] uridine in both glands. Plasma levels of TSH were invariably elevated but stores in the adenohypophysis were consistently reduced. Small doses of T4 (4 mug) induced significant TSH repletion in the pituitary within 2-6 h following injection. Accumulations of pituitary TSH to supranormal levels (15-fold increases) were achieved with 20 mug T4 at 6 and 24 h; higher doses (100-200 mug) inhibited the PTR at all time intervals tested (0.5-24 h). Administration of puromycin or actinomycin D did not influence the PTR. Protein content and labeled uridine uptake of the pituitary bore no apparent relationship to T4-induced TSH repletion in the gland. Blood clearance rate of exogenous rat TSH was measured prior to and during PTR. Plasma half-life was determined to be 13.6 and 19.9 min in euthyroid and chronically hypothyroid rats, respectively; it was not significantly altered from the latter during rebound (18.7 min). Calculations of theoretical TSH secretory rates prior to (50.5 +/- 4.4 mU/h) and after rebound with 20 mug T4 (25.4 +/- 4.2 mU/H) revealed that the reaccumulation of TSH in the pituitary induced with T4 cannot be attributed solely to inhibition of release, but may also involve enhancement of synthesis. It is concluded that T4 administration at high dose levels inhibits both synthesis and release of TSH from pituitary thyrotrophs, whereas low critical doses of T4 suppress release, but augment synthesis and/or facilitate conformational change in a pituitary precursor(s) molecule which renders it detectable by bioassay.", "contents": "Pituitary thyrotropin (TSH) rebound phenomenon and kinetics of secretion in the goitrous rat: differential effects of thyroxine on synthesis and release of TSH. The protein metabolism and [3H]-uridine uptake of thyroid and adenohypophysis and the kinetics of pituitary TSH rebound (PTR) were studied in goitrous female rats (fed propylthiouracil, PTU: for 7-12 weeks) following single, iv injections of L-thyroxine (T4: 0.8 to 200 mug). Goitrogenesis was associated with reduced protein concentration and enhanced uptake of [3H] uridine in both glands. Plasma levels of TSH were invariably elevated but stores in the adenohypophysis were consistently reduced. Small doses of T4 (4 mug) induced significant TSH repletion in the pituitary within 2-6 h following injection. Accumulations of pituitary TSH to supranormal levels (15-fold increases) were achieved with 20 mug T4 at 6 and 24 h; higher doses (100-200 mug) inhibited the PTR at all time intervals tested (0.5-24 h). Administration of puromycin or actinomycin D did not influence the PTR. Protein content and labeled uridine uptake of the pituitary bore no apparent relationship to T4-induced TSH repletion in the gland. Blood clearance rate of exogenous rat TSH was measured prior to and during PTR. Plasma half-life was determined to be 13.6 and 19.9 min in euthyroid and chronically hypothyroid rats, respectively; it was not significantly altered from the latter during rebound (18.7 min). Calculations of theoretical TSH secretory rates prior to (50.5 +/- 4.4 mU/h) and after rebound with 20 mug T4 (25.4 +/- 4.2 mU/H) revealed that the reaccumulation of TSH in the pituitary induced with T4 cannot be attributed solely to inhibition of release, but may also involve enhancement of synthesis. It is concluded that T4 administration at high dose levels inhibits both synthesis and release of TSH from pituitary thyrotrophs, whereas low critical doses of T4 suppress release, but augment synthesis and/or facilitate conformational change in a pituitary precursor(s) molecule which renders it detectable by bioassay.", "PMID": 976195}
{"id": "pubmed23n0032_10576", "title": "Effect of surgical disconnection of the medial basal hypothalamus on postcoital reflex ovulation in the rabbit.", "content": "The effects of hypothalamic transections of varying lengths on reflex ovulation in the rabbit are reported. Circular cuts which surgically isolated the medial basal hypothalamus (MBH) from the remainder of the brain did not prevent copulation 4 to 24 h later, but did block reflex ovulation. On the contrary, hemicircular cuts of either the rostral, caudal or one lateral haf of the circular cut did not prevent reflex ovulation. These results suggest that coital stimuli reach the MBH through a diffuse network of fibers and that only very extensive deaferrentation of the MBH can cause blockade of ovulation in rabbits. These data are compared with results of other investigators in which anterior deafferentation of the MBH blocked reflex and spontaneous ovulation in rats.", "contents": "Effect of surgical disconnection of the medial basal hypothalamus on postcoital reflex ovulation in the rabbit. The effects of hypothalamic transections of varying lengths on reflex ovulation in the rabbit are reported. Circular cuts which surgically isolated the medial basal hypothalamus (MBH) from the remainder of the brain did not prevent copulation 4 to 24 h later, but did block reflex ovulation. On the contrary, hemicircular cuts of either the rostral, caudal or one lateral haf of the circular cut did not prevent reflex ovulation. These results suggest that coital stimuli reach the MBH through a diffuse network of fibers and that only very extensive deaferrentation of the MBH can cause blockade of ovulation in rabbits. These data are compared with results of other investigators in which anterior deafferentation of the MBH blocked reflex and spontaneous ovulation in rats.", "PMID": 976196}
{"id": "pubmed23n0032_10577", "title": "Extrahypothalamic control of stress-induced inhibition of growth hormone secretion in the rat.", "content": "The aim of these experiments was to determine whether the telencephalon is essential for stress-induced suppression of plasma GH levels and, if so, which telencephalic structures are involved. In the first study, the telencephalon was completely removed from adult female rats by suction; intact and sham-operated rats served as controls. At 24 h after surgery, blood samples were collected at the initiation of and 15 min after a 3-min immobilization-blood withdrawal stress procedure and used for determining non-stress and stress plasma levels of GH, respectively. GH was measured by radioimmunoassay. In contrast to the normal stress-induced decrements in GH levels observed in controls, this response was blocked and stress caused a paradoxical increase (P less than 0.01) in GH levels in telencephalon-ablated rats. These results suggested that the telencephalon is essential for stress-induced inhibition of GH secretion. Therefore, the several components of the telencephalon were ablated individually in additional experiments to determine the structure(s) involved. Placement of large, bilateral lesions in the amygdala or complete removal by suction of hippocampus, septum or striatum did not interfere with the normal GH response to stress at 24 h after surgery. However, removal of the preoptic region duplicated the effects of complete telencephalon ablation i.e., the normal stress-induced decrement was not only blocked but also reversed. These findings suggest that the preoptic region is essential for mediating inhibition of GH secretion in response to immobilization-blood withdrawal stress and that other telencephalic structures are not essential for this neuroendocrine response.", "contents": "Extrahypothalamic control of stress-induced inhibition of growth hormone secretion in the rat. The aim of these experiments was to determine whether the telencephalon is essential for stress-induced suppression of plasma GH levels and, if so, which telencephalic structures are involved. In the first study, the telencephalon was completely removed from adult female rats by suction; intact and sham-operated rats served as controls. At 24 h after surgery, blood samples were collected at the initiation of and 15 min after a 3-min immobilization-blood withdrawal stress procedure and used for determining non-stress and stress plasma levels of GH, respectively. GH was measured by radioimmunoassay. In contrast to the normal stress-induced decrements in GH levels observed in controls, this response was blocked and stress caused a paradoxical increase (P less than 0.01) in GH levels in telencephalon-ablated rats. These results suggested that the telencephalon is essential for stress-induced inhibition of GH secretion. Therefore, the several components of the telencephalon were ablated individually in additional experiments to determine the structure(s) involved. Placement of large, bilateral lesions in the amygdala or complete removal by suction of hippocampus, septum or striatum did not interfere with the normal GH response to stress at 24 h after surgery. However, removal of the preoptic region duplicated the effects of complete telencephalon ablation i.e., the normal stress-induced decrement was not only blocked but also reversed. These findings suggest that the preoptic region is essential for mediating inhibition of GH secretion in response to immobilization-blood withdrawal stress and that other telencephalic structures are not essential for this neuroendocrine response.", "PMID": 976197}
{"id": "pubmed23n0032_10578", "title": "Compensatory thyroid hypertrophy after hemithyroidectomy in rats.", "content": "Thyroid enlargement occurs in association with a variety of circumstances characterized by an impaired capacity of the gland to secrete adequate amounts of hormone. To elucidate the factors responsible for such compensatory thyroid growth, particularly the role of TSH, we have observed the response of the serum TSH, T3 and T4 concentrations following hemithyroidectomy in the rat, and have attempted to correlate changes in these functions with changes in the weight and histology of the thyroid remnant. Hemithyroidectomy was performed in male Sprague-Dawley rats weighing 150 to 370 g, sham-operated animals serving as controls. As compared to findings in sham-operated animals, serum T4 concentrations declined promptly after hemithyroidectomy. In Experiment I serum T4 concentrations remained low for about 10 days and then returned to initial values. In Experiment II serum T4 concentrations remained lower than initial T4 values or values found in sham-operated animals until 34 days after hemithyroidectomy. Serum T3 concentrations were not significantly altered after hemithyroidectomy in either group but tended to be lower in the hemithyroidectomized animals. Serum TSH concentrations increased within 3 days after hemithyroidectomy and, for as long as 21 weeks, remained at values higher than those present preoperatively or those seen in sham-operated animals. Thyroid lobe weight increased following removal of the contralateral lobe and this increase was also sustained throughout the duration of the experiments. Biochemical and histological observations indicated that enlargement of the residual lobe was due to hypertrophy rather than hyperplasia.", "contents": "Compensatory thyroid hypertrophy after hemithyroidectomy in rats. Thyroid enlargement occurs in association with a variety of circumstances characterized by an impaired capacity of the gland to secrete adequate amounts of hormone. To elucidate the factors responsible for such compensatory thyroid growth, particularly the role of TSH, we have observed the response of the serum TSH, T3 and T4 concentrations following hemithyroidectomy in the rat, and have attempted to correlate changes in these functions with changes in the weight and histology of the thyroid remnant. Hemithyroidectomy was performed in male Sprague-Dawley rats weighing 150 to 370 g, sham-operated animals serving as controls. As compared to findings in sham-operated animals, serum T4 concentrations declined promptly after hemithyroidectomy. In Experiment I serum T4 concentrations remained low for about 10 days and then returned to initial values. In Experiment II serum T4 concentrations remained lower than initial T4 values or values found in sham-operated animals until 34 days after hemithyroidectomy. Serum T3 concentrations were not significantly altered after hemithyroidectomy in either group but tended to be lower in the hemithyroidectomized animals. Serum TSH concentrations increased within 3 days after hemithyroidectomy and, for as long as 21 weeks, remained at values higher than those present preoperatively or those seen in sham-operated animals. Thyroid lobe weight increased following removal of the contralateral lobe and this increase was also sustained throughout the duration of the experiments. Biochemical and histological observations indicated that enlargement of the residual lobe was due to hypertrophy rather than hyperplasia.", "PMID": 976198}
{"id": "pubmed23n0032_10579", "title": "Correlation between sexual steroid effect and activity changes and isoenzyme distribution of lactate dehydrogenase (EC 1.1.1.27) in rat anterior pituitary.", "content": "Sexual steroid feed-back is a fundamental regulating factor oification of pituitary metabolism in rat. Oestrogen synthesis and/or activity of M subunif testosterone, in males testosterone treatment as well as lack of oestrogen result in characteristic pituitary LDH activity and isoenzyme distribution. Physiological doses of sexual steroids affect, directly or indirectly, pituitary LDH activity, oxydative metabolism, and imply different biochemical bases for the polypeptide hormone production in the anterior pituitary gland.", "contents": "Correlation between sexual steroid effect and activity changes and isoenzyme distribution of lactate dehydrogenase (EC 1.1.1.27) in rat anterior pituitary. Sexual steroid feed-back is a fundamental regulating factor oification of pituitary metabolism in rat. Oestrogen synthesis and/or activity of M subunif testosterone, in males testosterone treatment as well as lack of oestrogen result in characteristic pituitary LDH activity and isoenzyme distribution. Physiological doses of sexual steroids affect, directly or indirectly, pituitary LDH activity, oxydative metabolism, and imply different biochemical bases for the polypeptide hormone production in the anterior pituitary gland.", "PMID": 976199}
{"id": "pubmed23n0032_10580", "title": "Sexual difference in pituitary pyruvic acid content, as well as of lactic acid/pyruvic acid quotient.", "content": "The lactic acid/pyruvic acid quotient of rat anterior pituitary varies betweeen 30 and 80. The dominance of lactic acid is in accordance with the earlier observation that pituitary lactate dehydrogenase isoenzymes in rat are made up, in majority, of M subunits. The pituitary lactic acid concentration in female and male animals shows no appreciable difference and does not change 2 and 4 weeks after castration. In males the pituitary pyruvic acid concentration was almost double of that in females. Pyruvic acid content increases after ovariectomy and decreases after orchidectomy. The sexual differences having been observed in lactic acid/pyruvic acid quotient are in correlation with the discrepancy of pyruvic acid concentration.", "contents": "Sexual difference in pituitary pyruvic acid content, as well as of lactic acid/pyruvic acid quotient. The lactic acid/pyruvic acid quotient of rat anterior pituitary varies betweeen 30 and 80. The dominance of lactic acid is in accordance with the earlier observation that pituitary lactate dehydrogenase isoenzymes in rat are made up, in majority, of M subunits. The pituitary lactic acid concentration in female and male animals shows no appreciable difference and does not change 2 and 4 weeks after castration. In males the pituitary pyruvic acid concentration was almost double of that in females. Pyruvic acid content increases after ovariectomy and decreases after orchidectomy. The sexual differences having been observed in lactic acid/pyruvic acid quotient are in correlation with the discrepancy of pyruvic acid concentration.", "PMID": 976201}
{"id": "pubmed23n0032_10581", "title": "[Metabolism of sulfate and phosphate conjugates of 19-nortestosterone enantiomers].", "content": "The sulfate and phosphate conjugates of 19-nortestosterone (17beta-hydroxy-estr-4-ene-3-one) and ent-19-nortestosterone (ent-17beta-hydroxy-estr-4-ene-3-one) have been synthesized. The sodium salts of the conjugates were i.v. infused to 2 women. On days 1--4 following application, the 19-nortestosterone and 17-ketosteroid excretion in urine as well as the 19-nortestosterone conjugation have been estimated. The 19-nortestosterone enantiomers were excreted in nearly the same amounts. In comparison to testosterone, the excretion of unchanged steroid (20--50%) is significantly higher. The sulfate conjugates of the enantiomers are not hydrolyzed in vivo, whereas the phosphate conjugates are completely splitted. Hydrolysis of ent-nortestosterone phosphate takes place obviously more slowly. Urinary 17-ketosteroids are significantly increased only following infusion of 19-nortestosterone phosphate.", "contents": "[Metabolism of sulfate and phosphate conjugates of 19-nortestosterone enantiomers]. The sulfate and phosphate conjugates of 19-nortestosterone (17beta-hydroxy-estr-4-ene-3-one) and ent-19-nortestosterone (ent-17beta-hydroxy-estr-4-ene-3-one) have been synthesized. The sodium salts of the conjugates were i.v. infused to 2 women. On days 1--4 following application, the 19-nortestosterone and 17-ketosteroid excretion in urine as well as the 19-nortestosterone conjugation have been estimated. The 19-nortestosterone enantiomers were excreted in nearly the same amounts. In comparison to testosterone, the excretion of unchanged steroid (20--50%) is significantly higher. The sulfate conjugates of the enantiomers are not hydrolyzed in vivo, whereas the phosphate conjugates are completely splitted. Hydrolysis of ent-nortestosterone phosphate takes place obviously more slowly. Urinary 17-ketosteroids are significantly increased only following infusion of 19-nortestosterone phosphate.", "PMID": 976202}
{"id": "pubmed23n0032_10582", "title": "Structural changes in the medial and central amygdala of the male rat, following neonatal castration and androgen treatment.", "content": "In contrast to the lateral amygdalar region, in the medial and central parts of the amygdala a significant female-like increase of neuronal nuclear volumes was found in adult male rats orchidectomized on the 1st day of life. These permanent structural changes of the medial and central amygdala could be prevented by androgen administration on the 3rd day of life.", "contents": "Structural changes in the medial and central amygdala of the male rat, following neonatal castration and androgen treatment. In contrast to the lateral amygdalar region, in the medial and central parts of the amygdala a significant female-like increase of neuronal nuclear volumes was found in adult male rats orchidectomized on the 1st day of life. These permanent structural changes of the medial and central amygdala could be prevented by androgen administration on the 3rd day of life.", "PMID": 976203}
{"id": "pubmed23n0032_10583", "title": "[Endocrinologic and pharmacologic-toxicologic findings with ethinyl-estradiol sulfonate. 8th communication. Complement and lysozyme in the serum of beagles after 6 months application of ethinylestradiol sulfonate].", "content": "In course of studies for the chronic toxicity of estrogens after oral application two parameters of the unspecific resistance - complement (CH50U and CB50U) and lysozyme - in sera of 30 beagle-dogs were studied. The doses of the daily (over 6 months) applicated hormones were 1,0 mg/kg ethinyl\u00f6stradiol and 1,0, 0,1 and 0,01 mg/kg ethinyl\u00f6stradiolsulfonate (depotform). In the majority of all measurements no significant variations of serum complement and -lysozyme could be detected, however after application of high doses of the hormone ethinyl\u00f6stradiol a little reduction of complement and significant elevations of lysozyme were observed.", "contents": "[Endocrinologic and pharmacologic-toxicologic findings with ethinyl-estradiol sulfonate. 8th communication. Complement and lysozyme in the serum of beagles after 6 months application of ethinylestradiol sulfonate]. In course of studies for the chronic toxicity of estrogens after oral application two parameters of the unspecific resistance - complement (CH50U and CB50U) and lysozyme - in sera of 30 beagle-dogs were studied. The doses of the daily (over 6 months) applicated hormones were 1,0 mg/kg ethinyl\u00f6stradiol and 1,0, 0,1 and 0,01 mg/kg ethinyl\u00f6stradiolsulfonate (depotform). In the majority of all measurements no significant variations of serum complement and -lysozyme could be detected, however after application of high doses of the hormone ethinyl\u00f6stradiol a little reduction of complement and significant elevations of lysozyme were observed.", "PMID": 976204}
{"id": "pubmed23n0032_10584", "title": "Thyroid testis inter-relationship in Indian palm squirrel, Funambulus pennanti Wroughton.", "content": "The present work attempts to find out a relationship between testicular functions and thyroid activity in a seasonal breeder, Indian palm squirrel, Funambulus pennanti Wroughton. In addition to radioiodine studies used for assessing thyroid status during different months in the year, biochemical estimations and histological studies have also been undertaken with a view to determine thyroid and testicular functions. Testicular recrudescence in Indian palm squirrel starts in January and peak activity is reached during April and May. June marks the beginning of testicular regression and the maximal regression is reached in October. Fluctuations in thyroid function are clearly manifested by variations observed in radioiodine uptake during different phases of reproductive cycle. Cytoarchitecture of thyroid also changes somewhat during different periods of the year and these changes are in accordance with the results obtained from radioiodine uptake studies. The present investigation suggests that the thyroid has a definite and significant role to play during the period of testicular recrudescence and regression in seasonal breeder Funambulus pennanti and it seems to help in tapering off sexual arousal and regression rather than sudden switching on and off of the reproductive cycle. In addition, it seems to facilitate peak testicular activity. However, mechanisms whereby thyroid influences various testicular functions seem to involve a complex series of interactions.", "contents": "Thyroid testis inter-relationship in Indian palm squirrel, Funambulus pennanti Wroughton. The present work attempts to find out a relationship between testicular functions and thyroid activity in a seasonal breeder, Indian palm squirrel, Funambulus pennanti Wroughton. In addition to radioiodine studies used for assessing thyroid status during different months in the year, biochemical estimations and histological studies have also been undertaken with a view to determine thyroid and testicular functions. Testicular recrudescence in Indian palm squirrel starts in January and peak activity is reached during April and May. June marks the beginning of testicular regression and the maximal regression is reached in October. Fluctuations in thyroid function are clearly manifested by variations observed in radioiodine uptake during different phases of reproductive cycle. Cytoarchitecture of thyroid also changes somewhat during different periods of the year and these changes are in accordance with the results obtained from radioiodine uptake studies. The present investigation suggests that the thyroid has a definite and significant role to play during the period of testicular recrudescence and regression in seasonal breeder Funambulus pennanti and it seems to help in tapering off sexual arousal and regression rather than sudden switching on and off of the reproductive cycle. In addition, it seems to facilitate peak testicular activity. However, mechanisms whereby thyroid influences various testicular functions seem to involve a complex series of interactions.", "PMID": 976205}
{"id": "pubmed23n0032_10585", "title": "The response of thyroid C-cell system in rat to long-term hypercalcemia.", "content": "Long-term hypercalcemia induced in rats by administration of vitamine D3 and CaCl2 for 60 days resulted in strong hyperplasia and hypertrophy of C-cells. The extent of hyperplasia varied greatly in individual animals. Histochemical reactions, especially the masked metachromasy with toluidine blue, demonstrated cell groups in which no reaction was observed beside those exhibiting a very strong reaction. Impregnation with silver according to Cajal showed a diminished number of argyrophilic granules in the C-cells, which had undergone hyperplasia. The reactions for non-specific esterases and cholinesterases were similar both in the experimental animals and in the controls. An enlargement of the C-cell nuclei in the experimental group was also pronounced. The total serum calcium level was only slightly increased in this group. It is concluded that the results of staining and the enlargement in nuclear volume of C-cells reflect the increased activity of these cells. Hyperplasia of the C-cells may represent a type of adaptation of the endocrine system in order to maintain calcium homeostasis.", "contents": "The response of thyroid C-cell system in rat to long-term hypercalcemia. Long-term hypercalcemia induced in rats by administration of vitamine D3 and CaCl2 for 60 days resulted in strong hyperplasia and hypertrophy of C-cells. The extent of hyperplasia varied greatly in individual animals. Histochemical reactions, especially the masked metachromasy with toluidine blue, demonstrated cell groups in which no reaction was observed beside those exhibiting a very strong reaction. Impregnation with silver according to Cajal showed a diminished number of argyrophilic granules in the C-cells, which had undergone hyperplasia. The reactions for non-specific esterases and cholinesterases were similar both in the experimental animals and in the controls. An enlargement of the C-cell nuclei in the experimental group was also pronounced. The total serum calcium level was only slightly increased in this group. It is concluded that the results of staining and the enlargement in nuclear volume of C-cells reflect the increased activity of these cells. Hyperplasia of the C-cells may represent a type of adaptation of the endocrine system in order to maintain calcium homeostasis.", "PMID": 976206}
{"id": "pubmed23n0032_10586", "title": "Decreased antidiuretic response in diabetes insipidus during prolonged treatment with carbamazepine presumably due to enzym-induction.", "content": "Carbamazepine (CA)-induced antidiuresis and fluorescence in urinary cortisol assay was compared during short-term and prolonged treatment in 3 patients with pituitary diabetes insipidus. The fluorescence induced by excreted CA-metabolite(s) (probably CA-10-11 epoxide and its derivatives) was used for the rough clinical estimation of the rate of metabolic elimination of the drug. A marked decrease was observed both in the antidiuretic response induced by CA and excretion of CA metabolite(s) during prolonged treatment - with a fixed dose of CA. These findings suggest that diminishing antidiuretic effectiveness might be due to auto-induction of CA. Auto-induction of CA may explain the delay in improvement of renal concentrating operation in patients with DI during long-term treatment with fixed doses of CA. Another clinical consequence of this mechanism may be the prevention of the development of water intoxication in neuro-psychiatric patients undergoing chronic treatment with CA.", "contents": "Decreased antidiuretic response in diabetes insipidus during prolonged treatment with carbamazepine presumably due to enzym-induction. Carbamazepine (CA)-induced antidiuresis and fluorescence in urinary cortisol assay was compared during short-term and prolonged treatment in 3 patients with pituitary diabetes insipidus. The fluorescence induced by excreted CA-metabolite(s) (probably CA-10-11 epoxide and its derivatives) was used for the rough clinical estimation of the rate of metabolic elimination of the drug. A marked decrease was observed both in the antidiuretic response induced by CA and excretion of CA metabolite(s) during prolonged treatment - with a fixed dose of CA. These findings suggest that diminishing antidiuretic effectiveness might be due to auto-induction of CA. Auto-induction of CA may explain the delay in improvement of renal concentrating operation in patients with DI during long-term treatment with fixed doses of CA. Another clinical consequence of this mechanism may be the prevention of the development of water intoxication in neuro-psychiatric patients undergoing chronic treatment with CA.", "PMID": 976207}
{"id": "pubmed23n0032_10587", "title": "Relationship between plasma renin activity and urinary catecholamines in various types of hypertension.", "content": "The urinary excretion of free noradrenaline (NA), adrenaline (A), dopamine (DA), the DA/NA ratio in the urine, plasma renin activity (PRA) and their mutual relationship were investigated in 71 patients suffering from different types of arterial hypertension. In spite of the fact that the mean values of excreted catecholamines, with the exception of pheochromocytoma, lie within the range of values found in healthy controls, certain differences were found in spectrum of excreted catecholamines. In patients with labile, malignant and renovascular hypertension and in pheochromcytoma the higher mean excretion of NA and the low DA/NA ratio was accompanied by the higher PRA in comparison with fixed benign essential hypertension. On the other hand, in hypertension with low PRA (essential hypertension with suppressed renin and Conn's syndrome) a low excretion of NA and high DA/NA ratio was found. There was a significant, if not even very close negative correlation between the PRA and DA/NA ratios both in recumbent and upright position. The rise of PRA on standing up was followed by an increased excretion of NA while the excretion of DA did not change or decreased. Hence the DA/NA ratio when standing up showed a decreasing tendency as compared with values when lying down. Application of the beta-blocker Inderal decreased the PRA and the blood pressure not only in juvenile hypertensive patients with hyperkinetic circulation but also in the early phases of renovascular hypertension. It thus appears that endogenous catecholamines, first of all the ratio between the renin-inhibiting DA and the renin-stimulating NA, participate as one of several factors in the regulation of secretion and of the plasma levels of renin not only in juvenile hypertensive patients with hyperkinetic circulation but also in other types of hypertension.", "contents": "Relationship between plasma renin activity and urinary catecholamines in various types of hypertension. The urinary excretion of free noradrenaline (NA), adrenaline (A), dopamine (DA), the DA/NA ratio in the urine, plasma renin activity (PRA) and their mutual relationship were investigated in 71 patients suffering from different types of arterial hypertension. In spite of the fact that the mean values of excreted catecholamines, with the exception of pheochromocytoma, lie within the range of values found in healthy controls, certain differences were found in spectrum of excreted catecholamines. In patients with labile, malignant and renovascular hypertension and in pheochromcytoma the higher mean excretion of NA and the low DA/NA ratio was accompanied by the higher PRA in comparison with fixed benign essential hypertension. On the other hand, in hypertension with low PRA (essential hypertension with suppressed renin and Conn's syndrome) a low excretion of NA and high DA/NA ratio was found. There was a significant, if not even very close negative correlation between the PRA and DA/NA ratios both in recumbent and upright position. The rise of PRA on standing up was followed by an increased excretion of NA while the excretion of DA did not change or decreased. Hence the DA/NA ratio when standing up showed a decreasing tendency as compared with values when lying down. Application of the beta-blocker Inderal decreased the PRA and the blood pressure not only in juvenile hypertensive patients with hyperkinetic circulation but also in the early phases of renovascular hypertension. It thus appears that endogenous catecholamines, first of all the ratio between the renin-inhibiting DA and the renin-stimulating NA, participate as one of several factors in the regulation of secretion and of the plasma levels of renin not only in juvenile hypertensive patients with hyperkinetic circulation but also in other types of hypertension.", "PMID": 976208}
{"id": "pubmed23n0032_10588", "title": "Diabetes mellitus and osteoporosis.", "content": "In 428 non selected diabetics, the authors determined the metacarpus index of Barnett-Nordin. Upon comparing the data with those of the control group it was shown that opposite to most references one has not to reckon in diabetes with the development of porosis and also that the decrease of the calcium content concomitant with age, respectively, does not significantly differ from that of the control group. Spontaneous vertebral compression occurred more rarely in diabetics than would have been expected on the basis of the literary data. The relation was examined between the type of diabetes, the time of its persisting and osteoporosis. The difference in the observations--compared to the previous ones - is, by the authors, assigned to the fact that nowadays no such lasting and severe metabolic disturbances develop in diabetics that would lead to rare-faction of the bony system and also that hormonal factors can become effective against the development of osteoporosis.", "contents": "Diabetes mellitus and osteoporosis. In 428 non selected diabetics, the authors determined the metacarpus index of Barnett-Nordin. Upon comparing the data with those of the control group it was shown that opposite to most references one has not to reckon in diabetes with the development of porosis and also that the decrease of the calcium content concomitant with age, respectively, does not significantly differ from that of the control group. Spontaneous vertebral compression occurred more rarely in diabetics than would have been expected on the basis of the literary data. The relation was examined between the type of diabetes, the time of its persisting and osteoporosis. The difference in the observations--compared to the previous ones - is, by the authors, assigned to the fact that nowadays no such lasting and severe metabolic disturbances develop in diabetics that would lead to rare-faction of the bony system and also that hormonal factors can become effective against the development of osteoporosis.", "PMID": 976209}
{"id": "pubmed23n0032_10589", "title": "Clinical and metabolic studies in children of diabetic-mothers.", "content": "Children of diabetic mothers are a high-risk group. The perinatal mortality and the malformation frequency of the newborns as well as the psychosomatic, somatic and speech development during childhood until the age of 15 were statistically significantly influenced by quality of metabolic compensation, toxaemia and urinary tract infections during pregnancy. To evaluate the diabetogenic risk in children of diabetic mothers metabolic follow-up studies were performed. A statistically significant insulin-high-response without disturbed carbohydrate tolerance was seen in 25% after intravenous glucose infusion and in 28% after oral glucose load. 17% exhibited a paradoxical growth hormone reaction, while the intravenous glucose tolerance and the insulin secretion was in the normal range. Both symptoms could be early diabetic signs in children of diabetic mothers. The oGTT had pathological results in 18% and the glucose infusion test in 8%.", "contents": "Clinical and metabolic studies in children of diabetic-mothers. Children of diabetic mothers are a high-risk group. The perinatal mortality and the malformation frequency of the newborns as well as the psychosomatic, somatic and speech development during childhood until the age of 15 were statistically significantly influenced by quality of metabolic compensation, toxaemia and urinary tract infections during pregnancy. To evaluate the diabetogenic risk in children of diabetic mothers metabolic follow-up studies were performed. A statistically significant insulin-high-response without disturbed carbohydrate tolerance was seen in 25% after intravenous glucose infusion and in 28% after oral glucose load. 17% exhibited a paradoxical growth hormone reaction, while the intravenous glucose tolerance and the insulin secretion was in the normal range. Both symptoms could be early diabetic signs in children of diabetic mothers. The oGTT had pathological results in 18% and the glucose infusion test in 8%.", "PMID": 976210}
{"id": "pubmed23n0032_10590", "title": "Liver phospholipid distribution in hypophysectomized catfish, Clarias batrachus and Heteropneustes fossilis.", "content": "The percentage of certain phospholipid fractions such as phosphatylcholine, phosphatidylserine and phosphatidylinositol was more in the liver of Clarias batrachus than that of the corresponding fractions in Heteropneustes fossilis. Hypophysectomy in both the species resulted in an increase in phosphatidylcholine, but reduction in other fractions. The phosphatidylinositol however, remained unchanged in C. batrachus, but it disappeared in H. fossilis as a result of hypophysectomy.", "contents": "Liver phospholipid distribution in hypophysectomized catfish, Clarias batrachus and Heteropneustes fossilis. The percentage of certain phospholipid fractions such as phosphatylcholine, phosphatidylserine and phosphatidylinositol was more in the liver of Clarias batrachus than that of the corresponding fractions in Heteropneustes fossilis. Hypophysectomy in both the species resulted in an increase in phosphatidylcholine, but reduction in other fractions. The phosphatidylinositol however, remained unchanged in C. batrachus, but it disappeared in H. fossilis as a result of hypophysectomy.", "PMID": 976211}
{"id": "pubmed23n0032_10591", "title": "Insulin resistant diabetes mellitus without the presence of insulin antibodies. A case report.", "content": "A case of a 26-year old woman suffering from an insulin resistant diabetes mellitus for 14 years is described. Acanthosis nigricans was diagnosed in the patient's second year and the syndrome of Stein-Leventhal at the age of 15. Diabetes could not be properly controlled either with the daily dosis of insulin as high as 1140 U or with peroral tolbutamide. Fasting serum IRI concentrations were elevated, the secretoric response to the stimulation by glucose or tolbutamide was substantial and protracted. The hypoglycemic response to the i.v. application of commercial insulin was insignificant. Serum growth hormone levels were normal. No presence of insulin antibodies in the serum was detected.", "contents": "Insulin resistant diabetes mellitus without the presence of insulin antibodies. A case report. A case of a 26-year old woman suffering from an insulin resistant diabetes mellitus for 14 years is described. Acanthosis nigricans was diagnosed in the patient's second year and the syndrome of Stein-Leventhal at the age of 15. Diabetes could not be properly controlled either with the daily dosis of insulin as high as 1140 U or with peroral tolbutamide. Fasting serum IRI concentrations were elevated, the secretoric response to the stimulation by glucose or tolbutamide was substantial and protracted. The hypoglycemic response to the i.v. application of commercial insulin was insignificant. Serum growth hormone levels were normal. No presence of insulin antibodies in the serum was detected.", "PMID": 976212}
{"id": "pubmed23n0032_10592", "title": "Opposite effects of spironolactone and amiloride on potassium excretion in a patient with ascites due to liver cirrhosis.", "content": "Potassium excretion was enhanced by spironolactone and it was inhibited by amiloride in a patient with ascites. One drug's effect was counteracted by the other when they were used in combination. Individual variation in the sites of action of antikaluretic drugs might be a possible explanation for the unpredictable changes in potassium excretion induced by these agents.", "contents": "Opposite effects of spironolactone and amiloride on potassium excretion in a patient with ascites due to liver cirrhosis. Potassium excretion was enhanced by spironolactone and it was inhibited by amiloride in a patient with ascites. One drug's effect was counteracted by the other when they were used in combination. Individual variation in the sites of action of antikaluretic drugs might be a possible explanation for the unpredictable changes in potassium excretion induced by these agents.", "PMID": 976213}
{"id": "pubmed23n0032_10593", "title": "Prevalence of epilepsy in children of Multnomah County, Oregon.", "content": "The prevalence of epilepsy among children in the third grade in Multnomah County, Oregon, was investigated by use of a questionnaire similar to that used by Rose et al. (1973). Of the 7,039 potential respondents, 5,317 (75.5%) responded to the questionnaire. A stratified random sample of 161 children was drawn from the respondents, and of these 129 (80%) underwent physical and EEG examinations. This replication of the study by Rose et al. (1973) yielded prevalence estimates which were somewhat lower than those reported by Rose et al. for Washington County, Maryland. Our findings suggest that the questionnaire method of estimating the prevalence of epilepsy has promise; further investigation of the method is in progress.", "contents": "Prevalence of epilepsy in children of Multnomah County, Oregon. The prevalence of epilepsy among children in the third grade in Multnomah County, Oregon, was investigated by use of a questionnaire similar to that used by Rose et al. (1973). Of the 7,039 potential respondents, 5,317 (75.5%) responded to the questionnaire. A stratified random sample of 161 children was drawn from the respondents, and of these 129 (80%) underwent physical and EEG examinations. This replication of the study by Rose et al. (1973) yielded prevalence estimates which were somewhat lower than those reported by Rose et al. for Washington County, Maryland. Our findings suggest that the questionnaire method of estimating the prevalence of epilepsy has promise; further investigation of the method is in progress.", "PMID": 976215}
{"id": "pubmed23n0032_10594", "title": "EEG and clinical studies of the development of alumina cream epileptic focus in split-brain cats.", "content": "Alumina cream epileptic focus was established in the right sensorimotor cortex in 20 split-brain cats (partial or complete). EEG and behavioral observations were made in a period ranging from 24 to 836 days. Four types of EEG changes after alumina cream injection were differentiated. These types could be related to the direct effects of brain damage and to development of epilepsy. Spikes and sharp waves and paroxysmal discharges (focal and multifocal) were observed in about 60% of the cats. Clinical seizures developed in about the same percentage of the animals. These values are below those reported for cats with intact interhemispheric commissures. Diphenylhydantoin (DPH) was given orally in a daily dose of up to 15 mg/kg body weight in 9 animals with developed epileptic EEG activity. Five of them had epileptic seizures. DPH was introduced not earlier than 1.5 months after intracortical alumina cream injection. The plasma level of DPH varied between 7-20 mug/ml. This dose produced chronic symptoms of intoxication. Neither EEG changes nor clinical seizures were entirely controlled by this drug. Additional doses of Relanium (diazepam), and phenobarbital were necessary to stop generalized seizures or status epilepticus.", "contents": "EEG and clinical studies of the development of alumina cream epileptic focus in split-brain cats. Alumina cream epileptic focus was established in the right sensorimotor cortex in 20 split-brain cats (partial or complete). EEG and behavioral observations were made in a period ranging from 24 to 836 days. Four types of EEG changes after alumina cream injection were differentiated. These types could be related to the direct effects of brain damage and to development of epilepsy. Spikes and sharp waves and paroxysmal discharges (focal and multifocal) were observed in about 60% of the cats. Clinical seizures developed in about the same percentage of the animals. These values are below those reported for cats with intact interhemispheric commissures. Diphenylhydantoin (DPH) was given orally in a daily dose of up to 15 mg/kg body weight in 9 animals with developed epileptic EEG activity. Five of them had epileptic seizures. DPH was introduced not earlier than 1.5 months after intracortical alumina cream injection. The plasma level of DPH varied between 7-20 mug/ml. This dose produced chronic symptoms of intoxication. Neither EEG changes nor clinical seizures were entirely controlled by this drug. Additional doses of Relanium (diazepam), and phenobarbital were necessary to stop generalized seizures or status epilepticus.", "PMID": 976216}
{"id": "pubmed23n0032_10595", "title": "Effects of drugs modifying brain levels of catecholamines on photically induced epilepsy in Papio papio.", "content": "The behavioral and electrographic effects of DL-amphetamine, disulfiram, FLA-63, and propranolol on the photically induced epilepsy of the Senegalese baboon were evaluated. Amphetamine produced somatic hypokinesia with enhancement of eye movements, diminution of spontaneous paroxysmal activity, and little change in photosensitivity. The acute effects of disulfiram and FLA-63 were poor, but the latter was lethal in the days following administration. Propranolol showed no consistent effects. Participation of catecholamine processes in this type of experimental reflex epilepsy are discussed.", "contents": "Effects of drugs modifying brain levels of catecholamines on photically induced epilepsy in Papio papio. The behavioral and electrographic effects of DL-amphetamine, disulfiram, FLA-63, and propranolol on the photically induced epilepsy of the Senegalese baboon were evaluated. Amphetamine produced somatic hypokinesia with enhancement of eye movements, diminution of spontaneous paroxysmal activity, and little change in photosensitivity. The acute effects of disulfiram and FLA-63 were poor, but the latter was lethal in the days following administration. Propranolol showed no consistent effects. Participation of catecholamine processes in this type of experimental reflex epilepsy are discussed.", "PMID": 976217}
{"id": "pubmed23n0032_10596", "title": "A teachers' questionnaire description of epileptic children.", "content": "A teachers' questionnaire was completed for 120 children from normal schools, half of whom suffered from epilepsy. The rate of perceived disturbance among the 30 epileptic children who, for a variety of reasons, had been admitted to a neuropsychiatric hospital was as high as that of a matched group of psychiatric inpatients. The other epileptic children were seen to exhibit significantly more signs of disturbance than their normal school controls with whom they had been matched for behavior. The two epileptic groups shared certain behavioral characteristics. These did not fit into typical \"neurotic\" or \"antisocial\" patterns and may relate to cerebral dysfunction.", "contents": "A teachers' questionnaire description of epileptic children. A teachers' questionnaire was completed for 120 children from normal schools, half of whom suffered from epilepsy. The rate of perceived disturbance among the 30 epileptic children who, for a variety of reasons, had been admitted to a neuropsychiatric hospital was as high as that of a matched group of psychiatric inpatients. The other epileptic children were seen to exhibit significantly more signs of disturbance than their normal school controls with whom they had been matched for behavior. The two epileptic groups shared certain behavioral characteristics. These did not fit into typical \"neurotic\" or \"antisocial\" patterns and may relate to cerebral dysfunction.", "PMID": 976218}
{"id": "pubmed23n0032_10597", "title": "Phenytoin-induced depression of salivary IgA and gingival hyperplasia.", "content": "IgA concentrations were determined in saliva from epileptic patients taking phenytoin and in saliva from healthy controls, by single radial immunodiffusion technique. Mean salivary IgA in epileptic children was 7.23 mg/ml; in healthy children, 16.44 mg/ml. Corresponding values for adults were 13.53 and 19.48 mg/ml, respectively. In 14 out of 84 samples, salivary IgA levels were too low for quantitative analysis. Salivary IgA levels were normal in untreated patients and fell during treatment with phenytoin. Gingival inflammation was commonly accompanied by an increase of salivary IgG and serum-derived IgA, whereas compensatory increase of IgM was infrequent. Phenytoin-induced deficiency of salivary IgA can result in increased susceptibility to gingival inflammation, which is considered one of the predisposing factors for subsequent development of gingival hyperplasia.", "contents": "Phenytoin-induced depression of salivary IgA and gingival hyperplasia. IgA concentrations were determined in saliva from epileptic patients taking phenytoin and in saliva from healthy controls, by single radial immunodiffusion technique. Mean salivary IgA in epileptic children was 7.23 mg/ml; in healthy children, 16.44 mg/ml. Corresponding values for adults were 13.53 and 19.48 mg/ml, respectively. In 14 out of 84 samples, salivary IgA levels were too low for quantitative analysis. Salivary IgA levels were normal in untreated patients and fell during treatment with phenytoin. Gingival inflammation was commonly accompanied by an increase of salivary IgG and serum-derived IgA, whereas compensatory increase of IgM was infrequent. Phenytoin-induced deficiency of salivary IgA can result in increased susceptibility to gingival inflammation, which is considered one of the predisposing factors for subsequent development of gingival hyperplasia.", "PMID": 976219}
{"id": "pubmed23n0032_10598", "title": "Differences between patients with temporal lobe seizures and those with other forms of epileptic attacks.", "content": "Seventy-eight patients with temporal lobe type seizures were precisely matched for age, sex, and Wechsler I.Q. levels with patients suffering from other seizure types. Statistically significant differences were established between the groups in regard to treatment response, etiologies, and behavioral variables. Further analysis of the data showed that the behavioral problems of the temporal lobe patients were present only in that group that suffered from more than one seizure type. That temporal lobe epilepsy group having only one seizure type differed from the controls with one seizure type in regard to etiological factors; they actually showed better performance on certain subscales of the WAIS, as well as better employment records. The clinical impression of the temporal lobe epilepsy patient usually presenting more difficulties than other patients with epilepsy results from the fact that the temporal lobe patients have, in the overwhelming majority of instances, more than one seizure type.", "contents": "Differences between patients with temporal lobe seizures and those with other forms of epileptic attacks. Seventy-eight patients with temporal lobe type seizures were precisely matched for age, sex, and Wechsler I.Q. levels with patients suffering from other seizure types. Statistically significant differences were established between the groups in regard to treatment response, etiologies, and behavioral variables. Further analysis of the data showed that the behavioral problems of the temporal lobe patients were present only in that group that suffered from more than one seizure type. That temporal lobe epilepsy group having only one seizure type differed from the controls with one seizure type in regard to etiological factors; they actually showed better performance on certain subscales of the WAIS, as well as better employment records. The clinical impression of the temporal lobe epilepsy patient usually presenting more difficulties than other patients with epilepsy results from the fact that the temporal lobe patients have, in the overwhelming majority of instances, more than one seizure type.", "PMID": 976220}
{"id": "pubmed23n0032_10599", "title": "Insect growth regulators and insect control: a critical appraisal.", "content": "Insect growth regulators (IGRs) of the juvenile hormone type alter physiological processes essential to insect development and appear to act specifically on insects. Three natural juvenile hormones have been found in insects but not in other organisms. Future use of antagonists or inhibitors of hormone synthesis may be technically possible as an advantageous extension of pest control by IGRs. A documented survey of the properties, metabolism, toxicology, and uses of the most commercially advanced chemical, methoprene, shows it to be environmentally acceptable and toxicologically innocuous. Derivation of its current use patterns is discussed and limitations on these are noted. Residue levels and their measurement in the ppb region have allowed exemption from the requirement of tolerances in the EPA registered use of methoprene for mosquito control. Tolerances for foods accompany its fully approved use for control of manure breeding flies through a cattle feed supplement. The human health effects of using this chemical appear to be purely beneficial, but further advances through new IGR chemicals appear unlikely without major changes in regulatory and legislative policy.", "contents": "Insect growth regulators and insect control: a critical appraisal. Insect growth regulators (IGRs) of the juvenile hormone type alter physiological processes essential to insect development and appear to act specifically on insects. Three natural juvenile hormones have been found in insects but not in other organisms. Future use of antagonists or inhibitors of hormone synthesis may be technically possible as an advantageous extension of pest control by IGRs. A documented survey of the properties, metabolism, toxicology, and uses of the most commercially advanced chemical, methoprene, shows it to be environmentally acceptable and toxicologically innocuous. Derivation of its current use patterns is discussed and limitations on these are noted. Residue levels and their measurement in the ppb region have allowed exemption from the requirement of tolerances in the EPA registered use of methoprene for mosquito control. Tolerances for foods accompany its fully approved use for control of manure breeding flies through a cattle feed supplement. The human health effects of using this chemical appear to be purely beneficial, but further advances through new IGR chemicals appear unlikely without major changes in regulatory and legislative policy.", "PMID": 976222}
{"id": "pubmed23n0032_10600", "title": "Public health advantages of biological insect controls.", "content": "Biological control is not new, it is simply newly appreciated. This renewed appreciation stems from the widespread insecticide treadmill which is largely a product of insecticide disruption of the balance of insect communities. Biological control is a natural phenomenon; the regulation of plant and animal numbers by natural enemies. In this broad sense, biological control is vital to public health because it keeps the myriad insect species from out-competing us. It also has direct public health advantages as where natural enemies are manipulated to control disease vectoring insects. Insecticide distruption of biological control by insecticides and the resulting pesticide treadmill have serious public health implications. One is the increased pesticide load in the environment. The other is the acceleration of pesticide resistance in disease vectoring insects. The treadmill and its associated hazards will not abate so long as chemical control dominates our pest management strategy.", "contents": "Public health advantages of biological insect controls. Biological control is not new, it is simply newly appreciated. This renewed appreciation stems from the widespread insecticide treadmill which is largely a product of insecticide disruption of the balance of insect communities. Biological control is a natural phenomenon; the regulation of plant and animal numbers by natural enemies. In this broad sense, biological control is vital to public health because it keeps the myriad insect species from out-competing us. It also has direct public health advantages as where natural enemies are manipulated to control disease vectoring insects. Insecticide distruption of biological control by insecticides and the resulting pesticide treadmill have serious public health implications. One is the increased pesticide load in the environment. The other is the acceleration of pesticide resistance in disease vectoring insects. The treadmill and its associated hazards will not abate so long as chemical control dominates our pest management strategy.", "PMID": 976223}
{"id": "pubmed23n0032_10601", "title": "Integrated pest management.", "content": "Integrated pest management, in which the conventional pesticides are augmented by one or more nonchemical control practices, has been receiving renewed interest. What is new in this revitalization of an old technique is the careful and more knowledgeable application of a variety of control techniques.", "contents": "Integrated pest management. Integrated pest management, in which the conventional pesticides are augmented by one or more nonchemical control practices, has been receiving renewed interest. What is new in this revitalization of an old technique is the careful and more knowledgeable application of a variety of control techniques.", "PMID": 976224}
{"id": "pubmed23n0032_10602", "title": "Health-related aspects of integrated pest management.", "content": "Basic concepts and philosophy of integrated pest management are presented in order to dispel several misconceptions and to provide the necessary background information for discussion of its relationship to the health-related effects of pesticide use. Implications for human health of current pesticide practices in Central America are examined to illustrate major problems associated with the injudicious use of insecticides, i.e., human pesticide poisonings, development of insect resistance, and persistence in the environment. Mitigation of these problems would ideally be achieved through the efforts and cooperation of a multidisciplinary team of scientists and technical people in the medical and agricultural sciences. The dilemma associated with the development of integrated pest control systems in developing countries is discussed. The FAO/UNEP Global Programme was reviewed.", "contents": "Health-related aspects of integrated pest management. Basic concepts and philosophy of integrated pest management are presented in order to dispel several misconceptions and to provide the necessary background information for discussion of its relationship to the health-related effects of pesticide use. Implications for human health of current pesticide practices in Central America are examined to illustrate major problems associated with the injudicious use of insecticides, i.e., human pesticide poisonings, development of insect resistance, and persistence in the environment. Mitigation of these problems would ideally be achieved through the efforts and cooperation of a multidisciplinary team of scientists and technical people in the medical and agricultural sciences. The dilemma associated with the development of integrated pest control systems in developing countries is discussed. The FAO/UNEP Global Programme was reviewed.", "PMID": 976225}
{"id": "pubmed23n0032_10603", "title": "Organotin compounds and their use for insect and mite control.", "content": "A brief overview of biological activity of organotin compounds is given, with emphasis on activity against plant-feeding arthropods. Plictran miticide, containing cyhexatin (tricyclohexylhydroxystannane), the first product to be fully commercialized for control of plant-feeding mites on a broad range of agricultural crops, is used as a model to indicate the chemistry and potential performance, phytotoxicity, residues, limitations on usage, environmental acceptability, and use patterns for products containing similar organotin compounds.", "contents": "Organotin compounds and their use for insect and mite control. A brief overview of biological activity of organotin compounds is given, with emphasis on activity against plant-feeding arthropods. Plictran miticide, containing cyhexatin (tricyclohexylhydroxystannane), the first product to be fully commercialized for control of plant-feeding mites on a broad range of agricultural crops, is used as a model to indicate the chemistry and potential performance, phytotoxicity, residues, limitations on usage, environmental acceptability, and use patterns for products containing similar organotin compounds.", "PMID": 976226}
{"id": "pubmed23n0032_10604", "title": "Biochemical and physiological effects of chlordimeform.", "content": "Chlordimeform is a relatively new acaricide/insecticide, whose mode of action we have investigated. It appears to interfere with amine-mediated control of nervous and endocrine systems in a variety of ways. Specifically, chlordimeform causes a build-up of the amines 5-hydroxytryptamine and to a lesser extent norepinephrine in the rat brain in vivo, antagonizes the in vivo action of reserpine in the rat (reserpine depletes amine stores in the CNS), inhibits monoamine oxidase from rat liver in vitro, and causes hypotension in rabbits. In the American cockroach it directly stimulates the heart in situ, acts synergistically with tryptamine in vivo, inhibits amine-N-acetyltransferase from cockroach head in vitro, causes accumulation of indolamines in cockroaches in vivo, and blocks the stimulation of adenylate cyclase by octopamine in the cockroach CNS in situ. It also inhibits tryptamine metabolism in whole mites in vitro.", "contents": "Biochemical and physiological effects of chlordimeform. Chlordimeform is a relatively new acaricide/insecticide, whose mode of action we have investigated. It appears to interfere with amine-mediated control of nervous and endocrine systems in a variety of ways. Specifically, chlordimeform causes a build-up of the amines 5-hydroxytryptamine and to a lesser extent norepinephrine in the rat brain in vivo, antagonizes the in vivo action of reserpine in the rat (reserpine depletes amine stores in the CNS), inhibits monoamine oxidase from rat liver in vitro, and causes hypotension in rabbits. In the American cockroach it directly stimulates the heart in situ, acts synergistically with tryptamine in vivo, inhibits amine-N-acetyltransferase from cockroach head in vitro, causes accumulation of indolamines in cockroaches in vivo, and blocks the stimulation of adenylate cyclase by octopamine in the cockroach CNS in situ. It also inhibits tryptamine metabolism in whole mites in vitro.", "PMID": 976227}
{"id": "pubmed23n0032_10605", "title": "The husbandry and veterinary care of wild horses in captivity.", "content": "A short account of the natural history of the wild Equidae is given as a background to their management in captivity. In general the approach to their husbandry and veterinary care is similar to that for the domestic horse. A mixture of etorphine and acepromazine has been found to be the most effective for handling and conventional methods of anaesthesia can be safely superimposed on these if required. The data collected at Whipsnade on the haematology and biochemistry of these species is summarised in tabular form and an analysis is included of the post-mortem examinations of 105 equine carcases from the Society's collection.", "contents": "The husbandry and veterinary care of wild horses in captivity. A short account of the natural history of the wild Equidae is given as a background to their management in captivity. In general the approach to their husbandry and veterinary care is similar to that for the domestic horse. A mixture of etorphine and acepromazine has been found to be the most effective for handling and conventional methods of anaesthesia can be safely superimposed on these if required. The data collected at Whipsnade on the haematology and biochemistry of these species is summarised in tabular form and an analysis is included of the post-mortem examinations of 105 equine carcases from the Society's collection.", "PMID": 976228}
{"id": "pubmed23n0032_10606", "title": "Percutaneous needle muscle biopsy in the horse.", "content": "The use of the technique of percutaneous needle biopsy in obtaining skeletal muscle samples in the horse is described. The biochemical, ultrastructural and histochemical investigations that can be carried out on this biopsy specimen are outlined. Analyses performed on the specimen may be used to obtain information on racing potential and state of fitness. These studies on normal horses will provide information for future investigations into the structural and biochemical alterations in muscle disorders in the equine.", "contents": "Percutaneous needle muscle biopsy in the horse. The use of the technique of percutaneous needle biopsy in obtaining skeletal muscle samples in the horse is described. The biochemical, ultrastructural and histochemical investigations that can be carried out on this biopsy specimen are outlined. Analyses performed on the specimen may be used to obtain information on racing potential and state of fitness. These studies on normal horses will provide information for future investigations into the structural and biochemical alterations in muscle disorders in the equine.", "PMID": 976229}
{"id": "pubmed23n0032_10607", "title": "Congenital deformities in two Clydesdale foals.", "content": "The deformities observed in 2 Clydesdale foals are described. Both had abnormal joint positions in the forelimbs and discrepancies in the symmetry of the vertebral column. The changes were only mild in one case but extreme in the other where it was accompanied by torticollis, scoliosis and vertebral fusion. A comparison is made with deformities described in the contracted foal syndrome and some of the developmental implications discussed.", "contents": "Congenital deformities in two Clydesdale foals. The deformities observed in 2 Clydesdale foals are described. Both had abnormal joint positions in the forelimbs and discrepancies in the symmetry of the vertebral column. The changes were only mild in one case but extreme in the other where it was accompanied by torticollis, scoliosis and vertebral fusion. A comparison is made with deformities described in the contracted foal syndrome and some of the developmental implications discussed.", "PMID": 976230}
{"id": "pubmed23n0032_10608", "title": "An investigation into the genetics of 'wobbler disease' in thoroughbred horses in Britain.", "content": "Sixty-seven TB horses in Britain suffering from clinical wobbler disease were divided into 5 groups, based on the presence or absence of pathological lesions and their nature. These groups were compared genetically with a control group of 67 TBs known not to exhibit signs of wobbling. Though analysed for both simple and complex modes of inheritance no evidence of a genetic basis was found in any group. The high breed incidence in TBs may be real or apparent. Considerably more male than female wobblers are reported. Other forms of possibly inherited ataxia in equidae are reviewed and reference is made to pathologically comparable conditions occurring in man and in the large breeds of dogs.", "contents": "An investigation into the genetics of 'wobbler disease' in thoroughbred horses in Britain. Sixty-seven TB horses in Britain suffering from clinical wobbler disease were divided into 5 groups, based on the presence or absence of pathological lesions and their nature. These groups were compared genetically with a control group of 67 TBs known not to exhibit signs of wobbling. Though analysed for both simple and complex modes of inheritance no evidence of a genetic basis was found in any group. The high breed incidence in TBs may be real or apparent. Considerably more male than female wobblers are reported. Other forms of possibly inherited ataxia in equidae are reviewed and reference is made to pathologically comparable conditions occurring in man and in the large breeds of dogs.", "PMID": 976231}
{"id": "pubmed23n0032_10609", "title": "Successful repair of a diaphragmatic hernia in a foal.", "content": "The diagnosis and successful surgical treatment of a diaphragmatic hernia in a 12 weeks old standardbred foal are described. The clinical syndrome in horses generally is contrasted with that seen in the foal described in this paper. Some of the factors contributing to the clinical signs are discussed.", "contents": "Successful repair of a diaphragmatic hernia in a foal. The diagnosis and successful surgical treatment of a diaphragmatic hernia in a 12 weeks old standardbred foal are described. The clinical syndrome in horses generally is contrasted with that seen in the foal described in this paper. Some of the factors contributing to the clinical signs are discussed.", "PMID": 976232}
{"id": "pubmed23n0032_10610", "title": "The use of Dopram as a respiratory stimulant following Immobilon in the pony.", "content": "The effects of the analeptic agent, Dopram (doxapram hydrochloride) were investigated in 2 ponies during Immobilon - induced neuroleptanalgesia. Although Dopram was demonstrated to exert a degree of respiratory stimulation, this was concluded to provide no overall advantage. The etorphine-induced hypoxic hypoxia was only partially reversed, and there was additional cardiovascular stimulation, in contrast to the previously reported tendency for arterial blood pressure to return towards conscious control values during the course of action of Immobilon.", "contents": "The use of Dopram as a respiratory stimulant following Immobilon in the pony. The effects of the analeptic agent, Dopram (doxapram hydrochloride) were investigated in 2 ponies during Immobilon - induced neuroleptanalgesia. Although Dopram was demonstrated to exert a degree of respiratory stimulation, this was concluded to provide no overall advantage. The etorphine-induced hypoxic hypoxia was only partially reversed, and there was additional cardiovascular stimulation, in contrast to the previously reported tendency for arterial blood pressure to return towards conscious control values during the course of action of Immobilon.", "PMID": 976233}
{"id": "pubmed23n0032_10611", "title": "An interventricular septal defect, pulmonary stenosis and bicuspid pulmonary valve in a Welsh pony foal.", "content": "A 6 month Welsh pony filly with an interventricular septal defect in conjunction with a pulmonary stenosis due to a bicuspid pulmonary valve is described. The animal had poor exercise tolerance, a loud pansystolic murmur and a precordial thrill. Blood pressure and oxygen tension values obtained during cardiac catheterization suggested the diagnosis which was confirmed at autopsy.", "contents": "An interventricular septal defect, pulmonary stenosis and bicuspid pulmonary valve in a Welsh pony foal. A 6 month Welsh pony filly with an interventricular septal defect in conjunction with a pulmonary stenosis due to a bicuspid pulmonary valve is described. The animal had poor exercise tolerance, a loud pansystolic murmur and a precordial thrill. Blood pressure and oxygen tension values obtained during cardiac catheterization suggested the diagnosis which was confirmed at autopsy.", "PMID": 976234}
{"id": "pubmed23n0032_10612", "title": "Succinate accumulation in man during exercise.", "content": "It has been demonstrated in several diving vertebrates that succinate, a component of the Krebs cycle, accumulates in blood during breath-hold dives. The production of succinate is thought to result from amino acid catabolism. Our purpose was to determine whether succinate accumulation occurs in man during muscular activity requiring anaerobic energy contribution. Experiments using an endurance athlete included apneic work on an underwater ergometer and treadmill running to exhaustion. During 1 min breath-hold \"dives\" in cold water while exercising at a work rate equivalent to 62% of VO2max, venous succinate increased from 42 mumoles/l (M X 10(-6)) at rest to 125 M X 10(-6). The treadmill run elicited VO2max and increased succinate from a similar resting value to 93 M X 10(-6). Increases in alanine, lactate, and pyruvate were observed for both types of exercise. The findings confirm that succinate accumulation also occurs in man. It was suggested that amino acid catabolism may provide a source of anaerobic energy production in addition to glycolysis. However, the importance of the proposed energy pathway remains to be quantified.", "contents": "Succinate accumulation in man during exercise. It has been demonstrated in several diving vertebrates that succinate, a component of the Krebs cycle, accumulates in blood during breath-hold dives. The production of succinate is thought to result from amino acid catabolism. Our purpose was to determine whether succinate accumulation occurs in man during muscular activity requiring anaerobic energy contribution. Experiments using an endurance athlete included apneic work on an underwater ergometer and treadmill running to exhaustion. During 1 min breath-hold \"dives\" in cold water while exercising at a work rate equivalent to 62% of VO2max, venous succinate increased from 42 mumoles/l (M X 10(-6)) at rest to 125 M X 10(-6). The treadmill run elicited VO2max and increased succinate from a similar resting value to 93 M X 10(-6). Increases in alanine, lactate, and pyruvate were observed for both types of exercise. The findings confirm that succinate accumulation also occurs in man. It was suggested that amino acid catabolism may provide a source of anaerobic energy production in addition to glycolysis. However, the importance of the proposed energy pathway remains to be quantified.", "PMID": 976251}
{"id": "pubmed23n0032_10613", "title": "Effects of an anabolic steroid and sprint training on selected histochemical and morphological observations in rat skeletal muscle types.", "content": "The effects on selected histochemical and morphological parameters of anabolic steroid administration and of high-intensity sprint running, separately, and in combination, were studied in young adult male rats. Dianabol (methandrostenolone) 1 mg/day for 8 weeks had no significant effects on phosphorylase or glycogen staining intensities and on fiber area in skeletal muscles of either trained or sedentary animals. The program of sprint training resulted in significantly decreased intensities of phosphorylase in all ten regions of the gastrocnemius, plantaris, and soleus muscles that were studied. Glycogen localization was significantly increased with training in five regions of the gastrocnemius and plantaris muscles which contained predominantly fast-switch fibers. No changes in fiber area occurred with the training program. We conclude from these results that (a) normal androgen levels in young, healthy male animals are sufficiently high so that the intake of large doses of anabolic steroid does not result in the stimulation of glycogen metabolism or hypertrophy of skeletal muscle; (b) the changes induced by high-intensity, short-duration sprint training suggest that the existing glycolytic capacity of muscle is adequate to supply the muscles energy needs even during the stress of very strenous exercise, and that more fast-twitch fibers were recruited by the exercise regimen than slow-twitch fibers.", "contents": "Effects of an anabolic steroid and sprint training on selected histochemical and morphological observations in rat skeletal muscle types. The effects on selected histochemical and morphological parameters of anabolic steroid administration and of high-intensity sprint running, separately, and in combination, were studied in young adult male rats. Dianabol (methandrostenolone) 1 mg/day for 8 weeks had no significant effects on phosphorylase or glycogen staining intensities and on fiber area in skeletal muscles of either trained or sedentary animals. The program of sprint training resulted in significantly decreased intensities of phosphorylase in all ten regions of the gastrocnemius, plantaris, and soleus muscles that were studied. Glycogen localization was significantly increased with training in five regions of the gastrocnemius and plantaris muscles which contained predominantly fast-switch fibers. No changes in fiber area occurred with the training program. We conclude from these results that (a) normal androgen levels in young, healthy male animals are sufficiently high so that the intake of large doses of anabolic steroid does not result in the stimulation of glycogen metabolism or hypertrophy of skeletal muscle; (b) the changes induced by high-intensity, short-duration sprint training suggest that the existing glycolytic capacity of muscle is adequate to supply the muscles energy needs even during the stress of very strenous exercise, and that more fast-twitch fibers were recruited by the exercise regimen than slow-twitch fibers.", "PMID": 976252}
{"id": "pubmed23n0032_10614", "title": "Metabolic changes during exercise testing of patients with ischaemic heart disease.", "content": "Nineteen patients who had recovered from a mild to moderately severe myocardial infraction 6--18 months previously were studied in order to investigate the relationship between the severity of myocardial insufficiency and metabolic changes developing during and after exercise. The patients were compared with six controls by means of a graded exercise test on a treadmill. Electrocardiographic records were made and blood pressure measured during and after the exercise and venous blood samples were taken for measurement of lactate, pyruvate, glycerol and free fatty acids. In five patients with moderate or advanced ischaemic electrocardiograph changes the blood lactate and pyruvate mean concentrations continued to rise up to a further 50% after they stopped exercising. The other patients, including some who developed symptoms suggestive of myocardial ischaemia, and all the controls, failed to show these marked increases in blood concentrations after the exercise. We conclude that the development of myocardial insufficiency during exercise is associated with marked increases in lactate and pyruvate concentrations in the peripheral blood particularly after the exercise has ceased.", "contents": "Metabolic changes during exercise testing of patients with ischaemic heart disease. Nineteen patients who had recovered from a mild to moderately severe myocardial infraction 6--18 months previously were studied in order to investigate the relationship between the severity of myocardial insufficiency and metabolic changes developing during and after exercise. The patients were compared with six controls by means of a graded exercise test on a treadmill. Electrocardiographic records were made and blood pressure measured during and after the exercise and venous blood samples were taken for measurement of lactate, pyruvate, glycerol and free fatty acids. In five patients with moderate or advanced ischaemic electrocardiograph changes the blood lactate and pyruvate mean concentrations continued to rise up to a further 50% after they stopped exercising. The other patients, including some who developed symptoms suggestive of myocardial ischaemia, and all the controls, failed to show these marked increases in blood concentrations after the exercise. We conclude that the development of myocardial insufficiency during exercise is associated with marked increases in lactate and pyruvate concentrations in the peripheral blood particularly after the exercise has ceased.", "PMID": 976253}
{"id": "pubmed23n0032_10615", "title": "Effect of physical fitness on vanillylmandelic acid excretion during immersion.", "content": "The effects of 4--6 h head-out immersion on excretion of vanillylmandelic acid (VMA), blood pressure and plasma volume were estimated in 8 endurance-trained (TR) and 8 untrained (UT) subjects. In the trained only a slight increase of VMA excretion occurred (4 h value: +2.7 +/- 10.9 ng/ml GFR), but there was a highly significant increase in the UT (+29.0 +/- 17.2 ng/ml GFR). VMA values during control experiments in supine position tended to decrease in both groups. Systolic and diastolic blood pressure fell by 20 mm Hg after beginning of immersion; in the UT plasma volume was reduced while it remained constant in TR. The results indicate that orthostatic intolerance (o.i.) after immersion is not effected by decreased sympathetic innervation of vessels; in contrast it seems to be partly compensated for by an elevated sympathetic activity at least in the UT. As a main cause for the post-immersion o.i. one might suggest a decrease in renin activity.", "contents": "Effect of physical fitness on vanillylmandelic acid excretion during immersion. The effects of 4--6 h head-out immersion on excretion of vanillylmandelic acid (VMA), blood pressure and plasma volume were estimated in 8 endurance-trained (TR) and 8 untrained (UT) subjects. In the trained only a slight increase of VMA excretion occurred (4 h value: +2.7 +/- 10.9 ng/ml GFR), but there was a highly significant increase in the UT (+29.0 +/- 17.2 ng/ml GFR). VMA values during control experiments in supine position tended to decrease in both groups. Systolic and diastolic blood pressure fell by 20 mm Hg after beginning of immersion; in the UT plasma volume was reduced while it remained constant in TR. The results indicate that orthostatic intolerance (o.i.) after immersion is not effected by decreased sympathetic innervation of vessels; in contrast it seems to be partly compensated for by an elevated sympathetic activity at least in the UT. As a main cause for the post-immersion o.i. one might suggest a decrease in renin activity.", "PMID": 976254}
{"id": "pubmed23n0032_10616", "title": "Age-changes of the force-frequency-relationship and the duration of action potential of isolated papillary muscles of guinea pig.", "content": "The force-frequency-relationship and the duration of the action potential recorded intracellularly were compared on isolated isometrically arranged papillary muscles of young (3--4 months old) and senile (36--40 months old) guinea pigs. The muscles were in Krebs-Henseleit-solution at 37 degrees C electrically stimulated with frequencies between 30 and 420 imp./min. The following results were obtained: 1. The isolated papillary muscles of the two groups are almost similar with respect to length, weight and cross-sectional are. 2. In the range of stimulation rate from 30--420 imp./min the duration of action potential in the senile group was 120--135% of that in the young group. 3. The shape of slow repolarization of action potential was steeper in the senile group. 4. The time to peak tension and the relaxation time were longer in the senile group. 5. The maximally developed tension was the same in both groups, but in the senile group it was reached at a stimulation rate of 120 imp./min whereas in the young group, the optimal frequency was 290 imp./min. 6. The greatest value for the maximal rate of tension rise was 2.4 times smaller in the senile group. The results are discussed in relation to the age dependent intensity of membrane currents and to a possible altered activity of sarcoplasmic reticulum.", "contents": "Age-changes of the force-frequency-relationship and the duration of action potential of isolated papillary muscles of guinea pig. The force-frequency-relationship and the duration of the action potential recorded intracellularly were compared on isolated isometrically arranged papillary muscles of young (3--4 months old) and senile (36--40 months old) guinea pigs. The muscles were in Krebs-Henseleit-solution at 37 degrees C electrically stimulated with frequencies between 30 and 420 imp./min. The following results were obtained: 1. The isolated papillary muscles of the two groups are almost similar with respect to length, weight and cross-sectional are. 2. In the range of stimulation rate from 30--420 imp./min the duration of action potential in the senile group was 120--135% of that in the young group. 3. The shape of slow repolarization of action potential was steeper in the senile group. 4. The time to peak tension and the relaxation time were longer in the senile group. 5. The maximally developed tension was the same in both groups, but in the senile group it was reached at a stimulation rate of 120 imp./min whereas in the young group, the optimal frequency was 290 imp./min. 6. The greatest value for the maximal rate of tension rise was 2.4 times smaller in the senile group. The results are discussed in relation to the age dependent intensity of membrane currents and to a possible altered activity of sarcoplasmic reticulum.", "PMID": 976255}
{"id": "pubmed23n0032_10617", "title": "Effect of training on the resting heart rate of rats.", "content": "Adult male rats were progressively trained 5 days/weeks on a motor-driven treadmill. The training period lasted 12 weeks and consisted of 60 min/day of wind-sprints and endurance work. No significant difference in resting heart rates was observed between the control and exercise groups during week 1 (394 +/- 7 vs. 388 +/- 5). However, at week 12 the exercise group had a lower resting heart rate (359 +/- 6 vs. 331 +/- 4). Heart rates observed following saline, propranolol, atropine, and propranolol plus atropine injections were lower in the exercise group in all cases. The difference in heart rates between the control and exercise groups was 19 beats/min following propranolol plus atropine which was less than the 28 beats/min difference observed under control conditions. With atropine and then with propranolol the differences were 33 and 27 beats/min. These heart rate differences were observed without the presence of cardiac hypertrophy as assesssed from ventricle weights. Our data indicate that the bradycardia resulting from exercise training is due primarily to changes other than neural influences on the heart.", "contents": "Effect of training on the resting heart rate of rats. Adult male rats were progressively trained 5 days/weeks on a motor-driven treadmill. The training period lasted 12 weeks and consisted of 60 min/day of wind-sprints and endurance work. No significant difference in resting heart rates was observed between the control and exercise groups during week 1 (394 +/- 7 vs. 388 +/- 5). However, at week 12 the exercise group had a lower resting heart rate (359 +/- 6 vs. 331 +/- 4). Heart rates observed following saline, propranolol, atropine, and propranolol plus atropine injections were lower in the exercise group in all cases. The difference in heart rates between the control and exercise groups was 19 beats/min following propranolol plus atropine which was less than the 28 beats/min difference observed under control conditions. With atropine and then with propranolol the differences were 33 and 27 beats/min. These heart rate differences were observed without the presence of cardiac hypertrophy as assesssed from ventricle weights. Our data indicate that the bradycardia resulting from exercise training is due primarily to changes other than neural influences on the heart.", "PMID": 976256}
{"id": "pubmed23n0032_10618", "title": "Prolonged exercise in prepubertal boys. I. Cardiovascular and metabolic adjustment.", "content": "The circulatory and metabolic changes during prolonged exercise (60 min) were studied in a group of 10 healthy prepubertal boys. The boys worked on the treadmill and on the bicycle ergometer at two levels of load, namely at oxygen uptakes of 36--39%, and 60% of the respective VO2 max for both types of exercise. The identical relative work loads represent higher metabolic rate at the treadmill exercise. The rectal temperature was higher after exercises on treadmill than on bicycle. The heart rate increase during prolonged exercise was lower in boys than in adults in both types of exercise. Better cardiovascular adjustment is suggested. The blood lactate increase was negligible in the initial phase of exercise with subsequent removal from the blood during extended exercise, which was more pronounced at the treadmill exercises. The increasing blood free glycerol concentration indicates similar fat share on energy release in children as in adults. No biological handicap could be found for prolonged exercise in children. The preference of short-time exercises in children lies probably in the psychological sphere.", "contents": "Prolonged exercise in prepubertal boys. I. Cardiovascular and metabolic adjustment. The circulatory and metabolic changes during prolonged exercise (60 min) were studied in a group of 10 healthy prepubertal boys. The boys worked on the treadmill and on the bicycle ergometer at two levels of load, namely at oxygen uptakes of 36--39%, and 60% of the respective VO2 max for both types of exercise. The identical relative work loads represent higher metabolic rate at the treadmill exercise. The rectal temperature was higher after exercises on treadmill than on bicycle. The heart rate increase during prolonged exercise was lower in boys than in adults in both types of exercise. Better cardiovascular adjustment is suggested. The blood lactate increase was negligible in the initial phase of exercise with subsequent removal from the blood during extended exercise, which was more pronounced at the treadmill exercises. The increasing blood free glycerol concentration indicates similar fat share on energy release in children as in adults. No biological handicap could be found for prolonged exercise in children. The preference of short-time exercises in children lies probably in the psychological sphere.", "PMID": 976257}
{"id": "pubmed23n0032_10619", "title": "Prolonged exercise in prepubertal boys. II. Changes in plasma volume and in some blood constituents.", "content": "A group of 10 prepubertal boys was studied during prolonged exercise (60 min) on bicycle ergometer and on treadmill at two levels of work load (appr. 40% and 60% VO2 max). The hematocrit, serum proteins, Cl- and K+ were followed, and from the blood hematocrit changes the plasma volume changes were calculated. At the exercises of lower intensity of both types a slight hemodilution was found (appr. +5% increase in plasma volume), at higher intensity practically no changes could be demonstrated. These findings are supported by the values of serum protein concentration, where no increase was found, and by the fact that at the lower work loads a rather decreasing trend was found for this blood constituent. These findings are at variance with those in adults under similar conditions. The authors suggest that different changes of plasma volume during exercise in boys than in adults could be related to the disparate lactate production and fate in these age groups.", "contents": "Prolonged exercise in prepubertal boys. II. Changes in plasma volume and in some blood constituents. A group of 10 prepubertal boys was studied during prolonged exercise (60 min) on bicycle ergometer and on treadmill at two levels of work load (appr. 40% and 60% VO2 max). The hematocrit, serum proteins, Cl- and K+ were followed, and from the blood hematocrit changes the plasma volume changes were calculated. At the exercises of lower intensity of both types a slight hemodilution was found (appr. +5% increase in plasma volume), at higher intensity practically no changes could be demonstrated. These findings are supported by the values of serum protein concentration, where no increase was found, and by the fact that at the lower work loads a rather decreasing trend was found for this blood constituent. These findings are at variance with those in adults under similar conditions. The authors suggest that different changes of plasma volume during exercise in boys than in adults could be related to the disparate lactate production and fate in these age groups.", "PMID": 976258}
{"id": "pubmed23n0032_10620", "title": "Purification and properties of L-4-hydroxymandelate oxidase from Pseudomonas convexa.", "content": "An inducible membrane-bound L-4-hydroxymandelate oxidase (decarboxylating) from Pseudomonas convexa has been solubilized and partially purified. It catalyzes the conversion of L-4-hydroxymandelic acid to 4-hydroxybenzaldehyde in a single step with the stoichiometric consumption of O2 and liberation of CO2. The enzyme is optimally active at pH 6.6 and at 55 degrees C. It requires FAD and Mn2+ for its activity. The membrane-bound enzyme is more stable than the solubilized and purified enzyme. After solubilization it gradually loses its activity when kept at 5 degrees C which can be fully reactivated by freezing and thawing. The Km values for DL-4-hydroxymandelate and FAD are 0.44 mM and 0.038 mM respectively. The enzyme is highly specific for DL-4-hydroxymandelic acid. DL-3,4-Dihydroxymandelic acid competitively inhibited the enzyme reaction. From the Dixon plot the Ki for DL-3,4-dihydroxymandelic acid was calculated to be 1.8 X 10(-4) M. The enzyme is completely inactivated by thiol compounds and not affected by thiol inhibitors. The enzyme is also inhibited by denaturing agents, heavy metal ions and by chelating agents.", "contents": "Purification and properties of L-4-hydroxymandelate oxidase from Pseudomonas convexa. An inducible membrane-bound L-4-hydroxymandelate oxidase (decarboxylating) from Pseudomonas convexa has been solubilized and partially purified. It catalyzes the conversion of L-4-hydroxymandelic acid to 4-hydroxybenzaldehyde in a single step with the stoichiometric consumption of O2 and liberation of CO2. The enzyme is optimally active at pH 6.6 and at 55 degrees C. It requires FAD and Mn2+ for its activity. The membrane-bound enzyme is more stable than the solubilized and purified enzyme. After solubilization it gradually loses its activity when kept at 5 degrees C which can be fully reactivated by freezing and thawing. The Km values for DL-4-hydroxymandelate and FAD are 0.44 mM and 0.038 mM respectively. The enzyme is highly specific for DL-4-hydroxymandelic acid. DL-3,4-Dihydroxymandelic acid competitively inhibited the enzyme reaction. From the Dixon plot the Ki for DL-3,4-dihydroxymandelic acid was calculated to be 1.8 X 10(-4) M. The enzyme is completely inactivated by thiol compounds and not affected by thiol inhibitors. The enzyme is also inhibited by denaturing agents, heavy metal ions and by chelating agents.", "PMID": 976259}
{"id": "pubmed23n0032_10621", "title": "Gel isoelectric focusing of human-serum transferrin.", "content": "Analysis of human serum transferrin on gel isoelectric focusing resolved this iron-transport protein into two iron-containing components which were identified by the use of radioactively labelled iron and iron-specific stain. These two components were found to be monoferric transferrin (Fe-transferrin) and diferric transferrin (Fe2-transferrin) with isoelectric points of 5.6 and 5.2 respectively. The estimation of the relative proportions of these two components in serum did not correspond to the calculated theoretical ratio based on random binding of iron to the two binding sites of transferrin. However, the analysis of partially resatured apotransferrin gave a ratio corresponding to a random distribution of iron. The significance of these results is discussed.", "contents": "Gel isoelectric focusing of human-serum transferrin. Analysis of human serum transferrin on gel isoelectric focusing resolved this iron-transport protein into two iron-containing components which were identified by the use of radioactively labelled iron and iron-specific stain. These two components were found to be monoferric transferrin (Fe-transferrin) and diferric transferrin (Fe2-transferrin) with isoelectric points of 5.6 and 5.2 respectively. The estimation of the relative proportions of these two components in serum did not correspond to the calculated theoretical ratio based on random binding of iron to the two binding sites of transferrin. However, the analysis of partially resatured apotransferrin gave a ratio corresponding to a random distribution of iron. The significance of these results is discussed.", "PMID": 976260}
{"id": "pubmed23n0032_10622", "title": "Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation.", "content": "In order to elucidate the mechanism of action of inhibitors that block the initiation of protein synthesis in mammalian systems, we have studied the following steps: (a) formation of the ternary complex Met-tRNAr-IF-E2-GTP, (b) binding of the initiator Met-tRNAf to the 40-S ribosomal subunit in the presence of initiation factors and dependent or not on the addition of mRNA, (c) formation of the initiation complex with 80-S ribosomes and (d) formation of the first peptide bond. Adrenochrome, aurintricarboxylic acid, polydextran sulphate, pyrochatechol violet and showdomycin block the formation of the ternary complex Met-tRNAf-IF-E2-GTP. Edeine A1, aurintricarboxylic acid and polydextran sulphate block the binding of the mRNA to the 40-S ribosomal subunit. Pactamycin induces the formation of stable smaller initiation complexes which are unable to go through the subsequent steps of initiation. Stimulation of the binding of the initiator Met-tRNAf to the 80-S ribosome in the presence of initiation factors is observed with sparsomycin and antibiotics of the sesquiterpene family (verrucarin A, trichodermin and trichothecin). However, these antibiotics block the reaction of the bound Met-tRNAf with puromycin. Narciclasine has no effect on the binding of the initiator to the ribosome but strongly blocks its reaction with puromycin. We have developed a simple technique to detect the Met-tRNAf-40-S-subunit-poly(A, G, U) initiation complexes by chromatography on Sepharose 6B columns. The requirements for the formation of such complexes measured by this technique and its comparison with the sucrose gradient centrifugation method are described.", "contents": "Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation. In order to elucidate the mechanism of action of inhibitors that block the initiation of protein synthesis in mammalian systems, we have studied the following steps: (a) formation of the ternary complex Met-tRNAr-IF-E2-GTP, (b) binding of the initiator Met-tRNAf to the 40-S ribosomal subunit in the presence of initiation factors and dependent or not on the addition of mRNA, (c) formation of the initiation complex with 80-S ribosomes and (d) formation of the first peptide bond. Adrenochrome, aurintricarboxylic acid, polydextran sulphate, pyrochatechol violet and showdomycin block the formation of the ternary complex Met-tRNAf-IF-E2-GTP. Edeine A1, aurintricarboxylic acid and polydextran sulphate block the binding of the mRNA to the 40-S ribosomal subunit. Pactamycin induces the formation of stable smaller initiation complexes which are unable to go through the subsequent steps of initiation. Stimulation of the binding of the initiator Met-tRNAf to the 80-S ribosome in the presence of initiation factors is observed with sparsomycin and antibiotics of the sesquiterpene family (verrucarin A, trichodermin and trichothecin). However, these antibiotics block the reaction of the bound Met-tRNAf with puromycin. Narciclasine has no effect on the binding of the initiator to the ribosome but strongly blocks its reaction with puromycin. We have developed a simple technique to detect the Met-tRNAf-40-S-subunit-poly(A, G, U) initiation complexes by chromatography on Sepharose 6B columns. The requirements for the formation of such complexes measured by this technique and its comparison with the sucrose gradient centrifugation method are described.", "PMID": 976261}
{"id": "pubmed23n0032_10623", "title": "Isolation and characterization of the lipopolysaccharide of Chromatium vinosum.", "content": "Lipolysaccharide was isolated from Chromatium vinosum by phenol/water extraction. The lipopolysaccharide is found exclusively in the phenol phase and can be cleaved into a sugar moiety and a lipid A fraction by hydrolysis in 10% acetic acid at 100 degrees C for 3-4 h. The sugar moiety contains the neutral sugars 3-O-methyl-D-ribose, D-ribose, L-arabinose, mannosamine and glucose, and smaller quantities of D-rhamnose, D-glycero-D-manno-heptose (tentatively identified), quinovosamine and 2-keto-3-deoxyoctonate. L-glycero-D-manno-heptose was not detected. The 2-keto-3-deoxyoctonate linkage in C. vinosum lipopolysaccharide is more resistant to acid hydrolysis than that of Escherichia coli. The lipid A fraction contains glucosamine, mannose and the fatty acids of the lipopolysaccharide. The major fatty acid is beta-hydroxymyristic acid, with smaller amounts of lauric and palmitic acids as well as 14-carbon mono-unsaturated fatty acid, also being present. The phosphorus content of the C. vinosum lipopolysaccharide was found to be approximately 0.1%. Erythrocytes sensitized with alkali-treated C. vinosum lipopolysaccharide were agglutinated by antisera prepared against heat-killed cells. Untreated or heat-treated lipopolysaccharide did not sensitize erythrocytes. The lethal toxicity to mice of the C. vinosum lipopolysaccharide is about one-tenth as that from Salmonella abortus equi.", "contents": "Isolation and characterization of the lipopolysaccharide of Chromatium vinosum. Lipolysaccharide was isolated from Chromatium vinosum by phenol/water extraction. The lipopolysaccharide is found exclusively in the phenol phase and can be cleaved into a sugar moiety and a lipid A fraction by hydrolysis in 10% acetic acid at 100 degrees C for 3-4 h. The sugar moiety contains the neutral sugars 3-O-methyl-D-ribose, D-ribose, L-arabinose, mannosamine and glucose, and smaller quantities of D-rhamnose, D-glycero-D-manno-heptose (tentatively identified), quinovosamine and 2-keto-3-deoxyoctonate. L-glycero-D-manno-heptose was not detected. The 2-keto-3-deoxyoctonate linkage in C. vinosum lipopolysaccharide is more resistant to acid hydrolysis than that of Escherichia coli. The lipid A fraction contains glucosamine, mannose and the fatty acids of the lipopolysaccharide. The major fatty acid is beta-hydroxymyristic acid, with smaller amounts of lauric and palmitic acids as well as 14-carbon mono-unsaturated fatty acid, also being present. The phosphorus content of the C. vinosum lipopolysaccharide was found to be approximately 0.1%. Erythrocytes sensitized with alkali-treated C. vinosum lipopolysaccharide were agglutinated by antisera prepared against heat-killed cells. Untreated or heat-treated lipopolysaccharide did not sensitize erythrocytes. The lethal toxicity to mice of the C. vinosum lipopolysaccharide is about one-tenth as that from Salmonella abortus equi.", "PMID": 976262}
{"id": "pubmed23n0032_10624", "title": "Affinity labelling of the estradiol-17 beta dehydrogenase from human placenta with substrate analogs.", "content": "Affinity labelling of the estradiol-17 beta dehydrogenase of human placenta has been performed using derivatives of estradiol-17 beta carrying alkylating groups in nine different positions on the steroid nucleus. The active-site-directed character of the inhibition is confirmed by the following observations: the affinity labels are substrates or competitive inhibitors, the enzyme is protected against inactivation and alkylation by the substrate and by the coenzyme, the stoichiometry of the alkylation is two moles of inhibitor per 68 000 g of enzyme (dimer). The alkylation of a histidine residue which is fast and extensive when the alkylation side chain is on the C-3 carbon atom, is dramatically decreased when alkylating side chain is shifted towards rings B and D. These results allow the location of this histidine in the vicinity of ring A and probably on the beta face of the steroid nucleus. The reactivity of a cysteine located on the active site was quite different, showing increasing alkylation when the alkylating substituent of the affinity labels was shifted from C-3 to C-16 of the steroid nucleus. The correlation of this result and that obtained using an alkylating analog of NAD (3-chloroacetyl-pyridine-adenine dinucleotide) indicates that this cysteine is located in the catalytic region of the active site, at the junction of the ring D of the steroid nucleus with the nicotinamide moiety of the coenzyme.", "contents": "Affinity labelling of the estradiol-17 beta dehydrogenase from human placenta with substrate analogs. Affinity labelling of the estradiol-17 beta dehydrogenase of human placenta has been performed using derivatives of estradiol-17 beta carrying alkylating groups in nine different positions on the steroid nucleus. The active-site-directed character of the inhibition is confirmed by the following observations: the affinity labels are substrates or competitive inhibitors, the enzyme is protected against inactivation and alkylation by the substrate and by the coenzyme, the stoichiometry of the alkylation is two moles of inhibitor per 68 000 g of enzyme (dimer). The alkylation of a histidine residue which is fast and extensive when the alkylation side chain is on the C-3 carbon atom, is dramatically decreased when alkylating side chain is shifted towards rings B and D. These results allow the location of this histidine in the vicinity of ring A and probably on the beta face of the steroid nucleus. The reactivity of a cysteine located on the active site was quite different, showing increasing alkylation when the alkylating substituent of the affinity labels was shifted from C-3 to C-16 of the steroid nucleus. The correlation of this result and that obtained using an alkylating analog of NAD (3-chloroacetyl-pyridine-adenine dinucleotide) indicates that this cysteine is located in the catalytic region of the active site, at the junction of the ring D of the steroid nucleus with the nicotinamide moiety of the coenzyme.", "PMID": 976263}
{"id": "pubmed23n0032_10625", "title": "Role of GTP in eukaryotic polypeptide-chain initiation. Purification and properties of a factor from Artemia salina embryos which interacts with initiator transfer RNA and guanine nucleotides.", "content": "1. A factor, which makes a ternary complex with GTP and eukaryotic initiator tRNA (Met-tRNAi), has been purified 100-fold from developed cysts of Artemia salina. Some of the properties of the purified factor have been studied. 2. Mg2+ appears to inhibit ternary complex formation. 3. Little or no ternary complex is formed when 5 muM GTP is replaced by an identical concentration of UTP, CTP or ATP. The analog, guanosine 5'-(beta, gamma-imino)triphosphate [GMP-P(NHP)] seems to be a much better substitute for GTP than guanosine 5'-(beta, gamma-methylene)triphosphate [GMP-P(CH2)P]. Since GMP-P(NH)P is as effective as GTP in ternary complex formation, it would appear that GTP plays the role of an allosteric effector in this step of eukaryotic polypeptide chain initiation. 4. GDP inhibits both the rate and extent of ternary complex formation. The inhibition is largely reversed by adding a 5-fold molar excess of GTP over GDP. DGDP is slightly less inhibitory than GDP. UDP and CDP are much less inhibitory than GDP and very little inhibition is obtained with ADP. 5. The preformed ternary complex is rapidly and completely destroyed in the presence of N-ethylmaleimide. The results suggest that free--SH groups of the factor may be essential for maintaining the integrity of the ternary complex.", "contents": "Role of GTP in eukaryotic polypeptide-chain initiation. Purification and properties of a factor from Artemia salina embryos which interacts with initiator transfer RNA and guanine nucleotides. 1. A factor, which makes a ternary complex with GTP and eukaryotic initiator tRNA (Met-tRNAi), has been purified 100-fold from developed cysts of Artemia salina. Some of the properties of the purified factor have been studied. 2. Mg2+ appears to inhibit ternary complex formation. 3. Little or no ternary complex is formed when 5 muM GTP is replaced by an identical concentration of UTP, CTP or ATP. The analog, guanosine 5'-(beta, gamma-imino)triphosphate [GMP-P(NHP)] seems to be a much better substitute for GTP than guanosine 5'-(beta, gamma-methylene)triphosphate [GMP-P(CH2)P]. Since GMP-P(NH)P is as effective as GTP in ternary complex formation, it would appear that GTP plays the role of an allosteric effector in this step of eukaryotic polypeptide chain initiation. 4. GDP inhibits both the rate and extent of ternary complex formation. The inhibition is largely reversed by adding a 5-fold molar excess of GTP over GDP. DGDP is slightly less inhibitory than GDP. UDP and CDP are much less inhibitory than GDP and very little inhibition is obtained with ADP. 5. The preformed ternary complex is rapidly and completely destroyed in the presence of N-ethylmaleimide. The results suggest that free--SH groups of the factor may be essential for maintaining the integrity of the ternary complex.", "PMID": 976264}
{"id": "pubmed23n0032_10626", "title": "Effects of thyroid hormone deficiency on the distribution of hepatic metabolites and control of pathways of carbohydrate metabolism in liver and adipose tissue of the rat.", "content": "1. Measurements have been made of the hepatic metabolites in normal and thyroidectomized rats and enzymes of the glycolytic route, the pentose phosphate pathway, the tricarboxylic acid cycle and lipogenesis together with the flux of glucose through alternative pathways of glucose metabolism and into lipid. 2. There is a significant fall in the content of ADP, AMP, citrate, long-chain acyl-CoA derivatives and a rise of 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate following thyroidectomy. The observed changes in the glycolytic intermediates may be correlated with the increased activity of phosphofructokinase relative to pyruvate kinase and fructose bisphosphatase. 3. The NAD+/NADH quotient of the mitochondrial compartment, calculated from the reactants and Keq of 3-hydroxybutyrate dehydrogenase, becomes significantly more oxidized in hypothyroid animals. The redox state of the cytosolic NAD and NADP couples remains relatively unchanged. 4. The changes in the hepatic content of CoA derivatives and citrate and in the mitochondrial redox state are interpreted as indicating a depressed rate of lipid oxidation. 5. Calculations of the compartmentation of metabolites between the cytosol and mitochondria indicate a very marked decrease in mitochondrial citrate, 2-oxoglutarate and glutamate with smaller changes in aspartate and malate. These changes are interpreted as providing evidence for the importance of modifications in the malate-aspartate shuttle in hypothyroidism; this is further supported by measured changes in the distribution and activities of the component enzymes of the hydrogen shuttles. 6. There is a diminished activity of glucokinase and of enzymes of the glycolytic pathway below phosphofructokinase in livers from hypothyroid rats. The oxidative enzymes of the pentose phosphate pathway, ATP-citrate lyase, 'malic' enzyme and fatty acid synthetase also decrease markedly. There is a striking parallelism between the changes of enzyme profile of liver and adipose tissue in hypothyroidism. 7. Adrenal glands from hypothyroid rats showed a generalized delcine in enzyme activity in parallel with the fall in tissue weight. Cytosolic glycerol-3-phosphate dehydrogenase decreased sharply. 8. The present results are discussed in relation to data in the literature on the increased activities of cyclic AMP and cyclic GMP phosphodiesterases in hypothyroidism, observations which integrate the relationships between anabolic and catabolic pathways of carbohydrate and lipid metabolism with alterations in hormone sensitivity in hypothyroidism.", "contents": "Effects of thyroid hormone deficiency on the distribution of hepatic metabolites and control of pathways of carbohydrate metabolism in liver and adipose tissue of the rat. 1. Measurements have been made of the hepatic metabolites in normal and thyroidectomized rats and enzymes of the glycolytic route, the pentose phosphate pathway, the tricarboxylic acid cycle and lipogenesis together with the flux of glucose through alternative pathways of glucose metabolism and into lipid. 2. There is a significant fall in the content of ADP, AMP, citrate, long-chain acyl-CoA derivatives and a rise of 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate following thyroidectomy. The observed changes in the glycolytic intermediates may be correlated with the increased activity of phosphofructokinase relative to pyruvate kinase and fructose bisphosphatase. 3. The NAD+/NADH quotient of the mitochondrial compartment, calculated from the reactants and Keq of 3-hydroxybutyrate dehydrogenase, becomes significantly more oxidized in hypothyroid animals. The redox state of the cytosolic NAD and NADP couples remains relatively unchanged. 4. The changes in the hepatic content of CoA derivatives and citrate and in the mitochondrial redox state are interpreted as indicating a depressed rate of lipid oxidation. 5. Calculations of the compartmentation of metabolites between the cytosol and mitochondria indicate a very marked decrease in mitochondrial citrate, 2-oxoglutarate and glutamate with smaller changes in aspartate and malate. These changes are interpreted as providing evidence for the importance of modifications in the malate-aspartate shuttle in hypothyroidism; this is further supported by measured changes in the distribution and activities of the component enzymes of the hydrogen shuttles. 6. There is a diminished activity of glucokinase and of enzymes of the glycolytic pathway below phosphofructokinase in livers from hypothyroid rats. The oxidative enzymes of the pentose phosphate pathway, ATP-citrate lyase, 'malic' enzyme and fatty acid synthetase also decrease markedly. There is a striking parallelism between the changes of enzyme profile of liver and adipose tissue in hypothyroidism. 7. Adrenal glands from hypothyroid rats showed a generalized delcine in enzyme activity in parallel with the fall in tissue weight. Cytosolic glycerol-3-phosphate dehydrogenase decreased sharply. 8. The present results are discussed in relation to data in the literature on the increased activities of cyclic AMP and cyclic GMP phosphodiesterases in hypothyroidism, observations which integrate the relationships between anabolic and catabolic pathways of carbohydrate and lipid metabolism with alterations in hormone sensitivity in hypothyroidism.", "PMID": 976265}
{"id": "pubmed23n0032_10627", "title": "Globin messenger RNA from anaemic rabbit spleen. Size of its polyadenylate segment.", "content": "The size of the polyadenylate segment of globin messenger RNA isolated from spleens of anaemic rabbits was estimated by comparison of its electrophoretic migration in polyacrylamide gels to that of synthetic poly(A) segments of known lengths. Conditions of enzymic degradation of mRNA with pancreatic ribonuclease and T1 ribonuclease were carefully established in order to ensure complete degradation of the heteropolymeric part of mRNA without affecting the polyadenylate sequence. The poly (A) segments of spleen globin mRNA were found to be 25-90 nucleotides long whilst those of peripheral blood reticulocytes from the same animals were only 10-30 residues long. Since spleen contains young erythroid cells and since anucleated blood reticulocytes constitute a statistically older population of the same cell line, these results support the idea that the poly(A) segment of mRNA shortens when the message ages.", "contents": "Globin messenger RNA from anaemic rabbit spleen. Size of its polyadenylate segment. The size of the polyadenylate segment of globin messenger RNA isolated from spleens of anaemic rabbits was estimated by comparison of its electrophoretic migration in polyacrylamide gels to that of synthetic poly(A) segments of known lengths. Conditions of enzymic degradation of mRNA with pancreatic ribonuclease and T1 ribonuclease were carefully established in order to ensure complete degradation of the heteropolymeric part of mRNA without affecting the polyadenylate sequence. The poly (A) segments of spleen globin mRNA were found to be 25-90 nucleotides long whilst those of peripheral blood reticulocytes from the same animals were only 10-30 residues long. Since spleen contains young erythroid cells and since anucleated blood reticulocytes constitute a statistically older population of the same cell line, these results support the idea that the poly(A) segment of mRNA shortens when the message ages.", "PMID": 976266}
{"id": "pubmed23n0032_10628", "title": "Purification of six neurotoxins from the venom of Dendroaspis viridis. Primary structure of two long toxins.", "content": "Six neurotoxins were purified from Dendroaspis viridis venom using gel filtration and equilibrium chromatography. The amino acid sequences of two of these neurotoxins (72 and 73 residues, five disulphide bridges) have been determined using almost exclusively automated Edman degradation. These two sequences are very similar: the only differences lies in the presence of one extra glycine at the C-terminal end of one of them. There is a good homology with the sequences of toxins now isolated from other Elapidae and Hydrophidae venoms.", "contents": "Purification of six neurotoxins from the venom of Dendroaspis viridis. Primary structure of two long toxins. Six neurotoxins were purified from Dendroaspis viridis venom using gel filtration and equilibrium chromatography. The amino acid sequences of two of these neurotoxins (72 and 73 residues, five disulphide bridges) have been determined using almost exclusively automated Edman degradation. These two sequences are very similar: the only differences lies in the presence of one extra glycine at the C-terminal end of one of them. There is a good homology with the sequences of toxins now isolated from other Elapidae and Hydrophidae venoms.", "PMID": 976267}
{"id": "pubmed23n0032_10629", "title": "Taipoxin, an extremely potent presynaptic neurotoxin from the venom of the australian snake taipan (Oxyuranus s. scutellatus). Isolation, characterization, quaternary structure and pharmacological properties.", "content": "Taipoxin (taipan toxin), purified from the venom of the Australian taipan (Oxyuranus s. scutellatus) by gel filtration on Sephadex G-75 followed by column zone electrophoresis, is the most lethal neurotoxin yet isolated from any snake venom. The LD50 is 2 mug/kg in the mouse. The main physiological effect is a gradual reduction to complete stop of evoked and spontaneous release of acetylcholine from motor nerve terminals. Intoxicated animals die of asphyxia caused by neuromuscular blockage of the respiratory muscles. Taipoxin is a moderately acidic sialo-glycoprotein (pI 5) with a molecular weight of 45 600 as calculated from composition data or 46 800 as determined by meniscus depletion sedimentation equilibrium. Taipoxin is a 1:1:1 ternary complex of subunits designated alpha, beta and gamma which dissociate completely at low pH and high ionic strength or in 6 M guanidine hydrochloride. The dissociation by guanidine at neutral pH is reversible, while the acid-induced dissociation is not . The alpha and beta components consist of 120 amino acid residues cross-linked by seven disulfide bridges, whereas the gamma component has 135 residues and eight disulfides. The very basic (pI Greater than 10) alpha component contains 13 residues of arginine and is the only subunit displaying lethal neurotoxicity (mouse LD50 approximately 300 mug/kg). The neutral beta fraction was separated by ion-exchange chromatography into two iso-component, beta 1 and beta2, which differ slightly in amino acid composition. The very acidic gamma component contains all of the carbohydrate, which includes 4-5 residues of sialic aid. The three subunits are homologous in sequence although the gamma component is eight residues longer on the N-terminus and must also contain extra amino acids elsewhere.", "contents": "Taipoxin, an extremely potent presynaptic neurotoxin from the venom of the australian snake taipan (Oxyuranus s. scutellatus). Isolation, characterization, quaternary structure and pharmacological properties. Taipoxin (taipan toxin), purified from the venom of the Australian taipan (Oxyuranus s. scutellatus) by gel filtration on Sephadex G-75 followed by column zone electrophoresis, is the most lethal neurotoxin yet isolated from any snake venom. The LD50 is 2 mug/kg in the mouse. The main physiological effect is a gradual reduction to complete stop of evoked and spontaneous release of acetylcholine from motor nerve terminals. Intoxicated animals die of asphyxia caused by neuromuscular blockage of the respiratory muscles. Taipoxin is a moderately acidic sialo-glycoprotein (pI 5) with a molecular weight of 45 600 as calculated from composition data or 46 800 as determined by meniscus depletion sedimentation equilibrium. Taipoxin is a 1:1:1 ternary complex of subunits designated alpha, beta and gamma which dissociate completely at low pH and high ionic strength or in 6 M guanidine hydrochloride. The dissociation by guanidine at neutral pH is reversible, while the acid-induced dissociation is not . The alpha and beta components consist of 120 amino acid residues cross-linked by seven disulfide bridges, whereas the gamma component has 135 residues and eight disulfides. The very basic (pI Greater than 10) alpha component contains 13 residues of arginine and is the only subunit displaying lethal neurotoxicity (mouse LD50 approximately 300 mug/kg). The neutral beta fraction was separated by ion-exchange chromatography into two iso-component, beta 1 and beta2, which differ slightly in amino acid composition. The very acidic gamma component contains all of the carbohydrate, which includes 4-5 residues of sialic aid. The three subunits are homologous in sequence although the gamma component is eight residues longer on the N-terminus and must also contain extra amino acids elsewhere.", "PMID": 976268}
{"id": "pubmed23n0032_10630", "title": "The arrangement of 18-S and 28-S ribosomal ribonucleic acids within the 40-S precursor molecule of Xenopus laevis.", "content": "The arrangement of 18-S rRNA and 28-S rRNA within their 40-S common precursor molecule (pre-rRNA) of Xenopus laevis was investigated by electron microscopic analysis of secondary structure of nascent pre-rRNA chains of oocytes, and by 5'-end analysis of 18-S rRNA and 28-S rRNA hybridized to the EcoRI fragment of rDNA cloned as plasmid pCD42. Secondary structure mapping of phenol-extracted RNA from nucleolar cores revealed complete pre-rRNA chains or molecules at various stages of processing and pre-rRNA molecules apparently lacking one end. In this latter group, which was regarded as representing nascent chains, more than 90% of the molecules had no 28-S rRNA REGION. This shows that the 28-S rRNA sequence is transcribed after the 18-S rRNA region and hence must be located nearer to the 3' end of the pre-rRNA molecule. For 5' end-group determination [3H]uridine-labelled 18-S rRNA and 28-S rRNA were hybridized, as fragments of about 200 nucleotides, to the plasmid pCD42 containing coding sequences for four-fifths of the 18-S rRNA sequence, the external transcribed spacer, the non-transcribed spacer and a tenth of the 28-S rRNA sequence. The RNA was recovered from the hybrids and analyzed for uridine 3',5'-bisphosphate (pUp) after alkaline hydrolysis. The pUp content of the hybridized 18-S rRNA fragments was 20-fold higher than in those of 28-S rRNA, THUS DEMONSTRATING THAT THE 5' END OF THE 18-S rRNA is located next to the external spacer region. From these results it is concluded that the 18-S rRNA is located close to the 5' end of the 40-S pre-rRNA molecule.", "contents": "The arrangement of 18-S and 28-S ribosomal ribonucleic acids within the 40-S precursor molecule of Xenopus laevis. The arrangement of 18-S rRNA and 28-S rRNA within their 40-S common precursor molecule (pre-rRNA) of Xenopus laevis was investigated by electron microscopic analysis of secondary structure of nascent pre-rRNA chains of oocytes, and by 5'-end analysis of 18-S rRNA and 28-S rRNA hybridized to the EcoRI fragment of rDNA cloned as plasmid pCD42. Secondary structure mapping of phenol-extracted RNA from nucleolar cores revealed complete pre-rRNA chains or molecules at various stages of processing and pre-rRNA molecules apparently lacking one end. In this latter group, which was regarded as representing nascent chains, more than 90% of the molecules had no 28-S rRNA REGION. This shows that the 28-S rRNA sequence is transcribed after the 18-S rRNA region and hence must be located nearer to the 3' end of the pre-rRNA molecule. For 5' end-group determination [3H]uridine-labelled 18-S rRNA and 28-S rRNA were hybridized, as fragments of about 200 nucleotides, to the plasmid pCD42 containing coding sequences for four-fifths of the 18-S rRNA sequence, the external transcribed spacer, the non-transcribed spacer and a tenth of the 28-S rRNA sequence. The RNA was recovered from the hybrids and analyzed for uridine 3',5'-bisphosphate (pUp) after alkaline hydrolysis. The pUp content of the hybridized 18-S rRNA fragments was 20-fold higher than in those of 28-S rRNA, THUS DEMONSTRATING THAT THE 5' END OF THE 18-S rRNA is located next to the external spacer region. From these results it is concluded that the 18-S rRNA is located close to the 5' end of the 40-S pre-rRNA molecule.", "PMID": 976269}
{"id": "pubmed23n0032_10631", "title": "Acceleration of the reaction between thrombin and antithrombin III by non-stoichiometric amounts of heparin.", "content": "The accelerating effect of heparin on the reaction between purified human antithrombin and thrombin has been investigated by measuring the amount of thrombin inactivated during a short incubation of the enzyme with the inhibitor in the presence and absence of non-stoichiometric amounts of heparin. It was demonstrated that one molecule of heparin was able to promote the binding of a large number of antithrombin molecules to thrombin. Thus heparin may affect the rate of the inactivation of thrombin by antithrombin in a catalytic manner.", "contents": "Acceleration of the reaction between thrombin and antithrombin III by non-stoichiometric amounts of heparin. The accelerating effect of heparin on the reaction between purified human antithrombin and thrombin has been investigated by measuring the amount of thrombin inactivated during a short incubation of the enzyme with the inhibitor in the presence and absence of non-stoichiometric amounts of heparin. It was demonstrated that one molecule of heparin was able to promote the binding of a large number of antithrombin molecules to thrombin. Thus heparin may affect the rate of the inactivation of thrombin by antithrombin in a catalytic manner.", "PMID": 976270}
{"id": "pubmed23n0032_10632", "title": "Molecular forms of Electrophorus acetylcholinesterase. Molecular weight and composition.", "content": "Molecular weights for the series of six Electrophorus acetylcholinesterase forms have been determined either by the sedimentation-diffusion equilibrium method or, particularly in the case of the very scarce G' and G inches forms, from their Stokes radius and sedimentation coefficient values. Both methods are in excellent agreement. The results provide good evidence for the model previously proposed, G inches, G' and G containing one, two and four subunits, whereas A, C and D possess, in addition to respectively one, two and three tetrameric sets of such subunits, a structural element, the tail. Although the amino acid composition of 'tailed' and globular forms did not reveal any significant feature of this element, its mass, about 100 000 daltons, could be deduced from a comparison of molecular weights for the two classes of acetylcholinesterase forms. This value is in close agreement with electron microscopic data. The tail is thought to consist of three 30 000-dalton strands.", "contents": "Molecular forms of Electrophorus acetylcholinesterase. Molecular weight and composition. Molecular weights for the series of six Electrophorus acetylcholinesterase forms have been determined either by the sedimentation-diffusion equilibrium method or, particularly in the case of the very scarce G' and G inches forms, from their Stokes radius and sedimentation coefficient values. Both methods are in excellent agreement. The results provide good evidence for the model previously proposed, G inches, G' and G containing one, two and four subunits, whereas A, C and D possess, in addition to respectively one, two and three tetrameric sets of such subunits, a structural element, the tail. Although the amino acid composition of 'tailed' and globular forms did not reveal any significant feature of this element, its mass, about 100 000 daltons, could be deduced from a comparison of molecular weights for the two classes of acetylcholinesterase forms. This value is in close agreement with electron microscopic data. The tail is thought to consist of three 30 000-dalton strands.", "PMID": 976271}
{"id": "pubmed23n0032_10633", "title": "Affinity chromatography of acetylcholinesterase. The importance of hydrophobic interactions.", "content": "An easily prepared affinity column for acetylcholinesterase is described, which may be operated at ionic strength high enough to prevent aggregation of the asymmetric forms of the enzyme. Specific elution by tetraethylammonium or decamethonium was quantitative. The performance of this column is comparable to that of the column described by Dudai and Silman. It is shown that the hexyl 'spacer arm' strongly participates in the enzyme binding and that its replacement with the more hydrophilic spermine chain lowers the affinity. The hexyl chain itself is shown to bind acetylcholinesterase, although with lower affinity and capacity than the complete column. This binding is also partly reversed by inhibitors. Such hydrophobic columns bind the native asymmetric forms of the enzyme more strongly than the lytic globular ones. The aromatic quaternary ligang inhibits Electrophorus but not Torpedo acetylcholinesterase; therefore the column does not retain the Torpedo enzyme. Differences in Km between acetylcholinesterases of the two species also point to differences in their active sites.", "contents": "Affinity chromatography of acetylcholinesterase. The importance of hydrophobic interactions. An easily prepared affinity column for acetylcholinesterase is described, which may be operated at ionic strength high enough to prevent aggregation of the asymmetric forms of the enzyme. Specific elution by tetraethylammonium or decamethonium was quantitative. The performance of this column is comparable to that of the column described by Dudai and Silman. It is shown that the hexyl 'spacer arm' strongly participates in the enzyme binding and that its replacement with the more hydrophilic spermine chain lowers the affinity. The hexyl chain itself is shown to bind acetylcholinesterase, although with lower affinity and capacity than the complete column. This binding is also partly reversed by inhibitors. Such hydrophobic columns bind the native asymmetric forms of the enzyme more strongly than the lytic globular ones. The aromatic quaternary ligang inhibits Electrophorus but not Torpedo acetylcholinesterase; therefore the column does not retain the Torpedo enzyme. Differences in Km between acetylcholinesterases of the two species also point to differences in their active sites.", "PMID": 976272}
{"id": "pubmed23n0032_10634", "title": "Chemical modification of the tryptophan residues of wheat-germ agglutinin. Effect on fluorescence and saccharide-binding properties.", "content": "The oxidation of the tryptophan residues of wheat germ agglutinin by N-bromosuccinimide was investigated under non-denaturing and denaturing conditions. All three tryptophan residues present in wheat germ agglutinin subunit (molecular weight 18 000) could be modified in 0.1 M acetic acid/8 M urea, pH 3.9. One of the residues failed, however, to react with N-bromosuccinimide when the modification was in 0.1 M citrate buffer, pH 6.0. Tryptophan fluorescence of the protein was quenched concomitantly with the oxidation of two tryptophan residues even when the modification was carried out in acetic acid urea. After oxidation of two tryptophan residues per subunit of wheat germ agglutinin, only 15% of the original tryptophan fluorescence remained; upon excitation at 280 nm, tyrosine fluorescence centered at 305 nm could be resolved. The results suggest that there are only two emitters in the protein and that the third tryptophan residue is buried in the native protein and can be modified only in acetic acid urea. This tryptophan residue is quenched in the native protein. Saturation of wheat germ agglutinin with tri-N-acetylchitotriose did not protect the tryptophan residues from oxidation by N-bromosuccinimide. Under these conditions, however, the reactivity of the tryptophan residues towards N-bromosuccinimide was reduced and a higher concentration of the reagent was required to achieve the same extent of oxidation as in the absence of the saccharide. Oxidation of one tryptophan residue per subunit in acetic acid urea led to almost complete loss (97%) of hemagglutinating activity, a 3.5-fold decrease in the affinity constant for tri-N-acetylchitotriose and loss of ability of the subunits (SO20,w = 2.0 S) to reassociate to the native dimer (So20,w = 3.5 S) after dialysis against a non-denaturing buffer. No significant changes in the circular dichroism spectrum of wheat germ agglutinin were observed after oxidation of the three tryptophan residues, suggesting that no gross conformational changes occurred. The steric relationships between the fluorescent tryptophan residues of wheat germ agglutinin and saccharides are discussed.", "contents": "Chemical modification of the tryptophan residues of wheat-germ agglutinin. Effect on fluorescence and saccharide-binding properties. The oxidation of the tryptophan residues of wheat germ agglutinin by N-bromosuccinimide was investigated under non-denaturing and denaturing conditions. All three tryptophan residues present in wheat germ agglutinin subunit (molecular weight 18 000) could be modified in 0.1 M acetic acid/8 M urea, pH 3.9. One of the residues failed, however, to react with N-bromosuccinimide when the modification was in 0.1 M citrate buffer, pH 6.0. Tryptophan fluorescence of the protein was quenched concomitantly with the oxidation of two tryptophan residues even when the modification was carried out in acetic acid urea. After oxidation of two tryptophan residues per subunit of wheat germ agglutinin, only 15% of the original tryptophan fluorescence remained; upon excitation at 280 nm, tyrosine fluorescence centered at 305 nm could be resolved. The results suggest that there are only two emitters in the protein and that the third tryptophan residue is buried in the native protein and can be modified only in acetic acid urea. This tryptophan residue is quenched in the native protein. Saturation of wheat germ agglutinin with tri-N-acetylchitotriose did not protect the tryptophan residues from oxidation by N-bromosuccinimide. Under these conditions, however, the reactivity of the tryptophan residues towards N-bromosuccinimide was reduced and a higher concentration of the reagent was required to achieve the same extent of oxidation as in the absence of the saccharide. Oxidation of one tryptophan residue per subunit in acetic acid urea led to almost complete loss (97%) of hemagglutinating activity, a 3.5-fold decrease in the affinity constant for tri-N-acetylchitotriose and loss of ability of the subunits (SO20,w = 2.0 S) to reassociate to the native dimer (So20,w = 3.5 S) after dialysis against a non-denaturing buffer. No significant changes in the circular dichroism spectrum of wheat germ agglutinin were observed after oxidation of the three tryptophan residues, suggesting that no gross conformational changes occurred. The steric relationships between the fluorescent tryptophan residues of wheat germ agglutinin and saccharides are discussed.", "PMID": 976273}
{"id": "pubmed23n0032_10635", "title": "Nanosecond-pulse fluorimetry of wheat-germ agglutinin (lectin).", "content": "Nanosecond-pulse fluorimetry of wheat germ agglutinin is analyzed as a function of both excitation and emission wavelengths. When excited at 280 nm, wheat germ agglutinin fluorescence exhibited three lifetimes: one corresponding to the tyrosine residues as a whole and two others corresponding to the tryptophyl emission. The tyrosine contribution to the emission spectrum deduced from this method was in good agreement with that reported previously in steady-state fluorescence experiments [Privat, J.P. and Monsigny, M. (1975) Eur. J. Biochem. 60, 555-567]. The fluorescence decay of each tryptophan residue was not a single exponential function when wheat germ agglutinin was excited at 295 nm. This could be related to the microenvironment of the indole chromophores in the protein. The comparison of the quantum yield and of average lifetime showed that some tryptophan residues were completely quenched. Energy transfer from tyrosines to tryptophan residues previously detected in steady-state fluorescence was also revealed by fluorescence decay measurements. Comparison of both methods showed that an important part of transfers occurred with a very fast rate equal to or greater than 10(10) s-1. Both lifetimes and the ratio of the short and the long-lived component were found dependent on tri-N-acetylchitotriose binding.", "contents": "Nanosecond-pulse fluorimetry of wheat-germ agglutinin (lectin). Nanosecond-pulse fluorimetry of wheat germ agglutinin is analyzed as a function of both excitation and emission wavelengths. When excited at 280 nm, wheat germ agglutinin fluorescence exhibited three lifetimes: one corresponding to the tyrosine residues as a whole and two others corresponding to the tryptophyl emission. The tyrosine contribution to the emission spectrum deduced from this method was in good agreement with that reported previously in steady-state fluorescence experiments [Privat, J.P. and Monsigny, M. (1975) Eur. J. Biochem. 60, 555-567]. The fluorescence decay of each tryptophan residue was not a single exponential function when wheat germ agglutinin was excited at 295 nm. This could be related to the microenvironment of the indole chromophores in the protein. The comparison of the quantum yield and of average lifetime showed that some tryptophan residues were completely quenched. Energy transfer from tyrosines to tryptophan residues previously detected in steady-state fluorescence was also revealed by fluorescence decay measurements. Comparison of both methods showed that an important part of transfers occurred with a very fast rate equal to or greater than 10(10) s-1. Both lifetimes and the ratio of the short and the long-lived component were found dependent on tri-N-acetylchitotriose binding.", "PMID": 976274}
{"id": "pubmed23n0032_10636", "title": "6-Methylsalicylic acid synthetase from Penicillium patulum. Some catalytic properties of the enzyme and its relation to fatty acid synthetase.", "content": "1. The specificity of 6-methylsalicylic acid synthetase with respect to the priming substrate was studied. If acetyl-CoA was replaced by propionyl-CoA 6-ethylsalicylic acid was synthesized. The rate of this synthesis was about 13% that of 6-methylsalicylic acid synthesis in presence of acetyl-CoA. 2. 6-Methylsalicylic acid synthetase contains an acetyl transferase activity as demonstrated by the transfer of acetyl residues from acetyl-CoA to pantetheine. This transferase also catalyses propionyl transfer but only with an about 13 times slower rate. 3. Analogous to fatty acid synthetase, treatment with iodoacetamide converts 6-methylsalicylic acid synthetase into a malonyl-CoA decarboxylase. Under certain conditons the iodoacetamide-treated enzyme could catalyse 6-methylsalicylic acid formation from malonyl CoA and NADPH without the external addition of acetyl CoA.", "contents": "6-Methylsalicylic acid synthetase from Penicillium patulum. Some catalytic properties of the enzyme and its relation to fatty acid synthetase. 1. The specificity of 6-methylsalicylic acid synthetase with respect to the priming substrate was studied. If acetyl-CoA was replaced by propionyl-CoA 6-ethylsalicylic acid was synthesized. The rate of this synthesis was about 13% that of 6-methylsalicylic acid synthesis in presence of acetyl-CoA. 2. 6-Methylsalicylic acid synthetase contains an acetyl transferase activity as demonstrated by the transfer of acetyl residues from acetyl-CoA to pantetheine. This transferase also catalyses propionyl transfer but only with an about 13 times slower rate. 3. Analogous to fatty acid synthetase, treatment with iodoacetamide converts 6-methylsalicylic acid synthetase into a malonyl-CoA decarboxylase. Under certain conditons the iodoacetamide-treated enzyme could catalyse 6-methylsalicylic acid formation from malonyl CoA and NADPH without the external addition of acetyl CoA.", "PMID": 976275}
{"id": "pubmed23n0032_10637", "title": "The metabolism of O-acyl-N-acylneuraminic acids. Biosynthesis of O-acylated sialic acids in bovine and equine submandibular glands.", "content": "1. The enzymic synthesis of 4-O-acetylneuraminic acid, 4-O-acetyl-N-glycolyneuraminic acid, 4-O-glycolyl-N-acetylneuraminic acid, 9-O-acetyl-N-acetylneuraminic acid and 9-O-acetyl-N-glycolyneuraminic acid is shown using radioactive precursors with surviving slices, membrane fractions or particle-free homogenates from bovine and equine submandibular glands. 2. Acetyl-CoA: N-acetylneuraminate-9(or 7)-O-acetyltransferase activity was found in a microsome fraction and in the cytosol of bovine submandibular glands. The properties of the membrane-bound enzyme acting on endogenous, glycoprotein-bound N-acetyl- and N-glycolylneuraminic acids were compared with those of the soluble enzyme, O-acetylating exogenous, non-glycosidically bound N-acetyl- and N-glycolyneuraminic acids. 3. A rapid, radioactive assay for the membrane-bound enzyme activity is described. The enzyme activity shows an optimum at pH 7 and has  a Km for acetyl-CoA of 0.1 mM. The enzyme is inhibited by p-chloromercuribenzoate and iodoacetate. Divalent cations, EDTA and glutathione have no influence on its activity while CoA proved to be a competitive inhibitor with a Ki of 0.56 mM. 4. The soluble enzyme activity, assayed using a radioactive procedure, shows Km values of 0.01 mM, 0.5 mM and 0.39 mM for acetyl-CoA, N-acetylneuraminic acid and N-glycolylneuraminic acid respectively. The general properties are similar to those found for the membrane-bound enzyme, except that membrane-bound activity is stable for longer on storage at 4 degrees C. 5. Acetyl-CoA, acyl-CoA and CoA concentrations of 33 nmol, 65 nmol and 106 nmol/g wet tissue respectively are found in fresh bovine submandibular glands. 6. The occurrence of the CMP-glycosides of N-acetylneuraminic acid, 9-O-acetyl-N-acetyl-neuraminic acid and N-glycolylneuraminic acid in bovine submandibular glands is demonstrated. 7. The results are discussed in relation to the general metabolism of acylneuraminic acids.", "contents": "The metabolism of O-acyl-N-acylneuraminic acids. Biosynthesis of O-acylated sialic acids in bovine and equine submandibular glands. 1. The enzymic synthesis of 4-O-acetylneuraminic acid, 4-O-acetyl-N-glycolyneuraminic acid, 4-O-glycolyl-N-acetylneuraminic acid, 9-O-acetyl-N-acetylneuraminic acid and 9-O-acetyl-N-glycolyneuraminic acid is shown using radioactive precursors with surviving slices, membrane fractions or particle-free homogenates from bovine and equine submandibular glands. 2. Acetyl-CoA: N-acetylneuraminate-9(or 7)-O-acetyltransferase activity was found in a microsome fraction and in the cytosol of bovine submandibular glands. The properties of the membrane-bound enzyme acting on endogenous, glycoprotein-bound N-acetyl- and N-glycolylneuraminic acids were compared with those of the soluble enzyme, O-acetylating exogenous, non-glycosidically bound N-acetyl- and N-glycolyneuraminic acids. 3. A rapid, radioactive assay for the membrane-bound enzyme activity is described. The enzyme activity shows an optimum at pH 7 and has  a Km for acetyl-CoA of 0.1 mM. The enzyme is inhibited by p-chloromercuribenzoate and iodoacetate. Divalent cations, EDTA and glutathione have no influence on its activity while CoA proved to be a competitive inhibitor with a Ki of 0.56 mM. 4. The soluble enzyme activity, assayed using a radioactive procedure, shows Km values of 0.01 mM, 0.5 mM and 0.39 mM for acetyl-CoA, N-acetylneuraminic acid and N-glycolylneuraminic acid respectively. The general properties are similar to those found for the membrane-bound enzyme, except that membrane-bound activity is stable for longer on storage at 4 degrees C. 5. Acetyl-CoA, acyl-CoA and CoA concentrations of 33 nmol, 65 nmol and 106 nmol/g wet tissue respectively are found in fresh bovine submandibular glands. 6. The occurrence of the CMP-glycosides of N-acetylneuraminic acid, 9-O-acetyl-N-acetyl-neuraminic acid and N-glycolylneuraminic acid in bovine submandibular glands is demonstrated. 7. The results are discussed in relation to the general metabolism of acylneuraminic acids.", "PMID": 976276}
{"id": "pubmed23n0032_10638", "title": "A new familial intrauterine growth retardation syndrome the \"3-M syndrome\".", "content": "Two pairs of siblings are described with proportionate dwarfism due to skeletal hypoplasia of prenatal onset. The head size was normal for age and disproportionately large for height. The patients had a characteristic face different from that seen in the Silver-Russell syndrome. The family data are in accordance with autosomal recessive inheritance. In spite of some similarities, the bulk of clinical and genetic evidence suggests that the described intrauterine growth retardation syndrome is different from the Silver-Russell syndrome and presents an apparently \"new\" entity which has been designated 3-M syndrome.", "contents": "A new familial intrauterine growth retardation syndrome the \"3-M syndrome\". Two pairs of siblings are described with proportionate dwarfism due to skeletal hypoplasia of prenatal onset. The head size was normal for age and disproportionately large for height. The patients had a characteristic face different from that seen in the Silver-Russell syndrome. The family data are in accordance with autosomal recessive inheritance. In spite of some similarities, the bulk of clinical and genetic evidence suggests that the described intrauterine growth retardation syndrome is different from the Silver-Russell syndrome and presents an apparently \"new\" entity which has been designated 3-M syndrome.", "PMID": 976277}
{"id": "pubmed23n0032_10639", "title": "Latex phagocytosis by polymorphonuclear leukocytes. In vitro and in vivo studies with a simple screening test.", "content": "A simple semiquantitative slide test to assess the phagocytic activity of periphera polymorphonuclear leukocytes is described. Leukocytes are isolated from one drop of blood and latex particles are offered for phagocytosis. Cellular capacities and serum opsonins can be assessed separately. In the presence of pooled adult serum no defect of phagocytosis was seen in preterm and full-term newborns nor in pediatric patients with infectious or other diseases. In 4 of 7 premature newborns a definite deficiency of serum opsonins was demonstrated. In in vitro studies complement (plus possibly other heat-labile factors) promoted phagocytosis. Phagocytic rates were depressed by endotoxin and phenylbutazone.", "contents": "Latex phagocytosis by polymorphonuclear leukocytes. In vitro and in vivo studies with a simple screening test. A simple semiquantitative slide test to assess the phagocytic activity of periphera polymorphonuclear leukocytes is described. Leukocytes are isolated from one drop of blood and latex particles are offered for phagocytosis. Cellular capacities and serum opsonins can be assessed separately. In the presence of pooled adult serum no defect of phagocytosis was seen in preterm and full-term newborns nor in pediatric patients with infectious or other diseases. In 4 of 7 premature newborns a definite deficiency of serum opsonins was demonstrated. In in vitro studies complement (plus possibly other heat-labile factors) promoted phagocytosis. Phagocytic rates were depressed by endotoxin and phenylbutazone.", "PMID": 976278}
{"id": "pubmed23n0032_10640", "title": "Analysis of etiologic factors in cerebral palsy with severe mental retardation. I. Analysis of gestational, parturitional and neonatal data.", "content": "We analyzed the gestational, parturitional, neonatal (GPN) histories of 281 severely mentally retarded patients with cerebral palsy to define the etiology or pathogenesis of cerebral palsy in each patient. No association between type of cerebral palsy and GPN histories was found except for an increase in spastic-athetoid patients in the breech delivery subgroup. Significant findings include: increased incidence of prematurity and postmaturity, small and large for-gestational age (GA) fetal size, a normal birthweight for GA distribution of patients with diabetic mothers, an excess of mothers greater than or equal to 35 and less than or equal to 20 years old, an increased immediately-previous sib interval of 2.59 years suggestive of an \"infertility factor\", an unremarkable GPN history in one third of the cases, in another one third GPN problems not usually associated with a high risk of CNS damage, and in one third gross complications which were probably responsible for the CP, including: an increased incidence of breech deliveries, twinning, prolonged and precipitous labor and placental complications; no increased association of athetosis and Rh incompatibility or incidence of toxemia was found. Disseminated intravascular coagulation due to prenatal death of a twin may have been the cause of brain damage in several patients.", "contents": "Analysis of etiologic factors in cerebral palsy with severe mental retardation. I. Analysis of gestational, parturitional and neonatal data. We analyzed the gestational, parturitional, neonatal (GPN) histories of 281 severely mentally retarded patients with cerebral palsy to define the etiology or pathogenesis of cerebral palsy in each patient. No association between type of cerebral palsy and GPN histories was found except for an increase in spastic-athetoid patients in the breech delivery subgroup. Significant findings include: increased incidence of prematurity and postmaturity, small and large for-gestational age (GA) fetal size, a normal birthweight for GA distribution of patients with diabetic mothers, an excess of mothers greater than or equal to 35 and less than or equal to 20 years old, an increased immediately-previous sib interval of 2.59 years suggestive of an \"infertility factor\", an unremarkable GPN history in one third of the cases, in another one third GPN problems not usually associated with a high risk of CNS damage, and in one third gross complications which were probably responsible for the CP, including: an increased incidence of breech deliveries, twinning, prolonged and precipitous labor and placental complications; no increased association of athetosis and Rh incompatibility or incidence of toxemia was found. Disseminated intravascular coagulation due to prenatal death of a twin may have been the cause of brain damage in several patients.", "PMID": 976279}
{"id": "pubmed23n0032_10641", "title": "Serum lactate dehydrogenase isoenzyme pattern in neuroblastoma.", "content": "Total serum LDH activity and isoenzyme distribution were studied in children with neuroblastoma at the time of hospital admission. The total LDH was determined in 26 cases, and 20 (77%) of them showed elevation of its activity. On the other hand, in 9 of these 26 cases, the isoenzyme distribution was determined along with the total LDH. All 9 cases, 4 of them with normal total LDH activity, showed an abnormal isoenzyme pattern with a percentage increase in the intermediate fractions (malignant pattern). The results suggest the usefulness of the determination of serum LDH isoenzymes as a screening procedure in children with malignant tumors including neuroblastoma.", "contents": "Serum lactate dehydrogenase isoenzyme pattern in neuroblastoma. Total serum LDH activity and isoenzyme distribution were studied in children with neuroblastoma at the time of hospital admission. The total LDH was determined in 26 cases, and 20 (77%) of them showed elevation of its activity. On the other hand, in 9 of these 26 cases, the isoenzyme distribution was determined along with the total LDH. All 9 cases, 4 of them with normal total LDH activity, showed an abnormal isoenzyme pattern with a percentage increase in the intermediate fractions (malignant pattern). The results suggest the usefulness of the determination of serum LDH isoenzymes as a screening procedure in children with malignant tumors including neuroblastoma.", "PMID": 976280}
{"id": "pubmed23n0032_10642", "title": "A genetic and statistical study of the respiratory distress syndrome.", "content": "The hospital records of 197 infants with the respiratory distress syndrome (RDS) were reviewed and the families of 111 of them subsequently contacted to obtain a family history. After correcting for biasis of ascertainment, the incidence of RDS among the full sibs was found to be between 12 and 19% depending on whether the individuals diagnosed as \"possible RDS\" were counted as affected. Among the low birth weight (LBW, less than or equal to 2.5 kg) and/or preterm (less than or equal to 37 weeks gestation) infants in the sibships, the incidence of RDS was 32-50%. Considering only sibs born after the probands yielded the empiric recurrence risk of 17--27% for all younger sibs and 39--67% for LBW/preterm younger sibs. The risk for maternal half-sibs was of about the same magnitude as that for full sibs, while the risk for paternal half-sibs was minimal. Among the LBW/preterm first cousins of probands, only the infants of maternal aunts showed an RDS incidence clearly higher than that in the general population. We think these data suggest a genetically determined maternal factor predisposing the infants of certain mothers to RDS. Other significant findings include: 1) an excess of males among the probands but a normal sex ratio among the sibs of the probands; 2) a decrease in mean birth weight and mean length of gestation for not only the probands but also their sibs; 3) a decrease in the mean parental ages at the birth of the probands; 4) a relative dearth of first-born and an excess of second-born infants among the probands; 5) an increased incidence of stillbirths in the sibships; 6) an increased number of probands born by cesarean section; and 7) a twin concordance of 75%.", "contents": "A genetic and statistical study of the respiratory distress syndrome. The hospital records of 197 infants with the respiratory distress syndrome (RDS) were reviewed and the families of 111 of them subsequently contacted to obtain a family history. After correcting for biasis of ascertainment, the incidence of RDS among the full sibs was found to be between 12 and 19% depending on whether the individuals diagnosed as \"possible RDS\" were counted as affected. Among the low birth weight (LBW, less than or equal to 2.5 kg) and/or preterm (less than or equal to 37 weeks gestation) infants in the sibships, the incidence of RDS was 32-50%. Considering only sibs born after the probands yielded the empiric recurrence risk of 17--27% for all younger sibs and 39--67% for LBW/preterm younger sibs. The risk for maternal half-sibs was of about the same magnitude as that for full sibs, while the risk for paternal half-sibs was minimal. Among the LBW/preterm first cousins of probands, only the infants of maternal aunts showed an RDS incidence clearly higher than that in the general population. We think these data suggest a genetically determined maternal factor predisposing the infants of certain mothers to RDS. Other significant findings include: 1) an excess of males among the probands but a normal sex ratio among the sibs of the probands; 2) a decrease in mean birth weight and mean length of gestation for not only the probands but also their sibs; 3) a decrease in the mean parental ages at the birth of the probands; 4) a relative dearth of first-born and an excess of second-born infants among the probands; 5) an increased incidence of stillbirths in the sibships; 6) an increased number of probands born by cesarean section; and 7) a twin concordance of 75%.", "PMID": 976282}
{"id": "pubmed23n0032_10643", "title": "Prenatal diagnosis of osteogenesis imperfecta congenita by means of fetography.", "content": "By means of fetography, prenatal diagnosis of osteogenesis imperfecta congenita was performed on two pregnant women in the 34th week of gestation with familial histories of the disease. In 1 of the 2 cases, the fetogram revealed abnormal twisting of the torso, shortening, and severe angulation deformities of the extremities with callus formation and parchmentlike skull despite clear-cut outline of the scalp. On the basis of these findings, the fetus was diagnosed as osteogenesis imperfecta congenita and subsequently confirmed. Thus, the excellent fetal resolving power of fetography permitted successful prenatal diagnosis of the disease far more easily and precisely than plain roentgenography.", "contents": "Prenatal diagnosis of osteogenesis imperfecta congenita by means of fetography. By means of fetography, prenatal diagnosis of osteogenesis imperfecta congenita was performed on two pregnant women in the 34th week of gestation with familial histories of the disease. In 1 of the 2 cases, the fetogram revealed abnormal twisting of the torso, shortening, and severe angulation deformities of the extremities with callus formation and parchmentlike skull despite clear-cut outline of the scalp. On the basis of these findings, the fetus was diagnosed as osteogenesis imperfecta congenita and subsequently confirmed. Thus, the excellent fetal resolving power of fetography permitted successful prenatal diagnosis of the disease far more easily and precisely than plain roentgenography.", "PMID": 976283}
{"id": "pubmed23n0032_10644", "title": "Studies of malformation syndromes of man XXIX: the Wiedemann-Beckwith syndrome. Clinical, genetic and pathogenetic studies of 12 cases.", "content": "This report describes 12 patients with the Wiedemann-Beckwith syndrome (WBS), including 6 familial cases from 2 families. The clinical manifestations do not allow for a differentiation between familial and sporadic cases. Consistent morphologic features include organomegaly, cytomegaly and nucleomegaly. The pathogenetic process may involve few or many organs and tissues and may represent a nuclear/mitotic dysfunction. Clinically, the manifestations are hyperplasia, hypoplasia, dysplasia, neoplasia and defects in differentiation. Secondary functional disturbances are at times prominent. The differential diagnosis of the WBS includes 1) the Wilm's tumor (WT)-aniridia syndrome: 2) the \"tumor-hypertrophy syndrome\" which includes WT, adenocortical tumors or hepatoblastoma; 3) the WT-pseudohermaphroditism syndrome; and 4) the \"tumor-nevus syndrome\" with or without malformations (particularly duplications) of the urinary tract. The latter two conditions are apparently not associated with hemihypertrophy. Familial occurrence suggests that some cases of the WBS may be due to delayed mutation. Carriers of the premutated allele appear to belong to two classes: those with a high risk of producing affected offspring and those who transmit the premutated allele but have no affected offspring.", "contents": "Studies of malformation syndromes of man XXIX: the Wiedemann-Beckwith syndrome. Clinical, genetic and pathogenetic studies of 12 cases. This report describes 12 patients with the Wiedemann-Beckwith syndrome (WBS), including 6 familial cases from 2 families. The clinical manifestations do not allow for a differentiation between familial and sporadic cases. Consistent morphologic features include organomegaly, cytomegaly and nucleomegaly. The pathogenetic process may involve few or many organs and tissues and may represent a nuclear/mitotic dysfunction. Clinically, the manifestations are hyperplasia, hypoplasia, dysplasia, neoplasia and defects in differentiation. Secondary functional disturbances are at times prominent. The differential diagnosis of the WBS includes 1) the Wilm's tumor (WT)-aniridia syndrome: 2) the \"tumor-hypertrophy syndrome\" which includes WT, adenocortical tumors or hepatoblastoma; 3) the WT-pseudohermaphroditism syndrome; and 4) the \"tumor-nevus syndrome\" with or without malformations (particularly duplications) of the urinary tract. The latter two conditions are apparently not associated with hemihypertrophy. Familial occurrence suggests that some cases of the WBS may be due to delayed mutation. Carriers of the premutated allele appear to belong to two classes: those with a high risk of producing affected offspring and those who transmit the premutated allele but have no affected offspring.", "PMID": 976281}
{"id": "pubmed23n0032_10645", "title": "In vitro studies on the way in which the activation of factor VIII is affected in mixtures of plasma with hemophilia A plasma.", "content": "Factor VIII exchange test experiments with hemophilia A plasmas were performed to find out how the results were affected by submitting plasmas and plasma mixtures to different incubation periods at 37 degrees C, heat precipitation, and ether extraction. The experiments led to the following results: 1. In plasma mixtures, frequently higher factor VIII activities are found than can be expected from the single activities of the used plasmas. Activity increases are factor-specific. 2. The component to be activated is in the hemophilic plasma; the activity-increasing agent is in the normal plasma. These results lead to the hypothesis that hemophilia A patients have sufficient quantities of inactive factor VIII, but it stays inactive for lack of the necessary activator. In normal plasma the activator is in balance or surplus to the inactive factor. By adding normal plasma, thus supplying free activator, the inactive factor VIII of hemophilia A plasma can, under suitable conditions, be developed into active factor VIII.", "contents": "In vitro studies on the way in which the activation of factor VIII is affected in mixtures of plasma with hemophilia A plasma. Factor VIII exchange test experiments with hemophilia A plasmas were performed to find out how the results were affected by submitting plasmas and plasma mixtures to different incubation periods at 37 degrees C, heat precipitation, and ether extraction. The experiments led to the following results: 1. In plasma mixtures, frequently higher factor VIII activities are found than can be expected from the single activities of the used plasmas. Activity increases are factor-specific. 2. The component to be activated is in the hemophilic plasma; the activity-increasing agent is in the normal plasma. These results lead to the hypothesis that hemophilia A patients have sufficient quantities of inactive factor VIII, but it stays inactive for lack of the necessary activator. In normal plasma the activator is in balance or surplus to the inactive factor. By adding normal plasma, thus supplying free activator, the inactive factor VIII of hemophilia A plasma can, under suitable conditions, be developed into active factor VIII.", "PMID": 976284}
{"id": "pubmed23n0032_10646", "title": "Comparative studies on coagulation activities in normal plasmas and their mixtures.", "content": "By single factor exchange test experiments it was proved that mixtures of normal plasmas usually have the coagulation activity that can be concluded from the single activities of the used plasmas. In individual cases, however, normal plasmas influence each other in the mixture to the effect that coagulation is increased or decreased. This has to be considered when plasma pools for standard curves are established.", "contents": "Comparative studies on coagulation activities in normal plasmas and their mixtures. By single factor exchange test experiments it was proved that mixtures of normal plasmas usually have the coagulation activity that can be concluded from the single activities of the used plasmas. In individual cases, however, normal plasmas influence each other in the mixture to the effect that coagulation is increased or decreased. This has to be considered when plasma pools for standard curves are established.", "PMID": 976285}
{"id": "pubmed23n0032_10647", "title": "Intestinal adaptation after jejuno-ileal bypass operation for massive obesity.", "content": "Intestinal adaptation was studied in six patients with massive obesity treated by jejuno-ileal bypass operation. Glucose absorption in the jejunum was measured by a perfusion technique. The morphometric and enzymatic measurements were carried out on biopsies from the proximal jejunum and the distal ileum. Results obtained before and six months after the operation were compared. The glucose absorption per unit length of jejunum was unchanged at a glucose concentration of 66 mmol/l in the perfusate but increased significantly at a glucose concentration of 133 mmol/l (p less 0.025). The mean sucrase activity did not change, whereas the lactase activity increased significantly in the jejunum and ileum. The mean villus height increased significantly, while the epithelial cell height and cell width were unchanged both in the jejunum and the ileum, suggesting that the operation resulted in epithelial cell hyperplasia. The glucose absorption in the jejunum was positively correlated with the villus height (r = 0.76), which suggests that the increased glucose absorption was related to an increased number of epithelial cells.", "contents": "Intestinal adaptation after jejuno-ileal bypass operation for massive obesity. Intestinal adaptation was studied in six patients with massive obesity treated by jejuno-ileal bypass operation. Glucose absorption in the jejunum was measured by a perfusion technique. The morphometric and enzymatic measurements were carried out on biopsies from the proximal jejunum and the distal ileum. Results obtained before and six months after the operation were compared. The glucose absorption per unit length of jejunum was unchanged at a glucose concentration of 66 mmol/l in the perfusate but increased significantly at a glucose concentration of 133 mmol/l (p less 0.025). The mean sucrase activity did not change, whereas the lactase activity increased significantly in the jejunum and ileum. The mean villus height increased significantly, while the epithelial cell height and cell width were unchanged both in the jejunum and the ileum, suggesting that the operation resulted in epithelial cell hyperplasia. The glucose absorption in the jejunum was positively correlated with the villus height (r = 0.76), which suggests that the increased glucose absorption was related to an increased number of epithelial cells.", "PMID": 976297}
{"id": "pubmed23n0032_10648", "title": "Peptide-bound hydroxylysine and large polypeptides related to collagen synthesis.", "content": "About 10% of the urinary hydroxylysine is linked to peptides. There is no significant difference between the excretion of peptide-bound hydroxylysine and hydroxylysine measured in the non-dialysable fraction of the urine. Non-dialysable hydroxylysine is highly glycosylated and contains mainly glucosyl-galactosyl-hydroxylysine. It is concluded that the urinary peptide-bound hydroxylysine is present in the non-dialysable collagen polypeptides. Their unusual carbohydrate composition is discussed.", "contents": "Peptide-bound hydroxylysine and large polypeptides related to collagen synthesis. About 10% of the urinary hydroxylysine is linked to peptides. There is no significant difference between the excretion of peptide-bound hydroxylysine and hydroxylysine measured in the non-dialysable fraction of the urine. Non-dialysable hydroxylysine is highly glycosylated and contains mainly glucosyl-galactosyl-hydroxylysine. It is concluded that the urinary peptide-bound hydroxylysine is present in the non-dialysable collagen polypeptides. Their unusual carbohydrate composition is discussed.", "PMID": 976298}
{"id": "pubmed23n0032_10649", "title": "Effect of lead on hepatic delta-aminolaevulinic acid synthetase activity in the rat: a model for drug sensitivity in intermittent acute porphyria.", "content": "The hereditary hepatic porphyrias are disorders of porphyrin and haem synthesis characterized by a marked idiosyncrasy towards a variety of lipid soluble drugs. Most of these agents are inducers of the haemoprotein cytochrome P450, the terminal oxidase in drug metabolism. The primary genetic defect in intermittent acute porphyria is a partial deficiency of uroporphyrinogen I synthetase, which may result in a secondary derepression of delta-aminoaevulinic acid synthetase, the rate-limiting enzyme in the haem pathway. Analogous defects at more distant sites may explain the other hereditary hepatic porphyrias. As drug sensitivity may be related to the defect in haem synthesis, we investigated the effects of experimental partial blocks in haem synthesis produced by lead in rats. Drug effects on delta-aminolaevulinic acid synthetase, cytochrome P450, And drug metabolism were studied. Our findings indicate: a) While partial impairment of haem biosynthesis has only minor effects on delta-aminolaevulinic acid synthetase activity, it greatly enhances the sensitivity of delta-aminolaevulinic acid synthetase to induction by drugs and steroids, which when given alone, have little or no inducing effect on the enzyme. b) The experimental partial block in haem synthesis delays and impairs drug-mediated induction cytochrome P450 and drug metabolism in vitro. The findings may explain why a large number of structurally unrelated compounds with little effect on normal liver can precipitate \"aucte porphyria\".", "contents": "Effect of lead on hepatic delta-aminolaevulinic acid synthetase activity in the rat: a model for drug sensitivity in intermittent acute porphyria. The hereditary hepatic porphyrias are disorders of porphyrin and haem synthesis characterized by a marked idiosyncrasy towards a variety of lipid soluble drugs. Most of these agents are inducers of the haemoprotein cytochrome P450, the terminal oxidase in drug metabolism. The primary genetic defect in intermittent acute porphyria is a partial deficiency of uroporphyrinogen I synthetase, which may result in a secondary derepression of delta-aminoaevulinic acid synthetase, the rate-limiting enzyme in the haem pathway. Analogous defects at more distant sites may explain the other hereditary hepatic porphyrias. As drug sensitivity may be related to the defect in haem synthesis, we investigated the effects of experimental partial blocks in haem synthesis produced by lead in rats. Drug effects on delta-aminolaevulinic acid synthetase, cytochrome P450, And drug metabolism were studied. Our findings indicate: a) While partial impairment of haem biosynthesis has only minor effects on delta-aminolaevulinic acid synthetase activity, it greatly enhances the sensitivity of delta-aminolaevulinic acid synthetase to induction by drugs and steroids, which when given alone, have little or no inducing effect on the enzyme. b) The experimental partial block in haem synthesis delays and impairs drug-mediated induction cytochrome P450 and drug metabolism in vitro. The findings may explain why a large number of structurally unrelated compounds with little effect on normal liver can precipitate \"aucte porphyria\".", "PMID": 976299}
{"id": "pubmed23n0032_10650", "title": "Plasma renin activity in diabetic autonomic neuropathy.", "content": "Postural changes in plasma renin activity were studied in three groups of age and duration-matched male diabetics (potent, impotent and with postural hypotension) and in non-diabetic control subjects. Those diabetic subjects with postural hypotension due to automatic neuropathy had no increase in plasma renin activity to the erect posture whereas both the potent and impotent groups had similar plasma renin activity responses to the control subjects. There was a significant inverse correlation between the rise in plasma renin activity on standing and the postural drop in blood pressure (r = 0.476, P less than 0.01) but no correlation with other tests of autonomic reflex function such as the Valsalva manoeuvre and blood pressure response to sustained handgrip. The results suggested that the lesion responsible for the postural hypotension is in the efferent sympathetic pathway. However, neuropathy per se did not wholly explain the decreased postural plasma renin activity response. Diabetic nephropathy, with involvement of cells of juxtaglomerular apparatus, may also be implicated.", "contents": "Plasma renin activity in diabetic autonomic neuropathy. Postural changes in plasma renin activity were studied in three groups of age and duration-matched male diabetics (potent, impotent and with postural hypotension) and in non-diabetic control subjects. Those diabetic subjects with postural hypotension due to automatic neuropathy had no increase in plasma renin activity to the erect posture whereas both the potent and impotent groups had similar plasma renin activity responses to the control subjects. There was a significant inverse correlation between the rise in plasma renin activity on standing and the postural drop in blood pressure (r = 0.476, P less than 0.01) but no correlation with other tests of autonomic reflex function such as the Valsalva manoeuvre and blood pressure response to sustained handgrip. The results suggested that the lesion responsible for the postural hypotension is in the efferent sympathetic pathway. However, neuropathy per se did not wholly explain the decreased postural plasma renin activity response. Diabetic nephropathy, with involvement of cells of juxtaglomerular apparatus, may also be implicated.", "PMID": 976300}
{"id": "pubmed23n0032_10651", "title": "The role of the beta-adrnergic receptor in the secretion of gastrin: studies in normal subjects and in patients with duodenal ulcers.", "content": "Intravenous infusion of isoproterenol, a beta-adrenergic receptor stimulatory agent, increased serum gastrin concentration significantly more in patients with a duodenal ulcer than in healthy subjects. The rise in pulse rate, blood glucose concentration and in serum insulin was the same in both groups of subjects. Gastrin secretion was also increased significantly more in the patients than in the control subjects after a beef-meal. Basal serum gastrin concentrations were higher in the patients than in the control subjects and correlated to the rise in serum gastrin during both tests in the patients with a duodenal ulcer. Isoproterenol and meal stimulated gastrin secretion, expressed as percent of the basal value, were twice as higher in the patients as in the control subjects. The combined administration of isoproterenol and the meal had an additive effect on the rise in serum gastrin. Isoproterenol stimulated gastrin secretion was completely suppressed by propranolol, a beta-adrenergic receptor blocking agent, which had no effect on meal stimulated gastrin secretion. It is concluded that the mechanism of the hypersecretion of gastrin in patients with a duodenal ulcer did not involve a specific abnormality of the beta-adrenergic receptor or the receptor which recognized proteins and their digested products. There is no established role of beta-adrenergic receptor activity in the hypersecretion of gastrin in patients with duodenal ulcers. It is suggested that the beta-adrenergic receptor may have some yet unknown function unrelated to the acute secretory response of gastrin.", "contents": "The role of the beta-adrnergic receptor in the secretion of gastrin: studies in normal subjects and in patients with duodenal ulcers. Intravenous infusion of isoproterenol, a beta-adrenergic receptor stimulatory agent, increased serum gastrin concentration significantly more in patients with a duodenal ulcer than in healthy subjects. The rise in pulse rate, blood glucose concentration and in serum insulin was the same in both groups of subjects. Gastrin secretion was also increased significantly more in the patients than in the control subjects after a beef-meal. Basal serum gastrin concentrations were higher in the patients than in the control subjects and correlated to the rise in serum gastrin during both tests in the patients with a duodenal ulcer. Isoproterenol and meal stimulated gastrin secretion, expressed as percent of the basal value, were twice as higher in the patients as in the control subjects. The combined administration of isoproterenol and the meal had an additive effect on the rise in serum gastrin. Isoproterenol stimulated gastrin secretion was completely suppressed by propranolol, a beta-adrenergic receptor blocking agent, which had no effect on meal stimulated gastrin secretion. It is concluded that the mechanism of the hypersecretion of gastrin in patients with a duodenal ulcer did not involve a specific abnormality of the beta-adrenergic receptor or the receptor which recognized proteins and their digested products. There is no established role of beta-adrenergic receptor activity in the hypersecretion of gastrin in patients with duodenal ulcers. It is suggested that the beta-adrenergic receptor may have some yet unknown function unrelated to the acute secretory response of gastrin.", "PMID": 976301}
{"id": "pubmed23n0032_10652", "title": "Plasma and urinary dopamine: studies during fasting and exercise and in tetraplegic man.", "content": "Dopamine, noradrenaline and adrenaline were measured in plasma and in urine, using double-isotope derivative techniques, in 46 normal subjects and in 17 tetraplegic patients with physiologically complete cervical spinal cord transections above the sympathetic outflow. Dopamine was present in plasma in normal subjects in a concentration of 0.33 mug/l +/- 0.06 (SEM). Twenty-four hour urinary excretion of dopamine averaged 248 mug +/- 22. There was a significant correlation between the 24 h urinary excretion of dopamine and of noradrenaline. In the normal subjects plasma dopamine and the urinary excretion of dopamine did not change during three days of fasting while urinary excretion of adrenaline increased twofold. In the normal subjects exercise significantly increased plasma dopamine from 0.25 mug/l to 0.43 mug/l, but significantly decreased the urinary excretion of dopamine. Exercise significantly increased the excretion of noradrenaline. In the tetraplegic patients the plasma dopamine concentration and the urinary excretion of dopamine were lower but not significantly different from the corresponding values in the normal subjects. Plasma noradrenaline and the urinary excretion of noradrenaline and adrenaline were significantly lower in the tetraplegic patients. It is concluded that dopamine is present in human plasma in concentrations similar to that of noradrenaline. Free dopamine in plasma and urine of normal subjects is not dependent on foot intake. Urinary dopamine may be derived from circulating dopamine. Urinary dopamine does not necessarily appear to reflect changes in plasma dopamine. The relationship between plasma dopamine and changes in adrenergic nervous activity deserves further investigation.", "contents": "Plasma and urinary dopamine: studies during fasting and exercise and in tetraplegic man. Dopamine, noradrenaline and adrenaline were measured in plasma and in urine, using double-isotope derivative techniques, in 46 normal subjects and in 17 tetraplegic patients with physiologically complete cervical spinal cord transections above the sympathetic outflow. Dopamine was present in plasma in normal subjects in a concentration of 0.33 mug/l +/- 0.06 (SEM). Twenty-four hour urinary excretion of dopamine averaged 248 mug +/- 22. There was a significant correlation between the 24 h urinary excretion of dopamine and of noradrenaline. In the normal subjects plasma dopamine and the urinary excretion of dopamine did not change during three days of fasting while urinary excretion of adrenaline increased twofold. In the normal subjects exercise significantly increased plasma dopamine from 0.25 mug/l to 0.43 mug/l, but significantly decreased the urinary excretion of dopamine. Exercise significantly increased the excretion of noradrenaline. In the tetraplegic patients the plasma dopamine concentration and the urinary excretion of dopamine were lower but not significantly different from the corresponding values in the normal subjects. Plasma noradrenaline and the urinary excretion of noradrenaline and adrenaline were significantly lower in the tetraplegic patients. It is concluded that dopamine is present in human plasma in concentrations similar to that of noradrenaline. Free dopamine in plasma and urine of normal subjects is not dependent on foot intake. Urinary dopamine may be derived from circulating dopamine. Urinary dopamine does not necessarily appear to reflect changes in plasma dopamine. The relationship between plasma dopamine and changes in adrenergic nervous activity deserves further investigation.", "PMID": 976302}
{"id": "pubmed23n0032_10653", "title": "Haemodynamic effects of procainamide and phenytoin at apparent therapeutic plasma levels.", "content": "The haemodynamic effects of procainamide and phenytoin after intravenous administration to 19 patients with suspected congenital heart lesions were studied. The drugs were injected into the pulmonary artery in a dose of 5 mg per kg bodyweight, at an infusion rate of 50 mg per minute, thus giving plasma concentrations within the suggested therapeutic ranges. The diastolic pressures in the pulmonary artery and the right ventricle were reduced by procainamide and the converse effect was noted after phenytoin. The heart rate was significantly increased by both drugs but with phenytoin this effect was only transitory. The latter drug also caused a small and transient fall in cardiac output and mean arterial pressure. It was concluded that of the two drugs phenytoin seemed to have the most marked myocardial depressant effect.", "contents": "Haemodynamic effects of procainamide and phenytoin at apparent therapeutic plasma levels. The haemodynamic effects of procainamide and phenytoin after intravenous administration to 19 patients with suspected congenital heart lesions were studied. The drugs were injected into the pulmonary artery in a dose of 5 mg per kg bodyweight, at an infusion rate of 50 mg per minute, thus giving plasma concentrations within the suggested therapeutic ranges. The diastolic pressures in the pulmonary artery and the right ventricle were reduced by procainamide and the converse effect was noted after phenytoin. The heart rate was significantly increased by both drugs but with phenytoin this effect was only transitory. The latter drug also caused a small and transient fall in cardiac output and mean arterial pressure. It was concluded that of the two drugs phenytoin seemed to have the most marked myocardial depressant effect.", "PMID": 976303}
{"id": "pubmed23n0032_10654", "title": "Studies on hydralazine. II. Elimination rate and steady-state concentration in patients with impaired renal function.", "content": "Following a single 50 mg dose of hydralazine (Apresoline) in 13 patients with impaired renal function, a decrease in glomerular filtration rate (GFR) was correlated with an increase in serum half-life (T1/2) of the drug (r=-0.69; p less than 0.01). The T1/2 was 15.8 h in one patient with a GFR of 16 ml-min-1, as compared to a T1/2 of 1.7-3.0 h found previously in 16 healthy volunteers. In 49 patients on long-term antihypertensive treatment with hydralazine, the ratio between the minimum steady-state drug concentration and the daily dose of hydralazine (Cminss : Dose) increased as the GFR decreased. This accumulation of the drug was particularly evident in patients with a GFR less than 30 ml-min-1 (r=-0.63; p less than 0.01; n=19). As renal excretion of unchanged hydralazine is generally regarded as unimportant, the slower elimination rate in chronic renal failure was probably caused by a slower rate of metabolic conversion. It was found, however, that the renal excretion of hydralazine could easily have been underestimated, as only 12.7% of an initial hydralazine concentration of 200 ng-ml-1 in urine could be recovered after storage of the samples at room temperature for 24 h.", "contents": "Studies on hydralazine. II. Elimination rate and steady-state concentration in patients with impaired renal function. Following a single 50 mg dose of hydralazine (Apresoline) in 13 patients with impaired renal function, a decrease in glomerular filtration rate (GFR) was correlated with an increase in serum half-life (T1/2) of the drug (r=-0.69; p less than 0.01). The T1/2 was 15.8 h in one patient with a GFR of 16 ml-min-1, as compared to a T1/2 of 1.7-3.0 h found previously in 16 healthy volunteers. In 49 patients on long-term antihypertensive treatment with hydralazine, the ratio between the minimum steady-state drug concentration and the daily dose of hydralazine (Cminss : Dose) increased as the GFR decreased. This accumulation of the drug was particularly evident in patients with a GFR less than 30 ml-min-1 (r=-0.63; p less than 0.01; n=19). As renal excretion of unchanged hydralazine is generally regarded as unimportant, the slower elimination rate in chronic renal failure was probably caused by a slower rate of metabolic conversion. It was found, however, that the renal excretion of hydralazine could easily have been underestimated, as only 12.7% of an initial hydralazine concentration of 200 ng-ml-1 in urine could be recovered after storage of the samples at room temperature for 24 h.", "PMID": 976304}
{"id": "pubmed23n0032_10655", "title": "Investigation of the pharmacodynamics and pharmacokinetics of 2-(2,4-dimethoxyphenyl)-Imidazo-(4,5-b)-pyridine hydrochloride (AR-L 57 CL) in man.", "content": "AR-L 57 CL is an imidazole derivative which has been shown in animal studies to have a pronounced positive inotropic effect. This effect and the pharmacokinetics of AR-L 57 CL have been investigated by non-invasive methods in 8 healthy volunteers. After a single intravenous dose of 200 mg, administered as part of Phase 1 of the clinical studies, AR-L 57 CL plasma concentrations were measured by fluorimetry at intervals for up to one hour. Its inotropic action on the heart was demonstrated by changes in the systolic time intervals: QS2 = duration of electro-mechanical systole; PEP = pre-ejection period; LVET = left ventricular ejection time. The decrease in plasma concentration could be expressed in terms of an open two-compartment pharmacokinetic model. The shorter elimination phase had a t1/2 of 4 min and the longer a t1/2 of 30 min. Immediately after injection, QS2 and PEP (corrected for heart rate) as well as PEP/LVET (independent of heart rate) decreased considerably. They had returned to normal by 22 min after injection. The plasma concentrations of AR-L 57 CL of 2 - 5 mug-equivalents/ml showed a highly significant correlation with the decrease in systolic time intervals. Both systolic and diastolic blood pressure rose briefly after injection. The AV conduction time fell initially and the heart-rate increased briefly. Thus AR-L 57 CL was shown to be a short acting drug with a high degree of positive inotropic action. It did not cause bradycardia or increase atrioventricular transmission time and appeared to be easily controllable.", "contents": "Investigation of the pharmacodynamics and pharmacokinetics of 2-(2,4-dimethoxyphenyl)-Imidazo-(4,5-b)-pyridine hydrochloride (AR-L 57 CL) in man. AR-L 57 CL is an imidazole derivative which has been shown in animal studies to have a pronounced positive inotropic effect. This effect and the pharmacokinetics of AR-L 57 CL have been investigated by non-invasive methods in 8 healthy volunteers. After a single intravenous dose of 200 mg, administered as part of Phase 1 of the clinical studies, AR-L 57 CL plasma concentrations were measured by fluorimetry at intervals for up to one hour. Its inotropic action on the heart was demonstrated by changes in the systolic time intervals: QS2 = duration of electro-mechanical systole; PEP = pre-ejection period; LVET = left ventricular ejection time. The decrease in plasma concentration could be expressed in terms of an open two-compartment pharmacokinetic model. The shorter elimination phase had a t1/2 of 4 min and the longer a t1/2 of 30 min. Immediately after injection, QS2 and PEP (corrected for heart rate) as well as PEP/LVET (independent of heart rate) decreased considerably. They had returned to normal by 22 min after injection. The plasma concentrations of AR-L 57 CL of 2 - 5 mug-equivalents/ml showed a highly significant correlation with the decrease in systolic time intervals. Both systolic and diastolic blood pressure rose briefly after injection. The AV conduction time fell initially and the heart-rate increased briefly. Thus AR-L 57 CL was shown to be a short acting drug with a high degree of positive inotropic action. It did not cause bradycardia or increase atrioventricular transmission time and appeared to be easily controllable.", "PMID": 976305}
{"id": "pubmed23n0032_10656", "title": "Influence of sex on the clinical pharmacology of flutiorex, a new anorectic drug.", "content": "The effects of flutiorex, a new anorectic agent, and of a placebo on food intake and the activity of the sympathetic and central nervous systems have been compared in a double blind trial in two groups of healthy volunteers, one of five males and the other of five females. Flutiorex 20 mg orally had a significant anorectic effect both in males and females, the observed reduction in food intake being 34.0 and 27.5%, respectively. It caused alpha-adrenergic stimulation (blood pressure and pupil diameter) and central nervous system excitation (critical flicker frequency), both of which were more marked in males than in females. Flutiorex was better tolerated by women than by men. Measurement of the blood level of flutiorex and its de-ethylated metabolite, norflutiorex, showed that both compounds were detectable in four of the five male subjects, but in only one of the five females. Sex-linked differences in the pharmacokinetics of flutiorex may explain the greater intensity of its effects in males.", "contents": "Influence of sex on the clinical pharmacology of flutiorex, a new anorectic drug. The effects of flutiorex, a new anorectic agent, and of a placebo on food intake and the activity of the sympathetic and central nervous systems have been compared in a double blind trial in two groups of healthy volunteers, one of five males and the other of five females. Flutiorex 20 mg orally had a significant anorectic effect both in males and females, the observed reduction in food intake being 34.0 and 27.5%, respectively. It caused alpha-adrenergic stimulation (blood pressure and pupil diameter) and central nervous system excitation (critical flicker frequency), both of which were more marked in males than in females. Flutiorex was better tolerated by women than by men. Measurement of the blood level of flutiorex and its de-ethylated metabolite, norflutiorex, showed that both compounds were detectable in four of the five male subjects, but in only one of the five females. Sex-linked differences in the pharmacokinetics of flutiorex may explain the greater intensity of its effects in males.", "PMID": 976306}
{"id": "pubmed23n0032_10657", "title": "Pharmacokinetics of amitriptyline infused intravenously in man.", "content": "Amitriptyline was given to four male volunteers by constant rate intravenous infusion. Blood samples were collected before, during and at various times after the infusion for estimation of the serum concentrations of amitriptyline. The level of nortriptyline never reached a detectable level. A two compartment open model was shown to be applicable to the data obtained. The meaning of the parameters obtained by a non-linear, least squares curve fitting procedure is discussed and the values are compared to those recently published for nortriptyline. The calculated biological half-life of amitriptyline was about 17 hours, a figure which differs considerably from previously calculated values for volunteers, but is in accordance with some newer results from patients.", "contents": "Pharmacokinetics of amitriptyline infused intravenously in man. Amitriptyline was given to four male volunteers by constant rate intravenous infusion. Blood samples were collected before, during and at various times after the infusion for estimation of the serum concentrations of amitriptyline. The level of nortriptyline never reached a detectable level. A two compartment open model was shown to be applicable to the data obtained. The meaning of the parameters obtained by a non-linear, least squares curve fitting procedure is discussed and the values are compared to those recently published for nortriptyline. The calculated biological half-life of amitriptyline was about 17 hours, a figure which differs considerably from previously calculated values for volunteers, but is in accordance with some newer results from patients.", "PMID": 976307}
{"id": "pubmed23n0032_10658", "title": "Quantitative contributions of IgG, IgM and C3 to erythrophagocytosis and rosette formation by peritoneal macrophages, and anti-opsonin activity of dextran sulfate 500.", "content": "In vitro phagocytosis by guinea pig peritoneal macrophages of immune complexes (EA) was shown to be dependent on IgG antibody in a dose-dependent fashion. C3b enhanced phagocytosis of EA at limited IgG antibody concentrations only. When IgM antibody was used for sensitization of sheep red blood cells (SRBC), phagocytosis and rosette formation did not occur in the absence of bound C3. The polyanion, dextran sulfate 500 (DS), was shown to depress both rosette formation and phagocytosis of EAIgG, C1423 and EAIgMC1423, as well as immune adherence of human group 0  erythrocytes and hemolytic activity of C3. This effect of DS was seen only when it was actually present in the incubation medium.", "contents": "Quantitative contributions of IgG, IgM and C3 to erythrophagocytosis and rosette formation by peritoneal macrophages, and anti-opsonin activity of dextran sulfate 500. In vitro phagocytosis by guinea pig peritoneal macrophages of immune complexes (EA) was shown to be dependent on IgG antibody in a dose-dependent fashion. C3b enhanced phagocytosis of EA at limited IgG antibody concentrations only. When IgM antibody was used for sensitization of sheep red blood cells (SRBC), phagocytosis and rosette formation did not occur in the absence of bound C3. The polyanion, dextran sulfate 500 (DS), was shown to depress both rosette formation and phagocytosis of EAIgG, C1423 and EAIgMC1423, as well as immune adherence of human group 0  erythrocytes and hemolytic activity of C3. This effect of DS was seen only when it was actually present in the incubation medium.", "PMID": 976308}
{"id": "pubmed23n0032_10659", "title": "Analysis of antibody populations to oligo-D-alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum.", "content": "Allotypically marked antisera to the hapten oligo-D-alanine were studied over periods up to 10 months in individual mice and in serial transfers of both nonimmune and memory cells to irradiated recipients. Parameters observed were divalent association rate constants, affinity and autoradiography of isoelectric focusing (IEF) spectra. The two allotypic subpopulations of heterozygous mice are shown to be independent with respect to affinity. Association rate constant, affinity, and IEF spectra of the antibody populations remain largely stable in most mice over prolonged periods. There is an occasional isolated dramatic change in a single property of one allotypic subpopulation; this may be in the direction of either lower or higher rate constant or affinity. While different recipients of naive spleen cells from a single donor, when immunized, differ one from the other in the measured antibody properties, the characteristics now established in each recipient persist in a lineage of transferred memory cells up to 4 generations, with infrequent changes in isolated properties of only one allotypic subpopulation. The limited changes observed would be in agreement with a model of moderate somatic variability superimposed on a small spectrum of germ line genes.", "contents": "Analysis of antibody populations to oligo-D-alanine. VIII. Sequential studies on properties of antibody subpopulations: rate constant, affinity, and isoelectric focusing spectrum. Allotypically marked antisera to the hapten oligo-D-alanine were studied over periods up to 10 months in individual mice and in serial transfers of both nonimmune and memory cells to irradiated recipients. Parameters observed were divalent association rate constants, affinity and autoradiography of isoelectric focusing (IEF) spectra. The two allotypic subpopulations of heterozygous mice are shown to be independent with respect to affinity. Association rate constant, affinity, and IEF spectra of the antibody populations remain largely stable in most mice over prolonged periods. There is an occasional isolated dramatic change in a single property of one allotypic subpopulation; this may be in the direction of either lower or higher rate constant or affinity. While different recipients of naive spleen cells from a single donor, when immunized, differ one from the other in the measured antibody properties, the characteristics now established in each recipient persist in a lineage of transferred memory cells up to 4 generations, with infrequent changes in isolated properties of only one allotypic subpopulation. The limited changes observed would be in agreement with a model of moderate somatic variability superimposed on a small spectrum of germ line genes.", "PMID": 976309}
{"id": "pubmed23n0032_10660", "title": "Enzymatic modification of lymphocyte receptors for antigen. III. Resistance of receptors to trypsin at the peak of the immune response.", "content": "Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunization, 80-90% were trypsin-resistant, indicating an increase of about 50-fold in trypsin-resistant antigen-binding cells per spleen. In contrast, trypsin-sensitive cells per spleen were only 4-fold higher on day 5 than before immunization. The rise in % trypsin sensitivity preceded the increase in rosettes per spleen, implying that immunization produced a preferential increase in trypsin-resistant antigen binding cells partly by converting sensitive cells to resistant cells. After the 5th day, the trypsin sensitivity of antigen-binding cells slowly returned toward the unimmunized level, but a booster injection of SRBC restored trypsin resistance. Trypsin resistance was not lost in the presence of sodium azide or protein synthesis inhibitors. But a slightly increased trypsin susceptibility was conferred by 2-deoxyglucose, implying that glycolysis or the glycosylation of protein may be involved in maintaining trypsin resistance.", "contents": "Enzymatic modification of lymphocyte receptors for antigen. III. Resistance of receptors to trypsin at the peak of the immune response. Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunization, 80-90% were trypsin-resistant, indicating an increase of about 50-fold in trypsin-resistant antigen-binding cells per spleen. In contrast, trypsin-sensitive cells per spleen were only 4-fold higher on day 5 than before immunization. The rise in % trypsin sensitivity preceded the increase in rosettes per spleen, implying that immunization produced a preferential increase in trypsin-resistant antigen binding cells partly by converting sensitive cells to resistant cells. After the 5th day, the trypsin sensitivity of antigen-binding cells slowly returned toward the unimmunized level, but a booster injection of SRBC restored trypsin resistance. Trypsin resistance was not lost in the presence of sodium azide or protein synthesis inhibitors. But a slightly increased trypsin susceptibility was conferred by 2-deoxyglucose, implying that glycolysis or the glycosylation of protein may be involved in maintaining trypsin resistance.", "PMID": 976310}
{"id": "pubmed23n0032_10661", "title": "Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. II. The mouse nonadherent K cell.", "content": "A cell of lymphoid morphology capable of killing antibody-coated chicken erythrocytes was isolated from nonimmune mouse spleen using a combination of carbonyl iron treatment and glass bead column passage. This non-phagocytic effector cell, which is referred to as the nonadherent K (killer) cell, is distinguished from the non-phagocytic myeloid K cell described earlier (Greenberg, A.H., Shen, L. and Roitt, I.M., Clin. Exp. Immunol. 1973. 15:251) by its relatively weak surface adherence properties and low concentration within the mouse spleen. The cell is further characterized by its relatively large size, lack of theta or immunoglobulin determinants, the presence of Mg++-independent complement receptors, affinity for aggregated IgG2 myeloma proteins, inhibition by cytochalasin B and good survival in cell culture. The possible lineage of the cell is discussed.", "contents": "Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. II. The mouse nonadherent K cell. A cell of lymphoid morphology capable of killing antibody-coated chicken erythrocytes was isolated from nonimmune mouse spleen using a combination of carbonyl iron treatment and glass bead column passage. This non-phagocytic effector cell, which is referred to as the nonadherent K (killer) cell, is distinguished from the non-phagocytic myeloid K cell described earlier (Greenberg, A.H., Shen, L. and Roitt, I.M., Clin. Exp. Immunol. 1973. 15:251) by its relatively weak surface adherence properties and low concentration within the mouse spleen. The cell is further characterized by its relatively large size, lack of theta or immunoglobulin determinants, the presence of Mg++-independent complement receptors, affinity for aggregated IgG2 myeloma proteins, inhibition by cytochalasin B and good survival in cell culture. The possible lineage of the cell is discussed.", "PMID": 976311}
{"id": "pubmed23n0032_10662", "title": "Close linkage between genes coding for allotypic markers on chicken IgG and IgM.", "content": "The allotypic markers Gla and Gld (present on chicken IgG molecules) and Mla and Mlc (present on IgM) have previously been defined. The inheritance of these markers was studied in the F2 progeny of a cross between birds of the inbred lines CB (Gla+,d-,Mla+,c-) and WA (Gla-,d+,Mla-,c+). The results suggest that antigens Gla and Gld, and Mla and Mlc, are genetically controlled by alleles at two loci (Gl and Ml, respectively) and also show that these loci are closely linked.", "contents": "Close linkage between genes coding for allotypic markers on chicken IgG and IgM. The allotypic markers Gla and Gld (present on chicken IgG molecules) and Mla and Mlc (present on IgM) have previously been defined. The inheritance of these markers was studied in the F2 progeny of a cross between birds of the inbred lines CB (Gla+,d-,Mla+,c-) and WA (Gla-,d+,Mla-,c+). The results suggest that antigens Gla and Gld, and Mla and Mlc, are genetically controlled by alleles at two loci (Gl and Ml, respectively) and also show that these loci are closely linked.", "PMID": 976312}
{"id": "pubmed23n0032_10663", "title": "Precipitin and mitogenic behavior of dimeric and tetrameric concanavalin A.", "content": "The results of double diffusion and quantitative precipitin tests show that in contrast to the concanavalin A tetramer, the dimer is incapable of precipitating glycogen. The results support previous work suggesting that dimers composed of two fragmented monomer units possess only one binding site for carbohydrate (McKenzie and Sawyer, J. Biol. Chem. 1973. 248: 549). On the other hand, dimer and tetramer species had similar mitogenic activity as measured by the incorporation of radioactive thymidine by mouse spleen lymphocytes and induced cap formation to similar degrees. Results are discussed in terms of the affinity and valency of the polymeric forms and their stability at the cell surface.", "contents": "Precipitin and mitogenic behavior of dimeric and tetrameric concanavalin A. The results of double diffusion and quantitative precipitin tests show that in contrast to the concanavalin A tetramer, the dimer is incapable of precipitating glycogen. The results support previous work suggesting that dimers composed of two fragmented monomer units possess only one binding site for carbohydrate (McKenzie and Sawyer, J. Biol. Chem. 1973. 248: 549). On the other hand, dimer and tetramer species had similar mitogenic activity as measured by the incorporation of radioactive thymidine by mouse spleen lymphocytes and induced cap formation to similar degrees. Results are discussed in terms of the affinity and valency of the polymeric forms and their stability at the cell surface.", "PMID": 976313}
{"id": "pubmed23n0032_10664", "title": "Genetic control of the immune response to the radom linear terpolymer of L-glutamic acid, L-lysine and L-leucine (GLleu) by complementing Ir genes.", "content": "Three levels of humoral responsiveness (none, high or marginal) were noted following secondary immunization with random linear terpolymer of L-glutamic acid, L-lysine and L-leucine (GLleu). These immune responses were under dual H-2-linked Ir gene control. Gene complementation was observed in appropriate F1 offspring between two nonresponder strains and in selected intra-I region recombinants derived from crossing over between nonresponder parental haplotypes. One of the Ir-GLleu genes, beta, was localized within the I-A or I-B subregions. The other Ir-GLleu locus, termed alpha, was mapped in the I-C or S subregions. Differences in the strain distribution of immune responsiveness to GLleu and related terpolymer GLphe (which is also under complementing Ir gene control) are primarily attributable to differences among the Ir-GLleu and Ir-GLphe beta alleles. A correlation exists between the presence of the Ir-GLleu alpha(\u00bfallele and the gene coding for specificity Ia.7.", "contents": "Genetic control of the immune response to the radom linear terpolymer of L-glutamic acid, L-lysine and L-leucine (GLleu) by complementing Ir genes. Three levels of humoral responsiveness (none, high or marginal) were noted following secondary immunization with random linear terpolymer of L-glutamic acid, L-lysine and L-leucine (GLleu). These immune responses were under dual H-2-linked Ir gene control. Gene complementation was observed in appropriate F1 offspring between two nonresponder strains and in selected intra-I region recombinants derived from crossing over between nonresponder parental haplotypes. One of the Ir-GLleu genes, beta, was localized within the I-A or I-B subregions. The other Ir-GLleu locus, termed alpha, was mapped in the I-C or S subregions. Differences in the strain distribution of immune responsiveness to GLleu and related terpolymer GLphe (which is also under complementing Ir gene control) are primarily attributable to differences among the Ir-GLleu and Ir-GLphe beta alleles. A correlation exists between the presence of the Ir-GLleu alpha(\u00bfallele and the gene coding for specificity Ia.7.", "PMID": 976314}
{"id": "pubmed23n0032_10665", "title": "Analysis of cardiac chronotropic responses to some autonomic blocking agents in conscious trained dogs.", "content": "The changes of heart rate in response to i.v. administration of methylatropine (0.5 mg/kg) and/or propranolol (2 mg/Kg) or practolol (2.5 mg/Kg) were studied in conscious trained dogs. Cholinergic blockade alone or combined blockade of sympathetic and parasympathetic effector systems resulted in cardiac acceleration. Conversely, beta-adrenoceptor antagonism with either propranolol or practolol reduced heart rate. The data were analysed by means of a new method, whereby the heart (HRN) of the dog is considered to be the product of the intrinsic heart rate (HR0) and 3 further factors: HRN-HR0 times S times V times W (Multiplicative model). 2 of these factors represent the tonic sympathetic (S) and parasympathetic (V) influences, whereas the third (W) represents the sympathetic-parasympathetic interaction. This type of analysis reveals that W was approximately 1, i.e., the sympathetic-parasympathetic interaction did not play any significant role in determining the heart rate of conscious resting dogs (HRN = HRO-S-V-W = HRO-S-V). The change of heart rate due to the action of parasympathetic system (-53% of the intrinsic heart rate) was more important than the change caused by the action of the sympathetic system (26% of the intrinsic heart rate).", "contents": "Analysis of cardiac chronotropic responses to some autonomic blocking agents in conscious trained dogs. The changes of heart rate in response to i.v. administration of methylatropine (0.5 mg/kg) and/or propranolol (2 mg/Kg) or practolol (2.5 mg/Kg) were studied in conscious trained dogs. Cholinergic blockade alone or combined blockade of sympathetic and parasympathetic effector systems resulted in cardiac acceleration. Conversely, beta-adrenoceptor antagonism with either propranolol or practolol reduced heart rate. The data were analysed by means of a new method, whereby the heart (HRN) of the dog is considered to be the product of the intrinsic heart rate (HR0) and 3 further factors: HRN-HR0 times S times V times W (Multiplicative model). 2 of these factors represent the tonic sympathetic (S) and parasympathetic (V) influences, whereas the third (W) represents the sympathetic-parasympathetic interaction. This type of analysis reveals that W was approximately 1, i.e., the sympathetic-parasympathetic interaction did not play any significant role in determining the heart rate of conscious resting dogs (HRN = HRO-S-V-W = HRO-S-V). The change of heart rate due to the action of parasympathetic system (-53% of the intrinsic heart rate) was more important than the change caused by the action of the sympathetic system (26% of the intrinsic heart rate).", "PMID": 976316}
{"id": "pubmed23n0032_10666", "title": "The roles of noradrenaline and dopamine in contraversive circling behaviour seen after unilateral electrolytic lesions of the locus coeruleus.", "content": "Unilateral electrolytic lesions of the locus coeruleus in rats result in spontaneous ipsiversive rotation, which is then replaced by contraversive rotation. One week after lesioning, when spontaneous turning ceases, apomorphine and d-amphetamine elicit contraversive circling behaviour, which was not affected by noradrenergic receptor blockade but was abolished by dopamine receptor blockade. The drug-induced contraversive circling response was also reproduced by piribedil but not clonidine. Combined unilateral electrolytic locus coeruleus and substantia nigra lesions on the same side resulted in apomorphine- and d-amphetamine-induced ipsilateral rotational behaviour which was indistinguishable from that seen with substantia nigra lesions alone. In rats with unilateral locus coeruleus lesions, the dose of intrastriatally injected apomorphine required to produce circling was less on the lesioned than the non-lesioned side. Direct injection of noradrenaline into one substantia nigra caused contraversive circling. Direct injection of phenoxybenzamine into one substantia nigra followed by apomorphine caused ipsiversive circling. The results suggest that the circling behaviour seen after unilateral locus coeruleus lesions depends on an asymmetry of striatal dopamine receptor activity and are consistent with a proposed coeruleus-nigral noradrenergic pathway, which enhances impulse flow in the dopaminergic nigrostriatal system.", "contents": "The roles of noradrenaline and dopamine in contraversive circling behaviour seen after unilateral electrolytic lesions of the locus coeruleus. Unilateral electrolytic lesions of the locus coeruleus in rats result in spontaneous ipsiversive rotation, which is then replaced by contraversive rotation. One week after lesioning, when spontaneous turning ceases, apomorphine and d-amphetamine elicit contraversive circling behaviour, which was not affected by noradrenergic receptor blockade but was abolished by dopamine receptor blockade. The drug-induced contraversive circling response was also reproduced by piribedil but not clonidine. Combined unilateral electrolytic locus coeruleus and substantia nigra lesions on the same side resulted in apomorphine- and d-amphetamine-induced ipsilateral rotational behaviour which was indistinguishable from that seen with substantia nigra lesions alone. In rats with unilateral locus coeruleus lesions, the dose of intrastriatally injected apomorphine required to produce circling was less on the lesioned than the non-lesioned side. Direct injection of noradrenaline into one substantia nigra caused contraversive circling. Direct injection of phenoxybenzamine into one substantia nigra followed by apomorphine caused ipsiversive circling. The results suggest that the circling behaviour seen after unilateral locus coeruleus lesions depends on an asymmetry of striatal dopamine receptor activity and are consistent with a proposed coeruleus-nigral noradrenergic pathway, which enhances impulse flow in the dopaminergic nigrostriatal system.", "PMID": 976315}
{"id": "pubmed23n0032_10667", "title": "Effects of physostigmine and electrical stimulation on the acetylcholine content of the guinea-pig ileum.", "content": "Incubation with physostigmine (7.7 muM) caused an approximately 2 fold increase in the acetylcholine content of the myenteric plexus--longitudinal muscle preparation of the guinea-pig ileum. This effect was due mainly to an increase in 'free' acetylcholine, which was directly assayable in either the homogenate after removal of cell debris or the supernatant fraction (100,000 g for 60 min) after subcellular fractionation. Acetylcholine output during stimulation at 0.017, 0.1 or 1 Hz was maintained for 60 min at a rate 2--4 times greater than the non-stimulated output; there was no change in content. At 10 HZ, output was high at the start of stimulation and then decreased continuously; there was a proportionate loss of mainly 'free' acetylcholine from the tissue. Mn2+, hexamethonium, morphine and noradrenaline, which depressed acetylcholine output during stimulation at 0.1 HZ, had no effect on the acetylcholine content nor did they affect the increase in acetylcholine content during incubation with physostigmine.", "contents": "Effects of physostigmine and electrical stimulation on the acetylcholine content of the guinea-pig ileum. Incubation with physostigmine (7.7 muM) caused an approximately 2 fold increase in the acetylcholine content of the myenteric plexus--longitudinal muscle preparation of the guinea-pig ileum. This effect was due mainly to an increase in 'free' acetylcholine, which was directly assayable in either the homogenate after removal of cell debris or the supernatant fraction (100,000 g for 60 min) after subcellular fractionation. Acetylcholine output during stimulation at 0.017, 0.1 or 1 Hz was maintained for 60 min at a rate 2--4 times greater than the non-stimulated output; there was no change in content. At 10 HZ, output was high at the start of stimulation and then decreased continuously; there was a proportionate loss of mainly 'free' acetylcholine from the tissue. Mn2+, hexamethonium, morphine and noradrenaline, which depressed acetylcholine output during stimulation at 0.1 HZ, had no effect on the acetylcholine content nor did they affect the increase in acetylcholine content during incubation with physostigmine.", "PMID": 976317}
{"id": "pubmed23n0032_10668", "title": "Effects of salsolinol on smooth muscle responses to various biogenic amines.", "content": "Isolated smooth muscle tissues from rats, guinea pigs and rabbits were used to investigate pharmacological effects of salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline). By itself, salsolinol did not produce any profound or sustained sympathomimetic or 5-hydroxytryptamine-like activity in the concentrations used in these experiments (7.7 and 77  muM). Salsolinol was found to antagonize the contraction of the rabbit aorta induced by noradrenaline but did not affect the adrenaline-induced relaxation of the guinea pig tracheal chain. On both the rat fundus and oestrus rat uterus, salsolinol exhibited significant anti-5-hydroxytryptamine activity but responses of these tissues to acetylcholine and histamine remained unaffected, indicating that the action was not non-specific. These results suggest that in vivo formation of salsolinol after ethanol ingestion could produce significant pharmacological effects.", "contents": "Effects of salsolinol on smooth muscle responses to various biogenic amines. Isolated smooth muscle tissues from rats, guinea pigs and rabbits were used to investigate pharmacological effects of salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline). By itself, salsolinol did not produce any profound or sustained sympathomimetic or 5-hydroxytryptamine-like activity in the concentrations used in these experiments (7.7 and 77  muM). Salsolinol was found to antagonize the contraction of the rabbit aorta induced by noradrenaline but did not affect the adrenaline-induced relaxation of the guinea pig tracheal chain. On both the rat fundus and oestrus rat uterus, salsolinol exhibited significant anti-5-hydroxytryptamine activity but responses of these tissues to acetylcholine and histamine remained unaffected, indicating that the action was not non-specific. These results suggest that in vivo formation of salsolinol after ethanol ingestion could produce significant pharmacological effects.", "PMID": 976318}
{"id": "pubmed23n0032_10669", "title": "Absence of a 'cholinergic link' in the apomorphine-induced feedback inhibition of dopamine synthesis in rat striatum.", "content": "The blocking effect of apomorphine on the rise in striatal dopamine (DA) content, induced by 1-hydroxy-3-amino-pyrrolidone-2 (HA-966) was taken as a measure for the intrastriatal feedback inhibition of DA synthesis. The effects of cholinergic drugs on this feedback system were assessed in order to verify the hypothesis that this mechanism is mediated via an intrastriatal cholinergic link. We presumed that DA receptors were located on a cholinergic neuron, while the cholinergic terminals in turn made direct or indirect axon-axonal contact with the dopaminergic nigro-striatal pathway (N.S.P.). Although cholinergic agents could modify the effect of HA-966 on striatal DA content, it proved to be impossible to counteract the blocking effect of apomorphine with cholinergic drugs as was to be expected. Therefore we concluded that the effect of apomorphine was not brought about in the way which had been postulated.", "contents": "Absence of a 'cholinergic link' in the apomorphine-induced feedback inhibition of dopamine synthesis in rat striatum. The blocking effect of apomorphine on the rise in striatal dopamine (DA) content, induced by 1-hydroxy-3-amino-pyrrolidone-2 (HA-966) was taken as a measure for the intrastriatal feedback inhibition of DA synthesis. The effects of cholinergic drugs on this feedback system were assessed in order to verify the hypothesis that this mechanism is mediated via an intrastriatal cholinergic link. We presumed that DA receptors were located on a cholinergic neuron, while the cholinergic terminals in turn made direct or indirect axon-axonal contact with the dopaminergic nigro-striatal pathway (N.S.P.). Although cholinergic agents could modify the effect of HA-966 on striatal DA content, it proved to be impossible to counteract the blocking effect of apomorphine with cholinergic drugs as was to be expected. Therefore we concluded that the effect of apomorphine was not brought about in the way which had been postulated.", "PMID": 976319}
{"id": "pubmed23n0032_10670", "title": "A comparison of the vasodepressor effects of the cyclic effects of the cyclic endoperoxides PGG, and PGH2 with those of PGD2 and PGE2 in hypertensive and normotensive rats.", "content": "The vasodepressor actions of the cyclic endoperoxides PGG2 and PGH2 were compared with those of their products PGD2 and PGE2 using anaesthetised normotensive and genetically hypertensive rats. Given into the aortic arch of normotensives PGE2 was approximately 6 times more potent than PGH2  and 11 times more potent than PGG2 and PGD2. Hypertensive animals were 1.5-10 times more sensitive than normotensives to the depressor effects of PGG2 and PGH2, but their sensitivity to either PGD2 or PGE2 was similar. Thus in hypertensives the endoperoxides may be converted more readily to PGE2 and other products. In both types of rat PGG2 and PGH2 given intravenously were as active or more active than after intra-arterial. Therefore PGG2 and PGH2 may be converted more readily to more active products during passage through the lungs but whereas small doses of PGE2 are almost completely eliminated large doses may saturate uplmonary removal mechanisms.", "contents": "A comparison of the vasodepressor effects of the cyclic effects of the cyclic endoperoxides PGG, and PGH2 with those of PGD2 and PGE2 in hypertensive and normotensive rats. The vasodepressor actions of the cyclic endoperoxides PGG2 and PGH2 were compared with those of their products PGD2 and PGE2 using anaesthetised normotensive and genetically hypertensive rats. Given into the aortic arch of normotensives PGE2 was approximately 6 times more potent than PGH2  and 11 times more potent than PGG2 and PGD2. Hypertensive animals were 1.5-10 times more sensitive than normotensives to the depressor effects of PGG2 and PGH2, but their sensitivity to either PGD2 or PGE2 was similar. Thus in hypertensives the endoperoxides may be converted more readily to PGE2 and other products. In both types of rat PGG2 and PGH2 given intravenously were as active or more active than after intra-arterial. Therefore PGG2 and PGH2 may be converted more readily to more active products during passage through the lungs but whereas small doses of PGE2 are almost completely eliminated large doses may saturate uplmonary removal mechanisms.", "PMID": 976320}
{"id": "pubmed23n0032_10671", "title": "Effects of prostaglandin E1 on the positive inotropic actions of noradrenaline, nerve stimulation and calcium in the isolated blood-perfused papillary muscle of the dog.", "content": "The effects of PGE1 on the dog heart were studied using the blood-perfused sinus node and papillary muscle preparations isolated separately from the same animal. PGE1 administered into the papillary muscle artery as bolus injections in doses of 1-1000 ng caused a dose-dependent increase of the developed tension and dT/dt of the papillary muscle. The effect was not inhibited by the beta-adrenoceptor blocking agent pindolol. PGE1 injected into the sinus node artery in doses of 3-300 ng did not change the rate of contraction of the sinus node preparation. PGE1 in the blood concentrations of 4.4 X 10(-9) to 1.7 X 10(-7) M enhanced the positive inotropic responses to noradrenalin and field stimulation as well as to calcium. The influence of PGE1 on the positive inotropic effect of perivascular nerve stimulation was not consistent: the action of perivascular nerve stimulation was enhanced by PGE1 in the majority of preparations but was reduced in one third of preparations. PGE1 in the same blood concentrations as used in the papillary muscle significantly depressed the positive chronotropic responses to noradrenaline and dopamine. The present results indicate that PGE1 induces multiple actions on the dog heart. Its predominant effect on the ventricular myocardium appears to be enhancement of the adrenergic stimuli probably via the facilitation of calcium movement through the myocardial cell membrane. In addition, PGE1 may decrease the sensitivity of beta-adrenoceptors to adrenergic stimuli in the sinus node.", "contents": "Effects of prostaglandin E1 on the positive inotropic actions of noradrenaline, nerve stimulation and calcium in the isolated blood-perfused papillary muscle of the dog. The effects of PGE1 on the dog heart were studied using the blood-perfused sinus node and papillary muscle preparations isolated separately from the same animal. PGE1 administered into the papillary muscle artery as bolus injections in doses of 1-1000 ng caused a dose-dependent increase of the developed tension and dT/dt of the papillary muscle. The effect was not inhibited by the beta-adrenoceptor blocking agent pindolol. PGE1 injected into the sinus node artery in doses of 3-300 ng did not change the rate of contraction of the sinus node preparation. PGE1 in the blood concentrations of 4.4 X 10(-9) to 1.7 X 10(-7) M enhanced the positive inotropic responses to noradrenalin and field stimulation as well as to calcium. The influence of PGE1 on the positive inotropic effect of perivascular nerve stimulation was not consistent: the action of perivascular nerve stimulation was enhanced by PGE1 in the majority of preparations but was reduced in one third of preparations. PGE1 in the same blood concentrations as used in the papillary muscle significantly depressed the positive chronotropic responses to noradrenaline and dopamine. The present results indicate that PGE1 induces multiple actions on the dog heart. Its predominant effect on the ventricular myocardium appears to be enhancement of the adrenergic stimuli probably via the facilitation of calcium movement through the myocardial cell membrane. In addition, PGE1 may decrease the sensitivity of beta-adrenoceptors to adrenergic stimuli in the sinus node.", "PMID": 976321}
{"id": "pubmed23n0032_10672", "title": "Effect of neuroleptics and of combinations of d-amphetamine and neuroleptics on 3H-dopamine uptake by homogenates from rat striatum.", "content": "Triperidol was found to be a more potent 3H-dopamine uptake inhibitor than chlorpromazine in homogenates from rat striatum. Inhibition kinetics were competitive for triperidol and non-competitive for chlorpromazine. When drugs were given in vivo, d-amphetamine (10 mg/kg) blocked the 3H-dopamine uptake by about 50% whereas the neuroleptics did not modify the process even at highly sedating doses. Combined treatments with d-amphetamine and neuroleptics showed that only triperidol potentiated the blocking effect of d-amphetamine on 3-H-dopamine uptake. However, such a potentiation was not observed when triperidol and d-amphetamine were simultaneously added in vitro. The results tend to suggest that the postulated actions of neuroleptics on presynaptic sites in the striatum may be more important with the butyrophenone, triperidol than with the phenothiazine, chlorpromazine.", "contents": "Effect of neuroleptics and of combinations of d-amphetamine and neuroleptics on 3H-dopamine uptake by homogenates from rat striatum. Triperidol was found to be a more potent 3H-dopamine uptake inhibitor than chlorpromazine in homogenates from rat striatum. Inhibition kinetics were competitive for triperidol and non-competitive for chlorpromazine. When drugs were given in vivo, d-amphetamine (10 mg/kg) blocked the 3H-dopamine uptake by about 50% whereas the neuroleptics did not modify the process even at highly sedating doses. Combined treatments with d-amphetamine and neuroleptics showed that only triperidol potentiated the blocking effect of d-amphetamine on 3-H-dopamine uptake. However, such a potentiation was not observed when triperidol and d-amphetamine were simultaneously added in vitro. The results tend to suggest that the postulated actions of neuroleptics on presynaptic sites in the striatum may be more important with the butyrophenone, triperidol than with the phenothiazine, chlorpromazine.", "PMID": 976322}
{"id": "pubmed23n0032_10673", "title": "The effects of isoxsuprine on non-myelinated nerve fibres.", "content": "A study has been made of the effect of isoxsuprine on non-myelinated nerve fibres of the desheathed rabbit vagus. Isoxsuprine did not affect the membrane potential, the membrane resistance, or the sodium pump activity. Isoxsuprine diminished the amplitude and rise time of the compound action potential. This indicates that isoxsuprine decreased the peak sodium current during the action potential. This local anesthetic action of isoxsupring is more pronounced than that of lidocaine.", "contents": "The effects of isoxsuprine on non-myelinated nerve fibres. A study has been made of the effect of isoxsuprine on non-myelinated nerve fibres of the desheathed rabbit vagus. Isoxsuprine did not affect the membrane potential, the membrane resistance, or the sodium pump activity. Isoxsuprine diminished the amplitude and rise time of the compound action potential. This indicates that isoxsuprine decreased the peak sodium current during the action potential. This local anesthetic action of isoxsupring is more pronounced than that of lidocaine.", "PMID": 976323}
{"id": "pubmed23n0032_10674", "title": "Effects of verapamil on regional myocardial blood flow and ST segment. Role of the induced bradycardia.", "content": "The effects of verapamil on regional myocardial blood flow and on the ST segment were studied in both normal and ischemic regions in dogs with and without cardiac pacing. In the absence of cardiac pacing, verapamil (0.05 mg/kg/min/10 min) induced marked bradycardia, a drop in blood pressure and an increase in epicardial and endocardial flows both in normal and ischemic regions of the heart. However, in ischemic regions, redistribution was favorable since the endo/epi ratio increased from 0.46 to 0.61 (p less than 0.01) whereas this ratio did not vary in normal regions (0.94 vs. 0.92). This increase is accompanied by less marked ST segment elevation during coronary occlusion in dogs treated with verapamil. In dogs with cardiac pacing, administration of verapamil under the same conditions again induced a drop in blood pressure and, in normal regions only, a homogeneous but less marked increase in epicardial and endocardial flows, the endo/epi ratio varying from 0.94 to 0.98. In contrast, in ischemic regions, blood flow was not modified by verapamil, but the endo/epi ratio dropped from 0.46 to 0.31 (p less than 0.01), while the ST segment elevation observed did not differ from that recorded during the control occlusion. These results demonstrate the major role of verapamil-induced bradycardia in the anti-anginal affects of this drug.", "contents": "Effects of verapamil on regional myocardial blood flow and ST segment. Role of the induced bradycardia. The effects of verapamil on regional myocardial blood flow and on the ST segment were studied in both normal and ischemic regions in dogs with and without cardiac pacing. In the absence of cardiac pacing, verapamil (0.05 mg/kg/min/10 min) induced marked bradycardia, a drop in blood pressure and an increase in epicardial and endocardial flows both in normal and ischemic regions of the heart. However, in ischemic regions, redistribution was favorable since the endo/epi ratio increased from 0.46 to 0.61 (p less than 0.01) whereas this ratio did not vary in normal regions (0.94 vs. 0.92). This increase is accompanied by less marked ST segment elevation during coronary occlusion in dogs treated with verapamil. In dogs with cardiac pacing, administration of verapamil under the same conditions again induced a drop in blood pressure and, in normal regions only, a homogeneous but less marked increase in epicardial and endocardial flows, the endo/epi ratio varying from 0.94 to 0.98. In contrast, in ischemic regions, blood flow was not modified by verapamil, but the endo/epi ratio dropped from 0.46 to 0.31 (p less than 0.01), while the ST segment elevation observed did not differ from that recorded during the control occlusion. These results demonstrate the major role of verapamil-induced bradycardia in the anti-anginal affects of this drug.", "PMID": 976324}
{"id": "pubmed23n0032_10675", "title": "Blockade of the specific lethal effects of narcotic analgesics in the mouse.", "content": "The capacity of the narcotic antagonists naloxone and nalorphine and the benzodiazepine derivatives diazepam and oxazepam to increase the LD50s of the narcotic analgesics morphine and methadone administered at convulsant doses was eveluated in the mouse. Naloxone produced a dose-related increase in the LD50s of both morphine and methadone. Iiazepam and oxazepam were also effective in increasing the LD50s of the narcotics; this effect was additive with that of naloxone. However, the maximal increase in the LD50s of the narcotics produced by pretreatment with naloxone alone was not increased further by the combined pretreatment of naloxone and a benzodiazepine. The anticonvulsant trimethadione did not elevate the LD50s of methadone, nor did it potentiate the effects of naloxone. These results suggest that the benzodiazepines may reduce the lethality of narcotic analgesics administered at high doses by a mechanism other than by an anticonvulsant effect alone. Therefore, the present results support the conclusion that the capacity to increase the convulsant LD50 of the narcotic analgesics is a general property of the narcotic antagonists.", "contents": "Blockade of the specific lethal effects of narcotic analgesics in the mouse. The capacity of the narcotic antagonists naloxone and nalorphine and the benzodiazepine derivatives diazepam and oxazepam to increase the LD50s of the narcotic analgesics morphine and methadone administered at convulsant doses was eveluated in the mouse. Naloxone produced a dose-related increase in the LD50s of both morphine and methadone. Iiazepam and oxazepam were also effective in increasing the LD50s of the narcotics; this effect was additive with that of naloxone. However, the maximal increase in the LD50s of the narcotics produced by pretreatment with naloxone alone was not increased further by the combined pretreatment of naloxone and a benzodiazepine. The anticonvulsant trimethadione did not elevate the LD50s of methadone, nor did it potentiate the effects of naloxone. These results suggest that the benzodiazepines may reduce the lethality of narcotic analgesics administered at high doses by a mechanism other than by an anticonvulsant effect alone. Therefore, the present results support the conclusion that the capacity to increase the convulsant LD50 of the narcotic analgesics is a general property of the narcotic antagonists.", "PMID": 976325}
{"id": "pubmed23n0032_10676", "title": "The anticonvulsant activity of ketamine agains siezures induced by pentylenetetrazol and mercaptopropionic acid.", "content": "The activity of the dissociative anaesthetics ketamine and gamma-hydroxybutyrate against seizures induced by mercaptopropionate and pentylenetetrazol have been determined. Ketamine (90 mg/kg) prevented the seizures induced by both convulsants, but gamma-hydroxybutyrate had negligible anticonvulsant activity. Mercaptopropionate (150 mg/kg) produced a rapid fall in whole brain glutamate decarboxylase activity which correlated with the onset of convulsions. Ketamine given prior to the mercaptopropionate prevented the convulsions, but had no effect on the reduction of enzyme activity. It was concluded that although ketamine was an anticonvulsant it did not act by preventing the inhibition of glutamate decarboxylase responsible for mercaptopropionate-induced convulsions.", "contents": "The anticonvulsant activity of ketamine agains siezures induced by pentylenetetrazol and mercaptopropionic acid. The activity of the dissociative anaesthetics ketamine and gamma-hydroxybutyrate against seizures induced by mercaptopropionate and pentylenetetrazol have been determined. Ketamine (90 mg/kg) prevented the seizures induced by both convulsants, but gamma-hydroxybutyrate had negligible anticonvulsant activity. Mercaptopropionate (150 mg/kg) produced a rapid fall in whole brain glutamate decarboxylase activity which correlated with the onset of convulsions. Ketamine given prior to the mercaptopropionate prevented the convulsions, but had no effect on the reduction of enzyme activity. It was concluded that although ketamine was an anticonvulsant it did not act by preventing the inhibition of glutamate decarboxylase responsible for mercaptopropionate-induced convulsions.", "PMID": 976326}
{"id": "pubmed23n0032_10677", "title": "A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle-stimulating substances.", "content": "The classical superfusion technique was modified by immersing the isolated assay tissues in mineral oil, making it possible to superfuse the preparations through the oil with a drip of Krebs solution at very low flow rates (0.1 - 0.2 ml/min). In this way the sensitivity of the preparation for the detection and quantitation of endogenous biologically active substances was extended to the picogram level.", "contents": "A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle-stimulating substances. The classical superfusion technique was modified by immersing the isolated assay tissues in mineral oil, making it possible to superfuse the preparations through the oil with a drip of Krebs solution at very low flow rates (0.1 - 0.2 ml/min). In this way the sensitivity of the preparation for the detection and quantitation of endogenous biologically active substances was extended to the picogram level.", "PMID": 976327}
{"id": "pubmed23n0032_10678", "title": "Histamine receptor-mediated calcium ion movement in a receptor-rich microsomal fraction.", "content": "The histamine receptor-rich microsomal fraction from the longitudinal muscle of cat small intestine, which consists of membrane vesicles, was loaded with 45Ca ions and the Ca ion efflux from it was examined. Histamine increased the efflux of 45Ca ions. The effect of histamine was blocked by diphenhydramine. Histamine had no effect on the histamine receptor-free microsomal fraction from dog small intestine muscle. This suggests that the ion movement may be mediated by histamine receptors.", "contents": "Histamine receptor-mediated calcium ion movement in a receptor-rich microsomal fraction. The histamine receptor-rich microsomal fraction from the longitudinal muscle of cat small intestine, which consists of membrane vesicles, was loaded with 45Ca ions and the Ca ion efflux from it was examined. Histamine increased the efflux of 45Ca ions. The effect of histamine was blocked by diphenhydramine. Histamine had no effect on the histamine receptor-free microsomal fraction from dog small intestine muscle. This suggests that the ion movement may be mediated by histamine receptors.", "PMID": 976328}
{"id": "pubmed23n0032_10679", "title": "The dependence of the blood level of the oxime HS-6 on the severity of organophosphate poisoning.", "content": "Atropinised anaesthetised rats were injected i.v. with 4, 6 or 8 X LD50 of the organophosphorous anticholinesterase soman. Subsequent treatment with one dose of HS-6 (100 mg/kg, i.v.) delayed respiratory failure by 1 h or more; a further postponement was obtained when an additional HS-6 infusion was given. At the same infusion rate, HS-6 blood levels after 4 X LD50 soman remained stationary, but rose after the higher soman doses. The rise was greater the higher the soman dose had been. This rapid rise in oxime blood levels after high doses of organophosphate may seriously complicate therapy.", "contents": "The dependence of the blood level of the oxime HS-6 on the severity of organophosphate poisoning. Atropinised anaesthetised rats were injected i.v. with 4, 6 or 8 X LD50 of the organophosphorous anticholinesterase soman. Subsequent treatment with one dose of HS-6 (100 mg/kg, i.v.) delayed respiratory failure by 1 h or more; a further postponement was obtained when an additional HS-6 infusion was given. At the same infusion rate, HS-6 blood levels after 4 X LD50 soman remained stationary, but rose after the higher soman doses. The rise was greater the higher the soman dose had been. This rapid rise in oxime blood levels after high doses of organophosphate may seriously complicate therapy.", "PMID": 976329}
{"id": "pubmed23n0032_10680", "title": "Determination of clonazepam (\"Rivotril\" or \"Ro 5-4023\") in plasma by gas chromatography using an internal standard.", "content": "A method has been developed for gas-chromatographic determination of clonazepam (\"Rivotril\" or \"Ro 5-4023\") in plasma, using methyl-clonazepam (\"Ro 4082\") as an internal standard. Following extraction of the benzodiazepines and hydrolysis, the benzophenones are analyzed by gas-chromatography, using a glass column filled with 3% OV 225 on Gas Chrom Q and a 63Ni electron-capture detector. The technique has good selectivity. The limit of sensitivity is less than 1 ng/ml of plasma. Using this method the plasma kinetics of clonazepam may be studied in man and the correlations between plasma levels and therapeutic activity investigated. It is also available for toxicological analysis, as well as pharmacovigilance purpose. The same internal standard and a similar method can also be used for the determination of flunitrazpem (\"Rohypnol\" or \"Ro 5-4200\") and its major metabolite (N-desmethyl-flunitrazepam) in plasma.", "contents": "Determination of clonazepam (\"Rivotril\" or \"Ro 5-4023\") in plasma by gas chromatography using an internal standard. A method has been developed for gas-chromatographic determination of clonazepam (\"Rivotril\" or \"Ro 5-4023\") in plasma, using methyl-clonazepam (\"Ro 4082\") as an internal standard. Following extraction of the benzodiazepines and hydrolysis, the benzophenones are analyzed by gas-chromatography, using a glass column filled with 3% OV 225 on Gas Chrom Q and a 63Ni electron-capture detector. The technique has good selectivity. The limit of sensitivity is less than 1 ng/ml of plasma. Using this method the plasma kinetics of clonazepam may be studied in man and the correlations between plasma levels and therapeutic activity investigated. It is also available for toxicological analysis, as well as pharmacovigilance purpose. The same internal standard and a similar method can also be used for the determination of flunitrazpem (\"Rohypnol\" or \"Ro 5-4200\") and its major metabolite (N-desmethyl-flunitrazepam) in plasma.", "PMID": 976330}
{"id": "pubmed23n0032_10681", "title": "[Interference of minovine (a Vinca minor alkaloid) on hospital emergency toxicologic tests for derivatives of 5H-dibenzo(b,f)azepine].", "content": "The authors report the possible interference due to minovine when seeking tricyclic antidepressant derivatives in biological material or pharmaceutical substances. However, their work has shown that kinetic studies using absorption spectrophotometry of the colouration obtained with phosphoric-ceric reagent and thin layer adsorbant chromatography make it possible to eliminate the doubt and identify the Vinca-Minor alkaloid.", "contents": "[Interference of minovine (a Vinca minor alkaloid) on hospital emergency toxicologic tests for derivatives of 5H-dibenzo(b,f)azepine]. The authors report the possible interference due to minovine when seeking tricyclic antidepressant derivatives in biological material or pharmaceutical substances. However, their work has shown that kinetic studies using absorption spectrophotometry of the colouration obtained with phosphoric-ceric reagent and thin layer adsorbant chromatography make it possible to eliminate the doubt and identify the Vinca-Minor alkaloid.", "PMID": 976331}
{"id": "pubmed23n0032_10682", "title": "[The relationship between the course of bismuth encephalopathy and the amount of bismuth in blood and urine].", "content": "Study of the course of bismuth encephalopathy following the ingestion of a bismuth salt in relation to blood and urinary bismuth levels indicates a close parallel between the improvement in the clinical picture and the decrease in the degree of toxic impregnation of the body. This confirms the direct responsibility of bismuth for the disorder. Regression of myocolonic movements goes with the fall in blood bismuth levels, whilst improvement in the confusional syndrome is aomewhat delayed. There is a significant correlation between bismuth levels in the blood and urine. The excretion of bismuth is slow, and the persistence of blood and urinary bismuth levels higher than normal for several weeks after the acute phases and the interruption of the ingestion of bismuth salts reflects the existence of stable bonds between the metal and cellular constituents. The authors have observed a non-negligeable solubility of bismuth sub-nitrate in certain drinking waters, which brings up the hypothesis of a possible role of the hydrosobulity of bismuth in the pathogenesis of the intoxication.", "contents": "[The relationship between the course of bismuth encephalopathy and the amount of bismuth in blood and urine]. Study of the course of bismuth encephalopathy following the ingestion of a bismuth salt in relation to blood and urinary bismuth levels indicates a close parallel between the improvement in the clinical picture and the decrease in the degree of toxic impregnation of the body. This confirms the direct responsibility of bismuth for the disorder. Regression of myocolonic movements goes with the fall in blood bismuth levels, whilst improvement in the confusional syndrome is aomewhat delayed. There is a significant correlation between bismuth levels in the blood and urine. The excretion of bismuth is slow, and the persistence of blood and urinary bismuth levels higher than normal for several weeks after the acute phases and the interruption of the ingestion of bismuth salts reflects the existence of stable bonds between the metal and cellular constituents. The authors have observed a non-negligeable solubility of bismuth sub-nitrate in certain drinking waters, which brings up the hypothesis of a possible role of the hydrosobulity of bismuth in the pathogenesis of the intoxication.", "PMID": 976332}
{"id": "pubmed23n0032_10683", "title": "[Specific assessment of the cerebral and cardiac effects of asphyxic hypoxia. Application to the study of substances with protective activity].", "content": "The influence of asphyxia on electrocorticogram (E. Co. G.) and electrocardiogram (E. C. G.) has been studied in curarized rats. The delay between the heightening of the ST component of E. C. G. and the disappearance of E. Co. G. pattern was measured. It provided more useful information on cerebral survival during asphyxia than the only study of E. Co. G. did. This delay was increased (cerebral antihypoxic effect) after intravenous treatment with pyritinol (50 mg/kg), vincamine (5 mg/kg) or propranolol (1 mg/kg), but it was not influenced by pentobarbital (60 mg/kg).", "contents": "[Specific assessment of the cerebral and cardiac effects of asphyxic hypoxia. Application to the study of substances with protective activity]. The influence of asphyxia on electrocorticogram (E. Co. G.) and electrocardiogram (E. C. G.) has been studied in curarized rats. The delay between the heightening of the ST component of E. C. G. and the disappearance of E. Co. G. pattern was measured. It provided more useful information on cerebral survival during asphyxia than the only study of E. Co. G. did. This delay was increased (cerebral antihypoxic effect) after intravenous treatment with pyritinol (50 mg/kg), vincamine (5 mg/kg) or propranolol (1 mg/kg), but it was not influenced by pentobarbital (60 mg/kg).", "PMID": 976333}
{"id": "pubmed23n0032_10684", "title": "[Continuous inhalation of small amounts of formaldehyde: experimental study in the rat].", "content": "The results of experiments on the Rat, about the effects of continuous inhalation of 1.60, 4.55 and 8,07 ppm of formaldehyde, are reported: 150 SPF male rats were observed during one and an half to three months. These experiments concerned the clinical observation of the rats, their body weight and food intake, the relative weights of their lungs, liver, kidneys and spleen, the number and activity of their alveolar macrophages harvested by pulmonary washing. 1degree At 1.60 ppm it was only observed a yellowing of the hair of the intoxicated rats. 2degrees At 4.55 ppm, besides, the body weight of the intoxicated animals became significantly lower than the controls. 3degrees At 8.07 ppm, in addition to those differences, it appeared signs of irritation in the upper respiratory tract and in the eyes of the intoxicated rats, a significant underfeeding and a percentage of liver weight lower than the controls. Besides, the relative number and the phagocytic ability of alveolar macrophages of intoxicated rats was significantly lower than the controls. It was not observed the phenomena of adaptation previously described in the experimental rat intoxication by acrolein.", "contents": "[Continuous inhalation of small amounts of formaldehyde: experimental study in the rat]. The results of experiments on the Rat, about the effects of continuous inhalation of 1.60, 4.55 and 8,07 ppm of formaldehyde, are reported: 150 SPF male rats were observed during one and an half to three months. These experiments concerned the clinical observation of the rats, their body weight and food intake, the relative weights of their lungs, liver, kidneys and spleen, the number and activity of their alveolar macrophages harvested by pulmonary washing. 1degree At 1.60 ppm it was only observed a yellowing of the hair of the intoxicated rats. 2degrees At 4.55 ppm, besides, the body weight of the intoxicated animals became significantly lower than the controls. 3degrees At 8.07 ppm, in addition to those differences, it appeared signs of irritation in the upper respiratory tract and in the eyes of the intoxicated rats, a significant underfeeding and a percentage of liver weight lower than the controls. Besides, the relative number and the phagocytic ability of alveolar macrophages of intoxicated rats was significantly lower than the controls. It was not observed the phenomena of adaptation previously described in the experimental rat intoxication by acrolein.", "PMID": 976334}
{"id": "pubmed23n0032_10685", "title": "[Treatment of acute lithium poisoning with forced furosemide diuresis (physicopharmacodynamic bases)].", "content": "The basis of the treatment of acute lithium intoxication is clearance of the poison from the blood. Lithium and sodium ions being excreted under the influence of furosemide, the authors feel, in the light of one case, that forced diuresis, non-agressive, using furosemide appears to be the method of choice.", "contents": "[Treatment of acute lithium poisoning with forced furosemide diuresis (physicopharmacodynamic bases)]. The basis of the treatment of acute lithium intoxication is clearance of the poison from the blood. Lithium and sodium ions being excreted under the influence of furosemide, the authors feel, in the light of one case, that forced diuresis, non-agressive, using furosemide appears to be the method of choice.", "PMID": 976335}
{"id": "pubmed23n0032_10686", "title": "Fibroblast precursors in normal and irradiated mouse hematopoietic organs.", "content": "Using the in vitro colony assay, clonogenic fibroblast precursor cells (CFU-F) were detected in the bone marrow, spleen and thymus from adult mice. The survival curve for CFU-F of mouse bone marrow irradiated in vitro has a D0 of 220 r. Regeneration of bone marrow CFU-F after whole-body irradiation with 150 r is characterized by a marked secondary loss and post-irradiation lag and dip, lasting 6 days, followed by return to normal values by about the 25th day. This pattern of post-radiation recovery of CFU-F is similar to that of the CFU-s. In addition, during the first 6 hours following irradiation the number of CFU-F increased approximately twofold.", "contents": "Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Using the in vitro colony assay, clonogenic fibroblast precursor cells (CFU-F) were detected in the bone marrow, spleen and thymus from adult mice. The survival curve for CFU-F of mouse bone marrow irradiated in vitro has a D0 of 220 r. Regeneration of bone marrow CFU-F after whole-body irradiation with 150 r is characterized by a marked secondary loss and post-irradiation lag and dip, lasting 6 days, followed by return to normal values by about the 25th day. This pattern of post-radiation recovery of CFU-F is similar to that of the CFU-s. In addition, during the first 6 hours following irradiation the number of CFU-F increased approximately twofold.", "PMID": 976387}
{"id": "pubmed23n0032_10687", "title": "Techniques for induction of neutropenia and granulocytosis in rats.", "content": "After a single administration of vinblastine, rats develop profound neutropenia. The agranulocytosis lasts 3 days, and it is observed on the third, fourth and fifth day after vinblastine administration. The granulocytosis that develops on days 7-14 after vinblastine administration was significantly increased when androgenic steroids were administered. Deca-Durabolin induced greater granulocytosis than testosterone. The peak values were observed 10 and 12 days, respectively, after drug administration. All values of WBC's, granulocytes and hematocrit ratios were obtained in unanesthetized, unrestrained rats from an aortic cannula implanted at least 10 days prior to the experiment.", "contents": "Techniques for induction of neutropenia and granulocytosis in rats. After a single administration of vinblastine, rats develop profound neutropenia. The agranulocytosis lasts 3 days, and it is observed on the third, fourth and fifth day after vinblastine administration. The granulocytosis that develops on days 7-14 after vinblastine administration was significantly increased when androgenic steroids were administered. Deca-Durabolin induced greater granulocytosis than testosterone. The peak values were observed 10 and 12 days, respectively, after drug administration. All values of WBC's, granulocytes and hematocrit ratios were obtained in unanesthetized, unrestrained rats from an aortic cannula implanted at least 10 days prior to the experiment.", "PMID": 976388}
{"id": "pubmed23n0032_10688", "title": "The influence of steroids on erythropoiesis in mouse fetal liver cell cultures: relevance of cell cycle state.", "content": "It has been shown that erythropoietin-mediated stimulation of heme synthesis in mouse fetal liver cells in vitro is correlated with hydroxyurea sensitivity. Assuming that OHU is specifically cytotoxic for cells in DNA synthesis this suggests that erythropoietin sensitivity may be related to this phase of the cell cycle. The direct effects of 19-nortestosterone and 3beta-hydroxy-5beta-pregnan-20-one on heme synthesis correlated with the capacity of these steroids to initiate DNA synthesis. It is suggested that the ability of these steroids to increase the proportion of cells in the Ep-sensitive phase of the cell cycle is probably the mechanism responsible for the erythropoietic effects of these agents. 17beta-hydroxy-5alpha-androstan-3-one appears to have a different mode of action since it has only minimal effects on heme synthesis and did not increase hydroxyurea sensitivity.", "contents": "The influence of steroids on erythropoiesis in mouse fetal liver cell cultures: relevance of cell cycle state. It has been shown that erythropoietin-mediated stimulation of heme synthesis in mouse fetal liver cells in vitro is correlated with hydroxyurea sensitivity. Assuming that OHU is specifically cytotoxic for cells in DNA synthesis this suggests that erythropoietin sensitivity may be related to this phase of the cell cycle. The direct effects of 19-nortestosterone and 3beta-hydroxy-5beta-pregnan-20-one on heme synthesis correlated with the capacity of these steroids to initiate DNA synthesis. It is suggested that the ability of these steroids to increase the proportion of cells in the Ep-sensitive phase of the cell cycle is probably the mechanism responsible for the erythropoietic effects of these agents. 17beta-hydroxy-5alpha-androstan-3-one appears to have a different mode of action since it has only minimal effects on heme synthesis and did not increase hydroxyurea sensitivity.", "PMID": 976389}
{"id": "pubmed23n0032_10689", "title": "Effect of androgens on maturation and metabolism of erythroid tissue.", "content": "Specific changes taking place in the erythroid tissue following depletion or replacement of androgens were studied in rats. The reduction of testosterone levels in blood of orchiectomized animals did occur in conjunction with a decline of erythrocyte glucose-6-phosphate (G6P) and lactate levels. No evidence of anemia was observed. The subcutaneous administration of testosterone propionate (16.0 mg/kg) to orchiectomized rats restored, within 12 hours, blood testosterone levels as well as erythrocyte G6P levels and lactate production. The in vitro incorporation of glucose-1-14C into rat erythrocytes incubated with testosterone was comparable to that of control cells. A radioautographic study of rat erythroid marrow pulsed with glucose-1-14C showed a lower labeling when testosterone propionate was administered. The authors conclude that testosterone does directly affect glucose metabolism of erythroid cells, via the pentose shunt pathway. The possible role of the androgen-dependent enhancement of erythroid glycolysis is discussed in relation to the function of testosterone receptocytes present in marrow cells and a 17beta-hydroxysteroid dehydrogenase present in erythrocytes.", "contents": "Effect of androgens on maturation and metabolism of erythroid tissue. Specific changes taking place in the erythroid tissue following depletion or replacement of androgens were studied in rats. The reduction of testosterone levels in blood of orchiectomized animals did occur in conjunction with a decline of erythrocyte glucose-6-phosphate (G6P) and lactate levels. No evidence of anemia was observed. The subcutaneous administration of testosterone propionate (16.0 mg/kg) to orchiectomized rats restored, within 12 hours, blood testosterone levels as well as erythrocyte G6P levels and lactate production. The in vitro incorporation of glucose-1-14C into rat erythrocytes incubated with testosterone was comparable to that of control cells. A radioautographic study of rat erythroid marrow pulsed with glucose-1-14C showed a lower labeling when testosterone propionate was administered. The authors conclude that testosterone does directly affect glucose metabolism of erythroid cells, via the pentose shunt pathway. The possible role of the androgen-dependent enhancement of erythroid glycolysis is discussed in relation to the function of testosterone receptocytes present in marrow cells and a 17beta-hydroxysteroid dehydrogenase present in erythrocytes.", "PMID": 976390}
{"id": "pubmed23n0032_10690", "title": "Effects of radiation on hematopoietic stroma.", "content": "The effects of x-irradiation on the CFU-S and on the hematopoietic stroma (HS-P) were studied and compared. Exposure to 300 rad reduced the CFU-S population to less than 1% of normal; by contrast more than 500 rad was required to detectably damage the HS-P. Although exposture to 500 rad did not detectably affect HS-P function, a second exposure to 500 rad as much as 4 weeks later did appreciably affect the HS-P. In addition, whereas the CFU-S returned to the pre-irradiation level within 6 weeks, the HS-P did not significantly recover. From these data we conclude that, with respect to the initial impact and exposure dose, HS-P is more radioresistant than CFU-S, but recovery from radiation induced damage of HS-P occurs slowly or does not take place.", "contents": "Effects of radiation on hematopoietic stroma. The effects of x-irradiation on the CFU-S and on the hematopoietic stroma (HS-P) were studied and compared. Exposure to 300 rad reduced the CFU-S population to less than 1% of normal; by contrast more than 500 rad was required to detectably damage the HS-P. Although exposture to 500 rad did not detectably affect HS-P function, a second exposure to 500 rad as much as 4 weeks later did appreciably affect the HS-P. In addition, whereas the CFU-S returned to the pre-irradiation level within 6 weeks, the HS-P did not significantly recover. From these data we conclude that, with respect to the initial impact and exposure dose, HS-P is more radioresistant than CFU-S, but recovery from radiation induced damage of HS-P occurs slowly or does not take place.", "PMID": 976391}
{"id": "pubmed23n0032_10691", "title": "Time-lapse characterization of erythrocytic colony-forming cells in plasma cultures.", "content": "The development of erythrocytic colonies in plasma cultures of C3H/Bi mouse fetal liver cells stimulated with erythropoietin was recorded by time-lapse cinemicrography, in order to investigate the nature of the colony-forming unit (CFU-E) from which they arose. Each colony was found to develop from a single cell, which in most cases underwent two divisions before acquiring hemoglobin, as detected by absorption in the Soret band. Less frequently, there were one or three divisions before hemoglobin appeared at 11.7 to 29.2 h. The diameter of the colony-forming cells in G2 was 16.0+/-1.3 mum (mean+/-SD). Daughter cells in each colony had similar generation times, with a TG of 11.0+/-2.2 h (mean+/-SD) for the first generation of colony cells. Evidence is presented that while some of the colony-forming cells were triggered into division by the erythropoietin added at plating, others had already been stimulated by endogenous erythropoietin in the fetus.", "contents": "Time-lapse characterization of erythrocytic colony-forming cells in plasma cultures. The development of erythrocytic colonies in plasma cultures of C3H/Bi mouse fetal liver cells stimulated with erythropoietin was recorded by time-lapse cinemicrography, in order to investigate the nature of the colony-forming unit (CFU-E) from which they arose. Each colony was found to develop from a single cell, which in most cases underwent two divisions before acquiring hemoglobin, as detected by absorption in the Soret band. Less frequently, there were one or three divisions before hemoglobin appeared at 11.7 to 29.2 h. The diameter of the colony-forming cells in G2 was 16.0+/-1.3 mum (mean+/-SD). Daughter cells in each colony had similar generation times, with a TG of 11.0+/-2.2 h (mean+/-SD) for the first generation of colony cells. Evidence is presented that while some of the colony-forming cells were triggered into division by the erythropoietin added at plating, others had already been stimulated by endogenous erythropoietin in the fetus.", "PMID": 976393}
{"id": "pubmed23n0032_10692", "title": "Fulminant Purkinje cell death following axotomy and its use for analysis of the dendritic arborization.", "content": "Young and adult cats were operated upon and a number of the vermal cerebellar folia were either transected with a vertical incision or isolated by a horizontal cut. In the proximity of the lesion, Purkinje cell bodies and their dendritic trees became stainable with the Fink-Heimer method. Electron microscopy of the silver stained sections show that the argyrophilic Purkinje neurons undergo an electron dense type of degeneration. Stellate cell dendrites adjacent to the degenerating Purkinje trees are normal, suggesting that the cause of cell death is axotomy close to the perikaryon rather than direct injury. The retrograde Purkinje cell degeneration is fulminant since it is evident 6 hours after the lesion. In Fink-Heimer stained sections the entire dendritic tree is impregnated 1-3 days after the lesion. 4-10 days post-operatively, the flattened dendritic tree becomes fragmented and is partially phagocytized. The silver stained arborizations are approximately 280 mu in width and have an uneven thickness (8-16 mu). In longitudinal and horizontal silver stained sections of lesioned cerebellar folia, uninterrupted fields of degenerating Purkinje cell arborizations can be seen, suggesting that the arborizations overlap. The overlap was demonstrated in electron micrographs of single degenerating arborizations surrounded by normal dendritic trees. The degree of overlap varies with the thickness of the arborization and is in the order of 1-2 mu. This approach indicates that each Purkinje tree occupies an exclusive sheet of molecular layer 8 mu thick and may overlap for as much as 2 mu on each side with neighboring trees. The average thickness of the Purkinje tree is approximately 12 mu.", "contents": "Fulminant Purkinje cell death following axotomy and its use for analysis of the dendritic arborization. Young and adult cats were operated upon and a number of the vermal cerebellar folia were either transected with a vertical incision or isolated by a horizontal cut. In the proximity of the lesion, Purkinje cell bodies and their dendritic trees became stainable with the Fink-Heimer method. Electron microscopy of the silver stained sections show that the argyrophilic Purkinje neurons undergo an electron dense type of degeneration. Stellate cell dendrites adjacent to the degenerating Purkinje trees are normal, suggesting that the cause of cell death is axotomy close to the perikaryon rather than direct injury. The retrograde Purkinje cell degeneration is fulminant since it is evident 6 hours after the lesion. In Fink-Heimer stained sections the entire dendritic tree is impregnated 1-3 days after the lesion. 4-10 days post-operatively, the flattened dendritic tree becomes fragmented and is partially phagocytized. The silver stained arborizations are approximately 280 mu in width and have an uneven thickness (8-16 mu). In longitudinal and horizontal silver stained sections of lesioned cerebellar folia, uninterrupted fields of degenerating Purkinje cell arborizations can be seen, suggesting that the arborizations overlap. The overlap was demonstrated in electron micrographs of single degenerating arborizations surrounded by normal dendritic trees. The degree of overlap varies with the thickness of the arborization and is in the order of 1-2 mu. This approach indicates that each Purkinje tree occupies an exclusive sheet of molecular layer 8 mu thick and may overlap for as much as 2 mu on each side with neighboring trees. The average thickness of the Purkinje tree is approximately 12 mu.", "PMID": 976394}
{"id": "pubmed23n0032_10693", "title": "Eye tracking of self-moved targets in the absence of vision.", "content": "Smooth pursuit eye movements have been described as resulting from the tracking of self-moved targets in total darkness. This study investigated the nature of the signal responsible for the release of smooth pursuit in this particular situation. Simultaneous monitoring of eye and hand positions shows that in total darkness smooth pursuit can only be released if the imagined target is either passively or actively moved by the subject's hand. An ischaemic block applied at the level of the biceps allowed us to selectively remove the afferent signal preferentially to the efferent copy in tasks involving eye tracking of an imaginary target actively or passively moved. The results show that an afferent signal was necessary and sufficient to release smooth pursuit, whereas the efferent copy alone could not trigger smooth pursuit. However, the efferent copy could play an important role in the phase relationship (prediction) between eye and finger events and in the activation of the concomitantly active saccadic system. Analysis of the eye movement characteristics, in various non-visually guided, load-affected situations, suggested that the main input to the smooth pursuit system was derived, in a non-graded way, from the position detector activation of the target-moving structure.", "contents": "Eye tracking of self-moved targets in the absence of vision. Smooth pursuit eye movements have been described as resulting from the tracking of self-moved targets in total darkness. This study investigated the nature of the signal responsible for the release of smooth pursuit in this particular situation. Simultaneous monitoring of eye and hand positions shows that in total darkness smooth pursuit can only be released if the imagined target is either passively or actively moved by the subject's hand. An ischaemic block applied at the level of the biceps allowed us to selectively remove the afferent signal preferentially to the efferent copy in tasks involving eye tracking of an imaginary target actively or passively moved. The results show that an afferent signal was necessary and sufficient to release smooth pursuit, whereas the efferent copy alone could not trigger smooth pursuit. However, the efferent copy could play an important role in the phase relationship (prediction) between eye and finger events and in the activation of the concomitantly active saccadic system. Analysis of the eye movement characteristics, in various non-visually guided, load-affected situations, suggested that the main input to the smooth pursuit system was derived, in a non-graded way, from the position detector activation of the target-moving structure.", "PMID": 976395}
{"id": "pubmed23n0032_10694", "title": "Quantitative ultrastructural observations on the inhibited recovery of the hypoglossal nucleus from the axotomy response when regeneration of the hypoglossal nerve is prevented.", "content": "Electron micrographs of the left hypoglossal nucleus were quantitatively analyzed in adult rats 52 to 98 days after transection of the left hypoglossal nerve and implantation of the proximal stump into the already innervated ipsilateral sternomastoid muscle, a procedure which prevented the transected nerve from regenerating. Many presynaptic boutons with clear spherical synaptic vesicles and symmetrical synapses were lost from the injured perikarya and dendrites. Some perikarya and dendrites (and, rarely, boutons) became electron dense, and astrocyte or microglial sheaths partly surrounded them. Numbers of dendrite profiles in the neuropil decreased. These statistically significant effects persisted throughout the postoperative period, whereas after axotomy with unimpeded nerve regeneration, these features would have returned to normal by 84 days postoperatively. It was therefore suggested that their recovery depended upon successful regeneration and reconnection of the hypoglossal nerve with the tongue. Subsurface cisterns, and profiles containing unusual inclusions, were numerically normal 52 to 98 days postoperatively, so it was suggested that their early response and recovery after simple axotomy might be entirely dependent on nerve disconnection and not on nerve reconnection.", "contents": "Quantitative ultrastructural observations on the inhibited recovery of the hypoglossal nucleus from the axotomy response when regeneration of the hypoglossal nerve is prevented. Electron micrographs of the left hypoglossal nucleus were quantitatively analyzed in adult rats 52 to 98 days after transection of the left hypoglossal nerve and implantation of the proximal stump into the already innervated ipsilateral sternomastoid muscle, a procedure which prevented the transected nerve from regenerating. Many presynaptic boutons with clear spherical synaptic vesicles and symmetrical synapses were lost from the injured perikarya and dendrites. Some perikarya and dendrites (and, rarely, boutons) became electron dense, and astrocyte or microglial sheaths partly surrounded them. Numbers of dendrite profiles in the neuropil decreased. These statistically significant effects persisted throughout the postoperative period, whereas after axotomy with unimpeded nerve regeneration, these features would have returned to normal by 84 days postoperatively. It was therefore suggested that their recovery depended upon successful regeneration and reconnection of the hypoglossal nerve with the tongue. Subsurface cisterns, and profiles containing unusual inclusions, were numerically normal 52 to 98 days postoperatively, so it was suggested that their early response and recovery after simple axotomy might be entirely dependent on nerve disconnection and not on nerve reconnection.", "PMID": 976396}
{"id": "pubmed23n0032_10695", "title": "Age dependence of the effect of squint on cells in kittens' visual cortex.", "content": "The period of susceptibility of the visual cortex of kittens to the effect of squint is limited to the first three postnatal months. The reduction of binocularity found in these kittens as reflected by the distribution of neurons according to their ocular dominance is especially emphasized in animals operated on between the ages of 4-7 weeks in comparison to animals operated on between the ages of 8-11 weeks. The proportion of monocularly dominated neurons within the first three postnatal months is significantly (0.05 greater than p greater than 0.025) reduced with age. Similar effect on cortical neurons was found for animals who were under the influence of squint for 6 weeks or 15 months and for animals with wide range of deviation angles.", "contents": "Age dependence of the effect of squint on cells in kittens' visual cortex. The period of susceptibility of the visual cortex of kittens to the effect of squint is limited to the first three postnatal months. The reduction of binocularity found in these kittens as reflected by the distribution of neurons according to their ocular dominance is especially emphasized in animals operated on between the ages of 4-7 weeks in comparison to animals operated on between the ages of 8-11 weeks. The proportion of monocularly dominated neurons within the first three postnatal months is significantly (0.05 greater than p greater than 0.025) reduced with age. Similar effect on cortical neurons was found for animals who were under the influence of squint for 6 weeks or 15 months and for animals with wide range of deviation angles.", "PMID": 976397}
{"id": "pubmed23n0032_10696", "title": "The synaptic organization of the cerebello-olivary circuit.", "content": "Section of the superior cerebellar peduncle just rostral to the deep cerebellar nuclei results in degenerating axon terminals within the contralateral inferior olive. The nuclear origin of this fiber system and its distribution within the subdivisions of the inferior olive were described in a companion study (Martin et al., 1976). Precise localization of these degenerating terminals within the nucleus was accomplished by the examination of 1 mu plastic sections cut from each tissue block prior to thin sectioning. Degenerating axon terminals are present in all the nuclear subdivisions and when seen with the electron microscope they frequently are localized in the previously described synaptic clusters (King, 1976). These terminals demonstrate an electron dense reaction at survival times of 2 and 3 days. By day 4, they are shrunken and irregular in shape, and typically are surrounded by astrocyte processes. Cerebello-olivary axon terminals measure 1-3 mu, contain spherical, clear synaptic vesicles and typically contact spiny appendages within the synaptic clusters (glomeruli). Thus, we have demonstrated that one of the primary axon systems which terminates within the synaptic clusters is from the cerebellar nuclei. We have yet to determine the origins of the remaining terminals within the synaptic clusters which include endings with either smaller spherical, pleomorphic or numerous dense core vesicles.", "contents": "The synaptic organization of the cerebello-olivary circuit. Section of the superior cerebellar peduncle just rostral to the deep cerebellar nuclei results in degenerating axon terminals within the contralateral inferior olive. The nuclear origin of this fiber system and its distribution within the subdivisions of the inferior olive were described in a companion study (Martin et al., 1976). Precise localization of these degenerating terminals within the nucleus was accomplished by the examination of 1 mu plastic sections cut from each tissue block prior to thin sectioning. Degenerating axon terminals are present in all the nuclear subdivisions and when seen with the electron microscope they frequently are localized in the previously described synaptic clusters (King, 1976). These terminals demonstrate an electron dense reaction at survival times of 2 and 3 days. By day 4, they are shrunken and irregular in shape, and typically are surrounded by astrocyte processes. Cerebello-olivary axon terminals measure 1-3 mu, contain spherical, clear synaptic vesicles and typically contact spiny appendages within the synaptic clusters (glomeruli). Thus, we have demonstrated that one of the primary axon systems which terminates within the synaptic clusters is from the cerebellar nuclei. We have yet to determine the origins of the remaining terminals within the synaptic clusters which include endings with either smaller spherical, pleomorphic or numerous dense core vesicles.", "PMID": 976398}
{"id": "pubmed23n0032_10697", "title": "Unusually large receptive fields in cats with restricted visual experience.", "content": "The receptive fields of striate cortex neurons were analyzed in cats which had restricted or no visual experience. Two groups of animals were investigated: 1. cats which were deprived from contour vision over variable periods of time up to 1 year and 2. kittens whose visual experience was restricted to vertically oriented gratings of constant spatial frequency which moved unidirectionally at a fixed distance in front of the restrained animals. In both preparations exceedingly large receptive fields (up to 20 degrees in diameter) were encountered, especially in cells located in supragranular layers. These large receptive fields never extended over more than 2 degrees into the ipsilateral hemifield. Their sensitivity profile was frequently asymmetric and contained discontinuities. Many of these large receptive fields consisted of several excitatory subregions which were separated from each other by as much as 15 degrees. Often but not always the most sensitive area was located where the retinotopic map predicted the receptive field center. The orientation and direction selectivity and also the angular separation of such multiple excitatory bands often matched precisely the orientation, direction and spatial frequency of the experienced moving grating. In other fields with multiple excitatory subregions such a correspondence could not be established; the various subregions could even have different orientation and direction selectivities. From these unconventional receptive fields it is concluded that the function of cat striate cortex is not confined to a point by point analysis of the visual field in retinotopically organized and functionally isolated columns.", "contents": "Unusually large receptive fields in cats with restricted visual experience. The receptive fields of striate cortex neurons were analyzed in cats which had restricted or no visual experience. Two groups of animals were investigated: 1. cats which were deprived from contour vision over variable periods of time up to 1 year and 2. kittens whose visual experience was restricted to vertically oriented gratings of constant spatial frequency which moved unidirectionally at a fixed distance in front of the restrained animals. In both preparations exceedingly large receptive fields (up to 20 degrees in diameter) were encountered, especially in cells located in supragranular layers. These large receptive fields never extended over more than 2 degrees into the ipsilateral hemifield. Their sensitivity profile was frequently asymmetric and contained discontinuities. Many of these large receptive fields consisted of several excitatory subregions which were separated from each other by as much as 15 degrees. Often but not always the most sensitive area was located where the retinotopic map predicted the receptive field center. The orientation and direction selectivity and also the angular separation of such multiple excitatory bands often matched precisely the orientation, direction and spatial frequency of the experienced moving grating. In other fields with multiple excitatory subregions such a correspondence could not be established; the various subregions could even have different orientation and direction selectivities. From these unconventional receptive fields it is concluded that the function of cat striate cortex is not confined to a point by point analysis of the visual field in retinotopically organized and functionally isolated columns.", "PMID": 976399}
{"id": "pubmed23n0032_10698", "title": "Input from trigeminal cutaneous afferents to neurones of the inferior olive in rats.", "content": "Extracellular recordings were obtained from inferior olivary neurones of the rat. The responses of fifty neurones evoked by electrical stimulation of a branch of the trigeminal nerve were recorded. Maxillary nerve stimulation was most effective. The response was characterized by an early discharge (single spike and wave, typically with latencies between 16 and 30 msec) and a weak late discharge which followed a period of inhibition of about 100 msec. Half of the neurones responded to one branch of the trigeminal nerve only whereas the other neurons displayed a varying degree of convergence, including sometimes a convergence from limb nerves. Forty-nine olivary neurones were tested for cutaneous receptive fields. Ten out of these had small receptive fields (less than 20% of the contralateral face) and a low threshold to mechanical stimuli. Twenty neurones which had larger receptive fields responded also to low-threshold or to medium-threshold (i.e. non-nociceptive) mechanical stimuli. None of the neurones displayed receptive fields more extensive than half of the contralateral face and some of the larger fields had a small, low-threshold focus. Olivary neurones responding to electrical stimulation of trigeminal nerves or mechanical stimulation of the face were located in the medial segment of the olivary complex (dorsal accessory and principal olive). A few cells only were located in the lateral segment. It is concluded that neurones of the inferior olive receive a substantial input from trigeminal afferents and are capable of transmitting precise somatotopical information to the cerebellum.", "contents": "Input from trigeminal cutaneous afferents to neurones of the inferior olive in rats. Extracellular recordings were obtained from inferior olivary neurones of the rat. The responses of fifty neurones evoked by electrical stimulation of a branch of the trigeminal nerve were recorded. Maxillary nerve stimulation was most effective. The response was characterized by an early discharge (single spike and wave, typically with latencies between 16 and 30 msec) and a weak late discharge which followed a period of inhibition of about 100 msec. Half of the neurones responded to one branch of the trigeminal nerve only whereas the other neurons displayed a varying degree of convergence, including sometimes a convergence from limb nerves. Forty-nine olivary neurones were tested for cutaneous receptive fields. Ten out of these had small receptive fields (less than 20% of the contralateral face) and a low threshold to mechanical stimuli. Twenty neurones which had larger receptive fields responded also to low-threshold or to medium-threshold (i.e. non-nociceptive) mechanical stimuli. None of the neurones displayed receptive fields more extensive than half of the contralateral face and some of the larger fields had a small, low-threshold focus. Olivary neurones responding to electrical stimulation of trigeminal nerves or mechanical stimulation of the face were located in the medial segment of the olivary complex (dorsal accessory and principal olive). A few cells only were located in the lateral segment. It is concluded that neurones of the inferior olive receive a substantial input from trigeminal afferents and are capable of transmitting precise somatotopical information to the cerebellum.", "PMID": 976400}
{"id": "pubmed23n0032_10699", "title": "Elevated kidney glucosyltransferase activity in genetic prediabetic mice.", "content": "Glucosyltransferase activity in the renal cortex of genetic diabetic KK mice was significantly increased at 40 days of age when compared to that of Swiss albino and F1 hybrid mice. This increase in enzyme activity in the absence of glucose intolerance can be regarded as an earlier genetic marker for the diagnosis of diabetic microangiopathy.", "contents": "Elevated kidney glucosyltransferase activity in genetic prediabetic mice. Glucosyltransferase activity in the renal cortex of genetic diabetic KK mice was significantly increased at 40 days of age when compared to that of Swiss albino and F1 hybrid mice. This increase in enzyme activity in the absence of glucose intolerance can be regarded as an earlier genetic marker for the diagnosis of diabetic microangiopathy.", "PMID": 976428}
{"id": "pubmed23n0032_10700", "title": "Metabolism of 1,3,7-trimethyldihydrouric acid in the rat: new metabolic pathway of caffeine.", "content": "[1-CH3-14C] 1,3,7-trimethyldihydrouric acid which, in quantity, is the most important caffeine metabolite, was isolated and purified from the urine of rats fed with [1-CH3-14C] caffeine. The oral administration of this metabolite to rats showed that 1,3,7-trimethyldihydrouric acid was excreted unchanged in urine and was therefore an end product of caffeine metabolism. This result implies a new metabolic pathway of caffeine.", "contents": "Metabolism of 1,3,7-trimethyldihydrouric acid in the rat: new metabolic pathway of caffeine. [1-CH3-14C] 1,3,7-trimethyldihydrouric acid which, in quantity, is the most important caffeine metabolite, was isolated and purified from the urine of rats fed with [1-CH3-14C] caffeine. The oral administration of this metabolite to rats showed that 1,3,7-trimethyldihydrouric acid was excreted unchanged in urine and was therefore an end product of caffeine metabolism. This result implies a new metabolic pathway of caffeine.", "PMID": 976429}
{"id": "pubmed23n0032_10701", "title": "The desulphation of hexosamine sulphates by arylsulphatase B.", "content": "The sulphation of Carbobenzoxyglucosamine by chlorosulphonic acid resulted in formation of N-carbobenzoxyglucosamine-4,6-disulphate. UDP-galactosamine 4-sulphate and glucosamine 4,6-disulphate were the competitive inhibitors of arylsulphatase B. Arylsulphatase B can hydrolyze UDP-galactosamine 4-sulphate and glucosamine 4,6-disulphate but not galactosamine 6-sulphate.", "contents": "The desulphation of hexosamine sulphates by arylsulphatase B. The sulphation of Carbobenzoxyglucosamine by chlorosulphonic acid resulted in formation of N-carbobenzoxyglucosamine-4,6-disulphate. UDP-galactosamine 4-sulphate and glucosamine 4,6-disulphate were the competitive inhibitors of arylsulphatase B. Arylsulphatase B can hydrolyze UDP-galactosamine 4-sulphate and glucosamine 4,6-disulphate but not galactosamine 6-sulphate.", "PMID": 976430}
{"id": "pubmed23n0032_10702", "title": "[Effect of 6beta-Methoxy-9beta,10alpha-pregna-4,6-diene-3,20-dione (Ro 6-1963) on the steroid reductases in rat liver].", "content": "6beta-Methoxy-9beta,10 alpha-pregna-4,6-diene-3,20-dione inhibits the delta4-3-oxosteroid-5alpha-reduction in microsomes and the delta4-3-oxosteroid-5beta-reduction in the soluble fraction of male rat liver. The 3alpha- and 3beta-hydroxysteroid dehydrogenase are not inhibited by these substances.", "contents": "[Effect of 6beta-Methoxy-9beta,10alpha-pregna-4,6-diene-3,20-dione (Ro 6-1963) on the steroid reductases in rat liver]. 6beta-Methoxy-9beta,10 alpha-pregna-4,6-diene-3,20-dione inhibits the delta4-3-oxosteroid-5alpha-reduction in microsomes and the delta4-3-oxosteroid-5beta-reduction in the soluble fraction of male rat liver. The 3alpha- and 3beta-hydroxysteroid dehydrogenase are not inhibited by these substances.", "PMID": 976433}
{"id": "pubmed23n0032_10703", "title": "Reactions of the goldfish (Carassius auratus auratus L.) to quantified mechanical and thermal stimuli.", "content": "Goldfish can differentiate between mechanical and thermal stimuli components in combined stimulations. Reactions to heated thermode (deltaT = + 3 degrees C) differ from those to isotherm thermode by up to 30%. Additional pretension (0.5 p) increases the reaction more than double; at simultaneously varied temperature (deltaT = + 3 degrees C and + 5 degrees C), there is a further increase in reaction of 25% and 35%. The significance for the organism of the two stimuli components is discussed.", "contents": "Reactions of the goldfish (Carassius auratus auratus L.) to quantified mechanical and thermal stimuli. Goldfish can differentiate between mechanical and thermal stimuli components in combined stimulations. Reactions to heated thermode (deltaT = + 3 degrees C) differ from those to isotherm thermode by up to 30%. Additional pretension (0.5 p) increases the reaction more than double; at simultaneously varied temperature (deltaT = + 3 degrees C and + 5 degrees C), there is a further increase in reaction of 25% and 35%. The significance for the organism of the two stimuli components is discussed.", "PMID": 976435}
{"id": "pubmed23n0032_10704", "title": "Thiamine diphosphatase in rat small intestine.", "content": "TDPase is located mostly in the proximal portion of the small intestine and its activity, like that of ALPase, decreased markedly in thiamine deficiency. The decreased enzyme activities were restored after thiamine or vitamin D3. Kinetic and other studies of the purified enzyme indicated the identity of the two enzymes.", "contents": "Thiamine diphosphatase in rat small intestine. TDPase is located mostly in the proximal portion of the small intestine and its activity, like that of ALPase, decreased markedly in thiamine deficiency. The decreased enzyme activities were restored after thiamine or vitamin D3. Kinetic and other studies of the purified enzyme indicated the identity of the two enzymes.", "PMID": 976434}
{"id": "pubmed23n0032_10705", "title": "Kinetics of lymphocyte division in blood cultures studied with the BrdU-Giemsa technique.", "content": "Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 mug/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.", "contents": "Kinetics of lymphocyte division in blood cultures studied with the BrdU-Giemsa technique. Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 mug/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.", "PMID": 976436}
{"id": "pubmed23n0032_10706", "title": "The effect of sulfhydryl reagents upon the activity of 40S ribosomal subunits.", "content": "p-Chloromercuribenzoate inhibited the poly (U)-dependent binding of the Phe-tRNA to the 40S ribosomal subunit but displayed no inhibitory effect on the binding of poly (U) to the ribosome. Other sulfhydryl reagents tested, like N-ethylmaleimide and iodoacetamide, did not affect the binding of Phe-tRNA to the small ribosomal subunit.", "contents": "The effect of sulfhydryl reagents upon the activity of 40S ribosomal subunits. p-Chloromercuribenzoate inhibited the poly (U)-dependent binding of the Phe-tRNA to the 40S ribosomal subunit but displayed no inhibitory effect on the binding of poly (U) to the ribosome. Other sulfhydryl reagents tested, like N-ethylmaleimide and iodoacetamide, did not affect the binding of Phe-tRNA to the small ribosomal subunit.", "PMID": 976437}
{"id": "pubmed23n0032_10707", "title": "Juvenile hormone analogue counteracts growth stimulation and inhibition by ecdysones in clonal Drosophila cell line.", "content": "Depending on concentration, ecdysones either stimulate or inhibit proliferation of a clonal Drosophila cell line. Both effects are counteracted by ethyl dichlorofarnesoate, a juvenile hormone analogue, which by itself is growth inhibitory. Qualitatively no difference was seen between alpha- and beta-ecdysone.", "contents": "Juvenile hormone analogue counteracts growth stimulation and inhibition by ecdysones in clonal Drosophila cell line. Depending on concentration, ecdysones either stimulate or inhibit proliferation of a clonal Drosophila cell line. Both effects are counteracted by ethyl dichlorofarnesoate, a juvenile hormone analogue, which by itself is growth inhibitory. Qualitatively no difference was seen between alpha- and beta-ecdysone.", "PMID": 976438}
{"id": "pubmed23n0032_10708", "title": "Chromosome banding patterns and the nucleolar organizer region of the eastern mole (Scalopus aquaticus).", "content": "The C and G chromosome banding patterns and the AgAS positive sites (NOR regions) of cultured lung cells of the Eastern mole (Scalopus aquaticus) are presented. A distinctive secondary constriction is found on a pair of autosomes instead of on the X-chromosome as previously believed. The presence of a heterochromatic heteromorphism is noted and a large amount of constitutive heterochromatin is present in the karyotype.", "contents": "Chromosome banding patterns and the nucleolar organizer region of the eastern mole (Scalopus aquaticus). The C and G chromosome banding patterns and the AgAS positive sites (NOR regions) of cultured lung cells of the Eastern mole (Scalopus aquaticus) are presented. A distinctive secondary constriction is found on a pair of autosomes instead of on the X-chromosome as previously believed. The presence of a heterochromatic heteromorphism is noted and a large amount of constitutive heterochromatin is present in the karyotype.", "PMID": 976439}
{"id": "pubmed23n0032_10709", "title": "Acid phosphatases in Drosophila auraria.", "content": "A study was carried out dealing with the acid phosphatases, in larvae, pupae and adults of Drosophila auraria. The most interesting finding is that the acid phosphatases are observed in 3 different phenotypes appearing as groups of 3 bands of different mobilities in the homozygotes and giving a 5 band phenotype in the heterozygote. A hypothesis is discussed in an attempt to explain the phenotypes observed and their variation during the development of the species.", "contents": "Acid phosphatases in Drosophila auraria. A study was carried out dealing with the acid phosphatases, in larvae, pupae and adults of Drosophila auraria. The most interesting finding is that the acid phosphatases are observed in 3 different phenotypes appearing as groups of 3 bands of different mobilities in the homozygotes and giving a 5 band phenotype in the heterozygote. A hypothesis is discussed in an attempt to explain the phenotypes observed and their variation during the development of the species.", "PMID": 976440}
{"id": "pubmed23n0032_10710", "title": "Direct relationship between urinary prostaglandin E and sodium excretion in essential hypertensive patients.", "content": "Urinary prostaglandins (PGEs and PGFs), sodium and potassium were measured in 17 essentially hypertensive patients. Significant positive correlations were found between a) PGEs secreted in 24 h and sodium excreted in 24 h, b) the ratio PGEs/UnaV before and PGEs/UnaV after volume expansion and c) the ration Na/K and urinary PGEs. It was suggested that renal PGEs, potent natriuretic and diuretic substances, play an important homeostatic role in the extracellular fluid regulation, and consequently in long-term control of the arterial blood pressure.", "contents": "Direct relationship between urinary prostaglandin E and sodium excretion in essential hypertensive patients. Urinary prostaglandins (PGEs and PGFs), sodium and potassium were measured in 17 essentially hypertensive patients. Significant positive correlations were found between a) PGEs secreted in 24 h and sodium excreted in 24 h, b) the ratio PGEs/UnaV before and PGEs/UnaV after volume expansion and c) the ration Na/K and urinary PGEs. It was suggested that renal PGEs, potent natriuretic and diuretic substances, play an important homeostatic role in the extracellular fluid regulation, and consequently in long-term control of the arterial blood pressure.", "PMID": 976442}
{"id": "pubmed23n0032_10711", "title": "The action of cycloheximide on the action potential and protein synthesis in medullated Xenopus axons.", "content": "Cycloheximide depresses maximum rate of change in membrane potential observed during the rising phase of the action potential in single medullated axons of Xenopus. To,e course of depression is independent of cycloheximide concentration over a range that almost completely inhibits leucine incorporation into axonal proteins.", "contents": "The action of cycloheximide on the action potential and protein synthesis in medullated Xenopus axons. Cycloheximide depresses maximum rate of change in membrane potential observed during the rising phase of the action potential in single medullated axons of Xenopus. To,e course of depression is independent of cycloheximide concentration over a range that almost completely inhibits leucine incorporation into axonal proteins.", "PMID": 976443}
{"id": "pubmed23n0032_10712", "title": "Effects of various degrees of undernutrition of mice on pregnancy and conceptus.", "content": "Maternal intake of drastically (1/2 of control) and substantially (1/2 of control) reduced feeding was studied in pregnant mice during gestation and embryonic development. Resorptions, fetal mortality rate and fetal weight were significantly affected by maternal undernutrition during the treatment period. The number of fetuses with intrauterine weight retardation was also significant.", "contents": "Effects of various degrees of undernutrition of mice on pregnancy and conceptus. Maternal intake of drastically (1/2 of control) and substantially (1/2 of control) reduced feeding was studied in pregnant mice during gestation and embryonic development. Resorptions, fetal mortality rate and fetal weight were significantly affected by maternal undernutrition during the treatment period. The number of fetuses with intrauterine weight retardation was also significant.", "PMID": 976445}
{"id": "pubmed23n0032_10713", "title": "The change of vagal activity evoked by spinal cord thermal stimulation in anesthetized rabbits.", "content": "Vagal activity decreased significantly during spinal cord warming and increased significantly during spinal cord cooling in anesthetized, immobilized rabbits. The results provide the first direct proff of changes in parsympathetic activity during spinal thermal stimulation.", "contents": "The change of vagal activity evoked by spinal cord thermal stimulation in anesthetized rabbits. Vagal activity decreased significantly during spinal cord warming and increased significantly during spinal cord cooling in anesthetized, immobilized rabbits. The results provide the first direct proff of changes in parsympathetic activity during spinal thermal stimulation.", "PMID": 976447}
{"id": "pubmed23n0032_10714", "title": "Transmural pressure as a determinant of basic intrinsic heart rate.", "content": "It was observed that the heart rate was minimum at zero transmural pressure. The mean heart rate at zero transmural pressure was 23+/-5/min. This mean heart rate increased from 23+/-5/min to a peak value of 40+/-6/min (74% acceleration) when the transmural pressure was raised from 0 to +4 mm Hg and to a similar peak value of 36+/-8/min (56% acceleration) when the transmural pressure was lowered from 0 to -4 mm Hg. The peak values attained at +/-4 mm Hg were highly significant (p less than 0.001). It is concluded that the heart rate at zero transmural pressure represents the basic intrinsic pacemaker frequency independent of neural, humoral, thermal and haemodynamically induced mechanical influences.", "contents": "Transmural pressure as a determinant of basic intrinsic heart rate. It was observed that the heart rate was minimum at zero transmural pressure. The mean heart rate at zero transmural pressure was 23+/-5/min. This mean heart rate increased from 23+/-5/min to a peak value of 40+/-6/min (74% acceleration) when the transmural pressure was raised from 0 to +4 mm Hg and to a similar peak value of 36+/-8/min (56% acceleration) when the transmural pressure was lowered from 0 to -4 mm Hg. The peak values attained at +/-4 mm Hg were highly significant (p less than 0.001). It is concluded that the heart rate at zero transmural pressure represents the basic intrinsic pacemaker frequency independent of neural, humoral, thermal and haemodynamically induced mechanical influences.", "PMID": 976448}
{"id": "pubmed23n0032_10715", "title": "Rudiments of an ability for time measurement in the cavernicolous fish Anoptichthys jordani hubbs and innes (Pisces Characidae).", "content": "Rudiments of an ability for endogenous time-measuring are indicated a) by bimodal activity in the dark phase of LD-cycles of 16:16 or 24:24 h and b) by damped activity oscillations frequently following a transition from LD to constant conditions. These oscillations always have the same period length as the applied LD.", "contents": "Rudiments of an ability for time measurement in the cavernicolous fish Anoptichthys jordani hubbs and innes (Pisces Characidae). Rudiments of an ability for endogenous time-measuring are indicated a) by bimodal activity in the dark phase of LD-cycles of 16:16 or 24:24 h and b) by damped activity oscillations frequently following a transition from LD to constant conditions. These oscillations always have the same period length as the applied LD.", "PMID": 976449}
{"id": "pubmed23n0032_10716", "title": "Distribution of H3-dimetacrine in rat cerebral cortex by electron microscopic autoradiography.", "content": "The cellular distribution of H3-dimetacrine in rat cerebral cortex was studied by electron microscopic autoradiography. A considerable proportion of autoradiographic grains (40.6%) was located over the synaptic areas, while the other strictires (i.e., dendrites, axons, glial and neuronal cells) contained less autoradiographic activity.", "contents": "Distribution of H3-dimetacrine in rat cerebral cortex by electron microscopic autoradiography. The cellular distribution of H3-dimetacrine in rat cerebral cortex was studied by electron microscopic autoradiography. A considerable proportion of autoradiographic grains (40.6%) was located over the synaptic areas, while the other strictires (i.e., dendrites, axons, glial and neuronal cells) contained less autoradiographic activity.", "PMID": 976450}
{"id": "pubmed23n0032_10717", "title": "The role of calcium in the negative inotropic effect of lanthanum.", "content": "Lanthanum reduces the rate of calcium fast exchanging processes, whereas the slowly exchanging phase is not modified. Experimental data also confirm the importance of calcium fast exchanging fraction in regulating contractile force of cardiac muscle.", "contents": "The role of calcium in the negative inotropic effect of lanthanum. Lanthanum reduces the rate of calcium fast exchanging processes, whereas the slowly exchanging phase is not modified. Experimental data also confirm the importance of calcium fast exchanging fraction in regulating contractile force of cardiac muscle.", "PMID": 976451}
{"id": "pubmed23n0032_10718", "title": "Lipoperoxide formation in the retina in ocular siderosis.", "content": "Insertion of iron nail into the vitreous cavity provoke the formation of lipoperoxide in the retina. In acord with the increase in lipoperoxide in the retina, ERG began to decrease. In vitro experiment using isolated retina, lipoperoxide was found to be increased in the presence of ferric or ferrous ions, while it was inhibited by adding antioxidants or ethylenediamine tetraacetate. From these results, direct cause of retinal degeneration in siderosis could be ascribed to the formation of lipoperosice by iron-ions liberated from the piece of iron, resulting into the degeneration of the visual cell layers of the retina.", "contents": "Lipoperoxide formation in the retina in ocular siderosis. Insertion of iron nail into the vitreous cavity provoke the formation of lipoperoxide in the retina. In acord with the increase in lipoperoxide in the retina, ERG began to decrease. In vitro experiment using isolated retina, lipoperoxide was found to be increased in the presence of ferric or ferrous ions, while it was inhibited by adding antioxidants or ethylenediamine tetraacetate. From these results, direct cause of retinal degeneration in siderosis could be ascribed to the formation of lipoperosice by iron-ions liberated from the piece of iron, resulting into the degeneration of the visual cell layers of the retina.", "PMID": 976453}
{"id": "pubmed23n0032_10719", "title": "The subcommissural organ of the lizard Lacerta s. sicula Raf. ultrastructure during the winter.", "content": "The secretory activity of the SCO cells of Lacerta s. sicula Raf. is strongly reduced during the winter. Such reduction is documented by the decrease of the decrease of the number of secretory granules type A and B described in previous papers in the summer SCO cells. Also the sacks of RER filled with electron-dense material (type C secretion) are very few; in their place there are, in the basal region of the cells, large vacuoles. In the distal region of the cells, at the free cell surface, a pronounced increase in the number of microvilli is noticed.", "contents": "The subcommissural organ of the lizard Lacerta s. sicula Raf. ultrastructure during the winter. The secretory activity of the SCO cells of Lacerta s. sicula Raf. is strongly reduced during the winter. Such reduction is documented by the decrease of the decrease of the number of secretory granules type A and B described in previous papers in the summer SCO cells. Also the sacks of RER filled with electron-dense material (type C secretion) are very few; in their place there are, in the basal region of the cells, large vacuoles. In the distal region of the cells, at the free cell surface, a pronounced increase in the number of microvilli is noticed.", "PMID": 976454}
{"id": "pubmed23n0032_10720", "title": "On the mechanism of water uptake by the developing eggs of Calotes versicolor.", "content": "Experiments show that osmotic gradient has a role in the absorption of water by the semi-cleidoic egg of Calotes versicolor. The observations also indicate that in nature the eggs need to be laid in water-saturated soil and can easily withstand flooding but not dryness.", "contents": "On the mechanism of water uptake by the developing eggs of Calotes versicolor. Experiments show that osmotic gradient has a role in the absorption of water by the semi-cleidoic egg of Calotes versicolor. The observations also indicate that in nature the eggs need to be laid in water-saturated soil and can easily withstand flooding but not dryness.", "PMID": 976455}
{"id": "pubmed23n0032_10721", "title": "[Germinal population of gonads in some chimerical embryos obtained by connecting pieces of japanese quail and domestic chick blastoderms (author's transl)].", "content": "The experimental realization of chemerical embryos (Martin's13 technic) permits a quantitative appreciation of the modalities of the colonization of quail gonads by chick germ cells. Results clearly show that nature and origin of the somatic part of the gonad areas settle the characters of the genital ridges populating, and specially the specific index of asymmetry expressed by the percentage of PGC colonizing the right gonad.", "contents": "[Germinal population of gonads in some chimerical embryos obtained by connecting pieces of japanese quail and domestic chick blastoderms (author's transl)]. The experimental realization of chemerical embryos (Martin's13 technic) permits a quantitative appreciation of the modalities of the colonization of quail gonads by chick germ cells. Results clearly show that nature and origin of the somatic part of the gonad areas settle the characters of the genital ridges populating, and specially the specific index of asymmetry expressed by the percentage of PGC colonizing the right gonad.", "PMID": 976456}
{"id": "pubmed23n0032_10722", "title": "Origin of ovarian follicle cells in birds.", "content": "In the embryonic Japanese quail ovary, transplanted on chicken chorioallantoic membrane (CAM), follicle cells are derived from somatic cells of the ovarian surface epithelium. No evidence was found for a contribution of other cell groups of the quail ovary in the formation of follicle cells. This may be demonstrated on PAS stained sections, by following the transfer of carbon particles, initially applied on the surface epithelium.", "contents": "Origin of ovarian follicle cells in birds. In the embryonic Japanese quail ovary, transplanted on chicken chorioallantoic membrane (CAM), follicle cells are derived from somatic cells of the ovarian surface epithelium. No evidence was found for a contribution of other cell groups of the quail ovary in the formation of follicle cells. This may be demonstrated on PAS stained sections, by following the transfer of carbon particles, initially applied on the surface epithelium.", "PMID": 976457}
{"id": "pubmed23n0032_10723", "title": "Association of mastopoiesis with haemopoietic tissues in the neonatal rat.", "content": "Mast cells in the newborn rat occur in harmopoietic foci of liver and spleen, but disappear from those foci as extramedullary harmopoiesis ceases during the initial postnatal month. At the same time, mast cells increasingly populate bone marrow and connective tissues of heart, lung, stomach and portal tract of liver.", "contents": "Association of mastopoiesis with haemopoietic tissues in the neonatal rat. Mast cells in the newborn rat occur in harmopoietic foci of liver and spleen, but disappear from those foci as extramedullary harmopoiesis ceases during the initial postnatal month. At the same time, mast cells increasingly populate bone marrow and connective tissues of heart, lung, stomach and portal tract of liver.", "PMID": 976458}
{"id": "pubmed23n0032_10724", "title": "Blood plasma investigations by resonance Raman spectroscopy: detection of carotenoid pigments.", "content": "Using Raman spectroscopy, we demonstrate that low levels of beta-carotene, lycopene, and xanthophyll give rise to resonance enhanced bands in blood plasma. These results explain the significance of previously unidentified spectral maxima which have been related to the state of health of the blood donor.", "contents": "Blood plasma investigations by resonance Raman spectroscopy: detection of carotenoid pigments. Using Raman spectroscopy, we demonstrate that low levels of beta-carotene, lycopene, and xanthophyll give rise to resonance enhanced bands in blood plasma. These results explain the significance of previously unidentified spectral maxima which have been related to the state of health of the blood donor.", "PMID": 976462}
{"id": "pubmed23n0032_10725", "title": "Limitations of the direct linear plot in evaluation of drug-protein binding parameters.", "content": "The applicability of the direct linear plot is compared with that of the Scatchard plot for the estimation of protein binding parameters. Only, if one class of binding sites exists in the system tested, binding parameters may be estimated by use of the direct linear plot. On the other hand the Scatchard plot also provides estimates in systems with more that one class of binding sites.", "contents": "Limitations of the direct linear plot in evaluation of drug-protein binding parameters. The applicability of the direct linear plot is compared with that of the Scatchard plot for the estimation of protein binding parameters. Only, if one class of binding sites exists in the system tested, binding parameters may be estimated by use of the direct linear plot. On the other hand the Scatchard plot also provides estimates in systems with more that one class of binding sites.", "PMID": 976464}
{"id": "pubmed23n0032_10726", "title": "Funding for reproductive research: the status and the needs.", "content": "Just to maintain the admittedly inadequate level of research in reproduction and contraceptive development attained in 1974 would require an investment of $143 million this year--a level that has not been reached. To take advantage of existing knowledge, at least $361 million would be required--increasing to $498 million by 1980. To achieve a high-priority research program--10 percent of health expenditures--would require $500 million this year--rising to $766 million by 1980.", "contents": "Funding for reproductive research: the status and the needs. Just to maintain the admittedly inadequate level of research in reproduction and contraceptive development attained in 1974 would require an investment of $143 million this year--a level that has not been reached. To take advantage of existing knowledge, at least $361 million would be required--increasing to $498 million by 1980. To achieve a high-priority research program--10 percent of health expenditures--would require $500 million this year--rising to $766 million by 1980.", "PMID": 976465}
{"id": "pubmed23n0032_10727", "title": "How old is too old? Age and the sociology of fertility.", "content": "Are we due for a new baby boom as couples who had postponed childbearing begin to make up for lost time? Analysis of National Fertility Study data suggest that \"later means fewer\"--quite apart from the declining fecundity that occurs at older ages. Couples who delay having children until relatively late in life are subject to fewer pressures to have children (or more children) even as the reasons not to begin (or increase) their families become more salient.", "contents": "How old is too old? Age and the sociology of fertility. Are we due for a new baby boom as couples who had postponed childbearing begin to make up for lost time? Analysis of National Fertility Study data suggest that \"later means fewer\"--quite apart from the declining fecundity that occurs at older ages. Couples who delay having children until relatively late in life are subject to fewer pressures to have children (or more children) even as the reasons not to begin (or increase) their families become more salient.", "PMID": 976466}
{"id": "pubmed23n0032_10728", "title": "Family planning needs and services in nonmetropolitan areas.", "content": "More than 215 million low- and marginal-income women living in rural areas are still without access to family planning services they want and need. Priority attention to more populous areas where there are no organized programs, or just one agency providing services, is suggested. Increased involvement of hospitals is necessary if the rural-urban gap is to be closed.", "contents": "Family planning needs and services in nonmetropolitan areas. More than 215 million low- and marginal-income women living in rural areas are still without access to family planning services they want and need. Priority attention to more populous areas where there are no organized programs, or just one agency providing services, is suggested. Increased involvement of hospitals is necessary if the rural-urban gap is to be closed.", "PMID": 976467}
{"id": "pubmed23n0032_10729", "title": "The U.S. fertility decline, 1961-1975: the contribution of changes in marital status and marital fertility.", "content": "Changes in marital fertility have accounted for 83 percent of the decline in total fertility since 1971; changes in marital status accounted for 19 percent, and changes in nonmarital fertility had only a negligible effect. Declines in the level of marital fertility account for a substantial part of the overall decrease; postponement of childbearing did not occur in any significant degree until the 1970s.", "contents": "The U.S. fertility decline, 1961-1975: the contribution of changes in marital status and marital fertility. Changes in marital fertility have accounted for 83 percent of the decline in total fertility since 1971; changes in marital status accounted for 19 percent, and changes in nonmarital fertility had only a negligible effect. Declines in the level of marital fertility account for a substantial part of the overall decrease; postponement of childbearing did not occur in any significant degree until the 1970s.", "PMID": 976468}
{"id": "pubmed23n0032_10730", "title": "Quantitative studies of the pathway to acute carrageenan inflammation.", "content": "A 12-step scheme representing initiation and development of acute carrageenan inflammation in the rat has been devised. Simultaneous quantitative temporal measurement of the number of inflammatory cells mobilized and edema volume produced as carrageenan pleurisy developed helped elucidate several of the early steps, In the pleurisy a 20-min lag phase preceded both the mobilization of neutrophils and edema formation. Temporal histological studies suggested that the lag phase represents the time needed for the neutrophil chemotactic factor to be generated and/or released (steps 2 and 3) in addition to the time required for these cells to move through the walls of the capillaries into the pleural space (steps 4 and 5). The neutrophil chemotactic factor is unknown. The complement system is not involved since cobra venom factor-treated animals produced a normal edematous and cellular response to carrageenan in spite of severely depressed complement levels. The mobilized neutrophils are believed to phagocytize the carrageenan(step6). In this process lysosomal enzymes are released (step 7). Drug inhibition studies suggest that lysosomal enzymes are not the edemagenic agents in carrageenan inflammation. In the scheme these enzymes are responsible for activation of the prostaglandin biosynthetic chain (step 8). Prostaglandin biosynthesis (step 9) leads to the release of an intermediate (step 10), as yet unidentified, which is responsible for increased tissue permeability (step 11) and edema formation (step 12). Histamine, serotonin, bradykinin, arachidonic acid, and prostaglandin's E1 and E2 are not deemagenic in the rat pleural cavity and therefore cannot be responsible for edema formation. Aspirin and indomethacin reduce the edema produced by carrageenan in a dose-related manner without affecting the magnitude or the time course of neutrophil mobilization. These findings led to the concept that edema formation is not the result of inflammatory cell mobilization but is rather a consequence of cellular activity, presumably phagocytosis, after mobilization.", "contents": "Quantitative studies of the pathway to acute carrageenan inflammation. A 12-step scheme representing initiation and development of acute carrageenan inflammation in the rat has been devised. Simultaneous quantitative temporal measurement of the number of inflammatory cells mobilized and edema volume produced as carrageenan pleurisy developed helped elucidate several of the early steps, In the pleurisy a 20-min lag phase preceded both the mobilization of neutrophils and edema formation. Temporal histological studies suggested that the lag phase represents the time needed for the neutrophil chemotactic factor to be generated and/or released (steps 2 and 3) in addition to the time required for these cells to move through the walls of the capillaries into the pleural space (steps 4 and 5). The neutrophil chemotactic factor is unknown. The complement system is not involved since cobra venom factor-treated animals produced a normal edematous and cellular response to carrageenan in spite of severely depressed complement levels. The mobilized neutrophils are believed to phagocytize the carrageenan(step6). In this process lysosomal enzymes are released (step 7). Drug inhibition studies suggest that lysosomal enzymes are not the edemagenic agents in carrageenan inflammation. In the scheme these enzymes are responsible for activation of the prostaglandin biosynthetic chain (step 8). Prostaglandin biosynthesis (step 9) leads to the release of an intermediate (step 10), as yet unidentified, which is responsible for increased tissue permeability (step 11) and edema formation (step 12). Histamine, serotonin, bradykinin, arachidonic acid, and prostaglandin's E1 and E2 are not deemagenic in the rat pleural cavity and therefore cannot be responsible for edema formation. Aspirin and indomethacin reduce the edema produced by carrageenan in a dose-related manner without affecting the magnitude or the time course of neutrophil mobilization. These findings led to the concept that edema formation is not the result of inflammatory cell mobilization but is rather a consequence of cellular activity, presumably phagocytosis, after mobilization.", "PMID": 976489}
{"id": "pubmed23n0032_10731", "title": "The effect of certain vitamin deficiencies on hepatic drug metabolism.", "content": "There is increasing evidence that the liver microsomal drug metabolizing system is affected by various vitamins such as ascorbic acid, riboflavin, and alpha-tocopherol. In regard to ascorbic acid deficiency there is a decrease in the quantity of hepatic microsomal electron transport components such as cytochrome P-450 and NADPH-cytochrome P-450 reductase, as well as decreases in a variety of drug enzyme reactions such as N-demethylation, O-demethylation, and steroid hydroxylation. In addition, young animals given high supplements of vitamin C have increased quantities of electron transport components and overall drug metabolism activities. Kinetic studies indicate no change in the apparent Km of N-demethylase, O-demethylase or hydroxylase for drug substrates in animals depleted or given high amounts of the vitamin. However, there are qualitative changes in both type I and II substrate-cytochrome P-450 binding. Ascorbic acid is not involved in microsomal lipid peroxidation or in any qualitative or quantitative change in phosphatidylcholine. Replenishing vitamin C-deficient animals with ascorbic acid required 3 to 7 days for the electron transport components and drug metabolism activities to return to normal levels. Induction with phenobarbital and 3-methylcholanthrene is not impaired in the deficient animal since drug metabolism activities are induced to the same extent as normal controls; however, the administration of delta-aminolevulinic acid, a precursor of heme synthesis, to deficient animals caused an increase in the quantity of cytochrome P-450. The effects of riboflavin deficiency on electron transport components and drug metabolism activities have been noted only in adult animals after prolonged periods of deficiency. Decreases in drug metabolism activities occur with both type I (aminopyrine and ethylmorphine) and type II (aniline) substrates. As was found with ascorbic acid deficiency, drug enzyme induction occurred to the same extent with phenobarbital in deficient and normal animals. In addition, it required from 10 to 15 days for the drug metabolism activities to return to normal levels when deficient animals were replenished with riboflavin. The effect of vitamin E on drug metabolism is specific in N-demethylase activities decrease while O-demethylase activities are not affected in the deficient state. This vitamin differs from ascorbic acid and riboflavin in that several laboratories have reported no quantitative decrease in cytochrome P-450, although there are some reports that it and delta-aminolevulinic acid dehydratase are lowered quantity of cytochrome in E-deficient animals. The effect of vitamin E, if any, on the P-450 is unresolved; an important question that requires further clarification. As with ascorbic acid there is no difference in the apparent Km of N-demethylase enzymes for varous substrates and the protective effect of vitamin E does not appear to be one of an antioxidant inhibiting microsomal lipid peroxidation.", "contents": "The effect of certain vitamin deficiencies on hepatic drug metabolism. There is increasing evidence that the liver microsomal drug metabolizing system is affected by various vitamins such as ascorbic acid, riboflavin, and alpha-tocopherol. In regard to ascorbic acid deficiency there is a decrease in the quantity of hepatic microsomal electron transport components such as cytochrome P-450 and NADPH-cytochrome P-450 reductase, as well as decreases in a variety of drug enzyme reactions such as N-demethylation, O-demethylation, and steroid hydroxylation. In addition, young animals given high supplements of vitamin C have increased quantities of electron transport components and overall drug metabolism activities. Kinetic studies indicate no change in the apparent Km of N-demethylase, O-demethylase or hydroxylase for drug substrates in animals depleted or given high amounts of the vitamin. However, there are qualitative changes in both type I and II substrate-cytochrome P-450 binding. Ascorbic acid is not involved in microsomal lipid peroxidation or in any qualitative or quantitative change in phosphatidylcholine. Replenishing vitamin C-deficient animals with ascorbic acid required 3 to 7 days for the electron transport components and drug metabolism activities to return to normal levels. Induction with phenobarbital and 3-methylcholanthrene is not impaired in the deficient animal since drug metabolism activities are induced to the same extent as normal controls; however, the administration of delta-aminolevulinic acid, a precursor of heme synthesis, to deficient animals caused an increase in the quantity of cytochrome P-450. The effects of riboflavin deficiency on electron transport components and drug metabolism activities have been noted only in adult animals after prolonged periods of deficiency. Decreases in drug metabolism activities occur with both type I (aminopyrine and ethylmorphine) and type II (aniline) substrates. As was found with ascorbic acid deficiency, drug enzyme induction occurred to the same extent with phenobarbital in deficient and normal animals. In addition, it required from 10 to 15 days for the drug metabolism activities to return to normal levels when deficient animals were replenished with riboflavin. The effect of vitamin E on drug metabolism is specific in N-demethylase activities decrease while O-demethylase activities are not affected in the deficient state. This vitamin differs from ascorbic acid and riboflavin in that several laboratories have reported no quantitative decrease in cytochrome P-450, although there are some reports that it and delta-aminolevulinic acid dehydratase are lowered quantity of cytochrome in E-deficient animals. The effect of vitamin E, if any, on the P-450 is unresolved; an important question that requires further clarification. As with ascorbic acid there is no difference in the apparent Km of N-demethylase enzymes for varous substrates and the protective effect of vitamin E does not appear to be one of an antioxidant inhibiting microsomal lipid peroxidation.", "PMID": 976490}
{"id": "pubmed23n0032_10732", "title": "The effect of quantity and quality of dietary protein on drug metabolism.", "content": "Dietary protein deficiency is known to modify the response to the pharmacotoxicological activities of drugs and foreign compounds, due in part to altered rates of metabolism. Prediction of whether in vivo susceptibilities to foreign compounds are increased or decreased in protein deficient animals has been said to be related to the relative toxicites of the metabolic products. We have shown that weanling rats fed semipurified casein diets for 15 days show a 75% depression of hepatic microsomal mixed function oxidase activities. About one-fourth of this decrease is due to a retardation of the normal rate of liver cell proliferation and less microsomal protein; the remaining three-fourths is due to a reduction of the specific enzyme activity. This latter decrease is closely correlated with similar decreases in cytochrome P-450 and cytochrome c reductase activities and cytochrome P-450 contents. Although protein deficiency affects the relative contents of phosphatidylcholine and cytochrome P-450, this does not result in modifications of the Km for metabolism, as is seen with phenobarbital administration in the various dietary groups. The depression of mixed function oxidase enzyme activities caused by feeding the protein deficient diet for 15 days can be restored to normal by feeding the 20% casein diets for an additional 30 days in the case of aniline hydroxylase but only partially in the case of ethylmorphine N-demethylase. The complexities of determining the role of metabolism as a modulator of protein deficiency effects on foreign compound toxicity are discussed.", "contents": "The effect of quantity and quality of dietary protein on drug metabolism. Dietary protein deficiency is known to modify the response to the pharmacotoxicological activities of drugs and foreign compounds, due in part to altered rates of metabolism. Prediction of whether in vivo susceptibilities to foreign compounds are increased or decreased in protein deficient animals has been said to be related to the relative toxicites of the metabolic products. We have shown that weanling rats fed semipurified casein diets for 15 days show a 75% depression of hepatic microsomal mixed function oxidase activities. About one-fourth of this decrease is due to a retardation of the normal rate of liver cell proliferation and less microsomal protein; the remaining three-fourths is due to a reduction of the specific enzyme activity. This latter decrease is closely correlated with similar decreases in cytochrome P-450 and cytochrome c reductase activities and cytochrome P-450 contents. Although protein deficiency affects the relative contents of phosphatidylcholine and cytochrome P-450, this does not result in modifications of the Km for metabolism, as is seen with phenobarbital administration in the various dietary groups. The depression of mixed function oxidase enzyme activities caused by feeding the protein deficient diet for 15 days can be restored to normal by feeding the 20% casein diets for an additional 30 days in the case of aniline hydroxylase but only partially in the case of ethylmorphine N-demethylase. The complexities of determining the role of metabolism as a modulator of protein deficiency effects on foreign compound toxicity are discussed.", "PMID": 976491}
{"id": "pubmed23n0032_10733", "title": "Angiotensin antagonists and the adrenal cortex and medulla.", "content": "Several analogs of angiotensin in which the phenylalanine in position 8 of the peptide chain was replaced by an aliphatic amino acid residue are specific antagonists of angiotensin in aorta, the adrenal medulla, and adrenal zona glomerulosa. In the adrenal cortex and medulla, all actapeptide analogs have more agonist activity than in aortic strips. In studies with N-terminally substituted analogs, it appears that adrenal degradation of the angiotensin molecule by aminopeptidase(s) does not occur or is not retarded by N-terminal mocifications such as sarcosine substitution. The decapeptide analog [Ile8]-angiotensin I and heptapeptide analog [des-Asp1, Ile8]-angiotensin II were excellent antagonists in the adrenal medulla and each peptide was devoid of intrinsic activity. These substituted homologs of angiotensin may offer a novel approach for the development of selective antagonists of angiotensin receptors. In the adrenal cotex, [des-Asp1, Ile8]-heptapeptide possessed greater receptor affinity than any of the angiotensin octapeptides studied. This C-terminally substituted heptapeptide does have significant intrinsic activity in the adrenal cortex which would limit the use of this compound as an antagonist of vascular responses to angiotensin II. In studies with [Ile8]-angiotensin II, [Sar1, Ile8]-angiotensin II, and [des-Asp1, Ile8]-angiotensin II, the pA2 values calculated indicate that the N-terminal residue is not important for receptor binding in the adrenal cortex but may be of significance in binding to adrenal medullary and aortic smooth muscle receptors. At the present time it appears unlikely that any single animal model or assay system can reliably predict the agoinst/antagonist activities of angiotensin analogs for all the various end organs which respond to the angiotensins.", "contents": "Angiotensin antagonists and the adrenal cortex and medulla. Several analogs of angiotensin in which the phenylalanine in position 8 of the peptide chain was replaced by an aliphatic amino acid residue are specific antagonists of angiotensin in aorta, the adrenal medulla, and adrenal zona glomerulosa. In the adrenal cortex and medulla, all actapeptide analogs have more agonist activity than in aortic strips. In studies with N-terminally substituted analogs, it appears that adrenal degradation of the angiotensin molecule by aminopeptidase(s) does not occur or is not retarded by N-terminal mocifications such as sarcosine substitution. The decapeptide analog [Ile8]-angiotensin I and heptapeptide analog [des-Asp1, Ile8]-angiotensin II were excellent antagonists in the adrenal medulla and each peptide was devoid of intrinsic activity. These substituted homologs of angiotensin may offer a novel approach for the development of selective antagonists of angiotensin receptors. In the adrenal cotex, [des-Asp1, Ile8]-heptapeptide possessed greater receptor affinity than any of the angiotensin octapeptides studied. This C-terminally substituted heptapeptide does have significant intrinsic activity in the adrenal cortex which would limit the use of this compound as an antagonist of vascular responses to angiotensin II. In studies with [Ile8]-angiotensin II, [Sar1, Ile8]-angiotensin II, and [des-Asp1, Ile8]-angiotensin II, the pA2 values calculated indicate that the N-terminal residue is not important for receptor binding in the adrenal cortex but may be of significance in binding to adrenal medullary and aortic smooth muscle receptors. At the present time it appears unlikely that any single animal model or assay system can reliably predict the agoinst/antagonist activities of angiotensin analogs for all the various end organs which respond to the angiotensins.", "PMID": 976492}
{"id": "pubmed23n0032_10734", "title": "The use of angiotensin II blockade to study adrenal steroid secretion.", "content": "In the dog, the renin--angiotensin system appears to be a primary control mechanism for aldosterone secretion since angiotensin II blockade decreased aldosterone production to undetectable levels. Angiotensin II blockade also decreased cortisol secretion strikingly in dogs with thoracic caval constriction, a finding which suggests the presence of an angiotensin II receptor in the two inner zones of adrenal cortex. An important incidental finding after angiotensin II blockade in both sodium-depleted dogs and dogs with thoracic caval constriction was the striking drop in arterial pressure. It is suggested that angiotensin II acts on the peripheral arterioles to provide an important compensatory mechanism and, thereby, maintain arterial pressure in these low cardiac output states. In the rat, both the nonapeptide converting enzyme inhibitor and [Sar1, Ala8]-angiotensin II produced a marked decrease in aldosterone secretion in hypophysectomized, sodium-depleted animals. Both synthetic angiotensin II and its heptapeptide fragment produced striking increases in aldosterone secretion when the obscuring effect of ACTH was excluded in the rat. These findings provide evidence that the renin--angiotensin system is an important control mechanism for aldosterone biosynthesis in the rat.", "contents": "The use of angiotensin II blockade to study adrenal steroid secretion. In the dog, the renin--angiotensin system appears to be a primary control mechanism for aldosterone secretion since angiotensin II blockade decreased aldosterone production to undetectable levels. Angiotensin II blockade also decreased cortisol secretion strikingly in dogs with thoracic caval constriction, a finding which suggests the presence of an angiotensin II receptor in the two inner zones of adrenal cortex. An important incidental finding after angiotensin II blockade in both sodium-depleted dogs and dogs with thoracic caval constriction was the striking drop in arterial pressure. It is suggested that angiotensin II acts on the peripheral arterioles to provide an important compensatory mechanism and, thereby, maintain arterial pressure in these low cardiac output states. In the rat, both the nonapeptide converting enzyme inhibitor and [Sar1, Ala8]-angiotensin II produced a marked decrease in aldosterone secretion in hypophysectomized, sodium-depleted animals. Both synthetic angiotensin II and its heptapeptide fragment produced striking increases in aldosterone secretion when the obscuring effect of ACTH was excluded in the rat. These findings provide evidence that the renin--angiotensin system is an important control mechanism for aldosterone biosynthesis in the rat.", "PMID": 976493}
{"id": "pubmed23n0032_10735", "title": "Clinical pharmacology of angiotensin antagonists.", "content": "The early clinical pharmacologic investigations of saralasin were facilitated by the availability of a highly sensitive and specific radioimmunoassay for this peptide. In these studies, plasma concentrations of saralasin were correlated with inhibition of angiotensin receptors in each of three organ systems: vascular smooth muscle, adrenal cortex, and the renin release control mechanism in the kidney. The biochemical half-life of plasma saralasin was 3.2 min and an infusion time-to-plateau was 12--15 min. Saralasin inhibited adrenal cortical, vascular, and intrarenal (renin release) angiotensin receptors. The time required for manifesting these blocking actions was short(3--10 min) except for the 30--60 min required to suppress plasma aldosterone. Saralasin-induced blood pressure lowering was dependent on previously elevated serum renin activity and volume depletion. Expansion of intravascular volume prevented hypotensive responses to saralasin even in high-renin patients. Saralasin-induced renin release occurred independent of hypotension and could be inhibited by propranolol, a beta-adrenergic blocking agent. Thus, saralasin is a selective angiotensin antagonist which lacks organ specificity. It is a highly useful tool in pharmacologic studies of the renin--angiotensin axis in man and shows promise as a diagnostic tool.", "contents": "Clinical pharmacology of angiotensin antagonists. The early clinical pharmacologic investigations of saralasin were facilitated by the availability of a highly sensitive and specific radioimmunoassay for this peptide. In these studies, plasma concentrations of saralasin were correlated with inhibition of angiotensin receptors in each of three organ systems: vascular smooth muscle, adrenal cortex, and the renin release control mechanism in the kidney. The biochemical half-life of plasma saralasin was 3.2 min and an infusion time-to-plateau was 12--15 min. Saralasin inhibited adrenal cortical, vascular, and intrarenal (renin release) angiotensin receptors. The time required for manifesting these blocking actions was short(3--10 min) except for the 30--60 min required to suppress plasma aldosterone. Saralasin-induced blood pressure lowering was dependent on previously elevated serum renin activity and volume depletion. Expansion of intravascular volume prevented hypotensive responses to saralasin even in high-renin patients. Saralasin-induced renin release occurred independent of hypotension and could be inhibited by propranolol, a beta-adrenergic blocking agent. Thus, saralasin is a selective angiotensin antagonist which lacks organ specificity. It is a highly useful tool in pharmacologic studies of the renin--angiotensin axis in man and shows promise as a diagnostic tool.", "PMID": 976494}
{"id": "pubmed23n0032_10736", "title": "Luteinizing hormone and progesterone in women under postcoital contraception with D-norgestrel.", "content": "Luteinizing hormone (LH) and progesterone (Pg) levels in blood were measured simultaneously by radioimmunoassay during 53 menstrual cycles in order to investigate the effect of 400 mug of D-norgestrel, administered postcoitally, on pituitary and ovarian function. Of 31 control cycles, 2 appeared to be anovulatory, since the LH peak and subsequent Pg elevation were absent. Twenty-nine cycles showed typical LH surges in the middle of the cycle, followed by a manifold Pg increase. Twelve women received 5 to 13 tables of D-norgestrel. A total absence or at least marked suppression of LH and Pg elevations was observed. In a third group, D-norgestrel was administered on scheduled days. Each woman ingested one to four tablets between the 6th and 18th days of the cycle. Two or more tablets disturbed LH and Pg ovulatory patterns. Of four women who received a tablet on day 10, one failed to show characteristic ovulatory patterns and three exhibited a delay in the time of the LH and Pg increase. These results demonstrate that D-norgestrel in a postcoital regimen alters pituitary and ovarian function, strongly suggesting an antiovulatory effect.", "contents": "Luteinizing hormone and progesterone in women under postcoital contraception with D-norgestrel. Luteinizing hormone (LH) and progesterone (Pg) levels in blood were measured simultaneously by radioimmunoassay during 53 menstrual cycles in order to investigate the effect of 400 mug of D-norgestrel, administered postcoitally, on pituitary and ovarian function. Of 31 control cycles, 2 appeared to be anovulatory, since the LH peak and subsequent Pg elevation were absent. Twenty-nine cycles showed typical LH surges in the middle of the cycle, followed by a manifold Pg increase. Twelve women received 5 to 13 tables of D-norgestrel. A total absence or at least marked suppression of LH and Pg elevations was observed. In a third group, D-norgestrel was administered on scheduled days. Each woman ingested one to four tablets between the 6th and 18th days of the cycle. Two or more tablets disturbed LH and Pg ovulatory patterns. Of four women who received a tablet on day 10, one failed to show characteristic ovulatory patterns and three exhibited a delay in the time of the LH and Pg increase. These results demonstrate that D-norgestrel in a postcoital regimen alters pituitary and ovarian function, strongly suggesting an antiovulatory effect.", "PMID": 976495}
{"id": "pubmed23n0032_10737", "title": "A comparison of endometrial histology with simultaneous plasma progesterone determinations in infertile women.", "content": "A retrospective analysis of 238 endometrial biopsies and simultaneous plasma progesterone determinations performed during the course of infertility evaluations is presented. The data suggest a high (93%) correlation between the two procedures for the detection of ovulation. Endometrial abnormalities were detected in 5% of the biopsies. Shortening of the cycle was noted in 36% of the patients, while only 3% demonstrated a longer cycle. Simultaneous measurement of plasma progesterone levels in association with the endometrial biopsy is believed to better document corpus luteum defects. One should be aware of the frequent occurrence of a shortened cycle on the basis of postbiopsy bleeding. Basal body temperature graphs should be utilized prior to biopsy in patients with a history of irregular menstrual cycles.", "contents": "A comparison of endometrial histology with simultaneous plasma progesterone determinations in infertile women. A retrospective analysis of 238 endometrial biopsies and simultaneous plasma progesterone determinations performed during the course of infertility evaluations is presented. The data suggest a high (93%) correlation between the two procedures for the detection of ovulation. Endometrial abnormalities were detected in 5% of the biopsies. Shortening of the cycle was noted in 36% of the patients, while only 3% demonstrated a longer cycle. Simultaneous measurement of plasma progesterone levels in association with the endometrial biopsy is believed to better document corpus luteum defects. One should be aware of the frequent occurrence of a shortened cycle on the basis of postbiopsy bleeding. Basal body temperature graphs should be utilized prior to biopsy in patients with a history of irregular menstrual cycles.", "PMID": 976496}
{"id": "pubmed23n0032_10738", "title": "Hysteroscopic procedures in 257 patients.", "content": "Two hundred and fifty-seven hysteroscopic examinations were performed on patients who complained primarily of infertility and abnormal uterine bleeding. Organic intrauterine lesions were identified, and correlations were made with preoperative hysterograms and the tissue obtained for pathologic evaluation. The technique proved of special value for the location and resection of intrauterine devices. Failure to observe the cavity adequately and to complete the examination occurred in 20 (8%) of the patients. The most serious complication, uterine perforation, occurred in five instances but did not require additional treatment.", "contents": "Hysteroscopic procedures in 257 patients. Two hundred and fifty-seven hysteroscopic examinations were performed on patients who complained primarily of infertility and abnormal uterine bleeding. Organic intrauterine lesions were identified, and correlations were made with preoperative hysterograms and the tissue obtained for pathologic evaluation. The technique proved of special value for the location and resection of intrauterine devices. Failure to observe the cavity adequately and to complete the examination occurred in 20 (8%) of the patients. The most serious complication, uterine perforation, occurred in five instances but did not require additional treatment.", "PMID": 976497}
{"id": "pubmed23n0032_10739", "title": "Pregnancy during the hysterogram cycle.", "content": "Because of potential mutagenic effects of radiation on the soon-to-be fertilized ovum, we questioned whether we should advise against conception during the cycle in which a hysterosalpingogram is performed. Our results do not suggest any increase in the incidence of spontaneous abortion or congenital anomalies in these patients. Therefore, we see no need to advise against conception during the cycle in which a hysterosalpingogram is performed.", "contents": "Pregnancy during the hysterogram cycle. Because of potential mutagenic effects of radiation on the soon-to-be fertilized ovum, we questioned whether we should advise against conception during the cycle in which a hysterosalpingogram is performed. Our results do not suggest any increase in the incidence of spontaneous abortion or congenital anomalies in these patients. Therefore, we see no need to advise against conception during the cycle in which a hysterosalpingogram is performed.", "PMID": 976498}
{"id": "pubmed23n0032_10740", "title": "Reconstructive surgery for congenital atresia of the uterine cervix.", "content": "Reconstructive surgery for congenital atresia of the uterine cervix, utilizing the atretic cervix as a conduit for the permanent fistulous communication between the endometrial cavity and vagina, has resulted in the establishment of cyclic menses in four patients followed for up to 27 months postoperatively. A detailed review of maternal obstetric histories failed to reveal a common factor causally related to this extremely unusual and formerly hopeless congenital anomaly of the M\u00fcllerian ducts.", "contents": "Reconstructive surgery for congenital atresia of the uterine cervix. Reconstructive surgery for congenital atresia of the uterine cervix, utilizing the atretic cervix as a conduit for the permanent fistulous communication between the endometrial cavity and vagina, has resulted in the establishment of cyclic menses in four patients followed for up to 27 months postoperatively. A detailed review of maternal obstetric histories failed to reveal a common factor causally related to this extremely unusual and formerly hopeless congenital anomaly of the M\u00fcllerian ducts.", "PMID": 976499}
{"id": "pubmed23n0032_10741", "title": "Improved motility recovery of human spermatozoa after freeze preservation via a new approach.", "content": "A new sperm isolation technique was studied in conjunction with the short-term freeze preservation of human spermatozoa. The isolation procedure yielded subpopulations of spermatozoa with very high percentage motility and progressive motility score, and which were virtually free of seminal debris. For 24 semen samples from 13 donors, the mean prefreeze values of percentage motility and motility score were 53% (2.8), 73% (3.1), and 88% (3.5) for the parent semen, 7.5% BSA (middle) fractions, and 17.5% BSA (bottom) fractions, respectively. These samples were used without a priori constraint on semen quality. Eleven samples were preselected on the basis of good motility in the semen. These yielded prefreeze motility values of 66% (3.2), 70% (3.4), and 87% (3.9) for the semen and two fractions, respectively. Sperm motility in the middle fractions was thus intermediate to that in the semen and in the bottom fractions, although closer to the former in these 11 cases. The sperm freeze preservation procedure involved dilution of the semen samples and separated sperm fractions with a cryoprotective semen extender, and freezing and thawing in a conventional manner. Post-thaw percentage motility, motility score, and percentage survival were substantially higher for the separated fractions than for the parent semen. For the 24 cases, the bottom fractions yielded mean values of 57% motility, 3.0 motility score, and 70% survival, in comparison with the respective values of 20%, 2.3, and 34% for the semen. In the 11 preselected cases, the bottom fractions yielded post-thaw mean values of 60% motility, 3.3 motility score, and 72% survival; the middle fractions yielded respective values of 45%, 3.1, and 64%; and the parent semen yielded respective values of 27%, 2.3, and 41%. It was concluded that the major factor in improving post-thaw motility recovery was the separation process as a whole, rather than the degree of separation.", "contents": "Improved motility recovery of human spermatozoa after freeze preservation via a new approach. A new sperm isolation technique was studied in conjunction with the short-term freeze preservation of human spermatozoa. The isolation procedure yielded subpopulations of spermatozoa with very high percentage motility and progressive motility score, and which were virtually free of seminal debris. For 24 semen samples from 13 donors, the mean prefreeze values of percentage motility and motility score were 53% (2.8), 73% (3.1), and 88% (3.5) for the parent semen, 7.5% BSA (middle) fractions, and 17.5% BSA (bottom) fractions, respectively. These samples were used without a priori constraint on semen quality. Eleven samples were preselected on the basis of good motility in the semen. These yielded prefreeze motility values of 66% (3.2), 70% (3.4), and 87% (3.9) for the semen and two fractions, respectively. Sperm motility in the middle fractions was thus intermediate to that in the semen and in the bottom fractions, although closer to the former in these 11 cases. The sperm freeze preservation procedure involved dilution of the semen samples and separated sperm fractions with a cryoprotective semen extender, and freezing and thawing in a conventional manner. Post-thaw percentage motility, motility score, and percentage survival were substantially higher for the separated fractions than for the parent semen. For the 24 cases, the bottom fractions yielded mean values of 57% motility, 3.0 motility score, and 70% survival, in comparison with the respective values of 20%, 2.3, and 34% for the semen. In the 11 preselected cases, the bottom fractions yielded post-thaw mean values of 60% motility, 3.3 motility score, and 72% survival; the middle fractions yielded respective values of 45%, 3.1, and 64%; and the parent semen yielded respective values of 27%, 2.3, and 41%. It was concluded that the major factor in improving post-thaw motility recovery was the separation process as a whole, rather than the degree of separation.", "PMID": 976500}
{"id": "pubmed23n0032_10742", "title": "Effect of prolactin on metabolism of human spermatozoa.", "content": "The effect of prolactin on adenyl cyclase, rate of fructose utilization, and glucose oxidation by human spermatozoa was studied. Prolactin stimulated all of these processes at a concentration generally available in seminal plasma. These results suggest that prolactin plays an important role in the energy metabolism of human spermatozoa.", "contents": "Effect of prolactin on metabolism of human spermatozoa. The effect of prolactin on adenyl cyclase, rate of fructose utilization, and glucose oxidation by human spermatozoa was studied. Prolactin stimulated all of these processes at a concentration generally available in seminal plasma. These results suggest that prolactin plays an important role in the energy metabolism of human spermatozoa.", "PMID": 976501}
{"id": "pubmed23n0032_10743", "title": "Inhibition of the oxidative metabolism of human spermatozoa by a heat-labile factor in seminal plasma.", "content": "The effect of different media on the oxidative and glycolytic metabolism and on the motility of human spermatozoa has been investigated. Sperm resuspended in Ringer's solution produce more carbon dioxide than sperm in the original seminal plasma. In addition, sperm resuspended in heated seminal plasma have a higher carbon dioxide production and higher oxygen uptake than sperm in untreated seminal plasma. The mean lactic acid production was not significantly affected by different media. The motility of sperm in heated seminal plasma was poorer than that in untreated seminal plasma, although the percentage of cells that stained with eosin did not differ in the two media. After being heated, seminal plasma not only continued to take up oxygen but did so at a higher rate. The results of the present study suggest that seminal plasma contains a heat-labile component that inhibits the oxidative metabolism of human sperm but does not significantly alter sperm glycolysis.", "contents": "Inhibition of the oxidative metabolism of human spermatozoa by a heat-labile factor in seminal plasma. The effect of different media on the oxidative and glycolytic metabolism and on the motility of human spermatozoa has been investigated. Sperm resuspended in Ringer's solution produce more carbon dioxide than sperm in the original seminal plasma. In addition, sperm resuspended in heated seminal plasma have a higher carbon dioxide production and higher oxygen uptake than sperm in untreated seminal plasma. The mean lactic acid production was not significantly affected by different media. The motility of sperm in heated seminal plasma was poorer than that in untreated seminal plasma, although the percentage of cells that stained with eosin did not differ in the two media. After being heated, seminal plasma not only continued to take up oxygen but did so at a higher rate. The results of the present study suggest that seminal plasma contains a heat-labile component that inhibits the oxidative metabolism of human sperm but does not significantly alter sperm glycolysis.", "PMID": 976502}
{"id": "pubmed23n0032_10744", "title": "Relationship between motility and the transport and binding of divalent cations to the plasma membrane of human spermatozoa.", "content": "Measurements of fluorescence changes of the divalent cation probe chlortetracycline, which accumulates in the plasma membrane of human spermatozoa, and measurements of radioactive calcium uptake indicate that the plasma membrane of human spermatozoa is impermeable to calcium. Moreover, permeability to exogenous calcium is not changed when sperm motility is stimulated by agents which increase intracellular levels of adenosine 3':5'-monophosphate (cyclic AMP). Evidence is presented, however, which suggests that energy-dependent divalent cation binding to the plasma membrane is lowered when motility is increased by cyclic AMP phosphodiesterase inhibitors.", "contents": "Relationship between motility and the transport and binding of divalent cations to the plasma membrane of human spermatozoa. Measurements of fluorescence changes of the divalent cation probe chlortetracycline, which accumulates in the plasma membrane of human spermatozoa, and measurements of radioactive calcium uptake indicate that the plasma membrane of human spermatozoa is impermeable to calcium. Moreover, permeability to exogenous calcium is not changed when sperm motility is stimulated by agents which increase intracellular levels of adenosine 3':5'-monophosphate (cyclic AMP). Evidence is presented, however, which suggests that energy-dependent divalent cation binding to the plasma membrane is lowered when motility is increased by cyclic AMP phosphodiesterase inhibitors.", "PMID": 976503}
{"id": "pubmed23n0032_10745", "title": "Temporal relationships critical to estrogen-induced delay of ovum transport.", "content": "Previous studies have shown that a pharmacologic dose of estradiol given at the time of injection of human chorionic gonadotropin (HCG) significantly delays the transport of ova through the rabbit oviduct and that the site of delay is located at the ampullary-isthmic junction. The current experiments investigated the effects of varying the timing of the estrogen injection on ovum transport rates. Fifty-eight rabbits received injections of either Depot-estradiol or crystalline estradiol at various times before or after the injection of HCG. \"Tube-locking\" resulted when estrogen was administered before or at the time of HCG administration, whereas acceleration occurred when estrogen was given 12 to 24 hours after HCG. The results demonstrate that pharmacologic doses of estrogen exert two effects on ovum transport in the rabbit: (1) delay at the ampullary-isthmic junction and (2) acceleration once that junction has been traversed. The possible application of these results to contraception is discussed.", "contents": "Temporal relationships critical to estrogen-induced delay of ovum transport. Previous studies have shown that a pharmacologic dose of estradiol given at the time of injection of human chorionic gonadotropin (HCG) significantly delays the transport of ova through the rabbit oviduct and that the site of delay is located at the ampullary-isthmic junction. The current experiments investigated the effects of varying the timing of the estrogen injection on ovum transport rates. Fifty-eight rabbits received injections of either Depot-estradiol or crystalline estradiol at various times before or after the injection of HCG. \"Tube-locking\" resulted when estrogen was administered before or at the time of HCG administration, whereas acceleration occurred when estrogen was given 12 to 24 hours after HCG. The results demonstrate that pharmacologic doses of estrogen exert two effects on ovum transport in the rabbit: (1) delay at the ampullary-isthmic junction and (2) acceleration once that junction has been traversed. The possible application of these results to contraception is discussed.", "PMID": 976504}
{"id": "pubmed23n0032_10746", "title": "Effect of direct injection of neutrophils from uterine horns of rats containing intrauterine devices into recipient pregnant uteri.", "content": "The purpose of this study was to determine whether or not neutrophils from uterine horns containing an intruterine device (IUD) inhibit implantation of rat blastocysts. On day 4 (6:00 P.M. to 8:00 P.M.) of gestation the uterine horns of the recipient rats were exposed and injected with neutrophils, supernatant luminal fluid, or Hanks' solution. The total volume injected was 30 mul. All of the rats were killed on day 12 of pregnancy, and the number of fetuses and resorption sites was determined. The data show that, in rats injected with neutrophils on day 4 of gestation, implantation was significantly increased as compared with controls injected with Hanks' (vehicle) solution. Whether the supernatant fluid had an effect in suppression of implantation could not be determined, since the effect was not statistically different from that observed in either the vehicle- or neutrophil-injected groups. However, the responses observed with the supernatant-injected groups. However, the responses observed with the supernatant-injected group were intermediate between those of the control horns and those of the neutrophil-injected horns. The finding supports the concept that in IUD-bearing animals in which there is a rapid influx of neutrophils the cells play a major role in suppressing implantation.", "contents": "Effect of direct injection of neutrophils from uterine horns of rats containing intrauterine devices into recipient pregnant uteri. The purpose of this study was to determine whether or not neutrophils from uterine horns containing an intruterine device (IUD) inhibit implantation of rat blastocysts. On day 4 (6:00 P.M. to 8:00 P.M.) of gestation the uterine horns of the recipient rats were exposed and injected with neutrophils, supernatant luminal fluid, or Hanks' solution. The total volume injected was 30 mul. All of the rats were killed on day 12 of pregnancy, and the number of fetuses and resorption sites was determined. The data show that, in rats injected with neutrophils on day 4 of gestation, implantation was significantly increased as compared with controls injected with Hanks' (vehicle) solution. Whether the supernatant fluid had an effect in suppression of implantation could not be determined, since the effect was not statistically different from that observed in either the vehicle- or neutrophil-injected groups. However, the responses observed with the supernatant-injected groups. However, the responses observed with the supernatant-injected group were intermediate between those of the control horns and those of the neutrophil-injected horns. The finding supports the concept that in IUD-bearing animals in which there is a rapid influx of neutrophils the cells play a major role in suppressing implantation.", "PMID": 976505}
{"id": "pubmed23n0032_10747", "title": "The hormonal control of the guinea pig corpus luteum during early pregnancy.", "content": "Forty guinea pigs were divided into two groups, pregnant and nonpregnant, and were operated upon on days 10, 12, 14, and 16 of the ovarian cycle or of pregnancy. Blood was obtained from the utero-ovarian vein, and prostaglandin F2alpha (PGF2alpha), prostaglandin E (PGE), progesterone, and estrogen content was assayed by radioimmunoassay. Increased levels of PGF2alpha preceded a drop in progesterone levels in nonpregnant animals, reflecting luteolysis. In pregnant animals, the level of PGF2alpha remained low, and progesterone levels increased. PGE levels were similar in the two groups. These results suggest that the low PGF2alpha levels seen during early pregnancy are related to corpus luteum rescue.", "contents": "The hormonal control of the guinea pig corpus luteum during early pregnancy. Forty guinea pigs were divided into two groups, pregnant and nonpregnant, and were operated upon on days 10, 12, 14, and 16 of the ovarian cycle or of pregnancy. Blood was obtained from the utero-ovarian vein, and prostaglandin F2alpha (PGF2alpha), prostaglandin E (PGE), progesterone, and estrogen content was assayed by radioimmunoassay. Increased levels of PGF2alpha preceded a drop in progesterone levels in nonpregnant animals, reflecting luteolysis. In pregnant animals, the level of PGF2alpha remained low, and progesterone levels increased. PGE levels were similar in the two groups. These results suggest that the low PGF2alpha levels seen during early pregnancy are related to corpus luteum rescue.", "PMID": 976506}
{"id": "pubmed23n0032_10748", "title": "Age-related changes in male rat reproductive organ weights and plasma testosterone concentrations after administration of a monoamine oxidase inhibitor.", "content": "Experiments were conducted to determine the effects of pargyline, a monoamine oxidase inhibitor, onmale rat reproductive organ weights, testicular histology, and plasma testosterone levels. Pargyline was administered to various groups of rats by implanting it, either dissolved or in a powder form, in Silastic tubing capsules. Additional rats were injected with pargyline solutions. The effects of pargyline.appeared to be age-related with younger animals, weighing under 400 gm (less than 100 days of age), showing a decrease in testicular and accessory organ weights and plasma testosterone. Older animals did not show a decrease in organ weights. Testicular histology in the pargyline-treated groups showed sloughage of cellular material into the tubular lumina, decreases in the number of cell layers, and a reduction of the number of mature spermatocytes. Other areas in the same testis were normal, however, and resembled control histology. The results suggest that pargyline can have detrimental effects on testicular function and that the effects may be age-related.", "contents": "Age-related changes in male rat reproductive organ weights and plasma testosterone concentrations after administration of a monoamine oxidase inhibitor. Experiments were conducted to determine the effects of pargyline, a monoamine oxidase inhibitor, onmale rat reproductive organ weights, testicular histology, and plasma testosterone levels. Pargyline was administered to various groups of rats by implanting it, either dissolved or in a powder form, in Silastic tubing capsules. Additional rats were injected with pargyline solutions. The effects of pargyline.appeared to be age-related with younger animals, weighing under 400 gm (less than 100 days of age), showing a decrease in testicular and accessory organ weights and plasma testosterone. Older animals did not show a decrease in organ weights. Testicular histology in the pargyline-treated groups showed sloughage of cellular material into the tubular lumina, decreases in the number of cell layers, and a reduction of the number of mature spermatocytes. Other areas in the same testis were normal, however, and resembled control histology. The results suggest that pargyline can have detrimental effects on testicular function and that the effects may be age-related.", "PMID": 976507}
{"id": "pubmed23n0032_10749", "title": "The effects of hormones and prostaglandins on the calcium pools in cultured myocardial cells.", "content": "Determinations of the calcium pools in myocardial cells in vitro have shown the existence of at least three pools of exchangeable calcium. Epinephrine and glucagon were found to produce significant increases in the size of the two slower exchanging pools. Prostaglandins E1 and F1alpha also increased significantly calcium pool size whereas E2 and F2alpha did not; results which correlate well with the effects of the two former prostaglandins on intracellular cAMP levels. The results imply that these agents cause small, but significant, changes in the transmembrane exchange of calcium and large increases in the intracellular calcium pool. Effects which may involve the direct or indirect action of cAMP.", "contents": "The effects of hormones and prostaglandins on the calcium pools in cultured myocardial cells. Determinations of the calcium pools in myocardial cells in vitro have shown the existence of at least three pools of exchangeable calcium. Epinephrine and glucagon were found to produce significant increases in the size of the two slower exchanging pools. Prostaglandins E1 and F1alpha also increased significantly calcium pool size whereas E2 and F2alpha did not; results which correlate well with the effects of the two former prostaglandins on intracellular cAMP levels. The results imply that these agents cause small, but significant, changes in the transmembrane exchange of calcium and large increases in the intracellular calcium pool. Effects which may involve the direct or indirect action of cAMP.", "PMID": 976590}
{"id": "pubmed23n0032_10750", "title": "Steroids and growth of a human cell line stemming from a carcinoma of the uterine cervix.", "content": "The effect of different steroids on the growth of a human cell line, was examined in NHIK 3025 cells, derived from a carcinoma of the uterine cervix. When this cell line was grown in Eagle's minimal essential medium (MEM) for 4 days, addition to the medium of estradiol-17 beta in concentrations ranging from 10(-9) to 10(-7) M did not promote significant cell growth. Stimulated growth was observed with testosterone in these concentrations, with maximal effect (29% above control) at 10(-7) M. 5alpha-Dihydrotestosterone over the range 10(-9)--10(-6) M gave no such effect. 4-Androstene-3,17-dione, 5alpha-androstan-3alpha,17beta-diol and 5alpha-androstan-3beta,17beta-diol had no significant effect on cell proliferation at a concentration of 10(-7) M, while 4-androstene-3beta,17beta-diol augmented cell number to values 50-60% higher than those of control cultures. The observed increase in cell number was due to a shortening of the cell cycle and to a higher plating efficiency. Thus, cell line NHIK 3025 has not the responsiveness to estradiol-17beta of normal uterine cells. The data suggest that testosterone and/or 4-androstene-3beta,17beta-diol may be growth factors for this cell line.", "contents": "Steroids and growth of a human cell line stemming from a carcinoma of the uterine cervix. The effect of different steroids on the growth of a human cell line, was examined in NHIK 3025 cells, derived from a carcinoma of the uterine cervix. When this cell line was grown in Eagle's minimal essential medium (MEM) for 4 days, addition to the medium of estradiol-17 beta in concentrations ranging from 10(-9) to 10(-7) M did not promote significant cell growth. Stimulated growth was observed with testosterone in these concentrations, with maximal effect (29% above control) at 10(-7) M. 5alpha-Dihydrotestosterone over the range 10(-9)--10(-6) M gave no such effect. 4-Androstene-3,17-dione, 5alpha-androstan-3alpha,17beta-diol and 5alpha-androstan-3beta,17beta-diol had no significant effect on cell proliferation at a concentration of 10(-7) M, while 4-androstene-3beta,17beta-diol augmented cell number to values 50-60% higher than those of control cultures. The observed increase in cell number was due to a shortening of the cell cycle and to a higher plating efficiency. Thus, cell line NHIK 3025 has not the responsiveness to estradiol-17beta of normal uterine cells. The data suggest that testosterone and/or 4-androstene-3beta,17beta-diol may be growth factors for this cell line.", "PMID": 976591}
{"id": "pubmed23n0032_10751", "title": "Ovarian-independent fluctuations of estradiol receptor levels in mammalian tissues.", "content": "Three types of periodic fluctuation in tissue concentrations of estradiol receptor protein have been observed. A seasonal variation is described in the uteri of 12-16-week-old calves and of ovariectomized pigs, and in mammary tumor tissue obtained from postmenopausal women. A circadian rhythm has been demonstrated in uteri of ovariectomized rats. An irregular periodic fluctuation has been found in uteri of ovariectomized and of ovariectomized/hypophysectomized rats, with the period varying from 9 to 15 days. These observations establish that a substantial turnover of receptor occurs in the absence of hormone and that 'normal' baseline values of receptor concentration do not exist.", "contents": "Ovarian-independent fluctuations of estradiol receptor levels in mammalian tissues. Three types of periodic fluctuation in tissue concentrations of estradiol receptor protein have been observed. A seasonal variation is described in the uteri of 12-16-week-old calves and of ovariectomized pigs, and in mammary tumor tissue obtained from postmenopausal women. A circadian rhythm has been demonstrated in uteri of ovariectomized rats. An irregular periodic fluctuation has been found in uteri of ovariectomized and of ovariectomized/hypophysectomized rats, with the period varying from 9 to 15 days. These observations establish that a substantial turnover of receptor occurs in the absence of hormone and that 'normal' baseline values of receptor concentration do not exist.", "PMID": 976592}
{"id": "pubmed23n0032_10752", "title": "Gastric inhibitory polypeptide (GIP) in maturity-onset diabetes mellitus.", "content": "Serum GIP, insulin, and glucose concentrations were determined during a standard oral glucose tolerance test in 80 individuals, 45 of whom were normal and 35 of whom had adult-onset diabetes mellitus according to USPHS criteria. As a group, the diabetics had fasting hyperglycemia (219 +/- 17 mg./dl.) and, in response to glucose, displayed a peak serum glucose of 373 +/- 23 mg./dl. and sustained hyperglycemia (315 +/- 24 mg./dl.) at 180 minutes. There were no statistically significant differences in absolute serum insulin levels between the two groups. However, insulin secretion was delayed, IRI increments were smaller, and the IRI concentrations were inappropriately low for the simultaneous serum glucose concentrations in the diabetics at every time interval tested. Mean fasting serum GIP was 335 +/- 30 pg./ml. in the diabetics as against 262 +/- 15 pg./ml. in normal individuals (p less than 0.025). After the ingestion of glucose, diabetics had significantly higher (p less than 0.001) mean serum GIP levels between five and 120 minutes. By 180 minutes, serum GIP levels remained above fasting in both groups, but the diabetics had higher than normal serum concentrations (p less than 0.05). Peak serum GIP concentrations, which occurred at 30 minutes in both groups, were 1,376 +/- 106 and 806 +/- 75 pg./ml. in the diabetics and normals, respectively (p less than 0.001). Total integrated serum GIP was also greater in diabetics than normals (140,852 +/- 14,208 vs. 64,602 +/- 8,719 pg.-min./ml.-1, p less than 0.001). The higher serum GIP concentrations observed following glucose ingestion in diabetics could not be attributed to obesity or age. We conclude that both fasting and glucose-stimulated GIP concentrations are higher than normal in obese adult-onset diabetics. The significance of this observation is uncertain. However, since our current understanding suggests the GIP may be an important enteric signal for the release of insulin in man, and because GIP has been shown to stimulate the release of immunoreactive glucagon, GIP may play a role in the pathogenesis of diabetes mellitus.", "contents": "Gastric inhibitory polypeptide (GIP) in maturity-onset diabetes mellitus. Serum GIP, insulin, and glucose concentrations were determined during a standard oral glucose tolerance test in 80 individuals, 45 of whom were normal and 35 of whom had adult-onset diabetes mellitus according to USPHS criteria. As a group, the diabetics had fasting hyperglycemia (219 +/- 17 mg./dl.) and, in response to glucose, displayed a peak serum glucose of 373 +/- 23 mg./dl. and sustained hyperglycemia (315 +/- 24 mg./dl.) at 180 minutes. There were no statistically significant differences in absolute serum insulin levels between the two groups. However, insulin secretion was delayed, IRI increments were smaller, and the IRI concentrations were inappropriately low for the simultaneous serum glucose concentrations in the diabetics at every time interval tested. Mean fasting serum GIP was 335 +/- 30 pg./ml. in the diabetics as against 262 +/- 15 pg./ml. in normal individuals (p less than 0.025). After the ingestion of glucose, diabetics had significantly higher (p less than 0.001) mean serum GIP levels between five and 120 minutes. By 180 minutes, serum GIP levels remained above fasting in both groups, but the diabetics had higher than normal serum concentrations (p less than 0.05). Peak serum GIP concentrations, which occurred at 30 minutes in both groups, were 1,376 +/- 106 and 806 +/- 75 pg./ml. in the diabetics and normals, respectively (p less than 0.001). Total integrated serum GIP was also greater in diabetics than normals (140,852 +/- 14,208 vs. 64,602 +/- 8,719 pg.-min./ml.-1, p less than 0.001). The higher serum GIP concentrations observed following glucose ingestion in diabetics could not be attributed to obesity or age. We conclude that both fasting and glucose-stimulated GIP concentrations are higher than normal in obese adult-onset diabetics. The significance of this observation is uncertain. However, since our current understanding suggests the GIP may be an important enteric signal for the release of insulin in man, and because GIP has been shown to stimulate the release of immunoreactive glucagon, GIP may play a role in the pathogenesis of diabetes mellitus.", "PMID": 976601}
{"id": "pubmed23n0032_10753", "title": "Epidemiologic findings on the relationship of time of day and time since last meal to glucose tolerance.", "content": "Data from 10,559 men and women, age 30-64, participating in the morning and afternoon in a Chicago Health Department multiphasic screening project, were used to determine the effects of time of day and time since last meal on the values for plasma glucose one and two hours following oral challenge with 100 gm. of glucose. Mean plasma glucose values and rates of suspect glucose intolerance (based on several cutpoints) were sizeably higher in the afternoon than in the morning. In addition, plasma glucose values increased with time elapsed since the last meal, up to 10 hours postprandially. Thereafter, both one- and two-hour plasma glucose values tended to exhibit a decline. Analysis of covariance confirmed that fluctuations in glucose tolerance were related to time of day and time since last meal, but the effects of each parameter were exerted independently.", "contents": "Epidemiologic findings on the relationship of time of day and time since last meal to glucose tolerance. Data from 10,559 men and women, age 30-64, participating in the morning and afternoon in a Chicago Health Department multiphasic screening project, were used to determine the effects of time of day and time since last meal on the values for plasma glucose one and two hours following oral challenge with 100 gm. of glucose. Mean plasma glucose values and rates of suspect glucose intolerance (based on several cutpoints) were sizeably higher in the afternoon than in the morning. In addition, plasma glucose values increased with time elapsed since the last meal, up to 10 hours postprandially. Thereafter, both one- and two-hour plasma glucose values tended to exhibit a decline. Analysis of covariance confirmed that fluctuations in glucose tolerance were related to time of day and time since last meal, but the effects of each parameter were exerted independently.", "PMID": 976602}
{"id": "pubmed23n0032_10754", "title": "Potentiation of glucose-induced insulin release by glucose in the isolated pancreas of fed and fasted rats.", "content": "Glucose, apart from its acute insulin-releasing effect, exerts a time-dependent potentiating action on subsequent stimulations of the pancreas. The influence of a 24-hour starvation on these two actions of glucose was studied with the completely isolated, perfused rat pancreas preparation. Starvation had no effect on the time kinetics of the insulin response, but the magnitudes of the early and late insulin-secretion phases were reduced to similar extents. It was demonstrated by using a wide glucose concentration range that the maximal insulin response is not significantly modified by starvation. In contrast, both the threshold of stimulation by and the Km for glucose were higher in the pancrease from fasted rats. Thus, starvation reduces the sensitivity of the islet for the insulin-releasing action of glucose. When the stimulatory concentrations of glucose were preceded for 40 minutes by the perfusion of 8.3 mM instead of the basal, 4.4 mM glucose, insulin secretion from the pancreas of fed animals was not modified. In contrast, raising the glucose concentration of the equilibrium period to 8.3 mM potentiated markedly the insulin response to subsequent stimulations in the pancreas from fasted rats. This potentiation expressed itself as increase in the maximal response: the Km for glucose was not reduced. Thus a 40-minute pretreatment with 8.3 mM glucose does not correct the diminished sensitivity induced by a 24-hour starvation. It is concluded that in starvation (1) the sensitivity for glucose of the mechanisms that initiate insulin release is diminished; (2) the sensitivity of the pancreas for the potentiation-inducing action of glucose is augumented. (3) In both respects, the insulin response of fasted rats is similar to that of mildly diabetic subjects. These and other findings suggest that the effect of glucose in initiation of insulin release and on generation of a state of potentiation in the islet are mediated by different mechanisms.", "contents": "Potentiation of glucose-induced insulin release by glucose in the isolated pancreas of fed and fasted rats. Glucose, apart from its acute insulin-releasing effect, exerts a time-dependent potentiating action on subsequent stimulations of the pancreas. The influence of a 24-hour starvation on these two actions of glucose was studied with the completely isolated, perfused rat pancreas preparation. Starvation had no effect on the time kinetics of the insulin response, but the magnitudes of the early and late insulin-secretion phases were reduced to similar extents. It was demonstrated by using a wide glucose concentration range that the maximal insulin response is not significantly modified by starvation. In contrast, both the threshold of stimulation by and the Km for glucose were higher in the pancrease from fasted rats. Thus, starvation reduces the sensitivity of the islet for the insulin-releasing action of glucose. When the stimulatory concentrations of glucose were preceded for 40 minutes by the perfusion of 8.3 mM instead of the basal, 4.4 mM glucose, insulin secretion from the pancreas of fed animals was not modified. In contrast, raising the glucose concentration of the equilibrium period to 8.3 mM potentiated markedly the insulin response to subsequent stimulations in the pancreas from fasted rats. This potentiation expressed itself as increase in the maximal response: the Km for glucose was not reduced. Thus a 40-minute pretreatment with 8.3 mM glucose does not correct the diminished sensitivity induced by a 24-hour starvation. It is concluded that in starvation (1) the sensitivity for glucose of the mechanisms that initiate insulin release is diminished; (2) the sensitivity of the pancreas for the potentiation-inducing action of glucose is augumented. (3) In both respects, the insulin response of fasted rats is similar to that of mildly diabetic subjects. These and other findings suggest that the effect of glucose in initiation of insulin release and on generation of a state of potentiation in the islet are mediated by different mechanisms.", "PMID": 976603}
{"id": "pubmed23n0032_10755", "title": "Effects of acute insulin withdrawal and administration on plasma glucagon responses to intravenous arginine in insulin-dependent diabetic subjects.", "content": "To assess further the role of insulin in the abnormal alpha-cell dysfunction found in human diabetes mellitus, the effects of acute insulin withdrawal and administration on plasma glucagon responses to intravenous arginine were studied in eight insulin-dependent diabetic subjects. Arginine infusions (30 gm. over 30 minutes) were performed during and at one and four hours after discontinuation of a 14-hour insulin infusion (1.5 U. per hour), which had rendered the subjects euglycemic, and on another occasion before and one and four hours into a five-hour infusion of insulin (1.5 U. per hour). During the last hour of the 14-hour infusion, glucagon responses to arginine (area under the curve, nanograms per milliliter per minute) were similar to those found in normal subjects (10.3 +/- 0.8 vs. 9.0 +/- 0.8, respectively). After discontinuation of the insulin infusions, glucagon responses increased progressively (p less than 0.01) to values (16.8 +/- 1.2) that exceeded those of normal subjects by four hours (p less than 0.01). These were similar to results found in the same subjects studied when their diabetes was in less than optimal control (14.9 +/- 1.3). Infusion of insulin under these conditions progressively decreased glucagon responses to arginine to values (9.6 +/- 0.8; p less than 0.01) that, at four hours, were similar to those of normal subjects and to values found at the end of the 14-hour infusion of insulin in the same diabetic individuals. These results demonstrate a rapid effect of insulin on glucagon responses to arginine and suggest that the abnormal responses seen in diabetes mellitus are the immediate result of insulin deficiency. Since abnormal glucagon responses to glucose in diabetes are not as readily corrected by insulin, the mechanisms underlying the abnormal responses to these two stimuli may differ.", "contents": "Effects of acute insulin withdrawal and administration on plasma glucagon responses to intravenous arginine in insulin-dependent diabetic subjects. To assess further the role of insulin in the abnormal alpha-cell dysfunction found in human diabetes mellitus, the effects of acute insulin withdrawal and administration on plasma glucagon responses to intravenous arginine were studied in eight insulin-dependent diabetic subjects. Arginine infusions (30 gm. over 30 minutes) were performed during and at one and four hours after discontinuation of a 14-hour insulin infusion (1.5 U. per hour), which had rendered the subjects euglycemic, and on another occasion before and one and four hours into a five-hour infusion of insulin (1.5 U. per hour). During the last hour of the 14-hour infusion, glucagon responses to arginine (area under the curve, nanograms per milliliter per minute) were similar to those found in normal subjects (10.3 +/- 0.8 vs. 9.0 +/- 0.8, respectively). After discontinuation of the insulin infusions, glucagon responses increased progressively (p less than 0.01) to values (16.8 +/- 1.2) that exceeded those of normal subjects by four hours (p less than 0.01). These were similar to results found in the same subjects studied when their diabetes was in less than optimal control (14.9 +/- 1.3). Infusion of insulin under these conditions progressively decreased glucagon responses to arginine to values (9.6 +/- 0.8; p less than 0.01) that, at four hours, were similar to those of normal subjects and to values found at the end of the 14-hour infusion of insulin in the same diabetic individuals. These results demonstrate a rapid effect of insulin on glucagon responses to arginine and suggest that the abnormal responses seen in diabetes mellitus are the immediate result of insulin deficiency. Since abnormal glucagon responses to glucose in diabetes are not as readily corrected by insulin, the mechanisms underlying the abnormal responses to these two stimuli may differ.", "PMID": 976604}
{"id": "pubmed23n0032_10756", "title": "Imparied plasma insulin response to arginine in hyperthyroidism. Important role of the rise of blood glucose in the second phase of insulin release induced by argiinine.", "content": "Intravenous infusion of 30 gm. of L-arginine over a period of 45 minutes elicited a biphasic insulin response and a moderate blood glucose rise in normal subjects. In patients with hyperthyroidism, both insulin peaks, especially the second one, were low, with virtual absence of the blood glucose response. A single intravenous injection of 4 gm. of arginine provoked similar uniphasic plasma insulin responses in both normal subjects and hyperthyroid patients. Pretreatment with either glucose or xylitol almost completely restored the biphasic insulin response to arginine in patients with hyperthyroidism, whereas pretreatment with aminophylline only partially improved the insulin response. Combined administration of a small amount of glucose (2.25 mg./kg./min.) with arginine also restored the normal second-phase insulin release, with blood glucose rises similar to those in normal subjects given arginine alone. It is concluded that the plasma insulin response to arginine is impaired, especially in its second phase, in patients with hyperthyroidism due to the absence of a blood glucose rise. The second phase of arginine-induced insulin release seems more dependent on glucose than the first phase.", "contents": "Imparied plasma insulin response to arginine in hyperthyroidism. Important role of the rise of blood glucose in the second phase of insulin release induced by argiinine. Intravenous infusion of 30 gm. of L-arginine over a period of 45 minutes elicited a biphasic insulin response and a moderate blood glucose rise in normal subjects. In patients with hyperthyroidism, both insulin peaks, especially the second one, were low, with virtual absence of the blood glucose response. A single intravenous injection of 4 gm. of arginine provoked similar uniphasic plasma insulin responses in both normal subjects and hyperthyroid patients. Pretreatment with either glucose or xylitol almost completely restored the biphasic insulin response to arginine in patients with hyperthyroidism, whereas pretreatment with aminophylline only partially improved the insulin response. Combined administration of a small amount of glucose (2.25 mg./kg./min.) with arginine also restored the normal second-phase insulin release, with blood glucose rises similar to those in normal subjects given arginine alone. It is concluded that the plasma insulin response to arginine is impaired, especially in its second phase, in patients with hyperthyroidism due to the absence of a blood glucose rise. The second phase of arginine-induced insulin release seems more dependent on glucose than the first phase.", "PMID": 976605}
{"id": "pubmed23n0032_10757", "title": "Plasma renin activity and hypertension in diabetes mellitus.", "content": "Plasma renin activity (PRA) was determined in 48 patients with diabetes mellitus in sodium balance on a 10-20 mEq. Na diet. Nine were normotensive (group I), 11 11 were hypertensive without diabetic nephropathy (group III). Results were compared with those in 16 normal subjects and 49 nondiabetic patients with essential hypertension in similar Na balance. Mean supine PRA did not differ significantly among groups I and II, normal subjects, and patients with essential hypertension. Group III diabetics had a supine PRA of 2.4 +/- 0.4 ng./ml./hr. (x +/- S.E.M.), significantly lower than the other diabetic groups (P less than 0.005) and normal subjects (P less than 0.05). Upright PRA was 12.8 +/- 2.2 in group I diabetics, similar to that in normal subjects (13.3 +/- 2.3), and 8.1 +/- 1.4 in group II diabetics, similar to that in essential hypertensives (6.8 +/- 0.8). In group III diabetics, upright PRA was 4.0 +/- 0.5, significantly lower than that in any other group. These results suggest that (1) PRA is normal in normotensive diabetics, (2) upright PRA in diabetics with hypertension but no nephropathy is similar to that in essential hypertension, and (3) patients with diabetes, hypertension, and nephropathy have \"low renin hypertension,\" explaining the virtual absence of malignant hypertension in this group. Although the major mechanism for this low PRA may be volume expansion, indicating the need for potent diuretics, other mechanisms include hyalinization of the afferent arteriole, decreased cathecholamine stimulation of renin release, and inadequate conversion of prorenin to renin.", "contents": "Plasma renin activity and hypertension in diabetes mellitus. Plasma renin activity (PRA) was determined in 48 patients with diabetes mellitus in sodium balance on a 10-20 mEq. Na diet. Nine were normotensive (group I), 11 11 were hypertensive without diabetic nephropathy (group III). Results were compared with those in 16 normal subjects and 49 nondiabetic patients with essential hypertension in similar Na balance. Mean supine PRA did not differ significantly among groups I and II, normal subjects, and patients with essential hypertension. Group III diabetics had a supine PRA of 2.4 +/- 0.4 ng./ml./hr. (x +/- S.E.M.), significantly lower than the other diabetic groups (P less than 0.005) and normal subjects (P less than 0.05). Upright PRA was 12.8 +/- 2.2 in group I diabetics, similar to that in normal subjects (13.3 +/- 2.3), and 8.1 +/- 1.4 in group II diabetics, similar to that in essential hypertensives (6.8 +/- 0.8). In group III diabetics, upright PRA was 4.0 +/- 0.5, significantly lower than that in any other group. These results suggest that (1) PRA is normal in normotensive diabetics, (2) upright PRA in diabetics with hypertension but no nephropathy is similar to that in essential hypertension, and (3) patients with diabetes, hypertension, and nephropathy have \"low renin hypertension,\" explaining the virtual absence of malignant hypertension in this group. Although the major mechanism for this low PRA may be volume expansion, indicating the need for potent diuretics, other mechanisms include hyalinization of the afferent arteriole, decreased cathecholamine stimulation of renin release, and inadequate conversion of prorenin to renin.", "PMID": 976606}
{"id": "pubmed23n0032_10758", "title": "Modulation of fatty acid metabolism by glucagon in man. IV. Effects of a physiologic hormone infusion in normal man.", "content": "This study was done to explore the role of physiologic elevations of glucagon concentration in plasma ketone body concentration in normal man. During the period of hormone elevation, plasma free fatty acids were pharmacologically elevated to ensure adequate free fatty acid substrate delivery to the liver to support hepatic ketogenesis. Eighty-minute infusions of glucagon resulted in a plasma hormone concentration of approximately 300 pg./ml. During the infusion, ketone bodies declined from their basal concentration and remained below basal for the duration of the infusion. An acute heparin-induced pharmacologic elevation of plasma free fatty acid concentration resulted in a transient rise in plasma ketone body concentration, but at no time did it attain the concentration observed during the control saline infusion. Plasma glucose concentration was not altered by glucagon infusion, but plasma insulin concentration rose by approximately 2.5 muU./ml. These results suggest that glucagon is not ketogenic in normal man as has been previously reported in insulin-deficient diabetics. The glucagon-induced rise in plasma insulin concentration may participate in the observed reduction in plasma ketone body concentration.", "contents": "Modulation of fatty acid metabolism by glucagon in man. IV. Effects of a physiologic hormone infusion in normal man. This study was done to explore the role of physiologic elevations of glucagon concentration in plasma ketone body concentration in normal man. During the period of hormone elevation, plasma free fatty acids were pharmacologically elevated to ensure adequate free fatty acid substrate delivery to the liver to support hepatic ketogenesis. Eighty-minute infusions of glucagon resulted in a plasma hormone concentration of approximately 300 pg./ml. During the infusion, ketone bodies declined from their basal concentration and remained below basal for the duration of the infusion. An acute heparin-induced pharmacologic elevation of plasma free fatty acid concentration resulted in a transient rise in plasma ketone body concentration, but at no time did it attain the concentration observed during the control saline infusion. Plasma glucose concentration was not altered by glucagon infusion, but plasma insulin concentration rose by approximately 2.5 muU./ml. These results suggest that glucagon is not ketogenic in normal man as has been previously reported in insulin-deficient diabetics. The glucagon-induced rise in plasma insulin concentration may participate in the observed reduction in plasma ketone body concentration.", "PMID": 976607}
{"id": "pubmed23n0032_10759", "title": "Blood glucose monitoring in symptomatic hypoglycemia.", "content": "The relationship between blood glucose levels and the onset of hypoglycemic symptoms was studied by continuous monitoring of blood glucose levels after an oral glucose load in nine adults with normal glucose tolerance, five with chemical diabetes without symptomatic hypoglycemia, and nine with chemical diabetes with symptomatic hypoglycemia. Symptoms were associated not only with a low level of blood glucose but with a rapid fall as well. These two parameters were used to calculate a \"hypoglycemic index\" (defined as the fall in blood glucose during a 90-minute period prior to reaching the minimum level, divided by the value of this minimum level). The hypoglycemic index was 2.3 +/- 0.6 (mean +/- S.D.) in the group of diabetic patients with symptomatic reactive hypoglycemia and 0.7 +/- 0.3 for the other groups. This index may aid in the diagnosis of patients with symptoms of hypoglycemia and equivocally low values of blood glucose.", "contents": "Blood glucose monitoring in symptomatic hypoglycemia. The relationship between blood glucose levels and the onset of hypoglycemic symptoms was studied by continuous monitoring of blood glucose levels after an oral glucose load in nine adults with normal glucose tolerance, five with chemical diabetes without symptomatic hypoglycemia, and nine with chemical diabetes with symptomatic hypoglycemia. Symptoms were associated not only with a low level of blood glucose but with a rapid fall as well. These two parameters were used to calculate a \"hypoglycemic index\" (defined as the fall in blood glucose during a 90-minute period prior to reaching the minimum level, divided by the value of this minimum level). The hypoglycemic index was 2.3 +/- 0.6 (mean +/- S.D.) in the group of diabetic patients with symptomatic reactive hypoglycemia and 0.7 +/- 0.3 for the other groups. This index may aid in the diagnosis of patients with symptoms of hypoglycemia and equivocally low values of blood glucose.", "PMID": 976608}
{"id": "pubmed23n0032_10760", "title": "Renal wastage of insulin in children with diabetes mellitus.", "content": "In normal human beings the percentage of serum insulin excreted in the urine is constant over a wide range of values. The quantity of immunoreactive insulin found in the urine is believed to reflect the level of free insulin in the serum. Immunoreactive insulin was measured in the urine of nondiabetic children and diabetic children receiving exogenous insulin. Children with diabetes mellitus excreted greater amounts of immunoreactive insulin (18.5+/-8 muU./mg. creatinine) than did nondiabetic children (11.9+/-5 muU./mg. creatinine). This difference was statistically significant (p less than 0.0005). Children with \"poor glycemic control\" excreted a greater portion of their administered insulin dose than did those with \"good control.\" The renal wastage of insulin correlated (r=0.94) with the duration of insulin treatment but not with the quantity administered. Antibody binding of serum insulin may explain in part these observations, but an acquired defect in the renal tubular reabsorption of insulin may also exist. Modifications in the management of diabetes that reduce the renal wastage of insulin may improve the metabolic stability of children with \"poor diabetic control.\"", "contents": "Renal wastage of insulin in children with diabetes mellitus. In normal human beings the percentage of serum insulin excreted in the urine is constant over a wide range of values. The quantity of immunoreactive insulin found in the urine is believed to reflect the level of free insulin in the serum. Immunoreactive insulin was measured in the urine of nondiabetic children and diabetic children receiving exogenous insulin. Children with diabetes mellitus excreted greater amounts of immunoreactive insulin (18.5+/-8 muU./mg. creatinine) than did nondiabetic children (11.9+/-5 muU./mg. creatinine). This difference was statistically significant (p less than 0.0005). Children with \"poor glycemic control\" excreted a greater portion of their administered insulin dose than did those with \"good control.\" The renal wastage of insulin correlated (r=0.94) with the duration of insulin treatment but not with the quantity administered. Antibody binding of serum insulin may explain in part these observations, but an acquired defect in the renal tubular reabsorption of insulin may also exist. Modifications in the management of diabetes that reduce the renal wastage of insulin may improve the metabolic stability of children with \"poor diabetic control.\"", "PMID": 976609}
{"id": "pubmed23n0032_10761", "title": "Environmental effects on motor development: the case of \"African infant precocity\".", "content": "In order to clarify the extent and cause of African infants' precocity in motor development, as reported by Geber and others, 64 babies and their families were intensively studied in a rural Kenyan community. It was found that the motor skills of sitting and walking, which the Kenyan babies acquired early (by American standards), are (a) specifically taught by the caretakers and (b) can be practised in the course of their usual daily routines. They are not advanced in skills which are not taught or practised. Middle-class urban Kenyan children from the same ethnic background were found generally to be intermediate in both environmental encouragement and rate of advancement. Preliminary results from other groups in Kenya suggest that encouragement of motor development is widespread and that for behaviors which are differentially encouraged among groups, the average age of attainment is predictable from environmental measures.", "contents": "Environmental effects on motor development: the case of \"African infant precocity\". In order to clarify the extent and cause of African infants' precocity in motor development, as reported by Geber and others, 64 babies and their families were intensively studied in a rural Kenyan community. It was found that the motor skills of sitting and walking, which the Kenyan babies acquired early (by American standards), are (a) specifically taught by the caretakers and (b) can be practised in the course of their usual daily routines. They are not advanced in skills which are not taught or practised. Middle-class urban Kenyan children from the same ethnic background were found generally to be intermediate in both environmental encouragement and rate of advancement. Preliminary results from other groups in Kenya suggest that encouragement of motor development is widespread and that for behaviors which are differentially encouraged among groups, the average age of attainment is predictable from environmental measures.", "PMID": 976610}
{"id": "pubmed23n0032_10762", "title": "Testing of visual acuity in young children: an evaluation of some commonly used methods.", "content": "In a comparative study of screening tests for visual acuity in young children, two groups of children were presented with three different tests. In the younger group (three to five years) three single opto-type tests were compared: Sheridan's five and seven letter matching test, the Fooks test and the E card test. The Sheridan test gave the best results and the E card test was found to be unsuitable for this age-group. The Fooks is an attractive test but was less sensitive in the detection of defects than the Sheridan test. In the older group (five to seven years) a single opto-type test, the Sheridan-Gardiner test, was compared with the E chart and the Snellen chart. The Sheridan-Gardiner test was found to have limitations in the detection of defects, including amblyopia, therefore results obtained by this method should not be considered to be directly comparable with those of the Snellen chart. The E chart gave good results in the detection of defects and there was less lateral confusion than had been expected. It has disadvantages, but there is still a place for its use in the screening of normal school-age children. The Snellen chart was the most effective test in the detection of defects, but not all the children were able to co-operate in its use. It is concluded that where it is possible to use the Snellen chart it should always be the method of choice.", "contents": "Testing of visual acuity in young children: an evaluation of some commonly used methods. In a comparative study of screening tests for visual acuity in young children, two groups of children were presented with three different tests. In the younger group (three to five years) three single opto-type tests were compared: Sheridan's five and seven letter matching test, the Fooks test and the E card test. The Sheridan test gave the best results and the E card test was found to be unsuitable for this age-group. The Fooks is an attractive test but was less sensitive in the detection of defects than the Sheridan test. In the older group (five to seven years) a single opto-type test, the Sheridan-Gardiner test, was compared with the E chart and the Snellen chart. The Sheridan-Gardiner test was found to have limitations in the detection of defects, including amblyopia, therefore results obtained by this method should not be considered to be directly comparable with those of the Snellen chart. The E chart gave good results in the detection of defects and there was less lateral confusion than had been expected. It has disadvantages, but there is still a place for its use in the screening of normal school-age children. The Snellen chart was the most effective test in the detection of defects, but not all the children were able to co-operate in its use. It is concluded that where it is possible to use the Snellen chart it should always be the method of choice.", "PMID": 976611}
{"id": "pubmed23n0032_10763", "title": "Nocturnal enuresis in childhood.", "content": "Of some 12,000 children in the National Child Development Study for whom the information was available, 10-7 per cent were enuretic between the ages of five and seven years, and 4-8 per cent were enuretic at 11 years. More boys than girls were wet at 11 years, although there was no difference at seven years. At both ages the manual social-classes were over-represented among the children with enuresis. These findings add support to the theory that nocturnal enuresis has multiple causes. The relative importance of social, developmental and psychiatric factors varies with the different groups of children early bed-wetting being associated with delayed development and later enuresis being more strongly associated with behaviour difficulties.", "contents": "Nocturnal enuresis in childhood. Of some 12,000 children in the National Child Development Study for whom the information was available, 10-7 per cent were enuretic between the ages of five and seven years, and 4-8 per cent were enuretic at 11 years. More boys than girls were wet at 11 years, although there was no difference at seven years. At both ages the manual social-classes were over-represented among the children with enuresis. These findings add support to the theory that nocturnal enuresis has multiple causes. The relative importance of social, developmental and psychiatric factors varies with the different groups of children early bed-wetting being associated with delayed development and later enuresis being more strongly associated with behaviour difficulties.", "PMID": 976612}
{"id": "pubmed23n0032_10764", "title": "The behavior of the full-term but underweight newborn infant.", "content": "Ten underweight full-term newborns were compared with 10 full-weight newborns on the Brazelton Neonatal Behavioral Assessment Scale. The Brazelton examination differentiated the two groups clearly on the reflexes of walking, crawling and passive movements of arms and legs, and on rooting and sucking. More importantly, it differentiated the two groups on behaviors which are important for the caretaker of the baby: these are attractiveness, need for stimulation, interactive processes and motor processes. The 10 underweight infants were followed up at a later date during the first year. They showed temperamental organizational difficulties and some indication of psychosomatic reaction to stress. It is possible that the underweight newborn's fragile organization elicits anxiety in the caretaker which makes interaction difficult.", "contents": "The behavior of the full-term but underweight newborn infant. Ten underweight full-term newborns were compared with 10 full-weight newborns on the Brazelton Neonatal Behavioral Assessment Scale. The Brazelton examination differentiated the two groups clearly on the reflexes of walking, crawling and passive movements of arms and legs, and on rooting and sucking. More importantly, it differentiated the two groups on behaviors which are important for the caretaker of the baby: these are attractiveness, need for stimulation, interactive processes and motor processes. The 10 underweight infants were followed up at a later date during the first year. They showed temperamental organizational difficulties and some indication of psychosomatic reaction to stress. It is possible that the underweight newborn's fragile organization elicits anxiety in the caretaker which makes interaction difficult.", "PMID": 976613}
{"id": "pubmed23n0032_10765", "title": "The electroencephalogram of normal adolescent males: visual assessment and relationship with other variables.", "content": "The electroencephalograms (EEGS) were recorded of 223 boys aged from 12 to 16 years inclusive from four different kinds of schools in the former borough of Warley, an industrial area on the outskirts of Birmingham, England. A battery of psychological tests were also administered and descriptions of behaviour were obtained from parents and teachers, as well as information on development and previous medical history. The data were analysed in terms of (a) prevalence of certain EEG features, usually considered abnormal, and (b) the relationship between the EEG and other demographic, developmental and psychological factors. Only 2-2 per cent of the EEGS showed definite abnormalities, such as localised sharp waves and/or spike and wave complexes in their resting record and/or during hyperventilation and photic stimulation, while a further 13-9 per cent showed very slight abnormalities or \"immaturity\". The abnormal and immature EEGS were significantly related to social class, type of school and positive medical history (especially a history of convulsions or faints). Comparisons with previously reported data are discussed.", "contents": "The electroencephalogram of normal adolescent males: visual assessment and relationship with other variables. The electroencephalograms (EEGS) were recorded of 223 boys aged from 12 to 16 years inclusive from four different kinds of schools in the former borough of Warley, an industrial area on the outskirts of Birmingham, England. A battery of psychological tests were also administered and descriptions of behaviour were obtained from parents and teachers, as well as information on development and previous medical history. The data were analysed in terms of (a) prevalence of certain EEG features, usually considered abnormal, and (b) the relationship between the EEG and other demographic, developmental and psychological factors. Only 2-2 per cent of the EEGS showed definite abnormalities, such as localised sharp waves and/or spike and wave complexes in their resting record and/or during hyperventilation and photic stimulation, while a further 13-9 per cent showed very slight abnormalities or \"immaturity\". The abnormal and immature EEGS were significantly related to social class, type of school and positive medical history (especially a history of convulsions or faints). Comparisons with previously reported data are discussed.", "PMID": 976614}
{"id": "pubmed23n0032_10766", "title": "Relationship between changes in somesthetic evoked responses and electroencephalographic findings in the child with hemiplegia.", "content": "The results of somesthetic evoked response (SER) and electroencephalogram (EEG) records in 43 children with hemiplegic cerebral palsy have been compared. In all children there was a positive correlation between the SER changes and the affected side of the body. In 12 children the SER of the \"damaged\" hemisphere disappeared; in 31 children it was less than the response of the \"healthy\" hemisphere. On the other hand, a positive correlation between the EEG changes and the affected side was found in only 16 of the patients, and there was a dubious correlation in three cases. It is concluded that SER data are more accurate and reliable than EEG results. Moreover, SER findings allow cerebral damage to be suspected earlier than the EEG results and therefore are of greater prognostic value.", "contents": "Relationship between changes in somesthetic evoked responses and electroencephalographic findings in the child with hemiplegia. The results of somesthetic evoked response (SER) and electroencephalogram (EEG) records in 43 children with hemiplegic cerebral palsy have been compared. In all children there was a positive correlation between the SER changes and the affected side of the body. In 12 children the SER of the \"damaged\" hemisphere disappeared; in 31 children it was less than the response of the \"healthy\" hemisphere. On the other hand, a positive correlation between the EEG changes and the affected side was found in only 16 of the patients, and there was a dubious correlation in three cases. It is concluded that SER data are more accurate and reliable than EEG results. Moreover, SER findings allow cerebral damage to be suspected earlier than the EEG results and therefore are of greater prognostic value.", "PMID": 976615}
{"id": "pubmed23n0032_10767", "title": "Surgical correction of spastic thumb-in-palm deformity.", "content": "A series of 31 operations on 27 patients for correction of the spastic thumb-in-palm deformity is reported. 19 patients were followed four years or longer post-operatively; the remainder have been followed for at least one year. In approximately 70 per cent of the operations, supplementary procedures were performed on the hand or forearm, as the deformity is usually only one facet of a complex spastic disability of the upper extremity. The surgical technique and illustrative cases are presented. Augmentation of a markedly weak extensor-abductor motor function is necessary, otherwise the spastic thumb-in-palm deformity will recur, as in two cases in the present series which required a second operation. The remainder of the patients were classified as improved, indicating that the thumb was no longer clenched in the palm, could be used in gross grasping activities, and the hand had become more useful. In no instance did the spastic hand become the primary functioning hand post-operatively.", "contents": "Surgical correction of spastic thumb-in-palm deformity. A series of 31 operations on 27 patients for correction of the spastic thumb-in-palm deformity is reported. 19 patients were followed four years or longer post-operatively; the remainder have been followed for at least one year. In approximately 70 per cent of the operations, supplementary procedures were performed on the hand or forearm, as the deformity is usually only one facet of a complex spastic disability of the upper extremity. The surgical technique and illustrative cases are presented. Augmentation of a markedly weak extensor-abductor motor function is necessary, otherwise the spastic thumb-in-palm deformity will recur, as in two cases in the present series which required a second operation. The remainder of the patients were classified as improved, indicating that the thumb was no longer clenched in the palm, could be used in gross grasping activities, and the hand had become more useful. In no instance did the spastic hand become the primary functioning hand post-operatively.", "PMID": 976616}
{"id": "pubmed23n0032_10768", "title": "Platelet mono-amine oxidase activity in the human fetus.", "content": "Measurement of the platelet mono-amine oxidase (MAO) activity in the human fetus of 40 weeks gestation showed it to be equivalent to that found in the adult. The level represents a fully-developed mitochondrial MAO system in platelets and may reflect enzyme maturity in other tissues of the newborn.", "contents": "Platelet mono-amine oxidase activity in the human fetus. Measurement of the platelet mono-amine oxidase (MAO) activity in the human fetus of 40 weeks gestation showed it to be equivalent to that found in the adult. The level represents a fully-developed mitochondrial MAO system in platelets and may reflect enzyme maturity in other tissues of the newborn.", "PMID": 976617}
{"id": "pubmed23n0032_10769", "title": "Neurological disturbance in a phenylketonic child after discontinuation of dietary treatment.", "content": "Signs of moderately severe spastic diplegia appeared in 14-year-old late-treated phenylketonuric boy 13 months after dietary restrictions were lifted. The literature contains reports of untreated phenylketonuric patients developing demyelinating conditions in later childhood. The possible implications of relaxing the diet in early- and late-treated cases of phenylketonuria are discussed.", "contents": "Neurological disturbance in a phenylketonic child after discontinuation of dietary treatment. Signs of moderately severe spastic diplegia appeared in 14-year-old late-treated phenylketonuric boy 13 months after dietary restrictions were lifted. The literature contains reports of untreated phenylketonuric patients developing demyelinating conditions in later childhood. The possible implications of relaxing the diet in early- and late-treated cases of phenylketonuria are discussed.", "PMID": 976618}
{"id": "pubmed23n0032_10770", "title": "Cerebro-hepato-renal syndrome with parental consanguinity.", "content": "A case of cerebro-hepato-renal syndrome with some unusual features is reported. The neuropathological findings are described in detail. Electronmicroscopy showed astrocytes in the demyelinated areas of the brain to contain granules composed of laminated osmiophilic material. These structures could be abnormal mitochondria. The parental consanguinity in this case would further support an autosomal recessive mode of inheritance.", "contents": "Cerebro-hepato-renal syndrome with parental consanguinity. A case of cerebro-hepato-renal syndrome with some unusual features is reported. The neuropathological findings are described in detail. Electronmicroscopy showed astrocytes in the demyelinated areas of the brain to contain granules composed of laminated osmiophilic material. These structures could be abnormal mitochondria. The parental consanguinity in this case would further support an autosomal recessive mode of inheritance.", "PMID": 976619}
{"id": "pubmed23n0032_10771", "title": "Effect of calcium infusions of serum calcium and gastric acid secretion.", "content": "37 patients were studied with calcium infusions. Of these, 20 had previously undergone truncal vagotomy and pyloroplasty for duodenal ulcer disease, and 17 were unoperated patients with duodenal ulcer disease. Calcium was given intravenously either at a dose of 5 mg/kg/h for 3 h, or 4 mg/kg/h for 4 h. Gastric juice was collected by continuous suction. Results showed the 3-hour infusion raised calcium more than the 4-hour infusion. Top serum calcium achieved, however, did not correlate with calcium-stimulated gastric acid output, either with or without vagotomy. Stimulated gastric acid secretion was markedly less with vagotomy than without. It is suggested that the level of gastric acid stimulated by infusions might discriminate complete, from incomplete, vagotomies better than insulin, and that the 4-hour infusion is safer.", "contents": "Effect of calcium infusions of serum calcium and gastric acid secretion. 37 patients were studied with calcium infusions. Of these, 20 had previously undergone truncal vagotomy and pyloroplasty for duodenal ulcer disease, and 17 were unoperated patients with duodenal ulcer disease. Calcium was given intravenously either at a dose of 5 mg/kg/h for 3 h, or 4 mg/kg/h for 4 h. Gastric juice was collected by continuous suction. Results showed the 3-hour infusion raised calcium more than the 4-hour infusion. Top serum calcium achieved, however, did not correlate with calcium-stimulated gastric acid output, either with or without vagotomy. Stimulated gastric acid secretion was markedly less with vagotomy than without. It is suggested that the level of gastric acid stimulated by infusions might discriminate complete, from incomplete, vagotomies better than insulin, and that the 4-hour infusion is safer.", "PMID": 976622}
{"id": "pubmed23n0032_10772", "title": "The effect of phenobarbital on patients with Dubin-Johnson syndrome.", "content": "11 patients with jaundice of Dubin-Johnson type were treated for 2 weeks with phenobarbital. Serum bilirubin diminished and hepatic clearance of sulfobromophthalein was variably enhanced; other measures of hepatic function were not significantly changed during therapy.", "contents": "The effect of phenobarbital on patients with Dubin-Johnson syndrome. 11 patients with jaundice of Dubin-Johnson type were treated for 2 weeks with phenobarbital. Serum bilirubin diminished and hepatic clearance of sulfobromophthalein was variably enhanced; other measures of hepatic function were not significantly changed during therapy.", "PMID": 976623}
{"id": "pubmed23n0032_10773", "title": "Risk factors in ulcerative colitis.", "content": "In 504 Jewish patients with ulcerative colitis the following risk factors were evaluated: sex, age at onset of disease, community group, extent of disease, and duration of disease. The disease was more severe in females. Severe attacks, weight loss, iron deficiency, liver disease and arthritis were significantly more frequent in females than in mles. Mortality was higher in patients who contracted the disease above age 50. The disease appeared to be more severe in patiets of Ashkenazi origin as compared to Orientals. The difference failed to reach statistical signifcance except for mortality. We confirmed the well-known adverse effect of extensive colonic involvement. Severe attacks, a severe course of the disease and extensive colonic involvement are more frequent with increasing duration of the disease.", "contents": "Risk factors in ulcerative colitis. In 504 Jewish patients with ulcerative colitis the following risk factors were evaluated: sex, age at onset of disease, community group, extent of disease, and duration of disease. The disease was more severe in females. Severe attacks, weight loss, iron deficiency, liver disease and arthritis were significantly more frequent in females than in mles. Mortality was higher in patients who contracted the disease above age 50. The disease appeared to be more severe in patiets of Ashkenazi origin as compared to Orientals. The difference failed to reach statistical signifcance except for mortality. We confirmed the well-known adverse effect of extensive colonic involvement. Severe attacks, a severe course of the disease and extensive colonic involvement are more frequent with increasing duration of the disease.", "PMID": 976624}
{"id": "pubmed23n0032_10774", "title": "Action of cholecystokinin and caerulein on the rabbit sphincter of Oddi.", "content": "The action of cholecystokinin (CCK) and caerulein upon the rabbit sphincter of Oddi was investigated by electromyography, manometry and measuring of the flow. Intravenous injection of CCK resulted in a marked rise of the sphincter activity with a maximum at the 1st min and return to the basal level at the 10th min. Injection of increasing doses of hormone showed that the effect was proportional to the logarithm of the dose. The same results were obtained with caerulein. Parallely, CCK provoked a rise of the biliary pressure also proportional to the logarithm of the dose and a reduction to the biliary flow. The discrepancies between these results and some other experiments are discussed. It is concluded that, in the rabbit, CCK increases the activity of the sphincter of Oddi.", "contents": "Action of cholecystokinin and caerulein on the rabbit sphincter of Oddi. The action of cholecystokinin (CCK) and caerulein upon the rabbit sphincter of Oddi was investigated by electromyography, manometry and measuring of the flow. Intravenous injection of CCK resulted in a marked rise of the sphincter activity with a maximum at the 1st min and return to the basal level at the 10th min. Injection of increasing doses of hormone showed that the effect was proportional to the logarithm of the dose. The same results were obtained with caerulein. Parallely, CCK provoked a rise of the biliary pressure also proportional to the logarithm of the dose and a reduction to the biliary flow. The discrepancies between these results and some other experiments are discussed. It is concluded that, in the rabbit, CCK increases the activity of the sphincter of Oddi.", "PMID": 976625}
{"id": "pubmed23n0032_10775", "title": "Experimental colitis.", "content": "Ulcerative colitis and Crohn's disease are complex, problematic diseases of unknown etiology in man, and appropriate experimental models would be useful in elucidating their pathogenesis and treatment. Although there have been numerous attempts to produce inflammatory ulcerative colonic disease in laboratory animals resembling those human disease forms, none has been entirely successful. Investigators have conducted experiments involving almost every etiological factor suggested for initiation of these diseases. The methods reviewed in this paper include production of experimental colitis by vascular impairment, and immunological methods such as bacterial infection, allergic reactions, direct and indirect hypersensitivity reactions, as well as autoimmune mechanisms. The results of carrageenan-induced colitis, irradiation, dietary, and drug-induced techniques are also discussed and the frequency and nature of spontaneous colonic lesions in animals is summarized.", "contents": "Experimental colitis. Ulcerative colitis and Crohn's disease are complex, problematic diseases of unknown etiology in man, and appropriate experimental models would be useful in elucidating their pathogenesis and treatment. Although there have been numerous attempts to produce inflammatory ulcerative colonic disease in laboratory animals resembling those human disease forms, none has been entirely successful. Investigators have conducted experiments involving almost every etiological factor suggested for initiation of these diseases. The methods reviewed in this paper include production of experimental colitis by vascular impairment, and immunological methods such as bacterial infection, allergic reactions, direct and indirect hypersensitivity reactions, as well as autoimmune mechanisms. The results of carrageenan-induced colitis, irradiation, dietary, and drug-induced techniques are also discussed and the frequency and nature of spontaneous colonic lesions in animals is summarized.", "PMID": 976626}
{"id": "pubmed23n0032_10776", "title": "Water filtration of the forearm in short- and long-term diabetes mellitus.", "content": "Blood flow and capillary filtration coefficient (CFC) were measured by strain-gauge plethysmography on the upper and lower third of the forearm in 9 normal subjects and 29 well regulated patients with diabetes mellitus of varying duration (less than 10 years, 10 to 20 years, and more than 20 years). There was no difference in blood flow in the four groups, but CFC was significantly increased in long-term diabetes (duration above 20 years) when measured at the distal part of the forearm near the wrist. Calculations showed that this was probably due to the relatively high contribution of connective tissue in this part of the forearm. Increased water filtration in connective tissue in long-term diabetics is in accordance with earlier findings of a lowered subcutaneous interstitial fluid albumin concentration in long-term diabetics, this being explained by an increase in net water outflux from the microcirculation.", "contents": "Water filtration of the forearm in short- and long-term diabetes mellitus. Blood flow and capillary filtration coefficient (CFC) were measured by strain-gauge plethysmography on the upper and lower third of the forearm in 9 normal subjects and 29 well regulated patients with diabetes mellitus of varying duration (less than 10 years, 10 to 20 years, and more than 20 years). There was no difference in blood flow in the four groups, but CFC was significantly increased in long-term diabetes (duration above 20 years) when measured at the distal part of the forearm near the wrist. Calculations showed that this was probably due to the relatively high contribution of connective tissue in this part of the forearm. Increased water filtration in connective tissue in long-term diabetics is in accordance with earlier findings of a lowered subcutaneous interstitial fluid albumin concentration in long-term diabetics, this being explained by an increase in net water outflux from the microcirculation.", "PMID": 976633}
{"id": "pubmed23n0032_10777", "title": "Insulin secretion and biosynthesis in sucrose fed rats.", "content": "Long term feeding of a sucrose rich diet to rats is accompanied by a decreased glucose assimilation rate, despite high plasma insulin levels. Hyperinsulinism is at least partially based on a relative obesity, with increased amounts of abdominal- and retroperitoneal fat tissue, but unchanged total body weight compared to starch fed controls. The secretory pattern of insulin release was studied following glucose, arginine, fructose and sulfonylurea administration in the isolated perfused pancreas of sucrose and isocaloric starch fed rats. In addition, isolated islets of Langerhans were used to demonstrate the effects of glucose on insulin secretion and the incorporation of H-3 leucine into the proinsulin and insulin fraction of islet proteins. Following 11 mM glucose, the dynamics of insulin release in the isolated perfused pancreas of sucrose fed rats is characterized by a markedly elevated, late plateau-like response, usually seen only at higher glucose concentrations. Hyperinsulinism, as compared to starch fed controls, can also be demonstrated following arginine and the sulfonylurea HB-419, whereas fructose has no effect in the presence of low glucose concentrations. During incubation of the pancreatic islets, the hyperinsulinism in sucrose-, compared to starch fed rats, is more pronounced at 11 mM glucose than at 5.5 mM glucose. The incorporation of H-3 leucine into the proinsulin-insulin fraction of islet proteins in sucrose compared to starch fed rats, however, is significantly greater with glucose 5.5 mM than at high glucose level. In sucrose fed rats, secretion and biosynthesis of insulin thus appear to be elevated but closely linked only at physiological glucose concentration.", "contents": "Insulin secretion and biosynthesis in sucrose fed rats. Long term feeding of a sucrose rich diet to rats is accompanied by a decreased glucose assimilation rate, despite high plasma insulin levels. Hyperinsulinism is at least partially based on a relative obesity, with increased amounts of abdominal- and retroperitoneal fat tissue, but unchanged total body weight compared to starch fed controls. The secretory pattern of insulin release was studied following glucose, arginine, fructose and sulfonylurea administration in the isolated perfused pancreas of sucrose and isocaloric starch fed rats. In addition, isolated islets of Langerhans were used to demonstrate the effects of glucose on insulin secretion and the incorporation of H-3 leucine into the proinsulin and insulin fraction of islet proteins. Following 11 mM glucose, the dynamics of insulin release in the isolated perfused pancreas of sucrose fed rats is characterized by a markedly elevated, late plateau-like response, usually seen only at higher glucose concentrations. Hyperinsulinism, as compared to starch fed controls, can also be demonstrated following arginine and the sulfonylurea HB-419, whereas fructose has no effect in the presence of low glucose concentrations. During incubation of the pancreatic islets, the hyperinsulinism in sucrose-, compared to starch fed rats, is more pronounced at 11 mM glucose than at 5.5 mM glucose. The incorporation of H-3 leucine into the proinsulin-insulin fraction of islet proteins in sucrose compared to starch fed rats, however, is significantly greater with glucose 5.5 mM than at high glucose level. In sucrose fed rats, secretion and biosynthesis of insulin thus appear to be elevated but closely linked only at physiological glucose concentration.", "PMID": 976634}
{"id": "pubmed23n0032_10778", "title": "Effect of somatostatin on metabolic and hormonal changes induced by nicotinic acid in insulin-dependent diabetics.", "content": "The study investigated the respective influences of nicotinic acid and somatostatin on plasma concentrations of blood glucose, free fatty acids, glucagon, growth hormone and cortisol in insulin-dependent diabetic subjects. After administration of nicotinic acid alone, marked depression of plasma FFA was accompanied by significant increases of plasma glucagon, growth hormone and cortisol. The glucagon and growth hormone responses to nicotinic acid were significantly reduced when plasma FFA were raised by intravenous administration of heparin and triglycerides. Somatostatin alone induced a significant decrease in blood glucose, plasma glucagon and growth hormone concentrations. Plasma FFA remained unchanged. Somatostatin did not modify the nicotinic acid-induced fall in plasma FFA, but completely blocked the corresponding increments in glucagon and growth hormone. The cortisol rise was not altered by somatostatin. Rebound of glucagon and growth hormone levels were seen upon discontinuation of the somatostatin administration. These results demonstrate that the plasma FFA concentration plays a role in the regulation of glucagon and growth hormone secretion in insulin-dependent diabetics. Furthermore, they indicate that somatostatin, previously shown to be capable of negating the stimulatory effect of various factors on glucagon and growth hormone secretion, also affects the response of these hormones to FFA depression.", "contents": "Effect of somatostatin on metabolic and hormonal changes induced by nicotinic acid in insulin-dependent diabetics. The study investigated the respective influences of nicotinic acid and somatostatin on plasma concentrations of blood glucose, free fatty acids, glucagon, growth hormone and cortisol in insulin-dependent diabetic subjects. After administration of nicotinic acid alone, marked depression of plasma FFA was accompanied by significant increases of plasma glucagon, growth hormone and cortisol. The glucagon and growth hormone responses to nicotinic acid were significantly reduced when plasma FFA were raised by intravenous administration of heparin and triglycerides. Somatostatin alone induced a significant decrease in blood glucose, plasma glucagon and growth hormone concentrations. Plasma FFA remained unchanged. Somatostatin did not modify the nicotinic acid-induced fall in plasma FFA, but completely blocked the corresponding increments in glucagon and growth hormone. The cortisol rise was not altered by somatostatin. Rebound of glucagon and growth hormone levels were seen upon discontinuation of the somatostatin administration. These results demonstrate that the plasma FFA concentration plays a role in the regulation of glucagon and growth hormone secretion in insulin-dependent diabetics. Furthermore, they indicate that somatostatin, previously shown to be capable of negating the stimulatory effect of various factors on glucagon and growth hormone secretion, also affects the response of these hormones to FFA depression.", "PMID": 976635}
{"id": "pubmed23n0032_10779", "title": "Plasma concentrations of unconjugated estrone, estradiol-17beta and estriol, and HCS throughout pregnancy in diabetics and gestational diabetics.", "content": "Plasma unconjugated estrone (E1), estradiol-17beta (E2) and estriol (E3), and HCS were measured in the same plasma samples collected throughout pregnancy in 19 gestational diabetics (GD) and 21 diabetics (D). When compared to the results obtained in 22 normal subjects, plasma levels of E1 and E2 were significantly elevated in D in the second half of gestation. The results were intermediate although closer to the normals, in GD. E3 values were not different from the normals in both D and GD. HCS values were lower than normal in early pregnancy in both D and GD. In late pregnancy HCS levels were not different from normal in either D or GD, although some individual values were much above the upper limit in some diabetic patients. The hormonal ratios in D and GD parallel those in normals, although E3/E2 and HCS/E2 were lower in D. These results are discussed with respect to the different behaviour of E2 and E3, taking into account the difference in their respective biosynthetic pathways. Besides a possible quantitative modification of the placental function in D, the results could tentatively be explained by a qualitative change in the fetal estrogen precursors to placental aromatization, in favour of the 16 non-hydroxylated compound. However, maternal modifications in precursor production or in estrogen metabolism can be an alternative hypothesis. Finally, the present work does not support the hypothetical estrogen deficiency in diabetic pregnancy. Estrogen treatment appears to have no objective justification.", "contents": "Plasma concentrations of unconjugated estrone, estradiol-17beta and estriol, and HCS throughout pregnancy in diabetics and gestational diabetics. Plasma unconjugated estrone (E1), estradiol-17beta (E2) and estriol (E3), and HCS were measured in the same plasma samples collected throughout pregnancy in 19 gestational diabetics (GD) and 21 diabetics (D). When compared to the results obtained in 22 normal subjects, plasma levels of E1 and E2 were significantly elevated in D in the second half of gestation. The results were intermediate although closer to the normals, in GD. E3 values were not different from the normals in both D and GD. HCS values were lower than normal in early pregnancy in both D and GD. In late pregnancy HCS levels were not different from normal in either D or GD, although some individual values were much above the upper limit in some diabetic patients. The hormonal ratios in D and GD parallel those in normals, although E3/E2 and HCS/E2 were lower in D. These results are discussed with respect to the different behaviour of E2 and E3, taking into account the difference in their respective biosynthetic pathways. Besides a possible quantitative modification of the placental function in D, the results could tentatively be explained by a qualitative change in the fetal estrogen precursors to placental aromatization, in favour of the 16 non-hydroxylated compound. However, maternal modifications in precursor production or in estrogen metabolism can be an alternative hypothesis. Finally, the present work does not support the hypothetical estrogen deficiency in diabetic pregnancy. Estrogen treatment appears to have no objective justification.", "PMID": 976636}
{"id": "pubmed23n0032_10780", "title": "Quantitative evaluation of somatotrophic cells (SC) in the adenohypophysis of normal and diabetic male Chinese hamsters.", "content": "The pituitary of genetically diabetic Chinese hamsters contains significantly (p less than 0.001) greater numbers of somatotrophes than pituitary of non-diabetics. This may play a role in the aetiopathology of diabetes in this species or at least contribute to the maintenance of hyperglycaemia.", "contents": "Quantitative evaluation of somatotrophic cells (SC) in the adenohypophysis of normal and diabetic male Chinese hamsters. The pituitary of genetically diabetic Chinese hamsters contains significantly (p less than 0.001) greater numbers of somatotrophes than pituitary of non-diabetics. This may play a role in the aetiopathology of diabetes in this species or at least contribute to the maintenance of hyperglycaemia.", "PMID": 976637}
{"id": "pubmed23n0032_10781", "title": "Human C-peptide immunoreactivity (CPR) in blood and urine - evaluation of a radioimmunoassay method and its clinical applications.", "content": "A double-antibody radioimmunoassay method, using synthetic human connecting peptide as an immunizing antigen and standard, was evaluated for clinical assay of blood and urine samples. Normal fasting blood connecting peptide immunoreacivity (CPR) was 2.45 +/- 0.96 ng/ml, increasing promptly after a 50 g oral glucose load, but somewhat slower than insulin. Molar concentration of CPR exceeded that of insulin. CPR responses to glucose were subnormal in diabetics, very low in juvenile-type cases, and often poor in patients on insulin treatment. Fasting CPR levels were elevated in patients on corticosteroid treatment and with uraemia. A patient with insulin \"auto-antibody\" had high serum CPR. A considerable amount of CPR appeared in urine. Normal daily excretion of CPR was 1.52 +/- 0.55 mug/kg or 55.1 +/- 18.2 ng/mg creatinine. Urine CPR was very low in juvenile-type diabetics, and elevated in patients on corticosteroid treatment. The results confirm that blood and urine CPR are useful measures of the endocrine pancreatic function.", "contents": "Human C-peptide immunoreactivity (CPR) in blood and urine - evaluation of a radioimmunoassay method and its clinical applications. A double-antibody radioimmunoassay method, using synthetic human connecting peptide as an immunizing antigen and standard, was evaluated for clinical assay of blood and urine samples. Normal fasting blood connecting peptide immunoreacivity (CPR) was 2.45 +/- 0.96 ng/ml, increasing promptly after a 50 g oral glucose load, but somewhat slower than insulin. Molar concentration of CPR exceeded that of insulin. CPR responses to glucose were subnormal in diabetics, very low in juvenile-type cases, and often poor in patients on insulin treatment. Fasting CPR levels were elevated in patients on corticosteroid treatment and with uraemia. A patient with insulin \"auto-antibody\" had high serum CPR. A considerable amount of CPR appeared in urine. Normal daily excretion of CPR was 1.52 +/- 0.55 mug/kg or 55.1 +/- 18.2 ng/mg creatinine. Urine CPR was very low in juvenile-type diabetics, and elevated in patients on corticosteroid treatment. The results confirm that blood and urine CPR are useful measures of the endocrine pancreatic function.", "PMID": 976638}
{"id": "pubmed23n0032_10782", "title": "Disappearance rate of endogenous human C-peptide from blood.", "content": "Glucagon (1 mg) and glucose (60 ml of 50% solution) were infused over 60 min to three normal and one obese subjects and two insulinoma patients. Plasma C-peptide immunoreactivity (CPR) and immunoreactive insulin (IRI) increased during the infusion. Half time of CPR after cessation of the infusion was 20.1 +/- 4.0 min, and that of IRI 9.8 +/- 1.3 min, respectively. This difference partly explains the higher molar concentration in plasma of CPR than IRI.", "contents": "Disappearance rate of endogenous human C-peptide from blood. Glucagon (1 mg) and glucose (60 ml of 50% solution) were infused over 60 min to three normal and one obese subjects and two insulinoma patients. Plasma C-peptide immunoreactivity (CPR) and immunoreactive insulin (IRI) increased during the infusion. Half time of CPR after cessation of the infusion was 20.1 +/- 4.0 min, and that of IRI 9.8 +/- 1.3 min, respectively. This difference partly explains the higher molar concentration in plasma of CPR than IRI.", "PMID": 976639}
{"id": "pubmed23n0032_10783", "title": "The excretion of insulin in urine.", "content": "The concentration of biologically active insulin was measured by the isolated fat cell method in serum and urine from healthy subjects and compared with the concentration of immunoreactive insulin. In both urine and serum the values obtained by the two methods correlated closely; In addition, there was a close correlation between the concentration of biologically active and immunoreactive insulin in urine from maturity onset diabetics. Therefore, conclusions on the excretion in the urine and the urinary clearance of insulin, which are based on measurements of immunoreactive insulin, are also valid for biologically active insulin.", "contents": "The excretion of insulin in urine. The concentration of biologically active insulin was measured by the isolated fat cell method in serum and urine from healthy subjects and compared with the concentration of immunoreactive insulin. In both urine and serum the values obtained by the two methods correlated closely; In addition, there was a close correlation between the concentration of biologically active and immunoreactive insulin in urine from maturity onset diabetics. Therefore, conclusions on the excretion in the urine and the urinary clearance of insulin, which are based on measurements of immunoreactive insulin, are also valid for biologically active insulin.", "PMID": 976640}
{"id": "pubmed23n0032_10784", "title": "The role of calcium in glucagon release, interactions between glucose and calcium.", "content": "The interrelationships between glucose and calcium in glucagon release were investigated using the dynamic system of the in vitro perfused rat pancreas. When calcium deprivation was induced in the presence of fixed concentrations of glucose prevailing throughout the experiments (3.3, 5.5, 8.3 and 16.6 mM), an enhancement of glucagon release invariably occurred, the shape and amplitude of such response differing in relation to the environmental glucose concentration. Such enhancement of glucagon release was readily reversible upon restoration of normal calcium levels. By contrast, during the period of calcium deprivation itself, glucagon release was little influenced by either raised (from 3.3 to 16.6 mM) or decreased (from 16.6 to 3.3 mM) glucose concentrations. These results clearly indicate that calcium plays, at least, a dual role - both inhibitory and permissive - in glucagon secretion, but the intimate mechanisms by which calcium exerts such a dual action are at present unknown.", "contents": "The role of calcium in glucagon release, interactions between glucose and calcium. The interrelationships between glucose and calcium in glucagon release were investigated using the dynamic system of the in vitro perfused rat pancreas. When calcium deprivation was induced in the presence of fixed concentrations of glucose prevailing throughout the experiments (3.3, 5.5, 8.3 and 16.6 mM), an enhancement of glucagon release invariably occurred, the shape and amplitude of such response differing in relation to the environmental glucose concentration. Such enhancement of glucagon release was readily reversible upon restoration of normal calcium levels. By contrast, during the period of calcium deprivation itself, glucagon release was little influenced by either raised (from 3.3 to 16.6 mM) or decreased (from 16.6 to 3.3 mM) glucose concentrations. These results clearly indicate that calcium plays, at least, a dual role - both inhibitory and permissive - in glucagon secretion, but the intimate mechanisms by which calcium exerts such a dual action are at present unknown.", "PMID": 976641}
{"id": "pubmed23n0032_10785", "title": "Neural induction by previously induced epiblast in avian embryo in vitro.", "content": "Pieces of previously neurally induced and competent epiblast of chick and, respectively, quail primitive streak blastoderms were cultured in close contact with each other for 48 h. In several cases, both pieces differentiated into neural direction, which indicates the occurrence of a homoiogenetic induction. There was a considerable mixing of cells of different origin, especially in the undifferentiated controls. In general, the dorsoventral orientation of the previously induced epiblast was retained, but the orientation of the competent epiblast cells was more flexible and could be influenced by the neighbouring neuralised cells.", "contents": "Neural induction by previously induced epiblast in avian embryo in vitro. Pieces of previously neurally induced and competent epiblast of chick and, respectively, quail primitive streak blastoderms were cultured in close contact with each other for 48 h. In several cases, both pieces differentiated into neural direction, which indicates the occurrence of a homoiogenetic induction. There was a considerable mixing of cells of different origin, especially in the undifferentiated controls. In general, the dorsoventral orientation of the previously induced epiblast was retained, but the orientation of the competent epiblast cells was more flexible and could be influenced by the neighbouring neuralised cells.", "PMID": 976642}
{"id": "pubmed23n0032_10786", "title": "In vitro transcription of partially deproteinised and reconstituted chromatins from pigeon erythroid cells.", "content": "The role of protein fractions extracted from chromatin preparations with NaCl concentrations up to 0.7 M in the additional repression of pigeon erythrocyte genome was investigated. The chromatins from erythroblasts and erythrocytes were dissociated with 0.7 M NaCl and reconstituted from dissociated components to obtain the \"original\" and \"hybrid\" chromatins. The protein fraction extracted from erythrocyte chromatin at 0.7 M NaCl was more efficient in the restriction of transcription with partially deproteinised chromatin from both erythroblasts and erythrocytes. The partially deproteinised chromatin from both erythorblasts and erythrocytes. The partially deproteinised chromatins were also complexed in various conditions with purified F1 or F2c histones. In such complexes, F2c histone was a stronger inhibitor of template activity than histone F1.", "contents": "In vitro transcription of partially deproteinised and reconstituted chromatins from pigeon erythroid cells. The role of protein fractions extracted from chromatin preparations with NaCl concentrations up to 0.7 M in the additional repression of pigeon erythrocyte genome was investigated. The chromatins from erythroblasts and erythrocytes were dissociated with 0.7 M NaCl and reconstituted from dissociated components to obtain the \"original\" and \"hybrid\" chromatins. The protein fraction extracted from erythrocyte chromatin at 0.7 M NaCl was more efficient in the restriction of transcription with partially deproteinised chromatin from both erythroblasts and erythrocytes. The partially deproteinised chromatin from both erythorblasts and erythrocytes. The partially deproteinised chromatins were also complexed in various conditions with purified F1 or F2c histones. In such complexes, F2c histone was a stronger inhibitor of template activity than histone F1.", "PMID": 976643}
{"id": "pubmed23n0032_10787", "title": "On the mode of activation of sequestered messengers in Artemia salina.", "content": "Activation of the dormant embryos of Artemia salina was marked by a rapid increase in 32P uptake which reached a stationary phase after 6 h of activation. The increase in 32P uptake by whole cysts was paralleled by its incorporation into nucleotides. Fractionation of acid-soluble nucleotides and alkaline hydrolysate of nucleic acids on Dowex-1-formate column revealed the 32P radioactivity to be exclusively localised in AMP. Analysis of the labelled RNA species extracted at different stages of development indicated a preferential labelling of small molecular weight species till the emergence of the embryos, followed by the de novo synthesis of messenger and stable RNA species in later stages of development. During early development, polyadenylated RNA species were localised in the particulate fraction sedimenting at 16,000 rpm and their location shifted to the soluble fraction as development proceeded. Activation of performed messengers by phosphorylation of the adenylate residue of their poly A stretches and translocation of the capacitated messengers to the cytosol via a RNP-membrane complex is proposed as a trigger of embryonic differentiation.", "contents": "On the mode of activation of sequestered messengers in Artemia salina. Activation of the dormant embryos of Artemia salina was marked by a rapid increase in 32P uptake which reached a stationary phase after 6 h of activation. The increase in 32P uptake by whole cysts was paralleled by its incorporation into nucleotides. Fractionation of acid-soluble nucleotides and alkaline hydrolysate of nucleic acids on Dowex-1-formate column revealed the 32P radioactivity to be exclusively localised in AMP. Analysis of the labelled RNA species extracted at different stages of development indicated a preferential labelling of small molecular weight species till the emergence of the embryos, followed by the de novo synthesis of messenger and stable RNA species in later stages of development. During early development, polyadenylated RNA species were localised in the particulate fraction sedimenting at 16,000 rpm and their location shifted to the soluble fraction as development proceeded. Activation of performed messengers by phosphorylation of the adenylate residue of their poly A stretches and translocation of the capacitated messengers to the cytosol via a RNP-membrane complex is proposed as a trigger of embryonic differentiation.", "PMID": 976644}
{"id": "pubmed23n0032_10788", "title": "Evidence for chromosome endoreduplication in Eudorina californica, a colonial alga.", "content": "Several nuclear events seen during the cleavage period in Eudorina suggest that chromosome endoreduplication, proportional to the number of cells to be produced, may occur in the gonidia prior to cleavage. Presumably the DNA concentration is reduced to the haploid level during rapid, successive divisions of the cleavage period. To test this hypothesis, I determined DNA content of gonidia as they grew from 4 mum to 38 mum in diameter between cleavage periods. During growth from 4 mum to 8 mum in diameter, the DNA concentration remained at the haploid level of 0.17 pg/cell. As gonidia in 64 cell colonies continued to grow from 8 mum to 33 mum in diameter, their DNA concentrations increased 60-fold. Analysis of the Eudorina DNA by equilibrium centrifugation in CsCl showed only 2 bands with buoyant densities of 1.721 g/cm3 and 1.699 g/cm3, presumed to be nuclear and chloroplast, respectively, on the basis of labelling with 3H-thymidine and 3H-adenine. The 8:2 ratio of the two bands did not change with increase in cell size and no other bands were detected, suggesting that both nuclear and chloroplast DNAs were synthesised proportionately prior to the cleavage period.", "contents": "Evidence for chromosome endoreduplication in Eudorina californica, a colonial alga. Several nuclear events seen during the cleavage period in Eudorina suggest that chromosome endoreduplication, proportional to the number of cells to be produced, may occur in the gonidia prior to cleavage. Presumably the DNA concentration is reduced to the haploid level during rapid, successive divisions of the cleavage period. To test this hypothesis, I determined DNA content of gonidia as they grew from 4 mum to 38 mum in diameter between cleavage periods. During growth from 4 mum to 8 mum in diameter, the DNA concentration remained at the haploid level of 0.17 pg/cell. As gonidia in 64 cell colonies continued to grow from 8 mum to 33 mum in diameter, their DNA concentrations increased 60-fold. Analysis of the Eudorina DNA by equilibrium centrifugation in CsCl showed only 2 bands with buoyant densities of 1.721 g/cm3 and 1.699 g/cm3, presumed to be nuclear and chloroplast, respectively, on the basis of labelling with 3H-thymidine and 3H-adenine. The 8:2 ratio of the two bands did not change with increase in cell size and no other bands were detected, suggesting that both nuclear and chloroplast DNAs were synthesised proportionately prior to the cleavage period.", "PMID": 976645}
{"id": "pubmed23n0032_10789", "title": "A model implicating the Langerhans cell in keratinocyte proliferation control.", "content": "A tentative model is presented which is based on existing data and our own cell kinetic and morphological observations in mice. The model suggests that the epidermal Langerhans cell plays a role in proliferation control of keratinocytes and may also act as an epidermal stem cell.", "contents": "A model implicating the Langerhans cell in keratinocyte proliferation control. A tentative model is presented which is based on existing data and our own cell kinetic and morphological observations in mice. The model suggests that the epidermal Langerhans cell plays a role in proliferation control of keratinocytes and may also act as an epidermal stem cell.", "PMID": 976646}
{"id": "pubmed23n0032_10790", "title": "DNA synthesis by hybrid echinoid embryos produced from parent species of low and high temperature tolerance.", "content": "Synthesis of DNA, as measured by quantitative Feulgen microspectrophotometry, was studied in reciprocal echinoid hybrids and their homospermic controls. Both parent species develop at the same rate until hatching, at 20 degrees C. One parent (Lytechinus) will develop normally at 25 degrees C, a temperature lethal for the other parent employed in the studies (Strongylocentrotus). Strongylocentrotus will develop normally at 10 degrees C, a temperature at which Lytechinus fertilised eggs live, but fail to cleave. It was found that sperm of Lytechinus in eggs of Strongylocentrotus (SL hybrid) show a slowing of the S phase of the cell cycle at 10 degrees C, and that sperm of Strongylocentrotus in eggs of Lytechinus (LS hybrid) show a slowing of the S phase at 25 degrees C. These slowing effects are not noted during cleavage stages. The results are discussed in terms of proteins produced during the time of active transcription of the hybrid genome which may be rate-limiting in the synthesis of DNA in the hybrid embryos under these temperature extremes.", "contents": "DNA synthesis by hybrid echinoid embryos produced from parent species of low and high temperature tolerance. Synthesis of DNA, as measured by quantitative Feulgen microspectrophotometry, was studied in reciprocal echinoid hybrids and their homospermic controls. Both parent species develop at the same rate until hatching, at 20 degrees C. One parent (Lytechinus) will develop normally at 25 degrees C, a temperature lethal for the other parent employed in the studies (Strongylocentrotus). Strongylocentrotus will develop normally at 10 degrees C, a temperature at which Lytechinus fertilised eggs live, but fail to cleave. It was found that sperm of Lytechinus in eggs of Strongylocentrotus (SL hybrid) show a slowing of the S phase of the cell cycle at 10 degrees C, and that sperm of Strongylocentrotus in eggs of Lytechinus (LS hybrid) show a slowing of the S phase at 25 degrees C. These slowing effects are not noted during cleavage stages. The results are discussed in terms of proteins produced during the time of active transcription of the hybrid genome which may be rate-limiting in the synthesis of DNA in the hybrid embryos under these temperature extremes.", "PMID": 976647}
{"id": "pubmed23n0032_10791", "title": "On the origin of primordial germ cells in the chick embryo.", "content": "An attempt was made to re-examine the location of the primordial germ cells (PGCs) in very young chick embryos. Freshly laid blastoderms, prior to hypoblast formation, of a known anterio-posterior axis, were transversely bisected and each half was separately grown in vitro. Both anterior and posterior halves were shown to be fertile and each was shown to contain roughly the same amount of PGCs as a normal control embryo. It has been concluded that in the chick as well as in the duck there is no concentration of cells containing germinal plasm in the posterior part of the blastoderm. Two other possibilities should be investigated: 1. A concentric arrangement of cells containing germinal plasm. 2. The absence of a germinal plasm and a relatively late appearance of PGCs as a result of induction.", "contents": "On the origin of primordial germ cells in the chick embryo. An attempt was made to re-examine the location of the primordial germ cells (PGCs) in very young chick embryos. Freshly laid blastoderms, prior to hypoblast formation, of a known anterio-posterior axis, were transversely bisected and each half was separately grown in vitro. Both anterior and posterior halves were shown to be fertile and each was shown to contain roughly the same amount of PGCs as a normal control embryo. It has been concluded that in the chick as well as in the duck there is no concentration of cells containing germinal plasm in the posterior part of the blastoderm. Two other possibilities should be investigated: 1. A concentric arrangement of cells containing germinal plasm. 2. The absence of a germinal plasm and a relatively late appearance of PGCs as a result of induction.", "PMID": 976648}
{"id": "pubmed23n0032_10792", "title": "Foetal rat pancreas in organ culture. Effect of corticosterone concentrations on the acinar and islet cell components.", "content": "Foetal rat pancreatic explants (20-day postcoitum) were grown in organ culture on medium enriched with serum and embryo extract containing various concentrations of corticosterone. Normal pancreatic exocrine morphology was preserved. In addition, media amylase concentration remained high and media insulin was suppressed. This is in sharp contrast to explants incubated on control medium without addition of steroid in which a rapid dissappearance of the acinar component and a selective proliferation of the islet cells was noted. The magnitude of these effects was related to the concentration of the steroid. Corticosterone was effective in preserving pancreatic acinar cells through 8-10 days in vitro. Removal of high levels of corticosterone from the incubation medium after 4 days of culture resulted in a decrease in media amylase and a fall in explant acinar cell mass. The media insulin returned to control levels during the following 4 days of culture. Addition of corticosterone to the media following 4 days of control culture resulted in no increase in media amylase and no statistically significant differences in explant acinar cell mass. Media insulin was decreased from control levels following the additional 4 days of incubation. However, corticosterone, even at a concentration of 10.0 mug/ml, was not effective in depressing insulin secretion as was foetal adrenal co-culture. It is proposed that adrenal corticosteroids are responsible for the maintenance of differentiated acinar cells previously observed in pancreatic adrenal co-culture. This suggests that corticosteroids may play an important role in in vivo pancreatic morphogenesis and cytodifferentiation. In addition, adrenal corticosteroids directly inhibit insulin release from the explant beta cells in vitro.", "contents": "Foetal rat pancreas in organ culture. Effect of corticosterone concentrations on the acinar and islet cell components. Foetal rat pancreatic explants (20-day postcoitum) were grown in organ culture on medium enriched with serum and embryo extract containing various concentrations of corticosterone. Normal pancreatic exocrine morphology was preserved. In addition, media amylase concentration remained high and media insulin was suppressed. This is in sharp contrast to explants incubated on control medium without addition of steroid in which a rapid dissappearance of the acinar component and a selective proliferation of the islet cells was noted. The magnitude of these effects was related to the concentration of the steroid. Corticosterone was effective in preserving pancreatic acinar cells through 8-10 days in vitro. Removal of high levels of corticosterone from the incubation medium after 4 days of culture resulted in a decrease in media amylase and a fall in explant acinar cell mass. The media insulin returned to control levels during the following 4 days of culture. Addition of corticosterone to the media following 4 days of control culture resulted in no increase in media amylase and no statistically significant differences in explant acinar cell mass. Media insulin was decreased from control levels following the additional 4 days of incubation. However, corticosterone, even at a concentration of 10.0 mug/ml, was not effective in depressing insulin secretion as was foetal adrenal co-culture. It is proposed that adrenal corticosteroids are responsible for the maintenance of differentiated acinar cells previously observed in pancreatic adrenal co-culture. This suggests that corticosteroids may play an important role in in vivo pancreatic morphogenesis and cytodifferentiation. In addition, adrenal corticosteroids directly inhibit insulin release from the explant beta cells in vitro.", "PMID": 976649}
{"id": "pubmed23n0032_10793", "title": "Transcription of repetitive and unique DNA nucleotide sequences in pigeon erythroid cells with different degrees of specialization.", "content": "HnRNA fractions with sedimentation coefficients greater than 45 S isolated from pigeon bone marrow as well as from the immature and mature erythroid cells of periferal blood were hybridised with a large excess of DNA fractionated on the basis of renaturation kinetics. 58-62% of the input RNAs were recovered as RNAase-resistant hybrids. About 1/3 (20%) of bone marrow greater than 45 S RNA found in the hybrids was hybridised with the repetitive and baout 2/3 (40%) with the unique DNA sequences. In addition, a considerably smaller portion of greater than 45 S RNA from the \"reticulocytes\" (13%) and \"erythrocytes\" (approximately 6%) was hybridised with the repetitive DNA.", "contents": "Transcription of repetitive and unique DNA nucleotide sequences in pigeon erythroid cells with different degrees of specialization. HnRNA fractions with sedimentation coefficients greater than 45 S isolated from pigeon bone marrow as well as from the immature and mature erythroid cells of periferal blood were hybridised with a large excess of DNA fractionated on the basis of renaturation kinetics. 58-62% of the input RNAs were recovered as RNAase-resistant hybrids. About 1/3 (20%) of bone marrow greater than 45 S RNA found in the hybrids was hybridised with the repetitive and baout 2/3 (40%) with the unique DNA sequences. In addition, a considerably smaller portion of greater than 45 S RNA from the \"reticulocytes\" (13%) and \"erythrocytes\" (approximately 6%) was hybridised with the repetitive DNA.", "PMID": 976650}
{"id": "pubmed23n0032_10794", "title": "An electrophoretic analysis of RNA synthesis in normal and lobeless Ilyanassa embryo.", "content": "RNA from pulse-labelled normal and lobeless Illyanassa embryos was analysed by electrophoresis on polyacrylamide gels. The proportional distribution of radioactivity among the RNAs made by these two types of embryos was compared statistically. It was shown that removal of the third polar lobe from the Ilyanassa egg at first cleavage did not produce any change in the proportion of several size classes of RNA transcribed during the first day od development. The post-gastrular lobeless embryo did show a significant deviation from the normal pattern of RNA synthesis, though it is not clear that this was a direct effect on transcription by the polar lobe cytoplasm.", "contents": "An electrophoretic analysis of RNA synthesis in normal and lobeless Ilyanassa embryo. RNA from pulse-labelled normal and lobeless Illyanassa embryos was analysed by electrophoresis on polyacrylamide gels. The proportional distribution of radioactivity among the RNAs made by these two types of embryos was compared statistically. It was shown that removal of the third polar lobe from the Ilyanassa egg at first cleavage did not produce any change in the proportion of several size classes of RNA transcribed during the first day od development. The post-gastrular lobeless embryo did show a significant deviation from the normal pattern of RNA synthesis, though it is not clear that this was a direct effect on transcription by the polar lobe cytoplasm.", "PMID": 976651}
{"id": "pubmed23n0032_10795", "title": "Isoenzymes of acid phosphatase and non-specific esterases in cultures of neoplastic and normal tobacco tissues.", "content": "Axenic cultures of normal, habituated and crown gall teratoma were grown under varying conditions to examine the effects of environment on the expression of neoplastic character. Acid phosphatase patterns on polyacrylamide gels did not vary greatly among tissues although there were differences in acid phosphatase activity between various strains of Agrobacterium tumefaciens, the bacteria which cause crown gall. Certain esterase isoenzymes were found only in tissues grown on specific media, while others were tissue-specific but independent of the nature of the medium. Comparisons of liquid and solid grown cultures revealed that culture conditions also influence esterase expression. Both sunflower and tobacco crown gall tissue contained an esterase not found in habituated or normal tissues, and similar in electrophoretic mobility to an esterase found in extracts of the bacteria that had induced the tumors. The basic difference between the three tissue types studied is the manner in which they respond to a given environment.", "contents": "Isoenzymes of acid phosphatase and non-specific esterases in cultures of neoplastic and normal tobacco tissues. Axenic cultures of normal, habituated and crown gall teratoma were grown under varying conditions to examine the effects of environment on the expression of neoplastic character. Acid phosphatase patterns on polyacrylamide gels did not vary greatly among tissues although there were differences in acid phosphatase activity between various strains of Agrobacterium tumefaciens, the bacteria which cause crown gall. Certain esterase isoenzymes were found only in tissues grown on specific media, while others were tissue-specific but independent of the nature of the medium. Comparisons of liquid and solid grown cultures revealed that culture conditions also influence esterase expression. Both sunflower and tobacco crown gall tissue contained an esterase not found in habituated or normal tissues, and similar in electrophoretic mobility to an esterase found in extracts of the bacteria that had induced the tumors. The basic difference between the three tissue types studied is the manner in which they respond to a given environment.", "PMID": 976652}
{"id": "pubmed23n0032_10796", "title": "[Use of atrial pacing in diagnosis of coronary insufficiency. Comparison between coronarography and exercise test (author's transl)].", "content": "--123 atrial pacings (AP) performed as diagnostic investigations in patients with chest pains were re-examined. By using floating catheter without fluoroscopic control, this technique is very simple to perform and free from relevant risks. The diagnostic sensibility and specificity of AP were examined in 93 patients in which a coronary arteriography was performed; these figures were compared with the corresponding values observed in 65 patients in which an adequate diagnostic exercise test (ET) was also available. The diagnostic sensibility of AP examined in 63 patients with significant coronary artery disease was 90%; the corresponding value of ET was 79%. In particular, in patients with single vessel disease, the sensibility of AP (90%) was much higher than that observed in ET (40%). The specificity of AP examined in 30 patients free from significant stenosis of the coronary arterial tree was 43%. This value was largely lower than that observed in ET (82%) in the same patients, and appears to be inadequate for validation AP as a diagnostic tool in coronary heart disease. Therefore, AP must be limited to functional, and not diagnostic, evaluation of patients in which the diagnosis of coronary heart disease can be made by other means.", "contents": "[Use of atrial pacing in diagnosis of coronary insufficiency. Comparison between coronarography and exercise test (author's transl)]. --123 atrial pacings (AP) performed as diagnostic investigations in patients with chest pains were re-examined. By using floating catheter without fluoroscopic control, this technique is very simple to perform and free from relevant risks. The diagnostic sensibility and specificity of AP were examined in 93 patients in which a coronary arteriography was performed; these figures were compared with the corresponding values observed in 65 patients in which an adequate diagnostic exercise test (ET) was also available. The diagnostic sensibility of AP examined in 63 patients with significant coronary artery disease was 90%; the corresponding value of ET was 79%. In particular, in patients with single vessel disease, the sensibility of AP (90%) was much higher than that observed in ET (40%). The specificity of AP examined in 30 patients free from significant stenosis of the coronary arterial tree was 43%. This value was largely lower than that observed in ET (82%) in the same patients, and appears to be inadequate for validation AP as a diagnostic tool in coronary heart disease. Therefore, AP must be limited to functional, and not diagnostic, evaluation of patients in which the diagnosis of coronary heart disease can be made by other means.", "PMID": 976655}
{"id": "pubmed23n0032_10797", "title": "[Coronarographic and ventriculographic aspects of 76 cases of unstable angina (authors transl)].", "content": "-- Coronarographic and ventriculographic aspects of 76 patients with unstable angina were analyzed. 8 cases (10%) had normal coronary arteries, 9 (11.8%) had slight stenotic lesions (less than 50%), 59 (77.6%) had serious stenosis. The stenosis concerned only one principal branch in 22 patients (37.2%), 2 branches in 24 (40%), 3 branches in 13 (22%). In 77-79% of cases, ventricular contractility was normal. A collateral circulation was found in 58% of cases, but no link was discovered between the coronaric lesions and the clinical type of angina. The course of the illness was studied during the hospitalization and post-hospitalization period for an average observation time of 14 months (55 cases). The course of illness type depends on the number of affected branches and the myocardial contractility. In 25% of the patients with lesions in 3 branches there was an unfavourable course of illness (either infarction or death), whilst where there were lesions in one or two branches, negative results were 11% and 15% respectively. Patients with normal coronary arteries or slight stenosis had no unfavourable course of illness. In 37% of patients with altered contractility, an unfavourable course of illness was found, as against 27% with normal contractility. The presence of collateral circulation doesn't seem to influence the course the illness takes. A coronarographic examination seems to be an essential elelment in deciding on the prognosis of unstable angina.", "contents": "[Coronarographic and ventriculographic aspects of 76 cases of unstable angina (authors transl)]. -- Coronarographic and ventriculographic aspects of 76 patients with unstable angina were analyzed. 8 cases (10%) had normal coronary arteries, 9 (11.8%) had slight stenotic lesions (less than 50%), 59 (77.6%) had serious stenosis. The stenosis concerned only one principal branch in 22 patients (37.2%), 2 branches in 24 (40%), 3 branches in 13 (22%). In 77-79% of cases, ventricular contractility was normal. A collateral circulation was found in 58% of cases, but no link was discovered between the coronaric lesions and the clinical type of angina. The course of the illness was studied during the hospitalization and post-hospitalization period for an average observation time of 14 months (55 cases). The course of illness type depends on the number of affected branches and the myocardial contractility. In 25% of the patients with lesions in 3 branches there was an unfavourable course of illness (either infarction or death), whilst where there were lesions in one or two branches, negative results were 11% and 15% respectively. Patients with normal coronary arteries or slight stenosis had no unfavourable course of illness. In 37% of patients with altered contractility, an unfavourable course of illness was found, as against 27% with normal contractility. The presence of collateral circulation doesn't seem to influence the course the illness takes. A coronarographic examination seems to be an essential elelment in deciding on the prognosis of unstable angina.", "PMID": 976656}
{"id": "pubmed23n0032_10798", "title": "[Variable aspects in mitral valve prolapse. Echographic and phonocardiographic studies of 68 cases (author's transl)].", "content": "Previous parallel echocardiographic-angiocardiographic studies have proven specificity and sensitivity of ultrasonography in diagnosing prolapse of the mitral valve. Echocardiograms and phonocardiograms from 68 patients with mitral valve prolapse were studied. Echocardiograms suggestive of mitral valve prolapse were obtained in 66 patients. The typical abnormality consisted of an abrupt posterior displacement of the mitral valve leaflets, either late systolic or pansystolic. Moreover a new pattern not previously described was observed, i.e. an abrupt isolated early systolic posterior motion of the mitral leaflets (\"early prolapse\"). Phonocardiography, on the other hand, showed a variable spectrum of acoustic findings: non-ejection systolic click and/or late systolic murmur, pansytolic murmur, early systolic click,ejectioarly systolic click, ejection murmur, in variable association, and finally no abnormal sound at all (\"silent prolapse\"). The authors conclude that mitral valve prolapse may be suspected, whatever the auscultatory finding. Echocardiography provides a useful, non invasive method for detecting those forms which also present an uncommon auscultatory pattern.", "contents": "[Variable aspects in mitral valve prolapse. Echographic and phonocardiographic studies of 68 cases (author's transl)]. Previous parallel echocardiographic-angiocardiographic studies have proven specificity and sensitivity of ultrasonography in diagnosing prolapse of the mitral valve. Echocardiograms and phonocardiograms from 68 patients with mitral valve prolapse were studied. Echocardiograms suggestive of mitral valve prolapse were obtained in 66 patients. The typical abnormality consisted of an abrupt posterior displacement of the mitral valve leaflets, either late systolic or pansystolic. Moreover a new pattern not previously described was observed, i.e. an abrupt isolated early systolic posterior motion of the mitral leaflets (\"early prolapse\"). Phonocardiography, on the other hand, showed a variable spectrum of acoustic findings: non-ejection systolic click and/or late systolic murmur, pansytolic murmur, early systolic click,ejectioarly systolic click, ejection murmur, in variable association, and finally no abnormal sound at all (\"silent prolapse\"). The authors conclude that mitral valve prolapse may be suspected, whatever the auscultatory finding. Echocardiography provides a useful, non invasive method for detecting those forms which also present an uncommon auscultatory pattern.", "PMID": 976657}
{"id": "pubmed23n0032_10799", "title": "[Phonomechanocardiographic diagnosis of the apparently \"silent\" mitral valve prolapse syndrome (author's transl)].", "content": "Five cases with angiocardiographically diagnosed posterior mitral valve prolapse and without ausculatotory findings of midsystolic click or late systolic murmur were studied with phonocardiographic technique employing pharmacological tests and postural changes. A midsystolic click was thus obtained. Isoprenaline was the must helpful drug to achieve it. The accompanying electrocardiographic and coronarographic features of this syndrome are described and discussed.", "contents": "[Phonomechanocardiographic diagnosis of the apparently \"silent\" mitral valve prolapse syndrome (author's transl)]. Five cases with angiocardiographically diagnosed posterior mitral valve prolapse and without ausculatotory findings of midsystolic click or late systolic murmur were studied with phonocardiographic technique employing pharmacological tests and postural changes. A midsystolic click was thus obtained. Isoprenaline was the must helpful drug to achieve it. The accompanying electrocardiographic and coronarographic features of this syndrome are described and discussed.", "PMID": 976658}
{"id": "pubmed23n0032_10800", "title": "Influence of \"Polarizing\" infusions on anthiarrhythmic effects of beta-blockers.", "content": "The antiarrhythmic effect of practolol (0.30 mg/kg) was studied in 25 patients pretreated with infusion of glucose-insulin (GI) solution. GI solution showed an antiarrhythmic effect either after or before the beta-blocker with pH in normal range or compensated metabolic acidosis. GI infusion produced a metabolic acidosis and an arrhythmogenic effect in some cases. Practolol had no antiarrhythmic effect in patients with VPB and GI infusion raised metabolic acidosis.", "contents": "Influence of \"Polarizing\" infusions on anthiarrhythmic effects of beta-blockers. The antiarrhythmic effect of practolol (0.30 mg/kg) was studied in 25 patients pretreated with infusion of glucose-insulin (GI) solution. GI solution showed an antiarrhythmic effect either after or before the beta-blocker with pH in normal range or compensated metabolic acidosis. GI infusion produced a metabolic acidosis and an arrhythmogenic effect in some cases. Practolol had no antiarrhythmic effect in patients with VPB and GI infusion raised metabolic acidosis.", "PMID": 976659}
{"id": "pubmed23n0032_10801", "title": "[Early changes of respiratory function in mitral valve stenosis].", "content": "In order to detect early changes of respiratory function in patients affects by pure mitral valve stenosis, the authors selected 12 patients-non smokers, without symptoms of respiratory disease, of I and II NYHA class. In all subjects right and left cardiac catheterization and conventional spirometric measurements were performed. Then maximal mid-expiratory flow (MMEF), maximal expiratory flow at 50% of vital capacity (MEF 50%), maximal expiratory flow at 25% of vital capacity (MEF 25%), closing volume (CV) and closing capacity (CC) were determined, to find a small airways (bronchi of caliber inferior to 2 mm) disease. Whereas conventional spirometric measurements showed normal values, the small airways disease was proved by MEF 50% and MEF 25% measurements. The small airways obstruction observed by the authors may be due to: a) dilatation of pulmonary vessels because of venous congestion resulting in the compression of adjacent small airways; b) partial bronchiolar obstruction because of congestion of submucous venous plexus; c) interstitial oedema due to increase of extravascular pulmonary water because of pulmonary venous congestion.", "contents": "[Early changes of respiratory function in mitral valve stenosis]. In order to detect early changes of respiratory function in patients affects by pure mitral valve stenosis, the authors selected 12 patients-non smokers, without symptoms of respiratory disease, of I and II NYHA class. In all subjects right and left cardiac catheterization and conventional spirometric measurements were performed. Then maximal mid-expiratory flow (MMEF), maximal expiratory flow at 50% of vital capacity (MEF 50%), maximal expiratory flow at 25% of vital capacity (MEF 25%), closing volume (CV) and closing capacity (CC) were determined, to find a small airways (bronchi of caliber inferior to 2 mm) disease. Whereas conventional spirometric measurements showed normal values, the small airways disease was proved by MEF 50% and MEF 25% measurements. The small airways obstruction observed by the authors may be due to: a) dilatation of pulmonary vessels because of venous congestion resulting in the compression of adjacent small airways; b) partial bronchiolar obstruction because of congestion of submucous venous plexus; c) interstitial oedema due to increase of extravascular pulmonary water because of pulmonary venous congestion.", "PMID": 976660}
{"id": "pubmed23n0032_10802", "title": "[\"Peripheral infarction blocks\". Vectocardiographic analysis and electrogenetic hypothesis (author's transl)].", "content": "500 vectorcardiograms of variously located myocardial infarctions were analysed. Abnormalities of the afferent limb of QRS loop, consistent with intraventricular conduction disturbances, were detected in 42 cases. This pattern, according to Rosenbaum, was called \"infarction block\"; it was always associated with inferior and/or posterior infarction. This kind of block is shown by a large downward, righward and backward-directed vector, which always appears about 50 msec after the onset of ventricular activation. Total ventricular depolarization time was not significantly prolonged. The pattern was explained as a variant of \"peri-infarction block\" and its origin is hypothesized as the interruption of a peripheral ramification of LBB posterior fascicle.", "contents": "[\"Peripheral infarction blocks\". Vectocardiographic analysis and electrogenetic hypothesis (author's transl)]. 500 vectorcardiograms of variously located myocardial infarctions were analysed. Abnormalities of the afferent limb of QRS loop, consistent with intraventricular conduction disturbances, were detected in 42 cases. This pattern, according to Rosenbaum, was called \"infarction block\"; it was always associated with inferior and/or posterior infarction. This kind of block is shown by a large downward, righward and backward-directed vector, which always appears about 50 msec after the onset of ventricular activation. Total ventricular depolarization time was not significantly prolonged. The pattern was explained as a variant of \"peri-infarction block\" and its origin is hypothesized as the interruption of a peripheral ramification of LBB posterior fascicle.", "PMID": 976661}
{"id": "pubmed23n0032_10803", "title": "[Echocardiographic diagnosis of the dissection of the thoracic aorta].", "content": "Nine patients with aortic root dilatation without dissection and four patients with a dissecting aneurysm of the ascending aorta were studied with single-plane echocardiography. Previously reported echocardiographic findings, which have been shown to be similar in these two groups of patients, are confirmed. In addition to (a) aortic root dilatation, (b) thickening of the anterior and/or the posterior aortic wall, and (c) the multiple parallel echoes within the widened aortic walls, which are all considered to be not specific signs of dissection, the authors outline the presence of some specific echocardiographic patterns in patients with a dissecting hematoma of the ascending aorta. These include: 1) the loss of contiguity between the inner borders of the anterior aortic wall and the interventricular septum when the dissection is confined to the anterior wall of the aorta; 2) the loss of contiguity between the inner and/or outer borders of the posterior aortic wall and the mitral anulus if the hematoma extends to the posterior wall of the aorta.", "contents": "[Echocardiographic diagnosis of the dissection of the thoracic aorta]. Nine patients with aortic root dilatation without dissection and four patients with a dissecting aneurysm of the ascending aorta were studied with single-plane echocardiography. Previously reported echocardiographic findings, which have been shown to be similar in these two groups of patients, are confirmed. In addition to (a) aortic root dilatation, (b) thickening of the anterior and/or the posterior aortic wall, and (c) the multiple parallel echoes within the widened aortic walls, which are all considered to be not specific signs of dissection, the authors outline the presence of some specific echocardiographic patterns in patients with a dissecting hematoma of the ascending aorta. These include: 1) the loss of contiguity between the inner borders of the anterior aortic wall and the interventricular septum when the dissection is confined to the anterior wall of the aorta; 2) the loss of contiguity between the inner and/or outer borders of the posterior aortic wall and the mitral anulus if the hematoma extends to the posterior wall of the aorta.", "PMID": 976662}
{"id": "pubmed23n0032_10804", "title": "[Study of the descending phase of the aortic pressure curve. Possible applications to the monitoring of peripheral resistance and arterial compliance (author's transl)].", "content": "In 21 patients aortic pressure was recorded just above the diaphragm. The curve after the dicrotic notch is an exponential function of the time, and it is possible to characterize it by a unique time-constant. This constnat is shown to depend on aortic compliance and peripheral resistance: as aortic compliance does not vary abruptly in physiological conditions, this allows monitoring of peripheral resistance by monitoring time constant. Mathematical bases of time constant and of the peripheral resistance are briefly discussed.", "contents": "[Study of the descending phase of the aortic pressure curve. Possible applications to the monitoring of peripheral resistance and arterial compliance (author's transl)]. In 21 patients aortic pressure was recorded just above the diaphragm. The curve after the dicrotic notch is an exponential function of the time, and it is possible to characterize it by a unique time-constant. This constnat is shown to depend on aortic compliance and peripheral resistance: as aortic compliance does not vary abruptly in physiological conditions, this allows monitoring of peripheral resistance by monitoring time constant. Mathematical bases of time constant and of the peripheral resistance are briefly discussed.", "PMID": 976663}
{"id": "pubmed23n0032_10805", "title": "Experimental revascularization of acute myocardial infarction. III: Correlation between the behaviour of the free nucleotide acid solubles and some aliphatic biogenic amines.", "content": "Free nucleotide, polyamine and nucleic acid synthesis was studied in myocardial acute ischemia and reperfusion. An early stimulation of biosynthesis of these compounds was observed during ischemia, than later, a remarkable decrease of their specific radioactivity appeared. Reperfusion experiments cause a significant increase of nucleic acids and related compound biosynthesis. The results indicate that the biochemical mechanism of myocardial protein synthesis, under our experimental conditions are not irreversibly damaged by ischemia. In fact, the  reperfusion causes a remarkable recovery of nucleic acid, free nucleotide and polyamine specific radioactivity. The changes during the period of infarction and subsequent reperfusion show that polyamines play a key role in myocardial ischemia and reperfusion.", "contents": "Experimental revascularization of acute myocardial infarction. III: Correlation between the behaviour of the free nucleotide acid solubles and some aliphatic biogenic amines. Free nucleotide, polyamine and nucleic acid synthesis was studied in myocardial acute ischemia and reperfusion. An early stimulation of biosynthesis of these compounds was observed during ischemia, than later, a remarkable decrease of their specific radioactivity appeared. Reperfusion experiments cause a significant increase of nucleic acids and related compound biosynthesis. The results indicate that the biochemical mechanism of myocardial protein synthesis, under our experimental conditions are not irreversibly damaged by ischemia. In fact, the  reperfusion causes a remarkable recovery of nucleic acid, free nucleotide and polyamine specific radioactivity. The changes during the period of infarction and subsequent reperfusion show that polyamines play a key role in myocardial ischemia and reperfusion.", "PMID": 976665}
{"id": "pubmed23n0032_10806", "title": "[Anomalous origin of the left coronary artery from the pulmonary artery (author's transl)].", "content": "The case of a three year-old child with anomalous origin of the left coronary artery from the pulmonary artery is presented. He had a clinical picture and an electrocardiographic pattern of antero-lateral myocardial infarction that suggested a cardiac malformation. Once the diagnosis was established by cardiac catheterization and coronary arteriography, the defect was successfully correted by transplanting the anomalous coronary vessel to the aorta. The clinical, electrocardiographic and angiographic features of the anomaly are given.", "contents": "[Anomalous origin of the left coronary artery from the pulmonary artery (author's transl)]. The case of a three year-old child with anomalous origin of the left coronary artery from the pulmonary artery is presented. He had a clinical picture and an electrocardiographic pattern of antero-lateral myocardial infarction that suggested a cardiac malformation. Once the diagnosis was established by cardiac catheterization and coronary arteriography, the defect was successfully correted by transplanting the anomalous coronary vessel to the aorta. The clinical, electrocardiographic and angiographic features of the anomaly are given.", "PMID": 976667}
{"id": "pubmed23n0032_10807", "title": "[Rupture of interventricular septum secondary to blunt chest trauma. Report of a case surgically treated with success (author's transl)].", "content": "A case of interventricular septal defect secondary to blunt chest trauma is reported. It was characterized by a disconnection of the interventricular muscolar septal in the  anterosuperior part of the heart wall. The diagnosis, suspected by clinical and instrumental parameters, was definitely confirmedy by hemodynamic and contrastographic examination. Repair surgery of the defect with a dacron patch was performed, using extracorporea normothermic circulation seven months after the accident. The patient was examined three months and twelve months after the operation; the clinical examinations did not reveal any cardiac murmur and the patient's health was satisfactory. The incidence, mechanism of rupture of interventricular septum and the main surgical and clinical aspects of this type of pathology are discussed.", "contents": "[Rupture of interventricular septum secondary to blunt chest trauma. Report of a case surgically treated with success (author's transl)]. A case of interventricular septal defect secondary to blunt chest trauma is reported. It was characterized by a disconnection of the interventricular muscolar septal in the  anterosuperior part of the heart wall. The diagnosis, suspected by clinical and instrumental parameters, was definitely confirmedy by hemodynamic and contrastographic examination. Repair surgery of the defect with a dacron patch was performed, using extracorporea normothermic circulation seven months after the accident. The patient was examined three months and twelve months after the operation; the clinical examinations did not reveal any cardiac murmur and the patient's health was satisfactory. The incidence, mechanism of rupture of interventricular septum and the main surgical and clinical aspects of this type of pathology are discussed.", "PMID": 976668}
{"id": "pubmed23n0032_10808", "title": "Peptic ulcer in diabetes mellitus.", "content": "Twelve cases of peptic ulcer with diabetes mellitus were found in 165 hospitalized diabetics. All of them had gastric ulcer and no duodenal ulcers were found. The incidence of peptic ulcer in diabetics was comparatively higher than the previously reported series. But there was nosignificant correlation between the duration of diabetes and the onset of gastric ulcer. The gastric ulcer with poorly controlled diabetes showed more intractability than those without triopathy and well-controlled diabetes.", "contents": "Peptic ulcer in diabetes mellitus. Twelve cases of peptic ulcer with diabetes mellitus were found in 165 hospitalized diabetics. All of them had gastric ulcer and no duodenal ulcers were found. The incidence of peptic ulcer in diabetics was comparatively higher than the previously reported series. But there was nosignificant correlation between the duration of diabetes and the onset of gastric ulcer. The gastric ulcer with poorly controlled diabetes showed more intractability than those without triopathy and well-controlled diabetes.", "PMID": 976682}
{"id": "pubmed23n0032_10809", "title": "Ultrastructural studies of experimental acute pancreatitis.", "content": "Acute pancreatitis was studied by electron microscopy after retrograde infusion of either trypsin, and/or beta-glucuronidase into the canine pancreatic duct. Marked changes were induced by the mixture of trypsin and beta-glucuronidase. (1) The acinar cells were initially excavated from the acinar lumen and formed cystic bodies in themselves. The cystic bodies were then disrupted at their marginal membranes, and the acinar cells were filled with a large amount of fibrillar materials which originated from the contents of the cystic bodies. At this time, the luminal margin of the acinar cells completely disappeared. (2) The cellular organellas and the intracellular fibrillar materials in the acinar cells were discharged into the interstitial space through the disrupted basal lamina. Infection in the pancreatic ductal system was considered to play an important role in the pathogenesis of acute hemorrhagic pancreatitis.", "contents": "Ultrastructural studies of experimental acute pancreatitis. Acute pancreatitis was studied by electron microscopy after retrograde infusion of either trypsin, and/or beta-glucuronidase into the canine pancreatic duct. Marked changes were induced by the mixture of trypsin and beta-glucuronidase. (1) The acinar cells were initially excavated from the acinar lumen and formed cystic bodies in themselves. The cystic bodies were then disrupted at their marginal membranes, and the acinar cells were filled with a large amount of fibrillar materials which originated from the contents of the cystic bodies. At this time, the luminal margin of the acinar cells completely disappeared. (2) The cellular organellas and the intracellular fibrillar materials in the acinar cells were discharged into the interstitial space through the disrupted basal lamina. Infection in the pancreatic ductal system was considered to play an important role in the pathogenesis of acute hemorrhagic pancreatitis.", "PMID": 976683}
{"id": "pubmed23n0032_10810", "title": "Clinical investigation on abnormal glucose tolerance in liver cirrhosis.", "content": "The changes of blood glucose, serum insulin, serum free fatty acid and its fatty acid composition following oral glucose load were observed in twenty-nine cirrhotic patients. The insulin secretory response was significantly lower in the cirrhotic patients with overt diabetes than in those without overt diabetes. There were no definite relation between serum free fatty acid level or its composition and glucose intolerance. These results suggest that the diabetic state in most of the cirrhotic patients with overt diabetes is due to essential diabetes and that serum free fatty acid livel and its composition are not important factors contributory to the glucose intolerance in liver cirrhosis.", "contents": "Clinical investigation on abnormal glucose tolerance in liver cirrhosis. The changes of blood glucose, serum insulin, serum free fatty acid and its fatty acid composition following oral glucose load were observed in twenty-nine cirrhotic patients. The insulin secretory response was significantly lower in the cirrhotic patients with overt diabetes than in those without overt diabetes. There were no definite relation between serum free fatty acid level or its composition and glucose intolerance. These results suggest that the diabetic state in most of the cirrhotic patients with overt diabetes is due to essential diabetes and that serum free fatty acid livel and its composition are not important factors contributory to the glucose intolerance in liver cirrhosis.", "PMID": 976684}
{"id": "pubmed23n0032_10811", "title": "Serum gastrin and gastrin-like immunoreactivity of gastrointestinal mucosa in fetal, neonatal and adult rats.", "content": "Serum gastrin concentration became became detectable in rats at one week after birth (56 pg/ml), and a rapid rise to 198 pg/ml was seen at two weeks after birth. Gradual decreases were seen again at three weeks and four weeks, and thereafter it attained the adult level of 132 +/- 17.6 pg/ml. In regards to the gastrin-like immunoreactivity (GLI) in the gastrointestinal mucosa during the fetal period, the values were extremely low even in the antral mucosa. However, the GLI showed an increase during the neonatal suckling period, and was accompanied by a remarkable increase at the commencement of feeding. This GLI coincided with so-called little gastrin, and while an increase accompanied by the development of growth was seen, no qualitative changes were evident. GLI values were high in the duodenal mucosa followed by the antrum, and positive evidence was also seen in the corpus of the stomach and jejunum. High values of GLI in the mucosae were gradually seen at two weeks after birth coinciding with the rise in serum gastrin levels, and the significance of the increases was discussed from the anlge of the trophic effect of gastrin.", "contents": "Serum gastrin and gastrin-like immunoreactivity of gastrointestinal mucosa in fetal, neonatal and adult rats. Serum gastrin concentration became became detectable in rats at one week after birth (56 pg/ml), and a rapid rise to 198 pg/ml was seen at two weeks after birth. Gradual decreases were seen again at three weeks and four weeks, and thereafter it attained the adult level of 132 +/- 17.6 pg/ml. In regards to the gastrin-like immunoreactivity (GLI) in the gastrointestinal mucosa during the fetal period, the values were extremely low even in the antral mucosa. However, the GLI showed an increase during the neonatal suckling period, and was accompanied by a remarkable increase at the commencement of feeding. This GLI coincided with so-called little gastrin, and while an increase accompanied by the development of growth was seen, no qualitative changes were evident. GLI values were high in the duodenal mucosa followed by the antrum, and positive evidence was also seen in the corpus of the stomach and jejunum. High values of GLI in the mucosae were gradually seen at two weeks after birth coinciding with the rise in serum gastrin levels, and the significance of the increases was discussed from the anlge of the trophic effect of gastrin.", "PMID": 976685}
{"id": "pubmed23n0032_10812", "title": "Vitamin B2 metabolism in the isolated perfused rat liver.", "content": "An investigation on vitamin B2 (V.B2) metabolism was undertaken, using perfused livers isolated from 1. normal rats, 2. riboflavin deficient rats, 3. choline deficient rats with fatty cirrhtic livers, and 4. alloxan diabetic rats, without the effect of extrahepatic factors. From the results, it was concluded that phosphorylation of V.B2 was performed in the liver, and was remarkably reduced in fatty cirrhosis. Moreover, in alloxan diabetic liver, the affected phosphorylation of V.B2 was improved by the infusion of insulin. The important role that liver performs on V.B2 metabolism, can be emphasized from these findings.", "contents": "Vitamin B2 metabolism in the isolated perfused rat liver. An investigation on vitamin B2 (V.B2) metabolism was undertaken, using perfused livers isolated from 1. normal rats, 2. riboflavin deficient rats, 3. choline deficient rats with fatty cirrhtic livers, and 4. alloxan diabetic rats, without the effect of extrahepatic factors. From the results, it was concluded that phosphorylation of V.B2 was performed in the liver, and was remarkably reduced in fatty cirrhosis. Moreover, in alloxan diabetic liver, the affected phosphorylation of V.B2 was improved by the infusion of insulin. The important role that liver performs on V.B2 metabolism, can be emphasized from these findings.", "PMID": 976687}
{"id": "pubmed23n0032_10813", "title": "Immunological studies on drug-induced allergic hepatitis.", "content": "In many cases of drug-induced allergic hepatitis, peripheral lymphocytes were transformed by the stimulation with a given drug in the presence of autologous serum. However, when rat liver microsome fraction of soluble liver specific antigen fraction was added to the culture instead of autologous serum, the drug-induced lymphocyte transformation was more efficiently seen than autologus serum, while rat liver mitochondria fraction was less effective. On the other hand, in the cases of allergic drug eruption which did not show any liver injury, the addition of liver subcellular fractions were much less effective to induce the lymphocyte transformation than autologous serum. These results may suggest that liver subcellular component is involved in pathogenesis of drug-induced allergic hepatitis.", "contents": "Immunological studies on drug-induced allergic hepatitis. In many cases of drug-induced allergic hepatitis, peripheral lymphocytes were transformed by the stimulation with a given drug in the presence of autologous serum. However, when rat liver microsome fraction of soluble liver specific antigen fraction was added to the culture instead of autologous serum, the drug-induced lymphocyte transformation was more efficiently seen than autologus serum, while rat liver mitochondria fraction was less effective. On the other hand, in the cases of allergic drug eruption which did not show any liver injury, the addition of liver subcellular fractions were much less effective to induce the lymphocyte transformation than autologous serum. These results may suggest that liver subcellular component is involved in pathogenesis of drug-induced allergic hepatitis.", "PMID": 976688}
{"id": "pubmed23n0032_10814", "title": "Effect of premedication of coenzyme Q10 on pancreozymin-induced pancreatic secretion in rats.", "content": "Effect of Coenzyme Q10 (CoQ10), an essential coenzyme in oxidative phosphorylation in the mitochondria, was studied in the rat's pancreas. A dose of pancreozymin (0.2 u/100 g body wt) was injected into the femoral vein at 20 min intervals. The initial injection produced a definite increase in amylase output and the pancreatic juice flow. Subsequent injections, however, caused smaller responses than the preceding (tachyphylaxis). When CoQ10 (3 mg/100 g body wt) had been administrated 1 hour prior to the initial stimulation, both the amylase output and the rate of juice flow were significantly enhanced: the tachyphylaxis almost disappeared. Effect of CoQ10 was also observed in the isolated and perfused pancreas. CoQ10 produced slight increase in pancreozymin-induced amylase output, but the increase was not significant. The effect of CoQ10, to increase the pancreatic blood flow, seems to be involved in the in situ effect of CoQ10 in cooperation with the direct effect on pancreatic acinar cells.", "contents": "Effect of premedication of coenzyme Q10 on pancreozymin-induced pancreatic secretion in rats. Effect of Coenzyme Q10 (CoQ10), an essential coenzyme in oxidative phosphorylation in the mitochondria, was studied in the rat's pancreas. A dose of pancreozymin (0.2 u/100 g body wt) was injected into the femoral vein at 20 min intervals. The initial injection produced a definite increase in amylase output and the pancreatic juice flow. Subsequent injections, however, caused smaller responses than the preceding (tachyphylaxis). When CoQ10 (3 mg/100 g body wt) had been administrated 1 hour prior to the initial stimulation, both the amylase output and the rate of juice flow were significantly enhanced: the tachyphylaxis almost disappeared. Effect of CoQ10 was also observed in the isolated and perfused pancreas. CoQ10 produced slight increase in pancreozymin-induced amylase output, but the increase was not significant. The effect of CoQ10, to increase the pancreatic blood flow, seems to be involved in the in situ effect of CoQ10 in cooperation with the direct effect on pancreatic acinar cells.", "PMID": 976689}
{"id": "pubmed23n0032_10815", "title": "Clinical effects of anticancer drugs to pancreatic diseases as protein synthesis inhibitors.", "content": "The anticancer drugs, like 5-Fluorouracil, which are believed to interfere with enzyme protein synthesis in the exocrine cells of pancreas were administered intravenously to fifteen patients with various pancreatic diseases. The improvement of clinical symptoms and the diminution of serum and urinary amylase levels were observed in four cases with acute pancreatitis and two cases with chronic relapsing pancreatitis. The postoperative complications, namely the formation of pancreatic fistula and the rupture of pancreaticojejunostomy, or the aggravation of concomitant pancreatitis were not observed in three cases with benign surgical pancreatic diseases and six cases with pancreatic carcinoma. Furthermore, the diminution of amylase and protein output of pancreatic juice from canulae inserted into pancreatic ducts were observed.", "contents": "Clinical effects of anticancer drugs to pancreatic diseases as protein synthesis inhibitors. The anticancer drugs, like 5-Fluorouracil, which are believed to interfere with enzyme protein synthesis in the exocrine cells of pancreas were administered intravenously to fifteen patients with various pancreatic diseases. The improvement of clinical symptoms and the diminution of serum and urinary amylase levels were observed in four cases with acute pancreatitis and two cases with chronic relapsing pancreatitis. The postoperative complications, namely the formation of pancreatic fistula and the rupture of pancreaticojejunostomy, or the aggravation of concomitant pancreatitis were not observed in three cases with benign surgical pancreatic diseases and six cases with pancreatic carcinoma. Furthermore, the diminution of amylase and protein output of pancreatic juice from canulae inserted into pancreatic ducts were observed.", "PMID": 976690}
{"id": "pubmed23n0032_10816", "title": "Comparison of pancreatic dysfunction in chronic alcoholic pancreatitis and chronic gallstone pancreatitis.", "content": "Comparative assessments of the endocrine and exocrine functions of the pancreas associated with gallstone pancreatitis or alcoholic pancreatitis were performed in a series of 86 patients, 20 with cholelithiasis, 12 with chronic alcoholism, 24 with chronic gallstone pancreatitis and 30 with chronic alcoholic pancreatitis and 32 healthy subjects were served as controls. The patients with cholelithiasis showed exocrine hypersecretion of the pancreas. In the patients with gallstone pancreatitis, all the assessed parameters of exocrine function were depressed. On the other hand, no pancreatic exocrine dysfunction was dispalyed in cases with chronic alcoholism. In the non-calcifying alcoholic pancreatitis, both the volume output and the the maximum concentration and output of bicarbonate were diminished but depression in amylase output was not seen. All these parameters were lowered in patients with calcifying pancreatitis. Elevation of hexosamine concentration in the pancreatic juice was evident in alcoholic pancreatitis as compared with gallstone pancreatitis, being particularly prominent in cases of non-calcifying pancreatitis. Patients with alcoholic pancreatitis were observed to secrete viscous pancreatic juice richer in amylase and hexosamine content, than those in the patients with gallstone pancreatitis. Endocrine dysfunction of the pancreas is more frequent and intense in alcoholic pancreatitis than in gallstone pancreatitis.", "contents": "Comparison of pancreatic dysfunction in chronic alcoholic pancreatitis and chronic gallstone pancreatitis. Comparative assessments of the endocrine and exocrine functions of the pancreas associated with gallstone pancreatitis or alcoholic pancreatitis were performed in a series of 86 patients, 20 with cholelithiasis, 12 with chronic alcoholism, 24 with chronic gallstone pancreatitis and 30 with chronic alcoholic pancreatitis and 32 healthy subjects were served as controls. The patients with cholelithiasis showed exocrine hypersecretion of the pancreas. In the patients with gallstone pancreatitis, all the assessed parameters of exocrine function were depressed. On the other hand, no pancreatic exocrine dysfunction was dispalyed in cases with chronic alcoholism. In the non-calcifying alcoholic pancreatitis, both the volume output and the the maximum concentration and output of bicarbonate were diminished but depression in amylase output was not seen. All these parameters were lowered in patients with calcifying pancreatitis. Elevation of hexosamine concentration in the pancreatic juice was evident in alcoholic pancreatitis as compared with gallstone pancreatitis, being particularly prominent in cases of non-calcifying pancreatitis. Patients with alcoholic pancreatitis were observed to secrete viscous pancreatic juice richer in amylase and hexosamine content, than those in the patients with gallstone pancreatitis. Endocrine dysfunction of the pancreas is more frequent and intense in alcoholic pancreatitis than in gallstone pancreatitis.", "PMID": 976691}
{"id": "pubmed23n0032_10817", "title": "The discrepancy between plasma clearance tests of indocyanine green (ICG) and sulfobromophthalein (BSP): --report of cases and a study of ICG-binding pattern of serum proteins.", "content": "Two cases of Gilbert's syndrome and three cases of other diseases showed abnormally low plasma indocyanine green (ICG) clearance as contrated with normal or nearly normal plasma sulfobromphthalein (BSP) clearance. To evaluate the role of serum proteins in pathogenesis of these cases, ICG-binding patterns of serum proteins of the patients were compared with that of normal persons by the method of Sephadex G200 column chromatography. No qualitative differences in binding patterns were observed between serum proteins of the patients and normal persons. From these results, serum proteins were thought to be minor determinants of the hepatic ICG uptake mechanism. Because of normal or nearly normal plasma BSP clearance in these patients, the contents of hepatic organic anion-binding proteins, Y and Z, were thought to be sufficient. Possible qualitative abnormalities of the Y and/or Z proteins were also discussed.", "contents": "The discrepancy between plasma clearance tests of indocyanine green (ICG) and sulfobromophthalein (BSP): --report of cases and a study of ICG-binding pattern of serum proteins. Two cases of Gilbert's syndrome and three cases of other diseases showed abnormally low plasma indocyanine green (ICG) clearance as contrated with normal or nearly normal plasma sulfobromphthalein (BSP) clearance. To evaluate the role of serum proteins in pathogenesis of these cases, ICG-binding patterns of serum proteins of the patients were compared with that of normal persons by the method of Sephadex G200 column chromatography. No qualitative differences in binding patterns were observed between serum proteins of the patients and normal persons. From these results, serum proteins were thought to be minor determinants of the hepatic ICG uptake mechanism. Because of normal or nearly normal plasma BSP clearance in these patients, the contents of hepatic organic anion-binding proteins, Y and Z, were thought to be sufficient. Possible qualitative abnormalities of the Y and/or Z proteins were also discussed.", "PMID": 976692}
{"id": "pubmed23n0032_10818", "title": "[Altitude adaptation. Part II. Altitude adaptation as a problem of human biology. I. Growth)].", "content": "There are 3 main components influencing the mean stature of high altitude natives. The lower mean birthweight, which seems to result from the impossibility of complete adaptation during the last trimenon of gestation, which cannot be fully compensated during life. The general protein deficiency, or the habits of nutrition which are not comparable with the food-composition in industry-nations may be important. A definitive undernourishment has not been observed in those nations with longtime adaptation to the available food since generations, but several deficiencies of substances which specially influence growth, cannot be outruled. We also have to recognize the generally lower mean stature of the population in whole (South America Indians), that means of the both parents of a child. In the presence of low gene flow from one to the other generation this may influence also individual body measurement.", "contents": "[Altitude adaptation. Part II. Altitude adaptation as a problem of human biology. I. Growth)]. There are 3 main components influencing the mean stature of high altitude natives. The lower mean birthweight, which seems to result from the impossibility of complete adaptation during the last trimenon of gestation, which cannot be fully compensated during life. The general protein deficiency, or the habits of nutrition which are not comparable with the food-composition in industry-nations may be important. A definitive undernourishment has not been observed in those nations with longtime adaptation to the available food since generations, but several deficiencies of substances which specially influence growth, cannot be outruled. We also have to recognize the generally lower mean stature of the population in whole (South America Indians), that means of the both parents of a child. In the presence of low gene flow from one to the other generation this may influence also individual body measurement.", "PMID": 976693}
{"id": "pubmed23n0032_10819", "title": "Stages of seminiferous epithelial cycle and relative duration of spermatogenic processes in the buffalo (Bos bubalus).", "content": "By using haematoxylin-eosin preparations, the seminiferous epithelial cycle (SEC) in the buffalo has been divided into 8 stages; stages 1, 2, 3, 4 and 8 have been subdivided. The percentage frequency of these stages and substages has been determined by using the serial sections. The approximate relative duration for the various spermatogenic cells and processes has been worked out from the percentage frequency of a substage or a stage, and the number of substages or stages in which the particular cell or the cell phase was present. The total approximate duration of spermatogenesis in the buffalo is 457 units, which has been divided by the duration of one SEC (i.e. 100 units). The buffalo spermatogenesis is constituted by 4.57 cycles of seminiferous epithelium. The results of the present studies have been compared and contrasted with those of previous studies on various mammalian species including farm animals. For its kinetics of spermatogenesis, the buffalo resembles the bull closely.", "contents": "Stages of seminiferous epithelial cycle and relative duration of spermatogenic processes in the buffalo (Bos bubalus). By using haematoxylin-eosin preparations, the seminiferous epithelial cycle (SEC) in the buffalo has been divided into 8 stages; stages 1, 2, 3, 4 and 8 have been subdivided. The percentage frequency of these stages and substages has been determined by using the serial sections. The approximate relative duration for the various spermatogenic cells and processes has been worked out from the percentage frequency of a substage or a stage, and the number of substages or stages in which the particular cell or the cell phase was present. The total approximate duration of spermatogenesis in the buffalo is 457 units, which has been divided by the duration of one SEC (i.e. 100 units). The buffalo spermatogenesis is constituted by 4.57 cycles of seminiferous epithelium. The results of the present studies have been compared and contrasted with those of previous studies on various mammalian species including farm animals. For its kinetics of spermatogenesis, the buffalo resembles the bull closely.", "PMID": 976694}
{"id": "pubmed23n0032_10820", "title": "[Quantitative description of growth processes in man as a basis for the objective comparison of sex-specific data for various body measurements].", "content": "To compare growth processes of feminine human beings with such one of masculine we have taken as realizations of the growth characteristic the morphological height of face (distance between nasion and gnathion), length of nose (distance between nasion and subnasale) and body length of two spatially and temporally different populations. Measured values of these characteristics for different ages of individuals provide the basis for mathematical evaluation. The parameters of the following three types of mathematical functions were adjusted to the measured time course of the growth characteristics: Gompertz' function, inverse tangent and the generalized logistic growth function. The principal possibilities are explained which a mathematical expression can offer for qualitative and quantitative description of dynamical processes. The results of fitting the 3 competitive function types to the course of the measured values are given and several quantitative conclusions are demonstrated arising from comparisons of corresponding growth processes of feminine and masculine human beings.", "contents": "[Quantitative description of growth processes in man as a basis for the objective comparison of sex-specific data for various body measurements]. To compare growth processes of feminine human beings with such one of masculine we have taken as realizations of the growth characteristic the morphological height of face (distance between nasion and gnathion), length of nose (distance between nasion and subnasale) and body length of two spatially and temporally different populations. Measured values of these characteristics for different ages of individuals provide the basis for mathematical evaluation. The parameters of the following three types of mathematical functions were adjusted to the measured time course of the growth characteristics: Gompertz' function, inverse tangent and the generalized logistic growth function. The principal possibilities are explained which a mathematical expression can offer for qualitative and quantitative description of dynamical processes. The results of fitting the 3 competitive function types to the course of the measured values are given and several quantitative conclusions are demonstrated arising from comparisons of corresponding growth processes of feminine and masculine human beings.", "PMID": 976695}
{"id": "pubmed23n0032_10821", "title": "[The origin and ramifications of the intracavernous section of the internal carotid artery].", "content": "The fine ramifications of the internal carotid artery arising in the cavernous sinus were minutely prepared and investigated on 23 heads: 1) Origins, branchings and their variations have been systematically traced. 2) The areas supplied by the carotico-cavernous ramifications of the internal carotid artery their anastomoses and positioning to one another have been analysed and classified. 3) Three schematic illustrations prepared from the specimens dissected summarize and annotate the findings. 4) The diagrams have also been included which summa iza and compare the results of earlier investigators. The newly discovered and heretofore unpublished variations have been included along with a comparison to these of the already varying terminology extant for the origins, branchings, final destinations and relationships.", "contents": "[The origin and ramifications of the intracavernous section of the internal carotid artery]. The fine ramifications of the internal carotid artery arising in the cavernous sinus were minutely prepared and investigated on 23 heads: 1) Origins, branchings and their variations have been systematically traced. 2) The areas supplied by the carotico-cavernous ramifications of the internal carotid artery their anastomoses and positioning to one another have been analysed and classified. 3) Three schematic illustrations prepared from the specimens dissected summarize and annotate the findings. 4) The diagrams have also been included which summa iza and compare the results of earlier investigators. The newly discovered and heretofore unpublished variations have been included along with a comparison to these of the already varying terminology extant for the origins, branchings, final destinations and relationships.", "PMID": 976696}
{"id": "pubmed23n0032_10822", "title": "Structural observations on the gills of two fresh water teleost fishes, Notopterus notopterus and Colisa fasciatus.", "content": "1. The structural observations of the gills of Notopterus and Colisa fasciatus have been studied in detail. 2. The gills of Notopterus notopterus and Colisa fasciatus consist of 2 regions: a) Gill head region, and b) Gill lamellae region. Gill head region is covered by epithelial layer possessing the mucous and mast cells. Gill head region consists of bony arch, gill rakers and interbranchial septum. 3. Interbranchial septum is reduced in Notopterus notopterus while it extends near half to the length of the filament in Colisa fasciatus. 4. In both fishes, the adductor muscle, afferent and efferent branchial vessels and gill rays are present in the interbranchial septum area. 5. Gill lamellae region consists of primary and secondary lamellae in both the fishes. 6. In Notopterus notopterus the primary lamellae are compressed flat structures possessing gill rays, 1 afferent and 1 efferent branchial vessel, while in Colisa fasciatus 2 efferent vessels are present. 7. Secondary lamellae are arranged parallel to one another on both sides of the primary lamellae. Secondary lamellae consist of basement membrane, epithelial cells, vascular layer and pillar cells. Mucous and mast cells are also present in this region in case of Notopterus notopterus while only mast cells are present in Colisa fasciatus. 8. Mast cells are eosinophilic, highly granulated in both the fishes.", "contents": "Structural observations on the gills of two fresh water teleost fishes, Notopterus notopterus and Colisa fasciatus. 1. The structural observations of the gills of Notopterus and Colisa fasciatus have been studied in detail. 2. The gills of Notopterus notopterus and Colisa fasciatus consist of 2 regions: a) Gill head region, and b) Gill lamellae region. Gill head region is covered by epithelial layer possessing the mucous and mast cells. Gill head region consists of bony arch, gill rakers and interbranchial septum. 3. Interbranchial septum is reduced in Notopterus notopterus while it extends near half to the length of the filament in Colisa fasciatus. 4. In both fishes, the adductor muscle, afferent and efferent branchial vessels and gill rays are present in the interbranchial septum area. 5. Gill lamellae region consists of primary and secondary lamellae in both the fishes. 6. In Notopterus notopterus the primary lamellae are compressed flat structures possessing gill rays, 1 afferent and 1 efferent branchial vessel, while in Colisa fasciatus 2 efferent vessels are present. 7. Secondary lamellae are arranged parallel to one another on both sides of the primary lamellae. Secondary lamellae consist of basement membrane, epithelial cells, vascular layer and pillar cells. Mucous and mast cells are also present in this region in case of Notopterus notopterus while only mast cells are present in Colisa fasciatus. 8. Mast cells are eosinophilic, highly granulated in both the fishes.", "PMID": 976698}
{"id": "pubmed23n0032_10823", "title": "[Methods for approximate and exact complanation of spheroidal-cell nuclei as an aid for statistical karyometric studies on nuclear variation].", "content": "After short mathematical explanations on exact formulas for surface area calculation of the three-axis ellipsoid and the spheroid, approximation formulas, which for the most part are modified formulas of the spherical surface area, are developed. By application of of Jacobj's linear method and Lange's curvimeter-planimeter method, these approximation formulas are used to calculate the surface areas with identical semi-axis and diameter values being inserted, and the results are compared with each other and the true values. If the design procedure is extended to other axial rations, numeric and per cent surveys result in the form of tables. The approximation formulas, whose results differ only a little from the true value and which require only a minimum of computing effort are selected. For the three-axis ellipsoid as well as for the spheroid, the true surface area values can be determined from the approximate value, with help of tabulated percentages and by interpolation of intermediate values are required. Interpolation formulas have been formulated, for that purpose, in addition. In the last part an exact formula for the nucleus surface area, if it has the shape of a spheroid, has been developed. According to the formula there exists a dependence of nucleus volume and axial ratio as the basis of a mixed cyclometric function. A further detailed table contains these surface area values, arranged in groups of equal axial ratios. In addition two other formulas have been evolved for exact computing of the nucleus surface area. For one it is dependent on the two diameters and the mixed cyclometric function which is based on the axial ratio; for the other it depends on the large diameter, the axial ratio and the mixed cyclometric function. Numerical examples have been worked out for all representations. For u = n = const, the surface values from terms of an arithmetical second-order procession. In case of equal axial ratios a recurrence formula can be worked out, according to which the succeeding term can be calculated from its predecessor.", "contents": "[Methods for approximate and exact complanation of spheroidal-cell nuclei as an aid for statistical karyometric studies on nuclear variation]. After short mathematical explanations on exact formulas for surface area calculation of the three-axis ellipsoid and the spheroid, approximation formulas, which for the most part are modified formulas of the spherical surface area, are developed. By application of of Jacobj's linear method and Lange's curvimeter-planimeter method, these approximation formulas are used to calculate the surface areas with identical semi-axis and diameter values being inserted, and the results are compared with each other and the true values. If the design procedure is extended to other axial rations, numeric and per cent surveys result in the form of tables. The approximation formulas, whose results differ only a little from the true value and which require only a minimum of computing effort are selected. For the three-axis ellipsoid as well as for the spheroid, the true surface area values can be determined from the approximate value, with help of tabulated percentages and by interpolation of intermediate values are required. Interpolation formulas have been formulated, for that purpose, in addition. In the last part an exact formula for the nucleus surface area, if it has the shape of a spheroid, has been developed. According to the formula there exists a dependence of nucleus volume and axial ratio as the basis of a mixed cyclometric function. A further detailed table contains these surface area values, arranged in groups of equal axial ratios. In addition two other formulas have been evolved for exact computing of the nucleus surface area. For one it is dependent on the two diameters and the mixed cyclometric function which is based on the axial ratio; for the other it depends on the large diameter, the axial ratio and the mixed cyclometric function. Numerical examples have been worked out for all representations. For u = n = const, the surface values from terms of an arithmetical second-order procession. In case of equal axial ratios a recurrence formula can be worked out, according to which the succeeding term can be calculated from its predecessor.", "PMID": 976699}
{"id": "pubmed23n0032_10824", "title": "[Passive electric studies on the normal and atherosclerotic human aorta at ultra-low frequencies].", "content": "The passive electrical behaviour of the normal and the atherosclerotic human aorta was investigated at very low frequencies between 0.4 and 0.002 Hz. In the normal as well as in the pathologically changed vessel wall the upper part of an additional dispersion area below the region of alpha-dispersion was found. Furthermore all values of the dielectric constant in atheromatous plaques were situated above the values of the normal aorta. There is every indication that also at very low frequencies the dispersion behaviour of the atheromatous plaques is determined by larger structures than in the normal aorta. The further extension of the measurements to even lower frequencies and theraby the registration of the total dispersion area is limited by the inconstancy of the measuring object during a period (about 15 min).", "contents": "[Passive electric studies on the normal and atherosclerotic human aorta at ultra-low frequencies]. The passive electrical behaviour of the normal and the atherosclerotic human aorta was investigated at very low frequencies between 0.4 and 0.002 Hz. In the normal as well as in the pathologically changed vessel wall the upper part of an additional dispersion area below the region of alpha-dispersion was found. Furthermore all values of the dielectric constant in atheromatous plaques were situated above the values of the normal aorta. There is every indication that also at very low frequencies the dispersion behaviour of the atheromatous plaques is determined by larger structures than in the normal aorta. The further extension of the measurements to even lower frequencies and theraby the registration of the total dispersion area is limited by the inconstancy of the measuring object during a period (about 15 min).", "PMID": 976700}
{"id": "pubmed23n0032_10825", "title": "Quantitative analysis of the growth of the myotomes of freshwater teleost, Ophicephalus punctatus Bloch (= Channa punctata).", "content": "A positive correlation was found to exist between the growth of the myotomes, in thickness, and that of the body length of Ophicephalus punctatus Bloch, a common freshwater teleost. The changes in the absolute and the relative growth of the myotomes, as observed in this fish, were found to be age-specific. The constancy in the mysosomatic indices as also in the ratios of the differential growth rates of the myotomes did not seem to permit any divergence from the linear relation between the growth of the body and the myotomes.", "contents": "Quantitative analysis of the growth of the myotomes of freshwater teleost, Ophicephalus punctatus Bloch (= Channa punctata). A positive correlation was found to exist between the growth of the myotomes, in thickness, and that of the body length of Ophicephalus punctatus Bloch, a common freshwater teleost. The changes in the absolute and the relative growth of the myotomes, as observed in this fish, were found to be age-specific. The constancy in the mysosomatic indices as also in the ratios of the differential growth rates of the myotomes did not seem to permit any divergence from the linear relation between the growth of the body and the myotomes.", "PMID": 976701}
{"id": "pubmed23n0032_10826", "title": "[The jet-wash-technique (author's transl)].", "content": "For the early detection of the endometrial adenocarcinoma the Gravlee-Jet-wash-technique was used in 600 cases. The procedure is simple and requires only 2 to 3 minutes to the gynecologist. An anesthesia is not necessary. In women with stenosed os externum and in anxious patients we use an analgesia of the paracervix (PCB). The technique allows a cytologic and/or cytohistologic investigation. In 600 examined cases we found 37 endometrial adenocarcinomas. We obtained a 100 per cent accuracy in the detection of the 37 malignant lesions. Our experience up to now with the Jet-wash-technique should stimulate its wider use also for outpatients with a high risk for the endometrial adenocarcinoma.", "contents": "[The jet-wash-technique (author's transl)]. For the early detection of the endometrial adenocarcinoma the Gravlee-Jet-wash-technique was used in 600 cases. The procedure is simple and requires only 2 to 3 minutes to the gynecologist. An anesthesia is not necessary. In women with stenosed os externum and in anxious patients we use an analgesia of the paracervix (PCB). The technique allows a cytologic and/or cytohistologic investigation. In 600 examined cases we found 37 endometrial adenocarcinomas. We obtained a 100 per cent accuracy in the detection of the 37 malignant lesions. Our experience up to now with the Jet-wash-technique should stimulate its wider use also for outpatients with a high risk for the endometrial adenocarcinoma.", "PMID": 976702}
{"id": "pubmed23n0032_10827", "title": "[Results of the conservative management of carcinoma in situ of the uterine cervix by conization (author's transl)].", "content": "From 1958 to 1969 conizations for carcinoma in situ of the uterine cervix were carried out in 683 women. In 523 cases no further therapy was instituted immediately. Step serial sections of the conization specimens showed that carcinoma in situ was completely removed in 346 (66.2%) of the cases. In 177 (33.8%) of the cases, the excision of the atypical epithelium was incomplete or not with certainty complete, by the conization. Of these patients 43 women were older than 45 years. Follow-up examinations after conization with complete removal of all atypical epithelium showed that only one case showed again a pathological epithelium. In this case, hysterectomy for an adnexal tumor four years after conization showed a mild dysplasia of the endocervix. Follow-up examination of the cases with incomplete or possibly incomplete conization showed no further pathological finding in 90% of the cases. In 19 (10.7%) of the women, atypical epithelium reappeared after varying lengths of follow-up. In 4 women, invasive carcinoma had developed and in 2 women, microinvasive carcinoma had developed. Following operative treatment, further follow-up examinations in these patients were negative. Conization of the uterine cervix with complete removal of all atypical epithelium is shown to be adequate treatment for carcinoma in situ. In cases with incomplete or not with certainty complete removal of atypical tissue by conization, individual follow-up is necessary. If further pregnancies are desired the small risk of observation is justifiable. Diagnosed persistence of atypical epithelium is an indication for a hysterectomy. Hysterectomies are also necessary if the patient does not comply adequately with the follow-up protocol.", "contents": "[Results of the conservative management of carcinoma in situ of the uterine cervix by conization (author's transl)]. From 1958 to 1969 conizations for carcinoma in situ of the uterine cervix were carried out in 683 women. In 523 cases no further therapy was instituted immediately. Step serial sections of the conization specimens showed that carcinoma in situ was completely removed in 346 (66.2%) of the cases. In 177 (33.8%) of the cases, the excision of the atypical epithelium was incomplete or not with certainty complete, by the conization. Of these patients 43 women were older than 45 years. Follow-up examinations after conization with complete removal of all atypical epithelium showed that only one case showed again a pathological epithelium. In this case, hysterectomy for an adnexal tumor four years after conization showed a mild dysplasia of the endocervix. Follow-up examination of the cases with incomplete or possibly incomplete conization showed no further pathological finding in 90% of the cases. In 19 (10.7%) of the women, atypical epithelium reappeared after varying lengths of follow-up. In 4 women, invasive carcinoma had developed and in 2 women, microinvasive carcinoma had developed. Following operative treatment, further follow-up examinations in these patients were negative. Conization of the uterine cervix with complete removal of all atypical epithelium is shown to be adequate treatment for carcinoma in situ. In cases with incomplete or not with certainty complete removal of atypical tissue by conization, individual follow-up is necessary. If further pregnancies are desired the small risk of observation is justifiable. Diagnosed persistence of atypical epithelium is an indication for a hysterectomy. Hysterectomies are also necessary if the patient does not comply adequately with the follow-up protocol.", "PMID": 976703}
{"id": "pubmed23n0032_10828", "title": "[Adequate treatment of micro-invasive carcinoma of the uterine cervix (author's transl)].", "content": "At the University Department in Heidelberg 58 micro-invasive carcinomas of the uterine cervix were found between 1965 and 1974. 8 cases were diagnosed in other institutions by directed biopsies. Three cases were detected during the microscopic examination of uteri removed for emergency indications and three cases were detected after hysterectomy for cytological findings indicative of carcinoma in situ of the uterine cervix. In 44 cases an optimal conization of the cervix was carried out. Microscopic examination of these cases showed early stromal invasion in 33 cases. In 6 cases a newtork infiltration was found und in 5 cases a plump infiltration was found. The incidence of radical Wertheim operations of 21 in this group is explained by cases in this group who had inadequate diagnosis prior to admission. In 8 cases the indication for the Wertheim radical hysterectomy was a carcinomatous lymphangiosis in the conization specimen. In none of these cases metastatic tumor was found in the lymph nodes although 13 to 44 lymph nodes were examined per case. In accordance with the newer literature it is recommended to limit treatment of micro-invasive changes of the uterine cervix to routine hysterectomy or to optimal conization of the cervix if certain prerequisites are met. The results of the follow-up examinations of the reported cases appear to justify this recommendation.", "contents": "[Adequate treatment of micro-invasive carcinoma of the uterine cervix (author's transl)]. At the University Department in Heidelberg 58 micro-invasive carcinomas of the uterine cervix were found between 1965 and 1974. 8 cases were diagnosed in other institutions by directed biopsies. Three cases were detected during the microscopic examination of uteri removed for emergency indications and three cases were detected after hysterectomy for cytological findings indicative of carcinoma in situ of the uterine cervix. In 44 cases an optimal conization of the cervix was carried out. Microscopic examination of these cases showed early stromal invasion in 33 cases. In 6 cases a newtork infiltration was found und in 5 cases a plump infiltration was found. The incidence of radical Wertheim operations of 21 in this group is explained by cases in this group who had inadequate diagnosis prior to admission. In 8 cases the indication for the Wertheim radical hysterectomy was a carcinomatous lymphangiosis in the conization specimen. In none of these cases metastatic tumor was found in the lymph nodes although 13 to 44 lymph nodes were examined per case. In accordance with the newer literature it is recommended to limit treatment of micro-invasive changes of the uterine cervix to routine hysterectomy or to optimal conization of the cervix if certain prerequisites are met. The results of the follow-up examinations of the reported cases appear to justify this recommendation.", "PMID": 976704}
{"id": "pubmed23n0032_10829", "title": "[The low dosis gestagen therapy (author's transl)].", "content": "In young women aged 22-34 years the hormone profile under continuous therapy with 0.5 mg lynestrenol/day have been investigated. Estradiol, progesterone and FSH values have been determined in plasma by the RIA or protein-binding method, whereas LH excretion was daily measured in the urine starting on the 5th day of cycle. The low-dose-gestagen therapy shows all possible hormonal patterns ranging from the ovulatory to anovulatory one. The estradiol-17 B values in half of the patients were higher than in the cycle preceding treatment. In the first few treatment cycles all women showed markedly decreased progesterone values. However, this defect seems to recover and return to normal in later cycles. In order to be able to further explain the alterations occurring during the luteal phase under the treatment with 0.5 mg/day of lynestrenol, ovarian tissues wedge resection of women undergoing gynecological surgery has been examined macroscopically and microscopically. At the same time the hormonal profile has been determined in plasma. These investigations revealed that the morphological alterations in the ovary proceed in an unchanged way under the mentioned treatment. Histologically it could be demonstrated that ovulation is delayed by several days. The histological findings of tubal tissue (from patients who underwent sterilization) showed that the secretional activity of the tubal epithelium is considerably decreased and ciliogenesis increased. In our clinical study neither tablet nor patient failures occurred. With the exception of cycle- and tempo-disturbances no severe adverse reactions were reported. Breakthrough bleedings and spotting as well as amenorrhea in most cases manifested already in the first treatment cycles. It is important that the daily tablet is taken at midday because the maximum effect on the cervical mucus of the mini-pill therapy is most pronounced 4-6 hours after tablet intake. Especially in young patients it is recommended to get written reports on tablet intake. The strict following of these directions mainly guarantees a successful mini-pill therapy.", "contents": "[The low dosis gestagen therapy (author's transl)]. In young women aged 22-34 years the hormone profile under continuous therapy with 0.5 mg lynestrenol/day have been investigated. Estradiol, progesterone and FSH values have been determined in plasma by the RIA or protein-binding method, whereas LH excretion was daily measured in the urine starting on the 5th day of cycle. The low-dose-gestagen therapy shows all possible hormonal patterns ranging from the ovulatory to anovulatory one. The estradiol-17 B values in half of the patients were higher than in the cycle preceding treatment. In the first few treatment cycles all women showed markedly decreased progesterone values. However, this defect seems to recover and return to normal in later cycles. In order to be able to further explain the alterations occurring during the luteal phase under the treatment with 0.5 mg/day of lynestrenol, ovarian tissues wedge resection of women undergoing gynecological surgery has been examined macroscopically and microscopically. At the same time the hormonal profile has been determined in plasma. These investigations revealed that the morphological alterations in the ovary proceed in an unchanged way under the mentioned treatment. Histologically it could be demonstrated that ovulation is delayed by several days. The histological findings of tubal tissue (from patients who underwent sterilization) showed that the secretional activity of the tubal epithelium is considerably decreased and ciliogenesis increased. In our clinical study neither tablet nor patient failures occurred. With the exception of cycle- and tempo-disturbances no severe adverse reactions were reported. Breakthrough bleedings and spotting as well as amenorrhea in most cases manifested already in the first treatment cycles. It is important that the daily tablet is taken at midday because the maximum effect on the cervical mucus of the mini-pill therapy is most pronounced 4-6 hours after tablet intake. Especially in young patients it is recommended to get written reports on tablet intake. The strict following of these directions mainly guarantees a successful mini-pill therapy.", "PMID": 976705}
{"id": "pubmed23n0032_10830", "title": "[The optimal caesarean section rate in breech deliveries (author's transl)].", "content": "A critical review of 535 breech deliveries over 15 years is presented and compared to the present trend to deliver all primigravida breech presentations by Caesarean Section. Management of the labour and delivery in view of a vaginal delivery was started in 84% of the cases. The Caesarean Section rate was 16%. The prenatal mortality compared well to that reported in the literature. The relatively high total morbidity of 3.2% showed the increased risk for the infant of the primigravida (1.9%) and of the multigravida (1.7%). 1.5% of the infants were transferred to the pediatric department because of birth trauma. All children with birth trauma were delivered prior to the increase of the Caesarean Section rate from 9.2% to 21.2%. Since 1969 the fetal morbidity was 0% for breech deliveries.", "contents": "[The optimal caesarean section rate in breech deliveries (author's transl)]. A critical review of 535 breech deliveries over 15 years is presented and compared to the present trend to deliver all primigravida breech presentations by Caesarean Section. Management of the labour and delivery in view of a vaginal delivery was started in 84% of the cases. The Caesarean Section rate was 16%. The prenatal mortality compared well to that reported in the literature. The relatively high total morbidity of 3.2% showed the increased risk for the infant of the primigravida (1.9%) and of the multigravida (1.7%). 1.5% of the infants were transferred to the pediatric department because of birth trauma. All children with birth trauma were delivered prior to the increase of the Caesarean Section rate from 9.2% to 21.2%. Since 1969 the fetal morbidity was 0% for breech deliveries.", "PMID": 976706}
{"id": "pubmed23n0032_10831", "title": "[Sexual education and contentment with the feminine sexual role (author's transl)].", "content": "The correlation of sexual education during childhood and the later contentment with the feminine sexual role was quantitively tested for its statistical significance. The data were collected for 386 women with a questionnaire and psychometric testing. Contentment with the feminine sexual role showed correlations to the age of the first sexual education (p smaller than 0.01), to the quality of the sexual education (p smaller than 0.05) and to the experience of the individual sexuality during adolescence (p smaller than 0.05). The earlier and the more positive the sex education the higher was the contentment with the own sexual role. Women who were most content with their presently experienced sexuality were also most content with their social role (p smaller than 0.01). Of importance in the discussion of the results were relationships between the contentment with the sexual role and the age (p smaller than 0.05), neurotic personality traits (p smaller than 0.01), obesity (p smaller than 0.01), psychosomatic complaints (p smaller than 0.05) and the present state of health (p smaller than 0.001). These results are of practical importance for sexual councelling in gynaecology.", "contents": "[Sexual education and contentment with the feminine sexual role (author's transl)]. The correlation of sexual education during childhood and the later contentment with the feminine sexual role was quantitively tested for its statistical significance. The data were collected for 386 women with a questionnaire and psychometric testing. Contentment with the feminine sexual role showed correlations to the age of the first sexual education (p smaller than 0.01), to the quality of the sexual education (p smaller than 0.05) and to the experience of the individual sexuality during adolescence (p smaller than 0.05). The earlier and the more positive the sex education the higher was the contentment with the own sexual role. Women who were most content with their presently experienced sexuality were also most content with their social role (p smaller than 0.01). Of importance in the discussion of the results were relationships between the contentment with the sexual role and the age (p smaller than 0.05), neurotic personality traits (p smaller than 0.01), obesity (p smaller than 0.01), psychosomatic complaints (p smaller than 0.05) and the present state of health (p smaller than 0.001). These results are of practical importance for sexual councelling in gynaecology.", "PMID": 976707}
{"id": "pubmed23n0032_10832", "title": "[Rokitansky-K\u00fcstner syndrome (absent vagina, rudimentary uterus) associated with renal aplasia, phokomelia and Klippel-Feil anomaly (author's transl)].", "content": "A case of multiple malformations, absent vagina, rudimentary uterus (Rokitansky-K\u00fcstner syndrome), renal aplasia, fusion of the cervical vertebral bones (Klippel-Feil anomaly) and phokomelia is presented. Association of absent vagina and Klippel-Feil anomaly is variously reported, however in this case these anomalies are associated with phokomelia. Etiology of these malformations is discussed with the view to similarity to the Thalidomide syndrome.", "contents": "[Rokitansky-K\u00fcstner syndrome (absent vagina, rudimentary uterus) associated with renal aplasia, phokomelia and Klippel-Feil anomaly (author's transl)]. A case of multiple malformations, absent vagina, rudimentary uterus (Rokitansky-K\u00fcstner syndrome), renal aplasia, fusion of the cervical vertebral bones (Klippel-Feil anomaly) and phokomelia is presented. Association of absent vagina and Klippel-Feil anomaly is variously reported, however in this case these anomalies are associated with phokomelia. Etiology of these malformations is discussed with the view to similarity to the Thalidomide syndrome.", "PMID": 976708}
{"id": "pubmed23n0032_10833", "title": "[Histological changes in the ligated vasa ovarica (author's transl)].", "content": "During gynecological operations 45 ligamenta infundibulopelvica were resected after 5 to 45 minutes. The intravascular changes of the ligated vasa ovarica were examinated. The histological examinations of the stumps showed, that the organism protects itself by formation of obstruction thrombi against a hemorrhage. The blood coagulation processes are local limited and the endothelial damages are causal important for the obstruction and obliteration.", "contents": "[Histological changes in the ligated vasa ovarica (author's transl)]. During gynecological operations 45 ligamenta infundibulopelvica were resected after 5 to 45 minutes. The intravascular changes of the ligated vasa ovarica were examinated. The histological examinations of the stumps showed, that the organism protects itself by formation of obstruction thrombi against a hemorrhage. The blood coagulation processes are local limited and the endothelial damages are causal important for the obstruction and obliteration.", "PMID": 976709}
{"id": "pubmed23n0032_10834", "title": "[Premature infant with traumatic loss of the left arm and shoulder caused by an ineffective interruption of pregnancy (author's transl)].", "content": "A case of a premature infant with traumatic loss of the left arm and shoulder is reported. The trauma was caused by an ineffective interruption of pregnancy.", "contents": "[Premature infant with traumatic loss of the left arm and shoulder caused by an ineffective interruption of pregnancy (author's transl)]. A case of a premature infant with traumatic loss of the left arm and shoulder is reported. The trauma was caused by an ineffective interruption of pregnancy.", "PMID": 976711}
{"id": "pubmed23n0032_10835", "title": "[The vaginal transverse low segment hysterotomy for second trimester therapeutic abortion (author's transl)].", "content": "A series of 100 therapeutic abortions by vaginal hysterotomy is reported. This method of vaginal hysterotomy with a transverse low segment incision just above the internal os of the cervix was first described by Fuchs in 1939. The important advantage of this method is complete avoidance of the uterine cervix. With this method a therapeutic abortion after the tenth week of gestation and in a few cases, up to the 20th week of gestation can be accomplished by a fast one stage technique with few side effects. The method is considered to be superior to the injection methods and the two stage procedures. With skill in vaginal operative techniques, this method can be used in any gynaecological department as a routine method. In this series, 28 cases had concomitant tubal ligations. There were two subsequent pregnancies. Complications were one uterine perforation during the curettage requiring laparotomy and suture. There was one laceration of the trigon of the bladder requiring suture. There were 2 cases of hemorrhage. There were 6 inflammatory reactions in the perimetria of which two required administration of antibiotics. There was superficial thrombophlebitis in one case. And there was a severe coagulation defect in one case of a missed abortion at 5 months gestation.", "contents": "[The vaginal transverse low segment hysterotomy for second trimester therapeutic abortion (author's transl)]. A series of 100 therapeutic abortions by vaginal hysterotomy is reported. This method of vaginal hysterotomy with a transverse low segment incision just above the internal os of the cervix was first described by Fuchs in 1939. The important advantage of this method is complete avoidance of the uterine cervix. With this method a therapeutic abortion after the tenth week of gestation and in a few cases, up to the 20th week of gestation can be accomplished by a fast one stage technique with few side effects. The method is considered to be superior to the injection methods and the two stage procedures. With skill in vaginal operative techniques, this method can be used in any gynaecological department as a routine method. In this series, 28 cases had concomitant tubal ligations. There were two subsequent pregnancies. Complications were one uterine perforation during the curettage requiring laparotomy and suture. There was one laceration of the trigon of the bladder requiring suture. There were 2 cases of hemorrhage. There were 6 inflammatory reactions in the perimetria of which two required administration of antibiotics. There was superficial thrombophlebitis in one case. And there was a severe coagulation defect in one case of a missed abortion at 5 months gestation.", "PMID": 976712}
{"id": "pubmed23n0032_10836", "title": "[Methods of artificial termination of pregnancy with special consideration of the use of prostaglandin (author's transl)].", "content": "For termination of a pregnancy up until the 12th week, the method of choice is suction curettage. The dilation of the cervical canal is made easier and sometimes the embryo is aborted if a viscid preparation containing Prostaglandin F 22 is injected intracervically 12 hours prior to the scheduled procedure. For termination of pregnancy after the 14th week, prostaglandin is superior to all other methods. It can be used intraamnially, extra-amnially or intravenously. Not enough information and experience is available in Europe regarding the intravaginal application.", "contents": "[Methods of artificial termination of pregnancy with special consideration of the use of prostaglandin (author's transl)]. For termination of a pregnancy up until the 12th week, the method of choice is suction curettage. The dilation of the cervical canal is made easier and sometimes the embryo is aborted if a viscid preparation containing Prostaglandin F 22 is injected intracervically 12 hours prior to the scheduled procedure. For termination of pregnancy after the 14th week, prostaglandin is superior to all other methods. It can be used intraamnially, extra-amnially or intravenously. Not enough information and experience is available in Europe regarding the intravaginal application.", "PMID": 976713}
{"id": "pubmed23n0032_10837", "title": "[Psychosocial problems of young patients with aplasia vaginae (author's transl)].", "content": "10 patients with aplasia vaginae et uteri (Mayer-Rokitansky-K\u00fcster-syndrome) were interviewed by 2 psychologically trained persons. Vaginoplasty had already been performed in 8 patients. For these patients, the time between their first consultation of a doctor and vaginoplasty had been rather difficult. During this time, they had the strong feeling of being different from other girls. This feeling was favoured by the lack of information about the kind of their anomaly and the operation. The preoperative well informed patients possibly tolerated the painful application of vaginal prosthesis better than others. All patients with aplasia vaginae et uteri had many questions concerning their exspected sexual relations. Their knowledge on sexual function was very poor. We think, that cooperation between gynecologist and psychologist or psychiatrist is necessary for good results in treatment of patients with the Mayer-Rokitansky-K\u00fcster-syndrome.", "contents": "[Psychosocial problems of young patients with aplasia vaginae (author's transl)]. 10 patients with aplasia vaginae et uteri (Mayer-Rokitansky-K\u00fcster-syndrome) were interviewed by 2 psychologically trained persons. Vaginoplasty had already been performed in 8 patients. For these patients, the time between their first consultation of a doctor and vaginoplasty had been rather difficult. During this time, they had the strong feeling of being different from other girls. This feeling was favoured by the lack of information about the kind of their anomaly and the operation. The preoperative well informed patients possibly tolerated the painful application of vaginal prosthesis better than others. All patients with aplasia vaginae et uteri had many questions concerning their exspected sexual relations. Their knowledge on sexual function was very poor. We think, that cooperation between gynecologist and psychologist or psychiatrist is necessary for good results in treatment of patients with the Mayer-Rokitansky-K\u00fcster-syndrome.", "PMID": 976714}
{"id": "pubmed23n0032_10838", "title": "[Prostaglandin-F-levels in human plasma during late pregnancy and labour (author's transl)].", "content": "The concentrations of prostaglandin F-equivalents were measured in peripheral plasma during labour at a cervical dilatation of 5 cm and at complete dilatation. After purification, extraction and chromatography the PGF-equivalents were measured radioimmunologically. The intraassay variation was 1.5%, the interassay variation 3.5%. The specificity for PGF was 96-98%. Logit/log transformation of the standardcurve yielded a sensitivity of the assay of 120 pg. At cervical dilatation of 5 cm PGF-equivalents varied between 1300 and 3200 pg/ml plasma. At complete dilatation values changed between 1200 and 5400 pg/ml. These fluctuations correlate timedepending to the uterine contractions recorded and may be interpreted as a result of uterine PGF-release.", "contents": "[Prostaglandin-F-levels in human plasma during late pregnancy and labour (author's transl)]. The concentrations of prostaglandin F-equivalents were measured in peripheral plasma during labour at a cervical dilatation of 5 cm and at complete dilatation. After purification, extraction and chromatography the PGF-equivalents were measured radioimmunologically. The intraassay variation was 1.5%, the interassay variation 3.5%. The specificity for PGF was 96-98%. Logit/log transformation of the standardcurve yielded a sensitivity of the assay of 120 pg. At cervical dilatation of 5 cm PGF-equivalents varied between 1300 and 3200 pg/ml plasma. At complete dilatation values changed between 1200 and 5400 pg/ml. These fluctuations correlate timedepending to the uterine contractions recorded and may be interpreted as a result of uterine PGF-release.", "PMID": 976715}
{"id": "pubmed23n0032_10839", "title": "[Does the site of the placenta in the area of the caudal section of the uterus change during pregnancy? An echographic observation of the course of pregnancy (author's transl)].", "content": "Via examinations by ultrasonic sectional view during the course of pregnancy, the growth of the placenta was followed in 52 pregnant women. When a pathologic location of the placenta could be excluded during the first examination in the second trimester (26 cases), secondary development of a pathologic insertion was not observed. In 26 cases in which a pathological location of the placenta could be established before the 20th gestation week, a cranial shift in the caudal placenta border of up to 8 cm could be established in 19 cases. A pathological location of the placenta diagnosed in the second trimester can disappear. The shape of the uterus and the alterations in the spacial relationship between placenta and internal os uteri were caused by the development and stretching of the lower segment of the uterus. The vaginal hemorrhages in the second trimester caused by a low placental insertion (13 cases) stopped (with the exception of 2 cases) at the latest after the 6th month of pregnancy. The definitive establishment of a placenta pravia before the 5th-6th month of pregnancy is virtually impossible sonographically.", "contents": "[Does the site of the placenta in the area of the caudal section of the uterus change during pregnancy? An echographic observation of the course of pregnancy (author's transl)]. Via examinations by ultrasonic sectional view during the course of pregnancy, the growth of the placenta was followed in 52 pregnant women. When a pathologic location of the placenta could be excluded during the first examination in the second trimester (26 cases), secondary development of a pathologic insertion was not observed. In 26 cases in which a pathological location of the placenta could be established before the 20th gestation week, a cranial shift in the caudal placenta border of up to 8 cm could be established in 19 cases. A pathological location of the placenta diagnosed in the second trimester can disappear. The shape of the uterus and the alterations in the spacial relationship between placenta and internal os uteri were caused by the development and stretching of the lower segment of the uterus. The vaginal hemorrhages in the second trimester caused by a low placental insertion (13 cases) stopped (with the exception of 2 cases) at the latest after the 6th month of pregnancy. The definitive establishment of a placenta pravia before the 5th-6th month of pregnancy is virtually impossible sonographically.", "PMID": 976716}
{"id": "pubmed23n0032_10840", "title": "[Influence of lumbar peridural anaesthesia on fetal heart rate patterns in the second stage of labour (author's transl)].", "content": "The influence of lumbar peridural anaesthesia (PA) on fetal heart rate patterns in the second stage of labour was studied in 218 vaginal deliveries without maternal and fetal risk. A CTG-Score as proposed by Hammacher was used to evaluate fetal heart rate patterns. No influence of PA on fetal heart rate in the second stage of labour was found with primiparae, whereas multiparae showed more normal patterns under PA than without PA. All patients were strictly kept in lateral position throughout the first and second stage of labour. The pushing-period in lateral position was limited to 30 minutes (pushing 3-times per 10 minutes).", "contents": "[Influence of lumbar peridural anaesthesia on fetal heart rate patterns in the second stage of labour (author's transl)]. The influence of lumbar peridural anaesthesia (PA) on fetal heart rate patterns in the second stage of labour was studied in 218 vaginal deliveries without maternal and fetal risk. A CTG-Score as proposed by Hammacher was used to evaluate fetal heart rate patterns. No influence of PA on fetal heart rate in the second stage of labour was found with primiparae, whereas multiparae showed more normal patterns under PA than without PA. All patients were strictly kept in lateral position throughout the first and second stage of labour. The pushing-period in lateral position was limited to 30 minutes (pushing 3-times per 10 minutes).", "PMID": 976717}
{"id": "pubmed23n0032_10841", "title": "[Physical and physiological principles of CO2 hysteroscopy (author's transl)].", "content": "Various possibilities of developing the uterine cavity for hysteroscopy were presented together with their advantages and disadvantages. The authors consider CO2 gas insufflation to be the method of choice. The physical, physiological and technical principles and data of the procedure were discussed. The construction and function of the Hysteroflators 1000 S, a special gas insufflation apparatus for hysteroscopy were also discussed.", "contents": "[Physical and physiological principles of CO2 hysteroscopy (author's transl)]. Various possibilities of developing the uterine cavity for hysteroscopy were presented together with their advantages and disadvantages. The authors consider CO2 gas insufflation to be the method of choice. The physical, physiological and technical principles and data of the procedure were discussed. The construction and function of the Hysteroflators 1000 S, a special gas insufflation apparatus for hysteroscopy were also discussed.", "PMID": 976718}
{"id": "pubmed23n0032_10842", "title": "[Patterns of sexual behavior. A study on gynecological patients (author's transl)].", "content": "With a standard questionnaire 7356 lying-in patients at the Department of Obstetrics and Gynecology, University Erlangen-N\u00fcrnberg, were asked about their social background and their sexual habits. First of all some statistical facts concerning the frequency of distribution: 20% of all women questioned had their first cohabitation before the age of seventeen. 53% had sexual intercourse with one man, 23% with two, the remainder with more than two men. Quite a number of social characteristics influenced the sexual behavior of women. Thus it was shown that women have their first cohabitation before the age of eighteen the sooner the lower their social status is and the more partners they had. Insufficient contraceptive precautions or the neglect of any contraception were mainly met with women of lower social status. The same correlation could be found between contraceptive behavior on the one hand and the parameters education and strong religious commitment on the other hand. In rural aereas contraceptive measures were used insufficiently or less often then in urban communities.", "contents": "[Patterns of sexual behavior. A study on gynecological patients (author's transl)]. With a standard questionnaire 7356 lying-in patients at the Department of Obstetrics and Gynecology, University Erlangen-N\u00fcrnberg, were asked about their social background and their sexual habits. First of all some statistical facts concerning the frequency of distribution: 20% of all women questioned had their first cohabitation before the age of seventeen. 53% had sexual intercourse with one man, 23% with two, the remainder with more than two men. Quite a number of social characteristics influenced the sexual behavior of women. Thus it was shown that women have their first cohabitation before the age of eighteen the sooner the lower their social status is and the more partners they had. Insufficient contraceptive precautions or the neglect of any contraception were mainly met with women of lower social status. The same correlation could be found between contraceptive behavior on the one hand and the parameters education and strong religious commitment on the other hand. In rural aereas contraceptive measures were used insufficiently or less often then in urban communities.", "PMID": 976719}
{"id": "pubmed23n0032_10843", "title": "[Studies on the birth-weight and the size of the new-born child with reference to the parity of the mother (author's transl)].", "content": "The relation between the parity of the mother and the birth-weight as well as the size of the new-born child, taking into account the sex, were examined as follows: From the patients needing obstretical care during the period of 1966 to 1971 those 3441 women were chosen who had born twice or more times during this time. By means of an electronic data processing system the weight and size differences of the children were correlated with the parity of the mother taking again into account the order of male and female births. Births with uncertain gestational age, non-cephalic presentations, and premature births were excluded (WHO definition or gestational age less than or equal to 266 days counted from last menstruation). 1. The average birth-weight increases by 105 g from the first to the second child, by 39 g from the second to the third, and by 12 g from the third to the fourth child. Obviously, there is no linear link between the increase of the birth-weight and the increasing parity. The major weight-increase is to be observed from the first to the second child. 2. A corresponding statement concerning the size of the new-born children cannot be made which is partly due to the relative inaccuracy of the method applied. 3. On the average boys weigh between 130 g to 150 g more than girls according to the parity.", "contents": "[Studies on the birth-weight and the size of the new-born child with reference to the parity of the mother (author's transl)]. The relation between the parity of the mother and the birth-weight as well as the size of the new-born child, taking into account the sex, were examined as follows: From the patients needing obstretical care during the period of 1966 to 1971 those 3441 women were chosen who had born twice or more times during this time. By means of an electronic data processing system the weight and size differences of the children were correlated with the parity of the mother taking again into account the order of male and female births. Births with uncertain gestational age, non-cephalic presentations, and premature births were excluded (WHO definition or gestational age less than or equal to 266 days counted from last menstruation). 1. The average birth-weight increases by 105 g from the first to the second child, by 39 g from the second to the third, and by 12 g from the third to the fourth child. Obviously, there is no linear link between the increase of the birth-weight and the increasing parity. The major weight-increase is to be observed from the first to the second child. 2. A corresponding statement concerning the size of the new-born children cannot be made which is partly due to the relative inaccuracy of the method applied. 3. On the average boys weigh between 130 g to 150 g more than girls according to the parity.", "PMID": 976720}
{"id": "pubmed23n0032_10844", "title": "[A study about 3rd degree perinatal rupture (author's transl)].", "content": "29 cases of 3rd degree perineal rupture occured from 1962 to 1975 at the St\u00e4dtische Frauenklinik and Hebammenlehranstalt Braunschweig and have been analysed retrospectively. Most of these were observed in the last five years. The increase is undoubtlessly related to the shorter expulsion peried by ocytocin infusion. Investigations for avoidable courses and how to reduce them to a minimum were made in this analysis. Not at least this study was motivated by the lack of informations about obstetrical complications and the eventual consequences in literature. The only prophylaxis is the adequate support of the perineum and the sufficient episiotomy at the right moment. According to the operative and post operative treatment mentioned there could be achieved a good functional result in all cases. The stationary treatment has been reduced by these measures from 19,0 to 10,5 days.", "contents": "[A study about 3rd degree perinatal rupture (author's transl)]. 29 cases of 3rd degree perineal rupture occured from 1962 to 1975 at the St\u00e4dtische Frauenklinik and Hebammenlehranstalt Braunschweig and have been analysed retrospectively. Most of these were observed in the last five years. The increase is undoubtlessly related to the shorter expulsion peried by ocytocin infusion. Investigations for avoidable courses and how to reduce them to a minimum were made in this analysis. Not at least this study was motivated by the lack of informations about obstetrical complications and the eventual consequences in literature. The only prophylaxis is the adequate support of the perineum and the sufficient episiotomy at the right moment. According to the operative and post operative treatment mentioned there could be achieved a good functional result in all cases. The stationary treatment has been reduced by these measures from 19,0 to 10,5 days.", "PMID": 976721}
{"id": "pubmed23n0032_10845", "title": "[The problem of Caesarean section (author's transl)].", "content": "The Caesarean sections at the Universit\u00e4ts-Frauenklinik Innsbruck from January 1st, 1969 to December 31st, 1974 have been surveyed. Related to the total number of 12 579 deliveries 627 Caesarean sections have been performed. Two 3-year-intervals have been compared: 1969-1971 (group I) without and 1972-1974 (group II) with modern practices of prenatal supervision. There was a high significant increase of the section rate in group II (5,8% versus 4,1% which has been related to the increasing number of mainly fetal indications. The maternal morbidity didn't rise with the increasing section rate. A significant decrease in total infant mortality rate and mortality rate at vaginal deliveries has been found with the increasing incidence of Caesarean sections; whereas the number of depressed infants (Apgar score less than 7) was increased in group II. The reasons therefore have been discussed. The rise of the number of Caesarean sections in group II performed because of breech presentation (14,2% versus 6,2%) was correspondent with a statistically significant decrease in the mortality rate from 11,7 to 4,1%. A Caesarean section rate between 7% and 10% seems to be the limit at least in our hospital--the total infant mortality rate cannot be reduced by further increasing the section frequency: the infant mortality rate possibly could be lowered by a better and more wide spread prenatal care especially by taking more care for prematurity and by intensifying the perinatal period.", "contents": "[The problem of Caesarean section (author's transl)]. The Caesarean sections at the Universit\u00e4ts-Frauenklinik Innsbruck from January 1st, 1969 to December 31st, 1974 have been surveyed. Related to the total number of 12 579 deliveries 627 Caesarean sections have been performed. Two 3-year-intervals have been compared: 1969-1971 (group I) without and 1972-1974 (group II) with modern practices of prenatal supervision. There was a high significant increase of the section rate in group II (5,8% versus 4,1% which has been related to the increasing number of mainly fetal indications. The maternal morbidity didn't rise with the increasing section rate. A significant decrease in total infant mortality rate and mortality rate at vaginal deliveries has been found with the increasing incidence of Caesarean sections; whereas the number of depressed infants (Apgar score less than 7) was increased in group II. The reasons therefore have been discussed. The rise of the number of Caesarean sections in group II performed because of breech presentation (14,2% versus 6,2%) was correspondent with a statistically significant decrease in the mortality rate from 11,7 to 4,1%. A Caesarean section rate between 7% and 10% seems to be the limit at least in our hospital--the total infant mortality rate cannot be reduced by further increasing the section frequency: the infant mortality rate possibly could be lowered by a better and more wide spread prenatal care especially by taking more care for prematurity and by intensifying the perinatal period.", "PMID": 976722}
{"id": "pubmed23n0032_10846", "title": "[Comparison of labour induction with intravenous prostaglandin E2 and intravenous oxytocin (author's transl)].", "content": "Based on our results we are able to attribute to the PGE2 a special importance during the beginning of cervical dilatation. A cervical dilatation up to 3 cm is faster achieved by cervical relaxation effect and a direct effect on myometrial tissue independant from exterior parameters. The earlier the cervix has reached such a dilatation, the earlier regular contractions are registered at inductions with PGE2. These contractions are furthermore more effective and led to a shorter induction delivery interval under a minimal physiologic stress. This procedure is combined to a lower intrauterine pressure and shows no negative sideeffects on child and mother.", "contents": "[Comparison of labour induction with intravenous prostaglandin E2 and intravenous oxytocin (author's transl)]. Based on our results we are able to attribute to the PGE2 a special importance during the beginning of cervical dilatation. A cervical dilatation up to 3 cm is faster achieved by cervical relaxation effect and a direct effect on myometrial tissue independant from exterior parameters. The earlier the cervix has reached such a dilatation, the earlier regular contractions are registered at inductions with PGE2. These contractions are furthermore more effective and led to a shorter induction delivery interval under a minimal physiologic stress. This procedure is combined to a lower intrauterine pressure and shows no negative sideeffects on child and mother.", "PMID": 976723}
{"id": "pubmed23n0032_10847", "title": "[Investigations on the occurrence of mycoplasma in the amniotic fluid (author's transl)].", "content": "In order to elucidate the pathogenetic importance of mycoplasma in amnionitis and in infectious syndrome of the amnion, the amniotic fluid of 185 healthy pregnant women was investigated for mycoplasma, bacteria and fungi. In no case mycoplasma were detected. This means that amniotic fluid is normally free of mycoplasma. If mycoplasma occurs in the amniotic fluid it has to be considered as a possible cause of amnionitis and infectious syndrome of the amnion.", "contents": "[Investigations on the occurrence of mycoplasma in the amniotic fluid (author's transl)]. In order to elucidate the pathogenetic importance of mycoplasma in amnionitis and in infectious syndrome of the amnion, the amniotic fluid of 185 healthy pregnant women was investigated for mycoplasma, bacteria and fungi. In no case mycoplasma were detected. This means that amniotic fluid is normally free of mycoplasma. If mycoplasma occurs in the amniotic fluid it has to be considered as a possible cause of amnionitis and infectious syndrome of the amnion.", "PMID": 976724}
{"id": "pubmed23n0032_10848", "title": "[Seasonal variations in the birth rate and conception rate within the last 200 years (author's transl)].", "content": "The monthly variations and the mean daily conception rates of 136,828 deliveries from 1803 to 1970 within the same population area are reviewed. It was found that the conception rate during the first half of the 19th century showed a rapid increase from its low during the winter to its maximum in May and that this pattern became much less pronounced in the 20th century. During the second half of the 20th century, the highest conception rate shifted for the first time to the summer and the third quarter of the year. In contradistinction to this development, the low of the conception rate during the winter from January to March is much less pronounced during the first half of the 20th Century and remains constant with the lowest conception rate in February into the second half of the 20th century. The importance of the influence of nature during the spring and winter and of the working and living patterns as well as the influence of family planning and recreational activities on the intensity of procreation in the human within the last 200 years is discussed.", "contents": "[Seasonal variations in the birth rate and conception rate within the last 200 years (author's transl)]. The monthly variations and the mean daily conception rates of 136,828 deliveries from 1803 to 1970 within the same population area are reviewed. It was found that the conception rate during the first half of the 19th century showed a rapid increase from its low during the winter to its maximum in May and that this pattern became much less pronounced in the 20th century. During the second half of the 20th century, the highest conception rate shifted for the first time to the summer and the third quarter of the year. In contradistinction to this development, the low of the conception rate during the winter from January to March is much less pronounced during the first half of the 20th Century and remains constant with the lowest conception rate in February into the second half of the 20th century. The importance of the influence of nature during the spring and winter and of the working and living patterns as well as the influence of family planning and recreational activities on the intensity of procreation in the human within the last 200 years is discussed.", "PMID": 976725}
{"id": "pubmed23n0032_10849", "title": "[A modification of the bladder flap of Boari for the management of defects of the distal ureter (author's transl)].", "content": "The results of the management of defects of the distal ureter of various causes with a bladder flap of Boari including the anti-reflux implantation of the ureter in 65 patients are reported. 50 patients had prior gynaecological operations. The other 15 patients had prior urological operation, inflammations (tuberculosis), tumors or anomalies of the ureter. The follow-up time ranges from a few weeks to five years and 80-90% of the patients were cured. 24 of these patients had prior malignant tumors.", "contents": "[A modification of the bladder flap of Boari for the management of defects of the distal ureter (author's transl)]. The results of the management of defects of the distal ureter of various causes with a bladder flap of Boari including the anti-reflux implantation of the ureter in 65 patients are reported. 50 patients had prior gynaecological operations. The other 15 patients had prior urological operation, inflammations (tuberculosis), tumors or anomalies of the ureter. The follow-up time ranges from a few weeks to five years and 80-90% of the patients were cured. 24 of these patients had prior malignant tumors.", "PMID": 976726}
{"id": "pubmed23n0032_10850", "title": "H1- and H2-receptors for histamine in the ileum of the guinea pig.", "content": "Clark's equation used in its inverted form (double reciprocal plots) was shown to be valid regarding the interaction of histamine with its receptors in the guinea-pig ileum. Burimamide, a typical H2-receptor antagonist potentiated the stimulating effect of small concentrations of histamine (10(-6) M) on the intestinal smooth muscle, probably, by blocking H2-receptors which may show the opposite effect. The calculated parameters of affinity (pKn) remained constant under different conditions. A linear correlation between length of the preparation and maximal contractions has been found.", "contents": "H1- and H2-receptors for histamine in the ileum of the guinea pig. Clark's equation used in its inverted form (double reciprocal plots) was shown to be valid regarding the interaction of histamine with its receptors in the guinea-pig ileum. Burimamide, a typical H2-receptor antagonist potentiated the stimulating effect of small concentrations of histamine (10(-6) M) on the intestinal smooth muscle, probably, by blocking H2-receptors which may show the opposite effect. The calculated parameters of affinity (pKn) remained constant under different conditions. A linear correlation between length of the preparation and maximal contractions has been found.", "PMID": 976728}
{"id": "pubmed23n0032_10851", "title": "Supersensitivity induced by the toxin of Brazilian scorpion venom (Tityus serrulatus Lutz e Mello).", "content": "The purified toxin of the Brazilian scorpion venom (TsTX) enhances the pharmacological actions of directly and indirectly acting substances on several preparations. The enhancing action of TsTX is more pronounced for the indirectly acting drugs, which is highly suggestive of a prejunctional site of action. Secondarily, TsTX does also produce an unspecific increase of sensitivity at the level of the pharmacological receptor itself. The mechanism by which these changes are brought about remains to be determined.", "contents": "Supersensitivity induced by the toxin of Brazilian scorpion venom (Tityus serrulatus Lutz e Mello). The purified toxin of the Brazilian scorpion venom (TsTX) enhances the pharmacological actions of directly and indirectly acting substances on several preparations. The enhancing action of TsTX is more pronounced for the indirectly acting drugs, which is highly suggestive of a prejunctional site of action. Secondarily, TsTX does also produce an unspecific increase of sensitivity at the level of the pharmacological receptor itself. The mechanism by which these changes are brought about remains to be determined.", "PMID": 976729}
{"id": "pubmed23n0032_10852", "title": "Cholinergic and adrenergic effects of tityustoxin.", "content": "The effects of purified scorpion toxin (Tityustoxin or TsTX) were investigated on the isolated guinea-pig and rat ileum, rat spleen strip and hen rectal caecum. The contraction of the ileum was due only in part to the release of acetylcholine, whereas the contraction of the spleen strip and the relaxation of the rectal caecum were due to the release of catecholamines.", "contents": "Cholinergic and adrenergic effects of tityustoxin. The effects of purified scorpion toxin (Tityustoxin or TsTX) were investigated on the isolated guinea-pig and rat ileum, rat spleen strip and hen rectal caecum. The contraction of the ileum was due only in part to the release of acetylcholine, whereas the contraction of the spleen strip and the relaxation of the rectal caecum were due to the release of catecholamines.", "PMID": 976730}
{"id": "pubmed23n0032_10853", "title": "Potentiation of bradykinin action on smooth muscle by a scorpion venom extract.", "content": "Gel filtration of the water extract of the venom of the scorpion T. serrulatus showed four peaks; the first peak (P1) is devoid of toxic activity but increases the bradykinin-induced contraction of isolated rat uterus and guinea-pig ileum. The stepwise fractionation of the pooled P1 peak was performed in a DEAE-cellulose column and the bradykinin potentiating activity was found in the second protein peak. Finger-printing of this material showed that the bradykinin potentiating material migrates to the anode, giving two spots when submitted to chromatography, the activity being found in the spot that presents the greatest Rf. The potentiator is destroyed by heating at 97 degrees C, is not dialysable and is destroyed by incubation with pronase. Some of these properties differentiate it from the BPF's from snake venoms.", "contents": "Potentiation of bradykinin action on smooth muscle by a scorpion venom extract. Gel filtration of the water extract of the venom of the scorpion T. serrulatus showed four peaks; the first peak (P1) is devoid of toxic activity but increases the bradykinin-induced contraction of isolated rat uterus and guinea-pig ileum. The stepwise fractionation of the pooled P1 peak was performed in a DEAE-cellulose column and the bradykinin potentiating activity was found in the second protein peak. Finger-printing of this material showed that the bradykinin potentiating material migrates to the anode, giving two spots when submitted to chromatography, the activity being found in the spot that presents the greatest Rf. The potentiator is destroyed by heating at 97 degrees C, is not dialysable and is destroyed by incubation with pronase. Some of these properties differentiate it from the BPF's from snake venoms.", "PMID": 976731}
{"id": "pubmed23n0032_10854", "title": "Calcium and the muscarinic receptor.", "content": "Pharmacological receptors may be viewed as composed of two linked functions, a recognition site through which the specificity and selectivity of ligand action is expressed and an amplification or catalytic site which translates the ligand-recognition site interaction into response. The successful elucidation of receptor mechanisms requires analysis of both sites. The muscarinic cholinergic receptors of guinea-pig ileal longitudinal smooth muscle will be discussed from this two function stand point and the source and utilization of Ca2+ in excitation-contraction coupling discussed.", "contents": "Calcium and the muscarinic receptor. Pharmacological receptors may be viewed as composed of two linked functions, a recognition site through which the specificity and selectivity of ligand action is expressed and an amplification or catalytic site which translates the ligand-recognition site interaction into response. The successful elucidation of receptor mechanisms requires analysis of both sites. The muscarinic cholinergic receptors of guinea-pig ileal longitudinal smooth muscle will be discussed from this two function stand point and the source and utilization of Ca2+ in excitation-contraction coupling discussed.", "PMID": 976732}
{"id": "pubmed23n0032_10855", "title": "Origins of the spontaneous receptor potential in the tuberous organ in a mormyrid fish.", "content": "In an electroreceptor of a Mormyrid fish: 1. The correlations between the number of sensory cells in each receptor organ and the mode of the intervals of time between receptor potentials are not significant within the temperature ranges of 20.0-26.0 degrees C, 20.5-21.5 degrees C, 22.0-23.0 degrees C and are significant at a level of 4.9% within 25.0-26.0 degrees C. 2. The correlations between the mode of intervals of time between receptor potentials and temperature are significant at a level inferior to 1% for isolated receptor organs and also at a level of 5.8% for 21 different receptor organs from 9 different fishes.", "contents": "Origins of the spontaneous receptor potential in the tuberous organ in a mormyrid fish. In an electroreceptor of a Mormyrid fish: 1. The correlations between the number of sensory cells in each receptor organ and the mode of the intervals of time between receptor potentials are not significant within the temperature ranges of 20.0-26.0 degrees C, 20.5-21.5 degrees C, 22.0-23.0 degrees C and are significant at a level of 4.9% within 25.0-26.0 degrees C. 2. The correlations between the mode of intervals of time between receptor potentials and temperature are significant at a level inferior to 1% for isolated receptor organs and also at a level of 5.8% for 21 different receptor organs from 9 different fishes.", "PMID": 976733}
{"id": "pubmed23n0032_10856", "title": "The role of central muscarinic and nicotinic receptors in the regulation of sodium and potassium renal excretion.", "content": "The effect of intraseptal injection of carbachol and nicotine on urinary output of Na+ and K+ in untreated rats as well as in animals pretreated with locally injected atropine, hexamethonium, dibenamine and propranolol was studied in order to evaluate the relative role played by central muscarinic and nicotinic receptors in the regulation of salt and water renal excretion. The injection of 30-250 nmol of nicotine into the medial septal area caused a dose-dependent increase in Na+ and K+ urinary output whereas urine volume was little affected. The effect of 30 nmol of nicotine was blocked by pretreatment with 100 nmol of hexamethonium. In addition, pretreatment with 5 nmol of either hexamethonium or atropine partially antagonized the natriuretic and kaliuretic effect of 1 nmol of carbachol. Also the alpha-blocking agent, dibenamine (150 nmol) antagonized, while the beta-blocker, propranolol (100 nmol) significantly enhanced the effect of carbachol. Propranolol (100 nmol) alone caused a small, but significant increase in Na+ and K+ renal excretion. These results indicate that stimulation of both muscarinic and nicotinic receptors in the septal area, as caused by carbachol, elicits increased disposition of Na+ and K+ by the kidneys. Also, part of the effects of carbachol appear to be mediated by the release of endogenous catecholamines, acting on central alpha receptors to increase Na+ and K+ urinary excretion. On the other hand, simultaneous activation of beta-receptors by the released amines would partially inhibit this effect.", "contents": "The role of central muscarinic and nicotinic receptors in the regulation of sodium and potassium renal excretion. The effect of intraseptal injection of carbachol and nicotine on urinary output of Na+ and K+ in untreated rats as well as in animals pretreated with locally injected atropine, hexamethonium, dibenamine and propranolol was studied in order to evaluate the relative role played by central muscarinic and nicotinic receptors in the regulation of salt and water renal excretion. The injection of 30-250 nmol of nicotine into the medial septal area caused a dose-dependent increase in Na+ and K+ urinary output whereas urine volume was little affected. The effect of 30 nmol of nicotine was blocked by pretreatment with 100 nmol of hexamethonium. In addition, pretreatment with 5 nmol of either hexamethonium or atropine partially antagonized the natriuretic and kaliuretic effect of 1 nmol of carbachol. Also the alpha-blocking agent, dibenamine (150 nmol) antagonized, while the beta-blocker, propranolol (100 nmol) significantly enhanced the effect of carbachol. Propranolol (100 nmol) alone caused a small, but significant increase in Na+ and K+ renal excretion. These results indicate that stimulation of both muscarinic and nicotinic receptors in the septal area, as caused by carbachol, elicits increased disposition of Na+ and K+ by the kidneys. Also, part of the effects of carbachol appear to be mediated by the release of endogenous catecholamines, acting on central alpha receptors to increase Na+ and K+ urinary excretion. On the other hand, simultaneous activation of beta-receptors by the released amines would partially inhibit this effect.", "PMID": 976734}
{"id": "pubmed23n0032_10857", "title": "Guanidines as analogs of histamine and antihistamines on the isolated guinea-pig ileum.", "content": "The present work describes the interaction of phenylguanidine and cyclohexylguanidine with receptor structures of the isolated guinea-pig ileum. It is suggested that phenylguanidine in the range of 0.2-2 mM, acts in a way similar to histamine. At lower concentrations in the bath, phenylguanidine and cyclohexylguanidine, behave as competitive antagonists of histamine, with Ki values close to 0.1 mM and 0.4 mM, respectively. At higher concentrations phenylguanidine acts as agonist with a Kn near 0.3 mM; the Kn for histamine was found to be 0.127 muM, in agreement with previous data from the literature. The agonistic action of phenylguanidine is inhibited by promethazine and atropine, which have Ki values of 0.342 muM and 0.575 muM, respectively. Cyclohexylguanidine is devoid of spasmogenic activity, and this was attributed to the lack of aromatic character and to the bulkiness of the cyclohexyl side chain.", "contents": "Guanidines as analogs of histamine and antihistamines on the isolated guinea-pig ileum. The present work describes the interaction of phenylguanidine and cyclohexylguanidine with receptor structures of the isolated guinea-pig ileum. It is suggested that phenylguanidine in the range of 0.2-2 mM, acts in a way similar to histamine. At lower concentrations in the bath, phenylguanidine and cyclohexylguanidine, behave as competitive antagonists of histamine, with Ki values close to 0.1 mM and 0.4 mM, respectively. At higher concentrations phenylguanidine acts as agonist with a Kn near 0.3 mM; the Kn for histamine was found to be 0.127 muM, in agreement with previous data from the literature. The agonistic action of phenylguanidine is inhibited by promethazine and atropine, which have Ki values of 0.342 muM and 0.575 muM, respectively. Cyclohexylguanidine is devoid of spasmogenic activity, and this was attributed to the lack of aromatic character and to the bulkiness of the cyclohexyl side chain.", "PMID": 976735}
{"id": "pubmed23n0032_10858", "title": "Rabbit brain thiol-activated endopeptidase. Hydrolysis of bradykinin and kininogen.", "content": "A neutral brain endopeptidase which hydrolyzes bradykinin (Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) at the Phe5-Ser6 peptide bond was activated about 10 times by dithiothreitol. The preferential specificity of the enzyme for small peptides was suggested on the basis of the absence of activity toward a bradykinin-related protein such as S-carboxymethylated plasma kininogen.", "contents": "Rabbit brain thiol-activated endopeptidase. Hydrolysis of bradykinin and kininogen. A neutral brain endopeptidase which hydrolyzes bradykinin (Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) at the Phe5-Ser6 peptide bond was activated about 10 times by dithiothreitol. The preferential specificity of the enzyme for small peptides was suggested on the basis of the absence of activity toward a bradykinin-related protein such as S-carboxymethylated plasma kininogen.", "PMID": 976736}
{"id": "pubmed23n0032_10859", "title": "Localization of acetylcholinesterase in a crustacean neuromuscular system.", "content": "1. The localization of acetylcholinesterase (AChE) was studied by light and electron microscopy (LM and EM) at the neuromuscular junctions (NMJs) of the abdominal superficial flexor muscle (SFM) and the claw adductor muscle (CAM) of the crayfish in view of reported pharmacological evidence of cholinergic transmission at the former but not at the latter site. 2. Survey of LM with the copper-thiocholine method disclosed staining for AChE, but not for butyrocholinesterase (BuChE), at numerous NMJs of the SFM; neither enzyme was detected at any NMJs of the CAM. 3. With the bis-(thioacetoxy) aurate (I) method, EM examination showed AChE-staining at NMJs of the SFM to be confined to the postjunctional membrane. 4. The published and present findings suggest a unique situation of more than one excitatory transmitter for the skeletal neuromuscular system in the crustacean studied.", "contents": "Localization of acetylcholinesterase in a crustacean neuromuscular system. 1. The localization of acetylcholinesterase (AChE) was studied by light and electron microscopy (LM and EM) at the neuromuscular junctions (NMJs) of the abdominal superficial flexor muscle (SFM) and the claw adductor muscle (CAM) of the crayfish in view of reported pharmacological evidence of cholinergic transmission at the former but not at the latter site. 2. Survey of LM with the copper-thiocholine method disclosed staining for AChE, but not for butyrocholinesterase (BuChE), at numerous NMJs of the SFM; neither enzyme was detected at any NMJs of the CAM. 3. With the bis-(thioacetoxy) aurate (I) method, EM examination showed AChE-staining at NMJs of the SFM to be confined to the postjunctional membrane. 4. The published and present findings suggest a unique situation of more than one excitatory transmitter for the skeletal neuromuscular system in the crustacean studied.", "PMID": 976738}
{"id": "pubmed23n0032_10860", "title": "Role of spleen in the interferon-lowering action of morphine.", "content": "1. Polyinosinie-polycytidylic acid (Poly I:C) administered intraperitioneally to mice produced an increase in the level of interferon in the tissues of the spleen, liver, lung, and kidney. The level of circulating serum interferon also was increased by the polynucleotide. 2. The highest titer of interferon, presumably resulting from the greatest response to poly I:C was measured in the splenic tissue. 3. Morphine inhibited the poly I:C induced increase in all tissues and circulating serum. The decrease in interferon titer was similar in spleen and serum. 4. These results may indicate the serum level of interferon was more closely related and dependent upon events occuring in the spleen than in the other tissues.", "contents": "Role of spleen in the interferon-lowering action of morphine. 1. Polyinosinie-polycytidylic acid (Poly I:C) administered intraperitioneally to mice produced an increase in the level of interferon in the tissues of the spleen, liver, lung, and kidney. The level of circulating serum interferon also was increased by the polynucleotide. 2. The highest titer of interferon, presumably resulting from the greatest response to poly I:C was measured in the splenic tissue. 3. Morphine inhibited the poly I:C induced increase in all tissues and circulating serum. The decrease in interferon titer was similar in spleen and serum. 4. These results may indicate the serum level of interferon was more closely related and dependent upon events occuring in the spleen than in the other tissues.", "PMID": 976739}
{"id": "pubmed23n0032_10861", "title": "Centrally-acting drugs and the formation of brain gamma-aminobutyric acid.", "content": "1. The effects of a number of centrally-acting compoinds were tested on the levels of GABA and glutamate and on the formation of GABA from glutamate in rat brain. 2. Pargyline and desipramine increased the brain concentration of GABA but were without effect on GABA production. 3. Imidazoleacetic acid increased GABA levels, decreased glutamate levels but did not alter GABA production, whereas gamma-hydroxybutyric acid had an effect only on GABA formation. 4. Isonicotinc acid hydrazine caused a marked decrease in the formation of GABA from glutamate. 5. Chlorpromazine, diphenylhydantoin, bicucculine, and L-DOPA had no effect on any of the biochemical parameters measured. 6. The central effects of these drugs are discussed in relation to the above findings.", "contents": "Centrally-acting drugs and the formation of brain gamma-aminobutyric acid. 1. The effects of a number of centrally-acting compoinds were tested on the levels of GABA and glutamate and on the formation of GABA from glutamate in rat brain. 2. Pargyline and desipramine increased the brain concentration of GABA but were without effect on GABA production. 3. Imidazoleacetic acid increased GABA levels, decreased glutamate levels but did not alter GABA production, whereas gamma-hydroxybutyric acid had an effect only on GABA formation. 4. Isonicotinc acid hydrazine caused a marked decrease in the formation of GABA from glutamate. 5. Chlorpromazine, diphenylhydantoin, bicucculine, and L-DOPA had no effect on any of the biochemical parameters measured. 6. The central effects of these drugs are discussed in relation to the above findings.", "PMID": 976740}
{"id": "pubmed23n0032_10862", "title": "Hepatic and duodenal drug metabolism in the rat during fat deficiency.", "content": "1. The effect of dietary fat deficiency on the composition of hepatic microsomes and postmitochondrial supernatant fraction of duodenal mucosa and on the activities of drug-metabolizing enzymes was studied in the rat. 2. There were only little changes in the measured protein, cholesterol and phospholipid fractions in the liver microsomes. In the postmitochondrial supernatant fraction of the duodenal mucosa, however, protein content was increased during fat deficiency. 3. In the fatty acid composition of hepatic microsomal phospholipids marked differences took place during fat deficiency. The amount of palmitoleic and eicosatrienoic acids increased from undetectable level to the amount of 6.9 and 9.5%. Also the amount of oleic acid was almost doubled and the content of linoleic and arachidonic acid was markedly decreased. 4. Fat deficiency was found to affect the drug metabolism both in the liver and small intestine. Only minor changes took place in the measured hydroxylative enzyme activities but the activity of UDPglucuronosyltransferase was highly depressed during fat deficiency both in the liver microsomes and in the postmitochondrial supernatant of duodenal mucosa. The changes in the UDPglucuronosyltransferase activity are concluded to be mediated via changes in the lipoidal environment of the enzyme in the membranes.", "contents": "Hepatic and duodenal drug metabolism in the rat during fat deficiency. 1. The effect of dietary fat deficiency on the composition of hepatic microsomes and postmitochondrial supernatant fraction of duodenal mucosa and on the activities of drug-metabolizing enzymes was studied in the rat. 2. There were only little changes in the measured protein, cholesterol and phospholipid fractions in the liver microsomes. In the postmitochondrial supernatant fraction of the duodenal mucosa, however, protein content was increased during fat deficiency. 3. In the fatty acid composition of hepatic microsomal phospholipids marked differences took place during fat deficiency. The amount of palmitoleic and eicosatrienoic acids increased from undetectable level to the amount of 6.9 and 9.5%. Also the amount of oleic acid was almost doubled and the content of linoleic and arachidonic acid was markedly decreased. 4. Fat deficiency was found to affect the drug metabolism both in the liver and small intestine. Only minor changes took place in the measured hydroxylative enzyme activities but the activity of UDPglucuronosyltransferase was highly depressed during fat deficiency both in the liver microsomes and in the postmitochondrial supernatant of duodenal mucosa. The changes in the UDPglucuronosyltransferase activity are concluded to be mediated via changes in the lipoidal environment of the enzyme in the membranes.", "PMID": 976741}
{"id": "pubmed23n0032_10863", "title": "The effect of oxygen on the enzymes concerned in the metabolism of 5-hydroxytryptamine by rat brain and liver preparations.", "content": "1. The effect of oxygen concentration at 1 atm on rat enzyme systems concerned in the synthesis and degradation of 5-hydroxytryptamine was studied in vitro. 2. Oxygen activated tryptophan hydroxylase and the deamination of both 5-hydroxytryptamine and tyramine by monoamine oxidase. 3. Oxygen did not affect the activities of 5-hydroxytryptophan decarboxylase or peroxidase.", "contents": "The effect of oxygen on the enzymes concerned in the metabolism of 5-hydroxytryptamine by rat brain and liver preparations. 1. The effect of oxygen concentration at 1 atm on rat enzyme systems concerned in the synthesis and degradation of 5-hydroxytryptamine was studied in vitro. 2. Oxygen activated tryptophan hydroxylase and the deamination of both 5-hydroxytryptamine and tyramine by monoamine oxidase. 3. Oxygen did not affect the activities of 5-hydroxytryptophan decarboxylase or peroxidase.", "PMID": 976742}
{"id": "pubmed23n0032_10864", "title": "In vitro metabolism of aniline by sheep intestine.", "content": "1. The major metabolite after incubating aniline with sheep intestine was acetanilide. 2. Other metabolites detected in smaller amounts were 2-aminophenol, 4-aminophenol, 2-acetamidophenol and 4-acetamidophenol. 3. The rumen, abomasum duodenum, jejunum, ileum and colon were all able to acetylate aniline. 4. 4-Aminophenol, 4-aminobenzoic acid, 4-anisidine and 4-nitroaniline were also acetylated.", "contents": "In vitro metabolism of aniline by sheep intestine. 1. The major metabolite after incubating aniline with sheep intestine was acetanilide. 2. Other metabolites detected in smaller amounts were 2-aminophenol, 4-aminophenol, 2-acetamidophenol and 4-acetamidophenol. 3. The rumen, abomasum duodenum, jejunum, ileum and colon were all able to acetylate aniline. 4. 4-Aminophenol, 4-aminobenzoic acid, 4-anisidine and 4-nitroaniline were also acetylated.", "PMID": 976743}
{"id": "pubmed23n0032_10865", "title": "A comparison of the effect of propylthiouracil on the respiration of thyroid slices from humans, chickens and rats.", "content": "1. The QO2 of thyroid slices from hyperthyroid patients treated with propylthiouracil (PTU) was 140% that of slices from euthyroid patients. Similar results were obtained using thyroid slices from PTU fed and control chickens. 2. PTU feeding caused thyroid hyperplasia, increased QO2 more than could be accounted for by the hyperplasia but neither increased QO2 of muscle, cerebral cortex or spleen nor altered serum T4 levels. 3. PTU in drinking water increased the size and vascularity of thyroids from control but not hypophysectomized rats and had no effect on QO2. 4. The observations made on human, chicken and rat thryoid apparently reflect the effects of TSH rather than a direct action of PTU.", "contents": "A comparison of the effect of propylthiouracil on the respiration of thyroid slices from humans, chickens and rats. 1. The QO2 of thyroid slices from hyperthyroid patients treated with propylthiouracil (PTU) was 140% that of slices from euthyroid patients. Similar results were obtained using thyroid slices from PTU fed and control chickens. 2. PTU feeding caused thyroid hyperplasia, increased QO2 more than could be accounted for by the hyperplasia but neither increased QO2 of muscle, cerebral cortex or spleen nor altered serum T4 levels. 3. PTU in drinking water increased the size and vascularity of thyroids from control but not hypophysectomized rats and had no effect on QO2. 4. The observations made on human, chicken and rat thryoid apparently reflect the effects of TSH rather than a direct action of PTU.", "PMID": 976744}
{"id": "pubmed23n0032_10866", "title": "Body build and behavior in emotionally disturbed Dutch children.", "content": "Some physique-behavior relations were determined in 75 boys and 51 girls, aged 6 to 14, in a residential treatment center in Holland. Each child's behavior was rated by four group workers on a checklist yielding eight summary scores. Physique was judged from nude photographs for manifest endomorphy, mesomorphy, and ectomorphy. While a number of moderate-sized relations appeared in zero-order and multiple correlations, canonical analysis showed two significant, independent sets of relations between physique and behavior in the boys, one in the girls. Among the boys, one set related mesomorphy and energy level, the other related primarily ectomorphy with unsocialness, excitability, and cooperativeness. Among the girls, these same behaviors were compressed in a single set, primarily relating energy level but also unsocialness, excitability, and cooperativeness positively with mesomorphy and negatively with endomorphy and ectomorphy. Compared with findings on the same instrument in a sample of normal American preschoolers, many individual associations were similar, despite differences in culture and language, age, intelligence, social status, adjustment, and residential setting of the S's.", "contents": "Body build and behavior in emotionally disturbed Dutch children. Some physique-behavior relations were determined in 75 boys and 51 girls, aged 6 to 14, in a residential treatment center in Holland. Each child's behavior was rated by four group workers on a checklist yielding eight summary scores. Physique was judged from nude photographs for manifest endomorphy, mesomorphy, and ectomorphy. While a number of moderate-sized relations appeared in zero-order and multiple correlations, canonical analysis showed two significant, independent sets of relations between physique and behavior in the boys, one in the girls. Among the boys, one set related mesomorphy and energy level, the other related primarily ectomorphy with unsocialness, excitability, and cooperativeness. Among the girls, these same behaviors were compressed in a single set, primarily relating energy level but also unsocialness, excitability, and cooperativeness positively with mesomorphy and negatively with endomorphy and ectomorphy. Compared with findings on the same instrument in a sample of normal American preschoolers, many individual associations were similar, despite differences in culture and language, age, intelligence, social status, adjustment, and residential setting of the S's.", "PMID": 976749}
{"id": "pubmed23n0032_10867", "title": "[2 genetic systems of serum amylase in the Kholmogor breed of cattle].", "content": "All the genetic types of amylase I (AmI) and amylase II (AmII) known for the species Bos taurus are encountered in the Kholmogorskaya breed of cattle. Besides the two alleles, AmIB and AmIC found in all the other European breeds of cattle the locus in the Kholmogorskaya breed also includes a rare allele AmI-A hitherto considered to be characteristic only of zebu-like cattle. The frequencies of the alleles AmI-A, AmI-B and AmI-C in the Kholmogorskaya breed proved to be 0.014, 0.392 and 0.594 respectively (n = 1098). Of the two co-dominant alleles, AmII-A and AmII-B controlling the synthesis of the isozymes of the second amylase system the first is rare, occurring with the frequency of 0.131 (n = 245). The earlier assumption of Mazumder and Spooner, that there is no linkage between the genes of the loci AmI and AmII is corroborated. The breeds assigned to the Bos taurus brachyceros type have a lower frequencies of the AmI-C allele as compared to other breeds the origin of which is associated with the subspecies B. t. primigenius.", "contents": "[2 genetic systems of serum amylase in the Kholmogor breed of cattle]. All the genetic types of amylase I (AmI) and amylase II (AmII) known for the species Bos taurus are encountered in the Kholmogorskaya breed of cattle. Besides the two alleles, AmIB and AmIC found in all the other European breeds of cattle the locus in the Kholmogorskaya breed also includes a rare allele AmI-A hitherto considered to be characteristic only of zebu-like cattle. The frequencies of the alleles AmI-A, AmI-B and AmI-C in the Kholmogorskaya breed proved to be 0.014, 0.392 and 0.594 respectively (n = 1098). Of the two co-dominant alleles, AmII-A and AmII-B controlling the synthesis of the isozymes of the second amylase system the first is rare, occurring with the frequency of 0.131 (n = 245). The earlier assumption of Mazumder and Spooner, that there is no linkage between the genes of the loci AmI and AmII is corroborated. The breeds assigned to the Bos taurus brachyceros type have a lower frequencies of the AmI-C allele as compared to other breeds the origin of which is associated with the subspecies B. t. primigenius.", "PMID": 976750}
{"id": "pubmed23n0032_10868", "title": "Making sense of cerebral dominance and syndromes of the nondominant hemisphere.", "content": "Knowing the special function of each half of the brain is important to the clinician. If he doesn't understand what damage to the nondominant hemisphere can do, he may mistake the resulting syndrome for a psychiatric illness or generalized dementia, and the patient won't get proper treatment and counseling. The tendency is to think of dominance in terms of language, but other functions are asymmetrically located even though their input and output are bilateral. Generally, lesions in the left hemisphere cause problems with language and those in the right disrupt visual and spatial skills, attention, and emotional behavior. By far the most common syndrome in right hemisphere lesions is neglect of the left side. A patient may show lack of concern about his illness or he may deny that he is sick or even that the left side of his body belongs to him. Patients with left hemisphere lesions may neglect the right side but rarely to this extreme.", "contents": "Making sense of cerebral dominance and syndromes of the nondominant hemisphere. Knowing the special function of each half of the brain is important to the clinician. If he doesn't understand what damage to the nondominant hemisphere can do, he may mistake the resulting syndrome for a psychiatric illness or generalized dementia, and the patient won't get proper treatment and counseling. The tendency is to think of dominance in terms of language, but other functions are asymmetrically located even though their input and output are bilateral. Generally, lesions in the left hemisphere cause problems with language and those in the right disrupt visual and spatial skills, attention, and emotional behavior. By far the most common syndrome in right hemisphere lesions is neglect of the left side. A patient may show lack of concern about his illness or he may deny that he is sick or even that the left side of his body belongs to him. Patients with left hemisphere lesions may neglect the right side but rarely to this extreme.", "PMID": 976751}
{"id": "pubmed23n0032_10869", "title": "Significance of arrhythmias in acute respiratory failure.", "content": "Arrhythmias are as likely to strike patients with acute respiratory failure as patients with acute myocardial infarction. Both supraventricular and ventricular types were detected in half or more of the patients in two recent studies. Ventricular arrhythmias have a particularly bleak prognosis, since they often deteriorate into ventricular fibrillation or cardiac arrest. The exact causes of these arrhythmias are still a mystery, but metabolic abnormalities associated with respiratory failure are highly suspect. These disturbances can disrupt the transmembrane action potential of cardiac conducting tissue, causing electrophysiologic phenomena known to trigger arrhythmias. Until a specific etiology is confirmed, treatment should focus on identifying and correcting possible metabolic causes. Congestive heart failure--itself a cause of arrhythmias--also should be treated. Cardioversion and antiarrhythmic drugs should be used only in life-threatening situations.", "contents": "Significance of arrhythmias in acute respiratory failure. Arrhythmias are as likely to strike patients with acute respiratory failure as patients with acute myocardial infarction. Both supraventricular and ventricular types were detected in half or more of the patients in two recent studies. Ventricular arrhythmias have a particularly bleak prognosis, since they often deteriorate into ventricular fibrillation or cardiac arrest. The exact causes of these arrhythmias are still a mystery, but metabolic abnormalities associated with respiratory failure are highly suspect. These disturbances can disrupt the transmembrane action potential of cardiac conducting tissue, causing electrophysiologic phenomena known to trigger arrhythmias. Until a specific etiology is confirmed, treatment should focus on identifying and correcting possible metabolic causes. Congestive heart failure--itself a cause of arrhythmias--also should be treated. Cardioversion and antiarrhythmic drugs should be used only in life-threatening situations.", "PMID": 976756}
{"id": "pubmed23n0032_10870", "title": "Cor pulmonale: pathophysiology and management.", "content": "Pulmonary disease may set in motion a chain of events that ultimately leads to hypertrophy--or even failure--of the heart's right ventricle. The most common cause is chronic obstructive disease, which deprives the lungs of oxygen and produces pulmonary hypertension. But other disorders that raise pulmonary artery pressure also may be responsible. The thin right ventricle, which must work harder to overcome this increased resistance, ends up resembling the thick left ventricle. Comprehensive treatment of the primary lung condition at home usually enables the patient with chronic cor pulmonale to be more active and prevents frequent hospitalizations. Controlled-dose supplemental oxygen therapy is particularly effective, according to recent studies. Bronchospasm or bronchial infection super-imposed on the chronic lung condition may prove too much for the already strained right ventricle. Right ventricular failure calls for hospitalization and vigorous treatment, which may include mechanical ventilation, phlebotomy, antibiotics, steroids, digitalis, diuretics, and correction of electrolyte disturbances.", "contents": "Cor pulmonale: pathophysiology and management. Pulmonary disease may set in motion a chain of events that ultimately leads to hypertrophy--or even failure--of the heart's right ventricle. The most common cause is chronic obstructive disease, which deprives the lungs of oxygen and produces pulmonary hypertension. But other disorders that raise pulmonary artery pressure also may be responsible. The thin right ventricle, which must work harder to overcome this increased resistance, ends up resembling the thick left ventricle. Comprehensive treatment of the primary lung condition at home usually enables the patient with chronic cor pulmonale to be more active and prevents frequent hospitalizations. Controlled-dose supplemental oxygen therapy is particularly effective, according to recent studies. Bronchospasm or bronchial infection super-imposed on the chronic lung condition may prove too much for the already strained right ventricle. Right ventricular failure calls for hospitalization and vigorous treatment, which may include mechanical ventilation, phlebotomy, antibiotics, steroids, digitalis, diuretics, and correction of electrolyte disturbances.", "PMID": 976757}
{"id": "pubmed23n0032_10871", "title": "A possible mechanism for enhancement of increased production of tumor angiogenic factor.", "content": "It is suggested that anoxic conditions may enhance the transformation of tumor cells to increase production of tumor angiogenic factor. The theory of oxygen diffusion into a spherical mass is used to calculate an upper bound for the critical size at which anoxia begins to develop in the center of an avascular clone of tumor cells. The predicted size is in line with experimental observations of the maximum size of avascular tumors.", "contents": "A possible mechanism for enhancement of increased production of tumor angiogenic factor. It is suggested that anoxic conditions may enhance the transformation of tumor cells to increase production of tumor angiogenic factor. The theory of oxygen diffusion into a spherical mass is used to calculate an upper bound for the critical size at which anoxia begins to develop in the center of an avascular clone of tumor cells. The predicted size is in line with experimental observations of the maximum size of avascular tumors.", "PMID": 976765}
{"id": "pubmed23n0032_10872", "title": "Quantification of studies of blood flow in growing and irradiated tumors in C3H mice.", "content": "An approach to quantification of blood flow in growing malignant tumors, by relating the specific rate of change of flow to the specific rate of change of tumor weight or volume, was discussed. This model, previously noted to hold for many animal tumors, offers a good description of blood flow in the KHT sarcoma in C3H mice. The same tumor responds to radiation therapy, and simple expressions were given of the relationship of flow per unit volume, at 2 points in time, to the original radiation dose. Since most animal tumors show a nonviable or necrotic volume, a description of the phenomenon was given for the C3H/Bi mammary carcinoma by noting that the percent of nonviable tissue approaches a limiting (asymptomic)value.", "contents": "Quantification of studies of blood flow in growing and irradiated tumors in C3H mice. An approach to quantification of blood flow in growing malignant tumors, by relating the specific rate of change of flow to the specific rate of change of tumor weight or volume, was discussed. This model, previously noted to hold for many animal tumors, offers a good description of blood flow in the KHT sarcoma in C3H mice. The same tumor responds to radiation therapy, and simple expressions were given of the relationship of flow per unit volume, at 2 points in time, to the original radiation dose. Since most animal tumors show a nonviable or necrotic volume, a description of the phenomenon was given for the C3H/Bi mammary carcinoma by noting that the percent of nonviable tissue approaches a limiting (asymptomic)value.", "PMID": 976766}
{"id": "pubmed23n0032_10873", "title": "Estimation of growth velocities from individual longitudinal data.", "content": "Since the construction of growth velocity curves using the simple increment method (i.e., plotting the increments deltaxt vs, time, t) involves a number of methodological problems, when mathematical curves are fit, they are generally fit to the \"distance traveled\", plot (of xt vs. t) and the corresponding velocity curve is then obtained by differentiation. However, most of the traditional methods for accomplishing this (e.g., fitting the Gompertz or logistic curves) require that the course of growth be studied over a sufficiently long time period to allow accurate determinations of the upper and lower asymptotes of these S-shaped functions. This is not always feasible in practice: a case in point being the mixed-longitudinal Nijmegen Growth Study where each of the cohorts comprising the sample is followed for but a five-year period. In such situations, alternative approaches to the problem of constructing growth velocity curves may be of considerable practical as well as theoretical interest. One such approach is developed in this paper and its use is illustrated on some data collected as part of the Nijmegen Growth Study. These results are then compared with those obtained using the increment method on the same data.", "contents": "Estimation of growth velocities from individual longitudinal data. Since the construction of growth velocity curves using the simple increment method (i.e., plotting the increments deltaxt vs, time, t) involves a number of methodological problems, when mathematical curves are fit, they are generally fit to the \"distance traveled\", plot (of xt vs. t) and the corresponding velocity curve is then obtained by differentiation. However, most of the traditional methods for accomplishing this (e.g., fitting the Gompertz or logistic curves) require that the course of growth be studied over a sufficiently long time period to allow accurate determinations of the upper and lower asymptotes of these S-shaped functions. This is not always feasible in practice: a case in point being the mixed-longitudinal Nijmegen Growth Study where each of the cohorts comprising the sample is followed for but a five-year period. In such situations, alternative approaches to the problem of constructing growth velocity curves may be of considerable practical as well as theoretical interest. One such approach is developed in this paper and its use is illustrated on some data collected as part of the Nijmegen Growth Study. These results are then compared with those obtained using the increment method on the same data.", "PMID": 976767}
{"id": "pubmed23n0032_10874", "title": "Histological and histochemical investigations of achondroplastic mice: a possible model of human achondroplasia.", "content": "Histological and histochemical investigations of tibial epiphyses, costo-chondral junctions and caudal vertebrae of achondroplastic (cn) mice have shown that, in spite of conspicuous reduction of bone length, endochondrial ossification occurs in much the same way as in controls. Moreover, the histological structure of seriated cartilage, and the distribution of proteoglycans in resting cartilage are the same in cn/cn mice and in controls. The only difference betweeen the two types of animals is represented by the occurence of early aging-like changes of chondrocytes and cartilage matrix in achondroplastic mice, leading to premature shortening of the cell columns and to early reduction of the proteoglycan concentration. The premature \"aging\" of the cartilage, the consequent inhibition of the calcification process and bone growth, and the normal rate of perichondral ossification give to long bones, vertebrae and ribs a typical achondroplastic appearance. The cn/cn mice seem to represent a useful model for studying the pathogenesis and therapy of human achondroplasia.", "contents": "Histological and histochemical investigations of achondroplastic mice: a possible model of human achondroplasia. Histological and histochemical investigations of tibial epiphyses, costo-chondral junctions and caudal vertebrae of achondroplastic (cn) mice have shown that, in spite of conspicuous reduction of bone length, endochondrial ossification occurs in much the same way as in controls. Moreover, the histological structure of seriated cartilage, and the distribution of proteoglycans in resting cartilage are the same in cn/cn mice and in controls. The only difference betweeen the two types of animals is represented by the occurence of early aging-like changes of chondrocytes and cartilage matrix in achondroplastic mice, leading to premature shortening of the cell columns and to early reduction of the proteoglycan concentration. The premature \"aging\" of the cartilage, the consequent inhibition of the calcification process and bone growth, and the normal rate of perichondral ossification give to long bones, vertebrae and ribs a typical achondroplastic appearance. The cn/cn mice seem to represent a useful model for studying the pathogenesis and therapy of human achondroplasia.", "PMID": 976768}
{"id": "pubmed23n0032_10875", "title": "Muscle growth in two genetically different lines of swine.", "content": "Skeletal muscle growth in two genetic lines of pigs that differed in total muscle content was studied at live weights of 23, 45, 68, 91, 104, 118 kg. Total physically separable muscle and cross-sectional area of the longissimus dorsi muscle were greater in the muscular than in the obese genetic lines. Above 45 kg, animals in the muscular genetic line had less total separable fat than animals in the obese line, but the two lines did not differ in total physically separable fat at 23 and 45 kg live weight. Hence, these two genetic lines may differ in weight at which maturity is reached as much as in inherent propensity for obesity. Longissimus muscle form the muscular line had more water, less protein, and less lipid than longissimus from the obese line. DNA and RNA concentration, total DNA and RNA content, and RNA/DNA ratio of the pituitary and liver did not differ between the two genetic lines. Above 68 kg, longissimus from the muscular line had higher DNA and RNA concentrations than longissimus from the obese line; this difference did not exist between 23 and 68 kg. RNA/DNA ratio of the longissimus muscle was greater and protein-to-DNA and protein-to-RNA ratios in longissimus were lower in the muscular than in the obese line. Total DNA content of physically separable muscle increased 2.0 (obese) to 2.7 (muscular)-fold between 23 and 118 kg; hence, number of muscle nuclei increases during growth. Total DNA content of physically separable muscle was greater in the muscular than in the obese line and was the measurement most highly related to total muscle content.", "contents": "Muscle growth in two genetically different lines of swine. Skeletal muscle growth in two genetic lines of pigs that differed in total muscle content was studied at live weights of 23, 45, 68, 91, 104, 118 kg. Total physically separable muscle and cross-sectional area of the longissimus dorsi muscle were greater in the muscular than in the obese genetic lines. Above 45 kg, animals in the muscular genetic line had less total separable fat than animals in the obese line, but the two lines did not differ in total physically separable fat at 23 and 45 kg live weight. Hence, these two genetic lines may differ in weight at which maturity is reached as much as in inherent propensity for obesity. Longissimus muscle form the muscular line had more water, less protein, and less lipid than longissimus from the obese line. DNA and RNA concentration, total DNA and RNA content, and RNA/DNA ratio of the pituitary and liver did not differ between the two genetic lines. Above 68 kg, longissimus from the muscular line had higher DNA and RNA concentrations than longissimus from the obese line; this difference did not exist between 23 and 68 kg. RNA/DNA ratio of the longissimus muscle was greater and protein-to-DNA and protein-to-RNA ratios in longissimus were lower in the muscular than in the obese line. Total DNA content of physically separable muscle increased 2.0 (obese) to 2.7 (muscular)-fold between 23 and 118 kg; hence, number of muscle nuclei increases during growth. Total DNA content of physically separable muscle was greater in the muscular than in the obese line and was the measurement most highly related to total muscle content.", "PMID": 976769}
{"id": "pubmed23n0032_10876", "title": "Effect of growth and plane of nutrition on apparent muscle fiber numbers in the pig.", "content": "A histological sampling technique was used to estimate the number of muscle fibers appearing in transverse sections of whole sartorius muscles. Transverse sections were taken at the midlength of sartorius muscles from pigs growing at a rate of approximately 0.7 kg/day. Between 56 and 168 days (postnatal), the apparent number of muscle fibers increased at an approximate rate of 300 fibers/day. No evidence of the formation of new muscle fibers by myoblast fusion or muscle fiber fission was detected by light microscopy. Animals placed on a maintenance diet which prevented growth for 28-day periods prior to slaughter showed a fixation or decrease in apparent numbers of muscle fibers. These data support the hypothesis that the arrangement of muscle fibers during growth of the porcine sartorius muscle is a dynamic rather than a fixed system.", "contents": "Effect of growth and plane of nutrition on apparent muscle fiber numbers in the pig. A histological sampling technique was used to estimate the number of muscle fibers appearing in transverse sections of whole sartorius muscles. Transverse sections were taken at the midlength of sartorius muscles from pigs growing at a rate of approximately 0.7 kg/day. Between 56 and 168 days (postnatal), the apparent number of muscle fibers increased at an approximate rate of 300 fibers/day. No evidence of the formation of new muscle fibers by myoblast fusion or muscle fiber fission was detected by light microscopy. Animals placed on a maintenance diet which prevented growth for 28-day periods prior to slaughter showed a fixation or decrease in apparent numbers of muscle fibers. These data support the hypothesis that the arrangement of muscle fibers during growth of the porcine sartorius muscle is a dynamic rather than a fixed system.", "PMID": 976770}
{"id": "pubmed23n0032_10877", "title": "Serum cholesterol, triglyceride, glucose and total bilirubin concentrations, as functions of age and sex, in Sinclair(S-1) miniature swine.", "content": "Venous blood samples were collected monthly from 60 male and 60 female Sinclair (S-1) miniature swine from 1 through 36 months of age. Serum cholesterol concentration decreased from 5 through 36 months of age. Also, gilts had a greater mean serum cholesterol concentration than did boars. Mean serum triglyceride concentration was greater for females than for males. From 1 through 15 months of age, serum glucose concentration decreased with no sex related difference. From 1 1/2 to 3 years of age, gilts had greater mean serum glucose concentrations than did boars. Throughout the longitudinal study, mean serum total bilirubin concentrations were similar for females and males, with no age related variations.", "contents": "Serum cholesterol, triglyceride, glucose and total bilirubin concentrations, as functions of age and sex, in Sinclair(S-1) miniature swine. Venous blood samples were collected monthly from 60 male and 60 female Sinclair (S-1) miniature swine from 1 through 36 months of age. Serum cholesterol concentration decreased from 5 through 36 months of age. Also, gilts had a greater mean serum cholesterol concentration than did boars. Mean serum triglyceride concentration was greater for females than for males. From 1 through 15 months of age, serum glucose concentration decreased with no sex related difference. From 1 1/2 to 3 years of age, gilts had greater mean serum glucose concentrations than did boars. Throughout the longitudinal study, mean serum total bilirubin concentrations were similar for females and males, with no age related variations.", "PMID": 976771}
{"id": "pubmed23n0032_10878", "title": "Ascorbic acid deficiency in liver disease.", "content": "Leucocyte ascorbic acid (LAA) levels were measured in 138 patients with liver disease. Significantly reduced levels were found in 37 patients with alcoholic liver disease (P less than 0-01) and 25 patients with primary biliary cirrhosis (P less than 0-05). In the primary biliary cirrhosis patients, cholestyramine therapy was associated with significantly lower levels of the vitamin (P less than 0-05). Liver ascorbic acid measured in Menghini needle biopsies in 20 patients was significantly correlated with LAA (r=0-807, P less than 0-001). No significant correlation was found between LAA and haematological indices, conventional liver function tests, or cholesterol levels in any group of patients. Patients with LAA levels below 100 nM/10(8) WBC had significantly higher antipyrine half-lives (mean=28-3 h) than patients with LAA levels above this level (mean=18-6 h) (P less than 0-05). Delayed drug metabolism related to low LAA should be considered when drugs metabolised by the liver are prescribed for patients with alcoholic liver disease or primary biliary cirrhosis.", "contents": "Ascorbic acid deficiency in liver disease. Leucocyte ascorbic acid (LAA) levels were measured in 138 patients with liver disease. Significantly reduced levels were found in 37 patients with alcoholic liver disease (P less than 0-01) and 25 patients with primary biliary cirrhosis (P less than 0-05). In the primary biliary cirrhosis patients, cholestyramine therapy was associated with significantly lower levels of the vitamin (P less than 0-05). Liver ascorbic acid measured in Menghini needle biopsies in 20 patients was significantly correlated with LAA (r=0-807, P less than 0-001). No significant correlation was found between LAA and haematological indices, conventional liver function tests, or cholesterol levels in any group of patients. Patients with LAA levels below 100 nM/10(8) WBC had significantly higher antipyrine half-lives (mean=28-3 h) than patients with LAA levels above this level (mean=18-6 h) (P less than 0-05). Delayed drug metabolism related to low LAA should be considered when drugs metabolised by the liver are prescribed for patients with alcoholic liver disease or primary biliary cirrhosis.", "PMID": 976794}
{"id": "pubmed23n0032_10879", "title": "Alteration of drug metabolism in Gilbert's syndrome.", "content": "The pathophysiology of Gilbert's syndrome was studied by investigating the metabolism of the drug tolbutamide, which is metabolised by the liver but does not undergo glucuronidation. Using rat liver cell supernatant, tolbutamide was shown to bind to the hepatic cytoplasmic Y protein in a manner similar to other organic anions, but not to Z protein. In 31 patients with Gilbert's syndrome the plasma disappearance (plasma half-life, mean +/- SD: 628+/-84 min) and metabolic clearance (7-9+/-1-8 ml/min) were significantly (P less than 0-0005) altered compared with the 13 controls (mean half-life 393+/-26 and mean clearance 13-4+/-1-5). The eight patients with hyperbilirubinaemia due to haemolytic disease showed no difference from the normal control subjects. In three patients with Gilbert's syndrome the cumulative urinary excretion of tolbutamide metabolites, 24 hours after the administration of the drug, was 30% lower than in the controls. In the five patients with Gilbert's syndrome, phenobarbital administration (100 mg/day) produced a significant increase in clearance of the drug from 8-8+/-0-8 to 13-4+/-1-9 ml/min; this was paralleled by a fall in serum bilirubin concentration. The plasma half-life of tolbutamide was similar in Gunn rats and Wistar rats. The results suggest that the metabolic defect(s) of Gilbert's syndrome affects compounds other than bilirubin and that defective uptake is probably the major factor.", "contents": "Alteration of drug metabolism in Gilbert's syndrome. The pathophysiology of Gilbert's syndrome was studied by investigating the metabolism of the drug tolbutamide, which is metabolised by the liver but does not undergo glucuronidation. Using rat liver cell supernatant, tolbutamide was shown to bind to the hepatic cytoplasmic Y protein in a manner similar to other organic anions, but not to Z protein. In 31 patients with Gilbert's syndrome the plasma disappearance (plasma half-life, mean +/- SD: 628+/-84 min) and metabolic clearance (7-9+/-1-8 ml/min) were significantly (P less than 0-0005) altered compared with the 13 controls (mean half-life 393+/-26 and mean clearance 13-4+/-1-5). The eight patients with hyperbilirubinaemia due to haemolytic disease showed no difference from the normal control subjects. In three patients with Gilbert's syndrome the cumulative urinary excretion of tolbutamide metabolites, 24 hours after the administration of the drug, was 30% lower than in the controls. In the five patients with Gilbert's syndrome, phenobarbital administration (100 mg/day) produced a significant increase in clearance of the drug from 8-8+/-0-8 to 13-4+/-1-9 ml/min; this was paralleled by a fall in serum bilirubin concentration. The plasma half-life of tolbutamide was similar in Gunn rats and Wistar rats. The results suggest that the metabolic defect(s) of Gilbert's syndrome affects compounds other than bilirubin and that defective uptake is probably the major factor.", "PMID": 976795}
{"id": "pubmed23n0032_10880", "title": "Marked delay in indocyanine green plasma clearance with a near-normal bromosulphophthalein retention test: a constitutional abnormality?", "content": "Five patients showing a normal to near-normal BSP test and a marked delay in ICG plasma clearance, and two families with clustering of the same abnormality, are described. Two had Gilbert's syndrome, one was convalescing from acute hepatitis, and the other two had no detectable liver abnormality. Measured indices of ICG metabolism indicated a marked reduction in the hepatic uptake, storage capacity, biliary transport maximum, and an increased reflux into plasma. Biochemical studies on the binding of ICG by plasma proteins failed to demonstrate any difference from normal controls. Thus, the primary defect seems to be in the transport of ICG by the hepatocyte. Although the relationship of the defect in ICG metabolism to other constitutional hyperbilirubinaemias is not clear, it is possible that the defect in these patients is a constitutional one involving some steps in the hepatic disposal of organic anions.", "contents": "Marked delay in indocyanine green plasma clearance with a near-normal bromosulphophthalein retention test: a constitutional abnormality? Five patients showing a normal to near-normal BSP test and a marked delay in ICG plasma clearance, and two families with clustering of the same abnormality, are described. Two had Gilbert's syndrome, one was convalescing from acute hepatitis, and the other two had no detectable liver abnormality. Measured indices of ICG metabolism indicated a marked reduction in the hepatic uptake, storage capacity, biliary transport maximum, and an increased reflux into plasma. Biochemical studies on the binding of ICG by plasma proteins failed to demonstrate any difference from normal controls. Thus, the primary defect seems to be in the transport of ICG by the hepatocyte. Although the relationship of the defect in ICG metabolism to other constitutional hyperbilirubinaemias is not clear, it is possible that the defect in these patients is a constitutional one involving some steps in the hepatic disposal of organic anions.", "PMID": 976796}
{"id": "pubmed23n0032_10881", "title": "Disappearance half-time of endogenous and exogenous secretin in dogs.", "content": "We have used a sensitive and specific radioimmunoassay to measurespectively. the disappearance half-times of exogenous porcine secretin and endogenous canine secretin in the dog and found them to be 2-45 and 2-85 minutes, respectively.", "contents": "Disappearance half-time of endogenous and exogenous secretin in dogs. We have used a sensitive and specific radioimmunoassay to measurespectively. the disappearance half-times of exogenous porcine secretin and endogenous canine secretin in the dog and found them to be 2-45 and 2-85 minutes, respectively.", "PMID": 976797}
{"id": "pubmed23n0032_10882", "title": "Evaluation of the intraepithelial lymphocyte count in the jejunum in childhood enteropathies.", "content": "Intraepithelial lymphocyte counts were evaluated in 131 jejunal mucosal biopsies taken from children with a small intestinal enteropathy arising from a variety of causes including coeliac disease, (untreated, after gluten withdrawal, and during subsequent challenge), giardiasis, cow's milk protein intolerance, and 'intractable diarrhoea'. The counts were compared with those from the biopsies of children referred for investigation but in whom no gastrointestinal disease was demonstrated and from healthy siblings of children with coeliac disease, investigated during a family study. Children with coeliac disease showed a raised count which fell after gluten withdrawal as has been demonstrated by others in adults. Lymphocytic infiltration of the epithelium increased rapidly during gluten challenge in such children, while no change was seen in those children proven ultimately not to have coeliac disease by the usually recognized criteria. In other enteropathies the range of counts was wide, overlapping with both normal and coeliac groups and indicating the nonspecificity of lymphocytic infiltration of the gut epithelium. The findings are discussed in relation to their significance and to further avenues of investigation to determine their possible diagnostic value in confirming the diagnosis of coeliac disease during gluten challenge.", "contents": "Evaluation of the intraepithelial lymphocyte count in the jejunum in childhood enteropathies. Intraepithelial lymphocyte counts were evaluated in 131 jejunal mucosal biopsies taken from children with a small intestinal enteropathy arising from a variety of causes including coeliac disease, (untreated, after gluten withdrawal, and during subsequent challenge), giardiasis, cow's milk protein intolerance, and 'intractable diarrhoea'. The counts were compared with those from the biopsies of children referred for investigation but in whom no gastrointestinal disease was demonstrated and from healthy siblings of children with coeliac disease, investigated during a family study. Children with coeliac disease showed a raised count which fell after gluten withdrawal as has been demonstrated by others in adults. Lymphocytic infiltration of the epithelium increased rapidly during gluten challenge in such children, while no change was seen in those children proven ultimately not to have coeliac disease by the usually recognized criteria. In other enteropathies the range of counts was wide, overlapping with both normal and coeliac groups and indicating the nonspecificity of lymphocytic infiltration of the gut epithelium. The findings are discussed in relation to their significance and to further avenues of investigation to determine their possible diagnostic value in confirming the diagnosis of coeliac disease during gluten challenge.", "PMID": 976798}
{"id": "pubmed23n0032_10883", "title": "Absorption of xylose, glucose, glycine, and folic (pteroylglutamic) acid in Zambian Africans with anaemia.", "content": "Rates of glucose, glycine, and folic (pteroylglutamic) acid absorption were determined for a 30 cm jejunal segment in vivo, with a double-lumen tube perfusion system, in 10 Zambian African women with a mean haemoglobin concentration of 5-1 (3-5-9-2) g/dl. In four the anaemia was megaloblastic (due to folate deficiency) and in six hypochromic. Perfusion solutions contained (1) glucose 200 mmol/1, (2) glycine 100 mmol/1, and (3) folic acid 250 mug/1. D-xylose absorption after a 25 g oral load was determined in them, and also in 18 additional patients (11 had megaloblastic and seven either hypochromic or haemolytic anaemia). Xylose absorption tests were significantly impaired in the patients with megaloblastic compared with hypochromic or haemolytic anaemia (P less than 0-001); those with untreated megaloblastic anaemia had a greater abnormality than those who had started treatment. Mean glucose, glycine, and folic acid absorption rates were similar to those in controls, and the rates in patients with megaloblastic and hypochromic anaemia were not significantly different. Correlation between glucose absorption rate and xylose excretion was, however, significantly (P less than 0-02). If more patients had been studied it seems likely therefore that a significant impairment of glucose absorption rate in the presence of megaloblastic anaemia would also have been demonstrated. In this investigation anaemia per se did not affect glucose, glycine, or folic acid absorption rates or xylose absorption, but xylose absorption was reduced in patients with megaloblastic anaemia. That abnormality was probably related to folate deficiency, and the underlying mechanism seems to be different from that causing impairment of monosaccharide absorption in patients with systemic bacterial infections. Mean glycine and folic acid absorption rates were not altered by megaloblastic anaemia, indicating that folate deficiency does not cause a general depression of absorption.", "contents": "Absorption of xylose, glucose, glycine, and folic (pteroylglutamic) acid in Zambian Africans with anaemia. Rates of glucose, glycine, and folic (pteroylglutamic) acid absorption were determined for a 30 cm jejunal segment in vivo, with a double-lumen tube perfusion system, in 10 Zambian African women with a mean haemoglobin concentration of 5-1 (3-5-9-2) g/dl. In four the anaemia was megaloblastic (due to folate deficiency) and in six hypochromic. Perfusion solutions contained (1) glucose 200 mmol/1, (2) glycine 100 mmol/1, and (3) folic acid 250 mug/1. D-xylose absorption after a 25 g oral load was determined in them, and also in 18 additional patients (11 had megaloblastic and seven either hypochromic or haemolytic anaemia). Xylose absorption tests were significantly impaired in the patients with megaloblastic compared with hypochromic or haemolytic anaemia (P less than 0-001); those with untreated megaloblastic anaemia had a greater abnormality than those who had started treatment. Mean glucose, glycine, and folic acid absorption rates were similar to those in controls, and the rates in patients with megaloblastic and hypochromic anaemia were not significantly different. Correlation between glucose absorption rate and xylose excretion was, however, significantly (P less than 0-02). If more patients had been studied it seems likely therefore that a significant impairment of glucose absorption rate in the presence of megaloblastic anaemia would also have been demonstrated. In this investigation anaemia per se did not affect glucose, glycine, or folic acid absorption rates or xylose absorption, but xylose absorption was reduced in patients with megaloblastic anaemia. That abnormality was probably related to folate deficiency, and the underlying mechanism seems to be different from that causing impairment of monosaccharide absorption in patients with systemic bacterial infections. Mean glycine and folic acid absorption rates were not altered by megaloblastic anaemia, indicating that folate deficiency does not cause a general depression of absorption.", "PMID": 976799}
{"id": "pubmed23n0032_10884", "title": "Human calcitonin stimulates salivary amylase output in man.", "content": "Salivary amylase output in man increases after injection of synthetic human calcitonin. This effect is dose dependent.", "contents": "Human calcitonin stimulates salivary amylase output in man. Salivary amylase output in man increases after injection of synthetic human calcitonin. This effect is dose dependent.", "PMID": 976800}
{"id": "pubmed23n0032_10885", "title": "Adenocarcinoma of the stomach: radiological and pathological correlation of effects of treatment with fast neutrons.", "content": "During the past six years 40 patients have been treated with fast neutrons for inoperable adenocarcinoma of the stomach. A number of these patients have been studied in detail both radiologically and pathologically with regard to the effects of fast neutrons on both the tumour and normal stomach. After treatment there was a constant reduction in size of the stomach capacity when compared with a control group and the motility of the stomach was lost. Mucosal changes, which were noted radiologically, appeared to be dose related. Histologically most of the tumour was destroyed. Small clumps of cells were, however, found in all except one of the patients but only in areas of the stomach which had not received the standard dose. Marked fibrosis occurred and it is our impression that the amount of fibrosis is in excess of that seen with other forms of radiotherapy.", "contents": "Adenocarcinoma of the stomach: radiological and pathological correlation of effects of treatment with fast neutrons. During the past six years 40 patients have been treated with fast neutrons for inoperable adenocarcinoma of the stomach. A number of these patients have been studied in detail both radiologically and pathologically with regard to the effects of fast neutrons on both the tumour and normal stomach. After treatment there was a constant reduction in size of the stomach capacity when compared with a control group and the motility of the stomach was lost. Mucosal changes, which were noted radiologically, appeared to be dose related. Histologically most of the tumour was destroyed. Small clumps of cells were, however, found in all except one of the patients but only in areas of the stomach which had not received the standard dose. Marked fibrosis occurred and it is our impression that the amount of fibrosis is in excess of that seen with other forms of radiotherapy.", "PMID": 976801}
{"id": "pubmed23n0032_10886", "title": "Multiple recurring gastric erosions/aphthous ulcers).", "content": "Five patients are described who had repeated endoscopy because of continuing dyspeptic symptoms associated with a negative barium meal. They were found to have multiple recurring gastric erosions (aphthous ulcers). No common aetiological factor could be found, although four of these patients did have a mild or moderatley active superficial chronic gastritis. Conventional peptic ulcer therapy failed to control either symptoms or ulceration. Two patients finally came to gastric surgery (highly selective vagotomy), which resulted in the relief of symptoms and healing of the gastric aphthous ulceration.", "contents": "Multiple recurring gastric erosions/aphthous ulcers). Five patients are described who had repeated endoscopy because of continuing dyspeptic symptoms associated with a negative barium meal. They were found to have multiple recurring gastric erosions (aphthous ulcers). No common aetiological factor could be found, although four of these patients did have a mild or moderatley active superficial chronic gastritis. Conventional peptic ulcer therapy failed to control either symptoms or ulceration. Two patients finally came to gastric surgery (highly selective vagotomy), which resulted in the relief of symptoms and healing of the gastric aphthous ulceration.", "PMID": 976802}
{"id": "pubmed23n0032_10887", "title": "Measurement of anal pressure and motility.", "content": "A fine open perfused system and a closed balloon system for the measurement of anal pressure and motility have been compared. Measurements were made in 40 normal subjects and 84 patients with haemorrhoids. The rate of perfusion had a marked effect on the recorded pressure and motility details. The motility pattern was seen most clearly with the balloon probe and the pressure recorded was reproducible and easy to measure, making this a convenient method for recording activity of the internal anal sphincter. Anal motility in normal subjects was characterised by slow pressure waves (10-20/min). The frequency was fastest in the distal anal canal and this frequency gradient may represent a normal mechanism to keep the anal canal empty. Ultra slow pressure waves (0-6-1-9/min) were seen in 42% of patients with haemorrhoids and 5% of normal subjects and arose from a synchronous contraction of the whole internal sphincter.", "contents": "Measurement of anal pressure and motility. A fine open perfused system and a closed balloon system for the measurement of anal pressure and motility have been compared. Measurements were made in 40 normal subjects and 84 patients with haemorrhoids. The rate of perfusion had a marked effect on the recorded pressure and motility details. The motility pattern was seen most clearly with the balloon probe and the pressure recorded was reproducible and easy to measure, making this a convenient method for recording activity of the internal anal sphincter. Anal motility in normal subjects was characterised by slow pressure waves (10-20/min). The frequency was fastest in the distal anal canal and this frequency gradient may represent a normal mechanism to keep the anal canal empty. Ultra slow pressure waves (0-6-1-9/min) were seen in 42% of patients with haemorrhoids and 5% of normal subjects and arose from a synchronous contraction of the whole internal sphincter.", "PMID": 976803}
{"id": "pubmed23n0032_10888", "title": "An apparatus for the anaerobic ultrafiltration of bile and other biological fluids.", "content": "A simple apparatus is described for the anaerobic ultrafiltration of a few millilitres of bile or other biological fluids. The principle employed enables viscous liquids which would normally block the membrane to be ultrafiltered successfully.", "contents": "An apparatus for the anaerobic ultrafiltration of bile and other biological fluids. A simple apparatus is described for the anaerobic ultrafiltration of a few millilitres of bile or other biological fluids. The principle employed enables viscous liquids which would normally block the membrane to be ultrafiltered successfully.", "PMID": 976804}
{"id": "pubmed23n0032_10889", "title": "Neuroleptanalgesia in upper alimentary endoscopy.", "content": "Droperidol and fentanyl have been used with diazepam in 250 peroral endoscopies because of dissatisfaction with standard methods of sedation. Excellant analgesia has been achieved and complications have been negligible. The technique does not require the presence of an anaesthetist.", "contents": "Neuroleptanalgesia in upper alimentary endoscopy. Droperidol and fentanyl have been used with diazepam in 250 peroral endoscopies because of dissatisfaction with standard methods of sedation. Excellant analgesia has been achieved and complications have been negligible. The technique does not require the presence of an anaesthetist.", "PMID": 976805}
{"id": "pubmed23n0032_10890", "title": "Histopathology of cell mediated immune reaction in mouse colon--allograft rejection.", "content": "Grafts of mouse fetal colon, implanted beneath the renal capsule of adult hosts, have been used to study the growth and development of colonic isografts and the rejection of colonic allografts. Isografts grew normally and maintained a structure similar to normal colon. Grafts between strains with H2 histocompatibility differences were rejected by 13 days after transplantation. Early progressive infiltration of the grafts by lymphoid cells was followed by increasing damage to, and subsequent loss of, the epithelial cell layer and destruction of the underlying muscle, changes which parallel those seen in rejection of skin and small bowel. The increase in survival time which is seen in allografts between strains with H2 identity was longer in the colon than has been seen in the skin or small bowel; none of the allografts of colon were completely rejected before 30 days, and some remained viable at 50 days. Comparison of the appearances of rejection in the colon with those of ulcerative colitis and colonic Crohn's disease does not show the striking similarity which is seen between small bowel rejection and coeliac disease. Many of the individual features of these diseases are, however, present in the course of colonic rejection.", "contents": "Histopathology of cell mediated immune reaction in mouse colon--allograft rejection. Grafts of mouse fetal colon, implanted beneath the renal capsule of adult hosts, have been used to study the growth and development of colonic isografts and the rejection of colonic allografts. Isografts grew normally and maintained a structure similar to normal colon. Grafts between strains with H2 histocompatibility differences were rejected by 13 days after transplantation. Early progressive infiltration of the grafts by lymphoid cells was followed by increasing damage to, and subsequent loss of, the epithelial cell layer and destruction of the underlying muscle, changes which parallel those seen in rejection of skin and small bowel. The increase in survival time which is seen in allografts between strains with H2 identity was longer in the colon than has been seen in the skin or small bowel; none of the allografts of colon were completely rejected before 30 days, and some remained viable at 50 days. Comparison of the appearances of rejection in the colon with those of ulcerative colitis and colonic Crohn's disease does not show the striking similarity which is seen between small bowel rejection and coeliac disease. Many of the individual features of these diseases are, however, present in the course of colonic rejection.", "PMID": 976806}
{"id": "pubmed23n0032_10891", "title": "Ischaemic colitis in the experimental animal. II. Role of hypovolaemia in the production of the disease.", "content": "Hypovolaemia alone did not lead to ischaemic colitis but when venesection was induced immediately after the acute ligation of the common colic artery large bowel ischaemia ensued. Similarly, hypovolaemia induced one month after two major blood vessels had been occluded led to ischaemic colitis. These findings suggest that states of low blood flow in the presence of previous arterial constriction or blockage may lead to enough reduction in mesenteric perfusion for intestinal ischaemia to develop. Using an electromagnetic flowmeter placed in the cranial mesenteric artery of the dog, it was shown that hypovolaemia may lead to 50-75% reduction in mesenteric blood flow without producing any significant change in the systemic blood pressure.", "contents": "Ischaemic colitis in the experimental animal. II. Role of hypovolaemia in the production of the disease. Hypovolaemia alone did not lead to ischaemic colitis but when venesection was induced immediately after the acute ligation of the common colic artery large bowel ischaemia ensued. Similarly, hypovolaemia induced one month after two major blood vessels had been occluded led to ischaemic colitis. These findings suggest that states of low blood flow in the presence of previous arterial constriction or blockage may lead to enough reduction in mesenteric perfusion for intestinal ischaemia to develop. Using an electromagnetic flowmeter placed in the cranial mesenteric artery of the dog, it was shown that hypovolaemia may lead to 50-75% reduction in mesenteric blood flow without producing any significant change in the systemic blood pressure.", "PMID": 976807}
{"id": "pubmed23n0032_10892", "title": "Exocrine pancreatic function in juvenile-onset diabetes mellitus.", "content": "Exocrine pancreatic function was studied in 20 juvenile-onset diabetics, seven maturity-onset diabetics, and five patients with diabetes secondary to chronic pancreatitis. The results were compared with 13 non-diabetic controls. The outputs of bicarbonate, trypsin, and amylase were reduced in the diabetic patients in response to intravenous secretin and CCK-PZ. In the juvenile-onset group, exocrine pancreatic secretory capacity was reduced in 80% of the patients, and the severity of the reduction was related to the duration of the diabetes. The reduction in pancreatic secretory capacity must be taken into consideration when interpreting pancreatic exocrine function in patients with diabetes.", "contents": "Exocrine pancreatic function in juvenile-onset diabetes mellitus. Exocrine pancreatic function was studied in 20 juvenile-onset diabetics, seven maturity-onset diabetics, and five patients with diabetes secondary to chronic pancreatitis. The results were compared with 13 non-diabetic controls. The outputs of bicarbonate, trypsin, and amylase were reduced in the diabetic patients in response to intravenous secretin and CCK-PZ. In the juvenile-onset group, exocrine pancreatic secretory capacity was reduced in 80% of the patients, and the severity of the reduction was related to the duration of the diabetes. The reduction in pancreatic secretory capacity must be taken into consideration when interpreting pancreatic exocrine function in patients with diabetes.", "PMID": 976808}
{"id": "pubmed23n0032_10893", "title": "Stenosis of the colon in acute pancreatitis.", "content": "A case is described demonstrating a stricture of the colon associated with acute pancreatitis and 20 similar cases found in the literature are reviewed. The colonic stenosis may be of short duration and largely due to oedema or more chronic with deposition of fibrous tissue. Many cases have been confused with a carcinoma both radiologically and at laparotomy. It is suggested that a conservative approach be adopted initially, resection being carried out if follow-up barium examination fails to show resolution of the stenosis.", "contents": "Stenosis of the colon in acute pancreatitis. A case is described demonstrating a stricture of the colon associated with acute pancreatitis and 20 similar cases found in the literature are reviewed. The colonic stenosis may be of short duration and largely due to oedema or more chronic with deposition of fibrous tissue. Many cases have been confused with a carcinoma both radiologically and at laparotomy. It is suggested that a conservative approach be adopted initially, resection being carried out if follow-up barium examination fails to show resolution of the stenosis.", "PMID": 976809}
{"id": "pubmed23n0032_10894", "title": "Ferritin in Crohn's disease tissue: detection by electron microscopy.", "content": "Using immune electron microscopy, an attempt has been made to visualise viral particles in Crohn's disease tissue and faeces. No particles resembling viruses were observed, but in the absence of a specific antiserum a negative result does not exclude the presence of a virus, particularly if it is sparsely distributed. Compared with controls, an abundance of a 12 nm particle was found in all Crohn's disease tissue and one small intestinal lymphoma. This particle has been identified as ferritin and is unlikely to be of aetiological significance.", "contents": "Ferritin in Crohn's disease tissue: detection by electron microscopy. Using immune electron microscopy, an attempt has been made to visualise viral particles in Crohn's disease tissue and faeces. No particles resembling viruses were observed, but in the absence of a specific antiserum a negative result does not exclude the presence of a virus, particularly if it is sparsely distributed. Compared with controls, an abundance of a 12 nm particle was found in all Crohn's disease tissue and one small intestinal lymphoma. This particle has been identified as ferritin and is unlikely to be of aetiological significance.", "PMID": 976810}
{"id": "pubmed23n0032_10895", "title": "Hypergastrinaemia in cirrhosis of liver.", "content": "The basal acid output (BAO), post-pentagastrin acid output (MAO), fasting and post-prandial gastrin levels in 40 patients with proven cirrhosis of the liver were compared with those in 20 normal controls. The mean BAO and MAO were significantly lower than normal, the mean fasting gastrin level was significantly higher than normal, and the postprandial gastrin response was significantly increased and prolonged. These differences were still significant even when the patients were divided into cryptogenic and alcoholic subgroups. A significant inverse relationship between MAO and the integrated gastrin response to meal was observed both in the normal controls and in the cirrhotic patients. The MAO and integrated gastrin response of the cirrhotic patients did not correlate with the degree of liver function impairment. In five cirrhotic patients fasting and postprandial gastrin levels were unchanged after splenorenal shunt operation. A more consistent abnormality of the gastric mucosa as assessed by endoscopy and biopsies appeared to be mucosal congestion with occasional atrophic gastritis. the severity of mucosal abnormality, however, was unrelated to the degree of hypoacidity. these results indicate, firstly, that the hypergastrinaemia in cirrhotic patients is a reflection of gastric hypoacidity and bears no direct relationship to hepatic dysfunction. Secondly, the gastric hypoacidity does not accrue solely from mucosal abnormality. It is suggested that this hypoacidity may result from the presence of excessive amounts of circulating acid-inhibiting intestinal peptides, which the diseased liver fails to metabolise.", "contents": "Hypergastrinaemia in cirrhosis of liver. The basal acid output (BAO), post-pentagastrin acid output (MAO), fasting and post-prandial gastrin levels in 40 patients with proven cirrhosis of the liver were compared with those in 20 normal controls. The mean BAO and MAO were significantly lower than normal, the mean fasting gastrin level was significantly higher than normal, and the postprandial gastrin response was significantly increased and prolonged. These differences were still significant even when the patients were divided into cryptogenic and alcoholic subgroups. A significant inverse relationship between MAO and the integrated gastrin response to meal was observed both in the normal controls and in the cirrhotic patients. The MAO and integrated gastrin response of the cirrhotic patients did not correlate with the degree of liver function impairment. In five cirrhotic patients fasting and postprandial gastrin levels were unchanged after splenorenal shunt operation. A more consistent abnormality of the gastric mucosa as assessed by endoscopy and biopsies appeared to be mucosal congestion with occasional atrophic gastritis. the severity of mucosal abnormality, however, was unrelated to the degree of hypoacidity. these results indicate, firstly, that the hypergastrinaemia in cirrhotic patients is a reflection of gastric hypoacidity and bears no direct relationship to hepatic dysfunction. Secondly, the gastric hypoacidity does not accrue solely from mucosal abnormality. It is suggested that this hypoacidity may result from the presence of excessive amounts of circulating acid-inhibiting intestinal peptides, which the diseased liver fails to metabolise.", "PMID": 976811}
{"id": "pubmed23n0032_10896", "title": "Idiopathic cholestasis of pregnancy in a large kindred.", "content": "A past history of idiopathic cholestasis of pregnancy (ICP) was detected in 10 out of the 32 multiparous women in the last two generations of a large kindred. The affected women are distributed in five family units, sharing Chilean-born great-grandparents. The connection between the ICP-affected women and the common trunk is given both by male and female parents. Five cases are concentrated in one family unit, where the mother and all her daughters have been affected by the disease. The role of genetic factors in the pathogenesis of ICP is supported by this study. It is proposed that the disease may be transmitted as a predisposing trait by individuals of either sex and that non-genetic factors may influence its expressivity. A reliable test to identify all the genetic carriers seems indispensable to define the pattern of inheritance in this disease.", "contents": "Idiopathic cholestasis of pregnancy in a large kindred. A past history of idiopathic cholestasis of pregnancy (ICP) was detected in 10 out of the 32 multiparous women in the last two generations of a large kindred. The affected women are distributed in five family units, sharing Chilean-born great-grandparents. The connection between the ICP-affected women and the common trunk is given both by male and female parents. Five cases are concentrated in one family unit, where the mother and all her daughters have been affected by the disease. The role of genetic factors in the pathogenesis of ICP is supported by this study. It is proposed that the disease may be transmitted as a predisposing trait by individuals of either sex and that non-genetic factors may influence its expressivity. A reliable test to identify all the genetic carriers seems indispensable to define the pattern of inheritance in this disease.", "PMID": 976812}
{"id": "pubmed23n0032_10897", "title": "Gastritis duodenitis, and circulating levels of gastrin in duodenal ulcer before and after vagotomy.", "content": "Biopsy specimens have been taken from five standard sites in the stomach and from the duodenal bulb in order to investigate the association of gastritis and duodenitis with duodenal ulcer. Twenty patients with chronic duodenal ulcer were investigated in this manner and in addition had gastric secretion tests and a radio-immune assay of serum gastrin under differing conditions. The patients were then treated either by a truncal vagotomy and pyloroplasty (TVP) or by a highly selective vagotomy without a drainage procedure (HSV). All the investigations were repeated three months postoperatively. Duodenal ulcer was usually associated with gastriitis, although this varied in extent and severity from patient to patient. In nearly all the patients, gastritis was present at the pyloric end of the stomach and along the lesser curve. In more than half of the patients, gastritis was also present in the body of the stomach but the fundus was usually spared. Chronic duodenitis was found in the duodenal bulb in all these patients. After vagotomy there was a marked increase in both the extent and severity of the proximal gastritis in both treatment groups but the distal gastritis remain almost unchanged. There was little change in the incidence of duodenitis after vagotomy but its severity was lessened. No correlation was found between the peak acid output (PAO) in response to Histalog and the severity of the gastritis or the duodenitis either before or after operation, with one exception. The postoperative PAO was significantly less in those patients who developed a severe proximal gastritis after vagotomy. No relationship was found between the severity of the distal gastritis and the levels of serum gastrin. No correlation was found between either the basal or peak acid output and the corresponding serum gastrin levels before or after vagotomy.", "contents": "Gastritis duodenitis, and circulating levels of gastrin in duodenal ulcer before and after vagotomy. Biopsy specimens have been taken from five standard sites in the stomach and from the duodenal bulb in order to investigate the association of gastritis and duodenitis with duodenal ulcer. Twenty patients with chronic duodenal ulcer were investigated in this manner and in addition had gastric secretion tests and a radio-immune assay of serum gastrin under differing conditions. The patients were then treated either by a truncal vagotomy and pyloroplasty (TVP) or by a highly selective vagotomy without a drainage procedure (HSV). All the investigations were repeated three months postoperatively. Duodenal ulcer was usually associated with gastriitis, although this varied in extent and severity from patient to patient. In nearly all the patients, gastritis was present at the pyloric end of the stomach and along the lesser curve. In more than half of the patients, gastritis was also present in the body of the stomach but the fundus was usually spared. Chronic duodenitis was found in the duodenal bulb in all these patients. After vagotomy there was a marked increase in both the extent and severity of the proximal gastritis in both treatment groups but the distal gastritis remain almost unchanged. There was little change in the incidence of duodenitis after vagotomy but its severity was lessened. No correlation was found between the peak acid output (PAO) in response to Histalog and the severity of the gastritis or the duodenitis either before or after operation, with one exception. The postoperative PAO was significantly less in those patients who developed a severe proximal gastritis after vagotomy. No relationship was found between the severity of the distal gastritis and the levels of serum gastrin. No correlation was found between either the basal or peak acid output and the corresponding serum gastrin levels before or after vagotomy.", "PMID": 976813}
{"id": "pubmed23n0032_10898", "title": "Transient paraproteinaemia in a patient with coeliac disease.", "content": "A case is reported of a 43 year old man who suffered from a grass pollen allergy and a malabsorption syndrome and in whom a paraproteinaemia was found. The grass pollen hypersensitivity was abolished by desensitization. The malabsorption syndrome was found to be due to coeliac disease--that is, a \"flat\" mucosa of the jejunum with an almost normal ileal mucosa--followed by clinical recovery and morphological improvement on a gluten-free diet. A short period of gluten reintroduction caused deterioration of the jejunum. The monoclonal immunoglobulin (IgG-gamma) diminished and disappeared in the course of three years. Although it has not been possible to demonstrate that this paraprotein had anti-gliadin activity, it is suggested that the constant stimulation of the gut reticuloendothelial system by gluten might bear some relation to the appearance of the paraproteinaemia.", "contents": "Transient paraproteinaemia in a patient with coeliac disease. A case is reported of a 43 year old man who suffered from a grass pollen allergy and a malabsorption syndrome and in whom a paraproteinaemia was found. The grass pollen hypersensitivity was abolished by desensitization. The malabsorption syndrome was found to be due to coeliac disease--that is, a \"flat\" mucosa of the jejunum with an almost normal ileal mucosa--followed by clinical recovery and morphological improvement on a gluten-free diet. A short period of gluten reintroduction caused deterioration of the jejunum. The monoclonal immunoglobulin (IgG-gamma) diminished and disappeared in the course of three years. Although it has not been possible to demonstrate that this paraprotein had anti-gliadin activity, it is suggested that the constant stimulation of the gut reticuloendothelial system by gluten might bear some relation to the appearance of the paraproteinaemia.", "PMID": 976814}
{"id": "pubmed23n0032_10899", "title": "The superficialis minus deformity and its operative treatment.", "content": "On removal of the superficial finger flexor the normal sequence of flexion is greatly disturbed if the proximal interphalangeal joint is hypermobile, and a zig-zag deformity results. The pathomechanics of this problem as described by Landsmeer (1958) is reviewed. A modification of Littler's lateral band re-routing operation for correction of the intrinsic plus finger deformity has been developed to correct the superficialis minus deformity. This procedure has been successful in correcting the proximal interphalangeal joint hyperextension in five cases and in preventing its development in three others. Terminal interphalangeal joint flexion deformity has not been eliminated.", "contents": "The superficialis minus deformity and its operative treatment. On removal of the superficial finger flexor the normal sequence of flexion is greatly disturbed if the proximal interphalangeal joint is hypermobile, and a zig-zag deformity results. The pathomechanics of this problem as described by Landsmeer (1958) is reviewed. A modification of Littler's lateral band re-routing operation for correction of the intrinsic plus finger deformity has been developed to correct the superficialis minus deformity. This procedure has been successful in correcting the proximal interphalangeal joint hyperextension in five cases and in preventing its development in three others. Terminal interphalangeal joint flexion deformity has not been eliminated.", "PMID": 976817}
{"id": "pubmed23n0032_10900", "title": "Swan-neck deformity: mechanism and surgical treatment.", "content": "There are several forms of swan-neck deformity which are amenable to surgical treatment with good results, if based on an adequate study of the mechanism. Selection of the most suitable surgical procedure must be based on the pathological type.", "contents": "Swan-neck deformity: mechanism and surgical treatment. There are several forms of swan-neck deformity which are amenable to surgical treatment with good results, if based on an adequate study of the mechanism. Selection of the most suitable surgical procedure must be based on the pathological type.", "PMID": 976818}
{"id": "pubmed23n0032_10901", "title": "Surface study of tenosynovium in hens and humans by electron microscopy.", "content": "A comparative study has been made of the surface structure of the tenosynovium in flexor tendon apparatus obtained from hens and humans. Three different parts of the visceral, and parietal layers, and vincula were observed by scanning electron microscopy. Lining cells of the visceral layer are relatively flat and enmeshed with filamentous fibrils, while those of the parietal layer and the vincula are covered with vesicular and granular particles. Intercellular space is occupied with a close meshwork of filamentous fibrils. These findings are similar in both hens and humans. It is, thus, suggested that the cellular structure varies in the three different parts of the tenosynovium probably in relation to their function.", "contents": "Surface study of tenosynovium in hens and humans by electron microscopy. A comparative study has been made of the surface structure of the tenosynovium in flexor tendon apparatus obtained from hens and humans. Three different parts of the visceral, and parietal layers, and vincula were observed by scanning electron microscopy. Lining cells of the visceral layer are relatively flat and enmeshed with filamentous fibrils, while those of the parietal layer and the vincula are covered with vesicular and granular particles. Intercellular space is occupied with a close meshwork of filamentous fibrils. These findings are similar in both hens and humans. It is, thus, suggested that the cellular structure varies in the three different parts of the tenosynovium probably in relation to their function.", "PMID": 976819}
{"id": "pubmed23n0032_10902", "title": "Scanning electron microscopy of repaired tendon and pseudosheath.", "content": "(1) Repairing surface structure of transected digital flexor tendons of hens were studied by scanning electron microscopy. One week after a simple suture, fibrous materials probably of fibrin and exudated blood cells were attached on the surface. After two weeks, lining cells enmeshed with fine fibrils covered most of the surface. After three weeks, cellular protrusions of spindle shape spread over the repaired tendon. (2) Repaired surface of partially resected tendons of hens showed the same sort of healing processes as observed in the sutured tendons. The injured tendon surface was covered with the lining cells derived from surrounding synovium. (3) It was suggested that the repair of the injured tendons may occur from the surrounding tenosynovium, where the fibroplastic-like lining cells cover the injured part. (4) Observation of surface structure of pseudosheath was carried out after the silicone rod implantation with complete resection of the surrounding sheath. In one or two weeks, spindle-like cells probably corresponding to fibroblasts appeared on the surface. Fibrillar substance also mingled with such fibroblastic cell protrusions. Well-repaired pseudosheath similar to the normal parietal sheath was seen at four weeks. Such differentiated synovial lining cells formed from the peritendinous tissues were also regenerated from the original area around this time. (5) It could be said that the experimental repair on the visceral and parietal layer of the tendon sheath was similar in time and form. However, it seems to be necessary to compare the results from human subjects in relation to the abnormal repair of the injured tendon such as in adhesions.", "contents": "Scanning electron microscopy of repaired tendon and pseudosheath. (1) Repairing surface structure of transected digital flexor tendons of hens were studied by scanning electron microscopy. One week after a simple suture, fibrous materials probably of fibrin and exudated blood cells were attached on the surface. After two weeks, lining cells enmeshed with fine fibrils covered most of the surface. After three weeks, cellular protrusions of spindle shape spread over the repaired tendon. (2) Repaired surface of partially resected tendons of hens showed the same sort of healing processes as observed in the sutured tendons. The injured tendon surface was covered with the lining cells derived from surrounding synovium. (3) It was suggested that the repair of the injured tendons may occur from the surrounding tenosynovium, where the fibroplastic-like lining cells cover the injured part. (4) Observation of surface structure of pseudosheath was carried out after the silicone rod implantation with complete resection of the surrounding sheath. In one or two weeks, spindle-like cells probably corresponding to fibroblasts appeared on the surface. Fibrillar substance also mingled with such fibroblastic cell protrusions. Well-repaired pseudosheath similar to the normal parietal sheath was seen at four weeks. Such differentiated synovial lining cells formed from the peritendinous tissues were also regenerated from the original area around this time. (5) It could be said that the experimental repair on the visceral and parietal layer of the tendon sheath was similar in time and form. However, it seems to be necessary to compare the results from human subjects in relation to the abnormal repair of the injured tendon such as in adhesions.", "PMID": 976820}
{"id": "pubmed23n0032_10903", "title": "Experimental flexor tendon healing without adhesion formation--a new concept of tendon nutrition and intrinsic healing mechanisms. A preliminary report.", "content": "An experimental model is presented enabling an analysis of the healing process of completely cut and re-sutured free segments of rabbit flexor tendons, kept avascular in a synovial milieu and completely isolated from adhesion formation. Under these conditions the cut tendons heal within a few weeks. It can be shown that this healing process is a result of intrinsic tendon cell activity only.", "contents": "Experimental flexor tendon healing without adhesion formation--a new concept of tendon nutrition and intrinsic healing mechanisms. A preliminary report. An experimental model is presented enabling an analysis of the healing process of completely cut and re-sutured free segments of rabbit flexor tendons, kept avascular in a synovial milieu and completely isolated from adhesion formation. Under these conditions the cut tendons heal within a few weeks. It can be shown that this healing process is a result of intrinsic tendon cell activity only.", "PMID": 976821}
{"id": "pubmed23n0032_10904", "title": "The relationship of the ulnar nerve to the medial intermuscular septum in the arm and its clinical significance.", "content": "In the region of the passage of the ulnar nerve from the anterior to the posterior compartment of the arm an arcade is formed which can be a primary or secondary factor in the production of traumatic lesions of this nerve in the arm. It is the purpose of this paper to present the details of this arcade and to note its clinical significance.", "contents": "The relationship of the ulnar nerve to the medial intermuscular septum in the arm and its clinical significance. In the region of the passage of the ulnar nerve from the anterior to the posterior compartment of the arm an arcade is formed which can be a primary or secondary factor in the production of traumatic lesions of this nerve in the arm. It is the purpose of this paper to present the details of this arcade and to note its clinical significance.", "PMID": 976822}
{"id": "pubmed23n0032_10905", "title": "In search of complications in carpal tunnel decompression.", "content": "A series of 170 hands in 152 cases of carpal tunnel decompression presented from the Hand Unit of the Royal Victoria Infirmary, Newcastle-upon-Tyne. Special emphasis is laid on unsatisfactory results of the surgical procedure and their remedy.", "contents": "In search of complications in carpal tunnel decompression. A series of 170 hands in 152 cases of carpal tunnel decompression presented from the Hand Unit of the Royal Victoria Infirmary, Newcastle-upon-Tyne. Special emphasis is laid on unsatisfactory results of the surgical procedure and their remedy.", "PMID": 976823}
{"id": "pubmed23n0032_10906", "title": "Sensory re-education after median nerve lesions.", "content": "Technique for re-educating sensory function after median nerve lesions at the wrist is described. Results of re-education of Twenty-three patients are presented. The functional results are good and belie the traditional view of sensory function after nerve suture. Recent advances in sensory neuro-physiology are discussed which may explain the successes of this technique.", "contents": "Sensory re-education after median nerve lesions. Technique for re-educating sensory function after median nerve lesions at the wrist is described. Results of re-education of Twenty-three patients are presented. The functional results are good and belie the traditional view of sensory function after nerve suture. Recent advances in sensory neuro-physiology are discussed which may explain the successes of this technique.", "PMID": 976824}
{"id": "pubmed23n0032_10907", "title": "Survival of the thumb by a vein graft in a case of a severe hand lesion.", "content": "A case of severe lesion of the hand is reported in which the circulation of the thumb was restored by using a vein graft. The operative procedure and postoperative management are described. The periods of arterial insufficiency in the thumb, likely due to spasms, are discussed.", "contents": "Survival of the thumb by a vein graft in a case of a severe hand lesion. A case of severe lesion of the hand is reported in which the circulation of the thumb was restored by using a vein graft. The operative procedure and postoperative management are described. The periods of arterial insufficiency in the thumb, likely due to spasms, are discussed.", "PMID": 976826}
{"id": "pubmed23n0032_10908", "title": "Dupuytren's disease--a study of the pattern of distribution and stage of contracture in the hand.", "content": "The distribution and stages of contracture in the digits are examined in 901 persons with Dupuytren's disease and collected into an epidemiological study of 15,950 persons in a small Norwegian town. The frequency of contracted digits counted from the total number of affected hands, are for men (women in parentheses): Thumb 3.0% (0.6), Index finger 1.2% (1.7), Middle finger 28.3% (27.9), Ring finger 85.1% (92.3) and Little finger 45.4% (39.5). The most common combination of affected fingers are ring and little fingers. At corresponding ages, the contracture is more severe in men than in women, and more severe in the right than in the left hand. With the exception of the index finger, the degree of contracture increases from the thumb to the little finger, 29% of the men and 12% of the women examined had contracture of such an extent that surgical treatment was advisable. Only 5.7% of the men and 3.1% of the women had been operated on because of the disease, which indicates that operation statistics are based upon very selected patients. Many aspects of this disease can only be properly studied in epidemiological material.", "contents": "Dupuytren's disease--a study of the pattern of distribution and stage of contracture in the hand. The distribution and stages of contracture in the digits are examined in 901 persons with Dupuytren's disease and collected into an epidemiological study of 15,950 persons in a small Norwegian town. The frequency of contracted digits counted from the total number of affected hands, are for men (women in parentheses): Thumb 3.0% (0.6), Index finger 1.2% (1.7), Middle finger 28.3% (27.9), Ring finger 85.1% (92.3) and Little finger 45.4% (39.5). The most common combination of affected fingers are ring and little fingers. At corresponding ages, the contracture is more severe in men than in women, and more severe in the right than in the left hand. With the exception of the index finger, the degree of contracture increases from the thumb to the little finger, 29% of the men and 12% of the women examined had contracture of such an extent that surgical treatment was advisable. Only 5.7% of the men and 3.1% of the women had been operated on because of the disease, which indicates that operation statistics are based upon very selected patients. Many aspects of this disease can only be properly studied in epidemiological material.", "PMID": 976827}
{"id": "pubmed23n0032_10909", "title": "Open palm technique for Dupuytren's contracture.", "content": "A study of the pre-operative condition of seventy-five hands afflicted with Dupuytren's contracture is followed by an analysis of the results of limited fasciectomy approached by midlateral digital and transverse palmar excisions. The palmar wounds were left open and healing occurred with minimal complications. The correction of contractures compares favourably with that achieved using other techniques of access and wound closure.", "contents": "Open palm technique for Dupuytren's contracture. A study of the pre-operative condition of seventy-five hands afflicted with Dupuytren's contracture is followed by an analysis of the results of limited fasciectomy approached by midlateral digital and transverse palmar excisions. The palmar wounds were left open and healing occurred with minimal complications. The correction of contractures compares favourably with that achieved using other techniques of access and wound closure.", "PMID": 976828}
{"id": "pubmed23n0032_10910", "title": "Surgery for rupture of extensor tendons in rheumatoid arthritis.", "content": "Forty-two patients underwent fifty-four operations for rupture of extensor tendons in rheumatoid disease. The results are analysed and factors affecting the results discussed.", "contents": "Surgery for rupture of extensor tendons in rheumatoid arthritis. Forty-two patients underwent fifty-four operations for rupture of extensor tendons in rheumatoid disease. The results are analysed and factors affecting the results discussed.", "PMID": 976829}
{"id": "pubmed23n0032_10911", "title": "Palmaris longus, anteductor of the thumb.", "content": "Palmaris longus contributes to the anteposition and pronation of the thumb under circumstances. It is however restricted by its medial tendon which runs into the palmar aponeurosis. The diversion of this tendon and the mobilisation of the muscular head of the abductor pollicis brevis arising from its lateral tendon will substantially accentuate the r\u00f4le of palmaris longus as a muscle of the thumb.", "contents": "Palmaris longus, anteductor of the thumb. Palmaris longus contributes to the anteposition and pronation of the thumb under circumstances. It is however restricted by its medial tendon which runs into the palmar aponeurosis. The diversion of this tendon and the mobilisation of the muscular head of the abductor pollicis brevis arising from its lateral tendon will substantially accentuate the r\u00f4le of palmaris longus as a muscle of the thumb.", "PMID": 976830}
{"id": "pubmed23n0032_10912", "title": "Subcapital fractures of the four ulnar metacarpal bones.", "content": "Several methods of reduction and fixation of subcapital fractures of the four ulnar metacarpals have been suggested. All current methods deal with the volar angulation of the fracture; but in 133 consecutive cases angulation in this direction was of little clinical consequence. The more serious sequelae must be ascribed either to rotary deformity or to ulnar angulation at the fracture-site. These deformities were usually the result of bad bandaging technique and not due to the initial trauma. A new principle of conservative treatment is suggested.", "contents": "Subcapital fractures of the four ulnar metacarpal bones. Several methods of reduction and fixation of subcapital fractures of the four ulnar metacarpals have been suggested. All current methods deal with the volar angulation of the fracture; but in 133 consecutive cases angulation in this direction was of little clinical consequence. The more serious sequelae must be ascribed either to rotary deformity or to ulnar angulation at the fracture-site. These deformities were usually the result of bad bandaging technique and not due to the initial trauma. A new principle of conservative treatment is suggested.", "PMID": 976831}
{"id": "pubmed23n0032_10913", "title": "Congenital absence of flexor pollicis longus and flexor pollicis brevis: a case report.", "content": "A case of the absence of the flexor pollicis longus and flexor pollicis brevis alone, a congenital anomaly of exceedingly rare occurrence, is reported. Literature relevant to the absence of muscles and tendons of the hand is reviewed and discussed.", "contents": "Congenital absence of flexor pollicis longus and flexor pollicis brevis: a case report. A case of the absence of the flexor pollicis longus and flexor pollicis brevis alone, a congenital anomaly of exceedingly rare occurrence, is reported. Literature relevant to the absence of muscles and tendons of the hand is reviewed and discussed.", "PMID": 976832}
{"id": "pubmed23n0032_10914", "title": "Cleft palate patients--the social work approach.", "content": "From birth to maturity, the needs of cleft lip and cleft palate patients change. For such patients, fixed teams of specialists headed by a surgeon may not be the best answer. The authors outline theclinicl characteristics of both deformities and show how social workers are uniquely equipped to coordinate a changing constellation of helpers in an adaptable form of long-term treatment.", "contents": "Cleft palate patients--the social work approach. From birth to maturity, the needs of cleft lip and cleft palate patients change. For such patients, fixed teams of specialists headed by a surgeon may not be the best answer. The authors outline theclinicl characteristics of both deformities and show how social workers are uniquely equipped to coordinate a changing constellation of helpers in an adaptable form of long-term treatment.", "PMID": 976860}
{"id": "pubmed23n0032_10915", "title": "Psychiatric social workers in the emergency department.", "content": "The authors report on a unique program that uses psychiatric social workers as the primary mental health care-givers in the emergency department of a general hospital. Their article demonstrates that the social worker is uniquely well equipped to respond to the psychiatric and social problems of emergency department patients.", "contents": "Psychiatric social workers in the emergency department. The authors report on a unique program that uses psychiatric social workers as the primary mental health care-givers in the emergency department of a general hospital. Their article demonstrates that the social worker is uniquely well equipped to respond to the psychiatric and social problems of emergency department patients.", "PMID": 976866}
{"id": "pubmed23n0032_10916", "title": "Group work with patients on peritoneal dialysis.", "content": "The patient in end-stage renal failure, whose life depends on an artificial kidney machine, must make major emotional and physical adjustments. The author describes how in-hospital group treatment can help these patients cope with their new life situation.", "contents": "Group work with patients on peritoneal dialysis. The patient in end-stage renal failure, whose life depends on an artificial kidney machine, must make major emotional and physical adjustments. The author describes how in-hospital group treatment can help these patients cope with their new life situation.", "PMID": 976867}
{"id": "pubmed23n0032_10917", "title": "Outreach services in \"God's waiting room\".", "content": "Miami Beach--or \"God's Waiting Room\" as some have flippantly named it--has an overwhelming number of elderly people living on low incomes. This article describes the community-based programs instituted by one long-term care facility that not only provide a viable alternative to institutionalization, but are cost effective as well.", "contents": "Outreach services in \"God's waiting room\". Miami Beach--or \"God's Waiting Room\" as some have flippantly named it--has an overwhelming number of elderly people living on low incomes. This article describes the community-based programs instituted by one long-term care facility that not only provide a viable alternative to institutionalization, but are cost effective as well.", "PMID": 976871}
{"id": "pubmed23n0032_10918", "title": "Prospects for social workers in health planning.", "content": "The nature and extent of social worker's involvement in regional health planning agencies was the subject of the nationwide study reproted here. Social work proved to be more heavily represented on the professional staffs of these agencies than any other discipline. This was just one of the findings that can help shape the profession's response to the possibilities this new field represents.", "contents": "Prospects for social workers in health planning. The nature and extent of social worker's involvement in regional health planning agencies was the subject of the nationwide study reproted here. Social work proved to be more heavily represented on the professional staffs of these agencies than any other discipline. This was just one of the findings that can help shape the profession's response to the possibilities this new field represents.", "PMID": 976872}
{"id": "pubmed23n0032_10919", "title": "Predicting the impact of severe illness in families.", "content": "The goals of this survey of families with a leukemic child were (1) to develop simple, objective methods of describing the overwhelming impact of this serious illness on families, and (2) to test the value of family coping responses that manifested themselves immediately after the diagnosis in predicting stress outcome as measured a few months after the death of the child. The findings indicate that most of the families with a seriously ill child were still overwhelmed by this crisis three months postmortem. The effectiveness of these families appeared, at that time, to have been substantially reduced in that their members suffered from multiple problems affecting their health and normal functioning. The evidence indicates substantial differences in the impact of the illness on the survey families, in accord with the effectiveness of their initial coping response to the diagnosis. Adaptive coping families were far superior in outcome than were maladaptive coping families in the follow-up survey. The study findings support the view that the optimal period for intervention is during the early, acute stage of the crisis, immediately following the confirmation of the diagnosis, when the family is making those decisions associated with maladaptive coping and poor stress outcome.", "contents": "Predicting the impact of severe illness in families. The goals of this survey of families with a leukemic child were (1) to develop simple, objective methods of describing the overwhelming impact of this serious illness on families, and (2) to test the value of family coping responses that manifested themselves immediately after the diagnosis in predicting stress outcome as measured a few months after the death of the child. The findings indicate that most of the families with a seriously ill child were still overwhelmed by this crisis three months postmortem. The effectiveness of these families appeared, at that time, to have been substantially reduced in that their members suffered from multiple problems affecting their health and normal functioning. The evidence indicates substantial differences in the impact of the illness on the survey families, in accord with the effectiveness of their initial coping response to the diagnosis. Adaptive coping families were far superior in outcome than were maladaptive coping families in the follow-up survey. The study findings support the view that the optimal period for intervention is during the early, acute stage of the crisis, immediately following the confirmation of the diagnosis, when the family is making those decisions associated with maladaptive coping and poor stress outcome.", "PMID": 976873}
{"id": "pubmed23n0032_10920", "title": "Rape--a personal account.", "content": "At the time of the rape the author was a first-year student of social work. Her description of the experience and of the complex process of recovery makes evident the paucity of the social services that were available to her and the ways in which they could have helped.", "contents": "Rape--a personal account. At the time of the rape the author was a first-year student of social work. Her description of the experience and of the complex process of recovery makes evident the paucity of the social services that were available to her and the ways in which they could have helped.", "PMID": 976874}
{"id": "pubmed23n0032_10921", "title": "A therapeutic community for chronic mental patients.", "content": "When the number of state mental hospitals in California was sharply reduced, San Diego County organized a therapeutic community for the treatment of acting-out chronic mental patients. The program represents the constructive use of group pressure to instill adaptive behavior patterns and to imporve ego functioning.", "contents": "A therapeutic community for chronic mental patients. When the number of state mental hospitals in California was sharply reduced, San Diego County organized a therapeutic community for the treatment of acting-out chronic mental patients. The program represents the constructive use of group pressure to instill adaptive behavior patterns and to imporve ego functioning.", "PMID": 976875}
{"id": "pubmed23n0032_10922", "title": "Biological decomposition of fulvic acid preparations.", "content": "Decomposition of preparations of various fractions of fulvic acids in pure cultures of bacteria and in the soil was investigated. In the soils enriched with fulvic acids the amount of bacteria increased, oxygen consumption and formation of carbon dioxide followed a typical sigmoid curve. The above measurements indicated that mineralization occurred after a very short or negligible lag phase. During the decomposition of fulvic acids the ability of microorganisms to oxidize aromatic compounds, e.g. vanillic and p-hydroxybenzoic acid, increased. The presence of aromatic structures in the used preparations of fulvic acids was demonstrated on the basis of their IR spectra and according to the results of chromatographic analyses of their hydrolysates. The results indicated a relationship between metabolism of fulvic acids and aromatic compounds. In samples of the soil preincubated with glucose with fulvic acids decomposed more rapidly than in untreated samples.", "contents": "Biological decomposition of fulvic acid preparations. Decomposition of preparations of various fractions of fulvic acids in pure cultures of bacteria and in the soil was investigated. In the soils enriched with fulvic acids the amount of bacteria increased, oxygen consumption and formation of carbon dioxide followed a typical sigmoid curve. The above measurements indicated that mineralization occurred after a very short or negligible lag phase. During the decomposition of fulvic acids the ability of microorganisms to oxidize aromatic compounds, e.g. vanillic and p-hydroxybenzoic acid, increased. The presence of aromatic structures in the used preparations of fulvic acids was demonstrated on the basis of their IR spectra and according to the results of chromatographic analyses of their hydrolysates. The results indicated a relationship between metabolism of fulvic acids and aromatic compounds. In samples of the soil preincubated with glucose with fulvic acids decomposed more rapidly than in untreated samples.", "PMID": 976876}
{"id": "pubmed23n0032_10923", "title": "Colonial growth and ramihyphin A-induced changes in cell walls of Neurospora sitophila.", "content": "Colonial growth of Neurospora sitophila phenotypically induced by ramihyphin A is accompanied by marked changes in the contents of DNA, RNA and proteins in the mycelium, and in the relative proportion of hexoses in cell wall hydrolysates. The glucosamine/glucose ratio is also characteristic for colonial growth. X-ray analysis of cell walls showed that ramilhyphin A suppresses the crystalline arrangement of chitin in cell walls. A combination of microbiological, biochemical and physico-chemical methods yielded a general picture of the changes accompanying the colonial growth of Neurospora sitophila.", "contents": "Colonial growth and ramihyphin A-induced changes in cell walls of Neurospora sitophila. Colonial growth of Neurospora sitophila phenotypically induced by ramihyphin A is accompanied by marked changes in the contents of DNA, RNA and proteins in the mycelium, and in the relative proportion of hexoses in cell wall hydrolysates. The glucosamine/glucose ratio is also characteristic for colonial growth. X-ray analysis of cell walls showed that ramilhyphin A suppresses the crystalline arrangement of chitin in cell walls. A combination of microbiological, biochemical and physico-chemical methods yielded a general picture of the changes accompanying the colonial growth of Neurospora sitophila.", "PMID": 976877}
{"id": "pubmed23n0032_10924", "title": "Content of fatty acids of Chlorella kessleri in a deep tank fermentation.", "content": "Chlorella kessleri cultivated in a deep tank contained 4.8% of non-polar lipid; 51% of this fraction represents saturated fatty acids, 7% unsaturated fatty acids. Our investigation of the fatty acids profile demonstrated even- and odd-numbered saturated and unsaturated fatty acids ranging from C12 to C20. Unlike in other Chlorella species, stearic acid was the dominant fatty acid found. Also shown was an elevated C16:0 fatty acid content and a reduced level of unsaturated fatty acids.", "contents": "Content of fatty acids of Chlorella kessleri in a deep tank fermentation. Chlorella kessleri cultivated in a deep tank contained 4.8% of non-polar lipid; 51% of this fraction represents saturated fatty acids, 7% unsaturated fatty acids. Our investigation of the fatty acids profile demonstrated even- and odd-numbered saturated and unsaturated fatty acids ranging from C12 to C20. Unlike in other Chlorella species, stearic acid was the dominant fatty acid found. Also shown was an elevated C16:0 fatty acid content and a reduced level of unsaturated fatty acids.", "PMID": 976878}
{"id": "pubmed23n0032_10925", "title": "Oxidation of methanol, formaldehyde and formic acid by methanol-utilizing yeast.", "content": "Methanol-utilizing yeast, Candida boidini 11 Bh, characterized by high tolerance to methanol during growth, displays even higher tolerance when the oxidation rate by intact cells is tested. Low respiration activity is found even at 22% v/v of methanol. The half-saturation constant was 17-18 mM. The half-saturation constants for the two oxidation intermediates, formaldehyde and formic acid were 3.5-4.0 an .d 30-33 mM, respectively. When applied together with standard concentration of methanol, very low concentrations of both intermediates stimulated the oxidation rate. These results are discussed in connection with the relationship between growth and oxidation, the tolerance to high concentrations of inhibitory products and the mechanism of inhibition.", "contents": "Oxidation of methanol, formaldehyde and formic acid by methanol-utilizing yeast. Methanol-utilizing yeast, Candida boidini 11 Bh, characterized by high tolerance to methanol during growth, displays even higher tolerance when the oxidation rate by intact cells is tested. Low respiration activity is found even at 22% v/v of methanol. The half-saturation constant was 17-18 mM. The half-saturation constants for the two oxidation intermediates, formaldehyde and formic acid were 3.5-4.0 an .d 30-33 mM, respectively. When applied together with standard concentration of methanol, very low concentrations of both intermediates stimulated the oxidation rate. These results are discussed in connection with the relationship between growth and oxidation, the tolerance to high concentrations of inhibitory products and the mechanism of inhibition.", "PMID": 976879}
{"id": "pubmed23n0032_10926", "title": "Properties of the cured oncogenic strain 37400 of Agrobacterium tumefaciens.", "content": "The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured of prophages and can serve as a universal sensitive indicator for a number of phages isolated from various lysogenic strains of Agrobacterium tumefaciens. Its good growth properties in synthetic media and at elevated temperatues enable the isolation of auxotrophic mutants and temperature sensitive phage mutants. Preliminary experiments show that strain 37400 will serve as suitable starting material for conjugation experiments under defined conditions.", "contents": "Properties of the cured oncogenic strain 37400 of Agrobacterium tumefaciens. The properties of the 37400 oncogenic strain of Agrobacterium tumefaciens are described. This strain was derived from the VI lysogenic strain originally isolated by Hamilton from a Zinnia elegans tumour. Strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. It is cured of prophages and can serve as a universal sensitive indicator for a number of phages isolated from various lysogenic strains of Agrobacterium tumefaciens. Its good growth properties in synthetic media and at elevated temperatues enable the isolation of auxotrophic mutants and temperature sensitive phage mutants. Preliminary experiments show that strain 37400 will serve as suitable starting material for conjugation experiments under defined conditions.", "PMID": 976880}
{"id": "pubmed23n0032_10927", "title": "Application of mathematical optimization methods in microbiology.", "content": "Mathematical methods of experiment design have so far found little use in the optimization of microbiological processes. The conventional optimization procedure is still the transformation of functional relationship of n variables into n unidimensional optimizations; furthermore, the Box-Wilson gradient method is often used. This paper presents a review of methods used in other fields, and their application in microbiological practice. The methods are classified according to whether they require, besides the simple determination of the objective function (direct search methods), also the finding of its first (gradient methods) or second derivative (Newton-Raphson method). A modified Rosenbrock's method of direct optimum search and the gradient Box-Wilson method were used in parallel for the optimization of yeast growth on methanol. Their comparison showed that Rosenbrock's method is more suitbale for multiparameter systems.", "contents": "Application of mathematical optimization methods in microbiology. Mathematical methods of experiment design have so far found little use in the optimization of microbiological processes. The conventional optimization procedure is still the transformation of functional relationship of n variables into n unidimensional optimizations; furthermore, the Box-Wilson gradient method is often used. This paper presents a review of methods used in other fields, and their application in microbiological practice. The methods are classified according to whether they require, besides the simple determination of the objective function (direct search methods), also the finding of its first (gradient methods) or second derivative (Newton-Raphson method). A modified Rosenbrock's method of direct optimum search and the gradient Box-Wilson method were used in parallel for the optimization of yeast growth on methanol. Their comparison showed that Rosenbrock's method is more suitbale for multiparameter systems.", "PMID": 976882}
{"id": "pubmed23n0032_10928", "title": "A model to study cell injury: morphological changes of the proximal tubule of the rat kidney during ischemia and during recovery from ischemic injury in vivo.", "content": "Studies on structural cell changes as a function of ischemia time and studies on the recovery process of ischemically injured cells have implications for our general understanding of the features of cellular reaction to injury. For this reason a model was worked out to follow the morphological changes taking place in the proximal tubule of the rat kidney during ischemia and during recovery from an ischemic episode. The pars convoluta and the pars recta of the proximal tubule were examined separately and compared with one another.", "contents": "A model to study cell injury: morphological changes of the proximal tubule of the rat kidney during ischemia and during recovery from ischemic injury in vivo. Studies on structural cell changes as a function of ischemia time and studies on the recovery process of ischemically injured cells have implications for our general understanding of the features of cellular reaction to injury. For this reason a model was worked out to follow the morphological changes taking place in the proximal tubule of the rat kidney during ischemia and during recovery from an ischemic episode. The pars convoluta and the pars recta of the proximal tubule were examined separately and compared with one another.", "PMID": 976890}
{"id": "pubmed23n0032_10929", "title": "The recovery and identification of fluoroacetamide and fluoroacetic acid from tissues.", "content": "A rapid method is described for the extraction and identification of fluoroacetamide and fluoroacetic acid from tissues, by gas chromatography, using a mass spectrometer in the multiple ion monitoring mode as the detector. Recoveries from animal and human tissue macerates, after the addition of each compound, were approximately 30--35% for fluoroacetamide and 50--55% for fluoroacetic acid. Minimum detectable levels in tissues were found to be circa 0.7 mug/g for the amide and 0.1 mug/g for the acid. Visceral tissue levels were found to be approximately 3 mug/g for fluoroacetamide, and 2.5--10 mug/g fluoroacetic acid in guinea pigs and rabbits which had been fatally poisoned with these compounds.", "contents": "The recovery and identification of fluoroacetamide and fluoroacetic acid from tissues. A rapid method is described for the extraction and identification of fluoroacetamide and fluoroacetic acid from tissues, by gas chromatography, using a mass spectrometer in the multiple ion monitoring mode as the detector. Recoveries from animal and human tissue macerates, after the addition of each compound, were approximately 30--35% for fluoroacetamide and 50--55% for fluoroacetic acid. Minimum detectable levels in tissues were found to be circa 0.7 mug/g for the amide and 0.1 mug/g for the acid. Visceral tissue levels were found to be approximately 3 mug/g for fluoroacetamide, and 2.5--10 mug/g fluoroacetic acid in guinea pigs and rabbits which had been fatally poisoned with these compounds.", "PMID": 976898}
{"id": "pubmed23n0032_10930", "title": "Magnesium and other electrolytes in bovine eyeballs immersed in sea water and other fluids.", "content": "Bovine eyeballs were immersed in fluids including sea water for variable intervals of time, and magnesium concentrations and those of other electrolytes were determined in the aqueous and vitreous humor. Magnesium values increased with time, but no final saturation was reached nor were the levels obtained as high as those previously reported. In human immersion fatalities, Mg++ increased comparably to the most closely stimulating experimental situation, that is, corneal exposure in an unstirred medium. At best, vitreous Mg++ appears to be an imperfect marker in estimating the length of immersion, and then only if the sample remains unhemolyzed and the total post-mortem interval is considered. Establishing or excluding the diagnosis of drowning solely by this technique seems unlikely because of ion diffusion into the eye fluids.", "contents": "Magnesium and other electrolytes in bovine eyeballs immersed in sea water and other fluids. Bovine eyeballs were immersed in fluids including sea water for variable intervals of time, and magnesium concentrations and those of other electrolytes were determined in the aqueous and vitreous humor. Magnesium values increased with time, but no final saturation was reached nor were the levels obtained as high as those previously reported. In human immersion fatalities, Mg++ increased comparably to the most closely stimulating experimental situation, that is, corneal exposure in an unstirred medium. At best, vitreous Mg++ appears to be an imperfect marker in estimating the length of immersion, and then only if the sample remains unhemolyzed and the total post-mortem interval is considered. Establishing or excluding the diagnosis of drowning solely by this technique seems unlikely because of ion diffusion into the eye fluids.", "PMID": 976899}
{"id": "pubmed23n0032_10931", "title": "Antibody profiling of bloodstains.", "content": "The detection and measurement of antibodies in bloodstains represents a departure from the classical blood grouping used routinely in forensic serology. The antibodies in question are those associated with atopic allergy and infections by micro-organisms and other parasites. The production of these antibodies is not primarily under genetic control but reflects an individual's reaction to his environment. This paper describes the explicit information which may be obtained from an antibody profile, the high discriminating power and other advantages of the method.", "contents": "Antibody profiling of bloodstains. The detection and measurement of antibodies in bloodstains represents a departure from the classical blood grouping used routinely in forensic serology. The antibodies in question are those associated with atopic allergy and infections by micro-organisms and other parasites. The production of these antibodies is not primarily under genetic control but reflects an individual's reaction to his environment. This paper describes the explicit information which may be obtained from an antibody profile, the high discriminating power and other advantages of the method.", "PMID": 976900}
{"id": "pubmed23n0032_10932", "title": "Necropsy findings in fatal hypothermia cases.", "content": "A series of 22 cases of fatal accidental or suicidal hypothermia is presented. Necropsy findings on which the diagnosis can be based were analysed. Purple skin and swelling of face, hands and feet, as well as violet patches on elbows or knees were the most frequent external signs (Frequency 54--59%). The most conspicuous internal macroscopic signs were gastric erosions or haemorrhages, which were seen in half of the cases. Other less frequent signs were pulmonary oedema and acute renal and hepatic degeneration. Microscopically the myocardium showed small degenerative foci and/or fuchsinophilic fibres in two thirds of the cases. The myocardium was macroscopically normal. Histamine and serotonin assays from urine did not indicate increased excretion during exposure. Catecholamine concentrations in urine were high (greater than 0.1 mug/ml) in most hypothermia deaths indicating increased excretion due to cold. The best diagnostic signs seem to be purple skin and oedema in face and ears, stomach erosions, degenerative foci in myocardium and high concentration of catecholamines in the urine.", "contents": "Necropsy findings in fatal hypothermia cases. A series of 22 cases of fatal accidental or suicidal hypothermia is presented. Necropsy findings on which the diagnosis can be based were analysed. Purple skin and swelling of face, hands and feet, as well as violet patches on elbows or knees were the most frequent external signs (Frequency 54--59%). The most conspicuous internal macroscopic signs were gastric erosions or haemorrhages, which were seen in half of the cases. Other less frequent signs were pulmonary oedema and acute renal and hepatic degeneration. Microscopically the myocardium showed small degenerative foci and/or fuchsinophilic fibres in two thirds of the cases. The myocardium was macroscopically normal. Histamine and serotonin assays from urine did not indicate increased excretion during exposure. Catecholamine concentrations in urine were high (greater than 0.1 mug/ml) in most hypothermia deaths indicating increased excretion due to cold. The best diagnostic signs seem to be purple skin and oedema in face and ears, stomach erosions, degenerative foci in myocardium and high concentration of catecholamines in the urine.", "PMID": 976901}
{"id": "pubmed23n0032_10933", "title": "Determination of the sequence of writing on both sides of a paper.", "content": "One of the most difficult subjects the Examiner of Questioned Documents had to deal with is the determination of the sequence of written materials. As far as this author knows, techniques are available only for cases where the relevant writings are placed on the same side of the paper. Numerous experiments have been made on a range of papers varying in weight. In all of these tests both sides of the paper were typewritten as well as handwritten (pencil, pen, ball-point pen and fountain pen). Several samples are shown where the possibility of such determination is seen to be feasible, especially in cases where both sides are typewritten and where one is typewritten and the other is handwritten with pencil, ball-point pen and fountain pen.", "contents": "Determination of the sequence of writing on both sides of a paper. One of the most difficult subjects the Examiner of Questioned Documents had to deal with is the determination of the sequence of written materials. As far as this author knows, techniques are available only for cases where the relevant writings are placed on the same side of the paper. Numerous experiments have been made on a range of papers varying in weight. In all of these tests both sides of the paper were typewritten as well as handwritten (pencil, pen, ball-point pen and fountain pen). Several samples are shown where the possibility of such determination is seen to be feasible, especially in cases where both sides are typewritten and where one is typewritten and the other is handwritten with pencil, ball-point pen and fountain pen.", "PMID": 976902}
{"id": "pubmed23n0032_10934", "title": "[Dimensions of experience in encounter groups. Empirical studies on client-centered group activities].", "content": "The experiencing of participants of client-centered encounter groups served to demonstrate possibilities and limits of current models of the group process. This was done by analyzing the correlations between 5 dimensions of experiencing and aims of group participation, personality variables, modus of participation i.e. patient, student, therapist, time of participation, and group setting. The experiencing dimensions included 1. sympathy, trust, understanding, and help between members, 2. freedom from anxiety and stress, 3. openness, controversy and feedback, 4. momentary strong and basic feelings in one's self and others, 5. understanding, help and closeness of the group facilitator. The results show: Successful group participation was to the extent of maximal 50% determined by the experiences immanent in the client centered group process concept. Personality variables measured by tests determined the experiencing in the group to an extent of maximal 10%, supposedly less than the group experiencing was influenced by special characteristics of the group development for example the initial phase of group formation, member characteristics like age, attitudes concerning group work, and conventions of social behaviour. Even less relevant as determinants of group experiencing were differences of group setting and social pressure in the direction of uniform experiences at least within the first 20 hours of group participation.", "contents": "[Dimensions of experience in encounter groups. Empirical studies on client-centered group activities]. The experiencing of participants of client-centered encounter groups served to demonstrate possibilities and limits of current models of the group process. This was done by analyzing the correlations between 5 dimensions of experiencing and aims of group participation, personality variables, modus of participation i.e. patient, student, therapist, time of participation, and group setting. The experiencing dimensions included 1. sympathy, trust, understanding, and help between members, 2. freedom from anxiety and stress, 3. openness, controversy and feedback, 4. momentary strong and basic feelings in one's self and others, 5. understanding, help and closeness of the group facilitator. The results show: Successful group participation was to the extent of maximal 50% determined by the experiences immanent in the client centered group process concept. Personality variables measured by tests determined the experiencing in the group to an extent of maximal 10%, supposedly less than the group experiencing was influenced by special characteristics of the group development for example the initial phase of group formation, member characteristics like age, attitudes concerning group work, and conventions of social behaviour. Even less relevant as determinants of group experiencing were differences of group setting and social pressure in the direction of uniform experiences at least within the first 20 hours of group participation.", "PMID": 976916}
{"id": "pubmed23n0032_10935", "title": "[Chylothorax: causes, diagnosis, therapy].", "content": "The ductus thoracicus acts as the main vessel of the lymphatic system that flows into the venous system. It has the function of transporting lipis resorbed from the intestinal tract. Diseases of the thoracic duct are very rare and mostly result of injuries, tumors or as in most cases secondary infections. The main symptom in these cases is the presence of chylus outside of the ductus thoracicus, mainly in the thoracal spaces. Depending on the cause chylothorax is defined as traumatic, symptomatic or idiopathic. Therapy consists in drainage or ligature of the ductus thoracicus. The prognosis is good unless the cause is a malignant tumor resp. depends on the disease which is the cause of the chylothorax.", "contents": "[Chylothorax: causes, diagnosis, therapy]. The ductus thoracicus acts as the main vessel of the lymphatic system that flows into the venous system. It has the function of transporting lipis resorbed from the intestinal tract. Diseases of the thoracic duct are very rare and mostly result of injuries, tumors or as in most cases secondary infections. The main symptom in these cases is the presence of chylus outside of the ductus thoracicus, mainly in the thoracal spaces. Depending on the cause chylothorax is defined as traumatic, symptomatic or idiopathic. Therapy consists in drainage or ligature of the ductus thoracicus. The prognosis is good unless the cause is a malignant tumor resp. depends on the disease which is the cause of the chylothorax.", "PMID": 976917}
{"id": "pubmed23n0032_10936", "title": "[The so-called vinyl chloride disease in the Federal Republic of Germany].", "content": "A short outline is given of the historical development of the so-called vinyl chloride disease in the Federal Republic of Germany. It is a report on the measures taken--including the research work. Latest statistics about the cases of occupational disease are shown.", "contents": "[The so-called vinyl chloride disease in the Federal Republic of Germany]. A short outline is given of the historical development of the so-called vinyl chloride disease in the Federal Republic of Germany. It is a report on the measures taken--including the research work. Latest statistics about the cases of occupational disease are shown.", "PMID": 976918}
{"id": "pubmed23n0032_10937", "title": "[Why must we manufacture vinyl chloride and polyvinyl chloride?].", "content": "The manufacture of PVC is necessary for supplying the demands of modern society, for maintaining the equilibrium in the chemical industry such as the manufacture of caustic soda solution-chlorine by means of rocksalt electrolysis, and for the safe disposal of chlorine for environmental reasons. The manufacture of vinyl chloride and dichloroethane is illustrated. The conventional processing of PVC into consumer goods, after treatment of rigid and plasticized PVC compounds or granulate, is demonstrated.", "contents": "[Why must we manufacture vinyl chloride and polyvinyl chloride?]. The manufacture of PVC is necessary for supplying the demands of modern society, for maintaining the equilibrium in the chemical industry such as the manufacture of caustic soda solution-chlorine by means of rocksalt electrolysis, and for the safe disposal of chlorine for environmental reasons. The manufacture of vinyl chloride and dichloroethane is illustrated. The conventional processing of PVC into consumer goods, after treatment of rigid and plasticized PVC compounds or granulate, is demonstrated.", "PMID": 976919}
{"id": "pubmed23n0032_10938", "title": "[Serum uric acid and uric acid elimination after benzbromarone therapy in patients with gout and hyperuricemia].", "content": "During treatment with benzbromaron in a dose of 100 mg/day a significant fall of uric acid was shown beginning on the second day. On the forth day in the evening or the fifth day in the morning a steady state for the uric acid level is reached. The morning value was 2,9 mg/100 ml, the evening value 2,6 mg/100 ml. During treatment, even when serum uric acid levels are decreased, an augmented renal uric acid elimination excists but a hyperuraturia was not found.", "contents": "[Serum uric acid and uric acid elimination after benzbromarone therapy in patients with gout and hyperuricemia]. During treatment with benzbromaron in a dose of 100 mg/day a significant fall of uric acid was shown beginning on the second day. On the forth day in the evening or the fifth day in the morning a steady state for the uric acid level is reached. The morning value was 2,9 mg/100 ml, the evening value 2,6 mg/100 ml. During treatment, even when serum uric acid levels are decreased, an augmented renal uric acid elimination excists but a hyperuraturia was not found.", "PMID": 976920}
{"id": "pubmed23n0032_10939", "title": "[Myopathies. Possibilities of therapy].", "content": "There is still no useful form of therapy for muscle dystrophies. It is of importance not to miss the often difficult diagnosis of myositis, most inflammatory myopathies respond well to antiinflammatory therapy. Muscle disorders associated with endocrine diseases are curable by hormon replacement or correction of hormon overproduction. Myotonic muscle disease and hyper- or hypocalaemic muscle disease can be influenced positively by drug therapy.", "contents": "[Myopathies. Possibilities of therapy]. There is still no useful form of therapy for muscle dystrophies. It is of importance not to miss the often difficult diagnosis of myositis, most inflammatory myopathies respond well to antiinflammatory therapy. Muscle disorders associated with endocrine diseases are curable by hormon replacement or correction of hormon overproduction. Myotonic muscle disease and hyper- or hypocalaemic muscle disease can be influenced positively by drug therapy.", "PMID": 976922}
{"id": "pubmed23n0032_10940", "title": "[Acute side-effects of drugs. Causes and prevention].", "content": "A successful therapy with causative acting drugs (e.g. antibiotics, hormones) or with symptomatic acting drugs (e.g. antihypertensive, psychopharmacological drugs, anticoagulants) cannot be practicable without secondary effects. These unavoidable \"side-effects\" either are of no consequence or, these have to be risked and to be controlled, especially in long-term-therapy. Often, it is impossible to foresee allergic reactions. Avoidable \"side-effects\" may be dangerous in case of patient's self-medication, medical negligence or in case of drug interactions. 5 classes of drugs with possible threatening secondary effects are demonstrated: glykosides, sulfonylureas (antidiabetics), coumarin derivatives (anticoagulants), corticosteroids and parenteral contrast mediums.", "contents": "[Acute side-effects of drugs. Causes and prevention]. A successful therapy with causative acting drugs (e.g. antibiotics, hormones) or with symptomatic acting drugs (e.g. antihypertensive, psychopharmacological drugs, anticoagulants) cannot be practicable without secondary effects. These unavoidable \"side-effects\" either are of no consequence or, these have to be risked and to be controlled, especially in long-term-therapy. Often, it is impossible to foresee allergic reactions. Avoidable \"side-effects\" may be dangerous in case of patient's self-medication, medical negligence or in case of drug interactions. 5 classes of drugs with possible threatening secondary effects are demonstrated: glykosides, sulfonylureas (antidiabetics), coumarin derivatives (anticoagulants), corticosteroids and parenteral contrast mediums.", "PMID": 976925}
{"id": "pubmed23n0032_10941", "title": "[Fasting values of carbohydrate--and lipid metabolism in healthy subjects and in patients with diabetes mellitus].", "content": "In 106 healthy adults and 34 in-patients with diabetes mellitus the venous blood concentration of lactate, pyruvate, hydroxybutyrate and acetacetate was measured. In healthy men we found a lactate concentration of 7.7 mg/100 ml (7.11-8.15), a pyruvate level of 0.37 (0.34-0.44) mg/100 ml, a level of acetoacetate of 0.41 mg/100 ml(0.38-0.47) and a level of hydroxybutyrate of 0.47 mg/100 ml(0.29-0.70).", "contents": "[Fasting values of carbohydrate--and lipid metabolism in healthy subjects and in patients with diabetes mellitus]. In 106 healthy adults and 34 in-patients with diabetes mellitus the venous blood concentration of lactate, pyruvate, hydroxybutyrate and acetacetate was measured. In healthy men we found a lactate concentration of 7.7 mg/100 ml (7.11-8.15), a pyruvate level of 0.37 (0.34-0.44) mg/100 ml, a level of acetoacetate of 0.41 mg/100 ml(0.38-0.47) and a level of hydroxybutyrate of 0.47 mg/100 ml(0.29-0.70).", "PMID": 976926}
{"id": "pubmed23n0032_10942", "title": "[Muscular work in the therapy of diabetes mellitus].", "content": "A review is given on muscular exercise and its role in glucose homeostasis in normal and diabetic persons. Metabolic and hormonal consequences of short term and endurance exercise are described in detail. Based on clinical experience as well as on the basis of data published by the author dealing with animal experiments and clinical research studies the hypothesis of the \"permissive role\" of insulin with respect to the stimulatory effect of exercise on muscle glucose metabolism is put foreward and discussed. The metabolic effects of training in patients with diabetes mellitus are summarized. It is emphasized that in clinical research as well as in clinical routine muscular exercise as a means to treat metabolic disturbances has been widely neglected.", "contents": "[Muscular work in the therapy of diabetes mellitus]. A review is given on muscular exercise and its role in glucose homeostasis in normal and diabetic persons. Metabolic and hormonal consequences of short term and endurance exercise are described in detail. Based on clinical experience as well as on the basis of data published by the author dealing with animal experiments and clinical research studies the hypothesis of the \"permissive role\" of insulin with respect to the stimulatory effect of exercise on muscle glucose metabolism is put foreward and discussed. The metabolic effects of training in patients with diabetes mellitus are summarized. It is emphasized that in clinical research as well as in clinical routine muscular exercise as a means to treat metabolic disturbances has been widely neglected.", "PMID": 976928}
{"id": "pubmed23n0032_10943", "title": "[Malignant struma. Pathological anatomy and functional properties. Discussion comments on the contribution of J. Thurner and G. Montano].", "content": "Many different classifications of thyroid tumors were employed during the last 50 years. In 1964 the WHO International Reference Centre for the Histological Classification of Thyroid Tumors was established, in 1974 the final version of \"Histological Typing of Thyroid Tumours\" was published by Chr. Hedinger in collaboration with L. H. Sobin and 11 pathologists in 9 countries (\"WHO International Histological Classifications of Tumours No. 11\").", "contents": "[Malignant struma. Pathological anatomy and functional properties. Discussion comments on the contribution of J. Thurner and G. Montano]. Many different classifications of thyroid tumors were employed during the last 50 years. In 1964 the WHO International Reference Centre for the Histological Classification of Thyroid Tumors was established, in 1974 the final version of \"Histological Typing of Thyroid Tumours\" was published by Chr. Hedinger in collaboration with L. H. Sobin and 11 pathologists in 9 countries (\"WHO International Histological Classifications of Tumours No. 11\").", "PMID": 976930}
{"id": "pubmed23n0032_10944", "title": "Studies on the pituitary \"fettstoffwechselhormon\". VII. Analytical studies on two lipolytically high active peptides from hog pituitary glands.", "content": "First results of analytical studies on the highly purified lipolytic active hog pituitary fractions P-LF II C and P-LF II D are reported. Electrophoresis and end-group determinations indicate homogeneity for both peptides. The molecular weights, amino acid compositions, and the N- and C-terminal sequences of these peptides have been established and are compared with the corresponding results of other laboratories. The relation between biological activity and the structure of lipolytic active peptides is discussed.", "contents": "Studies on the pituitary \"fettstoffwechselhormon\". VII. Analytical studies on two lipolytically high active peptides from hog pituitary glands. First results of analytical studies on the highly purified lipolytic active hog pituitary fractions P-LF II C and P-LF II D are reported. Electrophoresis and end-group determinations indicate homogeneity for both peptides. The molecular weights, amino acid compositions, and the N- and C-terminal sequences of these peptides have been established and are compared with the corresponding results of other laboratories. The relation between biological activity and the structure of lipolytic active peptides is discussed.", "PMID": 976933}
{"id": "pubmed23n0032_10945", "title": "The contribution of the pancreas and the intestine to the regulation of lipolysis in birds. 2. Impaired lipolytic activity of pancreatic glucagon in the absence of either the pancreas or the intestine in the chicken.", "content": "Adipose tissue was removed from five-day old chickens after a) an overnight fast, b) total pancreatectomy and c) partial evisceration and digested with collagenase. The adipocytes were incubated with pancreatic glucagon (Novo) and the glycerol released into the medium taken as the index of lipolytic activity. A moderate fast, while being without effect on basal lipolysis, slightly decreased adipocyte sensitivity to glucagon. Pancreatectomy and evisceration significantly reduced both basal lipolysis and the response to glucagon: the impairment was most evident just at the concentration found in the plasma of such operated animals. It seems clear that normal lipolysis is under the control not only of pancreatic factors, but also of those of intestinal origin, and can only proceed normally when both are present.", "contents": "The contribution of the pancreas and the intestine to the regulation of lipolysis in birds. 2. Impaired lipolytic activity of pancreatic glucagon in the absence of either the pancreas or the intestine in the chicken. Adipose tissue was removed from five-day old chickens after a) an overnight fast, b) total pancreatectomy and c) partial evisceration and digested with collagenase. The adipocytes were incubated with pancreatic glucagon (Novo) and the glycerol released into the medium taken as the index of lipolytic activity. A moderate fast, while being without effect on basal lipolysis, slightly decreased adipocyte sensitivity to glucagon. Pancreatectomy and evisceration significantly reduced both basal lipolysis and the response to glucagon: the impairment was most evident just at the concentration found in the plasma of such operated animals. It seems clear that normal lipolysis is under the control not only of pancreatic factors, but also of those of intestinal origin, and can only proceed normally when both are present.", "PMID": 976934}
{"id": "pubmed23n0032_10946", "title": "Purification and characterization of a protein from porcine gut with glucagon-like immunoreactivity.", "content": "A protein with glucagon-like immunoreactivity has been isolated from porcine intestine in a highly purified form. The isoelectric point is 6.8-6.9, and the molecular weight is 11,625, as calculated from its amino acid composition: this estimate has been confirmed by S.D.S. gel electrophoresis. The partial sequence so far elucidated is from the N-terminal: Arg-Ser-Leu-Gin-Asn-Thr-Glx-Glx-Lys-Ala-Arg-Ser-Phe-, and from the C-terminal: -Ile-Ala, both differing from those of porcine pancreatic glucagon. On a molar basis the protein has the same immunoreactivity as porcine glucagon when assayed with some anti-glucagon sera, while the activity is less than 0.2% using other anti-glucagon sera.", "contents": "Purification and characterization of a protein from porcine gut with glucagon-like immunoreactivity. A protein with glucagon-like immunoreactivity has been isolated from porcine intestine in a highly purified form. The isoelectric point is 6.8-6.9, and the molecular weight is 11,625, as calculated from its amino acid composition: this estimate has been confirmed by S.D.S. gel electrophoresis. The partial sequence so far elucidated is from the N-terminal: Arg-Ser-Leu-Gin-Asn-Thr-Glx-Glx-Lys-Ala-Arg-Ser-Phe-, and from the C-terminal: -Ile-Ala, both differing from those of porcine pancreatic glucagon. On a molar basis the protein has the same immunoreactivity as porcine glucagon when assayed with some anti-glucagon sera, while the activity is less than 0.2% using other anti-glucagon sera.", "PMID": 976935}
{"id": "pubmed23n0032_10947", "title": "Effect of the fatty acid composition of ingested fats on rat liver intermediary metabolism.", "content": "The influence of a single load of medium chain triglycerides (MCT) or long chain triglycerides (LCT) on different intermediary metabolites of the rat liver was studied. Resultes showed that acetyl-CoA increased more after MCT than after LCT. MCT are more ketogenic than LCT. Mitochondrial and cytoplasmic redox state was greatly reduced after MCT. It is particularly interesting to note that MCT produced an increase in malate and citrate levels, whereas LCT had only a minor influence on these substances.", "contents": "Effect of the fatty acid composition of ingested fats on rat liver intermediary metabolism. The influence of a single load of medium chain triglycerides (MCT) or long chain triglycerides (LCT) on different intermediary metabolites of the rat liver was studied. Resultes showed that acetyl-CoA increased more after MCT than after LCT. MCT are more ketogenic than LCT. Mitochondrial and cytoplasmic redox state was greatly reduced after MCT. It is particularly interesting to note that MCT produced an increase in malate and citrate levels, whereas LCT had only a minor influence on these substances.", "PMID": 976936}
{"id": "pubmed23n0032_10948", "title": "Adrenal and liver participation in the rat's post-stress diabetic response.", "content": "Sixty minutes of restraint stress, preceded by chlorpromazine administration which stimulates somatotrophic hormone secretion (STH), produced an acute post-stress diabetic response (PDR) in normal-intact rats as well as in adrenalectomized rats. This PDR lasted 3 to 4 hours and was evaluated by glucemia and glucosuria determination and by the appearance of an insulin antagonist, alpha2-glycoprotein STH-dependent, called alpha2-inhibitor, which inhibits glucose uptake by isolated tissues. When tested in the suprahepatic blood of animals after stress it showed increased activity, both in normal and in adrenalectomized rats. This result permits us to state that alpha2-inhibitor may be produced in the liver by and action of STH and without primary glucocorticoid participation. The post-stress hyperglucemic response of adrenalectomized rats had a similar tendency to that of the control, although with initial and final values of glucemia significantly below the control. This response supports the diea that postadrenalectomy gluconeogenesis was evoked during and after the systemic stress.", "contents": "Adrenal and liver participation in the rat's post-stress diabetic response. Sixty minutes of restraint stress, preceded by chlorpromazine administration which stimulates somatotrophic hormone secretion (STH), produced an acute post-stress diabetic response (PDR) in normal-intact rats as well as in adrenalectomized rats. This PDR lasted 3 to 4 hours and was evaluated by glucemia and glucosuria determination and by the appearance of an insulin antagonist, alpha2-glycoprotein STH-dependent, called alpha2-inhibitor, which inhibits glucose uptake by isolated tissues. When tested in the suprahepatic blood of animals after stress it showed increased activity, both in normal and in adrenalectomized rats. This result permits us to state that alpha2-inhibitor may be produced in the liver by and action of STH and without primary glucocorticoid participation. The post-stress hyperglucemic response of adrenalectomized rats had a similar tendency to that of the control, although with initial and final values of glucemia significantly below the control. This response supports the diea that postadrenalectomy gluconeogenesis was evoked during and after the systemic stress.", "PMID": 976937}
{"id": "pubmed23n0032_10949", "title": "Diphenylhydantoin inhibition of pentagastrin-stimulated calcitonin secretion in the pig.", "content": "The present study examined the influence of diphenylhydantoin (DPH) on pentagastrin-induced calcitonin (CT) secretion in the pig. Diphenylhydantoin was observed to slow the onset and prolong the duration of pentagastrin stimulation on CT secretion. Further, pentagastrin reduced plasma calcium in both the control and DPH treated groups. The results of this study indicate a significant DPH-induced inhibition of pentagastrin-stimulated CT secretion in the pig.", "contents": "Diphenylhydantoin inhibition of pentagastrin-stimulated calcitonin secretion in the pig. The present study examined the influence of diphenylhydantoin (DPH) on pentagastrin-induced calcitonin (CT) secretion in the pig. Diphenylhydantoin was observed to slow the onset and prolong the duration of pentagastrin stimulation on CT secretion. Further, pentagastrin reduced plasma calcium in both the control and DPH treated groups. The results of this study indicate a significant DPH-induced inhibition of pentagastrin-stimulated CT secretion in the pig.", "PMID": 976938}
{"id": "pubmed23n0032_10950", "title": "Transport kinetics for the uptake of (3H)glycine and (3H)L-glutamate into dorsal and ventral slices of rat spinal cord.", "content": "The uptake of [3H]glycine and [3H]L-glutamate into slices of the dorsal and ventral halves of rat spinal cord taken from the thoracal (T3-9) and lumbar (L1-6) regions was determined in the concentration range 2 - 1 000muM. For both amino acids and in all four tissue pieces (thoracal dorsal and ventral, lumbar dorsal and ventral) biphasic linear regressions were obtained from the Lineweaver-Burk plots, demonstrating saturable transport mechanisms both in the high-affinity and in the low-affinity range. No significant dorsal-ventral or lumbar-thoracal differences were found for the transport constants Km of both amino acids. For the maximum velocity V similar dorsal-ventral and lumbar-thoracal gradients were found for both glycine and L-glutamate. These results, which are not entirely in agreement with the distribution of the endogenous pools of these amino acids, are critically discussed in view of the proposed function of high affinity uptake at the synapse.", "contents": "Transport kinetics for the uptake of (3H)glycine and (3H)L-glutamate into dorsal and ventral slices of rat spinal cord. The uptake of [3H]glycine and [3H]L-glutamate into slices of the dorsal and ventral halves of rat spinal cord taken from the thoracal (T3-9) and lumbar (L1-6) regions was determined in the concentration range 2 - 1 000muM. For both amino acids and in all four tissue pieces (thoracal dorsal and ventral, lumbar dorsal and ventral) biphasic linear regressions were obtained from the Lineweaver-Burk plots, demonstrating saturable transport mechanisms both in the high-affinity and in the low-affinity range. No significant dorsal-ventral or lumbar-thoracal differences were found for the transport constants Km of both amino acids. For the maximum velocity V similar dorsal-ventral and lumbar-thoracal gradients were found for both glycine and L-glutamate. These results, which are not entirely in agreement with the distribution of the endogenous pools of these amino acids, are critically discussed in view of the proposed function of high affinity uptake at the synapse.", "PMID": 976941}
{"id": "pubmed23n0032_10951", "title": "Chloramphenicol-induced loss of mitochondrial DNA polymerase activity in HeLa cells.", "content": "HeLa cells exposed to chloramphenicol for approximately one cell generation were found to contain a mitochondria-associated DNA polymerase with a significantly lower specific activity than that of control cells. This observation was not due to the presence of inhibitors in mitochondrial DNA polymerase preparations of chloramphenicol-treated cell cultures. In addition, there was no accumulation of a typical mitochondrial DNA polymerase in the post-mitochondrial supernatant of drug-treated cells.", "contents": "Chloramphenicol-induced loss of mitochondrial DNA polymerase activity in HeLa cells. HeLa cells exposed to chloramphenicol for approximately one cell generation were found to contain a mitochondria-associated DNA polymerase with a significantly lower specific activity than that of control cells. This observation was not due to the presence of inhibitors in mitochondrial DNA polymerase preparations of chloramphenicol-treated cell cultures. In addition, there was no accumulation of a typical mitochondrial DNA polymerase in the post-mitochondrial supernatant of drug-treated cells.", "PMID": 976942}
{"id": "pubmed23n0032_10952", "title": "Protection of actin from heat denaturation by various phallotoxins.", "content": "Phallotoxins protect F-actin from heat denaturation at a temperature of 70 degrees C for 3 min. This has been shown by difference spectroscopy. G-actin is not protected from heat denaturation by phalloidin. Among the various phallopeptides investigated, 4 toxic ones showed the same protecting ability, whereas non-toxic seco-compounds has no effect. The non-toxic (S)-phalloidinsulfoxide A exhibited only a partial protecting activity, corresponding to an affinity for F-actin of about one tenth of that of the toxic peptides. Evidence for this was obtained from the easy displacement of the sulfoxide A from F-actin by phalloidin as well as by a spectroscopic dilution titration.", "contents": "Protection of actin from heat denaturation by various phallotoxins. Phallotoxins protect F-actin from heat denaturation at a temperature of 70 degrees C for 3 min. This has been shown by difference spectroscopy. G-actin is not protected from heat denaturation by phalloidin. Among the various phallopeptides investigated, 4 toxic ones showed the same protecting ability, whereas non-toxic seco-compounds has no effect. The non-toxic (S)-phalloidinsulfoxide A exhibited only a partial protecting activity, corresponding to an affinity for F-actin of about one tenth of that of the toxic peptides. Evidence for this was obtained from the easy displacement of the sulfoxide A from F-actin by phalloidin as well as by a spectroscopic dilution titration.", "PMID": 976943}
{"id": "pubmed23n0032_10953", "title": "[Stimulation of RNA synthesis in rat liver and isolated hepatocytes by silybin, an antihepatotoxic agent from Silybum marianum L. Gaertn (author's transl)].", "content": "The incorporation of [3H] orotic acid into RNA from rat livers is stimulated by the flavonolignane derivative Silybin. The time course of the RNA synthesis and its dose dependence were demonstrated with isolated hepatocytes and [3H]uridine. The specific radioactivity of the newly synthesized RNA in rats treated with silybin is markedly higher than that of the controls. From preliminary experiments there is no indication that the synthesis of a distinct species of RNA is stimulated preferentially.", "contents": "[Stimulation of RNA synthesis in rat liver and isolated hepatocytes by silybin, an antihepatotoxic agent from Silybum marianum L. Gaertn (author's transl)]. The incorporation of [3H] orotic acid into RNA from rat livers is stimulated by the flavonolignane derivative Silybin. The time course of the RNA synthesis and its dose dependence were demonstrated with isolated hepatocytes and [3H]uridine. The specific radioactivity of the newly synthesized RNA in rats treated with silybin is markedly higher than that of the controls. From preliminary experiments there is no indication that the synthesis of a distinct species of RNA is stimulated preferentially.", "PMID": 976944}
{"id": "pubmed23n0032_10954", "title": "[Nepsilon-(beta-Aspartyl)lysine and Nepsilon-(gamma-glutamyl)lysine crosslinkings in bovine colostrum (author's transl)].", "content": "In bovine colostrum which was defatted and totally hydrolyzed by proteolytic enzymes, the isopeptides Asp Lys and Glu Lys were identified by ion-exchange chromatography and preparative separation. Isopeptides were identified in two of seven samples of colostrum. It is still to be clarified whether there is a correlation between the amound of gamma-glutamyltransferase and the appearance of these crosslinkings.", "contents": "[Nepsilon-(beta-Aspartyl)lysine and Nepsilon-(gamma-glutamyl)lysine crosslinkings in bovine colostrum (author's transl)]. In bovine colostrum which was defatted and totally hydrolyzed by proteolytic enzymes, the isopeptides Asp Lys and Glu Lys were identified by ion-exchange chromatography and preparative separation. Isopeptides were identified in two of seven samples of colostrum. It is still to be clarified whether there is a correlation between the amound of gamma-glutamyltransferase and the appearance of these crosslinkings.", "PMID": 976945}
{"id": "pubmed23n0032_10955", "title": "Kremens v. Bartley: the case for the state.", "content": "The authors, co-counsel for the state of Pennsylvania in the child-commitment case to be heard this fall by the Supreme Court, outline the background of the case and present the state's arguments for preserving the rights of parents to commit their children to mental institutions. They warn of the potential psychological harm to both family and child in pitting one against the other in a formal commitment hearing. They also content that the lower-court ruling that granted due-process rights to children causes more problems than it solves. The decision, for example, effectively abolishes the state's program of respite care for mentally retarded youths.", "contents": "Kremens v. Bartley: the case for the state. The authors, co-counsel for the state of Pennsylvania in the child-commitment case to be heard this fall by the Supreme Court, outline the background of the case and present the state's arguments for preserving the rights of parents to commit their children to mental institutions. They warn of the potential psychological harm to both family and child in pitting one against the other in a formal commitment hearing. They also content that the lower-court ruling that granted due-process rights to children causes more problems than it solves. The decision, for example, effectively abolishes the state's program of respite care for mentally retarded youths.", "PMID": 976952}
{"id": "pubmed23n0032_10956", "title": "Kremens v. Bartley: the right to be free.", "content": "The author, counsel for the children in the child-commitment case now before the Supreme Court, describes the necessity for legal protections for children prior to mental institutionalization. He maintains that frequently a child is placed in an institution not because he or she will benefit from such placement, but because it benefits his parents or eases family conflicts. He describes the dangers of institutionalization and notes the expansion of this mode of treatment for juveniles. He explains the events that led to the decision to argue for the rights of his clients to challenge their commitments, and he presents the legal arguments for granting children the same rights to due process as adults presently have.", "contents": "Kremens v. Bartley: the right to be free. The author, counsel for the children in the child-commitment case now before the Supreme Court, describes the necessity for legal protections for children prior to mental institutionalization. He maintains that frequently a child is placed in an institution not because he or she will benefit from such placement, but because it benefits his parents or eases family conflicts. He describes the dangers of institutionalization and notes the expansion of this mode of treatment for juveniles. He explains the events that led to the decision to argue for the rights of his clients to challenge their commitments, and he presents the legal arguments for granting children the same rights to due process as adults presently have.", "PMID": 976953}
{"id": "pubmed23n0032_10957", "title": "Mental health centers and the criminal justice system.", "content": "Through questionnaires, interviews, and site visits, the authors undertook to ascertain to what extent the 26 community mental health centers in Kansas were contributing to the resolution of problems that concern the criminal justice system. They found that in all large communities some reciprocal programs have developed between the two systems, but meaningful collaboration is rare in small communities. Juvenile courts, urban law enforcement agencies, and county probation officers are most receptive to collaborative programs. An evaluation of several effective programs revealed three basic conditions that attribute to their success: an urban community setting, individual initiative by staff from each system, and location of the program within the criminal justice system.", "contents": "Mental health centers and the criminal justice system. Through questionnaires, interviews, and site visits, the authors undertook to ascertain to what extent the 26 community mental health centers in Kansas were contributing to the resolution of problems that concern the criminal justice system. They found that in all large communities some reciprocal programs have developed between the two systems, but meaningful collaboration is rare in small communities. Juvenile courts, urban law enforcement agencies, and county probation officers are most receptive to collaborative programs. An evaluation of several effective programs revealed three basic conditions that attribute to their success: an urban community setting, individual initiative by staff from each system, and location of the program within the criminal justice system.", "PMID": 976954}
{"id": "pubmed23n0032_10958", "title": "A program for high school students in a facility for the retarded.", "content": "In 1971 the Fountain Valley (Calif.) High School established a program at Fairview State Hospital in Costa Mesa. California, in which 28 students worked with blind mentally retarded residents. There are now more than 160 students working in the program each semester in nine of the hospital's ten treatment programs; the students are a valuable source of one-to-one assistance. They increase the amount of personal care and attention that each resident receives, and they free staff members for other activities.", "contents": "A program for high school students in a facility for the retarded. In 1971 the Fountain Valley (Calif.) High School established a program at Fairview State Hospital in Costa Mesa. California, in which 28 students worked with blind mentally retarded residents. There are now more than 160 students working in the program each semester in nine of the hospital's ten treatment programs; the students are a valuable source of one-to-one assistance. They increase the amount of personal care and attention that each resident receives, and they free staff members for other activities.", "PMID": 976955}
{"id": "pubmed23n0032_10959", "title": "A program for the prevention and management of disturbed behavior.", "content": "A hospitalwide program to assist staff with the prevention and management of disturbed behavior of patients has been in operation at St. Thomas Psychiatric Hospital since January 1974. The staff development department prepared a teaching package consisting of a videotape cassette and a workbook that describes ways to predict and prevent disturbed behavior and to deal with it physically if it occurs. A comparison of hospital statistics for 1974 and 1975 indicates that in 1975 there was a decrease in the incidence of disturbed behavior, of patient injuries, and of staff injuries directly related to patients, and in the total man-hours lost.", "contents": "A program for the prevention and management of disturbed behavior. A hospitalwide program to assist staff with the prevention and management of disturbed behavior of patients has been in operation at St. Thomas Psychiatric Hospital since January 1974. The staff development department prepared a teaching package consisting of a videotape cassette and a workbook that describes ways to predict and prevent disturbed behavior and to deal with it physically if it occurs. A comparison of hospital statistics for 1974 and 1975 indicates that in 1975 there was a decrease in the incidence of disturbed behavior, of patient injuries, and of staff injuries directly related to patients, and in the total man-hours lost.", "PMID": 976956}
{"id": "pubmed23n0032_10960", "title": "Rural areas pose special problems for providing social services.", "content": "For maximum effectiveness, the delivery of social services and mental health services in a rural area must accommodate certain constraints imposed by the structure of the community. Accommodation to the community can be achieved best when an established and accepted institution provides structure and legitimization for the social service and the mental health worker and when it serves as a vehicle through which services are initiated and expanded.", "contents": "Rural areas pose special problems for providing social services. For maximum effectiveness, the delivery of social services and mental health services in a rural area must accommodate certain constraints imposed by the structure of the community. Accommodation to the community can be achieved best when an established and accepted institution provides structure and legitimization for the social service and the mental health worker and when it serves as a vehicle through which services are initiated and expanded.", "PMID": 976964}
{"id": "pubmed23n0032_10961", "title": "Activity of glucose-6-phosphate dehydrogenase among Indians living in a malarial region of Mato Grosso and its implication to the Indian-mixed populations in Brazil.", "content": "Erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity and electrophoresis were investigated among 154 Indians living in a region hyperendemic for malaria at Alto Xingu, Mato Grosso, Brazil. No enzyme-deficient individual was found, and all subjects belonged to enzyme type B. No statistical difference in G-6-PD levels was found between tribes and sexes. The average of G-6-PD activity of the Indians was significantly higher than the normal mean values found by the same technique in Caucasians, Negroes and Japanese of S\u00e4o Paulo, Brazil. The high rates of G-6-PD activity of the Indians are not correlated to an increased reticulocytosis by hypochromic anaemia and appear to be typical of Indian or Indian-mixed populations. Resistance to malaria in those populations should not involve erythrocyte G-6-PD deficiency. It is suggested that the apparent association between G-6-PD deficiency and resistance to malaria found in other populations could be a statistical accident determined by the racial correlation between the incidence of G-6-PD deficiency and sickle-cell or thalassaemic haemoglobins among Negro and Mediterranean populations.", "contents": "Activity of glucose-6-phosphate dehydrogenase among Indians living in a malarial region of Mato Grosso and its implication to the Indian-mixed populations in Brazil. Erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity and electrophoresis were investigated among 154 Indians living in a region hyperendemic for malaria at Alto Xingu, Mato Grosso, Brazil. No enzyme-deficient individual was found, and all subjects belonged to enzyme type B. No statistical difference in G-6-PD levels was found between tribes and sexes. The average of G-6-PD activity of the Indians was significantly higher than the normal mean values found by the same technique in Caucasians, Negroes and Japanese of S\u00e4o Paulo, Brazil. The high rates of G-6-PD activity of the Indians are not correlated to an increased reticulocytosis by hypochromic anaemia and appear to be typical of Indian or Indian-mixed populations. Resistance to malaria in those populations should not involve erythrocyte G-6-PD deficiency. It is suggested that the apparent association between G-6-PD deficiency and resistance to malaria found in other populations could be a statistical accident determined by the racial correlation between the incidence of G-6-PD deficiency and sickle-cell or thalassaemic haemoglobins among Negro and Mediterranean populations.", "PMID": 976992}
{"id": "pubmed23n0032_10962", "title": "Serum and red cell enzyme variants in an Amerindian tribe: the Sirionos (Eastern Bolivia).", "content": "Blood samples from 109 Siriono (Eastern Bolivia) belonging to the Tupi-Guarani group were investigated for enzyme variants in the following systems: glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, adenylate kinase, phospho-glucomutase (locus 1 and 2), acid phosphatases, lactate dehydrogenase, NADH diaphorase, pseudocholinesterase (E1 and E2 locus), and serum alkaline phosphatase. The most relevant observations are: (1) A relative lack of polymorphism, a characteristic feature of the Amerindian populations studied up to now. These data are consistent with the hypothesis of a 'common ancestral background' in Indian populations whatever the degree of sociocultural and linguistic diversity, and the geographical distances. (2) Specific traits due to the frequency of alleles in some systems confer to that tribe a particular position among Amerindians. The effects of genetic drift may be postulated in order to explain the high rate of PGM and 6PGD polymorphism. Furthermore, in that small community, the disappearance of some alleles (pa gene) can plausibly be explained in terms of a balanced influence of mutational and selective pressure.", "contents": "Serum and red cell enzyme variants in an Amerindian tribe: the Sirionos (Eastern Bolivia). Blood samples from 109 Siriono (Eastern Bolivia) belonging to the Tupi-Guarani group were investigated for enzyme variants in the following systems: glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, adenylate kinase, phospho-glucomutase (locus 1 and 2), acid phosphatases, lactate dehydrogenase, NADH diaphorase, pseudocholinesterase (E1 and E2 locus), and serum alkaline phosphatase. The most relevant observations are: (1) A relative lack of polymorphism, a characteristic feature of the Amerindian populations studied up to now. These data are consistent with the hypothesis of a 'common ancestral background' in Indian populations whatever the degree of sociocultural and linguistic diversity, and the geographical distances. (2) Specific traits due to the frequency of alleles in some systems confer to that tribe a particular position among Amerindians. The effects of genetic drift may be postulated in order to explain the high rate of PGM and 6PGD polymorphism. Furthermore, in that small community, the disappearance of some alleles (pa gene) can plausibly be explained in terms of a balanced influence of mutational and selective pressure.", "PMID": 976993}
{"id": "pubmed23n0032_10963", "title": "Neutral alleles at X-linked loci. A cautionary note.", "content": "Under the same conditions which yield 1 + 4Nemu as the effective number of neutral alleles maintained by fresh mutation at a rate mu at an autosomal locus in a population of size Ne, the corresponding number at an X-linked locus is 1 + 3Nemu. The resultant bimodal frequency distribution for the effective number of alleles does not seem to have been used as the basis for analysis of data on actual numbers of alleles in natural populations.", "contents": "Neutral alleles at X-linked loci. A cautionary note. Under the same conditions which yield 1 + 4Nemu as the effective number of neutral alleles maintained by fresh mutation at a rate mu at an autosomal locus in a population of size Ne, the corresponding number at an X-linked locus is 1 + 3Nemu. The resultant bimodal frequency distribution for the effective number of alleles does not seem to have been used as the basis for analysis of data on actual numbers of alleles in natural populations.", "PMID": 976994}
{"id": "pubmed23n0032_10964", "title": "Linkage relations of the loci for Kell and phenylthiocarbamide taste sensitivity.", "content": "Linkage analysis of Kell:PTC loci demonstration close linkage, theta = 0.045 with a lod score of 10.78. The results, which include the Sutter blood group, support the hypothesis that Sutter is a part of the Kell system.", "contents": "Linkage relations of the loci for Kell and phenylthiocarbamide taste sensitivity. Linkage analysis of Kell:PTC loci demonstration close linkage, theta = 0.045 with a lod score of 10.78. The results, which include the Sutter blood group, support the hypothesis that Sutter is a part of the Kell system.", "PMID": 976995}
{"id": "pubmed23n0032_10965", "title": "Distribution of G6PD types in the population of southwest France: common variants and new variants.", "content": "Glucose-6-phosphate dehydrogenase was purified from blood samples originating from 33 subjects living in the 'Midi-Pyrenees' region (Southwest France). Biochemical and electrophoretic characteristics of several enzymatic variants were revealed. The distribution of the mutations in the subjects studied gave the following results: (1) Meditteranean Gd(-) and Gd(-)A identified variants; they constitute the main part of our observations. (2) Two original variants confirmed by the WHO International Reference Laboratory, that is: Gd(-) Toulouse and Gd(+) Luz-Saint-Sauveur; the latter mutation did not produce any haematological disorder. Absence of chronic haemolytic anaemia as well as a high rate of favism could be found in the families studied. As it is generally observed in Mediterranean regions, Southwest France population is characterized by a certain heterogeneity in biochemical and genetic mutations.", "contents": "Distribution of G6PD types in the population of southwest France: common variants and new variants. Glucose-6-phosphate dehydrogenase was purified from blood samples originating from 33 subjects living in the 'Midi-Pyrenees' region (Southwest France). Biochemical and electrophoretic characteristics of several enzymatic variants were revealed. The distribution of the mutations in the subjects studied gave the following results: (1) Meditteranean Gd(-) and Gd(-)A identified variants; they constitute the main part of our observations. (2) Two original variants confirmed by the WHO International Reference Laboratory, that is: Gd(-) Toulouse and Gd(+) Luz-Saint-Sauveur; the latter mutation did not produce any haematological disorder. Absence of chronic haemolytic anaemia as well as a high rate of favism could be found in the families studied. As it is generally observed in Mediterranean regions, Southwest France population is characterized by a certain heterogeneity in biochemical and genetic mutations.", "PMID": 976996}
{"id": "pubmed23n0032_10966", "title": "Finger patterns and ridge counts of patients with Klinefelter's syndrome (47, XXY) among the Japanese.", "content": "Finger prints of a series of 48 Japanese patients with Klinefelter's syndrome (47, XXY) were studied as to frequencies of patterns and ridge counts. The patients displayed frequencies of the finger patterns on all digits similar to those of normal female controls. The finger patterns on individual digits of the patients, nevertheless, showed frequencies distinguishable from those of both normal male and female controls; arch pattern on digit I was significantly higher in incidence in Klinefelter's syndrome, whereas on digit II, ulnar loops were significantly more frequent and whorls less frequent in the patients. The patients were also noted to show significantly lower ridge counts for loops and whorls as compared with the normal controls. In Klinefelter's syndrome, significant diminution of the total finger ridge counts was demonstrable and this has been attributed not so much to the increased incidence of arches as to the frequent occurence of patterns with low ridge counts. The frequencies of finger patterns on all digits similar to those of normal females, the increased occurrence of patterns with low ridge counts as compared with the normal females, noted in the present series, are the characteristic features that are in common with those reported on white patients with this syndrome.", "contents": "Finger patterns and ridge counts of patients with Klinefelter's syndrome (47, XXY) among the Japanese. Finger prints of a series of 48 Japanese patients with Klinefelter's syndrome (47, XXY) were studied as to frequencies of patterns and ridge counts. The patients displayed frequencies of the finger patterns on all digits similar to those of normal female controls. The finger patterns on individual digits of the patients, nevertheless, showed frequencies distinguishable from those of both normal male and female controls; arch pattern on digit I was significantly higher in incidence in Klinefelter's syndrome, whereas on digit II, ulnar loops were significantly more frequent and whorls less frequent in the patients. The patients were also noted to show significantly lower ridge counts for loops and whorls as compared with the normal controls. In Klinefelter's syndrome, significant diminution of the total finger ridge counts was demonstrable and this has been attributed not so much to the increased incidence of arches as to the frequent occurence of patterns with low ridge counts. The frequencies of finger patterns on all digits similar to those of normal females, the increased occurrence of patterns with low ridge counts as compared with the normal females, noted in the present series, are the characteristic features that are in common with those reported on white patients with this syndrome.", "PMID": 976997}
{"id": "pubmed23n0032_10967", "title": "Concentrations of similar finger print patterns in four race groups.", "content": "Random samples from four races were examined to determine the frequency of subjects with none, or between 5 and 10 loops or whorls; with 2 or more radial loops, and with 3 or more simple arches. Inter-racial and sex differences occur and affect the description of various concentrations as being common or unusual.", "contents": "Concentrations of similar finger print patterns in four race groups. Random samples from four races were examined to determine the frequency of subjects with none, or between 5 and 10 loops or whorls; with 2 or more radial loops, and with 3 or more simple arches. Inter-racial and sex differences occur and affect the description of various concentrations as being common or unusual.", "PMID": 976998}
{"id": "pubmed23n0032_10968", "title": "Inheritance of acridine orange R variants in human acrocentric chromosomes.", "content": "Sequential Q and acridine orange R banding were performed on 50 normal individuals to determine those individuals with the maximum number of variants by acridine orange reverse banding. In three individuals from different families, one of the two homologs of each of pairs of acrocentric chromosomes was classified as variant. The variant present in two individuals enabled unambiguous determination of the paternal or maternal origin of all 10 acrocentric chromosomes. In the third family, the variants in the third generation were sufficient to determine the origin of homologs in 5 of 10 acrocentric chromosomes. Overall, the parental origin of 36 of 50 acrocentric chromosomes (72%) could be determined. In no case was the pattern of variants found in offsprings and parents incompatible with Mendelian inheritance.", "contents": "Inheritance of acridine orange R variants in human acrocentric chromosomes. Sequential Q and acridine orange R banding were performed on 50 normal individuals to determine those individuals with the maximum number of variants by acridine orange reverse banding. In three individuals from different families, one of the two homologs of each of pairs of acrocentric chromosomes was classified as variant. The variant present in two individuals enabled unambiguous determination of the paternal or maternal origin of all 10 acrocentric chromosomes. In the third family, the variants in the third generation were sufficient to determine the origin of homologs in 5 of 10 acrocentric chromosomes. Overall, the parental origin of 36 of 50 acrocentric chromosomes (72%) could be determined. In no case was the pattern of variants found in offsprings and parents incompatible with Mendelian inheritance.", "PMID": 976999}
{"id": "pubmed23n0032_10969", "title": "The efficacy of chemical bursectomy in chickens with congenital leukosis-virus infection.", "content": "Since treatment with Freund's complete adjuvant in chickens with congenital avian leukosisvirus infection results in severe hypergammaglobulinaemia, it was of interest to determine how various means of chemical bursectomy affected such animals. Testosterone injected during the embryonic period or cyclophosphamide administered neonatally were, alone, relatively ineffectual in suppressing the response to influenza virus or production of natural rabbit haemagglutinins, nor did these treatments reliably depress IgG or IgM levels. However, the combination of both testosterone and cyclophosphamide completely suppressed antibody responses to the antigens and resulted in animals with serum immunoglobulin levels 10-(4)-10-(5) that of untreated hypergammaglobulinaemic controls.", "contents": "The efficacy of chemical bursectomy in chickens with congenital leukosis-virus infection. Since treatment with Freund's complete adjuvant in chickens with congenital avian leukosisvirus infection results in severe hypergammaglobulinaemia, it was of interest to determine how various means of chemical bursectomy affected such animals. Testosterone injected during the embryonic period or cyclophosphamide administered neonatally were, alone, relatively ineffectual in suppressing the response to influenza virus or production of natural rabbit haemagglutinins, nor did these treatments reliably depress IgG or IgM levels. However, the combination of both testosterone and cyclophosphamide completely suppressed antibody responses to the antigens and resulted in animals with serum immunoglobulin levels 10-(4)-10-(5) that of untreated hypergammaglobulinaemic controls.", "PMID": 977031}
{"id": "pubmed23n0032_10970", "title": "The use of the radioimmunoassay in the characterization of antibodies to basement membrane collagen.", "content": "This study describes the use of the radioimmunoassay for the characterization of antibodies to basement membrane (type IV) collagen from bovine anterior lens capsule. The immunogen was extracted from calf anterior lens capsules by limited pepsin digestion and injected into rabbits. The antisera were characterized using gel diffusion, haemagglutination and the radioimmunoassay in which 125I-labelled types I, II, III, and IV bovine collagen were employed. In the direct radioimmunoassay there was no reaction with either native or denatured types I, II or III bovine collagen, whereas there were high titres towards both native and denatured type IV bovine collagen. Radioimmune inhibition studies using unlabelled types I, II, III and IV bovine collagen, collagenase digested and repepsinized type IV collagen showed that there was marked inhibition by either native, denatured or repepsinized type IV collagen, and slight inhibition by native type I collagen; native type II and type III, denatured types I, II and III, and collagenase digested type IV collagen had no inhibitory effects.", "contents": "The use of the radioimmunoassay in the characterization of antibodies to basement membrane collagen. This study describes the use of the radioimmunoassay for the characterization of antibodies to basement membrane (type IV) collagen from bovine anterior lens capsule. The immunogen was extracted from calf anterior lens capsules by limited pepsin digestion and injected into rabbits. The antisera were characterized using gel diffusion, haemagglutination and the radioimmunoassay in which 125I-labelled types I, II, III, and IV bovine collagen were employed. In the direct radioimmunoassay there was no reaction with either native or denatured types I, II or III bovine collagen, whereas there were high titres towards both native and denatured type IV bovine collagen. Radioimmune inhibition studies using unlabelled types I, II, III and IV bovine collagen, collagenase digested and repepsinized type IV collagen showed that there was marked inhibition by either native, denatured or repepsinized type IV collagen, and slight inhibition by native type I collagen; native type II and type III, denatured types I, II and III, and collagenase digested type IV collagen had no inhibitory effects.", "PMID": 977032}
{"id": "pubmed23n0032_10971", "title": "On the blood group B gene-specified alpha-galactosyltransferase in the serum of the Japanese tortoise (Clemmys japonica).", "content": "Human blood group O cells were converted into B-active cells by incubation at either 4 degrees or 37 degrees with uridine diphosphate D-galactose and unfractionated serum from the Japanese tortoise (Clemmys japonica). The specificity of the converted cells was tested by their reactions with human anti-B, anti-A and rabbit anti-B sera. Under the conditions used, Bombay (Oh) type cells were not converted, and group-O foetal cells possessing a few H antigenic sites were only weakly converted into B-active cells. In addition, the agglutination titre of the converted cells with eel anti-H serum decreased. These results therefore indicate that the conversion depends upon the preference of H-substance on the red cells. The alpha-galactosyltransferase in tortoise serum thus resembles the transferase in human group-B serum and these results suggest that the mechanism of biosynthesis of blood group B substance in the tortoise is similar to that in humans.", "contents": "On the blood group B gene-specified alpha-galactosyltransferase in the serum of the Japanese tortoise (Clemmys japonica). Human blood group O cells were converted into B-active cells by incubation at either 4 degrees or 37 degrees with uridine diphosphate D-galactose and unfractionated serum from the Japanese tortoise (Clemmys japonica). The specificity of the converted cells was tested by their reactions with human anti-B, anti-A and rabbit anti-B sera. Under the conditions used, Bombay (Oh) type cells were not converted, and group-O foetal cells possessing a few H antigenic sites were only weakly converted into B-active cells. In addition, the agglutination titre of the converted cells with eel anti-H serum decreased. These results therefore indicate that the conversion depends upon the preference of H-substance on the red cells. The alpha-galactosyltransferase in tortoise serum thus resembles the transferase in human group-B serum and these results suggest that the mechanism of biosynthesis of blood group B substance in the tortoise is similar to that in humans.", "PMID": 977033}
{"id": "pubmed23n0032_10972", "title": "Autoreactivity developing spontaneously in cultured mouse spleen cells. I. Evidence that cytotoxicity is directed against embryo-associated antigen.", "content": "We have investigated the ability of male-mouse spleen cells before and after culture in the absence of deliberate antigenic stimulation to show specific cytotoxicity to syngeneic embryo-fibroblast cells. The data suggest that cytotoxicity which develops spontaneously in such spleen cell cultures is directed primarily against embryo-associated antigens. Syngeneic Con-A-stimulated spleen cells, which, unlike fresh normal spleen cells, are also lysed by rabbit anti-mouse embryo antisera, are also a suitable target to demonstrate spontaneously developing cytotoxicity.", "contents": "Autoreactivity developing spontaneously in cultured mouse spleen cells. I. Evidence that cytotoxicity is directed against embryo-associated antigen. We have investigated the ability of male-mouse spleen cells before and after culture in the absence of deliberate antigenic stimulation to show specific cytotoxicity to syngeneic embryo-fibroblast cells. The data suggest that cytotoxicity which develops spontaneously in such spleen cell cultures is directed primarily against embryo-associated antigens. Syngeneic Con-A-stimulated spleen cells, which, unlike fresh normal spleen cells, are also lysed by rabbit anti-mouse embryo antisera, are also a suitable target to demonstrate spontaneously developing cytotoxicity.", "PMID": 977034}
{"id": "pubmed23n0032_10973", "title": "Role of catecholamines in angiotensin induced hyperglycaemia.", "content": "The effects of angiotensin II were studied on blood sugar level in dogs. Intracerebroventricular administration of pressor dose of angiotensin caused a rise in blood sugar level. The hyperglycaemia and pressor response was not affected by bilateral vagotomy and was less marked in adrenalectomized dogs. The hyperglycaemic effect and pressor response was not observed in reserpinized and spinal vagotomized dogs. It is suggested that centrally administered angiotensin stimulates the hypothalamic or medullary accelerator neurons (central sympathetic structures) to cause a marked release of catecholamines from peripheral stores specially adrenal medulla. This excessive release of catecholamines is responsible for hyperglycaemia and pressor response of angiotensin II in dogs.", "contents": "Role of catecholamines in angiotensin induced hyperglycaemia. The effects of angiotensin II were studied on blood sugar level in dogs. Intracerebroventricular administration of pressor dose of angiotensin caused a rise in blood sugar level. The hyperglycaemia and pressor response was not affected by bilateral vagotomy and was less marked in adrenalectomized dogs. The hyperglycaemic effect and pressor response was not observed in reserpinized and spinal vagotomized dogs. It is suggested that centrally administered angiotensin stimulates the hypothalamic or medullary accelerator neurons (central sympathetic structures) to cause a marked release of catecholamines from peripheral stores specially adrenal medulla. This excessive release of catecholamines is responsible for hyperglycaemia and pressor response of angiotensin II in dogs.", "PMID": 977079}
{"id": "pubmed23n0032_10974", "title": "Centrally mediated hypocholesterolaemic response of insulin in dogs.", "content": "Insulin on intravenous administration in dogs caused a rise in blood cholesterol level. This may be due to its direct action on liver or other peripheral structures. On the other hand, insulin administered into the lateral cerebral ventricles in normal as well as in spinal and vagotomized dogs resulted in a lowering of blood cholesterol. In cross circulation studies insulin administered into lateral cerebral ventricles of donor dogs produced a hypocholesterolaemia in the recepient dogs without significant changes in blood cholesterol of donor dogs. It is suggested that some substance may be liberated from some parts of central nervous system due to an action of centrally administered insulin. This substance in turn causes hypocholesterolaemia by acting on liver or some other peripheral structures in dogs.", "contents": "Centrally mediated hypocholesterolaemic response of insulin in dogs. Insulin on intravenous administration in dogs caused a rise in blood cholesterol level. This may be due to its direct action on liver or other peripheral structures. On the other hand, insulin administered into the lateral cerebral ventricles in normal as well as in spinal and vagotomized dogs resulted in a lowering of blood cholesterol. In cross circulation studies insulin administered into lateral cerebral ventricles of donor dogs produced a hypocholesterolaemia in the recepient dogs without significant changes in blood cholesterol of donor dogs. It is suggested that some substance may be liberated from some parts of central nervous system due to an action of centrally administered insulin. This substance in turn causes hypocholesterolaemia by acting on liver or some other peripheral structures in dogs.", "PMID": 977080}
{"id": "pubmed23n0032_10975", "title": "Effect of 2,4-dichlorophenoxy acetic acid on rabbit duodenum.", "content": "2,4-dichlorophenoxy acetic acid (2,4-D) produces myotonia in healthy animals. The action of this drug was studied on smooth muscles in vitro using isolated strips of rabbit's duodenum. The drug was found to have a stimulant action on the smooth muscle. The action seems to be a direct one.", "contents": "Effect of 2,4-dichlorophenoxy acetic acid on rabbit duodenum. 2,4-dichlorophenoxy acetic acid (2,4-D) produces myotonia in healthy animals. The action of this drug was studied on smooth muscles in vitro using isolated strips of rabbit's duodenum. The drug was found to have a stimulant action on the smooth muscle. The action seems to be a direct one.", "PMID": 977082}
{"id": "pubmed23n0032_10976", "title": "Effects of whole body Co-60 irradiation on the testes and epididymides of hedgehog (Hemiechinus auritus Collaris): a biochemical analysis.", "content": "The effects of 575 and 875 Rontgen (R) of whole-body irradiation in common Indian hedgehog (Hemiechinus auritus Collaris) were studied. At 875 R it showed typical signs and symptoms of radiation sickness, transient body loss and radiation mortality. Extensive vasculitis, pyknosis, chromatorrhexix and chromatolysis was noticed in the testes of irradiated hedgehogs. Epididymides and vas deferens were devoid of spermatozoa. Whole body irradiation inhibited the RNA, protein and sialic acid synthesis in the testes. Total cholesterol per gram of testes was increased significantly. Sialic acid contents of the irradiated epididymides were low. Reduced androgen production was confirmed in non-active Leydig cells, with diminished sex accessory organs and decreased fructose contents in the seminal vesicles of irradiated hedgehogs.", "contents": "Effects of whole body Co-60 irradiation on the testes and epididymides of hedgehog (Hemiechinus auritus Collaris): a biochemical analysis. The effects of 575 and 875 Rontgen (R) of whole-body irradiation in common Indian hedgehog (Hemiechinus auritus Collaris) were studied. At 875 R it showed typical signs and symptoms of radiation sickness, transient body loss and radiation mortality. Extensive vasculitis, pyknosis, chromatorrhexix and chromatolysis was noticed in the testes of irradiated hedgehogs. Epididymides and vas deferens were devoid of spermatozoa. Whole body irradiation inhibited the RNA, protein and sialic acid synthesis in the testes. Total cholesterol per gram of testes was increased significantly. Sialic acid contents of the irradiated epididymides were low. Reduced androgen production was confirmed in non-active Leydig cells, with diminished sex accessory organs and decreased fructose contents in the seminal vesicles of irradiated hedgehogs.", "PMID": 977081}
{"id": "pubmed23n0032_10977", "title": "Effect of intracerebroventricular injection of histamine on blood sugar level and hypothalamo-pituitary adrenal axis of rats.", "content": "Intraventricular injection of histamine and normal saline in rats caused a marked fall in adrenal ascorbic acid indicating a stimulatory effect of both on pituitary adrenal axis. Intraventricularly injected histamine caused significant hypoglycaemia also in rats as compared to control series.", "contents": "Effect of intracerebroventricular injection of histamine on blood sugar level and hypothalamo-pituitary adrenal axis of rats. Intraventricular injection of histamine and normal saline in rats caused a marked fall in adrenal ascorbic acid indicating a stimulatory effect of both on pituitary adrenal axis. Intraventricularly injected histamine caused significant hypoglycaemia also in rats as compared to control series.", "PMID": 977086}
{"id": "pubmed23n0032_10978", "title": "A preliminary report on the antiarrhythmic action of some newly synthesized glutarimide compounds.", "content": "The effects of a new series of glutarimide compounds have been studied in acetylcholine induced auricular fibrillation in anaesthetized cats and epinephrine induced ventricular arrhythmmias in conscious pigeons. Some of the compounds showed varying degree of protective action against experimental arrhythmias. However these compounds were found to be less potent than quinidine. The mechanism of antiarrhythmic action has been discussed.", "contents": "A preliminary report on the antiarrhythmic action of some newly synthesized glutarimide compounds. The effects of a new series of glutarimide compounds have been studied in acetylcholine induced auricular fibrillation in anaesthetized cats and epinephrine induced ventricular arrhythmmias in conscious pigeons. Some of the compounds showed varying degree of protective action against experimental arrhythmias. However these compounds were found to be less potent than quinidine. The mechanism of antiarrhythmic action has been discussed.", "PMID": 977087}
{"id": "pubmed23n0032_10979", "title": "Acetylcholine content of the brain and visceral organs of the teleostean fish; Heteropneustes fossilis and Clarias batrachus.", "content": "The acetylcholine content of various organs of two teleostean fish, Heteropneustes fossilis and Clarias batrachus has been estimated biologically on the eserinized frog rectus muscle. The acetylcholine content in the stomach of both the fish was 0.341 and 0.22 mug/g, respectively. Values were lower than those of other parts of the alimentary tract. The ilia contained the most acetylcholine, being 0.644 and 0.551 mug/g respectively, for the two fish. In the brain, heart, kidney and liver of both the fish, a considerable amount of acetylcholine was detected. Acetylcholine was not found in the spleen of Clarias batrachus, but it was present in the spleen of Heteropneustes fossilis.", "contents": "Acetylcholine content of the brain and visceral organs of the teleostean fish; Heteropneustes fossilis and Clarias batrachus. The acetylcholine content of various organs of two teleostean fish, Heteropneustes fossilis and Clarias batrachus has been estimated biologically on the eserinized frog rectus muscle. The acetylcholine content in the stomach of both the fish was 0.341 and 0.22 mug/g, respectively. Values were lower than those of other parts of the alimentary tract. The ilia contained the most acetylcholine, being 0.644 and 0.551 mug/g respectively, for the two fish. In the brain, heart, kidney and liver of both the fish, a considerable amount of acetylcholine was detected. Acetylcholine was not found in the spleen of Clarias batrachus, but it was present in the spleen of Heteropneustes fossilis.", "PMID": 977088}
{"id": "pubmed23n0032_10980", "title": "Comparative studies on different small intestinal oligosaccharidase activities in some vertebrates and invertebrates like molluscs.", "content": "Oligosaccharidase activities of the small intestinal mucosal homogenates were measured in vertebrates viz fish, toad, garden-lizard (calotes), pigeon, rat and some invertebrates viz, molluscsa. Maximum activities of the enzymes Lactase, Sucrase and Maltase were found in the mammalian species rat, whereas much less activities were found in the non-mammalian vertebrates among which toad shows the highest values and garden lizard the lowest. Among the invertebrates Pila globosa shows higher values of all the enzymes than Achatina fulica. The results obtained have been discussed in the lights of phylogeny and diet habits.", "contents": "Comparative studies on different small intestinal oligosaccharidase activities in some vertebrates and invertebrates like molluscs. Oligosaccharidase activities of the small intestinal mucosal homogenates were measured in vertebrates viz fish, toad, garden-lizard (calotes), pigeon, rat and some invertebrates viz, molluscsa. Maximum activities of the enzymes Lactase, Sucrase and Maltase were found in the mammalian species rat, whereas much less activities were found in the non-mammalian vertebrates among which toad shows the highest values and garden lizard the lowest. Among the invertebrates Pila globosa shows higher values of all the enzymes than Achatina fulica. The results obtained have been discussed in the lights of phylogeny and diet habits.", "PMID": 977083}
{"id": "pubmed23n0032_10981", "title": "Effect of autonomic drugs on the stress induced acute gastric ulceration in rat.", "content": "Stress ulcers were induced by subjecting rats to forced immobilisation and cold (4 degrees to 7 degrees C) for 4 hrs. The nature of drugs which afford varying degrees of protection against such ulceration, bring out the complexity of the factors involved in the study of pathogenesis of acute gastric ulceration due to stress.", "contents": "Effect of autonomic drugs on the stress induced acute gastric ulceration in rat. Stress ulcers were induced by subjecting rats to forced immobilisation and cold (4 degrees to 7 degrees C) for 4 hrs. The nature of drugs which afford varying degrees of protection against such ulceration, bring out the complexity of the factors involved in the study of pathogenesis of acute gastric ulceration due to stress.", "PMID": 977084}
{"id": "pubmed23n0032_10982", "title": "Colorimetric determination of piperazine.", "content": "A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.", "contents": "Colorimetric determination of piperazine. A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.", "PMID": 977089}
{"id": "pubmed23n0032_10983", "title": "Studies on the toxic effects of streptolysin 'O': effect on the contractility of isolated and intact mammalian and amphibian heart.", "content": "The effect of streptolysin O (a streptococcal exotoxin) on the myocardial contractility of isolated and intact mammalian and amphibian heart has been investigated. Streptolysin O caused marked reduction or complete cessation of myocardial contractility of mammalian and amphibian heart both in vivo and in vitro. The effect of submaximal doses of streptolysin O on isolated atria was reversible after repeated washings and the myocardial depressant effect of streptolysin O on isolated atria was reversible after repeated washings and the myocardial depressant effect of streptolysin O was not antagonised by atropine. These observations would suggest that streptolysin O is cardiotoxic and may be involved in the causation of myocardial failure associated with acute rheumatic fever in man.", "contents": "Studies on the toxic effects of streptolysin 'O': effect on the contractility of isolated and intact mammalian and amphibian heart. The effect of streptolysin O (a streptococcal exotoxin) on the myocardial contractility of isolated and intact mammalian and amphibian heart has been investigated. Streptolysin O caused marked reduction or complete cessation of myocardial contractility of mammalian and amphibian heart both in vivo and in vitro. The effect of submaximal doses of streptolysin O on isolated atria was reversible after repeated washings and the myocardial depressant effect of streptolysin O on isolated atria was reversible after repeated washings and the myocardial depressant effect of streptolysin O was not antagonised by atropine. These observations would suggest that streptolysin O is cardiotoxic and may be involved in the causation of myocardial failure associated with acute rheumatic fever in man.", "PMID": 977085}
{"id": "pubmed23n0032_10984", "title": "Relationship of capsular type to biochemical and immunological properties of teichoic acid preparations from unencapsulated strains of Staphylococcus aureus.", "content": "We investigated the biochemical and immunological characteristics of teichoic acid preparations (TAP) obtained from four unencapsulated strains of Staphylococcus aureus which nonetheless, according to the serum-soft agar technique, produced capsular type antigen and were representative of the four types A, B, C, and D. In the agar diffusion test, TAP of each strain produced a single precipitin line only against rabbit antisera corresponding to the homologous capsular type; no lines were observed against antisera to the heterologous capsular type. All TAP were ribitol type except one, glycerol, prepared from a capsular type D strain. Major acetylglucosaminyl residues of TAP from strains having capsular type A and C antigens were attached to the polyribitol phosphate by beta-linkage, whereas TAP from a type B antigen strain had an alpha-linkage; type D antigen was attached to the polyglycerol phosphate by the beta-linkage. Chemical analyses and infrared spectrograms of these TAP further confirmed their heterogeneous nature.", "contents": "Relationship of capsular type to biochemical and immunological properties of teichoic acid preparations from unencapsulated strains of Staphylococcus aureus. We investigated the biochemical and immunological characteristics of teichoic acid preparations (TAP) obtained from four unencapsulated strains of Staphylococcus aureus which nonetheless, according to the serum-soft agar technique, produced capsular type antigen and were representative of the four types A, B, C, and D. In the agar diffusion test, TAP of each strain produced a single precipitin line only against rabbit antisera corresponding to the homologous capsular type; no lines were observed against antisera to the heterologous capsular type. All TAP were ribitol type except one, glycerol, prepared from a capsular type D strain. Major acetylglucosaminyl residues of TAP from strains having capsular type A and C antigens were attached to the polyribitol phosphate by beta-linkage, whereas TAP from a type B antigen strain had an alpha-linkage; type D antigen was attached to the polyglycerol phosphate by the beta-linkage. Chemical analyses and infrared spectrograms of these TAP further confirmed their heterogeneous nature.", "PMID": 977123}
{"id": "pubmed23n0032_10985", "title": "Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity.", "content": "The intraoral establishment and proportional distribution of suspected periodontal pathogens Actinomyces viscosus and Actinomyces naeslundii were studied using a recently developed differential plating medium, CNAC-20. Saliva and dental plaque samples were collected from 108 subjects ranging in age from infants to young adults; tongue and buccal mucosa samples were collected from only the adult subjects. Catalase-negative A. naeslundii was isolated from 40% of the predentate infants' and almost all other subjects' saliva samples. It predominated among CNAC-20 isolates in the saliva of subjects of all age groups, in the plaques of young children, and in the adult tongue samples. In contrast, catalase-positive A. viscosus was not isolated from predentate infant samples, and its frequency of isolation increased slowly with age (greater than 50% detection by age 7). A. viscosus was isolated in highest relative proportions from dental plaque and buccal mucosa samples. The two closely related species A. viscosus and A. naeslundii apparently differ in respect to factors determining the host age at which they colonize and their relative intraoral distribution in humans.", "contents": "Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity. The intraoral establishment and proportional distribution of suspected periodontal pathogens Actinomyces viscosus and Actinomyces naeslundii were studied using a recently developed differential plating medium, CNAC-20. Saliva and dental plaque samples were collected from 108 subjects ranging in age from infants to young adults; tongue and buccal mucosa samples were collected from only the adult subjects. Catalase-negative A. naeslundii was isolated from 40% of the predentate infants' and almost all other subjects' saliva samples. It predominated among CNAC-20 isolates in the saliva of subjects of all age groups, in the plaques of young children, and in the adult tongue samples. In contrast, catalase-positive A. viscosus was not isolated from predentate infant samples, and its frequency of isolation increased slowly with age (greater than 50% detection by age 7). A. viscosus was isolated in highest relative proportions from dental plaque and buccal mucosa samples. The two closely related species A. viscosus and A. naeslundii apparently differ in respect to factors determining the host age at which they colonize and their relative intraoral distribution in humans.", "PMID": 977124}
{"id": "pubmed23n0032_10986", "title": "Bacterial decontamination and antileukemic therapy of AKR mice.", "content": "Four nonabsorbable antibiotics (streptomycin, neomycin, bacitracin, and amphotericin B) and a germicidal dip solution (Zephiran chloride/water) were used to eliminate all the detectable bacteria from conventional AKR mice. Control mice were not decontaminated and were used as such. When antibiotic-decontaminated and control mice developed clinical manifestations of spontaneous lymphatic leukemia, each was treated for the disease with an antitumor drug (cyclophosphamide [CP]) at weekly intervals. With the decontamination procedure, mice of each of the two groups became bacteria-free after 16 weeks of continuous oral administration of the antibiotics and two separate germicidal dippings. All decontaminated mice remained free of bacteria throughout the experiment. The bacterial flora of the control mice remained unaltered. With CP therapy, the mean survival time of the female decontaminated mice was 65 days, whereas that of male mice was 218 days. The average survival time of the CP-treated control leukemic mice was 51 days. Untreated decontaminated or control mice usually died of leukemia within 7 days after the onset of symptoms of leukemia. Although CP therapy was not curative, it did prolong the life expectancy of the decontaminated mice significantly.", "contents": "Bacterial decontamination and antileukemic therapy of AKR mice. Four nonabsorbable antibiotics (streptomycin, neomycin, bacitracin, and amphotericin B) and a germicidal dip solution (Zephiran chloride/water) were used to eliminate all the detectable bacteria from conventional AKR mice. Control mice were not decontaminated and were used as such. When antibiotic-decontaminated and control mice developed clinical manifestations of spontaneous lymphatic leukemia, each was treated for the disease with an antitumor drug (cyclophosphamide [CP]) at weekly intervals. With the decontamination procedure, mice of each of the two groups became bacteria-free after 16 weeks of continuous oral administration of the antibiotics and two separate germicidal dippings. All decontaminated mice remained free of bacteria throughout the experiment. The bacterial flora of the control mice remained unaltered. With CP therapy, the mean survival time of the female decontaminated mice was 65 days, whereas that of male mice was 218 days. The average survival time of the CP-treated control leukemic mice was 51 days. Untreated decontaminated or control mice usually died of leukemia within 7 days after the onset of symptoms of leukemia. Although CP therapy was not curative, it did prolong the life expectancy of the decontaminated mice significantly.", "PMID": 977125}
{"id": "pubmed23n0032_10987", "title": "Blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates.", "content": "Soluble sonicate supernatant preparations were made from Actinomyces viscosus (ATCC 19246), A. naeslundii (ATCC 12104), two strains of Veillonella alcalescens (strain HV-1 and a human oral isolate), Streptococcus sanguis (ATCC 10556), S. mutans (strain 6715-T2), Bacteroides melaninogenicus (strain K110), and Leptotrichia buccalis (isolated from human dental plaque). These supernatants were characterized with reference to their chemical and antigenic components and their biological activity determined by using in vitro lymphocyte blastogenesis as a measure of the host's cellular immune response. The sonicate supernatant of each bacterium contained protein, neutral sugars, methylpentose, and nucleic acids. Protein was the major component in all except L. buccalis, in which neutral sugars predominated. The antigenic components in each supernatant were detected by using rabbit antisera prepared against the whole bacteria and the sonicate supernatant. The supernatants showed a complex antigenic distribution on immunoelectrophoretic analysis. The supernatants were shown to be antigenic and not mitogenic in nature, since neither cord blood lymphocytes nor all adult lymphocytes were stimulated. The supernatant antigen preparations showed a reproducible, dose-dependent, and kinetic response in vitro, which was similar to that seen with the antigen preparation streptokinase-streptodornase.", "contents": "Blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates. Soluble sonicate supernatant preparations were made from Actinomyces viscosus (ATCC 19246), A. naeslundii (ATCC 12104), two strains of Veillonella alcalescens (strain HV-1 and a human oral isolate), Streptococcus sanguis (ATCC 10556), S. mutans (strain 6715-T2), Bacteroides melaninogenicus (strain K110), and Leptotrichia buccalis (isolated from human dental plaque). These supernatants were characterized with reference to their chemical and antigenic components and their biological activity determined by using in vitro lymphocyte blastogenesis as a measure of the host's cellular immune response. The sonicate supernatant of each bacterium contained protein, neutral sugars, methylpentose, and nucleic acids. Protein was the major component in all except L. buccalis, in which neutral sugars predominated. The antigenic components in each supernatant were detected by using rabbit antisera prepared against the whole bacteria and the sonicate supernatant. The supernatants showed a complex antigenic distribution on immunoelectrophoretic analysis. The supernatants were shown to be antigenic and not mitogenic in nature, since neither cord blood lymphocytes nor all adult lymphocytes were stimulated. The supernatant antigen preparations showed a reproducible, dose-dependent, and kinetic response in vitro, which was similar to that seen with the antigen preparation streptokinase-streptodornase.", "PMID": 977126}
{"id": "pubmed23n0032_10988", "title": "Isolation of a temperature-sensitive dengue-2 virus under conditions suitable for vaccine development.", "content": "Dengue virus, type 2, in viremic human sera and after passage in cell cultures produces mixtures of small and large plaques when assayed in LLC-MK2 cells. Clones of dengue virus type 2 obtained by plaque selection in primary green monkey kidney cell cultures were tested for temperature sensitivity in vitro and for virulence by intracerebral inoculation of suckling mice. Sublines of a small-plaque clone were found to have lower nonpermissive temperatures than the parent virus by both plaque formation and release of infectious virus into the culture media. Small-plaque sublines were significantly less virulent in suckling mice than was the parent virus. Sublines from a large-plaque clone were not temperature sensitive and closely resembled parent virus mixed-plaque morphology. When small-plaque sublines were serially passaged using undiluted inocula, reversion occurred as evidenced by the appearance of large plaques and return of mouse virulence. Small-plaque virus could be maintained through several serial passages without reversion by using low-input inocula. Desirable passage history as well as temperature-sensitive and attentuation characteristics of the S-1 small-plaque subline make it appear suitable as a vaccine candidate virus.", "contents": "Isolation of a temperature-sensitive dengue-2 virus under conditions suitable for vaccine development. Dengue virus, type 2, in viremic human sera and after passage in cell cultures produces mixtures of small and large plaques when assayed in LLC-MK2 cells. Clones of dengue virus type 2 obtained by plaque selection in primary green monkey kidney cell cultures were tested for temperature sensitivity in vitro and for virulence by intracerebral inoculation of suckling mice. Sublines of a small-plaque clone were found to have lower nonpermissive temperatures than the parent virus by both plaque formation and release of infectious virus into the culture media. Small-plaque sublines were significantly less virulent in suckling mice than was the parent virus. Sublines from a large-plaque clone were not temperature sensitive and closely resembled parent virus mixed-plaque morphology. When small-plaque sublines were serially passaged using undiluted inocula, reversion occurred as evidenced by the appearance of large plaques and return of mouse virulence. Small-plaque virus could be maintained through several serial passages without reversion by using low-input inocula. Desirable passage history as well as temperature-sensitive and attentuation characteristics of the S-1 small-plaque subline make it appear suitable as a vaccine candidate virus.", "PMID": 977127}
{"id": "pubmed23n0032_10989", "title": "Synthesis and release of sulfolipid by Mycobacterium avium during growth andcell division.", "content": "Mycobacterium avium exhibits a life cycle wherein small cells elongate to form filaments. The life cycle is unique in that elongated cells will undergo rapid division by fragmentation only if fatty acid is present. The utilization of [14C]palmitic acid and [3H]oleic acid by M. avium during the life cycle was assessed. Four glycolipids, identifiable by elution patterns from hydroxylapatite columns, were associated with postfission cells and contained isotope from the precursor fatty acid. The incorporation of 3H from oleic acid into the cellular glycolipids was maximal during cell division, but as much as 73% of the radioactivity was lost to the lipids from cells in the postfission status. Three of the glycolipids were sulfatides into which 36S was incorporated by M. avium. The [35]sulfatides were synthesized by cells undergoing fragmentation and were recovered from the medium at the termination of cell fission. These results demonstrated that the isotope was not lost to the cells because of turnover, but rather that the labeled compounds were released, intact, from the cells after fission. Because of the facile release of the sulfolipids, it was suggested that they were part of the cell envelope of M. avium cells during the division process.", "contents": "Synthesis and release of sulfolipid by Mycobacterium avium during growth andcell division. Mycobacterium avium exhibits a life cycle wherein small cells elongate to form filaments. The life cycle is unique in that elongated cells will undergo rapid division by fragmentation only if fatty acid is present. The utilization of [14C]palmitic acid and [3H]oleic acid by M. avium during the life cycle was assessed. Four glycolipids, identifiable by elution patterns from hydroxylapatite columns, were associated with postfission cells and contained isotope from the precursor fatty acid. The incorporation of 3H from oleic acid into the cellular glycolipids was maximal during cell division, but as much as 73% of the radioactivity was lost to the lipids from cells in the postfission status. Three of the glycolipids were sulfatides into which 36S was incorporated by M. avium. The [35]sulfatides were synthesized by cells undergoing fragmentation and were recovered from the medium at the termination of cell fission. These results demonstrated that the isotope was not lost to the cells because of turnover, but rather that the labeled compounds were released, intact, from the cells after fission. Because of the facile release of the sulfolipids, it was suggested that they were part of the cell envelope of M. avium cells during the division process.", "PMID": 977128}
{"id": "pubmed23n0032_10990", "title": "Radioimmunoassay for toxoplasmosis.", "content": "A solid-phase radioimmunoassay for toxoplasmosis has been developed, and the results show good correlation with the indirect hemagglutination test.", "contents": "Radioimmunoassay for toxoplasmosis. A solid-phase radioimmunoassay for toxoplasmosis has been developed, and the results show good correlation with the indirect hemagglutination test.", "PMID": 977129}
{"id": "pubmed23n0032_10991", "title": "Chromosomal determinants for exfoliative toxin production in two strains of staphylococci.", "content": "Strain TG is a plasmidless exfoliative toxin (ET) producer. Strain ER201 contains a plasmid, whose loss had no apparent effect on ET production. The plasmid of ER201 was almost identical in size to the plasmid from strain UT0007, which carries an ET determinant.", "contents": "Chromosomal determinants for exfoliative toxin production in two strains of staphylococci. Strain TG is a plasmidless exfoliative toxin (ET) producer. Strain ER201 contains a plasmid, whose loss had no apparent effect on ET production. The plasmid of ER201 was almost identical in size to the plasmid from strain UT0007, which carries an ET determinant.", "PMID": 977130}
{"id": "pubmed23n0032_10992", "title": "Duration of antibody responses to common enterobacterial and O antigens of children with pyogenic peritonitis.", "content": "Children with pyogenic peritonitis folloiwng appendicits produce antibodies against the common enterobacterial antigen (CA) in unusually high titers when compared to patients with salmonellosis, shigellosis, or urinary tract infection. The duration of this antibody response was determined in 19 children observed over a period of up to 31 months after the acute illness. CA antibody titers decreased significantly in 70% of the patients during the first year and in 91% of those studied between 13 and 31 months after the infection. In the majority (71%) of patients the antibody titers returned to pre- or near pre-infection levels within 31 months after the acute illness. Only in a few patients (19%) were near maximal titers maintained during the observation period. The titers of antibodies against the O antigens of the infecting microorganisms also decreased in the majority of subjects during the follow-up period of observation. These findings may be of importance in connection with studies on the immune response of patients with urinary tract and other infections.", "contents": "Duration of antibody responses to common enterobacterial and O antigens of children with pyogenic peritonitis. Children with pyogenic peritonitis folloiwng appendicits produce antibodies against the common enterobacterial antigen (CA) in unusually high titers when compared to patients with salmonellosis, shigellosis, or urinary tract infection. The duration of this antibody response was determined in 19 children observed over a period of up to 31 months after the acute illness. CA antibody titers decreased significantly in 70% of the patients during the first year and in 91% of those studied between 13 and 31 months after the infection. In the majority (71%) of patients the antibody titers returned to pre- or near pre-infection levels within 31 months after the acute illness. Only in a few patients (19%) were near maximal titers maintained during the observation period. The titers of antibodies against the O antigens of the infecting microorganisms also decreased in the majority of subjects during the follow-up period of observation. These findings may be of importance in connection with studies on the immune response of patients with urinary tract and other infections.", "PMID": 977131}
{"id": "pubmed23n0032_10993", "title": "Clinical experience in detecting endotoxemia with the limulus test in typhoid fever and other Salmonella infections.", "content": "Semiquantitative estimates of circulating endotoxin were performed by the limulus test in patients suffering from typhoid fever and other salmonelloses. The test was positive in a large number of cases. However, no clearcut correlation was found between existence of endotoxemia, as such, and pyrexia. A correlation with recent bacteremia was found for highest levels of endotoxin activity. In minor salmonelloses a striking prevalence of positive cases was observed in the age group under one year. These findings were discussed in relation to the diagnostic and pathogenetic facets of the problem.", "contents": "Clinical experience in detecting endotoxemia with the limulus test in typhoid fever and other Salmonella infections. Semiquantitative estimates of circulating endotoxin were performed by the limulus test in patients suffering from typhoid fever and other salmonelloses. The test was positive in a large number of cases. However, no clearcut correlation was found between existence of endotoxemia, as such, and pyrexia. A correlation with recent bacteremia was found for highest levels of endotoxin activity. In minor salmonelloses a striking prevalence of positive cases was observed in the age group under one year. These findings were discussed in relation to the diagnostic and pathogenetic facets of the problem.", "PMID": 977132}
{"id": "pubmed23n0032_10994", "title": "[Pharmacokinetics of azlocillin, a new semisynthetic, wide-spectrum antibiotic (author's transl)].", "content": "Azlocillin, a new semisynthetic penicillin with a wide spectrum of antimicrobial activity and a member of the ureidopenicillin group, was administered to ten adults in a dosage of 2 g by intravenous bolus injection. The determination of antibiotic activity in serum and urine demonstrated an average concentration of 58.9 mug/ml after one hour, 28.1 mug/ml after two hours and 6.1 mug/ml after four hours. The total urinary excretion was 60% within six hours. The pharmacokinetic parameters were calculated for the one and two compartment models, respectively. Mathematical analysis according to an open two-compartment model resulted in better curve adjustment as judged from the sum of the deviant squares. The resulting parameters for volume of distribution and rate of elimination show only minor differences however. Similar results were seen on comparison with the respective data for ampicillin.", "contents": "[Pharmacokinetics of azlocillin, a new semisynthetic, wide-spectrum antibiotic (author's transl)]. Azlocillin, a new semisynthetic penicillin with a wide spectrum of antimicrobial activity and a member of the ureidopenicillin group, was administered to ten adults in a dosage of 2 g by intravenous bolus injection. The determination of antibiotic activity in serum and urine demonstrated an average concentration of 58.9 mug/ml after one hour, 28.1 mug/ml after two hours and 6.1 mug/ml after four hours. The total urinary excretion was 60% within six hours. The pharmacokinetic parameters were calculated for the one and two compartment models, respectively. Mathematical analysis according to an open two-compartment model resulted in better curve adjustment as judged from the sum of the deviant squares. The resulting parameters for volume of distribution and rate of elimination show only minor differences however. Similar results were seen on comparison with the respective data for ampicillin.", "PMID": 977133}
{"id": "pubmed23n0032_10995", "title": "Clinical study on the antifungal activity of griseofulvin in the presence of surfactants.", "content": "Scales were taken from 128 human volunteers suffering from ringworm infections and grown on Sabourand's media to determine the type of organisms causing the disease. Groups of 32 patients each were treated for sixty days with griseofulvin tablets containing dioctyl sodium sulfosuccinate, cetyl pyridinium chloride and polysorbate 80 respectively, while another group of 32 was given tablets containing griseofulvin only for the same period. The subjects treated with griseofulvin tablets containing surfactants showed a higher rate of cure than those treated with griseofulvin alone.", "contents": "Clinical study on the antifungal activity of griseofulvin in the presence of surfactants. Scales were taken from 128 human volunteers suffering from ringworm infections and grown on Sabourand's media to determine the type of organisms causing the disease. Groups of 32 patients each were treated for sixty days with griseofulvin tablets containing dioctyl sodium sulfosuccinate, cetyl pyridinium chloride and polysorbate 80 respectively, while another group of 32 was given tablets containing griseofulvin only for the same period. The subjects treated with griseofulvin tablets containing surfactants showed a higher rate of cure than those treated with griseofulvin alone.", "PMID": 977134}
{"id": "pubmed23n0032_10996", "title": "Screening for mumps immunity with the microtiter solid-phase radioimmunoassay.", "content": "A solid phase radioimmunoassay on microtiter plates was adapted for the estimation of mumps antibodies using commercially available complement-fixing mumps antigen. The sensitivity of the test is superior to that of hemagglutination inhibition and lies in the same range as the virus neutralization test performed on chick fibroblasts. The method is useful for screening large series of human sera, for instance in connection with vaccination programs.", "contents": "Screening for mumps immunity with the microtiter solid-phase radioimmunoassay. A solid phase radioimmunoassay on microtiter plates was adapted for the estimation of mumps antibodies using commercially available complement-fixing mumps antigen. The sensitivity of the test is superior to that of hemagglutination inhibition and lies in the same range as the virus neutralization test performed on chick fibroblasts. The method is useful for screening large series of human sera, for instance in connection with vaccination programs.", "PMID": 977135}
{"id": "pubmed23n0032_10997", "title": "Gonorrhoea in the asymptomatic patient: presentation and the role of contact tracing for heterosexual men and women and for homosexual men.", "content": "Clinically silent gonorrhoea is the major problem in the control of the disease. Only 12 per cent of infected women reported in 1974 because of symptoms, compared with 97 per cent of infected heterosexual men and only 35 per cent of homosexual men with gonococcal proctitis alone. Homosexual men, compared with heterosexual men, had twice as many subsequent sexual contacts after infection and had a higher incidence of early syphilis. Eighty-four per cent had experienced passive anorectal intercourse. Ninety-seven per cent of men with gonococcal urethritis reported because of symptoms, but occasionally (particularly after unsuccessful treatment) urethral gonorrhoea in men may be clinically silent and even require tests of the overnight urethral secretion for diagnosis. For women, and for homosexual men who have had passive anorectal (or oral) intercourse, the indication for attendance for tests for gonorrhoea should be having run the risk, and not the presence of symptoms. Routine tests of the anorectum for gonorrhoea are essential in cases of 80 women at risk, and for most homosexual men since over 80 per cent of these men will have had passive anorectal intercourse. Because gonococcal infections following treatment-failure are often clinically silent in both women and men, symptoms cannot be relied upon to indicate such failure. Follow-up smears and cultures are always essential.", "contents": "Gonorrhoea in the asymptomatic patient: presentation and the role of contact tracing for heterosexual men and women and for homosexual men. Clinically silent gonorrhoea is the major problem in the control of the disease. Only 12 per cent of infected women reported in 1974 because of symptoms, compared with 97 per cent of infected heterosexual men and only 35 per cent of homosexual men with gonococcal proctitis alone. Homosexual men, compared with heterosexual men, had twice as many subsequent sexual contacts after infection and had a higher incidence of early syphilis. Eighty-four per cent had experienced passive anorectal intercourse. Ninety-seven per cent of men with gonococcal urethritis reported because of symptoms, but occasionally (particularly after unsuccessful treatment) urethral gonorrhoea in men may be clinically silent and even require tests of the overnight urethral secretion for diagnosis. For women, and for homosexual men who have had passive anorectal (or oral) intercourse, the indication for attendance for tests for gonorrhoea should be having run the risk, and not the presence of symptoms. Routine tests of the anorectum for gonorrhoea are essential in cases of 80 women at risk, and for most homosexual men since over 80 per cent of these men will have had passive anorectal intercourse. Because gonococcal infections following treatment-failure are often clinically silent in both women and men, symptoms cannot be relied upon to indicate such failure. Follow-up smears and cultures are always essential.", "PMID": 977136}
{"id": "pubmed23n0032_10998", "title": "Description of a computer program for the determination of regional cerebral blood flow.", "content": "The regional cerebral blood flow in man was measured, following a short injection 133 Xenon into the internal carotid, by processing the hemispheric clearance curves detected with an Anger gamma-camera. An automatic calculation program is described which permits the estimation of the two compartmental blood flows. The merits of this procedure are discussed.", "contents": "Description of a computer program for the determination of regional cerebral blood flow. The regional cerebral blood flow in man was measured, following a short injection 133 Xenon into the internal carotid, by processing the hemispheric clearance curves detected with an Anger gamma-camera. An automatic calculation program is described which permits the estimation of the two compartmental blood flows. The merits of this procedure are discussed.", "PMID": 977173}
{"id": "pubmed23n0032_10999", "title": "Electrocochleography by mathematical modelling.", "content": "The aim of this work is to critically review some of the classical electrocochleographic methods in accordance with the systems theory: (a) in describing, by means of mathematical models, the different parts of the auditory system concerned in the generation of stimulus-evoked signals; and: (b) in comparing the outputs of these models with the measurements of the cochlear potentials. It must be stated clearly that we realise that many of the conclusions drawn could be criticised, it is hoped that future research in the field with be stimulated by this work.", "contents": "Electrocochleography by mathematical modelling. The aim of this work is to critically review some of the classical electrocochleographic methods in accordance with the systems theory: (a) in describing, by means of mathematical models, the different parts of the auditory system concerned in the generation of stimulus-evoked signals; and: (b) in comparing the outputs of these models with the measurements of the cochlear potentials. It must be stated clearly that we realise that many of the conclusions drawn could be criticised, it is hoped that future research in the field with be stimulated by this work.", "PMID": 977174}
{"id": "pubmed23n0032_11000", "title": "Computer-aided anaesthesia administration.", "content": "Certain repetitive tasks of the anaesthesiologist are studied and modelled for automatic processing. These analyses make it possible for optimal control procedures to be programmed and administered by less trained anaesthesia professionals. A dynamic programming model is developed and validated with real data collected from several hospitals in the Cleveland, Ohio, area. By conjunctively monitoring certain transients in the anaesthesia administration process, the scarce supply of anaesthesiology manpower can be more efficiently deployed.", "contents": "Computer-aided anaesthesia administration. Certain repetitive tasks of the anaesthesiologist are studied and modelled for automatic processing. These analyses make it possible for optimal control procedures to be programmed and administered by less trained anaesthesia professionals. A dynamic programming model is developed and validated with real data collected from several hospitals in the Cleveland, Ohio, area. By conjunctively monitoring certain transients in the anaesthesia administration process, the scarce supply of anaesthesiology manpower can be more efficiently deployed.", "PMID": 977175}
{"id": "pubmed23n0032_11001", "title": "Implications of fertility patterns in the Republic of Korea: a study by computer simulation.", "content": "Implications of various fertility patterns in the Republic of Korea (ROK) are studied by computer simulation. These patterns include the factors: age of marriage, sex preference, abortion (induced), contraception and sterilisation. As a result of studying a large number of combinations of these factors, the following tentative conclusions, among others, are reached. If future cohorts of women in the ROK become limiters after two live births and marry according to an observed high level marriage distribution, at least two of the methods of birth control--abortion, contraception, and sterilisation--will have to be used extensively to yield a mean number of live births (MNLB) in these cohorts of the order 2-2. However, if these women marry later than the observed high level marriage distribution and, in addition, accept effective methods of contraception more widely and use them more effectively, then the MNLB experienced by these cohorts could move below 2-5 wihtout the extensive utilisation of abortion and sterilisation.", "contents": "Implications of fertility patterns in the Republic of Korea: a study by computer simulation. Implications of various fertility patterns in the Republic of Korea (ROK) are studied by computer simulation. These patterns include the factors: age of marriage, sex preference, abortion (induced), contraception and sterilisation. As a result of studying a large number of combinations of these factors, the following tentative conclusions, among others, are reached. If future cohorts of women in the ROK become limiters after two live births and marry according to an observed high level marriage distribution, at least two of the methods of birth control--abortion, contraception, and sterilisation--will have to be used extensively to yield a mean number of live births (MNLB) in these cohorts of the order 2-2. However, if these women marry later than the observed high level marriage distribution and, in addition, accept effective methods of contraception more widely and use them more effectively, then the MNLB experienced by these cohorts could move below 2-5 wihtout the extensive utilisation of abortion and sterilisation.", "PMID": 977176}
{"id": "pubmed23n0032_11002", "title": "Semiautomatic processing of neurophysiological data: part I--a device for measuring potential intervals from film.", "content": "This paper describes the operation and construction of an electromechanical device designed to make measurements on oscillograph recordings of nerve or muscle potentials for subsequent computer analysis. The device consists of a motor-driven shaft which moves the record past a fixed cursor, and an electronic counter which records the movements of the shaft, thereby providing a cumulative tally of the distance of the current position of the cursor from some arbitrary origin on the record. When the device is connected to a paper tape punch, the operator can punch the distance measurement associated with potential along the record onto the tape. The format of data on the tape readily allows further analysis by computer.", "contents": "Semiautomatic processing of neurophysiological data: part I--a device for measuring potential intervals from film. This paper describes the operation and construction of an electromechanical device designed to make measurements on oscillograph recordings of nerve or muscle potentials for subsequent computer analysis. The device consists of a motor-driven shaft which moves the record past a fixed cursor, and an electronic counter which records the movements of the shaft, thereby providing a cumulative tally of the distance of the current position of the cursor from some arbitrary origin on the record. When the device is connected to a paper tape punch, the operator can punch the distance measurement associated with potential along the record onto the tape. The format of data on the tape readily allows further analysis by computer.", "PMID": 977177}
{"id": "pubmed23n0032_11003", "title": "Immunosuppression in Burkitt's lymphoma. II. Peripheral blood lymphocyte populations related to clinical status.", "content": "We have studied peripheral blood lymphocyte populations, defined in terms of their E and EAC' rosette-forming capacity in patients with Burkitt's lymphoma and controls. Total lymphocyte counts were reduced in patients compared to controls (p less than 0.005), and correlated with clinical stage and disease status. Presenting patients with Stage D tumours had the lowest levels, while patients in remission for at least 18 months had total lymphocyte counts similar to those of controls. Absolute numbers of both E and EAC' rosette-forming cells (RFC) were reduced, compared to controls, in tumour-bearing patients (p less than 0.0005 for both E and EAC' RFC) and also, to a lesser extent, in patients in remission (p less than 0.025 and less than 0.05 for E and EAC' RFC respectively). In the case of E RFC a significant reduction was present even when patients in remission for over 18 months were considered alone (p less than 0.05). Non-RFC were present in similar numbers in patients and controls, so that the reduction in total lymphocyte count can be accounted for entirely by the reduced numbers of RFC. As anticipated by this, percentages of RFC were also reduced in patients compared to controls, EAC' RFC to a lesser extent than E RFC. Non-RFC percentages were correspondingly increased. Tumour-bearing patients had significantly impaired PHA responses and cutaneous reactivity, which correlated significantly with very low levels of RFC. One possible explanation for these results is that immunoreactive lymphocytes are sequestered within the tumour. This is consistent with the immunosuppression observed in tumour-bearing patients, and would also result in difficulty in detecting even a powerful tumour-specific immune reaction by means of an assay dependent upon the participation of peripheral blood lymphocytes.", "contents": "Immunosuppression in Burkitt's lymphoma. II. Peripheral blood lymphocyte populations related to clinical status. We have studied peripheral blood lymphocyte populations, defined in terms of their E and EAC' rosette-forming capacity in patients with Burkitt's lymphoma and controls. Total lymphocyte counts were reduced in patients compared to controls (p less than 0.005), and correlated with clinical stage and disease status. Presenting patients with Stage D tumours had the lowest levels, while patients in remission for at least 18 months had total lymphocyte counts similar to those of controls. Absolute numbers of both E and EAC' rosette-forming cells (RFC) were reduced, compared to controls, in tumour-bearing patients (p less than 0.0005 for both E and EAC' RFC) and also, to a lesser extent, in patients in remission (p less than 0.025 and less than 0.05 for E and EAC' RFC respectively). In the case of E RFC a significant reduction was present even when patients in remission for over 18 months were considered alone (p less than 0.05). Non-RFC were present in similar numbers in patients and controls, so that the reduction in total lymphocyte count can be accounted for entirely by the reduced numbers of RFC. As anticipated by this, percentages of RFC were also reduced in patients compared to controls, EAC' RFC to a lesser extent than E RFC. Non-RFC percentages were correspondingly increased. Tumour-bearing patients had significantly impaired PHA responses and cutaneous reactivity, which correlated significantly with very low levels of RFC. One possible explanation for these results is that immunoreactive lymphocytes are sequestered within the tumour. This is consistent with the immunosuppression observed in tumour-bearing patients, and would also result in difficulty in detecting even a powerful tumour-specific immune reaction by means of an assay dependent upon the participation of peripheral blood lymphocytes.", "PMID": 977186}
{"id": "pubmed23n0032_11004", "title": "Cancer incidence in Alaska natives.", "content": "A survey of cancer incidence among Alaska Natives for the 5-year period 1969-73 revealed fewer cancer cases overall than expected in relation to US rates, but significantly increased risk for certain cancer sites: the nasopharynx in both sexes (with excesses over 15-fold), the liver in males, and the salivary glands, gallbladder, kidney and thyroid in females. Compared with earlier reports, the observations suggest marked changes in cancer incidence among Alaska Natives over the past two decades, with declines in esophageal and invasive cervical cancers, and increases in cancers of the lung, colon and rectum.", "contents": "Cancer incidence in Alaska natives. A survey of cancer incidence among Alaska Natives for the 5-year period 1969-73 revealed fewer cancer cases overall than expected in relation to US rates, but significantly increased risk for certain cancer sites: the nasopharynx in both sexes (with excesses over 15-fold), the liver in males, and the salivary glands, gallbladder, kidney and thyroid in females. Compared with earlier reports, the observations suggest marked changes in cancer incidence among Alaska Natives over the past two decades, with declines in esophageal and invasive cervical cancers, and increases in cancers of the lung, colon and rectum.", "PMID": 977187}
{"id": "pubmed23n0032_11005", "title": "Survey of human lymphoblastoid cell lines and primary cultures of normal and leukaemic leukocytes for oncornavirus production.", "content": "Velocity sedimentation of uridine-labelled cultures was found to be more reliable than isopycnic sedimentation in detecting oncornavirus production in lymphoid cells. Of 13 cell lines (including six derivea from Burkitt's lymphomas and two from leukaemic leukocytes) only one, the leukaemia-derived, Epstein-Barr virus-producing line QIMR-WIL, showed any activity. The nature of the QIMR-WIL particles was further defined by isolation of uridine-labelled 70S RNA and by the simultaneous assay for reverse transcriptase and 70S RNA, but production of such particles was detected in only three of 10 assays. Pretreatment of cells with 5'-iododeoxyuridine or culture in arginine-free medium did not induce particle production. Syncytia assays using XC cells were negative. Of 13 primary cultures (nine samples of leukaemic leukocytes and four of cord leukocytes) treated with mitogens and subjected to inducing conditions, one (leukocytes from a patient with acute myelogenous leukaemia) showed evidence in successive assays of oncornavirus synthesis. The low and transient yield of oncornavirus-like particles obtained in this work parallels that reported in previous studies of fresh lymphoid cells and primary cultures.", "contents": "Survey of human lymphoblastoid cell lines and primary cultures of normal and leukaemic leukocytes for oncornavirus production. Velocity sedimentation of uridine-labelled cultures was found to be more reliable than isopycnic sedimentation in detecting oncornavirus production in lymphoid cells. Of 13 cell lines (including six derivea from Burkitt's lymphomas and two from leukaemic leukocytes) only one, the leukaemia-derived, Epstein-Barr virus-producing line QIMR-WIL, showed any activity. The nature of the QIMR-WIL particles was further defined by isolation of uridine-labelled 70S RNA and by the simultaneous assay for reverse transcriptase and 70S RNA, but production of such particles was detected in only three of 10 assays. Pretreatment of cells with 5'-iododeoxyuridine or culture in arginine-free medium did not induce particle production. Syncytia assays using XC cells were negative. Of 13 primary cultures (nine samples of leukaemic leukocytes and four of cord leukocytes) treated with mitogens and subjected to inducing conditions, one (leukocytes from a patient with acute myelogenous leukaemia) showed evidence in successive assays of oncornavirus synthesis. The low and transient yield of oncornavirus-like particles obtained in this work parallels that reported in previous studies of fresh lymphoid cells and primary cultures.", "PMID": 977188}
{"id": "pubmed23n0032_11006", "title": "Fc receptor-bearing cells as a reliable marker for quantitation of host lymphoreticular infiltration of progressively growing solid tumors.", "content": "Disaggregated cell suspensions made from transplanted solid tumors, either chemically-induced fibrosarcomas, or spontaneous mammary carcinomas, can contain very high numbers of Fc receptor-bearing cells which are of host origin. Because most types of lymphoreticular cells have Fc receptors, and because T cells--most of which are Fc receptor-negative--appear to infiltrate such tumors only to a very limited degree, the possibility that Fc receptor cells could serve as a reliable and simple marker for host lymphoreticular cell infiltration of solid tumors was tested. This was accomplished by comparing the ratios of Fc rosetting cells to serologically detectable host cells in H2d or H2k haplotype tumor cell suspensions grown in (H2d X H2k)f1 hybrid mice, where host cells could be distinguished from tumor cells by treatment with the appropriate anti-H2 serum. Ratios of 0.8 to 1.08 were obtained for four different tumors including the SaD/2 fibrosarcoma, a CBA spontaneous fibrosarcoma, and the T1699 and CaD/2 mammary carcinomas. Analysis of the results showed that enumeration of Fc rosettes was a reliable host cell maker for at least three of the four tumors tested. The mean non-malignant host cell content of the various tumors, as assessed by anti-H2 cytotoxicity tests, ranged from 23% to 41%.", "contents": "Fc receptor-bearing cells as a reliable marker for quantitation of host lymphoreticular infiltration of progressively growing solid tumors. Disaggregated cell suspensions made from transplanted solid tumors, either chemically-induced fibrosarcomas, or spontaneous mammary carcinomas, can contain very high numbers of Fc receptor-bearing cells which are of host origin. Because most types of lymphoreticular cells have Fc receptors, and because T cells--most of which are Fc receptor-negative--appear to infiltrate such tumors only to a very limited degree, the possibility that Fc receptor cells could serve as a reliable and simple marker for host lymphoreticular cell infiltration of solid tumors was tested. This was accomplished by comparing the ratios of Fc rosetting cells to serologically detectable host cells in H2d or H2k haplotype tumor cell suspensions grown in (H2d X H2k)f1 hybrid mice, where host cells could be distinguished from tumor cells by treatment with the appropriate anti-H2 serum. Ratios of 0.8 to 1.08 were obtained for four different tumors including the SaD/2 fibrosarcoma, a CBA spontaneous fibrosarcoma, and the T1699 and CaD/2 mammary carcinomas. Analysis of the results showed that enumeration of Fc rosettes was a reliable host cell maker for at least three of the four tumors tested. The mean non-malignant host cell content of the various tumors, as assessed by anti-H2 cytotoxicity tests, ranged from 23% to 41%.", "PMID": 977189}
{"id": "pubmed23n0032_11007", "title": "Canine testicular tumors: epidemiologic features of 410 dogs.", "content": "Histologically confirmed testicular tumors were diagnosed in 410 dogs from 12 North American veterinary university hospitals and clinics. Three tumor-cell types, Sertoli cell tumor, interstitial cell tumor and seminoma, were about equally represented. Several breeds were identified with high risk for different testicular tumor-cell types. Cytogenetic and immunogenetic studies of these dog families could offer leads applicable to familial testicular cancer in man. The multiplicity of breeds within the series suggests that, as in man, other factors, in addition to hereditary, play a role in etiology. Cryptorchid dogs appear to have a 13.6 times higher risk of testicular tumor than normal dogs. Additionally, male dogs with an inguinal hernia have an increased risk (4.7) of testis tumors. There were no detectable excesses of other urogenital anomalies or urogenital tumors among the series. The Shetland Sheepdog is suggested as an appropriate model for research into the mechanisms responsible for testicular maldescent and tumorigenesis.", "contents": "Canine testicular tumors: epidemiologic features of 410 dogs. Histologically confirmed testicular tumors were diagnosed in 410 dogs from 12 North American veterinary university hospitals and clinics. Three tumor-cell types, Sertoli cell tumor, interstitial cell tumor and seminoma, were about equally represented. Several breeds were identified with high risk for different testicular tumor-cell types. Cytogenetic and immunogenetic studies of these dog families could offer leads applicable to familial testicular cancer in man. The multiplicity of breeds within the series suggests that, as in man, other factors, in addition to hereditary, play a role in etiology. Cryptorchid dogs appear to have a 13.6 times higher risk of testicular tumor than normal dogs. Additionally, male dogs with an inguinal hernia have an increased risk (4.7) of testis tumors. There were no detectable excesses of other urogenital anomalies or urogenital tumors among the series. The Shetland Sheepdog is suggested as an appropriate model for research into the mechanisms responsible for testicular maldescent and tumorigenesis.", "PMID": 977190}
{"id": "pubmed23n0032_11008", "title": "In vitro generation of primary and secondary cytotoxic cell-mediated immune responses to chemically induced mouse sarcomas.", "content": "Mixtures of lymph node and spleen cells from normal (untreated) BALB/c mice and from BALB/c mice whose syngeneic tumors had been excised 7-28 days previously (\"tumor-excised mice\"), were sensitized in vitro by cultivation for 9 days with cells from syngeneic, methylcholanthrene-induced sarcomas. The in vitro-sensitized lymphoid cells were tested in a 36-h microcytotoxicity assay for reactivity against target cells carrying the sensitizing tumor antigens, as well as against control target cells lacking these antigens. After co-cultivation with tumor cells, lymphoid cells from both normal and tumor-excised mice were cytotoxic to tumor cells carrying the sensitizing antigens. The cytotoxicity was generally specific, and occurred at low effector: target cell ratios (in some experiments down to 1:1). When lymphoid cells from tumor-excised mice were exposed in vitro for 9 days to cells carrying the same antigens as those which were originally present on the surgical excised tumors, the effector cells obtained gave a dose-dependent cytotoxic response suggestive of a linear relationship. When lymphoid cells from normal mice were similarly sensitized for 9 days, specifically cytotoxic lymphoid cells were generated but no linear dose-dependent response was detected.", "contents": "In vitro generation of primary and secondary cytotoxic cell-mediated immune responses to chemically induced mouse sarcomas. Mixtures of lymph node and spleen cells from normal (untreated) BALB/c mice and from BALB/c mice whose syngeneic tumors had been excised 7-28 days previously (\"tumor-excised mice\"), were sensitized in vitro by cultivation for 9 days with cells from syngeneic, methylcholanthrene-induced sarcomas. The in vitro-sensitized lymphoid cells were tested in a 36-h microcytotoxicity assay for reactivity against target cells carrying the sensitizing tumor antigens, as well as against control target cells lacking these antigens. After co-cultivation with tumor cells, lymphoid cells from both normal and tumor-excised mice were cytotoxic to tumor cells carrying the sensitizing antigens. The cytotoxicity was generally specific, and occurred at low effector: target cell ratios (in some experiments down to 1:1). When lymphoid cells from tumor-excised mice were exposed in vitro for 9 days to cells carrying the same antigens as those which were originally present on the surgical excised tumors, the effector cells obtained gave a dose-dependent cytotoxic response suggestive of a linear relationship. When lymphoid cells from normal mice were similarly sensitized for 9 days, specifically cytotoxic lymphoid cells were generated but no linear dose-dependent response was detected.", "PMID": 977191}
{"id": "pubmed23n0032_11009", "title": "Lung tumour incidence in mice treated with hydrazine sulphate.", "content": "Lung tumour incidence in mice fed with hydrazine sulphate (1.1 mg/day/mouse) was studied in male and female mice of Swiss, Strong \"A\" and F1 cross of ICRC (female) X C3H (Jax) (male), as well as in C17 males. Swiss strain mice showed 100% lung adenocarcinomas. None of the treated mice of different strains had liver tumours. Hydrazine sulphate also induced adenocarcinomas of lung in Strong \"A\" and F1 cross of ICRC females X C3H (Jax) males but it produced lymphomas of lung in C17 strain. Female mice of Swiss strain and F1 hybrids showed greater susceptibility to hydrazine sulphate than the males. It was interesting to observe that protein and vitamin B deficiency in the diet shortened the tumour induction period in Swiss strain mice.", "contents": "Lung tumour incidence in mice treated with hydrazine sulphate. Lung tumour incidence in mice fed with hydrazine sulphate (1.1 mg/day/mouse) was studied in male and female mice of Swiss, Strong \"A\" and F1 cross of ICRC (female) X C3H (Jax) (male), as well as in C17 males. Swiss strain mice showed 100% lung adenocarcinomas. None of the treated mice of different strains had liver tumours. Hydrazine sulphate also induced adenocarcinomas of lung in Strong \"A\" and F1 cross of ICRC females X C3H (Jax) males but it produced lymphomas of lung in C17 strain. Female mice of Swiss strain and F1 hybrids showed greater susceptibility to hydrazine sulphate than the males. It was interesting to observe that protein and vitamin B deficiency in the diet shortened the tumour induction period in Swiss strain mice.", "PMID": 977192}
{"id": "pubmed23n0032_11010", "title": "Changes in phosphorus metabolism during the tumor growth inhibition by metabolic acidosis.", "content": "Ammonium chloride-induced metabolic acidosis produces an increase of the phosphorus turnover rate in tumor tissue. The lower blood protein-bound 32P seems to indicate that the high phospholipid turnover observed in tumor may be related to an increased molecular degradation. It seems likely that this phenomenon together with the augmented release of phospholipid-bound calcium may induce important changes in the cell membrane behavior.", "contents": "Changes in phosphorus metabolism during the tumor growth inhibition by metabolic acidosis. Ammonium chloride-induced metabolic acidosis produces an increase of the phosphorus turnover rate in tumor tissue. The lower blood protein-bound 32P seems to indicate that the high phospholipid turnover observed in tumor may be related to an increased molecular degradation. It seems likely that this phenomenon together with the augmented release of phospholipid-bound calcium may induce important changes in the cell membrane behavior.", "PMID": 977199}
{"id": "pubmed23n0032_11011", "title": "Serum calcium balance during early phase of diphenylhydantoin therapy.", "content": "The timing of anticonvulsant-induced disturbances of calcium metabolism was investigated in ten epileptics before diphenylhydantoin therapy and during it for up to three years. There was a significant decrease in average serum calcium and an increase in average serum alkaline phosphatase one month after the commencement of the therapy, these values remaining constant thereafter. Decreased serum inorganic phosphate and elevated hydroxyproline levels were also seen in the patients during the followup. At any time after the start of the drug therapy, 10 to 40 per cent of the patients had abnormalities in one or more of these parameters. The findings suggest that prophylactic therapy with vitamin D is indicated from the earliest phase of anticonvulsant therapy to prevent the development of bone disease.", "contents": "Serum calcium balance during early phase of diphenylhydantoin therapy. The timing of anticonvulsant-induced disturbances of calcium metabolism was investigated in ten epileptics before diphenylhydantoin therapy and during it for up to three years. There was a significant decrease in average serum calcium and an increase in average serum alkaline phosphatase one month after the commencement of the therapy, these values remaining constant thereafter. Decreased serum inorganic phosphate and elevated hydroxyproline levels were also seen in the patients during the followup. At any time after the start of the drug therapy, 10 to 40 per cent of the patients had abnormalities in one or more of these parameters. The findings suggest that prophylactic therapy with vitamin D is indicated from the earliest phase of anticonvulsant therapy to prevent the development of bone disease.", "PMID": 977200}
{"id": "pubmed23n0032_11012", "title": "[Psychopharmacological studies on the interactions between alcohol and oxazepam with regard to reaction behavior. 1st communication. Changes of psychomotor coordination and reaction behavior].", "content": "The interactions between oxazepam and ethanol were investigated in a pharmacopsychological study. The results of the psycho-experimental investigations are reported in this paper. The effects of oxazepam and/or ethanol doses given to 14 subjects with known blood concentrations of the respective substances were monitored and compared to placebo effects. - An analysis of the experiments with respect to the psychomotoric responses showed a tendency towards motoric instability under ethanol (A), whereas in the placebo experiment a relatively constant level of efficiency was maintained. In the oxazepam experiment (C) a slowing of the psychomotoric functions was observed. The concomitant influence of ethanol and oxazepam accentuates the motoric instability with a delay of the motoric responses. These effects remain visible, though slightly modified, after the administration of both substances at intervals (E). The experiments showed a decrease in efficiency during continuous exercise, a delayed response to optic and acoustic stimuli, and a retardation of the entire behavioral pattern under the influence of ethanol and oxazepam. The results indicate the existence of marked interactions between ethanol and oxazepam.", "contents": "[Psychopharmacological studies on the interactions between alcohol and oxazepam with regard to reaction behavior. 1st communication. Changes of psychomotor coordination and reaction behavior]. The interactions between oxazepam and ethanol were investigated in a pharmacopsychological study. The results of the psycho-experimental investigations are reported in this paper. The effects of oxazepam and/or ethanol doses given to 14 subjects with known blood concentrations of the respective substances were monitored and compared to placebo effects. - An analysis of the experiments with respect to the psychomotoric responses showed a tendency towards motoric instability under ethanol (A), whereas in the placebo experiment a relatively constant level of efficiency was maintained. In the oxazepam experiment (C) a slowing of the psychomotoric functions was observed. The concomitant influence of ethanol and oxazepam accentuates the motoric instability with a delay of the motoric responses. These effects remain visible, though slightly modified, after the administration of both substances at intervals (E). The experiments showed a decrease in efficiency during continuous exercise, a delayed response to optic and acoustic stimuli, and a retardation of the entire behavioral pattern under the influence of ethanol and oxazepam. The results indicate the existence of marked interactions between ethanol and oxazepam.", "PMID": 977201}
{"id": "pubmed23n0032_11013", "title": "Cytological effect of nogalamycin in mammals and regression of methylcholanthrene-induced and spontaneous tumors in rats.", "content": "The cytological effect of nogalamycin was studied in rats, mice and cultured human leukocytes. Four standard test systems were used: (1) Analysis of spermatogonial cells, (2) Cytological evaluation of bone-marrow metaphase plates, (3) micronucleus test in polychromatic erythrocytes, and (4) Determination of chromosomal aberrations in cultured human leukocytes. The results of the studies indicated that the types and frequencies of cytological errors induced by the drug were not significantly different from the concurrent controls. Chemically induced fibrosarcoma tumors and tumors of spontaneous origin in rats were successfully regressed following the administration of nogalamycin. Simultaneous transplantation of chemically induced fibrosarcoma tumors and intraperitoneal administration of nogalamycin, irreversibly inhibited growth of primary tumors in rats. The most effective concentration of nogalamycin for either regression of actively growing fibrosarcoma tumors or inhibition of transplanted fibrosarcoma cells was 556 mug/kg of body weight.", "contents": "Cytological effect of nogalamycin in mammals and regression of methylcholanthrene-induced and spontaneous tumors in rats. The cytological effect of nogalamycin was studied in rats, mice and cultured human leukocytes. Four standard test systems were used: (1) Analysis of spermatogonial cells, (2) Cytological evaluation of bone-marrow metaphase plates, (3) micronucleus test in polychromatic erythrocytes, and (4) Determination of chromosomal aberrations in cultured human leukocytes. The results of the studies indicated that the types and frequencies of cytological errors induced by the drug were not significantly different from the concurrent controls. Chemically induced fibrosarcoma tumors and tumors of spontaneous origin in rats were successfully regressed following the administration of nogalamycin. Simultaneous transplantation of chemically induced fibrosarcoma tumors and intraperitoneal administration of nogalamycin, irreversibly inhibited growth of primary tumors in rats. The most effective concentration of nogalamycin for either regression of actively growing fibrosarcoma tumors or inhibition of transplanted fibrosarcoma cells was 556 mug/kg of body weight.", "PMID": 977202}
{"id": "pubmed23n0032_11014", "title": "Photochemotherapy (PUVA) and psoriasis: comparison of 8-MOP and 8-MOP/5-MOP.", "content": "Twenty-eight psoriatic patients received PUVA treatment (psoralen and long ultraviolet irradiations. Two preparations were used; 8-methoxypsoralen and a mixture of 5-methoxypsoralen and 8-methoxypsoralen. Both gave considerable improvement, but in 6 cases the lesions reappeared after 2 to 8 weeks, in spite of maintenance treatment. In this report, photochemotherapy of psoriasis was compared, using a UVA emitting lamp, 8-MOP, and a mixture of 8-MOP and 5-methoxypsoralen (5-MOP).", "contents": "Photochemotherapy (PUVA) and psoriasis: comparison of 8-MOP and 8-MOP/5-MOP. Twenty-eight psoriatic patients received PUVA treatment (psoralen and long ultraviolet irradiations. Two preparations were used; 8-methoxypsoralen and a mixture of 5-methoxypsoralen and 8-methoxypsoralen. Both gave considerable improvement, but in 6 cases the lesions reappeared after 2 to 8 weeks, in spite of maintenance treatment. In this report, photochemotherapy of psoriasis was compared, using a UVA emitting lamp, 8-MOP, and a mixture of 8-MOP and 5-methoxypsoralen (5-MOP).", "PMID": 977210}
{"id": "pubmed23n0032_11015", "title": "Trimethoxypsoralen in vitiligo.", "content": "The treatment of vitiligo has been unsatisfactory. Introduction of systemic furocoumarins to treatment regimens has given very promising results. Trimethylpsoralen has been found to be more potent than 8-methoxypsoralen. In cases studied, ultraviolet irradiation of affected sites following topical application of trimethylpsoralen was found to hasten the process of repigmentation. Simultaneous treatment of underlying disease produced a quicker response followed by sustained relief.", "contents": "Trimethoxypsoralen in vitiligo. The treatment of vitiligo has been unsatisfactory. Introduction of systemic furocoumarins to treatment regimens has given very promising results. Trimethylpsoralen has been found to be more potent than 8-methoxypsoralen. In cases studied, ultraviolet irradiation of affected sites following topical application of trimethylpsoralen was found to hasten the process of repigmentation. Simultaneous treatment of underlying disease produced a quicker response followed by sustained relief.", "PMID": 977211}
{"id": "pubmed23n0032_11016", "title": "Leucocyte ascorbic acid in abnormal leucocyte states.", "content": "Leucocyte ascorbic acid was estimated in patients with abnormal leucocyte states and other haematological disorders. Levels below the normal range were found in most cases of chronic myeloid leukaemia and chronic lymphatic leukaemia. Subnormal levels were found in more than a third of patients with acute leukaemias, lymphomas, glandular fever, myelofibrosis, and polycythaemia, and in the same proportion of patients receiving cytotoxic drugs, and also in those with a polymorph leucocytosis and those with purpura. Most patients with anaemia but a normal leucocyte count, and those with myelomatosis had normal levels. The majority of pregnant women tested had subnormal levels. In a wide variety of leucocyte disorders the leucocyte ascorbic acid may not be an accurate index of the body's Vitamin C status. The results also support the supposition that leucocyte ascorbic acid is mostly carried by normal mature polymorphs.", "contents": "Leucocyte ascorbic acid in abnormal leucocyte states. Leucocyte ascorbic acid was estimated in patients with abnormal leucocyte states and other haematological disorders. Levels below the normal range were found in most cases of chronic myeloid leukaemia and chronic lymphatic leukaemia. Subnormal levels were found in more than a third of patients with acute leukaemias, lymphomas, glandular fever, myelofibrosis, and polycythaemia, and in the same proportion of patients receiving cytotoxic drugs, and also in those with a polymorph leucocytosis and those with purpura. Most patients with anaemia but a normal leucocyte count, and those with myelomatosis had normal levels. The majority of pregnant women tested had subnormal levels. In a wide variety of leucocyte disorders the leucocyte ascorbic acid may not be an accurate index of the body's Vitamin C status. The results also support the supposition that leucocyte ascorbic acid is mostly carried by normal mature polymorphs.", "PMID": 977212}
{"id": "pubmed23n0032_11017", "title": "L-ascorbic acid, L-ascorbate 2-sulfate, and atherogenesis.", "content": "Rabbits on a high cholesterol diet were divided into three groups: one group received subcutaneous injections of physiological saline 3 times/day, 5 days/wk for 10 wk; another group received subcutaneous injections of L-ascorbic acid (0.37 mmole) according to the same timetable; and the third group was administered an equivalent amount of L-ascorbate 2-sulfate as outlined above. Each week the serum levels of total and free cholesterol and triglycerides were measured. At the end of 10 wk the animals were killed and the cholesterol content of the livers, spleens, and adrenal glands was measured. The aortas were examined for plaque deposition; the deposits were excised and pooled according to groups; and the total mass and cholesterol contents of the pooled plaques were determined. Administration of ascorbic acid or ascorbate 2-sulfate did not prevent hypercholesterolemia or elevated levels of serum triglycerides. No significant differences among the groups were found either in tissue cholesterol levels or in the extent or type of lesions found. Although plaque deposition appeared to be similar in the aortas of these animals, a marked difference was found in total mass and cholesterol content of the plaques: The plaques of the saline-treated group had a total mass and cholesterol content approximately 2.5 times that found in the group injected with ascorbic acid and about 1.5 times that found in the animals treated with ascorbate 2-sulfate. These results indicate that ascorbic acid, in particular, minimizes the total quantity of plaque deposition even though it is ineffective in preventing hypercholesterolemia, elevated serum triglycerides, and accumulation of cholesterol by several tissues.", "contents": "L-ascorbic acid, L-ascorbate 2-sulfate, and atherogenesis. Rabbits on a high cholesterol diet were divided into three groups: one group received subcutaneous injections of physiological saline 3 times/day, 5 days/wk for 10 wk; another group received subcutaneous injections of L-ascorbic acid (0.37 mmole) according to the same timetable; and the third group was administered an equivalent amount of L-ascorbate 2-sulfate as outlined above. Each week the serum levels of total and free cholesterol and triglycerides were measured. At the end of 10 wk the animals were killed and the cholesterol content of the livers, spleens, and adrenal glands was measured. The aortas were examined for plaque deposition; the deposits were excised and pooled according to groups; and the total mass and cholesterol contents of the pooled plaques were determined. Administration of ascorbic acid or ascorbate 2-sulfate did not prevent hypercholesterolemia or elevated levels of serum triglycerides. No significant differences among the groups were found either in tissue cholesterol levels or in the extent or type of lesions found. Although plaque deposition appeared to be similar in the aortas of these animals, a marked difference was found in total mass and cholesterol content of the plaques: The plaques of the saline-treated group had a total mass and cholesterol content approximately 2.5 times that found in the group injected with ascorbic acid and about 1.5 times that found in the animals treated with ascorbate 2-sulfate. These results indicate that ascorbic acid, in particular, minimizes the total quantity of plaque deposition even though it is ineffective in preventing hypercholesterolemia, elevated serum triglycerides, and accumulation of cholesterol by several tissues.", "PMID": 977213}
{"id": "pubmed23n0032_11018", "title": "Effects of L-lysine administration on certain aspects of ascorbic acid metabolism.", "content": "The effects of administration of L-lysine on total ascorbic acid level of various tissues and plasma of rats were studied. The biosynthesis of L-ascorbic acid by the liver tissue was also followed. L-lysine was administered at a dose of 88.3 mg day-1 (20% of LD50) for each 100 g body weight for 14 days. L-lysine administration at the present dose elevated the total ascorbic acid level of liver, kidney, testes, spleen and brain tissues. The plasma total ascorbic acid level was also elevated. The synthesis of L-ascorbic acid from both D-glucuronolactone and L-gulonolactone by the liver was, however, reduced after L-lysine administration. It has been suggested that L-lysine administration at the present dose altered the plasma amino acid pattern which in turn impaired the in vivo synthesis of tissue proteins and, consequently, the synthesis of apoproteins of ascorbic acid-synthesizing enzymes, the D-glucuronoreductase and L-gulonooxidase, were reduced. The elevation in the total ascorbic acid level of extra-hepatic tissues and plasma after L-lysine administration was ascribed to the reduced catabolism and diminished urinary excretion of ascorbic acid.", "contents": "Effects of L-lysine administration on certain aspects of ascorbic acid metabolism. The effects of administration of L-lysine on total ascorbic acid level of various tissues and plasma of rats were studied. The biosynthesis of L-ascorbic acid by the liver tissue was also followed. L-lysine was administered at a dose of 88.3 mg day-1 (20% of LD50) for each 100 g body weight for 14 days. L-lysine administration at the present dose elevated the total ascorbic acid level of liver, kidney, testes, spleen and brain tissues. The plasma total ascorbic acid level was also elevated. The synthesis of L-ascorbic acid from both D-glucuronolactone and L-gulonolactone by the liver was, however, reduced after L-lysine administration. It has been suggested that L-lysine administration at the present dose altered the plasma amino acid pattern which in turn impaired the in vivo synthesis of tissue proteins and, consequently, the synthesis of apoproteins of ascorbic acid-synthesizing enzymes, the D-glucuronoreductase and L-gulonooxidase, were reduced. The elevation in the total ascorbic acid level of extra-hepatic tissues and plasma after L-lysine administration was ascribed to the reduced catabolism and diminished urinary excretion of ascorbic acid.", "PMID": 977214}
{"id": "pubmed23n0032_11019", "title": "Cardiac and renal pentose phosphate pathway activity in thiamine deficiency.", "content": "Thiamine deficiency was produced in young rats by feeding a thiamine deficient diet. At a time when neurological symptoms were severe, and cardiac and renal transketolase activities were decreased, the animals were sacrificed. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities, and flux through the pentose phosphate pathway were similar in pair-fed control and thiamine deficient rats. These data suggest that altered pentose phosphate pathway activity is not a vital feature of murine thiamine deficiency.", "contents": "Cardiac and renal pentose phosphate pathway activity in thiamine deficiency. Thiamine deficiency was produced in young rats by feeding a thiamine deficient diet. At a time when neurological symptoms were severe, and cardiac and renal transketolase activities were decreased, the animals were sacrificed. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities, and flux through the pentose phosphate pathway were similar in pair-fed control and thiamine deficient rats. These data suggest that altered pentose phosphate pathway activity is not a vital feature of murine thiamine deficiency.", "PMID": 977215}
{"id": "pubmed23n0032_11020", "title": "Liver and brain thiamin depletion and neurologic signs in pigeon athiaminosis.", "content": "Blood pyruvate and free and phosphorylated thiamin contents of liver and brain were measured in pigeons during diatary thiamin deficiency. Neurologic signs (opisthotonus and ataxia) appeared in the course of thiamin deficiency or did not appear at all during the entire athiaminosis period. As compared to respective controls, which never exhibited any neurologic abnormalities in spite of thiamin deficiency, the symptomatic animals had a higher pyruvate level in blood and a significantly lower phosphorylated thiamin content in both liver and brain. A hypothesis on relationship between phosphorylated thiamin content of liver and brain and occurence of nervous symptoms was expressed.", "contents": "Liver and brain thiamin depletion and neurologic signs in pigeon athiaminosis. Blood pyruvate and free and phosphorylated thiamin contents of liver and brain were measured in pigeons during diatary thiamin deficiency. Neurologic signs (opisthotonus and ataxia) appeared in the course of thiamin deficiency or did not appear at all during the entire athiaminosis period. As compared to respective controls, which never exhibited any neurologic abnormalities in spite of thiamin deficiency, the symptomatic animals had a higher pyruvate level in blood and a significantly lower phosphorylated thiamin content in both liver and brain. A hypothesis on relationship between phosphorylated thiamin content of liver and brain and occurence of nervous symptoms was expressed.", "PMID": 977216}
{"id": "pubmed23n0032_11021", "title": "Biotin deficiency in chicks fed a wheat-based diet.", "content": "A wheat-based diet produced severe biotin deficiency symptoms appearing at the age of ten to fourteen days and becoming very severe in the third and fourth week (group 1). Biotin supplementation with 50 mug/kg (group 2) reduced the symptoms almost completely, but did not restore completely growth compared to chicks receiving the diet supplemented with 300 mug biotin/kg compared to chicks receiving the diet supplemented with 300 mug biotin/kg (group 3). The plasma level of biotin was about, or lower than, 100 ng/100 ml plasma in groups 1 and 2, indicating biotin deficiency. In group 3, plasma biotin was above 200 ng/100 ml. Liver biotin, after two weeks, was low in group 1 (less than 600 ng/g), medium in group 2 (1000 to 1500 ng/g) and in group 3 above 2000 ng/g. Plasma and liver biotin levels are found to be suitable parameters for diagnosis of subclinical biotin deficiency in chicks.", "contents": "Biotin deficiency in chicks fed a wheat-based diet. A wheat-based diet produced severe biotin deficiency symptoms appearing at the age of ten to fourteen days and becoming very severe in the third and fourth week (group 1). Biotin supplementation with 50 mug/kg (group 2) reduced the symptoms almost completely, but did not restore completely growth compared to chicks receiving the diet supplemented with 300 mug biotin/kg compared to chicks receiving the diet supplemented with 300 mug biotin/kg (group 3). The plasma level of biotin was about, or lower than, 100 ng/100 ml plasma in groups 1 and 2, indicating biotin deficiency. In group 3, plasma biotin was above 200 ng/100 ml. Liver biotin, after two weeks, was low in group 1 (less than 600 ng/g), medium in group 2 (1000 to 1500 ng/g) and in group 3 above 2000 ng/g. Plasma and liver biotin levels are found to be suitable parameters for diagnosis of subclinical biotin deficiency in chicks.", "PMID": 977217}
{"id": "pubmed23n0032_11022", "title": "[Effects of a high dose of vitamin A on experimental immunologic arthritis].", "content": "Vitamin A, a good adjuvant to immunologic reactions, has in this aspect an interesting therapeutic effect, especially in immunotherapy. On the other hand, in diseases caused by circulating immunocomplexes or in auto-immune diseases, vitamin A can prolong the pathologic immunologic process. This experimental study shows that vitamin A in high doses has an adjuvant effect, that is aggravating considerably the immunologic arthritis induced in the Wistar rat.", "contents": "[Effects of a high dose of vitamin A on experimental immunologic arthritis]. Vitamin A, a good adjuvant to immunologic reactions, has in this aspect an interesting therapeutic effect, especially in immunotherapy. On the other hand, in diseases caused by circulating immunocomplexes or in auto-immune diseases, vitamin A can prolong the pathologic immunologic process. This experimental study shows that vitamin A in high doses has an adjuvant effect, that is aggravating considerably the immunologic arthritis induced in the Wistar rat.", "PMID": 977218}
{"id": "pubmed23n0032_11023", "title": "Retinyl palmitate, a major retinyl ester in rat testis seminiferous tubules. II. The Sertoli cell, site of retinol esterification.", "content": "The study was designed to 1) isolate and quantitate retinyl esters normally present in the testis seminiferous tubules (ST) and 2) determine whether the Sertoli (SE) cells in the ST have the enzyme system necessary to esterify retinol. In the first study testes from normal adult male rats were removed, the ST were isolated mechanically and retinol was extracted and separated into free and retinyl esters in a prestandardized alumina column. The fraction containing retinyl esters was hydrolyzed, and the fatty acid composition was determined gas chromatographically. The results showed that retinyl palmitate was the major retinyl ester in the testis ST constituting almost 55% of the total esters. In the second study adult male rats were given Busulfan (1,4, dimethylsulfonoxybutane) intraperitoneally twice six weeks apart. At the end of six weeks following injection, the testes from a few animals were examined histologically, and the results showed that Busulfan treatment destroyed the germinal epithelium completely but had no effect on the structural integrity of the SE cells. Labeled retinol (free) mixed in rat albumin solution was injected intratesticularly (in vivo study) or incubated (in vitro study) with ST which had been isolated from the testes of rats treated with Busulfan. The results of the studies indicate that labeled retinol (free) was converted to retinyl esters in the ST. Since the ratio of conversion of free retinol (labeled) to retinyl esters remained constant in the treated ST (which contained only Sertoli cells, the germinal epithelium having been destroyed) and in the control ST (which contained both Sertoli cells and germinal epithelium), we conclude that the Sertoli cells are the site of retinol esterification in the testis ST.", "contents": "Retinyl palmitate, a major retinyl ester in rat testis seminiferous tubules. II. The Sertoli cell, site of retinol esterification. The study was designed to 1) isolate and quantitate retinyl esters normally present in the testis seminiferous tubules (ST) and 2) determine whether the Sertoli (SE) cells in the ST have the enzyme system necessary to esterify retinol. In the first study testes from normal adult male rats were removed, the ST were isolated mechanically and retinol was extracted and separated into free and retinyl esters in a prestandardized alumina column. The fraction containing retinyl esters was hydrolyzed, and the fatty acid composition was determined gas chromatographically. The results showed that retinyl palmitate was the major retinyl ester in the testis ST constituting almost 55% of the total esters. In the second study adult male rats were given Busulfan (1,4, dimethylsulfonoxybutane) intraperitoneally twice six weeks apart. At the end of six weeks following injection, the testes from a few animals were examined histologically, and the results showed that Busulfan treatment destroyed the germinal epithelium completely but had no effect on the structural integrity of the SE cells. Labeled retinol (free) mixed in rat albumin solution was injected intratesticularly (in vivo study) or incubated (in vitro study) with ST which had been isolated from the testes of rats treated with Busulfan. The results of the studies indicate that labeled retinol (free) was converted to retinyl esters in the ST. Since the ratio of conversion of free retinol (labeled) to retinyl esters remained constant in the treated ST (which contained only Sertoli cells, the germinal epithelium having been destroyed) and in the control ST (which contained both Sertoli cells and germinal epithelium), we conclude that the Sertoli cells are the site of retinol esterification in the testis ST.", "PMID": 977219}
{"id": "pubmed23n0032_11024", "title": "Regulatory action of phenylbenzo-gamma-pyrone (PBP) derivatives on blood constituents affecting rheology in patients with coronary heart disease (CHD).", "content": "In patients with CHD the hematological and serum chemical profiles showed significantly elevated (P less than 0.05) serum cholesterols, highly significantly increased hematocrits (P less than 0.025 to P less than 0.001) and the erythrocyte sedimentation rates (ESR) (red cell aggregation) along with several blood constituents causal of erythrocyte aggregation were significantly elevated (P less than 0.05 to P less than 0.025). Thus, major defects were elevated serum cholesterol, increased blood viscosity and reduced tissue perfusion which present evidence implicates as interactive factors in the pathogenesis of CHD. Methoxylated PBP derivatives exhibited a highly significant antiadhesive action (P less than 0.001) on erythrocytes and certain PBP hydroxylated glycosides showed a significant accelerating (P less than 0.05) action on erythrocyte aggregation which causes sequestration and reduced erythrocyte concentration. Thus, the PBP derivatives exert an apparent regulatory action on erythrocyte aggregation and concentration, two major factors affecting blood viscosity and flow. The PBP compounds occur in plants and are found in certain foods which suggests dietary control of the blood high viscosity syndrome.", "contents": "Regulatory action of phenylbenzo-gamma-pyrone (PBP) derivatives on blood constituents affecting rheology in patients with coronary heart disease (CHD). In patients with CHD the hematological and serum chemical profiles showed significantly elevated (P less than 0.05) serum cholesterols, highly significantly increased hematocrits (P less than 0.025 to P less than 0.001) and the erythrocyte sedimentation rates (ESR) (red cell aggregation) along with several blood constituents causal of erythrocyte aggregation were significantly elevated (P less than 0.05 to P less than 0.025). Thus, major defects were elevated serum cholesterol, increased blood viscosity and reduced tissue perfusion which present evidence implicates as interactive factors in the pathogenesis of CHD. Methoxylated PBP derivatives exhibited a highly significant antiadhesive action (P less than 0.001) on erythrocytes and certain PBP hydroxylated glycosides showed a significant accelerating (P less than 0.05) action on erythrocyte aggregation which causes sequestration and reduced erythrocyte concentration. Thus, the PBP derivatives exert an apparent regulatory action on erythrocyte aggregation and concentration, two major factors affecting blood viscosity and flow. The PBP compounds occur in plants and are found in certain foods which suggests dietary control of the blood high viscosity syndrome.", "PMID": 977220}
{"id": "pubmed23n0032_11025", "title": "The effect of non-protein food constituents on the nutritive value of radiation-sterilized casein.", "content": "The effect of radiation sterilization on casein alone and in the presence of glucose or starch was assessed by means of nitrogen balance studies in growing rats and compared to the effect of heat sterilization. No decrease in protein digestibility and utilization was noticed in the irradiated samples nor did the presence of glucose or starch during processing cause and changes of these parameters. Following heat sterilization of casein in the presence of glucose there was a significant reduction in protein digestibility and Net Protein Utilization (NPU). These changes were accompanied by a drastic decline of available lysine. The inclusion of 3% agar-agar (aqueous solution) in the diet induced a drastic rise in endogenous faecal nitrogen losses and a corresponding decrease in apparent protein digestibility and NPU.", "contents": "The effect of non-protein food constituents on the nutritive value of radiation-sterilized casein. The effect of radiation sterilization on casein alone and in the presence of glucose or starch was assessed by means of nitrogen balance studies in growing rats and compared to the effect of heat sterilization. No decrease in protein digestibility and utilization was noticed in the irradiated samples nor did the presence of glucose or starch during processing cause and changes of these parameters. Following heat sterilization of casein in the presence of glucose there was a significant reduction in protein digestibility and Net Protein Utilization (NPU). These changes were accompanied by a drastic decline of available lysine. The inclusion of 3% agar-agar (aqueous solution) in the diet induced a drastic rise in endogenous faecal nitrogen losses and a corresponding decrease in apparent protein digestibility and NPU.", "PMID": 977221}
{"id": "pubmed23n0032_11026", "title": "Interactions between malnutrition and primaquine studied on Wistar rats. II. Complex vitamin B deficiency and thiamine deficiency.", "content": "The action of primaquine was investigated on male Wistar rats depleted on the vitamin B complex (approximately 50% of their requirement for optimal growth), on thiamine (approximately 50% of their requirement for optimal growth), and pair-fed control animals. There was only a marginal increase in adverse primaquine reactions in the malnourished, especially in the thiamine deficient rats.", "contents": "Interactions between malnutrition and primaquine studied on Wistar rats. II. Complex vitamin B deficiency and thiamine deficiency. The action of primaquine was investigated on male Wistar rats depleted on the vitamin B complex (approximately 50% of their requirement for optimal growth), on thiamine (approximately 50% of their requirement for optimal growth), and pair-fed control animals. There was only a marginal increase in adverse primaquine reactions in the malnourished, especially in the thiamine deficient rats.", "PMID": 977222}
{"id": "pubmed23n0032_11027", "title": "In Trinidad: angular stomatitis and pregnancy.", "content": "Riboflavin deficiency is endemic in Trinidad. A syndrome is observed peculiar to pregnancy. It occurs in the second trimester in women with low blood riboflavin levels. Treatment with riboflavin gives clinical improvement in mothers and raises fetal blood levels. Early biochemical diagnosis and treatment of subclinical deficiency is of value to the Trinidadian mother but protection to the fetus is doubtful. This is because of the effect of multiple limiting factors in which the mother is deficient.", "contents": "In Trinidad: angular stomatitis and pregnancy. Riboflavin deficiency is endemic in Trinidad. A syndrome is observed peculiar to pregnancy. It occurs in the second trimester in women with low blood riboflavin levels. Treatment with riboflavin gives clinical improvement in mothers and raises fetal blood levels. Early biochemical diagnosis and treatment of subclinical deficiency is of value to the Trinidadian mother but protection to the fetus is doubtful. This is because of the effect of multiple limiting factors in which the mother is deficient.", "PMID": 977223}
{"id": "pubmed23n0032_11028", "title": "The effect of hypervitaminosis A on the sialic acid and hexosamine contents of the salivary glands of rats.", "content": "The effect of orally feeding 15000 I.U. of vitamin A during 15 days to male rats of the Wistar strain was studied on the sialic acid and hexosamine contents of the salivary glands. Sialic acid and hexosamine contents in the submandibular and sublingual salivary glands showed a decrease while those for the parotid salivary gland did not alter.", "contents": "The effect of hypervitaminosis A on the sialic acid and hexosamine contents of the salivary glands of rats. The effect of orally feeding 15000 I.U. of vitamin A during 15 days to male rats of the Wistar strain was studied on the sialic acid and hexosamine contents of the salivary glands. Sialic acid and hexosamine contents in the submandibular and sublingual salivary glands showed a decrease while those for the parotid salivary gland did not alter.", "PMID": 977224}
{"id": "pubmed23n0032_11029", "title": "Effect of varying vitamin B6 intake of early-weaned piglets on urinary xanthurenic and kynurenic acid excretion, serum transaminase activity and urea concentration.", "content": "In a bipartite rearing experiment (day 1-24 and 24-45) 72 early-weaned piglets were used to study the effect of varying dietary vitamin B6 contents on renal excretion of xanthurenic and kynurenic acid after a tryptophan load, on urea concentration and activities of two transaminases in serum at the end of each period. The animals, divided into 6 groups, were fed ad libitum a prestarter and a starter in period I and II, respectively, each containing 0.5, 1.2, 2.0, 2.8, 3.5 or 6.6 mg vitamin B6 per kg dry matter. The urinary xanthurenic acid excretion was elevated especially at the vitamin B6 supply of 0.5 ppm and rose severalfold with increasing depletion time (period II). In both periods, the smallest amount was excreted by piglets supplemented with 2.8 ppm. In comparison to groups B (1.2 ppm) and C (2.0 ppm), their average excretion rate was reduced by 29% and 15%, respectively, in period I and by 50% and 22%, respectively, in period II. Analogously to xanthurenic acid, the smallest amount of kynurenic acid was excreted by group D (2.8 ppm). Starting from the lowest vitamin B6 supply, the activity of SGPT showed an almost linear increase in both experimental periods. In contrast, SGOT already reached an upper activity level with the dietary vitamin B6 content of 3.5 and 2.8 ppm at the end of period I and II, respectively. The concentration of serum urea was influenced only by the lowest vitamin B6 supply of 0.5 ppm.", "contents": "Effect of varying vitamin B6 intake of early-weaned piglets on urinary xanthurenic and kynurenic acid excretion, serum transaminase activity and urea concentration. In a bipartite rearing experiment (day 1-24 and 24-45) 72 early-weaned piglets were used to study the effect of varying dietary vitamin B6 contents on renal excretion of xanthurenic and kynurenic acid after a tryptophan load, on urea concentration and activities of two transaminases in serum at the end of each period. The animals, divided into 6 groups, were fed ad libitum a prestarter and a starter in period I and II, respectively, each containing 0.5, 1.2, 2.0, 2.8, 3.5 or 6.6 mg vitamin B6 per kg dry matter. The urinary xanthurenic acid excretion was elevated especially at the vitamin B6 supply of 0.5 ppm and rose severalfold with increasing depletion time (period II). In both periods, the smallest amount was excreted by piglets supplemented with 2.8 ppm. In comparison to groups B (1.2 ppm) and C (2.0 ppm), their average excretion rate was reduced by 29% and 15%, respectively, in period I and by 50% and 22%, respectively, in period II. Analogously to xanthurenic acid, the smallest amount of kynurenic acid was excreted by group D (2.8 ppm). Starting from the lowest vitamin B6 supply, the activity of SGPT showed an almost linear increase in both experimental periods. In contrast, SGOT already reached an upper activity level with the dietary vitamin B6 content of 3.5 and 2.8 ppm at the end of period I and II, respectively. The concentration of serum urea was influenced only by the lowest vitamin B6 supply of 0.5 ppm.", "PMID": 977225}
{"id": "pubmed23n0032_11030", "title": "Effect of hyper- and hypothyroidism on the alpha-tocopherol concentration in serum and some organs of growing rats.", "content": "The finding of a raised alpha-tocopherol concentration in the heart muscle, liver, skeletal muscle and serum during experimental hyperthyroidism, lasting 30 days, made the authors assume that this cumulation is a transient phenomenon which would disappear during a prolonged metabolic disorder as a result of energetic breakdown of the organism. The purpose of the paper was to verify this view. The investigation with experimental hyper- and hypothyroidism was made in 120 rats with a mean weight of 160 g. To 60 animals daily 100 mug thyroxin were administered by the i.p. route, 48 animals were given 2.5 Carbimazol daily by mouth to induce hypothyroidism and 12 animals served as controls. The animals were fed the same diet. The effect of the administered preparations was examined after 20, 30, 40, 50 and 60 days. In hypothyroid animals the 50th day was omitted. The results revealed: 1. In hyperthyroid animals the body weight was almost constant to the end of the experiment. However these animals had a significantly raised weight of the heart muscle and liver, as compared with the remaining two groups. 2. In hyperthyroid animals the highest alpha-tocopherol concentration in the heart muscle and skeletal muscle was recorded after 20 days. During subsequent time intervals the alpha-tocopherol concentration in the heart muscle declined gradually. In the skeletal muscle a dramatic decline occurred after 40 days and proceeded to the end of the investigation when the recorded values were at the borderline of detectability. This finding confirmed the authors view that the alpha-tocopherol cumulation in muscle exposed to an increased work load is a transient phenomenon and disappears during prolonged hyperthyroidism before the breakdown of the organism. 3. In hyperthyroidism no correlation was found between the alpha-tocopherol concentration and the lipid content of organs and skeletal muscle. This correlation was found in hypothyroidism. 4. The alpha-tocopherol serum level in hyperthyroidism was significantly higher than in hypothyroidism. 5. In hyperthyroidism the alpha-tocopherol cumulation in liver increased gradually, and reached its maximum after 50 days. At a time when the alpha-tocopherol concentration in the heart muscle and skeletal muscle declined already, its liver concentration increased. This phenomenon may be associated with the increasing protein synthesis in the liver during hyperthyroidism, or with other phenomena which call for further investigation.", "contents": "Effect of hyper- and hypothyroidism on the alpha-tocopherol concentration in serum and some organs of growing rats. The finding of a raised alpha-tocopherol concentration in the heart muscle, liver, skeletal muscle and serum during experimental hyperthyroidism, lasting 30 days, made the authors assume that this cumulation is a transient phenomenon which would disappear during a prolonged metabolic disorder as a result of energetic breakdown of the organism. The purpose of the paper was to verify this view. The investigation with experimental hyper- and hypothyroidism was made in 120 rats with a mean weight of 160 g. To 60 animals daily 100 mug thyroxin were administered by the i.p. route, 48 animals were given 2.5 Carbimazol daily by mouth to induce hypothyroidism and 12 animals served as controls. The animals were fed the same diet. The effect of the administered preparations was examined after 20, 30, 40, 50 and 60 days. In hypothyroid animals the 50th day was omitted. The results revealed: 1. In hyperthyroid animals the body weight was almost constant to the end of the experiment. However these animals had a significantly raised weight of the heart muscle and liver, as compared with the remaining two groups. 2. In hyperthyroid animals the highest alpha-tocopherol concentration in the heart muscle and skeletal muscle was recorded after 20 days. During subsequent time intervals the alpha-tocopherol concentration in the heart muscle declined gradually. In the skeletal muscle a dramatic decline occurred after 40 days and proceeded to the end of the investigation when the recorded values were at the borderline of detectability. This finding confirmed the authors view that the alpha-tocopherol cumulation in muscle exposed to an increased work load is a transient phenomenon and disappears during prolonged hyperthyroidism before the breakdown of the organism. 3. In hyperthyroidism no correlation was found between the alpha-tocopherol concentration and the lipid content of organs and skeletal muscle. This correlation was found in hypothyroidism. 4. The alpha-tocopherol serum level in hyperthyroidism was significantly higher than in hypothyroidism. 5. In hyperthyroidism the alpha-tocopherol cumulation in liver increased gradually, and reached its maximum after 50 days. At a time when the alpha-tocopherol concentration in the heart muscle and skeletal muscle declined already, its liver concentration increased. This phenomenon may be associated with the increasing protein synthesis in the liver during hyperthyroidism, or with other phenomena which call for further investigation.", "PMID": 977226}
{"id": "pubmed23n0032_11031", "title": "Urea cycle enzyme activity in lambs fed different nitrogen levels at low dietary intakes.", "content": "Eleven Southdown male lambs averaging 19.8 kg were randomly allotted to two groups and fed diets containing 7.7% (low-N) or 15.8% (high-N) crude protein. All of the supplemental nitrogen in the high-N diet was supplied as urea. Intake of the low-N and high-N diets averaged 372.3 g and 340.5 g/day, respectively. Findings at the end of the thirty-day trial were: (1) mean body weights unchanged for the two groups; (2) plasma urea nitrogen three-fold higher in the high-N (19.07 mg/100 ml) than the low-N (6.57) animals; (3) similar hepatic activity levels of three urea cycle enzymes (ornithine transcarbamylase, argininosuccinase, arginase) in the two groups, and (4) similar liver weights and liver protein concentration. The absence of adaptive change in enzyme levels suggests the hypothesis that addition of non-protein nitrogen to maintenance diets may cause ammonia intoxication by exceeding the liver's reserve capacity for urea synthesis.", "contents": "Urea cycle enzyme activity in lambs fed different nitrogen levels at low dietary intakes. Eleven Southdown male lambs averaging 19.8 kg were randomly allotted to two groups and fed diets containing 7.7% (low-N) or 15.8% (high-N) crude protein. All of the supplemental nitrogen in the high-N diet was supplied as urea. Intake of the low-N and high-N diets averaged 372.3 g and 340.5 g/day, respectively. Findings at the end of the thirty-day trial were: (1) mean body weights unchanged for the two groups; (2) plasma urea nitrogen three-fold higher in the high-N (19.07 mg/100 ml) than the low-N (6.57) animals; (3) similar hepatic activity levels of three urea cycle enzymes (ornithine transcarbamylase, argininosuccinase, arginase) in the two groups, and (4) similar liver weights and liver protein concentration. The absence of adaptive change in enzyme levels suggests the hypothesis that addition of non-protein nitrogen to maintenance diets may cause ammonia intoxication by exceeding the liver's reserve capacity for urea synthesis.", "PMID": 977228}
{"id": "pubmed23n0032_11032", "title": "Public health significance of zoonotic tapeworms in Korea.", "content": "Through an epidemiological review on the zoonotic tapeworms in Korea, the frequency and severity of the zoonoses have been recognized. Taeniasis and human cysticercosis are of importance to the public health in Korea. The frequency of taeniasis is 0.3% to 12.7% discovered by stool examination and 4.5% to 38.0% discovered by questionaire survey. Taeniasis occurs more frequently in males than in females and, especially, in both sexes in the age-group of 20 to 49. T. saginata is more common that T. solium. No case of human cysticercosis caused by T.saginata has been reported in Korea. To the contrary, human cysticercosis caused by T. solium has been reported frequently during the 1960's. The severity of human cysticercosis is a significant problem of public health in Korea. Old data on bovine cysticercosis in the 1920's and 1930's are not useful for present control measures. Systematic surveys on bovine and swine cysticercosis as well as taeniasis and human cysticercosis are needed in Korea. Cases of sparganosis have been reported frequently during the past 15 years. Most of the 34 cases of sparganosis reported involved the eating of raw snakes and frogs. Most of the cases occurred in older males. Sparganosis in snakes, frogs, chickens, and swine has been reported in Korea. Human infection of Hymenolepis nana ranged from 0.2% to 1.4% discovered by stool examination. A few cases of adult worm collections of Diphyllobothrium latum and Hymenolepis diminuta have been reported in Korea. Two cases of human hydatid disease have been reported in Korea. No study on the disease in domestic animals is available. No case of human infection with dog tapeworm has been reported, even though it is highly prevalent in the indigenous dogs in Korea. I recommend that further study on the zoonotic tapeworms be conducted epidemiologically in Korea to get basic data for the public health programming.", "contents": "Public health significance of zoonotic tapeworms in Korea. Through an epidemiological review on the zoonotic tapeworms in Korea, the frequency and severity of the zoonoses have been recognized. Taeniasis and human cysticercosis are of importance to the public health in Korea. The frequency of taeniasis is 0.3% to 12.7% discovered by stool examination and 4.5% to 38.0% discovered by questionaire survey. Taeniasis occurs more frequently in males than in females and, especially, in both sexes in the age-group of 20 to 49. T. saginata is more common that T. solium. No case of human cysticercosis caused by T.saginata has been reported in Korea. To the contrary, human cysticercosis caused by T. solium has been reported frequently during the 1960's. The severity of human cysticercosis is a significant problem of public health in Korea. Old data on bovine cysticercosis in the 1920's and 1930's are not useful for present control measures. Systematic surveys on bovine and swine cysticercosis as well as taeniasis and human cysticercosis are needed in Korea. Cases of sparganosis have been reported frequently during the past 15 years. Most of the 34 cases of sparganosis reported involved the eating of raw snakes and frogs. Most of the cases occurred in older males. Sparganosis in snakes, frogs, chickens, and swine has been reported in Korea. Human infection of Hymenolepis nana ranged from 0.2% to 1.4% discovered by stool examination. A few cases of adult worm collections of Diphyllobothrium latum and Hymenolepis diminuta have been reported in Korea. Two cases of human hydatid disease have been reported in Korea. No study on the disease in domestic animals is available. No case of human infection with dog tapeworm has been reported, even though it is highly prevalent in the indigenous dogs in Korea. I recommend that further study on the zoonotic tapeworms be conducted epidemiologically in Korea to get basic data for the public health programming.", "PMID": 977229}
{"id": "pubmed23n0032_11033", "title": "Studies on morphological characteristics of Japanese strain of Schistosoma japonicum in rabbits.", "content": "The present investigation was undertaken to determine some morphological characteristics of Japanese strain of Schistosoma japonicum, the results are summarized as follows: (1) The pattern of testes in male worms can be divided into two types: the tandem (37.6%) and non-tandem (62.4%). (2) The number of testes varied from 1 to 13. The percentage of specimens with 7 testes was much higher than of others with both in tandem (70% against 30%) and non-tandem (86% against 14%) arrangement. (3) The unusual/irregular sets of testes including 2-3 additional sets were found in 158 (or 4.5%) of 3,498 male worms. Specimens with 7 testes in the unusual/irregular location numbered the highest (69.6%); the next with 8 (14.6%) and 6 (12%); and the lowest with 5 (3.8%). (4) Forty or 2.8% of 1,417 male worms were found to have 2-4 cecal loops. The cecal union located in 71-80% of worm bodys from the anterior end of male worm was found in highest number; the next in 61-70%; and the lowest in 51-60% and 81-90%. The location of united ceca in female worms was found all in the posterior part of body. (5) The ovary in all 125 females was located in the posterior portion of worm body except in 4 specimens. (6) The number of eggs in the uterus of female worms varied from 50 to 300. The mean number was 103.", "contents": "Studies on morphological characteristics of Japanese strain of Schistosoma japonicum in rabbits. The present investigation was undertaken to determine some morphological characteristics of Japanese strain of Schistosoma japonicum, the results are summarized as follows: (1) The pattern of testes in male worms can be divided into two types: the tandem (37.6%) and non-tandem (62.4%). (2) The number of testes varied from 1 to 13. The percentage of specimens with 7 testes was much higher than of others with both in tandem (70% against 30%) and non-tandem (86% against 14%) arrangement. (3) The unusual/irregular sets of testes including 2-3 additional sets were found in 158 (or 4.5%) of 3,498 male worms. Specimens with 7 testes in the unusual/irregular location numbered the highest (69.6%); the next with 8 (14.6%) and 6 (12%); and the lowest with 5 (3.8%). (4) Forty or 2.8% of 1,417 male worms were found to have 2-4 cecal loops. The cecal union located in 71-80% of worm bodys from the anterior end of male worm was found in highest number; the next in 61-70%; and the lowest in 51-60% and 81-90%. The location of united ceca in female worms was found all in the posterior part of body. (5) The ovary in all 125 females was located in the posterior portion of worm body except in 4 specimens. (6) The number of eggs in the uterus of female worms varied from 50 to 300. The mean number was 103.", "PMID": 977230}
{"id": "pubmed23n0032_11034", "title": "An international survey of leptospiral agglutinin of dogs by RMAT.", "content": "FRom 1970 through 1973, the author used RMAT to examine the canine leptospiral antibodies against 10 serotypes in 12,374 attended dogs, 1,855 stray dogs and 480 house dogs in 24 countries. Among the 10 serotypes, L. icterohaemorrhagiae and L. canicola were the most prevalent. Positive rates were in the following order: Peru 26.3%, Chile 18.4%, Turkey 17.4%, Austria 16.0%, Korea 15.0%, Cina (Taiwan) 11.4%, Japan 9.8%, Brazil 7.7%, United States 5.5%, United Kingdom 4.5%, Italy 3.5%, Germany 2.0%, and Canada 0.4%. Negative results were obtained in Australia, Dominica, Ecuador, Ethiopia, Fiji, Indonesia, Iran, Malta, Philippines, Sweden and Thailand. Positive rates on different continents were: South America 11.1%, Asia 10.1%, Europe 3.4%, North America 2.4%, Oceania and Africa (Ethiopia) 0%. Negative results were obtained against either L. icterohaemorrhagiae or L. canicola in samples from most tropical countries. Lower positive rates were seen during summer in Korea and Japan. Positive reactors were detected against other serotypes, viz., L. grippotyphosa, L. australis A, L. pyrogenes, L. bataviae, L. autumnalis, L. hebdomadis and L. javanica. Sex, physical condition and vaccination status as factors in the presence of antibodies were reviewed.", "contents": "An international survey of leptospiral agglutinin of dogs by RMAT. FRom 1970 through 1973, the author used RMAT to examine the canine leptospiral antibodies against 10 serotypes in 12,374 attended dogs, 1,855 stray dogs and 480 house dogs in 24 countries. Among the 10 serotypes, L. icterohaemorrhagiae and L. canicola were the most prevalent. Positive rates were in the following order: Peru 26.3%, Chile 18.4%, Turkey 17.4%, Austria 16.0%, Korea 15.0%, Cina (Taiwan) 11.4%, Japan 9.8%, Brazil 7.7%, United States 5.5%, United Kingdom 4.5%, Italy 3.5%, Germany 2.0%, and Canada 0.4%. Negative results were obtained in Australia, Dominica, Ecuador, Ethiopia, Fiji, Indonesia, Iran, Malta, Philippines, Sweden and Thailand. Positive rates on different continents were: South America 11.1%, Asia 10.1%, Europe 3.4%, North America 2.4%, Oceania and Africa (Ethiopia) 0%. Negative results were obtained against either L. icterohaemorrhagiae or L. canicola in samples from most tropical countries. Lower positive rates were seen during summer in Korea and Japan. Positive reactors were detected against other serotypes, viz., L. grippotyphosa, L. australis A, L. pyrogenes, L. bataviae, L. autumnalis, L. hebdomadis and L. javanica. Sex, physical condition and vaccination status as factors in the presence of antibodies were reviewed.", "PMID": 977231}
{"id": "pubmed23n0032_11035", "title": "Influenza virus isolations from dogs during a human epidemic in Taiwan.", "content": "During an islandwide outbreak of human influenza in June and July 1971, throat swabs were taken from dogs and cats in 3 urban communities of Taiwan. Eight influenza isolates were obtained from dogs in July 1971: two were identified as influenza A closely related to the human Hong Kong/68 virus, and one was a strain of influenza type B closely resembling human influenza B virus. The latter is the first such reported isolation from dogs under natural conditions.", "contents": "Influenza virus isolations from dogs during a human epidemic in Taiwan. During an islandwide outbreak of human influenza in June and July 1971, throat swabs were taken from dogs and cats in 3 urban communities of Taiwan. Eight influenza isolates were obtained from dogs in July 1971: two were identified as influenza A closely related to the human Hong Kong/68 virus, and one was a strain of influenza type B closely resembling human influenza B virus. The latter is the first such reported isolation from dogs under natural conditions.", "PMID": 977232}
{"id": "pubmed23n0032_11036", "title": "Thyroid carcinoma.", "content": "Pathological and clinical characteristics of thyroid carcinoma are reported. In 379 routine consecutive autopsies in which carcinoma was not suspected, serial sections of thyroid gland were histologically examined. Latent thyroid carcinoma was detected in 15.7% of the glands. Papillary adenocarcinoma comprised 76.3%, follicular adenocarcinoma 22.0% and trabecular carcinoma 1.7% of the cases. Sclerosing carcinoma was seen in 32 cases. The long diameter of 90% of these tumors was less than 5 mm. Whether or not thyroid carcinoma persists as such a small carcinomatous lesion indefinitely was studied along with its clinical significance. Carcinomatous lesions complicating hyperthyroidism were always less than 1 cm. About 70% were less than 5 mm long. Since proliferation of some thyroid carcinomas was inhibited by TSH suppression therapy, growth and proliferation of carcinoma in the presence of excessive thyroid hormone or hyperthyroidism is probably already inhibited.", "contents": "Thyroid carcinoma. Pathological and clinical characteristics of thyroid carcinoma are reported. In 379 routine consecutive autopsies in which carcinoma was not suspected, serial sections of thyroid gland were histologically examined. Latent thyroid carcinoma was detected in 15.7% of the glands. Papillary adenocarcinoma comprised 76.3%, follicular adenocarcinoma 22.0% and trabecular carcinoma 1.7% of the cases. Sclerosing carcinoma was seen in 32 cases. The long diameter of 90% of these tumors was less than 5 mm. Whether or not thyroid carcinoma persists as such a small carcinomatous lesion indefinitely was studied along with its clinical significance. Carcinomatous lesions complicating hyperthyroidism were always less than 1 cm. About 70% were less than 5 mm long. Since proliferation of some thyroid carcinomas was inhibited by TSH suppression therapy, growth and proliferation of carcinoma in the presence of excessive thyroid hormone or hyperthyroidism is probably already inhibited.", "PMID": 977240}
{"id": "pubmed23n0032_11037", "title": "Termination of pregnancy in the midtrimester using a new technic. Preliminary report.", "content": "This report is a preliminary one because the number of cases is small (30+) and follow-up is not adequate, either as to detail or time. However, the initial results and observations are so favorable that unless untoward complications arise, we predict that this procedure will supplant the other usual methods of midtrimester abortion. We have looked for but have not found any infections, hemorrhage, perforations, failure of the uterus to empty within a reasonable length of time or prolonged postoperative bleeding. The method herein proposed offers greater safety by creating fewer risks to the patient; her exposure to hazards is therefore diminished. Future pregnancies are safer because the birth canal has not been attenuated by rupture, near rupture, perforation or surgical scarring. It is our hope that others will try this technic to prove or disprove its efficacy.", "contents": "Termination of pregnancy in the midtrimester using a new technic. Preliminary report. This report is a preliminary one because the number of cases is small (30+) and follow-up is not adequate, either as to detail or time. However, the initial results and observations are so favorable that unless untoward complications arise, we predict that this procedure will supplant the other usual methods of midtrimester abortion. We have looked for but have not found any infections, hemorrhage, perforations, failure of the uterus to empty within a reasonable length of time or prolonged postoperative bleeding. The method herein proposed offers greater safety by creating fewer risks to the patient; her exposure to hazards is therefore diminished. Future pregnancies are safer because the birth canal has not been attenuated by rupture, near rupture, perforation or surgical scarring. It is our hope that others will try this technic to prove or disprove its efficacy.", "PMID": 977241}
{"id": "pubmed23n0032_11038", "title": "Microrheology of erythrocytes, blood viscosity, and the distribution of blood flow in the microcirculation.", "content": "The normal rheological behavior of the red blood cells is prerequisite for the survival of the red cells and also for the functioning of microcirculation. Severe alterations of red cell deformability are incompatible with life. When compensated by anemia, even relatively severe rheological incompetence of individual red cells is tolerable. Functional loss of red cell deformability is widely known to occur under conditions of sustained hypoperfusion, and disseminated stagnation of blood in the paracapillary bed occurs. The resulting capillary occlusion does not necessarily reveal itself in grossly reduced flow rates or increased \"total peripheral resistance,\" since it is compensated by shunting through microscopic anastomoses. The biological significance of the phenomenon of red cell aggregation (collateral loss of blood fluidity, \"collateral blood viscidation\") is related to hemodynamics only on the level of individual capillaries. Since the compensatory potentials of vasomotor factors at this level are also very high, the collateral viscidation is not only facilitated but by the same token partially compensated. Therefore, unless complicated by a defect in the macrocirculation, the biological significance of blood rheology seen under the aspects of entire organs is not primarily related to hemodynamics, but to diffusive transcapillary exchange. As a consequence of collateral blood viscidation, diffusion takes place under sub-optimal conditions. The available surface area for exchange is reduced, the diffusion distances are increased. In themselves, these changes are no acute threat to the survival of the entire individual. In combination with other defects, they are capable of sustaining prolonged states of flow arrest. Since the rheological properties of blood can be easily manipulated, sustained circulatory deficiencies can be avoided or treated by improving blood fluidity.", "contents": "Microrheology of erythrocytes, blood viscosity, and the distribution of blood flow in the microcirculation. The normal rheological behavior of the red blood cells is prerequisite for the survival of the red cells and also for the functioning of microcirculation. Severe alterations of red cell deformability are incompatible with life. When compensated by anemia, even relatively severe rheological incompetence of individual red cells is tolerable. Functional loss of red cell deformability is widely known to occur under conditions of sustained hypoperfusion, and disseminated stagnation of blood in the paracapillary bed occurs. The resulting capillary occlusion does not necessarily reveal itself in grossly reduced flow rates or increased \"total peripheral resistance,\" since it is compensated by shunting through microscopic anastomoses. The biological significance of the phenomenon of red cell aggregation (collateral loss of blood fluidity, \"collateral blood viscidation\") is related to hemodynamics only on the level of individual capillaries. Since the compensatory potentials of vasomotor factors at this level are also very high, the collateral viscidation is not only facilitated but by the same token partially compensated. Therefore, unless complicated by a defect in the macrocirculation, the biological significance of blood rheology seen under the aspects of entire organs is not primarily related to hemodynamics, but to diffusive transcapillary exchange. As a consequence of collateral blood viscidation, diffusion takes place under sub-optimal conditions. The available surface area for exchange is reduced, the diffusion distances are increased. In themselves, these changes are no acute threat to the survival of the entire individual. In combination with other defects, they are capable of sustaining prolonged states of flow arrest. Since the rheological properties of blood can be easily manipulated, sustained circulatory deficiencies can be avoided or treated by improving blood fluidity.", "PMID": 977248}
{"id": "pubmed23n0032_11039", "title": "Choline acetyltransferase in ocular tissues of rabbits, cats, cattle, and man.", "content": "The variation of choline acetyltransferase activity in ocular tissues of four mammalian species, rabbits, cats, cattle, and man, was determined. Enzyme activity of irides and ciliary bodies, i.e., parasympathetically innervated structures, tended to be similar in all four species. Two exceptions were bovine irides and human ciliary bodies; these two tissues had higher enzyme activities. Choline acetyltransferase activity was present in the corneal epithelium of rabbit, bovine, and human eyes, but little or none could be detected in that of cats. Feline retina and pigment epithelium-choroid also contained far less choline acetyltransferase activity than the same tissues in the other three species.", "contents": "Choline acetyltransferase in ocular tissues of rabbits, cats, cattle, and man. The variation of choline acetyltransferase activity in ocular tissues of four mammalian species, rabbits, cats, cattle, and man, was determined. Enzyme activity of irides and ciliary bodies, i.e., parasympathetically innervated structures, tended to be similar in all four species. Two exceptions were bovine irides and human ciliary bodies; these two tissues had higher enzyme activities. Choline acetyltransferase activity was present in the corneal epithelium of rabbit, bovine, and human eyes, but little or none could be detected in that of cats. Feline retina and pigment epithelium-choroid also contained far less choline acetyltransferase activity than the same tissues in the other three species.", "PMID": 977252}
{"id": "pubmed23n0032_11040", "title": "Comparison of ocular prostaglandin synthesis inhibitors.", "content": "A comparative study was carried out of the inhibitory effect of 14 nonsteroidal anti-inflammatory compounds, administered topically, on the intraocular pressure and aqueous humor protein elevation induced by arachidonic acid in rabbits. The most effective agents were flurbiprofen solution and suspensions of indoxole (polysorbate-dispersed), meclofenamic acid, indomethacin, and clonixin.", "contents": "Comparison of ocular prostaglandin synthesis inhibitors. A comparative study was carried out of the inhibitory effect of 14 nonsteroidal anti-inflammatory compounds, administered topically, on the intraocular pressure and aqueous humor protein elevation induced by arachidonic acid in rabbits. The most effective agents were flurbiprofen solution and suspensions of indoxole (polysorbate-dispersed), meclofenamic acid, indomethacin, and clonixin.", "PMID": 977253}
{"id": "pubmed23n0032_11041", "title": "A comparison between an acetazolamide test and weight tonography in pathological and apathological circulation of the aqueous humor.", "content": "In a total of 92 eyes in 46 individuals the outflow facilities obtained by weight tonography, Cton correlated curvilinearly with those estimated by an acetazolamide test, Cacet. The presumed apathological pairs of eyes were those with Cacet above (or equal to) 0.15 and pressure symmetry (right/left). Twenty-one patients referred for glaucoma suspicion (and three normal test persons) showed these characteristics. The eyes appeared clinically healthy even if the pressure range reached 30 mm. Hg. Here Cacet averaged 0.32 but Cton only 0.16; the discrepancy is possibly caused by the outflow obstruction brought about by the high pressures during weight tonography. The presumed pathological eyes (33 from 20 individuals referred for glaucoma suspicion or manifest glaucoma) were those with Cacet below 0.15. They generally showed pressure asymmetry and in some cases pressure values above 30 mm. Hg, and there were in several cases other glaucomatous signs. In this group Cton and Cacet were similar; both averaged 0.09. The acetazolamide test is considered more informative than weight tonography because the test provides at the same time an estimate of the outflow facilities in the normal- or low-pressure range as well as an accurate comparison between the pressures and outflow facilities of the two eyes. The test is time-consuming, however (1 to 11/2 hours).", "contents": "A comparison between an acetazolamide test and weight tonography in pathological and apathological circulation of the aqueous humor. In a total of 92 eyes in 46 individuals the outflow facilities obtained by weight tonography, Cton correlated curvilinearly with those estimated by an acetazolamide test, Cacet. The presumed apathological pairs of eyes were those with Cacet above (or equal to) 0.15 and pressure symmetry (right/left). Twenty-one patients referred for glaucoma suspicion (and three normal test persons) showed these characteristics. The eyes appeared clinically healthy even if the pressure range reached 30 mm. Hg. Here Cacet averaged 0.32 but Cton only 0.16; the discrepancy is possibly caused by the outflow obstruction brought about by the high pressures during weight tonography. The presumed pathological eyes (33 from 20 individuals referred for glaucoma suspicion or manifest glaucoma) were those with Cacet below 0.15. They generally showed pressure asymmetry and in some cases pressure values above 30 mm. Hg, and there were in several cases other glaucomatous signs. In this group Cton and Cacet were similar; both averaged 0.09. The acetazolamide test is considered more informative than weight tonography because the test provides at the same time an estimate of the outflow facilities in the normal- or low-pressure range as well as an accurate comparison between the pressures and outflow facilities of the two eyes. The test is time-consuming, however (1 to 11/2 hours).", "PMID": 977254}
{"id": "pubmed23n0032_11042", "title": "Potentiation of the effects of topical epinephrine on the pupil and intraocular pressure in the sympathetically denervated rabbit eye by a catechol-O-methyl transferase inhibitor.", "content": "Dose-response curves of increase in pupil size and decrease in intraocular pressure with topical epinephrine have been determined in the sympathetically denervated rabbit eye. Topical pretreatment with the catechol-O-methyl transferase inhibitor U-0521 potentiated the effects of epinephrine on both the pupil and pressure. These observations suggest a possible role for catechol-O-methyl transferase in the aqueous humor dynamics of the supersensitive eye. The possible use of the denervated rabbit eye as an experimental model for the glaucomatous eye in evaluating the ocular effects of adrenergic agents is discussed.", "contents": "Potentiation of the effects of topical epinephrine on the pupil and intraocular pressure in the sympathetically denervated rabbit eye by a catechol-O-methyl transferase inhibitor. Dose-response curves of increase in pupil size and decrease in intraocular pressure with topical epinephrine have been determined in the sympathetically denervated rabbit eye. Topical pretreatment with the catechol-O-methyl transferase inhibitor U-0521 potentiated the effects of epinephrine on both the pupil and pressure. These observations suggest a possible role for catechol-O-methyl transferase in the aqueous humor dynamics of the supersensitive eye. The possible use of the denervated rabbit eye as an experimental model for the glaucomatous eye in evaluating the ocular effects of adrenergic agents is discussed.", "PMID": 977255}
{"id": "pubmed23n0032_11043", "title": "Corneal sensitivity in people with the same and with different iris color.", "content": "Corneal touch thresholds were determined for both eyes of 15 people with different colored irides (a condition known as heterochromia) and of 25 people with similar colored irides. It was found that corneal sensitivity is not significantly different in both eyes of either group. Therefore it is suggested that the fact previously demonstrated that corneal sensitivity is related to iris color is not of corneal origin.", "contents": "Corneal sensitivity in people with the same and with different iris color. Corneal touch thresholds were determined for both eyes of 15 people with different colored irides (a condition known as heterochromia) and of 25 people with similar colored irides. It was found that corneal sensitivity is not significantly different in both eyes of either group. Therefore it is suggested that the fact previously demonstrated that corneal sensitivity is related to iris color is not of corneal origin.", "PMID": 977256}
{"id": "pubmed23n0032_11044", "title": "The effect of tonicity on lens volume.", "content": "Rat lenses incubated in hypotonic medium adapted to the medium by first undergoing osmotic swelling and then returning after 1 to 2 days to their originial volume. Two processes--osmotic swelling and volume regulation--appear to be working simultaneously. The former is indicated by a decrease in the concentration of cell potassium to the expected level and the latter by a limitation of lens volume associated with a decrease in the potassium content of the lens. These findings are consistent with those observed in other types of cells and may be of significance in understanding the etiology of cataracts.", "contents": "The effect of tonicity on lens volume. Rat lenses incubated in hypotonic medium adapted to the medium by first undergoing osmotic swelling and then returning after 1 to 2 days to their originial volume. Two processes--osmotic swelling and volume regulation--appear to be working simultaneously. The former is indicated by a decrease in the concentration of cell potassium to the expected level and the latter by a limitation of lens volume associated with a decrease in the potassium content of the lens. These findings are consistent with those observed in other types of cells and may be of significance in understanding the etiology of cataracts.", "PMID": 977257}
{"id": "pubmed23n0032_11045", "title": "Hormonal influences on photoreceptor damage: the pituitary gland and ovaries.", "content": "To determine whether the absence of pituitary or ovarian hormones would influence retinal degeneration, female albino rats were either hypophysectomized (HYPEX) or ovariectomized (OVEX) before pubery. Later, they were exposed to continuous light for periods up to 45 days. Retinas evaluated by light microscopic measurements showed damage to the outer nuclear layer (ONL) and photoreceptor layer in both the operated and intact, control rats. However, the degree of damage observed in retinas of HYPEX and OVEX rats was significantly less than that observed in retinas of intact rats exposed to the same lighting conditions. Therefore, hypophysectomy and ovariectomy, which influence the normal development of sexual maturation when performed on immature rats, significantly reduce photoreceptor damage in adult rats exposed to continuous light.", "contents": "Hormonal influences on photoreceptor damage: the pituitary gland and ovaries. To determine whether the absence of pituitary or ovarian hormones would influence retinal degeneration, female albino rats were either hypophysectomized (HYPEX) or ovariectomized (OVEX) before pubery. Later, they were exposed to continuous light for periods up to 45 days. Retinas evaluated by light microscopic measurements showed damage to the outer nuclear layer (ONL) and photoreceptor layer in both the operated and intact, control rats. However, the degree of damage observed in retinas of HYPEX and OVEX rats was significantly less than that observed in retinas of intact rats exposed to the same lighting conditions. Therefore, hypophysectomy and ovariectomy, which influence the normal development of sexual maturation when performed on immature rats, significantly reduce photoreceptor damage in adult rats exposed to continuous light.", "PMID": 977258}
{"id": "pubmed23n0032_11046", "title": "Photosensitive pigments formed with rat opsin.", "content": "Photosensitive pigments are formed in vitro from rat opsin with the rods in suspension or as digitonin solutions. Rhodopsin, isorhodopsin I, and isorhodopsin II were generated with 11-cis, 9-cis and 9, 13-dicis retinal, respectively. An isorhodopsin I analogue was formed on combination with 9-cis 13-desmethyl-14-methylretinal. The absorption spectra and photosensitivities of these pigments were found to be similar to the corresponding bovine pigments although the rates of formation are several fold slower.", "contents": "Photosensitive pigments formed with rat opsin. Photosensitive pigments are formed in vitro from rat opsin with the rods in suspension or as digitonin solutions. Rhodopsin, isorhodopsin I, and isorhodopsin II were generated with 11-cis, 9-cis and 9, 13-dicis retinal, respectively. An isorhodopsin I analogue was formed on combination with 9-cis 13-desmethyl-14-methylretinal. The absorption spectra and photosensitivities of these pigments were found to be similar to the corresponding bovine pigments although the rates of formation are several fold slower.", "PMID": 977259}
{"id": "pubmed23n0032_11047", "title": "The allergic theory of radiocontrast agent toxicity: demonstration of antibody activity in sera of patients suffering major radiocontrast agent reactions.", "content": "An allergic, immunologic mechanism for certain adverse reactions to radiocontrast agents has been postulated on the basis of clinical and theoretical considerations. One obstacle to the acceptance of this allergic theory has been the inability to demonstrate significant antibodies with specificity for these agents. In our studies, rabbits were immunized with 4 analogues of radiocontrast agents which induced IgG or IgE antibodies specific for the contrast media analogues. Then, to determine if antibodies to contrast agents were present in man a prospective, one-year surveillance of radiocontrast agent reactions was undertaken. Sera from 27 patients suffering severe reactions and 37 control subjects were analyzed by radioimmunoassay for antibodies reactive with radiocontrast media. Binding of radiolabeled contrast media was significantly elevated (p less than 0.01) by the serum globulin fraction of the patients suffering severe reactions. Demonstration of this antibody activity, although not proof of cause and effect between the presence of antibody activity and clinical symptoms, nonetheless adds support to the hypothesis that some adverse reactions to radiocontrast agents are due to antibodies.", "contents": "The allergic theory of radiocontrast agent toxicity: demonstration of antibody activity in sera of patients suffering major radiocontrast agent reactions. An allergic, immunologic mechanism for certain adverse reactions to radiocontrast agents has been postulated on the basis of clinical and theoretical considerations. One obstacle to the acceptance of this allergic theory has been the inability to demonstrate significant antibodies with specificity for these agents. In our studies, rabbits were immunized with 4 analogues of radiocontrast agents which induced IgG or IgE antibodies specific for the contrast media analogues. Then, to determine if antibodies to contrast agents were present in man a prospective, one-year surveillance of radiocontrast agent reactions was undertaken. Sera from 27 patients suffering severe reactions and 37 control subjects were analyzed by radioimmunoassay for antibodies reactive with radiocontrast media. Binding of radiolabeled contrast media was significantly elevated (p less than 0.01) by the serum globulin fraction of the patients suffering severe reactions. Demonstration of this antibody activity, although not proof of cause and effect between the presence of antibody activity and clinical symptoms, nonetheless adds support to the hypothesis that some adverse reactions to radiocontrast agents are due to antibodies.", "PMID": 977260}
{"id": "pubmed23n0032_11048", "title": "The capability of fluoroscopic systems for the production of computerized axial tomograms.", "content": "Previous work in our laboratories and at other institutions has shown that fluoroscopic images recorded on a video disc can be used successfully for producing computerized-axial-tomograms. The work described in this paper gives a quantitative analysis of the capabilities of such imaging systems, in conjunction with a particular method of data processing, for detecting and imaging changes in object absorptivity. Relations between the degree of contrast or absorptivity and object size required by this type of system can be inferred from the data.", "contents": "The capability of fluoroscopic systems for the production of computerized axial tomograms. Previous work in our laboratories and at other institutions has shown that fluoroscopic images recorded on a video disc can be used successfully for producing computerized-axial-tomograms. The work described in this paper gives a quantitative analysis of the capabilities of such imaging systems, in conjunction with a particular method of data processing, for detecting and imaging changes in object absorptivity. Relations between the degree of contrast or absorptivity and object size required by this type of system can be inferred from the data.", "PMID": 977261}
{"id": "pubmed23n0032_11049", "title": "The role of cholecystokinin in radiographic opacification of the gallbladder.", "content": "Studies of the effect of cholecystokinin (CCK) on hepatic elimination of intravenously administered iopanoate and on gallbladder opacification were performed using nonoperated dogs with intact enterohepatic circulation and normal endogenous bile salt pool. Intravenous administration of CCK (3 units/kg) resulted in a 32% increase in apparent transport maximum (maximum rate of elimination) of iopanoate and earlier and enhanced gallbladder opacification. This increase in apparent transport maximum was abolished by cholecystectomy, indicating that the increase was a result of the release of bile salts from the gallbladder rather than a direct effect of the hormone on the hepatic elimination of iopanoate. The early gallbladder opacification and increased density of contrast material in the gallbladder were related to CCK-induced emptying of the gallbladder and subsequent filling with opacified bile.", "contents": "The role of cholecystokinin in radiographic opacification of the gallbladder. Studies of the effect of cholecystokinin (CCK) on hepatic elimination of intravenously administered iopanoate and on gallbladder opacification were performed using nonoperated dogs with intact enterohepatic circulation and normal endogenous bile salt pool. Intravenous administration of CCK (3 units/kg) resulted in a 32% increase in apparent transport maximum (maximum rate of elimination) of iopanoate and earlier and enhanced gallbladder opacification. This increase in apparent transport maximum was abolished by cholecystectomy, indicating that the increase was a result of the release of bile salts from the gallbladder rather than a direct effect of the hormone on the hepatic elimination of iopanoate. The early gallbladder opacification and increased density of contrast material in the gallbladder were related to CCK-induced emptying of the gallbladder and subsequent filling with opacified bile.", "PMID": 977262}
{"id": "pubmed23n0032_11050", "title": "Hearing loss due to combined effects of noise and sodium salicylate.", "content": "This study reports on hearing loss due to the combined effects of noise and sodium salicylate. A group of 10 adult chinchillas was used to test the hypothesis that the combined effects of prolonged exposure to noise and continuous salicylate intoxication will result in a larger Temporary Threshold Shift (TTS) in the audibility curve than either of the agents alone can produce. Extensive behavioral training was used to determine hearing thresholds. Though use of shock avoidance techniques, the animals were trained to respond to tones by jumping a low barrier. After demonstrating sufficient ability in behavioral training, the animals were surgically rendered monaural. After recovery from surgery, monaural threshold data were obtained. The animals were exposed in a chamber to 85 dbA broadband noise for 48 h to measure TTS. The chinchillas were allowed to recover for a minimum of 45 days; then a second monaural audibility curve was obtained. The chinchillas were then injected subcutaneously with sodium salicylate at 6 h intervals to establish TTS-producing serum salicylate levels. After 36 h of salicylate exposure, the animals were placed in a noise chamber. For the next 42 h the animals were exposed to both sodium salicylate and 85 dbA noise. The results of this study indicated the chinchillas' hearing threshold is reduced in sensitivity by approximately 35 dB due to broad-band noise exposure of 85 dBA intensity. Serum salicylate levels of 20-40 mg per 100 ml resulted in a TTS of 30 dB on the average. The combination of noise and sodium salicylate exposure produced a temporary hearing loss of approximately 55 dB. These data suggested that the combination of prolonged noise and salicylate exposure may result in a larger hearing loss than either agent alone can produce.", "contents": "Hearing loss due to combined effects of noise and sodium salicylate. This study reports on hearing loss due to the combined effects of noise and sodium salicylate. A group of 10 adult chinchillas was used to test the hypothesis that the combined effects of prolonged exposure to noise and continuous salicylate intoxication will result in a larger Temporary Threshold Shift (TTS) in the audibility curve than either of the agents alone can produce. Extensive behavioral training was used to determine hearing thresholds. Though use of shock avoidance techniques, the animals were trained to respond to tones by jumping a low barrier. After demonstrating sufficient ability in behavioral training, the animals were surgically rendered monaural. After recovery from surgery, monaural threshold data were obtained. The animals were exposed in a chamber to 85 dbA broadband noise for 48 h to measure TTS. The chinchillas were allowed to recover for a minimum of 45 days; then a second monaural audibility curve was obtained. The chinchillas were then injected subcutaneously with sodium salicylate at 6 h intervals to establish TTS-producing serum salicylate levels. After 36 h of salicylate exposure, the animals were placed in a noise chamber. For the next 42 h the animals were exposed to both sodium salicylate and 85 dbA noise. The results of this study indicated the chinchillas' hearing threshold is reduced in sensitivity by approximately 35 dB due to broad-band noise exposure of 85 dBA intensity. Serum salicylate levels of 20-40 mg per 100 ml resulted in a TTS of 30 dB on the average. The combination of noise and sodium salicylate exposure produced a temporary hearing loss of approximately 55 dB. These data suggested that the combination of prolonged noise and salicylate exposure may result in a larger hearing loss than either agent alone can produce.", "PMID": 977270}
{"id": "pubmed23n0032_11051", "title": "An investigation of the servobehavior of the stretch reflex.", "content": "The stretch reflex is often described as a device for assisting in the regulation of muscle length or as a spinal servomechanism. Observation of the response to experimental interruption of voluntary movements fails to demonstrate a significant role for spinal reflexes. Functional responses with a latency of 120 ms, implying supraspinal mediation, are observed.", "contents": "An investigation of the servobehavior of the stretch reflex. The stretch reflex is often described as a device for assisting in the regulation of muscle length or as a spinal servomechanism. Observation of the response to experimental interruption of voluntary movements fails to demonstrate a significant role for spinal reflexes. Functional responses with a latency of 120 ms, implying supraspinal mediation, are observed.", "PMID": 977271}
{"id": "pubmed23n0032_11052", "title": "Biological disposition of perfluoroctylbromide: tracheal administration in alveolography and bronchography.", "content": "Perfluoroctylbromide, given tracheally either as neat liquid in alveolography or as 10:1 emulsion in bronchography, was cleared roentgenologically from the lungs within 24 hours. Quantitative analysis was performed on a gas chromatograph. The rats were given either 2 ml/kg of neat liquid or 0.4 ml/kg emulsion. One per cent of the neat liquid and 8% of the emulsion were recovered from the tissues respectively 24 hours later. The lungs had the largest amount followed by the intestine, adipose tissue and lymph nodes. Dogs were sacrificed at various intervals following 4 ml/kg of neat liquid or 2 ml/kg of emulsion. Less than 1% of the dose was found in the tissues at 24 hours. A similar pattern of perfluoroctylbromide distribution was observed as in the rats. The tissue contents of perfluoroctylbromide at three months were below the limit of detection. The perfluoroctylbromide levels in the blood and urine of dogs and human volunteers were either non-detectable or barely detectable over a 48-hour period.", "contents": "Biological disposition of perfluoroctylbromide: tracheal administration in alveolography and bronchography. Perfluoroctylbromide, given tracheally either as neat liquid in alveolography or as 10:1 emulsion in bronchography, was cleared roentgenologically from the lungs within 24 hours. Quantitative analysis was performed on a gas chromatograph. The rats were given either 2 ml/kg of neat liquid or 0.4 ml/kg emulsion. One per cent of the neat liquid and 8% of the emulsion were recovered from the tissues respectively 24 hours later. The lungs had the largest amount followed by the intestine, adipose tissue and lymph nodes. Dogs were sacrificed at various intervals following 4 ml/kg of neat liquid or 2 ml/kg of emulsion. Less than 1% of the dose was found in the tissues at 24 hours. A similar pattern of perfluoroctylbromide distribution was observed as in the rats. The tissue contents of perfluoroctylbromide at three months were below the limit of detection. The perfluoroctylbromide levels in the blood and urine of dogs and human volunteers were either non-detectable or barely detectable over a 48-hour period.", "PMID": 977265}
{"id": "pubmed23n0032_11053", "title": "The mechanism of hypotension following angiography.", "content": "The hypotension observed following the intracardiac injection of contrast media is believed to be the result of myocardial depression, peripheral vasodilatation, or both. It is unclear which of these factors is primarily involved in different types of angiography. To assess this problem, Renografin-76 was injected into the aortic root of 15 anesthetized dogs (1 ml/kg) and into the left coronary artery (3-5 ml) in 18 dogs. The heart rate, the systolic and diastolic arterial pressures, left ventricular pressure, peak dp/dt and dp/dt-40 were monitored continuously until return to baseline. Following selective left coronary artery injection, both systolic and diastolic pressures decreased maximally at 5 seconds with parallel changes in peak dp/dt and dp/dt-40. Following the aortic root injection, the blood pressure response was biphasic, increasing at 5 seconds and decreasing maximally at 20 seconds. The corresponding changes in peak dp/dt and dp/dt-40 were initial depression at 5 seconds with quick return to baseline and increase above control at the time of maximal hypotension. The left ventricular end diastolic pressure rose maximally at 5 to 10 seconds for both coronary and aortic injections. The mechanism of hypotension, therefore, varies with the type of angiography. Myocardial depression appears to be a primary factor with selective coronary angiography. However, with aortic root injection, myocardial contractility, although initially depressed, is already improved at the time of maximal hypotension, and thus peripheral vasocilatation is probably the prime mechanism of the hypotension.", "contents": "The mechanism of hypotension following angiography. The hypotension observed following the intracardiac injection of contrast media is believed to be the result of myocardial depression, peripheral vasodilatation, or both. It is unclear which of these factors is primarily involved in different types of angiography. To assess this problem, Renografin-76 was injected into the aortic root of 15 anesthetized dogs (1 ml/kg) and into the left coronary artery (3-5 ml) in 18 dogs. The heart rate, the systolic and diastolic arterial pressures, left ventricular pressure, peak dp/dt and dp/dt-40 were monitored continuously until return to baseline. Following selective left coronary artery injection, both systolic and diastolic pressures decreased maximally at 5 seconds with parallel changes in peak dp/dt and dp/dt-40. Following the aortic root injection, the blood pressure response was biphasic, increasing at 5 seconds and decreasing maximally at 20 seconds. The corresponding changes in peak dp/dt and dp/dt-40 were initial depression at 5 seconds with quick return to baseline and increase above control at the time of maximal hypotension. The left ventricular end diastolic pressure rose maximally at 5 to 10 seconds for both coronary and aortic injections. The mechanism of hypotension, therefore, varies with the type of angiography. Myocardial depression appears to be a primary factor with selective coronary angiography. However, with aortic root injection, myocardial contractility, although initially depressed, is already improved at the time of maximal hypotension, and thus peripheral vasocilatation is probably the prime mechanism of the hypotension.", "PMID": 977264}
{"id": "pubmed23n0032_11054", "title": "The control and propagation of a genetic disease.", "content": "Preventive health is an appealing method to better our quality of life. However, in the practice of preventive health, its probabilistic nature and the time lag for results lessen its appeal. The specter of genetic diseases also has a probabilistic nature but has a time lag that spans generations. Thus any preventive health effort for genetic diseases should be studied so the results can be predicted in advance. A number of mathematical genetic problems have been solved that include mutation and phenotype selection factors. This paper takes a large human population that has birth and death frequencies that vary with age and that operates under Mendelian genetics. Hypothetical mutation and backmutation rates are put into this population; this results in an adverse effect on the death rate for those individuals born with homogenously mutated genes. This is called a genetic defect. The mutated genes are carried in the gametes of individuals who are born with the genetic defect and those who are exposed to the mutation rates; their manifestation is an increased probability of producing method gametes for reproduction. The preventive health control effort is birth control after genetic counseling. Various combinations of birth control selection effectiveness are tried on the different genotypes. It is found that a high selection factor for homogenously mutated genotypes gives the best result for reducing excess deaths caused by the genetic defect, and that a selection factor for heterozygous genotypes is a counterproductive effort. This model, employing a set of difference equations, may be used to evaluate preventive health policies on the control of genetically linked diseases such as sickle cell anemia, which affect the death rate of afflicted individuals. Also, effects other than death rate may be incorporated to evaluate policies on genetic diseases such as retinal blastoma.", "contents": "The control and propagation of a genetic disease. Preventive health is an appealing method to better our quality of life. However, in the practice of preventive health, its probabilistic nature and the time lag for results lessen its appeal. The specter of genetic diseases also has a probabilistic nature but has a time lag that spans generations. Thus any preventive health effort for genetic diseases should be studied so the results can be predicted in advance. A number of mathematical genetic problems have been solved that include mutation and phenotype selection factors. This paper takes a large human population that has birth and death frequencies that vary with age and that operates under Mendelian genetics. Hypothetical mutation and backmutation rates are put into this population; this results in an adverse effect on the death rate for those individuals born with homogenously mutated genes. This is called a genetic defect. The mutated genes are carried in the gametes of individuals who are born with the genetic defect and those who are exposed to the mutation rates; their manifestation is an increased probability of producing method gametes for reproduction. The preventive health control effort is birth control after genetic counseling. Various combinations of birth control selection effectiveness are tried on the different genotypes. It is found that a high selection factor for homogenously mutated genotypes gives the best result for reducing excess deaths caused by the genetic defect, and that a selection factor for heterozygous genotypes is a counterproductive effort. This model, employing a set of difference equations, may be used to evaluate preventive health policies on the control of genetically linked diseases such as sickle cell anemia, which affect the death rate of afflicted individuals. Also, effects other than death rate may be incorporated to evaluate policies on genetic diseases such as retinal blastoma.", "PMID": 977272}
{"id": "pubmed23n0032_11055", "title": "Comparison between mercury and lithium chemical systems for pacemaker energy source applications.", "content": "Over the past decade several types of chemical systems have been developed as energy sources for implantable pacemakers. The most widely used system is the mercuric oxide-zinc cell. Historically, mercury-zinc systems were the first to be used to power an implantable pacemaker; they have undergone several developments and modification in order to improve their performance characteristics. Recently, several new power sources have been designed and developed exclusively for implantable pacemakers, the most promising of which are chemical systems based upon lithium metal as anode material. This paper reviews the basic chemical, physical, and electrical characteristics of mercuric oxide-zinc and lithium based systems and evaluates their suitability for pacemaker applications based upon analysis of their inherent characteristics.", "contents": "Comparison between mercury and lithium chemical systems for pacemaker energy source applications. Over the past decade several types of chemical systems have been developed as energy sources for implantable pacemakers. The most widely used system is the mercuric oxide-zinc cell. Historically, mercury-zinc systems were the first to be used to power an implantable pacemaker; they have undergone several developments and modification in order to improve their performance characteristics. Recently, several new power sources have been designed and developed exclusively for implantable pacemakers, the most promising of which are chemical systems based upon lithium metal as anode material. This paper reviews the basic chemical, physical, and electrical characteristics of mercuric oxide-zinc and lithium based systems and evaluates their suitability for pacemaker applications based upon analysis of their inherent characteristics.", "PMID": 977273}
{"id": "pubmed23n0032_11056", "title": "Ultrasonic visualization of the non-opacified gallbladder: response of the normal and obstructed canine gallbladder to C8CCK.", "content": "In vitro studies of a simulated gallbladder show that B-scan ultrasonography is as reliable as radiography in measuring changes in volume of the organ. The width or planimetric area of the sonogram correlates with the change in balloon volume and with similar radiographic measurements. In vivo measurements of the normal canine gallbladder stimulated by a carefully prescribed infusion of C8CCK in the dose range of 2.2 to 5.5 ng/kg/min over an 8 to 10 minute interval, showed a clearly defined decrease in gallbladder width. In ligation and division of the common bile duct no change in gallbladder size was demonstrated. We conclude that sonography alone can be used to measure a change in gallbladder size to better differentiate complete obstruction of the common bile duct from medical causes in the jaundiced patient.", "contents": "Ultrasonic visualization of the non-opacified gallbladder: response of the normal and obstructed canine gallbladder to C8CCK. In vitro studies of a simulated gallbladder show that B-scan ultrasonography is as reliable as radiography in measuring changes in volume of the organ. The width or planimetric area of the sonogram correlates with the change in balloon volume and with similar radiographic measurements. In vivo measurements of the normal canine gallbladder stimulated by a carefully prescribed infusion of C8CCK in the dose range of 2.2 to 5.5 ng/kg/min over an 8 to 10 minute interval, showed a clearly defined decrease in gallbladder width. In ligation and division of the common bile duct no change in gallbladder size was demonstrated. We conclude that sonography alone can be used to measure a change in gallbladder size to better differentiate complete obstruction of the common bile duct from medical causes in the jaundiced patient.", "PMID": 977263}
{"id": "pubmed23n0032_11057", "title": "Automated rodent respiratory monitor and histogram computer--a preliminary report.", "content": "The system described in this paper was designed to monitor total inhaled volume (V), tidal volume (VT), and respiratory frequency (f) of Fisher rats before, during, and after exposure to cigarette smoke. The systems consists of three major subsystems: plethysmograph, analog signal conditioner, and histogram computer. The volume type whole body plethysmograph incorporates a rubber nose seal. Tidal flow rate (V) from the tube, measured by a pneumotachometer, is integrated by the signal conditioning system to obtain VT and V. Both V and V are displayed on a strip chart recorder. A rat will often exhibit considerable sniffing, especially in the presence of cigarette smoke. Consequently, average tidal volume (VT) and average respiratory frequency (f) are not accurate measurements of typical VT and f. Sensitivity of the system to changes in typical VT and f is important snce the system will be used to evaluate the effects of subtle differences in types of experimental cigarettes. A microprocessor was used in conjunction with a cathode ray tube to compute and display both the VT and respiratory period (T = 1/f) as histograms. From the histograms the most common (typical) VT (mode of the histogram) and the most common T can be easily observed and recorded. The histogram computer also calculates and digitally displays VT and f.", "contents": "Automated rodent respiratory monitor and histogram computer--a preliminary report. The system described in this paper was designed to monitor total inhaled volume (V), tidal volume (VT), and respiratory frequency (f) of Fisher rats before, during, and after exposure to cigarette smoke. The systems consists of three major subsystems: plethysmograph, analog signal conditioner, and histogram computer. The volume type whole body plethysmograph incorporates a rubber nose seal. Tidal flow rate (V) from the tube, measured by a pneumotachometer, is integrated by the signal conditioning system to obtain VT and V. Both V and V are displayed on a strip chart recorder. A rat will often exhibit considerable sniffing, especially in the presence of cigarette smoke. Consequently, average tidal volume (VT) and average respiratory frequency (f) are not accurate measurements of typical VT and f. Sensitivity of the system to changes in typical VT and f is important snce the system will be used to evaluate the effects of subtle differences in types of experimental cigarettes. A microprocessor was used in conjunction with a cathode ray tube to compute and display both the VT and respiratory period (T = 1/f) as histograms. From the histograms the most common (typical) VT (mode of the histogram) and the most common T can be easily observed and recorded. The histogram computer also calculates and digitally displays VT and f.", "PMID": 977275}
{"id": "pubmed23n0032_11058", "title": "A charge coupled transversal filter for biomedical signal conditioning.", "content": "The analysis and processing of biological signals is often hampered by the contamination of these low frequency, often low amplitude waveforms by motion artifact in fluid-filled catheter systems and skin electrodes, power line hum, and so on. Although noise suppression techniques have relied upon low pass filtration of the analog signals with active or passive RLC filters, even the most recent active RLC filter implementations display operational characteristics not entirely satisfactory for low frequency applications.", "contents": "A charge coupled transversal filter for biomedical signal conditioning. The analysis and processing of biological signals is often hampered by the contamination of these low frequency, often low amplitude waveforms by motion artifact in fluid-filled catheter systems and skin electrodes, power line hum, and so on. Although noise suppression techniques have relied upon low pass filtration of the analog signals with active or passive RLC filters, even the most recent active RLC filter implementations display operational characteristics not entirely satisfactory for low frequency applications.", "PMID": 977276}
{"id": "pubmed23n0032_11059", "title": "Comparison of non-screen techniques (medical vs. industrial film) for fine-detail skeletal radiography.", "content": "High resolution radiographic techniques for imaging the peripheral skeleton (hand and foot) have gained wide clinical acceptance. The two procedures receiving widest attention are non-screen techniques: one uses medical film (Kodak RP); and the other uses industrial film (Kodak Type M) combined with optical magnification. The imaging properties and clinical applications of these two techniques were examined. The modulation transfer functions (MTF's) of the recording systems, Wiener spectrum analyses of noise, and film sensitometry were obtained. Clinical comparisons were made from 200 consecutive patients radiographed with both techniques and the relative merits in metabolic, arthritic and traumatic afflictions were assessed. The results demonstrate the superiority of the industrial film compared to medical film technique in all parameters of image quality. However, the inconveniences of special processing and viewing necessitated by this technique, as well as the increased radiation exposure, limit its clinical application to small, selection groups of patients as determined from the clinical comparative study.", "contents": "Comparison of non-screen techniques (medical vs. industrial film) for fine-detail skeletal radiography. High resolution radiographic techniques for imaging the peripheral skeleton (hand and foot) have gained wide clinical acceptance. The two procedures receiving widest attention are non-screen techniques: one uses medical film (Kodak RP); and the other uses industrial film (Kodak Type M) combined with optical magnification. The imaging properties and clinical applications of these two techniques were examined. The modulation transfer functions (MTF's) of the recording systems, Wiener spectrum analyses of noise, and film sensitometry were obtained. Clinical comparisons were made from 200 consecutive patients radiographed with both techniques and the relative merits in metabolic, arthritic and traumatic afflictions were assessed. The results demonstrate the superiority of the industrial film compared to medical film technique in all parameters of image quality. However, the inconveniences of special processing and viewing necessitated by this technique, as well as the increased radiation exposure, limit its clinical application to small, selection groups of patients as determined from the clinical comparative study.", "PMID": 977266}
{"id": "pubmed23n0032_11060", "title": "Physiological data collection system for field use in trained military dogs.", "content": "This research program resulted in synthesis of a complete system for \"in the field\" collection of dynamic physiologic data from trained military dogs. Sensors and sensor implantation techniques were developed for arterial blood pressure, electroencephalogram, electrooculogram, temperature, and respiratory rate monitoring in dogs under strenuous field conditions. A data acquisition system was developed that provided the capability for recording this physiologic data for later computer analysis, and computer programs were written for evaluation of the data. In the final evaluation of the system, two factors indicated a need for additional refinement: (1) that of reducing EEG signal artifact due to cable capacitance and, (2) signal jitter caused by the casette recorder.", "contents": "Physiological data collection system for field use in trained military dogs. This research program resulted in synthesis of a complete system for \"in the field\" collection of dynamic physiologic data from trained military dogs. Sensors and sensor implantation techniques were developed for arterial blood pressure, electroencephalogram, electrooculogram, temperature, and respiratory rate monitoring in dogs under strenuous field conditions. A data acquisition system was developed that provided the capability for recording this physiologic data for later computer analysis, and computer programs were written for evaluation of the data. In the final evaluation of the system, two factors indicated a need for additional refinement: (1) that of reducing EEG signal artifact due to cable capacitance and, (2) signal jitter caused by the casette recorder.", "PMID": 977277}
{"id": "pubmed23n0032_11061", "title": "A rocking motion sensor for the blind.", "content": "Rocking motion is an unconscious and often unwanted phenomenon exhibited by the blind. It is usually manifested in oscillatory back and forth, side to side, or rotatory movements of the upper torso or head at about 1 Hz rate. Subjects desiring to function unobtrusively in the world of the sighted need to be alerted when rocking so they can control it. The rocking motion sensor incorporates biofeedback and records rock occurrences. A miniature body mounted accelerometer senses body accelerations exceeding 0.1g. These motions during a given period, compared with a presettable \"rock threshold\", discriminate rocking from normal activities. Sound biofeedback alerts the subject that he has exceeded threshold. Another counting and timing subsystem determines \"end of rock\" and resets the system. A recorder is provided that records signals only during rocking. Timing information is provided on a separate channel to permit later time line analysis. The rocking motion sensor system utilizes primarily solid state CMOS circuits with a combination of synchronous-asynchronous timing. The recorder is a modified commercial unit. The two units are separate, portable, and belt mounted. Tests have been made and results will be presented.", "contents": "A rocking motion sensor for the blind. Rocking motion is an unconscious and often unwanted phenomenon exhibited by the blind. It is usually manifested in oscillatory back and forth, side to side, or rotatory movements of the upper torso or head at about 1 Hz rate. Subjects desiring to function unobtrusively in the world of the sighted need to be alerted when rocking so they can control it. The rocking motion sensor incorporates biofeedback and records rock occurrences. A miniature body mounted accelerometer senses body accelerations exceeding 0.1g. These motions during a given period, compared with a presettable \"rock threshold\", discriminate rocking from normal activities. Sound biofeedback alerts the subject that he has exceeded threshold. Another counting and timing subsystem determines \"end of rock\" and resets the system. A recorder is provided that records signals only during rocking. Timing information is provided on a separate channel to permit later time line analysis. The rocking motion sensor system utilizes primarily solid state CMOS circuits with a combination of synchronous-asynchronous timing. The recorder is a modified commercial unit. The two units are separate, portable, and belt mounted. Tests have been made and results will be presented.", "PMID": 977278}
{"id": "pubmed23n0032_11062", "title": "Ecophysiological hierarchies.", "content": "Among bovids, antelopes, rodents and marsupials--in fact, probably in most animals--the rates of energy and water turnover are linked to salt tolerance, renal concentration and protein synthesis rates. Evolution in wet areas is associated with high turnover rates and low salt tolerance, while desert derivation goes with low rate functions and high salt tolerance. This basic ecophysiology changes slowly, and animals that migrate to different environments may retain ancient patterns in areas where they seem inappropriate--so that cattle keep their high rates of energy and water use in arid zones, or llamas remain low in energy and water turnover after three million years in cool or wet environments. The rate functions may be linked through gene-controlled rates of protein synthesis and turnover, which evolved to run at high rates in well-supplied areas, and at low rates in the face of the sparse resources of the desert.", "contents": "Ecophysiological hierarchies. Among bovids, antelopes, rodents and marsupials--in fact, probably in most animals--the rates of energy and water turnover are linked to salt tolerance, renal concentration and protein synthesis rates. Evolution in wet areas is associated with high turnover rates and low salt tolerance, while desert derivation goes with low rate functions and high salt tolerance. This basic ecophysiology changes slowly, and animals that migrate to different environments may retain ancient patterns in areas where they seem inappropriate--so that cattle keep their high rates of energy and water use in arid zones, or llamas remain low in energy and water turnover after three million years in cool or wet environments. The rate functions may be linked through gene-controlled rates of protein synthesis and turnover, which evolved to run at high rates in well-supplied areas, and at low rates in the face of the sparse resources of the desert.", "PMID": 977280}
{"id": "pubmed23n0032_11063", "title": "Water conservation in xerophilic birds.", "content": "Adaptation of birds to dry climates involves decrease in relative evaporative water loss (mechanism unknown); decrease in GFR; and high urine osmolality in the dehydrated state in conjunction with cloacal resorption parameters, which allows the urine to enter the cloaca without a further water loss. The net result of the adaptations is such that the best adapted birds, such as the budgerygah (12) and the zebra finch (13) fed dry seeds alone can live without water. Similar features have been observed also in the best adapted desert rodents (27). Finally, birds tolerate a 7 to 13% increase in plasma osmolality (9). Tucker (28) has calculated that the budgerygah has fuel for 14 h flight; we have calculated that it also has water for 14 h (12) at neutral temperature and humidity. This is better than the Jumbo Jet, which can only fly for 12 h.", "contents": "Water conservation in xerophilic birds. Adaptation of birds to dry climates involves decrease in relative evaporative water loss (mechanism unknown); decrease in GFR; and high urine osmolality in the dehydrated state in conjunction with cloacal resorption parameters, which allows the urine to enter the cloaca without a further water loss. The net result of the adaptations is such that the best adapted birds, such as the budgerygah (12) and the zebra finch (13) fed dry seeds alone can live without water. Similar features have been observed also in the best adapted desert rodents (27). Finally, birds tolerate a 7 to 13% increase in plasma osmolality (9). Tucker (28) has calculated that the budgerygah has fuel for 14 h flight; we have calculated that it also has water for 14 h (12) at neutral temperature and humidity. This is better than the Jumbo Jet, which can only fly for 12 h.", "PMID": 977281}
{"id": "pubmed23n0032_11064", "title": "Prediction of equivalent environments by energy exchange and assessments of physiological strain and discomfort.", "content": "The described equivalence postulate was confirmed experimentally by examining physiological responses as well as discomfort sensation in a series of predicted equivalent conditions during exercise (congruent to 3 met). ET or the equivalent Ta at 50% RH is a single independent variable that is uniquely related to the mean skin temperature (Tsk), skin wettedness (w), body core temperature (Tcore) and sense of discomfort.", "contents": "Prediction of equivalent environments by energy exchange and assessments of physiological strain and discomfort. The described equivalence postulate was confirmed experimentally by examining physiological responses as well as discomfort sensation in a series of predicted equivalent conditions during exercise (congruent to 3 met). ET or the equivalent Ta at 50% RH is a single independent variable that is uniquely related to the mean skin temperature (Tsk), skin wettedness (w), body core temperature (Tcore) and sense of discomfort.", "PMID": 977290}
{"id": "pubmed23n0032_11065", "title": "A study of the interface between bone and acrylic cement by scanning electron microscopy.", "content": "Under the scanning electron microscope, acrylic cement presents an external surface dotted with hemispherical protuberances and a cut surface of cancellous structure. The reaction of bone to acrylic has been studied, both in stable and unstable mechanical conditions. In the first instance, the bone tissue grows towards the cement with an osteogentic front that insinuates itself between the irregularities of the external surface and tends to infiltrate the apertures in it, producing a perfect anchorage which remains solid even after a long period of time. In the second instance, the contact between cement and bone is indirect, with an interposed layer of dense fibrous tissue, probably with a shock-absorbing function.", "contents": "A study of the interface between bone and acrylic cement by scanning electron microscopy. Under the scanning electron microscope, acrylic cement presents an external surface dotted with hemispherical protuberances and a cut surface of cancellous structure. The reaction of bone to acrylic has been studied, both in stable and unstable mechanical conditions. In the first instance, the bone tissue grows towards the cement with an osteogentic front that insinuates itself between the irregularities of the external surface and tends to infiltrate the apertures in it, producing a perfect anchorage which remains solid even after a long period of time. In the second instance, the contact between cement and bone is indirect, with an interposed layer of dense fibrous tissue, probably with a shock-absorbing function.", "PMID": 977313}
{"id": "pubmed23n0032_11066", "title": "A study of the early ultrastructural changes secondary to experimental compression of the tibal nerve in rabbits.", "content": "The early ultrastructural changes which arise in nerve fibres following axonotmesis are described. The study was conducted experimentally by means of silk ligature of the tibial nerve in rabbits. The findings on electron microscopy of samples of nerve taken at two and twenty four hours respectively after operation are reported.", "contents": "A study of the early ultrastructural changes secondary to experimental compression of the tibal nerve in rabbits. The early ultrastructural changes which arise in nerve fibres following axonotmesis are described. The study was conducted experimentally by means of silk ligature of the tibial nerve in rabbits. The findings on electron microscopy of samples of nerve taken at two and twenty four hours respectively after operation are reported.", "PMID": 977314}
{"id": "pubmed23n0032_11067", "title": "Traumatic separation of the upper femoral epiphysis in a child aged two.", "content": "Traumatic separation of the proximal femoral epiphysis in children is a serious and as yet unresolved problem. Should treatment be conservative or surgical? Because of the rarity of the lesion, the literature offers no definite answer. In the case presented, conservative treatment gave a good result.", "contents": "Traumatic separation of the upper femoral epiphysis in a child aged two. Traumatic separation of the proximal femoral epiphysis in children is a serious and as yet unresolved problem. Should treatment be conservative or surgical? Because of the rarity of the lesion, the literature offers no definite answer. In the case presented, conservative treatment gave a good result.", "PMID": 977315}
{"id": "pubmed23n0032_11068", "title": "Desmoplastic fibroma of bone. (A study of three cases).", "content": "Three cases of demoplastic fibroma of bone are presented. Thirty six cases already reported in the world literature are reviewed, making the present total thirty nine cases. The extreme rarity of the tumor is emphasised, and its clinical, radiographic, morphological and pathological aspects discussed. Though the absence of metastases or histologically abnormal cells  attest to its benign nature it has a definite tendency to local recurrence. Differential diagnosis and treatment are discussed.", "contents": "Desmoplastic fibroma of bone. (A study of three cases). Three cases of demoplastic fibroma of bone are presented. Thirty six cases already reported in the world literature are reviewed, making the present total thirty nine cases. The extreme rarity of the tumor is emphasised, and its clinical, radiographic, morphological and pathological aspects discussed. Though the absence of metastases or histologically abnormal cells  attest to its benign nature it has a definite tendency to local recurrence. Differential diagnosis and treatment are discussed.", "PMID": 977316}
{"id": "pubmed23n0032_11069", "title": "A new approach to the pathology, clinical features and treatment of stress tendinopathy of the Achilles tendon.", "content": "On the basis of clinical, anatomical, surgical, histological and pathological investigations, the authors propose a classification of the tendinopathies of the Achilles tendon associated with stress. These are particularly common in the field of sport. Three syndromes are identified: a) pure peritendinitis; b) peritendinitis associated with tendinosis; c) pure tendinosis. The symptoms and possible complications are described and the problem of treatment is discussed. In pure peritendinitis tenolysis is recommended, but in peritendinitis associated with tendinosis it should be combined with extensive scarification of the tendon in order to promote revitalisation.", "contents": "A new approach to the pathology, clinical features and treatment of stress tendinopathy of the Achilles tendon. On the basis of clinical, anatomical, surgical, histological and pathological investigations, the authors propose a classification of the tendinopathies of the Achilles tendon associated with stress. These are particularly common in the field of sport. Three syndromes are identified: a) pure peritendinitis; b) peritendinitis associated with tendinosis; c) pure tendinosis. The symptoms and possible complications are described and the problem of treatment is discussed. In pure peritendinitis tenolysis is recommended, but in peritendinitis associated with tendinosis it should be combined with extensive scarification of the tendon in order to promote revitalisation.", "PMID": 977317}
{"id": "pubmed23n0032_11070", "title": "Removal of total prostheses of the hip. Review of twenty six cases.", "content": "The \"painful prosthesis\" is a difficult problem. Removal is often the best solution. Out of 566 McKee-Farrar prostheses, twenty six (4.2 per cent) were removed. The causes that made removal advisable are analysed. The methods used and the results obtained are described.", "contents": "Removal of total prostheses of the hip. Review of twenty six cases. The \"painful prosthesis\" is a difficult problem. Removal is often the best solution. Out of 566 McKee-Farrar prostheses, twenty six (4.2 per cent) were removed. The causes that made removal advisable are analysed. The methods used and the results obtained are described.", "PMID": 977318}
{"id": "pubmed23n0032_11071", "title": "Re-appraisal of treatment of congenital club foot.", "content": "Residual deformity following the treatment of club foot is still very common and in many cases it is the result of the treatment itself. The traditional treatment of this condition is undergoing a critical reappraisal with a swing towards surgery in the earlier stages. The Turco technique is discussed and the results in seventeen patients over one year of age are presented.", "contents": "Re-appraisal of treatment of congenital club foot. Residual deformity following the treatment of club foot is still very common and in many cases it is the result of the treatment itself. The traditional treatment of this condition is undergoing a critical reappraisal with a swing towards surgery in the earlier stages. The Turco technique is discussed and the results in seventeen patients over one year of age are presented.", "PMID": 977319}
{"id": "pubmed23n0032_11072", "title": "In vitro antibiotic sensitivity of Pasteurella multocida.", "content": "Thirty Pasteurella multocida strains were tested against 19 antibiotics using an agar dilution method. Penicillin G was the single most active agent tested. Anti-staphylococcal penicillins were markedly less inhibitory than penicillin G. Other penicillins including phenoxymethyl penicillin, and tetracyclines, chloramphenicol and cephalosporins all inhibited the organisms in low concentrations. Erythromycin and lincomycins had poor activity against several of the strains. The range of M.I.C.s observed for most antibiotics was narrow but wide variation was seen in zone size when antibiotic disk tests were performed.", "contents": "In vitro antibiotic sensitivity of Pasteurella multocida. Thirty Pasteurella multocida strains were tested against 19 antibiotics using an agar dilution method. Penicillin G was the single most active agent tested. Anti-staphylococcal penicillins were markedly less inhibitory than penicillin G. Other penicillins including phenoxymethyl penicillin, and tetracyclines, chloramphenicol and cephalosporins all inhibited the organisms in low concentrations. Erythromycin and lincomycins had poor activity against several of the strains. The range of M.I.C.s observed for most antibiotics was narrow but wide variation was seen in zone size when antibiotic disk tests were performed.", "PMID": 977341}
{"id": "pubmed23n0032_11073", "title": "A new medium for the presumptive identification of gram-positive uropathogens.", "content": "A significant percentage of urinary tract infections are caused by gram-positive microorganisms. The rapid identification of these uropathogens is important in determining the appropriate treatment of such infections. A selective medium containing colistin sulfate and nalidixic acid (Columbia CNA Agar) was modified by the addition of esculin, ferric ammonium citrate, mannitol and phenol red. The new medium (Esculin-Mannitol Agar) was extensively evaluated as a primary plating medium for urine specimens. Isolates were presumptively identified solely by colonial morphology and reaction of the medium. Presumptive identification of the isolates was confirmed by conventional tests. The accuracy of the presumptive identification indicated that Esculin-Mannitol Agar was useful in the primary plating of urine specimens when employed together with an appropriate medium for the recovery of gram-negative organisms.", "contents": "A new medium for the presumptive identification of gram-positive uropathogens. A significant percentage of urinary tract infections are caused by gram-positive microorganisms. The rapid identification of these uropathogens is important in determining the appropriate treatment of such infections. A selective medium containing colistin sulfate and nalidixic acid (Columbia CNA Agar) was modified by the addition of esculin, ferric ammonium citrate, mannitol and phenol red. The new medium (Esculin-Mannitol Agar) was extensively evaluated as a primary plating medium for urine specimens. Isolates were presumptively identified solely by colonial morphology and reaction of the medium. Presumptive identification of the isolates was confirmed by conventional tests. The accuracy of the presumptive identification indicated that Esculin-Mannitol Agar was useful in the primary plating of urine specimens when employed together with an appropriate medium for the recovery of gram-negative organisms.", "PMID": 977342}
{"id": "pubmed23n0032_11074", "title": "The antimicrobial activity of embalming chemicals and topical disinfectants on the microbial flora of human remains.", "content": "The antimicrobial activity of embalming chemicals an topical disinfectants was evaluated to determine the degree of disinfection achieved during the embalming of human remains. The administration of arterial and cavity embalming chemicals resulted in a 99% reduction of the postmortem microbial population after 2 hours of contact. This level of disinfection was maintained for the 24 hours test period. Topical disinfection of the body orifices was also observed. Therefore, it is probable that present embalming practices reduce the hazard from transmission of potentially infectious microbial agents within the immediate environment of embalmed human remains.", "contents": "The antimicrobial activity of embalming chemicals and topical disinfectants on the microbial flora of human remains. The antimicrobial activity of embalming chemicals an topical disinfectants was evaluated to determine the degree of disinfection achieved during the embalming of human remains. The administration of arterial and cavity embalming chemicals resulted in a 99% reduction of the postmortem microbial population after 2 hours of contact. This level of disinfection was maintained for the 24 hours test period. Topical disinfection of the body orifices was also observed. Therefore, it is probable that present embalming practices reduce the hazard from transmission of potentially infectious microbial agents within the immediate environment of embalmed human remains.", "PMID": 977343}
{"id": "pubmed23n0032_11075", "title": "Seattle-King County revisited.", "content": "For approximately five years the Seattle-King County Public Health Laboratory has measured its diagnostic laboratory workload quantitatively in terms of relative values. Seattle-King County has used the laboratory average cost-per-relative value concept. However, increasing operational costs, multiple demands for the same tax dollar, and professional desire for more precise costing information led the laboratory staff to refine their method of determining a cost-per-relative value. This article shares their new methodology of determining a cost-per-relative value for each primary cost center. Also shared are a few examples of how costs differ when using the old method in comparison to using the new method.", "contents": "Seattle-King County revisited. For approximately five years the Seattle-King County Public Health Laboratory has measured its diagnostic laboratory workload quantitatively in terms of relative values. Seattle-King County has used the laboratory average cost-per-relative value concept. However, increasing operational costs, multiple demands for the same tax dollar, and professional desire for more precise costing information led the laboratory staff to refine their method of determining a cost-per-relative value. This article shares their new methodology of determining a cost-per-relative value for each primary cost center. Also shared are a few examples of how costs differ when using the old method in comparison to using the new method.", "PMID": 977344}
{"id": "pubmed23n0032_11076", "title": "The dentist and preventive dental health information.", "content": "Patients receive differing amounts of preventive dental health information from their dentist. What factors affect the amount of preventive information a patient receives? Many possible reasons which have been advanced for this difference are discussed in this paper. Using the clues presented in the literature, and results of a presurvey, some factors thought to influence the amount of preventive dental health information the patient receives were investigated. Specifically, the research problem was, \"What is the relationship between the amount of preventive dental health information a patient receives from the dentist in his private office and the following factors: patient's appearance, interest-responsiveness, nervousness, need for dental care, request for service, orientation to dentistry, and dentist's liking for the patient?\" The hypothesized relationships of amount of preventive dental information delivered to dentist's perception of patient's characteristics were not upheld. The paper tentatively concludes that the characteristics of the dentist and his dental practice affect the preventive information delivered more significantly than patient characteristics.", "contents": "The dentist and preventive dental health information. Patients receive differing amounts of preventive dental health information from their dentist. What factors affect the amount of preventive information a patient receives? Many possible reasons which have been advanced for this difference are discussed in this paper. Using the clues presented in the literature, and results of a presurvey, some factors thought to influence the amount of preventive dental health information the patient receives were investigated. Specifically, the research problem was, \"What is the relationship between the amount of preventive dental health information a patient receives from the dentist in his private office and the following factors: patient's appearance, interest-responsiveness, nervousness, need for dental care, request for service, orientation to dentistry, and dentist's liking for the patient?\" The hypothesized relationships of amount of preventive dental information delivered to dentist's perception of patient's characteristics were not upheld. The paper tentatively concludes that the characteristics of the dentist and his dental practice affect the preventive information delivered more significantly than patient characteristics.", "PMID": 977352}
{"id": "pubmed23n0032_11077", "title": "Health education manpower in the United States.", "content": "Problems in collection of uniform data on health education manpower on a continuing basis are discussed. Sources of data on current health education manpower projections for future needs are reviewed. Possible directions for improving manpower planning in this field are cited.", "contents": "Health education manpower in the United States. Problems in collection of uniform data on health education manpower on a continuing basis are discussed. Sources of data on current health education manpower projections for future needs are reviewed. Possible directions for improving manpower planning in this field are cited.", "PMID": 977353}
{"id": "pubmed23n0032_11078", "title": "Changes in the activities, functions, and roles of public health educators.", "content": "Accounts of early activities of public health educators, statements of the American Public Health Association on the qualifications and functions of these educators, and studies concerned with their responsbiliities, functions, work, or roles are reviewed. These point up the three major foci in public health education over time in the U.S., viz, dissemination of information, community organization, and health behavior and program planning. Functions of public health educators in emerging settings for practice are presented and the implications of this movement (i.e., movement of health educators into non-traditional settings) for public health education profession are discussed.", "contents": "Changes in the activities, functions, and roles of public health educators. Accounts of early activities of public health educators, statements of the American Public Health Association on the qualifications and functions of these educators, and studies concerned with their responsbiliities, functions, work, or roles are reviewed. These point up the three major foci in public health education over time in the U.S., viz, dissemination of information, community organization, and health behavior and program planning. Functions of public health educators in emerging settings for practice are presented and the implications of this movement (i.e., movement of health educators into non-traditional settings) for public health education profession are discussed.", "PMID": 977354}
{"id": "pubmed23n0032_11079", "title": "Preparation of manpower for health education: a comparative view.", "content": "Differences between various models in the organization of health education services are explored. New developments in health education approaches used in training, career structures, and job definitions in some European countries are summarized. Problem areas have been defined and recommendations have been produced by numerous activities of the World Health Organization in recent years. At a 1974 symposium, it became obvious that no planned manpower development is feasible without a job definition of the health educator as an educational product. The need to specify the aims of future developments requires taking a critical view of past developments and spelling out existing differences.", "contents": "Preparation of manpower for health education: a comparative view. Differences between various models in the organization of health education services are explored. New developments in health education approaches used in training, career structures, and job definitions in some European countries are summarized. Problem areas have been defined and recommendations have been produced by numerous activities of the World Health Organization in recent years. At a 1974 symposium, it became obvious that no planned manpower development is feasible without a job definition of the health educator as an educational product. The need to specify the aims of future developments requires taking a critical view of past developments and spelling out existing differences.", "PMID": 977356}
{"id": "pubmed23n0032_11080", "title": "The role of the clinical health educator.", "content": "A combination of forces favor the expansion of health education efforts in hospitals. This article describes four areas of health education in clinical settings: the justification for a health educator in this setting; his preparation; his role as a member of the health care team; and the extended role of the educator in the community, with voluntary and official agencies, and with families of patients.", "contents": "The role of the clinical health educator. A combination of forces favor the expansion of health education efforts in hospitals. This article describes four areas of health education in clinical settings: the justification for a health educator in this setting; his preparation; his role as a member of the health care team; and the extended role of the educator in the community, with voluntary and official agencies, and with families of patients.", "PMID": 977357}
{"id": "pubmed23n0032_11081", "title": "[Psychiatric consultation in pediatrics. Description and problems].", "content": "In this study of psychiatric consultations in a paediatric department, a description is given of the evolution and duration of treatment in the first 55 cases seen. Children ranged from age 1 month to 12 years. Two findings are of particular interest: most of the children were of a very young age (less than 3 years); most psychiatric treatments were of short duration. These results are due to the preventive orientation of our consultation and to the close collaboration between pediatricians and psychiatrists. The preventive approach allows for less heavy and costly measures than the standard long-term psychiatric treatment. This preventive approach is only possible if the diagnosis is established early. The pediatrician is in a better position than the psychiatrist to practice early detection, as in young children the psychopathology is often expressed through somatic channels. In cases where the pathology was detected at a later age, the treatment is usually more difficult.", "contents": "[Psychiatric consultation in pediatrics. Description and problems]. In this study of psychiatric consultations in a paediatric department, a description is given of the evolution and duration of treatment in the first 55 cases seen. Children ranged from age 1 month to 12 years. Two findings are of particular interest: most of the children were of a very young age (less than 3 years); most psychiatric treatments were of short duration. These results are due to the preventive orientation of our consultation and to the close collaboration between pediatricians and psychiatrists. The preventive approach allows for less heavy and costly measures than the standard long-term psychiatric treatment. This preventive approach is only possible if the diagnosis is established early. The pediatrician is in a better position than the psychiatrist to practice early detection, as in young children the psychopathology is often expressed through somatic channels. In cases where the pathology was detected at a later age, the treatment is usually more difficult.", "PMID": 977371}
{"id": "pubmed23n0032_11082", "title": "[Brain tumors in childhood. Clinical aspects].", "content": "The analysis of 76 case histories of children suffering from brain tumor revealed a majority in preschool age. The diagnosis at admission to the hospital was correct in 2/3 of the cases. The main causes for admission among unclear cases were headache, a history of head trauma, and epilepsy. An evaluation is made of the diagnostic value of symptoms, neurological signs, and of auxiliary investigations.", "contents": "[Brain tumors in childhood. Clinical aspects]. The analysis of 76 case histories of children suffering from brain tumor revealed a majority in preschool age. The diagnosis at admission to the hospital was correct in 2/3 of the cases. The main causes for admission among unclear cases were headache, a history of head trauma, and epilepsy. An evaluation is made of the diagnostic value of symptoms, neurological signs, and of auxiliary investigations.", "PMID": 977372}
{"id": "pubmed23n0032_11083", "title": "[Genetics of idiopathic scoliosis].", "content": "Families of 241 patients among 913 cases of idiopathic scoliosis were surveyed. The data suggest two possible modes of inheritance: either an autosomal dominant inheritance with incomplete penetrance and more frequent manifestation in girls than boys, or a genetic heterogeneity with a mixture of dominant and multifactorial modes of inheritance.", "contents": "[Genetics of idiopathic scoliosis]. Families of 241 patients among 913 cases of idiopathic scoliosis were surveyed. The data suggest two possible modes of inheritance: either an autosomal dominant inheritance with incomplete penetrance and more frequent manifestation in girls than boys, or a genetic heterogeneity with a mixture of dominant and multifactorial modes of inheritance.", "PMID": 977374}
{"id": "pubmed23n0032_11084", "title": "[Influence of ouabain on the electrophoretic mobility of erythrocytes in 7 patients with Duchenne's muscular dystrophy].", "content": "Measurements of electrophoretic mobility of erythrocytes under the influence of ouabain were carried out on seven patients suffering from progressive Duchenne's muscular dystrophy. While ouabain-treated erythrocytes of healthy controls showed a significant decrease (alpha = 0.001) in electrophoretic mobility, an increase was found in five of the patients, and two patients revealed a slight drop in electrophoretic mobility. A significant difference (alpha = 0.001) was obtained in comparing the control group of patients. Initial trial measurements suggested that such a difference is also true for some other forms of muscular dystrophy and Werdnig-Hoffmann's spinal muscular atrophy.", "contents": "[Influence of ouabain on the electrophoretic mobility of erythrocytes in 7 patients with Duchenne's muscular dystrophy]. Measurements of electrophoretic mobility of erythrocytes under the influence of ouabain were carried out on seven patients suffering from progressive Duchenne's muscular dystrophy. While ouabain-treated erythrocytes of healthy controls showed a significant decrease (alpha = 0.001) in electrophoretic mobility, an increase was found in five of the patients, and two patients revealed a slight drop in electrophoretic mobility. A significant difference (alpha = 0.001) was obtained in comparing the control group of patients. Initial trial measurements suggested that such a difference is also true for some other forms of muscular dystrophy and Werdnig-Hoffmann's spinal muscular atrophy.", "PMID": 977375}
{"id": "pubmed23n0032_11085", "title": "Cardiac complications after vaccination for smallpox.", "content": "A case of severe myocardial damage 8 days after smallpox vaccination is described in a 6-year-old boy. A huge embolus overriding the bifurcation of the aorta complicated the disease and was removed surgically. Attention is called to the rapid recovery of this patient, which may be attributed to the use of massive doses of antivaccinia gamma globulin.", "contents": "Cardiac complications after vaccination for smallpox. A case of severe myocardial damage 8 days after smallpox vaccination is described in a 6-year-old boy. A huge embolus overriding the bifurcation of the aorta complicated the disease and was removed surgically. Attention is called to the rapid recovery of this patient, which may be attributed to the use of massive doses of antivaccinia gamma globulin.", "PMID": 977376}
{"id": "pubmed23n0032_11086", "title": "Cricopharyngeal achalasia associated with congenital suprabulbar paresis.", "content": "A case of congenital suprabulbar paresis is reported. The associated severe dysphagia which presented soon after birth was shown by radiographic and cineradiographic studies to be due to cricopharyngeal achalasia. During four years of follow-up a clear tendency to spontaneous improvement was seen. It is therefore suggested that the condition may have a favorable outcome without resorting to surgical intervention.", "contents": "Cricopharyngeal achalasia associated with congenital suprabulbar paresis. A case of congenital suprabulbar paresis is reported. The associated severe dysphagia which presented soon after birth was shown by radiographic and cineradiographic studies to be due to cricopharyngeal achalasia. During four years of follow-up a clear tendency to spontaneous improvement was seen. It is therefore suggested that the condition may have a favorable outcome without resorting to surgical intervention.", "PMID": 977377}
{"id": "pubmed23n0032_11087", "title": "[The ultrastructure of dyskeratotic and dysplastic keratinocytes in oral epithelium (author's transl)].", "content": "The fine structural morphology of dyskeratotic and dysplastic keratinocytes in human oral epithelium was investigated by light microscopy as well as by transmission and scanning-electron microscopy. On the epithelial-connective tissue border, marked changes are seen in the form of polymorphous microinvasive cytoplasmic processes of basal keratinocytes, structural alterations of the basement membrane and rarefaction of the subepithelial connective tissue. Dyskeratotic keratinocytes with abnormal tonofilament configuration are phagocytized by dermal macrophages and are transported to the lamina propria. Ultrastructural signs of atypia are found in the nucleus, nucleolus, cytoplasmic organelles and mitotic apparatus. Furthermore, multiple alterations of the plasma membrane, decrease in numbers of junctional complexes and acantholytic widened intercellular spaces are observed. Intracytoplasmic lumina are formed by endocytotic invagination of desmosome-studed plasma membrane regions at the cell surface. Despite an inverse relationship between the degree of keratinization and the glycogen content of the epithelium at the subcellular level, large amounts of glycogen are found in some keratinocytes. The epithelial surface is formed by hyper-, para-, or orthokeratosis, or shows individual cell keratinization, alteration of the disintegration process and defective keratin synthesis. The ultrastructural analysis of dysplastic keratinocyte populations reveals some morphological criteria common with invasive squamous cell carcinoma, which may be important in the early diagnosis and prognosis of malignant transformation of epithelial dysplasia.", "contents": "[The ultrastructure of dyskeratotic and dysplastic keratinocytes in oral epithelium (author's transl)]. The fine structural morphology of dyskeratotic and dysplastic keratinocytes in human oral epithelium was investigated by light microscopy as well as by transmission and scanning-electron microscopy. On the epithelial-connective tissue border, marked changes are seen in the form of polymorphous microinvasive cytoplasmic processes of basal keratinocytes, structural alterations of the basement membrane and rarefaction of the subepithelial connective tissue. Dyskeratotic keratinocytes with abnormal tonofilament configuration are phagocytized by dermal macrophages and are transported to the lamina propria. Ultrastructural signs of atypia are found in the nucleus, nucleolus, cytoplasmic organelles and mitotic apparatus. Furthermore, multiple alterations of the plasma membrane, decrease in numbers of junctional complexes and acantholytic widened intercellular spaces are observed. Intracytoplasmic lumina are formed by endocytotic invagination of desmosome-studed plasma membrane regions at the cell surface. Despite an inverse relationship between the degree of keratinization and the glycogen content of the epithelium at the subcellular level, large amounts of glycogen are found in some keratinocytes. The epithelial surface is formed by hyper-, para-, or orthokeratosis, or shows individual cell keratinization, alteration of the disintegration process and defective keratin synthesis. The ultrastructural analysis of dysplastic keratinocyte populations reveals some morphological criteria common with invasive squamous cell carcinoma, which may be important in the early diagnosis and prognosis of malignant transformation of epithelial dysplasia.", "PMID": 977387}
{"id": "pubmed23n0032_11088", "title": "[Diagnostic importance of electrophysiologic tests in Bell's palsy: report on a longterm follow-up study in 100 cases (author's transl)].", "content": "The present study is based upon the results of repeated electrodiagnostic tests carried out in a series of 100 cases of Bell's palsy (Determination of Rheobase and Chronaxy). The first symptoms of denervation can be observed during the second week after the onset of the paralysis. This seems to be the critical period in which the ultimate consequences of the lesion are determined: partial or total recovery or complete irreversible denervation. In cases showing a rapid increase of chronaxy during the 2nd week, prognosis is rather poor because a difference of 240% between the affected and the normal side indicates a progressive denervation. Cases in which the difference of chronaxy values during the 2nd week is increased upto 240% (i.e. partial denervation) recover incompletely in 15% of the cases. Estimation of prognosis in Bell's palsy and timing of a facial nerve decompression must be based on reliable facts, such as the results of repeated electrodiagnostic tests (modified measurement of Rheobase and Chronaxy) carried out at least every secont but preferably every day during the first 3 weeks after the onset of the paresis.", "contents": "[Diagnostic importance of electrophysiologic tests in Bell's palsy: report on a longterm follow-up study in 100 cases (author's transl)]. The present study is based upon the results of repeated electrodiagnostic tests carried out in a series of 100 cases of Bell's palsy (Determination of Rheobase and Chronaxy). The first symptoms of denervation can be observed during the second week after the onset of the paralysis. This seems to be the critical period in which the ultimate consequences of the lesion are determined: partial or total recovery or complete irreversible denervation. In cases showing a rapid increase of chronaxy during the 2nd week, prognosis is rather poor because a difference of 240% between the affected and the normal side indicates a progressive denervation. Cases in which the difference of chronaxy values during the 2nd week is increased upto 240% (i.e. partial denervation) recover incompletely in 15% of the cases. Estimation of prognosis in Bell's palsy and timing of a facial nerve decompression must be based on reliable facts, such as the results of repeated electrodiagnostic tests (modified measurement of Rheobase and Chronaxy) carried out at least every secont but preferably every day during the first 3 weeks after the onset of the paresis.", "PMID": 977388}
{"id": "pubmed23n0032_11089", "title": "[An intralabyrinthine and intratympanic acoustic neuroma (author's transl)].", "content": "An intra labyrinthine acoustic neuroma, which had simulated otitis media, is reported. The tumour had extended into the cavum tympani over the basal turn of the cochlea and into all the seimicircular canals. The bulk of the tumour lay in a wide defect between the vestibule and the fundus of the internal auditory canal. The literature on intralabyrinthine neuromas is reviewed.", "contents": "[An intralabyrinthine and intratympanic acoustic neuroma (author's transl)]. An intra labyrinthine acoustic neuroma, which had simulated otitis media, is reported. The tumour had extended into the cavum tympani over the basal turn of the cochlea and into all the seimicircular canals. The bulk of the tumour lay in a wide defect between the vestibule and the fundus of the internal auditory canal. The literature on intralabyrinthine neuromas is reviewed.", "PMID": 977389}
{"id": "pubmed23n0032_11090", "title": "[Applied anatomy of the facial nerve. I. Nuclei, supranuclear connectiions and peripheral nerve (author's transl)].", "content": "The clinically important anatomy and morphology of the facial nerve is reviewed. The nuclei, and the supranuclear and internuclear connections are described, and the facial canal, its dehiscences and its relation with the nerve are discussed in detail.", "contents": "[Applied anatomy of the facial nerve. I. Nuclei, supranuclear connectiions and peripheral nerve (author's transl)]. The clinically important anatomy and morphology of the facial nerve is reviewed. The nuclei, and the supranuclear and internuclear connections are described, and the facial canal, its dehiscences and its relation with the nerve are discussed in detail.", "PMID": 977393}
{"id": "pubmed23n0032_11091", "title": "[Hazards associated with artificial humidification in tracheotomized patients (author's transl)].", "content": "Bacteriological testing in tracheotomized patients has shown that infection can be avoided when only sterilized water is used for post-surgical humidification and is disinfected at least every two days. In so doing, water condensation can be avoided by the use of concomitant tube heating. Conclusions indicate that humidifers which utilize water evaporation are more useful in patient care than ultra-sonic nebulizers.", "contents": "[Hazards associated with artificial humidification in tracheotomized patients (author's transl)]. Bacteriological testing in tracheotomized patients has shown that infection can be avoided when only sterilized water is used for post-surgical humidification and is disinfected at least every two days. In so doing, water condensation can be avoided by the use of concomitant tube heating. Conclusions indicate that humidifers which utilize water evaporation are more useful in patient care than ultra-sonic nebulizers.", "PMID": 977394}
{"id": "pubmed23n0032_11092", "title": "[Cervical-nystagmus as related to vertebral receptors].", "content": "Two patients with absent peripheral labyrinthine function are reported, in whome cervical nystagmus could be demonstrated. Return of labyrinthine function with simultaneous disappearance of the cervical nystagmus in one of the patients demonstrates existence of receptors in the upper three vertebral joints and their potential influence on the vestibular system. Pathogenetic as well as diagnostic functions of the sensory afferent systems of the upper cervical region are discussed.", "contents": "[Cervical-nystagmus as related to vertebral receptors]. Two patients with absent peripheral labyrinthine function are reported, in whome cervical nystagmus could be demonstrated. Return of labyrinthine function with simultaneous disappearance of the cervical nystagmus in one of the patients demonstrates existence of receptors in the upper three vertebral joints and their potential influence on the vestibular system. Pathogenetic as well as diagnostic functions of the sensory afferent systems of the upper cervical region are discussed.", "PMID": 977395}
{"id": "pubmed23n0032_11093", "title": "[Endo-ecto-laryngeal dysproportion: a frequent cause of dysplastic dysphonia (author's transl)].", "content": "A post-mutational laryngeal dysplasia is described which results in rapid vocal fatigue and chronic dysphonia. Pathognostically important in the respiratory position is a profile-deficient endolarynx, combined with hypoplastic Morgagni ventricle and distinct vocal cord contour in the phonatory position. We interpret this dysplasia as the consequence of a dysproportionate growth process during which the growth of the endolaryngeal soft tissue fails to match that of the laryngeal frame.", "contents": "[Endo-ecto-laryngeal dysproportion: a frequent cause of dysplastic dysphonia (author's transl)]. A post-mutational laryngeal dysplasia is described which results in rapid vocal fatigue and chronic dysphonia. Pathognostically important in the respiratory position is a profile-deficient endolarynx, combined with hypoplastic Morgagni ventricle and distinct vocal cord contour in the phonatory position. We interpret this dysplasia as the consequence of a dysproportionate growth process during which the growth of the endolaryngeal soft tissue fails to match that of the laryngeal frame.", "PMID": 977396}
{"id": "pubmed23n0032_11094", "title": "[Combined treatment of a cicatrised post-intubation complication with surgery and medication summary (author's transl)].", "content": "A case of postintubation laryngotracheal injury is described. The paramedian position of the vocal cords was found to be a consequence of cicatrix in the area of the posterior commissure, and by a luxation with ankylosis of the right cricoarytenoid joint. Paresis of the recurrent laryngeal nerve was excluded by electromyography. Multiple incisions of the cicatrix failed to correct the problem, whereas subsequent incision followed by intensive therapy with fludrocortisone and hyaluronidase was successful in management. In administering the medication, a new simplifed method for translaryngeal injection is described.", "contents": "[Combined treatment of a cicatrised post-intubation complication with surgery and medication summary (author's transl)]. A case of postintubation laryngotracheal injury is described. The paramedian position of the vocal cords was found to be a consequence of cicatrix in the area of the posterior commissure, and by a luxation with ankylosis of the right cricoarytenoid joint. Paresis of the recurrent laryngeal nerve was excluded by electromyography. Multiple incisions of the cicatrix failed to correct the problem, whereas subsequent incision followed by intensive therapy with fludrocortisone and hyaluronidase was successful in management. In administering the medication, a new simplifed method for translaryngeal injection is described.", "PMID": 977397}
{"id": "pubmed23n0032_11095", "title": "[Prolonged endotracheal intubation or tracheostomy in children (author's transl)].", "content": "Five patients with subglottic tracheal stenosis following prolonged endotracheal intubation are reported. To minimize tracheal stenosis the indications for prolonged intubation should be well defined and tracheostomy considered as an alternative. The incidence of tracheal stenosis following prolonged intubation is stimilar to that following tracheostomy. The risk of stenosis increases with the duration of intubation, the degree of physical trauma to the laryngotracheal mucosa (suction, tube changing, restlessness), infection of the trachea or larynx, and with the age of the child. Prolonged intubation necessitates sedation and intensive care. Tracheostomy has a higher mortality but this and the risk of stenosis depend greatly on the operative technique. Particularly in cases where prolonged intubation increase the risk of tracheal stenosis, the advantages of tracheotomy become evident. Tracheostomised children rarely need sedation, the tracheobronchial tree can be easily and carefully toileted and the changing of the tube is without risk. Neither method is absolutely preferable, but the correct application of both will minimise the complication rate. The indications for each may be summarised as follows: for primary treatment of acute respiratory distress in children prolonged intubation is the treatment of choice. If after 3 days there is no chance of extubation, tracheostomy should be considered but this depends also on the child's age and behaviour, and on the laryngotracheal mucosal reaction. The younger the child the more cautiously should tracheostomy be considered. Children under 2 years of age should only be tracheostomised if there is no alternative.", "contents": "[Prolonged endotracheal intubation or tracheostomy in children (author's transl)]. Five patients with subglottic tracheal stenosis following prolonged endotracheal intubation are reported. To minimize tracheal stenosis the indications for prolonged intubation should be well defined and tracheostomy considered as an alternative. The incidence of tracheal stenosis following prolonged intubation is stimilar to that following tracheostomy. The risk of stenosis increases with the duration of intubation, the degree of physical trauma to the laryngotracheal mucosa (suction, tube changing, restlessness), infection of the trachea or larynx, and with the age of the child. Prolonged intubation necessitates sedation and intensive care. Tracheostomy has a higher mortality but this and the risk of stenosis depend greatly on the operative technique. Particularly in cases where prolonged intubation increase the risk of tracheal stenosis, the advantages of tracheotomy become evident. Tracheostomised children rarely need sedation, the tracheobronchial tree can be easily and carefully toileted and the changing of the tube is without risk. Neither method is absolutely preferable, but the correct application of both will minimise the complication rate. The indications for each may be summarised as follows: for primary treatment of acute respiratory distress in children prolonged intubation is the treatment of choice. If after 3 days there is no chance of extubation, tracheostomy should be considered but this depends also on the child's age and behaviour, and on the laryngotracheal mucosal reaction. The younger the child the more cautiously should tracheostomy be considered. Children under 2 years of age should only be tracheostomised if there is no alternative.", "PMID": 977398}
{"id": "pubmed23n0032_11096", "title": "Comparison of acoustic reflex and behavioral thresholds as a function of stimulus frequency and duration.", "content": "Threshold-duration functions for the acoustic reflex and threshold-duration functions based upon behavioral measures were obtained on the same group of subjects and compared. The mean temporal integration for the acoustic reflex threshold appears to be comparable to that for the behavioral threshold for stimuli of 500, 1000, and 2000 Hz, but marked individual differences exist in temporal integration of the acoustic reflex. At 4000 Hz, the mean change in the reflex threshold as a function of stimulus duration is significantly greater than the change in the behavioral threshold under the same conditions, suggesting that in the elicitation of the acoustic reflex the auditory system processes energy less efficiently at 4000 Hz than it does at lower frequencies.", "contents": "Comparison of acoustic reflex and behavioral thresholds as a function of stimulus frequency and duration. Threshold-duration functions for the acoustic reflex and threshold-duration functions based upon behavioral measures were obtained on the same group of subjects and compared. The mean temporal integration for the acoustic reflex threshold appears to be comparable to that for the behavioral threshold for stimuli of 500, 1000, and 2000 Hz, but marked individual differences exist in temporal integration of the acoustic reflex. At 4000 Hz, the mean change in the reflex threshold as a function of stimulus duration is significantly greater than the change in the behavioral threshold under the same conditions, suggesting that in the elicitation of the acoustic reflex the auditory system processes energy less efficiently at 4000 Hz than it does at lower frequencies.", "PMID": 977428}
{"id": "pubmed23n0032_11097", "title": "Perception of time-compressed consonant-nucleus-consonant monosyllables by non-native speaker/listeners of English.", "content": "Recent investigations of time-altered speech have dealt with the effect of time compression and sensation level on intelligibility scores of native speaker/listeners of English. In the present investigation, the intelligibility of time-compressed consonant-nucleus-consonant monosyllables was studied using English speaker/listeners whose native languages are Spanish or Indo-Dravidian. Results supported earlier findings in that intelligibility decreased as a function of increasing percentage of time compression and decreasing sensation level. This effect was more prominent for the Indo-Dravidian than for Spanish speaker/listeners. The Spanish group of subjects showed generally lower difference scores than did the Indo-Dravidian group when compared to native English speaker/listeners.", "contents": "Perception of time-compressed consonant-nucleus-consonant monosyllables by non-native speaker/listeners of English. Recent investigations of time-altered speech have dealt with the effect of time compression and sensation level on intelligibility scores of native speaker/listeners of English. In the present investigation, the intelligibility of time-compressed consonant-nucleus-consonant monosyllables was studied using English speaker/listeners whose native languages are Spanish or Indo-Dravidian. Results supported earlier findings in that intelligibility decreased as a function of increasing percentage of time compression and decreasing sensation level. This effect was more prominent for the Indo-Dravidian than for Spanish speaker/listeners. The Spanish group of subjects showed generally lower difference scores than did the Indo-Dravidian group when compared to native English speaker/listeners.", "PMID": 977429}
{"id": "pubmed23n0032_11098", "title": "Modified rhyme test and synthetic sentence identification test scores of normal and hearing-impaired subjects listening in multitalker noise.", "content": "Articulation functions were established for 35 normal hearing, 6 flat sensorineural, and 6 high frequency sloping sensorineural hearing-impaired subjects using the modified rhyme test (MRT) and synthetic sentence identification test (SSIT) in three message competition ratios (MCRs) of multitalker noise. An analysis of variance revealed significant differences between tests, subject groups, presentation conditions, and interactions between variables. Post hoc analyses revealed that SSIT functions were not significantly different among groups, and that MRT functions were different for each group. The MRT and SSIT were found to be two significantly different tests, each taxing different areas of discrimination. Results suggest that both tests can be used clinically with various MCRs of multitalker noise, but that the poorest MCR for each test does not aid in defining discrimination abilities.", "contents": "Modified rhyme test and synthetic sentence identification test scores of normal and hearing-impaired subjects listening in multitalker noise. Articulation functions were established for 35 normal hearing, 6 flat sensorineural, and 6 high frequency sloping sensorineural hearing-impaired subjects using the modified rhyme test (MRT) and synthetic sentence identification test (SSIT) in three message competition ratios (MCRs) of multitalker noise. An analysis of variance revealed significant differences between tests, subject groups, presentation conditions, and interactions between variables. Post hoc analyses revealed that SSIT functions were not significantly different among groups, and that MRT functions were different for each group. The MRT and SSIT were found to be two significantly different tests, each taxing different areas of discrimination. Results suggest that both tests can be used clinically with various MCRs of multitalker noise, but that the poorest MCR for each test does not aid in defining discrimination abilities.", "PMID": 977431}
{"id": "pubmed23n0032_11099", "title": "Surgical repair of atrial septal defect in a dog.", "content": "Atrial septal defect, a relatively rare canine congenital cardiovascular defect, caused syncope during excitement in a 5-year-old male Boxer dog. The defect was successfully repaired by open heart surgery.", "contents": "Surgical repair of atrial septal defect in a dog. Atrial septal defect, a relatively rare canine congenital cardiovascular defect, caused syncope during excitement in a 5-year-old male Boxer dog. The defect was successfully repaired by open heart surgery.", "PMID": 977437}
{"id": "pubmed23n0032_11100", "title": "Traumatically induced bullous lung lesions in the dog: a radiographic report of three cases.", "content": "Three dogs with automobile-induced trauma had radiographic evidence of multiple bullous pulmonary lesions. Two of these dogs were subsequently reexamined, and resolution of their lesions was demonstrated radiographically.", "contents": "Traumatically induced bullous lung lesions in the dog: a radiographic report of three cases. Three dogs with automobile-induced trauma had radiographic evidence of multiple bullous pulmonary lesions. Two of these dogs were subsequently reexamined, and resolution of their lesions was demonstrated radiographically.", "PMID": 977438}
{"id": "pubmed23n0032_11101", "title": "Study of the classical and modified alternate binaural loudness balance tests in normal and pathological ears.", "content": "This study was carried out to examine recruitment of loudness measured by the classical and the modified, self-recording alternate binaural loudness balance tests in 20 normal subjects and in 14 patients with various kinds of ear pathology. In spite of individual variability, the modified method, when used as described in this study, does provide an indication of the presence or absence of loudness recruitment.", "contents": "Study of the classical and modified alternate binaural loudness balance tests in normal and pathological ears. This study was carried out to examine recruitment of loudness measured by the classical and the modified, self-recording alternate binaural loudness balance tests in 20 normal subjects and in 14 patients with various kinds of ear pathology. In spite of individual variability, the modified method, when used as described in this study, does provide an indication of the presence or absence of loudness recruitment.", "PMID": 977430}
{"id": "pubmed23n0032_11102", "title": "Evaluation of monensin toxicity in the horse.", "content": "Gelded horses of mixed breeding, ranging in weight between 360 and 455 kg, were fed pellets that contained monensin at concentrations of 279, 124, 31, and 0 ppm. Of 2 horses fed 279 ppm (253 g/T), both died. Of 3 horses fed 125 ppm (113 g/T), 1 died; feed intake was reduced in the 2 survivors. Of 3 horses fed 31 ppm (28 g/T), 1 had a transitory reduction in feed intake and the other 2 remained clinically normal. The 2 horses fed 0 ppm remained clinically normal. Monensin was also given to horses by gavage, at dosages of 1, 2, 3, 4, or 20 mg/kg of body weight. The LD50 was estimated to be between 2 and 3 mg/kg of body weight. The signs of toxicosis were partial to complete anorexia, ataxia, and intermittent profuse sweating.", "contents": "Evaluation of monensin toxicity in the horse. Gelded horses of mixed breeding, ranging in weight between 360 and 455 kg, were fed pellets that contained monensin at concentrations of 279, 124, 31, and 0 ppm. Of 2 horses fed 279 ppm (253 g/T), both died. Of 3 horses fed 125 ppm (113 g/T), 1 died; feed intake was reduced in the 2 survivors. Of 3 horses fed 31 ppm (28 g/T), 1 had a transitory reduction in feed intake and the other 2 remained clinically normal. The 2 horses fed 0 ppm remained clinically normal. Monensin was also given to horses by gavage, at dosages of 1, 2, 3, 4, or 20 mg/kg of body weight. The LD50 was estimated to be between 2 and 3 mg/kg of body weight. The signs of toxicosis were partial to complete anorexia, ataxia, and intermittent profuse sweating.", "PMID": 977440}
{"id": "pubmed23n0032_11103", "title": "Naturally occurring and experimentally induced eastern encephalomyelitis in calves.", "content": "Eastern encephalomyelitis virus was isolated from the brain of 2 calves with encephalomyelitis. Using the isolant from 1 of these calves, the disease was reproduced in a clinically normal calf. Histopathologic features conformed with those described for eastern encephalomyelitis in the horse.", "contents": "Naturally occurring and experimentally induced eastern encephalomyelitis in calves. Eastern encephalomyelitis virus was isolated from the brain of 2 calves with encephalomyelitis. Using the isolant from 1 of these calves, the disease was reproduced in a clinically normal calf. Histopathologic features conformed with those described for eastern encephalomyelitis in the horse.", "PMID": 977441}
{"id": "pubmed23n0032_11104", "title": "Coagulopathy and encephalopathy in a dog with acute hepatic necrosis.", "content": "Disseminated intravascular coagulation developed secondary to hepatic necrosis in a 5-year-old Saint Bernard. Although the coagulopathy responded to treatment with heparin, the dog died from the combined effects of gastric hemorrhage and encephalopathy, both of which are complications of hepatic necrosis.", "contents": "Coagulopathy and encephalopathy in a dog with acute hepatic necrosis. Disseminated intravascular coagulation developed secondary to hepatic necrosis in a 5-year-old Saint Bernard. Although the coagulopathy responded to treatment with heparin, the dog died from the combined effects of gastric hemorrhage and encephalopathy, both of which are complications of hepatic necrosis.", "PMID": 977448}
{"id": "pubmed23n0032_11105", "title": "A muscle disorder of Labrador retrievers characterized by deficiency of type II muscle fibers.", "content": "A muscle disorder was detected in 5 Labrador Retrievers. The age of onset was less than 6 months. The clinical signs, which were exaggerated with exercise, cold, or excitement, included abnormal head and neck posture, and a stiff, forced, hopping gait. A marked deficiency of skeletal muscle mass resulted in poor conformation. Both black and yellow Labrador Retrievers were affected, and pedigree studies indicated the disorder may have been inherited as an autosomal recessive trait. Some relief of the clinical signs was afforded by treatment with diazepam. Marked creatinuria and low serum creatine phosphokinase activity were the only consistent, abnormal laboratory findings. Histologic and cytochemical examinations of muscle biopsy specimens revealed a relative predominance of type I and a deficiency of type II skeletal muscle fibers. Electromyographic studies of the affected dogs indicated a myotonic defect.", "contents": "A muscle disorder of Labrador retrievers characterized by deficiency of type II muscle fibers. A muscle disorder was detected in 5 Labrador Retrievers. The age of onset was less than 6 months. The clinical signs, which were exaggerated with exercise, cold, or excitement, included abnormal head and neck posture, and a stiff, forced, hopping gait. A marked deficiency of skeletal muscle mass resulted in poor conformation. Both black and yellow Labrador Retrievers were affected, and pedigree studies indicated the disorder may have been inherited as an autosomal recessive trait. Some relief of the clinical signs was afforded by treatment with diazepam. Marked creatinuria and low serum creatine phosphokinase activity were the only consistent, abnormal laboratory findings. Histologic and cytochemical examinations of muscle biopsy specimens revealed a relative predominance of type I and a deficiency of type II skeletal muscle fibers. Electromyographic studies of the affected dogs indicated a myotonic defect.", "PMID": 977449}
{"id": "pubmed23n0032_11106", "title": "Intravenously administered ketamine HCl and diazepam for anesthesia of raptors.", "content": "A combination of ketamine HCl and diazepam given intravenously was successfully utilized to induce anesthesia for various surgical procedures in 40 raptors. A dosage of 30 to 40 mg of ketamine HCl/kg of body weight and 1.0 to 1.5 mg of diazepam/kg of body weight was satisfactory for diurnal raptors. Owls were more sensitive to the anesthetic combination, necessitating greater care in anesthetizing them. Abundant body fat was found to be an important factor in calculation of the proper dose of this combination.", "contents": "Intravenously administered ketamine HCl and diazepam for anesthesia of raptors. A combination of ketamine HCl and diazepam given intravenously was successfully utilized to induce anesthesia for various surgical procedures in 40 raptors. A dosage of 30 to 40 mg of ketamine HCl/kg of body weight and 1.0 to 1.5 mg of diazepam/kg of body weight was satisfactory for diurnal raptors. Owls were more sensitive to the anesthetic combination, necessitating greater care in anesthetizing them. Abundant body fat was found to be an important factor in calculation of the proper dose of this combination.", "PMID": 977454}
{"id": "pubmed23n0032_11107", "title": "Intestinal nematodes of white-tailed deer in southeastern United States.", "content": "The small intestine, large intestine, and cecum of 975 white-tailed deer (Odocoileus virginianus) from 83 localities in 13 southeastern states were examined for nematodes. In order of prevalence, the following parasites were found: Capillaria bovis, Eucyathostomum webbi, Monodontus louisianensis, Nematodirus odocoilei, Oesophagostomum venulosum, Trichuris sp, Cooperia punctata, Trichostrongylus longispicularis, Strongyloides sp, Cooperia oncophora, Cooperia spatulata, Cooperia sp, and Trichostrongylus calcaratus. Over one-half of the deer examined were free of intestinal nematodes, and most infected deer harbored few parasites. Of the most prevalent species, C bovis, N odocoilei, O venulosum, and Trichuris sp were widely distributed, whereas M louisianensis and E webbi were restricted in distribution. Severe pathogenicity was associated with only heavy Strongyloides sp infections in 2 penned deer. The findings of this study suggested that white-tailed deer of the Southeast are insignificant in the epizootiology of important intestinal nematodes of domestic livestock.", "contents": "Intestinal nematodes of white-tailed deer in southeastern United States. The small intestine, large intestine, and cecum of 975 white-tailed deer (Odocoileus virginianus) from 83 localities in 13 southeastern states were examined for nematodes. In order of prevalence, the following parasites were found: Capillaria bovis, Eucyathostomum webbi, Monodontus louisianensis, Nematodirus odocoilei, Oesophagostomum venulosum, Trichuris sp, Cooperia punctata, Trichostrongylus longispicularis, Strongyloides sp, Cooperia oncophora, Cooperia spatulata, Cooperia sp, and Trichostrongylus calcaratus. Over one-half of the deer examined were free of intestinal nematodes, and most infected deer harbored few parasites. Of the most prevalent species, C bovis, N odocoilei, O venulosum, and Trichuris sp were widely distributed, whereas M louisianensis and E webbi were restricted in distribution. Severe pathogenicity was associated with only heavy Strongyloides sp infections in 2 penned deer. The findings of this study suggested that white-tailed deer of the Southeast are insignificant in the epizootiology of important intestinal nematodes of domestic livestock.", "PMID": 977456}
{"id": "pubmed23n0032_11108", "title": "Prevalence of Toxoplasma gondii antibodies in wild and domestic animals in northern California.", "content": "Wild and domestic animals from 3 geographic-climatologic areas in northern California were tested for antibodies against Toxoplasma gondii. A total of 2,796 serum samples representing 37 species of wild mammals, 35 species of wild birds, and 5 species of domestic animals were tested by the indirect hemagglutination test. Of 1,174 wild mammal serums tested, 10.8% were positive, which compared with 14.7% of the 1,221 domestic mammal serums. Of 229 wild carnivores tested, 45% were seropositive, including 69% of 86 bobcats, 28% of 58 coyotes, 48% of 25 raccoons, 27% of 26 gray foxes, 22% of 32 striped skunks, a civet cat, and a mink. Serologic evidence of infection was found in 38% of 47 rural domestic cats, but none of the 7 dogs tested was seropositive. Of 160 murid rodents (rats and house mice) in rural habitats, 4% were seropositive, which compared with 2% of 399 cricetine rodents (mostly deer mice) collected from wilderness habitats. Seven percent of 56 wild Artiodactyla (deer and feral pigs) were seropositive, which compared with 15% of 1,048 domestic sheep tested. Of 401 birds tested, 3.5% had antibodies against T gondii. The highest prevalence of antibodies among birds was in crows (14%). Toxoplasma was isolated from 1 raven, by mouse inoculation. In general, the highest prevalence of seropositive carnivores, rodents, and sheep was in the coastal region below 100 ft elevation, where the weather is cool and damp for much of the year. In the central valley the highest prevalence among sheep was in areas under irrigation. The prevalence of antibodies was lowest in the mountain areas, where climatologic extremes prevail at various seasons of the year.", "contents": "Prevalence of Toxoplasma gondii antibodies in wild and domestic animals in northern California. Wild and domestic animals from 3 geographic-climatologic areas in northern California were tested for antibodies against Toxoplasma gondii. A total of 2,796 serum samples representing 37 species of wild mammals, 35 species of wild birds, and 5 species of domestic animals were tested by the indirect hemagglutination test. Of 1,174 wild mammal serums tested, 10.8% were positive, which compared with 14.7% of the 1,221 domestic mammal serums. Of 229 wild carnivores tested, 45% were seropositive, including 69% of 86 bobcats, 28% of 58 coyotes, 48% of 25 raccoons, 27% of 26 gray foxes, 22% of 32 striped skunks, a civet cat, and a mink. Serologic evidence of infection was found in 38% of 47 rural domestic cats, but none of the 7 dogs tested was seropositive. Of 160 murid rodents (rats and house mice) in rural habitats, 4% were seropositive, which compared with 2% of 399 cricetine rodents (mostly deer mice) collected from wilderness habitats. Seven percent of 56 wild Artiodactyla (deer and feral pigs) were seropositive, which compared with 15% of 1,048 domestic sheep tested. Of 401 birds tested, 3.5% had antibodies against T gondii. The highest prevalence of antibodies among birds was in crows (14%). Toxoplasma was isolated from 1 raven, by mouse inoculation. In general, the highest prevalence of seropositive carnivores, rodents, and sheep was in the coastal region below 100 ft elevation, where the weather is cool and damp for much of the year. In the central valley the highest prevalence among sheep was in areas under irrigation. The prevalence of antibodies was lowest in the mountain areas, where climatologic extremes prevail at various seasons of the year.", "PMID": 977457}
{"id": "pubmed23n0032_11109", "title": "Mycobacterium tuberculosis infection in two East African oryxes.", "content": "Tuberculous lesions were observed at necropsy of 2 East African oryxes (Oryx gazella beisa) at a municipal zoological park in Jackson, Ms. Microscopic examination revealed granulomas containing acid-fast bacilli in the lungs and liver of both animals, as well as in the uterus and mediastinal lymph nodes of 1 animal. Mycobacterium tuberculosis was isolated from the tissues of both oryxes and from fluid aspirated from the mammary gland of 1 oryx; the organism was pathogenic for guinea pigs but not for rabbits.", "contents": "Mycobacterium tuberculosis infection in two East African oryxes. Tuberculous lesions were observed at necropsy of 2 East African oryxes (Oryx gazella beisa) at a municipal zoological park in Jackson, Ms. Microscopic examination revealed granulomas containing acid-fast bacilli in the lungs and liver of both animals, as well as in the uterus and mediastinal lymph nodes of 1 animal. Mycobacterium tuberculosis was isolated from the tissues of both oryxes and from fluid aspirated from the mammary gland of 1 oryx; the organism was pathogenic for guinea pigs but not for rabbits.", "PMID": 977460}
{"id": "pubmed23n0032_11110", "title": "Pulmonary tuberculosis in a sulfur-crested cockatoo.", "content": "An adult, female sulfur-crested cockatoo (Kakatoe sp) was examined because of dyspnea associated with hemoptysis. On radiographic examination there was a large cystic pulmonary mass that, on aspiration, was found to contain thick sanguineous fluid. Ziehl-Neelsen staining of aspirate smears revealed numerous acid-fast organisms. Gross necropsy and microscopic findings suggested a diagnosis of tuberculosis, with primary focus of infection in the pulmonary tissues. Bacteriologic isolation and typing confirmed a diagnosis of tuberculosis and established Mycobacterium avium as the etiologic agent.", "contents": "Pulmonary tuberculosis in a sulfur-crested cockatoo. An adult, female sulfur-crested cockatoo (Kakatoe sp) was examined because of dyspnea associated with hemoptysis. On radiographic examination there was a large cystic pulmonary mass that, on aspiration, was found to contain thick sanguineous fluid. Ziehl-Neelsen staining of aspirate smears revealed numerous acid-fast organisms. Gross necropsy and microscopic findings suggested a diagnosis of tuberculosis, with primary focus of infection in the pulmonary tissues. Bacteriologic isolation and typing confirmed a diagnosis of tuberculosis and established Mycobacterium avium as the etiologic agent.", "PMID": 977461}
{"id": "pubmed23n0032_11111", "title": "Tuberculosis in captive exotic birds.", "content": "Avian tuberculosis was studied clinically and pathologically in 137 affected birds from the National Zoological Park during a 7-year period (1969-1975). Twelve of 22 orders exhibited were affected by the disease, and the highest annual mortality was 4% (in 1975). Antemortem diagnosis of early cases of the disease, based on tuberculin testing, and serologic, hematologic, and radiographic studies, was inconsistent and often not conclusive. Pathologically, the diseases primarily involved digestive organs and spleen. There was a spectrum of lesions consisting of nodules of large foamy histiocytes packed with acid-fast bacilli to giant cell-containing granulomas that were often caseous but not cavitated or calcified. Amyloidosis was seen in approximately 20% of the cases. Mycobacterium avium serotype 1 was isolated from 30 tuberculous birds cultured. There was no sex predilection, and most of the affected birds were adults ranging from 1 to 10 years of age.", "contents": "Tuberculosis in captive exotic birds. Avian tuberculosis was studied clinically and pathologically in 137 affected birds from the National Zoological Park during a 7-year period (1969-1975). Twelve of 22 orders exhibited were affected by the disease, and the highest annual mortality was 4% (in 1975). Antemortem diagnosis of early cases of the disease, based on tuberculin testing, and serologic, hematologic, and radiographic studies, was inconsistent and often not conclusive. Pathologically, the diseases primarily involved digestive organs and spleen. There was a spectrum of lesions consisting of nodules of large foamy histiocytes packed with acid-fast bacilli to giant cell-containing granulomas that were often caseous but not cavitated or calcified. Amyloidosis was seen in approximately 20% of the cases. Mycobacterium avium serotype 1 was isolated from 30 tuberculous birds cultured. There was no sex predilection, and most of the affected birds were adults ranging from 1 to 10 years of age.", "PMID": 977462}
{"id": "pubmed23n0032_11112", "title": "Systemic mycoses in marine mammals.", "content": "Thirty-four cases of systemic mycosis in marine mammals were represented by 9 genera of fungi. All cases were characterized by pulmonary involvement, with miliary spread to abdominal organs. Cases were geographically distributed among Hawaii, northern United States, Europe, New Zealand, and southern Asia.", "contents": "Systemic mycoses in marine mammals. Thirty-four cases of systemic mycosis in marine mammals were represented by 9 genera of fungi. All cases were characterized by pulmonary involvement, with miliary spread to abdominal organs. Cases were geographically distributed among Hawaii, northern United States, Europe, New Zealand, and southern Asia.", "PMID": 977467}
{"id": "pubmed23n0032_11113", "title": "Dermatophilosis among wild raccoons in New York State.", "content": "Dermatophilus congolensis was established as the cause of dermatitis in 8 raccoons, as determined from examination of carcasses and untreated hides. Encrusted lesions were restricted to or more pronounced in the areas around the eyes, along the snout, and on the skin in the tarsal and carpal regions. The causative agent was demonstrated through direct examination of stained paraffin sections, isolation in pure culture, or examination of stained smears from lesions induced experimentally in rabbits with material from the affected raccoons.", "contents": "Dermatophilosis among wild raccoons in New York State. Dermatophilus congolensis was established as the cause of dermatitis in 8 raccoons, as determined from examination of carcasses and untreated hides. Encrusted lesions were restricted to or more pronounced in the areas around the eyes, along the snout, and on the skin in the tarsal and carpal regions. The causative agent was demonstrated through direct examination of stained paraffin sections, isolation in pure culture, or examination of stained smears from lesions induced experimentally in rabbits with material from the affected raccoons.", "PMID": 977468}
{"id": "pubmed23n0032_11114", "title": "Myopathy and myoglobinuria in a wild white-tailed deer.", "content": "Fatal myopathy similar to \"capture myopathy\" described for African game was diagnosed in a wild white-tailed deer. Clinical signs included depression, inability to rise or stand, and myoglobinuria. Values for serum creatine phosphokinase, glutamic oxaloacetic transaminase, and blood urea nitrogen were high. The deer died 42 hours after capture. At necropsy the muscles of the limbs had a waxy, \"cooked\" appearance and the kidneys were brown. Microscopic findings included severe degeneration and fragmentation of skeletal muscle fibers, nephrosis, centrilobular hepatic necrosis, myocardial degeneration, and anoxic neuronal degeneration.", "contents": "Myopathy and myoglobinuria in a wild white-tailed deer. Fatal myopathy similar to \"capture myopathy\" described for African game was diagnosed in a wild white-tailed deer. Clinical signs included depression, inability to rise or stand, and myoglobinuria. Values for serum creatine phosphokinase, glutamic oxaloacetic transaminase, and blood urea nitrogen were high. The deer died 42 hours after capture. At necropsy the muscles of the limbs had a waxy, \"cooked\" appearance and the kidneys were brown. Microscopic findings included severe degeneration and fragmentation of skeletal muscle fibers, nephrosis, centrilobular hepatic necrosis, myocardial degeneration, and anoxic neuronal degeneration.", "PMID": 977470}
{"id": "pubmed23n0032_11115", "title": "Fluorescein angiography of the normal and diseased ocular fundi of the laboratory dog.", "content": "A method was determined for fluorescein angiography of the ocular fundus of the laboratory Beagle, using a portable fluorescein fundus camera. A 10% solution of sodium fluorescein was injected by a syringe pump in the right cephalic vein of each dog. Acepromazine maleate (0.5 mg/kg, IV) with ketamine hydrochloride (10 mg/kg, IM) provided chemical restraint. Of the 10-, 25-, and 50-mg/kg dosages of fluorescein, 25 mg/kg permitted satisfactory fluorescence of the choroidal and retinal vasculature and the recirculation phase. High-speed color film permitted good quality photography of the tapetal and nontapetal retinal vessels. Reduced retinal blood vessel and pigment epithelium permeability was demonstrated by fluorescein angiography in dogs with chorioretinitis associated with systemic blastomycosis, retinitis associated with distemper, serous retinal detachment, and preretinal hemorrhage.", "contents": "Fluorescein angiography of the normal and diseased ocular fundi of the laboratory dog. A method was determined for fluorescein angiography of the ocular fundus of the laboratory Beagle, using a portable fluorescein fundus camera. A 10% solution of sodium fluorescein was injected by a syringe pump in the right cephalic vein of each dog. Acepromazine maleate (0.5 mg/kg, IV) with ketamine hydrochloride (10 mg/kg, IM) provided chemical restraint. Of the 10-, 25-, and 50-mg/kg dosages of fluorescein, 25 mg/kg permitted satisfactory fluorescence of the choroidal and retinal vasculature and the recirculation phase. High-speed color film permitted good quality photography of the tapetal and nontapetal retinal vessels. Reduced retinal blood vessel and pigment epithelium permeability was demonstrated by fluorescein angiography in dogs with chorioretinitis associated with systemic blastomycosis, retinitis associated with distemper, serous retinal detachment, and preretinal hemorrhage.", "PMID": 977471}
{"id": "pubmed23n0032_11116", "title": "Immune response of channel catfish under different environmental conditions.", "content": "Channel catfish were maintained under conditions of low (15.4 C) and fluctuating (15.4 to 26.9 C/24 hours) temperatures, low (126 mg/L NO3-N) and high (289 mg/L NO3-N) nitrate in recirculating systems, crowding (171 g body mass/L), and fasting. They were vaccinated with formalin-killed enteric red-mouth bacterium, and antibody titers were monitored weekly for 10 weeks. Only those fish maintained in low or fluctuating temperature environments had significant (P less than 0.01) immunosuppression. The other environmental conditions studies, which are commonly encountered in intensive fish culture operations, did not compromise the humoral immune response of channel catfish.", "contents": "Immune response of channel catfish under different environmental conditions. Channel catfish were maintained under conditions of low (15.4 C) and fluctuating (15.4 to 26.9 C/24 hours) temperatures, low (126 mg/L NO3-N) and high (289 mg/L NO3-N) nitrate in recirculating systems, crowding (171 g body mass/L), and fasting. They were vaccinated with formalin-killed enteric red-mouth bacterium, and antibody titers were monitored weekly for 10 weeks. Only those fish maintained in low or fluctuating temperature environments had significant (P less than 0.01) immunosuppression. The other environmental conditions studies, which are commonly encountered in intensive fish culture operations, did not compromise the humoral immune response of channel catfish.", "PMID": 977474}
{"id": "pubmed23n0032_11117", "title": "An ultrastructural survey of cat dentinal tubules.", "content": "The extent of the odontoblast process, the distribution of intratubular nerve-like structures, and tubule dimensions, have been measured in cat dentine. The odontoblast process varies in extent throughout the tooth. At most levels it is limited to inner dentine, extending further out only in the cervical region. This variation supports the view that the limited extent of the process occurs in vivo and is not an artefact of fixation. No nerve-like processes were found within dentinal tubules in this study, indicating the rarity of such structures. The tubules in the dentine of the cat are more variable in number but narrower than those of man and occupy a relatively smaller proportion of the volume of the tissue. Some factor other than tubule numbers and size must be responsible for the greater access which chemical stimuli seem to have to nerve terminals beneath cat, as opposed to human, dentine.", "contents": "An ultrastructural survey of cat dentinal tubules. The extent of the odontoblast process, the distribution of intratubular nerve-like structures, and tubule dimensions, have been measured in cat dentine. The odontoblast process varies in extent throughout the tooth. At most levels it is limited to inner dentine, extending further out only in the cervical region. This variation supports the view that the limited extent of the process occurs in vivo and is not an artefact of fixation. No nerve-like processes were found within dentinal tubules in this study, indicating the rarity of such structures. The tubules in the dentine of the cat are more variable in number but narrower than those of man and occupy a relatively smaller proportion of the volume of the tissue. Some factor other than tubule numbers and size must be responsible for the greater access which chemical stimuli seem to have to nerve terminals beneath cat, as opposed to human, dentine.", "PMID": 977475}
{"id": "pubmed23n0032_11118", "title": "Compensatory muscular hypertrophy in the extensor digitorum longus muscle of the mouse.", "content": "Hypertrophy of the extensor digitorum longus muscle after surgical removal of its companion tibialis anterior muscle has been studied in mice. Early, intermediate and late stages in the process of hypertrophy have been defined. The proportional increase in the man cross sectional area of fibres in a hypertrophic muscle consistently exceeded the proportional increase in the weight of the same muscle in all animals. This suggests that hypoplasis (loss of fibres) was occurring. This work was carried out with the aid of grants from the Medical Research Council and Science Research Council of Great Britain.", "contents": "Compensatory muscular hypertrophy in the extensor digitorum longus muscle of the mouse. Hypertrophy of the extensor digitorum longus muscle after surgical removal of its companion tibialis anterior muscle has been studied in mice. Early, intermediate and late stages in the process of hypertrophy have been defined. The proportional increase in the man cross sectional area of fibres in a hypertrophic muscle consistently exceeded the proportional increase in the weight of the same muscle in all animals. This suggests that hypoplasis (loss of fibres) was occurring. This work was carried out with the aid of grants from the Medical Research Council and Science Research Council of Great Britain.", "PMID": 977476}
{"id": "pubmed23n0032_11119", "title": "Musculature of facial scent glands in the muntjac.", "content": "The muscles associated with the very large preorbital glands of muntjacs (Muntiacus) are described. Although the two muntjac fawns examined were only 10 days old, their muscles were proportionately larger than those in adult specimens of North American cervids. A muscle, not found in North American cervids, but well developed in muntjacs, is probably responsible for the eversion of muntjacs' preorbital glands.", "contents": "Musculature of facial scent glands in the muntjac. The muscles associated with the very large preorbital glands of muntjacs (Muntiacus) are described. Although the two muntjac fawns examined were only 10 days old, their muscles were proportionately larger than those in adult specimens of North American cervids. A muscle, not found in North American cervids, but well developed in muntjacs, is probably responsible for the eversion of muntjacs' preorbital glands.", "PMID": 977477}
{"id": "pubmed23n0032_11120", "title": "The development of the dorsal funiculus in the human spinal cord.", "content": "1. The development of the dorsal funiculus of the human cord is described from the second month to the end of the fourth. 2. The fasciculus cuneatus arises as a distinct bundle of fibres near the mid-line in the ninth week. This gradually spreads laterally, and to it is joined the more medial fasciculus gracilis. 3. The original embryonic dorsal marginal layer is displaced laterally, and may possibly become the dorsolateral tract adjacent to the dorsal horn. 4. The appearance of the future 'epicritic' pathway is accompanied by a marked wave of differentiation in cervical and thoracic ganglia. 5. The implications of these findings are discussed in relation to the problems of peripheral and central factors in nervous development.", "contents": "The development of the dorsal funiculus in the human spinal cord. 1. The development of the dorsal funiculus of the human cord is described from the second month to the end of the fourth. 2. The fasciculus cuneatus arises as a distinct bundle of fibres near the mid-line in the ninth week. This gradually spreads laterally, and to it is joined the more medial fasciculus gracilis. 3. The original embryonic dorsal marginal layer is displaced laterally, and may possibly become the dorsolateral tract adjacent to the dorsal horn. 4. The appearance of the future 'epicritic' pathway is accompanied by a marked wave of differentiation in cervical and thoracic ganglia. 5. The implications of these findings are discussed in relation to the problems of peripheral and central factors in nervous development.", "PMID": 977478}
{"id": "pubmed23n0032_11121", "title": "The ultrastructure of implanted trophoblast cells of the yellow agouti mouse.", "content": "An electron microscope study of ultrathin sections of trophoblast cells of implanting blastocysts resulting from the crossing of heterozygous yellow agouti mice has been conducted. Several conditions for successful preparation of the blastocysts have been described. These include avoidance of the use of sucrose in the buffers, reduction of specimen washing times and exclusion of propylene oxide from the dehydration procedure. Electron micrographs of cells from a blastocyst considered, on the basis of light microscopy, to be 'abnormal' revealed a cytoplasm with many 'empty' areas and numerous 'vacuolated' mitochondria. Electron micrographs of cells from blastocysts considered to be 'normal' revealed, in one instance, a preponderance of cells with very few of these 'abnormal' features, and, in the other instance, a preponderance of cells with many abnormalities. Both 'normal' blastocysts revealed cellular features usually regarded as either primitive or pathological, namely tight junctions, vacuolated mitochondria and mitochondria with cristae oriented parallel to their long axis. It was generally concluded that the appearance of the blastocysts, in the light microscope, at 100-105 hours of development, could not be used as a valid criterion of normality or abnormality, for, although a blastocyst classified as 'abnormal' under the light microscope appeared 'abnormal' also in the electron microscope, those classified as 'normal' under the light microscope appeared either 'normal' or 'abnormal' in the electron microscope. This disturbing situation may indicate that the electron microscope is diagnosing 'abnormality' before the light microscope. The presence of immature forms of organelles (e.g., vacuolated mitochondria) indicates either a failure to mature or regression. Failure to mature could be benign or of sinister import.", "contents": "The ultrastructure of implanted trophoblast cells of the yellow agouti mouse. An electron microscope study of ultrathin sections of trophoblast cells of implanting blastocysts resulting from the crossing of heterozygous yellow agouti mice has been conducted. Several conditions for successful preparation of the blastocysts have been described. These include avoidance of the use of sucrose in the buffers, reduction of specimen washing times and exclusion of propylene oxide from the dehydration procedure. Electron micrographs of cells from a blastocyst considered, on the basis of light microscopy, to be 'abnormal' revealed a cytoplasm with many 'empty' areas and numerous 'vacuolated' mitochondria. Electron micrographs of cells from blastocysts considered to be 'normal' revealed, in one instance, a preponderance of cells with very few of these 'abnormal' features, and, in the other instance, a preponderance of cells with many abnormalities. Both 'normal' blastocysts revealed cellular features usually regarded as either primitive or pathological, namely tight junctions, vacuolated mitochondria and mitochondria with cristae oriented parallel to their long axis. It was generally concluded that the appearance of the blastocysts, in the light microscope, at 100-105 hours of development, could not be used as a valid criterion of normality or abnormality, for, although a blastocyst classified as 'abnormal' under the light microscope appeared 'abnormal' also in the electron microscope, those classified as 'normal' under the light microscope appeared either 'normal' or 'abnormal' in the electron microscope. This disturbing situation may indicate that the electron microscope is diagnosing 'abnormality' before the light microscope. The presence of immature forms of organelles (e.g., vacuolated mitochondria) indicates either a failure to mature or regression. Failure to mature could be benign or of sinister import.", "PMID": 977479}
{"id": "pubmed23n0032_11122", "title": "An ultrastructural study of keratinized epithelia in the rat soft palate.", "content": "The three kinds of keratinized epithelium evident on gross examination of the soft palate of Wistar albino rats were studied by scanning and transmission electron microscopy. Scanning indicated a central area where the epithelium showed numerous fine folds and papillae, more lateral areas which were smooth, and most lateral areas with fine folds only. At high magnifications the cell surfaces were seen to be pitted with numerous small craters. However, all three epithelia had a similar appearance under the transmission electron microscope, namely that of keratinized oral epithelia. The gross surface differences probably reflect functional modifications.", "contents": "An ultrastructural study of keratinized epithelia in the rat soft palate. The three kinds of keratinized epithelium evident on gross examination of the soft palate of Wistar albino rats were studied by scanning and transmission electron microscopy. Scanning indicated a central area where the epithelium showed numerous fine folds and papillae, more lateral areas which were smooth, and most lateral areas with fine folds only. At high magnifications the cell surfaces were seen to be pitted with numerous small craters. However, all three epithelia had a similar appearance under the transmission electron microscope, namely that of keratinized oral epithelia. The gross surface differences probably reflect functional modifications.", "PMID": 977480}
{"id": "pubmed23n0032_11123", "title": "Evaluation of intrahepatic arterial branching patterns in corrosion casts.", "content": "A study of the branching pattern of the hepatic artery in 85 human livers has been undertaken in corrosion casts. The various branching patterns observed are described in detail and compared with observations of other workers.", "contents": "Evaluation of intrahepatic arterial branching patterns in corrosion casts. A study of the branching pattern of the hepatic artery in 85 human livers has been undertaken in corrosion casts. The various branching patterns observed are described in detail and compared with observations of other workers.", "PMID": 977481}
{"id": "pubmed23n0032_11124", "title": "Changes in articular cartilage following intraarticular injection of tritiated glyceryl trioleate.", "content": "The effects of intra-articular injections of non-radioactive and tritium-labelled glyceryl trioleate into the mandibular and knee joints of adult rabbits have been investigated using autoradiographic and histochemical techniques and electron microscopy. As observed at the fourth day after operation, fat droplets accumulate in cells of the fibrous, intermediate and cartilaginous layers of mandibular condylar, and in the superficial and upper middle (rather than the deeper) zones of femoral condylar cartilage. Autoradiography of frozen sections shows that numerous silver grains are located over these fat-laden cells following injection of trioleate which has been labelled in the fatty acid moiety of the molecule. In the knee joint the number of grains is directly related to the amount of lipid in the cell. Following injection of glyceryl-labelled trioleate no such result is obtained; it seems doubtful whether or not there is any uptake of this label. However, synovial membrane from the knee joint appears to take up both kinds of trioleate. Results of histochemical methods of NADH diaphorase, lactic dehydrogenase, acid phosphatase and beta-glucosaminidase are consistent with ultrastructural evidence of degeneration in some chondrocytes and of loss of ground substance from the matrix. A raised level of alpha-glycerophosphate dehydrogenase activity is probably associated with synthesis of endogenous glycerol for re-esterification of absorbed fatty acids, and enhanced activity of UDPglucose dehydrogenase with the chondrocytic reaction to matrix depletion. Apart from the increase in fat content, ultrastructural features in injected knee joints include flattening of cell processes against the chondrocyte surface and more abundant intracytoplasmic filaments. Injected mandibular joints show little evidence of these changes although the number of cells in the cartilage appears to be greatly reduced. No extracellular fat droplets occur in femoral cartilage, but material similar in electron density to intracellular fat is observed at the external aspect of some mandibular chondrocytes. The findings indicate that the fatty acid portion of triglyceride injected intra-articularly is taken up by the chondrocytes and retained until at least the fourth day after injection. It is suggested that prior lipolysis takes place either in the synovial cavity (or membrane) or at the chondrocyte surface, but it is uncertain how or in what form fat traverses the matrix. Lipoarthrosis appears to produce changes in the chondrocytes which are thought to be pathological; a number of cell deaths occur. The possibility that gross degeneration of the articular cartilage may ensue is subject to further investigation.", "contents": "Changes in articular cartilage following intraarticular injection of tritiated glyceryl trioleate. The effects of intra-articular injections of non-radioactive and tritium-labelled glyceryl trioleate into the mandibular and knee joints of adult rabbits have been investigated using autoradiographic and histochemical techniques and electron microscopy. As observed at the fourth day after operation, fat droplets accumulate in cells of the fibrous, intermediate and cartilaginous layers of mandibular condylar, and in the superficial and upper middle (rather than the deeper) zones of femoral condylar cartilage. Autoradiography of frozen sections shows that numerous silver grains are located over these fat-laden cells following injection of trioleate which has been labelled in the fatty acid moiety of the molecule. In the knee joint the number of grains is directly related to the amount of lipid in the cell. Following injection of glyceryl-labelled trioleate no such result is obtained; it seems doubtful whether or not there is any uptake of this label. However, synovial membrane from the knee joint appears to take up both kinds of trioleate. Results of histochemical methods of NADH diaphorase, lactic dehydrogenase, acid phosphatase and beta-glucosaminidase are consistent with ultrastructural evidence of degeneration in some chondrocytes and of loss of ground substance from the matrix. A raised level of alpha-glycerophosphate dehydrogenase activity is probably associated with synthesis of endogenous glycerol for re-esterification of absorbed fatty acids, and enhanced activity of UDPglucose dehydrogenase with the chondrocytic reaction to matrix depletion. Apart from the increase in fat content, ultrastructural features in injected knee joints include flattening of cell processes against the chondrocyte surface and more abundant intracytoplasmic filaments. Injected mandibular joints show little evidence of these changes although the number of cells in the cartilage appears to be greatly reduced. No extracellular fat droplets occur in femoral cartilage, but material similar in electron density to intracellular fat is observed at the external aspect of some mandibular chondrocytes. The findings indicate that the fatty acid portion of triglyceride injected intra-articularly is taken up by the chondrocytes and retained until at least the fourth day after injection. It is suggested that prior lipolysis takes place either in the synovial cavity (or membrane) or at the chondrocyte surface, but it is uncertain how or in what form fat traverses the matrix. Lipoarthrosis appears to produce changes in the chondrocytes which are thought to be pathological; a number of cell deaths occur. The possibility that gross degeneration of the articular cartilage may ensue is subject to further investigation.", "PMID": 977482}
{"id": "pubmed23n0032_11125", "title": "A multiple-baseline analysis of social-skills training in chronic schizophrenics.", "content": "Social-skills training was applied to two male chronic schizophrenics. Component behaviors of social skill requiring modification were identified for each patient by rating videotapes of role-played interactions. Training involved instructions and feedback for one subject and instructions, feedback, and modelling for the second. Target behaviors were treated sequentially and cumulatively in a multiple-baseline format. Training was applied for both positive and negative assertion and for situation involving males and females. The results were positive for all behaviors for both patients. Follow-ups at 2, 4, 6, and 8 weeks after training indicated that most effects were maintained at near-treatment levels.", "contents": "A multiple-baseline analysis of social-skills training in chronic schizophrenics. Social-skills training was applied to two male chronic schizophrenics. Component behaviors of social skill requiring modification were identified for each patient by rating videotapes of role-played interactions. Training involved instructions and feedback for one subject and instructions, feedback, and modelling for the second. Target behaviors were treated sequentially and cumulatively in a multiple-baseline format. Training was applied for both positive and negative assertion and for situation involving males and females. The results were positive for all behaviors for both patients. Follow-ups at 2, 4, 6, and 8 weeks after training indicated that most effects were maintained at near-treatment levels.", "PMID": 977514}
{"id": "pubmed23n0032_11126", "title": "Reducing time limits: a means to increase behavior of retardates.", "content": "A common assumption in special education is that temporal limits for a task should be expanded so that ample time is provided for completing the work. This study describes the opposite strategy of restricting temporal limits to augment academic performance. Three educable retarded children received token reinforcement contingent on the number of correct math problems answered during daily sessions. A reversal design was used to assess the effects of an abrupt reduction in time limits (20-5-20 min) and a graduated sequence of reductions (20-15-10-5-20 min). The graduated sequence resulted in rate increases of correct responding ranging from 125% to 266% and these gains endured when temporal limits were again expanded. In contrast, the abrupt shift produced interfering emotional behaviors and rate decreases in academic performance of 25% to 80%. The findings indicate that systematically restricting temporal limits for an academic task can further enhance the performance of slow learners already maintained by a token system.", "contents": "Reducing time limits: a means to increase behavior of retardates. A common assumption in special education is that temporal limits for a task should be expanded so that ample time is provided for completing the work. This study describes the opposite strategy of restricting temporal limits to augment academic performance. Three educable retarded children received token reinforcement contingent on the number of correct math problems answered during daily sessions. A reversal design was used to assess the effects of an abrupt reduction in time limits (20-5-20 min) and a graduated sequence of reductions (20-15-10-5-20 min). The graduated sequence resulted in rate increases of correct responding ranging from 125% to 266% and these gains endured when temporal limits were again expanded. In contrast, the abrupt shift produced interfering emotional behaviors and rate decreases in academic performance of 25% to 80%. The findings indicate that systematically restricting temporal limits for an academic task can further enhance the performance of slow learners already maintained by a token system.", "PMID": 977515}
{"id": "pubmed23n0032_11127", "title": "The cellblock token economy: token reinforcement procedures in a maximum security correctional institution for adult male felons.", "content": "Two experiments were conducted (1) to explore the application of token reinforcement procedures in a maximum security correctional institution for adult male felons and (2) to determine to what extent the reinforcement procedures disrupted the day-to-day lives of inmate participants. In Experiment 1, an expanded reversal design revealed that the combination of praise and token reinforcement was more effective than the combinations of praise and noncontingent token award or direct commands on four common institutional activities. The latter two combinations were not found to be any more effective than praise alone. Experiment 2, which also employed a reversal design, indicated that the high levels of performance observed during the token reinforcement phases of Experiment 1 could be attained without subjecting participants to undue hardship in the form of increased deprivation of either social intercourse or the opportunity to engage in recreational and entertainment activities. Client safeguards are discussed in detail.", "contents": "The cellblock token economy: token reinforcement procedures in a maximum security correctional institution for adult male felons. Two experiments were conducted (1) to explore the application of token reinforcement procedures in a maximum security correctional institution for adult male felons and (2) to determine to what extent the reinforcement procedures disrupted the day-to-day lives of inmate participants. In Experiment 1, an expanded reversal design revealed that the combination of praise and token reinforcement was more effective than the combinations of praise and noncontingent token award or direct commands on four common institutional activities. The latter two combinations were not found to be any more effective than praise alone. Experiment 2, which also employed a reversal design, indicated that the high levels of performance observed during the token reinforcement phases of Experiment 1 could be attained without subjecting participants to undue hardship in the form of increased deprivation of either social intercourse or the opportunity to engage in recreational and entertainment activities. Client safeguards are discussed in detail.", "PMID": 977516}
{"id": "pubmed23n0032_11128", "title": "Employing task arrangements and verbal contingencies to promote verbalizations between retarded children.", "content": "This study investigated the effects of arranging task events for interdependence, to increase the probability of social responding. During task interdependence, the subjects, participating in dyads and a four-person group, obtained task materials (a puzzle piece) from their partner before completing their task (appropriately placing the puzzle piece). The verbal contingency required a verbal request to precede a subject's receiving a task material from his partner. The verbal contingency yoked with task interdependence made task completion contingent on the appropriate verbalization. The findings from two experiments suggested that task interdependence was sufficient to increase partner-directed verbalizations for three of the four subjects. When the verbal contingency was added, all subjects increased their requests and other verbalizations to partner. Applied to a four-person group, the verbal contingency yoked with varying levels of task interdependence correspondingly affected the pattern and level of group communications. The greater the task interdependence, i.e., the more members each subject depended on to complete his task, the more complex the social network of verbal contacts, and the higher the level of both requests and other verbalizations for the group.", "contents": "Employing task arrangements and verbal contingencies to promote verbalizations between retarded children. This study investigated the effects of arranging task events for interdependence, to increase the probability of social responding. During task interdependence, the subjects, participating in dyads and a four-person group, obtained task materials (a puzzle piece) from their partner before completing their task (appropriately placing the puzzle piece). The verbal contingency required a verbal request to precede a subject's receiving a task material from his partner. The verbal contingency yoked with task interdependence made task completion contingent on the appropriate verbalization. The findings from two experiments suggested that task interdependence was sufficient to increase partner-directed verbalizations for three of the four subjects. When the verbal contingency was added, all subjects increased their requests and other verbalizations to partner. Applied to a four-person group, the verbal contingency yoked with varying levels of task interdependence correspondingly affected the pattern and level of group communications. The greater the task interdependence, i.e., the more members each subject depended on to complete his task, the more complex the social network of verbal contacts, and the higher the level of both requests and other verbalizations for the group.", "PMID": 977517}
{"id": "pubmed23n0032_11129", "title": "The development and functional control of reading-comprehension behavior.", "content": "Reading comprehension, indicated by motor behavior and multiple-choice picture selection called for in written instructions, was taught to an autistic child using verbal prompts, modelling, and physical guidance. The child was rewarded for correct behaviors to training items; nonrewarded probes were used to assess generalization. Probable maintaining events were assessed through their sequential removal in a reversal design. Results showed: (a) following acquisition, performance was maintained at a near-100% level when candy, praise, attention, and training were removed, (b) absence of other persons was correlated with a marked decrease in performance, whereas their presence was associated with performance at near 100%, and (c) performance generalized to probes and across experimenters. Rewards, which may have been reinforcing during acquisition, did not appear necessary to maintain later performance. Instead, presence of others (a setting event) was demonstrated to have control over maintained performance.", "contents": "The development and functional control of reading-comprehension behavior. Reading comprehension, indicated by motor behavior and multiple-choice picture selection called for in written instructions, was taught to an autistic child using verbal prompts, modelling, and physical guidance. The child was rewarded for correct behaviors to training items; nonrewarded probes were used to assess generalization. Probable maintaining events were assessed through their sequential removal in a reversal design. Results showed: (a) following acquisition, performance was maintained at a near-100% level when candy, praise, attention, and training were removed, (b) absence of other persons was correlated with a marked decrease in performance, whereas their presence was associated with performance at near 100%, and (c) performance generalized to probes and across experimenters. Rewards, which may have been reinforcing during acquisition, did not appear necessary to maintain later performance. Instead, presence of others (a setting event) was demonstrated to have control over maintained performance.", "PMID": 977518}
{"id": "pubmed23n0032_11130", "title": "The generalization effects of parent training across stimulus settings.", "content": "Five parents of nonverbal children were trained in two home settings to modify antecedents and consequences to their children's vocalizations. Generalization effects of the parent training on both the parent's and children's behaviors under different stimulus conditions were investigated using multiple-baseline designs. Increases in parent prompting and reinforcing their children's vocalizations generalized only minimally to a new setting in the home where parent training had not occurred. Child increases in vocalizations produced by the parents in the training settings did generalize to this new setting in the home. There was minimal generalization of child vocalizations to a free-play setting at school. In a formal speech session conducted by a behavior specialist at school, only one child showed definite increases in acquisition rate as a function of the parents starting to train the sound at home.", "contents": "The generalization effects of parent training across stimulus settings. Five parents of nonverbal children were trained in two home settings to modify antecedents and consequences to their children's vocalizations. Generalization effects of the parent training on both the parent's and children's behaviors under different stimulus conditions were investigated using multiple-baseline designs. Increases in parent prompting and reinforcing their children's vocalizations generalized only minimally to a new setting in the home where parent training had not occurred. Child increases in vocalizations produced by the parents in the training settings did generalize to this new setting in the home. There was minimal generalization of child vocalizations to a free-play setting at school. In a formal speech session conducted by a behavior specialist at school, only one child showed definite increases in acquisition rate as a function of the parents starting to train the sound at home.", "PMID": 977519}
{"id": "pubmed23n0032_11131", "title": "Comparison of an electronic spirometer with a pediatric Wright Peak Flow Meter and their relationship to clinical symptomatology in asthmatic children.", "content": "To evaluate the correlations between the Wright Peak Flow Meter and an Electronic Spirometer with clinical symptomatology in chronic asthmatic chidlren, objective measurements of Peak Flow (PF) on both electronic (Vanguard Electric Spirometer) and mechanical (Wright Peak Flow Meter) Spirometers, Forced Vital Capacity (FVC), Forced, Expiratory Volune 1/2 second (FEV 0.5), Forced Expiratory Volume 1/2 per cent (FEV 0.5%), AND Maximal Midexpiratory Flow Rate (MMEFR) were obtained. Subjective observations of clinical wheezing were recorded immediately before each flow rate by trained nurses. PF determinations of both the mechanical and electronic spirometers were in close agreement. PF on the electronic spirometer correlated best with clinical wheezing in this study. The MMEFR calculated from the electronically produced graphys had the nex best coefficient of corelation. These were followed by the PF measured mechanically on the WPFM.", "contents": "Comparison of an electronic spirometer with a pediatric Wright Peak Flow Meter and their relationship to clinical symptomatology in asthmatic children. To evaluate the correlations between the Wright Peak Flow Meter and an Electronic Spirometer with clinical symptomatology in chronic asthmatic chidlren, objective measurements of Peak Flow (PF) on both electronic (Vanguard Electric Spirometer) and mechanical (Wright Peak Flow Meter) Spirometers, Forced Vital Capacity (FVC), Forced, Expiratory Volune 1/2 second (FEV 0.5), Forced Expiratory Volume 1/2 per cent (FEV 0.5%), AND Maximal Midexpiratory Flow Rate (MMEFR) were obtained. Subjective observations of clinical wheezing were recorded immediately before each flow rate by trained nurses. PF determinations of both the mechanical and electronic spirometers were in close agreement. PF on the electronic spirometer correlated best with clinical wheezing in this study. The MMEFR calculated from the electronically produced graphys had the nex best coefficient of corelation. These were followed by the PF measured mechanically on the WPFM.", "PMID": 977532}
{"id": "pubmed23n0032_11132", "title": "Release of enzymes by normal and wall-free cells of Chlamydomonas.", "content": "The phosphatase produced by the wild-type strain of Chlamydomonas reinhardi in media deprived of inorganic phosphate are found partly inside and partly outside the cells. The same enzymes are almost completely released by a mutant strain defective in cell wall formation. It is proposed that the failure of cell wall mutants to survive in certain conditions is related to their inability to retain certain essential compounds that are normally associated to the cell wall.", "contents": "Release of enzymes by normal and wall-free cells of Chlamydomonas. The phosphatase produced by the wild-type strain of Chlamydomonas reinhardi in media deprived of inorganic phosphate are found partly inside and partly outside the cells. The same enzymes are almost completely released by a mutant strain defective in cell wall formation. It is proposed that the failure of cell wall mutants to survive in certain conditions is related to their inability to retain certain essential compounds that are normally associated to the cell wall.", "PMID": 977535}
{"id": "pubmed23n0032_11133", "title": "Selective inactivation of nitrogenase in Azotobacter vinelandii batch cultures.", "content": "When the exhaustion of sucrose or sulfate or the induction of encystment (by incubation in 0.2% beta-hydroxybutyrate) leads to termination of growth in Azotobacter vinelandii batch cultures, the nitrogenase levels in the organisms decreased rapidly, whereas glutamate synthase and glutamine synthetase levels remained unaltered. Glutamate dehydrogenase activities were low during the whole culture cycle, indicating that ammonia assimilation proceeds via glutamine. Toward depletion of sucrose or during induction of encystment, slight secretion of ammonia with subsequent reabsorption was occasionally observed, whereas massive ammonia excretion occurred when the sulfate became exhausted. The extracellular ammonia levels were paralleled by changes in the glutamine synthetase activity. The inactivation of the nitrogenase is explained as a result of rising oxygen tension, a consequence of a metabolic shift-down (reduced respiration) that occurs in organisms entering the stationary phase.", "contents": "Selective inactivation of nitrogenase in Azotobacter vinelandii batch cultures. When the exhaustion of sucrose or sulfate or the induction of encystment (by incubation in 0.2% beta-hydroxybutyrate) leads to termination of growth in Azotobacter vinelandii batch cultures, the nitrogenase levels in the organisms decreased rapidly, whereas glutamate synthase and glutamine synthetase levels remained unaltered. Glutamate dehydrogenase activities were low during the whole culture cycle, indicating that ammonia assimilation proceeds via glutamine. Toward depletion of sucrose or during induction of encystment, slight secretion of ammonia with subsequent reabsorption was occasionally observed, whereas massive ammonia excretion occurred when the sulfate became exhausted. The extracellular ammonia levels were paralleled by changes in the glutamine synthetase activity. The inactivation of the nitrogenase is explained as a result of rising oxygen tension, a consequence of a metabolic shift-down (reduced respiration) that occurs in organisms entering the stationary phase.", "PMID": 977536}
{"id": "pubmed23n0032_11134", "title": "Teichoic acids and lipids associated with the membrane of a Bacillus licheniformis mutant and the membrane lipids of the parental strain.", "content": "Bacillus licheniformis 6346 MH-1 and a phosphoglucomutase-deficient poorly lytic mutant, B. licheniformis 6346 MH-5, both contain cardiolipin, phosphatidyl ethanolamine, and phosphatidyl glycerol but are devoid of phosphoglycolipids. Gentiobiosyl diglyceride is present in the parent organism but glycolipids are absent from the mutant. Lipoteichoic acid was extracted from the whole cells of MH-5 with hot aqueous phenol and contained fatty acids, glucosamine, and 1,3-polyglycerol phosphate. The fatty acids were predominantly of the branched-chain type and were esterified to hydroxyl groups of a terminal glycerol residue. The polyglycerol phosphate chains contained, on average, 32 to 40 glycerol residues, some of which were substituted at the secondary hydroxyl group with alpha-N-acylglucosaminyl residues. Phenol extraction of the supernatant fluid that remained when walls were removed from preparations of disrupted cells of MH-5 yielded membrane teichoic acid, which consisted of substituted polyglycerol phosphate but was devoid of fatty acids.", "contents": "Teichoic acids and lipids associated with the membrane of a Bacillus licheniformis mutant and the membrane lipids of the parental strain. Bacillus licheniformis 6346 MH-1 and a phosphoglucomutase-deficient poorly lytic mutant, B. licheniformis 6346 MH-5, both contain cardiolipin, phosphatidyl ethanolamine, and phosphatidyl glycerol but are devoid of phosphoglycolipids. Gentiobiosyl diglyceride is present in the parent organism but glycolipids are absent from the mutant. Lipoteichoic acid was extracted from the whole cells of MH-5 with hot aqueous phenol and contained fatty acids, glucosamine, and 1,3-polyglycerol phosphate. The fatty acids were predominantly of the branched-chain type and were esterified to hydroxyl groups of a terminal glycerol residue. The polyglycerol phosphate chains contained, on average, 32 to 40 glycerol residues, some of which were substituted at the secondary hydroxyl group with alpha-N-acylglucosaminyl residues. Phenol extraction of the supernatant fluid that remained when walls were removed from preparations of disrupted cells of MH-5 yielded membrane teichoic acid, which consisted of substituted polyglycerol phosphate but was devoid of fatty acids.", "PMID": 977537}
{"id": "pubmed23n0032_11135", "title": "Biosynthesis of saturated and unsaturated fatty acids by a T-strain mycoplasma (Ureaplasma).", "content": "A human T mycoplasma (Ureaplasma urealyticum) incorporated radioactivity into its lipids from [1-14C]acetate in the growth medium. Methanolysis of the lipids showed the label to be confined almost entirely to the methyl esters of the fatty acids. About 80% of the label was associated with the methyl esters of the saturated fatty acids, and the rest was found in the unsaturated methyl ester fraction. Gas-liquid chromatography of the saturated methyl esters showed the label to be present in the peaks of palmitate, myristate, and stearate, whereas in the unsaturated methyl ester fraction most of the radioactivity emerged in the peak of palmitoleate. The addition of either oleic or palmitic acid to the growth medium markedly decreased the organisms' incorporation of radioactivity from acetate. It is concluded that the T mycoplasma strain is capable of de novo synthesis of both saturated and unsaturated fatty acids, in this respect differing from all of the Mycoplasma and Acholeplasma strains investigated to date.", "contents": "Biosynthesis of saturated and unsaturated fatty acids by a T-strain mycoplasma (Ureaplasma). A human T mycoplasma (Ureaplasma urealyticum) incorporated radioactivity into its lipids from [1-14C]acetate in the growth medium. Methanolysis of the lipids showed the label to be confined almost entirely to the methyl esters of the fatty acids. About 80% of the label was associated with the methyl esters of the saturated fatty acids, and the rest was found in the unsaturated methyl ester fraction. Gas-liquid chromatography of the saturated methyl esters showed the label to be present in the peaks of palmitate, myristate, and stearate, whereas in the unsaturated methyl ester fraction most of the radioactivity emerged in the peak of palmitoleate. The addition of either oleic or palmitic acid to the growth medium markedly decreased the organisms' incorporation of radioactivity from acetate. It is concluded that the T mycoplasma strain is capable of de novo synthesis of both saturated and unsaturated fatty acids, in this respect differing from all of the Mycoplasma and Acholeplasma strains investigated to date.", "PMID": 977538}
{"id": "pubmed23n0032_11136", "title": "Isolation and characterization of a glycerol auxotroph of Rhodopseudomonas capsulata: effect of lipid synthesis on the synthesis of photosynthetic pigments.", "content": "A glycerol auxotroph was isolated from Rhodopseudomonas capsulata for use as a system for studying membrane synthesis and function. When the mutant was deprived of glycerol, net phospholipid synthesis ceased immediately and a small amount of free fatty acids accumulated. A turnover of lipid occurred in both deprived and supplemented cultures. Deoxyribonucleic acid and protein synthesis continued for one doubling of cell massand then slowed down in deprived cells. Net ribonucleic acid synthesis slowed down more dramatically. Oxidative phosphorylation activity of membrane preparations from aerobically and semi-anaerobically grown cells appeared unaffected by glycerol deprivation, indicating that simultaneous lipid synthesis is not a requirement for new oxidative phosphorylating activity. In the absence of net phospholipid synthesis, bacteriochlorophyll and carotenoid syntheses were reduced to 30% of the activity of supplemented cultures. Delta-Aminolevulinic acid synthase, the first enzyme on the bacteriochlorophyll pathway that is subject to regulatory control, increased in activity in deprived cultures. Lascelles and Szilagyi (1965) showed an association between phospholipid synthesis and pigment production. They found an increased lipid content associated with pigmented cells. The present results indicate that not only is there an association between lipid and pigment synthesis, but also there is actually a dependence of bacteriochlorophyll synthesis on phospholipid synthesis.", "contents": "Isolation and characterization of a glycerol auxotroph of Rhodopseudomonas capsulata: effect of lipid synthesis on the synthesis of photosynthetic pigments. A glycerol auxotroph was isolated from Rhodopseudomonas capsulata for use as a system for studying membrane synthesis and function. When the mutant was deprived of glycerol, net phospholipid synthesis ceased immediately and a small amount of free fatty acids accumulated. A turnover of lipid occurred in both deprived and supplemented cultures. Deoxyribonucleic acid and protein synthesis continued for one doubling of cell massand then slowed down in deprived cells. Net ribonucleic acid synthesis slowed down more dramatically. Oxidative phosphorylation activity of membrane preparations from aerobically and semi-anaerobically grown cells appeared unaffected by glycerol deprivation, indicating that simultaneous lipid synthesis is not a requirement for new oxidative phosphorylating activity. In the absence of net phospholipid synthesis, bacteriochlorophyll and carotenoid syntheses were reduced to 30% of the activity of supplemented cultures. Delta-Aminolevulinic acid synthase, the first enzyme on the bacteriochlorophyll pathway that is subject to regulatory control, increased in activity in deprived cultures. Lascelles and Szilagyi (1965) showed an association between phospholipid synthesis and pigment production. They found an increased lipid content associated with pigmented cells. The present results indicate that not only is there an association between lipid and pigment synthesis, but also there is actually a dependence of bacteriochlorophyll synthesis on phospholipid synthesis.", "PMID": 977539}
{"id": "pubmed23n0032_11137", "title": "Kinetics and catalytic properties of coenzyme A transferase from Peptostreptococcus elsdenii.", "content": "Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,000). Heat treatment of the crude cell extract to 58 degrees C causes proteolysis of the native enzyme and yields a catalytically active enzyme with an approximate molecular weight of 120,000. The native CoA transferase is specific for CoA esters of short-chain alkyl monocarboxylic acids. With acetate as CoA acceptor the enzyme is active with propionyl-, butyryl-, isobutyryl-, valeryl-, isovaleryl,- and hexanoyl-CoA but not with heptanoyl or longer-chain CoA esters. There is no activity with acetoacetyl-CoA or the CoA esters of dicarboxylic acids. Steady-state kinetics indicated that the reaction proceeds via a classical bi-, bi-ping-pong mechanism. Maximal activity is obtained with propionyl- or butyryl-CoA, and both the Vmax and Km decrease as the alkyl chain length of the CoA ester increases. All CoA esters apompetitive inhibitor although it is not active as a substrate. Evidence for an enzyme CoA intermediate was provided by demonstration of an exchange between 14C-free acids (acetate and butyrate) and their corresponding CoA esters and by isolation of a 3H-labeled CoA enzyme after incubation of the enzyme with 3H-labeled acetyl-CoA. Approximately 2 mol of CoA was bound per mol of enzyme.", "contents": "Kinetics and catalytic properties of coenzyme A transferase from Peptostreptococcus elsdenii. Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,000). Heat treatment of the crude cell extract to 58 degrees C causes proteolysis of the native enzyme and yields a catalytically active enzyme with an approximate molecular weight of 120,000. The native CoA transferase is specific for CoA esters of short-chain alkyl monocarboxylic acids. With acetate as CoA acceptor the enzyme is active with propionyl-, butyryl-, isobutyryl-, valeryl-, isovaleryl,- and hexanoyl-CoA but not with heptanoyl or longer-chain CoA esters. There is no activity with acetoacetyl-CoA or the CoA esters of dicarboxylic acids. Steady-state kinetics indicated that the reaction proceeds via a classical bi-, bi-ping-pong mechanism. Maximal activity is obtained with propionyl- or butyryl-CoA, and both the Vmax and Km decrease as the alkyl chain length of the CoA ester increases. All CoA esters apompetitive inhibitor although it is not active as a substrate. Evidence for an enzyme CoA intermediate was provided by demonstration of an exchange between 14C-free acids (acetate and butyrate) and their corresponding CoA esters and by isolation of a 3H-labeled CoA enzyme after incubation of the enzyme with 3H-labeled acetyl-CoA. Approximately 2 mol of CoA was bound per mol of enzyme.", "PMID": 977540}
{"id": "pubmed23n0032_11138", "title": "Requirement of a cell division step for stalk formation in Caulobacter crescentus.", "content": "Penicillin G at low concentrations blocked cell division in Caulobacter crescentus without inhibiting cell growth. The long filamentous cells formed after two to three generations under these conditions had a stalk at one pole and usually one or more flagella at the opposite pole. The failure of the filaments to form a second stalk at the flagellated pole indicates that stalk formation was dependent upon completion of a step that was also required for cell division. Two observations support this conclusion. (i) Penicillin did not stop the normal development of synchronous swarmer cells into stalked initiation and stalk elongation. (ii) When the action of penicillin was reversed by the addition of penicillinase to cultures of filaments, stalks were not formed at the nonstalked pole until after cell division had occurred; thus the normal order of development events was maintained: cell division leads to stalk formation. These results are consistent with a model in which the organization of the developmental program for stalk formation occurs before cell division as a consequence of steps that branch from the cell division pathway.", "contents": "Requirement of a cell division step for stalk formation in Caulobacter crescentus. Penicillin G at low concentrations blocked cell division in Caulobacter crescentus without inhibiting cell growth. The long filamentous cells formed after two to three generations under these conditions had a stalk at one pole and usually one or more flagella at the opposite pole. The failure of the filaments to form a second stalk at the flagellated pole indicates that stalk formation was dependent upon completion of a step that was also required for cell division. Two observations support this conclusion. (i) Penicillin did not stop the normal development of synchronous swarmer cells into stalked initiation and stalk elongation. (ii) When the action of penicillin was reversed by the addition of penicillinase to cultures of filaments, stalks were not formed at the nonstalked pole until after cell division had occurred; thus the normal order of development events was maintained: cell division leads to stalk formation. These results are consistent with a model in which the organization of the developmental program for stalk formation occurs before cell division as a consequence of steps that branch from the cell division pathway.", "PMID": 977541}
{"id": "pubmed23n0032_11139", "title": "Plasmid deoxyribonucleic acid in Rhizobium trifolii.", "content": "In deoxyribonucleic acid of Rhizobium trifolii centrifuged in cesium chloride-ethidium bromide equilibrium was found a sattelite peak containing covalently closed circular deoxyribonucleic acid. The plasmid had a molecular weight of about 64 x 10(6) shown by sedimentation in sucrose gradients and electron microscopy.", "contents": "Plasmid deoxyribonucleic acid in Rhizobium trifolii. In deoxyribonucleic acid of Rhizobium trifolii centrifuged in cesium chloride-ethidium bromide equilibrium was found a sattelite peak containing covalently closed circular deoxyribonucleic acid. The plasmid had a molecular weight of about 64 x 10(6) shown by sedimentation in sucrose gradients and electron microscopy.", "PMID": 977542}
{"id": "pubmed23n0032_11140", "title": "Occurrence of 3-O-methylmannose in the polysaccharide of Leptospira biflexa Urawa.", "content": "3-O-methylmannose was identified by gas-liquid chromatography-mass spectrometry in the acid hydrolysate of the polysaccharide of Leptospira biflexa Urawa.", "contents": "Occurrence of 3-O-methylmannose in the polysaccharide of Leptospira biflexa Urawa. 3-O-methylmannose was identified by gas-liquid chromatography-mass spectrometry in the acid hydrolysate of the polysaccharide of Leptospira biflexa Urawa.", "PMID": 977543}
{"id": "pubmed23n0032_11141", "title": "Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C.", "content": "We have observed that transcription, involved in production of allophanate hydrolase, is completed 2.5 min after the addition of inducer at 15 degrees C. The rna1 gene product must be functional up unti 10 min; protein synthesis is initiated at 20 min and is terminated by 24 min. Two minutes later, active enzyme appears. The results confirm our earlier observations and eliminate any uncertainty that might have clouded identification of the time within the lag period that is occupied by ribonucleic acid synthesis.", "contents": "Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C. We have observed that transcription, involved in production of allophanate hydrolase, is completed 2.5 min after the addition of inducer at 15 degrees C. The rna1 gene product must be functional up unti 10 min; protein synthesis is initiated at 20 min and is terminated by 24 min. Two minutes later, active enzyme appears. The results confirm our earlier observations and eliminate any uncertainty that might have clouded identification of the time within the lag period that is occupied by ribonucleic acid synthesis.", "PMID": 977544}
{"id": "pubmed23n0032_11142", "title": "Light-induced inhibition of sporulation in Bacillus licheniformis.", "content": "Sporulation of Bacillus licheniformis is inhibited by broad-spectrum light. This phenomenon is intensity dependent and is a near-ultraviolet and blue light effect.", "contents": "Light-induced inhibition of sporulation in Bacillus licheniformis. Sporulation of Bacillus licheniformis is inhibited by broad-spectrum light. This phenomenon is intensity dependent and is a near-ultraviolet and blue light effect.", "PMID": 977545}
{"id": "pubmed23n0032_11143", "title": "Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity.", "content": "A conditional lethal and radiation-sensitive mutant of Schizosaccharomyces pombe is described in which both characteristics result from a single gene mutation. Confirmation of the pleiotropic nature of this mutant was obtained by tetrad analysis and by testing the radiation sensitivity of a large number of revertants that grew normally at the restrictive temperature. The colony-forming ability of the mutant after ultraviolet radiation, gamma radiation, and ethyl methane sulfonate treatment is considerably altered by the post-treatment incubation temperature, showing higher survival at 25 than at 30degreesC. The radiosensitivity of the mutant is also influenced by the stage of growth. The difference in radiation sensitivity between the wild type and mutant is greater when log-phase cultures are compared. The characteristics of this mutant suggest that it is defective in a step common to both deoxyribonucleic acid replication and repair.", "contents": "Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity. A conditional lethal and radiation-sensitive mutant of Schizosaccharomyces pombe is described in which both characteristics result from a single gene mutation. Confirmation of the pleiotropic nature of this mutant was obtained by tetrad analysis and by testing the radiation sensitivity of a large number of revertants that grew normally at the restrictive temperature. The colony-forming ability of the mutant after ultraviolet radiation, gamma radiation, and ethyl methane sulfonate treatment is considerably altered by the post-treatment incubation temperature, showing higher survival at 25 than at 30degreesC. The radiosensitivity of the mutant is also influenced by the stage of growth. The difference in radiation sensitivity between the wild type and mutant is greater when log-phase cultures are compared. The characteristics of this mutant suggest that it is defective in a step common to both deoxyribonucleic acid replication and repair.", "PMID": 977546}
{"id": "pubmed23n0032_11144", "title": "Pentose metabolism in Mycobacterium smegmatis: specificity of induction of pentose isomerases.", "content": "The induction of D-xylose, D-ribose, L-arabinose, and D-lyxose isomerases by various sugars was studied to determine the configuration necessary for induction. D-Xylose isomerase was only induced by D-xylose, whereas D-ribose isomerase was induced by D-ribose, L-rhamnose, and L-lyxose. L-arabinose isomerase was induced by L-arabinose, D-galactose, L-arabitol, D-fucose, and dulcitol, whereas D-lyxose isomerase was induced by D-lyxose, D-mannose, D-ribose, dulcitol, and myoinositol. Some compounds such as dulcitol, D-galactose, and D- or L-fucose which do not support growth are still able to serve as inducers for various pentose isomerases.", "contents": "Pentose metabolism in Mycobacterium smegmatis: specificity of induction of pentose isomerases. The induction of D-xylose, D-ribose, L-arabinose, and D-lyxose isomerases by various sugars was studied to determine the configuration necessary for induction. D-Xylose isomerase was only induced by D-xylose, whereas D-ribose isomerase was induced by D-ribose, L-rhamnose, and L-lyxose. L-arabinose isomerase was induced by L-arabinose, D-galactose, L-arabitol, D-fucose, and dulcitol, whereas D-lyxose isomerase was induced by D-lyxose, D-mannose, D-ribose, dulcitol, and myoinositol. Some compounds such as dulcitol, D-galactose, and D- or L-fucose which do not support growth are still able to serve as inducers for various pentose isomerases.", "PMID": 977547}
{"id": "pubmed23n0032_11145", "title": "Arginine catabolism by Treponema denticola.", "content": "Treponema denticola, an anaerobe commonly present in the human mouth, ferments various amino acids and glucose. Amino acid analyses indicated that substrate amounts of arginine were utilized by T. denticola growing in a complex, serum-containing medium. Cell suspensions metabolized L-arginine to citrulline, NH3, CO2, proline, and small amounts of ornithine. CO2, NH3, ornithine, and proline were produced from L-citrulline by cell suspensions. Determinations of radioactivity in products formed from L-[U-14C]ornithine indicated that cell suspensions converted this amino acid to proline. Furthermore, proline was excreted by cells growing in a complex, arginine-containing medium. Arginine iminohydrolase (deiminase) and ornithine carbamoyltransferase activities were detected in T. denticola cell extracts. Carbamoylphosphate dissimilation by extracts yielded adenosine triphosphate. The data indicate that T. denticola derives energy by dissimilating L-argine via the arginine iminohydrolase pathway. However, unlike some of the other bacteria that utilize this pathway, T. denticola converts to proline much of the ornithine derived from L-arginine.", "contents": "Arginine catabolism by Treponema denticola. Treponema denticola, an anaerobe commonly present in the human mouth, ferments various amino acids and glucose. Amino acid analyses indicated that substrate amounts of arginine were utilized by T. denticola growing in a complex, serum-containing medium. Cell suspensions metabolized L-arginine to citrulline, NH3, CO2, proline, and small amounts of ornithine. CO2, NH3, ornithine, and proline were produced from L-citrulline by cell suspensions. Determinations of radioactivity in products formed from L-[U-14C]ornithine indicated that cell suspensions converted this amino acid to proline. Furthermore, proline was excreted by cells growing in a complex, arginine-containing medium. Arginine iminohydrolase (deiminase) and ornithine carbamoyltransferase activities were detected in T. denticola cell extracts. Carbamoylphosphate dissimilation by extracts yielded adenosine triphosphate. The data indicate that T. denticola derives energy by dissimilating L-argine via the arginine iminohydrolase pathway. However, unlike some of the other bacteria that utilize this pathway, T. denticola converts to proline much of the ornithine derived from L-arginine.", "PMID": 977548}
{"id": "pubmed23n0032_11146", "title": "Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI.", "content": "The bacteriophage Pa16, isolated from soil on Streptomyces albus G, was restricted when transferred from an alternative host back to S. albus G. Extracted unmodified Pa16 deoxyribonucleic acid was cleaved at a single site by a cell-free extract of S. albus G. Fractions cleaving Pal6 deoxyribonucleic acid contained the endonuclease SalI first described by J. Arrand, P. Myers, and R. J. Roberts (unpublished data). A mutant of S. albus G was isolated which was defective in both restriction and modification of Pal6. This mutant lacked SalI activity. It is concluded that SalI is the agent of restriction of Pal6 by S. albus G.", "contents": "Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI. The bacteriophage Pa16, isolated from soil on Streptomyces albus G, was restricted when transferred from an alternative host back to S. albus G. Extracted unmodified Pa16 deoxyribonucleic acid was cleaved at a single site by a cell-free extract of S. albus G. Fractions cleaving Pal6 deoxyribonucleic acid contained the endonuclease SalI first described by J. Arrand, P. Myers, and R. J. Roberts (unpublished data). A mutant of S. albus G was isolated which was defective in both restriction and modification of Pal6. This mutant lacked SalI activity. It is concluded that SalI is the agent of restriction of Pal6 by S. albus G.", "PMID": 977549}
{"id": "pubmed23n0032_11147", "title": "Cytochemical evidence of extramembranous carbohydrates on Ureaplasma urealyticum (T-strain Mycoplasma).", "content": "Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues.", "contents": "Cytochemical evidence of extramembranous carbohydrates on Ureaplasma urealyticum (T-strain Mycoplasma). Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues.", "PMID": 977550}
{"id": "pubmed23n0032_11148", "title": "Dermatan sulfate-chondroitin sulfate copolymers from ambilical cord. Isolation and characterization.", "content": "Dermatan sulfate-chondroitin sulfate copolymers have been isolated from human umbilical cord as a major galactosaminoglycan component of this tissue. The galactosaminoglycan fraction was obtained from this tissue by papain [EC 3.4.22.2] digestion followed by precipitation with cetylpyridinium chloride in a yield of 700 mg per 100 g of dry tissue. Ethanol fractionation resolved 4-5 subfractions differing in relative content of L-iduronic acid and D-glucuronic acid. No galactosaminoglycan containing either solely L-iduronic acid or D-glucuronic acid was obtained. The copolymeric structure of the material in each subfraction was demonstrated by analysis of oligosaccharide fragments obtained by chondroitinase-AC [EC 4.2.2.5] digestion. All the polymers contained repeating disaccharide units, D-glucuronosyl-N-acetylgalactosamine, D-glucuronosyl-N-acetylgalactosamine 4-sulfate, D-glucuronosyl-N-acetyl-galactosamine 6-sulfate, and L-iduronosyl-N-acetylgalactosamine 4-sulfate, of which D-glucuronosyl-N-acetylgalactosamine 6-sulfate and L-iduronosyl-N-acetylgalactosamine 4-sulfate were predominant. Both iduronic acid- and glucuronic acid-containing units were arranged in clusters. The presence of a considerable amount of nonsulfated disaccharide units was noted. The copolymers show extensive polydispersity in electrophoresis on cellulose acetate and gel chromatography on Sephadex G-200.", "contents": "Dermatan sulfate-chondroitin sulfate copolymers from ambilical cord. Isolation and characterization. Dermatan sulfate-chondroitin sulfate copolymers have been isolated from human umbilical cord as a major galactosaminoglycan component of this tissue. The galactosaminoglycan fraction was obtained from this tissue by papain [EC 3.4.22.2] digestion followed by precipitation with cetylpyridinium chloride in a yield of 700 mg per 100 g of dry tissue. Ethanol fractionation resolved 4-5 subfractions differing in relative content of L-iduronic acid and D-glucuronic acid. No galactosaminoglycan containing either solely L-iduronic acid or D-glucuronic acid was obtained. The copolymeric structure of the material in each subfraction was demonstrated by analysis of oligosaccharide fragments obtained by chondroitinase-AC [EC 4.2.2.5] digestion. All the polymers contained repeating disaccharide units, D-glucuronosyl-N-acetylgalactosamine, D-glucuronosyl-N-acetylgalactosamine 4-sulfate, D-glucuronosyl-N-acetyl-galactosamine 6-sulfate, and L-iduronosyl-N-acetylgalactosamine 4-sulfate, of which D-glucuronosyl-N-acetylgalactosamine 6-sulfate and L-iduronosyl-N-acetylgalactosamine 4-sulfate were predominant. Both iduronic acid- and glucuronic acid-containing units were arranged in clusters. The presence of a considerable amount of nonsulfated disaccharide units was noted. The copolymers show extensive polydispersity in electrophoresis on cellulose acetate and gel chromatography on Sephadex G-200.", "PMID": 977551}
{"id": "pubmed23n0032_11149", "title": "Further studies on the possible compartmentation of the precursor pool of cholesterol for the biosynthesis of cholic acid in the rat.", "content": "In vivo and in vitro experiments with rats were carried out to investie precursor for the biosynthesis of cholic acid. When rats with a bile-fistula were given a mixture of [2-14C]mevalonate and [1,2-3H]cholesterol intravenously, the 14C:3H ratio in cholic acid in both whole homogenate and cytosol prepared from their lives was higher than that in free cholesterol in any subcellular fraction of the livers. When [2-14C] mevalonate was administered intravenously to bile-fistula rats, the specific radioactivity of free cholesterol in the hepatic microsomal fraction exceeded that in any other fraction, and the specific radioactivity of biliary cholic acid was remarkably high, exceeding that of microsomal free cholesterol. In similar experiments with [4-14C] cholesterol, the specific radioactivity of free cholesterol in the hepatic microsomal fraction exceeded that in any other subcellular fraction and the specific radioactivity of biliary cholic acid was lower than that of free cholesterol in any hepatic subcellular fraction. Tissue suspensions of rat livers in Krebs-Ringer bicarbonate (pH 7.4)-5.5 mM glucose were incubated with [2-14C]mevalonate in O2-CO2 (95:5, v/v) at 37 degrees. The specific radioactivity of free cholesterol in the microsomal fraction prepared from the incubated tissue exceeded the specific radioactivities of free cholesterol in the other subcellular fractions. The estimated specific radioactivity of taurocholate formed during the incubation was far higher than that of microsomal free cholesterol. These data indicate that hepatic microsomal free cholesterol which was newly synthesized in situ was preferentially incorporated into cholic acid.", "contents": "Further studies on the possible compartmentation of the precursor pool of cholesterol for the biosynthesis of cholic acid in the rat. In vivo and in vitro experiments with rats were carried out to investie precursor for the biosynthesis of cholic acid. When rats with a bile-fistula were given a mixture of [2-14C]mevalonate and [1,2-3H]cholesterol intravenously, the 14C:3H ratio in cholic acid in both whole homogenate and cytosol prepared from their lives was higher than that in free cholesterol in any subcellular fraction of the livers. When [2-14C] mevalonate was administered intravenously to bile-fistula rats, the specific radioactivity of free cholesterol in the hepatic microsomal fraction exceeded that in any other fraction, and the specific radioactivity of biliary cholic acid was remarkably high, exceeding that of microsomal free cholesterol. In similar experiments with [4-14C] cholesterol, the specific radioactivity of free cholesterol in the hepatic microsomal fraction exceeded that in any other subcellular fraction and the specific radioactivity of biliary cholic acid was lower than that of free cholesterol in any hepatic subcellular fraction. Tissue suspensions of rat livers in Krebs-Ringer bicarbonate (pH 7.4)-5.5 mM glucose were incubated with [2-14C]mevalonate in O2-CO2 (95:5, v/v) at 37 degrees. The specific radioactivity of free cholesterol in the microsomal fraction prepared from the incubated tissue exceeded the specific radioactivities of free cholesterol in the other subcellular fractions. The estimated specific radioactivity of taurocholate formed during the incubation was far higher than that of microsomal free cholesterol. These data indicate that hepatic microsomal free cholesterol which was newly synthesized in situ was preferentially incorporated into cholic acid.", "PMID": 977552}
{"id": "pubmed23n0032_11150", "title": "Determination of the best-fit values of kinetic parameters of the Michaelis-Menten equation by the method of least squares with the Taylor expansion.", "content": "The best-fit values of the Michaelis constant (Km) and the maximum velocity (V) in the Michaelis-Menten equation can be obtained by the method of least squares with the Taylor expansion for the sum of squares of the absolute residual, i.e., the difference between the observed velocity and the corresponding velocity by calculation. This method makes it possible to determine the values of Km and V not in a trial-and-error manner but in a deductive and unique manner after some iterative procedures starting from arbitrary approximate values of Km and V. These values can be said to be uniquely determined for a set of data as the finally converged values are no longer dependent upon the initial approximate values of Km and V. It is also very important to obtain initial approximate values of parameters for the application of the method described above. A simple method is proposed to estimate the approximate values of parameters involved in fractional functions. The method of rearrangement after canceling of denominator of a fractional function can be utilized to obtain approximate values, not only for cases of two unknown parameters such as the Michaelis-Menten equation, but also for cases with more than two unknowns.", "contents": "Determination of the best-fit values of kinetic parameters of the Michaelis-Menten equation by the method of least squares with the Taylor expansion. The best-fit values of the Michaelis constant (Km) and the maximum velocity (V) in the Michaelis-Menten equation can be obtained by the method of least squares with the Taylor expansion for the sum of squares of the absolute residual, i.e., the difference between the observed velocity and the corresponding velocity by calculation. This method makes it possible to determine the values of Km and V not in a trial-and-error manner but in a deductive and unique manner after some iterative procedures starting from arbitrary approximate values of Km and V. These values can be said to be uniquely determined for a set of data as the finally converged values are no longer dependent upon the initial approximate values of Km and V. It is also very important to obtain initial approximate values of parameters for the application of the method described above. A simple method is proposed to estimate the approximate values of parameters involved in fractional functions. The method of rearrangement after canceling of denominator of a fractional function can be utilized to obtain approximate values, not only for cases of two unknown parameters such as the Michaelis-Menten equation, but also for cases with more than two unknowns.", "PMID": 977553}
{"id": "pubmed23n0032_11151", "title": "Circadian rhythms of digestive enzymes in the small intestine of the rat. II. Effects of fasting and refeeding.", "content": "The effects of fasting were examined on the rhythmic changes in the activities of maltase [EC 3.2.1.20] and leucine aminopeptidase [EC 3.4.11.1] in the small intestine of rats which has been kept under scheduled feeding conditions. Irrespective of whether the rats had been kept on a daytime or nighttime feeding schedule, the rhythms of maltase and leucine aminopeptidase persisted when the animals were starved. However, the amplitude of the leucine aminopeptidase rhythm began to decrease from the first day of fasting, while that of maltase did not. Conspicuous rhythms persisted for at least 2 days during fasting, but they gradually became vague and disappeared after 5 days. When rats were refed after fasting, the leucine aminopeptidase activity increased within a few hours, but the maltose activity did not. It is suggested that the rhythms of the digestive enzymes in the small intestine of rats are not a direct consequence of food intake, but are triggered off by the anticipatory mechanism which operates when rats expect to be fed. The rhythmic change of leucine aminopeptidase seemed to be intensified by food intake.", "contents": "Circadian rhythms of digestive enzymes in the small intestine of the rat. II. Effects of fasting and refeeding. The effects of fasting were examined on the rhythmic changes in the activities of maltase [EC 3.2.1.20] and leucine aminopeptidase [EC 3.4.11.1] in the small intestine of rats which has been kept under scheduled feeding conditions. Irrespective of whether the rats had been kept on a daytime or nighttime feeding schedule, the rhythms of maltase and leucine aminopeptidase persisted when the animals were starved. However, the amplitude of the leucine aminopeptidase rhythm began to decrease from the first day of fasting, while that of maltase did not. Conspicuous rhythms persisted for at least 2 days during fasting, but they gradually became vague and disappeared after 5 days. When rats were refed after fasting, the leucine aminopeptidase activity increased within a few hours, but the maltose activity did not. It is suggested that the rhythms of the digestive enzymes in the small intestine of rats are not a direct consequence of food intake, but are triggered off by the anticipatory mechanism which operates when rats expect to be fed. The rhythmic change of leucine aminopeptidase seemed to be intensified by food intake.", "PMID": 977554}
{"id": "pubmed23n0032_11152", "title": "Isolation, properties, and crystallization of an iron-chlorin protein from Aspergillus niger.", "content": "A green iron-chlorin protein was purified 160-fold from a lyophilized extract of Aspergillus niger by ion-exchange chromatography and gel filtration with a yield of 25%. The purified preparation appeared nearly homogeneous on sedimentation analysis and the sedimentation coefficient of the protein at infinite dilution was 13.4S. Its molecular weight was calculated to be 2.8--3.2 X 10(5) from sedimentation and gel filtration data. The ferric form of the protein had absorption maxima at 587.5 and 708 nm in the visible region and a Soret band at 404 nm. High-spin ESR signals of a rhombically distorted ferric iron-chlorin complex were observed at g = 6.5 and 5.3 together with unidentified, weaker signals at g = 4.3 and 2.0. The ferric form reacted readily with cyanide to give a complex showing absorption peaks at 422 and 632 nm and a shoulder at about 595 nm. When the protein combined with cyanide its high-spin ESR signals disappeared and low-spin signals at g = 1.88, 2.29, anous form having absorption maxima at 622 and about 410 nm. The rate of reduction by dithionite was slightly reduced by the presence of either nitrite or sulfite, and greatly accelerated by the presence of hydroxylamine. The reduced spectrum obtained in the presence of hydroxylamine had maxima at 620 and about 420 nm. The ferric cyanide complex did not show any spectral change on addition of dithionite. The green prosthetic group could be extracted with acidified acetone and the absorption maxima of the pyridine ferrihemochrome were at 413 and 599 nm. On removal of metal from the prosthetic group the characteristic spectrum of a chlorin was obtained. The absorption maxima of a solution of the chlorin in benzene were at 403, 501, 537, 576, 595, and 655 nm, the 655 nm band being strongest of those in the visible region. No significant amount of flavin was detected in the purified preparation. The iron-chlorin protein catalyzed methyl viologen-linked reduction of hydroxylamine and also that of nitrite at a slower rate under the same conditions, but not evidence that it reduced sulfite was obtained in the present study. The purified preparation also had high catalase [ec 1.11.1.6] activity. Crystalline material was obtained by gradual concentration of the purified preparation at about pH 6.", "contents": "Isolation, properties, and crystallization of an iron-chlorin protein from Aspergillus niger. A green iron-chlorin protein was purified 160-fold from a lyophilized extract of Aspergillus niger by ion-exchange chromatography and gel filtration with a yield of 25%. The purified preparation appeared nearly homogeneous on sedimentation analysis and the sedimentation coefficient of the protein at infinite dilution was 13.4S. Its molecular weight was calculated to be 2.8--3.2 X 10(5) from sedimentation and gel filtration data. The ferric form of the protein had absorption maxima at 587.5 and 708 nm in the visible region and a Soret band at 404 nm. High-spin ESR signals of a rhombically distorted ferric iron-chlorin complex were observed at g = 6.5 and 5.3 together with unidentified, weaker signals at g = 4.3 and 2.0. The ferric form reacted readily with cyanide to give a complex showing absorption peaks at 422 and 632 nm and a shoulder at about 595 nm. When the protein combined with cyanide its high-spin ESR signals disappeared and low-spin signals at g = 1.88, 2.29, anous form having absorption maxima at 622 and about 410 nm. The rate of reduction by dithionite was slightly reduced by the presence of either nitrite or sulfite, and greatly accelerated by the presence of hydroxylamine. The reduced spectrum obtained in the presence of hydroxylamine had maxima at 620 and about 420 nm. The ferric cyanide complex did not show any spectral change on addition of dithionite. The green prosthetic group could be extracted with acidified acetone and the absorption maxima of the pyridine ferrihemochrome were at 413 and 599 nm. On removal of metal from the prosthetic group the characteristic spectrum of a chlorin was obtained. The absorption maxima of a solution of the chlorin in benzene were at 403, 501, 537, 576, 595, and 655 nm, the 655 nm band being strongest of those in the visible region. No significant amount of flavin was detected in the purified preparation. The iron-chlorin protein catalyzed methyl viologen-linked reduction of hydroxylamine and also that of nitrite at a slower rate under the same conditions, but not evidence that it reduced sulfite was obtained in the present study. The purified preparation also had high catalase [ec 1.11.1.6] activity. Crystalline material was obtained by gradual concentration of the purified preparation at about pH 6.", "PMID": 977555}
{"id": "pubmed23n0032_11153", "title": "New types of gangliosides in starfish with sialic acid residues in the inner part of their carbohydrate chains.", "content": "Three gangliosides were isolated from the starfish by Folch's partition, dialysis, silicic acid column chromatography, Iatrobead column chromatography and preparative thin-layer chromatography. Partial acid hydrolysis of the three lipids in 0.1 N HCl at 80 degrees for 90 min liberated all the lactosyl ceramide and water-soluble degradation products, but no sialic acid. Gas-liquid chromatography of the trimethylsilylated methyl glycosides of the water-soluble products demonstrated the presence of arabinose in addition to the usual sugar units of gangliosides.", "contents": "New types of gangliosides in starfish with sialic acid residues in the inner part of their carbohydrate chains. Three gangliosides were isolated from the starfish by Folch's partition, dialysis, silicic acid column chromatography, Iatrobead column chromatography and preparative thin-layer chromatography. Partial acid hydrolysis of the three lipids in 0.1 N HCl at 80 degrees for 90 min liberated all the lactosyl ceramide and water-soluble degradation products, but no sialic acid. Gas-liquid chromatography of the trimethylsilylated methyl glycosides of the water-soluble products demonstrated the presence of arabinose in addition to the usual sugar units of gangliosides.", "PMID": 977556}
{"id": "pubmed23n0032_11154", "title": "Quantitative analysis of the action of Taka-amylase A on maltotriose.", "content": "The action of Taka-amylase A from Asp. oryzae was studied quantitatively by the product analysis method using unlabeled maltotriose and maltotriose labeled at the reducing end as substrates. It was found that the ratio of the unlabeled products, maltose (G2) and glucose (G1) exceeded unity, and that the ratio of the labeled products, G2/G1 was strongly dependent on the initial substrate concentrations. The results can only be explained by a transglycosylation or condensation mechanism or both. Analysis of maltose labeled at the nonreducing or reducing end reveal that the ratio of the transglycosylation to the condensation mechanism was about 20:1 with about 80 mM maltotriose. A computer simulation was made on a reaction scheme involving the termolecular-shifted complex, transglycosylation and condensation besides hydrolysis, by using reported subsite affinities as modified by the authors. The simulation reproduced the experimental results satisfactorily.", "contents": "Quantitative analysis of the action of Taka-amylase A on maltotriose. The action of Taka-amylase A from Asp. oryzae was studied quantitatively by the product analysis method using unlabeled maltotriose and maltotriose labeled at the reducing end as substrates. It was found that the ratio of the unlabeled products, maltose (G2) and glucose (G1) exceeded unity, and that the ratio of the labeled products, G2/G1 was strongly dependent on the initial substrate concentrations. The results can only be explained by a transglycosylation or condensation mechanism or both. Analysis of maltose labeled at the nonreducing or reducing end reveal that the ratio of the transglycosylation to the condensation mechanism was about 20:1 with about 80 mM maltotriose. A computer simulation was made on a reaction scheme involving the termolecular-shifted complex, transglycosylation and condensation besides hydrolysis, by using reported subsite affinities as modified by the authors. The simulation reproduced the experimental results satisfactorily.", "PMID": 977557}
{"id": "pubmed23n0032_11155", "title": "A clottable protein (coagulogen) of horseshoe crab hemocytes. Structural change of its polypeptide chain during gel formation.", "content": "A clottable protein (coagulogen) isolated from a hemocyte lysate of the Japanese horseshoe crab (Tachypleus tridentatus) was incubated with an endotoxin-activated clotting enzyme(s) partially purified from the same lysate, and its structural change during gel formation was examined. The results indicated that the enzymatic formation of gel involved limited proteolysis of the Arg-Gly and Arg-Thr linkages located in the N-terminal portion of the coagulogen, liberating peptide C.", "contents": "A clottable protein (coagulogen) of horseshoe crab hemocytes. Structural change of its polypeptide chain during gel formation. A clottable protein (coagulogen) isolated from a hemocyte lysate of the Japanese horseshoe crab (Tachypleus tridentatus) was incubated with an endotoxin-activated clotting enzyme(s) partially purified from the same lysate, and its structural change during gel formation was examined. The results indicated that the enzymatic formation of gel involved limited proteolysis of the Arg-Gly and Arg-Thr linkages located in the N-terminal portion of the coagulogen, liberating peptide C.", "PMID": 977558}
{"id": "pubmed23n0032_11156", "title": "Photoelectrochemical effects in the electrolyte-pigment-metal system. I. Metal-free phthalocyanine film description of short-circuit photocurrents for thin films of pigment.", "content": "Using a semitransparent metallic electrode covered with a thin film of metal-free phthalocyanine, photoelectrochemical effects have been studied in the electrolyte-pigment-metal system. Measurement of the short-circuit photocurrent, produced by continuous illumination, allows description of a photodependent kinetic phase corresponding to the establishment of a stationary state in the charge separation region of the system after the creation of the current carriers.", "contents": "Photoelectrochemical effects in the electrolyte-pigment-metal system. I. Metal-free phthalocyanine film description of short-circuit photocurrents for thin films of pigment. Using a semitransparent metallic electrode covered with a thin film of metal-free phthalocyanine, photoelectrochemical effects have been studied in the electrolyte-pigment-metal system. Measurement of the short-circuit photocurrent, produced by continuous illumination, allows description of a photodependent kinetic phase corresponding to the establishment of a stationary state in the charge separation region of the system after the creation of the current carriers.", "PMID": 977559}
{"id": "pubmed23n0032_11157", "title": "Photoelectrochemical effects in the electrolyte-pigment-metal system. II. Metal-free phthalocyanine film action spectra of short-circuit photocurrents with increase of the film thickness.", "content": "The electrolyte-pigment-metal system can be described as analogous to a photosensitive junction region. When the thickness of the pigment film is increased, the action spectra of the maximum short-circuit photo-current under continuous illumination differ from the absorption spectra both in direct illumination (pigment-electrolyte) and in back illumination (metal-pigment). One is led to believe that there exist two photoactive regions in the system for the production of the short-circuit photocurrent; these two active regions are associated respectively with each interface. When the metallic semitransparent electrode is made of aluminum the two interfaces have opposite sign contributions to the photocurrent; this allows the determination of conditions in which one can observe specifically the contribution of the pigment-electrolyte interface, that is the interactions between excited pigment molecules and the redox system in the electrolyte.", "contents": "Photoelectrochemical effects in the electrolyte-pigment-metal system. II. Metal-free phthalocyanine film action spectra of short-circuit photocurrents with increase of the film thickness. The electrolyte-pigment-metal system can be described as analogous to a photosensitive junction region. When the thickness of the pigment film is increased, the action spectra of the maximum short-circuit photo-current under continuous illumination differ from the absorption spectra both in direct illumination (pigment-electrolyte) and in back illumination (metal-pigment). One is led to believe that there exist two photoactive regions in the system for the production of the short-circuit photocurrent; these two active regions are associated respectively with each interface. When the metallic semitransparent electrode is made of aluminum the two interfaces have opposite sign contributions to the photocurrent; this allows the determination of conditions in which one can observe specifically the contribution of the pigment-electrolyte interface, that is the interactions between excited pigment molecules and the redox system in the electrolyte.", "PMID": 977560}
{"id": "pubmed23n0032_11158", "title": "Photoelectrochemical effects in the electrolyte-pigment-metal system. III. Chlorophyll films short-circuit photocurrent transients light energy conversion efficiency.", "content": "Short-circuit photocurrents produced by a semitransparent metallic electrode covered with multilayers of chlorophyll and immersed in an electrolyte have been studied. The action spectrum of maximum photocurrent is identical to the absorption spectrum of the film of pigment. The kinetics of rise of the photocurrent are photoindependent for thin multilayers but dependent on thickness. Comparison of efficiency of light energy conversion on the basis of short-circuit photocurrent seems to show that the stack of Chl a monolayers is the more efficient chlorophyll solid system. Introduction for the same number of Chl a molecules of vitamin K1 and Phytol shows that vitamin K1 acts probably like an excitation quencher as in monolayers at the water-air interface.", "contents": "Photoelectrochemical effects in the electrolyte-pigment-metal system. III. Chlorophyll films short-circuit photocurrent transients light energy conversion efficiency. Short-circuit photocurrents produced by a semitransparent metallic electrode covered with multilayers of chlorophyll and immersed in an electrolyte have been studied. The action spectrum of maximum photocurrent is identical to the absorption spectrum of the film of pigment. The kinetics of rise of the photocurrent are photoindependent for thin multilayers but dependent on thickness. Comparison of efficiency of light energy conversion on the basis of short-circuit photocurrent seems to show that the stack of Chl a monolayers is the more efficient chlorophyll solid system. Introduction for the same number of Chl a molecules of vitamin K1 and Phytol shows that vitamin K1 acts probably like an excitation quencher as in monolayers at the water-air interface.", "PMID": 977561}
{"id": "pubmed23n0032_11159", "title": "Chemical models of oxidative phosphorylation.", "content": "Chemical models for coupling oxidation to phosphorylation are summarized and examined both from the standpoint of organic reaction mechanisms and with respect to their relevance to mitochondria and chloroplasts. In order to accelerate the progress of our research in bioenergetics, it is suggested to focus at least as much attention on structural biochemistry as on phenomenological observations of energy-transducing membranes.", "contents": "Chemical models of oxidative phosphorylation. Chemical models for coupling oxidation to phosphorylation are summarized and examined both from the standpoint of organic reaction mechanisms and with respect to their relevance to mitochondria and chloroplasts. In order to accelerate the progress of our research in bioenergetics, it is suggested to focus at least as much attention on structural biochemistry as on phenomenological observations of energy-transducing membranes.", "PMID": 977562}
{"id": "pubmed23n0032_11160", "title": "Ecto-enzymes of mammary gland and its tumors. Lectin inhibition of 5'-nucleotidase of the 13762 rat mammary ascites carcinoma.", "content": "Lectin inactivation of the cell surface enzyme 5'-nucleotidase has been studied in intact 13762 mammary ascites cells, cell membrane envelopes, membrane vesicles, and solubilized membranes as a means of understanding the effects of lectins on biochemical processes and the behavior of the enzyme in the membranes. The properties of the enzyme are essentially the same in the intact cells and in membrane envelopes prepared after zinc treatment of the cells. The enzyme has a Km of 25 muM and is inhibited by concanavalin A (Con A) by an apparent noncompetitive process, which is reversed by alpha-methylmannoside. Half-maximal inhibition requires about 50 mug of Con A per mg of membrane protein. The inhibition for the intact cells and membrane envelopes does not exhibit significant cooperativity. Membrane vesicles, prepared by EDTA extraction, and solubilized membranes show differences in the behavior of the nucleotidase toward Con A even though the Km of the enzyme (25 muM) and inactivation (noncompetitive) are essentially unchanged. The Hill coefficient for the inactivation process in solubilized membranes and membrane vesicles is shifted to near 2, indicating significant cooperativity in the Con A-enzyme interaction. It is suggested that this change in cooperativity may reflect a different mode of interaction with the membrane and increased enzyme mobility, although an alteration in enzyme subunit interactions cannot be ruled out at present.", "contents": "Ecto-enzymes of mammary gland and its tumors. Lectin inhibition of 5'-nucleotidase of the 13762 rat mammary ascites carcinoma. Lectin inactivation of the cell surface enzyme 5'-nucleotidase has been studied in intact 13762 mammary ascites cells, cell membrane envelopes, membrane vesicles, and solubilized membranes as a means of understanding the effects of lectins on biochemical processes and the behavior of the enzyme in the membranes. The properties of the enzyme are essentially the same in the intact cells and in membrane envelopes prepared after zinc treatment of the cells. The enzyme has a Km of 25 muM and is inhibited by concanavalin A (Con A) by an apparent noncompetitive process, which is reversed by alpha-methylmannoside. Half-maximal inhibition requires about 50 mug of Con A per mg of membrane protein. The inhibition for the intact cells and membrane envelopes does not exhibit significant cooperativity. Membrane vesicles, prepared by EDTA extraction, and solubilized membranes show differences in the behavior of the nucleotidase toward Con A even though the Km of the enzyme (25 muM) and inactivation (noncompetitive) are essentially unchanged. The Hill coefficient for the inactivation process in solubilized membranes and membrane vesicles is shifted to near 2, indicating significant cooperativity in the Con A-enzyme interaction. It is suggested that this change in cooperativity may reflect a different mode of interaction with the membrane and increased enzyme mobility, although an alteration in enzyme subunit interactions cannot be ruled out at present.", "PMID": 977563}
{"id": "pubmed23n0032_11161", "title": "Mechanism for the several activities of the glutathione S-transferases.", "content": "The catalyzed reactions of GSH with organic nitrate and thiocyanate esters and with a series of chloronitrobenzene substrates have been investigated and the results used to formulate a mechanism for glutathione S-transferase catalysis. All the homogeneous preparations of the glutathione transferases that have been tested catalyze the reaction of GSH with organic nitrates and thiocyanates. The nature of the reaction with nitrate esters, resulting in the formation of GSSG rather than a thioether, has been investigated further. The presence of an additional nonsubstrate thiol decreased the formation of GSSG to an extent that cannot be explained by disulfide interchange. These results are interpreted to reflect the enzymatic formation of an unstable glutathione sulfenyl nitrite that undergoes subsequent non-enzymatic decomposition. Hammett plots of the catalytic constants of rat liver transferases B and C obtained with a series of 4-substituted 1-chloro-2-nitrobenzene substrates demonstrate a linear relationship with sigma- substituent constants, reflecting the nucleophilic nature of the enzymatic reactions and their strong dependence on the electrophilicity of the nonthiol substrate. These data suggest that the many diverse reactions catalyzed by the glutathione transferases may be formulated as a nucleophilic attack of enzyme-bound GSH on the electrophilic center of the second substrate. The final products observed reflect this primary event and the existence of subsequent nonenzymatic reactions.", "contents": "Mechanism for the several activities of the glutathione S-transferases. The catalyzed reactions of GSH with organic nitrate and thiocyanate esters and with a series of chloronitrobenzene substrates have been investigated and the results used to formulate a mechanism for glutathione S-transferase catalysis. All the homogeneous preparations of the glutathione transferases that have been tested catalyze the reaction of GSH with organic nitrates and thiocyanates. The nature of the reaction with nitrate esters, resulting in the formation of GSSG rather than a thioether, has been investigated further. The presence of an additional nonsubstrate thiol decreased the formation of GSSG to an extent that cannot be explained by disulfide interchange. These results are interpreted to reflect the enzymatic formation of an unstable glutathione sulfenyl nitrite that undergoes subsequent non-enzymatic decomposition. Hammett plots of the catalytic constants of rat liver transferases B and C obtained with a series of 4-substituted 1-chloro-2-nitrobenzene substrates demonstrate a linear relationship with sigma- substituent constants, reflecting the nucleophilic nature of the enzymatic reactions and their strong dependence on the electrophilicity of the nonthiol substrate. These data suggest that the many diverse reactions catalyzed by the glutathione transferases may be formulated as a nucleophilic attack of enzyme-bound GSH on the electrophilic center of the second substrate. The final products observed reflect this primary event and the existence of subsequent nonenzymatic reactions.", "PMID": 977564}
{"id": "pubmed23n0032_11162", "title": "Purification of a glycoprotein from mouse ascites fluid by immunoaffinity chromatography which is related to the major glycoprotein of murine leukemia viruses. Immunologic and structural comparison with purified viral glycoproteins.", "content": "Proteins immunologically related to the major glycoprotein from murine leukemia viruses, gp70, are expressed in the tissue and body fluids of unmanipulated mice in the absence of virus particle production. Cross-reactive gp70 was isolated from ascites fluid of NZB and (NZB X NZW)F1 mice utilizing immunoaffinity chromatography. An enriched antibody preparation bound to Sepharose was used as an immunosorbent to purify gp70 over 600-fold in one step with a 30% recovery. The gp70s from ascites fluid were compared with gp70s purified from laboratory virus strains Friend, Rauscher, Moloney, and Scripps leukemia viruses. Direct antibody binding studies showed that the viral gp70s were more closely related to each other than to the gp70s from ascites fluid. Furthermore, the gp70s from ascites fluid were more reactive with antibody to xenotropic virus than were the gp70s from the laboratory viruses tested. Tryptic peptide map analysis of radioiodinated proteins showed that gp70 from Scripps virus is closely related to gp70 from Moloney virus in primary structure and less closely related structurally to gp70 from Rauscher virus and gp70 from NZB ascites fluid.", "contents": "Purification of a glycoprotein from mouse ascites fluid by immunoaffinity chromatography which is related to the major glycoprotein of murine leukemia viruses. Immunologic and structural comparison with purified viral glycoproteins. Proteins immunologically related to the major glycoprotein from murine leukemia viruses, gp70, are expressed in the tissue and body fluids of unmanipulated mice in the absence of virus particle production. Cross-reactive gp70 was isolated from ascites fluid of NZB and (NZB X NZW)F1 mice utilizing immunoaffinity chromatography. An enriched antibody preparation bound to Sepharose was used as an immunosorbent to purify gp70 over 600-fold in one step with a 30% recovery. The gp70s from ascites fluid were compared with gp70s purified from laboratory virus strains Friend, Rauscher, Moloney, and Scripps leukemia viruses. Direct antibody binding studies showed that the viral gp70s were more closely related to each other than to the gp70s from ascites fluid. Furthermore, the gp70s from ascites fluid were more reactive with antibody to xenotropic virus than were the gp70s from the laboratory viruses tested. Tryptic peptide map analysis of radioiodinated proteins showed that gp70 from Scripps virus is closely related to gp70 from Moloney virus in primary structure and less closely related structurally to gp70 from Rauscher virus and gp70 from NZB ascites fluid.", "PMID": 977565}
{"id": "pubmed23n0032_11163", "title": "Methylisocyanate as an antisickling agent and its reaction with hemoglobin S.", "content": "Reviewing the reaction of potassium cyanate, an antisickling agent, with alpha-amino groups of hemoglobin, it was found that the reaction was a slow process and requires a large excess of the reagent. The reason for the slow reaction rate of carbamylation of hemoglobin by cyanate is that cyanate itself does not react with hemoglobin. It is rather isocyanic acid, the reactive species, that reacts with hemoglobin. Since the pK of isocyanic acid is 3.8, only one out of 4,000 cyanate ions is present as isocyanic acid at physiological pH. Therefore, it appears that a large excess of cyanate ions is required to achieve the carbamylation of hemoglobin S, both in vivo and in vitro. Furthermore, the pH optimum for carbamylation of carboxyhemoglobin and deoxyhemoglobin is 5.5 and, at pH 7.4, the reaction velocity drops to one-third for carboxyhemoglobin and one-half for deoxyhemoglobin. To seek an approach to reduce the dosage of cyanate and to increase the reaction velocity, an isocyanate derivative, methylisocyanate which is already in the reactive form, was tested for its antisickling activity and its reaction with hemoglobin S. It was found that methylisocyanate had antisickling activity and that only a stoichiometric amount to 2-fold excess of the reagent over hemoglobin S alpha-amino groups was required to prevent the sickling of erythrocytes. Methylisocyanate-treated sickle erythrocytes showed an increased oxygen affinity compared to untreated methylisocyanate reacted with alpha-amino groups of hemoglobin S and the reaction was complete in less than 1 min. Methylcarbamylated hemoglobin S had a higher minimum gelling concentration than the untreated hemoglobin S. There was no detectable reaction of free sulfhydryl and epsilon-amino groups of hemoglobin S with methylisocyanate. These results indicate that methylisocyanate, and probably other isocyanate derivatives, possesses powerful antisickling activity.", "contents": "Methylisocyanate as an antisickling agent and its reaction with hemoglobin S. Reviewing the reaction of potassium cyanate, an antisickling agent, with alpha-amino groups of hemoglobin, it was found that the reaction was a slow process and requires a large excess of the reagent. The reason for the slow reaction rate of carbamylation of hemoglobin by cyanate is that cyanate itself does not react with hemoglobin. It is rather isocyanic acid, the reactive species, that reacts with hemoglobin. Since the pK of isocyanic acid is 3.8, only one out of 4,000 cyanate ions is present as isocyanic acid at physiological pH. Therefore, it appears that a large excess of cyanate ions is required to achieve the carbamylation of hemoglobin S, both in vivo and in vitro. Furthermore, the pH optimum for carbamylation of carboxyhemoglobin and deoxyhemoglobin is 5.5 and, at pH 7.4, the reaction velocity drops to one-third for carboxyhemoglobin and one-half for deoxyhemoglobin. To seek an approach to reduce the dosage of cyanate and to increase the reaction velocity, an isocyanate derivative, methylisocyanate which is already in the reactive form, was tested for its antisickling activity and its reaction with hemoglobin S. It was found that methylisocyanate had antisickling activity and that only a stoichiometric amount to 2-fold excess of the reagent over hemoglobin S alpha-amino groups was required to prevent the sickling of erythrocytes. Methylisocyanate-treated sickle erythrocytes showed an increased oxygen affinity compared to untreated methylisocyanate reacted with alpha-amino groups of hemoglobin S and the reaction was complete in less than 1 min. Methylcarbamylated hemoglobin S had a higher minimum gelling concentration than the untreated hemoglobin S. There was no detectable reaction of free sulfhydryl and epsilon-amino groups of hemoglobin S with methylisocyanate. These results indicate that methylisocyanate, and probably other isocyanate derivatives, possesses powerful antisickling activity.", "PMID": 977566}
{"id": "pubmed23n0032_11164", "title": "Isolation and characterization of rat olfactory marker protein.", "content": "The olfactory marker protein was isolated and characterized from rat olfactory bulbs. Its properties and those of the olfactory marker protein isolated from the mouse are described. The rat protein was less acidic (pI = 5.0) than the mouse protein (pI = 4.7). However, the amino acid compositions were very similar: in both proteins arginine plus lysine accounted for 13 mol% and glutamate plus aspartate for 30 mol% of the total residues. Molecular weights of both proteins estimated by sodium dodecyl sulfate gel electrophoresis were indistinguishable and estimated to be 16,500. The molecular weight of the native rat olfactory marker protein estimated by gel filtration techniques was 30,000, which is identical to the molecular weight of the native mouse and garfish olfactory marker proteins. This suggested a dimeric structure. The purified rat and mouse proteins behaved like species of 35,000 molecular weight on gel filtration.", "contents": "Isolation and characterization of rat olfactory marker protein. The olfactory marker protein was isolated and characterized from rat olfactory bulbs. Its properties and those of the olfactory marker protein isolated from the mouse are described. The rat protein was less acidic (pI = 5.0) than the mouse protein (pI = 4.7). However, the amino acid compositions were very similar: in both proteins arginine plus lysine accounted for 13 mol% and glutamate plus aspartate for 30 mol% of the total residues. Molecular weights of both proteins estimated by sodium dodecyl sulfate gel electrophoresis were indistinguishable and estimated to be 16,500. The molecular weight of the native rat olfactory marker protein estimated by gel filtration techniques was 30,000, which is identical to the molecular weight of the native mouse and garfish olfactory marker proteins. This suggested a dimeric structure. The purified rat and mouse proteins behaved like species of 35,000 molecular weight on gel filtration.", "PMID": 977567}
{"id": "pubmed23n0032_11165", "title": "Vitamin K-dependent carboxylase. Solubilization and properties.", "content": "Vitamin K is required for an enzymatic carboxylation of glutamyl residues in a microsomal protein precursor of plasma prothrombin to form gamma-carboxyglutamic acid. The enzyme system (carboxylase) which catalyzes this reaction has now been solubilized by extraction of the microsomes with Triton X-100 and has been shown to fix H14CO3- as gamma-carboxyglutamic acid residues in biologically active prothrombin. Enzyme activity requires O2 and vitamin K hydroquinone or vitamin K + NADH. Unlike the microsomal-bound carboxylase, soluble carboxylase activity is independent of either ATP or Mg2+ addition and is unaffected by either the ATP analog, adenyl-5'-yl imidodiphosphate (AMP-P(NH)P, or EDTA. These observations suggest that the energy required to drive the carboxylation reaction is derived from the oxidation of the reduced form of vitamin K. Although the membrane-bound carboxylase is inhibited by Warfarin, this anticoagulant is ineffective as an inhibitor of the soluble enzyme. A second anticoagulant, 2-chloro-3-phytyl-1,4-natpthoquinone (chloro-K), differs from Warfarin in that it effectively inhibits both the membrane-bound and soluble carboxylases.", "contents": "Vitamin K-dependent carboxylase. Solubilization and properties. Vitamin K is required for an enzymatic carboxylation of glutamyl residues in a microsomal protein precursor of plasma prothrombin to form gamma-carboxyglutamic acid. The enzyme system (carboxylase) which catalyzes this reaction has now been solubilized by extraction of the microsomes with Triton X-100 and has been shown to fix H14CO3- as gamma-carboxyglutamic acid residues in biologically active prothrombin. Enzyme activity requires O2 and vitamin K hydroquinone or vitamin K + NADH. Unlike the microsomal-bound carboxylase, soluble carboxylase activity is independent of either ATP or Mg2+ addition and is unaffected by either the ATP analog, adenyl-5'-yl imidodiphosphate (AMP-P(NH)P, or EDTA. These observations suggest that the energy required to drive the carboxylation reaction is derived from the oxidation of the reduced form of vitamin K. Although the membrane-bound carboxylase is inhibited by Warfarin, this anticoagulant is ineffective as an inhibitor of the soluble enzyme. A second anticoagulant, 2-chloro-3-phytyl-1,4-natpthoquinone (chloro-K), differs from Warfarin in that it effectively inhibits both the membrane-bound and soluble carboxylases.", "PMID": 977568}
{"id": "pubmed23n0032_11166", "title": "Interaction of manganese with bovine prothrombin and its thrombin-mediated cleavage products.", "content": "The binding of the paramagnetic metal, Mn(II), to bovine prothrombin and the thrombin-mediated cleavage products of prothrombin, i.e. fragment 1 and the prethrombin 1 has been investigated. Analysis of the Scatchard plots of the binding data reveals that prothrombin has two high affinity Mn(II) binding sites with a Kd of 1.2 +/- 1.0 X 10(-5) M and approximately two to three lower affinity Mn(II) sites with a Kd of 1.3 +/- 1.0 X 10(-4) M. Positive cooperativity in Mn(II) binding to prothrombin was observed for the strong sites. Fragment 1, the phospholipid-binding region of prothrombin, possesses two high affinity Mn(II) sites with a Kd of 2.2 +/- 1.0 X 10(-5) M and at least two lower affinity sites with a Kd of approximately 2.5 +/- 1.0 X 10(-4) M. Positive cooperativity was not observed for the binding of Mn(II) to fragment 1. Prethrombin 1 binds one Mn(II) with a Kd of 3.2 +/- 1.0 X 10(-4) M. Using the values of free Mn(II) concentration, as determined by EPR measurements and the observed enhancements of the water proton relaxation rates at various concentrations of Mn(II) and protein, the binary enhancement values (epsilon b) of the metal-protein complexes were obtained. The extrapolated values are 11 +/- 0.4 for the initial prothrombin-binding sites, and 10 +/- 0.3 for the tight binding sites of fragment 1. The unique epsilon b value obtained for prethrombin 1 was 5.3 +/- 0.7. When Mn(II) was used in a Factor Xa-metal ion-phospholipid system for activation of prothrombin, the rate of generation of thrombin was less than or equal to 5% of that obtained when Ca(II) was employed in this activation system. Addition of Mn(II) to the same activation system containing Ca(II) resulted in a marked decrease in the rate of thrombin generation, suggesting that Mn(II) probably competes for the same sites on prothrombin as Ca(II). In agreement with this is the observation that the Mn(II) sites on prothrombin could be displaced by Ca(II) at high concentrations of Ca(II).", "contents": "Interaction of manganese with bovine prothrombin and its thrombin-mediated cleavage products. The binding of the paramagnetic metal, Mn(II), to bovine prothrombin and the thrombin-mediated cleavage products of prothrombin, i.e. fragment 1 and the prethrombin 1 has been investigated. Analysis of the Scatchard plots of the binding data reveals that prothrombin has two high affinity Mn(II) binding sites with a Kd of 1.2 +/- 1.0 X 10(-5) M and approximately two to three lower affinity Mn(II) sites with a Kd of 1.3 +/- 1.0 X 10(-4) M. Positive cooperativity in Mn(II) binding to prothrombin was observed for the strong sites. Fragment 1, the phospholipid-binding region of prothrombin, possesses two high affinity Mn(II) sites with a Kd of 2.2 +/- 1.0 X 10(-5) M and at least two lower affinity sites with a Kd of approximately 2.5 +/- 1.0 X 10(-4) M. Positive cooperativity was not observed for the binding of Mn(II) to fragment 1. Prethrombin 1 binds one Mn(II) with a Kd of 3.2 +/- 1.0 X 10(-4) M. Using the values of free Mn(II) concentration, as determined by EPR measurements and the observed enhancements of the water proton relaxation rates at various concentrations of Mn(II) and protein, the binary enhancement values (epsilon b) of the metal-protein complexes were obtained. The extrapolated values are 11 +/- 0.4 for the initial prothrombin-binding sites, and 10 +/- 0.3 for the tight binding sites of fragment 1. The unique epsilon b value obtained for prethrombin 1 was 5.3 +/- 0.7. When Mn(II) was used in a Factor Xa-metal ion-phospholipid system for activation of prothrombin, the rate of generation of thrombin was less than or equal to 5% of that obtained when Ca(II) was employed in this activation system. Addition of Mn(II) to the same activation system containing Ca(II) resulted in a marked decrease in the rate of thrombin generation, suggesting that Mn(II) probably competes for the same sites on prothrombin as Ca(II). In agreement with this is the observation that the Mn(II) sites on prothrombin could be displaced by Ca(II) at high concentrations of Ca(II).", "PMID": 977569}
{"id": "pubmed23n0032_11167", "title": "Crystallization of proteins from polyethylene glycol.", "content": "Twenty-two proteins available to this laboratory were examined for their ability to crystallize from polyethylene glycol (PEG). Of the 22, 8 had been crystallized previously by conventional means and the remainder had yielded no crystals by other methods. Using a single broad screen with four different concentrations of five different molecular weight sizes of polyethylene glycol, 13 of these proteins were crystallized. Of the 13, 6 proteins were crystallized for the first time, one yielded crystals readily where only infrequent and irreproducible results were obtained before, and one other produced excellent quality crystals where only very disordered or twinned crystals were previously grown. In only one case did polyethylene glycol fail to yield crystals where other agents had succeeded. It is concluded that polyethylene glycol may be the best initial trial reagent for crystallization of proteins for x-ray diffraction analyses.", "contents": "Crystallization of proteins from polyethylene glycol. Twenty-two proteins available to this laboratory were examined for their ability to crystallize from polyethylene glycol (PEG). Of the 22, 8 had been crystallized previously by conventional means and the remainder had yielded no crystals by other methods. Using a single broad screen with four different concentrations of five different molecular weight sizes of polyethylene glycol, 13 of these proteins were crystallized. Of the 13, 6 proteins were crystallized for the first time, one yielded crystals readily where only infrequent and irreproducible results were obtained before, and one other produced excellent quality crystals where only very disordered or twinned crystals were previously grown. In only one case did polyethylene glycol fail to yield crystals where other agents had succeeded. It is concluded that polyethylene glycol may be the best initial trial reagent for crystallization of proteins for x-ray diffraction analyses.", "PMID": 977570}
{"id": "pubmed23n0032_11168", "title": "Bovine adrenal medulla troponin-C. Demonstration of a calcium-dependent conformational change.", "content": "A high affinity calcium-binding protein has recently been purified from the adrenal medulla (Kuo, I.C.Y., and Coffee, C.J. (1976) J. Biol. Chem. 251, 1603-1609). This protein is closely related in its chemical and physical properties to troponin-C (TN-C) of muscle tissue. Further examination of the adrenal medulla protein indicates that the removal of calcium is accompanied by a marked change in the conformation. This change in structure is similar, if not identical, to the calcium-dependent conformational change which has been described for skeletal muscle TN-C (Murray, A.C., and Kay, C.M. (1972) Biochemistry 11, 2622). The far ultraviolet circular dichroism spectrum of native adrenal medulla calcium-binding protein (AM-CBP) shows characteristic helical ellipticity bands at 222 and 207 nm. The helical content, as estimated from these data, is between 40 and 45%. Removal of calcium is accompanied by a change in ellipticity which corresponds to a decrease from 40 to 20% in the helical content. The near-ultraviolet circular dichroism spectrum shows negative dichroic bands at 262 and 268 nm which are characteristic of phenylalanine. These bands are relatively insensitive to changes in the calcium ion concentration. Sedimentation velocity studies likewise are indicative of a calcium-dependent structural alteration. The sedimentation coefficient of the native protein was observed to be 1.89 S. Similar measurements performed in the presence of 3 mM ethylene glycol bis(beta-aminoethyl ether) N,N, N', N'-tetraacetic acid (EGTA) gave a sedimentation coefficient of 1.50 S. The molecular weight, as determined by sedimentation equilibrium studies, was 16,000 regardless of whether the measurements were made in the presence of CaCl2 or EGTA. From the elution properties of AM-CBP on Sephadex G-100, the Stokes radius was observed to be 19.8 A in the presence of calcium and 21.9 A in the presence of EGTA. All of these changes which were induced by the addition of EGTA were completely reversible by the readdition of excess CaCl2. These data suggest that the removal of calcium from AM-CBP is accompanied by a pronounced conformational change which occurs without a molecular weight change. The decreased sedimentation coefficient, the increased Stokes radius, and the reduced helical content, which are observed for the apoprotein, indicate that removal of calcium results in a transformation from a compact symmetrical structure to one that is less ordered and more asymmetrical.", "contents": "Bovine adrenal medulla troponin-C. Demonstration of a calcium-dependent conformational change. A high affinity calcium-binding protein has recently been purified from the adrenal medulla (Kuo, I.C.Y., and Coffee, C.J. (1976) J. Biol. Chem. 251, 1603-1609). This protein is closely related in its chemical and physical properties to troponin-C (TN-C) of muscle tissue. Further examination of the adrenal medulla protein indicates that the removal of calcium is accompanied by a marked change in the conformation. This change in structure is similar, if not identical, to the calcium-dependent conformational change which has been described for skeletal muscle TN-C (Murray, A.C., and Kay, C.M. (1972) Biochemistry 11, 2622). The far ultraviolet circular dichroism spectrum of native adrenal medulla calcium-binding protein (AM-CBP) shows characteristic helical ellipticity bands at 222 and 207 nm. The helical content, as estimated from these data, is between 40 and 45%. Removal of calcium is accompanied by a change in ellipticity which corresponds to a decrease from 40 to 20% in the helical content. The near-ultraviolet circular dichroism spectrum shows negative dichroic bands at 262 and 268 nm which are characteristic of phenylalanine. These bands are relatively insensitive to changes in the calcium ion concentration. Sedimentation velocity studies likewise are indicative of a calcium-dependent structural alteration. The sedimentation coefficient of the native protein was observed to be 1.89 S. Similar measurements performed in the presence of 3 mM ethylene glycol bis(beta-aminoethyl ether) N,N, N', N'-tetraacetic acid (EGTA) gave a sedimentation coefficient of 1.50 S. The molecular weight, as determined by sedimentation equilibrium studies, was 16,000 regardless of whether the measurements were made in the presence of CaCl2 or EGTA. From the elution properties of AM-CBP on Sephadex G-100, the Stokes radius was observed to be 19.8 A in the presence of calcium and 21.9 A in the presence of EGTA. All of these changes which were induced by the addition of EGTA were completely reversible by the readdition of excess CaCl2. These data suggest that the removal of calcium from AM-CBP is accompanied by a pronounced conformational change which occurs without a molecular weight change. The decreased sedimentation coefficient, the increased Stokes radius, and the reduced helical content, which are observed for the apoprotein, indicate that removal of calcium results in a transformation from a compact symmetrical structure to one that is less ordered and more asymmetrical.", "PMID": 977571}
{"id": "pubmed23n0032_11169", "title": "Nicotinic acetylcholine receptor from rat brain. Solubilization, partial purification, and characterization.", "content": "Nicotinic acetylcholine receptor protein (nAChR) has been solubilized from rat cerebral cortices by extracting a crude membrane fraction with the nonionic detergent Triton X-100 (polyoxyethylene-p-t-octylphenol). The solubilized nAChR was partially purified by affinity chromatography (Naja naja siamensis alpha-toxin affinity arm, linked to Sepharose 4B) and characterized by binding of 125I-labeled alpha-bungarotoxin. The reaction of labeled toxin and nAChR appears to be second order with a rate constant (k1) equal to 0.38 X 10(5) M-1 S-1 at 20 degrees. The toxin-nAChR complex dissociates with a dissociation rate constant (k-1) of 1.23 X 10(-5) S-1 at 20 degrees (t 1/2 = 15.6 h). The kinetically determined dissociation constant (Kd) for the complex is 3.24 X 10(-10) M. A variety of cholinergic ligands were studied for their ability to inhibit binding of labeled toxin. The results indicate that the brain receptor is indeed nicotinic. The s20, w and v of the toxin-nAChR complex in 0.1% Triton were determined by velocity sedimentation in D2O and H2O sucrose gradients. The values are 12.9 S and 0.80 cm3 g-1. The Stokes radius of the complex determined by gel filtration equals 7.5 nm. The Mr of the complex calculated from the hydrodynamic parameters, and corrected for bound detergent, equals 357,000.", "contents": "Nicotinic acetylcholine receptor from rat brain. Solubilization, partial purification, and characterization. Nicotinic acetylcholine receptor protein (nAChR) has been solubilized from rat cerebral cortices by extracting a crude membrane fraction with the nonionic detergent Triton X-100 (polyoxyethylene-p-t-octylphenol). The solubilized nAChR was partially purified by affinity chromatography (Naja naja siamensis alpha-toxin affinity arm, linked to Sepharose 4B) and characterized by binding of 125I-labeled alpha-bungarotoxin. The reaction of labeled toxin and nAChR appears to be second order with a rate constant (k1) equal to 0.38 X 10(5) M-1 S-1 at 20 degrees. The toxin-nAChR complex dissociates with a dissociation rate constant (k-1) of 1.23 X 10(-5) S-1 at 20 degrees (t 1/2 = 15.6 h). The kinetically determined dissociation constant (Kd) for the complex is 3.24 X 10(-10) M. A variety of cholinergic ligands were studied for their ability to inhibit binding of labeled toxin. The results indicate that the brain receptor is indeed nicotinic. The s20, w and v of the toxin-nAChR complex in 0.1% Triton were determined by velocity sedimentation in D2O and H2O sucrose gradients. The values are 12.9 S and 0.80 cm3 g-1. The Stokes radius of the complex determined by gel filtration equals 7.5 nm. The Mr of the complex calculated from the hydrodynamic parameters, and corrected for bound detergent, equals 357,000.", "PMID": 977572}
{"id": "pubmed23n0032_11170", "title": "Studies on nicotinic acetylcholine receptors in mammalian brain. Preliminary characterization of membrane-bound alpha-bungarotoxin receptors in rat cerebral cortex.", "content": "Binding of alpha-bungarotoxin, labeled with 125I, has been studied in crude membrane preparations of rat cerebral cortex. Membrane-bound receptors appear to consist of at least two saturable subsets: one accounts for the binding of about 50 fmol of toxin/mg of protein, the other binds 120 fmol/mg. For the high affinity sites the dissociation constant for interaction of toxin and receptor extrapolated to zero protein concentration is 9.2 X 10(-10) M. Competition with a variety of agonists and antagonists indicates that toxin binding exhibits the pattern expected for nicotinic acetylcholine receptors. Toxin binding is inhibited by low concentrations of Ca2+ ions (50% inhibition at 3 mM) while higher concentrations are required for Mg2+, Na+, K+, and Li+ (50% inhibition at 0.5 M).", "contents": "Studies on nicotinic acetylcholine receptors in mammalian brain. Preliminary characterization of membrane-bound alpha-bungarotoxin receptors in rat cerebral cortex. Binding of alpha-bungarotoxin, labeled with 125I, has been studied in crude membrane preparations of rat cerebral cortex. Membrane-bound receptors appear to consist of at least two saturable subsets: one accounts for the binding of about 50 fmol of toxin/mg of protein, the other binds 120 fmol/mg. For the high affinity sites the dissociation constant for interaction of toxin and receptor extrapolated to zero protein concentration is 9.2 X 10(-10) M. Competition with a variety of agonists and antagonists indicates that toxin binding exhibits the pattern expected for nicotinic acetylcholine receptors. Toxin binding is inhibited by low concentrations of Ca2+ ions (50% inhibition at 3 mM) while higher concentrations are required for Mg2+, Na+, K+, and Li+ (50% inhibition at 0.5 M).", "PMID": 977573}
{"id": "pubmed23n0032_11171", "title": "Binding of chick skin collagen alpha 1 chain by isolated membranes from human platelets.", "content": "Binding of a denaturated polypeptide chain derived from chick skin collagen, the alpha 1(I) chain, by isolated membranes of human platelets has been demonstrated. The process is reversible, and time- and protein concentration-dependent. The binding is specific, with an association constant of 1.88 X 10(-6) M. Prior treatment of the isolated membranes with trypsin, chymotrypsin, and pronase, resulted in significant inhibition of the 14C-labeled alpha 1 chain binding, but neuraminidase or collagenase treatment had no effect. Dissociation of the bound radioactivity and subsequent chromatographic analyses on carboxymethylcellulose and agarose A-1.5m revealed that the alpha 1 chain was unaltered. Scatchard plot analysis suggested that there are approximately 20,000 binding sites per platelet. The binding of the alpha 1 chain was inhibited by a glycopeptide derived from alpha 1, alpha 1-CB5 and by purified glucosylgalactosyl hydroxylysine, but was not affected by other cyanogen bromide peptides of alpha 1, namely alpha 1-CB3, -CB4, -CB7, and -CB8. Kinetic studies demonstrated that inhibition by the hydroxylysine glycoside is competitive. Dose-response curves of platelet aggregation induced by alpha 1 and the binding of alpha 1 by platelet membranes correlate closely. These results indicate that there are specific binding sites for collagen alpha 1 chain on platelet membranes, and that the carbohydrate moiety of the alpha 1 chain plays a role in the binding. The findings also support the hypothesis that the chick skin alpha 1 chain mediates platelet aggregation and the release reaction by acting on platelet membranes.", "contents": "Binding of chick skin collagen alpha 1 chain by isolated membranes from human platelets. Binding of a denaturated polypeptide chain derived from chick skin collagen, the alpha 1(I) chain, by isolated membranes of human platelets has been demonstrated. The process is reversible, and time- and protein concentration-dependent. The binding is specific, with an association constant of 1.88 X 10(-6) M. Prior treatment of the isolated membranes with trypsin, chymotrypsin, and pronase, resulted in significant inhibition of the 14C-labeled alpha 1 chain binding, but neuraminidase or collagenase treatment had no effect. Dissociation of the bound radioactivity and subsequent chromatographic analyses on carboxymethylcellulose and agarose A-1.5m revealed that the alpha 1 chain was unaltered. Scatchard plot analysis suggested that there are approximately 20,000 binding sites per platelet. The binding of the alpha 1 chain was inhibited by a glycopeptide derived from alpha 1, alpha 1-CB5 and by purified glucosylgalactosyl hydroxylysine, but was not affected by other cyanogen bromide peptides of alpha 1, namely alpha 1-CB3, -CB4, -CB7, and -CB8. Kinetic studies demonstrated that inhibition by the hydroxylysine glycoside is competitive. Dose-response curves of platelet aggregation induced by alpha 1 and the binding of alpha 1 by platelet membranes correlate closely. These results indicate that there are specific binding sites for collagen alpha 1 chain on platelet membranes, and that the carbohydrate moiety of the alpha 1 chain plays a role in the binding. The findings also support the hypothesis that the chick skin alpha 1 chain mediates platelet aggregation and the release reaction by acting on platelet membranes.", "PMID": 977574}
{"id": "pubmed23n0032_11172", "title": "Nucleotide sequence of 5 S ribosomal ribonucleic acid of Iguana iguana.", "content": "Cell cultures of the reptile Iguana iguana have been labeled with 32PO4(3-) and the resulting radioactive 5 S rRNA isolated. Enzymatic digests of the radioactive RNA were fractionated by standard procedures, and the products of the digestions sequenced. From the sequences of the oligonucleotides produced by enzymatic digestion of the Iguana 5 S rRNA it is possible to propose a primary sequence for this RNA which differs in only two positions (2 and 25) from the sequence known for mammalian 5 S rRNAs. This sequence is closer to that of mammals than is that of any other nonmammalian species yet examined.", "contents": "Nucleotide sequence of 5 S ribosomal ribonucleic acid of Iguana iguana. Cell cultures of the reptile Iguana iguana have been labeled with 32PO4(3-) and the resulting radioactive 5 S rRNA isolated. Enzymatic digests of the radioactive RNA were fractionated by standard procedures, and the products of the digestions sequenced. From the sequences of the oligonucleotides produced by enzymatic digestion of the Iguana 5 S rRNA it is possible to propose a primary sequence for this RNA which differs in only two positions (2 and 25) from the sequence known for mammalian 5 S rRNAs. This sequence is closer to that of mammals than is that of any other nonmammalian species yet examined.", "PMID": 977575}
{"id": "pubmed23n0032_11173", "title": "Studies of 5'-nucleotidase in the perfused rat heart. Including measurements of the enzyme in perfused skeletal muscle and liver.", "content": "Perfused rat hearts catalyze the hydrolysis of AMP added to the perfusion fluid at a rate of 35 mumol/g dry weight/min. The activity is specific for 5'-nucleoside monophosphates, little activity being observed with 2' and 3'-AMP. The enzyme exhibits Michaelis-Menten kinetics in situ and is inhibited competitively by adenosine-5'-alpha, beta-methylene diphosphonate (Ki = 13 muM). This, as well as the nucleotide specificity, confirms that the hydrolysis is catalyzed by 5'-nucleotidase. The maximum activity of 5'-nucleotidase in perfused hearts is equal to or greater than that found in heart homogenates; thus, all of the enzyme is accessible to AMP added externally. Hydrolysis of endogenous AMP was studied in the perfused heart. Under aerobic conditions hearts contain very low amounts of purine nucleosides, and little or no nucleoside is found in the effluent perfusate. Under anaerobic conditions hearts accumulate adenosine, inosine, and hypoxanthine and release all three substances into the perfusate. Hydrolysis of externally added AMP was also observed in perfused skeletal muscle and liver, at rates of 10 and 17 mumol/g dry weight/min, respectively.", "contents": "Studies of 5'-nucleotidase in the perfused rat heart. Including measurements of the enzyme in perfused skeletal muscle and liver. Perfused rat hearts catalyze the hydrolysis of AMP added to the perfusion fluid at a rate of 35 mumol/g dry weight/min. The activity is specific for 5'-nucleoside monophosphates, little activity being observed with 2' and 3'-AMP. The enzyme exhibits Michaelis-Menten kinetics in situ and is inhibited competitively by adenosine-5'-alpha, beta-methylene diphosphonate (Ki = 13 muM). This, as well as the nucleotide specificity, confirms that the hydrolysis is catalyzed by 5'-nucleotidase. The maximum activity of 5'-nucleotidase in perfused hearts is equal to or greater than that found in heart homogenates; thus, all of the enzyme is accessible to AMP added externally. Hydrolysis of endogenous AMP was studied in the perfused heart. Under aerobic conditions hearts contain very low amounts of purine nucleosides, and little or no nucleoside is found in the effluent perfusate. Under anaerobic conditions hearts accumulate adenosine, inosine, and hypoxanthine and release all three substances into the perfusate. Hydrolysis of externally added AMP was also observed in perfused skeletal muscle and liver, at rates of 10 and 17 mumol/g dry weight/min, respectively.", "PMID": 977576}
{"id": "pubmed23n0032_11174", "title": "Protein synthesis in rabbit reticulocytes. Characteristics of mRNA (AUG codon)-dependent binding of Met-tRNAfMet to 40 S and 80 S ribosomes.", "content": "The characteristics of Met-tRNAfMet binding to ribosomes (40 S and 80 S) were studied using a two-stage assay method (Gupta, N.K., Chatterjee, B. and Majumdar, A. (1975) Biochem. Biophys. Res. Commun. 65, 797) and the complexes formed were analyzed either by Millipore filtration or by sucrose density gradient centrifugation. The results are summarized as follows: (a) with both assay methods, Met-tRNAfMet binding to 40 S ribosomes was entirely dependent upon addition of a partially purified mixture of initiation factors and AUG codon; (b) this binding occurred over a wide Mg2+ concentration range; significant binding was observed even at 20 mM Mg2+; (c) upon addition of 60 S ribosomes, a significant part of Met-tRNAfMet bound to 40 S ribosomes was transferred to 80 S complex. This transfer reaction had a sharp Mg2+ optimum around 2 mM. Met-tRNAfMet-80 S-AUG complex thus formed was active in Met-puromycin synthesis; (d) Met-tRNAfMet deacylase present in crude 0.5 M KCl ribosomal wash is a potent inhibitor of the binding reaction as it deacylates Met-tRNAfMet in the Met-tRNAfMet-40 S-AUG complex; (e) glutaraldehyde (0.5%) degrades Met-tRNAfMet-40 S-AUG complex but increases the background binding of Met-tRNAfMet to 40 S ribosomes in the absence of AUG codon; (f) polynucleotides containing uracil and adenosine are strong inhibitors of Met-tRNAfMet binding to 40 S ribosomes. The order of inhibitory activities of the polynucleotides tested was as follows: poly(rU)-poly(rA) (2:1) greater than poly(rU)-poly(rA) (1:1) greater than poly(rU) greater than poly(rA). Other RNAs tested such as poly(rC), poly(rI)-poly(rC) and phi6 bacteriophage RNA (double-stranded) were without significant effects on the Met-tRNAfMet-binding reaction.", "contents": "Protein synthesis in rabbit reticulocytes. Characteristics of mRNA (AUG codon)-dependent binding of Met-tRNAfMet to 40 S and 80 S ribosomes. The characteristics of Met-tRNAfMet binding to ribosomes (40 S and 80 S) were studied using a two-stage assay method (Gupta, N.K., Chatterjee, B. and Majumdar, A. (1975) Biochem. Biophys. Res. Commun. 65, 797) and the complexes formed were analyzed either by Millipore filtration or by sucrose density gradient centrifugation. The results are summarized as follows: (a) with both assay methods, Met-tRNAfMet binding to 40 S ribosomes was entirely dependent upon addition of a partially purified mixture of initiation factors and AUG codon; (b) this binding occurred over a wide Mg2+ concentration range; significant binding was observed even at 20 mM Mg2+; (c) upon addition of 60 S ribosomes, a significant part of Met-tRNAfMet bound to 40 S ribosomes was transferred to 80 S complex. This transfer reaction had a sharp Mg2+ optimum around 2 mM. Met-tRNAfMet-80 S-AUG complex thus formed was active in Met-puromycin synthesis; (d) Met-tRNAfMet deacylase present in crude 0.5 M KCl ribosomal wash is a potent inhibitor of the binding reaction as it deacylates Met-tRNAfMet in the Met-tRNAfMet-40 S-AUG complex; (e) glutaraldehyde (0.5%) degrades Met-tRNAfMet-40 S-AUG complex but increases the background binding of Met-tRNAfMet to 40 S ribosomes in the absence of AUG codon; (f) polynucleotides containing uracil and adenosine are strong inhibitors of Met-tRNAfMet binding to 40 S ribosomes. The order of inhibitory activities of the polynucleotides tested was as follows: poly(rU)-poly(rA) (2:1) greater than poly(rU)-poly(rA) (1:1) greater than poly(rU) greater than poly(rA). Other RNAs tested such as poly(rC), poly(rI)-poly(rC) and phi6 bacteriophage RNA (double-stranded) were without significant effects on the Met-tRNAfMet-binding reaction.", "PMID": 977577}
{"id": "pubmed23n0032_11175", "title": "Lipid-saccharide intermediates in glycoprotein biosynthesis. I. Formation of an oligosaccharide-lipid by thyroid slices and evaluation of its role in protein glycosylation.", "content": "Thyroid slices were found to incorporate radioactivity from 14C-labeled sugars into the carbohydrate moiety of a polar lipid soluble in chloroform/methanol/water, 10/10/3. This radiolabeled glycolipid was purified by chromatography on DEAE-cellulose and was shown to have as its monosaccharide constituents mannose, glucose, and glucosamine. This compound cloud also be labeled by incubation of thyroid slices with [3H]mevalonic acid or [32P]phosphate as demonstrated by the coincidence of elution profiles upon DEAE-cellulose chromatography. This suggested that the lipid portion of the molecule is a polyprenol derivative and the lipid-saccharide linkage region involves a phosphate bridge. Mild acid hydrolysis of the glycolipid labeled with 14C in its carbohydrate released a neutral oligosaccharide which on the basis of Bio-Gel filtration studies was shown to have a molecular weight of approximately 2,400. This oligosaccharide contained [14C]mannose and [14C]glucose in about the same ratio as that occurring in the intact glycolipid. The oligosaccharide-lipid appeared to be distributed rather widely in thyroid particulate fractions obtained after differential and density gradient centrifugation. Its highest specific activity occurred in fractions rich in endoplasmic reticulum. By means of pulse-chase experiments in slices a relationship was demonstrated between the disappearance of radioactivity from the lipid-bound oligosaccharide and its appearance in protein-bound form. When protein synthesis was inhibited by the addition of puromycin during the chase period of the experiment transfer of oligosaccharide from the lipid to protein appeared to be blocked and the level of radiolabeled oligosaccharide-lipid increased. The observation that mannose and glucose were similarly affected during the pulse-chase studies suggests that transfer of the intact oligosaccharide unit was involved in the addition of carbohydrate to protein.", "contents": "Lipid-saccharide intermediates in glycoprotein biosynthesis. I. Formation of an oligosaccharide-lipid by thyroid slices and evaluation of its role in protein glycosylation. Thyroid slices were found to incorporate radioactivity from 14C-labeled sugars into the carbohydrate moiety of a polar lipid soluble in chloroform/methanol/water, 10/10/3. This radiolabeled glycolipid was purified by chromatography on DEAE-cellulose and was shown to have as its monosaccharide constituents mannose, glucose, and glucosamine. This compound cloud also be labeled by incubation of thyroid slices with [3H]mevalonic acid or [32P]phosphate as demonstrated by the coincidence of elution profiles upon DEAE-cellulose chromatography. This suggested that the lipid portion of the molecule is a polyprenol derivative and the lipid-saccharide linkage region involves a phosphate bridge. Mild acid hydrolysis of the glycolipid labeled with 14C in its carbohydrate released a neutral oligosaccharide which on the basis of Bio-Gel filtration studies was shown to have a molecular weight of approximately 2,400. This oligosaccharide contained [14C]mannose and [14C]glucose in about the same ratio as that occurring in the intact glycolipid. The oligosaccharide-lipid appeared to be distributed rather widely in thyroid particulate fractions obtained after differential and density gradient centrifugation. Its highest specific activity occurred in fractions rich in endoplasmic reticulum. By means of pulse-chase experiments in slices a relationship was demonstrated between the disappearance of radioactivity from the lipid-bound oligosaccharide and its appearance in protein-bound form. When protein synthesis was inhibited by the addition of puromycin during the chase period of the experiment transfer of oligosaccharide from the lipid to protein appeared to be blocked and the level of radiolabeled oligosaccharide-lipid increased. The observation that mannose and glucose were similarly affected during the pulse-chase studies suggests that transfer of the intact oligosaccharide unit was involved in the addition of carbohydrate to protein.", "PMID": 977578}
{"id": "pubmed23n0032_11176", "title": "Lipid-saccharide intermediates in glycoprotein biosynthesis. II. Studies on the structure of an oligosaccharide-lipid from thyroid.", "content": "Structural studies have been performed on an oligosaccharide-lipid from thyroid believed to be an intermediate in glycoprotein synthesis. For these investigations the compound was isolated from the gland in unlabeled form as well as differentially radiolabeled in its saccharide, lipid, and phosphate portions by incubation of slices with [14C]- or [3H]glucose, [3H]mevalonic acid and [32P]phosphate, respectively. The unlabeled oligosaccharide-lipid was obtained in a chloroform/methanol/water (10/10/3) extract in a yield of about 1 nmol/g of thyroid and was purified therefrom by DEAE-cellulose chromatography. The saccharide moiety released from the glycolipid by mild acid hydrolysis was isolated by gel filtration and contained 11 mannose, 1 to 2 glucose, and 2 N-acetylglucosamine residues. The reducing terminal position of the oligosaccharide was occupied by 1 of the glucosamine residues and from these analyses a molecular weight of 2,415 was calculated. That glucose is an integral part of the molecule was further demonstrated by the finding that during Dowex 50 chromatography it remained as a constituent of the positively charged oligosaccharide produced by deacetylation with alkaline borohydride at 80 degrees. The phosphorus content of the purified unlabeled oligosaccharide-lipid was determined to be 2 residues per molecule, suggesting the presence of a pyrophosphate bridge between its carbohydrate and lipid portions. Further evidence for such a linkage region was provided by characterization of the products from mild acid and alkaline hydrolysis of the differentially radiolabeled glycolipid. These included dolichyl mono- and pyrophosphate, oligosaccharide phosphate, and free oligosaccharide. Digestion with alpha-mannosidase of the radiolabeled glycolipid led to the release of 39% of its mannose while from the free oligosaccharide 53% of this sugar was removed. Acetolysis of the [14C]oligosaccharide yielded a mannobiose and mannotriose as well as larger fragments consisting of mannose, glucose, and glucosamine. Smith periodate degradation gave rise to a small core segment (6 glycose residues) made up only of mannose and glucosamine from which half of the mannose residues could be released by alpha-mannosidase digestion. From these studies a tentative structure for the carbohydrate moiety of the oligosaccharide-lipid has been proposed. In this formulation an inner core (periodate-resistant) made up of 4 mannose and 2 N-acetylglucosamine residues is attached to the pyrophosphate group by the most internal glucosamine. This core, as well as an additional mannose and 1 to 2 glucose residues, constitutes the alpha-mannosidase-resistant fragment. More peripherally are found other mannose residues, all in alpha-linkage. In this structural scheme the glucose is located so as to prevent the enzymatic release of more internally situated alpha-linked mannose residues.", "contents": "Lipid-saccharide intermediates in glycoprotein biosynthesis. II. Studies on the structure of an oligosaccharide-lipid from thyroid. Structural studies have been performed on an oligosaccharide-lipid from thyroid believed to be an intermediate in glycoprotein synthesis. For these investigations the compound was isolated from the gland in unlabeled form as well as differentially radiolabeled in its saccharide, lipid, and phosphate portions by incubation of slices with [14C]- or [3H]glucose, [3H]mevalonic acid and [32P]phosphate, respectively. The unlabeled oligosaccharide-lipid was obtained in a chloroform/methanol/water (10/10/3) extract in a yield of about 1 nmol/g of thyroid and was purified therefrom by DEAE-cellulose chromatography. The saccharide moiety released from the glycolipid by mild acid hydrolysis was isolated by gel filtration and contained 11 mannose, 1 to 2 glucose, and 2 N-acetylglucosamine residues. The reducing terminal position of the oligosaccharide was occupied by 1 of the glucosamine residues and from these analyses a molecular weight of 2,415 was calculated. That glucose is an integral part of the molecule was further demonstrated by the finding that during Dowex 50 chromatography it remained as a constituent of the positively charged oligosaccharide produced by deacetylation with alkaline borohydride at 80 degrees. The phosphorus content of the purified unlabeled oligosaccharide-lipid was determined to be 2 residues per molecule, suggesting the presence of a pyrophosphate bridge between its carbohydrate and lipid portions. Further evidence for such a linkage region was provided by characterization of the products from mild acid and alkaline hydrolysis of the differentially radiolabeled glycolipid. These included dolichyl mono- and pyrophosphate, oligosaccharide phosphate, and free oligosaccharide. Digestion with alpha-mannosidase of the radiolabeled glycolipid led to the release of 39% of its mannose while from the free oligosaccharide 53% of this sugar was removed. Acetolysis of the [14C]oligosaccharide yielded a mannobiose and mannotriose as well as larger fragments consisting of mannose, glucose, and glucosamine. Smith periodate degradation gave rise to a small core segment (6 glycose residues) made up only of mannose and glucosamine from which half of the mannose residues could be released by alpha-mannosidase digestion. From these studies a tentative structure for the carbohydrate moiety of the oligosaccharide-lipid has been proposed. In this formulation an inner core (periodate-resistant) made up of 4 mannose and 2 N-acetylglucosamine residues is attached to the pyrophosphate group by the most internal glucosamine. This core, as well as an additional mannose and 1 to 2 glucose residues, constitutes the alpha-mannosidase-resistant fragment. More peripherally are found other mannose residues, all in alpha-linkage. In this structural scheme the glucose is located so as to prevent the enzymatic release of more internally situated alpha-linked mannose residues.", "PMID": 977579}
{"id": "pubmed23n0032_11177", "title": "Lipid-saccharide intermediates in glycoprotein biosynthesis. III. Comparison of oligosaccharide-lipids formed by slices from several tissues.", "content": "The synthesis of oligosaccharide-lipids thought to play a role in the attachment of carbohydrate to protein has been studied in incubations of slices from calf kidney, pancreas, thymus, and liver, as well as from hen oviduct. These compounds were characterized after radiolabeling of their saccharide moiety by incubation with [14C]glucose or [14C]mannose and a comparison was made with the oligosaccharide-lipid produced by thyroid slices. Furthermore, the unlabeled glycolipid was prepared from hen oviduct for the purpose of quantitating its sugar constituents. Purification of the oligosaccharide-lipids extracted with chloroform/methanol/water (10/10/3) was achieved by DEAE-cellulose chromatography and their carbohydrate moieties were released by mild acid hydrolysis. On the basis of gel filtration it was determined that the lipid-bound oligosaccharides formed by oviduct, thymus, kidney, and liver had molecular weights comparable to that from thyroid (about 2400). The saccharide moiety of the glycolipid from pancreas was however distinctly smaller in size with a molecular weight of approximately 1800. Analyses of the radiolabeled oligosaccharide-lipids from oviduct, kidney, and thymus indicated that they, like the compound from thyroid slices, but unlike those believed to be formed by cell-free systems from various tissues, contained glucose in addition to mannose and N-acetylglucosamine as their monosaccharide constituents. This compositional data was supported by the finding that the unlabeled oligosaccharide from oviduct consists of 10 mannose, 1 glucose, and 2 N-acetylglucosamine residues. Sodium borohydride reduction of this oviduct saccharide moiety indicated that 1 of the 2 glucosamines was situated in a reducing terminal position. The radiolabeled oligosaccharide from the glycolipid produced by pancreas differed from the others analyzed in that it contained only trace amounts of glucose. Upon treatment with alpha-mannosidase this glucose-deficient pancreatic oligosaccharide was extensively digested (85% of the mannose released). In contrast, the carbohydrate moieties of oviduct, kidney, and thymus, like that of thyroid, underwent a more limited digestion with the alpha-mannosidase (55% or less of the mannose released) suggesting that the presence of glucose may serve to block a more complete degradation of these oligosaccharides by this enzyme.", "contents": "Lipid-saccharide intermediates in glycoprotein biosynthesis. III. Comparison of oligosaccharide-lipids formed by slices from several tissues. The synthesis of oligosaccharide-lipids thought to play a role in the attachment of carbohydrate to protein has been studied in incubations of slices from calf kidney, pancreas, thymus, and liver, as well as from hen oviduct. These compounds were characterized after radiolabeling of their saccharide moiety by incubation with [14C]glucose or [14C]mannose and a comparison was made with the oligosaccharide-lipid produced by thyroid slices. Furthermore, the unlabeled glycolipid was prepared from hen oviduct for the purpose of quantitating its sugar constituents. Purification of the oligosaccharide-lipids extracted with chloroform/methanol/water (10/10/3) was achieved by DEAE-cellulose chromatography and their carbohydrate moieties were released by mild acid hydrolysis. On the basis of gel filtration it was determined that the lipid-bound oligosaccharides formed by oviduct, thymus, kidney, and liver had molecular weights comparable to that from thyroid (about 2400). The saccharide moiety of the glycolipid from pancreas was however distinctly smaller in size with a molecular weight of approximately 1800. Analyses of the radiolabeled oligosaccharide-lipids from oviduct, kidney, and thymus indicated that they, like the compound from thyroid slices, but unlike those believed to be formed by cell-free systems from various tissues, contained glucose in addition to mannose and N-acetylglucosamine as their monosaccharide constituents. This compositional data was supported by the finding that the unlabeled oligosaccharide from oviduct consists of 10 mannose, 1 glucose, and 2 N-acetylglucosamine residues. Sodium borohydride reduction of this oviduct saccharide moiety indicated that 1 of the 2 glucosamines was situated in a reducing terminal position. The radiolabeled oligosaccharide from the glycolipid produced by pancreas differed from the others analyzed in that it contained only trace amounts of glucose. Upon treatment with alpha-mannosidase this glucose-deficient pancreatic oligosaccharide was extensively digested (85% of the mannose released). In contrast, the carbohydrate moieties of oviduct, kidney, and thymus, like that of thyroid, underwent a more limited digestion with the alpha-mannosidase (55% or less of the mannose released) suggesting that the presence of glucose may serve to block a more complete degradation of these oligosaccharides by this enzyme.", "PMID": 977580}
{"id": "pubmed23n0032_11178", "title": "The NH2-terminal region of the beta chain of sickle hemoglobin. I. Synthesis and purification of oligopeptides.", "content": "Five peptides from the NH2-terminal region of the beta chain of hemoglobin S, betaS (1-13), betaS (3-13), betaS (1-8), betaS (4-10), And betaS (4-8), have been synthesized by a rapid solid phase method based on the Merrifield procedure. In addition, one peptide, betaS (3-13), has also been synthesized by the original Merrifield method. We have shown that the products of the two methods are comparable, that gel filtration is a useful method for removing truncated fragments of the desired oligopeptide, and that measurement of the efficiency of coupling at each step is an important adjunct to amino acid analysis in determining purity. Peptides of the purity achieved by these methods may be used to fractionate antibodies to the native hemoglobin S, in the characterization of antigen-binding properties of specific antibodies, and in other studies of peptide-protein interactions.", "contents": "The NH2-terminal region of the beta chain of sickle hemoglobin. I. Synthesis and purification of oligopeptides. Five peptides from the NH2-terminal region of the beta chain of hemoglobin S, betaS (1-13), betaS (3-13), betaS (1-8), betaS (4-10), And betaS (4-8), have been synthesized by a rapid solid phase method based on the Merrifield procedure. In addition, one peptide, betaS (3-13), has also been synthesized by the original Merrifield method. We have shown that the products of the two methods are comparable, that gel filtration is a useful method for removing truncated fragments of the desired oligopeptide, and that measurement of the efficiency of coupling at each step is an important adjunct to amino acid analysis in determining purity. Peptides of the purity achieved by these methods may be used to fractionate antibodies to the native hemoglobin S, in the characterization of antigen-binding properties of specific antibodies, and in other studies of peptide-protein interactions.", "PMID": 977581}
{"id": "pubmed23n0032_11179", "title": "The NH2-terminal region of the sickle hemoglobin beta chain. II. Characterization of monospecific antibodies.", "content": "We have previously shown that antibodies specific for hemoglobin S could be fractionated by absorption of an antiserum to hemoglobin S to Sepharose containing a synthetic oligopeptide. betaS (1-13), corresponding to the first 13 amino acid residues of the beta chain of hemoglobin S. We report here that this antibody population, anti-betaS (1-13), shows considerable restriction of heterogeneity in isoelectric focusing studies and monospecificity on velocity ultracentrifugation in the presence of hemoglobin S. The binding of various hemoglobin species to anti-betaS (1-13) was studied using a double antibody radioimmunoassay with [14C]carbamoylated hemoglobin S. Carbonmonoxy-, oxy-, met-, and cyanmethemoglobin S reacted equally with the antibody, but deoxyhemoglobin (with or without organic phosphates) reacted differently. Hemoglobin A and several hemoglobin mutants with alterations in the NH2-terminal region of the beta chain did not displace labeled hemoglobin S from anti-betaS (1-13). BETAS chains reacted with the antibody, but less well than hemoglobin S, while betaA and alpha chains, and globins did not react with the antibody. The synthetic peptide, betaS (1-13), used for fractionation, reacted with the antibody about 300-fold less efficiently than hemoglobin S. BetaS (3-13) was even less reactive, while smaller peptides which included the valine residue at position 6 displaced little of the tracer [14C]carbamoylated hemoglobin S at concentrations as high as 10(-2) M. We interpret these results to indicate that this method of immunoabsorption has produced a monospecific subfraction of antibodies which is specific for the NH2-terminal region of the beta chain of hemoglobin S in its native conformation.", "contents": "The NH2-terminal region of the sickle hemoglobin beta chain. II. Characterization of monospecific antibodies. We have previously shown that antibodies specific for hemoglobin S could be fractionated by absorption of an antiserum to hemoglobin S to Sepharose containing a synthetic oligopeptide. betaS (1-13), corresponding to the first 13 amino acid residues of the beta chain of hemoglobin S. We report here that this antibody population, anti-betaS (1-13), shows considerable restriction of heterogeneity in isoelectric focusing studies and monospecificity on velocity ultracentrifugation in the presence of hemoglobin S. The binding of various hemoglobin species to anti-betaS (1-13) was studied using a double antibody radioimmunoassay with [14C]carbamoylated hemoglobin S. Carbonmonoxy-, oxy-, met-, and cyanmethemoglobin S reacted equally with the antibody, but deoxyhemoglobin (with or without organic phosphates) reacted differently. Hemoglobin A and several hemoglobin mutants with alterations in the NH2-terminal region of the beta chain did not displace labeled hemoglobin S from anti-betaS (1-13). BETAS chains reacted with the antibody, but less well than hemoglobin S, while betaA and alpha chains, and globins did not react with the antibody. The synthetic peptide, betaS (1-13), used for fractionation, reacted with the antibody about 300-fold less efficiently than hemoglobin S. BetaS (3-13) was even less reactive, while smaller peptides which included the valine residue at position 6 displaced little of the tracer [14C]carbamoylated hemoglobin S at concentrations as high as 10(-2) M. We interpret these results to indicate that this method of immunoabsorption has produced a monospecific subfraction of antibodies which is specific for the NH2-terminal region of the beta chain of hemoglobin S in its native conformation.", "PMID": 977582}
{"id": "pubmed23n0032_11180", "title": "Proteoglycans from bovine proximal humeral articular cartilage. Structural basis for the polydispersity of proteoglycan subunit.", "content": "Polydisperse proteoglycan subunit from bovine proximal humeral articular cartilage has been separated into a series of relatively monodisperse fractions which have been chemically and physically characterized. The proteoglycan subunit species of the lowest molecular weight contains the least chondroitin sulfate and had an amino acid composition relatively low in serine and glycine and relatively high in cysteine, methionine, and aspartic acid, almost identical to that of the hyaluronic acid-binding region of proteoglycan subunit isolated by Heinegard and Hascall (Heinegard, D., and Hascall, V.C. (1974) J. Biol. Chem. 249, 4250-4256). The molecular weight of proteoglycan subunit increases in proportion to its chondroitin sulfate content. As the molecular weight and chondroitin sulfate content of proteoglycan subunit increase, there is a parallel increase in the serine and glycine contents, and a decrease in the cysteine, methionine, and aspartic acid contents of proteoglycan subunit core protein. The pattern of polydispersity observed strongly supports the concept that proteoglycan subunit core protein contains a hyaluronic acid-binding region of constant size and composition and a polysaccharide attachment region of variable length and composition, composed of repeating peptide sequences containing serine and glycine in equimolar amounts.", "contents": "Proteoglycans from bovine proximal humeral articular cartilage. Structural basis for the polydispersity of proteoglycan subunit. Polydisperse proteoglycan subunit from bovine proximal humeral articular cartilage has been separated into a series of relatively monodisperse fractions which have been chemically and physically characterized. The proteoglycan subunit species of the lowest molecular weight contains the least chondroitin sulfate and had an amino acid composition relatively low in serine and glycine and relatively high in cysteine, methionine, and aspartic acid, almost identical to that of the hyaluronic acid-binding region of proteoglycan subunit isolated by Heinegard and Hascall (Heinegard, D., and Hascall, V.C. (1974) J. Biol. Chem. 249, 4250-4256). The molecular weight of proteoglycan subunit increases in proportion to its chondroitin sulfate content. As the molecular weight and chondroitin sulfate content of proteoglycan subunit increase, there is a parallel increase in the serine and glycine contents, and a decrease in the cysteine, methionine, and aspartic acid contents of proteoglycan subunit core protein. The pattern of polydispersity observed strongly supports the concept that proteoglycan subunit core protein contains a hyaluronic acid-binding region of constant size and composition and a polysaccharide attachment region of variable length and composition, composed of repeating peptide sequences containing serine and glycine in equimolar amounts.", "PMID": 977583}
{"id": "pubmed23n0032_11181", "title": "The anomeric configuration of the D-mannosyl retinyl phosphate formed in rat liver microsomes.", "content": "Alkaline hydrolysis at 65 degrees converted D-mannosyl retinyl phosphate primarily into D-mannose 2-phosphate which was identified by its behavior on paper chromatography in two solvent systems, as well as by its resistance to acid hydrolysis under conditions that converted authentic alpha-D-mannosyl phosphate to mannose. When the D-mannosyl retinyl phosphate was hydrolyzed in alkali at 100 degrees, the main product was beta-D-mannosyl phosphate and not the alpha anomer (as shown by chromatography with authentic samples on thin layers of silica gel). No evidence for enzymatic hydrolysis of D-mannosyl retinyl phosphate by alpha-mannosidase was obtainable. It is concluded that the compound under investigation is beta-D-mannosyl retinyl phosphate.", "contents": "The anomeric configuration of the D-mannosyl retinyl phosphate formed in rat liver microsomes. Alkaline hydrolysis at 65 degrees converted D-mannosyl retinyl phosphate primarily into D-mannose 2-phosphate which was identified by its behavior on paper chromatography in two solvent systems, as well as by its resistance to acid hydrolysis under conditions that converted authentic alpha-D-mannosyl phosphate to mannose. When the D-mannosyl retinyl phosphate was hydrolyzed in alkali at 100 degrees, the main product was beta-D-mannosyl phosphate and not the alpha anomer (as shown by chromatography with authentic samples on thin layers of silica gel). No evidence for enzymatic hydrolysis of D-mannosyl retinyl phosphate by alpha-mannosidase was obtainable. It is concluded that the compound under investigation is beta-D-mannosyl retinyl phosphate.", "PMID": 977584}
{"id": "pubmed23n0032_11182", "title": "Effects of estrogen on gene expression in the chick oviduct. Control of ovalbumin gene expression by non-histone proteins.", "content": "Chromatin was isolated from the oviducts of chicks which had either received diethylstilbestrol stimulation for 14 days (stimulated chromatin) or been treated and then withdrawn from hormone administration (withdrawn chromatin). These chromatin preparations were dissociated and then reconstituted in the presence of various non-histone protein fractions. cDNAov was used as a probe for measuring the level of mRNAov sequences synthesized in vitro from various reconstituted chromatins. Our results indicate that extractable non-histone proteins from stimulated chromatin are capable of activating the in vitro transcription of the ovalbumin gene when included in the reconstitution of withdrawn chromatin. On the other hand, addition of extractable non-histone proteins from withdrawn chromatin to stimulated chromatin does not affect the synthesis of mRNAov sequences. Therefore, the extractable non-histone proteins from stimulated chromatin contain a positive regulator(s) which controls the in vitro expression of the ovalbumin gene.", "contents": "Effects of estrogen on gene expression in the chick oviduct. Control of ovalbumin gene expression by non-histone proteins. Chromatin was isolated from the oviducts of chicks which had either received diethylstilbestrol stimulation for 14 days (stimulated chromatin) or been treated and then withdrawn from hormone administration (withdrawn chromatin). These chromatin preparations were dissociated and then reconstituted in the presence of various non-histone protein fractions. cDNAov was used as a probe for measuring the level of mRNAov sequences synthesized in vitro from various reconstituted chromatins. Our results indicate that extractable non-histone proteins from stimulated chromatin are capable of activating the in vitro transcription of the ovalbumin gene when included in the reconstitution of withdrawn chromatin. On the other hand, addition of extractable non-histone proteins from withdrawn chromatin to stimulated chromatin does not affect the synthesis of mRNAov sequences. Therefore, the extractable non-histone proteins from stimulated chromatin contain a positive regulator(s) which controls the in vitro expression of the ovalbumin gene.", "PMID": 977585}
{"id": "pubmed23n0032_11183", "title": "Purification and properties of an endo-beta-N-acetylglucosaminidase from hen oviduct.", "content": "An endo-beta-N-acetylglucosaminidase has been purified extensively from hen oviduct extracts and found to have a somewhat broader substrate specificity than the previously reported endoglycosidases from Dipolcoccus pneumoniae and Streptomyces plicatus (Arakawa, M., and Muramatsu, T. (1974) J. Biochem. (Tokyo) 76, 307-317; Tarentino, A. L., and Maley, F. (1975) Biochem. Biophys. Res. Commun. 67, 455-462. The enzyme was shown to hydrolyze and di-N-acetylchitobiosyl bond in such compounds as Asn(GlcNAc)2(Man)3, Asn(GlcNAc)2(Man)5, Asn(GlcNAc)2(Man)6 and a glycopeptide from immunoglobulin M, (aa)x-Asn(GlcNAc)2(Man)3(Fuc)1. The capacity to hydrolyze the latter compound is a characteristic of the endoglycosidase from D. pneumoniae, but not that from S. plicatus. Competitive inhibition studies indicate that a single enzyme in the hen oviduct endoglycosidase preparation is probably responsible for hydrolyzing both fucose-containing the fucose-depleted substrates. The existence of an endo-beta-N-acetylglucosaminidase in animal tissue may explain why oligosaccharides with N-acetylglucosamine on the reducing terminus accumulate in certain lysosomal storage diseases.", "contents": "Purification and properties of an endo-beta-N-acetylglucosaminidase from hen oviduct. An endo-beta-N-acetylglucosaminidase has been purified extensively from hen oviduct extracts and found to have a somewhat broader substrate specificity than the previously reported endoglycosidases from Dipolcoccus pneumoniae and Streptomyces plicatus (Arakawa, M., and Muramatsu, T. (1974) J. Biochem. (Tokyo) 76, 307-317; Tarentino, A. L., and Maley, F. (1975) Biochem. Biophys. Res. Commun. 67, 455-462. The enzyme was shown to hydrolyze and di-N-acetylchitobiosyl bond in such compounds as Asn(GlcNAc)2(Man)3, Asn(GlcNAc)2(Man)5, Asn(GlcNAc)2(Man)6 and a glycopeptide from immunoglobulin M, (aa)x-Asn(GlcNAc)2(Man)3(Fuc)1. The capacity to hydrolyze the latter compound is a characteristic of the endoglycosidase from D. pneumoniae, but not that from S. plicatus. Competitive inhibition studies indicate that a single enzyme in the hen oviduct endoglycosidase preparation is probably responsible for hydrolyzing both fucose-containing the fucose-depleted substrates. The existence of an endo-beta-N-acetylglucosaminidase in animal tissue may explain why oligosaccharides with N-acetylglucosamine on the reducing terminus accumulate in certain lysosomal storage diseases.", "PMID": 977586}
{"id": "pubmed23n0032_11184", "title": "Formation of glutathione conjugates of prostaglandin A1 in human red blood cells.", "content": "When prostaglandin A1 was incubated with a Tris/saline suspension of washed human red blood cells, a substantial amount was converted to polar metabolites. These were purified by solvent extraction, XAD-2 column, and cellulose thin layer chromatography and characterized by chromatography, amino acid analysis, and mass spectrometry. The polar metabolites were a mixture of two glutathione conjugates of prostaglandin A1. The first (approximately 40%) was identical with the product of the nonenzymic reaction of glutathione with prostaglandin A1. The second (approximately 60%) was formed from the first by reduction of the 9-keto group of the prostaglandin moiety. The latter compound was also prepared synthetically by treating the glutathione conjugate of prostaglandin A1 with sodium borohydride.", "contents": "Formation of glutathione conjugates of prostaglandin A1 in human red blood cells. When prostaglandin A1 was incubated with a Tris/saline suspension of washed human red blood cells, a substantial amount was converted to polar metabolites. These were purified by solvent extraction, XAD-2 column, and cellulose thin layer chromatography and characterized by chromatography, amino acid analysis, and mass spectrometry. The polar metabolites were a mixture of two glutathione conjugates of prostaglandin A1. The first (approximately 40%) was identical with the product of the nonenzymic reaction of glutathione with prostaglandin A1. The second (approximately 60%) was formed from the first by reduction of the 9-keto group of the prostaglandin moiety. The latter compound was also prepared synthetically by treating the glutathione conjugate of prostaglandin A1 with sodium borohydride.", "PMID": 977587}
{"id": "pubmed23n0032_11185", "title": "Purification and properties of filamin, and actin binding protein from chicken gizzard.", "content": "Filamin, a protein recently identified in chicken gizzard (Wang, K., Ash, F., and Singer, S. J. (1975) Proc. Natl. Acad. Sci. U. S. A. 72, 4483-4486), has been purified free of other components and its molecular properties have been examined. Filamin has a sedimentation constant (S020,w) of 8.86 S and a partial specific volume of 0.734 ml/g. Sedimentation equilibrium experiments give a value of 498,000 for the molecular weight of native filamin. From these data a frictional ratio of 2.32 has been calculated. On sodium dodecyl sulfate gel electrophoresis, filamin migrates as a single protein band with an estimated molecular weight of 240,000. Filamin is a soluble protein and under a variety of conditions tested does not by itself form filaments or precipitate from solution. However, filamin binds to rabbit skeletal muscle F-actin, and the complex is readily sedimented by centrifugation to yield a gelatinous pellet containing actin and filamin. These results indicate that filamin is a dimeric protein with a moderate degree of asymmetry that binds to actin. The results also suggest that the distribution of filamin in cells is derived from its interaction with polymerized actin.", "contents": "Purification and properties of filamin, and actin binding protein from chicken gizzard. Filamin, a protein recently identified in chicken gizzard (Wang, K., Ash, F., and Singer, S. J. (1975) Proc. Natl. Acad. Sci. U. S. A. 72, 4483-4486), has been purified free of other components and its molecular properties have been examined. Filamin has a sedimentation constant (S020,w) of 8.86 S and a partial specific volume of 0.734 ml/g. Sedimentation equilibrium experiments give a value of 498,000 for the molecular weight of native filamin. From these data a frictional ratio of 2.32 has been calculated. On sodium dodecyl sulfate gel electrophoresis, filamin migrates as a single protein band with an estimated molecular weight of 240,000. Filamin is a soluble protein and under a variety of conditions tested does not by itself form filaments or precipitate from solution. However, filamin binds to rabbit skeletal muscle F-actin, and the complex is readily sedimented by centrifugation to yield a gelatinous pellet containing actin and filamin. These results indicate that filamin is a dimeric protein with a moderate degree of asymmetry that binds to actin. The results also suggest that the distribution of filamin in cells is derived from its interaction with polymerized actin.", "PMID": 977588}
{"id": "pubmed23n0032_11186", "title": "Uptake of hydroxocobalamin by rat liver mitochondria. Binding to a mitochondrial protein.", "content": "Lysosome-free preparations of rat liver mitochondria take up hydroxo[57Co]cobalamin by a process which is dependent on mitochondrial swelling, rather than on energy or ion fluxes. The uptake system is saturable and unidirectional, leading to inside/outside concentration ratios of 17. The process also exhibits specificity: cyano[57Co]cobalamin is taken up less rapidly and to a lesser extent than hydroxocobalamin; methylcobalamin and adenoslcobalamin inhibit hydroxocobalamin uptake markedly, while cyanocobalamin does not. The [57Co]cobalamin ([57Co]Cbl) taken up is bound to a mitochondrial protein whose apparent molecular weight is 120,000 by Sephadex G-150 chromatography. Double reciprocal plots of bound [57Co]Cbl versus medium [57Co]Cbl concentration yield estimates for bound Cblmax of 29 pmol/mg of protein and for Kd is 8.2 muM. We conclude that mitochondrial uptake of cobalamins occurs via the diffusion of free cobalamins into the mitochondria and their subsequent binding to a high affinity mitochondrial protein(s) which we propose to be the source of the unidirectional character, the saturability, and the specificity of the uptake system.", "contents": "Uptake of hydroxocobalamin by rat liver mitochondria. Binding to a mitochondrial protein. Lysosome-free preparations of rat liver mitochondria take up hydroxo[57Co]cobalamin by a process which is dependent on mitochondrial swelling, rather than on energy or ion fluxes. The uptake system is saturable and unidirectional, leading to inside/outside concentration ratios of 17. The process also exhibits specificity: cyano[57Co]cobalamin is taken up less rapidly and to a lesser extent than hydroxocobalamin; methylcobalamin and adenoslcobalamin inhibit hydroxocobalamin uptake markedly, while cyanocobalamin does not. The [57Co]cobalamin ([57Co]Cbl) taken up is bound to a mitochondrial protein whose apparent molecular weight is 120,000 by Sephadex G-150 chromatography. Double reciprocal plots of bound [57Co]Cbl versus medium [57Co]Cbl concentration yield estimates for bound Cblmax of 29 pmol/mg of protein and for Kd is 8.2 muM. We conclude that mitochondrial uptake of cobalamins occurs via the diffusion of free cobalamins into the mitochondria and their subsequent binding to a high affinity mitochondrial protein(s) which we propose to be the source of the unidirectional character, the saturability, and the specificity of the uptake system.", "PMID": 977589}
{"id": "pubmed23n0032_11187", "title": "Kinetics of active center formation in dog plasminogin by streptokinase and activity of a modified streptokinase.", "content": "The rate of activation of dog plasminogen by excess streptokinase showed a significant delay as compared to the rate of activation with catalytic amounts of streptokinase. Studies of the reaction at high streptokinase levels with the active center reagent, p-nitrophenyl-p-guanidinobenzoate showed that only a fraction (13%) of the potential active centers were developed in a equimolar mixture of streptokinase and dog plasminogen in 15s and more than 10 min were required for the formation of 1 mol of active sites. In the first 15s, the yield of active sites could not be increased by increasing streptokinase 10-fold over the molar concentration of plasminogen, suggesting that active center development rather than complex formation was the rate-limiting step. The delayed reactivity seen in this system provides an interesting model for the study of conformationally induced active center formation. With catalytic amounts of streptokinase, the activation proceeded rapidly but reached a plateau, indicating the loss of activator activity in the reaction mixture. With successive additions of fresh streptokinase, complete activation was achieved. Polyacrylamide gel electorphoresis showed that a stable streptokinase-plasmin complex formed. However, in contrast to the human plasmin-steptokinase complex, a potent plasminogen activator in which streptokinase is found as a residue of 37,000 daltons, dog plasmin-streptokinase complex contained a residue of 25,700 daltons and the complex was inactive against canine and human plasminogen. The 25,700 fragment along, however, showed considerable activator activity when tested with human and dog plasminogens.", "contents": "Kinetics of active center formation in dog plasminogin by streptokinase and activity of a modified streptokinase. The rate of activation of dog plasminogen by excess streptokinase showed a significant delay as compared to the rate of activation with catalytic amounts of streptokinase. Studies of the reaction at high streptokinase levels with the active center reagent, p-nitrophenyl-p-guanidinobenzoate showed that only a fraction (13%) of the potential active centers were developed in a equimolar mixture of streptokinase and dog plasminogen in 15s and more than 10 min were required for the formation of 1 mol of active sites. In the first 15s, the yield of active sites could not be increased by increasing streptokinase 10-fold over the molar concentration of plasminogen, suggesting that active center development rather than complex formation was the rate-limiting step. The delayed reactivity seen in this system provides an interesting model for the study of conformationally induced active center formation. With catalytic amounts of streptokinase, the activation proceeded rapidly but reached a plateau, indicating the loss of activator activity in the reaction mixture. With successive additions of fresh streptokinase, complete activation was achieved. Polyacrylamide gel electorphoresis showed that a stable streptokinase-plasmin complex formed. However, in contrast to the human plasmin-steptokinase complex, a potent plasminogen activator in which streptokinase is found as a residue of 37,000 daltons, dog plasmin-streptokinase complex contained a residue of 25,700 daltons and the complex was inactive against canine and human plasminogen. The 25,700 fragment along, however, showed considerable activator activity when tested with human and dog plasminogens.", "PMID": 977590}
{"id": "pubmed23n0032_11188", "title": "Purification of a soluble rat liver protein that stimulates microsomal 4-methyl sterol oxidase activity.", "content": "A soluble rat liver protein that stimulates microsomal methyl sterol oxidase activity has been isolated and purified to homogeneity by salt fractionation, differential heat inactivation, two calcium phosphate gel association, and Sephadex filtration. The protein, as isolated, may be dissociated with detergent into subunits with a molecular weight of approximately 10,300, as determined electrophoretically. In addition to this stimulatory protein, postmicrosomal supernatant fraction of rat liver contains a low molecular weight methyl sterol oxidase inhibitor, possibly cholesterol ester, which is removed during protein purification. Purified soluble protein enhances the observed rate of oxidative attack of the methyl sterol substrates, 4, 4-dimethyl-5alpha-cholest-7-en-3beta-ol and 4alpha-methyl-5alpha-cholest-7-en-3beta-ol. Addition of increasing amounts of either partially purified or homogeneous soluble protein yields hyperbolic stimulation, from which a K'm of about 7 muM has been calculated (based on a monomeric molecular weight of 10,300). Sequential additions of the soluble protein yield equal increments of stimulation. These results are consistent with the suggestion that the soluble protein may be a reactant in the oxidative process. Methyl sterol oxidase is inhitited in vitro by cholesterol, several oxygenated sterols, and cholesterol esters. The extent of inhibition is much greater when the soluble protein is present in the incubation. The inhibition is competitive with respect to methyl sterol substrate; cholesterol succinate, a water-soluble ester, is strongly inhibitory, K'i (ester)/K'm(substrate) approximately 0.2. Since end product inhibition of methyl sterol oxidase may be produced by accumulation of cholesterol or cholesterol metabolites, the soluble protein may participate in regulation of the activity of some microsomal enzymes of cholesterol biosynthesis.", "contents": "Purification of a soluble rat liver protein that stimulates microsomal 4-methyl sterol oxidase activity. A soluble rat liver protein that stimulates microsomal methyl sterol oxidase activity has been isolated and purified to homogeneity by salt fractionation, differential heat inactivation, two calcium phosphate gel association, and Sephadex filtration. The protein, as isolated, may be dissociated with detergent into subunits with a molecular weight of approximately 10,300, as determined electrophoretically. In addition to this stimulatory protein, postmicrosomal supernatant fraction of rat liver contains a low molecular weight methyl sterol oxidase inhibitor, possibly cholesterol ester, which is removed during protein purification. Purified soluble protein enhances the observed rate of oxidative attack of the methyl sterol substrates, 4, 4-dimethyl-5alpha-cholest-7-en-3beta-ol and 4alpha-methyl-5alpha-cholest-7-en-3beta-ol. Addition of increasing amounts of either partially purified or homogeneous soluble protein yields hyperbolic stimulation, from which a K'm of about 7 muM has been calculated (based on a monomeric molecular weight of 10,300). Sequential additions of the soluble protein yield equal increments of stimulation. These results are consistent with the suggestion that the soluble protein may be a reactant in the oxidative process. Methyl sterol oxidase is inhitited in vitro by cholesterol, several oxygenated sterols, and cholesterol esters. The extent of inhibition is much greater when the soluble protein is present in the incubation. The inhibition is competitive with respect to methyl sterol substrate; cholesterol succinate, a water-soluble ester, is strongly inhibitory, K'i (ester)/K'm(substrate) approximately 0.2. Since end product inhibition of methyl sterol oxidase may be produced by accumulation of cholesterol or cholesterol metabolites, the soluble protein may participate in regulation of the activity of some microsomal enzymes of cholesterol biosynthesis.", "PMID": 977591}
{"id": "pubmed23n0032_11189", "title": "Enzymatic reactions of methionine sulfoximine. Conversion to the corresponding alpha-imino and alpha-keto acids and to alpha-ketobutyrate and methane sulfinimide.", "content": "L-Methionine sulfoximine is a substrate of L-amino acid oxidase (Crotalus adamanteus), glutamine transaminase, and gamma-cystathionase. In the reaction catalyzed by L-amino acid oxidase, methionine sulfoximine is converted to aplph-imino-gamma-methylsulfoximinylbutyrate, which undergoes rapid gamma elimination yielding methane sulfinimide and 2-imino-3-butenoic acid. Methane sulfinimide is converted to methane sulfonamide, methane sulfinic acid, and methane sulfonic acid; 2-imino-3-butenoic acid is hydrolyzed to vinylglyoxylate, which polymerizes to an insoluble product. When the reaction is carried out in the presence of semicarbazide, the imine formed initially is quantitatively trapped as alpha-keto-gamma-methylsulfoximinylbutyrate semicarbazone, from which the free alpha-keto acid may be obtained. When the reaction is carried out in the presence of a mercaptan (RSH), a gamma exchange reaction occurs leading to formation of a new alpha-keto acid substituted in the gamma position by an SR-group; thus, alpha-keto-gamma-(beta-hydroxyethiol)butyric acid (S-(hydroxyethyl)-2-keto-4-mercaptobutyric acid) was obtained when L-methionine sulfoximine was oxidized in the presence of 2-mercaptoethanol, and enzymatic transamination of this alpha-keto acid with L-glutamine gave the new amino acid, L-omega-hydroxyethionine. The reaction of L-methionine sulfoximine with gamma-cystathionase yields 1 mol each of alpha-ketobutyrate and methane sulfinimide; the latter is hydrolyzed almost exclusively to methane sulfinic acid. Transamination of L-methionine sulfoximine yields the corresponding alpha-keto acid (alpha-keto-gamma-methylsulfoximinylbutyrate), which is stable. Some of these reactions may occur in vivo, and thus contribute to the toxicity of L-methionine sulfoximine.", "contents": "Enzymatic reactions of methionine sulfoximine. Conversion to the corresponding alpha-imino and alpha-keto acids and to alpha-ketobutyrate and methane sulfinimide. L-Methionine sulfoximine is a substrate of L-amino acid oxidase (Crotalus adamanteus), glutamine transaminase, and gamma-cystathionase. In the reaction catalyzed by L-amino acid oxidase, methionine sulfoximine is converted to aplph-imino-gamma-methylsulfoximinylbutyrate, which undergoes rapid gamma elimination yielding methane sulfinimide and 2-imino-3-butenoic acid. Methane sulfinimide is converted to methane sulfonamide, methane sulfinic acid, and methane sulfonic acid; 2-imino-3-butenoic acid is hydrolyzed to vinylglyoxylate, which polymerizes to an insoluble product. When the reaction is carried out in the presence of semicarbazide, the imine formed initially is quantitatively trapped as alpha-keto-gamma-methylsulfoximinylbutyrate semicarbazone, from which the free alpha-keto acid may be obtained. When the reaction is carried out in the presence of a mercaptan (RSH), a gamma exchange reaction occurs leading to formation of a new alpha-keto acid substituted in the gamma position by an SR-group; thus, alpha-keto-gamma-(beta-hydroxyethiol)butyric acid (S-(hydroxyethyl)-2-keto-4-mercaptobutyric acid) was obtained when L-methionine sulfoximine was oxidized in the presence of 2-mercaptoethanol, and enzymatic transamination of this alpha-keto acid with L-glutamine gave the new amino acid, L-omega-hydroxyethionine. The reaction of L-methionine sulfoximine with gamma-cystathionase yields 1 mol each of alpha-ketobutyrate and methane sulfinimide; the latter is hydrolyzed almost exclusively to methane sulfinic acid. Transamination of L-methionine sulfoximine yields the corresponding alpha-keto acid (alpha-keto-gamma-methylsulfoximinylbutyrate), which is stable. Some of these reactions may occur in vivo, and thus contribute to the toxicity of L-methionine sulfoximine.", "PMID": 977592}
{"id": "pubmed23n0032_11190", "title": "Characterization of carnitine acylcarnitine translocase system of heart mitochondria.", "content": "Mersalyl inhibited the respiration of heart mitochondria under conditions that required the transport of (-)-carnitine and acyl(-)-carnitines. The exchange of external carnitine and acylcarnitines for intramitochondrial carnitine was also inhibited by mersalyl and 1 mM mersalyl proved suitable for the inhibitor-stop assay of carnitine acylcarnitine translocase. The carnitine-carnitine and (-)-carnitine-acetyl(-)-carnitine exchanges involved a mole to mole exchange. The carnitine-carnitine exchange did not require energy. The carnitine acylcarnitine translocase resembles the Pi transport system in inhibition by mersalyl and N-ethylmaleimide and in lack of a cation requirement for activity; yet the two are not identical inasmuch as operation of only the former transport system was inhibited by long chain acyl(+)-carnitines. Additional results render it improbable that the transport of carnitine and acylcarnitines is catalyzed by any other known mitochondrial transport systems. The carnitine acylcarnitine translocase activity is unlikely to be shared by one of the carnitine acyltransferases because the mersalyl inhibition of carnitine palmitoyltransferase and carnitine acetyltransferase was noncompetitivcase. Rapid acetylation of intramitocondrial free (-)-carnitine occurred when acetyl-CoA was generated intramitochondrially but not with exogenous acetyl-CoA. Theese observations substantiate the view (Pande, S. V. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 883-887) that a system exists in mitochondria for the transport of carnitine and its esters and that the matrix has a pool of carnitine compounds which has access to that carnitine acyltransferase which is localized on the inner side of the inner mitochondrial membrane.", "contents": "Characterization of carnitine acylcarnitine translocase system of heart mitochondria. Mersalyl inhibited the respiration of heart mitochondria under conditions that required the transport of (-)-carnitine and acyl(-)-carnitines. The exchange of external carnitine and acylcarnitines for intramitochondrial carnitine was also inhibited by mersalyl and 1 mM mersalyl proved suitable for the inhibitor-stop assay of carnitine acylcarnitine translocase. The carnitine-carnitine and (-)-carnitine-acetyl(-)-carnitine exchanges involved a mole to mole exchange. The carnitine-carnitine exchange did not require energy. The carnitine acylcarnitine translocase resembles the Pi transport system in inhibition by mersalyl and N-ethylmaleimide and in lack of a cation requirement for activity; yet the two are not identical inasmuch as operation of only the former transport system was inhibited by long chain acyl(+)-carnitines. Additional results render it improbable that the transport of carnitine and acylcarnitines is catalyzed by any other known mitochondrial transport systems. The carnitine acylcarnitine translocase activity is unlikely to be shared by one of the carnitine acyltransferases because the mersalyl inhibition of carnitine palmitoyltransferase and carnitine acetyltransferase was noncompetitivcase. Rapid acetylation of intramitocondrial free (-)-carnitine occurred when acetyl-CoA was generated intramitochondrially but not with exogenous acetyl-CoA. Theese observations substantiate the view (Pande, S. V. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 883-887) that a system exists in mitochondria for the transport of carnitine and its esters and that the matrix has a pool of carnitine compounds which has access to that carnitine acyltransferase which is localized on the inner side of the inner mitochondrial membrane.", "PMID": 977593}
{"id": "pubmed23n0032_11191", "title": "Use of beta-parinaric acid, a novel fouorimetric probe, to determine characteristic temperatures of membranes and membrane lipids from cultured animal cells.", "content": "A naturally occurring fluorescent compound, beta-parinaric acid, was employed as a probe to measure the effects of temperature changes on plasma membrenes, microsomes, and mitochondria and on their respective lipids after isolation form LM cells grown in suspension culture. A computer-centered spectrofluorimenter simultaneously measured the absorbance, absorbance-corrected fluorescence, and relative fluorescence efficiency of beta-parinaric acid incorporated into the membranes or isolated membrane lipids. These parameters were measured as a function of temperature. The probe revealed five characteristic breaks or changes in slope with both the plasma membranes as well as their extracted lipids. These discontinuities occurred at approximately 18, 23, 31, 38, and 43 degrees. The other isolated subcellular organelles, microsomes, and mitochondria, as well as their respective isolated lipids, exhibited approximately the same characteristic temperatures (+/- 1 degree) as plasma membranes. Thus, these data negate one criterion of the theory that an asymmetric distribution of characteristic temperatures exist across the membranes of LM cells.", "contents": "Use of beta-parinaric acid, a novel fouorimetric probe, to determine characteristic temperatures of membranes and membrane lipids from cultured animal cells. A naturally occurring fluorescent compound, beta-parinaric acid, was employed as a probe to measure the effects of temperature changes on plasma membrenes, microsomes, and mitochondria and on their respective lipids after isolation form LM cells grown in suspension culture. A computer-centered spectrofluorimenter simultaneously measured the absorbance, absorbance-corrected fluorescence, and relative fluorescence efficiency of beta-parinaric acid incorporated into the membranes or isolated membrane lipids. These parameters were measured as a function of temperature. The probe revealed five characteristic breaks or changes in slope with both the plasma membranes as well as their extracted lipids. These discontinuities occurred at approximately 18, 23, 31, 38, and 43 degrees. The other isolated subcellular organelles, microsomes, and mitochondria, as well as their respective isolated lipids, exhibited approximately the same characteristic temperatures (+/- 1 degree) as plasma membranes. Thus, these data negate one criterion of the theory that an asymmetric distribution of characteristic temperatures exist across the membranes of LM cells.", "PMID": 977594}
{"id": "pubmed23n0032_11192", "title": "Physical properties of membranes isolated from tissue culture cells with altered phospholipid composition.", "content": "A choline-requiring strain of mouse fibroblast cells (LM cells) was cultured in suspension with choline, N,N'-dimethylethanolamine, N-monomethylethanolamine, or ethanolamine. These choline analogues were incorporated into membrane phospholipids as phosphatidyl-N,N'-dimethylethanolamine, phosphatidyl-N-monomethylethanolamine, and phosphatidylethanolamine. Plasma membranes, microsomes, mitochondria, and their respective lipids were isolated and the characteristic temperatures were determined by using two types of fluorescent probes: (a) beta-parinaric acid, a naturally occurring molecule, and (b) 8-anilino-1-naphthalene sulfonic acid, a synthetic organic fluorophore. A computer-centered spectrofluorimeter capable of simultaneous measurement of absorbance, absorbance-corrected fluorescence, and relative fluorescence efficiency was utilized for on-line measurement of all fluorescence parameters. Plots of absorbance corrected fluorescence or of relative fluorescence efficiency versus temperature revealed the same five characteristic temperatures with both types of probe. These characteristic temperatures were independent of the phospholipid composition of the LM suspension cell membranes or their extracted lipids. Plasma membranes, microsomes, and mitochondria containing analogue phospholipids had similar (+/- 1 degree) characteristic temperatures. The presence of analogue phopholipids altered the binding characteristics of beta-parinaric acid with plasma membranes and plasma membrane lipids of LM suspension cells. The equilibrium dissociation constant of plasma membranes and plasma membrane lipids was decreased 2- and 5-fold, respectively, when the cells had been supplemented with ethanolamine. The minimum number of phospholipid molecules per probe binding site was approximately constant in the intact plasma membrane but increased (2-fold) in the isolated plasma membrane lipids. The presence of analogue phospholipids also altered the interaction of 8-anilino-1-naphthalene sulfonic acid with LM cell membranes. The equilibrium dissociation constant of this probe interacting with mitochondrial lipid was decreased 40% by ethanolamine supplementation. The fluorescent properties of both probes were sensitive to the degree of methylation of the polar head group. The absolute values of absorbance-corrected fluorescence and relative fluorescence efficiency were different for each type of membrane from LM cells even with the same analogue supplement. Thus, it appears that LM cells maintain the characteristic temperatures which are a measure of the physical properties of their membranes, despite large alterations of the phospholipid polar head group composition.", "contents": "Physical properties of membranes isolated from tissue culture cells with altered phospholipid composition. A choline-requiring strain of mouse fibroblast cells (LM cells) was cultured in suspension with choline, N,N'-dimethylethanolamine, N-monomethylethanolamine, or ethanolamine. These choline analogues were incorporated into membrane phospholipids as phosphatidyl-N,N'-dimethylethanolamine, phosphatidyl-N-monomethylethanolamine, and phosphatidylethanolamine. Plasma membranes, microsomes, mitochondria, and their respective lipids were isolated and the characteristic temperatures were determined by using two types of fluorescent probes: (a) beta-parinaric acid, a naturally occurring molecule, and (b) 8-anilino-1-naphthalene sulfonic acid, a synthetic organic fluorophore. A computer-centered spectrofluorimeter capable of simultaneous measurement of absorbance, absorbance-corrected fluorescence, and relative fluorescence efficiency was utilized for on-line measurement of all fluorescence parameters. Plots of absorbance corrected fluorescence or of relative fluorescence efficiency versus temperature revealed the same five characteristic temperatures with both types of probe. These characteristic temperatures were independent of the phospholipid composition of the LM suspension cell membranes or their extracted lipids. Plasma membranes, microsomes, and mitochondria containing analogue phospholipids had similar (+/- 1 degree) characteristic temperatures. The presence of analogue phopholipids altered the binding characteristics of beta-parinaric acid with plasma membranes and plasma membrane lipids of LM suspension cells. The equilibrium dissociation constant of plasma membranes and plasma membrane lipids was decreased 2- and 5-fold, respectively, when the cells had been supplemented with ethanolamine. The minimum number of phospholipid molecules per probe binding site was approximately constant in the intact plasma membrane but increased (2-fold) in the isolated plasma membrane lipids. The presence of analogue phospholipids also altered the interaction of 8-anilino-1-naphthalene sulfonic acid with LM cell membranes. The equilibrium dissociation constant of this probe interacting with mitochondrial lipid was decreased 40% by ethanolamine supplementation. The fluorescent properties of both probes were sensitive to the degree of methylation of the polar head group. The absolute values of absorbance-corrected fluorescence and relative fluorescence efficiency were different for each type of membrane from LM cells even with the same analogue supplement. Thus, it appears that LM cells maintain the characteristic temperatures which are a measure of the physical properties of their membranes, despite large alterations of the phospholipid polar head group composition.", "PMID": 977595}
{"id": "pubmed23n0032_11193", "title": "Proteolytic cleavage of horse liver cytochrome b5. Primary structure of the heme-containing moiety.", "content": "The amino acid sequence of the NH2-terminal segment of horse cytochrome b5, containing the heme binding site, has been determined. A fragment, representing residues 7 through 90, was obtained by tryptic cleavage of native cytochrome b5. Chymotryptic cleavage of native cytochrome b5 yields a peptide containing residues 1 through 98. Contrary to native cytochrome b5, neither derivative showed binding to horse liver microsomal vesicles. The complete primary structure of the polar moiety has been deducted from automated and manual sequence analysis of peptides obtained from tryptic and chymotryptic digests of native cytochrome and apocytochrome preparations. Glutamyl residues at positions 41, 42, 47, and 48 appear to be replaced by aspartyl residues in some molecules. Such microheterogeneity is not observed at glutamyl residues at other positions. The native cytochrome b5 preparation contains a blocked NH2-terminal residue.", "contents": "Proteolytic cleavage of horse liver cytochrome b5. Primary structure of the heme-containing moiety. The amino acid sequence of the NH2-terminal segment of horse cytochrome b5, containing the heme binding site, has been determined. A fragment, representing residues 7 through 90, was obtained by tryptic cleavage of native cytochrome b5. Chymotryptic cleavage of native cytochrome b5 yields a peptide containing residues 1 through 98. Contrary to native cytochrome b5, neither derivative showed binding to horse liver microsomal vesicles. The complete primary structure of the polar moiety has been deducted from automated and manual sequence analysis of peptides obtained from tryptic and chymotryptic digests of native cytochrome and apocytochrome preparations. Glutamyl residues at positions 41, 42, 47, and 48 appear to be replaced by aspartyl residues in some molecules. Such microheterogeneity is not observed at glutamyl residues at other positions. The native cytochrome b5 preparation contains a blocked NH2-terminal residue.", "PMID": 977596}
{"id": "pubmed23n0032_11194", "title": "Spectral changes in bovine factor X associated with activation by the venom coagulant protein of Vipera russelli.", "content": "Bovine Factor X is a zymogen involved in blood coagulation that is converted to activated Factor X in the presence of Ca(II) by the coagulant protein of Russell's viper venom. To monitor structural transitions in Factor X during conversion to activated Factor X, the ultraviolet absorption, fluorescence emission, and circular dichroism spectra of activated Factor X and Factor X were compared. The ultraviolet absorption difference spectrum in the aromatic region comparing activated Factor X and Factor X indicates minima at 292.5, 285, and 278 nm and a small maximum at 305 nm; these differences are due to tryptophan and tyrosine perturbations. The activation of Factor X at 25 degrees in the presence of 8.3 mM CaCl2 with the use of Factor X:venom coagulant protein in molar rations of 1500:1 yielded a time-dependent increase in this spectrum which was linear for about 60 min and which temporally paralleled the development of activated Factor X activity. The binding of Ca(II) to factor X or activated Factor X is associated with a red-shifted tryptophan difference spectrum; however, this perturbation appears to make only a small contribution to the total perturbation observed during Factor X activation. Solvent perturbation studies in 20% glycerol suggest that an average of 3.1 tryptophan residues and 9.0 tyrosine residues are exposed to solvent in Factor X in 8.3 mM CaCl2 at pH 7.4; an additional 0.5 tryptophan residue and tyrosine reside become exposed to solvent during activation of Factor X in 8.3 mM CaCl2. The activation of Factor X by the venom coagulant protein is associated with a small red shift in the intrinsic tryptophan fluorescence emission spectrum. Far- and near-ultraviolet circular dichroism spectroscopy detected no difference between Factor X and activated Factor X. In summary, the activation of Factor X to activated Factor X appears associated with exposure of tryptophan and tyrosine side chains previously buried within the protein and with minimal changes in the secondary structur. These results suggest that conversion of Factor X to activated Factor X involves functionally important, but structurally subtle, changes in the three-dimentional structure.", "contents": "Spectral changes in bovine factor X associated with activation by the venom coagulant protein of Vipera russelli. Bovine Factor X is a zymogen involved in blood coagulation that is converted to activated Factor X in the presence of Ca(II) by the coagulant protein of Russell's viper venom. To monitor structural transitions in Factor X during conversion to activated Factor X, the ultraviolet absorption, fluorescence emission, and circular dichroism spectra of activated Factor X and Factor X were compared. The ultraviolet absorption difference spectrum in the aromatic region comparing activated Factor X and Factor X indicates minima at 292.5, 285, and 278 nm and a small maximum at 305 nm; these differences are due to tryptophan and tyrosine perturbations. The activation of Factor X at 25 degrees in the presence of 8.3 mM CaCl2 with the use of Factor X:venom coagulant protein in molar rations of 1500:1 yielded a time-dependent increase in this spectrum which was linear for about 60 min and which temporally paralleled the development of activated Factor X activity. The binding of Ca(II) to factor X or activated Factor X is associated with a red-shifted tryptophan difference spectrum; however, this perturbation appears to make only a small contribution to the total perturbation observed during Factor X activation. Solvent perturbation studies in 20% glycerol suggest that an average of 3.1 tryptophan residues and 9.0 tyrosine residues are exposed to solvent in Factor X in 8.3 mM CaCl2 at pH 7.4; an additional 0.5 tryptophan residue and tyrosine reside become exposed to solvent during activation of Factor X in 8.3 mM CaCl2. The activation of Factor X by the venom coagulant protein is associated with a small red shift in the intrinsic tryptophan fluorescence emission spectrum. Far- and near-ultraviolet circular dichroism spectroscopy detected no difference between Factor X and activated Factor X. In summary, the activation of Factor X to activated Factor X appears associated with exposure of tryptophan and tyrosine side chains previously buried within the protein and with minimal changes in the secondary structur. These results suggest that conversion of Factor X to activated Factor X involves functionally important, but structurally subtle, changes in the three-dimentional structure.", "PMID": 977597}
{"id": "pubmed23n0032_11195", "title": "Nuclear relaxation and gelation study of the interaction of organophosphates with human normal and sickle hemoglobins. In vitro gelation of sickle oxyhemoglobin in the presence of inositol hexaphosphate.", "content": "31P relaxation studies reveal a 3-fold enhancement of the longitudinal relaxation rate of both phosphoryl groups of hemoglobin-bound 2,3-bisphosphoglycerate upon conversion of methemoglobin to fluoromethemoglobin presumably due to an order of magnitude increase in the electron spin relaxation time. The enhancement of the longitudinal components of 31P relaxation (T(-1)1pr) upon binding to hemoglobin is not exchange-limited, since it is more than an order of magnitude smaller than the effect observed on the transverse components (T(-1)2pr). From the observed paramagnetic component, T(-1)1M, of the bound state relaxation rate of 2,3-bisphosphoglycerate, using the correlation time tau s obtained from the frequency dependence of water proton relaxation, we obtained an NMR root mean sixth distance from the four heme iron atoms to each of the 31P nuclei of 24 +/- 1 A. This is in excellent agreement with the x-ray crystallographic determination of this distance of (25 +/- 1) A in the 2,3-bisphosphoglycerate-deoxyhemoglobin complex, indicating that the spatial disposition of the allosteric site in the deoxy and oxy conformations of hemoglobin relative to the various heme irons may be the same, and that the same protein groups may be involved in binding 2,3-bisphosphoglycerate to the two forms of hemoglobin. Water proton relaxation studies reveal the existence of different conformational states of methemoglobins with 2,3-bisphosphoglycerate and inositol hexaphosphate. Inositol hexaphosphate alters the conformation to a strained (T) state with deoxy-like quaternary and tertiary globin structure as indicated by the finding that equimolar amounts of inositol heasphosphate induce gelation in a 4 mM sickle methemoglobin solution at temperatures greater than or equal 24 degrees. More interestingly, oxyhemoglobin S shows an identical thermodynamically reversible gelation behavior, with the same transition temperature (24 degrees), arguing against a mutual coupling of the protein conformation and the heme spin state in functional ferrohemoglobins. High ionic strengths (approximately 1 M) and pH values above neutrality block inositol hexaphosphate induced gelation of sickle met- and oxyhemoglobins. Unlike inositol hexaphosphate, the presence of saturating amounts of 2,3-bisphosphoglycerate does not promote gelation of a 4 mM met- or oxyhemoglobin S solution.", "contents": "Nuclear relaxation and gelation study of the interaction of organophosphates with human normal and sickle hemoglobins. In vitro gelation of sickle oxyhemoglobin in the presence of inositol hexaphosphate. 31P relaxation studies reveal a 3-fold enhancement of the longitudinal relaxation rate of both phosphoryl groups of hemoglobin-bound 2,3-bisphosphoglycerate upon conversion of methemoglobin to fluoromethemoglobin presumably due to an order of magnitude increase in the electron spin relaxation time. The enhancement of the longitudinal components of 31P relaxation (T(-1)1pr) upon binding to hemoglobin is not exchange-limited, since it is more than an order of magnitude smaller than the effect observed on the transverse components (T(-1)2pr). From the observed paramagnetic component, T(-1)1M, of the bound state relaxation rate of 2,3-bisphosphoglycerate, using the correlation time tau s obtained from the frequency dependence of water proton relaxation, we obtained an NMR root mean sixth distance from the four heme iron atoms to each of the 31P nuclei of 24 +/- 1 A. This is in excellent agreement with the x-ray crystallographic determination of this distance of (25 +/- 1) A in the 2,3-bisphosphoglycerate-deoxyhemoglobin complex, indicating that the spatial disposition of the allosteric site in the deoxy and oxy conformations of hemoglobin relative to the various heme irons may be the same, and that the same protein groups may be involved in binding 2,3-bisphosphoglycerate to the two forms of hemoglobin. Water proton relaxation studies reveal the existence of different conformational states of methemoglobins with 2,3-bisphosphoglycerate and inositol hexaphosphate. Inositol hexaphosphate alters the conformation to a strained (T) state with deoxy-like quaternary and tertiary globin structure as indicated by the finding that equimolar amounts of inositol heasphosphate induce gelation in a 4 mM sickle methemoglobin solution at temperatures greater than or equal 24 degrees. More interestingly, oxyhemoglobin S shows an identical thermodynamically reversible gelation behavior, with the same transition temperature (24 degrees), arguing against a mutual coupling of the protein conformation and the heme spin state in functional ferrohemoglobins. High ionic strengths (approximately 1 M) and pH values above neutrality block inositol hexaphosphate induced gelation of sickle met- and oxyhemoglobins. Unlike inositol hexaphosphate, the presence of saturating amounts of 2,3-bisphosphoglycerate does not promote gelation of a 4 mM met- or oxyhemoglobin S solution.", "PMID": 977598}
{"id": "pubmed23n0032_11196", "title": "Some properties of purified skeletal muscle alpha-actinin.", "content": "Highly purified alpha-actinin can be made by using the low ionic strength extraction procedure previously described (Arakawa N., Robson, R. M., and Goll, D. E. (1970) Biochim. Biophys. Acta 200, 284-295) and then subjecting the crude alpha-actinin fraction obtained with this extraction procedure to successive chromatography on DEAE-cellulose and hydroxyapatite. Hydrozyapatite chromatography specifically removes a protein having a subunit molecular weight of 42,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Hydroxyapatite-purified alpha-actinin sediments entirely as a 6.21 S boundary in the analytical ultracentrifuge with no trace of the small 9 to 10 S boundary seen in earlier alpha-actinin preparations purified by DEAE-cellulose chromatography. In 100 mM KCl, 20 mM Tris-acetate, pH 7.5, hydroxyapatite-purified alpha-actinin has a diffusion coefficient (D020,w) of 2.71 X 10(-7) cm2-s-1, an intrinsic viscosity of 20.6 ml-g-1, a molecular weight of 201,000 +/- 4,300 (plus or minus least squares standard error) as determined by sedimentation equilibrium, and a molecular weight of 210,000 as determined by sedimentation diffusion. In 6 M guanidine HCl, hydroxyapatite-purified alpha-actinin has a molecular weight of 106,000 +/- 6,300 as determined by sedimentation equilibrium and a molecular weight of 100,000 as determined by a calibrated 4% agarose gel permeation column. SDS-polyacrylamide gel electrophoresis gives a molecular weight of 96,000 to 100,000 for hydroxyapatite-purified alpha-actinin. Rod-shaped particles 44 X 390 to 400 A are seen in electron micrographs of negatively stained alpha-actinin. By assuming 45% hydration and a molecular weight of 206,000, dimensions of approximately 40 X 500 A can be calculated for the alpha-actinin molecule by using either s 020, w, D 020, w, intrinsic viscosity, or a calibrated 6% agarose gel permeation column. Hydroxyapatite-purified alpha-actinin has an alpha-helical content of 74% as measured by circular dichroism at 208 nm.", "contents": "Some properties of purified skeletal muscle alpha-actinin. Highly purified alpha-actinin can be made by using the low ionic strength extraction procedure previously described (Arakawa N., Robson, R. M., and Goll, D. E. (1970) Biochim. Biophys. Acta 200, 284-295) and then subjecting the crude alpha-actinin fraction obtained with this extraction procedure to successive chromatography on DEAE-cellulose and hydroxyapatite. Hydrozyapatite chromatography specifically removes a protein having a subunit molecular weight of 42,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Hydroxyapatite-purified alpha-actinin sediments entirely as a 6.21 S boundary in the analytical ultracentrifuge with no trace of the small 9 to 10 S boundary seen in earlier alpha-actinin preparations purified by DEAE-cellulose chromatography. In 100 mM KCl, 20 mM Tris-acetate, pH 7.5, hydroxyapatite-purified alpha-actinin has a diffusion coefficient (D020,w) of 2.71 X 10(-7) cm2-s-1, an intrinsic viscosity of 20.6 ml-g-1, a molecular weight of 201,000 +/- 4,300 (plus or minus least squares standard error) as determined by sedimentation equilibrium, and a molecular weight of 210,000 as determined by sedimentation diffusion. In 6 M guanidine HCl, hydroxyapatite-purified alpha-actinin has a molecular weight of 106,000 +/- 6,300 as determined by sedimentation equilibrium and a molecular weight of 100,000 as determined by a calibrated 4% agarose gel permeation column. SDS-polyacrylamide gel electrophoresis gives a molecular weight of 96,000 to 100,000 for hydroxyapatite-purified alpha-actinin. Rod-shaped particles 44 X 390 to 400 A are seen in electron micrographs of negatively stained alpha-actinin. By assuming 45% hydration and a molecular weight of 206,000, dimensions of approximately 40 X 500 A can be calculated for the alpha-actinin molecule by using either s 020, w, D 020, w, intrinsic viscosity, or a calibrated 6% agarose gel permeation column. Hydroxyapatite-purified alpha-actinin has an alpha-helical content of 74% as measured by circular dichroism at 208 nm.", "PMID": 977599}
{"id": "pubmed23n0032_11197", "title": "Banded figers in high temperature coacervates of elastin peptides.", "content": "Electron micrographs of negatively stained coacervates of the synthetic polypentapeptide of propoelastin and of alpha-elastin exhibit banded fibers when the coacervates are formed, stained, and dried at temperatures greater than 50 degrees. This apparent increase in order occurs at the same temperature as an increase in order in aqueous solution and as a change in the volume expansion coefficient of fibrous elastin.", "contents": "Banded figers in high temperature coacervates of elastin peptides. Electron micrographs of negatively stained coacervates of the synthetic polypentapeptide of propoelastin and of alpha-elastin exhibit banded fibers when the coacervates are formed, stained, and dried at temperatures greater than 50 degrees. This apparent increase in order occurs at the same temperature as an increase in order in aqueous solution and as a change in the volume expansion coefficient of fibrous elastin.", "PMID": 977600}
{"id": "pubmed23n0032_11198", "title": "Preparation and characterization of wear debris of orthopedic materials for biocompatibility studies.", "content": "In order to test the biocompatibility of wear debris of orthopedic materials, a method has been developed to produce artificial debris of stainless steel, cobalt-chromium alloy, and high-density polyethylene. In this process, called the accelerated rubbing process, two cylindrical blocks of the same alloy were held under normal pressure in triply distilled water. One block remained stationary while the other rotated at approximately 4000 rpm. The water with the metal debris was recirculated between the rubbing surfaces. To prepare debris from polyethylene, a stainless steel block was rubbed over a polyethylene block submersed in liquid nitrogen. The shape of the metal debris was granular, whereas the polyethylene debris had a shred-like shape. The size distributions were determined from scanning electron micrographs, and it was found that the particles of the metal debris (stainless steel or cobalt-chrome alloy) ranged in sizes from 0.1-10 mu with 75% of all particles less than 2 mu in diameter. The size of polyethylene debris, expressed as the volume of an ellipsoid, ranged from 10-20,000 mu3 with 45% of all particles in the range of 400-2500 mu3. The x-ray diffraction pattern revealed that the debris of stainless steel consisted primarily of the face-centered cubic (fcc) alloy with small amounts of body-centered cubic (bcc) alloy and Fe3O4, whereas the cobalt-chrome debris consisted of the fcc alloy with a small amount of Cr2O3. The structure of the polyethylene debris was quite similar to that of the solid sample used in its preparation. The percent crystallinity Pc and the size of the crystalline regions D were slightly smaller in the debris than in the solid sample, i.e., Pc = 52 and 55%, D = 90 and 130 A, in the debris and the solid, respectively. To test the suitability of the artificial debris for biocompatibility studies, stainless steel debris was implanted into knees of adult female New Zealand rabbits. Bacterial challenge studies indicated prolongation, but not increased susceptibility to infection.", "contents": "Preparation and characterization of wear debris of orthopedic materials for biocompatibility studies. In order to test the biocompatibility of wear debris of orthopedic materials, a method has been developed to produce artificial debris of stainless steel, cobalt-chromium alloy, and high-density polyethylene. In this process, called the accelerated rubbing process, two cylindrical blocks of the same alloy were held under normal pressure in triply distilled water. One block remained stationary while the other rotated at approximately 4000 rpm. The water with the metal debris was recirculated between the rubbing surfaces. To prepare debris from polyethylene, a stainless steel block was rubbed over a polyethylene block submersed in liquid nitrogen. The shape of the metal debris was granular, whereas the polyethylene debris had a shred-like shape. The size distributions were determined from scanning electron micrographs, and it was found that the particles of the metal debris (stainless steel or cobalt-chrome alloy) ranged in sizes from 0.1-10 mu with 75% of all particles less than 2 mu in diameter. The size of polyethylene debris, expressed as the volume of an ellipsoid, ranged from 10-20,000 mu3 with 45% of all particles in the range of 400-2500 mu3. The x-ray diffraction pattern revealed that the debris of stainless steel consisted primarily of the face-centered cubic (fcc) alloy with small amounts of body-centered cubic (bcc) alloy and Fe3O4, whereas the cobalt-chrome debris consisted of the fcc alloy with a small amount of Cr2O3. The structure of the polyethylene debris was quite similar to that of the solid sample used in its preparation. The percent crystallinity Pc and the size of the crystalline regions D were slightly smaller in the debris than in the solid sample, i.e., Pc = 52 and 55%, D = 90 and 130 A, in the debris and the solid, respectively. To test the suitability of the artificial debris for biocompatibility studies, stainless steel debris was implanted into knees of adult female New Zealand rabbits. Bacterial challenge studies indicated prolongation, but not increased susceptibility to infection.", "PMID": 977601}
{"id": "pubmed23n0032_11199", "title": "Biocompatibility of nitinol alloy as an implant material.", "content": "The biocompatibility of nitinol alloy as a potential implant material was investigated through in vivo studies on beagles. A high-purity alloy was fabricated into prototype bone plates and implanted into the femurs of beagles. Commercial cobalt-chromium (Co-Cr) alloy bone plates served as reference controls, an additional control data were obtained from beagles subjected to \"sham\" operations. The bone plates were removed from the animals and examined after exposures of 3, 6, 12, and 17 months. There was no evidence of either localized or of general corrosion on the surfaces of the bone plates and screws. Gross clinical, radiological, and morphological observations of the tissue at the implantation sites during the autopsies uncovered no signs of adverse tissue reactions resulting from the implants. Histological analyses were performed on samples of muscle and bone adjacent to the implantation sites, and of tissues removed from such organs as the liver, spleen, brain, and kidneys. No significant differences were noted between samples taken from controls and those taken from dogs exposed to the implants. Neutron activation analyses were carried out on suitable samples. The analysis data suggest that there is no metallic contamination in the organs due to the implants; however, there does appear to be some chromium contamination from the Co-Cr alloy implants in the adjacent bone. On the basis of the totality of the data, it is concluded that nitinol alloy is sufficiently compatible with dog tissue to warrant further investigation of its potential as a biomaterial.", "contents": "Biocompatibility of nitinol alloy as an implant material. The biocompatibility of nitinol alloy as a potential implant material was investigated through in vivo studies on beagles. A high-purity alloy was fabricated into prototype bone plates and implanted into the femurs of beagles. Commercial cobalt-chromium (Co-Cr) alloy bone plates served as reference controls, an additional control data were obtained from beagles subjected to \"sham\" operations. The bone plates were removed from the animals and examined after exposures of 3, 6, 12, and 17 months. There was no evidence of either localized or of general corrosion on the surfaces of the bone plates and screws. Gross clinical, radiological, and morphological observations of the tissue at the implantation sites during the autopsies uncovered no signs of adverse tissue reactions resulting from the implants. Histological analyses were performed on samples of muscle and bone adjacent to the implantation sites, and of tissues removed from such organs as the liver, spleen, brain, and kidneys. No significant differences were noted between samples taken from controls and those taken from dogs exposed to the implants. Neutron activation analyses were carried out on suitable samples. The analysis data suggest that there is no metallic contamination in the organs due to the implants; however, there does appear to be some chromium contamination from the Co-Cr alloy implants in the adjacent bone. On the basis of the totality of the data, it is concluded that nitinol alloy is sufficiently compatible with dog tissue to warrant further investigation of its potential as a biomaterial.", "PMID": 977602}
{"id": "pubmed23n0032_11200", "title": "In vitro effects of bone cement on S. aureus growth and phagocytosis and glucose metabolism of macrophage: a preliminary study.", "content": "A preliminary study of the effects of Surgical Simplex, mixed for various time periods, was performed on S. aureus growth rate, and phagocytosis and glucose metabolism of macrophage in vitro. Quantitatively, no significant effects of the mixture on S. aureus growth rate were observed. The cement mixed for less than 4 min tended to cause adverse effects on phagocytosis rate and hexose monophosphate shunt activity of macrophage. This information may not be directly applicable to in vivo, but in vitro the cement mixture in a certain situation can cause decreased phagocytosis and hexose monophosphate shunt activity of macrophage.", "contents": "In vitro effects of bone cement on S. aureus growth and phagocytosis and glucose metabolism of macrophage: a preliminary study. A preliminary study of the effects of Surgical Simplex, mixed for various time periods, was performed on S. aureus growth rate, and phagocytosis and glucose metabolism of macrophage in vitro. Quantitatively, no significant effects of the mixture on S. aureus growth rate were observed. The cement mixed for less than 4 min tended to cause adverse effects on phagocytosis rate and hexose monophosphate shunt activity of macrophage. This information may not be directly applicable to in vivo, but in vitro the cement mixture in a certain situation can cause decreased phagocytosis and hexose monophosphate shunt activity of macrophage.", "PMID": 977603}
{"id": "pubmed23n0032_11201", "title": "A unified mathematical model for diffusion from drug-polymer composite tablets.", "content": "The derivation and experimental verification of a unified mathematical model for the estimation of drug release rate from drug-polymer composite tablets are presented. Cylindrical coordinates are utilized in the solution of the diffusion equation for a three-dimensional system. The model is applicable to tablets that range from the shape of a flat disk (radius greater than thickness) to that of a cylindrical rod (radius less than thickness). The general solution for the fraction of drug released at a time t is (see article). This approach to a three-dimensional system, utilizing cylindrical coordinates, presents a comprehensive method for the estimation of drug release rates from sustained release tablets with drug distributed homogeneously throughout a polymer matrix. The calculated and experimental drug diffusion rate of pyrimethamine from pyrimethamine-silicone rubber composite tablets that range in shape from that of a disk to a cylinder, and of hydrocortisone from EVA, polycaprolactone, and PVA terpolymer, are compared.", "contents": "A unified mathematical model for diffusion from drug-polymer composite tablets. The derivation and experimental verification of a unified mathematical model for the estimation of drug release rate from drug-polymer composite tablets are presented. Cylindrical coordinates are utilized in the solution of the diffusion equation for a three-dimensional system. The model is applicable to tablets that range from the shape of a flat disk (radius greater than thickness) to that of a cylindrical rod (radius less than thickness). The general solution for the fraction of drug released at a time t is (see article). This approach to a three-dimensional system, utilizing cylindrical coordinates, presents a comprehensive method for the estimation of drug release rates from sustained release tablets with drug distributed homogeneously throughout a polymer matrix. The calculated and experimental drug diffusion rate of pyrimethamine from pyrimethamine-silicone rubber composite tablets that range in shape from that of a disk to a cylinder, and of hydrocortisone from EVA, polycaprolactone, and PVA terpolymer, are compared.", "PMID": 977604}
{"id": "pubmed23n0032_11202", "title": "Polymer-ceramic composite for tooth-root implant.", "content": "A new polymer-ceramic composite suitable for tooth-root implants has been developed in this study. This material exhibited the desirable combination of good mechanical properties, controlled porosity, and ease of processing. A thermal processing technique was utilized to polymerize acrylic acid (AA) in the presence of either 0.3 or 0.05 mu alumina particles. Porosity and pore size distribution were influenced by the alumina particle size and the processing technique. For a 50 vol % AA solution, the composite had an average compressive strength of 18,000 psi and 38% porosity when 0.3 mu filler particles were used. In comparison, the 0.05 mu alumina-filled composite had an average compressive strength of 28,000 psi with a 15% porosity. Data on the physical and structural characteristics of the composite are presented in this study. Based on these results, the composite material shows good potential for use in tooth-root implants as well as other orthopedic implant applications.", "contents": "Polymer-ceramic composite for tooth-root implant. A new polymer-ceramic composite suitable for tooth-root implants has been developed in this study. This material exhibited the desirable combination of good mechanical properties, controlled porosity, and ease of processing. A thermal processing technique was utilized to polymerize acrylic acid (AA) in the presence of either 0.3 or 0.05 mu alumina particles. Porosity and pore size distribution were influenced by the alumina particle size and the processing technique. For a 50 vol % AA solution, the composite had an average compressive strength of 18,000 psi and 38% porosity when 0.3 mu filler particles were used. In comparison, the 0.05 mu alumina-filled composite had an average compressive strength of 28,000 psi with a 15% porosity. Data on the physical and structural characteristics of the composite are presented in this study. Based on these results, the composite material shows good potential for use in tooth-root implants as well as other orthopedic implant applications.", "PMID": 977605}
{"id": "pubmed23n0032_11203", "title": "In vitro corrosion of dental amalgam phases.", "content": "The effects of corrosion on the major phases of dental amalgam microstructure have been studied in vitro on samples prepared by electroplating mercury on Ag3Sn. Tests were made in synthetic saliva, and samples were examined in a scanning electron microscope before and after the exposure. Simple immersion had little effect on the phases. Contact of amalgam with gold increased the probability of deterioation of the gamma2 phase. By anodic polarization beyond the breakdown potential, the gamma2 phase was selectively dissolved. Under conditions simulating a crevice, the gamma2 phase was dissolved and the gamma1 phase suffered morphological changes.", "contents": "In vitro corrosion of dental amalgam phases. The effects of corrosion on the major phases of dental amalgam microstructure have been studied in vitro on samples prepared by electroplating mercury on Ag3Sn. Tests were made in synthetic saliva, and samples were examined in a scanning electron microscope before and after the exposure. Simple immersion had little effect on the phases. Contact of amalgam with gold increased the probability of deterioation of the gamma2 phase. By anodic polarization beyond the breakdown potential, the gamma2 phase was selectively dissolved. Under conditions simulating a crevice, the gamma2 phase was dissolved and the gamma1 phase suffered morphological changes.", "PMID": 977606}
{"id": "pubmed23n0032_11204", "title": "Fracture characteristics of acrylic bone cements. I. Fracture toughness.", "content": "The vital first phase of the overall materials study to protract the life of the total joint replacements is the identification of the fracture toughness and fatigue properties of bone cements. Information gained from fatigue testing, performed in a manner which simmulates in vivo conditions, and fracture toughness, which is a measure of the propensity of a crack to propagate, is the first step towards the prediction of the life of the total joint replacement. This study is concerned with the fracture toughness of Zimmer and Simplex-P cold-curing bone cements. Following cement fabrication conditions which closely approximate clinical procedures, fracture toughness testing was conducted on cement specimens which were immersed in bovine serum at 37 degrees C in order to simulate in vivo conditions. In addition, a similar study was completed on specimens, tested in air at ambient temperature for purposes of comparison. Results of this procedure, when analyzed by a Student's t-test at the 95% confidence level with eight degrees of freedom, indicate that both Zimmer and Simplex-P exhibit a higher fracture toughness in the simulated physiological environment. In order to determine whether the addition of barium sulfate to these cements compromises the fracture toughness, the above described testing rationale was repeated, indicating the existence of a complicated relationship between the different testing environments and barium sulfate. The importance of these results lies in the fact that an increased fracture toughness indicates that a cement will inherently exhibit a greater degree of resistance to the propagation of cracks, which could contribute to the ultimate failure of the total joint replacement.", "contents": "Fracture characteristics of acrylic bone cements. I. Fracture toughness. The vital first phase of the overall materials study to protract the life of the total joint replacements is the identification of the fracture toughness and fatigue properties of bone cements. Information gained from fatigue testing, performed in a manner which simmulates in vivo conditions, and fracture toughness, which is a measure of the propensity of a crack to propagate, is the first step towards the prediction of the life of the total joint replacement. This study is concerned with the fracture toughness of Zimmer and Simplex-P cold-curing bone cements. Following cement fabrication conditions which closely approximate clinical procedures, fracture toughness testing was conducted on cement specimens which were immersed in bovine serum at 37 degrees C in order to simulate in vivo conditions. In addition, a similar study was completed on specimens, tested in air at ambient temperature for purposes of comparison. Results of this procedure, when analyzed by a Student's t-test at the 95% confidence level with eight degrees of freedom, indicate that both Zimmer and Simplex-P exhibit a higher fracture toughness in the simulated physiological environment. In order to determine whether the addition of barium sulfate to these cements compromises the fracture toughness, the above described testing rationale was repeated, indicating the existence of a complicated relationship between the different testing environments and barium sulfate. The importance of these results lies in the fact that an increased fracture toughness indicates that a cement will inherently exhibit a greater degree of resistance to the propagation of cracks, which could contribute to the ultimate failure of the total joint replacement.", "PMID": 977607}
{"id": "pubmed23n0032_11205", "title": "Congenital dislocation of the hip. Use of the Pavlik harness in the child during the first six months of life.", "content": "From 1968 to 1972, twenty-three infants under six months old with twenty-seven dislocated hips were treated with a Pavlik harness. All the dislocations except three were successfully reduced. Only one child required hospitalization. All the patients were followed for more than two years. All but three of the hips were clinically and roentgenographically normal at follow-up, and none had avascular necrosis. In infants, the Pavlik harness successfully utilizes the principle of reduction in flexion, avoiding forced abduction.", "contents": "Congenital dislocation of the hip. Use of the Pavlik harness in the child during the first six months of life. From 1968 to 1972, twenty-three infants under six months old with twenty-seven dislocated hips were treated with a Pavlik harness. All the dislocations except three were successfully reduced. Only one child required hospitalization. All the patients were followed for more than two years. All but three of the hips were clinically and roentgenographically normal at follow-up, and none had avascular necrosis. In infants, the Pavlik harness successfully utilizes the principle of reduction in flexion, avoiding forced abduction.", "PMID": 977608}
{"id": "pubmed23n0032_11206", "title": "Results of phalangectomy of the fifth toe for hammertoe. The Ruiz-Mora procedure.", "content": "The Ruiz-Mora procedure has been advocated for the correction of hammertoe deformities and congenital overlapping of the fifth toe, primarily on empirical grounds. This is a review of forty-one such procedures in which the operation was used for correction of a hammertoe deformity of the fifth toe with a painful corn overlying the proximal interphalangeal joint. Twenty-two patients with thirty-one procedures were followed for an average of 3.5 years. All of the patients had complete relief of symptoms and correction of deformity. However, in 23 per cent of the patients a painful prominent head of the fifth metartarsal or a bunionette developed, and in 32 per cent a hammertoe deformity of the adjacent fourth toe developed with a painful corn either over the proximal interphalangeal joint or on the tip of the fourth toe. These problems seem to be related to excessive shortening of the the fifth toe. Less bone resection (resection of only the head and neck of the proximal phalanx) might be preferable and might not lead to these complications.", "contents": "Results of phalangectomy of the fifth toe for hammertoe. The Ruiz-Mora procedure. The Ruiz-Mora procedure has been advocated for the correction of hammertoe deformities and congenital overlapping of the fifth toe, primarily on empirical grounds. This is a review of forty-one such procedures in which the operation was used for correction of a hammertoe deformity of the fifth toe with a painful corn overlying the proximal interphalangeal joint. Twenty-two patients with thirty-one procedures were followed for an average of 3.5 years. All of the patients had complete relief of symptoms and correction of deformity. However, in 23 per cent of the patients a painful prominent head of the fifth metartarsal or a bunionette developed, and in 32 per cent a hammertoe deformity of the adjacent fourth toe developed with a painful corn either over the proximal interphalangeal joint or on the tip of the fourth toe. These problems seem to be related to excessive shortening of the the fifth toe. Less bone resection (resection of only the head and neck of the proximal phalanx) might be preferable and might not lead to these complications.", "PMID": 977609}
{"id": "pubmed23n0032_11207", "title": "The wick catheter technique for measurement of intramuscular pressure. A new research and clinical tool.", "content": "The wick catheter technique was developed in 1968 for measurement of subcutaneous pressure and has been modified for easy intramuscular insertion and continuous recording of interstitial fluid pressure in animals and humans. Studies in dogs of the anterolateral compartment of the leg in simulation of the compartment syndrome showed the technique to be accurate and reproducible. The wick catheter technique is capable of important clinical applications in the diagnosis and treatment of acute and chronic compartment syndromes.", "contents": "The wick catheter technique for measurement of intramuscular pressure. A new research and clinical tool. The wick catheter technique was developed in 1968 for measurement of subcutaneous pressure and has been modified for easy intramuscular insertion and continuous recording of interstitial fluid pressure in animals and humans. Studies in dogs of the anterolateral compartment of the leg in simulation of the compartment syndrome showed the technique to be accurate and reproducible. The wick catheter technique is capable of important clinical applications in the diagnosis and treatment of acute and chronic compartment syndromes.", "PMID": 977611}
{"id": "pubmed23n0032_11208", "title": "Prevention of fatal pulmonary thromboembolism by heparin prophylaxis after surgery for hip fractures.", "content": "From 1960 through 1975, 337 patients with surgically treated acute fracture of the hip received subcutaneously administered heparin to prevent thromboembolic disease according to various regimens. Four hundred and three patients received no heparin. The incidence of fatal pulmonary embolism was 3.5 per cent in the 403 patients who reveived no heparin and 0.0 per cent in the 147 patients who were treated by the currently used regimen of prophylaxis, as follows: With the dose modified according to the coagulometer-test time, patients received 2,500 units on admission and every six hours until the day before operation. Then they were given 5,000 to 10,000 units eight to ten hours before surgery and 2,500 units every six hours after surgery until they were fully mobilized.", "contents": "Prevention of fatal pulmonary thromboembolism by heparin prophylaxis after surgery for hip fractures. From 1960 through 1975, 337 patients with surgically treated acute fracture of the hip received subcutaneously administered heparin to prevent thromboembolic disease according to various regimens. Four hundred and three patients received no heparin. The incidence of fatal pulmonary embolism was 3.5 per cent in the 403 patients who reveived no heparin and 0.0 per cent in the 147 patients who were treated by the currently used regimen of prophylaxis, as follows: With the dose modified according to the coagulometer-test time, patients received 2,500 units on admission and every six hours until the day before operation. Then they were given 5,000 to 10,000 units eight to ten hours before surgery and 2,500 units every six hours after surgery until they were fully mobilized.", "PMID": 977620}
{"id": "pubmed23n0032_11209", "title": "Thromboembolism following total hip-replacement arthroplasty. The efficicy of dextran-aspirin and dextran-warfarin in prophylaxis.", "content": "The efficacy of two antithrombotic regimens, combined dextran and aspirin and combined dextran and warfarin, was analyzed by comparing the incidence of thromboembolism following total hip replacement in two groups of similar patients. Of the 427 who received dextran and aspirin, 7 per cent had thromboembolic complications, including one case of fatal pulmonary embolus and one case of recurrent emboli that required vena caval ligation, and 15 per cent had wound-healing complications. Of the 197 patients who received dextran and warfarin, 5 per cent had thromboembolism and 24 per cent had wound healing complications. Although both prophylactic regimens seemed effective, dextran and aspirin appeared less effective in reducing thromboembolic complications than dextran and warfarin, but there were fewer wound complications in that group. One-fourth of the patients on dextran-warfarin were not adequately anticoagulated despite close supervision. In forty-five patients with a history of thromboembolism who were excluded from the study and analyzed separately, warfarin alone and the two described regimens were equally ineffective in preventing thromboembolism, and the incidence of thromboembolic complications was high. Dextran-aspirin and dextran-warfarin appear to be satisfactory and relatively simple methods of thromboembolic prophylaxis.", "contents": "Thromboembolism following total hip-replacement arthroplasty. The efficicy of dextran-aspirin and dextran-warfarin in prophylaxis. The efficacy of two antithrombotic regimens, combined dextran and aspirin and combined dextran and warfarin, was analyzed by comparing the incidence of thromboembolism following total hip replacement in two groups of similar patients. Of the 427 who received dextran and aspirin, 7 per cent had thromboembolic complications, including one case of fatal pulmonary embolus and one case of recurrent emboli that required vena caval ligation, and 15 per cent had wound-healing complications. Of the 197 patients who received dextran and warfarin, 5 per cent had thromboembolism and 24 per cent had wound healing complications. Although both prophylactic regimens seemed effective, dextran and aspirin appeared less effective in reducing thromboembolic complications than dextran and warfarin, but there were fewer wound complications in that group. One-fourth of the patients on dextran-warfarin were not adequately anticoagulated despite close supervision. In forty-five patients with a history of thromboembolism who were excluded from the study and analyzed separately, warfarin alone and the two described regimens were equally ineffective in preventing thromboembolism, and the incidence of thromboembolic complications was high. Dextran-aspirin and dextran-warfarin appear to be satisfactory and relatively simple methods of thromboembolic prophylaxis.", "PMID": 977621}
{"id": "pubmed23n0032_11210", "title": "A clinical evaluation of aspirin prophylaxis of thromboembolic disease after total hip arthroplasty.", "content": "Prophylaxis of thromboembolic disease using aspirin was studied prospectively in 340 patients undergoing total hip replacement, excluding those with prior thromboembolic disease. Clinical criteria were used to diagnose thrombophlebitis, while clinical criteria, roentgenography, blood-gas determinations, electrocardiography, and pulmonary vascular scanning were used to diagnose pulmonary emboli. Five patients had signs of symptoms of pulmonary emboli, all confirmed by pulmonary vascular scan. No fatal pulmonary emboli occurred. Twenty-six patients had thrombophlebitis. Without simultaneous control group, a reduction in postoperative thromboembolic disease in these patients receiving aspirin was not proved. However, the observed low incidence of clinically evident thromboembolic manifestations suggests that aspirin may be a simple and useful prophylactic agent in these patients.", "contents": "A clinical evaluation of aspirin prophylaxis of thromboembolic disease after total hip arthroplasty. Prophylaxis of thromboembolic disease using aspirin was studied prospectively in 340 patients undergoing total hip replacement, excluding those with prior thromboembolic disease. Clinical criteria were used to diagnose thrombophlebitis, while clinical criteria, roentgenography, blood-gas determinations, electrocardiography, and pulmonary vascular scanning were used to diagnose pulmonary emboli. Five patients had signs of symptoms of pulmonary emboli, all confirmed by pulmonary vascular scan. No fatal pulmonary emboli occurred. Twenty-six patients had thrombophlebitis. Without simultaneous control group, a reduction in postoperative thromboembolic disease in these patients receiving aspirin was not proved. However, the observed low incidence of clinically evident thromboembolic manifestations suggests that aspirin may be a simple and useful prophylactic agent in these patients.", "PMID": 977622}
{"id": "pubmed23n0032_11211", "title": "Thromboembolic disease in patients undergoing total knee replacement.", "content": "In a prospective study fo the incidence of deep-vein thrombosis in thirty patients undergoing total knee replacement, all patients had clinical examinations and 125I fibrinogen scanning, while those suspected of having deep venous thrombosis also had confirmatory venography. Sixteen (53 per cent) of the thirty patients had thromboembolic disease; nine had thrombi only in the limb operated on; four had bilateral deep venous thrombi; and three had pulmonary embolism. In nine patients who took aspirin regularly the incidence of thromboembolism was 11 per cent, while in the eight who did not take aspirin or any other antiplatelet drug the incidence was 88 per cent, a difference which was highly significant (p = 0.003).", "contents": "Thromboembolic disease in patients undergoing total knee replacement. In a prospective study fo the incidence of deep-vein thrombosis in thirty patients undergoing total knee replacement, all patients had clinical examinations and 125I fibrinogen scanning, while those suspected of having deep venous thrombosis also had confirmatory venography. Sixteen (53 per cent) of the thirty patients had thromboembolic disease; nine had thrombi only in the limb operated on; four had bilateral deep venous thrombi; and three had pulmonary embolism. In nine patients who took aspirin regularly the incidence of thromboembolism was 11 per cent, while in the eight who did not take aspirin or any other antiplatelet drug the incidence was 88 per cent, a difference which was highly significant (p = 0.003).", "PMID": 977623}
{"id": "pubmed23n0032_11212", "title": "Venous thrombosis after total hip replacement. Combined monitoring as a guide for prophylaxis and treatment.", "content": "Monitoring of 140 patients after hip-replacement arthroplasty using both impedance plethysmography and 125I fibrinogen scanning of the lower extremities showed that half had no evidence of thrombosis, one-quarter had moderate or extensive thrombosis, and one-quarter had abnormal scans only. In the patients with only abnormal scans the process appeared to resolve spontaneously. The findings by impedance plethysmography therefore appeared to differentiate non-invasively between thromboses that would and would not resolve, and it is suggested that for some patients a monitoring regimen may be preferable to routine prophylaxis.", "contents": "Venous thrombosis after total hip replacement. Combined monitoring as a guide for prophylaxis and treatment. Monitoring of 140 patients after hip-replacement arthroplasty using both impedance plethysmography and 125I fibrinogen scanning of the lower extremities showed that half had no evidence of thrombosis, one-quarter had moderate or extensive thrombosis, and one-quarter had abnormal scans only. In the patients with only abnormal scans the process appeared to resolve spontaneously. The findings by impedance plethysmography therefore appeared to differentiate non-invasively between thromboses that would and would not resolve, and it is suggested that for some patients a monitoring regimen may be preferable to routine prophylaxis.", "PMID": 977624}
{"id": "pubmed23n0032_11213", "title": "Cuff-impedance phlebography and 125I fibrinogen scanning versus roentgenographic phlebography for diagnosis of thrombophlebitis following hip surgery. A preliminary report.", "content": "Seventy-eight limbs of forty-eight patients undergoing total hip replacement were studied by roentgenographic phlebography, cuff-impedance phlebography, and 125I fibrinogen scanning. Compared with roentgenographic phlebography, cuff-impedance phlebography detected seven of ten thrombi in the thigh but none of nine thrombi in the calf and popliteal veins, giving an over-all accuracy of 80 per cent. The 125I fibrinogen scanning technique detected none of the ten thrombi in the thigh and seven of the nine in the calf and popliteal veins, giving an over-all accuracy of 78 per cent. Combining the results of the two techniques, fourteen of the nineteen thrombi were detected. Cuff-impedance phlebography appears to be a useful method for the diagnosis of thrombi in the thigh after hip surgery.", "contents": "Cuff-impedance phlebography and 125I fibrinogen scanning versus roentgenographic phlebography for diagnosis of thrombophlebitis following hip surgery. A preliminary report. Seventy-eight limbs of forty-eight patients undergoing total hip replacement were studied by roentgenographic phlebography, cuff-impedance phlebography, and 125I fibrinogen scanning. Compared with roentgenographic phlebography, cuff-impedance phlebography detected seven of ten thrombi in the thigh but none of nine thrombi in the calf and popliteal veins, giving an over-all accuracy of 80 per cent. The 125I fibrinogen scanning technique detected none of the ten thrombi in the thigh and seven of the nine in the calf and popliteal veins, giving an over-all accuracy of 78 per cent. Combining the results of the two techniques, fourteen of the nineteen thrombi were detected. Cuff-impedance phlebography appears to be a useful method for the diagnosis of thrombi in the thigh after hip surgery.", "PMID": 977625}
{"id": "pubmed23n0032_11214", "title": "Long-term results in the treatment of femoral-shaft fractures in young children by immediate spica immobilization.", "content": "Manual traction reduction without anesthesia and immediate immobilization in a spica cast were used to treat eighty-five children ranging in age from birth to ten years old who had closed femoral-shaft fractures and no associated injuries. Up to two centimeters of overriding, 30 degrees of anterior angulation, and 15 degrees of medial angulation were accepted. Any angulation in excess of these amounts, or lateral or posterior angulation, was corrected by wedging the cast at the fracture site. Further telescoping of the fracture fragments in the case was attributed to the child pressing the foot against the bottom of the cast and was prevented by removing the sole of the cast. Of the seventy-five children examined two to eighteen years after fracture, none had any residual skeletal deformity or joint stiffness. The length discrepancies of the fractured limbs ranged from 1.7 centimeters of shortening to 0.9 centimeter of overgrowth.", "contents": "Long-term results in the treatment of femoral-shaft fractures in young children by immediate spica immobilization. Manual traction reduction without anesthesia and immediate immobilization in a spica cast were used to treat eighty-five children ranging in age from birth to ten years old who had closed femoral-shaft fractures and no associated injuries. Up to two centimeters of overriding, 30 degrees of anterior angulation, and 15 degrees of medial angulation were accepted. Any angulation in excess of these amounts, or lateral or posterior angulation, was corrected by wedging the cast at the fracture site. Further telescoping of the fracture fragments in the case was attributed to the child pressing the foot against the bottom of the cast and was prevented by removing the sole of the cast. Of the seventy-five children examined two to eighteen years after fracture, none had any residual skeletal deformity or joint stiffness. The length discrepancies of the fractured limbs ranged from 1.7 centimeters of shortening to 0.9 centimeter of overgrowth.", "PMID": 977626}
{"id": "pubmed23n0032_11215", "title": "Results of proximal tibial osteotomy. The effects of tibiofemoral angle, stance-phase flexion-extension, and medial-plateau force.", "content": "Forty-eight knees were evaluated after proximal tibial osteotomy, performed for varus deformity to determine the desired amount of correction of the deformity, the effect of osteotomy on knee motion during gait and one medial-plateau force during standing, and the relationships between these factors and the result. Correction of the tibiofemoral angle to 5 degrees of genu valgum or more produced the best and most lasting results. Stance-phase flexion-extension increased the rotation decreased in knees with good results while the other gait parameters were not significantly changed. Medial-plateau force was decreased by successful tibial osteotomy. The knees with the best and most lasting results had 7 degrees of stance-phase flexion-extension or more during walking and either a valgus tibiofemoral angle of 5 degrees or more or a medial-plateau force of 50 per cent of body weight or less.", "contents": "Results of proximal tibial osteotomy. The effects of tibiofemoral angle, stance-phase flexion-extension, and medial-plateau force. Forty-eight knees were evaluated after proximal tibial osteotomy, performed for varus deformity to determine the desired amount of correction of the deformity, the effect of osteotomy on knee motion during gait and one medial-plateau force during standing, and the relationships between these factors and the result. Correction of the tibiofemoral angle to 5 degrees of genu valgum or more produced the best and most lasting results. Stance-phase flexion-extension increased the rotation decreased in knees with good results while the other gait parameters were not significantly changed. Medial-plateau force was decreased by successful tibial osteotomy. The knees with the best and most lasting results had 7 degrees of stance-phase flexion-extension or more during walking and either a valgus tibiofemoral angle of 5 degrees or more or a medial-plateau force of 50 per cent of body weight or less.", "PMID": 977627}
{"id": "pubmed23n0032_11216", "title": "The arterial supply of the developing proximal end of the human femur.", "content": "By means of perfusion studies, an analysis was made of the arterial supply to the proximal end of the femur in 150 specimens from autopsied fetuses and children, aged from twenty-six weeks of gestation to fourteen years and eight months old. All died of diseases which did not involve the hip joint. Two anastomotic rings were found: an extracapsular one formed by the medial and lateral femoral circumflex arteries, and a subsynovial intra-articular ring at the articular cartilage-neck junction. The intra-articular rings in males were discontinuous more often than in females. A three-plane analysis of totally-cleared specimens demonstrated that the epiphyseal plate constituted an absolute barrier to blood flow between the epiphysis and metaphysis in all but two of the 124 barium sulphate-perfused specimens examined. A smaller number of ascending cervical arteries crossed the anterior and medial surfaces of the mid-neck in the specimens from three to ten-year-old white children than in those from newborn to two-year-old white and black children. This finding may be important for the etiology of Legg-Perthes disease. No differences with respect to age, sex, or race were found in the arteries of the ligamentum teres.", "contents": "The arterial supply of the developing proximal end of the human femur. By means of perfusion studies, an analysis was made of the arterial supply to the proximal end of the femur in 150 specimens from autopsied fetuses and children, aged from twenty-six weeks of gestation to fourteen years and eight months old. All died of diseases which did not involve the hip joint. Two anastomotic rings were found: an extracapsular one formed by the medial and lateral femoral circumflex arteries, and a subsynovial intra-articular ring at the articular cartilage-neck junction. The intra-articular rings in males were discontinuous more often than in females. A three-plane analysis of totally-cleared specimens demonstrated that the epiphyseal plate constituted an absolute barrier to blood flow between the epiphysis and metaphysis in all but two of the 124 barium sulphate-perfused specimens examined. A smaller number of ascending cervical arteries crossed the anterior and medial surfaces of the mid-neck in the specimens from three to ten-year-old white children than in those from newborn to two-year-old white and black children. This finding may be important for the etiology of Legg-Perthes disease. No differences with respect to age, sex, or race were found in the arteries of the ligamentum teres.", "PMID": 977628}
{"id": "pubmed23n0032_11217", "title": "Triple tenodesis of the knee. A soft-tissue operation for the correction of paralytic genu recurvatum.", "content": "There are two types of genu recurvatum: one with bone deformity, which responds well to osteotomy, and one with posterior soft-tissue laxity with secondary bone changes. To date, no reliable treatment short of arthrodesis has been effective for the second type. An operation for this type of genu recurvatum (post-poliomyelitic) is described: a soft-tissue reconstruction of the lax tissues posterior to the knee joint done in three layers. The operation was done on sixteen knees in fourteen patients, with an average follow-up of four years and three months. The average recurvatum before surgery was 42 degrees and at the time of follow-up it was 6 degrees. Preliminary surgery is often required and precise surgical technique and prolonged bracing after surgery also are needed. All but one patient was made brace-free provided the limb operated on was not flail.", "contents": "Triple tenodesis of the knee. A soft-tissue operation for the correction of paralytic genu recurvatum. There are two types of genu recurvatum: one with bone deformity, which responds well to osteotomy, and one with posterior soft-tissue laxity with secondary bone changes. To date, no reliable treatment short of arthrodesis has been effective for the second type. An operation for this type of genu recurvatum (post-poliomyelitic) is described: a soft-tissue reconstruction of the lax tissues posterior to the knee joint done in three layers. The operation was done on sixteen knees in fourteen patients, with an average follow-up of four years and three months. The average recurvatum before surgery was 42 degrees and at the time of follow-up it was 6 degrees. Preliminary surgery is often required and precise surgical technique and prolonged bracing after surgery also are needed. All but one patient was made brace-free provided the limb operated on was not flail.", "PMID": 977629}
{"id": "pubmed23n0032_11218", "title": "Gliding function following flexor-tendon injury. A biomechanical study of rat tendon function.", "content": "Gliding function of flexor tendons one hour to eight weeks after a standard injury was studied in rat digits by determining terminal force of flexion, tendon excursion, and work of flexion using a tensile testing machine. A rapid decrease in gliding occurred within hours of injury, indicating that the postoperative hematoma and edema restrict gliding long before collagenous adhesions are synthesized. The improvement in gliding function with time implies that fibrous adhesions can be remodeled. Surgical methods and therapeutic agents, therefore, should be directed toward limiting early tissue injury and seeking ways to enhance the late remodeling of fibrous peritendinous adhesions along functional lines.", "contents": "Gliding function following flexor-tendon injury. A biomechanical study of rat tendon function. Gliding function of flexor tendons one hour to eight weeks after a standard injury was studied in rat digits by determining terminal force of flexion, tendon excursion, and work of flexion using a tensile testing machine. A rapid decrease in gliding occurred within hours of injury, indicating that the postoperative hematoma and edema restrict gliding long before collagenous adhesions are synthesized. The improvement in gliding function with time implies that fibrous adhesions can be remodeled. Surgical methods and therapeutic agents, therefore, should be directed toward limiting early tissue injury and seeking ways to enhance the late remodeling of fibrous peritendinous adhesions along functional lines.", "PMID": 977630}
{"id": "pubmed23n0032_11219", "title": "Ruptures of the tendo achillis. An objective assessment of surgical and non-surgical treatment.", "content": "A comparative study is presented of seventy-nine patients with rupture of the tendo achillis, forty-eight of whom were treated surgically and thirty-one, non-surgically. With one exception, all were healthy, athletic individuals. Twenty-two per cent of the patients had an initial misdiagnosis. The patients treated surgically were more satisfied with the results of their treatment. Strength, power, and endurance as measured on the Cybex II dynamometer revealed that the patients treated non-surgically attained only 72 per cent of normal strength and 70 per cent of normal power and endurance as compared with the surgically treated patients. Wound complications occurred in only two patients and no reruptures occurred in the surgically treated group, whereas there were nine rereptures in the group not treated surgically.", "contents": "Ruptures of the tendo achillis. An objective assessment of surgical and non-surgical treatment. A comparative study is presented of seventy-nine patients with rupture of the tendo achillis, forty-eight of whom were treated surgically and thirty-one, non-surgically. With one exception, all were healthy, athletic individuals. Twenty-two per cent of the patients had an initial misdiagnosis. The patients treated surgically were more satisfied with the results of their treatment. Strength, power, and endurance as measured on the Cybex II dynamometer revealed that the patients treated non-surgically attained only 72 per cent of normal strength and 70 per cent of normal power and endurance as compared with the surgically treated patients. Wound complications occurred in only two patients and no reruptures occurred in the surgically treated group, whereas there were nine rereptures in the group not treated surgically.", "PMID": 977631}
{"id": "pubmed23n0032_11220", "title": "Osteolytic form of Paget's disease. Differential diagnosis and pathogenesis.", "content": "Of fourteen patients with Paget's disease for whom adequate roentgenograms and pathological material were available, six had lesions showing significant or predominant osteolysis. Based on a study of these six patients and a review of the literature, the following mechanisms were identified as causes of the so-called osteolytic type of the disease: (1) an \"early\" destructive Paget's lesion; (2) \"advanced\" Paget's disease with secondary degenerative changes; (3) \"seeding\" of an independent osteolytic lesion, particularly tumor in pre-existing Paget's disease; (4) sarcomatous transformation; and (5) immobilization after fracture. An osteolytic lesion in a patient without other bone disease may be due to Paget's disease, while an associated lytic lesion may be the result of a variety of conditions. Accurate pathological diagnosis is essential.", "contents": "Osteolytic form of Paget's disease. Differential diagnosis and pathogenesis. Of fourteen patients with Paget's disease for whom adequate roentgenograms and pathological material were available, six had lesions showing significant or predominant osteolysis. Based on a study of these six patients and a review of the literature, the following mechanisms were identified as causes of the so-called osteolytic type of the disease: (1) an \"early\" destructive Paget's lesion; (2) \"advanced\" Paget's disease with secondary degenerative changes; (3) \"seeding\" of an independent osteolytic lesion, particularly tumor in pre-existing Paget's disease; (4) sarcomatous transformation; and (5) immobilization after fracture. An osteolytic lesion in a patient without other bone disease may be due to Paget's disease, while an associated lytic lesion may be the result of a variety of conditions. Accurate pathological diagnosis is essential.", "PMID": 977632}
{"id": "pubmed23n0032_11221", "title": "A comparison of computer asisted tomography (EMI scanner) with conventional neuroradiologic methods in the investigation of patients clinically suspected of intracranial tumor.", "content": "Computerized tomography has been used as the initial radiologic investigation among 212 intracranial tumor suspects. Ninety-five of these patients were ultimately shown to have tumors as a result of all their investigations. Eighty tumors were clearly defined by EMI scanning, four further scans were suspicious but not unequivocally abnormal and six scans showed complications of the tumor rather than the lesion itself. Thus, only five scans were falsely negative in the presence of a tumor. The false positive and false negative rates for EMI scanning have been compared with conventional techniques and computerized tomography shown to compare favourably with angiography or encephalography.", "contents": "A comparison of computer asisted tomography (EMI scanner) with conventional neuroradiologic methods in the investigation of patients clinically suspected of intracranial tumor. Computerized tomography has been used as the initial radiologic investigation among 212 intracranial tumor suspects. Ninety-five of these patients were ultimately shown to have tumors as a result of all their investigations. Eighty tumors were clearly defined by EMI scanning, four further scans were suspicious but not unequivocally abnormal and six scans showed complications of the tumor rather than the lesion itself. Thus, only five scans were falsely negative in the presence of a tumor. The false positive and false negative rates for EMI scanning have been compared with conventional techniques and computerized tomography shown to compare favourably with angiography or encephalography.", "PMID": 977636}
{"id": "pubmed23n0032_11222", "title": "Angiographic assessment of pancreatic pseudocyst: a reappraisal.", "content": "The correct diagnosis was established by angiography in 16 of 21 patients with pancreatic pseudocyst. The characteristic angiographic abnormalities are arcuate stretching of intrapancreatic arteries combined with a filling defect in the parenchymal phase of the angiogram. In the five false negative patients, the diagnosis was not made either because of the small size of the pseudocyst or a primary extrapancreatic location of the lesion. Because of this high false negative rate and the accuracy of B-mode ultrasonic scanning in abdominal cystic lesions, ultrasound should be the primary diagnostic modality in patients with suspected pancreatic pseudocysts.", "contents": "Angiographic assessment of pancreatic pseudocyst: a reappraisal. The correct diagnosis was established by angiography in 16 of 21 patients with pancreatic pseudocyst. The characteristic angiographic abnormalities are arcuate stretching of intrapancreatic arteries combined with a filling defect in the parenchymal phase of the angiogram. In the five false negative patients, the diagnosis was not made either because of the small size of the pseudocyst or a primary extrapancreatic location of the lesion. Because of this high false negative rate and the accuracy of B-mode ultrasonic scanning in abdominal cystic lesions, ultrasound should be the primary diagnostic modality in patients with suspected pancreatic pseudocysts.", "PMID": 977638}
{"id": "pubmed23n0032_11223", "title": "M\u00f6nckeberg's arteriosclerosis revisited: or silver vessels among the old.", "content": "This study showed that M\u00f6nckeberg's arteriosclerosis was found in 23 patients over the age of 60. Diabetes or probable diabetes mellitus was found in 22 out of 23 of these patients. It is concluded that roentgenographic demonstration of MAS in this age group is highly specific for diabetes mellitus. Further, a pilot study suggests that if MAS is present, without demonstrable plain film evidence of coexistent IAS, the chances of the patient having elevated cholesterol seems to be approximately one quarter that of the general population over 60. His chances of an elevated triglyceride appear to be about half that of the same population group.", "contents": "M\u00f6nckeberg's arteriosclerosis revisited: or silver vessels among the old. This study showed that M\u00f6nckeberg's arteriosclerosis was found in 23 patients over the age of 60. Diabetes or probable diabetes mellitus was found in 22 out of 23 of these patients. It is concluded that roentgenographic demonstration of MAS in this age group is highly specific for diabetes mellitus. Further, a pilot study suggests that if MAS is present, without demonstrable plain film evidence of coexistent IAS, the chances of the patient having elevated cholesterol seems to be approximately one quarter that of the general population over 60. His chances of an elevated triglyceride appear to be about half that of the same population group.", "PMID": 977640}
{"id": "pubmed23n0032_11224", "title": "Gastric and small bowel polyps in Gardner's syndrome and familial polyposis coli.", "content": "The adenomas of Familial Polyposis Coli and of Gardner's syndrome are widely thought to affect the large bowel only. However, this report and a critical review of the literature shows that of the few hundred reported cases of these diseases, at least thirty-six have also shown gastric and/or small bowel polyps. The majority have been concentrated in stomach, duodenum and ampulla of Vater and the latter two areas have shown the highest incidence of carcinoma. It seems that gastric polyps are especially associated with Familial Polyposis Coli, and duodeno-ampullary ones with Gardner's syndrome.", "contents": "Gastric and small bowel polyps in Gardner's syndrome and familial polyposis coli. The adenomas of Familial Polyposis Coli and of Gardner's syndrome are widely thought to affect the large bowel only. However, this report and a critical review of the literature shows that of the few hundred reported cases of these diseases, at least thirty-six have also shown gastric and/or small bowel polyps. The majority have been concentrated in stomach, duodenum and ampulla of Vater and the latter two areas have shown the highest incidence of carcinoma. It seems that gastric polyps are especially associated with Familial Polyposis Coli, and duodeno-ampullary ones with Gardner's syndrome.", "PMID": 977642}
{"id": "pubmed23n0032_11225", "title": "Structure of the cell envelope of Halobacterium halobium.", "content": "The structure of the isolated cell envelope of Halobacterium halobium is studied by X-ray diffraction, electron microscopy, and biochemical analysis. The envelope consists of the cell membrane and two layers of protein outside. The outer layer of protein shows a regular arrangement of the protein or glycoprotein particles and is therefore identified as the cell wall. Just outside the cell membrane is a 20 A-thick layer of protein. It is a third structure in the envelope, the function of which may be distinct from that of the cell membrane and the cell wall. This inner layer of protein is separated from the outer protein layer by a 65 A-wide space which has an electron density very close to that of the suspending medium, and which can be etched after freeze-fracture. The space is tentatively identified as the periplasmic space. At NaCl concentrations below 2.0 M, both protein layers of the envelope disintegrate. Gel filtration and analytical ultracentrifugation of the soluble components from the two protein layers reveal two major bands of protein with apparent mol wt of approximately 16,000 and 21,000. At the same time, the cell membrane stays essentially intact as long as the Mg++ concentration is kept at treater than or equal to 20 mM. The cell membrane breaks into small fragments when treated with 0.1 M NaCl and EDTA, or with distilled water, and some soluble proteins, including flavins and cytochromes, are released. The cell membrane apparently has an asymmetric core of the lipid bilayer.", "contents": "Structure of the cell envelope of Halobacterium halobium. The structure of the isolated cell envelope of Halobacterium halobium is studied by X-ray diffraction, electron microscopy, and biochemical analysis. The envelope consists of the cell membrane and two layers of protein outside. The outer layer of protein shows a regular arrangement of the protein or glycoprotein particles and is therefore identified as the cell wall. Just outside the cell membrane is a 20 A-thick layer of protein. It is a third structure in the envelope, the function of which may be distinct from that of the cell membrane and the cell wall. This inner layer of protein is separated from the outer protein layer by a 65 A-wide space which has an electron density very close to that of the suspending medium, and which can be etched after freeze-fracture. The space is tentatively identified as the periplasmic space. At NaCl concentrations below 2.0 M, both protein layers of the envelope disintegrate. Gel filtration and analytical ultracentrifugation of the soluble components from the two protein layers reveal two major bands of protein with apparent mol wt of approximately 16,000 and 21,000. At the same time, the cell membrane stays essentially intact as long as the Mg++ concentration is kept at treater than or equal to 20 mM. The cell membrane breaks into small fragments when treated with 0.1 M NaCl and EDTA, or with distilled water, and some soluble proteins, including flavins and cytochromes, are released. The cell membrane apparently has an asymmetric core of the lipid bilayer.", "PMID": 977644}
{"id": "pubmed23n0032_11226", "title": "Environmentally induced changes in mitochondria and endoplasmic reticulum of Saccharomyces carlsbergensis yeast.", "content": "The effects of culture environment on the volume density and surface density of mitochondria and endoplasmic reticulum in a facultative yeast were studied. When compared with cells grown aerobically on a nonrepressive substrate, cells grown in the absence of oxygen showed a sharp reduction in both volume density of mitochondria and surface density of the inner mitochondrial membrane (imm) in the remaining mitochondrial profiles. Use of fermentable (repressive) substrates under aerobic conditions restricted the volume density of mitochondria to a much greater extent than the surface density of imm. The range of mitochondrial volume densities in these experiments was 4-11%. Surface density of endoplasmic reticulum (ER) was sensitive to growth rate and in particular to changes in oxygen tension, showing large fluctuations during both anaerobic and aerobic adaptation. These fluctuations in ER are discussed in relation to the known role of this organelle in lipid metabolism.", "contents": "Environmentally induced changes in mitochondria and endoplasmic reticulum of Saccharomyces carlsbergensis yeast. The effects of culture environment on the volume density and surface density of mitochondria and endoplasmic reticulum in a facultative yeast were studied. When compared with cells grown aerobically on a nonrepressive substrate, cells grown in the absence of oxygen showed a sharp reduction in both volume density of mitochondria and surface density of the inner mitochondrial membrane (imm) in the remaining mitochondrial profiles. Use of fermentable (repressive) substrates under aerobic conditions restricted the volume density of mitochondria to a much greater extent than the surface density of imm. The range of mitochondrial volume densities in these experiments was 4-11%. Surface density of endoplasmic reticulum (ER) was sensitive to growth rate and in particular to changes in oxygen tension, showing large fluctuations during both anaerobic and aerobic adaptation. These fluctuations in ER are discussed in relation to the known role of this organelle in lipid metabolism.", "PMID": 977645}
{"id": "pubmed23n0032_11227", "title": "125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts.", "content": "125I-labeled human epidermal growth factor (hEGF) binds in a specific and saturable manner to human fibroblasts. At 37 degrees C, the cell-bound 125I-hEGF initially may be recovered in a native form by acid extraction; upon subsequent incubation, the cell-bound 125I-hEGF is degraded very rapidly, with the appearance in the medium of 125I-monoiodotyrosine. At 0 degrees C, cell-bound 125I-hEGF is not degraded but slowly dissociates from the cell. The data are consistent with a mechanism in which 125I-hEGF initially is bound to the cell surface and subsequently is internlized before degradation. The degradation is blocked by inhibitors of metabolic energy production (azide, cyanide, dinitrophenol), some protease inhibitors (Tos-Lys-CH2Cl, benzyl guanidobenzoate), a lysosomotropic agent (chloroquine) various local anesthetics (cocaine, lidocaine, procaine), and ammonium chloride. After the binding and degradation of 125I-hEGF the fibroblasts are no longer able to rebind fresh hormone. The binding capacity of these cells is restored by incubation in a serum-containing medium; this restoration is inhibited by cycloheximide or actinomycin D.", "contents": "125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts. 125I-labeled human epidermal growth factor (hEGF) binds in a specific and saturable manner to human fibroblasts. At 37 degrees C, the cell-bound 125I-hEGF initially may be recovered in a native form by acid extraction; upon subsequent incubation, the cell-bound 125I-hEGF is degraded very rapidly, with the appearance in the medium of 125I-monoiodotyrosine. At 0 degrees C, cell-bound 125I-hEGF is not degraded but slowly dissociates from the cell. The data are consistent with a mechanism in which 125I-hEGF initially is bound to the cell surface and subsequently is internlized before degradation. The degradation is blocked by inhibitors of metabolic energy production (azide, cyanide, dinitrophenol), some protease inhibitors (Tos-Lys-CH2Cl, benzyl guanidobenzoate), a lysosomotropic agent (chloroquine) various local anesthetics (cocaine, lidocaine, procaine), and ammonium chloride. After the binding and degradation of 125I-hEGF the fibroblasts are no longer able to rebind fresh hormone. The binding capacity of these cells is restored by incubation in a serum-containing medium; this restoration is inhibited by cycloheximide or actinomycin D.", "PMID": 977646}
{"id": "pubmed23n0032_11228", "title": "Involvement of bristle coat structures in surface membrane formations and membrane interactions during coenocytotomic cleavage in caps of Acetabularia mediterranea.", "content": "In the multinucleate cap rays of the green alga Acetabularia mediterranea the cell surface increases dramatically within a short time period during the final stages of coenocytotomic cleavage. In early stages of cyst formation the cytoplast is traversed by numerous large and prolate cleavage vesicles which are characterized by typical columellar or spinous coat structures. The cleavage vesicles are closely associated with the surface of plastids and, to a lesser degree, of mitochondria. This intimate association seems to be mediated by regularly spaced, densely stained intermembranous cross-bridge structures and is maintained throughout cleavage. These cleavage vesicles contain a finely fibrillar material structurally similar to the hyaline layer of mucilage that fills the space between the plasma membrane and cell wall. They line up with invaginations of the plasmalemma and vacuole membranes and, together with smaller vesicles interspersed, constitute preformed \"perforation lines\" for the final separation of the coenoblast portions. Equidistantly spaced plaques of attachment of such vesicles with surface membrane are described. We hypothesize (a) that the cleavage vesicle membrane is the immediate precursor to the new postcoenocytotomic surface membrane, (b) that the cleavage vesicle coat structures are integrated into the subsurface coat of the plasma membrane, (c) that growth of the laterally attached cleavage vesicles by intussusception of small fuzzy-coated vesicles is confined to their \"free ends,\" (d) that the intermembranous cross-bridge elements are related to bristle coat structures and play a role in the establishment of the cleavage lines, and (e) that the coenocytotomic cleavage process is organized so that adjacent plastids are separated in a way that guarantees the inclusion of several plastids in each cyst.", "contents": "Involvement of bristle coat structures in surface membrane formations and membrane interactions during coenocytotomic cleavage in caps of Acetabularia mediterranea. In the multinucleate cap rays of the green alga Acetabularia mediterranea the cell surface increases dramatically within a short time period during the final stages of coenocytotomic cleavage. In early stages of cyst formation the cytoplast is traversed by numerous large and prolate cleavage vesicles which are characterized by typical columellar or spinous coat structures. The cleavage vesicles are closely associated with the surface of plastids and, to a lesser degree, of mitochondria. This intimate association seems to be mediated by regularly spaced, densely stained intermembranous cross-bridge structures and is maintained throughout cleavage. These cleavage vesicles contain a finely fibrillar material structurally similar to the hyaline layer of mucilage that fills the space between the plasma membrane and cell wall. They line up with invaginations of the plasmalemma and vacuole membranes and, together with smaller vesicles interspersed, constitute preformed \"perforation lines\" for the final separation of the coenoblast portions. Equidistantly spaced plaques of attachment of such vesicles with surface membrane are described. We hypothesize (a) that the cleavage vesicle membrane is the immediate precursor to the new postcoenocytotomic surface membrane, (b) that the cleavage vesicle coat structures are integrated into the subsurface coat of the plasma membrane, (c) that growth of the laterally attached cleavage vesicles by intussusception of small fuzzy-coated vesicles is confined to their \"free ends,\" (d) that the intermembranous cross-bridge elements are related to bristle coat structures and play a role in the establishment of the cleavage lines, and (e) that the coenocytotomic cleavage process is organized so that adjacent plastids are separated in a way that guarantees the inclusion of several plastids in each cyst.", "PMID": 977647}
{"id": "pubmed23n0032_11229", "title": "Variations in distribution of con A receptor sites and anionic groups during red blood cell differentiation in the rat.", "content": "The distribution of receptors for concanavalin A (Con A) and anionic groups on plasma membranes of developing blood cells was investigated in the rat. Glutaraldehyde-fixed bone marrow and circulating blood cells were exposed to ferritin-conjugated Con A or positively chaged ferric oxide (CI) and processed for electro n microscopy. The frequency of Con A and CI binding sites varied during different erythroid developmental stages and amont different leukoid cell types. There was a constant inverse relationship between Con A and CI binding sites. Among leukoid cells, Con A binding was high on plasma cells and macrophages, lower on neutrophils and lymphocytes, and still lower on eosinophils and basophils; CI binding was highest in the latter and lowest in plasma cells and macrophages. Among erythroid cells, there was a progressive increase in Con A and a decrease in CI binding after successive divisions of erythroblasts, and a progressive decrease in Con A and an increase in CI binding upon maturation of the orthochromatic erythroblast to the reticulocyte. The most pronounced modification in distribution of these sites occurred during nuclear expulsion: that protion of the plasma membrane surrounding the extruded nucleus was heavily labeled by Con A (up to four times that of the orthochromatic erythroblast) whereas the reticulocyte had considerably fewer sites. The situation was reversed with CI. The results suggest that the concentration of nonsialated glycoproteins (to which Con A binds) varies inversely to that of sialoproteins (to which CI binds) in the membrane of the differentiating erythroid cell. The remarkable changes observed at the time of nuclear extrusion suggest that there is either local modification of glycosylated groups with removal of sialyl residues from the membrane surrounding the extruded nucleus of selective segregation of membrane glycoproteins leading to a high concentration of sialoproteins (glycophroin) in the membrane of the mature erythrocyte.", "contents": "Variations in distribution of con A receptor sites and anionic groups during red blood cell differentiation in the rat. The distribution of receptors for concanavalin A (Con A) and anionic groups on plasma membranes of developing blood cells was investigated in the rat. Glutaraldehyde-fixed bone marrow and circulating blood cells were exposed to ferritin-conjugated Con A or positively chaged ferric oxide (CI) and processed for electro n microscopy. The frequency of Con A and CI binding sites varied during different erythroid developmental stages and amont different leukoid cell types. There was a constant inverse relationship between Con A and CI binding sites. Among leukoid cells, Con A binding was high on plasma cells and macrophages, lower on neutrophils and lymphocytes, and still lower on eosinophils and basophils; CI binding was highest in the latter and lowest in plasma cells and macrophages. Among erythroid cells, there was a progressive increase in Con A and a decrease in CI binding after successive divisions of erythroblasts, and a progressive decrease in Con A and an increase in CI binding upon maturation of the orthochromatic erythroblast to the reticulocyte. The most pronounced modification in distribution of these sites occurred during nuclear expulsion: that protion of the plasma membrane surrounding the extruded nucleus was heavily labeled by Con A (up to four times that of the orthochromatic erythroblast) whereas the reticulocyte had considerably fewer sites. The situation was reversed with CI. The results suggest that the concentration of nonsialated glycoproteins (to which Con A binds) varies inversely to that of sialoproteins (to which CI binds) in the membrane of the differentiating erythroid cell. The remarkable changes observed at the time of nuclear extrusion suggest that there is either local modification of glycosylated groups with removal of sialyl residues from the membrane surrounding the extruded nucleus of selective segregation of membrane glycoproteins leading to a high concentration of sialoproteins (glycophroin) in the membrane of the mature erythrocyte.", "PMID": 977648}
{"id": "pubmed23n0032_11230", "title": "Redistribution of surface anionic sites on the luminal front of blood vessel endothelium after interaction with polycationic ligand.", "content": "The ability of anionic groups on the luminal surface of blood vessels to redistribute by lateral migration under the influence of multivalent ligands was analyzed by electron microscopy, using cationized ferritin (CF). In vitro interaction of blood vessel segments with CF results in rapid aggregation of most anionic sites on the luminal fromt of the endothelium, followed by internalization or detachment of the CF patches, leaving most of the luminal surface devoid of anionic sites. Further incubation of such endothelial cells without CF results in regeneration of binding capacity for the polycationic label. Transport of CF, but not of native ferritin, across the endothelium by vesicle transport, followed by exocytosis of the interiorized CF clusters on the tissue front of the endothelium, was also observed. The possibility that such activities in the blood vessels in vivo may be associated with local changes in the normal distribution of the surface anionic sites as well as in accumulation of debris in the subendothelial layers of the vessels is suggested.", "contents": "Redistribution of surface anionic sites on the luminal front of blood vessel endothelium after interaction with polycationic ligand. The ability of anionic groups on the luminal surface of blood vessels to redistribute by lateral migration under the influence of multivalent ligands was analyzed by electron microscopy, using cationized ferritin (CF). In vitro interaction of blood vessel segments with CF results in rapid aggregation of most anionic sites on the luminal fromt of the endothelium, followed by internalization or detachment of the CF patches, leaving most of the luminal surface devoid of anionic sites. Further incubation of such endothelial cells without CF results in regeneration of binding capacity for the polycationic label. Transport of CF, but not of native ferritin, across the endothelium by vesicle transport, followed by exocytosis of the interiorized CF clusters on the tissue front of the endothelium, was also observed. The possibility that such activities in the blood vessels in vivo may be associated with local changes in the normal distribution of the surface anionic sites as well as in accumulation of debris in the subendothelial layers of the vessels is suggested.", "PMID": 977649}
{"id": "pubmed23n0032_11231", "title": "Paramyosin in invertebrate muscles. II. Content in relation to structure and function.", "content": "By quantitative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, paramyosin:myosin heavy chain molecular ratios were calculated for three molluscan muscles:Aequipecten striated adductor, Mercenaria opaque adductor, and Mytilus anterior byssus retractor; and four arthropodan muscles:Limulus telson, Homarus slow claw. Balanus scutal depressor, and Lethocerus air tube retractor. These ratios correlate positively with both thick filament dimensions and maximum active tension development in these tissues. The role of paramyosin in these muscles is discussed with respect to the following characteristics: force development, \"catch,\" and extreme reversible changes in length.", "contents": "Paramyosin in invertebrate muscles. II. Content in relation to structure and function. By quantitative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, paramyosin:myosin heavy chain molecular ratios were calculated for three molluscan muscles:Aequipecten striated adductor, Mercenaria opaque adductor, and Mytilus anterior byssus retractor; and four arthropodan muscles:Limulus telson, Homarus slow claw. Balanus scutal depressor, and Lethocerus air tube retractor. These ratios correlate positively with both thick filament dimensions and maximum active tension development in these tissues. The role of paramyosin in these muscles is discussed with respect to the following characteristics: force development, \"catch,\" and extreme reversible changes in length.", "PMID": 977650}
{"id": "pubmed23n0032_11232", "title": "Relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan.", "content": "Mouse 3T3 cells and their Simian Virus 40-transformed derivatives (3T3SV) were used to assess the relationship of transfromation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan (GAG). Glucosamine- and galactosamine-containing GAG were labeled equivalently by [3H=A1-glucose regardless of culture type, allowing incorporation into the various GAG to be compared under all conditions studied. Three components of each culture type were examined: the cells, which contain the bulk of newly synthesized GAG and are enriched in chondroitin sulfate and heparan sulfate; cell surface materials released by trypsin, which contain predominantly hyaluronic acid; and the media , which contain predominantly hyaluronic acid and undersulfated chondroitin sulfate. Increased cell density and viral transformation reduce incorporation into GAG relative to the incorporation into other polysaccharides. Transformation, however, does not substantially alter the type or distribution of newly synthesized GAG; the relative amounts and cellular distributions were very similar in 3T3 and 3T3SV cultures growing at similar rates at low densities. On the other hand, increased cell density as well as density-dependent growth inhibition modified the type and distribution of newly synthesized GAG. At high cell densities both cell types showed reduced incorporation into hyaluronate and an increase in cellular GAG due to enhanced labeling of chondroitin sulfate and heparan sulfate. These changes were more marked in confluent 3T3 cultures which also differed in showing substantially more GAG label in the medium and in chondroitin-6-sulfate and heparan sulfate at the cell surface. Since cell density and possibly density-dependent inhibition of growth but not viral transformation are major factors controlling the cellular distribution and type of newly synthesized GAG, differences due to GAG's in the culture behavior of normal and transformed cells may occur only at high cell density. The density-induced GAG alterations most likely involved are increased condroitin-6-sulfate and heparan sulfate and decreased hyaluronic acid at the cell surface.", "contents": "Relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan. Mouse 3T3 cells and their Simian Virus 40-transformed derivatives (3T3SV) were used to assess the relationship of transfromation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan (GAG). Glucosamine- and galactosamine-containing GAG were labeled equivalently by [3H=A1-glucose regardless of culture type, allowing incorporation into the various GAG to be compared under all conditions studied. Three components of each culture type were examined: the cells, which contain the bulk of newly synthesized GAG and are enriched in chondroitin sulfate and heparan sulfate; cell surface materials released by trypsin, which contain predominantly hyaluronic acid; and the media , which contain predominantly hyaluronic acid and undersulfated chondroitin sulfate. Increased cell density and viral transformation reduce incorporation into GAG relative to the incorporation into other polysaccharides. Transformation, however, does not substantially alter the type or distribution of newly synthesized GAG; the relative amounts and cellular distributions were very similar in 3T3 and 3T3SV cultures growing at similar rates at low densities. On the other hand, increased cell density as well as density-dependent growth inhibition modified the type and distribution of newly synthesized GAG. At high cell densities both cell types showed reduced incorporation into hyaluronate and an increase in cellular GAG due to enhanced labeling of chondroitin sulfate and heparan sulfate. These changes were more marked in confluent 3T3 cultures which also differed in showing substantially more GAG label in the medium and in chondroitin-6-sulfate and heparan sulfate at the cell surface. Since cell density and possibly density-dependent inhibition of growth but not viral transformation are major factors controlling the cellular distribution and type of newly synthesized GAG, differences due to GAG's in the culture behavior of normal and transformed cells may occur only at high cell density. The density-induced GAG alterations most likely involved are increased condroitin-6-sulfate and heparan sulfate and decreased hyaluronic acid at the cell surface.", "PMID": 977651}
{"id": "pubmed23n0032_11233", "title": "Cytochalasin B inhibits actin-related gelation of HeLa cell extracts.", "content": "When the 100,000 g supernatant fraction (extract) of HeLa cells lysed in a buffer containing sucrose, ATP, DTE, EGTA, imidazole, and Triton X-100 is incubated at 25 degrees C, it gels, and actin and a HMWP are progressively enriched in the extract and in gel isolated from extract. CB (greater than or equal to 0.25 muM) inhibits gelation and specifically lowers the concentrations of actin and the HMWP in the fraction which sediments at 100,000 g after incubation. These results indicate that actin and HMWP are partly disaggregated by cytochalasin treatment, and thus that their aggregation is related gelation. Inasmuch as previous results showed that actin is present and HMWP is enriched in the plasma membrane fraction of HeLa cells, the results also point to a possible relation between plasma membrane-associated gel and in vivo effects of CB.", "contents": "Cytochalasin B inhibits actin-related gelation of HeLa cell extracts. When the 100,000 g supernatant fraction (extract) of HeLa cells lysed in a buffer containing sucrose, ATP, DTE, EGTA, imidazole, and Triton X-100 is incubated at 25 degrees C, it gels, and actin and a HMWP are progressively enriched in the extract and in gel isolated from extract. CB (greater than or equal to 0.25 muM) inhibits gelation and specifically lowers the concentrations of actin and the HMWP in the fraction which sediments at 100,000 g after incubation. These results indicate that actin and HMWP are partly disaggregated by cytochalasin treatment, and thus that their aggregation is related gelation. Inasmuch as previous results showed that actin is present and HMWP is enriched in the plasma membrane fraction of HeLa cells, the results also point to a possible relation between plasma membrane-associated gel and in vivo effects of CB.", "PMID": 977652}
{"id": "pubmed23n0032_11234", "title": "Absence of rDNA amplification in the uninucleolate oocyte of the cockroach Blattella germanica (Oorthoptera: Blattidae).", "content": "Amplification of the genes coding for ribosomal RNA oocurs in the oocytes of a wide variety of organisms. In oocytes of various species of crickets (Orthoptera: Gryllidae) the amplified DNA is contained in a large extrachromosomal DNA body. Multiple nucleoli form about the periphery of the DNA body during the diplotene stage of meiosis I. In contrast to the general pattern of orthopteran oocytes, oocytes of the cockroach Blattella germanica demonstrate a single large nucleolus instead of many nucleoli. In order to determine whether the genes coding for rRNA are amplified in the oocytes of B. germanica, the relative amount of rDNA in oocytes was compared with the rDNA content of spermatocytes and somatic cells. An extrachromosomal DNA body similar to that present in crickets is not present in B. germanica. A satellite DNA band which contains nucleotide sequences complementary to rRNA accounts for approximately 3-5% of the total DNA in somatic and in male and female gametogenic tissues. Female cells contain approximately twice as much rDNA as do male cells. An XX-XO sex-determining mechanism is operative in B. germanica. In situ hybridization with rRNA indicates that the nucleolar organizer is located on one end of the X chromosome and that oocytes do not contain more than twice the amount of rDNA found in spermato cytes. The data indicate that rDNA is not amplified in the uninucleolate oocyte of B germanica.", "contents": "Absence of rDNA amplification in the uninucleolate oocyte of the cockroach Blattella germanica (Oorthoptera: Blattidae). Amplification of the genes coding for ribosomal RNA oocurs in the oocytes of a wide variety of organisms. In oocytes of various species of crickets (Orthoptera: Gryllidae) the amplified DNA is contained in a large extrachromosomal DNA body. Multiple nucleoli form about the periphery of the DNA body during the diplotene stage of meiosis I. In contrast to the general pattern of orthopteran oocytes, oocytes of the cockroach Blattella germanica demonstrate a single large nucleolus instead of many nucleoli. In order to determine whether the genes coding for rRNA are amplified in the oocytes of B. germanica, the relative amount of rDNA in oocytes was compared with the rDNA content of spermatocytes and somatic cells. An extrachromosomal DNA body similar to that present in crickets is not present in B. germanica. A satellite DNA band which contains nucleotide sequences complementary to rRNA accounts for approximately 3-5% of the total DNA in somatic and in male and female gametogenic tissues. Female cells contain approximately twice as much rDNA as do male cells. An XX-XO sex-determining mechanism is operative in B. germanica. In situ hybridization with rRNA indicates that the nucleolar organizer is located on one end of the X chromosome and that oocytes do not contain more than twice the amount of rDNA found in spermato cytes. The data indicate that rDNA is not amplified in the uninucleolate oocyte of B germanica.", "PMID": 977654}
{"id": "pubmed23n0032_11235", "title": "Vitreous body collagen. Evidence for a dual origin from the neural retina and hyalocytes.", "content": "Two different cells types have been shown to synthesize embryonic chick vitreous collagen (vitrosin) at different stages of development. Identification of vitrosin was established by labeling the embryos in ovo [3H]proline at stages 23 and 28 and separating the extracted vitreous collagen alpha-chains by carboxymethylcellulose chromatography. The labeled collagen consisted predominately of alpha 1 chains, indicating a molecule in the form of a trimer of identical chains designated (alpha 1)3. The molecular weight of the labeled chains measured approximately 95,000 daltons by molecular sieve chromatography, and contained 41% of their imino acid as 4-hydroxyproline. To establish which eye tissues synthesize vitrosine, the collagens produced in organ culture by the isolated neural retina, lens and vitreous body from stages 26-27, 29-30, and 40 were examined. At the two earlier stages, only the neural retina synthesized large quantities of (alpha 1)3 collagen whereas the lens and the cells within the vitreous body itself synthesized relatively small amounts of collagen characterized by an alpha 1:alpha 2 ratio of about 2:1. At stage 40, however, the cells of the vitreous body itself synthesized the greatest quantities of collagen, which now was predominantly an (alpha 1)3 type molecule. Stage 40 neural retina and lens synthesized lesser amounts of collagen with an alpha 1:alpha 2 ratio of 2 to 3:1. Chick vitrosin thus appears to be synthesized by the neural retina in early embryonic stages, whereas the major contribution derives from cells within the vitreous body in later development.", "contents": "Vitreous body collagen. Evidence for a dual origin from the neural retina and hyalocytes. Two different cells types have been shown to synthesize embryonic chick vitreous collagen (vitrosin) at different stages of development. Identification of vitrosin was established by labeling the embryos in ovo [3H]proline at stages 23 and 28 and separating the extracted vitreous collagen alpha-chains by carboxymethylcellulose chromatography. The labeled collagen consisted predominately of alpha 1 chains, indicating a molecule in the form of a trimer of identical chains designated (alpha 1)3. The molecular weight of the labeled chains measured approximately 95,000 daltons by molecular sieve chromatography, and contained 41% of their imino acid as 4-hydroxyproline. To establish which eye tissues synthesize vitrosine, the collagens produced in organ culture by the isolated neural retina, lens and vitreous body from stages 26-27, 29-30, and 40 were examined. At the two earlier stages, only the neural retina synthesized large quantities of (alpha 1)3 collagen whereas the lens and the cells within the vitreous body itself synthesized relatively small amounts of collagen characterized by an alpha 1:alpha 2 ratio of about 2:1. At stage 40, however, the cells of the vitreous body itself synthesized the greatest quantities of collagen, which now was predominantly an (alpha 1)3 type molecule. Stage 40 neural retina and lens synthesized lesser amounts of collagen with an alpha 1:alpha 2 ratio of 2 to 3:1. Chick vitrosin thus appears to be synthesized by the neural retina in early embryonic stages, whereas the major contribution derives from cells within the vitreous body in later development.", "PMID": 977655}
{"id": "pubmed23n0032_11236", "title": "Intercellular adhesive selectivity. III. Species selectivity of embryonic liver intercellular adhesion.", "content": "A species difference in the intercellular adhesive selectivity of mixtures of embryonic liver cells is reported. This is first quantitative assessment of species differences in the intercellular adhesive properties of embryonic cells. A collecting aggregate assay, a new double-label assay procedure, and histological and autoradiographic procedures were used to elucidate the intercellular adhesive selectivity of developing mammalian and avian liver cells. Evidence is presented that the reported adhesive differences are not due to the different cell types composing the respective embryonic mammalian and avian livers. Finally, such heterolgous-homotypic selectivity of adhesion is not a property of all tissues, since it is shown that developing brain cells (mesencephalon) do not exhibit the avove intercellular adhesive selectivity (mammalian vs. avian). These findings provide further support for the hypothesis that generic identity as well as cell type may play an important part in determining the intercellular adhesive behavior of heterologous-homotypic mixtures of embryonic cells. A possible evolutionary divergence of morphogenetic mechanisms is discussed.", "contents": "Intercellular adhesive selectivity. III. Species selectivity of embryonic liver intercellular adhesion. A species difference in the intercellular adhesive selectivity of mixtures of embryonic liver cells is reported. This is first quantitative assessment of species differences in the intercellular adhesive properties of embryonic cells. A collecting aggregate assay, a new double-label assay procedure, and histological and autoradiographic procedures were used to elucidate the intercellular adhesive selectivity of developing mammalian and avian liver cells. Evidence is presented that the reported adhesive differences are not due to the different cell types composing the respective embryonic mammalian and avian livers. Finally, such heterolgous-homotypic selectivity of adhesion is not a property of all tissues, since it is shown that developing brain cells (mesencephalon) do not exhibit the avove intercellular adhesive selectivity (mammalian vs. avian). These findings provide further support for the hypothesis that generic identity as well as cell type may play an important part in determining the intercellular adhesive behavior of heterologous-homotypic mixtures of embryonic cells. A possible evolutionary divergence of morphogenetic mechanisms is discussed.", "PMID": 977656}
{"id": "pubmed23n0032_11237", "title": "Interrelationships between water and cellular metabolism in Artemia cysts. V. 14CO2 incorporation.", "content": "The ability of cysts of the brine shrimp, Artemia salina, to incorporate 14CO2 into organic compounds soluble in cold-trichloroacetic acid was examined over a broad range of cellular water concentrations. Carbon dioxide was not incorporated by cysts containing less than about 0.3 g H2O/g dried cysts, the \"critical hydration\" for CO2-fixation. This relationship held whether the cysts were hydrated from the liquid or the vapor phase. The incorporation of radioactivity was shown to be due exclusively to metabolic activity in the cellular component of the cyst. Above the critical hydration, the amount of 14CO2 incorporated was a function of cyst water content, but the kinds of metabolites labelled with this precursor, and their relative proportions, were found to be similar in cysts of greatly different hydration. Almost all of the radioactivity was associated with amino acids, Krebs cycle intermediates and related acids, and pyrimidine nucleotides. The fact that the pathway involved with CO2-fixation, and subsequent metabolism of the fixation products are all initiated in cysts containing as little as 0.3 g H2O/g is particularly noteworthy since this hydration level is well within the range of the amounts of \"bound water\" described in the literature for a wide array of cells and tissues.", "contents": "Interrelationships between water and cellular metabolism in Artemia cysts. V. 14CO2 incorporation. The ability of cysts of the brine shrimp, Artemia salina, to incorporate 14CO2 into organic compounds soluble in cold-trichloroacetic acid was examined over a broad range of cellular water concentrations. Carbon dioxide was not incorporated by cysts containing less than about 0.3 g H2O/g dried cysts, the \"critical hydration\" for CO2-fixation. This relationship held whether the cysts were hydrated from the liquid or the vapor phase. The incorporation of radioactivity was shown to be due exclusively to metabolic activity in the cellular component of the cyst. Above the critical hydration, the amount of 14CO2 incorporated was a function of cyst water content, but the kinds of metabolites labelled with this precursor, and their relative proportions, were found to be similar in cysts of greatly different hydration. Almost all of the radioactivity was associated with amino acids, Krebs cycle intermediates and related acids, and pyrimidine nucleotides. The fact that the pathway involved with CO2-fixation, and subsequent metabolism of the fixation products are all initiated in cysts containing as little as 0.3 g H2O/g is particularly noteworthy since this hydration level is well within the range of the amounts of \"bound water\" described in the literature for a wide array of cells and tissues.", "PMID": 977657}
{"id": "pubmed23n0032_11238", "title": "Endotoxin-induced size change in bone marrow progenitors of granulocytes and macrophages.", "content": "Injection of 5 mug endotoxin to adult C57BL mice caused a marked increase in the sedimentation velocity of granulocytic and macrophage progenitor (colony-forming) cells in the bone marrow. This change was maximal two days after injection and was not accompanied by corresponding changes in total marrow nucleated cell populations. The endotoxin-induced shift was not dependent on the presence of the thymus but did not occur in mice challenged after preinjection with endotoxin. No changes in buoyant density, cell cycle status, pattern of differentiation and responsiveness of granulocytic and macrophage progenitor cells were observed after the injection of endotoxin. The increased sedimentation velocity of progenitor cells appears to indicate an increase in cell volume but the mechanisms involved have not been identified.", "contents": "Endotoxin-induced size change in bone marrow progenitors of granulocytes and macrophages. Injection of 5 mug endotoxin to adult C57BL mice caused a marked increase in the sedimentation velocity of granulocytic and macrophage progenitor (colony-forming) cells in the bone marrow. This change was maximal two days after injection and was not accompanied by corresponding changes in total marrow nucleated cell populations. The endotoxin-induced shift was not dependent on the presence of the thymus but did not occur in mice challenged after preinjection with endotoxin. No changes in buoyant density, cell cycle status, pattern of differentiation and responsiveness of granulocytic and macrophage progenitor cells were observed after the injection of endotoxin. The increased sedimentation velocity of progenitor cells appears to indicate an increase in cell volume but the mechanisms involved have not been identified.", "PMID": 977658}
{"id": "pubmed23n0032_11239", "title": "Stimulation of DNA synthesis, cell multiplication, and ornithine decarboxylase in 3T3 cells by multiplication stimulating activity (MSA).", "content": "Multiplication stimulating activity (MSA) has been purified from the conditioned media of rat liver cells in culture by a modification of the procedure of Dulak and Temin. Purified MSA stimulates [3H] thymidine incorporation into DNA in subconfluent, serum starved 3T3 cells. Cell cycle analysis by the flow microfluorometer shows that the [3H] thymidine incorporation data reflects DNA synthesis. MSA also stimulates the multiplication of serum starved subconfluent 3T3 cells. MSA is approximately 10-fold less active in 3T3 cells than in chick embryo fibroblasts in stimulating [3H] thymiding incorporation into DNA. MSA causes a 2--10-fold increase in ornithine decarboxylase (ODC) activity in 3T3 cells and the dose response curve parallels the dose response curve for [3H] thymidine incorporation into DNA. The Km of ODC for ornithine is 0.12 mM. There is a 30% decrease in the activity of ornithine transaminase (OTA) during the time period in which MSA causes an increase in ODC activity. Insulin also stimulates [3H] thymidine incorporation into DNA, cell multiplication and ODC activity over the same concentration range as shown for MSA, however, the extent of stimulation by insulin is less than that observed following MSA addition.", "contents": "Stimulation of DNA synthesis, cell multiplication, and ornithine decarboxylase in 3T3 cells by multiplication stimulating activity (MSA). Multiplication stimulating activity (MSA) has been purified from the conditioned media of rat liver cells in culture by a modification of the procedure of Dulak and Temin. Purified MSA stimulates [3H] thymidine incorporation into DNA in subconfluent, serum starved 3T3 cells. Cell cycle analysis by the flow microfluorometer shows that the [3H] thymidine incorporation data reflects DNA synthesis. MSA also stimulates the multiplication of serum starved subconfluent 3T3 cells. MSA is approximately 10-fold less active in 3T3 cells than in chick embryo fibroblasts in stimulating [3H] thymiding incorporation into DNA. MSA causes a 2--10-fold increase in ornithine decarboxylase (ODC) activity in 3T3 cells and the dose response curve parallels the dose response curve for [3H] thymidine incorporation into DNA. The Km of ODC for ornithine is 0.12 mM. There is a 30% decrease in the activity of ornithine transaminase (OTA) during the time period in which MSA causes an increase in ODC activity. Insulin also stimulates [3H] thymidine incorporation into DNA, cell multiplication and ODC activity over the same concentration range as shown for MSA, however, the extent of stimulation by insulin is less than that observed following MSA addition.", "PMID": 977659}
{"id": "pubmed23n0032_11240", "title": "Potassium transport in normal and transformed mouse 3T3 cells.", "content": "The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in expontntial and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent inhibition of growth (4 X 10(4) to 4 X 10(5) cell cm-2) whereas the SV40 3T3 maintains exponential or near exponential growth (4 X 10(4) to 1 X 10(6) cell cm-2). In agreement with previous observations, volume per cell and mg protein per cell decrease with increasing cell density. Thus, transport measurements have been expressed on a per volume basis. Total unidirectional K influx and efflux in the 3T3 cell is approximately double that of the SV40 3T3 cell at all cell densities investigated. Both cell types have similar volumes initially and show similar decreases with increasing cell density. Thus, in this clone of the 3T3 cell SV40 transformation specifically decreases unidirectional K flux. The magnitude of the total K flux does not change substantially for either cell line during transition from sparse to dense cultures. However, the components of the K transport undergo distinct changes. Both cell lines possess a ouabain sensitive component of K influx, presumably representing the active inward K pump. Both also possess components of K influx and efflux sensitive to furosemide. The data suggest this component represents a one-for-one K exchange mechanism. The fraction of K influx mediated by the ouabain sensitive component is reduced to one half its value when exponential versus density inhibited 3T3 cells are compared (63% versus 31% of total influx). No comparable drop occurs in the SV40 3T3 cell at equivalent cell densities (64% versus 56% of total influx). Thus, the pump mediated component of K influx would appear to be correlated with growth. In contrast, the furosemide sensitive component represents approximately 20% of the total unidirectional K influx and efflux in both cell lines in sparse culture. At high cell densities, where growth inhibition occurs in the 3T3 cell but not the SV40 3T3, the furosemide sensitive component doubles in both cell lines. Thus, the apparent K-K exchange mechanism is density dependent rather than growth dependent.", "contents": "Potassium transport in normal and transformed mouse 3T3 cells. The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in expontntial and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent inhibition of growth (4 X 10(4) to 4 X 10(5) cell cm-2) whereas the SV40 3T3 maintains exponential or near exponential growth (4 X 10(4) to 1 X 10(6) cell cm-2). In agreement with previous observations, volume per cell and mg protein per cell decrease with increasing cell density. Thus, transport measurements have been expressed on a per volume basis. Total unidirectional K influx and efflux in the 3T3 cell is approximately double that of the SV40 3T3 cell at all cell densities investigated. Both cell types have similar volumes initially and show similar decreases with increasing cell density. Thus, in this clone of the 3T3 cell SV40 transformation specifically decreases unidirectional K flux. The magnitude of the total K flux does not change substantially for either cell line during transition from sparse to dense cultures. However, the components of the K transport undergo distinct changes. Both cell lines possess a ouabain sensitive component of K influx, presumably representing the active inward K pump. Both also possess components of K influx and efflux sensitive to furosemide. The data suggest this component represents a one-for-one K exchange mechanism. The fraction of K influx mediated by the ouabain sensitive component is reduced to one half its value when exponential versus density inhibited 3T3 cells are compared (63% versus 31% of total influx). No comparable drop occurs in the SV40 3T3 cell at equivalent cell densities (64% versus 56% of total influx). Thus, the pump mediated component of K influx would appear to be correlated with growth. In contrast, the furosemide sensitive component represents approximately 20% of the total unidirectional K influx and efflux in both cell lines in sparse culture. At high cell densities, where growth inhibition occurs in the 3T3 cell but not the SV40 3T3, the furosemide sensitive component doubles in both cell lines. Thus, the apparent K-K exchange mechanism is density dependent rather than growth dependent.", "PMID": 977660}
{"id": "pubmed23n0032_11241", "title": "Growth of cultured cells from patients with ataxia-telangiectasia.", "content": "Fibroblast cultures derived from skin biopsies of patients with ataxia-telangiectasia had doubling times (mean of five lines: 29.9 +/- 0.6 hours) significantly longer than normal controls (mean of ten lines: 22.4 +/- 0.5 hours).", "contents": "Growth of cultured cells from patients with ataxia-telangiectasia. Fibroblast cultures derived from skin biopsies of patients with ataxia-telangiectasia had doubling times (mean of five lines: 29.9 +/- 0.6 hours) significantly longer than normal controls (mean of ten lines: 22.4 +/- 0.5 hours).", "PMID": 977661}
{"id": "pubmed23n0032_11242", "title": "Alpha-aminoisobutyric acid transport into human glia and glioma cells in culture.", "content": "The AIB transport into human glia and glioma cells in culture has been studied. Because of the high affinity of AIB to the plastic culture dishes, a special washing technique had to be developed. With this technique, it was possible to perform transport experiments in a single plate containing about one million cells. The cells were viable, intact and adhered to the supporting medium throughout the experiment. The AIB transport into both types of cells was Na+-dependent and showed saturation kinetics when the small component of the transport due to diffusion had been subtracted. The AIB transport capacity of neoplastic glioma cells was 3.6 times higher than that of glia cells. This difference was related to the Vmax-values for the two types of cells. The apparent Km-values were the same. Inhibition experiments with other amino acids support the view that AIB is transported via System A in both glia and glioma cells. Sulfhydryl reagents (ethacrynic acid and NEM) and cytochalasin B clearly inhibited the AIB transport into glia cells whereas the effect on glioma cells was minimal.", "contents": "Alpha-aminoisobutyric acid transport into human glia and glioma cells in culture. The AIB transport into human glia and glioma cells in culture has been studied. Because of the high affinity of AIB to the plastic culture dishes, a special washing technique had to be developed. With this technique, it was possible to perform transport experiments in a single plate containing about one million cells. The cells were viable, intact and adhered to the supporting medium throughout the experiment. The AIB transport into both types of cells was Na+-dependent and showed saturation kinetics when the small component of the transport due to diffusion had been subtracted. The AIB transport capacity of neoplastic glioma cells was 3.6 times higher than that of glia cells. This difference was related to the Vmax-values for the two types of cells. The apparent Km-values were the same. Inhibition experiments with other amino acids support the view that AIB is transported via System A in both glia and glioma cells. Sulfhydryl reagents (ethacrynic acid and NEM) and cytochalasin B clearly inhibited the AIB transport into glia cells whereas the effect on glioma cells was minimal.", "PMID": 977662}
{"id": "pubmed23n0032_11243", "title": "Different recovery patterns of mouse haemopoietic stem cells in response to cytotoxic agents.", "content": "The response and subsequent recovery of mouse haemopoietic progenitor cells (spleen colony forming cells and agar colony forming cells) has been studied following two cytotoxic agents. Busulphan was administered to normal mice and vinblastine to mice where the progenitor cell proliferation rate had been increased by a period of continuous gamma-irradiation. With both these agents there is a difference between the response of the spleen colony forming cells and the agar colony forming cells during the first five days. They then recover together, but much more slowly after fusulphan than after vinblastine even though their proliferation rate is increased. The rate of progenitor cell recovery after busulphan is increased if the progenitor cells are depleted further by vinblastine. However, methotrexate, which severely depletes the peripheral blood count and bone marrow cellularity but not the progenitor cells, has no effect on the recovery following busulphan. These results suggest that following cytotoxic agents the agar colony forming cells (\"committed\" stem cells) are not self-maintaining but are dependent on a supply of cells from the pluripotential spleen colony forming cells. In addition it appears that the depletion of the progenitor cells of the bone marrow and not the depletion of the maturing cells, provides a stimulus for stem cell recovery.", "contents": "Different recovery patterns of mouse haemopoietic stem cells in response to cytotoxic agents. The response and subsequent recovery of mouse haemopoietic progenitor cells (spleen colony forming cells and agar colony forming cells) has been studied following two cytotoxic agents. Busulphan was administered to normal mice and vinblastine to mice where the progenitor cell proliferation rate had been increased by a period of continuous gamma-irradiation. With both these agents there is a difference between the response of the spleen colony forming cells and the agar colony forming cells during the first five days. They then recover together, but much more slowly after fusulphan than after vinblastine even though their proliferation rate is increased. The rate of progenitor cell recovery after busulphan is increased if the progenitor cells are depleted further by vinblastine. However, methotrexate, which severely depletes the peripheral blood count and bone marrow cellularity but not the progenitor cells, has no effect on the recovery following busulphan. These results suggest that following cytotoxic agents the agar colony forming cells (\"committed\" stem cells) are not self-maintaining but are dependent on a supply of cells from the pluripotential spleen colony forming cells. In addition it appears that the depletion of the progenitor cells of the bone marrow and not the depletion of the maturing cells, provides a stimulus for stem cell recovery.", "PMID": 977663}
{"id": "pubmed23n0032_11244", "title": "A possible involvement polyamines in the initiation of DNA synthesis by human WI-38 and mouse BALB/3T3 cells.", "content": "Changing the medium, or adding fresh serum, induces a large proportion of the proliferatively quiescent cells in confluent monolayers of human WI-38 and mouse BALB/3T3 cells to initiate a growth-division cycle. Exposure at the time of the medium change or serum addition to MGBG (methyl glyoxal bis [guanylhydrazone]), an inhibitor of spermidine and spermine synthesis and function, reduces or stops the subsequent flow of cells into the DNA-synthetic phase, without grossly affecting RNA synthesis. Mediation of MGBG action by an actual or functional shortage of spermidine or spermine (but not putrescine), and consequently an involvement of these polyamines in DNA synthesis, is strongly suggested by the reduction of the inhibitor's effectiveness by a brief (1-hour), early prereplicative exposure of the treated cells to exogenous spermidine and spermine (but not putrescine).", "contents": "A possible involvement polyamines in the initiation of DNA synthesis by human WI-38 and mouse BALB/3T3 cells. Changing the medium, or adding fresh serum, induces a large proportion of the proliferatively quiescent cells in confluent monolayers of human WI-38 and mouse BALB/3T3 cells to initiate a growth-division cycle. Exposure at the time of the medium change or serum addition to MGBG (methyl glyoxal bis [guanylhydrazone]), an inhibitor of spermidine and spermine synthesis and function, reduces or stops the subsequent flow of cells into the DNA-synthetic phase, without grossly affecting RNA synthesis. Mediation of MGBG action by an actual or functional shortage of spermidine or spermine (but not putrescine), and consequently an involvement of these polyamines in DNA synthesis, is strongly suggested by the reduction of the inhibitor's effectiveness by a brief (1-hour), early prereplicative exposure of the treated cells to exogenous spermidine and spermine (but not putrescine).", "PMID": 977664}
{"id": "pubmed23n0032_11245", "title": "Human erythroid colony formation in vitro: evidence for clonal origin.", "content": "Human marrow cells, suspended in methylcellulose medium containing erythropoietin, give rise to discrete colonies of hemoglobin synthesizing cells. The presumption that such colonies originate from single progenitor cells has been tested directly in females with X-chromosome inactivation mosaicism using glucose-6-phosphate dehydrogenase (G-6-PD) as a marker. When individual colonies were grown from marrow cells obtained from two black females heterozygous for G-6-PD, only one or the other isoenzyme type was observed, but not both. These results are most consistent with the interpretation that human erythroid colonies arise from single cells.", "contents": "Human erythroid colony formation in vitro: evidence for clonal origin. Human marrow cells, suspended in methylcellulose medium containing erythropoietin, give rise to discrete colonies of hemoglobin synthesizing cells. The presumption that such colonies originate from single progenitor cells has been tested directly in females with X-chromosome inactivation mosaicism using glucose-6-phosphate dehydrogenase (G-6-PD) as a marker. When individual colonies were grown from marrow cells obtained from two black females heterozygous for G-6-PD, only one or the other isoenzyme type was observed, but not both. These results are most consistent with the interpretation that human erythroid colonies arise from single cells.", "PMID": 977665}
{"id": "pubmed23n0032_11246", "title": "The ordered columnar structure of mouse filiform papillae.", "content": "Examination of carefully oriented 1-mum and 5-mum sections of mouse dorsal tongue together with scanning electron microscope observations indicates a high degree of cellular organization in the papillae. It has been suggested that each filiform papilla consists of 2 dominant and 2 minor columns of cells. Labelling patterns of the basal cells have been investigated in relation to these columns.", "contents": "The ordered columnar structure of mouse filiform papillae. Examination of carefully oriented 1-mum and 5-mum sections of mouse dorsal tongue together with scanning electron microscope observations indicates a high degree of cellular organization in the papillae. It has been suggested that each filiform papilla consists of 2 dominant and 2 minor columns of cells. Labelling patterns of the basal cells have been investigated in relation to these columns.", "PMID": 977666}
{"id": "pubmed23n0032_11247", "title": "Sulphure in epidermal keratohyalin granules: a quantitative assay by x-ray microanalysis.", "content": "The elemental composition of different types of keratohyalin granules from the epidermis of newborn and adult rats was studied by means of an EMMA-4 analytical electron microscope, equipped with an energy-dispersive X-ray spectrometer. An absolute quantitation of the sulphur concentration in keratohyalin granules was performed. The results demonstrate that epidermal keratohyalin granules are chemically heterogeneous. A type of keratohyalin granule present in the nuclei and cytoplasm of epidermal cells from both newborn and adult rats - termed single granules - is rich in sulphur, having a content of 2-5-3-6%. Other types of keratohyalin granules, which differ in newborn and adult rats, contain a sulphur-poor component; they often have a sulphur-rich component as well. The sulphur-poor keratohyalin contains 0-6-0-9% sulphur. It is suggested that the sulphur-rich keratohyalin granules are the source of the peripheral envelope protein of cornified cells.", "contents": "Sulphure in epidermal keratohyalin granules: a quantitative assay by x-ray microanalysis. The elemental composition of different types of keratohyalin granules from the epidermis of newborn and adult rats was studied by means of an EMMA-4 analytical electron microscope, equipped with an energy-dispersive X-ray spectrometer. An absolute quantitation of the sulphur concentration in keratohyalin granules was performed. The results demonstrate that epidermal keratohyalin granules are chemically heterogeneous. A type of keratohyalin granule present in the nuclei and cytoplasm of epidermal cells from both newborn and adult rats - termed single granules - is rich in sulphur, having a content of 2-5-3-6%. Other types of keratohyalin granules, which differ in newborn and adult rats, contain a sulphur-poor component; they often have a sulphur-rich component as well. The sulphur-poor keratohyalin contains 0-6-0-9% sulphur. It is suggested that the sulphur-rich keratohyalin granules are the source of the peripheral envelope protein of cornified cells.", "PMID": 977667}
{"id": "pubmed23n0032_11248", "title": "Rapid modification of the morphology of cell contact sites during the aggregation of limpet haemocytes.", "content": "Haemocytes suspended in the blood of the limpet Patella vulgata rapidly form microspikes and aggregate in response to withdrawal from the haemocoel. It has previously been suggested that the contacts between colliding cells in shaken suspension are stabilized by the rapid spreading of participant cells over each other's surfaces by a microspike-dependent process. In this communication we report that an electron-microscopic study of the contacts formed between cells in the early stages of aggregation suggests that intercellular contact elicits a rapid response from the organelles concerned with cell motility. This response leads to the rapid progression of each initial collision through 3 major categories of contact morphology, namely cell body/lamella, lamella/lamella and cell body/cell body. There is a concomitant increase in the area of mutually contacting surface between participating cells. On the basis of these results, a model is constructed for the participation of sub-surface components in the process of cellular aggregation. The sequence of development of contact found between aggregating cells is envisaged to be a consequence of cellular locomotory activities resembling those by which these cells spread on to a solid substrate. Included in this process is an element of intercellular recognition. It is suggested that the involvement of motile elements in cell aggregation is a general feature of aggregating cells. This would permit a re-interpretation of aggregation experiments which show specificity of cell adhesion, for total adhesive interactions between cells would depend not only on the adhesiveness per unit area of contacting cell surface but also on the extent or rate of formation of flat contact areas. The latter element could be regulated by a recognition system between contacting cells independently of the force per unit area which holds the 2 surfaces together.", "contents": "Rapid modification of the morphology of cell contact sites during the aggregation of limpet haemocytes. Haemocytes suspended in the blood of the limpet Patella vulgata rapidly form microspikes and aggregate in response to withdrawal from the haemocoel. It has previously been suggested that the contacts between colliding cells in shaken suspension are stabilized by the rapid spreading of participant cells over each other's surfaces by a microspike-dependent process. In this communication we report that an electron-microscopic study of the contacts formed between cells in the early stages of aggregation suggests that intercellular contact elicits a rapid response from the organelles concerned with cell motility. This response leads to the rapid progression of each initial collision through 3 major categories of contact morphology, namely cell body/lamella, lamella/lamella and cell body/cell body. There is a concomitant increase in the area of mutually contacting surface between participating cells. On the basis of these results, a model is constructed for the participation of sub-surface components in the process of cellular aggregation. The sequence of development of contact found between aggregating cells is envisaged to be a consequence of cellular locomotory activities resembling those by which these cells spread on to a solid substrate. Included in this process is an element of intercellular recognition. It is suggested that the involvement of motile elements in cell aggregation is a general feature of aggregating cells. This would permit a re-interpretation of aggregation experiments which show specificity of cell adhesion, for total adhesive interactions between cells would depend not only on the adhesiveness per unit area of contacting cell surface but also on the extent or rate of formation of flat contact areas. The latter element could be regulated by a recognition system between contacting cells independently of the force per unit area which holds the 2 surfaces together.", "PMID": 977668}
{"id": "pubmed23n0032_11249", "title": "Some ultrastructural aspects of mitosis and early differentiation in human foetal lung.", "content": "In rapidly dividing human foetal lung cells there is evidence of synthesis of extra membrane prior to mitosis. During prophase the nuclear envelope breaks down and is conserved as long double or complex stacks during the following stages of mitosis. It is suggested that the membrane is retained partly for incorporation into the daughter cell nuclear envelopes and partly for the formation of RER. The relevance of the observations to the biochemical data in the literature concerning the formation of intracellular membranes is discussed briefly.", "contents": "Some ultrastructural aspects of mitosis and early differentiation in human foetal lung. In rapidly dividing human foetal lung cells there is evidence of synthesis of extra membrane prior to mitosis. During prophase the nuclear envelope breaks down and is conserved as long double or complex stacks during the following stages of mitosis. It is suggested that the membrane is retained partly for incorporation into the daughter cell nuclear envelopes and partly for the formation of RER. The relevance of the observations to the biochemical data in the literature concerning the formation of intracellular membranes is discussed briefly.", "PMID": 977669}
{"id": "pubmed23n0032_11250", "title": "The nucleolus organizer and the synaptonemal complex in Endymion non-scriptus (L.).", "content": "Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae of nucleoli. Its chromosomal nature was strongly suggested by the presence of the synaptonemal complex within it. This demonstrated that the pale-staining region of nucleoli is the nucleolus organizer and almost certainly the chromosome region containing the ribosomal cistrons, and justifies the use of these terms to describe the structure when seen inside the nucleolus. The relationship between this zone and the heterochromatic knob called the nucleolar organizing body in maize by other workers is discussed.", "contents": "The nucleolus organizer and the synaptonemal complex in Endymion non-scriptus (L.). Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae of nucleoli. Its chromosomal nature was strongly suggested by the presence of the synaptonemal complex within it. This demonstrated that the pale-staining region of nucleoli is the nucleolus organizer and almost certainly the chromosome region containing the ribosomal cistrons, and justifies the use of these terms to describe the structure when seen inside the nucleolus. The relationship between this zone and the heterochromatic knob called the nucleolar organizing body in maize by other workers is discussed.", "PMID": 977670}
{"id": "pubmed23n0032_11251", "title": "[Compression of retropancreatic common bile duct by tuberculous lymph nodes causing obstructive icterus].", "content": "Tuberculosis of the hepatic lymph nodes is among the rare causes of obstructive jaundice and portal hypertension. The authors report a case of compression of the bile duct by retroduodeno-pancreatic tuberculous lymphadenopathy, and recall the difficulty of diagnosis before operation. They emphasize the necessity of simple surgical measures. Removal of the lymph nodes is dangerous and should not be attempted. A biliary by-pass operation should be carried out and its type depends on the precise site of the compression. Antituberculous drugs are then given for 18 months after operation and lead to cure.", "contents": "[Compression of retropancreatic common bile duct by tuberculous lymph nodes causing obstructive icterus]. Tuberculosis of the hepatic lymph nodes is among the rare causes of obstructive jaundice and portal hypertension. The authors report a case of compression of the bile duct by retroduodeno-pancreatic tuberculous lymphadenopathy, and recall the difficulty of diagnosis before operation. They emphasize the necessity of simple surgical measures. Removal of the lymph nodes is dangerous and should not be attempted. A biliary by-pass operation should be carried out and its type depends on the precise site of the compression. Antituberculous drugs are then given for 18 months after operation and lead to cure.", "PMID": 977671}
{"id": "pubmed23n0032_11252", "title": "[Traumatic rupture of the cervical trachea. Emergency diagnosis and treatment].", "content": "The authors report 3 cases of traumatic rupture of the cervical trachea including one associated with total division of the oesophagus. They were impressed by the lack of precision and rapidity of the first surgical measures. They then give an account of the therapeutic gestures which are possible in a non-specialised unit.", "contents": "[Traumatic rupture of the cervical trachea. Emergency diagnosis and treatment]. The authors report 3 cases of traumatic rupture of the cervical trachea including one associated with total division of the oesophagus. They were impressed by the lack of precision and rapidity of the first surgical measures. They then give an account of the therapeutic gestures which are possible in a non-specialised unit.", "PMID": 977672}
{"id": "pubmed23n0032_11253", "title": "[Protrusion of the femoral vessels into the obturator foramen associated with fracture of the pelvis. Surgical treatment].", "content": "The authors were led to operate for a severe ischemic syndrome complicating fracture of the pelvis, a patient who presented with curious mobilisation of the femoral vessels in the obturator foramen with arterial and venous compression and who benefitted from treatment by simple reduction.", "contents": "[Protrusion of the femoral vessels into the obturator foramen associated with fracture of the pelvis. Surgical treatment]. The authors were led to operate for a severe ischemic syndrome complicating fracture of the pelvis, a patient who presented with curious mobilisation of the femoral vessels in the obturator foramen with arterial and venous compression and who benefitted from treatment by simple reduction.", "PMID": 977673}
{"id": "pubmed23n0032_11254", "title": "[Supra-condylar fractures in the child].", "content": "The author recalls the technical rules for reduction of supra-condylar fractures in children by the method of percutaneous nailing. Complications and failures of this method are rare and usually due to mistakes. The treatement of sequelae is then considered briefly.", "contents": "[Supra-condylar fractures in the child]. The author recalls the technical rules for reduction of supra-condylar fractures in children by the method of percutaneous nailing. Complications and failures of this method are rare and usually due to mistakes. The treatement of sequelae is then considered briefly.", "PMID": 977674}
{"id": "pubmed23n0032_11255", "title": "[Aneurysms, late complications at the level of anastomosis of an arterial bypass].", "content": "The authors report 12 cases of aneurysm occuring in anastomoses in 9 operated patients. The delay of onset of these lesions was long, 9 years on average. The fraquency appears low, slightly greater than 1 p. 100 in all arterial plastic operations. Among the 10 treated lesions half were false aneurysms due to breakdown of the suture and half were true aneurysms due to progression of the arterial dystrophy. All cases were treated by dissection and insertion of a new prosthesis between the old prosthesis and a healthy area, beyond the anastomosis. Out of 9 patients, there were 8 good immediate results and one failure due to infection at the level of the prosthesis. Among the various methods of prevention, avoiding infection may be the most important.", "contents": "[Aneurysms, late complications at the level of anastomosis of an arterial bypass]. The authors report 12 cases of aneurysm occuring in anastomoses in 9 operated patients. The delay of onset of these lesions was long, 9 years on average. The fraquency appears low, slightly greater than 1 p. 100 in all arterial plastic operations. Among the 10 treated lesions half were false aneurysms due to breakdown of the suture and half were true aneurysms due to progression of the arterial dystrophy. All cases were treated by dissection and insertion of a new prosthesis between the old prosthesis and a healthy area, beyond the anastomosis. Out of 9 patients, there were 8 good immediate results and one failure due to infection at the level of the prosthesis. Among the various methods of prevention, avoiding infection may be the most important.", "PMID": 977675}
{"id": "pubmed23n0032_11256", "title": "[Critical study of electric activity of the small intestine in the dog].", "content": "Thanks to a cross-circulation, the authors caused to survive in the abdomen of a sacrificed dog, the whole of the small intestine, the motor activity of which was analysed by electro-enterography. This experimental preparation thus eliminates polygraphic recordings of all potential variations outside the small intestine, e.g. stomach, colon, diaphragm, muscle, myocardium. One thus obtains very pure tracings of the variations of potential originating in the small intestine, recorded from bipolar serous intestinal electrodes, they show the activity of the small intestine, consisting of a permanent basal electric rhythm on which are grafted spikes contemporary of motor phenomena. In the abdominal skin there were recorded only a cutaneous slow wave which was contemporary with the deep motor phenomena.", "contents": "[Critical study of electric activity of the small intestine in the dog]. Thanks to a cross-circulation, the authors caused to survive in the abdomen of a sacrificed dog, the whole of the small intestine, the motor activity of which was analysed by electro-enterography. This experimental preparation thus eliminates polygraphic recordings of all potential variations outside the small intestine, e.g. stomach, colon, diaphragm, muscle, myocardium. One thus obtains very pure tracings of the variations of potential originating in the small intestine, recorded from bipolar serous intestinal electrodes, they show the activity of the small intestine, consisting of a permanent basal electric rhythm on which are grafted spikes contemporary of motor phenomena. In the abdominal skin there were recorded only a cutaneous slow wave which was contemporary with the deep motor phenomena.", "PMID": 977676}
{"id": "pubmed23n0032_11257", "title": "[Efficiency of revascularization of the liver by intra-hepatic implantation of the splenic vascular pedicle].", "content": "Experiments carried out in 12 dogs with ligature of the hepatic lobar artery, show that intra-hepatic implantation of the splenic artery, or vascular pedicle, offers limited revascularisation. This produces a fall in blood flow in the implanted artery, reduction in glycogen and hepatic A.T.P., associated with a fall in the total protein content and hepatosine. At the same time, although there is a rich network of neoformed vessels, the hepatic transaminase and lactate activities undergo a pronounced rise.", "contents": "[Efficiency of revascularization of the liver by intra-hepatic implantation of the splenic vascular pedicle]. Experiments carried out in 12 dogs with ligature of the hepatic lobar artery, show that intra-hepatic implantation of the splenic artery, or vascular pedicle, offers limited revascularisation. This produces a fall in blood flow in the implanted artery, reduction in glycogen and hepatic A.T.P., associated with a fall in the total protein content and hepatosine. At the same time, although there is a rich network of neoformed vessels, the hepatic transaminase and lactate activities undergo a pronounced rise.", "PMID": 977677}
{"id": "pubmed23n0032_11258", "title": "High-performance liquid chromatography of proteins.", "content": "Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase in 6 min.", "contents": "High-performance liquid chromatography of proteins. Proteins were separated on microparticulate bonded phase steric exclusion and anion-exchange chromatography supports. A post-column enzyme detector was developed which gives a specific and sensitive response for enzymes. The three iso-enzymes of creatine phosphokinase were separated and assayed in 4 min and the five isoenzymes of lactic dehydrogenase in 6 min.", "PMID": 977678}
{"id": "pubmed23n0032_11259", "title": "A low-cost gradient system for high-performance liquid chromatography. Quantitation of complex pharmaceutical raw materials.", "content": "A device is described that makes use of an eight-port motor valve to generate step gradients on the low-pressure side of a piston pump with a low dead volume. Such a gradient device with an automatic control unit, which also permits repetition of previous steps, can be built for about half the cost of a gradient system with two pumps. Applications of this gradient unit to the separation of complex mixtures of glycosides and alkaloids are discussed and compared with separations systems using two high-pressure pumps. The gradients that are used on reversed-phase material with solvent mixtures of water and completely miscible organic solvents are suitable for quantitative routine control of pharmaceutical products. The reproducibility of retention data is excellent over several months and, with the use of loop injectors, major components can be determined quantitatively with a reproducibility of better than 2% (relative standard deviation). The step gradient selector valve can also be used as an introduction system for very large sample volumes. Up to 11 can be injected and samples with concentrations of less than 1 ppb can be determined with good reproducibilities.", "contents": "A low-cost gradient system for high-performance liquid chromatography. Quantitation of complex pharmaceutical raw materials. A device is described that makes use of an eight-port motor valve to generate step gradients on the low-pressure side of a piston pump with a low dead volume. Such a gradient device with an automatic control unit, which also permits repetition of previous steps, can be built for about half the cost of a gradient system with two pumps. Applications of this gradient unit to the separation of complex mixtures of glycosides and alkaloids are discussed and compared with separations systems using two high-pressure pumps. The gradients that are used on reversed-phase material with solvent mixtures of water and completely miscible organic solvents are suitable for quantitative routine control of pharmaceutical products. The reproducibility of retention data is excellent over several months and, with the use of loop injectors, major components can be determined quantitatively with a reproducibility of better than 2% (relative standard deviation). The step gradient selector valve can also be used as an introduction system for very large sample volumes. Up to 11 can be injected and samples with concentrations of less than 1 ppb can be determined with good reproducibilities.", "PMID": 977679}
{"id": "pubmed23n0032_11260", "title": "Anodic coulometric detection with a glassy carbon electrode in combination with reversed-phase high-performance liquid chromatography. Determination of blood levels of perphenazine and fluphenazine.", "content": "A detector for liquid chromatography, based on anodic electrochemical oxidation at a glassy carbon electrode, is used in combination with reversed-phase liquid chromatography for the determination of the long-acting tricyclic psychotropic drugs fluphenazine and perphenazine in blood. Completely porous microparticulate silica material, modified so as to give a methyl silica, was used as the stationary phase and buffered mixtures of methanol and water as the mobile phase. The low detection limit and the selectivity of the coulometric detector made possible the determination of concentrations of 1-20 ppb of the compounds of interest. An extraction procedure was in order to obtain sufficient selectivity. Data for the precision, detection limit and yield of the extraction procedure are given. A number of serum levels of perphenazine after oral administration and of fluphenazine after i.m. doses of fluphenazine decanoate are stated.", "contents": "Anodic coulometric detection with a glassy carbon electrode in combination with reversed-phase high-performance liquid chromatography. Determination of blood levels of perphenazine and fluphenazine. A detector for liquid chromatography, based on anodic electrochemical oxidation at a glassy carbon electrode, is used in combination with reversed-phase liquid chromatography for the determination of the long-acting tricyclic psychotropic drugs fluphenazine and perphenazine in blood. Completely porous microparticulate silica material, modified so as to give a methyl silica, was used as the stationary phase and buffered mixtures of methanol and water as the mobile phase. The low detection limit and the selectivity of the coulometric detector made possible the determination of concentrations of 1-20 ppb of the compounds of interest. An extraction procedure was in order to obtain sufficient selectivity. Data for the precision, detection limit and yield of the extraction procedure are given. A number of serum levels of perphenazine after oral administration and of fluphenazine after i.m. doses of fluphenazine decanoate are stated.", "PMID": 977680}
{"id": "pubmed23n0032_11261", "title": "High-performance liquid chromatographic analysis of porphyrins in clinical materials.", "content": "Methods for the isolation of porphyrins as their methyl esters from porphyric urine and faeces as well as other biological materials are described. Quantitative analyses can be carried out by high-performance liquid chromatography (HPLC), using appropriate internal standards; hence excretion patterns in the various types of porphyria can be obtained which may facilitate clinical diagnosis more effectively than the earlier qualitative thin-layer chromatographic methods. Use of the newer microparticulate column packing materials has improved the efficiency of the HPLC analyses, and enables the more convenient isochratic elution techniques to be used (rather than gradient elution). Separations of some porphyrin isomers on these columns are also described.", "contents": "High-performance liquid chromatographic analysis of porphyrins in clinical materials. Methods for the isolation of porphyrins as their methyl esters from porphyric urine and faeces as well as other biological materials are described. Quantitative analyses can be carried out by high-performance liquid chromatography (HPLC), using appropriate internal standards; hence excretion patterns in the various types of porphyria can be obtained which may facilitate clinical diagnosis more effectively than the earlier qualitative thin-layer chromatographic methods. Use of the newer microparticulate column packing materials has improved the efficiency of the HPLC analyses, and enables the more convenient isochratic elution techniques to be used (rather than gradient elution). Separations of some porphyrin isomers on these columns are also described.", "PMID": 977681}
{"id": "pubmed23n0032_11262", "title": "High-pressure liquid chromatography of steroids secreted by human adrenal and testis cells in monolayer culture.", "content": "Reversed-phase high-pressure liquid chromatography with gradient elution on Zorbax-ODS columns has been used to separate, identify, and measure, spectrophotometrically, the steroids secreted by both human adrenal and testis cells in primary monolayer culture. Three related systems using exponential concave gradients have been developed with the specific objective of resolving the steroids produced by these two tissues. A methanol-water gradient has been used to separate most adrenal steroids, an acetonitrile-water gradient to separate testis steroids, and a dioxane-water gradient to separate polar steroids, including aldosterone. These three systems together permit the resolution of at least 43 naturally occurring steroids, plus four synthetic steroids with adrenocortical activity, with overall total elution times of 1 h or less for each system. Retention data for these steroids are given and the separation of steroids in the biological samples illustrated.", "contents": "High-pressure liquid chromatography of steroids secreted by human adrenal and testis cells in monolayer culture. Reversed-phase high-pressure liquid chromatography with gradient elution on Zorbax-ODS columns has been used to separate, identify, and measure, spectrophotometrically, the steroids secreted by both human adrenal and testis cells in primary monolayer culture. Three related systems using exponential concave gradients have been developed with the specific objective of resolving the steroids produced by these two tissues. A methanol-water gradient has been used to separate most adrenal steroids, an acetonitrile-water gradient to separate testis steroids, and a dioxane-water gradient to separate polar steroids, including aldosterone. These three systems together permit the resolution of at least 43 naturally occurring steroids, plus four synthetic steroids with adrenocortical activity, with overall total elution times of 1 h or less for each system. Retention data for these steroids are given and the separation of steroids in the biological samples illustrated.", "PMID": 977682}
{"id": "pubmed23n0032_11263", "title": "Quantitative determination of low levels of daunomycin and daunomycinol in plasma by high-performance liquid chromatography.", "content": "A high-performance liquid chromatographic method for the measurement of daunomycin and its main metabolite, daunomycinol, at low concentrations in plasma is described. Quantitative determinations were obtained by the use of adriamycin, another cystostatic anthracycline antibiotic, as an internal standard. The separations were carried out on a 5-mum silica microsphere column with a quaternary solvent mixture as eluent. The components in the eluted peaks were detected by visible absorption at 490 nm and there were no interfering peaks. The advantages of the method are specificity, sensitivity, minimal pre-analysis sample work-up and small sample size. The method is sensitive for plasma levels of daunomycin and daunomycinol above 10 ng/ml. Experimental animal data are presented to illustrate the application of the method.", "contents": "Quantitative determination of low levels of daunomycin and daunomycinol in plasma by high-performance liquid chromatography. A high-performance liquid chromatographic method for the measurement of daunomycin and its main metabolite, daunomycinol, at low concentrations in plasma is described. Quantitative determinations were obtained by the use of adriamycin, another cystostatic anthracycline antibiotic, as an internal standard. The separations were carried out on a 5-mum silica microsphere column with a quaternary solvent mixture as eluent. The components in the eluted peaks were detected by visible absorption at 490 nm and there were no interfering peaks. The advantages of the method are specificity, sensitivity, minimal pre-analysis sample work-up and small sample size. The method is sensitive for plasma levels of daunomycin and daunomycinol above 10 ng/ml. Experimental animal data are presented to illustrate the application of the method.", "PMID": 977683}
{"id": "pubmed23n0032_11264", "title": "Separation of catecholamines and their metabolites by adsorption, ion-pair and soap chromatography.", "content": "The separation of catecholamines and their metabolites has been carried out by high-performance liquid chromatography using three systems: liquid-solid adsorption, ion-pair partition, and soap chromatography. In the last newly developed technique a reversed-phase packing is used in conjunction with an aqueous organic eluent containing a detergent. The detergent is chosen so that its ion can form ion-pairs with ions of the solutes. Soap chromatography proved the best technique in terms of column efficiency (giving 3000-5000 plates in 125 mm), resolution and sensitivity of detection. Noradrenaline, adrenaline and dopamine, their 3-O-methyl derivatives, L-3,4-dihydroxyphenylalanine, homovanillic acid and other related compounds could be separated in less than 10 min at the 10-50-ng level on columns whose plate heights were in the range of 20-40 mum. The dependence of retention on the concentration of organic modifier and on detergent concentration for three anionic detergents is reported. The method is applied to the direct analysis of urine and the potential of the method for such analyses, especially of pathological urines is demonstrated. Soap chromatography is likely to enlarge the scope of application of high-performance liquid chromatography to biochemical analysis. It is a powerful method for the separation of ionizable compounds which could replace conventional ion-exchange chromatography.", "contents": "Separation of catecholamines and their metabolites by adsorption, ion-pair and soap chromatography. The separation of catecholamines and their metabolites has been carried out by high-performance liquid chromatography using three systems: liquid-solid adsorption, ion-pair partition, and soap chromatography. In the last newly developed technique a reversed-phase packing is used in conjunction with an aqueous organic eluent containing a detergent. The detergent is chosen so that its ion can form ion-pairs with ions of the solutes. Soap chromatography proved the best technique in terms of column efficiency (giving 3000-5000 plates in 125 mm), resolution and sensitivity of detection. Noradrenaline, adrenaline and dopamine, their 3-O-methyl derivatives, L-3,4-dihydroxyphenylalanine, homovanillic acid and other related compounds could be separated in less than 10 min at the 10-50-ng level on columns whose plate heights were in the range of 20-40 mum. The dependence of retention on the concentration of organic modifier and on detergent concentration for three anionic detergents is reported. The method is applied to the direct analysis of urine and the potential of the method for such analyses, especially of pathological urines is demonstrated. Soap chromatography is likely to enlarge the scope of application of high-performance liquid chromatography to biochemical analysis. It is a powerful method for the separation of ionizable compounds which could replace conventional ion-exchange chromatography.", "PMID": 977684}
{"id": "pubmed23n0032_11265", "title": "Determination of naloxone and naltrexone as perfluoroalkyl ester derivatives by electron-capture gas-liquid chromatography.", "content": "An electron-capture gas chromatographic method is described for the determination of naloxone and naltrexone as the perfluoroalkyl esters. Each compound serves as internal standard for determination of the other. The method permits quantitation of 2-100 ng of either compound. Conditions for derivatization with heptafluorobutyric anhydride (HFBA), pentafluoropropionic anhydride (PFPA), and trifluoroacetic anhydride (TFAA) have been investigated. When catalyzed with pyridine, derivatization with HFBA and PFPA at 70 degrees gives naloxone and naltrexone triesters. Evidence for triester formation was obtained from gas chromatography-methane chemical ionization mass spectrometry and infrared spectral analysis. It was found that both the HFB and PFP triesters are suitable for quantitation of the narcotic antagonists, the HFB derivatives having greater stability than the PFP derivatives. The TFA derivatives are substantially less stable.", "contents": "Determination of naloxone and naltrexone as perfluoroalkyl ester derivatives by electron-capture gas-liquid chromatography. An electron-capture gas chromatographic method is described for the determination of naloxone and naltrexone as the perfluoroalkyl esters. Each compound serves as internal standard for determination of the other. The method permits quantitation of 2-100 ng of either compound. Conditions for derivatization with heptafluorobutyric anhydride (HFBA), pentafluoropropionic anhydride (PFPA), and trifluoroacetic anhydride (TFAA) have been investigated. When catalyzed with pyridine, derivatization with HFBA and PFPA at 70 degrees gives naloxone and naltrexone triesters. Evidence for triester formation was obtained from gas chromatography-methane chemical ionization mass spectrometry and infrared spectral analysis. It was found that both the HFB and PFP triesters are suitable for quantitation of the narcotic antagonists, the HFB derivatives having greater stability than the PFP derivatives. The TFA derivatives are substantially less stable.", "PMID": 977685}
{"id": "pubmed23n0032_11266", "title": "Quantitative determination of hexosamines in glycoprotein by ion-exchange chromatography.", "content": "An ion-exchange chromatographic method for the quantitative determination of glucosamine and galactosamine in glycoproteins is described. The hexosamines are completely separated from interfering peptides of the acid hydrolysate using a small column of cation exchanger in a manner described earlier by Boas. Chromatographic separation of the hexosamine fraction and the determination of glucosamine and galactosamine are accomplished on an amino acid analyzer with ninhydrin.", "contents": "Quantitative determination of hexosamines in glycoprotein by ion-exchange chromatography. An ion-exchange chromatographic method for the quantitative determination of glucosamine and galactosamine in glycoproteins is described. The hexosamines are completely separated from interfering peptides of the acid hydrolysate using a small column of cation exchanger in a manner described earlier by Boas. Chromatographic separation of the hexosamine fraction and the determination of glucosamine and galactosamine are accomplished on an amino acid analyzer with ninhydrin.", "PMID": 977686}
{"id": "pubmed23n0032_11267", "title": "New method for the preparation of highly stable polysiloxane-coated glass open-tubular capillary columns and application to the analysis of hormonal steroids.", "content": "A new approach to the preparation of methylsiloxane-coated glass open-tubular capillary columns is described. A siloxane polymeric mixture is first prepared by hydrolysis of dimethyldichlorosilane. The polymer is then coated on to the previously etched glass wall by a base-catalyzed reaction to yield a non-polar system of high stability and showing satisfactory chromatographic properties. This type of column has been used for the high-resolution separations of complex hormonal steroid mixtures. The flexibility of this method should permit the preparation of capillary systems with a wide range of polarity.", "contents": "New method for the preparation of highly stable polysiloxane-coated glass open-tubular capillary columns and application to the analysis of hormonal steroids. A new approach to the preparation of methylsiloxane-coated glass open-tubular capillary columns is described. A siloxane polymeric mixture is first prepared by hydrolysis of dimethyldichlorosilane. The polymer is then coated on to the previously etched glass wall by a base-catalyzed reaction to yield a non-polar system of high stability and showing satisfactory chromatographic properties. This type of column has been used for the high-resolution separations of complex hormonal steroid mixtures. The flexibility of this method should permit the preparation of capillary systems with a wide range of polarity.", "PMID": 977689}
{"id": "pubmed23n0032_11268", "title": "Rapid quantitative microanalysis of ketones in urine by gas chromatography-mass fragmentography.", "content": "A quantitative method is described for determination of 4-heptanone and its precursor in urine. The method involves a single-step extraction of urine with cyclohexane and the analysis of 1 mul of the extract by gas chromatography-mass fragmentography. The detection limit is 8-10(-12)g, corresponding to 2 mug per 1000 ml of urine. The method is linear in the concentration range of 7.6-10(-9)-1.5-10(-5)g/ml and the relative standard deviations for the individual values are between 1.2 and 10%. Approximately 40 samples can be analyzed per day or 1 sample in 7-8 min. By varying the experimental conditions, the method is applicable to other ketones.", "contents": "Rapid quantitative microanalysis of ketones in urine by gas chromatography-mass fragmentography. A quantitative method is described for determination of 4-heptanone and its precursor in urine. The method involves a single-step extraction of urine with cyclohexane and the analysis of 1 mul of the extract by gas chromatography-mass fragmentography. The detection limit is 8-10(-12)g, corresponding to 2 mug per 1000 ml of urine. The method is linear in the concentration range of 7.6-10(-9)-1.5-10(-5)g/ml and the relative standard deviations for the individual values are between 1.2 and 10%. Approximately 40 samples can be analyzed per day or 1 sample in 7-8 min. By varying the experimental conditions, the method is applicable to other ketones.", "PMID": 977690}
{"id": "pubmed23n0032_11269", "title": "Column elution and concentration of volatile compounds in biological fluids.", "content": "A new solvent elution method is described for the isolation and concentration of volatile metabolites in biological fluids. The procedure involves elution of the sample with a solvent through a micro-column of an adsorbent into glass-wool and evaporation of the excess solvent in a stream of helium at room temperature. The volatiles are revocered by subsequent heat desorption into a chromatogrpaphic system. Both elution and evaporation are made in a single step using a novel sampling device. Since the technique is based on liquid-solid adsorption chromatography, the adsorbent-solvent system which gives the maximum yield of volatile constituents was studied. The method is simple, highly efficient and reproducible, requiring only small volumes of biological fluids (less than 100 mul).", "contents": "Column elution and concentration of volatile compounds in biological fluids. A new solvent elution method is described for the isolation and concentration of volatile metabolites in biological fluids. The procedure involves elution of the sample with a solvent through a micro-column of an adsorbent into glass-wool and evaporation of the excess solvent in a stream of helium at room temperature. The volatiles are revocered by subsequent heat desorption into a chromatogrpaphic system. Both elution and evaporation are made in a single step using a novel sampling device. Since the technique is based on liquid-solid adsorption chromatography, the adsorbent-solvent system which gives the maximum yield of volatile constituents was studied. The method is simple, highly efficient and reproducible, requiring only small volumes of biological fluids (less than 100 mul).", "PMID": 977691}
{"id": "pubmed23n0032_11270", "title": "Profiles of volatile metabolities in body fluids.", "content": "A method for the analysis of volatile metabolites present in plasma, urine, breast milk and amniotic fluid collected from mother-infant pairs has been developed which requires only 100 mul of plasma, 3 ml of urine, 20 mul of breast milk and 500 mul of amniotic fluid. After extraction with diethyl ether, the volatile compounds were absorbed on glass wool in a special concentration tube and subsequently desorbed and transferred to a 100-m nickel capillary column for analysis by gas chromatography and gas chromatography-mass spectrometry. The separations, carried out by temperature programming, were complete in 90 min.", "contents": "Profiles of volatile metabolities in body fluids. A method for the analysis of volatile metabolites present in plasma, urine, breast milk and amniotic fluid collected from mother-infant pairs has been developed which requires only 100 mul of plasma, 3 ml of urine, 20 mul of breast milk and 500 mul of amniotic fluid. After extraction with diethyl ether, the volatile compounds were absorbed on glass wool in a special concentration tube and subsequently desorbed and transferred to a 100-m nickel capillary column for analysis by gas chromatography and gas chromatography-mass spectrometry. The separations, carried out by temperature programming, were complete in 90 min.", "PMID": 977692}
{"id": "pubmed23n0032_11271", "title": "Picogram analysis of free triiodothyronine and free thyroxine hormones in serum by equilibrium dialysis and electron capture gas chromatography.", "content": "A methodology involving equilibrium dialysis and gas chromatography has been developed to measure concurrently the concentration of the dialyzable (i.e., free) fractions of thyroxine and 3,5,3'-triiodothyronine in serum. The electron capture response of the N,0-diheptafluorobutyryl methyl ester derivatives of these hormones allows their detection and quantitation in the picogram range (detection limit approx. 0.2pg). Purification of the dialysate is achieved using a cation-exchange column which is pretreated with diiodothyronine. Adsorption problems on the glassware are eliminated by silanization. The methodology also resolves 3,3',5'-triiodothyronine (reverse T3).", "contents": "Picogram analysis of free triiodothyronine and free thyroxine hormones in serum by equilibrium dialysis and electron capture gas chromatography. A methodology involving equilibrium dialysis and gas chromatography has been developed to measure concurrently the concentration of the dialyzable (i.e., free) fractions of thyroxine and 3,5,3'-triiodothyronine in serum. The electron capture response of the N,0-diheptafluorobutyryl methyl ester derivatives of these hormones allows their detection and quantitation in the picogram range (detection limit approx. 0.2pg). Purification of the dialysate is achieved using a cation-exchange column which is pretreated with diiodothyronine. Adsorption problems on the glassware are eliminated by silanization. The methodology also resolves 3,3',5'-triiodothyronine (reverse T3).", "PMID": 977693}
{"id": "pubmed23n0032_11272", "title": "Determination of hydralazine and its metabolites by gas chromatography-mass spectrometry.", "content": "Metabolism of the vasodilator hydralazine was investigated by in vivo and in vitro studies. Standards to identify metabolic products were synthesized. Determination and quantification of hydralazine and its metabolites were accomplished by gas chromatography-mass spectrometry. A deuterium-labeled internal standard was used for quantification. 14C-labeled internal standards were synthesized and used to demonstrate recoveries from the biological samples.", "contents": "Determination of hydralazine and its metabolites by gas chromatography-mass spectrometry. Metabolism of the vasodilator hydralazine was investigated by in vivo and in vitro studies. Standards to identify metabolic products were synthesized. Determination and quantification of hydralazine and its metabolites were accomplished by gas chromatography-mass spectrometry. A deuterium-labeled internal standard was used for quantification. 14C-labeled internal standards were synthesized and used to demonstrate recoveries from the biological samples.", "PMID": 977694}
{"id": "pubmed23n0032_11273", "title": "Gas chromatographic study of free polyols and aldoses in cataractous human lens tissue.", "content": "An analytical procedure for the qualitative and quantitative analysis of human lens tissue for polyol and aldose content is described. Profile samples are obtained by direct derivatization of lyophilized lenses. The components are analyzed as per-O-acetylpolyols (from the polyols) and per-O-acetylaldononitriles (from the aldoses). This procedure converts each component into a single derivative and terminal dissymmetry for each aldose is retained. The derivatives form in quantitative yield, give good chromatographic peaks, are thermally stable and readily volatilized. They are not subject to adsorption on gas chromatographic columns and are suitable for both qualitative and quantitative analytical studies. Six non-cataractous lenses and fourteen lenses from patients with senile cataract (in seven instances complicated by diabetic pathology) were analyzed. Thermostable borosilicate glass open-tubular capillary columns, coated with the nonpolar phase SE-30, and containing dispersed particles of silanized silicic acid, were used for the gas chromatographic separations. The results are discussed in relation to what is known from earlier studies of human and animal cataracts. A gas chromatographic method for determining the polyol and aldose excretion levels of controlled diabetics is also reported along with a typical metabolic profile.", "contents": "Gas chromatographic study of free polyols and aldoses in cataractous human lens tissue. An analytical procedure for the qualitative and quantitative analysis of human lens tissue for polyol and aldose content is described. Profile samples are obtained by direct derivatization of lyophilized lenses. The components are analyzed as per-O-acetylpolyols (from the polyols) and per-O-acetylaldononitriles (from the aldoses). This procedure converts each component into a single derivative and terminal dissymmetry for each aldose is retained. The derivatives form in quantitative yield, give good chromatographic peaks, are thermally stable and readily volatilized. They are not subject to adsorption on gas chromatographic columns and are suitable for both qualitative and quantitative analytical studies. Six non-cataractous lenses and fourteen lenses from patients with senile cataract (in seven instances complicated by diabetic pathology) were analyzed. Thermostable borosilicate glass open-tubular capillary columns, coated with the nonpolar phase SE-30, and containing dispersed particles of silanized silicic acid, were used for the gas chromatographic separations. The results are discussed in relation to what is known from earlier studies of human and animal cataracts. A gas chromatographic method for determining the polyol and aldose excretion levels of controlled diabetics is also reported along with a typical metabolic profile.", "PMID": 977695}
{"id": "pubmed23n0032_11274", "title": "Study of the intestinal tyrosine metabolism using stable isotopes and gas chromatography-mass spectrometry.", "content": "Deuterated tyrosine, 4-hydroxyphenyllactic acid, 4-hydroxyphenylpropionic acid, 4-hydroxyphenylacetic acid and 4-hydroxybenzoic acid were incubated under anaerobic conditions with human faecal specimens for the in vitro study of their respective metabolisms. After 1 week, aromatic acids and phenols were extracted and analyzed by gas chromatography-mass spectrometry. [3',5'-2H2]Tyrosine produced 4-hydroxyphenyllactic acid, 4-hydroxyphenylpropionic acid and 4-hydroxyphenylacetic acid; [3',5'-2H2]-4'-hydroxyphenyllactic acid produced 4-hydroxyphenylpropionic acid, 3-hydroxyphenylpropionic acid, 4-hydroxyphenylacetic acid and phenylproionic acid; [3',5'-2H2]-4'-hydroxyphenyl-propionic acid produced 3-hydroxyphenylpropionic acid and phenylpropionic acid; [3',5',2,2-2H4]-4'-Hydroxyphenylacetic acid produced p-cresol; and [3',5'-2H2]-4'-hydroxybenzoic acid produced phenol. Thus the intestinal flora showed activities for decarboxylation leading to phenol and p-cresol, dehydroxylation leading to phenylpropionic acid and rearrangement leading to 3-hydroxyphenylpropionic acid. Rentention of both deuterium labels was observed in the rearrangement reaction.", "contents": "Study of the intestinal tyrosine metabolism using stable isotopes and gas chromatography-mass spectrometry. Deuterated tyrosine, 4-hydroxyphenyllactic acid, 4-hydroxyphenylpropionic acid, 4-hydroxyphenylacetic acid and 4-hydroxybenzoic acid were incubated under anaerobic conditions with human faecal specimens for the in vitro study of their respective metabolisms. After 1 week, aromatic acids and phenols were extracted and analyzed by gas chromatography-mass spectrometry. [3',5'-2H2]Tyrosine produced 4-hydroxyphenyllactic acid, 4-hydroxyphenylpropionic acid and 4-hydroxyphenylacetic acid; [3',5'-2H2]-4'-hydroxyphenyllactic acid produced 4-hydroxyphenylpropionic acid, 3-hydroxyphenylpropionic acid, 4-hydroxyphenylacetic acid and phenylproionic acid; [3',5'-2H2]-4'-hydroxyphenyl-propionic acid produced 3-hydroxyphenylpropionic acid and phenylpropionic acid; [3',5',2,2-2H4]-4'-Hydroxyphenylacetic acid produced p-cresol; and [3',5'-2H2]-4'-hydroxybenzoic acid produced phenol. Thus the intestinal flora showed activities for decarboxylation leading to phenol and p-cresol, dehydroxylation leading to phenylpropionic acid and rearrangement leading to 3-hydroxyphenylpropionic acid. Rentention of both deuterium labels was observed in the rearrangement reaction.", "PMID": 977696}
{"id": "pubmed23n0032_11275", "title": "Aldehyde condensation products as derivatives for the vapor phase analysis of biogenic amines.", "content": "The mass spectra of six tetrahydroisoquinolines and five tryptolines are surveyed. It is concluded that tryptolines are suitable for analysis of tryptamines, after condensation with aldehydes.", "contents": "Aldehyde condensation products as derivatives for the vapor phase analysis of biogenic amines. The mass spectra of six tetrahydroisoquinolines and five tryptolines are surveyed. It is concluded that tryptolines are suitable for analysis of tryptamines, after condensation with aldehydes.", "PMID": 977697}
{"id": "pubmed23n0032_11276", "title": "Applications of boronate derivatives in the study of ceramides by gas-liquid chromatography-mass spectrometry.", "content": "Methaneboronate derivatives of ceramides possess excellent gas-liquid chromatographic properties and give informative mass spectra. Molecular ions, present at high abundance where an unsaturated acyl substituent is present, are accompanied in the electron impact mass spectra by fragment ions which denote the acyl group and long-chain base. In the gas-liquid chromatographic-mass spectrometric analyses of natural ceramides, as methaneboronate derivatives, the properties of open-tubular columns are exploited to give good separations in moderate analysis times. An application is made to the analysis of ceramides derived from sphingomyelin of human arterial tissue.", "contents": "Applications of boronate derivatives in the study of ceramides by gas-liquid chromatography-mass spectrometry. Methaneboronate derivatives of ceramides possess excellent gas-liquid chromatographic properties and give informative mass spectra. Molecular ions, present at high abundance where an unsaturated acyl substituent is present, are accompanied in the electron impact mass spectra by fragment ions which denote the acyl group and long-chain base. In the gas-liquid chromatographic-mass spectrometric analyses of natural ceramides, as methaneboronate derivatives, the properties of open-tubular columns are exploited to give good separations in moderate analysis times. An application is made to the analysis of ceramides derived from sphingomyelin of human arterial tissue.", "PMID": 977698}
{"id": "pubmed23n0032_11277", "title": "Chemical ionization mass spectrometry for rapid assay of drugs in serum.", "content": "A rapid procedure has been devised for analyzing anti-epileptic drugs in serum by chemical ionization mass spectrometry. An internal standard, 5-(p-methylphenyl)-5-phenylhydantoin (MPPH), is added to serum diluted in buffer at pH 12. The mixture is washed with diethyl ether to remove neutral lipids, acidified, extracted with chloroform and the chloroform extract evaporated to dryness. The residue is then dissolved in methanol and an aliquot, corresponding to approximately 2% of the original mixture, is deposited in the glass capillary sample cup of the solid probe inlet of the mass spectrometer. The sample is then volatilized by heat into the ion source of the mass spectrometer, where it reacts with ionized methane reagent gas. As the temperature of the probe is increased, quasimolecular ion peaks of the protonated anticonvulsants appear, rise and fall on the oscilloscope tracing, indicating similar but not identical rates of volatilization. We recorded these peaks photographically by opening the shutter of the oscilloscope camera for the entire heating cycle. The concentrations of the anticonvulsants were estimated from the ratio of the height of the peaks of the drug to that of the internal standard on the photograph. The peak-height ratios were proportional to concentration within, above and below the therapeutic range. Other drugs, including barbiturates, carbamazepine, nicotine and caffeine, were readily identified when present. With one solid probe inlet, an assay could be performed every 2 min.", "contents": "Chemical ionization mass spectrometry for rapid assay of drugs in serum. A rapid procedure has been devised for analyzing anti-epileptic drugs in serum by chemical ionization mass spectrometry. An internal standard, 5-(p-methylphenyl)-5-phenylhydantoin (MPPH), is added to serum diluted in buffer at pH 12. The mixture is washed with diethyl ether to remove neutral lipids, acidified, extracted with chloroform and the chloroform extract evaporated to dryness. The residue is then dissolved in methanol and an aliquot, corresponding to approximately 2% of the original mixture, is deposited in the glass capillary sample cup of the solid probe inlet of the mass spectrometer. The sample is then volatilized by heat into the ion source of the mass spectrometer, where it reacts with ionized methane reagent gas. As the temperature of the probe is increased, quasimolecular ion peaks of the protonated anticonvulsants appear, rise and fall on the oscilloscope tracing, indicating similar but not identical rates of volatilization. We recorded these peaks photographically by opening the shutter of the oscilloscope camera for the entire heating cycle. The concentrations of the anticonvulsants were estimated from the ratio of the height of the peaks of the drug to that of the internal standard on the photograph. The peak-height ratios were proportional to concentration within, above and below the therapeutic range. Other drugs, including barbiturates, carbamazepine, nicotine and caffeine, were readily identified when present. With one solid probe inlet, an assay could be performed every 2 min.", "PMID": 977699}
{"id": "pubmed23n0032_11278", "title": "Structure-gas chromatographic electron capture sensitivity relationships of some substituted 17alpha-acetoxyprogesterones.", "content": "Structure-electron capture sensitivity relationships were established for underivatized 17alpha-acetoxyprogesterones. While progesterone was very insensitive, 17alpha-acetoxyprogesterone had a response of 4.8 X 10(2) C/mole. Methyl groups in the A or B ring of 17alpha-acetoxyprogesterone had no effect. A keto group at C-6 was 25 times more sensitive (1.2 X 10(4) C/mole). A double bond at C-6,7 enhanced the sensitivity sevenfold (3.5 X 10(3) C/mole), but double bonds at C-1,2 or C-9,11 had only slight effect. Substitution at C-16 was important. A methyl group at C-16 had two and three times the sensitivity in the 3-keto delta4 and 3-keto delta4,6 series (1.1 X 10(3) and 1.1 X 10(4) C/mole), respectively. A methylene group at C-16, in contrast showed a six-and twofold greater sensitivity over the C-16 methyl in the two series (7 X 10(3) and 2.2 X 10(4) C/mole), respectively. The most sensitive compound was 6-dehydro-6methyl-16-methylene-17alpha-acetoxyprogesterone (melengestrol acetate). Its sensitivity was 2.2 X 10(4) C/MOL, Comparable to the most sensitive halo esters of steroid alcohols reported in the literature. Its electron capture coefficient was 3-7.6X 10(10) 1/mole. The coef-icient was independent of the detector temperature, indicating low activation energy for electron absorpiton.", "contents": "Structure-gas chromatographic electron capture sensitivity relationships of some substituted 17alpha-acetoxyprogesterones. Structure-electron capture sensitivity relationships were established for underivatized 17alpha-acetoxyprogesterones. While progesterone was very insensitive, 17alpha-acetoxyprogesterone had a response of 4.8 X 10(2) C/mole. Methyl groups in the A or B ring of 17alpha-acetoxyprogesterone had no effect. A keto group at C-6 was 25 times more sensitive (1.2 X 10(4) C/mole). A double bond at C-6,7 enhanced the sensitivity sevenfold (3.5 X 10(3) C/mole), but double bonds at C-1,2 or C-9,11 had only slight effect. Substitution at C-16 was important. A methyl group at C-16 had two and three times the sensitivity in the 3-keto delta4 and 3-keto delta4,6 series (1.1 X 10(3) and 1.1 X 10(4) C/mole), respectively. A methylene group at C-16, in contrast showed a six-and twofold greater sensitivity over the C-16 methyl in the two series (7 X 10(3) and 2.2 X 10(4) C/mole), respectively. The most sensitive compound was 6-dehydro-6methyl-16-methylene-17alpha-acetoxyprogesterone (melengestrol acetate). Its sensitivity was 2.2 X 10(4) C/MOL, Comparable to the most sensitive halo esters of steroid alcohols reported in the literature. Its electron capture coefficient was 3-7.6X 10(10) 1/mole. The coef-icient was independent of the detector temperature, indicating low activation energy for electron absorpiton.", "PMID": 977700}
{"id": "pubmed23n0032_11279", "title": "Application of gas-liquid chromatography and high-performance liquid chromatography to the analysis of trace amounts of salicylic acid, acetylsalicylic anhydride and acetylsalicylsalicylic acid in aspirin samples and aspirin formulations.", "content": "The gas-liquid chromatographic (GLC) determination of salicylic acid (SA) in 12 commercial acetylsalicylic acid (aspirin, ASA) samples and 12 ASA formulations is reported. The GLC determination of SA as an impurity in ASA, utilising methylation with methyl iodide in the presence of potassium carbonate, requires a column chromatographic separation of SA prior to derivatization. Trace amounts of SA in ASA have also been determined by high-performance liquid chromatography (HPLC) on a Sil-X-I adsorption column using light petroleum-ethyl acetate-acetic acid as the mobile phase. Acetylsalicylic anhydride (ASN) and acetylsalicylsalicylic acid (ASSA) were determined by HPLC on a reversed-phase C18 column with water-methanol mixtures as the mobile phase. GLC was also applied to the determination of ASN as an impurity in ASA formulations.", "contents": "Application of gas-liquid chromatography and high-performance liquid chromatography to the analysis of trace amounts of salicylic acid, acetylsalicylic anhydride and acetylsalicylsalicylic acid in aspirin samples and aspirin formulations. The gas-liquid chromatographic (GLC) determination of salicylic acid (SA) in 12 commercial acetylsalicylic acid (aspirin, ASA) samples and 12 ASA formulations is reported. The GLC determination of SA as an impurity in ASA, utilising methylation with methyl iodide in the presence of potassium carbonate, requires a column chromatographic separation of SA prior to derivatization. Trace amounts of SA in ASA have also been determined by high-performance liquid chromatography (HPLC) on a Sil-X-I adsorption column using light petroleum-ethyl acetate-acetic acid as the mobile phase. Acetylsalicylic anhydride (ASN) and acetylsalicylsalicylic acid (ASSA) were determined by HPLC on a reversed-phase C18 column with water-methanol mixtures as the mobile phase. GLC was also applied to the determination of ASN as an impurity in ASA formulations.", "PMID": 977701}
{"id": "pubmed23n0032_11280", "title": "Post-column fluorescence derivatization of peptides. Problems and potential in high-performance liquid chromatography.", "content": "Some  critical parameters such as the pumping system, mixing devices and detector design in instrumentation for post-column derivatization in high-performance liquid chromatography are discussed. The derivatization was studied with pharmaceutically important nona-peptides containing primary amino groups which react with Fluram and reaction parameters such as pH, solvent and reagent concentration were investigated. Both adsorption systems and reversed-phase systems were used to separate the peptides prior to the post-column reaction. Reversed-phase chromatography has the advantage of simpler sample preparation, better reaction control and optimization of solvent conditions. As a result, detection limits of between 5 and 10 ng per injection can be obtained and the reproducibility of the results is better than +/- 2% (relative standard deviation). The method has been applied to the analysis of injection solutions (ampoules).", "contents": "Post-column fluorescence derivatization of peptides. Problems and potential in high-performance liquid chromatography. Some  critical parameters such as the pumping system, mixing devices and detector design in instrumentation for post-column derivatization in high-performance liquid chromatography are discussed. The derivatization was studied with pharmaceutically important nona-peptides containing primary amino groups which react with Fluram and reaction parameters such as pH, solvent and reagent concentration were investigated. Both adsorption systems and reversed-phase systems were used to separate the peptides prior to the post-column reaction. Reversed-phase chromatography has the advantage of simpler sample preparation, better reaction control and optimization of solvent conditions. As a result, detection limits of between 5 and 10 ng per injection can be obtained and the reproducibility of the results is better than +/- 2% (relative standard deviation). The method has been applied to the analysis of injection solutions (ampoules).", "PMID": 977702}
{"id": "pubmed23n0032_11281", "title": "Evaluation of microparticle chemically bonded reversed-phase packings in the high-pressure liquid chromatographic analysis of nucleosides and their bases.", "content": "The reversed-phase partition mode of high-pressure liquid chromatography was used for the analysis of seven of the naturally occurring nucleosides and their bases. With microparticle chemically bonded packings, nucleosides and their bases can be quantitatively determined in the presence of nucleotides in 30 min with high sensitivity, accuracy, and reproducibility. Peaks in chromatograms of cell extracts were identified by absorbance ratios and enzymatic peak shift methods. Applications of this technique to biochemical studies are reported.", "contents": "Evaluation of microparticle chemically bonded reversed-phase packings in the high-pressure liquid chromatographic analysis of nucleosides and their bases. The reversed-phase partition mode of high-pressure liquid chromatography was used for the analysis of seven of the naturally occurring nucleosides and their bases. With microparticle chemically bonded packings, nucleosides and their bases can be quantitatively determined in the presence of nucleotides in 30 min with high sensitivity, accuracy, and reproducibility. Peaks in chromatograms of cell extracts were identified by absorbance ratios and enzymatic peak shift methods. Applications of this technique to biochemical studies are reported.", "PMID": 977703}
{"id": "pubmed23n0032_11282", "title": "Selective liquid chromatographic isolation procedure for gas chromatographic-mass spectrometric analysis of 3-ketosteroids in biological materials.", "content": "A method for the isolation of 3-ketosteroids based on the positive charge of their oximes is described. The biological extract is filtered through a column of sulphoethyl Sephadex LH-20 (H+). Steroids in the filtrate are converted into oximes and the dried reaction mixture is filtered through a column of diethylaminohydroxypropyl Sephadex LH-20 (OH-) in methanol. After evaporation of the solvent and hydroxylamine, the oximes are taken up by and separated on a column of sulphoethyl Sephadex LH-20 (H+) in  methanol. Following elution of other steroids, the oximes of 3-ketosteroids are eluted as a group with methanol-pyridine (20:1, v/v), and are converted into trimethylsilyl ethers. Removal of reagents and further purification of the sample is achieved by rapid filtration through Lipidex 5000 in n-hexane-pyridine-hexamethyldisilazane-dimethoxypropane (97:1:2:10). The derivatives are then analyzed by computerized gas chromatography-mass spectrometry using open-tubular glass capillary columns. Recoveries of picogram amounts of 3H-labelled steroids carried through the entire isolation procedure are 80-90%. The purification achieved in analyses of plasma permits solid injection of the equivalent of 1-2 ml of plasma without overloading of the capillary column. The principle of the isolation procedure might be applicable to other groups of compounds that possess keto or aldehydo groups.", "contents": "Selective liquid chromatographic isolation procedure for gas chromatographic-mass spectrometric analysis of 3-ketosteroids in biological materials. A method for the isolation of 3-ketosteroids based on the positive charge of their oximes is described. The biological extract is filtered through a column of sulphoethyl Sephadex LH-20 (H+). Steroids in the filtrate are converted into oximes and the dried reaction mixture is filtered through a column of diethylaminohydroxypropyl Sephadex LH-20 (OH-) in methanol. After evaporation of the solvent and hydroxylamine, the oximes are taken up by and separated on a column of sulphoethyl Sephadex LH-20 (H+) in  methanol. Following elution of other steroids, the oximes of 3-ketosteroids are eluted as a group with methanol-pyridine (20:1, v/v), and are converted into trimethylsilyl ethers. Removal of reagents and further purification of the sample is achieved by rapid filtration through Lipidex 5000 in n-hexane-pyridine-hexamethyldisilazane-dimethoxypropane (97:1:2:10). The derivatives are then analyzed by computerized gas chromatography-mass spectrometry using open-tubular glass capillary columns. Recoveries of picogram amounts of 3H-labelled steroids carried through the entire isolation procedure are 80-90%. The purification achieved in analyses of plasma permits solid injection of the equivalent of 1-2 ml of plasma without overloading of the capillary column. The principle of the isolation procedure might be applicable to other groups of compounds that possess keto or aldehydo groups.", "PMID": 977704}
{"id": "pubmed23n0032_11283", "title": "Analysis of benzoylecgonine in urine by high-performance liquid chromatography and gas chromatography-mass spectrometry.", "content": "Benzoylecgonine, a polar metabolite of cocaine, was extracted and purified from urine using a reversed-phase high-performance liquid chromatographic column. The portion of the eluent corresponding to elution of the drug was collected, derivatized, and quantified using gas chromatography-mass spectrometry with selected ion monitoring. Using this procedure, analysis of 1 ng/ml of benzoylecgonine in urine can be achieved.", "contents": "Analysis of benzoylecgonine in urine by high-performance liquid chromatography and gas chromatography-mass spectrometry. Benzoylecgonine, a polar metabolite of cocaine, was extracted and purified from urine using a reversed-phase high-performance liquid chromatographic column. The portion of the eluent corresponding to elution of the drug was collected, derivatized, and quantified using gas chromatography-mass spectrometry with selected ion monitoring. Using this procedure, analysis of 1 ng/ml of benzoylecgonine in urine can be achieved.", "PMID": 977705}
{"id": "pubmed23n0032_11284", "title": "Microdetermination of nitrates and nitrites in saliva, blood, water, and suspended particulates in air by gas chromatography.", "content": "A generally applicable method for the analysis of nitrates and nitrites  has been used for a wide variety of samples, including human saliva, blood, drinking water, and airborne particulates. Aqueous nitrate ion is first converted to nitrobenzene by reaction with benzene (or another aromatic reactant) in the presence of a catalyst. The nitrobenzene is then quantitated by electron capture gas chromatography (GC-ECD). Nitrite ion and gaseous oxides of nitrogen can be determined also if such samples are treated appropriately prior to CG-ECD analysis. Other reactants such as 1,3,5-trimethoxybenzene can also be used in the place of benzene. The high sensitivity of ECD allows the quantitation of as little as 0.1 ppm (w/w) nitrate in a single drop of saliva or blood.", "contents": "Microdetermination of nitrates and nitrites in saliva, blood, water, and suspended particulates in air by gas chromatography. A generally applicable method for the analysis of nitrates and nitrites  has been used for a wide variety of samples, including human saliva, blood, drinking water, and airborne particulates. Aqueous nitrate ion is first converted to nitrobenzene by reaction with benzene (or another aromatic reactant) in the presence of a catalyst. The nitrobenzene is then quantitated by electron capture gas chromatography (GC-ECD). Nitrite ion and gaseous oxides of nitrogen can be determined also if such samples are treated appropriately prior to CG-ECD analysis. Other reactants such as 1,3,5-trimethoxybenzene can also be used in the place of benzene. The high sensitivity of ECD allows the quantitation of as little as 0.1 ppm (w/w) nitrate in a single drop of saliva or blood.", "PMID": 977706}
{"id": "pubmed23n0032_11285", "title": "Evaluation of organic pollutants in the open air and atmospheres in industrial sites using graphitized carbon black traps and gas chromatographic-mass spectrometric analysis with specific detectors.", "content": "A method for evaluating the organic content of the atmosphere, involving the use of personal samplers, is described. A comparison of the performances of Tenax GC and Carbopack B is given in terms of sample recovery. Apparatus including selective columns, specific detectors and combined gas chromatography-mass spectrometry is also described. Practical examples of the analysis of organic air pollutants trapped in the open air and the atmosphere in a chemical plant are discussed. A comparison of the results of complete gas chromatography and of the total hydrocarbon content is also made.", "contents": "Evaluation of organic pollutants in the open air and atmospheres in industrial sites using graphitized carbon black traps and gas chromatographic-mass spectrometric analysis with specific detectors. A method for evaluating the organic content of the atmosphere, involving the use of personal samplers, is described. A comparison of the performances of Tenax GC and Carbopack B is given in terms of sample recovery. Apparatus including selective columns, specific detectors and combined gas chromatography-mass spectrometry is also described. Practical examples of the analysis of organic air pollutants trapped in the open air and the atmosphere in a chemical plant are discussed. A comparison of the results of complete gas chromatography and of the total hydrocarbon content is also made.", "PMID": 977707}
{"id": "pubmed23n0032_11286", "title": "Gas chromatographic-mass spectrometric evaluation of exhaled tobacco smoke.", "content": "The impact of cigarette smoking on the distribution of organic substances in ambient air has been determined for the intermediate volatility range. A simple sampling procedure was employed, involving gas-solid adsorption onto an organic polymer followed by direct thermal elution onto a glass capillary column. Aliphatic and substituted aromatic hydrocarbons are predominant in urban atmospheres. Depending on location and weather conditions the total concentration of such volatiles can differ by as much as a factor of 20. This high background variation makes it difficult to analyze for trace substances with low odor threshold values, such as encountered in cigarette smoke. Standard cigarettes were smoked in a relatively small room, having no air filtration system. Air samples of approximately 3.51 were taken. The amount of volatiles added to air by cigarette smoking is unsignificant. Substances were analyzed and identified by gas chromatography and gas chromatography-mass spectrometry with glass capillary columns. Many compounds reported in cigarette smoke condensate have been confirmed.", "contents": "Gas chromatographic-mass spectrometric evaluation of exhaled tobacco smoke. The impact of cigarette smoking on the distribution of organic substances in ambient air has been determined for the intermediate volatility range. A simple sampling procedure was employed, involving gas-solid adsorption onto an organic polymer followed by direct thermal elution onto a glass capillary column. Aliphatic and substituted aromatic hydrocarbons are predominant in urban atmospheres. Depending on location and weather conditions the total concentration of such volatiles can differ by as much as a factor of 20. This high background variation makes it difficult to analyze for trace substances with low odor threshold values, such as encountered in cigarette smoke. Standard cigarettes were smoked in a relatively small room, having no air filtration system. Air samples of approximately 3.51 were taken. The amount of volatiles added to air by cigarette smoking is unsignificant. Substances were analyzed and identified by gas chromatography and gas chromatography-mass spectrometry with glass capillary columns. Many compounds reported in cigarette smoke condensate have been confirmed.", "PMID": 977708}
{"id": "pubmed23n0032_11287", "title": "Application of high-speed liquid chromatography to the analysis of polyoxyethylene surfactants and their decomposition products in industrial process waters.", "content": "Complementary high-speed liquid chromatographic techniques have been combined with infrared spectroscopy for the characterization and analysis of polyoxyethylene fatty acid surfactants and their decomposition products in industrial process waters. The combination of molecular sieve chromatography and infrared spectroscopy offers a selective and simple method for the analysis of trace concentrations (detection limit approx. 0.05-0.1 ppm) of these surfactants. Liquid-solid and reversed-phase chromatography have proven useful for the characterization and analysis of free fatty acids. Some of the parameters that affect the reversed-phase separation of fatty acids are discussed.", "contents": "Application of high-speed liquid chromatography to the analysis of polyoxyethylene surfactants and their decomposition products in industrial process waters. Complementary high-speed liquid chromatographic techniques have been combined with infrared spectroscopy for the characterization and analysis of polyoxyethylene fatty acid surfactants and their decomposition products in industrial process waters. The combination of molecular sieve chromatography and infrared spectroscopy offers a selective and simple method for the analysis of trace concentrations (detection limit approx. 0.05-0.1 ppm) of these surfactants. Liquid-solid and reversed-phase chromatography have proven useful for the characterization and analysis of free fatty acids. Some of the parameters that affect the reversed-phase separation of fatty acids are discussed.", "PMID": 977709}
{"id": "pubmed23n0032_11288", "title": "Gas chromatographic determination of hydrazoic acid.", "content": "A gas chromatographic procedure was developed for the determination of hydrazoic acid. Two possible columns were evaluated. The limits of detectability were 1.5 ng, or approximately 0.05%, in aqueous solution (w/v) and 2 mg per liter in air.", "contents": "Gas chromatographic determination of hydrazoic acid. A gas chromatographic procedure was developed for the determination of hydrazoic acid. Two possible columns were evaluated. The limits of detectability were 1.5 ng, or approximately 0.05%, in aqueous solution (w/v) and 2 mg per liter in air.", "PMID": 977710}
{"id": "pubmed23n0032_11289", "title": "Determination of aminophenol isomers by high-speed liquid chromatography.", "content": "The simultaneous quantitation of aminophenol isomers (p-, m- and o-aminophenol) and anilline, which with previously reported methods was difficult and/or complicated, has been performed using a high-speed liquid chromatographic method. The resolutions of chromatograms were appropriate for separation and quantitation, when analysis times were 20 minutes. Since the method is applicable in aqueous media, a successful quantitation of p- and o-aminophenol formed by cytochrome P-450 model systems was carried out.", "contents": "Determination of aminophenol isomers by high-speed liquid chromatography. The simultaneous quantitation of aminophenol isomers (p-, m- and o-aminophenol) and anilline, which with previously reported methods was difficult and/or complicated, has been performed using a high-speed liquid chromatographic method. The resolutions of chromatograms were appropriate for separation and quantitation, when analysis times were 20 minutes. Since the method is applicable in aqueous media, a successful quantitation of p- and o-aminophenol formed by cytochrome P-450 model systems was carried out.", "PMID": 977711}
{"id": "pubmed23n0032_11290", "title": "Theophylline and dyphylline levels in serum by liquid chromatography.", "content": "The widespread use of theophylline and dyphylline as bronchodilators in the treatment of acute and chronic obstructive airway disease, and recognition of the need to control the therapeutic drug level has created a demand for fast accurate measurement techniques. Measurement of theophylline levels by ultraviolet spectrophotometric techniques is hindered by interferences from barbiturates and caffeine, and thermal lability of the drug necessitates derivatization if gas chromatography is to be used. Because of these factors, a liquid chromatographic method has been developed which allows separation of the underviatized drug from interfering compounds, and quantitation of the theophylline in a single operation. In addition, the drug-containing fraction can be readily collected and subjected to mass spectral examination if there is any question as to its identify. The latter feature has particular significance in medico-legal cases.", "contents": "Theophylline and dyphylline levels in serum by liquid chromatography. The widespread use of theophylline and dyphylline as bronchodilators in the treatment of acute and chronic obstructive airway disease, and recognition of the need to control the therapeutic drug level has created a demand for fast accurate measurement techniques. Measurement of theophylline levels by ultraviolet spectrophotometric techniques is hindered by interferences from barbiturates and caffeine, and thermal lability of the drug necessitates derivatization if gas chromatography is to be used. Because of these factors, a liquid chromatographic method has been developed which allows separation of the underviatized drug from interfering compounds, and quantitation of the theophylline in a single operation. In addition, the drug-containing fraction can be readily collected and subjected to mass spectral examination if there is any question as to its identify. The latter feature has particular significance in medico-legal cases.", "PMID": 977712}
{"id": "pubmed23n0032_11291", "title": "The separation, identification, and quantitation of cannabinoids and their tau=butyldimethylsilyl, trimethylsilylacetate, and diethylphosphate derivatives using high-pressure liquid chromatography, gas-liquid chromatography, and mass spectrometry.", "content": "Methods for the separation, identification, and quantitation, of cannabinoids present in hashish have been developed. These methods include high-pressure liquid chromatographic and gas-liquid chromatographic separation of cannabinol, delta 9-tetrahydrocannabinol, delta 8-tetrahydrocannabinol, and cannabidiol as well as their tau-butyidimethylsilyl either and trimethylsilylacetate derivatives. The necessity for internal standards; the sensitivity of the HPLC method; and some mass spectral fragmentation pathways for the tau-butyldimethylsily, trimethylsilylacetate, and diethylphosphate derivatives are discussed.", "contents": "The separation, identification, and quantitation of cannabinoids and their tau=butyldimethylsilyl, trimethylsilylacetate, and diethylphosphate derivatives using high-pressure liquid chromatography, gas-liquid chromatography, and mass spectrometry. Methods for the separation, identification, and quantitation, of cannabinoids present in hashish have been developed. These methods include high-pressure liquid chromatographic and gas-liquid chromatographic separation of cannabinol, delta 9-tetrahydrocannabinol, delta 8-tetrahydrocannabinol, and cannabidiol as well as their tau-butyidimethylsilyl either and trimethylsilylacetate derivatives. The necessity for internal standards; the sensitivity of the HPLC method; and some mass spectral fragmentation pathways for the tau-butyldimethylsily, trimethylsilylacetate, and diethylphosphate derivatives are discussed.", "PMID": 977713}
{"id": "pubmed23n0032_11292", "title": "Studies of human diploid fibroblast growth. I. Responses of normal and hypopituitary cells to fibroblast growth factor, insulin, and serum.", "content": "We have assayed the growth stimulating activity of bovine insulin, fibroblast growth factor (FGF), and fetal bovine serum (FBS) in diploid human fibroblasts from normal and idiopathic hypopituitary donors. All three factors stimulated DNA synthesis in cells arrested by serum starvation. FGF was active at concentrations as low as 5 ng/ml with maximum effect at 100 ng/ml. FGF stimualted DNA synthesis at lower concentrations than did insulin and also produced a greater maximum response. Only serum was capable of supporting cell division and growth, but FGF accellerated this growth rate when it was added to serum-containing medium. Hydrocortisone, actinomycin D, and cycloheximide inhibit FGF stimulation. There was no significant difference between fibroblasts from normal and hypopituitary donors.", "contents": "Studies of human diploid fibroblast growth. I. Responses of normal and hypopituitary cells to fibroblast growth factor, insulin, and serum. We have assayed the growth stimulating activity of bovine insulin, fibroblast growth factor (FGF), and fetal bovine serum (FBS) in diploid human fibroblasts from normal and idiopathic hypopituitary donors. All three factors stimulated DNA synthesis in cells arrested by serum starvation. FGF was active at concentrations as low as 5 ng/ml with maximum effect at 100 ng/ml. FGF stimualted DNA synthesis at lower concentrations than did insulin and also produced a greater maximum response. Only serum was capable of supporting cell division and growth, but FGF accellerated this growth rate when it was added to serum-containing medium. Hydrocortisone, actinomycin D, and cycloheximide inhibit FGF stimulation. There was no significant difference between fibroblasts from normal and hypopituitary donors.", "PMID": 977719}
{"id": "pubmed23n0032_11293", "title": "Thyrotoxic periodic paralysis: a peculiar case with unusual dystonic behavior and variable relations of paralysis to serum potassium levels.", "content": "This report describes a male patient, aged 49, with tyrotoxic periodic paralysis. The patient had had episodes of main d'accoucheur for eight years. Since thyrotoxicosis had affected the patient last year, he had had attacks of flaccid paralysis of the limbs associated with main d'accoucheur. While the spontaneous attack was normokalemic and responded favorably to potassium, attacks similar to the spontaneous one were provoked not only by glucose infusion, carbohydrate feeding, and NaCl infusion, but also by oral KCl administration. Transition from hypokalemic to hyperkalemic type of paralysis occurred during potassium treatment of a sodium-induced attack, and that from hyperkalemic to hypokalemic type of paralysis occurred after glucose infusion given during a potassium-induced attack. Intra-arterial epinephrine injection caused prompt paralysis of the perfused hand. A main d'accoucheur was induced in one hand by cooling it in chilled water. The spontaneous attacks of flaccid paralysis disappeared after the patient returned to the euthyroid state, but reappeared when he was treated with high doses of desiccated thyroid. Even when the patient was relieved from thyrotoxicosis,  he still experienced stiffness in his hands. The administration of glucose, insulin, and KCl also provoked attacks of paralysis with main d'accoucheur or main d'accoucheur. The disease could be of the normokalemic variety of periodic paralysis with fluctations in the potassium levels depending on the provocative tests employed. It seems that the unusual dystonic behavior of this patient is due not to myotonia, but to muscle spasm resulting from metabolic abnormalities. It could also be suggested that excess thyroid hormones have adverse effects on the development and syndrome of periodic paralysis by abetting a latent hereditary abnormality.", "contents": "Thyrotoxic periodic paralysis: a peculiar case with unusual dystonic behavior and variable relations of paralysis to serum potassium levels. This report describes a male patient, aged 49, with tyrotoxic periodic paralysis. The patient had had episodes of main d'accoucheur for eight years. Since thyrotoxicosis had affected the patient last year, he had had attacks of flaccid paralysis of the limbs associated with main d'accoucheur. While the spontaneous attack was normokalemic and responded favorably to potassium, attacks similar to the spontaneous one were provoked not only by glucose infusion, carbohydrate feeding, and NaCl infusion, but also by oral KCl administration. Transition from hypokalemic to hyperkalemic type of paralysis occurred during potassium treatment of a sodium-induced attack, and that from hyperkalemic to hypokalemic type of paralysis occurred after glucose infusion given during a potassium-induced attack. Intra-arterial epinephrine injection caused prompt paralysis of the perfused hand. A main d'accoucheur was induced in one hand by cooling it in chilled water. The spontaneous attacks of flaccid paralysis disappeared after the patient returned to the euthyroid state, but reappeared when he was treated with high doses of desiccated thyroid. Even when the patient was relieved from thyrotoxicosis,  he still experienced stiffness in his hands. The administration of glucose, insulin, and KCl also provoked attacks of paralysis with main d'accoucheur or main d'accoucheur. The disease could be of the normokalemic variety of periodic paralysis with fluctations in the potassium levels depending on the provocative tests employed. It seems that the unusual dystonic behavior of this patient is due not to myotonia, but to muscle spasm resulting from metabolic abnormalities. It could also be suggested that excess thyroid hormones have adverse effects on the development and syndrome of periodic paralysis by abetting a latent hereditary abnormality.", "PMID": 977720}
{"id": "pubmed23n0032_11294", "title": "Aromatization of androstenedione by cultured human fibroblasts.", "content": "The conversion of (1, 2, 6, 7-3H)androstenedione to (3H)estrone was measured in fibroblast monolayers grown from biopsies of genital and nongenital skins obtained from 15 control subjects, 9 males with developmental defects of the urogenital tract, and 8 patients with hereditary male pseudohermaphroditism. Under the standardized conditions utilized in this study, the rate of estrone formation in the fibroblasts from normal controls varied from less than 0.2 to 5.5 pmol/100 mg protein/h, and these rates were enhanced by incubation of intact monolayers with choleragen, theophylline, or dexamethasone. Rates of estrone formation were higher in some foreskin strains grown from subjects with developmental defects of the urogenital tract and in strains from scrotum and foreskin  of patients with familial incomplete male pseudohermaphroditism, types 1 and 2 than in normal strains or strains from patients with testicular feminization. The meaning of these apparent high rates of estrone formation is unclear.", "contents": "Aromatization of androstenedione by cultured human fibroblasts. The conversion of (1, 2, 6, 7-3H)androstenedione to (3H)estrone was measured in fibroblast monolayers grown from biopsies of genital and nongenital skins obtained from 15 control subjects, 9 males with developmental defects of the urogenital tract, and 8 patients with hereditary male pseudohermaphroditism. Under the standardized conditions utilized in this study, the rate of estrone formation in the fibroblasts from normal controls varied from less than 0.2 to 5.5 pmol/100 mg protein/h, and these rates were enhanced by incubation of intact monolayers with choleragen, theophylline, or dexamethasone. Rates of estrone formation were higher in some foreskin strains grown from subjects with developmental defects of the urogenital tract and in strains from scrotum and foreskin  of patients with familial incomplete male pseudohermaphroditism, types 1 and 2 than in normal strains or strains from patients with testicular feminization. The meaning of these apparent high rates of estrone formation is unclear.", "PMID": 977721}
{"id": "pubmed23n0032_11295", "title": "Propionic acidemia and hyperlysinemia in a case with ornithine transcarbamylase (OTC) deficiency.", "content": "A female infant with episodic hyperammonemia due to a disorder of the urea cycle and who had hyperlysinemia and an unusual elevation of short chain fatty acids, mainly propionate, is described. Both occurred apparently only during attacks of hyperammonemia. Propionic acidemia was ruled out by enzyme studies. OTC deficiency was diagnosed on the basis of: 1) decreased enzyme activity in leukocytes;2) hyperammonemia in response to protein intakes in excess of 2.0 g/kg/day; 3) orotic aciduria in the patient and her asymptomatic mother; 4) suggestive evidence of x-linked dominant inheritance; and 5) exclusion of citrullinemia, argininosuccinic aciduria, argininemia, and disorders of lysine metabolism that are associated with hyperammonemia. Homocitrullinuria, presence of epsilon-N-acetyl-l-lysine in urine, and absence of saccharopine indicate deficiency of the saccharopine pathway of lysine degradation. However, alpha-ketoglutarate reductase was normal in fibroblasts. Since these metabolites were observed only in conjunction with hyperammonemia but not after a lysine load, we suggest that there was competition between ammonia and lysine for alpha-ketoglutarate. The link between disorders of the urea cycle and short chain fatty acid metabolism remains unexplained..", "contents": "Propionic acidemia and hyperlysinemia in a case with ornithine transcarbamylase (OTC) deficiency. A female infant with episodic hyperammonemia due to a disorder of the urea cycle and who had hyperlysinemia and an unusual elevation of short chain fatty acids, mainly propionate, is described. Both occurred apparently only during attacks of hyperammonemia. Propionic acidemia was ruled out by enzyme studies. OTC deficiency was diagnosed on the basis of: 1) decreased enzyme activity in leukocytes;2) hyperammonemia in response to protein intakes in excess of 2.0 g/kg/day; 3) orotic aciduria in the patient and her asymptomatic mother; 4) suggestive evidence of x-linked dominant inheritance; and 5) exclusion of citrullinemia, argininosuccinic aciduria, argininemia, and disorders of lysine metabolism that are associated with hyperammonemia. Homocitrullinuria, presence of epsilon-N-acetyl-l-lysine in urine, and absence of saccharopine indicate deficiency of the saccharopine pathway of lysine degradation. However, alpha-ketoglutarate reductase was normal in fibroblasts. Since these metabolites were observed only in conjunction with hyperammonemia but not after a lysine load, we suggest that there was competition between ammonia and lysine for alpha-ketoglutarate. The link between disorders of the urea cycle and short chain fatty acid metabolism remains unexplained..", "PMID": 977722}
{"id": "pubmed23n0032_11296", "title": "Improving the diagnostic reliability of rapidly fluctuating plasma hormone levels by optimized multiple-sampling techniques.", "content": "When plasma hormone levels undergo rapid and large oscillations, as in the case of testosterone, FSH, and LH, a single random sample is likely to yield a result within +/-20% of the true mean value only 68%, 54%, and 30% of the time, respectively. Multiple sampling increases reliability, and computer analysis demonstrates that three equally-spaced samples taken at 6 to 18 min intervals provide the optimum schedule, given certain practical considerations. Pooling of the three plasma samples prior to radioimmunoassay avoids an increased laboratory workload.", "contents": "Improving the diagnostic reliability of rapidly fluctuating plasma hormone levels by optimized multiple-sampling techniques. When plasma hormone levels undergo rapid and large oscillations, as in the case of testosterone, FSH, and LH, a single random sample is likely to yield a result within +/-20% of the true mean value only 68%, 54%, and 30% of the time, respectively. Multiple sampling increases reliability, and computer analysis demonstrates that three equally-spaced samples taken at 6 to 18 min intervals provide the optimum schedule, given certain practical considerations. Pooling of the three plasma samples prior to radioimmunoassay avoids an increased laboratory workload.", "PMID": 977723}
{"id": "pubmed23n0032_11297", "title": "Dose-response of prolactin and thyrotropin to N3im-methyl-thyrotropin releasing hormone in euthyroid men.", "content": "The synthetic N3im-methyl analogue of thyrotropin releasing hormone (methyl-TRH) was administered intravenously to 15 euthyroid men, ages 36-62, in graded doses from 6.25 mug to 500 mug in order to establish the range of response of prolactin (PRL), TSH, T3 and T4 to various doses of methyl-TRH. There was a dose-related rise in serum TSH, PRL, T3, and T4 which gave a nearly linear relationship when the integrated area of response was used as an index of response to the various doses of methyl-TRH. All 15 men had a clear elevation in TSH, PRL, T3 and T4 following the lowest dose of methyl-TRH TESTED (6.25 mug). There was considerable variability in the response to methyl-TRH among the individuals. One hundred mug of methyl-TRH gave a maximum TSH response; a 25 mug dose elicited a maximum PRL response.", "contents": "Dose-response of prolactin and thyrotropin to N3im-methyl-thyrotropin releasing hormone in euthyroid men. The synthetic N3im-methyl analogue of thyrotropin releasing hormone (methyl-TRH) was administered intravenously to 15 euthyroid men, ages 36-62, in graded doses from 6.25 mug to 500 mug in order to establish the range of response of prolactin (PRL), TSH, T3 and T4 to various doses of methyl-TRH. There was a dose-related rise in serum TSH, PRL, T3, and T4 which gave a nearly linear relationship when the integrated area of response was used as an index of response to the various doses of methyl-TRH. All 15 men had a clear elevation in TSH, PRL, T3 and T4 following the lowest dose of methyl-TRH TESTED (6.25 mug). There was considerable variability in the response to methyl-TRH among the individuals. One hundred mug of methyl-TRH gave a maximum TSH response; a 25 mug dose elicited a maximum PRL response.", "PMID": 977724}
{"id": "pubmed23n0032_11298", "title": "Serum lipid changes during contraceptive administration in obese women: relation to serum insulin levels.", "content": "Previous reports agree that estrogen and estrogen-containing contraceptives increase serum triglyceride levels of normal women, but disagree on their effect on serum cholesterol levels. Since obesity is often accompanied by hyperinsulinemia and since hyperinsulinemia may participate in production of hypertriglyceridemia, we investigated the effect of oral contraceptives on the serum lipids of obese women. Serum triglycerides and cholesterol were measured before and after 3 months administration of the contraceptives. The mean triglyceride level increased 23% in the obese and 21% in the normal women. The mean cholesterol level increased 6% (P less than 0.05) in the obese and did not change in the normal women. The increase in cholesterol occurred mostly in those with initial levels less than 225 mg/100 ml; in those with initial levels above 250 mg/100 ml the level usually decreased with treatment. The serum lipid changes were not related to the serum insulin levels.", "contents": "Serum lipid changes during contraceptive administration in obese women: relation to serum insulin levels. Previous reports agree that estrogen and estrogen-containing contraceptives increase serum triglyceride levels of normal women, but disagree on their effect on serum cholesterol levels. Since obesity is often accompanied by hyperinsulinemia and since hyperinsulinemia may participate in production of hypertriglyceridemia, we investigated the effect of oral contraceptives on the serum lipids of obese women. Serum triglycerides and cholesterol were measured before and after 3 months administration of the contraceptives. The mean triglyceride level increased 23% in the obese and 21% in the normal women. The mean cholesterol level increased 6% (P less than 0.05) in the obese and did not change in the normal women. The increase in cholesterol occurred mostly in those with initial levels less than 225 mg/100 ml; in those with initial levels above 250 mg/100 ml the level usually decreased with treatment. The serum lipid changes were not related to the serum insulin levels.", "PMID": 977725}
{"id": "pubmed23n0032_11299", "title": "Endogenous estrogen modulates phenothiazine stimulated prolactin secretion.", "content": "The role of endogenous estrogen in the regulation of serum prolactin concentration in man is controversial. To evaluate the possible effect of endogenous fluctuations in serum estrogen on the regulation of prolactin secretion, the authors determined phenothiazine stimulated prolactin secretion in 12 women in the early follicular phase of the menstrual cycle when estrogen levels were low (mean +/- SE E1 + E2 = 82 +/- 7 pg/ml) and compared it to the response during the late follicular phase when estrogen levels were higher (mean E1 + E2 = 320 +/- 63 pg/ml). Mean basal serum prolactin concentrations were similar in the early and late follicular phases of the cylcle (17 +/- 4 and 20 +/- 2 ng/ml, respectively). The integrated prolactin response following phenothiazine administration was significantly higher at mid-cycle (402 +/- 46 ng-hr/ml) than in the early follicular phase (317 +/0 46 ng-hr/ml, P less than .02). Thus, these studies suggest that endogenous estrogen secretion may play a role in the regulation of serum prolactin concentration in man.", "contents": "Endogenous estrogen modulates phenothiazine stimulated prolactin secretion. The role of endogenous estrogen in the regulation of serum prolactin concentration in man is controversial. To evaluate the possible effect of endogenous fluctuations in serum estrogen on the regulation of prolactin secretion, the authors determined phenothiazine stimulated prolactin secretion in 12 women in the early follicular phase of the menstrual cycle when estrogen levels were low (mean +/- SE E1 + E2 = 82 +/- 7 pg/ml) and compared it to the response during the late follicular phase when estrogen levels were higher (mean E1 + E2 = 320 +/- 63 pg/ml). Mean basal serum prolactin concentrations were similar in the early and late follicular phases of the cylcle (17 +/- 4 and 20 +/- 2 ng/ml, respectively). The integrated prolactin response following phenothiazine administration was significantly higher at mid-cycle (402 +/- 46 ng-hr/ml) than in the early follicular phase (317 +/0 46 ng-hr/ml, P less than .02). Thus, these studies suggest that endogenous estrogen secretion may play a role in the regulation of serum prolactin concentration in man.", "PMID": 977726}
{"id": "pubmed23n0032_11300", "title": "Partial characterization of somatomedin bioactivity in term human amniotic fluid.", "content": "Somatomedin (SM) activity in human amniotic fluid (AF) was partially characterized. Aliquots of pooled AF, obtained at term gestation, were acidified to pH 2.3 and sequentially studied by ultrafiltration on various membranes, Sephadex gel filtration, and starch gel electrophoresis (SGE). SM activity was measured by a rat cartilage bioassay. SM activity in AF, under these conditions, was present in several stable indicated molecular size (IMS) forms; large (greater than 45,000) and small (near 10,000). The small SM was found in basic and acidic forms after SGE. These studies suggest that SM activity in term human AF is somewhat similar to SM in unextracted plasma. In addition, immunoreactive GH and insulin were measured in AF but at lower concentrations than usually found in plasma. After chromatography of AF, GH was found in large and small IMS forms, whereas most of the insulin was found in the monomer insulin IMS area.", "contents": "Partial characterization of somatomedin bioactivity in term human amniotic fluid. Somatomedin (SM) activity in human amniotic fluid (AF) was partially characterized. Aliquots of pooled AF, obtained at term gestation, were acidified to pH 2.3 and sequentially studied by ultrafiltration on various membranes, Sephadex gel filtration, and starch gel electrophoresis (SGE). SM activity was measured by a rat cartilage bioassay. SM activity in AF, under these conditions, was present in several stable indicated molecular size (IMS) forms; large (greater than 45,000) and small (near 10,000). The small SM was found in basic and acidic forms after SGE. These studies suggest that SM activity in term human AF is somewhat similar to SM in unextracted plasma. In addition, immunoreactive GH and insulin were measured in AF but at lower concentrations than usually found in plasma. After chromatography of AF, GH was found in large and small IMS forms, whereas most of the insulin was found in the monomer insulin IMS area.", "PMID": 977727}
{"id": "pubmed23n0032_11301", "title": "Suppression of luteinizing hormone in castrated women by the administration of human chorionic gonadotropin.", "content": "The suppressive effect of human chorionic gonadotropin (hCG) on luteinizing hormone (LH) and/or LH-beta was studied by specific LH-beta radioimmunoassay following hCG administration. Eight castrated women were each administered 10,000 IU of hCG in a single intramuscular injection and five women in the control group were injected with saline. The serum level of hCG increased after the injection, reaching 217.6 mIU/ml after 8 h. There was a significant suppression of LH levels as compared to those of the control group and the pre-injection levels: 68.2% 1 h after injection, 64.7% after 2h, 65.5% after 4 h, 77.0% after 8 h, 78.6% after 12 h, and 78.2% after 24 h. There was no significant suppression of the follicle-stimulating hormone (FSH) as compared to the preinjection and control values. Serum concentration of estradiol1 was not detectable either before or after the hCG injection. We conclude that hCG has a suppressive effect on LH and/or LH-beta secretion not mediated by estradiol.", "contents": "Suppression of luteinizing hormone in castrated women by the administration of human chorionic gonadotropin. The suppressive effect of human chorionic gonadotropin (hCG) on luteinizing hormone (LH) and/or LH-beta was studied by specific LH-beta radioimmunoassay following hCG administration. Eight castrated women were each administered 10,000 IU of hCG in a single intramuscular injection and five women in the control group were injected with saline. The serum level of hCG increased after the injection, reaching 217.6 mIU/ml after 8 h. There was a significant suppression of LH levels as compared to those of the control group and the pre-injection levels: 68.2% 1 h after injection, 64.7% after 2h, 65.5% after 4 h, 77.0% after 8 h, 78.6% after 12 h, and 78.2% after 24 h. There was no significant suppression of the follicle-stimulating hormone (FSH) as compared to the preinjection and control values. Serum concentration of estradiol1 was not detectable either before or after the hCG injection. We conclude that hCG has a suppressive effect on LH and/or LH-beta secretion not mediated by estradiol.", "PMID": 977728}
{"id": "pubmed23n0032_11302", "title": "Methylphenidate increases serum growth hormone concentrations.", "content": "Serum growth hormone (GH) and cortisol concentrations were measured in 12 healthy, young men following oral administration of methylphenidate (20 mgm ) or a placebo. Methylphenidate elicited unequivocal GH elevations in 9 of 12 subjects while only 2 subjects showed GH elevation following placebo. Cortisol concentrations were not affected by methylphenidate.", "contents": "Methylphenidate increases serum growth hormone concentrations. Serum growth hormone (GH) and cortisol concentrations were measured in 12 healthy, young men following oral administration of methylphenidate (20 mgm ) or a placebo. Methylphenidate elicited unequivocal GH elevations in 9 of 12 subjects while only 2 subjects showed GH elevation following placebo. Cortisol concentrations were not affected by methylphenidate.", "PMID": 977729}
{"id": "pubmed23n0032_11303", "title": "Biological activity of des-Asp1-,Ileu8-angiotensin II (Ileu8-angiotensin III) in man.", "content": "Biological activity of ileu8-angiotensin III (AIIIA) was studied in man. In 5 normal men intravenous infusion of 200 ng/kg/min of AIIIA for 30 minutes from 0900 h had no effect on blood pressure (BP) but caused a decrease in plasma renin activity (PRA) and an increase in plasma aldosterone (PA). This dose did not inhibit pressor and steroidogenic actions of angiotensin II (AII) infused into the normal men at a rate of 20 ng/kg/min for 30 minutes. In 3 patients with Bartter's syndrome 260-1,200 ng/kg/ min of AIIIA infusion for 30 minutes from 0900 h had no effect on BP but caused decreases in PRA and PA. These results indicate that in man AIIIA has no pressor action and no antagonistic effect on pressor action of AII but has PRA-lowering and aldosterone-stimulating effects. Antagonistic effect of AIIIA on steroidogenic action of AII was also shown in patients with Bartter's syndrome but not in AII-treated normal men. This may be due to the difference of administered dose of AIIIA.", "contents": "Biological activity of des-Asp1-,Ileu8-angiotensin II (Ileu8-angiotensin III) in man. Biological activity of ileu8-angiotensin III (AIIIA) was studied in man. In 5 normal men intravenous infusion of 200 ng/kg/min of AIIIA for 30 minutes from 0900 h had no effect on blood pressure (BP) but caused a decrease in plasma renin activity (PRA) and an increase in plasma aldosterone (PA). This dose did not inhibit pressor and steroidogenic actions of angiotensin II (AII) infused into the normal men at a rate of 20 ng/kg/min for 30 minutes. In 3 patients with Bartter's syndrome 260-1,200 ng/kg/ min of AIIIA infusion for 30 minutes from 0900 h had no effect on BP but caused decreases in PRA and PA. These results indicate that in man AIIIA has no pressor action and no antagonistic effect on pressor action of AII but has PRA-lowering and aldosterone-stimulating effects. Antagonistic effect of AIIIA on steroidogenic action of AII was also shown in patients with Bartter's syndrome but not in AII-treated normal men. This may be due to the difference of administered dose of AIIIA.", "PMID": 977730}
{"id": "pubmed23n0032_11304", "title": "Study on the relationship between plasma prolactin levels and androgen metabolism in man.", "content": "The purpose of the study was to investigate the effect of hyperprolactinemia on the metabolism of androgens in man. A group of 6 normal men was treated for 4 consecutive days, on separate periods, with Sulpiride which is known to raise plasma prolactin (PRL) concentration. The effect of the treatment on plasma steroids was verified in basal conditions and under stimulation by HCG. In the controls, a parallel rise in testosterone (T) and dihydrotestosterone (DHT) was observed in response to HCG stimulation. In experimental hyperprolactinemia, the rise in T in response to HCG, similar to that of the controls, was accompanied by a markedly diminished rise in DHT. Similar results were observed in a patient with hyperprolactinemia following apparent accidental section of the pituitary stalk. These data demonstrate the interference of increased levels of PRL in the metabolism of testosterone into the active DHT form by 5alpha-reductase. They suggest that this mechanism could possibly impair the gonadal function in man.", "contents": "Study on the relationship between plasma prolactin levels and androgen metabolism in man. The purpose of the study was to investigate the effect of hyperprolactinemia on the metabolism of androgens in man. A group of 6 normal men was treated for 4 consecutive days, on separate periods, with Sulpiride which is known to raise plasma prolactin (PRL) concentration. The effect of the treatment on plasma steroids was verified in basal conditions and under stimulation by HCG. In the controls, a parallel rise in testosterone (T) and dihydrotestosterone (DHT) was observed in response to HCG stimulation. In experimental hyperprolactinemia, the rise in T in response to HCG, similar to that of the controls, was accompanied by a markedly diminished rise in DHT. Similar results were observed in a patient with hyperprolactinemia following apparent accidental section of the pituitary stalk. These data demonstrate the interference of increased levels of PRL in the metabolism of testosterone into the active DHT form by 5alpha-reductase. They suggest that this mechanism could possibly impair the gonadal function in man.", "PMID": 977731}
{"id": "pubmed23n0032_11305", "title": "Quantitative and bacteriological studies of urine specimens from canine and feline urinary tract infections.", "content": "The most prevalent microorganisms isolated from urine specimens of dogs (385) and cats (132) with clinical signs of urinary tract infections were Escherichia coli, Proteus spp., and Staphylococcus aureus. The results of quantitative urine-culturing methods showed 48.6% of the canine and 12.1% of the feline specimens had more than 10(5) organisms per ml of urine. The bacteria isolated appear to have a greater resistance to antibacterial agents than previously reported.", "contents": "Quantitative and bacteriological studies of urine specimens from canine and feline urinary tract infections. The most prevalent microorganisms isolated from urine specimens of dogs (385) and cats (132) with clinical signs of urinary tract infections were Escherichia coli, Proteus spp., and Staphylococcus aureus. The results of quantitative urine-culturing methods showed 48.6% of the canine and 12.1% of the feline specimens had more than 10(5) organisms per ml of urine. The bacteria isolated appear to have a greater resistance to antibacterial agents than previously reported.", "PMID": 977732}
{"id": "pubmed23n0032_11306", "title": "Growth requirements of some small-colony-forming variants of Staphylococcus aureus.", "content": "Nine stable, auxotrophic, small-colony-forming variants of Staphylococcus aureus were isolated. They were of two types. One type, from broth cultures containing varying concentrations of gentamicin or kanamycin, required heme for normal growth. The second type, from the blood culture of a patient who had been treated with penicillin and oxacillin, required long-chain unsaturated fatty acids for normal growth. These small-colony-forming variants differed from their parents with respect to specific metabolic activities, many of which may be attributed to an impaired electron transport system and related functions. A laboratory procedure for detecting possible small-colony variants in clinical specimens was proposed.", "contents": "Growth requirements of some small-colony-forming variants of Staphylococcus aureus. Nine stable, auxotrophic, small-colony-forming variants of Staphylococcus aureus were isolated. They were of two types. One type, from broth cultures containing varying concentrations of gentamicin or kanamycin, required heme for normal growth. The second type, from the blood culture of a patient who had been treated with penicillin and oxacillin, required long-chain unsaturated fatty acids for normal growth. These small-colony-forming variants differed from their parents with respect to specific metabolic activities, many of which may be attributed to an impaired electron transport system and related functions. A laboratory procedure for detecting possible small-colony variants in clinical specimens was proposed.", "PMID": 977733}
{"id": "pubmed23n0032_11307", "title": "Evaluation of commercial Salmonella O polyvalent and Vi antisera.", "content": "Salmonella somatic polyvalent antisera for groups A through E or A through I with or without Vi and Salmonella Vi antisera were purchased from four commercial producers and evaluated. Center for Disease Control (CDC) methods and specifications were used. The four polyvalent and two of the four Vi antisera did not meet CDC performance specifications. Suggestions are made for improving the laboratory usefulness of the reagents and the product labeling.", "contents": "Evaluation of commercial Salmonella O polyvalent and Vi antisera. Salmonella somatic polyvalent antisera for groups A through E or A through I with or without Vi and Salmonella Vi antisera were purchased from four commercial producers and evaluated. Center for Disease Control (CDC) methods and specifications were used. The four polyvalent and two of the four Vi antisera did not meet CDC performance specifications. Suggestions are made for improving the laboratory usefulness of the reagents and the product labeling.", "PMID": 977734}
{"id": "pubmed23n0032_11308", "title": "A new approach to psychopathology.", "content": "A new approach to psychopathology based on the psychology of integration as applied to psychological states is outlined. Because all raw behavior occurs only in the form of psychological states, all formulations of psychopathology must refer to integrative disorders that are postulated to underlie all pathological or defective behaviors. Disintegration is the one common factor found in all mental disorders. Lack of integration underlies most inadaptability. The classical field of psychopathology, which formerly was limited to the consideration of formal psychiatric disorders, now is expanded to include deficits, imbalances and disintegrations of acute or chornic nature that result in erroneous or less than perfect judgments. Judgment is considered to reflect the quality of underlying integrative processes. Judgmental defects that lead to maladaptive decisions inevitably must result in maladjustment and/or less than perfect performance. It is necessary to differentiate between (a) integrative disorders per se, and (b) the personal-social effects of positive or negative integrations organized by healthy or unhealthy factors. Criminals may be well integrated in their asocial activities, but socially undesirable because organized about the criminal ethic. Postulates are presented that outline the theoretical assumptions upon which integrative psychopathology is based. This approach utilizes classic theories such as the Freudian psychopathology of everyday life when valid and relevant within the larger framework of integrative psychology. A differentiation is made between hierarchical levels of increasingly complex factors that may organize integration.", "contents": "A new approach to psychopathology. A new approach to psychopathology based on the psychology of integration as applied to psychological states is outlined. Because all raw behavior occurs only in the form of psychological states, all formulations of psychopathology must refer to integrative disorders that are postulated to underlie all pathological or defective behaviors. Disintegration is the one common factor found in all mental disorders. Lack of integration underlies most inadaptability. The classical field of psychopathology, which formerly was limited to the consideration of formal psychiatric disorders, now is expanded to include deficits, imbalances and disintegrations of acute or chornic nature that result in erroneous or less than perfect judgments. Judgment is considered to reflect the quality of underlying integrative processes. Judgmental defects that lead to maladaptive decisions inevitably must result in maladjustment and/or less than perfect performance. It is necessary to differentiate between (a) integrative disorders per se, and (b) the personal-social effects of positive or negative integrations organized by healthy or unhealthy factors. Criminals may be well integrated in their asocial activities, but socially undesirable because organized about the criminal ethic. Postulates are presented that outline the theoretical assumptions upon which integrative psychopathology is based. This approach utilizes classic theories such as the Freudian psychopathology of everyday life when valid and relevant within the larger framework of integrative psychology. A differentiation is made between hierarchical levels of increasingly complex factors that may organize integration.", "PMID": 977735}
{"id": "pubmed23n0032_11309", "title": "Recall of parental behaviors in female neurotic depressives.", "content": "Twenty-four neurotic depressive females and 24 female anxiety neurotics were compared on a 90-item questionnaire that concerned recall of parental behavior. Relative to anxiety neurotics, the neurotic depressives recalled fathers as unloving disciplinarians and recalled mothers as difficult to please, intrusive and controlling, and possibly more concerned with their own than with their children's needs. Previous findings that parents of depressives are recalled as depriving were supported, and a methodology for differentiating neurotic depressives from anxiety neurotics was described.", "contents": "Recall of parental behaviors in female neurotic depressives. Twenty-four neurotic depressive females and 24 female anxiety neurotics were compared on a 90-item questionnaire that concerned recall of parental behavior. Relative to anxiety neurotics, the neurotic depressives recalled fathers as unloving disciplinarians and recalled mothers as difficult to please, intrusive and controlling, and possibly more concerned with their own than with their children's needs. Previous findings that parents of depressives are recalled as depriving were supported, and a methodology for differentiating neurotic depressives from anxiety neurotics was described.", "PMID": 977736}
{"id": "pubmed23n0032_11310", "title": "The relationship of self-disclosure to personality, adjustment and self-actualization.", "content": "This study tested the hypothesis that a high level of self-disclosure is indicative of psychological adjustment and self-actualization. The self-disclosure scale, Social Avoidance and Distress, Fear of Negative Evaluation, Alienation and Repression-Sensitization scales were administered to 60 Ss. The results for all scales indicated that a high level of disclosure was associated with adjustment as measured by these scales. A second group of 94 Ss completed the self-disclosure scale and the Personal Orientation Inventory, a measure of self-actualization. The results indicated that high disclosers compared to low disclosers had achieved a higher level of self-actualization. That is, there were significant correlations between self-disclosure and measures of self-actualization such as Time Competence, Internal Support, Existentiality, Self-Acceptance and Capacity for Intimate Contact.", "contents": "The relationship of self-disclosure to personality, adjustment and self-actualization. This study tested the hypothesis that a high level of self-disclosure is indicative of psychological adjustment and self-actualization. The self-disclosure scale, Social Avoidance and Distress, Fear of Negative Evaluation, Alienation and Repression-Sensitization scales were administered to 60 Ss. The results for all scales indicated that a high level of disclosure was associated with adjustment as measured by these scales. A second group of 94 Ss completed the self-disclosure scale and the Personal Orientation Inventory, a measure of self-actualization. The results indicated that high disclosers compared to low disclosers had achieved a higher level of self-actualization. That is, there were significant correlations between self-disclosure and measures of self-actualization such as Time Competence, Internal Support, Existentiality, Self-Acceptance and Capacity for Intimate Contact.", "PMID": 977737}
{"id": "pubmed23n0032_11311", "title": "The influence of interviewer self-disclosure and verbal reinforcement on personality tests.", "content": "The effects of prior interview experiences on subsequent test responding were examined by comparing the influence of three interviewer behaviors: interviewer self-disclosure, interviewer verbal reinforcement of self-referent statements, and direct instructions to be self-disclosive. Performance was contrasted with a control interview condition characterized by no interviewer self-disclosure or self-referent reinforcement and with a no-interview condition. Responses on measures of self-disclosure and anxiety indicated that female Ss tended to be more self-disclosive than males in general, but were relatively uninfluenced by experimental condition. For male Ss, verbal reinforcement increased self-disclosure relative to the interviewer seld-disclosure condition. Interviewer self-disclosure was shown to result in more favorable perceptions of the self and the interviewer, particularly for males, and greater social evaluative anxiety for females. Both these factors are discussed as possibly counteracting the modelling effects of interviewer self-disclosure. An interpretation of the results in terms of interactions between S sex, interviewer sex, and experimental condition is proposed and implications for related research discussed.", "contents": "The influence of interviewer self-disclosure and verbal reinforcement on personality tests. The effects of prior interview experiences on subsequent test responding were examined by comparing the influence of three interviewer behaviors: interviewer self-disclosure, interviewer verbal reinforcement of self-referent statements, and direct instructions to be self-disclosive. Performance was contrasted with a control interview condition characterized by no interviewer self-disclosure or self-referent reinforcement and with a no-interview condition. Responses on measures of self-disclosure and anxiety indicated that female Ss tended to be more self-disclosive than males in general, but were relatively uninfluenced by experimental condition. For male Ss, verbal reinforcement increased self-disclosure relative to the interviewer seld-disclosure condition. Interviewer self-disclosure was shown to result in more favorable perceptions of the self and the interviewer, particularly for males, and greater social evaluative anxiety for females. Both these factors are discussed as possibly counteracting the modelling effects of interviewer self-disclosure. An interpretation of the results in terms of interactions between S sex, interviewer sex, and experimental condition is proposed and implications for related research discussed.", "PMID": 977738}
{"id": "pubmed23n0032_11312", "title": "Normalizing the MMPI.", "content": "Effective psychological services require recognition of a client's strengths as well as weaknesses. Personality tests such as the MMPI, however, focus attention upon the abnormal and deviant attributes of personality. Therefore, a psychologist should have an understanding of various adaptive behaviors that may be indicated by moderately elevated MMPI scale scores. Because most of the literature deals with the pathological ramifications of scale elevations, the authors developed a conceptual perspective of counterpart descriptors for each MMPI clinical scale. These descriptors are presented so that apparently contradictory sets of behaviors for elevated scores on any given scale can be viewed on a continuum from adaptive to maladaptive behavior. An armamentarium of MMPI personality traits should include positive as well as negative considerations to provide a fully comprehensive basis for the formulation of realistic assessments of human potentialities.", "contents": "Normalizing the MMPI. Effective psychological services require recognition of a client's strengths as well as weaknesses. Personality tests such as the MMPI, however, focus attention upon the abnormal and deviant attributes of personality. Therefore, a psychologist should have an understanding of various adaptive behaviors that may be indicated by moderately elevated MMPI scale scores. Because most of the literature deals with the pathological ramifications of scale elevations, the authors developed a conceptual perspective of counterpart descriptors for each MMPI clinical scale. These descriptors are presented so that apparently contradictory sets of behaviors for elevated scores on any given scale can be viewed on a continuum from adaptive to maladaptive behavior. An armamentarium of MMPI personality traits should include positive as well as negative considerations to provide a fully comprehensive basis for the formulation of realistic assessments of human potentialities.", "PMID": 977739}
{"id": "pubmed23n0032_11313", "title": "Relationships between Spielberger trait anxiety and Lykken social and physical trait anxiety.", "content": "To determine the relationship between Spielberger's measure of trait anxiety and social-interpersonal vs. physical danger trait anxiety, Ss were administered and trait scale of the State-Trait Anxiety Inventory (STAI) and Lykken's Activity Preference Questionnaire (APQ). Results of correlative analyses indicated that the STAI-Trait measure correlated significantly with APQ-Social Anxiety, but not with APQ-Physical Anxiety. Results were discussed in terms of the implications for predicting state anxiety in different situations by the use of unidimensional vs. multidimensional trait anxiety measures.", "contents": "Relationships between Spielberger trait anxiety and Lykken social and physical trait anxiety. To determine the relationship between Spielberger's measure of trait anxiety and social-interpersonal vs. physical danger trait anxiety, Ss were administered and trait scale of the State-Trait Anxiety Inventory (STAI) and Lykken's Activity Preference Questionnaire (APQ). Results of correlative analyses indicated that the STAI-Trait measure correlated significantly with APQ-Social Anxiety, but not with APQ-Physical Anxiety. Results were discussed in terms of the implications for predicting state anxiety in different situations by the use of unidimensional vs. multidimensional trait anxiety measures.", "PMID": 977740}
{"id": "pubmed23n0032_11314", "title": "Personality patterns among correctional officer applicants.", "content": "The MMPI profiles of 359 correctional officer applicant were cluster analyzed, which resulted in the identification of five relatively homogeneous subgroups. While MMPI performance was not related to later events in the correctional careers of the Ss, certain similarities were noted between the officer group and two samples of inmates previously studied in a comparable fashion. In this respect, although the officers manifested generally lower profile elevations than inmates, configural similarities were noted between the average inmate and the officer profiles, and partial overlap was seen between the profile types identified in the two groups. The implications of the findings for occupationally adaptive and maladaptive correctional officer behavior were discussed.", "contents": "Personality patterns among correctional officer applicants. The MMPI profiles of 359 correctional officer applicant were cluster analyzed, which resulted in the identification of five relatively homogeneous subgroups. While MMPI performance was not related to later events in the correctional careers of the Ss, certain similarities were noted between the officer group and two samples of inmates previously studied in a comparable fashion. In this respect, although the officers manifested generally lower profile elevations than inmates, configural similarities were noted between the average inmate and the officer profiles, and partial overlap was seen between the profile types identified in the two groups. The implications of the findings for occupationally adaptive and maladaptive correctional officer behavior were discussed.", "PMID": 977741}
{"id": "pubmed23n0032_11315", "title": "Correspondence of the MMPI and Mini-Mult among female reformatory inmates.", "content": "Comparison of the MMPI and Mini-Mult scales yielded significant scale-by-scale correlations. However, the group means were different in 6 of the 11 possible scales, and there was a general tendency for the Mini-Mult to over-estimate MMPI scales. The Mini-Mult proved to be highly ineffective to detect MMPI invalid profiles. In addition, agreement on profile configurations or high points and degree of pathology as indicated by number of significant clinical scale elevations was very poor. The results of this investigation are similar to the findings of Rybolt and Lambert (1975), as well as those of Platt and Scura (1972), and lead one to recommend that the Mini-Mult not be employed as a clonical assessment instrument with incarcerated adult female populations. Its multiple deficits make clinical use hazardous.", "contents": "Correspondence of the MMPI and Mini-Mult among female reformatory inmates. Comparison of the MMPI and Mini-Mult scales yielded significant scale-by-scale correlations. However, the group means were different in 6 of the 11 possible scales, and there was a general tendency for the Mini-Mult to over-estimate MMPI scales. The Mini-Mult proved to be highly ineffective to detect MMPI invalid profiles. In addition, agreement on profile configurations or high points and degree of pathology as indicated by number of significant clinical scale elevations was very poor. The results of this investigation are similar to the findings of Rybolt and Lambert (1975), as well as those of Platt and Scura (1972), and lead one to recommend that the Mini-Mult not be employed as a clonical assessment instrument with incarcerated adult female populations. Its multiple deficits make clinical use hazardous.", "PMID": 977742}
{"id": "pubmed23n0032_11316", "title": "Relationship of internal-external control and United States suicide rates, 1966-1973.", "content": "The annual variations in scores obtained on the Rotter Internal-External (I-E) control scale by United States college students between the years 1966 and 1973 were related to the concomitant annual variations in suicide rates for the total United States population and for eight separate age groups. The I-E scores increased substantially during this time period and were correlated positively with the concomitantly increasing suicide rates among relatively young persons (in and below the 35-44 year age group). However, the suicide rates among older persons generally decreased during this time period and thus were correlated negatively with the I-E scores. These results suggest that perception of internal-external control did not change among older persons as they did among younger persons, perhaps because older persons might be less susceptible to the cultural influences that affect the perceived control of younger persons. Results of this and earlier research suggest that cultural characteristics that foster high perceptions of external control also foster suicidal behavior and suggest the value of attempts to convey perceptions of internal control to psychotherapy clients.", "contents": "Relationship of internal-external control and United States suicide rates, 1966-1973. The annual variations in scores obtained on the Rotter Internal-External (I-E) control scale by United States college students between the years 1966 and 1973 were related to the concomitant annual variations in suicide rates for the total United States population and for eight separate age groups. The I-E scores increased substantially during this time period and were correlated positively with the concomitantly increasing suicide rates among relatively young persons (in and below the 35-44 year age group). However, the suicide rates among older persons generally decreased during this time period and thus were correlated negatively with the I-E scores. These results suggest that perception of internal-external control did not change among older persons as they did among younger persons, perhaps because older persons might be less susceptible to the cultural influences that affect the perceived control of younger persons. Results of this and earlier research suggest that cultural characteristics that foster high perceptions of external control also foster suicidal behavior and suggest the value of attempts to convey perceptions of internal control to psychotherapy clients.", "PMID": 977743}
{"id": "pubmed23n0032_11317", "title": "The effects of videotape models on the behavior of chronic schizophrenics.", "content": "The effects of \"good\" and \"poor\" peer models on the task performance of 45 chronic schizophrenic males were investigated. The findings revealed that patients who viewed a good peer model did significantly better on a subsequent task than patients who viewed either a poor model or no model. The use of peer models in the treatment of chronic schizophrenics was suggested.", "contents": "The effects of videotape models on the behavior of chronic schizophrenics. The effects of \"good\" and \"poor\" peer models on the task performance of 45 chronic schizophrenic males were investigated. The findings revealed that patients who viewed a good peer model did significantly better on a subsequent task than patients who viewed either a poor model or no model. The use of peer models in the treatment of chronic schizophrenics was suggested.", "PMID": 977744}
{"id": "pubmed23n0032_11318", "title": "Using the MMPI 168 with medical inpatients.", "content": "The potential utility of the MMPI 168 with two inpatient medical populations was explored in the present study. Correlations and clinically relevant comparisons suggested that the MMPI 168 predicted the standard MMPI with a high degree of accuracy.", "contents": "Using the MMPI 168 with medical inpatients. The potential utility of the MMPI 168 with two inpatient medical populations was explored in the present study. Correlations and clinically relevant comparisons suggested that the MMPI 168 predicted the standard MMPI with a high degree of accuracy.", "PMID": 977745}
{"id": "pubmed23n0032_11319", "title": "Personality characteristics of high and low aggressive adolescents in residential treatment.", "content": "Variables related to aggression were explored in a population of psychiatrically disturbed male adolescents in a residential treatment setting. The Marlowe-Crowne Social Desirability Scale, Daydreaming Questionaire, Byrne Repression-Sensitization Scale, and the Buss-Durkee Hostility Inventory were administered to high and low aggressive patients. Multivariate analyses of the data indicated that high aggressors were significantly lower in their social desirability needs and significantly higher in their daydreaming, hostile, attitudes and sensitization than were low aggressive patients. These findings were discussed relative to a greater understanding of aggression in emotionally disturbed adolescents and implications for treatment.", "contents": "Personality characteristics of high and low aggressive adolescents in residential treatment. Variables related to aggression were explored in a population of psychiatrically disturbed male adolescents in a residential treatment setting. The Marlowe-Crowne Social Desirability Scale, Daydreaming Questionaire, Byrne Repression-Sensitization Scale, and the Buss-Durkee Hostility Inventory were administered to high and low aggressive patients. Multivariate analyses of the data indicated that high aggressors were significantly lower in their social desirability needs and significantly higher in their daydreaming, hostile, attitudes and sensitization than were low aggressive patients. These findings were discussed relative to a greater understanding of aggression in emotionally disturbed adolescents and implications for treatment.", "PMID": 977746}
{"id": "pubmed23n0032_11320", "title": "The identification of brain damage by an abbreviated form of the Halstead-Reitan Neuropsychological Battery.", "content": "In recent years there has been an increased interest in the development of neuropsychological tests that can diagnose accurately brain damage. The most successful of these test are represented in the Halstead-Reitan Neuropsychological Battery, which has been shown to be 90% or more effective in the diagnosis of brain damage. However, this battery is not available to many psychologists or clients because of its length, cost, and the considerable background required to use it properly. The present study tested 121 Ss (30 normal, 91 brain-damaged) to develop an abbreviated version of the battery that is relatively brief (1 hour) and inexpensive, but accurate. It was found by discriminant analysis that the abbreviated battery is capable of diagnosing brain damage equally as well as the full battery 93% of the time. The limitations and advantages of the abbreviated battery are discussed.", "contents": "The identification of brain damage by an abbreviated form of the Halstead-Reitan Neuropsychological Battery. In recent years there has been an increased interest in the development of neuropsychological tests that can diagnose accurately brain damage. The most successful of these test are represented in the Halstead-Reitan Neuropsychological Battery, which has been shown to be 90% or more effective in the diagnosis of brain damage. However, this battery is not available to many psychologists or clients because of its length, cost, and the considerable background required to use it properly. The present study tested 121 Ss (30 normal, 91 brain-damaged) to develop an abbreviated version of the battery that is relatively brief (1 hour) and inexpensive, but accurate. It was found by discriminant analysis that the abbreviated battery is capable of diagnosing brain damage equally as well as the full battery 93% of the time. The limitations and advantages of the abbreviated battery are discussed.", "PMID": 977747}
{"id": "pubmed23n0032_11321", "title": "The effects of verbal and nonverbal contingent reinforcement upon the intelligence test performance of black adults.", "content": "This study examined the effects of contingent verbal and nonverbal reinforcement upon the intelligence test performance of bladk adults. Thirty-six Ss (half male and half female) were pretested on the Quick Test and then administered the complete WAIS under one of three treatment conditions: verbal reinforcement, nonverbal reinforcement or no reinforcement (control condition). Verbal reinforcement was administered as statements of \"good\" or \"fine\" by the E, while nonverbal reinforcement took the form of a nod of the head or a statement of \"Mmhm.\" The primary analysis of the data indicated no significant differences between the treatment conditions.", "contents": "The effects of verbal and nonverbal contingent reinforcement upon the intelligence test performance of black adults. This study examined the effects of contingent verbal and nonverbal reinforcement upon the intelligence test performance of bladk adults. Thirty-six Ss (half male and half female) were pretested on the Quick Test and then administered the complete WAIS under one of three treatment conditions: verbal reinforcement, nonverbal reinforcement or no reinforcement (control condition). Verbal reinforcement was administered as statements of \"good\" or \"fine\" by the E, while nonverbal reinforcement took the form of a nod of the head or a statement of \"Mmhm.\" The primary analysis of the data indicated no significant differences between the treatment conditions.", "PMID": 977748}
{"id": "pubmed23n0032_11322", "title": "Bender Gestalt recall: memory measure or intelligence estimate.", "content": "WAIS subtest standard scores, IQ scores, and factorial deviation quotients were correlated with Bender Gestalt recall scores for 111 vocational rehabilitation clients. The number of Bender designs recalled was associated significantly with intellectual measures, with the strength of association somewhat stronger with nonverbal than with verbal scores. Bender recall scores were correlated more highly with Perceptual Organization DQs than with Memory-Freedom from Distractibility DQs, but that difference only approached significance. Despite the relationships between Bender recall scores and intelligence scores, the Bender recall task could not classify Ss as to general intelligence level with greater accuracy than could be obtained with the WAIS Vocabulary subtest alone.", "contents": "Bender Gestalt recall: memory measure or intelligence estimate. WAIS subtest standard scores, IQ scores, and factorial deviation quotients were correlated with Bender Gestalt recall scores for 111 vocational rehabilitation clients. The number of Bender designs recalled was associated significantly with intellectual measures, with the strength of association somewhat stronger with nonverbal than with verbal scores. Bender recall scores were correlated more highly with Perceptual Organization DQs than with Memory-Freedom from Distractibility DQs, but that difference only approached significance. Despite the relationships between Bender recall scores and intelligence scores, the Bender recall task could not classify Ss as to general intelligence level with greater accuracy than could be obtained with the WAIS Vocabulary subtest alone.", "PMID": 977749}
{"id": "pubmed23n0032_11323", "title": "Curative factors in psychotherapeutic and growth groups.", "content": "A 60-item Q-sort that represented 12 \"curative factors\" regarded as crucial to the efficacy of psychotherapeutic groups by Yalom (1970) was administered to volunteer undergraduate participants after the end of 20-hour (2 hours weekly) personal growth groups. The Q-sort items were ranked very similarly for Helpfulness in the group experience by the growth groupers and by Yalom's successful group psychotherapy outpatients. Members of both kinds of groups linked Helpfulness strongly with Yalom's Interpersonal Input and Catharsis measures. The content of these variables' salient items closely resembled two broader dimensions of interpersonal behavior, Acceptance vs. Rejection of Others and Self-Acceptance vs. Rejection, which appear prepotent (Hurley, 1976) in a wide variety of group settings.", "contents": "Curative factors in psychotherapeutic and growth groups. A 60-item Q-sort that represented 12 \"curative factors\" regarded as crucial to the efficacy of psychotherapeutic groups by Yalom (1970) was administered to volunteer undergraduate participants after the end of 20-hour (2 hours weekly) personal growth groups. The Q-sort items were ranked very similarly for Helpfulness in the group experience by the growth groupers and by Yalom's successful group psychotherapy outpatients. Members of both kinds of groups linked Helpfulness strongly with Yalom's Interpersonal Input and Catharsis measures. The content of these variables' salient items closely resembled two broader dimensions of interpersonal behavior, Acceptance vs. Rejection of Others and Self-Acceptance vs. Rejection, which appear prepotent (Hurley, 1976) in a wide variety of group settings.", "PMID": 977750}
{"id": "pubmed23n0032_11324", "title": "Comparison of verbal reinforcement with information feedback in analogue psychotherapy.", "content": "Thirty Ss were assigned randomly to one of the two experimental analogue psychotherapy groups and a control group. In the experimental psychotherapy groups two different psychotherapy techniques were used for eight sessions. The two psychotherapy techniques employed were one-word verbal reinforcement (VR) and lengthy verbal information feedback (IF). Three therapists each demonstrated significant differences (P less than .001) in their verbal interactions with the Ss when they used the two different psychotherapy techniques. The results indicated that both treatment techniques were significantly effective (p less than .05) in improving self-ratings of self-concept. However, the two psychotherapy techniques were differentially effective in improving social skills as measured by behavioral ratings.", "contents": "Comparison of verbal reinforcement with information feedback in analogue psychotherapy. Thirty Ss were assigned randomly to one of the two experimental analogue psychotherapy groups and a control group. In the experimental psychotherapy groups two different psychotherapy techniques were used for eight sessions. The two psychotherapy techniques employed were one-word verbal reinforcement (VR) and lengthy verbal information feedback (IF). Three therapists each demonstrated significant differences (P less than .001) in their verbal interactions with the Ss when they used the two different psychotherapy techniques. The results indicated that both treatment techniques were significantly effective (p less than .05) in improving self-ratings of self-concept. However, the two psychotherapy techniques were differentially effective in improving social skills as measured by behavioral ratings.", "PMID": 977751}
{"id": "pubmed23n0032_11325", "title": "Suicidal age and childhood onychophagia among psychotic veterans.", "content": "The relationship between childhood onychophagia (fingernail biting) and the suicidal and nonsuicidal mean age among 150 hospitalized psychotic veterans was investigated. The results show that the suicidal mean age is significantly younger than the nonsuicidal mean age and that the childhood onychophagia variable \"focal suicide\" significantly lowers the mean age of suicidal veterans, but does not significantly lower the mean age of nonsuicidal veterans.", "contents": "Suicidal age and childhood onychophagia among psychotic veterans. The relationship between childhood onychophagia (fingernail biting) and the suicidal and nonsuicidal mean age among 150 hospitalized psychotic veterans was investigated. The results show that the suicidal mean age is significantly younger than the nonsuicidal mean age and that the childhood onychophagia variable \"focal suicide\" significantly lowers the mean age of suicidal veterans, but does not significantly lower the mean age of nonsuicidal veterans.", "PMID": 977752}
{"id": "pubmed23n0032_11326", "title": "The effect of relocation on the satisfaction of psychiatric inpatients.", "content": "Fifty-one psychiatric inpatients were tested on a 36-item satisfaction scale before and after a reorganization at Harlem Valley Psychiatric Center. Patients were divided into three groups (Low, Medium, and High Stress) on the basis of whether they experienced no change as a result of the reorganization, environmental change only (different building), or environmental and social change (different building, different staff, different fellow patients). Patients in the three groups were matched on the basis of chronological age, length of hospitalization, and sex. Results indicated that there were no reliable differences among the satisfaction scores of the groups as a result of the relocation. This finding supports the hypothesis that the involuntary relocation of chronic psychiatric patients to another institutional treatment environment results in few demonstrable effects.", "contents": "The effect of relocation on the satisfaction of psychiatric inpatients. Fifty-one psychiatric inpatients were tested on a 36-item satisfaction scale before and after a reorganization at Harlem Valley Psychiatric Center. Patients were divided into three groups (Low, Medium, and High Stress) on the basis of whether they experienced no change as a result of the reorganization, environmental change only (different building), or environmental and social change (different building, different staff, different fellow patients). Patients in the three groups were matched on the basis of chronological age, length of hospitalization, and sex. Results indicated that there were no reliable differences among the satisfaction scores of the groups as a result of the relocation. This finding supports the hypothesis that the involuntary relocation of chronic psychiatric patients to another institutional treatment environment results in few demonstrable effects.", "PMID": 977753}
{"id": "pubmed23n0032_11327", "title": "The effect of structured and unstructured leader roles on internal and external group participants.", "content": "This study assessed the impact of structured and unstructured leader roles on measures of outcome for Ss who differed on the pretest dimension \"locus of control.\" On the basis of their pretest locus of control scores, Ss were assigned to a structured or an unstructured marathon group. The treatment in the two 16-hour -arathons consisted of a defined series of exercises; the only difference between the two groups was the degree of leader control over member participation. As predicted, internal Ss in the unstructured group rated the leader and the group more psoitively than did external Ss, while the reverse responsivity occured in the structured group. Significant Locus of Control X Treatment interactions indicated that internal and external Ss reflected differential shifts in general anxiety, general depression, and locus of control as a function of treatment. The relationship between changes in self-actualization and locus of control and changes in conflict-handling styles and negative affects differed as a function of locus of control and treatment condition. The overall results coupled with prior findings tentatively support the appropriateness of an unstructured leader role for internal scores and a structured leader role for external scorers.", "contents": "The effect of structured and unstructured leader roles on internal and external group participants. This study assessed the impact of structured and unstructured leader roles on measures of outcome for Ss who differed on the pretest dimension \"locus of control.\" On the basis of their pretest locus of control scores, Ss were assigned to a structured or an unstructured marathon group. The treatment in the two 16-hour -arathons consisted of a defined series of exercises; the only difference between the two groups was the degree of leader control over member participation. As predicted, internal Ss in the unstructured group rated the leader and the group more psoitively than did external Ss, while the reverse responsivity occured in the structured group. Significant Locus of Control X Treatment interactions indicated that internal and external Ss reflected differential shifts in general anxiety, general depression, and locus of control as a function of treatment. The relationship between changes in self-actualization and locus of control and changes in conflict-handling styles and negative affects differed as a function of locus of control and treatment condition. The overall results coupled with prior findings tentatively support the appropriateness of an unstructured leader role for internal scores and a structured leader role for external scorers.", "PMID": 977754}
{"id": "pubmed23n0032_11328", "title": "The impact of differential shifts in leader structure on the outcome of internal and external group participants.", "content": "This study investigated the interaction between locus of control and high and low degrees of structure of leader role as related to outcome. On the basis of their pretreatment locus of control score, college students were assigned randomly to one of five groups: a group that began unstructured and shifted to a structured format at the midpoint of the group, a group that began structured and shifted to an unstructured format at the midpoint, a group that was structured throughout, group that was unstructured throughout, and a no-treatment control group. Treatment in the 23-hour marathon groups consisted of the same sequence of planned group exercises; the leader varied his structure in accordance with the experimental design. The results indicated that an initial highly structured leader role followed by a relatively unstructured leader role presented at the midpoint of treatment fostered the greatest gains in self-actualization in both internal and external Ss.", "contents": "The impact of differential shifts in leader structure on the outcome of internal and external group participants. This study investigated the interaction between locus of control and high and low degrees of structure of leader role as related to outcome. On the basis of their pretreatment locus of control score, college students were assigned randomly to one of five groups: a group that began unstructured and shifted to a structured format at the midpoint of the group, a group that began structured and shifted to an unstructured format at the midpoint, a group that was structured throughout, group that was unstructured throughout, and a no-treatment control group. Treatment in the 23-hour marathon groups consisted of the same sequence of planned group exercises; the leader varied his structure in accordance with the experimental design. The results indicated that an initial highly structured leader role followed by a relatively unstructured leader role presented at the midpoint of treatment fostered the greatest gains in self-actualization in both internal and external Ss.", "PMID": 977755}
{"id": "pubmed23n0032_11329", "title": "Affection for patients as a factor in therapists' outcome judgements.", "content": "This study was concerned with psychotherapists' evaluations of the outcome of therapy. Staff and resident psychiatrists employed in 23-item questionnaire to rate the seccess of psychotherapy with 85 of their inpatients. These were patients for whom psychotherapy constituted a significant part of the treatment that they received in the hospital. The therapists' responses to the questionnaire items were intercorrelated. The results indicated that a major aspect of a psychotherapist's judgment of the success of treatment-as these judgments usually are employed in current psychotherapy research-is the therapist's affective reaction to the patient, Better-liked patients were viewed as having improved more. However, when the therapists' responses were subjected to factor analysis, independent Improvement and Affection factors emerged. It is suggested that when therapists' ratings of success are used in psychotherapy research they should be treated or refined statistically to minimize potential confounding with their affection for patients.", "contents": "Affection for patients as a factor in therapists' outcome judgements. This study was concerned with psychotherapists' evaluations of the outcome of therapy. Staff and resident psychiatrists employed in 23-item questionnaire to rate the seccess of psychotherapy with 85 of their inpatients. These were patients for whom psychotherapy constituted a significant part of the treatment that they received in the hospital. The therapists' responses to the questionnaire items were intercorrelated. The results indicated that a major aspect of a psychotherapist's judgment of the success of treatment-as these judgments usually are employed in current psychotherapy research-is the therapist's affective reaction to the patient, Better-liked patients were viewed as having improved more. However, when the therapists' responses were subjected to factor analysis, independent Improvement and Affection factors emerged. It is suggested that when therapists' ratings of success are used in psychotherapy research they should be treated or refined statistically to minimize potential confounding with their affection for patients.", "PMID": 977756}
{"id": "pubmed23n0032_11330", "title": "The validity of the Rorschach Prognostic Rating Scale with incarcerated offenders.", "content": "Previous research has shown that the Rorschach Prognostic Rating Scale reliably predicts the adjustment capacity of various S types to a variety of situations. The present study investigated the efficacy of the RPRS to suggest the adjustment capacity of prisoners to various aspects of their institutional environment. Twenty-five male inmates of the Petersburg Federal Reformatory served as Ss. Group therapy, dormitory behavior, work progress adjustment ratings, and RPRS scores were obtained for each S. Intercorrelations among the adjustment measures and the RPRS total and component scores revealed no significant correlations between the adjustment measures and the RPRS total score. However, in employing a moximum R stepwise multiple correlation procedure with the RPRS compontnts, significant multiple Rs resulted for two of the three criterion measures. These results were discussed in terms of the earlier research with the RPRS, and suggestions for future research were made.", "contents": "The validity of the Rorschach Prognostic Rating Scale with incarcerated offenders. Previous research has shown that the Rorschach Prognostic Rating Scale reliably predicts the adjustment capacity of various S types to a variety of situations. The present study investigated the efficacy of the RPRS to suggest the adjustment capacity of prisoners to various aspects of their institutional environment. Twenty-five male inmates of the Petersburg Federal Reformatory served as Ss. Group therapy, dormitory behavior, work progress adjustment ratings, and RPRS scores were obtained for each S. Intercorrelations among the adjustment measures and the RPRS total and component scores revealed no significant correlations between the adjustment measures and the RPRS total score. However, in employing a moximum R stepwise multiple correlation procedure with the RPRS compontnts, significant multiple Rs resulted for two of the three criterion measures. These results were discussed in terms of the earlier research with the RPRS, and suggestions for future research were made.", "PMID": 977757}
{"id": "pubmed23n0032_11331", "title": "Sex guilt and preferences for illegal drugs among drug abusers.", "content": "This study investigated the relationship between sex guilt and drug preferences. Seventy polydrug users completed a drug preference inventory and the Mosher Forced-Choice Sex Guilt Subscale. Results indicated that individuals who prefer sedatives are higher in sex guilt than either individuals who prefer stimulants or individuals with no definite preference.", "contents": "Sex guilt and preferences for illegal drugs among drug abusers. This study investigated the relationship between sex guilt and drug preferences. Seventy polydrug users completed a drug preference inventory and the Mosher Forced-Choice Sex Guilt Subscale. Results indicated that individuals who prefer sedatives are higher in sex guilt than either individuals who prefer stimulants or individuals with no definite preference.", "PMID": 977758}
{"id": "pubmed23n0032_11332", "title": "Evidence that suggests a negative association between cigarette smoking and learning performance.", "content": "The results of this study indicate that tobacco smoking may have a deleterious effect on the learning process. One hundred and fifteen male volunteers were assessed on four learning tasks. Those Ss who smoked in excess of 12 cigarettes per day did significantly less well, as a group, than nonsmokers and light smokers on three of the four learning tests.", "contents": "Evidence that suggests a negative association between cigarette smoking and learning performance. The results of this study indicate that tobacco smoking may have a deleterious effect on the learning process. One hundred and fifteen male volunteers were assessed on four learning tasks. Those Ss who smoked in excess of 12 cigarettes per day did significantly less well, as a group, than nonsmokers and light smokers on three of the four learning tests.", "PMID": 977759}
{"id": "pubmed23n0032_11333", "title": "Relationship between psychopathology, experienced control and perceived locus of control: in search of alcoholic subtypes.", "content": "Alcoholic Ss were categorized into four subtypes based upon differential levels of perceived locus of control and experienced control. The relationship between generalized psychopathology, as measured by the MMPI, and subtype classification was investigated. Alcoholics with an internal locus of control and high levels of experienced control exhibited the least psychopathology. The greatest degree of psychopathology was found among Ss with an external locus of control and minimal levels of experienced control. The implications of these results with respect to psychosocial functioning and drinking behavior among alcoholic subgroups were discussed.", "contents": "Relationship between psychopathology, experienced control and perceived locus of control: in search of alcoholic subtypes. Alcoholic Ss were categorized into four subtypes based upon differential levels of perceived locus of control and experienced control. The relationship between generalized psychopathology, as measured by the MMPI, and subtype classification was investigated. Alcoholics with an internal locus of control and high levels of experienced control exhibited the least psychopathology. The greatest degree of psychopathology was found among Ss with an external locus of control and minimal levels of experienced control. The implications of these results with respect to psychosocial functioning and drinking behavior among alcoholic subgroups were discussed.", "PMID": 977760}
{"id": "pubmed23n0032_11334", "title": "Serial liver biopsies in blood donors with persistent HBs antigenaemia.", "content": "Initial liver biopsies from asymptomatic HBs antigen positive blood donors showed a range of histological abnormalities ranging from minor parenchymal lesions to cirrhosis. Twenty of these have now been followed up for periods of up to four years and during that time have had at least two liver biopsies. Throughout the period of study all the donors have remained carriers of HBs Ag, and there was no significant variation in the titers of antigen or the electron microscopic appearances of the serum in individual donors. The histological appearances of subsequent liver biopsies were not always the same as those seen initially, and while there appeared to be some improvement in three cases, there were nine in which the histological appearances were worse.", "contents": "Serial liver biopsies in blood donors with persistent HBs antigenaemia. Initial liver biopsies from asymptomatic HBs antigen positive blood donors showed a range of histological abnormalities ranging from minor parenchymal lesions to cirrhosis. Twenty of these have now been followed up for periods of up to four years and during that time have had at least two liver biopsies. Throughout the period of study all the donors have remained carriers of HBs Ag, and there was no significant variation in the titers of antigen or the electron microscopic appearances of the serum in individual donors. The histological appearances of subsequent liver biopsies were not always the same as those seen initially, and while there appeared to be some improvement in three cases, there were nine in which the histological appearances were worse.", "PMID": 977761}
{"id": "pubmed23n0032_11335", "title": "Immunoglobulins and complement in pleural effusions associated with bronchogenic carcinoma.", "content": "Levels of IgG, IgA, IgM, the total haemolytic complement (CH50), and the individual components C1q, C3, C4, C6, and C7 were measured in 29 pleural effusions. Of these, 18 were associated with carcinoma of the bronchus and 11 were non-malignant effusions including empyemas. The level of IgG was significantly lower in the malignant group when compared with non-malignant effusions. The usefulness of measurements of IgG with respect to malignant effusions associated with carcinoma of the bronchus requires an expanded study to show whether it has any real diagnostic value. There were no significant differences in other immunoglobulins, the CH50, and individual complement components between the two groups. The identification of total haemolytic activity in the majority of effusions in both groups indicates that all nine components of the classical pathway of complement, including macromolecules such as C1, can be present in pleural fluids.", "contents": "Immunoglobulins and complement in pleural effusions associated with bronchogenic carcinoma. Levels of IgG, IgA, IgM, the total haemolytic complement (CH50), and the individual components C1q, C3, C4, C6, and C7 were measured in 29 pleural effusions. Of these, 18 were associated with carcinoma of the bronchus and 11 were non-malignant effusions including empyemas. The level of IgG was significantly lower in the malignant group when compared with non-malignant effusions. The usefulness of measurements of IgG with respect to malignant effusions associated with carcinoma of the bronchus requires an expanded study to show whether it has any real diagnostic value. There were no significant differences in other immunoglobulins, the CH50, and individual complement components between the two groups. The identification of total haemolytic activity in the majority of effusions in both groups indicates that all nine components of the classical pathway of complement, including macromolecules such as C1, can be present in pleural fluids.", "PMID": 977762}
{"id": "pubmed23n0032_11336", "title": "Hyperfibrinogenaemia and hyperviscosity in sickle-cell crisis.", "content": "Plasma fibrinogen concentration and whole-blood viscosity, the latter measured at two shear rates (23 and 230 sec-1), were estimated during eight episodes of sickle-cell crisis and compared with values in 26 sickle-cell anaemia patients who were not in crisis. Painful crisis was associated with a significant increase in both plasma fibrinogen and whole-blood viscosity. Increased fibrinogen-erythrocyte interaction in vivo may be a significant contributory factor to raising blood viscosity and precipitating vaso-occlusive crisis in sickle-cell disease.", "contents": "Hyperfibrinogenaemia and hyperviscosity in sickle-cell crisis. Plasma fibrinogen concentration and whole-blood viscosity, the latter measured at two shear rates (23 and 230 sec-1), were estimated during eight episodes of sickle-cell crisis and compared with values in 26 sickle-cell anaemia patients who were not in crisis. Painful crisis was associated with a significant increase in both plasma fibrinogen and whole-blood viscosity. Increased fibrinogen-erythrocyte interaction in vivo may be a significant contributory factor to raising blood viscosity and precipitating vaso-occlusive crisis in sickle-cell disease.", "PMID": 977763}
{"id": "pubmed23n0032_11337", "title": "Erroneous values for the total white cell count and ESR in patients with cryoglobulinaemia.", "content": "Temperature dependent fluctuations in the erythrocyte sedimentation rate were noted during the assessment of one patient with symptomatic cryoglobulinaemia. Two patients with this condition were found to have erroneously high leucocyte counts when estimated by the Model S Coulter Counter. The artefact was directly related to the degree of exposure to cold before the estimation was performed and appeared to be due to the formation of microaggregates of cryoglobulin and fibrinogen.", "contents": "Erroneous values for the total white cell count and ESR in patients with cryoglobulinaemia. Temperature dependent fluctuations in the erythrocyte sedimentation rate were noted during the assessment of one patient with symptomatic cryoglobulinaemia. Two patients with this condition were found to have erroneously high leucocyte counts when estimated by the Model S Coulter Counter. The artefact was directly related to the degree of exposure to cold before the estimation was performed and appeared to be due to the formation of microaggregates of cryoglobulin and fibrinogen.", "PMID": 977764}
{"id": "pubmed23n0032_11338", "title": "Changing blood picture in sickle-cell anaemia from shortly after birth to adolescence.", "content": "The blood picture of children with sickle-cell anaemia was found to change with age. The changes were most marked in the first year but the mean level of haemoglobin, haemoglobin F, and target cells fell until adolescence, and irreversibly sickled cells rose. Reticulocytes, Howell Jolly bodies, and normablasts altered little after one year. The fall in haemoglobin F suggested a delayed changeover from fetal to adult haemoglobin production. It was concluded that the blood changes in sickle-cell anaemia were progressive throughout childhood.", "contents": "Changing blood picture in sickle-cell anaemia from shortly after birth to adolescence. The blood picture of children with sickle-cell anaemia was found to change with age. The changes were most marked in the first year but the mean level of haemoglobin, haemoglobin F, and target cells fell until adolescence, and irreversibly sickled cells rose. Reticulocytes, Howell Jolly bodies, and normablasts altered little after one year. The fall in haemoglobin F suggested a delayed changeover from fetal to adult haemoglobin production. It was concluded that the blood changes in sickle-cell anaemia were progressive throughout childhood.", "PMID": 977765}
{"id": "pubmed23n0032_11339", "title": "Radioimmunoassay kits for thyroid hormones.", "content": "Characteristics of the T4 and T3 radioimmunoassay kits that are commercially available in the UK are described. Useful comparative data and criticism are given about many of these kits. Some unacceptable variation in accuracy is demonstrated.", "contents": "Radioimmunoassay kits for thyroid hormones. Characteristics of the T4 and T3 radioimmunoassay kits that are commercially available in the UK are described. Useful comparative data and criticism are given about many of these kits. Some unacceptable variation in accuracy is demonstrated.", "PMID": 977766}
{"id": "pubmed23n0032_11340", "title": "Stabilization of bromthymol blue colour in lecithin-sphingomyelin ratio determinations in amniotic fluid.", "content": "Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH of 11-3.", "contents": "Stabilization of bromthymol blue colour in lecithin-sphingomyelin ratio determinations in amniotic fluid. Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH of 11-3.", "PMID": 977767}
{"id": "pubmed23n0032_11341", "title": "A critique and case study of nodular sclerosing Hodgkin's disease.", "content": "This paper presents a critique of the present concepts of the pathology of nodular sclerosing Hodgkin's disease and particularly of the criteria required for diagnosis for the condition and its separation from other types of Hodgkin's disease. In addition, the natural history and histopathology of the condition have been studied among 104 cases of Hodgkin's disease presenting at The London Hospital, and the significance of the appearance is discussed.", "contents": "A critique and case study of nodular sclerosing Hodgkin's disease. This paper presents a critique of the present concepts of the pathology of nodular sclerosing Hodgkin's disease and particularly of the criteria required for diagnosis for the condition and its separation from other types of Hodgkin's disease. In addition, the natural history and histopathology of the condition have been studied among 104 cases of Hodgkin's disease presenting at The London Hospital, and the significance of the appearance is discussed.", "PMID": 977768}
{"id": "pubmed23n0032_11342", "title": "Cellular infiltrate of the jejunum after re-introduction of dietary gluten in children with treated coeliac disease.", "content": "Jejunal lamina propria plasma cells and eosinophils and intraepithelial lymphocytes were raised in coeliac children on gluten-containing diets, but only intraepithelial lymphocytes were increased in patients on gluten-free diets. In contrast, lamina propria lymphocytes were reduced in children with coeliad disease on gluten-containing diets but were normal in paitents on gluten-free diets. In children with coeliac disease who were studied serially, lamina propria plasma cells and eosinophils and intraepithelial lymphocytes increased, and lamina propria lymphocytes decreased, within three months of the reintroduction of gluten to the diet. These observations are essentially similar to those made in the adult form of the disease and suggest that more than one type of immunological reaction is involved in the pathogenesis of the jejunal lesion.", "contents": "Cellular infiltrate of the jejunum after re-introduction of dietary gluten in children with treated coeliac disease. Jejunal lamina propria plasma cells and eosinophils and intraepithelial lymphocytes were raised in coeliac children on gluten-containing diets, but only intraepithelial lymphocytes were increased in patients on gluten-free diets. In contrast, lamina propria lymphocytes were reduced in children with coeliad disease on gluten-containing diets but were normal in paitents on gluten-free diets. In children with coeliac disease who were studied serially, lamina propria plasma cells and eosinophils and intraepithelial lymphocytes increased, and lamina propria lymphocytes decreased, within three months of the reintroduction of gluten to the diet. These observations are essentially similar to those made in the adult form of the disease and suggest that more than one type of immunological reaction is involved in the pathogenesis of the jejunal lesion.", "PMID": 977769}
{"id": "pubmed23n0032_11343", "title": "Serum lysozyme in inflammatory bowel and coeliac disease.", "content": "Serum lysozyme levels were determined by a turbidometric method using egg white lysozyme as standard in 100 patients with Crohn's disease, 86 with ulcerative colitis, 31 with coeliac disease, and in 38 normal control subjects. Though the levels in Crohn's disease were significantly higher than those in ulcerative colitis and in coeliac disease, there was marked overlap between the disorders and control subjects, and so they were of no value in differential diagnosis. There was some evidence that serum lysozyme levels reflected disease activity in Crohn's disease but not in ulcerative colitis.", "contents": "Serum lysozyme in inflammatory bowel and coeliac disease. Serum lysozyme levels were determined by a turbidometric method using egg white lysozyme as standard in 100 patients with Crohn's disease, 86 with ulcerative colitis, 31 with coeliac disease, and in 38 normal control subjects. Though the levels in Crohn's disease were significantly higher than those in ulcerative colitis and in coeliac disease, there was marked overlap between the disorders and control subjects, and so they were of no value in differential diagnosis. There was some evidence that serum lysozyme levels reflected disease activity in Crohn's disease but not in ulcerative colitis.", "PMID": 977770}
{"id": "pubmed23n0032_11344", "title": "Morphological basis of radiological band shadows on chest radiographs.", "content": "Lungs of unselected cadavers were fixed at necropsy using a formalin vapour technique. \"Band shadows\" were identified in the excised lungs and these were correlated with in vivo radiographs and with the morphological changes in the lung. Persistent shadows were produced by pulmonary infarction, subsegmental atelectasis, and septal fibrosis singly and in combination. Potentially transient shadows were seen in association with atelectasis and pulmonary oedema.", "contents": "Morphological basis of radiological band shadows on chest radiographs. Lungs of unselected cadavers were fixed at necropsy using a formalin vapour technique. \"Band shadows\" were identified in the excised lungs and these were correlated with in vivo radiographs and with the morphological changes in the lung. Persistent shadows were produced by pulmonary infarction, subsegmental atelectasis, and septal fibrosis singly and in combination. Potentially transient shadows were seen in association with atelectasis and pulmonary oedema.", "PMID": 977771}
{"id": "pubmed23n0032_11345", "title": "An evaluation of automatic coding of surgical pathology reports.", "content": "The use of the Systematised Nomenclature of Pathology as the basis of an indexing system to histopathological data is outlined. Two computer programs which perform the task of producing codes from free English summaries are compared. It is concluded that a simple system has a great deal to offer the pathologist who is prepared to accept a set of constraints.", "contents": "An evaluation of automatic coding of surgical pathology reports. The use of the Systematised Nomenclature of Pathology as the basis of an indexing system to histopathological data is outlined. Two computer programs which perform the task of producing codes from free English summaries are compared. It is concluded that a simple system has a great deal to offer the pathologist who is prepared to accept a set of constraints.", "PMID": 977772}
{"id": "pubmed23n0032_11346", "title": "Sea-blue histiocytosis associated with hyperlipidaemia.", "content": "A patient with sea-blue histiocytosis in spleen and bone marrow with an accompanying hyperlipidaemia is described. The hyperlipidaemia was due to an increase in \"free\" cholesterol, lecithin, and triglycerides. Despite these findings lecithin-cholesterol acyl transferase activity was normal. Although the precise biochemical defect was not identified, there was a failure of transport of cholesterol from chylomicrons in vitro. We propose that the sea-blue histiocyte is a marker, in some cases, of abnormal lipid metabolism.", "contents": "Sea-blue histiocytosis associated with hyperlipidaemia. A patient with sea-blue histiocytosis in spleen and bone marrow with an accompanying hyperlipidaemia is described. The hyperlipidaemia was due to an increase in \"free\" cholesterol, lecithin, and triglycerides. Despite these findings lecithin-cholesterol acyl transferase activity was normal. Although the precise biochemical defect was not identified, there was a failure of transport of cholesterol from chylomicrons in vitro. We propose that the sea-blue histiocyte is a marker, in some cases, of abnormal lipid metabolism.", "PMID": 977773}
{"id": "pubmed23n0032_11347", "title": "Serum triiodothyronine determination in clinical use.", "content": "Two radioimmunassays for the determination of serum triiodothyronine (T3) were developed. The assay of T3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of T3 in serum from 117 patients requiring assessment of their thyroid status. In 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum T3 concentration was 1-92 (actual range 0-88-2-62) nmol/l. A significant inverse relationship was observed between the serum T3 level and the age of the subject. Serum total T3 levels discriminate clearly between hypo-,eu- and hyperthyroid patients and provide a rather more sensitive index of hyperthyroid function than total serum T4. In the face of normal serum T4 the T3 level was depressed in five patients with marked hypoproteinaemia and elevated in two patients taking heroin.", "contents": "Serum triiodothyronine determination in clinical use. Two radioimmunassays for the determination of serum triiodothyronine (T3) were developed. The assay of T3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of T3 in serum from 117 patients requiring assessment of their thyroid status. In 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum T3 concentration was 1-92 (actual range 0-88-2-62) nmol/l. A significant inverse relationship was observed between the serum T3 level and the age of the subject. Serum total T3 levels discriminate clearly between hypo-,eu- and hyperthyroid patients and provide a rather more sensitive index of hyperthyroid function than total serum T4. In the face of normal serum T4 the T3 level was depressed in five patients with marked hypoproteinaemia and elevated in two patients taking heroin.", "PMID": 977774}
{"id": "pubmed23n0032_11348", "title": "Severe selective IgM deficiency.", "content": "Among the primary antibody deficiency syndromes, severe selective IgM deficiency (also previously known as type V dysgammaglobulinaemia) is rare, and the majority of previous reports have indicated a fatal outcome. Three adult patients who were found to have a persistently low serum IgM are described. This deficiency was not obviously related to their presenting illness; in two of the patients, who were investigated in detail, it appeared to be of no immediate consequence.", "contents": "Severe selective IgM deficiency. Among the primary antibody deficiency syndromes, severe selective IgM deficiency (also previously known as type V dysgammaglobulinaemia) is rare, and the majority of previous reports have indicated a fatal outcome. Three adult patients who were found to have a persistently low serum IgM are described. This deficiency was not obviously related to their presenting illness; in two of the patients, who were investigated in detail, it appeared to be of no immediate consequence.", "PMID": 977778}
{"id": "pubmed23n0032_11349", "title": "Polyacrylamide gel disc electrophoresis of alkaline phosphatase isoenzymes in bone and liver disease.", "content": "Acrylamide gel disc electrophoresis provides a reliable and reasonably rapid method of differentiating the raised serum alkaline phosphatase (AP) of bone origin from that of liver origin. The technique has been placed for the first time on a semiquantitative basis. Measurement of both band width and band position effectively distinguishes the bone from the liver isoenzyme, but band width provides superior discrimination. An origin band was seen in none of the normal subjects and in only 7% of patients with bone disease but was present in 78% of patients with liver disease, a highly significant increase. Fifty percent of normal individuals had a small-intestinal band in serum taken two hours after a meal, as did 35% of patients with liver disease, but the incidence of intestinal bands in bone disease was only 11%, significantly less than in the other two groups. The genetic control of small-intestinal AP in serum has been confirmed, but it has been demonstrated that the decrease of intestinal AP in bone disorders is not genetically determined.", "contents": "Polyacrylamide gel disc electrophoresis of alkaline phosphatase isoenzymes in bone and liver disease. Acrylamide gel disc electrophoresis provides a reliable and reasonably rapid method of differentiating the raised serum alkaline phosphatase (AP) of bone origin from that of liver origin. The technique has been placed for the first time on a semiquantitative basis. Measurement of both band width and band position effectively distinguishes the bone from the liver isoenzyme, but band width provides superior discrimination. An origin band was seen in none of the normal subjects and in only 7% of patients with bone disease but was present in 78% of patients with liver disease, a highly significant increase. Fifty percent of normal individuals had a small-intestinal band in serum taken two hours after a meal, as did 35% of patients with liver disease, but the incidence of intestinal bands in bone disease was only 11%, significantly less than in the other two groups. The genetic control of small-intestinal AP in serum has been confirmed, but it has been demonstrated that the decrease of intestinal AP in bone disorders is not genetically determined.", "PMID": 977779}
{"id": "pubmed23n0032_11350", "title": "A case of veno-occlusive disease of the liver in Britain associated with herbal tea consumption.", "content": "Veno-occlusive disease of the liver with clinical and pathological features similar to those of the condition occurring in the Caribbean is described in a young woman resident in Britain. The diagnosis was made from liver biopsies and hepatic venography and was confirmed at necropsy. Small amounts of pyrroliziding alkaloids were recovered from a sample of mate (Paraguay) tea, owned by the patient, to which she was addicted. It seems probable that the consumption of large amounts of this tea over a period of years was the cause of the hepatic disease.", "contents": "A case of veno-occlusive disease of the liver in Britain associated with herbal tea consumption. Veno-occlusive disease of the liver with clinical and pathological features similar to those of the condition occurring in the Caribbean is described in a young woman resident in Britain. The diagnosis was made from liver biopsies and hepatic venography and was confirmed at necropsy. Small amounts of pyrroliziding alkaloids were recovered from a sample of mate (Paraguay) tea, owned by the patient, to which she was addicted. It seems probable that the consumption of large amounts of this tea over a period of years was the cause of the hepatic disease.", "PMID": 977780}
{"id": "pubmed23n0032_11351", "title": "Papillary tumours of the minor salivary glands.", "content": "The clinical and histological features of oncocytic adenomatous hyperplasia, papillary adenoma, and papillary adenocarcinoma of the oral cavity are described, and the literature is reviewed. Histological features which may be of value in distinguishing between benign and malignant variants are described, and in view of the slow growth rate of most of these tumours, the importance of long-term follow-up is stressed.", "contents": "Papillary tumours of the minor salivary glands. The clinical and histological features of oncocytic adenomatous hyperplasia, papillary adenoma, and papillary adenocarcinoma of the oral cavity are described, and the literature is reviewed. Histological features which may be of value in distinguishing between benign and malignant variants are described, and in view of the slow growth rate of most of these tumours, the importance of long-term follow-up is stressed.", "PMID": 977781}
{"id": "pubmed23n0032_11352", "title": "Pseudotumour of the lung caused by infection with Bacillus sphaericus.", "content": "A patient is described who suffered from severe chronic asthma complicated by repeated chest infections. She developed a large gelatinous pseudotumour of the lung which was found to be caused by Bacillus sphaericus.", "contents": "Pseudotumour of the lung caused by infection with Bacillus sphaericus. A patient is described who suffered from severe chronic asthma complicated by repeated chest infections. She developed a large gelatinous pseudotumour of the lung which was found to be caused by Bacillus sphaericus.", "PMID": 977782}
{"id": "pubmed23n0032_11353", "title": "Flavobacterium meningosepticum in the hospital environment.", "content": "Ten strains of Flavobacterium meningosepticum were isolated from routine clinical material during a period of two years. Of a number of antibiotics tested, clindamycin was the only one to which they were fully sensitive. The organism was found in chlorhexidine gluconate (Hibitane) solutions in use on the wards for the storage of thermometers and for routine disinfection.", "contents": "Flavobacterium meningosepticum in the hospital environment. Ten strains of Flavobacterium meningosepticum were isolated from routine clinical material during a period of two years. Of a number of antibiotics tested, clindamycin was the only one to which they were fully sensitive. The organism was found in chlorhexidine gluconate (Hibitane) solutions in use on the wards for the storage of thermometers and for routine disinfection.", "PMID": 977783}
{"id": "pubmed23n0032_11354", "title": "An assessment of inositol fermentation by Klebsiellae and its implication in screening media.", "content": "Four patterns of inositol fermentation by different isolates of Klebsiellae are described. Inositol fermentation by some isolates may be modified by medium incorporation of acetoin and diacetyl. An inositol--hydrogen sulphide--motility medium, modified by reducing the pH value, increasing the inositol concentration, and the incorporation of acetoin, is described and evaluated as a means of detecting acid production by isolates showing different inositol fermentation patterns.", "contents": "An assessment of inositol fermentation by Klebsiellae and its implication in screening media. Four patterns of inositol fermentation by different isolates of Klebsiellae are described. Inositol fermentation by some isolates may be modified by medium incorporation of acetoin and diacetyl. An inositol--hydrogen sulphide--motility medium, modified by reducing the pH value, increasing the inositol concentration, and the incorporation of acetoin, is described and evaluated as a means of detecting acid production by isolates showing different inositol fermentation patterns.", "PMID": 977784}
{"id": "pubmed23n0032_11355", "title": "Detection of staphylococcal enterotoxin in gastric juice.", "content": "The gastric juice of a patient showing symptoms of staphylococcal food poisoning was examined by a radioimmunoassay for the presence of enterotoxins. Assays gave markedly higher results at 35 degrees than at 5 degrees. The source for this discrepancy was attributed to interference due to trypsin activity on the basis of (1) the demonstration of hydrolysis of p-toluenesulfonyl-L-arginine methyl ester by the specimen, (2) inhibition of this activity by trypsin inhibitor from lima bean, and (3) lowered values produced for enterotoxins in gastric juice when the inhibitor was included in the assay system.", "contents": "Detection of staphylococcal enterotoxin in gastric juice. The gastric juice of a patient showing symptoms of staphylococcal food poisoning was examined by a radioimmunoassay for the presence of enterotoxins. Assays gave markedly higher results at 35 degrees than at 5 degrees. The source for this discrepancy was attributed to interference due to trypsin activity on the basis of (1) the demonstration of hydrolysis of p-toluenesulfonyl-L-arginine methyl ester by the specimen, (2) inhibition of this activity by trypsin inhibitor from lima bean, and (3) lowered values produced for enterotoxins in gastric juice when the inhibitor was included in the assay system.", "PMID": 977785}
{"id": "pubmed23n0032_11356", "title": "Rate of clearance by the spleen of heat-damaged erythrocytes.", "content": "The rate of uptake in the spleen of heat-damaged red blood cells labelled with 99mTc has been measured by radioisotope scanning in 38 patients. The values obtained for the half-clearance time into the spleen using this method have been compared with the values obtained simultaneously by blood sampling for the rate of loss of radioactivity from the circulation. It was found that the uptake into the spleen was approximately three times faster than the measured rate of loss of radioactivity from the blood. The reasons for this are discussed and it is suggested that the scanning technique is more reliable as a measure of this aspect of splenic function.", "contents": "Rate of clearance by the spleen of heat-damaged erythrocytes. The rate of uptake in the spleen of heat-damaged red blood cells labelled with 99mTc has been measured by radioisotope scanning in 38 patients. The values obtained for the half-clearance time into the spleen using this method have been compared with the values obtained simultaneously by blood sampling for the rate of loss of radioactivity from the circulation. It was found that the uptake into the spleen was approximately three times faster than the measured rate of loss of radioactivity from the blood. The reasons for this are discussed and it is suggested that the scanning technique is more reliable as a measure of this aspect of splenic function.", "PMID": 977786}
{"id": "pubmed23n0032_11357", "title": "A comparison of the bioavailability of digoxin in capsule, tablet, and solution taken orally with intravenous digoxin.", "content": "Six healthy volunteers were given five single-dose treatments of 0.40 mg digoxin either intravenously, in liquid form, in conventional tablet form (dissolution rate 76 per cent in 1-hour), or in new capsule preparations containing 0.05, 0.10, or 0.20 mg digoxin per capsule. Serum levels, area under the concentration-time curve, and daily urinary digoxin excretion were measured for six days. Higher serum digoxin levels were seen after ingestion of the capsules than after the tablets, with peak levels for the former being 2.2-2.8 times higher than after tablet digoxin. Bioavailability was assessed further by comparing the area under a six-hour concentration-time curve, and again the capsules gave a consistently higher value than the tablets. In addition, the absorption of 0.40 mg digoxin from any of the capsule preparations was much greater than 0.50 mg digoxin in commercially available tablets. The six-day cumulative urinary digoxin excretion was also greater for the capsules than for the 0.20-mg tablets. In comparison with intravenous digoxin, tablets provide 75 per cent maximum bioavailability, whereas the capsule preparations of digoxin improve the bioavailability of digoxin and the 0.20-mg digoxin capsule is absorbed better than 0.25-mg digoxin tablet.", "contents": "A comparison of the bioavailability of digoxin in capsule, tablet, and solution taken orally with intravenous digoxin. Six healthy volunteers were given five single-dose treatments of 0.40 mg digoxin either intravenously, in liquid form, in conventional tablet form (dissolution rate 76 per cent in 1-hour), or in new capsule preparations containing 0.05, 0.10, or 0.20 mg digoxin per capsule. Serum levels, area under the concentration-time curve, and daily urinary digoxin excretion were measured for six days. Higher serum digoxin levels were seen after ingestion of the capsules than after the tablets, with peak levels for the former being 2.2-2.8 times higher than after tablet digoxin. Bioavailability was assessed further by comparing the area under a six-hour concentration-time curve, and again the capsules gave a consistently higher value than the tablets. In addition, the absorption of 0.40 mg digoxin from any of the capsule preparations was much greater than 0.50 mg digoxin in commercially available tablets. The six-day cumulative urinary digoxin excretion was also greater for the capsules than for the 0.20-mg tablets. In comparison with intravenous digoxin, tablets provide 75 per cent maximum bioavailability, whereas the capsule preparations of digoxin improve the bioavailability of digoxin and the 0.20-mg digoxin capsule is absorbed better than 0.25-mg digoxin tablet.", "PMID": 977789}
{"id": "pubmed23n0032_11358", "title": "Influence of renal dysfunction on warfarin plasma protein binding.", "content": "The in vitro plasma protein binding of warfarin was evaluated by ultrafiltration in plasma from seven normal subjects and seven patients with varying degrees of renal dysfunction. The unbound, or free, warfarin fraction was twice as high in the plasma samples withdrawn from patients with renal impairment than in the samples obtained from normal volunteers. Furthermore, there was a positive correlation between the extent of renal dysfunction and the magnitude of the free warfarin fraction when either creatinine clearance, plasma creatinine, or BUN was used to estimate renal function.", "contents": "Influence of renal dysfunction on warfarin plasma protein binding. The in vitro plasma protein binding of warfarin was evaluated by ultrafiltration in plasma from seven normal subjects and seven patients with varying degrees of renal dysfunction. The unbound, or free, warfarin fraction was twice as high in the plasma samples withdrawn from patients with renal impairment than in the samples obtained from normal volunteers. Furthermore, there was a positive correlation between the extent of renal dysfunction and the magnitude of the free warfarin fraction when either creatinine clearance, plasma creatinine, or BUN was used to estimate renal function.", "PMID": 977790}
{"id": "pubmed23n0032_11359", "title": "The effects of chlordesmethyldiazepam on behavioral performance and subjective judgment in normal subjects.", "content": "Eight normal subjects were tested on a battery of subjective and psychological tests 12 hours after a hypnotic dose of chlordesmethyldiazepam (1 or 2 mg), amylobarbitone sodium (100 mg), and a placebo. The tests included self-ratings of hypnotic effects and alertness; reactiontime, card-sorting, coding and cancellation tasks; arithmetic; and tapping. Before each task, test anxiety and performance expectation was self-rated; and after the task, judgment of performance was self-rated. Residual effects were definitely detectable after the 2-mg dose of benzodiazepine with both behavioral impairment and subjective hangover. Both the 1-mg dose and the barbiturate were almost devoid of such effects. Very few drug effects on test anxiety and performance judgment were discerned. Plasma concentrations of amylobarbitone were related to decreases in test anxiety and of chlordesmethyldiazepam with ratings of sleepiness.", "contents": "The effects of chlordesmethyldiazepam on behavioral performance and subjective judgment in normal subjects. Eight normal subjects were tested on a battery of subjective and psychological tests 12 hours after a hypnotic dose of chlordesmethyldiazepam (1 or 2 mg), amylobarbitone sodium (100 mg), and a placebo. The tests included self-ratings of hypnotic effects and alertness; reactiontime, card-sorting, coding and cancellation tasks; arithmetic; and tapping. Before each task, test anxiety and performance expectation was self-rated; and after the task, judgment of performance was self-rated. Residual effects were definitely detectable after the 2-mg dose of benzodiazepine with both behavioral impairment and subjective hangover. Both the 1-mg dose and the barbiturate were almost devoid of such effects. Very few drug effects on test anxiety and performance judgment were discerned. Plasma concentrations of amylobarbitone were related to decreases in test anxiety and of chlordesmethyldiazepam with ratings of sleepiness.", "PMID": 977791}
{"id": "pubmed23n0032_11360", "title": "The utility of pharmacokinetics to the pharmaceutical industry.", "content": "Pharmacokinetic studies done early in the course of drug development can favorably influence the course of research by suggesting optimum dosage regimens, drug delivery methods, and fixed-ratio combinations and by alerting the clinical pharmacologist to potential problems. Pharmacokinetic data can be useful in the design of rational clinical drug research and thereby expedite clinical trials and save research costs. Thus, in addition to their value in the area of comparative bio-availability, pharmacokinetic studies carried out by the pharmaceutical industry are useful economically as well as scientifically.", "contents": "The utility of pharmacokinetics to the pharmaceutical industry. Pharmacokinetic studies done early in the course of drug development can favorably influence the course of research by suggesting optimum dosage regimens, drug delivery methods, and fixed-ratio combinations and by alerting the clinical pharmacologist to potential problems. Pharmacokinetic data can be useful in the design of rational clinical drug research and thereby expedite clinical trials and save research costs. Thus, in addition to their value in the area of comparative bio-availability, pharmacokinetic studies carried out by the pharmaceutical industry are useful economically as well as scientifically.", "PMID": 977797}
{"id": "pubmed23n0032_11361", "title": "Comparison of hospital admissions for cerebrovascular disease in Michigan and North Carolina.", "content": "Hospital admission rates and death rates for cerebrovascular disease differ markedly between states. Hospital admission data were analyzed: (a) to ascertain why death rates from this disease group are lower in Michigan than in North Carolina and (b) to clarify whether hospital care in either state has inadequacies that can be corrected quickly. Among both whites and blacks of the same age, case-fatality ratios were higher in North Carolina than in Michigan. Subarachnoid and cerebral hemorrhages were diagnosed more often in the southern state. For both areas, the records showed a marked underreporting of hypertension and diabetes mellitus as secondary conditions in the hospital admissions; elevated blood pressures were about equally common in each state but were treated more vigorously in Michigan. Secondary diagnoses of respiratory disease and use of anti-infective agents were reported more frequently in North Carolina. In contrast, diabetes mellitus was more prevalent in Michigan admissions. Some reasons for these findings are advanced, particularly as they relate to diagnostic and treatment patterns. Finally, the need for more detailed research is emphasized to create guidelines for better hospital care of cerebrovascular disease.", "contents": "Comparison of hospital admissions for cerebrovascular disease in Michigan and North Carolina. Hospital admission rates and death rates for cerebrovascular disease differ markedly between states. Hospital admission data were analyzed: (a) to ascertain why death rates from this disease group are lower in Michigan than in North Carolina and (b) to clarify whether hospital care in either state has inadequacies that can be corrected quickly. Among both whites and blacks of the same age, case-fatality ratios were higher in North Carolina than in Michigan. Subarachnoid and cerebral hemorrhages were diagnosed more often in the southern state. For both areas, the records showed a marked underreporting of hypertension and diabetes mellitus as secondary conditions in the hospital admissions; elevated blood pressures were about equally common in each state but were treated more vigorously in Michigan. Secondary diagnoses of respiratory disease and use of anti-infective agents were reported more frequently in North Carolina. In contrast, diabetes mellitus was more prevalent in Michigan admissions. Some reasons for these findings are advanced, particularly as they relate to diagnostic and treatment patterns. Finally, the need for more detailed research is emphasized to create guidelines for better hospital care of cerebrovascular disease.", "PMID": 977805}
{"id": "pubmed23n0032_11362", "title": "Does level of professional training make a difference in crisis intervention counseling?", "content": "This study tests the concept that crisis intervention using a multilevel trained staff is more effective, because a staff with less education might better reach clients from diverse socioeconomic and cultural backgrounds. The Client-Post Rating Scale (Revised) was administered to a sample of clients served by a multilevel crisis intervention team located in a large midwestern metropolitan area to determine the efficacy of worker intervention. It was found that level of training was unrelated to counselor effectiveness and that less well-educated staff more frequently worked with less economically advantaged populations.", "contents": "Does level of professional training make a difference in crisis intervention counseling? This study tests the concept that crisis intervention using a multilevel trained staff is more effective, because a staff with less education might better reach clients from diverse socioeconomic and cultural backgrounds. The Client-Post Rating Scale (Revised) was administered to a sample of clients served by a multilevel crisis intervention team located in a large midwestern metropolitan area to determine the efficacy of worker intervention. It was found that level of training was unrelated to counselor effectiveness and that less well-educated staff more frequently worked with less economically advantaged populations.", "PMID": 977806}
{"id": "pubmed23n0032_11363", "title": "The policy effectiveness of health services research: a reconsideration.", "content": "It has often been asserted that health services research (HSR) has been either epiphenomenal or entirely ineffectual in influencing the formation of policy. In this examination of the HSR-policy relationship that assertion is contested; the authors conclude that there has been a relationship between HSR and policy, at least public policy. A diagrammatic framework is presented to describe the HSR-policy nexus in an attempt to facilitate an understanding of how future efforts in the conduct of HSR might be most effective.", "contents": "The policy effectiveness of health services research: a reconsideration. It has often been asserted that health services research (HSR) has been either epiphenomenal or entirely ineffectual in influencing the formation of policy. In this examination of the HSR-policy relationship that assertion is contested; the authors conclude that there has been a relationship between HSR and policy, at least public policy. A diagrammatic framework is presented to describe the HSR-policy nexus in an attempt to facilitate an understanding of how future efforts in the conduct of HSR might be most effective.", "PMID": 977807}
{"id": "pubmed23n0032_11364", "title": "The role of the health facilitator in community health education.", "content": "A community health education program has been established by the Department of Community Health Sciences of Duke University Medical Center in two target areas of Durham County, North Carolina. The program trains unpaid lay people, \"health facilitators\", to whom others already turn for help, to increase their competency for advising and referring community residents to appropriate community resources. Several methods for identifying potential health facilitators have been developed. Evaluation of the role of health facilitators hinges upon designing measures to demonstrate their existence in the community and the impact that they make. To be successful, the training of health facilitators should result in improving the quality of the advice they give their fellows. It is also important to assess the cultural acceptability of the facilitators' advice and to ensure that their helping role in the community is not disturbed by the possible professionalization of their role due to the training program.", "contents": "The role of the health facilitator in community health education. A community health education program has been established by the Department of Community Health Sciences of Duke University Medical Center in two target areas of Durham County, North Carolina. The program trains unpaid lay people, \"health facilitators\", to whom others already turn for help, to increase their competency for advising and referring community residents to appropriate community resources. Several methods for identifying potential health facilitators have been developed. Evaluation of the role of health facilitators hinges upon designing measures to demonstrate their existence in the community and the impact that they make. To be successful, the training of health facilitators should result in improving the quality of the advice they give their fellows. It is also important to assess the cultural acceptability of the facilitators' advice and to ensure that their helping role in the community is not disturbed by the possible professionalization of their role due to the training program.", "PMID": 977808}
{"id": "pubmed23n0032_11365", "title": "Problems in the development of a rural primary care center.", "content": "For 18 months between July 1973 and December 1974, the development of a primary care center for a rural Appalacian county engaged the efforts of local community leaders and the Department of Community Medicine of the University of Kentucky College of Medicine. This case study discusses some of the major events and factors contributing to this unsuccessful endeavor. The local cultural and sociomedical circumstances, including economic and political forces, and the errors in institutional relations and communication are analyzed. Many of the community problems encountered, at both the local and regional levels, are common to various rural areas. Considerations discussed in some detail include medical care payment mechanisms, rural economics, and local sociocultural systems. These community health problems, and the broader health policy issues that they represent, must be addressed before any significant changes or advances can be made in health care in rural America.", "contents": "Problems in the development of a rural primary care center. For 18 months between July 1973 and December 1974, the development of a primary care center for a rural Appalacian county engaged the efforts of local community leaders and the Department of Community Medicine of the University of Kentucky College of Medicine. This case study discusses some of the major events and factors contributing to this unsuccessful endeavor. The local cultural and sociomedical circumstances, including economic and political forces, and the errors in institutional relations and communication are analyzed. Many of the community problems encountered, at both the local and regional levels, are common to various rural areas. Considerations discussed in some detail include medical care payment mechanisms, rural economics, and local sociocultural systems. These community health problems, and the broader health policy issues that they represent, must be addressed before any significant changes or advances can be made in health care in rural America.", "PMID": 977809}
{"id": "pubmed23n0032_11366", "title": "The localization of cholinesterase in the retina of the fetal and newborn guinea pig.", "content": "Retinae of guinea pigs from the fortieth day of gestation to one day postnatally were processed for the localization of cholinesterases in the electron microscope according to the method of Lewis and Shute ('66). Selective inhibition served to distinguish acetylcholinesterase from non-specific cholinesterase activity. Acetylcholinesterase activity was found initially in small amounts in some regions of the outer plexiform layer at the fortieth day of gestation. At later stages it increased in distribution being observed at some photoreceptor terminals and in non-synaptic regions of the layer. Activity was less intense initially in the inner plexiform layer but increased rapidly so that by birth it encompassed a majority of processes. Perikarya of horizontal and some amacrine and ganglion cells possessed acetylcholinesterase activity in their nuclear envelope and rough endoplasmic reticulum. The possible role of the enzyme in inhibitory circuits of the fetal retina is discussed.", "contents": "The localization of cholinesterase in the retina of the fetal and newborn guinea pig. Retinae of guinea pigs from the fortieth day of gestation to one day postnatally were processed for the localization of cholinesterases in the electron microscope according to the method of Lewis and Shute ('66). Selective inhibition served to distinguish acetylcholinesterase from non-specific cholinesterase activity. Acetylcholinesterase activity was found initially in small amounts in some regions of the outer plexiform layer at the fortieth day of gestation. At later stages it increased in distribution being observed at some photoreceptor terminals and in non-synaptic regions of the layer. Activity was less intense initially in the inner plexiform layer but increased rapidly so that by birth it encompassed a majority of processes. Perikarya of horizontal and some amacrine and ganglion cells possessed acetylcholinesterase activity in their nuclear envelope and rough endoplasmic reticulum. The possible role of the enzyme in inhibitory circuits of the fetal retina is discussed.", "PMID": 977811}
{"id": "pubmed23n0032_11367", "title": "The development of the cerebellar cortex of the Syrian hamster, Mesocricetus auratus. Foliation, cytoarchitectonic, Golgi, and electron microscopic studies.", "content": "Although a number of investigations of abnormalities of cerebellar development have been carried out in the hamster, no detailed Golgi or ultrastructural studies of cerebellar development in this species have been reported. This report describes the development of the hamster cerebellar cortex from birth (day 0) through postnatal day 78, as studied by light, Golgi, and electron microscopic methods. Foliation patterns correlate with the expansion of the cerebellar layers and of total cerebellar area. Cytologic and morphologic development of the major cerebellar cell types--Purkinje, Golgi, basket, stellate, granule, and Bergmann glial cells--correlate with those of other species, such as the rat and mouse. Electron microscopic observations at selected developmental ages allow identification and classification of synapses in the early postnatal hamster. Parallel fiber and climbing fiber synapses are already present at birth. Although synaptogenesis probably continues through the first two postnatal months, all major cell types have developed initial synapses by postnatal day 6, at a time when little cellular maturation has occurred. By using gestational rather than natal age, close developmental correlations between hamsters and rat and mouse are possible. Since the gestational period of the hamster is only 16 days, the hamster cerebellum is less mature at birth than that of either the rat or mouse. Thus, the hamster is a convenient animal in which to investigate the effects of various procedures on early cerebellar development.", "contents": "The development of the cerebellar cortex of the Syrian hamster, Mesocricetus auratus. Foliation, cytoarchitectonic, Golgi, and electron microscopic studies. Although a number of investigations of abnormalities of cerebellar development have been carried out in the hamster, no detailed Golgi or ultrastructural studies of cerebellar development in this species have been reported. This report describes the development of the hamster cerebellar cortex from birth (day 0) through postnatal day 78, as studied by light, Golgi, and electron microscopic methods. Foliation patterns correlate with the expansion of the cerebellar layers and of total cerebellar area. Cytologic and morphologic development of the major cerebellar cell types--Purkinje, Golgi, basket, stellate, granule, and Bergmann glial cells--correlate with those of other species, such as the rat and mouse. Electron microscopic observations at selected developmental ages allow identification and classification of synapses in the early postnatal hamster. Parallel fiber and climbing fiber synapses are already present at birth. Although synaptogenesis probably continues through the first two postnatal months, all major cell types have developed initial synapses by postnatal day 6, at a time when little cellular maturation has occurred. By using gestational rather than natal age, close developmental correlations between hamsters and rat and mouse are possible. Since the gestational period of the hamster is only 16 days, the hamster cerebellum is less mature at birth than that of either the rat or mouse. Thus, the hamster is a convenient animal in which to investigate the effects of various procedures on early cerebellar development.", "PMID": 977812}
{"id": "pubmed23n0032_11368", "title": "The structural maturation of the stato-acoustic nerve in the cat.", "content": "The maturation of the stato-acoustic nerve in the cat was studied by light and electron microscopy from the fetal stage to the adult. Measurement of the outer diameter of the fibers and the study of the myelination process revealed that myelination begins earlier for the vestibular nerve than for the cochlear nerve: by the fifty-third day of gestation 64% of the vestibular fibres have already passed the promyelin stage whereas for the cochlear nerve this promyelin stage begins for the majority of fibers on the fifty-seventh gestation day. Afterward, maturation proceeds more rapidly for the cochlear nerve. In the case of both nerves, maturation is still incomplete at two months of age. Concerning the relationship between the thickness of the myelin sheath and the axoplasmic diameter, there is already a good correlation by the fifty-seventh day of gestation in the vestibular nerve, whereas it appears several days after birth in the cochlear nerve.", "contents": "The structural maturation of the stato-acoustic nerve in the cat. The maturation of the stato-acoustic nerve in the cat was studied by light and electron microscopy from the fetal stage to the adult. Measurement of the outer diameter of the fibers and the study of the myelination process revealed that myelination begins earlier for the vestibular nerve than for the cochlear nerve: by the fifty-third day of gestation 64% of the vestibular fibres have already passed the promyelin stage whereas for the cochlear nerve this promyelin stage begins for the majority of fibers on the fifty-seventh gestation day. Afterward, maturation proceeds more rapidly for the cochlear nerve. In the case of both nerves, maturation is still incomplete at two months of age. Concerning the relationship between the thickness of the myelin sheath and the axoplasmic diameter, there is already a good correlation by the fifty-seventh day of gestation in the vestibular nerve, whereas it appears several days after birth in the cochlear nerve.", "PMID": 977813}
{"id": "pubmed23n0032_11369", "title": "Evidence that at least some of the motor nerve cells that die during development have first made peripheral connections.", "content": "The paper sets out to determine whether the spinal motoneurons that die during the normal development of Xenopus tadpoles have been peripherally connected before death. The numbers of cells in the control ventral horns were compared with the numbers remaining after early (stage 50 or 52/53) amputation of the limb bud. Up to stage 52, there was no difference between the two sides, but thereafter the numbers on the amputated side fell away until at stage 57 none were left. Thus, as neurons mature, they become dependent for survival on contacting the limb. During this time, there was no difference between amputees and controls in the numbers of mitoses in that part of the ventricular layer that gives rise to ventral horn cells, indicating no change in proliferation. Thus, the numbers of ventral horn cells remaining after early amputation is a measure of the numbers of cells in the normal animal that are still independent of the limb (Phase I cells) and hence by subtraction, the other cells (post-Phase I cells) are those that only survive by virtue of having contacted the limn. A graph of numbers of post-Phase I cells shows a peak at stage 54 and thereafter declines as metamorphosis approaches. This is interpreted to indicate that a number of cells that survive at stage 54 by reason of peripheral contacts or connections in the limb subsequently themselves die, that is, that al least some of the cells that die during normal development have previously made contacts within the limb.", "contents": "Evidence that at least some of the motor nerve cells that die during development have first made peripheral connections. The paper sets out to determine whether the spinal motoneurons that die during the normal development of Xenopus tadpoles have been peripherally connected before death. The numbers of cells in the control ventral horns were compared with the numbers remaining after early (stage 50 or 52/53) amputation of the limb bud. Up to stage 52, there was no difference between the two sides, but thereafter the numbers on the amputated side fell away until at stage 57 none were left. Thus, as neurons mature, they become dependent for survival on contacting the limb. During this time, there was no difference between amputees and controls in the numbers of mitoses in that part of the ventricular layer that gives rise to ventral horn cells, indicating no change in proliferation. Thus, the numbers of ventral horn cells remaining after early amputation is a measure of the numbers of cells in the normal animal that are still independent of the limb (Phase I cells) and hence by subtraction, the other cells (post-Phase I cells) are those that only survive by virtue of having contacted the limn. A graph of numbers of post-Phase I cells shows a peak at stage 54 and thereafter declines as metamorphosis approaches. This is interpreted to indicate that a number of cells that survive at stage 54 by reason of peripheral contacts or connections in the limb subsequently themselves die, that is, that al least some of the cells that die during normal development have previously made contacts within the limb.", "PMID": 977814}
{"id": "pubmed23n0032_11370", "title": "Areal changes in mouse cortical barrels following vibrissal damage at different postnatal ages.", "content": "The normal cytoarchitectonic pattern of barrels in layer IV of mouse SmI face cortex is altered by early damage to the mystacial vibrissae (Van der Loos and Woolsey, '73). In the present study, the middle row of vibrissae (row-C) on one side of the face in groups of Swiss mice was cauterized on the day of birth (postnatal day [PND] -1)or on PND's - 2, 3, 4, 5, 7, 10, 12, 15, 20 and 30; littermates in each group served as controls. All animals were perfused on PND-60 and the brains sectioned parallel to SmI layer IV. For each specimen, the posteromedial barrel subfields (PMBSF) of the two hemispheres were reconstructed with a camera lucida and the cross-sectional areas of individual barrels measured using a small computer. The findings are: (1) The hemispheres ipsilateral to the vibrissal damage are quantitatively indistinguishable from the littermate controls indicating that the ipsilateral hemispheres in lesioned animals can serve as controls for observations of the type reported in this paper. (2) There are no consistent differences in the cross-sectional areas of the PMBSF's as a whole in the hemispheres ipsi- and contralateral to the peripheral damage, suggesting that there is no net loss of cortex as a result of the lesions. (3) The contralateral row-C barrels are reduced in size. Expressed as a percentage of normal values; row-C is reduced to 17% for animals lesioned on PND-1, 16% on PND-2, 38% on PND-3, 52% on PND-4 and 79% on PND-5; on PND-7 and later the cross-sectional areas of row-C barrels are normal. This implies that the barrel field of the SmI face cortex becomes progressively refractory to the effects of peripheral damage during the first postnatal week and in the period prior to PND-6, an intact periphery is necessary for normal cortical development. (4) In every case, the decreased cross-sectional area of row-C is accompanied by precisely increased cross-sectional areas of the barrels in adjacent rows-B and D. in the case of the restricted peripheral damage which we produced, there is a \"compensation\" in the contralateral hemisphere, which can be correlated with patterns of the specific thalamocortical projections.", "contents": "Areal changes in mouse cortical barrels following vibrissal damage at different postnatal ages. The normal cytoarchitectonic pattern of barrels in layer IV of mouse SmI face cortex is altered by early damage to the mystacial vibrissae (Van der Loos and Woolsey, '73). In the present study, the middle row of vibrissae (row-C) on one side of the face in groups of Swiss mice was cauterized on the day of birth (postnatal day [PND] -1)or on PND's - 2, 3, 4, 5, 7, 10, 12, 15, 20 and 30; littermates in each group served as controls. All animals were perfused on PND-60 and the brains sectioned parallel to SmI layer IV. For each specimen, the posteromedial barrel subfields (PMBSF) of the two hemispheres were reconstructed with a camera lucida and the cross-sectional areas of individual barrels measured using a small computer. The findings are: (1) The hemispheres ipsilateral to the vibrissal damage are quantitatively indistinguishable from the littermate controls indicating that the ipsilateral hemispheres in lesioned animals can serve as controls for observations of the type reported in this paper. (2) There are no consistent differences in the cross-sectional areas of the PMBSF's as a whole in the hemispheres ipsi- and contralateral to the peripheral damage, suggesting that there is no net loss of cortex as a result of the lesions. (3) The contralateral row-C barrels are reduced in size. Expressed as a percentage of normal values; row-C is reduced to 17% for animals lesioned on PND-1, 16% on PND-2, 38% on PND-3, 52% on PND-4 and 79% on PND-5; on PND-7 and later the cross-sectional areas of row-C barrels are normal. This implies that the barrel field of the SmI face cortex becomes progressively refractory to the effects of peripheral damage during the first postnatal week and in the period prior to PND-6, an intact periphery is necessary for normal cortical development. (4) In every case, the decreased cross-sectional area of row-C is accompanied by precisely increased cross-sectional areas of the barrels in adjacent rows-B and D. in the case of the restricted peripheral damage which we produced, there is a \"compensation\" in the contralateral hemisphere, which can be correlated with patterns of the specific thalamocortical projections.", "PMID": 977815}
{"id": "pubmed23n0032_11371", "title": "Single unit studies on the dorsal and intermediate acoustic striae.", "content": "Studies were made of the fiber compositon and of single unit dischage characteristics in the dorsal and intermediate acoustic straie. Counts of fibers and cells revealed more than twice as many fibers as cochlear nucleus cells of the types known to contribute axons to these tracts. Recordings were made in the striae to confirm the classes of discharge patterns which are transmitted from the posterior cochlear nucleus to more central structures and to seek evidence for descending activity entering the cochlear nucleus through the striae. Discharge patterns of the majority of units resembled those seen in previous studies when recordings were made from regions of the cochlear nucleus which are known to contribute fibers to the striae. Some patterns, however, were unlike any which have been reported in the cochlear nucleus on the same side as the recording site, but resembled activity of other auditory structures, suggesting that these were descending inputs. To confirm the presence of descending activity, recordings were made in the dorsal acoustic stria at a point medial to where the stria had been completely severed. Recordings made under these conditions demonstrated the presence of descending activity. The physiological findings help explain the disparity between the counts of fibers and cells, and point out a need for more detailed descriptions of the origins of descending inputs through the striae.", "contents": "Single unit studies on the dorsal and intermediate acoustic striae. Studies were made of the fiber compositon and of single unit dischage characteristics in the dorsal and intermediate acoustic straie. Counts of fibers and cells revealed more than twice as many fibers as cochlear nucleus cells of the types known to contribute axons to these tracts. Recordings were made in the striae to confirm the classes of discharge patterns which are transmitted from the posterior cochlear nucleus to more central structures and to seek evidence for descending activity entering the cochlear nucleus through the striae. Discharge patterns of the majority of units resembled those seen in previous studies when recordings were made from regions of the cochlear nucleus which are known to contribute fibers to the striae. Some patterns, however, were unlike any which have been reported in the cochlear nucleus on the same side as the recording site, but resembled activity of other auditory structures, suggesting that these were descending inputs. To confirm the presence of descending activity, recordings were made in the dorsal acoustic stria at a point medial to where the stria had been completely severed. Recordings made under these conditions demonstrated the presence of descending activity. The physiological findings help explain the disparity between the counts of fibers and cells, and point out a need for more detailed descriptions of the origins of descending inputs through the striae.", "PMID": 977816}
{"id": "pubmed23n0032_11372", "title": "Mechanisms of efferent neuronal control of the reflex nicitating membrane response in rabbit (Oryctolagus cuniculus)", "content": "Efferent mechanisms controlling the nicitating membrane (NM) reflex response to air puff in the albino rabbit were analyzed using stimulation, lesions, and recording techniques. In brief, stimulation of the sixth nerve (abducens) yields short-latency NM extension. Stimulation of the fourth and seventh nerves and the superior cervical ganglion has essentially no effect on the NM. Stimulation of the third nerve causes short-latency retraction of the NM. Lesions and recording data are consistent with this result - the sole efferent neuronal control of NM extension is the sixth cranial nerve and of NM retraction is the third cranial nerve. The NM extension response appears to be mediated by mechanical actions via retraction of the eyeball by the retractor bulbi muscle, and NM retraction appears to result from direct activation of muscle fibers in the NM by the third nerve. The superior cervical ganglion appears to play no role in reflex NM retraction in the rabbit, in contrast to its action in the cat.", "contents": "Mechanisms of efferent neuronal control of the reflex nicitating membrane response in rabbit (Oryctolagus cuniculus). Efferent mechanisms controlling the nicitating membrane (NM) reflex response to air puff in the albino rabbit were analyzed using stimulation, lesions, and recording techniques. In brief, stimulation of the sixth nerve (abducens) yields short-latency NM extension. Stimulation of the fourth and seventh nerves and the superior cervical ganglion has essentially no effect on the NM. Stimulation of the third nerve causes short-latency retraction of the NM. Lesions and recording data are consistent with this result - the sole efferent neuronal control of NM extension is the sixth cranial nerve and of NM retraction is the third cranial nerve. The NM extension response appears to be mediated by mechanical actions via retraction of the eyeball by the retractor bulbi muscle, and NM retraction appears to result from direct activation of muscle fibers in the NM by the third nerve. The superior cervical ganglion appears to play no role in reflex NM retraction in the rabbit, in contrast to its action in the cat.", "PMID": 977817}
{"id": "pubmed23n0032_11373", "title": "Tone-induced changes in excitability of abducens motoneurons and of the reflex path of nictitating membrane response in rabbit (Oryctolagus cuniculus).", "content": "Excitability of the reflex nicitating membrane (NM) response to air puff and of the abducens motoneurons (final common path) to direct electrical stimulation (measured as amplitude of the evoked NM response) in rabbit was tested at various times after onset and offset of a 350-msec tone. Excitability to air puff showed a substantial increase during tone on a gradual decrease following tone off, in agreement with Ison and Leonard. Excitability of motoneurons showed a similar marked increase during tone on a transient decrease immediately following tone off, and then a gradual decrease. It is suggested that these excitability changes, which parallel closely the interstimulus-interval conditionability function reported by Gormezano for the same preparation, may provide an independent measure of the \"molar stimulus trace\". Excitability of abducens motoneurons tested in the intertrial intervals during subsequent tone-air puff conditioning showed no consistent changes. Surprisingly, animals given tone-abducens nucleus shock testing developed conditioned responses. In subsequent conditioning, using standard tone-air puff training, they showed 85% savings in acquisition relative to nonstimulated controls.", "contents": "Tone-induced changes in excitability of abducens motoneurons and of the reflex path of nictitating membrane response in rabbit (Oryctolagus cuniculus). Excitability of the reflex nicitating membrane (NM) response to air puff and of the abducens motoneurons (final common path) to direct electrical stimulation (measured as amplitude of the evoked NM response) in rabbit was tested at various times after onset and offset of a 350-msec tone. Excitability to air puff showed a substantial increase during tone on a gradual decrease following tone off, in agreement with Ison and Leonard. Excitability of motoneurons showed a similar marked increase during tone on a transient decrease immediately following tone off, and then a gradual decrease. It is suggested that these excitability changes, which parallel closely the interstimulus-interval conditionability function reported by Gormezano for the same preparation, may provide an independent measure of the \"molar stimulus trace\". Excitability of abducens motoneurons tested in the intertrial intervals during subsequent tone-air puff conditioning showed no consistent changes. Surprisingly, animals given tone-abducens nucleus shock testing developed conditioned responses. In subsequent conditioning, using standard tone-air puff training, they showed 85% savings in acquisition relative to nonstimulated controls.", "PMID": 977818}
{"id": "pubmed23n0032_11374", "title": "Peripheral heat as a reward for heart rate response in the curarized rat.", "content": "It is generally assumed that the mechanical perception of shivering is necessary for the perception of cold discomfort. Shivering of rats in a cool environment was eliminated by curarization. The rats were kept alive by artificial respiration. Heart rate was proportional to rectal temperature in a group of controls. One group of rats was conditioned to increase heart rate to trigger an infrared lamp; another group was conditioned to decrease heart rate to obtain heat. When compared with the results of the control group without infrared heat reward, the results obtained from the two heart-rate-modifying groups show that shivering is not a necessary signal to determine thermoregulatory behavior in rats and, presumably, cold discomfort in man.", "contents": "Peripheral heat as a reward for heart rate response in the curarized rat. It is generally assumed that the mechanical perception of shivering is necessary for the perception of cold discomfort. Shivering of rats in a cool environment was eliminated by curarization. The rats were kept alive by artificial respiration. Heart rate was proportional to rectal temperature in a group of controls. One group of rats was conditioned to increase heart rate to trigger an infrared lamp; another group was conditioned to decrease heart rate to obtain heat. When compared with the results of the control group without infrared heat reward, the results obtained from the two heart-rate-modifying groups show that shivering is not a necessary signal to determine thermoregulatory behavior in rats and, presumably, cold discomfort in man.", "PMID": 977819}
{"id": "pubmed23n0032_11375", "title": "Bait shyness: avoidance of the taste without escape from the illness in rats.", "content": "Thirsty rats habituated to drinking .12 M sodium chloride accepted .12 M lithium chloride for 5 min on the first trial but stopped short of their sodium baseline. With repeated trials they reduced consumption of the toxin by either (a) detecting subtle oral (conditioned stimulus, CS) differences, thus avoiding toxicosis (unconditioned stimulus, US) or (b) detecting earlier signs of malaise (US), thus escaping further distress. When both solutions were masked with saccharin, the discrimination was more difficult but still possible. When both solutions were mixed in a solution masking all four taste qualities, the discrimination was severely disrupted. When oral sensors were bypassed with nasopharyngeal tubes, intragastric pumping rats were unable to use postingestional cues to escape, even though such cues were proximal to the ultimate malaise. Oral cues at the distal end of the consummatory chain were extremely effective.", "contents": "Bait shyness: avoidance of the taste without escape from the illness in rats. Thirsty rats habituated to drinking .12 M sodium chloride accepted .12 M lithium chloride for 5 min on the first trial but stopped short of their sodium baseline. With repeated trials they reduced consumption of the toxin by either (a) detecting subtle oral (conditioned stimulus, CS) differences, thus avoiding toxicosis (unconditioned stimulus, US) or (b) detecting earlier signs of malaise (US), thus escaping further distress. When both solutions were masked with saccharin, the discrimination was more difficult but still possible. When both solutions were mixed in a solution masking all four taste qualities, the discrimination was severely disrupted. When oral sensors were bypassed with nasopharyngeal tubes, intragastric pumping rats were unable to use postingestional cues to escape, even though such cues were proximal to the ultimate malaise. Oral cues at the distal end of the consummatory chain were extremely effective.", "PMID": 977820}
{"id": "pubmed23n0032_11376", "title": "Role of mate's previous experience in ring dove hormone-induced incubation.", "content": "Progesterone-induced incubation was examined in 40 pairs of adult ring doves, differing in their previous reproductive experience (courtship vs. nest building) and the previous reproductive experience of their mates (courtship vs. nest building) during testing. Only the previous experience of the experimental subject significantly affected the establishment of incubation during the 24-hr test period. More doves experienced with nest building established incubation than did courtship-experienced doves. The latency to enter the nest area and the latency to stand on the nest were affected by both the previous breeding experience of the subject and the previous breeding experience of its mate. Nest-building-experienced mates decreased the latencies of courtship-experienced subjects, and courtship-experienced mates increased the latencies of nest-building-experienced doves. Males were not more affected by their mates' experience than were females. Time spent on the nest was affected only by the subject's previous experience and sex. While the hypothesis that the behavior of the mate can affect a dove's progesterone-induced incubation was not confirmed, the mate's behavior can affect some of the components of nest responsiveness.", "contents": "Role of mate's previous experience in ring dove hormone-induced incubation. Progesterone-induced incubation was examined in 40 pairs of adult ring doves, differing in their previous reproductive experience (courtship vs. nest building) and the previous reproductive experience of their mates (courtship vs. nest building) during testing. Only the previous experience of the experimental subject significantly affected the establishment of incubation during the 24-hr test period. More doves experienced with nest building established incubation than did courtship-experienced doves. The latency to enter the nest area and the latency to stand on the nest were affected by both the previous breeding experience of the subject and the previous breeding experience of its mate. Nest-building-experienced mates decreased the latencies of courtship-experienced subjects, and courtship-experienced mates increased the latencies of nest-building-experienced doves. Males were not more affected by their mates' experience than were females. Time spent on the nest was affected only by the subject's previous experience and sex. While the hypothesis that the behavior of the mate can affect a dove's progesterone-induced incubation was not confirmed, the mate's behavior can affect some of the components of nest responsiveness.", "PMID": 977821}
{"id": "pubmed23n0032_11377", "title": "Hormonal control of female sexual attractiveness proceptivity, and receptivity in rhesus monkeys.", "content": "The sexual behavior of 18 female and 11 male rhesus monkeys was observed in pair tests, in two experiments. In one experiment the females controlled the occurrence of pair tests. The females were ovariectomized and treated with estradiol, two dosages of testosterone, estradiol plus testosterone, estradiol plus dexamethasone, or no hormone; and the effects of the treatment on female attractiveness, proceptivity, and receptivity were examined. Sexual attractiveness of females to males (the effectiveness of the female as a sexual stimulus) was affected by estrogen and androgen. Estrogen enhanced attractiveness, but the effect of androgen depended on the dose. Female sexual proceptivity (the extent to which the female seeks out the male and elicits sexual behavior) was heightened by estrogen and androgen. Female sexual receptivity (willingness to receive a male in copulation was somewhat stimulated by estradiol, but not by testosterone. Both estradiol and testosterone had a greater effect on attractiveness and proceptivity than on receptivity. The hypothesis that testosterone is the \"libidinal hormone\" in female primates was not supported.", "contents": "Hormonal control of female sexual attractiveness proceptivity, and receptivity in rhesus monkeys. The sexual behavior of 18 female and 11 male rhesus monkeys was observed in pair tests, in two experiments. In one experiment the females controlled the occurrence of pair tests. The females were ovariectomized and treated with estradiol, two dosages of testosterone, estradiol plus testosterone, estradiol plus dexamethasone, or no hormone; and the effects of the treatment on female attractiveness, proceptivity, and receptivity were examined. Sexual attractiveness of females to males (the effectiveness of the female as a sexual stimulus) was affected by estrogen and androgen. Estrogen enhanced attractiveness, but the effect of androgen depended on the dose. Female sexual proceptivity (the extent to which the female seeks out the male and elicits sexual behavior) was heightened by estrogen and androgen. Female sexual receptivity (willingness to receive a male in copulation was somewhat stimulated by estradiol, but not by testosterone. Both estradiol and testosterone had a greater effect on attractiveness and proceptivity than on receptivity. The hypothesis that testosterone is the \"libidinal hormone\" in female primates was not supported.", "PMID": 977822}
{"id": "pubmed23n0032_11378", "title": "Ventromedial hypothalamus and short-term feeding suppression by caerulein in male rats.", "content": "A series of six experiments examined the neural loci responsible for caerulein's suppression of eating. Caerulein is a decapeptide chemically and physiologically similar to cholecystokinin, a naturally occurring gut hormone in rats. Rats with lesions in the ventromedial hypothalamus (VMH) showed reduced sensitivity to caerulein (1 mug/kg); rats with lateral hypothalamic (LH) destruction showed heightened sensitivity. Microinjections of caerulein into the VMH, but not into the LH, limited feeding. Finally, tritiated caerulein was selectively bound to tissue in the VMH. The results are discussed in terms of the hypothesis that the VMH manages postprandial inhibition in the rat.", "contents": "Ventromedial hypothalamus and short-term feeding suppression by caerulein in male rats. A series of six experiments examined the neural loci responsible for caerulein's suppression of eating. Caerulein is a decapeptide chemically and physiologically similar to cholecystokinin, a naturally occurring gut hormone in rats. Rats with lesions in the ventromedial hypothalamus (VMH) showed reduced sensitivity to caerulein (1 mug/kg); rats with lateral hypothalamic (LH) destruction showed heightened sensitivity. Microinjections of caerulein into the VMH, but not into the LH, limited feeding. Finally, tritiated caerulein was selectively bound to tissue in the VMH. The results are discussed in terms of the hypothesis that the VMH manages postprandial inhibition in the rat.", "PMID": 977823}
{"id": "pubmed23n0032_11379", "title": "Molecular weights of three mouse milk caseins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and kappa-like characteristics of a fourth casein.", "content": "Caseins of mouse milk are phosphoproteins which precipitate at pH 4.6, stain blue with \"Stains-all,\" and stain red with \"Stains-all\" following alkaline phosphatase digestion. Four caseins were separated electrophoretically in sodium dodecyl sulfate-polyacrylamide gels varying from 8.5 to 15% acrylamide. Molecular weights for three of these proteins were 43,200, 27,700, and 25,900. The molecular weights determined for bovine alphas1 and beta caseins by this method were similar to those previously obtained by other methods. A fourth mouse casein contained carbohydrate, phosphorus, and sialic acid. This protein was rennin-sensitive and behaved anomalously on sodium dodecyl sulfate polyacrylamide gels, as did bovine kappa-casein. Because of similarities with bovine kappa-casein, it was designated with \"kappa-casein\" of mouse milk.", "contents": "Molecular weights of three mouse milk caseins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and kappa-like characteristics of a fourth casein. Caseins of mouse milk are phosphoproteins which precipitate at pH 4.6, stain blue with \"Stains-all,\" and stain red with \"Stains-all\" following alkaline phosphatase digestion. Four caseins were separated electrophoretically in sodium dodecyl sulfate-polyacrylamide gels varying from 8.5 to 15% acrylamide. Molecular weights for three of these proteins were 43,200, 27,700, and 25,900. The molecular weights determined for bovine alphas1 and beta caseins by this method were similar to those previously obtained by other methods. A fourth mouse casein contained carbohydrate, phosphorus, and sialic acid. This protein was rennin-sensitive and behaved anomalously on sodium dodecyl sulfate polyacrylamide gels, as did bovine kappa-casein. Because of similarities with bovine kappa-casein, it was designated with \"kappa-casein\" of mouse milk.", "PMID": 977824}
{"id": "pubmed23n0032_11380", "title": "Optimum amino acid complement for protein synthesis by rat mammary cells in tissue culture.", "content": "The amino acid requirement of rat mammary cells for milk protein synthesis was investigated in dispersed cell culture. A three-dimensional central composite design utilizing three variables (X1 = lysine; X2= methionine, valine, and arginine; X3 = isoleucine, tryptophan, threonine, phenylalanine, and histidine) at five concentrations each, was duplicated twice with mammary cells from lactating Sprague-Dawley rats. The optimum combination of amino acids for maximum milk protein synthesis from multiple regression models was X1 15.0-, X2 4.5-, and X3 1.5-fold their quantities in Eagle's minimal essential medium with leucine, tyrosine, cystine, and glutamine at the base 1-fold in the medium.", "contents": "Optimum amino acid complement for protein synthesis by rat mammary cells in tissue culture. The amino acid requirement of rat mammary cells for milk protein synthesis was investigated in dispersed cell culture. A three-dimensional central composite design utilizing three variables (X1 = lysine; X2= methionine, valine, and arginine; X3 = isoleucine, tryptophan, threonine, phenylalanine, and histidine) at five concentrations each, was duplicated twice with mammary cells from lactating Sprague-Dawley rats. The optimum combination of amino acids for maximum milk protein synthesis from multiple regression models was X1 15.0-, X2 4.5-, and X3 1.5-fold their quantities in Eagle's minimal essential medium with leucine, tyrosine, cystine, and glutamine at the base 1-fold in the medium.", "PMID": 977825}
{"id": "pubmed23n0032_11381", "title": "Effects of zinc, copper, and manganese supplementation of high-concentrate ration on gastrointestinal absorption of copper and manganese in Holstein calves.", "content": "Sixteen Holstein bull calves were fed a high-concentrate ratio supplemented with zinc, copper, and manganese, alone or in combination, for 10 wk. After 6 days of preliminary feeding of chromic oxide, fecal grab-samples were collected for 6 days. Calves were slaughtered, and samples of the contents of the reticulo-rumen, omasum, abomasum, small intestine, cecum, and large intestine were analyzed for chromic oxide, copper, and manganese. Absorption along the gastrointestinal tract, measured by comparison of one segment with the previous segment, indicated net secretion of copper in the abomasum but net absorption from the rest of the tract. The exception was that net secretion into the reticulo-rumen was associated with low dietary copper. Net secretion of manganese was mainly in the small intestine and cecum in all treatments. A higher proportion of copper apparently was absorbed when this mineral was supplemented in the ration. However, absorption of manganese was decreased slightly when manganese was supplemented in the ration. Reabsorption of manganese in the large intestine was probably greater than that of copper.", "contents": "Effects of zinc, copper, and manganese supplementation of high-concentrate ration on gastrointestinal absorption of copper and manganese in Holstein calves. Sixteen Holstein bull calves were fed a high-concentrate ratio supplemented with zinc, copper, and manganese, alone or in combination, for 10 wk. After 6 days of preliminary feeding of chromic oxide, fecal grab-samples were collected for 6 days. Calves were slaughtered, and samples of the contents of the reticulo-rumen, omasum, abomasum, small intestine, cecum, and large intestine were analyzed for chromic oxide, copper, and manganese. Absorption along the gastrointestinal tract, measured by comparison of one segment with the previous segment, indicated net secretion of copper in the abomasum but net absorption from the rest of the tract. The exception was that net secretion into the reticulo-rumen was associated with low dietary copper. Net secretion of manganese was mainly in the small intestine and cecum in all treatments. A higher proportion of copper apparently was absorbed when this mineral was supplemented in the ration. However, absorption of manganese was decreased slightly when manganese was supplemented in the ration. Reabsorption of manganese in the large intestine was probably greater than that of copper.", "PMID": 977826}
{"id": "pubmed23n0032_11382", "title": "Fatty acids of vegetative cells and spores of Bacillus licheniformis.", "content": "Lipids were extracted from vegetative cells and spores of Bacillus licheniformis. Vegetative cells were grown in nutrient broth and spores on nutrient agar. Total lipid approximated 2.89% of the dry weight of vegetative cells and 2.09% of the dry weight of spores. The fatty acids were prepared as methyl esters and analyzed by gas chromatography and mass spectrometry. There were six fatty acids in concentrations greater than 5% of the total lipid in both spores and vegetative cells, but only palmitic acid was common to both. Fatty acids from vegetative cells in quantities of 5% or more of the total lipid material were lauric, myristic, palmitic, palmitoleic, and linoleic acids. Fatty acids from spores in concentrations greater than 5% of the total lipid were isopentadecylic, palmitic, Carbon-17 iso, and three other long or branched chain fatty acids which were not identified. Spores contained more long and branched chain fatty acids with odd numbers of carbon atoms than did vegetative cells.", "contents": "Fatty acids of vegetative cells and spores of Bacillus licheniformis. Lipids were extracted from vegetative cells and spores of Bacillus licheniformis. Vegetative cells were grown in nutrient broth and spores on nutrient agar. Total lipid approximated 2.89% of the dry weight of vegetative cells and 2.09% of the dry weight of spores. The fatty acids were prepared as methyl esters and analyzed by gas chromatography and mass spectrometry. There were six fatty acids in concentrations greater than 5% of the total lipid in both spores and vegetative cells, but only palmitic acid was common to both. Fatty acids from vegetative cells in quantities of 5% or more of the total lipid material were lauric, myristic, palmitic, palmitoleic, and linoleic acids. Fatty acids from spores in concentrations greater than 5% of the total lipid were isopentadecylic, palmitic, Carbon-17 iso, and three other long or branched chain fatty acids which were not identified. Spores contained more long and branched chain fatty acids with odd numbers of carbon atoms than did vegetative cells.", "PMID": 977827}
{"id": "pubmed23n0032_11383", "title": "Hydroxyproline in serum as a homeostatic index for calcium in cattle.", "content": "Hydroxyproline, calcium, magnesium, phosphorus, and alkaline phosphatase values were determined in serum over 24 h in Holstein cows. The cows represented two age groups and three percents of diet calcium. Hydroxyproline followed a cyclic pattern dipping at 0800 and 1600 h after milking at 0500 and 1530 h. Phosphorus showed a 24 h rhythm peaking at 1600 h. No other time effects were demonstrated. Hydroxyproline and alkaline phosphatase were both lower in the older cows, indicating a decreased calcium mobilization from bone with age. There was a correlation coefficient of only .20 between serum calcium and hydroxyproline. The calcium concentration in serum was maintained within a narrow range presumably as a result of homeostatic mechanisms involving bone resorption, i.e. the release of calcium and hydroxyproline may indicate the degree of homeostasis required to maintain serum calcium.", "contents": "Hydroxyproline in serum as a homeostatic index for calcium in cattle. Hydroxyproline, calcium, magnesium, phosphorus, and alkaline phosphatase values were determined in serum over 24 h in Holstein cows. The cows represented two age groups and three percents of diet calcium. Hydroxyproline followed a cyclic pattern dipping at 0800 and 1600 h after milking at 0500 and 1530 h. Phosphorus showed a 24 h rhythm peaking at 1600 h. No other time effects were demonstrated. Hydroxyproline and alkaline phosphatase were both lower in the older cows, indicating a decreased calcium mobilization from bone with age. There was a correlation coefficient of only .20 between serum calcium and hydroxyproline. The calcium concentration in serum was maintained within a narrow range presumably as a result of homeostatic mechanisms involving bone resorption, i.e. the release of calcium and hydroxyproline may indicate the degree of homeostasis required to maintain serum calcium.", "PMID": 977828}
{"id": "pubmed23n0032_11384", "title": "Peripheral plasma determinations of prostaglandin E in asthmatics.", "content": "Prostaglandins, unsaturated fatty acid derivatives with diversified pharmacologic activity, have recently been implicated in the pathophysiology of reversible airway disease. This study attempted to elucidate baseline prostaglandin E (PGE) plasma levels in asthmatics and the change in these levels after stimulation by a beta-adrenergic agent. Fourteen stable, ambulant patients with reversible airway disease and 28 nonatopic control subjects were studied. All had baseline PGE levels performed. The asthmatics were asked to abstain from all medication for 10 hr prior to evaluation. After baseline evaluation consisting of plasma PGE determination, pulmonary function test, blood pressure, and heart rate, 375 mug of aerosolized terbutaline sulfate was administered to 8 of the asthmatics plus 4 of the nonatopic control subjects; the above measurements were repeated at 15, 30, and 60 min after administration. There was a statistically significant difference in baseline plasma PGE levels between the asthmatics (PGE = 432 +/- 81) and the nonatopic control subjects (PGE = 89 +/- 9) (p less than 0.002). Following terbutaline administration, there was no significant change observed in PGE levels in asthmatic or in control individuals (asthmatics: 0 min, 570, 15 min, 513, 30 min, 514, 60 min, 608; normal subjects: 0 min, 138, 15 min, 137, 30 min, 143, 60 min, 214). In summary, we observed a significant difference in baseline PGE levels between asthmatic and nonatopic control persons. No change, however, was noted in PGE levels after beta-adrenergic receptor stimulation. This observation is consistent with the current hypothesis that beta-adrenergic agents act independently of prostaglandins to increase adenyl cyclase and modify bronchiole smooth muscle tone.", "contents": "Peripheral plasma determinations of prostaglandin E in asthmatics. Prostaglandins, unsaturated fatty acid derivatives with diversified pharmacologic activity, have recently been implicated in the pathophysiology of reversible airway disease. This study attempted to elucidate baseline prostaglandin E (PGE) plasma levels in asthmatics and the change in these levels after stimulation by a beta-adrenergic agent. Fourteen stable, ambulant patients with reversible airway disease and 28 nonatopic control subjects were studied. All had baseline PGE levels performed. The asthmatics were asked to abstain from all medication for 10 hr prior to evaluation. After baseline evaluation consisting of plasma PGE determination, pulmonary function test, blood pressure, and heart rate, 375 mug of aerosolized terbutaline sulfate was administered to 8 of the asthmatics plus 4 of the nonatopic control subjects; the above measurements were repeated at 15, 30, and 60 min after administration. There was a statistically significant difference in baseline plasma PGE levels between the asthmatics (PGE = 432 +/- 81) and the nonatopic control subjects (PGE = 89 +/- 9) (p less than 0.002). Following terbutaline administration, there was no significant change observed in PGE levels in asthmatic or in control individuals (asthmatics: 0 min, 570, 15 min, 513, 30 min, 514, 60 min, 608; normal subjects: 0 min, 138, 15 min, 137, 30 min, 143, 60 min, 214). In summary, we observed a significant difference in baseline PGE levels between asthmatic and nonatopic control persons. No change, however, was noted in PGE levels after beta-adrenergic receptor stimulation. This observation is consistent with the current hypothesis that beta-adrenergic agents act independently of prostaglandins to increase adenyl cyclase and modify bronchiole smooth muscle tone.", "PMID": 977861}
{"id": "pubmed23n0032_11385", "title": "Allergens in bee venom. I. Separation and identification of the major allergens.", "content": "Honeybee venom was separated into seven fractions by gel filtration on Sephadex G-75. Allergenic activities of these fractions were assessed by the paper disc radioallergosorbent test (RAST) with a panel of sera from 24 individuals who had systemic reactions to bee stings, 7 who had large local reactions, and 10 control subjects who had reactions of 5 cm or less following bee stings. Three fractions were identified by enzyme or direct hemolytic activity. Twenty-nine of 31 sera from patients having either systemic or large local reactions to bee stings were positive when radioallergosorbent tested with whole bee venom; 22 were positive to phospholipase A, and 28 were positive to both fractions 1 and 2. Thirteen sera combined most strongly with fraction 1, 12 sera most strongly with fraction 2, hyaluronidase, and three sera about equally with fractions 1, 2, and 3. Reactions with other fractions were much weaker. Fractions 1 and 2 were potent inhibitors of RAST with whole venom in the sera reacting most strongly with fractions 1 or 2, respectively. Fraction 3, phospholipase A, and commercial bee venom phospholipase A were significantly less potent inhibitors with the sera tested. In the cases in which IgE antibody bindings to fractions 1, 2, and 3 were of similar magnitude, inhibitions of RAST using the various fractions both on the discs and as inhibitors demonstrated substantial cross-reactivity between the fractions. These results strongly indicate that by using RAST with human sera from bee sting-sensitive individuals, fraction 1, the high molecular materials, and fraction 2, hyaluronidase, are the major allergens in honeybee venom. Phospholipase A appears to be of secondary importance.", "contents": "Allergens in bee venom. I. Separation and identification of the major allergens. Honeybee venom was separated into seven fractions by gel filtration on Sephadex G-75. Allergenic activities of these fractions were assessed by the paper disc radioallergosorbent test (RAST) with a panel of sera from 24 individuals who had systemic reactions to bee stings, 7 who had large local reactions, and 10 control subjects who had reactions of 5 cm or less following bee stings. Three fractions were identified by enzyme or direct hemolytic activity. Twenty-nine of 31 sera from patients having either systemic or large local reactions to bee stings were positive when radioallergosorbent tested with whole bee venom; 22 were positive to phospholipase A, and 28 were positive to both fractions 1 and 2. Thirteen sera combined most strongly with fraction 1, 12 sera most strongly with fraction 2, hyaluronidase, and three sera about equally with fractions 1, 2, and 3. Reactions with other fractions were much weaker. Fractions 1 and 2 were potent inhibitors of RAST with whole venom in the sera reacting most strongly with fractions 1 or 2, respectively. Fraction 3, phospholipase A, and commercial bee venom phospholipase A were significantly less potent inhibitors with the sera tested. In the cases in which IgE antibody bindings to fractions 1, 2, and 3 were of similar magnitude, inhibitions of RAST using the various fractions both on the discs and as inhibitors demonstrated substantial cross-reactivity between the fractions. These results strongly indicate that by using RAST with human sera from bee sting-sensitive individuals, fraction 1, the high molecular materials, and fraction 2, hyaluronidase, are the major allergens in honeybee venom. Phospholipase A appears to be of secondary importance.", "PMID": 977862}
{"id": "pubmed23n0032_11386", "title": "The effects of topical saline and isoproterenol on nasal airway resistance.", "content": "Following determination of baseline nasal airway resistance (NAR) in human subjects, phosphate-buffered saline (PBS) was aerosolized into each nostril and NAR measurements repeated 8 times in 15 minutes. The mean maximal NAR increase in 102 subjects was 22.5% (SD +/- 24.5%) with no significant difference between atopic and nonatopic persons. Hay fever patients did not show significantly increased responsiveness to PBS while symptomatic. During 242 repeat challenges with PBS in 67 asymptomatic subjects, NAR increased over 50% in 24 tests. Following a series of 6 PBS challenges at 15-minute intervals, 10 of 71 subjects had more than a 100% increase in NAR over initial baseline values. Administration of PBS on cotton pledgets, pipetting PBS into the nose, or even just inserting a nasal speculum produced greater increases in NAR than the usual aerosol method. Intranasal atropine effectively blocked PBS-induced increases in NAR, suggesting parasympathetic stimulation as a mechanism. Isoproterenol increased NAR over 100% in 27 of 53 subjects, with no significant difference among rhinitic, asthmatic, and nonatopic individuals. This effect was inhibited by propranolol. Isoproterenol administration to 12 ragweed-sensitive subjects 15 minutes prior to ragweed challenge produced a variable inhibition in NAR responses or no protection at all. Thus the direct effects of isoproterenol on the nasal vasculature tend to outweigh those expected from inhibition of mediator release under the conditions of this study.", "contents": "The effects of topical saline and isoproterenol on nasal airway resistance. Following determination of baseline nasal airway resistance (NAR) in human subjects, phosphate-buffered saline (PBS) was aerosolized into each nostril and NAR measurements repeated 8 times in 15 minutes. The mean maximal NAR increase in 102 subjects was 22.5% (SD +/- 24.5%) with no significant difference between atopic and nonatopic persons. Hay fever patients did not show significantly increased responsiveness to PBS while symptomatic. During 242 repeat challenges with PBS in 67 asymptomatic subjects, NAR increased over 50% in 24 tests. Following a series of 6 PBS challenges at 15-minute intervals, 10 of 71 subjects had more than a 100% increase in NAR over initial baseline values. Administration of PBS on cotton pledgets, pipetting PBS into the nose, or even just inserting a nasal speculum produced greater increases in NAR than the usual aerosol method. Intranasal atropine effectively blocked PBS-induced increases in NAR, suggesting parasympathetic stimulation as a mechanism. Isoproterenol increased NAR over 100% in 27 of 53 subjects, with no significant difference among rhinitic, asthmatic, and nonatopic individuals. This effect was inhibited by propranolol. Isoproterenol administration to 12 ragweed-sensitive subjects 15 minutes prior to ragweed challenge produced a variable inhibition in NAR responses or no protection at all. Thus the direct effects of isoproterenol on the nasal vasculature tend to outweigh those expected from inhibition of mediator release under the conditions of this study.", "PMID": 977863}
{"id": "pubmed23n0032_11387", "title": "Evaluation of exercise-induced bronchospasm in the adult asthmatic.", "content": "It has been shown that most asthmatics respond to exercise with bronchospasm. This study was undertaken to develop a safe and reliable method for quantifying exercise-induced bronchospasm in the asthmatic adult. Five normal adult volunteers and 12 stable asthmatics were exercised to 80% of their predicted maximal heart rate according to a multistage branching treadmill protocol. Their responses in terms of forced expiratory volume in 1 sec (FEV1) and maximum midexpriatory flow rate (MMEFR) at 5, 15, and 30 min after exercise were assessed, while standing, with a Jones Pulmonar II waterless spirometer. This submaximal stress test was chosen because 80% of predicted maximal heart rate could be obtained by all individuals and allowed the asthmatics to exercise long enough for inducible bronchospasm to occur. Audible wheezing was induced in 100% of the asthmatics and in none of the nonasthmatics. In the normal individuals, FEV1 and MMEFR increased signficantly during the postexercise period. When compared to normal subjects, the 12 asthmatic patients demonstrated a significant reduction in FEV1 an MMEFR (deltaFEV1 : 5 min, -300; 15 min, -304; o9 min, -208 ml; -18%, -17%, and -15%; deltaMMEFR: 5 min, -15; 15 min, -9; 30 min, -1L/M; -23%, -18%, and -6%) (p less than 0.01). The use of a rigidly controlled exercise stress with a cardiovascular endpoint in the measurement of FEV1 and MMEFR in the postexercise period appears to be a useful tool in assessing the presence and severity of exercise-induced bronchospasm in the adult asthmatic.", "contents": "Evaluation of exercise-induced bronchospasm in the adult asthmatic. It has been shown that most asthmatics respond to exercise with bronchospasm. This study was undertaken to develop a safe and reliable method for quantifying exercise-induced bronchospasm in the asthmatic adult. Five normal adult volunteers and 12 stable asthmatics were exercised to 80% of their predicted maximal heart rate according to a multistage branching treadmill protocol. Their responses in terms of forced expiratory volume in 1 sec (FEV1) and maximum midexpriatory flow rate (MMEFR) at 5, 15, and 30 min after exercise were assessed, while standing, with a Jones Pulmonar II waterless spirometer. This submaximal stress test was chosen because 80% of predicted maximal heart rate could be obtained by all individuals and allowed the asthmatics to exercise long enough for inducible bronchospasm to occur. Audible wheezing was induced in 100% of the asthmatics and in none of the nonasthmatics. In the normal individuals, FEV1 and MMEFR increased signficantly during the postexercise period. When compared to normal subjects, the 12 asthmatic patients demonstrated a significant reduction in FEV1 an MMEFR (deltaFEV1 : 5 min, -300; 15 min, -304; o9 min, -208 ml; -18%, -17%, and -15%; deltaMMEFR: 5 min, -15; 15 min, -9; 30 min, -1L/M; -23%, -18%, and -6%) (p less than 0.01). The use of a rigidly controlled exercise stress with a cardiovascular endpoint in the measurement of FEV1 and MMEFR in the postexercise period appears to be a useful tool in assessing the presence and severity of exercise-induced bronchospasm in the adult asthmatic.", "PMID": 977864}
{"id": "pubmed23n0032_11388", "title": "Measurement of plasma prostaglandins during exercise-induced bronchospasm.", "content": "Bronchospasm following exercise is a phenomenon which occurs in most patients with reversible airway disease. The pathophysiologic mechanism leading to this bronchoconstriction with exercise has not yet been defined. Recently, prostaglandins have been implicated in the etiology of asthma. The purpose of this investigation was to determine changes in plasma prostaglandins occurring during exercise-induced asthma. Eight ambulant asthmatics were chosen for baseline pulmonary spirometry and peripheral venous blood prostaglandin E and F levels. The asthmatics were then exercised to 80% of their age-predicted maximal heart rate via a multistage branching treadmill protocol. At 5, 15, and 30 min following exercise, pulmonary spirometry was again performed and peripheral venous blood collected at the indicated times. Clinical bronchospasm as characterized by audible wheezing and greater than 15% decrease in FEV1 and MMEFR was obtained in all of the asthmatics. Peripheral PGE and PGF2alpha determinations following this exercise protocol were not altered significantly: (PGE: 0 min, 238; 5 min, 185; 15 min, 248; 30 min, 256 pg/ml); (PGF2alpha: 0 min, 50; 5 min, 24; 15 min, 25; 30 min, 17 pg/ml) (p less than 0.1). In summary, no significant change in peripheral blood prostaglandin E and F2alpha levels as determined by radioimmunoassay was noted at the time of exercise-induced bronchospasm.", "contents": "Measurement of plasma prostaglandins during exercise-induced bronchospasm. Bronchospasm following exercise is a phenomenon which occurs in most patients with reversible airway disease. The pathophysiologic mechanism leading to this bronchoconstriction with exercise has not yet been defined. Recently, prostaglandins have been implicated in the etiology of asthma. The purpose of this investigation was to determine changes in plasma prostaglandins occurring during exercise-induced asthma. Eight ambulant asthmatics were chosen for baseline pulmonary spirometry and peripheral venous blood prostaglandin E and F levels. The asthmatics were then exercised to 80% of their age-predicted maximal heart rate via a multistage branching treadmill protocol. At 5, 15, and 30 min following exercise, pulmonary spirometry was again performed and peripheral venous blood collected at the indicated times. Clinical bronchospasm as characterized by audible wheezing and greater than 15% decrease in FEV1 and MMEFR was obtained in all of the asthmatics. Peripheral PGE and PGF2alpha determinations following this exercise protocol were not altered significantly: (PGE: 0 min, 238; 5 min, 185; 15 min, 248; 30 min, 256 pg/ml); (PGF2alpha: 0 min, 50; 5 min, 24; 15 min, 25; 30 min, 17 pg/ml) (p less than 0.1). In summary, no significant change in peripheral blood prostaglandin E and F2alpha levels as determined by radioimmunoassay was noted at the time of exercise-induced bronchospasm.", "PMID": 977865}
{"id": "pubmed23n0032_11389", "title": "Dietary interviewing by computer. An experimental approach to counseling.", "content": "A computer-based dietary counseling system was tested by sixty-four volunteers from the Boston area. Most of the patients seemed to benefit from their interaction with the computer, and the two participating nutritionists frequently found that the program was useful as an antecedent to their own one-to-one sessions with the patients.", "contents": "Dietary interviewing by computer. An experimental approach to counseling. A computer-based dietary counseling system was tested by sixty-four volunteers from the Boston area. Most of the patients seemed to benefit from their interaction with the computer, and the two participating nutritionists frequently found that the program was useful as an antecedent to their own one-to-one sessions with the patients.", "PMID": 977878}
{"id": "pubmed23n0032_11390", "title": "Comprehensive evaluation of fatty acids in foods. IX. Fowl.", "content": "An extensive survey of the post-1960 literature on the lipids of fowl has been completed. This review shows that the total lipid content of chicken tissues increases with age. The fatty acid composition of both chicken and turkey tissues reflects the fatty acid composition of the dietary fat. The fatty acid composition data for dark meat, light meat, or skin of all classes of chicken --broiler-fryer, roaster, and stewing hen--raised on diets equivalent to commercial feed were combined into single tissue-type fatty acid profiles. Similar profiles were also established for roaster-fryer turkey, young tom turkey, and young hen turkey. Breed, sex, and environmental temperature showed only minor effects on the tissue fatty acid compositions. Young chicken flesh contains less fat than that of stewing hens, turkeys, and other fowl, which all have approximately equal total fat content. White meat of chicken and turkey contains the least amount of fat, and the skin of all birds contained the largest amount, duck and goose skin containing the greatest of all. Tables of the total fat and fatty acid composition of tissue and tissue composites of fowl are given. Studies on chicken and turkey tissues were sufficiently numerous that the computed fatty acid compositions in the tables are considered reliable representation of market birds. Data for goose, duck, partridge, pheasant, pigeon, and quail were computed from sparse information and should be viewedas provisional guides.", "contents": "Comprehensive evaluation of fatty acids in foods. IX. Fowl. An extensive survey of the post-1960 literature on the lipids of fowl has been completed. This review shows that the total lipid content of chicken tissues increases with age. The fatty acid composition of both chicken and turkey tissues reflects the fatty acid composition of the dietary fat. The fatty acid composition data for dark meat, light meat, or skin of all classes of chicken --broiler-fryer, roaster, and stewing hen--raised on diets equivalent to commercial feed were combined into single tissue-type fatty acid profiles. Similar profiles were also established for roaster-fryer turkey, young tom turkey, and young hen turkey. Breed, sex, and environmental temperature showed only minor effects on the tissue fatty acid compositions. Young chicken flesh contains less fat than that of stewing hens, turkeys, and other fowl, which all have approximately equal total fat content. White meat of chicken and turkey contains the least amount of fat, and the skin of all birds contained the largest amount, duck and goose skin containing the greatest of all. Tables of the total fat and fatty acid composition of tissue and tissue composites of fowl are given. Studies on chicken and turkey tissues were sufficiently numerous that the computed fatty acid compositions in the tables are considered reliable representation of market birds. Data for goose, duck, partridge, pheasant, pigeon, and quail were computed from sparse information and should be viewedas provisional guides.", "PMID": 977879}
{"id": "pubmed23n0032_11391", "title": "Fate of DDT and parathion in grapes processed into arak, an alcoholic beverage.", "content": "Arak, the national alcoholic drink in Lebanon, was prepared from grapes to which either DDT or parathion had been added. Samples of the nine fractions produced from the fermentation and distillation steps were analyzed for DDT and parathion and their respective metabolites. DDT degraded to DDD during the fermentation step resulting in a sharp decrease in DDT level. The two distillation steps contributed to a further decrease in the DDT level so that the final product contained less than 2% of the amount found in the fresh grape juice. Although the concentration of DDD increased sharply during fermentation, it also decreased to a negligible level during the subsequent distillation procedure. Parathion was more stable than DDT during the fermentation and first distillation steps. However, the second distillation process caused a sharp decline in its level and the Arak contained only about 6% of the residues present in the fresh juice, paranithophenol being the only metabolite detected.", "contents": "Fate of DDT and parathion in grapes processed into arak, an alcoholic beverage. Arak, the national alcoholic drink in Lebanon, was prepared from grapes to which either DDT or parathion had been added. Samples of the nine fractions produced from the fermentation and distillation steps were analyzed for DDT and parathion and their respective metabolites. DDT degraded to DDD during the fermentation step resulting in a sharp decrease in DDT level. The two distillation steps contributed to a further decrease in the DDT level so that the final product contained less than 2% of the amount found in the fresh grape juice. Although the concentration of DDD increased sharply during fermentation, it also decreased to a negligible level during the subsequent distillation procedure. Parathion was more stable than DDT during the fermentation and first distillation steps. However, the second distillation process caused a sharp decline in its level and the Arak contained only about 6% of the residues present in the fresh juice, paranithophenol being the only metabolite detected.", "PMID": 977917}
{"id": "pubmed23n0032_11392", "title": "Contributions to ecological chemistry CXII1. Balance of conversion of buturon-14C in wheat under outdoor conditions.", "content": "The urea herbicide buturon (N-[p-chlorophenyl]-N'-methyl-N'-isobutinyl-urea), 14C-labeled, was sprayed on winter wheat as an aqueous formulation (2.98 kg/ha) under outdoor conditions. Upon harvest (three months after application), a total of 49.2% of the applied radiocarbon was recovered: 2.0% in the plants, 46.9% in the soil, and 0.3% in the leaching water (depth greater than 50 cm); less than 0.1% was in the grains (0.464 ppm). Only about half of the radioactivity present in plants could be recovered under mild extraction conditions; about half of this was unchanged buturon. In straw and husk extracts, the following metabolites were identified by gaschromatography/mass spectrometry:N-(p-chlorophenyl)-N-methyl-O-methyl-carbamate (metabolite I), N-phenyl-N'-formyl-urea (metabolite II), two unstable metabolites giving (p-chlorophenyl)-isocyanate upon purification (metabolites III and IV), N-(p-chlorophenyl)-N'-methyl-N'-isobutenylol-urea (metabolite V), p-chloroformanilide (metabolite VI) and biologically bound p-chloroaniline (metabolite VII). In the root and basal stem extract, the following metabolites were identified by gas chromatography/mass spectrometry: N-(p-chlorophenyl)-O-methyl-carbamate (metabolite VIII) and N-(p-chlorophenyl)-N'-methyl-urea (metabolite IX).", "contents": "Contributions to ecological chemistry CXII1. Balance of conversion of buturon-14C in wheat under outdoor conditions. The urea herbicide buturon (N-[p-chlorophenyl]-N'-methyl-N'-isobutinyl-urea), 14C-labeled, was sprayed on winter wheat as an aqueous formulation (2.98 kg/ha) under outdoor conditions. Upon harvest (three months after application), a total of 49.2% of the applied radiocarbon was recovered: 2.0% in the plants, 46.9% in the soil, and 0.3% in the leaching water (depth greater than 50 cm); less than 0.1% was in the grains (0.464 ppm). Only about half of the radioactivity present in plants could be recovered under mild extraction conditions; about half of this was unchanged buturon. In straw and husk extracts, the following metabolites were identified by gaschromatography/mass spectrometry:N-(p-chlorophenyl)-N-methyl-O-methyl-carbamate (metabolite I), N-phenyl-N'-formyl-urea (metabolite II), two unstable metabolites giving (p-chlorophenyl)-isocyanate upon purification (metabolites III and IV), N-(p-chlorophenyl)-N'-methyl-N'-isobutenylol-urea (metabolite V), p-chloroformanilide (metabolite VI) and biologically bound p-chloroaniline (metabolite VII). In the root and basal stem extract, the following metabolites were identified by gas chromatography/mass spectrometry: N-(p-chlorophenyl)-O-methyl-carbamate (metabolite VIII) and N-(p-chlorophenyl)-N'-methyl-urea (metabolite IX).", "PMID": 977918}
{"id": "pubmed23n0032_11393", "title": "Movement and metabolism of S-benzyl O,O-diisopropyl phosphorothiolate (Kitazin P) and O-ethyl S,S-diphenyl phosphorodithiolate (edifenphos) in various types of soils.", "content": "Movement and Metabolism of 32P and 35S-double labeled Kitazin P (S-benzyl O,O-diisopropyl phosphorothiolate) and 35S-labeled edifenphos (O-ethyl S,S-diphenyl phosphorodithiolate) were examined with three types of soils, sandy loam, alluvial clay loam, and volcanic ash loam. Vertical movement of both the compounds in soil column was different with soil types, and the order of mobility in soil column was as follows: sandy loam greater than alluvial clay loam greater than volcanic ash loam. Persistence of edifenphos in soil was shorter than that of Kitazin P. Main degradation products at the initial stage of metabolism were S,S,S-triphenyl phosphorotrithiolate, O,O-diethyl S-phenyl phosphorothiolate, S-phenyl dihydrogen phosphorothiolate and diphenyl disulfide in edifenphos and O,O-diisopropyl hydorgen phosphorothioate in Kitazin P. Sulfur atom of Kitazin P was found in sulfuric acid at a minor level through dibenzyl disulfide and toluene-alpha-sulfonic acid, and that of edifenphos was converted to sulfuric acid through diphenyl disulfide and benzenesulfonic acid. Kitazin P under flooded condition of alluvial clay loam was slightly more persistent as compared with upland condition. Sterilized condition of Kitazin P did not cause any appreciable degradation throughout the experimental period, but such condition did not necessarily prevent the degradation of edifenphos.", "contents": "Movement and metabolism of S-benzyl O,O-diisopropyl phosphorothiolate (Kitazin P) and O-ethyl S,S-diphenyl phosphorodithiolate (edifenphos) in various types of soils. Movement and Metabolism of 32P and 35S-double labeled Kitazin P (S-benzyl O,O-diisopropyl phosphorothiolate) and 35S-labeled edifenphos (O-ethyl S,S-diphenyl phosphorodithiolate) were examined with three types of soils, sandy loam, alluvial clay loam, and volcanic ash loam. Vertical movement of both the compounds in soil column was different with soil types, and the order of mobility in soil column was as follows: sandy loam greater than alluvial clay loam greater than volcanic ash loam. Persistence of edifenphos in soil was shorter than that of Kitazin P. Main degradation products at the initial stage of metabolism were S,S,S-triphenyl phosphorotrithiolate, O,O-diethyl S-phenyl phosphorothiolate, S-phenyl dihydrogen phosphorothiolate and diphenyl disulfide in edifenphos and O,O-diisopropyl hydorgen phosphorothioate in Kitazin P. Sulfur atom of Kitazin P was found in sulfuric acid at a minor level through dibenzyl disulfide and toluene-alpha-sulfonic acid, and that of edifenphos was converted to sulfuric acid through diphenyl disulfide and benzenesulfonic acid. Kitazin P under flooded condition of alluvial clay loam was slightly more persistent as compared with upland condition. Sterilized condition of Kitazin P did not cause any appreciable degradation throughout the experimental period, but such condition did not necessarily prevent the degradation of edifenphos.", "PMID": 977919}
{"id": "pubmed23n0032_11394", "title": "Variations in fluorescence characteristics of intact human crystalline lens segments as a function of age.", "content": "Fluorescence spectra of human lenses were examined as a function of age. The relative intensity in the blue-green area was red shifted with age. Absorption and scatter produce a substantial contribution to this shift. Some change, however, was the result of a true fluorescence intensity shift. In the lower blue region a true independent red shift was found in the nucleus, but not in the cortex. In the upper blue region, true red shift in the nucleus was not significant with fluorescence excited at 400 nm but was significant with fluorescence excited at 375 nm; the shift was well defined in the cortex. This pattern is consistent with age-related accumulation of multiple fluorogens or fluorogen precursors in the lens with relative concentration differences in nucleus and cortex.", "contents": "Variations in fluorescence characteristics of intact human crystalline lens segments as a function of age. Fluorescence spectra of human lenses were examined as a function of age. The relative intensity in the blue-green area was red shifted with age. Absorption and scatter produce a substantial contribution to this shift. Some change, however, was the result of a true fluorescence intensity shift. In the lower blue region a true independent red shift was found in the nucleus, but not in the cortex. In the upper blue region, true red shift in the nucleus was not significant with fluorescence excited at 400 nm but was significant with fluorescence excited at 375 nm; the shift was well defined in the cortex. This pattern is consistent with age-related accumulation of multiple fluorogens or fluorogen precursors in the lens with relative concentration differences in nucleus and cortex.", "PMID": 977920}
{"id": "pubmed23n0032_11395", "title": "Gerontological data of C57BL/6J mice. II. Changes in blood cell counts in the course of natural aging.", "content": "A group of 1,052 C578L/6J mice -- 296 males and 756 females - was kept under well defined, clean, conventional conditions from the age of 6 weeks until natural death. At regular time intervals several hematological values were examined from 50 to 60 randomly selected animals of each sex and statistically evaluated. The hematological investigations did not reveal any important sex-related differences. A significant gradual decrease of the RBC counts and hemoglobin content was observed during the life-span. Lymphocyte and total WBC counts were subject to considerable variation. They decreased in the first two-thirds of life and increased in the last third. Neutrophils remained rather stable and exhibited an increasing trend in the higher age. The relative level of lymphocytes decreased signficantly in favour of neutrophils during natural aging.", "contents": "Gerontological data of C57BL/6J mice. II. Changes in blood cell counts in the course of natural aging. A group of 1,052 C578L/6J mice -- 296 males and 756 females - was kept under well defined, clean, conventional conditions from the age of 6 weeks until natural death. At regular time intervals several hematological values were examined from 50 to 60 randomly selected animals of each sex and statistically evaluated. The hematological investigations did not reveal any important sex-related differences. A significant gradual decrease of the RBC counts and hemoglobin content was observed during the life-span. Lymphocyte and total WBC counts were subject to considerable variation. They decreased in the first two-thirds of life and increased in the last third. Neutrophils remained rather stable and exhibited an increasing trend in the higher age. The relative level of lymphocytes decreased signficantly in favour of neutrophils during natural aging.", "PMID": 977921}
{"id": "pubmed23n0032_11396", "title": "A clinical comparison of tests of organicity in elderly patients.", "content": "The quantification and standardization of observations of clinically significant organicity in the elderly is possible through the administration of specific cognitive tests. In this study, four organicity tests were evaluated on a geriatric medical/surgical patient group, with the SPMSQ yielding the best correlation with a clinical diagnosis of organicity. The SPMSQ is also recommended for its ease of administration, brevity, quantitative output, and straight-forward interpretation. The FHT produces an increment of improvement in the identification of organic groups, thus serving as a useful second test. The MSQ, while longer and slightly more complex, is roughly equivalent to the SPMSQ. The MFD test, the most lengthy and complex of the four instruments used, did not add sufficient additional information for this sample to warrant its inclusion in a testing regimen. Accurate diagnosis is critical for the geriatric patient if excessive morbidity and mortality are to be avoided, and unnecessarily long hospital stays are to be reduced. The availability of a short, easily administered test like the SPMSQ can effectively improve the assessment of the elderly patient at little additional cost to the clinician.", "contents": "A clinical comparison of tests of organicity in elderly patients. The quantification and standardization of observations of clinically significant organicity in the elderly is possible through the administration of specific cognitive tests. In this study, four organicity tests were evaluated on a geriatric medical/surgical patient group, with the SPMSQ yielding the best correlation with a clinical diagnosis of organicity. The SPMSQ is also recommended for its ease of administration, brevity, quantitative output, and straight-forward interpretation. The FHT produces an increment of improvement in the identification of organic groups, thus serving as a useful second test. The MSQ, while longer and slightly more complex, is roughly equivalent to the SPMSQ. The MFD test, the most lengthy and complex of the four instruments used, did not add sufficient additional information for this sample to warrant its inclusion in a testing regimen. Accurate diagnosis is critical for the geriatric patient if excessive morbidity and mortality are to be avoided, and unnecessarily long hospital stays are to be reduced. The availability of a short, easily administered test like the SPMSQ can effectively improve the assessment of the elderly patient at little additional cost to the clinician.", "PMID": 977922}
{"id": "pubmed23n0032_11397", "title": "The effects of age and genotype upon the jaw-jerk reflex in inbred mice.", "content": "The threshold of the jaw-jerk reflex to electrical stimulation of the hard palate was measured in a cross-sectional design using C57BL/6J and DBA/2J mice at 2, 8, 20, 25, and 30 mo of age, in order to assess CNS sensitivity as a function of increasing age. The thresholds of DBA/2J mice were higher than those of C57BL/6J mice at all ages tested. No age-related changes in threshold were observed in mice of either strain.", "contents": "The effects of age and genotype upon the jaw-jerk reflex in inbred mice. The threshold of the jaw-jerk reflex to electrical stimulation of the hard palate was measured in a cross-sectional design using C57BL/6J and DBA/2J mice at 2, 8, 20, 25, and 30 mo of age, in order to assess CNS sensitivity as a function of increasing age. The thresholds of DBA/2J mice were higher than those of C57BL/6J mice at all ages tested. No age-related changes in threshold were observed in mice of either strain.", "PMID": 977923}
{"id": "pubmed23n0032_11398", "title": "Relations of age and personality dimensions to cognitive ability factors.", "content": "The relation between three cognitive ability factors - Information Processing Ability (IPA), Manual Dexterity (MD), and Pattern Analysis Capability (PAC) - and three personality dimensions - Anxiety, Extraversion, and Openness to Experience - were examined in three age groups. Subjects were 969 male volunteers ranging in age from 25 to 82. Subjects high in anixety scored lower on all three cognitive factors; subjects open to experience scored higher on IPA and PAC; and introverted subjects scored higher on PAC. Most of these effects remained when the education and socio-economic status were held constant in covariance analyses. Older subjects performed less well than younger ones on MD and PAC, but not on IPA. While personality has some influence on cognitive performance, the declines with age in performance on some cognitive tasks are not mediated by personality.", "contents": "Relations of age and personality dimensions to cognitive ability factors. The relation between three cognitive ability factors - Information Processing Ability (IPA), Manual Dexterity (MD), and Pattern Analysis Capability (PAC) - and three personality dimensions - Anxiety, Extraversion, and Openness to Experience - were examined in three age groups. Subjects were 969 male volunteers ranging in age from 25 to 82. Subjects high in anixety scored lower on all three cognitive factors; subjects open to experience scored higher on IPA and PAC; and introverted subjects scored higher on PAC. Most of these effects remained when the education and socio-economic status were held constant in covariance analyses. Older subjects performed less well than younger ones on MD and PAC, but not on IPA. While personality has some influence on cognitive performance, the declines with age in performance on some cognitive tasks are not mediated by personality.", "PMID": 977924}
{"id": "pubmed23n0032_11399", "title": "Solution shift performance in the elderly.", "content": "Seventy-two elderly adults were presented a concept problem which varied as a function of type of solution shift (reversal or nonreversal) and stimulus materials (compact designs, distributed designs, or distributed foods). Both trials to criterion and postshift error patterns were analyzed. Results indicated that stimulus materials affected postshift performance and that the elderly do respond in a dimensional fashion under some circumstances. Examination of individual protocols revealed that a rigidity notion would not account for the difficulty the elderly experienced when solution was shifted. Several alternative explanations were offered for the failure of many volunteers to solve the postshift problem.", "contents": "Solution shift performance in the elderly. Seventy-two elderly adults were presented a concept problem which varied as a function of type of solution shift (reversal or nonreversal) and stimulus materials (compact designs, distributed designs, or distributed foods). Both trials to criterion and postshift error patterns were analyzed. Results indicated that stimulus materials affected postshift performance and that the elderly do respond in a dimensional fashion under some circumstances. Examination of individual protocols revealed that a rigidity notion would not account for the difficulty the elderly experienced when solution was shifted. Several alternative explanations were offered for the failure of many volunteers to solve the postshift problem.", "PMID": 977925}
{"id": "pubmed23n0032_11400", "title": "Categorization behavior in aging subjects.", "content": "Age changes in categorization and conceptual styles were studied using an object-sorting task in 276 subjects representing 7 age groups: 5, 6, 7, 19-21, 60-69. 70-79, and 80-89. Significant age effects (p less than 01) were found in the analysis of variance for four of six measures; there were no significant sex or interaction effects. Strong quadratic trends were found for number of ungrouped objects, % relational-thematic responses, and % categorical-inferential responses; oldest groups' responses were similar to children's. A linear trend was found for number of groups. There was no significant age effect for category breadth. Use of descriptive-analytic categories by the elderly was negligible compared to younger subjects.", "contents": "Categorization behavior in aging subjects. Age changes in categorization and conceptual styles were studied using an object-sorting task in 276 subjects representing 7 age groups: 5, 6, 7, 19-21, 60-69. 70-79, and 80-89. Significant age effects (p less than 01) were found in the analysis of variance for four of six measures; there were no significant sex or interaction effects. Strong quadratic trends were found for number of ungrouped objects, % relational-thematic responses, and % categorical-inferential responses; oldest groups' responses were similar to children's. A linear trend was found for number of groups. There was no significant age effect for category breadth. Use of descriptive-analytic categories by the elderly was negligible compared to younger subjects.", "PMID": 977926}
{"id": "pubmed23n0032_11401", "title": "Attitudes toward aging as a function of in-service training and practitioner age.", "content": "Age differences in attitudes toward aging were investigated as a function of a 3-hour training program with 322 women (18-74 years) from various geriatric health care and social service contexts. Using a quasi-experimental pre- and posttest design, sites were randomly divided into initial experimental and control groups, with all control participants receiving training following the research program. Results indicated few instances of unfavorable attitudes toward aging in any age group, with younger women evidencing less cynicism toward aging, less social distance from the aged, and slightly less stereotyping. Training appeared to result in less cynicism, stronger endorsements of family and public responsibility, and slightly greater anxiety. No Age X Pretest/Posttest interactions were found. The results are discussed in terms of their implications for service provider-client interaction, the correspondence of attitudinal and behavioral change, and appropriate instruments for assessing program impact.", "contents": "Attitudes toward aging as a function of in-service training and practitioner age. Age differences in attitudes toward aging were investigated as a function of a 3-hour training program with 322 women (18-74 years) from various geriatric health care and social service contexts. Using a quasi-experimental pre- and posttest design, sites were randomly divided into initial experimental and control groups, with all control participants receiving training following the research program. Results indicated few instances of unfavorable attitudes toward aging in any age group, with younger women evidencing less cynicism toward aging, less social distance from the aged, and slightly less stereotyping. Training appeared to result in less cynicism, stronger endorsements of family and public responsibility, and slightly greater anxiety. No Age X Pretest/Posttest interactions were found. The results are discussed in terms of their implications for service provider-client interaction, the correspondence of attitudinal and behavioral change, and appropriate instruments for assessing program impact.", "PMID": 977927}
{"id": "pubmed23n0032_11402", "title": "A re-examination of widowhood and morale.", "content": "Previous research has suggested widowed status to be associated with lower morale or life satisfaction. The effects of marital status on morale relative to five covariates (health, income, age, family interaction, and employment status) were examined with 232 widowed and 363 married women aged 45-74, drawn in a multistage stratified area probability sample of Los Angeles County. Analysis of covariance was utilized with a six-item, factor analyzed measure of morale. Parallel analyses were performed for each of three ethnic groups (blacks, Mexican-American, and white) to examine specific ethnic patterns. In the total sample, poor health was associated with significantly lower morale among widowed than among married women. The analysis with age showed the widowed group catching up and surpassing their married peers on morale at higher ages. Controlling for income and employment status eliminated differences between the marital status groups on morale scores. Higher family interaction was positively associated with morale in both groups, but married women were consistently higher on morale regardless of level of interaction. Most ethnic differences were not significant, but family interaction appeared to be crucial among Mexican-American widows. The findings suggest that lower morale scores found among the widowed may be partly attributable to other factors commonly associated with this status and not due to the role of widowhood per se.", "contents": "A re-examination of widowhood and morale. Previous research has suggested widowed status to be associated with lower morale or life satisfaction. The effects of marital status on morale relative to five covariates (health, income, age, family interaction, and employment status) were examined with 232 widowed and 363 married women aged 45-74, drawn in a multistage stratified area probability sample of Los Angeles County. Analysis of covariance was utilized with a six-item, factor analyzed measure of morale. Parallel analyses were performed for each of three ethnic groups (blacks, Mexican-American, and white) to examine specific ethnic patterns. In the total sample, poor health was associated with significantly lower morale among widowed than among married women. The analysis with age showed the widowed group catching up and surpassing their married peers on morale at higher ages. Controlling for income and employment status eliminated differences between the marital status groups on morale scores. Higher family interaction was positively associated with morale in both groups, but married women were consistently higher on morale regardless of level of interaction. Most ethnic differences were not significant, but family interaction appeared to be crucial among Mexican-American widows. The findings suggest that lower morale scores found among the widowed may be partly attributable to other factors commonly associated with this status and not due to the role of widowhood per se.", "PMID": 977928}
{"id": "pubmed23n0032_11403", "title": "Report from the National Institute on Aging: the research grant support mechanism.", "content": "Most NIH support of research on aging has been in the area of the biomedical sciences, followed by behavioral and social sciences. Research project and program project grants are the primary mechanisms available to the NIA for support of research on aging. The grant process for aging research begins with preparation and review at the applicant institution. The process of review at NIH proceeds from referral to an intitial review group for peer review and NIA for possible funding, NIA staff review, the National Adivisory Council on Aging review, and concludes with NIA funding action. The primary mode of applicant appeal of a negative decision by NIA on an application is reapplication.", "contents": "Report from the National Institute on Aging: the research grant support mechanism. Most NIH support of research on aging has been in the area of the biomedical sciences, followed by behavioral and social sciences. Research project and program project grants are the primary mechanisms available to the NIA for support of research on aging. The grant process for aging research begins with preparation and review at the applicant institution. The process of review at NIH proceeds from referral to an intitial review group for peer review and NIA for possible funding, NIA staff review, the National Adivisory Council on Aging review, and concludes with NIA funding action. The primary mode of applicant appeal of a negative decision by NIA on an application is reapplication.", "PMID": 977929}
{"id": "pubmed23n0032_11404", "title": "[Possible immunologic factors in natural selection of the sexes].", "content": "The foetal graft is protected from immunological rejection by the trophoblastic barrier, antigenically neutral. A deficiency of this protective barrier puts the highly antigenic egg contents in direct contact with the maternal organism. The formation of maternal antibodies, and the rejection reaction of the egg graft due to it, probabally explains certain miscarriages. We think that the male fetus must, in these cases, be more antigenic with regard to the graft carrying maternal organism, than the female fetus. A study of 222 pregnancies, with repeated spontaneous miscarriages confirms the clear preponderance of girls, among the non-premature and well-formed children born, and the efficiency of H.C.G. in protecting threatened pregnancies with male fetus.", "contents": "[Possible immunologic factors in natural selection of the sexes]. The foetal graft is protected from immunological rejection by the trophoblastic barrier, antigenically neutral. A deficiency of this protective barrier puts the highly antigenic egg contents in direct contact with the maternal organism. The formation of maternal antibodies, and the rejection reaction of the egg graft due to it, probabally explains certain miscarriages. We think that the male fetus must, in these cases, be more antigenic with regard to the graft carrying maternal organism, than the female fetus. A study of 222 pregnancies, with repeated spontaneous miscarriages confirms the clear preponderance of girls, among the non-premature and well-formed children born, and the efficiency of H.C.G. in protecting threatened pregnancies with male fetus.", "PMID": 977930}
{"id": "pubmed23n0032_11405", "title": "The cytochemical demonstration of prostatic acid phosphatase using a new substrate, phosphorylcholine.", "content": "Prostatic acid phosphatase (PAP), an acid phosphatase specific to the prostate gland, is demonstrated cytochemically for both light and electron microscopy with a new substrate phosphorylcholine. Lead ion is used as capture agent for liberated phosphate ion in a modified Gomori medium. PAP is demonstrated in the tubuloaveolar epithelial secretory cells of the rat ventral prostate gland. In the apical portion of the cell it is found in secretory granules and in the matrix of multivescular bodies. In the Golgi area it is localized in Golgi cisternae, Golgi related vacuoles and multivescular bodies. Evidence is presented that PAP is not a lysosomal enzyme, as are other acid phosphatases, and that phosphorylcholine is a highly specific substrate for PAP. As based on the role of pentavalent nitrogen on substrate structure, it is apparent that PAP is to other acid phosphatases what the cholinesterases are to other esterases.", "contents": "The cytochemical demonstration of prostatic acid phosphatase using a new substrate, phosphorylcholine. Prostatic acid phosphatase (PAP), an acid phosphatase specific to the prostate gland, is demonstrated cytochemically for both light and electron microscopy with a new substrate phosphorylcholine. Lead ion is used as capture agent for liberated phosphate ion in a modified Gomori medium. PAP is demonstrated in the tubuloaveolar epithelial secretory cells of the rat ventral prostate gland. In the apical portion of the cell it is found in secretory granules and in the matrix of multivescular bodies. In the Golgi area it is localized in Golgi cisternae, Golgi related vacuoles and multivescular bodies. Evidence is presented that PAP is not a lysosomal enzyme, as are other acid phosphatases, and that phosphorylcholine is a highly specific substrate for PAP. As based on the role of pentavalent nitrogen on substrate structure, it is apparent that PAP is to other acid phosphatases what the cholinesterases are to other esterases.", "PMID": 977936}
{"id": "pubmed23n0032_11406", "title": "[Leukocyte reactions \"in vitro\" to human spermatozoa in infertile females].", "content": "Leucocyte migration test was done in 55 couples facing fertility problems for detection of antispermatozoal cellular hypersensitivity. In 29 cases out of 55, the sterility was unexplained and in 26 cases out of 55, various infertility factors were found. In this study, wife's leucocytes separated on Ficoll-Urografin gradient were incubated (4.10(6) per capillary) with washed fresh husband's spermatozoa suspended at 7.10(6)/ml in M 199 and 20 p. 100 fetal bovine serum. The results were read after 18h. Two kinds of migration inhibition were found: 1) Specific wives leucocyte migration inhibition in 4 out of 55 cases, all of them unexplained infertility cases. 2) Leucocyte migration inhibition not related to wife's immunity state and possibly due to a direct effect of a toxic or inhibitory property of some husband's spermatozoa in 22 out of 55 cases, 11/19 cases with known male factors, and 11/36 cases without any male factor. In the follow up, only 1 woman out of the 22 couples with husband's spermatozoal inhibitory factor was pregnant later, while in the group of 29 women in which normal leucocyte spreading was found, 8 women conceived later. No woman out of the 4 suspected of antispermatozoal delayed hypersensitivity have conceived. Attention must be paid to the possible role of delayed hypersensitivity to sperm in unexplained infertility and also to the occurrence of spermatozoal toxic factor and its possible implications on human infertility.", "contents": "[Leukocyte reactions \"in vitro\" to human spermatozoa in infertile females]. Leucocyte migration test was done in 55 couples facing fertility problems for detection of antispermatozoal cellular hypersensitivity. In 29 cases out of 55, the sterility was unexplained and in 26 cases out of 55, various infertility factors were found. In this study, wife's leucocytes separated on Ficoll-Urografin gradient were incubated (4.10(6) per capillary) with washed fresh husband's spermatozoa suspended at 7.10(6)/ml in M 199 and 20 p. 100 fetal bovine serum. The results were read after 18h. Two kinds of migration inhibition were found: 1) Specific wives leucocyte migration inhibition in 4 out of 55 cases, all of them unexplained infertility cases. 2) Leucocyte migration inhibition not related to wife's immunity state and possibly due to a direct effect of a toxic or inhibitory property of some husband's spermatozoa in 22 out of 55 cases, 11/19 cases with known male factors, and 11/36 cases without any male factor. In the follow up, only 1 woman out of the 22 couples with husband's spermatozoal inhibitory factor was pregnant later, while in the group of 29 women in which normal leucocyte spreading was found, 8 women conceived later. No woman out of the 4 suspected of antispermatozoal delayed hypersensitivity have conceived. Attention must be paid to the possible role of delayed hypersensitivity to sperm in unexplained infertility and also to the occurrence of spermatozoal toxic factor and its possible implications on human infertility.", "PMID": 977931}
{"id": "pubmed23n0032_11407", "title": "Variation in the autoradiographic technique: I. Emulsion-developer combinations assessed by photometric measurement of single silver grains.", "content": "Five commonly used developers (Acufine, Rodinal, Dektol, D19 and Microdol X) and two popular nuclear gel emulsions (Kodak NTB-2 and Ilford L4) were tested in combinations for adequacy of development characteristics and the distribution of silver particle (grain) size measured by means of a reflectance microphotometer. All developer-emulsion combinations had some range of development time during which maximum development was accomplished with no occurrence of background grains, with the exceptions of the combinations of NTB2-Acufine and NTB2-D19. Ilford L4 emulsion obtained the narrowest grain size distribution with Rodinal and Acufine, followed by Dektol, and D19. The narrowest size distribution for Kodak NTB2 emulsion was achieved with D19 followed by Acufine, Dektol and Rodinal. Microdol-X had undesirable effects on the integrity of the individual grains of both emulsions. The criteria are discussed for selecting the most advantageous emulsion-developer system for the particular mode of evaluation (visual, photometric, television camera) to be applied to the finished autoradiographic specimens.", "contents": "Variation in the autoradiographic technique: I. Emulsion-developer combinations assessed by photometric measurement of single silver grains. Five commonly used developers (Acufine, Rodinal, Dektol, D19 and Microdol X) and two popular nuclear gel emulsions (Kodak NTB-2 and Ilford L4) were tested in combinations for adequacy of development characteristics and the distribution of silver particle (grain) size measured by means of a reflectance microphotometer. All developer-emulsion combinations had some range of development time during which maximum development was accomplished with no occurrence of background grains, with the exceptions of the combinations of NTB2-Acufine and NTB2-D19. Ilford L4 emulsion obtained the narrowest grain size distribution with Rodinal and Acufine, followed by Dektol, and D19. The narrowest size distribution for Kodak NTB2 emulsion was achieved with D19 followed by Acufine, Dektol and Rodinal. Microdol-X had undesirable effects on the integrity of the individual grains of both emulsions. The criteria are discussed for selecting the most advantageous emulsion-developer system for the particular mode of evaluation (visual, photometric, television camera) to be applied to the finished autoradiographic specimens.", "PMID": 977937}
{"id": "pubmed23n0032_11408", "title": "[Toxoplasmosis and interruption of pregnancy. Results of 21 parasitologic examinations].", "content": "Mouse inoculations were carried out after 21 pregnancies had been terminated for acquired toxoplasmosis. Only one positive result was obtained.", "contents": "[Toxoplasmosis and interruption of pregnancy. Results of 21 parasitologic examinations]. Mouse inoculations were carried out after 21 pregnancies had been terminated for acquired toxoplasmosis. Only one positive result was obtained.", "PMID": 977932}
{"id": "pubmed23n0032_11409", "title": "A comparative ultrastructural localization of concanavalin A, wheat germ and Ricinus communis on glomeruli of normal rat kidney.", "content": "Three lectins, concanavalin A, peroxidase labeled wheat germ and peroxidase labeled Ricinus communis have been utilized to determine the localization of various saccharide determinants in the glomerulus of the normal rat kidney. In order to obtain a homogeneous penetration of the lectins throughout the whole section, various technical parameters were studied. Only with concanavalin A, a diffuse labeling of the endoplasmic reticulum was obtained. With the three lectins, the basement membrane, mesangial matrix and the cell coat of the three cell types in the glomerulus were labeled. Differences in the labeling of the capillary wall were also noted.", "contents": "A comparative ultrastructural localization of concanavalin A, wheat germ and Ricinus communis on glomeruli of normal rat kidney. Three lectins, concanavalin A, peroxidase labeled wheat germ and peroxidase labeled Ricinus communis have been utilized to determine the localization of various saccharide determinants in the glomerulus of the normal rat kidney. In order to obtain a homogeneous penetration of the lectins throughout the whole section, various technical parameters were studied. Only with concanavalin A, a diffuse labeling of the endoplasmic reticulum was obtained. With the three lectins, the basement membrane, mesangial matrix and the cell coat of the three cell types in the glomerulus were labeled. Differences in the labeling of the capillary wall were also noted.", "PMID": 977938}
{"id": "pubmed23n0032_11410", "title": "[Perineal echotomography. A new method of objective measurement of the cervix].", "content": "Perineal echotomography is a method for measuring the dilatation of the cervix and is a good measure of the cervix during pregnancy. It permits visualization of the bag of waters, the presentation of the fetus and of the low-lying placenta.", "contents": "[Perineal echotomography. A new method of objective measurement of the cervix]. Perineal echotomography is a method for measuring the dilatation of the cervix and is a good measure of the cervix during pregnancy. It permits visualization of the bag of waters, the presentation of the fetus and of the low-lying placenta.", "PMID": 977933}
{"id": "pubmed23n0032_11411", "title": "Factors affecting the incomes of men and women physicians: an exploratory analysis.", "content": "Income differences between men and women physicians are analyzed using data from the Americal Medical Association's 1973 Eighth Periodic Survey of Physicians. While women tend to possess less favorable professional characteristics in terms of income-earning potential, the returns to many characteristics associated with higher incomes are greater for women than for men. Additional evidence on differences in weekly hours worked is presented in an effort to explain the lower incomes of women doctors.", "contents": "Factors affecting the incomes of men and women physicians: an exploratory analysis. Income differences between men and women physicians are analyzed using data from the Americal Medical Association's 1973 Eighth Periodic Survey of Physicians. While women tend to possess less favorable professional characteristics in terms of income-earning potential, the returns to many characteristics associated with higher incomes are greater for women than for men. Additional evidence on differences in weekly hours worked is presented in an effort to explain the lower incomes of women doctors.", "PMID": 977939}
{"id": "pubmed23n0032_11412", "title": "The demand for dental care: a study of consumption and household production.", "content": "Within the context of a Becker-type consumer's choice model, this paper reports an empirical study of the demand for dental care for approximately 1,000 households. The unique feature of the study is that waiting time and travel time as well as price and other traditional economic variables are entered into the regression analysis. Generally, the results confirm our expectations derived from theory, but elasticities with respect to all of the above-mentioned determinants of demand were small. In addition, some of the subtle predictions of the theory could not be confirmed.", "contents": "The demand for dental care: a study of consumption and household production. Within the context of a Becker-type consumer's choice model, this paper reports an empirical study of the demand for dental care for approximately 1,000 households. The unique feature of the study is that waiting time and travel time as well as price and other traditional economic variables are entered into the regression analysis. Generally, the results confirm our expectations derived from theory, but elasticities with respect to all of the above-mentioned determinants of demand were small. In addition, some of the subtle predictions of the theory could not be confirmed.", "PMID": 977940}
{"id": "pubmed23n0032_11413", "title": "[Attempt at evaluation of the risk of mortality in utero].", "content": "An analysis was made of the obstetric history of 1,360 patients who were delivered in 1971. It involved 3,185 pregnancies. The aim of the study was to evaluate the risk of spontaneous termination of the pregnancy, worked out from its demographic aspect. Several ideas are worthy of consideration. The risk of spontaneous intra-uterine death is cumulative, sharply increased by a repetition of such cessation of pregnancy. The same patients often undergo intentional abortion repeatedly. The respective risks of spontaneous and provoked interruption of pregnancy have been studied in greater detail as a function of parity.", "contents": "[Attempt at evaluation of the risk of mortality in utero]. An analysis was made of the obstetric history of 1,360 patients who were delivered in 1971. It involved 3,185 pregnancies. The aim of the study was to evaluate the risk of spontaneous termination of the pregnancy, worked out from its demographic aspect. Several ideas are worthy of consideration. The risk of spontaneous intra-uterine death is cumulative, sharply increased by a repetition of such cessation of pregnancy. The same patients often undergo intentional abortion repeatedly. The respective risks of spontaneous and provoked interruption of pregnancy have been studied in greater detail as a function of parity.", "PMID": 977934}
{"id": "pubmed23n0032_11414", "title": "The suppressive effect of the interferon on the ability of mouse spleen cells synthesizing IgE to sensitize rat skin for heterologous adoptive cutaneous anaphylaxis.", "content": "Mouse spleen cells which are synthesizing IgE can sensitize rat skin for HACA. When such cells are treated with mouse interferon, their ability to sensitize rat skin is reduced or completely inhibited. Two hundred PRD50 units of interferon reduced HACA reactions by more than 50%; 600 units gave complete inhibition. Interferon may act by inhibiting IgE synthesis and/or release.", "contents": "The suppressive effect of the interferon on the ability of mouse spleen cells synthesizing IgE to sensitize rat skin for heterologous adoptive cutaneous anaphylaxis. Mouse spleen cells which are synthesizing IgE can sensitize rat skin for HACA. When such cells are treated with mouse interferon, their ability to sensitize rat skin is reduced or completely inhibited. Two hundred PRD50 units of interferon reduced HACA reactions by more than 50%; 600 units gave complete inhibition. Interferon may act by inhibiting IgE synthesis and/or release.", "PMID": 977941}
{"id": "pubmed23n0032_11415", "title": "[Ligation of the internal iliac arteries in cases of placenta accreta].", "content": "Three cases of excessive hemorrhage in the third stage due to Placenta accreta are described. The authors preferred to treat these cases by ligation with catgut of the internal iliac arteries instead of hysterectomy, because the patients were young and likely to want more children. The results were very satisfactory: The hemorrhage was controlled in each case. By mean of arteriography, hysterography, and endometrial biopsy they were able to show that the I.I. arteries became patent after 3-4 months, and all the uterine functions were completely restored. Two pregnancies with normal delivery, occurred out of these 3 cases.", "contents": "[Ligation of the internal iliac arteries in cases of placenta accreta]. Three cases of excessive hemorrhage in the third stage due to Placenta accreta are described. The authors preferred to treat these cases by ligation with catgut of the internal iliac arteries instead of hysterectomy, because the patients were young and likely to want more children. The results were very satisfactory: The hemorrhage was controlled in each case. By mean of arteriography, hysterography, and endometrial biopsy they were able to show that the I.I. arteries became patent after 3-4 months, and all the uterine functions were completely restored. Two pregnancies with normal delivery, occurred out of these 3 cases.", "PMID": 977935}
{"id": "pubmed23n0032_11416", "title": "Effects of C-reactive protein on platelet function. II. Inhibition by CRP of platelet reactivities stimulated by poly-L-lysine, ADP, epinephrine, and collagen.", "content": "It was recently demonstrated that C-reactive protein (CRP)4 inhibits the response of human platelets to heataggregated human gamma-globulin and thrombin and that this inhibition is characterized by a dose-dependent reduction in aggregation, activation of platelet factor 3 (PF3), and release of beta-glucuronidase. In the present experiments, CRP was found also to inhibit the ability of washed human platelets to aggregate in response to poly-L-lysine (PLL); in these experiments, the magnitude of the inhibitory effect was dependent upon the m.w. of PLL used as the stimulating agent, and was more effective with low (15,000 daltons) than with high (400,000 daltons) m.w. polymers. CRP similarly inhibited ADP- and epinephrine-stimulated platelet aggregation in platelet-rich plasma (PRP), and this was characterized by relatively minimal suppression of the primary wave  of aggregation. CRP also inhibited the platelet aggregation induced by collagen in PRP, although it had no effect upon the adherence of platelets to collagen. Finally, CRP inhibited the activation of PF3  and the release of serotonin during stimulation of platelets with ADP, and this inhibition was temporally related to the onset of the secondary wave of aggregation. These experiments extend the platelet reactivities inhibited by CRP,  show that CRP expresses its inhibitory capacity in platelet-rich plasma as well as upon isolated platelets, raise the possibility that CRP exercises its effects by inhibiting or interfering with the release and/or utilization of endogenous platelet ADP, and support the concept that CRP plays an important role in the control of platelet responsiveness to a variety of stimuli during acute inflammatory reactions.", "contents": "Effects of C-reactive protein on platelet function. II. Inhibition by CRP of platelet reactivities stimulated by poly-L-lysine, ADP, epinephrine, and collagen. It was recently demonstrated that C-reactive protein (CRP)4 inhibits the response of human platelets to heataggregated human gamma-globulin and thrombin and that this inhibition is characterized by a dose-dependent reduction in aggregation, activation of platelet factor 3 (PF3), and release of beta-glucuronidase. In the present experiments, CRP was found also to inhibit the ability of washed human platelets to aggregate in response to poly-L-lysine (PLL); in these experiments, the magnitude of the inhibitory effect was dependent upon the m.w. of PLL used as the stimulating agent, and was more effective with low (15,000 daltons) than with high (400,000 daltons) m.w. polymers. CRP similarly inhibited ADP- and epinephrine-stimulated platelet aggregation in platelet-rich plasma (PRP), and this was characterized by relatively minimal suppression of the primary wave  of aggregation. CRP also inhibited the platelet aggregation induced by collagen in PRP, although it had no effect upon the adherence of platelets to collagen. Finally, CRP inhibited the activation of PF3  and the release of serotonin during stimulation of platelets with ADP, and this inhibition was temporally related to the onset of the secondary wave of aggregation. These experiments extend the platelet reactivities inhibited by CRP,  show that CRP expresses its inhibitory capacity in platelet-rich plasma as well as upon isolated platelets, raise the possibility that CRP exercises its effects by inhibiting or interfering with the release and/or utilization of endogenous platelet ADP, and support the concept that CRP plays an important role in the control of platelet responsiveness to a variety of stimuli during acute inflammatory reactions.", "PMID": 977942}
{"id": "pubmed23n0032_11417", "title": "Metabolic studies of guinea pig basophilic leukocytes in short-term tissue culture. I. Measurement of histaminesynthesizing capacity by using an isotopic-thin layer chromatographic assay.", "content": "Mature circulating guinea pig basophils, purified to comprise 25% or more of leukocytes, have been successfully maintained in short-term tissue culture for up to 72 hr. These cells were found to retain the ability to synthesize histamine, as assayed by a new isotopic-thin layer chromatographic assay which can reliably detect as little as 0.5 pg of 3H-histamine. Cell-associated, newly synthesized histamine was detectable as early as 1 hr of culture, was substantially increased at 6 hr, and reached maximal levels at 24 hr, when it accounted for approximately 6.5% of total cell histamine. Newly synthesized histamine was still detectable at 48 and 72 hr of culture. Histamine synthesis was decreased by lwoering the concentration of histidine in the culture medium, and was markedly reduced by all the specific histidine dedarboxylase (HDC)3 inhibitors tested, but not by alpha-methyl-DOPA, pyrilamine maleate, or metiamide. Increasing the concentration of pyridoxal phosphate, the HDC coenzyme, above that normally present in culture medium resulted in only an equivocal increase in the amount of newly synthesized histamine, whereas aminoguanidine, an inhibitor of histaminase, had no detectable effect. Uptake of exogenous histamine by cultured basophils was trivial compared to histamine synthesis. Both newly synthesized and previously manufactured, nonisotopic, histamine seemed to be stored in the same pool, as the same proportion of both was released by concanavalin A (Con A). Cellular histamine was largely conserved, with little or no spontaneous release into the medium of detectable isotopic or nonisotopic histamine. These techniques provide a model for studying granulocyte metabolic processes in vitro, and should assist in the direct investigation of a variety of their physiologic functions.", "contents": "Metabolic studies of guinea pig basophilic leukocytes in short-term tissue culture. I. Measurement of histaminesynthesizing capacity by using an isotopic-thin layer chromatographic assay. Mature circulating guinea pig basophils, purified to comprise 25% or more of leukocytes, have been successfully maintained in short-term tissue culture for up to 72 hr. These cells were found to retain the ability to synthesize histamine, as assayed by a new isotopic-thin layer chromatographic assay which can reliably detect as little as 0.5 pg of 3H-histamine. Cell-associated, newly synthesized histamine was detectable as early as 1 hr of culture, was substantially increased at 6 hr, and reached maximal levels at 24 hr, when it accounted for approximately 6.5% of total cell histamine. Newly synthesized histamine was still detectable at 48 and 72 hr of culture. Histamine synthesis was decreased by lwoering the concentration of histidine in the culture medium, and was markedly reduced by all the specific histidine dedarboxylase (HDC)3 inhibitors tested, but not by alpha-methyl-DOPA, pyrilamine maleate, or metiamide. Increasing the concentration of pyridoxal phosphate, the HDC coenzyme, above that normally present in culture medium resulted in only an equivocal increase in the amount of newly synthesized histamine, whereas aminoguanidine, an inhibitor of histaminase, had no detectable effect. Uptake of exogenous histamine by cultured basophils was trivial compared to histamine synthesis. Both newly synthesized and previously manufactured, nonisotopic, histamine seemed to be stored in the same pool, as the same proportion of both was released by concanavalin A (Con A). Cellular histamine was largely conserved, with little or no spontaneous release into the medium of detectable isotopic or nonisotopic histamine. These techniques provide a model for studying granulocyte metabolic processes in vitro, and should assist in the direct investigation of a variety of their physiologic functions.", "PMID": 977943}
{"id": "pubmed23n0032_11418", "title": "Immunologic cross-reactions between the specifically localizing cells (SLC) generated in response to sheep RBC and the red cells of other species.", "content": "Some of the lymphoid cells selectively incorporating radioactive thymidine 3 days after primary immunization with SRBC which are capable of specifically localizing in the lymph nodes of adoptively immunized syngeneic recipients challenged with SRBC (SLC-SRBC)2 are also capable of specifically localizing in lymph nodes challenged with the red blood cells of a varieth of other mammalian species. The one nonmammalian RBC tested, CRBC, failed to cross-react with SRBC by this parameter even qualitatively, although it remains possible that a feeble cross-reaction might be demonstrated with large enough experimental groups. As expected, the magnitude of the observed cross-reactions seems to vary inversely with the phylogenetic distance between the species, with ORBC showing the strongest cross-reaction (41 to 49%), ARBC showing the weakest (8 to 13%), and BRBC and HRBC occupying intermediate positions (13 to 20%). Cross-reaction between secondary anti-SRBC antibodies and the red cells of the other species were weak and inconstant, suggesting that the observed cross-reactions of specific localization may involve T cells more than B cells. During the course of these studies, it was also possible to verify experimentally our impression that with existing methods of selectively labeling SLC it is possible to study the phenomenon of specific localization using only one population of labeled cells, providing certain essential controls are employed.", "contents": "Immunologic cross-reactions between the specifically localizing cells (SLC) generated in response to sheep RBC and the red cells of other species. Some of the lymphoid cells selectively incorporating radioactive thymidine 3 days after primary immunization with SRBC which are capable of specifically localizing in the lymph nodes of adoptively immunized syngeneic recipients challenged with SRBC (SLC-SRBC)2 are also capable of specifically localizing in lymph nodes challenged with the red blood cells of a varieth of other mammalian species. The one nonmammalian RBC tested, CRBC, failed to cross-react with SRBC by this parameter even qualitatively, although it remains possible that a feeble cross-reaction might be demonstrated with large enough experimental groups. As expected, the magnitude of the observed cross-reactions seems to vary inversely with the phylogenetic distance between the species, with ORBC showing the strongest cross-reaction (41 to 49%), ARBC showing the weakest (8 to 13%), and BRBC and HRBC occupying intermediate positions (13 to 20%). Cross-reaction between secondary anti-SRBC antibodies and the red cells of the other species were weak and inconstant, suggesting that the observed cross-reactions of specific localization may involve T cells more than B cells. During the course of these studies, it was also possible to verify experimentally our impression that with existing methods of selectively labeling SLC it is possible to study the phenomenon of specific localization using only one population of labeled cells, providing certain essential controls are employed.", "PMID": 977944}
{"id": "pubmed23n0032_11419", "title": "Leukocyte-derived complement inhibitor. IV. The functional properties of C1 bound to erythrocytes pretreated with leukocyte culture supernatant.", "content": "E, pretreated with leukocyte cultures supernatant (ES), binds C1 through C1q; ES and EIgM that bind the same amount of C1 as measured in a hemolytic assay have the same uptake of 125I-C1q; ESC1q and EIgMC1q, carrying the same number of molecules of CUq per cell, have the same uptake of CUr and CUs; soluble immune compleses prevent the binding of C1 and C1q to ES. The activity of C1 bound to ES is impaired; ESC1 can react with C4 but not with C2. The C4 turnover and the C1 ING turnover by ESC1 are reduced so that ES-bound C1 is protected from destruction by C1 ING. These modifications are fully reversed when C1 is transferred from ES to EA:C1 recovers its ability to react with C2, and C1 INH. Thus the C1s activity can be modulated inside the C1 molecular complex upon binding of C1q to a lymphocyte product. In addition, the 125I-C1q uptake is proportional to the amount of IgM hemolysin used to sensitize E; it has, however, an exponential relationship to the amount of IgG or S used to sensitize E. The ratio of 125I-C1q uptake towhole C1 uptake measured in a hemolytic assay is lowerthan 2. This indicates that one molecule of IgM is sufficient to bind one molecule of C1q on E, that several molecules of IgG or S are required to bind one molecule of C1q, and that one molecule of C1q is sufficient to create a lytic site on E.", "contents": "Leukocyte-derived complement inhibitor. IV. The functional properties of C1 bound to erythrocytes pretreated with leukocyte culture supernatant. E, pretreated with leukocyte cultures supernatant (ES), binds C1 through C1q; ES and EIgM that bind the same amount of C1 as measured in a hemolytic assay have the same uptake of 125I-C1q; ESC1q and EIgMC1q, carrying the same number of molecules of CUq per cell, have the same uptake of CUr and CUs; soluble immune compleses prevent the binding of C1 and C1q to ES. The activity of C1 bound to ES is impaired; ESC1 can react with C4 but not with C2. The C4 turnover and the C1 ING turnover by ESC1 are reduced so that ES-bound C1 is protected from destruction by C1 ING. These modifications are fully reversed when C1 is transferred from ES to EA:C1 recovers its ability to react with C2, and C1 INH. Thus the C1s activity can be modulated inside the C1 molecular complex upon binding of C1q to a lymphocyte product. In addition, the 125I-C1q uptake is proportional to the amount of IgM hemolysin used to sensitize E; it has, however, an exponential relationship to the amount of IgG or S used to sensitize E. The ratio of 125I-C1q uptake towhole C1 uptake measured in a hemolytic assay is lowerthan 2. This indicates that one molecule of IgM is sufficient to bind one molecule of C1q on E, that several molecules of IgG or S are required to bind one molecule of C1q, and that one molecule of C1q is sufficient to create a lytic site on E.", "PMID": 977945}
{"id": "pubmed23n0032_11420", "title": "The metabolism of IgE. Studies in normal individuals and in a patient with IgE myeloma.", "content": "IgE metabolic turnover studies with purified radioiodinated IgE were performed in normal individuals and in a patient with IgE myeloma. The validity of the turnover studies was established in several ways, including comparisons of radioiodinated IgE turnover with the turnover of endogenously labeled 14C-IgE and with the turnover of infused unlabeled IgE in a patient with hypogammaglobulinemia. The geometric mean serum IgE concentration in 73 normal adults was 96 ng/ml with a 68% confidence interval of 24 to 386 ng/ml. Metabolic turnover studies in 10 control individuals disclosed a geometric mean total circulating IgE of 4.1 mug/kg, a mean percentage of the total exchangeable IgE in the intravascular space of 41%, a mean half-time of survival of IgE of 2.7 days, a mean fractional catabolic rate of 94% of intravascular pool per day, and a geometric mean synthetic rate of 3.8 mug/kg/day. IgE has the lowest synthetic rate and highest fractional catabolic rate of the five major classes of immunoglobulin molecules. In contrast to these normal values, a patient with IgE myeloma had a serum IgE comcentration of 42 mg/ml, a total circulating IgE of 1.7 g/kg, and a synthetic rate of 270 mg/kg/day. Furthermore, although the synthetic rate was vastly increased, the survival time was prolonged and the fractional catabolic rate was decreased to 5.1 days and 16 to 22% of the intravascular pool per day, respectively. These data are compatible with the concept that IgE is catabolized in part by a mechanism common to all immunoglobulin classes and in part by a unique mechanism not available to other immunoglobulins. At very high IgE serum concentrations, such as those encountered in patients with IgE myeloma, the unique mechanism would be saturated and only the catabolic pathways available to all immunoglobulins would be available to IgE.", "contents": "The metabolism of IgE. Studies in normal individuals and in a patient with IgE myeloma. IgE metabolic turnover studies with purified radioiodinated IgE were performed in normal individuals and in a patient with IgE myeloma. The validity of the turnover studies was established in several ways, including comparisons of radioiodinated IgE turnover with the turnover of endogenously labeled 14C-IgE and with the turnover of infused unlabeled IgE in a patient with hypogammaglobulinemia. The geometric mean serum IgE concentration in 73 normal adults was 96 ng/ml with a 68% confidence interval of 24 to 386 ng/ml. Metabolic turnover studies in 10 control individuals disclosed a geometric mean total circulating IgE of 4.1 mug/kg, a mean percentage of the total exchangeable IgE in the intravascular space of 41%, a mean half-time of survival of IgE of 2.7 days, a mean fractional catabolic rate of 94% of intravascular pool per day, and a geometric mean synthetic rate of 3.8 mug/kg/day. IgE has the lowest synthetic rate and highest fractional catabolic rate of the five major classes of immunoglobulin molecules. In contrast to these normal values, a patient with IgE myeloma had a serum IgE comcentration of 42 mg/ml, a total circulating IgE of 1.7 g/kg, and a synthetic rate of 270 mg/kg/day. Furthermore, although the synthetic rate was vastly increased, the survival time was prolonged and the fractional catabolic rate was decreased to 5.1 days and 16 to 22% of the intravascular pool per day, respectively. These data are compatible with the concept that IgE is catabolized in part by a mechanism common to all immunoglobulin classes and in part by a unique mechanism not available to other immunoglobulins. At very high IgE serum concentrations, such as those encountered in patients with IgE myeloma, the unique mechanism would be saturated and only the catabolic pathways available to all immunoglobulins would be available to IgE.", "PMID": 977946}
{"id": "pubmed23n0032_11421", "title": "Inhibition of normal allogenic lymphocyte mitogenesis by a soluble inhibitor extracted from human colonic carcinoma.", "content": "Soluble extracts from human colonic tumors (STE) and from their hepatic metastases (SHME) were found to be unable to induce a proliferative response among normal allogenic lymphocytes. However, addition of these tissue extracts to cultures stimulated with various mitogens resulted in an almost complete inhibition of lymphocyte DNA synthesis. Nevertheless, they did not reduce the unstimulated lymphocyte spontaneous proliferation. Control experiments have shown that normal or nonmalignant tissues do not affect the lymphocyte  reactivity to mitogens. The specific immunosuppressive evvect was found to be irreversible and to block lymphocyte activation at an early stage. The inhibitor was soluble (not sedimented at 220,000 times G for 2 hr) and not nonspecifically cytotoxic. STE was slso found to induce morphologic alterations resulting in blastlike cell production. However, no mitotic figures were seen, even after colchicin treatment. It is suggested that STE might contain molecular component(s) which would exert a double effect: 1) trigger metabolic alterations responsible for the blast-like cell induction, and 2) inhibit the lymphoproliferative response. The significance of such a mechanism is discussed in conection with the nonspecific immunosuppression caused by a tumor and the immune unresponsiveness against the tumor itself. A preliminiary characterization of this tumor material has shown that its molecular weight was about 70,000 and that it is not related to carcinoembryonic antigen or alpha-fetoprotein.", "contents": "Inhibition of normal allogenic lymphocyte mitogenesis by a soluble inhibitor extracted from human colonic carcinoma. Soluble extracts from human colonic tumors (STE) and from their hepatic metastases (SHME) were found to be unable to induce a proliferative response among normal allogenic lymphocytes. However, addition of these tissue extracts to cultures stimulated with various mitogens resulted in an almost complete inhibition of lymphocyte DNA synthesis. Nevertheless, they did not reduce the unstimulated lymphocyte spontaneous proliferation. Control experiments have shown that normal or nonmalignant tissues do not affect the lymphocyte  reactivity to mitogens. The specific immunosuppressive evvect was found to be irreversible and to block lymphocyte activation at an early stage. The inhibitor was soluble (not sedimented at 220,000 times G for 2 hr) and not nonspecifically cytotoxic. STE was slso found to induce morphologic alterations resulting in blastlike cell production. However, no mitotic figures were seen, even after colchicin treatment. It is suggested that STE might contain molecular component(s) which would exert a double effect: 1) trigger metabolic alterations responsible for the blast-like cell induction, and 2) inhibit the lymphoproliferative response. The significance of such a mechanism is discussed in conection with the nonspecific immunosuppression caused by a tumor and the immune unresponsiveness against the tumor itself. A preliminiary characterization of this tumor material has shown that its molecular weight was about 70,000 and that it is not related to carcinoembryonic antigen or alpha-fetoprotein.", "PMID": 977947}
{"id": "pubmed23n0032_11422", "title": "Early and late contact sensitivity reactions in guinea pigs senstitzed to oxazolone.", "content": "Inbred strain 2 and random-bred guinea pigs injected oxazolone in incomplete or complete Freund's adjuvant showed contact reactions within an hour after topical application when tested 3 weeks post-sensititization. The application when tested 3 weeks post-sensitization. The time for appearance of skin reactions at 5 days post-sensitization was 8 to 24 hr, more along classic delayed-type hypersensitivity lines. Immune serum harvested several weeks after sensititazation was effective for passive transfer of early skin reactivity, while serum at 5 to 10 days was not. Lymph node cells taken 5 days after sensitization of donors could transfer only the late skin reactivity to histocompatible recipients; lymph nodes taken at 15 days did not contain cells capable of such transfers. The heterogeneity of mechanisms involved in the production of contact dermatitis suggested by these results provides new approaches to studies in this area of allergy research.", "contents": "Early and late contact sensitivity reactions in guinea pigs senstitzed to oxazolone. Inbred strain 2 and random-bred guinea pigs injected oxazolone in incomplete or complete Freund's adjuvant showed contact reactions within an hour after topical application when tested 3 weeks post-sensititization. The application when tested 3 weeks post-sensitization. The time for appearance of skin reactions at 5 days post-sensitization was 8 to 24 hr, more along classic delayed-type hypersensitivity lines. Immune serum harvested several weeks after sensititazation was effective for passive transfer of early skin reactivity, while serum at 5 to 10 days was not. Lymph node cells taken 5 days after sensitization of donors could transfer only the late skin reactivity to histocompatible recipients; lymph nodes taken at 15 days did not contain cells capable of such transfers. The heterogeneity of mechanisms involved in the production of contact dermatitis suggested by these results provides new approaches to studies in this area of allergy research.", "PMID": 977948}
{"id": "pubmed23n0032_11423", "title": "Xenogenization of rat erythroid cells by lymphatic leukemia virus: its role in induction of autoimmune hemolytic anemia.", "content": "Newborn rats given injections of low doses of Friend lymphatic leukemia virus subsequently developed hemolytic anemia characterized by production of antierythrocyte autoantibody. Electron microscopy showed C-type virus particles budding from the cell membrane not only of lymphoid cells but also of erythrocyte percursor cells in bone marrow and spleen, suggesting that the erythroid cells were infected by the virus. In addition, erythrocyte precursor cells expressed virus-induced cell surface antigens detected by cytotoxicity tests. Normal syngeneic rats preimmunized with a Friend lymphatic leukemia virusinduced tumor and subsequently inoculated with bone marrow, spleen cells, or reticulocyte-rich fraction derived from other rats injected with high doses of the virus at birth produced cytotoxic antibody to the virus-induced tumor and antierythrocyte autoantibody. In contrast, rats subsequently inoculated with virus-infected thymus or lymph node cells produced cytotoxic antibody but not antierythrocyte autoantibody. These results indicate that \"xenogenization,\" previously shown for tumor cells and normal lymphoid cells, is also observed for rat erythroid cells and, further, that xenogenization of erythroid cells by Friend lymphatic leukemia virus is one of the most important factors in induction of autoimmune hemolytic anemia.", "contents": "Xenogenization of rat erythroid cells by lymphatic leukemia virus: its role in induction of autoimmune hemolytic anemia. Newborn rats given injections of low doses of Friend lymphatic leukemia virus subsequently developed hemolytic anemia characterized by production of antierythrocyte autoantibody. Electron microscopy showed C-type virus particles budding from the cell membrane not only of lymphoid cells but also of erythrocyte percursor cells in bone marrow and spleen, suggesting that the erythroid cells were infected by the virus. In addition, erythrocyte precursor cells expressed virus-induced cell surface antigens detected by cytotoxicity tests. Normal syngeneic rats preimmunized with a Friend lymphatic leukemia virusinduced tumor and subsequently inoculated with bone marrow, spleen cells, or reticulocyte-rich fraction derived from other rats injected with high doses of the virus at birth produced cytotoxic antibody to the virus-induced tumor and antierythrocyte autoantibody. In contrast, rats subsequently inoculated with virus-infected thymus or lymph node cells produced cytotoxic antibody but not antierythrocyte autoantibody. These results indicate that \"xenogenization,\" previously shown for tumor cells and normal lymphoid cells, is also observed for rat erythroid cells and, further, that xenogenization of erythroid cells by Friend lymphatic leukemia virus is one of the most important factors in induction of autoimmune hemolytic anemia.", "PMID": 977949}
{"id": "pubmed23n0032_11424", "title": "Activation of the alternative complement pathway by water-insoluble glucans of streptococcus mutans: the relation between their chemical structures and activating potencies.", "content": "Water insoluble, sticky glucan was synthesized by reacting glucosyltransferase obtained from the sulture filtrate of cariogenic Streptococcus mutans OMZ 176 on sucrose. This glucan (OMZ 176 glucan), that consisted of the backbone chains of consecutive alpha-1, 3 glucosidic linkages (65%) and the side chain of alpha-1, 6 glucosidic bonds (35%), showed an ability to activate the alternative pathway of the complment system in human serum. Water soluble glucans synthesized from the reaction of glycosyltransferases of S. mutans strain AHT, FA-I, and Ingbritt with sucrose, that have a high content of alpha-1,6 and a low content of alpha-1, 3 glucosidic linkages, were less effective to activate the complement system. Sephadex (G-25) and Dextran (T-2,000 and T-40), that consisted exclusively of alpha-1, 6 glucosidic linkages, did not significantly activate the complement system. Controlled Smith degradation products of OMZ 176 glucan, composed of the alpha-1, 3 linked glucose backbone chain alone, lost its activity. From these results it was concluded that both backbone chain of alpha-1, 3 glucose units and branched alpha-1, 6 glucose units were essential for the OMZ 176 glucan to activate the alternative pathway of the human complement system.", "contents": "Activation of the alternative complement pathway by water-insoluble glucans of streptococcus mutans: the relation between their chemical structures and activating potencies. Water insoluble, sticky glucan was synthesized by reacting glucosyltransferase obtained from the sulture filtrate of cariogenic Streptococcus mutans OMZ 176 on sucrose. This glucan (OMZ 176 glucan), that consisted of the backbone chains of consecutive alpha-1, 3 glucosidic linkages (65%) and the side chain of alpha-1, 6 glucosidic bonds (35%), showed an ability to activate the alternative pathway of the complment system in human serum. Water soluble glucans synthesized from the reaction of glycosyltransferases of S. mutans strain AHT, FA-I, and Ingbritt with sucrose, that have a high content of alpha-1,6 and a low content of alpha-1, 3 glucosidic linkages, were less effective to activate the complement system. Sephadex (G-25) and Dextran (T-2,000 and T-40), that consisted exclusively of alpha-1, 6 glucosidic linkages, did not significantly activate the complement system. Controlled Smith degradation products of OMZ 176 glucan, composed of the alpha-1, 3 linked glucose backbone chain alone, lost its activity. From these results it was concluded that both backbone chain of alpha-1, 3 glucose units and branched alpha-1, 6 glucose units were essential for the OMZ 176 glucan to activate the alternative pathway of the human complement system.", "PMID": 977950}
{"id": "pubmed23n0032_11425", "title": "Mechanism of the growth-promoting effect of serum albumin on concanavalin A-activated lymphocytes: protective effect of the plasma proteins.", "content": "The concentration of concanavalin A (Con A) required for optimal growth of lymphocytes is 10 to 20 times greater in the presence of plasma than in the presence of serum albumin (BSA). This shift in Con A requirement is mainly caused by Cohn fractions III and IV, which bind and probably remove free Con A. Aymphocytes, once they are activated by Con A, lose all proliferative activity unless protected by certain proteins. Cohn fraction VI and beta-lactoglobulin were found to be most effective in protecting the cells. The protective proteins do not promote growth; only BSA promotes growth of Con A-activated lymphocytes. The mechanism of the growth-promoting effect of BSA on Con A-activated lymphocytes was investigated. The continous presence of BSA in the culture medium durdly reduces, whereas protein-free medium abolishes, growth. The mechanism of the growth-promoting effect of BSA is not concerned with regulating the uptake of Con Aby lymphocytes. Red blood cells, crenated by washing in protein-free medium, revert immediately to their normal globular shape by the addition ofBSA or FAFBSA, whereas the protective proteins fail to do so, indicating a direct effect of BSA on the external surface of the erythrocyte membrane and presumably also of the lymphocyte. The hypothesis is proposed that BSA promotes growth by conveting total structural integrity to the cell surface membrane of Con A-treated lymphocytes.", "contents": "Mechanism of the growth-promoting effect of serum albumin on concanavalin A-activated lymphocytes: protective effect of the plasma proteins. The concentration of concanavalin A (Con A) required for optimal growth of lymphocytes is 10 to 20 times greater in the presence of plasma than in the presence of serum albumin (BSA). This shift in Con A requirement is mainly caused by Cohn fractions III and IV, which bind and probably remove free Con A. Aymphocytes, once they are activated by Con A, lose all proliferative activity unless protected by certain proteins. Cohn fraction VI and beta-lactoglobulin were found to be most effective in protecting the cells. The protective proteins do not promote growth; only BSA promotes growth of Con A-activated lymphocytes. The mechanism of the growth-promoting effect of BSA on Con A-activated lymphocytes was investigated. The continous presence of BSA in the culture medium durdly reduces, whereas protein-free medium abolishes, growth. The mechanism of the growth-promoting effect of BSA is not concerned with regulating the uptake of Con Aby lymphocytes. Red blood cells, crenated by washing in protein-free medium, revert immediately to their normal globular shape by the addition ofBSA or FAFBSA, whereas the protective proteins fail to do so, indicating a direct effect of BSA on the external surface of the erythrocyte membrane and presumably also of the lymphocyte. The hypothesis is proposed that BSA promotes growth by conveting total structural integrity to the cell surface membrane of Con A-treated lymphocytes.", "PMID": 977951}
{"id": "pubmed23n0032_11426", "title": "Chemotactic and bactericidal capacities of human newborn monocytes.", "content": "We evaluated the chemotactic and bactericidal capacities of human cord blood monocytes, and the ability of cord lymphocytes and sera to generate chemotactic (LDCF) and opsonic factors for monocytes. Our results suggest that the intrinsic locomotive capacity, and the receptor system for LDCF, are adequately developed in the newborn monocyte. Moreover, newborn lymphocytes appear to produce adequate amounts of LDCF, capable of attracting adult monocytes. Poor chemotaxis was observed only when cord monocytes were exposed to supernatants of cord lymphocytes, which suggests that both contribute to this abnormal response. An inhibitory factor for which only cord monosensitivity of monocytes and strength of chemotactic factor between adults and newborns would explain these results. Bactericidal capactiy of cord monocytes against Escherichia coli K-12 opsonized by either cord or adult serum was comparable to that  of adult monocytes.", "contents": "Chemotactic and bactericidal capacities of human newborn monocytes. We evaluated the chemotactic and bactericidal capacities of human cord blood monocytes, and the ability of cord lymphocytes and sera to generate chemotactic (LDCF) and opsonic factors for monocytes. Our results suggest that the intrinsic locomotive capacity, and the receptor system for LDCF, are adequately developed in the newborn monocyte. Moreover, newborn lymphocytes appear to produce adequate amounts of LDCF, capable of attracting adult monocytes. Poor chemotaxis was observed only when cord monocytes were exposed to supernatants of cord lymphocytes, which suggests that both contribute to this abnormal response. An inhibitory factor for which only cord monosensitivity of monocytes and strength of chemotactic factor between adults and newborns would explain these results. Bactericidal capactiy of cord monocytes against Escherichia coli K-12 opsonized by either cord or adult serum was comparable to that  of adult monocytes.", "PMID": 977952}
{"id": "pubmed23n0032_11427", "title": "Solubilization of IgA immune precipitates by complement.", "content": "The complement (C) system can solubilize immune precipitates prepared with antibodies of the IgG class, and apparently also of the IgM class. This paper shows that C can also solubilize immune precipitates prepared with two different antigen-binding IgA myeloma proteins. Immune precipitates were prepared with a) mouse myeloma protein TEPC 15 and PC-KLH, and b) mouse myeloma protein MOPC 315 and DNP-BSA. The precipitates could be solubilized by fresh mouse serum, but not by zymosan- or heat-inactivated serum. The rate of solubilization was not affected by the removal of of Ca++ ions. These results verify that the C-mediated solubilization effect can proceed entirely via the alternative C pathway, and lend increased support to the view that the effect is a general phenomenon, not restricted to a particular antibody class or type of antigen.", "contents": "Solubilization of IgA immune precipitates by complement. The complement (C) system can solubilize immune precipitates prepared with antibodies of the IgG class, and apparently also of the IgM class. This paper shows that C can also solubilize immune precipitates prepared with two different antigen-binding IgA myeloma proteins. Immune precipitates were prepared with a) mouse myeloma protein TEPC 15 and PC-KLH, and b) mouse myeloma protein MOPC 315 and DNP-BSA. The precipitates could be solubilized by fresh mouse serum, but not by zymosan- or heat-inactivated serum. The rate of solubilization was not affected by the removal of of Ca++ ions. These results verify that the C-mediated solubilization effect can proceed entirely via the alternative C pathway, and lend increased support to the view that the effect is a general phenomenon, not restricted to a particular antibody class or type of antigen.", "PMID": 977953}
{"id": "pubmed23n0032_11428", "title": "Generation of a neutrophil chemotactic agent by spermatozoa: role of complement and regulation by seminal plasma factors.", "content": "The interactions of polymorphonuclear leukocytes, spermatozoa, seminal plasma, and serum have been examined in an in vitro leukocyte chemotaxis system. Bovine or human spermatozoa have no neutrophil chemotactic activity alone, but generate potent activity when incubated with serum. Generation of this activity requires magnesium, but not calcium ions and is blocked by pre-heating the serum. Chemotactic activity is generated normally in serum deficient in immunoglobulins or C4, but is not produced in serum deficient in C3. Thus, spermatozoa activate complement via the alternative pathway. The chemotactically active agent has been identified as the low m.w. C5 cleavage product (C5a) on the basis of heat stability, gel filtration characteristics, and specific inhibition by anti-serum to C5. The spermatozoal constituents responsible for complement activation are largely heat stable, resistant to diisopropyfluorophosphate and on gel filtration compise a heterogeneous group of large m.w. materials. Bovine seminal plasma has no neutrophil chemotactic activity alone; considerable activity, however, is generated by incubating low, but not high concentrations of seminal plasma with serum. The chemotactic agent produced in serum is C5a. Bovine seminal plasma also contains potent heat labile inhibitors of chemotaxis. Two distinct inhibitors differing in m.w. (50 to 100,000 and 15,000) and heat stability have been isolated. The inhibitors appear to act directly on the neutrophil rather than on the chemotactic factor. Human seminal plasma exhibits slight chemotactic activity alone but does not generate significant additional activity on incubation with serum. In contrast to the inhibitory activity of bovine seminal plasma, the human material contains heat-stable, nondialyzable factors which enhance the neutrophil chemotactic response. These studies document a specific mechanism for the directed migration of polymorphonuclear leukocytes toward spermatozoa. The findings may provide an explanation for  the observed in vivo leukocyte accumulation in the female genital tract in association with spermatozoa and seminal plasma. The biologic significance of this phenomenon relates to potential leukocyte-spermatozoa interaction which may influence the likelihood of fertilization.", "contents": "Generation of a neutrophil chemotactic agent by spermatozoa: role of complement and regulation by seminal plasma factors. The interactions of polymorphonuclear leukocytes, spermatozoa, seminal plasma, and serum have been examined in an in vitro leukocyte chemotaxis system. Bovine or human spermatozoa have no neutrophil chemotactic activity alone, but generate potent activity when incubated with serum. Generation of this activity requires magnesium, but not calcium ions and is blocked by pre-heating the serum. Chemotactic activity is generated normally in serum deficient in immunoglobulins or C4, but is not produced in serum deficient in C3. Thus, spermatozoa activate complement via the alternative pathway. The chemotactically active agent has been identified as the low m.w. C5 cleavage product (C5a) on the basis of heat stability, gel filtration characteristics, and specific inhibition by anti-serum to C5. The spermatozoal constituents responsible for complement activation are largely heat stable, resistant to diisopropyfluorophosphate and on gel filtration compise a heterogeneous group of large m.w. materials. Bovine seminal plasma has no neutrophil chemotactic activity alone; considerable activity, however, is generated by incubating low, but not high concentrations of seminal plasma with serum. The chemotactic agent produced in serum is C5a. Bovine seminal plasma also contains potent heat labile inhibitors of chemotaxis. Two distinct inhibitors differing in m.w. (50 to 100,000 and 15,000) and heat stability have been isolated. The inhibitors appear to act directly on the neutrophil rather than on the chemotactic factor. Human seminal plasma exhibits slight chemotactic activity alone but does not generate significant additional activity on incubation with serum. In contrast to the inhibitory activity of bovine seminal plasma, the human material contains heat-stable, nondialyzable factors which enhance the neutrophil chemotactic response. These studies document a specific mechanism for the directed migration of polymorphonuclear leukocytes toward spermatozoa. The findings may provide an explanation for  the observed in vivo leukocyte accumulation in the female genital tract in association with spermatozoa and seminal plasma. The biologic significance of this phenomenon relates to potential leukocyte-spermatozoa interaction which may influence the likelihood of fertilization.", "PMID": 977954}
{"id": "pubmed23n0032_11429", "title": "Large-scale isolation of Dane particles from plasma containing hepatitis B antigen and deomnstration of circular double-stranded DNA molecule extruding directly from their cores.", "content": "A method was developed to isolate, on a large scale, Dane particles from five liters of pooled plasma of asymptomatic carriers of hepatitis B antigen. It involved three successive ultracentrifugation procedures; the final preparation was more than 98% pure in Dane particles. Purified Dane particles had the density of 1.23 to 1.24 g/cm3. The concentration of nucleic acids in the preparation containing purified Dane particles was too low to be detected either by chemical or by spectrophotometric method. However, a circular double-stranded DNA molecule extruding directly from the core of Dane particles was clearly demonstrated by elelctron microscopic observations. The cores of Dane particles (hepatitis B core antigen; HBCAg) were prepared by treating the Dane particle preparation with mercaptoethanol and Nonidet P-40. They were sufficient both in purity and amounts to allow the determination of antibody to HBCAg by an immune adherence hamagglutination method.", "contents": "Large-scale isolation of Dane particles from plasma containing hepatitis B antigen and deomnstration of circular double-stranded DNA molecule extruding directly from their cores. A method was developed to isolate, on a large scale, Dane particles from five liters of pooled plasma of asymptomatic carriers of hepatitis B antigen. It involved three successive ultracentrifugation procedures; the final preparation was more than 98% pure in Dane particles. Purified Dane particles had the density of 1.23 to 1.24 g/cm3. The concentration of nucleic acids in the preparation containing purified Dane particles was too low to be detected either by chemical or by spectrophotometric method. However, a circular double-stranded DNA molecule extruding directly from the core of Dane particles was clearly demonstrated by elelctron microscopic observations. The cores of Dane particles (hepatitis B core antigen; HBCAg) were prepared by treating the Dane particle preparation with mercaptoethanol and Nonidet P-40. They were sufficient both in purity and amounts to allow the determination of antibody to HBCAg by an immune adherence hamagglutination method.", "PMID": 977955}
{"id": "pubmed23n0032_11430", "title": "Specific antisera produced by immunization with precipitin lines.", "content": "Individual plasma proteins were precipitated, identified and isolated on the basis of line immunoelectrophoresis. A monospecific antibody response was induced by immunization of rabbits with less than 50 ng of precipitated antigen. Preservation of monospecificity was obtained by reimmunization with precipitates developed against the specific antisera.", "contents": "Specific antisera produced by immunization with precipitin lines. Individual plasma proteins were precipitated, identified and isolated on the basis of line immunoelectrophoresis. A monospecific antibody response was induced by immunization of rabbits with less than 50 ng of precipitated antigen. Preservation of monospecificity was obtained by reimmunization with precipitates developed against the specific antisera.", "PMID": 977958}
{"id": "pubmed23n0032_11431", "title": "Enrichment of PHA transformed lymphocytes in samples containing mixed populations.", "content": "A dextran density gradient centrifugation method has been used to separate PHA transformed lymphocytes from nontransformed lymphocytes obtained from rat blood. Fifty-four percent of the transformed lymphocytes were recovered in the fraction at a density of 1.0620 g/ml. Eighty-five percent of the cells in this fraction were transformed cells while the remainder were non-transformed lymphocytes.", "contents": "Enrichment of PHA transformed lymphocytes in samples containing mixed populations. A dextran density gradient centrifugation method has been used to separate PHA transformed lymphocytes from nontransformed lymphocytes obtained from rat blood. Fifty-four percent of the transformed lymphocytes were recovered in the fraction at a density of 1.0620 g/ml. Eighty-five percent of the cells in this fraction were transformed cells while the remainder were non-transformed lymphocytes.", "PMID": 977959}
{"id": "pubmed23n0032_11432", "title": "The fibrous proteins of stratum corneum.", "content": "The proteins of cow snout stratum corneum can be extracted in part with Tris buffer, pH 9.0, containing 6 M urea. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis shows that about half the extracted material migrates as prekeratin when the extract is treated with both SDS and mercaptoethanol; only trace amounts of the proteins migrating as prekeratin are seen when SDS alone is used. The urea extract gives precipitin lines to an antibody against prekeratin. After exhaustive extraction with the Tris-urea buffer an additional amount of protein is solubilized by extraction with buffer containing mercaptoethanol. This extract shows an electrophoretic pattern similar but not identical to prekeratin. These results suggest that the stratum corneum contains fibrous proteins with different degrees of cross-linking. An analogous system has also been observed in human stratum corneum.", "contents": "The fibrous proteins of stratum corneum. The proteins of cow snout stratum corneum can be extracted in part with Tris buffer, pH 9.0, containing 6 M urea. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis shows that about half the extracted material migrates as prekeratin when the extract is treated with both SDS and mercaptoethanol; only trace amounts of the proteins migrating as prekeratin are seen when SDS alone is used. The urea extract gives precipitin lines to an antibody against prekeratin. After exhaustive extraction with the Tris-urea buffer an additional amount of protein is solubilized by extraction with buffer containing mercaptoethanol. This extract shows an electrophoretic pattern similar but not identical to prekeratin. These results suggest that the stratum corneum contains fibrous proteins with different degrees of cross-linking. An analogous system has also been observed in human stratum corneum.", "PMID": 977985}
{"id": "pubmed23n0032_11433", "title": "Patterns of glucose catabolism during differentiation of fetal rat epidermis.", "content": "Glucose metabolism has been investigated during differentiation of fetal rat skin to evaluate shifting patterns in the catabolism of this major exogenous substrate in relation to changing requirements during epidermal maturation. Glucose utilization and aerobic glycolysis declined while oxidative metabolism increased in whole skin as stratification and cornification progressed. Activity of the hexose monophosphate shunt was quantitated in isolated epidermis for the first time. The relative activity of this pathway diminished somewhat from 20% to 12% of utilized glucose as the granular layer and stratum corneum developed. The results are consistent with (a) enhanced utilization of glucose, aerobic glycolysis, and hexose monophosphate activity in rapidly proliferating eidermis, and (b) flexibility of glucose catabolism in response to cellular requirements at different stages of differentiation.", "contents": "Patterns of glucose catabolism during differentiation of fetal rat epidermis. Glucose metabolism has been investigated during differentiation of fetal rat skin to evaluate shifting patterns in the catabolism of this major exogenous substrate in relation to changing requirements during epidermal maturation. Glucose utilization and aerobic glycolysis declined while oxidative metabolism increased in whole skin as stratification and cornification progressed. Activity of the hexose monophosphate shunt was quantitated in isolated epidermis for the first time. The relative activity of this pathway diminished somewhat from 20% to 12% of utilized glucose as the granular layer and stratum corneum developed. The results are consistent with (a) enhanced utilization of glucose, aerobic glycolysis, and hexose monophosphate activity in rapidly proliferating eidermis, and (b) flexibility of glucose catabolism in response to cellular requirements at different stages of differentiation.", "PMID": 977986}
{"id": "pubmed23n0032_11434", "title": "Arginine transferase activity in homogenates from guinea-pig hair follicles.", "content": "The transfer of arginine from tRNA to the amino terminal region of acceptor proteins has been demonstrated in postribosomal supernatant from guinea-pig hair follicle homogenate. The reaction has no requirement for template nucleic acids, ATP, GTP, or Mg++, and is unaffected by high concentrations of cycloheximide. It is concluded that the arginine transfer activity of the guinea-pig hair follicle is due to the action of a soluble enzyme similar to the arginine transferases previously described for other mammalian tissues.", "contents": "Arginine transferase activity in homogenates from guinea-pig hair follicles. The transfer of arginine from tRNA to the amino terminal region of acceptor proteins has been demonstrated in postribosomal supernatant from guinea-pig hair follicle homogenate. The reaction has no requirement for template nucleic acids, ATP, GTP, or Mg++, and is unaffected by high concentrations of cycloheximide. It is concluded that the arginine transfer activity of the guinea-pig hair follicle is due to the action of a soluble enzyme similar to the arginine transferases previously described for other mammalian tissues.", "PMID": 977987}
{"id": "pubmed23n0032_11435", "title": "Spark source mass spectrographic study of metal allergenic substances on the skin.", "content": "By spark source mass spectrography it is possible to trace very low concentrations of substances such as chromium, cobalt, and nickel in ethanol extracts of the skin. Chromium could be detected in abnormally high concentrations 3 weeks after one application, while nickel and cobalt reached background levels after 2 to 3 weeks. Touching a door handle containing chromium leads to a significant rise in the chromium content of the skin. Increased chromium content of the skin was evident in one case of wristwatch eczema as well.", "contents": "Spark source mass spectrographic study of metal allergenic substances on the skin. By spark source mass spectrography it is possible to trace very low concentrations of substances such as chromium, cobalt, and nickel in ethanol extracts of the skin. Chromium could be detected in abnormally high concentrations 3 weeks after one application, while nickel and cobalt reached background levels after 2 to 3 weeks. Touching a door handle containing chromium leads to a significant rise in the chromium content of the skin. Increased chromium content of the skin was evident in one case of wristwatch eczema as well.", "PMID": 977988}
{"id": "pubmed23n0032_11436", "title": "The induction of photocontact sensitivity in guinea pigs without UVB radiation.", "content": "Allergic photocontact sensitization could be induced in guinea pigs with 3,3',4',5-tetrachlorosalicylanilide (TCSA), 3,4',5-tribromosalicylanilide (TBS), and bithionol using pretreatment with sodium lauryl sulfate (SLS) and long-wave ultraviolet (UVA) radiation. Mid-wave ultraviolet below 320 nm (UVB) was not necessary for the induction of sensitization. Combined use of SLS pretreatment with UVA radiation resulted in more effective sensitization than combined UVB and UVA radiation. Higher sensitization rates to TCSA and TBS were achieved by allowing rest periods between each of 5 2-hr exposures to UVA than by daily 1-hr exposures for 10 consecutive days. The opposite result was obtained with bithionol. Although UVB has been customarily used in the past for induction of photosensitivity, its role is only to irritate the site of induction.", "contents": "The induction of photocontact sensitivity in guinea pigs without UVB radiation. Allergic photocontact sensitization could be induced in guinea pigs with 3,3',4',5-tetrachlorosalicylanilide (TCSA), 3,4',5-tribromosalicylanilide (TBS), and bithionol using pretreatment with sodium lauryl sulfate (SLS) and long-wave ultraviolet (UVA) radiation. Mid-wave ultraviolet below 320 nm (UVB) was not necessary for the induction of sensitization. Combined use of SLS pretreatment with UVA radiation resulted in more effective sensitization than combined UVB and UVA radiation. Higher sensitization rates to TCSA and TBS were achieved by allowing rest periods between each of 5 2-hr exposures to UVA than by daily 1-hr exposures for 10 consecutive days. The opposite result was obtained with bithionol. Although UVB has been customarily used in the past for induction of photosensitivity, its role is only to irritate the site of induction.", "PMID": 977989}
{"id": "pubmed23n0032_11437", "title": "Lymphocyte-epithelial cell interactions in oral mucosal inflammatory diseases.", "content": "Lymphocyte cytotoxicity for target cells is a method for evaluating specific lymphocyte stimulation. In vitro lymphocytotoxicity has been demonstrated in chronic ulcerative colitis and granulomatous colitis, polymyositis and dermatomyositis, systemic scleroderma, recurrent aphthous stomatitis, and periodontal disease. We have investigated lymphocytotoxicity in 36 patients with various oral inflammatory diseases using an automated cell-counting system. This investigation demonstrated in vitro lymphocytotoxicity for gingival epithelial target cells by aggressor lymphocytes harvested from patients with recurrent aphthous stomatitis and periodontal disease. This effect was not seen when the lymphocytes were harvested from normal subjects or from patients with other oral mucosal inflammatory diseases. The automated cell-counting system corresponded very well with the assay technique of exclusion of supravital dye.", "contents": "Lymphocyte-epithelial cell interactions in oral mucosal inflammatory diseases. Lymphocyte cytotoxicity for target cells is a method for evaluating specific lymphocyte stimulation. In vitro lymphocytotoxicity has been demonstrated in chronic ulcerative colitis and granulomatous colitis, polymyositis and dermatomyositis, systemic scleroderma, recurrent aphthous stomatitis, and periodontal disease. We have investigated lymphocytotoxicity in 36 patients with various oral inflammatory diseases using an automated cell-counting system. This investigation demonstrated in vitro lymphocytotoxicity for gingival epithelial target cells by aggressor lymphocytes harvested from patients with recurrent aphthous stomatitis and periodontal disease. This effect was not seen when the lymphocytes were harvested from normal subjects or from patients with other oral mucosal inflammatory diseases. The automated cell-counting system corresponded very well with the assay technique of exclusion of supravital dye.", "PMID": 977990}
{"id": "pubmed23n0032_11438", "title": "Cutaneous effects of pulsed nitrogen gas laser irradiation.", "content": "The effects of pulsed nitrogen gas laser emission (337.1 nm wavelength) were studied on human skin. The laser provides high-intensity monochromatic UVA radiation and can elicit delayed erythema in an actual exposure time of about 1 msec (105,000 pulses, each lasting 10 nsec, delivered over 210 sec). The effects of nitrogen laser irradiation were compared clinically and histologically with conventional erythemogenic UVA and UVB exposures from xenon arc or mercury arc lamps and were found to be similar in many respects. The minimal erythema dose is comparable to that obtained using more conventional continuous light sources which have more than 100 times lower intensity. A phototoxicity comparison of oral and topically applied psoralens is presented, indicating that the laser may prove useful in comparing photosenitizing capacity of certain compounds.", "contents": "Cutaneous effects of pulsed nitrogen gas laser irradiation. The effects of pulsed nitrogen gas laser emission (337.1 nm wavelength) were studied on human skin. The laser provides high-intensity monochromatic UVA radiation and can elicit delayed erythema in an actual exposure time of about 1 msec (105,000 pulses, each lasting 10 nsec, delivered over 210 sec). The effects of nitrogen laser irradiation were compared clinically and histologically with conventional erythemogenic UVA and UVB exposures from xenon arc or mercury arc lamps and were found to be similar in many respects. The minimal erythema dose is comparable to that obtained using more conventional continuous light sources which have more than 100 times lower intensity. A phototoxicity comparison of oral and topically applied psoralens is presented, indicating that the laser may prove useful in comparing photosenitizing capacity of certain compounds.", "PMID": 977991}
{"id": "pubmed23n0032_11439", "title": "Role of maternal antibody in pneumonia and bronchiolitis due to respiratory syncytial virus.", "content": "Fifteen infants with pneumonia caused by respiratory syncytial virus (RSV) and 19 infants with bronchiolitis caused by RSV were studied for the influence of homologous, circulating neutralizing antibody on the severity of their illness. All infants were under nine months of age. Although maternal neutralizing antibody did not prevent infection with RSV and illness, the severity of pneumonia caused by RSV was inversely related to the level of neutralizing antibody. The severity of bronchiolitis caused by RSV was unrelated to maternal antibody levels. Chest roentgenograms showed pneumonia to be slightly more severe than bronchiolitis. Neither the severity of illness nor the presence of maternal neutralizing antibody was related to the development of complement-fixing antibody.", "contents": "Role of maternal antibody in pneumonia and bronchiolitis due to respiratory syncytial virus. Fifteen infants with pneumonia caused by respiratory syncytial virus (RSV) and 19 infants with bronchiolitis caused by RSV were studied for the influence of homologous, circulating neutralizing antibody on the severity of their illness. All infants were under nine months of age. Although maternal neutralizing antibody did not prevent infection with RSV and illness, the severity of pneumonia caused by RSV was inversely related to the level of neutralizing antibody. The severity of bronchiolitis caused by RSV was unrelated to maternal antibody levels. Chest roentgenograms showed pneumonia to be slightly more severe than bronchiolitis. Neither the severity of illness nor the presence of maternal neutralizing antibody was related to the development of complement-fixing antibody.", "PMID": 977992}
{"id": "pubmed23n0032_11440", "title": "Radioimmunoassay for antibody to hepatitis B surface antigen shows transmission of hepatitis B virus among household contacts.", "content": "The epidemiology of infections with hepatitis B virus was studied by measurement of serum antibodies to hepatitis B surface antigen (HBS Ag) with the radioimmuno-precipitation test. Prospective studies of household contacts of HBS Ag-positive patients with acute hepatitis demonstrated that four of 41 children and one of 60 adults experienced anicteric seroconversions. Two of the 60 adults also had mild icteric hepatitis and became chronically antigenemic. Neither HBS Ag nor antibody to HBS Ag was detected in follow-up blood samples from 85 seronegative contacts of HBS Ag-negative patients with hepatitis, although seven icteric secondary cases were identified. Household contacts of HBS Ag carriers frequently had serologic evidence of exposure to hepatitis B virus (44% of adults), but the remainder had neither detectable antigen nor antibody.", "contents": "Radioimmunoassay for antibody to hepatitis B surface antigen shows transmission of hepatitis B virus among household contacts. The epidemiology of infections with hepatitis B virus was studied by measurement of serum antibodies to hepatitis B surface antigen (HBS Ag) with the radioimmuno-precipitation test. Prospective studies of household contacts of HBS Ag-positive patients with acute hepatitis demonstrated that four of 41 children and one of 60 adults experienced anicteric seroconversions. Two of the 60 adults also had mild icteric hepatitis and became chronically antigenemic. Neither HBS Ag nor antibody to HBS Ag was detected in follow-up blood samples from 85 seronegative contacts of HBS Ag-negative patients with hepatitis, although seven icteric secondary cases were identified. Household contacts of HBS Ag carriers frequently had serologic evidence of exposure to hepatitis B virus (44% of adults), but the remainder had neither detectable antigen nor antibody.", "PMID": 977993}
{"id": "pubmed23n0032_11441", "title": "Morphology, ultrastructure, and mode of division of Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma orale, and Mycoplasma salivarium.", "content": "The morphology of viable Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma orale types 1 and 2, and Mycoplasma salivarium was studied in broth cultures by interference microscopy and in thin sections by electron microscopy. Only spherical cells were seen by interference microscopy. M. hominis had a capsule-like outer layer. Except for M. orale type 1, mycoplasmas in thin sections were 0.3-1 mum in diameter, with a bounding trilaminar membrane 7.5-10 nm thick. The mycoplasmas contained DNA fibrils and randomly distributed ribosomes. No polyribosomes were seen. Dividing mycoplasmas elongated slightly; the membrane invaginated, forming one bud. Sometimes M. hominis and M. salivarium produced one bud by elongation, and the bud was attached by a tube. This method of division is not considered as characteristic but rather as due to centrifugal force separating unfixed cells during preparation for electron microscopy. Cross-septa were never observed. In thin sections M. orale type 1 was elongated and without buds, an observation which suggested that preparation for electron microscopy distorted the mycoplasmas.", "contents": "Morphology, ultrastructure, and mode of division of Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma orale, and Mycoplasma salivarium. The morphology of viable Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma orale types 1 and 2, and Mycoplasma salivarium was studied in broth cultures by interference microscopy and in thin sections by electron microscopy. Only spherical cells were seen by interference microscopy. M. hominis had a capsule-like outer layer. Except for M. orale type 1, mycoplasmas in thin sections were 0.3-1 mum in diameter, with a bounding trilaminar membrane 7.5-10 nm thick. The mycoplasmas contained DNA fibrils and randomly distributed ribosomes. No polyribosomes were seen. Dividing mycoplasmas elongated slightly; the membrane invaginated, forming one bud. Sometimes M. hominis and M. salivarium produced one bud by elongation, and the bud was attached by a tube. This method of division is not considered as characteristic but rather as due to centrifugal force separating unfixed cells during preparation for electron microscopy. Cross-septa were never observed. In thin sections M. orale type 1 was elongated and without buds, an observation which suggested that preparation for electron microscopy distorted the mycoplasmas.", "PMID": 977994}
{"id": "pubmed23n0032_11442", "title": "Antigen dose response and specificity of production of the lymphokine eosinophil stimulation promoter.", "content": "The present investigations of the recently discovered lymphokine eosinophil stimulation promoter have (1) shown that the assay system is highly sensitive and reproducible via the establishment of dose-response curves and their statistical analysis, (2) demonstrated that the direct assay is specific with use of eosinophilrich peritoneal exudate cells obtained from mice with either schistosomiasis or trichinosis, (3) demonstrated that the indirect test with splenic lymphocytes is specific with use of both the above method and radioisotope-labeled antigens, and (4) provided information on the roles of eosinophils and mononuclear cells via purification, reconstitution, and the use of antiserum to eosinophils.", "contents": "Antigen dose response and specificity of production of the lymphokine eosinophil stimulation promoter. The present investigations of the recently discovered lymphokine eosinophil stimulation promoter have (1) shown that the assay system is highly sensitive and reproducible via the establishment of dose-response curves and their statistical analysis, (2) demonstrated that the direct assay is specific with use of eosinophilrich peritoneal exudate cells obtained from mice with either schistosomiasis or trichinosis, (3) demonstrated that the indirect test with splenic lymphocytes is specific with use of both the above method and radioisotope-labeled antigens, and (4) provided information on the roles of eosinophils and mononuclear cells via purification, reconstitution, and the use of antiserum to eosinophils.", "PMID": 977995}
{"id": "pubmed23n0032_11443", "title": "The nature of aleutian disease in mink. I. Two forms of hypergammaglobulinemia as related to method of disease transmission and type of lesion.", "content": "Aleutian mink disease is generally considered to precipitate spontaneously in ranch mink, with a lethal outcome. A two-year field study of a herd of susceptible mutant mink (sapphires and violets), however, has shown that all individual mink were affected from birth; the well state consisted of periodic low-level hypergammaglobulinemia accompanied by minute vascular occlusions. The spontaneous lethal change in an individual appeared to arise during one of these hypergammaglobulinemic episodes and thus represented a failure of the immune system to control an inherent virus-induced mononucleosis. The fact that the entire herd was affected by the periodic form from birth is considered strong evidence for vertical transmission at a rate of 100%. The incidence of spontaneous precipitation was found to be dependent on the level of hypergammaglobulinemia in the mother during pregnancy.", "contents": "The nature of aleutian disease in mink. I. Two forms of hypergammaglobulinemia as related to method of disease transmission and type of lesion. Aleutian mink disease is generally considered to precipitate spontaneously in ranch mink, with a lethal outcome. A two-year field study of a herd of susceptible mutant mink (sapphires and violets), however, has shown that all individual mink were affected from birth; the well state consisted of periodic low-level hypergammaglobulinemia accompanied by minute vascular occlusions. The spontaneous lethal change in an individual appeared to arise during one of these hypergammaglobulinemic episodes and thus represented a failure of the immune system to control an inherent virus-induced mononucleosis. The fact that the entire herd was affected by the periodic form from birth is considered strong evidence for vertical transmission at a rate of 100%. The incidence of spontaneous precipitation was found to be dependent on the level of hypergammaglobulinemia in the mother during pregnancy.", "PMID": 977996}
{"id": "pubmed23n0032_11444", "title": "Synergism between amikacin and cefazolin against Klebsiella: in vitro studies and effect on the bactericidal activity of serum.", "content": "Synergism between amikacin and cefazolin was studied in vitro in 20 strains of Klebsiella isolated from clinical material. Several techniques for the demonstration of synergism in vitro were employed. The checkerboard technique with use of bactericidal end points and the killing curves method showed synergism between amikacin and cefazolin for 13 and 15 strains, respectively. The two techniques were in accordance in 12 instances. The data obtained in vitro were correlated with the bactericidal activity in sera from 10 subjects who had received the usual doses of amikacin and cefazolin, alone or in combination. The sera of subjects who received amikacin plus cefazolin were bactericidal at significantly higher dilutions than sera of patients who received cefazolin alone. The increased serum bactericidal activity in subjects receiving amikacin plus cefazolin was observed only for strains against which these antibiotics were synergistic in vitro.", "contents": "Synergism between amikacin and cefazolin against Klebsiella: in vitro studies and effect on the bactericidal activity of serum. Synergism between amikacin and cefazolin was studied in vitro in 20 strains of Klebsiella isolated from clinical material. Several techniques for the demonstration of synergism in vitro were employed. The checkerboard technique with use of bactericidal end points and the killing curves method showed synergism between amikacin and cefazolin for 13 and 15 strains, respectively. The two techniques were in accordance in 12 instances. The data obtained in vitro were correlated with the bactericidal activity in sera from 10 subjects who had received the usual doses of amikacin and cefazolin, alone or in combination. The sera of subjects who received amikacin plus cefazolin were bactericidal at significantly higher dilutions than sera of patients who received cefazolin alone. The increased serum bactericidal activity in subjects receiving amikacin plus cefazolin was observed only for strains against which these antibiotics were synergistic in vitro.", "PMID": 977997}
{"id": "pubmed23n0032_11445", "title": "beta-Lactamase activity in Chromobacterium violaceum.", "content": "A strain of Chromobacterium violaceum isolated from a fatally infected patient was found to produce a beta-lactamase. When the organism was grown in drug-free medium, beta-lactamase activity was barely detectable, but when it was grown in the presence of penicillin G, a much larger amount of activity was produced. The beta lactamase was active primarily against cephalosporins; it was sensitive to inhibition by cloxacillin but resistant to p-chloromercuribenzoate. Thus this enzyme closely resembled the common type of beta-lactamase found in Pseudomonas aeruginosa. The organism was relatively susceptible to ticarcillin, carbenicillin, and cefoxitin, which resected hydrolysis by its beta-lactamase, but was quite resistant to 11 other beta-lactam antibiotics. Production of the beta-lactamase appeared to be mediated by chromosomal genes.", "contents": "beta-Lactamase activity in Chromobacterium violaceum. A strain of Chromobacterium violaceum isolated from a fatally infected patient was found to produce a beta-lactamase. When the organism was grown in drug-free medium, beta-lactamase activity was barely detectable, but when it was grown in the presence of penicillin G, a much larger amount of activity was produced. The beta lactamase was active primarily against cephalosporins; it was sensitive to inhibition by cloxacillin but resistant to p-chloromercuribenzoate. Thus this enzyme closely resembled the common type of beta-lactamase found in Pseudomonas aeruginosa. The organism was relatively susceptible to ticarcillin, carbenicillin, and cefoxitin, which resected hydrolysis by its beta-lactamase, but was quite resistant to 11 other beta-lactam antibiotics. Production of the beta-lactamase appeared to be mediated by chromosomal genes.", "PMID": 977998}
{"id": "pubmed23n0032_11446", "title": "Distribution and characteristics of hepatitis B surface antigen in body fluids of institutionalized children and adults.", "content": "Specimens of blood, feces, urine, and nasopharyngeal washings collected at regular intervals from subjects in an institutionalized population were tested for the presence of hepatitis B surface (HBS Ag) and antibody by passive hemagglutination, radioimmunoassay, and immune electron microscopy. HBS Ag, confirmed by radioimmunoassay and immune electron microscopy, was frequently detected in nasal washings, urine, and feces of chronic carriers of HBS Ag and occasionally in subjects with recent seroconversion for HBS Ag. In addition, some subjects who had recently become positive for antibody to HBS Ag in serum had HBS Ag transiently present in urine and occasionally in the feces, without demonstrable antigenemia.", "contents": "Distribution and characteristics of hepatitis B surface antigen in body fluids of institutionalized children and adults. Specimens of blood, feces, urine, and nasopharyngeal washings collected at regular intervals from subjects in an institutionalized population were tested for the presence of hepatitis B surface (HBS Ag) and antibody by passive hemagglutination, radioimmunoassay, and immune electron microscopy. HBS Ag, confirmed by radioimmunoassay and immune electron microscopy, was frequently detected in nasal washings, urine, and feces of chronic carriers of HBS Ag and occasionally in subjects with recent seroconversion for HBS Ag. In addition, some subjects who had recently become positive for antibody to HBS Ag in serum had HBS Ag transiently present in urine and occasionally in the feces, without demonstrable antigenemia.", "PMID": 978002}
{"id": "pubmed23n0032_11447", "title": "Longitudinal studies of types A and B influenza among Seattle schoolchildren and families, 1968-74.", "content": "Longitudinal studies of influenza were conducted by postepidemic collection of sera and illness information among Seattle schoolchildren from 1968 to 1974 and in family groups from 1972 to 1974. The rate of infection with A/Hong Kong (H3N2) virus was lowest in the 1968-1969 epidemic; rates were twice as high in the epidemics of 1970 and 1972, and in the A/England (H3N2) epidemic of 1973. The introduction of a new strain of influenza type A or type B caused higher rates of infection in junior high than elementary schoolchildren. For influenza A, a shift in strain was associated with higher rates in the urban area than in the suburban area. The proportion of adolescents with serological evidence of infection who reported typical illness was 40%-42% in all three A/Hong Kong epidemics and 54% during the A/England epidemic. Reinfection with the A/Hong Kong strain was rare. Recent natural infection with the A/Hong Kong virus (1972) afforded 62% protection in the 1973 epidemic of A/England influenza.", "contents": "Longitudinal studies of types A and B influenza among Seattle schoolchildren and families, 1968-74. Longitudinal studies of influenza were conducted by postepidemic collection of sera and illness information among Seattle schoolchildren from 1968 to 1974 and in family groups from 1972 to 1974. The rate of infection with A/Hong Kong (H3N2) virus was lowest in the 1968-1969 epidemic; rates were twice as high in the epidemics of 1970 and 1972, and in the A/England (H3N2) epidemic of 1973. The introduction of a new strain of influenza type A or type B caused higher rates of infection in junior high than elementary schoolchildren. For influenza A, a shift in strain was associated with higher rates in the urban area than in the suburban area. The proportion of adolescents with serological evidence of infection who reported typical illness was 40%-42% in all three A/Hong Kong epidemics and 54% during the A/England epidemic. Reinfection with the A/Hong Kong strain was rare. Recent natural infection with the A/Hong Kong virus (1972) afforded 62% protection in the 1973 epidemic of A/England influenza.", "PMID": 978003}
{"id": "pubmed23n0032_11448", "title": "Monocyte function in infectious mononucleosis: evidence for a reversible cellular defect.", "content": "Migration of blood monocytes from patients with acute infectious mononucleosis and from normal controls was measured against chemotactic factors in serum. Moncytes from patients with acute infectious mononucleosis showed decreased migration as compared with that of control monocytes. However, serum from patients with infectious mononucleosis contained normal or above normal amounts of chemotaxins for monocytes. The migratory defect of monocytes from patients with infectious mononucleosis was reversible within three months after the onset of diesease. The cause of this monocyte migration defect in infectious mononucleosis is though to be an in vivo blockade of receptors on monocytes for chemotaxins, and it is speculated that this defect can partially explain the explain the ablated delayed-hypersensitivity skin reactions in this disease.", "contents": "Monocyte function in infectious mononucleosis: evidence for a reversible cellular defect. Migration of blood monocytes from patients with acute infectious mononucleosis and from normal controls was measured against chemotactic factors in serum. Moncytes from patients with acute infectious mononucleosis showed decreased migration as compared with that of control monocytes. However, serum from patients with infectious mononucleosis contained normal or above normal amounts of chemotaxins for monocytes. The migratory defect of monocytes from patients with infectious mononucleosis was reversible within three months after the onset of diesease. The cause of this monocyte migration defect in infectious mononucleosis is though to be an in vivo blockade of receptors on monocytes for chemotaxins, and it is speculated that this defect can partially explain the explain the ablated delayed-hypersensitivity skin reactions in this disease.", "PMID": 978004}
{"id": "pubmed23n0032_11449", "title": "Effect of serum and nasal neutralizing antibodies on bovine respiratory syncytial virus infection in calves.", "content": "Calves exposed to a bovine respiratory syncytial virus showed mild clinical signs of respiratory illness and responded serologically. The disease occurred in the presence or absence of circulating antibodies, but there was no evidence of exacerbation of the disease due to preexisting serum antibody. Nasal secretory antibody appeared to protect the calves against the disease. Calves previously exposed to the virus were immune to challenge. The virus was recovered at a high frequency when specimens of nasal secretion (not subjected to freezing and thawing) were inoculated into susceptible cells within 1 hr after collection.", "contents": "Effect of serum and nasal neutralizing antibodies on bovine respiratory syncytial virus infection in calves. Calves exposed to a bovine respiratory syncytial virus showed mild clinical signs of respiratory illness and responded serologically. The disease occurred in the presence or absence of circulating antibodies, but there was no evidence of exacerbation of the disease due to preexisting serum antibody. Nasal secretory antibody appeared to protect the calves against the disease. Calves previously exposed to the virus were immune to challenge. The virus was recovered at a high frequency when specimens of nasal secretion (not subjected to freezing and thawing) were inoculated into susceptible cells within 1 hr after collection.", "PMID": 978005}
{"id": "pubmed23n0032_11450", "title": "Effect of alveolar lining material on phagocytic and bactericidal activity of lung macrophages against Staphylococcus aureau.", "content": "Unstimulated rabbits were sacrificed and their lungs washed with heparinized saline. After alveolar macrophages were harvested, the cell-free lavage fluid was centrifuged at 47,000 X g to recover a small, whitish, surface-active pellet (F fraction.) The supernatant was concentrated 15-fold by vacuum dialysis (P fraction). Alveolar macrophages in a serum-free system were challenged with radiolabeled (32P) Staphylococcus aureus preincubated in either balanced salt solution or F or P fraction. A small increase in alveolar macrophage bacterial uptake occurred with P fraction-treated staphylococci. P fraction from locally immunized animals further enhanced phagocytosis. In bactericidial experiments, alveolar macrophages were allowed to phagocytize staphylococci preincubated in either balanced salt solution or F fraction. Intracellular bactericidal activity of alveolar macrophages was quantitated by lysotaphin lysis of extracellular bacteria and quantitation of viable intracellular bacteria. Enhanced lung macrophage bactericidal activity against F fraction-incubated staphylococci was noted.", "contents": "Effect of alveolar lining material on phagocytic and bactericidal activity of lung macrophages against Staphylococcus aureau. Unstimulated rabbits were sacrificed and their lungs washed with heparinized saline. After alveolar macrophages were harvested, the cell-free lavage fluid was centrifuged at 47,000 X g to recover a small, whitish, surface-active pellet (F fraction.) The supernatant was concentrated 15-fold by vacuum dialysis (P fraction). Alveolar macrophages in a serum-free system were challenged with radiolabeled (32P) Staphylococcus aureus preincubated in either balanced salt solution or F or P fraction. A small increase in alveolar macrophage bacterial uptake occurred with P fraction-treated staphylococci. P fraction from locally immunized animals further enhanced phagocytosis. In bactericidial experiments, alveolar macrophages were allowed to phagocytize staphylococci preincubated in either balanced salt solution or F fraction. Intracellular bactericidal activity of alveolar macrophages was quantitated by lysotaphin lysis of extracellular bacteria and quantitation of viable intracellular bacteria. Enhanced lung macrophage bactericidal activity against F fraction-incubated staphylococci was noted.", "PMID": 978037}
{"id": "pubmed23n0032_11451", "title": "Mechanism of the increased splenic erythropoiesis in mice treated with estradiol benzoate.", "content": "Pharmacological doses of estrogens induce osteosclerosis of the bone marrow, and depress colony-forming units (CFU's) and platelet and leukocyte counts in mice. A compensatoryincreasts in mice. A compensatory increase in splenic erythropoiesis prevents a fall in the hematocrit. The mechanism of this compensation was investigated as follows, BDF1 female mice were injected subcutaneously thrice weekly for 6 weeks wiht 50 mugg of estradiol benzoate (EB) or sesame oil (SO). Subsequently the hematocrit, red cell mass (RCM), and 4 hour per cent of 59Fe uptake into the femurs and spleens were determined in groups of five mice for 4 consecutive days. The RCM and hematocrit were not significantly different in the two groups. The per cent of 59Fe uptake into the femurs of EB-treated mice was less than 30 per cent of that in SO-treated mice and the per cent of 59Fe uptake into the spleens of EB mice was more than two times that in SO mice. To ascertain whether the increase in plenic erythropoiesis resulted from an increase in the number of splenic erythropoietin-responsive cells (ERC), the 4 hours per cent 59Fe uptake into the spleen was determined in continuously hypertransfused EB and SO mice injected with erythropoietin (Ep). Whereas hypertransfuction depressed the splenic per cent of 59Fe uptake in EB and SO mice equally, injection of Ep increased the per cent of 59Fe uptake into the spleens of of EB mice to greater than two times that of SO mice. Next, the plasma Ep level of mice injected with EB or SO for 2, 4, or 6 weeks was determined after exposure of the animals to hypoxia. Ep titers were greater than three times higher in EB mice than in SO mice, We conclude that at least two mechanisms act to cause the compensatory increase in splenic erythropoiesis after marrow suppression by EB: (1) the Ep levels rise and (2) the splenic ERC population increases. The latter is probably not due to the increased plasma Ep level because it also occurs in mice whose Ep production is suppressed by plethora.", "contents": "Mechanism of the increased splenic erythropoiesis in mice treated with estradiol benzoate. Pharmacological doses of estrogens induce osteosclerosis of the bone marrow, and depress colony-forming units (CFU's) and platelet and leukocyte counts in mice. A compensatoryincreasts in mice. A compensatory increase in splenic erythropoiesis prevents a fall in the hematocrit. The mechanism of this compensation was investigated as follows, BDF1 female mice were injected subcutaneously thrice weekly for 6 weeks wiht 50 mugg of estradiol benzoate (EB) or sesame oil (SO). Subsequently the hematocrit, red cell mass (RCM), and 4 hour per cent of 59Fe uptake into the femurs and spleens were determined in groups of five mice for 4 consecutive days. The RCM and hematocrit were not significantly different in the two groups. The per cent of 59Fe uptake into the femurs of EB-treated mice was less than 30 per cent of that in SO-treated mice and the per cent of 59Fe uptake into the spleens of EB mice was more than two times that in SO mice. To ascertain whether the increase in plenic erythropoiesis resulted from an increase in the number of splenic erythropoietin-responsive cells (ERC), the 4 hours per cent 59Fe uptake into the spleen was determined in continuously hypertransfused EB and SO mice injected with erythropoietin (Ep). Whereas hypertransfuction depressed the splenic per cent of 59Fe uptake in EB and SO mice equally, injection of Ep increased the per cent of 59Fe uptake into the spleens of of EB mice to greater than two times that of SO mice. Next, the plasma Ep level of mice injected with EB or SO for 2, 4, or 6 weeks was determined after exposure of the animals to hypoxia. Ep titers were greater than three times higher in EB mice than in SO mice, We conclude that at least two mechanisms act to cause the compensatory increase in splenic erythropoiesis after marrow suppression by EB: (1) the Ep levels rise and (2) the splenic ERC population increases. The latter is probably not due to the increased plasma Ep level because it also occurs in mice whose Ep production is suppressed by plethora.", "PMID": 978038}
{"id": "pubmed23n0032_11452", "title": "Patterns of iron storage in dietary iron overload and idiopathic hemochromatosis.", "content": "Bone marrow iron stores rise in proportion to the total body iron store in dietary iron overload. The situation in the genetic disorder of idiopathic hemochromatosis is not as clear. A method for measuring the storage iron concentration chemically on samples of bone marrow obtained by trephine needle biopsy was therefore developed. Its value as a measure of tissue iron stores was established in a preliminary investigation in which specimens of liver, spleen, and bone marrow were obtained at necropsies on 66 South African Negroes among whom dietary iron overload is common. A wide range of nonheme iron concentrations was found, but in each individual there was a highly significant correlation between the concentrations in the three tissues. Nonheme iron concentrations were then determined on trephine bone marrow biopsy specimens from eight Caucasian patients with untreated idiopathic hemochromatosis, and on percutaneous liver biopsy specimens from four of them. The concentrations in the livers were in the anticipated range of 5,000 mug per gram wet weight (2 per cent dry weight). In contrast the geometric mean value for bone marrow iron concentration was 186 mug per gram wet weight, a figure that fell below the fiftieth percentile for marrow iron concentrations in the South African Negroes, whereas the geometric mean liver iron concentration was above the ninetieth percentile. These findings indicate that subjects with idiopathic hemochromatosis whose liver iron stores are grossly increased do not show a comparable rise in bone marrow iron stores.", "contents": "Patterns of iron storage in dietary iron overload and idiopathic hemochromatosis. Bone marrow iron stores rise in proportion to the total body iron store in dietary iron overload. The situation in the genetic disorder of idiopathic hemochromatosis is not as clear. A method for measuring the storage iron concentration chemically on samples of bone marrow obtained by trephine needle biopsy was therefore developed. Its value as a measure of tissue iron stores was established in a preliminary investigation in which specimens of liver, spleen, and bone marrow were obtained at necropsies on 66 South African Negroes among whom dietary iron overload is common. A wide range of nonheme iron concentrations was found, but in each individual there was a highly significant correlation between the concentrations in the three tissues. Nonheme iron concentrations were then determined on trephine bone marrow biopsy specimens from eight Caucasian patients with untreated idiopathic hemochromatosis, and on percutaneous liver biopsy specimens from four of them. The concentrations in the livers were in the anticipated range of 5,000 mug per gram wet weight (2 per cent dry weight). In contrast the geometric mean value for bone marrow iron concentration was 186 mug per gram wet weight, a figure that fell below the fiftieth percentile for marrow iron concentrations in the South African Negroes, whereas the geometric mean liver iron concentration was above the ninetieth percentile. These findings indicate that subjects with idiopathic hemochromatosis whose liver iron stores are grossly increased do not show a comparable rise in bone marrow iron stores.", "PMID": 978039}
{"id": "pubmed23n0032_11453", "title": "Cholesterol dynamics in autoimmune hyperlipidemia.", "content": "A 69-year-old white male with autoimmune hyperlipidemia for 19 years characterized by high serum levels (1,5000 to 3,400 mg. per deciliter) of IgA firmly bound to very-low- and low-density lipoproteins (serum total cholesterol 852 +/- 51 mg./dl., free cholesterol 340 +/- 52, triglyceride 1638 +/- 411, phospholipid 934 +/- 84) received intravenously a tracer dose of cholesterol-4-14C. Serum cholesterol specific activity was followed for 337 days and analyzed by two methods: (1) compartmental analysis which revealed the best fit of a two-compartment model with rapidly exchangeable pool 710 gm. (2,563 per cent of the mean of 15 normal subjects), slowly exchangeable pool 317 gm. (651 per cent), mean transit time 92.5 days (167 per cent), turnover rate 9.23 gm. per day (654 per cent), and excretory coefficient 0.013 (25 per cent); (2) a simulated five-compartment model involving serum free, esterified, red blood cell, and rapidly and slowly exchangeable tissue cholesterols for which pool sizes of 17, 25, 2.4, 674, and 350 gm., respectively, were calculated and a turnover rate of 9.44 gm./day agreed well with that of the two-compartment model. The extreme hyperlipoproteinemia and expanded body cholesterol pools were primarily due to the impairment of feedback control of cholesterol synthesis as a consequence of the complexing of lipoprotein and IgA.", "contents": "Cholesterol dynamics in autoimmune hyperlipidemia. A 69-year-old white male with autoimmune hyperlipidemia for 19 years characterized by high serum levels (1,5000 to 3,400 mg. per deciliter) of IgA firmly bound to very-low- and low-density lipoproteins (serum total cholesterol 852 +/- 51 mg./dl., free cholesterol 340 +/- 52, triglyceride 1638 +/- 411, phospholipid 934 +/- 84) received intravenously a tracer dose of cholesterol-4-14C. Serum cholesterol specific activity was followed for 337 days and analyzed by two methods: (1) compartmental analysis which revealed the best fit of a two-compartment model with rapidly exchangeable pool 710 gm. (2,563 per cent of the mean of 15 normal subjects), slowly exchangeable pool 317 gm. (651 per cent), mean transit time 92.5 days (167 per cent), turnover rate 9.23 gm. per day (654 per cent), and excretory coefficient 0.013 (25 per cent); (2) a simulated five-compartment model involving serum free, esterified, red blood cell, and rapidly and slowly exchangeable tissue cholesterols for which pool sizes of 17, 25, 2.4, 674, and 350 gm., respectively, were calculated and a turnover rate of 9.44 gm./day agreed well with that of the two-compartment model. The extreme hyperlipoproteinemia and expanded body cholesterol pools were primarily due to the impairment of feedback control of cholesterol synthesis as a consequence of the complexing of lipoprotein and IgA.", "PMID": 978040}
{"id": "pubmed23n0032_11454", "title": "Reversible neutrophil defect in patients with bacterial endocarditis.", "content": "The bactericidal capacities of neutrophilic polymorphonuclear neukocytes (PMN) from five untreated adults with bacterial endocarditis (BE), eight untreated patients with other forms of acute bacterial infection (ABI), and fourteen drug-free uniinfected normal subjects (NS) were compared with a new technique that uses five increasing bacteria-to-neutrophil ratios ranging from 1.25 to 100 Staphylococcus aureus per neutrophil. PMN from uninfected NS or untreated patients with ABI demonstrated a similar and reproducible ability to kill increasing numbers of S. aureus in 8 per cent normal serum. In contrast, the bactericidal activities of the PMN from untreated patients with BE were significantly depressed and the defect was more apparent at high ratios. Neutrophils from some of these individuals had decreased bactericidal action only at high ratios, indicating a quantitative type of neutrophil defect. There was no morphologic deficiency in the uptake of bacteria by BE neutrophils and comparable rates of glucose [-1-14C] oxidation were found in BE and control neutrophils stimulated with various ratios of heat-killed bacteria. Therefore, the observed abnormality appeared to be that of intracellular killing rather than of ingestion. The proportions of bacteria killed by the MN of untreated patients with BE improved after antibiotic treatment and became equal to those of NS or untreated ABI patients. This rapid return to normal bactericidal function by the PMN during treatment indicates that the prior deficiency was an acquired consequence of the infectious process of BE.", "contents": "Reversible neutrophil defect in patients with bacterial endocarditis. The bactericidal capacities of neutrophilic polymorphonuclear neukocytes (PMN) from five untreated adults with bacterial endocarditis (BE), eight untreated patients with other forms of acute bacterial infection (ABI), and fourteen drug-free uniinfected normal subjects (NS) were compared with a new technique that uses five increasing bacteria-to-neutrophil ratios ranging from 1.25 to 100 Staphylococcus aureus per neutrophil. PMN from uninfected NS or untreated patients with ABI demonstrated a similar and reproducible ability to kill increasing numbers of S. aureus in 8 per cent normal serum. In contrast, the bactericidal activities of the PMN from untreated patients with BE were significantly depressed and the defect was more apparent at high ratios. Neutrophils from some of these individuals had decreased bactericidal action only at high ratios, indicating a quantitative type of neutrophil defect. There was no morphologic deficiency in the uptake of bacteria by BE neutrophils and comparable rates of glucose [-1-14C] oxidation were found in BE and control neutrophils stimulated with various ratios of heat-killed bacteria. Therefore, the observed abnormality appeared to be that of intracellular killing rather than of ingestion. The proportions of bacteria killed by the MN of untreated patients with BE improved after antibiotic treatment and became equal to those of NS or untreated ABI patients. This rapid return to normal bactericidal function by the PMN during treatment indicates that the prior deficiency was an acquired consequence of the infectious process of BE.", "PMID": 978041}
{"id": "pubmed23n0032_11455", "title": "Severe staphylococcal disease associated with allergic manifestations, hyperimmunoglobulinemia E, and defective neutrophil chemotaxis.", "content": "Neutrophil granulocyte function was determined in three patients with systemic staphylococcal infection, clinical manifestations of generalized allergic disease, and hyperimmunoglobulinemia E. Each of the patients had urticarial skin rashes before or at the time of development of staphylococcal suppurative lymphadenitis, pneumonia, or sepsis. Neutrophil chemotaxis, random migration, phagocytosis, and bactericidal capacity were assessed to determine if an abnormality in these functions might have contributed to the development of severe staphylococcal infections. Each of the three patients with generalized urticaria was found to have a marked defect in neutrophil chemotaxis. The mean chemotactic index of the patients was 12 +/- 4, whereas that of 20 controls was 72 +/- 11. Neutrophil random migration, phagocytosis, and bactericidal capacity were normal in each patient. The serum or plasma of the patients did not inhibit chemotaxis of control neutrophils and did not contain an increased concentration of the chemotactic-factor inactivator found in normal serum. Treatment of the neutrophils of these three patients with the competitive histamine H2 receptor blocking agent, burimamide, produced a significant increase in chemotactic responsiveness. These studies suggest the possibility of pharmacologic modification of neutrophil granulocyte function.", "contents": "Severe staphylococcal disease associated with allergic manifestations, hyperimmunoglobulinemia E, and defective neutrophil chemotaxis. Neutrophil granulocyte function was determined in three patients with systemic staphylococcal infection, clinical manifestations of generalized allergic disease, and hyperimmunoglobulinemia E. Each of the patients had urticarial skin rashes before or at the time of development of staphylococcal suppurative lymphadenitis, pneumonia, or sepsis. Neutrophil chemotaxis, random migration, phagocytosis, and bactericidal capacity were assessed to determine if an abnormality in these functions might have contributed to the development of severe staphylococcal infections. Each of the three patients with generalized urticaria was found to have a marked defect in neutrophil chemotaxis. The mean chemotactic index of the patients was 12 +/- 4, whereas that of 20 controls was 72 +/- 11. Neutrophil random migration, phagocytosis, and bactericidal capacity were normal in each patient. The serum or plasma of the patients did not inhibit chemotaxis of control neutrophils and did not contain an increased concentration of the chemotactic-factor inactivator found in normal serum. Treatment of the neutrophils of these three patients with the competitive histamine H2 receptor blocking agent, burimamide, produced a significant increase in chemotactic responsiveness. These studies suggest the possibility of pharmacologic modification of neutrophil granulocyte function.", "PMID": 978042}
{"id": "pubmed23n0032_11456", "title": "Effects of varying sodium intake on blood pressure and renin-angiotensin system in subtotally nephrectomized rats.", "content": "In rats in which the renal mass had been reduced by 70 per cent, the effects of varying sodium intake on blood pressure, serum electrolytes, renin-angiotensin system, and some other parameters that were modified simultaneously were studied. Within 4 weeks, a high sodium diet (750 mEa. per kilogram) resulted in marked hypertension, whereas a standard sodium diet (150 mEq. per kilogram) elevated the blood pressure only slightly. A low sodium diet (less than 0.2 mEq. per kilogram) prevented the rise in blood pressure. In the hypertensive group, the hematocrit values were markedly decreased, indicating the expansion of extracellular and intravascular spaces. The compensatory renal hypertrophy was accelerated by the high sodium diet and retarded during restriction. During low sodium intake, the serum concentration of sodium was diminished and that of potassium elevated. During the high sodium diet, the sodium concentration was unchanged, but the potassium concentration was decreased. Subtotal nephrectomy diminished the plasma angiotensin II concentration, and the renin content of the kidney remnant was lower than that of the kidneys from control animals. Sodium restriction stimulated the renin angiotensin system markedly, whereas high sodium intake suppressed it. After subtotal nephrectomy, elevation of blood pressure, renal hypertrophy, and suppression of the renin-angiotensin system are closely related to sodium intake.", "contents": "Effects of varying sodium intake on blood pressure and renin-angiotensin system in subtotally nephrectomized rats. In rats in which the renal mass had been reduced by 70 per cent, the effects of varying sodium intake on blood pressure, serum electrolytes, renin-angiotensin system, and some other parameters that were modified simultaneously were studied. Within 4 weeks, a high sodium diet (750 mEa. per kilogram) resulted in marked hypertension, whereas a standard sodium diet (150 mEq. per kilogram) elevated the blood pressure only slightly. A low sodium diet (less than 0.2 mEq. per kilogram) prevented the rise in blood pressure. In the hypertensive group, the hematocrit values were markedly decreased, indicating the expansion of extracellular and intravascular spaces. The compensatory renal hypertrophy was accelerated by the high sodium diet and retarded during restriction. During low sodium intake, the serum concentration of sodium was diminished and that of potassium elevated. During the high sodium diet, the sodium concentration was unchanged, but the potassium concentration was decreased. Subtotal nephrectomy diminished the plasma angiotensin II concentration, and the renin content of the kidney remnant was lower than that of the kidneys from control animals. Sodium restriction stimulated the renin angiotensin system markedly, whereas high sodium intake suppressed it. After subtotal nephrectomy, elevation of blood pressure, renal hypertrophy, and suppression of the renin-angiotensin system are closely related to sodium intake.", "PMID": 978043}
{"id": "pubmed23n0032_11457", "title": "Plasmic degradation of fibrinogen Paris I.", "content": "Fibrin obtained from the plasma of a patient having abnormal fibrogen Paris I contains normal alpha, beta, and gamma polypeptide chains as well as an abnormal gamma-chain (gammaParis I) of approximately 51,000 daltons molecular weight. Plasmic digestion of Paris I fibrogen and noncrosslinked fibrin yields both normal and abnormal Fragment D molecules, the latter having a higher negative charge and molecular weight than that liberated from normal fibrinogen and noncorsslinked fibrin. After disulfide bond reduction, an abnormal polypeptide chain of approximately 40,500 +/- 2,000 daltons molecular weight was demonstrated in the Paris I digests by dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Comparison with the electrophoretic pattern for reduced digests of normal substrates indicates that it is a gamma-chain remnant in the normal Fragment D. Although the carbohydrate content in the gamma-Paris I-chain is slightly higher than that in the normal gamma-chain, as measured by periodic acid-Schiff reagent (PAS) staining intensity, it is concluded that extra carbohydrate does not account for the high molecular weight of the gamma-Paris I-chain since the 40,500 dalton chain does not stain with PAS. Plasma digestion of Paris I crosslinked fibrin yields a large amount of Fragment D in addition to Fragment D-D (\"D-dimer\") and E molecules, in contrast to a digest of normal crosslinked fibrin, from which only the latter two fragments are formed. This finding suggests that the defect in fibrinogen Paris I derives from an abnormality in the carboxy-terminal region of the gammaParis I-chain, so that in the presence of Factor XIII, these chains are not crosslinked and Fragment D-D molecules are not liberated upon subsequent plasmic degradation. The data provide support for the previous conclusion that a longer than normal polypeptide chain sequence at the carboxy-terminal portion of the gammaParis I-chains accounts for the increased size of these chains relative to the normal gamma-chains and for the abnormal function of the intact fibrinogen Paris I molecule.", "contents": "Plasmic degradation of fibrinogen Paris I. Fibrin obtained from the plasma of a patient having abnormal fibrogen Paris I contains normal alpha, beta, and gamma polypeptide chains as well as an abnormal gamma-chain (gammaParis I) of approximately 51,000 daltons molecular weight. Plasmic digestion of Paris I fibrogen and noncrosslinked fibrin yields both normal and abnormal Fragment D molecules, the latter having a higher negative charge and molecular weight than that liberated from normal fibrinogen and noncorsslinked fibrin. After disulfide bond reduction, an abnormal polypeptide chain of approximately 40,500 +/- 2,000 daltons molecular weight was demonstrated in the Paris I digests by dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Comparison with the electrophoretic pattern for reduced digests of normal substrates indicates that it is a gamma-chain remnant in the normal Fragment D. Although the carbohydrate content in the gamma-Paris I-chain is slightly higher than that in the normal gamma-chain, as measured by periodic acid-Schiff reagent (PAS) staining intensity, it is concluded that extra carbohydrate does not account for the high molecular weight of the gamma-Paris I-chain since the 40,500 dalton chain does not stain with PAS. Plasma digestion of Paris I crosslinked fibrin yields a large amount of Fragment D in addition to Fragment D-D (\"D-dimer\") and E molecules, in contrast to a digest of normal crosslinked fibrin, from which only the latter two fragments are formed. This finding suggests that the defect in fibrinogen Paris I derives from an abnormality in the carboxy-terminal region of the gammaParis I-chain, so that in the presence of Factor XIII, these chains are not crosslinked and Fragment D-D molecules are not liberated upon subsequent plasmic degradation. The data provide support for the previous conclusion that a longer than normal polypeptide chain sequence at the carboxy-terminal portion of the gammaParis I-chains accounts for the increased size of these chains relative to the normal gamma-chains and for the abnormal function of the intact fibrinogen Paris I molecule.", "PMID": 978044}
{"id": "pubmed23n0032_11458", "title": "Evaluation of a new automatic device for taking and recording blood pressures.", "content": "A new instrument capable of recording systolic and diastolic blood pressure automatically and repetitively is described, and its accuracy is shown to be equal to that obtained by a carefully trained observer among 53 patients with different levels of blood pressure. The instrument (BosoMat) is also shown to be more accurate than two others available, namely, the Arteriosonde Model 1213 and the SphygmoStat Model B-300. The standard deviation among three consecutive blood pressures on the same patient as determined by the new instrument or by the nurse using traditional methods is shown to be approximately 4 mm. Hg.", "contents": "Evaluation of a new automatic device for taking and recording blood pressures. A new instrument capable of recording systolic and diastolic blood pressure automatically and repetitively is described, and its accuracy is shown to be equal to that obtained by a carefully trained observer among 53 patients with different levels of blood pressure. The instrument (BosoMat) is also shown to be more accurate than two others available, namely, the Arteriosonde Model 1213 and the SphygmoStat Model B-300. The standard deviation among three consecutive blood pressures on the same patient as determined by the new instrument or by the nurse using traditional methods is shown to be approximately 4 mm. Hg.", "PMID": 978045}
{"id": "pubmed23n0032_11459", "title": "Effect of hydrocortisone on total body calcium in rats.", "content": "Administration of 5 mg. of hydrocortisone acetate to rats every other day for 2 weeks resulted in growth retardation and weight loss as indicated by body weights of experimental animals, which averaged 33 per cent lower than those of the controls, and a significant decrease in the length of the tibiae and femurs (p less than 0.01 for treated vs controls). However, despite the smaller size of the treated animals, the values for total body calcium (TBCa) and the calcium in the tibia and femur did not differ significantly from control values. Thus, there was more calcium per unit length of bone, resulting in an increase in the skeletal density of treated rats. This finding was confirmed by x-ray examination of these bones. The net intestinal absorption of calcium (rate of initial entry) calculated from plasma levels following an oral and intravenous dose of 47Ca and 85Sr, respectively, was not significantly different in hydrocortisone-treated rats compared to controls. This would indicate that the rate of intestinal absorption of calcium is unimpaired despite the administration of massive doses and corticosteroids. When the animals were placed on a calcium-deficient diet, both TBCa and tibia and femur calcium levels were decreased. Subsequent administration and hydrocortisone did not alter the calcium values. The results of this study are compatible with the hypothesis that hydrocortisone promotes weight loss, retards growth, but inhibits the rate of bone resorption.", "contents": "Effect of hydrocortisone on total body calcium in rats. Administration of 5 mg. of hydrocortisone acetate to rats every other day for 2 weeks resulted in growth retardation and weight loss as indicated by body weights of experimental animals, which averaged 33 per cent lower than those of the controls, and a significant decrease in the length of the tibiae and femurs (p less than 0.01 for treated vs controls). However, despite the smaller size of the treated animals, the values for total body calcium (TBCa) and the calcium in the tibia and femur did not differ significantly from control values. Thus, there was more calcium per unit length of bone, resulting in an increase in the skeletal density of treated rats. This finding was confirmed by x-ray examination of these bones. The net intestinal absorption of calcium (rate of initial entry) calculated from plasma levels following an oral and intravenous dose of 47Ca and 85Sr, respectively, was not significantly different in hydrocortisone-treated rats compared to controls. This would indicate that the rate of intestinal absorption of calcium is unimpaired despite the administration of massive doses and corticosteroids. When the animals were placed on a calcium-deficient diet, both TBCa and tibia and femur calcium levels were decreased. Subsequent administration and hydrocortisone did not alter the calcium values. The results of this study are compatible with the hypothesis that hydrocortisone promotes weight loss, retards growth, but inhibits the rate of bone resorption.", "PMID": 978046}
{"id": "pubmed23n0032_11460", "title": "The dexamethasone-modified adrenal scintiscan in hyporeninemic aldosteronism (tumor versus hyperplasia). A comparison with adrenal venography and adrenal venous aldosterone.", "content": "The dexamethasone-modified adrenal scintiscan, a noninvasive procedure, is described for the preoperative distinction between primary aldosteronism (aldosterone-producing ademona) and idiopathic aldosteronism (bilateral hyperplasia) and for the preoperative localization of aldostersone-producing adenomas. This procedure has been carried out on 17 subsequently proved cases of primary aldosteronism and nine cases (four unexplored) of idiopathic aldosteronism. In the tumor cases, it indicated correctly the side of the tumor in 88 per cent. It was correct in predicting the existence of bilateral hyperplasia in all of the five cases explored. It produced the same response in four more cases believed to have bilateral hyperplasia, in which surgical exploration has not been carried out. Many of the same patients had, in addition, standard adrenal scintiscans (SS), adrenal venography, and determinations of aldosterone in adrenal venous blood. These results are compared with those of the dexamethasone scintiscan (DS). In tumor localization, the 88 per cent figure for the DS was only moderately better than that of the other three (71 per cent, SS; 80 per cent, venography; 80 per cent, adrenal venous aldosterone levels). However, in predicting bilateral hyperplasia, the DS was 100 per cent correct, as were the levels of aldosterone in adrenal venous blood. The SS and adrenal venography failed in bilateral hyperplasia and gave many false-positive results indicating tumor. The DS, a relatively simple outpatient procedure, appears to be at least as effective, both in lateralizing tumors and distinguishing between tumor and bilateral hyperplasia, as the more difficult, expensive, and sometimes hazardous invasive procedure of bilateral adrenal vein catheterization.", "contents": "The dexamethasone-modified adrenal scintiscan in hyporeninemic aldosteronism (tumor versus hyperplasia). A comparison with adrenal venography and adrenal venous aldosterone. The dexamethasone-modified adrenal scintiscan, a noninvasive procedure, is described for the preoperative distinction between primary aldosteronism (aldosterone-producing ademona) and idiopathic aldosteronism (bilateral hyperplasia) and for the preoperative localization of aldostersone-producing adenomas. This procedure has been carried out on 17 subsequently proved cases of primary aldosteronism and nine cases (four unexplored) of idiopathic aldosteronism. In the tumor cases, it indicated correctly the side of the tumor in 88 per cent. It was correct in predicting the existence of bilateral hyperplasia in all of the five cases explored. It produced the same response in four more cases believed to have bilateral hyperplasia, in which surgical exploration has not been carried out. Many of the same patients had, in addition, standard adrenal scintiscans (SS), adrenal venography, and determinations of aldosterone in adrenal venous blood. These results are compared with those of the dexamethasone scintiscan (DS). In tumor localization, the 88 per cent figure for the DS was only moderately better than that of the other three (71 per cent, SS; 80 per cent, venography; 80 per cent, adrenal venous aldosterone levels). However, in predicting bilateral hyperplasia, the DS was 100 per cent correct, as were the levels of aldosterone in adrenal venous blood. The SS and adrenal venography failed in bilateral hyperplasia and gave many false-positive results indicating tumor. The DS, a relatively simple outpatient procedure, appears to be at least as effective, both in lateralizing tumors and distinguishing between tumor and bilateral hyperplasia, as the more difficult, expensive, and sometimes hazardous invasive procedure of bilateral adrenal vein catheterization.", "PMID": 978047}
{"id": "pubmed23n0032_11461", "title": "Measurement of conjugated and unconjugated bilirubin in bile. II. A new thin-layer chromatographic method.", "content": "A new thin-layer chromatographic (TLC) method is described for measurement of conjugated (CB) and unconjugated (UCB) bilirubin in bile. Bile is streaked on a silica-gel plate and developed with chloroform. Bilirubin in the mobile UCB band and stationary CB band is quantitated by diazotization from the scraped gel with p-iodoaniline reagent. Both CB and UCB in bile yielded identical azopigment absorbance. There was no contamination of the UBC band with either CB or oxidative derivatives of bilirubin, and over 98 per cent added 14C-UBC migrated with the mobile band. Recoveries from TLC averaged 98.2 +/- 4.3 per cent of the applied pigment, duplicated agreed within 10 per cent, and the method accurately detected the enzymatic and alkaline hydrolysis of CB to UCB. Concentrations of UCB in nine samples of normal human gallbladder bile were found to be less than 1.9 mg./dl. and never exceeded 1.2 per cent of the total bilirubin.", "contents": "Measurement of conjugated and unconjugated bilirubin in bile. II. A new thin-layer chromatographic method. A new thin-layer chromatographic (TLC) method is described for measurement of conjugated (CB) and unconjugated (UCB) bilirubin in bile. Bile is streaked on a silica-gel plate and developed with chloroform. Bilirubin in the mobile UCB band and stationary CB band is quantitated by diazotization from the scraped gel with p-iodoaniline reagent. Both CB and UCB in bile yielded identical azopigment absorbance. There was no contamination of the UBC band with either CB or oxidative derivatives of bilirubin, and over 98 per cent added 14C-UBC migrated with the mobile band. Recoveries from TLC averaged 98.2 +/- 4.3 per cent of the applied pigment, duplicated agreed within 10 per cent, and the method accurately detected the enzymatic and alkaline hydrolysis of CB to UCB. Concentrations of UCB in nine samples of normal human gallbladder bile were found to be less than 1.9 mg./dl. and never exceeded 1.2 per cent of the total bilirubin.", "PMID": 978048}
{"id": "pubmed23n0032_11462", "title": "Whole blood microculture assay of human lymphocyte function.", "content": "A whole blood microculture assay is described for measuring lymphocyte reactivity to mitogenic and antigenic stimulants. This assay employs heparinized whole blood, serum-free culture medium, microtiter plates, and a Multiple Automated Sample Harvester (MASH). When this assay is compared to other leukocyte assays, its major advantages include (1) the utilization of fewer lymphocytes per microculture, thuus reducing the amount of blood required per test while increasing the number of test agents and replicate cultures which can be employed in any given experiment; (2) the conservation of mitogens, antigens, drugs, enzymes, hormones, lymphokines, and other test agents, some of which are either expensive of difficult to prepare in large quantities; (3) the elimination of lymphocyte isolation and purification procedures which may disrupt the relative proportion of T cells, B cells and antigen-processing cells; and (4) the application of an automated harvester which simplifies and expedites procedures required for processing cells for liquid scintillation counting.", "contents": "Whole blood microculture assay of human lymphocyte function. A whole blood microculture assay is described for measuring lymphocyte reactivity to mitogenic and antigenic stimulants. This assay employs heparinized whole blood, serum-free culture medium, microtiter plates, and a Multiple Automated Sample Harvester (MASH). When this assay is compared to other leukocyte assays, its major advantages include (1) the utilization of fewer lymphocytes per microculture, thuus reducing the amount of blood required per test while increasing the number of test agents and replicate cultures which can be employed in any given experiment; (2) the conservation of mitogens, antigens, drugs, enzymes, hormones, lymphokines, and other test agents, some of which are either expensive of difficult to prepare in large quantities; (3) the elimination of lymphocyte isolation and purification procedures which may disrupt the relative proportion of T cells, B cells and antigen-processing cells; and (4) the application of an automated harvester which simplifies and expedites procedures required for processing cells for liquid scintillation counting.", "PMID": 978049}
{"id": "pubmed23n0032_11463", "title": "Ventricular arrhythmias and cardiac hemodynamics in patients with myocardial infarction. Comparison of the acute and post-hospitalization phases.", "content": "Sixty-six patients with myocardial infarction (MI) were studied during the acute hospital phase and during the six months after hospital discharge. The clinical characteristics, location of infarction, and data from right heart catheterization were studied in an attempt to determine what factors were associated with ventricular rhythm disturbance. Those patients with serious ventricular arrhythmias (SVA) in the acute phase of infarction were found to have a significantly greater degree of myocardial dysfunction as measured by pulmonary artery and pulmonary wedge pressure than patients with more normal rhythm (p less than .05). Clinical classification of patients and location of infarction were not helpful in predicting SVA during the acute infarction period. Knowledge of hemodynamic data, presence of SVA and clinical characteristics in the acute infarction period were of no value in predicting the occurrence of SVA after hospital discharge patients having had an acute diaphragmatic infarction were found to have a higher incidence of SVA after hospital discharge.", "contents": "Ventricular arrhythmias and cardiac hemodynamics in patients with myocardial infarction. Comparison of the acute and post-hospitalization phases. Sixty-six patients with myocardial infarction (MI) were studied during the acute hospital phase and during the six months after hospital discharge. The clinical characteristics, location of infarction, and data from right heart catheterization were studied in an attempt to determine what factors were associated with ventricular rhythm disturbance. Those patients with serious ventricular arrhythmias (SVA) in the acute phase of infarction were found to have a significantly greater degree of myocardial dysfunction as measured by pulmonary artery and pulmonary wedge pressure than patients with more normal rhythm (p less than .05). Clinical classification of patients and location of infarction were not helpful in predicting SVA during the acute infarction period. Knowledge of hemodynamic data, presence of SVA and clinical characteristics in the acute infarction period were of no value in predicting the occurrence of SVA after hospital discharge patients having had an acute diaphragmatic infarction were found to have a higher incidence of SVA after hospital discharge.", "PMID": 978079}
{"id": "pubmed23n0032_11464", "title": "Differences between supine and sitting Frank-lead electrocardiograms.", "content": "Frank-lead electrocardiograms (ECGs) were recorded from 59 adult males with suspected coronary artery disease. Three records were recorded consecutively on frequency modulated tape for each patient in (1) supine position, (2) sitting position with arms relaxed, and (3) sitting position on bicycle with arms on bicycle handles. Electrodes were applied at the level of the fifth intercostal space with patients in the sitting position. Computer measurements of electrocardiographic amplitudes were averaged over ten seconds of each record with these results: (1) QRS spatial amplitudes and R amplitudes in lead z were significantly higher and R amplitudes in lead y lower for sitting than for supine positions. (2) Except for slightly higher R and S amplitudes in lead x for sitting with arms on bicycle, no significant differences were observed between the two sitting positions. (3) These postural differences are significantly greater than those resulting from day-to-day variability of electrode locations. It is hypothesized that electrode level shifts with postural changes are responsible for the observed x- and z-lead changes. For the y-lead changes, it is hypothesized that shifting blood volumes with postural changes are the cause. It is concluded that reference electrocardiographic measurements for stress testing should be obtained from resting ECGs with the patient in the same postural position as that maintained during exercise.", "contents": "Differences between supine and sitting Frank-lead electrocardiograms. Frank-lead electrocardiograms (ECGs) were recorded from 59 adult males with suspected coronary artery disease. Three records were recorded consecutively on frequency modulated tape for each patient in (1) supine position, (2) sitting position with arms relaxed, and (3) sitting position on bicycle with arms on bicycle handles. Electrodes were applied at the level of the fifth intercostal space with patients in the sitting position. Computer measurements of electrocardiographic amplitudes were averaged over ten seconds of each record with these results: (1) QRS spatial amplitudes and R amplitudes in lead z were significantly higher and R amplitudes in lead y lower for sitting than for supine positions. (2) Except for slightly higher R and S amplitudes in lead x for sitting with arms on bicycle, no significant differences were observed between the two sitting positions. (3) These postural differences are significantly greater than those resulting from day-to-day variability of electrode locations. It is hypothesized that electrode level shifts with postural changes are responsible for the observed x- and z-lead changes. For the y-lead changes, it is hypothesized that shifting blood volumes with postural changes are the cause. It is concluded that reference electrocardiographic measurements for stress testing should be obtained from resting ECGs with the patient in the same postural position as that maintained during exercise.", "PMID": 978080}
{"id": "pubmed23n0032_11465", "title": "Polarcardiographic criteria for myocardial infarction in Chinese men.", "content": "Polarcardiograms (PCG) derived from xyz leads of the Frank electrocardiogram (ECG) were recorded in the supine position in 1264 initially healthy middle-aged Chinese men who had been under continuing medical surveillance and were re-examined seven years later. Polarcardiographic criteria for myocardial infarction (MI) were demonstrated in 97 men (7.7%), but only 15 of them showed diagnostic Q waves in the electrocardiogram (ECG). Polarcardiographic criteria for MI were independent of age, but ranged from 4.3% in 464 apparently normal men to 38% in 21 men with manifestations of ischemic heart disease. The possible association of polarcardiographic criteria and a history of smoking was limited to those with clinical evidence of heart disease. The \"false positive\" rate of 4.3% in clinically normal men was similar to that reported in younger Canadians and in Cretan population samples.", "contents": "Polarcardiographic criteria for myocardial infarction in Chinese men. Polarcardiograms (PCG) derived from xyz leads of the Frank electrocardiogram (ECG) were recorded in the supine position in 1264 initially healthy middle-aged Chinese men who had been under continuing medical surveillance and were re-examined seven years later. Polarcardiographic criteria for myocardial infarction (MI) were demonstrated in 97 men (7.7%), but only 15 of them showed diagnostic Q waves in the electrocardiogram (ECG). Polarcardiographic criteria for MI were independent of age, but ranged from 4.3% in 464 apparently normal men to 38% in 21 men with manifestations of ischemic heart disease. The possible association of polarcardiographic criteria and a history of smoking was limited to those with clinical evidence of heart disease. The \"false positive\" rate of 4.3% in clinically normal men was similar to that reported in younger Canadians and in Cretan population samples.", "PMID": 978081}
{"id": "pubmed23n0032_11466", "title": "Precordial ST-segment mapping. 4. Experience with mapping of ST-segment depression in anterior transmural myocardial infarction.", "content": "In order to assess the relative significance of precordial ST-segment elevations and depressions, 32 patients with anterior transmural myocardial infarction were studied utilizing serial 49-lead precordial maps. Theoretically, zones of ST-segment depression adjacent to major zones of ST-segment elevation might represent border areas of mild ischemia, and hence could be more readily amenable to intervention therapy. As expected, an extensive zone of ST-segment elevation was observed precordially in each of these patients. However, zones of ST-segment depression in adjacent areas were noted to occur inconsistently, were limited in distribution and magnitude, and bore no fixed relationship to zones of ST-segment elevation. Thus, mapping of precordial ST-segment depression in anterior transmural infarction probably has a limited role in assessing evolution of ischemic injury or therapy in these patients. This finding does not, however, vitiate the significance of ST-segment depressions in angina, intermediate coronary syndrome, or non-transmural infarction, conditions which may deserve further study using mapping techniques.", "contents": "Precordial ST-segment mapping. 4. Experience with mapping of ST-segment depression in anterior transmural myocardial infarction. In order to assess the relative significance of precordial ST-segment elevations and depressions, 32 patients with anterior transmural myocardial infarction were studied utilizing serial 49-lead precordial maps. Theoretically, zones of ST-segment depression adjacent to major zones of ST-segment elevation might represent border areas of mild ischemia, and hence could be more readily amenable to intervention therapy. As expected, an extensive zone of ST-segment elevation was observed precordially in each of these patients. However, zones of ST-segment depression in adjacent areas were noted to occur inconsistently, were limited in distribution and magnitude, and bore no fixed relationship to zones of ST-segment elevation. Thus, mapping of precordial ST-segment depression in anterior transmural infarction probably has a limited role in assessing evolution of ischemic injury or therapy in these patients. This finding does not, however, vitiate the significance of ST-segment depressions in angina, intermediate coronary syndrome, or non-transmural infarction, conditions which may deserve further study using mapping techniques.", "PMID": 978082}
{"id": "pubmed23n0032_11467", "title": "Precise \"one line\" measurement of intracardiac electrograms.", "content": "Atrioventricular nodal (A-H) and infranodal (H-Q) conduction times were determined in 22 patients undergoing intracardiac electrophysiologic studies. These measurements were determined independently both on line from a digital oscilloscope and from paper recordings at a rate of 100 mm/sec. There was no significant difference and excellent correlation between mean A-H (91.39 +/- 29 versus 95.44 +/- 32 msec) and H-Q (53.48 +/- 14 versus 50.52 +/- 15 msec) intervals determined by these two methods. The digital oscilloscope enables virtually instantaneous highly accurate (+/- 1 msec) timing of intracardiac events and rapid and more efficient processing of data. Potential applications during electrophysiologic studies include precise monitoring of drug effects on atrioventricular conduction and refractoriness, on line analyses of complex rhythm disturbances, and ready storage of data for computer retrieval and analyses.", "contents": "Precise \"one line\" measurement of intracardiac electrograms. Atrioventricular nodal (A-H) and infranodal (H-Q) conduction times were determined in 22 patients undergoing intracardiac electrophysiologic studies. These measurements were determined independently both on line from a digital oscilloscope and from paper recordings at a rate of 100 mm/sec. There was no significant difference and excellent correlation between mean A-H (91.39 +/- 29 versus 95.44 +/- 32 msec) and H-Q (53.48 +/- 14 versus 50.52 +/- 15 msec) intervals determined by these two methods. The digital oscilloscope enables virtually instantaneous highly accurate (+/- 1 msec) timing of intracardiac events and rapid and more efficient processing of data. Potential applications during electrophysiologic studies include precise monitoring of drug effects on atrioventricular conduction and refractoriness, on line analyses of complex rhythm disturbances, and ready storage of data for computer retrieval and analyses.", "PMID": 978083}
{"id": "pubmed23n0032_11468", "title": "The effect of adrenergic enhancement on overdrive excitation.", "content": "The effect of adrenergic enhancement (stimulation of a stellate ganglion or administration of norepinephrine) on arrhythmias induced by ventricular overdrive (\"overdirve excitation\") was studied in dogs with complete atrioventricular block. The following results were obtained. (1) Adrenergic enhancement may lead to abnormal ventricular rhythms. (2) A brief drive during adrenergic enhancement induced or enhanced fast ventricular rhythms. (3) The induced rhythms were characterized by an abrupt onset, a fast rate of discharge, and a moderate degree of slowing before an abrupt cessation. (4) The induced rhythms were accelerated by further drive. (5) Increasing the duration of overdrive during adrenergic enhancement resulted in overdrive suppression and not in overdrive excitation. (6) Prolonged drive could induce a few beats (instead of suppression) but at a rate below control (\"inhibited excitation\"). (7) The arrhythmogenic effects of overdrive and adrenergic enhancement were potentiated by the simultaneous administration of calcium. It is concluded that interventions which increase the inward calcium current (overdrive, adrenergic enhancement and higher [Ca]o) favor the onset and the maintenance of overdrive excitation.", "contents": "The effect of adrenergic enhancement on overdrive excitation. The effect of adrenergic enhancement (stimulation of a stellate ganglion or administration of norepinephrine) on arrhythmias induced by ventricular overdrive (\"overdirve excitation\") was studied in dogs with complete atrioventricular block. The following results were obtained. (1) Adrenergic enhancement may lead to abnormal ventricular rhythms. (2) A brief drive during adrenergic enhancement induced or enhanced fast ventricular rhythms. (3) The induced rhythms were characterized by an abrupt onset, a fast rate of discharge, and a moderate degree of slowing before an abrupt cessation. (4) The induced rhythms were accelerated by further drive. (5) Increasing the duration of overdrive during adrenergic enhancement resulted in overdrive suppression and not in overdrive excitation. (6) Prolonged drive could induce a few beats (instead of suppression) but at a rate below control (\"inhibited excitation\"). (7) The arrhythmogenic effects of overdrive and adrenergic enhancement were potentiated by the simultaneous administration of calcium. It is concluded that interventions which increase the inward calcium current (overdrive, adrenergic enhancement and higher [Ca]o) favor the onset and the maintenance of overdrive excitation.", "PMID": 978084}
{"id": "pubmed23n0032_11469", "title": "The relation of activation boundary size to body surface QRS potential sum.", "content": "The relations of QRS amplitude sums to activation boundary size at two instants was evaluated in five normal subjects. In each subject, QRS amplitude sums from 192 simultaneously recorded torso electrocardiograms and from 35 leads from the precordial area only were obtained 10 and 15 msec after the QRS onset. Activation boundary size at these instants was taken from published studies of ventricular excitation sequence in the human heart. The relative size of electrocargiographically effective boundaries at 10 and 15 msec and the measured QRS amplitude sum at 10 msec were used to predict expected QRS amplitude sums at 15 msec. Differences between the predicted and measured QRS amplitude sums at 15 msec constitute an estimate of error in determining cardiac source size from its expressions in the body surface electrocardiogram (ECG). Results demonstrated a more consistent relation of activation boundary size to QRS sums from the 192 than from the 35 electrode array. Indirectly the results suggest summed ST segment displacement from the larger array of electrodes would be more consistently related to the extent or severity of myocardial injury than an ST segment displacement sum based on 35 precordial leads. Finally, the findings suggested that the absolute sum of an electrocardiographic measurement such as ST segment displacement would provide a better index of lesion size than ST elevation or depression only.", "contents": "The relation of activation boundary size to body surface QRS potential sum. The relations of QRS amplitude sums to activation boundary size at two instants was evaluated in five normal subjects. In each subject, QRS amplitude sums from 192 simultaneously recorded torso electrocardiograms and from 35 leads from the precordial area only were obtained 10 and 15 msec after the QRS onset. Activation boundary size at these instants was taken from published studies of ventricular excitation sequence in the human heart. The relative size of electrocargiographically effective boundaries at 10 and 15 msec and the measured QRS amplitude sum at 10 msec were used to predict expected QRS amplitude sums at 15 msec. Differences between the predicted and measured QRS amplitude sums at 15 msec constitute an estimate of error in determining cardiac source size from its expressions in the body surface electrocardiogram (ECG). Results demonstrated a more consistent relation of activation boundary size to QRS sums from the 192 than from the 35 electrode array. Indirectly the results suggest summed ST segment displacement from the larger array of electrodes would be more consistently related to the extent or severity of myocardial injury than an ST segment displacement sum based on 35 precordial leads. Finally, the findings suggested that the absolute sum of an electrocardiographic measurement such as ST segment displacement would provide a better index of lesion size than ST elevation or depression only.", "PMID": 978085}
{"id": "pubmed23n0032_11470", "title": "Right atrial versus left atrial echo zones: a proposed new criterion for determining the atrial site of retrograde preexcitation.", "content": "In a patient whose electrocardiogram (ECG) initially (1966) showed a Type A Wolff-Parkinson-White pattern, recurrent supraventricular tachycardia (SVT) developed but never subsequently showed antegrade bypass conduction. Intracardiac pacing studies (1975) revealed that premature high right atrial (induced 250-450 msec after atrial depolarization) or coronary sinus depolarization (250-550 msec) resulted in SVT. Late coronary sinus depolarization resulted in SVT without A-H prolongation. During SVT, P wave morphology changed and the coronary sinus atrial electrogram preceded that from the low right atrium; retrograde ventriculoatrial conduction time was 240 msec. Neither pacing the high right atrium or coronary sinus up to rates of 200 beats/min nor progressive atrial premature depolarizations from the high right atrium or coronary sinus resulted in antegrade bypass conduction. Failure of antegrade bypass conduction does not preclude SVT due to retrograde preexcitation and must be distinguished from atrioventricular (A-V) nodal reentry. Atrial effective refractory period (200 msec) was shorter than the minimal time required for an atrial impulse to return to the atrium (380 msec), suggesting concealed antegrade bypass conduction. Stimulation of the atrium linked to the A-V bypass results in earlier bypass activation and recovery and explains the differing high right atrial vs coronary sinus echo zones.", "contents": "Right atrial versus left atrial echo zones: a proposed new criterion for determining the atrial site of retrograde preexcitation. In a patient whose electrocardiogram (ECG) initially (1966) showed a Type A Wolff-Parkinson-White pattern, recurrent supraventricular tachycardia (SVT) developed but never subsequently showed antegrade bypass conduction. Intracardiac pacing studies (1975) revealed that premature high right atrial (induced 250-450 msec after atrial depolarization) or coronary sinus depolarization (250-550 msec) resulted in SVT. Late coronary sinus depolarization resulted in SVT without A-H prolongation. During SVT, P wave morphology changed and the coronary sinus atrial electrogram preceded that from the low right atrium; retrograde ventriculoatrial conduction time was 240 msec. Neither pacing the high right atrium or coronary sinus up to rates of 200 beats/min nor progressive atrial premature depolarizations from the high right atrium or coronary sinus resulted in antegrade bypass conduction. Failure of antegrade bypass conduction does not preclude SVT due to retrograde preexcitation and must be distinguished from atrioventricular (A-V) nodal reentry. Atrial effective refractory period (200 msec) was shorter than the minimal time required for an atrial impulse to return to the atrium (380 msec), suggesting concealed antegrade bypass conduction. Stimulation of the atrium linked to the A-V bypass results in earlier bypass activation and recovery and explains the differing high right atrial vs coronary sinus echo zones.", "PMID": 978086}
{"id": "pubmed23n0032_11471", "title": "An unusual mechanism of arrhythmia production.", "content": "An unusual electrocardiogram (ECG) is presented which demonstrates two mechanisms of communication between the atria and the ventricles. The first appears to reflect ordinary atrio-ventricular impulse propagation. The second appears likely to represent stimulation of the ventricles by mechanical atrial systole.", "contents": "An unusual mechanism of arrhythmia production. An unusual electrocardiogram (ECG) is presented which demonstrates two mechanisms of communication between the atria and the ventricles. The first appears to reflect ordinary atrio-ventricular impulse propagation. The second appears likely to represent stimulation of the ventricles by mechanical atrial systole.", "PMID": 978087}
{"id": "pubmed23n0032_11472", "title": "Diagnosis of high anterolateral and true posterior myocardial infarction by chest wall ECG-mapping.", "content": "Precordial and posterior chest wall ST-mapping has been utilized in a patient with high anterolateral and true posterior myocardial infarction. In this patient evolution of the anterolateral component of the infarct was accurately delineated by changes in the precordial map which were not detected in the standard precordial leads. The true posterior component of the infarct was clearly diagnosed by detection of pathological Q waves and ST elevations in posterior chest wall maps, whereas right precordial standard leads had shown only suggestive reciprocal changes. The study illustrates the superiority of localized chest wall mapping over conventional 12-lead electrocardiography in detecting transmural infarction in high anterolateral and posterior regions of the left ventricle.", "contents": "Diagnosis of high anterolateral and true posterior myocardial infarction by chest wall ECG-mapping. Precordial and posterior chest wall ST-mapping has been utilized in a patient with high anterolateral and true posterior myocardial infarction. In this patient evolution of the anterolateral component of the infarct was accurately delineated by changes in the precordial map which were not detected in the standard precordial leads. The true posterior component of the infarct was clearly diagnosed by detection of pathological Q waves and ST elevations in posterior chest wall maps, whereas right precordial standard leads had shown only suggestive reciprocal changes. The study illustrates the superiority of localized chest wall mapping over conventional 12-lead electrocardiography in detecting transmural infarction in high anterolateral and posterior regions of the left ventricle.", "PMID": 978088}
{"id": "pubmed23n0032_11473", "title": "Idioventricular parasystolic rhythms.", "content": "A description is given of the electrocardiograms of three subjects with artificial demand pacemakers in whom no inhibition of the idioventricular rhythms occurs, thus causing unusual relationships between the artificial and the spontaneous rhythms. The latter are protected and therefore appear to be parasystolic. In the first two cases, interpolated artificial beats are inscribed in the spontaneous rhythm. In the third case, the spontaneous parasystole is intermittent and alternates with concealed extrasystolic beats originating in the same focus.", "contents": "Idioventricular parasystolic rhythms. A description is given of the electrocardiograms of three subjects with artificial demand pacemakers in whom no inhibition of the idioventricular rhythms occurs, thus causing unusual relationships between the artificial and the spontaneous rhythms. The latter are protected and therefore appear to be parasystolic. In the first two cases, interpolated artificial beats are inscribed in the spontaneous rhythm. In the third case, the spontaneous parasystole is intermittent and alternates with concealed extrasystolic beats originating in the same focus.", "PMID": 978089}
{"id": "pubmed23n0032_11474", "title": "Primary ST and T wave abnormalities in the diagnosis of acute anterior myocardial infarction during permanent ventricular pacing.", "content": "The electrocardiographic diagnosis of myocardial infarction in patients with ventricular pacemakers is often difficult. This report describes the electrocardiograms of two patients with permanent ventricular pacemakers in whom the diagnosis of acute anterior myocardial infarction could be made with confidence by observing the evolution of primary ST and T wave abnormalities in the absence of specific QRS changes.", "contents": "Primary ST and T wave abnormalities in the diagnosis of acute anterior myocardial infarction during permanent ventricular pacing. The electrocardiographic diagnosis of myocardial infarction in patients with ventricular pacemakers is often difficult. This report describes the electrocardiograms of two patients with permanent ventricular pacemakers in whom the diagnosis of acute anterior myocardial infarction could be made with confidence by observing the evolution of primary ST and T wave abnormalities in the absence of specific QRS changes.", "PMID": 978091}
{"id": "pubmed23n0032_11475", "title": "Early evaluation of a rechargeable pacemaker system.", "content": "A rechargeable demand pulse generator for permanent transvenous cardiac pacing was evaluated in 66 patients. During a cumulative follow-up period of 895 patient months there was no instance of failure of either the pulse generator or of the recharging circuit. Acceptance of the recharging concept was high, there being only one patient in whom it was necessary to replace the rechargeable generator because of inability to master the recharging technique. The early findings indicate that with proper patient selection the rechargeable pulse generator promises to be an important contribution to pacemaker therapy.", "contents": "Early evaluation of a rechargeable pacemaker system. A rechargeable demand pulse generator for permanent transvenous cardiac pacing was evaluated in 66 patients. During a cumulative follow-up period of 895 patient months there was no instance of failure of either the pulse generator or of the recharging circuit. Acceptance of the recharging concept was high, there being only one patient in whom it was necessary to replace the rechargeable generator because of inability to master the recharging technique. The early findings indicate that with proper patient selection the rechargeable pulse generator promises to be an important contribution to pacemaker therapy.", "PMID": 978092}
{"id": "pubmed23n0032_11476", "title": "Part II: magnetic field produced by a current dipole.", "content": "To understand the MCG, electrical models of the heart must be used in which the basic building-block is usually the current dipole. The dipole's magnetic field is generally made up of two parts: 1. the contribution by the dipole element itself, which is mathematically simple; 2. the contribution by the current generated in the volume conductor by the dipole, which is complicated and depends on the boundaries; for special boundaries this contribution is zero to Bz, the component of magnetic field which is normal to the boundary. This applies to the boundaries of the semi-infinite volume conductor, the infinite slab, and the sphere. This property allows great simplification in solving the magnetic forward and inverse problems. Because of its importance, it is proven with electrolytic tank experiments. Based on this property, a method is presented for estimating the presence of those dipole combinations which produce a suppressed surface potential; it consists of a visual examination of an \"arrow\" display of Bz.", "contents": "Part II: magnetic field produced by a current dipole. To understand the MCG, electrical models of the heart must be used in which the basic building-block is usually the current dipole. The dipole's magnetic field is generally made up of two parts: 1. the contribution by the dipole element itself, which is mathematically simple; 2. the contribution by the current generated in the volume conductor by the dipole, which is complicated and depends on the boundaries; for special boundaries this contribution is zero to Bz, the component of magnetic field which is normal to the boundary. This applies to the boundaries of the semi-infinite volume conductor, the infinite slab, and the sphere. This property allows great simplification in solving the magnetic forward and inverse problems. Because of its importance, it is proven with electrolytic tank experiments. Based on this property, a method is presented for estimating the presence of those dipole combinations which produce a suppressed surface potential; it consists of a visual examination of an \"arrow\" display of Bz.", "PMID": 978094}
{"id": "pubmed23n0032_11477", "title": "Part III: the effect of the torso boundaries on the magnetocardiogram.", "content": "The magnetic field produced by a current dipole is made up of two parts: the field from the dipole element, and from the current generated by the dipole in the volume conductor. It was previously shown for the semi-infinite volume conductor, infinite slab, and the sphere that the volume-current contribution is zero to the component of magnetic field which is normal to the boundary. The volume conductor in the form of the human torso is here investigated by computer simulation. Three different heart-torso models are used. The contribution to the normal field component (Bn) by the volume current (via the boundaries) and by the heart dipoles are computed. For comparison, the boundary contribution to the surface potential (V) is also computed. For Bn the three models yield a ratio of boundary to dipole contribution in the same range, with 0.28 as the average. Simple subtractions can make this ratio negligible. For V the equivalent ratio is somewhat greater. The arrow map, developed previously to display Bn over special surfaces, is shown to be valid for the human torso, for visually estimating the heart dipoles.", "contents": "Part III: the effect of the torso boundaries on the magnetocardiogram. The magnetic field produced by a current dipole is made up of two parts: the field from the dipole element, and from the current generated by the dipole in the volume conductor. It was previously shown for the semi-infinite volume conductor, infinite slab, and the sphere that the volume-current contribution is zero to the component of magnetic field which is normal to the boundary. The volume conductor in the form of the human torso is here investigated by computer simulation. Three different heart-torso models are used. The contribution to the normal field component (Bn) by the volume current (via the boundaries) and by the heart dipoles are computed. For comparison, the boundary contribution to the surface potential (V) is also computed. For Bn the three models yield a ratio of boundary to dipole contribution in the same range, with 0.28 as the average. Simple subtractions can make this ratio negligible. For V the equivalent ratio is somewhat greater. The arrow map, developed previously to display Bn over special surfaces, is shown to be valid for the human torso, for visually estimating the heart dipoles.", "PMID": 978095}
{"id": "pubmed23n0032_11478", "title": "Part IV: visual determination of generators of the magnetocardiogram.", "content": "Previously it was shown that Bn, the component of the heart's magnetic field which is normal to the chest, is in large part produced directly by the generators in the heart; the volume-current (or boundary) contribution to Bn is relatively small. It was also shown that the distribution of Bn across the chest could be displayed as an arrow map which visually reveals the underlying dipole sources, in particular those dipole combinations which produce suppresed surface potentials but undiminished Bn. Arrow maps of two normal and one abnormal heart subject are here presented. The purpose is to demonstrate that the generators of the MCG can be coarsely estimated by visual inspection of these maps. The generators of the normal hearts are visually determined by comparing their arrow maps with those computed from the Cuffin-Selvester model. The generators of the abnormal heart are visually determined by trial-and-error, with the aid of a simple computing program. These examples are the first determinations, however approximate, of multi-dipole generators of the actual MCGs. Some possible new information is indicated.", "contents": "Part IV: visual determination of generators of the magnetocardiogram. Previously it was shown that Bn, the component of the heart's magnetic field which is normal to the chest, is in large part produced directly by the generators in the heart; the volume-current (or boundary) contribution to Bn is relatively small. It was also shown that the distribution of Bn across the chest could be displayed as an arrow map which visually reveals the underlying dipole sources, in particular those dipole combinations which produce suppresed surface potentials but undiminished Bn. Arrow maps of two normal and one abnormal heart subject are here presented. The purpose is to demonstrate that the generators of the MCG can be coarsely estimated by visual inspection of these maps. The generators of the normal hearts are visually determined by comparing their arrow maps with those computed from the Cuffin-Selvester model. The generators of the abnormal heart are visually determined by trial-and-error, with the aid of a simple computing program. These examples are the first determinations, however approximate, of multi-dipole generators of the actual MCGs. Some possible new information is indicated.", "PMID": 978096}
{"id": "pubmed23n0032_11479", "title": "Production of antisera against highly purified human follicle-stimulating hormone, luteinizing hormone and thyroid-stimulating hormone.", "content": "Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10,000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7-5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the beta-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.", "contents": "Production of antisera against highly purified human follicle-stimulating hormone, luteinizing hormone and thyroid-stimulating hormone. Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10,000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7-5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the beta-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.", "PMID": 978097}
{"id": "pubmed23n0032_11480", "title": "Serum gonadotrophin levels during development in male, female and androgenized female rats and the effect of general disturbance on high luteinizing hormone levels.", "content": "Serum LH, FSH and prolactin levels were measured in blood samples which were obtained by decapitation from groups of female, neonatally androgenized female and male Wistar rats at 2-day intervals from birth to maturity. An increase in serum FSH levels was observed between 4 and 24 days of age in both the female and androgenized female groups, while a much later increase, between 28 and 44 days of age, occurred in the males. Serum prolactin contrast, serum LH levels were in general low in all three groups of animals, although very high levels (greater than 7 ng/ml) were recorded in 22 out of 168 females and 8 out of 192 males between 4 and 28 days of age, as well as in adult males; occasional high LH levels were also seen in the androgenized females. The nature of the high serum LH levels was investigated in anaesthetized and unanaesthetized immature females by serial blood sampling using a number of techniques. Unexpectedly, only three out of 58 animals had high LH levels: two of these showed an episodic form of LH release during which levels increased to peak values and then declined within a period of about 30 min. On investigation it was found that general disturbance within the 45 min before decapitation could inhibit high LH levels in females aged between 23 and 30 days.", "contents": "Serum gonadotrophin levels during development in male, female and androgenized female rats and the effect of general disturbance on high luteinizing hormone levels. Serum LH, FSH and prolactin levels were measured in blood samples which were obtained by decapitation from groups of female, neonatally androgenized female and male Wistar rats at 2-day intervals from birth to maturity. An increase in serum FSH levels was observed between 4 and 24 days of age in both the female and androgenized female groups, while a much later increase, between 28 and 44 days of age, occurred in the males. Serum prolactin contrast, serum LH levels were in general low in all three groups of animals, although very high levels (greater than 7 ng/ml) were recorded in 22 out of 168 females and 8 out of 192 males between 4 and 28 days of age, as well as in adult males; occasional high LH levels were also seen in the androgenized females. The nature of the high serum LH levels was investigated in anaesthetized and unanaesthetized immature females by serial blood sampling using a number of techniques. Unexpectedly, only three out of 58 animals had high LH levels: two of these showed an episodic form of LH release during which levels increased to peak values and then declined within a period of about 30 min. On investigation it was found that general disturbance within the 45 min before decapitation could inhibit high LH levels in females aged between 23 and 30 days.", "PMID": 978098}
{"id": "pubmed23n0032_11481", "title": "Comparison between the binding of 19-nortestosterone, 5alpha-dihydrotestosterone and testosterone in rat prostate and bulbocavernosus/levator ani muscle.", "content": "Specific binding of (3H)19-nortestosterone in the 100000 g cytosol of the rat bulbocavernosus/levator ani muscle (BCLA) and prostate was demonstrated by agargel electrophoresis at low temperature and compared qualitatively and quantitatively with the binding of tritiated testosterone and 5alpha-dihydrotestosterone (5alpha-DHT). Both tissues showed a greater binding affinity for 5alpha-DHT than for 19-nortestosterone, with testosterone binding the least well of the three. The relative binding affinities in the BCLA and prostate were: 19-nortestosterone:testosterone=1-4, 1.-nortestosterone: 5alpha-DHT = 0.7 The differences were statistically significant (P less than 0-02). The concentrations of receptor sites for 5alpha-DHT were 171 +/- 20 (S.D.) fmol/mg prostatic cytosol protein and 24 +/- 4 (S.D.) fmol/mg BCLA cytosol protein. The in-vitro metabolism of the three steroids in both tissues was aldo investigated by thin-layer chromatography. After incubating for 2 h at 0 degrees C the prostate was shown to reduce 26% of the 5alpha-DHT to androstanediols whilst the BCLA showed a 5% conversion. Testosterone was converted by the prostate to 5alpha-DHT (10%) and the androstanediols (6%) whilst the BCLA showed little activity in this respect. Comparing these in-vitro data with in-vivo findings from the literature, in both organs there is a positive correlation of the extent of binding in vitro to the stimulation of growth in vivo, bearing in mind that testosterone is metabolized to 5alpha-DHT in the prostate whilst in the BCLA, 5alpha-reductase is essentially absent.", "contents": "Comparison between the binding of 19-nortestosterone, 5alpha-dihydrotestosterone and testosterone in rat prostate and bulbocavernosus/levator ani muscle. Specific binding of (3H)19-nortestosterone in the 100000 g cytosol of the rat bulbocavernosus/levator ani muscle (BCLA) and prostate was demonstrated by agargel electrophoresis at low temperature and compared qualitatively and quantitatively with the binding of tritiated testosterone and 5alpha-dihydrotestosterone (5alpha-DHT). Both tissues showed a greater binding affinity for 5alpha-DHT than for 19-nortestosterone, with testosterone binding the least well of the three. The relative binding affinities in the BCLA and prostate were: 19-nortestosterone:testosterone=1-4, 1.-nortestosterone: 5alpha-DHT = 0.7 The differences were statistically significant (P less than 0-02). The concentrations of receptor sites for 5alpha-DHT were 171 +/- 20 (S.D.) fmol/mg prostatic cytosol protein and 24 +/- 4 (S.D.) fmol/mg BCLA cytosol protein. The in-vitro metabolism of the three steroids in both tissues was aldo investigated by thin-layer chromatography. After incubating for 2 h at 0 degrees C the prostate was shown to reduce 26% of the 5alpha-DHT to androstanediols whilst the BCLA showed a 5% conversion. Testosterone was converted by the prostate to 5alpha-DHT (10%) and the androstanediols (6%) whilst the BCLA showed little activity in this respect. Comparing these in-vitro data with in-vivo findings from the literature, in both organs there is a positive correlation of the extent of binding in vitro to the stimulation of growth in vivo, bearing in mind that testosterone is metabolized to 5alpha-DHT in the prostate whilst in the BCLA, 5alpha-reductase is essentially absent.", "PMID": 978099}
{"id": "pubmed23n0032_11482", "title": "Mammary nucleic acids and pituitary prolactin secretion during prolonged lactation in mice.", "content": "Lactation was prolonged until 61 days by repeated renewal of litters every week after day 12 in primiparous C3H/He strain mice. On days 12, 19, 40, and 61 of lactation, litters were removed for 5 h and after 1 h of resuckling the synthesis of DNA and RNA in the mammary gland was estimated by the incorporation of (3H)thymidine and (14C)uridine into mammary DNA and RNA in vitro respectively. Mammary nucleic acid content and pituitary and plasma levels of prolactin were also assayed. Nulliparous mice were similarly treated on day 19 of pregnancy. The percentage gain in litter weight per week was highest between days 5 and 12 of lactation, declined until days 26-33 and became steady thereafter. Mammary DNA synthesis was extremely high on day 19 of pregnancy, decreased on day 12 of lactation to less than one-fifteenth of that on day 19 of pregnancy and increased linearly therafter. Changes in mammary DNA content were not so marked, but DNA content was high on days 12 and 19 of lactation. RNA synthesis was highest on day 19 of pregnancy, abruptly decreased on days 12 and 19 of lactation and increased again with the advance of lactation. Mammary RNA content, RNA:DNA and 14C:3H ratios increased from day 19 of pregnancy to days 12-19 of lactation and decreased on days 40 and 61. While the pituitary levels of prolactin were almost constant during lactation, they were significantly higher than those on day 19 of pregnancy. There were only slight differences in plasma prolactin levels at any stage.", "contents": "Mammary nucleic acids and pituitary prolactin secretion during prolonged lactation in mice. Lactation was prolonged until 61 days by repeated renewal of litters every week after day 12 in primiparous C3H/He strain mice. On days 12, 19, 40, and 61 of lactation, litters were removed for 5 h and after 1 h of resuckling the synthesis of DNA and RNA in the mammary gland was estimated by the incorporation of (3H)thymidine and (14C)uridine into mammary DNA and RNA in vitro respectively. Mammary nucleic acid content and pituitary and plasma levels of prolactin were also assayed. Nulliparous mice were similarly treated on day 19 of pregnancy. The percentage gain in litter weight per week was highest between days 5 and 12 of lactation, declined until days 26-33 and became steady thereafter. Mammary DNA synthesis was extremely high on day 19 of pregnancy, decreased on day 12 of lactation to less than one-fifteenth of that on day 19 of pregnancy and increased linearly therafter. Changes in mammary DNA content were not so marked, but DNA content was high on days 12 and 19 of lactation. RNA synthesis was highest on day 19 of pregnancy, abruptly decreased on days 12 and 19 of lactation and increased again with the advance of lactation. Mammary RNA content, RNA:DNA and 14C:3H ratios increased from day 19 of pregnancy to days 12-19 of lactation and decreased on days 40 and 61. While the pituitary levels of prolactin were almost constant during lactation, they were significantly higher than those on day 19 of pregnancy. There were only slight differences in plasma prolactin levels at any stage.", "PMID": 978100}
{"id": "pubmed23n0032_11483", "title": "Effects of oestradiol-18beta, hypophysectomy and age on cytoplasmic oestradiol-17beta receptor sites in rat testis interstitial tissue.", "content": "After administration of oestradiol-17beta to intact mature and immature rats, a decrease in the testicular concentration of specific oestradiol-binding sites was observed within 1 h. The binding capacity was replenished starting about 3 h after oestradiol administration and after 5 h the oestrogen receptor level had returned to control values. Exposure of intact animals to oestradiol-17beta for longer periods (up to 24 h) did not result in an increase of receptor levels in testicular cytosol. Mature animals which were hypophysectomized for periods of up to 10 days did not show a significant change in the number of specific oestradiol-binding sites in either total testicular tissue or dissected interstitial tissue. At 15 days or longer periods after hypophysectomy, an apparent increase in receptor concentrations in total testicular cytosol was observed due to a relative increase in the amount of interstitial tissue. A specific oestradiol-binding protein is present in plasma of immature male rats aged less than 30 days. This plasma protein could also be demonstrated in the cytosol of testes of immature rats. In contrast to the cytosol receptor, which shows a moderate affinity for diethylstilbestrol (DES), the plasma protein did not bind DES. The sedimentation values of the plasma protein and the oestradiol receptor were 4 S and 8 S respectively. These differences in characteristics made it possible to demonstrate the presence of the oestradiol receptor in addition to the binding protein in testicular cytosol of rats from 14 days of age onwards. The nuclear receptor for oestradiol-17beta could be demonstrated after incubation of testicular tissue of rats from 4 days of age onwards.", "contents": "Effects of oestradiol-18beta, hypophysectomy and age on cytoplasmic oestradiol-17beta receptor sites in rat testis interstitial tissue. After administration of oestradiol-17beta to intact mature and immature rats, a decrease in the testicular concentration of specific oestradiol-binding sites was observed within 1 h. The binding capacity was replenished starting about 3 h after oestradiol administration and after 5 h the oestrogen receptor level had returned to control values. Exposure of intact animals to oestradiol-17beta for longer periods (up to 24 h) did not result in an increase of receptor levels in testicular cytosol. Mature animals which were hypophysectomized for periods of up to 10 days did not show a significant change in the number of specific oestradiol-binding sites in either total testicular tissue or dissected interstitial tissue. At 15 days or longer periods after hypophysectomy, an apparent increase in receptor concentrations in total testicular cytosol was observed due to a relative increase in the amount of interstitial tissue. A specific oestradiol-binding protein is present in plasma of immature male rats aged less than 30 days. This plasma protein could also be demonstrated in the cytosol of testes of immature rats. In contrast to the cytosol receptor, which shows a moderate affinity for diethylstilbestrol (DES), the plasma protein did not bind DES. The sedimentation values of the plasma protein and the oestradiol receptor were 4 S and 8 S respectively. These differences in characteristics made it possible to demonstrate the presence of the oestradiol receptor in addition to the binding protein in testicular cytosol of rats from 14 days of age onwards. The nuclear receptor for oestradiol-17beta could be demonstrated after incubation of testicular tissue of rats from 4 days of age onwards.", "PMID": 978101}
{"id": "pubmed23n0032_11484", "title": "Lordosis behaviour in male, female and androgenized female rats.", "content": "Sex differences in the lordodis response of adult rats to ovarian hormones were studied in a series of experiments. Male rats were less sensitive to oestradiol benzoate (OB, a single injection of 10, 100 or 1000 mug/kg or seven daily injections of 2, 10, or 50mglkg)then were female rats.  Oestradiol benzoate-primed (10 mglkg)female, but not male, rats showed dose-dependent responses to progesterone (0-4, 2-0 or 10-0 mg/kg/. male rats responded clearly to progesterone (2 mg/rat) only when primed with a high dose of OB (100 mug/rat). Display of the whole pattern of female sexual behaviour was induced in male rats by treatment with 100 mug OB and 2 mg progesterone. Female rats treated with 1 mg testosterone propionate (TP) on day 4 of life, ovariectomized as adults and tested under the same endocrine conditions as the rats described above, retained behavioural OB sensitivity but responded poorly to progesterone. Evidence is presented that ovarian secretions during development significantly modify the response of neonatally TP-treated and normal female rats to OB in adulthood.", "contents": "Lordosis behaviour in male, female and androgenized female rats. Sex differences in the lordodis response of adult rats to ovarian hormones were studied in a series of experiments. Male rats were less sensitive to oestradiol benzoate (OB, a single injection of 10, 100 or 1000 mug/kg or seven daily injections of 2, 10, or 50mglkg)then were female rats.  Oestradiol benzoate-primed (10 mglkg)female, but not male, rats showed dose-dependent responses to progesterone (0-4, 2-0 or 10-0 mg/kg/. male rats responded clearly to progesterone (2 mg/rat) only when primed with a high dose of OB (100 mug/rat). Display of the whole pattern of female sexual behaviour was induced in male rats by treatment with 100 mug OB and 2 mg progesterone. Female rats treated with 1 mg testosterone propionate (TP) on day 4 of life, ovariectomized as adults and tested under the same endocrine conditions as the rats described above, retained behavioural OB sensitivity but responded poorly to progesterone. Evidence is presented that ovarian secretions during development significantly modify the response of neonatally TP-treated and normal female rats to OB in adulthood.", "PMID": 978102}
{"id": "pubmed23n0032_11485", "title": "Effect of the anti-oestrogen tamoxifen on plasma levels of luteinizing hormone, follicle-stimulating hormone, prolactin, oestradiol and progesterone in normal pre-menopausal women.", "content": "Plasma levels of LH, FSH, prolactin, oestradiol and progesterone were determined daily during two consecutive menstrual cycles in six women volunteers. During the first (control) cycle no treatment was given and normal secretion of these hormones was observed. Oral administration of tamoxifen (20 mg/day), for either 5 or 10 days of the follicular phase of the second cycle, caused no change in either the overall length of the cycle or the time of occurrence of the mid-cycle gonadotrophin surge. There was little difference in the secretion of LH, FSH and progesterone during the control and test cycles. A two- to eight-fold increase in oestradiol levels was observed during test cycle which was most pronounced at the times of mid-cycle and mid-luteal hormone peaks. There was a significant decrease in plasma prolactin levels at mid-cycle but no real difference could be seen during the remainder of the cycle. The data suggest that tamoxifen may act directly on the ovary to stimulate oestradiol release without intermediary gonadotrophin stimulation. As the drug apparently inhibited prolactin secretion even in the presence of high oestradiol levels, an alternative explanation may be that the reduced prolactin concentration permits augmented ovarian stimulation by normal concentrations of gonadotrophins.", "contents": "Effect of the anti-oestrogen tamoxifen on plasma levels of luteinizing hormone, follicle-stimulating hormone, prolactin, oestradiol and progesterone in normal pre-menopausal women. Plasma levels of LH, FSH, prolactin, oestradiol and progesterone were determined daily during two consecutive menstrual cycles in six women volunteers. During the first (control) cycle no treatment was given and normal secretion of these hormones was observed. Oral administration of tamoxifen (20 mg/day), for either 5 or 10 days of the follicular phase of the second cycle, caused no change in either the overall length of the cycle or the time of occurrence of the mid-cycle gonadotrophin surge. There was little difference in the secretion of LH, FSH and progesterone during the control and test cycles. A two- to eight-fold increase in oestradiol levels was observed during test cycle which was most pronounced at the times of mid-cycle and mid-luteal hormone peaks. There was a significant decrease in plasma prolactin levels at mid-cycle but no real difference could be seen during the remainder of the cycle. The data suggest that tamoxifen may act directly on the ovary to stimulate oestradiol release without intermediary gonadotrophin stimulation. As the drug apparently inhibited prolactin secretion even in the presence of high oestradiol levels, an alternative explanation may be that the reduced prolactin concentration permits augmented ovarian stimulation by normal concentrations of gonadotrophins.", "PMID": 978103}
{"id": "pubmed23n0032_11486", "title": "Selection for high and low adrenal weight in mice.", "content": "Four strains of mice were used to found a heterogenous stock from which one line was selected for high adrenal weight/100 g body weight. By the eighth generation of selection, two stocks of mice were produced, one with adrenals whose absolute weight was greater than any of the parental strains. The realized heritability estimates were 75% for females and 67% for males during the period of selection, showing that a large component of variation in adrenal weight was genetic in origin. Asymmetry in response to selection was also noticed and attributed to possible variation in adrenal structure between sexes. Estimates of variances in each generation, particularly the eighth, did not show any decline which suggested that further responses to selection would be possible with further selection.", "contents": "Selection for high and low adrenal weight in mice. Four strains of mice were used to found a heterogenous stock from which one line was selected for high adrenal weight/100 g body weight. By the eighth generation of selection, two stocks of mice were produced, one with adrenals whose absolute weight was greater than any of the parental strains. The realized heritability estimates were 75% for females and 67% for males during the period of selection, showing that a large component of variation in adrenal weight was genetic in origin. Asymmetry in response to selection was also noticed and attributed to possible variation in adrenal structure between sexes. Estimates of variances in each generation, particularly the eighth, did not show any decline which suggested that further responses to selection would be possible with further selection.", "PMID": 978104}
{"id": "pubmed23n0032_11487", "title": "Serum follicle-stimulating hormone, luteinizing hormone and progesterone concentrations in pseudopregnant rats treated with medroxyprogesterone acetate.", "content": "Pseudopregnant rats were treated early in pseudopregnancy with 1 or 10 mg medroxyprogesterone acetate (MPA). Serum FSH, LH and progesterone concentrations were determined on days 2-20 of pseudopregnancy in treated and control rats. The mean duration of pseudopregnancy was 13-5 days in the control animals, but when animals were treated with 1 mg MPA a dioestrous period of 21-4 days was observed. A period with leucocytic vaginal smears of at least 2 months was observed after treatment with 10 mg MPA. Injection with MPA on day 3 of pseudopregnancy did not affect the serum FSH concentrations during the subsequent days. The progesterone pattern was alike in the three groups of animals, i.e. the duration of the activity of the corpora lutea was similar in all groups. However, 10 mg MPA slightly lowered progesterone concentrations on days 4-8 of pseudopregnancy. In the saline-treated rats, LH concentrations decreased from days 2-5, and remained low until they increased after day 11 of pseudopregnancy. This increase was delayed until day 20 in the animals treated with 1 mg MPA, and was not observed in the animals treated with 10 mg MPA. It is argued that the increase of LH concentration at the end of pseudopregnency is not instrumental in the decrease of peripheral progesterone concentration but rather that the decrease in the progesterone concentration leads to the increase in the LH concentration.", "contents": "Serum follicle-stimulating hormone, luteinizing hormone and progesterone concentrations in pseudopregnant rats treated with medroxyprogesterone acetate. Pseudopregnant rats were treated early in pseudopregnancy with 1 or 10 mg medroxyprogesterone acetate (MPA). Serum FSH, LH and progesterone concentrations were determined on days 2-20 of pseudopregnancy in treated and control rats. The mean duration of pseudopregnancy was 13-5 days in the control animals, but when animals were treated with 1 mg MPA a dioestrous period of 21-4 days was observed. A period with leucocytic vaginal smears of at least 2 months was observed after treatment with 10 mg MPA. Injection with MPA on day 3 of pseudopregnancy did not affect the serum FSH concentrations during the subsequent days. The progesterone pattern was alike in the three groups of animals, i.e. the duration of the activity of the corpora lutea was similar in all groups. However, 10 mg MPA slightly lowered progesterone concentrations on days 4-8 of pseudopregnancy. In the saline-treated rats, LH concentrations decreased from days 2-5, and remained low until they increased after day 11 of pseudopregnancy. This increase was delayed until day 20 in the animals treated with 1 mg MPA, and was not observed in the animals treated with 10 mg MPA. It is argued that the increase of LH concentration at the end of pseudopregnency is not instrumental in the decrease of peripheral progesterone concentration but rather that the decrease in the progesterone concentration leads to the increase in the LH concentration.", "PMID": 978105}
{"id": "pubmed23n0032_11488", "title": "Binding of human growth hormone to hepatic lactogenic binding sites: regulation by oestrogens and androgens.", "content": "The binding of 125I-labelled human growth hormone (HGH) to the 'lactogenic' binding sites of rat liver membranes has been shown to be highly dependent on the oestrogen and androgen status of the membranes has been shown to be highly dependent on the oestrogen and androgen status of the animal from which the membranes were prepared. Oestradiol treatment of either male or female rats induced a highly significant rise in HGH binding. The minimum effective dose used was 2-5 mug/day and the rise in HGH binding was apparent after 4 days of treatment. Folowing cessation of oestradiol treatment of male rats HGH binding declined with a half-time of approximately 9 days. In contrast to the stimulatory effect of oestrogen, treatment of female rats with testosterone propionate (minimum effective dose 100-200 mug/day) led to a marked reduction in HGH binding. The influence of both oestrogens and androgens was confirmed following the removal of endogenous sex steroids by adrenalectomy-ovariectomy of female rats and castration of male rats. Scratchard analysis showed that, with the possible exception of adrenalectomy-ovariectomy, all pharmacologically and physiologically induced changes in HGH specific binding reflected changes in binding site capacity; there were no changes in binding affinity. While earlier studies have indicated that the oestrogen effect is primarily indirect and is mediated by the pituitary gland, the mode of action of the androgens is currently unknown. The relatively slow response of HGH binding to hormonal changes would support an indirect action for both the sex steroids. The stimulatory effect of oestrogens and the inhibitory effect of androgens may provide an explanation for the marked sex difference in HGH binding to rat liver membranes.", "contents": "Binding of human growth hormone to hepatic lactogenic binding sites: regulation by oestrogens and androgens. The binding of 125I-labelled human growth hormone (HGH) to the 'lactogenic' binding sites of rat liver membranes has been shown to be highly dependent on the oestrogen and androgen status of the membranes has been shown to be highly dependent on the oestrogen and androgen status of the animal from which the membranes were prepared. Oestradiol treatment of either male or female rats induced a highly significant rise in HGH binding. The minimum effective dose used was 2-5 mug/day and the rise in HGH binding was apparent after 4 days of treatment. Folowing cessation of oestradiol treatment of male rats HGH binding declined with a half-time of approximately 9 days. In contrast to the stimulatory effect of oestrogen, treatment of female rats with testosterone propionate (minimum effective dose 100-200 mug/day) led to a marked reduction in HGH binding. The influence of both oestrogens and androgens was confirmed following the removal of endogenous sex steroids by adrenalectomy-ovariectomy of female rats and castration of male rats. Scratchard analysis showed that, with the possible exception of adrenalectomy-ovariectomy, all pharmacologically and physiologically induced changes in HGH specific binding reflected changes in binding site capacity; there were no changes in binding affinity. While earlier studies have indicated that the oestrogen effect is primarily indirect and is mediated by the pituitary gland, the mode of action of the androgens is currently unknown. The relatively slow response of HGH binding to hormonal changes would support an indirect action for both the sex steroids. The stimulatory effect of oestrogens and the inhibitory effect of androgens may provide an explanation for the marked sex difference in HGH binding to rat liver membranes.", "PMID": 978106}
{"id": "pubmed23n0032_11489", "title": "Pre-ovulatory changes in steroidogenesis in ovaries from immature rats treated with pregnant mare serum gonadotrophin.", "content": "The metabolism of progesterone in the 1000 g supernatant fraction of homogenates of ovaries from PMSG-treated immature rats was determined. As early as 52 h after a single injection of 50 i.u. PMSG, still before the LH surge, 5alpha-pregnane-3alpha, 20alpha-diol was identified as the main metabolite, together with small quantities of 3alpha-hydroxy-kalpha-pregnan-20-one and 5alpha-androstane-3alpha, 17beta-diol. Similar incubations of untreated rat ovaries at the same age did not produce 5alpha-pregnane-3alpha, 20alpha-diol. The quantities of 3alpha-hydroxy-5alpha-pregnan-20-one and 5alpha-androstane-3alpha, 1mbeta-diol were reduced in PMSG-treated rat ovaries as compared with control ovaries. When progesterone metabolism was examined 64 h after PMSG administration, 5-7 h after the peak of LH surge but still before ovulation, 75% of the substrate was converted to 5alpha-pregnane-3alpha, 20alpha-diol, while 3alpha-hydroxy-5alpha-pregnan-20-one as well as 5alpha-androstane-3alpha, 17beta-diol could not be detected.", "contents": "Pre-ovulatory changes in steroidogenesis in ovaries from immature rats treated with pregnant mare serum gonadotrophin. The metabolism of progesterone in the 1000 g supernatant fraction of homogenates of ovaries from PMSG-treated immature rats was determined. As early as 52 h after a single injection of 50 i.u. PMSG, still before the LH surge, 5alpha-pregnane-3alpha, 20alpha-diol was identified as the main metabolite, together with small quantities of 3alpha-hydroxy-kalpha-pregnan-20-one and 5alpha-androstane-3alpha, 17beta-diol. Similar incubations of untreated rat ovaries at the same age did not produce 5alpha-pregnane-3alpha, 20alpha-diol. The quantities of 3alpha-hydroxy-5alpha-pregnan-20-one and 5alpha-androstane-3alpha, 1mbeta-diol were reduced in PMSG-treated rat ovaries as compared with control ovaries. When progesterone metabolism was examined 64 h after PMSG administration, 5-7 h after the peak of LH surge but still before ovulation, 75% of the substrate was converted to 5alpha-pregnane-3alpha, 20alpha-diol, while 3alpha-hydroxy-5alpha-pregnan-20-one as well as 5alpha-androstane-3alpha, 17beta-diol could not be detected.", "PMID": 978107}
{"id": "pubmed23n0032_11490", "title": "Biological effects of 131I and 125I isotopes of iodine in the rat.", "content": "From the differences in radiation profiles between 131I and 125I isotopes of iodine it would be expected that they would show different effects on thyroid function. The differences should lead to lower rates of thyroid gland destruction with 125I and hence less post-irradiation hypothyroidism. This difference in biological effect has been demonstrated in rats by indirect assessment of thyroid function. In this report the long-term effects of a range of similar doses of 131I and 125I were compared, in male and female rats, by direct assessment of thyroid function. Seventeen months after receiving 25 and 125 muCi of 131I, male and female rats showed significant elevation of serum TSH concentration and a reduction in 3 h radioiodine uptake. Rats receiving 1 and 5 muCi of 131I and all doses of 125I showed no significant changes in thyroid function. These findings confirm the previously reported differences in effect between the 131I and 125I isotopes of iodine in the rat.", "contents": "Biological effects of 131I and 125I isotopes of iodine in the rat. From the differences in radiation profiles between 131I and 125I isotopes of iodine it would be expected that they would show different effects on thyroid function. The differences should lead to lower rates of thyroid gland destruction with 125I and hence less post-irradiation hypothyroidism. This difference in biological effect has been demonstrated in rats by indirect assessment of thyroid function. In this report the long-term effects of a range of similar doses of 131I and 125I were compared, in male and female rats, by direct assessment of thyroid function. Seventeen months after receiving 25 and 125 muCi of 131I, male and female rats showed significant elevation of serum TSH concentration and a reduction in 3 h radioiodine uptake. Rats receiving 1 and 5 muCi of 131I and all doses of 125I showed no significant changes in thyroid function. These findings confirm the previously reported differences in effect between the 131I and 125I isotopes of iodine in the rat.", "PMID": 978110}
{"id": "pubmed23n0032_11491", "title": "Stimulation of growth in the little mouse.", "content": "The new mouse mutation little (lit) in the homozygous state causes a pituitary deficiency involving at least growth hormone (GH) and prolactin. The resultant growth failure of lit/lit mice was shown to be reversed by experimental conditions that enhanced levels of GH or GH and prolactin in the circulation. Two measures of growth, actual weight gain and bone dimension, were significantly improved by the physiological processes of pregnancy and pseudopregnancy, by extra-sellar graft of a normal mouse pituitary, and by treatment with GH but not prolactin. These data confirmed pituitary dysfunction as the basic defect caused by the mutation lit and showed that the GH deficiency is responsible for growth failure. However, the biological site of gene action, the pituitary or hypothalamus, has not been established. Little mice exhibit a number of characteristics similar to those of human genetic ateleotic dwarfism Type 1, namely genetic inheritance, time of onset of growth retardation, proportionate skeletal size reduction, and pituitary GH deficiency.", "contents": "Stimulation of growth in the little mouse. The new mouse mutation little (lit) in the homozygous state causes a pituitary deficiency involving at least growth hormone (GH) and prolactin. The resultant growth failure of lit/lit mice was shown to be reversed by experimental conditions that enhanced levels of GH or GH and prolactin in the circulation. Two measures of growth, actual weight gain and bone dimension, were significantly improved by the physiological processes of pregnancy and pseudopregnancy, by extra-sellar graft of a normal mouse pituitary, and by treatment with GH but not prolactin. These data confirmed pituitary dysfunction as the basic defect caused by the mutation lit and showed that the GH deficiency is responsible for growth failure. However, the biological site of gene action, the pituitary or hypothalamus, has not been established. Little mice exhibit a number of characteristics similar to those of human genetic ateleotic dwarfism Type 1, namely genetic inheritance, time of onset of growth retardation, proportionate skeletal size reduction, and pituitary GH deficiency.", "PMID": 978118}
{"id": "pubmed23n0032_11492", "title": "Influence of the vagus and carotid sinus nerves on plasma renin in the cat.", "content": "Influence of the vagus and carotid sinus nerves on peripheral plasma renin was studied in the cat. Bilateral cervical vagotomy was followed by a sustained increase in plasma renin concentration (PRC) of anaesthetized cats made hypervolaemic by intravenous saline infusions. The increase in PRC is attributable to right vagotomy. Bilateral carotid artery occlusion in anaesthetized hypervolaemic cats, either with or without prior vagotomy, or with mechanical prevention of a rise in systemic blood pressure, resulted in no statistically significant increases in peripheral venous PRC, compared with the control animals.", "contents": "Influence of the vagus and carotid sinus nerves on plasma renin in the cat. Influence of the vagus and carotid sinus nerves on peripheral plasma renin was studied in the cat. Bilateral cervical vagotomy was followed by a sustained increase in plasma renin concentration (PRC) of anaesthetized cats made hypervolaemic by intravenous saline infusions. The increase in PRC is attributable to right vagotomy. Bilateral carotid artery occlusion in anaesthetized hypervolaemic cats, either with or without prior vagotomy, or with mechanical prevention of a rise in systemic blood pressure, resulted in no statistically significant increases in peripheral venous PRC, compared with the control animals.", "PMID": 978119}
{"id": "pubmed23n0032_11493", "title": "Concentration of oestrogens and androgens in human ovarian venous plasma and follicular fluid throughout the menstrual cycle.", "content": "The concentrations of androstenedione, testosterone, oestrone and oestradiol-17beta were measured in peripheral and ovarian venous blood and follicular fluid of women at various stages of the menstrual cycle. The concentration of oestradiol was similar in small follicles (diameter less than 8 mm) at all stages of the menstrual cycle and in large follicles (diameter greater than or equal to 8 mm) except during the mid- and late follicular phase when the concentration reached a peak (approximately 1500 ng/ml). The concentration of androstenedione was lowest in large preovulatory follicles at midcycle at a time when the secretion into the ovarian vein was markedly increased. The concentration of testosterone in large follicles (greater than or equal to 8 mm) was unchanged during the follicular phase whereas in small follicles there was a peak at mid-cycle. The rise in the concentration of testosterone and androstenedione at mid-cycle in peripheral plasma may be due to increased secretion by the preovulatory follicle into the ovarian vein. It is suggested that the relatively low concentration of androstenedione in follicular fluid of the preovulatory follicle arises from increased aromatization by granulosa cells in the course of oestrogen synthesis.", "contents": "Concentration of oestrogens and androgens in human ovarian venous plasma and follicular fluid throughout the menstrual cycle. The concentrations of androstenedione, testosterone, oestrone and oestradiol-17beta were measured in peripheral and ovarian venous blood and follicular fluid of women at various stages of the menstrual cycle. The concentration of oestradiol was similar in small follicles (diameter less than 8 mm) at all stages of the menstrual cycle and in large follicles (diameter greater than or equal to 8 mm) except during the mid- and late follicular phase when the concentration reached a peak (approximately 1500 ng/ml). The concentration of androstenedione was lowest in large preovulatory follicles at midcycle at a time when the secretion into the ovarian vein was markedly increased. The concentration of testosterone in large follicles (greater than or equal to 8 mm) was unchanged during the follicular phase whereas in small follicles there was a peak at mid-cycle. The rise in the concentration of testosterone and androstenedione at mid-cycle in peripheral plasma may be due to increased secretion by the preovulatory follicle into the ovarian vein. It is suggested that the relatively low concentration of androstenedione in follicular fluid of the preovulatory follicle arises from increased aromatization by granulosa cells in the course of oestrogen synthesis.", "PMID": 978120}
{"id": "pubmed23n0032_11494", "title": "Induction of luteinizing hormone release by gonadal steroids in the ovariectomized domestic hen.", "content": "The ability of intramuscular injections of gonadal steroids to exert a positive feedback action on LH secretion was investigated in the ovariectomized hen. Plasma LH was measured by radioimmunoassay. Single injections of progesterone (dose range: 0.05-10 mg/kg) or oestradiol benzoate (dose range: 0.01-1 mg/kg) did not result in an increase in plasma LH concentration. After priming with 0.1 mg oestradiol benzoate/kg on alternate days for 7 days and with 0.5 mg progesterone/kg on days 5, 6 and 7, a single injection of progesterone on day 8 (dose range: 0.1-2 mg/kg) caused the plasma LH concentration to start increasing after 15 to 30 min. Peak LH concentration was reached around 1.5-2 h after injection. The magnitude of LH response to progesterone was dose related. In contrast, a single injection of oestradiol benzoate (dose range: 0.01-1 mg/kg) failed to stimulate LH release in the oestrogen-progesterone primed ovariectomized (O-P-OVX) hen. A single injection of testosterone (dose range: 0.1-2.0 mg/kg) failed to stimulate LH release in ten out of 12 O-P-OVX hens. A small increase in LH secretion was observed in the two remaining birds. When oestrogen or progesterone was omitted from the priming schedule, a LH positive feedback response to a single injection of progesterone was not observed. Increasing or decreasing the mount of oestrogen or progesterone in the priming schedule modified the LH response to a single injection of progesterone on the day following the last priming injection. This suggested that a critical oestrogen to progesterone ratio was required to prime the LH positive feedback mechanism. It is suggested that, in the hen, the release of LH is facilitated by the positive feedback effect of a combination of oestrogen and progesterone in a two-phase process. The first is the priming phase, which depends on the presence in the blood of oestrogen and progesterone; the second is the ind  .uctive phase, which depends only on an incremental change in plasma progesterone concentration. Oestrogen is not involved in the induceive phase.", "contents": "Induction of luteinizing hormone release by gonadal steroids in the ovariectomized domestic hen. The ability of intramuscular injections of gonadal steroids to exert a positive feedback action on LH secretion was investigated in the ovariectomized hen. Plasma LH was measured by radioimmunoassay. Single injections of progesterone (dose range: 0.05-10 mg/kg) or oestradiol benzoate (dose range: 0.01-1 mg/kg) did not result in an increase in plasma LH concentration. After priming with 0.1 mg oestradiol benzoate/kg on alternate days for 7 days and with 0.5 mg progesterone/kg on days 5, 6 and 7, a single injection of progesterone on day 8 (dose range: 0.1-2 mg/kg) caused the plasma LH concentration to start increasing after 15 to 30 min. Peak LH concentration was reached around 1.5-2 h after injection. The magnitude of LH response to progesterone was dose related. In contrast, a single injection of oestradiol benzoate (dose range: 0.01-1 mg/kg) failed to stimulate LH release in the oestrogen-progesterone primed ovariectomized (O-P-OVX) hen. A single injection of testosterone (dose range: 0.1-2.0 mg/kg) failed to stimulate LH release in ten out of 12 O-P-OVX hens. A small increase in LH secretion was observed in the two remaining birds. When oestrogen or progesterone was omitted from the priming schedule, a LH positive feedback response to a single injection of progesterone was not observed. Increasing or decreasing the mount of oestrogen or progesterone in the priming schedule modified the LH response to a single injection of progesterone on the day following the last priming injection. This suggested that a critical oestrogen to progesterone ratio was required to prime the LH positive feedback mechanism. It is suggested that, in the hen, the release of LH is facilitated by the positive feedback effect of a combination of oestrogen and progesterone in a two-phase process. The first is the priming phase, which depends on the presence in the blood of oestrogen and progesterone; the second is the ind  .uctive phase, which depends only on an incremental change in plasma progesterone concentration. Oestrogen is not involved in the induceive phase.", "PMID": 978121}
{"id": "pubmed23n0032_11495", "title": "Cleft palate induction in hamster fetuses by glucocorticoid hormones and their synthetic analogues.", "content": "The effect of prenatal administration of different doses of cortisone, corticosterone, dexamethasone, triamcinolone and prednisolone on the fetus and its palatal development was studied. All the glucocorticoids, except cortisone, produced cleft palate in the fetuses. Both the total frequency and morphologically different types of cleft palate were related to the dose of the teratogen. Triamcinolone appeared to be more potent than other glucocorticoid in inducing cleft palate. An association was noted between fetal growth inhibition, the dose of the teratogen and the frequency and type of cleft palate.", "contents": "Cleft palate induction in hamster fetuses by glucocorticoid hormones and their synthetic analogues. The effect of prenatal administration of different doses of cortisone, corticosterone, dexamethasone, triamcinolone and prednisolone on the fetus and its palatal development was studied. All the glucocorticoids, except cortisone, produced cleft palate in the fetuses. Both the total frequency and morphologically different types of cleft palate were related to the dose of the teratogen. Triamcinolone appeared to be more potent than other glucocorticoid in inducing cleft palate. An association was noted between fetal growth inhibition, the dose of the teratogen and the frequency and type of cleft palate.", "PMID": 978122}
{"id": "pubmed23n0032_11496", "title": "Distortion of patches of retinal degeneration in chimaeric mice.", "content": "Estimation of the proportion of degenrate retina in rd/rd and +/+ (or +/rd) chimaeras, using a linear analysis of histological sections, suggests a considerable preponderance of the normal phenotype, while analyses based on the area of the surviving tissue suggest that the proportion of each component is similar to that in other tissues. This argues against the validity of the linear analyses used by other authors. It is suggested that cell movement occurs in the outer nuclear layer, following the degeneration of the rd/rd population, and results in a distortion of the original patches of normal cells.", "contents": "Distortion of patches of retinal degeneration in chimaeric mice. Estimation of the proportion of degenrate retina in rd/rd and +/+ (or +/rd) chimaeras, using a linear analysis of histological sections, suggests a considerable preponderance of the normal phenotype, while analyses based on the area of the surviving tissue suggest that the proportion of each component is similar to that in other tissues. This argues against the validity of the linear analyses used by other authors. It is suggested that cell movement occurs in the outer nuclear layer, following the degeneration of the rd/rd population, and results in a distortion of the original patches of normal cells.", "PMID": 978123}
{"id": "pubmed23n0032_11497", "title": "Patches in the livers of chimaeric mice.", "content": "Sections of adult chimaeric livers have been histochemically stained for beta-glucuronidase activity and patches of two cell populations visualized. A one-dimensional clonal analysis has been used to estimate the number of coherent clones in the adult liver. The data are consistent with a total of 9-22 million regular, coherent clones, comprising 10-34 nuclei, or a smaller number of irregular, branched coherent clones. Both of these alternatives suggest considerable cell mixing during liver morphogenesis.", "contents": "Patches in the livers of chimaeric mice. Sections of adult chimaeric livers have been histochemically stained for beta-glucuronidase activity and patches of two cell populations visualized. A one-dimensional clonal analysis has been used to estimate the number of coherent clones in the adult liver. The data are consistent with a total of 9-22 million regular, coherent clones, comprising 10-34 nuclei, or a smaller number of irregular, branched coherent clones. Both of these alternatives suggest considerable cell mixing during liver morphogenesis.", "PMID": 978124}
{"id": "pubmed23n0032_11498", "title": "[Stability of determination and differentiation of the somatic sexual characters in Nereis pelagica L. (Annelida polychaeta)].", "content": "Experiments of regeneration in situ or on grafts were carried out to test the stability of determination of the somatic sexual characters in Nereis pelagica. The sexual characters of the parapodial cirri (male and female swellings, male crenellations) are always expressed on stump or regenerate according to the genetic sex. Supernumary parapodia which developed at the site of junction of the heterologous faces of host and graft of opposite sexes, might have the characters of either sex; this result can be interpreted by supposing that a supernumary parapodium is derived from tissues of either host or graft, of the corresponding sex. Conversely, the pygidial papillae, which normally occur only on the heteronereid male, will appear on regenerating female pygidia, triggered to transform to the heteronereid condition naturally or by decerebration, with a frequency depending on the age of the regenerate, the length of the regenerate, and the genital condition of both the stump and the regenerate. It is concluded that the presence of the papillae is not a sex-specific character, but that their development is normally inhibited in the maturing female.", "contents": "[Stability of determination and differentiation of the somatic sexual characters in Nereis pelagica L. (Annelida polychaeta)]. Experiments of regeneration in situ or on grafts were carried out to test the stability of determination of the somatic sexual characters in Nereis pelagica. The sexual characters of the parapodial cirri (male and female swellings, male crenellations) are always expressed on stump or regenerate according to the genetic sex. Supernumary parapodia which developed at the site of junction of the heterologous faces of host and graft of opposite sexes, might have the characters of either sex; this result can be interpreted by supposing that a supernumary parapodium is derived from tissues of either host or graft, of the corresponding sex. Conversely, the pygidial papillae, which normally occur only on the heteronereid male, will appear on regenerating female pygidia, triggered to transform to the heteronereid condition naturally or by decerebration, with a frequency depending on the age of the regenerate, the length of the regenerate, and the genital condition of both the stump and the regenerate. It is concluded that the presence of the papillae is not a sex-specific character, but that their development is normally inhibited in the maturing female.", "PMID": 978125}
{"id": "pubmed23n0032_11499", "title": "Target discrimination in regenerating insect sensory nerve.", "content": "The paired abdominal cerci of the cricket Acheta domesticus are mechanosensory appendages which regenerate readily when amputated during larval life. Their peripherally-located sense cells form axons which project centrally as a purely sensory nerve to the terminal abdominal ganglion. In an attempt to analyze some of the factors which guide a regenerating sensory nerve to correct central terminations, implants of homologous, supernumerary terminal ganglia were made in cricket larvae and the host cerci amputated. The possibility that implants with multiple nerve stumps might release an attracting substance was considered. Surgical procedures used were (1) implant in posterior abdomen; (2) implant in posterior abdomen, ipsilateral to chronic cercal deprivation; (3) implant in mesothoracic leg socket, adjacent to heterotopically-transplanted regenerated cercus; (4) implant in posterior abdomen, ipsilateral host cercal motor nerve sectioned; (5) implant in posterior abdomen, ipsilateral margin of host terminal ganglion wounded. Results were determined after the adult molt, by conventional histology or by cobalt chloride filling of regenerated cercal nerves. In all procedures except (3) and (4), the regenerated afferent nerve bypassed the implant and terminated in the host terminal ganglion. In (3), the regenerated fibers from cercal grafts bypassed the implant; terminations were not found. In (4), some regenerated cercal axons connected with the implant and the majority terminated in the host ganglion. It is suggested that regenerating cercal afferents may depend in a facultative way on the cercal motor nerve as a pathway guide but there is as yet no clear evidence for a trophic influence from the central nervous system.", "contents": "Target discrimination in regenerating insect sensory nerve. The paired abdominal cerci of the cricket Acheta domesticus are mechanosensory appendages which regenerate readily when amputated during larval life. Their peripherally-located sense cells form axons which project centrally as a purely sensory nerve to the terminal abdominal ganglion. In an attempt to analyze some of the factors which guide a regenerating sensory nerve to correct central terminations, implants of homologous, supernumerary terminal ganglia were made in cricket larvae and the host cerci amputated. The possibility that implants with multiple nerve stumps might release an attracting substance was considered. Surgical procedures used were (1) implant in posterior abdomen; (2) implant in posterior abdomen, ipsilateral to chronic cercal deprivation; (3) implant in mesothoracic leg socket, adjacent to heterotopically-transplanted regenerated cercus; (4) implant in posterior abdomen, ipsilateral host cercal motor nerve sectioned; (5) implant in posterior abdomen, ipsilateral margin of host terminal ganglion wounded. Results were determined after the adult molt, by conventional histology or by cobalt chloride filling of regenerated cercal nerves. In all procedures except (3) and (4), the regenerated afferent nerve bypassed the implant and terminated in the host terminal ganglion. In (3), the regenerated fibers from cercal grafts bypassed the implant; terminations were not found. In (4), some regenerated cercal axons connected with the implant and the majority terminated in the host ganglion. It is suggested that regenerating cercal afferents may depend in a facultative way on the cercal motor nerve as a pathway guide but there is as yet no clear evidence for a trophic influence from the central nervous system.", "PMID": 978126}
{"id": "pubmed23n0032_11500", "title": "Observations on the migration and proliferation of gonocytes in Xenopus laevis.", "content": "The process of primordial germ cell formation in the normal course of development of Xenopus laevis was examined with a light microscope on paraffin and Epon sections of embryos or tadpoles, extending over the period from the gastrula to the feeding tadpole stage. Positional changes of gonocytes with development were nearly the same as those reported on the same species by Blackler (1958) and Whitington & Dixon (1975). The following points were newly demonstrated. Gonocytes which have been located in a deep endodermal position till mid tail-bud stage come to be located in a rather peripheral region of the endoderm cell mass at stage 31 (late tail-bud), suggesting that the initial step of migration of the gonocytes towards the future genital ridge has already begun at this stage. Gonocytes at stages 33/34 and 35/36 were observed in a more dorsal part of the endoderm than at stage 31. Gonocytes which seem to have begun their migration are roundish in external shape and have a large intercellular space around them. At stage 40 gonocytes were located in the dorsal endodermal crest, and at stage 41 gonocytes were found with cell bodies extending over both the dorsal endoderm crest and the dorsal mesentery, indicating that the separation of the gonocytes from the endoderm was in progress at this stage. The present results seem to indicate that gonocytes migrate not passively but actively from the deep endodermal position to the genital ridge, passing through the dorsal mesentery. Counting the number of gonocytes at successive stages of development revealed that gonocytes proliferated exponentially throughout the developmental stages from gastrula to tadpole.", "contents": "Observations on the migration and proliferation of gonocytes in Xenopus laevis. The process of primordial germ cell formation in the normal course of development of Xenopus laevis was examined with a light microscope on paraffin and Epon sections of embryos or tadpoles, extending over the period from the gastrula to the feeding tadpole stage. Positional changes of gonocytes with development were nearly the same as those reported on the same species by Blackler (1958) and Whitington & Dixon (1975). The following points were newly demonstrated. Gonocytes which have been located in a deep endodermal position till mid tail-bud stage come to be located in a rather peripheral region of the endoderm cell mass at stage 31 (late tail-bud), suggesting that the initial step of migration of the gonocytes towards the future genital ridge has already begun at this stage. Gonocytes at stages 33/34 and 35/36 were observed in a more dorsal part of the endoderm than at stage 31. Gonocytes which seem to have begun their migration are roundish in external shape and have a large intercellular space around them. At stage 40 gonocytes were located in the dorsal endodermal crest, and at stage 41 gonocytes were found with cell bodies extending over both the dorsal endoderm crest and the dorsal mesentery, indicating that the separation of the gonocytes from the endoderm was in progress at this stage. The present results seem to indicate that gonocytes migrate not passively but actively from the deep endodermal position to the genital ridge, passing through the dorsal mesentery. Counting the number of gonocytes at successive stages of development revealed that gonocytes proliferated exponentially throughout the developmental stages from gastrula to tadpole.", "PMID": 978127}
{"id": "pubmed23n0032_11501", "title": "Incorporation of tritiated cytochalasin B into ectodermal explants of the amphibian gastrula.", "content": "Ectodermal explants from gastrulae of Triturus alpestris were exposed to tritiated cytochalasin B (5 and 10 mug/ml) for 1/2-2 1/2 h. The flat explants failed to heal into compact spheres and the component cells gradually rounded up. Radioactivity in the fixed and sectioned material was analysed by light and electron microscopic autoradiography. Silver grains were found predominantly over the yolk platelets, but were also scattered over other cellular components. Areas containing pigment granules or the feltwork material, present in the apices of dissociating cells of the superficial layer, exhibited higher activity than areas containing mitochondria or cytoplasmic matrix. Lipid droplets and nuclei showed comparatively little activity. The results are discussed in relation to previous findings on the uptake of tritiated cytochalasin D by cell fractions.", "contents": "Incorporation of tritiated cytochalasin B into ectodermal explants of the amphibian gastrula. Ectodermal explants from gastrulae of Triturus alpestris were exposed to tritiated cytochalasin B (5 and 10 mug/ml) for 1/2-2 1/2 h. The flat explants failed to heal into compact spheres and the component cells gradually rounded up. Radioactivity in the fixed and sectioned material was analysed by light and electron microscopic autoradiography. Silver grains were found predominantly over the yolk platelets, but were also scattered over other cellular components. Areas containing pigment granules or the feltwork material, present in the apices of dissociating cells of the superficial layer, exhibited higher activity than areas containing mitochondria or cytoplasmic matrix. Lipid droplets and nuclei showed comparatively little activity. The results are discussed in relation to previous findings on the uptake of tritiated cytochalasin D by cell fractions.", "PMID": 978128}
{"id": "pubmed23n0032_11502", "title": "[Comparative study of the cells of the freshwater planarian Polycelis tenuis (Iijima) using dissociated fragments cultivated in vitro: ultrastructural aspects and incorporation of (3H) leucine and (3H) uridine].", "content": "Study of planaria cells, which are the result of dissociated fragments cultivated in vitro, allows the evolution of two cell groups to be followed: (1) Differentiated cells, which do not divide, and do not dedifferentiate either, incorporate leucine and uridine at a rate which remains stable for the whole duration of the culture. (2) Undifferentiated cells have a mitotic and incorporation rate of leucine and uridine which varies during the period of culture. After 10 days of culture they demonstrate the characteristics of cells undergoing differentiation. These results are in agreement with those of other authors who attribute an essential role to undifferentiated cells during regeneration of the adult planaria.", "contents": "[Comparative study of the cells of the freshwater planarian Polycelis tenuis (Iijima) using dissociated fragments cultivated in vitro: ultrastructural aspects and incorporation of (3H) leucine and (3H) uridine]. Study of planaria cells, which are the result of dissociated fragments cultivated in vitro, allows the evolution of two cell groups to be followed: (1) Differentiated cells, which do not divide, and do not dedifferentiate either, incorporate leucine and uridine at a rate which remains stable for the whole duration of the culture. (2) Undifferentiated cells have a mitotic and incorporation rate of leucine and uridine which varies during the period of culture. After 10 days of culture they demonstrate the characteristics of cells undergoing differentiation. These results are in agreement with those of other authors who attribute an essential role to undifferentiated cells during regeneration of the adult planaria.", "PMID": 978129}
{"id": "pubmed23n0032_11503", "title": "Cytological and microspectrophotometric analysis of mesodermalized explants of Triturus gastrula ectoderm.", "content": "Using isolated presumptive ectoderm of the newt (Triturus pyrrhogaster) embryos as a reactor and extract of rat bone marrow as a mesodermal inductor, changes of cell number and mitotic index of the reactor cells were studied. In early stages of cultivation the increase in cell number in the mesodermalized ectodermal piece was slower than in the non-mesodermalized epidermal piece; but after 24 h it showed abrupt increase and reached a cell population equal to that of the control at 48 h of cultivation. In the experimental series, the mitotic index was 0 at 4 h after the application of the inducing stimulus, but increased precipitously in the next 8 h and reached a level of 4-4% at 12 h and thereafter decreased gradually. The cell cycle stopped at the S phase and stayed in it for several hours after the application of inductor. A sudden fall in cell number, observed in the mesodermalized epidermal piece between the 4th and the 8th h after the application of inducing stimulus, seems to be attributable to cell death which was brought about by the inducing stimulus. In the histogenetic process phases of repression on mitosis by an inducing stimulus, cell proliferation and nonproliferation seem to succeed each other.", "contents": "Cytological and microspectrophotometric analysis of mesodermalized explants of Triturus gastrula ectoderm. Using isolated presumptive ectoderm of the newt (Triturus pyrrhogaster) embryos as a reactor and extract of rat bone marrow as a mesodermal inductor, changes of cell number and mitotic index of the reactor cells were studied. In early stages of cultivation the increase in cell number in the mesodermalized ectodermal piece was slower than in the non-mesodermalized epidermal piece; but after 24 h it showed abrupt increase and reached a cell population equal to that of the control at 48 h of cultivation. In the experimental series, the mitotic index was 0 at 4 h after the application of the inducing stimulus, but increased precipitously in the next 8 h and reached a level of 4-4% at 12 h and thereafter decreased gradually. The cell cycle stopped at the S phase and stayed in it for several hours after the application of inductor. A sudden fall in cell number, observed in the mesodermalized epidermal piece between the 4th and the 8th h after the application of inducing stimulus, seems to be attributable to cell death which was brought about by the inducing stimulus. In the histogenetic process phases of repression on mitosis by an inducing stimulus, cell proliferation and nonproliferation seem to succeed each other.", "PMID": 978130}
{"id": "pubmed23n0032_11504", "title": "Effect of ascorbic acid deficiency on mouse second molar tooth germs cultivated in vitro.", "content": "Mandibular second molar tooth germs from two-day old mice were cultured in vitro, on millipore membranes, for periods of up to 20 days in liquid medium with or without added ascorbic acid. Tooth germs grown in ascorbate medium were characterized by relatively normal growth, differentiation, morphology and histology. Cuspation patterns were maintained. The epithelial root sheath continued to grow along the millipore membrane. Tooth germs cultured in ascorbate-deficient medium manifested a consistent and striking failure in maintenance of differentiated odontoblastic and ameloblastic tissue with arrest of predentin synthesis, severe structural collapse and reduction in size. Cuspation patterns were lost in scorbutic molars, with sinking of surface layers into pulpal tissue and flattening of the entire organ. This resulted in a lack of recognizable morphology and in severe disorganization of tissues. Only growing areas of the root sheath with associated proliferation of preameloblasts and pre-odontoblasts and adjacent pulpal tissue remained normal and refractory to ascorbate deficiency. Odontoblastic as well as ameloblastic layers were disrupted and cells were dedifferentiated. Newly differentiated odontoblasts became highly vacuolated when they became polarized and started to secrete extracellular matrix.", "contents": "Effect of ascorbic acid deficiency on mouse second molar tooth germs cultivated in vitro. Mandibular second molar tooth germs from two-day old mice were cultured in vitro, on millipore membranes, for periods of up to 20 days in liquid medium with or without added ascorbic acid. Tooth germs grown in ascorbate medium were characterized by relatively normal growth, differentiation, morphology and histology. Cuspation patterns were maintained. The epithelial root sheath continued to grow along the millipore membrane. Tooth germs cultured in ascorbate-deficient medium manifested a consistent and striking failure in maintenance of differentiated odontoblastic and ameloblastic tissue with arrest of predentin synthesis, severe structural collapse and reduction in size. Cuspation patterns were lost in scorbutic molars, with sinking of surface layers into pulpal tissue and flattening of the entire organ. This resulted in a lack of recognizable morphology and in severe disorganization of tissues. Only growing areas of the root sheath with associated proliferation of preameloblasts and pre-odontoblasts and adjacent pulpal tissue remained normal and refractory to ascorbate deficiency. Odontoblastic as well as ameloblastic layers were disrupted and cells were dedifferentiated. Newly differentiated odontoblasts became highly vacuolated when they became polarized and started to secrete extracellular matrix.", "PMID": 978131}
{"id": "pubmed23n0032_11505", "title": "Membrane transport by murine lymphocytes. I. A rapid sampling technique as applied to the adenosine and thymidine systems.", "content": "We have developed a rapid sampling technique for animal cells in suspension for the purpose of measuring membrane transport in lymphocytes. The method involves rapid centrifugation of cells through a layer of silicone oil into perchloric acid after incubation periods as short as 4 s. Using this method we have described the uptake of thymidine and the uptake and transport systems of adenosine by murine bulk nonadherent spleen cells. The two uptake systems are markedly different. Adenosine was shown to be taken up by classical carrier-mediated diffusion, while thymidine was not. In addition we have explored the metabolism of the two nucleosides under the conditions we employed for measuring transport or uptake; Both nucleosides are phosphorylated extensively. We also investigated the uptake and metabolism of thymidine over a 2-h interval the standard time used to measure DNA synthesis in lymphocytes. Unless cells were separated from medium by centrifugation through oil before TCA addition, the TCA precipitable counts exceeded the total radioactive uptake. Hence the standard method for measuring thymidine utilization yields estimates under these conditions which can be as much as 100% too high.", "contents": "Membrane transport by murine lymphocytes. I. A rapid sampling technique as applied to the adenosine and thymidine systems. We have developed a rapid sampling technique for animal cells in suspension for the purpose of measuring membrane transport in lymphocytes. The method involves rapid centrifugation of cells through a layer of silicone oil into perchloric acid after incubation periods as short as 4 s. Using this method we have described the uptake of thymidine and the uptake and transport systems of adenosine by murine bulk nonadherent spleen cells. The two uptake systems are markedly different. Adenosine was shown to be taken up by classical carrier-mediated diffusion, while thymidine was not. In addition we have explored the metabolism of the two nucleosides under the conditions we employed for measuring transport or uptake; Both nucleosides are phosphorylated extensively. We also investigated the uptake and metabolism of thymidine over a 2-h interval the standard time used to measure DNA synthesis in lymphocytes. Unless cells were separated from medium by centrifugation through oil before TCA addition, the TCA precipitable counts exceeded the total radioactive uptake. Hence the standard method for measuring thymidine utilization yields estimates under these conditions which can be as much as 100% too high.", "PMID": 978132}
{"id": "pubmed23n0032_11506", "title": "Induction of IgG in young nude mice by lipid A or thymus grafts.", "content": "Postnatal serum concentrations of IgG2a of paternal allotype, measured in congenitally thymusless nude mice, increase with kinetics and titers comparable to their normal congeneic counterparts. Lipid A, the mitogenic part of LPS, stimulates IgG synthesis in nude mice when it is given 7 days after birth. IgG concentrations at 15 days of age are 6- to 8-fold higher than in untreated control nudes; this is considerably lower, however, than in normal mice, which show up to 45-fold higher IgG2ab levels after lipid A treatment. A thymus graft from nearly congeneic donors of the same age, transplanted at 4 days after birth, also stimulates long-lasting IgG synthesis in the nude recipients. If the grafted nudes are injected with lipid A 3 days later, IgG synthesis is further stimulated 8- to 16-fold. The data are discussed in relation to the thymus dependency of IgG production and the conditions for lipid A stimulation.", "contents": "Induction of IgG in young nude mice by lipid A or thymus grafts. Postnatal serum concentrations of IgG2a of paternal allotype, measured in congenitally thymusless nude mice, increase with kinetics and titers comparable to their normal congeneic counterparts. Lipid A, the mitogenic part of LPS, stimulates IgG synthesis in nude mice when it is given 7 days after birth. IgG concentrations at 15 days of age are 6- to 8-fold higher than in untreated control nudes; this is considerably lower, however, than in normal mice, which show up to 45-fold higher IgG2ab levels after lipid A treatment. A thymus graft from nearly congeneic donors of the same age, transplanted at 4 days after birth, also stimulates long-lasting IgG synthesis in the nude recipients. If the grafted nudes are injected with lipid A 3 days later, IgG synthesis is further stimulated 8- to 16-fold. The data are discussed in relation to the thymus dependency of IgG production and the conditions for lipid A stimulation.", "PMID": 978133}
{"id": "pubmed23n0032_11507", "title": "Alternative pathway of complement: recruitment of precursor properdin by the labile C3/C5 convertase and the potentiation of the pathway.", "content": "In this study the physiological role of properdin and the differential subunit composition of the solid phase enzymes of the pathway have been explored. Cell-bound C3 and C5 convertase differ in their C3b requirement. Apparently one molecule of C3b is sufficient to allow formation of C3 convertase (C3b,B), whereas two or more are required for generation of C5 convertase (C3bn,B). This conclusion was drawn from results indicating the critical role of the spacial distribution of C3b molecules on the cell surface in enzyme formation. While the C3/C5 convertase is fully capable of acting on C5 and thereby initiating the assembly of the cytolytic membrane attack complex, it is exceedingly  labile and vulnerable to destruction by the C3b inactivator. It is the apparent role of properdin to confer a degree of stability upon the labile enzyme and to protect its C3 convertase function against enzymatic destruction. To achieve these effects, precursor properdin (pre-P) is recruited in a binding-activation reaction by the labile C3/C5 convertase. Multiple C3b molecules appear to be needed for the formation of properdin-activating principle. Three modes of regulation have been described, which involve spontaneous dissociation enzymatic degradation by C3b inactivator and disassembly by beta1H. The functional differences of pre-P and activated properdin (P) were delineated, pre-P displaying a weak affinity for C3b and P the capacity of strong interaction, P generating a soluble C3 convertase in serum and pre-P being unable to do so. Because of the profound differences between native pre-P and the laboratory product P, the question was raised as to whether soluble P represents an unphysiological form of the protein. On the basis of this and other studies, the conclusion was reached that in vitro properdin recruitment constitutes the terminal event of the properdin pathway, and that properdin augments the function of C3/C5 convertase without changing its substrate specificity.", "contents": "Alternative pathway of complement: recruitment of precursor properdin by the labile C3/C5 convertase and the potentiation of the pathway. In this study the physiological role of properdin and the differential subunit composition of the solid phase enzymes of the pathway have been explored. Cell-bound C3 and C5 convertase differ in their C3b requirement. Apparently one molecule of C3b is sufficient to allow formation of C3 convertase (C3b,B), whereas two or more are required for generation of C5 convertase (C3bn,B). This conclusion was drawn from results indicating the critical role of the spacial distribution of C3b molecules on the cell surface in enzyme formation. While the C3/C5 convertase is fully capable of acting on C5 and thereby initiating the assembly of the cytolytic membrane attack complex, it is exceedingly  labile and vulnerable to destruction by the C3b inactivator. It is the apparent role of properdin to confer a degree of stability upon the labile enzyme and to protect its C3 convertase function against enzymatic destruction. To achieve these effects, precursor properdin (pre-P) is recruited in a binding-activation reaction by the labile C3/C5 convertase. Multiple C3b molecules appear to be needed for the formation of properdin-activating principle. Three modes of regulation have been described, which involve spontaneous dissociation enzymatic degradation by C3b inactivator and disassembly by beta1H. The functional differences of pre-P and activated properdin (P) were delineated, pre-P displaying a weak affinity for C3b and P the capacity of strong interaction, P generating a soluble C3 convertase in serum and pre-P being unable to do so. Because of the profound differences between native pre-P and the laboratory product P, the question was raised as to whether soluble P represents an unphysiological form of the protein. On the basis of this and other studies, the conclusion was reached that in vitro properdin recruitment constitutes the terminal event of the properdin pathway, and that properdin augments the function of C3/C5 convertase without changing its substrate specificity.", "PMID": 978134}
{"id": "pubmed23n0032_11508", "title": "Inherited structural polymorphism in human C2: evidence for genetic linkage between C2 and Bf.", "content": "Structural variation in the second component of human complement was identified in about 4% of serum samples from random unrelated individuals of all the major races. Three forms of C2 have been identified by isoelectric focusing in polyacrylamide gel and development of patterns in agarose gel containing antibody-sensitized sheep red cells and C2-deficient serum: C2 C (common), C2 A (acidic), and C2 B (basic). The C2 variants were shown to be inherited as autosomal codominant traits, and suggestive evidence for close linkage between C2 and Bf was obtained.", "contents": "Inherited structural polymorphism in human C2: evidence for genetic linkage between C2 and Bf. Structural variation in the second component of human complement was identified in about 4% of serum samples from random unrelated individuals of all the major races. Three forms of C2 have been identified by isoelectric focusing in polyacrylamide gel and development of patterns in agarose gel containing antibody-sensitized sheep red cells and C2-deficient serum: C2 C (common), C2 A (acidic), and C2 B (basic). The C2 variants were shown to be inherited as autosomal codominant traits, and suggestive evidence for close linkage between C2 and Bf was obtained.", "PMID": 978135}
{"id": "pubmed23n0032_11509", "title": "Murine model for human secondary amyloidosis: genetic variability of the acute-phase serum protein SAA response to endotoxins and casein.", "content": "The serum precursor SAA of the secondary amyloid protein AA has been detected by solid-phase radioimmunoassay as a normal serum alpha-globulin of mol wt 160,000, which dissociates to a more stable 12,500 dalton moiety on treatment with formic acid. In 12 strains of mice, including T-cell-deficient nude mice, treated with the amyloid-inducing agents lipopolysaccharide (LPS) or casein, SAA behaved as an acute-phase reactant. SAA concentration rose to about 750 mug/ml by 24 h and returned to less than 1 mug/ml by 48 h. Since the amyloid-resistant colchicine-treated mice and AJ mice had a normal SAA response to LPS, it appears that their resistance to amyloid induction is due to the nature of their SAA processing rather than decreased SAA production. C3H/HeJ mice, which have defective B-lymphocyte responses to LPS, required extremely high dosages of LPS to cause SAA elevation, although their SAA response to casein was normal. This suggests that SAA is an acute-phase protein produced as a result of B-lymphocyte stimulation. Preliminary evidence suggests that at the height of an acute SAA response, liver homogenates are particularly rich in protein AA cross-reacting material.", "contents": "Murine model for human secondary amyloidosis: genetic variability of the acute-phase serum protein SAA response to endotoxins and casein. The serum precursor SAA of the secondary amyloid protein AA has been detected by solid-phase radioimmunoassay as a normal serum alpha-globulin of mol wt 160,000, which dissociates to a more stable 12,500 dalton moiety on treatment with formic acid. In 12 strains of mice, including T-cell-deficient nude mice, treated with the amyloid-inducing agents lipopolysaccharide (LPS) or casein, SAA behaved as an acute-phase reactant. SAA concentration rose to about 750 mug/ml by 24 h and returned to less than 1 mug/ml by 48 h. Since the amyloid-resistant colchicine-treated mice and AJ mice had a normal SAA response to LPS, it appears that their resistance to amyloid induction is due to the nature of their SAA processing rather than decreased SAA production. C3H/HeJ mice, which have defective B-lymphocyte responses to LPS, required extremely high dosages of LPS to cause SAA elevation, although their SAA response to casein was normal. This suggests that SAA is an acute-phase protein produced as a result of B-lymphocyte stimulation. Preliminary evidence suggests that at the height of an acute SAA response, liver homogenates are particularly rich in protein AA cross-reacting material.", "PMID": 978136}
{"id": "pubmed23n0032_11510", "title": "In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules.", "content": "Two neutral proteinases from human polymorphonuclear leukocytes (PMN), an elastase and the chymotrypsin-like cathepsin G, were purified, and their actions on lymphocytes in culture were studied. Both PMN proteinases stimulate lymphocytes from human peripheral blood and from mouse spleen in vitro, but do not affect thymic cells from either normal or hydrocortisone-treated mice. In stimulated mouse spleen cell cultures, most of the developing blast cells bear surface immunoglobulins, and subsequently appear to engage in antibody synthesis. In their stimulatory action, the two PMN proteinases thus resemble the classic B-cell mitogen LPS and neutral pancreatic proteinases such as trypsin, chymotrypsin, and elastase. The effects of proteinase inhibitors indicate that lymphocyte stimulation is dependent on the proteolytic activity of the enzymes. This work suggests that PMN proteinases, which are released at sites of inflammation, may modulate the function of lymphocytes.", "contents": "In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules. Two neutral proteinases from human polymorphonuclear leukocytes (PMN), an elastase and the chymotrypsin-like cathepsin G, were purified, and their actions on lymphocytes in culture were studied. Both PMN proteinases stimulate lymphocytes from human peripheral blood and from mouse spleen in vitro, but do not affect thymic cells from either normal or hydrocortisone-treated mice. In stimulated mouse spleen cell cultures, most of the developing blast cells bear surface immunoglobulins, and subsequently appear to engage in antibody synthesis. In their stimulatory action, the two PMN proteinases thus resemble the classic B-cell mitogen LPS and neutral pancreatic proteinases such as trypsin, chymotrypsin, and elastase. The effects of proteinase inhibitors indicate that lymphocyte stimulation is dependent on the proteolytic activity of the enzymes. This work suggests that PMN proteinases, which are released at sites of inflammation, may modulate the function of lymphocytes.", "PMID": 978137}
{"id": "pubmed23n0032_11511", "title": "Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.", "content": "Immune adherence assays revealed that 10 out of 18 melanoma patients had demonstrable antibody to surface antigens of autologous cultured melanoma cells, with serum titers ranging from 1/4 to 1/160. Autologous fibroblasts showed no reactions with these sera. Antibody from individual patients showed reproducible temperature preference for maximal reactivity. Two new melanoma antigenic systems were defined in this study. The first, BD, was restricted to autologous melanoma and could not be demonstrated in absorption tests on 12 allogeneic melanoma cell lines. The other, AH, was found on 5 of 12 melanomas and represents a class of shared melanoma surface antigens. Neither BD nor AH antigen was found on normal cells from autologous, allogeneic, or xenogeneic sources or on any nonmelanoma tumor cell line. Methods are now available to develop a comprehensive serological classification of the surface antigens of melanoma.", "contents": "Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens. Immune adherence assays revealed that 10 out of 18 melanoma patients had demonstrable antibody to surface antigens of autologous cultured melanoma cells, with serum titers ranging from 1/4 to 1/160. Autologous fibroblasts showed no reactions with these sera. Antibody from individual patients showed reproducible temperature preference for maximal reactivity. Two new melanoma antigenic systems were defined in this study. The first, BD, was restricted to autologous melanoma and could not be demonstrated in absorption tests on 12 allogeneic melanoma cell lines. The other, AH, was found on 5 of 12 melanomas and represents a class of shared melanoma surface antigens. Neither BD nor AH antigen was found on normal cells from autologous, allogeneic, or xenogeneic sources or on any nonmelanoma tumor cell line. Methods are now available to develop a comprehensive serological classification of the surface antigens of melanoma.", "PMID": 978138}
{"id": "pubmed23n0032_11512", "title": "Natural concepts in pigeons.", "content": "Pigeons learned to discriminate pictures of trees, bodies of water, or a particular person in three separate experiments. Pictures being seen for the first time were discriminated almost as well as pictures seen in training. The pigeons in each experiment showed similar patterns of errors and correct discrimination.", "contents": "Natural concepts in pigeons. Pigeons learned to discriminate pictures of trees, bodies of water, or a particular person in three separate experiments. Pictures being seen for the first time were discriminated almost as well as pictures seen in training. The pigeons in each experiment showed similar patterns of errors and correct discrimination.", "PMID": 978139}
{"id": "pubmed23n0032_11513", "title": "Aversions to unflavored water and cup drinking produced by delayed sickness.", "content": "Rats drank from a cup and were then injected with lithium chloride. The result was an apparent association between the appearance of the cup and toxicosis. In Experiment 1, avoidance of drinking unflavored water from the cup was apparent after two trials in which toxicosis was induced either immediately or 1/2 hr. after such drinking. Consumption of sucrose solution from the cup prior to toxicosis, however, did not produce a statistically reliable aversion to drinking unflavored water from the cup. In Experiment 2, rats were repeatedly subjected to toxicosis 0.0, .5, 1.5, 4.5, or 18.0 hr. after drinking unflavored water from a cup. A reduction in drinking from the cup was inversely related to the delay of toxicosis, but only the 0.0- and .5-hr.-delay groups exhibited statistically reliable aversions. A later phase excluded the possibility that sensitization alone could account for the cup aversions. Marked aversions to the unflavored water itself were obtained in Experiment 1, but in Experiment 2 any such aversions were so weak that they could not have been responsible for the cup aversions.", "contents": "Aversions to unflavored water and cup drinking produced by delayed sickness. Rats drank from a cup and were then injected with lithium chloride. The result was an apparent association between the appearance of the cup and toxicosis. In Experiment 1, avoidance of drinking unflavored water from the cup was apparent after two trials in which toxicosis was induced either immediately or 1/2 hr. after such drinking. Consumption of sucrose solution from the cup prior to toxicosis, however, did not produce a statistically reliable aversion to drinking unflavored water from the cup. In Experiment 2, rats were repeatedly subjected to toxicosis 0.0, .5, 1.5, 4.5, or 18.0 hr. after drinking unflavored water from a cup. A reduction in drinking from the cup was inversely related to the delay of toxicosis, but only the 0.0- and .5-hr.-delay groups exhibited statistically reliable aversions. A later phase excluded the possibility that sensitization alone could account for the cup aversions. Marked aversions to the unflavored water itself were obtained in Experiment 1, but in Experiment 2 any such aversions were so weak that they could not have been responsible for the cup aversions.", "PMID": 978140}
{"id": "pubmed23n0032_11514", "title": "Effects of priming unconditioned stimulus representation in short-term memory on Pavlovian conditioning.", "content": "A series of four experiments evaluated eyelid conditioning in the rabbit under circumstances in which the unconditioned stimulus (US) could variously be assumed to be prerepresented (primed) in short-term memory at the time of its presentation in pairing with a conditioned stimulus (CS). In both between- and within-subjects comparisons, less conditioning resulted when CS-US pairings were shortly preceded by US presentations than when not. The decremental effect of the pretrial priming US depended upon the similarity of such US to the subsequent conditioning US included in the CS-US pairing and could be removed by the interpolation of a distractor stimulus between the priming US and the CS-US pair. Finally, a similar decrement in conditioning was observed if a weak priming US was administered prior to the conditioning US but after the CS, that is, within the CS-US interval. The overall pattern of data supports Wagner's thesis that stimuli are rendered generally less effective when they are primed in short-term memory.", "contents": "Effects of priming unconditioned stimulus representation in short-term memory on Pavlovian conditioning. A series of four experiments evaluated eyelid conditioning in the rabbit under circumstances in which the unconditioned stimulus (US) could variously be assumed to be prerepresented (primed) in short-term memory at the time of its presentation in pairing with a conditioned stimulus (CS). In both between- and within-subjects comparisons, less conditioning resulted when CS-US pairings were shortly preceded by US presentations than when not. The decremental effect of the pretrial priming US depended upon the similarity of such US to the subsequent conditioning US included in the CS-US pairing and could be removed by the interpolation of a distractor stimulus between the priming US and the CS-US pair. Finally, a similar decrement in conditioning was observed if a weak priming US was administered prior to the conditioning US but after the CS, that is, within the CS-US interval. The overall pattern of data supports Wagner's thesis that stimuli are rendered generally less effective when they are primed in short-term memory.", "PMID": 978141}
{"id": "pubmed23n0032_11515", "title": "Limulus psychophysics: spectral sensitivity of the ventral eye.", "content": "The ventral eye of Limulus contains only one type of photoreceptor. Behaviors mediated by the ventral eye provide an unambiguous representation of the function of that single-receptor type. Such behaviors can be compared with the results of acute, single-cell investigations to assay for the contributions of candidate neural codes in the regulation of behavior (cf. Uttal, 1973). Using an unconditioned tail movement as the response, the psychophysical spectral sensitivity function mediated by the ventral eye of Limulus was measured. This psychophysical function peaked at 525 nm and showed evidence of strong absorption by the cuticle in the short-wavelength portion of the spectrum. Under the conditions of the present experiment, the threshold was 4.5 quanta absorbed per receptor per msec at 525 nm. The spectral transmission of the ventral eye cuticle was also measured. After correction for cuticle absorption, the psychophysical spectral sensitivity function was compared with previously reported spectral sensitivity functions obtained either from electrophysiologic (Millecchia, Bradbury, and Mauro, 1966; Nolte and Brown, 1970) or from microspectrophotometric (Murry, 1966) recordings from single, isolated ventral eye photoreceptor cells. All three functions exhibit a sensitivity peak near 525 nm; the corrected psychophysical and microspectrophotometric functions both display a second peak near 425 nm. A second experiment confirmed the reliability and validity of the 425-nm peak. The coding implications of these findings were explored. A preliminary finding is that, in dichromatic or trichromatic visual systems, two-peaked receptor spectral sensitivity functions produce central, opponent response systems that are qualitatively the same as those produced by single-peaked receptors.", "contents": "Limulus psychophysics: spectral sensitivity of the ventral eye. The ventral eye of Limulus contains only one type of photoreceptor. Behaviors mediated by the ventral eye provide an unambiguous representation of the function of that single-receptor type. Such behaviors can be compared with the results of acute, single-cell investigations to assay for the contributions of candidate neural codes in the regulation of behavior (cf. Uttal, 1973). Using an unconditioned tail movement as the response, the psychophysical spectral sensitivity function mediated by the ventral eye of Limulus was measured. This psychophysical function peaked at 525 nm and showed evidence of strong absorption by the cuticle in the short-wavelength portion of the spectrum. Under the conditions of the present experiment, the threshold was 4.5 quanta absorbed per receptor per msec at 525 nm. The spectral transmission of the ventral eye cuticle was also measured. After correction for cuticle absorption, the psychophysical spectral sensitivity function was compared with previously reported spectral sensitivity functions obtained either from electrophysiologic (Millecchia, Bradbury, and Mauro, 1966; Nolte and Brown, 1970) or from microspectrophotometric (Murry, 1966) recordings from single, isolated ventral eye photoreceptor cells. All three functions exhibit a sensitivity peak near 525 nm; the corrected psychophysical and microspectrophotometric functions both display a second peak near 425 nm. A second experiment confirmed the reliability and validity of the 425-nm peak. The coding implications of these findings were explored. A preliminary finding is that, in dichromatic or trichromatic visual systems, two-peaked receptor spectral sensitivity functions produce central, opponent response systems that are qualitatively the same as those produced by single-peaked receptors.", "PMID": 978142}
{"id": "pubmed23n0032_11516", "title": "Reform in the United States: its impact on medicine and education for family practice.", "content": "The historical concept of reform is useful as an aid to understand the modern rise of family practice education. Beginning about 1890, historians have identified several themes of reform in the United States which have been expressed culturally, politically, and socially. Each of these themes, agrarianism, bureaucratization of the professions, and utopianism, has influenced medicine and medical education--first at the turn of the century in the activities of the AMA in promoting public health and in establishing the natural sciences as a basis for medical education and practice. Since the end of World War II, additional reform themes have become visible which are also influencing medicine. Among these are humanism, consumerism, and the women's movement. It is the author's thesis that the present vitality and future development of family practice as a discipline is more dependent on its capacity and willingness to be identified with these expressions of reform than on its negotiations and compromises within the medical education establishment.", "contents": "Reform in the United States: its impact on medicine and education for family practice. The historical concept of reform is useful as an aid to understand the modern rise of family practice education. Beginning about 1890, historians have identified several themes of reform in the United States which have been expressed culturally, politically, and socially. Each of these themes, agrarianism, bureaucratization of the professions, and utopianism, has influenced medicine and medical education--first at the turn of the century in the activities of the AMA in promoting public health and in establishing the natural sciences as a basis for medical education and practice. Since the end of World War II, additional reform themes have become visible which are also influencing medicine. Among these are humanism, consumerism, and the women's movement. It is the author's thesis that the present vitality and future development of family practice as a discipline is more dependent on its capacity and willingness to be identified with these expressions of reform than on its negotiations and compromises within the medical education establishment.", "PMID": 978147}
{"id": "pubmed23n0032_11517", "title": "An information system for family practice. Part 1: Defining the practice population.", "content": "Information systems for family practice are vital in its development as an academic discipline, in the teaching and learning process which ultimately improves the quality of care, and in the planning processes which must be used to rationalize the distribution of scarce resources in the health-care field. The provision of sufficient data for these purposes demands a flexible system, generally one based on the computer as a data storing and analyzing tool. Such a system has been developed in the teaching practices of the Department of Family Medicine, University of Western Ontario; the methods employed and the uses for that system are to be described in a series of four papers. This first paper describes the methods developed for gathering demographic information on the practice population.", "contents": "An information system for family practice. Part 1: Defining the practice population. Information systems for family practice are vital in its development as an academic discipline, in the teaching and learning process which ultimately improves the quality of care, and in the planning processes which must be used to rationalize the distribution of scarce resources in the health-care field. The provision of sufficient data for these purposes demands a flexible system, generally one based on the computer as a data storing and analyzing tool. Such a system has been developed in the teaching practices of the Department of Family Medicine, University of Western Ontario; the methods employed and the uses for that system are to be described in a series of four papers. This first paper describes the methods developed for gathering demographic information on the practice population.", "PMID": 978148}
{"id": "pubmed23n0032_11518", "title": "An information system for family practice. Part 2: The value of defining a practice population.", "content": "The gathering of information on the practice population is essential for practice monitoring, preventive medicine, and research. The minimum necessary information is the age and sex of individuals cared for in the practice. This allows the expression of age-sex specific morbidity rates for the at-risk population. In the Department of Family Medicine, University of Western Ontario, practice census information has been used to determine the suitable size for a teaching practice, the representativeness of our practices in terms of age, sex, residence location, and morbidity, and for the production of at-risk registers based on age and sex.", "contents": "An information system for family practice. Part 2: The value of defining a practice population. The gathering of information on the practice population is essential for practice monitoring, preventive medicine, and research. The minimum necessary information is the age and sex of individuals cared for in the practice. This allows the expression of age-sex specific morbidity rates for the at-risk population. In the Department of Family Medicine, University of Western Ontario, practice census information has been used to determine the suitable size for a teaching practice, the representativeness of our practices in terms of age, sex, residence location, and morbidity, and for the production of at-risk registers based on age and sex.", "PMID": 978149}
{"id": "pubmed23n0032_11519", "title": "Injection therapy for varicose veins.", "content": "The varicose tendency cannot be cured but it can be controlled. Injection compression sclerotherapy, if performed competently on selected patients, is an effective treatment. It is a relatively simple, economical, safe, and ambulatory form of therapy which has a major place in the management of varicose veins. The technique for injection is subject to considerable variation, but the necessity for post injection compression of the veins is absolute. Post injection compression reduces the potential for recurrence by preventing return of the blood to the vein after injection and maintaining contact of the intimal surfaces so that bonding can occur, thus converting the vein from a tube to a solid fibrotic cord. Injection compression sclerotherapy is the best treatment of selected varicosities.", "contents": "Injection therapy for varicose veins. The varicose tendency cannot be cured but it can be controlled. Injection compression sclerotherapy, if performed competently on selected patients, is an effective treatment. It is a relatively simple, economical, safe, and ambulatory form of therapy which has a major place in the management of varicose veins. The technique for injection is subject to considerable variation, but the necessity for post injection compression of the veins is absolute. Post injection compression reduces the potential for recurrence by preventing return of the blood to the vein after injection and maintaining contact of the intimal surfaces so that bonding can occur, thus converting the vein from a tube to a solid fibrotic cord. Injection compression sclerotherapy is the best treatment of selected varicosities.", "PMID": 978150}
{"id": "pubmed23n0032_11520", "title": "On the diversity of sperm histones in the vertebrates: II. A cytochemical study of the basic protein transitions during spermiogenesis in the cartilaginous fish Hydrolagus colliei.", "content": "Spermiogenesis in the ratfish (Hydrolagus colliei) is characterized by unusual changes in the basic proteins of the nucleus. Cytochemical analysis indicates that early spermatids in this cartilaginous fish contain the somatic type of histones. However, late spermatids contain the salmon type of sperm histone whereas spermatozoa display the mouse/grasshopper type designated by Bloch ('69). Such an unusual cytochemical shift from protamine in the spermatid to \"stable protamine\" in the sperm has not been documented heretofore.", "contents": "On the diversity of sperm histones in the vertebrates: II. A cytochemical study of the basic protein transitions during spermiogenesis in the cartilaginous fish Hydrolagus colliei. Spermiogenesis in the ratfish (Hydrolagus colliei) is characterized by unusual changes in the basic proteins of the nucleus. Cytochemical analysis indicates that early spermatids in this cartilaginous fish contain the somatic type of histones. However, late spermatids contain the salmon type of sperm histone whereas spermatozoa display the mouse/grasshopper type designated by Bloch ('69). Such an unusual cytochemical shift from protamine in the spermatid to \"stable protamine\" in the sperm has not been documented heretofore.", "PMID": 978157}
{"id": "pubmed23n0032_11521", "title": "Body temperature of the lizard (Anolis carolinensis): effect of parietalectomy.", "content": "Thermal behavior of parietalectomized, sham-parietalectomized or intact lizards (Anolis carolinensis) was recorded in a test chamber containing moving photothermal gradients. Following parietalectomy, animals chose significantly higher temperatures than sham-parietalectomized or intact control animals, which chose lower and statistically identical temperatures. When pre-surgical and post-surgical means are compared within groups, the eccritic temperature was 2.10 degrees C higher following parietalectomy, 0.72 degrees C higher following sham-parietalectomy, and 0.31 degrees C higher in intact controls during the post-operative measurements. The effects of parietalectomy indicate that the parietalpineal complex may be involved in behavioral thermoregulation by precisely regulating exposure to heat sources (e.g. sunlight).", "contents": "Body temperature of the lizard (Anolis carolinensis): effect of parietalectomy. Thermal behavior of parietalectomized, sham-parietalectomized or intact lizards (Anolis carolinensis) was recorded in a test chamber containing moving photothermal gradients. Following parietalectomy, animals chose significantly higher temperatures than sham-parietalectomized or intact control animals, which chose lower and statistically identical temperatures. When pre-surgical and post-surgical means are compared within groups, the eccritic temperature was 2.10 degrees C higher following parietalectomy, 0.72 degrees C higher following sham-parietalectomy, and 0.31 degrees C higher in intact controls during the post-operative measurements. The effects of parietalectomy indicate that the parietalpineal complex may be involved in behavioral thermoregulation by precisely regulating exposure to heat sources (e.g. sunlight).", "PMID": 978158}
{"id": "pubmed23n0032_11522", "title": "Parietalectomy and thermal selection in the lizard Sceloporus magister.", "content": "Lizards were acclimatized to various regimes of temperature (15 degrees, 35 degrees C) and photoperiod (LD 08:16, 16:8). Parietalectomized, sham operated, and control animals were placed in thermal gradients and their body temperatures monitored for periods of up to 15 days. Daily rhythms of thermal selection were evident with higher temperatures selected in late photophase and lower temperatures selected in scotophase. Thermal selection was more variable in scotophase than in photophase. Parietalectomized lizards chose significantly higher body temperatures than did shams or controls. Acclimatization to temperature and photoperiod had little or no effect on the thermal preferendum. The preferred body temperature of controls was 33.2 degrees C +/- 3.59. Length of time in the gradient tended to change thermal preference, especially in lizards acclimated to 15 degrees C. Through interactions with the pineal gland the parietal eye is probably important in synchronizing many bodily functions with photoperiod.", "contents": "Parietalectomy and thermal selection in the lizard Sceloporus magister. Lizards were acclimatized to various regimes of temperature (15 degrees, 35 degrees C) and photoperiod (LD 08:16, 16:8). Parietalectomized, sham operated, and control animals were placed in thermal gradients and their body temperatures monitored for periods of up to 15 days. Daily rhythms of thermal selection were evident with higher temperatures selected in late photophase and lower temperatures selected in scotophase. Thermal selection was more variable in scotophase than in photophase. Parietalectomized lizards chose significantly higher body temperatures than did shams or controls. Acclimatization to temperature and photoperiod had little or no effect on the thermal preferendum. The preferred body temperature of controls was 33.2 degrees C +/- 3.59. Length of time in the gradient tended to change thermal preference, especially in lizards acclimated to 15 degrees C. Through interactions with the pineal gland the parietal eye is probably important in synchronizing many bodily functions with photoperiod.", "PMID": 978159}
{"id": "pubmed23n0032_11523", "title": "Phospholipase activity of sea urchin sperm: its possible involvement in membrane fusion.", "content": "Phospholipase activity of egg-water treated Arbacia punctulata and Lytechinus variegatus sperm was shown to result from the sequential action of phospholipase A and lysophospholipase. A transient burst of phospholipase A activity followed induction of the acrosome reaction with egg water. The time of appearance suggested an acrosomal localization of the enzyme. The peak activity of phospholipase A correlated with initiation of sperm-egg fusion, suggesting a role for sea urchin sperm phospholipase A in membrane fusion and/or egg activation during fertilization.", "contents": "Phospholipase activity of sea urchin sperm: its possible involvement in membrane fusion. Phospholipase activity of egg-water treated Arbacia punctulata and Lytechinus variegatus sperm was shown to result from the sequential action of phospholipase A and lysophospholipase. A transient burst of phospholipase A activity followed induction of the acrosome reaction with egg water. The time of appearance suggested an acrosomal localization of the enzyme. The peak activity of phospholipase A correlated with initiation of sperm-egg fusion, suggesting a role for sea urchin sperm phospholipase A in membrane fusion and/or egg activation during fertilization.", "PMID": 978160}
{"id": "pubmed23n0032_11524", "title": "28 s and 18 s ribonucleic acid from mammalian spermatozoa.", "content": "The majority of RNA is spermatozoa from the epididymis and ductus deferens of mice was found to be 28 s and 18 s RNA. This RNA was also seen in human ejaculated spermatozoa. Our evidence indicates that little or none of the 28 s and 18 s RNA is transcribed in the maturing mouse spermatozoa and that the majority is synthesized in the primary spermatocyte. This RNA may be necessary to maintain post-meiotic translation during spermatogenesis.", "contents": "28 s and 18 s ribonucleic acid from mammalian spermatozoa. The majority of RNA is spermatozoa from the epididymis and ductus deferens of mice was found to be 28 s and 18 s RNA. This RNA was also seen in human ejaculated spermatozoa. Our evidence indicates that little or none of the 28 s and 18 s RNA is transcribed in the maturing mouse spermatozoa and that the majority is synthesized in the primary spermatocyte. This RNA may be necessary to maintain post-meiotic translation during spermatogenesis.", "PMID": 978161}
{"id": "pubmed23n0032_11525", "title": "Muscle satellite cells in urodele amphibians: faciliatated identification of satellite cells using ruthenium red staining.", "content": "The ruthenium red (RR) stained forelimb musculature of three species of urodeles Triturus (Notophthalmus) viridescens, Amblystoma maculatum, Amblystoma opacum in various stages of growth were examined with the electron microscope for the presence of satellite cells. It was found that RR staining facilitated greatly the identification of satellite cells. In young larvae of all three species satellite cells were detected with a frequency of 29% to 48% per total number of nuclei. In adult Triturus and Amblystoma maculatum satellite cells were no longer detected; instead \"pericytes\" as described by Hay ('74) were seen with a frequency of 12% and 3% respectively. During metamorphosis of Triturus satellite cells, with part of their myofiber-satellite cell intercellular space filled with basement membrane material, occurred at a peak frequency. The cells presumably are intermediate in the formation of \"pericytes.\" At ten days after metamorphosis satellite cells and intermediate cells were no longer detected and the limb musculature contained only \"pericytes\" similar to the ones observed in adult newts. The significance of the presence of satellite cells in relation to limb regeneration and muscle regeneration is discussed.", "contents": "Muscle satellite cells in urodele amphibians: faciliatated identification of satellite cells using ruthenium red staining. The ruthenium red (RR) stained forelimb musculature of three species of urodeles Triturus (Notophthalmus) viridescens, Amblystoma maculatum, Amblystoma opacum in various stages of growth were examined with the electron microscope for the presence of satellite cells. It was found that RR staining facilitated greatly the identification of satellite cells. In young larvae of all three species satellite cells were detected with a frequency of 29% to 48% per total number of nuclei. In adult Triturus and Amblystoma maculatum satellite cells were no longer detected; instead \"pericytes\" as described by Hay ('74) were seen with a frequency of 12% and 3% respectively. During metamorphosis of Triturus satellite cells, with part of their myofiber-satellite cell intercellular space filled with basement membrane material, occurred at a peak frequency. The cells presumably are intermediate in the formation of \"pericytes.\" At ten days after metamorphosis satellite cells and intermediate cells were no longer detected and the limb musculature contained only \"pericytes\" similar to the ones observed in adult newts. The significance of the presence of satellite cells in relation to limb regeneration and muscle regeneration is discussed.", "PMID": 978162}
{"id": "pubmed23n0032_11526", "title": "The role of divalent cations in activation of the sea urchin egg. I. Effect of fertilization on divalent cation content.", "content": "The Ca and Mg content of unfertilized sea urchin eggs (3 and 21 mumole/ml eggs) remains remarkably constant over periods of hours, even when the eggs are suspended in Ca- or Mg-free sea water. After fertilization the Ca content of eggs in regular sea water increases sharply by about 20%, followed by a decrease to the unfertilized level by 40 minutes. However, if the fertilized eggs are washed three minutes in Ca-free sea water a sharp decrease in the Ca content occurs amounting to 30% of the total in the first 40 minutes, with little change thereafter. Suspension of the eggs in Ca-free sea water results in an even greater loss of Ca amounting to about 43% of the total in the same time interval, followed by a continuing slow loss. It is concluded that fertilization initiates the intracellular release of Ca, which is then extruded. For unwashed eggs this change is masked by the simultaneous generation of new extra-cellular coats with high affinity for Ca. Changes in the Mg content of fertilized eggs follow the same general pattern except that absorption of this divalent cation to the extracellular coats is minimal.", "contents": "The role of divalent cations in activation of the sea urchin egg. I. Effect of fertilization on divalent cation content. The Ca and Mg content of unfertilized sea urchin eggs (3 and 21 mumole/ml eggs) remains remarkably constant over periods of hours, even when the eggs are suspended in Ca- or Mg-free sea water. After fertilization the Ca content of eggs in regular sea water increases sharply by about 20%, followed by a decrease to the unfertilized level by 40 minutes. However, if the fertilized eggs are washed three minutes in Ca-free sea water a sharp decrease in the Ca content occurs amounting to 30% of the total in the first 40 minutes, with little change thereafter. Suspension of the eggs in Ca-free sea water results in an even greater loss of Ca amounting to about 43% of the total in the same time interval, followed by a continuing slow loss. It is concluded that fertilization initiates the intracellular release of Ca, which is then extruded. For unwashed eggs this change is masked by the simultaneous generation of new extra-cellular coats with high affinity for Ca. Changes in the Mg content of fertilized eggs follow the same general pattern except that absorption of this divalent cation to the extracellular coats is minimal.", "PMID": 978163}
{"id": "pubmed23n0032_11527", "title": "Ultrastructural characterization of the accessory lobes of Lachi (Hofmann's nuclei) in the nerve cord of the chick. I. Axoglial synapses.", "content": "Light microscopic observations of the accessory lobes of Lachi of one-day-old chicks show that this tissue contains abundant amounts of glycogen and consists of cells which are similar in appearance to those of the glycogen body. Ultrastructural studies reported here for the first time confirm the presence of glycogen-rich cells in the accessory lobes and reveal that these cells are intimately associated with nerve axons. The finding of synaptic complexes and other junctional specializations between nerves and accessory lobe cells suggests that they may have a functional relationship with the nervous system. It is felt that the accessory lobe cells may be neuroglia, possibly of the astrocytic type, which have an innate capacity for glycogen storage. While the functional significance of such glycogen remains obscure, the close morphological association between neurons and the accessory lobe cells enhances the hypothesis put forth by us regarding the glycogen body, namely that neural glycogen is involved in myelin synthesis in the avian nervous system.", "contents": "Ultrastructural characterization of the accessory lobes of Lachi (Hofmann's nuclei) in the nerve cord of the chick. I. Axoglial synapses. Light microscopic observations of the accessory lobes of Lachi of one-day-old chicks show that this tissue contains abundant amounts of glycogen and consists of cells which are similar in appearance to those of the glycogen body. Ultrastructural studies reported here for the first time confirm the presence of glycogen-rich cells in the accessory lobes and reveal that these cells are intimately associated with nerve axons. The finding of synaptic complexes and other junctional specializations between nerves and accessory lobe cells suggests that they may have a functional relationship with the nervous system. It is felt that the accessory lobe cells may be neuroglia, possibly of the astrocytic type, which have an innate capacity for glycogen storage. While the functional significance of such glycogen remains obscure, the close morphological association between neurons and the accessory lobe cells enhances the hypothesis put forth by us regarding the glycogen body, namely that neural glycogen is involved in myelin synthesis in the avian nervous system.", "PMID": 978164}
{"id": "pubmed23n0032_11528", "title": "Hypoxia: a succinate-fumerate electron shuttle between peripheral cells and lung.", "content": "The objective of this investigation was to determine if the succinate that accumulates in anoxic or hypoxic tissues of mammals is (a) increased in concentration in the blood, (b) excreted in the urine or (c) recycled in some manner. Rats were subjected to 0.4 atm and the plasma analyzed for succinate and fumarate at 2 hours, 24 hours and 28 days. Significant increases of succinate or succinate plus fumarate were obtained at 2 hours and 28  days at 0.4 atm. Analysis of 24-hour samples of urine obtained from controls and animals at 0.4 atm for 28 days demonstrated that no substantial increase in succinate excretion occurred with acclimation. Lung slices were incubated in Krebs-Ringer bicarbonate and succinate and gassed with O2:CO2 (95:5). Such oxygenated lung slices not only utilized succinate but produced a comparable quantity of fumerate plus malate. It is concluded that succinate produced from fumarate and alpha-ketoglutarate in peripheral hypotic tissue is transported by the blood to the oxygenated lungs. There it is oxidized to fumarate and recycled as fumarate and malate to the periphery. In this way metabolites can act as electron shuttles between peripheral cells and lung.", "contents": "Hypoxia: a succinate-fumerate electron shuttle between peripheral cells and lung. The objective of this investigation was to determine if the succinate that accumulates in anoxic or hypoxic tissues of mammals is (a) increased in concentration in the blood, (b) excreted in the urine or (c) recycled in some manner. Rats were subjected to 0.4 atm and the plasma analyzed for succinate and fumarate at 2 hours, 24 hours and 28 days. Significant increases of succinate or succinate plus fumarate were obtained at 2 hours and 28  days at 0.4 atm. Analysis of 24-hour samples of urine obtained from controls and animals at 0.4 atm for 28 days demonstrated that no substantial increase in succinate excretion occurred with acclimation. Lung slices were incubated in Krebs-Ringer bicarbonate and succinate and gassed with O2:CO2 (95:5). Such oxygenated lung slices not only utilized succinate but produced a comparable quantity of fumerate plus malate. It is concluded that succinate produced from fumarate and alpha-ketoglutarate in peripheral hypotic tissue is transported by the blood to the oxygenated lungs. There it is oxidized to fumarate and recycled as fumarate and malate to the periphery. In this way metabolites can act as electron shuttles between peripheral cells and lung.", "PMID": 978165}
{"id": "pubmed23n0032_11529", "title": "Breakdown of the germinal vesicle in pig oocytes in vivo and in vitro.", "content": "The breakdown of the pig germinal vesicle (GV) was studied in follicular oocytes matured in vivo and in vitro. For better characterization, the whole process was divided into four well-defined stages, based on the chromatin changes, and on nucleolus and nuclear membrane disappearance. In the intact germinal vesicle (GV I) nuclear membrane and nucleolus are clearly visible and chromatin forms a ring or horseshoe around the nucleolus. In the GV II a fre orceinpositive structures (chromocenters) on the nuclear membrane can be detected. For the GV III slightly stained chromatin clumps, localized especially around the nucleolus, and the beginning of strand formation are typical. In the last stage - GV IV - the nuclear membrane is less distinct and the nucleolus disappears completely. Chromatin is seen as an irregular network or as individual bivalents. According to these criteria GV breakdown in vivo was completed in most oocytes between 20 to 24 hours after HCG injection. In culture a similar stage of development was reached between 16 to 20 hours. This difference in the progress of nuclear changes appeared at the beginning of nuclear maturation and remained unchanged throughout the whole period studied.", "contents": "Breakdown of the germinal vesicle in pig oocytes in vivo and in vitro. The breakdown of the pig germinal vesicle (GV) was studied in follicular oocytes matured in vivo and in vitro. For better characterization, the whole process was divided into four well-defined stages, based on the chromatin changes, and on nucleolus and nuclear membrane disappearance. In the intact germinal vesicle (GV I) nuclear membrane and nucleolus are clearly visible and chromatin forms a ring or horseshoe around the nucleolus. In the GV II a fre orceinpositive structures (chromocenters) on the nuclear membrane can be detected. For the GV III slightly stained chromatin clumps, localized especially around the nucleolus, and the beginning of strand formation are typical. In the last stage - GV IV - the nuclear membrane is less distinct and the nucleolus disappears completely. Chromatin is seen as an irregular network or as individual bivalents. According to these criteria GV breakdown in vivo was completed in most oocytes between 20 to 24 hours after HCG injection. In culture a similar stage of development was reached between 16 to 20 hours. This difference in the progress of nuclear changes appeared at the beginning of nuclear maturation and remained unchanged throughout the whole period studied.", "PMID": 978166}
{"id": "pubmed23n0032_11530", "title": "Morphology and kinetics of the hamster sperm acrosome reaction.", "content": "The morphology and kinetics of the normal acrosome reaction were examined in vitro using hamster sperm incubated in detoxified sera. The reaction involved either swelling and elevation or crenulation and fragmentation of the acrosomal cap. Swelling and elevation occurred during both normal and degenerative reactions, as reported by others. Crenulation with subsequent fragmentation of the cap was observed during normal reactions. Early crenulation of the acrosome could be induced by cold shock (5 degrees C, 25 minutes), but this did not decrease the incubation time required (at 37 degrees C) for completion of the normal reaction. In appropriate sera, the occurrence of normal and degenerative acrosome reactions in motile sperm was significantly separated in time to study the reactions independently. The duration of the normal reaction, i.e., the time between the first morphological change in the acrosome (initiation)until the actual detachment of the cap (termination) was estimated to be 20 minutes. Saline dilution of these sera delayed initiation of the reaction and increased the duration of the reaction  once it had started. Data from cold-shock and serum dilution experiments indicate that the mechanisms which govern the initiation and termination of the normal reaction are independently variable, and further suggest that initiation involves a change in membrane permeability and that termination includes membrane vesiculation.", "contents": "Morphology and kinetics of the hamster sperm acrosome reaction. The morphology and kinetics of the normal acrosome reaction were examined in vitro using hamster sperm incubated in detoxified sera. The reaction involved either swelling and elevation or crenulation and fragmentation of the acrosomal cap. Swelling and elevation occurred during both normal and degenerative reactions, as reported by others. Crenulation with subsequent fragmentation of the cap was observed during normal reactions. Early crenulation of the acrosome could be induced by cold shock (5 degrees C, 25 minutes), but this did not decrease the incubation time required (at 37 degrees C) for completion of the normal reaction. In appropriate sera, the occurrence of normal and degenerative acrosome reactions in motile sperm was significantly separated in time to study the reactions independently. The duration of the normal reaction, i.e., the time between the first morphological change in the acrosome (initiation)until the actual detachment of the cap (termination) was estimated to be 20 minutes. Saline dilution of these sera delayed initiation of the reaction and increased the duration of the reaction  once it had started. Data from cold-shock and serum dilution experiments indicate that the mechanisms which govern the initiation and termination of the normal reaction are independently variable, and further suggest that initiation involves a change in membrane permeability and that termination includes membrane vesiculation.", "PMID": 978167}
{"id": "pubmed23n0032_11531", "title": "The development of the oxygen permeability of the avian egg shell and its membranes during incubation.", "content": "The 02-permeability of the avian egg shell and shell membranes is initially low (approximately equal to 0.1-10(-6) ml O2 STP-sec-1cm-2-mmHg-1) during incubation but increases about 10-fold after the first week. This increase correlates with a decline in water content of the membranes. A major increase in colloid osmotic pressure (up to 50 cm Hg) occurs and is the suggested cause for the onset of rapid removal of water from the membranes. A high membrane water content correlates with low O2-permeability and vice versa. The final degree of membrane hydration reached during incubation is independent  of ambient humidity; that is, the O2-permeability of the shell and its membranes is controlled by conditions inside the egg. An hypothesis is presented to explain the profound increase in O2-permeability while the rate of water loss from the egg remains stable during incubation. The removal of water from the shell membranes by the increased colloid osmotic pressure will increase the number of gas filled channels in the membranes which in turn will increase the O2-permeability. In spite of the change in water content, the water vapor pressure in the shell membranes will always be very close to that of a free water surface and water loss will hence be determined by the ambient humidity and the porosity of the mineral shell.", "contents": "The development of the oxygen permeability of the avian egg shell and its membranes during incubation. The 02-permeability of the avian egg shell and shell membranes is initially low (approximately equal to 0.1-10(-6) ml O2 STP-sec-1cm-2-mmHg-1) during incubation but increases about 10-fold after the first week. This increase correlates with a decline in water content of the membranes. A major increase in colloid osmotic pressure (up to 50 cm Hg) occurs and is the suggested cause for the onset of rapid removal of water from the membranes. A high membrane water content correlates with low O2-permeability and vice versa. The final degree of membrane hydration reached during incubation is independent  of ambient humidity; that is, the O2-permeability of the shell and its membranes is controlled by conditions inside the egg. An hypothesis is presented to explain the profound increase in O2-permeability while the rate of water loss from the egg remains stable during incubation. The removal of water from the shell membranes by the increased colloid osmotic pressure will increase the number of gas filled channels in the membranes which in turn will increase the O2-permeability. In spite of the change in water content, the water vapor pressure in the shell membranes will always be very close to that of a free water surface and water loss will hence be determined by the ambient humidity and the porosity of the mineral shell.", "PMID": 978168}
{"id": "pubmed23n0032_11532", "title": "Epithelial stratification in the developing chick cornea.", "content": "The process of epithelial stratification was studied in the embryonic chick corneal eipthelium between 10 and 21 days of incubation. Information was collected on the DNA synthetic activity occurring in individual cell layers, on cell density changes in the basal layer, and on mitotic spindle orientation in each cell layer before, during, and upon the completion of alyer formation. Prior  to the initiation of stratification, mitotic spindles are oriented parallel to the basement membrane interface. This orientation changes to predominantly vertically directed spindles as layer formation proceeds. Later, the majority of the spindles are horizontally aligned once more. This pattern was observed as each successive cell layer formed. The possible relationship between the spindle data and that obtained on DNA synthetic activity and cell density changes is discussed in terms of the role these factors might play in layer formation in the cornea, as well as in other stratified epithelia.", "contents": "Epithelial stratification in the developing chick cornea. The process of epithelial stratification was studied in the embryonic chick corneal eipthelium between 10 and 21 days of incubation. Information was collected on the DNA synthetic activity occurring in individual cell layers, on cell density changes in the basal layer, and on mitotic spindle orientation in each cell layer before, during, and upon the completion of alyer formation. Prior  to the initiation of stratification, mitotic spindles are oriented parallel to the basement membrane interface. This orientation changes to predominantly vertically directed spindles as layer formation proceeds. Later, the majority of the spindles are horizontally aligned once more. This pattern was observed as each successive cell layer formed. The possible relationship between the spindle data and that obtained on DNA synthetic activity and cell density changes is discussed in terms of the role these factors might play in layer formation in the cornea, as well as in other stratified epithelia.", "PMID": 978169}
{"id": "pubmed23n0032_11533", "title": "DNA synthesis during the development of the chick cornea.", "content": "The frequency and pattern of DNA synthesis were analysed autoradiographically in the developing chick cornea. Each cellular population was studied in time-sequence fashion from the time of its appearance until hatching. There is a sharp drop in the synthetic index (number of labeled cells/total number of cells) in the anterior corneal epithelium soon after its formation, corresponding in time to the secretion of extracellular matrix material by this tissue. A similar decrease does not occur in adjacent tissues. Continous labeling experiments show that about 20% of the corneal cells are not in the proliferative pool at this time while 100% of the cells in the underlying lens epithelium and the surrounding head epidermis and head mesenchyme are labeled. Cell cycle measurements indicate that the proliferative kinetics of both the corneal epithelium and the head epidermis are similar at this time even though the percentage of labeled cells in each region differs. The formation of the corneal endothelium and the movement of fibroblasts into the stromal region are events which involve extensive cellular migration. Labeled cells are observed at all stages of both endothelial and stromal fibroblast migration, indicating that DNA synthesis occurs during the course of cellular migration in the developing cornea.", "contents": "DNA synthesis during the development of the chick cornea. The frequency and pattern of DNA synthesis were analysed autoradiographically in the developing chick cornea. Each cellular population was studied in time-sequence fashion from the time of its appearance until hatching. There is a sharp drop in the synthetic index (number of labeled cells/total number of cells) in the anterior corneal epithelium soon after its formation, corresponding in time to the secretion of extracellular matrix material by this tissue. A similar decrease does not occur in adjacent tissues. Continous labeling experiments show that about 20% of the corneal cells are not in the proliferative pool at this time while 100% of the cells in the underlying lens epithelium and the surrounding head epidermis and head mesenchyme are labeled. Cell cycle measurements indicate that the proliferative kinetics of both the corneal epithelium and the head epidermis are similar at this time even though the percentage of labeled cells in each region differs. The formation of the corneal endothelium and the movement of fibroblasts into the stromal region are events which involve extensive cellular migration. Labeled cells are observed at all stages of both endothelial and stromal fibroblast migration, indicating that DNA synthesis occurs during the course of cellular migration in the developing cornea.", "PMID": 978170}
{"id": "pubmed23n0032_11534", "title": "Cytophotometric study of nuclear proteins during embryogenesis in two nematode species, Ascaris lumbricoides and Panagrellus silusiae.", "content": "Patterns of nuclear protein changes during embryogenesis in two nematode systems: Ascaris lumbricoides and Panagrellus silusiae were examined microspectrophotometrically. In both species, development is accompanied by an extensive loss of basic nuclear protein as noted by the diminished intranuclear binding of both the Sakaguchi reagent and fluorodinitrobenzene chromophore. The alkaline fast green (pH 8.1) procedure stained both nuclear and cytoplasmic regions of interphase cells to a similar extent throughout embryogenesis. In cells at metaphase the chromosomes bound the fast green dye intensely while the cytoplasm continued to give a positive reaction. Relative Feulgen stainability of nuclei during embryonic development varied between species. In Ascaris, the one-celled stage nucleus was Feulgen-negative and the LI juvenile somatic nuclei yielded a mean Feulgen-DNA value which was considerably less than a postdiminution diploid DNA equivilent. In Panagrellus, the nuclei of one-celled stage embryos contain DNA in excess of a 4C quantity which subsequently is reduced during embryonic divisions. As in Ascaris the nuclei of Panagrellus LI juveniles give hypodiploid Feulgen-DNA values. The significance of these changes remains to be determined.", "contents": "Cytophotometric study of nuclear proteins during embryogenesis in two nematode species, Ascaris lumbricoides and Panagrellus silusiae. Patterns of nuclear protein changes during embryogenesis in two nematode systems: Ascaris lumbricoides and Panagrellus silusiae were examined microspectrophotometrically. In both species, development is accompanied by an extensive loss of basic nuclear protein as noted by the diminished intranuclear binding of both the Sakaguchi reagent and fluorodinitrobenzene chromophore. The alkaline fast green (pH 8.1) procedure stained both nuclear and cytoplasmic regions of interphase cells to a similar extent throughout embryogenesis. In cells at metaphase the chromosomes bound the fast green dye intensely while the cytoplasm continued to give a positive reaction. Relative Feulgen stainability of nuclei during embryonic development varied between species. In Ascaris, the one-celled stage nucleus was Feulgen-negative and the LI juvenile somatic nuclei yielded a mean Feulgen-DNA value which was considerably less than a postdiminution diploid DNA equivilent. In Panagrellus, the nuclei of one-celled stage embryos contain DNA in excess of a 4C quantity which subsequently is reduced during embryonic divisions. As in Ascaris the nuclei of Panagrellus LI juveniles give hypodiploid Feulgen-DNA values. The significance of these changes remains to be determined.", "PMID": 978171}
{"id": "pubmed23n0032_11535", "title": "Cytophotometric analysis of basic nuclear proteins during gametogenesis in the free-living nematode Panagrellus silusiae.", "content": "The cytochemical staining characteristics of basic nuclear proteins during gametogenesis in both sexes of the free-living nematode Panagrellus silusiae were monitored by absorption microspectrophotometry. During spermatogenesis, the levels of both of the nuclear-bound Sakaguchi and 1-fluoro-2, 4-dinitrobenzene (FDNB) reagents per Feulgen-DNA content increase prior to meiosis and subsequently drop off during spermiogenesis. During oogenesis, the amount of Sakaguchi-protein to DNA ratio rises slightly whereas the FDNB-protein to DNA ratio undergoes a meagre decline. Since the oocyte of Panagrellus accumulates DNA in excess of a 4C DNA equivalent, the relative constancy of the ratio of basic nuclear protein content to DNA amount during oogenesis signifies that these proteins are being continously produced during oocyte maturation. After treatment with alkaline fast green (pH 8.1) the cytoplasmic and nuclear regions of the gonocytes of both sexes yield such an intense reaction that microspectrophotometric measurements could not be taken. The basis of the cytoplasmic staining reaction to the fast green (pH 8.1) dye was not determined. The rapid, highly deterministic programme of embryogenesis in Panagrellus may require a substantial stockpiling of basic nuclear proteins during oogenesis.", "contents": "Cytophotometric analysis of basic nuclear proteins during gametogenesis in the free-living nematode Panagrellus silusiae. The cytochemical staining characteristics of basic nuclear proteins during gametogenesis in both sexes of the free-living nematode Panagrellus silusiae were monitored by absorption microspectrophotometry. During spermatogenesis, the levels of both of the nuclear-bound Sakaguchi and 1-fluoro-2, 4-dinitrobenzene (FDNB) reagents per Feulgen-DNA content increase prior to meiosis and subsequently drop off during spermiogenesis. During oogenesis, the amount of Sakaguchi-protein to DNA ratio rises slightly whereas the FDNB-protein to DNA ratio undergoes a meagre decline. Since the oocyte of Panagrellus accumulates DNA in excess of a 4C DNA equivalent, the relative constancy of the ratio of basic nuclear protein content to DNA amount during oogenesis signifies that these proteins are being continously produced during oocyte maturation. After treatment with alkaline fast green (pH 8.1) the cytoplasmic and nuclear regions of the gonocytes of both sexes yield such an intense reaction that microspectrophotometric measurements could not be taken. The basis of the cytoplasmic staining reaction to the fast green (pH 8.1) dye was not determined. The rapid, highly deterministic programme of embryogenesis in Panagrellus may require a substantial stockpiling of basic nuclear proteins during oogenesis.", "PMID": 978172}
{"id": "pubmed23n0032_11536", "title": "Testis specific phosphoglycerate kinase B in mouse.", "content": "Mammals possess two genetically distinct forms of phosphoglycerate kinase (PGK). Only the X-linked PGK-A isozyme is detectable by electrophoresis of somatic and ovarian tissues of the mouse and of most other eutherian mammals studied to date. The PGK-B isozyme is highly active in testes and epididymides which contain spermatozoa, as well as in semen. PGK-B activity is first detectable in testes of 22-day-old mice and shows rapid increase concomitant with germ cell proliferation until day 40,after which activity increases only slightly. Two allelic isozymes (F and S) in mice express equal enzyme activity and are inherited in an autosomal codominant manner. One is equally as heat stable as PGK-A; the other is heat labile. Two of the 33 strains tested express neither F nor S, but do express a faintly staining PGK band which is of slightly faster mobility than F and which is absent from the other stains. This may represent a third allelic isozyme of PGK-B with reduced enzyme activity.", "contents": "Testis specific phosphoglycerate kinase B in mouse. Mammals possess two genetically distinct forms of phosphoglycerate kinase (PGK). Only the X-linked PGK-A isozyme is detectable by electrophoresis of somatic and ovarian tissues of the mouse and of most other eutherian mammals studied to date. The PGK-B isozyme is highly active in testes and epididymides which contain spermatozoa, as well as in semen. PGK-B activity is first detectable in testes of 22-day-old mice and shows rapid increase concomitant with germ cell proliferation until day 40,after which activity increases only slightly. Two allelic isozymes (F and S) in mice express equal enzyme activity and are inherited in an autosomal codominant manner. One is equally as heat stable as PGK-A; the other is heat labile. Two of the 33 strains tested express neither F nor S, but do express a faintly staining PGK band which is of slightly faster mobility than F and which is absent from the other stains. This may represent a third allelic isozyme of PGK-B with reduced enzyme activity.", "PMID": 978173}
{"id": "pubmed23n0032_11537", "title": "X-ray induced inhibition of DNA synthesis and mitosis in internal tissues during the initiation of limb regeneration in the adult newt.", "content": "Left front limbs of adult male newts were given 2000r of X-irradiation at least four weeks prior to amputation of both forelimbs. Internal stump tissues were evaluated for the ability to incorporate 3H-thymidine and accumulate colchicine-blocked mitotic figures. In otherwise uninjured limbs, irradiation stimulated low levels of DNA synthesis which did not increase significantly after amputation. Thus, as soon as DNA synthesis increased significantly in normal limbs as a result of amputation, it was demonstrably higher than in X-rayed limbs. In general, mitotic activity in both groups reflected the DNA synthetic rates. Since others have shown that denervation at the time of amputation blocks subsequent mitosis in internal stump tissues yet allows normal levels of DNA synthesis for eight days, we conclude that X-irradiation and denervation prevent cell division in potential blastema cells by different mechanisms.", "contents": "X-ray induced inhibition of DNA synthesis and mitosis in internal tissues during the initiation of limb regeneration in the adult newt. Left front limbs of adult male newts were given 2000r of X-irradiation at least four weeks prior to amputation of both forelimbs. Internal stump tissues were evaluated for the ability to incorporate 3H-thymidine and accumulate colchicine-blocked mitotic figures. In otherwise uninjured limbs, irradiation stimulated low levels of DNA synthesis which did not increase significantly after amputation. Thus, as soon as DNA synthesis increased significantly in normal limbs as a result of amputation, it was demonstrably higher than in X-rayed limbs. In general, mitotic activity in both groups reflected the DNA synthetic rates. Since others have shown that denervation at the time of amputation blocks subsequent mitosis in internal stump tissues yet allows normal levels of DNA synthesis for eight days, we conclude that X-irradiation and denervation prevent cell division in potential blastema cells by different mechanisms.", "PMID": 978174}
{"id": "pubmed23n0032_11538", "title": "The role of extracellular material in chick neurulation. I. Effects of concanavalin A.", "content": "The distribution of extracellular material (ECM) in developing neuroepithelium of stage 8+ chick embryos was investigated using lanthanum nitrate. The temporal changes in the distribution of ECM was correlated with the ongoing morphogenetic movements. The most striking finding was that as the folds were about to meet, thick dense ECM appeared between leading edges. Concanavalin A inhibited neural tube closure by binding to cell surfaces and disrupting the usual distribution of ECM in developing neuroepithelium.", "contents": "The role of extracellular material in chick neurulation. I. Effects of concanavalin A. The distribution of extracellular material (ECM) in developing neuroepithelium of stage 8+ chick embryos was investigated using lanthanum nitrate. The temporal changes in the distribution of ECM was correlated with the ongoing morphogenetic movements. The most striking finding was that as the folds were about to meet, thick dense ECM appeared between leading edges. Concanavalin A inhibited neural tube closure by binding to cell surfaces and disrupting the usual distribution of ECM in developing neuroepithelium.", "PMID": 978175}
{"id": "pubmed23n0032_11539", "title": "Male haploidy in rotifers: relative DNA content of nuclei from male and female Asplanchna.", "content": "In A. sieboldi mitotic nuclei of males had one-half the nuclear DNA content of those of females. Spermatocytes from testes had 1c DNA contents. These observations indicate that the male is haploid and that spermatogenesis is atypical and involves no reductional divisions.", "contents": "Male haploidy in rotifers: relative DNA content of nuclei from male and female Asplanchna. In A. sieboldi mitotic nuclei of males had one-half the nuclear DNA content of those of females. Spermatocytes from testes had 1c DNA contents. These observations indicate that the male is haploid and that spermatogenesis is atypical and involves no reductional divisions.", "PMID": 978176}
{"id": "pubmed23n0032_11540", "title": "Isolation and characterization of Agrobacterium tumefaciens mutants affected in the utilization of octopine, octopinic acid and lysopine.", "content": "Using an enrichment procedure, mutant strains of Agrobacterium tumefaciens were isolated that lacked the ability to utilize octopine as a nitrogen source. Of 55 such isolates, 44 were unable to utilize several amino acids; the remaining 11 strains were altered solely in their ability to utilize octopine, octopinic acid and lysopine. It is concluded that only the latter were plasmid mutations. Among them, there was a high, but no absolute, correlation with avirulence. All strains contained the T1 plasmid. All virulent strains showed active transport of octopine when they had previously been grown in medium containing octopine, whereas the avirulent strains failed to show such transport. All the virulent mutants induced tumours containing octopine. The results are discussed in relation to the hypothesis that the genes which code for the octopine synthesizing enzymes in the tumour are of bacterial origin.", "contents": "Isolation and characterization of Agrobacterium tumefaciens mutants affected in the utilization of octopine, octopinic acid and lysopine. Using an enrichment procedure, mutant strains of Agrobacterium tumefaciens were isolated that lacked the ability to utilize octopine as a nitrogen source. Of 55 such isolates, 44 were unable to utilize several amino acids; the remaining 11 strains were altered solely in their ability to utilize octopine, octopinic acid and lysopine. It is concluded that only the latter were plasmid mutations. Among them, there was a high, but no absolute, correlation with avirulence. All strains contained the T1 plasmid. All virulent strains showed active transport of octopine when they had previously been grown in medium containing octopine, whereas the avirulent strains failed to show such transport. All the virulent mutants induced tumours containing octopine. The results are discussed in relation to the hypothesis that the genes which code for the octopine synthesizing enzymes in the tumour are of bacterial origin.", "PMID": 978177}
{"id": "pubmed23n0032_11541", "title": "Sodium-dependent growth and respiration of a nonhalophilic bacterium, Pseudomonas stutzeri.", "content": "Pseudomonas stutzeri (van Niel strain) requires Na+ for growth. Its growth rate was a sigmoidal function of Na+ concentration, being maximal and constant from 2 to 50 mM-Na+, and half maximal at about 0-5 mM-Na+. The relationship between cell concentration and Na+ concentration was non-linear; cell concentration increased abruptly when Na+ was greater than 0-3 mM. Accumulation of Na+ in the organism during growth was not detected. In the presence of K+, respiration was enhanced specifically by Na+. The respiration rate of the organism growing in the culture was a linear function of the growth rate when limited by the Na+ concentration, whereas the maximum rate induced by excess Na+ was independent of the growth rate.", "contents": "Sodium-dependent growth and respiration of a nonhalophilic bacterium, Pseudomonas stutzeri. Pseudomonas stutzeri (van Niel strain) requires Na+ for growth. Its growth rate was a sigmoidal function of Na+ concentration, being maximal and constant from 2 to 50 mM-Na+, and half maximal at about 0-5 mM-Na+. The relationship between cell concentration and Na+ concentration was non-linear; cell concentration increased abruptly when Na+ was greater than 0-3 mM. Accumulation of Na+ in the organism during growth was not detected. In the presence of K+, respiration was enhanced specifically by Na+. The respiration rate of the organism growing in the culture was a linear function of the growth rate when limited by the Na+ concentration, whereas the maximum rate induced by excess Na+ was independent of the growth rate.", "PMID": 978178}
{"id": "pubmed23n0032_11542", "title": "Cell-wall composition and structure of yeast cells and conjugation tubes of Tremella mesenterica.", "content": "Cell walls prepared from vegetative yeast cells and from hormone-induced conjugation tubes of the basidiomycete Tremella mesenterica had similar compositions. Evidence was found for 1,3-alpha-glucan (yeast 38%, tube 25%), 1,3-beta-1,6-beta-glucan (yeast 33%, tube 48%) and chitin (both less than 3%) in the walls. The walls also contained xylose (5 to 7%), mannose (6%), glucuronic acid (approx. 2%), and traces of galactose. Protein amounted to less than 2% of the wall weight. The cell capsule was very insoluble and could not be removed from the cell wall. The conjugation hormone did not appear to exert its effect on cell shape by causing gross changes in wall composition.", "contents": "Cell-wall composition and structure of yeast cells and conjugation tubes of Tremella mesenterica. Cell walls prepared from vegetative yeast cells and from hormone-induced conjugation tubes of the basidiomycete Tremella mesenterica had similar compositions. Evidence was found for 1,3-alpha-glucan (yeast 38%, tube 25%), 1,3-beta-1,6-beta-glucan (yeast 33%, tube 48%) and chitin (both less than 3%) in the walls. The walls also contained xylose (5 to 7%), mannose (6%), glucuronic acid (approx. 2%), and traces of galactose. Protein amounted to less than 2% of the wall weight. The cell capsule was very insoluble and could not be removed from the cell wall. The conjugation hormone did not appear to exert its effect on cell shape by causing gross changes in wall composition.", "PMID": 978180}
{"id": "pubmed23n0032_11543", "title": "Polyamines in bacteriophage phi W-14 and in phi W-14-infected Pseudomonas acidovorans.", "content": "Bacteriophage phi W-14 is sensitive to osmotic shock. It contain sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the DNA phosphates. The alpha-putrescinylthymine residues of the DNA could neutralize a further 25% of the phosphates. Label is transferred from ornithine to the alpha-putrescinyl residues of phi W-14 DNA. The rates of polyamine synthesis in Pseudomonas acidovorans are increased by phi W-14 infection.", "contents": "Polyamines in bacteriophage phi W-14 and in phi W-14-infected Pseudomonas acidovorans. Bacteriophage phi W-14 is sensitive to osmotic shock. It contain sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the DNA phosphates. The alpha-putrescinylthymine residues of the DNA could neutralize a further 25% of the phosphates. Label is transferred from ornithine to the alpha-putrescinyl residues of phi W-14 DNA. The rates of polyamine synthesis in Pseudomonas acidovorans are increased by phi W-14 infection.", "PMID": 978181}
{"id": "pubmed23n0032_11544", "title": "Salt-dependent haemagglutinating measles virus in S.S.P.E.", "content": "Two strains fo S.S.P.E. virus show both haemagglutinin and salt-dependent haemagglutinin. These properties are associated with distinct plaque forms within each strain. The existence of salt-dependent strains in wild-type measles virus suggests that they should be found in the early isolates of all strains of S.S.P.E.", "contents": "Salt-dependent haemagglutinating measles virus in S.S.P.E. Two strains fo S.S.P.E. virus show both haemagglutinin and salt-dependent haemagglutinin. These properties are associated with distinct plaque forms within each strain. The existence of salt-dependent strains in wild-type measles virus suggests that they should be found in the early isolates of all strains of S.S.P.E.", "PMID": 978182}
{"id": "pubmed23n0032_11545", "title": "Inhibition of influenza virus replication in tissue culture by 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA): mechanism of action.", "content": "The neuraminidase inhibitor 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA) inhibits the mutlicycle replication of influenza viruses in tissue culture. Influenza virus grown in the presence of FANA contains neuraminic acid on its envelope which then serves as receptor for other virus particles causing extensive aggregation. Thus, FANA inhibits influenza virus replication by preventing the enzymatic removal of neuraminic acid from the virus envelope.", "contents": "Inhibition of influenza virus replication in tissue culture by 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA): mechanism of action. The neuraminidase inhibitor 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA) inhibits the mutlicycle replication of influenza viruses in tissue culture. Influenza virus grown in the presence of FANA contains neuraminic acid on its envelope which then serves as receptor for other virus particles causing extensive aggregation. Thus, FANA inhibits influenza virus replication by preventing the enzymatic removal of neuraminic acid from the virus envelope.", "PMID": 978183}
{"id": "pubmed23n0032_11546", "title": "Effects of interferon on the human clonal cell line, RSa: inhibition of macromolecular synthesis.", "content": "Multiplication of the human clonal cell line, RSa, is completely inhibited by human leukocyte interferon preparations. Synthesis of DNA and protein is markedly reduced in these cells in proportion to the concentration of interferon applied. Interferon treatment leads to accumulation of cells of an epithelial morphology which do not enter the division cycle. It is suggested that the growth inhibitory effects of interferon on RSa cells may result from these effects.", "contents": "Effects of interferon on the human clonal cell line, RSa: inhibition of macromolecular synthesis. Multiplication of the human clonal cell line, RSa, is completely inhibited by human leukocyte interferon preparations. Synthesis of DNA and protein is markedly reduced in these cells in proportion to the concentration of interferon applied. Interferon treatment leads to accumulation of cells of an epithelial morphology which do not enter the division cycle. It is suggested that the growth inhibitory effects of interferon on RSa cells may result from these effects.", "PMID": 978184}
{"id": "pubmed23n0032_11547", "title": "A small RNA virus with a divided genome from Heteronychus arator (F.) [Coleoperai Scarabaeidae].", "content": "Some properties of a small RNA virus isolated from larvae of Heteronychus arator [Coleoptera: Scarabaeidae] are described. The virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. The sedimentation coefficient of virus was 137S and the buoyant density was 1-33 g/ml. The single stranded RNA was isolated as two species which sedimented at 22S and 15S, corresponding to mol. wt. of 1-0 and 0-5 X 10(6) respectively. The base ratios of the two RNA species were 22 S: A=29-0, C=24-1, G\u00bf-8, U=24-1; 15S:A=26-4, C=23-8, G=24-7, U=25-2. There was one major polypeptide, mol. wt. 40 000 and two minor polypeptides in the virus. The virus was infective for Galleria mellonella and some other insects, but was not infective for mice. No evidence was obtained to suggest that the two RNA species were present in different particles.", "contents": "A small RNA virus with a divided genome from Heteronychus arator (F.) [Coleoperai Scarabaeidae]. Some properties of a small RNA virus isolated from larvae of Heteronychus arator [Coleoptera: Scarabaeidae] are described. The virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. The sedimentation coefficient of virus was 137S and the buoyant density was 1-33 g/ml. The single stranded RNA was isolated as two species which sedimented at 22S and 15S, corresponding to mol. wt. of 1-0 and 0-5 X 10(6) respectively. The base ratios of the two RNA species were 22 S: A=29-0, C=24-1, G\u00bf-8, U=24-1; 15S:A=26-4, C=23-8, G=24-7, U=25-2. There was one major polypeptide, mol. wt. 40 000 and two minor polypeptides in the virus. The virus was infective for Galleria mellonella and some other insects, but was not infective for mice. No evidence was obtained to suggest that the two RNA species were present in different particles.", "PMID": 978185}
{"id": "pubmed23n0032_11548", "title": "Recent life changes and outcome of prolonged competitive stress.", "content": "To test the predictive utility of subjects' recent life changes with regard to outcome of prolonged competitive stress, the authors studied racers (N = 25) in a long distance Alaskan sled race, the Iditarod. Rank order correlations between subjects' 1-year life change units (LCU) total and adjusted place of finish proved significant by both Kendall's and Spearman's Test. Thus, lowe LCU scores correlated with better performance and higher scores with poorer performance. Differences between subgroupings of the racers are considered. The work suggests that the usefulness of recent life changes assessment may be extended beyong physical illness, particularly in the study of response and adaptation to prolonged stress.", "contents": "Recent life changes and outcome of prolonged competitive stress. To test the predictive utility of subjects' recent life changes with regard to outcome of prolonged competitive stress, the authors studied racers (N = 25) in a long distance Alaskan sled race, the Iditarod. Rank order correlations between subjects' 1-year life change units (LCU) total and adjusted place of finish proved significant by both Kendall's and Spearman's Test. Thus, lowe LCU scores correlated with better performance and higher scores with poorer performance. Differences between subgroupings of the racers are considered. The work suggests that the usefulness of recent life changes assessment may be extended beyong physical illness, particularly in the study of response and adaptation to prolonged stress.", "PMID": 978186}
{"id": "pubmed23n0032_11549", "title": "On the methods and theory of reliability.", "content": "This paper reviews the most frequently used and misused reliability measures appearing in the mental health literature. We illustrate the various types of data sets on which reliability is assessed (i.e., two raters, more than two raters, and varying numbers of raters with dichotomous, polychotomous, and quantitative data). Reliability statistics appropriate for each data format are presented, and their pros and cons illustrated. Inadequancies of some methods are highlighted. The meaning of different levels of reliability obtained with various statistics is discussed. This critique is intended for the reading professional and the investigator who has an occasional need for reliability assessment. Statistical expertise is not required and theoretical material is referenced for the interested reader. Necessary formulas for computations are presented in the appendices. A summary table of some suitable reliability measures is presented.", "contents": "On the methods and theory of reliability. This paper reviews the most frequently used and misused reliability measures appearing in the mental health literature. We illustrate the various types of data sets on which reliability is assessed (i.e., two raters, more than two raters, and varying numbers of raters with dichotomous, polychotomous, and quantitative data). Reliability statistics appropriate for each data format are presented, and their pros and cons illustrated. Inadequancies of some methods are highlighted. The meaning of different levels of reliability obtained with various statistics is discussed. This critique is intended for the reading professional and the investigator who has an occasional need for reliability assessment. Statistical expertise is not required and theoretical material is referenced for the interested reader. Necessary formulas for computations are presented in the appendices. A summary table of some suitable reliability measures is presented.", "PMID": 978187}
{"id": "pubmed23n0032_11550", "title": "Psychological differentiation and psychotherapy.", "content": "Patient's level of psychological differentiation was studied in relation to patients' and therapists' evaluations of the initial psychotherapy session. The evaluations assessed two broad dimensions of the interaction which generally have been considered important for psychotherapeutic success and which have been found to be related to patients' psychological differentiation: namely, patients' and therapists' satisfaction with the amount of structure, and therapists' impressions of patients' appropriateness for therapy. Significant correlations were obtained between patients' psychological differentiation and both of these dimensions. However, two control variables, socioeconomic status and alcohol use, were also found to be related to these dimensions. When the effects of these variables were partialed out, the relationships with therapists' impressions of appropriateness disappeared, but the relationships with patients' and therapists' satisfaction with the amount of structure remained significant. The relationship of these findings to other studies of psychological differentiation and psychotherapy is discussed. Finally, the contribution of the present results to the choice of the most appropriate measure of psychological differentiation is noted.", "contents": "Psychological differentiation and psychotherapy. Patient's level of psychological differentiation was studied in relation to patients' and therapists' evaluations of the initial psychotherapy session. The evaluations assessed two broad dimensions of the interaction which generally have been considered important for psychotherapeutic success and which have been found to be related to patients' psychological differentiation: namely, patients' and therapists' satisfaction with the amount of structure, and therapists' impressions of patients' appropriateness for therapy. Significant correlations were obtained between patients' psychological differentiation and both of these dimensions. However, two control variables, socioeconomic status and alcohol use, were also found to be related to these dimensions. When the effects of these variables were partialed out, the relationships with therapists' impressions of appropriateness disappeared, but the relationships with patients' and therapists' satisfaction with the amount of structure remained significant. The relationship of these findings to other studies of psychological differentiation and psychotherapy is discussed. Finally, the contribution of the present results to the choice of the most appropriate measure of psychological differentiation is noted.", "PMID": 978188}
{"id": "pubmed23n0032_11551", "title": "The relationships of the process/reactive, paranoid/nonparanoid, length of illness, and length of hospitalization dimensions to schizophrenic abstract thinking deficits.", "content": "Attempts to determine whether the quality of schizophrenic thought disorder varies from one type of schizophrenic to another in earlier studies have been hindered by the contamination of the major individual difference dimensions with each other. In the project described here, partial correlations were run between each of two abstraction deficit measures (Proverbs Test Abstract Level and Autism scores) and four independent variables which represent dimensions commonly used as criteria in individual differences research in schizophrenia--Ullmann-Giovannoni Process-Reactive scores, the MMPI Paranoia Scale, length of illness, and length of hospitalization. In each correlation the three noncriterion individual difference measures, the unused abstraction measure, age, education, and WAIS Vocabulary were partialed out. The correlations were also corrected for attenuation. After partialing, level of abstraction was negatively correlated with processness. Autism was positively related to reactiveness and length of hospitalization. Neither of two paranoid/nonparanoid measures used nor length of illness was related to either dependent variable.", "contents": "The relationships of the process/reactive, paranoid/nonparanoid, length of illness, and length of hospitalization dimensions to schizophrenic abstract thinking deficits. Attempts to determine whether the quality of schizophrenic thought disorder varies from one type of schizophrenic to another in earlier studies have been hindered by the contamination of the major individual difference dimensions with each other. In the project described here, partial correlations were run between each of two abstraction deficit measures (Proverbs Test Abstract Level and Autism scores) and four independent variables which represent dimensions commonly used as criteria in individual differences research in schizophrenia--Ullmann-Giovannoni Process-Reactive scores, the MMPI Paranoia Scale, length of illness, and length of hospitalization. In each correlation the three noncriterion individual difference measures, the unused abstraction measure, age, education, and WAIS Vocabulary were partialed out. The correlations were also corrected for attenuation. After partialing, level of abstraction was negatively correlated with processness. Autism was positively related to reactiveness and length of hospitalization. Neither of two paranoid/nonparanoid measures used nor length of illness was related to either dependent variable.", "PMID": 978189}
{"id": "pubmed23n0032_11552", "title": "Factors compelling psychiatric hospitalization in crisis.", "content": "At a time in a large community mental health center when all adjunct facilities were geared for crises, the compelling determinants in those patients still requiring hospitalization were studied. It was found that all patients answered affirmatively to one or more of the following six categories as occurring in their prehospital life: 1) alcohol abuse; 2) other drug abuse; 3) suicidal attempt; 4) a recent loss; 5) stoppage (or reduction) of medication; 6) stoppage of outpatient treatment. It was found that those patients diagnosed schizophrenic (69 per cent) did not significantly differ in the number of affirmative answers given in each of the six categories when compared to the total patient population. The most frequent categories to which an affirmative answer was given were stoppage of medication and stoppage of outpatient treatment. There did appear to be a trend for various combinations of these categories in a give patients. The schizophrenic group tended to have more affirmative answers in combinations of categories 3 and 4. The importance of these findings in the hospitalization and follow-up treatment of psychiatric patients is discussed.", "contents": "Factors compelling psychiatric hospitalization in crisis. At a time in a large community mental health center when all adjunct facilities were geared for crises, the compelling determinants in those patients still requiring hospitalization were studied. It was found that all patients answered affirmatively to one or more of the following six categories as occurring in their prehospital life: 1) alcohol abuse; 2) other drug abuse; 3) suicidal attempt; 4) a recent loss; 5) stoppage (or reduction) of medication; 6) stoppage of outpatient treatment. It was found that those patients diagnosed schizophrenic (69 per cent) did not significantly differ in the number of affirmative answers given in each of the six categories when compared to the total patient population. The most frequent categories to which an affirmative answer was given were stoppage of medication and stoppage of outpatient treatment. There did appear to be a trend for various combinations of these categories in a give patients. The schizophrenic group tended to have more affirmative answers in combinations of categories 3 and 4. The importance of these findings in the hospitalization and follow-up treatment of psychiatric patients is discussed.", "PMID": 978190}
{"id": "pubmed23n0032_11553", "title": "Prolonged LSD flashbacks as conversion reactions.", "content": "This paper presents a case study of the background and treatment of a patient with prolonged LSD flashbacks. The hypothesis that flashbacks can be psychologically determined symptoms is supported by the dynamics of the case and the course of treatment. A second focus is a partial explanation for the often made observation that obessive-compulsive personalities are at increased risk for LSD flashbacks.", "contents": "Prolonged LSD flashbacks as conversion reactions. This paper presents a case study of the background and treatment of a patient with prolonged LSD flashbacks. The hypothesis that flashbacks can be psychologically determined symptoms is supported by the dynamics of the case and the course of treatment. A second focus is a partial explanation for the often made observation that obessive-compulsive personalities are at increased risk for LSD flashbacks.", "PMID": 978191}
{"id": "pubmed23n0032_11554", "title": "Characteristics of the sympathetic innervation of the nictitating membrane and of the vasculature of the nose and tongue of the cat.", "content": "Vasomotor responses from the nasal mucosa and tongue, and contractions of the nictitating membrane, were recorded on stimulation of the cervical sympathetic or internal carotid nerves. Preganglionic sympathetic nerve fibres which elicited a membrane response possessed a lower threshold than those which evoked nasal vasoconstriction, while the latter displayed a lower threshold than fibres which evoked tongue vasoconstriction. The sympathetic vasodilator fibres to the tongue whose activity was revealed after alpha-receptor blockade, had a similar threshold to the vasoconstrictor fibres. Membrane contraction, nasal vasoconstriction and occasionally tongue vasoconstriction could be evoked by stimulating the internal carotid nerve. The postganglionic fibres innervating the nasal mucosa had a similar threshold to those of the nictitating membrane, which may indicate that there are small myelinated fibres innervating the mucosa. The preganglionic compound nerve action potential had four major components, S1-S4. S1, S2 and usually S3 fibres were associated with membrane contraction; S2, S3 and sometimes S1 fibres were associated with nasal vasoconstriction; and S3, usually S2 and occasionally S1 fibres were associated with vasoconstriction in the tongue. It is concluded that each of these three groups of nerve fibres, but not S4 fibres, may include fibres associated functionally with the three effectors. There was a considerable difference between the relative amplitude of the responses of the three effectors elicited by stimulation of the cervical sympathetic nerve at frequencies between 0.2 and 2 Hz. Vasoconstrictor responses were relatively larger than membrane contractions suggesting differences in the mechanisms of neurotransmission at the neuroeffector junctions.", "contents": "Characteristics of the sympathetic innervation of the nictitating membrane and of the vasculature of the nose and tongue of the cat. Vasomotor responses from the nasal mucosa and tongue, and contractions of the nictitating membrane, were recorded on stimulation of the cervical sympathetic or internal carotid nerves. Preganglionic sympathetic nerve fibres which elicited a membrane response possessed a lower threshold than those which evoked nasal vasoconstriction, while the latter displayed a lower threshold than fibres which evoked tongue vasoconstriction. The sympathetic vasodilator fibres to the tongue whose activity was revealed after alpha-receptor blockade, had a similar threshold to the vasoconstrictor fibres. Membrane contraction, nasal vasoconstriction and occasionally tongue vasoconstriction could be evoked by stimulating the internal carotid nerve. The postganglionic fibres innervating the nasal mucosa had a similar threshold to those of the nictitating membrane, which may indicate that there are small myelinated fibres innervating the mucosa. The preganglionic compound nerve action potential had four major components, S1-S4. S1, S2 and usually S3 fibres were associated with membrane contraction; S2, S3 and sometimes S1 fibres were associated with nasal vasoconstriction; and S3, usually S2 and occasionally S1 fibres were associated with vasoconstriction in the tongue. It is concluded that each of these three groups of nerve fibres, but not S4 fibres, may include fibres associated functionally with the three effectors. There was a considerable difference between the relative amplitude of the responses of the three effectors elicited by stimulation of the cervical sympathetic nerve at frequencies between 0.2 and 2 Hz. Vasoconstrictor responses were relatively larger than membrane contractions suggesting differences in the mechanisms of neurotransmission at the neuroeffector junctions.", "PMID": 978192}
{"id": "pubmed23n0032_11555", "title": "[The embryonic pineal organ of Lacerta vivipara J. I. Differentiation of the cells of the sensory line (CSL). The gradient of morphological differentiation].", "content": "This paper deals with the cytological development of the cells of the sensory line (CSL) of the pineal organ in Lacerta vivipara J. from the embryonic stage 30 on to the third day after birth. The general results are diagrammatically represented in figure 29. Only from stage 35 on, a diversity of sensory line cells can be distinguished. During ontogenetic development, in the photoreceptor cells (P) secretory activity is not observed. From stage 38 on, the photoreceptive poles of these elements show a differentiation of disks while, from stage 37 on, their basal processes make synaptic contacts with dendritic outgrowths originating from intra-epithelial sensory nerve cells. The embryonic secretory rudimentary photoreceptor cells (SRP) are characterized by an intensive secretory activity. Our quantitative analysis reveals a highly significant increase, during ontogenetic development, of the number of dense secretory granules, stored in the basal processes of the SRP. The photoreceptive poles of the SRP, just like those of the P cells, show the differentiation of disks from stage 38 on. Only just after birth, the outer segments of the SRP become rudimentary. From the absence of characteristic synaptic contacts between the basal processes of the SRP and dendrites of the sensory nerve cells it is concluded that, during ontogenetic development, the SRP become rudimentary as photoreceptivity is concerned. During the ontogenetic development of the pineal organ in Lacerta vivipara, morphological signs pointing to regression of its photoreceptor function are not very marked. In showing a photoreceptive differentiation of the outer segments of the SRP, the present investigation agrees with phylogenetic studies by other authors and supports the concept of the presence of a sensory cell line in the vertebrate pineal organ (Collin, 1969 b).", "contents": "[The embryonic pineal organ of Lacerta vivipara J. I. Differentiation of the cells of the sensory line (CSL). The gradient of morphological differentiation]. This paper deals with the cytological development of the cells of the sensory line (CSL) of the pineal organ in Lacerta vivipara J. from the embryonic stage 30 on to the third day after birth. The general results are diagrammatically represented in figure 29. Only from stage 35 on, a diversity of sensory line cells can be distinguished. During ontogenetic development, in the photoreceptor cells (P) secretory activity is not observed. From stage 38 on, the photoreceptive poles of these elements show a differentiation of disks while, from stage 37 on, their basal processes make synaptic contacts with dendritic outgrowths originating from intra-epithelial sensory nerve cells. The embryonic secretory rudimentary photoreceptor cells (SRP) are characterized by an intensive secretory activity. Our quantitative analysis reveals a highly significant increase, during ontogenetic development, of the number of dense secretory granules, stored in the basal processes of the SRP. The photoreceptive poles of the SRP, just like those of the P cells, show the differentiation of disks from stage 38 on. Only just after birth, the outer segments of the SRP become rudimentary. From the absence of characteristic synaptic contacts between the basal processes of the SRP and dendrites of the sensory nerve cells it is concluded that, during ontogenetic development, the SRP become rudimentary as photoreceptivity is concerned. During the ontogenetic development of the pineal organ in Lacerta vivipara, morphological signs pointing to regression of its photoreceptor function are not very marked. In showing a photoreceptive differentiation of the outer segments of the SRP, the present investigation agrees with phylogenetic studies by other authors and supports the concept of the presence of a sensory cell line in the vertebrate pineal organ (Collin, 1969 b).", "PMID": 978193}
{"id": "pubmed23n0032_11556", "title": "Effect of the activation of alpha-adrenoreceptors on the synthesis and release of noradrenaline by peripheral adrenergic nerves in vivo.", "content": "The synthesis and release of noradrenaline (NA) in the heart and submaxillary glands were studied in the rat following s.c. injections of oxymetazoline (50 mug/kg) or noradrenaline (500 mug/kg). NA release was evaluated from the decline in tissular specific radioactivity after administration of 3H-NA and NA synthesis by the estimation of the amounts of 3H-NA synthesized from 3H-tyrosine (TY) or 3H-Dopa, 30 min after the injection. Oxymetazoline treatment delayed the release of NA, the NA biological half-lives rising from 12 up to 36 hours in the heart and from 5.9 up to 21 hours in sub-maxillary glands. This inhibitory effect on NA release was interpreted as the consequence of the stimulation of alpha-adrenoreceptors. Thirty minutes after its injection, oxymetazoline increased both NA endogenous levels and 3H-NA amounts formed from 3H-TY: 3H-NA specific activities were not significantly altered. NA treatment led to an acceleration of NA release in the heart (NA biological half-life decreasing from 12 to 2.2 hours) but not in sub-maxillary glands. After injection of 3H-TY, the amounts of 3H-NA found in the heart and sub-maxillary glands were strongly reduced. Similar results were observed in the heart using 3H-Dopa as a precursor. These data are interpreted as the consequence of the removal of the newly synthesized 3H-NA by exogenous NA. The results obtained with oxymetazoline point out a dissociation between the NA release which is reduced and the NA synthesis which is unaltered. This indicates that NA synthesis rate by sympathetic nerve terminals is not immediately regulated by its release intensity. These data do not support the end-product feedback inhibition hypothesis according to which tyrosine hydroxylase is regulated by the intraneuronal NA concentration.", "contents": "Effect of the activation of alpha-adrenoreceptors on the synthesis and release of noradrenaline by peripheral adrenergic nerves in vivo. The synthesis and release of noradrenaline (NA) in the heart and submaxillary glands were studied in the rat following s.c. injections of oxymetazoline (50 mug/kg) or noradrenaline (500 mug/kg). NA release was evaluated from the decline in tissular specific radioactivity after administration of 3H-NA and NA synthesis by the estimation of the amounts of 3H-NA synthesized from 3H-tyrosine (TY) or 3H-Dopa, 30 min after the injection. Oxymetazoline treatment delayed the release of NA, the NA biological half-lives rising from 12 up to 36 hours in the heart and from 5.9 up to 21 hours in sub-maxillary glands. This inhibitory effect on NA release was interpreted as the consequence of the stimulation of alpha-adrenoreceptors. Thirty minutes after its injection, oxymetazoline increased both NA endogenous levels and 3H-NA amounts formed from 3H-TY: 3H-NA specific activities were not significantly altered. NA treatment led to an acceleration of NA release in the heart (NA biological half-life decreasing from 12 to 2.2 hours) but not in sub-maxillary glands. After injection of 3H-TY, the amounts of 3H-NA found in the heart and sub-maxillary glands were strongly reduced. Similar results were observed in the heart using 3H-Dopa as a precursor. These data are interpreted as the consequence of the removal of the newly synthesized 3H-NA by exogenous NA. The results obtained with oxymetazoline point out a dissociation between the NA release which is reduced and the NA synthesis which is unaltered. This indicates that NA synthesis rate by sympathetic nerve terminals is not immediately regulated by its release intensity. These data do not support the end-product feedback inhibition hypothesis according to which tyrosine hydroxylase is regulated by the intraneuronal NA concentration.", "PMID": 978194}
{"id": "pubmed23n0032_11557", "title": "Evidence for involvement of central noradrenergic neurons in the cardiovascular depression induced by morphine in the rat.", "content": "Morphine caused in the anaesthetized rat reduction in brain noradrenaline (NA) turnover, hypotension and bradycardia, similarly to the antihypertensive, alpha-adrenergic agonist, clonidine. All effects of morphine were antagonized by naloxone, as well as the alpha-receptor antagonist, yohimbine. In contrast, naloxone did not affect the circulatory depression and reduction in brain NA utilization by clonidine which both previously have been found to be antagonized by yohimbine. In contrast to clonidine, morphine even in high doses did not facilitate the flexor reflex activity of acutely spinalized rats. Pretreatment with protriptylin largely attenuated the circulatory depressive effects of morphine, as it has previously been found to block the corresponding effects of clonidine. Thus, the morphine-induced cardiovascular depressive effects are primarily elicited by activation of opiate receptors. However, the inhibition of brain NA neurotransmission by morphine appears critically involved in the mediation of the circulatory depression.", "contents": "Evidence for involvement of central noradrenergic neurons in the cardiovascular depression induced by morphine in the rat. Morphine caused in the anaesthetized rat reduction in brain noradrenaline (NA) turnover, hypotension and bradycardia, similarly to the antihypertensive, alpha-adrenergic agonist, clonidine. All effects of morphine were antagonized by naloxone, as well as the alpha-receptor antagonist, yohimbine. In contrast, naloxone did not affect the circulatory depression and reduction in brain NA utilization by clonidine which both previously have been found to be antagonized by yohimbine. In contrast to clonidine, morphine even in high doses did not facilitate the flexor reflex activity of acutely spinalized rats. Pretreatment with protriptylin largely attenuated the circulatory depressive effects of morphine, as it has previously been found to block the corresponding effects of clonidine. Thus, the morphine-induced cardiovascular depressive effects are primarily elicited by activation of opiate receptors. However, the inhibition of brain NA neurotransmission by morphine appears critically involved in the mediation of the circulatory depression.", "PMID": 978195}
{"id": "pubmed23n0032_11558", "title": "Distribution of monoamine oxidase (MAO) in relation to the hypothalamo-hypophyseal system of the teleost Clarias batrachus (L.).", "content": "Varying degrees of monoamine oxidase (MAO) activity were noticed all along the hypothalamo-hypophyseal neurosecretory tract in C. batrachus. Intense activity was evident on the wall of the secretory cells of the adenohypophysis, neurons of the nucleus preopticus (NPO) and nucleus lateralis tuberis (NLT). However, MAO activity was also observed in the cytoplasm of some neurons in the ventromedian region of the NLT and groups of cells present in the neurohypophyseal ramifications of the pars intermedia. A strong accumulation of formazan granules in the median eminence (ME) and the neuroadeno interface lends further support to the view that C. batrachus has a ME of both the tetrapodan and the teleostean type. The distributional pattern of MAO in the hypothalamus and hypophysis suggests that monoaminergic fibres are probably involved in the secretory mechanism of the releasing factors and pituitary hormones.", "contents": "Distribution of monoamine oxidase (MAO) in relation to the hypothalamo-hypophyseal system of the teleost Clarias batrachus (L.). Varying degrees of monoamine oxidase (MAO) activity were noticed all along the hypothalamo-hypophyseal neurosecretory tract in C. batrachus. Intense activity was evident on the wall of the secretory cells of the adenohypophysis, neurons of the nucleus preopticus (NPO) and nucleus lateralis tuberis (NLT). However, MAO activity was also observed in the cytoplasm of some neurons in the ventromedian region of the NLT and groups of cells present in the neurohypophyseal ramifications of the pars intermedia. A strong accumulation of formazan granules in the median eminence (ME) and the neuroadeno interface lends further support to the view that C. batrachus has a ME of both the tetrapodan and the teleostean type. The distributional pattern of MAO in the hypothalamus and hypophysis suggests that monoaminergic fibres are probably involved in the secretory mechanism of the releasing factors and pituitary hormones.", "PMID": 978196}
{"id": "pubmed23n0032_11559", "title": "Molecular biology of neurological and psychiatric disorders. I. Effect of parkinsonism, age, sex and L-dopa on platelet monoamine oxidase.", "content": "Since there is substantial evidence for a nigrostriatal dopamine defect in Parkinson's disease and since monoamine oxidase (MAO) appears to be essential for the degradation of dopamine, we investigated whether this enzyme is involved in the pathogenesis of this disease or in the therapeutic action of L-dopa. To gain a solid basis for our analysis we studied some properties of platelet MAO, at present the only practical in vivo source for human MAO. Substrate and inhibitor pattern clearly pointed to a predominant B-type character of this enzyme. By using 3 substrates, m-iodobenzylamine, p-methoxybenzylamine, and tyramine, we found a marked age and sex difference in MAO activity. In untreated parkinsonian patients, platelet MAO was slightly reduced as compared with age- and sex-matched controls. Treatment with L-dopa induced a further reduction of platelet MAO activity in both sexes, but more in men than in women. We conjecture that the action of L-dopa on parkisonian patients is twofold: L-dopa is known to enhance the release of gonadotropins and thus to increase the production of sex hormones which in turn are capable of reducing MAO activity. Dopamine, formed from L-dopa, may thus have a better chance for survival in reaching the dopaminergic receptor. A new form of therapy, based on this concept, is proposed.", "contents": "Molecular biology of neurological and psychiatric disorders. I. Effect of parkinsonism, age, sex and L-dopa on platelet monoamine oxidase. Since there is substantial evidence for a nigrostriatal dopamine defect in Parkinson's disease and since monoamine oxidase (MAO) appears to be essential for the degradation of dopamine, we investigated whether this enzyme is involved in the pathogenesis of this disease or in the therapeutic action of L-dopa. To gain a solid basis for our analysis we studied some properties of platelet MAO, at present the only practical in vivo source for human MAO. Substrate and inhibitor pattern clearly pointed to a predominant B-type character of this enzyme. By using 3 substrates, m-iodobenzylamine, p-methoxybenzylamine, and tyramine, we found a marked age and sex difference in MAO activity. In untreated parkinsonian patients, platelet MAO was slightly reduced as compared with age- and sex-matched controls. Treatment with L-dopa induced a further reduction of platelet MAO activity in both sexes, but more in men than in women. We conjecture that the action of L-dopa on parkisonian patients is twofold: L-dopa is known to enhance the release of gonadotropins and thus to increase the production of sex hormones which in turn are capable of reducing MAO activity. Dopamine, formed from L-dopa, may thus have a better chance for survival in reaching the dopaminergic receptor. A new form of therapy, based on this concept, is proposed.", "PMID": 978197}
{"id": "pubmed23n0032_11560", "title": "Acute effects of histamine on plasma prolactin and luteininzing hormone levels in male rats.", "content": "Histamine injected into the 3rd ventricle of normal male rats at doses of 5-60 mug (free base) caused a marked release of prolactin. Responses were prevented by the antihistamine chlorpheniramine but not by atropine, methysergide or phenoxybenzamine. It thus seems that effects of histamine on prolactin are specific and not mediated by other neurotransmitters. Plasma LH remained normal after injection of low doses but it was decreased after high doses. The results obtained indicate a facilitatory role of histamine on prolactin release.", "contents": "Acute effects of histamine on plasma prolactin and luteininzing hormone levels in male rats. Histamine injected into the 3rd ventricle of normal male rats at doses of 5-60 mug (free base) caused a marked release of prolactin. Responses were prevented by the antihistamine chlorpheniramine but not by atropine, methysergide or phenoxybenzamine. It thus seems that effects of histamine on prolactin are specific and not mediated by other neurotransmitters. Plasma LH remained normal after injection of low doses but it was decreased after high doses. The results obtained indicate a facilitatory role of histamine on prolactin release.", "PMID": 978198}
{"id": "pubmed23n0032_11561", "title": "Pharmacological evidence for a selective antidopaminergic action of gamma-hydroxybutyric acid.", "content": "D-Amphetamine (Amph) and p-chloroamphetamine (PCA) induced dose-dependent increases in oropharyngeal myocloniform twitch activity (MTA) in rats anesthetized with urethane. In doses of 80-120 mg/kg, gamma-hydroxybutyric acid (GHB) blocked Amph-induced MTA. The blockade was readily surmountable. Pretreatment with reserpine markedly enhanced the myoclonigenic effect of Amph and rendered it insensitive blockade by GHB, 160 mg/kg. PCA and tryptamine also effectively stimulated MTA, but unlike Amph were antagonized by low doses of the serotonin (5-HT) antagonist methysergide. In doses which blocked Amph, GHB failed to antagonize the myoclonigenic effect of PCA. It is concluded that: (a) the actions of Amph and PCA on MTA is less sensitive to GHB blockade than DA-mediated MTA; and (c) the GHB-Amph antagonism may be of a functional nature, i.e. result from a depression of the firing activity of DA neurons produced by GHB. Since reserpinization abolished the GHB effect on Amph-induced MTA, the functional integrity of granular DA binding and releasing mechanisms appears to be a pre-requisite for the antagonism between GHB and Amph.", "contents": "Pharmacological evidence for a selective antidopaminergic action of gamma-hydroxybutyric acid. D-Amphetamine (Amph) and p-chloroamphetamine (PCA) induced dose-dependent increases in oropharyngeal myocloniform twitch activity (MTA) in rats anesthetized with urethane. In doses of 80-120 mg/kg, gamma-hydroxybutyric acid (GHB) blocked Amph-induced MTA. The blockade was readily surmountable. Pretreatment with reserpine markedly enhanced the myoclonigenic effect of Amph and rendered it insensitive blockade by GHB, 160 mg/kg. PCA and tryptamine also effectively stimulated MTA, but unlike Amph were antagonized by low doses of the serotonin (5-HT) antagonist methysergide. In doses which blocked Amph, GHB failed to antagonize the myoclonigenic effect of PCA. It is concluded that: (a) the actions of Amph and PCA on MTA is less sensitive to GHB blockade than DA-mediated MTA; and (c) the GHB-Amph antagonism may be of a functional nature, i.e. result from a depression of the firing activity of DA neurons produced by GHB. Since reserpinization abolished the GHB effect on Amph-induced MTA, the functional integrity of granular DA binding and releasing mechanisms appears to be a pre-requisite for the antagonism between GHB and Amph.", "PMID": 978199}
{"id": "pubmed23n0032_11562", "title": "A histochemical investigation of catecholamines in spinal cord injury.", "content": "The catecholamine hypothesis of progressive spinal cord necrosis following mechanical trauma was investigated with the histofluorometric method. Forty-four adult mongrel dog were examined as control, L1 crush-injured, and crush-injured with prior T1 total transection groups. In crush injured dogs, catecholamines were present in a 1 cm length of white matter at the crush site, with the greatest accumulation in the deep lateral and ventral funiculi. Gray matter fluorescence was not enhanced. Prior transection did not abolish the intense accumulation of catecholamines at the site of the cord injury. We propose that the catecholamines accumulating at the cord injured site are not central in origin, but represent an uptake mechanism into white matter as a reflection of cord microperfusion.", "contents": "A histochemical investigation of catecholamines in spinal cord injury. The catecholamine hypothesis of progressive spinal cord necrosis following mechanical trauma was investigated with the histofluorometric method. Forty-four adult mongrel dog were examined as control, L1 crush-injured, and crush-injured with prior T1 total transection groups. In crush injured dogs, catecholamines were present in a 1 cm length of white matter at the crush site, with the greatest accumulation in the deep lateral and ventral funiculi. Gray matter fluorescence was not enhanced. Prior transection did not abolish the intense accumulation of catecholamines at the site of the cord injury. We propose that the catecholamines accumulating at the cord injured site are not central in origin, but represent an uptake mechanism into white matter as a reflection of cord microperfusion.", "PMID": 978200}
{"id": "pubmed23n0032_11563", "title": "Effect of pinealectomy on gonadotrophins in immature female rats.", "content": "Pinealectomy, performed in new-born female rats, causes a temporary decrease in pituitary LH content between the 21st and the 30th day of age as compared with the controls. In sham-operated rats a smaller decrease is observed.", "contents": "Effect of pinealectomy on gonadotrophins in immature female rats. Pinealectomy, performed in new-born female rats, causes a temporary decrease in pituitary LH content between the 21st and the 30th day of age as compared with the controls. In sham-operated rats a smaller decrease is observed.", "PMID": 978201}
{"id": "pubmed23n0032_11564", "title": "Alteration of protein metabolism in individual, identified neurons from Aplysia.", "content": "A search for control mechanisms governing protein metabolism in neurons from Aplysia californica has uncovered two examples of altered patterns of newly synthesized proteins: (1) The pattern of newly synthesized proteins in the R2 neuron is altered when protein synthesis occurs at elevated temperatures (22-30 degrees C as compared with 13-15 degrees C). (2) The processing of newly synthesized 12,000 dalton (12k) material to 6-9,000 dalton (6-9k) size in the R15 neuron (Strumwasser, F. and Wilson, D.F. [1976], J. Gen. Physiol., in press) can be blocked by certain ion replacements. If acetate replaces chloride in the incubation medium during the synthesis of 12k material, an early step in the processing, prior to the actual breakdown of 12k material, is blocked. Experiments with RNA-synthesis inhibitors indicate that none of the mRNAs which code for abundantly synthesized protein species in the R2 or R15 neurons have short (less than 4 hr) half-lives. This result has implications for an earlier report of regulation of protein synthesis in the R15 neuron.", "contents": "Alteration of protein metabolism in individual, identified neurons from Aplysia. A search for control mechanisms governing protein metabolism in neurons from Aplysia californica has uncovered two examples of altered patterns of newly synthesized proteins: (1) The pattern of newly synthesized proteins in the R2 neuron is altered when protein synthesis occurs at elevated temperatures (22-30 degrees C as compared with 13-15 degrees C). (2) The processing of newly synthesized 12,000 dalton (12k) material to 6-9,000 dalton (6-9k) size in the R15 neuron (Strumwasser, F. and Wilson, D.F. [1976], J. Gen. Physiol., in press) can be blocked by certain ion replacements. If acetate replaces chloride in the incubation medium during the synthesis of 12k material, an early step in the processing, prior to the actual breakdown of 12k material, is blocked. Experiments with RNA-synthesis inhibitors indicate that none of the mRNAs which code for abundantly synthesized protein species in the R2 or R15 neurons have short (less than 4 hr) half-lives. This result has implications for an earlier report of regulation of protein synthesis in the R15 neuron.", "PMID": 978202}
{"id": "pubmed23n0032_11565", "title": "Effects of different ions on resting polarization and on the mass receptor potential of carotid body chemosensors.", "content": "The carotid body and its own nerve were removed from cats anesthetized with sodium pentobarbital and placed in an air gap system; the carotid body was bathed in modified Locke's solution equilibrated with 50% O2 in N2, pH 7.43 at 35 degrees C. The sensory discharges, changes in \"resting\" receptor polarization and the mass receptor potential evoked by ACh or NaCN were recorded with nonpolarizable electrodes placed across the gap. Receptor potentials and sensory discharges evoked by ACh showed an appreciable increase in amplitude and frequency when the preparation was bathed in eserinized Locke. Eserine did not change appreciably the responses evoked by NaCN. Excessive depolarization elicited by either ACh or NaCN was accompanied by sensory discharge block. Removal of K+ ions from the bathing solution induced receptor hyperpolarization and an increase in the amplitude of the evoked receptor potentials. An increase of K+ concentration had the opposite effect. Reduction of Na+ or NaCl to one half, or total removal of this salt, induced an initial reduction and later disappearance of the sensory discharges, some receptor hyperpolarization and a reduction in the amplitude of the evoked receptor potential. Reduction or removal of Ca++ produced receptor depolarization, a marked depression of the evoked receptor potentials, an increase in the frequency of the sensory discharges and a reduction in the amplitude of the nerve action potentials. High Ca++ or Mg++ had little or no effect on action potential amplitude or resting polarization, but decreased sensory discharge frequency and the evoked receptor potentials. Total or partial replacement of Ca++ with Mg++ induced complex effects: (1) receptor depolarization which occurred in low Ca++, was prevented by addition of Mg++ ions; (2) the amplitude of the evoked receptor potentials was depressed; (3) the nerve discharge frequency was reduced as it was in high Mg++ solutions; and (4) the amplitude of the nerve action potentials was reduced as it was in low Ca++ solutions. Temperature had a marked effect on the chemoreceptors since at high temperatures the receptors were depolarized and the discharge frequency increased. The baseline discharge and responses evoked by ACh or NaCN were depressed at low temperatures. The results are discussed in terms of possible receptor mechanisms influenced by the different ions.", "contents": "Effects of different ions on resting polarization and on the mass receptor potential of carotid body chemosensors. The carotid body and its own nerve were removed from cats anesthetized with sodium pentobarbital and placed in an air gap system; the carotid body was bathed in modified Locke's solution equilibrated with 50% O2 in N2, pH 7.43 at 35 degrees C. The sensory discharges, changes in \"resting\" receptor polarization and the mass receptor potential evoked by ACh or NaCN were recorded with nonpolarizable electrodes placed across the gap. Receptor potentials and sensory discharges evoked by ACh showed an appreciable increase in amplitude and frequency when the preparation was bathed in eserinized Locke. Eserine did not change appreciably the responses evoked by NaCN. Excessive depolarization elicited by either ACh or NaCN was accompanied by sensory discharge block. Removal of K+ ions from the bathing solution induced receptor hyperpolarization and an increase in the amplitude of the evoked receptor potentials. An increase of K+ concentration had the opposite effect. Reduction of Na+ or NaCl to one half, or total removal of this salt, induced an initial reduction and later disappearance of the sensory discharges, some receptor hyperpolarization and a reduction in the amplitude of the evoked receptor potential. Reduction or removal of Ca++ produced receptor depolarization, a marked depression of the evoked receptor potentials, an increase in the frequency of the sensory discharges and a reduction in the amplitude of the nerve action potentials. High Ca++ or Mg++ had little or no effect on action potential amplitude or resting polarization, but decreased sensory discharge frequency and the evoked receptor potentials. Total or partial replacement of Ca++ with Mg++ induced complex effects: (1) receptor depolarization which occurred in low Ca++, was prevented by addition of Mg++ ions; (2) the amplitude of the evoked receptor potentials was depressed; (3) the nerve discharge frequency was reduced as it was in high Mg++ solutions; and (4) the amplitude of the nerve action potentials was reduced as it was in low Ca++ solutions. Temperature had a marked effect on the chemoreceptors since at high temperatures the receptors were depolarized and the discharge frequency increased. The baseline discharge and responses evoked by ACh or NaCN were depressed at low temperatures. The results are discussed in terms of possible receptor mechanisms influenced by the different ions.", "PMID": 978203}
{"id": "pubmed23n0032_11566", "title": "Creatine kinase isoenzymes in neuromuscular diseases.", "content": "Determination of the creatine kinase isoenzyme pattern in 62 biopsy samples obtained from patients with neuromuscular disease revealed changes mainly in Duchenne muscular dystrophy. The BB isoenzyme was detected in 10 out of 17 cases with Duchenne muscular dystrophy and the relative amount of MB+BB isoenzyme was significantly increased in this group (P less than 0.005). In serum the MB isoenzyme was detected in all 28 cases with progressive muscular dystrophy and frequently also in other neuromuscular diseases. Among 152 control samples the MB isoenzyme was detected only in 2 cases. It is suggested that the finding of MB isoenzyme in the serum with normal or only slightly elevated total CK activity may be a further proof of neuromuscular disorder, but the finding is not specific for any particular disease.", "contents": "Creatine kinase isoenzymes in neuromuscular diseases. Determination of the creatine kinase isoenzyme pattern in 62 biopsy samples obtained from patients with neuromuscular disease revealed changes mainly in Duchenne muscular dystrophy. The BB isoenzyme was detected in 10 out of 17 cases with Duchenne muscular dystrophy and the relative amount of MB+BB isoenzyme was significantly increased in this group (P less than 0.005). In serum the MB isoenzyme was detected in all 28 cases with progressive muscular dystrophy and frequently also in other neuromuscular diseases. Among 152 control samples the MB isoenzyme was detected only in 2 cases. It is suggested that the finding of MB isoenzyme in the serum with normal or only slightly elevated total CK activity may be a further proof of neuromuscular disorder, but the finding is not specific for any particular disease.", "PMID": 978205}
{"id": "pubmed23n0032_11567", "title": "Some quantitative morphological aspects of post-natal human cerebellar growth.", "content": "Using a Quantimet 720 image analysing computer, the cellularity of the external and internal granular layer and the molecular layer has been measured in standard blocks of the lateral and central lobes of the cerebellum of apparently normal children. The DNA content of the nerve cells has also been measured and related to post-natal age. Standard cell concentrations per unit area have been established from birth to 14 years. The different lobules of the central lobe show different patterns of cellular development but all lobules reach their fully mature cellularity by 2 years. The maximum cellularity of the internal granular layer is maintained for several years, after which there is an apparent progressive reduction in the concentration of cells per unit area. Alterations in the DNA staining properties of the cell nuclei of the internal granular layer during normal development are presented.", "contents": "Some quantitative morphological aspects of post-natal human cerebellar growth. Using a Quantimet 720 image analysing computer, the cellularity of the external and internal granular layer and the molecular layer has been measured in standard blocks of the lateral and central lobes of the cerebellum of apparently normal children. The DNA content of the nerve cells has also been measured and related to post-natal age. Standard cell concentrations per unit area have been established from birth to 14 years. The different lobules of the central lobe show different patterns of cellular development but all lobules reach their fully mature cellularity by 2 years. The maximum cellularity of the internal granular layer is maintained for several years, after which there is an apparent progressive reduction in the concentration of cells per unit area. Alterations in the DNA staining properties of the cell nuclei of the internal granular layer during normal development are presented.", "PMID": 978206}
{"id": "pubmed23n0032_11568", "title": "Erythrocyte spectrin peak II phosphorylation in Duchenne muscular dystrophy.", "content": "Duchenne muscular dystrophy (DMD) is a rapidly progressive crippling disease of young boys that is inherited as an X-linked recessive trait. Previous studies have demonstrated the usefulness of erythrocyte studies in exploring membrane abnormalities in inheritied muscular dystrophy. Erythrocyte spectrin peak II protein (m.w. equivalent to 220,000) was more highly phosphorylated under initial rate conditions in DMD than in controls. The extent of peak II phosphorylation was greater in DMD erythrocytes and a Na+ stimulated peak II phosphorylation effect (Avruch and Fairbanks 1974) was not found to account for the differences between DMD and controls. The phosphorylated state of spectrin proteins in the membrane was evaluated and no differences in DMD could be measured. The maximal transfer of phosphate from differences in DMD could be measured. The maximal transfer of phosphate from [gamma-32P]ATP to spectrin peak II accounts for approximately 5-10% of the total phosphate content of spectrin.", "contents": "Erythrocyte spectrin peak II phosphorylation in Duchenne muscular dystrophy. Duchenne muscular dystrophy (DMD) is a rapidly progressive crippling disease of young boys that is inherited as an X-linked recessive trait. Previous studies have demonstrated the usefulness of erythrocyte studies in exploring membrane abnormalities in inheritied muscular dystrophy. Erythrocyte spectrin peak II protein (m.w. equivalent to 220,000) was more highly phosphorylated under initial rate conditions in DMD than in controls. The extent of peak II phosphorylation was greater in DMD erythrocytes and a Na+ stimulated peak II phosphorylation effect (Avruch and Fairbanks 1974) was not found to account for the differences between DMD and controls. The phosphorylated state of spectrin proteins in the membrane was evaluated and no differences in DMD could be measured. The maximal transfer of phosphate from differences in DMD could be measured. The maximal transfer of phosphate from [gamma-32P]ATP to spectrin peak II accounts for approximately 5-10% of the total phosphate content of spectrin.", "PMID": 978207}
{"id": "pubmed23n0032_11569", "title": "Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications.", "content": "Polyacrylamide-gel disc electrophoresis has been applied to the fractionation of CSF samples obtained from 2m control subjects (range of age 16 to 77 yr, mean 41.0 yr) and from 157 patients with various neurological diseases, with the purpose of evaluating the diagnostic significance of separated protein bands. It was found that the immunoglobulins, with some exceptions, were fractionated in the G-zone which was defined as an area between the origin and 0.57 in terms of relative migration based on transferrin (RT). Fourty-four percent of the whole Bence Jones protein bands which were examined were detected in the area of RT 0.70-0.90. The normal values of CSF protein fractions were (means+/-SD):Pre-alb-zone, 10.97+/-2.31%; Alb-zone, 40.74+/-5.72%; A1-zone, 5.25+/-0.72%; A2-zone, 8.23+/-1.24%; B1-zone, 10.82+/-2.18%. B2-zone, 7.94+/-1.93%; G-zone, 16.05+/-2.49%. The following significant results were obtained: (1) When the total protein concentration (TP) of CSF is increased: the percentage of the Pre-alb-zone is lowered and that of the A1- and G-zones is increased in diseases such as neurosyphilis, infectious meningitis, brain tumour, multiple sclerosis, intervertebral disc protrusion and cerebral infarction. The A2-zone was diminished in cerebral infarction, and a similar change was seen in brain tumour with in addition a decrease of the B2-zone; (2) When the TP was normal: the percentage of the G-zone was increased in multiple sclerosis, brain tumour and intervertebral disc protrusion. In the latter 2 disorders the A1-zone also increased. This fraction also increased in the cases of cerebral infarction, whose mean age was, however, much higher than that of the control subjects.", "contents": "Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications. Polyacrylamide-gel disc electrophoresis has been applied to the fractionation of CSF samples obtained from 2m control subjects (range of age 16 to 77 yr, mean 41.0 yr) and from 157 patients with various neurological diseases, with the purpose of evaluating the diagnostic significance of separated protein bands. It was found that the immunoglobulins, with some exceptions, were fractionated in the G-zone which was defined as an area between the origin and 0.57 in terms of relative migration based on transferrin (RT). Fourty-four percent of the whole Bence Jones protein bands which were examined were detected in the area of RT 0.70-0.90. The normal values of CSF protein fractions were (means+/-SD):Pre-alb-zone, 10.97+/-2.31%; Alb-zone, 40.74+/-5.72%; A1-zone, 5.25+/-0.72%; A2-zone, 8.23+/-1.24%; B1-zone, 10.82+/-2.18%. B2-zone, 7.94+/-1.93%; G-zone, 16.05+/-2.49%. The following significant results were obtained: (1) When the total protein concentration (TP) of CSF is increased: the percentage of the Pre-alb-zone is lowered and that of the A1- and G-zones is increased in diseases such as neurosyphilis, infectious meningitis, brain tumour, multiple sclerosis, intervertebral disc protrusion and cerebral infarction. The A2-zone was diminished in cerebral infarction, and a similar change was seen in brain tumour with in addition a decrease of the B2-zone; (2) When the TP was normal: the percentage of the G-zone was increased in multiple sclerosis, brain tumour and intervertebral disc protrusion. In the latter 2 disorders the A1-zone also increased. This fraction also increased in the cases of cerebral infarction, whose mean age was, however, much higher than that of the control subjects.", "PMID": 978208}
{"id": "pubmed23n0032_11570", "title": "Myofibrillar protein pattern in experimental myotonia in rats.", "content": "Experimental myotonia was induced in rats by long-term administration of 20,25-diazacholesterol. Electrophysiological, morphological and biochemical investigations were carried out on m. soleus, m. extensor digitorum longus and m. gastrocnemius. The effect of 20,25-diazacholesterol administration on myofibrillar proteins was studied and a significant rise in the concentration of a protein presumed to be alpha-actinin was demonstrated in m. gastrocnemius. A change of the same character, not statistically significant, was observed in the m. extensor digitorum longus.", "contents": "Myofibrillar protein pattern in experimental myotonia in rats. Experimental myotonia was induced in rats by long-term administration of 20,25-diazacholesterol. Electrophysiological, morphological and biochemical investigations were carried out on m. soleus, m. extensor digitorum longus and m. gastrocnemius. The effect of 20,25-diazacholesterol administration on myofibrillar proteins was studied and a significant rise in the concentration of a protein presumed to be alpha-actinin was demonstrated in m. gastrocnemius. A change of the same character, not statistically significant, was observed in the m. extensor digitorum longus.", "PMID": 978209}
{"id": "pubmed23n0032_11571", "title": "Abnormal tissue distribution of lead in amyotrophic lateral sclerosis.", "content": "The lead content of cerebrospinal fluid (CSF) was found to be significantly elevated in 12 patients with amyotrophic lateral sclerosis, when compared to 28 control subjects having non-degenerative neurological disorders. The difference could not be explained as being merely secondary to blood-CSF barrier damage. A hypothetical model of the pathogenesis of the disease is advanced and the results are discussed in relation to this model.", "contents": "Abnormal tissue distribution of lead in amyotrophic lateral sclerosis. The lead content of cerebrospinal fluid (CSF) was found to be significantly elevated in 12 patients with amyotrophic lateral sclerosis, when compared to 28 control subjects having non-degenerative neurological disorders. The difference could not be explained as being merely secondary to blood-CSF barrier damage. A hypothetical model of the pathogenesis of the disease is advanced and the results are discussed in relation to this model.", "PMID": 978210}
{"id": "pubmed23n0032_11572", "title": "Reactive hyperglycaemia in patients with acute stroke.", "content": "Initial and follow-up fasting serum glucose levels following acute stroke were evaluated retrospectively in 392 selected hospitalized patients. Transitory reactive hyperglycaemia was observed in a large number of patients (28% of the total series) without a history of diabetes prior to the acute cerebrovascular event. The data from this group suggest a possible relationship between the impairment of carbohydrate metabolism and the type and location of stroke since both the frequency and severity of the hyperglycaemic response were higher in patients with haemorrhagic stroke and brainstem infarction as compared with cerebral infarction. The incidence and degree of the reactive hyperglycaemia were also related to the severity of the acute stroke. There were more comatose patients in the group showing this phenomenon. Initial serum glucose levels in the latter group were higher in unconscious patients than in alert ones. In addition, hospital mortality was significantly higher in these patients. Transitory reactive increases of serum glucose levels were also observed in the majority of patients with a history of overt diabetes prior to the acute stroke. The hyperglycaemic reaction following acute stroke may be attributed to several underlying mechanisms. These include: a non-specific reaction to acute stress and tissue injury with the associated autonomic, hormonal and metabolic alterations; uncovering of underlying latent diabetes by the acute stroke; increased secretion of growth hormone due to stroke-induced hypothalamic dysfunction; and irritation of the glucose regulatory centres in the hypothalamus and brain stem by blood-laden cerebrospinal fluid or local ischaemia.", "contents": "Reactive hyperglycaemia in patients with acute stroke. Initial and follow-up fasting serum glucose levels following acute stroke were evaluated retrospectively in 392 selected hospitalized patients. Transitory reactive hyperglycaemia was observed in a large number of patients (28% of the total series) without a history of diabetes prior to the acute cerebrovascular event. The data from this group suggest a possible relationship between the impairment of carbohydrate metabolism and the type and location of stroke since both the frequency and severity of the hyperglycaemic response were higher in patients with haemorrhagic stroke and brainstem infarction as compared with cerebral infarction. The incidence and degree of the reactive hyperglycaemia were also related to the severity of the acute stroke. There were more comatose patients in the group showing this phenomenon. Initial serum glucose levels in the latter group were higher in unconscious patients than in alert ones. In addition, hospital mortality was significantly higher in these patients. Transitory reactive increases of serum glucose levels were also observed in the majority of patients with a history of overt diabetes prior to the acute stroke. The hyperglycaemic reaction following acute stroke may be attributed to several underlying mechanisms. These include: a non-specific reaction to acute stress and tissue injury with the associated autonomic, hormonal and metabolic alterations; uncovering of underlying latent diabetes by the acute stroke; increased secretion of growth hormone due to stroke-induced hypothalamic dysfunction; and irritation of the glucose regulatory centres in the hypothalamus and brain stem by blood-laden cerebrospinal fluid or local ischaemia.", "PMID": 978211}
{"id": "pubmed23n0032_11573", "title": "A trial of antilymphocyte globulin in the treatment of chronic progressive multiple sclerosis.", "content": "Twenty-three patients with chronic progressive multiple sclerosis (MS), resistant to steroid therapy, were treated with antilymphocyte globulin (ALG) and prednisone (30 mg by mouth daily). Twelve patients completed a full course of therapy (2 ml ALG i.m. daily for 2 weeks and 2 ml i.m. on alternate days for 2 weeks) and 6 others completed at least 2 weeks of daily injections. Six patients experienced an overall improvement of at least 15% using a comprehensive neurological scoring system. Three other patients had limited, but functionally useful improvement in specific neurologic functions. Six months after the completion of therapy, no patient had deteriorated to a level of function below that noted prior to treatment. Adverse reactions, which often necessitated stopping treatment, included fever, local inflammatory reactions, local rash, general malaise, mild anaphylactoid reactions, and enlargement and tenderness of regional lymph nodes. Because of the short duration of immunosuppression and the toxic side effects of ALG, we do not feel that ALG treatment yet deserves to be intorduced as a standard treatment in clinical practice. However, the improvement or arrest of progression seen in these patients who were deteriorating progressively despite steroid therapy would seem to justify a continued search for a safer method of suppressing immunity in MS patients.", "contents": "A trial of antilymphocyte globulin in the treatment of chronic progressive multiple sclerosis. Twenty-three patients with chronic progressive multiple sclerosis (MS), resistant to steroid therapy, were treated with antilymphocyte globulin (ALG) and prednisone (30 mg by mouth daily). Twelve patients completed a full course of therapy (2 ml ALG i.m. daily for 2 weeks and 2 ml i.m. on alternate days for 2 weeks) and 6 others completed at least 2 weeks of daily injections. Six patients experienced an overall improvement of at least 15% using a comprehensive neurological scoring system. Three other patients had limited, but functionally useful improvement in specific neurologic functions. Six months after the completion of therapy, no patient had deteriorated to a level of function below that noted prior to treatment. Adverse reactions, which often necessitated stopping treatment, included fever, local inflammatory reactions, local rash, general malaise, mild anaphylactoid reactions, and enlargement and tenderness of regional lymph nodes. Because of the short duration of immunosuppression and the toxic side effects of ALG, we do not feel that ALG treatment yet deserves to be intorduced as a standard treatment in clinical practice. However, the improvement or arrest of progression seen in these patients who were deteriorating progressively despite steroid therapy would seem to justify a continued search for a safer method of suppressing immunity in MS patients.", "PMID": 978212}
{"id": "pubmed23n0032_11574", "title": "Myelin composition in acute and chronic multiple sclerosis in relation to cerebral lysosomal activity.", "content": "The neuropathology of 3 cases of acute multiple sclerosis was correlated with biochemical analyses. Astrocytosis was a characteristic feature of the diffuse demyelinating lesions in one case and lymphocytic cuffing characterized the well-defined plaques present in the white matter of the other two cases. No abnormalities were found in the protein or lipid composition of isolated myelin, despite a wide range of recovery. Nevertheless, the gel electrophoretic protein pattern of white matter adjacent to plaque areas showed selective loss of myelin basic protein. Lysosomal acid proteinase and beta-glucuronidase levels were very significantly increased in all white matter samples in which astrocytosis was a major neuropathological feature. Levels were less markedly raised in samples containing discrete active plaques. Enzyme changes were also found in the apparently normal white matter of 2 of the cases. Acid proteinase activity was in the normal range but the activities of beta-glucuronidase and acetylcholine esterase were elevated. The significance of these results in relation to glial cell activity in the early stages of demyelination is discussed.", "contents": "Myelin composition in acute and chronic multiple sclerosis in relation to cerebral lysosomal activity. The neuropathology of 3 cases of acute multiple sclerosis was correlated with biochemical analyses. Astrocytosis was a characteristic feature of the diffuse demyelinating lesions in one case and lymphocytic cuffing characterized the well-defined plaques present in the white matter of the other two cases. No abnormalities were found in the protein or lipid composition of isolated myelin, despite a wide range of recovery. Nevertheless, the gel electrophoretic protein pattern of white matter adjacent to plaque areas showed selective loss of myelin basic protein. Lysosomal acid proteinase and beta-glucuronidase levels were very significantly increased in all white matter samples in which astrocytosis was a major neuropathological feature. Levels were less markedly raised in samples containing discrete active plaques. Enzyme changes were also found in the apparently normal white matter of 2 of the cases. Acid proteinase activity was in the normal range but the activities of beta-glucuronidase and acetylcholine esterase were elevated. The significance of these results in relation to glial cell activity in the early stages of demyelination is discussed.", "PMID": 978213}
{"id": "pubmed23n0032_11575", "title": "Granulomatous angitis of the central nervous system. Case report and review.", "content": "The nineteenth case report of granulomatous angiitis of the central nervous system is described in a 47-year-old patient, who survived 2 years with his disease. Problems with establishing the diagnosis are discussed. No single group of clinical symptoms or laboratory data exist for making a positive diagnosis during life, although a combination of certain nonspecific factors such as mental change, spinal fluid protein elevation and pleocytosis should be present before the diagnosis of granulomatous angiitis is entertained. Special blood tests and immunological studies and contrast procedures, as well as cortical biopsy have been of little value in establishing the diagnosis; instead, a leptomeningeal biopsy may be the procedure of choice. This patient, as well as the others given a trial of steroid treatment, demonstrated some obvious detectable clinical improvement. All patients treated with steroids have survived longer than any untreated patient suggesting that these drugs may be of some benefit.", "contents": "Granulomatous angitis of the central nervous system. Case report and review. The nineteenth case report of granulomatous angiitis of the central nervous system is described in a 47-year-old patient, who survived 2 years with his disease. Problems with establishing the diagnosis are discussed. No single group of clinical symptoms or laboratory data exist for making a positive diagnosis during life, although a combination of certain nonspecific factors such as mental change, spinal fluid protein elevation and pleocytosis should be present before the diagnosis of granulomatous angiitis is entertained. Special blood tests and immunological studies and contrast procedures, as well as cortical biopsy have been of little value in establishing the diagnosis; instead, a leptomeningeal biopsy may be the procedure of choice. This patient, as well as the others given a trial of steroid treatment, demonstrated some obvious detectable clinical improvement. All patients treated with steroids have survived longer than any untreated patient suggesting that these drugs may be of some benefit.", "PMID": 978214}
{"id": "pubmed23n0032_11576", "title": "Hyperphenylalaninaemia and experimental allergic encephalomyelitis.", "content": "Previous suggestions to the effect that a reduced proportion of long chain and unsaturated fatty acids in the CNS as induced by experimental hyperphenylalaninaemia in young rats may alter the biochemical reactivity and stability of the myelin have been examined using experimental allergic encephalomyelitis (EAE). Lewis rats treated chronically with phenylalanine during development showed a higher susceptibility to EAE and a more severe course of the disease than their medium-treated litter mates. The possible implications of this observation for EAE as an experimental model of multiple sclerosis (MS) is discussed briefly in the light of the decreased levels of unsaturated fatty acids found in the CNS of MS patients.", "contents": "Hyperphenylalaninaemia and experimental allergic encephalomyelitis. Previous suggestions to the effect that a reduced proportion of long chain and unsaturated fatty acids in the CNS as induced by experimental hyperphenylalaninaemia in young rats may alter the biochemical reactivity and stability of the myelin have been examined using experimental allergic encephalomyelitis (EAE). Lewis rats treated chronically with phenylalanine during development showed a higher susceptibility to EAE and a more severe course of the disease than their medium-treated litter mates. The possible implications of this observation for EAE as an experimental model of multiple sclerosis (MS) is discussed briefly in the light of the decreased levels of unsaturated fatty acids found in the CNS of MS patients.", "PMID": 978215}
{"id": "pubmed23n0032_11577", "title": "Microdissection of peripheral nerve: collagen and lipid distribution with morphological correlation.", "content": "Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by \"endoneurial plucking\", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.", "contents": "Microdissection of peripheral nerve: collagen and lipid distribution with morphological correlation. Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by \"endoneurial plucking\", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.", "PMID": 978216}
{"id": "pubmed23n0032_11578", "title": "Morphological observations on motor end-plates in rabbits with experimental myasthenia.", "content": "The morphology of motor end-plates in rabbits immunized with Torpedo nicotinic acetylcholine receptor (nAChR) has been studied by light and electron microscopy. Rabbits were studied either after one period of paralysis, some in parallel with electrophysiological recordings of MEPPs and EPPs and of Naja naja alpha-neurotoxin binding properties or after recovery followed by a second paralysis. Changes in the sub-neural apparatus were noted after cholinesterase staining only in the latter group. Ultrastructurally, however, most end-plates in both groups contained a wide range of abnormalities. Many were similar in appearance to those observed in human myasthenia gravis (MG). This further supports the theory that immunized rabbits can be used as a model for myasthenia gravis. In the rabbits with 1 period of paralysis an acute stage of influence on the neuromuscular junction seemed to be present while simplified motor end-plates typical for human MG were mostly found in rabbits with 2 periods of paralysis. Short post-synaptic folds in conjugation with thickeneed membrane-bound vesicles at their tops, inside the basement membrane, were frequently observed. These were interpreted as if the crests of the folds containing nAChR had degenerated and had been budded off. If so, a large number of receptor sites had been lost which would be one possible explanation for the lowered capacity of the muscles to bind Naja naja alpha-neurotoxin. Membrane thickenings with projections and striations were interpreted as reflecting ACh receptors and were observed in the post-junctional membrane without proximity to the nerve terminal. The degeneration of the top of the post-synaptic folds and the occurrence of receptors at other locations within the motor end-plate will result in a widened distance between the nerve terminal and the receptors, which can explain previous interpretations of a presynaptic defect in MG.", "contents": "Morphological observations on motor end-plates in rabbits with experimental myasthenia. The morphology of motor end-plates in rabbits immunized with Torpedo nicotinic acetylcholine receptor (nAChR) has been studied by light and electron microscopy. Rabbits were studied either after one period of paralysis, some in parallel with electrophysiological recordings of MEPPs and EPPs and of Naja naja alpha-neurotoxin binding properties or after recovery followed by a second paralysis. Changes in the sub-neural apparatus were noted after cholinesterase staining only in the latter group. Ultrastructurally, however, most end-plates in both groups contained a wide range of abnormalities. Many were similar in appearance to those observed in human myasthenia gravis (MG). This further supports the theory that immunized rabbits can be used as a model for myasthenia gravis. In the rabbits with 1 period of paralysis an acute stage of influence on the neuromuscular junction seemed to be present while simplified motor end-plates typical for human MG were mostly found in rabbits with 2 periods of paralysis. Short post-synaptic folds in conjugation with thickeneed membrane-bound vesicles at their tops, inside the basement membrane, were frequently observed. These were interpreted as if the crests of the folds containing nAChR had degenerated and had been budded off. If so, a large number of receptor sites had been lost which would be one possible explanation for the lowered capacity of the muscles to bind Naja naja alpha-neurotoxin. Membrane thickenings with projections and striations were interpreted as reflecting ACh receptors and were observed in the post-junctional membrane without proximity to the nerve terminal. The degeneration of the top of the post-synaptic folds and the occurrence of receptors at other locations within the motor end-plate will result in a widened distance between the nerve terminal and the receptors, which can explain previous interpretations of a presynaptic defect in MG.", "PMID": 978217}
{"id": "pubmed23n0032_11579", "title": "Immunological observations on patients with Wilson's disease.", "content": "In 19 patients with Wilson's disease we found an increased humoral immune response, i.e. a higher level of IgG and IgM, a higher titre of antibodies against Kunin's CA antigen and a depressed cell-mediated immunity i.e. a lower response to DNCB and E. coli in skin tests, lower lymphocyte transformation when stimulated by Con A, PPD, Candida albicans and streptokinase and a lower production of macrophage migration inhibition factor. The changes observed in the group of patients with liver cirrhosis caused by other facotrs than Wilson's disease were similar but less pronounced. We also found that leukocytes of patients with Wilson's disease have an impaired bactericidal activity and that copper ions have an inhibitory effect on some tests for cell-mediated immunity. It seems probable that immunological abnormalities in Wilson's disease are caused by liver cirrhosis but we cannot exclude an inhibitory effect of copper ions upon the immune response and an associated effect upon leukocyte metabolism.", "contents": "Immunological observations on patients with Wilson's disease. In 19 patients with Wilson's disease we found an increased humoral immune response, i.e. a higher level of IgG and IgM, a higher titre of antibodies against Kunin's CA antigen and a depressed cell-mediated immunity i.e. a lower response to DNCB and E. coli in skin tests, lower lymphocyte transformation when stimulated by Con A, PPD, Candida albicans and streptokinase and a lower production of macrophage migration inhibition factor. The changes observed in the group of patients with liver cirrhosis caused by other facotrs than Wilson's disease were similar but less pronounced. We also found that leukocytes of patients with Wilson's disease have an impaired bactericidal activity and that copper ions have an inhibitory effect on some tests for cell-mediated immunity. It seems probable that immunological abnormalities in Wilson's disease are caused by liver cirrhosis but we cannot exclude an inhibitory effect of copper ions upon the immune response and an associated effect upon leukocyte metabolism.", "PMID": 978218}
{"id": "pubmed23n0032_11580", "title": "The effect of hypocapnia and change of blood pressure on cerebral blood flow in men with cervical spinal cord transection.", "content": "Cerebral blood flow (CBF) was measured by a 133Xe inhalation technique in 8 patients with chronic high spinal cord injuries. Six patients had a physiologically complete cervical cord transection and 1 subject had an incomplete C4-5 lesion but with evidence of interruption of sympathetic pathways. CBF and arterial blood pressure (BP) were measured in the supine position and then in the sitting, or feet up, position to produce a change of BP. In 4 patients CBF was measured during a suction manoeuvre applied to the lower half of the body to produce a fall of BP. There was no significant change of CBF in the patients during hypo- and hypertension. The response of CBF to hyperventilation for 5 min was measured in the supine position and did not differ significantly from that of 13 normal \"control\" subjects and 1 patient with a lesion at T2-3. It is concluded that the responses of the cerebral circulation to change of blood pressure and to hypocapnia are normal in patients with high spinal cord transection. The mechanisms involved in these responses are therefore independent of control via cervical sympathetic pathways.", "contents": "The effect of hypocapnia and change of blood pressure on cerebral blood flow in men with cervical spinal cord transection. Cerebral blood flow (CBF) was measured by a 133Xe inhalation technique in 8 patients with chronic high spinal cord injuries. Six patients had a physiologically complete cervical cord transection and 1 subject had an incomplete C4-5 lesion but with evidence of interruption of sympathetic pathways. CBF and arterial blood pressure (BP) were measured in the supine position and then in the sitting, or feet up, position to produce a change of BP. In 4 patients CBF was measured during a suction manoeuvre applied to the lower half of the body to produce a fall of BP. There was no significant change of CBF in the patients during hypo- and hypertension. The response of CBF to hyperventilation for 5 min was measured in the supine position and did not differ significantly from that of 13 normal \"control\" subjects and 1 patient with a lesion at T2-3. It is concluded that the responses of the cerebral circulation to change of blood pressure and to hypocapnia are normal in patients with high spinal cord transection. The mechanisms involved in these responses are therefore independent of control via cervical sympathetic pathways.", "PMID": 978219}
{"id": "pubmed23n0032_11581", "title": "On the development of CNS lesions in natural canine distemper encephalomyelitis.", "content": "Acute lesions within spinal cord white matter have been studied by light and electron microscopy in 3 dogs suffering from the acute form of canine distemper encephalomyelitis (CDE). Prominent features of these lesions were viral inclusions, giant cell formation, cellular degeneration, myelin breakdown and phagocytic activity by cells believed to be derived from local glia. The viral inclusions occurred in giant cells, many astrocytes, macrophages and occasional oligodendroglia. Only suggestions of active viral replication from cell membranes were present. On the basis of the above features, these CDE lesions were classed as being acute. Perivascular inflammation and parenchymal invasion by haematogenous cells were lacking. However, older, gliotic, demyelinated lesions were always associated with inflammation. The pattern of demyelination in acute CDE lesions differed from those seen in other conditions, in particular the autoimmune demyelinating diseases. In acute CDE lesions, individual fibres became separated from others by rings of cells, the processes of which systematically stripped the myelin from the outer layers of the sheath inwards until a naked segment of axon remained. Some of the macrophages were recognisable as astroglia. Elsewhere, unequivocal astrocytes containing myelin debris were common. The results suggest that inflammation in acute CDE lesions is not a primary event, and that viral invasion causes breakdown of tissue which is accompanied pari passu by myelin destruction. The latter might be related to the non-specific release of host factors (viz. hydrolytic enzymes) or humoral factors during the cellular degeneration. Local cells appeared to participate in the process of myelin phagocytosis. Overt inflammation and damage by haematogenous cells were features only of chronic lesions and have been described previously in studies on chronic CDE lesions. The results are interpreted in terms of their relevance to the study of human subacute sclerosing panencephalitis, of which CDE is considered the animal analogue, and multiple sclerosis, the paradigm of the human demyelinating diseases.", "contents": "On the development of CNS lesions in natural canine distemper encephalomyelitis. Acute lesions within spinal cord white matter have been studied by light and electron microscopy in 3 dogs suffering from the acute form of canine distemper encephalomyelitis (CDE). Prominent features of these lesions were viral inclusions, giant cell formation, cellular degeneration, myelin breakdown and phagocytic activity by cells believed to be derived from local glia. The viral inclusions occurred in giant cells, many astrocytes, macrophages and occasional oligodendroglia. Only suggestions of active viral replication from cell membranes were present. On the basis of the above features, these CDE lesions were classed as being acute. Perivascular inflammation and parenchymal invasion by haematogenous cells were lacking. However, older, gliotic, demyelinated lesions were always associated with inflammation. The pattern of demyelination in acute CDE lesions differed from those seen in other conditions, in particular the autoimmune demyelinating diseases. In acute CDE lesions, individual fibres became separated from others by rings of cells, the processes of which systematically stripped the myelin from the outer layers of the sheath inwards until a naked segment of axon remained. Some of the macrophages were recognisable as astroglia. Elsewhere, unequivocal astrocytes containing myelin debris were common. The results suggest that inflammation in acute CDE lesions is not a primary event, and that viral invasion causes breakdown of tissue which is accompanied pari passu by myelin destruction. The latter might be related to the non-specific release of host factors (viz. hydrolytic enzymes) or humoral factors during the cellular degeneration. Local cells appeared to participate in the process of myelin phagocytosis. Overt inflammation and damage by haematogenous cells were features only of chronic lesions and have been described previously in studies on chronic CDE lesions. The results are interpreted in terms of their relevance to the study of human subacute sclerosing panencephalitis, of which CDE is considered the animal analogue, and multiple sclerosis, the paradigm of the human demyelinating diseases.", "PMID": 978220}
{"id": "pubmed23n0032_11582", "title": "Spinal muscle in scoliosis. Part 1. Histology and histochemistry.", "content": "Histological and histochemical techniques were employed in a study of the spinal muscles from 35 adolescent scoliotic patients. Many abnormalities were found and, in general, the changes were indicative of denervation and neuropathy. None of the abnormalities were found at any one site more frequently than at any other, nor were they more often seen on one side or the other. None of the patients examined were normal at all sites of biopsy. These findings provide evidence of a neuropathic process being involved in the aetiology or progression of scoliosis. So-called \"idiopathic\" scoliosis may, perhaps, be the result of an otherwise sub-clinical lower motor neurone disorder.", "contents": "Spinal muscle in scoliosis. Part 1. Histology and histochemistry. Histological and histochemical techniques were employed in a study of the spinal muscles from 35 adolescent scoliotic patients. Many abnormalities were found and, in general, the changes were indicative of denervation and neuropathy. None of the abnormalities were found at any one site more frequently than at any other, nor were they more often seen on one side or the other. None of the patients examined were normal at all sites of biopsy. These findings provide evidence of a neuropathic process being involved in the aetiology or progression of scoliosis. So-called \"idiopathic\" scoliosis may, perhaps, be the result of an otherwise sub-clinical lower motor neurone disorder.", "PMID": 978221}
{"id": "pubmed23n0032_11583", "title": "Spinal muscle in scoliosis. Part 2. The proportion and size of type 1 and type 2 skeletal muscle fibres measured using a computer-controlled microscope.", "content": "The development of the computer-controlled microscope system described has made it possible to estimate accurately the contribution made to the muscle fibre population by both Type 1 and Type 2 fibres. This method is more convenient, and at least as accurate as manual methods. It is also possible to measure the size of the individual fibres using this system. The system has been applied to this study of the spinal muscles in scoliosis. The results show that there is a difference in the ratio of Type 1 to Type 2 fibres in the spinal muscles on the 2 sides of the curve. The difference is found only at the apex of the curve. The results demonstrate that there are more Type 2 fibres on the concavity of the curve at the level of the apex. This difference is due to a disparity in the number of each fibre type rather than a difference in their relative sizes. The imbalance of Type 1 fibres at the apex of the curve may provide a sustained muscle pull on the spine so causing a curve convex to that side.", "contents": "Spinal muscle in scoliosis. Part 2. The proportion and size of type 1 and type 2 skeletal muscle fibres measured using a computer-controlled microscope. The development of the computer-controlled microscope system described has made it possible to estimate accurately the contribution made to the muscle fibre population by both Type 1 and Type 2 fibres. This method is more convenient, and at least as accurate as manual methods. It is also possible to measure the size of the individual fibres using this system. The system has been applied to this study of the spinal muscles in scoliosis. The results show that there is a difference in the ratio of Type 1 to Type 2 fibres in the spinal muscles on the 2 sides of the curve. The difference is found only at the apex of the curve. The results demonstrate that there are more Type 2 fibres on the concavity of the curve at the level of the apex. This difference is due to a disparity in the number of each fibre type rather than a difference in their relative sizes. The imbalance of Type 1 fibres at the apex of the curve may provide a sustained muscle pull on the spine so causing a curve convex to that side.", "PMID": 978222}
{"id": "pubmed23n0032_11584", "title": "Clinical effects of cyclophosphamide in Guillain-Barr\u00e9 polyneuritis.", "content": "(1) Fifteen patients with severe Guillain-Barr\u00e9 polyneuritis in progression were treated with cyclophosphamide in a total of 23 courses. The illnesses in 11 patients were monophasic, 2 were biphasic and 1 was of the chronic, relapsing variety; another was possibly biphasic. Length of illness prior to first course of cyclophosphamide was as follows: less than 1 week, 3; 1-4 weeks, 9; greater than 4 weeks, 3. (2) Progression stopped in association with 21 courses of treatment, in 6 before the treatment course was finished and in 15 an average of 3 days after the last dose. Improvement began during or following 19 courses. (3) Time when progression stopped, time from end of progression to beginning of improvement, and subsequent rate of recovery, suggest that cyclophosphamide made a significant contribution to the recovery processes in 13 patients. (4) Ultimate degree of recovery was not significantly different from that expected in conventionally treated patients with monophasic illnesses. (5) Reversible alopoecia was the only significant complication attributable to cyclophosphamide toxicity. (6) Our experience suggests that further evaluation of cyclophosphamide should include the use of controls and earlier treatment.", "contents": "Clinical effects of cyclophosphamide in Guillain-Barr\u00e9 polyneuritis. (1) Fifteen patients with severe Guillain-Barr\u00e9 polyneuritis in progression were treated with cyclophosphamide in a total of 23 courses. The illnesses in 11 patients were monophasic, 2 were biphasic and 1 was of the chronic, relapsing variety; another was possibly biphasic. Length of illness prior to first course of cyclophosphamide was as follows: less than 1 week, 3; 1-4 weeks, 9; greater than 4 weeks, 3. (2) Progression stopped in association with 21 courses of treatment, in 6 before the treatment course was finished and in 15 an average of 3 days after the last dose. Improvement began during or following 19 courses. (3) Time when progression stopped, time from end of progression to beginning of improvement, and subsequent rate of recovery, suggest that cyclophosphamide made a significant contribution to the recovery processes in 13 patients. (4) Ultimate degree of recovery was not significantly different from that expected in conventionally treated patients with monophasic illnesses. (5) Reversible alopoecia was the only significant complication attributable to cyclophosphamide toxicity. (6) Our experience suggests that further evaluation of cyclophosphamide should include the use of controls and earlier treatment.", "PMID": 978223}
{"id": "pubmed23n0032_11585", "title": "Chronic neurologic disease in Theiler's virus infection of SJL/J mice.", "content": "This study demonstrates that most SJL/J mice inoculated intracerebrally (IC) with 1000 suckling mouse 50% mean lethal doses of Theiler's encephalomyelitis virus (TMEV) develop flaccid paralysis 10-21 days after infection when there is acute spinal cord gray matter involvement (early disease). Surviving mice later develop a distinctive chronic neurologic disorder which is associated with marked mononuclear cell infiltrates and active demyelination in spinal cord white matter (late disease). Moreover, about one-fourth of infected animals only develop signs of late disease which may begin after an incubation period as long as 2 and a half months. Affected mice are less active, incontinent, and have a waddling, spastic gait. Minimal stimulation induces prolonged extensor spasms of all limbs. These late-developing manifestations of chronic TMEV infection are progressive and clinical remissions have not been observed. The effect of persistent CNS infection on general development was monitored by weekly measurement of body weight; however, the growth of chronically-infected mice was found to parallel that of control animals.", "contents": "Chronic neurologic disease in Theiler's virus infection of SJL/J mice. This study demonstrates that most SJL/J mice inoculated intracerebrally (IC) with 1000 suckling mouse 50% mean lethal doses of Theiler's encephalomyelitis virus (TMEV) develop flaccid paralysis 10-21 days after infection when there is acute spinal cord gray matter involvement (early disease). Surviving mice later develop a distinctive chronic neurologic disorder which is associated with marked mononuclear cell infiltrates and active demyelination in spinal cord white matter (late disease). Moreover, about one-fourth of infected animals only develop signs of late disease which may begin after an incubation period as long as 2 and a half months. Affected mice are less active, incontinent, and have a waddling, spastic gait. Minimal stimulation induces prolonged extensor spasms of all limbs. These late-developing manifestations of chronic TMEV infection are progressive and clinical remissions have not been observed. The effect of persistent CNS infection on general development was monitored by weekly measurement of body weight; however, the growth of chronically-infected mice was found to parallel that of control animals.", "PMID": 978224}
{"id": "pubmed23n0032_11586", "title": "Analysis of serum immunoglobulins in hereditary sensory radicular neuropathy.", "content": "Serum and salivary immunoglobulins were studied in 11 members of a family including 3 patients with hereditary sensory radicular neuropathy (HSRN) in 1 of whom serum immunoglobulins were measured over a period of about 6 years. It was confirmed that serum IgA and IgG levels rose as the disease progressed. Although our results do not agree, in part, with the findings of Whitaker et al. (1974) who reported an increase in IgA alone, the present study suggested that such dysimmunoglobulinaemia deserves further study. Whether dysimmunoglobulinaemia is a primary phenomenon related to the development of HSRN or simply a secondary change remains to be further explored.", "contents": "Analysis of serum immunoglobulins in hereditary sensory radicular neuropathy. Serum and salivary immunoglobulins were studied in 11 members of a family including 3 patients with hereditary sensory radicular neuropathy (HSRN) in 1 of whom serum immunoglobulins were measured over a period of about 6 years. It was confirmed that serum IgA and IgG levels rose as the disease progressed. Although our results do not agree, in part, with the findings of Whitaker et al. (1974) who reported an increase in IgA alone, the present study suggested that such dysimmunoglobulinaemia deserves further study. Whether dysimmunoglobulinaemia is a primary phenomenon related to the development of HSRN or simply a secondary change remains to be further explored.", "PMID": 978225}
{"id": "pubmed23n0032_11587", "title": "Two patterns of results in polyneuropathies investigated with the H reflex. Correlation between proximal and distal conduction velocities.", "content": "The Hoffman (H) reflex of the soleus muscle has been used to study peripheral neurological disorders in 79 cases of chronic renal insufficiency prior to the initiation of haemodialysis, and in 94 cases of chronic alcoholism. In both groups, care was taken to ensure that the subjects examined could not be suspected of any other type of neuropathy. The results have been compared with those obtained in 42 normal subjects. The nerve conduction velocity in the reflex pathway was evaluated by an H index formula in which the conduction time (interval between M and H responses) is related to the length of the nervous pathway (a function of the subject's height). This method demonstrated a marked reduction in the conduction velocity of the proximal parts of the peripheral nerves, in both uraemic and alcoholic subjects. The maximal amplitude of the reflex response was evaluated in relation to the maximal amplitude of the direct motor response (H max/M max). It was shown that this ratio diminishes very greatly and very rapidly in the course of alcoholic polyneuritis, while, at comparable conduction velocities, the amplitude of the reflex response is much less changed in chronic renal insufficiency. These results prove that there is a noteworthy difference between uraemic and alcoholic polyneuropathy. Its significance is discussed in the context of currently knwon histopathological and electrophysiological data on the polyneuritides. From a practical point of view, the H reflex appears to be an effective tool for the investigation of peripheral neuropathy in man.", "contents": "Two patterns of results in polyneuropathies investigated with the H reflex. Correlation between proximal and distal conduction velocities. The Hoffman (H) reflex of the soleus muscle has been used to study peripheral neurological disorders in 79 cases of chronic renal insufficiency prior to the initiation of haemodialysis, and in 94 cases of chronic alcoholism. In both groups, care was taken to ensure that the subjects examined could not be suspected of any other type of neuropathy. The results have been compared with those obtained in 42 normal subjects. The nerve conduction velocity in the reflex pathway was evaluated by an H index formula in which the conduction time (interval between M and H responses) is related to the length of the nervous pathway (a function of the subject's height). This method demonstrated a marked reduction in the conduction velocity of the proximal parts of the peripheral nerves, in both uraemic and alcoholic subjects. The maximal amplitude of the reflex response was evaluated in relation to the maximal amplitude of the direct motor response (H max/M max). It was shown that this ratio diminishes very greatly and very rapidly in the course of alcoholic polyneuritis, while, at comparable conduction velocities, the amplitude of the reflex response is much less changed in chronic renal insufficiency. These results prove that there is a noteworthy difference between uraemic and alcoholic polyneuropathy. Its significance is discussed in the context of currently knwon histopathological and electrophysiological data on the polyneuritides. From a practical point of view, the H reflex appears to be an effective tool for the investigation of peripheral neuropathy in man.", "PMID": 978226}
{"id": "pubmed23n0032_11588", "title": "Neurochemical findings in a perinatal sudanophilic leukodystrophy rich in steryl ester.", "content": "Neurochemical and neuropathological studies have been made of a 10-day-old child who suffered from a sudanophilic leukodystrophy. The brain white matter contained abundant sudanophilic material. The patient's grey matter total cholesterol content was 30% higher than whole brain tissue derived from a comparable control. White matter cholesterol content was more than double the control value. Nearly 80% of the white matter cholesterol was esterified. Subcellular fractionation of the white matter resulted in a \"floating fraction\" rich in cholesteryl ester. The steryl ester fatty acid composition was not typical of control tissue or demyelinating tissue. Patient phospholipid fatty acid composition patterns differed from control, but white matter galactolipid fatty acid composition appeared normal. Cholesteryl ester hydrolase activity appeared normal. Myelin and myelin-like fractions, isolated from diseased and normal brain tissue, were of a primitive developing nature but appeared to be comparable. The findings indicate a neonatal sudanophilic leukodystrophy which doubtless began in prenatal life and which was rich in cholesteryl ester. The aetiology of the leukodystrophy is unknown.", "contents": "Neurochemical findings in a perinatal sudanophilic leukodystrophy rich in steryl ester. Neurochemical and neuropathological studies have been made of a 10-day-old child who suffered from a sudanophilic leukodystrophy. The brain white matter contained abundant sudanophilic material. The patient's grey matter total cholesterol content was 30% higher than whole brain tissue derived from a comparable control. White matter cholesterol content was more than double the control value. Nearly 80% of the white matter cholesterol was esterified. Subcellular fractionation of the white matter resulted in a \"floating fraction\" rich in cholesteryl ester. The steryl ester fatty acid composition was not typical of control tissue or demyelinating tissue. Patient phospholipid fatty acid composition patterns differed from control, but white matter galactolipid fatty acid composition appeared normal. Cholesteryl ester hydrolase activity appeared normal. Myelin and myelin-like fractions, isolated from diseased and normal brain tissue, were of a primitive developing nature but appeared to be comparable. The findings indicate a neonatal sudanophilic leukodystrophy which doubtless began in prenatal life and which was rich in cholesteryl ester. The aetiology of the leukodystrophy is unknown.", "PMID": 978227}
{"id": "pubmed23n0032_11589", "title": "Aspects of cortical organization related to the geometry of neurons with intra-cortical axons.", "content": "Using the Golgi method, cells with intra-cortical axons in the visual cortex of young mice were classified according to defined geometrical axonal shapes. This study principally describes a computer technique and its application to the study of neuronal morphology. Neurons were converted in a sequence of three-coordinate points which were stored in digital form on magnetic tape. From the stored data and total real length in space of dendrites and axons was obtained and the results compared in two groups of mice raised under different conditions. Preliminary observations show short axonal lengths in mice raised in darkness. Using Eulerian coordinate transformations, reconstructions of individual neurons and of groups of several neurons and fibres were obtained by generating displays of different views after rotation around the horizontal axis. Reconstructed pictures were compared with their corresponding original drawings in order to describe particular aspects of cortical organization.", "contents": "Aspects of cortical organization related to the geometry of neurons with intra-cortical axons. Using the Golgi method, cells with intra-cortical axons in the visual cortex of young mice were classified according to defined geometrical axonal shapes. This study principally describes a computer technique and its application to the study of neuronal morphology. Neurons were converted in a sequence of three-coordinate points which were stored in digital form on magnetic tape. From the stored data and total real length in space of dendrites and axons was obtained and the results compared in two groups of mice raised under different conditions. Preliminary observations show short axonal lengths in mice raised in darkness. Using Eulerian coordinate transformations, reconstructions of individual neurons and of groups of several neurons and fibres were obtained by generating displays of different views after rotation around the horizontal axis. Reconstructed pictures were compared with their corresponding original drawings in order to describe particular aspects of cortical organization.", "PMID": 978228}
{"id": "pubmed23n0032_11590", "title": "The openings of the central canal in the filum terminale internum of some mammals.", "content": "Openings of the central canal in the filum terminale internum of the rabbit, guinea pig, and rat have been studied by light and electron microscopy. There were two openings in the rabbit, two or three in the guinea pig, and one in the rat. They opened dorsally and were of two types; one type was without a pial covering, the other with a pial covering. In both types, a focal junctional apparatus associated with increased density of the subjacent cytoplasm was observed between the pial cell and the ependymal cell at the margins of the opening, where the basal lamina on the surface of the filum terminale showed a characteristic ending for each species. Injections of india ink into the lateral ventricle of the brain indicated that the cerebrospinal fluid of the ventricular system drains out of the central canal by way of these openings, into the subarachnoid space. Reissner's fibre of the rabbit and rat consisted of a mass of moderately electron dense fibrous material containing many small vesicles in the central canal of the filum terminale. This mass passed through the openings into the subarachnoid space and continued into the subdual space.", "contents": "The openings of the central canal in the filum terminale internum of some mammals. Openings of the central canal in the filum terminale internum of the rabbit, guinea pig, and rat have been studied by light and electron microscopy. There were two openings in the rabbit, two or three in the guinea pig, and one in the rat. They opened dorsally and were of two types; one type was without a pial covering, the other with a pial covering. In both types, a focal junctional apparatus associated with increased density of the subjacent cytoplasm was observed between the pial cell and the ependymal cell at the margins of the opening, where the basal lamina on the surface of the filum terminale showed a characteristic ending for each species. Injections of india ink into the lateral ventricle of the brain indicated that the cerebrospinal fluid of the ventricular system drains out of the central canal by way of these openings, into the subarachnoid space. Reissner's fibre of the rabbit and rat consisted of a mass of moderately electron dense fibrous material containing many small vesicles in the central canal of the filum terminale. This mass passed through the openings into the subarachnoid space and continued into the subdual space.", "PMID": 978229}
{"id": "pubmed23n0032_11591", "title": "A nucleolus-associated coiled body.", "content": "One or two healthy structures frequently have been observed attached to nucleoli in facial motor neurons of the golden hamster. These round-to-oval structures, called \"coiled bodies\", were seen at 15, 19, and 24 days postnatal and in the adult, both in normal neurons and in chromatolytic neurons which had been axotomized 4 days previously. With one exception, the coiled bodies were seen to be attached via fibrillar material to the nucleolar periphery. Although the numbers of coiled bodies may be altered during neuronal maturation and as a result of axon section, the bodies revealed no structural alterations that could be attributed to developmental age or to experimental trauma.", "contents": "A nucleolus-associated coiled body. One or two healthy structures frequently have been observed attached to nucleoli in facial motor neurons of the golden hamster. These round-to-oval structures, called \"coiled bodies\", were seen at 15, 19, and 24 days postnatal and in the adult, both in normal neurons and in chromatolytic neurons which had been axotomized 4 days previously. With one exception, the coiled bodies were seen to be attached via fibrillar material to the nucleolar periphery. Although the numbers of coiled bodies may be altered during neuronal maturation and as a result of axon section, the bodies revealed no structural alterations that could be attributed to developmental age or to experimental trauma.", "PMID": 978230}
{"id": "pubmed23n0032_11592", "title": "Acetylcholinesterase activity of synaptic structures in the spinal trigeminal nucleus.", "content": "The electron microscope has been used to study the localization of acetylcholinesterase (AChE) activity in the spinal trigeminal nucleus of normal cats with special emphasis on the distribution near synaptic structures. Reaction product is found around both round and flattened synaptic vesicle-containing axon terminals, particularly in synaptic clefts and often specifically associated with the presynaptic, or less frequently the postsynaptic membrane. The presence of reaction product at these specific sites suggests that these are areas of high AChE activity and that acetylcholine may be important in neurotransmission in these regions.", "contents": "Acetylcholinesterase activity of synaptic structures in the spinal trigeminal nucleus. The electron microscope has been used to study the localization of acetylcholinesterase (AChE) activity in the spinal trigeminal nucleus of normal cats with special emphasis on the distribution near synaptic structures. Reaction product is found around both round and flattened synaptic vesicle-containing axon terminals, particularly in synaptic clefts and often specifically associated with the presynaptic, or less frequently the postsynaptic membrane. The presence of reaction product at these specific sites suggests that these are areas of high AChE activity and that acetylcholine may be important in neurotransmission in these regions.", "PMID": 978231}
{"id": "pubmed23n0032_11593", "title": "Potential of Schwann cells from unmyelinated nerves to produce myelin: a quantitative ultrastructural and radiographic study.", "content": "In adult mice, most fibres in the cervical sympathetic trunk (CST) are unmyelinated whereas a large proportion of sural nerve fibres are myelinated. This study of nerve grafts in syngeneic mice was designed to determine if Schwann cells originating from the unmyelinated CST would produce myelin when in contact with regenerating axons of the sural nerve. Quantitative microscopy of triated thymidine-labelled CST segments grafted to unlabelled sural nerve stumps revealed that, one month after grafting, previously unmyelinated grafts contained many myelinated fibres. By phase and electron microscope radioautography, nearly 40% of the myelin-producing cells in the reinnervated graft were shown to have originated in the unmyelinated CST. These findings indicate that Schwann cells originating from unmyelinated fibres are able to differentiate into myelin producing cells.", "contents": "Potential of Schwann cells from unmyelinated nerves to produce myelin: a quantitative ultrastructural and radiographic study. In adult mice, most fibres in the cervical sympathetic trunk (CST) are unmyelinated whereas a large proportion of sural nerve fibres are myelinated. This study of nerve grafts in syngeneic mice was designed to determine if Schwann cells originating from the unmyelinated CST would produce myelin when in contact with regenerating axons of the sural nerve. Quantitative microscopy of triated thymidine-labelled CST segments grafted to unlabelled sural nerve stumps revealed that, one month after grafting, previously unmyelinated grafts contained many myelinated fibres. By phase and electron microscope radioautography, nearly 40% of the myelin-producing cells in the reinnervated graft were shown to have originated in the unmyelinated CST. These findings indicate that Schwann cells originating from unmyelinated fibres are able to differentiate into myelin producing cells.", "PMID": 978232}
{"id": "pubmed23n0032_11594", "title": "Ultrastructural study of plasma membrane GM1 in neuroectodermal cells using cholera-peroxidase.", "content": "Cholera toxin was coupled to peroxidase to yield a highly specific marker for GM1 gangliosides. Study of embryonic brain cells in culture revealed intense binding of cholera-peroxidase by plasma membranes of both neurons and glial cells. In contrast, long-term monolayer glioblastoma cultures, including one producing C-type virus, revealed virtually no labelling of their plasma membranes. Such cells were shown to be capable of incorporating exogenously applied GM1 into their plasma membranes. Studies with fixed brain and synaptosomal fractions were in accord with results on embryonic brain cells in culture, and autoradiographic findings with 125I cholera supported observations made utilizing cholera-peroxidase. From our studies there is some indication that long-term propagation in vitro alters the plasma membrane GM1.", "contents": "Ultrastructural study of plasma membrane GM1 in neuroectodermal cells using cholera-peroxidase. Cholera toxin was coupled to peroxidase to yield a highly specific marker for GM1 gangliosides. Study of embryonic brain cells in culture revealed intense binding of cholera-peroxidase by plasma membranes of both neurons and glial cells. In contrast, long-term monolayer glioblastoma cultures, including one producing C-type virus, revealed virtually no labelling of their plasma membranes. Such cells were shown to be capable of incorporating exogenously applied GM1 into their plasma membranes. Studies with fixed brain and synaptosomal fractions were in accord with results on embryonic brain cells in culture, and autoradiographic findings with 125I cholera supported observations made utilizing cholera-peroxidase. From our studies there is some indication that long-term propagation in vitro alters the plasma membrane GM1.", "PMID": 978233}
{"id": "pubmed23n0032_11595", "title": "Morphological aspects of the elimination of polyneuronal innervation of skeletal muscle fibres in newborn rats.", "content": "The ultrastructure of neuromuscular junctions of rat soleus muscles 1-40 days postnatally was examined for possible morphological correlates of the transient polyneuronal innervation which is present in newborn rats. Several vesicle-laden profiles of terminal axons are seen to contact each muscle fiber up to 8 days postnatally. Axon terminals often lie close together, without Schwann cell intervention. Between days 8 and 16 the number of profiles of terminals on each muscle fibre is reduced, and both Schwann cells and ridge-like extensions of muscle fibre cytoplasm intervene between and separate axon terminals. No signs of degenerating intramuscular axons or axon terminals could be found. It is suggested that the redundant terminals are eliminated by retraction into the parent axons. This process is apparently accomplished without any morphological signs of degeneration.", "contents": "Morphological aspects of the elimination of polyneuronal innervation of skeletal muscle fibres in newborn rats. The ultrastructure of neuromuscular junctions of rat soleus muscles 1-40 days postnatally was examined for possible morphological correlates of the transient polyneuronal innervation which is present in newborn rats. Several vesicle-laden profiles of terminal axons are seen to contact each muscle fiber up to 8 days postnatally. Axon terminals often lie close together, without Schwann cell intervention. Between days 8 and 16 the number of profiles of terminals on each muscle fibre is reduced, and both Schwann cells and ridge-like extensions of muscle fibre cytoplasm intervene between and separate axon terminals. No signs of degenerating intramuscular axons or axon terminals could be found. It is suggested that the redundant terminals are eliminated by retraction into the parent axons. This process is apparently accomplished without any morphological signs of degeneration.", "PMID": 978234}
{"id": "pubmed23n0032_11596", "title": "Peripheral nerve glycoproteins and myelin fine structure during development of rat sciatic nerve.", "content": "Developmental changes in relative amounts of peripheral nerve proteins and glycoproteins have been correlated with the degree of morphological myelination at various ages during the first 25 postnatal days in rat sciatic nerve. At birth there is virtually no major myelin glycoprotein (P0), but there is a protein which migrates to the same point on sodium dodecyl sulphate (SDS) polyacrylamide gels as the small myelin basic protein (P2). During the time myelin is being formed in the nerve, the P0 protein increases and the P2 protein appears to decrease in relative amount in the nerve. The accumulation of P0 protein in the nerve correlates extremely well with the degree of myelination in sciatic nerve. At 4-6 days postnatal, smooth membrane profiles are observed which are located within axons and in the inner Schwann cell cytoplasm. Such profiles are also observed to fuse with the axolemma-Schwann cell interface. The profiles may represent membrane material being added to or deleted from the axolemma or myelin during myelination.", "contents": "Peripheral nerve glycoproteins and myelin fine structure during development of rat sciatic nerve. Developmental changes in relative amounts of peripheral nerve proteins and glycoproteins have been correlated with the degree of morphological myelination at various ages during the first 25 postnatal days in rat sciatic nerve. At birth there is virtually no major myelin glycoprotein (P0), but there is a protein which migrates to the same point on sodium dodecyl sulphate (SDS) polyacrylamide gels as the small myelin basic protein (P2). During the time myelin is being formed in the nerve, the P0 protein increases and the P2 protein appears to decrease in relative amount in the nerve. The accumulation of P0 protein in the nerve correlates extremely well with the degree of myelination in sciatic nerve. At 4-6 days postnatal, smooth membrane profiles are observed which are located within axons and in the inner Schwann cell cytoplasm. Such profiles are also observed to fuse with the axolemma-Schwann cell interface. The profiles may represent membrane material being added to or deleted from the axolemma or myelin during myelination.", "PMID": 978235}
{"id": "pubmed23n0032_11597", "title": "Activity and excitability to electrical current of cortical auditory receptive neurons of awake cats as affected by stimulus association.", "content": "1. Unit activity and excitability were studied at the midlateral and suprasylvian cortex of naive, blink-conditioned and \"randomization\" cats. The latter received the same CS and US as did the conditioned animals, but in random temporal order and with random intertrial intervals with mean comparable to that used for conditioning. The randomization group failed to develop a blink CR. 2. With conditioning, spontaneous and evoked unit discharges were increased above levels found in naive animals. Correspondingly, levels of extracellularly injected current required to elicit a spike discharge were lower in conditioned than in naive animals. In addition, in the conditioned animals, the degree of enhancement of evoked activity and excitability was found to be greatest in the units that responded to the CS, as opposed to units that responded to another auditory stimulus of equal intensity but of no special behavioral significance vis-a-vis the conditioned reflex. 3...", "contents": "Activity and excitability to electrical current of cortical auditory receptive neurons of awake cats as affected by stimulus association. 1. Unit activity and excitability were studied at the midlateral and suprasylvian cortex of naive, blink-conditioned and \"randomization\" cats. The latter received the same CS and US as did the conditioned animals, but in random temporal order and with random intertrial intervals with mean comparable to that used for conditioning. The randomization group failed to develop a blink CR. 2. With conditioning, spontaneous and evoked unit discharges were increased above levels found in naive animals. Correspondingly, levels of extracellularly injected current required to elicit a spike discharge were lower in conditioned than in naive animals. In addition, in the conditioned animals, the degree of enhancement of evoked activity and excitability was found to be greatest in the units that responded to the CS, as opposed to units that responded to another auditory stimulus of equal intensity but of no special behavioral significance vis-a-vis the conditioned reflex. 3...", "PMID": 978236}
{"id": "pubmed23n0032_11598", "title": "Effect of early exposure to patterned sound on unit activity in rat inferior colliculus.", "content": "1. Young rats were exposed to one of two patterns of sounds for 5 h daily during the first 4 mo of life. The up pattern consisted of a tone swept from 6 to 9 kHz in 1 s alternated with a 1-s noise burst. The down pattern differed in that the sweeps were from 9 to 6 kHz. 2. A pattern evoked more spikes, on the average, from units in the inferior colliculus of rats exposed to that pattern than from units in animals exposed to the other pattern. 3. The exposure effect was most pronounced in the unit responses to the noise-burst segment within a pattern suggesting a long-lasting, malleable influence of the tone sweep which defined the pattern. Responses to pattern noise components were less for both exposed groups than for the unexposed controls, suggesting that inhibitory mechanisms were responsible for the pattern discrimination evident in the responses of the exposed unit population. 4. Unit responses in unexposed rats were somewhat more selective for the down pattern so that the resulting shift in response selectivity was relatively more apparent for exposure to the up pattern. 5. While control and down-exposed units generally responded more to both the tone sweep and noise burst in one pattern, a large proportion of the up-exposed unit population continued to favor the down over the up-swept tone, but responded more to the up-pattern noise bursts. This suggests that the unit responses to the noise bursts did not simply reflect prolonged responses to the tone sweeps. 6. No similar effects were seen for units from mothers similarly exposed to the same patterns.", "contents": "Effect of early exposure to patterned sound on unit activity in rat inferior colliculus. 1. Young rats were exposed to one of two patterns of sounds for 5 h daily during the first 4 mo of life. The up pattern consisted of a tone swept from 6 to 9 kHz in 1 s alternated with a 1-s noise burst. The down pattern differed in that the sweeps were from 9 to 6 kHz. 2. A pattern evoked more spikes, on the average, from units in the inferior colliculus of rats exposed to that pattern than from units in animals exposed to the other pattern. 3. The exposure effect was most pronounced in the unit responses to the noise-burst segment within a pattern suggesting a long-lasting, malleable influence of the tone sweep which defined the pattern. Responses to pattern noise components were less for both exposed groups than for the unexposed controls, suggesting that inhibitory mechanisms were responsible for the pattern discrimination evident in the responses of the exposed unit population. 4. Unit responses in unexposed rats were somewhat more selective for the down pattern so that the resulting shift in response selectivity was relatively more apparent for exposure to the up pattern. 5. While control and down-exposed units generally responded more to both the tone sweep and noise burst in one pattern, a large proportion of the up-exposed unit population continued to favor the down over the up-swept tone, but responded more to the up-pattern noise bursts. This suggests that the unit responses to the noise bursts did not simply reflect prolonged responses to the tone sweeps. 6. No similar effects were seen for units from mothers similarly exposed to the same patterns.", "PMID": 978237}
{"id": "pubmed23n0032_11599", "title": "Regulatory actions of human stretch reflex.", "content": "1. The stretch reflex in the elbow flexor musculature was studied in 23 human subjects. The subjects were required to establish an initial force equivalent to 10% maximum at a prescribed initial length; mechanical disturbances delivered at random times increased load force to 15% or reduced it to 5%. We measured arm force, displacement, and EMG (usually biceps); acceleration was calculated from displacement, and average responses from sets of 10 like trials. 2. Modification of the stretch reflex was studied by comparing average responses obtained with different instructions, but with the same disturbance. The usual introductions were \"compensate for arm deflection\" and \"do not intervene voluntarily\". The initial response did not depend on instruction; changes in response that depended on instruction began abruptly after a latent period which ranged from 70 to 320 ms (measured from force and acceleration), depending on conditions and subject. The latency became longer (10-50 ms) and more variable when the subject did not know the direction of disturbance in advance. This and other observations indicate that modifications of the stretch reflex are not produced by servo actions. They are produced by triggered reactions, which occur at both short and long latencies and which have properties resembling the movements produced in a reaction-time task. 3. We confirmed that most subjects can suppress triggered reactions when the instruction calls for no intervention, leaving an unmodified reflex response. This response consists of a compliant deflection of the arm in the direction of the disturbance. 4. The compensatory actions associated with unmodified stretch (and unloading) reflexes were assessed from EMG responses of biceps. During a 300-ms transient phase, EMG changes were notably asymmetric when responses to symmetric disturbances were compared. Increased force stretched biceps and produced a prominent increase in EMG, whereas decreased force allowed biceps to shorten and produced either an EMG decrease of smaller magnitude or an actual increase. These asymmetric reflex actions produced quite symmetric mechanical responses (arm displacements and forces), which implies the existence of and compensation for nonlinear muscle mechanical properties. This result is discussed in relation to the hypothesis that the function of the stretch reflex is to compensate for variations in muscle properties, thus maintaining stiffness. 5. Effective control of muscle length or joint position does not result from servo action by the stretch reflex. Errors in position are corrected only when triggered reactions are superimposed on the reflex response.", "contents": "Regulatory actions of human stretch reflex. 1. The stretch reflex in the elbow flexor musculature was studied in 23 human subjects. The subjects were required to establish an initial force equivalent to 10% maximum at a prescribed initial length; mechanical disturbances delivered at random times increased load force to 15% or reduced it to 5%. We measured arm force, displacement, and EMG (usually biceps); acceleration was calculated from displacement, and average responses from sets of 10 like trials. 2. Modification of the stretch reflex was studied by comparing average responses obtained with different instructions, but with the same disturbance. The usual introductions were \"compensate for arm deflection\" and \"do not intervene voluntarily\". The initial response did not depend on instruction; changes in response that depended on instruction began abruptly after a latent period which ranged from 70 to 320 ms (measured from force and acceleration), depending on conditions and subject. The latency became longer (10-50 ms) and more variable when the subject did not know the direction of disturbance in advance. This and other observations indicate that modifications of the stretch reflex are not produced by servo actions. They are produced by triggered reactions, which occur at both short and long latencies and which have properties resembling the movements produced in a reaction-time task. 3. We confirmed that most subjects can suppress triggered reactions when the instruction calls for no intervention, leaving an unmodified reflex response. This response consists of a compliant deflection of the arm in the direction of the disturbance. 4. The compensatory actions associated with unmodified stretch (and unloading) reflexes were assessed from EMG responses of biceps. During a 300-ms transient phase, EMG changes were notably asymmetric when responses to symmetric disturbances were compared. Increased force stretched biceps and produced a prominent increase in EMG, whereas decreased force allowed biceps to shorten and produced either an EMG decrease of smaller magnitude or an actual increase. These asymmetric reflex actions produced quite symmetric mechanical responses (arm displacements and forces), which implies the existence of and compensation for nonlinear muscle mechanical properties. This result is discussed in relation to the hypothesis that the function of the stretch reflex is to compensate for variations in muscle properties, thus maintaining stiffness. 5. Effective control of muscle length or joint position does not result from servo action by the stretch reflex. Errors in position are corrected only when triggered reactions are superimposed on the reflex response.", "PMID": 978238}
{"id": "pubmed23n0032_11600", "title": "Angiographic study of the growth of intracranial aneurysms.", "content": "The authors have attempted to assess whether there is any standard rate of growth of intracranial aneurysms. Angiographic studies were performed on 67 patients with 82 aneurysms with intervals between angiograms ranging from a few days to 10 years. Examination of these records showed no consistent rate of change in size, possibly partly because of inherent inaccuracies in the method, which are discussed. The clinical reasons for the repeat studies are briefly mentioned. It is concluded that it is unwise to wait and see what may happen to any but perhaps the smallest aneurysm.", "contents": "Angiographic study of the growth of intracranial aneurysms. The authors have attempted to assess whether there is any standard rate of growth of intracranial aneurysms. Angiographic studies were performed on 67 patients with 82 aneurysms with intervals between angiograms ranging from a few days to 10 years. Examination of these records showed no consistent rate of change in size, possibly partly because of inherent inaccuracies in the method, which are discussed. The clinical reasons for the repeat studies are briefly mentioned. It is concluded that it is unwise to wait and see what may happen to any but perhaps the smallest aneurysm.", "PMID": 978239}
{"id": "pubmed23n0032_11601", "title": "Lateral extracavitary approach to traumatic lesions of the thoracic and lumbar spine.", "content": "The lateral extracavitary approach to the spine was used for resection of displaced bpne and disc located anterior to the dura in 62 patients with traumatic lesions of the thoracic and lumbar spine. Fifty-two patients had closed vertebral fractures and 10 had gunshot wounds. The spinal cord was involved in 44 patients, and the cauda equina in 18. A spinal subarachnoid block was demonstrated in 17 of 57 preoperative gas myelograms. Evoked potential recordings, although related to preception of joint rotation, tended to reflect the overall neurological condition and had some prognostic value. Significant improvement followed surgery in 46 patients with incomplete neurological lesions, and one was transiently worse. Before operation 18 patients were able to walk; nine with assistance and nine without. After operation 47 patients were able to walk; 12 with assistance and 35 without. Adequate bladder function was present in 17 patients before surgery, and in 44 after surgery. A laminectomy had been done previously in 16 patients, 11 of whom improved significantly after anterior resection. Spine fusions were required in 26 patients, five of whom had a prior laminectomy. The major factor in the pathogenesis of the incomplete neurological deficit appeared to be distortion of the cord and roots by displaced bone and disc. Consequently, the primary object of treatment was the restoration and maintenance of normal anatomical relationships between the spinal cord or cauda equina and the spinal canal.", "contents": "Lateral extracavitary approach to traumatic lesions of the thoracic and lumbar spine. The lateral extracavitary approach to the spine was used for resection of displaced bpne and disc located anterior to the dura in 62 patients with traumatic lesions of the thoracic and lumbar spine. Fifty-two patients had closed vertebral fractures and 10 had gunshot wounds. The spinal cord was involved in 44 patients, and the cauda equina in 18. A spinal subarachnoid block was demonstrated in 17 of 57 preoperative gas myelograms. Evoked potential recordings, although related to preception of joint rotation, tended to reflect the overall neurological condition and had some prognostic value. Significant improvement followed surgery in 46 patients with incomplete neurological lesions, and one was transiently worse. Before operation 18 patients were able to walk; nine with assistance and nine without. After operation 47 patients were able to walk; 12 with assistance and 35 without. Adequate bladder function was present in 17 patients before surgery, and in 44 after surgery. A laminectomy had been done previously in 16 patients, 11 of whom improved significantly after anterior resection. Spine fusions were required in 26 patients, five of whom had a prior laminectomy. The major factor in the pathogenesis of the incomplete neurological deficit appeared to be distortion of the cord and roots by displaced bone and disc. Consequently, the primary object of treatment was the restoration and maintenance of normal anatomical relationships between the spinal cord or cauda equina and the spinal canal.", "PMID": 978240}
{"id": "pubmed23n0032_11602", "title": "Prediction of mobility gains in patients with cervical spinal cord injuries.", "content": "A measure of mobility for patients with spinal cord injuries is introduced that is very useful in determining patient recovery. The index is used to obtain a prediction equation for motor skills 1 year after injury. Important predictor variables identified include the patient's sex, rectal status, total reflexes, two treatment combinations, motor and sensory neurological history since injury, neurological status, and initial mobility score. Interpretations are made to explain the meaning of the contributions of these variables and show the usefulness of the prediction equation.", "contents": "Prediction of mobility gains in patients with cervical spinal cord injuries. A measure of mobility for patients with spinal cord injuries is introduced that is very useful in determining patient recovery. The index is used to obtain a prediction equation for motor skills 1 year after injury. Important predictor variables identified include the patient's sex, rectal status, total reflexes, two treatment combinations, motor and sensory neurological history since injury, neurological status, and initial mobility score. Interpretations are made to explain the meaning of the contributions of these variables and show the usefulness of the prediction equation.", "PMID": 978241}
{"id": "pubmed23n0032_11603", "title": "Myelotomy for control of mass spasms in paraplegia.", "content": "A new myelotomy knife is described and a procedure, designed to sever certain reflex connections while preserving as many corticospinal connections as possible, is presented. Through intermittent dorsal midline incisions the gray matter lateral to the central canal is severed bilaterally under the microscope from L-1 to S-1. This procedure relieved mass spasms and hyperactive reflexes in 14 paraplegic or tetraplegic patients, but preserved postural reflexes and whatever voluntary motor power the patients had prior to myelotomy. Before myelotomy all patients were bedridden. Afterward nine patients were able to use a wheel chair and five were able to walk with the use of parallel bars or crutches.", "contents": "Myelotomy for control of mass spasms in paraplegia. A new myelotomy knife is described and a procedure, designed to sever certain reflex connections while preserving as many corticospinal connections as possible, is presented. Through intermittent dorsal midline incisions the gray matter lateral to the central canal is severed bilaterally under the microscope from L-1 to S-1. This procedure relieved mass spasms and hyperactive reflexes in 14 paraplegic or tetraplegic patients, but preserved postural reflexes and whatever voluntary motor power the patients had prior to myelotomy. Before myelotomy all patients were bedridden. Afterward nine patients were able to use a wheel chair and five were able to walk with the use of parallel bars or crutches.", "PMID": 978242}
{"id": "pubmed23n0032_11604", "title": "Brain abscess caused by Salmonella typhi. Case report.", "content": "The authors report a rare case of a brain abscess associated with osteomyelitis caused by Salmonella typhi that remained in contact with human tissues for 33 years without losing its virulence.", "contents": "Brain abscess caused by Salmonella typhi. Case report. The authors report a rare case of a brain abscess associated with osteomyelitis caused by Salmonella typhi that remained in contact with human tissues for 33 years without losing its virulence.", "PMID": 978243}
{"id": "pubmed23n0032_11605", "title": "Primary Burkitt's lymphoma of the frontal bone. Case report.", "content": "The author reports a case of recurrent Burkitt's lymphoma in the left frontal region with extradural extension. Craniotomy was performed to relieve rapidly progressive intracranial compression. The patient received cytotoxic treatment postoperatively, with remarkable regression of the tumor.", "contents": "Primary Burkitt's lymphoma of the frontal bone. Case report. The author reports a case of recurrent Burkitt's lymphoma in the left frontal region with extradural extension. Craniotomy was performed to relieve rapidly progressive intracranial compression. The patient received cytotoxic treatment postoperatively, with remarkable regression of the tumor.", "PMID": 978244}
{"id": "pubmed23n0032_11606", "title": "Complications of halo-pelvic traction. Case report.", "content": "When halo-pelvic traction is applied at a rapid rate it may induce cranial nerve palsies. The sixth, ninth, and tenth cranial nerves appear to be the most vulnerable. A proposed etiology is the stretching of these nerves resulting in a compromised blood supply with a consequent temporary paralysis which usually improves within 8 to 10 weeks.", "contents": "Complications of halo-pelvic traction. Case report. When halo-pelvic traction is applied at a rapid rate it may induce cranial nerve palsies. The sixth, ninth, and tenth cranial nerves appear to be the most vulnerable. A proposed etiology is the stretching of these nerves resulting in a compromised blood supply with a consequent temporary paralysis which usually improves within 8 to 10 weeks.", "PMID": 978245}
{"id": "pubmed23n0032_11607", "title": "New 68Ga-labeled skeletal-imaging agents for positron scintigraphy.", "content": "The present skeletal-imaging agents labeled with radiogallium rely upon carrier gallium to augment bone uptake; no gallium-labeled bone-imaging agent free of this disadvantage is available. In attempts to develop such agents, we prepared 68Ga-ethylenediaminetetramethylene phosphonate (68Gs-EDTMP) and 68Ga-diethylenetriaminepentamethylene phosphonate (68Ga-DTPMP) and determined their biologic distributions in rats and dogs. These compounds combine the bone-seeking characteristics of phosphonic acid and the complexing ability of EDTA and DTPA analogs. The chelates are administered without gallium carrier. In rats, 50-60% of the carrier-free dose accumulates in bone at 1 hr after intravenous injection, while 25--30% is excreted through the urine. In dogs, at 3 hr after intravenous injection 35% is found in bone. Although the general patterns of organ distribution of the two 68Ga chelates are similar, 68Ga-EDTMP appears superior because of its faster blood clearance. Bone images obtained with this compound in dogs, using a multidetector positron camera, are presented. The optimum time for imaging was found to be 2.5--3 hr after injection.", "contents": "New 68Ga-labeled skeletal-imaging agents for positron scintigraphy. The present skeletal-imaging agents labeled with radiogallium rely upon carrier gallium to augment bone uptake; no gallium-labeled bone-imaging agent free of this disadvantage is available. In attempts to develop such agents, we prepared 68Ga-ethylenediaminetetramethylene phosphonate (68Gs-EDTMP) and 68Ga-diethylenetriaminepentamethylene phosphonate (68Ga-DTPMP) and determined their biologic distributions in rats and dogs. These compounds combine the bone-seeking characteristics of phosphonic acid and the complexing ability of EDTA and DTPA analogs. The chelates are administered without gallium carrier. In rats, 50-60% of the carrier-free dose accumulates in bone at 1 hr after intravenous injection, while 25--30% is excreted through the urine. In dogs, at 3 hr after intravenous injection 35% is found in bone. Although the general patterns of organ distribution of the two 68Ga chelates are similar, 68Ga-EDTMP appears superior because of its faster blood clearance. Bone images obtained with this compound in dogs, using a multidetector positron camera, are presented. The optimum time for imaging was found to be 2.5--3 hr after injection.", "PMID": 978248}
{"id": "pubmed23n0032_11608", "title": "A new nuclear medicine scintillation camera based on image-intensifier tubes.", "content": "A large-field scintilation camera for nuclear medicine application has recently been developed by Old Delft. The system is based on a large-field image-intensifier tube preceded by a scintillator mosaic. A comparison is made with present state-of-the-art scintillation cameras in terms of modulation transfer function (MTF) and sensitivity. These parameters, which determine the performance of scintillation cameras, are not independent of each other. Therefore, a comparative evaluation should be made under well-defined and identical conditions. The new scintillation camera achieves considerable improvement in image quality. In fact, the intrinsic MTF of the new camera is rather close to unity in the spatial frequency range up to 1 line pair per centimeter (1p/cm). Further improvement would require a fundamentally new approach to gamma imaging, free of the limitations of conventional collimators (e.g., coded-aperture imaging techniques).", "contents": "A new nuclear medicine scintillation camera based on image-intensifier tubes. A large-field scintilation camera for nuclear medicine application has recently been developed by Old Delft. The system is based on a large-field image-intensifier tube preceded by a scintillator mosaic. A comparison is made with present state-of-the-art scintillation cameras in terms of modulation transfer function (MTF) and sensitivity. These parameters, which determine the performance of scintillation cameras, are not independent of each other. Therefore, a comparative evaluation should be made under well-defined and identical conditions. The new scintillation camera achieves considerable improvement in image quality. In fact, the intrinsic MTF of the new camera is rather close to unity in the spatial frequency range up to 1 line pair per centimeter (1p/cm). Further improvement would require a fundamentally new approach to gamma imaging, free of the limitations of conventional collimators (e.g., coded-aperture imaging techniques).", "PMID": 978249}
{"id": "pubmed23n0032_11609", "title": "Procedures for receiving and opening packages containing radioactivity.", "content": "A concise set of procedures is presented to aid laboratory personnel in complying with Part 20.205 of Title 10, Chapter 1, of the Code of Federal Regulations, concerning the receipt and monitoring of radioactive materials. Because these regulations were written for a large and diverse community of licenses, they are difficult to implement on a day-to-day basis. The procedures presented here reduce the regulations to an operationally functional form.", "contents": "Procedures for receiving and opening packages containing radioactivity. A concise set of procedures is presented to aid laboratory personnel in complying with Part 20.205 of Title 10, Chapter 1, of the Code of Federal Regulations, concerning the receipt and monitoring of radioactive materials. Because these regulations were written for a large and diverse community of licenses, they are difficult to implement on a day-to-day basis. The procedures presented here reduce the regulations to an operationally functional form.", "PMID": 978250}
{"id": "pubmed23n0032_11610", "title": "A simplified radiopharmaceutical accountability record-keeping system.", "content": "A comprehensive system has been developed to ensure that a patient receives the proper radiopharmaceutical dose and that records of accountability are conveniently maintained. A radiopharmacy assay/utilization record serves as the control document for source products and derived new products. Color-coded numbered labels are used to maintain accountability among source products, derived products, prescriptions, logbooks, and patient doses. These measures have proved effective in simplifying documentation, minimizing dose-administration errors, and providing for radiopharmaceutical accountability.", "contents": "A simplified radiopharmaceutical accountability record-keeping system. A comprehensive system has been developed to ensure that a patient receives the proper radiopharmaceutical dose and that records of accountability are conveniently maintained. A radiopharmacy assay/utilization record serves as the control document for source products and derived new products. Color-coded numbered labels are used to maintain accountability among source products, derived products, prescriptions, logbooks, and patient doses. These measures have proved effective in simplifying documentation, minimizing dose-administration errors, and providing for radiopharmaceutical accountability.", "PMID": 978251}
{"id": "pubmed23n0032_11611", "title": "Quenching curves: solutions by second-order polynomial regression.", "content": "This paper describes an inexpensive practical approach for the automated computation of quench corrections in beta-scintillation experiments, using a table-top calculator. This approach applies a second-order fit, ax2 + bx + c = y, where x is the channel A/B ratio for known quench standards and y describes efficiency. There is excellent agreement between measured values and the polynomial expression for most experimental A/B ratios.", "contents": "Quenching curves: solutions by second-order polynomial regression. This paper describes an inexpensive practical approach for the automated computation of quench corrections in beta-scintillation experiments, using a table-top calculator. This approach applies a second-order fit, ax2 + bx + c = y, where x is the channel A/B ratio for known quench standards and y describes efficiency. There is excellent agreement between measured values and the polynomial expression for most experimental A/B ratios.", "PMID": 978252}
{"id": "pubmed23n0032_11612", "title": "Color functional images of the cerebral blood flow.", "content": "Functional gamma imaging, in color, was established for regional cerebral blood flow (rCBF) using 133Xe. During 10 min after intracarotid injection of 133Xe in saline, 60 picture frames of the 133Xe clearance curve for the entire hemisphere were obtained. After nine-point smoothing, the rCBF for each of the 4,096 picture elements was calculated by two methods: the half-time method and the height-over-area method. Both the 133Xe clearance half-times and the calculated CBF values were displayed, using 13 steps of color, as functional CBF images of the brain. Images of peak count and total count were also displayed on the same frame of the color television. Forty-six studies, performed on 37 patients with various cerebral disorders, were divided into two types: diffuse and focal. In the diffuse type, a decrease in CBF was noted in cases of normal-pressure hydrocephalus; successful ventriculoperitoneal shunt operations were followed by recovery of CBF. Occlusion of the middle cerebral artery showed up as a wedge-shaped area of decreased CBF, even when the conventional brain scan looked normal. Increased perfusion to a tumor was frequently associated with decreased CBF in the rest of the lateral hemisphere; such a decrease could be improved by surgical removal of the tumor.", "contents": "Color functional images of the cerebral blood flow. Functional gamma imaging, in color, was established for regional cerebral blood flow (rCBF) using 133Xe. During 10 min after intracarotid injection of 133Xe in saline, 60 picture frames of the 133Xe clearance curve for the entire hemisphere were obtained. After nine-point smoothing, the rCBF for each of the 4,096 picture elements was calculated by two methods: the half-time method and the height-over-area method. Both the 133Xe clearance half-times and the calculated CBF values were displayed, using 13 steps of color, as functional CBF images of the brain. Images of peak count and total count were also displayed on the same frame of the color television. Forty-six studies, performed on 37 patients with various cerebral disorders, were divided into two types: diffuse and focal. In the diffuse type, a decrease in CBF was noted in cases of normal-pressure hydrocephalus; successful ventriculoperitoneal shunt operations were followed by recovery of CBF. Occlusion of the middle cerebral artery showed up as a wedge-shaped area of decreased CBF, even when the conventional brain scan looked normal. Increased perfusion to a tumor was frequently associated with decreased CBF in the rest of the lateral hemisphere; such a decrease could be improved by surgical removal of the tumor.", "PMID": 978254}
{"id": "pubmed23n0032_11613", "title": "Origin and location on the oral activity in sequential salivary gland scintigraphy with 99mTc-pertechnetate.", "content": "Conflicting views exist regarding the origin and location of the oral radioactivity seen in salivary gland scintigraphy with 99mTc-pertechnetate. The normal accumulation of oral activity was studied in a series of healthy subjects by sequential scintigraphy (anterior and lateral views) after intravenous injection of 2 mCi of 99mTc-pertechnetate. Ligation of the parotid ducts and/or cannulation of the submandibular ducts, which prevented their secretions from reaching the oral cavity, established that the oral activity was due entirely to radioactive saliva secreted by the parotid and submandibular glands. Pertechnetate mouthwash studies confirmed that radioactive saliva is absorbed to oral mucosa and that adsorption to the lingual mucosa in the major contributor to the oral activity. Pertechnetate uptake in sublingual and minor salivary glands of the oral cavity was not visualized, thereby invalidating scintigraphy in the study of these glands. In the diagnosis of salivary gland disorders, oral activity is a useful indicator of major salivary gland function.", "contents": "Origin and location on the oral activity in sequential salivary gland scintigraphy with 99mTc-pertechnetate. Conflicting views exist regarding the origin and location of the oral radioactivity seen in salivary gland scintigraphy with 99mTc-pertechnetate. The normal accumulation of oral activity was studied in a series of healthy subjects by sequential scintigraphy (anterior and lateral views) after intravenous injection of 2 mCi of 99mTc-pertechnetate. Ligation of the parotid ducts and/or cannulation of the submandibular ducts, which prevented their secretions from reaching the oral cavity, established that the oral activity was due entirely to radioactive saliva secreted by the parotid and submandibular glands. Pertechnetate mouthwash studies confirmed that radioactive saliva is absorbed to oral mucosa and that adsorption to the lingual mucosa in the major contributor to the oral activity. Pertechnetate uptake in sublingual and minor salivary glands of the oral cavity was not visualized, thereby invalidating scintigraphy in the study of these glands. In the diagnosis of salivary gland disorders, oral activity is a useful indicator of major salivary gland function.", "PMID": 978255}
{"id": "pubmed23n0032_11614", "title": "A simplified approach to radionuclide venography: concise communication.", "content": "A simplified method has been developed for performing radionuclide venography. The method makes use of the scintillation camera and a synchronized whole-body scanning bed. This technique permits a more integrated presentation of the data and is performed in conjunction with a standard ventilation-perfusion lung study. The total amount of 99mTc tracer injected is 2 mCi.", "contents": "A simplified approach to radionuclide venography: concise communication. A simplified method has been developed for performing radionuclide venography. The method makes use of the scintillation camera and a synchronized whole-body scanning bed. This technique permits a more integrated presentation of the data and is performed in conjunction with a standard ventilation-perfusion lung study. The total amount of 99mTc tracer injected is 2 mCi.", "PMID": 978256}
{"id": "pubmed23n0032_11615", "title": "Diagnostic scintigraphic sign in epidural hematoma at the vertex: case report.", "content": "Brain scintigraphy was described in a case of vertical (biparasagittal) epidural hematoma confirmed by contrast angiography. The anterior dynamic and static studies showed inferior displacement of the superior sagittal sinus, diagnostic of an epidural collection. Delayed static anterior and lateral images showed an increased vertical uptake with a crescentic and straight inner contour, respectively. A transaxial computed tomographic scan was negative.", "contents": "Diagnostic scintigraphic sign in epidural hematoma at the vertex: case report. Brain scintigraphy was described in a case of vertical (biparasagittal) epidural hematoma confirmed by contrast angiography. The anterior dynamic and static studies showed inferior displacement of the superior sagittal sinus, diagnostic of an epidural collection. Delayed static anterior and lateral images showed an increased vertical uptake with a crescentic and straight inner contour, respectively. A transaxial computed tomographic scan was negative.", "PMID": 978257}
{"id": "pubmed23n0032_11616", "title": "The rim sign in epidural hematoma: case report.", "content": "A case of epidural hematoma of 7 days' duration showed a rim sign in the dynamic and static brain images. The significance and value of a rim sign are discussed.", "contents": "The rim sign in epidural hematoma: case report. A case of epidural hematoma of 7 days' duration showed a rim sign in the dynamic and static brain images. The significance and value of a rim sign are discussed.", "PMID": 978258}
{"id": "pubmed23n0032_11617", "title": "Abnormal rapid-sequence imaging in a patient with subdural empyema: case report.", "content": "Static brain images in a patient with subdural empyema showed findings similar to those of a subdural hematoma. The rapid-sequence study, however, showed increased peripheral activity on the affected side. Since this finding suggested an inflammatory process, further neuroradiologic evaluation and immediate surgical treatment were undertaken. The significance of the rapid-sequence study in subdural empyema has not been reported previously.", "contents": "Abnormal rapid-sequence imaging in a patient with subdural empyema: case report. Static brain images in a patient with subdural empyema showed findings similar to those of a subdural hematoma. The rapid-sequence study, however, showed increased peripheral activity on the affected side. Since this finding suggested an inflammatory process, further neuroradiologic evaluation and immediate surgical treatment were undertaken. The significance of the rapid-sequence study in subdural empyema has not been reported previously.", "PMID": 978259}
{"id": "pubmed23n0032_11618", "title": "Primary and secondary carcinomata with focal nodular hyperplasia in a multinodular thyroid: case report.", "content": "A patient is described whose multinodular thyroid gland was found to have a primary papillary adenocarcinoma, a metastatic renal-cell carcinoma, and focal nodular hyperplasia. To our knowledge, this is the first case report of such an unusual combination. In a patient with known malignancy elsewhere, the possibility that a recent thyroid mass may be a metastasis should be considered.", "contents": "Primary and secondary carcinomata with focal nodular hyperplasia in a multinodular thyroid: case report. A patient is described whose multinodular thyroid gland was found to have a primary papillary adenocarcinoma, a metastatic renal-cell carcinoma, and focal nodular hyperplasia. To our knowledge, this is the first case report of such an unusual combination. In a patient with known malignancy elsewhere, the possibility that a recent thyroid mass may be a metastasis should be considered.", "PMID": 978260}
{"id": "pubmed23n0032_11619", "title": "\"Owl eye\" sign in thyroid nodule of papillary carcinoma: case report.", "content": "A cold area within a benign autonomous thyroid nodule comprises the \"owl eye\" sign. A case of thyroid carcinoma is presented whose scan appearance simulates that of a benign autonomous nodule with the \"owl eye\" sign.", "contents": "\"Owl eye\" sign in thyroid nodule of papillary carcinoma: case report. A cold area within a benign autonomous thyroid nodule comprises the \"owl eye\" sign. A case of thyroid carcinoma is presented whose scan appearance simulates that of a benign autonomous nodule with the \"owl eye\" sign.", "PMID": 978261}
{"id": "pubmed23n0032_11620", "title": "Technetium-99m labeling of human monocytes for chemotactic studies.", "content": "Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of 99mTc-labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.", "contents": "Technetium-99m labeling of human monocytes for chemotactic studies. Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of 99mTc-labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.", "PMID": 978262}
{"id": "pubmed23n0032_11621", "title": "Localization of skeletal-imaging 99mTc chelates in dead cells in tissue culture: concise communication.", "content": "Screening new agents for imaging myocardial infarcts and investigating the mechanism of cellular uptake and subcellular localization of these agents prompted us to develop a necrosis model in tissue culture. Live cells obtained from exponentially growing cultures and dead cells obtained from nutritionally depleted plateau-phase cultures were incubated with 99mTc-pyrophosphate, 99mTc-hydroxyethylene diphosphonate (99mTc-HEDP), 67Ga-citrate, and 45Ca-HEDP. For the bone-seeking 99mTc chelates, the ratio of dead to live cell radioactivity was 15--20 after 1 hr of incubation. On the other hand, no preferential binding of 67Ga-citrate was observed, indicating that the mechanism of 67Ga localization in infarcts is different from that of the 99mTc chelates. The death cells accumulated only twice as much 45Ca-HEDP or 45Ca-pyrophosphate as did live cells, possibly due to the carrier effect of calcium in the medium. The receptors for 99mTc chelates could be partially blocked by preincubating the dead cells with microgram amounts of Na-HEDP. Heat-denaturation of live cells also increased the localization of 99mTc-HEDP in the cells, and this uptake was proportional to the duration of heating. Bone-seeking 99mTc chelates could be used for marking dead cells instead of conventional staining methods.", "contents": "Localization of skeletal-imaging 99mTc chelates in dead cells in tissue culture: concise communication. Screening new agents for imaging myocardial infarcts and investigating the mechanism of cellular uptake and subcellular localization of these agents prompted us to develop a necrosis model in tissue culture. Live cells obtained from exponentially growing cultures and dead cells obtained from nutritionally depleted plateau-phase cultures were incubated with 99mTc-pyrophosphate, 99mTc-hydroxyethylene diphosphonate (99mTc-HEDP), 67Ga-citrate, and 45Ca-HEDP. For the bone-seeking 99mTc chelates, the ratio of dead to live cell radioactivity was 15--20 after 1 hr of incubation. On the other hand, no preferential binding of 67Ga-citrate was observed, indicating that the mechanism of 67Ga localization in infarcts is different from that of the 99mTc chelates. The death cells accumulated only twice as much 45Ca-HEDP or 45Ca-pyrophosphate as did live cells, possibly due to the carrier effect of calcium in the medium. The receptors for 99mTc chelates could be partially blocked by preincubating the dead cells with microgram amounts of Na-HEDP. Heat-denaturation of live cells also increased the localization of 99mTc-HEDP in the cells, and this uptake was proportional to the duration of heating. Bone-seeking 99mTc chelates could be used for marking dead cells instead of conventional staining methods.", "PMID": 978263}
{"id": "pubmed23n0032_11622", "title": "Dietary fat level as affecting running performance and other performance-related parameters of rats restricted or non-restricted in food intake.", "content": "The effect of food energy density on certain physical performance characteristics of rats was studied during ad libitum and weight-restricted feeding. After a 16-week treadmill training period, 48 young adult male Long-Evans rats were divided into six groups, receiving 20%, 40% or 70% of energy as dietary fat and fed ad libitum or 40% by weight of average ad libitum intake. They were run to exhaustion once a week. Increased dietary fat level was not correlated with superior running performance at either food intake level, although during restricted feeding the high fat group received 40% more energy than the low fat group. Lower body weight was correlated with increased running performance at both food intake levels. The rate of performance increase with decreasing body weight during food restriction was significantly higher for the low fat than for the high fat group. Water consumption was considerably lower during restricted than during ad libitum feeding. Plasma glucose was lower when the 70% fat diet was fed than when compared to the other fat diets at both levels of food intake, lower from day 8 on of restricted that during ad libitum food intake, and lower on day 15 than on day 8 of the restricted intake.", "contents": "Dietary fat level as affecting running performance and other performance-related parameters of rats restricted or non-restricted in food intake. The effect of food energy density on certain physical performance characteristics of rats was studied during ad libitum and weight-restricted feeding. After a 16-week treadmill training period, 48 young adult male Long-Evans rats were divided into six groups, receiving 20%, 40% or 70% of energy as dietary fat and fed ad libitum or 40% by weight of average ad libitum intake. They were run to exhaustion once a week. Increased dietary fat level was not correlated with superior running performance at either food intake level, although during restricted feeding the high fat group received 40% more energy than the low fat group. Lower body weight was correlated with increased running performance at both food intake levels. The rate of performance increase with decreasing body weight during food restriction was significantly higher for the low fat than for the high fat group. Water consumption was considerably lower during restricted than during ad libitum feeding. Plasma glucose was lower when the 70% fat diet was fed than when compared to the other fat diets at both levels of food intake, lower from day 8 on of restricted that during ad libitum food intake, and lower on day 15 than on day 8 of the restricted intake.", "PMID": 978264}
{"id": "pubmed23n0032_11623", "title": "Influence of fat level and type of carbohydrate on the capacity of pectin in lowering serum and liver lipids of young rats.", "content": "The effect of pectin on lowering cholesterol and fat concentration in tissues of the rat was investigated in relation to the level of fat and the type of carbohydrate. Groups of rats were fed nutritionally complete diets containing equal protein: energy, varied with 5% (low-fat, 2% corn oil plus 3% beff tallow) or 20% (high-fat, 2% corn oil plus 18% beef tallow) fat and cornstarch or sucrose as carbohydrate for 2 weeks. Although there were no interactions, the feeding of pectin reduced the cholesterol level in liver and serum from dietary cholesterol to a greater degree in rats fed the low-fat than in those fed the high-fat diet. This effectiveness also varied with type of carbohydrate. In the low-fat diet, this effectiveness was higher with sucrose than with cornstarch, but in the high-fat diet, it was reversed. The size of perirenal fat pads and the concentrations of total liver lipid and cholesterol were less in rats fed the high-fat pectin diet than in those fed the high-fat diet without pectin, indicating that pectin also had an ability to lower fat absorption. When the level of serum alkaline phosphatase was lowered, pectin was more effective in lowering cholesterol level. This phenomenon, however, seemed due to the type of carbohydrate and fat rather than the cholesterol in the diet. Dietary cholesterol had no effect on the size of perirenal fat pads, but increased liver lipid content greatly in rats fed the high-fat diet. Dietary pectin increased the rate of removal of serum cholesterol, and a similar trend was shown in liver cholesterol which had accumulated from the pre-fed hyper-cholesterol diet.", "contents": "Influence of fat level and type of carbohydrate on the capacity of pectin in lowering serum and liver lipids of young rats. The effect of pectin on lowering cholesterol and fat concentration in tissues of the rat was investigated in relation to the level of fat and the type of carbohydrate. Groups of rats were fed nutritionally complete diets containing equal protein: energy, varied with 5% (low-fat, 2% corn oil plus 3% beff tallow) or 20% (high-fat, 2% corn oil plus 18% beef tallow) fat and cornstarch or sucrose as carbohydrate for 2 weeks. Although there were no interactions, the feeding of pectin reduced the cholesterol level in liver and serum from dietary cholesterol to a greater degree in rats fed the low-fat than in those fed the high-fat diet. This effectiveness also varied with type of carbohydrate. In the low-fat diet, this effectiveness was higher with sucrose than with cornstarch, but in the high-fat diet, it was reversed. The size of perirenal fat pads and the concentrations of total liver lipid and cholesterol were less in rats fed the high-fat pectin diet than in those fed the high-fat diet without pectin, indicating that pectin also had an ability to lower fat absorption. When the level of serum alkaline phosphatase was lowered, pectin was more effective in lowering cholesterol level. This phenomenon, however, seemed due to the type of carbohydrate and fat rather than the cholesterol in the diet. Dietary cholesterol had no effect on the size of perirenal fat pads, but increased liver lipid content greatly in rats fed the high-fat diet. Dietary pectin increased the rate of removal of serum cholesterol, and a similar trend was shown in liver cholesterol which had accumulated from the pre-fed hyper-cholesterol diet.", "PMID": 978265}
{"id": "pubmed23n0032_11624", "title": "Metallothionein and its relationship to the metabolism of dietary zinc in rats.", "content": "Metallothionein (MTN), a low molecular weight cytoplasmic metalloprotein has previously been implicated in the metabolism of Zn. Experiments were conducted with rats to determine the effect of feeding varying levels of dietary Zn for short periods on the increase or decrease in MTN-bound Zn. The results indicated that MTN-Zn in both liver and intestinal mucosal cytosol responded rapidly to an altered dietary Zn level and that serum Zn was directly related to the appearance of MTN. 65Zn absorption also responded rapidly to a change in dietary Zn and was inversely correlated with intestinal mucosal MTN-Zn. Hepatic 65Zn uptake appeared to be directly correlated with liver MTN-Zn. A 24 hour fast increased MTN-Zn in rats fed two different levels of Zn except in those rats that were previously treated with actinomycin D. The antibiotic, which blocks DNA-dependent RNA synthesis, appeared to block Zn uptake from blood to the liver. These findings offer support for the involvement of MTN in zinc metabolism.", "contents": "Metallothionein and its relationship to the metabolism of dietary zinc in rats. Metallothionein (MTN), a low molecular weight cytoplasmic metalloprotein has previously been implicated in the metabolism of Zn. Experiments were conducted with rats to determine the effect of feeding varying levels of dietary Zn for short periods on the increase or decrease in MTN-bound Zn. The results indicated that MTN-Zn in both liver and intestinal mucosal cytosol responded rapidly to an altered dietary Zn level and that serum Zn was directly related to the appearance of MTN. 65Zn absorption also responded rapidly to a change in dietary Zn and was inversely correlated with intestinal mucosal MTN-Zn. Hepatic 65Zn uptake appeared to be directly correlated with liver MTN-Zn. A 24 hour fast increased MTN-Zn in rats fed two different levels of Zn except in those rats that were previously treated with actinomycin D. The antibiotic, which blocks DNA-dependent RNA synthesis, appeared to block Zn uptake from blood to the liver. These findings offer support for the involvement of MTN in zinc metabolism.", "PMID": 978266}
{"id": "pubmed23n0032_11625", "title": "myo-Inositol metabolism during lactation and development in the rat. The prevention of lactation-induced fatty liver by dietary myo-inositol.", "content": "Effects of dietary myo-inositol deprivation were examined during prenatal and postnatal development and during lactation in the rat. The deficient diet contained no detectable myo-inositol while the supplemented diet contained 0.5% (w/w) myo-inositol while the supplemented diet ct contained 0.5% (w/w) myo-inositol at the expense of sucrose. Both diets contained 25% casein, adequate amounts of all known vitamins, choline, and essential fatty acids as well as 0.5% (w/w) phthalylsulfathiazole to depress myo-inositol contribution to the diet by microorganisms. Pregnant rats of the Holtzman strain were fed the respective diets during gestation and lactation, and pups were fed the corresponding diet after weaning until 3 months of age. There were no significant differen-es in body weight between experimental groups. Supplementation of the diet with myo-inositol significanly increased the levels of myo-inositol in plasma, liver, kidney, and intestine of pups at all ages examined, and significantly increased the levels of myo-inositol in the milk and mammary tissue during lactation. During lactation, the myo-inositol deprived dams developed severe fatty livers (31% w/w) characterized by diminished phosphatidyl-inositol (50%) and total phospholipid phosphorus (57%) levels as compared with controls. After weaning, the liver lipid content of the myo-inositol deprived dams returned to normal (4.5%). The data suggest that a possible threshold level of free myo-inositol (approximately 0.15 mumoles/g lipid-free tissue) was required to prevent fatty liver in lactating dams under these dietary conditions. Effects of the deficient diet on fertility were also examined. Based on sperm count and production of offspring, there were no differnences between the experimental and control males. Females of both groups showed equal ability to produce offspring.", "contents": "myo-Inositol metabolism during lactation and development in the rat. The prevention of lactation-induced fatty liver by dietary myo-inositol. Effects of dietary myo-inositol deprivation were examined during prenatal and postnatal development and during lactation in the rat. The deficient diet contained no detectable myo-inositol while the supplemented diet contained 0.5% (w/w) myo-inositol while the supplemented diet ct contained 0.5% (w/w) myo-inositol at the expense of sucrose. Both diets contained 25% casein, adequate amounts of all known vitamins, choline, and essential fatty acids as well as 0.5% (w/w) phthalylsulfathiazole to depress myo-inositol contribution to the diet by microorganisms. Pregnant rats of the Holtzman strain were fed the respective diets during gestation and lactation, and pups were fed the corresponding diet after weaning until 3 months of age. There were no significant differen-es in body weight between experimental groups. Supplementation of the diet with myo-inositol significanly increased the levels of myo-inositol in plasma, liver, kidney, and intestine of pups at all ages examined, and significantly increased the levels of myo-inositol in the milk and mammary tissue during lactation. During lactation, the myo-inositol deprived dams developed severe fatty livers (31% w/w) characterized by diminished phosphatidyl-inositol (50%) and total phospholipid phosphorus (57%) levels as compared with controls. After weaning, the liver lipid content of the myo-inositol deprived dams returned to normal (4.5%). The data suggest that a possible threshold level of free myo-inositol (approximately 0.15 mumoles/g lipid-free tissue) was required to prevent fatty liver in lactating dams under these dietary conditions. Effects of the deficient diet on fertility were also examined. Based on sperm count and production of offspring, there were no differnences between the experimental and control males. Females of both groups showed equal ability to produce offspring.", "PMID": 978267}
{"id": "pubmed23n0032_11626", "title": "Absorption of dietary beta-sitosterol in laying hens and its incorporation into the egg.", "content": "Studies were undertaken to determine the dietary beta-sitosterol absorption in laying hens and its incorporation into the egg. Hens were divided into four groups and fed a commercial low-fat laying diet. Group 1 served as controls; the diet of group 2 was supplemented with 10% corn oil; group 3 with 4% plant sterols (emulsion in carboxymethylcellulose); group 4 with 10% corn oil and 4% plant sterols. The daily beta-sitosterol intake of hens in groups 1, 2, 3 and 4 was 0.036 g 0.095 g, 2 g and 2.56 g, respectively. After consuming the diets for 30 to 40 days, cholesterol and beta-sitosterol contents of the eggs were estimated by gas liquid chromatographic and mass spectrometric methods. Feeding corn oil with and without plant sterols increased cholesterol content of the eggs, while feeding plant sterols alone had no effect on egg cholesterol levels. The beta-sitosterol content of eggs from groups 1 and 2 was similar (60-66 mug/g yolk) and feeding plant sterols increased the beta-sitosterol content to a very small extent (82-90 mug/g yolk). Studies on the dietary beta-sitosterol and cholesterol absorption indicated that there was very little absorption of beta-sitosterol. In control hens, beta-sitosterol absorption was about 7% compared to 81% cholesterol absorption. Plant sterols feeding did not affect the beta-sitosterol absorption but reduced cholesterol absorption by 40%. Feeding corn oil alone did not affect cholesterol absorption. Failure to detect any significant amount of beta-sitosterol in these hens. At the peak radioactivity deposition in the eggs, only 2% of the absorbed beta-sitosterol radioactivity was found in the eggs of group 4 compared to 5% found in group 1, while cholesterol radiioactivity was nearly 4 to 5 times in both groups. These data suggested that laying hens synthesized specific lipoproteins for deposition in yolk.", "contents": "Absorption of dietary beta-sitosterol in laying hens and its incorporation into the egg. Studies were undertaken to determine the dietary beta-sitosterol absorption in laying hens and its incorporation into the egg. Hens were divided into four groups and fed a commercial low-fat laying diet. Group 1 served as controls; the diet of group 2 was supplemented with 10% corn oil; group 3 with 4% plant sterols (emulsion in carboxymethylcellulose); group 4 with 10% corn oil and 4% plant sterols. The daily beta-sitosterol intake of hens in groups 1, 2, 3 and 4 was 0.036 g 0.095 g, 2 g and 2.56 g, respectively. After consuming the diets for 30 to 40 days, cholesterol and beta-sitosterol contents of the eggs were estimated by gas liquid chromatographic and mass spectrometric methods. Feeding corn oil with and without plant sterols increased cholesterol content of the eggs, while feeding plant sterols alone had no effect on egg cholesterol levels. The beta-sitosterol content of eggs from groups 1 and 2 was similar (60-66 mug/g yolk) and feeding plant sterols increased the beta-sitosterol content to a very small extent (82-90 mug/g yolk). Studies on the dietary beta-sitosterol and cholesterol absorption indicated that there was very little absorption of beta-sitosterol. In control hens, beta-sitosterol absorption was about 7% compared to 81% cholesterol absorption. Plant sterols feeding did not affect the beta-sitosterol absorption but reduced cholesterol absorption by 40%. Feeding corn oil alone did not affect cholesterol absorption. Failure to detect any significant amount of beta-sitosterol in these hens. At the peak radioactivity deposition in the eggs, only 2% of the absorbed beta-sitosterol radioactivity was found in the eggs of group 4 compared to 5% found in group 1, while cholesterol radiioactivity was nearly 4 to 5 times in both groups. These data suggested that laying hens synthesized specific lipoproteins for deposition in yolk.", "PMID": 978268}
{"id": "pubmed23n0032_11627", "title": "Characteristic inhibition of in vitro swelling of purified rat liver mitochondria induced by Ca2+ or thyroxine by feeding rats a low casein diet.", "content": "It has been shown previously by the author that the structure of rat liver mitochondria is changed by a low casein diet. In the present experiments, using the free fatty acid producing substances Ca2+ and thyroxine, and the free fatty acid sodium oleate, the influence of a 4% casein diet on the in vitro swelling process of purified rat liver mitochondria was investigated by following the decrease in optical density at 520 nm after addition of one of the swelling agents. Swelling due to hypotonicity was also investigated. Rats were fed the test diets for 80 to 100 days. Mitochondria were purified by washing three times; after each wash the isotonic suspension was centrifuged at 3,000 X g for 6 minutes. The results show that the in vitro swelling of mitochondria induced by Ca2+ or thyroxine was inhibited greatly in the early period of swelling in rats fed a low casein diet. By comparison to this, there was not a notable effect of a low casein diet on the rate of swelling induced by sodium oleate. But in the case of sodium oleate, the values of optical density were always higher in rats fed a low casein diet, and the differences between two groups were always significant. Swelling due to hypotonicity was not affected at all by a low casein diet.", "contents": "Characteristic inhibition of in vitro swelling of purified rat liver mitochondria induced by Ca2+ or thyroxine by feeding rats a low casein diet. It has been shown previously by the author that the structure of rat liver mitochondria is changed by a low casein diet. In the present experiments, using the free fatty acid producing substances Ca2+ and thyroxine, and the free fatty acid sodium oleate, the influence of a 4% casein diet on the in vitro swelling process of purified rat liver mitochondria was investigated by following the decrease in optical density at 520 nm after addition of one of the swelling agents. Swelling due to hypotonicity was also investigated. Rats were fed the test diets for 80 to 100 days. Mitochondria were purified by washing three times; after each wash the isotonic suspension was centrifuged at 3,000 X g for 6 minutes. The results show that the in vitro swelling of mitochondria induced by Ca2+ or thyroxine was inhibited greatly in the early period of swelling in rats fed a low casein diet. By comparison to this, there was not a notable effect of a low casein diet on the rate of swelling induced by sodium oleate. But in the case of sodium oleate, the values of optical density were always higher in rats fed a low casein diet, and the differences between two groups were always significant. Swelling due to hypotonicity was not affected at all by a low casein diet.", "PMID": 978269}
{"id": "pubmed23n0032_11628", "title": "The biotin requirement of rats fed 20% egg white.", "content": "The biotin requirement of rats fed a diet based upon 62.4% sucrose, 20.0% egg white and 10.0% corn oil was determined using growth for 60 days as the cirterion. Maximal growth and maximal concentration of cholesterol in plasma occurred when the diet contained 2.00 mg biotin/kg. Growth depression was noted in one of two experiments in which 3.00 mg of biotin was present per kg of diet.", "contents": "The biotin requirement of rats fed 20% egg white. The biotin requirement of rats fed a diet based upon 62.4% sucrose, 20.0% egg white and 10.0% corn oil was determined using growth for 60 days as the cirterion. Maximal growth and maximal concentration of cholesterol in plasma occurred when the diet contained 2.00 mg biotin/kg. Growth depression was noted in one of two experiments in which 3.00 mg of biotin was present per kg of diet.", "PMID": 978270}
{"id": "pubmed23n0032_11629", "title": "Maternal protein malnutrition during gestation alone and its effects on plasma insulin levels of the pregnant pig, its fetuses and the developing offspring.", "content": "The effects of restricting protein intake on plasma insulin were studied in pregnant pigs, fetuses and the developing offspring. Pregnant pigs were fed diets containing 18%, 3% or 0.5% protein throughout the gestation period. At 10, 13 and 15 weeks of gestation, fetuses were removed from the uterus after bleeding the dam. Plasma samples were used for insulin determination by a radioimmunoassay procedure. At week 15 of gestation, plasma insulin levels were significantly higher in pregnant pigs fed 18% protein and also in their fetuses than in the other two groups. There was a high correlation between fetal insulin level and fetal growth rate (r = 0.84). Two-day-pld pigs from another set of pregnant pigs fed the diet containing 18%, 3%, or 0.5% protein during gestation were cross-fostered to control nursing dams and weaned at 4 weeks of age to a standard diet. Plasma smaples obtained at regular intervals were used for insulin determination. Offspring of pigs fed 0.5% protein during gestation had consistently low insulin levels in postnatal life in spite of cross-fostering and standard feeding after weaning. It appears that one of the stimuli which control maternal insulin secretion and placenta transfer is the maternal protein intake while severe maternal protein restriction might contribute to the low levels of insulin in the progeny during postnatal life.", "contents": "Maternal protein malnutrition during gestation alone and its effects on plasma insulin levels of the pregnant pig, its fetuses and the developing offspring. The effects of restricting protein intake on plasma insulin were studied in pregnant pigs, fetuses and the developing offspring. Pregnant pigs were fed diets containing 18%, 3% or 0.5% protein throughout the gestation period. At 10, 13 and 15 weeks of gestation, fetuses were removed from the uterus after bleeding the dam. Plasma samples were used for insulin determination by a radioimmunoassay procedure. At week 15 of gestation, plasma insulin levels were significantly higher in pregnant pigs fed 18% protein and also in their fetuses than in the other two groups. There was a high correlation between fetal insulin level and fetal growth rate (r = 0.84). Two-day-pld pigs from another set of pregnant pigs fed the diet containing 18%, 3%, or 0.5% protein during gestation were cross-fostered to control nursing dams and weaned at 4 weeks of age to a standard diet. Plasma smaples obtained at regular intervals were used for insulin determination. Offspring of pigs fed 0.5% protein during gestation had consistently low insulin levels in postnatal life in spite of cross-fostering and standard feeding after weaning. It appears that one of the stimuli which control maternal insulin secretion and placenta transfer is the maternal protein intake while severe maternal protein restriction might contribute to the low levels of insulin in the progeny during postnatal life.", "PMID": 978271}
{"id": "pubmed23n0032_11630", "title": "Effect of glycerol on lipogenic enzyme activities and on fatty acid synthesis in the rat and chicken.", "content": "The influence of glycerol on the rates of fatty acid snythesis in liver slices from rats and chickens in pieces of adipose tissue from rats was first studied. Then the effect of dietary glycerol on lipid metabolism in rats and cheickens was examined. Media containing 3 or 10 mM glycerol depressed the rate of glucose conversion to fatty acids in rat liver slices. However, media containing up to 25 mM glycerol did not influence the rate of fatty acid synthesis in chick liver slices. The inhibitory action of glycerol in rat liver slices might occur at the level of glucose (or glycogen) conversion to pyruvate because glycerol did not inhibit pyruvate or acetate conversion to fatty acids. Rats and chickens were fed glycerol containing diets for either 3 days or 3 weeks. Feeding diets containing 20.5 parts glycerol (22% of dietary energy) to rats or chickens did not influence the growth rate of the animals. However, substitution of 42.2 parts glycerol (43% of dietary energy) for glucose in the diet significantly depressed food intake and growth rate in both rats and chickens. The activities of citrate cleavage enzyme, fatty acid synthetase and malic enzyme in livers of rats fed the glycerol-containing diets were dramatically increased. However, this stimulation of enzyme activity occurred without a concomitant increase in the in vivo rate of fatty acid synthesis in the rat liver. In the chicken, unlike the rat, dietary glycerol did not stimulate but instead decreased hepatic malic enzyme and fatty acid synthetase activities. No significant differences in adipose tissue lipogenic enzyme activities or in the rates of fatty acid synthesis were observed in rats fed glycerol-containing diets. The lipogenic response to glycerol feeding depends on the species as well as the organ.", "contents": "Effect of glycerol on lipogenic enzyme activities and on fatty acid synthesis in the rat and chicken. The influence of glycerol on the rates of fatty acid snythesis in liver slices from rats and chickens in pieces of adipose tissue from rats was first studied. Then the effect of dietary glycerol on lipid metabolism in rats and cheickens was examined. Media containing 3 or 10 mM glycerol depressed the rate of glucose conversion to fatty acids in rat liver slices. However, media containing up to 25 mM glycerol did not influence the rate of fatty acid synthesis in chick liver slices. The inhibitory action of glycerol in rat liver slices might occur at the level of glucose (or glycogen) conversion to pyruvate because glycerol did not inhibit pyruvate or acetate conversion to fatty acids. Rats and chickens were fed glycerol containing diets for either 3 days or 3 weeks. Feeding diets containing 20.5 parts glycerol (22% of dietary energy) to rats or chickens did not influence the growth rate of the animals. However, substitution of 42.2 parts glycerol (43% of dietary energy) for glucose in the diet significantly depressed food intake and growth rate in both rats and chickens. The activities of citrate cleavage enzyme, fatty acid synthetase and malic enzyme in livers of rats fed the glycerol-containing diets were dramatically increased. However, this stimulation of enzyme activity occurred without a concomitant increase in the in vivo rate of fatty acid synthesis in the rat liver. In the chicken, unlike the rat, dietary glycerol did not stimulate but instead decreased hepatic malic enzyme and fatty acid synthetase activities. No significant differences in adipose tissue lipogenic enzyme activities or in the rates of fatty acid synthesis were observed in rats fed glycerol-containing diets. The lipogenic response to glycerol feeding depends on the species as well as the organ.", "PMID": 978272}
{"id": "pubmed23n0032_11631", "title": "Leukocyte transketolase activity as an indicator of thiamin nutriture in rats.", "content": "The purpose of this study was to evaluate the possibility of using leukocyte transketolase activity (TKA) as an index of thiamin status. It was shown previously that hepatic TKA in rats is altered by changes in the type and amounts of carbohydrate, protein, and fat in the diet. It was, therefore, essential to determine whether leukocyte TKA in rats is affected by dietary factors other than thiamin intake. Hepatic TKA and leukocyte TKA both varied with dietary thiamin intake; maximum activity was attained when the diet contained 3 to 4 mg thiamin/kg diet. Administration of oxythiamin resulted in decreases in TKA in both liver and leukocytes. The changes in leukocyte and hepatic TKA induced by varying degrees of thiamin deficiency were closely correlated. The responses of thiamin content were also closely correlated in liver and leukocytes. However, only hepatic TKA was altered by increasing the fat or protein content of the diet or by substituting fructose for glucose. None of the dietary manipulations studied except the production of thiamin deficiency had any effect on leukocyte TKA. We conclude from our results that leukocyte TKA is a sensitive and specific indicator of thiamin nutriture in rats.", "contents": "Leukocyte transketolase activity as an indicator of thiamin nutriture in rats. The purpose of this study was to evaluate the possibility of using leukocyte transketolase activity (TKA) as an index of thiamin status. It was shown previously that hepatic TKA in rats is altered by changes in the type and amounts of carbohydrate, protein, and fat in the diet. It was, therefore, essential to determine whether leukocyte TKA in rats is affected by dietary factors other than thiamin intake. Hepatic TKA and leukocyte TKA both varied with dietary thiamin intake; maximum activity was attained when the diet contained 3 to 4 mg thiamin/kg diet. Administration of oxythiamin resulted in decreases in TKA in both liver and leukocytes. The changes in leukocyte and hepatic TKA induced by varying degrees of thiamin deficiency were closely correlated. The responses of thiamin content were also closely correlated in liver and leukocytes. However, only hepatic TKA was altered by increasing the fat or protein content of the diet or by substituting fructose for glucose. None of the dietary manipulations studied except the production of thiamin deficiency had any effect on leukocyte TKA. We conclude from our results that leukocyte TKA is a sensitive and specific indicator of thiamin nutriture in rats.", "PMID": 978273}
{"id": "pubmed23n0032_11632", "title": "The role of thiamine on excitable membrane of crayfish giant axon.", "content": "Electrophysiological and biochemical studies were performed to determine the role of thiamine in the excitable membrane of the crayfish giant axons, as it has been suggested that thiamine plays a role in the excitability of the membrane which is unrelated to the metabolic process. Thiamine (5mM) significantly increased the rising rate of the action potential without affecting the resting membrane potential and threshold potential. A recovery of the enhancement was evidenced by a wash of the axon with physiological solution. Pyrithiamine (10 mM) reduced the rising rate of the action potential without affecting the membrane and threshold potential. The reduction of dV/dt by pyrithiamine remained unchanged after a wash of the axon with physiological solution, while dV/dt increased after thiamine treatment. The amount of thiamine found in the crayfish axons was comparable to that observed in the rat sciatic nerve. In addition, pyrithiamine reduced the thiamine content in axons and protein binding thiamine of the axons. It is thus concluded that thiamine in the excitable membrane of crayfish axons plays a significant role in production of the action potential and is essential for maintaining the membrane excitability of crayfish axons.", "contents": "The role of thiamine on excitable membrane of crayfish giant axon. Electrophysiological and biochemical studies were performed to determine the role of thiamine in the excitable membrane of the crayfish giant axons, as it has been suggested that thiamine plays a role in the excitability of the membrane which is unrelated to the metabolic process. Thiamine (5mM) significantly increased the rising rate of the action potential without affecting the resting membrane potential and threshold potential. A recovery of the enhancement was evidenced by a wash of the axon with physiological solution. Pyrithiamine (10 mM) reduced the rising rate of the action potential without affecting the membrane and threshold potential. The reduction of dV/dt by pyrithiamine remained unchanged after a wash of the axon with physiological solution, while dV/dt increased after thiamine treatment. The amount of thiamine found in the crayfish axons was comparable to that observed in the rat sciatic nerve. In addition, pyrithiamine reduced the thiamine content in axons and protein binding thiamine of the axons. It is thus concluded that thiamine in the excitable membrane of crayfish axons plays a significant role in production of the action potential and is essential for maintaining the membrane excitability of crayfish axons.", "PMID": 978275}
{"id": "pubmed23n0032_11633", "title": "Absorption, utilization and clinical effectiveness of allithiamines compared to water-soluble thiamines.", "content": "Oral administration of lipid-soluble allithiamines [thiamine propyl disulfide (TPD) and thiamine tetrahydrofurfuryl disulfide (TTHF)] rapidly increased thiamine activity in whole blood, red blood cells, cerebrospinal fluid, and urine in normal and thiamine-deficient subjects. These thiamine congeners also restored red blood cell transketolase to normal in alcoholics with thiamine deficiency. Such repletion equaled that produced by parenteral, water-soluble thiamine hydrochloride (THCl) or thiamine pyrophosphate (TPP). Oral administration of water-soluble thiamines (THCl, TPP) neither elevated thiamine activity in biological fluids nor restored transketolase activity to normal in alcoholics with thiamine deficiency presumably due to their rate-limited intestinal transport. Oral administration of TPD eliminated lateral rectus palsy in patients with Wernicke's encephalopathy. Orally administered allithiamine vitamers are therefore recommended for prophylaxis and treatment of thiamine deficits because while having essentially the same biological properties as parenterally administered water-soluble thiamines they have not produced any untoward effects after long-term administration and are far more efficiently utilized.", "contents": "Absorption, utilization and clinical effectiveness of allithiamines compared to water-soluble thiamines. Oral administration of lipid-soluble allithiamines [thiamine propyl disulfide (TPD) and thiamine tetrahydrofurfuryl disulfide (TTHF)] rapidly increased thiamine activity in whole blood, red blood cells, cerebrospinal fluid, and urine in normal and thiamine-deficient subjects. These thiamine congeners also restored red blood cell transketolase to normal in alcoholics with thiamine deficiency. Such repletion equaled that produced by parenteral, water-soluble thiamine hydrochloride (THCl) or thiamine pyrophosphate (TPP). Oral administration of water-soluble thiamines (THCl, TPP) neither elevated thiamine activity in biological fluids nor restored transketolase activity to normal in alcoholics with thiamine deficiency presumably due to their rate-limited intestinal transport. Oral administration of TPD eliminated lateral rectus palsy in patients with Wernicke's encephalopathy. Orally administered allithiamine vitamers are therefore recommended for prophylaxis and treatment of thiamine deficits because while having essentially the same biological properties as parenterally administered water-soluble thiamines they have not produced any untoward effects after long-term administration and are far more efficiently utilized.", "PMID": 978282}
{"id": "pubmed23n0032_11634", "title": "Reactivation mechanisms of thiamine with thermostable factors.", "content": "It was observed, in vitro, that the water extract of the fermented-tea customarily chewed by Thai people has a similar thermostable thiamine-inactivating factor to that found in the water extract of fern. It was also observed that the percentage of thiamine disulfide formed from thiamine with some flavones, catechol, pyrogallol, caffeic acid, dihydroxyphenylalanine, and hemin is greater at pH 7.5 than at pH 7.0. With some flavonoids, such as quercetin, rutin, and 6,7,4'-trihydroxyisoflavone, and pyrogallol, hemin, catechol and caffeic acid at pH 7.5, around 30-100% of thiamine is changed into thiamine disulfide. Water extract of shiitake, okra, coffee, black tea and fukinoto have only weak activities of thermostable thiamine-inactivating factors as a large percentage of thiamine disulfide is formed from thiamine even at pH 7.0. 2-Methyl-4-amino-5-aminomethylpyrimidine was isolated from the reaction mixture of 1 g thiamine with 20 mg catechol (1:0.5 mole) at pH 7.0, 45 degrees C, and identified with the synthesized pyrimidine.", "contents": "Reactivation mechanisms of thiamine with thermostable factors. It was observed, in vitro, that the water extract of the fermented-tea customarily chewed by Thai people has a similar thermostable thiamine-inactivating factor to that found in the water extract of fern. It was also observed that the percentage of thiamine disulfide formed from thiamine with some flavones, catechol, pyrogallol, caffeic acid, dihydroxyphenylalanine, and hemin is greater at pH 7.5 than at pH 7.0. With some flavonoids, such as quercetin, rutin, and 6,7,4'-trihydroxyisoflavone, and pyrogallol, hemin, catechol and caffeic acid at pH 7.5, around 30-100% of thiamine is changed into thiamine disulfide. Water extract of shiitake, okra, coffee, black tea and fukinoto have only weak activities of thermostable thiamine-inactivating factors as a large percentage of thiamine disulfide is formed from thiamine even at pH 7.0. 2-Methyl-4-amino-5-aminomethylpyrimidine was isolated from the reaction mixture of 1 g thiamine with 20 mg catechol (1:0.5 mole) at pH 7.0, 45 degrees C, and identified with the synthesized pyrimidine.", "PMID": 978283}
{"id": "pubmed23n0032_11635", "title": "Effect of field size on red--green color mixture equations.", "content": "Red-green color mixture equations were measured in 10 color-normal observers for fields of view varying from 30' to 10 degrees. The G/R mixture decreases continuously as field size is increased. The data are consistent with the interpretation that the cone visual photopigments decrease exponentially in effective optical density as the field size is increased.", "contents": "Effect of field size on red--green color mixture equations. Red-green color mixture equations were measured in 10 color-normal observers for fields of view varying from 30' to 10 degrees. The G/R mixture decreases continuously as field size is increased. The data are consistent with the interpretation that the cone visual photopigments decrease exponentially in effective optical density as the field size is increased.", "PMID": 978285}
{"id": "pubmed23n0032_11636", "title": "Luminance and opponent-color contributions to visual detection and adaptation and to temporal and spatial integration.", "content": "We show how the processes of visual detection and of temporal and spatial summation may be analyzed in terms of parallel luminance (achromatic) and opponent-color systems; a test flash is detected if it exceeds the threshold of either system. The spectral sensitivity of the luminance system may be determined by a flicker method, and has a single broad peak near 555 nm; the spectral sensitivity of the opponent-color system corresponds to the color recognition threshold, and has three peaks at about 440, 530, and 600 nm (on a white background). The temporal and spatial integration of the opponent-color system are generally greater than for the luminance system; further, a white background selectively depresses the sensitivity of the luminance system relative to the opponent-color system. Thus relatively large (1 degree) and long (200 msec) spectral test flashes on a white background are detected by the opponent-color system except near 570 nm; the contribution of the luminance system becomes more prominent if the size or duration of the test flash is reduced, or if the white background is extinguished. The present analysis is discussed in relation to Stiles' model of independent eta mechanisms.", "contents": "Luminance and opponent-color contributions to visual detection and adaptation and to temporal and spatial integration. We show how the processes of visual detection and of temporal and spatial summation may be analyzed in terms of parallel luminance (achromatic) and opponent-color systems; a test flash is detected if it exceeds the threshold of either system. The spectral sensitivity of the luminance system may be determined by a flicker method, and has a single broad peak near 555 nm; the spectral sensitivity of the opponent-color system corresponds to the color recognition threshold, and has three peaks at about 440, 530, and 600 nm (on a white background). The temporal and spatial integration of the opponent-color system are generally greater than for the luminance system; further, a white background selectively depresses the sensitivity of the luminance system relative to the opponent-color system. Thus relatively large (1 degree) and long (200 msec) spectral test flashes on a white background are detected by the opponent-color system except near 570 nm; the contribution of the luminance system becomes more prominent if the size or duration of the test flash is reduced, or if the white background is extinguished. The present analysis is discussed in relation to Stiles' model of independent eta mechanisms.", "PMID": 978286}
{"id": "pubmed23n0032_11637", "title": "Cryoimmunoglobulin IgGk with microtubular ultrastructure associated with pyoderma gangrenosum.", "content": "Recurrent gangrenous leg ulcers were associated with a monoclonal IgGk cryoglobulinaemia, though without overt myeloma. Cryoprecipitation of the serum in vitro gave a reversible gel with microtubular structure, built from the monomer and with a high degree of order. The same structure was found distending the superficial vessels of the ulcer biopsy, as well as extravascularly. There was no vasculitis, and luminal obstruction appeared responsible for the ulceration. Microtubular structures occur in nature among diverse proteins and viruses. The protein studied here is similar to a reported instance of myeloma cryo-IgGk.", "contents": "Cryoimmunoglobulin IgGk with microtubular ultrastructure associated with pyoderma gangrenosum. Recurrent gangrenous leg ulcers were associated with a monoclonal IgGk cryoglobulinaemia, though without overt myeloma. Cryoprecipitation of the serum in vitro gave a reversible gel with microtubular structure, built from the monomer and with a high degree of order. The same structure was found distending the superficial vessels of the ulcer biopsy, as well as extravascularly. There was no vasculitis, and luminal obstruction appeared responsible for the ulceration. Microtubular structures occur in nature among diverse proteins and viruses. The protein studied here is similar to a reported instance of myeloma cryo-IgGk.", "PMID": 978311}
{"id": "pubmed23n0032_11638", "title": "Cardiac biopsy in skeletal myopathy: report of a case with myocardial mitochondrial abnormalities.", "content": "Transvenous right ventricular endomyocardial biopsy in an 11-yr-old boy with a proximal skeletal myopathy and a cardiomyopathy, has shown a major increase in mitochondrial size and numbers on electron-microscopic morphometry, with formation of unique ring-shaped mitochondria in cardiac muscle cells. The significance of this finding and the use of cardiac biopsy in children are discussed.", "contents": "Cardiac biopsy in skeletal myopathy: report of a case with myocardial mitochondrial abnormalities. Transvenous right ventricular endomyocardial biopsy in an 11-yr-old boy with a proximal skeletal myopathy and a cardiomyopathy, has shown a major increase in mitochondrial size and numbers on electron-microscopic morphometry, with formation of unique ring-shaped mitochondria in cardiac muscle cells. The significance of this finding and the use of cardiac biopsy in children are discussed.", "PMID": 978312}
{"id": "pubmed23n0032_11639", "title": "Aspirin damage in the rat kidney in the intact animal and after unilateral nephrectomy.", "content": "Aspirin caused epithelial hyperplasia, papillary necrosis with calcification and cortical changes in the rat kidney. After unilateral nephrectomy the lesions appeared more rapidly and were more severe. In the electron microscope the earliest changes were observed in the interstitial cells.", "contents": "Aspirin damage in the rat kidney in the intact animal and after unilateral nephrectomy. Aspirin caused epithelial hyperplasia, papillary necrosis with calcification and cortical changes in the rat kidney. After unilateral nephrectomy the lesions appeared more rapidly and were more severe. In the electron microscope the earliest changes were observed in the interstitial cells.", "PMID": 978313}
{"id": "pubmed23n0032_11640", "title": "Inflammatory cells in duct-ligated salivary glands of the cat: a histochemical study.", "content": "Inflammatory cells were studied in submandibular, sublingual and parotid glands of cat following ductal ligation. Macrophages were seen within the parenchyma and associated with extravasated mucin, and were possibly of importance in removing secretory material. Neutrophils were seen interstitially and within the parenchyma. Eosinophils were involved in an initial inflammatory response in interstitial tissue. Fibrous connective tissue was more apparent.", "contents": "Inflammatory cells in duct-ligated salivary glands of the cat: a histochemical study. Inflammatory cells were studied in submandibular, sublingual and parotid glands of cat following ductal ligation. Macrophages were seen within the parenchyma and associated with extravasated mucin, and were possibly of importance in removing secretory material. Neutrophils were seen interstitially and within the parenchyma. Eosinophils were involved in an initial inflammatory response in interstitial tissue. Fibrous connective tissue was more apparent.", "PMID": 978314}
{"id": "pubmed23n0032_11641", "title": "Histologic study of biliary fibrous remnants in 48 cases of extrahepatic biliary atresia: correlation with postoperative bile flow restoration.", "content": "The fibrous remnants of bile ducts that were removed at surgery for extrahepatic biliary atresia were studied by serial section. Forty-eight specimens were classified into three types on the basis of histopathologic changes in the proximal portions of the speciments from the vicinity of the porta hepatis: (1) connective tissue without glands or other epithelial structures (15 cases); (2) connective tissue containing glands lined with cuboidal epithelium (15 cases); (3) connective tissue containing central biliary ducts and peripheral glands (18 cases). Histopathologic changes in sections from distal portions of the specimens did not conform to this classification. A precise correlation of prognostic significance could not be established between these histologic data and postoperative restoration of bile flow, although failure to restore flow was encountered most frequently in cases without demonstrable ducts or glands (TYPE 1).", "contents": "Histologic study of biliary fibrous remnants in 48 cases of extrahepatic biliary atresia: correlation with postoperative bile flow restoration. The fibrous remnants of bile ducts that were removed at surgery for extrahepatic biliary atresia were studied by serial section. Forty-eight specimens were classified into three types on the basis of histopathologic changes in the proximal portions of the speciments from the vicinity of the porta hepatis: (1) connective tissue without glands or other epithelial structures (15 cases); (2) connective tissue containing glands lined with cuboidal epithelium (15 cases); (3) connective tissue containing central biliary ducts and peripheral glands (18 cases). Histopathologic changes in sections from distal portions of the specimens did not conform to this classification. A precise correlation of prognostic significance could not be established between these histologic data and postoperative restoration of bile flow, although failure to restore flow was encountered most frequently in cases without demonstrable ducts or glands (TYPE 1).", "PMID": 978315}
{"id": "pubmed23n0032_11642", "title": "Functioning thyroid masses in childhood and adolescence. Clinical, surgical, and pathologic correlations.", "content": "Six girls, aged 5 to 15 years, presented with thyroid masses in otherwise nonpalpable thyroid glands and with normal serum thyroxine levels. Scintiscanning before and after TSH stimulation confirmed the presence of autonomous nodules in the four adolescents, of whom two had elevated T3 levels. Surgical exploration revealed adenomatous thyroid hyperplasia in three of the girls and papillary adenocarcinoma in the fourth. Scans in the other two girls revealed absence of the left lobe. One of them proved to have agenesis of the left lobe with enlargement of the right lobe because of lymphocytic thyroiditis. The other girl had an ectopic thyroid with chronic inflammation. A thorough diagnostic evaluation of single or multiple functioning thyroid masses in children and adolescents is essential in establishing the correct diagnosis. The possibility that carcinoma can occur in autonomous nodules as well as in hemiagenesis and ectopic thyroid tissue is discussed. An approach to the management of functioning thyroid masses in the pediatric age group is proposed.", "contents": "Functioning thyroid masses in childhood and adolescence. Clinical, surgical, and pathologic correlations. Six girls, aged 5 to 15 years, presented with thyroid masses in otherwise nonpalpable thyroid glands and with normal serum thyroxine levels. Scintiscanning before and after TSH stimulation confirmed the presence of autonomous nodules in the four adolescents, of whom two had elevated T3 levels. Surgical exploration revealed adenomatous thyroid hyperplasia in three of the girls and papillary adenocarcinoma in the fourth. Scans in the other two girls revealed absence of the left lobe. One of them proved to have agenesis of the left lobe with enlargement of the right lobe because of lymphocytic thyroiditis. The other girl had an ectopic thyroid with chronic inflammation. A thorough diagnostic evaluation of single or multiple functioning thyroid masses in children and adolescents is essential in establishing the correct diagnosis. The possibility that carcinoma can occur in autonomous nodules as well as in hemiagenesis and ectopic thyroid tissue is discussed. An approach to the management of functioning thyroid masses in the pediatric age group is proposed.", "PMID": 978316}
{"id": "pubmed23n0032_11643", "title": "Circulating bound and free estradiol and estrone during normal growth and development and in premature thelarche and isosexual precocity.", "content": "Serum concentrations of unconjugated estrone, estradiol, and free estradiol, were determined in normal neonates, prepubertal children, adolescents, and adults. The values were compared with those obtained in children with premature thelarche and female sexual precocity. Unconjugated E1 and E2 fell rapidly, and the percentage of FE2 more gradually during the neonatal period and remained low prepubertally. During adolescence girls had greater increases in E1 and E2 while the percentage of FE2 was higher in boys. In premature thelarche  only the FE2 was significantly increased. In sexual precocity E1, E2, and FE2 were elevated. Reference standards are provided in Tables I and II for use in the diagnosis of conditions with under- or overproduction of estrogens during growth and development.", "contents": "Circulating bound and free estradiol and estrone during normal growth and development and in premature thelarche and isosexual precocity. Serum concentrations of unconjugated estrone, estradiol, and free estradiol, were determined in normal neonates, prepubertal children, adolescents, and adults. The values were compared with those obtained in children with premature thelarche and female sexual precocity. Unconjugated E1 and E2 fell rapidly, and the percentage of FE2 more gradually during the neonatal period and remained low prepubertally. During adolescence girls had greater increases in E1 and E2 while the percentage of FE2 was higher in boys. In premature thelarche  only the FE2 was significantly increased. In sexual precocity E1, E2, and FE2 were elevated. Reference standards are provided in Tables I and II for use in the diagnosis of conditions with under- or overproduction of estrogens during growth and development.", "PMID": 978317}
{"id": "pubmed23n0032_11644", "title": "Antibody deficiency and alopecia.", "content": "Three patients are described with antibody deficiency, recurrent infections, and alopecia. One patient had congenital agammaglobulinemia; the other two patients, a brother and sister, had an incomplete antibody deficiency syndrome. The loss of hair in each patient was total; the history was typical of alopecia areata. The association of alopecia and antibody deficiency has not previously been described to our knowledge in children.", "contents": "Antibody deficiency and alopecia. Three patients are described with antibody deficiency, recurrent infections, and alopecia. One patient had congenital agammaglobulinemia; the other two patients, a brother and sister, had an incomplete antibody deficiency syndrome. The loss of hair in each patient was total; the history was typical of alopecia areata. The association of alopecia and antibody deficiency has not previously been described to our knowledge in children.", "PMID": 978318}
{"id": "pubmed23n0032_11645", "title": "Deficiency of adenosine deaminase not associated with severe combined immunodeficiency.", "content": "The 12-year-old Kung (\"\"Bushman'') boy from South West Africa who has marked deficiency of red cell adenosine deaminase has been found to have 2 to 3% of enzyme activity in red blood cells, 10 to 12% in leukocytes, and 10 to 30% in cultured fibroblasts. The enzyme has ADA 1 electrophoretic mobility: SV40 transformation of cultured fibroblasts caused a decrease of \"\"tissue ADA'' and an increase in \"\"red cell ADA'' isozymes. A battery of investigations revealed that the child has normal humoral and cellular immunity. A family study showed that a sibling had the same level of red cell ADA and the parents had intermediate levels. Studies of the Kung population from which the child comes have shown that the allele responsible for the condition, and which we designate ADA8, is polymorphic.", "contents": "Deficiency of adenosine deaminase not associated with severe combined immunodeficiency. The 12-year-old Kung (\"\"Bushman'') boy from South West Africa who has marked deficiency of red cell adenosine deaminase has been found to have 2 to 3% of enzyme activity in red blood cells, 10 to 12% in leukocytes, and 10 to 30% in cultured fibroblasts. The enzyme has ADA 1 electrophoretic mobility: SV40 transformation of cultured fibroblasts caused a decrease of \"\"tissue ADA'' and an increase in \"\"red cell ADA'' isozymes. A battery of investigations revealed that the child has normal humoral and cellular immunity. A family study showed that a sibling had the same level of red cell ADA and the parents had intermediate levels. Studies of the Kung population from which the child comes have shown that the allele responsible for the condition, and which we designate ADA8, is polymorphic.", "PMID": 978319}
{"id": "pubmed23n0032_11646", "title": "The chondro-osseous dysplasia of adenosine deaminase deficiency with severe combined immunodeficiency.", "content": "Three children, two of them siblings, with severe combined immunodeficiency and adenosine deaminase deficiency died within the first six months of life from the complications of acute bacterial infections. Subtle radiographic abnormalities were seen at the costochondral junctions, at the apophysis of the iliac bones, and in the vertebral bodies. At autopsy, the thymus showed evidence of early differentiation and, in one instance, aborted Hassall's corpuscles. Histologic study of the bone disclosed lack of organized cartilage columnar formation, large lacuni containing hypertrophied cells, and lack of trabecular formation with uninterrupted areas of calcified cartilage. These changes are distinctly different from those observed in the metaphyseal chondrodysplasias or in other chondrodystrophies.", "contents": "The chondro-osseous dysplasia of adenosine deaminase deficiency with severe combined immunodeficiency. Three children, two of them siblings, with severe combined immunodeficiency and adenosine deaminase deficiency died within the first six months of life from the complications of acute bacterial infections. Subtle radiographic abnormalities were seen at the costochondral junctions, at the apophysis of the iliac bones, and in the vertebral bodies. At autopsy, the thymus showed evidence of early differentiation and, in one instance, aborted Hassall's corpuscles. Histologic study of the bone disclosed lack of organized cartilage columnar formation, large lacuni containing hypertrophied cells, and lack of trabecular formation with uninterrupted areas of calcified cartilage. These changes are distinctly different from those observed in the metaphyseal chondrodysplasias or in other chondrodystrophies.", "PMID": 978320}
{"id": "pubmed23n0032_11647", "title": "Infection of the urinary tract after anti-reflux surgery.", "content": "Two hundred twenty-three children who underwent anti-reflux surgery were evaluated. Pyelonephritic scarring was more apt to occur with greater degrees of reflux. Postoperative infection was principally confined to the bladder and occurred primarily in females. The beneficial effect of anti-reflux surgery is discussed.", "contents": "Infection of the urinary tract after anti-reflux surgery. Two hundred twenty-three children who underwent anti-reflux surgery were evaluated. Pyelonephritic scarring was more apt to occur with greater degrees of reflux. Postoperative infection was principally confined to the bladder and occurred primarily in females. The beneficial effect of anti-reflux surgery is discussed.", "PMID": 978321}
{"id": "pubmed23n0032_11648", "title": "Defective phagocyte adherence in acute poststreptococcal glomerulonephritis: clinical and laboratory observations.", "content": "The adherence of circulating phagocytes to glass was studied in 15 children with acute poststreptococcal glomerulonephritis and in 27 healthy adults, 21 healthy children, and 14 children with normocomplementemic renal disease. The phagocytic adherence to glass in the patients with hypocomplementemic PSGN differed significantly from that of the control groups (p=less than 0.001). There was a positive correlation of phagocyte adherence with plasma C3 but not with plasma C4, C3, properdin factor B, severity of illness, or drugs administered. In addition, the adherence defect was present in two normocomplementemic PSGN patients. The defect gradually resolved in all patients with clinical improvement: it was useful as an index of recovery. The in vitro addition of functional C3 to whole blood produced the adherence defect in normal subjects and failed to correct the defect in patients with PSGN. It was postulated that a fragment of activated complement may have blocked a membrane receptor on these phagocytes and interfered with their adherence to glass.", "contents": "Defective phagocyte adherence in acute poststreptococcal glomerulonephritis: clinical and laboratory observations. The adherence of circulating phagocytes to glass was studied in 15 children with acute poststreptococcal glomerulonephritis and in 27 healthy adults, 21 healthy children, and 14 children with normocomplementemic renal disease. The phagocytic adherence to glass in the patients with hypocomplementemic PSGN differed significantly from that of the control groups (p=less than 0.001). There was a positive correlation of phagocyte adherence with plasma C3 but not with plasma C4, C3, properdin factor B, severity of illness, or drugs administered. In addition, the adherence defect was present in two normocomplementemic PSGN patients. The defect gradually resolved in all patients with clinical improvement: it was useful as an index of recovery. The in vitro addition of functional C3 to whole blood produced the adherence defect in normal subjects and failed to correct the defect in patients with PSGN. It was postulated that a fragment of activated complement may have blocked a membrane receptor on these phagocytes and interfered with their adherence to glass.", "PMID": 978322}
{"id": "pubmed23n0032_11649", "title": "Hyperphenylalaninemia due to dihydropteridine reductase deficiency. Assay of the enzyme in fibroblasts from affected infants, heterozygotes, and in normal amniotic fluid cells.", "content": "Two infant siblings with modest elevations of serum phenylalanine concentrations had seizures and developmental regression: they died in their second year. Dihydropteridine reductase activity, which can be measured in normal cultured skin fibroblasts, was measured in the younger sibling and was absent. Parents of the two siblings and parents of a previously reported patient all showed 50% or less of the normal dihydropteridine reductase activity in their cultured fibroblasts. Dihydropteridine reductase activity is also present in normal cultured amniotic fluid cells, offering the possibility of prenatal diagnosis. Absence of dihydropteridine reductase results not only in a defect in the conversion of phenylalaning to tyrosine, but also in the biosynthesis of the neurotransmitters, dopamine, norephinephrine, and serotonin. Since deficiencies in these neurotransmitters would not be alleviated by a phenylalanine-restricted diet, it is important to establish the nature of the enzymatic defect in all suspected variants of phenylketonuria.", "contents": "Hyperphenylalaninemia due to dihydropteridine reductase deficiency. Assay of the enzyme in fibroblasts from affected infants, heterozygotes, and in normal amniotic fluid cells. Two infant siblings with modest elevations of serum phenylalanine concentrations had seizures and developmental regression: they died in their second year. Dihydropteridine reductase activity, which can be measured in normal cultured skin fibroblasts, was measured in the younger sibling and was absent. Parents of the two siblings and parents of a previously reported patient all showed 50% or less of the normal dihydropteridine reductase activity in their cultured fibroblasts. Dihydropteridine reductase activity is also present in normal cultured amniotic fluid cells, offering the possibility of prenatal diagnosis. Absence of dihydropteridine reductase results not only in a defect in the conversion of phenylalaning to tyrosine, but also in the biosynthesis of the neurotransmitters, dopamine, norephinephrine, and serotonin. Since deficiencies in these neurotransmitters would not be alleviated by a phenylalanine-restricted diet, it is important to establish the nature of the enzymatic defect in all suspected variants of phenylketonuria.", "PMID": 978323}
{"id": "pubmed23n0032_11650", "title": "Enterovirus infections in neonates.", "content": "Twenty-seven ill neonates with enterovirus infections were studied to learn if enterovirus infection can be distinguished from neonatal sepsis. Enterovirus infection was associated with the summer-fall season (93%), recent maternal illness (59%), absence of other perinatal problems (81%), and findings of fever (93%), viral meningitis (62%), diarrhea (81%), and rash (41%). Four children developed thrombocytopenia and three necrotizing enterocolitis. Three children died, all with Coxsackie B virus infections that likely were acquired in utero. Although no single feature is pathognomonic, this constellation of epidemiologic and clinical findings, coupled with negative bacterial cultures, should suggest the possibility of neonatal enterovirus infection.", "contents": "Enterovirus infections in neonates. Twenty-seven ill neonates with enterovirus infections were studied to learn if enterovirus infection can be distinguished from neonatal sepsis. Enterovirus infection was associated with the summer-fall season (93%), recent maternal illness (59%), absence of other perinatal problems (81%), and findings of fever (93%), viral meningitis (62%), diarrhea (81%), and rash (41%). Four children developed thrombocytopenia and three necrotizing enterocolitis. Three children died, all with Coxsackie B virus infections that likely were acquired in utero. Although no single feature is pathognomonic, this constellation of epidemiologic and clinical findings, coupled with negative bacterial cultures, should suggest the possibility of neonatal enterovirus infection.", "PMID": 978329}
{"id": "pubmed23n0032_11651", "title": "Hypertension in infants--a complication of umbilical arterial catheterization.", "content": "Hypertension was observed in ten infants. Seven had thrombosis either of one renal artery or of both. The seven infants had previously had an indwelling catheter in the umbilical artery. One infant who had not had an indwelling umbilical arterial catheter had stenosis of a renal artery. No etiology for the hypertension was found in two infants, one of whom had had an umbilical arterial catheter. Response to antihypertensive medication was generally poor. Five of the infants died. We recommend prompt diagnostic evaluation in hypertensive infants. Nephrectomy should be considered, if the renal artery is occluded. Hypertension appears to be a life-threatening complication of umbilical arterial catheterization.", "contents": "Hypertension in infants--a complication of umbilical arterial catheterization. Hypertension was observed in ten infants. Seven had thrombosis either of one renal artery or of both. The seven infants had previously had an indwelling catheter in the umbilical artery. One infant who had not had an indwelling umbilical arterial catheter had stenosis of a renal artery. No etiology for the hypertension was found in two infants, one of whom had had an umbilical arterial catheter. Response to antihypertensive medication was generally poor. Five of the infants died. We recommend prompt diagnostic evaluation in hypertensive infants. Nephrectomy should be considered, if the renal artery is occluded. Hypertension appears to be a life-threatening complication of umbilical arterial catheterization.", "PMID": 978330}
{"id": "pubmed23n0032_11652", "title": "Relationships between maternal glucose intolerance and neonatal blood glucose.", "content": "Blood glucose changes in 63 infants during the first three hours of life were related to indices of glucose tolerance of their mothers. Of the mothers, 34 had insulin-dependent diabetes, 16 had gestational diabetes, and 11 had minor abnormalities of glucose tolerance. The fasting blood glucose level of the mother and the umbilical cord blood glucose level were both proportional to the rate of glucose decline in the infant after birth which, in turn, was inversely related to the lowest glucose level attained within three hours. Hypoglycemia occurred in 77% of the infants of diabetic mothers, 25% of the infants of mothers with gestational diabetes, and one of 12 (8%) of infants of mothers with minor degrees of glucose intolerance. The blood glucose level at two hours during an oral glucose tolerance test in the mother can be used to predict the probability of her infant having neonatal hypoglycemia.", "contents": "Relationships between maternal glucose intolerance and neonatal blood glucose. Blood glucose changes in 63 infants during the first three hours of life were related to indices of glucose tolerance of their mothers. Of the mothers, 34 had insulin-dependent diabetes, 16 had gestational diabetes, and 11 had minor abnormalities of glucose tolerance. The fasting blood glucose level of the mother and the umbilical cord blood glucose level were both proportional to the rate of glucose decline in the infant after birth which, in turn, was inversely related to the lowest glucose level attained within three hours. Hypoglycemia occurred in 77% of the infants of diabetic mothers, 25% of the infants of mothers with gestational diabetes, and one of 12 (8%) of infants of mothers with minor degrees of glucose intolerance. The blood glucose level at two hours during an oral glucose tolerance test in the mother can be used to predict the probability of her infant having neonatal hypoglycemia.", "PMID": 978332}
{"id": "pubmed23n0032_11653", "title": "Growth graphs for the clinical assessment of infants of varying gestational age.", "content": "Two graphs are presented showing means and 1 and 2 SD for growth in weight, length, and head circumference: (1) a fetal-infant graph with lines of growth from 26 weeks of gestational age until one year of age after \"\"term'' has been reached: and (2) a similarly constructed graph for children ages one through ten years. The standards of growth were obtained from published data in which the subjects received optimal health care. The graphs allow comparisons of infants of varying gestational age with standards for that age. Normal and abnormal deviations in growth are easily identified. Some examples of common variations in physical growth are described.", "contents": "Growth graphs for the clinical assessment of infants of varying gestational age. Two graphs are presented showing means and 1 and 2 SD for growth in weight, length, and head circumference: (1) a fetal-infant graph with lines of growth from 26 weeks of gestational age until one year of age after \"\"term'' has been reached: and (2) a similarly constructed graph for children ages one through ten years. The standards of growth were obtained from published data in which the subjects received optimal health care. The graphs allow comparisons of infants of varying gestational age with standards for that age. Normal and abnormal deviations in growth are easily identified. Some examples of common variations in physical growth are described.", "PMID": 978333}
{"id": "pubmed23n0032_11654", "title": "Reversible oxacillin hepatotoxicity.", "content": "Eight patients developed elevations of hepatic enzymes while receiving oxacillin intravenously. In all instances the patients were asymptomatic and anicteric. Peripheral eosinophilia was present in five of eight patients. In each patient change of therapy to a different pencillinase-resistant penicillin or to penicillin G was associated with a rapid decrease in liver function abnormalities and evenutal return of the enzymatic values to normal. Change of medication to an alternative penicillinase-resistant penicillin or to penicillin G is suggested as a safe procedure for completion of antistaphylococcal therapy in patient who develop oxacillin-related hepatotoxicity.", "contents": "Reversible oxacillin hepatotoxicity. Eight patients developed elevations of hepatic enzymes while receiving oxacillin intravenously. In all instances the patients were asymptomatic and anicteric. Peripheral eosinophilia was present in five of eight patients. In each patient change of therapy to a different pencillinase-resistant penicillin or to penicillin G was associated with a rapid decrease in liver function abnormalities and evenutal return of the enzymatic values to normal. Change of medication to an alternative penicillinase-resistant penicillin or to penicillin G is suggested as a safe procedure for completion of antistaphylococcal therapy in patient who develop oxacillin-related hepatotoxicity.", "PMID": 978335}
{"id": "pubmed23n0032_11655", "title": "Behavioural concomitants of prenatal addiction to narcotics.", "content": "Behavioral characteristics of infants of methadone-treated and nonaddicted women were studied during the neonatal period and at three, six, and 12 months of age. Several Brazelton Neonatal Behavioral Assment Scale measures differentiated between groups. Major differences occurred in irritability of the central nervous system, and these measures appeared able to predict severity of withdrawal. The increased tremulousness of addicted infants remained through at least the first month of life. Mental and motor development was within normal limits in both groups throughout the year. However, the addicted infants showed a progressive decline in psychomotor performance whereas nonaddicted infants' scores remained stable.", "contents": "Behavioural concomitants of prenatal addiction to narcotics. Behavioral characteristics of infants of methadone-treated and nonaddicted women were studied during the neonatal period and at three, six, and 12 months of age. Several Brazelton Neonatal Behavioral Assment Scale measures differentiated between groups. Major differences occurred in irritability of the central nervous system, and these measures appeared able to predict severity of withdrawal. The increased tremulousness of addicted infants remained through at least the first month of life. Mental and motor development was within normal limits in both groups throughout the year. However, the addicted infants showed a progressive decline in psychomotor performance whereas nonaddicted infants' scores remained stable.", "PMID": 978336}
{"id": "pubmed23n0032_11656", "title": "Mothers as volunteers in an oncology clinic.", "content": "Mothers of children who had died of cancer were successfully utilized as volunteers in a pediatric oncology clinic to provide emotional support and practical assistance to other children afflicted with the same disease and to their families. At the start of the program these volunteers were carefully interviewed to determine their willingness and their emotional ability to participate on a consistent basis. Following an eight-week training program with a psychiatrist, a social worker, and a nurse, they began working in a large pediatric oncology clinic one day a week. Over an 18-month period they have become valuable members of the oncology team by helping to improve communications, to alleviate frustrations, and in giving additional emotional support to the children and their families.", "contents": "Mothers as volunteers in an oncology clinic. Mothers of children who had died of cancer were successfully utilized as volunteers in a pediatric oncology clinic to provide emotional support and practical assistance to other children afflicted with the same disease and to their families. At the start of the program these volunteers were carefully interviewed to determine their willingness and their emotional ability to participate on a consistent basis. Following an eight-week training program with a psychiatrist, a social worker, and a nurse, they began working in a large pediatric oncology clinic one day a week. Over an 18-month period they have become valuable members of the oncology team by helping to improve communications, to alleviate frustrations, and in giving additional emotional support to the children and their families.", "PMID": 978337}
{"id": "pubmed23n0032_11657", "title": "Partial purification of amastigotes from cutaneous lesions of American leishmaniasis.", "content": "Amastigotes of Leishmania mexicana, L. mexicana amazonensis, L. brasiliensis, and L. enriettii were isolated from lesions in infected animals. Numbers of amastigotes recovered ranged from 1 X 10(7) to 7 X 10(8), depending on the strain of leishmania. Trypsinization disassociated the lesions and released the parasites. After 18 to 24 hr incubation at 37 C in tissue culture media with antibiotics, many of the intact host cells attached to the flask. Amastigotes were collected from the media in relatively pure preparations. Electron microscopy revealed no morphological alterations of the amastigotes and minimal contamination by membranes and cell fragments.", "contents": "Partial purification of amastigotes from cutaneous lesions of American leishmaniasis. Amastigotes of Leishmania mexicana, L. mexicana amazonensis, L. brasiliensis, and L. enriettii were isolated from lesions in infected animals. Numbers of amastigotes recovered ranged from 1 X 10(7) to 7 X 10(8), depending on the strain of leishmania. Trypsinization disassociated the lesions and released the parasites. After 18 to 24 hr incubation at 37 C in tissue culture media with antibiotics, many of the intact host cells attached to the flask. Amastigotes were collected from the media in relatively pure preparations. Electron microscopy revealed no morphological alterations of the amastigotes and minimal contamination by membranes and cell fragments.", "PMID": 978350}
{"id": "pubmed23n0032_11658", "title": "The genus Myxidium (Protozoa: Myxosporida) in macrourid fishes.", "content": "Five species, 3 new, of myoxosporida in the genus Myxidium were recovered from 7 genera and 23 species of deepsea macrourids. Although some Myxidium species were geographically dispersed they showed remarkable spore size stability. Two forms, \"long\" and \"stubby\" are described for M. coryphaenoidium. Abnormal M. coryphaenoidium spores are shown to resemble superficially other species and genera.", "contents": "The genus Myxidium (Protozoa: Myxosporida) in macrourid fishes. Five species, 3 new, of myoxosporida in the genus Myxidium were recovered from 7 genera and 23 species of deepsea macrourids. Although some Myxidium species were geographically dispersed they showed remarkable spore size stability. Two forms, \"long\" and \"stubby\" are described for M. coryphaenoidium. Abnormal M. coryphaenoidium spores are shown to resemble superficially other species and genera.", "PMID": 978351}
{"id": "pubmed23n0032_11659", "title": "Myxosporida (Protozoa) in California rockfish, Sebastes spp.", "content": "Six genera and 15 species, 2 new, of myxosporida were recovered from 14 species of California marine rockfish, Sebastes. The spores of Leptotheca sebasta sp. n. are arched with thick, equal shell valves and large round polar capsules. They are greater in width and sutural diameter and more crescentic than L. latesi. Ceratomyxa sebasta sp. n. is crescentic in shape with equal shell valves. The spores of C. sebasta are shorter in sutural diameter, larger in width, and less crescentic than C. hokarari.", "contents": "Myxosporida (Protozoa) in California rockfish, Sebastes spp. Six genera and 15 species, 2 new, of myxosporida were recovered from 14 species of California marine rockfish, Sebastes. The spores of Leptotheca sebasta sp. n. are arched with thick, equal shell valves and large round polar capsules. They are greater in width and sutural diameter and more crescentic than L. latesi. Ceratomyxa sebasta sp. n. is crescentic in shape with equal shell valves. The spores of C. sebasta are shorter in sutural diameter, larger in width, and less crescentic than C. hokarari.", "PMID": 978353}
{"id": "pubmed23n0032_11660", "title": "Responses of Megalodiscus temperatus miracidia to amino and sialic acids found in snail-conditioned water.", "content": "Analysis of snail-conditioned  water (SCW) from Helisoma trivolvis revealed 17 free amino acids. Those in great concentration were glycine, serine, and alanine. The concentration of sialic acid was found to be twice that of the most abundant amino acid. The behavior of miracidia of Megalodiscus temperatus, measured by the contact with return method, to agar cylinders containing single amino acids and sialic acid indicated greater responses to polar molecules charged either positively or negatively at neutral pH. The molecules elicting the greatest response were aspartic, glutamic, and sialic acid. No correlation was found between concentration of amino acids in H. trivolvis SCW and response of M. temperatus miracidia.", "contents": "Responses of Megalodiscus temperatus miracidia to amino and sialic acids found in snail-conditioned water. Analysis of snail-conditioned  water (SCW) from Helisoma trivolvis revealed 17 free amino acids. Those in great concentration were glycine, serine, and alanine. The concentration of sialic acid was found to be twice that of the most abundant amino acid. The behavior of miracidia of Megalodiscus temperatus, measured by the contact with return method, to agar cylinders containing single amino acids and sialic acid indicated greater responses to polar molecules charged either positively or negatively at neutral pH. The molecules elicting the greatest response were aspartic, glutamic, and sialic acid. No correlation was found between concentration of amino acids in H. trivolvis SCW and response of M. temperatus miracidia.", "PMID": 978354}
{"id": "pubmed23n0032_11661", "title": "The influence of temperature on the infectivity of Fasciola hepatica miracidia to Lymnaea truncatula.", "content": "Experiments were performed to study the effect of water temperature on the host-finding capacity (snail localization, attachment, and penetration) of Fasciola hepatica miracidia. Specimens of Lymnaea truncatula were exposed to miracidia labeled in vivo with radioselenium, and the radioactivity which subsequently was confined to the snails was taken as a measure of the host-finding capacity of the parasite. The minimum temperature required for host-finding was 5 to 6 C and the optimum temperature was in the range between 15 and 26 C. The lack of host-finding capacity below 5 to 6 C could be reversed experimentally by raising the temperature. A clear inverse relationship was demonstrated between the environnd 24 C the host-finding capacity ceased after 24 to 30, 20  to 24, and 13 to 20 hr, respectively.", "contents": "The influence of temperature on the infectivity of Fasciola hepatica miracidia to Lymnaea truncatula. Experiments were performed to study the effect of water temperature on the host-finding capacity (snail localization, attachment, and penetration) of Fasciola hepatica miracidia. Specimens of Lymnaea truncatula were exposed to miracidia labeled in vivo with radioselenium, and the radioactivity which subsequently was confined to the snails was taken as a measure of the host-finding capacity of the parasite. The minimum temperature required for host-finding was 5 to 6 C and the optimum temperature was in the range between 15 and 26 C. The lack of host-finding capacity below 5 to 6 C could be reversed experimentally by raising the temperature. A clear inverse relationship was demonstrated between the environnd 24 C the host-finding capacity ceased after 24 to 30, 20  to 24, and 13 to 20 hr, respectively.", "PMID": 978356}
{"id": "pubmed23n0032_11662", "title": "A redescription of Crassicutis archosargi, a digenean exhibiting an unusual tegumental attachment.", "content": "The homalometrine Crassicutis archosargi from the northern Gulf or Mexico is redescribed, and some related digeneans are discussed. Numerous unidentified refractile bodies occurred in the tegument and other tissues of a few specimens of C. archosargi, and an intimate relationship between the parasite and its piscine host resulting from a modified tegument is reported for others. Binding by the described adhesive tegument increases the number of known ways digeneans attach to their hosts. Several specimens also harbored a histozoic myxosporidan in several tissues and a flagellate, Hexamita sp., in their ceca.", "contents": "A redescription of Crassicutis archosargi, a digenean exhibiting an unusual tegumental attachment. The homalometrine Crassicutis archosargi from the northern Gulf or Mexico is redescribed, and some related digeneans are discussed. Numerous unidentified refractile bodies occurred in the tegument and other tissues of a few specimens of C. archosargi, and an intimate relationship between the parasite and its piscine host resulting from a modified tegument is reported for others. Binding by the described adhesive tegument increases the number of known ways digeneans attach to their hosts. Several specimens also harbored a histozoic myxosporidan in several tissues and a flagellate, Hexamita sp., in their ceca.", "PMID": 978357}
{"id": "pubmed23n0032_11663", "title": "Immunocytochemical studies on Schistosoma mansoni. II. Soluble cercarial antigens in cercarial and schistosomules.", "content": "The localization of soluble, cercarial antigen preparation (CAP) in cercariae and schistosomules of Schistosoma mansoni was performed at the light and electron microscope levels using the unlabeled antibody method. Reaction produce was observed associated with the contents of the pre- and postacetabular glands and with the filamentous coat of mature cercariae. No reaction product was observed associated with the glycoacalyx of schistosomules. However, several schistosomules did retain remnants of their filamentous coats and reaction product was observed associated with those remains. CAP components were also observed in the area surrounding the intrasporocyst cercariae.", "contents": "Immunocytochemical studies on Schistosoma mansoni. II. Soluble cercarial antigens in cercarial and schistosomules. The localization of soluble, cercarial antigen preparation (CAP) in cercariae and schistosomules of Schistosoma mansoni was performed at the light and electron microscope levels using the unlabeled antibody method. Reaction produce was observed associated with the contents of the pre- and postacetabular glands and with the filamentous coat of mature cercariae. No reaction product was observed associated with the glycoacalyx of schistosomules. However, several schistosomules did retain remnants of their filamentous coats and reaction product was observed associated with those remains. CAP components were also observed in the area surrounding the intrasporocyst cercariae.", "PMID": 978358}
{"id": "pubmed23n0032_11664", "title": "The eosinophilic response of the rat to infection with Taenia taeniaeformis.", "content": "Rats were dosed with eggs of Taenia taeniaeformis and hematologic parameters were measured throughout the course of primary infection. There was no evidence of anemia but differential leukocyte counts revealed distinct and reproducible patterns of white blood cell changes. A lymphocytosis developed at the end of the 1st and 5th weeks postinfection (p.i.). Neutrophil counts peaked 8 days p.i., although at that time there was no marked neutrophilic infiltration of the tissues. Eosinophil counts began to rise during the 2nd week p.i., and reached a peak during the 3rd week, followed by a decline and then another peak during the 5th week p.i. Eosinophilic infiltration of the tissues was remarkable during the period of peripheral eosinophilia. A wide zone of eosinophils surrounded the developing larvae at 22 days p.i. and persisted in some cases for a further 2 weeks. Eosinophils remained in lesser numbers in the connective tissue capsule throughout the infection, often in association with plasma cells. After oral challenge with 1,000 eggs infected rats showed brisk secondary eosinophilic responses 3 to 7 days later but other hematologic parameters were unaffected. Average peripheral eosinophil counts at 3 and 4 days post-challenge were significantly higher than those in unchallenged controls (P less than 0.05 and P less than 0.01, respectively). There was no detectable increase in eosinophilic infiltration of small intestinal tissues in challenged rats. These results are discussed in relation to current understanding of the mechanisms of eosinophil chemotaxis in vitro and the possible causes of local eosinophil accumulation in parasitic infections in vivo.", "contents": "The eosinophilic response of the rat to infection with Taenia taeniaeformis. Rats were dosed with eggs of Taenia taeniaeformis and hematologic parameters were measured throughout the course of primary infection. There was no evidence of anemia but differential leukocyte counts revealed distinct and reproducible patterns of white blood cell changes. A lymphocytosis developed at the end of the 1st and 5th weeks postinfection (p.i.). Neutrophil counts peaked 8 days p.i., although at that time there was no marked neutrophilic infiltration of the tissues. Eosinophil counts began to rise during the 2nd week p.i., and reached a peak during the 3rd week, followed by a decline and then another peak during the 5th week p.i. Eosinophilic infiltration of the tissues was remarkable during the period of peripheral eosinophilia. A wide zone of eosinophils surrounded the developing larvae at 22 days p.i. and persisted in some cases for a further 2 weeks. Eosinophils remained in lesser numbers in the connective tissue capsule throughout the infection, often in association with plasma cells. After oral challenge with 1,000 eggs infected rats showed brisk secondary eosinophilic responses 3 to 7 days later but other hematologic parameters were unaffected. Average peripheral eosinophil counts at 3 and 4 days post-challenge were significantly higher than those in unchallenged controls (P less than 0.05 and P less than 0.01, respectively). There was no detectable increase in eosinophilic infiltration of small intestinal tissues in challenged rats. These results are discussed in relation to current understanding of the mechanisms of eosinophil chemotaxis in vitro and the possible causes of local eosinophil accumulation in parasitic infections in vivo.", "PMID": 978361}
{"id": "pubmed23n0032_11665", "title": "Antibody-mediated secondary eosinophilic response to Taenia taeniaeformis in the rat.", "content": "Normal rats were given intravenous doses of either immune serum or immunoglobulin fractions 24 hr before oral challenge with 1,000 eggs of Taenia taeniaeformis. Total eosinophil counts per mm3 were performed for 3 days prior to and 6 days after challenge. Sensitized rats usually showed sharp peaks of eosinophilia 2 to 6 days after this dose. The pattern of the eosinophilic response was similar to that which occurs after challenge of immune infected rats. The differences in peripheral eosinophil levels in passively immunized and normal rats were statistically significant. Immunoglobulin fractions containing protective IgG2alpha were most effective, but a fraction containing reaginic antibody activity also sensitized rats to give secondary eosinophilic responses. The findings are discussed in relation to the probable contribution of antigen-antibody reactions to the production of secondary eosinophilic responses in experimental cysticercosis.", "contents": "Antibody-mediated secondary eosinophilic response to Taenia taeniaeformis in the rat. Normal rats were given intravenous doses of either immune serum or immunoglobulin fractions 24 hr before oral challenge with 1,000 eggs of Taenia taeniaeformis. Total eosinophil counts per mm3 were performed for 3 days prior to and 6 days after challenge. Sensitized rats usually showed sharp peaks of eosinophilia 2 to 6 days after this dose. The pattern of the eosinophilic response was similar to that which occurs after challenge of immune infected rats. The differences in peripheral eosinophil levels in passively immunized and normal rats were statistically significant. Immunoglobulin fractions containing protective IgG2alpha were most effective, but a fraction containing reaginic antibody activity also sensitized rats to give secondary eosinophilic responses. The findings are discussed in relation to the probable contribution of antigen-antibody reactions to the production of secondary eosinophilic responses in experimental cysticercosis.", "PMID": 978362}
{"id": "pubmed23n0032_11666", "title": "Metabolic indices for evaluating the in vitro maintenance of Hymenolepis diminuta in the presence and absence of various additives.", "content": "An in vitro maintenance system for H. diminuta was devised by modifying the cultivation procedure of Schiller (1965). In this diphasic maintenance system, tissue culture medium (Triple Eagle's or NCTC-135) was used, in lieu of whole blood, as the 30% supplement to the agar phase. This provides a more defined system suitable for studying the effects of various additives. Morphological criteria were established which aided in assessing the efficacy of media used for the maintenance of 6- and 8-day-old H. diminuta. When successfully maintained, worms exhibited an intact scolex and neck region, undulatory movements along the strobila as well as integumentary and strobilar integrity. A more sensitive method for evaluating the maintenance of 8-day-old worms employed metabolic indices. Wet weight, protein and glycogen levels for 8-day-old H. diminuta served as base-line data allowing estimation of protein and glycogen contents of each worm prior to maintenance. Following maintenance, ratio of final to initial protein and final to initial glycogen levels (metabolic indices). A metabolic index approaching or exceeding unity suggested a reasonably intact metabolism. The addition of sodium taurocholate to the maintenance media appeared beneficial to the worms by prolonging the retention of normal signs. A combination of additives, taurocholate-nucleosides-lipids, improved the maintenance of H. diminuta for periods exceeding 24 hr as determined by observational criteria and metabolic indices. However, addition of a lipid mixture, or a lipid mixture prepared with a low concentration of taurocholate was not beneficial over a 24 hr period. The maintenance system, observational criteria, base-line data and metabolic indices should be useful for future in vitro studies requiring long-term incubation.", "contents": "Metabolic indices for evaluating the in vitro maintenance of Hymenolepis diminuta in the presence and absence of various additives. An in vitro maintenance system for H. diminuta was devised by modifying the cultivation procedure of Schiller (1965). In this diphasic maintenance system, tissue culture medium (Triple Eagle's or NCTC-135) was used, in lieu of whole blood, as the 30% supplement to the agar phase. This provides a more defined system suitable for studying the effects of various additives. Morphological criteria were established which aided in assessing the efficacy of media used for the maintenance of 6- and 8-day-old H. diminuta. When successfully maintained, worms exhibited an intact scolex and neck region, undulatory movements along the strobila as well as integumentary and strobilar integrity. A more sensitive method for evaluating the maintenance of 8-day-old worms employed metabolic indices. Wet weight, protein and glycogen levels for 8-day-old H. diminuta served as base-line data allowing estimation of protein and glycogen contents of each worm prior to maintenance. Following maintenance, ratio of final to initial protein and final to initial glycogen levels (metabolic indices). A metabolic index approaching or exceeding unity suggested a reasonably intact metabolism. The addition of sodium taurocholate to the maintenance media appeared beneficial to the worms by prolonging the retention of normal signs. A combination of additives, taurocholate-nucleosides-lipids, improved the maintenance of H. diminuta for periods exceeding 24 hr as determined by observational criteria and metabolic indices. However, addition of a lipid mixture, or a lipid mixture prepared with a low concentration of taurocholate was not beneficial over a 24 hr period. The maintenance system, observational criteria, base-line data and metabolic indices should be useful for future in vitro studies requiring long-term incubation.", "PMID": 978363}
{"id": "pubmed23n0032_11667", "title": "The in vitro effects of farnesol and derivatives on Hymenolepis diminuta.", "content": "Employing an in vitro maintenance system, in which 8-day-old Hymenolepis diminuta survives for 24 hr (Fioravanti and MacInnis, 1976), it was found that farnesol or farnesal supplementation of the medium had no beneficial effects on maintenance and these substances induced necrosis at higher concentrations. Similar experiments utilizing Schiller's (1965) culture system demonstrated that neither farnesol, farnesal, nor farnesyl methyl ether exhibited growth promoting effects and were toxic to the worms at higher concentrations. In addition, neither the 2-cis, 6-trans nor the 2-trans, 6-trans-isomers of farnesol promoted growth in the Schiller system and at higher concentrations resulted in severe necrosis within 24 hr.", "contents": "The in vitro effects of farnesol and derivatives on Hymenolepis diminuta. Employing an in vitro maintenance system, in which 8-day-old Hymenolepis diminuta survives for 24 hr (Fioravanti and MacInnis, 1976), it was found that farnesol or farnesal supplementation of the medium had no beneficial effects on maintenance and these substances induced necrosis at higher concentrations. Similar experiments utilizing Schiller's (1965) culture system demonstrated that neither farnesol, farnesal, nor farnesyl methyl ether exhibited growth promoting effects and were toxic to the worms at higher concentrations. In addition, neither the 2-cis, 6-trans nor the 2-trans, 6-trans-isomers of farnesol promoted growth in the Schiller system and at higher concentrations resulted in severe necrosis within 24 hr.", "PMID": 978365}
{"id": "pubmed23n0032_11668", "title": "Putative neurosecretory cells of the cestode Hymenolepis microstoma.", "content": "Putative neurosecretory cells were observed with the electron microscope in the nerve cords of the neck region of Hymenolepis microstoma. The cells show evidence of glandular activity by the large numbers of dense-core vesicles produced by the Golgi apparatus. The axons contain synaptoidlike structures with surrounding clouds of vesicles; features analogous to known neurosecretory release sites.", "contents": "Putative neurosecretory cells of the cestode Hymenolepis microstoma. Putative neurosecretory cells were observed with the electron microscope in the nerve cords of the neck region of Hymenolepis microstoma. The cells show evidence of glandular activity by the large numbers of dense-core vesicles produced by the Golgi apparatus. The axons contain synaptoidlike structures with surrounding clouds of vesicles; features analogous to known neurosecretory release sites.", "PMID": 978366}
{"id": "pubmed23n0032_11669", "title": "The stichosome and its secretion granules in the mature muscle larva of Trichinella spiralis.", "content": "The stichosome of the mature muscle larva of Trichinella spiralis consists of a single row of 45 to 55 stichocytes. Each stichocyte is about 25 mum in diameter and possesses a single nucleus. A duct leads from each stichocyte to the lumen of the esophagus. The stichocyte cytoplasm contains mitochondria, structures resembling Golgi-complexes, rough endoplasmic reticulum, and usually 1 of 2 types of secretory granules. The alpha-granule measures about 800 mn in diameter, contains a prominent inclusion, and has a granular matrix. The beta-granule is about 600 mn in diameter and is homogeneous in appearance. Both granule types are surrounded by a single membrane. Ten to thirteen stichocytes containing alpha-granules are confined to the posterior portion of the stichosome. After isopycnic centrifugation in sucrose gradient of large granule fractions obtained from cell-free homogenates, the alpha- and beta-granules show characteristic distribution patterns as revealed by the morphology of the fractions. The median equilibrium density of the alpha-granules is 1.245, while that of the beta-granules is 1.230. There is a correlation between the distribution of the granules and of antigens reacting with hyperimmune antitrichinella seruma. At least 4 unique antigens can be attributed to each of the granule types. Fractions enriched in mitochondria do not contain specific antigens. Antigens from both types of secretory granules cross react totally with those present in the excretion-secretion products of living muscle larvae. Cytoimmunochemical data show that antigens are distributed in a patchy fashion throughout the stichocyte cytoplasm. This finding is consistent with the distribution of the secretory granules in the intact stichocyte.", "contents": "The stichosome and its secretion granules in the mature muscle larva of Trichinella spiralis. The stichosome of the mature muscle larva of Trichinella spiralis consists of a single row of 45 to 55 stichocytes. Each stichocyte is about 25 mum in diameter and possesses a single nucleus. A duct leads from each stichocyte to the lumen of the esophagus. The stichocyte cytoplasm contains mitochondria, structures resembling Golgi-complexes, rough endoplasmic reticulum, and usually 1 of 2 types of secretory granules. The alpha-granule measures about 800 mn in diameter, contains a prominent inclusion, and has a granular matrix. The beta-granule is about 600 mn in diameter and is homogeneous in appearance. Both granule types are surrounded by a single membrane. Ten to thirteen stichocytes containing alpha-granules are confined to the posterior portion of the stichosome. After isopycnic centrifugation in sucrose gradient of large granule fractions obtained from cell-free homogenates, the alpha- and beta-granules show characteristic distribution patterns as revealed by the morphology of the fractions. The median equilibrium density of the alpha-granules is 1.245, while that of the beta-granules is 1.230. There is a correlation between the distribution of the granules and of antigens reacting with hyperimmune antitrichinella seruma. At least 4 unique antigens can be attributed to each of the granule types. Fractions enriched in mitochondria do not contain specific antigens. Antigens from both types of secretory granules cross react totally with those present in the excretion-secretion products of living muscle larvae. Cytoimmunochemical data show that antigens are distributed in a patchy fashion throughout the stichocyte cytoplasm. This finding is consistent with the distribution of the secretory granules in the intact stichocyte.", "PMID": 978367}
{"id": "pubmed23n0032_11670", "title": "The Ornithodoros (Alectorobius) capensis group (Acarina: Ixodoidea: Argasidae) of the palearctic and oriental regions. O. (A.) maritimus: identity, marine bird hosts, virus infections, and distribution in western Europe and northwestern Africa.", "content": "In 1967, Vermeil and Marguet described Ornithodoros coniceps maritimus from larvae reared from larvae taken from marine birds on Dumet Island (Atlantic Ocean), Basse Bretagne, France. We collected O. (A.) coniceps Canestrini, 1890, from the type locality (Venice, Italy) and determined that the taxons coniceps and maritimus each require full species status. We selected a lectotype and paralectotypes for the taxon maritimus from the original Dumet Island material. The larva of maritimus is redescribed and the nymph, male, and female are described for the first time. Collection data are recorded from Dumet and other islands off France, Aegimures Islands off Tunisia, Puffin Island off northern Wales, and Great Saltee Island off Ireland. This tick infests nesting colonies of the common tern, roseate tern, sandwich tern, herring gull (northern and Mediterranean races), common cormorant, shag, razorbill, common murre, black-legged kittiwake, and probably other marine birds nesting nearby. Adults and nymphs (tentatively identified as maritimus but lacking associated larvae for full confirmation) were taken near nests of the little egret in Lake Tunis, Tunisia. Soldado virus was isolated from Puffin Island tick samples and a Soldado-like virus from Great Saltee Island tick samples. An experimental study of West Nile virus in the Tunisian tick population is reviewed. The birds species associated with maritimus in each collecting locality, and their nesting and resting habits and migration patterns in relation to tick and arbovirus survival and distribution, will be reported in the following paper in this series.", "contents": "The Ornithodoros (Alectorobius) capensis group (Acarina: Ixodoidea: Argasidae) of the palearctic and oriental regions. O. (A.) maritimus: identity, marine bird hosts, virus infections, and distribution in western Europe and northwestern Africa. In 1967, Vermeil and Marguet described Ornithodoros coniceps maritimus from larvae reared from larvae taken from marine birds on Dumet Island (Atlantic Ocean), Basse Bretagne, France. We collected O. (A.) coniceps Canestrini, 1890, from the type locality (Venice, Italy) and determined that the taxons coniceps and maritimus each require full species status. We selected a lectotype and paralectotypes for the taxon maritimus from the original Dumet Island material. The larva of maritimus is redescribed and the nymph, male, and female are described for the first time. Collection data are recorded from Dumet and other islands off France, Aegimures Islands off Tunisia, Puffin Island off northern Wales, and Great Saltee Island off Ireland. This tick infests nesting colonies of the common tern, roseate tern, sandwich tern, herring gull (northern and Mediterranean races), common cormorant, shag, razorbill, common murre, black-legged kittiwake, and probably other marine birds nesting nearby. Adults and nymphs (tentatively identified as maritimus but lacking associated larvae for full confirmation) were taken near nests of the little egret in Lake Tunis, Tunisia. Soldado virus was isolated from Puffin Island tick samples and a Soldado-like virus from Great Saltee Island tick samples. An experimental study of West Nile virus in the Tunisian tick population is reviewed. The birds species associated with maritimus in each collecting locality, and their nesting and resting habits and migration patterns in relation to tick and arbovirus survival and distribution, will be reported in the following paper in this series.", "PMID": 978368}
{"id": "pubmed23n0032_11671", "title": "Peripheral nervous system of the ticks, Amblyomma tuberculatum Marx and Argas radiatus Railliet (Acari: Ixodoidea).", "content": "The peripheral nervous system of an ixodid and an argasid tick include the following components: (1) paired optic, cheliceral and pedipalpal nerves and unpaired stomodeal nerve arising from the pre-esophageal portion of the central nervous system (synganglion); (2) 4 pairs of pedal nerve trunks (each with appendicular and hemal branches) and 4 major pairs of opisthosomal nerves (including the paraspiracular, genital, postlateral-myosomal, and ano-myosomal nerves); and (3) the lateral \"sympathetic\" plexuses formed by contributions of hemal nerves arising from pedal ganglia and from the paraspiracular nerves. The salivary glands are multiply innervated by 4 pairs of nerves. The 1st pair arise from the pedipalpal nerves, the 2nd and 3rd from the lateral sympathetic plexuses, and the 4th from the paraspiracular nerves. Portions of the integument and the majority of non-appendicular muscles are innervated by various fine nerves arising from the lateral sympathetic plexuses and from the 4 pairs of opisthosomal nerves.", "contents": "Peripheral nervous system of the ticks, Amblyomma tuberculatum Marx and Argas radiatus Railliet (Acari: Ixodoidea). The peripheral nervous system of an ixodid and an argasid tick include the following components: (1) paired optic, cheliceral and pedipalpal nerves and unpaired stomodeal nerve arising from the pre-esophageal portion of the central nervous system (synganglion); (2) 4 pairs of pedal nerve trunks (each with appendicular and hemal branches) and 4 major pairs of opisthosomal nerves (including the paraspiracular, genital, postlateral-myosomal, and ano-myosomal nerves); and (3) the lateral \"sympathetic\" plexuses formed by contributions of hemal nerves arising from pedal ganglia and from the paraspiracular nerves. The salivary glands are multiply innervated by 4 pairs of nerves. The 1st pair arise from the pedipalpal nerves, the 2nd and 3rd from the lateral sympathetic plexuses, and the 4th from the paraspiracular nerves. Portions of the integument and the majority of non-appendicular muscles are innervated by various fine nerves arising from the lateral sympathetic plexuses and from the 4 pairs of opisthosomal nerves.", "PMID": 978369}
{"id": "pubmed23n0032_11672", "title": "Study of neonatal gastric aspirate related to amniotic fluid and to the occurrence of the idiopathic respiratory distress syndrome.", "content": "The lecithin/sphingomyelin ratio (L/S) has been shown to be of use in the prediction of the idiopathic respiratory distress syndrome (I.R.D.S) when performed on amniotic fluid. Gastric contents at birth consist mainly of amniotic fluid. Our study was designed to determine what the relationship was between the L/S ratios of amniotic fluid and gastric aspirate obtained at birth and to examine whether the L/S ratios of gastric aspirate was of any value in the prediction of I.R.D.S.", "contents": "Study of neonatal gastric aspirate related to amniotic fluid and to the occurrence of the idiopathic respiratory distress syndrome. The lecithin/sphingomyelin ratio (L/S) has been shown to be of use in the prediction of the idiopathic respiratory distress syndrome (I.R.D.S) when performed on amniotic fluid. Gastric contents at birth consist mainly of amniotic fluid. Our study was designed to determine what the relationship was between the L/S ratios of amniotic fluid and gastric aspirate obtained at birth and to examine whether the L/S ratios of gastric aspirate was of any value in the prediction of I.R.D.S.", "PMID": 978381}
{"id": "pubmed23n0032_11673", "title": "Theoretical isotropic dissolution of nonspherical particles.", "content": "Equations are derived for the isotropic dissolution of single particles, considering simple forms of the six crystal systems. These can be summarized by three basic equations which are approximated well, and in some cases exactly, by the dissolution equation for a hypothetical spherical particle of specified diameter. Formulas are given to enable calculation of this diameter and to minimize the weighted errors in the approximations. Spherical approximations provide a simple basis for calculating the dissolution profile of real multiparticulate systems which are difficult to describe otherwise. Spherical approximations based on equal surface area or volume result in large errors.", "contents": "Theoretical isotropic dissolution of nonspherical particles. Equations are derived for the isotropic dissolution of single particles, considering simple forms of the six crystal systems. These can be summarized by three basic equations which are approximated well, and in some cases exactly, by the dissolution equation for a hypothetical spherical particle of specified diameter. Formulas are given to enable calculation of this diameter and to minimize the weighted errors in the approximations. Spherical approximations provide a simple basis for calculating the dissolution profile of real multiparticulate systems which are difficult to describe otherwise. Spherical approximations based on equal surface area or volume result in large errors.", "PMID": 978399}
{"id": "pubmed23n0032_11674", "title": "Experimental evaluation of three single-particle dissolution models.", "content": "The dissolution of the 60-85-mesh fraction os recording, flow-through dissolution apparatus equipped with a dissolution cell; it was particularly suitable for kinetic analysis of multiparticulate systems. By using a time-scaling approach, experimental data are compared with theoretical calculations to evaluate, quantitatively, which of three single-particle dissolution models best describes the data and how well the multiparticulate kinetics can be explained mathematically. The nonspherical tolbutamide particles are replaced in the calculations by a hypothetical system of spherical particles that appears to be log-normally distributed. This procedure permits the calculation of the intrinsic dissolution profile, considering both size distribution and particle shape effects.", "contents": "Experimental evaluation of three single-particle dissolution models. The dissolution of the 60-85-mesh fraction os recording, flow-through dissolution apparatus equipped with a dissolution cell; it was particularly suitable for kinetic analysis of multiparticulate systems. By using a time-scaling approach, experimental data are compared with theoretical calculations to evaluate, quantitatively, which of three single-particle dissolution models best describes the data and how well the multiparticulate kinetics can be explained mathematically. The nonspherical tolbutamide particles are replaced in the calculations by a hypothetical system of spherical particles that appears to be log-normally distributed. This procedure permits the calculation of the intrinsic dissolution profile, considering both size distribution and particle shape effects.", "PMID": 978400}
{"id": "pubmed23n0032_11675", "title": "Antioxidant efficiency of polyhydric phenols in photooxidation of benzaldehyde.", "content": "An experimental system is described for the observation  of the kinetics of photochemically initiated oxidation reactions; this system is based on the measurement of oxygen consumption with a polarographic oxygen electrode. The photooxidation of benzaldehyde in dilute aqueous solution was examined and appears to conform to a free radical chain mechanism. The antioxidant efficiency of some polyhydric phenols was determined kinetically and found to be catechol greather than pyrogallol greater than hydroquinone greather than resorcinol greater than n-propyl gallate for the benzaldehyde photooxidation.", "contents": "Antioxidant efficiency of polyhydric phenols in photooxidation of benzaldehyde. An experimental system is described for the observation  of the kinetics of photochemically initiated oxidation reactions; this system is based on the measurement of oxygen consumption with a polarographic oxygen electrode. The photooxidation of benzaldehyde in dilute aqueous solution was examined and appears to conform to a free radical chain mechanism. The antioxidant efficiency of some polyhydric phenols was determined kinetically and found to be catechol greather than pyrogallol greater than hydroquinone greather than resorcinol greater than n-propyl gallate for the benzaldehyde photooxidation.", "PMID": 978401}
{"id": "pubmed23n0032_11676", "title": "Photochromism of an anticonvulsant, 1-diphenylmethyl-4-(6-methyl-2-pyridylmethylene-amino) piperazine, in the solid state.", "content": "The light-induced color change of 1-diphenylmethyl-4-(6-methyl-2-pyridylmethyleneamino) piperazine in the solid state was investigated. Light in the 420-700-nm visible region had no effect. Elevated temperature, dissolution, or prolonged storage in the dark at room temperatures restored the intrinsic color of the compound. IR, UV (solution), NMR, differential scanning calorimetry, and GC methods showed no detectable difference between the long wave-length UV light-exposed (yellow) and unexposed (colorless) samples of the pure compound. Long wavelength UV light exposure studies with several substituted piperazine analogs revealed a structure-activity requirement for the color conversion process. The data indicated that the transformation process from colorless (or faint yellow) to bright yellow is photochromism (phototropy) and is dependent on the intensity of the \"action spectrum\" in the 300-400-nm region. Studies in the solid state showed that heat-induced fading of the color followed apparent zero-order kinetics. The energy of activation, Eb, for the photochromic conversion process from the metastable (yellow) to the stable (colorless) state was estimated to be about 19 kcal/mole.", "contents": "Photochromism of an anticonvulsant, 1-diphenylmethyl-4-(6-methyl-2-pyridylmethylene-amino) piperazine, in the solid state. The light-induced color change of 1-diphenylmethyl-4-(6-methyl-2-pyridylmethyleneamino) piperazine in the solid state was investigated. Light in the 420-700-nm visible region had no effect. Elevated temperature, dissolution, or prolonged storage in the dark at room temperatures restored the intrinsic color of the compound. IR, UV (solution), NMR, differential scanning calorimetry, and GC methods showed no detectable difference between the long wave-length UV light-exposed (yellow) and unexposed (colorless) samples of the pure compound. Long wavelength UV light exposure studies with several substituted piperazine analogs revealed a structure-activity requirement for the color conversion process. The data indicated that the transformation process from colorless (or faint yellow) to bright yellow is photochromism (phototropy) and is dependent on the intensity of the \"action spectrum\" in the 300-400-nm region. Studies in the solid state showed that heat-induced fading of the color followed apparent zero-order kinetics. The energy of activation, Eb, for the photochromic conversion process from the metastable (yellow) to the stable (colorless) state was estimated to be about 19 kcal/mole.", "PMID": 978402}
{"id": "pubmed23n0032_11677", "title": "Interaction of sodium alkyl sulfates with everted rat small intestinal membrane.", "content": "The effect of sodium alkyl sulfates (C6-C14) on the loss of protein from the everted rat small intestine was measured. At a surfactant concentration of 10 mM, a peak effect on protein release was noted with sodium decyl sulfate (C10). Measurement of proten release as a function of sidium lauryl sulfate (C12) concentration resulted in the observation that the effect appears to be due to the micellar phaseof the surfactant solution. At concentrations of C12 above the CMC, the loss of protein from the intestinal preparation increased as the concentration of surfactant was increased. There may be a maximum amount of protein that can be released from the everted rat small intestinal sacs by surface-active agents. At equivalent micellar concentrations of C8-C14 (12.5 times the CMC), there was no difference in the amount of protein released in the presence of the individual alkyl sulfate solutions. Other studies on salicylate transfer across the everted rat small intestine indicate that permeability changes occur with anionic and cationic surfactants but not with nonionics.", "contents": "Interaction of sodium alkyl sulfates with everted rat small intestinal membrane. The effect of sodium alkyl sulfates (C6-C14) on the loss of protein from the everted rat small intestine was measured. At a surfactant concentration of 10 mM, a peak effect on protein release was noted with sodium decyl sulfate (C10). Measurement of proten release as a function of sidium lauryl sulfate (C12) concentration resulted in the observation that the effect appears to be due to the micellar phaseof the surfactant solution. At concentrations of C12 above the CMC, the loss of protein from the intestinal preparation increased as the concentration of surfactant was increased. There may be a maximum amount of protein that can be released from the everted rat small intestinal sacs by surface-active agents. At equivalent micellar concentrations of C8-C14 (12.5 times the CMC), there was no difference in the amount of protein released in the presence of the individual alkyl sulfate solutions. Other studies on salicylate transfer across the everted rat small intestine indicate that permeability changes occur with anionic and cationic surfactants but not with nonionics.", "PMID": 978403}
{"id": "pubmed23n0032_11678", "title": "Automated fluorometric analysis of epinephrine in lidocaine hydrochloride injection.", "content": "A rapid automated method for determining epinephrine in lidocaine hydrochloride injection is presented. The method is an adaptation of the official USP XIX fluorometric procedure and yields equivalent results. The linearity, accuracy, precision, excipient/background effect, and carryover characteristics of the automated system are described. A recovery study indicated that intact epinephrine is measured accurately in the presence of its degradation products. Analysis of several commercial preparations is reported.", "contents": "Automated fluorometric analysis of epinephrine in lidocaine hydrochloride injection. A rapid automated method for determining epinephrine in lidocaine hydrochloride injection is presented. The method is an adaptation of the official USP XIX fluorometric procedure and yields equivalent results. The linearity, accuracy, precision, excipient/background effect, and carryover characteristics of the automated system are described. A recovery study indicated that intact epinephrine is measured accurately in the presence of its degradation products. Analysis of several commercial preparations is reported.", "PMID": 978404}
{"id": "pubmed23n0032_11679", "title": "Clofibrate microcapsules: preparation and release rat studies.", "content": "Microencapsulation of clofibrate and dissolution characteristics of clofibrate microcapsules were investigated. Spherical droplets of clofibrate, prepared by a capillary jet method, were encapsulated in gelatin by simpel coacervation, using sodium sulfate as the coacervating agent. The microcapsules, which were hardened up to 8 hr with formaldehyde, were recovered as discrete, free-flowing particles. Dissolution of clofibrate from the microcapsules was not adequately described by either square root of time or Langenbucher kinetics but followed predominantly zero-order release patterns at all hardening times. A linear correlation was found between the hardening times and the t50% release time.", "contents": "Clofibrate microcapsules: preparation and release rat studies. Microencapsulation of clofibrate and dissolution characteristics of clofibrate microcapsules were investigated. Spherical droplets of clofibrate, prepared by a capillary jet method, were encapsulated in gelatin by simpel coacervation, using sodium sulfate as the coacervating agent. The microcapsules, which were hardened up to 8 hr with formaldehyde, were recovered as discrete, free-flowing particles. Dissolution of clofibrate from the microcapsules was not adequately described by either square root of time or Langenbucher kinetics but followed predominantly zero-order release patterns at all hardening times. A linear correlation was found between the hardening times and the t50% release time.", "PMID": 978405}
{"id": "pubmed23n0032_11680", "title": "Synthesis of potential mescaline antagonists.", "content": "1-[2-(3,4,5-Trimethoxyphenyl)ethyl]-3-pyrroline, 2-(3,4,5-trimethoxybenzyl)-1,2,3,6-tetrahydropyridine, N-n-propylmescaline, N-cyclopropylmethylmescaline, and N-allylmescaline were synthesized as potential mescaline antagonists. The ability of these compounds to antagonize mescaline-induced disruption of swim behavior is also given.", "contents": "Synthesis of potential mescaline antagonists. 1-[2-(3,4,5-Trimethoxyphenyl)ethyl]-3-pyrroline, 2-(3,4,5-trimethoxybenzyl)-1,2,3,6-tetrahydropyridine, N-n-propylmescaline, N-cyclopropylmethylmescaline, and N-allylmescaline were synthesized as potential mescaline antagonists. The ability of these compounds to antagonize mescaline-induced disruption of swim behavior is also given.", "PMID": 978406}
{"id": "pubmed23n0032_11681", "title": "Dissolution behavior and bioavailability of phenytoin from a ground mixture with microcrystalline cellulose.", "content": "The ground mixture of phenytoin and microcrystalline cellulose was prepared by grinding in a vibrational ball mill. The X-ray diffraction patterns indicated the amorphous nature of the ground mixture. Comparative studies were made concerning the in vitro dissolution and in vivo absorption of fine phenytoin powder, phenytoin sodium powder, and the ground mixture. The ground mixture showed a greater dissolution rate than the fine powder and attained supersaturation in the pharmacopeial disintegration media at pH 1.2 and 7.4. In vivo absorption studies of each preparation were carried out in five subjects, using a crossover design, by measuring the urinary excretion rate of a main metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin. The blood levels of phenytoin and the corresponding urinary excretion patterns of the metabolite were determined in two subjects. The ground mixtures significantly improved the bioavailability of phenytoin. The drug was completely and rapidly absorbed after oral administration of the ground mixture. The vibrational ball milling technique for a poorly water-soluble drug with microcrystalline cellulose provides a promising way of improving the in vivo drug absorption.", "contents": "Dissolution behavior and bioavailability of phenytoin from a ground mixture with microcrystalline cellulose. The ground mixture of phenytoin and microcrystalline cellulose was prepared by grinding in a vibrational ball mill. The X-ray diffraction patterns indicated the amorphous nature of the ground mixture. Comparative studies were made concerning the in vitro dissolution and in vivo absorption of fine phenytoin powder, phenytoin sodium powder, and the ground mixture. The ground mixture showed a greater dissolution rate than the fine powder and attained supersaturation in the pharmacopeial disintegration media at pH 1.2 and 7.4. In vivo absorption studies of each preparation were carried out in five subjects, using a crossover design, by measuring the urinary excretion rate of a main metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin. The blood levels of phenytoin and the corresponding urinary excretion patterns of the metabolite were determined in two subjects. The ground mixtures significantly improved the bioavailability of phenytoin. The drug was completely and rapidly absorbed after oral administration of the ground mixture. The vibrational ball milling technique for a poorly water-soluble drug with microcrystalline cellulose provides a promising way of improving the in vivo drug absorption.", "PMID": 978407}
{"id": "pubmed23n0032_11682", "title": "Solubility of nonelectrolytes in polar solvents IV: nonpolar drugs in mixed solvents.", "content": "The molecular and group surface area approach to solubility is shown to be applicable to mixed aqueous solvent systems. An equation is derived which is consistent with the exponential increase in the aqueous solubility of nonpolar drugs that frequently accompanies the addition of a cosolvent. This equation predicts that: (a) the ability of a drug to be solubilized by a cosolvent is proportional to its hydrophobic surface area per molecule, and (b) the ability of a cosolvent to solubilize any drug is inversely proportional to its interfacial tension against a reference liquid hydrocarbon. These predictions are experimentally verified with solubility studies of several alkyl p-aminobenzoates in propylene glycol-water mixtures and of hexyl p-aminobenzoate in mixtures of water the ethanol, methanol, ethylene glycol, propylene glycol, glycerin, and formamide.", "contents": "Solubility of nonelectrolytes in polar solvents IV: nonpolar drugs in mixed solvents. The molecular and group surface area approach to solubility is shown to be applicable to mixed aqueous solvent systems. An equation is derived which is consistent with the exponential increase in the aqueous solubility of nonpolar drugs that frequently accompanies the addition of a cosolvent. This equation predicts that: (a) the ability of a drug to be solubilized by a cosolvent is proportional to its hydrophobic surface area per molecule, and (b) the ability of a cosolvent to solubilize any drug is inversely proportional to its interfacial tension against a reference liquid hydrocarbon. These predictions are experimentally verified with solubility studies of several alkyl p-aminobenzoates in propylene glycol-water mixtures and of hexyl p-aminobenzoate in mixtures of water the ethanol, methanol, ethylene glycol, propylene glycol, glycerin, and formamide.", "PMID": 978408}
{"id": "pubmed23n0032_11683", "title": "Bioavailability of 11 sulfisoxazole products in humans.", "content": "Single lots of 11 commercially available 500-mg sulfisoxazole tablets were evaluated in vitro and in vivo. All products tested met USP XVIII specifications for weight variation and product assay. However, three products failed to meet the USP XVIII dissolution requirement. The only statistically significant difference observed between the 11 products was a lower peak plasma level exhibited by one product. No useful correlation was observed between the in vivo and in vitro studies for the dosage forms tested.", "contents": "Bioavailability of 11 sulfisoxazole products in humans. Single lots of 11 commercially available 500-mg sulfisoxazole tablets were evaluated in vitro and in vivo. All products tested met USP XVIII specifications for weight variation and product assay. However, three products failed to meet the USP XVIII dissolution requirement. The only statistically significant difference observed between the 11 products was a lower peak plasma level exhibited by one product. No useful correlation was observed between the in vivo and in vitro studies for the dosage forms tested.", "PMID": 978409}
{"id": "pubmed23n0032_11684", "title": "Effect of certain drugs in perfused human placenta XII: autacoid antagonism by phenothiazines.", "content": "The effects of chlorpromazine, prochlorperazine, and trifluoperazine on the pressor actions of serotonin, angiotensin, and bradykinin in the perfused vessels of full-term human placentas were investigated. A significant inhibition of the effect of serotonin was observed with trifluoperazine and chlorpromazine but not with prochlorperazine. The inhibition is attributed to the ability of phenothiazines to cause adrenergic blockade. Because both bradykinin and angiotensin could not be consistently antagonized, it is concluded that they must act primarily via musculotropic mechanisms and only secondarily by stimulation of adrenergic receptors.", "contents": "Effect of certain drugs in perfused human placenta XII: autacoid antagonism by phenothiazines. The effects of chlorpromazine, prochlorperazine, and trifluoperazine on the pressor actions of serotonin, angiotensin, and bradykinin in the perfused vessels of full-term human placentas were investigated. A significant inhibition of the effect of serotonin was observed with trifluoperazine and chlorpromazine but not with prochlorperazine. The inhibition is attributed to the ability of phenothiazines to cause adrenergic blockade. Because both bradykinin and angiotensin could not be consistently antagonized, it is concluded that they must act primarily via musculotropic mechanisms and only secondarily by stimulation of adrenergic receptors.", "PMID": 978410}
{"id": "pubmed23n0032_11685", "title": "High-pressure liquid chromatographic analysis of tolbutamide in serum.", "content": "A high-pressure liquid chromatographic (HPLC) analysis of tolbutamide in serum is described. The assay requires only 1 ml of serum and is capable of measuring as little as 2 mug of tolbutamide. The metabolites of tolbutamide do not interfere in the assay. Human serum samples, taken after a 1-g oral dose of tolbutamide, were analyzed by the HPLC and an existing GLC procedure, and the results are compared.", "contents": "High-pressure liquid chromatographic analysis of tolbutamide in serum. A high-pressure liquid chromatographic (HPLC) analysis of tolbutamide in serum is described. The assay requires only 1 ml of serum and is capable of measuring as little as 2 mug of tolbutamide. The metabolites of tolbutamide do not interfere in the assay. Human serum samples, taken after a 1-g oral dose of tolbutamide, were analyzed by the HPLC and an existing GLC procedure, and the results are compared.", "PMID": 978411}
{"id": "pubmed23n0032_11686", "title": "Prodrug approaches to enhancement of physicochemical properties of drugs IV: novel epinephrine prodrug.", "content": "The synthesis and characterization of a prodrug that appears to overcome the problem of inefficient absorption of epinephrine through the lipoidal membranes of the eye are described. The enzymatic rate of regeneration of epinephrine from the prodrug was determined using a rabbit eye homogenate, rabbit plasma, and human plasma. The prodrug had no activity of its own when tested against a guinea pig smooth muscle preparation. Upon enzymatic regeneration of epinephrine from the prodrug, however, the reaction mixture exhibited alpha-adrenergic activity equivalent to that of epinephrine when tested in the same preparation.", "contents": "Prodrug approaches to enhancement of physicochemical properties of drugs IV: novel epinephrine prodrug. The synthesis and characterization of a prodrug that appears to overcome the problem of inefficient absorption of epinephrine through the lipoidal membranes of the eye are described. The enzymatic rate of regeneration of epinephrine from the prodrug was determined using a rabbit eye homogenate, rabbit plasma, and human plasma. The prodrug had no activity of its own when tested against a guinea pig smooth muscle preparation. Upon enzymatic regeneration of epinephrine from the prodrug, however, the reaction mixture exhibited alpha-adrenergic activity equivalent to that of epinephrine when tested in the same preparation.", "PMID": 978412}
{"id": "pubmed23n0032_11687", "title": "Mass spectral analysis of glucuronides from sympathomimetic hydroxyphenylalkylaminoethanols.", "content": "A mass spectral method is described for the structure determination of glucuronic acid conjugates of hydroxyphenylalkylaminoethanol-type drugs. Trimethylsilylation and application of the GLC-mass spectral technique yield mass spectra with sufficient information for the identification of all structural subunits.", "contents": "Mass spectral analysis of glucuronides from sympathomimetic hydroxyphenylalkylaminoethanols. A mass spectral method is described for the structure determination of glucuronic acid conjugates of hydroxyphenylalkylaminoethanol-type drugs. Trimethylsilylation and application of the GLC-mass spectral technique yield mass spectra with sufficient information for the identification of all structural subunits.", "PMID": 978413}
{"id": "pubmed23n0032_11688", "title": "In vitro release of therapeutically active ingredients from polymer matrixes.", "content": "Several therapeutically active ingredients including benzocaine, cyclomethycaine, and methapyrilene hydrochloride were incorporated into ethylcellulose and polyamide films. The effect of cetyl alcohol and tributyl citrate upon the release of these ingredients was studied. The films containing the active ingredient and plasticizer were cast upon a mercury substrate, and the in vitro release of these drugs from each film into a desorbing medium of distilled water was measured. The results indicated that the film-forming agent and plasticizer affected the drug release rate and that the release followed first-order kinetics. Benzocaine was slowly released from polyamide-cetyl alcohol films and polyamide-cetyl alcohol-tributyl citrate films. Polyamide-tributyl citrate films showed enhanced release of benzoacaine and cyclomethycaine. Ethylcellulose films plasticized with tributyl citrate produced a fast drug release. Based upon these results, a water-soluble, highly polar, noncomplexing additive would tend to increase the drug release from the film. When the amount of benzocaine released from ethylcellulose was plotted as a function of the square root of time, a linear plot was obtained. Since this linear plot passed through the origin, ethylcellulose should be an ideal matrix for benzocaine according to the Higuchi diffusion-controlled model. These studies demonstrated the in vitro release of thf the solubility of the active agent in both the polymer film as a function of the solubility of the active agent in both the polymer matrix and the desorbing medium.", "contents": "In vitro release of therapeutically active ingredients from polymer matrixes. Several therapeutically active ingredients including benzocaine, cyclomethycaine, and methapyrilene hydrochloride were incorporated into ethylcellulose and polyamide films. The effect of cetyl alcohol and tributyl citrate upon the release of these ingredients was studied. The films containing the active ingredient and plasticizer were cast upon a mercury substrate, and the in vitro release of these drugs from each film into a desorbing medium of distilled water was measured. The results indicated that the film-forming agent and plasticizer affected the drug release rate and that the release followed first-order kinetics. Benzocaine was slowly released from polyamide-cetyl alcohol films and polyamide-cetyl alcohol-tributyl citrate films. Polyamide-tributyl citrate films showed enhanced release of benzoacaine and cyclomethycaine. Ethylcellulose films plasticized with tributyl citrate produced a fast drug release. Based upon these results, a water-soluble, highly polar, noncomplexing additive would tend to increase the drug release from the film. When the amount of benzocaine released from ethylcellulose was plotted as a function of the square root of time, a linear plot was obtained. Since this linear plot passed through the origin, ethylcellulose should be an ideal matrix for benzocaine according to the Higuchi diffusion-controlled model. These studies demonstrated the in vitro release of thf the solubility of the active agent in both the polymer film as a function of the solubility of the active agent in both the polymer matrix and the desorbing medium.", "PMID": 978414}
{"id": "pubmed23n0032_11689", "title": "Determination of salicylates by GLC.", "content": "Silylation of salicylic acid by hexamethyldisilazane, N,O-bis(trimethylsilyl)acetamide, and N,N-bis(trimethylsilyl)trifluoroacetamide was compared using GLC. The completeness of the reaction, the stability of products, and the reproducibility of results with time were investigated. Different reaction vessels were examined for their reliability and application for a routine assay procedure.", "contents": "Determination of salicylates by GLC. Silylation of salicylic acid by hexamethyldisilazane, N,O-bis(trimethylsilyl)acetamide, and N,N-bis(trimethylsilyl)trifluoroacetamide was compared using GLC. The completeness of the reaction, the stability of products, and the reproducibility of results with time were investigated. Different reaction vessels were examined for their reliability and application for a routine assay procedure.", "PMID": 978415}
{"id": "pubmed23n0032_11690", "title": "High-performance liquid chromatographic separation of C-15-epimers of 15-methylprostaglandin E2 methyl ester and 15-methylprostaglandin E2.", "content": "A high-performance liquid chromatographic system that permits quantitation of 15-methylprostaglandin E2 and its methyl ester is described. Separation from the corresponding C-15 epimers is effected by adsorption chromatography on microparticulate silica with refractive index detection.", "contents": "High-performance liquid chromatographic separation of C-15-epimers of 15-methylprostaglandin E2 methyl ester and 15-methylprostaglandin E2. A high-performance liquid chromatographic system that permits quantitation of 15-methylprostaglandin E2 and its methyl ester is described. Separation from the corresponding C-15 epimers is effected by adsorption chromatography on microparticulate silica with refractive index detection.", "PMID": 978416}
{"id": "pubmed23n0032_11691", "title": "Comparison of observed and predicted first-pass metabolism of nortriptyline in humans.", "content": "The extent of first-pass metabolism of nortriptyline, calculated by comparing the areas under the plasma concentration-time curves following intravenous and oral dosing in six individuals, varied from 41 to 54%. Theoretically predicted values ranged from 41 to 61% based on a plasma flow model, indicating that the clearance takes place mainly from the plasma, which does not represent the whole blood concentration.", "contents": "Comparison of observed and predicted first-pass metabolism of nortriptyline in humans. The extent of first-pass metabolism of nortriptyline, calculated by comparing the areas under the plasma concentration-time curves following intravenous and oral dosing in six individuals, varied from 41 to 54%. Theoretically predicted values ranged from 41 to 61% based on a plasma flow model, indicating that the clearance takes place mainly from the plasma, which does not represent the whole blood concentration.", "PMID": 978417}
{"id": "pubmed23n0032_11692", "title": "Selectivity of 4-methoxyphenethylamine derivatives as inhibitors of monoamine oxidase.", "content": "It has been established that the oxidative deamination of tyramine by monoamine xodase is inhibited by (+/-)-4-methoxy-beta-hydroxyphenethylamine and its N-methylated derivatives. This particular series of compounds does not inhibit the action of monoamine oxidase when tryptamine is used as the substrate. In contrast, 4-methoxyphenethylamine and its N-methylated homologs inhibit the monoamine oxidase-catalyzed deamination of both tyramine and tryptamine.", "contents": "Selectivity of 4-methoxyphenethylamine derivatives as inhibitors of monoamine oxidase. It has been established that the oxidative deamination of tyramine by monoamine xodase is inhibited by (+/-)-4-methoxy-beta-hydroxyphenethylamine and its N-methylated derivatives. This particular series of compounds does not inhibit the action of monoamine oxidase when tryptamine is used as the substrate. In contrast, 4-methoxyphenethylamine and its N-methylated homologs inhibit the monoamine oxidase-catalyzed deamination of both tyramine and tryptamine.", "PMID": 978418}
{"id": "pubmed23n0032_11693", "title": "Volume of distribution and tissue level errors in instantaneous intravenous input assumptions.", "content": "A comparison was made between instantaneous input assumption and short-term zero-order infusion for 1 and 2 min for several drugs showing fast distribution to the tissue compartments. The errors ranged from 9 to 40% for the volume of the central compartment, from 0.7 to 11% for the volume of distribution following pseudo-distribution equilibrium, and from 11 to 34% for the volume of distribution change at the end of the 2-min infusion period. Significant errors in the tissue levels also were observed. It was suggested that all intravenous administrations be considered as short-term zero-order inputs.", "contents": "Volume of distribution and tissue level errors in instantaneous intravenous input assumptions. A comparison was made between instantaneous input assumption and short-term zero-order infusion for 1 and 2 min for several drugs showing fast distribution to the tissue compartments. The errors ranged from 9 to 40% for the volume of the central compartment, from 0.7 to 11% for the volume of distribution following pseudo-distribution equilibrium, and from 11 to 34% for the volume of distribution change at the end of the 2-min infusion period. Significant errors in the tissue levels also were observed. It was suggested that all intravenous administrations be considered as short-term zero-order inputs.", "PMID": 978419}
{"id": "pubmed23n0032_11694", "title": "Stereochemistries of geometric isomers of 4-(2-bromo-1,2-diphenylvinyl)phenol, 4-(2-bromo-1,2-diphenyylamine: corrections of the literature.", "content": "The stereochemistries of geometric isomers of 4-(2-bromo-1,2-diphenylvinyl)phenol, 4-(2-bromo-1,2-diphenylvinyl)anisole, and 2-[p-(2-bromo-1,2-diphenylvinyl)phenoxy]triethylamine were determined by conversion of the phenolic analog to the ethers and subsequent comparison of physical properties with those of 2-[p-(2-chloro-1,2-diphenylvinyl)phenoxy]triethylamine of known stereochemistry.", "contents": "Stereochemistries of geometric isomers of 4-(2-bromo-1,2-diphenylvinyl)phenol, 4-(2-bromo-1,2-diphenyylamine: corrections of the literature. The stereochemistries of geometric isomers of 4-(2-bromo-1,2-diphenylvinyl)phenol, 4-(2-bromo-1,2-diphenylvinyl)anisole, and 2-[p-(2-bromo-1,2-diphenylvinyl)phenoxy]triethylamine were determined by conversion of the phenolic analog to the ethers and subsequent comparison of physical properties with those of 2-[p-(2-chloro-1,2-diphenylvinyl)phenoxy]triethylamine of known stereochemistry.", "PMID": 978420}
{"id": "pubmed23n0032_11695", "title": "Antimicrobial activity of newly synthesized isothiocyanate derivatives against pathogenic plant microorganisms.", "content": "Fifteen reaction products of isothiocyanates with cysteine, seven reaction products of isothiocyanates with 2,3-dimercapto-1-propanol, and four reaction products of isothiocyanates with sulfanilamide were synthesized. Their antimicrobial activity against pathogenic plant microorganisms was investigated.", "contents": "Antimicrobial activity of newly synthesized isothiocyanate derivatives against pathogenic plant microorganisms. Fifteen reaction products of isothiocyanates with cysteine, seven reaction products of isothiocyanates with 2,3-dimercapto-1-propanol, and four reaction products of isothiocyanates with sulfanilamide were synthesized. Their antimicrobial activity against pathogenic plant microorganisms was investigated.", "PMID": 978421}
{"id": "pubmed23n0032_11696", "title": "Automated analysis of warfarin sodium tablets.", "content": "An automated procedure was developed for the determination of warfarin sodium by following the steps of the manual USP procedure. The automated procedure is applicable to single tablets and composites of 20 tablets at different tablet concentrations. Sensitivity, precision, accuracy, and reproducibility are equivalent to the manual USP procedure. Sensitivity was approximately 15 mug/ml, with a coefficient of variation of 0.711%.", "contents": "Automated analysis of warfarin sodium tablets. An automated procedure was developed for the determination of warfarin sodium by following the steps of the manual USP procedure. The automated procedure is applicable to single tablets and composites of 20 tablets at different tablet concentrations. Sensitivity, precision, accuracy, and reproducibility are equivalent to the manual USP procedure. Sensitivity was approximately 15 mug/ml, with a coefficient of variation of 0.711%.", "PMID": 978422}
{"id": "pubmed23n0032_11697", "title": "Sulfur analogs of psychotomimetic amines.", "content": "The syntheses and physical properties are described for 2,5-dimethoxy-4-methylthiophenylethylamine and 2,5-dimethoxy-4-methylthiophenylisopropylamine. The latter compound is the sulfur analog of the psychotomimetic phenylisopropylamines 2,4,5-trimethoxyphenylisopropylamine and 2,5-dimethoxy-4-methylphenylisopropylamine wherein the methylthio group replaces a methoxy group or a methyl group, respectively. This compound is predicted to be about 30 times as active as mescaline.", "contents": "Sulfur analogs of psychotomimetic amines. The syntheses and physical properties are described for 2,5-dimethoxy-4-methylthiophenylethylamine and 2,5-dimethoxy-4-methylthiophenylisopropylamine. The latter compound is the sulfur analog of the psychotomimetic phenylisopropylamines 2,4,5-trimethoxyphenylisopropylamine and 2,5-dimethoxy-4-methylphenylisopropylamine wherein the methylthio group replaces a methoxy group or a methyl group, respectively. This compound is predicted to be about 30 times as active as mescaline.", "PMID": 978423}
{"id": "pubmed23n0032_11698", "title": "Rapid procedure for assessment of compounds that modify uptake and release of tritiated norepinephrine.", "content": "A rapid procedure to estimate tritiated norepinephrine (levarterenol) in a single mouse heart is described. The method is based upon oxidation of the tritium in the tissue to tritiated water, which is then determined by liquid scintillation spectroscopy. Large numbers of samples can be assayed with great facility. The effects of standard compounds that modify the uptake and release of 3H-norepinephrine in heart tissue were determined with this system, and a procedure for studying their interactions is described.", "contents": "Rapid procedure for assessment of compounds that modify uptake and release of tritiated norepinephrine. A rapid procedure to estimate tritiated norepinephrine (levarterenol) in a single mouse heart is described. The method is based upon oxidation of the tritium in the tissue to tritiated water, which is then determined by liquid scintillation spectroscopy. Large numbers of samples can be assayed with great facility. The effects of standard compounds that modify the uptake and release of 3H-norepinephrine in heart tissue were determined with this system, and a procedure for studying their interactions is described.", "PMID": 978424}
{"id": "pubmed23n0032_11699", "title": "Critical analysis of \"flip-flop\" phenomenon in two-compartment pharmacokinetic model.", "content": "Computer simulations were used to examine the effect of first-order absorption on the disposition of one- and two-compartment model drugs. Two-compartment systems that attain a clinically acceptable beta-phase after rapid intravenous injection were perturbed by introduction of drug via first-order absorption. The validity of perceiving such a system as a potential \"flip-flop\" model was tested by comparing the negative slopes of log-linear plasma-time profiles to known values for ka and beta for various values of ka, k12, k21, and k10. Although most log-linear plots showed excellent correlation coefficients (r2 greater than 0.996), their negative slopes (S) did not represent either ka or beta under various combinations. A similar consideration of the one-compartment model enabled a comparison to be made between the two systems. Maximum negative errors were observed for both one- and two-compartment drugs as ka leads to k2 or beta, respectively. The value for S provided a good estimate of the absorption rate constant, ka, when k2 greater than or equal 2ka (one compartment) or beta greater than or equal 2ka. The elimination rate constant (k2 or beta) could be obtained from S for all one-compartment and some two-compartment drugs when the value of ka was approximately twice that of k2 or beta. Large positive errors also were observed with certain two-compartment drugs where the ratio of the four rate constants apparently linearized a nonlinear plasma profile. Conditions wherein S may be expected to approach beta wherein S approaches ka are clearly defined.", "contents": "Critical analysis of \"flip-flop\" phenomenon in two-compartment pharmacokinetic model. Computer simulations were used to examine the effect of first-order absorption on the disposition of one- and two-compartment model drugs. Two-compartment systems that attain a clinically acceptable beta-phase after rapid intravenous injection were perturbed by introduction of drug via first-order absorption. The validity of perceiving such a system as a potential \"flip-flop\" model was tested by comparing the negative slopes of log-linear plasma-time profiles to known values for ka and beta for various values of ka, k12, k21, and k10. Although most log-linear plots showed excellent correlation coefficients (r2 greater than 0.996), their negative slopes (S) did not represent either ka or beta under various combinations. A similar consideration of the one-compartment model enabled a comparison to be made between the two systems. Maximum negative errors were observed for both one- and two-compartment drugs as ka leads to k2 or beta, respectively. The value for S provided a good estimate of the absorption rate constant, ka, when k2 greater than or equal 2ka (one compartment) or beta greater than or equal 2ka. The elimination rate constant (k2 or beta) could be obtained from S for all one-compartment and some two-compartment drugs when the value of ka was approximately twice that of k2 or beta. Large positive errors also were observed with certain two-compartment drugs where the ratio of the four rate constants apparently linearized a nonlinear plasma profile. Conditions wherein S may be expected to approach beta wherein S approaches ka are clearly defined.", "PMID": 978432}
{"id": "pubmed23n0032_11700", "title": "Wettability of pharmaceutical solids: estimates of solid surface polarity.", "content": "Contact angle measurements of water and methylene iodide with various organic solids were used to estimate the surface free energy per square centimeter, gammaS, and the contributions of nonpolar and polar forces, gammaSd and gammaSp, respectively. The ratio of gammaSp to gammaS was used as an estimate of solid surface polarity, and values ranging from 0 to 42% polarity were calculated for materials of pharmaceutical interest. Surface free energies per mole were calculated to compare the influence of different substituent groups on wettability. This approach offers a convenient means to quantitate the polarity of organic pharmaceutical solid surfaces.", "contents": "Wettability of pharmaceutical solids: estimates of solid surface polarity. Contact angle measurements of water and methylene iodide with various organic solids were used to estimate the surface free energy per square centimeter, gammaS, and the contributions of nonpolar and polar forces, gammaSd and gammaSp, respectively. The ratio of gammaSp to gammaS was used as an estimate of solid surface polarity, and values ranging from 0 to 42% polarity were calculated for materials of pharmaceutical interest. Surface free energies per mole were calculated to compare the influence of different substituent groups on wettability. This approach offers a convenient means to quantitate the polarity of organic pharmaceutical solid surfaces.", "PMID": 978433}
{"id": "pubmed23n0032_11701", "title": "Comparative evaluation of tablet lubricants: effect of application method on tablet hardness and ejectability after compression.", "content": "The effect of four lubricants (stearic acid, magnesium stearate, calcium stearate, and talc) on the ejectability and hardness of statically compressed tablets of a lactose granulation was examined. Two methods of application of these lubricants, incorporation into the granulation and mixing with the granulation, were compared. In both methods, the energy consumption curves during ejection and the hardness curves with lubricant concentration were similar and showed complicated behavior at a lower concentration. The mixing method gave better results for ejectability and hardness than the incorporation method.", "contents": "Comparative evaluation of tablet lubricants: effect of application method on tablet hardness and ejectability after compression. The effect of four lubricants (stearic acid, magnesium stearate, calcium stearate, and talc) on the ejectability and hardness of statically compressed tablets of a lactose granulation was examined. Two methods of application of these lubricants, incorporation into the granulation and mixing with the granulation, were compared. In both methods, the energy consumption curves during ejection and the hardness curves with lubricant concentration were similar and showed complicated behavior at a lower concentration. The mixing method gave better results for ejectability and hardness than the incorporation method.", "PMID": 978434}
{"id": "pubmed23n0032_11702", "title": "Kinetic analysis of penicillin degradation in acidic media.", "content": "Degradation of penicillin in acidic media (pH 2.7) was monitored by high-pressure liquid chromatography and UV spectroscopy. The effects of temperature, buffer concentration, and ionic strength were examined. A degradation pathway is proposed, and the apparent first-order rate constant and energy of activation were calculated for each reaction. One or more degradation products containing a sulfhydryl group, a functional group often suggested as having a major role in eliciting allergic responses to penicillin therapy, were present throughout the degradation scheme.", "contents": "Kinetic analysis of penicillin degradation in acidic media. Degradation of penicillin in acidic media (pH 2.7) was monitored by high-pressure liquid chromatography and UV spectroscopy. The effects of temperature, buffer concentration, and ionic strength were examined. A degradation pathway is proposed, and the apparent first-order rate constant and energy of activation were calculated for each reaction. One or more degradation products containing a sulfhydryl group, a functional group often suggested as having a major role in eliciting allergic responses to penicillin therapy, were present throughout the degradation scheme.", "PMID": 978435}
{"id": "pubmed23n0032_11703", "title": "Effect of intragranular and extragranular disintegrating agents on particle size of disintegrated tablets.", "content": "Five materials were compared for their effectiveness as disintegrating agents: maize starch, sodium calcium alginate, alginic acid, microcrystalline cellulose, and a colloidal aluminum silicate. The effect of the proportion of the agent present and the position with respect to the granule, intra- and extragranular, was examined. The extragranular formulations disintegrated much more rapidly than the intragranular ones, but the latter gave a much finer dispersion of particles. A combination of intra- and extragranular disintegrating agents gave the best compromise; of those tested, the alginates appeared to effect the breakdown to the smallest particles when placed intragranularly. A method of assessing the effectiveness of disintegrating agents for uncoated tablets is suggested, but the resulting weight mean particle size is the more important criterion for tablets complying with a pharmacopoeial disintegration test. The porosity and crushing strength of tablets are useful as guides to disintegration only when a given formulation is used.", "contents": "Effect of intragranular and extragranular disintegrating agents on particle size of disintegrated tablets. Five materials were compared for their effectiveness as disintegrating agents: maize starch, sodium calcium alginate, alginic acid, microcrystalline cellulose, and a colloidal aluminum silicate. The effect of the proportion of the agent present and the position with respect to the granule, intra- and extragranular, was examined. The extragranular formulations disintegrated much more rapidly than the intragranular ones, but the latter gave a much finer dispersion of particles. A combination of intra- and extragranular disintegrating agents gave the best compromise; of those tested, the alginates appeared to effect the breakdown to the smallest particles when placed intragranularly. A method of assessing the effectiveness of disintegrating agents for uncoated tablets is suggested, but the resulting weight mean particle size is the more important criterion for tablets complying with a pharmacopoeial disintegration test. The porosity and crushing strength of tablets are useful as guides to disintegration only when a given formulation is used.", "PMID": 978436}
{"id": "pubmed23n0032_11704", "title": "Quantitative determination of theophylline in pharmaceutical dosage forms by differential spectrophotometry.", "content": "Theophylline was determined with good precision in tablets and capsules by differential spectrophotometry. Xanthines such as caffeine and theobromine did not interfere providing the total xanthine concentration was kept below 100 mug/ml. At a higher total xanthine concentration, nonlinearity occurred, presumably due to complex formation. This interference could be minimized by proper selection of the analytical wavelength.", "contents": "Quantitative determination of theophylline in pharmaceutical dosage forms by differential spectrophotometry. Theophylline was determined with good precision in tablets and capsules by differential spectrophotometry. Xanthines such as caffeine and theobromine did not interfere providing the total xanthine concentration was kept below 100 mug/ml. At a higher total xanthine concentration, nonlinearity occurred, presumably due to complex formation. This interference could be minimized by proper selection of the analytical wavelength.", "PMID": 978437}
{"id": "pubmed23n0032_11705", "title": "Physical aspects of wet granulations II: factors involved in prolonged and excessive mixing.", "content": "Evidence is presented that excessive blending in a wet granulation process shifts the packing arrangement of the wet granule, causing it to become dense and nonporous. With prolonged kneading, a large amount of the water-soluble excipients dissolves in the granulating fluid, and these two factors make the drying slower. This result, coupled with the previous finding that a certain time is required to attain an equilibrium size granule, explains why there exists an optimum kneading time for a wet granulation from a mechanical performance point of view.", "contents": "Physical aspects of wet granulations II: factors involved in prolonged and excessive mixing. Evidence is presented that excessive blending in a wet granulation process shifts the packing arrangement of the wet granule, causing it to become dense and nonporous. With prolonged kneading, a large amount of the water-soluble excipients dissolves in the granulating fluid, and these two factors make the drying slower. This result, coupled with the previous finding that a certain time is required to attain an equilibrium size granule, explains why there exists an optimum kneading time for a wet granulation from a mechanical performance point of view.", "PMID": 978438}
{"id": "pubmed23n0032_11706", "title": "Pharmaceutical applications of solid dispersion systems: dissolution of griseofulvin-succinic acid eutectic mixture.", "content": "The dissolution profile of the griseofulvin-succinic acid eutectic mixture system was evaluated using the powder and constant-surface are tablet methods. Factors contributing to the enhancement of griseofulvin dissolution from the dispersion in succinic acid are discussed. Contrary to the original proposal of Sekiguchi and coworkers, dissolution rates of driseofulvin from solid dispersions were found to be markedly affected by the particle size of solid dispersions.", "contents": "Pharmaceutical applications of solid dispersion systems: dissolution of griseofulvin-succinic acid eutectic mixture. The dissolution profile of the griseofulvin-succinic acid eutectic mixture system was evaluated using the powder and constant-surface are tablet methods. Factors contributing to the enhancement of griseofulvin dissolution from the dispersion in succinic acid are discussed. Contrary to the original proposal of Sekiguchi and coworkers, dissolution rates of driseofulvin from solid dispersions were found to be markedly affected by the particle size of solid dispersions.", "PMID": 978439}
{"id": "pubmed23n0032_11707", "title": "Application of trichloroacetyl isocyanate to NMR analysis of steroids of pharmaceutical interest I: corticosteroids and chemically related compounds.", "content": "The trichloroacetyl carbamates of 38 corticosteroids and chemically related compounds were prepared, and their NMR spectra in deuterochloroform were obtained. The effects of the introduction of a number of functional groups on the chemical shift of the carbamate proton signals were determined.", "contents": "Application of trichloroacetyl isocyanate to NMR analysis of steroids of pharmaceutical interest I: corticosteroids and chemically related compounds. The trichloroacetyl carbamates of 38 corticosteroids and chemically related compounds were prepared, and their NMR spectra in deuterochloroform were obtained. The effects of the introduction of a number of functional groups on the chemical shift of the carbamate proton signals were determined.", "PMID": 978440}
{"id": "pubmed23n0032_11708", "title": "Drug biotransformation interactions in man VI: acetaminophen and ascorbic acid.", "content": "Oral administration of 3 g of ascorbic acid 1.5 hr after an oral dose of 1 g of acetaminophen caused a rapid and pronounced decrease in the excretion rate of acetaminophen sulfate in five healthy adult volunteers. There was a statistically significant increase in the fractions of the dose of acetaminophen excreted as such as as acetaminophen glucuronide but a decrease in the fraction excreted as acetaminophen sulfate. The apparent biological half-life of acetaminophen increased from 2.3 +/- 0.2 (mean +/- SD) to 3.1 +/- 0.5 hr. Concomitant administration of sodium sulfate prevented these effects. Ascorbic acid, which itself is metabolized in part to the sulfate, inhibits the conjugation of acetaminophen with sulfate by competing for available sulfate in the body.", "contents": "Drug biotransformation interactions in man VI: acetaminophen and ascorbic acid. Oral administration of 3 g of ascorbic acid 1.5 hr after an oral dose of 1 g of acetaminophen caused a rapid and pronounced decrease in the excretion rate of acetaminophen sulfate in five healthy adult volunteers. There was a statistically significant increase in the fractions of the dose of acetaminophen excreted as such as as acetaminophen glucuronide but a decrease in the fraction excreted as acetaminophen sulfate. The apparent biological half-life of acetaminophen increased from 2.3 +/- 0.2 (mean +/- SD) to 3.1 +/- 0.5 hr. Concomitant administration of sodium sulfate prevented these effects. Ascorbic acid, which itself is metabolized in part to the sulfate, inhibits the conjugation of acetaminophen with sulfate by competing for available sulfate in the body.", "PMID": 978441}
{"id": "pubmed23n0032_11709", "title": "Structural functions of taste in the sugar series VII: taste properties of ketoses.", "content": "The preferable structural feature for sweetness is a lipophilic moiety, e.g., a five- or six-membered ring with a polar substituent containing an A-H/B system outside the ring. In ketoses, this unit is probably the 1,2-glycol. If a third feature (the lipophilic, gamma, site) is required for the attainment of optimum sweetness and if it is C-6 in ketoses, then this site can accommodate quite a large constituent, both above and below the plane of the ring. The removal of the hydroxyl group from the C-6 hydroxymethyl substituent to yield the 7-deoxy derivatives causes bitterness, thus implicating the primary hydroxymethyl group with bitterness.Therefore, the creation of lipophilic site(s) in the sugar ring causes the realignment of the sugar molecule on the taste receptor surface. The distrubance of the proposed A-H/B system, e.g., the removal of the C-2 hydroxyl group, causes the ring alpha-glycol unit (most likely the ring C-3 and C-4 hydroxyl groups) to function as the A-H/B system.", "contents": "Structural functions of taste in the sugar series VII: taste properties of ketoses. The preferable structural feature for sweetness is a lipophilic moiety, e.g., a five- or six-membered ring with a polar substituent containing an A-H/B system outside the ring. In ketoses, this unit is probably the 1,2-glycol. If a third feature (the lipophilic, gamma, site) is required for the attainment of optimum sweetness and if it is C-6 in ketoses, then this site can accommodate quite a large constituent, both above and below the plane of the ring. The removal of the hydroxyl group from the C-6 hydroxymethyl substituent to yield the 7-deoxy derivatives causes bitterness, thus implicating the primary hydroxymethyl group with bitterness.Therefore, the creation of lipophilic site(s) in the sugar ring causes the realignment of the sugar molecule on the taste receptor surface. The distrubance of the proposed A-H/B system, e.g., the removal of the C-2 hydroxyl group, causes the ring alpha-glycol unit (most likely the ring C-3 and C-4 hydroxyl groups) to function as the A-H/B system.", "PMID": 978442}
{"id": "pubmed23n0032_11710", "title": "Molecular connectivity V: connectivity series concept applied to density.", "content": "The concept of the expanded series of the connectivity index, chi, is introduced and applied to a consideration of the density of three classes of molecules. Correlations are found using two terms in the expanded series. A preliminary reflection on the extended series terms is made. It is noted that the regression equation constant in the three studies is close to the phase volume, 0.7402, and the possible significance of this fact is discussed.", "contents": "Molecular connectivity V: connectivity series concept applied to density. The concept of the expanded series of the connectivity index, chi, is introduced and applied to a consideration of the density of three classes of molecules. Correlations are found using two terms in the expanded series. A preliminary reflection on the extended series terms is made. It is noted that the regression equation constant in the three studies is close to the phase volume, 0.7402, and the possible significance of this fact is discussed.", "PMID": 978443}
{"id": "pubmed23n0032_11711", "title": "Computerized automated system for determining dissolution rate profiles for solid dosage forms.", "content": "A unique automated system was developed for the measurement of dissolution rates of tablets and capsules. This system, which adheres to principles set forth in the compendia, can test six dosage forms simultaneously. It is modular in form and capable of maintaining sink conditions, and it can handle any type of chemistry amenable to an automatic analyzer. In addition to the usual dissolution apparatus, the system includes a 12-channel combination sampling and solvent addition pump, a sequence control module, pertinent automated analyzer components, a spectrophotometer or fluorometer, an optional recorder, a specially designed digitizing system, and a teletype equipped with a paper tape punch reader and acoustic coupler. Each dissolution flask is automatically sampled every 6 min. Standards may be run either before or after the samples. Since dissolution, sampling, chemistry, and readout are all accomplished simultaneously, the complete test time is essentially reduced to the dissolution time itself. At the completion of sample dissolution, raw dissolution profile data are on the punched paper tape ready for computer processing via a time-sharing system. Developed software provides for the printout of a complete test report in less than 15 min.", "contents": "Computerized automated system for determining dissolution rate profiles for solid dosage forms. A unique automated system was developed for the measurement of dissolution rates of tablets and capsules. This system, which adheres to principles set forth in the compendia, can test six dosage forms simultaneously. It is modular in form and capable of maintaining sink conditions, and it can handle any type of chemistry amenable to an automatic analyzer. In addition to the usual dissolution apparatus, the system includes a 12-channel combination sampling and solvent addition pump, a sequence control module, pertinent automated analyzer components, a spectrophotometer or fluorometer, an optional recorder, a specially designed digitizing system, and a teletype equipped with a paper tape punch reader and acoustic coupler. Each dissolution flask is automatically sampled every 6 min. Standards may be run either before or after the samples. Since dissolution, sampling, chemistry, and readout are all accomplished simultaneously, the complete test time is essentially reduced to the dissolution time itself. At the completion of sample dissolution, raw dissolution profile data are on the punched paper tape ready for computer processing via a time-sharing system. Developed software provides for the printout of a complete test report in less than 15 min.", "PMID": 978444}
{"id": "pubmed23n0032_11712", "title": "Sensitive GLC procedure simultaneous determination of phenytoin and its major metabolite from plasma following single doses of phenytoin.", "content": "An improved GLC procedure was developed for the simultaneous determination of phenytoin and its metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin, in plasma and urine following enzyme hydrolysis. After extraction, the drug, the metabolite, and the internal standard, 5-(p-methylphenyl)-5-phenylhydantoin, are measured by GLC with flame-ionization detection as their respective methyl derivatives following flash-heater methylation with trimethylanilinium hydroxide. The drug and metabolite give well-resolved symmetrical peaks on a phenyl methyl silicone column, and the method has a sensitivity of 150 ng/ml of phenytoin and 125 ng/ml of the metabolite. GLC-mass spectral evidence is presented for the formation and intact determination of methyl derivatives of the drug, its metabolite, and the internal standard.", "contents": "Sensitive GLC procedure simultaneous determination of phenytoin and its major metabolite from plasma following single doses of phenytoin. An improved GLC procedure was developed for the simultaneous determination of phenytoin and its metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin, in plasma and urine following enzyme hydrolysis. After extraction, the drug, the metabolite, and the internal standard, 5-(p-methylphenyl)-5-phenylhydantoin, are measured by GLC with flame-ionization detection as their respective methyl derivatives following flash-heater methylation with trimethylanilinium hydroxide. The drug and metabolite give well-resolved symmetrical peaks on a phenyl methyl silicone column, and the method has a sensitivity of 150 ng/ml of phenytoin and 125 ng/ml of the metabolite. GLC-mass spectral evidence is presented for the formation and intact determination of methyl derivatives of the drug, its metabolite, and the internal standard.", "PMID": 978445}
{"id": "pubmed23n0032_11713", "title": "Synthesis of 1,2-dioleoyl-3-(alpha-14C-1-adamantoyl)-sn-glycerol and 1-14C-adamantanecarboxylic acid.", "content": "The pancreatic lipase inhibitor 1,2-dioleoyl-3-(alpha-14C-1-adamantoyl)-sn-glycerol, with a specific activity of 8 mCi/mmole, was prepared by consecutive acylation of 1,2-isopropylidene-sn-glycerol with 1-14C-adamantanecarboxylic acid chloride and oleoyl chloride. The 14C-labeled acid was conveniently prepared by carboxylation of 1-adamantanol using 14C-sodium formate in concentrated sulfuric acid.", "contents": "Synthesis of 1,2-dioleoyl-3-(alpha-14C-1-adamantoyl)-sn-glycerol and 1-14C-adamantanecarboxylic acid. The pancreatic lipase inhibitor 1,2-dioleoyl-3-(alpha-14C-1-adamantoyl)-sn-glycerol, with a specific activity of 8 mCi/mmole, was prepared by consecutive acylation of 1,2-isopropylidene-sn-glycerol with 1-14C-adamantanecarboxylic acid chloride and oleoyl chloride. The 14C-labeled acid was conveniently prepared by carboxylation of 1-adamantanol using 14C-sodium formate in concentrated sulfuric acid.", "PMID": 978446}
{"id": "pubmed23n0032_11714", "title": "Anticonvulsant activity of enzyme inhibitors in rats.", "content": "Three liver microsomal enzyme inhibitors, proadifen, 2,4-dichloro-6-phenylphenoxyethyldiethylamine, and 2,4-dichloro-6-phenylphenoxyethylamine, and a hepatotoxic agent, carbon tetrachloride, were tested for anticonvulsant activity in adult male albino rats using the maximal electroshock seizure technique. All four substances exhibited significant anticonvulsant activity 1 hr after intraperitoneal administration. This protection was absent when tested 24 hr later.", "contents": "Anticonvulsant activity of enzyme inhibitors in rats. Three liver microsomal enzyme inhibitors, proadifen, 2,4-dichloro-6-phenylphenoxyethyldiethylamine, and 2,4-dichloro-6-phenylphenoxyethylamine, and a hepatotoxic agent, carbon tetrachloride, were tested for anticonvulsant activity in adult male albino rats using the maximal electroshock seizure technique. All four substances exhibited significant anticonvulsant activity 1 hr after intraperitoneal administration. This protection was absent when tested 24 hr later.", "PMID": 978447}
{"id": "pubmed23n0032_11715", "title": "Antiradiation compounds XVI: N-heterocyclic aminoethyl disulfides and aminoethanethiosulfuric acids.", "content": "A series of N-heterocyclic and N-heterocyclic alkyl derivatives of bis(2-aminoethyl) disulfide and aminoethanethiosulfuric acid was synthesized as potential antiradiation and anticancer agents. The compounds were prepared by the reactions of the heterocyclic halides with bis(2-aminoethyl) disulfide and aminoethanethiosulfuric acid. A dithio acid derivative, 3,3-dimercapto-2-cyanoacryloylpyrrolidide, was also prepared. Several compounds, including the dithio acid derivative, provided good radiation protection to mice. None of the compounds screened showed appreciable anticancer activities in two leukemia systems.", "contents": "Antiradiation compounds XVI: N-heterocyclic aminoethyl disulfides and aminoethanethiosulfuric acids. A series of N-heterocyclic and N-heterocyclic alkyl derivatives of bis(2-aminoethyl) disulfide and aminoethanethiosulfuric acid was synthesized as potential antiradiation and anticancer agents. The compounds were prepared by the reactions of the heterocyclic halides with bis(2-aminoethyl) disulfide and aminoethanethiosulfuric acid. A dithio acid derivative, 3,3-dimercapto-2-cyanoacryloylpyrrolidide, was also prepared. Several compounds, including the dithio acid derivative, provided good radiation protection to mice. None of the compounds screened showed appreciable anticancer activities in two leukemia systems.", "PMID": 978448}
{"id": "pubmed23n0032_11716", "title": "Gravimetric determination of chlorhexidine using tetraphenylborate ion.", "content": "A precise and accurate gravimetric procedure was developed for the determination of chlorhexidine diacetate, digluconate, or dihydrochloride. Sodium tetraphenylborate solution was the precipitant in an acidic medium (pH 1). Tablets containing both chlorhexidine diacetate and benzocaine also were assayed.", "contents": "Gravimetric determination of chlorhexidine using tetraphenylborate ion. A precise and accurate gravimetric procedure was developed for the determination of chlorhexidine diacetate, digluconate, or dihydrochloride. Sodium tetraphenylborate solution was the precipitant in an acidic medium (pH 1). Tablets containing both chlorhexidine diacetate and benzocaine also were assayed.", "PMID": 978449}
{"id": "pubmed23n0032_11717", "title": "GLC determination of cyclobenzaprine in plasma and urine.", "content": "A GLC determination of cyclobenzaprine in human plasma and urine is described. After extraction from alkalinized samples with heptane-isopentyl alcohol (97:3), the drug and internal standard were back-extracted into 0.1 N HCl and then reextracted into ether. Use of a lower homolog of the drug as an internal standard was effective in reducing variability. Drug concentrations as low as 25 ng/ml could be assayed with high precision. Plasma levels in humans given 40 mg po or iv ranged from 5 to 51 ng/ml; little unchanged cyclobenzaprine was present in the urine. The N-desmethyl analog of the drug was detected as a metabolite in urine.", "contents": "GLC determination of cyclobenzaprine in plasma and urine. A GLC determination of cyclobenzaprine in human plasma and urine is described. After extraction from alkalinized samples with heptane-isopentyl alcohol (97:3), the drug and internal standard were back-extracted into 0.1 N HCl and then reextracted into ether. Use of a lower homolog of the drug as an internal standard was effective in reducing variability. Drug concentrations as low as 25 ng/ml could be assayed with high precision. Plasma levels in humans given 40 mg po or iv ranged from 5 to 51 ng/ml; little unchanged cyclobenzaprine was present in the urine. The N-desmethyl analog of the drug was detected as a metabolite in urine.", "PMID": 978450}
{"id": "pubmed23n0032_11718", "title": "IR spectroscopic characterization of 2-thiohydantoins and 2-thiobarbiturates.", "content": "A characterization of 2-thiohydantoins and 2-thiobarbiturates by IR spectra is proposed, using three characteristic group frequencies: the \"thioureide band\" around 1500 cm-1 and the antisymmetric-symmetric stretching modes of NCS bonds around 1400 and 1200 cm-1. The general characteristic absorption areas are found by comparison with N-phenylthioureas and thioanilides.", "contents": "IR spectroscopic characterization of 2-thiohydantoins and 2-thiobarbiturates. A characterization of 2-thiohydantoins and 2-thiobarbiturates by IR spectra is proposed, using three characteristic group frequencies: the \"thioureide band\" around 1500 cm-1 and the antisymmetric-symmetric stretching modes of NCS bonds around 1400 and 1200 cm-1. The general characteristic absorption areas are found by comparison with N-phenylthioureas and thioanilides.", "PMID": 978451}
{"id": "pubmed23n0032_11719", "title": "On the statistics of drug-receptor constants for partial agonists.", "content": "A linear relationship between equieffective concentrations of agonist in the presence and absence of partial agonist was examined statistically. These experimental concentrations permit the estimation of the apparent dissociation constant Kp with a weighted fit. The form of the weights was proposed theoretically and substantiated by simulated experiments. Constants Kp for (+/-)-p-chloroisoproterenol were estimated from the antagonism by three concentrations of (+/-)-p-chloroisoproterenol of the positive chronotropic effects of (-)-isoproterenol on kitten (Kp = 1.75 X 10(-7) M) and guinea-pig (Kp = 1.84 X 10(-7) M) right atria. The Kp values were similar regardless of the concentrations of (+/-)-p-chloroisoproterenol used; they were also similar to a Kp of 1.95 X 10(-7) M) estimated BY Kaumann and Birnbaumer (J. Biol. Chem. 249: 7874-7885, 1974) for the (+/-)-p-chloroisoproterenol-receptor complex coupled to the adenylyl cyclase in cell-free membrane particles of kitten heart. The proposed statistical procedure appears to be useful for systems with sigmoid log concentration-effect curves in which concentrations of the dependent variable are obtained from interpolation.", "contents": "On the statistics of drug-receptor constants for partial agonists. A linear relationship between equieffective concentrations of agonist in the presence and absence of partial agonist was examined statistically. These experimental concentrations permit the estimation of the apparent dissociation constant Kp with a weighted fit. The form of the weights was proposed theoretically and substantiated by simulated experiments. Constants Kp for (+/-)-p-chloroisoproterenol were estimated from the antagonism by three concentrations of (+/-)-p-chloroisoproterenol of the positive chronotropic effects of (-)-isoproterenol on kitten (Kp = 1.75 X 10(-7) M) and guinea-pig (Kp = 1.84 X 10(-7) M) right atria. The Kp values were similar regardless of the concentrations of (+/-)-p-chloroisoproterenol used; they were also similar to a Kp of 1.95 X 10(-7) M) estimated BY Kaumann and Birnbaumer (J. Biol. Chem. 249: 7874-7885, 1974) for the (+/-)-p-chloroisoproterenol-receptor complex coupled to the adenylyl cyclase in cell-free membrane particles of kitten heart. The proposed statistical procedure appears to be useful for systems with sigmoid log concentration-effect curves in which concentrations of the dependent variable are obtained from interpolation.", "PMID": 978455}
{"id": "pubmed23n0032_11720", "title": "The role of acetylcholine receptors and acetylcholinesterase activity in the development of denervation supersensitivity.", "content": "Strips of muscle from innervated and denervated rat hemidiaphragm were tested for sensitivity to acetylcholine and to carbachol. For both agonists, denervation (6-8 days) produced notable supersensitivity. However, the increase in sensitivity to acetylcholine (ca. 600-fold) was much greater than that to carbachol (ca. 51-fold). Denervation also produced an increase in [3H]alpha-bungarotoxin binding (ca. 20-fold), presumably indicative of an increase in the number of acetylcholine receptors. In addition to causing increases in tissue sensitivity and receptor number, denervation caused a marked loss of acetylcholinesterase activity (ca. 70%) and a modest loss of butyrylcholinesterase activity (ca. 20%). When innervated muscle was pretreated with eserine (5 X 10(-5) M), there was a loss of acetylcholinesterase activity (ca. 86%) and butyrylcholinesterase activity (ca. 36%). Simultaneously, there was an increase in tissue sensitivity to acetylcholine (ca. 26-fold). When denervated muscle was pretreated with eserine, there was no loss of enzyme activity beyond that caused by denervation. Furthermore, eserine pretreatment did not increase denervated muscle sensitivity to acetylcholine. The data suggest that both an increase in acetylcholine receptors and a decrease in acetylcholinesterase activity contribute to the phenomenon of denervation supersensitivity.", "contents": "The role of acetylcholine receptors and acetylcholinesterase activity in the development of denervation supersensitivity. Strips of muscle from innervated and denervated rat hemidiaphragm were tested for sensitivity to acetylcholine and to carbachol. For both agonists, denervation (6-8 days) produced notable supersensitivity. However, the increase in sensitivity to acetylcholine (ca. 600-fold) was much greater than that to carbachol (ca. 51-fold). Denervation also produced an increase in [3H]alpha-bungarotoxin binding (ca. 20-fold), presumably indicative of an increase in the number of acetylcholine receptors. In addition to causing increases in tissue sensitivity and receptor number, denervation caused a marked loss of acetylcholinesterase activity (ca. 70%) and a modest loss of butyrylcholinesterase activity (ca. 20%). When innervated muscle was pretreated with eserine (5 X 10(-5) M), there was a loss of acetylcholinesterase activity (ca. 86%) and butyrylcholinesterase activity (ca. 36%). Simultaneously, there was an increase in tissue sensitivity to acetylcholine (ca. 26-fold). When denervated muscle was pretreated with eserine, there was no loss of enzyme activity beyond that caused by denervation. Furthermore, eserine pretreatment did not increase denervated muscle sensitivity to acetylcholine. The data suggest that both an increase in acetylcholine receptors and a decrease in acetylcholinesterase activity contribute to the phenomenon of denervation supersensitivity.", "PMID": 978456}
{"id": "pubmed23n0032_11721", "title": "Stimulation of glycogenolysis by beta adrenergic agonists in skeletal muscle of mice with the phosphorylase kinase deficiency mutation (I strain).", "content": "The mechanism by which beta adrenergic agonist stimulate glycogenolysis in intact skeletal muscle was investigated in mice with the phosphorylase kinase deficiency mutation (I strain). Although extracts of I strain diaphragm muscle had only 3.7% of the phosphorylase kinase activity found in extracts of the control strain (C57BL), incubation of I strain hemidiaphragms in Krebs-Ringer bicarbonate buffer with either isoproterenol or epinephrine resulted in a stimulation of the rate of glycogenolysis. In C57BL diaphragms, the EC50 values for isoproterenol and epinephrine were 2 and 14 nM, respectively. With I strain diaphragms, dl-isoproterenol or l-epinephrine stimulated glycogenolysis as a linear function of the log of the drug concentration with no apparent plateau of response up to concentrations of 30 to 40 mugM. For each 10-fold increase in drug concentration, isoproterenol and epinephrine stimulated glycogenolysis in I strain muscles an additional 0.37 to 0.42 mg/g/hr, a slope in the concentration-response relationship of 0.17 and 0.37, respectively, of that measured in C57BL diaphragms at concentrations around the EC50. The highest glycogenolytic response measured in I strain hemidiaphragms (at 40 mugM isoproterenol) was 80% of the maximal catecholamine-stimulated glycogenolysis in C57BL diaphragms. Both 4 nM and 4 mugM isoproterenol, in a concentration-dependent manner, stimulated phosphorylase b to a conversion in I and C57BL diaphragms and increased cyclic adenosine 3':5'-monophosphate (cyclic AMP) concentrations. The glycogenolytic response to 10.1 nM dl-isoproterenol in both I and C57BL diaphragms was blocked by 34 nM l-propranolol but not by 34 nM d-propranolol. The response to 4 mugM isoproterenol was enhanced by the cyclic nucleotide phosphodiesterase inhibitors papaverine (27 mugM) or dl-4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone (Ro 20-1724, 3 mugM). From the results of these studies, we conclude: 1) Catecholamines stimulate glycogenolysis in skeletal muscle of I mice, as in C57BL mice, by interacting with the beta adrenergic receptor, thereby increasing tissue cyclic AMP concentrations and stimulating phosphorylase b to a conversion. 2) alternative hypotheses for the mechanism of the catecholamine-stimulated decrease in glycogen concentration in I skeletal muscle-inhibition of glycogen synthesis, hyposia and 5'-AMP stimulation of phosphorylase b activity-have been ruled out. 3) the activity of the mutant phosphorylase kinase, although it is only 3.7% of that in extracts of C57BL muscle, is sufficient to produce phosphorylase b to a conversion and thereby account for the glycogenolytic response of I strain muscle to catecholamines.", "contents": "Stimulation of glycogenolysis by beta adrenergic agonists in skeletal muscle of mice with the phosphorylase kinase deficiency mutation (I strain). The mechanism by which beta adrenergic agonist stimulate glycogenolysis in intact skeletal muscle was investigated in mice with the phosphorylase kinase deficiency mutation (I strain). Although extracts of I strain diaphragm muscle had only 3.7% of the phosphorylase kinase activity found in extracts of the control strain (C57BL), incubation of I strain hemidiaphragms in Krebs-Ringer bicarbonate buffer with either isoproterenol or epinephrine resulted in a stimulation of the rate of glycogenolysis. In C57BL diaphragms, the EC50 values for isoproterenol and epinephrine were 2 and 14 nM, respectively. With I strain diaphragms, dl-isoproterenol or l-epinephrine stimulated glycogenolysis as a linear function of the log of the drug concentration with no apparent plateau of response up to concentrations of 30 to 40 mugM. For each 10-fold increase in drug concentration, isoproterenol and epinephrine stimulated glycogenolysis in I strain muscles an additional 0.37 to 0.42 mg/g/hr, a slope in the concentration-response relationship of 0.17 and 0.37, respectively, of that measured in C57BL diaphragms at concentrations around the EC50. The highest glycogenolytic response measured in I strain hemidiaphragms (at 40 mugM isoproterenol) was 80% of the maximal catecholamine-stimulated glycogenolysis in C57BL diaphragms. Both 4 nM and 4 mugM isoproterenol, in a concentration-dependent manner, stimulated phosphorylase b to a conversion in I and C57BL diaphragms and increased cyclic adenosine 3':5'-monophosphate (cyclic AMP) concentrations. The glycogenolytic response to 10.1 nM dl-isoproterenol in both I and C57BL diaphragms was blocked by 34 nM l-propranolol but not by 34 nM d-propranolol. The response to 4 mugM isoproterenol was enhanced by the cyclic nucleotide phosphodiesterase inhibitors papaverine (27 mugM) or dl-4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone (Ro 20-1724, 3 mugM). From the results of these studies, we conclude: 1) Catecholamines stimulate glycogenolysis in skeletal muscle of I mice, as in C57BL mice, by interacting with the beta adrenergic receptor, thereby increasing tissue cyclic AMP concentrations and stimulating phosphorylase b to a conversion. 2) alternative hypotheses for the mechanism of the catecholamine-stimulated decrease in glycogen concentration in I skeletal muscle-inhibition of glycogen synthesis, hyposia and 5'-AMP stimulation of phosphorylase b activity-have been ruled out. 3) the activity of the mutant phosphorylase kinase, although it is only 3.7% of that in extracts of C57BL muscle, is sufficient to produce phosphorylase b to a conversion and thereby account for the glycogenolytic response of I strain muscle to catecholamines.", "PMID": 978457}
{"id": "pubmed23n0032_11722", "title": "Serotonin accumulation in the guinea-pig myenteric plexus: ion dependence, structure-activity relationship and the effect of drugs.", "content": "The specific accumulation of serotonin in neurons of the guinea-pig myenteric plexus was examined. Nonadrenergic neurons have been shown to be responsible for this accumulation. Serotonin accumulation, known to be sodium-dependent, was found to be inhibited by elevating the external potassium concentration. Accumulation was inversely related to the concentration of potassium and was also decreased in the presence of 0 mM Ca++ or 12 mM Ca++. The affinity of analogous molecules for the myenteric plexus was greatly reduced in compounds which had no alkyl amino side chain, in which the amino group was methylated or which had no 5-hydroxyl group. Most analogs competitively inhibited serotonin accumulation and 6-hydroxytryptamine was demonstrated by histofluorescence to be taken up into the myenteric plexus after chemical sympathectomy with 6-hydroxydopamine. Serotonin accumulation was also inhibited by tricyclic antidepressants and amphetamines. The inhibition of serotonin accumulation by these compounds differed from their inhibition of  accumulation of norepinephrine. As in the central nervous system, chlorimipramine was the most potent tricyclic antidepressant against accumulation of serotonin while desmethylimipramine was the most potent inhibitor of the accumulation of norepinephrine. Amphetamines were more effective inhibitors of serotonin accumulation than were tricyclic antidepressants, but all of these drugs were more effective against the accumulation of norepinephrine than serotonin. This study confirms the existence of a unique population of axons int he mammalian myenteric plexus which are distinguishable by their characteristic accumulation of serotonin and have not been found elsewhere in the peripheral nervous system.", "contents": "Serotonin accumulation in the guinea-pig myenteric plexus: ion dependence, structure-activity relationship and the effect of drugs. The specific accumulation of serotonin in neurons of the guinea-pig myenteric plexus was examined. Nonadrenergic neurons have been shown to be responsible for this accumulation. Serotonin accumulation, known to be sodium-dependent, was found to be inhibited by elevating the external potassium concentration. Accumulation was inversely related to the concentration of potassium and was also decreased in the presence of 0 mM Ca++ or 12 mM Ca++. The affinity of analogous molecules for the myenteric plexus was greatly reduced in compounds which had no alkyl amino side chain, in which the amino group was methylated or which had no 5-hydroxyl group. Most analogs competitively inhibited serotonin accumulation and 6-hydroxytryptamine was demonstrated by histofluorescence to be taken up into the myenteric plexus after chemical sympathectomy with 6-hydroxydopamine. Serotonin accumulation was also inhibited by tricyclic antidepressants and amphetamines. The inhibition of serotonin accumulation by these compounds differed from their inhibition of  accumulation of norepinephrine. As in the central nervous system, chlorimipramine was the most potent tricyclic antidepressant against accumulation of serotonin while desmethylimipramine was the most potent inhibitor of the accumulation of norepinephrine. Amphetamines were more effective inhibitors of serotonin accumulation than were tricyclic antidepressants, but all of these drugs were more effective against the accumulation of norepinephrine than serotonin. This study confirms the existence of a unique population of axons int he mammalian myenteric plexus which are distinguishable by their characteristic accumulation of serotonin and have not been found elsewhere in the peripheral nervous system.", "PMID": 978458}
{"id": "pubmed23n0032_11723", "title": "Functional evaluation of 6-hydroxydopamine-induced sympathectomy in the liver of the cat.", "content": "Intraportal injection of 6-hydroxydopamine (25 mg/kg) results in nearly complete functional sympathectomy in the cat liver 1 week after the injection. Functional sympathectomy was verified by comparing the changes in liver blood volume (measured in vivo in a plethysmograph) resulting from direct stimulation of the hepatic nerves in control and pretreated animals. Pretreatment prevented the large reductions in hepatic blood volume normally seen with nerve stimulation while the response to infused noradrenaline was not reduced. Lack of morphological changes in right atrial nerve endings and an unaltered pressor response to bilateral carotid arterial occlusion indicate that sympathectomy at sites other than the liver did not occur.", "contents": "Functional evaluation of 6-hydroxydopamine-induced sympathectomy in the liver of the cat. Intraportal injection of 6-hydroxydopamine (25 mg/kg) results in nearly complete functional sympathectomy in the cat liver 1 week after the injection. Functional sympathectomy was verified by comparing the changes in liver blood volume (measured in vivo in a plethysmograph) resulting from direct stimulation of the hepatic nerves in control and pretreated animals. Pretreatment prevented the large reductions in hepatic blood volume normally seen with nerve stimulation while the response to infused noradrenaline was not reduced. Lack of morphological changes in right atrial nerve endings and an unaltered pressor response to bilateral carotid arterial occlusion indicate that sympathectomy at sites other than the liver did not occur.", "PMID": 978459}
{"id": "pubmed23n0032_11724", "title": "In vitro denervation of the portal vein and caudal artery of the rat.", "content": "The effects of 6-hydroxydopamine (6-OHDA) on the isolated portal vein and caudal artery of the rat were studied to investigate the possibility of producing in vitro adrenergic denervation of these blood vessels. Loss of nerve function was determined by field stimulation of the nerves, using short pulses, and by 3H-norepinephrine (NE) uptake. Addition of 6-OHDA produced contractions of both veins and arteries. Two hours after treatment with 6-OHDA, the contractile responses of the caudal arteries and portal veins to field stimulation were reduced to undetectable levels. At this point, the vessels were unable to take up 3H-NE and incubation of the preparations with l-NE failed to restore the contractile responses to nerve stimulation. Prejunctional supersensitivity to l-NE was observed in the portal vein after 6-OHDA but hot in helically cut strips of caudal artery. Prejunctional supersensitivity of the caudal artery to NE was seen, however, if the vessel geometry was kept intact, suggesting that the uptake mechanism for catecholamines only plays a major role in the termination of action of exgenous NE when norepinephrine is applied through the nerve plexus. We conclude that in vitro treatment with 6-OHDA rapidly produces functional adrenergic denervation of both portal vein and caudal artery of the rat and provides an accurate assessment of the importance of the neuronal uptake mechanism to NE sensitivity.", "contents": "In vitro denervation of the portal vein and caudal artery of the rat. The effects of 6-hydroxydopamine (6-OHDA) on the isolated portal vein and caudal artery of the rat were studied to investigate the possibility of producing in vitro adrenergic denervation of these blood vessels. Loss of nerve function was determined by field stimulation of the nerves, using short pulses, and by 3H-norepinephrine (NE) uptake. Addition of 6-OHDA produced contractions of both veins and arteries. Two hours after treatment with 6-OHDA, the contractile responses of the caudal arteries and portal veins to field stimulation were reduced to undetectable levels. At this point, the vessels were unable to take up 3H-NE and incubation of the preparations with l-NE failed to restore the contractile responses to nerve stimulation. Prejunctional supersensitivity to l-NE was observed in the portal vein after 6-OHDA but hot in helically cut strips of caudal artery. Prejunctional supersensitivity of the caudal artery to NE was seen, however, if the vessel geometry was kept intact, suggesting that the uptake mechanism for catecholamines only plays a major role in the termination of action of exgenous NE when norepinephrine is applied through the nerve plexus. We conclude that in vitro treatment with 6-OHDA rapidly produces functional adrenergic denervation of both portal vein and caudal artery of the rat and provides an accurate assessment of the importance of the neuronal uptake mechanism to NE sensitivity.", "PMID": 978460}
{"id": "pubmed23n0032_11725", "title": "Adrenergic neuronal degeneration induced in portal vein and caudal artery by 6-hydroxydopamine in vitro.", "content": "Fluorescence histochemistry and electron microscopy were used to study the structural consequences of in vitro exposure to the sympatholytic agent 6-hydroxydopamine on two blood vessels, the portal mesenteric vein and caudal artery of the rat. The results showed depletion of catecholamines to indetectable levels associated with clear signs of adrenergic nerve degeneration, such as cytoplasmic shrinking, virtual absence of dense core vesicles and swelling of mitochondria. All of the changes observed occurred within 2 hours in the caudal arteries and 3.5 hours in the portal veins. Comparison of electron and fluorescence micrographs of incubated control specimens with those of unincubated, fresh specimens showed that the nerve endings of the incubated controls were well preserved for at least 3.5 hours. With destruction of nerve endings in such a short period of time, the processes of specific neuronal degeneration could be clearly demonstrated in isolated blood vessels.", "contents": "Adrenergic neuronal degeneration induced in portal vein and caudal artery by 6-hydroxydopamine in vitro. Fluorescence histochemistry and electron microscopy were used to study the structural consequences of in vitro exposure to the sympatholytic agent 6-hydroxydopamine on two blood vessels, the portal mesenteric vein and caudal artery of the rat. The results showed depletion of catecholamines to indetectable levels associated with clear signs of adrenergic nerve degeneration, such as cytoplasmic shrinking, virtual absence of dense core vesicles and swelling of mitochondria. All of the changes observed occurred within 2 hours in the caudal arteries and 3.5 hours in the portal veins. Comparison of electron and fluorescence micrographs of incubated control specimens with those of unincubated, fresh specimens showed that the nerve endings of the incubated controls were well preserved for at least 3.5 hours. With destruction of nerve endings in such a short period of time, the processes of specific neuronal degeneration could be clearly demonstrated in isolated blood vessels.", "PMID": 978461}
{"id": "pubmed23n0032_11726", "title": "Influence of neonatal and adult hyperthyroidism on behavior and biosynthetic capacity for norepinephrine, dopamine and 5-hydroxytryptamine in rat brain.", "content": "In neonatal rats, administration of l-triiodothyronine (10 mug/100 g/day) for 30 days presented signs of hyperthyroidism which included accelerated development of a variety of physical and behavioral characteristics accompanying maturation. The spontaneous motor activity was increased by 69%. Exposure of developing rats to thyroid hormone significantly increased the endogenous concentration of striatal tyrosine and the activity of tyrosine hydroxylase as well as the levels of dopamine in several brain regions. The concentration of striatal homovanillic acid and 3,4-dihydroxyphenylacetic acid, the chief metabolites of dopamine, was also increased and the magnitude of change was greater than the rise in dopamine. Despite increases in the activity of tyrosine hydroxylase and the availability of the substrate tyrosine, the steady-state levels of norepinephrine remained unaltered in various regions of brain except in cerebellum. Futhermore, neonatal hyperthyroidism significantly increased the levels of midbrain tryptophan and tryptophan hydroxylase activity but produced no change in 5-hydroxytryptamine levels of several discrete brain regions, except hypothalamus and cerebellum where its concentration was slightly decreased. However, the 5-hydroxyindoleacetic acid levels were enhanced in hypothalamus, ponsmedulla, midbrain, striatum and hippocampus. The elevated levels of 5-hydroxyindoleacetic acid did not seem to be due to increased intraneuronal deamination of 5-hydroxytryptamine since monoamine oxidase activity was not affected in cerebral cortex and midbrain of hyperthyroid rats. The data demonstrate that hyperthyroidism significantly increased the synthesis as well as the utilization of catecholamines and 5-hydroxytryptamine in maturing brain. Since the mature brain is known to respond differently to thyroid hormone action than does the developing brain, the effect of L-triiodothyronine treatment on various putative neurohumors also was examined in adult rats. Whereas administration of l-triiodothyronine (10 mug/100 g/day) for 30 days to 120-day-old rats increased the levels of tyrosine by 23% and of tryptophan by 43%, no appreciable change was noted in tryptophan hydroxylase activity. In contrast to neonatal hyperthyroidism, excess of thyroid hormone in adult rats failed to produce any change in motor activity and tended to decrease striatal tyrosine hydroxylase activity only slightly. The concentration of dopamine remained unchanged in all regions of the brain except in midbrain where it rose by 19%. Whereas norepinephrine concentration was altered in hypothalamus, pons-medulla and midbrain, the levels of 5-hydroxytryptamine and its metabolite, 5-hydroxyindoleacetic acid, were significantly decreased in striatum and cerebellum. Since dopaminergic and noradrenergic neurons are the critical components of the motor system, the possibility exists that elevated behavioral activity in young L-triiodothyronine-treated animals might be associated with increased turnover of catecholamines in neuronal tissue.", "contents": "Influence of neonatal and adult hyperthyroidism on behavior and biosynthetic capacity for norepinephrine, dopamine and 5-hydroxytryptamine in rat brain. In neonatal rats, administration of l-triiodothyronine (10 mug/100 g/day) for 30 days presented signs of hyperthyroidism which included accelerated development of a variety of physical and behavioral characteristics accompanying maturation. The spontaneous motor activity was increased by 69%. Exposure of developing rats to thyroid hormone significantly increased the endogenous concentration of striatal tyrosine and the activity of tyrosine hydroxylase as well as the levels of dopamine in several brain regions. The concentration of striatal homovanillic acid and 3,4-dihydroxyphenylacetic acid, the chief metabolites of dopamine, was also increased and the magnitude of change was greater than the rise in dopamine. Despite increases in the activity of tyrosine hydroxylase and the availability of the substrate tyrosine, the steady-state levels of norepinephrine remained unaltered in various regions of brain except in cerebellum. Futhermore, neonatal hyperthyroidism significantly increased the levels of midbrain tryptophan and tryptophan hydroxylase activity but produced no change in 5-hydroxytryptamine levels of several discrete brain regions, except hypothalamus and cerebellum where its concentration was slightly decreased. However, the 5-hydroxyindoleacetic acid levels were enhanced in hypothalamus, ponsmedulla, midbrain, striatum and hippocampus. The elevated levels of 5-hydroxyindoleacetic acid did not seem to be due to increased intraneuronal deamination of 5-hydroxytryptamine since monoamine oxidase activity was not affected in cerebral cortex and midbrain of hyperthyroid rats. The data demonstrate that hyperthyroidism significantly increased the synthesis as well as the utilization of catecholamines and 5-hydroxytryptamine in maturing brain. Since the mature brain is known to respond differently to thyroid hormone action than does the developing brain, the effect of L-triiodothyronine treatment on various putative neurohumors also was examined in adult rats. Whereas administration of l-triiodothyronine (10 mug/100 g/day) for 30 days to 120-day-old rats increased the levels of tyrosine by 23% and of tryptophan by 43%, no appreciable change was noted in tryptophan hydroxylase activity. In contrast to neonatal hyperthyroidism, excess of thyroid hormone in adult rats failed to produce any change in motor activity and tended to decrease striatal tyrosine hydroxylase activity only slightly. The concentration of dopamine remained unchanged in all regions of the brain except in midbrain where it rose by 19%. Whereas norepinephrine concentration was altered in hypothalamus, pons-medulla and midbrain, the levels of 5-hydroxytryptamine and its metabolite, 5-hydroxyindoleacetic acid, were significantly decreased in striatum and cerebellum. Since dopaminergic and noradrenergic neurons are the critical components of the motor system, the possibility exists that elevated behavioral activity in young L-triiodothyronine-treated animals might be associated with increased turnover of catecholamines in neuronal tissue.", "PMID": 978462}
{"id": "pubmed23n0032_11727", "title": "Tryptoline inhibition of serotonin uptake in rat forebrain homogenates.", "content": "We have examined the effects of six tryptolines (tetrahydro-beta-carbolines) upon 5-hydroxytryptamine (5-HT) uptake into rat forebrain homogenates. All six were found to be competitive inhibitors of 5-HT uptake. 5-Hydroxytryptoline, the most potent inhibitor, had an inhibitor constant (Ki) of 0.3 mugM. Both 5-hydroxytryptoline and 5-hydroxymethtryptoline were also shown to be relatively selective against 5-HT uptake, being 20 times less potent against norepinephrine uptake and 40 times less potent against dopamine. Since it is possible that tryptolines might be formed as a result of alcohol consumption, these results suggest that it is important to examine the possibilities that such compounds mediate some of the effects of alcohol upon serotonergic pathways.", "contents": "Tryptoline inhibition of serotonin uptake in rat forebrain homogenates. We have examined the effects of six tryptolines (tetrahydro-beta-carbolines) upon 5-hydroxytryptamine (5-HT) uptake into rat forebrain homogenates. All six were found to be competitive inhibitors of 5-HT uptake. 5-Hydroxytryptoline, the most potent inhibitor, had an inhibitor constant (Ki) of 0.3 mugM. Both 5-hydroxytryptoline and 5-hydroxymethtryptoline were also shown to be relatively selective against 5-HT uptake, being 20 times less potent against norepinephrine uptake and 40 times less potent against dopamine. Since it is possible that tryptolines might be formed as a result of alcohol consumption, these results suggest that it is important to examine the possibilities that such compounds mediate some of the effects of alcohol upon serotonergic pathways.", "PMID": 978463}
{"id": "pubmed23n0032_11728", "title": "The existence of tolerance to and cross-tolerance between d-amphetamine and methylphenidate for their effects on milk consumption and on differential-reinforcement-of-low-rate performance in the rat.", "content": "Administration of d-amphetamine (2.5 mg/kg) or methylphenidate (20 mg/kg) decreased milk consumption in rats. When these drugs were administered daily, tolerance developed to this effect over the course of 20 days. Cross-tolerance to the effects on milk consumption occurred between d-amphetamine and methylphenidate over a 4-fold range of dose of both drugs. Administration of d-amphetamine (1.5 mg/kg) or methylphenidate (10 mg/kg) disrupted responding under differential-reinforcement-or-low-rate (DRL) contingencies. Both drugs increased response rate, decreased frequency of reinforcement and shifted the mode of the inter-response time distribution to the left. When the drugs were administered daily, tolerance developed to all these effects over the course of 20 days. Cross-tolerance to the effects on DRL behavior occurred between d-amphetamine and methylphenidate over a 4-fold range of dose of both drugs. Daily administration of d-amphetamine (2.5 mg/kg) but not of methylphenidate (20 mg/kg) resulted in decreased norepinephrine (NE) levels in brain; the decreased norepinephrine levels, which occur with repeated d-amphetamine administration, are believed to result from the storage in noradrenergic neurons of p-hydroxynorephedrine, a metabolite of d-amphetamine. Radioactivity was not detectable in brain 24 hours after the last of 15 daily doses of radiolabeled methylphenidate. These results suggest that d-amphetamine, but not methylphenidate, is metabolized to a compound which is stored in noradrenergic neurons. The existence of behavioral cross-tolerance between d-amphetamine and methylphenidate is therefore inconsistent with the hypothesis that tolerance to the behavioral effects of d-amphetamine is due to the metabolism of d-amphetamine to p-hydroxynorephedrine, a false transmitter in noradrenergic neurons.", "contents": "The existence of tolerance to and cross-tolerance between d-amphetamine and methylphenidate for their effects on milk consumption and on differential-reinforcement-of-low-rate performance in the rat. Administration of d-amphetamine (2.5 mg/kg) or methylphenidate (20 mg/kg) decreased milk consumption in rats. When these drugs were administered daily, tolerance developed to this effect over the course of 20 days. Cross-tolerance to the effects on milk consumption occurred between d-amphetamine and methylphenidate over a 4-fold range of dose of both drugs. Administration of d-amphetamine (1.5 mg/kg) or methylphenidate (10 mg/kg) disrupted responding under differential-reinforcement-or-low-rate (DRL) contingencies. Both drugs increased response rate, decreased frequency of reinforcement and shifted the mode of the inter-response time distribution to the left. When the drugs were administered daily, tolerance developed to all these effects over the course of 20 days. Cross-tolerance to the effects on DRL behavior occurred between d-amphetamine and methylphenidate over a 4-fold range of dose of both drugs. Daily administration of d-amphetamine (2.5 mg/kg) but not of methylphenidate (20 mg/kg) resulted in decreased norepinephrine (NE) levels in brain; the decreased norepinephrine levels, which occur with repeated d-amphetamine administration, are believed to result from the storage in noradrenergic neurons of p-hydroxynorephedrine, a metabolite of d-amphetamine. Radioactivity was not detectable in brain 24 hours after the last of 15 daily doses of radiolabeled methylphenidate. These results suggest that d-amphetamine, but not methylphenidate, is metabolized to a compound which is stored in noradrenergic neurons. The existence of behavioral cross-tolerance between d-amphetamine and methylphenidate is therefore inconsistent with the hypothesis that tolerance to the behavioral effects of d-amphetamine is due to the metabolism of d-amphetamine to p-hydroxynorephedrine, a false transmitter in noradrenergic neurons.", "PMID": 978464}
{"id": "pubmed23n0032_11729", "title": "Studies on the effect of 5,5'-diphenylhydantoin on in vitro protein synthesis in rat brain.", "content": "Previous investigators have reported binding of the drug 5,5'-diphenylhydantoin (DPH) to RNA and DNA and an effect of this compound on protein synthesis. We have studied the effect of DPH on in vitro protein synthesis by investigating the possible role of this drug on poly-Uracil-directed polyphenylalanine synthesis and natural mRNA-directed amino acid incorporation into polypeptides. There was no demonstrable effect of DPH on either of these reactions. It was also found the DPH did not alter the rate of aminoacylation of purified rat liver tRNAs. The in vivo daily administration of DPH to animals for a period of 2 weeks did not appear to affect the in vitro poly-Uracil-directed polyphenylalanine synthesis (chain elongation aspects of protein synthesis) in adult rat brain. It was also found that DPH did not inhibit DNA-dependent RNA synthesis as catalyzed by RNA polymerase. These studies do not support the hypothesis that DPH plays a role in protein synthesis in adult brain cells.", "contents": "Studies on the effect of 5,5'-diphenylhydantoin on in vitro protein synthesis in rat brain. Previous investigators have reported binding of the drug 5,5'-diphenylhydantoin (DPH) to RNA and DNA and an effect of this compound on protein synthesis. We have studied the effect of DPH on in vitro protein synthesis by investigating the possible role of this drug on poly-Uracil-directed polyphenylalanine synthesis and natural mRNA-directed amino acid incorporation into polypeptides. There was no demonstrable effect of DPH on either of these reactions. It was also found the DPH did not alter the rate of aminoacylation of purified rat liver tRNAs. The in vivo daily administration of DPH to animals for a period of 2 weeks did not appear to affect the in vitro poly-Uracil-directed polyphenylalanine synthesis (chain elongation aspects of protein synthesis) in adult rat brain. It was also found that DPH did not inhibit DNA-dependent RNA synthesis as catalyzed by RNA polymerase. These studies do not support the hypothesis that DPH plays a role in protein synthesis in adult brain cells.", "PMID": 978465}
{"id": "pubmed23n0032_11730", "title": "Anticonvulsant action of acute morphine administration in rats.", "content": "The effect of acute administration of morphine on cerebral excitability was investigated in rats with two convulsant drugs: flurothyl (hexafluorodiethyl ether) and pentylenetetrazol (PTZ). In the flurothyl study, adult male Sprague-Dawley (S-D) rats were injected subcutaneously with morphine sulfate in doses ranging from 0.5 to 256 mg/kg. At 15, 30, 60 and 120 minutes after morphine injection, flurothyl was administered by inhalation and the seizure thresholds were determined. In the PTZ study, 64 mg/kg of morphine sulfate were injected subcutaneously into both S-D and CFN (Wistar-derived) rats. Thresholds to PTZ seizures were measured after administering the convulsant either by the intraperitoneal or intravenous route. The data revealed an anticonvulsant action of morphine on both flurothyl and PTZ. Peak time for this effect on flurothyl seizures was 30 minutes after subcutaneous administration of the opiate, with the maximal anticonvulsant activity appearing at the 64-mg/kg dose. The increase in seizure threshold in S-D rats at this dose was 36% with flurothyl, 94% with intravenous PTZ and 352% with i.p. PTZ. Morphine had a less dramatic influence on raising the latter seizure threshold in the CFN than in the S-D strain. The graded dose-related anticonvulsant action is independent of the respiratory depression associated with morphine administration and appears to be a reflection of an altered central nervous system excitability produced by the narcotic in rats.", "contents": "Anticonvulsant action of acute morphine administration in rats. The effect of acute administration of morphine on cerebral excitability was investigated in rats with two convulsant drugs: flurothyl (hexafluorodiethyl ether) and pentylenetetrazol (PTZ). In the flurothyl study, adult male Sprague-Dawley (S-D) rats were injected subcutaneously with morphine sulfate in doses ranging from 0.5 to 256 mg/kg. At 15, 30, 60 and 120 minutes after morphine injection, flurothyl was administered by inhalation and the seizure thresholds were determined. In the PTZ study, 64 mg/kg of morphine sulfate were injected subcutaneously into both S-D and CFN (Wistar-derived) rats. Thresholds to PTZ seizures were measured after administering the convulsant either by the intraperitoneal or intravenous route. The data revealed an anticonvulsant action of morphine on both flurothyl and PTZ. Peak time for this effect on flurothyl seizures was 30 minutes after subcutaneous administration of the opiate, with the maximal anticonvulsant activity appearing at the 64-mg/kg dose. The increase in seizure threshold in S-D rats at this dose was 36% with flurothyl, 94% with intravenous PTZ and 352% with i.p. PTZ. Morphine had a less dramatic influence on raising the latter seizure threshold in the CFN than in the S-D strain. The graded dose-related anticonvulsant action is independent of the respiratory depression associated with morphine administration and appears to be a reflection of an altered central nervous system excitability produced by the narcotic in rats.", "PMID": 978466}
{"id": "pubmed23n0032_11731", "title": "Action of anaphylaxis and histamine on isolated mammalian ventricles: contractile hyperactivation and changes of membrane potentials.", "content": "The effects of anaphylaxis and histamine on the action potential and the isometric contraction of isolated guinea-pig papillary muscles have been investigated. The anaphylactic reaction was found to affect directly ventricular muscle fibers. The anaphylactic alterations of membrane and contraction parameters were very similar to effects of histamine and were not affected by pretreatment with reserpine. Isometric contraction developed typical features of contractile hyperactivation, characterized by a marked increase of peak force associated with shortening of time to peak of contraction and the appearance of oscillatory contractions and aftercontractions. Mechanical alternans and electrical alternans were also observed. Oscillatory contractions occurring during membrane depolarization were increased with prolongation of the action potential duration. After contractions developed after complete repolarization of the membrane and increased at higher stimulation rates. The anaphylactic reaction and histamine caused shortening of the action potential. Resting potential, overshoot and maximum rate of rise of action potential were not significantly changed. A common mechanism of contractile hyperactivation based on a multicompartment model of the intracellular Ca movements in discussed.", "contents": "Action of anaphylaxis and histamine on isolated mammalian ventricles: contractile hyperactivation and changes of membrane potentials. The effects of anaphylaxis and histamine on the action potential and the isometric contraction of isolated guinea-pig papillary muscles have been investigated. The anaphylactic reaction was found to affect directly ventricular muscle fibers. The anaphylactic alterations of membrane and contraction parameters were very similar to effects of histamine and were not affected by pretreatment with reserpine. Isometric contraction developed typical features of contractile hyperactivation, characterized by a marked increase of peak force associated with shortening of time to peak of contraction and the appearance of oscillatory contractions and aftercontractions. Mechanical alternans and electrical alternans were also observed. Oscillatory contractions occurring during membrane depolarization were increased with prolongation of the action potential duration. After contractions developed after complete repolarization of the membrane and increased at higher stimulation rates. The anaphylactic reaction and histamine caused shortening of the action potential. Resting potential, overshoot and maximum rate of rise of action potential were not significantly changed. A common mechanism of contractile hyperactivation based on a multicompartment model of the intracellular Ca movements in discussed.", "PMID": 978467}
{"id": "pubmed23n0032_11732", "title": "Influence of calcium concentration on the rate of onset of cardiac glycoside and aglycone inotropism.", "content": "Isolated rabbit atria were employed to examine the rate of onset of inotropism of two glycosides (strophanthin and ouabain) and aglycones (acetylstrophanthidin and ouabagenin) in the presence of various concentrations of calcium (0.55, 1.1, 2.2, 4.4 and 8.8 mM). The rate of onset of glycoside-induced inotropism was found to be greatly influenced by the calcium concentration. The rate of onset was increased in both low and high calcium, with the minimum rate of onset at 1.1 or 2.2 mM calcium. In contrast, the rate of onset of aglycone-induced inotropism was not dependent on calcium between 0.55 and 4.4 mM calcium. The rate of onset of aglycone-induced inotropism was increased at 8.8 mM calcium. The results are interpreted by postulating that the digitalis inotropic receptor is located in a membrane-limited compartment to which aglycones may gain access via passive diffusion but to which glycosides gain access mainly via a (calcium-dependent) carrier mechanism.", "contents": "Influence of calcium concentration on the rate of onset of cardiac glycoside and aglycone inotropism. Isolated rabbit atria were employed to examine the rate of onset of inotropism of two glycosides (strophanthin and ouabain) and aglycones (acetylstrophanthidin and ouabagenin) in the presence of various concentrations of calcium (0.55, 1.1, 2.2, 4.4 and 8.8 mM). The rate of onset of glycoside-induced inotropism was found to be greatly influenced by the calcium concentration. The rate of onset was increased in both low and high calcium, with the minimum rate of onset at 1.1 or 2.2 mM calcium. In contrast, the rate of onset of aglycone-induced inotropism was not dependent on calcium between 0.55 and 4.4 mM calcium. The rate of onset of aglycone-induced inotropism was increased at 8.8 mM calcium. The results are interpreted by postulating that the digitalis inotropic receptor is located in a membrane-limited compartment to which aglycones may gain access via passive diffusion but to which glycosides gain access mainly via a (calcium-dependent) carrier mechanism.", "PMID": 978468}
{"id": "pubmed23n0032_11733", "title": "A comparison of effects of SKF 525-A and procaine on excitation-contraction coupling in single crayfish muscle fibers.", "content": "The effects of beta-diethylaminoethyldiphenylpropylacetate hydrochloride (SKF 525-A) on excitation-contraction coupling and Ca-dependent electrogenesis are compared to those of procaine. At pH 7.2, SKF 525-A and procaine occur essentially (greater that 97%) as a free base and as a cation, respectively. At this pH, SKF 525-A elicited tension development, blocked K-induced contractures and the K-induced repriming of caffeine contractions, potentiated caffeine-induced tensions, inhibited the procaine-induced spikes and twitches and, depending on the concentration, either potentiated (25-50 muM) or depressed (greater than 100 muM) the tensions associated with the graded membrane electrogenesis. At the same pH, procaine blocked the contractions elicited by SKF 525-A, by high K media, by the graded electrogenesis and by caffeine, and converted the graded membrane responses into all-or-none spikes. It is proposed that SKF 525-A as a free base 1) inhibits membrane Ca activation more effectively than it depresses K conductance and 2) is synergistic with caffeine in reducing the effectiveness of Ca sequestration by the sarcoplasmic reticulum. Procaine as a cationic molecule is thought to depress K activation more than Ca activation during depolarization and to block the release of Ca ions from the sarcoplasmic reticulum.", "contents": "A comparison of effects of SKF 525-A and procaine on excitation-contraction coupling in single crayfish muscle fibers. The effects of beta-diethylaminoethyldiphenylpropylacetate hydrochloride (SKF 525-A) on excitation-contraction coupling and Ca-dependent electrogenesis are compared to those of procaine. At pH 7.2, SKF 525-A and procaine occur essentially (greater that 97%) as a free base and as a cation, respectively. At this pH, SKF 525-A elicited tension development, blocked K-induced contractures and the K-induced repriming of caffeine contractions, potentiated caffeine-induced tensions, inhibited the procaine-induced spikes and twitches and, depending on the concentration, either potentiated (25-50 muM) or depressed (greater than 100 muM) the tensions associated with the graded membrane electrogenesis. At the same pH, procaine blocked the contractions elicited by SKF 525-A, by high K media, by the graded electrogenesis and by caffeine, and converted the graded membrane responses into all-or-none spikes. It is proposed that SKF 525-A as a free base 1) inhibits membrane Ca activation more effectively than it depresses K conductance and 2) is synergistic with caffeine in reducing the effectiveness of Ca sequestration by the sarcoplasmic reticulum. Procaine as a cationic molecule is thought to depress K activation more than Ca activation during depolarization and to block the release of Ca ions from the sarcoplasmic reticulum.", "PMID": 978469}
{"id": "pubmed23n0032_11734", "title": "Mechanism of action of spironolactone on adrenocortical function in guinea pigs.", "content": "Spironolactone administration (50 mg/kg/day for 3 days) to make guinea pigs decreased cortisol production by adrenal slices in vitro. Adrenal microsomal and mitochondrial cytochrome P-450 levels were also decreased after treatment with spironolactone. The decline in adrenal cytochrome P-450 content was accompanied by decreases in microsomal 21-hydroxylase and mitochondrial cholesterol side-chain cleavage and 11beta-hydroxylase activities. Activities of other adrenal enzymes, such as delta4-hydrogenase and NADPH-cytochrome c reductase, were unaffected by spironolactone treatment. Cortisone administration to guinea pigs failed to mimic the effects of spironolactone on adrenal function, which indicates specificity of spironolactone action and excludes inhibition of adrenocorticotropin secretion as a mode of action. Addition of spironolactone to isolated adrenal mitochondria or microsomes produced type I spectral changes with spectral dissociation constants similar to those for endogenous steroid substrates. Spironolactone, in vitro, inhibited 11beta- but not 21-hydroxylase activity. The results indicate that spironolactone administration diminishes the activity of adrenal mitochondrial as well as microsomal cytochrome P-450-containing enzymes, resulting in a fall in corticosteroid output.", "contents": "Mechanism of action of spironolactone on adrenocortical function in guinea pigs. Spironolactone administration (50 mg/kg/day for 3 days) to make guinea pigs decreased cortisol production by adrenal slices in vitro. Adrenal microsomal and mitochondrial cytochrome P-450 levels were also decreased after treatment with spironolactone. The decline in adrenal cytochrome P-450 content was accompanied by decreases in microsomal 21-hydroxylase and mitochondrial cholesterol side-chain cleavage and 11beta-hydroxylase activities. Activities of other adrenal enzymes, such as delta4-hydrogenase and NADPH-cytochrome c reductase, were unaffected by spironolactone treatment. Cortisone administration to guinea pigs failed to mimic the effects of spironolactone on adrenal function, which indicates specificity of spironolactone action and excludes inhibition of adrenocorticotropin secretion as a mode of action. Addition of spironolactone to isolated adrenal mitochondria or microsomes produced type I spectral changes with spectral dissociation constants similar to those for endogenous steroid substrates. Spironolactone, in vitro, inhibited 11beta- but not 21-hydroxylase activity. The results indicate that spironolactone administration diminishes the activity of adrenal mitochondrial as well as microsomal cytochrome P-450-containing enzymes, resulting in a fall in corticosteroid output.", "PMID": 978470}
{"id": "pubmed23n0032_11735", "title": "Metabolism of prostaglandins A and E in the perfused rabbit lung and the effects of selected inhibitors.", "content": "Hypothermia (4 degrees C) reversibly inhibits metabolism of prostaglandin A1 (PGA1) in the perfused rabbit lung and decreases the transit time of PGA1 through the lung. Co-perfusion of PGA1 (0.28 muM) and PGE1 (2.8 muM) resulted in 48% inhibition of PGA1 metabolism. Ouabain and phenoxybenzamine (10(-5) M) did not significantly affect PGA1 metabolism. We examined the effect of diphloretin phosphate (DPP; 6.0 mu/ml) on the metabolism of prostaglandin A1 (0.28-5.03 muM) and E1 (PGE1;0.28-11.56 muM). The metabolism of both prostaglandins appeared to be saturable processes and, in the case of PGE, the data conformed to Michaelis-Menten kinetics: the apparent Km (muM) and apparent Vmax (nmol/lung X min-1) in control lungs were 9.0 +/- 0.3 and 87.9 +/- 1.4, respectively, and in the DPP-treated lungs were 9.6 +/- 0.5 and 57.7 +/- 1.8. This suggests that DPP acts in a noncompetitive manner. The magnitude of inhibition of PGA1 and PGE1 metabolism (both at 0.28 muM) was linearly related to the DPP concentration, over the range of 0.06 to 25.0 mug/ml. The ID50 values of DPP inhibition of PGA1 and PGE1 metabolism were 2.2 and 8.4 mug/ml, respectively. Perfusion of PGA1 at 2.96 muM or higher concentrations caused reversible vasoconstriction which was significantly inhibited (P less than .05) by DPP (6.0 mug/ml) by an average of 77.2 +/- 5.8% (n = 7).", "contents": "Metabolism of prostaglandins A and E in the perfused rabbit lung and the effects of selected inhibitors. Hypothermia (4 degrees C) reversibly inhibits metabolism of prostaglandin A1 (PGA1) in the perfused rabbit lung and decreases the transit time of PGA1 through the lung. Co-perfusion of PGA1 (0.28 muM) and PGE1 (2.8 muM) resulted in 48% inhibition of PGA1 metabolism. Ouabain and phenoxybenzamine (10(-5) M) did not significantly affect PGA1 metabolism. We examined the effect of diphloretin phosphate (DPP; 6.0 mu/ml) on the metabolism of prostaglandin A1 (0.28-5.03 muM) and E1 (PGE1;0.28-11.56 muM). The metabolism of both prostaglandins appeared to be saturable processes and, in the case of PGE, the data conformed to Michaelis-Menten kinetics: the apparent Km (muM) and apparent Vmax (nmol/lung X min-1) in control lungs were 9.0 +/- 0.3 and 87.9 +/- 1.4, respectively, and in the DPP-treated lungs were 9.6 +/- 0.5 and 57.7 +/- 1.8. This suggests that DPP acts in a noncompetitive manner. The magnitude of inhibition of PGA1 and PGE1 metabolism (both at 0.28 muM) was linearly related to the DPP concentration, over the range of 0.06 to 25.0 mug/ml. The ID50 values of DPP inhibition of PGA1 and PGE1 metabolism were 2.2 and 8.4 mug/ml, respectively. Perfusion of PGA1 at 2.96 muM or higher concentrations caused reversible vasoconstriction which was significantly inhibited (P less than .05) by DPP (6.0 mug/ml) by an average of 77.2 +/- 5.8% (n = 7).", "PMID": 978471}
{"id": "pubmed23n0032_11736", "title": "Analgesic nephropathy: renal distribution of acetaminophen and its conjugates.", "content": "Acetaminophen (APAP) and its conjugates (APAP-conj) are the major metabolites of phenacetin. To study their significance in the pathogenesis of analgesic nephropathy, their concentrations in the renal parenchyma were determined in conjunction with clearance measurements in 15 dogs with inulin as a reference marker. Particular attention was given to interpretation of the mechanisms responsible for the concentrations generated within the papilla. For APAP, APAP-conj and inulin there is a significant corticomedullary gradient which is inversely related to the rate of urine flow. For inulin and APAP-conj, this gradient may be explained by their concentrations within the tubular fluid of the distal nephron. With concentration in the urine as a reference, the apparent volume of distribution of inulin within the papilla is not fixed, but varies with the rate of urine flow. Using several different types of comparison with inulin, we found that the results with APAP are consistent with its penetration by diffusion into the cellular compartment of the papilla. Dehydration leads to maximal concentrations of APAP in the urine as well as in the tubular fluid and the cells of the distal nephron. This is consistent with the kinetics of diffusion.", "contents": "Analgesic nephropathy: renal distribution of acetaminophen and its conjugates. Acetaminophen (APAP) and its conjugates (APAP-conj) are the major metabolites of phenacetin. To study their significance in the pathogenesis of analgesic nephropathy, their concentrations in the renal parenchyma were determined in conjunction with clearance measurements in 15 dogs with inulin as a reference marker. Particular attention was given to interpretation of the mechanisms responsible for the concentrations generated within the papilla. For APAP, APAP-conj and inulin there is a significant corticomedullary gradient which is inversely related to the rate of urine flow. For inulin and APAP-conj, this gradient may be explained by their concentrations within the tubular fluid of the distal nephron. With concentration in the urine as a reference, the apparent volume of distribution of inulin within the papilla is not fixed, but varies with the rate of urine flow. Using several different types of comparison with inulin, we found that the results with APAP are consistent with its penetration by diffusion into the cellular compartment of the papilla. Dehydration leads to maximal concentrations of APAP in the urine as well as in the tubular fluid and the cells of the distal nephron. This is consistent with the kinetics of diffusion.", "PMID": 978472}
{"id": "pubmed23n0032_11737", "title": "Tissue distribution of levo-methadone in nonpregnant and pregnant female and male mice: effect of SKF 525-A 1,2.", "content": "Male, nonpregnant and pregnant female (15-17 gestational days) mice were injected i.p. with saline or SKF 525-A (50 mg/kg) and 1 hour later with levo-3H-1-methadone (205 mug/kg; 0.8 and 5 mg/kg). Levels of free methadone were examined in some or all of the following tissues: brain, plasma, liver, lung, spleen, kidney, heart, eye, placenta, amniotic fluid, whole fetus, fetal brain and liver. In saline controls, the methadone concentrations in several adult tissues, whole fetuses, fetal brain and liver reached peak levels at 15 minutes and were negligible at 24 hours. Tissue levels of methadone increased in a dose-related manner; however, the brain/plasma concentration ratios with the three doses were almost unity at 15 minutes, 1 hour and 3 hours. Among adult tissues with high concentrations were the lung, liver, kidney and spleen; the brain in contrast contained very low concentrations. No notable differences were detected in the tissue levels of methadone in males and nonpregnant females; however, in pregnant mice significantly higher levels were observed in the lung and the liver. Fetal brain, liver and whole fetus concentrations were approximately similar. At 15 minutes, the fetal brain contained about 3 times more methadone than the maternal brain and the difference increased at subsequent times. Nonpregnant females excreted somewhat larger amounts of 3H in the urine than did pregnant females. SKF 525-A markedly enhanced and prolonged the concentrations of methadone in all the adult and the fetal tissues and lowered urinary excretion of total radioactivity in nonpregnant and pregnant females. Marked increases in tissue radioactivity after SKF 525-A might be due to a combination of effects involving inhibition of drug metabolism, altered tissue distribution and lower urinary excretion.", "contents": "Tissue distribution of levo-methadone in nonpregnant and pregnant female and male mice: effect of SKF 525-A 1,2. Male, nonpregnant and pregnant female (15-17 gestational days) mice were injected i.p. with saline or SKF 525-A (50 mg/kg) and 1 hour later with levo-3H-1-methadone (205 mug/kg; 0.8 and 5 mg/kg). Levels of free methadone were examined in some or all of the following tissues: brain, plasma, liver, lung, spleen, kidney, heart, eye, placenta, amniotic fluid, whole fetus, fetal brain and liver. In saline controls, the methadone concentrations in several adult tissues, whole fetuses, fetal brain and liver reached peak levels at 15 minutes and were negligible at 24 hours. Tissue levels of methadone increased in a dose-related manner; however, the brain/plasma concentration ratios with the three doses were almost unity at 15 minutes, 1 hour and 3 hours. Among adult tissues with high concentrations were the lung, liver, kidney and spleen; the brain in contrast contained very low concentrations. No notable differences were detected in the tissue levels of methadone in males and nonpregnant females; however, in pregnant mice significantly higher levels were observed in the lung and the liver. Fetal brain, liver and whole fetus concentrations were approximately similar. At 15 minutes, the fetal brain contained about 3 times more methadone than the maternal brain and the difference increased at subsequent times. Nonpregnant females excreted somewhat larger amounts of 3H in the urine than did pregnant females. SKF 525-A markedly enhanced and prolonged the concentrations of methadone in all the adult and the fetal tissues and lowered urinary excretion of total radioactivity in nonpregnant and pregnant females. Marked increases in tissue radioactivity after SKF 525-A might be due to a combination of effects involving inhibition of drug metabolism, altered tissue distribution and lower urinary excretion.", "PMID": 978473}
{"id": "pubmed23n0032_11738", "title": "Effect of 6-hydroxydopamine and 5,6-dihydroxytryptamine on the response of the coaxially stimulated guinea-pig ileum to morphine.", "content": "Studies were conducted to determine the role of norepinephrine and 5-hydroxytryptamine in the action of morphine in the coaxially stimulated guinea-pig ileum. 6-hydroxydopamine produced supersensitivity to norepinephrine and decreased the levels of norepinephrine in the ileum. 6-Hydroxydopamine did not interfere with the acute effects of morphine but did interfere with the degree of tolerance developed to morphine, which is in contrast to reported results of the effect of 6-hydroxydopamine on the analgesic response to morphine. No evidence was found that 5,6-dihydroxytryptamine altered the acute or chronic response of the ileum to morphine. Again this is in contrast to results for the analgesic receptor where 5,6-dihydroxytryptamine has been reported to inhibit the development of tolerance to morphine. Thus, the role of the biogenic amines in the action of morphine in the ileum appears to differ from their reported role in the action of morphine in the central nervous system.", "contents": "Effect of 6-hydroxydopamine and 5,6-dihydroxytryptamine on the response of the coaxially stimulated guinea-pig ileum to morphine. Studies were conducted to determine the role of norepinephrine and 5-hydroxytryptamine in the action of morphine in the coaxially stimulated guinea-pig ileum. 6-hydroxydopamine produced supersensitivity to norepinephrine and decreased the levels of norepinephrine in the ileum. 6-Hydroxydopamine did not interfere with the acute effects of morphine but did interfere with the degree of tolerance developed to morphine, which is in contrast to reported results of the effect of 6-hydroxydopamine on the analgesic response to morphine. No evidence was found that 5,6-dihydroxytryptamine altered the acute or chronic response of the ileum to morphine. Again this is in contrast to results for the analgesic receptor where 5,6-dihydroxytryptamine has been reported to inhibit the development of tolerance to morphine. Thus, the role of the biogenic amines in the action of morphine in the ileum appears to differ from their reported role in the action of morphine in the central nervous system.", "PMID": 978474}
{"id": "pubmed23n0032_11739", "title": "Effects of neonatal or maternal methadone administration on ornithine decarboxylase activity in brain and heart of developing rats.", "content": "Methadone was administered daily to pregnant or nursing rats, or directly to neonates, and the effects on brain and heart weights and ornithine decarboxylase (ODC) activities were determined during postnatal development. Exposure to methadone in the postnatal period either directly or via the mother resulted in delays in maturational decreases in brain ODC accompanied by deficits in brain weight. Prenatal exposure alone had less effect on brain weight or ODC but appeared to enhance the effect of subsequent methadone exposure during the postnatal period. In the heart, direct methadone exposure or prenatal plus postnatal maternal administration led to a pattern of altered ODC activity consistent with delayed development, accompanied by heart weight deficits. The disturbance of heart ODC development after purely prenatal or purely postnatal maternal exposures differed from that obtained after direct administration to the neonate. These data show that exposure to methadone during fetal and/or neonatal life produces alterations in polyamine metabolism which may result in abnormal organ development. The type of change is dependent upon the period and route of exposure and may reflect both direct effects on the pup and indirect effect from drug-induced alterations in maternal metabolism or behavior.", "contents": "Effects of neonatal or maternal methadone administration on ornithine decarboxylase activity in brain and heart of developing rats. Methadone was administered daily to pregnant or nursing rats, or directly to neonates, and the effects on brain and heart weights and ornithine decarboxylase (ODC) activities were determined during postnatal development. Exposure to methadone in the postnatal period either directly or via the mother resulted in delays in maturational decreases in brain ODC accompanied by deficits in brain weight. Prenatal exposure alone had less effect on brain weight or ODC but appeared to enhance the effect of subsequent methadone exposure during the postnatal period. In the heart, direct methadone exposure or prenatal plus postnatal maternal administration led to a pattern of altered ODC activity consistent with delayed development, accompanied by heart weight deficits. The disturbance of heart ODC development after purely prenatal or purely postnatal maternal exposures differed from that obtained after direct administration to the neonate. These data show that exposure to methadone during fetal and/or neonatal life produces alterations in polyamine metabolism which may result in abnormal organ development. The type of change is dependent upon the period and route of exposure and may reflect both direct effects on the pup and indirect effect from drug-induced alterations in maternal metabolism or behavior.", "PMID": 978475}
{"id": "pubmed23n0032_11740", "title": "The deveolopment of a radioimmunoassy for detection of cocaine metabolites.", "content": "This paper describes the production of antibenzoylecgonine (BE) serum in rabbits and sheep from an ecgonine-sheep gamma globulin conjugate. With the use of this antiserum a radioimmunoassay was developed capable of detecting 5 to 10 ng of benzoylecgonine, a major cocaine metabolite in human urine. The antibody recognized ecgonine approximately 1/2, benzoylnorecgonine 1/10, cocaine, 1/5, and norcocaine and ecgonine methyl ester less than 1/50 as much as benzoylecgonine. The radioimmunoassay has been utilized in the measurement of benzoylecgonine levels in human urines and in organs and biological fluids of rabbits and rats. Qualitative results were compared with the Enzyme Multiplied Immunoassay Technique and thin-layer chromatography and found to be in good agreement. The technique is the most sensitive one available for monitoring cocaine ingestion by man and allows for specific determination of benzoylecgonine directly in urine without extraction and/or derivatization. Although ecgonine-sheep gamma globulin was used as the immunogen, the total antibody produced by both species was more reactive with respect to BE. The greater reactivity to BE suggests a possible conversion of a significant portion of the conjugated ecgonine in vivo to compound(s) conformationally more closely related to BE against which the major portion of the total antibody subsequently elicited was directed.", "contents": "The deveolopment of a radioimmunoassy for detection of cocaine metabolites. This paper describes the production of antibenzoylecgonine (BE) serum in rabbits and sheep from an ecgonine-sheep gamma globulin conjugate. With the use of this antiserum a radioimmunoassay was developed capable of detecting 5 to 10 ng of benzoylecgonine, a major cocaine metabolite in human urine. The antibody recognized ecgonine approximately 1/2, benzoylnorecgonine 1/10, cocaine, 1/5, and norcocaine and ecgonine methyl ester less than 1/50 as much as benzoylecgonine. The radioimmunoassay has been utilized in the measurement of benzoylecgonine levels in human urines and in organs and biological fluids of rabbits and rats. Qualitative results were compared with the Enzyme Multiplied Immunoassay Technique and thin-layer chromatography and found to be in good agreement. The technique is the most sensitive one available for monitoring cocaine ingestion by man and allows for specific determination of benzoylecgonine directly in urine without extraction and/or derivatization. Although ecgonine-sheep gamma globulin was used as the immunogen, the total antibody produced by both species was more reactive with respect to BE. The greater reactivity to BE suggests a possible conversion of a significant portion of the conjugated ecgonine in vivo to compound(s) conformationally more closely related to BE against which the major portion of the total antibody subsequently elicited was directed.", "PMID": 978476}
{"id": "pubmed23n0032_11741", "title": "Mode of action of trichloroethylene on squid axon membranes.", "content": "The mode of action of trichloroethylene on electrical properties of squid giant axons has been studied by means of voltage clamp techniques. Trichloroethylene decreased the resting membrane potential in a manner dependent upon the concentration, the depolarization by 50% saturated trichloroethylene attaining 28.4 and 32.7% of the initial value at 20 and 10 degrees C, respectively. Leakage conductance was decreased to 34.6% of the control by 30% saturated trichloroethylene at 10-12 degrees C. It appears that the trichloroethylene-induced depolarization is at least in part due to a decrease in resting potassium permeability. Both peak transient and steady-state conductance increases were suppressec by trichloroethylene, and the curve relating the steady-state conductance to the membrane potential was shifted in the depolarizing direction while the peak transient conductance curve was not appreciably shifted. The reversal potential for the peak transient current was greatly shifted by trichloroethylene in the direction of hyperpolarization in a manner dependent on the concentration, the maximum shift amounting to 25 mV at 10 degrees C. This effect was less pronounced at 20 degrees C. The shift in the reversal potential is mostly due to a decrease in selectivity of the peak transient channel and partly due to an accumulation of sodium ions inside. Analyes of dose-response relation in suppressing peak transient and steady-state conductances show that trichloroethylene interacts with receptor on a one-to-one stoichiometric basis. Steady-state sodium inactivation curve was shifted by trichloroethylene in the direction of hyperpolarization. All of these effects were partially reversed after washing the axon with anesthetic-free media. The accumulation of sodium ions inside would be much more pronounced in small nerve fibers in the brain than in giant axon and, together with the observed decrease in the selectivity of peak transient channels, would play a significant role in general anesthesia.", "contents": "Mode of action of trichloroethylene on squid axon membranes. The mode of action of trichloroethylene on electrical properties of squid giant axons has been studied by means of voltage clamp techniques. Trichloroethylene decreased the resting membrane potential in a manner dependent upon the concentration, the depolarization by 50% saturated trichloroethylene attaining 28.4 and 32.7% of the initial value at 20 and 10 degrees C, respectively. Leakage conductance was decreased to 34.6% of the control by 30% saturated trichloroethylene at 10-12 degrees C. It appears that the trichloroethylene-induced depolarization is at least in part due to a decrease in resting potassium permeability. Both peak transient and steady-state conductance increases were suppressec by trichloroethylene, and the curve relating the steady-state conductance to the membrane potential was shifted in the depolarizing direction while the peak transient conductance curve was not appreciably shifted. The reversal potential for the peak transient current was greatly shifted by trichloroethylene in the direction of hyperpolarization in a manner dependent on the concentration, the maximum shift amounting to 25 mV at 10 degrees C. This effect was less pronounced at 20 degrees C. The shift in the reversal potential is mostly due to a decrease in selectivity of the peak transient channel and partly due to an accumulation of sodium ions inside. Analyes of dose-response relation in suppressing peak transient and steady-state conductances show that trichloroethylene interacts with receptor on a one-to-one stoichiometric basis. Steady-state sodium inactivation curve was shifted by trichloroethylene in the direction of hyperpolarization. All of these effects were partially reversed after washing the axon with anesthetic-free media. The accumulation of sodium ions inside would be much more pronounced in small nerve fibers in the brain than in giant axon and, together with the observed decrease in the selectivity of peak transient channels, would play a significant role in general anesthesia.", "PMID": 978477}
{"id": "pubmed23n0032_11742", "title": "Uptake and metabolism of nicotine by the isolated perfused rabbit lung.", "content": "In order to investigate the possibility of pulmonary first-pass metabolism of nicotine inhaled in tobacco smoke, the absorption and disposition of 14C-nicotine were studied in an isolated perfused rabbit lung preparation after nicotine administration directly into the perfusing blood and tobacco smoke administration via in the inspired tracheal air. After administration into the perfusing medium, the rate of nicotine metabolism was first-order and dose-independent at the two doses studies (0.1 and 1.0 mg) but lung metabolic clearance was quite low (3 ml/min) relative to whole body clearance (140 ml/min) measured by administering 14C-nicotine to intact rabbits. Accumulation of nicotine by lung was not extensive (13-23% of the dose administered). After administration of tobacco smoke from 14C-nicotine-spiked cigarettes, absorption of nicotine was rapid but the rate of metabolism was markedly reduced compared to the studies in which drug was administered in the perfusing medium. This reduction in the rate of metabolism was apparently caused by some component of tobacco smoke but was shown to be unrelated to the level of carbon monoxide in the perfusate. The slow clearance of nicotine by rabbit lung (which is further reduced after smoke administration) compared to a high pulmonary blood flow rate makes unlikely the possibility of significant first-pass lung metabolism in smokers.", "contents": "Uptake and metabolism of nicotine by the isolated perfused rabbit lung. In order to investigate the possibility of pulmonary first-pass metabolism of nicotine inhaled in tobacco smoke, the absorption and disposition of 14C-nicotine were studied in an isolated perfused rabbit lung preparation after nicotine administration directly into the perfusing blood and tobacco smoke administration via in the inspired tracheal air. After administration into the perfusing medium, the rate of nicotine metabolism was first-order and dose-independent at the two doses studies (0.1 and 1.0 mg) but lung metabolic clearance was quite low (3 ml/min) relative to whole body clearance (140 ml/min) measured by administering 14C-nicotine to intact rabbits. Accumulation of nicotine by lung was not extensive (13-23% of the dose administered). After administration of tobacco smoke from 14C-nicotine-spiked cigarettes, absorption of nicotine was rapid but the rate of metabolism was markedly reduced compared to the studies in which drug was administered in the perfusing medium. This reduction in the rate of metabolism was apparently caused by some component of tobacco smoke but was shown to be unrelated to the level of carbon monoxide in the perfusate. The slow clearance of nicotine by rabbit lung (which is further reduced after smoke administration) compared to a high pulmonary blood flow rate makes unlikely the possibility of significant first-pass lung metabolism in smokers.", "PMID": 978478}
{"id": "pubmed23n0032_11743", "title": "The effects of physostigmine on cholinesterase activity, storage and release of acetylcholine in the isolated chicken heart.", "content": "Total activity of the cholinesterases (ChE) and the concentration and output of acetylcholine (ACh) were measured in isolated chicken hearts. The ACh concentration of the right ventricle was as high as that of the total heart (approximately 7 nmol/g). The ChE activity and its kinetic parameters did not differ from those of other vertebrates. In the absence of physostigmine, stimulation of both vagus nerves (20 Hz, 5 minutes) caused an output of ACh and a decrease in rate (-90% of control) and amplitude (-74%) of the ventricular contraction. Physostigmine (10(-7) M) inhibited the ChE by 87%, caused a small reduction of the ACh concentration (P = .05) and more than doubled the ACh output in response to vagal stimulation. In contrast, 10(-6) M physostigmine increased significantly the ACh concentration by about 40% within 5 minutes; the output was almost 3-fold higher than that in the absence of physostigmine and the ChE was nearly completely inhibited (-96%); the output of ACh in the 5th minute of stimulation was not significantly lower than that in the 1st minute. Physostigmine, in a concentration of 10(-4) M, did not change the ACh content of the heart and was much less effective in elevating the ACh output than was a concentration of physostigmine of 10(-6) M. In conclusion, the high ACh output from isolated chicken hearts evoked by preganglionic nerve stimulation was at least partially due to the pronounced innervation of the avian ventricles. Inhibition of the ChE by 96% failed to cause ganglionic transmission block. The formation of surplus ACh required higher physostigmine concentrations (10(-6) M) than the inhibition of the external ChE (protection of ACh released). High physostigmine concentrations (greater than 10(-6) M) counteracted the formation of surplus ACh.", "contents": "The effects of physostigmine on cholinesterase activity, storage and release of acetylcholine in the isolated chicken heart. Total activity of the cholinesterases (ChE) and the concentration and output of acetylcholine (ACh) were measured in isolated chicken hearts. The ACh concentration of the right ventricle was as high as that of the total heart (approximately 7 nmol/g). The ChE activity and its kinetic parameters did not differ from those of other vertebrates. In the absence of physostigmine, stimulation of both vagus nerves (20 Hz, 5 minutes) caused an output of ACh and a decrease in rate (-90% of control) and amplitude (-74%) of the ventricular contraction. Physostigmine (10(-7) M) inhibited the ChE by 87%, caused a small reduction of the ACh concentration (P = .05) and more than doubled the ACh output in response to vagal stimulation. In contrast, 10(-6) M physostigmine increased significantly the ACh concentration by about 40% within 5 minutes; the output was almost 3-fold higher than that in the absence of physostigmine and the ChE was nearly completely inhibited (-96%); the output of ACh in the 5th minute of stimulation was not significantly lower than that in the 1st minute. Physostigmine, in a concentration of 10(-4) M, did not change the ACh content of the heart and was much less effective in elevating the ACh output than was a concentration of physostigmine of 10(-6) M. In conclusion, the high ACh output from isolated chicken hearts evoked by preganglionic nerve stimulation was at least partially due to the pronounced innervation of the avian ventricles. Inhibition of the ChE by 96% failed to cause ganglionic transmission block. The formation of surplus ACh required higher physostigmine concentrations (10(-6) M) than the inhibition of the external ChE (protection of ACh released). High physostigmine concentrations (greater than 10(-6) M) counteracted the formation of surplus ACh.", "PMID": 978479}
{"id": "pubmed23n0032_11744", "title": "Maturation of renal organic acid transport in vivo: substrate stimulation by penicillin.", "content": "These experiments were designed to demonstrate that pretreatment of newborn dogs with penicillin will enhance maturation of the renal organic anion transport system in vivo. Littermate pups were injected with procaine penicillin G (300,000 I.U./kg b.i.d. for 3 days) or saline before 2 weeks of age. Animals were surgically prepared on day 16 or 17. Transport of p-aminhippurate (PAH), as an indicator of renal organic anion transport, was quantified by the following procedures: 1) disappearance of a bolus injection from the plasma, 2) renal extraction and 3) rate of secretion in steady-state clearance experiments. The renal handling of insulin was measured simultaneously in all procedures for comparison. Penicillin-treated animals excreted a single intravenous bolus of PAH more rapidly than control animals, with the T1/2 decreased and the constant of elimination (Ke) increased. The extraction of PAH by kidneys of penicillin-treated animals was 165% that of controls. Similarly, the tubular secretion of PAH was increased in treated pups. These data confirm that in newborn animals the renal handling of organic anions in vivo is enhanced by pretreatment with a substrate of the secretory system.", "contents": "Maturation of renal organic acid transport in vivo: substrate stimulation by penicillin. These experiments were designed to demonstrate that pretreatment of newborn dogs with penicillin will enhance maturation of the renal organic anion transport system in vivo. Littermate pups were injected with procaine penicillin G (300,000 I.U./kg b.i.d. for 3 days) or saline before 2 weeks of age. Animals were surgically prepared on day 16 or 17. Transport of p-aminhippurate (PAH), as an indicator of renal organic anion transport, was quantified by the following procedures: 1) disappearance of a bolus injection from the plasma, 2) renal extraction and 3) rate of secretion in steady-state clearance experiments. The renal handling of insulin was measured simultaneously in all procedures for comparison. Penicillin-treated animals excreted a single intravenous bolus of PAH more rapidly than control animals, with the T1/2 decreased and the constant of elimination (Ke) increased. The extraction of PAH by kidneys of penicillin-treated animals was 165% that of controls. Similarly, the tubular secretion of PAH was increased in treated pups. These data confirm that in newborn animals the renal handling of organic anions in vivo is enhanced by pretreatment with a substrate of the secretory system.", "PMID": 978480}
{"id": "pubmed23n0032_11745", "title": "Effect of dihydro-ouabain on vascular tone on the perfused cannine hindlimb.", "content": "Digitalis compounds are known to cause contraction of vascular smooth muscle. However, it has been reported by Kahn and colleagues (J. Pharmacol. Exp. Ther. 142: 215-222, 1963) that dihydro-ouabain has no effect on vascular tone in dogs. The purpose of our study was to reassess the vasoconstrictor activity of dihydro-ouabain. This was done by monitoring perfusion pressure of the canine hindlimb preparation perfused at a constant rate of blood flow and administering intra-arterial injections of dihydro-ouabain. Increasing doses of this drug (i.e., 25, 50, 100, 200 and 300 mug) produced significant dose-dependent increases in perfusion pressure. Pretreatment with phentolamine prevented the effect of dihydro-ouabain on perfusion pressure. These results indicate that dihydro-ouabain causes contraction of vascular smooth muscle by an adrenergic mechanism.", "contents": "Effect of dihydro-ouabain on vascular tone on the perfused cannine hindlimb. Digitalis compounds are known to cause contraction of vascular smooth muscle. However, it has been reported by Kahn and colleagues (J. Pharmacol. Exp. Ther. 142: 215-222, 1963) that dihydro-ouabain has no effect on vascular tone in dogs. The purpose of our study was to reassess the vasoconstrictor activity of dihydro-ouabain. This was done by monitoring perfusion pressure of the canine hindlimb preparation perfused at a constant rate of blood flow and administering intra-arterial injections of dihydro-ouabain. Increasing doses of this drug (i.e., 25, 50, 100, 200 and 300 mug) produced significant dose-dependent increases in perfusion pressure. Pretreatment with phentolamine prevented the effect of dihydro-ouabain on perfusion pressure. These results indicate that dihydro-ouabain causes contraction of vascular smooth muscle by an adrenergic mechanism.", "PMID": 978481}
{"id": "pubmed23n0032_11746", "title": "Effect of nitroprusside on smooth muscle and adrenergic nerve terminals in isolated blood vessels.", "content": "Experiments were designed to assess the mode of action of nitroprusside on isolated blood vessels and its relative potency on venous and arterial smooth muscle. Strips from dog blood vessels were mounted in an organ bath for isometric tension recording. Sodium nitroprusside (10(-5) M) depressed the contraction of saphenous vein strips caused by electric stimulation, tyramine, K+, Ba++, norepinephrine and acetylcholine. The depression of the norepinephrine-induced contractions also occurred in a Ca++- free medium and when Ca++ influx was inhibited by verapamil. Nitroprusside reduced the frequency of the spontaneous contractions of strips of portal-mesenteric veins. It depressed the contraction caused by norepinephrine in tibial artery strips more than in saphenous vein strips. Saphenous vein strips were incubated with (3H)norepinephrine and mounted for superfusion and isometric tension recording. Sodium nitroprusside (10(-5) M) had no effect on the basal efflux of 3H compounds. During electric stimulation, it did not change the output of (3H)norepinephrine but increased the outflow of deaminated and O-methylated metabolites. Thus sodium nitroprusside 1) has a direct effect on the smooth muscle cells which is independent of Ca++ influx, 2) depresses contractions of different types of vascular smooth muscle and 3) does not inhibit the release of norepinephrine from the nerve endings.", "contents": "Effect of nitroprusside on smooth muscle and adrenergic nerve terminals in isolated blood vessels. Experiments were designed to assess the mode of action of nitroprusside on isolated blood vessels and its relative potency on venous and arterial smooth muscle. Strips from dog blood vessels were mounted in an organ bath for isometric tension recording. Sodium nitroprusside (10(-5) M) depressed the contraction of saphenous vein strips caused by electric stimulation, tyramine, K+, Ba++, norepinephrine and acetylcholine. The depression of the norepinephrine-induced contractions also occurred in a Ca++- free medium and when Ca++ influx was inhibited by verapamil. Nitroprusside reduced the frequency of the spontaneous contractions of strips of portal-mesenteric veins. It depressed the contraction caused by norepinephrine in tibial artery strips more than in saphenous vein strips. Saphenous vein strips were incubated with (3H)norepinephrine and mounted for superfusion and isometric tension recording. Sodium nitroprusside (10(-5) M) had no effect on the basal efflux of 3H compounds. During electric stimulation, it did not change the output of (3H)norepinephrine but increased the outflow of deaminated and O-methylated metabolites. Thus sodium nitroprusside 1) has a direct effect on the smooth muscle cells which is independent of Ca++ influx, 2) depresses contractions of different types of vascular smooth muscle and 3) does not inhibit the release of norepinephrine from the nerve endings.", "PMID": 978482}
{"id": "pubmed23n0032_11747", "title": "Metabolic activation of furosemide to a chemically reactive, hepatotoxic metabolite.", "content": "The possibility that furosemide-induced hepatic necrosis results from the formation of a chemically reactive hepatotoxic metabolite has been examined. Hepatotoxic doses of 3H-furosemide or 14C-furosemide were administered to normal mice and to mice pretreated with piperonyl butoxide, cobalt chloride, alpha-naphthylisothiocyanate or phenobarbital. Mice were killed at various time intervals and tissues were examined for necrosis, for free furosemide concentrations and for covalently bound metabolites of furosemide. Little furosemide was covalently bound to muscle, whereas the amount of covalently bound material in liver usually paralleled the severity of live necrosis after alteration by the pretreatments. The severity of hepatic necrosis failed to correlate with furosemide concentrations in liver or plasma. Furosemide was shown to be metabolically activated to an arylating intermediate by a cytochrome P-450 mixed function oxidase in hepatic microsomes. Additional experiments demonstrated that the furan ring of furosemide was the portion activated.", "contents": "Metabolic activation of furosemide to a chemically reactive, hepatotoxic metabolite. The possibility that furosemide-induced hepatic necrosis results from the formation of a chemically reactive hepatotoxic metabolite has been examined. Hepatotoxic doses of 3H-furosemide or 14C-furosemide were administered to normal mice and to mice pretreated with piperonyl butoxide, cobalt chloride, alpha-naphthylisothiocyanate or phenobarbital. Mice were killed at various time intervals and tissues were examined for necrosis, for free furosemide concentrations and for covalently bound metabolites of furosemide. Little furosemide was covalently bound to muscle, whereas the amount of covalently bound material in liver usually paralleled the severity of live necrosis after alteration by the pretreatments. The severity of hepatic necrosis failed to correlate with furosemide concentrations in liver or plasma. Furosemide was shown to be metabolically activated to an arylating intermediate by a cytochrome P-450 mixed function oxidase in hepatic microsomes. Additional experiments demonstrated that the furan ring of furosemide was the portion activated.", "PMID": 978483}
{"id": "pubmed23n0032_11748", "title": "Comparison of the effect of carbon monoxide and of hypoxic hypoxia. I. In vivo metabolism, distribution and action of hexobarbital.", "content": "Previous experiments had shown that carbon monoxide inhalation prolonged the in vivo response zoxazolamine. However, CO was clearly less potent than an equivalent level of hypoxia induced by inhalation of lowered O2 content (hypoxic hypoxia). The present study revealed that both CO and hypoxic hypoxia also prolonged the pharmacologic response to a drug of vastly different structure and site of action, i.e., hexobarbital. In this case, CO was approximately equipotent to hypoxic hypoxia in prolonging sleeping time. In spite of a number of significant effects of the two types of hypoxia on factors such as rate of peritoneal absorption, distribution into the brain and brain sensitivity to the drug, their relative potencies in prolonging sleeping time appeared to reflect adequately their relative effects on in vivo rate of metabolism. This conclusion was based on their equipotencies in decreasing the rate of disappearance of hexobarbital from the blood. The observation that hypoxia induced by CO had no greater effect on hexobarbital metabolism than hypoxia induced by oxygen deprivation suggests that the binding of CO to cytochrome P-450 is not important in drug metabolism in vivo. CO would appear to act solely by inducing tissue hypoxia.", "contents": "Comparison of the effect of carbon monoxide and of hypoxic hypoxia. I. In vivo metabolism, distribution and action of hexobarbital. Previous experiments had shown that carbon monoxide inhalation prolonged the in vivo response zoxazolamine. However, CO was clearly less potent than an equivalent level of hypoxia induced by inhalation of lowered O2 content (hypoxic hypoxia). The present study revealed that both CO and hypoxic hypoxia also prolonged the pharmacologic response to a drug of vastly different structure and site of action, i.e., hexobarbital. In this case, CO was approximately equipotent to hypoxic hypoxia in prolonging sleeping time. In spite of a number of significant effects of the two types of hypoxia on factors such as rate of peritoneal absorption, distribution into the brain and brain sensitivity to the drug, their relative potencies in prolonging sleeping time appeared to reflect adequately their relative effects on in vivo rate of metabolism. This conclusion was based on their equipotencies in decreasing the rate of disappearance of hexobarbital from the blood. The observation that hypoxia induced by CO had no greater effect on hexobarbital metabolism than hypoxia induced by oxygen deprivation suggests that the binding of CO to cytochrome P-450 is not important in drug metabolism in vivo. CO would appear to act solely by inducing tissue hypoxia.", "PMID": 978484}
{"id": "pubmed23n0032_11749", "title": "Comparison of the effect of carbon monoxide and of hypoxic hypoxia. II. Hexobarbital metabolism in the isolated, perfused rat liver.", "content": "Rat livers were perfused with a red blood cell-containing perfusion medium. The rate of metabolism of hexobarbital was determined in this system as the recirculating perfusion fluid was made hypoxic by exposure to either CO or lowered oxygen tension. It was found that 7.6% of the total oxygen consumed by the liver was utilized to metabolize hexobarbital regardless of the severity of the hypoxia, which suggests that the liver does not shunt O2 to more preferential pathways as O2 availability becomes limited. When oxygen delivery was lowered below 0.3 ml of O2 per min per g of liver by either type of hypoxia, the rate of hexobarbital metabolism decreased. However, based on the rate of oxygen delivery, CO was more potent than hypoxic hypoxia in inhibiting drug metabolism. On the other hand, the two types of hypoxia were equipotent when compared on the basis of alterations in oxygen consumption. These results are consistent with the known effect of CO in decreasing the availability of hemoglobin-bound O2 and further argue against the direct inhibition of drug metabolism by the binding of CO to cytochrome P-450 in the isolated liver. They also suggest that the lack of a greater potency of CO relative to hypoxic hypoxia in vivo might be related to alterations in hepatic blood flow.", "contents": "Comparison of the effect of carbon monoxide and of hypoxic hypoxia. II. Hexobarbital metabolism in the isolated, perfused rat liver. Rat livers were perfused with a red blood cell-containing perfusion medium. The rate of metabolism of hexobarbital was determined in this system as the recirculating perfusion fluid was made hypoxic by exposure to either CO or lowered oxygen tension. It was found that 7.6% of the total oxygen consumed by the liver was utilized to metabolize hexobarbital regardless of the severity of the hypoxia, which suggests that the liver does not shunt O2 to more preferential pathways as O2 availability becomes limited. When oxygen delivery was lowered below 0.3 ml of O2 per min per g of liver by either type of hypoxia, the rate of hexobarbital metabolism decreased. However, based on the rate of oxygen delivery, CO was more potent than hypoxic hypoxia in inhibiting drug metabolism. On the other hand, the two types of hypoxia were equipotent when compared on the basis of alterations in oxygen consumption. These results are consistent with the known effect of CO in decreasing the availability of hemoglobin-bound O2 and further argue against the direct inhibition of drug metabolism by the binding of CO to cytochrome P-450 in the isolated liver. They also suggest that the lack of a greater potency of CO relative to hypoxic hypoxia in vivo might be related to alterations in hepatic blood flow.", "PMID": 978485}
{"id": "pubmed23n0032_11750", "title": "Studies of the metabolism of 5,5-diphenylhydantoin relating principally to the stereoselectivity of the hydroxylation reactions in man and the dog.", "content": "The hydroxylated metabolites of 5,5-diphenylhydantoin (DPH) in dog and human urine, after release by beta-glucuronidase, have been isolated and purified by procedures entailing only solvent partitioning without crystallization or any other procedure that could change the proportions of optical isomers. Dogs produced both the meta- and para-5-hydroxyphenyl-5-phenylhydantoins (m-HPPH and p-HPPH) from DPH, the former in approximately 6 times the amount of the latter. The m-HPPH from dog urine was dextrorotatory and had the properties of a pure optical isomer. The p-HPPH from dog urine was a mixture of optical isomers with approximately a 2:1 preponderance of the levorotatory isomer. When dog urine was heated with acid rather than treated with beta-glucuronidase, the isolated p-HPPH contained a small preponderance of the dextrorotatory isomer, probably owing to production of d-p-HPPH from the dihydrodiol metabolite. No o-HPPH was found in dog urine. When racemic p-HPPH and m-HPPH were administered to dogs, there was little if any preponderance of either optical isomer in the materials isolated from urine. Enzymatically released p-HPPH isolated from urine of human patients receiving DPH was a mixture of optical isomers with approximarely a 10:1 preponderance of the levorotatory isomer. Crystallization of this material readily yields pure l-p-HPPH. Human urine contained only very small amounts of m-HPPH, which was detectable by gas chromatography but insufficient in amount to permit isolation. Hypotheses are discussed that could account for the differences in the metabolism of DPH in man and dog. Small amounts of 2,2-diphenylhydantoic acid were found in urine of dogs but not of human patients receiving DPH.", "contents": "Studies of the metabolism of 5,5-diphenylhydantoin relating principally to the stereoselectivity of the hydroxylation reactions in man and the dog. The hydroxylated metabolites of 5,5-diphenylhydantoin (DPH) in dog and human urine, after release by beta-glucuronidase, have been isolated and purified by procedures entailing only solvent partitioning without crystallization or any other procedure that could change the proportions of optical isomers. Dogs produced both the meta- and para-5-hydroxyphenyl-5-phenylhydantoins (m-HPPH and p-HPPH) from DPH, the former in approximately 6 times the amount of the latter. The m-HPPH from dog urine was dextrorotatory and had the properties of a pure optical isomer. The p-HPPH from dog urine was a mixture of optical isomers with approximately a 2:1 preponderance of the levorotatory isomer. When dog urine was heated with acid rather than treated with beta-glucuronidase, the isolated p-HPPH contained a small preponderance of the dextrorotatory isomer, probably owing to production of d-p-HPPH from the dihydrodiol metabolite. No o-HPPH was found in dog urine. When racemic p-HPPH and m-HPPH were administered to dogs, there was little if any preponderance of either optical isomer in the materials isolated from urine. Enzymatically released p-HPPH isolated from urine of human patients receiving DPH was a mixture of optical isomers with approximarely a 10:1 preponderance of the levorotatory isomer. Crystallization of this material readily yields pure l-p-HPPH. Human urine contained only very small amounts of m-HPPH, which was detectable by gas chromatography but insufficient in amount to permit isolation. Hypotheses are discussed that could account for the differences in the metabolism of DPH in man and dog. Small amounts of 2,2-diphenylhydantoic acid were found in urine of dogs but not of human patients receiving DPH.", "PMID": 978486}
{"id": "pubmed23n0032_11751", "title": "Role of metabolism in the biliary excretion of methadone metabolites.", "content": "The biliary excretion of the major methadone metabolites was studied after the administration of purified 14C-labeled metabolites of methadone. The three methadone metabolites administered to rats were the pyrrolidine and pyrroline metabolites and the glucuronide conjugate of the hydroxylated pyrroline metabolite. We found that the excretion of 14C into bile was greater after administration of the metabolites which underwent fewer metabolic steps prior to biliary excretion. Phenobarbital pretreatment of rats increased the biliary excretion of 14C after the administration of the 14C-labeled pyrrolidine and pyrroline metabolites, both of which undergo further metabolism. Phenobarbital pretreatment did not change the biliary excretion of 14C-labeled glucuronide of the pyrroline metabolite which does not undergo further metabolism in the liver. Analysis of bile samples showed that the increased biliary excretion of 14C in phenobarbital-pretreated rats after administration of the labeled pyrrolidine and pyrroline metabolites was due primarily to increases in the biliary excretion of methadone metabolites which resulted from further in vivo metabolism of the injected metabolites. These data confirm our earlier suggestion (Roerig et al., Biochem. Pharmacol. 24: 355-362, 1975) that phenobarbital pretreatment increases biliary excretion of methadone metabolites by increasing hepatic metabolism of methadone. Furthermore, it can be concluded from these studies that metabolism is a rate-limiting process in the excretion of methadone metabolites in bile.", "contents": "Role of metabolism in the biliary excretion of methadone metabolites. The biliary excretion of the major methadone metabolites was studied after the administration of purified 14C-labeled metabolites of methadone. The three methadone metabolites administered to rats were the pyrrolidine and pyrroline metabolites and the glucuronide conjugate of the hydroxylated pyrroline metabolite. We found that the excretion of 14C into bile was greater after administration of the metabolites which underwent fewer metabolic steps prior to biliary excretion. Phenobarbital pretreatment of rats increased the biliary excretion of 14C after the administration of the 14C-labeled pyrrolidine and pyrroline metabolites, both of which undergo further metabolism. Phenobarbital pretreatment did not change the biliary excretion of 14C-labeled glucuronide of the pyrroline metabolite which does not undergo further metabolism in the liver. Analysis of bile samples showed that the increased biliary excretion of 14C in phenobarbital-pretreated rats after administration of the labeled pyrrolidine and pyrroline metabolites was due primarily to increases in the biliary excretion of methadone metabolites which resulted from further in vivo metabolism of the injected metabolites. These data confirm our earlier suggestion (Roerig et al., Biochem. Pharmacol. 24: 355-362, 1975) that phenobarbital pretreatment increases biliary excretion of methadone metabolites by increasing hepatic metabolism of methadone. Furthermore, it can be concluded from these studies that metabolism is a rate-limiting process in the excretion of methadone metabolites in bile.", "PMID": 978487}
{"id": "pubmed23n0032_11752", "title": "Ischemic changes in the canine heart as affected by the dimethyl quaternary analog of propranolol, UM-272 (SC-27761).", "content": "The effects of the dimethyl quarternary analog of propranolol, UM-272, on myocardial infarct volume were studied in the canine heart. Myocardial infarction was produced by occlusion of the left circumflex coronary artery for 60 minutes followed by reperfusion and quantitation of infarct volume 24 hours later. Groups of dogs were either untreated or pretreated with UM-272 with an initial loading dose of 5.0 mg/kg (group A) or 2.5 mg/kg (group B) 30 minutes before occlusion of the left circumflex coronary artery. Both group A and group B animals received additional doses of 2.5 mg/kg of UM-272 every 90 minutes for a period of 6 hours so that the total respective doses were 15 and 12.5 mg/kg. Control animals received comparable volumes of 0.9% sodium chloride solution. All animals were followed throughout the 6-hour procedure with continuous electrocardiographic recordings which were used to assess the effects of acute myocardial ischemia upon disturbances in cardiac rhythm and the effects of drug treatment. Dogs which survived the procedure were given tetracycline i.v. the next day and sacrificed 1 hour later by an overdose of pentobarbital sodium. The hearts were removed and the left ventricle was sliced and examined first under ultraviolet light to localize the ischemic zone by noting the tetracycline fluorescence. The ventricular slices were next incubated in nitro blue tetrazolium which stains normal myocardial tissue, thus allowing one to quantitate the volume of infarcted myocardium by excising and weighing the nonstained and stained muscle separately. The untreated control group had an infarct volume of 23.8 +/- 3.2 g/100 g of left ventricle. The treated animals in groups A and B had respective infarct volumes of 2.3 +/- 0.8 g/100 g (P less than .001) and 7.0 +/- 3.3 g/100 g (P less than .025) of left ventricle. During the acute phase of ischemia and reperfusion, arrhythmias and alterations in the ST-segment, R-wave amplituted and development of pathologic Q-waves were more prominent in the untreated animals and almost totally absent in the treated animals. UM-272 produced a dose-dependent decrease in heart rate as well as a decrease in developed isometric tension. Pretreatment with UM-272 did not prevent the derangement of function in the ischemic zone nor did it permit a return of function upon reperfusion, even though it reduced the degree of cellular damage resulting from 60 minutes of regional ischemia. A possible mechanism for the protective effect of UM-272 may be through its ability to reduce myocardial contractility and heart rate, both of which would reduce myocardial oxygen consumption and thus produce a more favorable balance between myocardial oxygen supply and myocardial oxygen demand.", "contents": "Ischemic changes in the canine heart as affected by the dimethyl quaternary analog of propranolol, UM-272 (SC-27761). The effects of the dimethyl quarternary analog of propranolol, UM-272, on myocardial infarct volume were studied in the canine heart. Myocardial infarction was produced by occlusion of the left circumflex coronary artery for 60 minutes followed by reperfusion and quantitation of infarct volume 24 hours later. Groups of dogs were either untreated or pretreated with UM-272 with an initial loading dose of 5.0 mg/kg (group A) or 2.5 mg/kg (group B) 30 minutes before occlusion of the left circumflex coronary artery. Both group A and group B animals received additional doses of 2.5 mg/kg of UM-272 every 90 minutes for a period of 6 hours so that the total respective doses were 15 and 12.5 mg/kg. Control animals received comparable volumes of 0.9% sodium chloride solution. All animals were followed throughout the 6-hour procedure with continuous electrocardiographic recordings which were used to assess the effects of acute myocardial ischemia upon disturbances in cardiac rhythm and the effects of drug treatment. Dogs which survived the procedure were given tetracycline i.v. the next day and sacrificed 1 hour later by an overdose of pentobarbital sodium. The hearts were removed and the left ventricle was sliced and examined first under ultraviolet light to localize the ischemic zone by noting the tetracycline fluorescence. The ventricular slices were next incubated in nitro blue tetrazolium which stains normal myocardial tissue, thus allowing one to quantitate the volume of infarcted myocardium by excising and weighing the nonstained and stained muscle separately. The untreated control group had an infarct volume of 23.8 +/- 3.2 g/100 g of left ventricle. The treated animals in groups A and B had respective infarct volumes of 2.3 +/- 0.8 g/100 g (P less than .001) and 7.0 +/- 3.3 g/100 g (P less than .025) of left ventricle. During the acute phase of ischemia and reperfusion, arrhythmias and alterations in the ST-segment, R-wave amplituted and development of pathologic Q-waves were more prominent in the untreated animals and almost totally absent in the treated animals. UM-272 produced a dose-dependent decrease in heart rate as well as a decrease in developed isometric tension. Pretreatment with UM-272 did not prevent the derangement of function in the ischemic zone nor did it permit a return of function upon reperfusion, even though it reduced the degree of cellular damage resulting from 60 minutes of regional ischemia. A possible mechanism for the protective effect of UM-272 may be through its ability to reduce myocardial contractility and heart rate, both of which would reduce myocardial oxygen consumption and thus produce a more favorable balance between myocardial oxygen supply and myocardial oxygen demand.", "PMID": 978488}
{"id": "pubmed23n0032_11753", "title": "Phentolamine and isoproterenol: comparison of effects on vascular resistance and oxygen uptake in skeletal muscle during hypotension.", "content": "Effects of drugs on precapillary vessels and oxygen uptake may differ from effects on resistance vessels and total flow in skeletal muscle. This study was performed to compare effects of phentolamine and isoproterenol, two drugs which are used to treat shock, on oxygen uptake in skeletal muscle. Oxygen uptake of gracilis muscle (GVO2) was measured in muscles perfused at constant flow or constant pressure in normotensive dogs. We compared effects of the drugs on oxygen uptake at doses chosen so that both drugs produced comparable effects on vascular resistance. With flow constant, phentolamine increased but isoproterenol decreased or did not alter GVO2. With constant pressure, phentolamine produced significantly greater increases in GVO2. For example, increases in GVO2 occurred with all three doses of phentolamine, but only with the high dose of isoproterenol. Neither drug altered oxygen-hemoglobin affinity of red blood cells or oxygen consumption of skeletal muscle in vitro. The results suggest that phentolamine produces more favorable effects than isoproterenol on oxygen uptake in skeletal muscle, presumably because of greater dilator action on precapillary sphincters.", "contents": "Phentolamine and isoproterenol: comparison of effects on vascular resistance and oxygen uptake in skeletal muscle during hypotension. Effects of drugs on precapillary vessels and oxygen uptake may differ from effects on resistance vessels and total flow in skeletal muscle. This study was performed to compare effects of phentolamine and isoproterenol, two drugs which are used to treat shock, on oxygen uptake in skeletal muscle. Oxygen uptake of gracilis muscle (GVO2) was measured in muscles perfused at constant flow or constant pressure in normotensive dogs. We compared effects of the drugs on oxygen uptake at doses chosen so that both drugs produced comparable effects on vascular resistance. With flow constant, phentolamine increased but isoproterenol decreased or did not alter GVO2. With constant pressure, phentolamine produced significantly greater increases in GVO2. For example, increases in GVO2 occurred with all three doses of phentolamine, but only with the high dose of isoproterenol. Neither drug altered oxygen-hemoglobin affinity of red blood cells or oxygen consumption of skeletal muscle in vitro. The results suggest that phentolamine produces more favorable effects than isoproterenol on oxygen uptake in skeletal muscle, presumably because of greater dilator action on precapillary sphincters.", "PMID": 978489}
{"id": "pubmed23n0032_11754", "title": "Uptake, metabolism and efflux of methadone in \"single pass\" isolated perfused rabbit lungs.", "content": "The uptake, metabolism and persistence of racemic, d- and l-methadone were studied in the isolated perfused rabbit lung. The removal of the drug from the perfusate was resolved into two uptake processes (I1 and I2Y. The unidirectional flux of methadone from the perfusate into the lung was linear with respect to perfusate concentration, suggesting an uptake mechanism involving diffusion and/or binding. The uptake of d- and l-methadone was identical. In the absence of methadone in the perfusate, methadone that previously had accumulated in the lung effluxed at three rates (T1/2 = 0.37, 1.65 and 8.9 minutes) suggesting that accumulated methadone was stored in at least three pools (E1, E2, and E3) In addition, a pool with a half-life in excess of 5 hours (noneffuxable pool) was detected; this noneffluxable pool was shown not to be the result of irreversible covalent binding. Methadone was biotransformed to a small extent by the isolated perfused lung to mono- and di-N-demethylated metabolites. Although unchanged methadone and its N-demethylated metabolites were consistently found in the lung at the end of efflux, the mono-N-demethylated metabolite was not always detectable in the perfusate. Nevertheless, the decrease in the rate of efflux of the metabolites with respect to time was identical to that of unchanged methadone.", "contents": "Uptake, metabolism and efflux of methadone in \"single pass\" isolated perfused rabbit lungs. The uptake, metabolism and persistence of racemic, d- and l-methadone were studied in the isolated perfused rabbit lung. The removal of the drug from the perfusate was resolved into two uptake processes (I1 and I2Y. The unidirectional flux of methadone from the perfusate into the lung was linear with respect to perfusate concentration, suggesting an uptake mechanism involving diffusion and/or binding. The uptake of d- and l-methadone was identical. In the absence of methadone in the perfusate, methadone that previously had accumulated in the lung effluxed at three rates (T1/2 = 0.37, 1.65 and 8.9 minutes) suggesting that accumulated methadone was stored in at least three pools (E1, E2, and E3) In addition, a pool with a half-life in excess of 5 hours (noneffuxable pool) was detected; this noneffluxable pool was shown not to be the result of irreversible covalent binding. Methadone was biotransformed to a small extent by the isolated perfused lung to mono- and di-N-demethylated metabolites. Although unchanged methadone and its N-demethylated metabolites were consistently found in the lung at the end of efflux, the mono-N-demethylated metabolite was not always detectable in the perfusate. Nevertheless, the decrease in the rate of efflux of the metabolites with respect to time was identical to that of unchanged methadone.", "PMID": 978490}
{"id": "pubmed23n0032_11755", "title": "Effects of methadone hydrochloride on the growth of organotypic cerebellar cultures prepared from methadone-tolerant and control rats.", "content": "Male and female Sprague-Dawley rats were given dl-methadone (5 mg/kg) for at least 3 months and then mated. The drug was continued throughout pregnancy and after delivery. The newly born pups were divided into two groups. One group was tested for in vivo methadone tolerance, while the animals in the othergroup were used to prepare organotypic cerebellar cultures. Various amounts of dl-methadone were added to the media of half of these cerebellum cultures. The effect of the drug in the medium was assessed by measuring explant outgrowth. Similar experiments were carried out with control animals. Statistical analysis of the data obtained in the in vivo portion of the experiment indicates that the pups of methadone-treated mothers tolerate methadone better than those of untreated mothers. The culture experiments revealed that the addition of methadone to the medium reduced explant outgrowth size and this was a dose-related effect. Also, there was significantly less outgrowth from explants prepared using pups of methadone-treated mothers as compared to the controls. There was no significant difference in the effect of methadone on the growth of cultures prepared from the methadone-tolerant and control animals.", "contents": "Effects of methadone hydrochloride on the growth of organotypic cerebellar cultures prepared from methadone-tolerant and control rats. Male and female Sprague-Dawley rats were given dl-methadone (5 mg/kg) for at least 3 months and then mated. The drug was continued throughout pregnancy and after delivery. The newly born pups were divided into two groups. One group was tested for in vivo methadone tolerance, while the animals in the othergroup were used to prepare organotypic cerebellar cultures. Various amounts of dl-methadone were added to the media of half of these cerebellum cultures. The effect of the drug in the medium was assessed by measuring explant outgrowth. Similar experiments were carried out with control animals. Statistical analysis of the data obtained in the in vivo portion of the experiment indicates that the pups of methadone-treated mothers tolerate methadone better than those of untreated mothers. The culture experiments revealed that the addition of methadone to the medium reduced explant outgrowth size and this was a dose-related effect. Also, there was significantly less outgrowth from explants prepared using pups of methadone-treated mothers as compared to the controls. There was no significant difference in the effect of methadone on the growth of cultures prepared from the methadone-tolerant and control animals.", "PMID": 978491}
{"id": "pubmed23n0032_11756", "title": "Naloxone blockade of morphine analgesia: a dose-effect study of duration and magnitude.", "content": "The purpose of the experiment was to determine the minimal dose of naloxone needed to block the analgesic effect of morphine as measured by the foot-shock titration procedure in the rat. Various groups of rats received, subcutaneously, various doses of naloxone hydrochloride, from 0.03 to 2.0 mg/kg, 15 minutes before receiving 10 mg/kg of morphine sulfate. The results indicate that 1.0 mg/kg of naloxone is the minimum dose necessary to provide full blockade of the measured morphine effect for up to 5 hours after the morphine administration. Partial blockade was evident at doses much smaller than 1.0 mg/kg of naloxone. A dose of 0.125 mg/kg of naloxone completely blocked the onset of a measurable effect from 10 mg/kg of morphine for 60 minutes in six out of the seven animals tested at that dose. The results provide a clear dose-effect relationship for both magnitude and duration of naloxone blockade of the analgesic effect of morphine.", "contents": "Naloxone blockade of morphine analgesia: a dose-effect study of duration and magnitude. The purpose of the experiment was to determine the minimal dose of naloxone needed to block the analgesic effect of morphine as measured by the foot-shock titration procedure in the rat. Various groups of rats received, subcutaneously, various doses of naloxone hydrochloride, from 0.03 to 2.0 mg/kg, 15 minutes before receiving 10 mg/kg of morphine sulfate. The results indicate that 1.0 mg/kg of naloxone is the minimum dose necessary to provide full blockade of the measured morphine effect for up to 5 hours after the morphine administration. Partial blockade was evident at doses much smaller than 1.0 mg/kg of naloxone. A dose of 0.125 mg/kg of naloxone completely blocked the onset of a measurable effect from 10 mg/kg of morphine for 60 minutes in six out of the seven animals tested at that dose. The results provide a clear dose-effect relationship for both magnitude and duration of naloxone blockade of the analgesic effect of morphine.", "PMID": 978492}
{"id": "pubmed23n0032_11757", "title": "Effect of l-propoxyphene on plasma levels and analgesic activity of d-propoxyphene in the rat.", "content": "Oral administration of l-propoxyphene with d-propoxyphene enhances the analgesic activity of d-propoxyphene as expressed in the rat tail heat test. The combination of d- and l-propoxyphene at a dose of 10 mg/kg each was found to have activity in the analgesic assay equivalent to that observed with d-propoxyphene at a dose of 20 mg/kg. In the same test, l-propoxyphene at a dose of 40 mg/kg had no activity. Co-administration of equal amounts of l-propoxyphene with d-propoxyphene (10 mg/kg p.o.) results in an increase in circulating plasma levels of d-propoxyphene from 9 +/- 2 to 114 +/- 39 ng/ml 15 minutes after administration. The increase in plasma levels is accompanied by a proportional increase in the brain and lung levels with no significant change in the liver levels. When d-propoxyphene (4 mug/ml) was infused in the isolated perfused rat liver, over 98% of the drug was extracted in a single pass through the liver. When l-propoxyphene was added to the perfusate (4 mug/ml), the extraction of d-propoxyphene was decreased to less than 90%. These results indicate that l-propoxyphene increases the systemic availability of d-propoxyphene by altering the amount of d-propoxyphene extracted by the liver.", "contents": "Effect of l-propoxyphene on plasma levels and analgesic activity of d-propoxyphene in the rat. Oral administration of l-propoxyphene with d-propoxyphene enhances the analgesic activity of d-propoxyphene as expressed in the rat tail heat test. The combination of d- and l-propoxyphene at a dose of 10 mg/kg each was found to have activity in the analgesic assay equivalent to that observed with d-propoxyphene at a dose of 20 mg/kg. In the same test, l-propoxyphene at a dose of 40 mg/kg had no activity. Co-administration of equal amounts of l-propoxyphene with d-propoxyphene (10 mg/kg p.o.) results in an increase in circulating plasma levels of d-propoxyphene from 9 +/- 2 to 114 +/- 39 ng/ml 15 minutes after administration. The increase in plasma levels is accompanied by a proportional increase in the brain and lung levels with no significant change in the liver levels. When d-propoxyphene (4 mug/ml) was infused in the isolated perfused rat liver, over 98% of the drug was extracted in a single pass through the liver. When l-propoxyphene was added to the perfusate (4 mug/ml), the extraction of d-propoxyphene was decreased to less than 90%. These results indicate that l-propoxyphene increases the systemic availability of d-propoxyphene by altering the amount of d-propoxyphene extracted by the liver.", "PMID": 978493}
{"id": "pubmed23n0032_11758", "title": "Disposition of dobutamine in the dog.", "content": "The disposition of dobutamine, a potent inotropic catecholamine, has been examined in the dog. A sensitive assay has been developed for dobutamine allowing the measurement of the drug at plasma concentrations as low as 1 ng/ml. The short plasma half-life of dobutamine from the plasma to the tissue. When 14C-dobutamine was administered, the plasma half-life of radioactivity was 1.9 hours. The circulating radioactivity consists mainly of the glucuronide conjugate of 3-O-methyldobutamine. During continuous intravenous administration of dobutamine, plasma levels of metabolites reached a maximum between 3 and 4 hours. During a 48-hour time period after administration of 14C-dobutamine, 67% of the radiolable was excreted in the urine and 20% in the feces. In dogs having cannulated bile ducts, 30 to 35% of the administered drug was excreted in the bile. The major urinary metabolites were the glucuronide conjugates of dobutamine and 3-O-methyldobutamine.", "contents": "Disposition of dobutamine in the dog. The disposition of dobutamine, a potent inotropic catecholamine, has been examined in the dog. A sensitive assay has been developed for dobutamine allowing the measurement of the drug at plasma concentrations as low as 1 ng/ml. The short plasma half-life of dobutamine from the plasma to the tissue. When 14C-dobutamine was administered, the plasma half-life of radioactivity was 1.9 hours. The circulating radioactivity consists mainly of the glucuronide conjugate of 3-O-methyldobutamine. During continuous intravenous administration of dobutamine, plasma levels of metabolites reached a maximum between 3 and 4 hours. During a 48-hour time period after administration of 14C-dobutamine, 67% of the radiolable was excreted in the urine and 20% in the feces. In dogs having cannulated bile ducts, 30 to 35% of the administered drug was excreted in the bile. The major urinary metabolites were the glucuronide conjugates of dobutamine and 3-O-methyldobutamine.", "PMID": 978494}
{"id": "pubmed23n0032_11759", "title": "Ionic mechanism of the salicylate block of nerve conduction.", "content": "Benzoic acid, salicylic acid and 5-bromosalicylic acid have been studied for their mechanism of action on crayfish and squid giant axons. External application of 0.1 to 0.5 mM 5-bromosalicylic acid prolonged the falling phase of the action potential, and at concentrations higher than 1.5 mM, nervous conduction was blocked. Boltage clamp analyses showed that 5-bromosalicylic acid reduced both peak transient sodium conductance and steady-state potassium conductance. The curves relating the peak and steady-state conductances to membrane potential were shifted in the direction of depolarization. The time constant for the activation of sodium current (taum) was not affected by 5-bromosalicylic acid, whereas the time constant for the sodium inactivation (tauh) was greatly increased. The steady-state sodium inactivation curve (h infinity) was shifted in the hyperpolarizing direction. The rate constants alpha h and beta h were decreased. The rate of activation of potassium current was reduced. The block of peak transient conductance by externally applied 5-bromosalicylic acid was largely reversed by internal washing suggesting that the drug acts on a membrane site more easily accessible from inside than from outside. Salicylic and benzoic acids were much less potent than 5-bromosalicylic acid, reducing peak conductance by 12.5 and 3%, and steady-state conductance by 8.7 and 7%, respectively, at a concentration of 10 mM.", "contents": "Ionic mechanism of the salicylate block of nerve conduction. Benzoic acid, salicylic acid and 5-bromosalicylic acid have been studied for their mechanism of action on crayfish and squid giant axons. External application of 0.1 to 0.5 mM 5-bromosalicylic acid prolonged the falling phase of the action potential, and at concentrations higher than 1.5 mM, nervous conduction was blocked. Boltage clamp analyses showed that 5-bromosalicylic acid reduced both peak transient sodium conductance and steady-state potassium conductance. The curves relating the peak and steady-state conductances to membrane potential were shifted in the direction of depolarization. The time constant for the activation of sodium current (taum) was not affected by 5-bromosalicylic acid, whereas the time constant for the sodium inactivation (tauh) was greatly increased. The steady-state sodium inactivation curve (h infinity) was shifted in the hyperpolarizing direction. The rate constants alpha h and beta h were decreased. The rate of activation of potassium current was reduced. The block of peak transient conductance by externally applied 5-bromosalicylic acid was largely reversed by internal washing suggesting that the drug acts on a membrane site more easily accessible from inside than from outside. Salicylic and benzoic acids were much less potent than 5-bromosalicylic acid, reducing peak conductance by 12.5 and 3%, and steady-state conductance by 8.7 and 7%, respectively, at a concentration of 10 mM.", "PMID": 978495}
{"id": "pubmed23n0032_11760", "title": "The magnitude and duration of respiratory depression produced by fentanyl and fentanyl plus droperidol in man.", "content": "In 10 healthy male volunteers breathing 100% oxygen, we determined the effect of four intravenous dose levels of fentanyl (0.0015, 0.003, 0.006 and 0.009 mg/kg) and two of fentanyl plus droperidol (i.e., Innovar, 0.003 and 0.006 mg/kg of fentanyl with 2.5 mg of droperidol for each 0.05 mg of fentanyl) on PECO2 and the slope of the ventilatory response to imposed increases in PECO2. All doses of fentanyl and fentanyl plus droperidol depressed the slope and shifted the curve to the right. Depression was dose related and was maximum 5 minutes after administration. The slope returned to control by 2 hours postinjection even at the highest narcotic dose. However, the rightward shift of the CO2 response curve require 4 hours to return to control. Droperidol added to fentanyl did not increase or prolong the respiratory depression seen with fentanyl alone at equivalent dose levels. Nausea and emesis occurred more frequently with fentanyl alone and orthostatic hypotension occurred more frequently with droperidol plus fentanyl. Dysphoria was a prominent consequence of fentanyl plus droperidol administration.", "contents": "The magnitude and duration of respiratory depression produced by fentanyl and fentanyl plus droperidol in man. In 10 healthy male volunteers breathing 100% oxygen, we determined the effect of four intravenous dose levels of fentanyl (0.0015, 0.003, 0.006 and 0.009 mg/kg) and two of fentanyl plus droperidol (i.e., Innovar, 0.003 and 0.006 mg/kg of fentanyl with 2.5 mg of droperidol for each 0.05 mg of fentanyl) on PECO2 and the slope of the ventilatory response to imposed increases in PECO2. All doses of fentanyl and fentanyl plus droperidol depressed the slope and shifted the curve to the right. Depression was dose related and was maximum 5 minutes after administration. The slope returned to control by 2 hours postinjection even at the highest narcotic dose. However, the rightward shift of the CO2 response curve require 4 hours to return to control. Droperidol added to fentanyl did not increase or prolong the respiratory depression seen with fentanyl alone at equivalent dose levels. Nausea and emesis occurred more frequently with fentanyl alone and orthostatic hypotension occurred more frequently with droperidol plus fentanyl. Dysphoria was a prominent consequence of fentanyl plus droperidol administration.", "PMID": 978496}
{"id": "pubmed23n0032_11761", "title": "Co-transport of glycine and sodium across the mucosal border of the midgut epithelium in the marine shrimp, Penaeus marginatus.", "content": "1. Influxes of glycine and Na across the mucosal border of the epithelial cells lining the midgut lumen of a marine shrimp have been examined. 2. Both mucosal JG and JNa displayed Michaelis-Menten kinetics and the influx of each solute was markedly dependent upon the presence of the other solute in the bathing solution. A portion of JG occurred in the absence of Na, but JNa appeared to be completely dependent upon glycine. 3. Glycine influx Kt and Jmax were dependent upon [Na] while Na influx Kt and Jmax depended upon [glycine]. 4. K acted as a competitive inhibitor of Na binding to the co-transport carrier by altering the Kt of glycine influx, but was without significant effect on the amino acid Jmax. 5. The coupling coefficient of mucosal co-transport (JNa/JG) varied with the [Na] being 1-1 at low [Na] (471 mM). 6. A model is proposed in which Na and glycine share ternary (NaXG) and quaternary (Na2XG) carrier complexes during their coupled influxes, but in which glycine may also traverse the mucosal membrane alone by way of an unshared binary (XG) carrier complex.", "contents": "Co-transport of glycine and sodium across the mucosal border of the midgut epithelium in the marine shrimp, Penaeus marginatus. 1. Influxes of glycine and Na across the mucosal border of the epithelial cells lining the midgut lumen of a marine shrimp have been examined. 2. Both mucosal JG and JNa displayed Michaelis-Menten kinetics and the influx of each solute was markedly dependent upon the presence of the other solute in the bathing solution. A portion of JG occurred in the absence of Na, but JNa appeared to be completely dependent upon glycine. 3. Glycine influx Kt and Jmax were dependent upon [Na] while Na influx Kt and Jmax depended upon [glycine]. 4. K acted as a competitive inhibitor of Na binding to the co-transport carrier by altering the Kt of glycine influx, but was without significant effect on the amino acid Jmax. 5. The coupling coefficient of mucosal co-transport (JNa/JG) varied with the [Na] being 1-1 at low [Na] (471 mM). 6. A model is proposed in which Na and glycine share ternary (NaXG) and quaternary (Na2XG) carrier complexes during their coupled influxes, but in which glycine may also traverse the mucosal membrane alone by way of an unshared binary (XG) carrier complex.", "PMID": 978497}
{"id": "pubmed23n0032_11762", "title": "Heat production and chemical change during isometric contraction of rat soleus muscle.", "content": "1. Methods are described whereby the soleus muscle of the rat may be used for the investigation of initial processes in the absence of oxidative recovery. 2. The anaerobic conditions employed had no effect on the concentration of phosphocreatine in resting muscle or the mechanical response during contraction. 3. Muscles were stimulated tetanically for 10 s at 17-18 degrees C. Measurements were made of the heat production and metabolic changes that occurred during a 13 s period following the first stimulus. 4. There was no detectable change in the concentration of ATP. Neither was there detectable activity of adenylate kinase or adenylate deaminase. The changes in the concentration of glycolytic intermediaries were undetectable or very small. 5. The change in the concentration of phosphocreatine was large and amounted to -127 +/- 11-4 mumol/mmol Ct (mean and S.E. of the mean, negative sign indicates break-down, Ct = free creatine + phosphocreatine) which is equivalent to about -2-13 mumol/g wet weight of muscle. The heat production was 6549 +/- 408 mJ/mmol Ct (mean and S.E. of mean) which is equivalent to about 110 mJ/g. 6. About 30% of the observed energy output is unaccounted for by measured metabolic changes. 7. The ratio of heat production (corrected for small amounts of glycolytic activity) to phosphocreatine hydrolysis was -49-7 +/- 5-6 kJ/mol (mean and S.E. of mean), in agreement with previous results using comparable contractions of frog muscle, but different from the enthalpy change associated with phosphocreatine hydrolysis under in vivo conditions (-34 kJ/mol). 8. The results support the notion that the discrepancy between energy output and metabolism is an indication of an unidentified process of substantial energetic significance that is common to a number of species.", "contents": "Heat production and chemical change during isometric contraction of rat soleus muscle. 1. Methods are described whereby the soleus muscle of the rat may be used for the investigation of initial processes in the absence of oxidative recovery. 2. The anaerobic conditions employed had no effect on the concentration of phosphocreatine in resting muscle or the mechanical response during contraction. 3. Muscles were stimulated tetanically for 10 s at 17-18 degrees C. Measurements were made of the heat production and metabolic changes that occurred during a 13 s period following the first stimulus. 4. There was no detectable change in the concentration of ATP. Neither was there detectable activity of adenylate kinase or adenylate deaminase. The changes in the concentration of glycolytic intermediaries were undetectable or very small. 5. The change in the concentration of phosphocreatine was large and amounted to -127 +/- 11-4 mumol/mmol Ct (mean and S.E. of the mean, negative sign indicates break-down, Ct = free creatine + phosphocreatine) which is equivalent to about -2-13 mumol/g wet weight of muscle. The heat production was 6549 +/- 408 mJ/mmol Ct (mean and S.E. of mean) which is equivalent to about 110 mJ/g. 6. About 30% of the observed energy output is unaccounted for by measured metabolic changes. 7. The ratio of heat production (corrected for small amounts of glycolytic activity) to phosphocreatine hydrolysis was -49-7 +/- 5-6 kJ/mol (mean and S.E. of mean), in agreement with previous results using comparable contractions of frog muscle, but different from the enthalpy change associated with phosphocreatine hydrolysis under in vivo conditions (-34 kJ/mol). 8. The results support the notion that the discrepancy between energy output and metabolism is an indication of an unidentified process of substantial energetic significance that is common to a number of species.", "PMID": 978498}
{"id": "pubmed23n0032_11763", "title": "Properties of motor units of the frog sartorius muscle.", "content": "1. The mechanical properties of single motor units in the sartorius muscle of the frog Litoria aurea were examined during single shock and repetitive stimulation of motor axons. 2. The tetanic tension developed by motor units lay in the range 1-40% of whole muscle tension with two peaks in the distribution, in the range 5-10% and 25-30%. The large units had briefer times-to-peak for the twitch than the small units and were more readily fatigued during prolonged repetitive stimulation. 3. Histological examination of the muscle gave a count of 620 muscle fibres with a diameter range of 28-128 mum. Cholinesterase stained preparations showed that the majority of muscle fibres had several nerve terminals (mean 3, range 1-5). 4. Muscle fibres received their multiple innervation from different axons (polyneuronal) or branches of the same axon (multiterminal). The presence of polyneuronal innervation of muscle fibres was confirmed by a comparison of the tensions when each of a pair of motor units was stimulated alone and when they were stimulated together. The tension excess, or overlap, was up to 60% when expressed in terms of the tension developed by either unit alone. Motor units developing similar amounts of tension tended to show more overlap in their innervation than units with very different tensions. 5. An estimate of the amount of multiterminal innervation gave variable results but could account for up to 60% of a motor unit's tension. No correlation could be detected between the values for multiterminal innervation and any other measured parameter. However, it is argued that because of the limitations of the measurements the existence of a relationship between the extent of multiterminal or polyneuronal innervation and the mechanical properties of the motor unit cannot be excluded.", "contents": "Properties of motor units of the frog sartorius muscle. 1. The mechanical properties of single motor units in the sartorius muscle of the frog Litoria aurea were examined during single shock and repetitive stimulation of motor axons. 2. The tetanic tension developed by motor units lay in the range 1-40% of whole muscle tension with two peaks in the distribution, in the range 5-10% and 25-30%. The large units had briefer times-to-peak for the twitch than the small units and were more readily fatigued during prolonged repetitive stimulation. 3. Histological examination of the muscle gave a count of 620 muscle fibres with a diameter range of 28-128 mum. Cholinesterase stained preparations showed that the majority of muscle fibres had several nerve terminals (mean 3, range 1-5). 4. Muscle fibres received their multiple innervation from different axons (polyneuronal) or branches of the same axon (multiterminal). The presence of polyneuronal innervation of muscle fibres was confirmed by a comparison of the tensions when each of a pair of motor units was stimulated alone and when they were stimulated together. The tension excess, or overlap, was up to 60% when expressed in terms of the tension developed by either unit alone. Motor units developing similar amounts of tension tended to show more overlap in their innervation than units with very different tensions. 5. An estimate of the amount of multiterminal innervation gave variable results but could account for up to 60% of a motor unit's tension. No correlation could be detected between the values for multiterminal innervation and any other measured parameter. However, it is argued that because of the limitations of the measurements the existence of a relationship between the extent of multiterminal or polyneuronal innervation and the mechanical properties of the motor unit cannot be excluded.", "PMID": 978499}
{"id": "pubmed23n0032_11764", "title": "The reduced responsiveness of neurones in nucleus reticularis gigantocellularis following their excitation by peripheral nerve stimulation.", "content": "1. Post-stimulus histograms of neuronal activity, constructed from extracellular recordings in decerebrate, decerebellate cats, have been used to investigate the responsiveness of neurons in nucleus reticularis gigantocellularis following their excitation by a peripheral nerve stimulus. 2. The response to a testing stimulus applied to a peripheral nerve was depressed following the response to a conditioning stimulus applied to the same or a different peripheral nerve. This reduction in responsiveness was maximal within 50 msec of the peak of the response to the conditioning stimulus. Response latencies to the testing stimulus were increased during the period of reduced responsiveness. 3. Responsiveness to a peripheral nerve stimulus was also reduced following a spontaneous or an antidromically evoked spike, but this effect was weaker and much shorter-lasting than that following a nerve-evoked spike. Thus, the reduced responsiveness cannot be solely due to phenomena which are an inevitable consequence of an action potential in the neurone. 4. In spontaneously firing neurones, the duration of the reduced responsiveness to a testing stimulus generally outlasted the depression of spontaneous activity which often followed an excitation evoked by a peripheral nerve conditioning stimulus. 5. The reduction in responsiveness to a testing stimulus applied to the same nerve as the conditioning stimulus was greater and longer-lasting than that to a testing stimulus applied to a different nerve. 6. When stimuli were applied to one nerve at a relatively high rate, the neurone became much less responsive to that input, but simultaneously became more responsive to low rate stimulation of other nerves. 7. It is concluded that the greater part of the reduced responsiveness is due to events occurring on the input pathway to a reticular neurone, or possibly in the region of the afferent endings on its dendrites. These processes may allow selective changes in responsiveness to different inputs, and enable the units to act as novelty detectors.", "contents": "The reduced responsiveness of neurones in nucleus reticularis gigantocellularis following their excitation by peripheral nerve stimulation. 1. Post-stimulus histograms of neuronal activity, constructed from extracellular recordings in decerebrate, decerebellate cats, have been used to investigate the responsiveness of neurons in nucleus reticularis gigantocellularis following their excitation by a peripheral nerve stimulus. 2. The response to a testing stimulus applied to a peripheral nerve was depressed following the response to a conditioning stimulus applied to the same or a different peripheral nerve. This reduction in responsiveness was maximal within 50 msec of the peak of the response to the conditioning stimulus. Response latencies to the testing stimulus were increased during the period of reduced responsiveness. 3. Responsiveness to a peripheral nerve stimulus was also reduced following a spontaneous or an antidromically evoked spike, but this effect was weaker and much shorter-lasting than that following a nerve-evoked spike. Thus, the reduced responsiveness cannot be solely due to phenomena which are an inevitable consequence of an action potential in the neurone. 4. In spontaneously firing neurones, the duration of the reduced responsiveness to a testing stimulus generally outlasted the depression of spontaneous activity which often followed an excitation evoked by a peripheral nerve conditioning stimulus. 5. The reduction in responsiveness to a testing stimulus applied to the same nerve as the conditioning stimulus was greater and longer-lasting than that to a testing stimulus applied to a different nerve. 6. When stimuli were applied to one nerve at a relatively high rate, the neurone became much less responsive to that input, but simultaneously became more responsive to low rate stimulation of other nerves. 7. It is concluded that the greater part of the reduced responsiveness is due to events occurring on the input pathway to a reticular neurone, or possibly in the region of the afferent endings on its dendrites. These processes may allow selective changes in responsiveness to different inputs, and enable the units to act as novelty detectors.", "PMID": 978500}
{"id": "pubmed23n0032_11765", "title": "Growth of rats' kidneys after unilateral uretero-caval anastomosis.", "content": "1. The relative significance of reduced excretion of urinary constituents and reduced renal mass, as stimuli to growth of one kidney after the other has been removed, has been investigated. 2. To abolish the excretory function of one kidney without removing it, the right ureter was drained into the vena cava through a compound cannula for 6 weeks. Uretero-caval anastomoses were performed in twenty-four male rats at 10 weeks of age: six survived without evidence of ureteric obstruction (and a further five with minimal obstruction). 3. The rats with anastomoses grew less than six other rats from which the right kidney had been removed or six which had been submitted to a sham operation, and their plasma urea and creatinine concentrations were higher. 4. Relative to body weight, the dry weight of each kidney after uretero-caval anastomosis without obstruction was 18% greater than after sham operation; taking both kidneys together, the total increase was almost as much as in the left kidney alone after right nephrectomy (46%). 5. Histologically and in terms of DNA concentration, the growth of both kidneys after uretero-caval anastomosis was of the same kind as in the left kidney after right nephrectomy. 6. The return of urine from one kidney into the blood provided a powerful stimulus to renal growth in spite of the restraining effect of increased renal mass.", "contents": "Growth of rats' kidneys after unilateral uretero-caval anastomosis. 1. The relative significance of reduced excretion of urinary constituents and reduced renal mass, as stimuli to growth of one kidney after the other has been removed, has been investigated. 2. To abolish the excretory function of one kidney without removing it, the right ureter was drained into the vena cava through a compound cannula for 6 weeks. Uretero-caval anastomoses were performed in twenty-four male rats at 10 weeks of age: six survived without evidence of ureteric obstruction (and a further five with minimal obstruction). 3. The rats with anastomoses grew less than six other rats from which the right kidney had been removed or six which had been submitted to a sham operation, and their plasma urea and creatinine concentrations were higher. 4. Relative to body weight, the dry weight of each kidney after uretero-caval anastomosis without obstruction was 18% greater than after sham operation; taking both kidneys together, the total increase was almost as much as in the left kidney alone after right nephrectomy (46%). 5. Histologically and in terms of DNA concentration, the growth of both kidneys after uretero-caval anastomosis was of the same kind as in the left kidney after right nephrectomy. 6. The return of urine from one kidney into the blood provided a powerful stimulus to renal growth in spite of the restraining effect of increased renal mass.", "PMID": 978501}
{"id": "pubmed23n0032_11766", "title": "Temporal response patterns of the human sinus node to brief carotid baroreceptor stimuli.", "content": "1. A new technique, timed, quantitative neck suction was used to establish in normal man the temporal response patterns of the sinus node to brief baroreceptor stimuli. 2. Maximum sinus node inhibition occurred when stimuli were delivered about 0-75 sec before the anticipated appearance of the subsequent P wave; less pulse interval prolongation occurred when stimuli were delivered earlier, or later in the cardiac cycle. 3. The timing of maximum sinus node responsiveness appeared to be independent of base-line cardiac cycle length. 4. The duration of the baroreceptor-cardiac reflex are (the time from the onset of baroreceptor stimulation until the earliest occurrence of pulse interval prolongation) averaged 0-24 sec. 5. Pulse interval prolongation was proportional to baroreceptor stimulus strength and duration. 6. The results suggest that in man, sinus node responsiveness to baroreceptor stimuli depends critically upon the timing of stimuli within the cardiac cycle.", "contents": "Temporal response patterns of the human sinus node to brief carotid baroreceptor stimuli. 1. A new technique, timed, quantitative neck suction was used to establish in normal man the temporal response patterns of the sinus node to brief baroreceptor stimuli. 2. Maximum sinus node inhibition occurred when stimuli were delivered about 0-75 sec before the anticipated appearance of the subsequent P wave; less pulse interval prolongation occurred when stimuli were delivered earlier, or later in the cardiac cycle. 3. The timing of maximum sinus node responsiveness appeared to be independent of base-line cardiac cycle length. 4. The duration of the baroreceptor-cardiac reflex are (the time from the onset of baroreceptor stimulation until the earliest occurrence of pulse interval prolongation) averaged 0-24 sec. 5. Pulse interval prolongation was proportional to baroreceptor stimulus strength and duration. 6. The results suggest that in man, sinus node responsiveness to baroreceptor stimuli depends critically upon the timing of stimuli within the cardiac cycle.", "PMID": 978502}
{"id": "pubmed23n0032_11767", "title": "Integrated electromyogram and oxygen uptake during positive and negative work.", "content": "1. Integrated electromyogram (e.m.g.) from the vastus lateralis muscles, and steady-state rates of oxygen uptake, were measured simultaneously during the performance of set rates of positive (concentric) and negative (eccentric) work at 50 rev/min on a motorized bicycle ergometer. 2. Similar experiments were also carried out at other pedalling rates and using other leg muscles. 3. The relationships between each of the variables (integrated e.m.g., oxygen consumption) and mean torque on pedals were found to be linear (r greater than 0-98) with a remarkable degree of reproducibility in surface e.m.g. for each subject over several months. 4. The ratio of the e.m.g. slopes at 50 rev/min (positive/negative) was 1-96 +/- 0-12 while the same ratio for the oxygen uptake slopes was 6-34 +/- 0-82. The discrepancy between the ratios suggests that not only is less muscle fibre activity required to maintain the same exerted force during negative work exercise, but there is also a substantial reduction in the oxygen uptake when the fibres are stretched. This was observed for all speeds of pedalling.", "contents": "Integrated electromyogram and oxygen uptake during positive and negative work. 1. Integrated electromyogram (e.m.g.) from the vastus lateralis muscles, and steady-state rates of oxygen uptake, were measured simultaneously during the performance of set rates of positive (concentric) and negative (eccentric) work at 50 rev/min on a motorized bicycle ergometer. 2. Similar experiments were also carried out at other pedalling rates and using other leg muscles. 3. The relationships between each of the variables (integrated e.m.g., oxygen consumption) and mean torque on pedals were found to be linear (r greater than 0-98) with a remarkable degree of reproducibility in surface e.m.g. for each subject over several months. 4. The ratio of the e.m.g. slopes at 50 rev/min (positive/negative) was 1-96 +/- 0-12 while the same ratio for the oxygen uptake slopes was 6-34 +/- 0-82. The discrepancy between the ratios suggests that not only is less muscle fibre activity required to maintain the same exerted force during negative work exercise, but there is also a substantial reduction in the oxygen uptake when the fibres are stretched. This was observed for all speeds of pedalling.", "PMID": 978517}
{"id": "pubmed23n0032_11768", "title": "Spike initiation by transmembrane current: a white-noise analysis.", "content": "1. Those features of a transmembrane current correlated with spike initiation were examined in Aplysia neurones using a Gaussian white-noise stimulus. This stimulus has the advantages that it presents numerous wave forms in random order without prejudgement as to their efficacies, and that it allows straightforward statistical calculations. 2. Stimulation with a repeating segment of Gaussian white-noise current revealed remarkable invariance in the firing times of the tested neurones and indicated a high degree of reliability of their response. 3. Frequencies (less than 5 Hz) involved in spike triggering propagated faithfully for up to several millimetres, justifying intrasomatic current injection to examine spike initiation at the trigger locus. 4. Examination of current wave forms preceding spikes indicated that a wide variety could be effective. Hence, a statistical analysis was performed, including computation of probability densities, averages, standard deviations and correlation coefficients of pairs of current values. Each statistic was displayed as a function of time before the spike. 5. The average current trajectory preceding a spike was multiphasic and depended on the presence and polarity of a d.c. bias. An early relatively small inward- or outward-going phase was followed by a large outward phase before the spike. The early phase tended to oppose the polarity of the d.c. bias. 6. The late outward phase of the average current trajectory reached a maximum 40--75 msec before triggering the action potential (AP) and returned to near zero values at the moment of triggering. The fact that the current peak occurs in advance of the AP may be partially explained by a phase delay between the transmembrane current and potential. The failure of the average current trajectory to return to control values immediately following the peak argues for a positive role of the declining phase in spike triggering. 7. Probability densities preceding spikes were Gaussian, indicating that the average was also the most probable value. Although the densities were broad, confirming that spikes were preceded by a wide variety of current wave forms, their standard deviations were reduced significantly with respect to controls, suggesting preferred status of the average current trajectory in spike triggering. 8. The matrix of correlation coefficients between current pairs suggested that spikes tended to be preceded by wave forms that in part kept close to the average current trajectory and in part preserved its shape. 9. The average first and second derivatives of spike-evoking epochs revealed that current slope and acceleration, respectively, were most crucial in the last 200 msec before spike triggering, and that these dynamic stimulus components were more important for a cell maintained under a depolarizing, rather than a hyperpolarizing bias. 10...", "contents": "Spike initiation by transmembrane current: a white-noise analysis. 1. Those features of a transmembrane current correlated with spike initiation were examined in Aplysia neurones using a Gaussian white-noise stimulus. This stimulus has the advantages that it presents numerous wave forms in random order without prejudgement as to their efficacies, and that it allows straightforward statistical calculations. 2. Stimulation with a repeating segment of Gaussian white-noise current revealed remarkable invariance in the firing times of the tested neurones and indicated a high degree of reliability of their response. 3. Frequencies (less than 5 Hz) involved in spike triggering propagated faithfully for up to several millimetres, justifying intrasomatic current injection to examine spike initiation at the trigger locus. 4. Examination of current wave forms preceding spikes indicated that a wide variety could be effective. Hence, a statistical analysis was performed, including computation of probability densities, averages, standard deviations and correlation coefficients of pairs of current values. Each statistic was displayed as a function of time before the spike. 5. The average current trajectory preceding a spike was multiphasic and depended on the presence and polarity of a d.c. bias. An early relatively small inward- or outward-going phase was followed by a large outward phase before the spike. The early phase tended to oppose the polarity of the d.c. bias. 6. The late outward phase of the average current trajectory reached a maximum 40--75 msec before triggering the action potential (AP) and returned to near zero values at the moment of triggering. The fact that the current peak occurs in advance of the AP may be partially explained by a phase delay between the transmembrane current and potential. The failure of the average current trajectory to return to control values immediately following the peak argues for a positive role of the declining phase in spike triggering. 7. Probability densities preceding spikes were Gaussian, indicating that the average was also the most probable value. Although the densities were broad, confirming that spikes were preceded by a wide variety of current wave forms, their standard deviations were reduced significantly with respect to controls, suggesting preferred status of the average current trajectory in spike triggering. 8. The matrix of correlation coefficients between current pairs suggested that spikes tended to be preceded by wave forms that in part kept close to the average current trajectory and in part preserved its shape. 9. The average first and second derivatives of spike-evoking epochs revealed that current slope and acceleration, respectively, were most crucial in the last 200 msec before spike triggering, and that these dynamic stimulus components were more important for a cell maintained under a depolarizing, rather than a hyperpolarizing bias. 10...", "PMID": 978519}
{"id": "pubmed23n0032_11769", "title": "Changes in electrical properties of rat myometrium during gestation and following hormonal treatments.", "content": "1. The membrane properties of the rat myometrium, during gestation and following ovarian hormone treatment, have been investigated with the micro-electrode technique. 2. Spontaneously generated bursts of electrical activity alternating with silent periods were recorded from non-pregnant, pregnant and post-partum myometria. The membrane potential was highest during the middle stage of gestation, but the spike amplitude within a burst was not uniform. In the final stage of gestation and during parturition, the membrane potential was low and the spikes within a burst were of low frequency and uniform amplitude. 3. During parturition and post-partum, a gradual depolarization of the membrane, accompanied by an increase in membrane resistance, occurred before the generation of a burst. 4. Excitability of the membrane fluctuated from a peak just before the generation of a burst to a low after the cessation of a burst. 5. Displacement of the membrane potential by electrical current or by lowering the temperature modified the slope spontaneous depolarization, but the fluctuations of excitability persisted. The Q10 value for the frequency of spontaneous bursts, measured between 36 and 30 degrees C, was 3-8. 6. Hyperpolarization of the membrane increased the maximum rate of rise of the spike, but beyond -70 mV, the rate of rise was reduced. Half-inactivation of spike generation of spike generation occurred at a membrane potential less negative than the interburst potential, indicating that the current carrying system was not fully activated during parturition. 7. In both normal and spayed rats, oestradiol hyperpolarized the membrane and the burst of spikes was generated hyperpolarized the membrane and the burst of spikes was generated on a sustained depolarization. Progesterone slightly hyperpolarized the membrane and burst discharges occurred without a sustained depolarization. Simultaneous treatment with progesterone and oestradiol produced a plateau potential of long duration during burst discharges. 8. The thickness of the muscle layer, length constant of the tissue and time constant of the membrane were measured during gestation and from spayed rats under various hormonal conditions. The length constant of the tissue was increased by oestradiol and was further increased by simultaneous treatment withoestradiol and progesterone. The increase in tissue thickness appeared to have the most marked influence on the length constant. 9. The resting and active membrane properties of the progresterone treated myometrium were similar to those observed during the middle stages of gestation. The oestradiol-treated myometrium did not resemble that during the last stages of gestation and parturition, which was simulated by combination of the two hormones, oestradiol preceding progesterone.", "contents": "Changes in electrical properties of rat myometrium during gestation and following hormonal treatments. 1. The membrane properties of the rat myometrium, during gestation and following ovarian hormone treatment, have been investigated with the micro-electrode technique. 2. Spontaneously generated bursts of electrical activity alternating with silent periods were recorded from non-pregnant, pregnant and post-partum myometria. The membrane potential was highest during the middle stage of gestation, but the spike amplitude within a burst was not uniform. In the final stage of gestation and during parturition, the membrane potential was low and the spikes within a burst were of low frequency and uniform amplitude. 3. During parturition and post-partum, a gradual depolarization of the membrane, accompanied by an increase in membrane resistance, occurred before the generation of a burst. 4. Excitability of the membrane fluctuated from a peak just before the generation of a burst to a low after the cessation of a burst. 5. Displacement of the membrane potential by electrical current or by lowering the temperature modified the slope spontaneous depolarization, but the fluctuations of excitability persisted. The Q10 value for the frequency of spontaneous bursts, measured between 36 and 30 degrees C, was 3-8. 6. Hyperpolarization of the membrane increased the maximum rate of rise of the spike, but beyond -70 mV, the rate of rise was reduced. Half-inactivation of spike generation of spike generation occurred at a membrane potential less negative than the interburst potential, indicating that the current carrying system was not fully activated during parturition. 7. In both normal and spayed rats, oestradiol hyperpolarized the membrane and the burst of spikes was generated hyperpolarized the membrane and the burst of spikes was generated on a sustained depolarization. Progesterone slightly hyperpolarized the membrane and burst discharges occurred without a sustained depolarization. Simultaneous treatment with progesterone and oestradiol produced a plateau potential of long duration during burst discharges. 8. The thickness of the muscle layer, length constant of the tissue and time constant of the membrane were measured during gestation and from spayed rats under various hormonal conditions. The length constant of the tissue was increased by oestradiol and was further increased by simultaneous treatment withoestradiol and progesterone. The increase in tissue thickness appeared to have the most marked influence on the length constant. 9. The resting and active membrane properties of the progresterone treated myometrium were similar to those observed during the middle stages of gestation. The oestradiol-treated myometrium did not resemble that during the last stages of gestation and parturition, which was simulated by combination of the two hormones, oestradiol preceding progesterone.", "PMID": 978524}
{"id": "pubmed23n0032_11770", "title": "Effects of prostaglandin E2 and oxytocin on the electrical activity of hormone-treated and pregnant rat myometria.", "content": "The effects of prostaglandin E2 (PGE2) and oxytocin on the electrical activity of rat myometrium at various stages of gestation and following hormonal treatment have been investigated. 1. PGE2 and oxytocin produce an excitation of the myometrial membrane under all experimental conditions. The sensitivity of the myometrium markedly increases during the last stage of gestation, and at parturition is more than one thousand times greater than in the mid-pregnant rat. The sensitivity of the myometrium to oxytocin increases rapidly during the last stage of gestation but to PGE2 the increase is gradual, beginning in the late middle stage of gestation. 2. During the early middle stage of gestation, the sensitivity of the myometrium to PGE2 and oxytocin is lower than in the non-pregnant myometrium. 3. After oestradiol treatment, the sensitivity to PGE2 and oxytocin increases but the sensitivity is much weaker than that during the parturition. On the other hand, after progesterone treatment, the sensitivity is reduced below that of the castrated rat. 4. Differences in the sensitivity of progesterone-treated and oestradiol-treated myometria to PGE2 and oxytocin are compared to those of the pregnant and post-partum myometrium. The results show that the sensitivity of the myometrium to PGE2 and oxytocin during the early and early-middle stages of gestation can be simulated by progesterone treatment, but that the sensitivity during the last stage of gestation and during parturition cannot be simulated by oestradiol and progesterone treatment.", "contents": "Effects of prostaglandin E2 and oxytocin on the electrical activity of hormone-treated and pregnant rat myometria. The effects of prostaglandin E2 (PGE2) and oxytocin on the electrical activity of rat myometrium at various stages of gestation and following hormonal treatment have been investigated. 1. PGE2 and oxytocin produce an excitation of the myometrial membrane under all experimental conditions. The sensitivity of the myometrium markedly increases during the last stage of gestation, and at parturition is more than one thousand times greater than in the mid-pregnant rat. The sensitivity of the myometrium to oxytocin increases rapidly during the last stage of gestation but to PGE2 the increase is gradual, beginning in the late middle stage of gestation. 2. During the early middle stage of gestation, the sensitivity of the myometrium to PGE2 and oxytocin is lower than in the non-pregnant myometrium. 3. After oestradiol treatment, the sensitivity to PGE2 and oxytocin increases but the sensitivity is much weaker than that during the parturition. On the other hand, after progesterone treatment, the sensitivity is reduced below that of the castrated rat. 4. Differences in the sensitivity of progesterone-treated and oestradiol-treated myometria to PGE2 and oxytocin are compared to those of the pregnant and post-partum myometrium. The results show that the sensitivity of the myometrium to PGE2 and oxytocin during the early and early-middle stages of gestation can be simulated by progesterone treatment, but that the sensitivity during the last stage of gestation and during parturition cannot be simulated by oestradiol and progesterone treatment.", "PMID": 978527}
{"id": "pubmed23n0032_11771", "title": "The connexion between the ion-binding sites of the sodium pump.", "content": "1. A study has been made of K influx into human red blood cells in order to determine the influence of internal Na on the affinity of the Na pump for external K. Cells were prepared to contain minimal K and two Na concentrations (about 10 and 30 mueqiv/ml. cells) and incubated in solutions with a range of K concentrations. 2. In choline (Na-free) Ringer, activation of K influx by external K was hyperbolic. The Km for external K increased as the internal Na concentration was raised. The increase was greater than the increase in K influx with saturating external K. The Km for external K increased towards a limiting value as the internal Na concentration was raised. 3. In contrast, in Na-Ringer, the activation by external K was sigmoidal and the affinity for external K was independent of the internal Na concentration. 4. K influx was measured at two submaximal levels of external K with a range of internal Na. The affinity for internal Na fell as external K was raised. 5. The results suggest that in the absence of competition between Na and K on the same side of the membrane, there is a connexion between the ion-binding sites of the Na pump.", "contents": "The connexion between the ion-binding sites of the sodium pump. 1. A study has been made of K influx into human red blood cells in order to determine the influence of internal Na on the affinity of the Na pump for external K. Cells were prepared to contain minimal K and two Na concentrations (about 10 and 30 mueqiv/ml. cells) and incubated in solutions with a range of K concentrations. 2. In choline (Na-free) Ringer, activation of K influx by external K was hyperbolic. The Km for external K increased as the internal Na concentration was raised. The increase was greater than the increase in K influx with saturating external K. The Km for external K increased towards a limiting value as the internal Na concentration was raised. 3. In contrast, in Na-Ringer, the activation by external K was sigmoidal and the affinity for external K was independent of the internal Na concentration. 4. K influx was measured at two submaximal levels of external K with a range of internal Na. The affinity for internal Na fell as external K was raised. 5. The results suggest that in the absence of competition between Na and K on the same side of the membrane, there is a connexion between the ion-binding sites of the Na pump.", "PMID": 978531}
{"id": "pubmed23n0032_11772", "title": "Joint sense, muscle sense, and their combination as position sense, measured at the distal interphalangeal joint of the middle finger.", "content": "1. An anatomical peculiarity allows the hand to be positioned so that the terminal phalanx of the middle finger cannot be moved by voluntary effort. When positioned in this way only joint and cutaneous mechanisms subserve position sense. By altering the position of the hand the muscles are again engaged and able to move the finger. Moving the joint then also excites muscular afferents. 2. The position sense of twelve subjects was assessed with and without engagement of the muscles at the joint. Three tests were used in which either angular displacement, angular velocity or duration of displacement were held constant. 3. When muscular attachment was restored, performance in all tests was greatly enhanced. As engagement of the muscles caused little change in the 'stiffness' of the joint, it is unlikely that the improved performance resulted from increased discharges from the joint receptors. Cutaneous mechanisms are unlikely to mediate this improvement as they are likely to have been unaffected by engagement of muscles. It is concluded that intramuscular receptors are partly responsible for normal position sense. 4. In seven of the twelve subjects the test finger was anaesthetized to isolate the contribution of intramuscular receptors. This muscle sense was variable. In some subjects it provided accurate kinaesthetic information but in others the information was crude. If with the test finger anaesthetized subjects exerted voluntary tension with the muscles that move the joint the muscle sense was improved.", "contents": "Joint sense, muscle sense, and their combination as position sense, measured at the distal interphalangeal joint of the middle finger. 1. An anatomical peculiarity allows the hand to be positioned so that the terminal phalanx of the middle finger cannot be moved by voluntary effort. When positioned in this way only joint and cutaneous mechanisms subserve position sense. By altering the position of the hand the muscles are again engaged and able to move the finger. Moving the joint then also excites muscular afferents. 2. The position sense of twelve subjects was assessed with and without engagement of the muscles at the joint. Three tests were used in which either angular displacement, angular velocity or duration of displacement were held constant. 3. When muscular attachment was restored, performance in all tests was greatly enhanced. As engagement of the muscles caused little change in the 'stiffness' of the joint, it is unlikely that the improved performance resulted from increased discharges from the joint receptors. Cutaneous mechanisms are unlikely to mediate this improvement as they are likely to have been unaffected by engagement of muscles. It is concluded that intramuscular receptors are partly responsible for normal position sense. 4. In seven of the twelve subjects the test finger was anaesthetized to isolate the contribution of intramuscular receptors. This muscle sense was variable. In some subjects it provided accurate kinaesthetic information but in others the information was crude. If with the test finger anaesthetized subjects exerted voluntary tension with the muscles that move the joint the muscle sense was improved.", "PMID": 978533}
{"id": "pubmed23n0032_11773", "title": "Lung inflation and alveolar permeability to non-electrolytes in the adult sheep in vivo.", "content": "1. Experiments were performed on adult sheep to determine the effect of lung distension on the passive permeability of alveoli to water soluble non-electrolytes. With the animal breathing oxygen spontaneously, a segment of one lung was isolated by passing a balloon-tipped catheter through a tracheostomy into a distal bronchus. This isolated atelectatic segment was filled with an isosomotic saline solution containing radio-labelled solutes of known molecular size: [125I]albumin, [14C]inulin, [14C]-sucrose, [3H]mannitol, and [14C]urea. The segment was inflated with oxygen either to a preselected pressure, or to a predetermined fraction of its capacity. Inflation was then maintained for several 10-15 min periods between which the oxygen supply was disconnected and the saline sampled, allowing the tracer concentrations to be measured. 2. At low inflating pressures (20-32 cmH2O) and at low volumes (24-54% of capacity), alveolar permeability to water soluble solutes was slight and could be characterized in terms of a membrane penetrated by cylindrical water filled pores of 0-5-1-6 nm radius. In all experiments showing restricted diffusion, absorption of saline occurred. 3. There was a positive correlation between the degree of lung inflation and pore radius in both the pressure controlled and volume controlled experiments. At high inflating pressures and at inflation volumes which were close to the total capacity of the isolated segment, restriction of solute diffusion was lost; in five out of six such experiments there was a net movement of liquid into the alveoli. 4. These results can be explained by postulating that as the lung epithelium is progressively stretched there is an opening up of water filled channels between alveolar cells. At peak inflation, restriction of diffusion of water soluble solutes is lost, and the alveolar epithelium ceases to function as a barrier between the circulation and air spaces.", "contents": "Lung inflation and alveolar permeability to non-electrolytes in the adult sheep in vivo. 1. Experiments were performed on adult sheep to determine the effect of lung distension on the passive permeability of alveoli to water soluble non-electrolytes. With the animal breathing oxygen spontaneously, a segment of one lung was isolated by passing a balloon-tipped catheter through a tracheostomy into a distal bronchus. This isolated atelectatic segment was filled with an isosomotic saline solution containing radio-labelled solutes of known molecular size: [125I]albumin, [14C]inulin, [14C]-sucrose, [3H]mannitol, and [14C]urea. The segment was inflated with oxygen either to a preselected pressure, or to a predetermined fraction of its capacity. Inflation was then maintained for several 10-15 min periods between which the oxygen supply was disconnected and the saline sampled, allowing the tracer concentrations to be measured. 2. At low inflating pressures (20-32 cmH2O) and at low volumes (24-54% of capacity), alveolar permeability to water soluble solutes was slight and could be characterized in terms of a membrane penetrated by cylindrical water filled pores of 0-5-1-6 nm radius. In all experiments showing restricted diffusion, absorption of saline occurred. 3. There was a positive correlation between the degree of lung inflation and pore radius in both the pressure controlled and volume controlled experiments. At high inflating pressures and at inflation volumes which were close to the total capacity of the isolated segment, restriction of solute diffusion was lost; in five out of six such experiments there was a net movement of liquid into the alveoli. 4. These results can be explained by postulating that as the lung epithelium is progressively stretched there is an opening up of water filled channels between alveolar cells. At peak inflation, restriction of diffusion of water soluble solutes is lost, and the alveolar epithelium ceases to function as a barrier between the circulation and air spaces.", "PMID": 978536}
{"id": "pubmed23n0032_11774", "title": "Inhibition and facilitation of antidromically identified tubero-infundibular neurones following stimulation of the median eminence in the rat.", "content": "1. Stimulation of the median eminence of female rats inhibited the spontaneous firing of antidromically identified tubero-infundibular units. Some units could be inhibited by stimuli subthreshold for the antidromic spike. A conditioning stimulus of subthreshold intensity for the antidromic spike also delayed or abolished the invasion of the soma and dendrites of the same unit by antidromic action potentials evoked by a suprathreshold stimulus given 2-10 msec later. 2. Although strychnine (0-2 mg/kg, i.v.) did not significantly alter the inhibition evoked by stimulation of the median eminence, it was abolished by picrotoxin (2-6 mg/kg, iv.). 3. In seven of the fifty-seven identified units examined stimulation of the median eminence facilitated the spontaneous firing. However, after an i.v. injection of picrotoxin the facilitory response was observed in thirty-seven of the forty-six units tested. Post-stimulus time histograms obtained from the same unit before and after an injection of picrotoxin demonstrated that the latency and duration of the facilitation did not always coincide with that of the inhibition. 4. After an injection of picrotoxin the field potential evoked by antidromic stimulation of the median eminence consisted of a small positive wave followed by a negative wave. Frequently the negative wave of the field potential was accompanied by a convulsive discharge. The latency of the negative wave appears to be identical to that of the facilitation seen in nearby single units. 5. The facilitation evoked by antidromic stimulation in the presence of picrotoxin was blocked by an i.v. injection of alpha-methyl-p-tyrosine (250 or 375 mg/kg). None of the nine units sampled from rats pre-treated with alpha-methyl-p-tyrosine injected twice I.P. (250 mg/kg for each) were facilitated by stimulation of the median eminence following I.V. picrotoxin, while eight of the eleven units sampled from control rats pre-treated with L-tyrosine could be facilitated by antidromic stimulation. 6. These results suggest that tuber-infundibular neurosecretory neurones are inhibited and facilitated by neural pathways which could involve the axon collaterals of the neruosecretory neurones which project to the external layer of the median eminence. It is also suggested that GABA-releasing neurones mediate the inhibition and catecholaminergic neurones are involved in the facilitory pathways. Presumably the facilitation is normally masked by the activity of the GABA-releasing neurones.", "contents": "Inhibition and facilitation of antidromically identified tubero-infundibular neurones following stimulation of the median eminence in the rat. 1. Stimulation of the median eminence of female rats inhibited the spontaneous firing of antidromically identified tubero-infundibular units. Some units could be inhibited by stimuli subthreshold for the antidromic spike. A conditioning stimulus of subthreshold intensity for the antidromic spike also delayed or abolished the invasion of the soma and dendrites of the same unit by antidromic action potentials evoked by a suprathreshold stimulus given 2-10 msec later. 2. Although strychnine (0-2 mg/kg, i.v.) did not significantly alter the inhibition evoked by stimulation of the median eminence, it was abolished by picrotoxin (2-6 mg/kg, iv.). 3. In seven of the fifty-seven identified units examined stimulation of the median eminence facilitated the spontaneous firing. However, after an i.v. injection of picrotoxin the facilitory response was observed in thirty-seven of the forty-six units tested. Post-stimulus time histograms obtained from the same unit before and after an injection of picrotoxin demonstrated that the latency and duration of the facilitation did not always coincide with that of the inhibition. 4. After an injection of picrotoxin the field potential evoked by antidromic stimulation of the median eminence consisted of a small positive wave followed by a negative wave. Frequently the negative wave of the field potential was accompanied by a convulsive discharge. The latency of the negative wave appears to be identical to that of the facilitation seen in nearby single units. 5. The facilitation evoked by antidromic stimulation in the presence of picrotoxin was blocked by an i.v. injection of alpha-methyl-p-tyrosine (250 or 375 mg/kg). None of the nine units sampled from rats pre-treated with alpha-methyl-p-tyrosine injected twice I.P. (250 mg/kg for each) were facilitated by stimulation of the median eminence following I.V. picrotoxin, while eight of the eleven units sampled from control rats pre-treated with L-tyrosine could be facilitated by antidromic stimulation. 6. These results suggest that tuber-infundibular neurosecretory neurones are inhibited and facilitated by neural pathways which could involve the axon collaterals of the neruosecretory neurones which project to the external layer of the median eminence. It is also suggested that GABA-releasing neurones mediate the inhibition and catecholaminergic neurones are involved in the facilitory pathways. Presumably the facilitation is normally masked by the activity of the GABA-releasing neurones.", "PMID": 978540}
{"id": "pubmed23n0032_11775", "title": "The interaction between cutaneous and spinal therman inputs in the control of oxygen consumption in the rat.", "content": "1. The effect of thermal stimulation of the spinal cord on the rate of oxygen consumption was studied in five unanaesthetized rats during exposure to various ambient temperatures. 2. In a warm environment, cooling the spinal cord had no effect on the rate of oxygen consumption but in thermoneutral and cold environments the rate of oxygen consumption increased proportionally to the intensity of spinal cooling. Heating the spinal cord decreased the level of oxygen consumption and, if intense enough, suppressed the thermoregulatory increase in metabolic rate. 3. It is concluded that, in the control of oxygen consumption in the rat, the afferent signals from thermal sensors in the spinal cord and skin are added.", "contents": "The interaction between cutaneous and spinal therman inputs in the control of oxygen consumption in the rat. 1. The effect of thermal stimulation of the spinal cord on the rate of oxygen consumption was studied in five unanaesthetized rats during exposure to various ambient temperatures. 2. In a warm environment, cooling the spinal cord had no effect on the rate of oxygen consumption but in thermoneutral and cold environments the rate of oxygen consumption increased proportionally to the intensity of spinal cooling. Heating the spinal cord decreased the level of oxygen consumption and, if intense enough, suppressed the thermoregulatory increase in metabolic rate. 3. It is concluded that, in the control of oxygen consumption in the rat, the afferent signals from thermal sensors in the spinal cord and skin are added.", "PMID": 978543}
{"id": "pubmed23n0032_11776", "title": "Possible presynaptic inhibition in rat olfactory cortex.", "content": "1. Field potentials were evoked from rat olfactory cortex slices in vitro at room temperature by lateral olfactory tract stimulation. At very slow stimulation rates (less than 0-1 Hz) a delayed negative wave (late N-wave) was found to follow the first negative wave (N-wave). This late N-wave started 30 msec after the stimulus and lasted for 50-150 msec. 2. Low Ca2+/high Mg2+ medium abolished the late N-wave more rapidly than the N-wave, suggesting a possible multisynaptic origin. 3. The GABA antagonist bicuculline (10(-6) M) abolished the late N-wave without affecting the N-wave. 4. During the late N-wave, both the tract action potential and the N-wave to a second stimulus were reduced. This was attributed, at least in part, to collision with antidromic action potentials which could be detected during the late N-wave. 5. These findings are discussed in terms of a possible presynaptic depolarizing action of a GABA-like transmitter giving rise to presynaptic inhibition and the late N-wave.", "contents": "Possible presynaptic inhibition in rat olfactory cortex. 1. Field potentials were evoked from rat olfactory cortex slices in vitro at room temperature by lateral olfactory tract stimulation. At very slow stimulation rates (less than 0-1 Hz) a delayed negative wave (late N-wave) was found to follow the first negative wave (N-wave). This late N-wave started 30 msec after the stimulus and lasted for 50-150 msec. 2. Low Ca2+/high Mg2+ medium abolished the late N-wave more rapidly than the N-wave, suggesting a possible multisynaptic origin. 3. The GABA antagonist bicuculline (10(-6) M) abolished the late N-wave without affecting the N-wave. 4. During the late N-wave, both the tract action potential and the N-wave to a second stimulus were reduced. This was attributed, at least in part, to collision with antidromic action potentials which could be detected during the late N-wave. 5. These findings are discussed in terms of a possible presynaptic depolarizing action of a GABA-like transmitter giving rise to presynaptic inhibition and the late N-wave.", "PMID": 978545}
{"id": "pubmed23n0032_11777", "title": "Conduction velocity along the afferent vagal dendrites: a new type of fibre.", "content": "1. We systematically calculated the conduction velocity along the peripheral extensions of sensory vagal neurones in cats (the dendrites). In addition, a study of excitability cycle and light microscopic investigation were also conducted on these neurones. 2. The conduction velocity of the three known types of fibres (A, B and C) remains uniform along the dendrites. 3. Another mixed type of fibres exists with a C conduction velocity (mean value 1-5 m/sec) along its distal pathway and a B conduction velocity (mean value 6 m/sec) along its proximal pathway. The change in conduction velocity progressively occurs in the thoraco-cervical portion of the vagus nerve at least 20 mm from the receptor and at least 40 mm from the T cell. 4. The mixed fibres exhibited a C type excitability cycle in their peripheral pathway and a B type excitability cycle in their central pathway. 5. The histological study using the teasing method demonstrated the existence of unmyelinated fibres, in the thoraco-cervical region of the vagus nerve, becoming progressively myelinated from the periphery to the nodose ganglion. These fibres are likely to be the ones showing mixed electrophysiological properties. They represent (approximately) 10% of the vagal nerve population. 6. We propose to call the mixed fibres BC because they present electrophysiological and morphological properties of C fibres in their distal part and properties of B fibres in their proximal part.", "contents": "Conduction velocity along the afferent vagal dendrites: a new type of fibre. 1. We systematically calculated the conduction velocity along the peripheral extensions of sensory vagal neurones in cats (the dendrites). In addition, a study of excitability cycle and light microscopic investigation were also conducted on these neurones. 2. The conduction velocity of the three known types of fibres (A, B and C) remains uniform along the dendrites. 3. Another mixed type of fibres exists with a C conduction velocity (mean value 1-5 m/sec) along its distal pathway and a B conduction velocity (mean value 6 m/sec) along its proximal pathway. The change in conduction velocity progressively occurs in the thoraco-cervical portion of the vagus nerve at least 20 mm from the receptor and at least 40 mm from the T cell. 4. The mixed fibres exhibited a C type excitability cycle in their peripheral pathway and a B type excitability cycle in their central pathway. 5. The histological study using the teasing method demonstrated the existence of unmyelinated fibres, in the thoraco-cervical region of the vagus nerve, becoming progressively myelinated from the periphery to the nodose ganglion. These fibres are likely to be the ones showing mixed electrophysiological properties. They represent (approximately) 10% of the vagal nerve population. 6. We propose to call the mixed fibres BC because they present electrophysiological and morphological properties of C fibres in their distal part and properties of B fibres in their proximal part.", "PMID": 978547}
{"id": "pubmed23n0032_11778", "title": "Increment sensitivity in humans with abnormal visual experience.", "content": "1. Visual acuity is lower for gratings oriented diagonally than for those of horizontal and vertical orientations. In addition to this oblique effect, some subjects show substantial deficits in acuity for horizontal or vertical targets (meridional amblyopia). These subjects are invariably astigmatic, but the condition has a neuronal basis and is thought to arise from faulty post-natal neural development. 2. Foveal increment sensitivites have been determined for normal subjects and meridional amblyopes using bar-shaped targets of various lengths, widths and orientations.3. Normal subjects do not exhibit differences in sensitivity as a function of orientation. No oblique effect is found for 1-5' wide bars ranging in length from 10 to 60'. On the other hand, meridional amblyopes have substantial differences in increment sensitivity which depend on test target orientation. Invariably, when there is a deficit in acuity for a particular grating orientation, there is also a reduction in increment sensitivity for a bar of the same orientation. This effect is diminshed or eliminated when the background illuminance is lowered from 70 to 7td. 4. The orientational differences in increment sensitivity found in meridional amblyopes do not increases for bars longer than about 10'. As the bar is shortened, the differences are reduced, and they are absent when the test bar is 6' or less. 5. In normal subjects, for a 1 degree long bar, increment sensitivity increases with width up to about 4' where the width-sensitivity curve levels off. No orientation differences are exhibited. Prominent orientation differences are found with meridional amblyopes when the bar target width is altered. The normal meridian is similar to those of the control subjects but the deficity meridian has very low sensitivity and summation is present for widths up to about 11'.", "contents": "Increment sensitivity in humans with abnormal visual experience. 1. Visual acuity is lower for gratings oriented diagonally than for those of horizontal and vertical orientations. In addition to this oblique effect, some subjects show substantial deficits in acuity for horizontal or vertical targets (meridional amblyopia). These subjects are invariably astigmatic, but the condition has a neuronal basis and is thought to arise from faulty post-natal neural development. 2. Foveal increment sensitivites have been determined for normal subjects and meridional amblyopes using bar-shaped targets of various lengths, widths and orientations.3. Normal subjects do not exhibit differences in sensitivity as a function of orientation. No oblique effect is found for 1-5' wide bars ranging in length from 10 to 60'. On the other hand, meridional amblyopes have substantial differences in increment sensitivity which depend on test target orientation. Invariably, when there is a deficit in acuity for a particular grating orientation, there is also a reduction in increment sensitivity for a bar of the same orientation. This effect is diminshed or eliminated when the background illuminance is lowered from 70 to 7td. 4. The orientational differences in increment sensitivity found in meridional amblyopes do not increases for bars longer than about 10'. As the bar is shortened, the differences are reduced, and they are absent when the test bar is 6' or less. 5. In normal subjects, for a 1 degree long bar, increment sensitivity increases with width up to about 4' where the width-sensitivity curve levels off. No orientation differences are exhibited. Prominent orientation differences are found with meridional amblyopes when the bar target width is altered. The normal meridian is similar to those of the control subjects but the deficity meridian has very low sensitivity and summation is present for widths up to about 11'.", "PMID": 978567}
{"id": "pubmed23n0032_11779", "title": "Identification of pathways mediating cardiovascular responses elicited by stimulation of the septum in the rat.", "content": "1. Experiments were done in rats anaesthetized with sodium pentobarbitone to localize pathways mediating the cardiovascular responses elicited by electrical stimulation of the septum. The major efferent projections from the septum were first identified anatomically by the Fink-Heimer II technique and these pathways were subsequently lesioned in acute experiments to establish their role in the mediation of the cardiovascular responses elicited by septal stimulation. 2. Electrical stimulation of histologically localized sites in the lateral septum elicited hypotension and bradycardia whereas stimulation of sites in the medial septum elicited hypertension and bradycardia. 3. Selective lesions of cardiovascular responsive sites in either the lateral or medial septum produced a pattern of degeneration essentially similar to that of previous anatomical studies, i.e. the main efferent projections were localized to the fornix, stria medullaris and the medial forebrain bundle. 4. Stimulation of the fornix did not elicit cardiovascular changes and lesions of this pathway did not alter cardiovascular responses to septal stimulation. 5. Stimulation of the stria medullaris elicited hypotension and bradycardia whereas stimulation of the medial forebrain bundle elicited hypertension and bradycardia. Ipsilateral lesions of the stria medullaris, signicicantly attenuated the hypotension and bradycardia elicited by stimulation of the lateral septum but did not affect the responses to stimulation of the medial septum. Bilateral lesions of the medial forebrain bundle in the region of the lateral hypothalamus abolished the hypertension but did not affect the bradycardia elicited by medial septum stimulation. Cardiovascular, reponses elicited by stimulation of the lateral septum were not affected by medial forebrain bundle lesions. 6. It is suggested that the cardiovascular responses elicited by stimulation of the lateral septum are mediated via the stria medullaris and that the hypertension elicited by stimulation of the medial septum is mediated via the medial forebrain bundle. On the other hand, the bradycardia elicited by stimulation of the medial septum is probably mediated by a pathway presently unknown.", "contents": "Identification of pathways mediating cardiovascular responses elicited by stimulation of the septum in the rat. 1. Experiments were done in rats anaesthetized with sodium pentobarbitone to localize pathways mediating the cardiovascular responses elicited by electrical stimulation of the septum. The major efferent projections from the septum were first identified anatomically by the Fink-Heimer II technique and these pathways were subsequently lesioned in acute experiments to establish their role in the mediation of the cardiovascular responses elicited by septal stimulation. 2. Electrical stimulation of histologically localized sites in the lateral septum elicited hypotension and bradycardia whereas stimulation of sites in the medial septum elicited hypertension and bradycardia. 3. Selective lesions of cardiovascular responsive sites in either the lateral or medial septum produced a pattern of degeneration essentially similar to that of previous anatomical studies, i.e. the main efferent projections were localized to the fornix, stria medullaris and the medial forebrain bundle. 4. Stimulation of the fornix did not elicit cardiovascular changes and lesions of this pathway did not alter cardiovascular responses to septal stimulation. 5. Stimulation of the stria medullaris elicited hypotension and bradycardia whereas stimulation of the medial forebrain bundle elicited hypertension and bradycardia. Ipsilateral lesions of the stria medullaris, signicicantly attenuated the hypotension and bradycardia elicited by stimulation of the lateral septum but did not affect the responses to stimulation of the medial septum. Bilateral lesions of the medial forebrain bundle in the region of the lateral hypothalamus abolished the hypertension but did not affect the bradycardia elicited by medial septum stimulation. Cardiovascular, reponses elicited by stimulation of the lateral septum were not affected by medial forebrain bundle lesions. 6. It is suggested that the cardiovascular responses elicited by stimulation of the lateral septum are mediated via the stria medullaris and that the hypertension elicited by stimulation of the medial septum is mediated via the medial forebrain bundle. On the other hand, the bradycardia elicited by stimulation of the medial septum is probably mediated by a pathway presently unknown.", "PMID": 978568}
{"id": "pubmed23n0032_11780", "title": "Lithium protection against oxygen toxicity in rats: ammonia and amino acid metabolism.", "content": "1. The use of Li pre-treatment in rats before high pressure oxygen exposure has been reported effective in controlling convulsions. This is an effect which is better demonstrated if exposure to oxygen follows shortly after Li injection than exposure following several hours later. 2. This study has investigated the hypothesis that the protective action of Li may be exerted, in the short term, by its removing ammonia from the blood and alleviating the latter's known toxic action. 3. A normal Li distribution time profile in unstressed rat brain and blood following intraperitoneal injection has been established. Brain and blood ammonia, amino acids and Li concentrations were also measured in Li-treated animals exposed and convulsed by oxygen. These measurements were made both shortly (15 min) and also several hours after (24 hr) Li treatment. Ammonia and amino acid values in Li-protected groups were compared to normal unstressed animal values and also to values in animals convulsed by oxygen unprotected by Li pre-treatment. 4. In rat brain abd blood significant (P less than 0-001) elevation of ammonia and glutamine and depression of gamma-amino butyric acid (brain only) and glutamate was noted following oxygen treatment in unprotected animals. Prior injection of Li 15 min before high pressure oxygen exposure delayed convulsions twice as long. Additionally if these animals were only exposed to oxygen for a period of time equal to that which would normally produce convulsions in unprotected animals, brain and blood ammonia and amino acids were maintained near to unstressed animal levels. Concomitantly, blood Li concentrations were considerably depressed below the values one would expect from the previously determined Li distribution time profile. 5. In rats exposed to high pressure oxygen 24 hr after Li treatment there was no protective action against high pressure oxygen convulsion, rather a potentiating effect for convulsion was seen. 6. These data present compelling evidence for the controlling effect of Li in rats, on rising blood ammonia concentration which occurs in high pressure oxygen exposure. The effect might well be due to the known chelating properties of Li with ammonia.", "contents": "Lithium protection against oxygen toxicity in rats: ammonia and amino acid metabolism. 1. The use of Li pre-treatment in rats before high pressure oxygen exposure has been reported effective in controlling convulsions. This is an effect which is better demonstrated if exposure to oxygen follows shortly after Li injection than exposure following several hours later. 2. This study has investigated the hypothesis that the protective action of Li may be exerted, in the short term, by its removing ammonia from the blood and alleviating the latter's known toxic action. 3. A normal Li distribution time profile in unstressed rat brain and blood following intraperitoneal injection has been established. Brain and blood ammonia, amino acids and Li concentrations were also measured in Li-treated animals exposed and convulsed by oxygen. These measurements were made both shortly (15 min) and also several hours after (24 hr) Li treatment. Ammonia and amino acid values in Li-protected groups were compared to normal unstressed animal values and also to values in animals convulsed by oxygen unprotected by Li pre-treatment. 4. In rat brain abd blood significant (P less than 0-001) elevation of ammonia and glutamine and depression of gamma-amino butyric acid (brain only) and glutamate was noted following oxygen treatment in unprotected animals. Prior injection of Li 15 min before high pressure oxygen exposure delayed convulsions twice as long. Additionally if these animals were only exposed to oxygen for a period of time equal to that which would normally produce convulsions in unprotected animals, brain and blood ammonia and amino acids were maintained near to unstressed animal levels. Concomitantly, blood Li concentrations were considerably depressed below the values one would expect from the previously determined Li distribution time profile. 5. In rats exposed to high pressure oxygen 24 hr after Li treatment there was no protective action against high pressure oxygen convulsion, rather a potentiating effect for convulsion was seen. 6. These data present compelling evidence for the controlling effect of Li in rats, on rising blood ammonia concentration which occurs in high pressure oxygen exposure. The effect might well be due to the known chelating properties of Li with ammonia.", "PMID": 978569}
{"id": "pubmed23n0032_11781", "title": "Sulphate excretion by dogs following ingestion of ammonium sulphate or meat.", "content": "1. When normal conscious dogs were given small doses of (NH4)2SO4 by stomach tube, the rate of excretion of SO4 increased due to increase in plasma SO4 without change in creatinine clearance. After eating meat, increased SO4 excretion was due to increase in glomerular filtration rate without change in plasma SO4. 2. The calculated rate of tubular reabsorption of SO4 did not change significantly either after (NH4)2SO4 or after meat. 3. The rate of production of SO4 after meat was estimated. In 4 hr approximately one fifth of the S in meat was converted to inorganic SO4; in the same time approximately one third of the N in the meat was converted into urea. 4. Half of the SO4 in the doses of (NH4)2SO4 was absorbed from the gut; all of the NH was converted into urea. (NH4)2SO4 thus produced acidosis. 5. After (NH4)2SO4, increased excretion of NH4+ accompanied the increased excretion of SO4-but Na+, K+ in urine also increased.", "contents": "Sulphate excretion by dogs following ingestion of ammonium sulphate or meat. 1. When normal conscious dogs were given small doses of (NH4)2SO4 by stomach tube, the rate of excretion of SO4 increased due to increase in plasma SO4 without change in creatinine clearance. After eating meat, increased SO4 excretion was due to increase in glomerular filtration rate without change in plasma SO4. 2. The calculated rate of tubular reabsorption of SO4 did not change significantly either after (NH4)2SO4 or after meat. 3. The rate of production of SO4 after meat was estimated. In 4 hr approximately one fifth of the S in meat was converted to inorganic SO4; in the same time approximately one third of the N in the meat was converted into urea. 4. Half of the SO4 in the doses of (NH4)2SO4 was absorbed from the gut; all of the NH was converted into urea. (NH4)2SO4 thus produced acidosis. 5. After (NH4)2SO4, increased excretion of NH4+ accompanied the increased excretion of SO4-but Na+, K+ in urine also increased.", "PMID": 978570}
{"id": "pubmed23n0032_11782", "title": "Progressive enhancement in the secretory functions of the digestive system of the rat in the course of cold acclimation.", "content": "1. The secretory function of the exocrine pancreas and the stomach have been  studied in the course of cold acclimation of rats that had been fed at an ambient temperature of 1 degree C in a climatic room. 2. The secretory responses of pancreatic enzymes evoked by continuous infusion of pancreozymin (PZ, 2-5 mu./kg. hr) and a rapid single injection of PZ (1.7 mu./kg) reached a maximum in the group of rats fed at 1 degree C for 4  weeks, and fell to the control levels after 8 weeks. The increase in the flow of pancreatic juice evoked by single injection of PZ was maximal at 4 weeks and slightly decreased after 8 weeks. 3. The insulin (3-0 i.u./kg) evoked secretion of pancreatic enzymes gradually increased after cold exposure, reached a maximum at 4 weeks and fell to the control levels after 8 weeks. The flow of pancreatic juice after insulin injection was almost the same in every group throughout the course of cold exposure. 4. The ratio of amylase to the total amount of the protein in the pancreatic juice decreased abruptly, in contrast to an increase in the ratio of protease in the process of cold acclimation. The change in the ratio of enzyme activity in the pancreatic juice may reflect parallel changes in enzyme activity in the exocrine pancreas. 5. The gastric secretion in response to insulin and bile secretion in the group fed at 1 degree C for 7 weeks was significantly higher than that in the control group. 6. It was thus concluded that the secretory activities of digestive system were enhanced by prolonged cold exposure and then returned to control level, and that the activites of the pancreatic enzymes were altered in the process of cold acclimation in rats.", "contents": "Progressive enhancement in the secretory functions of the digestive system of the rat in the course of cold acclimation. 1. The secretory function of the exocrine pancreas and the stomach have been  studied in the course of cold acclimation of rats that had been fed at an ambient temperature of 1 degree C in a climatic room. 2. The secretory responses of pancreatic enzymes evoked by continuous infusion of pancreozymin (PZ, 2-5 mu./kg. hr) and a rapid single injection of PZ (1.7 mu./kg) reached a maximum in the group of rats fed at 1 degree C for 4  weeks, and fell to the control levels after 8 weeks. The increase in the flow of pancreatic juice evoked by single injection of PZ was maximal at 4 weeks and slightly decreased after 8 weeks. 3. The insulin (3-0 i.u./kg) evoked secretion of pancreatic enzymes gradually increased after cold exposure, reached a maximum at 4 weeks and fell to the control levels after 8 weeks. The flow of pancreatic juice after insulin injection was almost the same in every group throughout the course of cold exposure. 4. The ratio of amylase to the total amount of the protein in the pancreatic juice decreased abruptly, in contrast to an increase in the ratio of protease in the process of cold acclimation. The change in the ratio of enzyme activity in the pancreatic juice may reflect parallel changes in enzyme activity in the exocrine pancreas. 5. The gastric secretion in response to insulin and bile secretion in the group fed at 1 degree C for 7 weeks was significantly higher than that in the control group. 6. It was thus concluded that the secretory activities of digestive system were enhanced by prolonged cold exposure and then returned to control level, and that the activites of the pancreatic enzymes were altered in the process of cold acclimation in rats.", "PMID": 978571}
{"id": "pubmed23n0032_11783", "title": "Sodium-dependent influx of orthophosphate in mammalian non-myelinated nerve.", "content": "1. The rate of uptake of radiophosphate was measured in desheathed vagus nerves of rabbits mounted in an apparatus where the incubating solution flowed along the preparation. 2. Replacement of the Na of the Locke by either choline or Tris slowed the rate of uptake to about 10% of its value in Na; with K it was slowed to 20%; and with Li to 50%. 3. Measurements of the rate of uptake at different extracellular phosphate concentrations showed that in choline-Locke the influx of phosphate was proportional to the extracellular phosphate concentration, while in Locke the rate of inward flow showed a tendency to saturation with increasing phosphate concentrations. 4. Extracts of nerves after 45 min exposure to labelled solutions showed for different phosphate concentrations a slowing of the labelling of ATP, ADP and creatine-phosphate (CrP) and inorganic phosphate (Pi) when Locke was replaced by choline-Locke. 5. A similar slowing was found when, at 0-2 mM phosphate, the labelling of these compounds was measured at different times: application of choline-Locke reduced the rate of incorporation of 32P to about 15% of the rate in Locke. 6. In preparations that were loaded with radiophosphate and then washed with inactive solution, and the effluent fractionated, over 90% of the radioactivity was found in the inorganic phosphate fraction. 7. The efflux of 32P showed an initial exponential phase with a time constant of 20-30 min and a much slower one. The slow efflux had a rate constant of 0-0014 min-1 in 0-2 mM external phosphate. 8. Increasing the external phosphate concentration increased the efflux. 9. Prolonged incubation in choline-Locke reduced the efflux. 10. The influx of phosphate was calculated for different external phosphate concentrations using the rate of uptake of radiophosphate measured 45 min after the application of isotope and the corresponding efflux rate coefficient and the intracellular specific activities of the labelled compounds. 11. A correction was also introduced for diffusion, using the \"limited biophase model\". 12. The Na-sensitive influx, i.e. the difference between influx in Locke and influx in choline-Locke, showed saturation kinetics. 13. A Lineweaver-Burk plot for Na-sensitive uncorrected influxes gave a vmax of 16-7 mumole/kg wet wt. min and an apparent Km of 0-42 mM; for the corrected fluxes these values were 18-2 and 0-36. 14. It is concluded that a large part of phosphate influx and some of the phosphate efflux is mediated by a specific Na-dependent phosphate transport system. 15. This system seems to be present also in other types of nervous tissues and probably in many types of animal cells.", "contents": "Sodium-dependent influx of orthophosphate in mammalian non-myelinated nerve. 1. The rate of uptake of radiophosphate was measured in desheathed vagus nerves of rabbits mounted in an apparatus where the incubating solution flowed along the preparation. 2. Replacement of the Na of the Locke by either choline or Tris slowed the rate of uptake to about 10% of its value in Na; with K it was slowed to 20%; and with Li to 50%. 3. Measurements of the rate of uptake at different extracellular phosphate concentrations showed that in choline-Locke the influx of phosphate was proportional to the extracellular phosphate concentration, while in Locke the rate of inward flow showed a tendency to saturation with increasing phosphate concentrations. 4. Extracts of nerves after 45 min exposure to labelled solutions showed for different phosphate concentrations a slowing of the labelling of ATP, ADP and creatine-phosphate (CrP) and inorganic phosphate (Pi) when Locke was replaced by choline-Locke. 5. A similar slowing was found when, at 0-2 mM phosphate, the labelling of these compounds was measured at different times: application of choline-Locke reduced the rate of incorporation of 32P to about 15% of the rate in Locke. 6. In preparations that were loaded with radiophosphate and then washed with inactive solution, and the effluent fractionated, over 90% of the radioactivity was found in the inorganic phosphate fraction. 7. The efflux of 32P showed an initial exponential phase with a time constant of 20-30 min and a much slower one. The slow efflux had a rate constant of 0-0014 min-1 in 0-2 mM external phosphate. 8. Increasing the external phosphate concentration increased the efflux. 9. Prolonged incubation in choline-Locke reduced the efflux. 10. The influx of phosphate was calculated for different external phosphate concentrations using the rate of uptake of radiophosphate measured 45 min after the application of isotope and the corresponding efflux rate coefficient and the intracellular specific activities of the labelled compounds. 11. A correction was also introduced for diffusion, using the \"limited biophase model\". 12. The Na-sensitive influx, i.e. the difference between influx in Locke and influx in choline-Locke, showed saturation kinetics. 13. A Lineweaver-Burk plot for Na-sensitive uncorrected influxes gave a vmax of 16-7 mumole/kg wet wt. min and an apparent Km of 0-42 mM; for the corrected fluxes these values were 18-2 and 0-36. 14. It is concluded that a large part of phosphate influx and some of the phosphate efflux is mediated by a specific Na-dependent phosphate transport system. 15. This system seems to be present also in other types of nervous tissues and probably in many types of animal cells.", "PMID": 978572}
{"id": "pubmed23n0032_11784", "title": "The morphology of spinocervical tract neurones in the cat.", "content": "1. The morphology of physiologically identified spinocervical tract (SCT) neurones was studied using the intracellular injection of Procion dyes in anesthetized and decerebrate cats. 2. Extracellular recordings were made from SCT neurones at depths between 1000 and 2850 mum from the cord surface but neurones were only stained at depths between 1100 and 2400 mum. 3. The dendritic trees of stained SCT neurones were reconstructed in the transverse plane of the spinal cord. All SCT neurones had well developed dorsal dendrites but despite this it is not possible to consider the twenty-two SCT cells in out sample as consituting a morphologically homogenous population. 4. There was no correlation  between the form of the dendritic trees and the depth of SCT neurones in the dorsal horn as determined both from measurements from the dorsal grey-white border and the position of cells with respect to the border between Rexed's laminae II and III. 5. Six types of SCT neurones were identified on the basis of the form of their dendritic trees as viewed in the transverse plane: (1) radially symmetrical, (2) semicircular, (3) large elliptical, (4) bilobed, (5) triangular, (6) small elliptical. Each of these types was found only in a certain region across the dorsal horn although any one region could contain more than one type. 6. Spinocervical tract neurones with small elliptical dendritic trees always had receptive fields encompassing part of the hip or thigh and were unique in being located in the lateral portions of the horn. 7. There was no correlation between the morphology of SCT neurones and their excitatory cutaneous inputs, receptive field size, axonal conduction velocity or depth in the dorsal horn.", "contents": "The morphology of spinocervical tract neurones in the cat. 1. The morphology of physiologically identified spinocervical tract (SCT) neurones was studied using the intracellular injection of Procion dyes in anesthetized and decerebrate cats. 2. Extracellular recordings were made from SCT neurones at depths between 1000 and 2850 mum from the cord surface but neurones were only stained at depths between 1100 and 2400 mum. 3. The dendritic trees of stained SCT neurones were reconstructed in the transverse plane of the spinal cord. All SCT neurones had well developed dorsal dendrites but despite this it is not possible to consider the twenty-two SCT cells in out sample as consituting a morphologically homogenous population. 4. There was no correlation  between the form of the dendritic trees and the depth of SCT neurones in the dorsal horn as determined both from measurements from the dorsal grey-white border and the position of cells with respect to the border between Rexed's laminae II and III. 5. Six types of SCT neurones were identified on the basis of the form of their dendritic trees as viewed in the transverse plane: (1) radially symmetrical, (2) semicircular, (3) large elliptical, (4) bilobed, (5) triangular, (6) small elliptical. Each of these types was found only in a certain region across the dorsal horn although any one region could contain more than one type. 6. Spinocervical tract neurones with small elliptical dendritic trees always had receptive fields encompassing part of the hip or thigh and were unique in being located in the lateral portions of the horn. 7. There was no correlation between the morphology of SCT neurones and their excitatory cutaneous inputs, receptive field size, axonal conduction velocity or depth in the dorsal horn.", "PMID": 978573}
{"id": "pubmed23n0032_11785", "title": "The secretion of citrate into milk.", "content": "1. The time course of changes in specific activities of citrate, lactose and fatty acids in milk during frequent milking, following the I.V. administration of labelled glucose, acetate and chylomicrons in goats has been studied. Peak specific activities of lactose and citrate in milk were reached at 2-3 hr, while peak specific activites of fatty acids were reached at 5-7 hr. 2. Following short I.A. infusions of 24Na, 36Cl, and 42K, peak specific activities in milk were reached in 1 hr or less. 3. The mammary epithelium of lactating goats was found to be virtually impermeable to labelled citrate in both directions. 4. Labelled citrate had an apparent volume of distribution in lactating guinea-pigs mammary slices in vitro similar to that of extracellular space markers. 5. Treatment of goats with large doses of oxytocin markedly increased the permeability of the secretory epithelium to labelled citrate. 6. In the goat mammary gland, citrate, protein and calcium failed to enter milk which had been diluted with isosmotic lactose by intraductal injection, whereas Na, K and Cl did enter, thus tending to restore the concentrations of these ions to normal. 7. It is suggested that citrate, which is formed within the sucretory cell, enters milk not by passage across the apical cell membrane but, in common with lactose and milk protein, by exocytosis of Golgi vesicles. It appears that citrate is held at high concentrations in milk by virtue of the impermeability of the mammary epithelium to the forms in which it occurs in milk.", "contents": "The secretion of citrate into milk. 1. The time course of changes in specific activities of citrate, lactose and fatty acids in milk during frequent milking, following the I.V. administration of labelled glucose, acetate and chylomicrons in goats has been studied. Peak specific activities of lactose and citrate in milk were reached at 2-3 hr, while peak specific activites of fatty acids were reached at 5-7 hr. 2. Following short I.A. infusions of 24Na, 36Cl, and 42K, peak specific activities in milk were reached in 1 hr or less. 3. The mammary epithelium of lactating goats was found to be virtually impermeable to labelled citrate in both directions. 4. Labelled citrate had an apparent volume of distribution in lactating guinea-pigs mammary slices in vitro similar to that of extracellular space markers. 5. Treatment of goats with large doses of oxytocin markedly increased the permeability of the secretory epithelium to labelled citrate. 6. In the goat mammary gland, citrate, protein and calcium failed to enter milk which had been diluted with isosmotic lactose by intraductal injection, whereas Na, K and Cl did enter, thus tending to restore the concentrations of these ions to normal. 7. It is suggested that citrate, which is formed within the sucretory cell, enters milk not by passage across the apical cell membrane but, in common with lactose and milk protein, by exocytosis of Golgi vesicles. It appears that citrate is held at high concentrations in milk by virtue of the impermeability of the mammary epithelium to the forms in which it occurs in milk.", "PMID": 978574}
{"id": "pubmed23n0032_11786", "title": "Release of noradrenaline from the cat spleen by nerve stimulation and potassium.", "content": "1. Release of noradrenaline from the perfused cat spleen, or from isolated spleen slices, in response to prolonged nerve stimulation or maintained depolarization by potassium was measured. 2. Prolonged stimulation of the splenic nerves at 2, 10 and 30 Hz for 10 min evoked release, which was maximum during the first 2 min, and then declined during the remaining period of stimulation. When noradrenaline release was induced by high potassium from the perfused spleen or from isolated slices, it followed a similar time course to nerve stimulation. Similar results were obtained from phenoxybenzamine-treated spleens, using both modes of stimulation. 3. Stimulation of the splenic nerves in calcium-free Krebs solution did not release noradrenaline. If calcium was introduced at a later stage during stimulation, the release was markedly diminished. In phenoxybenzamine- or phentolamine-treated spleens, stimulation of the nerves in the presence of calcium evoked a secretory response which was comparable to the one produced by introduction of calcium after a few minutes of nerve stimulation. 4. Simultaneous application of calcium plus high potassium always produced a much greater secretion of noradrenaline than application of calcium after a few minutes of potassium depolarization. Release of noradrenaline by potassium from phenoxybenzamine-treated spleens was also much greater if calcium and potassium were added simultaneously than addition of calcium after a few minutes of potassium depolarization. 5. In the presence of maintained depolarization by potasssium, tyramine was effective in causing release of noradrenaline.", "contents": "Release of noradrenaline from the cat spleen by nerve stimulation and potassium. 1. Release of noradrenaline from the perfused cat spleen, or from isolated spleen slices, in response to prolonged nerve stimulation or maintained depolarization by potassium was measured. 2. Prolonged stimulation of the splenic nerves at 2, 10 and 30 Hz for 10 min evoked release, which was maximum during the first 2 min, and then declined during the remaining period of stimulation. When noradrenaline release was induced by high potassium from the perfused spleen or from isolated slices, it followed a similar time course to nerve stimulation. Similar results were obtained from phenoxybenzamine-treated spleens, using both modes of stimulation. 3. Stimulation of the splenic nerves in calcium-free Krebs solution did not release noradrenaline. If calcium was introduced at a later stage during stimulation, the release was markedly diminished. In phenoxybenzamine- or phentolamine-treated spleens, stimulation of the nerves in the presence of calcium evoked a secretory response which was comparable to the one produced by introduction of calcium after a few minutes of nerve stimulation. 4. Simultaneous application of calcium plus high potassium always produced a much greater secretion of noradrenaline than application of calcium after a few minutes of potassium depolarization. Release of noradrenaline by potassium from phenoxybenzamine-treated spleens was also much greater if calcium and potassium were added simultaneously than addition of calcium after a few minutes of potassium depolarization. 5. In the presence of maintained depolarization by potasssium, tyramine was effective in causing release of noradrenaline.", "PMID": 978575}
{"id": "pubmed23n0032_11787", "title": "The secretory characteristics of dehydrocholate in the dog: comparison with the natural bile salts.", "content": "1. During dehydrocholate administration in the taurine replete dog, the maximum excretory rate of total bile salt (almost entirely dehydrocholate derivative, mostly conjugated) was 3-84 +/- 0-53 (S.D.) mumole/min. kg body wt. (eleven experiments). This was much less than the excretory maximum previously obtained for taurocholate (8-64 +/- 1-31 (S.D.) mumole/min. kg total cholate, mostly conjugated). 2. The superimposition of taurocholate infusion did not cause any significant change in the 'dehydrocholate' maximum but taurocholate itself was excreted into bile at no more than about half its normal maximum. When taurocholate maximum excretion was established first, it was reduced by dehydrocholate administration. In both types of experiment the joint bile salt excretory maximum was of the same order as that of taurocholate alone, provided taurocholate made up at least 40-50% of the total bile salt. 3. When taurocholate administration was stopped, the maximum excretory rate of 'dehydrocholate' rose to values up to 63% above the initially determined excretory maximum; the enhanced 'dehydrocholate' excretory maximum, when calculated for optimal conditions, approached that of actively conjugated vholate, even though the effective 'dehydrocholate' concentration in bile was ten to twenty times the critical micellar concentration of taurocholate. This suggests that the effective bile salt concentration in bile is not an important determinant of the secretory performance of a bile salt. 4. To explain findings (2) and (3) it is necessary to postulate that taurocholate has both a facilitatory and an inhibitory action on 'dehydrocholate' excretion. The facilitatory action, which persists after taurocholate has left the animal, may consist either of an increase in the maximum rate at which modification of dehydrocholate takes place within the liver cell, or an increase in the number of functioning 'carriers' for 'dehydrocholate' transfer. The data suggest that the inhibitory effect is due to the competitive interaction that also appears to exist between the two bile salts. 5. The increase in bile flow rate per unit increase in 'dehydrocholate' excretion (15 ml./m-mole) was about twice that obtained for taurocholate. There was no significant formation of micellar aggregates during 'dehydrocholate' excretion, as judged from the total electrolyte concentration of bile and its osmalality. 6. During the excretion of 'dehydrocholate'-taurocholate mixtures (approximately 1:1) at submaximal rates the associated bile flow rate was not less than the sum of the separate components, thus suggesting that 'dehydrocholate' was not being incorporated in taurocholate mixed micelles.", "contents": "The secretory characteristics of dehydrocholate in the dog: comparison with the natural bile salts. 1. During dehydrocholate administration in the taurine replete dog, the maximum excretory rate of total bile salt (almost entirely dehydrocholate derivative, mostly conjugated) was 3-84 +/- 0-53 (S.D.) mumole/min. kg body wt. (eleven experiments). This was much less than the excretory maximum previously obtained for taurocholate (8-64 +/- 1-31 (S.D.) mumole/min. kg total cholate, mostly conjugated). 2. The superimposition of taurocholate infusion did not cause any significant change in the 'dehydrocholate' maximum but taurocholate itself was excreted into bile at no more than about half its normal maximum. When taurocholate maximum excretion was established first, it was reduced by dehydrocholate administration. In both types of experiment the joint bile salt excretory maximum was of the same order as that of taurocholate alone, provided taurocholate made up at least 40-50% of the total bile salt. 3. When taurocholate administration was stopped, the maximum excretory rate of 'dehydrocholate' rose to values up to 63% above the initially determined excretory maximum; the enhanced 'dehydrocholate' excretory maximum, when calculated for optimal conditions, approached that of actively conjugated vholate, even though the effective 'dehydrocholate' concentration in bile was ten to twenty times the critical micellar concentration of taurocholate. This suggests that the effective bile salt concentration in bile is not an important determinant of the secretory performance of a bile salt. 4. To explain findings (2) and (3) it is necessary to postulate that taurocholate has both a facilitatory and an inhibitory action on 'dehydrocholate' excretion. The facilitatory action, which persists after taurocholate has left the animal, may consist either of an increase in the maximum rate at which modification of dehydrocholate takes place within the liver cell, or an increase in the number of functioning 'carriers' for 'dehydrocholate' transfer. The data suggest that the inhibitory effect is due to the competitive interaction that also appears to exist between the two bile salts. 5. The increase in bile flow rate per unit increase in 'dehydrocholate' excretion (15 ml./m-mole) was about twice that obtained for taurocholate. There was no significant formation of micellar aggregates during 'dehydrocholate' excretion, as judged from the total electrolyte concentration of bile and its osmalality. 6. During the excretion of 'dehydrocholate'-taurocholate mixtures (approximately 1:1) at submaximal rates the associated bile flow rate was not less than the sum of the separate components, thus suggesting that 'dehydrocholate' was not being incorporated in taurocholate mixed micelles.", "PMID": 978576}
{"id": "pubmed23n0032_11788", "title": "The direct effect on pulmonary stretch receptor discharge produced by changing lung carbon dioxide concentration in dogs on cardiopulmonary bypass and its action on breathing.", "content": "1. Single fibre pulmonary stretch receptor discharge was recorded in dogs on cardiopulmonary bypass. 2. Inhalation of CO2 depressed pulmonary stretch receptor discharge despite the absence of changes in arterial PCO2. This effect was particularly marked with airway CO2 levels below 5%. 3. Changing arterial PCO2, without changing airway CO2, had only small and insignificant effects on pulmonary stretch receptor discharge. 4. The effect of changes in airway CO2 on pulmonary stretch receptor discharge was rapid and correlated well in time with the reflex tachypnoea produced when CO2 was inhaled in conditions of cardiopulmonary bypass. 5. Stimulation of the central end of the cut vagus nerve was triggered from simultaneously recorded action potentials from a single pulmonary stretch receptor. 6. In these conditions, the reflex response to CO2 could be simulated provided that the pulmonary stretch receptor had an end-expiratory discharge. 7. It is suggested that the vagally mediated tachypnoeic response to changes in airway CO2 seen in conditions of cardiopulmonary bypass is due to the effect of CO2 on the end-expiratory discharge of pulmonary stretch receptors.", "contents": "The direct effect on pulmonary stretch receptor discharge produced by changing lung carbon dioxide concentration in dogs on cardiopulmonary bypass and its action on breathing. 1. Single fibre pulmonary stretch receptor discharge was recorded in dogs on cardiopulmonary bypass. 2. Inhalation of CO2 depressed pulmonary stretch receptor discharge despite the absence of changes in arterial PCO2. This effect was particularly marked with airway CO2 levels below 5%. 3. Changing arterial PCO2, without changing airway CO2, had only small and insignificant effects on pulmonary stretch receptor discharge. 4. The effect of changes in airway CO2 on pulmonary stretch receptor discharge was rapid and correlated well in time with the reflex tachypnoea produced when CO2 was inhaled in conditions of cardiopulmonary bypass. 5. Stimulation of the central end of the cut vagus nerve was triggered from simultaneously recorded action potentials from a single pulmonary stretch receptor. 6. In these conditions, the reflex response to CO2 could be simulated provided that the pulmonary stretch receptor had an end-expiratory discharge. 7. It is suggested that the vagally mediated tachypnoeic response to changes in airway CO2 seen in conditions of cardiopulmonary bypass is due to the effect of CO2 on the end-expiratory discharge of pulmonary stretch receptors.", "PMID": 978577}
{"id": "pubmed23n0032_11789", "title": "Respiratory and thermoregulatory responses of rabbits breathing carbon dioxide during heat exposure.", "content": "1. Rabbits were clipped and exposed in turn to three environmental conditions: control (C), cold exposure (CE) and water deprivation (WD). Following each type of treatment, the rabbits were exposed to an ambient temperature (Ta) of 35 degrees C for 1 hr. Throughout this period they breathed either normal atmospheric air or 6% CO2 in air. 2. During heat exposure, measurements were made of the respiratory responses and of the O2 consumption (Vo2) of the rabbits. Rectal temperature (Tre) was measured immediately before and again immediately after heat exposure. 3. When subjected to cold exposure or water deprivation the rabbits showed an initial decrease in respiratory frequency (RF) and an initial increase in VT when compared with controls. There was no difference in VE. Rabbits breathing 6% CO2 showed an increase in VT and VE and a decrease in RF when compared with rabbits breathing atmospheric air. In all cases a change in VT or RF was associated with a reciprocal change in the other parameter. 4. The respiratory responses to breathing 6% CO2 were essentially similar in treated and control rabbits, from which it is concluded that neither cold exposure nor water deprivation alter the sensitivity of the medullary respiratory centre to the respiratory drive from the central chemosensors. 5. The increase in Tre during heat exposure was significantly less in rabbits breathing 6% CO2 than in rabbits breathing atmospheric air. However, there was no significant over-all difference in VO2 between rabbits breathing CO2 and those breathing air. From this it is concluded that increased ventilation induced by CO2 causes a greater dissipation of heat than does thermally-induced panting. 6. It is concluded that VT is controlled by the level of blood PCO2 whereas RF is controlled by thermoregulatory requirements. It is further concluded that the reciprocal relationship between VT and RF is regulated in such a way as to maintain VE at the appropriate level for effecting gaseous exchange and evaporative heat loss.", "contents": "Respiratory and thermoregulatory responses of rabbits breathing carbon dioxide during heat exposure. 1. Rabbits were clipped and exposed in turn to three environmental conditions: control (C), cold exposure (CE) and water deprivation (WD). Following each type of treatment, the rabbits were exposed to an ambient temperature (Ta) of 35 degrees C for 1 hr. Throughout this period they breathed either normal atmospheric air or 6% CO2 in air. 2. During heat exposure, measurements were made of the respiratory responses and of the O2 consumption (Vo2) of the rabbits. Rectal temperature (Tre) was measured immediately before and again immediately after heat exposure. 3. When subjected to cold exposure or water deprivation the rabbits showed an initial decrease in respiratory frequency (RF) and an initial increase in VT when compared with controls. There was no difference in VE. Rabbits breathing 6% CO2 showed an increase in VT and VE and a decrease in RF when compared with rabbits breathing atmospheric air. In all cases a change in VT or RF was associated with a reciprocal change in the other parameter. 4. The respiratory responses to breathing 6% CO2 were essentially similar in treated and control rabbits, from which it is concluded that neither cold exposure nor water deprivation alter the sensitivity of the medullary respiratory centre to the respiratory drive from the central chemosensors. 5. The increase in Tre during heat exposure was significantly less in rabbits breathing 6% CO2 than in rabbits breathing atmospheric air. However, there was no significant over-all difference in VO2 between rabbits breathing CO2 and those breathing air. From this it is concluded that increased ventilation induced by CO2 causes a greater dissipation of heat than does thermally-induced panting. 6. It is concluded that VT is controlled by the level of blood PCO2 whereas RF is controlled by thermoregulatory requirements. It is further concluded that the reciprocal relationship between VT and RF is regulated in such a way as to maintain VE at the appropriate level for effecting gaseous exchange and evaporative heat loss.", "PMID": 978578}
{"id": "pubmed23n0032_11790", "title": "Polyneuronal innervation of skeletal muscle in new-born rats and its elimination during maturation.", "content": "1. The events taking place during the elimination of polyneuronal innervation in the soleus muscle of new-born rats have been studied using a combination of electrophysiological and anatomical techniques. 2. Each immature muscle fibre is supplied by two or more motor axons which converge on to a single end-plate. There was no sign of electrical coupling between muscle fibres receiving multiple synaptic inputs. By the end of the second week after birth virtually all muscle fibres are innervated by only a single motor axon. 3. The average tension produced by individual motor units, measured in terms of the percentage of the total muscle twitch tension, declined dramatically during the first 2 weeks after birth. During this period there was no significant change in the number of motor neurones innervating the soleus muscle. Thus, the disappearance of polyneuronal innervation reflects a decrease in the number of peripheral synapses made by each motor neurone. 4. The decline in motor unit size was delayed, but not ultimately prevented, by the early surgical removal of all but a few motor axons to the soleus muscle. This procedure also caused a delay in the removal of polyneuronal innervation involving the remaining motor units. 5. Following a crush of the soleus nerve in neonatal animals, regenerating axons usually returned to the original end-plates. Polyneuronal innervation was extensive at early stages of re-innervation and it disappeared during the second week after birth just as in normal muscles. 6. Cross-innervation of neonatal muscles by an implanted foreign nerve caused a rapid disappearance of cholinesterase at denervated original end-plates and in most fibres prevented re-innervation by the original nerve. In the small proportion of fibres that did become innervated through both the foreign and original nerves the end-plates were more than 1 mm apart, and both foreign and original nerve end-plates could persist indefinitely. 7. Many cross-innervated fibres received multiple inputs through the foreign nerve. Some foreign end-plates were separated by distances ranging up to 1 mm. Polyneuronal innervation through the foreign nerve was completely eliminated during maturation but over a slightly longer period than in normal muscles. Apparently the elimination process can act over a distance up to but not much more than 1 mm. 8. These observations suggest that there are several factors influencing the elimination of redundant inputs in immature muscles. Individual motor neurones appear to have an inherent tendency to withdraw the majority of their original complement of peripheral terminals. The determination of which particular synapses are to survive, however, seems to be made in the periphery by a selection among all the synapses that innervate a limited region of each muscle fibre. There may be a competitive interaction among synapses in which those belonging to smaller motor units are less likely to be eliminated, thereby leading to a relatively uniform size of the motor units in the soleus.", "contents": "Polyneuronal innervation of skeletal muscle in new-born rats and its elimination during maturation. 1. The events taking place during the elimination of polyneuronal innervation in the soleus muscle of new-born rats have been studied using a combination of electrophysiological and anatomical techniques. 2. Each immature muscle fibre is supplied by two or more motor axons which converge on to a single end-plate. There was no sign of electrical coupling between muscle fibres receiving multiple synaptic inputs. By the end of the second week after birth virtually all muscle fibres are innervated by only a single motor axon. 3. The average tension produced by individual motor units, measured in terms of the percentage of the total muscle twitch tension, declined dramatically during the first 2 weeks after birth. During this period there was no significant change in the number of motor neurones innervating the soleus muscle. Thus, the disappearance of polyneuronal innervation reflects a decrease in the number of peripheral synapses made by each motor neurone. 4. The decline in motor unit size was delayed, but not ultimately prevented, by the early surgical removal of all but a few motor axons to the soleus muscle. This procedure also caused a delay in the removal of polyneuronal innervation involving the remaining motor units. 5. Following a crush of the soleus nerve in neonatal animals, regenerating axons usually returned to the original end-plates. Polyneuronal innervation was extensive at early stages of re-innervation and it disappeared during the second week after birth just as in normal muscles. 6. Cross-innervation of neonatal muscles by an implanted foreign nerve caused a rapid disappearance of cholinesterase at denervated original end-plates and in most fibres prevented re-innervation by the original nerve. In the small proportion of fibres that did become innervated through both the foreign and original nerves the end-plates were more than 1 mm apart, and both foreign and original nerve end-plates could persist indefinitely. 7. Many cross-innervated fibres received multiple inputs through the foreign nerve. Some foreign end-plates were separated by distances ranging up to 1 mm. Polyneuronal innervation through the foreign nerve was completely eliminated during maturation but over a slightly longer period than in normal muscles. Apparently the elimination process can act over a distance up to but not much more than 1 mm. 8. These observations suggest that there are several factors influencing the elimination of redundant inputs in immature muscles. Individual motor neurones appear to have an inherent tendency to withdraw the majority of their original complement of peripheral terminals. The determination of which particular synapses are to survive, however, seems to be made in the periphery by a selection among all the synapses that innervate a limited region of each muscle fibre. There may be a competitive interaction among synapses in which those belonging to smaller motor units are less likely to be eliminated, thereby leading to a relatively uniform size of the motor units in the soleus.", "PMID": 978579}
{"id": "pubmed23n0032_11791", "title": "The conditions required for the maintenance of binocularity in the kitten's visual cortex.", "content": "1. In young kittens, cortical neurones, which are usually binocularly driven, have their binocularity reduced if one eye is covered, or if the eyes are made strabismic or alternately occluded. Some of the factors causing these changes were analysed. 2. If the contrast of one retinal image is abolished with no difference in mean illumination, the input from that eye is virtually lost. 3. If one eye merely has its mean retinal illumination attenuated, that eye does not specifically lose its influence in the cortex, although there is a reduction in the proportion of binocular units. This change might partly be due to a difference in the timing of signals from the two eyes but is more likely to be caused by a difference in the strength of the discharges. 4. There is little change in binocularity if one image is dimmed but contrast is absent from both. 5. If contours of very different orientation fall simultaneously on corresponding retinal regions, binocularity breaks down, as in the case of strabismus or when different patterns are presented to the two eyes. But as long as the patterns on corresponding retinal points have similar orientation, even if the visual axes are misaligned, binocularity can be maintained. 6. If the eyes are not stimulated simultaneously, binocularity is reduced, even if the contours falling on the two retinae (at different times) are identical. 7. Roughly simultaneous stimulation, with roughly congruent patterns on the two receptive fields, are needed for the upkeep of binocular connexions on to cortical cells.", "contents": "The conditions required for the maintenance of binocularity in the kitten's visual cortex. 1. In young kittens, cortical neurones, which are usually binocularly driven, have their binocularity reduced if one eye is covered, or if the eyes are made strabismic or alternately occluded. Some of the factors causing these changes were analysed. 2. If the contrast of one retinal image is abolished with no difference in mean illumination, the input from that eye is virtually lost. 3. If one eye merely has its mean retinal illumination attenuated, that eye does not specifically lose its influence in the cortex, although there is a reduction in the proportion of binocular units. This change might partly be due to a difference in the timing of signals from the two eyes but is more likely to be caused by a difference in the strength of the discharges. 4. There is little change in binocularity if one image is dimmed but contrast is absent from both. 5. If contours of very different orientation fall simultaneously on corresponding retinal regions, binocularity breaks down, as in the case of strabismus or when different patterns are presented to the two eyes. But as long as the patterns on corresponding retinal points have similar orientation, even if the visual axes are misaligned, binocularity can be maintained. 6. If the eyes are not stimulated simultaneously, binocularity is reduced, even if the contours falling on the two retinae (at different times) are identical. 7. Roughly simultaneous stimulation, with roughly congruent patterns on the two receptive fields, are needed for the upkeep of binocular connexions on to cortical cells.", "PMID": 978580}
{"id": "pubmed23n0032_11792", "title": "The possible mechanism for the 'fade' in acid gastric secretion during continuous infusions of pentagastrin.", "content": "1. The 'fade' phenomenon seen in acid gastric secretion in response to a continuous intravenous infusion of pentagastrin has been studied in anaesthetized cats. Whereas acid secretion to a continuous infusion of histamine steadily increased for 3 hr, that to pentagastrin infusion rose steeply to a maximum at 45 min and then decreased gradually to reach about 60% of maximum after 2 hr. 2. Pre-treatment of the cats with the 5-hydroxytryptamine (5-HT) blocking drugs, methysergide and cyproheptadine or with the 5-hydroxytryptamine-depleting drug reserpine completely altered the pattern of secretion to pentagastrin such that the response was similar to that seen during an infusion of histamine. These drugs did not affect secretion stimulated by histamine. 3. It is suggested from these results that pentagastrin releases 5-hydroxytryptamine which at first potentiates and then inhibits the resulting stimulated secretion.", "contents": "The possible mechanism for the 'fade' in acid gastric secretion during continuous infusions of pentagastrin. 1. The 'fade' phenomenon seen in acid gastric secretion in response to a continuous intravenous infusion of pentagastrin has been studied in anaesthetized cats. Whereas acid secretion to a continuous infusion of histamine steadily increased for 3 hr, that to pentagastrin infusion rose steeply to a maximum at 45 min and then decreased gradually to reach about 60% of maximum after 2 hr. 2. Pre-treatment of the cats with the 5-hydroxytryptamine (5-HT) blocking drugs, methysergide and cyproheptadine or with the 5-hydroxytryptamine-depleting drug reserpine completely altered the pattern of secretion to pentagastrin such that the response was similar to that seen during an infusion of histamine. These drugs did not affect secretion stimulated by histamine. 3. It is suggested from these results that pentagastrin releases 5-hydroxytryptamine which at first potentiates and then inhibits the resulting stimulated secretion.", "PMID": 978581}
{"id": "pubmed23n0032_11793", "title": "Competitive and non-competitive re-innervation of mammalian sympathetic neurones by native and foreign fibres.", "content": "The ability of native (sympathetic preganglionic) and foreign (vagal) nerve fibres to re-innervate neurones of the guinea-pig superior cervical ganglion, either alone or in competition with each other, has been studied by means of intracellular recording and electron microscopy. 1. Native fibres make synaptic contacts with nearly all ganglion cells within one month of cervical trunk section; within 6 months the degree of innervation, judged by measurement of excitatory post-synaptic potential (e.p.s.p.) amplitude and electron microscopical synapse counts, approaches normal. However, even after 15 months innervation was weaker than in normal control ganglia. 2. Vagal fibres are less successful during re-innervation. Although a similar number of foreign fibres grown into denervated ganglia and make contact with nearly all ganglion cells within a month, after 6-12 months e.p.s.p. amplitudes in response to foreign nerve stimulation remain relatively small, and counts of synapses are only about 60% as great as in ganglia re-innervated with the native nerve. 3. When both native and foreign fibres are allowed to re-innervate ganglion cells simultaneously, about half the neurones in the ganglion receive synapses from both sources after 1 month. The proportion of dually invervated cells remains roughly constant for at least 14 months. Neither set of preganglionic fibres dominates or displaces the other, although neurones generally are re-innervated more effectively by native than foreign fibres, as is true during non-competitive re-innervation. 4. Thus during re-innervation of mammalian sympathetic neurones native fibres are preferred to foreign ones only in the sense that roughly the same number of native fibres form many more synapses on ganglion cells than do vagal axons. A foreign synapse, once formed, is as stable as a native one, and shows no tendency to be replaced by native terminals. These findings are discussed in relation to other evidence which has suggested specificity and selectivity during re-innervation of mammalian autonomic neurones.", "contents": "Competitive and non-competitive re-innervation of mammalian sympathetic neurones by native and foreign fibres. The ability of native (sympathetic preganglionic) and foreign (vagal) nerve fibres to re-innervate neurones of the guinea-pig superior cervical ganglion, either alone or in competition with each other, has been studied by means of intracellular recording and electron microscopy. 1. Native fibres make synaptic contacts with nearly all ganglion cells within one month of cervical trunk section; within 6 months the degree of innervation, judged by measurement of excitatory post-synaptic potential (e.p.s.p.) amplitude and electron microscopical synapse counts, approaches normal. However, even after 15 months innervation was weaker than in normal control ganglia. 2. Vagal fibres are less successful during re-innervation. Although a similar number of foreign fibres grown into denervated ganglia and make contact with nearly all ganglion cells within a month, after 6-12 months e.p.s.p. amplitudes in response to foreign nerve stimulation remain relatively small, and counts of synapses are only about 60% as great as in ganglia re-innervated with the native nerve. 3. When both native and foreign fibres are allowed to re-innervate ganglion cells simultaneously, about half the neurones in the ganglion receive synapses from both sources after 1 month. The proportion of dually invervated cells remains roughly constant for at least 14 months. Neither set of preganglionic fibres dominates or displaces the other, although neurones generally are re-innervated more effectively by native than foreign fibres, as is true during non-competitive re-innervation. 4. Thus during re-innervation of mammalian sympathetic neurones native fibres are preferred to foreign ones only in the sense that roughly the same number of native fibres form many more synapses on ganglion cells than do vagal axons. A foreign synapse, once formed, is as stable as a native one, and shows no tendency to be replaced by native terminals. These findings are discussed in relation to other evidence which has suggested specificity and selectivity during re-innervation of mammalian autonomic neurones.", "PMID": 978582}
{"id": "pubmed23n0032_11794", "title": "A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves.", "content": "1. A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin. 2. The radiochemical purity of the labelled toxin has been directly determined, rather than being based on indirect biochemical means, as in previous experiments with Wilzbach-labelled STX and TTX. 3. The specific activity of the labelled toxin, 66 d..m.f-mole-1, corresponds with one tritium atom per molecule STX, an improvement of about 300-fold over other means of labelling TTX and STX. 4. The binding of this toxin to rabbit, lobster and garfish olfactory nerve fibres has been re-examined. 5. The density of sodium channels calculated on the basis of the binding of the toxin is about four to six-times the values previously reported.", "contents": "A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves. 1. A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin. 2. The radiochemical purity of the labelled toxin has been directly determined, rather than being based on indirect biochemical means, as in previous experiments with Wilzbach-labelled STX and TTX. 3. The specific activity of the labelled toxin, 66 d..m.f-mole-1, corresponds with one tritium atom per molecule STX, an improvement of about 300-fold over other means of labelling TTX and STX. 4. The binding of this toxin to rabbit, lobster and garfish olfactory nerve fibres has been re-examined. 5. The density of sodium channels calculated on the basis of the binding of the toxin is about four to six-times the values previously reported.", "PMID": 978583}
{"id": "pubmed23n0032_11795", "title": "Non-random distribution of cell types in the preoptic and anterior hypothalamic areas.", "content": "1. Single units were recorded extracellularly from the preoptic and anterior hypothalamic areas of male and female rats anaesthetized with urethane. 2. The effect of single shock stimulation of both the mediobasal hypothalamus and corticomedial amygdala upon unit activity was assessed for all cells. Neurones were designated either antidromically invaded, orthodromically excited or inhibited, or non-responsive. 3. The distribution of responses among recorded cells was analysed for two groups of neurones. In one group the intercell distance was less than 100 mum and in the second group the distance was more than 1 mm. 4. For widely separated cells the connexions with the corticomedial amygdala and mediobasal hypothalamus were distributed at random. 5. In the groups of cells clustered closely together a marked structuring of connexions was apparant. In particular, there was a significant correlation between orthodromic responses from the amygdala (P less than 0-05) and cells antidromically activated and orthodromically excited by stimulation of the mediobasal hypothalamus were frequently adjacent to one another (P less than 0-02. 6. A complimentary analysis demonstrated that cells in the ventral half of the recording zone received significantly more inhibitory inputs from both the mediobasal hypothalamus (P less than 0-001) and the corticomedial amygdala (P less than 0-02) than those situated more dorsally. 7. The data provide evidence for structuring and orderliness in hypothalamic connexions that is often not apparent from descriptions of electrophysiological experiments.", "contents": "Non-random distribution of cell types in the preoptic and anterior hypothalamic areas. 1. Single units were recorded extracellularly from the preoptic and anterior hypothalamic areas of male and female rats anaesthetized with urethane. 2. The effect of single shock stimulation of both the mediobasal hypothalamus and corticomedial amygdala upon unit activity was assessed for all cells. Neurones were designated either antidromically invaded, orthodromically excited or inhibited, or non-responsive. 3. The distribution of responses among recorded cells was analysed for two groups of neurones. In one group the intercell distance was less than 100 mum and in the second group the distance was more than 1 mm. 4. For widely separated cells the connexions with the corticomedial amygdala and mediobasal hypothalamus were distributed at random. 5. In the groups of cells clustered closely together a marked structuring of connexions was apparant. In particular, there was a significant correlation between orthodromic responses from the amygdala (P less than 0-05) and cells antidromically activated and orthodromically excited by stimulation of the mediobasal hypothalamus were frequently adjacent to one another (P less than 0-02. 6. A complimentary analysis demonstrated that cells in the ventral half of the recording zone received significantly more inhibitory inputs from both the mediobasal hypothalamus (P less than 0-001) and the corticomedial amygdala (P less than 0-02) than those situated more dorsally. 7. The data provide evidence for structuring and orderliness in hypothalamic connexions that is often not apparent from descriptions of electrophysiological experiments.", "PMID": 978584}
{"id": "pubmed23n0032_11796", "title": "The potentiating influences of insulin on pancreozymin-induced hyperpolarization and amylase release in the pancreatic acinar cell.", "content": "1. Insulin (1 mu./ml.) potentiated the release of amylase from isolated pancreas of rats perfused with erythrocyte-containing medium and stimulated by 0-5 m-u. pancreozymin (Pz)/ml., whereas the same concentration of insulin failed to potentiate the response evoked by or 200 m-u. PZ/ml. 2. Intracellular measurement of membrane potentials from the acinar cells of the same preparations showed that insulin (1 mu./ml.) simultaneously potentiates the hyperpolarization and amylase release in response to 0-5 m-u. PZ/ml. 3. These effects of insulin on the PZ-induced responses were inhibited by ouabain (3 X 10(-5) M). 4. The results suggest that insulin, in the concentration studied, has a potentiating action on the activity of an electrogenic Na pump, which maintains the hyperpolarization and amylase release during continuous stimulation with Pz. 5. Insulin in also potentiated the Pz-induced amylase release in the rat pancreata in situ after vagotomy and ligature of the pyloric region.", "contents": "The potentiating influences of insulin on pancreozymin-induced hyperpolarization and amylase release in the pancreatic acinar cell. 1. Insulin (1 mu./ml.) potentiated the release of amylase from isolated pancreas of rats perfused with erythrocyte-containing medium and stimulated by 0-5 m-u. pancreozymin (Pz)/ml., whereas the same concentration of insulin failed to potentiate the response evoked by or 200 m-u. PZ/ml. 2. Intracellular measurement of membrane potentials from the acinar cells of the same preparations showed that insulin (1 mu./ml.) simultaneously potentiates the hyperpolarization and amylase release in response to 0-5 m-u. PZ/ml. 3. These effects of insulin on the PZ-induced responses were inhibited by ouabain (3 X 10(-5) M). 4. The results suggest that insulin, in the concentration studied, has a potentiating action on the activity of an electrogenic Na pump, which maintains the hyperpolarization and amylase release during continuous stimulation with Pz. 5. Insulin in also potentiated the Pz-induced amylase release in the rat pancreata in situ after vagotomy and ligature of the pyloric region.", "PMID": 978585}
{"id": "pubmed23n0032_11797", "title": "Sialotonin: vasopressor substance in saliva and submandibular gland of the cat.", "content": "1. The large molecular pressor agent, sialotonin, present in cat saliva, has been separated from kallikrein and characterized further. 2. Sialotonin is present in saliva produced by parasympathetic (ch8da) but not in that produced by sympathetic nerve stimulation. After degenerative preganglionic parasympathetic nerve section, both sialotonin and kallikrein disappear completely, or nearly so. Sympathetic nerve section, however, fails to affect the concentration of these substances in chorda saliva. 3. The sialotonin activity is saliva is unaffected by ligation of Wharton's duct for 3--4 days whereas the kallikrein concentration is greatly reduced. 4. Although normally present in chorda saliva, sialotonin cannot be detected in aqueous extracts of the submandibular gland. It is present, however, in extracts of glands which have previously been subjected to prolonged sympathetic nerve stimulation or to ligation of the duct for 3-4 days. 5. The intravenous or close-aterial injection of sialotonin causes marked but brief reductions of blood flow in the submaxillary gland and intestine.", "contents": "Sialotonin: vasopressor substance in saliva and submandibular gland of the cat. 1. The large molecular pressor agent, sialotonin, present in cat saliva, has been separated from kallikrein and characterized further. 2. Sialotonin is present in saliva produced by parasympathetic (ch8da) but not in that produced by sympathetic nerve stimulation. After degenerative preganglionic parasympathetic nerve section, both sialotonin and kallikrein disappear completely, or nearly so. Sympathetic nerve section, however, fails to affect the concentration of these substances in chorda saliva. 3. The sialotonin activity is saliva is unaffected by ligation of Wharton's duct for 3--4 days whereas the kallikrein concentration is greatly reduced. 4. Although normally present in chorda saliva, sialotonin cannot be detected in aqueous extracts of the submandibular gland. It is present, however, in extracts of glands which have previously been subjected to prolonged sympathetic nerve stimulation or to ligation of the duct for 3-4 days. 5. The intravenous or close-aterial injection of sialotonin causes marked but brief reductions of blood flow in the submaxillary gland and intestine.", "PMID": 978586}
{"id": "pubmed23n0032_11798", "title": "Calcium and the control of discrete wave latency in the ventral photoreceptor of Limulus.", "content": "1. Discrete, transient depolarization (discrete waves) of the ventral photoreceptor of the horseshoe crab, Limulus, occur spontaneously in the dark adapted photoreceptor and are also evoked by light. They form the basic events which comprise the receptor potential. A brief, low energy flash of light evokes variable numbers of discrete waves which have variable latencies. Evidence suggesting that discrete wave latency reflects the kinetics of the chemical reactions of phototransduction is reviewed. 2. The concentration of extracellular Ca influences both the average discrete wave latency and its variability. Lowering extracellular Ca prolongs the latency and increases its variability. Increasing extracellular Ca has the opposite effect. 3. Changes in discrete wave latency caused by changes in extracellular Ca require 10--15 min to become fully manifest, whereas when the concentration of extracellular K is increased the photoreceptor achieves a steady-state depolarization in 10-15 sec. 4. Iontophoresis of the Ca-chelating agent EGTA into the photoreceptor increases both the average discrete wave latency and its variability. Iontophoresis of Ca-EGTA mixtures may either increase or decrease discrete wave latency and its variability depending upon the proportion of Ca mixed with EGTA. 5. It is suggested that the concentration of intracellular rather than extracellular ionized Ca is the prime factor indicating discrete wave latency. The effects of changing extracellular Ca can be explained if the photoreceptor is permeable to Ca in the dark and if it maintains a low intracellular Ca concentration by virtue of active metabolic processes (a pump-leak system). 6. Lowering the temperature of the photoreceptor also has the dual effect of increasing discrete wave latency and its variability. However, effects of lowering temperature and Ca simultaneously are greater than the sum of the two effects in individually. This suggests that Ca may be a reactant in the chemical process of phototransduction. 7. Changing the concentration of extracellular Ca does not change the quantum efficiency of discrete wave production. A previous study showed that quantum efficiency is not changed by temperature. Thus, once initiated by the absorption of light, the reactions that subserve phototransduction may be forced to completion. Ca probably exerts its influence by changing one or more rate constants in the reaction sequence.", "contents": "Calcium and the control of discrete wave latency in the ventral photoreceptor of Limulus. 1. Discrete, transient depolarization (discrete waves) of the ventral photoreceptor of the horseshoe crab, Limulus, occur spontaneously in the dark adapted photoreceptor and are also evoked by light. They form the basic events which comprise the receptor potential. A brief, low energy flash of light evokes variable numbers of discrete waves which have variable latencies. Evidence suggesting that discrete wave latency reflects the kinetics of the chemical reactions of phototransduction is reviewed. 2. The concentration of extracellular Ca influences both the average discrete wave latency and its variability. Lowering extracellular Ca prolongs the latency and increases its variability. Increasing extracellular Ca has the opposite effect. 3. Changes in discrete wave latency caused by changes in extracellular Ca require 10--15 min to become fully manifest, whereas when the concentration of extracellular K is increased the photoreceptor achieves a steady-state depolarization in 10-15 sec. 4. Iontophoresis of the Ca-chelating agent EGTA into the photoreceptor increases both the average discrete wave latency and its variability. Iontophoresis of Ca-EGTA mixtures may either increase or decrease discrete wave latency and its variability depending upon the proportion of Ca mixed with EGTA. 5. It is suggested that the concentration of intracellular rather than extracellular ionized Ca is the prime factor indicating discrete wave latency. The effects of changing extracellular Ca can be explained if the photoreceptor is permeable to Ca in the dark and if it maintains a low intracellular Ca concentration by virtue of active metabolic processes (a pump-leak system). 6. Lowering the temperature of the photoreceptor also has the dual effect of increasing discrete wave latency and its variability. However, effects of lowering temperature and Ca simultaneously are greater than the sum of the two effects in individually. This suggests that Ca may be a reactant in the chemical process of phototransduction. 7. Changing the concentration of extracellular Ca does not change the quantum efficiency of discrete wave production. A previous study showed that quantum efficiency is not changed by temperature. Thus, once initiated by the absorption of light, the reactions that subserve phototransduction may be forced to completion. Ca probably exerts its influence by changing one or more rate constants in the reaction sequence.", "PMID": 978587}
{"id": "pubmed23n0032_11799", "title": "The effects of irritation at various levels of the airway upon tracheal mucus secretion in the cat.", "content": "1. Sulphated glycoprotein output from the trachea, isolated in situ, has been measured in anaesthetized cats by a radio-isotopic method. The effects of irritation of various parts of the airway on this mucus output were studied. 2. Mechanical stimulation of the nose and nasopharynx increased tracheal mucus output by reflexes which involved parasympathetic and probably also sympathetic motor pathways. 3. Laryngeal stimulation had a similar through the same motor pathways. 4. Inhalation of ammonia vapour into the lower airways reflexly increased mucus output from the isolated trachea. The efferent pathway for this reflex was mainly or entirely parasympathetic. It is argued that the afferent pathway involved cough receptors. 5. Lung inflation, inhalation of histamine aerosol and intravenous injection of phenyl diguanide (which excite mainly lung stretch receptors, lung 'irritant' receptors and alveolar 'J-receptors' respectively) had no consistent effect on tracheal mucus secretion. 6. The afferent and efferent pathways of these reflexes are discussed.", "contents": "The effects of irritation at various levels of the airway upon tracheal mucus secretion in the cat. 1. Sulphated glycoprotein output from the trachea, isolated in situ, has been measured in anaesthetized cats by a radio-isotopic method. The effects of irritation of various parts of the airway on this mucus output were studied. 2. Mechanical stimulation of the nose and nasopharynx increased tracheal mucus output by reflexes which involved parasympathetic and probably also sympathetic motor pathways. 3. Laryngeal stimulation had a similar through the same motor pathways. 4. Inhalation of ammonia vapour into the lower airways reflexly increased mucus output from the isolated trachea. The efferent pathway for this reflex was mainly or entirely parasympathetic. It is argued that the afferent pathway involved cough receptors. 5. Lung inflation, inhalation of histamine aerosol and intravenous injection of phenyl diguanide (which excite mainly lung stretch receptors, lung 'irritant' receptors and alveolar 'J-receptors' respectively) had no consistent effect on tracheal mucus secretion. 6. The afferent and efferent pathways of these reflexes are discussed.", "PMID": 978588}
{"id": "pubmed23n0032_11800", "title": "Asymmetric tonic labyrinth reflexes and their interaction with neck reflexes in the decerebrate cat.", "content": "1. Tonic labyrinth and neck reflexes were studied separately and in combination in the decerebrate cat with C1 and C2 spinal roots cut. Reflex effects were observed as changes in length of the isotonically loaded medial head of triceps. 2. The tonic labyrinth reflexes acted asymmetrically on the medial head of triceps. Side-down rotation of the head produced shortening in medial triceps, whereas side-up rotations of the head resulted in a lengthening. 3. The tonic neck reflexes acted asymmetrically on the medial head of triceps. Side-down rotations of the neck produced a lengthening of medial triceps, whereas side-up rotations of the neck resulted in shortening. 4. Labyrinth and neck reflexes produce opposite effects on the same limb extensor muscle so that, if the neck innervation is intact, head tilting produces no change in muscle length. 5. It is suggested that the interaction between the labyrinth and neck reflexes contributes to the stability of the trunk, allowing the head to move freely on the body without affecting this stability. Labyrinth and neck reflexes need therefore to be considered together as a single system.", "contents": "Asymmetric tonic labyrinth reflexes and their interaction with neck reflexes in the decerebrate cat. 1. Tonic labyrinth and neck reflexes were studied separately and in combination in the decerebrate cat with C1 and C2 spinal roots cut. Reflex effects were observed as changes in length of the isotonically loaded medial head of triceps. 2. The tonic labyrinth reflexes acted asymmetrically on the medial head of triceps. Side-down rotation of the head produced shortening in medial triceps, whereas side-up rotations of the head resulted in a lengthening. 3. The tonic neck reflexes acted asymmetrically on the medial head of triceps. Side-down rotations of the neck produced a lengthening of medial triceps, whereas side-up rotations of the neck resulted in shortening. 4. Labyrinth and neck reflexes produce opposite effects on the same limb extensor muscle so that, if the neck innervation is intact, head tilting produces no change in muscle length. 5. It is suggested that the interaction between the labyrinth and neck reflexes contributes to the stability of the trunk, allowing the head to move freely on the body without affecting this stability. Labyrinth and neck reflexes need therefore to be considered together as a single system.", "PMID": 978589}
{"id": "pubmed23n0032_11801", "title": "Frequency of tendon organ discharges elicited by the contraction of motor units in cat leg muscles.", "content": "1. The responses elicited in individual tendon organs by the contraction of single motor units were studied in peroneus longus, peroneus brevis, tibialis anterior and soleus muscles. 2. No simple relation was found between the discharge frequency of a tendon organ and the tension produced in the muscle tendon by the contraction of individual motor units. 3. The sensitivity of a given tendon organ to contractile tension was not the same for each of the motor units which elicited its discharge. There was no correlation between the sensitivity of the receptor and the strength of the motor units. 4. Upon repetitive stimulation of a tendon-organ-activating motor unit at increasing rates, the frequency of the receptor sustained discharge reached a maximal value for rates of stimulation eliciting submaximal tetanic tension. Higher rates only produced an increase in the dynamic component of the tendon organ response. 5. These observations show that the contractile tension sensed by a tendon organ is not a simple fraction of the tension which appears at the muscle tendon. They might be accounted for as consequences of the fine structure of tendon organs and of variations in the number of muscle fibres contributed by different motor units to the bundle inserted on each receptor. The location of most tendon organs at musculo-aponeurotic junctions rather than in the tendon proper, could also be responsible for some of the observed discrepancies.", "contents": "Frequency of tendon organ discharges elicited by the contraction of motor units in cat leg muscles. 1. The responses elicited in individual tendon organs by the contraction of single motor units were studied in peroneus longus, peroneus brevis, tibialis anterior and soleus muscles. 2. No simple relation was found between the discharge frequency of a tendon organ and the tension produced in the muscle tendon by the contraction of individual motor units. 3. The sensitivity of a given tendon organ to contractile tension was not the same for each of the motor units which elicited its discharge. There was no correlation between the sensitivity of the receptor and the strength of the motor units. 4. Upon repetitive stimulation of a tendon-organ-activating motor unit at increasing rates, the frequency of the receptor sustained discharge reached a maximal value for rates of stimulation eliciting submaximal tetanic tension. Higher rates only produced an increase in the dynamic component of the tendon organ response. 5. These observations show that the contractile tension sensed by a tendon organ is not a simple fraction of the tension which appears at the muscle tendon. They might be accounted for as consequences of the fine structure of tendon organs and of variations in the number of muscle fibres contributed by different motor units to the bundle inserted on each receptor. The location of most tendon organs at musculo-aponeurotic junctions rather than in the tendon proper, could also be responsible for some of the observed discrepancies.", "PMID": 978590}
{"id": "pubmed23n0032_11802", "title": "Inhibitory effects of acetylcholine on neurones in the feline nucleus reticularis thalami.", "content": "1. Short iontophoretic pulses of acetylcholine (ACh) inhibited almost every spontaneously active cell encountered in the nucleus reticularis thalami of cats anaesthetized with a mixture of halothane, nitrous oxide and oxygen. On 200 cells the mean current needed to eject an effective inhibitory dose of ACh was 67 +/- 2 nA. When the ACh-evoked inhibition was mimicked by gamma-aminobutyric acid (GABA) or glycine on the same cell, the current required to release ACh was found to be approximately twice as great as that required to release an equally effective dose of GABA or glycine. 2. ACh inhibitions developed with a latency which was very much shorter than that for ACh excitation in cells of the ventrobasal complex. The latency of the ACh-evoked inhibition was as rapid as the onset and offset of the excitation of the same cells glutamate and their inhibition by GABA or glycine. 3. The firing pattern of ACh-inhibited neurones in the nucleus reticularis was characterized by periods of prolonged, high frequency bursts, and their mean firing frequency was 22 Hz. Raster dot displays and interspike interval histograms showed that whereas ACh suppressed the spikes that occurred between bursts much more readily than those that occurred during bursts, all spikes were equally sensitive to the depressant action of GABA and glycine. Large doses of ACh provoked or exaggerated burst activity. 4. ACh-evoked inhibition was extremely sensitive to blockade by short iontophoretic applications of atropine, which had no effect on the inhibitions evoked on the same cell equipotent doses of GABA or glycine. The ACh-evoked inhibitions were also antagonized by dihydro-beta-erythroidine released with slightly larger currents. When tested on the same cell, small iontophoretic applications of picrotoxin and bicuculline methoiodide blocked the inhibition evoked by GABA but had no effect on that evoked by ACh. Iontophoretic strychnine only rarely affected the inhibition evoked by ACh, while readily blocking the inhibition evoked on the same cell by an equipotent dose of glycine. In two cats, intravenous strychnine (1-2 mg/kg) had no effect on the ACh-evoked inhibition, while greatly reducing the sensitivity of the cell under study to glycine. 5. Only four out of forty-eight ACh-inhibted cells tested were inhibited by iontophoretic applications of either guanosine or adenosine 3':5'-phosphate. 6. Cells of the nucleus reticularis have been shown to have an inhibitory action on the thalamic relay cells, which are excited by ACh. It is suggested that the presence of both ACh excited and inhibited cells in different nuclei of the thalamus could be of considerable functional significance in gating sensory transmission through the thalamus.", "contents": "Inhibitory effects of acetylcholine on neurones in the feline nucleus reticularis thalami. 1. Short iontophoretic pulses of acetylcholine (ACh) inhibited almost every spontaneously active cell encountered in the nucleus reticularis thalami of cats anaesthetized with a mixture of halothane, nitrous oxide and oxygen. On 200 cells the mean current needed to eject an effective inhibitory dose of ACh was 67 +/- 2 nA. When the ACh-evoked inhibition was mimicked by gamma-aminobutyric acid (GABA) or glycine on the same cell, the current required to release ACh was found to be approximately twice as great as that required to release an equally effective dose of GABA or glycine. 2. ACh inhibitions developed with a latency which was very much shorter than that for ACh excitation in cells of the ventrobasal complex. The latency of the ACh-evoked inhibition was as rapid as the onset and offset of the excitation of the same cells glutamate and their inhibition by GABA or glycine. 3. The firing pattern of ACh-inhibited neurones in the nucleus reticularis was characterized by periods of prolonged, high frequency bursts, and their mean firing frequency was 22 Hz. Raster dot displays and interspike interval histograms showed that whereas ACh suppressed the spikes that occurred between bursts much more readily than those that occurred during bursts, all spikes were equally sensitive to the depressant action of GABA and glycine. Large doses of ACh provoked or exaggerated burst activity. 4. ACh-evoked inhibition was extremely sensitive to blockade by short iontophoretic applications of atropine, which had no effect on the inhibitions evoked on the same cell equipotent doses of GABA or glycine. The ACh-evoked inhibitions were also antagonized by dihydro-beta-erythroidine released with slightly larger currents. When tested on the same cell, small iontophoretic applications of picrotoxin and bicuculline methoiodide blocked the inhibition evoked by GABA but had no effect on that evoked by ACh. Iontophoretic strychnine only rarely affected the inhibition evoked by ACh, while readily blocking the inhibition evoked on the same cell by an equipotent dose of glycine. In two cats, intravenous strychnine (1-2 mg/kg) had no effect on the ACh-evoked inhibition, while greatly reducing the sensitivity of the cell under study to glycine. 5. Only four out of forty-eight ACh-inhibted cells tested were inhibited by iontophoretic applications of either guanosine or adenosine 3':5'-phosphate. 6. Cells of the nucleus reticularis have been shown to have an inhibitory action on the thalamic relay cells, which are excited by ACh. It is suggested that the presence of both ACh excited and inhibited cells in different nuclei of the thalamus could be of considerable functional significance in gating sensory transmission through the thalamus.", "PMID": 978591}
{"id": "pubmed23n0032_11803", "title": "The incidence of myocardial infarction.", "content": "I studied 183 patients with myocardial infarction including sudden deaths related to ischaemic heart disease occurring in general practice during a period of four years.The figures are examined by age, sex, history of event, and place of treatment. The ratio of male to female infarctions was 2:1, males having a mean age of 63 years and females 71 years.The total death rate for all myocardial infarctions was 46 per cent, but if sudden deaths are excluded, the rate becomes 24 per cent.The annual incidence of myocardial infarction per 1,000 in the practice population of 11,195 was 4.1 and the total annual death rate per 1,000 was 1.9.", "contents": "The incidence of myocardial infarction. I studied 183 patients with myocardial infarction including sudden deaths related to ischaemic heart disease occurring in general practice during a period of four years.The figures are examined by age, sex, history of event, and place of treatment. The ratio of male to female infarctions was 2:1, males having a mean age of 63 years and females 71 years.The total death rate for all myocardial infarctions was 46 per cent, but if sudden deaths are excluded, the rate becomes 24 per cent.The annual incidence of myocardial infarction per 1,000 in the practice population of 11,195 was 4.1 and the total annual death rate per 1,000 was 1.9.", "PMID": 978639}
{"id": "pubmed23n0032_11804", "title": "The natural history of angina in a general practice.", "content": "An appreciation of the natural history of angina pectoris is important when deciding on the place of new and potentially dangerous forms of treatment. During 1950-1975, 268 patients with angina were diagnosed and followed up in my London general practice. The annual incidence, in adults over 40, was five per 1,000 and increased with age.During the period of follow-up, half the patients died, an annual mortality of 4.6 per cent. However, among the survivors one third ceased to suffer anginal symptoms spontaneously and without specific therapy. Of those who continued to suffer from angina, in 71 per cent the condition was graded as minor, in 27 per cent as moderate, and in only two per cent were the attacks severe and disabling. Usually the angina was primary (77 per cent) and it was secondary, after myocardial infarction, in 23 per cent.Of the 134 deaths, three quarters were from a cardiovascular cause. This group of angina patients had a 2:1 times greater observed, than expected, risk of dying (O/E ratio). The O/E mortality ratio fell progressively with age. It was highest in the 40-49 decade (4.0) and lowest in the over 80s, when the observed mortality rate was less than expected (0.9). The O/E mortality ratio was higher in men (2.3) than in women (1.7).From this survey I conclude that angina does not have a uniformly bad prognosis, and that with the advent of beta-adrenergic blockers, the proportion considered for angiocardiography and aorto-coronary bypass grafting should be less than five per cent of all patients with angina.", "contents": "The natural history of angina in a general practice. An appreciation of the natural history of angina pectoris is important when deciding on the place of new and potentially dangerous forms of treatment. During 1950-1975, 268 patients with angina were diagnosed and followed up in my London general practice. The annual incidence, in adults over 40, was five per 1,000 and increased with age.During the period of follow-up, half the patients died, an annual mortality of 4.6 per cent. However, among the survivors one third ceased to suffer anginal symptoms spontaneously and without specific therapy. Of those who continued to suffer from angina, in 71 per cent the condition was graded as minor, in 27 per cent as moderate, and in only two per cent were the attacks severe and disabling. Usually the angina was primary (77 per cent) and it was secondary, after myocardial infarction, in 23 per cent.Of the 134 deaths, three quarters were from a cardiovascular cause. This group of angina patients had a 2:1 times greater observed, than expected, risk of dying (O/E ratio). The O/E mortality ratio fell progressively with age. It was highest in the 40-49 decade (4.0) and lowest in the over 80s, when the observed mortality rate was less than expected (0.9). The O/E mortality ratio was higher in men (2.3) than in women (1.7).From this survey I conclude that angina does not have a uniformly bad prognosis, and that with the advent of beta-adrenergic blockers, the proportion considered for angiocardiography and aorto-coronary bypass grafting should be less than five per cent of all patients with angina.", "PMID": 978640}
{"id": "pubmed23n0032_11805", "title": "Analysis of time intervals involved in admission to a coronary care unit.", "content": "Time intervals between the onset of the presenting symptom (chest pain) and arrival in a coronary care unit were studied for 221 admissions arranged by conventional means. The median figure for \"patient delay\" was 60 minutes, for \"general-practitioner delay\" 20 minutes, for \"ambulance delay\" 30 minutes, and for \"transit delay\" 30 minutes. The median \"total delay\" was three hours 30 minutes.Only 4.5 per cent of the patients were under intensive coronary care within one hour, the time of the highest mortality risk. A mobile coronary service should be capable of increasing the proportion of patients brought under special care within the first hour, but the time taken by the patient to realise the nature of the emergency and summon aid is likely to remain the most critical factor.", "contents": "Analysis of time intervals involved in admission to a coronary care unit. Time intervals between the onset of the presenting symptom (chest pain) and arrival in a coronary care unit were studied for 221 admissions arranged by conventional means. The median figure for \"patient delay\" was 60 minutes, for \"general-practitioner delay\" 20 minutes, for \"ambulance delay\" 30 minutes, and for \"transit delay\" 30 minutes. The median \"total delay\" was three hours 30 minutes.Only 4.5 per cent of the patients were under intensive coronary care within one hour, the time of the highest mortality risk. A mobile coronary service should be capable of increasing the proportion of patients brought under special care within the first hour, but the time taken by the patient to realise the nature of the emergency and summon aid is likely to remain the most critical factor.", "PMID": 978641}
{"id": "pubmed23n0032_11806", "title": "A general-practitioner ward in a new district general hospital.", "content": "So far there are relatively few general-practitioner wards in district general hospitals in the National Health Service. The work of one such general-practitioner ward at Queen Mary's Hospital, Sidcup, is described and the advantages of this system of care for patients and doctors discussed.", "contents": "A general-practitioner ward in a new district general hospital. So far there are relatively few general-practitioner wards in district general hospitals in the National Health Service. The work of one such general-practitioner ward at Queen Mary's Hospital, Sidcup, is described and the advantages of this system of care for patients and doctors discussed.", "PMID": 978642}
{"id": "pubmed23n0032_11807", "title": "Attaching community psychiatric nurses to general practice.", "content": "A scheme for attachment of psychiatric nurses from hospital to group practice in Oxford is described. One community psychiatric nurse can work satisfactorily with eight general practitioners covering a population of about 18,000. From analysis of a working year, it is concluded that this arrangement improves the care of patients in the community by providing psychiatric help at times when it was previously unavailable or unacceptable.The implications of such a scheme for the workings of the primary health care team and the hospital psychiatric service are considered, and a case is made for a further study involving a comparison between practices with and without the attachment of a community psychiatric nurse.", "contents": "Attaching community psychiatric nurses to general practice. A scheme for attachment of psychiatric nurses from hospital to group practice in Oxford is described. One community psychiatric nurse can work satisfactorily with eight general practitioners covering a population of about 18,000. From analysis of a working year, it is concluded that this arrangement improves the care of patients in the community by providing psychiatric help at times when it was previously unavailable or unacceptable.The implications of such a scheme for the workings of the primary health care team and the hospital psychiatric service are considered, and a case is made for a further study involving a comparison between practices with and without the attachment of a community psychiatric nurse.", "PMID": 978643}
{"id": "pubmed23n0032_11808", "title": "Seeing two doctors at once in general practice.", "content": "We report the reactions of 250 patients who saw two doctors together, a general-practitioner trainer and a vocational trainee, when they came to a general practice for consultation.Over 80 per cent were neutral and the remainder were almost equally divided between those who preferred to see two doctors and those who preferred to see their own doctor alone.Selected favourable statements outnumbered adverse comments by about ten to one, although allowance must be made for the desire of patients to please their doctor.", "contents": "Seeing two doctors at once in general practice. We report the reactions of 250 patients who saw two doctors together, a general-practitioner trainer and a vocational trainee, when they came to a general practice for consultation.Over 80 per cent were neutral and the remainder were almost equally divided between those who preferred to see two doctors and those who preferred to see their own doctor alone.Selected favourable statements outnumbered adverse comments by about ten to one, although allowance must be made for the desire of patients to please their doctor.", "PMID": 978644}
{"id": "pubmed23n0032_11809", "title": "Using the first consultation in acute illness for teaching third year medical students.", "content": "We have introduced clinical teaching in general practice for students in their first clinical year. The students are present at the patient's initial consultation for an acute illness and thus see a wide range of morbidity which is seldom found in hospital. Log books are used to record such factors as the patient's presenting complaint, the diagnosis that is made and the presence of significant social and psychological aspects of the physical condition. Although there were only four two-hour teaching sessions, the frequency and the range of presenting complaints seen by students approximates to that in general practice. The opinions of both the tutors and the students to this form of teaching have been favourable.", "contents": "Using the first consultation in acute illness for teaching third year medical students. We have introduced clinical teaching in general practice for students in their first clinical year. The students are present at the patient's initial consultation for an acute illness and thus see a wide range of morbidity which is seldom found in hospital. Log books are used to record such factors as the patient's presenting complaint, the diagnosis that is made and the presence of significant social and psychological aspects of the physical condition. Although there were only four two-hour teaching sessions, the frequency and the range of presenting complaints seen by students approximates to that in general practice. The opinions of both the tutors and the students to this form of teaching have been favourable.", "PMID": 978645}
{"id": "pubmed23n0032_11810", "title": "The university department of general practice: its function and role.", "content": "The need is stressed for university departments of general practice to examine their role and create a philosophy which will guide their function. The success of a department depends upon clear objectives, teamwork, and integration with the teaching given by other clinical departments in the medical school. The contribution of general practice to undergraduate training is discussed and problems associated with the recruitment of staff are indicated.", "contents": "The university department of general practice: its function and role. The need is stressed for university departments of general practice to examine their role and create a philosophy which will guide their function. The success of a department depends upon clear objectives, teamwork, and integration with the teaching given by other clinical departments in the medical school. The contribution of general practice to undergraduate training is discussed and problems associated with the recruitment of staff are indicated.", "PMID": 978646}
{"id": "pubmed23n0032_11811", "title": "Screening the elderly in general practice.", "content": "All the 855 patients over the age of 65 in one general practice were reviewed. Those under care were excluded (316-37 per cent), and the rest were offered a screening examination in the practice. In all, 335 were examined and several had been previously screened. The yield of conditions found is reported and the advantage of the work in general practice discussed.", "contents": "Screening the elderly in general practice. All the 855 patients over the age of 65 in one general practice were reviewed. Those under care were excluded (316-37 per cent), and the rest were offered a screening examination in the practice. In all, 335 were examined and several had been previously screened. The yield of conditions found is reported and the advantage of the work in general practice discussed.", "PMID": 978648}
{"id": "pubmed23n0032_11812", "title": "Two knots in the umbilical cord.", "content": "A case of two true knots occurring in an umbilical cord is reported because of its relative rarity. The literature is reviewed. The cause of this potentially dangerous phenomenon is discussed, and a recommendation for further study made.", "contents": "Two knots in the umbilical cord. A case of two true knots occurring in an umbilical cord is reported because of its relative rarity. The literature is reviewed. The cause of this potentially dangerous phenomenon is discussed, and a recommendation for further study made.", "PMID": 978649}
{"id": "pubmed23n0032_11813", "title": "An approach to general oncology: principles and procedures.", "content": "The obstetrician-gynecologist is frequently the only physician to attend women during their reproductive years. Malignant disease outside the pelvic organs is a fairly frequent occurence in this age group. Certain findings in the patient's history and physical examination can suggest malignant disease. The routine laboratory examination can also provide indications of the presence of a neoplastic process. Once this process is suspected, histologic proof of malignancy must be obtained before further staging and therapy are considered. The extent of the disease is important for the planning of therapy, i.e., whether it is to be surgery, radiotherapy, chemotherapy or some combination of the three. Adjuvant chemotherapy is recognized as a form of treatment designed to eradicate micrometastases, prevent the occurrence of subsequent clinical metastatic disease and, as a result, improve survival.", "contents": "An approach to general oncology: principles and procedures. The obstetrician-gynecologist is frequently the only physician to attend women during their reproductive years. Malignant disease outside the pelvic organs is a fairly frequent occurence in this age group. Certain findings in the patient's history and physical examination can suggest malignant disease. The routine laboratory examination can also provide indications of the presence of a neoplastic process. Once this process is suspected, histologic proof of malignancy must be obtained before further staging and therapy are considered. The extent of the disease is important for the planning of therapy, i.e., whether it is to be surgery, radiotherapy, chemotherapy or some combination of the three. Adjuvant chemotherapy is recognized as a form of treatment designed to eradicate micrometastases, prevent the occurrence of subsequent clinical metastatic disease and, as a result, improve survival.", "PMID": 978651}
{"id": "pubmed23n0032_11814", "title": "Malignant lymphoma: implications during the reproductive years and pregnancy.", "content": "The malignant lymphomas are reviewed, and involvement of urogenital-ridge derivatives, including the reproductive organs, is summarized. Implications of therapy for pelvic lymphoma are discussed. It is shown by a retrospective analysis that Hodgkin's disease has little effect on fertility, the course of gestation, delivery or fetal wastage and that maternal death is not increased. No adverse effect of pregnancy on the symptoms or longevity of women with Hodgkin's disease can be demonstrated. Women diagnosed in pregnancy as having lymphoma should undergo therapeutic abortion so that proper staging and therapy may be given. Pregnancies over 32 to 34 weeks should be induced. Women previously treated for lymphoma may become infertile as a result of therapy. Those not infertile after therapy should avoid pregnancies since there is a potential risk of malformations and malignancies in the offspring.", "contents": "Malignant lymphoma: implications during the reproductive years and pregnancy. The malignant lymphomas are reviewed, and involvement of urogenital-ridge derivatives, including the reproductive organs, is summarized. Implications of therapy for pelvic lymphoma are discussed. It is shown by a retrospective analysis that Hodgkin's disease has little effect on fertility, the course of gestation, delivery or fetal wastage and that maternal death is not increased. No adverse effect of pregnancy on the symptoms or longevity of women with Hodgkin's disease can be demonstrated. Women diagnosed in pregnancy as having lymphoma should undergo therapeutic abortion so that proper staging and therapy may be given. Pregnancies over 32 to 34 weeks should be induced. Women previously treated for lymphoma may become infertile as a result of therapy. Those not infertile after therapy should avoid pregnancies since there is a potential risk of malformations and malignancies in the offspring.", "PMID": 978652}
{"id": "pubmed23n0032_11815", "title": "Thyroid cancer: relationship to radiation exposure and to pregnancy.", "content": "Exposure to radiation results in an increased occurrence of nodularity to the thyroid and, more important, the development of cancer in a significant proportion of patients. Near-total thyroidectomy is recommended in those patients with a history of irradiation who are found on physical examination of the thyroid to have one or more nodules. Although pregnancy appears to have no effect on the course of thyroid carcinoma and the tumor has no effect on pregnancy, because of the numerous stimuli to thyroid growth during pregnancy, we feel that pregnancy is best avoided by women with known residual disease.", "contents": "Thyroid cancer: relationship to radiation exposure and to pregnancy. Exposure to radiation results in an increased occurrence of nodularity to the thyroid and, more important, the development of cancer in a significant proportion of patients. Near-total thyroidectomy is recommended in those patients with a history of irradiation who are found on physical examination of the thyroid to have one or more nodules. Although pregnancy appears to have no effect on the course of thyroid carcinoma and the tumor has no effect on pregnancy, because of the numerous stimuli to thyroid growth during pregnancy, we feel that pregnancy is best avoided by women with known residual disease.", "PMID": 978653}
{"id": "pubmed23n0032_11816", "title": "Acute leukemia during reproductive life: its course, complications and sequelae for fertility.", "content": "Acute leukemia is less common during the reproductive years than in children or in post-menopausal women. Effective chemotherapy exists for adult lymphocytic leukemia, and the median survival is 18 to 20 months. Acute myelogenous leukemia still has a less favorable prognosis, with a medial survival of 12 months despite effective chemotherapeutic agents. The occurrence of acute leukemia in pregnancy does not change the overall prognosis, which depends primarily on the cytopathologic types. If leukemia occurs during the first trimester, therapeutic abortion is advised since the rate of spontaneous abortion after chemotherapy is high in the first trimester and fetal malformations are common. Acute leukemia can be treated in the second and third trimesters with little effect on the pregnancy or fetus. In patients cured of acute leukemia, the potential for subsequent pregnancies exists with little likelihood of increases in fetal malformations.", "contents": "Acute leukemia during reproductive life: its course, complications and sequelae for fertility. Acute leukemia is less common during the reproductive years than in children or in post-menopausal women. Effective chemotherapy exists for adult lymphocytic leukemia, and the median survival is 18 to 20 months. Acute myelogenous leukemia still has a less favorable prognosis, with a medial survival of 12 months despite effective chemotherapeutic agents. The occurrence of acute leukemia in pregnancy does not change the overall prognosis, which depends primarily on the cytopathologic types. If leukemia occurs during the first trimester, therapeutic abortion is advised since the rate of spontaneous abortion after chemotherapy is high in the first trimester and fetal malformations are common. Acute leukemia can be treated in the second and third trimesters with little effect on the pregnancy or fetus. In patients cured of acute leukemia, the potential for subsequent pregnancies exists with little likelihood of increases in fetal malformations.", "PMID": 978654}
{"id": "pubmed23n0032_11817", "title": "Consequences of radiotherapy and antineoplastic therapy for the fetus.", "content": "The consequences of radiation and antineoplastic therapy on the fetus are summarized. Animal and atom bomb studies clearly demonstrate the carcinogenic effects of radiation. Less information is available on neoplasia and neonatal malformations induced by chemotherapy, but there may be an increased risk. The delayed consequences of radiation and chemotherapy are not well known and require further study.", "contents": "Consequences of radiotherapy and antineoplastic therapy for the fetus. The consequences of radiation and antineoplastic therapy on the fetus are summarized. Animal and atom bomb studies clearly demonstrate the carcinogenic effects of radiation. Less information is available on neoplasia and neonatal malformations induced by chemotherapy, but there may be an increased risk. The delayed consequences of radiation and chemotherapy are not well known and require further study.", "PMID": 978655}
{"id": "pubmed23n0032_11818", "title": "Dermatomyositis following rhabdomyolysis.", "content": "A case is presented, in which classic dermatomyositis occurred six weeks after documented rhabdomyolysis. The literature concerning the immunopathogenesis of dermatomyositis is briefly reviewed.", "contents": "Dermatomyositis following rhabdomyolysis. A case is presented, in which classic dermatomyositis occurred six weeks after documented rhabdomyolysis. The literature concerning the immunopathogenesis of dermatomyositis is briefly reviewed.", "PMID": 978661}
{"id": "pubmed23n0032_11819", "title": "Polymyositis in a patient with multiple neoplasms.", "content": "A 64 year old woman with a past history of panhysterectomy and oophorectomy for carcinoma of the body of the uterus (1950) and partial colectomy for carcinoma of the colon (1971), presented in 1972 with severe weakness of the proximal girdle muscles and histological evidence of polymyositis. A detailed search disclosed no tumor and she was treated with prednisone. Two years later, investigations for iron deficiency anemia revealed two polyps in the colon. Pathological examination of the resected colon disclosed two separate foci of adenocarcinoma and a number of adenomatous polyps. Three months later, further investigations for melena led to the discovery of a gastric carcinoma. Due to the extent of the tumor, she was subjected to gastrectomy. splenectomy, and excision of the tail of the pancreas, but died of postoperative complications. At autopsy, no residual cancer was detected, but a meningioma was found. The association of polymyositis with malignant tumors has been recognized for a long time, but only two previously reported patients have had more than one cancer. Although a causal relationship is difficult to establish, continued vigilance for neoplasms is advocated during the follow-up period.", "contents": "Polymyositis in a patient with multiple neoplasms. A 64 year old woman with a past history of panhysterectomy and oophorectomy for carcinoma of the body of the uterus (1950) and partial colectomy for carcinoma of the colon (1971), presented in 1972 with severe weakness of the proximal girdle muscles and histological evidence of polymyositis. A detailed search disclosed no tumor and she was treated with prednisone. Two years later, investigations for iron deficiency anemia revealed two polyps in the colon. Pathological examination of the resected colon disclosed two separate foci of adenocarcinoma and a number of adenomatous polyps. Three months later, further investigations for melena led to the discovery of a gastric carcinoma. Due to the extent of the tumor, she was subjected to gastrectomy. splenectomy, and excision of the tail of the pancreas, but died of postoperative complications. At autopsy, no residual cancer was detected, but a meningioma was found. The association of polymyositis with malignant tumors has been recognized for a long time, but only two previously reported patients have had more than one cancer. Although a causal relationship is difficult to establish, continued vigilance for neoplasms is advocated during the follow-up period.", "PMID": 978662}
{"id": "pubmed23n0032_11820", "title": "Classical rheumatoid arthritis in a patient with Reiter's syndrome.", "content": "A 44 year old white male presented with sterile urethritis, anterior uveitis, painless oral ulcers, low back pain, and synovitis of the knees and left ankle. Unilateral sacroiliitis and limited spondylitis further supported a diagnosis of Reiter's syndrome. Serum rheumatoid factor was present. The patient subsequently developed morning stiffness, symmetrical polyarthritis of the hands and wrists, and an olecranon subcutaneous nodule. Histology of the nodule and synovium was consistent with rheumatoid arthritis. Histocompatibility antigen typing established the presence of HLA B27. The occurrence of rheumatoid arthritis and Reiter's syndrome in the same patient is quite rare, and the infrequent co-existence of these two inflammatory rheumatic diseases is discussed.", "contents": "Classical rheumatoid arthritis in a patient with Reiter's syndrome. A 44 year old white male presented with sterile urethritis, anterior uveitis, painless oral ulcers, low back pain, and synovitis of the knees and left ankle. Unilateral sacroiliitis and limited spondylitis further supported a diagnosis of Reiter's syndrome. Serum rheumatoid factor was present. The patient subsequently developed morning stiffness, symmetrical polyarthritis of the hands and wrists, and an olecranon subcutaneous nodule. Histology of the nodule and synovium was consistent with rheumatoid arthritis. Histocompatibility antigen typing established the presence of HLA B27. The occurrence of rheumatoid arthritis and Reiter's syndrome in the same patient is quite rare, and the infrequent co-existence of these two inflammatory rheumatic diseases is discussed.", "PMID": 978663}
{"id": "pubmed23n0032_11821", "title": "Rheumatoid pericarditis: comparison of immunologic characteristics of pericardial fluid, synovial fluid, and serum.", "content": "A comparison was made of the immunological characteristics of serum, synovial and pericardial fluids obtained from a 54 year old man with classic rheumatoid arthritis and pericarditis. The synovial and pericardial fluids had low complement levels and immune complexes were detected using both biological and physical methods. The serum had normal complement levels and no immune complexes could be demonstrated. The presence of immune complexes and complement depletion in the synovial and pericardial fluids supports the concept of local production of immune complexes, and is in keeping with a Type III mechanism of immunologic injury.", "contents": "Rheumatoid pericarditis: comparison of immunologic characteristics of pericardial fluid, synovial fluid, and serum. A comparison was made of the immunological characteristics of serum, synovial and pericardial fluids obtained from a 54 year old man with classic rheumatoid arthritis and pericarditis. The synovial and pericardial fluids had low complement levels and immune complexes were detected using both biological and physical methods. The serum had normal complement levels and no immune complexes could be demonstrated. The presence of immune complexes and complement depletion in the synovial and pericardial fluids supports the concept of local production of immune complexes, and is in keeping with a Type III mechanism of immunologic injury.", "PMID": 978664}
{"id": "pubmed23n0032_11822", "title": "Acute leukemia in rheumatoid arthritis treated with cytotoxic agents.", "content": "Acute leukemia is described in two patients treated with cytotoxic agents for a destructive, seropositive rheumatoid arthritis. Both patients had received longterm azathioprine therapy. In addition, one patient had been treated with cyclophosphamide, the other with melphalan. Chromosomal abnormalities were noted in both patients. Studies in one patient included colony forming units, ferrokinetics, electron microscopy of bone marrow, and autopsy examination. All reports of acute leukemia associated with cytostatic drugs in the literature to date are reviewed and the possible mechanisms discussed. It is suggested that patients with rheumatoid arthritis treated with azathioprine and alkylating agents may have an increased risk of developing a therapy-related acute leukemia.", "contents": "Acute leukemia in rheumatoid arthritis treated with cytotoxic agents. Acute leukemia is described in two patients treated with cytotoxic agents for a destructive, seropositive rheumatoid arthritis. Both patients had received longterm azathioprine therapy. In addition, one patient had been treated with cyclophosphamide, the other with melphalan. Chromosomal abnormalities were noted in both patients. Studies in one patient included colony forming units, ferrokinetics, electron microscopy of bone marrow, and autopsy examination. All reports of acute leukemia associated with cytostatic drugs in the literature to date are reviewed and the possible mechanisms discussed. It is suggested that patients with rheumatoid arthritis treated with azathioprine and alkylating agents may have an increased risk of developing a therapy-related acute leukemia.", "PMID": 978665}
{"id": "pubmed23n0032_11823", "title": "Pharmacokinetic and clinical studies of a new uricosuric agent - benzbromarone.", "content": "Benzbromarone, a potent uricosuric agent, is a benzofuran derivative with a bromine on the 3rd and 5th positions of the benzene ring. Readily absorbed after oral administration, it is promptly dehalogenated in the liver and excreted via the biliary system. Peak drug concentration usually precedes maximal uricosuria following a single dose of 40 mg of benzbromarone, since benzarone, one of the two metabolites, likewise has a uricosuric action. Longterm studies in 24 gouty patients indicate that the drug is well tolerated. It has not produced any skin rash or renal colic. Renal hemodynamics, blood picture, and liver enzymes were unchanged. Since it is eliminated by the biliary tract, it may cause diarrhoea in some patients. Being a very potent uricosuric agent, it is not advocated in patients with a history of uric acid lithiasis. The uricosuric effect is not liable to be counteracted when used in conjunction with hyperuricemic diuretics. The drug is particularly useful in patients with chronic gouty arthritis and tophi, either refractory or allergic to probenecid, sulfinpyrazone, or allopurinol.", "contents": "Pharmacokinetic and clinical studies of a new uricosuric agent - benzbromarone. Benzbromarone, a potent uricosuric agent, is a benzofuran derivative with a bromine on the 3rd and 5th positions of the benzene ring. Readily absorbed after oral administration, it is promptly dehalogenated in the liver and excreted via the biliary system. Peak drug concentration usually precedes maximal uricosuria following a single dose of 40 mg of benzbromarone, since benzarone, one of the two metabolites, likewise has a uricosuric action. Longterm studies in 24 gouty patients indicate that the drug is well tolerated. It has not produced any skin rash or renal colic. Renal hemodynamics, blood picture, and liver enzymes were unchanged. Since it is eliminated by the biliary tract, it may cause diarrhoea in some patients. Being a very potent uricosuric agent, it is not advocated in patients with a history of uric acid lithiasis. The uricosuric effect is not liable to be counteracted when used in conjunction with hyperuricemic diuretics. The drug is particularly useful in patients with chronic gouty arthritis and tophi, either refractory or allergic to probenecid, sulfinpyrazone, or allopurinol.", "PMID": 978666}
{"id": "pubmed23n0032_11824", "title": "Postoperative heterotopic ossification in patients with ankylosing hyperostosis on the spine (Forestier's disease).", "content": "Heterotopic ossification following hip surgery occurred in three patients with ankylosing hyperostosis of the spine. No technical difficulty during surgery was encountered in these individuals. The occurrence of this postoperative complication, coupled with the appearance of bony outgrowths at sites of ligament attachment throughout the axial and extra-axial skeleton in patients with ankylosing hyperostosis of the spine, suggests the presence of an underlying ossifying diathesis, diffuse idiopathic skeletal hyperostosis (DISH). A significant number of patients with DISH possess the second segregant series antigen, HLA-B27, a feature they share with individuals with other arthropathies characterized by abundant ossification; this gene may be closely related to one which influences bone formation. The possible association of postoperative heterotopic ossification and ankylosing hyperostosis of the spine indicates that a radiographic examination of the vertebral column in patients undergoing hip surgery may be a useful screening procedure.", "contents": "Postoperative heterotopic ossification in patients with ankylosing hyperostosis on the spine (Forestier's disease). Heterotopic ossification following hip surgery occurred in three patients with ankylosing hyperostosis of the spine. No technical difficulty during surgery was encountered in these individuals. The occurrence of this postoperative complication, coupled with the appearance of bony outgrowths at sites of ligament attachment throughout the axial and extra-axial skeleton in patients with ankylosing hyperostosis of the spine, suggests the presence of an underlying ossifying diathesis, diffuse idiopathic skeletal hyperostosis (DISH). A significant number of patients with DISH possess the second segregant series antigen, HLA-B27, a feature they share with individuals with other arthropathies characterized by abundant ossification; this gene may be closely related to one which influences bone formation. The possible association of postoperative heterotopic ossification and ankylosing hyperostosis of the spine indicates that a radiographic examination of the vertebral column in patients undergoing hip surgery may be a useful screening procedure.", "PMID": 978667}
{"id": "pubmed23n0032_11825", "title": "Radiologic and scintiscan findings in HLA-B27 negative patients with ankylosing spondylitis.", "content": "Of 189 consecutive patients seen with definite ankylosing spondylitis, 17 did not possess the transplantation antigen HLA-B27. These 17 patients had less severe radiological evidence of disease than the group as a whole, or a sub-group matched for age, sex, and duration of disease. This supports the suggestion that the HLS-B27 antigen itself may play a role in the pathogenesis of the disease, and does not serve merely as a marker for a linked immune response gene.", "contents": "Radiologic and scintiscan findings in HLA-B27 negative patients with ankylosing spondylitis. Of 189 consecutive patients seen with definite ankylosing spondylitis, 17 did not possess the transplantation antigen HLA-B27. These 17 patients had less severe radiological evidence of disease than the group as a whole, or a sub-group matched for age, sex, and duration of disease. This supports the suggestion that the HLS-B27 antigen itself may play a role in the pathogenesis of the disease, and does not serve merely as a marker for a linked immune response gene.", "PMID": 978668}
{"id": "pubmed23n0032_11826", "title": "Stereochemical studies on medicinal agents. 20. Absolute configuration and analgetic potency alpha-promedol enantiomers. The role of the C-4 chiral center in conferring stereoselectivity in axial- and equatorial-phenyl prodine congeners.", "content": "Opical antipodes of the axial phenyl analgetic, alpha-promedol hydrochloride (3), were prepared and the absolute stereochemistry wasd determined by relating one of the enantiomers to its gamme diastereomer having the 2S, 4S,5R configuration. The analegetic potency of (+)-(2R,4S,5S)-3 is 20 times that of morphine, while its enantiomer, (-)-(2S,4R,5R)-3, is inactive at 50 mg/kg. These results are in accord with prior reports which indicate that substitution of a 3-or 5-alkyl group on the pro-4S enantiotopic edge of the piperidine ring leads to enantiomers which have greater potency than those substituted in an identical position on the pro-4R edge. This coupled with the fact that the torsion angle between the axial phenyl group and piperidine ring in (+)-3 is of the same sign as its equatorial congeners, suggests that the C(3)-C(4)-C(5) moiety and its substituents at C(4) are located in a similar chiral environment on the receptor. In contrast, the C(2)-N-C(6) portion of the axial and equatorial molecules does not bind in the same receptor environment, and it is suggested that different modes of interaction in the prodine series arise from different orientations of this moiety.", "contents": "Stereochemical studies on medicinal agents. 20. Absolute configuration and analgetic potency alpha-promedol enantiomers. The role of the C-4 chiral center in conferring stereoselectivity in axial- and equatorial-phenyl prodine congeners. Opical antipodes of the axial phenyl analgetic, alpha-promedol hydrochloride (3), were prepared and the absolute stereochemistry wasd determined by relating one of the enantiomers to its gamme diastereomer having the 2S, 4S,5R configuration. The analegetic potency of (+)-(2R,4S,5S)-3 is 20 times that of morphine, while its enantiomer, (-)-(2S,4R,5R)-3, is inactive at 50 mg/kg. These results are in accord with prior reports which indicate that substitution of a 3-or 5-alkyl group on the pro-4S enantiotopic edge of the piperidine ring leads to enantiomers which have greater potency than those substituted in an identical position on the pro-4R edge. This coupled with the fact that the torsion angle between the axial phenyl group and piperidine ring in (+)-3 is of the same sign as its equatorial congeners, suggests that the C(3)-C(4)-C(5) moiety and its substituents at C(4) are located in a similar chiral environment on the receptor. In contrast, the C(2)-N-C(6) portion of the axial and equatorial molecules does not bind in the same receptor environment, and it is suggested that different modes of interaction in the prodine series arise from different orientations of this moiety.", "PMID": 978670}
{"id": "pubmed23n0032_11827", "title": "Irreversible enzyme inhibitors. Inhibitors of guinea pig complement derived by quaternization of substituted pyridines with benzyl halides.", "content": "A series of 83 compounds derived from hydrocarbon-substituted pyridines by quaternization with PhCH2Br usually containing a 2-SO2F or 6-Cl-2-SO2F group was synthesized and evaluated as inhibitors of guinea pig complement and in most cases its C1 component. The most active compounds were 3-(4-phenylphenylbutyl)-N-(6-choro-2-fluorosulfonylbenzyl) pyridinium bromide (43) and 3-(4-phenylphenylbutyl)-N-(2-fluorosulfonylbenzyl) pyridinium bromide (44), each showing 50% inhibition at 7.8 muM. The most effective irreversible inhibitor of the C1 component was N-(6-chloro-2-fluorosulfonylbenzyl)-5,6-benzoquinolinium bromide (87), which showed 50% inhibition at 4 muM.", "contents": "Irreversible enzyme inhibitors. Inhibitors of guinea pig complement derived by quaternization of substituted pyridines with benzyl halides. A series of 83 compounds derived from hydrocarbon-substituted pyridines by quaternization with PhCH2Br usually containing a 2-SO2F or 6-Cl-2-SO2F group was synthesized and evaluated as inhibitors of guinea pig complement and in most cases its C1 component. The most active compounds were 3-(4-phenylphenylbutyl)-N-(6-choro-2-fluorosulfonylbenzyl) pyridinium bromide (43) and 3-(4-phenylphenylbutyl)-N-(2-fluorosulfonylbenzyl) pyridinium bromide (44), each showing 50% inhibition at 7.8 muM. The most effective irreversible inhibitor of the C1 component was N-(6-chloro-2-fluorosulfonylbenzyl)-5,6-benzoquinolinium bromide (87), which showed 50% inhibition at 4 muM.", "PMID": 978671}
{"id": "pubmed23n0032_11828", "title": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 4. Further modifications of the amino and base portions of S-adenosyl-L-homocysteine.", "content": "Structural analogues of S-adenosyl-L-homocysteine (L-SAH), with modifications in the amino acid or base portions of the molecule, have been synthesized and evaluated for their abilities to inhibit the transmethylations catalyzed by catechol O-methyltransferase (COMT), phenylethanolamine N-methyltransferase (PNMT), histamine N-methyltransferase (HMT), hydroxyindole O-methyltransferase (HIOMT), and indoleethylamine N-methyltransferase (INMT). From these studies some interesting and potentially useful differences in the structural features of L-SAH needed to produce maximal binding to these methyltransferases were detected. This paper provides evidence that 8-azaadenosyl-L-homocysteine is a potent and selective inhibitor of HIOMT, whereas N6-methyladenosyl-L-homocysteine and N6-methyl-3-deazaadenosyl-L-homocysteine are selective inhibitors in INMT. In contrast, it was found that S-tubercidinyl-L-homocysteine was a fairly potent, but nonselective inhibitor of all of the methyltransferases studied. The differences and the similarities in the requirements for the binding of SAH to methyltransferases which were detected in this study and earlier studies from our laboratory, are described. The possibilites of utilizing differences in binding requirements for the design of SAH analogues as specific inhibitors of methyltransferases are discussed.", "contents": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 4. Further modifications of the amino and base portions of S-adenosyl-L-homocysteine. Structural analogues of S-adenosyl-L-homocysteine (L-SAH), with modifications in the amino acid or base portions of the molecule, have been synthesized and evaluated for their abilities to inhibit the transmethylations catalyzed by catechol O-methyltransferase (COMT), phenylethanolamine N-methyltransferase (PNMT), histamine N-methyltransferase (HMT), hydroxyindole O-methyltransferase (HIOMT), and indoleethylamine N-methyltransferase (INMT). From these studies some interesting and potentially useful differences in the structural features of L-SAH needed to produce maximal binding to these methyltransferases were detected. This paper provides evidence that 8-azaadenosyl-L-homocysteine is a potent and selective inhibitor of HIOMT, whereas N6-methyladenosyl-L-homocysteine and N6-methyl-3-deazaadenosyl-L-homocysteine are selective inhibitors in INMT. In contrast, it was found that S-tubercidinyl-L-homocysteine was a fairly potent, but nonselective inhibitor of all of the methyltransferases studied. The differences and the similarities in the requirements for the binding of SAH to methyltransferases which were detected in this study and earlier studies from our laboratory, are described. The possibilites of utilizing differences in binding requirements for the design of SAH analogues as specific inhibitors of methyltransferases are discussed.", "PMID": 978673}
{"id": "pubmed23n0032_11829", "title": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 5. Role of the asymmetric sulfonium pole in the enzymatic binding of S-adenosyl-L-methionine.", "content": "The configuration at the asymmetric sulfonium pole of S-adenosyl-L-methionine (SAM) necessary for optimal enzymatic binding and methyl donation has been elucidated in this study. For the transmethylations catalyzed by catechol O-methyltransferase, phenylethanolamine N-methyltransferase, histamine N-methyltransferase, and hydroxyindole O-methyltransferase, it was demonstrated that only the natural (-) enantiomer of SAM was active as a methyl donor. The corresponding (+)-SAM, which was prepared by enzymatic resolution of synthetic (+/-)-SAM, was shown to be inactive as a methyl donor in these enzymatic reactions. The (+)-SAM was found, however, to be a potent inhibitor of each of these enzyme-catalyzed transmethylations. These results suggest that the (+) enantiomer offers a nonproductive configuration for the methyl-transfer reaction itself; however, this configuration fails to hamper enzymatic binding. These results are discussed relative to the geometric requirements necessary for the methyl-transfer reaction and the requirements for enzymatic binding.", "contents": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 5. Role of the asymmetric sulfonium pole in the enzymatic binding of S-adenosyl-L-methionine. The configuration at the asymmetric sulfonium pole of S-adenosyl-L-methionine (SAM) necessary for optimal enzymatic binding and methyl donation has been elucidated in this study. For the transmethylations catalyzed by catechol O-methyltransferase, phenylethanolamine N-methyltransferase, histamine N-methyltransferase, and hydroxyindole O-methyltransferase, it was demonstrated that only the natural (-) enantiomer of SAM was active as a methyl donor. The corresponding (+)-SAM, which was prepared by enzymatic resolution of synthetic (+/-)-SAM, was shown to be inactive as a methyl donor in these enzymatic reactions. The (+)-SAM was found, however, to be a potent inhibitor of each of these enzyme-catalyzed transmethylations. These results suggest that the (+) enantiomer offers a nonproductive configuration for the methyl-transfer reaction itself; however, this configuration fails to hamper enzymatic binding. These results are discussed relative to the geometric requirements necessary for the methyl-transfer reaction and the requirements for enzymatic binding.", "PMID": 978674}
{"id": "pubmed23n0032_11830", "title": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 6. Structural modifications of S-adenosylmethionine.", "content": "Structural analogues of S-adenosyl-L-methionine (SAM), with modifications in the amino acid, sugar, or base portions of the molecule, have been synthesized and evaluated as either inhibitors and/or substrates for the enzymes catechol O-methyltransferase, phenylethanolamine N-methyltransferase, histamine N-methyltransferase, and hydroxyindole O-methyltransferase. To evaluate these analogues as substrates for SAM-dependent methyltransferases, the corresponding methyl-14C compounds were prepared and tested for their abilities to donate their methyl group to the appropriate acceptor molecules. In addition, the unlabeled SAM analogues were tested for their inhibitory activities in these same transmethylation reactions. In general, it could be conlcuded from these studies that methyltransferases show very strict specificity for the structural features of SAM. This strict specificity holds for the enzymatic binding and methyl-donating abilities of this molecule. In fact, it could be concluded from the results of this study that methyltransferases show a higher specificity for the structural features of the substrate L-SAM than for the structural features of the product S-adenosyl-L-homocysteine (L-SAH).", "contents": "Potential inhibitors of S-adenosylmethionine-dependent methyltransferases. 6. Structural modifications of S-adenosylmethionine. Structural analogues of S-adenosyl-L-methionine (SAM), with modifications in the amino acid, sugar, or base portions of the molecule, have been synthesized and evaluated as either inhibitors and/or substrates for the enzymes catechol O-methyltransferase, phenylethanolamine N-methyltransferase, histamine N-methyltransferase, and hydroxyindole O-methyltransferase. To evaluate these analogues as substrates for SAM-dependent methyltransferases, the corresponding methyl-14C compounds were prepared and tested for their abilities to donate their methyl group to the appropriate acceptor molecules. In addition, the unlabeled SAM analogues were tested for their inhibitory activities in these same transmethylation reactions. In general, it could be conlcuded from these studies that methyltransferases show very strict specificity for the structural features of SAM. This strict specificity holds for the enzymatic binding and methyl-donating abilities of this molecule. In fact, it could be concluded from the results of this study that methyltransferases show a higher specificity for the structural features of the substrate L-SAM than for the structural features of the product S-adenosyl-L-homocysteine (L-SAH).", "PMID": 978675}
{"id": "pubmed23n0032_11831", "title": "Potential antitumor agents. 19. Multiply substituted 4'-(9-acridinylamino)methanesulfonanilides.", "content": "A series of 42 multiply substituted 4'-(9-acridinylamino)methanesulfonanilides has been prepared and evaluated in the L1210 system. In addition to biologic activity changes resulting from altered agent lipophilic-hydrophilic balance variants containing both acridine 4-CH3 and 3-NH2, NHCOCH3 or NO2 substituents have reduced activity. Variants 3,6-disubstituted, using functions of differing electronic character, have depressed activity, suggesting that there is limited site bulk tolerance. Asymmetric 3,5-disubstitution should then be the preferred pattern; the 3-ND-5CH-3'-OCH variant is the most dose potent (optimum dose qd 1-5, 1.25 mg/kg/day) of the high activity agents of this series so far prepared.", "contents": "Potential antitumor agents. 19. Multiply substituted 4'-(9-acridinylamino)methanesulfonanilides. A series of 42 multiply substituted 4'-(9-acridinylamino)methanesulfonanilides has been prepared and evaluated in the L1210 system. In addition to biologic activity changes resulting from altered agent lipophilic-hydrophilic balance variants containing both acridine 4-CH3 and 3-NH2, NHCOCH3 or NO2 substituents have reduced activity. Variants 3,6-disubstituted, using functions of differing electronic character, have depressed activity, suggesting that there is limited site bulk tolerance. Asymmetric 3,5-disubstitution should then be the preferred pattern; the 3-ND-5CH-3'-OCH variant is the most dose potent (optimum dose qd 1-5, 1.25 mg/kg/day) of the high activity agents of this series so far prepared.", "PMID": 978676}
{"id": "pubmed23n0032_11832", "title": "Structural requirements for biological activity among antileukemic glaucarubolone ester quassinoids.", "content": "A C-15 ester substituent is required for significant antileukemic activity among the glaucarubolone ester quassinoids, and variations in the ester group are not accompanied by particularly marked changes in antileukemic activity. Unsaturation at the 3,4 position is advantageous for optimal activity, and hydrogenation of this double bond results in marked diminution in both cytotoxicity toward KB cells in tissue culture and inhibitory activity against the P-388 lymphocytic leukemia in mice.", "contents": "Structural requirements for biological activity among antileukemic glaucarubolone ester quassinoids. A C-15 ester substituent is required for significant antileukemic activity among the glaucarubolone ester quassinoids, and variations in the ester group are not accompanied by particularly marked changes in antileukemic activity. Unsaturation at the 3,4 position is advantageous for optimal activity, and hydrogenation of this double bond results in marked diminution in both cytotoxicity toward KB cells in tissue culture and inhibitory activity against the P-388 lymphocytic leukemia in mice.", "PMID": 978677}
{"id": "pubmed23n0032_11833", "title": "Synthesis and antimyoctic properties of 1-(2-alkyl-2-phenylethyl)-1H-imidazoles.", "content": "The synthesis of 1-(2-alkyl-2-phenylethyl)-1H-imidazoles was accomplished starting from the corresponding phenylacetonitriles. Via alkylation, esterification, and sodium borohydride reduction-in the presence of lithium iodide-beta-phenylalconols were obtained. Mesylation of these alcohols and refluxing with imidazole in dimethylformamide furnished title compounds, which were active in vitro against dermatophytes, yeasts, other fungi, and gram-positive bacteria and in vivo as well as in vitro against Candida albicans.", "contents": "Synthesis and antimyoctic properties of 1-(2-alkyl-2-phenylethyl)-1H-imidazoles. The synthesis of 1-(2-alkyl-2-phenylethyl)-1H-imidazoles was accomplished starting from the corresponding phenylacetonitriles. Via alkylation, esterification, and sodium borohydride reduction-in the presence of lithium iodide-beta-phenylalconols were obtained. Mesylation of these alcohols and refluxing with imidazole in dimethylformamide furnished title compounds, which were active in vitro against dermatophytes, yeasts, other fungi, and gram-positive bacteria and in vivo as well as in vitro against Candida albicans.", "PMID": 978678}
{"id": "pubmed23n0032_11834", "title": "9-Nor-9-hydroxyhexahydrocannabinols. Synthesis, Some behavioral and analgesic properties, and comparison with the tetrahydrocannabinols.", "content": "The racemic mixture and levo isomer of both 9-nor-9alpha-hydroxyhexahydrocannabinol and its 9beta-hydroxy isomer were prepared. Both alpha-and beta-hydroxy compounds were active in the dog ataxia test and depressed spontaneous activity in mice. However, only the beta-hydroxy compound was an analgesic in mice morphine-like potency. The behavioral and analgesic properties of these compounds may be mediated through different sites or mechanisms and may, therefore, be separable.", "contents": "9-Nor-9-hydroxyhexahydrocannabinols. Synthesis, Some behavioral and analgesic properties, and comparison with the tetrahydrocannabinols. The racemic mixture and levo isomer of both 9-nor-9alpha-hydroxyhexahydrocannabinol and its 9beta-hydroxy isomer were prepared. Both alpha-and beta-hydroxy compounds were active in the dog ataxia test and depressed spontaneous activity in mice. However, only the beta-hydroxy compound was an analgesic in mice morphine-like potency. The behavioral and analgesic properties of these compounds may be mediated through different sites or mechanisms and may, therefore, be separable.", "PMID": 978681}
{"id": "pubmed23n0032_11835", "title": "Study of U.S. medical school applicants, 1974-75.", "content": "The applicant activity for the 1974-75 first-year class in U.S. medical schools presents an unusual combination of statistics. There were more applicants than ever before, but the annual growth rate showed a decline, a trend that began in 1973-74; the national acceptance average remained unchanged; and the average application frequency per individual was accelerated. Most of the traditional tables cover five years of historical data (1970-71 through 1974-75), while the new features--acceptance success for first-time and repeat applicants and ability levels by acceptance success and application frequency--depict data for one year (1974-75). As in past studies, the conclusion enumerates major efforts instituted by the Association of American Medical Colleges to assist medical schools with admission problems. The most important innovations among these were two new task forces--one for minority admissions and one for financial aid.", "contents": "Study of U.S. medical school applicants, 1974-75. The applicant activity for the 1974-75 first-year class in U.S. medical schools presents an unusual combination of statistics. There were more applicants than ever before, but the annual growth rate showed a decline, a trend that began in 1973-74; the national acceptance average remained unchanged; and the average application frequency per individual was accelerated. Most of the traditional tables cover five years of historical data (1970-71 through 1974-75), while the new features--acceptance success for first-time and repeat applicants and ability levels by acceptance success and application frequency--depict data for one year (1974-75). As in past studies, the conclusion enumerates major efforts instituted by the Association of American Medical Colleges to assist medical schools with admission problems. The most important innovations among these were two new task forces--one for minority admissions and one for financial aid.", "PMID": 978700}
{"id": "pubmed23n0032_11836", "title": "The future of pathology: a Delphi study by pathology department chairmen.", "content": "This paper reports the results of a study by and of chairmen of pathology departments in American and Canadian medical schools directed toward discovering chairmen's perceptions and attitudes about the future of pathology. Over 100 of a total of 143 chairmen participated in some or all phases of this study in which the Delphi approach was used. Sixty-five chairmen completed all four rounds of questionnaires. Most chairmen are deeply concerned about increasing federal regulation of medicine. They expect to play a greater role in all phases of medical education with more emphasis on the basic science aspects of pathology. They also foresee a desirable shifting of residency training programs away from small hospitals to the larger medical centers. This shift will result in fewer but longer training programs.", "contents": "The future of pathology: a Delphi study by pathology department chairmen. This paper reports the results of a study by and of chairmen of pathology departments in American and Canadian medical schools directed toward discovering chairmen's perceptions and attitudes about the future of pathology. Over 100 of a total of 143 chairmen participated in some or all phases of this study in which the Delphi approach was used. Sixty-five chairmen completed all four rounds of questionnaires. Most chairmen are deeply concerned about increasing federal regulation of medicine. They expect to play a greater role in all phases of medical education with more emphasis on the basic science aspects of pathology. They also foresee a desirable shifting of residency training programs away from small hospitals to the larger medical centers. This shift will result in fewer but longer training programs.", "PMID": 978701}
{"id": "pubmed23n0032_11837", "title": "A standardized interviewing technique for evaluating postgraduate training applicants.", "content": "In an effort to streamline the personal interview and to reduce the influence of subjectivity inpreparation of ranking lists for postgraduate year-one applicants, a standardized interview was constructed for use in evaluating condidates for admission to a training program in internal medicine. The interview was conducted in a uniformly structured manner by 14 interviewers of 260 applicants. Each applicant received an interview score which correlated well with the final decision of the Intern Selection Committee. For each interviewer various measures of performance were obtained which allowed a classification into \"hard\" versus \"easy\" interviewer and indicated the comparability of each interview's evaluations with the final decisions. Thus, the standardized interview resulted in quantifiable data in the form of uniform evaluations of applicants and interviewers.", "contents": "A standardized interviewing technique for evaluating postgraduate training applicants. In an effort to streamline the personal interview and to reduce the influence of subjectivity inpreparation of ranking lists for postgraduate year-one applicants, a standardized interview was constructed for use in evaluating condidates for admission to a training program in internal medicine. The interview was conducted in a uniformly structured manner by 14 interviewers of 260 applicants. Each applicant received an interview score which correlated well with the final decision of the Intern Selection Committee. For each interviewer various measures of performance were obtained which allowed a classification into \"hard\" versus \"easy\" interviewer and indicated the comparability of each interview's evaluations with the final decisions. Thus, the standardized interview resulted in quantifiable data in the form of uniform evaluations of applicants and interviewers.", "PMID": 978702}
{"id": "pubmed23n0032_11838", "title": "A study of student attitudes toward a psychiatric clerkship.", "content": "Sixty-seven third-year medical students at the University of Ottawa were interviewed at the beginning and end of a month-long clerkship in psychiatry. Two attitude categories were designated: \"attitude-positive\" (AP) differentiated subjects with positive clerkship expectations or attitudes for \"attitude-negative\" (AN) subjects who had negative expectations or attitudes. Differences between preclerkship APs and ANs are described. Postclerkship interviews revealed a general improvement in attitudes toward psychiatry and the psychiatric clerkship. Statistically significant differences were noted between postclerkship APs and ANs in the following: attitudes toward psychiatry, attainment of preclerkship objectives, reduction of anxiety levels, and nature of supervisory experiences. Postclerkship attitudes may be predicted to some extent prior to the clerkship. The potential for a negative postclerkship attitude may be modified by positive clerkship experiences. The importance of identifying students vulnerable to the stresses of psychiatric clerkship is emphasized.", "contents": "A study of student attitudes toward a psychiatric clerkship. Sixty-seven third-year medical students at the University of Ottawa were interviewed at the beginning and end of a month-long clerkship in psychiatry. Two attitude categories were designated: \"attitude-positive\" (AP) differentiated subjects with positive clerkship expectations or attitudes for \"attitude-negative\" (AN) subjects who had negative expectations or attitudes. Differences between preclerkship APs and ANs are described. Postclerkship interviews revealed a general improvement in attitudes toward psychiatry and the psychiatric clerkship. Statistically significant differences were noted between postclerkship APs and ANs in the following: attitudes toward psychiatry, attainment of preclerkship objectives, reduction of anxiety levels, and nature of supervisory experiences. Postclerkship attitudes may be predicted to some extent prior to the clerkship. The potential for a negative postclerkship attitude may be modified by positive clerkship experiences. The importance of identifying students vulnerable to the stresses of psychiatric clerkship is emphasized.", "PMID": 978703}
{"id": "pubmed23n0032_11839", "title": "Pediatric-psychiatric liaison as a model for teaching pediatric residents.", "content": "The pediatrician's function as a comprehensive family health practitioner has frequently been ignored. A major problem is that pediatric residents are not trained or are inadequately trained to assess emotional, behavioral, and family problems that may be an integral part of the patient's symptoms. At the Children's Hospital of Philadelphia, a pediatric-psychiatric liaison program has evolved in which pediatric residents learn the principles and skills necessary to manage these problems. Ultimately, the liaison program will enable the staff pediatricians who use these principles to assume appropriate responsibility for educating the pediatric trainees.", "contents": "Pediatric-psychiatric liaison as a model for teaching pediatric residents. The pediatrician's function as a comprehensive family health practitioner has frequently been ignored. A major problem is that pediatric residents are not trained or are inadequately trained to assess emotional, behavioral, and family problems that may be an integral part of the patient's symptoms. At the Children's Hospital of Philadelphia, a pediatric-psychiatric liaison program has evolved in which pediatric residents learn the principles and skills necessary to manage these problems. Ultimately, the liaison program will enable the staff pediatricians who use these principles to assume appropriate responsibility for educating the pediatric trainees.", "PMID": 978704}
{"id": "pubmed23n0032_11840", "title": "Electrolyte transport by gallbladders of rabbit and guinea pig: effect of amphotericin B and evidence of rheogenic Na transport.", "content": "Ion transport and electrical properties of rabbit and guinea pig gallbladders were investigated to gain further information about the active transport mechanism that mediates fluid absorption. The intracellular and transepithelial electrical potentials were measured simultaneously using the microelectrode technique. Exposure of the mucosal surface to Amphotericin B resulted in the prompt development of a serosa-positive electrical potential difference (PD) which could not be attributed to an alteration in ion diffusion potentials across either the cell membrane or across the tight junction. Because the Amphotericin B-induced PD was immediately dependent on warm temperatures and O2, and was independent of NA and K concentration gradients across the cell membrane, it is suggested that active ion transport is directly responsible for the PD. Since the PD was abolished in the absence of Na in the bathing solutions, a rheogenic Na pump is postulated; this pump also appears to be operative in tissues not exposed to Amphotericin B. The specific tissue properties altered by Amphotericin B to produce a serosa-positive PD remain incompletely defined. The results of the present study indicate that ion transport by rabbit gallbladder in vitro is a consequence of a rheogenic active Na transport mechanism at the basolateral membranes which, in conjunction with a coupled NaC1 influx process at the mucosal border, ultimately results in absorption of NaC1 and water.", "contents": "Electrolyte transport by gallbladders of rabbit and guinea pig: effect of amphotericin B and evidence of rheogenic Na transport. Ion transport and electrical properties of rabbit and guinea pig gallbladders were investigated to gain further information about the active transport mechanism that mediates fluid absorption. The intracellular and transepithelial electrical potentials were measured simultaneously using the microelectrode technique. Exposure of the mucosal surface to Amphotericin B resulted in the prompt development of a serosa-positive electrical potential difference (PD) which could not be attributed to an alteration in ion diffusion potentials across either the cell membrane or across the tight junction. Because the Amphotericin B-induced PD was immediately dependent on warm temperatures and O2, and was independent of NA and K concentration gradients across the cell membrane, it is suggested that active ion transport is directly responsible for the PD. Since the PD was abolished in the absence of Na in the bathing solutions, a rheogenic Na pump is postulated; this pump also appears to be operative in tissues not exposed to Amphotericin B. The specific tissue properties altered by Amphotericin B to produce a serosa-positive PD remain incompletely defined. The results of the present study indicate that ion transport by rabbit gallbladder in vitro is a consequence of a rheogenic active Na transport mechanism at the basolateral membranes which, in conjunction with a coupled NaC1 influx process at the mucosal border, ultimately results in absorption of NaC1 and water.", "PMID": 978715}
{"id": "pubmed23n0032_11841", "title": "A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS).", "content": "DIDS (4,4'-diisothiocyano stilbene-2,2'-disulfonic acid) and H2DIDS (4,4'-diisothiocyano-1,2-diphenyl ethane-2,2'-disulfonic acid) binding to the human red cell membrane proteins were studied as a function of concentration, temperature and time. Most binding sites were common to both. The common sites were in band 3 of SDS polyacrylamide gel electropherograms (Steck, 1974. J. Cell Biol. 62:1), an unidentified adjacent band, and glycophorin. Reversible and irreversible binding occurred; both inhibited sulfate equilibrium exchange. The time courses of irreversible binding to band 3 and total binding to the membrane as a whole were biphasic. About 20% of H2DIDS and greater 60% of DIDS binding were rapid, independent of temperature. Slow H2-DIDS binding was monoexponential, activation enthalpy 23 kcal/mole. The stoichiometry of irreversible H2DIDS binding to band 3 was 1.1-1.2, concentration-dependent. Under the conditions studied (0-50 muM, hematocrit 10%, 5-37 degrees C) binding to band 3 was a constant fraction of total binding, 0.7 for H2DIDS and 0.8 for DIDS. Inhibition was a linear function of total binding, binding to band 3, and therefore also to nonband 3 sites, with either inhibitor during both phases, H2DIDS inhibition was complete at 1.9 X 10(6) or 1.2 X 10(6) molecules/cell total and band 3 binding respectively. For DIDS the corresponding figures were 1.3 X 10(6) and 1.1 X 10(6). It is shown how reagents of mixed function can react with biphasic kinetics. Binding to multiple contiguous sites may exhibit concentration-dependent stoichiometry. Under such conditions a linear inhibition-binding relationship is neither a necessary nor a sufficient condition for the identification of transport sites.", "contents": "A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS). DIDS (4,4'-diisothiocyano stilbene-2,2'-disulfonic acid) and H2DIDS (4,4'-diisothiocyano-1,2-diphenyl ethane-2,2'-disulfonic acid) binding to the human red cell membrane proteins were studied as a function of concentration, temperature and time. Most binding sites were common to both. The common sites were in band 3 of SDS polyacrylamide gel electropherograms (Steck, 1974. J. Cell Biol. 62:1), an unidentified adjacent band, and glycophorin. Reversible and irreversible binding occurred; both inhibited sulfate equilibrium exchange. The time courses of irreversible binding to band 3 and total binding to the membrane as a whole were biphasic. About 20% of H2DIDS and greater 60% of DIDS binding were rapid, independent of temperature. Slow H2-DIDS binding was monoexponential, activation enthalpy 23 kcal/mole. The stoichiometry of irreversible H2DIDS binding to band 3 was 1.1-1.2, concentration-dependent. Under the conditions studied (0-50 muM, hematocrit 10%, 5-37 degrees C) binding to band 3 was a constant fraction of total binding, 0.7 for H2DIDS and 0.8 for DIDS. Inhibition was a linear function of total binding, binding to band 3, and therefore also to nonband 3 sites, with either inhibitor during both phases, H2DIDS inhibition was complete at 1.9 X 10(6) or 1.2 X 10(6) molecules/cell total and band 3 binding respectively. For DIDS the corresponding figures were 1.3 X 10(6) and 1.1 X 10(6). It is shown how reagents of mixed function can react with biphasic kinetics. Binding to multiple contiguous sites may exhibit concentration-dependent stoichiometry. Under such conditions a linear inhibition-binding relationship is neither a necessary nor a sufficient condition for the identification of transport sites.", "PMID": 978716}
{"id": "pubmed23n0032_11842", "title": "On the question of an electrokinetic requirement for phospholipase C action.", "content": "The phospholipase C of clostridium welchii (alpha toxin) has an absolute requirement for trace quantities of Ca2+. It attacks pure phosphatidylcholine particles (smectic mesophases) having a close-packed bilayer structure only when their surface zeta potential is made positive by the addition of certain divalent ions (e.g., Ca2+) to the aqueous phase or by the presence of low concentrations of long chain cations to the lipid. Alternatively, if the rotational freedom of individual phospholipid molecules is increased by the insertion of short n-alkanols (e.g., hexanol) into the bilayer or when a monolayer of the substrate at an air/water interface is expended, enzymic hydrolysis can occur without any requirement for a net postive charge on the surface.", "contents": "On the question of an electrokinetic requirement for phospholipase C action. The phospholipase C of clostridium welchii (alpha toxin) has an absolute requirement for trace quantities of Ca2+. It attacks pure phosphatidylcholine particles (smectic mesophases) having a close-packed bilayer structure only when their surface zeta potential is made positive by the addition of certain divalent ions (e.g., Ca2+) to the aqueous phase or by the presence of low concentrations of long chain cations to the lipid. Alternatively, if the rotational freedom of individual phospholipid molecules is increased by the insertion of short n-alkanols (e.g., hexanol) into the bilayer or when a monolayer of the substrate at an air/water interface is expended, enzymic hydrolysis can occur without any requirement for a net postive charge on the surface.", "PMID": 978717}
{"id": "pubmed23n0032_11843", "title": "\"Metabolic\" action potentials in Acetabularia.", "content": "The transient depolarizations in Acetabularia which fulfill the essential criteria of an action potential (all-or-none characteristics, triggering by depolarization, propagation, etc.) are investigated. These action potentials are analyzed by conductance measurements and voltage clamp experiments on the basis of the analog circuit of the membrane (Gradmann, D. 1975, J. Membrane Biol. 25:183). It is concluded that these action potentials do not arise by permeability changes of the passive diffusion channels, but by the active pathway of the electrogenic pump, which consists of a voltage source EP of about --20 mV in series with two nonlinear conducting elements P1 and P2, the latter and EP being shunted by a large quasi capacity CP of some mF cm-2. The nonlinear current-voltage relationship of the carrier system (P1) is not changed during the action potential but has an effect on its time course. However, the elements P2 and CP, which probably reflect metabolic entities, are suggested to control the action potentials.", "contents": "\"Metabolic\" action potentials in Acetabularia. The transient depolarizations in Acetabularia which fulfill the essential criteria of an action potential (all-or-none characteristics, triggering by depolarization, propagation, etc.) are investigated. These action potentials are analyzed by conductance measurements and voltage clamp experiments on the basis of the analog circuit of the membrane (Gradmann, D. 1975, J. Membrane Biol. 25:183). It is concluded that these action potentials do not arise by permeability changes of the passive diffusion channels, but by the active pathway of the electrogenic pump, which consists of a voltage source EP of about --20 mV in series with two nonlinear conducting elements P1 and P2, the latter and EP being shunted by a large quasi capacity CP of some mF cm-2. The nonlinear current-voltage relationship of the carrier system (P1) is not changed during the action potential but has an effect on its time course. However, the elements P2 and CP, which probably reflect metabolic entities, are suggested to control the action potentials.", "PMID": 978718}
{"id": "pubmed23n0032_11844", "title": "Electrical properties of Valonia ventricosa.", "content": "The cytoplasmic electrical potential and membrane resistance of mature cells of Valonia ventricosa have been measured by inserting a microelectrode concentric with another electrode into the vacuole of the cell. The cytoplasmic region was investigated by advancing the microelectrode into the cell wall from the vascuolar side. The results revealed a unique region where the vascuolar electric potential and membrane resistance changed in a simultaneous single step to values close to zero. The measured potential always remained positive immediately after the step. At no time was a highly negative potential region encountered. Further penetration of the microelectrode revealed a low resistance negative potential region of --12.6 +/- 1.1 mV associated with the cell wall. Experiments were also carried out on aplanospores of V. ventricosa to compare mature and immature cells. The chemical composition of the vacuolar and protoplasmic phases of mature cells was determined. The results agreed with previous results except that the C1- ion content of the protoplasm was significantly higher at 381 +/- 20 mmoles/liter (H2O). It was concluded that mature cells of Valonia are significantly different from immature cells in that no highly negative potential cytoplasmic region was found in mature cells. It was considered that the measured step change in electric potential and membrane resistance occurred at the plasmalemma and that the tonoplast was a region of very low resistance. The implications of these findings in terms of models of ion transport into Valonia are discussed.", "contents": "Electrical properties of Valonia ventricosa. The cytoplasmic electrical potential and membrane resistance of mature cells of Valonia ventricosa have been measured by inserting a microelectrode concentric with another electrode into the vacuole of the cell. The cytoplasmic region was investigated by advancing the microelectrode into the cell wall from the vascuolar side. The results revealed a unique region where the vascuolar electric potential and membrane resistance changed in a simultaneous single step to values close to zero. The measured potential always remained positive immediately after the step. At no time was a highly negative potential region encountered. Further penetration of the microelectrode revealed a low resistance negative potential region of --12.6 +/- 1.1 mV associated with the cell wall. Experiments were also carried out on aplanospores of V. ventricosa to compare mature and immature cells. The chemical composition of the vacuolar and protoplasmic phases of mature cells was determined. The results agreed with previous results except that the C1- ion content of the protoplasm was significantly higher at 381 +/- 20 mmoles/liter (H2O). It was concluded that mature cells of Valonia are significantly different from immature cells in that no highly negative potential cytoplasmic region was found in mature cells. It was considered that the measured step change in electric potential and membrane resistance occurred at the plasmalemma and that the tonoplast was a region of very low resistance. The implications of these findings in terms of models of ion transport into Valonia are discussed.", "PMID": 978719}
{"id": "pubmed23n0032_11845", "title": "Early chick neuroretinal responses following direct exposure to methotrexate.", "content": "Explants of 4.5-day-old chick embryonic neuroretinas with mesenchyme were exposed to Methotrexate (MTX) in medium 199 with embryo extract. Proliferative responses of the cultured neuroretinas were followed radioautographically by administration of 3H-thymidine to the cultures. The DNA synthetic, mitotic and pyknotic responses of the ventricular cells of the neuroretina were followed over a 16-hour period. The responses observed suggested that MTX caused a synchronization of the ventricular cells in the pre-mitotic phases with no direct inhibition of mitosis. Furthermore, prolonged exposure to MTX resulted in the accumulation of labeled pyknotic cells, indicating a decline in the regenerative capacity of the proliferative ventricular cells.", "contents": "Early chick neuroretinal responses following direct exposure to methotrexate. Explants of 4.5-day-old chick embryonic neuroretinas with mesenchyme were exposed to Methotrexate (MTX) in medium 199 with embryo extract. Proliferative responses of the cultured neuroretinas were followed radioautographically by administration of 3H-thymidine to the cultures. The DNA synthetic, mitotic and pyknotic responses of the ventricular cells of the neuroretina were followed over a 16-hour period. The responses observed suggested that MTX caused a synchronization of the ventricular cells in the pre-mitotic phases with no direct inhibition of mitosis. Furthermore, prolonged exposure to MTX resulted in the accumulation of labeled pyknotic cells, indicating a decline in the regenerative capacity of the proliferative ventricular cells.", "PMID": 978746}
{"id": "pubmed23n0032_11846", "title": "A stereological analysis of the ultrastructure of the lungs of wild mice living at low and high altitude.", "content": "Stereological analysis of the ultrastructural composition of the pulmonary alveolo-capillary region of mice living at sea level compared with that of the same species (Phyllotis darwini) genetically adapted to life at 4,660 m reveals a trend at high altitude towards a greater volume percentage of tissue components. On a weight-specific basis, non-circulating tissue occupies a significantly greater volume in high-altitude mice, but air space and capillary contents are not correspondingly greater. Since the arithmetic mean thickness of the tissue layers and of the air-blood barrier are the same in the two altitudinal groups, the average alveolus must have a smaller volume in the high-altitude mice. Epithelial, endothelial, and erythrocyte surface areas per gram body weight are significantly greater in the high-altitude mice. Nuclear counts indicate that the larger lungs of mice adapted to high altitude are due to larger Pneumocyte I and II and endothelial cells rather than to an increase in the number of these cells. Hematocrits measured within the pulmonary capillaries in the two altitudinal groups were equal. An heretofore unrecognized feature of possible adaptive value is the surface/volume ratio of erythrocytes, which is similar for erythrocytes in alveolar space of mice at low and high altitudes but within lung capillaries is 14.7% greater at high altitude.", "contents": "A stereological analysis of the ultrastructure of the lungs of wild mice living at low and high altitude. Stereological analysis of the ultrastructural composition of the pulmonary alveolo-capillary region of mice living at sea level compared with that of the same species (Phyllotis darwini) genetically adapted to life at 4,660 m reveals a trend at high altitude towards a greater volume percentage of tissue components. On a weight-specific basis, non-circulating tissue occupies a significantly greater volume in high-altitude mice, but air space and capillary contents are not correspondingly greater. Since the arithmetic mean thickness of the tissue layers and of the air-blood barrier are the same in the two altitudinal groups, the average alveolus must have a smaller volume in the high-altitude mice. Epithelial, endothelial, and erythrocyte surface areas per gram body weight are significantly greater in the high-altitude mice. Nuclear counts indicate that the larger lungs of mice adapted to high altitude are due to larger Pneumocyte I and II and endothelial cells rather than to an increase in the number of these cells. Hematocrits measured within the pulmonary capillaries in the two altitudinal groups were equal. An heretofore unrecognized feature of possible adaptive value is the surface/volume ratio of erythrocytes, which is similar for erythrocytes in alveolar space of mice at low and high altitudes but within lung capillaries is 14.7% greater at high altitude.", "PMID": 978747}
{"id": "pubmed23n0032_11847", "title": "Formation of nucleoside 5'-polyphosphates under potentially prebiological conditions.", "content": "Aqueous solutions of linear inorganic polyphosphates incubated in presence of Mg ions depolymerize to give trimetaphosphate. The presence of a nucleoside 5'-phosphate has little influence upon the reaction. Drying the products obtained by incubating a linear polyphosphate with Mg ions in the presence of a nucleoside 5'-phosphate yields nucleoside 5'-polyphosphates. The prebiological relevance of the reactions is discussed.", "contents": "Formation of nucleoside 5'-polyphosphates under potentially prebiological conditions. Aqueous solutions of linear inorganic polyphosphates incubated in presence of Mg ions depolymerize to give trimetaphosphate. The presence of a nucleoside 5'-phosphate has little influence upon the reaction. Drying the products obtained by incubating a linear polyphosphate with Mg ions in the presence of a nucleoside 5'-phosphate yields nucleoside 5'-polyphosphates. The prebiological relevance of the reactions is discussed.", "PMID": 978748}
{"id": "pubmed23n0032_11848", "title": "Penguin evolution: protein comparisons demonstrate phylogenetic relationship to flying aquatic birds.", "content": "Quantitative immunological comparisons of three avian proteins, transferrin, ovalbumin, and penalbumin, indicate that penguins are phylogenetically most closely related to loons, albatrosses, herons, and grebes. These data support the theory that the ancestors of penguins were flying oceanic birds and that flightlessness in penguins has evolved independently from flightlessness in ratites.", "contents": "Penguin evolution: protein comparisons demonstrate phylogenetic relationship to flying aquatic birds. Quantitative immunological comparisons of three avian proteins, transferrin, ovalbumin, and penalbumin, indicate that penguins are phylogenetically most closely related to loons, albatrosses, herons, and grebes. These data support the theory that the ancestors of penguins were flying oceanic birds and that flightlessness in penguins has evolved independently from flightlessness in ratites.", "PMID": 978750}
{"id": "pubmed23n0032_11849", "title": "Human skeletal muscle proteins. The primary structure of troponin C.", "content": "The primary structure of the major component of human skeletal muscle troponin C has been established. The troponin C was purified by ammonium sulphate and isoelectric fractionation, followed by two chromatographic steps on DEAE Sephadex. The sequence was determined from the different overlapping enzymic peptides and by dansyl-Edman degradation. The only difference between rabbit skeletal muscle troponin C and the major component of human skeletal troponin C was found at position 112: Ala (rabbit), Pro (human). The partial amino acid sequence of the first 86 residues of the minor component of human skeletal troponin C was found to resemble the troponin C from bovine cardiac muscle. The only difference between them, has tentatively been located at position 62: Glu (human), Asp (bovine). These similarities suggest that troponin C is, from the point of view of molecular, one of the most conservative proteins so far studied.", "contents": "Human skeletal muscle proteins. The primary structure of troponin C. The primary structure of the major component of human skeletal muscle troponin C has been established. The troponin C was purified by ammonium sulphate and isoelectric fractionation, followed by two chromatographic steps on DEAE Sephadex. The sequence was determined from the different overlapping enzymic peptides and by dansyl-Edman degradation. The only difference between rabbit skeletal muscle troponin C and the major component of human skeletal troponin C was found at position 112: Ala (rabbit), Pro (human). The partial amino acid sequence of the first 86 residues of the minor component of human skeletal troponin C was found to resemble the troponin C from bovine cardiac muscle. The only difference between them, has tentatively been located at position 62: Glu (human), Asp (bovine). These similarities suggest that troponin C is, from the point of view of molecular, one of the most conservative proteins so far studied.", "PMID": 978749}
{"id": "pubmed23n0032_11850", "title": "Evolution of flightless land birds on southern continents: transferrin comparison shows monophyletic origin of ratites.", "content": "A biochemical approach was used to study the evolution of ratite birds, i.e., the ostriches, rheas, cassowaries, emus, and kiwis. Quantitative immunological comparison of transferrin from ratites, tinamous, and other flying birds indicates that all the ratites and tinamous are allied phylogenetically and that they are of monophyletic origin relative to other birds. To explain the current geographic distribution of ratites and the magnitude of the transferrin distances, it is supposed that the ancestors of these flightless birds walked across land bridges between the southern continents during Cretaceous times.", "contents": "Evolution of flightless land birds on southern continents: transferrin comparison shows monophyletic origin of ratites. A biochemical approach was used to study the evolution of ratite birds, i.e., the ostriches, rheas, cassowaries, emus, and kiwis. Quantitative immunological comparison of transferrin from ratites, tinamous, and other flying birds indicates that all the ratites and tinamous are allied phylogenetically and that they are of monophyletic origin relative to other birds. To explain the current geographic distribution of ratites and the magnitude of the transferrin distances, it is supposed that the ancestors of these flightless birds walked across land bridges between the southern continents during Cretaceous times.", "PMID": 978751}
{"id": "pubmed23n0032_11851", "title": "A preliminary classification of human functional sexual disorders.", "content": "A preliminary classification is presented for functional human sexual disorders. This system is based on objective behavior and reports of distress. Five categories of sexual disorders are proposed, including the behavioral, psychological and informational components of sexual functioning in the individual and the couple.", "contents": "A preliminary classification of human functional sexual disorders. A preliminary classification is presented for functional human sexual disorders. This system is based on objective behavior and reports of distress. Five categories of sexual disorders are proposed, including the behavioral, psychological and informational components of sexual functioning in the individual and the couple.", "PMID": 978754}
{"id": "pubmed23n0032_11852", "title": "Primary orgasmic dysfunction: essential treatment components.", "content": "In an attempt to determine the essential components of a successful treatment regime for primary orgasmic dysfunction, the principle treatment approaches are reviewed to elucidate the similarities in different programs which have reported similar outcome data. It is concluded that the systematic desensitization studies, the masturbatory training approaches, and the Reproductive Biology Research Foundation series are all effective treatment procedures. It appears that all three approaches employ graduated exposure to sexual stimuli plus some form of directive psychotherapy emphasizing interpersonal skills acquisition. These may be the essential treatment components.", "contents": "Primary orgasmic dysfunction: essential treatment components. In an attempt to determine the essential components of a successful treatment regime for primary orgasmic dysfunction, the principle treatment approaches are reviewed to elucidate the similarities in different programs which have reported similar outcome data. It is concluded that the systematic desensitization studies, the masturbatory training approaches, and the Reproductive Biology Research Foundation series are all effective treatment procedures. It appears that all three approaches employ graduated exposure to sexual stimuli plus some form of directive psychotherapy emphasizing interpersonal skills acquisition. These may be the essential treatment components.", "PMID": 978755}
{"id": "pubmed23n0032_11853", "title": "A short-term, intensive workshop approach for the treatment of human sexual inadequacy.", "content": "A rapid treatment approach to common sexual dysfunctions has been used in treating 59 couples, including 74 symptomatic individuals. Using a multidisciplinary team approach in an intensive 2 1/2 day workshop format, symptom improvement has been obtained in 86.5% of the dysfunctional individuals, with complete symptom reversal in 63.5%. The intensive workshop format has resulted in a saving of both patient and professional time and is seen as a viable and successful alternative to more time-consuming approaches of treating various sexual dysfunctions.", "contents": "A short-term, intensive workshop approach for the treatment of human sexual inadequacy. A rapid treatment approach to common sexual dysfunctions has been used in treating 59 couples, including 74 symptomatic individuals. Using a multidisciplinary team approach in an intensive 2 1/2 day workshop format, symptom improvement has been obtained in 86.5% of the dysfunctional individuals, with complete symptom reversal in 63.5%. The intensive workshop format has resulted in a saving of both patient and professional time and is seen as a viable and successful alternative to more time-consuming approaches of treating various sexual dysfunctions.", "PMID": 978756}
{"id": "pubmed23n0032_11854", "title": "Perceptual accuracy as a variable in marital adjustment.", "content": "This study investigated the ability of maritally-adjusted couples, couples attaining a divorce, and couples in counseling to predict the rewarding effects of their behavior on their spouse. Twenty-six couples, five obtaining a divorce, nine in counseling, and twelve adjusted couples completed a Marital Interaction Questionnaire in which they rated how rewarding each of the behaviors depicted was to them. Then each member predicted how rewarding it was to their spouse. Behaviors depicting six areas of marital interaction were included in the questionnaire. The computed perceptual accuracy scores were analyzed by a three-way classification-Analysis of Variance (ANOVA)-for unequal n's. This analysis yielded a significant main effect of marital status and a significant interaction between marital status by sex of spouse and between marital status by area of interaction. The maritally-adjusted group was always more accurate than the other two groups in their predictions, however, they were significantly more accurate only for selected areas of interaction.", "contents": "Perceptual accuracy as a variable in marital adjustment. This study investigated the ability of maritally-adjusted couples, couples attaining a divorce, and couples in counseling to predict the rewarding effects of their behavior on their spouse. Twenty-six couples, five obtaining a divorce, nine in counseling, and twelve adjusted couples completed a Marital Interaction Questionnaire in which they rated how rewarding each of the behaviors depicted was to them. Then each member predicted how rewarding it was to their spouse. Behaviors depicting six areas of marital interaction were included in the questionnaire. The computed perceptual accuracy scores were analyzed by a three-way classification-Analysis of Variance (ANOVA)-for unequal n's. This analysis yielded a significant main effect of marital status and a significant interaction between marital status by sex of spouse and between marital status by area of interaction. The maritally-adjusted group was always more accurate than the other two groups in their predictions, however, they were significantly more accurate only for selected areas of interaction.", "PMID": 978757}
{"id": "pubmed23n0032_11855", "title": "In every marriage there are two marriages.", "content": "While investigators often have commented about discrepancies in the way a husband and a wife view the same marriage, little attention has been devoted to the implications such discrepancies have for marital and family therapy. As part of a continuing study of the differences between couples who seek marital therapy and those who seek sexual dysfunction therapy, this report examines these two clinical populations on the dimension of discrepant views of the marriage. Our results suggest that overall discrepancy scores may be an important indicator of stress between partners. In particular areas, however, such as rating the quality of the marriage, specific complaints about the marriage, and concerns about male sexual difficulties, marital therapy patients obtained significantly higher discrepancy scores than sexual therapy patients.", "contents": "In every marriage there are two marriages. While investigators often have commented about discrepancies in the way a husband and a wife view the same marriage, little attention has been devoted to the implications such discrepancies have for marital and family therapy. As part of a continuing study of the differences between couples who seek marital therapy and those who seek sexual dysfunction therapy, this report examines these two clinical populations on the dimension of discrepant views of the marriage. Our results suggest that overall discrepancy scores may be an important indicator of stress between partners. In particular areas, however, such as rating the quality of the marriage, specific complaints about the marriage, and concerns about male sexual difficulties, marital therapy patients obtained significantly higher discrepancy scores than sexual therapy patients.", "PMID": 978758}
{"id": "pubmed23n0032_11856", "title": "Gender and sexual experience as determinants in a sexual behavior hierarchy.", "content": "The present study sought to assess the influence of gender and previous sexual experience regarding the structure of the basic sexual behavior hierarchy. Results indicated that both gender and previous experience have significant effects. Overall, gender appeared to have a less substantial effect than previous experience, being more noteworthy for the similarities manifest between males and females than the few differences observed. Previous experience displayed a substantial effect, particularly in the upper portion of the hierarchy, with like-experienced groups showing marked similarity in their hierarchy patterns. Several significant gender-experience interactions were found regarding oral-genital behaviors, and a general trend was observed for females indicating negative appreciation from pre to postexperience. Males revealed positive appreciation from pre to postexperience on all but the most basic sexual behaviors. Conclusions were to the effect that while both variables influenced the hierarchy, effects were complex, and often mediated by additional factors.", "contents": "Gender and sexual experience as determinants in a sexual behavior hierarchy. The present study sought to assess the influence of gender and previous sexual experience regarding the structure of the basic sexual behavior hierarchy. Results indicated that both gender and previous experience have significant effects. Overall, gender appeared to have a less substantial effect than previous experience, being more noteworthy for the similarities manifest between males and females than the few differences observed. Previous experience displayed a substantial effect, particularly in the upper portion of the hierarchy, with like-experienced groups showing marked similarity in their hierarchy patterns. Several significant gender-experience interactions were found regarding oral-genital behaviors, and a general trend was observed for females indicating negative appreciation from pre to postexperience. Males revealed positive appreciation from pre to postexperience on all but the most basic sexual behaviors. Conclusions were to the effect that while both variables influenced the hierarchy, effects were complex, and often mediated by additional factors.", "PMID": 978760}
{"id": "pubmed23n0032_11857", "title": "Complement-fixing antigen of human sarcomas.", "content": "Further investigation of the humoral immune responses of patients with sarcoma to their tumors revealed a sarcoma-associated antigen that was readily detected by complement fixation. Circulating levels of antibody to this antigen, tentatively labeled S3, rose markedly after surgical removal of the tumor. Antibody to S3, as to S1 and S2, was highly prevalent in patients with various malignant tumors other than sarcoma.", "contents": "Complement-fixing antigen of human sarcomas. Further investigation of the humoral immune responses of patients with sarcoma to their tumors revealed a sarcoma-associated antigen that was readily detected by complement fixation. Circulating levels of antibody to this antigen, tentatively labeled S3, rose markedly after surgical removal of the tumor. Antibody to S3, as to S1 and S2, was highly prevalent in patients with various malignant tumors other than sarcoma.", "PMID": 978761}
{"id": "pubmed23n0032_11858", "title": "Epidemiology of renal cell carcinoma in Connecticut, 1935-1973.", "content": "Data on over 3,700 patients with renal cell carcinoma, reported to the Connecticut Tumor Registry from 1935 through 1973, were used to assess incidence, survival, and associations of risk with demographic characteristics. Incidence increased over time among men, but not among women; a birth cohort effect suggesting increasing incidence rate over time was demonstrated for men. A comparison of male and female age-specific incidence rates indicated that, in the 15- to 39-year-old age group, men were three times more likely than women to develop the disease; after age 40, renal cell carcinoma was diagnosed in men twice as often as in women. Survival probability has increased from 1940 to the present time. A high density of persons per square mile was associated with a higher-than-expected incidence. No trends in incidence according to socioeconomic status were observed.", "contents": "Epidemiology of renal cell carcinoma in Connecticut, 1935-1973. Data on over 3,700 patients with renal cell carcinoma, reported to the Connecticut Tumor Registry from 1935 through 1973, were used to assess incidence, survival, and associations of risk with demographic characteristics. Incidence increased over time among men, but not among women; a birth cohort effect suggesting increasing incidence rate over time was demonstrated for men. A comparison of male and female age-specific incidence rates indicated that, in the 15- to 39-year-old age group, men were three times more likely than women to develop the disease; after age 40, renal cell carcinoma was diagnosed in men twice as often as in women. Survival probability has increased from 1940 to the present time. A high density of persons per square mile was associated with a higher-than-expected incidence. No trends in incidence according to socioeconomic status were observed.", "PMID": 978762}
{"id": "pubmed23n0032_11859", "title": "Changes with age in the hematopoietic system in a cesarean-derived, barrier-maintained colony of Wistar rats.", "content": "In a colony of cesarean-derived barrier-maintained inbred W rats given saline injections into the right pleural cavities, histologic and cytologic changes due to age were examined in the bone marrow, lymphatic tissue, and lungs; hematologic trends were also evaluated. The thymus showed two changes with age: 1) atrophy, which was evidenced by plasma cells near the blood vessels and among the cords of epithelial cells; and 2) disruption of the normal architecture of the gland. In all available thymuses, changes showed a transition toward malignancy. As the bone marrow showed increasing cellularity with time, polymorphonuclear cells and precursors appeared to increase. In lymph nodes, lymphocytes in the paracortical area were excessive and resembled the lymphocytes of the \"lymphoid bursts\" found in the thymus. In the lungs, small parabronchial and paravascular collections of mononuclear cells, occurring with slightly increasing frequency with age, were consistently found; however, any lung disease associated with these cells was not evident. In the blood, absolute numbers of mononuclear and polymorphonuclear cells were altered.", "contents": "Changes with age in the hematopoietic system in a cesarean-derived, barrier-maintained colony of Wistar rats. In a colony of cesarean-derived barrier-maintained inbred W rats given saline injections into the right pleural cavities, histologic and cytologic changes due to age were examined in the bone marrow, lymphatic tissue, and lungs; hematologic trends were also evaluated. The thymus showed two changes with age: 1) atrophy, which was evidenced by plasma cells near the blood vessels and among the cords of epithelial cells; and 2) disruption of the normal architecture of the gland. In all available thymuses, changes showed a transition toward malignancy. As the bone marrow showed increasing cellularity with time, polymorphonuclear cells and precursors appeared to increase. In lymph nodes, lymphocytes in the paracortical area were excessive and resembled the lymphocytes of the \"lymphoid bursts\" found in the thymus. In the lungs, small parabronchial and paravascular collections of mononuclear cells, occurring with slightly increasing frequency with age, were consistently found; however, any lung disease associated with these cells was not evident. In the blood, absolute numbers of mononuclear and polymorphonuclear cells were altered.", "PMID": 978763}
{"id": "pubmed23n0032_11860", "title": "Hormone-dependent mammary tumors in strain GR/A mice. II. Preneoplastic and neoplastic properties.", "content": "In a series of transplant experiments, we investigated interactions between normal, ductal phase, and tumorous phase mammary tissues in GR/A mice, with particular respect to growth regulation. Transplants from hormone-dependent mammary tumors (HDT) transplanted into mammary fat pads already containing normal mammary ducts usually could not be located subsequently or, at best, displayed minimal growth. Growth regulation was also normal when two HDT transplants, placed in a single gland-free fat pad in nonpregnant hosts, produced ductal outgrowths displaying mutual avoidance behavior in which ducts did not touch and were normally spaced. A normal and HDT transplant in a single gland-free fat pad also showed identical, normal regulatory behavior. In contrast, HDT transplants in pregnant hosts or in hosts receiving exogenous hormone therapy displayed altered growth patterns in which neighboring tumors touched and eventually fused. When surrounded by normal tissues, the HDT continued to proliferate and often appeared to overgrow and engulf normal elements. We concluded that HDT tissues grown in nonpregnant hosts fully responded to those short-range regulatory influences characteristic of normal morphogenesis. When exposed to hormones of pregnancy, however, these interactions changed, and HDT tissues exhibited many characteristics of ductal carcinomas.", "contents": "Hormone-dependent mammary tumors in strain GR/A mice. II. Preneoplastic and neoplastic properties. In a series of transplant experiments, we investigated interactions between normal, ductal phase, and tumorous phase mammary tissues in GR/A mice, with particular respect to growth regulation. Transplants from hormone-dependent mammary tumors (HDT) transplanted into mammary fat pads already containing normal mammary ducts usually could not be located subsequently or, at best, displayed minimal growth. Growth regulation was also normal when two HDT transplants, placed in a single gland-free fat pad in nonpregnant hosts, produced ductal outgrowths displaying mutual avoidance behavior in which ducts did not touch and were normally spaced. A normal and HDT transplant in a single gland-free fat pad also showed identical, normal regulatory behavior. In contrast, HDT transplants in pregnant hosts or in hosts receiving exogenous hormone therapy displayed altered growth patterns in which neighboring tumors touched and eventually fused. When surrounded by normal tissues, the HDT continued to proliferate and often appeared to overgrow and engulf normal elements. We concluded that HDT tissues grown in nonpregnant hosts fully responded to those short-range regulatory influences characteristic of normal morphogenesis. When exposed to hormones of pregnancy, however, these interactions changed, and HDT tissues exhibited many characteristics of ductal carcinomas.", "PMID": 978764}
{"id": "pubmed23n0032_11861", "title": "Hormone-dependent mammary tumors in strain GR/A mice. III. Effectiveness of supplementary hormone treatments in inducing tumorous phase growth.", "content": "Attempts were made to initiate hormone-dependent tumor (HDT) transformation in ductal phase mammary outgrowths. These outgrowths, when carried in virgin GR/A mice, were not tumorous and usually resembled the host's mammary glands. After 2-3 months' initial growth, the following hormone treatments were initiated: 1) An isologous pituitary was implanted into each fat pad having previously received an HDT transplant. Some neoplastic transformation of the pregnancy-dependent tumor outgrowth occurred after 1-2 months; all 10 mammary glands were well stimulated and had many hyperplastic alveolar nodules. 2) In experiments involving exogenous hormone administration, estrogen or progesterone administered alone caused no neoplastic changes in the 2- to 3-month-old ductal phase outgrowths. However, 1 mg progesterone plus 1 mug estrogen daily given sc for 3 weeks to both ovariectomized and intact mice with HDT transplants caused neoplastic changes in the outgrowths and induced small ductal hyperplasias in the host glands. Termination of hormone treatment resulted in a return to the normal ductal structure. The addition of 100 mug prolactin and 100 mug growth hormone to the progesterone and estrogen mixture did not further alter transplants but increased stimulation to the host glands.", "contents": "Hormone-dependent mammary tumors in strain GR/A mice. III. Effectiveness of supplementary hormone treatments in inducing tumorous phase growth. Attempts were made to initiate hormone-dependent tumor (HDT) transformation in ductal phase mammary outgrowths. These outgrowths, when carried in virgin GR/A mice, were not tumorous and usually resembled the host's mammary glands. After 2-3 months' initial growth, the following hormone treatments were initiated: 1) An isologous pituitary was implanted into each fat pad having previously received an HDT transplant. Some neoplastic transformation of the pregnancy-dependent tumor outgrowth occurred after 1-2 months; all 10 mammary glands were well stimulated and had many hyperplastic alveolar nodules. 2) In experiments involving exogenous hormone administration, estrogen or progesterone administered alone caused no neoplastic changes in the 2- to 3-month-old ductal phase outgrowths. However, 1 mg progesterone plus 1 mug estrogen daily given sc for 3 weeks to both ovariectomized and intact mice with HDT transplants caused neoplastic changes in the outgrowths and induced small ductal hyperplasias in the host glands. Termination of hormone treatment resulted in a return to the normal ductal structure. The addition of 100 mug prolactin and 100 mug growth hormone to the progesterone and estrogen mixture did not further alter transplants but increased stimulation to the host glands.", "PMID": 978765}
{"id": "pubmed23n0032_11862", "title": "A proper sequence for the treatment of B16 melanoma: chemotherapy, surgery, and immunotherapy.", "content": "The therapeutic effectiveness of surgery, chemotherapy, and immunotherapy alone and in combination were studied in the B16 melanoma model. The sequence of chemotherapy and immunotherapy as adjuvants to surgery proved important: Chemotherapy was significantly better when given before surgery; immunotherapy was more effective when delivered after surgery. The most effective therapeutic regimen was a combination of all three modalities: a single course of chemotherapy preceding surgery followed by immunotherapy.", "contents": "A proper sequence for the treatment of B16 melanoma: chemotherapy, surgery, and immunotherapy. The therapeutic effectiveness of surgery, chemotherapy, and immunotherapy alone and in combination were studied in the B16 melanoma model. The sequence of chemotherapy and immunotherapy as adjuvants to surgery proved important: Chemotherapy was significantly better when given before surgery; immunotherapy was more effective when delivered after surgery. The most effective therapeutic regimen was a combination of all three modalities: a single course of chemotherapy preceding surgery followed by immunotherapy.", "PMID": 978766}
{"id": "pubmed23n0032_11863", "title": "Inbreeding and canine mammary cancer: a retrospective study.", "content": "Using files of the Animal Neoplasm Registry (ANR) in Alameda and Contra Costa Counties, California, we conducted a retrospective study to compare the degree of inbreeding in the ancestry of purebred dogs with mammary and other cancers, and of those without tumors. Wright's coefficients of inbreeding, calculated for all animals in the three groups, ranged from 0.000 to 0.535. The median inbreeding coefficients of the mammary cancer and comparison groups (consisting of other cancers) were approximately twice that of the nonneoplastic group, but neither difference was statistically significant. Dogs with mammary adenocarcinoma and mixed mammary cancer had similar degrees of inbreeding.", "contents": "Inbreeding and canine mammary cancer: a retrospective study. Using files of the Animal Neoplasm Registry (ANR) in Alameda and Contra Costa Counties, California, we conducted a retrospective study to compare the degree of inbreeding in the ancestry of purebred dogs with mammary and other cancers, and of those without tumors. Wright's coefficients of inbreeding, calculated for all animals in the three groups, ranged from 0.000 to 0.535. The median inbreeding coefficients of the mammary cancer and comparison groups (consisting of other cancers) were approximately twice that of the nonneoplastic group, but neither difference was statistically significant. Dogs with mammary adenocarcinoma and mixed mammary cancer had similar degrees of inbreeding.", "PMID": 978767}
{"id": "pubmed23n0032_11864", "title": "High incidence of spontaneous cervical and vaginal tumors in an inbred strain of Brown Norway rats (BN/Bi).", "content": "The incidence of spontaneously occurring tumors of the cervix and vagina was unusually high in an inbred strain of Brown Norway rats (BN/Bi). Fifty-four tumors were diagnosed in the cervixes or vaginas of 252 rats. Of the tumors, 7 were squamous cell carcinomas, 4 were leiomyomas, and the rest were sarcomas. These tumors were found in nearly 20% of all female rats that died naturally.", "contents": "High incidence of spontaneous cervical and vaginal tumors in an inbred strain of Brown Norway rats (BN/Bi). The incidence of spontaneously occurring tumors of the cervix and vagina was unusually high in an inbred strain of Brown Norway rats (BN/Bi). Fifty-four tumors were diagnosed in the cervixes or vaginas of 252 rats. Of the tumors, 7 were squamous cell carcinomas, 4 were leiomyomas, and the rest were sarcomas. These tumors were found in nearly 20% of all female rats that died naturally.", "PMID": 978768}
{"id": "pubmed23n0032_11865", "title": "Comparison of the potentiation of specific tumor immunity in mice by Corynebacterium parvum or BCG.", "content": "Two independent studies have compared killed C. parvum (CP) vaccine with viable BCG (Pasteur) and BCG (Glaxo) vaccines, respectively, for potentiation of antitumor immunity when injected with irradiated tumor cells into B6D2F1 AND CBA-T6T6 mice. Both studies concurred that, for a given number of irradiated tumor cells, larger amounts (dry weight equivalent) of BCG than CP were required to produce similar degrees of tumor immunity. Evidence also showed that stronger and more protracted immunity was mediated by CP than by BCG.", "contents": "Comparison of the potentiation of specific tumor immunity in mice by Corynebacterium parvum or BCG. Two independent studies have compared killed C. parvum (CP) vaccine with viable BCG (Pasteur) and BCG (Glaxo) vaccines, respectively, for potentiation of antitumor immunity when injected with irradiated tumor cells into B6D2F1 AND CBA-T6T6 mice. Both studies concurred that, for a given number of irradiated tumor cells, larger amounts (dry weight equivalent) of BCG than CP were required to produce similar degrees of tumor immunity. Evidence also showed that stronger and more protracted immunity was mediated by CP than by BCG.", "PMID": 978769}
{"id": "pubmed23n0032_11866", "title": "Spontaneous ovarian tumors in domestic carp (Cyprinus carpio): light and electron microscopy.", "content": "Massive abdominal enlargement was discovered in 8 adult carp (Cyprinus carpio) 4-6 years old, living in breeding ponds of carp fisheries in northern Japan. The abdominal enlargements rapidly progressed and the affected fish died within a few months. At necropsy, single or multiple tumors up to 20 cm in diameter were found in the abdominal cavities. Ovarian tissue identified within the tumor capsule in 4 fish supported the impression, based on gross anatomic features, that the neoplasm arose in the ovaries. Histologically, the tumors were composed mainly of various types of mesenchymal cells. In one sample, striated muscle was identified by electron microscopy; in another, squamous cell nests were intermixed with mesenchymal elements. Although some tumors had areas of cellular pleomorphism suggesting a malignant character, no evidence of invasion or metastasis was found. These neoplasms were classified tentatively as dysontogenetic tumors of the ovary, possibly teratoid in nature.", "contents": "Spontaneous ovarian tumors in domestic carp (Cyprinus carpio): light and electron microscopy. Massive abdominal enlargement was discovered in 8 adult carp (Cyprinus carpio) 4-6 years old, living in breeding ponds of carp fisheries in northern Japan. The abdominal enlargements rapidly progressed and the affected fish died within a few months. At necropsy, single or multiple tumors up to 20 cm in diameter were found in the abdominal cavities. Ovarian tissue identified within the tumor capsule in 4 fish supported the impression, based on gross anatomic features, that the neoplasm arose in the ovaries. Histologically, the tumors were composed mainly of various types of mesenchymal cells. In one sample, striated muscle was identified by electron microscopy; in another, squamous cell nests were intermixed with mesenchymal elements. Although some tumors had areas of cellular pleomorphism suggesting a malignant character, no evidence of invasion or metastasis was found. These neoplasms were classified tentatively as dysontogenetic tumors of the ovary, possibly teratoid in nature.", "PMID": 978770}
{"id": "pubmed23n0032_11867", "title": "A phase I-II trial of multiple-dose polyriboinosic-polyribocytidylic acid in patieonts with leukemia or solid tumors.", "content": "Polyriboinosinic-polyribocytidylic acid (poly I - poly C), an interferon inducer, was administered in multiple doses of 0.3-75 mg/m2 to 26 patients with a variety of solid tumors, 9 with acute leukemia, and 2 with chronic myelogenous leukemia in blast crisis. Forty-four separate drug trials were comprised of various schedules and routes of administration. Toxic reactions included fever (in 66% of the trials), transient elevation of serum glutamic-oxaloacetic transaminase and serum glutamic-pyruvic transaminase (25%), minimal laboratory evidence of coagulation abnormalities (59%), and hypersensitivity (5%). These toxic manifestations did not relate to dose level or magnitude of interferon induction. Poly I - poly C administered iv induced low serum concentrations of interferon in 24/38 trials (63%), but the correlation between drug dose and peak interferon titer was not linear. Poly I - poly C administered iv or im was not effective as an inducer of interferon in the cerebrospinal fluid. Similarly, poly I - poly C administered im or by inhalation did not produce detectable serum levels of interferon. No patients experienced an objective tumor response to the administration of poly I - poly C, and most (76%) had progression of their disease while receiving the drug.", "contents": "A phase I-II trial of multiple-dose polyriboinosic-polyribocytidylic acid in patieonts with leukemia or solid tumors. Polyriboinosinic-polyribocytidylic acid (poly I - poly C), an interferon inducer, was administered in multiple doses of 0.3-75 mg/m2 to 26 patients with a variety of solid tumors, 9 with acute leukemia, and 2 with chronic myelogenous leukemia in blast crisis. Forty-four separate drug trials were comprised of various schedules and routes of administration. Toxic reactions included fever (in 66% of the trials), transient elevation of serum glutamic-oxaloacetic transaminase and serum glutamic-pyruvic transaminase (25%), minimal laboratory evidence of coagulation abnormalities (59%), and hypersensitivity (5%). These toxic manifestations did not relate to dose level or magnitude of interferon induction. Poly I - poly C administered iv induced low serum concentrations of interferon in 24/38 trials (63%), but the correlation between drug dose and peak interferon titer was not linear. Poly I - poly C administered iv or im was not effective as an inducer of interferon in the cerebrospinal fluid. Similarly, poly I - poly C administered im or by inhalation did not produce detectable serum levels of interferon. No patients experienced an objective tumor response to the administration of poly I - poly C, and most (76%) had progression of their disease while receiving the drug.", "PMID": 978771}
{"id": "pubmed23n0032_11868", "title": "Glycoproteins on the surface of neuroblastoma cells.", "content": "Three neuroblastoma systems were used to define fucose-containing glycopeptides on the cell surface and to relate them to the phenotypic expressions of neuronal functions. These systems were a) clonal lines of mouse neuroblastoma C-1300, b) cell hybrids of mouse neuroblastoma and rat glioma, and c) human neuroblastomas, primary cells from the tumor, and cell lines. The results suggest that similarities exist in the membrane glycopeptides available at the surface of the mouse and human cells. It is proposed that these similarities correspond to the ability of the cells to perform the differentiated functions of neuronal cells or to exist as tumors. Based on analogy with other cell membranes, a schema is given for the structure of the membrane glycopeptides on the neuroblastoma cell.", "contents": "Glycoproteins on the surface of neuroblastoma cells. Three neuroblastoma systems were used to define fucose-containing glycopeptides on the cell surface and to relate them to the phenotypic expressions of neuronal functions. These systems were a) clonal lines of mouse neuroblastoma C-1300, b) cell hybrids of mouse neuroblastoma and rat glioma, and c) human neuroblastomas, primary cells from the tumor, and cell lines. The results suggest that similarities exist in the membrane glycopeptides available at the surface of the mouse and human cells. It is proposed that these similarities correspond to the ability of the cells to perform the differentiated functions of neuronal cells or to exist as tumors. Based on analogy with other cell membranes, a schema is given for the structure of the membrane glycopeptides on the neuroblastoma cell.", "PMID": 978772}
{"id": "pubmed23n0032_11869", "title": "Cell kinetics and chemotherapy in neuroblastoma.", "content": "The proliferative behavior of neuroblastoma cells in bone marrow was evaluated before and after perturbation by chemotherapy. The data presented indicated that the proliferating fraction of the tumor cells before therapy was small. In most of the patients, initial therapy with a non-cell-cycle specific agent increased the mitotic and labeling indices. If initial therapy was followed by a cell cycle-specific agent, variable kinetic responses were observed. Although the number of patients was small and the follow-up was short, there was a correlation between the clinical response and the kinetic changes induced by the chemotherapy. Evaluation of cell kinetic changes during the initial course of chemotherapy might identify early those patients who are unlikely to have a good clinical response. Similar studies in the evaluation of new drugs to aid in identifying agents that favorably change cell kinetics and thus therapy for children with neuroblastoma was urged.", "contents": "Cell kinetics and chemotherapy in neuroblastoma. The proliferative behavior of neuroblastoma cells in bone marrow was evaluated before and after perturbation by chemotherapy. The data presented indicated that the proliferating fraction of the tumor cells before therapy was small. In most of the patients, initial therapy with a non-cell-cycle specific agent increased the mitotic and labeling indices. If initial therapy was followed by a cell cycle-specific agent, variable kinetic responses were observed. Although the number of patients was small and the follow-up was short, there was a correlation between the clinical response and the kinetic changes induced by the chemotherapy. Evaluation of cell kinetic changes during the initial course of chemotherapy might identify early those patients who are unlikely to have a good clinical response. Similar studies in the evaluation of new drugs to aid in identifying agents that favorably change cell kinetics and thus therapy for children with neuroblastoma was urged.", "PMID": 978773}
{"id": "pubmed23n0032_11870", "title": "Lymphocyte transformation in children with neuroblastoma.", "content": "Using a simple fingerstick-whole blood technique, we demonstrated that patients with disseminated neuroblastoma have diminished lymphocyte responses to phytohemagglutinin (PHA) and that their sensitivity to antilymphocyte serum inhibition of PHA responses was increased over that of tumor-free controls. These altered immunologic reactivities led us to question the chronologic sequence between the occurrence of the tumor and the lymphocyte defect. Tumor-bearing patients had serum substances that exerted an inhibitory effect on the transformation responses of lymphocytes from unrelated normal individuals; these substances may be present in sera for long periods after the tumor is eliminated.", "contents": "Lymphocyte transformation in children with neuroblastoma. Using a simple fingerstick-whole blood technique, we demonstrated that patients with disseminated neuroblastoma have diminished lymphocyte responses to phytohemagglutinin (PHA) and that their sensitivity to antilymphocyte serum inhibition of PHA responses was increased over that of tumor-free controls. These altered immunologic reactivities led us to question the chronologic sequence between the occurrence of the tumor and the lymphocyte defect. Tumor-bearing patients had serum substances that exerted an inhibitory effect on the transformation responses of lymphocytes from unrelated normal individuals; these substances may be present in sera for long periods after the tumor is eliminated.", "PMID": 978775}
{"id": "pubmed23n0032_11871", "title": "Immunotherapy and chemotherapy in children with neuroblastoma.", "content": "Recent advances with immunotherapy in animal tumors suggested that trials with a combination of chemotherapy and immunotherapy in human malignant tumors might be worthwhile. A pilot program with Vibrio cholera neuraminidase-treated tumor cells plus BCG was tested in 3 patients who had had chemotherapy for disseminated neuroblastoma. Two of these children were in \"complete remission\" after radiation therapy and chemotherapy before the administration of immunotherapy. Relapse occurred in 5-6 months in all 3 patients. These disappointing results are discussed in relation to problems of current chemotherapy in disseminated neuroblastoma including results obtained at second-look operations in patients obtaining \"complete remission.\"", "contents": "Immunotherapy and chemotherapy in children with neuroblastoma. Recent advances with immunotherapy in animal tumors suggested that trials with a combination of chemotherapy and immunotherapy in human malignant tumors might be worthwhile. A pilot program with Vibrio cholera neuraminidase-treated tumor cells plus BCG was tested in 3 patients who had had chemotherapy for disseminated neuroblastoma. Two of these children were in \"complete remission\" after radiation therapy and chemotherapy before the administration of immunotherapy. Relapse occurred in 5-6 months in all 3 patients. These disappointing results are discussed in relation to problems of current chemotherapy in disseminated neuroblastoma including results obtained at second-look operations in patients obtaining \"complete remission.\"", "PMID": 978774}
{"id": "pubmed23n0032_11872", "title": "Reactions to dinitrochlorobenzene in patients with neuroblastoma and survival.", "content": "To determine the relationship of cell-mediated immunity (CMI) to survival, I studied 67 children with the histologically confirmed diagnosis of neuroblastoma. CMI was measured by testing the ability of patients to develop sensitization to dinitrochlorobenzene (DNCB) and to respond to challenge. Reactions at the challenge sites were considered positive only when induration and swelling were present. Ninety percent of the patients with localized neuroblastoma and 27% of those with disseminated neuroblastoma were DNCB reactive. This suggested that patients with DNCB-positive reactions have a better chance of survival than those who are DNCB negative. Some patients with disseminated disease were evaluated in longitudinal studies, and alterations in reactivity did occur with changes in disease status and/or chemotherapy. The suggestion was made that this test systme is potentially useful in estimating survival in children with neuroblastoma.", "contents": "Reactions to dinitrochlorobenzene in patients with neuroblastoma and survival. To determine the relationship of cell-mediated immunity (CMI) to survival, I studied 67 children with the histologically confirmed diagnosis of neuroblastoma. CMI was measured by testing the ability of patients to develop sensitization to dinitrochlorobenzene (DNCB) and to respond to challenge. Reactions at the challenge sites were considered positive only when induration and swelling were present. Ninety percent of the patients with localized neuroblastoma and 27% of those with disseminated neuroblastoma were DNCB reactive. This suggested that patients with DNCB-positive reactions have a better chance of survival than those who are DNCB negative. Some patients with disseminated disease were evaluated in longitudinal studies, and alterations in reactivity did occur with changes in disease status and/or chemotherapy. The suggestion was made that this test systme is potentially useful in estimating survival in children with neuroblastoma.", "PMID": 978776}
{"id": "pubmed23n0032_11873", "title": "Carcinoembryonic antigen in children with neuroblastoma.", "content": "Plasma carcinoembryonic antigen (CEA) was assayed with a radioimmune procedure in 27 healthy control children. The upper limit of plasma CEA (mean +2 SD) was derived from healthy controls and was 3.35 ng/ml. This value was compared with those obtained from 15 children with active neuroblastoma, 7 with active embryonal rhabdomyosarcoma, 16 with treated neuroblastoma and without evidence of disease, 14 disease-free patients with embryonal rhabdomyosarcoma, and 17 patients still on therapy. The neuroblastoma and embryonal rhabdomyosarcoma patients with active disease had higher CEA values than did the successfully treated neuroblastoma and embryonal rhabdomyosarcoma patients. CEA plasma values greater than 3.35 ng/ml were found in 35% and 24% of patiens with neuroblastoma and embryonal rhabdomyosarcoma, respectively.", "contents": "Carcinoembryonic antigen in children with neuroblastoma. Plasma carcinoembryonic antigen (CEA) was assayed with a radioimmune procedure in 27 healthy control children. The upper limit of plasma CEA (mean +2 SD) was derived from healthy controls and was 3.35 ng/ml. This value was compared with those obtained from 15 children with active neuroblastoma, 7 with active embryonal rhabdomyosarcoma, 16 with treated neuroblastoma and without evidence of disease, 14 disease-free patients with embryonal rhabdomyosarcoma, and 17 patients still on therapy. The neuroblastoma and embryonal rhabdomyosarcoma patients with active disease had higher CEA values than did the successfully treated neuroblastoma and embryonal rhabdomyosarcoma patients. CEA plasma values greater than 3.35 ng/ml were found in 35% and 24% of patiens with neuroblastoma and embryonal rhabdomyosarcoma, respectively.", "PMID": 978777}
{"id": "pubmed23n0032_11874", "title": "Recrudescence of neuroblastoma after apparent cure.", "content": "Many years after apparent cure recrudescence of neuroblastoma was reported in 2 patients. In 1 patient, two recurrences occurred 5 and 10 years after apparent disappearance of tumor. Factors contributing to the extraordinary clinical course are unknown. Speculation rests on tumor characteristics, environmental influences, immunity, or the development of a second primary tumor.", "contents": "Recrudescence of neuroblastoma after apparent cure. Many years after apparent cure recrudescence of neuroblastoma was reported in 2 patients. In 1 patient, two recurrences occurred 5 and 10 years after apparent disappearance of tumor. Factors contributing to the extraordinary clinical course are unknown. Speculation rests on tumor characteristics, environmental influences, immunity, or the development of a second primary tumor.", "PMID": 978778}
{"id": "pubmed23n0032_11875", "title": "Homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference focus-forming units.", "content": "Lymphocytic choriomeninigitis (LCM) virus defective interfering (DI) particles form foci of protected cells in a monolayer under an agarose-containing overlay medium. Foci originate from one cell dually infected with at least 1 interference focus-forming unit and infectious virus. As a result, an interfering factor is produced and released which interacts with neighboring cells, thereby protecting them against cytopathic lysis by challenge virus. The property of individual LCM virus DI particles to induce countable foci has been made the basis of quantitative assay that is comparable in every respect to the plaque assay of infectious virus and is much more sensitive and probably more accurate than other procedures used to measure LCM virus DI particles. LCM virus was passaged, undiluted, 10 times in cell cultures. When yields were analyzed as to concentrations of PFU and interference focus-forming units, both entities were found to fluctuate with the pattern expected from theoretical considerations.", "contents": "Homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference focus-forming units. Lymphocytic choriomeninigitis (LCM) virus defective interfering (DI) particles form foci of protected cells in a monolayer under an agarose-containing overlay medium. Foci originate from one cell dually infected with at least 1 interference focus-forming unit and infectious virus. As a result, an interfering factor is produced and released which interacts with neighboring cells, thereby protecting them against cytopathic lysis by challenge virus. The property of individual LCM virus DI particles to induce countable foci has been made the basis of quantitative assay that is comparable in every respect to the plaque assay of infectious virus and is much more sensitive and probably more accurate than other procedures used to measure LCM virus DI particles. LCM virus was passaged, undiluted, 10 times in cell cultures. When yields were analyzed as to concentrations of PFU and interference focus-forming units, both entities were found to fluctuate with the pattern expected from theoretical considerations.", "PMID": 978787}
{"id": "pubmed23n0032_11876", "title": "Interaction of elongation factor 1 with aminoacylated brome mosaic virus and tRNA's.", "content": "Tyrosylated Brome mosaic virus RNA was found to interact with a binary complex of wheat germ, elongation factor 1 and [3H]GTP. Increasing amounts of the aminoacylated viral RNA proportionately reduced radioactivity bound to a nitrocellulose filter, as has previously been noted by others for the charged forms of tobacco mosaic virus, turnip yellow mosaic virus, and tRNA's. However, Sephadex chromatography of the products showed that instead of forming the ternary complex elongation factor-GTP-aminoacyl RNA, the viral RNA caused release of GTP from its complex with elongation factor. Acetylated tyrosyl Brome mosaic virus RNA did not react with the binary complex,and only a slight degree, if any, of stabilization of tyrosine bound to viral RNA was observed after interaction with elongation factor 1. Although such interactions are similar to the reaction of elongation factor with aminoacyl-tRNA , the release of GTP is different and accentuates the possible role for aminoacylation in transcription rather than in translation events.", "contents": "Interaction of elongation factor 1 with aminoacylated brome mosaic virus and tRNA's. Tyrosylated Brome mosaic virus RNA was found to interact with a binary complex of wheat germ, elongation factor 1 and [3H]GTP. Increasing amounts of the aminoacylated viral RNA proportionately reduced radioactivity bound to a nitrocellulose filter, as has previously been noted by others for the charged forms of tobacco mosaic virus, turnip yellow mosaic virus, and tRNA's. However, Sephadex chromatography of the products showed that instead of forming the ternary complex elongation factor-GTP-aminoacyl RNA, the viral RNA caused release of GTP from its complex with elongation factor. Acetylated tyrosyl Brome mosaic virus RNA did not react with the binary complex,and only a slight degree, if any, of stabilization of tyrosine bound to viral RNA was observed after interaction with elongation factor 1. Although such interactions are similar to the reaction of elongation factor with aminoacyl-tRNA , the release of GTP is different and accentuates the possible role for aminoacylation in transcription rather than in translation events.", "PMID": 978788}
{"id": "pubmed23n0032_11877", "title": "Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus.", "content": "Disruption of purified lymphocytic choriomeningitis (LCM) virus with Nonidet P-40 in 0.5 M KCl followed by sucrose gradient centrifugation in 0.3 M KCl led to the isolation of two viral nucleoproteins (RNPs) as well as 40S and 60S ribosomal subunits. The largest viral RNP sedimented heterogenously at 123S to 148S and was associated with 23S and 31S viral RNA. The other viral RNP sedimented at 83S and was associated with 23S viral RNA. The buoyant density in CsCl was determined to be 1.32 g/cm3 for the viral RNP. Densities of 1.52 and 1.60 g/cm3 were determined for the 40S and 60S subunits, similar to those of the BHK-21 cells subunits dissociated by 0.5 M KCl. The viral RNPs were partly sensitive to RNase.", "contents": "Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus. Disruption of purified lymphocytic choriomeningitis (LCM) virus with Nonidet P-40 in 0.5 M KCl followed by sucrose gradient centrifugation in 0.3 M KCl led to the isolation of two viral nucleoproteins (RNPs) as well as 40S and 60S ribosomal subunits. The largest viral RNP sedimented heterogenously at 123S to 148S and was associated with 23S and 31S viral RNA. The other viral RNP sedimented at 83S and was associated with 23S viral RNA. The buoyant density in CsCl was determined to be 1.32 g/cm3 for the viral RNP. Densities of 1.52 and 1.60 g/cm3 were determined for the 40S and 60S subunits, similar to those of the BHK-21 cells subunits dissociated by 0.5 M KCl. The viral RNPs were partly sensitive to RNase.", "PMID": 978789}
{"id": "pubmed23n0032_11878", "title": "Genetic studies of coliphage P1. III. Extended genetic map.", "content": "An extensive genetic map of coliphage P1 has been constructed for 113 amber mutants, using primarily a modification of the conventional complementation spot test. These spot tests failed to classify the mutants into cistrons, but when they were quantitated they permitted assignment of the mutants into 10 linkage clusters. Furthermore, a linear order could be deduced for most of the mutants within each cluster. This strongly suggested that recombination was the predominant event generating plaques and that, for the practical purpose of rapid genetic mapping, such spot tests could be considered as a series of two-factor crosses. Six of the 10 linkage clusters correlated with the P1 genetic map established by Scott (1968). The locations of the remaining four clusters were determined by three-factor crosses and by prophage deletion mapping. The nonrandom occurrence of termini for 14 deletion prophages, which we established previously (Walker and Walker, 1975), and the coincidence of these termini with five out of ten regions demarcating the linkage clusters are discussed. Complementation tests in liquid frequently gave ambiguous results. Therefore, cistron designations were not assigned.", "contents": "Genetic studies of coliphage P1. III. Extended genetic map. An extensive genetic map of coliphage P1 has been constructed for 113 amber mutants, using primarily a modification of the conventional complementation spot test. These spot tests failed to classify the mutants into cistrons, but when they were quantitated they permitted assignment of the mutants into 10 linkage clusters. Furthermore, a linear order could be deduced for most of the mutants within each cluster. This strongly suggested that recombination was the predominant event generating plaques and that, for the practical purpose of rapid genetic mapping, such spot tests could be considered as a series of two-factor crosses. Six of the 10 linkage clusters correlated with the P1 genetic map established by Scott (1968). The locations of the remaining four clusters were determined by three-factor crosses and by prophage deletion mapping. The nonrandom occurrence of termini for 14 deletion prophages, which we established previously (Walker and Walker, 1975), and the coincidence of these termini with five out of ten regions demarcating the linkage clusters are discussed. Complementation tests in liquid frequently gave ambiguous results. Therefore, cistron designations were not assigned.", "PMID": 978790}
{"id": "pubmed23n0032_11879", "title": "Selection and identification of influenza virus recombinants of defined genetic composition.", "content": "The RNAs of influenza virus recombinants were analyzed on polyacrylamide gels under conditions in which the derivation of specific RNA segments (including those coding for hemagglutinin and neuraminidase) could be determined. Analysis of the RNAs of recombinant viruses with identical hemagglutinin and neuramindase revealed that the derivation of the remaining genes could be influenced by UV irradiation of one of the parent viruses. In five of seven such recombinants all of the remaining identifiable genes were derived from the nonirradiated parent, whereas in two others only the three largest RNA segments were derived from the nonirradiated parent. Analysis of the RNA pattern of a recombinant isolated from mixed infection in which neither parent was irradiated demonstrated a random mixture of RNA segments derived from the two parent viruses.", "contents": "Selection and identification of influenza virus recombinants of defined genetic composition. The RNAs of influenza virus recombinants were analyzed on polyacrylamide gels under conditions in which the derivation of specific RNA segments (including those coding for hemagglutinin and neuraminidase) could be determined. Analysis of the RNAs of recombinant viruses with identical hemagglutinin and neuramindase revealed that the derivation of the remaining genes could be influenced by UV irradiation of one of the parent viruses. In five of seven such recombinants all of the remaining identifiable genes were derived from the nonirradiated parent, whereas in two others only the three largest RNA segments were derived from the nonirradiated parent. Analysis of the RNA pattern of a recombinant isolated from mixed infection in which neither parent was irradiated demonstrated a random mixture of RNA segments derived from the two parent viruses.", "PMID": 978791}
{"id": "pubmed23n0032_11880", "title": "mRNA from the transforming segment of the adenovirus 2 genome in productively infected and transformed cells.", "content": "We have identified two mRNA species transcribed from the adenovirus 2 genome section (HindIII-G fragment) believed to harbor genes for initiation and maintenance of cell transformation. The HindIII-G fragment occupies the left 7.5% of the genome and is transcribed from left to right [poly(U:G) r strand]. Poly(A)-terminated labeled mRNA was isolated from polyribosomes of adenovirus 2 early infected KB cells and from the transformed cell line 8617, hybridization purified using the HindIII-G fragment, and electrophoresed on formamide-polyacrylamide gels. Viral mRNA's of 24S (1.2 X 10(6) daltons) and 14S (4.5 X 10(5) daltons) were isolated from early infected cells and of 22S (1.0 X 10(6) daltons) and 14S from 8617 cells. Hybridization competition indicated that HindIII-G-specific mRNA was present in the polysomes at one-sixth the concentration late after infection as compared with early, indicating that the proteins coded by the transforming segment may be synthesized at reduced amounts during late stages. Only 1/10 the amount of RNA labeled late annealed to the G fragment as compared with that labeled early (per weight of RNA). Thus, synthesis of transforming gene mRNA is probably \"turned off\" late after infection. Both 24S (22S) and 14S mRNA's from infected and 8617 cells were complementary to the Hpa I-E fragment (left 4.1% of genome). The Hpa I-E fragment is too small to encode 24S and 14S species, which implies that the 5'-terminal regions of both species are coded by the same DNA sequences.", "contents": "mRNA from the transforming segment of the adenovirus 2 genome in productively infected and transformed cells. We have identified two mRNA species transcribed from the adenovirus 2 genome section (HindIII-G fragment) believed to harbor genes for initiation and maintenance of cell transformation. The HindIII-G fragment occupies the left 7.5% of the genome and is transcribed from left to right [poly(U:G) r strand]. Poly(A)-terminated labeled mRNA was isolated from polyribosomes of adenovirus 2 early infected KB cells and from the transformed cell line 8617, hybridization purified using the HindIII-G fragment, and electrophoresed on formamide-polyacrylamide gels. Viral mRNA's of 24S (1.2 X 10(6) daltons) and 14S (4.5 X 10(5) daltons) were isolated from early infected cells and of 22S (1.0 X 10(6) daltons) and 14S from 8617 cells. Hybridization competition indicated that HindIII-G-specific mRNA was present in the polysomes at one-sixth the concentration late after infection as compared with early, indicating that the proteins coded by the transforming segment may be synthesized at reduced amounts during late stages. Only 1/10 the amount of RNA labeled late annealed to the G fragment as compared with that labeled early (per weight of RNA). Thus, synthesis of transforming gene mRNA is probably \"turned off\" late after infection. Both 24S (22S) and 14S mRNA's from infected and 8617 cells were complementary to the Hpa I-E fragment (left 4.1% of genome). The Hpa I-E fragment is too small to encode 24S and 14S species, which implies that the 5'-terminal regions of both species are coded by the same DNA sequences.", "PMID": 978792}
{"id": "pubmed23n0032_11881", "title": "Size, subunit composition, and secondary structure of the Friend virus genome.", "content": "Electron microscope and gel electrophoresis studies show that the high-molecular-weight (50 to 70S) RNA extract from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD-114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD-114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 +/- 0.6, 7.7 +/- 0.6, and 9.5 +/- 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.", "contents": "Size, subunit composition, and secondary structure of the Friend virus genome. Electron microscope and gel electrophoresis studies show that the high-molecular-weight (50 to 70S) RNA extract from Friend virus (FV) is a dimer with the same basic structure previously observed for the RNAs from RD-114 virus, baboon virus, and woolly monkey virus. This observation greatly strengthens the inference that the dimer structure is a general characteristic of the RNAs of all mammalian type C viruses. The FV dimer is slightly less stable than the RNA dimer of woolly monkey virus, which is, in turn, much less stable than those of RD-114 and baboon virus. There are three FV monomer components, small (S), medium (M), and large (L), with molecular lengths of 6.7 +/- 0.6, 7.7 +/- 0.6, and 9.5 +/- 0.6 kilobases, respectively. There are approximately equal amounts of the S and M components and much less of the L component. Most of the dimers are homodimers (SS, MM, and LL). The frequency of heterodimers (SM, SL, ML) is much less than expected for a random assortment model.", "PMID": 978793}
{"id": "pubmed23n0032_11882", "title": "Multiplication of parvovirus LuIII in a synchronized culture system. IV. Association of viral structural polypeptides with the host cell chromatin.", "content": "Newly synthesized structural polypeptides of parvovirus LuIII, VP1 (62,000 daltons) and VP2 (74,000 daltons), were detected in nuclei of synchronized, infected HeLa cells at 11 to 12 h postinfection, i.e., after cells had passed through the S phase of the cell cycle. At this time, most of intranuclear viral polypeptides were associated with the chromatin acidic proteins. However, 13 to 14 h postinfection, about one-third of intranuclear VP1 and VP2 also could be extracted in the fraction containing nuclear sap proteins. According to pulse-chase experiments, VP1 and VP2 accumulated in the chromatin with a time lag of 20 to 30 min. About 90% of these chromatin-associated viral polypeptides represented empty viral capsids. In addition, chromatin prepared at 14 h postinfection contained 90 to 95% of the total intranuclear viral 16S replicative-form DNA. Since viral replicative-form DNA and empty viral capsids seem to be associated specifically with cellular chromatin, we assume that this subnuclear structure is the site of the synthesis of progeny viral DNA and the formation of complete virions.", "contents": "Multiplication of parvovirus LuIII in a synchronized culture system. IV. Association of viral structural polypeptides with the host cell chromatin. Newly synthesized structural polypeptides of parvovirus LuIII, VP1 (62,000 daltons) and VP2 (74,000 daltons), were detected in nuclei of synchronized, infected HeLa cells at 11 to 12 h postinfection, i.e., after cells had passed through the S phase of the cell cycle. At this time, most of intranuclear viral polypeptides were associated with the chromatin acidic proteins. However, 13 to 14 h postinfection, about one-third of intranuclear VP1 and VP2 also could be extracted in the fraction containing nuclear sap proteins. According to pulse-chase experiments, VP1 and VP2 accumulated in the chromatin with a time lag of 20 to 30 min. About 90% of these chromatin-associated viral polypeptides represented empty viral capsids. In addition, chromatin prepared at 14 h postinfection contained 90 to 95% of the total intranuclear viral 16S replicative-form DNA. Since viral replicative-form DNA and empty viral capsids seem to be associated specifically with cellular chromatin, we assume that this subnuclear structure is the site of the synthesis of progeny viral DNA and the formation of complete virions.", "PMID": 978794}
{"id": "pubmed23n0032_11883", "title": "Read-through proteins of group 4 RNA bacteriophages TW19 and TW28.", "content": "Group 4 phages TW19 and TW28 of Escherichia coli possess a \"read-through\" (IIb) protein, although group 2 phage GA does not. This may have implications concerning the evolution and classification of RNA phages.", "contents": "Read-through proteins of group 4 RNA bacteriophages TW19 and TW28. Group 4 phages TW19 and TW28 of Escherichia coli possess a \"read-through\" (IIb) protein, although group 2 phage GA does not. This may have implications concerning the evolution and classification of RNA phages.", "PMID": 978795}
{"id": "pubmed23n0032_11884", "title": "Recombination and complementation between temperature-sensitive mutants of a Bunyavirus, snowshoe hare virus.", "content": "Complementation and recombination have been observed with temperature-sensitive mutants of Bunyavirus snowshoe hare virus.", "contents": "Recombination and complementation between temperature-sensitive mutants of a Bunyavirus, snowshoe hare virus. Complementation and recombination have been observed with temperature-sensitive mutants of Bunyavirus snowshoe hare virus.", "PMID": 978796}
{"id": "pubmed23n0032_11885", "title": "Mapping of two isoleucine tRNA isoacceptor genes in bacteriophage T5 DNA.", "content": "T5 bacteriophage codes for the synthesis of more than 14 different tRNA species, which map in four separate clusters in the C segment of the T5 chromosome. In this study, two tRNAile isoacceptor species have been identified by reverse-phase chromatography and shown to be transcribed from two different tRNA loci along the T5 chromosome. The map positions of the tRNA isoacceptors were aided by the use of several T5 deletion mutants in which the position and size of the deleted DNA segments had been previously determined by heteroduplex mapping. Hybridization analysis suggests the presence of some sequence homology between the two tRNAile species.", "contents": "Mapping of two isoleucine tRNA isoacceptor genes in bacteriophage T5 DNA. T5 bacteriophage codes for the synthesis of more than 14 different tRNA species, which map in four separate clusters in the C segment of the T5 chromosome. In this study, two tRNAile isoacceptor species have been identified by reverse-phase chromatography and shown to be transcribed from two different tRNA loci along the T5 chromosome. The map positions of the tRNA isoacceptors were aided by the use of several T5 deletion mutants in which the position and size of the deleted DNA segments had been previously determined by heteroduplex mapping. Hybridization analysis suggests the presence of some sequence homology between the two tRNAile species.", "PMID": 978797}
{"id": "pubmed23n0032_11886", "title": "Multiple methylated cap sequences in adenovirus type 2 early mRNA.", "content": "The methylated constituents of early adenovirus 2 mRNA were studied. RNA was isolated from polyribosomes of cells double labeled with [methyl-3H]methionine and 32PO4 from 2 to 7 g postinfection in the presence of cycloheximide. Cycloheximide ensures that methylation and processing are performed by preexisting host cell enzymes. RNA was fractionated into polyadenylic [poly(A)]+ and poly(A)- molecules using poly(U)-Sepharose, and undergraded virus-specific RNA was isolated by hybridization to viral DNA in 50% formamide at 37 degrees C. Viral mRNA was digested with RNase T2 and chromatographed on DEAE-Sephadex in 7 M urea. Two 3H-labeled RNase T2-resistant oligonucleotide fractions with charges between -5 and -6 were obtained, consistent with two classes of 5' terminal methyl \"cap\" structures, m7G(5')ppp(5')NmpNp (cap 1) and m7G(5')ppp(5')NmNmpNp (cap 2) (Nm is a ribose 2'-O-methylation). The putative cap 1 contains all the methylated constituents of cap 1 plus Cm. The molar ratios of m7G to 2'-O-methylnucleosides is about 1.0 for cap 1 and 0.5 for cap 2, consistent with the proposed cap structures. Most significant, compositional analysis indicates four different cap 1 structures and at least three different cap 2 structures. Thus there is a minimum of seven early viral mRNA species with different cap structures, unless each type of mRNA can have more than one 5' terminus. In addition to methylated caps, early mRNA contains internal base methylations, exclusively as m6A, as shown by analyses of the mononucleotide (-2 charge) fraction. m6A was present in the ratio of 1 mol of m6Ap per 450 nucleotides. Thus viral mRNA molecules contain two to three internal m6A residues per methyl cap, since there is on the average 1 cap per 1,250 nucleotides.", "contents": "Multiple methylated cap sequences in adenovirus type 2 early mRNA. The methylated constituents of early adenovirus 2 mRNA were studied. RNA was isolated from polyribosomes of cells double labeled with [methyl-3H]methionine and 32PO4 from 2 to 7 g postinfection in the presence of cycloheximide. Cycloheximide ensures that methylation and processing are performed by preexisting host cell enzymes. RNA was fractionated into polyadenylic [poly(A)]+ and poly(A)- molecules using poly(U)-Sepharose, and undergraded virus-specific RNA was isolated by hybridization to viral DNA in 50% formamide at 37 degrees C. Viral mRNA was digested with RNase T2 and chromatographed on DEAE-Sephadex in 7 M urea. Two 3H-labeled RNase T2-resistant oligonucleotide fractions with charges between -5 and -6 were obtained, consistent with two classes of 5' terminal methyl \"cap\" structures, m7G(5')ppp(5')NmpNp (cap 1) and m7G(5')ppp(5')NmNmpNp (cap 2) (Nm is a ribose 2'-O-methylation). The putative cap 1 contains all the methylated constituents of cap 1 plus Cm. The molar ratios of m7G to 2'-O-methylnucleosides is about 1.0 for cap 1 and 0.5 for cap 2, consistent with the proposed cap structures. Most significant, compositional analysis indicates four different cap 1 structures and at least three different cap 2 structures. Thus there is a minimum of seven early viral mRNA species with different cap structures, unless each type of mRNA can have more than one 5' terminus. In addition to methylated caps, early mRNA contains internal base methylations, exclusively as m6A, as shown by analyses of the mononucleotide (-2 charge) fraction. m6A was present in the ratio of 1 mol of m6Ap per 450 nucleotides. Thus viral mRNA molecules contain two to three internal m6A residues per methyl cap, since there is on the average 1 cap per 1,250 nucleotides.", "PMID": 978798}
{"id": "pubmed23n0032_11887", "title": "Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA.", "content": "The 42S RNA from Semliki Forest virus contains a polyadenylate [poly(A)] sequence that is 80 to 90 residues long and is the 3'-terminus of the virion RNA. A poly(A) sequence of the same length was found in the plus strand of the replicative forms (RFs) and replicative intermediates (RIs) isolated 2 h after infection. In addition, both RFs and RIs contained a polyuridylate [poly(U)] sequence. No poly(U) was found in virion RNA, and thus the poly(U) sequence is in minus-strand RNA. The poly(U) from RFs was on the average 60 residues long, whereas that isolated from the RIs was 80 residues long. Poly(U) sequences isolated from RFs and RIs by digestion with RNase T1 contained 5'-phosphorylated pUp and ppUp residues, indicating that the poly(U) sequence was the 5'-terminus of the minus-strand RNA. The poly(U) sequence in RFs or RIs was free to bind to poly(A)-Sepharose only after denaturation of the RNAs, indicating that the poly(U) was hydrogen bonded to the poly(A) at the 3'-terminus of the plus-strand RNA in these molecules. When treated with 0.02 mug of RNase A per ml, both RFs and RIs yielded the same distribution of the three cores, RFI, RFII, and RFIII. The minus-strand RNA of both RFI and RFIII contained a poly(U) sequence. That from RFII did not. It is known that RFI is the double-stranded form of the 42S plus-strand RNA and that RFIII is the experimetnally derived double-stranded form of 26S mRNA. The poly(A) sequences in each are most likely transcribed directly from the poly(U) at the 5'-end of the 42S minus-strand RNA. The 26S mRNA thus represents the nucleotide sequence in that one-third of the 42S plus-strand RNA that includes its 3'-terminus.", "contents": "Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA. The 42S RNA from Semliki Forest virus contains a polyadenylate [poly(A)] sequence that is 80 to 90 residues long and is the 3'-terminus of the virion RNA. A poly(A) sequence of the same length was found in the plus strand of the replicative forms (RFs) and replicative intermediates (RIs) isolated 2 h after infection. In addition, both RFs and RIs contained a polyuridylate [poly(U)] sequence. No poly(U) was found in virion RNA, and thus the poly(U) sequence is in minus-strand RNA. The poly(U) from RFs was on the average 60 residues long, whereas that isolated from the RIs was 80 residues long. Poly(U) sequences isolated from RFs and RIs by digestion with RNase T1 contained 5'-phosphorylated pUp and ppUp residues, indicating that the poly(U) sequence was the 5'-terminus of the minus-strand RNA. The poly(U) sequence in RFs or RIs was free to bind to poly(A)-Sepharose only after denaturation of the RNAs, indicating that the poly(U) was hydrogen bonded to the poly(A) at the 3'-terminus of the plus-strand RNA in these molecules. When treated with 0.02 mug of RNase A per ml, both RFs and RIs yielded the same distribution of the three cores, RFI, RFII, and RFIII. The minus-strand RNA of both RFI and RFIII contained a poly(U) sequence. That from RFII did not. It is known that RFI is the double-stranded form of the 42S plus-strand RNA and that RFIII is the experimetnally derived double-stranded form of 26S mRNA. The poly(A) sequences in each are most likely transcribed directly from the poly(U) at the 5'-end of the 42S minus-strand RNA. The 26S mRNA thus represents the nucleotide sequence in that one-third of the 42S plus-strand RNA that includes its 3'-terminus.", "PMID": 978799}
{"id": "pubmed23n0032_11888", "title": "Genome expression and mRNA maturation at late stages of productive adenovirus type 2 infection.", "content": "RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand from which they originated. Early whole cell RNA extracted 5 h postinfection annealed rapidly to 10 to 15% of l and r strands and then slowly to final values of 60 and 40% of l and r strands. By 9 h postinfection the expression of late genes was apparent and whole cell RNA annealed to 20 and 75% of l and r strands. Whole cell RNA extracted between 12 and 36 h postinfection annealed to 7 to 15% and 75 to 90% of l and r strands. Late nuclear RNA hybridized to 10 and 90% of l and r strands, and late polyribosomal RNA hybridized to 20 and 75% of l and r strands. Based upon kinetic analyses, we estimate that mRNA synthesized exclusively during late stages arises from about 6 to 8% and 45 to 49% of l and r strands. This assumes that the early class I mRNA (in low concentration late) originates from 8 to 10% and 6 to 10% of l and r strands and that early class II mRNA (in high concentration late) is derived from 2% and 8 to 13% of l and r strands. Mixing experiments indicated that early mRNA is a subset of RNA extracted from polyribosomes late after infection and that late nuclear RNA contains sequences complementary to early l strand class I nRNA. RNA-RNA hybrids were isolated from late mRNA containing sequences from 60% of l and r strands, but it is not known when these were synthesized, and therefore whether complementary RNA transcripts are synthesized late after infection, as they are known to be synthesized early. These results demonstrate that portions of the genome are transcribed into RNA sequences that remain confined to the nucleus and are not exported to polyribosomes as mRNA.", "contents": "Genome expression and mRNA maturation at late stages of productive adenovirus type 2 infection. RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand from which they originated. Early whole cell RNA extracted 5 h postinfection annealed rapidly to 10 to 15% of l and r strands and then slowly to final values of 60 and 40% of l and r strands. By 9 h postinfection the expression of late genes was apparent and whole cell RNA annealed to 20 and 75% of l and r strands. Whole cell RNA extracted between 12 and 36 h postinfection annealed to 7 to 15% and 75 to 90% of l and r strands. Late nuclear RNA hybridized to 10 and 90% of l and r strands, and late polyribosomal RNA hybridized to 20 and 75% of l and r strands. Based upon kinetic analyses, we estimate that mRNA synthesized exclusively during late stages arises from about 6 to 8% and 45 to 49% of l and r strands. This assumes that the early class I mRNA (in low concentration late) originates from 8 to 10% and 6 to 10% of l and r strands and that early class II mRNA (in high concentration late) is derived from 2% and 8 to 13% of l and r strands. Mixing experiments indicated that early mRNA is a subset of RNA extracted from polyribosomes late after infection and that late nuclear RNA contains sequences complementary to early l strand class I nRNA. RNA-RNA hybrids were isolated from late mRNA containing sequences from 60% of l and r strands, but it is not known when these were synthesized, and therefore whether complementary RNA transcripts are synthesized late after infection, as they are known to be synthesized early. These results demonstrate that portions of the genome are transcribed into RNA sequences that remain confined to the nucleus and are not exported to polyribosomes as mRNA.", "PMID": 978800}
{"id": "pubmed23n0032_11889", "title": "Nature of the single-stranded DNA in replicating adenovirus type 5 DNA.", "content": "Single-stranded regions in replicating adenovirus type 5 DNA were isolated and hybridized in solution to the separated strands of adenovirus 2 or 5 DNA. The results showed that the two strands of adenovirus 5 DNA are exposed to almost the same extent during replication, suggesting that displacement synthesis may start from either end of the viral DNA.", "contents": "Nature of the single-stranded DNA in replicating adenovirus type 5 DNA. Single-stranded regions in replicating adenovirus type 5 DNA were isolated and hybridized in solution to the separated strands of adenovirus 2 or 5 DNA. The results showed that the two strands of adenovirus 5 DNA are exposed to almost the same extent during replication, suggesting that displacement synthesis may start from either end of the viral DNA.", "PMID": 978801}
{"id": "pubmed23n0032_11890", "title": "Hypernephroma in the solitary kidney: experience with 20 cases and review of the literature.", "content": "Twenty patients with renal cell carcinoma in anatomically or functionally solitary kidneys were treated and followed for up to 18 years. Factors pertinent to management and survival of these patients and 66 other well documented similar patients reported in the literature are analyzed. Most of the patients were unusually young and a significant number had had nephrectomy for contralateral renal cell carcinoma. Survival was closely related to the earlier presence of malignant disease in the other kidney, the duration of the interval between detection of the 2 neoplasms and the stage of the lesion in the solitary kidney. Partial nephrectomy has been the most successful treatment. The mean survival in the Mayo Clinic series has been 6 years for patients still alive and 2.4 years for those dead at the time of this analysis. Results in this and other series emphasize the importance of thorough long-term followup after nephrectomy for hypernephroma and of aggressive therapy when the remaining kidney becomes involved. It is foolhardy to abandon hope merely because there is a malignant tumor in a solitary kidney.", "contents": "Hypernephroma in the solitary kidney: experience with 20 cases and review of the literature. Twenty patients with renal cell carcinoma in anatomically or functionally solitary kidneys were treated and followed for up to 18 years. Factors pertinent to management and survival of these patients and 66 other well documented similar patients reported in the literature are analyzed. Most of the patients were unusually young and a significant number had had nephrectomy for contralateral renal cell carcinoma. Survival was closely related to the earlier presence of malignant disease in the other kidney, the duration of the interval between detection of the 2 neoplasms and the stage of the lesion in the solitary kidney. Partial nephrectomy has been the most successful treatment. The mean survival in the Mayo Clinic series has been 6 years for patients still alive and 2.4 years for those dead at the time of this analysis. Results in this and other series emphasize the importance of thorough long-term followup after nephrectomy for hypernephroma and of aggressive therapy when the remaining kidney becomes involved. It is foolhardy to abandon hope merely because there is a malignant tumor in a solitary kidney.", "PMID": 978802}
{"id": "pubmed23n0032_11891", "title": "Vesicoureteral reflux and end stage renal disease.", "content": "Thirty per cent of 50 consecutive patients with end stage renal disease were found to have vesicoureteral reflux. This finding is at variance with the usually published etiologies of end stage renal disease. Forty per cent of the patients with reflux had no history to suggest the presence of this entity. The clinical importance of reflux in the post-transplant period as well as its possible etiologic role in end stage renal disease is discussed.", "contents": "Vesicoureteral reflux and end stage renal disease. Thirty per cent of 50 consecutive patients with end stage renal disease were found to have vesicoureteral reflux. This finding is at variance with the usually published etiologies of end stage renal disease. Forty per cent of the patients with reflux had no history to suggest the presence of this entity. The clinical importance of reflux in the post-transplant period as well as its possible etiologic role in end stage renal disease is discussed.", "PMID": 978803}
{"id": "pubmed23n0032_11892", "title": "The surgical fate of ureteral calculi: review of Mayo Clinic experience.", "content": "A review of 1,061 patients who underwent ureterolithotomy of transurethral manipulation revealed 2 factors that are important to consider; 1) the roentgenographic size of the calculi measured at the largest diameter and 2) the location of the calculus. These 2 facotrs in addition to the general medical condition of the patient, the duration of severity of symptoms and the status of the upper urinary tract provide suitable guide lines for individual management of ureteral calculi.", "contents": "The surgical fate of ureteral calculi: review of Mayo Clinic experience. A review of 1,061 patients who underwent ureterolithotomy of transurethral manipulation revealed 2 factors that are important to consider; 1) the roentgenographic size of the calculi measured at the largest diameter and 2) the location of the calculus. These 2 facotrs in addition to the general medical condition of the patient, the duration of severity of symptoms and the status of the upper urinary tract provide suitable guide lines for individual management of ureteral calculi.", "PMID": 978804}
{"id": "pubmed23n0032_11893", "title": "Gunshot wounds of the ureter: a 15-year review of 63 consecutive cases.", "content": "We reviewed 63 consecutive cases of gunshot wounds of the ureter seen during a 15-year period. The importance of prompt recognition and aggressive surgical management is emphasized, with particular attention being placed on adequate debridement and spatulated splinted watertight ureteroureterostomy. Our philosophy of management for individual injuries is discussed along with our complications and results. This series is the largest reported to date of ureteral gunshot wounds.", "contents": "Gunshot wounds of the ureter: a 15-year review of 63 consecutive cases. We reviewed 63 consecutive cases of gunshot wounds of the ureter seen during a 15-year period. The importance of prompt recognition and aggressive surgical management is emphasized, with particular attention being placed on adequate debridement and spatulated splinted watertight ureteroureterostomy. Our philosophy of management for individual injuries is discussed along with our complications and results. This series is the largest reported to date of ureteral gunshot wounds.", "PMID": 978805}
{"id": "pubmed23n0032_11894", "title": "Idiopathic rupture of the bladder.", "content": "Non-traumatic spontaneous rupture of the otherwise normal bladder is a rare occurrence. The literature is reviewed and 4 new cases are reported. Experimental data are presented and a theory is reviewed regarding the frequent intraperitoneal location of the rupture. The frequent paucity of symptoms compared to the high mortality rate is stressed.", "contents": "Idiopathic rupture of the bladder. Non-traumatic spontaneous rupture of the otherwise normal bladder is a rare occurrence. The literature is reviewed and 4 new cases are reported. Experimental data are presented and a theory is reviewed regarding the frequent intraperitoneal location of the rupture. The frequent paucity of symptoms compared to the high mortality rate is stressed.", "PMID": 978806}
{"id": "pubmed23n0032_11895", "title": "Urine cytology in the detection of bladder tumor recurrence.", "content": "Of 118 patients with primary bladder tumors seen since 1966, 73 have been followed with urine cytology since 1969. Of the 406 tests there have been 85 positive, 296 negative and 25 ambiguous reports. The incidence of falsely positive results is estimated at 4% but the incidence of falsely negative results cannot be assessed in this study. Currently, 51 patients are living, 2 of whom had been seen in 1966. Of the 51 patients 43 are being followed with urine cytology. Bimonthly urine cytology has been found to be a relaible, convenient, safe, less hazardous and less costly method for the detection of bladder tumor recurrence.", "contents": "Urine cytology in the detection of bladder tumor recurrence. Of 118 patients with primary bladder tumors seen since 1966, 73 have been followed with urine cytology since 1969. Of the 406 tests there have been 85 positive, 296 negative and 25 ambiguous reports. The incidence of falsely positive results is estimated at 4% but the incidence of falsely negative results cannot be assessed in this study. Currently, 51 patients are living, 2 of whom had been seen in 1966. Of the 51 patients 43 are being followed with urine cytology. Bimonthly urine cytology has been found to be a relaible, convenient, safe, less hazardous and less costly method for the detection of bladder tumor recurrence.", "PMID": 978807}
{"id": "pubmed23n0032_11896", "title": "Partial cystectomy in the treatment of primary and secondary carcinoma of the bladder.", "content": "We reviewed 50 patients who had undergone partial cystectomy for primary carcinoma of the bladder. Specific indications and operative technique are discussed. The 5-year survival rate was 67% for patients with stages O and A disease, 53% for stage B and 20% for stages C and D1. Survival was improved for patients with tumors on the posterior bladder wall. Patients in whom free margins of resection were accomplished for unifocal disease had longer survival, with a diminished propensity for developing recurrent bladder cancer. There was no hospital mortality and postoperative complications were minimal. An additional 12 patients underwent partial cystectomy for adjacent carcinomas secondarily invading the bladder with less favorable results.", "contents": "Partial cystectomy in the treatment of primary and secondary carcinoma of the bladder. We reviewed 50 patients who had undergone partial cystectomy for primary carcinoma of the bladder. Specific indications and operative technique are discussed. The 5-year survival rate was 67% for patients with stages O and A disease, 53% for stage B and 20% for stages C and D1. Survival was improved for patients with tumors on the posterior bladder wall. Patients in whom free margins of resection were accomplished for unifocal disease had longer survival, with a diminished propensity for developing recurrent bladder cancer. There was no hospital mortality and postoperative complications were minimal. An additional 12 patients underwent partial cystectomy for adjacent carcinomas secondarily invading the bladder with less favorable results.", "PMID": 978808}
{"id": "pubmed23n0032_11897", "title": "Non-invasive papillary carcinoma of the bladder associated with carcinoma in situ.", "content": "We evaluated 129 patients with low grade, low stage transitional cell carcinoma of the bladder with a minimum followup of 5 years. In addition to the phenomena recognized as indicators of a serious clinical problem (grade greater than I, rapid recurrence, multiple tumors and lamina propria invasion) the identification of carcinoma in situ and/or atypia in normal, incidentally resected mucosa adjacent to the tumor was found to be a positive predictor for subsequent muscle invasion. Ten of 12 patients with carcinoma in situ and 9 of 25 patients with atypia were among 39 who experienced invasion.", "contents": "Non-invasive papillary carcinoma of the bladder associated with carcinoma in situ. We evaluated 129 patients with low grade, low stage transitional cell carcinoma of the bladder with a minimum followup of 5 years. In addition to the phenomena recognized as indicators of a serious clinical problem (grade greater than I, rapid recurrence, multiple tumors and lamina propria invasion) the identification of carcinoma in situ and/or atypia in normal, incidentally resected mucosa adjacent to the tumor was found to be a positive predictor for subsequent muscle invasion. Ten of 12 patients with carcinoma in situ and 9 of 25 patients with atypia were among 39 who experienced invasion.", "PMID": 978809}
{"id": "pubmed23n0032_11898", "title": "Treatment of urethral syndrome with triamcinolone acetonide.", "content": "A new method for the treatment of the urethral syndrome is presented. Therapy involves the submucosal injection of triamcinolone acetonide around Skene's glands to decrease inflammation and scarring. It is a simple office procedure, while all other therapeutic modalities are surgical.", "contents": "Treatment of urethral syndrome with triamcinolone acetonide. A new method for the treatment of the urethral syndrome is presented. Therapy involves the submucosal injection of triamcinolone acetonide around Skene's glands to decrease inflammation and scarring. It is a simple office procedure, while all other therapeutic modalities are surgical.", "PMID": 978810}
{"id": "pubmed23n0032_11899", "title": "Congenital anomalies associated with hypospadias.", "content": "We did a retrospective survey on 200 patients with hypospadias to determine what other congenital anomalies were present. The 56 patients with associated anomalies included 16 who had abnormal excretory urograms and 10 of these 16 patients had defects requiring surgical correction.", "contents": "Congenital anomalies associated with hypospadias. We did a retrospective survey on 200 patients with hypospadias to determine what other congenital anomalies were present. The 56 patients with associated anomalies included 16 who had abnormal excretory urograms and 10 of these 16 patients had defects requiring surgical correction.", "PMID": 978811}
{"id": "pubmed23n0032_11900", "title": "Urological care of patients with acute spinal cord injury using tidal drainage.", "content": "The function of urological care of patients with spinal cord injuries is is 3-fold: 1) prevention of complications until the bladder has recovered, 2) surgical and medical intervention when recovery is not spontaneous and 3) diagnosis and treatment of complications and/or sequelae. Tidal drainage in these patients is easy, convenient, economical, and readily available and adaptable for use at any institution.", "contents": "Urological care of patients with acute spinal cord injury using tidal drainage. The function of urological care of patients with spinal cord injuries is is 3-fold: 1) prevention of complications until the bladder has recovered, 2) surgical and medical intervention when recovery is not spontaneous and 3) diagnosis and treatment of complications and/or sequelae. Tidal drainage in these patients is easy, convenient, economical, and readily available and adaptable for use at any institution.", "PMID": 978812}
{"id": "pubmed23n0032_11901", "title": "Altered bladder function and non-specific epididymitis.", "content": "Disorders of bladder function characterized by physiological urethral obstruction were identified in nearly 50% of patients with non-specific epididymitis and anatomically normaly urinary tracts. This association supports the concept that reflux of urine into the ejaculatory ducts may occur and produce epididymitis. The pathophysiology of retrograde urination is reviewed and the predisposing conditions are discussed.", "contents": "Altered bladder function and non-specific epididymitis. Disorders of bladder function characterized by physiological urethral obstruction were identified in nearly 50% of patients with non-specific epididymitis and anatomically normaly urinary tracts. This association supports the concept that reflux of urine into the ejaculatory ducts may occur and produce epididymitis. The pathophysiology of retrograde urination is reviewed and the predisposing conditions are discussed.", "PMID": 978813}
{"id": "pubmed23n0032_11902", "title": "The Doppler stethoscope and the diagnosis of the acute scrotum.", "content": "We evaluated 30 patients who had acute scrotal swelling with the Doppler stethoscope. Every case of epididymitis and torsion of the appendix testis demonstrated an increased testicular blood flow. The cases of spermatic cord torsion had no blood flow on ultrasound examination.", "contents": "The Doppler stethoscope and the diagnosis of the acute scrotum. We evaluated 30 patients who had acute scrotal swelling with the Doppler stethoscope. Every case of epididymitis and torsion of the appendix testis demonstrated an increased testicular blood flow. The cases of spermatic cord torsion had no blood flow on ultrasound examination.", "PMID": 978814}
{"id": "pubmed23n0032_11903", "title": "The effects of urine on the viability and activity of lymphocytes.", "content": "Circulating lymphocytes were incubated for 72 hours in either pure culture medium or a medium-urine mixture in the presence of phytohemagglutinin, concanavalin A or pokeweed mitogen, and assayed for blastogenesis and viability. The amount and osmolality of the urine were varied. Between 80 and 90 per cent of the cells remained viable during the entire study despite the culture mixture. If 50 percent of the culture was replaced by urine at an osmolality of 150, 300 or 500 mOsm, a severe but uniform depression of lymphocyte activity was seen. However, if only 25 percent or less of the medium was replaced by urine at 300 mOsm, the cells stimulated as well as they did in pure medium.", "contents": "The effects of urine on the viability and activity of lymphocytes. Circulating lymphocytes were incubated for 72 hours in either pure culture medium or a medium-urine mixture in the presence of phytohemagglutinin, concanavalin A or pokeweed mitogen, and assayed for blastogenesis and viability. The amount and osmolality of the urine were varied. Between 80 and 90 per cent of the cells remained viable during the entire study despite the culture mixture. If 50 percent of the culture was replaced by urine at an osmolality of 150, 300 or 500 mOsm, a severe but uniform depression of lymphocyte activity was seen. However, if only 25 percent or less of the medium was replaced by urine at 300 mOsm, the cells stimulated as well as they did in pure medium.", "PMID": 978815}
{"id": "pubmed23n0032_11904", "title": "Neuromuscular dysfunction of the lower urinary tract in patients with lesions of the cauda equina and conus medullaris.", "content": "Bladder and urethral function was studied in 21 patients with lesions of the cauda equina or conus medullaris using gas cystometry, integrated sphincter electromyography, uroflowmetry and computer assisted measurement of detrusor and urethral innervation. An areflexic cystometrogram, impaired electromyographic sphincter activity and delayed reflex-evoked potentials from stimulation of the detrusor muscle and urethra were the most consistent indicators of lesions of the conus medullaris and/oather than the ice water test is suggested.", "contents": "Neuromuscular dysfunction of the lower urinary tract in patients with lesions of the cauda equina and conus medullaris. Bladder and urethral function was studied in 21 patients with lesions of the cauda equina or conus medullaris using gas cystometry, integrated sphincter electromyography, uroflowmetry and computer assisted measurement of detrusor and urethral innervation. An areflexic cystometrogram, impaired electromyographic sphincter activity and delayed reflex-evoked potentials from stimulation of the detrusor muscle and urethra were the most consistent indicators of lesions of the conus medullaris and/oather than the ice water test is suggested.", "PMID": 978816}
{"id": "pubmed23n0032_11905", "title": "A catheter withdrawing apparatus for clinical urethral pressure profile studies.", "content": "An apparatus for withdrawing a urethral catheter at a constant speed is described. The motor and the puller assembly are separate components so that the puller assembly can be autoclaved. By matching pulling speed to recorder chart speed a reproducible representation of urethral length can be obtained without using a displacement transducer. The apparatus has been used clinically for measuring urethral pressure profiles.", "contents": "A catheter withdrawing apparatus for clinical urethral pressure profile studies. An apparatus for withdrawing a urethral catheter at a constant speed is described. The motor and the puller assembly are separate components so that the puller assembly can be autoclaved. By matching pulling speed to recorder chart speed a reproducible representation of urethral length can be obtained without using a displacement transducer. The apparatus has been used clinically for measuring urethral pressure profiles.", "PMID": 978817}
{"id": "pubmed23n0032_11906", "title": "Complications of vesicoureteral operations from incoordination of micturition.", "content": "Recently, a study was made of 8 boys with complications after corrective surgical procedures for dysfunction of the voiding mechanism. One of these patients already had been subjected to irreversible diversion before the basic disorder was recognized but the majority was treated with suggestion (often with hypnosis) as well as with drugs affecting the smooth muscle (1 adolescent was too hostile to accept treatment). Because the bladder was dysfunctional, the operations either failed or sequelae persisted until measures such as suggestion, hypnotherapy and retraining reversed the incoordination of micturition. Operation or reoperation in 5 children might not have been necessary if bladder coordination had been established earlier. The case of boys with poor urinary control but free of neurologic stigmas are suspect. If they require an operation for repair of congenital urinary defects such as reflux, equal attention must be paid to psychologic and habit patterns causing bladder dysfunction. Otherwise, the operation is done essentially into a neurogenic bladder. It is concluded that psychologically conditioned incoordination of voiding may alone produce mid and upper tract damage. The cases of boys with problems of urinary control not explained by neurologic or anatomic defects should be suspected and corrective surgical procedures may fail if bladder dysfunction is not corrected by retraining, suggestion and even hypnotherapy.", "contents": "Complications of vesicoureteral operations from incoordination of micturition. Recently, a study was made of 8 boys with complications after corrective surgical procedures for dysfunction of the voiding mechanism. One of these patients already had been subjected to irreversible diversion before the basic disorder was recognized but the majority was treated with suggestion (often with hypnosis) as well as with drugs affecting the smooth muscle (1 adolescent was too hostile to accept treatment). Because the bladder was dysfunctional, the operations either failed or sequelae persisted until measures such as suggestion, hypnotherapy and retraining reversed the incoordination of micturition. Operation or reoperation in 5 children might not have been necessary if bladder coordination had been established earlier. The case of boys with poor urinary control but free of neurologic stigmas are suspect. If they require an operation for repair of congenital urinary defects such as reflux, equal attention must be paid to psychologic and habit patterns causing bladder dysfunction. Otherwise, the operation is done essentially into a neurogenic bladder. It is concluded that psychologically conditioned incoordination of voiding may alone produce mid and upper tract damage. The cases of boys with problems of urinary control not explained by neurologic or anatomic defects should be suspected and corrective surgical procedures may fail if bladder dysfunction is not corrected by retraining, suggestion and even hypnotherapy.", "PMID": 978818}
{"id": "pubmed23n0032_11907", "title": "Calcified solitary renal cyst in childhood.", "content": "There is a 20% incidence of malignancy in renal masses with rim calcification in adults. A case of rim calcification in a solitary renal cyst in a child is presented and the diagnostic approach and surgical management are described. Solitary renal cysts are extremely rare in children, with no reported cases of rim calcification.", "contents": "Calcified solitary renal cyst in childhood. There is a 20% incidence of malignancy in renal masses with rim calcification in adults. A case of rim calcification in a solitary renal cyst in a child is presented and the diagnostic approach and surgical management are described. Solitary renal cysts are extremely rare in children, with no reported cases of rim calcification.", "PMID": 978819}
{"id": "pubmed23n0032_11908", "title": "Hypertension and post-traumatic renal arteriovenous fistula: demonstration of unilaterally elevated renin secretion.", "content": "Ipsilateral elevation and contralateral suppression of renin secretion have been established in a patient with a post-traumatic renal arteriovenous fistula associated with diastolic hypertension. This finding is rare because of the dilutional effect of arterial blood shunted in the renal vein. Increased renin secretion in this instance supports the proposed mechanism of diastolic hypertension with renal arteriovenous fistula.", "contents": "Hypertension and post-traumatic renal arteriovenous fistula: demonstration of unilaterally elevated renin secretion. Ipsilateral elevation and contralateral suppression of renin secretion have been established in a patient with a post-traumatic renal arteriovenous fistula associated with diastolic hypertension. This finding is rare because of the dilutional effect of arterial blood shunted in the renal vein. Increased renin secretion in this instance supports the proposed mechanism of diastolic hypertension with renal arteriovenous fistula.", "PMID": 978820}
{"id": "pubmed23n0032_11909", "title": "Anatomical variation of the renal veins with varicosity presenting as pseudotumor of the kidney.", "content": "Multiple renal veins opening separately into the inferior vena cava are rare, particularly in the left kidney. To my knowledge there have been no reports of this anatomical variation in the radiologic or urologic literature.", "contents": "Anatomical variation of the renal veins with varicosity presenting as pseudotumor of the kidney. Multiple renal veins opening separately into the inferior vena cava are rare, particularly in the left kidney. To my knowledge there have been no reports of this anatomical variation in the radiologic or urologic literature.", "PMID": 978821}
{"id": "pubmed23n0032_11910", "title": "Acute pyelonephritis simulating poorly vascularized renal neoplasm. Non-specificity of angiographic criteria.", "content": "A case of acute pyelonephritis associated with venous encasement and amputation is described. Although venous encasement and amputation have been considered previously as specific signs of malignancy, it is now clear that these signs are non-specific, occurring also in inflammatory diseases of the kidney.", "contents": "Acute pyelonephritis simulating poorly vascularized renal neoplasm. Non-specificity of angiographic criteria. A case of acute pyelonephritis associated with venous encasement and amputation is described. Although venous encasement and amputation have been considered previously as specific signs of malignancy, it is now clear that these signs are non-specific, occurring also in inflammatory diseases of the kidney.", "PMID": 978822}
{"id": "pubmed23n0032_11911", "title": "Inverted papilloma of the renal pelvis.", "content": "A case of an apparently benign urothelial tumor of the renal pelvis is reported. Morphologically, the lesion resembles an inverted papilloma. An article summarizing the findings in 28 of these tumors occurring in the bladder is quoted.", "contents": "Inverted papilloma of the renal pelvis. A case of an apparently benign urothelial tumor of the renal pelvis is reported. Morphologically, the lesion resembles an inverted papilloma. An article summarizing the findings in 28 of these tumors occurring in the bladder is quoted.", "PMID": 978824}
{"id": "pubmed23n0032_11912", "title": "Bilateral simulataneous kidney tumors of dissimilar cell type: a case report with emphasis on operative approach.", "content": "The first reported case of bilateral, simultaneous renal tumors of dissimilar cell type treated in a living patient is presented. The operative approach is outlined to support single in situ operative procedures in young patients. A second look laparotomy at 1 year and a creatinine clearance of 60 ml. per minute at 2 years substantiate our enthusiasm for this approach to bilateral renal lesions.", "contents": "Bilateral simulataneous kidney tumors of dissimilar cell type: a case report with emphasis on operative approach. The first reported case of bilateral, simultaneous renal tumors of dissimilar cell type treated in a living patient is presented. The operative approach is outlined to support single in situ operative procedures in young patients. A second look laparotomy at 1 year and a creatinine clearance of 60 ml. per minute at 2 years substantiate our enthusiasm for this approach to bilateral renal lesions.", "PMID": 978825}
{"id": "pubmed23n0032_11913", "title": "Filling defects in the renal pelvis and ureter owing to bleeding secondary to acquired circulating anticoagulants.", "content": "A case of transient filling defects in the renal pelves and ureters, secondary to spontaneously occurring acquired anticoagulants, is presented. The relationship of this entity to hemophilia, immunologic disorders, such as systemic lupus erythematosis, and normal post partum patients is discussed. The differential diagnosis for filling defects in the renal pelvis is reviewed.", "contents": "Filling defects in the renal pelvis and ureter owing to bleeding secondary to acquired circulating anticoagulants. A case of transient filling defects in the renal pelves and ureters, secondary to spontaneously occurring acquired anticoagulants, is presented. The relationship of this entity to hemophilia, immunologic disorders, such as systemic lupus erythematosis, and normal post partum patients is discussed. The differential diagnosis for filling defects in the renal pelvis is reviewed.", "PMID": 978826}
{"id": "pubmed23n0032_11914", "title": "Ureteral obstruction owing to endometriosis: reversal with synthetic progestin.", "content": "A case of unilateral ureteral obstruction owing to extensive pelvic endometriosis is presented. There was complete resolution of ureteral obstruction following treatment with synthetic progestin. Sequential assessment of renal function was provided by renal imaging and renograms. It would appear that selected patients with obstructive uropathy may respond favorably to medical management, which would allow for preservation of reproductive capabilities and restoration of renal excretory function.", "contents": "Ureteral obstruction owing to endometriosis: reversal with synthetic progestin. A case of unilateral ureteral obstruction owing to extensive pelvic endometriosis is presented. There was complete resolution of ureteral obstruction following treatment with synthetic progestin. Sequential assessment of renal function was provided by renal imaging and renograms. It would appear that selected patients with obstructive uropathy may respond favorably to medical management, which would allow for preservation of reproductive capabilities and restoration of renal excretory function.", "PMID": 978828}
{"id": "pubmed23n0032_11915", "title": "Epispadias associated with phimosis.", "content": "proximal to the coronal sulcus-proximal to the cornarl sulcus", "contents": "Epispadias associated with phimosis. proximal to the coronal sulcus-proximal to the cornarl sulcus", "PMID": 978830}
{"id": "pubmed23n0032_11916", "title": "Torsion of scrotal testis in a persistent processus vaginalis.", "content": "A case of an unusual association between hernia and testicular infarction, namely torsion of a normally descended testis within a persistent processus vaginalis, is presented. The advantages of the inguinal ingulinal approach for the exploration of suspected torsion are discussed. Although an operation was performed more than 30 hours after the onset of symptoms the testicle was viable and recovered.", "contents": "Torsion of scrotal testis in a persistent processus vaginalis. A case of an unusual association between hernia and testicular infarction, namely torsion of a normally descended testis within a persistent processus vaginalis, is presented. The advantages of the inguinal ingulinal approach for the exploration of suspected torsion are discussed. Although an operation was performed more than 30 hours after the onset of symptoms the testicle was viable and recovered.", "PMID": 978831}
{"id": "pubmed23n0032_11917", "title": "Klinefelter's syndrome and extragenital seminoma.", "content": "An extragenital metastatic seminoma in a patient with Klinefelter's syndrome is described. The increased incidence of many types of malignancies in patients with this chromosomal abnormality and the increased susceptibility of XXY cells to transformation by oncogenic SV-40 virus are discussed. A relationship is suggested circumstantially by these observations.", "contents": "Klinefelter's syndrome and extragenital seminoma. An extragenital metastatic seminoma in a patient with Klinefelter's syndrome is described. The increased incidence of many types of malignancies in patients with this chromosomal abnormality and the increased susceptibility of XXY cells to transformation by oncogenic SV-40 virus are discussed. A relationship is suggested circumstantially by these observations.", "PMID": 978832}
{"id": "pubmed23n0032_11918", "title": "Hemangioma of the testis in an infant.", "content": "Testicular tumors in infants and children are rare and most of them are malignant. Embryonal carcinoma is the most common childhood neoplasm, while seminoma is predominantly found in adults. Hemangioma of the testis is an extremely rare tumor, only 2 cases having been reported in infants.", "contents": "Hemangioma of the testis in an infant. Testicular tumors in infants and children are rare and most of them are malignant. Embryonal carcinoma is the most common childhood neoplasm, while seminoma is predominantly found in adults. Hemangioma of the testis is an extremely rare tumor, only 2 cases having been reported in infants.", "PMID": 978833}
{"id": "pubmed23n0032_11919", "title": "A new biological assay method for histamine-sensitizing factor using survival time as a response.", "content": "A new biological assay method using survival time of mice as a graded response after histamine challenge has been developed for the histamine-sensitizing factor of pertussis bacilli. The method is relatively precise and reproducible in estimation and fairly sensitive in the validity tests of biological assay. Effect of endotoxin on the estimation by the proposed method was also investigated in case the method is applied to the control test of pertussis vaccine.", "contents": "A new biological assay method for histamine-sensitizing factor using survival time as a response. A new biological assay method using survival time of mice as a graded response after histamine challenge has been developed for the histamine-sensitizing factor of pertussis bacilli. The method is relatively precise and reproducible in estimation and fairly sensitive in the validity tests of biological assay. Effect of endotoxin on the estimation by the proposed method was also investigated in case the method is applied to the control test of pertussis vaccine.", "PMID": 978834}
{"id": "pubmed23n0032_11920", "title": "Studies on delayed-type hypersensitivity to hen egg-white lysozyme. I. Peptide fragments of lysozyme inducing delayed-type hypersensitivity.", "content": "Eleven peaks were separated by Carboxymethyl-cellulose column chromatography of peptic digest of lysozyme. Being stronger in antigenic activity two peaks of them, P-3 and P-9, were selected and purified further respectively by Amberlite IRC-50 and Sephadex G-50 column chromatography. As the results, PP-3 and PP-9 were obtained each as a single peak. For estimation of their capacities to induce delayed-type hypersensitivity, the antigen-induced 3H-thymidine incorporation, the migration inhibition of peritoneal cells and the delayed-type skin reaction were tested in guinea pigs immunized with native lysozyme or any of its fractions. PP-9 was almost as active as intact lysozyme in these capacities. On the other hand, PP-3 showed a slight inhibition of migration of peritoneal cells and no stimulation of 3H-thymidine incorporation into the lymph node cells. Moreover, the delayed-type skin reaction elicited by PP-3 was always weaker than that elicited by PP-9. Guinea pigs immunized with either PP-3 or PP-9 were also tested for these reactions. PP-9 and native lysozyme elicited these reactions in guinea pigs immunized with PP-9, but PP-3 did not. On the other hand, PP-3 and lysozyme elicited these reactions in those immunized with PP-3, but PP-9 did not. The possibility of recognition of two functionally different areas, one for production of the circulating antibody and the other for induction of delayed-type hypersensitivity, on the lysozyme molecule was discussed.", "contents": "Studies on delayed-type hypersensitivity to hen egg-white lysozyme. I. Peptide fragments of lysozyme inducing delayed-type hypersensitivity. Eleven peaks were separated by Carboxymethyl-cellulose column chromatography of peptic digest of lysozyme. Being stronger in antigenic activity two peaks of them, P-3 and P-9, were selected and purified further respectively by Amberlite IRC-50 and Sephadex G-50 column chromatography. As the results, PP-3 and PP-9 were obtained each as a single peak. For estimation of their capacities to induce delayed-type hypersensitivity, the antigen-induced 3H-thymidine incorporation, the migration inhibition of peritoneal cells and the delayed-type skin reaction were tested in guinea pigs immunized with native lysozyme or any of its fractions. PP-9 was almost as active as intact lysozyme in these capacities. On the other hand, PP-3 showed a slight inhibition of migration of peritoneal cells and no stimulation of 3H-thymidine incorporation into the lymph node cells. Moreover, the delayed-type skin reaction elicited by PP-3 was always weaker than that elicited by PP-9. Guinea pigs immunized with either PP-3 or PP-9 were also tested for these reactions. PP-9 and native lysozyme elicited these reactions in guinea pigs immunized with PP-9, but PP-3 did not. On the other hand, PP-3 and lysozyme elicited these reactions in those immunized with PP-3, but PP-9 did not. The possibility of recognition of two functionally different areas, one for production of the circulating antibody and the other for induction of delayed-type hypersensitivity, on the lysozyme molecule was discussed.", "PMID": 978835}
{"id": "pubmed23n0032_11921", "title": "Biological activities of endotoxins from Yersinia enterocolitica.", "content": "The chemical properties and the general biological activities of lipopolysaccharide (LPS) and Boivin-type endotoxin obtained respectively by phenol-water and trichloroacetic acid extraction from Yersinia enterocolitica serotypes O3 and O9 were studied. The yield of LPS from the O9 strain was about 10% of the O3 strain possibly because of the lower solubility of O9-LPS in aqueous phase. However, the chemical composition of O9-LPS was similar to that of O3-LPS in the proportions of reducing sugar, glucosamine, heptose, KDO, and lipid A. In pyrogenicity and local Shwartzman reactivity in rabbits and lethality for mice, there was also no difference between O3 and O9-LPS. The anthrone-positive carbohydrate and lipid A contents of Boivin-type endotoxin from O3 were higher than those of the endotoxin from O9. The biological activities of Boivin-type endotoxin from O3 were also remarkably higher than those of the endotoxin from O9. It seems that endotoxin of Y. enterocolitica serotype O3 may play an important role in infection by this organism.", "contents": "Biological activities of endotoxins from Yersinia enterocolitica. The chemical properties and the general biological activities of lipopolysaccharide (LPS) and Boivin-type endotoxin obtained respectively by phenol-water and trichloroacetic acid extraction from Yersinia enterocolitica serotypes O3 and O9 were studied. The yield of LPS from the O9 strain was about 10% of the O3 strain possibly because of the lower solubility of O9-LPS in aqueous phase. However, the chemical composition of O9-LPS was similar to that of O3-LPS in the proportions of reducing sugar, glucosamine, heptose, KDO, and lipid A. In pyrogenicity and local Shwartzman reactivity in rabbits and lethality for mice, there was also no difference between O3 and O9-LPS. The anthrone-positive carbohydrate and lipid A contents of Boivin-type endotoxin from O3 were higher than those of the endotoxin from O9. The biological activities of Boivin-type endotoxin from O3 were also remarkably higher than those of the endotoxin from O9. It seems that endotoxin of Y. enterocolitica serotype O3 may play an important role in infection by this organism.", "PMID": 978837}
{"id": "pubmed23n0032_11922", "title": "Tolerance and other biological properties of Vibrio parahaemolyticus endotoxins.", "content": "Biological properties of endotoxins prepared from three strains of Vibrio parahaemolyticus were compared with reference to commercially prepared Salmonella typhi endotoxin. Endotoxin assays performed in rabbits included dermal Shwartzman reactivity, pyrogenicity, heat stability, and ability to induce tolerance as well as cross-tolerance. Mice were used for endotoxin LD50 determinations. Results showed V. parahaemolyticus endotoxins were similar to that of S. typhi strain O901. Induction of tolerance to V. parahaemolyticus strain 11590 endotoxin resulted in complete cross-tolerance to S. typhi endotoxin, and vice versa. Partial cross-tolerance to S. typhi endotoxin was demonstrated with rabbits rendered tolerant to endotoxin from V. parahaemolyticus strains Sak-3 and FC1011. Absorption spectra, nitrogen, phosphorus and carbohydrate analyses revealed additional similarities between endotoxins from V. parahaemolyticus and endotoxin from a member of the Enterbacteriaceae.", "contents": "Tolerance and other biological properties of Vibrio parahaemolyticus endotoxins. Biological properties of endotoxins prepared from three strains of Vibrio parahaemolyticus were compared with reference to commercially prepared Salmonella typhi endotoxin. Endotoxin assays performed in rabbits included dermal Shwartzman reactivity, pyrogenicity, heat stability, and ability to induce tolerance as well as cross-tolerance. Mice were used for endotoxin LD50 determinations. Results showed V. parahaemolyticus endotoxins were similar to that of S. typhi strain O901. Induction of tolerance to V. parahaemolyticus strain 11590 endotoxin resulted in complete cross-tolerance to S. typhi endotoxin, and vice versa. Partial cross-tolerance to S. typhi endotoxin was demonstrated with rabbits rendered tolerant to endotoxin from V. parahaemolyticus strains Sak-3 and FC1011. Absorption spectra, nitrogen, phosphorus and carbohydrate analyses revealed additional similarities between endotoxins from V. parahaemolyticus and endotoxin from a member of the Enterbacteriaceae.", "PMID": 978838}
{"id": "pubmed23n0032_11923", "title": "Inhibition of rat dental caries by dextranase from a strain of Spicaria violacea.", "content": "Dextranase AD17 obtained from a culture liquor of a strain of Spicaria violacea was assessed for its ability to inhibit the development of dental caries in conventional Sprague-Dawley rats which had been infected with one of the Streptococcus mutans strains. MT6R (serotype c), OMZ 176R (d), or MT-703R (e). These experiments showed that caries was significantly inhibited when rats were given cariogenic diets No. 2000 and drinking water containing AD17 at a concentration of 10 units/g, as compared to control rats not given dextranase. The inhibitory effects of AD17 were more prominent in smooth surface caries than in total caries. AD17 had a tendency to retard both the establishment of inoculated S. mutans and plaque deposition on tooth surfaces. However, S. mutans could be implanted in the rat oral cavity after repeated inoculation of the bacteria, even in the presence of AD17. These results suggest that the anticaries activity of AD17 is due to not only inhibition of adherence of S. mutans cells on tooth surfaces but also to physiochemical changes of dental plaque formed under the enzymatic action of AD17. Preliminary histophatological examination showed that AD17 had no significant toxicity in rats.", "contents": "Inhibition of rat dental caries by dextranase from a strain of Spicaria violacea. Dextranase AD17 obtained from a culture liquor of a strain of Spicaria violacea was assessed for its ability to inhibit the development of dental caries in conventional Sprague-Dawley rats which had been infected with one of the Streptococcus mutans strains. MT6R (serotype c), OMZ 176R (d), or MT-703R (e). These experiments showed that caries was significantly inhibited when rats were given cariogenic diets No. 2000 and drinking water containing AD17 at a concentration of 10 units/g, as compared to control rats not given dextranase. The inhibitory effects of AD17 were more prominent in smooth surface caries than in total caries. AD17 had a tendency to retard both the establishment of inoculated S. mutans and plaque deposition on tooth surfaces. However, S. mutans could be implanted in the rat oral cavity after repeated inoculation of the bacteria, even in the presence of AD17. These results suggest that the anticaries activity of AD17 is due to not only inhibition of adherence of S. mutans cells on tooth surfaces but also to physiochemical changes of dental plaque formed under the enzymatic action of AD17. Preliminary histophatological examination showed that AD17 had no significant toxicity in rats.", "PMID": 978839}
{"id": "pubmed23n0032_11924", "title": "Attainment and stability of the performance in differential low rate water reinforcement in rats.", "content": "The establishment and stability of the behavioral baseline for rats in relation to the schedule of differential reinforcement of low rate under water reinforcer (DRL 20 sec for water) were studied, with the following results: When the DRL value was gradually stepped up from 1 sec to 20 sec with the advance of the sessions from 1to 16, the establishment of the behavioral baseline was slower than when DRL 20 sec was applied from the start. The establishment of the baseline was clearly accelerated by the prolongation of the length of time for training in one session from 60 min to 120 min. The baseline remained highly stable without being affected by the intermittent administration (2-3 times a week) of methamphetamine and diazepam, each in doses from 0.06 to 1.0 mg/kg, and of caffeine and pentobarbital, each in doses from 1.2 to 20 mg/kg, or by the discontinuation of the test from 1 to 15 days. However, during the retraining period following the test discontinuation it was found that the baseline fluctuated for a long time due to the elimination of water deprivation. The baseline stability, once established, could be maintained through about 300 daily sessions, with only a slight dependence on the change in environmental conditions such as humidity, temperature, and the season.", "contents": "Attainment and stability of the performance in differential low rate water reinforcement in rats. The establishment and stability of the behavioral baseline for rats in relation to the schedule of differential reinforcement of low rate under water reinforcer (DRL 20 sec for water) were studied, with the following results: When the DRL value was gradually stepped up from 1 sec to 20 sec with the advance of the sessions from 1to 16, the establishment of the behavioral baseline was slower than when DRL 20 sec was applied from the start. The establishment of the baseline was clearly accelerated by the prolongation of the length of time for training in one session from 60 min to 120 min. The baseline remained highly stable without being affected by the intermittent administration (2-3 times a week) of methamphetamine and diazepam, each in doses from 0.06 to 1.0 mg/kg, and of caffeine and pentobarbital, each in doses from 1.2 to 20 mg/kg, or by the discontinuation of the test from 1 to 15 days. However, during the retraining period following the test discontinuation it was found that the baseline fluctuated for a long time due to the elimination of water deprivation. The baseline stability, once established, could be maintained through about 300 daily sessions, with only a slight dependence on the change in environmental conditions such as humidity, temperature, and the season.", "PMID": 978843}
{"id": "pubmed23n0032_11925", "title": "Fentanyl and tooth pulp evoked responses in the spinal trigeminal nucleus caudalis region.", "content": "Electrical activities evoked by tooth pulp stimulation were recorded in the subnucleus caudalis region of the spinal trigeminal nucleus, and the action of fentanyl, a short-acting narcotic, on these activities was investigated in the alpha-cholinergic anesthetized cat. Fentanyl (20 to 40 mug/kg i.v.) depressed the first peak and potentiated the second one of negative potentials evoked by pulp stimulation in the border area betaeen the nucleus proprius (Pr) and the lateral reticular formation (LRF). Neurons, whose responses to pulp stimulation were depressed by fentanyl, were also predominantly localized in this region. Pulp-induced monophasic negative potential and spike discharges in the more ventro-medial portion of the LRF were not affected by fentanyl. The effect of fentanyl on cells in the marginal zone varied from unit to unit. The selective action of fentanyl on neurons in the border area between the Pr and the LRF may partially explain the analgesic action of fentanyl.", "contents": "Fentanyl and tooth pulp evoked responses in the spinal trigeminal nucleus caudalis region. Electrical activities evoked by tooth pulp stimulation were recorded in the subnucleus caudalis region of the spinal trigeminal nucleus, and the action of fentanyl, a short-acting narcotic, on these activities was investigated in the alpha-cholinergic anesthetized cat. Fentanyl (20 to 40 mug/kg i.v.) depressed the first peak and potentiated the second one of negative potentials evoked by pulp stimulation in the border area betaeen the nucleus proprius (Pr) and the lateral reticular formation (LRF). Neurons, whose responses to pulp stimulation were depressed by fentanyl, were also predominantly localized in this region. Pulp-induced monophasic negative potential and spike discharges in the more ventro-medial portion of the LRF were not affected by fentanyl. The effect of fentanyl on cells in the marginal zone varied from unit to unit. The selective action of fentanyl on neurons in the border area between the Pr and the LRF may partially explain the analgesic action of fentanyl.", "PMID": 978844}
{"id": "pubmed23n0032_11926", "title": "Inhibition of thalamic and hypothalamic somatosensory evoked potentials by stimulation of substantia nigra and its modification by morphine and methotrimeprazine (levomepromazine).", "content": "A brief electrical stimulation of the substantia nigra induced a marked and long lasting inhibition of the somatosensory evoked potentials recorded from the centrum medianum of the thalamus (CM) and posterior hypothalamic area (PHA) following sciatic stimulation in unanesthetized rabbits. The nigral inhibitory effect on CM was prolonged by the administration of morphine (4 mg/kg i.v.) but not influenced by that of methotrimeprazine (2-4 mg/kg i.v.). In contrast, the nigral inhibitory effect on PHA was enhanced by the injection of methotrimeprazine (2 mg/kg i.v.), but not changed by that of morphine (4 mg/kg i.v.). These results indicate that the inhibitory system originating from the substantia nigra operates on the somatosensory transmissions from the peripheral nerve to the thalamus and hypothalamus, and that morphine or methotrimeprazine in small doses induced a selective potentiation of the nigral inhibitory influence on the thalamus or hypothalamus, respectively.", "contents": "Inhibition of thalamic and hypothalamic somatosensory evoked potentials by stimulation of substantia nigra and its modification by morphine and methotrimeprazine (levomepromazine). A brief electrical stimulation of the substantia nigra induced a marked and long lasting inhibition of the somatosensory evoked potentials recorded from the centrum medianum of the thalamus (CM) and posterior hypothalamic area (PHA) following sciatic stimulation in unanesthetized rabbits. The nigral inhibitory effect on CM was prolonged by the administration of morphine (4 mg/kg i.v.) but not influenced by that of methotrimeprazine (2-4 mg/kg i.v.). In contrast, the nigral inhibitory effect on PHA was enhanced by the injection of methotrimeprazine (2 mg/kg i.v.), but not changed by that of morphine (4 mg/kg i.v.). These results indicate that the inhibitory system originating from the substantia nigra operates on the somatosensory transmissions from the peripheral nerve to the thalamus and hypothalamus, and that morphine or methotrimeprazine in small doses induced a selective potentiation of the nigral inhibitory influence on the thalamus or hypothalamus, respectively.", "PMID": 978846}
{"id": "pubmed23n0032_11927", "title": "Effects of indomethacin and the other anti-inflammatory agents on activation of dorsal horn cell in the spinal cord induced by intra-arterial injection of bradykinin.", "content": "Bradykinin (1-2 mug) injected into the femoral artery of the rabbit induced a marked increase in the firing rates of the dorsal horn cells (lamina V cells). This response was markedly inhibited by intravenous administration of indomethacin (1.0 mg/kg), acetylsalicyclic acid (20 mg/kg), aminopyrine (50 mg/kg) and oxyphenbutazone (20 mg/kg) in both intact and spinal preparations. Moreover, the intra-arterial injection of these agents depressed the response in lower doses. With these same low doses, no significant suppression occurred when the intravenous route was used. These results indicate that indomethacin and other anti-inflammatory agents tested suppress the bradykinin-induced response of the dorsal horn cell at the periphery, probably at the paravascular sensory nerve.", "contents": "Effects of indomethacin and the other anti-inflammatory agents on activation of dorsal horn cell in the spinal cord induced by intra-arterial injection of bradykinin. Bradykinin (1-2 mug) injected into the femoral artery of the rabbit induced a marked increase in the firing rates of the dorsal horn cells (lamina V cells). This response was markedly inhibited by intravenous administration of indomethacin (1.0 mg/kg), acetylsalicyclic acid (20 mg/kg), aminopyrine (50 mg/kg) and oxyphenbutazone (20 mg/kg) in both intact and spinal preparations. Moreover, the intra-arterial injection of these agents depressed the response in lower doses. With these same low doses, no significant suppression occurred when the intravenous route was used. These results indicate that indomethacin and other anti-inflammatory agents tested suppress the bradykinin-induced response of the dorsal horn cell at the periphery, probably at the paravascular sensory nerve.", "PMID": 978845}
{"id": "pubmed23n0032_11928", "title": "Centrally mediated cardiovascular effects of intracisternal application of carbachol in anesthetized rats.", "content": "The pressor response to the intracisternal (i.c.) injection of carbachol (1 mug) in anesthetized rats was analyzed. This response was significantly reduced by the intravenous (i.v.) injection of guanethidine (5 mg), hexamethonium (10 mg) or phentolamine (5 mg), and conversely, potentiated by i.v. desmethylimipramine (0.3 mg), while propranolol (0.5 mg) i.v. selectively inhibited the enlargement of pulse pressure and the tachycardia following i.c. carbachol (1 mug). On the other hand, the pressor response to i.c. carbachol (1 mug) was almost completely blocked by i.c. atropine (3 mug) or hexamethonium (500 mug), and significantly reduced by i.c. chlorpromazine (50 mug) but significantly potentiated by i.c. desmethylimipramine (30 mug). The pressor response to i.c. carbachol (1 mug) remained unchanged after sectioning of the bilateral cervical vagal nerves but disappeared after sectioning of the spinal cord (C7-C8). From the above result it is suggested that the pressor response to i.c. carbachol ortral and peripheral adrenergic mechanisms, and that the sympathetic trunk is the main pathway.", "contents": "Centrally mediated cardiovascular effects of intracisternal application of carbachol in anesthetized rats. The pressor response to the intracisternal (i.c.) injection of carbachol (1 mug) in anesthetized rats was analyzed. This response was significantly reduced by the intravenous (i.v.) injection of guanethidine (5 mg), hexamethonium (10 mg) or phentolamine (5 mg), and conversely, potentiated by i.v. desmethylimipramine (0.3 mg), while propranolol (0.5 mg) i.v. selectively inhibited the enlargement of pulse pressure and the tachycardia following i.c. carbachol (1 mug). On the other hand, the pressor response to i.c. carbachol (1 mug) was almost completely blocked by i.c. atropine (3 mug) or hexamethonium (500 mug), and significantly reduced by i.c. chlorpromazine (50 mug) but significantly potentiated by i.c. desmethylimipramine (30 mug). The pressor response to i.c. carbachol (1 mug) remained unchanged after sectioning of the bilateral cervical vagal nerves but disappeared after sectioning of the spinal cord (C7-C8). From the above result it is suggested that the pressor response to i.c. carbachol ortral and peripheral adrenergic mechanisms, and that the sympathetic trunk is the main pathway.", "PMID": 978847}
{"id": "pubmed23n0032_11929", "title": "Effect of anoxia on isotonic shortening induced by high-K medium in rat uterus.", "content": "The effect of anoxia on the shortening induced by high-K medium was investigated in isolated rat uterus. The shortening induced by high-K medium was maintained for 75 min or more under normoxia in the initial load (0.35 g). Under normoxia 1, 2, 2.5, and 3 g load to the uterus reduced the maximum shortening induced by high-K medium to 93, 65, 18 and 3%, respectively. Under anoxia the high-K-induced shortening in the initial load was not so affected and it was maintained at least for 60 min. However, against 0.7 and 1 g load to the uterus, the maximum shortening reduced greatly to 14 and 13% under anoxia. Effects of anoxia on the shortenings induced by high-K medium in guinea pigs taenia coli and longitudinal muscle from ileum were also studied. Our observations support the theory that the shortening of the light loaded muscle under anoxia is maintained by only a little force and the muscle may not be so dependent on metabolic energy.", "contents": "Effect of anoxia on isotonic shortening induced by high-K medium in rat uterus. The effect of anoxia on the shortening induced by high-K medium was investigated in isolated rat uterus. The shortening induced by high-K medium was maintained for 75 min or more under normoxia in the initial load (0.35 g). Under normoxia 1, 2, 2.5, and 3 g load to the uterus reduced the maximum shortening induced by high-K medium to 93, 65, 18 and 3%, respectively. Under anoxia the high-K-induced shortening in the initial load was not so affected and it was maintained at least for 60 min. However, against 0.7 and 1 g load to the uterus, the maximum shortening reduced greatly to 14 and 13% under anoxia. Effects of anoxia on the shortenings induced by high-K medium in guinea pigs taenia coli and longitudinal muscle from ileum were also studied. Our observations support the theory that the shortening of the light loaded muscle under anoxia is maintained by only a little force and the muscle may not be so dependent on metabolic energy.", "PMID": 978848}
{"id": "pubmed23n0032_11930", "title": "Age-dependence of the chronotropic response to noradrenaline, acetylcholine and transmural stimulation in isolated rabbit atria.", "content": "The chonotropic response to noradrenaline, tyramine, acetylcholine and transmural electrical stimulation was compared in atria isolated from rabbits at different stages of developmental after birth (day 2 to day 210). Pacemaker rates understeady state conditions were related inversely to days after birth; the rate in atria from rabbits at day 2 was significantly greater than that at days 10-210. The rate of neonatal rabbit atria was not significantly reduced by propranolol and the positive chontropic response to noradrenaline was not significantly reduced by propranolol and the positive chronotropic response to noradrenaline was not significantly different in atria from ages of rabbits as far as threshold concentrations for inducing tachycardia and ED50's were concerned. The maximum rate induced by noradrenaline was higher in neonatal rabbit atria than in adult rabbit atria. The effect of tyramine was approx. the same regardless of age. Increase in the pacemaker rate induced by transmural neural stimulation varied directly with age. The negative chronotropic effect of acetylcholine was greater in neonatal than in adult rabbit atria; the ED50 in the former was significantly less than in the latter. Bradycardia induced by transmural stimulation of intracardiac cholinergic nerves was related directly to age. Tachycardia in the neonatal rabbit atria may be due to electrogenic characteristics of pacemaker cells which differ from those in adult rabbit atria. Our evidence strongly suggests that the adrenergic and cholinergic nerves innervating the S-A node develop at an early postnatal stage in the rabbit.", "contents": "Age-dependence of the chronotropic response to noradrenaline, acetylcholine and transmural stimulation in isolated rabbit atria. The chonotropic response to noradrenaline, tyramine, acetylcholine and transmural electrical stimulation was compared in atria isolated from rabbits at different stages of developmental after birth (day 2 to day 210). Pacemaker rates understeady state conditions were related inversely to days after birth; the rate in atria from rabbits at day 2 was significantly greater than that at days 10-210. The rate of neonatal rabbit atria was not significantly reduced by propranolol and the positive chontropic response to noradrenaline was not significantly reduced by propranolol and the positive chronotropic response to noradrenaline was not significantly different in atria from ages of rabbits as far as threshold concentrations for inducing tachycardia and ED50's were concerned. The maximum rate induced by noradrenaline was higher in neonatal rabbit atria than in adult rabbit atria. The effect of tyramine was approx. the same regardless of age. Increase in the pacemaker rate induced by transmural neural stimulation varied directly with age. The negative chronotropic effect of acetylcholine was greater in neonatal than in adult rabbit atria; the ED50 in the former was significantly less than in the latter. Bradycardia induced by transmural stimulation of intracardiac cholinergic nerves was related directly to age. Tachycardia in the neonatal rabbit atria may be due to electrogenic characteristics of pacemaker cells which differ from those in adult rabbit atria. Our evidence strongly suggests that the adrenergic and cholinergic nerves innervating the S-A node develop at an early postnatal stage in the rabbit.", "PMID": 978849}
{"id": "pubmed23n0032_11931", "title": "A new guanidine derivative: dissociation of the adrenergic neuron blocking activity from local anesthetic activity.", "content": "Effects of 4-7-exo-methylene-hexahydroisoindoline-ethyl-guanidine hemisulfate (No. 865-123), a new guanidine derivative, on adrenergic neurons and its local anesthetic activity were investigated in comparison with guanethidine. Both derivatives produced in a dose-dependent manner a progressive irreversible reduction of positive chronotropic and contractile responses to postganglionic sympathetic nerve stimulation in isolated rabbit atria. The time course of the reduction by No. 865-123 was somewhat slower. In dogs administered both agents, the pressor response to carotid occlusion was reduced and that to exogenous noradrenaline was potentiated with a slight decrease in blood pressure. Noradrenaline stores in the heart and spleen of rats were also depleted to a similar extent. In the guinea pig weal method, guanethidine acted as a potent local anesthetic with a slow onset and a prolonged action as compared to procaine. No. 865-123 revealed no more anesthetic activity than did saline. It is unlikely that the local anesthetic activity of the guanidine derivatives contributes to the adrenergic neuron blocking activity.", "contents": "A new guanidine derivative: dissociation of the adrenergic neuron blocking activity from local anesthetic activity. Effects of 4-7-exo-methylene-hexahydroisoindoline-ethyl-guanidine hemisulfate (No. 865-123), a new guanidine derivative, on adrenergic neurons and its local anesthetic activity were investigated in comparison with guanethidine. Both derivatives produced in a dose-dependent manner a progressive irreversible reduction of positive chronotropic and contractile responses to postganglionic sympathetic nerve stimulation in isolated rabbit atria. The time course of the reduction by No. 865-123 was somewhat slower. In dogs administered both agents, the pressor response to carotid occlusion was reduced and that to exogenous noradrenaline was potentiated with a slight decrease in blood pressure. Noradrenaline stores in the heart and spleen of rats were also depleted to a similar extent. In the guinea pig weal method, guanethidine acted as a potent local anesthetic with a slow onset and a prolonged action as compared to procaine. No. 865-123 revealed no more anesthetic activity than did saline. It is unlikely that the local anesthetic activity of the guanidine derivatives contributes to the adrenergic neuron blocking activity.", "PMID": 978850}
{"id": "pubmed23n0032_11932", "title": "Clinical characteristics of the carcinoma of the stomach and its localization.", "content": "The result of follow-up study and the localization of gastric carcinoma subjected to the operation at the Second Department of Surgery, Faculty of Medicine, Kyushu University during 7 year period from 1963 to 1969 form the basis of the present communication. The curative resectability was 70.3 per cent in the carcinoma of the distal third of the stomach (A), 82.9 per cent in that of the middle third (M) and 55.3 in that of the proximal third (C); the 5-year survival rate was 48.6 per cent in the cancer of A, 81.1 per cent in that of M and 49.1 per cent in that of C respectively. In the carcinoma of A incidence of high grade penetrative invasion into the gastric wall as well as metastatic involvment was high. It is noteworthy that the tendency of the metastasis was found even in those with low grade penetration. In the carcinoma of M the incidence of high grade penetration as well as lymphatic metastasis was relatively low. The hepatic metastasis and peritoneal dissemination was also not frequent. In the carcinoma of C the incidence of high grade penetration was very high and the lymphatic metastasis was frequently observed in those with high grade penetration. Biological characteristics of the carcinoma of the stomach in each location and their therapeutic significance are discussed.", "contents": "Clinical characteristics of the carcinoma of the stomach and its localization. The result of follow-up study and the localization of gastric carcinoma subjected to the operation at the Second Department of Surgery, Faculty of Medicine, Kyushu University during 7 year period from 1963 to 1969 form the basis of the present communication. The curative resectability was 70.3 per cent in the carcinoma of the distal third of the stomach (A), 82.9 per cent in that of the middle third (M) and 55.3 in that of the proximal third (C); the 5-year survival rate was 48.6 per cent in the cancer of A, 81.1 per cent in that of M and 49.1 per cent in that of C respectively. In the carcinoma of A incidence of high grade penetrative invasion into the gastric wall as well as metastatic involvment was high. It is noteworthy that the tendency of the metastasis was found even in those with low grade penetration. In the carcinoma of M the incidence of high grade penetration as well as lymphatic metastasis was relatively low. The hepatic metastasis and peritoneal dissemination was also not frequent. In the carcinoma of C the incidence of high grade penetration was very high and the lymphatic metastasis was frequently observed in those with high grade penetration. Biological characteristics of the carcinoma of the stomach in each location and their therapeutic significance are discussed.", "PMID": 978854}
{"id": "pubmed23n0032_11933", "title": "The effect of cartilage-bone marrow extract (CBME) on the healing of intestinal anastomosis.", "content": "The intestinal anastomosis made under various conditions with and without treatment with cartilage-bone marrow extract were compared. The preoperative or postoperative administration of cartilage-bone marrow extract was found to increase mechanical strength of the intestinal anastomosis, even under unfavorable conditions such as hypoproteinemia, long term treatment of corticoid or reanastomosis. Formation of connective tissue and healing process were also found to be promoted by cartilage-bone marrow extract treatment.", "contents": "The effect of cartilage-bone marrow extract (CBME) on the healing of intestinal anastomosis. The intestinal anastomosis made under various conditions with and without treatment with cartilage-bone marrow extract were compared. The preoperative or postoperative administration of cartilage-bone marrow extract was found to increase mechanical strength of the intestinal anastomosis, even under unfavorable conditions such as hypoproteinemia, long term treatment of corticoid or reanastomosis. Formation of connective tissue and healing process were also found to be promoted by cartilage-bone marrow extract treatment.", "PMID": 978856}
{"id": "pubmed23n0032_11934", "title": "Abdominal aneurysm with aortoduodenal fistula.", "content": "Two patients having the abdominal aneurysm with aortoduodenal fistula were treated surgically, but graft infection occurred. One patient died about 6 months after and the other about one year after the operation due to massive hemorrhage from the postoperative aortointestinal fistula. The difficulties in the treatment of the aortoduodenal fistula were discussed.", "contents": "Abdominal aneurysm with aortoduodenal fistula. Two patients having the abdominal aneurysm with aortoduodenal fistula were treated surgically, but graft infection occurred. One patient died about 6 months after and the other about one year after the operation due to massive hemorrhage from the postoperative aortointestinal fistula. The difficulties in the treatment of the aortoduodenal fistula were discussed.", "PMID": 978855}
{"id": "pubmed23n0032_11935", "title": "Reactive hyperplasia of paracortex and germinal centers of the regional lymph nodes in gastric carcinoma cases as a favorable prognostic indicator.", "content": "The prognosis of 141 patients subjected to curative resection for gastric carcinoma was analyzed with special reference to the paracortex, germinal centers and sinus histiocytes in the regional lymph nodes. Five years survival rates of the patients with paracortical and/or germinal center hyperplasia (PH and/or GH) was definitely superior to those without such hyperplasia. This difference was most pronounced in those with moderately advanced stage. The incidence of lymph node metastasis was lower in the patients with PH or GH. The presence of sinus histiocytosis (SH) seemed to be correlated neither with a longer survival nor with lower incidence of lymph node metastasis. No close correlation was found between PH, GH and SH on one hand and histological types of the primary gastric carcinomas on the other. These results suggest that cell mediated and/or humoral immune response may play an important role in host resistance against gastric carcinoma.", "contents": "Reactive hyperplasia of paracortex and germinal centers of the regional lymph nodes in gastric carcinoma cases as a favorable prognostic indicator. The prognosis of 141 patients subjected to curative resection for gastric carcinoma was analyzed with special reference to the paracortex, germinal centers and sinus histiocytes in the regional lymph nodes. Five years survival rates of the patients with paracortical and/or germinal center hyperplasia (PH and/or GH) was definitely superior to those without such hyperplasia. This difference was most pronounced in those with moderately advanced stage. The incidence of lymph node metastasis was lower in the patients with PH or GH. The presence of sinus histiocytosis (SH) seemed to be correlated neither with a longer survival nor with lower incidence of lymph node metastasis. No close correlation was found between PH, GH and SH on one hand and histological types of the primary gastric carcinomas on the other. These results suggest that cell mediated and/or humoral immune response may play an important role in host resistance against gastric carcinoma.", "PMID": 978857}
{"id": "pubmed23n0032_11936", "title": "A seven year review of invasive carcinoma of the cervix treated at the Johns Hopkins Hospital.", "content": "172 cases of invasive carcinoma of the cervix treated at the Johns Hopkins Hospital are reviewed. Failure rates are examined by stage, demonstrating a high percentage of local recurrences in patients with late stage disease treated by a traditional regime of radiotherapy. Reasons for these failures are explored and a proposal for a more individualized approach to therapy is made.", "contents": "A seven year review of invasive carcinoma of the cervix treated at the Johns Hopkins Hospital. 172 cases of invasive carcinoma of the cervix treated at the Johns Hopkins Hospital are reviewed. Failure rates are examined by stage, demonstrating a high percentage of local recurrences in patients with late stage disease treated by a traditional regime of radiotherapy. Reasons for these failures are explored and a proposal for a more individualized approach to therapy is made.", "PMID": 978858}
{"id": "pubmed23n0032_11937", "title": "Phenacetin-induced sulfhemoglobinemia: report of a case and review of the literature.", "content": "Phenacetin-induced sulfhemoglobinemia was diagnosed in a middle-aged woman with a 6-month history of cyanosis and a 10-year history of ingestion of analgesics. The laboratory and clincial diagnosis of sulfhemoglobinemia and methemoglobinemia are discussed. The reviewed medical literature suggests that the cyanosis of chronic phenacetin or acetanilid use is generally due to sulfhemoglobinemia rather than methemoglobinemia.", "contents": "Phenacetin-induced sulfhemoglobinemia: report of a case and review of the literature. Phenacetin-induced sulfhemoglobinemia was diagnosed in a middle-aged woman with a 6-month history of cyanosis and a 10-year history of ingestion of analgesics. The laboratory and clincial diagnosis of sulfhemoglobinemia and methemoglobinemia are discussed. The reviewed medical literature suggests that the cyanosis of chronic phenacetin or acetanilid use is generally due to sulfhemoglobinemia rather than methemoglobinemia.", "PMID": 978859}
{"id": "pubmed23n0032_11938", "title": "[Immunological reactivity in postinfarct cardiosclerosis].", "content": "In 77 patients with postinfarction cardiosclerosis the blood serum immunoglobulin level was studied along with some indices of the lipid and protein metabolism. An increased blood level of immunoglobulins A, M and G was observed in postinfarction cardiosclerosis cases, those with hyper-beta-lipoproteinemia, hypercholesterolemia and C-reactive protein having a significantly higher level of immunoglobulin G and than the rest of the patients. In those having an increased content of immunoglobulin G in the blood serum hypo-beta-globulinemia was observed, as well as a considerable increase of the gamma-globulin fraction as compared to those having a normal or decreased level of immunoglobulin G. Proceeding from these data the authors suggest the presence of autoimmune shifts in the patients with postinfarction cardiosclerosis that are closely connected with lipid and protein metabolism disorders.", "contents": "[Immunological reactivity in postinfarct cardiosclerosis]. In 77 patients with postinfarction cardiosclerosis the blood serum immunoglobulin level was studied along with some indices of the lipid and protein metabolism. An increased blood level of immunoglobulins A, M and G was observed in postinfarction cardiosclerosis cases, those with hyper-beta-lipoproteinemia, hypercholesterolemia and C-reactive protein having a significantly higher level of immunoglobulin G and than the rest of the patients. In those having an increased content of immunoglobulin G in the blood serum hypo-beta-globulinemia was observed, as well as a considerable increase of the gamma-globulin fraction as compared to those having a normal or decreased level of immunoglobulin G. Proceeding from these data the authors suggest the presence of autoimmune shifts in the patients with postinfarction cardiosclerosis that are closely connected with lipid and protein metabolism disorders.", "PMID": 978865}
{"id": "pubmed23n0032_11939", "title": "[Intraventricular blocks in myocardial infarct].", "content": "The examination was carried out in 787 patients with macrofocal myocardial infarction. The most frequently encountered variant of intraventricular block in males was the right bundle branch block, in females--the left bundle branch block. The rarest variant of intraventricular conductivity disorders in myocardial infarction was the left-posterior hemiblock. The prognostically severest variant of bilateral block consists in a combination of the right bundle branch block with the left-posterior hemiblock. The leading causes of death among the patients with myocardial infarction and intraventricular blocks were acute (cardiogenic shock, pulmonary oedema) and chronic cardiac insufficiency. In patients with bilateral blocks the frequent causes of death were, along with cardiac insufficiency, also arrhythmias (ventricular fibrillation, asystole).", "contents": "[Intraventricular blocks in myocardial infarct]. The examination was carried out in 787 patients with macrofocal myocardial infarction. The most frequently encountered variant of intraventricular block in males was the right bundle branch block, in females--the left bundle branch block. The rarest variant of intraventricular conductivity disorders in myocardial infarction was the left-posterior hemiblock. The prognostically severest variant of bilateral block consists in a combination of the right bundle branch block with the left-posterior hemiblock. The leading causes of death among the patients with myocardial infarction and intraventricular blocks were acute (cardiogenic shock, pulmonary oedema) and chronic cardiac insufficiency. In patients with bilateral blocks the frequent causes of death were, along with cardiac insufficiency, also arrhythmias (ventricular fibrillation, asystole).", "PMID": 978866}
{"id": "pubmed23n0032_11940", "title": "[Diagnosis of diffuse myocardial changes in ischemic heart disease using orthogonal ECG leads].", "content": "In 100 patients with chronic ischaemic heart disease and diffuse changes in the myocardium the data of 12 common ECG leads indicated that the corrected orthogonal leads according to Mac Fee--Parungao and Frank reflect these changes in all cases. The severity of the diffuse changes reflected in the deviations of the ST segment and the T-wave in the orthogonal leads corresponds to that reflected in the 12 common leads. A mathematical analysis of the waves of the QRS complex in the X, Y and Z leads indicates a growth of the left ventricular potentials with the development of diffuse myocardial changes. The mentioned systems of orthogonal leads can be recommended for wide employment for the diagnosis of diffuse changes in the myocardium in ischaemic heart disease.", "contents": "[Diagnosis of diffuse myocardial changes in ischemic heart disease using orthogonal ECG leads]. In 100 patients with chronic ischaemic heart disease and diffuse changes in the myocardium the data of 12 common ECG leads indicated that the corrected orthogonal leads according to Mac Fee--Parungao and Frank reflect these changes in all cases. The severity of the diffuse changes reflected in the deviations of the ST segment and the T-wave in the orthogonal leads corresponds to that reflected in the 12 common leads. A mathematical analysis of the waves of the QRS complex in the X, Y and Z leads indicates a growth of the left ventricular potentials with the development of diffuse myocardial changes. The mentioned systems of orthogonal leads can be recommended for wide employment for the diagnosis of diffuse changes in the myocardium in ischaemic heart disease.", "PMID": 978867}
{"id": "pubmed23n0032_11941", "title": "[Course and outcome of ischemic heart disease in patients with angiographically confirmed coronary arteriosclerosis].", "content": "The clinical course and prognosis were studied in 116 patients with clinical signs of ischaemic heart disease when they were hospitalized in an institution in which selective coronary angiography was practiced along with the routine clinical examinations. Control examinations were conducted 2 years later. The dynamics of the clinical manifestations and the results of the disease were evaluated according to the following categories: improvement, stabilization, progressive course, death. All the examined patients were divided into 2 groups: 74 patients with atherosclerotic coronary heart disease proved at angiography, and 72 patients without angiographic signs of coronary pathology. The course of the disease was found to depend on the presence and severity of the atherosclerotic process in the coronary arteries. Essential hypertension aggravated the course and prognosis of the disease. The distinctness of the clinical manifestations of coronary insufficiency, the ischaemic changes in the ECG repolarization complex, the duration of the disease history, and the age of the patients tended to predispose the outcome of the atherosclerotic lesion of the heart. In the group of patients with clinical signs of ischaemic heart disease, but without angiographic changes in the coronaries the result was more favourable, than in those with coronary atherosclerosis. This group, however, included some patients with prognostically unfavourable myocarcial lesions of non-atherosclerotic genesis.", "contents": "[Course and outcome of ischemic heart disease in patients with angiographically confirmed coronary arteriosclerosis]. The clinical course and prognosis were studied in 116 patients with clinical signs of ischaemic heart disease when they were hospitalized in an institution in which selective coronary angiography was practiced along with the routine clinical examinations. Control examinations were conducted 2 years later. The dynamics of the clinical manifestations and the results of the disease were evaluated according to the following categories: improvement, stabilization, progressive course, death. All the examined patients were divided into 2 groups: 74 patients with atherosclerotic coronary heart disease proved at angiography, and 72 patients without angiographic signs of coronary pathology. The course of the disease was found to depend on the presence and severity of the atherosclerotic process in the coronary arteries. Essential hypertension aggravated the course and prognosis of the disease. The distinctness of the clinical manifestations of coronary insufficiency, the ischaemic changes in the ECG repolarization complex, the duration of the disease history, and the age of the patients tended to predispose the outcome of the atherosclerotic lesion of the heart. In the group of patients with clinical signs of ischaemic heart disease, but without angiographic changes in the coronaries the result was more favourable, than in those with coronary atherosclerosis. This group, however, included some patients with prognostically unfavourable myocarcial lesions of non-atherosclerotic genesis.", "PMID": 978877}
{"id": "pubmed23n0032_11942", "title": "[Interstitial cells of the medulla and the renin-angiotensin system in disorders of water-salt homeostasis].", "content": "Electron microscopic studies of the intersticial cells of the renal medullar layer and the count of lipid granules in them were conducted in two experimental states accompanied by a reduction of the circulating blood volume--72 hours of water-free diet and intravenous administration of Lazyx that possesses a potent natriuretic and diuretic effect. The concentration of renin and angiotensin was determined in the plasma of the same animals, using the radioimmunoassay technique, and the juxtaglomerular apparatus of the kidneys was examined. The study has demonstrated that the reduction of the circulating blood volume is accompanied, on the one hand, by a significant elevation of the plasma concentration of renin and angiotensin, and, on the other hand, by a reduction of the number of lipid granules in the intersticial cells that is interpreted by the authors as an index of an increased synthesis of renal prostaglandins. It has been concluded that a close functional interrelashionship exists between the two hormonal systems of the kidneys that regulate the water-electrolyte homeostasis, i.e. the renin-angiotensin system and the prostaglandins system.", "contents": "[Interstitial cells of the medulla and the renin-angiotensin system in disorders of water-salt homeostasis]. Electron microscopic studies of the intersticial cells of the renal medullar layer and the count of lipid granules in them were conducted in two experimental states accompanied by a reduction of the circulating blood volume--72 hours of water-free diet and intravenous administration of Lazyx that possesses a potent natriuretic and diuretic effect. The concentration of renin and angiotensin was determined in the plasma of the same animals, using the radioimmunoassay technique, and the juxtaglomerular apparatus of the kidneys was examined. The study has demonstrated that the reduction of the circulating blood volume is accompanied, on the one hand, by a significant elevation of the plasma concentration of renin and angiotensin, and, on the other hand, by a reduction of the number of lipid granules in the intersticial cells that is interpreted by the authors as an index of an increased synthesis of renal prostaglandins. It has been concluded that a close functional interrelashionship exists between the two hormonal systems of the kidneys that regulate the water-electrolyte homeostasis, i.e. the renin-angiotensin system and the prostaglandins system.", "PMID": 978878}
{"id": "pubmed23n0032_11943", "title": "[Content of total metabolic sodium in arterial hypertension of varying origin].", "content": "The content of total metabolizing sodium was studied in 74 patients with hypertension of different genesis, using the Na24-isotope dilution technique. Among these patients 31 had essential hypertension, 43--symptomatic hypertension (40--renal, and 3--adrenal). In Stage IB and IIA hypertension, a reduction of the level of total metabolizing sodium and its increased urine excretion were found. At late stages of essential hypertension, like in symptomatic renal hypertension, normal levels of total metabolizing sodium were found, or a slight tendency towards its elevation. In cases of adrenal hypertension (Conn's syndrome, pheochromocytoma) the level of total metabolizing sodium is significantly elevated. No correlation was seen between the levels of total metabolizing sodium, and plasma and erythrocytes sodium. The decrease of total metabolizing sodium at early stages of essential hypertension must be an adaptative reaction of the body, which is proved by the increased urine excretion of sodium.", "contents": "[Content of total metabolic sodium in arterial hypertension of varying origin]. The content of total metabolizing sodium was studied in 74 patients with hypertension of different genesis, using the Na24-isotope dilution technique. Among these patients 31 had essential hypertension, 43--symptomatic hypertension (40--renal, and 3--adrenal). In Stage IB and IIA hypertension, a reduction of the level of total metabolizing sodium and its increased urine excretion were found. At late stages of essential hypertension, like in symptomatic renal hypertension, normal levels of total metabolizing sodium were found, or a slight tendency towards its elevation. In cases of adrenal hypertension (Conn's syndrome, pheochromocytoma) the level of total metabolizing sodium is significantly elevated. No correlation was seen between the levels of total metabolizing sodium, and plasma and erythrocytes sodium. The decrease of total metabolizing sodium at early stages of essential hypertension must be an adaptative reaction of the body, which is proved by the increased urine excretion of sodium.", "PMID": 978879}
{"id": "pubmed23n0032_11944", "title": "[Atherosclerotic (systolic) hypertension].", "content": "Systolic hypertension was studied in 92 patients, aged 60 to 103 years, some of them being followed-up for periods up to 8 years. The examination included that of haemodynamics, functional state of the heart and gas composition of blood (by the dye dilution technique, polycardiography, teleradiography, Sechenov and van Slyke methods, the micro-Astrup technique). The previously reported concept of the author was proved, i.e. the immediate haemodynamic cause of arterial pressure elevation in this pathology consists in an increased cardiac output against the background of an increased total elastic resistance of the arterial system. The elevation of the cardiac output is due not only to the neuro-humoral disorders in the regulation of arterial pressure in old age, but also to the adaptational factors directed to the improvement of oxygen transport and consumption by the tissues when the premeability of the vascular-tissuemembranes is impaired.", "contents": "[Atherosclerotic (systolic) hypertension]. Systolic hypertension was studied in 92 patients, aged 60 to 103 years, some of them being followed-up for periods up to 8 years. The examination included that of haemodynamics, functional state of the heart and gas composition of blood (by the dye dilution technique, polycardiography, teleradiography, Sechenov and van Slyke methods, the micro-Astrup technique). The previously reported concept of the author was proved, i.e. the immediate haemodynamic cause of arterial pressure elevation in this pathology consists in an increased cardiac output against the background of an increased total elastic resistance of the arterial system. The elevation of the cardiac output is due not only to the neuro-humoral disorders in the regulation of arterial pressure in old age, but also to the adaptational factors directed to the improvement of oxygen transport and consumption by the tissues when the premeability of the vascular-tissuemembranes is impaired.", "PMID": 978880}
{"id": "pubmed23n0032_11945", "title": "[Experimental and clinical data on the state of the atria in postinfarct cardiosclerosis].", "content": "In 16 rabbit experiments with macrofocal postinfarction cardiosclerosis and 8 control intact animals delayed electron microscopy of myocardium portions from the left ventricle and atria was undertaken, and in 76 clinical observations of patients who had survived a myocardial infarction 1 to 10 years before the examination the indices of the haemodynamic and electrical activity of the atria were compared with those of the contractile function of the left ventricular myocardium under bicycle exercise tests of a growing intensity (with the data of the left ventricular ultrastructure--in the experimental cases). The changes in the left atrium were found to be of an adaptational nature, the right atrium entering the pathogenetic chain when the compensatory capacities of the left atrium got exhausted in the course of the disease, this being a prognostically alerting sign.", "contents": "[Experimental and clinical data on the state of the atria in postinfarct cardiosclerosis]. In 16 rabbit experiments with macrofocal postinfarction cardiosclerosis and 8 control intact animals delayed electron microscopy of myocardium portions from the left ventricle and atria was undertaken, and in 76 clinical observations of patients who had survived a myocardial infarction 1 to 10 years before the examination the indices of the haemodynamic and electrical activity of the atria were compared with those of the contractile function of the left ventricular myocardium under bicycle exercise tests of a growing intensity (with the data of the left ventricular ultrastructure--in the experimental cases). The changes in the left atrium were found to be of an adaptational nature, the right atrium entering the pathogenetic chain when the compensatory capacities of the left atrium got exhausted in the course of the disease, this being a prognostically alerting sign.", "PMID": 978881}
{"id": "pubmed23n0032_11946", "title": "[Can myopia be influenced by the wearing of contact lenses?].", "content": "During 15 years the author has had the occasion to examine 11,454 spectacle wearing patients and 6,794 contact lens-wearing patients. In the age-group 15 to 25 years the contact lens-wearing patients manifested in 60% of the cases stationary myopia, whereas the spectacle-wearing patients in the same period manifested an increase of myopia in 75% of the cases. For a comparison in each patients visual acuity with and later without contact lens was determined, refraction performed and a slit lamp examination realized with and without contact lens. Determination of axial length of the eyes with the aid of ultrasonography could not be performed. The result of this analysis is compared with the results of other authors who have delt with the same problem. The author tries to give an explanation for the distinctly reduced increase of myopia in patients with contact lenses compared with those wearing glasses.", "contents": "[Can myopia be influenced by the wearing of contact lenses?]. During 15 years the author has had the occasion to examine 11,454 spectacle wearing patients and 6,794 contact lens-wearing patients. In the age-group 15 to 25 years the contact lens-wearing patients manifested in 60% of the cases stationary myopia, whereas the spectacle-wearing patients in the same period manifested an increase of myopia in 75% of the cases. For a comparison in each patients visual acuity with and later without contact lens was determined, refraction performed and a slit lamp examination realized with and without contact lens. Determination of axial length of the eyes with the aid of ultrasonography could not be performed. The result of this analysis is compared with the results of other authors who have delt with the same problem. The author tries to give an explanation for the distinctly reduced increase of myopia in patients with contact lenses compared with those wearing glasses.", "PMID": 979024}
{"id": "pubmed23n0032_11947", "title": "[A new iris double-forceps in the cryoextraction of cataract (author's transl)].", "content": "In the cryoextraction of a cataract associated with severe hypotension and narrow pupil, a new smooth iris double-forceps has been found useful for the grasping of the iris and the dilatation of the pupil. Thus, it will be possible to obtain a round pupil even in these rare cases.", "contents": "[A new iris double-forceps in the cryoextraction of cataract (author's transl)]. In the cryoextraction of a cataract associated with severe hypotension and narrow pupil, a new smooth iris double-forceps has been found useful for the grasping of the iris and the dilatation of the pupil. Thus, it will be possible to obtain a round pupil even in these rare cases.", "PMID": 979025}
{"id": "pubmed23n0032_11948", "title": "[Vitreoretinal dystrophy with vitreous haemorrhage (author's transl)].", "content": "In two generations of a big family two men in each generation were struck with a vitreoretinal disease, which led to vitreous haemorrhage and were first was interpreted as Periphlebitis retinae (Eale's disease). Preretinal, perforated membranes containing vessels and a severe macular degeneration pointed to a vitreoretinal dystrophy. The uniform alteration of the ERG with reduction of the b-waves were striking in all four patients. Judging from the X-chromosomal pattern of transmission, from the juvenile vitreous haemorrhages and from the macular dysplasia with radial plication we seem to be dealing with a sexlinked juvenile retinoschisis, although at first microscopic examination the vitreous veils did not make us think of a retinoschisis.", "contents": "[Vitreoretinal dystrophy with vitreous haemorrhage (author's transl)]. In two generations of a big family two men in each generation were struck with a vitreoretinal disease, which led to vitreous haemorrhage and were first was interpreted as Periphlebitis retinae (Eale's disease). Preretinal, perforated membranes containing vessels and a severe macular degeneration pointed to a vitreoretinal dystrophy. The uniform alteration of the ERG with reduction of the b-waves were striking in all four patients. Judging from the X-chromosomal pattern of transmission, from the juvenile vitreous haemorrhages and from the macular dysplasia with radial plication we seem to be dealing with a sexlinked juvenile retinoschisis, although at first microscopic examination the vitreous veils did not make us think of a retinoschisis.", "PMID": 979026}
{"id": "pubmed23n0032_11949", "title": "[Gas gangrene panophthalmitis (author's transl)].", "content": "Gas gangrene panophthalmitis is a rare condition of penetrating injury to the globe. The infecting organism is usually Clostridium perfringens. Characteristic symptoms are a brawny swelling of the lids, marked chemosis, coffee-coloured discharge, hypopyon, ring abscess of the cornea, formation of gas bubbles in the anterior chamber, rise of intraocular tension and early amaurosis. Treatment consists in the evisceration or enucleation of the globe, rarely in the exenteration of the orbit. Antibiotics along (Penicillin, Tetracyclines) are insufficient. Administration of antiserum is almost completely abandoned, it is probably more dangerous than helpful. The use of hyperbaric oxygen is not indicated in cases of gas gangrene panophthalmitis. Extraocular extension of the infection and its danger for the individual is prevented by well-times surgical procedure.", "contents": "[Gas gangrene panophthalmitis (author's transl)]. Gas gangrene panophthalmitis is a rare condition of penetrating injury to the globe. The infecting organism is usually Clostridium perfringens. Characteristic symptoms are a brawny swelling of the lids, marked chemosis, coffee-coloured discharge, hypopyon, ring abscess of the cornea, formation of gas bubbles in the anterior chamber, rise of intraocular tension and early amaurosis. Treatment consists in the evisceration or enucleation of the globe, rarely in the exenteration of the orbit. Antibiotics along (Penicillin, Tetracyclines) are insufficient. Administration of antiserum is almost completely abandoned, it is probably more dangerous than helpful. The use of hyperbaric oxygen is not indicated in cases of gas gangrene panophthalmitis. Extraocular extension of the infection and its danger for the individual is prevented by well-times surgical procedure.", "PMID": 979027}
{"id": "pubmed23n0032_11950", "title": "[Statistical importance of tuberculosis and toxoplasmosis of the eye in recent years (1971-1974, Eye-Clinic, Davos) (author's transl)].", "content": "Tbc has not yet been eradicated in Western Europe. Among 311 human/bovin-tuberculin tested cases out of 500 patients with uveitis (1971-1974), 37-50% (average age 40-45 years) reacted highly positive. Chorioretinitis disseminata and periphlebitis retinae (Eales' disease): 90% positive. The criteria for a long term tuberculostatic treatment are exposed. 20% of all cases of uveitis and 90% of Jensen Retinochorioditis have been interpreted as caused by toxoplasmosis. 75% are relapses. The gigantic toxoplasmotic lesions of the eyeground is stressed. As a rule low Sabin Feldmann titers are found. Quantitative serology is valuable. In the closing discussion the immunologically well known adjuvans-effect of Koch's bacillus-extract is supposed to be of importance in highly tuberculin positive uveitis.", "contents": "[Statistical importance of tuberculosis and toxoplasmosis of the eye in recent years (1971-1974, Eye-Clinic, Davos) (author's transl)]. Tbc has not yet been eradicated in Western Europe. Among 311 human/bovin-tuberculin tested cases out of 500 patients with uveitis (1971-1974), 37-50% (average age 40-45 years) reacted highly positive. Chorioretinitis disseminata and periphlebitis retinae (Eales' disease): 90% positive. The criteria for a long term tuberculostatic treatment are exposed. 20% of all cases of uveitis and 90% of Jensen Retinochorioditis have been interpreted as caused by toxoplasmosis. 75% are relapses. The gigantic toxoplasmotic lesions of the eyeground is stressed. As a rule low Sabin Feldmann titers are found. Quantitative serology is valuable. In the closing discussion the immunologically well known adjuvans-effect of Koch's bacillus-extract is supposed to be of importance in highly tuberculin positive uveitis.", "PMID": 979028}
{"id": "pubmed23n0032_11951", "title": "[Eye symptoms in the subacute necrotizing encephalomyelopathy (author's transl)].", "content": "A 30 years old man developed Parinaud's syndrome without other neurological symptoms. The X-ray-examination was normal. The autopsy revealed a subacute necrotizing encephalomyelopathy. The symptoms, pathology and possible aetiology are discussed.", "contents": "[Eye symptoms in the subacute necrotizing encephalomyelopathy (author's transl)]. A 30 years old man developed Parinaud's syndrome without other neurological symptoms. The X-ray-examination was normal. The autopsy revealed a subacute necrotizing encephalomyelopathy. The symptoms, pathology and possible aetiology are discussed.", "PMID": 979029}
{"id": "pubmed23n0032_11952", "title": "[Keratoconus and contact lenses (author's transl)].", "content": "This work is a survey of 82 cases of keratoconus which have been followed up for 1 to 12 years. Among them only 66 were fitted with contact lenses. The contra-indications for them are: 1. a better visual acuity with spectacles than with contact lenses, 2. advanced cases (4th degree of Amsler) whose fitting is impossible, 3. unilateral keratoconus, 4. associated diseases such as trachomatous pannus, allergic kerato-conjunctivitis. Hard corneal lenses are now in use in most of the cases. Scleral lenses are much less used than they were 10 years ago, owing probably to the great improvement of the corneal lenses during this time. These hard corneal lenses have a short Ro (4 to 7 mm), an overall diameter between 8 and 11 mm, and an optic diameter of 5 mm. They are fitted under fluorescein control. The mobility must be good too. One case was fitted with soft lenses. The visual acuity is good and so is the tolerance: 80% of the patients wear their lenses 10 hours a day or more. Contact lenses do not affect the progression of keratoconus thus finally a keratoplasty must be performed in many cases. After the operation a contact lens is very often necessary, but its daily wearing time must be divided by two, to avoid corneal neo-vascularisation. Soft corneal lenses may be used in some cases of keratoconus. They are indicated when the hard lenses are no longer tolerated and before a keratoplasty. The base curves of these soft lenses are not related to the radii of the conic cornea. In most of the cases they are between 7.50 and 8.60 mm. The diameter is large: 14 or 15 mm. The lenses must not move too much: 1 mm up or down when the patient blinks. The edge of the lens must not depress the bulbar conjunctiva and there must be no air bubble under the lens. In many cases a cylindrical spectacle lens is necessary to obtain a good visual acuity. Some authors prefer to fit a hard corneal lens over the soft one: this is the \"piggy back\" method. Sometimes keratoconus has appeared in patients already fitted during several years to correct a myopic astigmatism. It is not clear whether these keratoconus have been produced or not be the contact lenses.", "contents": "[Keratoconus and contact lenses (author's transl)]. This work is a survey of 82 cases of keratoconus which have been followed up for 1 to 12 years. Among them only 66 were fitted with contact lenses. The contra-indications for them are: 1. a better visual acuity with spectacles than with contact lenses, 2. advanced cases (4th degree of Amsler) whose fitting is impossible, 3. unilateral keratoconus, 4. associated diseases such as trachomatous pannus, allergic kerato-conjunctivitis. Hard corneal lenses are now in use in most of the cases. Scleral lenses are much less used than they were 10 years ago, owing probably to the great improvement of the corneal lenses during this time. These hard corneal lenses have a short Ro (4 to 7 mm), an overall diameter between 8 and 11 mm, and an optic diameter of 5 mm. They are fitted under fluorescein control. The mobility must be good too. One case was fitted with soft lenses. The visual acuity is good and so is the tolerance: 80% of the patients wear their lenses 10 hours a day or more. Contact lenses do not affect the progression of keratoconus thus finally a keratoplasty must be performed in many cases. After the operation a contact lens is very often necessary, but its daily wearing time must be divided by two, to avoid corneal neo-vascularisation. Soft corneal lenses may be used in some cases of keratoconus. They are indicated when the hard lenses are no longer tolerated and before a keratoplasty. The base curves of these soft lenses are not related to the radii of the conic cornea. In most of the cases they are between 7.50 and 8.60 mm. The diameter is large: 14 or 15 mm. The lenses must not move too much: 1 mm up or down when the patient blinks. The edge of the lens must not depress the bulbar conjunctiva and there must be no air bubble under the lens. In many cases a cylindrical spectacle lens is necessary to obtain a good visual acuity. Some authors prefer to fit a hard corneal lens over the soft one: this is the \"piggy back\" method. Sometimes keratoconus has appeared in patients already fitted during several years to correct a myopic astigmatism. It is not clear whether these keratoconus have been produced or not be the contact lenses.", "PMID": 979030}
{"id": "pubmed23n0032_11953", "title": "[The correction of anisomyopia with contact lenses (author's transl)].", "content": "Currently it is believed that correction of anisomyopia with contact lenses is contraindicated because most anisomyopias are axial. By determination of dioptric aniseiconia it has been shown that this is smaller than generally believed also with the correction by contact lenses. In childhood it is advisable to correct an anisomyopia fully as early as possible because the distortion of room perception by spectacles seems to play a much bigger role in higher anisomyopia than the functional aniseiconia. The results are shown in tables.", "contents": "[The correction of anisomyopia with contact lenses (author's transl)]. Currently it is believed that correction of anisomyopia with contact lenses is contraindicated because most anisomyopias are axial. By determination of dioptric aniseiconia it has been shown that this is smaller than generally believed also with the correction by contact lenses. In childhood it is advisable to correct an anisomyopia fully as early as possible because the distortion of room perception by spectacles seems to play a much bigger role in higher anisomyopia than the functional aniseiconia. The results are shown in tables.", "PMID": 979032}
{"id": "pubmed23n0032_11954", "title": "[Special indications for the use of soft contact lenses as a drug-release-system (author's transl)].", "content": "Research has been performed, both experimentally and clinically, to establish the value of the association of soft contact lenses and some types of eye drops. The use of soft contact lenses with eye drops may be useful in some special cases: a) more prolonged and more sustained effect compared with the usual way of administration of eye drops (especially antiglaucomatous substances, antimetabolites, mydriatics); b) possibility of reducing the concentration to avoid local discomfort or systemic side-effects, without loss of their effectiveness on the eye conditions to be treated. The combined use of soft lenses (12.5-15 mm in diameter) with eye drops may be obtained either by presoaking the lens in the liquid or by regular instillation of eye drops after insertion of the lens; the two techniques may of course be associated. In the present research the advantages of utilizing hydrophylic lenses with osmotically active substances, to obtain a better and more protracted dehydration of the cornea, were first examined, in vitro and in vivo. The following substances were tested: 10% propylenglycol, 10% glycerol, 10% glucose and 5% natrium chloride. The clearing effect of the different types of treatment was evaluated in 45 patients with edematous bullous keratopathy with an instrument which measured the infrared light emitted by an optic fiber and reflected by the cornea. The effects were more marked for the epithelial than for the stromal oedema. Another group of investigations was performed with two polypeptides with high molecular weight: Eledoisin, extracted from a mediterranean octopus, Eledone moschata, and Physalaemin, extracted from the skin of a south american batrachian, Physalaemus fuscomaculatus, both of these stimulate the lacrimal secretion and were previously successfully employed topically by the authors against keratoconjunctivitis sicca. The increase of the amount of fluid was however short-lived. Eledoisin at a concentration of 200 mug/ml, was examined in its effects both in vitro and in vivo, whereas physalaemin, at a concentration of 20 mug/ml, only in vitro, owing to the present shortage of the product. The clinical tests in 23 eyes of 14 patients with keratoconjunctivitis sicca proved satisfactory, since the lacrymal stimulating effect is not only greater, but lasts three times longer by combining the instillation of eledoisin with a presoaked soft lens. Some antiglaucomatous products (propranolol, clonidine, prostigmine) were, finally, used in association with a soft lens to reduce the concentration of the eye drops for a better tolerance locally (propranolol: a beta-adrenergic blocking agent) or generally (clonidine: alpha-adrenergic agent), also with the advantage of protracted release. With propranolol the concentration could be reduced to 0.01-0,10% (instead of 0.125 to 0.25%) and to 1.5% (instead of 3%) with prostigmine, when lenses were presoaked or instillations took place at regular time intervals, after insertion of the lenses.", "contents": "[Special indications for the use of soft contact lenses as a drug-release-system (author's transl)]. Research has been performed, both experimentally and clinically, to establish the value of the association of soft contact lenses and some types of eye drops. The use of soft contact lenses with eye drops may be useful in some special cases: a) more prolonged and more sustained effect compared with the usual way of administration of eye drops (especially antiglaucomatous substances, antimetabolites, mydriatics); b) possibility of reducing the concentration to avoid local discomfort or systemic side-effects, without loss of their effectiveness on the eye conditions to be treated. The combined use of soft lenses (12.5-15 mm in diameter) with eye drops may be obtained either by presoaking the lens in the liquid or by regular instillation of eye drops after insertion of the lens; the two techniques may of course be associated. In the present research the advantages of utilizing hydrophylic lenses with osmotically active substances, to obtain a better and more protracted dehydration of the cornea, were first examined, in vitro and in vivo. The following substances were tested: 10% propylenglycol, 10% glycerol, 10% glucose and 5% natrium chloride. The clearing effect of the different types of treatment was evaluated in 45 patients with edematous bullous keratopathy with an instrument which measured the infrared light emitted by an optic fiber and reflected by the cornea. The effects were more marked for the epithelial than for the stromal oedema. Another group of investigations was performed with two polypeptides with high molecular weight: Eledoisin, extracted from a mediterranean octopus, Eledone moschata, and Physalaemin, extracted from the skin of a south american batrachian, Physalaemus fuscomaculatus, both of these stimulate the lacrimal secretion and were previously successfully employed topically by the authors against keratoconjunctivitis sicca. The increase of the amount of fluid was however short-lived. Eledoisin at a concentration of 200 mug/ml, was examined in its effects both in vitro and in vivo, whereas physalaemin, at a concentration of 20 mug/ml, only in vitro, owing to the present shortage of the product. The clinical tests in 23 eyes of 14 patients with keratoconjunctivitis sicca proved satisfactory, since the lacrymal stimulating effect is not only greater, but lasts three times longer by combining the instillation of eledoisin with a presoaked soft lens. Some antiglaucomatous products (propranolol, clonidine, prostigmine) were, finally, used in association with a soft lens to reduce the concentration of the eye drops for a better tolerance locally (propranolol: a beta-adrenergic blocking agent) or generally (clonidine: alpha-adrenergic agent), also with the advantage of protracted release. With propranolol the concentration could be reduced to 0.01-0,10% (instead of 0.125 to 0.25%) and to 1.5% (instead of 3%) with prostigmine, when lenses were presoaked or instillations took place at regular time intervals, after insertion of the lenses.", "PMID": 979031}
{"id": "pubmed23n0032_11955", "title": "[Introduction to problems of plastic surgery of the eyelids (author's transl)].", "content": "The qualities of the plastic surgeon and the basic principles of plastic surgery are briefly described, particularly in relation to the reconstruction of wounded eyelids.", "contents": "[Introduction to problems of plastic surgery of the eyelids (author's transl)]. The qualities of the plastic surgeon and the basic principles of plastic surgery are briefly described, particularly in relation to the reconstruction of wounded eyelids.", "PMID": 979033}
{"id": "pubmed23n0032_11956", "title": "[Differential diagnosis of ptosis (author's transl)].", "content": "An accurate differential diagnosis of ptosis results if one refers the functional disorders of the levator to the different levels of the motor unit: Muscle, synapsis, peripheral neuron and nucleus. Therefore ptosis can be classified into myogenic, synaptogenic and neurogenic forms. This classification is also based on electromyographic findings from the levator muscle. Myogenic forms of the ptosis are: Senile ptosis, chronic progressive ocular muscle dystrophy, acute exophthalmic myositis and affections of the levator together with general systemic myopathies. Synaptogenic forms of ptosis are: Botulism and myasthenia gravis. Neurogenic forms of ptosis are: Cortical ptosis, ptosis from lesions of the oculomotor nucleus (congenital ptosis, Moebius-syndrome), peripheral oculomotor palsy, ptosis in aberrant third nerve regeneration, sympathetic ptosis (Horner-syndrome), ptosis in cases of paradoxic innervation such as the Duane retraction-syndrome and the Marcus-Gunn-Jaw-Winking-syndrome (ptosis linked with jaw movement). According to the classification in myogenic, synaptogenic and peripheral-neurogenic forms of ptosis, the therapeutic possibilities (medical, surgical etc.) are discussed.", "contents": "[Differential diagnosis of ptosis (author's transl)]. An accurate differential diagnosis of ptosis results if one refers the functional disorders of the levator to the different levels of the motor unit: Muscle, synapsis, peripheral neuron and nucleus. Therefore ptosis can be classified into myogenic, synaptogenic and neurogenic forms. This classification is also based on electromyographic findings from the levator muscle. Myogenic forms of the ptosis are: Senile ptosis, chronic progressive ocular muscle dystrophy, acute exophthalmic myositis and affections of the levator together with general systemic myopathies. Synaptogenic forms of ptosis are: Botulism and myasthenia gravis. Neurogenic forms of ptosis are: Cortical ptosis, ptosis from lesions of the oculomotor nucleus (congenital ptosis, Moebius-syndrome), peripheral oculomotor palsy, ptosis in aberrant third nerve regeneration, sympathetic ptosis (Horner-syndrome), ptosis in cases of paradoxic innervation such as the Duane retraction-syndrome and the Marcus-Gunn-Jaw-Winking-syndrome (ptosis linked with jaw movement). According to the classification in myogenic, synaptogenic and peripheral-neurogenic forms of ptosis, the therapeutic possibilities (medical, surgical etc.) are discussed.", "PMID": 979034}
{"id": "pubmed23n0032_11957", "title": "[Results of the modified dacryocystorhinostomie described by Werb (author's transl)].", "content": "42 cases operated by the modified Werb's dacryocystorhinostomy with polyethylene tubing are reported. The postoperative follow-up was between 1 and 4 1/2 years. The success rate was 75%. The tube should remain at least 3-4 months. A longer time does not give significantly better results. On 2 unsuccessfully operated persons strong fibrous scar tissue was found during the reoperation at the site of the anastomosis of the tear sack with the nasal mucous membrane. This was probably induced by remaining bone fragments. This simple operative approach can be recommended as a first surgical intervention on patients with a presaccal tear duct stenosis.", "contents": "[Results of the modified dacryocystorhinostomie described by Werb (author's transl)]. 42 cases operated by the modified Werb's dacryocystorhinostomy with polyethylene tubing are reported. The postoperative follow-up was between 1 and 4 1/2 years. The success rate was 75%. The tube should remain at least 3-4 months. A longer time does not give significantly better results. On 2 unsuccessfully operated persons strong fibrous scar tissue was found during the reoperation at the site of the anastomosis of the tear sack with the nasal mucous membrane. This was probably induced by remaining bone fragments. This simple operative approach can be recommended as a first surgical intervention on patients with a presaccal tear duct stenosis.", "PMID": 979035}
{"id": "pubmed23n0032_11958", "title": "[When should congenital lacrimal fistulae be operated? (AUTHOR'S TRANSL)].", "content": "In most cases of congenital lacrimal fistulae occuring in the ophthalmic literature the condition is diagnosed only during later life. Then, however, the congenital nature is doubtful. This paper describes a new-born with bilateral congenital lacrimal fistulae. Furthermore, the underfed patient has heart and genital malformations. The indication for surgical management of congenital lacrimal fistulae is discussed.", "contents": "[When should congenital lacrimal fistulae be operated? (AUTHOR'S TRANSL)]. In most cases of congenital lacrimal fistulae occuring in the ophthalmic literature the condition is diagnosed only during later life. Then, however, the congenital nature is doubtful. This paper describes a new-born with bilateral congenital lacrimal fistulae. Furthermore, the underfed patient has heart and genital malformations. The indication for surgical management of congenital lacrimal fistulae is discussed.", "PMID": 979036}
{"id": "pubmed23n0032_11959", "title": "[Critical flicker frequency (CFF) in the central and peripheral visual field (author's transl)].", "content": "Two methods with their instruments are presented. First the measurement of C.F.F. in modified Goldmann perimeter. Secondly the register of the trimodal curve. The term \"flicker acuity\" is proposed with its mathematical translation. The second method concerns an original papillophotometer which can perhaps explore the color vision mechanism.", "contents": "[Critical flicker frequency (CFF) in the central and peripheral visual field (author's transl)]. Two methods with their instruments are presented. First the measurement of C.F.F. in modified Goldmann perimeter. Secondly the register of the trimodal curve. The term \"flicker acuity\" is proposed with its mathematical translation. The second method concerns an original papillophotometer which can perhaps explore the color vision mechanism.", "PMID": 979037}
{"id": "pubmed23n0032_11960", "title": "[The visually evoked cortical potentials (VECPs) in optic neuritis (author's transl)].", "content": "The visually evoked cortical potentials (VECPs) were examined in 22 patients with optic neuritis. 1. In the acute stage, amplitude and latency time of the VECPs are impaired not only on the affected eye, but often also of the fellow eye. 2. A bilateral disturbance of the VECPs often persists in the recovery period, although psychophysical functions, including color vision remain normal. 3. It follows that in cases of unilateral optic neuritis, demyelinations without clinical evidence may occur in the optic nerve of the fellow eye.", "contents": "[The visually evoked cortical potentials (VECPs) in optic neuritis (author's transl)]. The visually evoked cortical potentials (VECPs) were examined in 22 patients with optic neuritis. 1. In the acute stage, amplitude and latency time of the VECPs are impaired not only on the affected eye, but often also of the fellow eye. 2. A bilateral disturbance of the VECPs often persists in the recovery period, although psychophysical functions, including color vision remain normal. 3. It follows that in cases of unilateral optic neuritis, demyelinations without clinical evidence may occur in the optic nerve of the fellow eye.", "PMID": 979038}
{"id": "pubmed23n0032_11961", "title": "[A new argon-laser-approach for the management of aphakic pupillary block (author's transl)].", "content": "9 cases of aphakic pupillary block with high intraocular pressure were managed successfully by means of a new argon-laser-technique. This new approach consisted in the application at the iris border, 1.5 mm from the pupillary border, of a mobile argon-laser beam of variable intensity, ranging from 250 to 500 mw, of constant time (0.5 sec) and of fixed spot size (200 mu). In some instances the beam was moved parallel and in other cases vertically to the pupillary border in the direction towards the ciliary portion of the iris. This technique ensures maximum retraction of the pupillary border, corresponding to the line of running application, coupled with the least possible damage to the iris and a minimum of applications. Simultaneously with the retraction of the pupillary border the posterior synechiae of the iris are detached and the out-flow of the aqueous trapped in the posterior chamber is released.", "contents": "[A new argon-laser-approach for the management of aphakic pupillary block (author's transl)]. 9 cases of aphakic pupillary block with high intraocular pressure were managed successfully by means of a new argon-laser-technique. This new approach consisted in the application at the iris border, 1.5 mm from the pupillary border, of a mobile argon-laser beam of variable intensity, ranging from 250 to 500 mw, of constant time (0.5 sec) and of fixed spot size (200 mu). In some instances the beam was moved parallel and in other cases vertically to the pupillary border in the direction towards the ciliary portion of the iris. This technique ensures maximum retraction of the pupillary border, corresponding to the line of running application, coupled with the least possible damage to the iris and a minimum of applications. Simultaneously with the retraction of the pupillary border the posterior synechiae of the iris are detached and the out-flow of the aqueous trapped in the posterior chamber is released.", "PMID": 979039}
{"id": "pubmed23n0032_11962", "title": "[The frequency of various forms of senile cataract (author's transl)].", "content": "On classifying 1000 senile cataracts it was found that in pure cataract forms - starting from the cortex - the typical grey senile cataract (cataracta supranuclearis) mostly occurs with a clear nucleus. The dense permeability cataract (cataracta subcapsularis) most frequently shows a dense grey clouding of the nucleus and in addition a still smaller increase in frequency with a clear nucleus. The cortex was also frequently clear when the nucleus of the lens was a dense cloudy grey. Conversley, starting from pure nuclear cataract forms, the dense grey clouded lens nucleus most frequently accompanies a permeability cataract (cataracta subcapsularis) and - with a second peak - with a clear cortex. The brown (black) nucleus (cataracta brunescens [nigral]) quite predominantly shows a clear cortex. If all the cataracts, including the mixed forms between the various cortical and nuclear cataracts are so classified that the percentage participation of the individual forms of cataract in the entire collective (including the mixed forms) is summarised, then the distribution is: Typical grey senile cataract (cataracta supranuclearis) in 23%. Permeability cataract (cataracta subcapsularis) in 36%. Light brown-grey nuclear cataract (mainly nuclear sclerosis) in 13%, grey nuclear cataract in 37%, brown nuclear cataract in 5%, mature cataract in 4%, hypermature cataract in 1%, intumescent cataract in 11%.", "contents": "[The frequency of various forms of senile cataract (author's transl)]. On classifying 1000 senile cataracts it was found that in pure cataract forms - starting from the cortex - the typical grey senile cataract (cataracta supranuclearis) mostly occurs with a clear nucleus. The dense permeability cataract (cataracta subcapsularis) most frequently shows a dense grey clouding of the nucleus and in addition a still smaller increase in frequency with a clear nucleus. The cortex was also frequently clear when the nucleus of the lens was a dense cloudy grey. Conversley, starting from pure nuclear cataract forms, the dense grey clouded lens nucleus most frequently accompanies a permeability cataract (cataracta subcapsularis) and - with a second peak - with a clear cortex. The brown (black) nucleus (cataracta brunescens [nigral]) quite predominantly shows a clear cortex. If all the cataracts, including the mixed forms between the various cortical and nuclear cataracts are so classified that the percentage participation of the individual forms of cataract in the entire collective (including the mixed forms) is summarised, then the distribution is: Typical grey senile cataract (cataracta supranuclearis) in 23%. Permeability cataract (cataracta subcapsularis) in 36%. Light brown-grey nuclear cataract (mainly nuclear sclerosis) in 13%, grey nuclear cataract in 37%, brown nuclear cataract in 5%, mature cataract in 4%, hypermature cataract in 1%, intumescent cataract in 11%.", "PMID": 979040}
{"id": "pubmed23n0032_11963", "title": "[Aging of the lens and cataract (author's transl)].", "content": "Lens opacities in old patients are usually classified as \"senile cataracts\". Since there are very distinct morphologic types of opacities it is evident that we have to deal with different types of triggers in discussing the cataractogenesis. A great variety of risk factors might act as triggers for the development of opacities in aged people, and there is no doubt that among them age changes of lens metabolism are the most important. These age induced changes of lens metabolism do not only influence the energy level of lens metabolism (energy concept of lens transparency), the trigger may as well be located within the protein synthesizing system or the processes responsible for maintaining normal protein conformation, further, an exogenous intoxication may also occur. Lack of normal human lenses of all ages suited for metabolic research and an undefined classification for instance, coloration of the cataractous lens) makes biochemical research with respect to the pathogenesis of senile cataracts almost impossible. Animal models (naphthalene cataract, tryptophane-deficiency, X-irradiation) suited for the purpose may well support the basic research of this subject, even if we have to assume certain species differences of lens metabolism.", "contents": "[Aging of the lens and cataract (author's transl)]. Lens opacities in old patients are usually classified as \"senile cataracts\". Since there are very distinct morphologic types of opacities it is evident that we have to deal with different types of triggers in discussing the cataractogenesis. A great variety of risk factors might act as triggers for the development of opacities in aged people, and there is no doubt that among them age changes of lens metabolism are the most important. These age induced changes of lens metabolism do not only influence the energy level of lens metabolism (energy concept of lens transparency), the trigger may as well be located within the protein synthesizing system or the processes responsible for maintaining normal protein conformation, further, an exogenous intoxication may also occur. Lack of normal human lenses of all ages suited for metabolic research and an undefined classification for instance, coloration of the cataractous lens) makes biochemical research with respect to the pathogenesis of senile cataracts almost impossible. Animal models (naphthalene cataract, tryptophane-deficiency, X-irradiation) suited for the purpose may well support the basic research of this subject, even if we have to assume certain species differences of lens metabolism.", "PMID": 979041}
{"id": "pubmed23n0032_11964", "title": "[Subnormal binocular vision -- principles for the classification of microstrabismus (author's transl)].", "content": "The studies of several hundred case records, most of them carefully observed for years, show that a simple definition of microstrabismus is not sufficient. Contrary to usual opinions about correspondence problems 7% of these cases were even found to be corresponding normally. One third of the cases remained unstable in their sensory behavior. In our opinion combinations of 4 sensopathological prinicples allow a better description of the clinical findings in cases showing a more complicated state. The 4 prinicples are 1. haziness of cortical connections, 2. rivalry of more than one correspondence, 3. horror fusionis, 4. inherited amblyopia -- inborn lack of monocular capacity. Furthermore it can be demonstrated that the four prinicples match with recent findings in neurophysiology and neuroanatomy.", "contents": "[Subnormal binocular vision -- principles for the classification of microstrabismus (author's transl)]. The studies of several hundred case records, most of them carefully observed for years, show that a simple definition of microstrabismus is not sufficient. Contrary to usual opinions about correspondence problems 7% of these cases were even found to be corresponding normally. One third of the cases remained unstable in their sensory behavior. In our opinion combinations of 4 sensopathological prinicples allow a better description of the clinical findings in cases showing a more complicated state. The 4 prinicples are 1. haziness of cortical connections, 2. rivalry of more than one correspondence, 3. horror fusionis, 4. inherited amblyopia -- inborn lack of monocular capacity. Furthermore it can be demonstrated that the four prinicples match with recent findings in neurophysiology and neuroanatomy.", "PMID": 979042}
{"id": "pubmed23n0032_11965", "title": "[Fluorescein angiographic picture of sympathetic ophthalmia (author's transl)].", "content": "The fluorescein angiographic picture of sympathetic ophthalmia is described in an instructive case, which first started at the posterior pole. In the acute phase there was a picture of an exudative process with multiple subretinal enlarging hyperfluorecent spots and some dye-pooling. The retinal vasculature was unaffected. In the healing phase a transitory involvement of the optic nerve head was observed. The cicatricial phase was characterized by a coarsening of the pigment pattern of the fundus. In addition to fluorescein angiography the degree of transitory hyperopia was an excellent indicator of the activity of the disease process.", "contents": "[Fluorescein angiographic picture of sympathetic ophthalmia (author's transl)]. The fluorescein angiographic picture of sympathetic ophthalmia is described in an instructive case, which first started at the posterior pole. In the acute phase there was a picture of an exudative process with multiple subretinal enlarging hyperfluorecent spots and some dye-pooling. The retinal vasculature was unaffected. In the healing phase a transitory involvement of the optic nerve head was observed. The cicatricial phase was characterized by a coarsening of the pigment pattern of the fundus. In addition to fluorescein angiography the degree of transitory hyperopia was an excellent indicator of the activity of the disease process.", "PMID": 979043}
{"id": "pubmed23n0032_11966", "title": "[Antilymphocyte-globulin and cytostatic therapy in the case of sympathetic ophthalmia (author's transl)].", "content": "The authors make known a combined immunosuppresive treatment in a case of sympathetic ophthalmia, which could not be healed by preliminary corticosteroid therapy. The authors recommend in addition to a high dosage of corticosteroids an optimal combination of cytostatics and administration of ALG.", "contents": "[Antilymphocyte-globulin and cytostatic therapy in the case of sympathetic ophthalmia (author's transl)]. The authors make known a combined immunosuppresive treatment in a case of sympathetic ophthalmia, which could not be healed by preliminary corticosteroid therapy. The authors recommend in addition to a high dosage of corticosteroids an optimal combination of cytostatics and administration of ALG.", "PMID": 979044}
{"id": "pubmed23n0032_11967", "title": "[The central vitreo-retinal fibroplastic (edematous-fibroplastic) syndrome (author's transl)].", "content": "This term comprises a series of symptoms with a common pathogenesis. The disease begins with disseminated foci of paravascular fibroplasia which are ophthalmoscopically visible as \"stationary reflexes\" (Vodovozev 1963). These foci result in the formation of vitreo-retinal adhesions. A posterior vitreous detachment effects then traction on the retina with subsequent retinal hypoxia, destruction and edema. This edema if often cystoid, macular holes may originate also. Some patients develop degeneration of connective tissue in the central part of the retina, which may proceed intra- or epiretinally. The syndrome complicates cases of uveitis, senile changes of the fundus, diabetic and hypertonic retinopathies and tapetoretinal degenerations as the postoperative course after extraction of cataract or coagulation of retinal detachment. A classification of the different forms of this syndrome is proposed, its clinical picture and its evolution are described.  Possibilities of treatment and experiences gained in it are shown.", "contents": "[The central vitreo-retinal fibroplastic (edematous-fibroplastic) syndrome (author's transl)]. This term comprises a series of symptoms with a common pathogenesis. The disease begins with disseminated foci of paravascular fibroplasia which are ophthalmoscopically visible as \"stationary reflexes\" (Vodovozev 1963). These foci result in the formation of vitreo-retinal adhesions. A posterior vitreous detachment effects then traction on the retina with subsequent retinal hypoxia, destruction and edema. This edema if often cystoid, macular holes may originate also. Some patients develop degeneration of connective tissue in the central part of the retina, which may proceed intra- or epiretinally. The syndrome complicates cases of uveitis, senile changes of the fundus, diabetic and hypertonic retinopathies and tapetoretinal degenerations as the postoperative course after extraction of cataract or coagulation of retinal detachment. A classification of the different forms of this syndrome is proposed, its clinical picture and its evolution are described.  Possibilities of treatment and experiences gained in it are shown.", "PMID": 979045}
{"id": "pubmed23n0032_11968", "title": "[Signs and symptoms of endocrine orbitopathy (author's transl)].", "content": "The typical motility disturbances of endocrine orbitopathy and the methods of examination are described. Particular reference was placed on the active force generation test and the passive forced duction test. The differential diagnosis between endocrine orbitopathy and tumor was described. Occasionally the endocrine infiltrations may lead to retinal folds or to a tumour-like echographic picture.", "contents": "[Signs and symptoms of endocrine orbitopathy (author's transl)]. The typical motility disturbances of endocrine orbitopathy and the methods of examination are described. Particular reference was placed on the active force generation test and the passive forced duction test. The differential diagnosis between endocrine orbitopathy and tumor was described. Occasionally the endocrine infiltrations may lead to retinal folds or to a tumour-like echographic picture.", "PMID": 979046}
{"id": "pubmed23n0032_11969", "title": "[Cell metabolic investigations as a test of vitality of frozen preserved corneas (author's transl)].", "content": "The protective effect of DMSO in cryopreservation (Capella a. Kaufmann) of 36 corneas is demonstrated by estimation of carbon dioxide as well by electronmicroscopical methods.", "contents": "[Cell metabolic investigations as a test of vitality of frozen preserved corneas (author's transl)]. The protective effect of DMSO in cryopreservation (Capella a. Kaufmann) of 36 corneas is demonstrated by estimation of carbon dioxide as well by electronmicroscopical methods.", "PMID": 979047}
{"id": "pubmed23n0032_11970", "title": "[Corneal operations for lessening high anisometropia in children (statistical results of refractive keratoplasty) (author's transl)].", "content": "Sixty four cases of refractive keratoplasty in children are described. The postoperative results are compared with the preoperative values statistically. The correction of the anisometropia by surgical procedures was succesful in the majority of patients. The average postoperative increase in visual acuity was 0.2. The complications following surgery were minimal.", "contents": "[Corneal operations for lessening high anisometropia in children (statistical results of refractive keratoplasty) (author's transl)]. Sixty four cases of refractive keratoplasty in children are described. The postoperative results are compared with the preoperative values statistically. The correction of the anisometropia by surgical procedures was succesful in the majority of patients. The average postoperative increase in visual acuity was 0.2. The complications following surgery were minimal.", "PMID": 979048}
{"id": "pubmed23n0032_11971", "title": "[Objective vision test in very young children (author's transl)].", "content": "By means of the Catford Visual Acuity Apparatus it is possible to determine objectively the visual acuity even in very young children easily and in a very short time; in children aged less than one year in most cases only a binocular later on a monocular acuity could be ascertained. There are almost no difficulties caused by the language. The visual results of 100 patients are given, 63 of which were under 3 years old; 10 children were aged between 5 and 24 months. A control group of 30 patients was also tested with Pfl\u00fcger optotypes, and the results were compared; half of them were as good as, half of them -- mostly slightly -- better than the results gained by the Pfl\u00fcger-test. We can therefore recommend the use of the Catford Apparatus in very young children and in such cases where the visual acuit can be determined only under very difficult conditions. The results are exact and can be assumed to be the same as with Pfl\u00fcger's optotypes. Moreover, the Catford's apparatus may be also used for the testing of aggravants.", "contents": "[Objective vision test in very young children (author's transl)]. By means of the Catford Visual Acuity Apparatus it is possible to determine objectively the visual acuity even in very young children easily and in a very short time; in children aged less than one year in most cases only a binocular later on a monocular acuity could be ascertained. There are almost no difficulties caused by the language. The visual results of 100 patients are given, 63 of which were under 3 years old; 10 children were aged between 5 and 24 months. A control group of 30 patients was also tested with Pfl\u00fcger optotypes, and the results were compared; half of them were as good as, half of them -- mostly slightly -- better than the results gained by the Pfl\u00fcger-test. We can therefore recommend the use of the Catford Apparatus in very young children and in such cases where the visual acuit can be determined only under very difficult conditions. The results are exact and can be assumed to be the same as with Pfl\u00fcger's optotypes. Moreover, the Catford's apparatus may be also used for the testing of aggravants.", "PMID": 979049}
{"id": "pubmed23n0032_11972", "title": "[Intraocular solitary granuloma of toxocara canis (author's transl)].", "content": "5 cases of intraocular Toxocara canis solitary granuloma are demonstrated. The coincidence of two different aspects of a disease: pseudocoloboma of the macula (toxoplasmosis) in the right and a toxocara solitary granuloma in the left eye of the same patient (case 5) perhaps will furnish proof possibility of foetal infection (via placenta) by Toxocara canis.", "contents": "[Intraocular solitary granuloma of toxocara canis (author's transl)]. 5 cases of intraocular Toxocara canis solitary granuloma are demonstrated. The coincidence of two different aspects of a disease: pseudocoloboma of the macula (toxoplasmosis) in the right and a toxocara solitary granuloma in the left eye of the same patient (case 5) perhaps will furnish proof possibility of foetal infection (via placenta) by Toxocara canis.", "PMID": 979050}
{"id": "pubmed23n0032_11973", "title": "[The fellow eye in acute angle-closure glaucoma (author's transl)].", "content": "With 5 years after the acute angle-closure glaucoma of an eye, in the fellow eye the same disease developed in only 15% of cases, but in 42% of cases in spite of the preventive miotic therapy a chronic angle-closure glaucoma developed unnoticed by the patients. Hence preventive iridectomy is suggested in the fellow eye.", "contents": "[The fellow eye in acute angle-closure glaucoma (author's transl)]. With 5 years after the acute angle-closure glaucoma of an eye, in the fellow eye the same disease developed in only 15% of cases, but in 42% of cases in spite of the preventive miotic therapy a chronic angle-closure glaucoma developed unnoticed by the patients. Hence preventive iridectomy is suggested in the fellow eye.", "PMID": 979051}
{"id": "pubmed23n0032_11974", "title": "[Rehabilitation into employability after traumatic loss of an eye (author's transl)].", "content": "After the loss of an eye re-education can become either imperative according to present law or because of medical reasons. The insurer's attention should be drawn to these facts very early, perhaps already by the accident report, to avoid unnecessary long times of incapacity for work.", "contents": "[Rehabilitation into employability after traumatic loss of an eye (author's transl)]. After the loss of an eye re-education can become either imperative according to present law or because of medical reasons. The insurer's attention should be drawn to these facts very early, perhaps already by the accident report, to avoid unnecessary long times of incapacity for work.", "PMID": 979052}
{"id": "pubmed23n0032_11975", "title": "[The use of peracetic acid for desinfection of tonometers (author's transl)].", "content": "0.1% Peracetic acid (Wofasteril) is suited for desinfection of tonometers, because it has a great rapid microbicidal effect and low concentration. Moreover it is very compatible with cornea and conjunctiva.", "contents": "[The use of peracetic acid for desinfection of tonometers (author's transl)]. 0.1% Peracetic acid (Wofasteril) is suited for desinfection of tonometers, because it has a great rapid microbicidal effect and low concentration. Moreover it is very compatible with cornea and conjunctiva.", "PMID": 979053}
{"id": "pubmed23n0032_11976", "title": "The effect of saralasin (1sar-8-ala-angiotensin II) on blood pressure in patients with Cushing's syndrome.", "content": "To investigate the role of the renin angiotensin system in the pathogenesis of hypertension in Cushing's syndrome two patients with hypercorticism were infused with 20 mg saralasin (1-sar-8-ala-angiotensin II) over a period of 30 minutes under constant blood pressue control. In addition, one patient with primary aldosteronism, an established form of mineralocorticoid hypertension, served as control. Neither in the two patients with Cushing's syndrome nor in the patient with primary aldosteronism could a blood pressure lowering effect of saralasin be observed. In the two patients with hypercoritcism both renin activity and plasma aldosterone increased during saralasin infusion. The patient with primary aldosteronism only showed a weak increase in plasma aldosterone concentration.", "contents": "The effect of saralasin (1sar-8-ala-angiotensin II) on blood pressure in patients with Cushing's syndrome. To investigate the role of the renin angiotensin system in the pathogenesis of hypertension in Cushing's syndrome two patients with hypercorticism were infused with 20 mg saralasin (1-sar-8-ala-angiotensin II) over a period of 30 minutes under constant blood pressue control. In addition, one patient with primary aldosteronism, an established form of mineralocorticoid hypertension, served as control. Neither in the two patients with Cushing's syndrome nor in the patient with primary aldosteronism could a blood pressure lowering effect of saralasin be observed. In the two patients with hypercoritcism both renin activity and plasma aldosterone increased during saralasin infusion. The patient with primary aldosteronism only showed a weak increase in plasma aldosterone concentration.", "PMID": 979066}
{"id": "pubmed23n0032_11977", "title": "Clinical correlates of in vitro effect of adriamycin on advanced lung carcinoma.", "content": "The effect of 1.38 X 10(-5) M adriamycin on tritiated uridine incorporation was studied after 3 hours treatment of suspensions of 25 advanced human lung carcinomas in vitro. The results were correlated with the responses to clinical therapy. All tumours which showed an inhibition of uridine incorporation in vitro of more than 40% were also sensitive to clinical treatment with adriamycin.", "contents": "Clinical correlates of in vitro effect of adriamycin on advanced lung carcinoma. The effect of 1.38 X 10(-5) M adriamycin on tritiated uridine incorporation was studied after 3 hours treatment of suspensions of 25 advanced human lung carcinomas in vitro. The results were correlated with the responses to clinical therapy. All tumours which showed an inhibition of uridine incorporation in vitro of more than 40% were also sensitive to clinical treatment with adriamycin.", "PMID": 979067}
{"id": "pubmed23n0032_11978", "title": "[Influence of spirolactone on the myocardial potassium balance following strophanthin in man (author's transl)].", "content": "In order to examine the question, whether improved digitalis tolerance by Spirolactone may be partially a result of antagonism on myocardial potassium balance, 6 patients without clinical signs of heart failure were given 400 mg, 3 were given 200 or 300 mg Spirolactone orally daily and 6 patients received placebo during a 5 to 7 days period. During cardiac catheterization hemodynamics and serum potassium concentrations were determined repeatedly prior to and following intravenous administration of 0,375 to 0,625 mg Strophanthin. Injection of Strophanthin resulted in a significant drop in left ventricular enddiastolic pressure and a rise in dp/dt max. In the control group a significant increase in arterial and coronary sinus potassium concentration was observed. Myocardial potassium balance was definitely negative from the third to the eighth minute. Values in the group receiving Spirolactone did not differ significantly from the placebo group. It is suggested that therapeutic doses of Strophanthin resllt in a loss of potassium from the myocardium by inhibition of the Na+, K+ membrane ATPase not influenced by pretreatment with Spirolactone.", "contents": "[Influence of spirolactone on the myocardial potassium balance following strophanthin in man (author's transl)]. In order to examine the question, whether improved digitalis tolerance by Spirolactone may be partially a result of antagonism on myocardial potassium balance, 6 patients without clinical signs of heart failure were given 400 mg, 3 were given 200 or 300 mg Spirolactone orally daily and 6 patients received placebo during a 5 to 7 days period. During cardiac catheterization hemodynamics and serum potassium concentrations were determined repeatedly prior to and following intravenous administration of 0,375 to 0,625 mg Strophanthin. Injection of Strophanthin resulted in a significant drop in left ventricular enddiastolic pressure and a rise in dp/dt max. In the control group a significant increase in arterial and coronary sinus potassium concentration was observed. Myocardial potassium balance was definitely negative from the third to the eighth minute. Values in the group receiving Spirolactone did not differ significantly from the placebo group. It is suggested that therapeutic doses of Strophanthin resllt in a loss of potassium from the myocardium by inhibition of the Na+, K+ membrane ATPase not influenced by pretreatment with Spirolactone.", "PMID": 979068}
{"id": "pubmed23n0032_11979", "title": "[Fatty acids in 80 brands of edible vegetable oil and in 14 brands of mayonnaise (author's transl)].", "content": "The fatty acid patterns have been defined in 80 commonly used table oils chosen at random and of 14 brands of mayonnaise commercially available in West Germany. In addition, sterols of the various mayonnaises were analysed. The oils could be grouped into 8 different categories: 12 oliver oils, 8 maize oils, 14 soya oils, 5 linseed oils, 22 sunflower oils, 3 safflower oils, 5 groundnut oils, and 8 rapeseed oils. As a result of the analyses, it is in almost every case a question of a pure variety of non-animal oils. The range of the values obtained for the composition of the fatty acids for each variety, which can be considerable, represents a modern complement to previous analyses. The fatty acid pattern of 3 additional commerical table oils suggest an amalgamation with fats of other types, among which are 2 cases of mixture with rapeseed oils. For mayonnaise, soya oil is used almost exclusively as a fat component. The presence of sunflower oil was only confirmed in one brand of mayonnaise. The concentrations of cholesterol which were determined lie between 17.0 and 72.3 mg (average 53 mg) per 100 g mayonnaise, so that even in the case of persons which present an elevated risk of atherosclerosis there can be no objection to the consumption of mayonnaise. The nutritive-physiological significance of these various oils for human nutrition is thoroughly discussed. A possible health hazard is the sale of vegetable oils rich in erucic acid permitted in the West German Republic (proportion of erucic acid in the 8 samples examined: 223-53%). These vegetable oils, as is shown by the additionally indicated trade names with the corresponding supplementary specifications are overwhelmingly labelled as vegetable oil (Pflanzen\u00f6l) or table oil (Speise\u00f6l). The purchase prices indicated and paid by us for the oils examined are subject to considerable fluctuations even for oils of the same quality.", "contents": "[Fatty acids in 80 brands of edible vegetable oil and in 14 brands of mayonnaise (author's transl)]. The fatty acid patterns have been defined in 80 commonly used table oils chosen at random and of 14 brands of mayonnaise commercially available in West Germany. In addition, sterols of the various mayonnaises were analysed. The oils could be grouped into 8 different categories: 12 oliver oils, 8 maize oils, 14 soya oils, 5 linseed oils, 22 sunflower oils, 3 safflower oils, 5 groundnut oils, and 8 rapeseed oils. As a result of the analyses, it is in almost every case a question of a pure variety of non-animal oils. The range of the values obtained for the composition of the fatty acids for each variety, which can be considerable, represents a modern complement to previous analyses. The fatty acid pattern of 3 additional commerical table oils suggest an amalgamation with fats of other types, among which are 2 cases of mixture with rapeseed oils. For mayonnaise, soya oil is used almost exclusively as a fat component. The presence of sunflower oil was only confirmed in one brand of mayonnaise. The concentrations of cholesterol which were determined lie between 17.0 and 72.3 mg (average 53 mg) per 100 g mayonnaise, so that even in the case of persons which present an elevated risk of atherosclerosis there can be no objection to the consumption of mayonnaise. The nutritive-physiological significance of these various oils for human nutrition is thoroughly discussed. A possible health hazard is the sale of vegetable oils rich in erucic acid permitted in the West German Republic (proportion of erucic acid in the 8 samples examined: 223-53%). These vegetable oils, as is shown by the additionally indicated trade names with the corresponding supplementary specifications are overwhelmingly labelled as vegetable oil (Pflanzen\u00f6l) or table oil (Speise\u00f6l). The purchase prices indicated and paid by us for the oils examined are subject to considerable fluctuations even for oils of the same quality.", "PMID": 979069}
{"id": "pubmed23n0032_11980", "title": "[Significance of forced expiratory vital capacity-curves to detect early mechanical alterations in human lungs (author's transl)].", "content": "In persons who have been smoking cigarettes for many years, who have normal conventional lung function tests the semilogarithmical plotting of forced expiratory vital capacity curves (FEVC) shows statistically significant differences in volume changes below 25 per cent of vital capacity when compared to FEVC-curves of normal subjects of comparable age. On the basis of the equal pressure point theory it is suggested that the simple semilogarithmical plotting of FEVC-curves may reveal early mechanical alterations rather than can be shown by spirometry and airway resistance measurements.", "contents": "[Significance of forced expiratory vital capacity-curves to detect early mechanical alterations in human lungs (author's transl)]. In persons who have been smoking cigarettes for many years, who have normal conventional lung function tests the semilogarithmical plotting of forced expiratory vital capacity curves (FEVC) shows statistically significant differences in volume changes below 25 per cent of vital capacity when compared to FEVC-curves of normal subjects of comparable age. On the basis of the equal pressure point theory it is suggested that the simple semilogarithmical plotting of FEVC-curves may reveal early mechanical alterations rather than can be shown by spirometry and airway resistance measurements.", "PMID": 979070}
{"id": "pubmed23n0032_11981", "title": "Serial measurements of total blood volume in patients on maintenance hemodialysis.", "content": "In 10 patients on maintenance dialysis red cell and plasma volumes (PV) were measured before and after dialysis and several times during the next 3 days in order to establish the usual pattern of intravascular variation associated with this treatment and to determine whether there was a correlation with dialysis induced untoward effects. PV decreased from 23.7 +/- 1.5 before to 20.7 +/- 1.5 ml/cm body height immediately after dialysis. Unexpectedly it was even significantly lower 16 h after (20.1 +/- 1.4 ml/cm). Nevertheless, undesired dialysis symptoms had disappeared by that time indicating that they had not been caused by a reduction in PV or that the patients had adapted to it. Changes in PV correlated better with changes in hematocrit (r = 0.89) than with changes in body weight (r = 0.60). Therefore careful determination of the large vessel hematocrit offers a reliable estimation of blood volume variations over short periods.", "contents": "Serial measurements of total blood volume in patients on maintenance hemodialysis. In 10 patients on maintenance dialysis red cell and plasma volumes (PV) were measured before and after dialysis and several times during the next 3 days in order to establish the usual pattern of intravascular variation associated with this treatment and to determine whether there was a correlation with dialysis induced untoward effects. PV decreased from 23.7 +/- 1.5 before to 20.7 +/- 1.5 ml/cm body height immediately after dialysis. Unexpectedly it was even significantly lower 16 h after (20.1 +/- 1.4 ml/cm). Nevertheless, undesired dialysis symptoms had disappeared by that time indicating that they had not been caused by a reduction in PV or that the patients had adapted to it. Changes in PV correlated better with changes in hematocrit (r = 0.89) than with changes in body weight (r = 0.60). Therefore careful determination of the large vessel hematocrit offers a reliable estimation of blood volume variations over short periods.", "PMID": 979071}
{"id": "pubmed23n0032_11982", "title": "[Incorporation of 4-14C-labeled cholesterol into the cholesterol ester fractions of plasma lipoproteins in patients with cirrhosis of the liver (author's transl)].", "content": "In vivo incorporation studies with 4-14C-cholesterol were performed in five patients with cirrhosis of the liver. All patients showed low concentrations of total cholesterol, esterified cholesterol, and a low activity of the lecithin: cholesterol acyltransferase activity (LCAT). The pattern of incorporation corresponded to the results known from hypercholesterolemic, normocholesterolemic and hypertriglyceridemic patients. Low cholesterol ester concentrations and low LCAT activity do not modify the pattern of incorporation into the lipoprotein ester fractions.", "contents": "[Incorporation of 4-14C-labeled cholesterol into the cholesterol ester fractions of plasma lipoproteins in patients with cirrhosis of the liver (author's transl)]. In vivo incorporation studies with 4-14C-cholesterol were performed in five patients with cirrhosis of the liver. All patients showed low concentrations of total cholesterol, esterified cholesterol, and a low activity of the lecithin: cholesterol acyltransferase activity (LCAT). The pattern of incorporation corresponded to the results known from hypercholesterolemic, normocholesterolemic and hypertriglyceridemic patients. Low cholesterol ester concentrations and low LCAT activity do not modify the pattern of incorporation into the lipoprotein ester fractions.", "PMID": 979072}
{"id": "pubmed23n0032_11983", "title": "The rationale in the treatment of peptic ulcer disease.", "content": "1) In chronic gastric ulcer bed rest, leaving out smoking and carbenoxolone have increased the rate of healing. 2) In chronic duodenal ulcer it has yet to be shown that any medical measure so far significantly altered the course of the disease. 3) The surgical approach to the treatment of chronic duodenal ulcer is based upon three principles, all of them aiming at reducing the acid-pepsin secretion: a) Removal of the acid secreting mucosa decreases the acid secretory capacity of the stomach; b) Removal of the pyloric antrum eliminates the main source of gastrin, the major known humoral excitant of acid secretion; c) Section of the vagi probably renders the acid secreting cells less responsive to humoral stimulation (Brooks). The major problem, however, which is the prevention of recurrent duodenal ulcer is yet unsolved. There is some hope that the new receptor blocking agent metiamid is the first step, and there is even more hope in that the proceedings of this symposium will show us further steps forward.", "contents": "The rationale in the treatment of peptic ulcer disease. 1) In chronic gastric ulcer bed rest, leaving out smoking and carbenoxolone have increased the rate of healing. 2) In chronic duodenal ulcer it has yet to be shown that any medical measure so far significantly altered the course of the disease. 3) The surgical approach to the treatment of chronic duodenal ulcer is based upon three principles, all of them aiming at reducing the acid-pepsin secretion: a) Removal of the acid secreting mucosa decreases the acid secretory capacity of the stomach; b) Removal of the pyloric antrum eliminates the main source of gastrin, the major known humoral excitant of acid secretion; c) Section of the vagi probably renders the acid secreting cells less responsive to humoral stimulation (Brooks). The major problem, however, which is the prevention of recurrent duodenal ulcer is yet unsolved. There is some hope that the new receptor blocking agent metiamid is the first step, and there is even more hope in that the proceedings of this symposium will show us further steps forward.", "PMID": 979073}
{"id": "pubmed23n0032_11984", "title": "Histamine and peptic ulcer: a prospective study of mucosal histamine concentration in duodenal ulcer patients and in control subjects suffering from various gastrointestinal diseases.", "content": "In a prospective study the histamine content of the mucosa of the body of the stomach was measured in 100 patients consisting of control subjects, patients with duodenal ulcer and patients suffering from various gastrointestinal diseases. The histamine content was found to be 43 mug/g in male control subjects (median) while in duodenal ulcer patients levels attained were significantly lower by about 30 per cent. In all the other groups of patients histamine concentrations in gastric mucosa were found to be \"normal\". Since in most species it is extremely diffcult to alter the mucosal histamine concentration by any form of treatment, the diminished histamine content of the gastric mucosa in patients with duodenal ulcer seems remarkable. Among several possible explanations offered for this finding we think the most likely is that histamine release is increased in duodenal ulcer disease.", "contents": "Histamine and peptic ulcer: a prospective study of mucosal histamine concentration in duodenal ulcer patients and in control subjects suffering from various gastrointestinal diseases. In a prospective study the histamine content of the mucosa of the body of the stomach was measured in 100 patients consisting of control subjects, patients with duodenal ulcer and patients suffering from various gastrointestinal diseases. The histamine content was found to be 43 mug/g in male control subjects (median) while in duodenal ulcer patients levels attained were significantly lower by about 30 per cent. In all the other groups of patients histamine concentrations in gastric mucosa were found to be \"normal\". Since in most species it is extremely diffcult to alter the mucosal histamine concentration by any form of treatment, the diminished histamine content of the gastric mucosa in patients with duodenal ulcer seems remarkable. Among several possible explanations offered for this finding we think the most likely is that histamine release is increased in duodenal ulcer disease.", "PMID": 979074}
{"id": "pubmed23n0032_11985", "title": "The role of gastrin in duodenal ulcer surgery.", "content": "The role of gastrin and of serum gastrin analysis in duodenal ulcer disease and duodenal ulcer surgery is analysed. As far as etiology and pathogenesis are concerned up to now gastrin has never been shown to play a significant role. Neither does it provide any diagnostic help in the typical duodenal ulcer disease (but it will allow for diagnosis of the retained antrum after Billroth II resection and of the Zollinger Ellison syndrome). Gastrin determination therefore is not helpful in the choice of the correct operative procedure for the ulcer disease. In today's clinical practice its major role consists in the control of surgical results. This is illustrated by a prospective randomized study on proximal selective vagotomy with and without pyloroplasty. In these patients serum gastrin analysis has shown that the omission of pyloroplasty is not followed by antral stasis. It furthermore always exhibits the typicel vagotomy profile, although vagotomy is incomplete in the 2-DODG-test.", "contents": "The role of gastrin in duodenal ulcer surgery. The role of gastrin and of serum gastrin analysis in duodenal ulcer disease and duodenal ulcer surgery is analysed. As far as etiology and pathogenesis are concerned up to now gastrin has never been shown to play a significant role. Neither does it provide any diagnostic help in the typical duodenal ulcer disease (but it will allow for diagnosis of the retained antrum after Billroth II resection and of the Zollinger Ellison syndrome). Gastrin determination therefore is not helpful in the choice of the correct operative procedure for the ulcer disease. In today's clinical practice its major role consists in the control of surgical results. This is illustrated by a prospective randomized study on proximal selective vagotomy with and without pyloroplasty. In these patients serum gastrin analysis has shown that the omission of pyloroplasty is not followed by antral stasis. It furthermore always exhibits the typicel vagotomy profile, although vagotomy is incomplete in the 2-DODG-test.", "PMID": 979075}
{"id": "pubmed23n0032_11986", "title": "[Enzyme activities of beta-hexosaminidase in urine from patients with renal diseases (author's transl)].", "content": "Enzymatic activities of beta-hexosaminidase and beta-galactosidase were determined in 24 h urine specimens from patients with renal diseases, from individuals with essential hypertension and patients with other diseases. Patients suffering from various renal diseases had significantly higher activities of the beta-hexosaminidase than individuals with essential hypertension or other diseases. However for beta-galactosidase only the mean value of the enzymatic activities was elevated. The possible mechanisms causing an increase of these enzymes in 24 h urine samples are discussed.", "contents": "[Enzyme activities of beta-hexosaminidase in urine from patients with renal diseases (author's transl)]. Enzymatic activities of beta-hexosaminidase and beta-galactosidase were determined in 24 h urine specimens from patients with renal diseases, from individuals with essential hypertension and patients with other diseases. Patients suffering from various renal diseases had significantly higher activities of the beta-hexosaminidase than individuals with essential hypertension or other diseases. However for beta-galactosidase only the mean value of the enzymatic activities was elevated. The possible mechanisms causing an increase of these enzymes in 24 h urine samples are discussed.", "PMID": 979076}
{"id": "pubmed23n0032_11987", "title": "Molecular basis of a control mechanism of DNA synthesis in mammalian cells.", "content": "Personal observations made on the model of isoproterenol-stimulated DNA synthesis have pointed out the following: 1) cell hypertrophy precedes constantly the onset of DNA synthesis; 2) the length of the G1 phase is mass-dependent; 3) accumulation of ribosomes is needed for cell progress through G1; 4) ribosomal protein synthesis is involved in cell growth activation. These results together with a consideration of the pertinent literature allow us to formulate a hypothesis on the control of cell division in mammalian cells. DNA synthesis might be the terminal event in a chain of metabolic processes whereby a cell adjusts itself to increased functional demands (Increased Functional Demand Hypothesis). The main points of this model are the following: the interaction of the extracellular effector on the target cells first activates the pre-existent protein-synthesizing apparatus of the cell, which in turn brings about the activation of the \"translation-transcription connecting mechanism\" whereby the cells adjusts itself to an increased need for protein synthesis. Such a mechanism is characterized by cytoplasmic signals arising from the protein-synthesizing apparatus of the cell which reach the nucleus and call forth a messenger RNA for ribosomal proteins. The latter, once synthesized, protect the nascent ribosomal RNA from nuclease attack, resulting in an accumulation of ribosomes in the cytoplasm. Once the ribosomes have reached a \"critical amount\", the cell is triggered to enter DNA synthesis. As a link between the enhanced ribosomal RNA synthesis and DNA synthesis a reduction in the capacity of the ribonucleotide pool as source of DNA precursors has been suggested.", "contents": "Molecular basis of a control mechanism of DNA synthesis in mammalian cells. Personal observations made on the model of isoproterenol-stimulated DNA synthesis have pointed out the following: 1) cell hypertrophy precedes constantly the onset of DNA synthesis; 2) the length of the G1 phase is mass-dependent; 3) accumulation of ribosomes is needed for cell progress through G1; 4) ribosomal protein synthesis is involved in cell growth activation. These results together with a consideration of the pertinent literature allow us to formulate a hypothesis on the control of cell division in mammalian cells. DNA synthesis might be the terminal event in a chain of metabolic processes whereby a cell adjusts itself to increased functional demands (Increased Functional Demand Hypothesis). The main points of this model are the following: the interaction of the extracellular effector on the target cells first activates the pre-existent protein-synthesizing apparatus of the cell, which in turn brings about the activation of the \"translation-transcription connecting mechanism\" whereby the cells adjusts itself to an increased need for protein synthesis. Such a mechanism is characterized by cytoplasmic signals arising from the protein-synthesizing apparatus of the cell which reach the nucleus and call forth a messenger RNA for ribosomal proteins. The latter, once synthesized, protect the nascent ribosomal RNA from nuclease attack, resulting in an accumulation of ribosomes in the cytoplasm. Once the ribosomes have reached a \"critical amount\", the cell is triggered to enter DNA synthesis. As a link between the enhanced ribosomal RNA synthesis and DNA synthesis a reduction in the capacity of the ribonucleotide pool as source of DNA precursors has been suggested.", "PMID": 979077}
{"id": "pubmed23n0032_11988", "title": "Coagulation studies and platelet function after somatostatin infusion.", "content": "The effect of short- and long-term somatostatin (GIF) administration on haemostatic function in man was investigated. The dosage programme applied in this study was 250 mug GIF as a bolus injection and 250 mug GIF/h by way of infusion. In five healthy volunteers a short-term (3h) treatment resulted in a statistically significant drop of platelet count and impairment of platelet aggregation at the end of infusion. However, these changes were within the physiologically normal range and disappeared after two hours on all subjects. Other parameters such as bleeding time, thromboplastin and partial thromboplastin time, fibrinogen, fibrin/fibrinogen split products, plasma factor XIII, ethanol gelation test were not affected. In two patients with gastric haemorrhage and persistent amylasaemia a 67 or 120-h treatment induced no remarkable haemostatic defect. By contrast, peptic ulcer bleeding in one patient stopped 60 min after starting the GIF infusion. These studies indicated that somatostatin administration in man at the dosage programme used neither results in clinical evidence indicating bleeding tendency nor does it influence laboratory parameters in an apparent way.", "contents": "Coagulation studies and platelet function after somatostatin infusion. The effect of short- and long-term somatostatin (GIF) administration on haemostatic function in man was investigated. The dosage programme applied in this study was 250 mug GIF as a bolus injection and 250 mug GIF/h by way of infusion. In five healthy volunteers a short-term (3h) treatment resulted in a statistically significant drop of platelet count and impairment of platelet aggregation at the end of infusion. However, these changes were within the physiologically normal range and disappeared after two hours on all subjects. Other parameters such as bleeding time, thromboplastin and partial thromboplastin time, fibrinogen, fibrin/fibrinogen split products, plasma factor XIII, ethanol gelation test were not affected. In two patients with gastric haemorrhage and persistent amylasaemia a 67 or 120-h treatment induced no remarkable haemostatic defect. By contrast, peptic ulcer bleeding in one patient stopped 60 min after starting the GIF infusion. These studies indicated that somatostatin administration in man at the dosage programme used neither results in clinical evidence indicating bleeding tendency nor does it influence laboratory parameters in an apparent way.", "PMID": 979078}
{"id": "pubmed23n0032_11989", "title": "[Successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)].", "content": "Continuous plasmapheresis with the IBM blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. The patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. Although 633 mug thyroxine (T4) and 13.6 mug triiodothyronine (T3) were removed with the 51 plasma, no fall occurred in total serum T4 and T3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascular space. Though total T4 and T3 did not change, there was a significant fall in free thyroid hormone levels documented by reduction of T3-uptake test and particularily of urine T4 and T3 excretion. These findings show that the elevation of thyroid hormone binding sites with donor plasma is even more important than the removal of thyroid hormones.", "contents": "[Successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)]. Continuous plasmapheresis with the IBM blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. The patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. Although 633 mug thyroxine (T4) and 13.6 mug triiodothyronine (T3) were removed with the 51 plasma, no fall occurred in total serum T4 and T3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascular space. Though total T4 and T3 did not change, there was a significant fall in free thyroid hormone levels documented by reduction of T3-uptake test and particularily of urine T4 and T3 excretion. These findings show that the elevation of thyroid hormone binding sites with donor plasma is even more important than the removal of thyroid hormones.", "PMID": 979079}
{"id": "pubmed23n0032_11990", "title": "[Lymphocyte stimulation of healthy probands with a \"Hodgkin's HL-A type\" (author's transl)].", "content": "Reported correlations between HL-A type and Hodgkin's disease suggest that genetic factors as HL-A associated immune response genes may play a role in the pathogenesis of Hodgkin's disease. Assuming that healthy individuals having a Hodgkin HL-A constellation may demonstrate impaired immune function mitogen stimulation and mixed lymphocyte reaction were carried out. However, lymphocytes of 12 persons with a Hodgkin-HL-A type did not show any abnormality in the in vitro tests when compared to a control group.", "contents": "[Lymphocyte stimulation of healthy probands with a \"Hodgkin's HL-A type\" (author's transl)]. Reported correlations between HL-A type and Hodgkin's disease suggest that genetic factors as HL-A associated immune response genes may play a role in the pathogenesis of Hodgkin's disease. Assuming that healthy individuals having a Hodgkin HL-A constellation may demonstrate impaired immune function mitogen stimulation and mixed lymphocyte reaction were carried out. However, lymphocytes of 12 persons with a Hodgkin-HL-A type did not show any abnormality in the in vitro tests when compared to a control group.", "PMID": 979080}
{"id": "pubmed23n0032_11991", "title": "[Tolerance of +Gz acceleration by pilots with tendencies to hypotensive reactions in hot climate conditions].", "content": "During acrobatic flying the acceleration tolerance of healthy and hypotensive pilots was studied under hot climate conditions. From the professional point of view pilots were very similar. The tolerance was determined on the basis of indirect indices derived from flight accelerograms (maximum and median acceleration values, number of peaks over 4 g. time of flying). The maximum value and the number of peaks of acceleration (+Gz) over 4 g can be recommended as practically indicative of the tolerance. The observations showed an adverse effect of initial hypotension occurring in hot climate on the acceleration tolerance. As compared with the controls, the tolerance decline in 12 hypotensive pilots was I.I. g and 0.6 g of the maximum and median values, respectively.", "contents": "[Tolerance of +Gz acceleration by pilots with tendencies to hypotensive reactions in hot climate conditions]. During acrobatic flying the acceleration tolerance of healthy and hypotensive pilots was studied under hot climate conditions. From the professional point of view pilots were very similar. The tolerance was determined on the basis of indirect indices derived from flight accelerograms (maximum and median acceleration values, number of peaks over 4 g. time of flying). The maximum value and the number of peaks of acceleration (+Gz) over 4 g can be recommended as practically indicative of the tolerance. The observations showed an adverse effect of initial hypotension occurring in hot climate on the acceleration tolerance. As compared with the controls, the tolerance decline in 12 hypotensive pilots was I.I. g and 0.6 g of the maximum and median values, respectively.", "PMID": 979103}
{"id": "pubmed23n0032_11992", "title": "[Human reactions to prolonged centripetal accelerations (+Gz) of small magnitudes].", "content": "The effect of centripetal acceleration (+Gz) of low values (0.5-0.6 g) applied for four days against the background of relative hypokinesia was investigated. The peripheral and intracranial circulations, equilibrium function and morphological composition of the capillary blood were examined. During the first three centrifugation days the hemodynamic state differed insignificantly from the initial level. On the fourth day signs of cardiovascular deconditioning were found. This was shown by the orthostatic test. Changes in the peripheral blood indicated a moderate stress-reaction that persisted throughout the entire experiment. Equilibrium changes that were observed on the first centrifugation day regressed and the post-test function did not essentially differ from the initial level. The experimental results give evidence that inertia forces can be used to diminish the unfavorable effects of hypokinesia.", "contents": "[Human reactions to prolonged centripetal accelerations (+Gz) of small magnitudes]. The effect of centripetal acceleration (+Gz) of low values (0.5-0.6 g) applied for four days against the background of relative hypokinesia was investigated. The peripheral and intracranial circulations, equilibrium function and morphological composition of the capillary blood were examined. During the first three centrifugation days the hemodynamic state differed insignificantly from the initial level. On the fourth day signs of cardiovascular deconditioning were found. This was shown by the orthostatic test. Changes in the peripheral blood indicated a moderate stress-reaction that persisted throughout the entire experiment. Equilibrium changes that were observed on the first centrifugation day regressed and the post-test function did not essentially differ from the initial level. The experimental results give evidence that inertia forces can be used to diminish the unfavorable effects of hypokinesia.", "PMID": 979104}
{"id": "pubmed23n0032_11993", "title": "[Effect of systematic gravitational forces on the external respiration function in conditions of prolonged immersion].", "content": "Test subjects were exposed to 13-day water immersion per se or combined with centrifugation. The immersion did not influence the state of ventilation and gas exchange at rest, diminished significantly the functional capabilities of external respiration. An exposure of the test subjects to acceleration during the second half of water immersion resulted in the normalization of the functional reserves of external respiration. This may be a consequence of an increase in the total physical tone of the body.", "contents": "[Effect of systematic gravitational forces on the external respiration function in conditions of prolonged immersion]. Test subjects were exposed to 13-day water immersion per se or combined with centrifugation. The immersion did not influence the state of ventilation and gas exchange at rest, diminished significantly the functional capabilities of external respiration. An exposure of the test subjects to acceleration during the second half of water immersion resulted in the normalization of the functional reserves of external respiration. This may be a consequence of an increase in the total physical tone of the body.", "PMID": 979105}
{"id": "pubmed23n0032_11994", "title": "[Reactions of the human cardiovascular system to orthostasis following high altitude adaptation].", "content": "Orthostatic tolerance of 18 healthy test subjects (three groups of 6 persons each) who were exposed to an altitude of 2200 m for 12 days and 3200 m for 12 days was studied. The first group subjects remained in bed with minimized motor activity. The second group test subjects rested in bed and twice a day performed physical exercises with energy expenditures of 300 kcal/day. The third group subjects led a normal mode of life. The studies demonstrated that a prolonged restriction of motor activity and a decrease in the blood hydrostatic pressure of healthy test subjects in highlands resulted in their diminished orthostatic tolerance. Physical exercises proved ineffective in that case. The subjects who led a normal mode of life showed increased cardiovascular reactions to orthostatic tests.", "contents": "[Reactions of the human cardiovascular system to orthostasis following high altitude adaptation]. Orthostatic tolerance of 18 healthy test subjects (three groups of 6 persons each) who were exposed to an altitude of 2200 m for 12 days and 3200 m for 12 days was studied. The first group subjects remained in bed with minimized motor activity. The second group test subjects rested in bed and twice a day performed physical exercises with energy expenditures of 300 kcal/day. The third group subjects led a normal mode of life. The studies demonstrated that a prolonged restriction of motor activity and a decrease in the blood hydrostatic pressure of healthy test subjects in highlands resulted in their diminished orthostatic tolerance. Physical exercises proved ineffective in that case. The subjects who led a normal mode of life showed increased cardiovascular reactions to orthostatic tests.", "PMID": 979106}
{"id": "pubmed23n0032_11995", "title": "[Protein and ribonucleic acid metabolism in the central nervous system of rats during space flight in the \"Kosmos-605\" satellite].", "content": "On the 2nd postflight day the activity of neutral protamine peptide hydrolase of different compartments of the rat brain did not differ from the control level. With respect to the protein and RNA content and concentration motoneurons of anterior horns of the spinal cord and their glial-cells-satellites of rats exposed to the ground-based synchronous experiment did not differ from those of vivarium controls, except cells of the supraoptic nucleus. That was found on the 2nd and 27th postflight days. On the 2nd postflight day the protein and RNA concentration in neurons decreased and the protein concentration and content in gliocytes lowered; the RNA concentration and content in Purkinje cells of the cerebellum became reduced. On the 27th postflight day the RNA concentration in neurons of the supraoptic nucleus remained diminished whereas the protein content increased; in spinal motoneurons the protein concentration decreased and in adjacent gliocytes the protein concentration and content lowered. All the above changes made no more than 15% of the control values.", "contents": "[Protein and ribonucleic acid metabolism in the central nervous system of rats during space flight in the \"Kosmos-605\" satellite]. On the 2nd postflight day the activity of neutral protamine peptide hydrolase of different compartments of the rat brain did not differ from the control level. With respect to the protein and RNA content and concentration motoneurons of anterior horns of the spinal cord and their glial-cells-satellites of rats exposed to the ground-based synchronous experiment did not differ from those of vivarium controls, except cells of the supraoptic nucleus. That was found on the 2nd and 27th postflight days. On the 2nd postflight day the protein and RNA concentration in neurons decreased and the protein concentration and content in gliocytes lowered; the RNA concentration and content in Purkinje cells of the cerebellum became reduced. On the 27th postflight day the RNA concentration in neurons of the supraoptic nucleus remained diminished whereas the protein content increased; in spinal motoneurons the protein concentration decreased and in adjacent gliocytes the protein concentration and content lowered. All the above changes made no more than 15% of the control values.", "PMID": 979101}
{"id": "pubmed23n0032_11996", "title": "[Morphological effects in rats following a 22-day space flight].", "content": "A morphological examination of 27 rats flown onboard the biosatellite and sacrificed on the 1st-2nd and 26-27th postflight days demonstrated no significant changes in the structural organization of the vital organs and systems of the animal body. It was, however, found that the space exposure induced morphologically detectable changes in the musculo-skeletal system, hemo- and lymphopoiesis, hypothalamic-pituitary-adrenal system and the juxtaglomerular apparatus of the kidneys. The changes were reversible and nonspecific, and could be seen in animals exposed to ground-based hypokinetic and other stress experiments. Postflight the animals developed some reactions that were similar to those in humans. This helps to identify the morphological substrate of certain changes in the human body and to investigate their pathogenesis.", "contents": "[Morphological effects in rats following a 22-day space flight]. A morphological examination of 27 rats flown onboard the biosatellite and sacrificed on the 1st-2nd and 26-27th postflight days demonstrated no significant changes in the structural organization of the vital organs and systems of the animal body. It was, however, found that the space exposure induced morphologically detectable changes in the musculo-skeletal system, hemo- and lymphopoiesis, hypothalamic-pituitary-adrenal system and the juxtaglomerular apparatus of the kidneys. The changes were reversible and nonspecific, and could be seen in animals exposed to ground-based hypokinetic and other stress experiments. Postflight the animals developed some reactions that were similar to those in humans. This helps to identify the morphological substrate of certain changes in the human body and to investigate their pathogenesis.", "PMID": 979102}
{"id": "pubmed23n0032_11997", "title": "[Quantitative evaluation of ultrastructural changes in the rat myocardium during prolonged hypokinesia].", "content": "It was morphometrically shown that during 120-day hypokinesia chromatin redistribution was similar in nuclei of myocardial cells of the ventricles of the rat heart. Quantitative rearrangements in the mitochondrial apparatus of cells were of different pattern. On the 14th hypokinetic day the number of mitochondria increased and their size decreased; on the 30th day their size increased and their number decreased; on the 45 and 60th day the number and size of mitochondria returned to the normal; on the 120th hypokinetic day the number and size of mitochondria were higher than the control. Throughout the 120-day experimental the ratio of the total area of mitochondria to the total area of myofibers varied periodically and by the end of the experimental the mitochondrial area increased. Thus, it is obvious that by the 120th hypokinetic day myocardial changes increased indicating a new level of cardiac activity during prolonged hypokinesia. Quantitative changes in the organelles of the myocardium of the left ventricle were more significant than those of the right ventricle during the exposure.", "contents": "[Quantitative evaluation of ultrastructural changes in the rat myocardium during prolonged hypokinesia]. It was morphometrically shown that during 120-day hypokinesia chromatin redistribution was similar in nuclei of myocardial cells of the ventricles of the rat heart. Quantitative rearrangements in the mitochondrial apparatus of cells were of different pattern. On the 14th hypokinetic day the number of mitochondria increased and their size decreased; on the 30th day their size increased and their number decreased; on the 45 and 60th day the number and size of mitochondria returned to the normal; on the 120th hypokinetic day the number and size of mitochondria were higher than the control. Throughout the 120-day experimental the ratio of the total area of mitochondria to the total area of myofibers varied periodically and by the end of the experimental the mitochondrial area increased. Thus, it is obvious that by the 120th hypokinetic day myocardial changes increased indicating a new level of cardiac activity during prolonged hypokinesia. Quantitative changes in the organelles of the myocardium of the left ventricle were more significant than those of the right ventricle during the exposure.", "PMID": 979107}
{"id": "pubmed23n0032_11998", "title": "[Effect of nerobol on the water-electrolyte balance and kidney function in man during 120-day hypokinesia].", "content": "The influence of anabolic steroids on the fluid-electrolyte metabolism and renal function of man was studied during a prolonged bed rest experiment. Seven test subjects were kept under surveillance. Three test subjects were given nerobol and four subjects served as controls. The fluid-electrolyte metabolism and the osmoregulatory function of the kidneys were studied. Nerobol induced a decreased excretion of sodium, potassium, calcium, chlorides and osmotically active substances. Nerobol produced the most pronounced effect on the calcium and potassium metabolism. The effect of the drug used during the prolonged bed rest experiment depended on the time and dosage as well as on the state of fluid-eletrolyte metabolism and the renal function.", "contents": "[Effect of nerobol on the water-electrolyte balance and kidney function in man during 120-day hypokinesia]. The influence of anabolic steroids on the fluid-electrolyte metabolism and renal function of man was studied during a prolonged bed rest experiment. Seven test subjects were kept under surveillance. Three test subjects were given nerobol and four subjects served as controls. The fluid-electrolyte metabolism and the osmoregulatory function of the kidneys were studied. Nerobol induced a decreased excretion of sodium, potassium, calcium, chlorides and osmotically active substances. Nerobol produced the most pronounced effect on the calcium and potassium metabolism. The effect of the drug used during the prolonged bed rest experiment depended on the time and dosage as well as on the state of fluid-eletrolyte metabolism and the renal function.", "PMID": 979108}
{"id": "pubmed23n0032_11999", "title": "[Diagnostic value of the free amino acid content in blood plasma during human dietary protein deificiency].", "content": "The influence of alimentary protein deficiency on the content of free amino acids in the blood plasma of man was studied. Changes in the value and arginine concentration that correlated with alterations in the protein content in the diet were the most consistent. The relationship can be used in the biochemical test to detect early stages of protein dificiency in man.", "contents": "[Diagnostic value of the free amino acid content in blood plasma during human dietary protein deificiency]. The influence of alimentary protein deficiency on the content of free amino acids in the blood plasma of man was studied. Changes in the value and arginine concentration that correlated with alterations in the protein content in the diet were the most consistent. The relationship can be used in the biochemical test to detect early stages of protein dificiency in man.", "PMID": 979109}
{"id": "pubmed23n0032_12000", "title": "[Human calorimetry in sealed hermetic enclosures].", "content": "The paper gives the results of an experimental assessment of personal biocalorimeter and calorimetric garment. The paper is addressed to biologists, physiologists and physicians investigating heat exchange and thermal regulation of the human body as well as to thephysicists developing calorimetric theories and methods. It presents theoretical concepts underlying direct calorimetry of a suited man and contains a block-diagram of the biocalorimeter that was used to examine energy parameters of the human body, including metabolic rate, heat production, heat release and heat balance. The data obtained were statistically treated and heat effects were characterized by empiric expressions. It was for the first time in medical practice that the personal calorimeter and calorimetric garment made it possible to accumulate information on the thermal state of the human body. This information helps to diagnose the health condition of man, to standardise environmental parameters in aviation, space and such like suits and to automatize their maintenance in experimental and real life situations.", "contents": "[Human calorimetry in sealed hermetic enclosures]. The paper gives the results of an experimental assessment of personal biocalorimeter and calorimetric garment. The paper is addressed to biologists, physiologists and physicians investigating heat exchange and thermal regulation of the human body as well as to thephysicists developing calorimetric theories and methods. It presents theoretical concepts underlying direct calorimetry of a suited man and contains a block-diagram of the biocalorimeter that was used to examine energy parameters of the human body, including metabolic rate, heat production, heat release and heat balance. The data obtained were statistically treated and heat effects were characterized by empiric expressions. It was for the first time in medical practice that the personal calorimeter and calorimetric garment made it possible to accumulate information on the thermal state of the human body. This information helps to diagnose the health condition of man, to standardise environmental parameters in aviation, space and such like suits and to automatize their maintenance in experimental and real life situations.", "PMID": 979110}
{"id": "pubmed23n0032_12001", "title": "[Hegglin's syndrome in flight personnel with atherosclerotic coronary cardiosclerosis and its clinical evaluation].", "content": "In order to identify the frequency and degree of the Haggline syndrome, 954 polycardiograms recorded in 241 patients, aged 39-55, with moderate atherosclerotic coronary cardiosclerosis were studied. Certain deviations in the mechanical work of the heart were noted. Elongation of the electric systole was found in 16% and that of the electro-mechanical in 21.4% of the subjects. This suggested metabolism disorders in the heart and reduction of its contractile capacity. The study made it possible to identify early abnormalities in the mechanical work of the heart that are very important to prevent further adverse developments.", "contents": "[Hegglin's syndrome in flight personnel with atherosclerotic coronary cardiosclerosis and its clinical evaluation]. In order to identify the frequency and degree of the Haggline syndrome, 954 polycardiograms recorded in 241 patients, aged 39-55, with moderate atherosclerotic coronary cardiosclerosis were studied. Certain deviations in the mechanical work of the heart were noted. Elongation of the electric systole was found in 16% and that of the electro-mechanical in 21.4% of the subjects. This suggested metabolism disorders in the heart and reduction of its contractile capacity. The study made it possible to identify early abnormalities in the mechanical work of the heart that are very important to prevent further adverse developments.", "PMID": 979111}
{"id": "pubmed23n0032_12002", "title": "[Reactions of the cardio-respiratory system of cosmonauts to dosed physical loads following 30- and 63-day flights in orbital station \"Salyut-4\"].", "content": "Cardiorespiratory responses of Salyut-4 crewmembers to exercises of 600 kgm/min performed for 7 min on a bicycle ergometer in the supine position were studied. It was found that the 30- and 63-day flights induced a certain decrease in the body response which included a less economic functioning of the cardiorespiratory system during exercise. The degree of postflight changes of the 30- and 63-day crewmembers was similar. At the same time the cardiorespiratory system of the 63-day crewmembers returned to the preflight level more rapidly than that of the 30-day cosmonauts.", "contents": "[Reactions of the cardio-respiratory system of cosmonauts to dosed physical loads following 30- and 63-day flights in orbital station \"Salyut-4\"]. Cardiorespiratory responses of Salyut-4 crewmembers to exercises of 600 kgm/min performed for 7 min on a bicycle ergometer in the supine position were studied. It was found that the 30- and 63-day flights induced a certain decrease in the body response which included a less economic functioning of the cardiorespiratory system during exercise. The degree of postflight changes of the 30- and 63-day crewmembers was similar. At the same time the cardiorespiratory system of the 63-day crewmembers returned to the preflight level more rapidly than that of the 30-day cosmonauts.", "PMID": 979117}
{"id": "pubmed23n0032_12003", "title": "[Sonic booms--new environmental factor (bases for standardization)].", "content": "The paper discusses physical, psychophysiological and social aspects of a new type of sound effects--sonic shocks of supersonic vehicles. An up-to-date approach to the standardization of environmental effects should include a study of human physiological  responses, comparison of the results of psychophysiological scaling with the available standards and prediction based on sociological questionnaires. This kind of standardization is illustrated.", "contents": "[Sonic booms--new environmental factor (bases for standardization)]. The paper discusses physical, psychophysiological and social aspects of a new type of sound effects--sonic shocks of supersonic vehicles. An up-to-date approach to the standardization of environmental effects should include a study of human physiological  responses, comparison of the results of psychophysiological scaling with the available standards and prediction based on sociological questionnaires. This kind of standardization is illustrated.", "PMID": 979115}
{"id": "pubmed23n0032_12004", "title": "[Effect of 22-day space flight factors on the state of the sex glands and reproductive capacity of rats].", "content": "The paper presents the results of the morphological examination of tests of the Wistar rats flown for 22 days onboard the biosatellite Cosmos-605. Histological, histochemical, electron microscopic and biometric investigations of sex glands carried out 24-48 hours and 26-27 days postflight show that an exposure of the rats to weightlessness and other space flight factors induced no morphological changes in the spermatogenic tissue or disorders in the spermatogenic process of the rats. The offspring of male rats that were exposed to 22-day weightlessness did not differ from the controls with respect to the number of the newborns, their weight at birth, weight gain during the first postnatal month, resistance to hypoxia, etc.", "contents": "[Effect of 22-day space flight factors on the state of the sex glands and reproductive capacity of rats]. The paper presents the results of the morphological examination of tests of the Wistar rats flown for 22 days onboard the biosatellite Cosmos-605. Histological, histochemical, electron microscopic and biometric investigations of sex glands carried out 24-48 hours and 26-27 days postflight show that an exposure of the rats to weightlessness and other space flight factors induced no morphological changes in the spermatogenic tissue or disorders in the spermatogenic process of the rats. The offspring of male rats that were exposed to 22-day weightlessness did not differ from the controls with respect to the number of the newborns, their weight at birth, weight gain during the first postnatal month, resistance to hypoxia, etc.", "PMID": 979120}
{"id": "pubmed23n0032_12005", "title": "[Orthostatic stability in cosmonauts following 30- and 63-day flights in orbital station \"Salyut-4\"].", "content": "Reactions of the circulation system of cosmonauts to the up and down body movements were studied after 30-day and 63-day flights. All the four cosmonauts showed a decline in the orthostatic tolerance. An improved system of countermeasures against unfavourable effects of weightlessness used in the 63-day flight helped to maintain a better orthostatic tolerance of the crewmembers than that of the 30-day cosmonauts. It is assumed that a rapid increase of orthostaic tolerance postflight indicates an adequate maintenance of functional capabilities of circulation and suggests possible stimulation of its antigravity mechanisms prior to reentry in order to prevent postflight orthostatic disorders. An exposure to weightlessness increased the capacity of circulation to counteract the blood  redistribution in the cranial direction in the head-down position. This capacity became more pronounced and stable with an increase in the flight time.", "contents": "[Orthostatic stability in cosmonauts following 30- and 63-day flights in orbital station \"Salyut-4\"]. Reactions of the circulation system of cosmonauts to the up and down body movements were studied after 30-day and 63-day flights. All the four cosmonauts showed a decline in the orthostatic tolerance. An improved system of countermeasures against unfavourable effects of weightlessness used in the 63-day flight helped to maintain a better orthostatic tolerance of the crewmembers than that of the 30-day cosmonauts. It is assumed that a rapid increase of orthostaic tolerance postflight indicates an adequate maintenance of functional capabilities of circulation and suggests possible stimulation of its antigravity mechanisms prior to reentry in order to prevent postflight orthostatic disorders. An exposure to weightlessness increased the capacity of circulation to counteract the blood  redistribution in the cranial direction in the head-down position. This capacity became more pronounced and stable with an increase in the flight time.", "PMID": 979116}
{"id": "pubmed23n0032_12006", "title": "[Theroretical aspects of the problem of prognosing the state of the human body during space flight].", "content": "The paper presents general principles underlying the construction of mathematical systems used to predict the human health status in space flight. It describes methods of prediction based on the concepts of homeostasis and general adaptation syndrome. The paper discusses applications of methods of statistic approximation and expertise estimates to predict changes in different parameters of the Salyut crewmembers. It gives an evaluation of the accuracy with which variations in the heart rate of the Salyut crewmembers were predicted using a linear model and expertise estimates.", "contents": "[Theroretical aspects of the problem of prognosing the state of the human body during space flight]. The paper presents general principles underlying the construction of mathematical systems used to predict the human health status in space flight. It describes methods of prediction based on the concepts of homeostasis and general adaptation syndrome. The paper discusses applications of methods of statistic approximation and expertise estimates to predict changes in different parameters of the Salyut crewmembers. It gives an evaluation of the accuracy with which variations in the heart rate of the Salyut crewmembers were predicted using a linear model and expertise estimates.", "PMID": 979119}
{"id": "pubmed23n0032_12007", "title": "[Preliminary results of medical research completed during the flight of the second expedition of orbital station \"Salyut-4\"].", "content": "The 63-day flight of cosmonauts P. I. Klimuk and V. I. Sevastyanov onboard the orbital station Salyut-4 has shown that man can well adapt to weightlessness and carry out diverse and intensive activities in the weightless state. Weightlessness effects on the human body may be both direct and indirect. The direct effects include reversal of deformations and mechanical tensions in tissue structures, change in the afferent impulsation from receptor zones reacting to the gravity effect, blood redistribution, disturbance in the function of sensory system. The indirect effects of weightlessness are associated with an unusual environment and unusual conditions of work rest, food and water consumption, etc. In the course of flight the human body adapts itself to the new environment; this is assured by self-regulation of physiological functions aimed at the maintenance of a constant level of vitally important parameters. Human adaptation to the weightless state can be subdivided into two periods: 1) period of adaptive rearrangement and 2) period of relative stabilization. The first period includes a rearrangement of functions and regulatory systems of the body. The second period can be defined as attainment of an intersystem homeostasis in the human body and a relatively stable equilibrium of the body with the environment. Incomplete adaptive reactions in shorter flights, e. g. during the first expedition of the orbital station Salyut-4 (G. M. Grechko, A. A. Gubarev), may be one of the factors responsible for a less favourable development of postflight readaptation. Thus, the most important purpose of the medical monitoring and prediction in prolonged space missions is to determine how complete or incomplete these adaptive reactions are. Relative stabilization can be reached, as a rule, after a 1.5 month exposure to weightlessness. However, this time period is rather relative since it depends on the characteristic features of the human body. The results of medical investigations carried out during and after the 63-day flight demonstrated no changes that could prevent from a further increase in the duration of future space missions.", "contents": "[Preliminary results of medical research completed during the flight of the second expedition of orbital station \"Salyut-4\"]. The 63-day flight of cosmonauts P. I. Klimuk and V. I. Sevastyanov onboard the orbital station Salyut-4 has shown that man can well adapt to weightlessness and carry out diverse and intensive activities in the weightless state. Weightlessness effects on the human body may be both direct and indirect. The direct effects include reversal of deformations and mechanical tensions in tissue structures, change in the afferent impulsation from receptor zones reacting to the gravity effect, blood redistribution, disturbance in the function of sensory system. The indirect effects of weightlessness are associated with an unusual environment and unusual conditions of work rest, food and water consumption, etc. In the course of flight the human body adapts itself to the new environment; this is assured by self-regulation of physiological functions aimed at the maintenance of a constant level of vitally important parameters. Human adaptation to the weightless state can be subdivided into two periods: 1) period of adaptive rearrangement and 2) period of relative stabilization. The first period includes a rearrangement of functions and regulatory systems of the body. The second period can be defined as attainment of an intersystem homeostasis in the human body and a relatively stable equilibrium of the body with the environment. Incomplete adaptive reactions in shorter flights, e. g. during the first expedition of the orbital station Salyut-4 (G. M. Grechko, A. A. Gubarev), may be one of the factors responsible for a less favourable development of postflight readaptation. Thus, the most important purpose of the medical monitoring and prediction in prolonged space missions is to determine how complete or incomplete these adaptive reactions are. Relative stabilization can be reached, as a rule, after a 1.5 month exposure to weightlessness. However, this time period is rather relative since it depends on the characteristic features of the human body. The results of medical investigations carried out during and after the 63-day flight demonstrated no changes that could prevent from a further increase in the duration of future space missions.", "PMID": 979118}
{"id": "pubmed23n0032_12008", "title": "[Duration of maintenance of increased bodily resistance with different regimes of adapatation to oxygen deficiency].", "content": "The altitude resistance of albino rats was studied via measurements of their altitude threshold on the 3rd, 7, 15, 30, 45th days of their step-by-step acclimatization to highlands (Tuya-Ashu pass at an altitude of 3200 m), on the 3rd, 7, 15, 30, 45th days of their nonstep-by-step acclimatization at the Tuya-Ashu pass and on the 3rd, 7, 15, 30, 45th days of their altitude chamber training. The step-by-step acclimatization of animals included their 15-day exposure to an altitude of 2200 m and subsequent stay in lowlands where their altitude threshold was recorded on the 3rd, 10, 20 30, 40, 60, 80th days. The data obtained indicate that step-by-step acclimatization to highlands results in a more significant increase of the altitude resistance of the animal body and a longer maintenance of the resistance on return to lowlands.", "contents": "[Duration of maintenance of increased bodily resistance with different regimes of adapatation to oxygen deficiency]. The altitude resistance of albino rats was studied via measurements of their altitude threshold on the 3rd, 7, 15, 30, 45th days of their step-by-step acclimatization to highlands (Tuya-Ashu pass at an altitude of 3200 m), on the 3rd, 7, 15, 30, 45th days of their nonstep-by-step acclimatization at the Tuya-Ashu pass and on the 3rd, 7, 15, 30, 45th days of their altitude chamber training. The step-by-step acclimatization of animals included their 15-day exposure to an altitude of 2200 m and subsequent stay in lowlands where their altitude threshold was recorded on the 3rd, 10, 20 30, 40, 60, 80th days. The data obtained indicate that step-by-step acclimatization to highlands results in a more significant increase of the altitude resistance of the animal body and a longer maintenance of the resistance on return to lowlands.", "PMID": 979123}
{"id": "pubmed23n0032_12009", "title": "[Optimization of rotatory tests in electronystagmography].", "content": "The paper discusses optimization of rotatory tests as related to the classification of electronystagmograms. It presents criteria of the efficiency of the action on the vestibular analyzer taking into account statistical properties of different classes of electronystagmograms. The paper shows that the methods of variation statistics should be used to determine the category of action. The method described has been applied to identify an optimal action in the case of electronystagmograms of two classes (normal and pathology of a certain type). An action described as a sequence of delta-impulses of an angular acceleration has been obtained. A higher efficiency of the optimal action on the vestibular analyzer has been shown on the basis of a comparative study.", "contents": "[Optimization of rotatory tests in electronystagmography]. The paper discusses optimization of rotatory tests as related to the classification of electronystagmograms. It presents criteria of the efficiency of the action on the vestibular analyzer taking into account statistical properties of different classes of electronystagmograms. The paper shows that the methods of variation statistics should be used to determine the category of action. The method described has been applied to identify an optimal action in the case of electronystagmograms of two classes (normal and pathology of a certain type). An action described as a sequence of delta-impulses of an angular acceleration has been obtained. A higher efficiency of the optimal action on the vestibular analyzer has been shown on the basis of a comparative study.", "PMID": 979121}
{"id": "pubmed23n0032_12010", "title": "[Changes in central and peripheral hemodynamics during prolonged anti-orthostatic hypokinesia as a model of weightlessness].", "content": "Central and peripheral circulation was studied rheographically in 6 test subjects during a 30-day bed rest experiment and in 9 test subjects during a 49-day bed rest. The bed rested subjects were kept in a head-down position at an angle of --4 degrees. At an early stage of the experiments the cardiac output and stroke volume increased, the total peripheral resistance decreased, the tone of major regional vessels decreased and that of small vessels increased. During the 3rd-7th weeks the changes were just the opposite: cardiac output and stroke volume decreased, the total peripheral resistance increased the tone of major regional vessels increased and that of small vessels decreased. At the end of the bed rest experiment the cardiac output and stroke volume increased and the tone of regional vessels changed accordingly. Circulation parameters returned to the pretest level, at least, 3-4 weeks after the end of the best rest experiments.", "contents": "[Changes in central and peripheral hemodynamics during prolonged anti-orthostatic hypokinesia as a model of weightlessness]. Central and peripheral circulation was studied rheographically in 6 test subjects during a 30-day bed rest experiment and in 9 test subjects during a 49-day bed rest. The bed rested subjects were kept in a head-down position at an angle of --4 degrees. At an early stage of the experiments the cardiac output and stroke volume increased, the total peripheral resistance decreased, the tone of major regional vessels decreased and that of small vessels increased. During the 3rd-7th weeks the changes were just the opposite: cardiac output and stroke volume decreased, the total peripheral resistance increased the tone of major regional vessels increased and that of small vessels decreased. At the end of the bed rest experiment the cardiac output and stroke volume increased and the tone of regional vessels changed accordingly. Circulation parameters returned to the pretest level, at least, 3-4 weeks after the end of the best rest experiments.", "PMID": 979122}
{"id": "pubmed23n0032_12011", "title": "The microbiological and parasitological status of laboratory animals from accredited breeders in the United Kingdom.", "content": "The incidence of significant organisms isolated from mice, hamsters, rats, guinea-pigs and rabbits from Accredited Breeders in the United Kingdom (1970-1974) is recorded. An improvement in quality in the case of mice, rats and guinea-pigs as a result of hysterectomy derivation is demonstrated and the importance of the various organisms is discussed.", "contents": "The microbiological and parasitological status of laboratory animals from accredited breeders in the United Kingdom. The incidence of significant organisms isolated from mice, hamsters, rats, guinea-pigs and rabbits from Accredited Breeders in the United Kingdom (1970-1974) is recorded. An improvement in quality in the case of mice, rats and guinea-pigs as a result of hysterectomy derivation is demonstrated and the importance of the various organisms is discussed.", "PMID": 979132}
{"id": "pubmed23n0032_12012", "title": "[Flight staff medical expertise with regard to several forms of myocardial disease].", "content": "Cardiovascular disease occupy a major place among the adication required to disqualify pilots due to visceral injuries. Surveillance of the persons with myocardial lesions of non-coronarogenic (infectious, toxic, allergic) genesis suggests that disorders of the myocardial function may be stable, irreversible in some cases and transient in others. The expertise evaluation should take into consideration the pattern and severity of the clinical picture and the status of functional capabilities of the cardiovascular system. The pilots with transient or firstly detected disturbances of myocardial excitability and conduction should be properly treated and released for 1.5-3 months from flying activites. After complete recovery confirmed by a special examination in the hospital they may continue flying.", "contents": "[Flight staff medical expertise with regard to several forms of myocardial disease]. Cardiovascular disease occupy a major place among the adication required to disqualify pilots due to visceral injuries. Surveillance of the persons with myocardial lesions of non-coronarogenic (infectious, toxic, allergic) genesis suggests that disorders of the myocardial function may be stable, irreversible in some cases and transient in others. The expertise evaluation should take into consideration the pattern and severity of the clinical picture and the status of functional capabilities of the cardiovascular system. The pilots with transient or firstly detected disturbances of myocardial excitability and conduction should be properly treated and released for 1.5-3 months from flying activites. After complete recovery confirmed by a special examination in the hospital they may continue flying.", "PMID": 979126}
{"id": "pubmed23n0032_12013", "title": "Erythrocyte basophilic stippling in the Mongolian gerbil.", "content": "Basophilic stippling of the circulating erythrocytes is characteristic of the Mongolian gerbil. Its enzymatic digestion along with the concomitant removal of diffuse erythrocytic polychromasia by the action of ribonuclease demonstrates that it represents microscopically visible aggregates of cytoplasmic ribonucleoprotein, presumably of ribosomal origin. Up to 40% of the total circulating erythrocytes may be stippled in foetal and newborn animals. There is then a progressive decline in incidence until adult levels are attained at least by 20 weeks of age. The bone marrow of the adult gerbil contains a higher proportion of stippled red cells than the circulating blood. The stippling can be either coarse or fine and observable in both polychromatophilic and orthochromic cells. It is suggested that erythrocytes with basophilic stippling are relatively immature red cells still demonstrating remnants of cytoplasmic ribonucleoprotein.", "contents": "Erythrocyte basophilic stippling in the Mongolian gerbil. Basophilic stippling of the circulating erythrocytes is characteristic of the Mongolian gerbil. Its enzymatic digestion along with the concomitant removal of diffuse erythrocytic polychromasia by the action of ribonuclease demonstrates that it represents microscopically visible aggregates of cytoplasmic ribonucleoprotein, presumably of ribosomal origin. Up to 40% of the total circulating erythrocytes may be stippled in foetal and newborn animals. There is then a progressive decline in incidence until adult levels are attained at least by 20 weeks of age. The bone marrow of the adult gerbil contains a higher proportion of stippled red cells than the circulating blood. The stippling can be either coarse or fine and observable in both polychromatophilic and orthochromic cells. It is suggested that erythrocytes with basophilic stippling are relatively immature red cells still demonstrating remnants of cytoplasmic ribonucleoprotein.", "PMID": 979133}
{"id": "pubmed23n0032_12014", "title": "[Method of computing positive pressure in the human lung following space cabin decompression].", "content": "The paper presents a mathematical model and differential equations to be used in computer-aided estimations of the positive pressure in human lugs upon space cabin blast decompression. The paper gives a simplified method of computing the pressure which utilizes a special diagram of decompression of a rigid container communicating with the cabin through a hole of a constant area. The characteristic feature of the method is a simultaneous computation of the air outflowing from the lungs and their expansion during decompression. The paper illustrates the advantage of the simultaneous computation of the processes and specifies the pulmonary regions of positive pressure at given blast decompressions. The paper compares theoretical and experimental (data by foreign researchers) peak values of the positive pressure in human lungs during blast decompression, indicating good agreement of the results.", "contents": "[Method of computing positive pressure in the human lung following space cabin decompression]. The paper presents a mathematical model and differential equations to be used in computer-aided estimations of the positive pressure in human lugs upon space cabin blast decompression. The paper gives a simplified method of computing the pressure which utilizes a special diagram of decompression of a rigid container communicating with the cabin through a hole of a constant area. The characteristic feature of the method is a simultaneous computation of the air outflowing from the lungs and their expansion during decompression. The paper illustrates the advantage of the simultaneous computation of the processes and specifies the pulmonary regions of positive pressure at given blast decompressions. The paper compares theoretical and experimental (data by foreign researchers) peak values of the positive pressure in human lungs during blast decompression, indicating good agreement of the results.", "PMID": 979124}
{"id": "pubmed23n0032_12015", "title": "Retinal detachment in the rat.", "content": "Retinal detachment is uncommon in the rat. Retinal atrophy, rosette formation and folding of the retina are some of the sequelae following reattachment.", "contents": "Retinal detachment in the rat. Retinal detachment is uncommon in the rat. Retinal atrophy, rosette formation and folding of the retina are some of the sequelae following reattachment.", "PMID": 979134}
{"id": "pubmed23n0032_12016", "title": "A technique for intravenous injection in the red-eared turtle (Pseudemys scripta elegans).", "content": "A technique is described for exposure and injection of the jugular vein in the red-eared turtle. Satisfactory anaesthesia and muscular relaxation for this procedure was obtained by intramuscular injection of a mixture of chloral hydrate and sodium pentobarbital at a dose of 2.5 ml/kg bodyweight. Induction time was 30-45 minutes. Time for surgical procedure 30-45 minutes. Recovery time 1-8 hours.", "contents": "A technique for intravenous injection in the red-eared turtle (Pseudemys scripta elegans). A technique is described for exposure and injection of the jugular vein in the red-eared turtle. Satisfactory anaesthesia and muscular relaxation for this procedure was obtained by intramuscular injection of a mixture of chloral hydrate and sodium pentobarbital at a dose of 2.5 ml/kg bodyweight. Induction time was 30-45 minutes. Time for surgical procedure 30-45 minutes. Recovery time 1-8 hours.", "PMID": 979135}
{"id": "pubmed23n0032_12017", "title": "A method for the collection of lymph from the prescapular lymph node of unanaethetized sheep.", "content": "A method for cannulating the efferent duct of the prescapular lymph node of sheep was developed and carried out on over 100 occasions so that lymph could be collected from unanaesthetized sheep under physiological conditions. On average the lymph flowed at 9 ml/h and contained 15 X 10(6) lymphocytes/ml. The surgical procedure and relevant anatomical details are described.", "contents": "A method for the collection of lymph from the prescapular lymph node of unanaethetized sheep. A method for cannulating the efferent duct of the prescapular lymph node of sheep was developed and carried out on over 100 occasions so that lymph could be collected from unanaesthetized sheep under physiological conditions. On average the lymph flowed at 9 ml/h and contained 15 X 10(6) lymphocytes/ml. The surgical procedure and relevant anatomical details are described.", "PMID": 979136}
{"id": "pubmed23n0032_12018", "title": "[Pathomorphologic changes in the organs of rats following chronic inhalation exposure to liquid polyethylsiloxane].", "content": "A chronic 90-day inhalation of polyethylsiloxane fluid at a concentration of 10 and 2 mg/m3 produced local irritation and general toxic effect on rats. Local irritation induced catarrhal-desqueamative tracheitis that was accompanied by a microfocal interstitial process in the lungs. The general toxic effect gave rise to interstitial myocarditis that was followed by focal cardiosclerosis and dystrophic lesions of vascular walls and areas of liver parenchyma. Changes in the thymus-lymph system and adrenal structure suggested prolonged protective tension of the animal body. A chronic 90-day inhalation of polyethylsiloxane at a concentration of 0.2 mg/m3 brought about no pathological lesions in the microstructure of animal organs.", "contents": "[Pathomorphologic changes in the organs of rats following chronic inhalation exposure to liquid polyethylsiloxane]. A chronic 90-day inhalation of polyethylsiloxane fluid at a concentration of 10 and 2 mg/m3 produced local irritation and general toxic effect on rats. Local irritation induced catarrhal-desqueamative tracheitis that was accompanied by a microfocal interstitial process in the lungs. The general toxic effect gave rise to interstitial myocarditis that was followed by focal cardiosclerosis and dystrophic lesions of vascular walls and areas of liver parenchyma. Changes in the thymus-lymph system and adrenal structure suggested prolonged protective tension of the animal body. A chronic 90-day inhalation of polyethylsiloxane at a concentration of 0.2 mg/m3 brought about no pathological lesions in the microstructure of animal organs.", "PMID": 979125}
{"id": "pubmed23n0032_12019", "title": "Effect of bodyweight on the rate of glucose uptake by isolated rat muscles.", "content": "The uptake of glucose by isolated extensor digitorum longus muscles was measured in rats of 78-350 g bodyweight. The rate of uptake per unit weight of muscle fell as the weight of the animal increased. It is concluded that in metabolic studies with isolated rat skelatal muscles, only muscles from weight-matched rats should be compared.", "contents": "Effect of bodyweight on the rate of glucose uptake by isolated rat muscles. The uptake of glucose by isolated extensor digitorum longus muscles was measured in rats of 78-350 g bodyweight. The rate of uptake per unit weight of muscle fell as the weight of the animal increased. It is concluded that in metabolic studies with isolated rat skelatal muscles, only muscles from weight-matched rats should be compared.", "PMID": 979137}
{"id": "pubmed23n0032_12020", "title": "Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony.", "content": "Detailed data from a colony of C57BL mice kept for research into ageing processes are given on weights of 13 organs between the ages of 6 and 30 months; pathological findings and x-ray results from animals aged 6, 24 and 30 months; age-specific tumour incidences in 24 organs in 3-month period up to 36 months of age. Results of previously published age-related studies of reproductive capacity, dental abnormalities and brain changes are summarised.", "contents": "Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony. Detailed data from a colony of C57BL mice kept for research into ageing processes are given on weights of 13 organs between the ages of 6 and 30 months; pathological findings and x-ray results from animals aged 6, 24 and 30 months; age-specific tumour incidences in 24 organs in 3-month period up to 36 months of age. Results of previously published age-related studies of reproductive capacity, dental abnormalities and brain changes are summarised.", "PMID": 979138}
{"id": "pubmed23n0032_12021", "title": "The parasitic ecology of the rodent mite, Myobia musculi. III. Lesions in certain host strains.", "content": "Mice (C57BL/6N) from a commercial production colony with ulcerative skin lesions were shown to have low level infestations with Myobia musculi. Lesions were abolished by exposure to dichlorvos-impregnated plastic strips. Experimental reinfestation with M musculi resulted in prompt exacerbation of lesions in previously healed mice. Exacerbated lesions were again abolished by subsequent dichlorvos treatments. The histopathology of such lesions was shown to be similar to those in cats allergic to Otodectes cynotis; and a morphologic basis for an allergic interpretation of the myobic lesions in the C57BL/6 mice was described. Other evidence supporting an allergic interpretation included the heritability of susceptibility to such lesions in certain strains, the paucity of mites required for extensive lesions, the chronic duration of infestation required prior to lesion development, and the apparent anamnesis to experimental reinfestation. A large commercial hybrid production colony requiring C57BL/6 females had an incidence of ulcerative lesions (in females) of 20% and depressed reproductive indices. Two 48 hour treatments at an interval of 14 days utilizing dichlorvos-impregnated strips (1 square inch per cage) abolished lesions and elevated production indices for a period of 5 months.", "contents": "The parasitic ecology of the rodent mite, Myobia musculi. III. Lesions in certain host strains. Mice (C57BL/6N) from a commercial production colony with ulcerative skin lesions were shown to have low level infestations with Myobia musculi. Lesions were abolished by exposure to dichlorvos-impregnated plastic strips. Experimental reinfestation with M musculi resulted in prompt exacerbation of lesions in previously healed mice. Exacerbated lesions were again abolished by subsequent dichlorvos treatments. The histopathology of such lesions was shown to be similar to those in cats allergic to Otodectes cynotis; and a morphologic basis for an allergic interpretation of the myobic lesions in the C57BL/6 mice was described. Other evidence supporting an allergic interpretation included the heritability of susceptibility to such lesions in certain strains, the paucity of mites required for extensive lesions, the chronic duration of infestation required prior to lesion development, and the apparent anamnesis to experimental reinfestation. A large commercial hybrid production colony requiring C57BL/6 females had an incidence of ulcerative lesions (in females) of 20% and depressed reproductive indices. Two 48 hour treatments at an interval of 14 days utilizing dichlorvos-impregnated strips (1 square inch per cage) abolished lesions and elevated production indices for a period of 5 months.", "PMID": 979139}
{"id": "pubmed23n0032_12022", "title": "Maintenance of axenic mice in open cages in mass air flow.", "content": "The effect of vertical air velocities in mass air flow systems on axenic mice maintained in open cages six inches from cages of non-axenic mice was investigated. The experiment was designed to exclude all but the airborne route of contamination. An airspeed of 10 ffet per minute was sufficient to maintain 15 of 16 animals in the axenic state in four experiments when personnel activity was minimal. During personnel activity, which consisted of changing dirty mouse cages, 26 of 32 axenic mice remained uncontaminated in an airstream of 10 and 26 feet per minute while all mice remained axenic in velocities of 35 and 49 feet per minute.", "contents": "Maintenance of axenic mice in open cages in mass air flow. The effect of vertical air velocities in mass air flow systems on axenic mice maintained in open cages six inches from cages of non-axenic mice was investigated. The experiment was designed to exclude all but the airborne route of contamination. An airspeed of 10 ffet per minute was sufficient to maintain 15 of 16 animals in the axenic state in four experiments when personnel activity was minimal. During personnel activity, which consisted of changing dirty mouse cages, 26 of 32 axenic mice remained uncontaminated in an airstream of 10 and 26 feet per minute while all mice remained axenic in velocities of 35 and 49 feet per minute.", "PMID": 979140}
{"id": "pubmed23n0032_12023", "title": "Cell mediated immunity in the chinchilla (Chinchilla laniger): in vitro lymphocyte transformation.", "content": "The cell mediated immune system of the chinchilla (Chinchilla laniger) was examined using both in vivo and in vitro methods. An in vitro lymphocyte transformation microassay was developed utilizing tritiated thymidine incorporation into spleen cells stimulated by the nonspecific mitogen, phytohemagglutinin-M. Peak stimulation of blastogenesis occurred between 48 and 72 hours of incubation with 5 X 10(6) cells/ml in 12.5 mul phytohemagglutinin-M/ml. In spleen cell cultures from chinchillas sensitized 2 weeks previously with Freund's complete adjuvant plus additional Mycobacterium tuberculosis, incubation of 2.5 X 10(6) or 5 X 10(6) cells/ml with 50 mug/ml of the specific antigen, purified protein derivative, produced marked blastogenesis after 96 hours. The blastogenic response of the chinchilla to this specific antigen was extraordinarily high when compared with transformation responses of canine, bovine and avian peripheral blood lymphocytes utilizing a similar microassay system. The macroscopic in vivo delayed cutaneous hypersensitivity response to an intradermal injection of 10 mug purified protein derivative into chinchillas previously sensitized with Freund's complete adjuvant plus Mycobacterium tuberculosis appeared to be deficient. Grossly, erythema and induration were very slight or absent; however, histologically a slight cutaneous inflammatory response of mixed but predominantly mononuclear leukocytes was observed. The chinchilla's unique ability to display an extraordinary in vitro lymphocyte transformation response and its apparent inability to mount a consistent observable delayed cutaneous hypersensitivity response makes it an excellent model for the further study of the relationship of these phenomena to the total concept of cell mediated immunity.", "contents": "Cell mediated immunity in the chinchilla (Chinchilla laniger): in vitro lymphocyte transformation. The cell mediated immune system of the chinchilla (Chinchilla laniger) was examined using both in vivo and in vitro methods. An in vitro lymphocyte transformation microassay was developed utilizing tritiated thymidine incorporation into spleen cells stimulated by the nonspecific mitogen, phytohemagglutinin-M. Peak stimulation of blastogenesis occurred between 48 and 72 hours of incubation with 5 X 10(6) cells/ml in 12.5 mul phytohemagglutinin-M/ml. In spleen cell cultures from chinchillas sensitized 2 weeks previously with Freund's complete adjuvant plus additional Mycobacterium tuberculosis, incubation of 2.5 X 10(6) or 5 X 10(6) cells/ml with 50 mug/ml of the specific antigen, purified protein derivative, produced marked blastogenesis after 96 hours. The blastogenic response of the chinchilla to this specific antigen was extraordinarily high when compared with transformation responses of canine, bovine and avian peripheral blood lymphocytes utilizing a similar microassay system. The macroscopic in vivo delayed cutaneous hypersensitivity response to an intradermal injection of 10 mug purified protein derivative into chinchillas previously sensitized with Freund's complete adjuvant plus Mycobacterium tuberculosis appeared to be deficient. Grossly, erythema and induration were very slight or absent; however, histologically a slight cutaneous inflammatory response of mixed but predominantly mononuclear leukocytes was observed. The chinchilla's unique ability to display an extraordinary in vitro lymphocyte transformation response and its apparent inability to mount a consistent observable delayed cutaneous hypersensitivity response makes it an excellent model for the further study of the relationship of these phenomena to the total concept of cell mediated immunity.", "PMID": 979141}
{"id": "pubmed23n0032_12024", "title": "A survey of fur mites in domestic rabbits.", "content": "A survey of six commercial rabbit colonies was conducted to determine the prevalence of the mite Cheyletiella parasitvorax. This mite was present in all six colonies, and 43.2% of 220 rabbits examined were infested. Listrophorus gibbus, reported only once previously in domestic rabbits in the United States, was found in four of the six colonies, and in 7.3% of the 220 rabbits examined. Non-parasitic mites were found in 3.2% of the samples. Over 50% of the rabbits examined had inapparent mite infestations.", "contents": "A survey of fur mites in domestic rabbits. A survey of six commercial rabbit colonies was conducted to determine the prevalence of the mite Cheyletiella parasitvorax. This mite was present in all six colonies, and 43.2% of 220 rabbits examined were infested. Listrophorus gibbus, reported only once previously in domestic rabbits in the United States, was found in four of the six colonies, and in 7.3% of the 220 rabbits examined. Non-parasitic mites were found in 3.2% of the samples. Over 50% of the rabbits examined had inapparent mite infestations.", "PMID": 979142}
{"id": "pubmed23n0032_12025", "title": "Studies on the peripheral blood and the in vitro response of peripheral blood lymphocytes to common mitogens in Macaca speciosa.", "content": "In a longitudinal study on 13 adult female stumptailed macaques, Macaca speciosa, peripheral blood samples were analyzed hematologically and the in vitro response of the lymphocytes to the common mitogens concanavalin A, phytohemagglutinin and pokeweed mitogen were investigated. The results showed that the total leukocyte and differential counts in each animal remained quite stable throughout the 3-month experiment. The responses in culture to the mitogens showed that there were differences in the stimulation obtained by each mitogen and that the animals may be placed into high, medium and low groups based on differences in their level of response.", "contents": "Studies on the peripheral blood and the in vitro response of peripheral blood lymphocytes to common mitogens in Macaca speciosa. In a longitudinal study on 13 adult female stumptailed macaques, Macaca speciosa, peripheral blood samples were analyzed hematologically and the in vitro response of the lymphocytes to the common mitogens concanavalin A, phytohemagglutinin and pokeweed mitogen were investigated. The results showed that the total leukocyte and differential counts in each animal remained quite stable throughout the 3-month experiment. The responses in culture to the mitogens showed that there were differences in the stimulation obtained by each mitogen and that the animals may be placed into high, medium and low groups based on differences in their level of response.", "PMID": 979143}
{"id": "pubmed23n0032_12026", "title": "The in vitro response to phytomitogens of marsupial leukocytes.", "content": "Leukocytes from two macropod marsupials, the quokka (Setonix brachyuras) and the tammar (Macropus eugenii) were separated from whole blood by dextran sedimentation and established in culture with either phytohemagglutinin-P, concanavalin A, or pokeweed mitogen. The leukocyte response to each mitogen, as measured by the incorporation of tritiated thymidine into DNA, was similar to that described in other experimental systems. These results suggest that the functional elements of the cellular immune response in marsupials may have evolved along very similar lines to those in the eutherian mammals.", "contents": "The in vitro response to phytomitogens of marsupial leukocytes. Leukocytes from two macropod marsupials, the quokka (Setonix brachyuras) and the tammar (Macropus eugenii) were separated from whole blood by dextran sedimentation and established in culture with either phytohemagglutinin-P, concanavalin A, or pokeweed mitogen. The leukocyte response to each mitogen, as measured by the incorporation of tritiated thymidine into DNA, was similar to that described in other experimental systems. These results suggest that the functional elements of the cellular immune response in marsupials may have evolved along very similar lines to those in the eutherian mammals.", "PMID": 979144}
{"id": "pubmed23n0032_12027", "title": "Apparent acaridal dermatitis in a C57BL/6 Nya mouse colony.", "content": "Dermatitis in a breeding colony of black (C57BL/6 Nya) mice was characterized by intense pruritus leading to self-mutilation and death. The cause appeared to be infestation with the mite, Myobia musculi. Ulceration of the skin resulted in exudation of serum proteins and exposure of the animals' immune defenses to microorganisms and irritants, with subsequent hyperplasia of regional lymph nodes and spleen. Affected mice had an increase in sessile plasma cells, serum immunoglobulin concentration, and percent of circulating granulocytes. The mites were eliminated by treatment with a dichlorvos-ronnel combination in liquid form, which was dispensed onto the bedding with an automatic syringe. The incidence of dermatitis was reduced to zero. Our experience with M musculi in two other strains of mice, both white, indicates that pathogenicity of this mite for mice varies according to the strain, sex, age, and individual differences in sensitivity of the mice as well as the mating ratios employed.", "contents": "Apparent acaridal dermatitis in a C57BL/6 Nya mouse colony. Dermatitis in a breeding colony of black (C57BL/6 Nya) mice was characterized by intense pruritus leading to self-mutilation and death. The cause appeared to be infestation with the mite, Myobia musculi. Ulceration of the skin resulted in exudation of serum proteins and exposure of the animals' immune defenses to microorganisms and irritants, with subsequent hyperplasia of regional lymph nodes and spleen. Affected mice had an increase in sessile plasma cells, serum immunoglobulin concentration, and percent of circulating granulocytes. The mites were eliminated by treatment with a dichlorvos-ronnel combination in liquid form, which was dispensed onto the bedding with an automatic syringe. The incidence of dermatitis was reduced to zero. Our experience with M musculi in two other strains of mice, both white, indicates that pathogenicity of this mite for mice varies according to the strain, sex, age, and individual differences in sensitivity of the mice as well as the mating ratios employed.", "PMID": 979145}
{"id": "pubmed23n0032_12028", "title": "Facial alopecia in the rabbit associated with Cheyletiella parasitivorax.", "content": "A facial dermatitis characterized by alopecia over the frontal area with varying degrees of erythema and scale formation was observed in 46 newly arrived New Zealand White rabbits within a 5-month period. The clinical history, nature of the lesion, and laboratory findings indicated that the mite Cheyletiella parasitivorax was the most likely cause of the dermatitis.", "contents": "Facial alopecia in the rabbit associated with Cheyletiella parasitivorax. A facial dermatitis characterized by alopecia over the frontal area with varying degrees of erythema and scale formation was observed in 46 newly arrived New Zealand White rabbits within a 5-month period. The clinical history, nature of the lesion, and laboratory findings indicated that the mite Cheyletiella parasitivorax was the most likely cause of the dermatitis.", "PMID": 979146}
{"id": "pubmed23n0032_12029", "title": "A comparison of ketamine and the combination ketamine-xylazine for effective surgical anesthesia in the rabbit.", "content": "Ketamine alone and the combination ketamine-xylazine were evaluated as surgical anesthetics in rabbits. It was found that ketamine alone provided inadequate analgesia for ventral abdominal incisions or exposure of the femur. The combination of xylazine with ketamine did provide adequate enalgesia for both surgical procedures.", "contents": "A comparison of ketamine and the combination ketamine-xylazine for effective surgical anesthesia in the rabbit. Ketamine alone and the combination ketamine-xylazine were evaluated as surgical anesthetics in rabbits. It was found that ketamine alone provided inadequate analgesia for ventral abdominal incisions or exposure of the femur. The combination of xylazine with ketamine did provide adequate enalgesia for both surgical procedures.", "PMID": 979147}
{"id": "pubmed23n0032_12030", "title": "A device to protect indwelling catheters in miniature swine.", "content": "A simple, sturdy metal box was designed and utilized to protect indwelling central venous and aortic arch catheters in miniature swine. These catheters were easily maintained for 14 days in unrestrained, free-roaming animals while precise amounts of fluids were infused, serial blood samples were taken, and blood pressure recordings and cardiac output measurements were made.", "contents": "A device to protect indwelling catheters in miniature swine. A simple, sturdy metal box was designed and utilized to protect indwelling central venous and aortic arch catheters in miniature swine. These catheters were easily maintained for 14 days in unrestrained, free-roaming animals while precise amounts of fluids were infused, serial blood samples were taken, and blood pressure recordings and cardiac output measurements were made.", "PMID": 979148}
{"id": "pubmed23n0032_12031", "title": "Dermatophilosis in the marble lizard (Calotes mystaceus).", "content": "Dermatophilus congolensis was isolated from cutaneous hyperkeratotic nodules of two marble lizards. The organism was similar to strains of D congolensis previously characterized as mammalian pathogens. The isolate was experimentally transmitted to other marble lizards and to Spb:(SW)BR mice by subcutaneous inoculation and by topical application after skin scarification.", "contents": "Dermatophilosis in the marble lizard (Calotes mystaceus). Dermatophilus congolensis was isolated from cutaneous hyperkeratotic nodules of two marble lizards. The organism was similar to strains of D congolensis previously characterized as mammalian pathogens. The isolate was experimentally transmitted to other marble lizards and to Spb:(SW)BR mice by subcutaneous inoculation and by topical application after skin scarification.", "PMID": 979149}
{"id": "pubmed23n0032_12032", "title": "Uptake and transport of exogenous proteins by respiratory epithelium.", "content": "The tracer proteins, horseradish peroxidase and ferritin, placed in the trachea of guinea pigs were taken up by epithelial cells and transported to the extracellular space. The interval between the introduction of the tracer proteins into the lumen of the trachea and the morphologic demonstration of the porteins in the extracellular space or within the basal portion of the cells was between 30 and 60 minutes. The proteins were transported in vesicles and no penetration of the epithelial intercellular tight junctions was found. The intercellular tight junctions were made permeable to horseradish peroxidase by anesthetic ether and this permeable epithelium was compared to the vesicle type transport. Transepithelial transport of proteins is a possible mechanism for the introduction of antigenic material into the subepithelial lymphoid tissue and this transport may also be of importance in the late onset type of asthma.", "contents": "Uptake and transport of exogenous proteins by respiratory epithelium. The tracer proteins, horseradish peroxidase and ferritin, placed in the trachea of guinea pigs were taken up by epithelial cells and transported to the extracellular space. The interval between the introduction of the tracer proteins into the lumen of the trachea and the morphologic demonstration of the porteins in the extracellular space or within the basal portion of the cells was between 30 and 60 minutes. The proteins were transported in vesicles and no penetration of the epithelial intercellular tight junctions was found. The intercellular tight junctions were made permeable to horseradish peroxidase by anesthetic ether and this permeable epithelium was compared to the vesicle type transport. Transepithelial transport of proteins is a possible mechanism for the introduction of antigenic material into the subepithelial lymphoid tissue and this transport may also be of importance in the late onset type of asthma.", "PMID": 979161}
{"id": "pubmed23n0032_12033", "title": "Isolation of phagocytic cells from the rat renal glomerulus.", "content": "A method is described for the dissociation of rat glomerular cells in vitro. Isolated endothelial cells were characterized by the persistence of fenestrae. Mesangial cells showed a variety of morphologic appearances; if dissociation was performed after an intravenous injection of ferritin, such cells were identified by the presence of large ferritin-filled vacuoles. Intact epithelial cells were rarely seen, presumably because they did not survive the separation procedure. In culture, a high proportion of cells adhered to glass surfaces, were phagocytic, and had labeled heavily in vivo with ferritin; they were, thus, considered to be mesangial in origin. Receptors for immunoglobulin (Fc) and complement (c3) were also detected in such cells after 24 hours in culture, but not immediately after dissociation. Our findings suggest that a population of isolated glomerular cells, probably comprising mainly mesengial cells, is highly phagocytic and has the potential to develop Fc and C3 receptors. We propose that this technique offers a useful approach for the delineation of glomerular cell properties and functions.", "contents": "Isolation of phagocytic cells from the rat renal glomerulus. A method is described for the dissociation of rat glomerular cells in vitro. Isolated endothelial cells were characterized by the persistence of fenestrae. Mesangial cells showed a variety of morphologic appearances; if dissociation was performed after an intravenous injection of ferritin, such cells were identified by the presence of large ferritin-filled vacuoles. Intact epithelial cells were rarely seen, presumably because they did not survive the separation procedure. In culture, a high proportion of cells adhered to glass surfaces, were phagocytic, and had labeled heavily in vivo with ferritin; they were, thus, considered to be mesangial in origin. Receptors for immunoglobulin (Fc) and complement (c3) were also detected in such cells after 24 hours in culture, but not immediately after dissociation. Our findings suggest that a population of isolated glomerular cells, probably comprising mainly mesengial cells, is highly phagocytic and has the potential to develop Fc and C3 receptors. We propose that this technique offers a useful approach for the delineation of glomerular cell properties and functions.", "PMID": 979162}
{"id": "pubmed23n0032_12034", "title": "Indentations of the glomerular basement membrane in renal diseases. A light and electron microscopic study on ultrathin serial sections.", "content": "Indentations of the glomerular basement membrane were observed by light microscopy in ultrathin Epon-embedded serial sections from the renal biopsies of patients who had membranous glomerulonephritis, minimal change glomerulonephrits, acute or resolving exudative glomerulonephritis and focal glomerulonephrits, interstitial nephritis, amyloidosis, rheumatoid arthritis, or ankylosing spondylitis. In patients with membranous glomerulonephritis, acute or resolving exudative glomerulonephritis, amyloidosis, or rheumatoid arthritis, the occurrence of indentations in the glomerular basement membrane differed significantly from that in controls. The presence of indentations did not correlate with proteinuria, hematuria, leukocyturia, arterial hypertension, or with the nephrotic syndrome or its treatment with steroids. Examination of alternate serial sections by light and be electron microscopy showed that the indentations that were light microscopically visible corresponded to craters on the epithelial surface of the glomerular basement membrane seen in the electron microscope. These craters contained protruding portions of the epithelial cells, extracellular electron-lucent material or electron-dense amorphous or striated membranous material. They were often surrounded by spikelike protrusions of the lamina densa. These indentations might represent solitary remnants of former subepithelial deposits.", "contents": "Indentations of the glomerular basement membrane in renal diseases. A light and electron microscopic study on ultrathin serial sections. Indentations of the glomerular basement membrane were observed by light microscopy in ultrathin Epon-embedded serial sections from the renal biopsies of patients who had membranous glomerulonephritis, minimal change glomerulonephrits, acute or resolving exudative glomerulonephritis and focal glomerulonephrits, interstitial nephritis, amyloidosis, rheumatoid arthritis, or ankylosing spondylitis. In patients with membranous glomerulonephritis, acute or resolving exudative glomerulonephritis, amyloidosis, or rheumatoid arthritis, the occurrence of indentations in the glomerular basement membrane differed significantly from that in controls. The presence of indentations did not correlate with proteinuria, hematuria, leukocyturia, arterial hypertension, or with the nephrotic syndrome or its treatment with steroids. Examination of alternate serial sections by light and be electron microscopy showed that the indentations that were light microscopically visible corresponded to craters on the epithelial surface of the glomerular basement membrane seen in the electron microscope. These craters contained protruding portions of the epithelial cells, extracellular electron-lucent material or electron-dense amorphous or striated membranous material. They were often surrounded by spikelike protrusions of the lamina densa. These indentations might represent solitary remnants of former subepithelial deposits.", "PMID": 979163}
{"id": "pubmed23n0032_12035", "title": "Loss of sulfated proteoglycan from the surface of rabbit platelets during adenosine 5'-diphosphate-induced aggregation.", "content": "Rabbit platelets were labeled in vivo by intravenous injection of 35SO4=60 hours before the animals were exsanguinated. The labeled platelets were washed and resuspended in Tyrode's solution containing 0.35 per cent albumin. The aggregated normally upon the addition of ADP and during aggregation 14.0 +/- 2.7 per cent (mean +/- standard deviation) of the labeled material was lost into the suspending medium. The extent of loss was dependent on ADP concentration and was inhibited by inhibitors of ADP-induced aggregation (AMP, ATP, adenosine, prostaglandin E1, parachloromercuribenzensulfonate and lack of calcium). Since neither release of granule contents nor lysis occurs when rabbit platelets are aggregated by ADP, it was concluded that the labeled material that was lost had been part of the surface coat of the platelets. The labeled material has been identified in other studies as a proteoglycan in which the mucopolysaccharide is principally chondroitin sulfate A. Loss of this material did not make the platelets unresponsive to ADP. The platelets did not reincorporate labeled proteoglycan from plasma. Thrombin caused the release of 50 to 80 per cent of the total platelet-labeled material, presumably from both the granules and the surface. The functional significance of the loss of surface proteoglycan during platelet aggregation is unknown.", "contents": "Loss of sulfated proteoglycan from the surface of rabbit platelets during adenosine 5'-diphosphate-induced aggregation. Rabbit platelets were labeled in vivo by intravenous injection of 35SO4=60 hours before the animals were exsanguinated. The labeled platelets were washed and resuspended in Tyrode's solution containing 0.35 per cent albumin. The aggregated normally upon the addition of ADP and during aggregation 14.0 +/- 2.7 per cent (mean +/- standard deviation) of the labeled material was lost into the suspending medium. The extent of loss was dependent on ADP concentration and was inhibited by inhibitors of ADP-induced aggregation (AMP, ATP, adenosine, prostaglandin E1, parachloromercuribenzensulfonate and lack of calcium). Since neither release of granule contents nor lysis occurs when rabbit platelets are aggregated by ADP, it was concluded that the labeled material that was lost had been part of the surface coat of the platelets. The labeled material has been identified in other studies as a proteoglycan in which the mucopolysaccharide is principally chondroitin sulfate A. Loss of this material did not make the platelets unresponsive to ADP. The platelets did not reincorporate labeled proteoglycan from plasma. Thrombin caused the release of 50 to 80 per cent of the total platelet-labeled material, presumably from both the granules and the surface. The functional significance of the loss of surface proteoglycan during platelet aggregation is unknown.", "PMID": 979164}
{"id": "pubmed23n0032_12036", "title": "A morphologic study of rabbit bronchial lymphoid aggregates and lymphoepithelium.", "content": "Ultrastructural studies of the rabbit bronchial mucosa were performed with scanning and transmission electron microscopy. The former revealed that the bronchus-associated lymphoid aggregates could be distinguished from the surrounding tissue, since they were covered with epithelial cells possessing microvilli but not cilia. These lymphoepithelial cells were irregularly placed and provided crevices of varying dimensions at their junctions with other cells. On transmission electron microscopy, these cells showed scanty cytoplasm, several mitochrondria, few other organelles and only rare phagosomes. Just below the epithelium in the lymphoid aggregates, basophil-like cells were seen regularly and were also found in the lymphoepithelium and adjacent normal bronchila epithelium, here lying between cells above the basement membrane. The possible origin of these cells and their potential relationship to lung defense and hypersensitivity disease are discussed.", "contents": "A morphologic study of rabbit bronchial lymphoid aggregates and lymphoepithelium. Ultrastructural studies of the rabbit bronchial mucosa were performed with scanning and transmission electron microscopy. The former revealed that the bronchus-associated lymphoid aggregates could be distinguished from the surrounding tissue, since they were covered with epithelial cells possessing microvilli but not cilia. These lymphoepithelial cells were irregularly placed and provided crevices of varying dimensions at their junctions with other cells. On transmission electron microscopy, these cells showed scanty cytoplasm, several mitochrondria, few other organelles and only rare phagosomes. Just below the epithelium in the lymphoid aggregates, basophil-like cells were seen regularly and were also found in the lymphoepithelium and adjacent normal bronchila epithelium, here lying between cells above the basement membrane. The possible origin of these cells and their potential relationship to lung defense and hypersensitivity disease are discussed.", "PMID": 979165}
{"id": "pubmed23n0032_12037", "title": "Scanning electron microscopy of hepatic ultrastructure: secondary, backscattered, and transmitted electron imaging.", "content": "Several methods of tissue preparation and different modes of operation of the scanning electron microscope were used to study the ultrastructure of rat liver. Rat livers were perfusion fixed with buffered 2 per cent paraformaldehyde or a mixture of 1.5 per cent paraformaldehyde and 1 per cent glutaraldehyde and processed as follows. Tissue blocks were postfixed in buffered 2 per cent osmium tetroxide followed sequentially by the ligand-mediated osmium binding technique, dehydration and cryofracture in ethanol, and critical point drying. They were then examined without metal coating in the scanning electron microscope operating in the secondary electron and backscattered electron modes. Fifty-micrometer sections were cut with a tissue sectioner, stained with lead citrate, postfixed with osmium, dehydrated, critical point dried, and examined in the secondary electron and back-scattered electron modes. Frozen sections (0.25 to 0.75 mum. thick) were cut by the method of Tokuyasu (Toluyasu KT: J Cell Biol 57:551, 1973) and their scanning transmission electron microscope images were examined either with a scanning transmission electron microscope detector or with a conversion stub using the secondary electron detector. Secondary electron images of the liver prepared by ligand-mediated osmium binding and subsequent cryofracture revealed such intracellular structures as cisternae of the endoplasmic reticulum, lysosomes, mitochondria, lipid droplets, nucleolus and nuclear chromatin, as well as the usual surface morphology, Lipocytes in the perisinusoidal space were readily identified. Backscattered electron images. Unembedded frozen sections had little drying artifact and were virtually free of freezing damage. The scanning transmission electron microscope image revealed those organelles visualized by the secondary electron mode in the ligand-mediated osmium binding-treated tissue.", "contents": "Scanning electron microscopy of hepatic ultrastructure: secondary, backscattered, and transmitted electron imaging. Several methods of tissue preparation and different modes of operation of the scanning electron microscope were used to study the ultrastructure of rat liver. Rat livers were perfusion fixed with buffered 2 per cent paraformaldehyde or a mixture of 1.5 per cent paraformaldehyde and 1 per cent glutaraldehyde and processed as follows. Tissue blocks were postfixed in buffered 2 per cent osmium tetroxide followed sequentially by the ligand-mediated osmium binding technique, dehydration and cryofracture in ethanol, and critical point drying. They were then examined without metal coating in the scanning electron microscope operating in the secondary electron and backscattered electron modes. Fifty-micrometer sections were cut with a tissue sectioner, stained with lead citrate, postfixed with osmium, dehydrated, critical point dried, and examined in the secondary electron and back-scattered electron modes. Frozen sections (0.25 to 0.75 mum. thick) were cut by the method of Tokuyasu (Toluyasu KT: J Cell Biol 57:551, 1973) and their scanning transmission electron microscope images were examined either with a scanning transmission electron microscope detector or with a conversion stub using the secondary electron detector. Secondary electron images of the liver prepared by ligand-mediated osmium binding and subsequent cryofracture revealed such intracellular structures as cisternae of the endoplasmic reticulum, lysosomes, mitochondria, lipid droplets, nucleolus and nuclear chromatin, as well as the usual surface morphology, Lipocytes in the perisinusoidal space were readily identified. Backscattered electron images. Unembedded frozen sections had little drying artifact and were virtually free of freezing damage. The scanning transmission electron microscope image revealed those organelles visualized by the secondary electron mode in the ligand-mediated osmium binding-treated tissue.", "PMID": 979166}
{"id": "pubmed23n0032_12038", "title": "The initial structural lesion in serum-induced demyelination in vitro.", "content": "Myelinated cultures of mouse spinal cord were exposed to sera obtained from rabbits affected by experimental allergic encephalomyelitis following challenge with whole white matter in complete Freund's adjuvant. In the presence of complement, the tissue response begins with an increased birefringence of its myelin sheaths. This is rapidly followed by a gamut of changes leading to demyelination. This study reports that, in the absence of complement, the response is arrested at the stage of increased birefringence. In this way, this early stage of the demyelinating process was available for detailed examination by light and electron microscopy. The brightened myelin sheaths appeared with a few hours of exposure and were seen around all axons and sometimes around cell bodies. This was often accompanied by abrupt breaks in the sheaths and angularly shaped myelin figures. Examination by electron microscope revealed a uniform increase in the myelin period from 11 nm. to 22 nm. The normally double intraperiod line was increased to four electron-dense leaflets, the additional two appearing to be derived from the close apposition of an additional electron-dense layer on the outer surface of the myelin sheath or oligodendrocytic membrane. Oligondendrocytes responsed with a prolific growth of processes whose membranes compacted to form swollen myelin. Neurons, astrocytes, and neuropil showed no changes. In its early stages, at least, the swelling was reversible. It would appear, therefore, that we have isolated the first stage of antiserum-induced demyelination in vitro, a stage which is now available for further study.", "contents": "The initial structural lesion in serum-induced demyelination in vitro. Myelinated cultures of mouse spinal cord were exposed to sera obtained from rabbits affected by experimental allergic encephalomyelitis following challenge with whole white matter in complete Freund's adjuvant. In the presence of complement, the tissue response begins with an increased birefringence of its myelin sheaths. This is rapidly followed by a gamut of changes leading to demyelination. This study reports that, in the absence of complement, the response is arrested at the stage of increased birefringence. In this way, this early stage of the demyelinating process was available for detailed examination by light and electron microscopy. The brightened myelin sheaths appeared with a few hours of exposure and were seen around all axons and sometimes around cell bodies. This was often accompanied by abrupt breaks in the sheaths and angularly shaped myelin figures. Examination by electron microscope revealed a uniform increase in the myelin period from 11 nm. to 22 nm. The normally double intraperiod line was increased to four electron-dense leaflets, the additional two appearing to be derived from the close apposition of an additional electron-dense layer on the outer surface of the myelin sheath or oligodendrocytic membrane. Oligondendrocytes responsed with a prolific growth of processes whose membranes compacted to form swollen myelin. Neurons, astrocytes, and neuropil showed no changes. In its early stages, at least, the swelling was reversible. It would appear, therefore, that we have isolated the first stage of antiserum-induced demyelination in vitro, a stage which is now available for further study.", "PMID": 979167}
{"id": "pubmed23n0032_12039", "title": "Some relationships between punishment, stuttering, and galvanic skin responses.", "content": "The simultaneous effects of response-contingent punishment on stuttering behaviors and the frequency of galvanic skin response (GSR) deflections for 10 subjects were investigated. GSR's and stuttering responses were recorded during base rate, treatment, and extinction conditions. The subjects demonstrated a 50% or greater decrease in stuttering frequency during the treatment condition. Combined data for all subjects indicated that the mean frequency of GSR deflections remained stable or declined across conditions of the study. Analysis of individual data revealed that GSR deflections during treatment as compared with base rate increased for four subjects, remained essentially the same in two subjects, and decreased for four subjects. These results suggest that experimental procedures which result in functional punishment effects on stuttering frequency may not be associated with any predictable pattern on concomitant autonomic arousal.", "contents": "Some relationships between punishment, stuttering, and galvanic skin responses. The simultaneous effects of response-contingent punishment on stuttering behaviors and the frequency of galvanic skin response (GSR) deflections for 10 subjects were investigated. GSR's and stuttering responses were recorded during base rate, treatment, and extinction conditions. The subjects demonstrated a 50% or greater decrease in stuttering frequency during the treatment condition. Combined data for all subjects indicated that the mean frequency of GSR deflections remained stable or declined across conditions of the study. Analysis of individual data revealed that GSR deflections during treatment as compared with base rate increased for four subjects, remained essentially the same in two subjects, and decreased for four subjects. These results suggest that experimental procedures which result in functional punishment effects on stuttering frequency may not be associated with any predictable pattern on concomitant autonomic arousal.", "PMID": 979195}
{"id": "pubmed23n0032_12040", "title": "The influence of duration on the effects of time-out from speaking.", "content": "The influence of the variable of duration on the effects of time-out from speaking was investigated. Forty-five adolescent and adult stutterers participated in a study which examined the relative effects of time-out of one, five, 10, and 30 seconds and no time-out. Response-contingent time-out from speaking of all four durations resulted in significant reductions in stuttering frequency while the no time-out control showed no change. Although longer durations tended to effect greater reductions in stuttering frequency, no reliable differences between time-out durations were found. It was concluded that the punishing effects of the time-out from speaking procedure were predominantly due to its involving contingent interruption of speaking, and that the actual duration of the period of silence was comparatively unimportant. Present findings seems to provide little support for the notion that time-out from speaking primarily represents time-out from positive reinforcement.", "contents": "The influence of duration on the effects of time-out from speaking. The influence of the variable of duration on the effects of time-out from speaking was investigated. Forty-five adolescent and adult stutterers participated in a study which examined the relative effects of time-out of one, five, 10, and 30 seconds and no time-out. Response-contingent time-out from speaking of all four durations resulted in significant reductions in stuttering frequency while the no time-out control showed no change. Although longer durations tended to effect greater reductions in stuttering frequency, no reliable differences between time-out durations were found. It was concluded that the punishing effects of the time-out from speaking procedure were predominantly due to its involving contingent interruption of speaking, and that the actual duration of the period of silence was comparatively unimportant. Present findings seems to provide little support for the notion that time-out from speaking primarily represents time-out from positive reinforcement.", "PMID": 979196}
{"id": "pubmed23n0032_12041", "title": "Damage risk: an evaluation of the effects of exposure to 85 versus 90 dBA of noise.", "content": "The purpose of this study was to compare the damage risk of 85 and 90 dBA of white noise for equivalent full-day exposures. The damage risk of the two noise levels was determined by comparing the temporary threshold shift (TTS) of 12 subjects exposed to either 85 or 90 dBA of white noise for equivalent half- and full-day exposures. TTS was determined by comparing the pre- and postexposure binaural audiograms of each subject at 1, 2, 3, 4, 6, and 8 kHz. It was concluded that the potential damage risk, that is, hazardous effect, of 90 dBA is greater than 85 dBA of noise for equivalent full-day exposures. The statistical difference between the overall effects of equivalent exposures to 85 dBA as compared to 90 dBA of noise could not be traced to any one frequency. The damage risk of a full-day exposure to 85 dBA is equivalent to that of a half-day exposure to 90 dBA of noise. Within the limits of this study, TTSt was as effective as TTS2 for estimating the damage risk of noise exposure.", "contents": "Damage risk: an evaluation of the effects of exposure to 85 versus 90 dBA of noise. The purpose of this study was to compare the damage risk of 85 and 90 dBA of white noise for equivalent full-day exposures. The damage risk of the two noise levels was determined by comparing the temporary threshold shift (TTS) of 12 subjects exposed to either 85 or 90 dBA of white noise for equivalent half- and full-day exposures. TTS was determined by comparing the pre- and postexposure binaural audiograms of each subject at 1, 2, 3, 4, 6, and 8 kHz. It was concluded that the potential damage risk, that is, hazardous effect, of 90 dBA is greater than 85 dBA of noise for equivalent full-day exposures. The statistical difference between the overall effects of equivalent exposures to 85 dBA as compared to 90 dBA of noise could not be traced to any one frequency. The damage risk of a full-day exposure to 85 dBA is equivalent to that of a half-day exposure to 90 dBA of noise. Within the limits of this study, TTSt was as effective as TTS2 for estimating the damage risk of noise exposure.", "PMID": 979197}
{"id": "pubmed23n0032_12042", "title": "An electromyographic study of velopharyngeal function in speech.", "content": "Electromyographic (EMG) recordings were obtained from the levator palatini, superior pharyngeal constrictor, middle pharyngeal constrictor, palatoglossus, and palatopharyngeus muscles of three talkers of American English. Bipolar hooked-wire electrodes were used. Each subject read nonsense words composed of three vowels (/i, a, u/), six stop consonants (/p, b, t, d, k, g/), and two nasal consonants (/m, n/) to form various stop-nasal and nasal-stop contrasts. Multiple repetitions of each utterance type were recorded and subsequently processed by computer. The levator palatini was found to be the primary muscle of velopharyngeal closure for each of the subjects. The palatopharyngeus also showed consistent oralization activity for each of the subjects, although the activity of this muscle was strongly affected by vowel environment. Two subjects showed pharyngeal constrictor muscle activity related to oral articulation, but pharyngeal constrictor activity for the third subject was related to vowel quality. Nasal articulation was accomplished by suppression of oral articulation for each subject. Vowel quality affected the strength of EMG signals for lateral and posterior pharyngeal wall muscles. In those cases where activity was different for the three vowels, activity was greatest for /a/.", "contents": "An electromyographic study of velopharyngeal function in speech. Electromyographic (EMG) recordings were obtained from the levator palatini, superior pharyngeal constrictor, middle pharyngeal constrictor, palatoglossus, and palatopharyngeus muscles of three talkers of American English. Bipolar hooked-wire electrodes were used. Each subject read nonsense words composed of three vowels (/i, a, u/), six stop consonants (/p, b, t, d, k, g/), and two nasal consonants (/m, n/) to form various stop-nasal and nasal-stop contrasts. Multiple repetitions of each utterance type were recorded and subsequently processed by computer. The levator palatini was found to be the primary muscle of velopharyngeal closure for each of the subjects. The palatopharyngeus also showed consistent oralization activity for each of the subjects, although the activity of this muscle was strongly affected by vowel environment. Two subjects showed pharyngeal constrictor muscle activity related to oral articulation, but pharyngeal constrictor activity for the third subject was related to vowel quality. Nasal articulation was accomplished by suppression of oral articulation for each subject. Vowel quality affected the strength of EMG signals for lateral and posterior pharyngeal wall muscles. In those cases where activity was different for the three vowels, activity was greatest for /a/.", "PMID": 979198}
{"id": "pubmed23n0032_12043", "title": "Self- versus investigator-administered presumed fluency reinforcing stimuli.", "content": "The effects of self-administered and investigator-administered presumed fluency reinforcing monetary rewards for perceived fluency increases on the disfluency frequency rate of 17 adult stutterers were studied. Subjects read aloud during 11 one-minute segments under three conditions: one in which no reinforcer was administered at the end of each one-minute segment, a self-administered reinforcement condition, and an investigator-administered reinforcement condition. Although less stuttering was found in the two experimental conditions (with significantly less stuttering occurring in the self-administered fluency reinforcing condition than in the control condition), an increase in stuttering was observed in those reading segments which followed the administration of presumed fluency reinforcing stimuli. These results were interpreted to suggest that although the concept of self-reinforcement may be clinically useful, the operant learning paradigm may be insufficient in providing an adequate description of the events taking place following the administration of fluency contingent reinforcing or punishing stimuli.", "contents": "Self- versus investigator-administered presumed fluency reinforcing stimuli. The effects of self-administered and investigator-administered presumed fluency reinforcing monetary rewards for perceived fluency increases on the disfluency frequency rate of 17 adult stutterers were studied. Subjects read aloud during 11 one-minute segments under three conditions: one in which no reinforcer was administered at the end of each one-minute segment, a self-administered reinforcement condition, and an investigator-administered reinforcement condition. Although less stuttering was found in the two experimental conditions (with significantly less stuttering occurring in the self-administered fluency reinforcing condition than in the control condition), an increase in stuttering was observed in those reading segments which followed the administration of presumed fluency reinforcing stimuli. These results were interpreted to suggest that although the concept of self-reinforcement may be clinically useful, the operant learning paradigm may be insufficient in providing an adequate description of the events taking place following the administration of fluency contingent reinforcing or punishing stimuli.", "PMID": 979199}
{"id": "pubmed23n0032_12044", "title": "A cineradiographic study of articulation in two talkers with temporarily induced oral sensory deprivation.", "content": "Cineradiography was used to study the behavior of the lips, tongue, and mandible in two subjects talking normally and under the influence of trigeminal nerve-block anesthesia. The speech sample consisted of isolated words and sentences. The nerve block was administered to eliminate somesthetic sensation in all of the oral articulators except the jaw. Frame-by-frame measurements of lip protrusion, tongue position, and jaw placement were taken from the film data for selected stops, glides, fricatives, and vowels in the speech sample. Comparison of these measurements from the normal and nerve-block condition revealed the following changes in the nerve-block data: (1) reduction in context-appropriate lip protrusion and loss of precision in lip closure activity, which was more noticeable for the upper than the lower lip; (2) a reduction in the precision of tongue articulations particularly on contacts for lingua-alveolar and lingua-velar consonants, apical retroflexion on glides, and steady state postures for lingua-palatal fricatives and vowels; and (3) noticeable alterations in inferior/superior jaw position which was systematically closer to the maxilla for bilabial consonant closures and often reduced or extended in excursion for vowels and other consonants. The data are discussed in terms of the relative effects of an oral somesthetic feedback deficit on speech production, and also with respect to compensation within the articulatory system under conditions of sensory deprivation.", "contents": "A cineradiographic study of articulation in two talkers with temporarily induced oral sensory deprivation. Cineradiography was used to study the behavior of the lips, tongue, and mandible in two subjects talking normally and under the influence of trigeminal nerve-block anesthesia. The speech sample consisted of isolated words and sentences. The nerve block was administered to eliminate somesthetic sensation in all of the oral articulators except the jaw. Frame-by-frame measurements of lip protrusion, tongue position, and jaw placement were taken from the film data for selected stops, glides, fricatives, and vowels in the speech sample. Comparison of these measurements from the normal and nerve-block condition revealed the following changes in the nerve-block data: (1) reduction in context-appropriate lip protrusion and loss of precision in lip closure activity, which was more noticeable for the upper than the lower lip; (2) a reduction in the precision of tongue articulations particularly on contacts for lingua-alveolar and lingua-velar consonants, apical retroflexion on glides, and steady state postures for lingua-palatal fricatives and vowels; and (3) noticeable alterations in inferior/superior jaw position which was systematically closer to the maxilla for bilabial consonant closures and often reduced or extended in excursion for vowels and other consonants. The data are discussed in terms of the relative effects of an oral somesthetic feedback deficit on speech production, and also with respect to compensation within the articulatory system under conditions of sensory deprivation.", "PMID": 979200}
{"id": "pubmed23n0032_12045", "title": "Traits attributed to stuttering and normally fluent males.", "content": "To determine if a stereotype of the \"typical stutterer\" exists and to identify possible differences in that sterotype due to exposure to stuttering, seven groups of subjects having a wide range of possible exposure to stutterers rated four hypothetical concepts (typical eight-year-old male, typical eight-year-old male stutterer, typical adult male, and typical adult male stutterer) on 25 scales arranged in a semantic differential format. These bipolar scales were derived from words previously judged by speech clinicians as descriptive of stutterers and antonyms of those words. It was concluded that a strong stereotype of a stutterer's personal characteristics exists, that the stereotype is predominantly unfavorable, that the stereotype is essentially unaffected by amount of exposure to actual stutterers, and that the traits attributed to boys and men who stutter are similar. Some implications of the study are discussed.", "contents": "Traits attributed to stuttering and normally fluent males. To determine if a stereotype of the \"typical stutterer\" exists and to identify possible differences in that sterotype due to exposure to stuttering, seven groups of subjects having a wide range of possible exposure to stutterers rated four hypothetical concepts (typical eight-year-old male, typical eight-year-old male stutterer, typical adult male, and typical adult male stutterer) on 25 scales arranged in a semantic differential format. These bipolar scales were derived from words previously judged by speech clinicians as descriptive of stutterers and antonyms of those words. It was concluded that a strong stereotype of a stutterer's personal characteristics exists, that the stereotype is predominantly unfavorable, that the stereotype is essentially unaffected by amount of exposure to actual stutterers, and that the traits attributed to boys and men who stutter are similar. Some implications of the study are discussed.", "PMID": 979201}
{"id": "pubmed23n0032_12046", "title": "Second formant transitions of selected consonant-vowel combinations in the speech of deaf and normal-hearing children.", "content": "Although it is well known that the speech produced by the deaf is generally of low intelligibility, the sources of this low speech intelligibility have generally been ascribed either to aberrant articulation of phonemes or inappropriate prosody. This study was designed to determine to what extent a nonsegmental aspect of speech, formant transitions, may differ in the speech of the deaf and of the normal hearing. The initial second formant transitions of the vowels /i/ and /u/ after labial and alveolar consonants (/b, d, f/) were compared in the speech of six normal-hearing and six hearing-impaired adolescents. In the speech of the hearing-impaired subjects, the second formant transitions may be reduced both in time and in frequency. At its onset, the second formant may be nearer to its eventual target frequency than in the speech of the normal subjects. Since formant transitions are important acoustic cues for the adjacent consonants, reduced F2 transitions may be an important factor in the low intelligibility of the speech of the deaf.", "contents": "Second formant transitions of selected consonant-vowel combinations in the speech of deaf and normal-hearing children. Although it is well known that the speech produced by the deaf is generally of low intelligibility, the sources of this low speech intelligibility have generally been ascribed either to aberrant articulation of phonemes or inappropriate prosody. This study was designed to determine to what extent a nonsegmental aspect of speech, formant transitions, may differ in the speech of the deaf and of the normal hearing. The initial second formant transitions of the vowels /i/ and /u/ after labial and alveolar consonants (/b, d, f/) were compared in the speech of six normal-hearing and six hearing-impaired adolescents. In the speech of the hearing-impaired subjects, the second formant transitions may be reduced both in time and in frequency. At its onset, the second formant may be nearer to its eventual target frequency than in the speech of the normal subjects. Since formant transitions are important acoustic cues for the adjacent consonants, reduced F2 transitions may be an important factor in the low intelligibility of the speech of the deaf.", "PMID": 979202}
{"id": "pubmed23n0032_12047", "title": "The ability of stutterers and nonstutterers to initiate and terminate phonation during production of an isolated vowel.", "content": "This study tested the hypothesis that stutterers have difficulty initiating and terminating phonation independent of the acts of running speech and stuttering. Ten young adult stutterers served as the experimental group. They were matched as a group for age and sex with 10 normal speakers. Subjects from both groups were tested individually. The experimental task required that subjects start and stop phonation as quickly as possible upon hearing each member of a seried of 1000-Hz pure tones appear and then disappear. Subjects' vocalizations were permanently recorded on an optical oscillograph. Results showed that both groups improved (shortened) their voice initiation and termination times from the beginning to the end of the experiment. Typically, however, the stutterers were significantly slower than the control subjects on most of the temporal measures.", "contents": "The ability of stutterers and nonstutterers to initiate and terminate phonation during production of an isolated vowel. This study tested the hypothesis that stutterers have difficulty initiating and terminating phonation independent of the acts of running speech and stuttering. Ten young adult stutterers served as the experimental group. They were matched as a group for age and sex with 10 normal speakers. Subjects from both groups were tested individually. The experimental task required that subjects start and stop phonation as quickly as possible upon hearing each member of a seried of 1000-Hz pure tones appear and then disappear. Subjects' vocalizations were permanently recorded on an optical oscillograph. Results showed that both groups improved (shortened) their voice initiation and termination times from the beginning to the end of the experiment. Typically, however, the stutterers were significantly slower than the control subjects on most of the temporal measures.", "PMID": 979203}
{"id": "pubmed23n0032_12048", "title": "Temporal acuity in listeners with sensorineural hearing loss.", "content": "Temporal acuity, the minimum time interval within which the auditory system can discriminate the order of auditory events, was measured for three listeners with normal hearing and for 10 listeners with sensorineural hearing loss. Eight of the 10 listeners with sensorineural loss showed better temporal acuity for conditions with greater loss. The remaining two listeners showed the opposite effect. The temporal acuity results are not well correlated with either speech discrimination scores or measures of recruitment. The temporal acuity results do appear to be correlated with results observed in studies of temporal integration or brief-tone audiometry. Listeners with sensorineural loss tend to have both poor temporal integration and good temporal acuity. This suggests that the two temporal measures may reflect a single time constant in the auditory system.", "contents": "Temporal acuity in listeners with sensorineural hearing loss. Temporal acuity, the minimum time interval within which the auditory system can discriminate the order of auditory events, was measured for three listeners with normal hearing and for 10 listeners with sensorineural hearing loss. Eight of the 10 listeners with sensorineural loss showed better temporal acuity for conditions with greater loss. The remaining two listeners showed the opposite effect. The temporal acuity results are not well correlated with either speech discrimination scores or measures of recruitment. The temporal acuity results do appear to be correlated with results observed in studies of temporal integration or brief-tone audiometry. Listeners with sensorineural loss tend to have both poor temporal integration and good temporal acuity. This suggests that the two temporal measures may reflect a single time constant in the auditory system.", "PMID": 979204}
{"id": "pubmed23n0032_12049", "title": "An examination of the semantic relations reflected in the language usage of normal and language-disordered children.", "content": "Language samples were obtained from 40 children in order to examine semantic relations reflected in language usage as a function of chronological age (three and five years) and linguistic status (normal and language disordered). Normal-disordered comparisons were made under both matched utterance length and matched age conditions. Results are interpreted as supporting the notion that the disordered-language usage reflected semantic relations consistent with an earlier level of development.", "contents": "An examination of the semantic relations reflected in the language usage of normal and language-disordered children. Language samples were obtained from 40 children in order to examine semantic relations reflected in language usage as a function of chronological age (three and five years) and linguistic status (normal and language disordered). Normal-disordered comparisons were made under both matched utterance length and matched age conditions. Results are interpreted as supporting the notion that the disordered-language usage reflected semantic relations consistent with an earlier level of development.", "PMID": 979205}
{"id": "pubmed23n0032_12050", "title": "Anatomical and neuromuscular maturation of the speech mechanism: evidence from acoustic studies.", "content": "This paper surveys acoustic studies of speech development and discusses the data with respect to the anatomical and neuromuscular maturation of the speech mechanism. The acoustic data on various aspects of speech production indicate that the accuracy of motor control improves with age until adult-like performance is achieved at about 11 or 12 years, somewhat after the age at which speech sound acquisition usually is judged to be complete. Other topics of discussion are (1) problems in the spectrographic analysis of children's speech, (2) formant scale factors that relate children's and adults' data, and (3) identification and diagnosis of developmental disorders through acoustic analyses of speech sounds.", "contents": "Anatomical and neuromuscular maturation of the speech mechanism: evidence from acoustic studies. This paper surveys acoustic studies of speech development and discusses the data with respect to the anatomical and neuromuscular maturation of the speech mechanism. The acoustic data on various aspects of speech production indicate that the accuracy of motor control improves with age until adult-like performance is achieved at about 11 or 12 years, somewhat after the age at which speech sound acquisition usually is judged to be complete. Other topics of discussion are (1) problems in the spectrographic analysis of children's speech, (2) formant scale factors that relate children's and adults' data, and (3) identification and diagnosis of developmental disorders through acoustic analyses of speech sounds.", "PMID": 979206}
{"id": "pubmed23n0032_12051", "title": "Complement structures in the language of deaf students.", "content": "Four hundred and twenty-seven deaf students (age 10 to 19 years) and 60 hearing children (age eight to 10 years) judged the grammaticality of sample sentences which contained infinitival or gerundive complements. Results indicated improvement with increasing age for deaf students. Even the youngest hearing students consistently obtained higher scores than most of the deaf students. Although the function of the complement (as subject or object) did not make a difference in performance, the type of complement did. POSS-ing complements were easier than for-to complements. Verb type also influenced performance.", "contents": "Complement structures in the language of deaf students. Four hundred and twenty-seven deaf students (age 10 to 19 years) and 60 hearing children (age eight to 10 years) judged the grammaticality of sample sentences which contained infinitival or gerundive complements. Results indicated improvement with increasing age for deaf students. Even the youngest hearing students consistently obtained higher scores than most of the deaf students. Although the function of the complement (as subject or object) did not make a difference in performance, the type of complement did. POSS-ing complements were easier than for-to complements. Verb type also influenced performance.", "PMID": 979207}
{"id": "pubmed23n0032_12052", "title": "The effects of stimulability on the articulation of /s/ relative to cluster and word frequency of occurrence.", "content": "This study investigated the effects of stimulability on the articulation of cluster nested /s/ in high- and low-frequency clusters and words. A good stimulability group and a poor stimulability group of /s/-defective seven-year-old children were investigated. Statistical analysis did not reveal significant differences between the articulatory performances of the two groups on words or clusters occurring with high or low frequency. Subjects in the good stimulability group obtained significantly fewer errors on the test stimuli than did subjects in the poor stimulability group. Correlational analyses indicated that stimulability was positively correlated with correct numbers of /s/ productions in both clusters and words occurring with both high and low frequency. Subjects' spontaneous /s/ errors on the Templin-Darley screening test were not found to be correlated with their stimulability scores or their imitative /s/ productions in words and clusters occurring with high or low frequencies.", "contents": "The effects of stimulability on the articulation of /s/ relative to cluster and word frequency of occurrence. This study investigated the effects of stimulability on the articulation of cluster nested /s/ in high- and low-frequency clusters and words. A good stimulability group and a poor stimulability group of /s/-defective seven-year-old children were investigated. Statistical analysis did not reveal significant differences between the articulatory performances of the two groups on words or clusters occurring with high or low frequency. Subjects in the good stimulability group obtained significantly fewer errors on the test stimuli than did subjects in the poor stimulability group. Correlational analyses indicated that stimulability was positively correlated with correct numbers of /s/ productions in both clusters and words occurring with both high and low frequency. Subjects' spontaneous /s/ errors on the Templin-Darley screening test were not found to be correlated with their stimulability scores or their imitative /s/ productions in words and clusters occurring with high or low frequencies.", "PMID": 979208}
{"id": "pubmed23n0032_12053", "title": "The influence of three phonological rules of black English on the discrimination of minimal work pairs.", "content": "Minimal word pairs that are presumed to be perceptually difficult to differentiate when spoken in black English were examined relative to (1) black children's performance in differentiating the meanings of their own word pair productions and those of other blacks and whites and (2) white children's performance in differentiating the meanings of word pairs produced by black children. Perceptual errors were significantly greater for whites listening to word pairs produced by blacks than for blacks listening to themselves, other blacks, or whites. No significant differences were found among blacks listening to themselves, other blacks, and whites. Perceptual errors followed predictable patterns that were influenced by three phonological rules of black English. Also, the data suggest that there are phonemic cues that are imperceptible to non-black-English speakers which allow black-English speakers to differentiate word pairs.", "contents": "The influence of three phonological rules of black English on the discrimination of minimal work pairs. Minimal word pairs that are presumed to be perceptually difficult to differentiate when spoken in black English were examined relative to (1) black children's performance in differentiating the meanings of their own word pair productions and those of other blacks and whites and (2) white children's performance in differentiating the meanings of word pairs produced by black children. Perceptual errors were significantly greater for whites listening to word pairs produced by blacks than for blacks listening to themselves, other blacks, or whites. No significant differences were found among blacks listening to themselves, other blacks, and whites. Perceptual errors followed predictable patterns that were influenced by three phonological rules of black English. Also, the data suggest that there are phonemic cues that are imperceptible to non-black-English speakers which allow black-English speakers to differentiate word pairs.", "PMID": 979209}
{"id": "pubmed23n0032_12054", "title": "Factors contributing to the reduction of stuttering during singing.", "content": "This study was conducted to determine if the reduction of stuttering typically observed during singing is associated with altered vocalization or the familiarity of the melody and lyrics of the song sung by the stutterer, or both. Subjects were eight adult male stutterers. Prior to testing, each of these individuals demonstrated that he knew the melody and lyrics of a well-known song from memory. Subsequently, subjects were asked to read these lyrics aloud and then sing them. Next, subjects had to read aloud and then sing a set of unfamiliar lyrics to the conventional melody of the same song. The stutterers' reading and singing performances were audiotaped. The dependent measures of utterance duration and stuttering frequency were extracted from the tapes. Results showed that subjects' utterance durations were significantly longer during singing than reading. The main effects of singing and familiarity were both associated with significant reductions in stuttering frequency. The greatest decrement in stuttering occurred in the condition where subjects sang the familiar melody and lyrics. These findings were interpreted to mean that changes in vocalization cannot account for all of the decrease in stuttering that occurs during singing. During song, the familiarity of the melody and lyrics being produced may also affect stuttering frequency.", "contents": "Factors contributing to the reduction of stuttering during singing. This study was conducted to determine if the reduction of stuttering typically observed during singing is associated with altered vocalization or the familiarity of the melody and lyrics of the song sung by the stutterer, or both. Subjects were eight adult male stutterers. Prior to testing, each of these individuals demonstrated that he knew the melody and lyrics of a well-known song from memory. Subsequently, subjects were asked to read these lyrics aloud and then sing them. Next, subjects had to read aloud and then sing a set of unfamiliar lyrics to the conventional melody of the same song. The stutterers' reading and singing performances were audiotaped. The dependent measures of utterance duration and stuttering frequency were extracted from the tapes. Results showed that subjects' utterance durations were significantly longer during singing than reading. The main effects of singing and familiarity were both associated with significant reductions in stuttering frequency. The greatest decrement in stuttering occurred in the condition where subjects sang the familiar melody and lyrics. These findings were interpreted to mean that changes in vocalization cannot account for all of the decrease in stuttering that occurs during singing. During song, the familiarity of the melody and lyrics being produced may also affect stuttering frequency.", "PMID": 979210}
{"id": "pubmed23n0032_12055", "title": "Latency of vocalization onset: stutterers versus nonstutterers.", "content": "Eleven stutterers and matched controls were asked to produce as quickly as possible each of 26 different syllables following a visual stimulus. Three trials were given for each syllable. Responses were filtered to remove supraglottally produced sounds, and the time between the visual stimulus and the onset of vocalization was measured by a voice-operated relay and a computer's internal clock. The results suggested that stutterers are slower in initiating vocalization across a wide variety of syllables, and the difference averages about 65 msec. Furthermore, when phonologic conditions delayed voice onset by a comparable amount, the stutterers gained enough time so that no significant differences were observed between the two groups. The results are interpreted as suggesting that auditory dysfunction cannot be a cause for slower vocalization reaction time in stutterers but that either vocal dysfunction or a lack of cerebral dominance may be responsible for these differences.", "contents": "Latency of vocalization onset: stutterers versus nonstutterers. Eleven stutterers and matched controls were asked to produce as quickly as possible each of 26 different syllables following a visual stimulus. Three trials were given for each syllable. Responses were filtered to remove supraglottally produced sounds, and the time between the visual stimulus and the onset of vocalization was measured by a voice-operated relay and a computer's internal clock. The results suggested that stutterers are slower in initiating vocalization across a wide variety of syllables, and the difference averages about 65 msec. Furthermore, when phonologic conditions delayed voice onset by a comparable amount, the stutterers gained enough time so that no significant differences were observed between the two groups. The results are interpreted as suggesting that auditory dysfunction cannot be a cause for slower vocalization reaction time in stutterers but that either vocal dysfunction or a lack of cerebral dominance may be responsible for these differences.", "PMID": 979211}
{"id": "pubmed23n0032_12056", "title": "Elicited imitation of selected features of two american English dialects in Head Start children.", "content": "Three measures were used to check the bidialectal imitative facility of 100 black, white, and Spanish-speaking Head Start children. In general, blacks and Spanish-speaking subjects performed more accurately on black English markers than on Standard English markers and whites, the reverse. When the children did make an error on the feature marker they usually substituted the opposing dialectal marker. Blacks and Spanish-speaking subjects were more apt to be accurate on the total sentence when it was given in black English. Several explanations are offered for group similarities and differences.", "contents": "Elicited imitation of selected features of two american English dialects in Head Start children. Three measures were used to check the bidialectal imitative facility of 100 black, white, and Spanish-speaking Head Start children. In general, blacks and Spanish-speaking subjects performed more accurately on black English markers than on Standard English markers and whites, the reverse. When the children did make an error on the feature marker they usually substituted the opposing dialectal marker. Blacks and Spanish-speaking subjects were more apt to be accurate on the total sentence when it was given in black English. Several explanations are offered for group similarities and differences.", "PMID": 979212}
{"id": "pubmed23n0032_12057", "title": "Stuttering: discoordination of phonation with articulation and respiration.", "content": "Complexity of phonatory and respiratory adjustments was systematically simplified in 30 adult stutterers under three speaking conditions: voiced, whispered, and articulated without phonation. Stuttering was reduced considerably when whispering and was practically eliminated when articulating silently. The possibility that stuttering consistently results from complexity of phonatory coordinations with articulation and respiration was strongly supported. Increased speaking rates under conditions that decreased stuttering seemed to be evidence that efficient rhythmical flow of speech is facilitated by simplification of phonatory and respiratory adjustments.", "contents": "Stuttering: discoordination of phonation with articulation and respiration. Complexity of phonatory and respiratory adjustments was systematically simplified in 30 adult stutterers under three speaking conditions: voiced, whispered, and articulated without phonation. Stuttering was reduced considerably when whispering and was practically eliminated when articulating silently. The possibility that stuttering consistently results from complexity of phonatory coordinations with articulation and respiration was strongly supported. Increased speaking rates under conditions that decreased stuttering seemed to be evidence that efficient rhythmical flow of speech is facilitated by simplification of phonatory and respiratory adjustments.", "PMID": 979213}
{"id": "pubmed23n0032_12058", "title": "The influence of selected masking noises on Lombard and sidetone amplification effects.", "content": "The present experiments were designed to test the notion that the Lombard and sidetone amplification effects are related to the masking ability of a presented background noise. In Experiment 1, eight normal-hearing college students spoke in quiet and while listening to five frequency bands of noise which were equated for intensity but differed in ability to interfere with speech intelligibility. Vocal intensity increased as the frequency band, and thus the masking ability of the noise increased. In Experiment 2, eight additional students spoke in quiet and while listening to four of the frequency bands of noise used in Experiment 1. The noises were equated for loudness rather than intensity. Again, vocal intensity increased as the masking ability of the noise increased. In Experiment 3, 20 additional students spoke while listening to 0 and 20 dB of sidetone amplification mixed with three of the noise bands from Experiment 1. The noises were equated for intensity and mixed with sidetone. The sidetone amplification effect increased as the masking ability of the noise increased. The results of these experiments indicate that interference with speech intelligibility is directly related to elicitation of the Lombard and sidetone amplification effects. The loudness or intensity of a noise has little control over vocal intensity independent of the contribution to masking of speech.", "contents": "The influence of selected masking noises on Lombard and sidetone amplification effects. The present experiments were designed to test the notion that the Lombard and sidetone amplification effects are related to the masking ability of a presented background noise. In Experiment 1, eight normal-hearing college students spoke in quiet and while listening to five frequency bands of noise which were equated for intensity but differed in ability to interfere with speech intelligibility. Vocal intensity increased as the frequency band, and thus the masking ability of the noise increased. In Experiment 2, eight additional students spoke in quiet and while listening to four of the frequency bands of noise used in Experiment 1. The noises were equated for loudness rather than intensity. Again, vocal intensity increased as the masking ability of the noise increased. In Experiment 3, 20 additional students spoke while listening to 0 and 20 dB of sidetone amplification mixed with three of the noise bands from Experiment 1. The noises were equated for intensity and mixed with sidetone. The sidetone amplification effect increased as the masking ability of the noise increased. The results of these experiments indicate that interference with speech intelligibility is directly related to elicitation of the Lombard and sidetone amplification effects. The loudness or intensity of a noise has little control over vocal intensity independent of the contribution to masking of speech.", "PMID": 979214}
{"id": "pubmed23n0032_12059", "title": "Some aspects of the verb system in the language of deaf students.", "content": "Approximately 480 deaf students (age 10 to 19 years) and 60 hearing students (age eight to 10 years) were asked to judge the grammaticality of sentences containing auxiliary verbs, of sentences where the verb had been deleted, and of sentences in which the verb tense was not marked. The results indicated that deaf students have considerable difficulty with the verb system of English. This difficulty was most pronounced in the formation of tense and voice and in agreement in number and tense. A possible ordering for the acquisition of tense did emerge; from earliest to latest it is: simple past, future, present progressive, perfective, and passive. Improvement in grammaticality judgments appeared to come from an increase in recognition that incorrect sentences were ungrammatical.", "contents": "Some aspects of the verb system in the language of deaf students. Approximately 480 deaf students (age 10 to 19 years) and 60 hearing students (age eight to 10 years) were asked to judge the grammaticality of sentences containing auxiliary verbs, of sentences where the verb had been deleted, and of sentences in which the verb tense was not marked. The results indicated that deaf students have considerable difficulty with the verb system of English. This difficulty was most pronounced in the formation of tense and voice and in agreement in number and tense. A possible ordering for the acquisition of tense did emerge; from earliest to latest it is: simple past, future, present progressive, perfective, and passive. Improvement in grammaticality judgments appeared to come from an increase in recognition that incorrect sentences were ungrammatical.", "PMID": 979215}
{"id": "pubmed23n0032_12060", "title": "Performance of adult aphasics on a sentence evaluation and revision task.", "content": "Adult aphasics completed a sentence evaluation and revision task to test some aspects of their linguistic competence. Grammatical and ungrammatical sentences served as the stimuli. Ungrammatical sentences were characterized by violations of syntactic or semantic structure, or both. Aphasics evaluated correctly the grammaticality of most stimulus sentences. Incorrect evaluations were associated with sentences characterized by violations of syntactic structure. Aphasics' revisions of sentences that they judged to be ungrammatical were in the direction of appropriate grammatical structures. Omission of morphological endings was the most frequent sentence revision error. Aphasics' errors on both tasks could be accounted for by performance deficits such as inattentiveness to syntactic detail, auditory perceptual impairments, and inefficient lexical retrieval. These findings lend support to the argument that aphasia is best characterized as a performance rather than competence disruption.", "contents": "Performance of adult aphasics on a sentence evaluation and revision task. Adult aphasics completed a sentence evaluation and revision task to test some aspects of their linguistic competence. Grammatical and ungrammatical sentences served as the stimuli. Ungrammatical sentences were characterized by violations of syntactic or semantic structure, or both. Aphasics evaluated correctly the grammaticality of most stimulus sentences. Incorrect evaluations were associated with sentences characterized by violations of syntactic structure. Aphasics' revisions of sentences that they judged to be ungrammatical were in the direction of appropriate grammatical structures. Omission of morphological endings was the most frequent sentence revision error. Aphasics' errors on both tasks could be accounted for by performance deficits such as inattentiveness to syntactic detail, auditory perceptual impairments, and inefficient lexical retrieval. These findings lend support to the argument that aphasia is best characterized as a performance rather than competence disruption.", "PMID": 979216}
{"id": "pubmed23n0032_12061", "title": "Rapid auditory processing in normal and disordered language development.", "content": "Four-and-one-half- to eight-and-one-half-year-old children with normal language development, normal adults, and dysphasic children were tested for their ability to perceive binary sequences of nonverbal auditory stimuli. Performance was studied in relation to the rate of presentation of stimulus sequence, as measured by the time interval between stimulus elements. Of the normal children studied, only the eight-and-one-half-year-old group responded as well as the adult group to auditory patterns composed of complex tones presented in rapid succession (8-305 msec intervals). Normal children six-and-one-half years and older were able to respond to these same auditory patterns when they were presented more slowly (947-4026 msec intervals). The overall pattern of performance of the dysphasic children was not similar to that of any age group of children with normal language development. The dysphasics' performance was significantly poorer than that of even the four-and-one-half-year-old normal group on rapidly presented auditory sequences, but not significantly different from normal children their own age or adults on the same patterns which were presented more slowly. The interrelation of normal development of rapid auditory processing and normal language development is discussed.", "contents": "Rapid auditory processing in normal and disordered language development. Four-and-one-half- to eight-and-one-half-year-old children with normal language development, normal adults, and dysphasic children were tested for their ability to perceive binary sequences of nonverbal auditory stimuli. Performance was studied in relation to the rate of presentation of stimulus sequence, as measured by the time interval between stimulus elements. Of the normal children studied, only the eight-and-one-half-year-old group responded as well as the adult group to auditory patterns composed of complex tones presented in rapid succession (8-305 msec intervals). Normal children six-and-one-half years and older were able to respond to these same auditory patterns when they were presented more slowly (947-4026 msec intervals). The overall pattern of performance of the dysphasic children was not similar to that of any age group of children with normal language development. The dysphasics' performance was significantly poorer than that of even the four-and-one-half-year-old normal group on rapidly presented auditory sequences, but not significantly different from normal children their own age or adults on the same patterns which were presented more slowly. The interrelation of normal development of rapid auditory processing and normal language development is discussed.", "PMID": 979217}
{"id": "pubmed23n0032_12062", "title": "Lead and lag effects associated with the staggered spondaic word test.", "content": "This study was designed to determine to what extent onset times of overlapping spondaic words were controlled in the development of the Staggered Spondaic Word Test (SSW) (List EC), a measure which is used to detect central auditory dysfunction. The results showed that the SSW (List EC) was not well controlled relative to onset times. Further, the scores of 10 normal-hearing listeners who were administered the SSW were found not to suffer when the onset times were closely matched. The results were related to thn of dichotically presented stimuli.", "contents": "Lead and lag effects associated with the staggered spondaic word test. This study was designed to determine to what extent onset times of overlapping spondaic words were controlled in the development of the Staggered Spondaic Word Test (SSW) (List EC), a measure which is used to detect central auditory dysfunction. The results showed that the SSW (List EC) was not well controlled relative to onset times. Further, the scores of 10 normal-hearing listeners who were administered the SSW were found not to suffer when the onset times were closely matched. The results were related to thn of dichotically presented stimuli.", "PMID": 979218}
{"id": "pubmed23n0032_12063", "title": "The \"heart\" of categorical speech discrimination in young infants.", "content": "Categorical discrimination of place of articulation in the stimuli [dae] and [gae] was investigated in three- to four-month-old infants using a 20/20 cardiac orienting response paradigm. In this procedure infants were presented with 20 tokens of one syllable followed immediately by 20 tokens of a change stimulus. Each infant received a between-category shift, a within-category shift, and a no-shift control condition, with the order of conditions completely counterbalanced across subjects. Discrimination was indexed by an orienting response to the onset of the second 20 stimuli. The data were analyzed for evidence of categorical discrimination both within subjects (each condition pooled across orders of presentation) and between subjects, as is typical of infant-categorical discrimination studies. Both sets of results revealed that infants discriminate the cues for place of articulation categorically. These findings demonstrate the generality of the infant categorical discrimination observed with the nonnutritive operant sucking paradigm and indicate the usefulness of the heart rate paradigm employed in the present study for the collection of within-subject speech discrimination data across a wide-range of subject populations.", "contents": "The \"heart\" of categorical speech discrimination in young infants. Categorical discrimination of place of articulation in the stimuli [dae] and [gae] was investigated in three- to four-month-old infants using a 20/20 cardiac orienting response paradigm. In this procedure infants were presented with 20 tokens of one syllable followed immediately by 20 tokens of a change stimulus. Each infant received a between-category shift, a within-category shift, and a no-shift control condition, with the order of conditions completely counterbalanced across subjects. Discrimination was indexed by an orienting response to the onset of the second 20 stimuli. The data were analyzed for evidence of categorical discrimination both within subjects (each condition pooled across orders of presentation) and between subjects, as is typical of infant-categorical discrimination studies. Both sets of results revealed that infants discriminate the cues for place of articulation categorically. These findings demonstrate the generality of the infant categorical discrimination observed with the nonnutritive operant sucking paradigm and indicate the usefulness of the heart rate paradigm employed in the present study for the collection of within-subject speech discrimination data across a wide-range of subject populations.", "PMID": 979219}
{"id": "pubmed23n0032_12064", "title": "Pretreatment factors associated with the outcome of stuttering therapy.", "content": "Measures of stuttering behavior, personality, and attitudes about speaking were obtained from 20 stutterers at the beginning of treatment. These measures were correlated with three measures of stuttering behavior a year after treatment. Pretreatment attitudes were most highly related to outcome. followed by pretreatment stuttering behavior, and then personality measures. Multiple regression analyses of the pretreatment measures demonstrated that combinations of these variables correlated moderately high (r = 0.79, 0.66, 0.73) with outcome. Prediction equations derived from the multiple regression analyses were used to predict outcomes for an independent group of 18 stutterers. Predicted and actual outcomes were shown to be correlated moderately high (r = 0.75, 0.73, 0.51). Suggestions for clinical use of predictive factors are given. Implications for the design of treatment and understanding the nature of stuttering are discussed.", "contents": "Pretreatment factors associated with the outcome of stuttering therapy. Measures of stuttering behavior, personality, and attitudes about speaking were obtained from 20 stutterers at the beginning of treatment. These measures were correlated with three measures of stuttering behavior a year after treatment. Pretreatment attitudes were most highly related to outcome. followed by pretreatment stuttering behavior, and then personality measures. Multiple regression analyses of the pretreatment measures demonstrated that combinations of these variables correlated moderately high (r = 0.79, 0.66, 0.73) with outcome. Prediction equations derived from the multiple regression analyses were used to predict outcomes for an independent group of 18 stutterers. Predicted and actual outcomes were shown to be correlated moderately high (r = 0.75, 0.73, 0.51). Suggestions for clinical use of predictive factors are given. Implications for the design of treatment and understanding the nature of stuttering are discussed.", "PMID": 979220}
{"id": "pubmed23n0032_12065", "title": "A new technique for measuring velopharyngeal orifice area during sustained vowel production: an application of aerodynamic forced oscillation principles.", "content": "A new technique for measuring velopharyngeal orifice area during sustained vowel production is described. The technique is based on a simple oscillatory flow balancing procedure between two aerodynamic branches, a mechanical model of the human upper airway and the subject's upper airway. The area of the velopharyngeal orifice in the model is adjusted until identical oscillatory flows traverse it and the subject's velopharyngeal orifice in response to a common forced oscillatory pressure. When identical oscillatory flows are observed, the area of the adjusted orifice is taken as a measure of the area of the subject's velopharyngeal orifice.", "contents": "A new technique for measuring velopharyngeal orifice area during sustained vowel production: an application of aerodynamic forced oscillation principles. A new technique for measuring velopharyngeal orifice area during sustained vowel production is described. The technique is based on a simple oscillatory flow balancing procedure between two aerodynamic branches, a mechanical model of the human upper airway and the subject's upper airway. The area of the velopharyngeal orifice in the model is adjusted until identical oscillatory flows traverse it and the subject's velopharyngeal orifice in response to a common forced oscillatory pressure. When identical oscillatory flows are observed, the area of the adjusted orifice is taken as a measure of the area of the subject's velopharyngeal orifice.", "PMID": 979221}
{"id": "pubmed23n0032_12066", "title": "Effects of dorsal and ventral lateral hypothalamic lesions on voluntary alcohol consumption by rats.", "content": "Ventral lateral hypothalamic lesions, made before electrical stimulation, blocked the acquisition of a preference for alcohol by rats; but dorsal lateral hypothalamic lesions did not alter their alcohol consumption. Rats offered alcohol on alternate days, without stimulation, increased their alcohol intake but did not develop a stable preference for alcohol.", "contents": "Effects of dorsal and ventral lateral hypothalamic lesions on voluntary alcohol consumption by rats. Ventral lateral hypothalamic lesions, made before electrical stimulation, blocked the acquisition of a preference for alcohol by rats; but dorsal lateral hypothalamic lesions did not alter their alcohol consumption. Rats offered alcohol on alternate days, without stimulation, increased their alcohol intake but did not develop a stable preference for alcohol.", "PMID": 979271}
{"id": "pubmed23n0032_12067", "title": "Food effects on absorption and metabolism of alcohol.", "content": "The concomitant ingestion of various foods with alcohol resulted in a decreased area under the blood alcohol concentration curve, a lower peak concentration and an increased time to reach peak. Michaelis-Menten kinetics indicated a decreased alcohol metabolism rate after the ingestion of carbohydrates or fats.", "contents": "Food effects on absorption and metabolism of alcohol. The concomitant ingestion of various foods with alcohol resulted in a decreased area under the blood alcohol concentration curve, a lower peak concentration and an increased time to reach peak. Michaelis-Menten kinetics indicated a decreased alcohol metabolism rate after the ingestion of carbohydrates or fats.", "PMID": 979272}
{"id": "pubmed23n0032_12068", "title": "The diagnosis of pathological intoxication.", "content": "Subjects with a history of violent or psychotic behavior while under the influence of alcohol were infused with a 25% alcohol solution. Based on electroencephalograms, blood alcohol concentrations and behavioral observations, the results confirm the probable existence of a discrete syndrome of pathological reaction to alcohol without reference to the amount of alcohol consumed.", "contents": "The diagnosis of pathological intoxication. Subjects with a history of violent or psychotic behavior while under the influence of alcohol were infused with a 25% alcohol solution. Based on electroencephalograms, blood alcohol concentrations and behavioral observations, the results confirm the probable existence of a discrete syndrome of pathological reaction to alcohol without reference to the amount of alcohol consumed.", "PMID": 979273}
{"id": "pubmed23n0032_12069", "title": "Personality characteristics of alcoholics and drug addicts in a merged treatment program.", "content": "Groups of alcoholics and drug addicts in a mixed inpatient treatment facility were compared on personality characteristics by sex, age and type of drug. Sex and drug type showed few significant differences; however, age, regardless of drug or sex, proved to be significant on many personality variables.", "contents": "Personality characteristics of alcoholics and drug addicts in a merged treatment program. Groups of alcoholics and drug addicts in a mixed inpatient treatment facility were compared on personality characteristics by sex, age and type of drug. Sex and drug type showed few significant differences; however, age, regardless of drug or sex, proved to be significant on many personality variables.", "PMID": 979274}
{"id": "pubmed23n0032_12070", "title": "Evaluation of training of alcoholism counselors.", "content": "During a 14-month training program for paraprofessional alcoholism counselors some counselors were markedly more successful than others in keeping patients in treatment and these differences persisted over time.", "contents": "Evaluation of training of alcoholism counselors. During a 14-month training program for paraprofessional alcoholism counselors some counselors were markedly more successful than others in keeping patients in treatment and these differences persisted over time.", "PMID": 979275}
{"id": "pubmed23n0032_12071", "title": "A diagnostic formula for alcoholism.", "content": "Clinical pathologies with unusually high morbidities in alcoholic populations were analyzed to determine their capacity to diagnose alcoholism. On the basis of five systemic variables it was possible to diagnose correctly nearly 75% of alcoholic and matched control subjects.", "contents": "A diagnostic formula for alcoholism. Clinical pathologies with unusually high morbidities in alcoholic populations were analyzed to determine their capacity to diagnose alcoholism. On the basis of five systemic variables it was possible to diagnose correctly nearly 75% of alcoholic and matched control subjects.", "PMID": 979276}
{"id": "pubmed23n0032_12072", "title": "Loss of control, heavy drinking and drinking problems in a longitudinal study.", "content": "The utility of conceptualizing alcoholism as a typically progressive disease process with regular, predictable stages is questioned. \"Loss of control\" was found to be only one variable among others in predicting drinking problems. It was not possible to confirm the importance that some disease conceptions of alcoholism assign to it.", "contents": "Loss of control, heavy drinking and drinking problems in a longitudinal study. The utility of conceptualizing alcoholism as a typically progressive disease process with regular, predictable stages is questioned. \"Loss of control\" was found to be only one variable among others in predicting drinking problems. It was not possible to confirm the importance that some disease conceptions of alcoholism assign to it.", "PMID": 979277}
{"id": "pubmed23n0032_12073", "title": "Sex differences in adolescent alcohol and drug use. A disappearing phenomenon.", "content": "In a study of junior and senior high-school students in two Massachusetts communities, few consistent sex differences were found in patterns of alcohol and drug use.", "contents": "Sex differences in adolescent alcohol and drug use. A disappearing phenomenon. In a study of junior and senior high-school students in two Massachusetts communities, few consistent sex differences were found in patterns of alcohol and drug use.", "PMID": 979278}
{"id": "pubmed23n0032_12074", "title": "Sex and marital status in relation to the characteristics of alcoholics.", "content": "Data on 392 married and unmarried men and women alcoholics suggest that certain social antecedents and drinking patterns represent genuine sex differences and are independent of marital status. However, marital status was related to severity of alcoholism, particularly with respect to personal background characteristics and to psychological functioning at the time of treatment.", "contents": "Sex and marital status in relation to the characteristics of alcoholics. Data on 392 married and unmarried men and women alcoholics suggest that certain social antecedents and drinking patterns represent genuine sex differences and are independent of marital status. However, marital status was related to severity of alcoholism, particularly with respect to personal background characteristics and to psychological functioning at the time of treatment.", "PMID": 979279}
{"id": "pubmed23n0032_12075", "title": "Lack of association of genetic traits with alcoholism; C3, Ss and ABO systems.", "content": "A study of association between markers of the Ss system, the C3 system and the ABO system showed no evidence of any association between alcoholism and specific phenotypes.", "contents": "Lack of association of genetic traits with alcoholism; C3, Ss and ABO systems. A study of association between markers of the Ss system, the C3 system and the ABO system showed no evidence of any association between alcoholism and specific phenotypes.", "PMID": 979280}
{"id": "pubmed23n0032_12076", "title": "Drinking and smoking behavior of hospitalized medical patients.", "content": "In a sample of hospitalized medical patients, problem drinking was found to be associated with heavy smoking.", "contents": "Drinking and smoking behavior of hospitalized medical patients. In a sample of hospitalized medical patients, problem drinking was found to be associated with heavy smoking.", "PMID": 979281}
{"id": "pubmed23n0032_12077", "title": "Pharmacological aversive counterconditioning to alcohol in a private hospital; one-year follow-up.", "content": "Of 261 patients treated for alcoholism with pharmacological aversive counterconditioning, 63% remained abstinent for 1 year following treatment.", "contents": "Pharmacological aversive counterconditioning to alcohol in a private hospital; one-year follow-up. Of 261 patients treated for alcoholism with pharmacological aversive counterconditioning, 63% remained abstinent for 1 year following treatment.", "PMID": 979282}
{"id": "pubmed23n0032_12078", "title": "Social-skills training to teach alcoholics to refuse drinks effectively.", "content": "Two alcoholics who received social-skills training in ways of refusing drinks reported some success in applying their new skills in actual social situations after they left the hospital.", "contents": "Social-skills training to teach alcoholics to refuse drinks effectively. Two alcoholics who received social-skills training in ways of refusing drinks reported some success in applying their new skills in actual social situations after they left the hospital.", "PMID": 979283}
{"id": "pubmed23n0032_12079", "title": "Comment on \"A national study of adolescent drinking behavior, attitudes and correlates\".", "content": "A national study of adolescent drinking is criticized on methodological and conceptual grounds, and it is concluded that its results exaggerate the prevalence of problem drinking among adolescents.", "contents": "Comment on \"A national study of adolescent drinking behavior, attitudes and correlates\". A national study of adolescent drinking is criticized on methodological and conceptual grounds, and it is concluded that its results exaggerate the prevalence of problem drinking among adolescents.", "PMID": 979284}
{"id": "pubmed23n0032_12080", "title": "Opitmal age and results in repair of large ventricular septal defects.", "content": "The hospital mortality rate was 9.1 per cent (three deaths) in 33 patients less than 2 years old undergoing primary repair of single large ventricular septal defects (VSD's) since January 1972, and was not related to age; it was zero in 50 patients older than 2 years (Group A). The mortality rate with multiple VSD's was 36.4 per cent, not related to age. Age at operation, preoperative pulmonary vascular resistance, and pulmonary artery pressure were directly related to mean pulmonary artery pressure 5 or more years later in 74 patients (Group B) operated upon between 1955 and 1968. Over-all probability of \"surgical cure\" for single large VSD (surviving the early and late postoperative period with normal or near-normal pulmonary artery pressure 5 years later) is 92 per cent when pulmonary vascular resistance is 4 units - sq. M. and operation performed at age 27 months, 87 per cent when resistance is 8 and operation done at age 6 months, and 80 per cent when resistance is 12 and operation done at less than 6 months of age. These and other probability curves are examined and compared with natural history to determine optimal age for elective repair for large VSD's.", "contents": "Opitmal age and results in repair of large ventricular septal defects. The hospital mortality rate was 9.1 per cent (three deaths) in 33 patients less than 2 years old undergoing primary repair of single large ventricular septal defects (VSD's) since January 1972, and was not related to age; it was zero in 50 patients older than 2 years (Group A). The mortality rate with multiple VSD's was 36.4 per cent, not related to age. Age at operation, preoperative pulmonary vascular resistance, and pulmonary artery pressure were directly related to mean pulmonary artery pressure 5 or more years later in 74 patients (Group B) operated upon between 1955 and 1968. Over-all probability of \"surgical cure\" for single large VSD (surviving the early and late postoperative period with normal or near-normal pulmonary artery pressure 5 years later) is 92 per cent when pulmonary vascular resistance is 4 units - sq. M. and operation performed at age 27 months, 87 per cent when resistance is 8 and operation done at age 6 months, and 80 per cent when resistance is 12 and operation done at less than 6 months of age. These and other probability curves are examined and compared with natural history to determine optimal age for elective repair for large VSD's.", "PMID": 979309}
{"id": "pubmed23n0032_12081", "title": "Pulmonary neoplasm with solitary cerebral metastasis. Results of combined excision.", "content": "Twenty-two patients underwent excision of a primary pulmonary neoplasm and solitary cerebral metastasis. Six patients had metastatic tumor removed on two occasions and there was one operative death in 28 craniotomies. Seven of 22 patients (32 per cent) survived one year following craniotomy and were free of significant symptoms for one year. Survival for at least 9 months and freedom from significant symptoms was achieved in a total of 12 patients (55 per cent). Relief of severe neurologic symptoms for a minimum of 3 months was achieved in 17 patients (77 per cent). The over-all one-year survival rate was 45 per cent and the average survival period is 14 months with 3 patients still living. The following factors had a favorable bearing on the outcome: Stage 1 lung cancer at pulmonary resection, whole-brain radiation therapy, and a longer interval between pulmonary resection and cerebral metastasis. The experience encourages us to pursue an aggressive surgical approach to pulmonary neoplasm and solitary cerebral metastasis.", "contents": "Pulmonary neoplasm with solitary cerebral metastasis. Results of combined excision. Twenty-two patients underwent excision of a primary pulmonary neoplasm and solitary cerebral metastasis. Six patients had metastatic tumor removed on two occasions and there was one operative death in 28 craniotomies. Seven of 22 patients (32 per cent) survived one year following craniotomy and were free of significant symptoms for one year. Survival for at least 9 months and freedom from significant symptoms was achieved in a total of 12 patients (55 per cent). Relief of severe neurologic symptoms for a minimum of 3 months was achieved in 17 patients (77 per cent). The over-all one-year survival rate was 45 per cent and the average survival period is 14 months with 3 patients still living. The following factors had a favorable bearing on the outcome: Stage 1 lung cancer at pulmonary resection, whole-brain radiation therapy, and a longer interval between pulmonary resection and cerebral metastasis. The experience encourages us to pursue an aggressive surgical approach to pulmonary neoplasm and solitary cerebral metastasis.", "PMID": 979310}
{"id": "pubmed23n0032_12082", "title": "Gallium-67 scanning for carcinoma of the lung.", "content": "Gallium-67 citrate scanning was prospectively evaluated in 55 patients who had lung lesions suspected to be primary carcinoma on chest x-ray films and in whom subsequent histologic diagnosis was obtained. Of 47 patients with histologically proved carcinoma of the lung, 44 (94 per cent) had a positive 67Ga scan. No patient with a positive scan had a benign lesion, so that the positive scan accuracy rate was 100 per cent. All 8 patients with a benign lesion and 3 patients with a malignant lesion had negative scans, for a negative scan accurary rate of 72 per cent for benign lesions. These results give statistical validity for the usefulness of the 67Ga scan in diagnosing carcinoma of the lung (p less than 0.001). Tumor cell type had little effect on the sensitivity of 67Ga scan. The 67Ga scan was equally useful in the evaluation of peripheral and central lesions. There was little difference amount T1, T2, and T3 classified lesions in their ability to take up 67Ga. The 67Ga scan was competitive with mediastinoscopy in assessing mediastinal lymph node metastases and provides a noninvasive method of assessing hilar lymph node metastases. There was a good correlation between the clinical staging of patients with lung cancer based on a chest x-ray film and 67Ga scanning and the staging after surgical treatment based on the histology of the resected specimens.", "contents": "Gallium-67 scanning for carcinoma of the lung. Gallium-67 citrate scanning was prospectively evaluated in 55 patients who had lung lesions suspected to be primary carcinoma on chest x-ray films and in whom subsequent histologic diagnosis was obtained. Of 47 patients with histologically proved carcinoma of the lung, 44 (94 per cent) had a positive 67Ga scan. No patient with a positive scan had a benign lesion, so that the positive scan accuracy rate was 100 per cent. All 8 patients with a benign lesion and 3 patients with a malignant lesion had negative scans, for a negative scan accurary rate of 72 per cent for benign lesions. These results give statistical validity for the usefulness of the 67Ga scan in diagnosing carcinoma of the lung (p less than 0.001). Tumor cell type had little effect on the sensitivity of 67Ga scan. The 67Ga scan was equally useful in the evaluation of peripheral and central lesions. There was little difference amount T1, T2, and T3 classified lesions in their ability to take up 67Ga. The 67Ga scan was competitive with mediastinoscopy in assessing mediastinal lymph node metastases and provides a noninvasive method of assessing hilar lymph node metastases. There was a good correlation between the clinical staging of patients with lung cancer based on a chest x-ray film and 67Ga scanning and the staging after surgical treatment based on the histology of the resected specimens.", "PMID": 979311}
{"id": "pubmed23n0032_12083", "title": "Acute aortic dissection from cross-clamp injury.", "content": "Acute dissection of the ascending aorta secondary to cross-clamp injury can be successfully managed if the problem is recognized immediately. Bypass must be instituted after recannulation at a point distal to the innominate artery so that proper exposure of the site of injury can be obtained. Systemic as well as local hypothermia for myocardial preservation are both necessary. Direct suture closure of all layers at the site of dissection over Teflon felt can terminate this process.", "contents": "Acute aortic dissection from cross-clamp injury. Acute dissection of the ascending aorta secondary to cross-clamp injury can be successfully managed if the problem is recognized immediately. Bypass must be instituted after recannulation at a point distal to the innominate artery so that proper exposure of the site of injury can be obtained. Systemic as well as local hypothermia for myocardial preservation are both necessary. Direct suture closure of all layers at the site of dissection over Teflon felt can terminate this process.", "PMID": 979312}
{"id": "pubmed23n0032_12084", "title": "Traumatic rupture of the aorta. A five-year experience.", "content": "In the five-year period ending in October, 1975, 31 consecutive patients with traumatic rupture of the thoracic aorta underwent surgery at the University of Maryland Hospital or the Maryland Institute for Emergency Medicine. All cases were confirmed by preoperative aortogram. Rupture was confined to one or more sites in the descending thoracic aorta at or distal to the origin of the left subclavian artery. The age was a mean of 26 years. Operation was done within an average of 18 hours after injury. Significant nonthoracic injuries were present in every case. Six patients with positive findings on peritoneal lavage underwent exploratory laparotomy prior to thoracotomy because of shock. Surgical repair was done by use of left heart bypass in 2 cases (one death), a passive aorta-aorta shunt in 23 cases (5 deaths), and without shunt or bypass in 6 cases (no deaths). An end-to-end tubular Dacron graft was used to reconstruct the aorta in all but one patient. Over-all survival rate was 25 of 31 patients (81 per cent). Paraplegia developed in one patient and renal failure in 3 patients (2 deaths) in the aorta-aorta shunt group. Hypertension was present in 18 (72 per cent) of the survivors. Palsy of the left recurrent laryngeal nerve persisted in 8 (32 per cent) of the survivors. Two of the deaths were related to technical problems of the shunting procedure and 2 to intrapleural exsanguination before proximal aortic control could be achieved. Complications and blood loss were reduced in the group with no shunt. The series lends support to the rigorous aortographic search for ruptured thoracic aortas in trauma patients with widened mediastinum. Once experience has been gained with shunting techniques, tears of the descending thoracic aorta may be safely repaired without shunt if done expeditiously.", "contents": "Traumatic rupture of the aorta. A five-year experience. In the five-year period ending in October, 1975, 31 consecutive patients with traumatic rupture of the thoracic aorta underwent surgery at the University of Maryland Hospital or the Maryland Institute for Emergency Medicine. All cases were confirmed by preoperative aortogram. Rupture was confined to one or more sites in the descending thoracic aorta at or distal to the origin of the left subclavian artery. The age was a mean of 26 years. Operation was done within an average of 18 hours after injury. Significant nonthoracic injuries were present in every case. Six patients with positive findings on peritoneal lavage underwent exploratory laparotomy prior to thoracotomy because of shock. Surgical repair was done by use of left heart bypass in 2 cases (one death), a passive aorta-aorta shunt in 23 cases (5 deaths), and without shunt or bypass in 6 cases (no deaths). An end-to-end tubular Dacron graft was used to reconstruct the aorta in all but one patient. Over-all survival rate was 25 of 31 patients (81 per cent). Paraplegia developed in one patient and renal failure in 3 patients (2 deaths) in the aorta-aorta shunt group. Hypertension was present in 18 (72 per cent) of the survivors. Palsy of the left recurrent laryngeal nerve persisted in 8 (32 per cent) of the survivors. Two of the deaths were related to technical problems of the shunting procedure and 2 to intrapleural exsanguination before proximal aortic control could be achieved. Complications and blood loss were reduced in the group with no shunt. The series lends support to the rigorous aortographic search for ruptured thoracic aortas in trauma patients with widened mediastinum. Once experience has been gained with shunting techniques, tears of the descending thoracic aorta may be safely repaired without shunt if done expeditiously.", "PMID": 979313}
{"id": "pubmed23n0032_12085", "title": "The contribution of anticoagulants to platelet dysfunction with extracorporeal circulation.", "content": "This investigations evaluates the effects of anticoagulants on platelet function. Fresh human blood from 40 nonmedicated volunteers was anticoagulated with 4.3 units per milliliter heparin and/or acid-citrate-dextrose (ACD) solution 1:9. Retention of platelets from whole blood on glass beads was performed by the method of Bowie. Platelet retention of heparinized blood averaged 88.1 +/- S.E. 1.5 per cent; ACD platelets averaged 24.6 +/- S.E. 2.8 per cent. Platelet retention with citrate-phosphate-dextrose (CPD) and ethylenediaminetetraacetic acid (EDTA) yielded 26.0 +/- S.E. 3.9 per cent and 19.1 +/- S.E. 7.5 respectively. The addition of ACD to heparinized blood decreased platelet retention (19.7 +/- S.E. 3.1 per cent). The addition of heparin to ACD or CPD blood did not alter the original decreased retention. Calcium added, even in excess, to blood containing heparin and ACD did not reverse the depressed retention (29.3 +/- S.E. 4.6 per cent). The substitution of CPD gave similar results. With mixtures of separately collected ACD and heparininzed blood, depression of platelet retention was directly proportional to the amount of ACD blood present. Altering the pH of the ACD blood did not affect its depressed retention of platelets. Neutralizing heparinized blood 50 per cent with protamine or Polybrene also significantly depressed platelet retention 34.6 +/- S.E. 5.8 per cent and 35.5 +/- S.E. 4.0 per cent, respectively. Neither protamine nor Polybrene had any effect upon ACD blood. These data indicate that anticoagulants may play a significant role in the depressed platelt function observed during and following extracorporeal circulation.", "contents": "The contribution of anticoagulants to platelet dysfunction with extracorporeal circulation. This investigations evaluates the effects of anticoagulants on platelet function. Fresh human blood from 40 nonmedicated volunteers was anticoagulated with 4.3 units per milliliter heparin and/or acid-citrate-dextrose (ACD) solution 1:9. Retention of platelets from whole blood on glass beads was performed by the method of Bowie. Platelet retention of heparinized blood averaged 88.1 +/- S.E. 1.5 per cent; ACD platelets averaged 24.6 +/- S.E. 2.8 per cent. Platelet retention with citrate-phosphate-dextrose (CPD) and ethylenediaminetetraacetic acid (EDTA) yielded 26.0 +/- S.E. 3.9 per cent and 19.1 +/- S.E. 7.5 respectively. The addition of ACD to heparinized blood decreased platelet retention (19.7 +/- S.E. 3.1 per cent). The addition of heparin to ACD or CPD blood did not alter the original decreased retention. Calcium added, even in excess, to blood containing heparin and ACD did not reverse the depressed retention (29.3 +/- S.E. 4.6 per cent). The substitution of CPD gave similar results. With mixtures of separately collected ACD and heparininzed blood, depression of platelet retention was directly proportional to the amount of ACD blood present. Altering the pH of the ACD blood did not affect its depressed retention of platelets. Neutralizing heparinized blood 50 per cent with protamine or Polybrene also significantly depressed platelet retention 34.6 +/- S.E. 5.8 per cent and 35.5 +/- S.E. 4.0 per cent, respectively. Neither protamine nor Polybrene had any effect upon ACD blood. These data indicate that anticoagulants may play a significant role in the depressed platelt function observed during and following extracorporeal circulation.", "PMID": 979314}
{"id": "pubmed23n0032_12086", "title": "Effects of pulsatile and nonpulsatile coronary perfusion on performance of the canine left ventricle.", "content": "This study compares the effect of pulsatile (Group C, Fib/P) and nonpulsatile (Group B, Fib/NP) coronary perfusion on myocardial performance during 2 hours of normothermic ventricular fibrillation. Group A (BH/NP), used as a base-line observation, consisted of 2 hours of nonpulsatile coronary perfusion in beating hearts. The assessment of ventricular performance included diastolic ventricular compliance, myocardial oxygen consumption and lactate extraction, regional myocardial blood flow, and histology. After 120 minutes of ventricular fibrillation, Group C showed normal ventricular diastolic compliance as compared to a 50 per cent decrease in Group B (p less than 0.01). Myocardial oxygen consumption was not significantly different from that in Group B. Because of a 70 per cent increase in oxygen extraction above Group B (p less than 0.05), total left ventricular myocardial blood flow was reduced (103 +/- 23 versus 260 +/- 36 ml. per 100 Gm. per minute, p less than 0.05) and had near-constant resistance. Lactate extraction was significantly greater and more stable as compared to Group B (9.28 +/- 1.33 versus 1.8 +/- 1.08, p less than 0.05). Left ventricular endocardial/epicardial flow ratio was greater in Group C (1.21 +/- 0.08 versus 1.06 +/- 0.06, p less than 0.05). Minimal subendocardial histologic changes were present as compared to the marked patchy subendocardial ischemic changes seen in Group B. The results demonstrate that the addition of pulsatile flow to coronary perfusion minimized the deleterious effects of prolonged ventricular fibrillation on myocardial performance.", "contents": "Effects of pulsatile and nonpulsatile coronary perfusion on performance of the canine left ventricle. This study compares the effect of pulsatile (Group C, Fib/P) and nonpulsatile (Group B, Fib/NP) coronary perfusion on myocardial performance during 2 hours of normothermic ventricular fibrillation. Group A (BH/NP), used as a base-line observation, consisted of 2 hours of nonpulsatile coronary perfusion in beating hearts. The assessment of ventricular performance included diastolic ventricular compliance, myocardial oxygen consumption and lactate extraction, regional myocardial blood flow, and histology. After 120 minutes of ventricular fibrillation, Group C showed normal ventricular diastolic compliance as compared to a 50 per cent decrease in Group B (p less than 0.01). Myocardial oxygen consumption was not significantly different from that in Group B. Because of a 70 per cent increase in oxygen extraction above Group B (p less than 0.05), total left ventricular myocardial blood flow was reduced (103 +/- 23 versus 260 +/- 36 ml. per 100 Gm. per minute, p less than 0.05) and had near-constant resistance. Lactate extraction was significantly greater and more stable as compared to Group B (9.28 +/- 1.33 versus 1.8 +/- 1.08, p less than 0.05). Left ventricular endocardial/epicardial flow ratio was greater in Group C (1.21 +/- 0.08 versus 1.06 +/- 0.06, p less than 0.05). Minimal subendocardial histologic changes were present as compared to the marked patchy subendocardial ischemic changes seen in Group B. The results demonstrate that the addition of pulsatile flow to coronary perfusion minimized the deleterious effects of prolonged ventricular fibrillation on myocardial performance.", "PMID": 979315}
{"id": "pubmed23n0032_12087", "title": "Clinical and hemodynamic criteria for use of the intra-aortic balloon pump in patients requiring cardiac surgery.", "content": "In order to establish criteria for elective use of the intra-aortic balloon pump (IABP) in patients having cardiac surgery, we conducted a retrospective study of 43 patients who required counterpulsation, because of inability to be weaned from cardiopulmonary bypass, between May, 1972, and June, 1974. Patients in cardiogenic shock preoperatively were excluded. The 43 patients included 23 (Group A) who had severe preoperative left ventricular dysfunction with a mean cardiac index less than 1.8 L. per minute per square meter, ejection fraction less than 30 per cent, and end-diastolic pressure greater than 22 mm. Hg; 20 patients (Group B) had a combination of moderate cardiac dysfunction (cardiac index less than 2.2, ejection fraction less than 40, end-diastolic pressure less than 18) in the presence of acute infarction or severe aortic stenosis (gradient greater than 80 mm. Hg) with or without coronary disease. An inverse relationship was noted between survival and delay from completion of operation to the use of 1ABP. Thirty-two of 43 patients were weaned off bypass and were balloon assisted for 12 to 96 hours postoperatively; 25 patients were discharged (58 per cent). In Subgroup A, 14 of 23 (60 per cent) and, in Subgroup B, 9 of 20 (45 per cent) were long-term survivors. Based on these findings, 45 patients were operated upon between June, 1974, and December, 1975, with elective use of 1ABP and were assessed by serial hemodynamic studies. Sixteen had severe preoperative left ventricular dysfunction similar to Subgroup A and 29 had moderate dysfunction in combination with pathology similar to Subgroup B. Fifteen of these patients were hemodynamically unstable at time of arrival in the operating room; 1ABP was inserted under local anesthesia. Thirty-nine patients (87 per cent) were weaned off bypass and were hospital survivors. In Subgroup A, 13 of 16 (81 per cent) and, in Group B, 21 of 29 (72 per cent) were long-term survivors. Criteria for elective use of 1ABP in cardiac surgery should include severe preoperative left ventricular dysfunction or a combination of moderate dysfunction with coronary or valvular pathology. Elective 1ABP improves the survival with trivial iatrogenic morbidity.", "contents": "Clinical and hemodynamic criteria for use of the intra-aortic balloon pump in patients requiring cardiac surgery. In order to establish criteria for elective use of the intra-aortic balloon pump (IABP) in patients having cardiac surgery, we conducted a retrospective study of 43 patients who required counterpulsation, because of inability to be weaned from cardiopulmonary bypass, between May, 1972, and June, 1974. Patients in cardiogenic shock preoperatively were excluded. The 43 patients included 23 (Group A) who had severe preoperative left ventricular dysfunction with a mean cardiac index less than 1.8 L. per minute per square meter, ejection fraction less than 30 per cent, and end-diastolic pressure greater than 22 mm. Hg; 20 patients (Group B) had a combination of moderate cardiac dysfunction (cardiac index less than 2.2, ejection fraction less than 40, end-diastolic pressure less than 18) in the presence of acute infarction or severe aortic stenosis (gradient greater than 80 mm. Hg) with or without coronary disease. An inverse relationship was noted between survival and delay from completion of operation to the use of 1ABP. Thirty-two of 43 patients were weaned off bypass and were balloon assisted for 12 to 96 hours postoperatively; 25 patients were discharged (58 per cent). In Subgroup A, 14 of 23 (60 per cent) and, in Subgroup B, 9 of 20 (45 per cent) were long-term survivors. Based on these findings, 45 patients were operated upon between June, 1974, and December, 1975, with elective use of 1ABP and were assessed by serial hemodynamic studies. Sixteen had severe preoperative left ventricular dysfunction similar to Subgroup A and 29 had moderate dysfunction in combination with pathology similar to Subgroup B. Fifteen of these patients were hemodynamically unstable at time of arrival in the operating room; 1ABP was inserted under local anesthesia. Thirty-nine patients (87 per cent) were weaned off bypass and were hospital survivors. In Subgroup A, 13 of 16 (81 per cent) and, in Group B, 21 of 29 (72 per cent) were long-term survivors. Criteria for elective use of 1ABP in cardiac surgery should include severe preoperative left ventricular dysfunction or a combination of moderate dysfunction with coronary or valvular pathology. Elective 1ABP improves the survival with trivial iatrogenic morbidity.", "PMID": 979316}
{"id": "pubmed23n0032_12088", "title": "Fresh platelet-rich plasma from a flow-through centrifuge for lamb heart perfusion at 13degrees C. Protective effect of added erythrocytes.", "content": "Isolated lamb hearts perfused at 13degrees C. with acellular perfusates developed progressive intersitital edema and a rise in vascular resistance. They did not exhbit any electrical or mechanical activity. In contrast, hearts perfused with whole fresh blood remained well preserved, had no edema or change in vascular resistance, and contracted vigorously while being perfused at 10degrees and 13degrees C. This study was designed to determine which particular component(s) of whole blood contributed to improved cardiac preservation. Isolated lamb hearts were perfused for 18 hours at 13degrees C. with plasma containing platelets and some or no red blood cells. Continuously fresh plasma was obtained from a donor animal by means of a flow-through centrifuge. Hearts perfused at 13degrees C. with fresh plasma of either low or high platelet count contracted during the initial 2 to 4 hours of the perfusion only and were as poorly preserved as hearts perfused with acellular microfiltered plasma. A hematocrit value of 2 to 5 per cent in the plasma perfusate resulted in the hearts being preserved almost as well as with fresh whole blood; they showed a forceful cardiac activity at 13degrees C., there was no edema, the vascular resistance was stable, and after rewarming they had good ventricular function. The improvement in cardiac preservation brought about by addition of a minimal amount of red blood cells suggests a specific effect of erythrocytes on the cardiac microcirculation.", "contents": "Fresh platelet-rich plasma from a flow-through centrifuge for lamb heart perfusion at 13degrees C. Protective effect of added erythrocytes. Isolated lamb hearts perfused at 13degrees C. with acellular perfusates developed progressive intersitital edema and a rise in vascular resistance. They did not exhbit any electrical or mechanical activity. In contrast, hearts perfused with whole fresh blood remained well preserved, had no edema or change in vascular resistance, and contracted vigorously while being perfused at 10degrees and 13degrees C. This study was designed to determine which particular component(s) of whole blood contributed to improved cardiac preservation. Isolated lamb hearts were perfused for 18 hours at 13degrees C. with plasma containing platelets and some or no red blood cells. Continuously fresh plasma was obtained from a donor animal by means of a flow-through centrifuge. Hearts perfused at 13degrees C. with fresh plasma of either low or high platelet count contracted during the initial 2 to 4 hours of the perfusion only and were as poorly preserved as hearts perfused with acellular microfiltered plasma. A hematocrit value of 2 to 5 per cent in the plasma perfusate resulted in the hearts being preserved almost as well as with fresh whole blood; they showed a forceful cardiac activity at 13degrees C., there was no edema, the vascular resistance was stable, and after rewarming they had good ventricular function. The improvement in cardiac preservation brought about by addition of a minimal amount of red blood cells suggests a specific effect of erythrocytes on the cardiac microcirculation.", "PMID": 979317}
{"id": "pubmed23n0032_12089", "title": "Warm ischemic injury of the lung.", "content": "Canine left lower lobes which were subjected to 3 hours of warm ischemia in an atelectatic condition were compared to lobes which had not been stored. During short-term reimplantation studies, the injured lobes were characterized by marked weight gain, diminution in blood flow, increased vascular resistance, and peak airway pressure. The ventilation/perfusion ratios were high and considerable dead-space ventilation was present. Hypoxic vasoconstriction occurred in both control and stored lobes, and shunt determinations did not vary because of the low blood flows observed. Some or all of these physiological changes may serve to develop a simple screening method to evaluate techniques of pulmonary preservation.", "contents": "Warm ischemic injury of the lung. Canine left lower lobes which were subjected to 3 hours of warm ischemia in an atelectatic condition were compared to lobes which had not been stored. During short-term reimplantation studies, the injured lobes were characterized by marked weight gain, diminution in blood flow, increased vascular resistance, and peak airway pressure. The ventilation/perfusion ratios were high and considerable dead-space ventilation was present. Hypoxic vasoconstriction occurred in both control and stored lobes, and shunt determinations did not vary because of the low blood flows observed. Some or all of these physiological changes may serve to develop a simple screening method to evaluate techniques of pulmonary preservation.", "PMID": 979318}
{"id": "pubmed23n0032_12090", "title": "Cardiac manifestations of ochronosis.", "content": "A 68-year-old woman with ochronosis was admitted with congestive heart failure. A typical ejection murmur of aortic stenosis was ausculated and was documented on a phonocardiogram. The patient subsequently died, and at autopsy extensive deposition of ochronotic pigment was found on the aortic valve. This valve had no other anatomic abnormalities. Thus ochronosis should be considered in the differential diagnosis of aortic stenosis.", "contents": "Cardiac manifestations of ochronosis. A 68-year-old woman with ochronosis was admitted with congestive heart failure. A typical ejection murmur of aortic stenosis was ausculated and was documented on a phonocardiogram. The patient subsequently died, and at autopsy extensive deposition of ochronotic pigment was found on the aortic valve. This valve had no other anatomic abnormalities. Thus ochronosis should be considered in the differential diagnosis of aortic stenosis.", "PMID": 979319}
{"id": "pubmed23n0032_12091", "title": "An alternative approach to the closure of the Blalock-Taussig anastomosis at open-heart surgery.", "content": "Dissection for closure of a Blalock-Taussig shunt can occasionally be difficult or hazardous at the time of corrective operation. The technique traditionally used for closure of a Potts anastomosis, in which profound hypothermia and circulatory arrest are used, has been applied successfully in 11 operations for control of 12 Blalock-Taussig anastomoses. The technique is described.", "contents": "An alternative approach to the closure of the Blalock-Taussig anastomosis at open-heart surgery. Dissection for closure of a Blalock-Taussig shunt can occasionally be difficult or hazardous at the time of corrective operation. The technique traditionally used for closure of a Potts anastomosis, in which profound hypothermia and circulatory arrest are used, has been applied successfully in 11 operations for control of 12 Blalock-Taussig anastomoses. The technique is described.", "PMID": 979320}
{"id": "pubmed23n0032_12092", "title": "Pulmonary blastoma.", "content": "Two cases of pulmonary blastoma are reported. One occurred in a 29-year-old man 31 months after a cyst had been excised from the same pericardial-pleural area. The cyst appeared to be of mesothelial origin and, although supported by a cellular stroma, was originally considered to be benign. The morphologic features, location, and possible relationship to a mesothelial lesion suggest an embryonal-mesothelial origin of the tumor. The patient died of recurrent and metastatic disease 9 years after the first resection. The second case is that of a now 74-year-old woman who, over a period of 19 years, has had six separate small peripheral lung tumors excised. They originally resembled fibrous hamartomas but included embryonal-type immature areas and became increasingly more cellular and sarcomatous. The fine structure of the recurrent tumors is that of primitive stroma with few fibrils but no other differentiating features.", "contents": "Pulmonary blastoma. Two cases of pulmonary blastoma are reported. One occurred in a 29-year-old man 31 months after a cyst had been excised from the same pericardial-pleural area. The cyst appeared to be of mesothelial origin and, although supported by a cellular stroma, was originally considered to be benign. The morphologic features, location, and possible relationship to a mesothelial lesion suggest an embryonal-mesothelial origin of the tumor. The patient died of recurrent and metastatic disease 9 years after the first resection. The second case is that of a now 74-year-old woman who, over a period of 19 years, has had six separate small peripheral lung tumors excised. They originally resembled fibrous hamartomas but included embryonal-type immature areas and became increasingly more cellular and sarcomatous. The fine structure of the recurrent tumors is that of primitive stroma with few fibrils but no other differentiating features.", "PMID": 979321}
{"id": "pubmed23n0032_12093", "title": "Infantile achalasia. Brief clinical report.", "content": "Achalasia of the esophagus is an unusual lesion in childhood and is quite rare under the age of one year. The case of a 3-month-old child with esophageal achalasia treated with a Heller esophagomyotomy is illustrated. Seven previously documented cases of achalasia in children under the age of one year are reviewed and the difficulties of diagnosing this lesion in childhood are discussed.", "contents": "Infantile achalasia. Brief clinical report. Achalasia of the esophagus is an unusual lesion in childhood and is quite rare under the age of one year. The case of a 3-month-old child with esophageal achalasia treated with a Heller esophagomyotomy is illustrated. Seven previously documented cases of achalasia in children under the age of one year are reviewed and the difficulties of diagnosing this lesion in childhood are discussed.", "PMID": 979322}
{"id": "pubmed23n0032_12094", "title": "[Complications following vaginal hysterectomy].", "content": "The author analyses complications following 817 vaginal hysterectomies (VH). The mortality rate was 0.24%. Laparotomy during VH was performed in 3 patients (0.36%): twice the commenced VH was completed abdominally (once owing to the overlooked fixed uterus in a very obese patient and once owing to the previously performed Doleris operation). These are so-called unsuccessful vaginal hysterectomies. In the third patient laparotomy was applied owing to the bleeding from the retracted, cut uterine artery. Injuries of the urinary pelvis during the VH procedure occured in two patients: once during the opening of the plica vesico-urinaris and once during the opening of the Douglas sac (in the patient with a previous Doleris operation). Both injuries were cured vaginally. Early complication were recorded in 34.02% (278 cases). Most infections occurred in the small pelvis. Late complications were encountered in 41.07% (207 cases) out of 504 followed-up patients. Granulation tissue has proved to be the most frequent complication in this group of women (33.13%). Incontinentia urinae was found in 2.5% (13 cases).", "contents": "[Complications following vaginal hysterectomy]. The author analyses complications following 817 vaginal hysterectomies (VH). The mortality rate was 0.24%. Laparotomy during VH was performed in 3 patients (0.36%): twice the commenced VH was completed abdominally (once owing to the overlooked fixed uterus in a very obese patient and once owing to the previously performed Doleris operation). These are so-called unsuccessful vaginal hysterectomies. In the third patient laparotomy was applied owing to the bleeding from the retracted, cut uterine artery. Injuries of the urinary pelvis during the VH procedure occured in two patients: once during the opening of the plica vesico-urinaris and once during the opening of the Douglas sac (in the patient with a previous Doleris operation). Both injuries were cured vaginally. Early complication were recorded in 34.02% (278 cases). Most infections occurred in the small pelvis. Late complications were encountered in 41.07% (207 cases) out of 504 followed-up patients. Granulation tissue has proved to be the most frequent complication in this group of women (33.13%). Incontinentia urinae was found in 2.5% (13 cases).", "PMID": 979327}
{"id": "pubmed23n0032_12095", "title": "[Sarcomatous alteration in leiomyoma].", "content": "A patient with a histologically verified leiomyosarcoma is presented. Hysterectomy with bilateral adnexectomy was performed in 1973. Then radiation therapy was applied. Now, five years after the beginning of the therapy, the patient is in a very good condition.", "contents": "[Sarcomatous alteration in leiomyoma]. A patient with a histologically verified leiomyosarcoma is presented. Hysterectomy with bilateral adnexectomy was performed in 1973. Then radiation therapy was applied. Now, five years after the beginning of the therapy, the patient is in a very good condition.", "PMID": 979330}
{"id": "pubmed23n0032_12096", "title": "[Dysgerminoma ovarii].", "content": "The authors present a case of a unilateral, encapsuled, and distinctly defined ovarian dysgerminoma treated by a radical removal of the affected ovary. There were remote isolated metastases in the supraclavicular lymph node one year after the operation. No relapses or other metastases have as yet observed.", "contents": "[Dysgerminoma ovarii]. The authors present a case of a unilateral, encapsuled, and distinctly defined ovarian dysgerminoma treated by a radical removal of the affected ovary. There were remote isolated metastases in the supraclavicular lymph node one year after the operation. No relapses or other metastases have as yet observed.", "PMID": 979331}
{"id": "pubmed23n0032_12097", "title": "[Torsion of the pregnant Fallopian tube with simultaneous intrauterine pregnancy].", "content": "The author describes a rare case of the torsion of the pregnant fallopian tube with the macerated fetus and simultaneous intrauterine pregnancy. He points out dignostic difficulties in such cases.", "contents": "[Torsion of the pregnant Fallopian tube with simultaneous intrauterine pregnancy]. The author describes a rare case of the torsion of the pregnant fallopian tube with the macerated fetus and simultaneous intrauterine pregnancy. He points out dignostic difficulties in such cases.", "PMID": 979332}
{"id": "pubmed23n0032_12098", "title": "[Prolonged and postmature pregnancy terminated in non-hospital institutions].", "content": "Out of 4023 deliveries, the authors came across 291 prolonged ones, of which 39 were postmature deliveries. They followed the issue of these pregnancies in the conditions of non-hospital health care. The mortality rate of postmature deliveries was 10.2%. It is recommended that all prolonged and postmature pregnancies are referred to larger hospital-type maternity wards where more help can be offered to the pregnant woman and where the actual state of the fetoplacental unit can be properly followed. However, in cases when prolonged and postmature pregnancies are to be terminated in a non-hospital institution because of the women's refusal to go a larger maternity ward, it is proposed in present conditions to use as a diagnositc means also amnioscopy in addition to cytohormonal analysis.", "contents": "[Prolonged and postmature pregnancy terminated in non-hospital institutions]. Out of 4023 deliveries, the authors came across 291 prolonged ones, of which 39 were postmature deliveries. They followed the issue of these pregnancies in the conditions of non-hospital health care. The mortality rate of postmature deliveries was 10.2%. It is recommended that all prolonged and postmature pregnancies are referred to larger hospital-type maternity wards where more help can be offered to the pregnant woman and where the actual state of the fetoplacental unit can be properly followed. However, in cases when prolonged and postmature pregnancies are to be terminated in a non-hospital institution because of the women's refusal to go a larger maternity ward, it is proposed in present conditions to use as a diagnositc means also amnioscopy in addition to cytohormonal analysis.", "PMID": 979333}
{"id": "pubmed23n0032_12099", "title": "Juvenilna Metroragija.", "content": "Following the definition and etiology, cases of juvenile bleeding in 66 patients were analysed in connection with the time of its occurrence, its clinical picture and therapy. In the authors' own material the percentage of juvenile bleeding was 4.18. It was noted that juvenile bleeding occurred most frequently in virgins (95.45%), that is in the first three gynecological years (78.79%). The mean value of menarche in juvenile bleeding is 12.7 years. The values of Hb at examination, that is on admission to hospital, illustrate the degree of anemia. There were 38 hospitalized patients, and in 28 patients blod transfusion was applied. The termination of bleeding was achieved by hormone therapy. A successful combination of estrogen-gestagen therapy in the sense of substitution was applied. Only in one case was exploratory curettage performed and this vas due to suspicion of residue following abortion.", "contents": "Juvenilna Metroragija. Following the definition and etiology, cases of juvenile bleeding in 66 patients were analysed in connection with the time of its occurrence, its clinical picture and therapy. In the authors' own material the percentage of juvenile bleeding was 4.18. It was noted that juvenile bleeding occurred most frequently in virgins (95.45%), that is in the first three gynecological years (78.79%). The mean value of menarche in juvenile bleeding is 12.7 years. The values of Hb at examination, that is on admission to hospital, illustrate the degree of anemia. There were 38 hospitalized patients, and in 28 patients blod transfusion was applied. The termination of bleeding was achieved by hormone therapy. A successful combination of estrogen-gestagen therapy in the sense of substitution was applied. Only in one case was exploratory curettage performed and this vas due to suspicion of residue following abortion.", "PMID": 979328}
{"id": "pubmed23n0032_12100", "title": "[2 intrauterine pregnancies after 2 tubal pregnancies].", "content": "A woman with two intrauterine pregnancies after two ectopic tubal pregnancies is presented. The first operation removed the left Fallopian tube (on 4 November 1970), while during the recurrent ectopic pregnancy, plastic surgery was applied in the remaining right tube (24 October 1972) with the enucleation of the fertilized egg after Prochownik's method. The patient became pregnant 3 months after the operation and had spontaneous abortion, with a fetus two-and-a half months old; the second time she became pregnant 17 months after the operation and gave birth to a female live-born, 26 days before the term, weighing 2,900 g and 49 cm high. The authors conclude that best results are obtained when the plastic surgery made in the fallopian tube is as small as possible and when postoperative instillation of the tubes is applied.", "contents": "[2 intrauterine pregnancies after 2 tubal pregnancies]. A woman with two intrauterine pregnancies after two ectopic tubal pregnancies is presented. The first operation removed the left Fallopian tube (on 4 November 1970), while during the recurrent ectopic pregnancy, plastic surgery was applied in the remaining right tube (24 October 1972) with the enucleation of the fertilized egg after Prochownik's method. The patient became pregnant 3 months after the operation and had spontaneous abortion, with a fetus two-and-a half months old; the second time she became pregnant 17 months after the operation and gave birth to a female live-born, 26 days before the term, weighing 2,900 g and 49 cm high. The authors conclude that best results are obtained when the plastic surgery made in the fallopian tube is as small as possible and when postoperative instillation of the tubes is applied.", "PMID": 979334}
{"id": "pubmed23n0032_12101", "title": "[Adrenogenital syndrome in an adolescent].", "content": "A case is presented of primary amenorrhea resulting from the hyperfunction of the adrenal cortex. The condition developed in prepuberty. The mode of the establishment of the diagnosis and the effect of dexamethason treatment are discussed.", "contents": "[Adrenogenital syndrome in an adolescent]. A case is presented of primary amenorrhea resulting from the hyperfunction of the adrenal cortex. The condition developed in prepuberty. The mode of the establishment of the diagnosis and the effect of dexamethason treatment are discussed.", "PMID": 979329}
{"id": "pubmed23n0032_12102", "title": "Impaired myocardial contraction in the chronic stage.", "content": "The consequences of ischemia for the affected myocardium are highly variable. As a result, in chronic coronary heart disease there is little correlation between coronary arterial obstruction and myocardial dysfunction. Dysfunction can be permanent and related to replacement fibrosis or fixed disruption of the chain of events leading to effective contraction. Dysfunction can also be transient, as a direct consequence of acute myocardial ischemia. The mechanical lesions include generalized and localized myocardial dysfunction, mitral incompetence, and rarely, a left-to-right shunt through loss of integrity of the interventricular septum. Diagnosis per se is not difficult, but deciding on the true contribution of any given lesion to the overall symptoms and disability of the patient is. Therapy must be individually planned according to the total set of factors in each patient.", "contents": "Impaired myocardial contraction in the chronic stage. The consequences of ischemia for the affected myocardium are highly variable. As a result, in chronic coronary heart disease there is little correlation between coronary arterial obstruction and myocardial dysfunction. Dysfunction can be permanent and related to replacement fibrosis or fixed disruption of the chain of events leading to effective contraction. Dysfunction can also be transient, as a direct consequence of acute myocardial ischemia. The mechanical lesions include generalized and localized myocardial dysfunction, mitral incompetence, and rarely, a left-to-right shunt through loss of integrity of the interventricular septum. Diagnosis per se is not difficult, but deciding on the true contribution of any given lesion to the overall symptoms and disability of the patient is. Therapy must be individually planned according to the total set of factors in each patient.", "PMID": 979342}
{"id": "pubmed23n0032_12103", "title": "Natural history of coronary heart disease.", "content": "We now possess enough data concerning prognosis so that we can highlight the areas of concentration for the practicing physician. A history of congestive failure, hypertension,or diabetes is of greatest importance. Smoking is in a similar category, but cholesterol elevation is not. Electrocardiographic findings can be used as an immediate discriminator, depending on whether they are normal or abnormal. Further refinements are possible, depending on whether there are ST-segment depressions or elevations, ventricular conduction defects, repetitive ventricular dysrhythmias, left ventricular hypertrophy, or Q waves of prior infarctions. The exercise electrocardiogram provides additional important information and, if markedly abnormal, can detect with reasonable degree of accuracy the presence and degree of ischemic heart disease. The coronary arteriogram, which influences many of the preceding clinical criteria, permits an accurate prediction of five-year mortality and in a preliminary fashion can be integrated with electrocardiographic and ventriculographic abnormalities to derive a significant measure of prognosis. Finally, cardiac function, if assessed according to specific criteria, becomes an extremely important variable in predicting natural history in coronary heart disease.", "contents": "Natural history of coronary heart disease. We now possess enough data concerning prognosis so that we can highlight the areas of concentration for the practicing physician. A history of congestive failure, hypertension,or diabetes is of greatest importance. Smoking is in a similar category, but cholesterol elevation is not. Electrocardiographic findings can be used as an immediate discriminator, depending on whether they are normal or abnormal. Further refinements are possible, depending on whether there are ST-segment depressions or elevations, ventricular conduction defects, repetitive ventricular dysrhythmias, left ventricular hypertrophy, or Q waves of prior infarctions. The exercise electrocardiogram provides additional important information and, if markedly abnormal, can detect with reasonable degree of accuracy the presence and degree of ischemic heart disease. The coronary arteriogram, which influences many of the preceding clinical criteria, permits an accurate prediction of five-year mortality and in a preliminary fashion can be integrated with electrocardiographic and ventriculographic abnormalities to derive a significant measure of prognosis. Finally, cardiac function, if assessed according to specific criteria, becomes an extremely important variable in predicting natural history in coronary heart disease.", "PMID": 979344}
{"id": "pubmed23n0032_12104", "title": "Polydepsipeptides. 5. Experimental conformational analysis of poly(L-alanyl-L-lactic acid) and related model compounds.", "content": "In this paper we report an experimental conformational analysis of the depsipeptide model compounds acetyl-L-alanine methyl ester, acetyl-L-lactic acid N-methylamide, and acetyl-L-alanyl-L-lactic acid N-methylamide and of the sequential polydepsipeptide poly(L-alanyl-L-lactic acid). The model depsipeptides were examined in dilute organic solutions by infrared and nuclear magnetic resonance spectroscopy. Neither acetyl-L-alanine methyl ester nor acetyl-L-lactic acid N-methylamide assumes an intramolecularly hydrogen-bonded conformation. Acetyl-L-alanyl-L-lactic acid N-methylamide, on the other hand, in dilute chloroform or dilute carbon tetrachloride solutions, strongly favors a conformation with an intramolecular hydrogen bond between the N-H hydrogen atom of its N-methylamide group and the carbonyl oxygen atom of its acetyl group. Comparison of theoretical and experimental circular dichroism suggests that poly(L-alanyl-L-lactic acid) is partially ordered in chloroform solution with approximately 50% of its repeat units in the R10 helix, an ordered conformation found by our previous theoretical analysis to have a low intramolecular conformational energy.", "contents": "Polydepsipeptides. 5. Experimental conformational analysis of poly(L-alanyl-L-lactic acid) and related model compounds. In this paper we report an experimental conformational analysis of the depsipeptide model compounds acetyl-L-alanine methyl ester, acetyl-L-lactic acid N-methylamide, and acetyl-L-alanyl-L-lactic acid N-methylamide and of the sequential polydepsipeptide poly(L-alanyl-L-lactic acid). The model depsipeptides were examined in dilute organic solutions by infrared and nuclear magnetic resonance spectroscopy. Neither acetyl-L-alanine methyl ester nor acetyl-L-lactic acid N-methylamide assumes an intramolecularly hydrogen-bonded conformation. Acetyl-L-alanyl-L-lactic acid N-methylamide, on the other hand, in dilute chloroform or dilute carbon tetrachloride solutions, strongly favors a conformation with an intramolecular hydrogen bond between the N-H hydrogen atom of its N-methylamide group and the carbonyl oxygen atom of its acetyl group. Comparison of theoretical and experimental circular dichroism suggests that poly(L-alanyl-L-lactic acid) is partially ordered in chloroform solution with approximately 50% of its repeat units in the R10 helix, an ordered conformation found by our previous theoretical analysis to have a low intramolecular conformational energy.", "PMID": 979338}
{"id": "pubmed23n0032_12105", "title": "Statistical mechanical treatment of protein conformation. 4. A four-state model for specific-sequence copolymers of amino acids.", "content": "One-dimensional short-range interaction models for specific-sequence copolymers of amino acids are being developed in this series of papers. In this paper, our earlier three-state model [involving helical (h), extended (epsilon), and coil (or other) (c) states] is extended to a four-state model by preserving the h and epsilon states, introducing the chain-reversal state (R and S), and redefining the c state. This model involves six parameters (wh, vh, vepsilon, vR, vS, and uc) and requires a 6 X 6 statistical weight matrix. A nearest-neighbor approximation of the four-state model is also formulated; it requires a 5 X 5 matrix, involving the same six parameters. By expressing the statistical weights relative to that of the epsilon state, only five parameters (wh, vh, vR, vS, and uc) are required in both the 6 X 6 and 5 X 5 matrices. The statistical weights for the four-state model are evaluated from the atomic coordinates of the x-ray structures of 26 native proteins. These statistical weights, and the four-state model, are used to develop a procedure to predict the backbone conformations of proteins. Since the prediction of helical and extended conformations is carried out by the procedure described in papers 1-3 of this series, we focus particular attention on chain-reversal conformations in this paper. The conformational-sequence probabilities of finding a residue in h, epsilon, R, S, or c states, and of finding two consecutive residues in a chain-reversal conformation, defined as relative values with respect to their average values over the whole molecule, are calculated for 23 proteins. By comparing these conformational-sequence probabilities to experimental X-ray observations, it was found that, in addition to the prediction of helical and extended conformations (reported in paper 3), 219 chain-reversal regions out of 372 observed by x-ray diffraction studies of 23 proteins were predicted correctly. These results suggest that the assumption of the dominance of short-range interactions in determining chain-reversal (as well as helical or extended) conformations in proteins, on which the predictive scheme is based, is a reasonable one. Finally, in the Appendix, the property of asymmetric nucleation of helical sequences is introduced into the (nearest-neighbor) four-state model.", "contents": "Statistical mechanical treatment of protein conformation. 4. A four-state model for specific-sequence copolymers of amino acids. One-dimensional short-range interaction models for specific-sequence copolymers of amino acids are being developed in this series of papers. In this paper, our earlier three-state model [involving helical (h), extended (epsilon), and coil (or other) (c) states] is extended to a four-state model by preserving the h and epsilon states, introducing the chain-reversal state (R and S), and redefining the c state. This model involves six parameters (wh, vh, vepsilon, vR, vS, and uc) and requires a 6 X 6 statistical weight matrix. A nearest-neighbor approximation of the four-state model is also formulated; it requires a 5 X 5 matrix, involving the same six parameters. By expressing the statistical weights relative to that of the epsilon state, only five parameters (wh, vh, vR, vS, and uc) are required in both the 6 X 6 and 5 X 5 matrices. The statistical weights for the four-state model are evaluated from the atomic coordinates of the x-ray structures of 26 native proteins. These statistical weights, and the four-state model, are used to develop a procedure to predict the backbone conformations of proteins. Since the prediction of helical and extended conformations is carried out by the procedure described in papers 1-3 of this series, we focus particular attention on chain-reversal conformations in this paper. The conformational-sequence probabilities of finding a residue in h, epsilon, R, S, or c states, and of finding two consecutive residues in a chain-reversal conformation, defined as relative values with respect to their average values over the whole molecule, are calculated for 23 proteins. By comparing these conformational-sequence probabilities to experimental X-ray observations, it was found that, in addition to the prediction of helical and extended conformations (reported in paper 3), 219 chain-reversal regions out of 372 observed by x-ray diffraction studies of 23 proteins were predicted correctly. These results suggest that the assumption of the dominance of short-range interactions in determining chain-reversal (as well as helical or extended) conformations in proteins, on which the predictive scheme is based, is a reasonable one. Finally, in the Appendix, the property of asymmetric nucleation of helical sequences is introduced into the (nearest-neighbor) four-state model.", "PMID": 979339}
{"id": "pubmed23n0032_12106", "title": "[PAS-positive substances in endometrial cells during the course of irregular bleeding].", "content": "Cytologic smears of the endometrium in 81 cases of irregular bleeding were analysed for carbohydrates by the modified PAS method after Hotchkiss (1948). The positive issue of the cytochemical reaction was determined semiquantitatively. The intensity of reaction was numerically scored fy differentiating positivity in cells. Statistical analysis of data and the testing of the significance of differences have shown that are statistically highly significant differences in the intensity of reaction between the group of the secretion phase on the one hand and, on the other, the group of the proliferation phase, glandular cystic hyperplasia, and glandular hyperplasia. It has also been found that differences in the intensity of reaction between the group of the proliferation phase and the group of glandular cystic hyperplasia, as well as between the group of glandular cystic hyperplasia and glandular hyperplasia were only accidental.", "contents": "[PAS-positive substances in endometrial cells during the course of irregular bleeding]. Cytologic smears of the endometrium in 81 cases of irregular bleeding were analysed for carbohydrates by the modified PAS method after Hotchkiss (1948). The positive issue of the cytochemical reaction was determined semiquantitatively. The intensity of reaction was numerically scored fy differentiating positivity in cells. Statistical analysis of data and the testing of the significance of differences have shown that are statistically highly significant differences in the intensity of reaction between the group of the secretion phase on the one hand and, on the other, the group of the proliferation phase, glandular cystic hyperplasia, and glandular hyperplasia. It has also been found that differences in the intensity of reaction between the group of the proliferation phase and the group of glandular cystic hyperplasia, as well as between the group of glandular cystic hyperplasia and glandular hyperplasia were only accidental.", "PMID": 979336}
{"id": "pubmed23n0032_12107", "title": "[New radiologico-clinical classification of changes in stress incontinence in women].", "content": "Setting forth their experience in over 800 colpocystographies applied in genital prolapse and stress incontinence, the authors propose an original classification of changes in urinary stress incontinence with a view to unifying clinical and radiological findings. Along with classical, wellknown radiological aspects (urethral vesicalization and the prolapse of the urinary bladder) one new type of changes is described. It is named the slipping prolapse of the urinary bladder and is determined by the deterioration of the urethro-vaginal septum leading to a completely isolated dislocation of the lower urinary organs and the frontal vaginal wall. The combination of these aspects gives the three types and six variants of stress incontinence which, from the clinical point of view, may be manifest, masked, and potential. The pathogenesis of different types of the disease is analysed, as well as the principles of their therapy. The authors plead for the widest possible use of colpocystography in the preoperative preparation on patients, especially in relapses, since the method is simple and harmless, yielding extremely useful informations in the study of the morphotopography of pelvic organs.", "contents": "[New radiologico-clinical classification of changes in stress incontinence in women]. Setting forth their experience in over 800 colpocystographies applied in genital prolapse and stress incontinence, the authors propose an original classification of changes in urinary stress incontinence with a view to unifying clinical and radiological findings. Along with classical, wellknown radiological aspects (urethral vesicalization and the prolapse of the urinary bladder) one new type of changes is described. It is named the slipping prolapse of the urinary bladder and is determined by the deterioration of the urethro-vaginal septum leading to a completely isolated dislocation of the lower urinary organs and the frontal vaginal wall. The combination of these aspects gives the three types and six variants of stress incontinence which, from the clinical point of view, may be manifest, masked, and potential. The pathogenesis of different types of the disease is analysed, as well as the principles of their therapy. The authors plead for the widest possible use of colpocystography in the preoperative preparation on patients, especially in relapses, since the method is simple and harmless, yielding extremely useful informations in the study of the morphotopography of pelvic organs.", "PMID": 979337}
{"id": "pubmed23n0032_12108", "title": "Coronary anatomy.", "content": "The coronary arteries have a serpentine but interdependent relationship to one another. The left artery supplies the majority of the left ventricular myocardium irrespective of right or left arterial \"dominance.\" A coronary artery obstruction begins to assume significance when it approaches 75% of the cross-sectional area of the vessel. Increasing degrees of stenosis impose limitations to blood flow during stress and finally even under resting conditions. Analysis of the arteriogram requires not only knowledge of normal anatomy and its variations but also appreciation of difficulties in distinguishing a normal from an abnormal artery and in quantifying the functional severity of an obvious obstruction.", "contents": "Coronary anatomy. The coronary arteries have a serpentine but interdependent relationship to one another. The left artery supplies the majority of the left ventricular myocardium irrespective of right or left arterial \"dominance.\" A coronary artery obstruction begins to assume significance when it approaches 75% of the cross-sectional area of the vessel. Increasing degrees of stenosis impose limitations to blood flow during stress and finally even under resting conditions. Analysis of the arteriogram requires not only knowledge of normal anatomy and its variations but also appreciation of difficulties in distinguishing a normal from an abnormal artery and in quantifying the functional severity of an obvious obstruction.", "PMID": 979347}
{"id": "pubmed23n0032_12109", "title": "Coronary collaterals.", "content": "Coronary collaterals are probably enlargements of pre-existing channels which respond to local vasodilators and which function whenever pressure differences exist across them. Thus, in human coronary atherosclerosis collaterals are only seen when there is a severe intervening arterial obstruction (in excess of 75%). Coronary collaterals follow epicardial and intramycardial pathways, and the intermediary connections may be at vessels of highly varying caliber. The flow potential of most collateral pathways in man is possibly adequate for segmental myocardial function at lower than normal demands but clearly is inadequate for most, if not all, stressful interventions. In the last analysis, coronary collaterals in man are more an indication of severe regional ischemia (present or potential) than a sign of biological \"compensation'' for a perfusion deficit.", "contents": "Coronary collaterals. Coronary collaterals are probably enlargements of pre-existing channels which respond to local vasodilators and which function whenever pressure differences exist across them. Thus, in human coronary atherosclerosis collaterals are only seen when there is a severe intervening arterial obstruction (in excess of 75%). Coronary collaterals follow epicardial and intramycardial pathways, and the intermediary connections may be at vessels of highly varying caliber. The flow potential of most collateral pathways in man is possibly adequate for segmental myocardial function at lower than normal demands but clearly is inadequate for most, if not all, stressful interventions. In the last analysis, coronary collaterals in man are more an indication of severe regional ischemia (present or potential) than a sign of biological \"compensation'' for a perfusion deficit.", "PMID": 979348}
{"id": "pubmed23n0032_12110", "title": "[Intraoperative cytodiagnosis of ovarian tumors].", "content": "The authors report on their experience in applying rapid intraoperative cytologic analysis in 83 benign and 54 malignant ovarian tumors of various pathohistologic types. The entire procedure, from the receiving of the material, fixing and staining, up to the cytologic diagnosis, did not last longer than 6 minutes. The cytologic findings were negative in all benign tumors except one (99.3%). In cases of malignant tumors the cytologic diagnosis was correct in 49 cases (96.4%). On the basis of cytomorphologic criteria it was also possible to predict the pathohistologic type of most of investigated tumors. The high overall accuracy rate of intraoperative cytologic examination permits the surgeon to make a quick and adequate therapeutic decision concerning the extent of intervention.", "contents": "[Intraoperative cytodiagnosis of ovarian tumors]. The authors report on their experience in applying rapid intraoperative cytologic analysis in 83 benign and 54 malignant ovarian tumors of various pathohistologic types. The entire procedure, from the receiving of the material, fixing and staining, up to the cytologic diagnosis, did not last longer than 6 minutes. The cytologic findings were negative in all benign tumors except one (99.3%). In cases of malignant tumors the cytologic diagnosis was correct in 49 cases (96.4%). On the basis of cytomorphologic criteria it was also possible to predict the pathohistologic type of most of investigated tumors. The high overall accuracy rate of intraoperative cytologic examination permits the surgeon to make a quick and adequate therapeutic decision concerning the extent of intervention.", "PMID": 979335}
{"id": "pubmed23n0032_12111", "title": "Physiology of myocardial blood flow and metabolism.", "content": "In this chapter the normal functioning of the coronary circulation has been described with major emphasis placed on the hydraulic and small vessel resistance factors in its regulation. The impact of various forms of disease to compromise effective delivery of blood flow either in general or segmentally has also been stressed. The vulnerability of the deep or subendocardial layers to ischemia has been emphasized. The distinction has been drawn between primary and secondary vasodilatation, the primary type representing effects on the arteriole, irrespective of effects on myocardial mechanical and metabolic activity, while the secondary type represents changes in response to changes in the metabolic rate. An interrelationship has been demonstrated among the several factors which regulate blood supply and affect myocardial mechanical activity.", "contents": "Physiology of myocardial blood flow and metabolism. In this chapter the normal functioning of the coronary circulation has been described with major emphasis placed on the hydraulic and small vessel resistance factors in its regulation. The impact of various forms of disease to compromise effective delivery of blood flow either in general or segmentally has also been stressed. The vulnerability of the deep or subendocardial layers to ischemia has been emphasized. The distinction has been drawn between primary and secondary vasodilatation, the primary type representing effects on the arteriole, irrespective of effects on myocardial mechanical and metabolic activity, while the secondary type represents changes in response to changes in the metabolic rate. An interrelationship has been demonstrated among the several factors which regulate blood supply and affect myocardial mechanical activity.", "PMID": 979349}
{"id": "pubmed23n0032_12112", "title": "The high rate of appendectomy in Japan.", "content": "During the past 25 years in Japan, a change has occurred in the number of appendectomies performed. The results of an investigation in a private (N) hospital showed that 63 per cent of the operations was for \"catarrhal appendicitis\" that had no inflammatory findings. This \"catarrhal type\" was found at the highest level in young women. We also investigated the rate of appendectomy by age, and computed the yearly rate increase. It was found that a significant increase in the operation rate began about 1950, and the annual rate thereafter amounted to about 0.75 per cent-or about threefold that in England. This annual increase was attributed to the number of cases in which no inflammation was found in the operated appendix. We discussed the cause and concluded that this high rate of appendectomy is believed to be secondary to the fee-for-service system in the medical insurance programs that were instituted in Japan after World War II.", "contents": "The high rate of appendectomy in Japan. During the past 25 years in Japan, a change has occurred in the number of appendectomies performed. The results of an investigation in a private (N) hospital showed that 63 per cent of the operations was for \"catarrhal appendicitis\" that had no inflammatory findings. This \"catarrhal type\" was found at the highest level in young women. We also investigated the rate of appendectomy by age, and computed the yearly rate increase. It was found that a significant increase in the operation rate began about 1950, and the annual rate thereafter amounted to about 0.75 per cent-or about threefold that in England. This annual increase was attributed to the number of cases in which no inflammation was found in the operated appendix. We discussed the cause and concluded that this high rate of appendectomy is believed to be secondary to the fee-for-service system in the medical insurance programs that were instituted in Japan after World War II.", "PMID": 979396}
{"id": "pubmed23n0032_12113", "title": "Common manifestations of degenerative arterial disease.", "content": "The major focus of preventive medicine in the aged must be on the clinical consequences of peripheral arteriosclerosis rather than the disease process itself. Treating risk factors may be of some limited benefit. However, of most crucial importance is careful attention to premonitory symptoms and the state of the peripheral pulses including the abdominal aorta.", "contents": "Common manifestations of degenerative arterial disease. The major focus of preventive medicine in the aged must be on the clinical consequences of peripheral arteriosclerosis rather than the disease process itself. Treating risk factors may be of some limited benefit. However, of most crucial importance is careful attention to premonitory symptoms and the state of the peripheral pulses including the abdominal aorta.", "PMID": 979398}
{"id": "pubmed23n0032_12114", "title": "[Early complications and long-term results after total gastrectomy. A contribution to finding a method of gastric replacement, based on own observations (author's transl)].", "content": "In the years 1963 up to 1973 in the Department of Surgery of the University of D\u00fcsseldorf a total gastrectomy was performed in 53 patients. For reconstitution of gastrointestinal continuity in 22 cases we interposed a single jejunal loop between the esophagus and duodenum, in 15 patients we performed an esophagojejunostomy with a long enteroanastomosis between the afferent and efferent loop. The procedure described by Tomoda was done in 11 patients. An esophagojejunostomy Roux-en-Y we used in 3 and an esophagoduodenostomy in only 2 cases. The hospital mortality was 21%, the cause of which were predominantly complications of the lung. A dehiscence of an anastomosis never was a cause of death. A follow-up we could perform in 15 patients. On the basis of clinical, roentgenological and chemical data received in the follow-up we prefer at this time the interposition of a single jejunal loop, the length of which should be no less than 40 cm.", "contents": "[Early complications and long-term results after total gastrectomy. A contribution to finding a method of gastric replacement, based on own observations (author's transl)]. In the years 1963 up to 1973 in the Department of Surgery of the University of D\u00fcsseldorf a total gastrectomy was performed in 53 patients. For reconstitution of gastrointestinal continuity in 22 cases we interposed a single jejunal loop between the esophagus and duodenum, in 15 patients we performed an esophagojejunostomy with a long enteroanastomosis between the afferent and efferent loop. The procedure described by Tomoda was done in 11 patients. An esophagojejunostomy Roux-en-Y we used in 3 and an esophagoduodenostomy in only 2 cases. The hospital mortality was 21%, the cause of which were predominantly complications of the lung. A dehiscence of an anastomosis never was a cause of death. A follow-up we could perform in 15 patients. On the basis of clinical, roentgenological and chemical data received in the follow-up we prefer at this time the interposition of a single jejunal loop, the length of which should be no less than 40 cm.", "PMID": 979472}
{"id": "pubmed23n0032_12115", "title": "[The surgical treatment of villous tumours of the large bowel (author's transl)].", "content": "The treatment of 12 patients with villous tumours of the rectum is described. In benign lesions a sphincter conserving operation should be employed. With the technique of submucosal local excision per anum which was described by A.G. Parks it is possible to remove large villous tumours of the rectum. If there is malignant change with invasion of the muscularis muscosae which is found in about 20% of the cases resection therapy is necessary.", "contents": "[The surgical treatment of villous tumours of the large bowel (author's transl)]. The treatment of 12 patients with villous tumours of the rectum is described. In benign lesions a sphincter conserving operation should be employed. With the technique of submucosal local excision per anum which was described by A.G. Parks it is possible to remove large villous tumours of the rectum. If there is malignant change with invasion of the muscularis muscosae which is found in about 20% of the cases resection therapy is necessary.", "PMID": 979473}
{"id": "pubmed23n0032_12116", "title": "[Uncusectomy and uncoforaminectomy according to Jung. Technique, indications and results (author's transl)].", "content": "The authors described the technique of uncusectomy and uncoforaminectomia according to A. Jung. The Jung-operation can be defined as the opening of the intervertebral foramina through the anterior approach. The aims of this operation are the liberation of a displaced or narrowed vertebral artery and to free compressed or irritated nerve roots by means of opening and widening the intervertebral foramina from the front. This report is based on 180 such operations performed by A. Jung and colleagues. The operation is indicated whenever cervical uncarthrosis leads to a vertebrobasilar insufficiency syndrome or a cervical-brachial irritation syndrome. Both syndromes commonly occur together. The results of operative treatment are analysed and the success rate lies around 80%.", "contents": "[Uncusectomy and uncoforaminectomy according to Jung. Technique, indications and results (author's transl)]. The authors described the technique of uncusectomy and uncoforaminectomia according to A. Jung. The Jung-operation can be defined as the opening of the intervertebral foramina through the anterior approach. The aims of this operation are the liberation of a displaced or narrowed vertebral artery and to free compressed or irritated nerve roots by means of opening and widening the intervertebral foramina from the front. This report is based on 180 such operations performed by A. Jung and colleagues. The operation is indicated whenever cervical uncarthrosis leads to a vertebrobasilar insufficiency syndrome or a cervical-brachial irritation syndrome. Both syndromes commonly occur together. The results of operative treatment are analysed and the success rate lies around 80%.", "PMID": 979474}
{"id": "pubmed23n0032_12117", "title": "[Complications of the vagotomy during the operation and in the early postoperative period (author's transl)].", "content": "426 subdiaphragmatic vagotomy operations were performed by the authors. On basis of the results obtained they give full details of the complications arising in the course and in the early postoperative period after vagotomy, and also of the possibilities preventing these complications. They call the attention to the more frequent injuries of the spleen and the oesophagus, and to the increasing number of the subphrenic abscess. Finally, they emphasize the low mortality rate of the vagotomy in comparison to the traditional gastric resections.", "contents": "[Complications of the vagotomy during the operation and in the early postoperative period (author's transl)]. 426 subdiaphragmatic vagotomy operations were performed by the authors. On basis of the results obtained they give full details of the complications arising in the course and in the early postoperative period after vagotomy, and also of the possibilities preventing these complications. They call the attention to the more frequent injuries of the spleen and the oesophagus, and to the increasing number of the subphrenic abscess. Finally, they emphasize the low mortality rate of the vagotomy in comparison to the traditional gastric resections.", "PMID": 979475}
{"id": "pubmed23n0032_12118", "title": "[Massive hemorrhage caused by acute solitary gastric erosion Dieulafoy. Change of prognosis due to preoperative endoscopy (author's transl)].", "content": "In this report of three successfully operated cases of acute solitary gastric erosion--Ulcus Dieulafoy (U.D.), the anatomical and clinical characteristics of this rare gastric lesion are discussed. Due credit is given to reports of the literature. As born out by experience, conservative treatment of bleeding U.D. is usually lethal. Judged from statistical evaluation, surgical intervention has proven to be unsuccessful in many cases. This is directly related to the problem of intraoperative localisation, which, in turn, may lead to illadvised procedures. With acurate localisation of the lesion, surgical management yields good to excellent results. Acute endoscopy as a routine procedure in gastrointestinal hemorrhage in our experience is the key to success, leading to a possibly decisive change in the prognosis of U.D.", "contents": "[Massive hemorrhage caused by acute solitary gastric erosion Dieulafoy. Change of prognosis due to preoperative endoscopy (author's transl)]. In this report of three successfully operated cases of acute solitary gastric erosion--Ulcus Dieulafoy (U.D.), the anatomical and clinical characteristics of this rare gastric lesion are discussed. Due credit is given to reports of the literature. As born out by experience, conservative treatment of bleeding U.D. is usually lethal. Judged from statistical evaluation, surgical intervention has proven to be unsuccessful in many cases. This is directly related to the problem of intraoperative localisation, which, in turn, may lead to illadvised procedures. With acurate localisation of the lesion, surgical management yields good to excellent results. Acute endoscopy as a routine procedure in gastrointestinal hemorrhage in our experience is the key to success, leading to a possibly decisive change in the prognosis of U.D.", "PMID": 979476}
{"id": "pubmed23n0032_12119", "title": "[Experimental treatment of the lesion of the flexor tendon, by means of tendon sheath substitution using a vein and diphasic flexor tendon plasty. A new method for the reconstruction of peritendinous slipping surfaces (author's transl)].", "content": "In dog experiments the surgical treatment of flexor tendons is discussed by the authors: the lesions of the tendons have been repaired according to the method of Paneva-Holevitch, with biphasic flexor tendon plasty; and for the substitution of the injured tendon sheath and the peritendinous slipping surfaces freely transplanted, autologous vein has been used. In the preparations removed after 8 weeks both the macroscopic and microscopic examinations showed satisfactory vascularity of the vein used as tendon sheath and of the tendinous tissue, as well as normal tissue structure. Between the vein and the tendon only small, ligamentous adhesions developed. On the basis of the good results obtained in experimental examinations the method is applied by the authors also in men with satisfactory result.", "contents": "[Experimental treatment of the lesion of the flexor tendon, by means of tendon sheath substitution using a vein and diphasic flexor tendon plasty. A new method for the reconstruction of peritendinous slipping surfaces (author's transl)]. In dog experiments the surgical treatment of flexor tendons is discussed by the authors: the lesions of the tendons have been repaired according to the method of Paneva-Holevitch, with biphasic flexor tendon plasty; and for the substitution of the injured tendon sheath and the peritendinous slipping surfaces freely transplanted, autologous vein has been used. In the preparations removed after 8 weeks both the macroscopic and microscopic examinations showed satisfactory vascularity of the vein used as tendon sheath and of the tendinous tissue, as well as normal tissue structure. Between the vein and the tendon only small, ligamentous adhesions developed. On the basis of the good results obtained in experimental examinations the method is applied by the authors also in men with satisfactory result.", "PMID": 979477}
{"id": "pubmed23n0032_12120", "title": "[Experimental bone replacement with resorbable calcium phosphate ceramic (author's transl)].", "content": "Compact cylindrical implants (5 x 15.5 mm) of seven calcium phosphate ceramics of different formulations that had been implanted for 6 weeks without fixation in the tibia of dogs were examined histologically. The tissue compatibility of the implant turned out to be dependent on the mineralogical and chemical composition of the material: the ceramic material was biocompatible at a CaO/P2O5 ratio between 2:1 and 4:1, the optimum ratio being about 3:1 (tricalcium phosphate). In addition, cylindrical tetracalcium phosphate implatns with an entirely porous structure were implanted in the same manner. After 6 months, they were resorbed to a minor extent. Implants of different calcium phosphate mixtures, on the other hand, were resorbed to a large extend after the same time. The ceramic material was replaced by mineralised bone tissue which had formed directly on the ceramic implant as well as within the pores. There were no foreign body reactions. Investigations with segment-shaped implants which were implanted in the tibia of dogs and fixed with AO-plates or splints extra-cutaneously for about 8-10 weeks, provided information abouth the maximum stress that can be borne by the implants. While implants with a porosity of 75 percent did not sustain the stress after the fixation had been removed, those with a porosity of 45 percent could be subjected to physiological stress after removal of the splints.", "contents": "[Experimental bone replacement with resorbable calcium phosphate ceramic (author's transl)]. Compact cylindrical implants (5 x 15.5 mm) of seven calcium phosphate ceramics of different formulations that had been implanted for 6 weeks without fixation in the tibia of dogs were examined histologically. The tissue compatibility of the implant turned out to be dependent on the mineralogical and chemical composition of the material: the ceramic material was biocompatible at a CaO/P2O5 ratio between 2:1 and 4:1, the optimum ratio being about 3:1 (tricalcium phosphate). In addition, cylindrical tetracalcium phosphate implatns with an entirely porous structure were implanted in the same manner. After 6 months, they were resorbed to a minor extent. Implants of different calcium phosphate mixtures, on the other hand, were resorbed to a large extend after the same time. The ceramic material was replaced by mineralised bone tissue which had formed directly on the ceramic implant as well as within the pores. There were no foreign body reactions. Investigations with segment-shaped implants which were implanted in the tibia of dogs and fixed with AO-plates or splints extra-cutaneously for about 8-10 weeks, provided information abouth the maximum stress that can be borne by the implants. While implants with a porosity of 75 percent did not sustain the stress after the fixation had been removed, those with a porosity of 45 percent could be subjected to physiological stress after removal of the splints.", "PMID": 979478}
{"id": "pubmed23n0032_12121", "title": "[Epithelial dysplasia in ulcerative colitis. Histological possibility for the early diagnosis of \"colitis\"-carcinoma (author's transl)].", "content": "From 249 histologically examined cases with ulcerative colitis (within the years 1967 to 1974) 20 cases were selected, which had an onset of the disease before the 20th year of age and/or a duration of more than 10 years. These cases were checked-up for \"prae-cancerous\" epithelial dysplasia. Four of these 20 cases showed medium to severe epithelial dysplasia together with carcinoma (= 1.61% of the entire collective). In 2 cases low grade to severe dysplasia occurred in different parts of the colon without demonstrable carcinoma. In 1 case epidermoid epithelial metaplasia were found in the splenic flexure and in the rectum. The biological significance of these metaplasia remains unclear. With reference to the formal pathogenesis these lesions are probably \"indirect\" metaplasia. The early diagnosis of \"colitis\"-carcinoma can be improved by systematic colonoscopic examinations resp. by colonoscopic biopsies. \"Prae-cancerous\" epithelial dysplasias, which occur in a high rate together with carcinoma, are found not only in the rectum but also in other parts of the colon. Multiple biopsies from different parts of the colon as well as the rectum would thus seem to be desirable if mucosal sampling is to be employed as a screening test.", "contents": "[Epithelial dysplasia in ulcerative colitis. Histological possibility for the early diagnosis of \"colitis\"-carcinoma (author's transl)]. From 249 histologically examined cases with ulcerative colitis (within the years 1967 to 1974) 20 cases were selected, which had an onset of the disease before the 20th year of age and/or a duration of more than 10 years. These cases were checked-up for \"prae-cancerous\" epithelial dysplasia. Four of these 20 cases showed medium to severe epithelial dysplasia together with carcinoma (= 1.61% of the entire collective). In 2 cases low grade to severe dysplasia occurred in different parts of the colon without demonstrable carcinoma. In 1 case epidermoid epithelial metaplasia were found in the splenic flexure and in the rectum. The biological significance of these metaplasia remains unclear. With reference to the formal pathogenesis these lesions are probably \"indirect\" metaplasia. The early diagnosis of \"colitis\"-carcinoma can be improved by systematic colonoscopic examinations resp. by colonoscopic biopsies. \"Prae-cancerous\" epithelial dysplasias, which occur in a high rate together with carcinoma, are found not only in the rectum but also in other parts of the colon. Multiple biopsies from different parts of the colon as well as the rectum would thus seem to be desirable if mucosal sampling is to be employed as a screening test.", "PMID": 979479}
{"id": "pubmed23n0032_12122", "title": "[A panel on vascular surgery (author's transl)].", "content": "For this second panel by correspondence, we have asked 3 distinguished experts to answer 7 questions and to comment on 3 case histories concerning vascular surgery. Although a direct discussion between the participants was not possible an impressive degree of agreement is here being demonstrated.", "contents": "[A panel on vascular surgery (author's transl)]. For this second panel by correspondence, we have asked 3 distinguished experts to answer 7 questions and to comment on 3 case histories concerning vascular surgery. Although a direct discussion between the participants was not possible an impressive degree of agreement is here being demonstrated.", "PMID": 979480}
{"id": "pubmed23n0032_12123", "title": "[Compression of the celiac trunk (author's transl)].", "content": "Besides the vascular changes caused by arteriosclerosis the compression stenosis is the most frequent form of the isolated restriction of the arteria coeliaca. The compression of the arteria coeliaca, caused by the ligamentum arcuatum medianum or a fribromatous ganglion tissue, can cause complaints similar to the symptoms of angina abdominalis: pains in the epigastrium, postprandial pains, loss of weight, nausea/vomiting, diarrhea, 93% of the patients with severe vascular compression have an abdominal vascular murmur. Of 31 patients with angiographically proved compression stenosis, 11 patients suffering from occlusion or intense stenosis had to be operated because of heavy complaints. The angiographic and intraoperative findings allow the conclusion that there is a connection between the extent of the stenosis and the clinical appearance. The decompression of the arteria coeliaca, in other words the detachment of the compressing tissue leads to total complaintlessness in 83% of the patients. If the arteria coeliaca is hypoplastic a vessel widening or a bypass operation is necessary to establish normal blood circulation in the epigastric organs.", "contents": "[Compression of the celiac trunk (author's transl)]. Besides the vascular changes caused by arteriosclerosis the compression stenosis is the most frequent form of the isolated restriction of the arteria coeliaca. The compression of the arteria coeliaca, caused by the ligamentum arcuatum medianum or a fribromatous ganglion tissue, can cause complaints similar to the symptoms of angina abdominalis: pains in the epigastrium, postprandial pains, loss of weight, nausea/vomiting, diarrhea, 93% of the patients with severe vascular compression have an abdominal vascular murmur. Of 31 patients with angiographically proved compression stenosis, 11 patients suffering from occlusion or intense stenosis had to be operated because of heavy complaints. The angiographic and intraoperative findings allow the conclusion that there is a connection between the extent of the stenosis and the clinical appearance. The decompression of the arteria coeliaca, in other words the detachment of the compressing tissue leads to total complaintlessness in 83% of the patients. If the arteria coeliaca is hypoplastic a vessel widening or a bypass operation is necessary to establish normal blood circulation in the epigastric organs.", "PMID": 979481}
{"id": "pubmed23n0032_12124", "title": "[The development of diverticulosis and diverticulitis (author's transl)].", "content": "The cause of colonic diverticula is myostatic spasm. A low-residue diet contributes to this state as transit time through the bowel is slowed. Information gathered in 102 cases was analysed. The smooth muscle architecture of specially prepared operative specimens of diverticulosis were examined. In every case an asymmetric contracture of the smooth musculature led to the formation of gaps. Here the mucosa and muscularis mucosae can protrude through the bowel wall. The muscular spasm is responsible for incarceration of the diverticula. Inflammatory diverticulitis is compared to appendicitis.", "contents": "[The development of diverticulosis and diverticulitis (author's transl)]. The cause of colonic diverticula is myostatic spasm. A low-residue diet contributes to this state as transit time through the bowel is slowed. Information gathered in 102 cases was analysed. The smooth muscle architecture of specially prepared operative specimens of diverticulosis were examined. In every case an asymmetric contracture of the smooth musculature led to the formation of gaps. Here the mucosa and muscularis mucosae can protrude through the bowel wall. The muscular spasm is responsible for incarceration of the diverticula. Inflammatory diverticulitis is compared to appendicitis.", "PMID": 979482}
{"id": "pubmed23n0032_12125", "title": "[A special closure system of the gastrointestinal tract of the rat (author's transl)].", "content": "As a result of animal experiments, morpho-histologic and surgical findings, the presence of a new closure system of the gastrointestinal tract is described. The mode of action is different from that of all the known muscle sphincters. It is found in the ascending colon of the rat and consists of a specially arranged and adjustable mucosal folds with aunique vascularisation. A functional unit which comosed of a pair of folds and a vascular plexus constitute the valvular mechanism. We, therefore, consider appropriate to call it the Angioplical Closure System. Surgical reversion of even a very small portion of the system causes a lethal ileus.", "contents": "[A special closure system of the gastrointestinal tract of the rat (author's transl)]. As a result of animal experiments, morpho-histologic and surgical findings, the presence of a new closure system of the gastrointestinal tract is described. The mode of action is different from that of all the known muscle sphincters. It is found in the ascending colon of the rat and consists of a specially arranged and adjustable mucosal folds with aunique vascularisation. A functional unit which comosed of a pair of folds and a vascular plexus constitute the valvular mechanism. We, therefore, consider appropriate to call it the Angioplical Closure System. Surgical reversion of even a very small portion of the system causes a lethal ileus.", "PMID": 979483}
{"id": "pubmed23n0032_12126", "title": "[Appearance of a localized peritoneal mesothelioma seven years after resection of pericardium on account of localized pericardial mesothelioma (author's transl)].", "content": "For the first time appearance of a localized benign pericardial mesothelioma and peritoneal mesothelioma in one and the same patient is described. The experiences made in the course of the treatment, the diagnostic difficulties, symptomatology, histology and prognosis are discussed.", "contents": "[Appearance of a localized peritoneal mesothelioma seven years after resection of pericardium on account of localized pericardial mesothelioma (author's transl)]. For the first time appearance of a localized benign pericardial mesothelioma and peritoneal mesothelioma in one and the same patient is described. The experiences made in the course of the treatment, the diagnostic difficulties, symptomatology, histology and prognosis are discussed.", "PMID": 979484}
{"id": "pubmed23n0032_12127", "title": "Prevention of stress ulcer by somatostatin in rats.", "content": "In animal experiments it was examined, to what extent prophylactic application of Somatostatin is able to prevent the stress-induced ulcer of the rat. 32 male Wistar rats were exposed to a combined immobilisation-hypoxia-stress. Somatostatin was given intraperitoneally as bolus and intravenously as a continuous infusion. The results indicated that Somatostatin lowers significantly the incidence rate of stress-induced gastric ulcers in the rat.", "contents": "Prevention of stress ulcer by somatostatin in rats. In animal experiments it was examined, to what extent prophylactic application of Somatostatin is able to prevent the stress-induced ulcer of the rat. 32 male Wistar rats were exposed to a combined immobilisation-hypoxia-stress. Somatostatin was given intraperitoneally as bolus and intravenously as a continuous infusion. The results indicated that Somatostatin lowers significantly the incidence rate of stress-induced gastric ulcers in the rat.", "PMID": 979485}
{"id": "pubmed23n0032_12128", "title": "[Pathogenesis and clinic of pancreatic fistulas (author's transl)].", "content": "At the Surgical University Clinik of Mainz 53 patients with a pancreatic fistula have been observed in 12 years (1964-1975). Most frequently these fistulas occurred after operations for acute pancreatitis (20%), after exstirpation of insulinomas (20%) or after trauma (19%). In 44 cases an external and in 9 cases an internal fistula was seen. Pathogenetic differentiation in tryptic and non-tryptic fistulas allows some prognostic conclusions. So tryptic fistulas are often complicated by internal fistulas with preference for the colon and must be operated more often. In 15 patients the persisting pancreatic fistula has been treated operatively. In seven cases pancreatic resection and in 4 cases the resection of a part of the colon was necessary. Conservatively treated fistulas in most cases closed within 6 weeks.", "contents": "[Pathogenesis and clinic of pancreatic fistulas (author's transl)]. At the Surgical University Clinik of Mainz 53 patients with a pancreatic fistula have been observed in 12 years (1964-1975). Most frequently these fistulas occurred after operations for acute pancreatitis (20%), after exstirpation of insulinomas (20%) or after trauma (19%). In 44 cases an external and in 9 cases an internal fistula was seen. Pathogenetic differentiation in tryptic and non-tryptic fistulas allows some prognostic conclusions. So tryptic fistulas are often complicated by internal fistulas with preference for the colon and must be operated more often. In 15 patients the persisting pancreatic fistula has been treated operatively. In seven cases pancreatic resection and in 4 cases the resection of a part of the colon was necessary. Conservatively treated fistulas in most cases closed within 6 weeks.", "PMID": 979486}
{"id": "pubmed23n0032_12129", "title": "Experiences in lateral pharyngotomy.", "content": "Lateral pharyngotomy offers good exposure for the removal of many laryngeal and hypopharyngeal tumors. The simplified operation I present avoids damage to voice, deglutition or airway. This approach can be expanded to reach almost any tumor of the pharynx and radical neck dissection can be incorporated. The cosmetic result has been satisfactory, but if individual consideration prompts it, a more horizontal skin-fold incision can easily be substituted.", "contents": "Experiences in lateral pharyngotomy. Lateral pharyngotomy offers good exposure for the removal of many laryngeal and hypopharyngeal tumors. The simplified operation I present avoids damage to voice, deglutition or airway. This approach can be expanded to reach almost any tumor of the pharynx and radical neck dissection can be incorporated. The cosmetic result has been satisfactory, but if individual consideration prompts it, a more horizontal skin-fold incision can easily be substituted.", "PMID": 979488}
{"id": "pubmed23n0032_12130", "title": "Thorium dioxide granuloma of the neck: a report of four cases.", "content": "Four patients with Thorotrast granuloma are reported. The first patient had a radical neck dissection performed; however, postoperative hemorrhage and fistula occurred with a six-month delay in healing. The granuloma in this patient had totally occluded his carotid system with a greatly enlarged vertebral artery. A second patient presented with spontaneous bleeding in the neck from a granuloma followed by encephalomalacia, hemiparesis and aphasia. The third and fourth patients presented as hoarseness with a hard mass in the neck. All four patients had Thorotrast in the liver and spleen. The latter three had laryngoscopies and incisional neck biopsies as their only surgical treatment. Neck malignancy from Thorotrast is very rare. Despite legal decisions suggesting removal and similar suggestions in the literature, the authors feel only small extravasations have had uncomplicated operations. Neck dissection is rarely indicated. Hoarseness can be improved by vocal cord injection. These patients, of course, must be followed periodically as any other tumor, checking the neck mass, liver, and carotid circulation.", "contents": "Thorium dioxide granuloma of the neck: a report of four cases. Four patients with Thorotrast granuloma are reported. The first patient had a radical neck dissection performed; however, postoperative hemorrhage and fistula occurred with a six-month delay in healing. The granuloma in this patient had totally occluded his carotid system with a greatly enlarged vertebral artery. A second patient presented with spontaneous bleeding in the neck from a granuloma followed by encephalomalacia, hemiparesis and aphasia. The third and fourth patients presented as hoarseness with a hard mass in the neck. All four patients had Thorotrast in the liver and spleen. The latter three had laryngoscopies and incisional neck biopsies as their only surgical treatment. Neck malignancy from Thorotrast is very rare. Despite legal decisions suggesting removal and similar suggestions in the literature, the authors feel only small extravasations have had uncomplicated operations. Neck dissection is rarely indicated. Hoarseness can be improved by vocal cord injection. These patients, of course, must be followed periodically as any other tumor, checking the neck mass, liver, and carotid circulation.", "PMID": 979489}
{"id": "pubmed23n0032_12131", "title": "Intact canal wall tympanoplasty in the management of cholesteatoma.", "content": "There are presently two schools of thought on the proper surgical management of cholesteatoma associated with chronic ear disease: one feels that the ear should be exteriorized and left \"open\"; the other takes the position that the disease can be removed, and the ear left in its normal anatomical and functional state. The latter group are known to favor a \"closed\" or more conservative procedure. The purpose of this paper is to review a series of patients who have had cholesteatoma managed by an intact canal wall procedure that prevents a postoperative cavity. Out of a series of 590 chronic ear surgeries performed between January 1, 1970, and December 31, 1974, there were 179 done for cholesteatoma (30 percent). There were 153 patients, 26 of whom had bilateral disease. Twenty-three patients were lost to follow-up, giving an overall total of 154 surgeries with from one to five-year information. The overall follow-up rate was 85 percent. Recurrent cholesteatoma was the most bothersome complication and occurred in 14 percent of the series. Residual cholesteatoma was managed by doing all procedures in two stages. The authors feel that the intact canal wall tympanoplasty is a procedure that will gain acceptance and will be more widely used in the future.", "contents": "Intact canal wall tympanoplasty in the management of cholesteatoma. There are presently two schools of thought on the proper surgical management of cholesteatoma associated with chronic ear disease: one feels that the ear should be exteriorized and left \"open\"; the other takes the position that the disease can be removed, and the ear left in its normal anatomical and functional state. The latter group are known to favor a \"closed\" or more conservative procedure. The purpose of this paper is to review a series of patients who have had cholesteatoma managed by an intact canal wall procedure that prevents a postoperative cavity. Out of a series of 590 chronic ear surgeries performed between January 1, 1970, and December 31, 1974, there were 179 done for cholesteatoma (30 percent). There were 153 patients, 26 of whom had bilateral disease. Twenty-three patients were lost to follow-up, giving an overall total of 154 surgeries with from one to five-year information. The overall follow-up rate was 85 percent. Recurrent cholesteatoma was the most bothersome complication and occurred in 14 percent of the series. Residual cholesteatoma was managed by doing all procedures in two stages. The authors feel that the intact canal wall tympanoplasty is a procedure that will gain acceptance and will be more widely used in the future.", "PMID": 979490}
{"id": "pubmed23n0032_12132", "title": "Papillomas of the larynx and pharynx: two case reports.", "content": "Two unusual cases of papillomas are presented: the first is that of a young boy with extensive papillomas of the larynx who was treated successfully without tracheotomy using endolaryngeal surgery and steroids at the time of each procedure. This case adds to the clinical evidence that the most probable cause of juvenile papillomas is a virus, since the mother had condyloma acuminata during pregnancy and at term. The second case is that of a young girl with rapidly recurring papillomas of the mouth and pharynx who was treated successfully with an autogenous vaccine and cryosurgery after other measures failed. This case demonstrates the effectiveness of cryosurgery in the treatment of papillomas and the possible efficacy of the autogenous vaccine suggests a viral etiology for papillomas of the pharynx and larynx.", "contents": "Papillomas of the larynx and pharynx: two case reports. Two unusual cases of papillomas are presented: the first is that of a young boy with extensive papillomas of the larynx who was treated successfully without tracheotomy using endolaryngeal surgery and steroids at the time of each procedure. This case adds to the clinical evidence that the most probable cause of juvenile papillomas is a virus, since the mother had condyloma acuminata during pregnancy and at term. The second case is that of a young girl with rapidly recurring papillomas of the mouth and pharynx who was treated successfully with an autogenous vaccine and cryosurgery after other measures failed. This case demonstrates the effectiveness of cryosurgery in the treatment of papillomas and the possible efficacy of the autogenous vaccine suggests a viral etiology for papillomas of the pharynx and larynx.", "PMID": 979491}
{"id": "pubmed23n0032_12133", "title": "Temporal bone findings in Pierre Robin syndrome.", "content": "This is the first complete report on the histopathologic study of the temporal bones from an infant with a well-documented Pierre Robin syndrome (micrognathia, glossoptosis and cleft palate), demonstrating multiple middle and inner ear anomalies. The anomalies are basically architectural malformations rather than neutral or end organ developmental anomalies. The anomalies in this case, except for a few points, are somewhat similar in both ears. Multiple anomalies include: abnormal narrowing of the crus commune-utricle junction, superiorly located crus commune and posterior semicircular canal, underdeveloped modiolus, absence of the bony septum between the middle and apical coil (existence of scala communis in left ear), abnormally small internal auditory meatus, and abnormal direction of internal auditory canal, large cartilaginous mass around the superior semicircular canal and in the tympanic end of the fissula ante fenestram, small facial nerve, large facial bony canal dehiscence, anomalic stapes, etc.", "contents": "Temporal bone findings in Pierre Robin syndrome. This is the first complete report on the histopathologic study of the temporal bones from an infant with a well-documented Pierre Robin syndrome (micrognathia, glossoptosis and cleft palate), demonstrating multiple middle and inner ear anomalies. The anomalies are basically architectural malformations rather than neutral or end organ developmental anomalies. The anomalies in this case, except for a few points, are somewhat similar in both ears. Multiple anomalies include: abnormal narrowing of the crus commune-utricle junction, superiorly located crus commune and posterior semicircular canal, underdeveloped modiolus, absence of the bony septum between the middle and apical coil (existence of scala communis in left ear), abnormally small internal auditory meatus, and abnormal direction of internal auditory canal, large cartilaginous mass around the superior semicircular canal and in the tympanic end of the fissula ante fenestram, small facial nerve, large facial bony canal dehiscence, anomalic stapes, etc.", "PMID": 979492}
{"id": "pubmed23n0032_12134", "title": "Zinc therapy in otology.", "content": "At a recent panel on Otology, I asked the audience for a show of hands of those using Zinc for delayed healing and granulations. It was surprising to note that not more than 4 physicians out of 100 had tried Zinc. The need to report our encouraging results was apparent. From 1971 to 1975, 544 tympanoplasties and 122 mastoidectomies were studied for delayed healing due to granulations. Forty-six patients were found to have resistant granulations. Thirty-three of these healed within two weeks of therapy, 10 more required a total of four weeks for healing, and three did not respond well. Sixteen patients had recurrence when the medication was terminated as soon as healing had occurred but responded well when the medication was continued for four weeks after healing was complete. Five patients had nausea, which subsided when the dosage was reduced from the usual 200 mg. of Zinc Sulfate, three times daily with meals to 100 mg., t.i.d. or b.i.d. One patient developed mild urticaria. Zinc therapy is apparently indicated in granulomata of the ear when healing does not occur with conventional therapy, especially in the post-operative patient; however, it will not suffice when there is massive involvement of the mastoid or middle ear, where surgical removal is indicated.", "contents": "Zinc therapy in otology. At a recent panel on Otology, I asked the audience for a show of hands of those using Zinc for delayed healing and granulations. It was surprising to note that not more than 4 physicians out of 100 had tried Zinc. The need to report our encouraging results was apparent. From 1971 to 1975, 544 tympanoplasties and 122 mastoidectomies were studied for delayed healing due to granulations. Forty-six patients were found to have resistant granulations. Thirty-three of these healed within two weeks of therapy, 10 more required a total of four weeks for healing, and three did not respond well. Sixteen patients had recurrence when the medication was terminated as soon as healing had occurred but responded well when the medication was continued for four weeks after healing was complete. Five patients had nausea, which subsided when the dosage was reduced from the usual 200 mg. of Zinc Sulfate, three times daily with meals to 100 mg., t.i.d. or b.i.d. One patient developed mild urticaria. Zinc therapy is apparently indicated in granulomata of the ear when healing does not occur with conventional therapy, especially in the post-operative patient; however, it will not suffice when there is massive involvement of the mastoid or middle ear, where surgical removal is indicated.", "PMID": 979493}
{"id": "pubmed23n0032_12135", "title": "Current adjuncts for surgery of the sphenoid sinus and pituitary gland.", "content": "Surgical intervention in the region of the sphenoid sinus pituitary gland requires extreme precision because of enveloping vital structures. The rhinologic approach to the pituitary gland, once abandoned due to limited visibility and the risk of meningitis, has experienced a renaissance as a result of recent medical and technical advances. Antibiotics have almost eliminated the risk of meningitis, and the operating microscope and televised radiofluoroscope now provide sufficient illumination, magnification, and orientation that injury to nearby structures can be avoided. A mid-line rhinologic approach provides ample exposure and further minimizes operative hazards.", "contents": "Current adjuncts for surgery of the sphenoid sinus and pituitary gland. Surgical intervention in the region of the sphenoid sinus pituitary gland requires extreme precision because of enveloping vital structures. The rhinologic approach to the pituitary gland, once abandoned due to limited visibility and the risk of meningitis, has experienced a renaissance as a result of recent medical and technical advances. Antibiotics have almost eliminated the risk of meningitis, and the operating microscope and televised radiofluoroscope now provide sufficient illumination, magnification, and orientation that injury to nearby structures can be avoided. A mid-line rhinologic approach provides ample exposure and further minimizes operative hazards.", "PMID": 979494}
{"id": "pubmed23n0032_12136", "title": "Auditory thresholds in the guinea pig: a preliminary report of a behavioral technique employing a food reward.", "content": "Guinea pigs have been trained to press a lever for a food reward. Then the behavior was used to obtain the auditory thresholds for six guinea pigs. The thresholds obtained in the present study agree with those previously obtained by Heffner, et al. The most important aspect of the training process was the habituation of the guinea pigs. Once the guinea pig was accustomed to the food and training cage, he could be trained in a manner similar to that used to train many other species.", "contents": "Auditory thresholds in the guinea pig: a preliminary report of a behavioral technique employing a food reward. Guinea pigs have been trained to press a lever for a food reward. Then the behavior was used to obtain the auditory thresholds for six guinea pigs. The thresholds obtained in the present study agree with those previously obtained by Heffner, et al. The most important aspect of the training process was the habituation of the guinea pigs. Once the guinea pig was accustomed to the food and training cage, he could be trained in a manner similar to that used to train many other species.", "PMID": 979495}
{"id": "pubmed23n0032_12137", "title": "An unusual application of a CROS hearing aid.", "content": "This paper describes the use of the CROS hearing aid principle on cases of congenital atresia. This young girl has had several operations which attempted to create a canal and tympanum, but the canal persisted in closing. A bone vibrator eyeglass hearing aid was fitted but was largely unsuccessful. Having a 50 db air-conduction loss was handicapping to this youngster, but the usual head worn hearing aid could not be fitted because of the lack of any canal, causing feedback. She refused a body type aid. The solution is this case was a CROS behind-the-ear hearing aid. She enjoys about a 25 db useful gain from this fitting.", "contents": "An unusual application of a CROS hearing aid. This paper describes the use of the CROS hearing aid principle on cases of congenital atresia. This young girl has had several operations which attempted to create a canal and tympanum, but the canal persisted in closing. A bone vibrator eyeglass hearing aid was fitted but was largely unsuccessful. Having a 50 db air-conduction loss was handicapping to this youngster, but the usual head worn hearing aid could not be fitted because of the lack of any canal, causing feedback. She refused a body type aid. The solution is this case was a CROS behind-the-ear hearing aid. She enjoys about a 25 db useful gain from this fitting.", "PMID": 979496}
{"id": "pubmed23n0032_12138", "title": "Nasal obstruction caused by a pituitary chromophobe adenoma.", "content": "Nasal obstruction as the only clinical manifestation of a pituitary tumor is rare. A case is reported of a 58-year-old white male with obstruction of the nose and nasopharynx due to a massive pituitary tumor, which recurred 21 years after primary radiation treatment. At autopsy, extensive destruction of the sphenoid and base of the skull was noted. Final pathologic diagnosis was Chromophobe Adenoma. Common clinical features of chromophobe adenomas are discussed.", "contents": "Nasal obstruction caused by a pituitary chromophobe adenoma. Nasal obstruction as the only clinical manifestation of a pituitary tumor is rare. A case is reported of a 58-year-old white male with obstruction of the nose and nasopharynx due to a massive pituitary tumor, which recurred 21 years after primary radiation treatment. At autopsy, extensive destruction of the sphenoid and base of the skull was noted. Final pathologic diagnosis was Chromophobe Adenoma. Common clinical features of chromophobe adenomas are discussed.", "PMID": 979497}
{"id": "pubmed23n0032_12139", "title": "Frontal  sinus disease. IV. Cellular response to experimentally-induced infection.", "content": "The pathogenesis of chronic frontal sinusitis is poorly understood. The canine frontal sinus model was developed to isolate experimentally the variables involved. We have recently determined that blockage of the naso-frontal duct does not, by itself, produce sinusitis or mucocele experimentally. The purpose of the present investigation is to examine the histological and ultrastructural responses of the sinus mucosa to artificially-induced infection. Bone wax was implanted in the canine frontal sinus via an osteoplastic flap, and subsequently removed three months later. Radiological, histological and ultrastructural analysis of the resultant infected mucosa was performed at periods up to one year. The sinus mucosa did not return to normal up to nine months following removal of the infection-inciting foreign body. Persistent changes included epithelial and submucosal thickening, polypoid degeneration, matting of the ciliary carpet, and \"bursting\" of the ciliary cell bodies. In the light of this and previous experiments, an insidious cycle of events leading to irreversible frontal sinus disease is postulated, requiring the interplay of at least three variables: the infectious insults, the response of the sinus mucosa, and the variable patency of the nasofrontal duct.", "contents": "Frontal  sinus disease. IV. Cellular response to experimentally-induced infection. The pathogenesis of chronic frontal sinusitis is poorly understood. The canine frontal sinus model was developed to isolate experimentally the variables involved. We have recently determined that blockage of the naso-frontal duct does not, by itself, produce sinusitis or mucocele experimentally. The purpose of the present investigation is to examine the histological and ultrastructural responses of the sinus mucosa to artificially-induced infection. Bone wax was implanted in the canine frontal sinus via an osteoplastic flap, and subsequently removed three months later. Radiological, histological and ultrastructural analysis of the resultant infected mucosa was performed at periods up to one year. The sinus mucosa did not return to normal up to nine months following removal of the infection-inciting foreign body. Persistent changes included epithelial and submucosal thickening, polypoid degeneration, matting of the ciliary carpet, and \"bursting\" of the ciliary cell bodies. In the light of this and previous experiments, an insidious cycle of events leading to irreversible frontal sinus disease is postulated, requiring the interplay of at least three variables: the infectious insults, the response of the sinus mucosa, and the variable patency of the nasofrontal duct.", "PMID": 979498}
{"id": "pubmed23n0032_12140", "title": "Metastasis of malignant melanoma of the nasal mucosa to the small intestine.", "content": "Malignant melanoma of the mucous membranes is rare and has a very poor prognosis. When localized in the nose, the tumor becomes apparent late in the course of the disease with nasal obstruction and epistaxis. Distant metastases are uncommon, but any organ of the body can be involved. A case of a malignant melanoma of the nasal mucosa with metastasis to the small intestine is presented, and the natural history of the metastatic disease is discussed.", "contents": "Metastasis of malignant melanoma of the nasal mucosa to the small intestine. Malignant melanoma of the mucous membranes is rare and has a very poor prognosis. When localized in the nose, the tumor becomes apparent late in the course of the disease with nasal obstruction and epistaxis. Distant metastases are uncommon, but any organ of the body can be involved. A case of a malignant melanoma of the nasal mucosa with metastasis to the small intestine is presented, and the natural history of the metastatic disease is discussed.", "PMID": 979499}
{"id": "pubmed23n0032_12141", "title": "A model for tracheal study.", "content": "While attempting to devise a valvular device to prevent aspiration in tracheo-esophageal anastomosis, we employed a model in the dog, which we feel would be very useful in the study of other tracheal problems. Essentially, a segment of trachea with its blood supply intact is isolated and turned through 90 degrees. One end of the segment is then anastomosed to the skin whereas the other end is either anastomosed to the gullet or covered with silastic, depending upon the required investigation. In both cases, after an interval of at least nine months, the segment continued to be lined by respiratory epithelium and no stenosis had occurred. Where silastic sheeting was employed to cover the end of the segment, the inner aspect of the silastic was covered with a thin layer of fibrous tissues lined by respiratory epithelium.", "contents": "A model for tracheal study. While attempting to devise a valvular device to prevent aspiration in tracheo-esophageal anastomosis, we employed a model in the dog, which we feel would be very useful in the study of other tracheal problems. Essentially, a segment of trachea with its blood supply intact is isolated and turned through 90 degrees. One end of the segment is then anastomosed to the skin whereas the other end is either anastomosed to the gullet or covered with silastic, depending upon the required investigation. In both cases, after an interval of at least nine months, the segment continued to be lined by respiratory epithelium and no stenosis had occurred. Where silastic sheeting was employed to cover the end of the segment, the inner aspect of the silastic was covered with a thin layer of fibrous tissues lined by respiratory epithelium.", "PMID": 979500}
{"id": "pubmed23n0032_12142", "title": "Implantation of multiple intracochlear electrodes for rehabilitation of total deafness: preliminary report.", "content": "Many instances of total deafness are due to destruction of the organ of Corti but with partial or complete preservation of the function of the cochlear nerve. In such cases, it is possible to restore some hearing by electrically stimulating the fibers of the cochlear nerve with the help of implanted electrodes. Preoperative testing with electric shocks applied to the round window have aroused sensations of noise in 45 cases of total bilateral deafness with a great variety of etiologies. The only negative results were in two cases of operated acoustic neuromas. Our operation places up to eight intracochlear electrodes, each with a separate fenestration opening into an electrically isolated compartment of the scala tympani. Stimulation of each electrode yields a different sound sensation of a pitch that depends on its location along the cochlea. Electric filters direct different frequency bands to the appropriate electrodes, with the necessary compression of dynamic range. In three experimental cases of unilateral deafness, pitch matches to the normal ear were made. In seven therapeutic operations on adult cases of acquired total bilateral deafness, speech recognition was usually relearned within a month or two. Improvement of voice quality was also dramatic. The intracochlear electrodes have been well tolerated for months, but the method of connection to the external equipment still presents difficulties.", "contents": "Implantation of multiple intracochlear electrodes for rehabilitation of total deafness: preliminary report. Many instances of total deafness are due to destruction of the organ of Corti but with partial or complete preservation of the function of the cochlear nerve. In such cases, it is possible to restore some hearing by electrically stimulating the fibers of the cochlear nerve with the help of implanted electrodes. Preoperative testing with electric shocks applied to the round window have aroused sensations of noise in 45 cases of total bilateral deafness with a great variety of etiologies. The only negative results were in two cases of operated acoustic neuromas. Our operation places up to eight intracochlear electrodes, each with a separate fenestration opening into an electrically isolated compartment of the scala tympani. Stimulation of each electrode yields a different sound sensation of a pitch that depends on its location along the cochlea. Electric filters direct different frequency bands to the appropriate electrodes, with the necessary compression of dynamic range. In three experimental cases of unilateral deafness, pitch matches to the normal ear were made. In seven therapeutic operations on adult cases of acquired total bilateral deafness, speech recognition was usually relearned within a month or two. Improvement of voice quality was also dramatic. The intracochlear electrodes have been well tolerated for months, but the method of connection to the external equipment still presents difficulties.", "PMID": 979501}
{"id": "pubmed23n0032_12143", "title": "Histiocytic medullary reticulosis with involvement of the nose.", "content": "A case of histiocytic medullary reticulosis in which nasal involvement was predominant is reported. The patient was a 33-year-old woman with a 14-month history of unilateral nasal stuffiness. The diagnosis was established by antemortem examination of films of bone marrow aspirates and by clinical features including fever, wasting, hepatosplenomegaly, anemia, and leukopenia. The histologic examination of autopsy specimens disclosed proliferation of histiocytes, which ingested nuclear debris and closed proliferation of histiocytes, which ingested nuclear debris and erythrocytes, in the necrotic lesion of the nose, sternal bone marrow, liver, spleen, thymus, uterus, ovali, and ileum. On reviewing literature on this subject, such a case of histiocytic medullary reticulosis which predominantly involves the nose is very rare.", "contents": "Histiocytic medullary reticulosis with involvement of the nose. A case of histiocytic medullary reticulosis in which nasal involvement was predominant is reported. The patient was a 33-year-old woman with a 14-month history of unilateral nasal stuffiness. The diagnosis was established by antemortem examination of films of bone marrow aspirates and by clinical features including fever, wasting, hepatosplenomegaly, anemia, and leukopenia. The histologic examination of autopsy specimens disclosed proliferation of histiocytes, which ingested nuclear debris and closed proliferation of histiocytes, which ingested nuclear debris and erythrocytes, in the necrotic lesion of the nose, sternal bone marrow, liver, spleen, thymus, uterus, ovali, and ileum. On reviewing literature on this subject, such a case of histiocytic medullary reticulosis which predominantly involves the nose is very rare.", "PMID": 979502}
{"id": "pubmed23n0032_12144", "title": "\"Practical suggestions on facial plastic surgery--how i do it\". The lower blepharoplasty.", "content": "The above-described operation is the musculocutaneous (\"skin-muscle\") flap procedure of blepharoplasty for the lower lid. The author is pleased to state that he has been very satisfied with this technique and that he has experienced no serious complications with it. Although the scar may be somewhat low by most standards, after several weeks it is hardly discernible (Fig. 7).", "contents": "\"Practical suggestions on facial plastic surgery--how i do it\". The lower blepharoplasty. The above-described operation is the musculocutaneous (\"skin-muscle\") flap procedure of blepharoplasty for the lower lid. The author is pleased to state that he has been very satisfied with this technique and that he has experienced no serious complications with it. Although the scar may be somewhat low by most standards, after several weeks it is hardly discernible (Fig. 7).", "PMID": 979503}
{"id": "pubmed23n0032_12145", "title": "Effects of exercise on coronary collateralization--angiographic studies of six patients in a supervised exercise program.", "content": "The existence of coronary collaterals in man and a positive correlation of their extent with the degree of coronary artery obstruction has been shown. The theory that functional collateralization associated with coronary occlusive disease is a response to local hypoxia is widely advocated. It was theorized here that coronary heart disease patients might enhance coronary collateralization through exercise-induced myocardial hypoxia. This pilot study was carried out to test the specificity of coronary arteriography for measuring changes in collateralization after a period of exercise training. Reports of follow-up examinations 7 years after program termination are also included. Six volunteer, male subjects--3 months to 3 years post-myocardial infarction--completed 10-12 months of medically-supervised exercise. All had an intensive cardiovascular work-up, serial treadmill exercise tests and coronary arteriography before and after training. All demonstrated the expected physical, physiological, metabolic, and psychological benefits. Two showed some definite, increased collateralization, however, in both of these the changes may have been a response to some extension of the occlusive disease and not exclusively an exercise effect. It was evident that minute changes in coronary collaterals are detectable by selective coronary arteriography, but that the specific effect of exercise on the development of collaterals could only be determined by a large-scale, controlled program with randomization of the multiple, uncontrollable variables among exercise and non-exercise populations.", "contents": "Effects of exercise on coronary collateralization--angiographic studies of six patients in a supervised exercise program. The existence of coronary collaterals in man and a positive correlation of their extent with the degree of coronary artery obstruction has been shown. The theory that functional collateralization associated with coronary occlusive disease is a response to local hypoxia is widely advocated. It was theorized here that coronary heart disease patients might enhance coronary collateralization through exercise-induced myocardial hypoxia. This pilot study was carried out to test the specificity of coronary arteriography for measuring changes in collateralization after a period of exercise training. Reports of follow-up examinations 7 years after program termination are also included. Six volunteer, male subjects--3 months to 3 years post-myocardial infarction--completed 10-12 months of medically-supervised exercise. All had an intensive cardiovascular work-up, serial treadmill exercise tests and coronary arteriography before and after training. All demonstrated the expected physical, physiological, metabolic, and psychological benefits. Two showed some definite, increased collateralization, however, in both of these the changes may have been a response to some extension of the occlusive disease and not exclusively an exercise effect. It was evident that minute changes in coronary collaterals are detectable by selective coronary arteriography, but that the specific effect of exercise on the development of collaterals could only be determined by a large-scale, controlled program with randomization of the multiple, uncontrollable variables among exercise and non-exercise populations.", "PMID": 979559}
{"id": "pubmed23n0032_12146", "title": "Effect of training on plasma catecholamines in post myocardial infarction patients.", "content": "Exercise induced plasma catecholamine levels, of post myocardial infarct patients, were compared before and after a 27 week training program in two groups. Heavy Intensity Exercise (H.I.E.) and Light Intensity Exercise (L.I.E.). The men (age 38-54 years) were exercised on a bicycle ergometer at constant submaximal work loads (test load) and to a symptom limited maximum work load, both before and after the training program. Forearm venous samples were obtained after 30 minutes rest and within 3 minutes of completion of the test load. After training a significant increase in both maximum work load and heart rate (P less than 0.05) was observed in the H.I.E. group. At the test load these individuals had a small (6%) decrease in heart rate. No significant changes were observed in the L.I.E. group at submaximum or maximum exercise. Prior to training, exercise resulted in a significant increase in norepinephrine (NE) with no change in epinephrine (E). Training resulted in a significant reduction (P less than 0.05) in the NE response at the test load of the H.I.E. group while no change was found in the L.I.E. group. Endurance jogging reduced the sympathetic response to moderate exercise.", "contents": "Effect of training on plasma catecholamines in post myocardial infarction patients. Exercise induced plasma catecholamine levels, of post myocardial infarct patients, were compared before and after a 27 week training program in two groups. Heavy Intensity Exercise (H.I.E.) and Light Intensity Exercise (L.I.E.). The men (age 38-54 years) were exercised on a bicycle ergometer at constant submaximal work loads (test load) and to a symptom limited maximum work load, both before and after the training program. Forearm venous samples were obtained after 30 minutes rest and within 3 minutes of completion of the test load. After training a significant increase in both maximum work load and heart rate (P less than 0.05) was observed in the H.I.E. group. At the test load these individuals had a small (6%) decrease in heart rate. No significant changes were observed in the L.I.E. group at submaximum or maximum exercise. Prior to training, exercise resulted in a significant increase in norepinephrine (NE) with no change in epinephrine (E). Training resulted in a significant reduction (P less than 0.05) in the NE response at the test load of the H.I.E. group while no change was found in the L.I.E. group. Endurance jogging reduced the sympathetic response to moderate exercise.", "PMID": 979560}
{"id": "pubmed23n0032_12147", "title": "Physiological effects of a sports rehabilitation program on cerebral palsied and post-poliomyelitic adolescents.", "content": "Thirty-four spastic cerebral palsied (CP) post-poliomyelitic (P) adolescents (mean age--17.9 years) participated in a twice-a-week mild conditioning program, lasting 12 months. Nine CP adolescents served as a control inactive group. The post-conditioning submaximal heart rates of the CP active group were significantly lower during 75 and 150 kpm/min work loads on an arm ergometer, compared with the respective values at the beginning of the program. Peak O2 uptake values, directly measured during an all-out arm pedalling test, were improved by some 8% in the CP active group. Neither CP inactive nor P active showed any significant changes attributable to conditioning in physiological variables obtained during the submaximal or maximal tests. Adiposity level of each of the three groups remained unchanged. The mechanical efficiency of all subjects was low (16.2%) and remained so following conditioning.", "contents": "Physiological effects of a sports rehabilitation program on cerebral palsied and post-poliomyelitic adolescents. Thirty-four spastic cerebral palsied (CP) post-poliomyelitic (P) adolescents (mean age--17.9 years) participated in a twice-a-week mild conditioning program, lasting 12 months. Nine CP adolescents served as a control inactive group. The post-conditioning submaximal heart rates of the CP active group were significantly lower during 75 and 150 kpm/min work loads on an arm ergometer, compared with the respective values at the beginning of the program. Peak O2 uptake values, directly measured during an all-out arm pedalling test, were improved by some 8% in the CP active group. Neither CP inactive nor P active showed any significant changes attributable to conditioning in physiological variables obtained during the submaximal or maximal tests. Adiposity level of each of the three groups remained unchanged. The mechanical efficiency of all subjects was low (16.2%) and remained so following conditioning.", "PMID": 979561}
{"id": "pubmed23n0032_12148", "title": "Specificity of training for motor skill under physical fatigue.", "content": "The purpose of the present study was to test the specificity of training principle from exercise physiology in the context of motor skill acquisition. Thirty-six college females were randomly assigned to either an experimental or a control group and both groups received 54 practice trials on a ladder climb task over four consecutive days (3,18,18 and 15 trials/day, respectively). The control group practiced under conditions of heavy interpolated fatigue on the first 3 days with day 3 representing the heavy-fatigue criterion conditions, while the experimental group did the same except for day 2 when a vowel-cancelling task was substituted for the fatigue task. On day 4 both groups practiced under the second criterion of non-fatigue conditions. Fatigue was induced by a step-up task so that a minimum HR of 180 beats/min was attained prior to each ladder trial. The results supported the specificity hypothesis that practicing a gross motor task under conditions of heavy-fatigue would facilitate performance of that task under criterion heavy-fatigue conditions. For the second criterion of non-fatigue practice, the data appeared to support the specificity principle but the rigorous test of the differences did not reach statistical significance. The overall evidence supports the position that regards heavy physical fatigue as a variable that can cause changes in the nature of certain motor skills so that the specificity of training principle becomes applicable.", "contents": "Specificity of training for motor skill under physical fatigue. The purpose of the present study was to test the specificity of training principle from exercise physiology in the context of motor skill acquisition. Thirty-six college females were randomly assigned to either an experimental or a control group and both groups received 54 practice trials on a ladder climb task over four consecutive days (3,18,18 and 15 trials/day, respectively). The control group practiced under conditions of heavy interpolated fatigue on the first 3 days with day 3 representing the heavy-fatigue criterion conditions, while the experimental group did the same except for day 2 when a vowel-cancelling task was substituted for the fatigue task. On day 4 both groups practiced under the second criterion of non-fatigue conditions. Fatigue was induced by a step-up task so that a minimum HR of 180 beats/min was attained prior to each ladder trial. The results supported the specificity hypothesis that practicing a gross motor task under conditions of heavy-fatigue would facilitate performance of that task under criterion heavy-fatigue conditions. For the second criterion of non-fatigue practice, the data appeared to support the specificity principle but the rigorous test of the differences did not reach statistical significance. The overall evidence supports the position that regards heavy physical fatigue as a variable that can cause changes in the nature of certain motor skills so that the specificity of training principle becomes applicable.", "PMID": 979562}
{"id": "pubmed23n0032_12149", "title": "The aerobic demands of rowing in two Olympic rowers.", "content": "The relationship between rowing speed and Vo2 was determined on two Olympic oarsmen while rowing the single, double and coxless pair shells. The speeds covered ranged from 120 to approximately 300 meters/min and resulted in oxygen demands from 1.2 to 6.45 1 O2/min. First order regression equations described the speed/Vo2 relationship for each shell. Rowing at racing speed in either shell required an aerobic expenditure of 5.8-6.0 1 O2/min.", "contents": "The aerobic demands of rowing in two Olympic rowers. The relationship between rowing speed and Vo2 was determined on two Olympic oarsmen while rowing the single, double and coxless pair shells. The speeds covered ranged from 120 to approximately 300 meters/min and resulted in oxygen demands from 1.2 to 6.45 1 O2/min. First order regression equations described the speed/Vo2 relationship for each shell. Rowing at racing speed in either shell required an aerobic expenditure of 5.8-6.0 1 O2/min.", "PMID": 979563}
{"id": "pubmed23n0032_12150", "title": "Summary of 58 cases of loss of consciousness during underwater swimming and diving.", "content": "It is well accepted that hyperventilation before breath hold swimming and diving makes it possible for a person to extend the time under water. Less well known is the fact that this maneuver can cause loss of consciousness due to hypoxia. This accident happens almost exclusively to males (56 cases). The most common age group was 16-20 years (range 12-33 years). All were known to be good swimmers or divers. Approximately 80% of the cases occurred in guarded pools. Thirty-five subjects survived the accident and of the twenty-three fatalities, there was only one good autopsy report. In this instance the findings were those associated with classical drowning preceded by hypoxia and hypercapnia. Breath holding experiments indicated that the times between loss of consciousness and death may be no longer than 2.5 minutes. The patterns associated with these cases suggest that those who are responsible for aquatic safety as supervisors or guards of pools could prevent most accidents by watching for young male swimmers who are practicing hyperventilation and underwater swimming in competition with themselves or with others.", "contents": "Summary of 58 cases of loss of consciousness during underwater swimming and diving. It is well accepted that hyperventilation before breath hold swimming and diving makes it possible for a person to extend the time under water. Less well known is the fact that this maneuver can cause loss of consciousness due to hypoxia. This accident happens almost exclusively to males (56 cases). The most common age group was 16-20 years (range 12-33 years). All were known to be good swimmers or divers. Approximately 80% of the cases occurred in guarded pools. Thirty-five subjects survived the accident and of the twenty-three fatalities, there was only one good autopsy report. In this instance the findings were those associated with classical drowning preceded by hypoxia and hypercapnia. Breath holding experiments indicated that the times between loss of consciousness and death may be no longer than 2.5 minutes. The patterns associated with these cases suggest that those who are responsible for aquatic safety as supervisors or guards of pools could prevent most accidents by watching for young male swimmers who are practicing hyperventilation and underwater swimming in competition with themselves or with others.", "PMID": 979564}
{"id": "pubmed23n0032_12151", "title": "Comparison of self-selected recovery methods on lactic acid removal rates.", "content": "The purpose of this study was to compare lactic acid removal rates during three modes of recovery from a standardized exercise bout. Each subject (N = 6) completed a 1 mile run (92.2 +/- 3.7% Vo2max). Thereafter, lactic acid removal rates were compared in the runners at each of three different modes of recovery: a) rest; b) a self-selected, continuous jogging pace (free-jogging); and c) completely uncontrolled recovery (free-intermittent) normally practiced by athletes. Venous blood samples were taken immediately after the mile run and every 5 min thereafter for 20 min. Data were expressed relative to the initial post-exercise blood sample (100%). Lactic acid removal was significantly faster during the free-jogging recovery than during the free-intermittent and the resting recoveries (P less than 0.001). Removal rates during the free-intermittent recovery were significantly faster than during the resting recovery (P less than 0.001). The results indicated that nearly optimal lactic acid removal rates were attained during the free-jogging recovery.", "contents": "Comparison of self-selected recovery methods on lactic acid removal rates. The purpose of this study was to compare lactic acid removal rates during three modes of recovery from a standardized exercise bout. Each subject (N = 6) completed a 1 mile run (92.2 +/- 3.7% Vo2max). Thereafter, lactic acid removal rates were compared in the runners at each of three different modes of recovery: a) rest; b) a self-selected, continuous jogging pace (free-jogging); and c) completely uncontrolled recovery (free-intermittent) normally practiced by athletes. Venous blood samples were taken immediately after the mile run and every 5 min thereafter for 20 min. Data were expressed relative to the initial post-exercise blood sample (100%). Lactic acid removal was significantly faster during the free-jogging recovery than during the free-intermittent and the resting recoveries (P less than 0.001). Removal rates during the free-intermittent recovery were significantly faster than during the resting recovery (P less than 0.001). The results indicated that nearly optimal lactic acid removal rates were attained during the free-jogging recovery.", "PMID": 979565}
{"id": "pubmed23n0032_12152", "title": "The validity of four bicycle ergometer tests.", "content": "The aim of this study was to compare the validity of four physical work and performance tests on bicycle ergometers commonly used in Sweden. The following four tests were compared: a) a work test of submaximal character designed for estimation of the maximal oxygen uptake of the individual (Vo2 max); b) a work test of maximal character designed for estimation of the highest work load an individual can sustain for 6 minutes (Wmax6min); c) the Cycling Strength and Endurance Test (CSET)-a performance test of maximal character consisting of a series of intermittently determined terminal thresholds; and d) a flexible work test of maximal character, the Individually Adapted Work Test (the IA-test): by a feedback mechanism the highest work load an individual can be expected to sustain for 4 minutes is determined. Criteria were cross-country runs (altogether 4) arranged a short time before and after the tests on the bicycle ergometer. Subjects were military conscripts, aged 17-25. When comparisons were made between the best prediction variable of each test, no significant differences were obtained. The size of the validity coefficients was .55-.70. However, the maximal work tests showed the highest validity coefficients. The investigation indicates that, when a work test is to be chosen, the problem, the practical conditions and the resources available should guide this choice.", "contents": "The validity of four bicycle ergometer tests. The aim of this study was to compare the validity of four physical work and performance tests on bicycle ergometers commonly used in Sweden. The following four tests were compared: a) a work test of submaximal character designed for estimation of the maximal oxygen uptake of the individual (Vo2 max); b) a work test of maximal character designed for estimation of the highest work load an individual can sustain for 6 minutes (Wmax6min); c) the Cycling Strength and Endurance Test (CSET)-a performance test of maximal character consisting of a series of intermittently determined terminal thresholds; and d) a flexible work test of maximal character, the Individually Adapted Work Test (the IA-test): by a feedback mechanism the highest work load an individual can be expected to sustain for 4 minutes is determined. Criteria were cross-country runs (altogether 4) arranged a short time before and after the tests on the bicycle ergometer. Subjects were military conscripts, aged 17-25. When comparisons were made between the best prediction variable of each test, no significant differences were obtained. The size of the validity coefficients was .55-.70. However, the maximal work tests showed the highest validity coefficients. The investigation indicates that, when a work test is to be chosen, the problem, the practical conditions and the resources available should guide this choice.", "PMID": 979566}
{"id": "pubmed23n0032_12153", "title": "Effects of dianabol and high-intensity sprint training on body composition of rats.", "content": "The effects on body composition and organ weights of anabolic steroid administration and of high-intensity sprint running, separately and in combination, were studied in young adult male rats. Dianabol (methandrostenolone) 1 mg/day for 8 weeks had no significant effects on muscle weight or lean body mass in either the trained or the sedentary animals. The program of sprint training resulted in a lower body weight, a lesser percentage of body fat (Runners, 8.5%, vs. sedentary, 13.5% P less than 0.01) and a greater relative lean body mass (runners, 91.5% vs. sedentry, 86.5%, P less than 0.01). We conclude from these results that (a) normal androgen levels in young, healthy male animals are sufficiently high so that the addition of a large dose of anabolic steroid does not result in stimulation of additional muscle growth, and (b) the changes in body composition induced by high-intensity, short-duration sprint training are qualitatively similar to those seen with endurance exercise training.", "contents": "Effects of dianabol and high-intensity sprint training on body composition of rats. The effects on body composition and organ weights of anabolic steroid administration and of high-intensity sprint running, separately and in combination, were studied in young adult male rats. Dianabol (methandrostenolone) 1 mg/day for 8 weeks had no significant effects on muscle weight or lean body mass in either the trained or the sedentary animals. The program of sprint training resulted in a lower body weight, a lesser percentage of body fat (Runners, 8.5%, vs. sedentary, 13.5% P less than 0.01) and a greater relative lean body mass (runners, 91.5% vs. sedentry, 86.5%, P less than 0.01). We conclude from these results that (a) normal androgen levels in young, healthy male animals are sufficiently high so that the addition of a large dose of anabolic steroid does not result in stimulation of additional muscle growth, and (b) the changes in body composition induced by high-intensity, short-duration sprint training are qualitatively similar to those seen with endurance exercise training.", "PMID": 979567}
{"id": "pubmed23n0032_12154", "title": "Factor analysis and multivariate scaling of anthropometric variables for the assessment of body composition.", "content": "This study was designed to identify the body composition factors measured by skinfold fat (S), girth (G), and diameter (D) variables and to provide multivariate scaling models that measure fat (F) and lean body weight (LBW) factors. A total of 7S, 11G, and 7D measurements were determined on 83 young women (YW) and 95 young men (YM). Body density (BD) was determined by the hydrostatic technique. All variables were factor analyzed, and F and LBW were used to validate isolated factors. The YM data showed one F factor and two LBW factors. LBW factor I measured muscle mass and bone structure; LBW factor II, bone D. The YW data isolated four factors: the same three of YM plus a second F factor specific to the pelvic girdle. Factor score models were used to develop scaling strategies to measure F and LBW factors. Full models of all 27 variables and restricted models of 14 S, G, and D variables were developed; the correlations between factor scores scaled from the full and restricted models ranged from 0.84 to 0.98. The multiple correlation between factor scores calculated from the restricted models and laboratory-determined F and LBW ranged from 0.90 to 0.94 for YM and from 0.88 to 0.89 for YW. The multivariate scaling models provide a valid field method of measuring body composition.", "contents": "Factor analysis and multivariate scaling of anthropometric variables for the assessment of body composition. This study was designed to identify the body composition factors measured by skinfold fat (S), girth (G), and diameter (D) variables and to provide multivariate scaling models that measure fat (F) and lean body weight (LBW) factors. A total of 7S, 11G, and 7D measurements were determined on 83 young women (YW) and 95 young men (YM). Body density (BD) was determined by the hydrostatic technique. All variables were factor analyzed, and F and LBW were used to validate isolated factors. The YM data showed one F factor and two LBW factors. LBW factor I measured muscle mass and bone structure; LBW factor II, bone D. The YW data isolated four factors: the same three of YM plus a second F factor specific to the pelvic girdle. Factor score models were used to develop scaling strategies to measure F and LBW factors. Full models of all 27 variables and restricted models of 14 S, G, and D variables were developed; the correlations between factor scores scaled from the full and restricted models ranged from 0.84 to 0.98. The multiple correlation between factor scores calculated from the restricted models and laboratory-determined F and LBW ranged from 0.90 to 0.94 for YM and from 0.88 to 0.89 for YW. The multivariate scaling models provide a valid field method of measuring body composition.", "PMID": 979568}
{"id": "pubmed23n0032_12155", "title": "Asymmetry in bicycle ergometer pedalling.", "content": "The effects of changes in speed and resistance setting on the bilateral symmetry of work output on the bicycle ergometer were studied. The cranks of a Monarch bicycle ergometer were instrumented with foil strain gauges and the bridge outputs were integrated on-line and analyzed by a program running in a Hewlett Packard 2115A computer. Twenty male subjects performed three thirty-second trials at each of nine speed and resistance combinations. Indices of asymmetry from 66-178 were found using kicking dominance (n = 20) and 56-135 using a strength dominance classification (n = 13). Day to day reliability of the index of asymmetry was found to be only 0.47; within day reliability was 0.87 for day one and 0.79 for day two. No significant effects for speed or resistance changes were shown on either day for the strength dominant subjects. When kicking dominance was considered main effects were encountered on both days for speed although there was no clear directional trend. The findings of these experiments have important implications for studies where measurements are made on the lower extremity during cycle ergometer exercise, and for competitive cyclists engaged in endurance competition.", "contents": "Asymmetry in bicycle ergometer pedalling. The effects of changes in speed and resistance setting on the bilateral symmetry of work output on the bicycle ergometer were studied. The cranks of a Monarch bicycle ergometer were instrumented with foil strain gauges and the bridge outputs were integrated on-line and analyzed by a program running in a Hewlett Packard 2115A computer. Twenty male subjects performed three thirty-second trials at each of nine speed and resistance combinations. Indices of asymmetry from 66-178 were found using kicking dominance (n = 20) and 56-135 using a strength dominance classification (n = 13). Day to day reliability of the index of asymmetry was found to be only 0.47; within day reliability was 0.87 for day one and 0.79 for day two. No significant effects for speed or resistance changes were shown on either day for the strength dominant subjects. When kicking dominance was considered main effects were encountered on both days for speed although there was no clear directional trend. The findings of these experiments have important implications for studies where measurements are made on the lower extremity during cycle ergometer exercise, and for competitive cyclists engaged in endurance competition.", "PMID": 979569}
{"id": "pubmed23n0032_12156", "title": "Evanescent effects of hypo- and hyperglucagonemia on blood glucose homeostasis.", "content": "Hypoglucagonemia (induced by somatostatin) and hyperglucagonemia (induced by infusion of physiologic amounts of glucagon) have only evanescent effects on blood glucose regulation. Despite on-going glucagon suppression by somatostatin, fasting hyperglycemia develops within 4-6 hr of insulin suppression, indicating that (1) basal glucagon secretion is not essential for the development of the diabetic state; and (2) insulin-deficiency (rather than altered glucagon secretion) is the dominant long-term factor determining glucose homeostasis in the diabetic. With respect to hyperglucagonemia, only a transient increase in splanchnic glucose output is observed in normal and diabetic subjects in response to physiologic increments in this hormone. The exaggerated hyperglycemic effect of glucagon observed in diabetics1 is thus a consequence of the failure to metabolize the glucose traniently released into the systemic circulation in response to the glucagon rather than a result of persistent stimulation of hepatic glucose production. These observations thus further underscore the essentiality of insulin deficiency in the diabetogenic action of glucagon.", "contents": "Evanescent effects of hypo- and hyperglucagonemia on blood glucose homeostasis. Hypoglucagonemia (induced by somatostatin) and hyperglucagonemia (induced by infusion of physiologic amounts of glucagon) have only evanescent effects on blood glucose regulation. Despite on-going glucagon suppression by somatostatin, fasting hyperglycemia develops within 4-6 hr of insulin suppression, indicating that (1) basal glucagon secretion is not essential for the development of the diabetic state; and (2) insulin-deficiency (rather than altered glucagon secretion) is the dominant long-term factor determining glucose homeostasis in the diabetic. With respect to hyperglucagonemia, only a transient increase in splanchnic glucose output is observed in normal and diabetic subjects in response to physiologic increments in this hormone. The exaggerated hyperglycemic effect of glucagon observed in diabetics1 is thus a consequence of the failure to metabolize the glucose traniently released into the systemic circulation in response to the glucagon rather than a result of persistent stimulation of hepatic glucose production. These observations thus further underscore the essentiality of insulin deficiency in the diabetogenic action of glucagon.", "PMID": 979638}
{"id": "pubmed23n0032_12157", "title": "Dominant inheritance of large molecular weight species of glucagon.", "content": "Excessive plasma levels of high molecular weight (MW) components of immunoreactive glucagon (IRG) have been found in three generations of a family in a pattern consistent with an autosomal dominant trait. Preliminary studies using gel filtration suggest the presence of two MW species of IRG greater than 3500 daltons in plasma of affected members who have normal oral glucose tolerance tests. The \"hyperglucagonemia\" in this pedigree is consonant with either a structural defect in biosynthetic precursors or an abnormality in enzyme-mediated cleavage of proglucagon.", "contents": "Dominant inheritance of large molecular weight species of glucagon. Excessive plasma levels of high molecular weight (MW) components of immunoreactive glucagon (IRG) have been found in three generations of a family in a pattern consistent with an autosomal dominant trait. Preliminary studies using gel filtration suggest the presence of two MW species of IRG greater than 3500 daltons in plasma of affected members who have normal oral glucose tolerance tests. The \"hyperglucagonemia\" in this pedigree is consonant with either a structural defect in biosynthetic precursors or an abnormality in enzyme-mediated cleavage of proglucagon.", "PMID": 979644}
{"id": "pubmed23n0032_12158", "title": "The formation of sporangiospores in Phycomyces.", "content": "The multinucleate state of the vegetative spores of Phycomyces blakesleeanus arises as a consequence of cleavage of cytoplasm containing a variable number (1-6) of pre-existing nuclei. No nuclear division or incorporation of 3H-thymidine was detected during maturation of the spores.", "contents": "The formation of sporangiospores in Phycomyces. The multinucleate state of the vegetative spores of Phycomyces blakesleeanus arises as a consequence of cleavage of cytoplasm containing a variable number (1-6) of pre-existing nuclei. No nuclear division or incorporation of 3H-thymidine was detected during maturation of the spores.", "PMID": 979660}
{"id": "pubmed23n0032_12159", "title": "Studies on transformation in Naegleria gruberi: effects of ions and bacterial suspensions.", "content": "The presence of electolytes inhibited the transformation of Naegleria gruberi from amoeba to flagellate, the molarity required varying with the salt used, namely 80 mM NaCl, 90 mM KCl, 50 mM CaCl2 or 60 mM MgCl2. Non-electrolytes also prevented this transformation at 250 mM for either sucrose or glucose, and this is known to be an osmotic effect. That the effect of ionic solutions was different was demonstrated by varying the time at which the environemnt was changed from distilled water to salt solution. Experiments with suspensions of either living or heat-killed bacteria in distilled water, together with the supernatants obtained when bacteria were removed by centrifugation, showed that the inhibition of transformation which occurred in bacterial suspensions was not due to any factors produced by the bacteria and present in solution. It appeared that this inhibition was brought about by the physical presence of the bacteria, either living or heat-killed, and some possible interpretations of this 'contact' phenomenon are discussed.", "contents": "Studies on transformation in Naegleria gruberi: effects of ions and bacterial suspensions. The presence of electolytes inhibited the transformation of Naegleria gruberi from amoeba to flagellate, the molarity required varying with the salt used, namely 80 mM NaCl, 90 mM KCl, 50 mM CaCl2 or 60 mM MgCl2. Non-electrolytes also prevented this transformation at 250 mM for either sucrose or glucose, and this is known to be an osmotic effect. That the effect of ionic solutions was different was demonstrated by varying the time at which the environemnt was changed from distilled water to salt solution. Experiments with suspensions of either living or heat-killed bacteria in distilled water, together with the supernatants obtained when bacteria were removed by centrifugation, showed that the inhibition of transformation which occurred in bacterial suspensions was not due to any factors produced by the bacteria and present in solution. It appeared that this inhibition was brought about by the physical presence of the bacteria, either living or heat-killed, and some possible interpretations of this 'contact' phenomenon are discussed.", "PMID": 979661}
{"id": "pubmed23n0032_12160", "title": "Development and fine structure of sclerotia and spores of the actinomycete Chainia olivacea.", "content": "Sclerotia and spores of Chainia olivacea were studied by transmission and scanning electron microscopy. Sclerotia formed by repeated branching of several hyphea. Branch tips were delimited by septa and increased in size, becoming filled with lipid-like inclusions. In mautre sclerotia, empty cells and intra-hyphal growth were observed. An electron-dense fibrillar material was deposited between hyphae and on the sclerotium surface. The similarities between these and the sclerotia of certain fungi are discussed. Spores were formed in a manner similar to that in Streptomyces species. Large inter-sporal pads were formed during ingrowth of the septa delimiting the spores.", "contents": "Development and fine structure of sclerotia and spores of the actinomycete Chainia olivacea. Sclerotia and spores of Chainia olivacea were studied by transmission and scanning electron microscopy. Sclerotia formed by repeated branching of several hyphea. Branch tips were delimited by septa and increased in size, becoming filled with lipid-like inclusions. In mautre sclerotia, empty cells and intra-hyphal growth were observed. An electron-dense fibrillar material was deposited between hyphae and on the sclerotium surface. The similarities between these and the sclerotia of certain fungi are discussed. Spores were formed in a manner similar to that in Streptomyces species. Large inter-sporal pads were formed during ingrowth of the septa delimiting the spores.", "PMID": 979662}
{"id": "pubmed23n0032_12161", "title": "[Growth of purple sulfur bacteria in the dark in anaerobic conditions].", "content": "Phototrophic sulphur bacteria Chromatium minutissimum 1 C, Ectothiorhodospira shaposhnikovii 1, and various strains of Thiocapsa roseopersicina grow in the dark under anaerobic conditions (hydrogen or argon) on organic media in the presence of sulphide, thiosulphate or molecular sulphur. Ect. shaposhnikovii grows also in the presence of sulphite, cysteine, methionine, glutathione, and, to a less degree, sulphates. Cultures of Ect. shaposhnikovii growing in the dark under anaerobic conditions assimilate fructose and liberate pyruvate, lactate, formiate and traces of acetate into the medium. These cultures can grow in the dark under anaerobic conditions assimilating not only fructose but also various C4-dicarboxylic acids, lactate, pyruvate, butyrate, and acetate. Thiocapsa grows on media containing malate, succinate, lactate, pyruvate, and acetate. Thiocapsa reseopersicina SL assimilates in the dark under anaerobic conditions fructose, glucose, glycerol, pyruvate, lactate, and acetate.", "contents": "[Growth of purple sulfur bacteria in the dark in anaerobic conditions]. Phototrophic sulphur bacteria Chromatium minutissimum 1 C, Ectothiorhodospira shaposhnikovii 1, and various strains of Thiocapsa roseopersicina grow in the dark under anaerobic conditions (hydrogen or argon) on organic media in the presence of sulphide, thiosulphate or molecular sulphur. Ect. shaposhnikovii grows also in the presence of sulphite, cysteine, methionine, glutathione, and, to a less degree, sulphates. Cultures of Ect. shaposhnikovii growing in the dark under anaerobic conditions assimilate fructose and liberate pyruvate, lactate, formiate and traces of acetate into the medium. These cultures can grow in the dark under anaerobic conditions assimilating not only fructose but also various C4-dicarboxylic acids, lactate, pyruvate, butyrate, and acetate. Thiocapsa grows on media containing malate, succinate, lactate, pyruvate, and acetate. Thiocapsa reseopersicina SL assimilates in the dark under anaerobic conditions fructose, glucose, glycerol, pyruvate, lactate, and acetate.", "PMID": 979675}
{"id": "pubmed23n0032_12162", "title": "[Sulfite oxidation by Thiocapsa roseopersicina].", "content": "Extracts of the cells of Thiocapsa roseopersicina, strains BBS and SL, grown in the light in anaerobic conditions and in the dark in aerobic conditions oxidize sulphite in the presence of cytochrome c and AMP or in the absence of AMP. The rate of the oxidation, especially AMP-dependent, is higher in the cells cultivated in the dark on a mineral medium. The oxidation of sulphite, both in the absence and presence of AMP, is related to the membrane fraction and is inhibited by p-CMB while azide and atebrine inhibit mainly AMP-independent sulphite oxidation. Two enzymes are presumed to be involved in sulphite oxidation by Thiocapsa roseopersicina: APS-reductase and sulphite: cytochrome c-oxidoreductase.", "contents": "[Sulfite oxidation by Thiocapsa roseopersicina]. Extracts of the cells of Thiocapsa roseopersicina, strains BBS and SL, grown in the light in anaerobic conditions and in the dark in aerobic conditions oxidize sulphite in the presence of cytochrome c and AMP or in the absence of AMP. The rate of the oxidation, especially AMP-dependent, is higher in the cells cultivated in the dark on a mineral medium. The oxidation of sulphite, both in the absence and presence of AMP, is related to the membrane fraction and is inhibited by p-CMB while azide and atebrine inhibit mainly AMP-independent sulphite oxidation. Two enzymes are presumed to be involved in sulphite oxidation by Thiocapsa roseopersicina: APS-reductase and sulphite: cytochrome c-oxidoreductase.", "PMID": 979676}
{"id": "pubmed23n0032_12163", "title": "[Chemistry of denitrification in sporogenous soil bacteria].", "content": "Soil sporeforming denitrifying bacteria, Bacillus filaris and Bacillus polymyxa, differ by their cultural-morphological and physiological characteristics, but are similar in the chemistry of dissimilating nitrate reduction. Two processes occur simultaneously: denitrification yielding gaseous nitrogen forms, and nitrate respiration upon which nitrates are reduced to ammonia. The ratio between the two depends on physico-chemical conditions of the environment. The chemistry of dissimilating nitrate reduction by sporeforming bacteria differs therefore from nitrate transformation by other denitrifying microorganisms: Pseudomonas, Micrococcus, Rhizobium, Achromobacter, which, according to the published evidence, are capable only of denitrification.", "contents": "[Chemistry of denitrification in sporogenous soil bacteria]. Soil sporeforming denitrifying bacteria, Bacillus filaris and Bacillus polymyxa, differ by their cultural-morphological and physiological characteristics, but are similar in the chemistry of dissimilating nitrate reduction. Two processes occur simultaneously: denitrification yielding gaseous nitrogen forms, and nitrate respiration upon which nitrates are reduced to ammonia. The ratio between the two depends on physico-chemical conditions of the environment. The chemistry of dissimilating nitrate reduction by sporeforming bacteria differs therefore from nitrate transformation by other denitrifying microorganisms: Pseudomonas, Micrococcus, Rhizobium, Achromobacter, which, according to the published evidence, are capable only of denitrification.", "PMID": 979677}
{"id": "pubmed23n0032_12164", "title": "[Effect of nitrogen source on growth of Arthrobacter simplex and its biosynthesis of cholinesterase].", "content": "The effect of organic and inorganic forms of nitrogen on biomass accumulation and cholinesterase synthesis was studied with Arthrobacter simplex var. cholinesterasus. The culture assimilates nitrogen of ammonium compounds better than other forms of inorganic nitrogen; the best nitrogen source for biosynthesis of cholinesterase is ammonium phosphate. Nitrogen of nitrates is not assimilated. The amount of biomass is almost twice as high on the medium with peptone, casein or casein hydrolysate as on the medium with mineral nitrogen, while the activity of cholinesterase on these nitrogen sources decreases 1.5--2.0 times. Yeast extract as a nitrogen source increases biomass accumulation by a factor of 2.5 and does not supress synthesis of cholinesterase. The concentration of the enzyme synthesized per unit biomass on the medium with yeast extract is the same as on the medium containing ammonium phosphate. The effect of amino acids and amides, i.e. beta-alanine, proline, amides of aspartic and glutamic acids, and their mixtures, is similar to the action of yeast extract: they stimulate biomass accumulation and do not inhibit synthesis of the enzyme. Other amino acids supress synthesis of cholinesterase. The amount of accumulated biomass in the presence of glutamic acid is twice as high as in the case of any other amino acid, and three times as high as on the medium containing ammonium phosphate. Similar action of glutamic acid is manifested when it is used in mixtures with other amino acids. On the medium containing glutamic acid as a sole source of nitrogen, an increase in biomass production is accompanied with a decrease in biosynthesis of the enzyme by 50%. Repression of the biosynthesis is less if glutamic acid is added in mixtures with proline, beta-alanine and asparagine.", "contents": "[Effect of nitrogen source on growth of Arthrobacter simplex and its biosynthesis of cholinesterase]. The effect of organic and inorganic forms of nitrogen on biomass accumulation and cholinesterase synthesis was studied with Arthrobacter simplex var. cholinesterasus. The culture assimilates nitrogen of ammonium compounds better than other forms of inorganic nitrogen; the best nitrogen source for biosynthesis of cholinesterase is ammonium phosphate. Nitrogen of nitrates is not assimilated. The amount of biomass is almost twice as high on the medium with peptone, casein or casein hydrolysate as on the medium with mineral nitrogen, while the activity of cholinesterase on these nitrogen sources decreases 1.5--2.0 times. Yeast extract as a nitrogen source increases biomass accumulation by a factor of 2.5 and does not supress synthesis of cholinesterase. The concentration of the enzyme synthesized per unit biomass on the medium with yeast extract is the same as on the medium containing ammonium phosphate. The effect of amino acids and amides, i.e. beta-alanine, proline, amides of aspartic and glutamic acids, and their mixtures, is similar to the action of yeast extract: they stimulate biomass accumulation and do not inhibit synthesis of the enzyme. Other amino acids supress synthesis of cholinesterase. The amount of accumulated biomass in the presence of glutamic acid is twice as high as in the case of any other amino acid, and three times as high as on the medium containing ammonium phosphate. Similar action of glutamic acid is manifested when it is used in mixtures with other amino acids. On the medium containing glutamic acid as a sole source of nitrogen, an increase in biomass production is accompanied with a decrease in biosynthesis of the enzyme by 50%. Repression of the biosynthesis is less if glutamic acid is added in mixtures with proline, beta-alanine and asparagine.", "PMID": 979679}
{"id": "pubmed23n0032_12165", "title": "[Optimization of the nutrient medium for Actinomyces spheroides--producer of proteolytic enzymes with fibrinolytic activity].", "content": "Optimization of the medium for growing Actinomyces spheroides producing proteolytic exoenzymes with the fibrinolytic activity was performed by the method of mathematical planning. The composition of the medium is as follows (%): glucose 7.1, sodium citrate, trisubstituted, 0.48; (NH4)2SO4, 0.15; MgSO4, 0.575; KH2PO4, 0.2; ZnSO4, 0.001; FeSO4, 0.002. The fibrinolytic activity of the cultural broth on this medium is three times as high as on the initial medium (7142 units). Changes in the ratio between components of the medium lead to changes in the ratio between the fibrinolytic and caseinolytic activities. Therefore, Act. spheroides, strain 1, produces exoenzymes which differ by their substrate specificity. The ratio between the fibrinolytic and caseinolytic activities of the complex on the optimized medium is twice as high as on the initial medium.", "contents": "[Optimization of the nutrient medium for Actinomyces spheroides--producer of proteolytic enzymes with fibrinolytic activity]. Optimization of the medium for growing Actinomyces spheroides producing proteolytic exoenzymes with the fibrinolytic activity was performed by the method of mathematical planning. The composition of the medium is as follows (%): glucose 7.1, sodium citrate, trisubstituted, 0.48; (NH4)2SO4, 0.15; MgSO4, 0.575; KH2PO4, 0.2; ZnSO4, 0.001; FeSO4, 0.002. The fibrinolytic activity of the cultural broth on this medium is three times as high as on the initial medium (7142 units). Changes in the ratio between components of the medium lead to changes in the ratio between the fibrinolytic and caseinolytic activities. Therefore, Act. spheroides, strain 1, produces exoenzymes which differ by their substrate specificity. The ratio between the fibrinolytic and caseinolytic activities of the complex on the optimized medium is twice as high as on the initial medium.", "PMID": 979678}
{"id": "pubmed23n0032_12166", "title": "[Synthesis of the nitrogen-fixing enzyme system in oligonitrophilic bacteria].", "content": "Oligonitrophilic bacteria were cultivated on a medium containing only 2.5--10.0 mg/litre of nitrogen compounds. They assimilated elementary nitrogen only after utilization of these nitrogen compounds during growth and formation of nitrogen-fixing enzyme system. Their cells grown on a medium containing high concentrations of bound nitrogen did not fix nitrogen during further incubation in the atmosphere of 15N; therefore, the enzymes involved in nitrogen fixation were induced. These organisms are characterized by diauxia during their growth on media containing \"start\" doses of nitrogen. Enzymes catalysing nitrogen fixation in azotobacter are also induced. But, contrary to oligonitrophilic bacteria, the azotobacter does not require nitrogen compounds in the medium in order to adapt to molecular nitrogen, and its growth curve is not of a biphasal character. These data and the evidence of other authors suggest that all nitrogen-fixing microorganisms are characterized by the induced, not constitutive, enzymes involved in reduction of molecular nitrogen.", "contents": "[Synthesis of the nitrogen-fixing enzyme system in oligonitrophilic bacteria]. Oligonitrophilic bacteria were cultivated on a medium containing only 2.5--10.0 mg/litre of nitrogen compounds. They assimilated elementary nitrogen only after utilization of these nitrogen compounds during growth and formation of nitrogen-fixing enzyme system. Their cells grown on a medium containing high concentrations of bound nitrogen did not fix nitrogen during further incubation in the atmosphere of 15N; therefore, the enzymes involved in nitrogen fixation were induced. These organisms are characterized by diauxia during their growth on media containing \"start\" doses of nitrogen. Enzymes catalysing nitrogen fixation in azotobacter are also induced. But, contrary to oligonitrophilic bacteria, the azotobacter does not require nitrogen compounds in the medium in order to adapt to molecular nitrogen, and its growth curve is not of a biphasal character. These data and the evidence of other authors suggest that all nitrogen-fixing microorganisms are characterized by the induced, not constitutive, enzymes involved in reduction of molecular nitrogen.", "PMID": 979681}
{"id": "pubmed23n0032_12167", "title": "[Production of chitinase by Actinomyces kurssanovii and its properties].", "content": "Actinomyces kurssanovii, a culture producing large amounts of chitinase and chitobiase, was cultivated on a medium of the following composition (%): demineralized crab shells, 3.0; K2HPO4, 0.5; peptone, 0.2; yeast extract, 0.1; MgSO4-H2O, 0.09. The maximum amount of the enzymes was synthesized after growth in a fermenter of the actinomycete during 48 hours. The highest activity of chitinase is manifested at pH 7.0 and depends on ionic composition of the buffer, being higher in veronal buffer than in phosphate or tris//HC1 buffers. The chitinase and chitobiase of the strain decompose completely colloid chitin and chitin in demineralized crab shells with the formation of N-acetyl-D-glucosamine.", "contents": "[Production of chitinase by Actinomyces kurssanovii and its properties]. Actinomyces kurssanovii, a culture producing large amounts of chitinase and chitobiase, was cultivated on a medium of the following composition (%): demineralized crab shells, 3.0; K2HPO4, 0.5; peptone, 0.2; yeast extract, 0.1; MgSO4-H2O, 0.09. The maximum amount of the enzymes was synthesized after growth in a fermenter of the actinomycete during 48 hours. The highest activity of chitinase is manifested at pH 7.0 and depends on ionic composition of the buffer, being higher in veronal buffer than in phosphate or tris//HC1 buffers. The chitinase and chitobiase of the strain decompose completely colloid chitin and chitin in demineralized crab shells with the formation of N-acetyl-D-glucosamine.", "PMID": 979680}
{"id": "pubmed23n0032_12168", "title": "[Effect of temperature on the synthesis of protein and individual amino acids by the thermotolerant yeast Candida tropicalis on media with hydrocarbons].", "content": "Synthesis of protein and amino acids by the thermotolerant yeast Candida tropicalis K-41 was studied at various temperatures of cultivation during growth on media with hydrocarbons in conditions of continuous cultivation. The content of protein in the cells decreased by 6% with an increase in growth temperature by 10 degrees C (from 29 to 39 degrees C) while excretion of free amino acids into the medium increased. The cultural broth of the cells grown at 39 degrees C contained up to 8 amino acids, with alanine, aspartic acid, serine and glycine prevailing. The content of amino acids which liberated into the medium decreased in the intracellular pool. However, the total amount of free amino acids in the system \"cell--medium\" remained constant at various temperatures of cultivation. Synthesis of free amino acids does not presumably limit total synthesis of protein by the yeast.", "contents": "[Effect of temperature on the synthesis of protein and individual amino acids by the thermotolerant yeast Candida tropicalis on media with hydrocarbons]. Synthesis of protein and amino acids by the thermotolerant yeast Candida tropicalis K-41 was studied at various temperatures of cultivation during growth on media with hydrocarbons in conditions of continuous cultivation. The content of protein in the cells decreased by 6% with an increase in growth temperature by 10 degrees C (from 29 to 39 degrees C) while excretion of free amino acids into the medium increased. The cultural broth of the cells grown at 39 degrees C contained up to 8 amino acids, with alanine, aspartic acid, serine and glycine prevailing. The content of amino acids which liberated into the medium decreased in the intracellular pool. However, the total amount of free amino acids in the system \"cell--medium\" remained constant at various temperatures of cultivation. Synthesis of free amino acids does not presumably limit total synthesis of protein by the yeast.", "PMID": 979682}
{"id": "pubmed23n0032_12169", "title": "[Effect of the physiological properties of yeast strains on their formation of higher alcohols and aldehydes and on the composition of nitrogen compounds in the fermented wort].", "content": "Dynamics of formation of by-products was studied with the following yeast races: Sacch. cerevisiae, strain Odesskaya-14 (baker's yeast); Sacch. vini, strain Prikumskaya 80/9 (wine yeast). The yeast cultures were found to be very similar by the rate of biomass accumulation, ethanol production, and the fractional composition of nitrogen compounds. The concentration of accumulated higher alcohols depended on the mass of yeast cells, their growth rate, and the duration of cultivation.", "contents": "[Effect of the physiological properties of yeast strains on their formation of higher alcohols and aldehydes and on the composition of nitrogen compounds in the fermented wort]. Dynamics of formation of by-products was studied with the following yeast races: Sacch. cerevisiae, strain Odesskaya-14 (baker's yeast); Sacch. vini, strain Prikumskaya 80/9 (wine yeast). The yeast cultures were found to be very similar by the rate of biomass accumulation, ethanol production, and the fractional composition of nitrogen compounds. The concentration of accumulated higher alcohols depended on the mass of yeast cells, their growth rate, and the duration of cultivation.", "PMID": 979683}
{"id": "pubmed23n0032_12170", "title": "[New brown chlorobacteria Prosthecochloris phaeoasteroidea nov. sp].", "content": "Two strains of new photosynthetic bacteria were isolated from salt meromictic lakes Mogilnoye and Faro. The bacteria are abligate phototrophic anaerobic cultures which utilize H2S as an electron donor by oxidizing it to elementary sulphur and sulphates. Sulphur is liberated outside the cell. The cultures contain bacteriochlorophyll e and carotenoids of the isorenierathene type. Photosynthetic structures are represented by chlorobium-vesicles. According to these properties, the cultures belong to the Chlorobiaceae family. The bacteria have no gas vacuoles. The cells form 10--20 apophyses-prosthecae which is typical of the Prostheocochloris genus. The new strains differ from Prosthecochloris aestuarii by the brown colour of the cell suspension, the composition of pigments, and ecology. They are classed as a new species Prostheocochloris phaeoasteroidea nov. sp. Diagnosis of the new species is presented.", "contents": "[New brown chlorobacteria Prosthecochloris phaeoasteroidea nov. sp]. Two strains of new photosynthetic bacteria were isolated from salt meromictic lakes Mogilnoye and Faro. The bacteria are abligate phototrophic anaerobic cultures which utilize H2S as an electron donor by oxidizing it to elementary sulphur and sulphates. Sulphur is liberated outside the cell. The cultures contain bacteriochlorophyll e and carotenoids of the isorenierathene type. Photosynthetic structures are represented by chlorobium-vesicles. According to these properties, the cultures belong to the Chlorobiaceae family. The bacteria have no gas vacuoles. The cells form 10--20 apophyses-prosthecae which is typical of the Prostheocochloris genus. The new strains differ from Prosthecochloris aestuarii by the brown colour of the cell suspension, the composition of pigments, and ecology. They are classed as a new species Prostheocochloris phaeoasteroidea nov. sp. Diagnosis of the new species is presented.", "PMID": 979684}
{"id": "pubmed23n0032_12171", "title": "[Age changes in cells in Streptococcus diacetilactis cultures].", "content": "The activity of metabolism changed in the cultures of Streptococcus diacetilactis, strain Bogdan, with aging. The number of viable cells decreased as well as the ability to evolve oxygen, to produce C-4 compounds, and to react to pH changes by liberating acids upon alkalifying or neutral products upon acidifying. After the culture had been grown during 72 hours, 2,3-butyleneglycol was found in the cultural broth, and the number of viable cells was low. As was revealed by electron microscopy, the cells were intact during 48 hours; they started to disintegrate by 72 hours. After 120 hours all cells were disintegrated. First, the cells produced no more mesosomes, became less electron dense; then the cell wall was decomposed and the contents of the cell poured through disruptions in the cytoplasmic membrane.", "contents": "[Age changes in cells in Streptococcus diacetilactis cultures]. The activity of metabolism changed in the cultures of Streptococcus diacetilactis, strain Bogdan, with aging. The number of viable cells decreased as well as the ability to evolve oxygen, to produce C-4 compounds, and to react to pH changes by liberating acids upon alkalifying or neutral products upon acidifying. After the culture had been grown during 72 hours, 2,3-butyleneglycol was found in the cultural broth, and the number of viable cells was low. As was revealed by electron microscopy, the cells were intact during 48 hours; they started to disintegrate by 72 hours. After 120 hours all cells were disintegrated. First, the cells produced no more mesosomes, became less electron dense; then the cell wall was decomposed and the contents of the cell poured through disruptions in the cytoplasmic membrane.", "PMID": 979685}
{"id": "pubmed23n0032_12172", "title": "[Cell wall of pigmentless variants of Staphylococcus aureus].", "content": "Physico-chemical properties and chemical composition of the cell wall of pigmentless variants B-3 and B-6 differ from those of the parent strain of Staphylococcus aureus 209-P. As to morphology, the wall is more thick and loose. The mutants differ also by the phagotype, the ability for sorption of acridine orange, and the rate of sedimentation of cell suspensions. The properties of teichoic acids and the content of peptides in peptidoglycan change in the cell wall of the mutants.", "contents": "[Cell wall of pigmentless variants of Staphylococcus aureus]. Physico-chemical properties and chemical composition of the cell wall of pigmentless variants B-3 and B-6 differ from those of the parent strain of Staphylococcus aureus 209-P. As to morphology, the wall is more thick and loose. The mutants differ also by the phagotype, the ability for sorption of acridine orange, and the rate of sedimentation of cell suspensions. The properties of teichoic acids and the content of peptides in peptidoglycan change in the cell wall of the mutants.", "PMID": 979686}
{"id": "pubmed23n0032_12173", "title": "[Comparative physiology of the replication of RNA-containing bacteriophages MS2 and Q beta].", "content": "The yield of the phage QB is always less than the yield of the phage MS2, the conditions of cultivation being similar. This is due to the fact that the yield of the phage QB from an infection centre is lower by a factor of 5--10 than the yield of the phage MS2. The yield of the phages may be increased by optimizing conditions of cultivation. The RNA-containg phages MS2 and QB were cultivated in the peptone-yeast growth medium in fermenters with the working volume of 30 and 50 litres. The yields were 2--5-10(13) particles/ml and 1--4-10(12) particles/ml for the phages MS2 and QB, respectively. The specific infectiveness of purified phage preparations was 2--4-10(12) particles/OD260 (MS2) and 2--4-10(11) particles/OD260 (QB).", "contents": "[Comparative physiology of the replication of RNA-containing bacteriophages MS2 and Q beta]. The yield of the phage QB is always less than the yield of the phage MS2, the conditions of cultivation being similar. This is due to the fact that the yield of the phage QB from an infection centre is lower by a factor of 5--10 than the yield of the phage MS2. The yield of the phages may be increased by optimizing conditions of cultivation. The RNA-containg phages MS2 and QB were cultivated in the peptone-yeast growth medium in fermenters with the working volume of 30 and 50 litres. The yields were 2--5-10(13) particles/ml and 1--4-10(12) particles/ml for the phages MS2 and QB, respectively. The specific infectiveness of purified phage preparations was 2--4-10(12) particles/OD260 (MS2) and 2--4-10(11) particles/OD260 (QB).", "PMID": 979687}
{"id": "pubmed23n0032_12174", "title": "[Ultrastructure of bacteriophages specific for Bacillus thuringiensis var. galleriae].", "content": "Fine structure of the phage specific for Bacillus thuringiensis var. galleriae was studied. The head of the phage is of the elongated hexagonal shape, 600 X 360 A. The tail is 400 X 40 A large and has a thickening in the basal part (70 X 120 A). A so-called \"collar\" structure is found at the site where the head joins the tail. This a disc-shaped plate with a diameter of 120 A and a hole in the centre; 12 fillaments are attached to it radially and terminated with tines. It is possible that the structure is necessary for adsorption of the phage on the cell.", "contents": "[Ultrastructure of bacteriophages specific for Bacillus thuringiensis var. galleriae]. Fine structure of the phage specific for Bacillus thuringiensis var. galleriae was studied. The head of the phage is of the elongated hexagonal shape, 600 X 360 A. The tail is 400 X 40 A large and has a thickening in the basal part (70 X 120 A). A so-called \"collar\" structure is found at the site where the head joins the tail. This a disc-shaped plate with a diameter of 120 A and a hole in the centre; 12 fillaments are attached to it radially and terminated with tines. It is possible that the structure is necessary for adsorption of the phage on the cell.", "PMID": 979688}
{"id": "pubmed23n0032_12175", "title": "[The treatment of giardiasis and trichomoniasis with resochin and metronidazole].", "content": "Giardia lamblia and Trichomonas intestinalis were isolated from the stools of male child of 7 with chronic diarrhea. After administration of Resochin and Metranidazole the diarrhea stopped immediately, the signs and symptoms ceased and the Parasites disappeared from the stools.", "contents": "[The treatment of giardiasis and trichomoniasis with resochin and metronidazole]. Giardia lamblia and Trichomonas intestinalis were isolated from the stools of male child of 7 with chronic diarrhea. After administration of Resochin and Metranidazole the diarrhea stopped immediately, the signs and symptoms ceased and the Parasites disappeared from the stools.", "PMID": 979695}
{"id": "pubmed23n0032_12176", "title": "[Providencia isolated from the stools of patients with gastroenteritis].", "content": "92 providencia strains were isolated from the stools of patients with gastroenteritis. All specimens were negative for other pathogenes such as salmonella, shigella, enteropathogenic E. coli and vibrios. Viral etiology was not investigated. The distribution according to years and months is shown in the table. 17 isolations made during August, seemed rather high and gave the impression of a small epidemic.", "contents": "[Providencia isolated from the stools of patients with gastroenteritis]. 92 providencia strains were isolated from the stools of patients with gastroenteritis. All specimens were negative for other pathogenes such as salmonella, shigella, enteropathogenic E. coli and vibrios. Viral etiology was not investigated. The distribution according to years and months is shown in the table. 17 isolations made during August, seemed rather high and gave the impression of a small epidemic.", "PMID": 979696}
{"id": "pubmed23n0032_12177", "title": "[Biological properties of Streptococcus bovis bacteriophages isolated from lysogenic cultures and sheep rumen].", "content": "Biological characteristics of eleven phages for Streptococcus bovis were investigated; seven phage were isolated from ovine rumen and four were virulent mutants of temperate phages of lysogenic cultures. The phages had many properties in common: similar morphology of negative colonies, the identical spectrum of lytic action, related antigens, absolute or high requirement of calcium ions, thermolability, and inactivation by the content of the rumen. Their susceptibility to the inactivating action of acetic acid, urea and temperature was however different. Chloroform and phenol may be used during purification and conservation of the phages.", "contents": "[Biological properties of Streptococcus bovis bacteriophages isolated from lysogenic cultures and sheep rumen]. Biological characteristics of eleven phages for Streptococcus bovis were investigated; seven phage were isolated from ovine rumen and four were virulent mutants of temperate phages of lysogenic cultures. The phages had many properties in common: similar morphology of negative colonies, the identical spectrum of lytic action, related antigens, absolute or high requirement of calcium ions, thermolability, and inactivation by the content of the rumen. Their susceptibility to the inactivating action of acetic acid, urea and temperature was however different. Chloroform and phenol may be used during purification and conservation of the phages.", "PMID": 979689}
{"id": "pubmed23n0032_12178", "title": "[Isolation and enumeration of bacteriophages against S. TYPHI, E. coli, and Shigella in the streams of Ankara].", "content": "Active bacteriophages were detected agains S. typhi Vi4, E. coli 36 and Shigella YGR in the streams of Ankara, during 27.3.1973 and 19.3.1974. 49 samples were collected from 3 chosen places. Bacteriophages in high concentrations were detected against S. typhi Vi4, E. coli 36 and Shigella Y6R even in 0.0001 ml amounts of these water samples. Besides bacteriophages, salmonella and shigella were detected as well. These studies showed that the streams in Ankara were highly contaminated with bacteria, and that the detergents and other chemicals used for cleaning in houses, which eventually mixed into the streams through sewage, did not have any bactericidal effect on bacteriophages or other pathogen organisms found in these streams.", "contents": "[Isolation and enumeration of bacteriophages against S. TYPHI, E. coli, and Shigella in the streams of Ankara]. Active bacteriophages were detected agains S. typhi Vi4, E. coli 36 and Shigella YGR in the streams of Ankara, during 27.3.1973 and 19.3.1974. 49 samples were collected from 3 chosen places. Bacteriophages in high concentrations were detected against S. typhi Vi4, E. coli 36 and Shigella Y6R even in 0.0001 ml amounts of these water samples. Besides bacteriophages, salmonella and shigella were detected as well. These studies showed that the streams in Ankara were highly contaminated with bacteria, and that the detergents and other chemicals used for cleaning in houses, which eventually mixed into the streams through sewage, did not have any bactericidal effect on bacteriophages or other pathogen organisms found in these streams.", "PMID": 979697}
{"id": "pubmed23n0032_12179", "title": "[Australia antigen in voluntary blood donors].", "content": "35.370 blood samples, collected from woluntary blood donors by the Blood Transfusion Services of the Turkish Red Crescent Society in one year, were tested by the systematic screening CEP Method for the \"Au\" antigen, according to the recommendations of WHO. The number and percentage of positive \"Au\" antigen cases are shown in Table 1. No significant difference could be detected when the relationship of \"Au\" antigen to months was considered. This can be seen in Table 2.", "contents": "[Australia antigen in voluntary blood donors]. 35.370 blood samples, collected from woluntary blood donors by the Blood Transfusion Services of the Turkish Red Crescent Society in one year, were tested by the systematic screening CEP Method for the \"Au\" antigen, according to the recommendations of WHO. The number and percentage of positive \"Au\" antigen cases are shown in Table 1. No significant difference could be detected when the relationship of \"Au\" antigen to months was considered. This can be seen in Table 2.", "PMID": 979698}
{"id": "pubmed23n0032_12180", "title": "[Oxygen consumption by suspensions of Bacillus anthracoides spores].", "content": "The respiration rate of spore suspensions of Bacillus anthracoides 96 was assayed by mass spectrometry employing a hermetically sealed reaction vessel constructed for this purpose. The rate of respiration was found to depend on the method of preparing suspensions, the duration of their storage at +4 degrees C, the physiological state of spores, and the action of a disinfectant containing chlorine on them.", "contents": "[Oxygen consumption by suspensions of Bacillus anthracoides spores]. The respiration rate of spore suspensions of Bacillus anthracoides 96 was assayed by mass spectrometry employing a hermetically sealed reaction vessel constructed for this purpose. The rate of respiration was found to depend on the method of preparing suspensions, the duration of their storage at +4 degrees C, the physiological state of spores, and the action of a disinfectant containing chlorine on them.", "PMID": 979692}
{"id": "pubmed23n0032_12181", "title": "[The fungal flora of Ankara air in January 1972 and 1974 and its relationship to allergic diseases].", "content": "The fungal flora of the air in Ankara was searched during first week of January 1972 and 1974. Different areas of the city were chosen for investigation. The central and outskirt areas showed different patterns of fungal flora during January 1972 and January 1974. In the central areas there was a remarkable decrease in yeast flora between the two years. However, mould flora was increased in some extent. No notable difference could be detected in outskirt areas. Penicillium and Aspergillus species were isolated most frequently. It is concluded that this flora was influenced by heavy pollution of the air in Ankara. This pollution is caused mainly by: (1) Heating systems with crude coal and oil, (2) Heavy motor traffic, (3) Geographical situation of the city, cup-shaped land with poor air draughts.", "contents": "[The fungal flora of Ankara air in January 1972 and 1974 and its relationship to allergic diseases]. The fungal flora of the air in Ankara was searched during first week of January 1972 and 1974. Different areas of the city were chosen for investigation. The central and outskirt areas showed different patterns of fungal flora during January 1972 and January 1974. In the central areas there was a remarkable decrease in yeast flora between the two years. However, mould flora was increased in some extent. No notable difference could be detected in outskirt areas. Penicillium and Aspergillus species were isolated most frequently. It is concluded that this flora was influenced by heavy pollution of the air in Ankara. This pollution is caused mainly by: (1) Heating systems with crude coal and oil, (2) Heavy motor traffic, (3) Geographical situation of the city, cup-shaped land with poor air draughts.", "PMID": 979699}
{"id": "pubmed23n0032_12182", "title": "[Dinitrochlorobenzene test in patients with chronic nephritis].", "content": "Dinitrochlorobenzene (DNCB) test was applied to 22 patients with chronic nephritis. Only 22% of the patients gave positive results. This was concluded to be due to the high NPN levels. It was demonstrated that this inhibition is transitory and the normal responses appear 12 hours after the return of the NPN to normal levels. When it was considered that some patients with high NPN levels showed normal DNCB reactivity and delayed rejection time, the chronic nephritis patiens reacted to organ transplants despite their normal NPN levels. It was suggested that it is one of the components of NPN which should be responsible for the above mentioned inhibition.", "contents": "[Dinitrochlorobenzene test in patients with chronic nephritis]. Dinitrochlorobenzene (DNCB) test was applied to 22 patients with chronic nephritis. Only 22% of the patients gave positive results. This was concluded to be due to the high NPN levels. It was demonstrated that this inhibition is transitory and the normal responses appear 12 hours after the return of the NPN to normal levels. When it was considered that some patients with high NPN levels showed normal DNCB reactivity and delayed rejection time, the chronic nephritis patiens reacted to organ transplants despite their normal NPN levels. It was suggested that it is one of the components of NPN which should be responsible for the above mentioned inhibition.", "PMID": 979700}
{"id": "pubmed23n0032_12183", "title": "[Spontaneous crystallization of antibiotic substance during submerged fermentation of Actinomyces hygroscopicus].", "content": "A rare case of spontaneous crystallization of the antibiotic substance was detected during submerged cultivation of Actinomyces hygroscopicus, strain 33x, under periodic conditions directly in the MTF 5L3 fermenter (\"Marubishi\"). Leaf-shaped plates of the antibiotic found in the cultural broth of the strain 33x were active against gram-positive and gram-negative microorganisms, yeasts, and fungi. The crystalline state of the structures was confirmed by the X-ray analysis. According to their UV spectrum (maximum at 233-234 nm in ethanol), the crystals belong to the group of nyphimycin A1-scopafungin, antibiotics produced by some cultures of Act. hygroscopicus.", "contents": "[Spontaneous crystallization of antibiotic substance during submerged fermentation of Actinomyces hygroscopicus]. A rare case of spontaneous crystallization of the antibiotic substance was detected during submerged cultivation of Actinomyces hygroscopicus, strain 33x, under periodic conditions directly in the MTF 5L3 fermenter (\"Marubishi\"). Leaf-shaped plates of the antibiotic found in the cultural broth of the strain 33x were active against gram-positive and gram-negative microorganisms, yeasts, and fungi. The crystalline state of the structures was confirmed by the X-ray analysis. According to their UV spectrum (maximum at 233-234 nm in ethanol), the crystals belong to the group of nyphimycin A1-scopafungin, antibiotics produced by some cultures of Act. hygroscopicus.", "PMID": 979694}
{"id": "pubmed23n0032_12184", "title": "[Salmonella essen serotype isolated from osteomyelitis of the right knee in a patient with sickle cell anemia].", "content": "E. essen serotype was isolated from the material collected from the right knee joint of a 11 years old white girl, sent to our hospital from Adana. The patient had sickle cell anemia plus osteomyelitis in the right knee. This is the first isolation of this serotype in Turkey.", "contents": "[Salmonella essen serotype isolated from osteomyelitis of the right knee in a patient with sickle cell anemia]. E. essen serotype was isolated from the material collected from the right knee joint of a 11 years old white girl, sent to our hospital from Adana. The patient had sickle cell anemia plus osteomyelitis in the right knee. This is the first isolation of this serotype in Turkey.", "PMID": 979701}
{"id": "pubmed23n0032_12185", "title": "[Lipid metabolism of yeasts in relation to their ecology].", "content": "Lipid metabolism of epiphyte and soil yeasts was found to be different depending on their ecology. The activity of lipase and lecithinase of epiphyte cultures is higher than that of soil cultures. The highest concentration of lipids was accumulated by soil yeasts, especially the Lipomyces genus. Lipids isolated from the yeast are hydrolysed by its lipase at a higher rate than olive oil, the common substrate for lipase. Lipid accumulation is detected during later stages of cultural growth when the activity of lipase decreases. No fatty acids with the number of carbon atoms below 14 and over 20 were found in cultures of the Candida genus. Fatty acids with a long chain (C22, C24, C26) are encountered more often in cultures belonging to the Rhodotorula genus cf. Lipomyces and Cryptococcus.", "contents": "[Lipid metabolism of yeasts in relation to their ecology]. Lipid metabolism of epiphyte and soil yeasts was found to be different depending on their ecology. The activity of lipase and lecithinase of epiphyte cultures is higher than that of soil cultures. The highest concentration of lipids was accumulated by soil yeasts, especially the Lipomyces genus. Lipids isolated from the yeast are hydrolysed by its lipase at a higher rate than olive oil, the common substrate for lipase. Lipid accumulation is detected during later stages of cultural growth when the activity of lipase decreases. No fatty acids with the number of carbon atoms below 14 and over 20 were found in cultures of the Candida genus. Fatty acids with a long chain (C22, C24, C26) are encountered more often in cultures belonging to the Rhodotorula genus cf. Lipomyces and Cryptococcus.", "PMID": 979690}
{"id": "pubmed23n0032_12186", "title": "[First isolation of Salmonella haifa in Turkey].", "content": "Authors report the first isolation of Salmonella haifa serotype in Ankara from the stool culture of a patient with acute gastroenteritis.", "contents": "[First isolation of Salmonella haifa in Turkey]. Authors report the first isolation of Salmonella haifa serotype in Ankara from the stool culture of a patient with acute gastroenteritis.", "PMID": 979702}
{"id": "pubmed23n0032_12187", "title": "[Fermentation equipment of the USSR Academy of Sciences Institute of Microbiology for continuous cultivation of microorganisms].", "content": "A fermentation apparatus has been constructed in the Institute of Microbiology of the USSR Academy of Sciences. The apparatus permits to cultivate microorganisms both under periodic and continuous conditions with automatic regulation of pH, Eh, temperature, measurement of pO2, etc.", "contents": "[Fermentation equipment of the USSR Academy of Sciences Institute of Microbiology for continuous cultivation of microorganisms]. A fermentation apparatus has been constructed in the Institute of Microbiology of the USSR Academy of Sciences. The apparatus permits to cultivate microorganisms both under periodic and continuous conditions with automatic regulation of pH, Eh, temperature, measurement of pO2, etc.", "PMID": 979691}
{"id": "pubmed23n0032_12188", "title": "[Brucellosis, tularemia and borreliosis isolated from wild animals captured in Ankara, Konya, Urfa and Nevsehir provinces in Turkey].", "content": "621 citellus, 41 Mus musculus, 35 microtus, 442 meriones, 70 Rattus rattus, 56 turtle, 89 hare, 1 hamster, 1 hedgehog and 1 sea snake, altogether 1379 wild animals were captured in Ankara, Konya, Urfa and Nev\u015fehir. Neither Brucella or Francisella tularansis could be isolated nor borrelia could be seen. 1/40-1/80 agglutination titers obtained in 3 out of 134 sera taken from citellus, in 3 out of 264 sera taken from guinea pigs which were inoculated with spleen, liver and kidney suspensions of wild animals. 1/40-1/80 agglutination titers obtained against brucella antigen in 3 out of 125 sera taken from citellus. No significant agglutination titers could be obtained in 35 sera taken from guinea pigs which were inoculated with the organ suspensions of wild animals. In blood samples of 2 citellus few trypanosoma were detected.", "contents": "[Brucellosis, tularemia and borreliosis isolated from wild animals captured in Ankara, Konya, Urfa and Nevsehir provinces in Turkey]. 621 citellus, 41 Mus musculus, 35 microtus, 442 meriones, 70 Rattus rattus, 56 turtle, 89 hare, 1 hamster, 1 hedgehog and 1 sea snake, altogether 1379 wild animals were captured in Ankara, Konya, Urfa and Nev\u015fehir. Neither Brucella or Francisella tularansis could be isolated nor borrelia could be seen. 1/40-1/80 agglutination titers obtained in 3 out of 134 sera taken from citellus, in 3 out of 264 sera taken from guinea pigs which were inoculated with spleen, liver and kidney suspensions of wild animals. 1/40-1/80 agglutination titers obtained against brucella antigen in 3 out of 125 sera taken from citellus. No significant agglutination titers could be obtained in 35 sera taken from guinea pigs which were inoculated with the organ suspensions of wild animals. In blood samples of 2 citellus few trypanosoma were detected.", "PMID": 979704}
{"id": "pubmed23n0032_12189", "title": "[Ratio of glycan to teichoic acid in Actinomyces thermovulgaris cell wall].", "content": "The molar ratio between glycan and teichoic acid was studied in the cell wall of Actinomyces thermovulgaris. A chain of teichoic acid consisting of 13 glycerophosphate units was found to correspond to 6--7 disaccharide units of glycan. The cell wall contains 31% of the glycan of the glycopeptide and 25% of teichoic acid. The content of glycan was calculated using the concentration of muramic acid in the wall. The cell wall was found to contain 1.02% of O-acetyl groups.", "contents": "[Ratio of glycan to teichoic acid in Actinomyces thermovulgaris cell wall]. The molar ratio between glycan and teichoic acid was studied in the cell wall of Actinomyces thermovulgaris. A chain of teichoic acid consisting of 13 glycerophosphate units was found to correspond to 6--7 disaccharide units of glycan. The cell wall contains 31% of the glycan of the glycopeptide and 25% of teichoic acid. The content of glycan was calculated using the concentration of muramic acid in the wall. The cell wall was found to contain 1.02% of O-acetyl groups.", "PMID": 979693}
{"id": "pubmed23n0032_12190", "title": "[Leukocyte migration inhibition test in agarose: a method for the detection of cellular immunity in humans].", "content": "Inhibition of leucocyte migration from a capillary tube is generally regarded as an in vitro corralate of cellular immunity in many species. However, significant difficulties have been encountered in the application of this test in human subjects. An alternative to this LMIT in agarose test, in which cells migrate between the agarose layer and the glass was proposed previously. In this study the said method was used in humans and applied to purified protein derivative (PPD), streptococcus group A antigen (STA) and phytohemaglutinin (PHA) (and to several other antigens, not mentioned in this report). A good correlation was obtained between skin sensitivity and antigen induced inhibition of peripheral leucocytes for PPD in 15 healthy subjects. Inhibition of cells were noted in 19 out of 20 subjects when PHA was used. Similar findings were obtained by STA. This agarose method is technically simple and requires much fewer cells (11 x 10(6) cells per antigen) than the capillary method. It also requires very small quantities of antigen (5 microliter per 11 x 10(6) cells). Our findings suggest the suitability of LMIT in agarose gel method in the detection of cell mediated immunity in man.", "contents": "[Leukocyte migration inhibition test in agarose: a method for the detection of cellular immunity in humans]. Inhibition of leucocyte migration from a capillary tube is generally regarded as an in vitro corralate of cellular immunity in many species. However, significant difficulties have been encountered in the application of this test in human subjects. An alternative to this LMIT in agarose test, in which cells migrate between the agarose layer and the glass was proposed previously. In this study the said method was used in humans and applied to purified protein derivative (PPD), streptococcus group A antigen (STA) and phytohemaglutinin (PHA) (and to several other antigens, not mentioned in this report). A good correlation was obtained between skin sensitivity and antigen induced inhibition of peripheral leucocytes for PPD in 15 healthy subjects. Inhibition of cells were noted in 19 out of 20 subjects when PHA was used. Similar findings were obtained by STA. This agarose method is technically simple and requires much fewer cells (11 x 10(6) cells per antigen) than the capillary method. It also requires very small quantities of antigen (5 microliter per 11 x 10(6) cells). Our findings suggest the suitability of LMIT in agarose gel method in the detection of cell mediated immunity in man.", "PMID": 979705}
{"id": "pubmed23n0032_12191", "title": "[Differences in the levels of serum immunoglobulins in various cancer patients].", "content": "IgG and IgM were purified from human peripheral blood sera and IgA from human colostrum. A method is established to measure the Ig classes quantitatively which is a modification of Mancini's radialimmundiffusion technique. Three Ig clases were detected from 136 cancer patients and compared with 34 healthy controls. In sarcoma there was an increase in the 3 classes of Ig in serum. These differences were also significant in patients with bone tumors. A raised level of three classes of Ig  was seen.", "contents": "[Differences in the levels of serum immunoglobulins in various cancer patients]. IgG and IgM were purified from human peripheral blood sera and IgA from human colostrum. A method is established to measure the Ig classes quantitatively which is a modification of Mancini's radialimmundiffusion technique. Three Ig clases were detected from 136 cancer patients and compared with 34 healthy controls. In sarcoma there was an increase in the 3 classes of Ig in serum. These differences were also significant in patients with bone tumors. A raised level of three classes of Ig  was seen.", "PMID": 979706}
{"id": "pubmed23n0032_12192", "title": "[Identification of mycobacteria strains isolated in our laboratory 1972-1975].", "content": "111 (3,34%) acid-fast bacteria were found by direct, and 128 (3.86%) acid-fast bacteria by homogenisation methods, in 3315 specimens sent to the tuberculosis laboratory of our institute from various clinics of G\u00fclhane Military Medical Academy, between October 11, 1972 and December 31, 1975. It was found that there was no great difference between the direct and the homogenisation methods, provided that these tests are carried out with great care. After the isolation of 128 mycobacteria from 3315 specimens by culturing in L\u00f6wenstein-Jensen medium they were typed by niacin, nitrate reduction, catalase, and peroxydase reactions. It was found that 124 out of 128 strains were M. tuberculosis var. bovis, atypical and saprophytic mycobacteria (3.125%).", "contents": "[Identification of mycobacteria strains isolated in our laboratory 1972-1975]. 111 (3,34%) acid-fast bacteria were found by direct, and 128 (3.86%) acid-fast bacteria by homogenisation methods, in 3315 specimens sent to the tuberculosis laboratory of our institute from various clinics of G\u00fclhane Military Medical Academy, between October 11, 1972 and December 31, 1975. It was found that there was no great difference between the direct and the homogenisation methods, provided that these tests are carried out with great care. After the isolation of 128 mycobacteria from 3315 specimens by culturing in L\u00f6wenstein-Jensen medium they were typed by niacin, nitrate reduction, catalase, and peroxydase reactions. It was found that 124 out of 128 strains were M. tuberculosis var. bovis, atypical and saprophytic mycobacteria (3.125%).", "PMID": 979707}
{"id": "pubmed23n0032_12193", "title": "[Animal and plant cancers produced by viruses and bacteria].", "content": "In this review the animal and plant cancers produced by viruses : (onkeorna, adeno, herpes, papova, pox, reovirus, Fig 1-6), bacteria : (Agrobacterium tumefaciens, Fig. 7), fungi : (Plasmodiophora brassicae, and nematode : (meloidgyne) are discussed.", "contents": "[Animal and plant cancers produced by viruses and bacteria]. In this review the animal and plant cancers produced by viruses : (onkeorna, adeno, herpes, papova, pox, reovirus, Fig 1-6), bacteria : (Agrobacterium tumefaciens, Fig. 7), fungi : (Plasmodiophora brassicae, and nematode : (meloidgyne) are discussed.", "PMID": 979708}
{"id": "pubmed23n0032_12194", "title": "British medical undergraduates in 1975: a student survey in 1975 compared with 1966.", "content": "Undergraduate medical education in Britain has been in a state of change since before the introduction of the National Health Service. Since then the changes in the General Medical Councils's recommendations concerning the medical curriculum (1947, 1957, 1967) have accompanied attention to curricular content, and the manpower requirements of the Health Service have drawn attention to the career preferences of students. Martin & Boddy (1962) reported a survey of medical students throughout the United Kingdom which was carried out under the auspices of the Association for the Study of Medical Education (ASME). A questionnaire was used which covered such areas as reactions to the medical school and curriculum, professional expectations and attitudes, and career preferences. Then in 1966 a similar survey was carried out by ASME and the National Foundation for Educational Research, providing data for the Royal Commission on Medical Education and published as Appendix 19 of the Todd Report (1968). The survey in 1966 used a questionnaire which investigated the background of the students, the course and students' reaction to it, and career aims, considering first preclinical and final year students only.", "contents": "British medical undergraduates in 1975: a student survey in 1975 compared with 1966. Undergraduate medical education in Britain has been in a state of change since before the introduction of the National Health Service. Since then the changes in the General Medical Councils's recommendations concerning the medical curriculum (1947, 1957, 1967) have accompanied attention to curricular content, and the manpower requirements of the Health Service have drawn attention to the career preferences of students. Martin & Boddy (1962) reported a survey of medical students throughout the United Kingdom which was carried out under the auspices of the Association for the Study of Medical Education (ASME). A questionnaire was used which covered such areas as reactions to the medical school and curriculum, professional expectations and attitudes, and career preferences. Then in 1966 a similar survey was carried out by ASME and the National Foundation for Educational Research, providing data for the Royal Commission on Medical Education and published as Appendix 19 of the Todd Report (1968). The survey in 1966 used a questionnaire which investigated the background of the students, the course and students' reaction to it, and career aims, considering first preclinical and final year students only.", "PMID": 979710}
{"id": "pubmed23n0032_12195", "title": "The careers of men graduates from the Royal Free Hospital School of Medicine, London.", "content": "The survey records the data supplied by the 290 respondents to a questionnaire sent out to the first 390 male graduates of the Royal Free Hospital School of Medicine who qualified during the 20 year period after the school became co-educational in 1948. As might have been expected, there was a preponderance of students from homes in Greater London and South East England. Whilst an equal entry of male and female students was felt desirable the achievement of this objective was slow, since selection was based on merit and the male applicants in the first few years seemed less well qualified for the medical training. During the period of the survey half of the entry was at the premedical stage and the average age for starting preclinical studies was 20-0 years--a little higher than that (18-9) recorded in the ASME survey for the 1966 entry to medical schools. A slightly higher number of Royal Free men took higher degrees and diplomas than did the women. On the number available it appeared that a higher percentage of Royal Free men took MRCP as compared with women or Birmingham graduates. The DA and DCH qualifications were more favoured by women than men. Three times as many men worked in, and twice as many lived in London as in South East England, a situation that was the reverse of that for Royal Free women. There was no evidence to show that the men preferred to do career training in London and then move out.", "contents": "The careers of men graduates from the Royal Free Hospital School of Medicine, London. The survey records the data supplied by the 290 respondents to a questionnaire sent out to the first 390 male graduates of the Royal Free Hospital School of Medicine who qualified during the 20 year period after the school became co-educational in 1948. As might have been expected, there was a preponderance of students from homes in Greater London and South East England. Whilst an equal entry of male and female students was felt desirable the achievement of this objective was slow, since selection was based on merit and the male applicants in the first few years seemed less well qualified for the medical training. During the period of the survey half of the entry was at the premedical stage and the average age for starting preclinical studies was 20-0 years--a little higher than that (18-9) recorded in the ASME survey for the 1966 entry to medical schools. A slightly higher number of Royal Free men took higher degrees and diplomas than did the women. On the number available it appeared that a higher percentage of Royal Free men took MRCP as compared with women or Birmingham graduates. The DA and DCH qualifications were more favoured by women than men. Three times as many men worked in, and twice as many lived in London as in South East England, a situation that was the reverse of that for Royal Free women. There was no evidence to show that the men preferred to do career training in London and then move out.", "PMID": 979711}
{"id": "pubmed23n0032_12196", "title": "Projected needs of health manpower in Saudi Arabia, 1974-90.", "content": "Although our projections are not completely accurate, due to inadequate data and crude methods of approximation, three conclusions are inescapable: 1. The nation should give priority to planning new schools for health administrators and rural health workers, and to generally expanding present schools for paramedicals. 2. Plans should proceed rapidly for the two new medical schools. 3. A Health Manpower plan based on census information and special studies should be developed over the next 2 years. This plan should have input from all concerned ministries. (The Ministry of Health has regarded a proposal from University Associate \"a group of Harvard, Johns Hopkins and A.U.B. professors\" for technical cooperation in health services planning.)", "contents": "Projected needs of health manpower in Saudi Arabia, 1974-90. Although our projections are not completely accurate, due to inadequate data and crude methods of approximation, three conclusions are inescapable: 1. The nation should give priority to planning new schools for health administrators and rural health workers, and to generally expanding present schools for paramedicals. 2. Plans should proceed rapidly for the two new medical schools. 3. A Health Manpower plan based on census information and special studies should be developed over the next 2 years. This plan should have input from all concerned ministries. (The Ministry of Health has regarded a proposal from University Associate \"a group of Harvard, Johns Hopkins and A.U.B. professors\" for technical cooperation in health services planning.)", "PMID": 979712}
{"id": "pubmed23n0032_12197", "title": "The organization of an integrated basic medical science curriculum at a new medical school, Nottingham.", "content": "The detailed organization of an integrated medical sciences curriculum from its initial planning to the final stage of \"automatic\" administration are described. Although some reduction in departmental autonomy results, this is outweighed by the advantages of a timetable and curriculum prepared, planned and carried out in a cooperative manner. Flexibility and scope for controlled change and experiment are retained.", "contents": "The organization of an integrated basic medical science curriculum at a new medical school, Nottingham. The detailed organization of an integrated medical sciences curriculum from its initial planning to the final stage of \"automatic\" administration are described. Although some reduction in departmental autonomy results, this is outweighed by the advantages of a timetable and curriculum prepared, planned and carried out in a cooperative manner. Flexibility and scope for controlled change and experiment are retained.", "PMID": 979713}
{"id": "pubmed23n0032_12198", "title": "A departmental approach for improving lecture skills of medical teachers.", "content": "The programme described here concentrated on improving the lecture skills of medical teaching staff in a single department. The training format for this programme included videotaping faculty lectures, providing written feedback, and formal training sessions. As a result of participating in this programme members of teaching staff felt that their lecture skills had improved and wanted additional training in other areas of education. The significance of the programme rests not only with its impact on the staff of this specific department but in its being a pilot of an approach to teacher development utilizing an exclusively self-instructional format.", "contents": "A departmental approach for improving lecture skills of medical teachers. The programme described here concentrated on improving the lecture skills of medical teaching staff in a single department. The training format for this programme included videotaping faculty lectures, providing written feedback, and formal training sessions. As a result of participating in this programme members of teaching staff felt that their lecture skills had improved and wanted additional training in other areas of education. The significance of the programme rests not only with its impact on the staff of this specific department but in its being a pilot of an approach to teacher development utilizing an exclusively self-instructional format.", "PMID": 979714}
{"id": "pubmed23n0032_12199", "title": "Attitudes towards the content of general practice teaching.", "content": "In a study of attitudes towards the content of general practice teaching, it was found that there was good agreement between fifth year medical students and their general practitioner tutors. The social aspects of illness were considered the most important by students and both groups emphasized the value of seeing patients in their homes with time for discussion of cases. These findings are discussed in the context of the development of teaching in general practice at Glasgow University.", "contents": "Attitudes towards the content of general practice teaching. In a study of attitudes towards the content of general practice teaching, it was found that there was good agreement between fifth year medical students and their general practitioner tutors. The social aspects of illness were considered the most important by students and both groups emphasized the value of seeing patients in their homes with time for discussion of cases. These findings are discussed in the context of the development of teaching in general practice at Glasgow University.", "PMID": 979715}
{"id": "pubmed23n0032_12200", "title": "Evaluation of a sex education workshop.", "content": "Thirty-two first year medical students who attended a 1 day sex education workshop and seven of their classmates who did not attend the workshop were given the Sex Knowledge and Attitude Test (SKAT) both 1 month before and 3 months after the workshop. The workshop group on retest had scores significantly higher than their pretest scores in the areas of sexual knowledge, autoeroticism and sexual myths. The scores of the no-workshop group did not change significantly on any of the parameters.Evidently, knowledge can be increased and attitudes liberalized by means of an intensive day-long workshop and such changes may persist beyong the immediate period of the workshop.", "contents": "Evaluation of a sex education workshop. Thirty-two first year medical students who attended a 1 day sex education workshop and seven of their classmates who did not attend the workshop were given the Sex Knowledge and Attitude Test (SKAT) both 1 month before and 3 months after the workshop. The workshop group on retest had scores significantly higher than their pretest scores in the areas of sexual knowledge, autoeroticism and sexual myths. The scores of the no-workshop group did not change significantly on any of the parameters.Evidently, knowledge can be increased and attitudes liberalized by means of an intensive day-long workshop and such changes may persist beyong the immediate period of the workshop.", "PMID": 979716}
{"id": "pubmed23n0032_12201", "title": "A comparison of student performances in answering essay-type and multiple-choice questions.", "content": "Assessment has been made of student performances in answering exam questions of different types. Three groups of students were tested on the same material in three different forms of examination: essay-type question, essay-type question modified by providing cues, and multiple choice question. The students performed better in multiple-choice and in cued essay questions than in uncued essay questions. An important difference between essay questions and multiple-choice questions is the provision of cues in the latter.", "contents": "A comparison of student performances in answering essay-type and multiple-choice questions. Assessment has been made of student performances in answering exam questions of different types. Three groups of students were tested on the same material in three different forms of examination: essay-type question, essay-type question modified by providing cues, and multiple choice question. The students performed better in multiple-choice and in cued essay questions than in uncued essay questions. An important difference between essay questions and multiple-choice questions is the provision of cues in the latter.", "PMID": 979717}
{"id": "pubmed23n0032_12202", "title": "Linear study of medical undergraduate performance.", "content": "The examination performance of a cohort of clinical undergraduates has been scrutinized. The cohort was notable for a high failure rate at the university preclinical examination, but this failure rate did not reappear consistently in all four final clinical subjects. The assaying examinations were not only valid for their objectives, but also measured a range of other attributes. Multiple choice question papers scores detect both quantitative and qualitative differences in knowledge between the high and low fail risk candidates. The effect of A-level grade is variable but is carried up to the final qualifying examinations. High performers tend to have good A-levels. The contribution of the preclinical course to the clinical qualifying examination was small, varied between the preclinical subjects and also between the clinical subjects and two streams of students. It is shown that lack of an adequate standard in science subjects before entry to university is associated with subsequent disadvantage in clinical training. Such disadvantage could be reduced by an introductory premedical course. The contributions made by the differing educational experiences within the cohort could be detected in many of the examinations; in particular, poor performance at the end of first clinical year pathology multiple choice questions paper examination was associated with poor performance at finals. Failure in the first part of finals indicated a likelihood of furhter failure, factors such as sex and motivation are shown to affect multiple choice question performance whilst others such as work overload do not. The high risk fail candidates can be detected 2 years before finals and might be given special educational treatment designed to improve their chance of first time success as there is no efficient way of identifying them before acceptance into the medical school.", "contents": "Linear study of medical undergraduate performance. The examination performance of a cohort of clinical undergraduates has been scrutinized. The cohort was notable for a high failure rate at the university preclinical examination, but this failure rate did not reappear consistently in all four final clinical subjects. The assaying examinations were not only valid for their objectives, but also measured a range of other attributes. Multiple choice question papers scores detect both quantitative and qualitative differences in knowledge between the high and low fail risk candidates. The effect of A-level grade is variable but is carried up to the final qualifying examinations. High performers tend to have good A-levels. The contribution of the preclinical course to the clinical qualifying examination was small, varied between the preclinical subjects and also between the clinical subjects and two streams of students. It is shown that lack of an adequate standard in science subjects before entry to university is associated with subsequent disadvantage in clinical training. Such disadvantage could be reduced by an introductory premedical course. The contributions made by the differing educational experiences within the cohort could be detected in many of the examinations; in particular, poor performance at the end of first clinical year pathology multiple choice questions paper examination was associated with poor performance at finals. Failure in the first part of finals indicated a likelihood of furhter failure, factors such as sex and motivation are shown to affect multiple choice question performance whilst others such as work overload do not. The high risk fail candidates can be detected 2 years before finals and might be given special educational treatment designed to improve their chance of first time success as there is no efficient way of identifying them before acceptance into the medical school.", "PMID": 979718}
{"id": "pubmed23n0032_12203", "title": "Medical student assessment of videotape for teaching in diagnostic radiology.", "content": "A series of six videotape recordings, prepared jointly by the Department of Radiodiagnosis and the Television Service, University of Leeds, described some aspects of the radiology of the chest, using only radiographs. A small group of final year medical students viewed these videotapes over the space of few days. Their scores for factual questions immediately afterwards were compared with their attitudes to the learning experience, expressed anonymously without knowledge of these scores. Students who scored the highest marks were most consistently critical of the videotapes; those with lower marks tended to record favourable attitudes when they performed best and unfavourable attitudes at other times. Scales set up by the students themselves to evaluate the experience produced a wide range of detailed and constructive suggestions. In general, students commented very favourably on the capacity of videotape to present fine radiographic details and we conclude that it is an appropriate medium for this purpose.", "contents": "Medical student assessment of videotape for teaching in diagnostic radiology. A series of six videotape recordings, prepared jointly by the Department of Radiodiagnosis and the Television Service, University of Leeds, described some aspects of the radiology of the chest, using only radiographs. A small group of final year medical students viewed these videotapes over the space of few days. Their scores for factual questions immediately afterwards were compared with their attitudes to the learning experience, expressed anonymously without knowledge of these scores. Students who scored the highest marks were most consistently critical of the videotapes; those with lower marks tended to record favourable attitudes when they performed best and unfavourable attitudes at other times. Scales set up by the students themselves to evaluate the experience produced a wide range of detailed and constructive suggestions. In general, students commented very favourably on the capacity of videotape to present fine radiographic details and we conclude that it is an appropriate medium for this purpose.", "PMID": 979719}
{"id": "pubmed23n0032_12204", "title": "Paediatric candidates' attitudes to computerized patient management problems in a certifying examination.", "content": "In September 1974 candidates who had taken a computerized patient managment problem examination (CPMP), as part of the certification process in paediatrics required by the Royal College of Physicians and Surgeons of Canada, completed a questionnaire designed to elicit their reactions to CPMPs. The results indicated that respondents were favourable to CPMPs, that there was little distraction caused by the equipment, logic or semantics of the problems, and that CPMPs were an acceptable examination technique. It was recommended that, in the future, CPMP examinees be allowed practice time to familiarize themselves with the equipment, that pre-examination instructions should be clarified, and that problems judged inadequate by candidates be revised.", "contents": "Paediatric candidates' attitudes to computerized patient management problems in a certifying examination. In September 1974 candidates who had taken a computerized patient managment problem examination (CPMP), as part of the certification process in paediatrics required by the Royal College of Physicians and Surgeons of Canada, completed a questionnaire designed to elicit their reactions to CPMPs. The results indicated that respondents were favourable to CPMPs, that there was little distraction caused by the equipment, logic or semantics of the problems, and that CPMPs were an acceptable examination technique. It was recommended that, in the future, CPMP examinees be allowed practice time to familiarize themselves with the equipment, that pre-examination instructions should be clarified, and that problems judged inadequate by candidates be revised.", "PMID": 979720}
{"id": "pubmed23n0032_12205", "title": "Hydatid disease at Royal Prince Alfred Hospital, 1964 to 1974.", "content": "A survey of hydatid disease has been carried out at the Royal Prince Alfred Hospital over a ten-year period from 1964 to 1974. It would appear that there has been no change in incidence in hydatid disease in the last 25 years. Fifty per cent of the hydatid cysts dealt with in this series occurred in the liver, and 30% were in the lung. Forty per cent of patients with lung hydatid disease were found to harbour hydatid cysts in the liver as well, while 24% of those with hydatid disease in the liver were found to have co-existent lung cysts. Twenty per cent of patients with pulmonary hydatid cysts presented with urgent complications, while only 7% of those with liver hydatid cysts presented in this way. Twenty-four per cent of the patients in this series were born outside Australia, most of the rest having been born and having lived in New South Wales. In this State, the distribution of hydatid disease remains the same as it was in 1925. Of the serological tests, the Casoni intradermal reaction was positive in 67% of cases, while the complement-fixation reaction was positive in only 52%. Seventy-eight per cent of these patients came to operation; 50% were found to have viable cysts at operation. Morbidity was high from chest infection, prolonged drainage from the site of the cyst and subphrenic abscess. Since few viable cysts were found in patients over the age of 60 years, there is a strong case for conservatism in the treatment of elderly patient with an asymptomatic calcified hydatid cyst. The hydatid-dependent mortality rate in this series was 1-8%. Over a mean follow-up period of five years, in 5% of these patients, there was a proven recurrence of hydatid disease requiring further operation.", "contents": "Hydatid disease at Royal Prince Alfred Hospital, 1964 to 1974. A survey of hydatid disease has been carried out at the Royal Prince Alfred Hospital over a ten-year period from 1964 to 1974. It would appear that there has been no change in incidence in hydatid disease in the last 25 years. Fifty per cent of the hydatid cysts dealt with in this series occurred in the liver, and 30% were in the lung. Forty per cent of patients with lung hydatid disease were found to harbour hydatid cysts in the liver as well, while 24% of those with hydatid disease in the liver were found to have co-existent lung cysts. Twenty per cent of patients with pulmonary hydatid cysts presented with urgent complications, while only 7% of those with liver hydatid cysts presented in this way. Twenty-four per cent of the patients in this series were born outside Australia, most of the rest having been born and having lived in New South Wales. In this State, the distribution of hydatid disease remains the same as it was in 1925. Of the serological tests, the Casoni intradermal reaction was positive in 67% of cases, while the complement-fixation reaction was positive in only 52%. Seventy-eight per cent of these patients came to operation; 50% were found to have viable cysts at operation. Morbidity was high from chest infection, prolonged drainage from the site of the cyst and subphrenic abscess. Since few viable cysts were found in patients over the age of 60 years, there is a strong case for conservatism in the treatment of elderly patient with an asymptomatic calcified hydatid cyst. The hydatid-dependent mortality rate in this series was 1-8%. Over a mean follow-up period of five years, in 5% of these patients, there was a proven recurrence of hydatid disease requiring further operation.", "PMID": 979745}
{"id": "pubmed23n0032_12206", "title": "Penetrating eye injuries associated with motor vehicle accidents.", "content": "A 2 1/2-year review of penetrating eye injuries resulting from motor vehicle accidents and treated at the Princess Alexandra Hospital, Brisbane, is presented. The series dates from January 1, 1972, when legislation requiring the compulsory wearing of seat belts was introduced. The review shows a downward trend in injuries compared with the previous 6 1/2 years. Almost all the patients failed to wear a lap-and-sash seat belt or, if one was worn, it was not secured properly.", "contents": "Penetrating eye injuries associated with motor vehicle accidents. A 2 1/2-year review of penetrating eye injuries resulting from motor vehicle accidents and treated at the Princess Alexandra Hospital, Brisbane, is presented. The series dates from January 1, 1972, when legislation requiring the compulsory wearing of seat belts was introduced. The review shows a downward trend in injuries compared with the previous 6 1/2 years. Almost all the patients failed to wear a lap-and-sash seat belt or, if one was worn, it was not secured properly.", "PMID": 979746}
{"id": "pubmed23n0032_12207", "title": "Deterioration in renal function in association with co-trimoxazole therapy.", "content": "Deterioration in renal function has been observed in four patients who were taking co-trimoxazole. Three patients had stable chronic renal failure, and in two of these there was a further permanent impairment of renal function. The remaining patient received co-trimoxazole while recovering from a short episode of acute oliguric renal failure. It is suggested that co-trimoxazole be withheld, or used with caution in patients with impaired renal function.", "contents": "Deterioration in renal function in association with co-trimoxazole therapy. Deterioration in renal function has been observed in four patients who were taking co-trimoxazole. Three patients had stable chronic renal failure, and in two of these there was a further permanent impairment of renal function. The remaining patient received co-trimoxazole while recovering from a short episode of acute oliguric renal failure. It is suggested that co-trimoxazole be withheld, or used with caution in patients with impaired renal function.", "PMID": 979747}
{"id": "pubmed23n0032_12208", "title": "Skin-prick test reactions to inhalant allergens in asthmatic patients.", "content": "Skin-prick tests with extracts of a large number of inhalant allergens were carried out on 489 asthmatic patients attending the thoracic outpatient clinics at two Sydney hospitals. Positive reactions, which had been previously found to be invariably accompanied by bronchial immediate allergic reactivity, occurred in 98% of patients who had had symptoms of asthma since the first decade of life, and in 28% of those whose symptoms did not begin until after the age of 60 years. Prick-test reactions were most commonly produced by house dust and house dust mite (70%), followed by grass pollens (50%), moulds (30%), weed pollens (35%) and tree pollens (22%). The patients commonly gave positive reactions to two or more of the extracts from within each of those allergen groups.", "contents": "Skin-prick test reactions to inhalant allergens in asthmatic patients. Skin-prick tests with extracts of a large number of inhalant allergens were carried out on 489 asthmatic patients attending the thoracic outpatient clinics at two Sydney hospitals. Positive reactions, which had been previously found to be invariably accompanied by bronchial immediate allergic reactivity, occurred in 98% of patients who had had symptoms of asthma since the first decade of life, and in 28% of those whose symptoms did not begin until after the age of 60 years. Prick-test reactions were most commonly produced by house dust and house dust mite (70%), followed by grass pollens (50%), moulds (30%), weed pollens (35%) and tree pollens (22%). The patients commonly gave positive reactions to two or more of the extracts from within each of those allergen groups.", "PMID": 979748}
{"id": "pubmed23n0032_12209", "title": "Severe obstructive jaundice due to amoebic liver abscess.", "content": "A case of amoebic liver abscess is reported in which it is clearly indicated that severe obstructive jaundice can be a predominant clinical feature of the condition. Other clinical manifestations of interest are discussed.", "contents": "Severe obstructive jaundice due to amoebic liver abscess. A case of amoebic liver abscess is reported in which it is clearly indicated that severe obstructive jaundice can be a predominant clinical feature of the condition. Other clinical manifestations of interest are discussed.", "PMID": 979749}
{"id": "pubmed23n0032_12210", "title": "Home treatment of basal cell carcinoma.", "content": "The sap of the plant Euphorbia peplus is not uncommonly used as a home treatment for warts and basal cell carcinomas. This report documents its successful use on a biopsy-proven basal cell carcinoma.", "contents": "Home treatment of basal cell carcinoma. The sap of the plant Euphorbia peplus is not uncommonly used as a home treatment for warts and basal cell carcinomas. This report documents its successful use on a biopsy-proven basal cell carcinoma.", "PMID": 979751}
{"id": "pubmed23n0032_12211", "title": "Plasma carcinoembryonic antigen in an Australian hospital population.", "content": "Plasma carcinoembryonic antigen (CEA) levels have been determined by the zirconyl phosphate gel (Z-gel) method, using materials provided by Hoffman-LaRoche Inc., on 512 samples from 425 hospital patients, and on single samples from 124 normal controls (98 blood donors and 26 healthy staff members). Of the controls, 98% had CEA levels less than 5 ng/ml. Forty-six hospital patients had CEA levels above 20 ng/ml; 45 (98%) had known present or past cancer. Nineteen patients had levels between 10 and 20 ng/ml; 11 (58%) had present or past cancer. Sixty-seven patients had levels between 5 and 10 ng/ml, and most of these had non-malignant diseases; only 34% had present or past cancer. Cigarette smoking was associated with elevated CEA levels among patients with non-neoplastic diseases, notably those with cirrhosis of the liver and chronic renal disease. There was a gradation of increasing specificity for cancer with increasing levels of CEA from 5 to over 20 ng/ml; but, on the other hand, higher levels were associated with more disseminated cancer, which would be less amenable to cure.", "contents": "Plasma carcinoembryonic antigen in an Australian hospital population. Plasma carcinoembryonic antigen (CEA) levels have been determined by the zirconyl phosphate gel (Z-gel) method, using materials provided by Hoffman-LaRoche Inc., on 512 samples from 425 hospital patients, and on single samples from 124 normal controls (98 blood donors and 26 healthy staff members). Of the controls, 98% had CEA levels less than 5 ng/ml. Forty-six hospital patients had CEA levels above 20 ng/ml; 45 (98%) had known present or past cancer. Nineteen patients had levels between 10 and 20 ng/ml; 11 (58%) had present or past cancer. Sixty-seven patients had levels between 5 and 10 ng/ml, and most of these had non-malignant diseases; only 34% had present or past cancer. Cigarette smoking was associated with elevated CEA levels among patients with non-neoplastic diseases, notably those with cirrhosis of the liver and chronic renal disease. There was a gradation of increasing specificity for cancer with increasing levels of CEA from 5 to over 20 ng/ml; but, on the other hand, higher levels were associated with more disseminated cancer, which would be less amenable to cure.", "PMID": 979758}
{"id": "pubmed23n0032_12212", "title": "Prolonged antibiotic peritoneal lavage in the management of gross generalized peritonitis.", "content": "Since there is a high mortality and morbidity with conventional therapy in generalized peritonitis, a treatment regime of prolonged continuous peritoneal lavage (two to 10 days) with the lavage solution containing kanamycin (40 mug/ml) and cephalothin (100 mug/ml) was undertaken in 14 cases of gross, well established, diffuse peritonitis. Antibiotics were also given systemically. In 11 cases, in which the causative lesion was definitively repaired, no intraperitoneal complications developed and convalescence was rapid. Three patients, in whom causative lesion was not definitively repaired at operation, subsequently died. Prolonged antibiotic peritoneal lavage appears to be beneficial for patients with gross and diffuse peritoneal soiling, after the causative lesion has been repaired.", "contents": "Prolonged antibiotic peritoneal lavage in the management of gross generalized peritonitis. Since there is a high mortality and morbidity with conventional therapy in generalized peritonitis, a treatment regime of prolonged continuous peritoneal lavage (two to 10 days) with the lavage solution containing kanamycin (40 mug/ml) and cephalothin (100 mug/ml) was undertaken in 14 cases of gross, well established, diffuse peritonitis. Antibiotics were also given systemically. In 11 cases, in which the causative lesion was definitively repaired, no intraperitoneal complications developed and convalescence was rapid. Three patients, in whom causative lesion was not definitively repaired at operation, subsequently died. Prolonged antibiotic peritoneal lavage appears to be beneficial for patients with gross and diffuse peritoneal soiling, after the causative lesion has been repaired.", "PMID": 979759}
{"id": "pubmed23n0032_12213", "title": "Cosmetic caliper for use with flail leg.", "content": "A new type of long leg brace made of polypropylene with an internal shoe foot piece seems to offer distinct advantages over the older, conventional type of calipers.", "contents": "Cosmetic caliper for use with flail leg. A new type of long leg brace made of polypropylene with an internal shoe foot piece seems to offer distinct advantages over the older, conventional type of calipers.", "PMID": 979760}
{"id": "pubmed23n0032_12214", "title": "Value of metiamide in the preoperative management of the Zollinger-El.", "content": "Two cases of Zollinger-Ellison syndrome are reported in which metiamide produced marked reduction in gastric acid secretion and control of symptoms, so that elective gastrectomy was performed without mortality or morbidity.", "contents": "Value of metiamide in the preoperative management of the Zollinger-El. Two cases of Zollinger-Ellison syndrome are reported in which metiamide produced marked reduction in gastric acid secretion and control of symptoms, so that elective gastrectomy was performed without mortality or morbidity.", "PMID": 979765}
{"id": "pubmed23n0032_12215", "title": "New fibreglass casting system in orthopaedic practice.", "content": "The composition, manufacture and application of a new fibreglass casting system for use in orthopaedic practice are described. The performance of the first 51 fibreglass casts used in routine fracture work is reviewed. The advantages (the cast is waterprof, extremely light and strong) and the disadvantages (an ultraviolet light source is required to cure the cast and the fiberglass tape is rather expensive) are evaluated.", "contents": "New fibreglass casting system in orthopaedic practice. The composition, manufacture and application of a new fibreglass casting system for use in orthopaedic practice are described. The performance of the first 51 fibreglass casts used in routine fracture work is reviewed. The advantages (the cast is waterprof, extremely light and strong) and the disadvantages (an ultraviolet light source is required to cure the cast and the fiberglass tape is rather expensive) are evaluated.", "PMID": 979766}
{"id": "pubmed23n0032_12216", "title": "Microsurgical repair of Fallopian tubes.", "content": "Of three patients who underwent microsurgical repair of Fallopian tubes after tubal sterilization, two achieved intrauterine pregnancies.", "contents": "Microsurgical repair of Fallopian tubes. Of three patients who underwent microsurgical repair of Fallopian tubes after tubal sterilization, two achieved intrauterine pregnancies.", "PMID": 979767}
{"id": "pubmed23n0032_12217", "title": "Do polyunsaturated fats predispose to malignant melonoma?", "content": "The consumption of polyunsaturated fats of patients with malignant melanoma and of a control group of patients with benign naevi has been compared in order to determine whether polyunsaturated fats predispose to the development of malignant melanoma. Patients with malignant melanoma had not consumed excessive quantities of polyunsaturated fats. Thus, there was no evidence to indicate that ingestion of polyunsaturated fats is associated with an increase in incidence of melanoma.", "contents": "Do polyunsaturated fats predispose to malignant melonoma? The consumption of polyunsaturated fats of patients with malignant melanoma and of a control group of patients with benign naevi has been compared in order to determine whether polyunsaturated fats predispose to the development of malignant melanoma. Patients with malignant melanoma had not consumed excessive quantities of polyunsaturated fats. Thus, there was no evidence to indicate that ingestion of polyunsaturated fats is associated with an increase in incidence of melanoma.", "PMID": 979775}
{"id": "pubmed23n0032_12218", "title": "The use of anabolic steroids by athletes to increase body weight and strength.", "content": "Over the past 20 years the taking of anabolic steroids by healthy athletes for the purpose of increasing body weight and strength has become very widespread. The ability of these agents to cause potentially serious side effects is discussed. In a series of 20 subjects studied over 18 months, no side effects of significance were recorded, and marked increases in strength and body weight were achieved.", "contents": "The use of anabolic steroids by athletes to increase body weight and strength. Over the past 20 years the taking of anabolic steroids by healthy athletes for the purpose of increasing body weight and strength has become very widespread. The ability of these agents to cause potentially serious side effects is discussed. In a series of 20 subjects studied over 18 months, no side effects of significance were recorded, and marked increases in strength and body weight were achieved.", "PMID": 979776}
{"id": "pubmed23n0032_12219", "title": "Surgical management in atypical pulmonary tuberculosis (MAIS complex).", "content": "Forty-four patients with Mycobacterium avium-intracellulare-scrofulaceum (MAIS) complex pulmonary infection who had a surgical procedure for management of their disease are reviewed. Relapse occurred in three cases and two patients died as a late result of their surgery. This gave a satisfactory treatment result in 89% of cases. The follow-up period was from nine months to 14 years after surgery. It is suggested that, despite the advent of modern antituberculosis drugs, resection of lung tissue is still the treatment of choic in suitable cases.", "contents": "Surgical management in atypical pulmonary tuberculosis (MAIS complex). Forty-four patients with Mycobacterium avium-intracellulare-scrofulaceum (MAIS) complex pulmonary infection who had a surgical procedure for management of their disease are reviewed. Relapse occurred in three cases and two patients died as a late result of their surgery. This gave a satisfactory treatment result in 89% of cases. The follow-up period was from nine months to 14 years after surgery. It is suggested that, despite the advent of modern antituberculosis drugs, resection of lung tissue is still the treatment of choic in suitable cases.", "PMID": 979777}
{"id": "pubmed23n0032_12220", "title": "Lower limb amputation in a general hospital: a comparative review.", "content": "This paper presents the results of a recent review of lower limb amputations carried out in a general hospital, and compares them with those of previous study of similar amputations. Particular attention is paid to the type of amputation-below-knee, through-knee or above-knee--and the associated morbidity, mortality and rehabilitation prospects. There is a need for an active approach to the problems of amputation with emphasis on preoperative preparation of the patient, the operation itself and rehabilitation follow-up in an amputation clinic.", "contents": "Lower limb amputation in a general hospital: a comparative review. This paper presents the results of a recent review of lower limb amputations carried out in a general hospital, and compares them with those of previous study of similar amputations. Particular attention is paid to the type of amputation-below-knee, through-knee or above-knee--and the associated morbidity, mortality and rehabilitation prospects. There is a need for an active approach to the problems of amputation with emphasis on preoperative preparation of the patient, the operation itself and rehabilitation follow-up in an amputation clinic.", "PMID": 979783}
{"id": "pubmed23n0032_12221", "title": "Spermatocytic seminoma.", "content": "A case of spermatocytic seminoma is described. The lesion illustrates the characteristic macroscopic and microscopic pathology of this relatively rare tumour, and is the largest recorded example. The long history of 15 years draws attention to the slow growth pattern and excellent prognosis. Chromosomal analysis, hitherto undescribed for spermatocytic seminoma, reveals moderate aneuploidy of tumour cells.", "contents": "Spermatocytic seminoma. A case of spermatocytic seminoma is described. The lesion illustrates the characteristic macroscopic and microscopic pathology of this relatively rare tumour, and is the largest recorded example. The long history of 15 years draws attention to the slow growth pattern and excellent prognosis. Chromosomal analysis, hitherto undescribed for spermatocytic seminoma, reveals moderate aneuploidy of tumour cells.", "PMID": 979784}
{"id": "pubmed23n0032_12222", "title": "Bilateral renal calculi in patients receiving methyldopa.", "content": "Two patients with bilateral kidney stones had been taking methyldopa for 8 years and 10 years respectively. Their urine contained a brown pigment which darkened and precipitated on standing. This substance was identified as methlydopa. No other cause for stone formation was found, so it is suggested that the methyldopa, because of its poor solubility, provided a nidus for stone formation.", "contents": "Bilateral renal calculi in patients receiving methyldopa. Two patients with bilateral kidney stones had been taking methyldopa for 8 years and 10 years respectively. Their urine contained a brown pigment which darkened and precipitated on standing. This substance was identified as methlydopa. No other cause for stone formation was found, so it is suggested that the methyldopa, because of its poor solubility, provided a nidus for stone formation.", "PMID": 979785}
{"id": "pubmed23n0032_12223", "title": "Thalassaemia--a preventive approach.", "content": "Thalassaemia is a significant public health problem in Australia. There is a need for improved patient education about the disease in order to prevent unnecessary anxieties. The recent advent of methods for antenatal diagnosis has given emphasis to a preventive approach. A screening and counselling service established at Queen Victoria Memorial Hospital, Melbourne, is described, and some of the problems encountered are discussed. Psychological problems are common, and these need to be managed carefully.", "contents": "Thalassaemia--a preventive approach. Thalassaemia is a significant public health problem in Australia. There is a need for improved patient education about the disease in order to prevent unnecessary anxieties. The recent advent of methods for antenatal diagnosis has given emphasis to a preventive approach. A screening and counselling service established at Queen Victoria Memorial Hospital, Melbourne, is described, and some of the problems encountered are discussed. Psychological problems are common, and these need to be managed carefully.", "PMID": 979793}
{"id": "pubmed23n0032_12224", "title": "Sequential multidrug chemotherapy and radiotherapy with possible surgery for locally advanced cancer.", "content": "A preliminary report describes the use of both a single and a combined chemotherapeutic drug regime before radiotherapy for locally advanced and radioresistant cancers. Some tumours have been rendered operable with safety by this sequence. This method is suggested to replace the current practice of following surgery with radiotherapy and finally chemotherapy. In our experience, chemotherapy before radiotherapy is assisting in the effective control of locally advanced and hitherto radioresistant cancers of many types, and in converting inoperable cancers into cancers that can be resected sometimes with safety. Also, when chemotherapeutic drugs are given before radiotherapy, damage to normal adjacent tissue is less than that seen after radiotherapy alone.", "contents": "Sequential multidrug chemotherapy and radiotherapy with possible surgery for locally advanced cancer. A preliminary report describes the use of both a single and a combined chemotherapeutic drug regime before radiotherapy for locally advanced and radioresistant cancers. Some tumours have been rendered operable with safety by this sequence. This method is suggested to replace the current practice of following surgery with radiotherapy and finally chemotherapy. In our experience, chemotherapy before radiotherapy is assisting in the effective control of locally advanced and hitherto radioresistant cancers of many types, and in converting inoperable cancers into cancers that can be resected sometimes with safety. Also, when chemotherapeutic drugs are given before radiotherapy, damage to normal adjacent tissue is less than that seen after radiotherapy alone.", "PMID": 979796}
{"id": "pubmed23n0032_12225", "title": "Detection of hepatitis B surface antigen in the saliva of patients with acute hepatitis B, and of chronic carriers.", "content": "Hepatitis B surface antigen was detected by radioimmunoassay in the saliva of 11 of 25 patients with hepatitis B, for periods of up to 23 days after the onset of illness. It was also detected in the saliva of three of five chronic carriers. The presence of this antigen in the saliva may be an important factor in non-parenteral transmission of hepatitis B.", "contents": "Detection of hepatitis B surface antigen in the saliva of patients with acute hepatitis B, and of chronic carriers. Hepatitis B surface antigen was detected by radioimmunoassay in the saliva of 11 of 25 patients with hepatitis B, for periods of up to 23 days after the onset of illness. It was also detected in the saliva of three of five chronic carriers. The presence of this antigen in the saliva may be an important factor in non-parenteral transmission of hepatitis B.", "PMID": 979797}
{"id": "pubmed23n0032_12226", "title": "Henbane chewing.", "content": "A case is presented of deliberate chewing of the flowers of henbane (Hyoscyamus niger) in the hope of producing euphoria, and an account is given of the poisoning so produced. The case indicates a more widespread use of this plant among the drug-taking community of Australia, and discussion of the case includes a description of the plant and an outline of treatment for the acute stage of the poisoning.", "contents": "Henbane chewing. A case is presented of deliberate chewing of the flowers of henbane (Hyoscyamus niger) in the hope of producing euphoria, and an account is given of the poisoning so produced. The case indicates a more widespread use of this plant among the drug-taking community of Australia, and discussion of the case includes a description of the plant and an outline of treatment for the acute stage of the poisoning.", "PMID": 979798}
{"id": "pubmed23n0032_12227", "title": "Psychotropic drug use in the elderly. Public ignorance or indifference?", "content": "Figures on prescription volume and cost for pensioners' consumption of psychotropic drugs are given, showing that their consumption is grossly overproportionate to their representation within the population. Pensioners comprise about 9% of the population and are prescribed 45% (range: 32% for minor tranquillizers to 58% for barbiturates) of all psychotropic drugs issued under the National Health Scheme. The data given are seen to be very conservative estimates of total national consumption, as they do not include prescriptions issued to repatriation and private patients, or drugs administered in hospitals. Community stereotypes about drugs and drug use are discussed. The community's knowledge of psychotropic drug use by the elderly is seen to be minimal and its attitude toward it indifferent.", "contents": "Psychotropic drug use in the elderly. Public ignorance or indifference? Figures on prescription volume and cost for pensioners' consumption of psychotropic drugs are given, showing that their consumption is grossly overproportionate to their representation within the population. Pensioners comprise about 9% of the population and are prescribed 45% (range: 32% for minor tranquillizers to 58% for barbiturates) of all psychotropic drugs issued under the National Health Scheme. The data given are seen to be very conservative estimates of total national consumption, as they do not include prescriptions issued to repatriation and private patients, or drugs administered in hospitals. Community stereotypes about drugs and drug use are discussed. The community's knowledge of psychotropic drug use by the elderly is seen to be minimal and its attitude toward it indifferent.", "PMID": 979800}
{"id": "pubmed23n0032_12228", "title": "The doctor's wife.", "content": "A sociological survey of 52 doctors' wives was performed. The majority had attended private school, 19% had reached a tertiary level of education and 58% had been employed in the health profession before marriage, 42% in the nursing profession. At the time of the survey only 10% were in full-time external employment, but the majority assisted their husbands in their professional work. A minority acknowledged some loss of personal identity in being a doctor's wife.", "contents": "The doctor's wife. A sociological survey of 52 doctors' wives was performed. The majority had attended private school, 19% had reached a tertiary level of education and 58% had been employed in the health profession before marriage, 42% in the nursing profession. At the time of the survey only 10% were in full-time external employment, but the majority assisted their husbands in their professional work. A minority acknowledged some loss of personal identity in being a doctor's wife.", "PMID": 979809}
{"id": "pubmed23n0032_12229", "title": "The Feingold dietary treatment of the hyperkinetic syndrome.", "content": "A satisfactory explanation of the hyperkinetic syndrome in children has been lacking. Feingold has advanced the hypothesis that naturally occurring salicylates and artificial food additives may cuase this syndrome in certain children , who have a genetically determined predisposition. Following Feingold's dietary prescription, an elimination diet relevant to the foods available in Sydney was developed. The treatment regime is described, and the results of its application to 15 hyperkinetic children are presented. The parents of 10 children are \"quite certain\" and those of three others \"fairly certain\" that their children's behaviour not only improved substantially with the diet, but also relapsed promptly when significant dietary infringements occurred. A possible ecological implication of these findings is briefly discussed.", "contents": "The Feingold dietary treatment of the hyperkinetic syndrome. A satisfactory explanation of the hyperkinetic syndrome in children has been lacking. Feingold has advanced the hypothesis that naturally occurring salicylates and artificial food additives may cuase this syndrome in certain children , who have a genetically determined predisposition. Following Feingold's dietary prescription, an elimination diet relevant to the foods available in Sydney was developed. The treatment regime is described, and the results of its application to 15 hyperkinetic children are presented. The parents of 10 children are \"quite certain\" and those of three others \"fairly certain\" that their children's behaviour not only improved substantially with the diet, but also relapsed promptly when significant dietary infringements occurred. A possible ecological implication of these findings is briefly discussed.", "PMID": 979817}
{"id": "pubmed23n0032_12230", "title": "Spontaneous osteonecrosis of the medial femoral condyle.", "content": "Six patients with osteonecrosis of the medial femoral condyle were seen during a 3 1/2-year period in a rheumatology unit. This condition, which may not be rare, is characterized by the sudden onset of pain in the knee of elderly people (usually women) without significant trauma. Physical signs include effusion and tenderness at the medial margin of the knee joint. Radiographic changes may not be evident for weeks or even months. The later appearances are characteristic and the site of the lesion is remarkably constant.", "contents": "Spontaneous osteonecrosis of the medial femoral condyle. Six patients with osteonecrosis of the medial femoral condyle were seen during a 3 1/2-year period in a rheumatology unit. This condition, which may not be rare, is characterized by the sudden onset of pain in the knee of elderly people (usually women) without significant trauma. Physical signs include effusion and tenderness at the medial margin of the knee joint. Radiographic changes may not be evident for weeks or even months. The later appearances are characteristic and the site of the lesion is remarkably constant.", "PMID": 979818}
{"id": "pubmed23n0032_12231", "title": "Lincomycin-clindamycin-associated psuedomembranous colitis.", "content": "Five cases of lincomycin-clindamycin-associated acute pseudomembranous colitis, demonstrating a spectrum of clinical, histological and radiological severity, were encountered over a five-months period. All patients presented with watery diarrhoea without the passage of macroscopic blood or pus. Two patients were seriously ill with fulminant colitis, but responded rapidly to corticosteroids given parenterally and supportive therapy. The diagnosis of acute colitis should be considered in all patients developing diarrhoea during or up to three weeks after beginning therapy with lincomycin or clindamycin and can be confirmed by sigmoidoscopic examination. Withdrawal of the antibiotic and symptomatic treatment is appropriate for mild cases of colitis, but our experience suggests that corticosteroid therapy is safe and effective in severe cases. Indiscriminate use of these antibiotics should be avoided.", "contents": "Lincomycin-clindamycin-associated psuedomembranous colitis. Five cases of lincomycin-clindamycin-associated acute pseudomembranous colitis, demonstrating a spectrum of clinical, histological and radiological severity, were encountered over a five-months period. All patients presented with watery diarrhoea without the passage of macroscopic blood or pus. Two patients were seriously ill with fulminant colitis, but responded rapidly to corticosteroids given parenterally and supportive therapy. The diagnosis of acute colitis should be considered in all patients developing diarrhoea during or up to three weeks after beginning therapy with lincomycin or clindamycin and can be confirmed by sigmoidoscopic examination. Withdrawal of the antibiotic and symptomatic treatment is appropriate for mild cases of colitis, but our experience suggests that corticosteroid therapy is safe and effective in severe cases. Indiscriminate use of these antibiotics should be avoided.", "PMID": 979819}
{"id": "pubmed23n0032_12232", "title": "Spleno-hepatic tuberculosis due to Mycobacterium kansasii.", "content": "A case of tuberculosis of the spleen and liver is described. The organism involved was Mycobacterium kansasii, one of the atypical mycobacteria. The lack of evidence in the literature of primary splenic or hepatic involvement by this organism suggests that it is rare. In this instance it complicated a case of myeloproliferative disease, megakaryocytic myelosis with extra-medullary haemopoiesis, and was not diagnosed until autopsy.", "contents": "Spleno-hepatic tuberculosis due to Mycobacterium kansasii. A case of tuberculosis of the spleen and liver is described. The organism involved was Mycobacterium kansasii, one of the atypical mycobacteria. The lack of evidence in the literature of primary splenic or hepatic involvement by this organism suggests that it is rare. In this instance it complicated a case of myeloproliferative disease, megakaryocytic myelosis with extra-medullary haemopoiesis, and was not diagnosed until autopsy.", "PMID": 979821}
{"id": "pubmed23n0032_12233", "title": "The use of an indwelling peritoneal catheter in the treatment of chronic renal failure.", "content": "Thirty-seven patients with end-stage renal failure were treated by dialysis by the peritoneal route, with a Tenckoff catheter. The basic regime was 30 2-litre exchanges twice a week. Two patients died while receiving peritoneal therapy, and 7 patients were transferred to haemodialysis because of catheter failure. Four patients received transplants directly from peritoneal dialysis, 22 were transferred electively to haemodialysis, and 2 are still being treated by peritoneal dialysis. Fourteen (1-2%) of the 1,161 dialyses were complicated by peritoneal infection. This was controlled in 13 instances by the addition of gentamicin to the dialysate, but removal of the catheter was required in one case. The mean duration of peritoneal dialysis was 14-4 weeks; 4 patients underwent this type of therapy for 78, 63, 41 and 40 weeks respectively.", "contents": "The use of an indwelling peritoneal catheter in the treatment of chronic renal failure. Thirty-seven patients with end-stage renal failure were treated by dialysis by the peritoneal route, with a Tenckoff catheter. The basic regime was 30 2-litre exchanges twice a week. Two patients died while receiving peritoneal therapy, and 7 patients were transferred to haemodialysis because of catheter failure. Four patients received transplants directly from peritoneal dialysis, 22 were transferred electively to haemodialysis, and 2 are still being treated by peritoneal dialysis. Fourteen (1-2%) of the 1,161 dialyses were complicated by peritoneal infection. This was controlled in 13 instances by the addition of gentamicin to the dialysate, but removal of the catheter was required in one case. The mean duration of peritoneal dialysis was 14-4 weeks; 4 patients underwent this type of therapy for 78, 63, 41 and 40 weeks respectively.", "PMID": 979823}
{"id": "pubmed23n0032_12234", "title": "Surgery for duodenal ulcer: selection of the most suitable operation for the patient.", "content": "The results of 70 consecutive operations for duodenal ulcer are presented to illustrate an approach which attempts to make the best use of the range of available operations. The object is to select patients according to criteria which minimize mortality and recurrent ulcer rate by adapting the operation to the circumstances. Functional results have been extremely satisfactory; operative mortality was 1-4% and, although the follow-up period is not sufficiently long to allow a final rate to be determined, proven recurrent ulcer rate is 2-8%. These results indicate that his approach is worth further consideration.", "contents": "Surgery for duodenal ulcer: selection of the most suitable operation for the patient. The results of 70 consecutive operations for duodenal ulcer are presented to illustrate an approach which attempts to make the best use of the range of available operations. The object is to select patients according to criteria which minimize mortality and recurrent ulcer rate by adapting the operation to the circumstances. Functional results have been extremely satisfactory; operative mortality was 1-4% and, although the follow-up period is not sufficiently long to allow a final rate to be determined, proven recurrent ulcer rate is 2-8%. These results indicate that his approach is worth further consideration.", "PMID": 979824}
{"id": "pubmed23n0032_12235", "title": "Blood alcohol and road trauma survey.", "content": "Since April 8, 1974, Victorian legislation has required that blood samples be taken from all persons aged 15 years or over after a road traffic crash. A survey was carried out of 474 such persons from June 10 until September 12, 1974. Of these, blood was taken from 350, and estimates of blood alcohol concentration were obtained from 271 samples. Sixty-five samples of the 271 (24%) gave positive results for alcohol and in about two-thirds of these the levels were higher than the legal limit of 0-05 g/100 ml. Over half of the subjects with positive results for blood alcohol concentration were under 30 years of age, 80% were males, and half were car drivers. This contrasted with the \"alcohol-negative\" subjects, who were older, and about half of whom were males. About 60% of both groups received minor injuries, and about 20% were admitted to hsopital. Research is continuing to define drinking habits and other social characteristics of these groups.", "contents": "Blood alcohol and road trauma survey. Since April 8, 1974, Victorian legislation has required that blood samples be taken from all persons aged 15 years or over after a road traffic crash. A survey was carried out of 474 such persons from June 10 until September 12, 1974. Of these, blood was taken from 350, and estimates of blood alcohol concentration were obtained from 271 samples. Sixty-five samples of the 271 (24%) gave positive results for alcohol and in about two-thirds of these the levels were higher than the legal limit of 0-05 g/100 ml. Over half of the subjects with positive results for blood alcohol concentration were under 30 years of age, 80% were males, and half were car drivers. This contrasted with the \"alcohol-negative\" subjects, who were older, and about half of whom were males. About 60% of both groups received minor injuries, and about 20% were admitted to hsopital. Research is continuing to define drinking habits and other social characteristics of these groups.", "PMID": 979825}
{"id": "pubmed23n0032_12236", "title": "Necropsy as a control of death certification: some unexpected findings.", "content": "A comparison of death certificates and necropsy findings in a group of premenopausal women suggests that a number of diseases are either underdiagnosed in life. Atypical cases of intracerebral haemorrhage are frequently misdiagnosed. These occur in the frontal, temporal or parietal lobes in non-hypertensive women and may be suitable for surgical treatment. Their aetiology remains obscure. Pulmonary embolus is habitually underdiagnosed in premenopausal women and myocardial infarcts appear to be overdiagnosed. The study reemphasizes that death certificates are inaccurate and that low necropsy rates render accurate statistics of diseases in the community difficult ot obtain.", "contents": "Necropsy as a control of death certification: some unexpected findings. A comparison of death certificates and necropsy findings in a group of premenopausal women suggests that a number of diseases are either underdiagnosed in life. Atypical cases of intracerebral haemorrhage are frequently misdiagnosed. These occur in the frontal, temporal or parietal lobes in non-hypertensive women and may be suitable for surgical treatment. Their aetiology remains obscure. Pulmonary embolus is habitually underdiagnosed in premenopausal women and myocardial infarcts appear to be overdiagnosed. The study reemphasizes that death certificates are inaccurate and that low necropsy rates render accurate statistics of diseases in the community difficult ot obtain.", "PMID": 979826}
{"id": "pubmed23n0032_12237", "title": "Unusual complication of endoscopic retrograde cholangiopancreatography.", "content": "A case is reported of extravasation of radio-opaque contrast medium into the duodenal wall surrounding the papilla of Vater during endoscopic retrograde cholangiopancreatography (ERCP). This is a particularly uncommon complication of this relatively new technique in gastrointestinal investigation. The uneventful and complete recovery in the case reported here is in line with the clinical progress of the few previously reported patients who developed this complication.", "contents": "Unusual complication of endoscopic retrograde cholangiopancreatography. A case is reported of extravasation of radio-opaque contrast medium into the duodenal wall surrounding the papilla of Vater during endoscopic retrograde cholangiopancreatography (ERCP). This is a particularly uncommon complication of this relatively new technique in gastrointestinal investigation. The uneventful and complete recovery in the case reported here is in line with the clinical progress of the few previously reported patients who developed this complication.", "PMID": 979827}
{"id": "pubmed23n0032_12238", "title": "The interaction of ethanol and delta9-tetrahydrocannabinol in man: effects on perceptual, cognitive and motor functions.", "content": "Twelve paid student volunteers (8 male, 4 female) were used in a double-blind crossover experiment to investigate the effects of delta9-tetrahydrocannabinol (THC) alone, and in combination with ethanol, on human perceptual, cognitive and motor functions. Both THC (10 mg/70 kg) and ethanol (0-5 g/kg) had little effect when administered alone. The combination of drugs, however, induced a significnat decrement in performance in some of the tests and this interaction was considered to be at least additive. The peak blood ethanol concentration was higher (P = 0-05) when subjects received both ethanol and THC than when they received ethanol alone.", "contents": "The interaction of ethanol and delta9-tetrahydrocannabinol in man: effects on perceptual, cognitive and motor functions. Twelve paid student volunteers (8 male, 4 female) were used in a double-blind crossover experiment to investigate the effects of delta9-tetrahydrocannabinol (THC) alone, and in combination with ethanol, on human perceptual, cognitive and motor functions. Both THC (10 mg/70 kg) and ethanol (0-5 g/kg) had little effect when administered alone. The combination of drugs, however, induced a significnat decrement in performance in some of the tests and this interaction was considered to be at least additive. The peak blood ethanol concentration was higher (P = 0-05) when subjects received both ethanol and THC than when they received ethanol alone.", "PMID": 979832}
{"id": "pubmed23n0032_12239", "title": "Aspiration cytology in Perth: experience of 673 cases.", "content": "The experience gained from the investigation of 673 patients by aspiration cytology is reviewed. The diagnostic accuracy of the technique is not satisfactorily demonstrated in this series owing to incomplete follow-up information. However, the almost unlimited application of aspiration cytology as a preliminary diagnostic test in focal lesions of most organs and tissues is emphasized. The chief merit of the technique is it simplicity which shoudl put it into the very front line of diagnostic procedures in suspected neoplastic disease.", "contents": "Aspiration cytology in Perth: experience of 673 cases. The experience gained from the investigation of 673 patients by aspiration cytology is reviewed. The diagnostic accuracy of the technique is not satisfactorily demonstrated in this series owing to incomplete follow-up information. However, the almost unlimited application of aspiration cytology as a preliminary diagnostic test in focal lesions of most organs and tissues is emphasized. The chief merit of the technique is it simplicity which shoudl put it into the very front line of diagnostic procedures in suspected neoplastic disease.", "PMID": 979833}
{"id": "pubmed23n0032_12240", "title": "Spironolactone and acute mountain sickness.", "content": "Thirteen adults trekking in Nepal in 1974 to altitudes between 4,300 m and 5,500 m remained free from acute mountain sickness while taking spironolactone as a prophylactic measure. Two years previously five of these adults trekking at similar altitudes, but without treatment, had suffered from acute mountain sickness. The regime used was spironolactone in a dosage of 25 mg three times a day for two days preceding and during the periods spent at altitudes above 3,000 m.", "contents": "Spironolactone and acute mountain sickness. Thirteen adults trekking in Nepal in 1974 to altitudes between 4,300 m and 5,500 m remained free from acute mountain sickness while taking spironolactone as a prophylactic measure. Two years previously five of these adults trekking at similar altitudes, but without treatment, had suffered from acute mountain sickness. The regime used was spironolactone in a dosage of 25 mg three times a day for two days preceding and during the periods spent at altitudes above 3,000 m.", "PMID": 979834}
{"id": "pubmed23n0032_12241", "title": "How many endoscopic biopsy specimens?", "content": "In cases in which the endoscopist considers it necessary to attempt to confirm or exclude a diagnosis of carcinoma of the stomach or oesophagus by biopsy and histopathological examination, we find that a minimum of four fragments should be taken. Taking more than eight biopsy specimens appears to do little more than prolong the time taken for a highly skilled team to complete the operation.", "contents": "How many endoscopic biopsy specimens? In cases in which the endoscopist considers it necessary to attempt to confirm or exclude a diagnosis of carcinoma of the stomach or oesophagus by biopsy and histopathological examination, we find that a minimum of four fragments should be taken. Taking more than eight biopsy specimens appears to do little more than prolong the time taken for a highly skilled team to complete the operation.", "PMID": 979835}
{"id": "pubmed23n0032_12242", "title": "Medullary carcinoma of the thyroid, multiple phaeochromocytomas, mucosal neuromas, marfanoid habitus and other abnormalities (Sipple's syndrome).", "content": "A case of Sipple's syndrome is reported, in which the full phenotype was expressed. The patient had the typical marfanoid habitus, with thickened lips and alae nasi, neuromas on lips and tongue, medullary carcinoma of the thyroid gland, phaeochromocytomas, and medullated corneal nerve fibres. The plasma calcitonin level was initially elevated, rose on calcium infusion before thyroidectomy, and was undetectably after thyroidectomy. The urinary catecholamine excretion was elevated. The plasma parathyroid hormone, adrenocorticotrophin and growth hormone levels and the serum calcium level were normal.", "contents": "Medullary carcinoma of the thyroid, multiple phaeochromocytomas, mucosal neuromas, marfanoid habitus and other abnormalities (Sipple's syndrome). A case of Sipple's syndrome is reported, in which the full phenotype was expressed. The patient had the typical marfanoid habitus, with thickened lips and alae nasi, neuromas on lips and tongue, medullary carcinoma of the thyroid gland, phaeochromocytomas, and medullated corneal nerve fibres. The plasma calcitonin level was initially elevated, rose on calcium infusion before thyroidectomy, and was undetectably after thyroidectomy. The urinary catecholamine excretion was elevated. The plasma parathyroid hormone, adrenocorticotrophin and growth hormone levels and the serum calcium level were normal.", "PMID": 979836}
{"id": "pubmed23n0032_12243", "title": "The evaluation of clinical competence.", "content": "This review examined new approaches to the definition and evaluation of clinical competence. Competence may be defined in terms of the components of the process of clinical performance or in terms of the outcomes of clinical performance. No single test method is capable of providing a valid and reliable evaluation of all defined aspects of clinical competence. Both in this country and overseas, new test methods and examinations are being developed and the implications which they will have for Australian medical schools are discussed.", "contents": "The evaluation of clinical competence. This review examined new approaches to the definition and evaluation of clinical competence. Competence may be defined in terms of the components of the process of clinical performance or in terms of the outcomes of clinical performance. No single test method is capable of providing a valid and reliable evaluation of all defined aspects of clinical competence. Both in this country and overseas, new test methods and examinations are being developed and the implications which they will have for Australian medical schools are discussed.", "PMID": 979837}
{"id": "pubmed23n0032_12244", "title": "Rheumatoid arthritis treated with chlorambucil: a five-year follow-up.", "content": "The results of a five-year study of chlorambucil in patients suffering from rheumatoid arthritis are presented. The effect of treatment was to allow a reduction in corticosteroid dosage and a sustained remission of disease activity in patients. Three patients of the series of 22 developed neoplastic disease, and the signficance of this is discussed.", "contents": "Rheumatoid arthritis treated with chlorambucil: a five-year follow-up. The results of a five-year study of chlorambucil in patients suffering from rheumatoid arthritis are presented. The effect of treatment was to allow a reduction in corticosteroid dosage and a sustained remission of disease activity in patients. Three patients of the series of 22 developed neoplastic disease, and the signficance of this is discussed.", "PMID": 979846}
{"id": "pubmed23n0032_12245", "title": "Prevalence of cardiovascular risk factors including fasting serum triglyceride levels in the Cunderdin health survey of 1971.", "content": "In a mass health examination conducted in the Shires of Cunderdin and Tammin, 83% of subjects on the electoral rolls attended for screening of cardiovascular risk factors including fasting blood lipid measurements. Fasting serum triglyceride levels are presented. Other factors measured showed quite close similarities to those in the Busselton 1972 Health Survey. On conventional criteria of percentage of desirable weight, fasting serum cholesterol level and fasting serum triglyceride level, a very large proportion of the survey population was found to be eligible for preventive dietary advice--for example, 50% of males and females aged 40 to 59 years. The effective education and after-care of subjects found to be at risk requires further development of resources.", "contents": "Prevalence of cardiovascular risk factors including fasting serum triglyceride levels in the Cunderdin health survey of 1971. In a mass health examination conducted in the Shires of Cunderdin and Tammin, 83% of subjects on the electoral rolls attended for screening of cardiovascular risk factors including fasting blood lipid measurements. Fasting serum triglyceride levels are presented. Other factors measured showed quite close similarities to those in the Busselton 1972 Health Survey. On conventional criteria of percentage of desirable weight, fasting serum cholesterol level and fasting serum triglyceride level, a very large proportion of the survey population was found to be eligible for preventive dietary advice--for example, 50% of males and females aged 40 to 59 years. The effective education and after-care of subjects found to be at risk requires further development of resources.", "PMID": 979847}
{"id": "pubmed23n0032_12246", "title": "Domiciliary follow-up of the patient with acute myocardial infarction and myocardial ischaemia.", "content": "For 134 patients suffering acute myocardial infarction and/or ischaemic heart disease, a cardiac programme was commenced during hospitalization and followed up in the home environment. The workings of the programme are discussed and conclusions as to its effectiveness are drawn.", "contents": "Domiciliary follow-up of the patient with acute myocardial infarction and myocardial ischaemia. For 134 patients suffering acute myocardial infarction and/or ischaemic heart disease, a cardiac programme was commenced during hospitalization and followed up in the home environment. The workings of the programme are discussed and conclusions as to its effectiveness are drawn.", "PMID": 979848}
{"id": "pubmed23n0032_12247", "title": "Gastric ulceration within hiatus hernia.", "content": "A retrospective analysis has been made of 19 persons with gastric ulceration in hiatus hernias. Eleven patients presented with acute upper gastrointestinal haemorrhage and three with chronic anaemia. Seventeen had experienced previous abdominal discomfort or heartburn. Diagnosis of ulceration was achieved by endoscopy in 16 instances and by radiography in six instances. A chronic ulcer was found in 10 patients, a discrete acute ulcer in three and multiple erosions in six. Twelve of the hernias were sliding in type and irreducible, in contrast to previous reports that gastric ulceration is most frequent in paracoesophageal hernias. Conservative management resulted in complete healing of the ulcer in the majority of patients. Continued bleeding or failure of the ulcer to heal necessitated surgical intervention in six patients.", "contents": "Gastric ulceration within hiatus hernia. A retrospective analysis has been made of 19 persons with gastric ulceration in hiatus hernias. Eleven patients presented with acute upper gastrointestinal haemorrhage and three with chronic anaemia. Seventeen had experienced previous abdominal discomfort or heartburn. Diagnosis of ulceration was achieved by endoscopy in 16 instances and by radiography in six instances. A chronic ulcer was found in 10 patients, a discrete acute ulcer in three and multiple erosions in six. Twelve of the hernias were sliding in type and irreducible, in contrast to previous reports that gastric ulceration is most frequent in paracoesophageal hernias. Conservative management resulted in complete healing of the ulcer in the majority of patients. Continued bleeding or failure of the ulcer to heal necessitated surgical intervention in six patients.", "PMID": 979849}
{"id": "pubmed23n0032_12248", "title": "Volvulus of the transverse colon in an Australian aboriginal.", "content": "A case of volvulus of the transverse colon in an elderly female Australian Aboriginal is reported. The rarity of the condition is stressed and the radiological features are discussed. Reference is also made to the geographical distribution of all types of volulus of the colon, and to the apparently high incidence of the condition in Aboriginal patients admitted to the Darwin Hospital.", "contents": "Volvulus of the transverse colon in an Australian aboriginal. A case of volvulus of the transverse colon in an elderly female Australian Aboriginal is reported. The rarity of the condition is stressed and the radiological features are discussed. Reference is also made to the geographical distribution of all types of volulus of the colon, and to the apparently high incidence of the condition in Aboriginal patients admitted to the Darwin Hospital.", "PMID": 979850}
{"id": "pubmed23n0032_12249", "title": "Fatal respiratory obstruction due to round worm.", "content": "Respiratory obstruction caused by a round worm is rate. A fatal case of the complication in a three-year-old child was encountered at the Nehru Hospital attached to the Postgraduate Institute of Medical Education and Research, Chandigarh, India.", "contents": "Fatal respiratory obstruction due to round worm. Respiratory obstruction caused by a round worm is rate. A fatal case of the complication in a three-year-old child was encountered at the Nehru Hospital attached to the Postgraduate Institute of Medical Education and Research, Chandigarh, India.", "PMID": 979851}
{"id": "pubmed23n0032_12250", "title": "Hospital staffing and hospital costs.", "content": "A comparative study of costs per bed per day in teaching hospitals affiliated with Monash University compared with large non-teaching metropolitan hospitals (1964 to 1974) shows they are much higher in teaching hospitals. There is no evidence that this is due to the additional costs arising from the clinical schools. Research in the teaching hospitals and the accompanying high professional standards and demands on services are major factors accounting for the difference. Over the decade studied, the resident staff have increased by 77% and other salaried staff by 24%. The index of expenditure for the three teaching hospitals in the decade has increased by 386%.", "contents": "Hospital staffing and hospital costs. A comparative study of costs per bed per day in teaching hospitals affiliated with Monash University compared with large non-teaching metropolitan hospitals (1964 to 1974) shows they are much higher in teaching hospitals. There is no evidence that this is due to the additional costs arising from the clinical schools. Research in the teaching hospitals and the accompanying high professional standards and demands on services are major factors accounting for the difference. Over the decade studied, the resident staff have increased by 77% and other salaried staff by 24%. The index of expenditure for the three teaching hospitals in the decade has increased by 386%.", "PMID": 979854}
{"id": "pubmed23n0032_12251", "title": "[Statistics and comparative pharmacokinetics (author's transl)].", "content": "An example stresses the point of well established routines, which are usual in comparative pharmacokinetics today. It seems to be necessary to analyse the problem carefully, before starting any pharmacokinetical trial.", "contents": "[Statistics and comparative pharmacokinetics (author's transl)]. An example stresses the point of well established routines, which are usual in comparative pharmacokinetics today. It seems to be necessary to analyse the problem carefully, before starting any pharmacokinetical trial.", "PMID": 979869}
{"id": "pubmed23n0032_12252", "title": "[Renal thrombotic microangiopathy with benign hypertension and uremia secondary to oral contraceptives (author's transl)].", "content": "A 34-year-old woman developed uremia secondary to severe renal thrombotic microangiopathy after 3 years intake of oral contraceptives. In this particular case manifestation of end stage renal failure was preceded by an unusually long lasting period of nine months with benign hypertension. Even during the final stage (3-4 weeks) prior to complete development of uremia only once hemolysis but no malignant hypertension was observed. Only close long term follow up including renal biopsy and subtile functional tests may provide information whether and/or when hypertension due to oral contraceptives turns to become--at least in part--renal hypertension and also becomes persistent. This observation does not give evidence that benign hypertension causes renovascular damage and thus renal failure. Plasma renin activity was found to be basically elevated and furthermore stimulated e.g. by dialyses. However, this single observation does not permit any conclusion about a pathogenetic role of renin in creating hypertension by e.g. renal vasoconstriction or--despite hypertension--collapse of the capillary network.", "contents": "[Renal thrombotic microangiopathy with benign hypertension and uremia secondary to oral contraceptives (author's transl)]. A 34-year-old woman developed uremia secondary to severe renal thrombotic microangiopathy after 3 years intake of oral contraceptives. In this particular case manifestation of end stage renal failure was preceded by an unusually long lasting period of nine months with benign hypertension. Even during the final stage (3-4 weeks) prior to complete development of uremia only once hemolysis but no malignant hypertension was observed. Only close long term follow up including renal biopsy and subtile functional tests may provide information whether and/or when hypertension due to oral contraceptives turns to become--at least in part--renal hypertension and also becomes persistent. This observation does not give evidence that benign hypertension causes renovascular damage and thus renal failure. Plasma renin activity was found to be basically elevated and furthermore stimulated e.g. by dialyses. However, this single observation does not permit any conclusion about a pathogenetic role of renin in creating hypertension by e.g. renal vasoconstriction or--despite hypertension--collapse of the capillary network.", "PMID": 979870}
{"id": "pubmed23n0032_12253", "title": "[Psychological and physiological effects of a 5-week ergometer training in healthy young men (author's transl)].", "content": "40 healthy male students were randomly assigned to either the exercise or control group. The latter was asked not to alter their common physical activity while the exercise group trained three times weekly on the bicycle ergometer for 15 minutes with a constant heart rate of 140bpm. At the beginning and after five weeks physiological data comprising cardiovascular and pulmonary responses at rest and under submaximal ergometric exercise were assessed. Additional data included psychological achievement tests, self reports of personality dimensions and frequency of physical complaints. Results indicated a marked increase in physical fitness for the training group improving the work load from 158 watt to 197 watt at constant heart rate. At rest and especially at submaximal work load there was an improvement of the economy of the cardiovascular and respiratory system. Contrary to these findings there were no changes or impairment in the psychological achievement tests, measuring concentration. Compared with the control group self reports of personality dimensions did not change except for a tendency to more extrovert behavior in the exercise group. Unexpectedly, the frequency of physical complaints did not decrease. The reason for this discrepancy is discussed.", "contents": "[Psychological and physiological effects of a 5-week ergometer training in healthy young men (author's transl)]. 40 healthy male students were randomly assigned to either the exercise or control group. The latter was asked not to alter their common physical activity while the exercise group trained three times weekly on the bicycle ergometer for 15 minutes with a constant heart rate of 140bpm. At the beginning and after five weeks physiological data comprising cardiovascular and pulmonary responses at rest and under submaximal ergometric exercise were assessed. Additional data included psychological achievement tests, self reports of personality dimensions and frequency of physical complaints. Results indicated a marked increase in physical fitness for the training group improving the work load from 158 watt to 197 watt at constant heart rate. At rest and especially at submaximal work load there was an improvement of the economy of the cardiovascular and respiratory system. Contrary to these findings there were no changes or impairment in the psychological achievement tests, measuring concentration. Compared with the control group self reports of personality dimensions did not change except for a tendency to more extrovert behavior in the exercise group. Unexpectedly, the frequency of physical complaints did not decrease. The reason for this discrepancy is discussed.", "PMID": 979871}
{"id": "pubmed23n0032_12254", "title": "[Coincidence of two carcinomata with excessive high level of serumestrogen (author's transl)].", "content": "A case of an unusual combination of a metastasing bronchus cancer, a non-metastasing cancer of prostata and an excessive high level of serum-estrogen is described. This casuistic gives rise to some more general discussion of the pathogenesis and differential diagnosis of paraneoplastic endocrinopathies.", "contents": "[Coincidence of two carcinomata with excessive high level of serumestrogen (author's transl)]. A case of an unusual combination of a metastasing bronchus cancer, a non-metastasing cancer of prostata and an excessive high level of serum-estrogen is described. This casuistic gives rise to some more general discussion of the pathogenesis and differential diagnosis of paraneoplastic endocrinopathies.", "PMID": 979872}
{"id": "pubmed23n0032_12255", "title": "[Malignant tumors of the colon: statistical and clinical aspects (author's transl)].", "content": "Between 1961 and 1974 at the University Hospital Surgical Clinic at Innsbruck, 991 cases of malignant tumors of the colon have been treated. Statistically a significant increase is proved in the number of these patients above the age of fifty in the Tyrolean population during this period. On the other hand the percentage of colon-tumors is decreasing with the age in patients younger than fifty. 412 out of these 991 cases could be analysed very carefully. The malignant tumors have been studied, among other respects, in relation to the localisation in the particular part of the colon, the frequency in the different ages and sexes. Specially the symptoms due to the particular localisation of the tumor are stressed. It is also noticed that the long case history, mainly because of the patients' own negligence, plays a decisive part in the unsatisfactoring number of cured. Some measures are mentioned, which with the so far got knowledge and science can improve the final therapeutic results.", "contents": "[Malignant tumors of the colon: statistical and clinical aspects (author's transl)]. Between 1961 and 1974 at the University Hospital Surgical Clinic at Innsbruck, 991 cases of malignant tumors of the colon have been treated. Statistically a significant increase is proved in the number of these patients above the age of fifty in the Tyrolean population during this period. On the other hand the percentage of colon-tumors is decreasing with the age in patients younger than fifty. 412 out of these 991 cases could be analysed very carefully. The malignant tumors have been studied, among other respects, in relation to the localisation in the particular part of the colon, the frequency in the different ages and sexes. Specially the symptoms due to the particular localisation of the tumor are stressed. It is also noticed that the long case history, mainly because of the patients' own negligence, plays a decisive part in the unsatisfactoring number of cured. Some measures are mentioned, which with the so far got knowledge and science can improve the final therapeutic results.", "PMID": 979873}
{"id": "pubmed23n0032_12256", "title": "[Acute leukemia as terminal stage of Hodgkin's disease (author's transl)].", "content": "In a 61 year-old female patient with Hodgkin's disease treated with cytotoxic drugs and radiation therapy acute undifferentiated leukemia developed 10 years later. The possibility of a carcinogenic effect of the cytotoxic and actinic therapy is discussed. Leukemia complicating the course of Hodgkin's disease must be considered an independent disease and not a iatrogenic transformation of a preexisting lesion.", "contents": "[Acute leukemia as terminal stage of Hodgkin's disease (author's transl)]. In a 61 year-old female patient with Hodgkin's disease treated with cytotoxic drugs and radiation therapy acute undifferentiated leukemia developed 10 years later. The possibility of a carcinogenic effect of the cytotoxic and actinic therapy is discussed. Leukemia complicating the course of Hodgkin's disease must be considered an independent disease and not a iatrogenic transformation of a preexisting lesion.", "PMID": 979874}
{"id": "pubmed23n0032_12257", "title": "[Etiology of shock lung: case report (author's transl)].", "content": "In a woman, 29, two times resection and anastomosis of the small intestine because of extended infarction was performed. Following peritonitis and septic shock a progressive respiratory insufficiency developed and patient died 48 hours later despite intensive ventilatory treatment. Autopsy findings were manifold vascular damage (aorta) and thromboembolisms in mesenteric vessels and iliac veins too. Patient received oral contraceptives over several years, changed the preparation previously and contraceptive therapy was continued with another one. Thromboembolic complications may understood as undesirable side effects of the contraceptives. By microscopy of lung thrombosis in capillaries was seen and in small arteries too, as embolism carried by the blood stream. In pathogenesis of shock lung macrothrombosis as an aetiologic factor is to be discussed.", "contents": "[Etiology of shock lung: case report (author's transl)]. In a woman, 29, two times resection and anastomosis of the small intestine because of extended infarction was performed. Following peritonitis and septic shock a progressive respiratory insufficiency developed and patient died 48 hours later despite intensive ventilatory treatment. Autopsy findings were manifold vascular damage (aorta) and thromboembolisms in mesenteric vessels and iliac veins too. Patient received oral contraceptives over several years, changed the preparation previously and contraceptive therapy was continued with another one. Thromboembolic complications may understood as undesirable side effects of the contraceptives. By microscopy of lung thrombosis in capillaries was seen and in small arteries too, as embolism carried by the blood stream. In pathogenesis of shock lung macrothrombosis as an aetiologic factor is to be discussed.", "PMID": 979877}
{"id": "pubmed23n0032_12258", "title": "[Sj\u00f6gren's syndrome with pulmonary fibrosis. A case report (author's transl)].", "content": "The case is pointing to two remarkable facts: 1. Sj\u00f6gren's syndrome may also be proceeding--without demonstrating any inflammatory rheumatic symptoms--with an isolated pulmonary fibrosis, as it had been evident in another case mentioned. 2. In certain circumstances, it may be considerably difficult to differentiate, with reliable certainty, the morphological changes in the salivary gland from those of a malignant lymphoma. Besides, also in literature there are reports concerning malignant degeneration turning into malignant lymphomata [1,8] in cases of Sj\u00f6gren's syndrome.", "contents": "[Sj\u00f6gren's syndrome with pulmonary fibrosis. A case report (author's transl)]. The case is pointing to two remarkable facts: 1. Sj\u00f6gren's syndrome may also be proceeding--without demonstrating any inflammatory rheumatic symptoms--with an isolated pulmonary fibrosis, as it had been evident in another case mentioned. 2. In certain circumstances, it may be considerably difficult to differentiate, with reliable certainty, the morphological changes in the salivary gland from those of a malignant lymphoma. Besides, also in literature there are reports concerning malignant degeneration turning into malignant lymphomata [1,8] in cases of Sj\u00f6gren's syndrome.", "PMID": 979878}
{"id": "pubmed23n0032_12259", "title": "[Quantitative tracer examination about the deposition of inhaled aerosols (author's transl)].", "content": "The topical distribution of inhaled aerosols produced by customary jet nebulizers has been measured scintigraphically. Corrections for absorption and stray effects with experimentally determined factors allowed to evaluate the quantities of deposited aerosol for the head, chest and abdominal regions. Highest aerosol intakes were found with slow inhalation through the mouth. The deposition decreased at higher respiration rates with a further diminution when inhalation takes place through the nose. The individuals had characteristic patterns of aerosol distribution resulting in more pronounced deposition differences than those caused by the mode of inhalation.", "contents": "[Quantitative tracer examination about the deposition of inhaled aerosols (author's transl)]. The topical distribution of inhaled aerosols produced by customary jet nebulizers has been measured scintigraphically. Corrections for absorption and stray effects with experimentally determined factors allowed to evaluate the quantities of deposited aerosol for the head, chest and abdominal regions. Highest aerosol intakes were found with slow inhalation through the mouth. The deposition decreased at higher respiration rates with a further diminution when inhalation takes place through the nose. The individuals had characteristic patterns of aerosol distribution resulting in more pronounced deposition differences than those caused by the mode of inhalation.", "PMID": 979879}
{"id": "pubmed23n0032_12260", "title": "Relevance of dimethylamine to mechanism studies of DIC (DTIC, NSC 45388).", "content": "Monolayer cultures of Chinese hamster ovary (CHO) cells take up the photo-decomposition products of DIC more readily than DIC itself. Dimethylamine, an immediate product of this degradative pathway, can ultimately become associated with the DNA, RNA, and protein of the cells as demonstrated by selective enzymatic degradation of macromolecules and isopycnic centrifugation. The relevance of these observations to mechanism studies of DIC is discussed.", "contents": "Relevance of dimethylamine to mechanism studies of DIC (DTIC, NSC 45388). Monolayer cultures of Chinese hamster ovary (CHO) cells take up the photo-decomposition products of DIC more readily than DIC itself. Dimethylamine, an immediate product of this degradative pathway, can ultimately become associated with the DNA, RNA, and protein of the cells as demonstrated by selective enzymatic degradation of macromolecules and isopycnic centrifugation. The relevance of these observations to mechanism studies of DIC is discussed.", "PMID": 979914}
{"id": "pubmed23n0032_12261", "title": "A study of intermittent alternating drug program reinduction therapy on the frequency and duration of response in adult acute leukemia.", "content": "Of 41 adults with a diagnosis of acute leukemia that were randomized for induction therapy in combination with methotrexate, 6-MP, vincristine and prednisone (POMP) versus a combination of cytosine arabinoside, cytoxan, vincristine and prednisone (COAP), 23 (56%) patients achieved a complete remission. During remission, patients received consolidation therapy with the three courses of remission induction regimen that they had not received initially. They then received daunomycin (three courses) and L-asparaginase and were then maintained for two years with their induction therapy. The median duration of survival for all patients was 40 weeks; the median duration of survival of those patients that responded to chemotherapy was 80 weeks. There was no significant difference between the two induction regimens with regard to complete remission more than four and one half years from diagnosis and two and one half years from discontinuation of all therapy.", "contents": "A study of intermittent alternating drug program reinduction therapy on the frequency and duration of response in adult acute leukemia. Of 41 adults with a diagnosis of acute leukemia that were randomized for induction therapy in combination with methotrexate, 6-MP, vincristine and prednisone (POMP) versus a combination of cytosine arabinoside, cytoxan, vincristine and prednisone (COAP), 23 (56%) patients achieved a complete remission. During remission, patients received consolidation therapy with the three courses of remission induction regimen that they had not received initially. They then received daunomycin (three courses) and L-asparaginase and were then maintained for two years with their induction therapy. The median duration of survival for all patients was 40 weeks; the median duration of survival of those patients that responded to chemotherapy was 80 weeks. There was no significant difference between the two induction regimens with regard to complete remission more than four and one half years from diagnosis and two and one half years from discontinuation of all therapy.", "PMID": 979915}
{"id": "pubmed23n0032_12262", "title": "Psychological evaluation and management of pediatric oncology patients in protected environments.", "content": "Specific aspects of psychological investigation and management of pediatric patients with widely disseminated solid tumors treated in semiportable, laminar airflow patient isolators are discussed. Information was obtained prospectively from a psychosocial study c-ordinated with a medical investigation of the use of protected environments (PE) employing barrier isolation as an adjunct to intensive anticancer therapy. Included are description of the development of psychological criteria for patient eligibility, psychometric evaluation, longitudinal behavioral observation, pre-entry and predischarge orientation techniques, as well as approaches to the ongoing management of patients and families. No debilitating psychological disturbance has been observed in these patients, and no patients have had to be removed for psychological reasons. Specific transitory psychological changes are noted as are the problems encountered by staff members functioning in such a setting. The adoption of a well-coordinated psychosocial program of investigation and clinical intervention has proved useful in maximizing patient adjustment to prolonged treatment in protected environments.", "contents": "Psychological evaluation and management of pediatric oncology patients in protected environments. Specific aspects of psychological investigation and management of pediatric patients with widely disseminated solid tumors treated in semiportable, laminar airflow patient isolators are discussed. Information was obtained prospectively from a psychosocial study c-ordinated with a medical investigation of the use of protected environments (PE) employing barrier isolation as an adjunct to intensive anticancer therapy. Included are description of the development of psychological criteria for patient eligibility, psychometric evaluation, longitudinal behavioral observation, pre-entry and predischarge orientation techniques, as well as approaches to the ongoing management of patients and families. No debilitating psychological disturbance has been observed in these patients, and no patients have had to be removed for psychological reasons. Specific transitory psychological changes are noted as are the problems encountered by staff members functioning in such a setting. The adoption of a well-coordinated psychosocial program of investigation and clinical intervention has proved useful in maximizing patient adjustment to prolonged treatment in protected environments.", "PMID": 979916}
{"id": "pubmed23n0032_12263", "title": "Liquid xenon scintillators for imaging of positron emitters.", "content": "The current understanding of xenon scintillation physics is summarized and keyed to the use of xenon as a gamma-ray detector in medical radioisotope imaging systems. Liquid xenon has a short scintillation pulse (approximately 10(8) sec) and high gamma-ray absorption and scintillation efficiencies. The fast pulse may facilitate imaging in vivo distributions of hot positron sources and allow recovery of additional spatial information by time-of-flight techniques. We begin by describing our own study of the feasibility of making a practical positron scanning system, and consider the problems of scintillation decay time, linearity, efficiency, purity, and electricfield amplifcation. The prospects for a practical instrument are considered.", "contents": "Liquid xenon scintillators for imaging of positron emitters. The current understanding of xenon scintillation physics is summarized and keyed to the use of xenon as a gamma-ray detector in medical radioisotope imaging systems. Liquid xenon has a short scintillation pulse (approximately 10(8) sec) and high gamma-ray absorption and scintillation efficiencies. The fast pulse may facilitate imaging in vivo distributions of hot positron sources and allow recovery of additional spatial information by time-of-flight techniques. We begin by describing our own study of the feasibility of making a practical positron scanning system, and consider the problems of scintillation decay time, linearity, efficiency, purity, and electricfield amplifcation. The prospects for a practical instrument are considered.", "PMID": 979917}
{"id": "pubmed23n0032_12264", "title": "Comparisons of calorimetric and ionometric measurements in graphite irradiated with electrons from 15 to 50 MeV.", "content": "Extensive experimental comparisons of calorimetric and ionometric measurements have been made that cover a broader range of electron energies and depths in graphite than previously reported. Electron beams of 15, 20, 25, 30, 40, and 50 MeV were used. Calorimetric absorbed-dose measurements and ionometric specific-charge measurements in air were compared in graphite at depths from 1 to 51 g/cm2. The medium was irradiated with uncollimated electron beams produced by scattering after passing through a 0.1-g/cm2 aluminum vacuum window, various thicknesses of lead foils, and air. The variation in the quotient of the two measurements was studied as a function of lead-foil thickness, depth in the medium, beam energy, foil-to-detector distance, and off-axis distance. These studies permitted the measurements to be corrected and compared with theoretical calculations that assume a broad medium irradiated with broad, parallel, monoenergetic electron beams. The overall experimental uncertainty is estimated to be 1%. The results are generally in good agreement with theoretical and experimental results of other investigators. The calorimeter received close to 1 Mrad during preliminary measurements and from 1 to 2 Mrad during the measurements reported. The results showed no detectable heat defect in graphite after prolonged periods of exposing the calorimeter to air at atmospheric pressure.", "contents": "Comparisons of calorimetric and ionometric measurements in graphite irradiated with electrons from 15 to 50 MeV. Extensive experimental comparisons of calorimetric and ionometric measurements have been made that cover a broader range of electron energies and depths in graphite than previously reported. Electron beams of 15, 20, 25, 30, 40, and 50 MeV were used. Calorimetric absorbed-dose measurements and ionometric specific-charge measurements in air were compared in graphite at depths from 1 to 51 g/cm2. The medium was irradiated with uncollimated electron beams produced by scattering after passing through a 0.1-g/cm2 aluminum vacuum window, various thicknesses of lead foils, and air. The variation in the quotient of the two measurements was studied as a function of lead-foil thickness, depth in the medium, beam energy, foil-to-detector distance, and off-axis distance. These studies permitted the measurements to be corrected and compared with theoretical calculations that assume a broad medium irradiated with broad, parallel, monoenergetic electron beams. The overall experimental uncertainty is estimated to be 1%. The results are generally in good agreement with theoretical and experimental results of other investigators. The calorimeter received close to 1 Mrad during preliminary measurements and from 1 to 2 Mrad during the measurements reported. The results showed no detectable heat defect in graphite after prolonged periods of exposing the calorimeter to air at atmospheric pressure.", "PMID": 979918}
{"id": "pubmed23n0032_12265", "title": "State-of-the-art of single-transducer ultrasonic imaging technology.", "content": "This paper attempts to take note of some recent improvements in ultrasound  echo-ranging technology and make some observations on the single versus multiple-transducer element approach. It would appear at this time that the problems encountered with visualization systems may not be solved by using multiple transducers per se, and that the unique advantages that accrue to the multiple-transducer systems have yet to demonstrate that they fulfill and important clinical need.", "contents": "State-of-the-art of single-transducer ultrasonic imaging technology. This paper attempts to take note of some recent improvements in ultrasound  echo-ranging technology and make some observations on the single versus multiple-transducer element approach. It would appear at this time that the problems encountered with visualization systems may not be solved by using multiple transducers per se, and that the unique advantages that accrue to the multiple-transducer systems have yet to demonstrate that they fulfill and important clinical need.", "PMID": 979919}
{"id": "pubmed23n0032_12266", "title": "State-of-the-art in two-dimensional ultrasonic transducer array technology.", "content": "Attention is given to means of sensing ultrasonic energy distributions over an area. Under the restraints appropiate to real-time imaging of deep body organs, piezoelectric arrays offer the most promising method. Adaptation of integrated circuit techniques to array assembly permits very large arrays of small elements to be batch fabricated. Further, special semiconductor switching devices specifically designed for addressing the array have been produced and applied. These permit both the passage of weak received signals and the application of voltage and current levels sufficient for transmission without significant disturbance of the basic piezoelectric element properties. Emphasis is placed on the complete data acquisition, processing, and display flexibility that arises from an array capability. Operation becomes possible in any of the presently used A or time motion (TM), in real time B or C scan, or in novel scan patterns adapted to specific organs. Further development with acoustic and electronic focusing, taking advantage of the unique possibilities obtained with the bidirectional array structure, is discussed.", "contents": "State-of-the-art in two-dimensional ultrasonic transducer array technology. Attention is given to means of sensing ultrasonic energy distributions over an area. Under the restraints appropiate to real-time imaging of deep body organs, piezoelectric arrays offer the most promising method. Adaptation of integrated circuit techniques to array assembly permits very large arrays of small elements to be batch fabricated. Further, special semiconductor switching devices specifically designed for addressing the array have been produced and applied. These permit both the passage of weak received signals and the application of voltage and current levels sufficient for transmission without significant disturbance of the basic piezoelectric element properties. Emphasis is placed on the complete data acquisition, processing, and display flexibility that arises from an array capability. Operation becomes possible in any of the presently used A or time motion (TM), in real time B or C scan, or in novel scan patterns adapted to specific organs. Further development with acoustic and electronic focusing, taking advantage of the unique possibilities obtained with the bidirectional array structure, is discussed.", "PMID": 979920}
{"id": "pubmed23n0032_12267", "title": "Analysis of in vivo volume measurements obtained with diagnostic ultrasound.", "content": "A study was carried out to investigate the errors involved in obtaining volumes by means of ultrasound echograms and a small computer. The work was done  on live pigs' kidneys (130-200 cm3). The errors include those associated with the scanner itself, those invloved in the interpretation of the contour boundaries, and those relating to the algorithms used to determine the volumes from the contours. An average deviation of 25.4 cm3 from actual volumes was found, while 58% of the measurements were within +/-10%. Computer programs allowing for the display of contours and intercepts of corresponding orthogonal scan planes were used to reanalyze the data resulting in an average deviation of 14.7 cm3 with 78% of  the data resulting inan average deviation of 14.7 cm3 with 78% of the measurements with \"/-10%.", "contents": "Analysis of in vivo volume measurements obtained with diagnostic ultrasound. A study was carried out to investigate the errors involved in obtaining volumes by means of ultrasound echograms and a small computer. The work was done  on live pigs' kidneys (130-200 cm3). The errors include those associated with the scanner itself, those invloved in the interpretation of the contour boundaries, and those relating to the algorithms used to determine the volumes from the contours. An average deviation of 25.4 cm3 from actual volumes was found, while 58% of the measurements were within +/-10%. Computer programs allowing for the display of contours and intercepts of corresponding orthogonal scan planes were used to reanalyze the data resulting in an average deviation of 14.7 cm3 with 78% of  the data resulting inan average deviation of 14.7 cm3 with 78% of the measurements with \"/-10%.", "PMID": 979921}
{"id": "pubmed23n0032_12268", "title": "Spectroscopy of diagnostic x rays by a Compton-scatter method.", "content": "A method is described for measuring the energy spectra of diagnostic x rays using a high-resolution intrinsic germanium spectrometer. The method is applicable over the wide range of x-ray exposure rates normally used in radiography. Reduction of the high x-ray beam intensity to a level acceptable to the germanium spectrometer is achieved by measuring the spectrum of photons scattered through 90 degrees by a lucite disc. From this measured spectrum the primary spectrum is obtained by calculation. Some typical x-ray spectra are presented.", "contents": "Spectroscopy of diagnostic x rays by a Compton-scatter method. A method is described for measuring the energy spectra of diagnostic x rays using a high-resolution intrinsic germanium spectrometer. The method is applicable over the wide range of x-ray exposure rates normally used in radiography. Reduction of the high x-ray beam intensity to a level acceptable to the germanium spectrometer is achieved by measuring the spectrum of photons scattered through 90 degrees by a lucite disc. From this measured spectrum the primary spectrum is obtained by calculation. Some typical x-ray spectra are presented.", "PMID": 979923}
{"id": "pubmed23n0032_12269", "title": "Modified transit dosementer system for 60Co rotational therapy.", "content": "The transit dosemeter has been used in obtaining inhomogeneity corrections for 60Co rotational therapy. A modified approach is presented which features a rapid electronic system for determining the average tissue-air ratio (TAR) for a 360 degree rotation. Experimental results and early clinical experience show that this system is capable of providing a TAR value directly, and more rapidly. It can be readily incorporated in a therapy system which already includes a transit dosemeter.", "contents": "Modified transit dosementer system for 60Co rotational therapy. The transit dosemeter has been used in obtaining inhomogeneity corrections for 60Co rotational therapy. A modified approach is presented which features a rapid electronic system for determining the average tissue-air ratio (TAR) for a 360 degree rotation. Experimental results and early clinical experience show that this system is capable of providing a TAR value directly, and more rapidly. It can be readily incorporated in a therapy system which already includes a transit dosemeter.", "PMID": 979924}
{"id": "pubmed23n0032_12270", "title": "Digital display plug-in module for the Victoreen model 555 integral and rate exposuremeter.", "content": "A digital display plug-in for use with the Victoreen 555 exposuremeter is described. The device is a readout subsystem for displaying numerical and dimensional information in a clear, legible form, with both probe and range scale factors accounted for in the display itself. In addition to facilitating data gathering with the 555, the device circumvents nonlinearity in the analog meter movement. Minor modifications required of the 555 mainframe are described.", "contents": "Digital display plug-in module for the Victoreen model 555 integral and rate exposuremeter. A digital display plug-in for use with the Victoreen 555 exposuremeter is described. The device is a readout subsystem for displaying numerical and dimensional information in a clear, legible form, with both probe and range scale factors accounted for in the display itself. In addition to facilitating data gathering with the 555, the device circumvents nonlinearity in the analog meter movement. Minor modifications required of the 555 mainframe are described.", "PMID": 979926}
{"id": "pubmed23n0032_12271", "title": "Calibration methods for measuring splenic sequestration by external scanning.", "content": "Conventional methods for measuring splenic sequestration of labeled cells rely on stationary probe counting over the liver and spleen. A quantitative spleen-scanning method is proposed as a means of performing a more accurate diagnosis of the degree of splenic sequestration. Improved methods for calibrating a rectilinear scanner employing \"constant resolutions\" collimators, designed for the in vivo measurement, are reported, Clinical evaluation of this method was performed by scanning several patients scheduled for splenectomy. Good correlation between in vivo and in vitro measurements of splenic radioactivity for 29 patients was obtained. A least-squares fit to the data yielded a coefficient of determination r2=0.93.", "contents": "Calibration methods for measuring splenic sequestration by external scanning. Conventional methods for measuring splenic sequestration of labeled cells rely on stationary probe counting over the liver and spleen. A quantitative spleen-scanning method is proposed as a means of performing a more accurate diagnosis of the degree of splenic sequestration. Improved methods for calibrating a rectilinear scanner employing \"constant resolutions\" collimators, designed for the in vivo measurement, are reported, Clinical evaluation of this method was performed by scanning several patients scheduled for splenectomy. Good correlation between in vivo and in vitro measurements of splenic radioactivity for 29 patients was obtained. A least-squares fit to the data yielded a coefficient of determination r2=0.93.", "PMID": 979922}
{"id": "pubmed23n0032_12272", "title": "Calibration instrumentation used by the AAPM Radiological Physics Center.", "content": "The Keithley model 602 electrometer-Farmer ionization chamber combination has served as the standard dosimetry system for calibration measurements made by the AAPM Radiological Physics Center at 156 different institutions. The constancy of this system is compared to that for the Baldwin-Farmer Secondary Standard Dosemeter type 2502, and is based on measurements made over a 3-yr period on a Cobalt-60 gamma-ray source. A Strontium-90 check source is also shown to be a reliable standard, and frequent checks with such a source can prevent serious calibration errors.", "contents": "Calibration instrumentation used by the AAPM Radiological Physics Center. The Keithley model 602 electrometer-Farmer ionization chamber combination has served as the standard dosimetry system for calibration measurements made by the AAPM Radiological Physics Center at 156 different institutions. The constancy of this system is compared to that for the Baldwin-Farmer Secondary Standard Dosemeter type 2502, and is based on measurements made over a 3-yr period on a Cobalt-60 gamma-ray source. A Strontium-90 check source is also shown to be a reliable standard, and frequent checks with such a source can prevent serious calibration errors.", "PMID": 979927}
{"id": "pubmed23n0032_12273", "title": "Heuristic model for understanding x-ray film characteristics.", "content": "A simple, heuristic model of a photographic emulsion is described for the purpose of illustrating the fundamental physical processes and emulsion properties which determine the characteristics of an x-ray film (viz., the shape of the H - D curve, film gamma, and film speed). By means of this model, it is shown that the contrast multiplication afforded by an x-ray film (i.e., a film gamma greater than unity) is a direct result of the exponential attenuation of the viewing light by the developed film, and that film gamma is proportional to grain size, grain density, and emulsion thickness. The difference in the H - D curve that is observed when the same film is exposed to light from an intensifying screen or directly to x rays is also predicted by the model.", "contents": "Heuristic model for understanding x-ray film characteristics. A simple, heuristic model of a photographic emulsion is described for the purpose of illustrating the fundamental physical processes and emulsion properties which determine the characteristics of an x-ray film (viz., the shape of the H - D curve, film gamma, and film speed). By means of this model, it is shown that the contrast multiplication afforded by an x-ray film (i.e., a film gamma greater than unity) is a direct result of the exponential attenuation of the viewing light by the developed film, and that film gamma is proportional to grain size, grain density, and emulsion thickness. The difference in the H - D curve that is observed when the same film is exposed to light from an intensifying screen or directly to x rays is also predicted by the model.", "PMID": 979925}
{"id": "pubmed23n0032_12274", "title": "Evaluation of nuclear-reactor-produced iodine-123.", "content": "Iodine-123 has such great potential to nuclear medicine that all possible production methods should be considered. In this report an experimental study related to 123l production at a high-intensity fast flux reactor using the reaction 124Xe(n,2n)123Xe is considered. The conclusion is that 123l could be made in small quantities and the cost would be higher than the cyclotron methods presently used.", "contents": "Evaluation of nuclear-reactor-produced iodine-123. Iodine-123 has such great potential to nuclear medicine that all possible production methods should be considered. In this report an experimental study related to 123l production at a high-intensity fast flux reactor using the reaction 124Xe(n,2n)123Xe is considered. The conclusion is that 123l could be made in small quantities and the cost would be higher than the cyclotron methods presently used.", "PMID": 979929}
{"id": "pubmed23n0032_12275", "title": "Polynomial and power law of central axis backscatter factors for external and gamma-ray sources.", "content": "A third-degree polynomial and power-law analysis with the method of least-squares fit are computed for backscatter factor with a continuous variation of field size and different beam quality of radiation from 1.0-mm Al external x ray to 60Co gamma ray. Three coefficients are required for a third-degree polynomial and two coefficients for power law method of each beam quality.", "contents": "Polynomial and power law of central axis backscatter factors for external and gamma-ray sources. A third-degree polynomial and power-law analysis with the method of least-squares fit are computed for backscatter factor with a continuous variation of field size and different beam quality of radiation from 1.0-mm Al external x ray to 60Co gamma ray. Three coefficients are required for a third-degree polynomial and two coefficients for power law method of each beam quality.", "PMID": 979928}
{"id": "pubmed23n0032_12276", "title": "Organisms associated with abortion and reproductive problems in cattle.", "content": "In 14 herds totaling over 2000 cattle the bacterial species most frequently isolated from the vagina, fetus, and fetal membranes following abortion or reproductive failure were Streptococcus, Corynebacterium, Bacillus, and Staphylococcus, less often E coli. About 5% of the total 1810 isolations included various other agents. Although most of the aborted fetuses had undergone autolysis, pulmonary, hepatic, renal, and placental lesions were observed in a few cases.", "contents": "Organisms associated with abortion and reproductive problems in cattle. In 14 herds totaling over 2000 cattle the bacterial species most frequently isolated from the vagina, fetus, and fetal membranes following abortion or reproductive failure were Streptococcus, Corynebacterium, Bacillus, and Staphylococcus, less often E coli. About 5% of the total 1810 isolations included various other agents. Although most of the aborted fetuses had undergone autolysis, pulmonary, hepatic, renal, and placental lesions were observed in a few cases.", "PMID": 979950}
{"id": "pubmed23n0032_12277", "title": "Rearrangements at the mating type locus in fission yeast.", "content": "Crosses involving the partially defective mating type mutant B102 (functional in conjugation, defective in meiosis) have confirmed the notion that, in Schizosaccharomyces pombe, certain mating type mutations can arise by transposition. A copy of the mat2P segment (specifying + mating type) is transposed and inserted into the mat1M segment (usually specifying - mating type). The mat1M segment affected by the insertion loses its former - function entirely. The - function is, however, fully regained upon excision of the transposed and inserted mat2P segment. At either position, the mat2P segments can undergo inactivations to different states of residual activity. These events can occur about as frequent as other mutations of the mating type locus (ca. 10(-4) per cell division). In certain diploid strains, such inactivations were significantly correlated with recombination. Spontaneous reversions to full activity were also observed.", "contents": "Rearrangements at the mating type locus in fission yeast. Crosses involving the partially defective mating type mutant B102 (functional in conjugation, defective in meiosis) have confirmed the notion that, in Schizosaccharomyces pombe, certain mating type mutations can arise by transposition. A copy of the mat2P segment (specifying + mating type) is transposed and inserted into the mat1M segment (usually specifying - mating type). The mat1M segment affected by the insertion loses its former - function entirely. The - function is, however, fully regained upon excision of the transposed and inserted mat2P segment. At either position, the mat2P segments can undergo inactivations to different states of residual activity. These events can occur about as frequent as other mutations of the mating type locus (ca. 10(-4) per cell division). In certain diploid strains, such inactivations were significantly correlated with recombination. Spontaneous reversions to full activity were also observed.", "PMID": 979958}
{"id": "pubmed23n0032_12278", "title": "Thermal inactivation of maltase and its application to temperature-sensitive mutants of yeast.", "content": "Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, lack the maltase of intermediate stability. This deficiency appears to be the basis of the temperature sensitivity, and it is concluded that these strains carry a mutation in the structural gene for that maltase.", "contents": "Thermal inactivation of maltase and its application to temperature-sensitive mutants of yeast. Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, lack the maltase of intermediate stability. This deficiency appears to be the basis of the temperature sensitivity, and it is concluded that these strains carry a mutation in the structural gene for that maltase.", "PMID": 979959}
{"id": "pubmed23n0032_12279", "title": "The dependence of HNO2 mutagenesis in phage T4 on ligase and the lack of dependence of 2AP mutagenesis on repair functions.", "content": "A temperature sensitive ligase allele of phage T4 reduced or eliminated HNO2 induced reversion of am mutants. Since at the temperatures used, the ligase mutant is defective in the repair of some types of lethal lesions (i.e., UV, MMS and EMS induced lesions) these results indicate that HNO2 mutagenesis may occur through a ligase dependent repair pathway. In contrast, 2AP induced mutation was not inhibited by mutants defective in the gene 30 ligase or in genes 32, 39, 41, 42, 44, 45, 46, 47, 49, 52, 56, 58-61 and v. This indicates that 2AP mutagenesis probably does not depend on a repair pathway in phage T4.", "contents": "The dependence of HNO2 mutagenesis in phage T4 on ligase and the lack of dependence of 2AP mutagenesis on repair functions. A temperature sensitive ligase allele of phage T4 reduced or eliminated HNO2 induced reversion of am mutants. Since at the temperatures used, the ligase mutant is defective in the repair of some types of lethal lesions (i.e., UV, MMS and EMS induced lesions) these results indicate that HNO2 mutagenesis may occur through a ligase dependent repair pathway. In contrast, 2AP induced mutation was not inhibited by mutants defective in the gene 30 ligase or in genes 32, 39, 41, 42, 44, 45, 46, 47, 49, 52, 56, 58-61 and v. This indicates that 2AP mutagenesis probably does not depend on a repair pathway in phage T4.", "PMID": 979960}
{"id": "pubmed23n0032_12280", "title": "Studies on the mechanism of metabolic stimulation in polymorphonuclear leukocytes during phagocytosis. Activators and inhibitors of the granule bound NADPH oxidase.", "content": "The effects of several known inhibitors and activators of peroxidase-catalyzed reactions have been studied on the NADPH oxidase activity of granules isolated from polymorphonuclear leukocytes at rest or during phagocytosis. Redogenic substances, such as ascorbate or hydroquinone, and superoxide dismutase, which are known to inhibit peroxidase-catalyzed reactions, also inhibited the NADPH oxidase activity of granules. Oxidogenic substances, such as guaiacol or resorcinol, and manganese, which are known to stimulate peroxidase-catalyzed reactions, also activated the NADPH oxidase activity of granules. Cyanide, an inhibitor of peroxidase-catalyzed reactions, inhibited the NADPH oxidase activity of granules isolated from resting leukocytes but only slightly affected that of granules isolated from phagocytosing cells, as previously reported. A list of the properties of the NADPH oxidase activity of granules and of peroxidase oxidase activity is given. The arguments in favor of and those against a possible identity of the two activities are discussed.", "contents": "Studies on the mechanism of metabolic stimulation in polymorphonuclear leukocytes during phagocytosis. Activators and inhibitors of the granule bound NADPH oxidase. The effects of several known inhibitors and activators of peroxidase-catalyzed reactions have been studied on the NADPH oxidase activity of granules isolated from polymorphonuclear leukocytes at rest or during phagocytosis. Redogenic substances, such as ascorbate or hydroquinone, and superoxide dismutase, which are known to inhibit peroxidase-catalyzed reactions, also inhibited the NADPH oxidase activity of granules. Oxidogenic substances, such as guaiacol or resorcinol, and manganese, which are known to stimulate peroxidase-catalyzed reactions, also activated the NADPH oxidase activity of granules. Cyanide, an inhibitor of peroxidase-catalyzed reactions, inhibited the NADPH oxidase activity of granules isolated from resting leukocytes but only slightly affected that of granules isolated from phagocytosing cells, as previously reported. A list of the properties of the NADPH oxidase activity of granules and of peroxidase oxidase activity is given. The arguments in favor of and those against a possible identity of the two activities are discussed.", "PMID": 979961}
{"id": "pubmed23n0032_12281", "title": "[Improved prognosis of Ewing-sarcoma by combined irradiation and chemotherapy (author's transl)].", "content": "Until recently Ewing's sarcoma has been considered the most lethal malignant bone tumor with the poorest prognosis. An 8-year-old girl with Ewing's sarcoma is described who survived without recurrent disease for nearly 5 years now. 5-year-survival rates among large groups in the literature are reported, and different kinds of therapy compared. There are indications for a tendency that combined radio- and chemotherapy probably have the best results, since in most cases at the time of diagnosis the tumor already has metastasized or, like Lichtenstein believes, Ewing's sarcoma, primarily is of multicentric origin.", "contents": "[Improved prognosis of Ewing-sarcoma by combined irradiation and chemotherapy (author's transl)]. Until recently Ewing's sarcoma has been considered the most lethal malignant bone tumor with the poorest prognosis. An 8-year-old girl with Ewing's sarcoma is described who survived without recurrent disease for nearly 5 years now. 5-year-survival rates among large groups in the literature are reported, and different kinds of therapy compared. There are indications for a tendency that combined radio- and chemotherapy probably have the best results, since in most cases at the time of diagnosis the tumor already has metastasized or, like Lichtenstein believes, Ewing's sarcoma, primarily is of multicentric origin.", "PMID": 979963}
{"id": "pubmed23n0032_12282", "title": "[Generalized progressive vaccinia in a child with primary humoral and cellular immunodeficiency (author's transl)].", "content": "A case of generalized progressive vaccinia with lethal outcome after smallpox vaccination observed in an 8 months old girl during 1968 is reported. This complication was the first sign of an underlying immune deficiency in this child. The most conspicious findings suggesting a humoral immune defect were an absent serum IgM in combination with decreased IgA and IgG levels. An additional cellular defect was suggested by a generalized hypoplasia of the thymus and the entire lymphatic system as shown during autopsy. Vaccinia virus could be found not only in skin eruptions intra vitam but also in lung, liver and brain tissue in post mortem studies.", "contents": "[Generalized progressive vaccinia in a child with primary humoral and cellular immunodeficiency (author's transl)]. A case of generalized progressive vaccinia with lethal outcome after smallpox vaccination observed in an 8 months old girl during 1968 is reported. This complication was the first sign of an underlying immune deficiency in this child. The most conspicious findings suggesting a humoral immune defect were an absent serum IgM in combination with decreased IgA and IgG levels. An additional cellular defect was suggested by a generalized hypoplasia of the thymus and the entire lymphatic system as shown during autopsy. Vaccinia virus could be found not only in skin eruptions intra vitam but also in lung, liver and brain tissue in post mortem studies.", "PMID": 979964}
{"id": "pubmed23n0032_12283", "title": "[Peritoneal dialysis for treatment of hemolytic-uremic syndrome in children (author's transl)].", "content": "After a short introduction on etiology, symptomatology and therapy the authors present 4 cases of hemolytic-uremic syndrome. All children were treated with peritoneal dialysis which was repeated many times, when the anuric-condition lasted for a long time. Indications and the technique of the peritoneal dialysis are given. The authors prove that the peritoneal dialysis therapy of hemolytic-uremic syndrome has good results at different ages.", "contents": "[Peritoneal dialysis for treatment of hemolytic-uremic syndrome in children (author's transl)]. After a short introduction on etiology, symptomatology and therapy the authors present 4 cases of hemolytic-uremic syndrome. All children were treated with peritoneal dialysis which was repeated many times, when the anuric-condition lasted for a long time. Indications and the technique of the peritoneal dialysis are given. The authors prove that the peritoneal dialysis therapy of hemolytic-uremic syndrome has good results at different ages.", "PMID": 979965}
{"id": "pubmed23n0032_12284", "title": "[Hypocomplementemic membranoproliferative glomerulonephritis in a child with ulcerative colitis (author's transl)].", "content": "A 5 1/2 year old girl with hypocomplementemic membranoproliferative glomerulonephritis suffered from severe nephrotic syndrome. Despite intensive treatment with corticosteroids and immunosuppressive drugs the clinical state deteriorated. Three years after clinical onset of the disease the girl entered our regular hemodialysis program because of terminal renal insufficiency. After two weeks of intermittent hemodialysis she presented intestinal bleeding, which could not be stopped. One week later complete ileus developed and the child died. Before the onset of melaena no occult blood or mucus could be detected in the faeces. The autopsy revealed a severe ulcerative colitis with pseudopolyposis of the whole colon. In serum specimens still available colonic antigen could be detected by means of immunodiffusion using a rabbit antiserum against fetal colonic extract. Immunofluorescence studies showed granular deposits of immunoglobulins and complement along the glomerular capillary walls suggesting an immunogenesis of the glomerulonephritis by circulating immune complexes. The possibility of an interrelationship in the pathogenesis of both diseases is discussed. It should not be excluded that immune complexes formed in excess of colonic antigen have caused or perpetuated chronic glomerulonephritis.", "contents": "[Hypocomplementemic membranoproliferative glomerulonephritis in a child with ulcerative colitis (author's transl)]. A 5 1/2 year old girl with hypocomplementemic membranoproliferative glomerulonephritis suffered from severe nephrotic syndrome. Despite intensive treatment with corticosteroids and immunosuppressive drugs the clinical state deteriorated. Three years after clinical onset of the disease the girl entered our regular hemodialysis program because of terminal renal insufficiency. After two weeks of intermittent hemodialysis she presented intestinal bleeding, which could not be stopped. One week later complete ileus developed and the child died. Before the onset of melaena no occult blood or mucus could be detected in the faeces. The autopsy revealed a severe ulcerative colitis with pseudopolyposis of the whole colon. In serum specimens still available colonic antigen could be detected by means of immunodiffusion using a rabbit antiserum against fetal colonic extract. Immunofluorescence studies showed granular deposits of immunoglobulins and complement along the glomerular capillary walls suggesting an immunogenesis of the glomerulonephritis by circulating immune complexes. The possibility of an interrelationship in the pathogenesis of both diseases is discussed. It should not be excluded that immune complexes formed in excess of colonic antigen have caused or perpetuated chronic glomerulonephritis.", "PMID": 979966}
{"id": "pubmed23n0032_12285", "title": "[Sphingomyelin lipoidosis (author's transl)].", "content": "The case of a girl is described who was 3 1/4 years of age and was admitted of the hospital because of hepato-splenomegaly. In addition to this, there were peculiar alterations in the lungs, an increase of lipoids in the blood, a characteristic alteration of the retina, and bone marrow and liver cells storing lipoid. The diagnosis of sphingomyelin lipoidosis (Niemann-Pick) was confirmed by electronmicroscopic and biochemical investigation of a percutaneous liver biopsy specimen.", "contents": "[Sphingomyelin lipoidosis (author's transl)]. The case of a girl is described who was 3 1/4 years of age and was admitted of the hospital because of hepato-splenomegaly. In addition to this, there were peculiar alterations in the lungs, an increase of lipoids in the blood, a characteristic alteration of the retina, and bone marrow and liver cells storing lipoid. The diagnosis of sphingomyelin lipoidosis (Niemann-Pick) was confirmed by electronmicroscopic and biochemical investigation of a percutaneous liver biopsy specimen.", "PMID": 979967}
{"id": "pubmed23n0032_12286", "title": "[\"Lublin syndrome\". A case of generalized haemangiomatosis of the skin, and hydrocephalus (author's transl)].", "content": "The authors describe a new syndrome published first by Polish investigators. The syndrome is called \"Lublin-syndrome\" after the original authors. Generalised haemangiomatosis of the skin and severe irreversibile cerebral damage are the essential symptoms of the disease. The assumed pathogenesis, morphological changes of the disease, and the therapeutical attempts are discussed.", "contents": "[\"Lublin syndrome\". A case of generalized haemangiomatosis of the skin, and hydrocephalus (author's transl)]. The authors describe a new syndrome published first by Polish investigators. The syndrome is called \"Lublin-syndrome\" after the original authors. Generalised haemangiomatosis of the skin and severe irreversibile cerebral damage are the essential symptoms of the disease. The assumed pathogenesis, morphological changes of the disease, and the therapeutical attempts are discussed.", "PMID": 979968}
{"id": "pubmed23n0032_12287", "title": "[Vegetative disorders in children with cerebral palsy. Results of an inquiry of parents].", "content": "Many of the disturbances resulting from dysregulations in the autonomous nervous system of children with cerebral palsy are rarely discussed in the doctor's praxis. Nevertheless, they are causes of trouble and worry for the parents. For this reason we started an inquiry into this matter. Questionnaires were sent to the parents of 452 C.P. patients. 374 were answered with sufficient care. The following factors were evaluated: sleep, bladder and bowel activity, temperature regulation, vomiting, sweating, blood circulation, growth. The C.P. children were compared to their own siblings especially to the next younger ones. The diagnoses were as follows: Spastic tetraplegia 197 patients. Spastic hemiplegia 44 patients, Athetosis 33 patients, Mixed cases of spasticity and athetosis 82 patients, Other 15 patients. The degrees of handicap in terms of motor development were: severe (unability to sit unsupported) 166 cases, moderate (unability to kneel or walk unsupported) 118 cases, mild (ability to kneel and/or walk unsupported) 87 cases. Summarized, the statements of the parents gave the following results: sleep disturbances: 169 cases (46%), constipation: 145 cases (39%), tendency towards temperature dysregulation: 112 cases (30%) , tendency towards increased vomiting: 91 cases (25%), sweating increased or decreased: 110 cases (30%), irregular and frequent voiding of bladder: 92 cases (25%), unstable regulation of blood circulation: 101 cases (27%), cold skin: 264 cases (71%), body-length deficit: 119 cases (32%), low-weight: 177 cases (48%), feet too small for age: 252 cases (68%). Results are related to diagnosis and severeness of handicap. In addition, it is discussed, whether there are relations between several of the investigated factors. The influence of the patients sex is discussed.", "contents": "[Vegetative disorders in children with cerebral palsy. Results of an inquiry of parents]. Many of the disturbances resulting from dysregulations in the autonomous nervous system of children with cerebral palsy are rarely discussed in the doctor's praxis. Nevertheless, they are causes of trouble and worry for the parents. For this reason we started an inquiry into this matter. Questionnaires were sent to the parents of 452 C.P. patients. 374 were answered with sufficient care. The following factors were evaluated: sleep, bladder and bowel activity, temperature regulation, vomiting, sweating, blood circulation, growth. The C.P. children were compared to their own siblings especially to the next younger ones. The diagnoses were as follows: Spastic tetraplegia 197 patients. Spastic hemiplegia 44 patients, Athetosis 33 patients, Mixed cases of spasticity and athetosis 82 patients, Other 15 patients. The degrees of handicap in terms of motor development were: severe (unability to sit unsupported) 166 cases, moderate (unability to kneel or walk unsupported) 118 cases, mild (ability to kneel and/or walk unsupported) 87 cases. Summarized, the statements of the parents gave the following results: sleep disturbances: 169 cases (46%), constipation: 145 cases (39%), tendency towards temperature dysregulation: 112 cases (30%) , tendency towards increased vomiting: 91 cases (25%), sweating increased or decreased: 110 cases (30%), irregular and frequent voiding of bladder: 92 cases (25%), unstable regulation of blood circulation: 101 cases (27%), cold skin: 264 cases (71%), body-length deficit: 119 cases (32%), low-weight: 177 cases (48%), feet too small for age: 252 cases (68%). Results are related to diagnosis and severeness of handicap. In addition, it is discussed, whether there are relations between several of the investigated factors. The influence of the patients sex is discussed.", "PMID": 979969}
{"id": "pubmed23n0032_12288", "title": "[Dysfunction of Granulocytes in Children with Frequent Infections].", "content": "Eight children with various diseases, who has frequent infections were examined for abnormalities of serum proteins and leucocyte function. Inborn or secondary defects could be recognized by the tests for chemotaxis, the intracellular killing of bacteria as well as yeast and the oxydative metabolism of the granulocytes. The defects were serum- or cell-dependent and they could be detected alone or in combination with other dysfunctions. Three children showed a reduced stimulation of the lymphocytes in addition to the impairment of chemotactic activity or fungicidal cavity.", "contents": "[Dysfunction of Granulocytes in Children with Frequent Infections]. Eight children with various diseases, who has frequent infections were examined for abnormalities of serum proteins and leucocyte function. Inborn or secondary defects could be recognized by the tests for chemotaxis, the intracellular killing of bacteria as well as yeast and the oxydative metabolism of the granulocytes. The defects were serum- or cell-dependent and they could be detected alone or in combination with other dysfunctions. Three children showed a reduced stimulation of the lymphocytes in addition to the impairment of chemotactic activity or fungicidal cavity.", "PMID": 979970}
{"id": "pubmed23n0032_12289", "title": "[Blood Pressure of Premature Infants within the First 3 Weeks of Life (author's transl)].", "content": "Technical difficulties which prevented routine blood pressure measurements in premature infants are nowadays overcome by the application of the ultrasound Doppler-technique. Normal values for premature infants with respect to birth weight and age are reported. They are needed for the detection of abnormal values and the therapy.", "contents": "[Blood Pressure of Premature Infants within the First 3 Weeks of Life (author's transl)]. Technical difficulties which prevented routine blood pressure measurements in premature infants are nowadays overcome by the application of the ultrasound Doppler-technique. Normal values for premature infants with respect to birth weight and age are reported. They are needed for the detection of abnormal values and the therapy.", "PMID": 979971}
{"id": "pubmed23n0032_12290", "title": "[On Endocrine Hypothalamic Systems (authors transl)].", "content": "The article summarizes under morphological aspects, the most important results of neuroendocrinology as far as hypothalamus and hypophysis are concerned.", "contents": "[On Endocrine Hypothalamic Systems (authors transl)]. The article summarizes under morphological aspects, the most important results of neuroendocrinology as far as hypothalamus and hypophysis are concerned.", "PMID": 979972}
{"id": "pubmed23n0032_12291", "title": "[The Restless Child (author's transl)].", "content": "Physiological unrest of children seems to originate from a basic need for activity in humans. It becomes apparent as \"enjoyment of action\" during age-specific periods, particularly during emotional phases of development. Apart from clearly neurological diseases, psychomotoric unrest as a pathological symptom can be observed in three forms different in artiology and psychodynamics: as cerebral unrest, psycho-vegetative unrest, and neurotic unrest. Causes, psychodynamics and appearances of the unrest as mentioned before are discussed and illustrated by details of case histories. Basically, the psychomotoric unrest is an expression of frustrated emotion, preventing the child from adjustment to reality, thereby causing a deficit of experience, which inturn is the reason for a secondary lack of success, to which the child reacts again with psychomotoric unrest. Therapy has to interrupt this vicious circle by three recommended approaches: drug therapy, child guidance, and psycho-therapy. Sociological aspects of the \"restless child\" are mentioned.", "contents": "[The Restless Child (author's transl)]. Physiological unrest of children seems to originate from a basic need for activity in humans. It becomes apparent as \"enjoyment of action\" during age-specific periods, particularly during emotional phases of development. Apart from clearly neurological diseases, psychomotoric unrest as a pathological symptom can be observed in three forms different in artiology and psychodynamics: as cerebral unrest, psycho-vegetative unrest, and neurotic unrest. Causes, psychodynamics and appearances of the unrest as mentioned before are discussed and illustrated by details of case histories. Basically, the psychomotoric unrest is an expression of frustrated emotion, preventing the child from adjustment to reality, thereby causing a deficit of experience, which inturn is the reason for a secondary lack of success, to which the child reacts again with psychomotoric unrest. Therapy has to interrupt this vicious circle by three recommended approaches: drug therapy, child guidance, and psycho-therapy. Sociological aspects of the \"restless child\" are mentioned.", "PMID": 979978}
{"id": "pubmed23n0032_12292", "title": "[Mass screening for histidinaemia:rationalization by selective thin layer chromatography. First results].", "content": "Since 1974 histidine and urocanic acid was estimated by thin layer chromatography (TLC) in all newborn infants in whom histidine blood levels had to be controlled because of an abnormal Guthrie-test. This entailed using dried blood spotted on filter paper to rationalize newborn screening for histidinaemia thus reducing the work involved. Three cases of histidinaemia were found amongst 66.064 newborn infants. In order to find these, 830 initialy suspicious Guthrie tests had to be checked. This is a control frequency of 1:80. 384 controls were necessitated by inhibition-zones, 482 by initialy elevated blood levels. Histidine and urocanic acid concentrations generally correlated well in the TLC; only 9 newborn infants showed results suggesting histidinaemia (elevated histidine and absent urocanic acid). All three histidinaemias discovered by Guthrie-test aswell were among them, so that in the end, with only 9 controls combining Guthrie-test and TLC, the same effectiveness could have been reached, as compared to 830, when using the Guthrie-test alone.", "contents": "[Mass screening for histidinaemia:rationalization by selective thin layer chromatography. First results]. Since 1974 histidine and urocanic acid was estimated by thin layer chromatography (TLC) in all newborn infants in whom histidine blood levels had to be controlled because of an abnormal Guthrie-test. This entailed using dried blood spotted on filter paper to rationalize newborn screening for histidinaemia thus reducing the work involved. Three cases of histidinaemia were found amongst 66.064 newborn infants. In order to find these, 830 initialy suspicious Guthrie tests had to be checked. This is a control frequency of 1:80. 384 controls were necessitated by inhibition-zones, 482 by initialy elevated blood levels. Histidine and urocanic acid concentrations generally correlated well in the TLC; only 9 newborn infants showed results suggesting histidinaemia (elevated histidine and absent urocanic acid). All three histidinaemias discovered by Guthrie-test aswell were among them, so that in the end, with only 9 controls combining Guthrie-test and TLC, the same effectiveness could have been reached, as compared to 830, when using the Guthrie-test alone.", "PMID": 979981}
{"id": "pubmed23n0032_12293", "title": "[About the further development of a 6 year old boy with essential hyperlipaemia (fat induced hypertriglyceridaemia)].", "content": "1961 the authors observed and described a rare form of Essential Hyperlipaemia seen in a 6 year old boy. On a diet poor of fat food the patient has been in good health without any complaints for the last 13 years. The disease did not show any tendency to recur. The child grew and lived through puberty quite normally. Only at the age of 19, during military service, the symptoms of the disease were noticed again. When released from the military service and put back to his usual diet his condition normalised quickly.", "contents": "[About the further development of a 6 year old boy with essential hyperlipaemia (fat induced hypertriglyceridaemia)]. 1961 the authors observed and described a rare form of Essential Hyperlipaemia seen in a 6 year old boy. On a diet poor of fat food the patient has been in good health without any complaints for the last 13 years. The disease did not show any tendency to recur. The child grew and lived through puberty quite normally. Only at the age of 19, during military service, the symptoms of the disease were noticed again. When released from the military service and put back to his usual diet his condition normalised quickly.", "PMID": 979982}
{"id": "pubmed23n0032_12294", "title": "[Spinocerebellar ataxia with monilethrix, typical facial features, and dental anomalies].", "content": "The authors present a form of heredodegenerative spinocerebellar ataxia with concomitant involvement of ectodermal structures, different from other variants. The disease was characterized by spinocerebellar ataxia (the complete form); curly, thin, short and dense hair, mikroskopically monilethrix; a characteristic face (hypertelorism, flattened and wide base of the nose, prominent nostrils, large mouth, thick lips); and abnormal teeth which were reduced in number and had gaps inbetween. The psycho intellectual development and the voice of the patient were normal. The first neurological symptomes were observed at the age of 3 years and progressively developed until now at the age of 6 years. Data necessary to establish the mode of hereditary transmission were not available.", "contents": "[Spinocerebellar ataxia with monilethrix, typical facial features, and dental anomalies]. The authors present a form of heredodegenerative spinocerebellar ataxia with concomitant involvement of ectodermal structures, different from other variants. The disease was characterized by spinocerebellar ataxia (the complete form); curly, thin, short and dense hair, mikroskopically monilethrix; a characteristic face (hypertelorism, flattened and wide base of the nose, prominent nostrils, large mouth, thick lips); and abnormal teeth which were reduced in number and had gaps inbetween. The psycho intellectual development and the voice of the patient were normal. The first neurological symptomes were observed at the age of 3 years and progressively developed until now at the age of 6 years. Data necessary to establish the mode of hereditary transmission were not available.", "PMID": 979983}
{"id": "pubmed23n0032_12295", "title": "[More recent aspects of treatment for hypothyroidism and goitre in children and adolescents (author's transl)].", "content": "According to more recent knowledge L-thyroxine alone is recommended for thyroid hormone replacement therapy, no longer the combination of L-thyroxine and triiodothyronin, since L-thyroxine is converted to triiodothyronine in the periphery of the body. Additional application of triiodothyronine causes unphysiological elevations of blood levels of triiodothyronine. When changing from the combined thyroid hormone therapy to treatment with L-thyroxine alone it is enough in most cases to replace the previous amount of L-thyroxine only; previous recommendations for the dosage of thyroid hormone were rather high. Average requirements of adults for L-thyroxine are around 170 mug per day. Children need about 90--100 mug per m2 body surface per day. -- When hypothyroidism is suspected in newborns or infants no protracted diagnostic procedures are justified with respect to brain development and its requirement of thyroid hormone during this period of life. Replacement therapy should be started within 1--2 days. The exact diagnosis can be established later, for instance during the third day of life when a temporary interuption of treatment for diagnostic purposes has no longer such negative effect on normal brain development. As long as physiological doses of L-thyroxine are used no side effects are to be expected for patients who later turn out to be euthyroid. -- Following exclusion of hyperthyroidism goitres in children and adolescents equally need thyroid hormone replacement therapy to reduce the size or prevent further enlargement or even the development of nodules within the gland. The dosage for this is about half to three quarters of the full replacement dose. The nature of thyroid nodules should be investigated, even surgically, since malignancies can develop in children and juveniles as well as in adults.", "contents": "[More recent aspects of treatment for hypothyroidism and goitre in children and adolescents (author's transl)]. According to more recent knowledge L-thyroxine alone is recommended for thyroid hormone replacement therapy, no longer the combination of L-thyroxine and triiodothyronin, since L-thyroxine is converted to triiodothyronine in the periphery of the body. Additional application of triiodothyronine causes unphysiological elevations of blood levels of triiodothyronine. When changing from the combined thyroid hormone therapy to treatment with L-thyroxine alone it is enough in most cases to replace the previous amount of L-thyroxine only; previous recommendations for the dosage of thyroid hormone were rather high. Average requirements of adults for L-thyroxine are around 170 mug per day. Children need about 90--100 mug per m2 body surface per day. -- When hypothyroidism is suspected in newborns or infants no protracted diagnostic procedures are justified with respect to brain development and its requirement of thyroid hormone during this period of life. Replacement therapy should be started within 1--2 days. The exact diagnosis can be established later, for instance during the third day of life when a temporary interuption of treatment for diagnostic purposes has no longer such negative effect on normal brain development. As long as physiological doses of L-thyroxine are used no side effects are to be expected for patients who later turn out to be euthyroid. -- Following exclusion of hyperthyroidism goitres in children and adolescents equally need thyroid hormone replacement therapy to reduce the size or prevent further enlargement or even the development of nodules within the gland. The dosage for this is about half to three quarters of the full replacement dose. The nature of thyroid nodules should be investigated, even surgically, since malignancies can develop in children and juveniles as well as in adults.", "PMID": 979989}
{"id": "pubmed23n0032_12296", "title": "[Leucine-sensitive hypoglycaemia and its treatment with diazoxide (author's transl)].", "content": "A seven months old infant suffered from convulsions due to leucine-sensitive hypoglycaemia. The convulsions could not be stopped by a diet lacking in leucine, but ceased after additional application of diazoxide; hypoglycaemia was no longer observed the mental development of the child is hoped to be normal.", "contents": "[Leucine-sensitive hypoglycaemia and its treatment with diazoxide (author's transl)]. A seven months old infant suffered from convulsions due to leucine-sensitive hypoglycaemia. The convulsions could not be stopped by a diet lacking in leucine, but ceased after additional application of diazoxide; hypoglycaemia was no longer observed the mental development of the child is hoped to be normal.", "PMID": 979990}
{"id": "pubmed23n0032_12297", "title": "L-Tryptophan administration in man: effects on gonadotrophin, growth hormone and cortisol secretion and on sexual motivation.", "content": "Long-lasting administration of L-tryptophan in neurologic patients caused a slight reduction of plasma luteinizing hormone levels without modifications of sexual motivation. Plasma follicle stimulating hormone concentrations were not altered by L-tryptophan. Short-lasting loading of L-tryptophan caused a statistically significant increase of plasma growth hormone levels, and a fall of plasma cortisol values in neurologic patients without neuroendocrine disorders. The pattern of plasma cortisol response to L-tryptophan loading was different in patients with neuroendocrine disorders. Plasma gonadotrophins seemed to be slightly reduced after the L-tryptophan loading.", "contents": "L-Tryptophan administration in man: effects on gonadotrophin, growth hormone and cortisol secretion and on sexual motivation. Long-lasting administration of L-tryptophan in neurologic patients caused a slight reduction of plasma luteinizing hormone levels without modifications of sexual motivation. Plasma follicle stimulating hormone concentrations were not altered by L-tryptophan. Short-lasting loading of L-tryptophan caused a statistically significant increase of plasma growth hormone levels, and a fall of plasma cortisol values in neurologic patients without neuroendocrine disorders. The pattern of plasma cortisol response to L-tryptophan loading was different in patients with neuroendocrine disorders. Plasma gonadotrophins seemed to be slightly reduced after the L-tryptophan loading.", "PMID": 979991}
{"id": "pubmed23n0032_12298", "title": "Monoamine oxidase defect in essential arterial hypertension. A single dose pargyline test.", "content": "A defect in the level of monoamine oxidase activity of platelets was observed in essential arterial hypertension. This defect seems dependent upon a lower rate of synthesis of the enzyme and not to the presence of an isoenzyme, as the net rate of return of the enzymatic activity after pargyline inhibition does not significantly differ between hypertensive and normotensive subjects.", "contents": "Monoamine oxidase defect in essential arterial hypertension. A single dose pargyline test. A defect in the level of monoamine oxidase activity of platelets was observed in essential arterial hypertension. This defect seems dependent upon a lower rate of synthesis of the enzyme and not to the presence of an isoenzyme, as the net rate of return of the enzymatic activity after pargyline inhibition does not significantly differ between hypertensive and normotensive subjects.", "PMID": 979992}
{"id": "pubmed23n0032_12299", "title": "A computer method for the kinetic analysis of enzyme activity.", "content": "A computer method is described in which an estimate of Km and V parameters is obtained by fitting a Michaelis-Menten hyperbola to experimental data. The frequency distribution of calculated parameters is far from normality. A statistical evaluation of the differences between kinetic parameters must be obtained by the use of non-parametric statistics.", "contents": "A computer method for the kinetic analysis of enzyme activity. A computer method is described in which an estimate of Km and V parameters is obtained by fitting a Michaelis-Menten hyperbola to experimental data. The frequency distribution of calculated parameters is far from normality. A statistical evaluation of the differences between kinetic parameters must be obtained by the use of non-parametric statistics.", "PMID": 979993}
{"id": "pubmed23n0032_12300", "title": "Plasma total and free tryptophan concentration in 'neurotic' and 'psychotic' depressive patients.", "content": "Plasma levels of total and free tryptophan were measured in patients with depressive neurosis and depressive psychosis, before and after recovery. No significant differences were found in total or free plasma tryptophan levels between 'neurotic' and 'psychotic' groups, and no change in these levels was found on recovery from depressive illness.", "contents": "Plasma total and free tryptophan concentration in 'neurotic' and 'psychotic' depressive patients. Plasma levels of total and free tryptophan were measured in patients with depressive neurosis and depressive psychosis, before and after recovery. No significant differences were found in total or free plasma tryptophan levels between 'neurotic' and 'psychotic' groups, and no change in these levels was found on recovery from depressive illness.", "PMID": 979996}
{"id": "pubmed23n0032_12301", "title": "Changes in serotonin metabolism during migraine attacks.", "content": "The concentration of 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA), kallikrein-like enzymes and beta-glucuronidase were quantified in the cerebrospinal fluid (CSF) during spontaneous migraine attacks. Plasma levels of kallikrein-like enzymes and beta-glucuronidase, as well as urinary levels of 5-HIAA as free acid and glucuronides were also measured. Correlation of these biochemical findings with various clinical variables showed that 5-HIAA in the CSF did not correlate with either the time sequences of migraine attacks or with clinical division of migraine into classical and common migraine. CSF 5-HIAA correlated positively with HVA, EEG photostimulation, the triggering of food and the therapeutic effect of the so-called antiserotonin treatment, and negatively with esterase activity. Urinary 5-HIAA showed a significant increase during the early headache stage.", "contents": "Changes in serotonin metabolism during migraine attacks. The concentration of 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA), kallikrein-like enzymes and beta-glucuronidase were quantified in the cerebrospinal fluid (CSF) during spontaneous migraine attacks. Plasma levels of kallikrein-like enzymes and beta-glucuronidase, as well as urinary levels of 5-HIAA as free acid and glucuronides were also measured. Correlation of these biochemical findings with various clinical variables showed that 5-HIAA in the CSF did not correlate with either the time sequences of migraine attacks or with clinical division of migraine into classical and common migraine. CSF 5-HIAA correlated positively with HVA, EEG photostimulation, the triggering of food and the therapeutic effect of the so-called antiserotonin treatment, and negatively with esterase activity. Urinary 5-HIAA showed a significant increase during the early headache stage.", "PMID": 979998}
{"id": "pubmed23n0032_12302", "title": "Clinical and animal pharmacology of migraine: new perspectives.", "content": "Animal and clinical pharmacological studies show in certain vascular beds a biphasic action [potentiation and inhibition of serotonin (5-HT) responses] of some anti-migraine drugs, such as ergotamine, methysergide and more recently Org GC 94. Potentiation occurs at therapeutic drug concentrations. The present investigations seem to support the 5-HT theory of migraine and other essential headaches. In this theory, anti-migraine drugs, such as ergotamine, methysergide, pizotifen, and Org GC 94 could reduce the occurrence of pain in migraine and other esscutial headaches by acting as partial agonists tending to correct a deficiency of central 5-HT concentrations or turnover.", "contents": "Clinical and animal pharmacology of migraine: new perspectives. Animal and clinical pharmacological studies show in certain vascular beds a biphasic action [potentiation and inhibition of serotonin (5-HT) responses] of some anti-migraine drugs, such as ergotamine, methysergide and more recently Org GC 94. Potentiation occurs at therapeutic drug concentrations. The present investigations seem to support the 5-HT theory of migraine and other essential headaches. In this theory, anti-migraine drugs, such as ergotamine, methysergide, pizotifen, and Org GC 94 could reduce the occurrence of pain in migraine and other esscutial headaches by acting as partial agonists tending to correct a deficiency of central 5-HT concentrations or turnover.", "PMID": 979997}
{"id": "pubmed23n0032_12303", "title": "Insulin secretion in migraine: influence on the blood levels of tryptophan.", "content": "The blood levels of free tryptophan, glucose and insulin were determined in different groups of patients suffering from headache, initially and after a glucose tolerance and a tolbutamide tolerance test. The control group consisted of subjects without neurological or psychiatric disturbance. A sharp drop of blood tryptophan levels was observed in migraine patients during the tolbutamide tolerance. Migraine attacks may be induced in certain subjects by a disturbance of 5-hydroxytryptamine turnover.", "contents": "Insulin secretion in migraine: influence on the blood levels of tryptophan. The blood levels of free tryptophan, glucose and insulin were determined in different groups of patients suffering from headache, initially and after a glucose tolerance and a tolbutamide tolerance test. The control group consisted of subjects without neurological or psychiatric disturbance. A sharp drop of blood tryptophan levels was observed in migraine patients during the tolbutamide tolerance. Migraine attacks may be induced in certain subjects by a disturbance of 5-hydroxytryptamine turnover.", "PMID": 979999}
{"id": "pubmed23n0032_12304", "title": "The content and manipulation of cognitive maps in children and adults.", "content": "Comparison of cognitive maps generated by first graders, fifth graders, and college students using a triangulation task revealed that the first graders possessed very accurate, coherent cognitive maps. However, analyses of the cognitive maps generated when Ss were asked to imagine either the room rotating (mental rotation) or themselves moving within the room (perspective-taking) revealed that the accuracy and completeness of the mental manipulation increased dramatically with age. Only the cognitive maps of the college students were coherent and accurate during mental rotation than during perspective-taking. Careful attention was given to the types of errors made, and several systematic error patterns were identified which reflected qualitative differences. These qualitative differences indicated mental manipulation was a two-stage process. In the first stage, a strategy of transformation was applied to the ordinal spatial relationships contained within the cognitive map. First graders evidenced difficulty here. In the second stage, more specific relationships between self and spatial layout were reconstructed. Fifth graders evidenced difficulty here. In contrast, college students were able to complete both stages of the process.", "contents": "The content and manipulation of cognitive maps in children and adults. Comparison of cognitive maps generated by first graders, fifth graders, and college students using a triangulation task revealed that the first graders possessed very accurate, coherent cognitive maps. However, analyses of the cognitive maps generated when Ss were asked to imagine either the room rotating (mental rotation) or themselves moving within the room (perspective-taking) revealed that the accuracy and completeness of the mental manipulation increased dramatically with age. Only the cognitive maps of the college students were coherent and accurate during mental rotation than during perspective-taking. Careful attention was given to the types of errors made, and several systematic error patterns were identified which reflected qualitative differences. These qualitative differences indicated mental manipulation was a two-stage process. In the first stage, a strategy of transformation was applied to the ordinal spatial relationships contained within the cognitive map. First graders evidenced difficulty here. In the second stage, more specific relationships between self and spatial layout were reconstructed. Fifth graders evidenced difficulty here. In contrast, college students were able to complete both stages of the process.", "PMID": 980000}
{"id": "pubmed23n0032_12305", "title": "Foam stability predictions of respiratory distress in infants delivered by repeat elective cesarean section.", "content": "Amniocentesis for assessment of lung maturity has been recommended before delivery by elective repeat cesarean section because respiratory distress may occur in infants delivered in this manner. To test this hypothesis, foam stability tests of amniotic fluid (84 cases) or gastric aspirate (25 cases) were used as interchangeable indexes of pulmonary maturity in 109 infants born by repeat elective cesarean section. Of the 81 infants with a positive foam stability test, only 2.5 per cent had transient respiratory distress, but 8.3 per cent with intermediate or negative foam stability tests had respiratory difficulty (P less than 0.025). The results support recommendations that fetal lung maturity should be assessed before elective repeat cesarean section to minimize the risk of respiratory morbidity.", "contents": "Foam stability predictions of respiratory distress in infants delivered by repeat elective cesarean section. Amniocentesis for assessment of lung maturity has been recommended before delivery by elective repeat cesarean section because respiratory distress may occur in infants delivered in this manner. To test this hypothesis, foam stability tests of amniotic fluid (84 cases) or gastric aspirate (25 cases) were used as interchangeable indexes of pulmonary maturity in 109 infants born by repeat elective cesarean section. Of the 81 infants with a positive foam stability test, only 2.5 per cent had transient respiratory distress, but 8.3 per cent with intermediate or negative foam stability tests had respiratory difficulty (P less than 0.025). The results support recommendations that fetal lung maturity should be assessed before elective repeat cesarean section to minimize the risk of respiratory morbidity.", "PMID": 980004}
{"id": "pubmed23n0032_12306", "title": "Risk factors associated with nasopharyngeal carcinoma.", "content": "We performed a case-control study of nasopharyngeal squamous-cell carcinoma to identify risk factors associated with the disease. Interviews with 156 patients and 267 controls revealed that increased risk was significantly associated with a prior history of ear, nose or throat disease (relative risk = 1.8, P = 0.006) and occupational exposure to fumes (relative risk = 2.0, P = 0.006), smoke (relative risk = 3.0, P = 0.008) and chemicals (relative risk = 2.4, P = 0.006). Among Chinese, other Asians and Mexican-Americans, increased risk was also associated with foreign birthplace (relative risk = 2.1, P = 0.04), probably reflecting childhood exposure to carcinogens in inhaled smoke. In Chinese patients the risk associated with foreign birthplace and occupational exposure increased among those with HLA-A2 and less than two antigens at the B locus, suggesting a genetic variation in susceptibility.", "contents": "Risk factors associated with nasopharyngeal carcinoma. We performed a case-control study of nasopharyngeal squamous-cell carcinoma to identify risk factors associated with the disease. Interviews with 156 patients and 267 controls revealed that increased risk was significantly associated with a prior history of ear, nose or throat disease (relative risk = 1.8, P = 0.006) and occupational exposure to fumes (relative risk = 2.0, P = 0.006), smoke (relative risk = 3.0, P = 0.008) and chemicals (relative risk = 2.4, P = 0.006). Among Chinese, other Asians and Mexican-Americans, increased risk was also associated with foreign birthplace (relative risk = 2.1, P = 0.04), probably reflecting childhood exposure to carcinogens in inhaled smoke. In Chinese patients the risk associated with foreign birthplace and occupational exposure increased among those with HLA-A2 and less than two antigens at the B locus, suggesting a genetic variation in susceptibility.", "PMID": 980005}
{"id": "pubmed23n0032_12307", "title": "The intrauterine device and deaths from spontaneous abortion.", "content": "To evaluate the intrauterine device as a risk factor for mortality associated with spontaneous abortion, we analyzed all deaths from spontaneous abortions reported in the period 1972-1974. Women dying from spontaneous abortions with a device in place were more likely to be young, white and married than those not wearing a device. Risk of death from spontaneous abortion was over 50 times greater for women who continued their pregnancy with a device in place than for those who did not. The Dalkon shield carried an increased risk of death, as compared to other devices, even after rates were adjusted for duration of use. However, pregnant women with either a loop or a coil in place also had a higher risk of dying from spontaneous abortion than those without any device. The results support the clinical recommendation that any device should be removed when pregnancy is first diagnosed.", "contents": "The intrauterine device and deaths from spontaneous abortion. To evaluate the intrauterine device as a risk factor for mortality associated with spontaneous abortion, we analyzed all deaths from spontaneous abortions reported in the period 1972-1974. Women dying from spontaneous abortions with a device in place were more likely to be young, white and married than those not wearing a device. Risk of death from spontaneous abortion was over 50 times greater for women who continued their pregnancy with a device in place than for those who did not. The Dalkon shield carried an increased risk of death, as compared to other devices, even after rates were adjusted for duration of use. However, pregnant women with either a loop or a coil in place also had a higher risk of dying from spontaneous abortion than those without any device. The results support the clinical recommendation that any device should be removed when pregnancy is first diagnosed.", "PMID": 980018}
{"id": "pubmed23n0032_12308", "title": "Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybridization.", "content": "The technic of DNA-DNA hybridization was used for prenatal diagnosis of a pregnancy at risk for homozygous alpha-thalassemia. Fibroblasts were cultured from amniotic fluid, and the number of alpha-globin genes in the DNA was quantified by hybridization with radioactive DNA complementary to alpha-globin mRNA sequences. As compared to control studies of DNA from patients with alpha-thalassemia syndromes and from unaffected subjects, the results indicated that the fetus had alpha-thalassemia-1. The diagnosis was confirmed by umbilical-cord blood studies.", "contents": "Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybridization. The technic of DNA-DNA hybridization was used for prenatal diagnosis of a pregnancy at risk for homozygous alpha-thalassemia. Fibroblasts were cultured from amniotic fluid, and the number of alpha-globin genes in the DNA was quantified by hybridization with radioactive DNA complementary to alpha-globin mRNA sequences. As compared to control studies of DNA from patients with alpha-thalassemia syndromes and from unaffected subjects, the results indicated that the fetus had alpha-thalassemia-1. The diagnosis was confirmed by umbilical-cord blood studies.", "PMID": 980019}
{"id": "pubmed23n0032_12309", "title": "Mechanism of increased renal clearnace of amylase/creatinine in acute pancreatitis.", "content": "We investigated three possible causes of the increased ratio of amylase/creatinine clearance observed in acute pancreatitis. The presence of rapidly cleared isoamylase was excluded by studies of serum and urine, which demonstrated no anomalous isoamylases. In pancreatitis, the ratios (+/-1 S.E.M.) of both pancreatic isoamylase (9.2+/-0.6 per cent) and salivary isoamylase (8.6+/-1.6 per cent) were significantly (P less than 0.01) elevated over respective control values (2.4+/-0.2 and 1.8+/-0.2 per cent). Increased glomerular permeability to amylase was excluded by the demonstration of normal renal clearance of dextrans. We tested tubular reabsorption of protein by measuring the renal clearance of beta2-microglobulin, which is relatively freely filtered at the glomerulus and then avidly reabsorbed by the normal tubule. During acute pancreatitis the ratio of the renal clearance of beta2-microglobulin to that of creatinine was 1.22+/-0.52 per cent, an 80-fold increase over normal (0.015+/-0.002 per cent), with a rapid return toward normal during convalescence. Presumably, this reversible renal tubular defect also reduces amylase reabsorption and accounts for the elevated renal clearance of amylase/creatinine observed in acute pancreatitis.", "contents": "Mechanism of increased renal clearnace of amylase/creatinine in acute pancreatitis. We investigated three possible causes of the increased ratio of amylase/creatinine clearance observed in acute pancreatitis. The presence of rapidly cleared isoamylase was excluded by studies of serum and urine, which demonstrated no anomalous isoamylases. In pancreatitis, the ratios (+/-1 S.E.M.) of both pancreatic isoamylase (9.2+/-0.6 per cent) and salivary isoamylase (8.6+/-1.6 per cent) were significantly (P less than 0.01) elevated over respective control values (2.4+/-0.2 and 1.8+/-0.2 per cent). Increased glomerular permeability to amylase was excluded by the demonstration of normal renal clearance of dextrans. We tested tubular reabsorption of protein by measuring the renal clearance of beta2-microglobulin, which is relatively freely filtered at the glomerulus and then avidly reabsorbed by the normal tubule. During acute pancreatitis the ratio of the renal clearance of beta2-microglobulin to that of creatinine was 1.22+/-0.52 per cent, an 80-fold increase over normal (0.015+/-0.002 per cent), with a rapid return toward normal during convalescence. Presumably, this reversible renal tubular defect also reduces amylase reabsorption and accounts for the elevated renal clearance of amylase/creatinine observed in acute pancreatitis.", "PMID": 980035}
{"id": "pubmed23n0032_12310", "title": "The Johns Hopkins experience in Columbia, Maryland.", "content": "A university-related prepaid group practice was established in Columbia, Maryland, in 1969, by the Johns Hopkins Medical Institutions with the cooperation of the Connecticut General Life Insurance Company. Objectives were based on the traditional triad of service, teaching and research. The effort continues. There has been visible success in the achievement of each of the objectives. It is evident, however, that the development of the Columbia Medical Plan would have been facilitated if there had been a larger population base in which to market membership in the Plan, if marketing had been under the direction of the health-care provider, and if the burden of the building and running of a hospital had not been assumed. Overall, the experience suggests that the Health Maintenance Organization movement has only modest ultimate potential as an option in health-care delivery in the current economic milieu of American medicine.", "contents": "The Johns Hopkins experience in Columbia, Maryland. A university-related prepaid group practice was established in Columbia, Maryland, in 1969, by the Johns Hopkins Medical Institutions with the cooperation of the Connecticut General Life Insurance Company. Objectives were based on the traditional triad of service, teaching and research. The effort continues. There has been visible success in the achievement of each of the objectives. It is evident, however, that the development of the Columbia Medical Plan would have been facilitated if there had been a larger population base in which to market membership in the Plan, if marketing had been under the direction of the health-care provider, and if the burden of the building and running of a hospital had not been assumed. Overall, the experience suggests that the Health Maintenance Organization movement has only modest ultimate potential as an option in health-care delivery in the current economic milieu of American medicine.", "PMID": 980036}
{"id": "pubmed23n0032_12311", "title": "Reciprocal relation between renin dependency and sodium dependency in essential hypertension.", "content": "To investigate the roles of angiotensin II and sodium in essential high-renin, normal-renin and low-renin hypertension, 14 patients received the competitive antagonist of angiotensin II, saralasin, during periods of sodium depletion and repletion. Blood-pressure response to saralasin was determined by the state of sodium balance. Patients from all three renin subgroups exhibited a fall in blood pressure when sufficiently sodium depleted, and an elevation in blood pressure when sodium replete or insufficiently depleted. However, those with low renin required loss of substantially more sodium (sufficient to elicit compensatory stimulation of renin) before depletion could be achieved. In patients with essential hypertension of all three renin subgroups, sodium balance determines the degree of participation of the renin-angiotensin system in sustaining high blood pressure. Even the low-renin type can become renin dependent with sufficient sodium depletion.", "contents": "Reciprocal relation between renin dependency and sodium dependency in essential hypertension. To investigate the roles of angiotensin II and sodium in essential high-renin, normal-renin and low-renin hypertension, 14 patients received the competitive antagonist of angiotensin II, saralasin, during periods of sodium depletion and repletion. Blood-pressure response to saralasin was determined by the state of sodium balance. Patients from all three renin subgroups exhibited a fall in blood pressure when sufficiently sodium depleted, and an elevation in blood pressure when sodium replete or insufficiently depleted. However, those with low renin required loss of substantially more sodium (sufficient to elicit compensatory stimulation of renin) before depletion could be achieved. In patients with essential hypertension of all three renin subgroups, sodium balance determines the degree of participation of the renin-angiotensin system in sustaining high blood pressure. Even the low-renin type can become renin dependent with sufficient sodium depletion.", "PMID": 980059}
{"id": "pubmed23n0032_12312", "title": "Platelet survival and morphology in homocystinuria due to cystathionine synthase deficiency.", "content": "In homocystinuria due to cystathionine synthase deficiency thromboembolism is a major cause of mortality and morbidity. Recent studies by others identified an abnormally shortened platelet survival and increased platelet vacuolization in patients with homocystinuria. When we studied six additional patients, however, we found the platelet survival to be within normal limits for each. The mean survival (+/-1 S.D.) was 9.75+/-0.94 days (normal, 9.27+/-1.06). In addition, platelets from five patients with homocystinuria and three obligate heterozygotes could not be distinguished from those of seven normal control subjects by electron microscopy. Specifically, no increased vacuolization was observed. Genetic heterogeneity, technical differences of differences in plasma homocystine concentrations could account for these descrepant results. The mechanism of thrombosis in homocystinuria remains an open question.", "contents": "Platelet survival and morphology in homocystinuria due to cystathionine synthase deficiency. In homocystinuria due to cystathionine synthase deficiency thromboembolism is a major cause of mortality and morbidity. Recent studies by others identified an abnormally shortened platelet survival and increased platelet vacuolization in patients with homocystinuria. When we studied six additional patients, however, we found the platelet survival to be within normal limits for each. The mean survival (+/-1 S.D.) was 9.75+/-0.94 days (normal, 9.27+/-1.06). In addition, platelets from five patients with homocystinuria and three obligate heterozygotes could not be distinguished from those of seven normal control subjects by electron microscopy. Specifically, no increased vacuolization was observed. Genetic heterogeneity, technical differences of differences in plasma homocystine concentrations could account for these descrepant results. The mechanism of thrombosis in homocystinuria remains an open question.", "PMID": 980060}
{"id": "pubmed23n0032_12313", "title": "Alimentary-tract ganglioneuromatosis. A major component of the syndrome of multiple endocrine neoplasia, type 2b.", "content": "Although the syndrome of multiple endocrine neoplasia, type 2b, is known to comprise mucosal ganglioneuromatosis, medullary thyroid carcinoma, pheochromocytoma and skeletal anomalies, we wish to document the importance of the alimentary-tract components. In nine patients with this syndrome, we found diffuse ganglioneurmatosis extending from the lips to the rectum. Alimentary symptoms, constipation or diarrhea or both were present in six of these patients. Similar symptoms were present in six of seven other affected patients from whom alimentary-tract tissue was not obtained. Five of the 16 patients had megacolon, which led to operation in four, and a sixth patient had colectomy for diverticulitis complicating diffuse diverticulosis. The alimentary manifestations warrant greater emphasis, not only because they were prominent in the patients whom we studied but also because in 14, these manifestations were present before the endocrine neoplasms were detected.", "contents": "Alimentary-tract ganglioneuromatosis. A major component of the syndrome of multiple endocrine neoplasia, type 2b. Although the syndrome of multiple endocrine neoplasia, type 2b, is known to comprise mucosal ganglioneuromatosis, medullary thyroid carcinoma, pheochromocytoma and skeletal anomalies, we wish to document the importance of the alimentary-tract components. In nine patients with this syndrome, we found diffuse ganglioneurmatosis extending from the lips to the rectum. Alimentary symptoms, constipation or diarrhea or both were present in six of these patients. Similar symptoms were present in six of seven other affected patients from whom alimentary-tract tissue was not obtained. Five of the 16 patients had megacolon, which led to operation in four, and a sixth patient had colectomy for diverticulitis complicating diffuse diverticulosis. The alimentary manifestations warrant greater emphasis, not only because they were prominent in the patients whom we studied but also because in 14, these manifestations were present before the endocrine neoplasms were detected.", "PMID": 980061}
{"id": "pubmed23n0032_12314", "title": "Enzyme replacement therapy for adenosine deaminase deficiency and severe combined immunodeficiency.", "content": "To evaluate their role as a form of replacement therapy, frozen irradiated red blood cells were administered to a child with adenosine deaminase deficiency associated with severe combined immunodeficiency disease. In vitro lymphocyte responses to mitogens and allogeneic cells were restored. Subsequently, a \"thymus shadow\" appeared, and immunoglobulin synthesis was demonstrated. Frozen irradiated plasma, which alone had no effect on lymphocytes numbers or responses, promoted lymphocytosis when given with frozen irradiated red blood cells. The patient received the transfusions with or without irradiated plasma at four-week intervals and remained free of infection for 17 months. The patient's lymphocyte adenosine triphosphate levels were elevated before therapy, which consistently reduced them without altering the lymphocyte adenosine deaminase activity. Enzyme replacement therapy may provide a way to treat patients with adenosine deaminase deficiency associated with severe combined immunodeficiency disease who do not have histocompatible bone-marrow donors.", "contents": "Enzyme replacement therapy for adenosine deaminase deficiency and severe combined immunodeficiency. To evaluate their role as a form of replacement therapy, frozen irradiated red blood cells were administered to a child with adenosine deaminase deficiency associated with severe combined immunodeficiency disease. In vitro lymphocyte responses to mitogens and allogeneic cells were restored. Subsequently, a \"thymus shadow\" appeared, and immunoglobulin synthesis was demonstrated. Frozen irradiated plasma, which alone had no effect on lymphocytes numbers or responses, promoted lymphocytosis when given with frozen irradiated red blood cells. The patient received the transfusions with or without irradiated plasma at four-week intervals and remained free of infection for 17 months. The patient's lymphocyte adenosine triphosphate levels were elevated before therapy, which consistently reduced them without altering the lymphocyte adenosine deaminase activity. Enzyme replacement therapy may provide a way to treat patients with adenosine deaminase deficiency associated with severe combined immunodeficiency disease who do not have histocompatible bone-marrow donors.", "PMID": 980079}
{"id": "pubmed23n0032_12315", "title": "Clinical syndrome of variant angina with normal coronary arteriogram.", "content": "We compared patients with variant angina (ST-segment elevation during pain) who had normal or near normal coronary arteriograms (Group 1) with 20 in whom variant angina occurred in the presence of obstructive coronary lesions (Group 2). A long history of nonexertional angina without angina of effort or previous infarction was the rule in Group 1, whereas recent-onset unstable angina preceded by effort angina and infarction predominated in Group 2 (P less than 0.001). Normal electrocardiograms at rest, with ischemic ST-segment elevation in the inferior leads, and ischemia-induced heart block and bradycardia, characterized Group 1, whereas abnormal electrocardiograms, ischemic involvement or fibrillation were more common in Group 2 (P less than 0.001). Variant angina with normal coronary arteriogram generally has a benign course and is probably unrelated to atherosclerosis.", "contents": "Clinical syndrome of variant angina with normal coronary arteriogram. We compared patients with variant angina (ST-segment elevation during pain) who had normal or near normal coronary arteriograms (Group 1) with 20 in whom variant angina occurred in the presence of obstructive coronary lesions (Group 2). A long history of nonexertional angina without angina of effort or previous infarction was the rule in Group 1, whereas recent-onset unstable angina preceded by effort angina and infarction predominated in Group 2 (P less than 0.001). Normal electrocardiograms at rest, with ischemic ST-segment elevation in the inferior leads, and ischemia-induced heart block and bradycardia, characterized Group 1, whereas abnormal electrocardiograms, ischemic involvement or fibrillation were more common in Group 2 (P less than 0.001). Variant angina with normal coronary arteriogram generally has a benign course and is probably unrelated to atherosclerosis.", "PMID": 980080}
{"id": "pubmed23n0032_12316", "title": "Treatment and prognosis of hemiballismus.", "content": "Acute hemiballismus due to a cerebrovascular lesion may have a grave prognosis. In the past nine years, we have treated 11 patients who had an acute onset of hemiballismus believed to be the result of an acute vascular lesion with neuroleptic drugs (most frequently haloperidol). None of the 11 died, and the movement disorders were greatly reduced or eliminated. In eight patients the drugs were withdrawn within six months, without recurrence of the movement disorders. Spinal-fluid homovanillic acid levels were increased in three patients, suggesting that altered dopaminergic feedback mechanisms may be involved in the pathophysiology of hemiballismus. Our observations suggest that the prognosis of hemiballismus is not necessarily as grave as has been believed, and that neuroleptic therapy may alter the outcome of this disorder.", "contents": "Treatment and prognosis of hemiballismus. Acute hemiballismus due to a cerebrovascular lesion may have a grave prognosis. In the past nine years, we have treated 11 patients who had an acute onset of hemiballismus believed to be the result of an acute vascular lesion with neuroleptic drugs (most frequently haloperidol). None of the 11 died, and the movement disorders were greatly reduced or eliminated. In eight patients the drugs were withdrawn within six months, without recurrence of the movement disorders. Spinal-fluid homovanillic acid levels were increased in three patients, suggesting that altered dopaminergic feedback mechanisms may be involved in the pathophysiology of hemiballismus. Our observations suggest that the prognosis of hemiballismus is not necessarily as grave as has been believed, and that neuroleptic therapy may alter the outcome of this disorder.", "PMID": 980081}
{"id": "pubmed23n0032_12317", "title": "Neutrophil kinetics in hereditary and congenital neutropenias.", "content": "Neutrophil production and distribution were studied in two families with autosomal dominantly inherited neutropenia to distinguish their illness from other neutropenic disorders. In addition to a reduced post-mitotic pool of bone-marrow neutrophils and neutrophil precursors, mitotic pool size was also reduced, ranging from 1.1 to 2.9 X 10(9) cells per kilogram (normal, 3.8 +/- 0.4 X 10(9) per kilogram). In vitro committed stem cells were reduced as well, ranging from 2 to 12/10(5) marrow cells (normal, 30 to 120/10(5)). In three patients neutrophil counts were observed to return to normal when they were adults. In one such subject studied, however, both the mitotic pool of neutrophil precursors and marrow committed stem cells were reduced. These abnormalities were in contrast to studies of a patient with chronic benign neutropenia of childhood and an infant with Kostmann-type congenital neutropenia, both of whom had increased marrow committed stem cells. These studies demonstrate several different mechanisms for production of neutropenia in these syndromes.", "contents": "Neutrophil kinetics in hereditary and congenital neutropenias. Neutrophil production and distribution were studied in two families with autosomal dominantly inherited neutropenia to distinguish their illness from other neutropenic disorders. In addition to a reduced post-mitotic pool of bone-marrow neutrophils and neutrophil precursors, mitotic pool size was also reduced, ranging from 1.1 to 2.9 X 10(9) cells per kilogram (normal, 3.8 +/- 0.4 X 10(9) per kilogram). In vitro committed stem cells were reduced as well, ranging from 2 to 12/10(5) marrow cells (normal, 30 to 120/10(5)). In three patients neutrophil counts were observed to return to normal when they were adults. In one such subject studied, however, both the mitotic pool of neutrophil precursors and marrow committed stem cells were reduced. These abnormalities were in contrast to studies of a patient with chronic benign neutropenia of childhood and an infant with Kostmann-type congenital neutropenia, both of whom had increased marrow committed stem cells. These studies demonstrate several different mechanisms for production of neutropenia in these syndromes.", "PMID": 980093}
{"id": "pubmed23n0032_12318", "title": "Detection of intrapulmonary hemorrhage with carbon monoxide uptake. Application in goodpasture's syndrome.", "content": "We devised a noninvasive measure of pulmonary hemorrhage of value in the management of Goodpasture's syndrome. We reasoned that alveolar uptake of inhaled carbon monoxide during breath holding would increase in the presence of extravascular blood, but clearance of its radioisotope (C15O) from a lung field would be delayed. Thus, the ratio of uptake to clearance would indicate lung hemorrhage. In 15 controls and six patients with renal failure without hemorrhage, this ratio ranged from 0.73 to 1.5. In eight patients with Goodpasture's syndrome the ratio ranged from 1.5 to 16.5, returning to normal between episodes  of bleeding. Measurements of carbon monoxide uptake alone in 10 patients with Goodpasture's syndrome were at times well above that predicted for their hemoglobin level, whereas in renal failure with acute pulmonary edema increased carbon monoxide uptake was rarely found. Thus, monitoring of the single-breath carbon monoxide uptake alone can detect episodes of lung hemorrhage.", "contents": "Detection of intrapulmonary hemorrhage with carbon monoxide uptake. Application in goodpasture's syndrome. We devised a noninvasive measure of pulmonary hemorrhage of value in the management of Goodpasture's syndrome. We reasoned that alveolar uptake of inhaled carbon monoxide during breath holding would increase in the presence of extravascular blood, but clearance of its radioisotope (C15O) from a lung field would be delayed. Thus, the ratio of uptake to clearance would indicate lung hemorrhage. In 15 controls and six patients with renal failure without hemorrhage, this ratio ranged from 0.73 to 1.5. In eight patients with Goodpasture's syndrome the ratio ranged from 1.5 to 16.5, returning to normal between episodes  of bleeding. Measurements of carbon monoxide uptake alone in 10 patients with Goodpasture's syndrome were at times well above that predicted for their hemoglobin level, whereas in renal failure with acute pulmonary edema increased carbon monoxide uptake was rarely found. Thus, monitoring of the single-breath carbon monoxide uptake alone can detect episodes of lung hemorrhage.", "PMID": 980094}
{"id": "pubmed23n0032_12319", "title": "Deaths from paracervical anesthesia used for first-trimester abortion, 1972-1975.", "content": "Since January 1, 1972, five women in the United States are reported to have died from paracervical anesthesia used for first-trimester abortions. This report describes the three most recent deaths. Toxic doses of lidocaine, substantiated by post-mortem blood levels of 5 and 9 mug per milliliter, probably led to two deaths; an intolerance to or inadvertent intravenous administration of mepivacaine probably caused the third. Administration of an appropriate dose of local anesthetic appears to be the single most important factor in preventing catastrophic reactions.", "contents": "Deaths from paracervical anesthesia used for first-trimester abortion, 1972-1975. Since January 1, 1972, five women in the United States are reported to have died from paracervical anesthesia used for first-trimester abortions. This report describes the three most recent deaths. Toxic doses of lidocaine, substantiated by post-mortem blood levels of 5 and 9 mug per milliliter, probably led to two deaths; an intolerance to or inadvertent intravenous administration of mepivacaine probably caused the third. Administration of an appropriate dose of local anesthetic appears to be the single most important factor in preventing catastrophic reactions.", "PMID": 980095}
{"id": "pubmed23n0032_12320", "title": "Nucleotide rigidity.", "content": "It is show that in aqueous solution the backbone conformation of adenosine is as much flexible as that of 3'-AMP, 5'-AMP and 3', 5'-ADP indicating that nucleotides are not any more rigid than nucleosides. The flexible conformation of the monomeric components is conserved in the nucleotidyl units of destacked ApA, ApApA and poly(A), but it is not conserved in base stacked conditions. The findings are extended to guanosine, uridine and cytidine systems. It is projected that in aqueous solution, conformations of the individual nucleotidyl units of yeast tRNAhe are confined to the classically stable domains in the base stacked region and non-rigid flexible structures populate in the unstacked region comprising D16, D17, G20, U47 and A76.", "contents": "Nucleotide rigidity. It is show that in aqueous solution the backbone conformation of adenosine is as much flexible as that of 3'-AMP, 5'-AMP and 3', 5'-ADP indicating that nucleotides are not any more rigid than nucleosides. The flexible conformation of the monomeric components is conserved in the nucleotidyl units of destacked ApA, ApApA and poly(A), but it is not conserved in base stacked conditions. The findings are extended to guanosine, uridine and cytidine systems. It is projected that in aqueous solution, conformations of the individual nucleotidyl units of yeast tRNAhe are confined to the classically stable domains in the base stacked region and non-rigid flexible structures populate in the unstacked region comprising D16, D17, G20, U47 and A76.", "PMID": 980104}
{"id": "pubmed23n0032_12321", "title": "Antibody response and protective ability of rabbits inoculated with Staphylococcus aureus strain CH-91.", "content": "Rabbits were inoculated subcutaneously with the protease-producing strain, CH-91, of Staphylococcus aureus of chicken origin. Dermatolysis was observed at the site of inoculation in them. Then the lesion outwardly healed up 5 to 6 weeks after the inoculation. When these recovered rabbits were reinoculated subcutaneously with the same number of viable cells as used in the first inoculation, none of them were affected with dermatolysis; that is, they had acquired a protective ability against an experimental challenge with viable cells. Antibodies against staphylococcal cells, alpha-hemolysin, and protease were detected in the serum of these rabbits 10 or 20 days after the first inoculation, but antibody against nuclease or beta-hemolysin was not. After the reinoculation, those antibodies showed a remarkable rise in most of the rabbits. These results suggest indirectly that the inoculated cells might have produced sufficient amounts of alpha-hemolysin and protease in the cutaneous tissue of rabbits. When rabbits were immunized with detoxicated culture supernatant of S. aureus strain CH-91, they exhibited antibody responses to alpha-hemolysin and protease mainly. Moreover, they were proved to have acquired a protective ability to an experimental challenge with viable cells when examined for the occurrence of dermatolysis as a marker of infection. On the contrary, when rabbits were immunized with killed cells, they presented a remarkable antibody response to staphylococcal cells. The immunized rabbits, however, acquired no protective ability.", "contents": "Antibody response and protective ability of rabbits inoculated with Staphylococcus aureus strain CH-91. Rabbits were inoculated subcutaneously with the protease-producing strain, CH-91, of Staphylococcus aureus of chicken origin. Dermatolysis was observed at the site of inoculation in them. Then the lesion outwardly healed up 5 to 6 weeks after the inoculation. When these recovered rabbits were reinoculated subcutaneously with the same number of viable cells as used in the first inoculation, none of them were affected with dermatolysis; that is, they had acquired a protective ability against an experimental challenge with viable cells. Antibodies against staphylococcal cells, alpha-hemolysin, and protease were detected in the serum of these rabbits 10 or 20 days after the first inoculation, but antibody against nuclease or beta-hemolysin was not. After the reinoculation, those antibodies showed a remarkable rise in most of the rabbits. These results suggest indirectly that the inoculated cells might have produced sufficient amounts of alpha-hemolysin and protease in the cutaneous tissue of rabbits. When rabbits were immunized with detoxicated culture supernatant of S. aureus strain CH-91, they exhibited antibody responses to alpha-hemolysin and protease mainly. Moreover, they were proved to have acquired a protective ability to an experimental challenge with viable cells when examined for the occurrence of dermatolysis as a marker of infection. On the contrary, when rabbits were immunized with killed cells, they presented a remarkable antibody response to staphylococcal cells. The immunized rabbits, however, acquired no protective ability.", "PMID": 980119}
{"id": "pubmed23n0032_12322", "title": "Accumulating process and concentration ratios of ingested blood meals in larvae and nymphs of ten species of ticks.", "content": "The accumulating process and concentration ratios of ingested blood meals in the larvae and nymphs of Amblyomma testudinarium, Haemaphysalis campanulata, H. concinna, H. formosensis, H. hystricis, H. kitaokai, the bisexual and parthenogenetic strains of H. longicornis, H. megaspinosa, and Ixodes persulcatus on rabbits and Argas japonicus on chickens were comparatively investigated. The larvae and nymphs of the ixodid ticks examined and the larvae of A. japonicus concentrated the ingested blood meals up to about 3 times. In general, the larvae showed low concentration ratios due to their tendency to imbibe a certain proportion of non-blood components. The nymphs generally showed high concentration ratios. H. kitaokai belongs to the subgenus Alloceraea, the members of which are unique rapid feeders. It showed a high activity in concentrating ingested blood meal and presented the same general pattern of feeding as the other ixodid ticks which are very slow feeders.", "contents": "Accumulating process and concentration ratios of ingested blood meals in larvae and nymphs of ten species of ticks. The accumulating process and concentration ratios of ingested blood meals in the larvae and nymphs of Amblyomma testudinarium, Haemaphysalis campanulata, H. concinna, H. formosensis, H. hystricis, H. kitaokai, the bisexual and parthenogenetic strains of H. longicornis, H. megaspinosa, and Ixodes persulcatus on rabbits and Argas japonicus on chickens were comparatively investigated. The larvae and nymphs of the ixodid ticks examined and the larvae of A. japonicus concentrated the ingested blood meals up to about 3 times. In general, the larvae showed low concentration ratios due to their tendency to imbibe a certain proportion of non-blood components. The nymphs generally showed high concentration ratios. H. kitaokai belongs to the subgenus Alloceraea, the members of which are unique rapid feeders. It showed a high activity in concentrating ingested blood meal and presented the same general pattern of feeding as the other ixodid ticks which are very slow feeders.", "PMID": 980120}
{"id": "pubmed23n0032_12323", "title": "Comparative observations on some bionomics of Japanese ixodid ticks under laboratory cultural conditions.", "content": "Seventeen species of Japanese ixodid ticks of the genera Amblyomma, Boophilus, Haemaphysalis, Ixodes, and Rhipicephalus were reared in the laboratory under almost the same cultural conditions. Their major biological characters, such as feeding, molting, oviposition, and hatching, were summarized in tables. There were some differences in these bionomics among the species. The general characteristics common to all the ticks, except Amblyomma testudinarium, were as follows. The feeding period in immature stages and adult females was 3 approximately 5 and 7 approximately 14 days, respectively. The engorged body weight in larvae, numphs, and females was 0.3 approximately 0.5, 3.5 approximately 5.0, and 100 approximately 300 mg, respectively. The potential sexuality could be estimated from the nymphal engorged weight in five species. The larval and nymphal molting periods at 25 degrees C were alike, or 10 approximately 20 days, and there were no significant sexual differences in nymphal molting period among six species. The body weight just after molting in nymphs, males, and females was 0.25 approximately 0.3, 1 approximately 2, and 2 approximately 3 mg, respectively. The pre-oviposition, oviposition, and egg-incubation periods at 25 degrees C were 4 approximately 10 days, 2 approximately 3 weeks, and 1 month, respectively.", "contents": "Comparative observations on some bionomics of Japanese ixodid ticks under laboratory cultural conditions. Seventeen species of Japanese ixodid ticks of the genera Amblyomma, Boophilus, Haemaphysalis, Ixodes, and Rhipicephalus were reared in the laboratory under almost the same cultural conditions. Their major biological characters, such as feeding, molting, oviposition, and hatching, were summarized in tables. There were some differences in these bionomics among the species. The general characteristics common to all the ticks, except Amblyomma testudinarium, were as follows. The feeding period in immature stages and adult females was 3 approximately 5 and 7 approximately 14 days, respectively. The engorged body weight in larvae, numphs, and females was 0.3 approximately 0.5, 3.5 approximately 5.0, and 100 approximately 300 mg, respectively. The potential sexuality could be estimated from the nymphal engorged weight in five species. The larval and nymphal molting periods at 25 degrees C were alike, or 10 approximately 20 days, and there were no significant sexual differences in nymphal molting period among six species. The body weight just after molting in nymphs, males, and females was 0.25 approximately 0.3, 1 approximately 2, and 2 approximately 3 mg, respectively. The pre-oviposition, oviposition, and egg-incubation periods at 25 degrees C were 4 approximately 10 days, 2 approximately 3 weeks, and 1 month, respectively.", "PMID": 980121}
{"id": "pubmed23n0032_12324", "title": "Nomograms for obtaining a necessary, minimum sample size. II. When distribution of proportions from binomial data is approximately normal.", "content": "Two different nomograms corresponding to finite and infinite populations were devised to obtain a necessary, minimum sample size at a 95% confidence rate when the distribution of sample proportions calculated from binomial data was approximately normal. They could also be used for obtaining the 95% confidence limits of the population proportion from a sample proportion after having carried out a work.", "contents": "Nomograms for obtaining a necessary, minimum sample size. II. When distribution of proportions from binomial data is approximately normal. Two different nomograms corresponding to finite and infinite populations were devised to obtain a necessary, minimum sample size at a 95% confidence rate when the distribution of sample proportions calculated from binomial data was approximately normal. They could also be used for obtaining the 95% confidence limits of the population proportion from a sample proportion after having carried out a work.", "PMID": 980122}
{"id": "pubmed23n0032_12325", "title": "[Evoked potentials of the somatosensory regions of the brains of healthy subjects].", "content": "Somatosensory evoked responses were recorded in 37 healthy adults, male and female, at the age of 18-53. These responses were polyphasic aperiodic and consisted of 7-9 waves. The early components of the response were more marked in monopolar recording. Differences in the fluctuation of the latent periods and amplitudes were found as dependent on the age. Ipsilateral responses contrary to the contralateral ones were more variable, had a less amplitude and frequency of appearance of separate components. The latent periods of ipsilateral responses were longer (3-7 ms) as compared to the contralateral ones.", "contents": "[Evoked potentials of the somatosensory regions of the brains of healthy subjects]. Somatosensory evoked responses were recorded in 37 healthy adults, male and female, at the age of 18-53. These responses were polyphasic aperiodic and consisted of 7-9 waves. The early components of the response were more marked in monopolar recording. Differences in the fluctuation of the latent periods and amplitudes were found as dependent on the age. Ipsilateral responses contrary to the contralateral ones were more variable, had a less amplitude and frequency of appearance of separate components. The latent periods of ipsilateral responses were longer (3-7 ms) as compared to the contralateral ones.", "PMID": 980164}
{"id": "pubmed23n0032_12326", "title": "[United neurons in the cat motor cortex].", "content": "Vertically oriented bundles of apical dendrites in the cat motor cortex are described by means of light and electron microscopy. Existence of desmoidal and dendro-dendritic contacts in the bundles is considered as the structural basis of neuronal electrotonic interaction within the limits of one column. Sliding axo-spinal contacts are described between the descending pyramidal axon in layer III and the pyramidal apical dentrite in layer V which, probably, serve for the purpose of regulating activity of the main cortical output elements.", "contents": "[United neurons in the cat motor cortex]. Vertically oriented bundles of apical dendrites in the cat motor cortex are described by means of light and electron microscopy. Existence of desmoidal and dendro-dendritic contacts in the bundles is considered as the structural basis of neuronal electrotonic interaction within the limits of one column. Sliding axo-spinal contacts are described between the descending pyramidal axon in layer III and the pyramidal apical dentrite in layer V which, probably, serve for the purpose of regulating activity of the main cortical output elements.", "PMID": 980165}
{"id": "pubmed23n0032_12327", "title": "[Effect of strychninization of the visual cortex on evoked activity in the rabbit lateral geniculate body].", "content": "Changes in responses of low-frequency and high-frequency LGB neurons were studied on anesthetized and paralyzed rabbits after strichninization of the visual cortex. It was found that in the group of low-frequency neurons the average discharge frequency of responses increased and latencies decreased indicating a dominance of corticofugal facilitation. On the contrary, in the group of high-frequency neurons the average discharge frequency of responses decreased and latencies increased. This suggests a dominance of corticofugal inhibition. The existence of reciprocal corticofugal influences on the neurons of different components of the visual pathways is assumed.", "contents": "[Effect of strychninization of the visual cortex on evoked activity in the rabbit lateral geniculate body]. Changes in responses of low-frequency and high-frequency LGB neurons were studied on anesthetized and paralyzed rabbits after strichninization of the visual cortex. It was found that in the group of low-frequency neurons the average discharge frequency of responses increased and latencies decreased indicating a dominance of corticofugal facilitation. On the contrary, in the group of high-frequency neurons the average discharge frequency of responses decreased and latencies increased. This suggests a dominance of corticofugal inhibition. The existence of reciprocal corticofugal influences on the neurons of different components of the visual pathways is assumed.", "PMID": 980166}
{"id": "pubmed23n0032_12328", "title": "[Reticular inhibition of evoked potentials of the ventro-basal thalamus and somatosensory cortex of the rat brain].", "content": "In rats immobilized with d-tubocurarine conditioning electrical stimulation (100/s, 300 ms) of the central grey matter, reticular formation of the midbrain and medulla depressed focal potentials in thalamic ventro-basal complex and somatosensory cortex evoked by electrical stimulation of the forelimb or medial meniscus. The average threshold current for conditioning stimulation of these structures was 70, 100 and 120 muA. A comparison of intensity and duration of evoked potentials depression (for two-fold threshold stimulation of the brain stem) is failed to detect any difference between the stimulated structures: immediately after conditioning stimulation the amplitude of the cortical evoked potentials and post-synaptic components of the evoked potentials in the ventro-basal complex were 50-60% less than control amplitude (p less than 0.01) the depression persisting for 0.5-1 s. The presynaptic component of the thalamic evoked potentials was depressed only after three five-fold threshold conditioning stimulation. The brain stem stimulation did not cause any facilitatory effect on focal potentials evoked by maximal and submaximal stimulation of the forelimb or medial lemniscus.", "contents": "[Reticular inhibition of evoked potentials of the ventro-basal thalamus and somatosensory cortex of the rat brain]. In rats immobilized with d-tubocurarine conditioning electrical stimulation (100/s, 300 ms) of the central grey matter, reticular formation of the midbrain and medulla depressed focal potentials in thalamic ventro-basal complex and somatosensory cortex evoked by electrical stimulation of the forelimb or medial meniscus. The average threshold current for conditioning stimulation of these structures was 70, 100 and 120 muA. A comparison of intensity and duration of evoked potentials depression (for two-fold threshold stimulation of the brain stem) is failed to detect any difference between the stimulated structures: immediately after conditioning stimulation the amplitude of the cortical evoked potentials and post-synaptic components of the evoked potentials in the ventro-basal complex were 50-60% less than control amplitude (p less than 0.01) the depression persisting for 0.5-1 s. The presynaptic component of the thalamic evoked potentials was depressed only after three five-fold threshold conditioning stimulation. The brain stem stimulation did not cause any facilitatory effect on focal potentials evoked by maximal and submaximal stimulation of the forelimb or medial lemniscus.", "PMID": 980167}
{"id": "pubmed23n0032_12329", "title": "[Properties of rhythmic discharge by cat motor cortex neurons evoked by a continuous current through their membranes].", "content": "The properties of rhythmic discharge of motor cortex neurons evoked by dc depolarizing current through the membrane were studied in acute experiments on immobilized cats. Linear relation between the rate of rhythmic discharge and the strength of current was revealed, the slope of this relation being 18-10.7 imp/s nA. Correlation between input resistance and the slope of the discharge frequency current curve was demonstrated. Two types of adaptation to current were observed: exponential and undamped oscillations. The relation of the latency of the first spike and first interspike interval to current was hyperbolic. The role of dendrites in the observed phenomena is discussed.", "contents": "[Properties of rhythmic discharge by cat motor cortex neurons evoked by a continuous current through their membranes]. The properties of rhythmic discharge of motor cortex neurons evoked by dc depolarizing current through the membrane were studied in acute experiments on immobilized cats. Linear relation between the rate of rhythmic discharge and the strength of current was revealed, the slope of this relation being 18-10.7 imp/s nA. Correlation between input resistance and the slope of the discharge frequency current curve was demonstrated. Two types of adaptation to current were observed: exponential and undamped oscillations. The relation of the latency of the first spike and first interspike interval to current was hyperbolic. The role of dendrites in the observed phenomena is discussed.", "PMID": 980168}
{"id": "pubmed23n0032_12330", "title": "[Electrophysiologic analysis of the functional organization of cortico-cerebellar connections].", "content": "The influence of associative (orbital-anterior, parietal) and projective (auditory, sensomotor) cerebral cortex areas stimulation on activity of the Purkinje neurons of cerebellar cortex was studied in adult cats under chloralose-nembutal or nembutal anesthesia. These reactions were compared with the responses to peripheral stimuli. Definite similarity in responses of the Purkinje cells to different cortical (associative, projective) stimuli was found both for types of neurons and their responsiveness. In responses of the Purkinje cells to peripheral stimulation there was no sharp similarity as it was in responses to cortical stimuli. So, in cortical stimulation almost similar number of neurons (above 50%) was excited and in peripheral stimulation the responsiveness of neurons had marked difference: to electrical stimulation of skin there were 44.6%, to auditory 34.2%, to visual 18.8% of neuron responses.", "contents": "[Electrophysiologic analysis of the functional organization of cortico-cerebellar connections]. The influence of associative (orbital-anterior, parietal) and projective (auditory, sensomotor) cerebral cortex areas stimulation on activity of the Purkinje neurons of cerebellar cortex was studied in adult cats under chloralose-nembutal or nembutal anesthesia. These reactions were compared with the responses to peripheral stimuli. Definite similarity in responses of the Purkinje cells to different cortical (associative, projective) stimuli was found both for types of neurons and their responsiveness. In responses of the Purkinje cells to peripheral stimulation there was no sharp similarity as it was in responses to cortical stimuli. So, in cortical stimulation almost similar number of neurons (above 50%) was excited and in peripheral stimulation the responsiveness of neurons had marked difference: to electrical stimulation of skin there were 44.6%, to auditory 34.2%, to visual 18.8% of neuron responses.", "PMID": 980169}
{"id": "pubmed23n0032_12331", "title": "[Electrotonic interaction of secondary neurons of the carp olfactory bulb].", "content": "Responses of the olfactory bulb secondary neurons provoked by olfactory tract electrical stimulation were studied in carp, using the intracellular recording technique. Spike responses in most neurons were identified as antidromic. Values of their latent periods ranged from 2.5 to 55 msec. In addition, two kinds of responses with a constant latent period were revealed: pseudoantidromic spikes and low-amplitude fast depolarizing potentials. It is supposed that both kinds of responses are generated by an antidromic spike of the neighbouring secondary neuron electrotonically coupled with the neuron recorded.", "contents": "[Electrotonic interaction of secondary neurons of the carp olfactory bulb]. Responses of the olfactory bulb secondary neurons provoked by olfactory tract electrical stimulation were studied in carp, using the intracellular recording technique. Spike responses in most neurons were identified as antidromic. Values of their latent periods ranged from 2.5 to 55 msec. In addition, two kinds of responses with a constant latent period were revealed: pseudoantidromic spikes and low-amplitude fast depolarizing potentials. It is supposed that both kinds of responses are generated by an antidromic spike of the neighbouring secondary neuron electrotonically coupled with the neuron recorded.", "PMID": 980170}
{"id": "pubmed23n0032_12332", "title": "[Reaction of caudate nucleus neurons to direct stimulation].", "content": "Extracellular recording from the caudate nucleus neurons were performed in experiments on immobilized cats during electrical stimulation of different parts of the same nucleus. Latencies varied from 3.5 to 40 msec. Most neurons could be excited by stimulation of the most rostral part of the caudate nucleus head. In most cases responses were independent of the stimulation place. The responses consisted of one or rarely two spike followed by inhibition of background activity. Then activity gradually restored without rebound. Afterdischarges were not observed. The caudate neurons hardly reproduced stimulating frequencies more than 30 per second and retained in most cases their own frequency level (on the average 5-15 spikes/sec.). The results are discussed in connection with participation of the caudate nucleus in the origin of spindle activity.", "contents": "[Reaction of caudate nucleus neurons to direct stimulation]. Extracellular recording from the caudate nucleus neurons were performed in experiments on immobilized cats during electrical stimulation of different parts of the same nucleus. Latencies varied from 3.5 to 40 msec. Most neurons could be excited by stimulation of the most rostral part of the caudate nucleus head. In most cases responses were independent of the stimulation place. The responses consisted of one or rarely two spike followed by inhibition of background activity. Then activity gradually restored without rebound. Afterdischarges were not observed. The caudate neurons hardly reproduced stimulating frequencies more than 30 per second and retained in most cases their own frequency level (on the average 5-15 spikes/sec.). The results are discussed in connection with participation of the caudate nucleus in the origin of spindle activity.", "PMID": 980171}
{"id": "pubmed23n0032_12333", "title": "[Electrophysiologic study of propagation of excitation in the cat pterygopalatine ganglion].", "content": "Action potentials were studied in nerves of the cat pterygopalatine ganglion evoked by stimuli applied to other nerves of the ganglion (in situ). It is established that most fibres passing through the ganglion are continuous sympathetic postganglionic fibres (not less than 3 groups). Most parasympathetic preganglionic fibres are synaptical on the ganglionic neurons and are represented by a group of fibres with the same threshold of excitation. Intracellular recording from single neurons of the pterygopalatine ganglion showed that stimulation of the Vidian nerve caused orthodromic spikes with short latency in one group of neurons and spikes with long latency in other neurons (2.5-6 ms and 10-40 ms respectively). Only fast fibres appear to terminate on most neurons of the ganglion, and only slow fibres do on some other neurons. Tonic activity was not observed when was performed from intact nerves of the pterygopalatine ganglion. The interacellular recording from single neurons of the ganglion showed that frequency of spike potentials either is low (1-3 per second) in some neurons or the potentials are absent in general in other neurons.", "contents": "[Electrophysiologic study of propagation of excitation in the cat pterygopalatine ganglion]. Action potentials were studied in nerves of the cat pterygopalatine ganglion evoked by stimuli applied to other nerves of the ganglion (in situ). It is established that most fibres passing through the ganglion are continuous sympathetic postganglionic fibres (not less than 3 groups). Most parasympathetic preganglionic fibres are synaptical on the ganglionic neurons and are represented by a group of fibres with the same threshold of excitation. Intracellular recording from single neurons of the pterygopalatine ganglion showed that stimulation of the Vidian nerve caused orthodromic spikes with short latency in one group of neurons and spikes with long latency in other neurons (2.5-6 ms and 10-40 ms respectively). Only fast fibres appear to terminate on most neurons of the ganglion, and only slow fibres do on some other neurons. Tonic activity was not observed when was performed from intact nerves of the pterygopalatine ganglion. The interacellular recording from single neurons of the ganglion showed that frequency of spike potentials either is low (1-3 per second) in some neurons or the potentials are absent in general in other neurons.", "PMID": 980172}
{"id": "pubmed23n0032_12334", "title": "[Types of locomotion in Ophiuroidea].", "content": "Locomotor movements of two species of ophiura from the Sea of Japan: Ophiura sarsi vadicola Djakonov and Amphipholis kochii L\u00fctken, were filmed. Ophiura sarsi moves by means of two symmetric pairs of arms which perform rhythmical rowing movements, the fifth arm is passive and directed backwards. Animals of this species do not use tube feet in locomotion. In Amphipholis kochii, three main modes of locomotor movements were found. (1) \"Breast stroke\" in which two symmetric side arms periodically move forwards, and then push forwards the body and three other arms. In this mode, one arm (leading) is directed forwards, while two others backwards. When the side arms move-forwards or backwards, a wave of successive flexions and extensions propagates along the arm segments. (2) \"Pushing\" by means of the hind arm and \"pulling\" with the help of the leading arm. (3) Locomotion by means of the tube feet which perform stepping movements. These three modes of locomotion are observed either separately or in various combinations with each other. In (1) and (2) modes of locomotion the tube feet perform also the coupling of the active arms with the ground which is necessary for the animal movement.", "contents": "[Types of locomotion in Ophiuroidea]. Locomotor movements of two species of ophiura from the Sea of Japan: Ophiura sarsi vadicola Djakonov and Amphipholis kochii L\u00fctken, were filmed. Ophiura sarsi moves by means of two symmetric pairs of arms which perform rhythmical rowing movements, the fifth arm is passive and directed backwards. Animals of this species do not use tube feet in locomotion. In Amphipholis kochii, three main modes of locomotor movements were found. (1) \"Breast stroke\" in which two symmetric side arms periodically move forwards, and then push forwards the body and three other arms. In this mode, one arm (leading) is directed forwards, while two others backwards. When the side arms move-forwards or backwards, a wave of successive flexions and extensions propagates along the arm segments. (2) \"Pushing\" by means of the hind arm and \"pulling\" with the help of the leading arm. (3) Locomotion by means of the tube feet which perform stepping movements. These three modes of locomotion are observed either separately or in various combinations with each other. In (1) and (2) modes of locomotion the tube feet perform also the coupling of the active arms with the ground which is necessary for the animal movement.", "PMID": 980173}
{"id": "pubmed23n0032_12335", "title": "[Coordination of arm movement during locomotion in Ophiuroidea].", "content": "Ophiura Amphipholis kochii L\u00fctken can move with any one of five arms directed forwards; therefore, each arm performs various functions during locomotion. The arm amputated together with the adjoining part of the central nervous ring is capable of complicated behaviour, particularly, of locomotion. Transection of the central nervous ring results in disturbances of the arms coordination. Experiments with amputation of a part of the arms showed that afferent signals from arms are important for determination of the leading arm and mode of locomotion. The experimental data suggest that each arm has an autonomous nervous centre, and coordinated activity of all arms is acheaved by an interaction of the nervous centres. The interaction results in function distribution among the arms and in synchronization of arm movements. The leading arm prevails, since the influences exerting by its centre determine activity of adjacent centres.", "contents": "[Coordination of arm movement during locomotion in Ophiuroidea]. Ophiura Amphipholis kochii L\u00fctken can move with any one of five arms directed forwards; therefore, each arm performs various functions during locomotion. The arm amputated together with the adjoining part of the central nervous ring is capable of complicated behaviour, particularly, of locomotion. Transection of the central nervous ring results in disturbances of the arms coordination. Experiments with amputation of a part of the arms showed that afferent signals from arms are important for determination of the leading arm and mode of locomotion. The experimental data suggest that each arm has an autonomous nervous centre, and coordinated activity of all arms is acheaved by an interaction of the nervous centres. The interaction results in function distribution among the arms and in synchronization of arm movements. The leading arm prevails, since the influences exerting by its centre determine activity of adjacent centres.", "PMID": 980174}
{"id": "pubmed23n0032_12336", "title": "[Postactivation inhibition of processes in interneurons transmitting the activity of lateral \"descending\" systems].", "content": "After-discharge depression of excitability is found in the interneurons specialized on transmission of activities from \"lateral\" descending systems. Many of these cells are propriospinal units sending axons to the lateral funicle (LF). Anti- und orthodromic spike potentials in these cells are followed by significant hyperpolarization. In some cells post-activation depression increased with a rise in intensity of LF stimulation that evoked antidromic discharge; inhibitory post-synaptic potentials were recorded in several units after LF stimulation subthreshold for evoking such discharges. Existance of inhibitory synaptic connections between neurons of the investigated group similar to recurrent connections in the motoneuronal pools is supposed.", "contents": "[Postactivation inhibition of processes in interneurons transmitting the activity of lateral \"descending\" systems]. After-discharge depression of excitability is found in the interneurons specialized on transmission of activities from \"lateral\" descending systems. Many of these cells are propriospinal units sending axons to the lateral funicle (LF). Anti- und orthodromic spike potentials in these cells are followed by significant hyperpolarization. In some cells post-activation depression increased with a rise in intensity of LF stimulation that evoked antidromic discharge; inhibitory post-synaptic potentials were recorded in several units after LF stimulation subthreshold for evoking such discharges. Existance of inhibitory synaptic connections between neurons of the investigated group similar to recurrent connections in the motoneuronal pools is supposed.", "PMID": 980175}
{"id": "pubmed23n0032_12337", "title": "Immune response to rauscher virus-induced leukemia in DBA mice. I. Role of cellular and humoral immunity in spontaneous regression.", "content": "DBA/1 and DBA/2 mice infected with the Rauscher leukemia virus developed a biphasic erythroleukemia. Transitory regression of the disease was closely associated with the appearance of tumor-specific antibodies and the exacerbation was preceded by the gradual decrease of antibody titer. The antibody-dependent cellular cytotoxicity could be detected earlier, than the complement-dependent cytotoxicity. Moreover, in each case the titer of antibody-dependent cellular cytotoxicity was higher than that of complement-dependent cytotoxicity. The results suggest that the antibody-dependent cellular cytotoxicity is mainly responsible for the rejection of tumor cells.", "contents": "Immune response to rauscher virus-induced leukemia in DBA mice. I. Role of cellular and humoral immunity in spontaneous regression. DBA/1 and DBA/2 mice infected with the Rauscher leukemia virus developed a biphasic erythroleukemia. Transitory regression of the disease was closely associated with the appearance of tumor-specific antibodies and the exacerbation was preceded by the gradual decrease of antibody titer. The antibody-dependent cellular cytotoxicity could be detected earlier, than the complement-dependent cytotoxicity. Moreover, in each case the titer of antibody-dependent cellular cytotoxicity was higher than that of complement-dependent cytotoxicity. The results suggest that the antibody-dependent cellular cytotoxicity is mainly responsible for the rejection of tumor cells.", "PMID": 980178}
{"id": "pubmed23n0032_12338", "title": "Lung colony assay in normal, irradiated and tumor bearing mice.", "content": "Lung colony assay performed in normal, X-ray whole body irradiated and tumor bearing mice showed that both irradiated and tumor bearing animals developed much more lung metastases than normal animals.", "contents": "Lung colony assay in normal, irradiated and tumor bearing mice. Lung colony assay performed in normal, X-ray whole body irradiated and tumor bearing mice showed that both irradiated and tumor bearing animals developed much more lung metastases than normal animals.", "PMID": 980179}
{"id": "pubmed23n0032_12339", "title": "Sacharose density gradient separation of Ehrlich ascites carcinoma and L-5178Y tumor cells in different cell cycle phases.", "content": "Optimal conditions were determined for the distribution of Ehrlich ascites carcinoma (EAC) and L-5178Y mouse tumor cells, proliferating in vivo, by their age within the cell cycle by sedimentation in a buffered linear sacharose density gradient. Measurements of cell size, DNA content and incorporation of tritiated thymidine in successive parts of the gradient confirmed the actual separation of cells of different age: in the upper fractions there were cells in G1 phase, in the middle fractions in S phase and in the lower layers of the gradient there were cells in G2 and/or M phase.", "contents": "Sacharose density gradient separation of Ehrlich ascites carcinoma and L-5178Y tumor cells in different cell cycle phases. Optimal conditions were determined for the distribution of Ehrlich ascites carcinoma (EAC) and L-5178Y mouse tumor cells, proliferating in vivo, by their age within the cell cycle by sedimentation in a buffered linear sacharose density gradient. Measurements of cell size, DNA content and incorporation of tritiated thymidine in successive parts of the gradient confirmed the actual separation of cells of different age: in the upper fractions there were cells in G1 phase, in the middle fractions in S phase and in the lower layers of the gradient there were cells in G2 and/or M phase.", "PMID": 980180}
{"id": "pubmed23n0032_12340", "title": "Excretion of Dische positive compounds in rats after irradiation and after administration of some cytostatic drugs.", "content": "In order to verify some pathophysiological aspects in radio- and chemotherapy of tumors the excretion of Dische positive compounds in rats was followed. During fractionated whole-body irradiation of animals it was found that up to the exposure with 50 R/day the organism is able to repair the disturbances provoked by irradiation for a certain time and that within the course of irradiation a permanently higher excretion of Dische positive compounds occurs. On the contrary, following the exposure of 100 R/day after a short increase continual decrease occurs lasting until the death of the animal. The excretion of Dische positive compounds in rats was followed also after the application of Cyclophosphamide, cis-dichlorodiammineplatinum (II) and Vinblastine. All these three cytostatic drugs produce an increased excretion of Dische positive compounds on the first day after the application. In the case of Cyclophosphamide, it was demonstrated that the excreted amount of Dische positive compounds was dose dependent.", "contents": "Excretion of Dische positive compounds in rats after irradiation and after administration of some cytostatic drugs. In order to verify some pathophysiological aspects in radio- and chemotherapy of tumors the excretion of Dische positive compounds in rats was followed. During fractionated whole-body irradiation of animals it was found that up to the exposure with 50 R/day the organism is able to repair the disturbances provoked by irradiation for a certain time and that within the course of irradiation a permanently higher excretion of Dische positive compounds occurs. On the contrary, following the exposure of 100 R/day after a short increase continual decrease occurs lasting until the death of the animal. The excretion of Dische positive compounds in rats was followed also after the application of Cyclophosphamide, cis-dichlorodiammineplatinum (II) and Vinblastine. All these three cytostatic drugs produce an increased excretion of Dische positive compounds on the first day after the application. In the case of Cyclophosphamide, it was demonstrated that the excreted amount of Dische positive compounds was dose dependent.", "PMID": 980181}
{"id": "pubmed23n0032_12341", "title": "Mechanism of development and morphology of secondary carcinomas of the oviducts in primary uterine corpus carcinoma.", "content": "The rate of incidence and morphology of metastases in 284 Fallopian tubes from 148 patients with primary uterine corpus cancer have been investigated. In 44 patients metastases were discovered in inner organs, of which in 17 (11.5% patients) in 23 oviducts. In 10 of these patients the Fallopian tubes were the only localization of metastases. Macroscopical alterations in the form of thickening and condensation, tumor nodules on the surface of the affected oviducts were discovered in 7 cases. In the remainder no macroscopical changes have been noted. There predominated lymphogenic metastases, in a smaller number of cases there were implantation and lymphogeno-implantation type metastases, localized mostly in the ampullary regions of the oviducts. More often, one could observe involvement by lymphogenic metastases of the subserosal layers together with muscular tissue, less frequently of mucosal layers, and total involvement of all layers of the wall of Fallopian tubes in the form of single or multicentric tumor nests, or diffuse tumor infiltration of tissues of the oviduct wall. Implantation metastases in the form of micronodules, multicellular agglomerates were discovered on the serosa more often than on the mucosa of Fallopian tubes. Secondary oviduct carcinomas differ from primary ones by their histological structure and character of growth. The Fallopian tubes play an essential role in the dissemination of metastases in the presence of primary uterine corpus carcinoma.", "contents": "Mechanism of development and morphology of secondary carcinomas of the oviducts in primary uterine corpus carcinoma. The rate of incidence and morphology of metastases in 284 Fallopian tubes from 148 patients with primary uterine corpus cancer have been investigated. In 44 patients metastases were discovered in inner organs, of which in 17 (11.5% patients) in 23 oviducts. In 10 of these patients the Fallopian tubes were the only localization of metastases. Macroscopical alterations in the form of thickening and condensation, tumor nodules on the surface of the affected oviducts were discovered in 7 cases. In the remainder no macroscopical changes have been noted. There predominated lymphogenic metastases, in a smaller number of cases there were implantation and lymphogeno-implantation type metastases, localized mostly in the ampullary regions of the oviducts. More often, one could observe involvement by lymphogenic metastases of the subserosal layers together with muscular tissue, less frequently of mucosal layers, and total involvement of all layers of the wall of Fallopian tubes in the form of single or multicentric tumor nests, or diffuse tumor infiltration of tissues of the oviduct wall. Implantation metastases in the form of micronodules, multicellular agglomerates were discovered on the serosa more often than on the mucosa of Fallopian tubes. Secondary oviduct carcinomas differ from primary ones by their histological structure and character of growth. The Fallopian tubes play an essential role in the dissemination of metastases in the presence of primary uterine corpus carcinoma.", "PMID": 980182}
{"id": "pubmed23n0032_12342", "title": "Hodgkin's lymphoma -- prognosis as related to histology, clinical stage and treatment.", "content": "The rapid advances in modern chemotherapy and actinotherapy of Hodgkin's lymphoma and an improvement in its diagnosis have ushered in numerous novel procedures. This is a preliminary analysis of the results of treatment of 117 patients with actinotherapy, polychemotherapy, consisting of a chemotherapeutic four-combination as substitution for MOPP and a combined actino-chemotherapy. Actinotherapy was found more efficient in localized disorders of stages I and II, while polychemotherapy proved the more suitable when the disease was generalized -- stages III and IV. The analysis of our results has brought only partial support to the prognostic significance of the histological classification of Hodgkin's lymphoma according to Rye. No difference of statistical significance has been noted between the histological types of lymphocyte dominance, nodular sclerosis and mixed cellularity. Lymphocyte depletion alone showed a conspicuously worse prognosis than the other histological types.", "contents": "Hodgkin's lymphoma -- prognosis as related to histology, clinical stage and treatment. The rapid advances in modern chemotherapy and actinotherapy of Hodgkin's lymphoma and an improvement in its diagnosis have ushered in numerous novel procedures. This is a preliminary analysis of the results of treatment of 117 patients with actinotherapy, polychemotherapy, consisting of a chemotherapeutic four-combination as substitution for MOPP and a combined actino-chemotherapy. Actinotherapy was found more efficient in localized disorders of stages I and II, while polychemotherapy proved the more suitable when the disease was generalized -- stages III and IV. The analysis of our results has brought only partial support to the prognostic significance of the histological classification of Hodgkin's lymphoma according to Rye. No difference of statistical significance has been noted between the histological types of lymphocyte dominance, nodular sclerosis and mixed cellularity. Lymphocyte depletion alone showed a conspicuously worse prognosis than the other histological types.", "PMID": 980183}
{"id": "pubmed23n0032_12343", "title": "Cerebral vasospasm after brain injury.", "content": "Severe vasospasm of the supraclinoidal portion of the internal carotid artery and the proximal part of the anterior and middle cerebral arteries was displayed by cerebral angiography in a 45 year old female patient who had developed progressive disturbance of consciousness and marked meningism with extremely bloody lumbar CSF within a few days after craniocerebral trauma. Aneurysm could be excluded angiographically and operatively. One and a half weeks after evacuation of a subdural haematoma and an intracerebral contusional bleeding, when the patient was discharged without any neurological deficit, control angiography showed that angiospasm had disappeared. The scanty literature on cerebral vasospasm after brain trauma is reviewed. The role of traumatic angiospasm in prognosis and the necessity for broad indications for cerebral angiography in cases with brain contusion is emphasized.", "contents": "Cerebral vasospasm after brain injury. Severe vasospasm of the supraclinoidal portion of the internal carotid artery and the proximal part of the anterior and middle cerebral arteries was displayed by cerebral angiography in a 45 year old female patient who had developed progressive disturbance of consciousness and marked meningism with extremely bloody lumbar CSF within a few days after craniocerebral trauma. Aneurysm could be excluded angiographically and operatively. One and a half weeks after evacuation of a subdural haematoma and an intracerebral contusional bleeding, when the patient was discharged without any neurological deficit, control angiography showed that angiospasm had disappeared. The scanty literature on cerebral vasospasm after brain trauma is reviewed. The role of traumatic angiospasm in prognosis and the necessity for broad indications for cerebral angiography in cases with brain contusion is emphasized.", "PMID": 980192}
{"id": "pubmed23n0032_12344", "title": "[Ultrasonic doppler technique for microvascular bypass (author's transl)].", "content": "Following an operative microvascular extraintracranial bypass, 50 patients were re-examined by the ultrasonic Doppler technique. The findings were compared with the results achieved by post-operative angiography. This examination of the patency of extraintracranial anastomoses with the ultrasonic Doppler technique can be conducted on an outpatient basis, is noninvasive and can be frequently repeated. It has a high degree of accuracy (96%). In addition, there were indications that, in certain areas, a gauge could be established for the function of the anastomosis by comparing the amplitude indices from the bypass and the afferent vessels. Key-words: Ultrasonic Doppler technique - microvascular bypass - cerebral blood flow - cerebrovascular disorders", "contents": "[Ultrasonic doppler technique for microvascular bypass (author's transl)]. Following an operative microvascular extraintracranial bypass, 50 patients were re-examined by the ultrasonic Doppler technique. The findings were compared with the results achieved by post-operative angiography. This examination of the patency of extraintracranial anastomoses with the ultrasonic Doppler technique can be conducted on an outpatient basis, is noninvasive and can be frequently repeated. It has a high degree of accuracy (96%). In addition, there were indications that, in certain areas, a gauge could be established for the function of the anastomosis by comparing the amplitude indices from the bypass and the afferent vessels. Key-words: Ultrasonic Doppler technique - microvascular bypass - cerebral blood flow - cerebrovascular disorders", "PMID": 980193}
{"id": "pubmed23n0032_12345", "title": "[False negative myelography in surgically confirmed lumbar intervertebral disk prolapse].", "content": "A consecutive series of 821 lumbar myelograms in patients operated on for prolapsed disks were analysed. 47 or 5-7% had to be listed false-negative in spite of excellent technical quality. To find out, whether anatomical variations or a special functional state of the subarachnoid space could have prevented a correct diagnosis, clinical symptoms and some special radiological parameters were examined and statistically evaluated: 1. Distances between peduncles of L4, L5, S1 2. Width of subarachnoid space in a coronal section of L4, L5, S1 3. Width of subarachnoid space in a sagittal section of L5 4. Distance between the posterior limit of the body of L5 and the anterior limit of the subarachnoid space. Measurement and statistical correlations did not reveal any correlation between false-negative myelograms and any of the anatomical variants measured. We still cannot rule out false-negative myelograms.", "contents": "[False negative myelography in surgically confirmed lumbar intervertebral disk prolapse]. A consecutive series of 821 lumbar myelograms in patients operated on for prolapsed disks were analysed. 47 or 5-7% had to be listed false-negative in spite of excellent technical quality. To find out, whether anatomical variations or a special functional state of the subarachnoid space could have prevented a correct diagnosis, clinical symptoms and some special radiological parameters were examined and statistically evaluated: 1. Distances between peduncles of L4, L5, S1 2. Width of subarachnoid space in a coronal section of L4, L5, S1 3. Width of subarachnoid space in a sagittal section of L5 4. Distance between the posterior limit of the body of L5 and the anterior limit of the subarachnoid space. Measurement and statistical correlations did not reveal any correlation between false-negative myelograms and any of the anatomical variants measured. We still cannot rule out false-negative myelograms.", "PMID": 980194}
{"id": "pubmed23n0032_12346", "title": "Lumbar spondylosis and narrow spinal canal.", "content": "Thirty four cases of spondylosis and narrow spinal canal were analysed and the possible mechanism of the syndrome of the narrow canal discussed. In spondylotic cases early operation is advisable.", "contents": "Lumbar spondylosis and narrow spinal canal. Thirty four cases of spondylosis and narrow spinal canal were analysed and the possible mechanism of the syndrome of the narrow canal discussed. In spondylotic cases early operation is advisable.", "PMID": 980195}
{"id": "pubmed23n0032_12347", "title": "Congenital intracerebral epithelial cysts.", "content": "The problem of intracerebral congenital epithelial cysts is discussed with reference to five clinical cases. On the basis of their morphological structure the author differentiates the intracerebral cyst with a ciliated, columnar epithelium from the ependymal cyst proper (more frequently located in the lateral recess of the medulla oblongata) and the posterior cerebellar cyst of meningeal arachnoid origin. The cyst with a ciliated columnar epithelium appears to be an ectopic intracerebral tissue, whose neuroepithelial or endodermic origin still remains to be demonstrated. This cyst has been commonly described as a colloid (epithelial) cyst of the 3rd ventricle but may also be localized at other points in the central nervous system. One of the cases studied was an epithelial cyst in the frontal lobe, without any connexion with the ventricle, and evolving as a cerebral tumour.", "contents": "Congenital intracerebral epithelial cysts. The problem of intracerebral congenital epithelial cysts is discussed with reference to five clinical cases. On the basis of their morphological structure the author differentiates the intracerebral cyst with a ciliated, columnar epithelium from the ependymal cyst proper (more frequently located in the lateral recess of the medulla oblongata) and the posterior cerebellar cyst of meningeal arachnoid origin. The cyst with a ciliated columnar epithelium appears to be an ectopic intracerebral tissue, whose neuroepithelial or endodermic origin still remains to be demonstrated. This cyst has been commonly described as a colloid (epithelial) cyst of the 3rd ventricle but may also be localized at other points in the central nervous system. One of the cases studied was an epithelial cyst in the frontal lobe, without any connexion with the ventricle, and evolving as a cerebral tumour.", "PMID": 980196}
{"id": "pubmed23n0032_12348", "title": "The tolosa-hunt syndrome: report of two cases.", "content": "The Tolosa-Hunt Syndrome consists of intermittent painful ophthalmoplegia resulting from a non-specific inflammatory process in the cavernous sinus and superior orbital fissure, which responds to steroid therapy. We report two additional cases to the literature of the Tolosa-Hunt Syndrome and we are interested in pointing out the importance of orbital venography in the diagnosis of this syndrome. Orbital venography demonstrates the partial occupation of the cavernous sinus and the lack of visualization of the 3rd segment of the superior ophthalmic vein.", "contents": "The tolosa-hunt syndrome: report of two cases. The Tolosa-Hunt Syndrome consists of intermittent painful ophthalmoplegia resulting from a non-specific inflammatory process in the cavernous sinus and superior orbital fissure, which responds to steroid therapy. We report two additional cases to the literature of the Tolosa-Hunt Syndrome and we are interested in pointing out the importance of orbital venography in the diagnosis of this syndrome. Orbital venography demonstrates the partial occupation of the cavernous sinus and the lack of visualization of the 3rd segment of the superior ophthalmic vein.", "PMID": 980197}
{"id": "pubmed23n0032_12349", "title": "A case of persistent hypoglossal artery.", "content": "A personal case of persistent hypoglossal artery is presented and the literature on the subject from 1967 to the present reviewed. The 16 cases collected, following on the 26 already reported in the well-known monograph of T.A. Lie on congenital anomalies of the carotid arteries, complete the statistical picture of this malformation. After discussing the embryonic origin and describing the radiological anatomy of the anomaly, the authors briefly present their case and discuss, in the light of the cases collected, the possible role of persistent hypoglossal artery in the pathogenesis of vascular diseases of the head.", "contents": "A case of persistent hypoglossal artery. A personal case of persistent hypoglossal artery is presented and the literature on the subject from 1967 to the present reviewed. The 16 cases collected, following on the 26 already reported in the well-known monograph of T.A. Lie on congenital anomalies of the carotid arteries, complete the statistical picture of this malformation. After discussing the embryonic origin and describing the radiological anatomy of the anomaly, the authors briefly present their case and discuss, in the light of the cases collected, the possible role of persistent hypoglossal artery in the pathogenesis of vascular diseases of the head.", "PMID": 980198}
{"id": "pubmed23n0032_12350", "title": "[Diagnostic value of cerebrospinal fluid oxyhemoglobin and bilirubin determination in cerebrovascular diseases].", "content": "The levels of oxyhaemoglobin and bilirubin were determined in the cerebrospinal fluid in 46 cases of subarachnoideal haemorrhage and cerebromeningeal haemorrhage, 18 cases of haemorrhagic infarction. Autopsy confirmation was available in 18 cases of haemorrhages and 18 cases of infarction. Determination of oxyhaemoglobin and bilirubin levels in the cerebrospinal fluid is not sufficient as a reliable basis for differentiating between cerebral haemorrhage and haemorrhagic infarction. High values of oxyhaemoglobin and bilirubin seem to indicate bleeding into the cerebrospinal fluid spaces. Assessment of oxyhaemoglobin level and cerebrospinal fluid sediment suggests in some cases the diagnosis.", "contents": "[Diagnostic value of cerebrospinal fluid oxyhemoglobin and bilirubin determination in cerebrovascular diseases]. The levels of oxyhaemoglobin and bilirubin were determined in the cerebrospinal fluid in 46 cases of subarachnoideal haemorrhage and cerebromeningeal haemorrhage, 18 cases of haemorrhagic infarction. Autopsy confirmation was available in 18 cases of haemorrhages and 18 cases of infarction. Determination of oxyhaemoglobin and bilirubin levels in the cerebrospinal fluid is not sufficient as a reliable basis for differentiating between cerebral haemorrhage and haemorrhagic infarction. High values of oxyhaemoglobin and bilirubin seem to indicate bleeding into the cerebrospinal fluid spaces. Assessment of oxyhaemoglobin level and cerebrospinal fluid sediment suggests in some cases the diagnosis.", "PMID": 980201}
{"id": "pubmed23n0032_12351", "title": "[Disappearance of blood from cerebrospinal fluid following subarachnoidal hemorrhage (preliminary note)].", "content": "On the basis of cerebrospinal fluid investigations in 17 patients with subarachnoideal haemorrhage admitted to the hospital in the time period from Nov. 1 1973 to March 15 1975 the authors observed that the blood disappears from the fluid usually 8 days after the onset and they suggest that control lumbar tap and angiography should be performed at that time for possible referral of patients for surgical treatment. From the 8th day on there is no need of administration of drugs increasing blood clotting.", "contents": "[Disappearance of blood from cerebrospinal fluid following subarachnoidal hemorrhage (preliminary note)]. On the basis of cerebrospinal fluid investigations in 17 patients with subarachnoideal haemorrhage admitted to the hospital in the time period from Nov. 1 1973 to March 15 1975 the authors observed that the blood disappears from the fluid usually 8 days after the onset and they suggest that control lumbar tap and angiography should be performed at that time for possible referral of patients for surgical treatment. From the 8th day on there is no need of administration of drugs increasing blood clotting.", "PMID": 980202}
{"id": "pubmed23n0032_12352", "title": "[Results of short and long-term treatment of parkinsonism with L-dopa].", "content": "The results of substitutive treatment of Parkinson's disease with L-dopa were assessed using a special chart for periodical examinations. This chart contains, among others, a 100-point scoring scale of parkinsonian disability which makes possible objective comparison of the condition of patients after various duration of treatment. The obtained results were compared according to age, sex, duration of disease, results of previous anticholinergic treatment. Ophthalmological examinations were carried out determining the width and reactions of pupils, accomodation and intraocular tension as well as side effects. The results of scoring demonstrated a high therapeutic effectiveness of L-dopa, lack of significant ophthalmological abnormalities and mild degree of side effects due to peripheral and autonomic-system reactions to L-dopa.", "contents": "[Results of short and long-term treatment of parkinsonism with L-dopa]. The results of substitutive treatment of Parkinson's disease with L-dopa were assessed using a special chart for periodical examinations. This chart contains, among others, a 100-point scoring scale of parkinsonian disability which makes possible objective comparison of the condition of patients after various duration of treatment. The obtained results were compared according to age, sex, duration of disease, results of previous anticholinergic treatment. Ophthalmological examinations were carried out determining the width and reactions of pupils, accomodation and intraocular tension as well as side effects. The results of scoring demonstrated a high therapeutic effectiveness of L-dopa, lack of significant ophthalmological abnormalities and mild degree of side effects due to peripheral and autonomic-system reactions to L-dopa.", "PMID": 980203}
{"id": "pubmed23n0032_12353", "title": "[Effect of diphynylhydantoin on the histological appearance of internal organs in cat (preliminary study)].", "content": "The effects of DPH on internal organs of cats were investigated. The drug (Hydantoinal POLFA) was administered orally in doses 8 to 20 mg/kg daily during from 27 to 560 days. The initial dose was 1.5 mg/kg and it was increased gradually. Consistently occurring changes were found in the liver, kidneys, salivary glands and spleen. In the liver and kidneys the character of changes was mainly degenerative (fatty infiltration of hepatocytes and renal tubular epithelial cells). In the salivary gland the number of mucus-producing cells was increased, in the spleen proliferation of connective tissue and congestion were present.", "contents": "[Effect of diphynylhydantoin on the histological appearance of internal organs in cat (preliminary study)]. The effects of DPH on internal organs of cats were investigated. The drug (Hydantoinal POLFA) was administered orally in doses 8 to 20 mg/kg daily during from 27 to 560 days. The initial dose was 1.5 mg/kg and it was increased gradually. Consistently occurring changes were found in the liver, kidneys, salivary glands and spleen. In the liver and kidneys the character of changes was mainly degenerative (fatty infiltration of hepatocytes and renal tubular epithelial cells). In the salivary gland the number of mucus-producing cells was increased, in the spleen proliferation of connective tissue and congestion were present.", "PMID": 980204}
{"id": "pubmed23n0032_12354", "title": "[Nervous system diseases in workers of a large metallurgic plant].", "content": "Epidemiological investigations were carried out in an industrial plant in a population of about 16 000 people. The overall morbidity was 1 241.8 cases of nervous system diseases per 100 000 of population and the annual prevalence of neurological diseases was 473.7 per 100 000. The most frequent disease was sciatic pain, followed in order of frequency by epilepsy, vasomotor headaches, subjective symptoms after craniocerebral trauma, Parkinson's disease, clinically evident cerebral atherosclerosis and disseminated sclerosis. No significant effect of the type of occupation on the development of nervous system diseases was observed.", "contents": "[Nervous system diseases in workers of a large metallurgic plant]. Epidemiological investigations were carried out in an industrial plant in a population of about 16 000 people. The overall morbidity was 1 241.8 cases of nervous system diseases per 100 000 of population and the annual prevalence of neurological diseases was 473.7 per 100 000. The most frequent disease was sciatic pain, followed in order of frequency by epilepsy, vasomotor headaches, subjective symptoms after craniocerebral trauma, Parkinson's disease, clinically evident cerebral atherosclerosis and disseminated sclerosis. No significant effect of the type of occupation on the development of nervous system diseases was observed.", "PMID": 980200}
{"id": "pubmed23n0032_12355", "title": "[Cerebrospinal fluid lactates following craniocerebral injuries].", "content": "CSF lactate concentration was determined in 37 patients after craniocerebral injury. In 26 cases the lactate concentration was raised above the normal value (15 mg/100 ml) and in 11 cases it was above 30 mg/100 ml. All fatal cases were in the group with lactate level above 30 mg/100 ml. The study confirms the fact that CSF lactate concentration is related to the severity of cerebral tissue damage and has a prognostic significance.", "contents": "[Cerebrospinal fluid lactates following craniocerebral injuries]. CSF lactate concentration was determined in 37 patients after craniocerebral injury. In 26 cases the lactate concentration was raised above the normal value (15 mg/100 ml) and in 11 cases it was above 30 mg/100 ml. All fatal cases were in the group with lactate level above 30 mg/100 ml. The study confirms the fact that CSF lactate concentration is related to the severity of cerebral tissue damage and has a prognostic significance.", "PMID": 980205}
{"id": "pubmed23n0032_12356", "title": "[Statistical correlation between lactate concentration and acid-base equilibrium in cases of brain tumors, craniocerebral injuries and meningitis].", "content": "Statistical significance of correlations between lactate concentration and pH value as well as pCO2 in the cerebrospinal fluid was assessed in patients with brain tumours, craniocerebral injury and meningitis. After calculation of correlation indices it was found that with increasing lactate level the correlation between this level and the pH and pCO2 of the cerebrospinal fluid increases. Correlation coefficients were far from their maximal values (+1 or -1) which indicates absence of a direct cause-and-effect relationship between lactate concentration and both remaining values. The analysis of results in the group with lactate level exceeding 30 mg/100 ml demonstrated lowest correlation coefficients between these values in patients with meningoencephalitis.", "contents": "[Statistical correlation between lactate concentration and acid-base equilibrium in cases of brain tumors, craniocerebral injuries and meningitis]. Statistical significance of correlations between lactate concentration and pH value as well as pCO2 in the cerebrospinal fluid was assessed in patients with brain tumours, craniocerebral injury and meningitis. After calculation of correlation indices it was found that with increasing lactate level the correlation between this level and the pH and pCO2 of the cerebrospinal fluid increases. Correlation coefficients were far from their maximal values (+1 or -1) which indicates absence of a direct cause-and-effect relationship between lactate concentration and both remaining values. The analysis of results in the group with lactate level exceeding 30 mg/100 ml demonstrated lowest correlation coefficients between these values in patients with meningoencephalitis.", "PMID": 980206}
{"id": "pubmed23n0032_12357", "title": "[Traumatic arteriovenous fistula of the middle meningeal artery closed spontaneously].", "content": "The authors describe a case of traumatic arteriovenous fistula developing between the branches of the middle meningeal artery and the meningeal and intraosseous veins. The fistula closed spontaneously.", "contents": "[Traumatic arteriovenous fistula of the middle meningeal artery closed spontaneously]. The authors describe a case of traumatic arteriovenous fistula developing between the branches of the middle meningeal artery and the meningeal and intraosseous veins. The fistula closed spontaneously.", "PMID": 980207}
{"id": "pubmed23n0032_12358", "title": "[Subdural empyema as a complication of maxillary sinusitis (description of 3 cases)].", "content": "The authors report 3 cases of subdural empyema with different course and outcome. In all of them subdural empyema developed as a complication of chronic maxillary sinusitis. In the first case a coexistent brain abscess was the cause of death. In the second case operation carried out when the patient was in extremely severe condition failed to prevent death since the diagnosis had been made too late. Complete recovery was obtained in the third case owing to early clinical diagnosis confirmed with angiography, followed by early surgical treatment of the primary focus and early craniotomy. The importance of early diagnosis is stressed and the methods of surgical treatment are discussed.", "contents": "[Subdural empyema as a complication of maxillary sinusitis (description of 3 cases)]. The authors report 3 cases of subdural empyema with different course and outcome. In all of them subdural empyema developed as a complication of chronic maxillary sinusitis. In the first case a coexistent brain abscess was the cause of death. In the second case operation carried out when the patient was in extremely severe condition failed to prevent death since the diagnosis had been made too late. Complete recovery was obtained in the third case owing to early clinical diagnosis confirmed with angiography, followed by early surgical treatment of the primary focus and early craniotomy. The importance of early diagnosis is stressed and the methods of surgical treatment are discussed.", "PMID": 980208}
{"id": "pubmed23n0032_12359", "title": "[Case of Hakim's syndrome].", "content": "The authors report a case of Hakim's syndrome developing in a 53-year-old man. Manifestations of parkinsonian syndrome prevailed. After implantation of a Pudenz-type valve significant improvement was obtained which confirms the clinical diagnosis.", "contents": "[Case of Hakim's syndrome]. The authors report a case of Hakim's syndrome developing in a 53-year-old man. Manifestations of parkinsonian syndrome prevailed. After implantation of a Pudenz-type valve significant improvement was obtained which confirms the clinical diagnosis.", "PMID": 980209}
{"id": "pubmed23n0032_12360", "title": "[Differential diagnosis of the level of traumatic damage of cervical spinal nerves and brachial plexus].", "content": "In 80 patients with paresis or paralysis of the upper extremity due to injury to the brachial plexus or cervical roots, EMG with muscular test, skin thermometry and radiculography were done. The comparison of EMG investigations and Lovett's test with the diagram of segmental and peripheral innervation gave only an approximate determination of the level and extent of injury. Skin thermometry demonstrated vasomot\u00f3r disturbances in 330 patients. Vasodilating activity was present, in the first place, in patients with complete paralysis of the extremity. These cases showed no significant improvement after rehabilitation management. Radiculography permitted to determine exactly the number of damaged spinal nerve pouches and this damage was found in 16 out of 18 patients. The character and location of pain in the upper extremity were without any significant diagnostic value in relation to the site and degree of injury.", "contents": "[Differential diagnosis of the level of traumatic damage of cervical spinal nerves and brachial plexus]. In 80 patients with paresis or paralysis of the upper extremity due to injury to the brachial plexus or cervical roots, EMG with muscular test, skin thermometry and radiculography were done. The comparison of EMG investigations and Lovett's test with the diagram of segmental and peripheral innervation gave only an approximate determination of the level and extent of injury. Skin thermometry demonstrated vasomot\u00f3r disturbances in 330 patients. Vasodilating activity was present, in the first place, in patients with complete paralysis of the extremity. These cases showed no significant improvement after rehabilitation management. Radiculography permitted to determine exactly the number of damaged spinal nerve pouches and this damage was found in 16 out of 18 patients. The character and location of pain in the upper extremity were without any significant diagnostic value in relation to the site and degree of injury.", "PMID": 980211}
{"id": "pubmed23n0032_12361", "title": "[Combined surgical and physical treatment in traumatic painful syndromes of the cervical spine].", "content": "Clinical observations suggest the need for changing therapeutic management to a more active one in cases of cervical spine injury with damage to the spinal cord and nerve roots or brachial plexus. In 248 patients with these injuries treated initially conservatively the incidence of cervicobrachial pain was analysed. Neuralgic pains were present in 31.5% of cases, causalgic pains in 2.4% and sympathalgic pains in 2%. Conservative treatment conducted in these patients (89 cases) during many months after trauma had no effect on return of mobility. Long-term application of physioterapy prevented only temporarily the development of trophic changes and only partially relieved pains. Only surgical decompression of the spinal cord or spinal nerves with stabilization of damaged vertebrae caused disappearance of painful syndromes and improvement in the motor activity of the extremities. These observations show that early surgical intervention for decompression of the spinal cord, roots or brachial plexus should be advocated in these cases.", "contents": "[Combined surgical and physical treatment in traumatic painful syndromes of the cervical spine]. Clinical observations suggest the need for changing therapeutic management to a more active one in cases of cervical spine injury with damage to the spinal cord and nerve roots or brachial plexus. In 248 patients with these injuries treated initially conservatively the incidence of cervicobrachial pain was analysed. Neuralgic pains were present in 31.5% of cases, causalgic pains in 2.4% and sympathalgic pains in 2%. Conservative treatment conducted in these patients (89 cases) during many months after trauma had no effect on return of mobility. Long-term application of physioterapy prevented only temporarily the development of trophic changes and only partially relieved pains. Only surgical decompression of the spinal cord or spinal nerves with stabilization of damaged vertebrae caused disappearance of painful syndromes and improvement in the motor activity of the extremities. These observations show that early surgical intervention for decompression of the spinal cord, roots or brachial plexus should be advocated in these cases.", "PMID": 980212}
{"id": "pubmed23n0032_12362", "title": "[Pathomechanism of pain in vertebral column diseases].", "content": "The pathological mechanism of pain in vertebral column diseases is discussed in accordance with the theory of Melzack and Wall taking into account both systems of pain sensation control at the spinal level and reticular formation level. The action of pain mediators is discussed as well. The main pathogenetic factors are: compression, stretching and ischaemia. Other factors, functioning particularly at the cervical spine level are, most frequently, developmental anomalies in the occipito-cervical area and faulty posture assumed during occupational work, as well as injuries which may be remote in time and forgotten. Elimination of pain perception may be obtained with surgical methods or by physicochemical means blocking the conduction of pain impulses.", "contents": "[Pathomechanism of pain in vertebral column diseases]. The pathological mechanism of pain in vertebral column diseases is discussed in accordance with the theory of Melzack and Wall taking into account both systems of pain sensation control at the spinal level and reticular formation level. The action of pain mediators is discussed as well. The main pathogenetic factors are: compression, stretching and ischaemia. Other factors, functioning particularly at the cervical spine level are, most frequently, developmental anomalies in the occipito-cervical area and faulty posture assumed during occupational work, as well as injuries which may be remote in time and forgotten. Elimination of pain perception may be obtained with surgical methods or by physicochemical means blocking the conduction of pain impulses.", "PMID": 980210}
{"id": "pubmed23n0032_12363", "title": "[Combined pharmacological and traction treatment in cases of painful syndromes of the cervical spine with degenerative and deformative changes].", "content": "In a group of 35 patients with cervical painful syndromes due to degenerative changes the authors applied traction treatment together with pharmacological agents. The patients were chosen because of long-standing symptoms, frequent recurrences or poor therapeutic effects. Using Glisson's loop for traction applied to the cervical spine the authors obtained good results, i.e. shortening of the treatment time, and prolongation of improvement. The most frequent complication of this method was vertigo.", "contents": "[Combined pharmacological and traction treatment in cases of painful syndromes of the cervical spine with degenerative and deformative changes]. In a group of 35 patients with cervical painful syndromes due to degenerative changes the authors applied traction treatment together with pharmacological agents. The patients were chosen because of long-standing symptoms, frequent recurrences or poor therapeutic effects. Using Glisson's loop for traction applied to the cervical spine the authors obtained good results, i.e. shortening of the treatment time, and prolongation of improvement. The most frequent complication of this method was vertigo.", "PMID": 980213}
{"id": "pubmed23n0032_12364", "title": "[Spa treatment of shoulder pains at Swierad\u00f3w].", "content": "The authors present the results of spa treatment of painful shoulder in 50 patients. The mean age of patients was 47 years. Radon baths and peat poultices were given to all, most patients had also massage and therapeutic exercises. In 38 cases improvement was obtained, in 6 cases pains decreased, in another 6 cases no improvement was achieved. The authors recommend possibly early balneological treatment in this syndrome.", "contents": "[Spa treatment of shoulder pains at Swierad\u00f3w]. The authors present the results of spa treatment of painful shoulder in 50 patients. The mean age of patients was 47 years. Radon baths and peat poultices were given to all, most patients had also massage and therapeutic exercises. In 38 cases improvement was obtained, in 6 cases pains decreased, in another 6 cases no improvement was achieved. The authors recommend possibly early balneological treatment in this syndrome.", "PMID": 980215}
{"id": "pubmed23n0032_12365", "title": "[Traction and kinesitherapy in the management of painful syndromes of the cervical spine].", "content": "Painful cervical spine syndromes caused by discopathy become ever more frequent. Conservative treatment of cervical discopathy includes mainly traction, kinesitherapy, thermotherapy, massage and electrotherapy. Traction treatment and kinesitherapy are particularly important. The author carried out investigations in 90 cases of cervical discopathy divided into 3 groups of 30 cases in each. In each group cervical traction was applied using Glisson's loop at different traction force and duration of traction. It was observed that three factors were of decisive importance for the end-result of traction: a) a proper position of the patient during the procedure and a proper direction of traction force, b) a proper duration of traction, c) a properly selected weight. Electromyography demonstrated bioelectric activity in the trapezius muscle and deltoid muscle on the affected side before treatment. After application of traction the bioelectric activity of these muscles was significantly reduced at rest. It is concluded that cervical traction and kinesitherapy are effective methods in treatment of cerival spinal painful syndromes.", "contents": "[Traction and kinesitherapy in the management of painful syndromes of the cervical spine]. Painful cervical spine syndromes caused by discopathy become ever more frequent. Conservative treatment of cervical discopathy includes mainly traction, kinesitherapy, thermotherapy, massage and electrotherapy. Traction treatment and kinesitherapy are particularly important. The author carried out investigations in 90 cases of cervical discopathy divided into 3 groups of 30 cases in each. In each group cervical traction was applied using Glisson's loop at different traction force and duration of traction. It was observed that three factors were of decisive importance for the end-result of traction: a) a proper position of the patient during the procedure and a proper direction of traction force, b) a proper duration of traction, c) a properly selected weight. Electromyography demonstrated bioelectric activity in the trapezius muscle and deltoid muscle on the affected side before treatment. After application of traction the bioelectric activity of these muscles was significantly reduced at rest. It is concluded that cervical traction and kinesitherapy are effective methods in treatment of cerival spinal painful syndromes.", "PMID": 980214}
{"id": "pubmed23n0032_12366", "title": "[Spa treatment of sciatic pains at Swierad\u00f3w].", "content": "The authors report therapeutic results obtained in 190 patients with sciatic pains (110 men and 80 women). In 115 cases discopathy was the cause of sciatica, in 75 degenerative vertebral disease was present. The duration of the disease ranged from 6 months to 36 years. Four sets of procedures were used depending on the manifestations of the disease. In evaluation of therapeutic effects the mobility range of the spine, the tone of paravertebral muscles and disappearance of pain were taken into account. Complete improvement was obtained in 76% of cases on the average. It was observed that radon baths had mainly an analgesic effect, peat or paraffin poultices as well as diadynamics were particularly useful in cases with increased tonus of paravertebral muscles. Massage was contraindicated in these cases. Therapeutic exercises were useful in this disease, increasing significantly the mobility range of the spine.", "contents": "[Spa treatment of sciatic pains at Swierad\u00f3w]. The authors report therapeutic results obtained in 190 patients with sciatic pains (110 men and 80 women). In 115 cases discopathy was the cause of sciatica, in 75 degenerative vertebral disease was present. The duration of the disease ranged from 6 months to 36 years. Four sets of procedures were used depending on the manifestations of the disease. In evaluation of therapeutic effects the mobility range of the spine, the tone of paravertebral muscles and disappearance of pain were taken into account. Complete improvement was obtained in 76% of cases on the average. It was observed that radon baths had mainly an analgesic effect, peat or paraffin poultices as well as diadynamics were particularly useful in cases with increased tonus of paravertebral muscles. Massage was contraindicated in these cases. Therapeutic exercises were useful in this disease, increasing significantly the mobility range of the spine.", "PMID": 980216}
{"id": "pubmed23n0032_12367", "title": "Human spinal arachnoid villi and granulations.", "content": "Human spinal arachnoid villi and granulations were studied after distension by a subarachnoid perfusion of Berlin blue and trypan blue. These proliferations were distributed on almost every nerve root in the thoracic and lumbar region. Human spinal arachnoid villi and granulations were divided into those located entirely internal to the dura, those that extended into the dura, and those that penetrated the dura completely. Venous sinuses were closely related to most arachnoid proliferations.", "contents": "Human spinal arachnoid villi and granulations. Human spinal arachnoid villi and granulations were studied after distension by a subarachnoid perfusion of Berlin blue and trypan blue. These proliferations were distributed on almost every nerve root in the thoracic and lumbar region. Human spinal arachnoid villi and granulations were divided into those located entirely internal to the dura, those that extended into the dura, and those that penetrated the dura completely. Venous sinuses were closely related to most arachnoid proliferations.", "PMID": 980235}
{"id": "pubmed23n0032_12368", "title": "Embolization of cerebral angiomas by catheterization of cortical arteries.", "content": "A method of combined direct embolization of submillimetric cortical arteries, after preoperative arteriography, is described and 3 cases of cerebral angiomas presented with striking symptomatic improvement after embolization. Postoperative angiography in one case showed considerable diminution in the size of the angioma.", "contents": "Embolization of cerebral angiomas by catheterization of cortical arteries. A method of combined direct embolization of submillimetric cortical arteries, after preoperative arteriography, is described and 3 cases of cerebral angiomas presented with striking symptomatic improvement after embolization. Postoperative angiography in one case showed considerable diminution in the size of the angioma.", "PMID": 980236}
{"id": "pubmed23n0032_12369", "title": "Radioisotope choroid plexography. A preliminary report.", "content": "Radioisotope plexography seems as easy and innocuous as routine brain scanning. An introductory method is given which could be improved. The choroid plexuses of the four ventricles were well visualized in 15 patients. Consideration is given to the possible labeling process. Besides its morphological value, the test could give interesting clues in the dynamic study of hydrocephalus.", "contents": "Radioisotope choroid plexography. A preliminary report. Radioisotope plexography seems as easy and innocuous as routine brain scanning. An introductory method is given which could be improved. The choroid plexuses of the four ventricles were well visualized in 15 patients. Consideration is given to the possible labeling process. Besides its morphological value, the test could give interesting clues in the dynamic study of hydrocephalus.", "PMID": 980237}
{"id": "pubmed23n0032_12370", "title": "Neurological manifestations of rhabdomyosarcomas in the head and neck in childhood. Neuroradiological study.", "content": "Two cases of rhabdomyosarcoma in the head and neck with neurological manifestations are presented. Brain scan demonstrated an apparent area of increased uptake of 99mTc in the tumor. Cerebral angiography revealed a sharply demarcated but rather faint tumor stain. The contributing vessels were hypertrophied branches of the external carotid artery, a dural branch of the internal carotid artery, and branches of the vetebrobasilar systes emphasized because the recent combination of chemotherapy in conjuction with surgery and irradiation seems to be encouraging.", "contents": "Neurological manifestations of rhabdomyosarcomas in the head and neck in childhood. Neuroradiological study. Two cases of rhabdomyosarcoma in the head and neck with neurological manifestations are presented. Brain scan demonstrated an apparent area of increased uptake of 99mTc in the tumor. Cerebral angiography revealed a sharply demarcated but rather faint tumor stain. The contributing vessels were hypertrophied branches of the external carotid artery, a dural branch of the internal carotid artery, and branches of the vetebrobasilar systes emphasized because the recent combination of chemotherapy in conjuction with surgery and irradiation seems to be encouraging.", "PMID": 980238}
{"id": "pubmed23n0032_12371", "title": "Application of xeroradiography for pneumencephalography.", "content": "The feasibility of xerotomography with pneumencephalography is demonstrated. One of the advantages of a xerotomogram is that it reduces the lack of sharpness caused by tomography. The wide recording latitude of a xerotomogram makes a more detailed evaluation of the bone structure and the outlines of the soft tissue possible when these are contrasted be means of air. The characteristic edge effect of a xerogram also facilitates better detection of the small structures which are less well discerible on a film tomogram.", "contents": "Application of xeroradiography for pneumencephalography. The feasibility of xerotomography with pneumencephalography is demonstrated. One of the advantages of a xerotomogram is that it reduces the lack of sharpness caused by tomography. The wide recording latitude of a xerotomogram makes a more detailed evaluation of the bone structure and the outlines of the soft tissue possible when these are contrasted be means of air. The characteristic edge effect of a xerogram also facilitates better detection of the small structures which are less well discerible on a film tomogram.", "PMID": 980239}
{"id": "pubmed23n0032_12372", "title": "The third ventricle. Normal anatomy and changes in some pathological conditions.", "content": "An anatomical study of the normal anterior inferior part and the floor of the third ventricle has been made and its radiological appearance shown. Specimens and casts were analyzed and correlated with ventriculograms using watersoluble contrast media. The detailed appearance of the optic recess and sulcus of Monro, which has not been well described in the neuroradiological literature, is illustrated and detailed. The changes in the optic recess in hydrocephalus are discussed. Deformities of the different segments of the floor as a result of tumors of the posterior fossa and hydrocephalus are shown.", "contents": "The third ventricle. Normal anatomy and changes in some pathological conditions. An anatomical study of the normal anterior inferior part and the floor of the third ventricle has been made and its radiological appearance shown. Specimens and casts were analyzed and correlated with ventriculograms using watersoluble contrast media. The detailed appearance of the optic recess and sulcus of Monro, which has not been well described in the neuroradiological literature, is illustrated and detailed. The changes in the optic recess in hydrocephalus are discussed. Deformities of the different segments of the floor as a result of tumors of the posterior fossa and hydrocephalus are shown.", "PMID": 980240}
{"id": "pubmed23n0032_12373", "title": "Aqueductal stenosis and hydrocephalus: rare sequelae of mumps virus infections.", "content": "A case of a rare acquired form of aqueductal stenosis and hydrocephalus developing as a late sequela of mumps virus infection of the central nervous system is presented. The experimental studies of mumps induced aqueductal stenosis in hamsters and mice and the four previously reported cases in the literature are reviewed.", "contents": "Aqueductal stenosis and hydrocephalus: rare sequelae of mumps virus infections. A case of a rare acquired form of aqueductal stenosis and hydrocephalus developing as a late sequela of mumps virus infection of the central nervous system is presented. The experimental studies of mumps induced aqueductal stenosis in hamsters and mice and the four previously reported cases in the literature are reviewed.", "PMID": 980242}
{"id": "pubmed23n0032_12374", "title": "Spinal cord angiomas diagnosed by gas myelography.", "content": "Three cases with spinal cord arteriovenous malformations are reported with characteristic changes evident on gas myelography. The malformations were confirmed by selective spinal angiography in all cases and surgically removed in 2 of the cases.", "contents": "Spinal cord angiomas diagnosed by gas myelography. Three cases with spinal cord arteriovenous malformations are reported with characteristic changes evident on gas myelography. The malformations were confirmed by selective spinal angiography in all cases and surgically removed in 2 of the cases.", "PMID": 980243}
{"id": "pubmed23n0032_12375", "title": "Unilateral endocrine exophthalmos. Diagnostic problems in association with computed tomography.", "content": "Swollen orbital extraocular muscles may mimic an orbital apex tumor on computed tomography. In five of our patients, in spite of highly suggestive findings on CT scanning, indicating the presence of an orbital apex lesion, the correct diagnosis proved to be endocrine exophthalmos. The value of complementary CT projections and of orbital ultrasonography to assist in the differential diagnosis is discussed, and the additional contribution of orbital phlebography for the confirmation of lesions in the orbital apex is emphasized.", "contents": "Unilateral endocrine exophthalmos. Diagnostic problems in association with computed tomography. Swollen orbital extraocular muscles may mimic an orbital apex tumor on computed tomography. In five of our patients, in spite of highly suggestive findings on CT scanning, indicating the presence of an orbital apex lesion, the correct diagnosis proved to be endocrine exophthalmos. The value of complementary CT projections and of orbital ultrasonography to assist in the differential diagnosis is discussed, and the additional contribution of orbital phlebography for the confirmation of lesions in the orbital apex is emphasized.", "PMID": 980244}
{"id": "pubmed23n0032_12376", "title": "Widening of the cervical intervertebral foramen.", "content": "Case reports of seven patients in whom unusual abnormalities caused widening of the cervical intervertebral foramen are presented. Plain roentgenographic and tomographic changes may be quite distinctive, especially in a patient with an absent pedicle or a tortuous vertebral artery. However, angiography has proved to be a useful adjunct to determine the etiology in many of these lesions.", "contents": "Widening of the cervical intervertebral foramen. Case reports of seven patients in whom unusual abnormalities caused widening of the cervical intervertebral foramen are presented. Plain roentgenographic and tomographic changes may be quite distinctive, especially in a patient with an absent pedicle or a tortuous vertebral artery. However, angiography has proved to be a useful adjunct to determine the etiology in many of these lesions.", "PMID": 980245}
{"id": "pubmed23n0032_12377", "title": "Computer tomography of the cerebrum in multiple sclerosis.", "content": "One hundred and ten patients with multiple sclerosis (MS) were examinated with computer tomography (CT) of the brain with the 160 X 160 matrix EMI scanner. Eighty-two areas of low X-ray attenuation around the ventricular system were found in 40 cases (36%), particularly adjacent to the anterior and posterior horns and trigones. These brain volumes with low attenuation are considered to represent the well known large periventricular plaques of MS. The mean plaque volume was 1.26 cm3 and mean attenuation value 9.5 EMI units, which was more than 5 units lower than that of the surrounding brain tissue. Contrast enhancement provided no further information. Periventricular plaques were concomitant to ventricular enlargement and cortical atrophy. By comparison with normal values 49 cases (45%) with atrophy and no plaques were found, while only 20 MS patients (18%) had normal EMI scans. There was no difference between duration of disease and age at onset in the three different groups of MS cases.", "contents": "Computer tomography of the cerebrum in multiple sclerosis. One hundred and ten patients with multiple sclerosis (MS) were examinated with computer tomography (CT) of the brain with the 160 X 160 matrix EMI scanner. Eighty-two areas of low X-ray attenuation around the ventricular system were found in 40 cases (36%), particularly adjacent to the anterior and posterior horns and trigones. These brain volumes with low attenuation are considered to represent the well known large periventricular plaques of MS. The mean plaque volume was 1.26 cm3 and mean attenuation value 9.5 EMI units, which was more than 5 units lower than that of the surrounding brain tissue. Contrast enhancement provided no further information. Periventricular plaques were concomitant to ventricular enlargement and cortical atrophy. By comparison with normal values 49 cases (45%) with atrophy and no plaques were found, while only 20 MS patients (18%) had normal EMI scans. There was no difference between duration of disease and age at onset in the three different groups of MS cases.", "PMID": 980246}
{"id": "pubmed23n0032_12378", "title": "The DES story.", "content": "During the last 6 years, there has been a marked increase in the incidence of vaginal and cervical clear cell adenocarcinomas occurring in young females. A relationship between maternal ingestion of synthetic estrogens occurs in about 75% of these cases. DES was used in a variety of pregnancy complications between 1940 and 1970 with the peak years being 1945 through 1960. There is no explanation for the increased number of cases occurring in patients whose mothers did not ingest synthetic estrogens. The number of women who took these drugs during pregnancy and their at-risk daughters is unknown. Vaginal adenosis is an associated finding in about 90% of the vaginal and 30% of the cervical adenocarcinomas. The reported incidence of adenosis in DES-exposed offspring varies from 30 to 90%. Gross cervicovaginal abnormalities occur in about 20% of the exposed patients. The relationship between adenosis and clear cell adenocarcinoma is not clear; however, many investigators feel adenosis is a precursor. Both adenosis and the clear cell tumors are of M\u00fcllerian origin, and it appears that any dose of synthetic estrogens ingested prior to the 18th week of pregnancy alters the normal embryogenesis of the vagina.", "contents": "The DES story. During the last 6 years, there has been a marked increase in the incidence of vaginal and cervical clear cell adenocarcinomas occurring in young females. A relationship between maternal ingestion of synthetic estrogens occurs in about 75% of these cases. DES was used in a variety of pregnancy complications between 1940 and 1970 with the peak years being 1945 through 1960. There is no explanation for the increased number of cases occurring in patients whose mothers did not ingest synthetic estrogens. The number of women who took these drugs during pregnancy and their at-risk daughters is unknown. Vaginal adenosis is an associated finding in about 90% of the vaginal and 30% of the cervical adenocarcinomas. The reported incidence of adenosis in DES-exposed offspring varies from 30 to 90%. Gross cervicovaginal abnormalities occur in about 20% of the exposed patients. The relationship between adenosis and clear cell adenocarcinoma is not clear; however, many investigators feel adenosis is a precursor. Both adenosis and the clear cell tumors are of M\u00fcllerian origin, and it appears that any dose of synthetic estrogens ingested prior to the 18th week of pregnancy alters the normal embryogenesis of the vagina.", "PMID": 980278}
{"id": "pubmed23n0032_12379", "title": "Cytology of 575 young women with prenatal exposure to diethylstilbestrol.", "content": "The vaginal and cervical cellular changes encountered in 575 postpubertal females exposed prenatally to diethylstilbestrol (DES) were compared with those of an unexposed population with particular reference to the role of cytology in the detection of vaginal adenosis and cervical ectropion (erosion). Several methods of obtaining specimens were utilized, the most effcacious of which was scraping of the vagina, especially the fornices, and the portio vaginalis of the cervix. With this technic, columnar cells of the mucinous type and metaplastic squamous cells were observed in 34% of the vaginal scrapes and 54% of the scrapes of the cervical portio. A higher incidence was apparent among those patients in whom iodine staining of the vaginal mucosa was abnormal or vaginal adenosis was proven by biopsy. Moderate to severe dysplasia of the squamous cells or atypical glandular cells were found in 1% of the exposed subjects. This study indicates that the presence of mucinous columnar or metaplastic squamous cells in vaginal scrapes is suggestive of vaginal adenosis but that vaginal cytology cannot be considered a uniformly reliable screening technic for detecting the presence of this disorder.", "contents": "Cytology of 575 young women with prenatal exposure to diethylstilbestrol. The vaginal and cervical cellular changes encountered in 575 postpubertal females exposed prenatally to diethylstilbestrol (DES) were compared with those of an unexposed population with particular reference to the role of cytology in the detection of vaginal adenosis and cervical ectropion (erosion). Several methods of obtaining specimens were utilized, the most effcacious of which was scraping of the vagina, especially the fornices, and the portio vaginalis of the cervix. With this technic, columnar cells of the mucinous type and metaplastic squamous cells were observed in 34% of the vaginal scrapes and 54% of the scrapes of the cervical portio. A higher incidence was apparent among those patients in whom iodine staining of the vaginal mucosa was abnormal or vaginal adenosis was proven by biopsy. Moderate to severe dysplasia of the squamous cells or atypical glandular cells were found in 1% of the exposed subjects. This study indicates that the presence of mucinous columnar or metaplastic squamous cells in vaginal scrapes is suggestive of vaginal adenosis but that vaginal cytology cannot be considered a uniformly reliable screening technic for detecting the presence of this disorder.", "PMID": 980279}
{"id": "pubmed23n0032_12380", "title": "The use of prophylactic partial exchange tranfusion in pregnancies associated with sickle cell hemoglobinopathies.", "content": "Sickle cell anemia is associated with an alarming attrition rate during pregnancy. The maternal morbidity rate, perinatal wastage rate, and the incidence of severe morbidity in both mother and child are elevated above acceptable limits. In most cases, these statistics have been compiled using conservative therapeutic modalities. In contrast, this report utilizes prophylactic partial exchange transfusion therapy in patients with severe sickle cell hemoglobinopathies. The protocol involves the introduction of 750-1000 cc of buffy coat, poor washed red cells exchanged with 1000-1500 cc whole blood during phlebotomy at 28 weeks' gestation and again prior to term. Thirty-six consecutive pregnant patients with sickle cell anemia have been managed in this fashion. The one maternal mortality occurred in a patient who did not complete the protocol. Major maternal morbidity and perinatal wastage rates were significantly decreased. Two cases of serum hepatitis occurred. It appears from these data that the use of prophylactic partial exchange transfusion in pregnant patients with severe sickle cell hemoglobinopathies can be of benefit. Further trials of this method seem justified by these results to assess completely the benefit-risk ratio of this procedure.", "contents": "The use of prophylactic partial exchange tranfusion in pregnancies associated with sickle cell hemoglobinopathies. Sickle cell anemia is associated with an alarming attrition rate during pregnancy. The maternal morbidity rate, perinatal wastage rate, and the incidence of severe morbidity in both mother and child are elevated above acceptable limits. In most cases, these statistics have been compiled using conservative therapeutic modalities. In contrast, this report utilizes prophylactic partial exchange transfusion therapy in patients with severe sickle cell hemoglobinopathies. The protocol involves the introduction of 750-1000 cc of buffy coat, poor washed red cells exchanged with 1000-1500 cc whole blood during phlebotomy at 28 weeks' gestation and again prior to term. Thirty-six consecutive pregnant patients with sickle cell anemia have been managed in this fashion. The one maternal mortality occurred in a patient who did not complete the protocol. Major maternal morbidity and perinatal wastage rates were significantly decreased. Two cases of serum hepatitis occurred. It appears from these data that the use of prophylactic partial exchange transfusion in pregnant patients with severe sickle cell hemoglobinopathies can be of benefit. Further trials of this method seem justified by these results to assess completely the benefit-risk ratio of this procedure.", "PMID": 980280}
{"id": "pubmed23n0032_12381", "title": "Vasopressin response and terbutaline inhibition of the uterus.", "content": "A study of the effects of 8-lysine vasopressin (LVP) and its long-acting analogue N-a-triglycyl-8-lysine vasopressin (TGVP) on the myometrium and vasculature of the normal human nonpregnant uterus was undertaken. The results suggest that the vasopressins decrease local endometrial blood flow both directly by effects on the uterine vascular bed, and indirectly by increasing myometrial activity. The effects in vivo can be effectively counteracted by beta2 receptor stimulation.", "contents": "Vasopressin response and terbutaline inhibition of the uterus. A study of the effects of 8-lysine vasopressin (LVP) and its long-acting analogue N-a-triglycyl-8-lysine vasopressin (TGVP) on the myometrium and vasculature of the normal human nonpregnant uterus was undertaken. The results suggest that the vasopressins decrease local endometrial blood flow both directly by effects on the uterine vascular bed, and indirectly by increasing myometrial activity. The effects in vivo can be effectively counteracted by beta2 receptor stimulation.", "PMID": 980281}
{"id": "pubmed23n0032_12382", "title": "Enterovaginal and enterocutaneous fistulae in women with gynecologic malignancies.", "content": "From 1957 to 1975, 43 women with gynecologic malignancies had associated enterocutaneous or enterovaginal fistula. Thirty-four of these women underwent surgical correction of the fistula. The etiology, diagnostic evaluation, preoperative complications, and surgical correction of such fistulae are discussed. In addition, a staged planned management of these fistulae is outlined.", "contents": "Enterovaginal and enterocutaneous fistulae in women with gynecologic malignancies. From 1957 to 1975, 43 women with gynecologic malignancies had associated enterocutaneous or enterovaginal fistula. Thirty-four of these women underwent surgical correction of the fistula. The etiology, diagnostic evaluation, preoperative complications, and surgical correction of such fistulae are discussed. In addition, a staged planned management of these fistulae is outlined.", "PMID": 980282}
{"id": "pubmed23n0032_12383", "title": "Vulvar histology after neutral red photoinactivation of herpes simplex virus.", "content": "The use of photodynamic dye and light inactivation for the treatment of genital herpes simplex virus infections has been associated with the risk of potential oncogenesis. Sixteen patients treated with neutral red and fluorescent light for documented herpetic infections were studied at intervals ranging from 9 to 52 months following treatment. Four patients treated with other modalities were included in the study. Biopsies of the treated areas were obtained, and 3925 tissue sections were examined. Mild atypical epithelial changes were focally present in most specimens regardless of therapy. Histologically identifiable premalignant change could not be demonstrated.", "contents": "Vulvar histology after neutral red photoinactivation of herpes simplex virus. The use of photodynamic dye and light inactivation for the treatment of genital herpes simplex virus infections has been associated with the risk of potential oncogenesis. Sixteen patients treated with neutral red and fluorescent light for documented herpetic infections were studied at intervals ranging from 9 to 52 months following treatment. Four patients treated with other modalities were included in the study. Biopsies of the treated areas were obtained, and 3925 tissue sections were examined. Mild atypical epithelial changes were focally present in most specimens regardless of therapy. Histologically identifiable premalignant change could not be demonstrated.", "PMID": 980283}
{"id": "pubmed23n0032_12384", "title": "Microinvasive carcinoma of the cervix.", "content": "The clinical and pathologic features of 51 cases of microinvasive carcinoma of the cervix treated by radical hysterectomy and pelvic lymphadenectomy are presented. Microinvasion was defined as stromal penetration by carcinoma not exceeding a depth of 5 mm from the surface at the point of origin. Patients with confluent patterns and lymphatic invasion were not excluded. Lymphatic invasion was demonstrated in 24% of the patients, but none of the resected lymph nodes from the entire series contained metastatic tumor. Residual invasive disease was present in 9 of 47 patients who underwent conization including 1 in which the residual tumor invaded to 8 mm, although the depth of invasion in the cone biopsy was only 2.5 mm. Factors related to the presence of residual invasive disease included the pattern and extent of invasion and involvement of the cone margin. There were no surgery-related deaths or fistulae in this series; the actuarial survival rate at 5 years was 100%. Simple hysterectomy seems justified if the cone margin is free of tumor since none of these patients has residual disease. In contrast, radical hysterectomy may be indicated if the cone margins are involved in view of high frequency of residual tumor (39%) and the possibility of invasion in the cervix exceeding 5 mm.", "contents": "Microinvasive carcinoma of the cervix. The clinical and pathologic features of 51 cases of microinvasive carcinoma of the cervix treated by radical hysterectomy and pelvic lymphadenectomy are presented. Microinvasion was defined as stromal penetration by carcinoma not exceeding a depth of 5 mm from the surface at the point of origin. Patients with confluent patterns and lymphatic invasion were not excluded. Lymphatic invasion was demonstrated in 24% of the patients, but none of the resected lymph nodes from the entire series contained metastatic tumor. Residual invasive disease was present in 9 of 47 patients who underwent conization including 1 in which the residual tumor invaded to 8 mm, although the depth of invasion in the cone biopsy was only 2.5 mm. Factors related to the presence of residual invasive disease included the pattern and extent of invasion and involvement of the cone margin. There were no surgery-related deaths or fistulae in this series; the actuarial survival rate at 5 years was 100%. Simple hysterectomy seems justified if the cone margin is free of tumor since none of these patients has residual disease. In contrast, radical hysterectomy may be indicated if the cone margins are involved in view of high frequency of residual tumor (39%) and the possibility of invasion in the cervix exceeding 5 mm.", "PMID": 980284}
{"id": "pubmed23n0032_12385", "title": "Cell-mediated immunity and blocking factor in ovarian carcinoma.", "content": "Lymphocyte-mediated cytotoxicity (cell-mediated immunity) to ovarian carcinoma cells and serum blocking factor were measured in 37 patients. Short-term cultures of tumor cells and a low ratio of effector to target cells were used throughout the study, minimizing nonspecific cytotoxicity. Sixteen patients were followed for long periods of time, and correlation with the course of the disease and with therapy could be obtained. Although the level of cell-mediated immunity did not always correspond to the clinical status of the patient, the presence of blocking factor was associated with clinical relapse in 14 of 16 patients. Chemotherapy with single alkylating agents or combinations of drugs caused no significant or permanent depression of cell-mediated immunity as measured in this way. In addition, blocking factor disappeared in 2 patients during remission. It appears that the chemotherapy for ovarian carcinoma may not be significantly immunosuppressive against established levels of cell-mediated immunity and may in certain instances have effects potentially beneficial to the host as evaluated by lymphocyte-mediated cytotoxicity and blocking factor studies.", "contents": "Cell-mediated immunity and blocking factor in ovarian carcinoma. Lymphocyte-mediated cytotoxicity (cell-mediated immunity) to ovarian carcinoma cells and serum blocking factor were measured in 37 patients. Short-term cultures of tumor cells and a low ratio of effector to target cells were used throughout the study, minimizing nonspecific cytotoxicity. Sixteen patients were followed for long periods of time, and correlation with the course of the disease and with therapy could be obtained. Although the level of cell-mediated immunity did not always correspond to the clinical status of the patient, the presence of blocking factor was associated with clinical relapse in 14 of 16 patients. Chemotherapy with single alkylating agents or combinations of drugs caused no significant or permanent depression of cell-mediated immunity as measured in this way. In addition, blocking factor disappeared in 2 patients during remission. It appears that the chemotherapy for ovarian carcinoma may not be significantly immunosuppressive against established levels of cell-mediated immunity and may in certain instances have effects potentially beneficial to the host as evaluated by lymphocyte-mediated cytotoxicity and blocking factor studies.", "PMID": 980285}
{"id": "pubmed23n0032_12386", "title": "Hormonal findings associated with aluteal cycles.", "content": "Menstrual bleeding with no detectable luteal function was observed cyclicly in 3 infertile patients. Anovulation was suspected and documented by basal body temperature charts and repeated endometrial biopsies prior to expected menses. A sporadic anovulatory cycle in a normally ovulating volunteer was studied and the hormonal parameters included for comparison. To elucidate the significance and the possible cause of this defect, five aluteal cycles were evaluated with serum follicle stimulating hormone (FSH), luteinizing hormone (LH), total estrogen, progestin, and prolactin determinations. No similar patterns of FSH, LH, or estrogen values were observed. No abnormality of prolactin levels was noted. The lack of progestin rise and proliferative endometrial biopsies during the second half of these cycles were the only consistent findings. When clomiphene citrate therapy was instituted, a luteal phase was promptly established.", "contents": "Hormonal findings associated with aluteal cycles. Menstrual bleeding with no detectable luteal function was observed cyclicly in 3 infertile patients. Anovulation was suspected and documented by basal body temperature charts and repeated endometrial biopsies prior to expected menses. A sporadic anovulatory cycle in a normally ovulating volunteer was studied and the hormonal parameters included for comparison. To elucidate the significance and the possible cause of this defect, five aluteal cycles were evaluated with serum follicle stimulating hormone (FSH), luteinizing hormone (LH), total estrogen, progestin, and prolactin determinations. No similar patterns of FSH, LH, or estrogen values were observed. No abnormality of prolactin levels was noted. The lack of progestin rise and proliferative endometrial biopsies during the second half of these cycles were the only consistent findings. When clomiphene citrate therapy was instituted, a luteal phase was promptly established.", "PMID": 980286}
{"id": "pubmed23n0032_12387", "title": "Corpus luteum function during pregnancies of previously infertile women.", "content": "Seventy-nine previously infertile women were studied during the first 9 weeks of their pregnancies. Pregnancy had occurred either without any specific therapy or following treatment with clomiphene, prednisone, or human chorionic gonadotropin, singly or in various combinations. Serum levels of progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured at 2-week intervals. A profile of early pregnancy in terms of these hormones is presented and discussed with regard to its significance for corpus luteum function. Aspects of the hormonal profile predictive of the pregnancy outcome are noted. Ovarian over-stimulation was directly related to the dosage of clomiphene, and the increased levels of 17 alpha-hydroxyprogesterone thus produced persisted into the seventh week of pregnancy. This hormonal excess probably derives from multiple corpora lutea which may also account for the increased incidence of multiple pregnancy associated with clomiphene therapy.", "contents": "Corpus luteum function during pregnancies of previously infertile women. Seventy-nine previously infertile women were studied during the first 9 weeks of their pregnancies. Pregnancy had occurred either without any specific therapy or following treatment with clomiphene, prednisone, or human chorionic gonadotropin, singly or in various combinations. Serum levels of progesterone, 17 alpha-hydroxyprogesterone, and estradiol were measured at 2-week intervals. A profile of early pregnancy in terms of these hormones is presented and discussed with regard to its significance for corpus luteum function. Aspects of the hormonal profile predictive of the pregnancy outcome are noted. Ovarian over-stimulation was directly related to the dosage of clomiphene, and the increased levels of 17 alpha-hydroxyprogesterone thus produced persisted into the seventh week of pregnancy. This hormonal excess probably derives from multiple corpora lutea which may also account for the increased incidence of multiple pregnancy associated with clomiphene therapy.", "PMID": 980287}
{"id": "pubmed23n0032_12388", "title": "Correlation between amniotic fluid optical density and L/S ratio.", "content": "A test that circumvents the complex methodology needed for the determination of L/S ratios has been developed. It has been observed that the optical density, measured at 400 nm, of supernatants collected from fresh amniotic fluids centrifuged at 2000 g for 10 minutes correlates with L/S ratios.", "contents": "Correlation between amniotic fluid optical density and L/S ratio. A test that circumvents the complex methodology needed for the determination of L/S ratios has been developed. It has been observed that the optical density, measured at 400 nm, of supernatants collected from fresh amniotic fluids centrifuged at 2000 g for 10 minutes correlates with L/S ratios.", "PMID": 980289}
{"id": "pubmed23n0032_12389", "title": "Giant sertoli cell adenoma in testicular feminization syndrome.", "content": "An extremely large Sertoli cell adenoma discovered incidentally in an elderly phenotypically femal patient with testicular feminization syndrome is reported. The patient is alive 3 years following removal of the tumor.", "contents": "Giant sertoli cell adenoma in testicular feminization syndrome. An extremely large Sertoli cell adenoma discovered incidentally in an elderly phenotypically femal patient with testicular feminization syndrome is reported. The patient is alive 3 years following removal of the tumor.", "PMID": 980290}
{"id": "pubmed23n0032_12390", "title": "Laceration of umbilical cord vessels secondary to amniocentesis.", "content": "Amniocentesis has been established as a valuable diagnostic tool in the management of high-risk pregnancies. Although infrequent, various fetal complications have been reported. A serious and potentially fatal complication is laceration of the umbilical cord vessels. Three cases are presented to illustrate this complication and a scheme is proposed for management when bloody fluid is obtained at the time of amniocentesis. If the bleeding is of fetal origin, and the fetus is at or near term, immediate delivery should be considered. Expectant management should be considered only in the premature fetus when continuous fetal heart rate monitoring is possible and no bradycardia is encountered. Induction and vaginal delivery should be attempted only in the absence of a bradycardia and if the patient is able to be continuously monitored. Postamniocentesis fetal bradycardia should prompt immediate delivery via cesarean section regardless of gestational age. Any newborn whose uterine existence has been complicated by a traumatic or bloody tap should have an immediate hematocrit determination at birth.", "contents": "Laceration of umbilical cord vessels secondary to amniocentesis. Amniocentesis has been established as a valuable diagnostic tool in the management of high-risk pregnancies. Although infrequent, various fetal complications have been reported. A serious and potentially fatal complication is laceration of the umbilical cord vessels. Three cases are presented to illustrate this complication and a scheme is proposed for management when bloody fluid is obtained at the time of amniocentesis. If the bleeding is of fetal origin, and the fetus is at or near term, immediate delivery should be considered. Expectant management should be considered only in the premature fetus when continuous fetal heart rate monitoring is possible and no bradycardia is encountered. Induction and vaginal delivery should be attempted only in the absence of a bradycardia and if the patient is able to be continuously monitored. Postamniocentesis fetal bradycardia should prompt immediate delivery via cesarean section regardless of gestational age. Any newborn whose uterine existence has been complicated by a traumatic or bloody tap should have an immediate hematocrit determination at birth.", "PMID": 980291}
{"id": "pubmed23n0032_12391", "title": "Electron microscopic observations on rat epidermis during experimental carcinogenesis.", "content": "Early ultrastructural changes and their progressive evolution were studied in rat epidermis following topical application of carcinogen (3-methylcholanthrene) and were compared to ultrastructural features of fully developed tumors (sebaceous epithelioma and basal cell epithelioma) in animals of the same group. Similar cytologic alterations were found, such as a marked increase in free ribosomes and polysomes, extensive endoplasmic reticulum, considerable increase of mitochondria, and dilated intercellular spaces, occupied by cytoplasmic projections. Predominance of dark cells, considered by us to be only phenotypically different from normal (light) cells, was also observed. A two-stage or multiple-stage of neoplastic evolution was suggested.", "contents": "Electron microscopic observations on rat epidermis during experimental carcinogenesis. Early ultrastructural changes and their progressive evolution were studied in rat epidermis following topical application of carcinogen (3-methylcholanthrene) and were compared to ultrastructural features of fully developed tumors (sebaceous epithelioma and basal cell epithelioma) in animals of the same group. Similar cytologic alterations were found, such as a marked increase in free ribosomes and polysomes, extensive endoplasmic reticulum, considerable increase of mitochondria, and dilated intercellular spaces, occupied by cytoplasmic projections. Predominance of dark cells, considered by us to be only phenotypically different from normal (light) cells, was also observed. A two-stage or multiple-stage of neoplastic evolution was suggested.", "PMID": 980364}
{"id": "pubmed23n0032_12392", "title": "Carcinogenicity of Benzo(a)pyrene and dusts in the hamster lung (instilled intratracheally with titanium oxide, aluminum oxide, carbon and ferric oxide).", "content": "The possible carcinogenic or fibrogenic effects of intratracheal instillation of a polycyclic hydrocarbon, benzo(a) pyrene (B(a)P), alone or in combination with several dusts - titanium dioxide (TiO2), aluminum oxide (Al2O3), carbon (C), and ferric oxide (Fe3O3) - were investigated in hamsters. When administered alone, the dusts induced interstitial cell proliferation, bronchial epithelial alterations and a few granulomatous changes in the pulmonary system, but no tumors. B(a)P alone induced only two tracheal papillomas. However, combined treatment with B(a)P and the dusts caused a number of tumors, dependent upon the dust used. B(a)P plus TiO2 in a small particle size (below 0.5mu) induced papillomas, squamous cell carcinomas and a few adenomas and adenocarcinomas of the larynx, trachea and lungs. These were morpholoigcally similar to neoplasms found after B(a)P and Fe2O3 treatment. B(a)P, combined with C or Al2O3, induced mainly laryngeal and tracheal papillomas. B(a)P- and C-treated hamsters also showed a few lung adenocarcinomas.", "contents": "Carcinogenicity of Benzo(a)pyrene and dusts in the hamster lung (instilled intratracheally with titanium oxide, aluminum oxide, carbon and ferric oxide). The possible carcinogenic or fibrogenic effects of intratracheal instillation of a polycyclic hydrocarbon, benzo(a) pyrene (B(a)P), alone or in combination with several dusts - titanium dioxide (TiO2), aluminum oxide (Al2O3), carbon (C), and ferric oxide (Fe3O3) - were investigated in hamsters. When administered alone, the dusts induced interstitial cell proliferation, bronchial epithelial alterations and a few granulomatous changes in the pulmonary system, but no tumors. B(a)P alone induced only two tracheal papillomas. However, combined treatment with B(a)P and the dusts caused a number of tumors, dependent upon the dust used. B(a)P plus TiO2 in a small particle size (below 0.5mu) induced papillomas, squamous cell carcinomas and a few adenomas and adenocarcinomas of the larynx, trachea and lungs. These were morpholoigcally similar to neoplasms found after B(a)P and Fe2O3 treatment. B(a)P, combined with C or Al2O3, induced mainly laryngeal and tracheal papillomas. B(a)P- and C-treated hamsters also showed a few lung adenocarcinomas.", "PMID": 980365}
{"id": "pubmed23n0032_12393", "title": "Methodoloigcal study on examiniation of primary tumors of the respiratory tract \"in toto\" and histologically.", "content": "The results of histological evaluation of DENA-induced tracheobronchial tumors in the Syrian golden hamster are described and compared with those of an in toto method of visualization. In 55 animals, roughly twice as many tumors were detected in the lungs in the in toto preparation as those found by the histological technique [92:42]. The examiniation of the respiratory tract, as a whole, would consequently appear to afford an efficient and economical means of quantifying the effects of, for instance, chemotherapeutic agents in the growth of such tumors, which can subsequently be complemented, especially in the qualitative sense, by histological techniques.", "contents": "Methodoloigcal study on examiniation of primary tumors of the respiratory tract \"in toto\" and histologically. The results of histological evaluation of DENA-induced tracheobronchial tumors in the Syrian golden hamster are described and compared with those of an in toto method of visualization. In 55 animals, roughly twice as many tumors were detected in the lungs in the in toto preparation as those found by the histological technique [92:42]. The examiniation of the respiratory tract, as a whole, would consequently appear to afford an efficient and economical means of quantifying the effects of, for instance, chemotherapeutic agents in the growth of such tumors, which can subsequently be complemented, especially in the qualitative sense, by histological techniques.", "PMID": 980366}
{"id": "pubmed23n0032_12394", "title": "Skeletal metastases in pancreatic carcinoma: study by isotopic bone scanning.", "content": "A review of the literature of 2,155 reported patients with primary carcinoma of the pancreas, revealed 110 cases or 5% to have skeletal metastasis by radiographic or autopsy study. A study conducted over a 2 year period disclosed that 1 case of skeletal metastasis was detected by bone scanning in 16 patients with pancreatic carcinoma. This indicates a minimum skeletal metastasis rate of 6%. We feel these percentages are low and can be further defined by the more routine employment of the bone scan to evaluate patients with carcinoma of the pancreas. The true figure may be much higher, perhaps as high as 20%.", "contents": "Skeletal metastases in pancreatic carcinoma: study by isotopic bone scanning. A review of the literature of 2,155 reported patients with primary carcinoma of the pancreas, revealed 110 cases or 5% to have skeletal metastasis by radiographic or autopsy study. A study conducted over a 2 year period disclosed that 1 case of skeletal metastasis was detected by bone scanning in 16 patients with pancreatic carcinoma. This indicates a minimum skeletal metastasis rate of 6%. We feel these percentages are low and can be further defined by the more routine employment of the bone scan to evaluate patients with carcinoma of the pancreas. The true figure may be much higher, perhaps as high as 20%.", "PMID": 980367}
{"id": "pubmed23n0032_12395", "title": "Chemical carcinogens in the human environment; problems and quantitative aspects.", "content": "A short review on environmental carcinogenesis by chemicals is given. Problems related with the determination of carcinogenicity of chemicals are mentioned. Some critical factors in the monitoring of environmental carcinogens are discussed in relation to a quantification of human exposure to such compounds. An attempt at risk evaluation is made for polycyclic aromatic hydrocarbons, vinyl chloride, N-nitroso compounds and aflatoxins. It is concluded that there is a \"threshold dose\" for individual carcinogens that does not lead to tumor induction within the normal life span. The probability of multifactorial cause of human cancer by environmental carcinogens, however, does not allow the definition of \"safe\" levels of such compounds in view of syncarcinogenic and other enhancing and/or modifying effects.", "contents": "Chemical carcinogens in the human environment; problems and quantitative aspects. A short review on environmental carcinogenesis by chemicals is given. Problems related with the determination of carcinogenicity of chemicals are mentioned. Some critical factors in the monitoring of environmental carcinogens are discussed in relation to a quantification of human exposure to such compounds. An attempt at risk evaluation is made for polycyclic aromatic hydrocarbons, vinyl chloride, N-nitroso compounds and aflatoxins. It is concluded that there is a \"threshold dose\" for individual carcinogens that does not lead to tumor induction within the normal life span. The probability of multifactorial cause of human cancer by environmental carcinogens, however, does not allow the definition of \"safe\" levels of such compounds in view of syncarcinogenic and other enhancing and/or modifying effects.", "PMID": 980368}
{"id": "pubmed23n0032_12396", "title": "Evaluation of environmental carcinogens for cancer in man.", "content": "The problem of the presence of carcinogens in the environment has become of considerable concern to the public, as it has been to the biomedical community for some time. The burden of identifying carcinogenic agents and determining threshold levels, if they exist, falls upon the scientific community. Particular care must be exercised in the design, conduct and analysis of experiments directed toward the identification of carcinogens. The data generated from such tests provides the basis for regulatory decisions by governmental authorities. The advice provided by scientists must be based on sound data. There have been instances, however, and there will be others, in which biological variability and the limits of sensitivity of our tests will give equivocal results. It is the duty of scientists to bring such uncertainties to the attention of the public while working towards the development of more sensitive and precise techniques.", "contents": "Evaluation of environmental carcinogens for cancer in man. The problem of the presence of carcinogens in the environment has become of considerable concern to the public, as it has been to the biomedical community for some time. The burden of identifying carcinogenic agents and determining threshold levels, if they exist, falls upon the scientific community. Particular care must be exercised in the design, conduct and analysis of experiments directed toward the identification of carcinogens. The data generated from such tests provides the basis for regulatory decisions by governmental authorities. The advice provided by scientists must be based on sound data. There have been instances, however, and there will be others, in which biological variability and the limits of sensitivity of our tests will give equivocal results. It is the duty of scientists to bring such uncertainties to the attention of the public while working towards the development of more sensitive and precise techniques.", "PMID": 980369}
{"id": "pubmed23n0032_12397", "title": "Mathematical aspects of dose-response studies in carcinogenesis--the concept of thresholds.", "content": "Many of the previously proposed theories of carcinogenesis which relate the exposure level of a carcinogen to expected tumor response, are summarized and it is pointed out that these theories assume the existence of no threshold level. A modification of the one-hit carcinogenic model which would incorporate a threshold, is proposed along with a generalization to variable thresholds over the population at risk. The statistical problems of discriminating between threshold and non-threshold models from experimental bioassay data are discussed and an example is given.", "contents": "Mathematical aspects of dose-response studies in carcinogenesis--the concept of thresholds. Many of the previously proposed theories of carcinogenesis which relate the exposure level of a carcinogen to expected tumor response, are summarized and it is pointed out that these theories assume the existence of no threshold level. A modification of the one-hit carcinogenic model which would incorporate a threshold, is proposed along with a generalization to variable thresholds over the population at risk. The statistical problems of discriminating between threshold and non-threshold models from experimental bioassay data are discussed and an example is given.", "PMID": 980370}
{"id": "pubmed23n0032_12398", "title": "Some examples of dose-response studies in chemical carcinogenesis.", "content": "Three studies of DRUCKREY and his group, on diethylnitrosamine and 4-dimethylaminostilbene, are described. In a recent experiment, urethane was given chronically to rats and mice, at doses between 100 and 12,500 mug/kg/day. The results in rats were evaluated with the Mantel-Bryan procedure; risk estimates greatly differed, depending on the slope used for calculation.", "contents": "Some examples of dose-response studies in chemical carcinogenesis. Three studies of DRUCKREY and his group, on diethylnitrosamine and 4-dimethylaminostilbene, are described. In a recent experiment, urethane was given chronically to rats and mice, at doses between 100 and 12,500 mug/kg/day. The results in rats were evaluated with the Mantel-Bryan procedure; risk estimates greatly differed, depending on the slope used for calculation.", "PMID": 980371}
{"id": "pubmed23n0032_12399", "title": "Repair excision of alkylated bases from DNA in vivo.", "content": "Recent experiments are reviewed which indicate that O6-alkylation of guanine in nuclear DNA constitutes a promutagenic lesion possibly implicated in malignant transformation by monofunctional alkylating carcinogens. The differential capacity of various organs to enzymically excise O6-alkylguanine from their DNA seems to correlate with the organ specificity of the carcinogenic effect.", "contents": "Repair excision of alkylated bases from DNA in vivo. Recent experiments are reviewed which indicate that O6-alkylation of guanine in nuclear DNA constitutes a promutagenic lesion possibly implicated in malignant transformation by monofunctional alkylating carcinogens. The differential capacity of various organs to enzymically excise O6-alkylguanine from their DNA seems to correlate with the organ specificity of the carcinogenic effect.", "PMID": 980372}
{"id": "pubmed23n0032_12400", "title": "Technique of combined lid and medial canthus reconstruction.", "content": "Standard lid reconstruction techniques (sliding flaps or tarsoconjunctival grafts) may be combined with the Laissez-Faire treatment of large medial canthal defects. Using this methods, satisfactory results were achieved in three cases in which it was necessary to excise both a large portion of an eyelid and the adjacent medial canthus.", "contents": "Technique of combined lid and medial canthus reconstruction. Standard lid reconstruction techniques (sliding flaps or tarsoconjunctival grafts) may be combined with the Laissez-Faire treatment of large medial canthal defects. Using this methods, satisfactory results were achieved in three cases in which it was necessary to excise both a large portion of an eyelid and the adjacent medial canthus.", "PMID": 980373}
{"id": "pubmed23n0032_12401", "title": "The management of early lacrimal tube complications.", "content": "The introduction of a closed loop of suture material through the Jones lacrimal tube, the DCR opening, and the nose at the time of surgery, facilitates the management of early postoperative complications in conjunctivo-dacrocystorhinostomy.", "contents": "The management of early lacrimal tube complications. The introduction of a closed loop of suture material through the Jones lacrimal tube, the DCR opening, and the nose at the time of surgery, facilitates the management of early postoperative complications in conjunctivo-dacrocystorhinostomy.", "PMID": 980374}
{"id": "pubmed23n0032_12402", "title": "Traumatic hyphema: treatment of secondary hemorrhage with cyclodiathermy.", "content": "Four patients were treated with diathermy applications to the ciliary body for recurrent hemorrhage and elevated intraocular pressure following traumatic hyphema. In all cases, the hemorrhage and intraocular pressure were controlled without major complications. Indications for use of diathermy include a patient with recurrent secondary hemorrhage or a clotted hyphema, with elevated intraocular pressure or corneal blood-staining, in whom a site of bleeding can be identified during surgical intervention.", "contents": "Traumatic hyphema: treatment of secondary hemorrhage with cyclodiathermy. Four patients were treated with diathermy applications to the ciliary body for recurrent hemorrhage and elevated intraocular pressure following traumatic hyphema. In all cases, the hemorrhage and intraocular pressure were controlled without major complications. Indications for use of diathermy include a patient with recurrent secondary hemorrhage or a clotted hyphema, with elevated intraocular pressure or corneal blood-staining, in whom a site of bleeding can be identified during surgical intervention.", "PMID": 980375}
{"id": "pubmed23n0032_12403", "title": "The prognosis of penetrating keratoplasty based upon corneal morphology.", "content": "In this paper, the author has attempted to express the prognosis for successful penetrating keratoplasty based upon a morphological classification of corneal disease. Four groups have been designated with their basic prognosis ranging from excellent to poor. The importance of appraising the diseased cornea in the area of its optimal recipient zone has been stressed. Ancillary factors adversely affecting the estimated final prognosis of keratoplasty have been considered, with emphasis upon the importance of glaucoma, lid function, tear film, ocular inflammation and patient compliance. Not only is it important for the surgeon to appraise the chance of successful surgery, but it is essential for the patient to be informed in advance regarding the probability for successful surgery and the need to comply with sometimes demanding requirements of postoperative care.", "contents": "The prognosis of penetrating keratoplasty based upon corneal morphology. In this paper, the author has attempted to express the prognosis for successful penetrating keratoplasty based upon a morphological classification of corneal disease. Four groups have been designated with their basic prognosis ranging from excellent to poor. The importance of appraising the diseased cornea in the area of its optimal recipient zone has been stressed. Ancillary factors adversely affecting the estimated final prognosis of keratoplasty have been considered, with emphasis upon the importance of glaucoma, lid function, tear film, ocular inflammation and patient compliance. Not only is it important for the surgeon to appraise the chance of successful surgery, but it is essential for the patient to be informed in advance regarding the probability for successful surgery and the need to comply with sometimes demanding requirements of postoperative care.", "PMID": 980376}
{"id": "pubmed23n0032_12404", "title": "Intraocular irritation evaluation of benzalkonium chloride in rabbits.", "content": "The purpose of this study was to define the threshold for intraocular irritation of benzalkonium chloride, a preservative used in some formulations which enter the anterior segment of the eye during ocular surgery. Various concentrations of benzalkonium were injected into anterior chambers of albino rabbit eyes. Conjunctivitis, flare, iritis, and corneal changes occurred in a dose response pattern. The threshold of irritation was 0.03%, with highest nonirritating concentration being 0.01%. In other works in this laboratory, threshold of irritation for topical ocular benzalkonium was 0.05%, but the nature of ocular changes was less substantial than those observed intraocularly. Because the threshold for intraocular irritation is less than that topically, the nature of ocular changes was different for two routes, and there is a paucity of clinical data for intraocular benzalkonium chloride, a safety factor of 10 was utilized in setting the highest safe concentration of 0.001% for intraocular use. The preservative efficacy of 0.001% is questionable; therefore, we cannot endorse benzalkonium chloride as a preservative for formulations which will enter the anterior segment of the eye.", "contents": "Intraocular irritation evaluation of benzalkonium chloride in rabbits. The purpose of this study was to define the threshold for intraocular irritation of benzalkonium chloride, a preservative used in some formulations which enter the anterior segment of the eye during ocular surgery. Various concentrations of benzalkonium were injected into anterior chambers of albino rabbit eyes. Conjunctivitis, flare, iritis, and corneal changes occurred in a dose response pattern. The threshold of irritation was 0.03%, with highest nonirritating concentration being 0.01%. In other works in this laboratory, threshold of irritation for topical ocular benzalkonium was 0.05%, but the nature of ocular changes was less substantial than those observed intraocularly. Because the threshold for intraocular irritation is less than that topically, the nature of ocular changes was different for two routes, and there is a paucity of clinical data for intraocular benzalkonium chloride, a safety factor of 10 was utilized in setting the highest safe concentration of 0.001% for intraocular use. The preservative efficacy of 0.001% is questionable; therefore, we cannot endorse benzalkonium chloride as a preservative for formulations which will enter the anterior segment of the eye.", "PMID": 980377}
{"id": "pubmed23n0032_12405", "title": "Phacoanaphylactic endophthalmitis: echographic diagnosis of phacoanaphylactic endophthalmitis.", "content": "A sixty-year-old black diabetic female developed a severe postoperative endophthalmitis eight days after unplanned extracapsular cataract surgery. Gram stain of a diagnostic vitreous aspirate showed no organisms, and culture of the aspirate was negative for bacteria and fungi. A- and B-scan ultrasonography showed the presence of intra-vitreal lens fragments; the diagnosis of phacoanaphylactic endophthalmitis was made and intensive steroid therapy was instituted early in the course of the disease. Resorption of the lens fragments in association with gradual diminution of the inflammatory process was documented by serial echograms of the eye. The vitreous ultimately cleared and the patient regained useful vision. Ultrasonography is recommended as an essential part of the diagnostic evaluation of any patient with postoperative endophthalmitis especially with a history of extracapsular surgery.", "contents": "Phacoanaphylactic endophthalmitis: echographic diagnosis of phacoanaphylactic endophthalmitis. A sixty-year-old black diabetic female developed a severe postoperative endophthalmitis eight days after unplanned extracapsular cataract surgery. Gram stain of a diagnostic vitreous aspirate showed no organisms, and culture of the aspirate was negative for bacteria and fungi. A- and B-scan ultrasonography showed the presence of intra-vitreal lens fragments; the diagnosis of phacoanaphylactic endophthalmitis was made and intensive steroid therapy was instituted early in the course of the disease. Resorption of the lens fragments in association with gradual diminution of the inflammatory process was documented by serial echograms of the eye. The vitreous ultimately cleared and the patient regained useful vision. Ultrasonography is recommended as an essential part of the diagnostic evaluation of any patient with postoperative endophthalmitis especially with a history of extracapsular surgery.", "PMID": 980378}
{"id": "pubmed23n0032_12406", "title": "Unusual presentation of phacoanaphylaxis following phacoemulsification.", "content": "A case of phacoanaphylaxis following phacoemulsification is presented. The unusual features of the disease included the markedly delayed onset and the clinical appearance of the hypopyon. Emphasis is placed on the cytologic examination of anterior chamber aspirate in such cases.", "contents": "Unusual presentation of phacoanaphylaxis following phacoemulsification. A case of phacoanaphylaxis following phacoemulsification is presented. The unusual features of the disease included the markedly delayed onset and the clinical appearance of the hypopyon. Emphasis is placed on the cytologic examination of anterior chamber aspirate in such cases.", "PMID": 980379}
{"id": "pubmed23n0032_12407", "title": "Pitfalls in the determination of intraocular lens power without ultrasound.", "content": "The rule is simple: An 18 diopter intraocular lens tends to restore the basic refraction. For each diopter of basic refraction to be corrected add or subtract 1.25 diopters. To determine the basic refraction a careful history and an examination of old glasses and past refraction records is necessary. When the basic refraction cannot be ascertained, capital errors may occur. Given the basic refraction, errors or several diopters are still apt to occur. Calculation of intraocular lens power based on K-readings and ultrasonic measurements is therefore always advisable and a must when there is doubt about the basic refraction.", "contents": "Pitfalls in the determination of intraocular lens power without ultrasound. The rule is simple: An 18 diopter intraocular lens tends to restore the basic refraction. For each diopter of basic refraction to be corrected add or subtract 1.25 diopters. To determine the basic refraction a careful history and an examination of old glasses and past refraction records is necessary. When the basic refraction cannot be ascertained, capital errors may occur. Given the basic refraction, errors or several diopters are still apt to occur. Calculation of intraocular lens power based on K-readings and ultrasonic measurements is therefore always advisable and a must when there is doubt about the basic refraction.", "PMID": 980380}
{"id": "pubmed23n0032_12408", "title": "Monocular aphakia and contralateral pseudophakia.", "content": "The implanatation of an intraocular lens into the contralateral eye of non-implanted monocular aphakic patients is reviewed. The specific indications, contraindications, and advantages are discussed. The results of a series of 31 cases are presented and analyzed. The generally good results and specific advantages of this technique, when utilized under proper conditions, indicate that it merits inclusion in the ever expanding spectrum of options available to the modern cataract surgeon.", "contents": "Monocular aphakia and contralateral pseudophakia. The implanatation of an intraocular lens into the contralateral eye of non-implanted monocular aphakic patients is reviewed. The specific indications, contraindications, and advantages are discussed. The results of a series of 31 cases are presented and analyzed. The generally good results and specific advantages of this technique, when utilized under proper conditions, indicate that it merits inclusion in the ever expanding spectrum of options available to the modern cataract surgeon.", "PMID": 980381}
{"id": "pubmed23n0032_12409", "title": "The efficacy of general anesthesia for cataract surgery.", "content": "This is a report of 259 consecutive cataract extractions performed under general anesthesia over a two-year period. The age of the patients ranged from 6 to 93 with an average of 71. The purpose of this paper is to reiterate the safety of modern general anesthesia for cataract extraction in the elderly, and to demonstrate it can provide a very soft eye in a completely immobilized patient. It offers the most ideal conditions to the surgeon for lens extraction and careful wound closure without the necessity of retrobulbar block, digital massage or osmotic agents.", "contents": "The efficacy of general anesthesia for cataract surgery. This is a report of 259 consecutive cataract extractions performed under general anesthesia over a two-year period. The age of the patients ranged from 6 to 93 with an average of 71. The purpose of this paper is to reiterate the safety of modern general anesthesia for cataract extraction in the elderly, and to demonstrate it can provide a very soft eye in a completely immobilized patient. It offers the most ideal conditions to the surgeon for lens extraction and careful wound closure without the necessity of retrobulbar block, digital massage or osmotic agents.", "PMID": 980382}
{"id": "pubmed23n0032_12410", "title": "Combined cataract extraction and thermal sclerostomy versus combined cataract extraction and trabeculectomy.", "content": "We performed either a combined cataract extraction and trabeculectomy or a combined cataract extraction and thermal sclerostomy on 43 eyes. All eyes had chronic open angle glaucoma or chronic angle closure glaucoma, or a combination of the two. In our cataract/trabeculectomy group, 74% had improvement in visual acuity, 91% had normalized intraocular pressure without anti-glaucoma medications, 31% had transient postoperative hyphemas. In our cataract/thermal sclerostomy group, 61% had improvement in visual acuity, 61% had normalization of intraocular pressures without antiglaucoma medications, 17% had transient postoperative hyphemas. We could not correlate any of the complications of surgery with poor intraocular pressure results in either groups. The mechanism for the higher success rate with a combined cataract extraction and trabeculectomy as compared with a combined cataract extraction and thermal sclerostomy was not obvious after analyzing these patients.", "contents": "Combined cataract extraction and thermal sclerostomy versus combined cataract extraction and trabeculectomy. We performed either a combined cataract extraction and trabeculectomy or a combined cataract extraction and thermal sclerostomy on 43 eyes. All eyes had chronic open angle glaucoma or chronic angle closure glaucoma, or a combination of the two. In our cataract/trabeculectomy group, 74% had improvement in visual acuity, 91% had normalized intraocular pressure without anti-glaucoma medications, 31% had transient postoperative hyphemas. In our cataract/thermal sclerostomy group, 61% had improvement in visual acuity, 61% had normalization of intraocular pressures without antiglaucoma medications, 17% had transient postoperative hyphemas. We could not correlate any of the complications of surgery with poor intraocular pressure results in either groups. The mechanism for the higher success rate with a combined cataract extraction and trabeculectomy as compared with a combined cataract extraction and thermal sclerostomy was not obvious after analyzing these patients.", "PMID": 980383}
{"id": "pubmed23n0032_12411", "title": "Wide-angle cutter vitrophage.", "content": "A wide-angle cutter vitrophage facilitates removal of semi-solid or thick retropupillary membranes. The cutting orifice permits engagement of inflexible material which is then cut and removed via the instrument. The wide-angle cutter has successfully removed thick secondary membranes and expedites pars plana removal of cataracts with 2+ nuclear sclerosis.", "contents": "Wide-angle cutter vitrophage. A wide-angle cutter vitrophage facilitates removal of semi-solid or thick retropupillary membranes. The cutting orifice permits engagement of inflexible material which is then cut and removed via the instrument. The wide-angle cutter has successfully removed thick secondary membranes and expedites pars plana removal of cataracts with 2+ nuclear sclerosis.", "PMID": 980384}
{"id": "pubmed23n0032_12412", "title": "The use of solid soft silicone rubber exoplants in retinal detachment surgery.", "content": "A method of retinal detachment surgery utilizing soft solid silicone rubber exoplants is described along with its use in typical cases. Solid silicone rubber, long used as a scleral implant, is a well-tolerated exoplant scleral buckling material which has been found to provide excellent long-term results. The surgical procedure described is relatively short in duration and has a minimum of operative and postoperative complications.", "contents": "The use of solid soft silicone rubber exoplants in retinal detachment surgery. A method of retinal detachment surgery utilizing soft solid silicone rubber exoplants is described along with its use in typical cases. Solid silicone rubber, long used as a scleral implant, is a well-tolerated exoplant scleral buckling material which has been found to provide excellent long-term results. The surgical procedure described is relatively short in duration and has a minimum of operative and postoperative complications.", "PMID": 980385}
{"id": "pubmed23n0032_12413", "title": "Fundus changes in primary hyperlipaemia.", "content": "The retinal vascular change described for two relatively young men and initially diagnosed as arteritis retinalis but subsequently regarded as an analogue of retinal lipaemia stresses the importance of biochemical exploration for a possible disturbance in the fat metabolism in unclear cases of retinal vasculopathy. If a disturbance in the fat metabolism is found, rational therapy will lead to disappearance of the vascular mural changes.", "contents": "Fundus changes in primary hyperlipaemia. The retinal vascular change described for two relatively young men and initially diagnosed as arteritis retinalis but subsequently regarded as an analogue of retinal lipaemia stresses the importance of biochemical exploration for a possible disturbance in the fat metabolism in unclear cases of retinal vasculopathy. If a disturbance in the fat metabolism is found, rational therapy will lead to disappearance of the vascular mural changes.", "PMID": 980390}
{"id": "pubmed23n0032_12414", "title": "Detrimental results with the common inflared shoe: clinical questioning of a historic alignment concept.", "content": "The detrimental results of shoe use manifested both in the foot and in footwear were studied and found to support earlier views that inflared alignment is a significant factor in foot damage. Mechanical implications of alignment also confirmed our impression that the inflared alignment is a deleterious influence. Absence of conclusive physiologic evidence justifying inflaring and the death of end results from use of standard straight last footwear for comparative evaluation suggest that further inquiry regarding this feature is warranted, with view to its elimination.", "contents": "Detrimental results with the common inflared shoe: clinical questioning of a historic alignment concept. The detrimental results of shoe use manifested both in the foot and in footwear were studied and found to support earlier views that inflared alignment is a significant factor in foot damage. Mechanical implications of alignment also confirmed our impression that the inflared alignment is a deleterious influence. Absence of conclusive physiologic evidence justifying inflaring and the death of end results from use of standard straight last footwear for comparative evaluation suggest that further inquiry regarding this feature is warranted, with view to its elimination.", "PMID": 980414}
{"id": "pubmed23n0032_12415", "title": "The effectiveness of shoe modifications for intoeing.", "content": "Shoe wedges have no immediate clinically significant effect on the angle of gait in children. The circular Torqheel was found to be more effetive, but still corrected only a quarter of the apparent rotatory deformity during gait.", "contents": "The effectiveness of shoe modifications for intoeing. Shoe wedges have no immediate clinically significant effect on the angle of gait in children. The circular Torqheel was found to be more effetive, but still corrected only a quarter of the apparent rotatory deformity during gait.", "PMID": 980415}
{"id": "pubmed23n0032_12416", "title": "Metatarsus varus corrected by open wedge osteotomy of the first cuneiform bone.", "content": "The persistence of metatarsus adductus varus has been a problem in management. We have treated a series of selected patients with this problem and believe that our results have been better than with the procedure we have used in the past. Fowler has described a procedure which seems ideal for the patients in our series. Through personal communication the procedure and its application were discussed and the series was started eight years ago. Our series is small because our patients are responding to other forms of treatment at an earlier age. The few that do not respond are now considered for the operation described. The procedure is relatively simple to perform. Full correction should be obtained at the time of surgery. Casting is utilized to hold the correction and immobilize the extremity for healing. Our unsatisfactory results occurred because of errors in technique or poor selection of patients. We believe that this procedure should be considered in the older patient with metatarsus varus.", "contents": "Metatarsus varus corrected by open wedge osteotomy of the first cuneiform bone. The persistence of metatarsus adductus varus has been a problem in management. We have treated a series of selected patients with this problem and believe that our results have been better than with the procedure we have used in the past. Fowler has described a procedure which seems ideal for the patients in our series. Through personal communication the procedure and its application were discussed and the series was started eight years ago. Our series is small because our patients are responding to other forms of treatment at an earlier age. The few that do not respond are now considered for the operation described. The procedure is relatively simple to perform. Full correction should be obtained at the time of surgery. Casting is utilized to hold the correction and immobilize the extremity for healing. Our unsatisfactory results occurred because of errors in technique or poor selection of patients. We believe that this procedure should be considered in the older patient with metatarsus varus.", "PMID": 980419}
{"id": "pubmed23n0032_12417", "title": "The Stone operation: a clinical review of seven years' experience.", "content": "A series of 46 patients who underwent 78 first metatarso-phalangeal operations is reported. The procedure for the Stone operation is detailed. The Stone operation is a satisfactory procedure in the surgical treatment of hallux valgus and hallux rigidus.", "contents": "The Stone operation: a clinical review of seven years' experience. A series of 46 patients who underwent 78 first metatarso-phalangeal operations is reported. The procedure for the Stone operation is detailed. The Stone operation is a satisfactory procedure in the surgical treatment of hallux valgus and hallux rigidus.", "PMID": 980420}
{"id": "pubmed23n0032_12418", "title": "Bunionette correction by metatarsal osteotomy: preliminary report.", "content": "A preliminary report is presented of 17 patients who underwent 27 fifth metatarsal osteotomies. Oblique osteotomy without internal fixation was used. Union of the osteotomy without external immobilization occurred in 16 of the patients. Correction was effected in a case of delayed union in one foot because it was symptomatic. Improvement and normal function were the result in 26 of the 27 osteotomies. Delayed union did not seem to mean failure of relief. No painful scars occurred.", "contents": "Bunionette correction by metatarsal osteotomy: preliminary report. A preliminary report is presented of 17 patients who underwent 27 fifth metatarsal osteotomies. Oblique osteotomy without internal fixation was used. Union of the osteotomy without external immobilization occurred in 16 of the patients. Correction was effected in a case of delayed union in one foot because it was symptomatic. Improvement and normal function were the result in 26 of the 27 osteotomies. Delayed union did not seem to mean failure of relief. No painful scars occurred.", "PMID": 980421}
{"id": "pubmed23n0032_12419", "title": "Nonexcision triple arthrodesis of the foot.", "content": "Nonexcision triple arthrodesis is proposed as a simple operation to relieve pain and stabilize the hindfoot. It was successful in seven of nine patients as a salvage procedure. Its further use is recommended.", "contents": "Nonexcision triple arthrodesis of the foot. Nonexcision triple arthrodesis is proposed as a simple operation to relieve pain and stabilize the hindfoot. It was successful in seven of nine patients as a salvage procedure. Its further use is recommended.", "PMID": 980424}
{"id": "pubmed23n0032_12420", "title": "Early posterior ankle release in the treatment of congenital clubfoot.", "content": "Eighty-one patients (116 clubfeet) underwent posterior ankle release before the age of two years, following unsatisfactory responses to serial corrective casts applied according to the technique of Kite. Seventy-three per cent of these feet showed no or only mild talar flattening at an average follow-up of 7.5 years. Four years, following posterior ankle release there was a none-mild talar flattening rate of 69 per cent in this group compared to a 40 per cent none-mild rate in ankle release reduces the incidence of recurrent equinus deformity and the necessity for subsequent surgery in comparison to the results obtained with serial plaster casts or with tendo Achillis lengthening alone. Recent trends in clubfoot management have favored increasingly early operative intervention. Denham stated that \"In the infant hard tissues (bone and cartilage) should be regarded as soft, and the soft tissues (tendon and ligament) as hard.\" Our operative experience with posterior ankle release supports this philosophy and indicates that early aggressive surgical management is the treatment of choice for the resistant clubfoot.", "contents": "Early posterior ankle release in the treatment of congenital clubfoot. Eighty-one patients (116 clubfeet) underwent posterior ankle release before the age of two years, following unsatisfactory responses to serial corrective casts applied according to the technique of Kite. Seventy-three per cent of these feet showed no or only mild talar flattening at an average follow-up of 7.5 years. Four years, following posterior ankle release there was a none-mild talar flattening rate of 69 per cent in this group compared to a 40 per cent none-mild rate in ankle release reduces the incidence of recurrent equinus deformity and the necessity for subsequent surgery in comparison to the results obtained with serial plaster casts or with tendo Achillis lengthening alone. Recent trends in clubfoot management have favored increasingly early operative intervention. Denham stated that \"In the infant hard tissues (bone and cartilage) should be regarded as soft, and the soft tissues (tendon and ligament) as hard.\" Our operative experience with posterior ankle release supports this philosophy and indicates that early aggressive surgical management is the treatment of choice for the resistant clubfoot.", "PMID": 980427}
{"id": "pubmed23n0032_12421", "title": "Tibiotalar torsion: bioengineering paradigm.", "content": "1. Medial tibiotalar torsion is the most common disorder peculiar to mankind. 2. The pathogonomic findings are (a) an axial medially rotated and adducted distal third of the shaft of the tibia, (b) the plafond of the tibia with its mortise containing the \"track-bound\" talus, which is deflected strongly toward the tibial side, (c) an exaggerated midtarsal equinus, (d) ostensible restriction of dorsiflexion of the hindfoot against the tibia, (e) mild separation of the distal tibiofibular articulation, and (f) forward displacement of the gravitational axis to the naviculocunei-form joint. 3. Faulty leg crossing in utero resulting in an abnormal pelvofemoral-tibial design is discussed and its important consequences in the vulnerable 40 per cent of the population are emphasized. 4. The kinesiomechanics of the leg, ankle and foot is reviewed. 5. The radiographic parameters of medial tibiotalar torsion are presented, as well as the multiple facets of the clinical examination. 6. Methods of treatment depending on age and severity of the disorder are recommended. Surgery, detortional casts, and corrective footwear are discussed. Shoes presently available are inadequate for tibiotalar torsion and therefore engineering principles must be applied in the design and construction of all footwear, including sneakers and sportswear. This can be done only if the pathological biomechanics of this group of disorders is recognized. Biplane proximal tibial osteotomy is recommended in refractory cases, especially when tibiotalar torsion is demonstrated. 7. After 30 years of experience, the author finds that results with these patients have been uniformly good to excellent, depending on age and mode of treatment. 8. In medial tibiotalar torsion, the consequent adaptive changes are readily observed, but rarely are they recognized as the inevitable sequelae of medial tibiotalar torsion. 9. Adaptive compensating disorders are identified and their mechanism described. 10. The management of torsional and static disorders and adaptations of the leg, foot, and knee should not continue on an empirical basis. Recognition of recently presented parameters of the lower limbs in the vulnerable \"40 percenters\" permits the use of sound engineering principles in both conservative and operative correction of these disorders.", "contents": "Tibiotalar torsion: bioengineering paradigm. 1. Medial tibiotalar torsion is the most common disorder peculiar to mankind. 2. The pathogonomic findings are (a) an axial medially rotated and adducted distal third of the shaft of the tibia, (b) the plafond of the tibia with its mortise containing the \"track-bound\" talus, which is deflected strongly toward the tibial side, (c) an exaggerated midtarsal equinus, (d) ostensible restriction of dorsiflexion of the hindfoot against the tibia, (e) mild separation of the distal tibiofibular articulation, and (f) forward displacement of the gravitational axis to the naviculocunei-form joint. 3. Faulty leg crossing in utero resulting in an abnormal pelvofemoral-tibial design is discussed and its important consequences in the vulnerable 40 per cent of the population are emphasized. 4. The kinesiomechanics of the leg, ankle and foot is reviewed. 5. The radiographic parameters of medial tibiotalar torsion are presented, as well as the multiple facets of the clinical examination. 6. Methods of treatment depending on age and severity of the disorder are recommended. Surgery, detortional casts, and corrective footwear are discussed. Shoes presently available are inadequate for tibiotalar torsion and therefore engineering principles must be applied in the design and construction of all footwear, including sneakers and sportswear. This can be done only if the pathological biomechanics of this group of disorders is recognized. Biplane proximal tibial osteotomy is recommended in refractory cases, especially when tibiotalar torsion is demonstrated. 7. After 30 years of experience, the author finds that results with these patients have been uniformly good to excellent, depending on age and mode of treatment. 8. In medial tibiotalar torsion, the consequent adaptive changes are readily observed, but rarely are they recognized as the inevitable sequelae of medial tibiotalar torsion. 9. Adaptive compensating disorders are identified and their mechanism described. 10. The management of torsional and static disorders and adaptations of the leg, foot, and knee should not continue on an empirical basis. Recognition of recently presented parameters of the lower limbs in the vulnerable \"40 percenters\" permits the use of sound engineering principles in both conservative and operative correction of these disorders.", "PMID": 980429}
{"id": "pubmed23n0032_12422", "title": "Medial subtalar stabilization with posterior medial release in the treatment of varus feet: a preliminary report.", "content": "The problem of the varus hindfoot in children ages six to 10 has been discussed. A preliminary follow-up is presented on six patients treated by extensive medial release, multiple tendon lengthenings, and subtalar intra-articular arthrodesis. Two of our patients revealed incomplete correction of the deformity because of technical errors in the medial release. However, there have been no failures of fusion and no progression of the deformity to date. This is a preliminary report and further follow-up is indicated. It should be noted that we have now extended our series to 40 patients.", "contents": "Medial subtalar stabilization with posterior medial release in the treatment of varus feet: a preliminary report. The problem of the varus hindfoot in children ages six to 10 has been discussed. A preliminary follow-up is presented on six patients treated by extensive medial release, multiple tendon lengthenings, and subtalar intra-articular arthrodesis. Two of our patients revealed incomplete correction of the deformity because of technical errors in the medial release. However, there have been no failures of fusion and no progression of the deformity to date. This is a preliminary report and further follow-up is indicated. It should be noted that we have now extended our series to 40 patients.", "PMID": 980430}
{"id": "pubmed23n0032_12423", "title": "Hemophilic arthropathy of the foot and ankle.", "content": "The major deformities in hemophilic arthropathy of the foot and ankle fall into the three groups of equinus, varus, and cavus. These pathologic positions develop through a repetitive pattern of intra-articular and intramuscular bleeding within the area of the distal calf, foot, and ankle. Appropriate infusion therapy with factor VIII concentrate and factor IX concentrate plus splinting, bracing, and logical rehabilitative maneuvers can delay or prevent the advent of permanent deformity. The actual articular damage seems to be directly related to the release of digestive enzymes from leukocytes, synovial cells, and other blood products. These pathologic mechanisms appear to be similar to those recently described in explaining joint destruction in rheumatoid and degenerative arthritis. The indication for certain reconstructive orthopedic procedures in these situations are given and case examples provided. Total joint replacement in the area of the foot and ankle in hemophilia has been considered for certain patients by the authors but not attempted as yet.", "contents": "Hemophilic arthropathy of the foot and ankle. The major deformities in hemophilic arthropathy of the foot and ankle fall into the three groups of equinus, varus, and cavus. These pathologic positions develop through a repetitive pattern of intra-articular and intramuscular bleeding within the area of the distal calf, foot, and ankle. Appropriate infusion therapy with factor VIII concentrate and factor IX concentrate plus splinting, bracing, and logical rehabilitative maneuvers can delay or prevent the advent of permanent deformity. The actual articular damage seems to be directly related to the release of digestive enzymes from leukocytes, synovial cells, and other blood products. These pathologic mechanisms appear to be similar to those recently described in explaining joint destruction in rheumatoid and degenerative arthritis. The indication for certain reconstructive orthopedic procedures in these situations are given and case examples provided. Total joint replacement in the area of the foot and ankle in hemophilia has been considered for certain patients by the authors but not attempted as yet.", "PMID": 980433}
{"id": "pubmed23n0032_12424", "title": "Human milk lipases and their possible role in fat digestion.", "content": "Human milk contains two lipases. One is a lipoprotein lipase with properties similar to the lipoprotein lipases that participate in the metabolism of blood plasma lipoproteins in several tissues. This enzyme is present in high activity in the lactating mammary gland where it facilitates the uptake of triglyceride fatty acids from the blood lipoproteins for production of milk lipids in the gland. The high activity of this enzyme in milk probably represent leakage of enzyme from the gland. This lipase is not stable at pH below 5 or in intestinal contents and it is unlikely that it participates in intestinal fat digestion. Its activity varies widely between individual milk samples, and there is a high correlation between its activity and the development of hydrolytic rancidity in the milk on storage. The other lipase is present in the milk in an inactive form which is activated by bile salts. This lipase is present in milk from primates but not in milk from lower animals. Human milk contains enough of this lipase to hydrolyze the milk lipids almost completely in less than half an hour at the pH and the bile acid and salt concentrations found in the small intestine of the human infant. It is probable that it increases the efficiency of milk fat absorption. The enzyme has a rather wide substrate specificity and may also act on other lipid substrates than triglycerides. In contrast to pancreatic lipase it hydrolyses all three ester bonds in a triglyceride. This may affect the physical chemistry of the lipids in the intestinal contents as well as their absorption and further metabolism in the musoca.", "contents": "Human milk lipases and their possible role in fat digestion. Human milk contains two lipases. One is a lipoprotein lipase with properties similar to the lipoprotein lipases that participate in the metabolism of blood plasma lipoproteins in several tissues. This enzyme is present in high activity in the lactating mammary gland where it facilitates the uptake of triglyceride fatty acids from the blood lipoproteins for production of milk lipids in the gland. The high activity of this enzyme in milk probably represent leakage of enzyme from the gland. This lipase is not stable at pH below 5 or in intestinal contents and it is unlikely that it participates in intestinal fat digestion. Its activity varies widely between individual milk samples, and there is a high correlation between its activity and the development of hydrolytic rancidity in the milk on storage. The other lipase is present in the milk in an inactive form which is activated by bile salts. This lipase is present in milk from primates but not in milk from lower animals. Human milk contains enough of this lipase to hydrolyze the milk lipids almost completely in less than half an hour at the pH and the bile acid and salt concentrations found in the small intestine of the human infant. It is probable that it increases the efficiency of milk fat absorption. The enzyme has a rather wide substrate specificity and may also act on other lipid substrates than triglycerides. In contrast to pancreatic lipase it hydrolyses all three ester bonds in a triglyceride. This may affect the physical chemistry of the lipids in the intestinal contents as well as their absorption and further metabolism in the musoca.", "PMID": 980524}
{"id": "pubmed23n0032_12425", "title": "[Diagnosis and treatment of renal traumas in childhood (author's transl)].", "content": "The diagnosis of a kidney trauma is discussed and the great importance of angiography besides i. v. urography and retrograde pyelogram is pointed out. In surgical treatment exact urinary diversion and wound drainage are most important points to avoid later complications.", "contents": "[Diagnosis and treatment of renal traumas in childhood (author's transl)]. The diagnosis of a kidney trauma is discussed and the great importance of angiography besides i. v. urography and retrograde pyelogram is pointed out. In surgical treatment exact urinary diversion and wound drainage are most important points to avoid later complications.", "PMID": 980525}
{"id": "pubmed23n0032_12426", "title": "[Infectious skin disease in the newborn (author's transl)].", "content": "The most important infectious skin diseases of the newbornperiod are discussed in a short survey. Those skin alterations which can already be observed immediately after birth are especially emphasized. The search for skin infections has to be a routine measure in the care of the newborn, because even the smallest pyoderms can lead to septic complications, and non-infected newborn must be protected through the quarantining and treatment of those newborns with the infection. Frequent occurrence of pyoderms should be taken as a reason for examining the hygienic measures in the delivery room, nursery and maternity ward.", "contents": "[Infectious skin disease in the newborn (author's transl)]. The most important infectious skin diseases of the newbornperiod are discussed in a short survey. Those skin alterations which can already be observed immediately after birth are especially emphasized. The search for skin infections has to be a routine measure in the care of the newborn, because even the smallest pyoderms can lead to septic complications, and non-infected newborn must be protected through the quarantining and treatment of those newborns with the infection. Frequent occurrence of pyoderms should be taken as a reason for examining the hygienic measures in the delivery room, nursery and maternity ward.", "PMID": 980526}
{"id": "pubmed23n0032_12427", "title": "[Hemangioma simplex of the larynx in an infant (author's transl)].", "content": "Symptoms of inspiratory stridor, dyspnoa and cyanosis appeared in a four month old boy. After the direct laryngoscopy and biopsy a hemangioma simples of the larynx was diagnosed. The tumor itself was successfully treated by cryosurgery. The advantages of this therapy were pointed out and the cryosurgery can be recommended in cases of larynxangioma.", "contents": "[Hemangioma simplex of the larynx in an infant (author's transl)]. Symptoms of inspiratory stridor, dyspnoa and cyanosis appeared in a four month old boy. After the direct laryngoscopy and biopsy a hemangioma simples of the larynx was diagnosed. The tumor itself was successfully treated by cryosurgery. The advantages of this therapy were pointed out and the cryosurgery can be recommended in cases of larynxangioma.", "PMID": 980527}
{"id": "pubmed23n0032_12428", "title": "Drug screening in cell culture for the detection of anticoccidial activity.", "content": "Methods for screening compounds against Eimeria tenella in cell culture and in the chicken are described. An analysis for incidence of anticoccidial activity and host cell toxicity has been made of 11550 compounds screened in vitro, and some correlation of activity in vitro and in vivo has been attempted. The results show that screening compounds in tissue (cell) culture is not a satisfactory or reliable alternative to screening in chickens, although in vitro studies undoubtedly have a part to play in the evaluation of an active drug.", "contents": "Drug screening in cell culture for the detection of anticoccidial activity. Methods for screening compounds against Eimeria tenella in cell culture and in the chicken are described. An analysis for incidence of anticoccidial activity and host cell toxicity has been made of 11550 compounds screened in vitro, and some correlation of activity in vitro and in vivo has been attempted. The results show that screening compounds in tissue (cell) culture is not a satisfactory or reliable alternative to screening in chickens, although in vitro studies undoubtedly have a part to play in the evaluation of an active drug.", "PMID": 980538}
{"id": "pubmed23n0032_12429", "title": "Molluscs interfering with the capacity of Fasciola hepatica miracidia to infect Lymnaea truncatula.", "content": "Fasciola hepatica miracidia labelled with radioselenium were used to study aspects of their host-finding capacity by determining radioactivity subsequently taken up by exposed 'target' snails (Lymnaea truncatula). Possible interfering effects exercised by a number of non-host snails and bivalves were examined in linear test channels. The infection rate (radioactivity) among 'target' snails was markedly lowered when non-host Lymnaea species (L. pereger, L. palustris, L. stagnalis) were interposed as 'decoys'. The prosobranch Bithynia tentaculata and the bivalve Sphaerium corneum exhibited a slight decoy effect whereas pulmonate species like Anisus vortex, Gyraulus albus, Planorbis planorbis, Physa fontinalis did not interfere with miracidial host-finding. Other experiments showed that miracidia are more strongly attracted towards L. truncatula than L. pereger. Miracidia are not able to penetrate intact egg clusters of L. truncatula.", "contents": "Molluscs interfering with the capacity of Fasciola hepatica miracidia to infect Lymnaea truncatula. Fasciola hepatica miracidia labelled with radioselenium were used to study aspects of their host-finding capacity by determining radioactivity subsequently taken up by exposed 'target' snails (Lymnaea truncatula). Possible interfering effects exercised by a number of non-host snails and bivalves were examined in linear test channels. The infection rate (radioactivity) among 'target' snails was markedly lowered when non-host Lymnaea species (L. pereger, L. palustris, L. stagnalis) were interposed as 'decoys'. The prosobranch Bithynia tentaculata and the bivalve Sphaerium corneum exhibited a slight decoy effect whereas pulmonate species like Anisus vortex, Gyraulus albus, Planorbis planorbis, Physa fontinalis did not interfere with miracidial host-finding. Other experiments showed that miracidia are more strongly attracted towards L. truncatula than L. pereger. Miracidia are not able to penetrate intact egg clusters of L. truncatula.", "PMID": 980539}
{"id": "pubmed23n0032_12430", "title": "Schistosoma mansoni: the occurrence of microvilli on the surface of the tegument during transformation from cercaria to schistosomulum.", "content": "Microvilli are developed on the surface of Schistosoma mansoni schistosomula during penetration of the host skin; they form rapidly but are lost approximately 90 min after penetration. Identical microvilli are also formed on schistosomula which have penetrated a mouse skin preparation in vitro, and on schistosomula prepared by mechanical separation of the tail from the body of the cercaria. The microvilli, which are limited by the trilaminate tegumental membrane of the cercaria, eventually degenerate and are cast off from the surface of the tegument. There is little change in the surface area of the schistosomulum at this time, and the formation and loss of microvilli coincides with the replacement of the cercarial tegumental membrane by the new heptalaminate membrane. It is suggested that during the cercaria/schistosomulum transformation, some intramembraneous components of the original cercarial membran e may migrate into the new heptalaminate membrane and thus be retained, while other peripheral components such as the glycocalyx are almost certainly lost.", "contents": "Schistosoma mansoni: the occurrence of microvilli on the surface of the tegument during transformation from cercaria to schistosomulum. Microvilli are developed on the surface of Schistosoma mansoni schistosomula during penetration of the host skin; they form rapidly but are lost approximately 90 min after penetration. Identical microvilli are also formed on schistosomula which have penetrated a mouse skin preparation in vitro, and on schistosomula prepared by mechanical separation of the tail from the body of the cercaria. The microvilli, which are limited by the trilaminate tegumental membrane of the cercaria, eventually degenerate and are cast off from the surface of the tegument. There is little change in the surface area of the schistosomulum at this time, and the formation and loss of microvilli coincides with the replacement of the cercarial tegumental membrane by the new heptalaminate membrane. It is suggested that during the cercaria/schistosomulum transformation, some intramembraneous components of the original cercarial membran e may migrate into the new heptalaminate membrane and thus be retained, while other peripheral components such as the glycocalyx are almost certainly lost.", "PMID": 980540}
{"id": "pubmed23n0032_12431", "title": "Seasonal abundance of Proteocephalus ambloplitis (Cestoidea: Proteocephalidea) from largemouth bass living in a heated reservoir.", "content": "Proteocephalus ambloplitis was recovered from largemouth bass inhabiting different thermal regimes, ranging from normal to greater than 10 degrees C above normal, on the Energy Research and Development Administration's (ERDA) Savannah River Plant near Aiken, South Carolina. Possible alterations in the mechanisms initiating plerocercoid migration as proposed by Fischer & Freeman (1969) were noted. According to these authors, a rise in temperature from 4 to 7 degrees C and above stimulated migration of parenteric plerocercoids into the gut. The lowest water temperature recorded for the reservoir for the entire sampling period was 8.0 degrees C. Theoretically, this should have terminated the life-cycle of this tapeworm since migration temperature was never attained. At Savannah River, the appearance of adult tapeworms coincided with a decrease in water temperature, while the appearance of adult tapeworms in Canada was correlated with an increase in water temperature. It is postulated that the decline in water temperature in southern latitudes and the increase in water temperature in northern latitudes initiates the same response, that is, migration of plerocercoids from parenteric to enteric sites where maturation to the adult form ensues.", "contents": "Seasonal abundance of Proteocephalus ambloplitis (Cestoidea: Proteocephalidea) from largemouth bass living in a heated reservoir. Proteocephalus ambloplitis was recovered from largemouth bass inhabiting different thermal regimes, ranging from normal to greater than 10 degrees C above normal, on the Energy Research and Development Administration's (ERDA) Savannah River Plant near Aiken, South Carolina. Possible alterations in the mechanisms initiating plerocercoid migration as proposed by Fischer & Freeman (1969) were noted. According to these authors, a rise in temperature from 4 to 7 degrees C and above stimulated migration of parenteric plerocercoids into the gut. The lowest water temperature recorded for the reservoir for the entire sampling period was 8.0 degrees C. Theoretically, this should have terminated the life-cycle of this tapeworm since migration temperature was never attained. At Savannah River, the appearance of adult tapeworms coincided with a decrease in water temperature, while the appearance of adult tapeworms in Canada was correlated with an increase in water temperature. It is postulated that the decline in water temperature in southern latitudes and the increase in water temperature in northern latitudes initiates the same response, that is, migration of plerocercoids from parenteric to enteric sites where maturation to the adult form ensues.", "PMID": 980541}
{"id": "pubmed23n0032_12432", "title": "Response of Haemonchus contortus cayugensis to a change in the ratio of smooth to linguiform.", "content": "The equilibrium of vulvar phenotypes in Haemonchus contortus cayugensis was changed by infecting lambs with either a selected strain of smooth or of linguiform worms. Subsequently the lambs grazed the same pasture and observations were made on changes in worm populations in the lambs, and on pasture it appeared that the seasonal change in the relative frequencies of A and B linguiform worms was related to density-dependent factors in the host. In the spring, when lambs first become infected, the worm population is small and the majority of linguiform worms are A-type. As the season progresses the density of the parasite population increases. Once the population becomes crowded selection begins to favour the B-type females. The effects of crowding on linguiform A females can be seen as a positive correlation between the percentage of linguiform A and their weight. Population density also affected the survival of A-type worms since there was a negative correlation of percentage A-type on total worm numbers. There was a similar negative correlation between percentage A-type on total worm numbers. There was a similar negative correlation between percentage A-type and total worm numbers in both the continuously infected and tracer lambs. Population density did not affect the proportion of smooth worms. However, smooth worms increased in the tracers from approximately 10% at the beginning of the experiment to 55% at its conclusion 18 weeks later. The latter percentage is almost within range of the local subspecies, which has 60% or more smooth worms. It appears therefore that the proportion of smooth to linguiform worms is a stable equilibrium maintained by natural selection.", "contents": "Response of Haemonchus contortus cayugensis to a change in the ratio of smooth to linguiform. The equilibrium of vulvar phenotypes in Haemonchus contortus cayugensis was changed by infecting lambs with either a selected strain of smooth or of linguiform worms. Subsequently the lambs grazed the same pasture and observations were made on changes in worm populations in the lambs, and on pasture it appeared that the seasonal change in the relative frequencies of A and B linguiform worms was related to density-dependent factors in the host. In the spring, when lambs first become infected, the worm population is small and the majority of linguiform worms are A-type. As the season progresses the density of the parasite population increases. Once the population becomes crowded selection begins to favour the B-type females. The effects of crowding on linguiform A females can be seen as a positive correlation between the percentage of linguiform A and their weight. Population density also affected the survival of A-type worms since there was a negative correlation of percentage A-type on total worm numbers. There was a similar negative correlation between percentage A-type on total worm numbers. There was a similar negative correlation between percentage A-type and total worm numbers in both the continuously infected and tracer lambs. Population density did not affect the proportion of smooth worms. However, smooth worms increased in the tracers from approximately 10% at the beginning of the experiment to 55% at its conclusion 18 weeks later. The latter percentage is almost within range of the local subspecies, which has 60% or more smooth worms. It appears therefore that the proportion of smooth to linguiform worms is a stable equilibrium maintained by natural selection.", "PMID": 980542}
{"id": "pubmed23n0032_12433", "title": "Cell and organ culture techniques applied to the study of carcinoma of colon and rectum.", "content": "The value of a number of in vitro systems which have been used in the investigation of carcinomas of colon and rectum is considered. Tissue culture cell ines which have been established from human and mouse rectal and colon tumours are described. Both mouse and human cells retain ultrastructural features of the tumours of origin; acinus formations, junctional complexes, microvilli with surface glycoprotein strands. Normal colo-rectal epithelium has not been established in cell culture but organ cultures of mouse colon, which retain normal ultrastructural features can be maintained for at least 28 days. The possible origin of non-epithelial cells which appear in cultures from normal colon is discussed. The areas in which the various in vitro systems may be of used are reviewed.", "contents": "Cell and organ culture techniques applied to the study of carcinoma of colon and rectum. The value of a number of in vitro systems which have been used in the investigation of carcinomas of colon and rectum is considered. Tissue culture cell ines which have been established from human and mouse rectal and colon tumours are described. Both mouse and human cells retain ultrastructural features of the tumours of origin; acinus formations, junctional complexes, microvilli with surface glycoprotein strands. Normal colo-rectal epithelium has not been established in cell culture but organ cultures of mouse colon, which retain normal ultrastructural features can be maintained for at least 28 days. The possible origin of non-epithelial cells which appear in cultures from normal colon is discussed. The areas in which the various in vitro systems may be of used are reviewed.", "PMID": 980543}
{"id": "pubmed23n0032_12434", "title": "Metaplastic conversion of the differentiation pattern in oral epithelia affected by leukoplakia simplex. A stereologic study.", "content": "The present study applied methods of stereologic cytology to clinically specified lesions of leukoplakia simplex which originated from the oral mucous membrane at the cheek and the floor of the mouth. The data provided the following evidence: (1) The structure of and the differentiation gradients for various cytoplasmic constituents in leukoplakia simplex epithelium were identical at both sites. (2) The differentiation pattern of leukoplakic epithelia was adversely different from that of the respective normal epithelium. (3) This alteration which resulted in a striking resemblance between the leukoplakic epithelia and those of normal skin or hard palatum conceivably can be regarded as a squamous metaplasia characterized by the conversion of one differentiation pattern into another. (4) This shift in the differentiation pattern hardly could have been disclosed by means other than stereologic morphometry.", "contents": "Metaplastic conversion of the differentiation pattern in oral epithelia affected by leukoplakia simplex. A stereologic study. The present study applied methods of stereologic cytology to clinically specified lesions of leukoplakia simplex which originated from the oral mucous membrane at the cheek and the floor of the mouth. The data provided the following evidence: (1) The structure of and the differentiation gradients for various cytoplasmic constituents in leukoplakia simplex epithelium were identical at both sites. (2) The differentiation pattern of leukoplakic epithelia was adversely different from that of the respective normal epithelium. (3) This alteration which resulted in a striking resemblance between the leukoplakic epithelia and those of normal skin or hard palatum conceivably can be regarded as a squamous metaplasia characterized by the conversion of one differentiation pattern into another. (4) This shift in the differentiation pattern hardly could have been disclosed by means other than stereologic morphometry.", "PMID": 980544}
{"id": "pubmed23n0032_12435", "title": "The internal surface area of the lung in emphysema.", "content": "Quantitative histological studies on the lung are time consuming and for the pathologist may not reveal much additional imformation in relation to the time spent. Sixty-eight cases of emphysema were studied. The percentages of the lung tissue destroyed by alveolar, bronchiolar and bullous emphysema were determined by macroscopic point counting. The internal surface area (I.S.A.) and internal surface area at a standard lung volume of 3,000 ml(I.S.A.3,000) were determined by the mean linear intercept (M.L.I.) method. There was a significant correlation between both the M.L.I. and I.S.A.3,000 and the percentage of lung tissue destroyed. The M.L.I. was the most accurate guide to the presence of emphysema. The I.S.A. was the least reliable indication of emphysema. Cases of alveolar duct emphysema were both over and underdiagnosed on macroscopic point counting. It is therefore suggested that in cases with alveolar duct emphysema, apparently normal lungs or where full pulmonary function tests are available, quantitative histological studies should be carried out. In other cases it is possible to derive the I.S.A. and I.S.A.3,000 from the percentage of emphysema as determined macroscopically, without recourse to histological methods.", "contents": "The internal surface area of the lung in emphysema. Quantitative histological studies on the lung are time consuming and for the pathologist may not reveal much additional imformation in relation to the time spent. Sixty-eight cases of emphysema were studied. The percentages of the lung tissue destroyed by alveolar, bronchiolar and bullous emphysema were determined by macroscopic point counting. The internal surface area (I.S.A.) and internal surface area at a standard lung volume of 3,000 ml(I.S.A.3,000) were determined by the mean linear intercept (M.L.I.) method. There was a significant correlation between both the M.L.I. and I.S.A.3,000 and the percentage of lung tissue destroyed. The M.L.I. was the most accurate guide to the presence of emphysema. The I.S.A. was the least reliable indication of emphysema. Cases of alveolar duct emphysema were both over and underdiagnosed on macroscopic point counting. It is therefore suggested that in cases with alveolar duct emphysema, apparently normal lungs or where full pulmonary function tests are available, quantitative histological studies should be carried out. In other cases it is possible to derive the I.S.A. and I.S.A.3,000 from the percentage of emphysema as determined macroscopically, without recourse to histological methods.", "PMID": 980545}
{"id": "pubmed23n0032_12436", "title": "Gastroesophageal reflux in children: clinical manifestations, diagnosis, pathophysiology, and therapy.", "content": "Gastroesophageal reflux in infants and children is a challenging diagnostic problem. A careful history and physical examination are of foremost importance. In infants, the esophageal manometry study and the Tuttle test are helpful in confirming gastroesophageal reflux. In older children, these two studies as well as the Bernstein test should be done to document reflux. The presence of esophagitis or esophageal strictures is best determined by esophagoscopy with concomitant grasp or suction biopsies. A medical regimen should be tried for three to six weeks in all children except those with esophageal strictures or severe malnutrition. Medical failures should be treated surgically with Nissen fundoplications, performed by a competent pediatric surgeon. The prognosis for children undergoing surgical correction is excellent.", "contents": "Gastroesophageal reflux in children: clinical manifestations, diagnosis, pathophysiology, and therapy. Gastroesophageal reflux in infants and children is a challenging diagnostic problem. A careful history and physical examination are of foremost importance. In infants, the esophageal manometry study and the Tuttle test are helpful in confirming gastroesophageal reflux. In older children, these two studies as well as the Bernstein test should be done to document reflux. The presence of esophagitis or esophageal strictures is best determined by esophagoscopy with concomitant grasp or suction biopsies. A medical regimen should be tried for three to six weeks in all children except those with esophageal strictures or severe malnutrition. Medical failures should be treated surgically with Nissen fundoplications, performed by a competent pediatric surgeon. The prognosis for children undergoing surgical correction is excellent.", "PMID": 980547}
{"id": "pubmed23n0032_12437", "title": "The rate of cerebral utilization of glucose, ketone bodies, and oxygen: a comparative in vivo study of infant and adult rats.", "content": "Cerebral blood flow (CBF) was measured by means of Celabeled microspheres in infant (20-day-old) and adult (3-month-old) rats, anesthetised with Na-5-ethyl-5-(1-methylpropyl)2-thiobarbituric acid. Cerebral arteriovenous differences of acetoacetate, D-beta-hydroxybutyrate, glucose, lactate, and oxygen and brain DNA content were determined in other groups of similarly treated infant and adult animals fed or starved for 48 or 72 hr. The mean CBF values of 0.48+/-0.04 and 0.62+/-0.07 ml/(g X min), +/- SEM, in infant and adult animals, respectively, were not significantly different. CBF was unaffected by starvation. At any given arterial concentration the cerebral arteriovenous difference of acetoacetate was significantly higher in infant than adult rats. The same was true for D-beta-hydroxybutyrate at arterial concentrations above 1 mmol/liter. There was an approximately linear relationship between arterial concentration of acetoacetate and its cerebral arteriovenous difference in both infant and adult rats. A similar relationship was found for D-beta-hydroxybutyrate only in infant animals. In the fed state, the cerebral uptake of glucose and ketone bodies (micromoles per (mg DNA X min)) was not different in infant and adult rats. During starvation, cerebral uptake of ketone bodies expressed as micromoles per (mg DNA X min) was higher in infant than adult rats, indicating a higher rate of utilization of ketone bodies per cell in these animals. For glucose, no such difference was found in either fed or starved groups (Table 3). The average percentage of the total cerebral uptake of substrates (micromoles per min) accounted for by ketone bodies increased in both infant and adult rats during starvation. This percentage value was clearly higher in infant than adult rats during starvation. After 72 hr of starvation the values were 38.8% and 15.2% in infant and adult rats, respectively (Fig. 3). Calculated cerebral metabolic rate for oxygen (CMRO2), assuming complete oxidation of glucose and ketone bodies and expressed as micromoles per (mg DNA X min), was similar in fed and starved rats of both age groups (Table 3), indicating that ketone bodies serve as an alternative substrate for glucose during starvation. Calculated CMRO2 for glucose plus ketone bodies was similar to the measured CMRO2 in adult rats both in the fed and the starved groups. For infant rats, calculated CMRO2 for glucose plus ketone bodies was higher than measured CMRO2, indicating that in this age group a portion of substrate was used for synthesis or storage rather than for complete oxidation.", "contents": "The rate of cerebral utilization of glucose, ketone bodies, and oxygen: a comparative in vivo study of infant and adult rats. Cerebral blood flow (CBF) was measured by means of Celabeled microspheres in infant (20-day-old) and adult (3-month-old) rats, anesthetised with Na-5-ethyl-5-(1-methylpropyl)2-thiobarbituric acid. Cerebral arteriovenous differences of acetoacetate, D-beta-hydroxybutyrate, glucose, lactate, and oxygen and brain DNA content were determined in other groups of similarly treated infant and adult animals fed or starved for 48 or 72 hr. The mean CBF values of 0.48+/-0.04 and 0.62+/-0.07 ml/(g X min), +/- SEM, in infant and adult animals, respectively, were not significantly different. CBF was unaffected by starvation. At any given arterial concentration the cerebral arteriovenous difference of acetoacetate was significantly higher in infant than adult rats. The same was true for D-beta-hydroxybutyrate at arterial concentrations above 1 mmol/liter. There was an approximately linear relationship between arterial concentration of acetoacetate and its cerebral arteriovenous difference in both infant and adult rats. A similar relationship was found for D-beta-hydroxybutyrate only in infant animals. In the fed state, the cerebral uptake of glucose and ketone bodies (micromoles per (mg DNA X min)) was not different in infant and adult rats. During starvation, cerebral uptake of ketone bodies expressed as micromoles per (mg DNA X min) was higher in infant than adult rats, indicating a higher rate of utilization of ketone bodies per cell in these animals. For glucose, no such difference was found in either fed or starved groups (Table 3). The average percentage of the total cerebral uptake of substrates (micromoles per min) accounted for by ketone bodies increased in both infant and adult rats during starvation. This percentage value was clearly higher in infant than adult rats during starvation. After 72 hr of starvation the values were 38.8% and 15.2% in infant and adult rats, respectively (Fig. 3). Calculated cerebral metabolic rate for oxygen (CMRO2), assuming complete oxidation of glucose and ketone bodies and expressed as micromoles per (mg DNA X min), was similar in fed and starved rats of both age groups (Table 3), indicating that ketone bodies serve as an alternative substrate for glucose during starvation. Calculated CMRO2 for glucose plus ketone bodies was similar to the measured CMRO2 in adult rats both in the fed and the starved groups. For infant rats, calculated CMRO2 for glucose plus ketone bodies was higher than measured CMRO2, indicating that in this age group a portion of substrate was used for synthesis or storage rather than for complete oxidation.", "PMID": 980550}
{"id": "pubmed23n0032_12438", "title": "Ornithine transcarbamylase deficiency: enzyme studies on a further case and a method of diagnosis using plasma enzyme ratios.", "content": "The activities of the urea cycle enzymes in the liver of a female patient with hyperammonemia were determined (Table 1). Ornithine transcarbamylase (OTC, EC. 2.1.3.3) was reduced to 5-10% of normal and the residual enzyme showed an apparent Kmorn of 0.69 (normal 0.37 +/- 0.10) mmol liter. The pH dependence was normal. The patient's mother also showed hyperammonemia but was not clinically affected. Consideration of the genetics of the disease suggested that many female patients should have a mixture of normal and mutant enzymes. Electrophoresis of the patient's liver extract showed an additional band of OTC activity probably due to this mutant enzyme. The ratio of plasma glutamate-pyruvate transaminase to OTC was abnormal in four clinically affected patients with OTC deficiency (Fig. 4B) but not in two of their mothers without clinical signs.", "contents": "Ornithine transcarbamylase deficiency: enzyme studies on a further case and a method of diagnosis using plasma enzyme ratios. The activities of the urea cycle enzymes in the liver of a female patient with hyperammonemia were determined (Table 1). Ornithine transcarbamylase (OTC, EC. 2.1.3.3) was reduced to 5-10% of normal and the residual enzyme showed an apparent Kmorn of 0.69 (normal 0.37 +/- 0.10) mmol liter. The pH dependence was normal. The patient's mother also showed hyperammonemia but was not clinically affected. Consideration of the genetics of the disease suggested that many female patients should have a mixture of normal and mutant enzymes. Electrophoresis of the patient's liver extract showed an additional band of OTC activity probably due to this mutant enzyme. The ratio of plasma glutamate-pyruvate transaminase to OTC was abnormal in four clinically affected patients with OTC deficiency (Fig. 4B) but not in two of their mothers without clinical signs.", "PMID": 980551}
{"id": "pubmed23n0032_12439", "title": "Growth retardation and zinc nutrition.", "content": "Based on a previous report of a zinc deficiency syndrome in children characterized by low hair zinc, anorexia, poor growth, and hypogeusia, 12 children attending  a pediatric endocrinology clinic for growth retardation and judged as having short stature as a variant of normal (SVN) were evaluated as to their zinc nutriture to learn whether zinc deficiency was a contributory factor. None was found to have the above syndrome, although one child did have a hair zinc concentration below 70 mug/g. The mean hair zinc of the SVN subjects was lower than the hair zinc of 40 apparently normal adolescents, 131+/-37 mug/g vs. 168+/-44 mug/g (P less than 0.02), but there was no difference found in plasma levels of zinc or in taste acuity. In contrast, five patients with total growth arrest secondary to juvenile Crohn's disease (CD) were found to have multiple findings consistent with zinc deficiency including low plasma zinc, low hair zinc, or hypogeusia.", "contents": "Growth retardation and zinc nutrition. Based on a previous report of a zinc deficiency syndrome in children characterized by low hair zinc, anorexia, poor growth, and hypogeusia, 12 children attending  a pediatric endocrinology clinic for growth retardation and judged as having short stature as a variant of normal (SVN) were evaluated as to their zinc nutriture to learn whether zinc deficiency was a contributory factor. None was found to have the above syndrome, although one child did have a hair zinc concentration below 70 mug/g. The mean hair zinc of the SVN subjects was lower than the hair zinc of 40 apparently normal adolescents, 131+/-37 mug/g vs. 168+/-44 mug/g (P less than 0.02), but there was no difference found in plasma levels of zinc or in taste acuity. In contrast, five patients with total growth arrest secondary to juvenile Crohn's disease (CD) were found to have multiple findings consistent with zinc deficiency including low plasma zinc, low hair zinc, or hypogeusia.", "PMID": 980552}
{"id": "pubmed23n0032_12440", "title": "Growth and nutrition of uremic piglets.", "content": "Piglets aged 6 days were rendered uremic by subtotal nephrectomy and their growth and dietary intakes studied over the next 21 days. Eleven control piglets fed a voluntary intake of a sow's milk substitute (group A), 11 nephrectomized piglets fed a voluntary intake of the same feed (group B), 6 nephrectomized piglets tube fed the same milk (group C), and 11 nephrectomized piglets fed a voluntary intake of a low protein, isocaloric food (group D) were studied. After nephrectomy the piglets had an initial rapid rise in blood urea concentration which had fallen by day 7 and then leveled out around 13 mmol/liter in group B and 8 mmol/liter in group D. After operation control piglets (group A) ate more from day 4 and were larger from day 7 than the nephrectomized piglets (group B). Those piglets tube fed (group C) were of a similar size to the controls but all died between day 7 and day 11 with associated high blood urea concentrations. Piglets fed the low protein, isocaloric feed (group D) were smaller than both the controls and group B. They also ate less food than the controls and those nephrectomized piglets in group B which were on a voluntary intake of the normal feed.", "contents": "Growth and nutrition of uremic piglets. Piglets aged 6 days were rendered uremic by subtotal nephrectomy and their growth and dietary intakes studied over the next 21 days. Eleven control piglets fed a voluntary intake of a sow's milk substitute (group A), 11 nephrectomized piglets fed a voluntary intake of the same feed (group B), 6 nephrectomized piglets tube fed the same milk (group C), and 11 nephrectomized piglets fed a voluntary intake of a low protein, isocaloric food (group D) were studied. After nephrectomy the piglets had an initial rapid rise in blood urea concentration which had fallen by day 7 and then leveled out around 13 mmol/liter in group B and 8 mmol/liter in group D. After operation control piglets (group A) ate more from day 4 and were larger from day 7 than the nephrectomized piglets (group B). Those piglets tube fed (group C) were of a similar size to the controls but all died between day 7 and day 11 with associated high blood urea concentrations. Piglets fed the low protein, isocaloric feed (group D) were smaller than both the controls and group B. They also ate less food than the controls and those nephrectomized piglets in group B which were on a voluntary intake of the normal feed.", "PMID": 980553}
{"id": "pubmed23n0032_12441", "title": "Effect of undernutrition and amino acid deficiency on the timing of puberty in rats.", "content": "Sexual maturation was examined in underfed or amino acid-deficient rats. We have demonstrated a highly significant negative linear relationship (r = -0.80, P less than 0.001) between the age at puberty and the growth rate in rats under conditions of food restriction. The weight at puberty in animals growing at different rates because of undernutrition was not constant but behaved as a quadratic function of growth rate, as predicted from the assumption that growth rate was an independent variable. Growth rate is therefore more important than arrival at a particular fixed weight in determining the timing of puberty. Feeding of low valine diets resulted in delayed sexual maturation. Both the weight at vaginal opening (182 +/- 5.9 g) and the weight at first estrus (187 +/- 6.1 g) were significantly greater in the valine-deficient group when compared with growth-matched control (139 +/- 10.7 g and 161 +/- 9.3 g, respectively, P less than 0.05). The valine-deficient group also had significantly later vaginal opening (98.8+/- 4.7 days) than growth-matched controls (76.6 +/- 6.6 days, P less than 0.02). Valine deficiency seemed to have a specific effect on the hypothalamic-pituitary-gonadal axis since puberty in valine-deficient animals was delayed more than could be accounted for by impairment of growth.", "contents": "Effect of undernutrition and amino acid deficiency on the timing of puberty in rats. Sexual maturation was examined in underfed or amino acid-deficient rats. We have demonstrated a highly significant negative linear relationship (r = -0.80, P less than 0.001) between the age at puberty and the growth rate in rats under conditions of food restriction. The weight at puberty in animals growing at different rates because of undernutrition was not constant but behaved as a quadratic function of growth rate, as predicted from the assumption that growth rate was an independent variable. Growth rate is therefore more important than arrival at a particular fixed weight in determining the timing of puberty. Feeding of low valine diets resulted in delayed sexual maturation. Both the weight at vaginal opening (182 +/- 5.9 g) and the weight at first estrus (187 +/- 6.1 g) were significantly greater in the valine-deficient group when compared with growth-matched control (139 +/- 10.7 g and 161 +/- 9.3 g, respectively, P less than 0.05). The valine-deficient group also had significantly later vaginal opening (98.8+/- 4.7 days) than growth-matched controls (76.6 +/- 6.6 days, P less than 0.02). Valine deficiency seemed to have a specific effect on the hypothalamic-pituitary-gonadal axis since puberty in valine-deficient animals was delayed more than could be accounted for by impairment of growth.", "PMID": 980554}
{"id": "pubmed23n0032_12442", "title": "An evaluation of the safety of a low-saturated-fat, low-cholesterol diet beginning in infancy.", "content": "This study was designed to detect developmental, body chemistry, or somatic differences that might be present in children who had been on a low-saturated-fat, low-cholesterol (LSFLC) diet since birth. Comparison to a matched control group at age 3 years failed to demonstrate significant differences in percentile height, percentile weight, head circumference, age, skinfold thickness, total serum protein, hemoglobin, number of failures on a Denver Developmental Screening test, or number of sick visits. Experimental children did have significantly lower serum cholesterol than a large group of children of similar age. It is concluded that at age 3 years, no significant adverse findings were present in the group who had been nourished on a LSFLC diet since birth as compared to a control group.", "contents": "An evaluation of the safety of a low-saturated-fat, low-cholesterol diet beginning in infancy. This study was designed to detect developmental, body chemistry, or somatic differences that might be present in children who had been on a low-saturated-fat, low-cholesterol (LSFLC) diet since birth. Comparison to a matched control group at age 3 years failed to demonstrate significant differences in percentile height, percentile weight, head circumference, age, skinfold thickness, total serum protein, hemoglobin, number of failures on a Denver Developmental Screening test, or number of sick visits. Experimental children did have significantly lower serum cholesterol than a large group of children of similar age. It is concluded that at age 3 years, no significant adverse findings were present in the group who had been nourished on a LSFLC diet since birth as compared to a control group.", "PMID": 980598}
{"id": "pubmed23n0032_12443", "title": "Relationship of passive cigarette-smoking to sudden infant death syndrome.", "content": "The smoking habits of 56 families who lost babies to the sudden infant death syndrome (SIDS) were compared to those of 86 control families. A higher proportion of SIDS mothers smoked both during pregnancy (61% vs. 42%) and after their babies were born (59% vs. 37%). SIDS mother also smoked a significantly greater number of cigarettes than controls. Exposure to cigarette smoke (\"passive smoking\") appears to enhance the risk of SIDS for reasons not known.", "contents": "Relationship of passive cigarette-smoking to sudden infant death syndrome. The smoking habits of 56 families who lost babies to the sudden infant death syndrome (SIDS) were compared to those of 86 control families. A higher proportion of SIDS mothers smoked both during pregnancy (61% vs. 42%) and after their babies were born (59% vs. 37%). SIDS mother also smoked a significantly greater number of cigarettes than controls. Exposure to cigarette smoke (\"passive smoking\") appears to enhance the risk of SIDS for reasons not known.", "PMID": 980599}
{"id": "pubmed23n0032_12444", "title": "Respiratory distress syndrome of the newborn and complications of pregnancy.", "content": "A group of 412 infants with birthweights between 501 and 2,500 gm and gestational ages of 36 weeks or less were studied for the influence of both prolonged ruputre of amniotic membranes and maternal hypertension on the incidence of idiopathic respiratory distress syndrome (IRDS). The occurrence of these complications was associated with a significant decrease in the incidence of IRDS only in infants with birthweights between 1,501 and 2,500 gm (37.4% in the no complications group versus 12.8% in the complications group; P less than .01) or gestational ages of 33 to 36 weeks (35.2% in the no complications group versus 13.1% in the complications group; P less than .01). In infants with birthweights of 1,500 gm or less or gestational ages of 32 weeks or less, the specific antecedent complications of pregnancy did not alter the incidence of IRDS. Rupture of the membranes for more than 72 hours had no greater effect on the incidence of IRDS than those lasting 24 to 72 hours.", "contents": "Respiratory distress syndrome of the newborn and complications of pregnancy. A group of 412 infants with birthweights between 501 and 2,500 gm and gestational ages of 36 weeks or less were studied for the influence of both prolonged ruputre of amniotic membranes and maternal hypertension on the incidence of idiopathic respiratory distress syndrome (IRDS). The occurrence of these complications was associated with a significant decrease in the incidence of IRDS only in infants with birthweights between 1,501 and 2,500 gm (37.4% in the no complications group versus 12.8% in the complications group; P less than .01) or gestational ages of 33 to 36 weeks (35.2% in the no complications group versus 13.1% in the complications group; P less than .01). In infants with birthweights of 1,500 gm or less or gestational ages of 32 weeks or less, the specific antecedent complications of pregnancy did not alter the incidence of IRDS. Rupture of the membranes for more than 72 hours had no greater effect on the incidence of IRDS than those lasting 24 to 72 hours.", "PMID": 980600}
{"id": "pubmed23n0032_12445", "title": "Differential effects of maternal heroin and methadone use on birthweight.", "content": "An analysis of birthweights of 337 neonates in relation to history of maternal narcotic usage was undertaken. Mean birthweight of infants born to mothers abusing heroin during the pregnancy was 2,490 gm, an effect primarily of intrauterine growth retardation. Low mean birthweight (2,615 gm) was also seen in infants born to mothers who had abused heroin only prior to this pregnancy, and mothers who had used both heroin and methadone during the pregnancy (2,535 gm). Infants born to mothers on methadone maintenance during the pregnancy had significantly higher mean birthweights (2.961 gm), but lower than the control group (3,176 gm). A highly significant relationship was observed between maternal methadone dosage in the first trimester and birthweight, i.e., the higher the dosage, the larger the infant. Heroin causes fetal growth retardation, an effect which may persist beyond the period of addiction. Methadone may promote fetal growth in a dose-related fashion after maternal use of heroin.", "contents": "Differential effects of maternal heroin and methadone use on birthweight. An analysis of birthweights of 337 neonates in relation to history of maternal narcotic usage was undertaken. Mean birthweight of infants born to mothers abusing heroin during the pregnancy was 2,490 gm, an effect primarily of intrauterine growth retardation. Low mean birthweight (2,615 gm) was also seen in infants born to mothers who had abused heroin only prior to this pregnancy, and mothers who had used both heroin and methadone during the pregnancy (2,535 gm). Infants born to mothers on methadone maintenance during the pregnancy had significantly higher mean birthweights (2.961 gm), but lower than the control group (3,176 gm). A highly significant relationship was observed between maternal methadone dosage in the first trimester and birthweight, i.e., the higher the dosage, the larger the infant. Heroin causes fetal growth retardation, an effect which may persist beyond the period of addiction. Methadone may promote fetal growth in a dose-related fashion after maternal use of heroin.", "PMID": 980601}
{"id": "pubmed23n0032_12446", "title": "Municipal child health services: a ten-year reorganization.", "content": "There has been increasing interest within the medical community in developing primary care programs in teaching hospitals that meet the medical needs of a community. The Cambridge Hospital system provides a working model for such a mutually beneficial interaction. Based on principles of administrative centralization and utilization of the pediatric nurse practitioner, the neighborhood health centers operate as part of a cost-effective series of concentric circles. Administrative centralization within the Department of Pediatrics at the Cambridge Hospital has permitted the elimination of duplicate medical services and the development of a responsive community health service. The pediatric nurse practitioner under close senior staff supervision, serves as the primary caretaker in the neighborhood centers. In addition to her role as the provider of a protocol-determined, age-related series of physical psychometric, and laboratory examinations the pediatric nurse practitioner also cares for minor injuries and illnesses of childhood. Any child deviating from a clearly established range of norms for any physical, developmental, or laboratory parameter is immediately referred to the senior staff pediatrician for further evaluation. Finally, any patient requiring subspecialized inpatient care is referred to the Massachusetts General Hospital. The senior pediatric staff of the Cambridge Hospital serves the function of coordinating and supporting the rapid interlevel communication which must occur between community service, primary hospital, and a tertiary care facility. This neighborhood health care system is, we feel, a medically sound, educationally exciting, and fiscally responsible solution to a problem facing many urban areas and their academic medical institutions.", "contents": "Municipal child health services: a ten-year reorganization. There has been increasing interest within the medical community in developing primary care programs in teaching hospitals that meet the medical needs of a community. The Cambridge Hospital system provides a working model for such a mutually beneficial interaction. Based on principles of administrative centralization and utilization of the pediatric nurse practitioner, the neighborhood health centers operate as part of a cost-effective series of concentric circles. Administrative centralization within the Department of Pediatrics at the Cambridge Hospital has permitted the elimination of duplicate medical services and the development of a responsive community health service. The pediatric nurse practitioner under close senior staff supervision, serves as the primary caretaker in the neighborhood centers. In addition to her role as the provider of a protocol-determined, age-related series of physical psychometric, and laboratory examinations the pediatric nurse practitioner also cares for minor injuries and illnesses of childhood. Any child deviating from a clearly established range of norms for any physical, developmental, or laboratory parameter is immediately referred to the senior staff pediatrician for further evaluation. Finally, any patient requiring subspecialized inpatient care is referred to the Massachusetts General Hospital. The senior pediatric staff of the Cambridge Hospital serves the function of coordinating and supporting the rapid interlevel communication which must occur between community service, primary hospital, and a tertiary care facility. This neighborhood health care system is, we feel, a medically sound, educationally exciting, and fiscally responsible solution to a problem facing many urban areas and their academic medical institutions.", "PMID": 980603}
{"id": "pubmed23n0032_12447", "title": "Familial aggregation of blood pressures of newborn infants and their mother.", "content": "The blood pressures and pulse rates of 257 normal full-term infants and their mothers were measured two to four days after birth. Birthweight was correlated with systolic (P=.038), but not with diastolic blood pressure. Infants who were asleep had significantly lower systolic and diastolic blood pressure than infants who were awake (P less than .001). Sex, body length, and feedings did not appear to influence infant's blood pressure nor did the anesthesia given to the mothers. Maternal diastolic pressure correlated with infant's diastolic pressure (regression coefficient, .128) (P less than .01), whereas for systolic pressure the regression coefficient between maternal and infant pressure was .085 (P=NS). The aggregation between the diastolic blood pressures of infants and mothers was not influenced by birthweight, age of the mother, or medication administered to the mother. The pulse rates of black infants were significantly higher than those of white infants (P less than .002). There was no correlation between the pulse rates and blood pressures in infants.", "contents": "Familial aggregation of blood pressures of newborn infants and their mother. The blood pressures and pulse rates of 257 normal full-term infants and their mothers were measured two to four days after birth. Birthweight was correlated with systolic (P=.038), but not with diastolic blood pressure. Infants who were asleep had significantly lower systolic and diastolic blood pressure than infants who were awake (P less than .001). Sex, body length, and feedings did not appear to influence infant's blood pressure nor did the anesthesia given to the mothers. Maternal diastolic pressure correlated with infant's diastolic pressure (regression coefficient, .128) (P less than .01), whereas for systolic pressure the regression coefficient between maternal and infant pressure was .085 (P=NS). The aggregation between the diastolic blood pressures of infants and mothers was not influenced by birthweight, age of the mother, or medication administered to the mother. The pulse rates of black infants were significantly higher than those of white infants (P less than .002). There was no correlation between the pulse rates and blood pressures in infants.", "PMID": 980604}
{"id": "pubmed23n0032_12448", "title": "Serum enzyme abnormalities in juvenile rheumatoid arthritis.", "content": "Elevated serum transaminases, particularly SGOT, as a result of acetylsalicylic acid (ASA) therapy have been reported in patients with juvenile rheumatoid arthritis (JRA). In order to evaluate the possibilities that these elevated transaminases may result from JRA itself or from concomitant muscle injury, we correlated liver function tests and a specific test for muscle damage, creatine phosphokinase (CPK), with ASA therapy in 37 patients. These JRA patients were evaluated serially; 20 took ASA continuously, 6 took it intermittently, and 11 were on no therapy. Thirty-five healthy children were also studied to establish normal control values for the serum enzyme tests. Mean SGOT and SGPT in the 11 untreated subjects were significantly (P less than.001) higher than normal controls while CPK and alkaline phosphatase (AP) were not elevated. Mean SGOT and SGPT were also significantly (P less than .001) elevated in 20 children receiving ASA continuously; CPK was normal and AP less (P less than .05) than normal. CPK was elevated in 13 patients. Elevation of enzymes was sporadic and there was no correlation with serum salicylate, sex, age, disease duration, type, or activity. We conclude that mild abnormalities of SGOT and SGPT in JRA patients are common, but that they occur sporadically and elevated values appear to be unrelated to ASA therapy.", "contents": "Serum enzyme abnormalities in juvenile rheumatoid arthritis. Elevated serum transaminases, particularly SGOT, as a result of acetylsalicylic acid (ASA) therapy have been reported in patients with juvenile rheumatoid arthritis (JRA). In order to evaluate the possibilities that these elevated transaminases may result from JRA itself or from concomitant muscle injury, we correlated liver function tests and a specific test for muscle damage, creatine phosphokinase (CPK), with ASA therapy in 37 patients. These JRA patients were evaluated serially; 20 took ASA continuously, 6 took it intermittently, and 11 were on no therapy. Thirty-five healthy children were also studied to establish normal control values for the serum enzyme tests. Mean SGOT and SGPT in the 11 untreated subjects were significantly (P less than.001) higher than normal controls while CPK and alkaline phosphatase (AP) were not elevated. Mean SGOT and SGPT were also significantly (P less than .001) elevated in 20 children receiving ASA continuously; CPK was normal and AP less (P less than .05) than normal. CPK was elevated in 13 patients. Elevation of enzymes was sporadic and there was no correlation with serum salicylate, sex, age, disease duration, type, or activity. We conclude that mild abnormalities of SGOT and SGPT in JRA patients are common, but that they occur sporadically and elevated values appear to be unrelated to ASA therapy.", "PMID": 980605}
{"id": "pubmed23n0032_12449", "title": "A useful radiologic sign for the diagnosis of Turner's syndrome.", "content": "A new X-ray sign of gonadal dysgenesis is described. In this series nine out of ten patients with Turner's syndrome have a coarse, reticular pattern of the carpal bones. A comparison with other previously described roentgenographic characteristics on hand and wrist films of these children leads to the conclusion that the new sign is more reliable and specific.", "contents": "A useful radiologic sign for the diagnosis of Turner's syndrome. A new X-ray sign of gonadal dysgenesis is described. In this series nine out of ten patients with Turner's syndrome have a coarse, reticular pattern of the carpal bones. A comparison with other previously described roentgenographic characteristics on hand and wrist films of these children leads to the conclusion that the new sign is more reliable and specific.", "PMID": 980606}
{"id": "pubmed23n0032_12450", "title": "Developmental changes in the infants attention to pattern detail.", "content": "To assess developmental changes in the importance of stimulus intensity and pattern detail for infants' visual attention, infants of 10, 17, and 25 wk. of age were shown pairs of stimuli equated for stimulus intensity but different in pattern detail (angles or straight lines). The youngest infants gave equivalent amounts of attention to the stimuli of each pair while the two older groups showed a significant preference for the angular stimuli. The results are interpreted as showing the increased attention to elements with development, and a possible explanation of the preference for the angle stimuli is discussed.", "contents": "Developmental changes in the infants attention to pattern detail. To assess developmental changes in the importance of stimulus intensity and pattern detail for infants' visual attention, infants of 10, 17, and 25 wk. of age were shown pairs of stimuli equated for stimulus intensity but different in pattern detail (angles or straight lines). The youngest infants gave equivalent amounts of attention to the stimuli of each pair while the two older groups showed a significant preference for the angular stimuli. The results are interpreted as showing the increased attention to elements with development, and a possible explanation of the preference for the angle stimuli is discussed.", "PMID": 980629}
{"id": "pubmed23n0032_12451", "title": "Feelings of inexperienced therapists in psychotherapy: a factor analytic study.", "content": "The Therapy Session Report, a parallel-structured response questionnaire, was utilized to study the feelings of inexperienced therapists. 57 college student applicants at a university counseling center reported on their perceptions of their therapists' feelings immediately following an intake interview. The 19 therapists, clinical psychology graduate students in training, also reported what they felt during the intake session. Factor analysis of patients, perceptions of their therapists' feeling resulted in four factors, the first two accounting for 85% of the common variance. Factor analysis of therapists, perceptions of their own feelings resulted in nine factors. Results suggest that, while patients view their therapists in generally negative positive terms, inexperienced therapists experience considerable affective discomfort during their intake sessions.", "contents": "Feelings of inexperienced therapists in psychotherapy: a factor analytic study. The Therapy Session Report, a parallel-structured response questionnaire, was utilized to study the feelings of inexperienced therapists. 57 college student applicants at a university counseling center reported on their perceptions of their therapists' feelings immediately following an intake interview. The 19 therapists, clinical psychology graduate students in training, also reported what they felt during the intake session. Factor analysis of patients, perceptions of their therapists' feeling resulted in four factors, the first two accounting for 85% of the common variance. Factor analysis of therapists, perceptions of their own feelings resulted in nine factors. Results suggest that, while patients view their therapists in generally negative positive terms, inexperienced therapists experience considerable affective discomfort during their intake sessions.", "PMID": 980630}
{"id": "pubmed23n0032_12452", "title": "Relation between optically induced and tracking-limb-movement curvature in a visual capture phenomenon.", "content": "Two experiments were conducted to help determine the nature of visual capture in the perception of curvature. If an individual finger-tracks a straight edge while viewing limb movements through a curve-inducing lens, the edge is reported to feel curved even though the finger is moving in a straight path. It has recently been demonstrated, however, that the finger actually tracks the straight edge through a curved path with the same orientation as the induced visual curve. In order to specify more completely the degree of relation between the visual and tracking (motor) curves the first experiment determined the fate of tracking limb-movement curvature when the eyes were closed. The second experiment determined whether curvature of tracking limb movement decreased as the magnitude of the visual curve was decreased. Results from both experiments supported the contention that curved tracking limb movement may mediate the visual and felt curves. The nature of the mechanism underlying felt curvature in visual capture was discussed.", "contents": "Relation between optically induced and tracking-limb-movement curvature in a visual capture phenomenon. Two experiments were conducted to help determine the nature of visual capture in the perception of curvature. If an individual finger-tracks a straight edge while viewing limb movements through a curve-inducing lens, the edge is reported to feel curved even though the finger is moving in a straight path. It has recently been demonstrated, however, that the finger actually tracks the straight edge through a curved path with the same orientation as the induced visual curve. In order to specify more completely the degree of relation between the visual and tracking (motor) curves the first experiment determined the fate of tracking limb-movement curvature when the eyes were closed. The second experiment determined whether curvature of tracking limb movement decreased as the magnitude of the visual curve was decreased. Results from both experiments supported the contention that curved tracking limb movement may mediate the visual and felt curves. The nature of the mechanism underlying felt curvature in visual capture was discussed.", "PMID": 980631}
{"id": "pubmed23n0032_12453", "title": "Selective hyperthermia and reaction time.", "content": "Two reaction time studies are reported in which subjects responded under conditions of selective hyperthermia. Wearing a helmet prototype, the cortical surface of the subject was thermally stressed to a level 1.11 degrees C over baseline as measured by a thermocouple placed against the tympanic membrane. In a simple choice RT task, both reaction time and response variability were decreased compared to performance in either a control or placebo condition. Latency in Neisser's visual scanning task was also shortened under conditions of this thermal stress localized in the cortical region. In both cases, there was no observed decrement in accuracy or level of performance. Enhanced human performance resulting from the maintenance of cortical temperature above the normal diurnal range is implied.", "contents": "Selective hyperthermia and reaction time. Two reaction time studies are reported in which subjects responded under conditions of selective hyperthermia. Wearing a helmet prototype, the cortical surface of the subject was thermally stressed to a level 1.11 degrees C over baseline as measured by a thermocouple placed against the tympanic membrane. In a simple choice RT task, both reaction time and response variability were decreased compared to performance in either a control or placebo condition. Latency in Neisser's visual scanning task was also shortened under conditions of this thermal stress localized in the cortical region. In both cases, there was no observed decrement in accuracy or level of performance. Enhanced human performance resulting from the maintenance of cortical temperature above the normal diurnal range is implied.", "PMID": 980632}
{"id": "pubmed23n0032_12454", "title": "Institutional males' perception of three counseling techniques.", "content": "The use of advice-giving, Adlerian interpretation, and analytically derived interpretation with regard to whether feelings of approach, attack, or withdrawal were elicited was investigated by having subjects view a videotape of eight-role-played counseling segments. The videotape presented problem-statements by a role-playing adolescent followed by a role-playing counselor who made three separate responses to the statements. Subjects were 20 internally and 20 externally controlled incarcerated youth who indicated how each counselor's response made them feel (approach, attack, or withdrawal). Both internally and externally controlled subjects tended to respond to Adlerian interpretation and analytically derived interpretation with attack - and withdrawal - feelings and to advice-giving with approach-feelings in this quasi-counseling situation.", "contents": "Institutional males' perception of three counseling techniques. The use of advice-giving, Adlerian interpretation, and analytically derived interpretation with regard to whether feelings of approach, attack, or withdrawal were elicited was investigated by having subjects view a videotape of eight-role-played counseling segments. The videotape presented problem-statements by a role-playing adolescent followed by a role-playing counselor who made three separate responses to the statements. Subjects were 20 internally and 20 externally controlled incarcerated youth who indicated how each counselor's response made them feel (approach, attack, or withdrawal). Both internally and externally controlled subjects tended to respond to Adlerian interpretation and analytically derived interpretation with attack - and withdrawal - feelings and to advice-giving with approach-feelings in this quasi-counseling situation.", "PMID": 980633}
{"id": "pubmed23n0032_12455", "title": "Usefulness of the group-comparison method to demonstrate sex differences in spatial orientation and spatial visualization in older men and women.", "content": "This paper reports an analysis of sex differences in cognitive test scores covering the dimensions of spatial orientation and spatial visualization in groups of 6 older men and 6 women matched for speed of performance on a maze test and level of performance on a spatial relations task. Older men were more proficient solving spatial problems using the body as a referent, whereas there was no significant difference between the sexes in imagining spatial displacement. Matched comparisons appear a useful adjunct to population research to understand the type(s) of cognitive processes where differential performance by the sexes is observed.", "contents": "Usefulness of the group-comparison method to demonstrate sex differences in spatial orientation and spatial visualization in older men and women. This paper reports an analysis of sex differences in cognitive test scores covering the dimensions of spatial orientation and spatial visualization in groups of 6 older men and 6 women matched for speed of performance on a maze test and level of performance on a spatial relations task. Older men were more proficient solving spatial problems using the body as a referent, whereas there was no significant difference between the sexes in imagining spatial displacement. Matched comparisons appear a useful adjunct to population research to understand the type(s) of cognitive processes where differential performance by the sexes is observed.", "PMID": 980634}
{"id": "pubmed23n0032_12456", "title": "Assessment of visual field impairment by objective technique.", "content": "An objective psychophysical technique for investigating visual fields by averaged scalp potentials evoked by pattern gratings of alternating contrast and by sinusoidally modulated flicerking light is applied to a child with a right homonomous hemianopsia. This technique illustrates a value, in obtaining clinical data on visual fields in situations were subjective perimetry is difficult to administer or where conservating diagnostic methods do not yield evidence of pathology.", "contents": "Assessment of visual field impairment by objective technique. An objective psychophysical technique for investigating visual fields by averaged scalp potentials evoked by pattern gratings of alternating contrast and by sinusoidally modulated flicerking light is applied to a child with a right homonomous hemianopsia. This technique illustrates a value, in obtaining clinical data on visual fields in situations were subjective perimetry is difficult to administer or where conservating diagnostic methods do not yield evidence of pathology.", "PMID": 980635}
{"id": "pubmed23n0032_12457", "title": "Field dependence among male and female alcoholics: II. Norms for the rod-and-frame test.", "content": "402 males and 160 females hospitalized for treatment of alcoholism were tested in a standardized manner on the Rod-and-frame test as a means of supplementing an earlier report of normative data on perceptual style among male alcoholics. When their performance was contrasted with that of normal and psychiatric samples, alcoholics were clearly the most field dependent of all groups studied. Statistically significant sex differences justify the need for separate norms for males and females.", "contents": "Field dependence among male and female alcoholics: II. Norms for the rod-and-frame test. 402 males and 160 females hospitalized for treatment of alcoholism were tested in a standardized manner on the Rod-and-frame test as a means of supplementing an earlier report of normative data on perceptual style among male alcoholics. When their performance was contrasted with that of normal and psychiatric samples, alcoholics were clearly the most field dependent of all groups studied. Statistically significant sex differences justify the need for separate norms for males and females.", "PMID": 980637}
{"id": "pubmed23n0032_12458", "title": "On the validity of hypotheses derived from the Rorschach: 1. The relationship between color and affect.", "content": "Interpretation of response to color on the Rorschach rests on Rorschach's assertion that color has affective connotations. His assumption was based on the connection of color and affect in culture and as regards the differential response to color by patients he tested. This review focused on research which could lend support to Rorschach's empirically derived notion. Ignoring clinical studies of psychiatric patients (in the absence of any theory of affects unique to specific psychiatric groups), this review focuses primarily on psychological tests of the hypothesis. Affect does seem to be associated with colors but not so specifically as to be useful in interpreting the meaning of the response by any one person. Moreover, affect was seen to affect the many dimensions of the Rorschach stimuli. Interpretation of the response to color must take into account the unsystematic way in which factors influence response to color on the Rorschach, e.g. saturation, context, area covered, etc, vary from card to card. These factors alone influence the response to color and make it impossible to draw conclusions using the plates as constructed. The interpretation of affect from response to color on the Rorschach was seriously questioned.", "contents": "On the validity of hypotheses derived from the Rorschach: 1. The relationship between color and affect. Interpretation of response to color on the Rorschach rests on Rorschach's assertion that color has affective connotations. His assumption was based on the connection of color and affect in culture and as regards the differential response to color by patients he tested. This review focused on research which could lend support to Rorschach's empirically derived notion. Ignoring clinical studies of psychiatric patients (in the absence of any theory of affects unique to specific psychiatric groups), this review focuses primarily on psychological tests of the hypothesis. Affect does seem to be associated with colors but not so specifically as to be useful in interpreting the meaning of the response by any one person. Moreover, affect was seen to affect the many dimensions of the Rorschach stimuli. Interpretation of the response to color must take into account the unsystematic way in which factors influence response to color on the Rorschach, e.g. saturation, context, area covered, etc, vary from card to card. These factors alone influence the response to color and make it impossible to draw conclusions using the plates as constructed. The interpretation of affect from response to color on the Rorschach was seriously questioned.", "PMID": 980638}
{"id": "pubmed23n0032_12459", "title": "Visual orientational anisotropy and stimulus surround effects in discrimination of spatial numerosity.", "content": "Visual spatial numerosity perception was investigated as a function of spatial orientation and surround conditions. 3 to 13 dots arranged linearly in horizontal, vertical, or oblique orientations were presented for 200 msec. in either a square or circular stimulus field. For patterns with small numbers of dots (3 to 7), surround and orientation conditions did not substantially alter perceived number except vertical arrangements were judged as having reliably more dots than identical horizontal arrangements. For large dot-number patterns (8 to 13), surround significantly altered perceived number and left oblique linear arrangements were judged to have reliably more dots than equivalent arrangements in all other orientations.", "contents": "Visual orientational anisotropy and stimulus surround effects in discrimination of spatial numerosity. Visual spatial numerosity perception was investigated as a function of spatial orientation and surround conditions. 3 to 13 dots arranged linearly in horizontal, vertical, or oblique orientations were presented for 200 msec. in either a square or circular stimulus field. For patterns with small numbers of dots (3 to 7), surround and orientation conditions did not substantially alter perceived number except vertical arrangements were judged as having reliably more dots than identical horizontal arrangements. For large dot-number patterns (8 to 13), surround significantly altered perceived number and left oblique linear arrangements were judged to have reliably more dots than equivalent arrangements in all other orientations.", "PMID": 980639}
{"id": "pubmed23n0032_12460", "title": "Prolonged practice of a simple motor task by preferred and nonpreffered hands.", "content": "After prolonged practice (1300 10 sec. trials) the tapping speed of the nonpreferred hand reached that of the preferred hand on a simple finger-tapping task. Analysis of the intertrap intervals showed the variability of the duration of intertrap intervals was smaller for the preferred than for the nonpreferred hand, the difference was not affected by the prolonged practice for N = 1.", "contents": "Prolonged practice of a simple motor task by preferred and nonpreffered hands. After prolonged practice (1300 10 sec. trials) the tapping speed of the nonpreferred hand reached that of the preferred hand on a simple finger-tapping task. Analysis of the intertrap intervals showed the variability of the duration of intertrap intervals was smaller for the preferred than for the nonpreferred hand, the difference was not affected by the prolonged practice for N = 1.", "PMID": 980640}
{"id": "pubmed23n0032_12461", "title": "Subjective feelings and vascular responses in reaction to reduced control over aversiveness.", "content": "Subjects were exposed to an aversive, modified Sidman avoidance procedure. One group had relative control over the aversiveness in that they could exercise an option for unlimited rest periods, and they could avoid electric shock by an appropriately fast-response rate on a monitoring task. The second group was yoked to subjects in Group I both with respect to rests and shocks, thereby producing decreased capability of behavioral control. The effects of this manipulation were evaluated by three types of dependent measures: vascular arousal (systolic and diastolic blood pressure), verbal reports of subjective feelings, and performance on a subsequent problem-solving task. The findings suggested motivational deficits along with reduced vascular arousal among the yoked subjects, a pattern of results which may clarify some of the alternative hypotheses pertinent to \"learned helplessness\".", "contents": "Subjective feelings and vascular responses in reaction to reduced control over aversiveness. Subjects were exposed to an aversive, modified Sidman avoidance procedure. One group had relative control over the aversiveness in that they could exercise an option for unlimited rest periods, and they could avoid electric shock by an appropriately fast-response rate on a monitoring task. The second group was yoked to subjects in Group I both with respect to rests and shocks, thereby producing decreased capability of behavioral control. The effects of this manipulation were evaluated by three types of dependent measures: vascular arousal (systolic and diastolic blood pressure), verbal reports of subjective feelings, and performance on a subsequent problem-solving task. The findings suggested motivational deficits along with reduced vascular arousal among the yoked subjects, a pattern of results which may clarify some of the alternative hypotheses pertinent to \"learned helplessness\".", "PMID": 980641}
{"id": "pubmed23n0032_12462", "title": "Handedness in relation to measures of motor and tactile-perceptual functions in normal children.", "content": "10 boys and 10 girls were tested at each of six age levels (6, 7, 8, 12, 13, and 14 yr). All of the children were right-handed, and at each age level the groups did not differ in age, WISC Full Scale IQ, or educational attainment. The groups were compared on motor tasks (strength and speed) and tactile-perceptual tasks (finger localization and symbol recognition). The performances of the right and left hands were compared. The results indicated clear right-hand motor superiority, but no \"sidedness\" effect for the tactile-perceptual measures. The implication of these results for brain-behaviour relationships was discussed.", "contents": "Handedness in relation to measures of motor and tactile-perceptual functions in normal children. 10 boys and 10 girls were tested at each of six age levels (6, 7, 8, 12, 13, and 14 yr). All of the children were right-handed, and at each age level the groups did not differ in age, WISC Full Scale IQ, or educational attainment. The groups were compared on motor tasks (strength and speed) and tactile-perceptual tasks (finger localization and symbol recognition). The performances of the right and left hands were compared. The results indicated clear right-hand motor superiority, but no \"sidedness\" effect for the tactile-perceptual measures. The implication of these results for brain-behaviour relationships was discussed.", "PMID": 980642}
{"id": "pubmed23n0032_12463", "title": "Experimental control of rhythm in stereotyped body rocking.", "content": "A technique is described which enabled external control of the rhythm of stereotyped body rocking in a profoundly retarded, non-verbal subject. This technique consists of training the individual to synchronize the rhythm of his body rocking with an externally driven pacemaker light. Evidence is presented for experimental control of rhythm in a profoundly retarded, institutionalized child with a chronic history of body rocking.", "contents": "Experimental control of rhythm in stereotyped body rocking. A technique is described which enabled external control of the rhythm of stereotyped body rocking in a profoundly retarded, non-verbal subject. This technique consists of training the individual to synchronize the rhythm of his body rocking with an externally driven pacemaker light. Evidence is presented for experimental control of rhythm in a profoundly retarded, institutionalized child with a chronic history of body rocking.", "PMID": 980643}
{"id": "pubmed23n0032_12464", "title": "Effect of degree of separation of visual-auditory stimulus and eye position upon spatial interaction of vision and audition.", "content": "Subjects were exposed to a visual and to an auditory stimulus that differed spatially in laterality of origin. The subjects were observed for visual biasing of auditory localization (the momentary influence of a light on the spatially perceived location of a simultaneously presented sound) and for auditory aftereffect (a change in perceived location of a sound that persists over time and is measured after termination of the visual stimulus). A significant effect of visual stimulation on auditory localization was found only with the measure of bias. Bias was tested as a function of degree of visual-auditory separation (10/20/30 degrees), eye position (straight-ahead/visual stimulus fixation), and position of visual stimulus relative to auditory stimulus (left/right). Only eye position proved statistically significant; straight-ahead eye position induced more bias than did fixation of the visual stimulus.", "contents": "Effect of degree of separation of visual-auditory stimulus and eye position upon spatial interaction of vision and audition. Subjects were exposed to a visual and to an auditory stimulus that differed spatially in laterality of origin. The subjects were observed for visual biasing of auditory localization (the momentary influence of a light on the spatially perceived location of a simultaneously presented sound) and for auditory aftereffect (a change in perceived location of a sound that persists over time and is measured after termination of the visual stimulus). A significant effect of visual stimulation on auditory localization was found only with the measure of bias. Bias was tested as a function of degree of visual-auditory separation (10/20/30 degrees), eye position (straight-ahead/visual stimulus fixation), and position of visual stimulus relative to auditory stimulus (left/right). Only eye position proved statistically significant; straight-ahead eye position induced more bias than did fixation of the visual stimulus.", "PMID": 980644}
{"id": "pubmed23n0032_12465", "title": "Clothing as a nonverbal communicator or sexual attitudes and behavior.", "content": "To test the hypotheses that people believe that certain kinds and styles of clothing are indicative of liberal sexual attitudes and behavior and these beliefs are correct, questionnaires were filled out by 259 subjects. These questionnaires asked each subject for the frequencies with which he wore various items and styles of clothing, his attitude toward premarital sexual relations, the number of persons with whom he had had sexual relations, the frequencies with which he believed sexually liberal men and women wore various items and styles of clothing, and the frequencies with which he felt sexually conservative men and women wore various items and styles of clothing. Correlations showed that, although subjects believed that a large number of items and styles of clothing are indicative of liberal sexual attitudes and behavior, only a few items and styles actually were associated with liberal sexual attitudes and reported behavior.", "contents": "Clothing as a nonverbal communicator or sexual attitudes and behavior. To test the hypotheses that people believe that certain kinds and styles of clothing are indicative of liberal sexual attitudes and behavior and these beliefs are correct, questionnaires were filled out by 259 subjects. These questionnaires asked each subject for the frequencies with which he wore various items and styles of clothing, his attitude toward premarital sexual relations, the number of persons with whom he had had sexual relations, the frequencies with which he believed sexually liberal men and women wore various items and styles of clothing, and the frequencies with which he felt sexually conservative men and women wore various items and styles of clothing. Correlations showed that, although subjects believed that a large number of items and styles of clothing are indicative of liberal sexual attitudes and behavior, only a few items and styles actually were associated with liberal sexual attitudes and reported behavior.", "PMID": 980646}
{"id": "pubmed23n0032_12466", "title": "Distribution of movement time in a target-aiming task.", "content": "Various characteristics of the distribution of movement time were analyzed for a task involving serial and rotary arm movements aimed at a target. For experimental data generated from five Ss, (a) the distribution tended to be unimodal and more peaked than a normal distribution, (b) the skewness of the distribution was predominantly positive and (c) the standard deviation, in addition to the mean, of movement time was significantly affected by the complexity of the task as measured by Fitts' index of difficulty, while the skewness and the kurtosis were not. For the average results for the Ss, a first-order linear model with the standard deviation of movement time as the dependent variable and Fitts' index as the independent one explained 67% of the variation in standard deviation as compared to 98% of the variation in mean movement time accounted for by the Fitts' index.", "contents": "Distribution of movement time in a target-aiming task. Various characteristics of the distribution of movement time were analyzed for a task involving serial and rotary arm movements aimed at a target. For experimental data generated from five Ss, (a) the distribution tended to be unimodal and more peaked than a normal distribution, (b) the skewness of the distribution was predominantly positive and (c) the standard deviation, in addition to the mean, of movement time was significantly affected by the complexity of the task as measured by Fitts' index of difficulty, while the skewness and the kurtosis were not. For the average results for the Ss, a first-order linear model with the standard deviation of movement time as the dependent variable and Fitts' index as the independent one explained 67% of the variation in standard deviation as compared to 98% of the variation in mean movement time accounted for by the Fitts' index.", "PMID": 980647}
{"id": "pubmed23n0032_12467", "title": "Anxiety, task-experience, and vocal-style of judges as determinants of performer confidence.", "content": "To examine the effect of umpiring vocal-manner on a performance-measure of confidence, 30 baseball players viewed a series of videotaped pitching-catching-umpiring sequences and rated confidence in their ability to hit each pitch. Matched sequences were presented under each of two controlled conditions: for Conventional, the umpire called \"strikes\" emphatically and \"balls\" in an indifferent manner; for Modified, this vocal-manner was reversed. A single Conventional (or, Modified) sequence embedded within a series of contrasting sequences and a \"bad\" pitch (clearly out of the strike-zone) were included as tests of the effectiveness of the experimental manipulation. Results indicated: (1) Modified umpiring enhanced confidence; (2) this effect was most prominent among highly experienced players; (3) low-anxiety players showed higher confidence under both conditions; (4) Modified sequences embedded within a Conventional background elicited significantly greater confidence than in the background sequences.", "contents": "Anxiety, task-experience, and vocal-style of judges as determinants of performer confidence. To examine the effect of umpiring vocal-manner on a performance-measure of confidence, 30 baseball players viewed a series of videotaped pitching-catching-umpiring sequences and rated confidence in their ability to hit each pitch. Matched sequences were presented under each of two controlled conditions: for Conventional, the umpire called \"strikes\" emphatically and \"balls\" in an indifferent manner; for Modified, this vocal-manner was reversed. A single Conventional (or, Modified) sequence embedded within a series of contrasting sequences and a \"bad\" pitch (clearly out of the strike-zone) were included as tests of the effectiveness of the experimental manipulation. Results indicated: (1) Modified umpiring enhanced confidence; (2) this effect was most prominent among highly experienced players; (3) low-anxiety players showed higher confidence under both conditions; (4) Modified sequences embedded within a Conventional background elicited significantly greater confidence than in the background sequences.", "PMID": 980648}
{"id": "pubmed23n0032_12468", "title": "Attitude and personality differences in physically active and inactive university teachers.", "content": "This study assessed peronality factors and attitudes toward physical activity in a sample of university teachers. One group (N = 20) of subjects regularly participated in various forms of physical activity; the second (N = 20) showed no such interest. The psychological measures used were the Cattell 16 PF Form A and the Kenyon questionnaire on attitudes toward physical activity. Attitudes about physical activity rather than personality traits were shown to be important within the subpopulation studied.", "contents": "Attitude and personality differences in physically active and inactive university teachers. This study assessed peronality factors and attitudes toward physical activity in a sample of university teachers. One group (N = 20) of subjects regularly participated in various forms of physical activity; the second (N = 20) showed no such interest. The psychological measures used were the Cattell 16 PF Form A and the Kenyon questionnaire on attitudes toward physical activity. Attitudes about physical activity rather than personality traits were shown to be important within the subpopulation studied.", "PMID": 980649}
{"id": "pubmed23n0032_12469", "title": "Object, intensity and subject variables in visual evoked response.", "content": "10 subjects were studied to determine AEP effects of square, circle, and blank stimuli with variable stimulus intensity. For the group as a whole, object and intensity effects were significant on a number of amplitude and latency measures, but the object effect appears largely to reflect the presence or absence of a figure, rather than its nature. Increased intensity differentially affected latency, shortening the first negative deflection while lengthening the second positive. Amplitude is generally increased with higher intensity. Analysis of within-subjects effects showed considerable variability as to which parameters were significantly related to the independent variables in individual subjects. A repeated-measures, within-subjects research strategy is seen as appropriate on the basis of these data.", "contents": "Object, intensity and subject variables in visual evoked response. 10 subjects were studied to determine AEP effects of square, circle, and blank stimuli with variable stimulus intensity. For the group as a whole, object and intensity effects were significant on a number of amplitude and latency measures, but the object effect appears largely to reflect the presence or absence of a figure, rather than its nature. Increased intensity differentially affected latency, shortening the first negative deflection while lengthening the second positive. Amplitude is generally increased with higher intensity. Analysis of within-subjects effects showed considerable variability as to which parameters were significantly related to the independent variables in individual subjects. A repeated-measures, within-subjects research strategy is seen as appropriate on the basis of these data.", "PMID": 980650}
{"id": "pubmed23n0032_12470", "title": "Influence of recall on reliability of perception of the autokinetic illusion.", "content": "An autokinetic test was given to 76 females subjects who were then randomly assigned to 1 of 4 groups of 19 subjects each. Two groups were retested after 1 and 6 wk., respectively. The other two groups were asked to recall their initial experience after 1 and 6 wk., respectively. Intertrial correlations of the measures were generally higher for the recall conditions than for the retest conditions. The results were discussed in terms of the potential influence that recall may have on the test-retest reliability of autokinesis.", "contents": "Influence of recall on reliability of perception of the autokinetic illusion. An autokinetic test was given to 76 females subjects who were then randomly assigned to 1 of 4 groups of 19 subjects each. Two groups were retested after 1 and 6 wk., respectively. The other two groups were asked to recall their initial experience after 1 and 6 wk., respectively. Intertrial correlations of the measures were generally higher for the recall conditions than for the retest conditions. The results were discussed in terms of the potential influence that recall may have on the test-retest reliability of autokinesis.", "PMID": 980651}
{"id": "pubmed23n0032_12471", "title": "Empathy in learning disabled children.", "content": "25 7- to 12-yr.-old boys with visual perceptual problems and learning disabilities were compared on the Borke Scales for Empathy with a greater matched control group. The learning disabled group had significantly greater difficulty recognizing and labeling emotions.", "contents": "Empathy in learning disabled children. 25 7- to 12-yr.-old boys with visual perceptual problems and learning disabilities were compared on the Borke Scales for Empathy with a greater matched control group. The learning disabled group had significantly greater difficulty recognizing and labeling emotions.", "PMID": 980652}
{"id": "pubmed23n0032_12472", "title": "Sex cues in estimating and performing a simple motor task.", "content": "The present study examined direct behavioral effects of sex cues in a modified level of aspiration experiment for 60 male and 60 female undergraduates. Each S was provided a constant referent score (25), identified as the average score, average score for men, or average score for women. E's sex was controlled and its effects examined. Male Ss estimated and performed significantly higher than female Ss. All Ss provided the average score for the men estimated and performed lower than Ss provided the average score for women. Effect of E's sex was nonsignificant. Results were discussed in terms of behavioral effects of sex-role stereotypes and the implications of such effects for future research.", "contents": "Sex cues in estimating and performing a simple motor task. The present study examined direct behavioral effects of sex cues in a modified level of aspiration experiment for 60 male and 60 female undergraduates. Each S was provided a constant referent score (25), identified as the average score, average score for men, or average score for women. E's sex was controlled and its effects examined. Male Ss estimated and performed significantly higher than female Ss. All Ss provided the average score for the men estimated and performed lower than Ss provided the average score for women. Effect of E's sex was nonsignificant. Results were discussed in terms of behavioral effects of sex-role stereotypes and the implications of such effects for future research.", "PMID": 980653}
{"id": "pubmed23n0032_12473", "title": "Heterosexual social competence, anxiety, avoidance and self-judged physical attractiveness.", "content": "The relationship between self-judged physical attractiveness and opposite-sex behavior was examined as part of a large survey on the interaction patterns of 963 college students. The findings suggest that a self-rated negative physical image is related to significantly high levels of heterosexual difficulties.", "contents": "Heterosexual social competence, anxiety, avoidance and self-judged physical attractiveness. The relationship between self-judged physical attractiveness and opposite-sex behavior was examined as part of a large survey on the interaction patterns of 963 college students. The findings suggest that a self-rated negative physical image is related to significantly high levels of heterosexual difficulties.", "PMID": 980654}
{"id": "pubmed23n0032_12474", "title": "Information processing vs visual attention during incidental learning.", "content": "The relationship between attention and incidental learning during discrimination training was studied in 30 children, aged 10 to 11. A polymetric eye-movement recorder measured direct visual attention. Consistent with previous findings, recall of incidental stimuli was greatest during the initial and terminal stages of intentional learning. Contrary to previous explanations, however, visual attention to incidental stimuli was not related to training. While individual differences in attention to incidental stimuli were predictive of recall, attention to incidental stimuli was not related to level of training. Results suggested that changes in higher order information processing rather than direct visual attention were responsible for the curvilinear learning of incidental stimuli during intentional training.", "contents": "Information processing vs visual attention during incidental learning. The relationship between attention and incidental learning during discrimination training was studied in 30 children, aged 10 to 11. A polymetric eye-movement recorder measured direct visual attention. Consistent with previous findings, recall of incidental stimuli was greatest during the initial and terminal stages of intentional learning. Contrary to previous explanations, however, visual attention to incidental stimuli was not related to training. While individual differences in attention to incidental stimuli were predictive of recall, attention to incidental stimuli was not related to level of training. Results suggested that changes in higher order information processing rather than direct visual attention were responsible for the curvilinear learning of incidental stimuli during intentional training.", "PMID": 980655}
{"id": "pubmed23n0032_12475", "title": "Effects of prolonged practice on duration of spiral aftereffect: an ideographic study.", "content": "The effects of prolonged practice on the duration of the spiral aftereffect were investigated; 3,000 trials over 30 days with 5 levels of ambient illumination were randomly presented to a single subject. Scores gradually decreased until a plateau was reached during the last 13 days. A large decrement during the last 5 days was attributed to a decision process component. An early difference in performance at different illumination levels was largely eliminated by the last 5 days.", "contents": "Effects of prolonged practice on duration of spiral aftereffect: an ideographic study. The effects of prolonged practice on the duration of the spiral aftereffect were investigated; 3,000 trials over 30 days with 5 levels of ambient illumination were randomly presented to a single subject. Scores gradually decreased until a plateau was reached during the last 13 days. A large decrement during the last 5 days was attributed to a decision process component. An early difference in performance at different illumination levels was largely eliminated by the last 5 days.", "PMID": 980656}
{"id": "pubmed23n0032_12476", "title": "WISC characteristics of reading disabled children identified by three objective methods.", "content": "The WISC and Spache Reading Diagnostic Scales were administered to 119 third-grade children. Groups of reading disabled children were selected by three objective methods (Below Grade Level, Bond and Tinker, and the Erickson Z-score Discrepancy). The Below Grade Level and Bond and Tinker methods tended to identify the same children whose IQs and reading achievement scores were below average. The Z-score method identified children with IQs at or above the group average. Comparison of the poor readers with control groups matched for IQ and sex gave only minimal evidence for WISC subtest patterns as characteristic of poor readers.", "contents": "WISC characteristics of reading disabled children identified by three objective methods. The WISC and Spache Reading Diagnostic Scales were administered to 119 third-grade children. Groups of reading disabled children were selected by three objective methods (Below Grade Level, Bond and Tinker, and the Erickson Z-score Discrepancy). The Below Grade Level and Bond and Tinker methods tended to identify the same children whose IQs and reading achievement scores were below average. The Z-score method identified children with IQs at or above the group average. Comparison of the poor readers with control groups matched for IQ and sex gave only minimal evidence for WISC subtest patterns as characteristic of poor readers.", "PMID": 980657}
{"id": "pubmed23n0032_12477", "title": "Reaction time as a function of peripheral retinal locus around fovea: effect of stimulus size.", "content": "Using targets of four sizes between 18' and 116' at a fixed luminance of 5.8 cd/m2, human visual reaction times (RT) were measured on a circle at 30 degrees eccentric to the fovea. A foveal and 12 peripheral retinal loci were explored, covering the range between 0 degrees and 330 degrees in steps of 30 degrees units on a circle about the fovea. RT decreased significantly by the stimulation to the quadrant retinal loci between nasal and superior side. RT decreased as a function of increasing target size and the amount of decrement was relatively larger in the periphery than in the fovea.", "contents": "Reaction time as a function of peripheral retinal locus around fovea: effect of stimulus size. Using targets of four sizes between 18' and 116' at a fixed luminance of 5.8 cd/m2, human visual reaction times (RT) were measured on a circle at 30 degrees eccentric to the fovea. A foveal and 12 peripheral retinal loci were explored, covering the range between 0 degrees and 330 degrees in steps of 30 degrees units on a circle about the fovea. RT decreased significantly by the stimulation to the quadrant retinal loci between nasal and superior side. RT decreased as a function of increasing target size and the amount of decrement was relatively larger in the periphery than in the fovea.", "PMID": 980658}
{"id": "pubmed23n0032_12478", "title": "Note on auditory perception of physiognomic properties in short melodic structures.", "content": "The present study shows that short melodies composed to conform with the Gestalt features of line-schematizations which had been drawn to be expressive of certain emotions and concepts were, in turn, judged by both 28 blind boys and girls and 87 college students sighted subjects, according to prediction. The results are interpreted as supporting the underlying hypothesis that physiognomic properties are \"similar\" across modalities.", "contents": "Note on auditory perception of physiognomic properties in short melodic structures. The present study shows that short melodies composed to conform with the Gestalt features of line-schematizations which had been drawn to be expressive of certain emotions and concepts were, in turn, judged by both 28 blind boys and girls and 87 college students sighted subjects, according to prediction. The results are interpreted as supporting the underlying hypothesis that physiognomic properties are \"similar\" across modalities.", "PMID": 980659}
{"id": "pubmed23n0032_12479", "title": "Subjective definitions of tension.", "content": "To examine the type of proprioceptive information which subjects include in the definition of tension, 52 subjects were asked to indicate the location of where they felt tension while engaging in a simple motor task. Responses indicated that tendon stretch, muscle tension, and other cues are utilized in the subject's definition of tension.", "contents": "Subjective definitions of tension. To examine the type of proprioceptive information which subjects include in the definition of tension, 52 subjects were asked to indicate the location of where they felt tension while engaging in a simple motor task. Responses indicated that tendon stretch, muscle tension, and other cues are utilized in the subject's definition of tension.", "PMID": 980660}
{"id": "pubmed23n0032_12480", "title": "Practice effects for auditory localization: a test of a differentiation theory of perceptual learning and development.", "content": "Practice and transfer of practice in an auditory localization task were observed for three different information conditions (normal, transformed and reduced) to examine four aspects of Gibson's (1969) differentiation theory of perceptual learning and development. Support was found for the propositions that: (i) dimensions of difference, rather than prototypes, are learned; (ii) specificity is a more appropriate response measure than number of correct responses; (iii) perceptual learning will occur regardless of whether feedback is given or not; (iv) transfer of practice will be greater if dimensions of difference (as opposed to prototypes) are learned. It was also found that feedback during practice did not affect transfer. In an apparent contradiction though, specificity increased in the absence of dimension of difference. This increase was interpreted as being associated with the detection of non-task-related information and was seen as lending support to Gibson's hypothesis that, perceptual learning is motivated by intrinsic cognitive drive and is terminated by a reduction in subjective uncertainty.", "contents": "Practice effects for auditory localization: a test of a differentiation theory of perceptual learning and development. Practice and transfer of practice in an auditory localization task were observed for three different information conditions (normal, transformed and reduced) to examine four aspects of Gibson's (1969) differentiation theory of perceptual learning and development. Support was found for the propositions that: (i) dimensions of difference, rather than prototypes, are learned; (ii) specificity is a more appropriate response measure than number of correct responses; (iii) perceptual learning will occur regardless of whether feedback is given or not; (iv) transfer of practice will be greater if dimensions of difference (as opposed to prototypes) are learned. It was also found that feedback during practice did not affect transfer. In an apparent contradiction though, specificity increased in the absence of dimension of difference. This increase was interpreted as being associated with the detection of non-task-related information and was seen as lending support to Gibson's hypothesis that, perceptual learning is motivated by intrinsic cognitive drive and is terminated by a reduction in subjective uncertainty.", "PMID": 980661}
{"id": "pubmed23n0032_12481", "title": "Personality dysfunction and lateralized deficits in cerebral functions as measured by the MMPI and Reitan-Halstead Battery.", "content": "Previous research using the MMPI has failed to show personality differences between right and left hemispheric brain-damaged patients. The purposes of the present study were, first to determine whether lateralized deficits in cortical functioning as measured by neuropsychological testing are systematically associated with neurotic-psychotic distinctions on the MMPI. Secondly, we wished to determine the strength of predictive association in the relationship between lateralized functional deficits and personality organization. A localization key (Russell, et al., 1972) was applied to the \"Reitan-Battery\" test results for 94 patients who were referred for neuropsychological testing. Based on this approach, 15 pairs of patients were selected who were \"left\" or \"right\" hemispheric dysfunctional in terms of test performance. Pairs were  matched for age and \"average impairment rating\" (Russell, et al., 1972). Their MMPIs were also designated \"neurotic\" or \"psychotic\" using the Goldberg Psychotic Index (Goldberg, 1972). The results suggest that patients with deficits in left hemisphere functions tend to score in the psychotic range and patients with deficits in right hemispheric functions tend to score in the neurotic range of the MMPI Goldberg Index. In this set of data there was a 38% reduction in error of predicting neurotic-psychotic category but knowing whether the patient was left or right hemispheric dysfunctional in terms of neuropsychological tests.", "contents": "Personality dysfunction and lateralized deficits in cerebral functions as measured by the MMPI and Reitan-Halstead Battery. Previous research using the MMPI has failed to show personality differences between right and left hemispheric brain-damaged patients. The purposes of the present study were, first to determine whether lateralized deficits in cortical functioning as measured by neuropsychological testing are systematically associated with neurotic-psychotic distinctions on the MMPI. Secondly, we wished to determine the strength of predictive association in the relationship between lateralized functional deficits and personality organization. A localization key (Russell, et al., 1972) was applied to the \"Reitan-Battery\" test results for 94 patients who were referred for neuropsychological testing. Based on this approach, 15 pairs of patients were selected who were \"left\" or \"right\" hemispheric dysfunctional in terms of test performance. Pairs were  matched for age and \"average impairment rating\" (Russell, et al., 1972). Their MMPIs were also designated \"neurotic\" or \"psychotic\" using the Goldberg Psychotic Index (Goldberg, 1972). The results suggest that patients with deficits in left hemisphere functions tend to score in the psychotic range and patients with deficits in right hemispheric functions tend to score in the neurotic range of the MMPI Goldberg Index. In this set of data there was a 38% reduction in error of predicting neurotic-psychotic category but knowing whether the patient was left or right hemispheric dysfunctional in terms of neuropsychological tests.", "PMID": 980663}
{"id": "pubmed23n0032_12482", "title": "Effect of item arrangement on perceived numerosity: randomness vs regularity.", "content": "Subjects were shown pairs of dot patterns, a regular one and a randomly arranged set, and were asked to say which appeared more numerous. In three experiments using 53 college students regular patterns appeared significantly more numerous than random arrays, with an average illusion of 5.5%. The results were discussed in relation to the breakdown of conservation of number.", "contents": "Effect of item arrangement on perceived numerosity: randomness vs regularity. Subjects were shown pairs of dot patterns, a regular one and a randomly arranged set, and were asked to say which appeared more numerous. In three experiments using 53 college students regular patterns appeared significantly more numerous than random arrays, with an average illusion of 5.5%. The results were discussed in relation to the breakdown of conservation of number.", "PMID": 980664}
{"id": "pubmed23n0032_12483", "title": "Instructional control of sleep reduction.", "content": "Nightly sleep of 8 college students was gradually reduced from baseline levels by instructions implemented in a multiple baseline, changing criterion design. The reduction phases were 5%, 15%, and 30% decreases. Performance, academic, and sleepiness measures were collected. Consistent reductions occurred for all subjects from mean daily sleep times of 7.71 to 6.20 hr. per night, a 20% decrease. No negative side-effects were observed and subjects reported they enjoyed the additional free time afforded by reducing their sleep.", "contents": "Instructional control of sleep reduction. Nightly sleep of 8 college students was gradually reduced from baseline levels by instructions implemented in a multiple baseline, changing criterion design. The reduction phases were 5%, 15%, and 30% decreases. Performance, academic, and sleepiness measures were collected. Consistent reductions occurred for all subjects from mean daily sleep times of 7.71 to 6.20 hr. per night, a 20% decrease. No negative side-effects were observed and subjects reported they enjoyed the additional free time afforded by reducing their sleep.", "PMID": 980665}
{"id": "pubmed23n0032_12484", "title": "Three studies of personality: undergraduate students in physical education.", "content": "In Study I, 85 male undergraduate physical education majors were compared to the norms on the 1962 16 PF test; Ss were higher in ego strength, dominant, enthusiastic, practical, naive, self-assured, and group-dependent. In Study II, 50 similar Ss took the 1967 test and were lower in intelligence, higher in superego strength, and were more practical and controlled. In Study III, 39 similar female Ss took the 1967 test and were lower in intelligence, reserved, practical, and conservative. Centiles calculated from the raw scores showed the groups deviated from the normative groups by 15 points or more on some of these traits. Findings generally agree with previous work using the same instrument and disagree with common conclusions regarding fitness for teaching by other investigators using different instruments.", "contents": "Three studies of personality: undergraduate students in physical education. In Study I, 85 male undergraduate physical education majors were compared to the norms on the 1962 16 PF test; Ss were higher in ego strength, dominant, enthusiastic, practical, naive, self-assured, and group-dependent. In Study II, 50 similar Ss took the 1967 test and were lower in intelligence, higher in superego strength, and were more practical and controlled. In Study III, 39 similar female Ss took the 1967 test and were lower in intelligence, reserved, practical, and conservative. Centiles calculated from the raw scores showed the groups deviated from the normative groups by 15 points or more on some of these traits. Findings generally agree with previous work using the same instrument and disagree with common conclusions regarding fitness for teaching by other investigators using different instruments.", "PMID": 980666}
{"id": "pubmed23n0032_12485", "title": "The perception of familiar stimuli.", "content": "A model is described for the processing of word-like (regular) and non-word-like (irregular) letter strings in a situation where either type of string may occur. It is proposed that each type of string is processed by specialised routines which are deployed contingently on an initial decision about the regularity of an item. Three experiments involving word/nonword and same/different responses investigate how the processing of a string is affected by the brief exposure of a prior string. It is shown that processing is interfered with less when both strings are of the same degree of regularity than when they are not. The model is revised and extended in the light of the results obtained as well as some theoretical considerations.", "contents": "The perception of familiar stimuli. A model is described for the processing of word-like (regular) and non-word-like (irregular) letter strings in a situation where either type of string may occur. It is proposed that each type of string is processed by specialised routines which are deployed contingently on an initial decision about the regularity of an item. Three experiments involving word/nonword and same/different responses investigate how the processing of a string is affected by the brief exposure of a prior string. It is shown that processing is interfered with less when both strings are of the same degree of regularity than when they are not. The model is revised and extended in the light of the results obtained as well as some theoretical considerations.", "PMID": 980667}
{"id": "pubmed23n0032_12486", "title": "The contribution of relational factors to line-length matches.", "content": "Both Kunnapas and Rock and Ebenholtz investigated the effects of surrounding-frame size upon line-length judgments. Whereas Kunnapas obtained errors of the order of 10-15%, typical of those which occur in other geometrical illusion figures, Rock and Ebenholtz reported errors closer to 100%. Rock and Ebenholtz claimed that perceived size is largely relationally determined and that this fact was obscured in Kubnapas experiment by allowing observers to compare the test lines directly with each other and within a common framework provided by the wall of the room. On the contrary, the experiments reported here suggested that errors in the Rock and Ebenholtz study may have been inflated by their use of nondirective instructions and by the confounded effects of other variables. When the Rock and Ebenholtz experiments were repeated with adequat controls over these variables, the effect of surrounding frames on line-length matches were of the order of 15-19%, similar to those of Kunnapas but much smaller than those reported by Rock and Ebenholtz. The complexity of the stimuli in the Rock and Ebenholtz type of experiment is such, however, that the effects of a number of variables and possible cue conflicts remain to be investigated.", "contents": "The contribution of relational factors to line-length matches. Both Kunnapas and Rock and Ebenholtz investigated the effects of surrounding-frame size upon line-length judgments. Whereas Kunnapas obtained errors of the order of 10-15%, typical of those which occur in other geometrical illusion figures, Rock and Ebenholtz reported errors closer to 100%. Rock and Ebenholtz claimed that perceived size is largely relationally determined and that this fact was obscured in Kubnapas experiment by allowing observers to compare the test lines directly with each other and within a common framework provided by the wall of the room. On the contrary, the experiments reported here suggested that errors in the Rock and Ebenholtz study may have been inflated by their use of nondirective instructions and by the confounded effects of other variables. When the Rock and Ebenholtz experiments were repeated with adequat controls over these variables, the effect of surrounding frames on line-length matches were of the order of 15-19%, similar to those of Kunnapas but much smaller than those reported by Rock and Ebenholtz. The complexity of the stimuli in the Rock and Ebenholtz type of experiment is such, however, that the effects of a number of variables and possible cue conflicts remain to be investigated.", "PMID": 980668}
{"id": "pubmed23n0032_12487", "title": "Away from a unisex psychology: individual differences in visual sensory and perceptual processes.", "content": "Four tests of visual perception were given to twenty-five men and twenty-five women. These were a test of acuity, threshold for four field positions, visual persistence, and a measure of comfortable brightness. Subjects also completed five personality questionnaires. In most measures, differences were found to be related to sex rather than to personality factors. In fact, the analyses performed suggest that persenality tests do not measure equivalent processes in men and women. Correlational anaylsis showed all visual functions to be independent of one another with the exception of photopic acuity and scotopic threshold, which were highly correlated. Two new findings on the visual system emerged which have not been reported elsewhere: (i) Four distinct dark adaptation curves were produced, and have been labeled as exponential, flat-exponential, linear, and plateau. All subjects fell into one of these categories and showed a consistent trend to exhibit these curves for all field positions. (ii) Highly significant differences were found in sensitivity for the four visual fields, the upper field was superior, followed by the right, then left, with the lower visual field considerably poorer.", "contents": "Away from a unisex psychology: individual differences in visual sensory and perceptual processes. Four tests of visual perception were given to twenty-five men and twenty-five women. These were a test of acuity, threshold for four field positions, visual persistence, and a measure of comfortable brightness. Subjects also completed five personality questionnaires. In most measures, differences were found to be related to sex rather than to personality factors. In fact, the analyses performed suggest that persenality tests do not measure equivalent processes in men and women. Correlational anaylsis showed all visual functions to be independent of one another with the exception of photopic acuity and scotopic threshold, which were highly correlated. Two new findings on the visual system emerged which have not been reported elsewhere: (i) Four distinct dark adaptation curves were produced, and have been labeled as exponential, flat-exponential, linear, and plateau. All subjects fell into one of these categories and showed a consistent trend to exhibit these curves for all field positions. (ii) Highly significant differences were found in sensitivity for the four visual fields, the upper field was superior, followed by the right, then left, with the lower visual field considerably poorer.", "PMID": 980669}
{"id": "pubmed23n0032_12488", "title": "Sex differences in visual persistence: experiments on the Ganzfeld and afterimages.", "content": "Sex differences were investigated in two experiments on visual persistence: the Ganzfeld and the afterimage. Males were found to hold visual sensation longer than females, particularly in the Ganzfeld where there was little overlap of scores. Variability of experience in the Ganzfeld was also greater for males and they commonly reported 'blank-out' effects while females did not. There was further evidence from both experiments that females are more responsive to the long-wave region of the frequency spectrum.", "contents": "Sex differences in visual persistence: experiments on the Ganzfeld and afterimages. Sex differences were investigated in two experiments on visual persistence: the Ganzfeld and the afterimage. Males were found to hold visual sensation longer than females, particularly in the Ganzfeld where there was little overlap of scores. Variability of experience in the Ganzfeld was also greater for males and they commonly reported 'blank-out' effects while females did not. There was further evidence from both experiments that females are more responsive to the long-wave region of the frequency spectrum.", "PMID": 980670}
{"id": "pubmed23n0032_12489", "title": "Striate cortex potentials related to eye movements in the light and in darkness in the waking human.", "content": "Potentials in relation to eye movements were studied by means of direct recording of the striate cortex in a waking man. In a lighted environment, the usual evoked potential--lambda response--was obtained and was clearly visible after each eye movement. In complete darkness no individual potential was observable by means of visual analysis after each eye movement, but a slow potential of low amplitude could be obtained by superimposition and averaging of the cortical striate activity time-locked to the start of a series of eye movements. This eye-movement potential showed a longer latency and a lower amplitude than the lambda response. These data are discussed in reference to those obtained in the cat and the monkey; the significance of this eye-movement potential in darkness as a 'corollary discharge' is considered.", "contents": "Striate cortex potentials related to eye movements in the light and in darkness in the waking human. Potentials in relation to eye movements were studied by means of direct recording of the striate cortex in a waking man. In a lighted environment, the usual evoked potential--lambda response--was obtained and was clearly visible after each eye movement. In complete darkness no individual potential was observable by means of visual analysis after each eye movement, but a slow potential of low amplitude could be obtained by superimposition and averaging of the cortical striate activity time-locked to the start of a series of eye movements. This eye-movement potential showed a longer latency and a lower amplitude than the lambda response. These data are discussed in reference to those obtained in the cat and the monkey; the significance of this eye-movement potential in darkness as a 'corollary discharge' is considered.", "PMID": 980671}
{"id": "pubmed23n0032_12490", "title": "The aftereffect of tracking eye movements.", "content": "When observers tracked moving stripes across a background either of stationary stripes, or of stripes moving in the opposite direction, they saw a clear motion aftereffect when the stripes stopped moving. The direction of this aftereffect was opposite to that of the previously tracked stripes, and was thus the same as the direction of the retinal movement of the non-tracked stripes. This aftereffect of tracking was shown not to depend upon slippage of the tracked contours on the retina during tracking, or upon the saccadic phase of optokinetic nystagmus. The effect showed storage over a period of time with the eyes shut. It appears that the effect is due to induced movement, and arises originally from stimulation of the retina by background contours in the tracking phase. This was shown by confining the view of the moving target to one eye, while permitting both eyes to be exposed to background stimulation during tracking. After such stimulation the magnitude of the aftereffect was equal in the two eyes.", "contents": "The aftereffect of tracking eye movements. When observers tracked moving stripes across a background either of stationary stripes, or of stripes moving in the opposite direction, they saw a clear motion aftereffect when the stripes stopped moving. The direction of this aftereffect was opposite to that of the previously tracked stripes, and was thus the same as the direction of the retinal movement of the non-tracked stripes. This aftereffect of tracking was shown not to depend upon slippage of the tracked contours on the retina during tracking, or upon the saccadic phase of optokinetic nystagmus. The effect showed storage over a period of time with the eyes shut. It appears that the effect is due to induced movement, and arises originally from stimulation of the retina by background contours in the tracking phase. This was shown by confining the view of the moving target to one eye, while permitting both eyes to be exposed to background stimulation during tracking. After such stimulation the magnitude of the aftereffect was equal in the two eyes.", "PMID": 980672}
{"id": "pubmed23n0032_12491", "title": "The perpendicular error and the vertical effect in children's drawing.", "content": "It has been known for some time that children have particular difficulty drawing lines that are not perpendicular. But this difficulty has not been studied systematically. By systematically varying, in three experiments, the baseline, the response, and the kind of figures to be copied, we showed that the tendency to draw angles as more perpendicular than they actually are is a general one which occurs as much with abstract as with meaningful material and with radically different responses. We also discovered an unexpected effect, the vertical effect, which takes the form of the error occurring much less when a vertical baseline is involved than with other baselines.", "contents": "The perpendicular error and the vertical effect in children's drawing. It has been known for some time that children have particular difficulty drawing lines that are not perpendicular. But this difficulty has not been studied systematically. By systematically varying, in three experiments, the baseline, the response, and the kind of figures to be copied, we showed that the tendency to draw angles as more perpendicular than they actually are is a general one which occurs as much with abstract as with meaningful material and with radically different responses. We also discovered an unexpected effect, the vertical effect, which takes the form of the error occurring much less when a vertical baseline is involved than with other baselines.", "PMID": 980673}
{"id": "pubmed23n0032_12492", "title": "The magic number 4 +/- 0: a new look at visual numerosity judgements.", "content": "Visual numerosity judgements were made for tachistoscopically presented linear arrays of dots or lines. The interelement interval (which could be specified in spatial frequency terms) was constant for a given array but varied across conditions. A clear limit in the accuracy of numerosity judgements was found to be set at 4 for regularly spaced elements with spatial frequencies below approximately 10 cycles/deg (element and interelement interval of 0-05 deg). This limit in terms of accuracy is accompanied by a fast and almost constant response time for arrays of 4 or less, compared to response times for arrays of more than 4 elements. The limit in accuracy falls to 2 elements rather than 4 for spacing narrower than 0-05 deg alghough with such spacing the elements are still easily resolved. The limit of 4 is found if the stimulus is a bright afterimage, lasting for approximately 60 s. This result suggests that the limit is independent of the time allowed for a single fixation and is a perceptual limit rather than a limit in some memory buffer. 'Numerosity' units are propsoed to account for the results.", "contents": "The magic number 4 +/- 0: a new look at visual numerosity judgements. Visual numerosity judgements were made for tachistoscopically presented linear arrays of dots or lines. The interelement interval (which could be specified in spatial frequency terms) was constant for a given array but varied across conditions. A clear limit in the accuracy of numerosity judgements was found to be set at 4 for regularly spaced elements with spatial frequencies below approximately 10 cycles/deg (element and interelement interval of 0-05 deg). This limit in terms of accuracy is accompanied by a fast and almost constant response time for arrays of 4 or less, compared to response times for arrays of more than 4 elements. The limit in accuracy falls to 2 elements rather than 4 for spacing narrower than 0-05 deg alghough with such spacing the elements are still easily resolved. The limit of 4 is found if the stimulus is a bright afterimage, lasting for approximately 60 s. This result suggests that the limit is independent of the time allowed for a single fixation and is a perceptual limit rather than a limit in some memory buffer. 'Numerosity' units are propsoed to account for the results.", "PMID": 980674}
{"id": "pubmed23n0032_12493", "title": "The dependence of the visual numerosity limit on orientation, colour, and grouping in the stimulus.", "content": "Visual numerosity judgements were made for tachistoscopically presented arrays of dots. The arrangement within the arrays was either linear or such that dots could be easily perceptually subdivided into two groups. Subdivision was either in terms of an orientation difference, a colour difference, or a spacing difference in the centre of the array. For a large difference in orientation between the two 'arms' of the array (90 degrees), or a large central space (three times the interdot interval) up to 8 dots were accurately perceived. This numerosity limit was twice that found for equivalent linear arrays, with no grouping. Although in terms of accuracy it seems that in these conditions the two groups within each array can be counted independently, there is no evidence for independent processing in terms of response times. From the results of a subsidiary experiment it seems likely that the slow response times in the subgrouping conditions are due to the necessity of processes other than counting (such as judgements of symmetry). For arrays where subgrouping was in teems of a colour difference, or an orientation difference of between approximately 45 degrees and 90 degrees, or a small central space of twice the interdot interval, there was an improvement in accuracy compared to equivalent linear arrays, but no evidence of independent processing, up to a limit of 4, in each of the subgroups. From these preliminary results, tentative proposals concerning 'numerosity' units and their properties are made.", "contents": "The dependence of the visual numerosity limit on orientation, colour, and grouping in the stimulus. Visual numerosity judgements were made for tachistoscopically presented arrays of dots. The arrangement within the arrays was either linear or such that dots could be easily perceptually subdivided into two groups. Subdivision was either in terms of an orientation difference, a colour difference, or a spacing difference in the centre of the array. For a large difference in orientation between the two 'arms' of the array (90 degrees), or a large central space (three times the interdot interval) up to 8 dots were accurately perceived. This numerosity limit was twice that found for equivalent linear arrays, with no grouping. Although in terms of accuracy it seems that in these conditions the two groups within each array can be counted independently, there is no evidence for independent processing in terms of response times. From the results of a subsidiary experiment it seems likely that the slow response times in the subgrouping conditions are due to the necessity of processes other than counting (such as judgements of symmetry). For arrays where subgrouping was in teems of a colour difference, or an orientation difference of between approximately 45 degrees and 90 degrees, or a small central space of twice the interdot interval, there was an improvement in accuracy compared to equivalent linear arrays, but no evidence of independent processing, up to a limit of 4, in each of the subgroups. From these preliminary results, tentative proposals concerning 'numerosity' units and their properties are made.", "PMID": 980675}
{"id": "pubmed23n0032_12494", "title": "Implicit-shape constancy: a cross-cultural comparison.", "content": "A group of Scottish schoolchildren were tested on a task intended to measure the effect of implicit-shape constancy, and the scores were compared with those obtained from African samples. It was found that both groups were influenced by the implicit-shape constancy although the influence was less in the African sample. The relationship of these findings to other published reports of cross-cultural research into pictorial perception and susceptibility to illusions is discussed.", "contents": "Implicit-shape constancy: a cross-cultural comparison. A group of Scottish schoolchildren were tested on a task intended to measure the effect of implicit-shape constancy, and the scores were compared with those obtained from African samples. It was found that both groups were influenced by the implicit-shape constancy although the influence was less in the African sample. The relationship of these findings to other published reports of cross-cultural research into pictorial perception and susceptibility to illusions is discussed.", "PMID": 980676}
{"id": "pubmed23n0032_12495", "title": "Model-driven interpretation in intelligent vision systems.", "content": "With a constructive knowledge-based theory of perception as its foundation, this paper starts with a review and critique of some artificial-intelligence programs that purport to see. It is then argued that these computer programs for scene analysis offer the hope of providing a more adequate account oo the current psychological theories. This thesis has several aspects. The one emphasized here is that those programs have explored a variety of methods of incorporating a priori knowledge of objects through the use of models. After outlining the range of models used, presenting a set of criteria for evaluating the use of model information, and sketching some psychological theories, the various proposals are contrasted. This discussion leads to two new proposals for exploiting model information that involve elaborations of an existing program, POLY.", "contents": "Model-driven interpretation in intelligent vision systems. With a constructive knowledge-based theory of perception as its foundation, this paper starts with a review and critique of some artificial-intelligence programs that purport to see. It is then argued that these computer programs for scene analysis offer the hope of providing a more adequate account oo the current psychological theories. This thesis has several aspects. The one emphasized here is that those programs have explored a variety of methods of incorporating a priori knowledge of objects through the use of models. After outlining the range of models used, presenting a set of criteria for evaluating the use of model information, and sketching some psychological theories, the various proposals are contrasted. This discussion leads to two new proposals for exploiting model information that involve elaborations of an existing program, POLY.", "PMID": 980677}
{"id": "pubmed23n0032_12496", "title": "Pituitary dwarfism in German shepherd dogs: additional evidence of simple, autosomal recessive inheritance.", "content": "Previous observations suggested that pituitary dwarfism of German Shepherd dogs is due to simple, autosomal recessive inheritance, but own data were insufficient to test the hypothesis. The total material now includes six dwarfs from five litters. Omitting the proband litter, a total of 21 dogs from four litters, each with one dwarf, were relevant for testing goodness of fit. The observed segregation ratio was in agreement with that expected and thus supported the genetic hypothesis. According to this hypothesis it was possible to point at various heterozygous carriers among ancestors of the dwarfs within a pedigree comprising 13 generations.", "contents": "Pituitary dwarfism in German shepherd dogs: additional evidence of simple, autosomal recessive inheritance. Previous observations suggested that pituitary dwarfism of German Shepherd dogs is due to simple, autosomal recessive inheritance, but own data were insufficient to test the hypothesis. The total material now includes six dwarfs from five litters. Omitting the proband litter, a total of 21 dogs from four litters, each with one dwarf, were relevant for testing goodness of fit. The observed segregation ratio was in agreement with that expected and thus supported the genetic hypothesis. According to this hypothesis it was possible to point at various heterozygous carriers among ancestors of the dwarfs within a pedigree comprising 13 generations.", "PMID": 980693}
{"id": "pubmed23n0032_12497", "title": "Lung lesions at slaughter: associations to factors in the pig herd.", "content": "A survey was made of available data files to gain a preliminary insight into the association between environmental factors and the prevalence at slaughter of lung lesions. Herd size was the factor that showed the most predominant association with the prevalence of lung lesions. This was largely independent of the recruitment system for weanlings. There was a moderate trend toward positive association between the prevalence of lung lesions and the prevalence of other lesions. Lung lesions were weakly but significantly associated with the occurrence of infectious diarrhoea in the herd. The investigation demonstrated the feasibility of and the need for continuous epidemiological studies to identify disease determinants. Studies of the nature reported here makes it possible to define essential health factors with an accuracy that could never be achieved through clinical observations in a few herds.", "contents": "Lung lesions at slaughter: associations to factors in the pig herd. A survey was made of available data files to gain a preliminary insight into the association between environmental factors and the prevalence at slaughter of lung lesions. Herd size was the factor that showed the most predominant association with the prevalence of lung lesions. This was largely independent of the recruitment system for weanlings. There was a moderate trend toward positive association between the prevalence of lung lesions and the prevalence of other lesions. Lung lesions were weakly but significantly associated with the occurrence of infectious diarrhoea in the herd. The investigation demonstrated the feasibility of and the need for continuous epidemiological studies to identify disease determinants. Studies of the nature reported here makes it possible to define essential health factors with an accuracy that could never be achieved through clinical observations in a few herds.", "PMID": 980694}
{"id": "pubmed23n0032_12498", "title": "Zinc as a possible causal factor in the sterilizing sperm tail defect, the 'Dag-defect', in Jersey bulls.", "content": "The zinc content was determined in semen samples from four young Danish Jersey bulls showing the typical 'Dag defect' (greater than 50% strongly coiled or bent sperm tails). Two of the bulls were half brothers. Semen samples from 14 normal fertile Jersey bulls served as controls. The normal material showed a zinc content in the centrifuged sperm of 190 +/- 15.8 mug/g d.w. and in the seminal plasma 81 +/- 31.2 mug/g d.w. In the four abnormal bulls the following elevated values were found: in the centrifuged sperm 310 +/- 109.5 mug/g d.w. and in the seminal plasma 137 +/- 75.2 mug/g d.w. (see figs. 1 and 2). One of the Dag defect's main localizations, the outer dense tail fibers, has a very high zinc content and this fact together with the heredity of the defect make further studies most promising.", "contents": "Zinc as a possible causal factor in the sterilizing sperm tail defect, the 'Dag-defect', in Jersey bulls. The zinc content was determined in semen samples from four young Danish Jersey bulls showing the typical 'Dag defect' (greater than 50% strongly coiled or bent sperm tails). Two of the bulls were half brothers. Semen samples from 14 normal fertile Jersey bulls served as controls. The normal material showed a zinc content in the centrifuged sperm of 190 +/- 15.8 mug/g d.w. and in the seminal plasma 81 +/- 31.2 mug/g d.w. In the four abnormal bulls the following elevated values were found: in the centrifuged sperm 310 +/- 109.5 mug/g d.w. and in the seminal plasma 137 +/- 75.2 mug/g d.w. (see figs. 1 and 2). One of the Dag defect's main localizations, the outer dense tail fibers, has a very high zinc content and this fact together with the heredity of the defect make further studies most promising.", "PMID": 980695}
{"id": "pubmed23n0032_12499", "title": "Epidemiology of mycotoxic porcine nephropathy.", "content": "Mycotoxic porcine nephropathy is a renal disorder caused by ingestion of feed-borne secondary fungal metabolites, possessing nephrotoxic properties. The disease is present endemically in all areas of Denmark, but unevenly distributed, with frequency varying from 0.6 to 65.9 cases per 10,000 pigs in 1971. Epidemics were encountered in 1963 and 1971, apparently associated with excessive climatic conditions. The highest frequency of the disease is found among pigs from the smaller farms. Ordinarily the same farm delivers cases during only one year. Female pigs contract the disease more frequently than male (castrated) pigs. The epidemiology of mycotoxic porcine nephropathy shows similarities with the epidemiology of endemic Balkan nephropathy.", "contents": "Epidemiology of mycotoxic porcine nephropathy. Mycotoxic porcine nephropathy is a renal disorder caused by ingestion of feed-borne secondary fungal metabolites, possessing nephrotoxic properties. The disease is present endemically in all areas of Denmark, but unevenly distributed, with frequency varying from 0.6 to 65.9 cases per 10,000 pigs in 1971. Epidemics were encountered in 1963 and 1971, apparently associated with excessive climatic conditions. The highest frequency of the disease is found among pigs from the smaller farms. Ordinarily the same farm delivers cases during only one year. Female pigs contract the disease more frequently than male (castrated) pigs. The epidemiology of mycotoxic porcine nephropathy shows similarities with the epidemiology of endemic Balkan nephropathy.", "PMID": 980696}
{"id": "pubmed23n0032_12500", "title": "A study of skin diseases in dogs and cats. II. Microflora of the normal skin of dogs and cats.", "content": "A study of the microflora of the normal skin was undertaken in 10 dogs and 10 cats. Swabs were taken from the surface of the skin at 7 different sites (Fig. 1). Micrococcus spp., alpha-hemolytic streptococci, and Acinetobacter spp. were found consistently in both species. Staphylococcus aureus was isolated from 9 dogs and 4 cats, and staphylococcus epidermidis from 7 dogs and 5 cats. Beta-hemolytic streptococci, Corynebacterium spp., Bacillus spp., Escherichia coli, Proteus mirabilis, Pseudomonas spp., and Alcaligenes spp. were found sporadically.", "contents": "A study of skin diseases in dogs and cats. II. Microflora of the normal skin of dogs and cats. A study of the microflora of the normal skin was undertaken in 10 dogs and 10 cats. Swabs were taken from the surface of the skin at 7 different sites (Fig. 1). Micrococcus spp., alpha-hemolytic streptococci, and Acinetobacter spp. were found consistently in both species. Staphylococcus aureus was isolated from 9 dogs and 4 cats, and staphylococcus epidermidis from 7 dogs and 5 cats. Beta-hemolytic streptococci, Corynebacterium spp., Bacillus spp., Escherichia coli, Proteus mirabilis, Pseudomonas spp., and Alcaligenes spp. were found sporadically.", "PMID": 980697}
{"id": "pubmed23n0032_12501", "title": "[Isotope study of the effects of a nitrate derivative on pulmonary blood volume. Application in the treatment of cardiogenic pulmonary edema].", "content": "Isotopic study of pulmonary and ventricular blood volumes after the ingestion of 5 mg of isosorbide dinitrate showed that cardio-pulmonary blood volume decreased by 380 ml without any change in total circulating blood volume. The demonstration of this effective \"internal venesection\" explains the fall in capillary pressure seen after the administration of nitrate derivatives. It justifies their use in the treatment of acute pulmonary oedema or refractory cardiac failure and explains the results observed.", "contents": "[Isotope study of the effects of a nitrate derivative on pulmonary blood volume. Application in the treatment of cardiogenic pulmonary edema]. Isotopic study of pulmonary and ventricular blood volumes after the ingestion of 5 mg of isosorbide dinitrate showed that cardio-pulmonary blood volume decreased by 380 ml without any change in total circulating blood volume. The demonstration of this effective \"internal venesection\" explains the fall in capillary pressure seen after the administration of nitrate derivatives. It justifies their use in the treatment of acute pulmonary oedema or refractory cardiac failure and explains the results observed.", "PMID": 980711}
{"id": "pubmed23n0032_12502", "title": "[Severe hyperkalemia in cardiac patients].", "content": "Hyperkalaemia with severe myocardial consequence may complicate the treatment of heart failure. In five patients who developed kalaemia ranging from 6.5. to 8.6. mEq/l, the ECG showed altered auriculogram and/or widened QRS, the latter change being associated with ST segment elevation and very large T wave in one case. Such hyperkalaemia cannot be termed iatrogenic, although it is promoted by diuretic-induced hyponatraemia and dehydration. The essential part is played by an aggravation of the haemodynamic status, responsible for acute renal insufficiency with oligoanuria, and by the attendant metabolic acidosis. The correction of this metabolic acidosis promotes diuresis, causing potassium depletion and the rapid regression of electrocardiographic abnormalities. Parenteral alkalinization with sodium bicarbonate, associated with furosemid to prevent sodium overload, instituted in emergency, is the only way to prevent asystole or ventricular fibrillation when kalaemia exceeds 8 mEq/l.", "contents": "[Severe hyperkalemia in cardiac patients]. Hyperkalaemia with severe myocardial consequence may complicate the treatment of heart failure. In five patients who developed kalaemia ranging from 6.5. to 8.6. mEq/l, the ECG showed altered auriculogram and/or widened QRS, the latter change being associated with ST segment elevation and very large T wave in one case. Such hyperkalaemia cannot be termed iatrogenic, although it is promoted by diuretic-induced hyponatraemia and dehydration. The essential part is played by an aggravation of the haemodynamic status, responsible for acute renal insufficiency with oligoanuria, and by the attendant metabolic acidosis. The correction of this metabolic acidosis promotes diuresis, causing potassium depletion and the rapid regression of electrocardiographic abnormalities. Parenteral alkalinization with sodium bicarbonate, associated with furosemid to prevent sodium overload, instituted in emergency, is the only way to prevent asystole or ventricular fibrillation when kalaemia exceeds 8 mEq/l.", "PMID": 980725}
{"id": "pubmed23n0032_12503", "title": "[Effect of a polyvitamin preparation on the immune response to antibodies. Demonstration of the immunostimulating activity of propylene glycol].", "content": "Humoral antibody to sheep erythrocytes was studied by plaque forming cell and hemagglutinin assays in mice receiving a polyvitaminic preparation containing vitamins A, D, E, and C. A statistically significant increase in PFC on days 6, 12 and 18 after immunization, and an increase in hemagglutinin titers was observed in animals treated with this vitamin containing solution. These results are interpreted as an immunostimulating activity of the preparation. Nevertheless, as shown by the results obtained with each component injected separately, a major proportion of the immunostimulating activity seems to be related to propylene glycol, a simple compound contained as an hydrodispersant in the preparation. Further studies of the immunostimulating properties of propylene glycol are now in progress in our laboratory.", "contents": "[Effect of a polyvitamin preparation on the immune response to antibodies. Demonstration of the immunostimulating activity of propylene glycol]. Humoral antibody to sheep erythrocytes was studied by plaque forming cell and hemagglutinin assays in mice receiving a polyvitaminic preparation containing vitamins A, D, E, and C. A statistically significant increase in PFC on days 6, 12 and 18 after immunization, and an increase in hemagglutinin titers was observed in animals treated with this vitamin containing solution. These results are interpreted as an immunostimulating activity of the preparation. Nevertheless, as shown by the results obtained with each component injected separately, a major proportion of the immunostimulating activity seems to be related to propylene glycol, a simple compound contained as an hydrodispersant in the preparation. Further studies of the immunostimulating properties of propylene glycol are now in progress in our laboratory.", "PMID": 980726}
{"id": "pubmed23n0032_12504", "title": "[Testicular secretions of androgens after prolonged physical effort in man].", "content": "Variations in plasma testosterone, dihydrotestosterone. delta 4 androstenedione, LH, FSH and cortisol were measured before and after 6 succesive effort tests in 11 male subjects. Testicular secretion and the extra-hepatic consumption of androgens are increased by the haemodynamic conditions of effort but plasma levels of testosterone and dihydrotestosterone fall with repeated effort, indicating a progressive imbalance between production and consumption. These variations suggest that the testis, rather than glucocorticoid function of the adrenal, might play a major limiting role, psychological and metabolic, in tolerance of prolonged effort.", "contents": "[Testicular secretions of androgens after prolonged physical effort in man]. Variations in plasma testosterone, dihydrotestosterone. delta 4 androstenedione, LH, FSH and cortisol were measured before and after 6 succesive effort tests in 11 male subjects. Testicular secretion and the extra-hepatic consumption of androgens are increased by the haemodynamic conditions of effort but plasma levels of testosterone and dihydrotestosterone fall with repeated effort, indicating a progressive imbalance between production and consumption. These variations suggest that the testis, rather than glucocorticoid function of the adrenal, might play a major limiting role, psychological and metabolic, in tolerance of prolonged effort.", "PMID": 980728}
{"id": "pubmed23n0032_12505", "title": "[Water consumption in Saharan nomads. A remarkably reduced and constant consumption].", "content": "The writer has carried out two studies of the Western Sahara (Missions UNESCO-Institut Scientifique Cherifien 1961 and 1964) bearing upon the consumption of water by the Saharan nomads. In spite of their environment, the consumption appeared to be remarkably low and constant. Very strict dietary practices, the salinity of the water, and special behavioural customs seemed to be the basis of this strict economy of fluid intake. Genetic factors could also, of course, be partly responsible. The dietary practices are characterised essentially: 1) by a regime which in general contains the minimum of protein: milk foods, cereals, and sugars, and 2) by the habitual exclusion of salt in the preparation of meals; the sodium necessary for water/sodium balance deriving solely from the salinity of the water. Water with little or average salt content (total 2-3 g/l) seems to meet the normal needs of the body - there is no need for the salt pill - and, moreover, to quench the thirst more effectively than pure water (Paque, 1964) - presumably by making good the deficit (cf. Stricker, 1970). On the other hand, saltier water (total 4-8g/; Na 1 g/l or more) appears to pose more complex physiological problems for which the prime solution is to apply the Saharan rule, i.e. that no supplementary salt should be added to the diet (Paque, 1963). In desert life there are thus certain rules which must be obeyed. As for the matter of behavioural customs influencing water balance, they consist in the main of habitually limiting the frequency of water intake (often just twice daily, sometime only once daily) together with a careful choice of clothing and the wearing of the veil. Finally, genetic factor could result in a more efficiently controlled loss of water (and of salt?) VIA THE SKIN.", "contents": "[Water consumption in Saharan nomads. A remarkably reduced and constant consumption]. The writer has carried out two studies of the Western Sahara (Missions UNESCO-Institut Scientifique Cherifien 1961 and 1964) bearing upon the consumption of water by the Saharan nomads. In spite of their environment, the consumption appeared to be remarkably low and constant. Very strict dietary practices, the salinity of the water, and special behavioural customs seemed to be the basis of this strict economy of fluid intake. Genetic factors could also, of course, be partly responsible. The dietary practices are characterised essentially: 1) by a regime which in general contains the minimum of protein: milk foods, cereals, and sugars, and 2) by the habitual exclusion of salt in the preparation of meals; the sodium necessary for water/sodium balance deriving solely from the salinity of the water. Water with little or average salt content (total 2-3 g/l) seems to meet the normal needs of the body - there is no need for the salt pill - and, moreover, to quench the thirst more effectively than pure water (Paque, 1964) - presumably by making good the deficit (cf. Stricker, 1970). On the other hand, saltier water (total 4-8g/; Na 1 g/l or more) appears to pose more complex physiological problems for which the prime solution is to apply the Saharan rule, i.e. that no supplementary salt should be added to the diet (Paque, 1963). In desert life there are thus certain rules which must be obeyed. As for the matter of behavioural customs influencing water balance, they consist in the main of habitually limiting the frequency of water intake (often just twice daily, sometime only once daily) together with a careful choice of clothing and the wearing of the veil. Finally, genetic factor could result in a more efficiently controlled loss of water (and of salt?) VIA THE SKIN.", "PMID": 980741}
{"id": "pubmed23n0032_12506", "title": "[Treatment of type II familial hypercholesterolemia by portacaval anastomosis].", "content": "Termino-lateral porto-caval anastomosis seems at present to be the most effective treatment for severe familial hypercholesterolaemia. The mechanism by which it acts has not been determined and its long term results are not known. One success and one failure confirm the immediate action of anastomosis in a condition with a fatal prognosis.", "contents": "[Treatment of type II familial hypercholesterolemia by portacaval anastomosis]. Termino-lateral porto-caval anastomosis seems at present to be the most effective treatment for severe familial hypercholesterolaemia. The mechanism by which it acts has not been determined and its long term results are not known. One success and one failure confirm the immediate action of anastomosis in a condition with a fatal prognosis.", "PMID": 980742}
{"id": "pubmed23n0032_12507", "title": "[Myocardial and coronary lesions in anterior and posterior myocardial infarction. Comparative macroscopic anatomical study].", "content": "Comparative anatomical macroscopic study of two groups of 24 cases of recently fatal first myocardial infarction (anterior or inferior) confirmed certain classical notions: higher age in females (p less than 0.02), inferior necrosis less extensive (p less than 0.05), higher occurrence of rupture of the myocardium in the case or an anterior lesion, and less frequent rupture but more often involving the septum in the case of inferior infarction. Furthermore the study showed the virtually constant presence of an intracoronary thrombus responsible for the infarction, the wider extent of atherosclerotic lesions in the right coronary system, and, above all, a fairly direct opposition between stenotic lesions most often proximal, and affecting the single anterior trunk (75%), in the case of anterior infarction, whilst significant atherosclerotic occlusion of the anterior (87%) and left (67%) systems were seen in the case of inferior infarction due to complete occlusion of the right coronary artery. These anatomical findings indicate the need for routine coronary arteriography after inferior infarctions, the recurrence of chest pain being related not to changes \"in sity\" but rather to significant occlusion of another coronary system.", "contents": "[Myocardial and coronary lesions in anterior and posterior myocardial infarction. Comparative macroscopic anatomical study]. Comparative anatomical macroscopic study of two groups of 24 cases of recently fatal first myocardial infarction (anterior or inferior) confirmed certain classical notions: higher age in females (p less than 0.02), inferior necrosis less extensive (p less than 0.05), higher occurrence of rupture of the myocardium in the case or an anterior lesion, and less frequent rupture but more often involving the septum in the case of inferior infarction. Furthermore the study showed the virtually constant presence of an intracoronary thrombus responsible for the infarction, the wider extent of atherosclerotic lesions in the right coronary system, and, above all, a fairly direct opposition between stenotic lesions most often proximal, and affecting the single anterior trunk (75%), in the case of anterior infarction, whilst significant atherosclerotic occlusion of the anterior (87%) and left (67%) systems were seen in the case of inferior infarction due to complete occlusion of the right coronary artery. These anatomical findings indicate the need for routine coronary arteriography after inferior infarctions, the recurrence of chest pain being related not to changes \"in sity\" but rather to significant occlusion of another coronary system.", "PMID": 980743}
{"id": "pubmed23n0032_12508", "title": "[Immunologic evaluations during prolonged and chronic hepatitis. Significance for diagnosis and prognosis. 100 cases].", "content": "This retrospective study illustrates the valuable contribution of immunological studies carried out during the third month of hepatitis. The persistence of HBs antigen at this stage of the disease considerably increases the risk of chronic hepatitis though not necessarily implying progression to cirrhosis. The latter should be feared if there is a marked increase in gamma globulins and in the presence of a high level of anti-smooth muscle and antinuclear antibodies. Amongst the other immunological features, an increase in IgA and IgM also indicates the possibility of progression from chronic hepatitis to cirrhosis. Comparative study in chronic active hepatitis of HBs antigen and various antibodies indicates essentially three types: - CAH with Ag HBs without auto-antibodies - Auto-immune CAH without Ag HBs - CAH without auto-antibodies nor Ag HBs. Progression to cirrhosis appears to be less frequent in the first group than in the second.", "contents": "[Immunologic evaluations during prolonged and chronic hepatitis. Significance for diagnosis and prognosis. 100 cases]. This retrospective study illustrates the valuable contribution of immunological studies carried out during the third month of hepatitis. The persistence of HBs antigen at this stage of the disease considerably increases the risk of chronic hepatitis though not necessarily implying progression to cirrhosis. The latter should be feared if there is a marked increase in gamma globulins and in the presence of a high level of anti-smooth muscle and antinuclear antibodies. Amongst the other immunological features, an increase in IgA and IgM also indicates the possibility of progression from chronic hepatitis to cirrhosis. Comparative study in chronic active hepatitis of HBs antigen and various antibodies indicates essentially three types: - CAH with Ag HBs without auto-antibodies - Auto-immune CAH without Ag HBs - CAH without auto-antibodies nor Ag HBs. Progression to cirrhosis appears to be less frequent in the first group than in the second.", "PMID": 980757}
{"id": "pubmed23n0032_12509", "title": "[Does chronic psychosis due to amphetamines abuse exist? Study of 25 drug addicts].", "content": "The development of a psychotic process was noted in seven amphetamine addicts who had been seen over a period of several years before onset of the psychosis and who had no psychotic tendencies before the addiction. This group was compared with two from the standpoint of the symptomatology of acute psychosis; i.e. acute psychotic episodes under the effects of amphetamines in individuals who were not psychotic before addiction and who were cured at present, and acute psychotic episodes under the effects of amphetamines in individuals who were psychotic at the time of becoming amphetamine addicts. This comparative study attempts to define the signs which precede the development of a chronic psychosis and includes a discussion concerning the clinical picture and its correlation with the pre-existent personality.", "contents": "[Does chronic psychosis due to amphetamines abuse exist? Study of 25 drug addicts]. The development of a psychotic process was noted in seven amphetamine addicts who had been seen over a period of several years before onset of the psychosis and who had no psychotic tendencies before the addiction. This group was compared with two from the standpoint of the symptomatology of acute psychosis; i.e. acute psychotic episodes under the effects of amphetamines in individuals who were not psychotic before addiction and who were cured at present, and acute psychotic episodes under the effects of amphetamines in individuals who were psychotic at the time of becoming amphetamine addicts. This comparative study attempts to define the signs which precede the development of a chronic psychosis and includes a discussion concerning the clinical picture and its correlation with the pre-existent personality.", "PMID": 980758}
{"id": "pubmed23n0032_12510", "title": "[Cervical ribs. Therapeutic deliberations apropos of 10 cases].", "content": "The authors report ten cases of cervical ribs operated upon in 7 patients. The predominantly neurological symptoms and signs were associated with vascular problems in 3 cases, with intermittent compression of the sub-clavian vessels. Treatment consisted of resection of the cervical rib and the first rib via an extra-pleural axillary approach using the technique described by Roos. It gave 10 good results with a follow-up of 6 months to 3 years. The authors emphasise the fact that this pathology falls within the context of compressive syndromes of the root of the upper limb or thoracic outlet syndrome. Such a conception justifies associated resection of the first rib which should give better long term results than simple resection of the extra rib.", "contents": "[Cervical ribs. Therapeutic deliberations apropos of 10 cases]. The authors report ten cases of cervical ribs operated upon in 7 patients. The predominantly neurological symptoms and signs were associated with vascular problems in 3 cases, with intermittent compression of the sub-clavian vessels. Treatment consisted of resection of the cervical rib and the first rib via an extra-pleural axillary approach using the technique described by Roos. It gave 10 good results with a follow-up of 6 months to 3 years. The authors emphasise the fact that this pathology falls within the context of compressive syndromes of the root of the upper limb or thoracic outlet syndrome. Such a conception justifies associated resection of the first rib which should give better long term results than simple resection of the extra rib.", "PMID": 980759}
{"id": "pubmed23n0032_12511", "title": "[Disorders of hemostasis during partial hepatectomies. Study of 20 cases].", "content": "Changes in blood coagulation have been studied during and after 20 elective hepatic lobectomies, most of them for tumors necessitating extensive resection (17 right lobectomies, 10 of which were extended to the medial segment of the left lobe). In addition, 9 unresectable tumors, as proved by exploratory laparotomy, were taken for controls. Before operation, hemostasis in essentially normal except a hyperfibrinogenemia and a slight increase of fibrinogen split products in half the cases. Factor VIII antigen levels are consistently increased. During mobilization of the liver an increased fibrinolytic activity and soluble complexes are frequently observed. Fibrinolysic activity spontaneously returns to normal at the end of the operation, except in cirrhotic patients. No bleeding disthesis is observed and haemorrhage appears to be related to technical problems. On the other hand, there is no clinical evidence of thromboembolism after operation. Therefore any specific therapy either pro or anti-coagulant, appears unjustified, apart from the eventual and transient administration of antifibrinolytic drugs in cirrhotic patients. In the post operative period, blood clotting factors synthetized by the liver (prothrombin complex and antithrombin III) slowly return to normal. Rapid correction of hypo-accelerinaemia reflects the onset of liver regeneration. A simplified method for surveying hemostasis during and after liver resection is proposed.", "contents": "[Disorders of hemostasis during partial hepatectomies. Study of 20 cases]. Changes in blood coagulation have been studied during and after 20 elective hepatic lobectomies, most of them for tumors necessitating extensive resection (17 right lobectomies, 10 of which were extended to the medial segment of the left lobe). In addition, 9 unresectable tumors, as proved by exploratory laparotomy, were taken for controls. Before operation, hemostasis in essentially normal except a hyperfibrinogenemia and a slight increase of fibrinogen split products in half the cases. Factor VIII antigen levels are consistently increased. During mobilization of the liver an increased fibrinolytic activity and soluble complexes are frequently observed. Fibrinolysic activity spontaneously returns to normal at the end of the operation, except in cirrhotic patients. No bleeding disthesis is observed and haemorrhage appears to be related to technical problems. On the other hand, there is no clinical evidence of thromboembolism after operation. Therefore any specific therapy either pro or anti-coagulant, appears unjustified, apart from the eventual and transient administration of antifibrinolytic drugs in cirrhotic patients. In the post operative period, blood clotting factors synthetized by the liver (prothrombin complex and antithrombin III) slowly return to normal. Rapid correction of hypo-accelerinaemia reflects the onset of liver regeneration. A simplified method for surveying hemostasis during and after liver resection is proposed.", "PMID": 980762}
{"id": "pubmed23n0032_12512", "title": "[Hepatic puncture biopsy by the transjugular route].", "content": "The authors report their experience of transjugular liver biopsy, a method which consists in taking a liver specimen with a needle placed in a hepatic vein. This method was used in 13 patients in whom conventional liver needle biopsy was contraindicated because of massive ascites or bleeding tendency. In 10 of these patients, a liver specimen was obtained; in patients with cirrhosis or chronic active hepatitis, the liver specimens were more or less divided into small fragments, which prevented correct assessment of liver architecture in three of them. The examination was well tolerated. The authors conclude that transjugular liver biopsy is not technically difficult in centers where hepatic vein catheterization is available and that this method is indicated in patients in whom conventional liver needle biopsy is contraindicated.", "contents": "[Hepatic puncture biopsy by the transjugular route]. The authors report their experience of transjugular liver biopsy, a method which consists in taking a liver specimen with a needle placed in a hepatic vein. This method was used in 13 patients in whom conventional liver needle biopsy was contraindicated because of massive ascites or bleeding tendency. In 10 of these patients, a liver specimen was obtained; in patients with cirrhosis or chronic active hepatitis, the liver specimens were more or less divided into small fragments, which prevented correct assessment of liver architecture in three of them. The examination was well tolerated. The authors conclude that transjugular liver biopsy is not technically difficult in centers where hepatic vein catheterization is available and that this method is indicated in patients in whom conventional liver needle biopsy is contraindicated.", "PMID": 980772}
{"id": "pubmed23n0032_12513", "title": "[Pregnancy in women with atrioventricular block. 13 cases].", "content": "Thirteen women aged between 18 and 37 years and suffering from atrio-ventricular block had 36 pregnancies. Foetal prognosis was excellent and the pregnancy quite unaffected by the block in 9 cases. In 4 women, Stokes-Adams attacks occurred. In one case from some time ago therapeutic abortion was necessary. Temporary pacing at the time of delivery was used in one case and a permanent pacemaker in one patient. The problems posed by the insertion of a pacemaker in a woman of childbearing age are discussed.", "contents": "[Pregnancy in women with atrioventricular block. 13 cases]. Thirteen women aged between 18 and 37 years and suffering from atrio-ventricular block had 36 pregnancies. Foetal prognosis was excellent and the pregnancy quite unaffected by the block in 9 cases. In 4 women, Stokes-Adams attacks occurred. In one case from some time ago therapeutic abortion was necessary. Temporary pacing at the time of delivery was used in one case and a permanent pacemaker in one patient. The problems posed by the insertion of a pacemaker in a woman of childbearing age are discussed.", "PMID": 980773}
{"id": "pubmed23n0032_12514", "title": "[Combination chemotherapy in the treatment of polymetastic breast cancer. Comparison of therapeutic effects of 2 methods of sequential drug administration. Role of adriamycin in these combinations].", "content": "One thousand and twenty four patients with disseminated breast cancer were submitted to combination chemotherapy. Fifty one patients (group I) were sequentially given VCR, CPM and 5 FU, and seventy three patients (group II) were given ADM, VCR, CPM and 5 FU. The general and haematological tolerance was good and comparable in the both groups of patients: we observed only two severe infectious complications. Bonemarrow hypoplasia, six myocardial ischemia (two of them were lethal) in each group of patients, without any predominance in the group of patients treated with adriamycin. The percentage of objective regression in both groups was respectively: 72% and 71%. The mean duration of response was eight months. The median survival time was 420 days for patients of group I; for patients of group II the median is not obtained at 480 days. This study confirms that responders to chemotherapy significantly increase the mean duration of survival time. However, in this group of responders the presence of the liver metastases is worse prognosis than all other visceral metastases.", "contents": "[Combination chemotherapy in the treatment of polymetastic breast cancer. Comparison of therapeutic effects of 2 methods of sequential drug administration. Role of adriamycin in these combinations]. One thousand and twenty four patients with disseminated breast cancer were submitted to combination chemotherapy. Fifty one patients (group I) were sequentially given VCR, CPM and 5 FU, and seventy three patients (group II) were given ADM, VCR, CPM and 5 FU. The general and haematological tolerance was good and comparable in the both groups of patients: we observed only two severe infectious complications. Bonemarrow hypoplasia, six myocardial ischemia (two of them were lethal) in each group of patients, without any predominance in the group of patients treated with adriamycin. The percentage of objective regression in both groups was respectively: 72% and 71%. The mean duration of response was eight months. The median survival time was 420 days for patients of group I; for patients of group II the median is not obtained at 480 days. This study confirms that responders to chemotherapy significantly increase the mean duration of survival time. However, in this group of responders the presence of the liver metastases is worse prognosis than all other visceral metastases.", "PMID": 980774}
{"id": "pubmed23n0032_12515", "title": "[Value of radionuclide imaging for the emergency diagnosis of pulmonary embolism. 27 cases].", "content": "The urgent nature of certain cases of pulmonary ambolism limits the examinations possible to a minimum which should be rapid, immediately interpretable, absolutley harmless and of proven reliability. We reviewed 27 cases of patients referred fro possible embolectomy in a cardiovascular surgery service from 1968 to 1975. Infusion scintigraphy was carried out in all the patients and 20 underwent surgery. Retrospective study of these cases indicates that infusion lung scintigraphy confirmed the diagnosis in all 27 and indicated the need for medical or surgical treatment. The problems which were posed by certain scintigraphic images obtained using a scanning apparatus with iodine mabelled macro-agregates have disappeared with current techniques: the use of microemboli labelled with Technetium, a short-life low energy isotope, together with a scintillation camera which gives rapid AP and lateral views, the latter being essential in the determination of the topographical distribution of the defects.", "contents": "[Value of radionuclide imaging for the emergency diagnosis of pulmonary embolism. 27 cases]. The urgent nature of certain cases of pulmonary ambolism limits the examinations possible to a minimum which should be rapid, immediately interpretable, absolutley harmless and of proven reliability. We reviewed 27 cases of patients referred fro possible embolectomy in a cardiovascular surgery service from 1968 to 1975. Infusion scintigraphy was carried out in all the patients and 20 underwent surgery. Retrospective study of these cases indicates that infusion lung scintigraphy confirmed the diagnosis in all 27 and indicated the need for medical or surgical treatment. The problems which were posed by certain scintigraphic images obtained using a scanning apparatus with iodine mabelled macro-agregates have disappeared with current techniques: the use of microemboli labelled with Technetium, a short-life low energy isotope, together with a scintillation camera which gives rapid AP and lateral views, the latter being essential in the determination of the topographical distribution of the defects.", "PMID": 980788}
{"id": "pubmed23n0032_12516", "title": "[The small left colon syndrome. 3 cases].", "content": "In the light of three cases of neonatal obstruction related to a small calibre descending colon, the authors review the main clinical and radiological signs and compare them with the data in the literature. This functional disturbance is related to immaturity of the intrinsic innervation of the colon which is especially common in low birth weight neonates or of diabetic mothers. Diagnosis is dependent upon enema using diluted uroangiographic hydrosoluble iodine contrast medium, which shows a disparity in calibre at the left colic angle. The differential diagnosis lies with Hirschsprung's disease. Functional ileus in premature infants, meconium ileus and the \"small left colon syndrome\" are related to the same pathogenisis: functional immaturity of the colon.", "contents": "[The small left colon syndrome. 3 cases]. In the light of three cases of neonatal obstruction related to a small calibre descending colon, the authors review the main clinical and radiological signs and compare them with the data in the literature. This functional disturbance is related to immaturity of the intrinsic innervation of the colon which is especially common in low birth weight neonates or of diabetic mothers. Diagnosis is dependent upon enema using diluted uroangiographic hydrosoluble iodine contrast medium, which shows a disparity in calibre at the left colic angle. The differential diagnosis lies with Hirschsprung's disease. Functional ileus in premature infants, meconium ileus and the \"small left colon syndrome\" are related to the same pathogenisis: functional immaturity of the colon.", "PMID": 980789}
{"id": "pubmed23n0032_12517", "title": "Diagnosis of superior vena caval obstruction without syndrome.", "content": "Ten patients with known malignant intra-thoracic tumors and no evidence of superior vena cava syndrome were evaluated by means of scintillation camera superior venacavograms. Four of the ten patients proved to have unsuspected involvement of the superior vena cava or its tributaries. The scintillation camer superior venacavogram provides a quick, safe, and accurate method of evaluating the patency of the SVC and its tributaries. We suggest that it should be routinely performed on all patients prior to beginning radiation therapy for malignant intra-thoracic tumors.", "contents": "Diagnosis of superior vena caval obstruction without syndrome. Ten patients with known malignant intra-thoracic tumors and no evidence of superior vena cava syndrome were evaluated by means of scintillation camera superior venacavograms. Four of the ten patients proved to have unsuspected involvement of the superior vena cava or its tributaries. The scintillation camer superior venacavogram provides a quick, safe, and accurate method of evaluating the patency of the SVC and its tributaries. We suggest that it should be routinely performed on all patients prior to beginning radiation therapy for malignant intra-thoracic tumors.", "PMID": 980792}
{"id": "pubmed23n0032_12518", "title": "[Changes in perfusion and blood flow distribution following normo- and hypothermic ischemia of the kidneys].", "content": "Experimental examinations were performed in 22 dogs to find out the mechanism which leads to a permanent or a reversible damage of the renal parenchyma after normo- and hypothermic ischemia. For this reason the perfusion and the distribution were examined with 133Xe, the vascular changes by angiography, and the parenchymal function with 131I-Hippuran. After normothermic ischemia a short-term reactive hyperemia appeared, which however could not compensate the damage of the renal tubular cells and the resulting excretory insufficiency. After hypothermic ischemia the perfusion was reduced, probably as a consequence of a vasconstriction by cold, however, the function of the tubular cells remained intact, because of the protective mechanism of the hypothermia. The importance of these findings for the development of the so-called \"shock-kidney\" (acute tubular necrosis) and for the conservative renal surgery in hypothermia is discussed and the application of measures beneficial to perfusion, are suggested.", "contents": "[Changes in perfusion and blood flow distribution following normo- and hypothermic ischemia of the kidneys]. Experimental examinations were performed in 22 dogs to find out the mechanism which leads to a permanent or a reversible damage of the renal parenchyma after normo- and hypothermic ischemia. For this reason the perfusion and the distribution were examined with 133Xe, the vascular changes by angiography, and the parenchymal function with 131I-Hippuran. After normothermic ischemia a short-term reactive hyperemia appeared, which however could not compensate the damage of the renal tubular cells and the resulting excretory insufficiency. After hypothermic ischemia the perfusion was reduced, probably as a consequence of a vasconstriction by cold, however, the function of the tubular cells remained intact, because of the protective mechanism of the hypothermia. The importance of these findings for the development of the so-called \"shock-kidney\" (acute tubular necrosis) and for the conservative renal surgery in hypothermia is discussed and the application of measures beneficial to perfusion, are suggested.", "PMID": 980793}
{"id": "pubmed23n0032_12519", "title": "Determination of placental blood flow by external monitoring of 113In.", "content": "A simple and noninvasive technique was developed to evaluate the blood perfusion rate within human placenta. The method is based on monitoring the accumulation of the isotope 113mIn in the placental intervillous space using a single detector as a measuring device. The result was expressed as an accumulation index calculated from the tracer appearance curve. The In accumulation index was significantly lower in preeclamptic patients than in the controls, but with a marked overlapping. The In index was significantly correlated with the simultaneously recorded maternal minute volume, 24-hour estrogen excretion and birthweight. No correlation was found with the placental weight or Laakso's perfusion index. Uterine contractions induced a significant decrease in the In index. The authors conclude that the In accumulation index can be used as an objective measure of the placental blood flow.", "contents": "Determination of placental blood flow by external monitoring of 113In. A simple and noninvasive technique was developed to evaluate the blood perfusion rate within human placenta. The method is based on monitoring the accumulation of the isotope 113mIn in the placental intervillous space using a single detector as a measuring device. The result was expressed as an accumulation index calculated from the tracer appearance curve. The In accumulation index was significantly lower in preeclamptic patients than in the controls, but with a marked overlapping. The In index was significantly correlated with the simultaneously recorded maternal minute volume, 24-hour estrogen excretion and birthweight. No correlation was found with the placental weight or Laakso's perfusion index. Uterine contractions induced a significant decrease in the In index. The authors conclude that the In accumulation index can be used as an objective measure of the placental blood flow.", "PMID": 980794}
{"id": "pubmed23n0032_12520", "title": "Characterization of an intravenously injected bolus.", "content": "A study of some parameters affecting the time activity histogram of an intravenous bolus injection of radioactivity was performed. A scoring system for bolus compactness was attempted. A score of 2 and above was considered to be a satisfactory bolus. Volumes less than 1 ml tended to result in a satisfactory bolus. The nature of the radiopharmaceutical injected, different injecters and age of the patient did not affect the score. Thyrotoxic patients gave the best bolus score.", "contents": "Characterization of an intravenously injected bolus. A study of some parameters affecting the time activity histogram of an intravenous bolus injection of radioactivity was performed. A scoring system for bolus compactness was attempted. A score of 2 and above was considered to be a satisfactory bolus. Volumes less than 1 ml tended to result in a satisfactory bolus. The nature of the radiopharmaceutical injected, different injecters and age of the patient did not affect the score. Thyrotoxic patients gave the best bolus score.", "PMID": 980795}
{"id": "pubmed23n0032_12521", "title": "57Co-hematoporphyrin accumulation by experimental tumors.", "content": "The in vivo distribution of 57Co-hematoporphyrin in adenocarcinoma BW10232-bearing mice has been studied. Tumor-bearing and normal animals exhibit similar patterns of radioactivity accumulation. Twenty-four hours after the administration of the radiocompound the ratios tumor to blood and tumor to muscle indicate a potential value of this radioactive porphyrin for the detection of some types of tumor.", "contents": "57Co-hematoporphyrin accumulation by experimental tumors. The in vivo distribution of 57Co-hematoporphyrin in adenocarcinoma BW10232-bearing mice has been studied. Tumor-bearing and normal animals exhibit similar patterns of radioactivity accumulation. Twenty-four hours after the administration of the radiocompound the ratios tumor to blood and tumor to muscle indicate a potential value of this radioactive porphyrin for the detection of some types of tumor.", "PMID": 980796}
{"id": "pubmed23n0032_12522", "title": "Mechanism of 67Ga accumulation in normal rat liver lysosomes.", "content": "67Ga accumulates in various malignant tumors and parenchymatous tissues. It was found to be associated with the soluble fraction of lysosomes (11). The present work investigates the mechanism of 67Ga accumulation in normal liver cells. Lysosomes were isolated from rat liver after intravenous injection of carrier free 67Ga. The soluble lysosomal fraction was obtained by sonication followed by centrifugation at 105,000 xg for 2 hrs. Gel filtration on Sephadex G 25 superfine was carried out on the soluble lysosomal fraction in order to investigate the stability of the 67Ga-protein complex within the lysosomes under EDTA treatment. After treatment with 1 mM/l EDTA a considerable amount of the protein bound radioactivity was found to be liberated. In further experiments the 67Ga binding lysosomal proteins were fractionated by electrophoresis on 7% polyacrylamide gels (0.5 cm x 5.5 cm). After staining with Coomassie blue 18 separated protein bands were apparent. 67Ga distribution within the gels was assessed by direct counting of radioactivity in gel slices. A considerable amount of the intralysosomal protein bound radioactivity migrated with a relative mobility of 0.36 corresponding to a protein band of molecular weight 85,000--90,000. This peak corresponded to the peak of 67Ga-labelled purified transferrin in control gels. These data were confirmed by immunoelectrophoresis combined with autoradiography: within the soluble lysosomal fraction a slight transferrin line could be identified. We conclude that 67Ga which is transported in the blood by transferrin (23) and taken up by the hepatic cell through endocytosis (32) is accumulated in the lysosomes associated with transferrin and its degraded fragments.", "contents": "Mechanism of 67Ga accumulation in normal rat liver lysosomes. 67Ga accumulates in various malignant tumors and parenchymatous tissues. It was found to be associated with the soluble fraction of lysosomes (11). The present work investigates the mechanism of 67Ga accumulation in normal liver cells. Lysosomes were isolated from rat liver after intravenous injection of carrier free 67Ga. The soluble lysosomal fraction was obtained by sonication followed by centrifugation at 105,000 xg for 2 hrs. Gel filtration on Sephadex G 25 superfine was carried out on the soluble lysosomal fraction in order to investigate the stability of the 67Ga-protein complex within the lysosomes under EDTA treatment. After treatment with 1 mM/l EDTA a considerable amount of the protein bound radioactivity was found to be liberated. In further experiments the 67Ga binding lysosomal proteins were fractionated by electrophoresis on 7% polyacrylamide gels (0.5 cm x 5.5 cm). After staining with Coomassie blue 18 separated protein bands were apparent. 67Ga distribution within the gels was assessed by direct counting of radioactivity in gel slices. A considerable amount of the intralysosomal protein bound radioactivity migrated with a relative mobility of 0.36 corresponding to a protein band of molecular weight 85,000--90,000. This peak corresponded to the peak of 67Ga-labelled purified transferrin in control gels. These data were confirmed by immunoelectrophoresis combined with autoradiography: within the soluble lysosomal fraction a slight transferrin line could be identified. We conclude that 67Ga which is transported in the blood by transferrin (23) and taken up by the hepatic cell through endocytosis (32) is accumulated in the lysosomes associated with transferrin and its degraded fragments.", "PMID": 980797}
{"id": "pubmed23n0032_12523", "title": "Malabsorption of vitamin B12 in homozygous beta-thalassemia.", "content": "Schilling tests were performed in ten children and 5--12 years suffering from homozygous beta-thalassemia. 57Co labelled vitamin B12 values excreted in the urine have been found much lower than normal and remained low when the same procedure was repeated with the addition of intrinsic factor. The possible factors responsible for this malabsorption of vitamin B12 seemed to be liver damage and folic acid deficiency.", "contents": "Malabsorption of vitamin B12 in homozygous beta-thalassemia. Schilling tests were performed in ten children and 5--12 years suffering from homozygous beta-thalassemia. 57Co labelled vitamin B12 values excreted in the urine have been found much lower than normal and remained low when the same procedure was repeated with the addition of intrinsic factor. The possible factors responsible for this malabsorption of vitamin B12 seemed to be liver damage and folic acid deficiency.", "PMID": 980798}
{"id": "pubmed23n0032_12524", "title": "The influence of antidepressive drugs on the level of acetylcholine and on the acetylcholinesterase activity in the brain of rats.", "content": "Rats were treated ip with MAO inhibitos (MAO-I): nialamid (NL), pivalylbenzylhydrazine, tranylcypromine, pheniprazine (Ph) or pargyline, and the leve of total, free and bound acetylcholine (Ach) as well as the acetylcholinesterase (Ach-E) activity were estimated in four parts of rats brain 2 or 16 hr after the treatment. These parameters were estimated also after the treatment with tricyclic antidepressants: desmethylimipramine (DMI), amitriptyline, or protriptiline, and in the conditions of the reversal of reserpine-like syndrom. MAO-I, 2 hr after their application and the reversal of reserpine like-syndrom have not changed the level of measured fractions of Ach in parts of the brain. DMI increased the level of all Ach fractions in the striatum. NL caused the decrease of bound Ach level in all parts of the brain with no changes of free Ach level, 16 hr after the treatment. Ph, 16 hr after the treatment, decrease both fractions of ACh only in the cortex. All studied drugs affected evidently ACh-E activity in various parts of brain. It is concluded that:1) Cholinergic mechanisms in the rat brain are involved in the central action of DMI and of some MAO-I., 3) Cholinergic function of the brain may be modulated by the adrenergic activity, 2) Individual parts of the brain have different susceptibility to the influence of different MAO-I on the ACh-E activity.", "contents": "The influence of antidepressive drugs on the level of acetylcholine and on the acetylcholinesterase activity in the brain of rats. Rats were treated ip with MAO inhibitos (MAO-I): nialamid (NL), pivalylbenzylhydrazine, tranylcypromine, pheniprazine (Ph) or pargyline, and the leve of total, free and bound acetylcholine (Ach) as well as the acetylcholinesterase (Ach-E) activity were estimated in four parts of rats brain 2 or 16 hr after the treatment. These parameters were estimated also after the treatment with tricyclic antidepressants: desmethylimipramine (DMI), amitriptyline, or protriptiline, and in the conditions of the reversal of reserpine-like syndrom. MAO-I, 2 hr after their application and the reversal of reserpine like-syndrom have not changed the level of measured fractions of Ach in parts of the brain. DMI increased the level of all Ach fractions in the striatum. NL caused the decrease of bound Ach level in all parts of the brain with no changes of free Ach level, 16 hr after the treatment. Ph, 16 hr after the treatment, decrease both fractions of ACh only in the cortex. All studied drugs affected evidently ACh-E activity in various parts of brain. It is concluded that:1) Cholinergic mechanisms in the rat brain are involved in the central action of DMI and of some MAO-I., 3) Cholinergic function of the brain may be modulated by the adrenergic activity, 2) Individual parts of the brain have different susceptibility to the influence of different MAO-I on the ACh-E activity.", "PMID": 981020}
{"id": "pubmed23n0032_12525", "title": "Gel with nystatin for treatment of lung mycosis.", "content": "A method of preparation of the gel with nystatin for a local treatment of lung mycosis is described. Physico-chemical properties and the stability of this preparation have been investigated.", "contents": "Gel with nystatin for treatment of lung mycosis. A method of preparation of the gel with nystatin for a local treatment of lung mycosis is described. Physico-chemical properties and the stability of this preparation have been investigated.", "PMID": 981024}
{"id": "pubmed23n0032_12526", "title": "Haemodynamic changes accompanying lung oedema produced by iv injection of adrenaline or vasopressin into rats.", "content": "The blood pressure was investigated in the common left carotid artery (LC), in the left atrium (LA), in the pulmonary artery (PA) and in the right atrium (RA). After adrenaline injection the blood  pressure rised rapid and simulatneously in all four regions. After vasopressin injection the blood pressures rised sequence in LC, in LA and PA. The blood pressure in RA in this time was unchanged. These results indicate that primary causes of the lung oedema after adrenaline or vasopressin are different.", "contents": "Haemodynamic changes accompanying lung oedema produced by iv injection of adrenaline or vasopressin into rats. The blood pressure was investigated in the common left carotid artery (LC), in the left atrium (LA), in the pulmonary artery (PA) and in the right atrium (RA). After adrenaline injection the blood  pressure rised rapid and simulatneously in all four regions. After vasopressin injection the blood pressures rised sequence in LC, in LA and PA. The blood pressure in RA in this time was unchanged. These results indicate that primary causes of the lung oedema after adrenaline or vasopressin are different.", "PMID": 981023}
{"id": "pubmed23n0032_12527", "title": "Spectrophotometric and chromatographic determinations of kanamycin A in pharmaceutical preparations.", "content": "Acid hydrolysis of kanamycin A gives 5-aminomethylfurfural which can be determined quantitatively colorimetricaly by the colour complex with orcinol and FeCl3. This method gives results which correlate with the results of microbiological assay. Kanamycin B, tobramycin kasugamycin did not produce this colour reaction.", "contents": "Spectrophotometric and chromatographic determinations of kanamycin A in pharmaceutical preparations. Acid hydrolysis of kanamycin A gives 5-aminomethylfurfural which can be determined quantitatively colorimetricaly by the colour complex with orcinol and FeCl3. This method gives results which correlate with the results of microbiological assay. Kanamycin B, tobramycin kasugamycin did not produce this colour reaction.", "PMID": 981025}
{"id": "pubmed23n0032_12528", "title": "Effect of processing technique of different dosage forms on dissolution rate of caffeine and salicylamide.", "content": "The dissolution rate of caffeine (Cf) and salicylamide (SA) from the mixtures of powders, granulate and tablets, obtained by the direct compression and wet granulation methods, has been investigated. The results presented in plots show, that the dissolution process of Cf and SA follows the first-order kinetics. The kinetic equations are used for the calculation of the dissolution rate contants (K) for the compounds of different solubility. Chromatography did not give an evidence for the formation of an adduct of Cf and SA in the granulate and tablets prepared by the wet granulation method.", "contents": "Effect of processing technique of different dosage forms on dissolution rate of caffeine and salicylamide. The dissolution rate of caffeine (Cf) and salicylamide (SA) from the mixtures of powders, granulate and tablets, obtained by the direct compression and wet granulation methods, has been investigated. The results presented in plots show, that the dissolution process of Cf and SA follows the first-order kinetics. The kinetic equations are used for the calculation of the dissolution rate contants (K) for the compounds of different solubility. Chromatography did not give an evidence for the formation of an adduct of Cf and SA in the granulate and tablets prepared by the wet granulation method.", "PMID": 981026}
{"id": "pubmed23n0032_12529", "title": "Effects of manganese on substrate oxidation and oxidative phoshorylation in rat liver mitochondria.", "content": "In vitro Mn2+ decreases respiration at metabolic state III and at the uncoupled state. Pretreatment with Mn2+ decreased also ADP to oxygen ratio in rat liver and brain mitochondria. The mechanism of manganese toxicity involving suppression of substrate oxidation and decrease of oxidative phosphorylation efficiency in brain mitochondria is discussed.", "contents": "Effects of manganese on substrate oxidation and oxidative phoshorylation in rat liver mitochondria. In vitro Mn2+ decreases respiration at metabolic state III and at the uncoupled state. Pretreatment with Mn2+ decreased also ADP to oxygen ratio in rat liver and brain mitochondria. The mechanism of manganese toxicity involving suppression of substrate oxidation and decrease of oxidative phosphorylation efficiency in brain mitochondria is discussed.", "PMID": 981021}
{"id": "pubmed23n0032_12530", "title": "A search for new anticonvulsant compounds among 3-aryl-5-benzylidene derivatives of 2-thiohydantoin.", "content": "13 New 3-aryl-5-benzylidene-2-thiohydantoins were obtained by condensation of 3-o-tolyl- and 3-m-to\u0142yl-2--thiohydantoin with aromatic aldehydes. The structure of the obtained compounds was confirmed by IR spectra. Derivatives of 2-thiohydantoin 1-13 show weak anticonvulsant activity in pentetrazole test and show no protecting activity against the maximal electric shock.", "contents": "A search for new anticonvulsant compounds among 3-aryl-5-benzylidene derivatives of 2-thiohydantoin. 13 New 3-aryl-5-benzylidene-2-thiohydantoins were obtained by condensation of 3-o-tolyl- and 3-m-to\u0142yl-2--thiohydantoin with aromatic aldehydes. The structure of the obtained compounds was confirmed by IR spectra. Derivatives of 2-thiohydantoin 1-13 show weak anticonvulsant activity in pentetrazole test and show no protecting activity against the maximal electric shock.", "PMID": 981022}
{"id": "pubmed23n0032_12531", "title": "Marihuana use. Biologic and behavioral aspects.", "content": "More than 70 male chronic marihuana users were studied under research ward conditions for a 31-day period consisting of 5 days of baseline assessment, 21 days of marihuana availability, and 5 days of postsmoking assessment. Biological findings were for the most part within normal limits. Blood chemistry studies showed no abnormalities. Plasma testosterone levels for all subjects at all times during the study were well within normal limits, suggesting that previous reports of testosterone suppression by marihuana may have statistical but not biologic singificance. A significant reduction in baseline vital capacity was observed in six subjects, a compromise in pulmonary function similar to that characteristically produced by chronic inhalation of substances, such as tobacco smoke, which are irritants to the lung. Increased caloric intake and weight gain occurred in virtually all subjects and were causally related to marihuana smoking. The weight gain may be attributable to water retention as well as increased caloric intake. Behavioral findings indicated that no uniform alteration in mood is produced by marihuana smoking--all subjects reported becoming \"high\", but experienced no consistent degree of euphoria. Mood changes which occurred were relatively mild and were largely dependent on group determinants rather than individual experience. No relationship could be established between marihuana use and motivation to work or engage in socially desirable activities. Evidence that pulmonary function may be compromised as a function of marihuana smoking suggests the need for alerting individuals to this potential health hazard. As was true of cigarette smoking, the eventual public health consequences of marihuana use may become apparent only after large numbers of individuals have smoked marihuana for two or three decades.", "contents": "Marihuana use. Biologic and behavioral aspects. More than 70 male chronic marihuana users were studied under research ward conditions for a 31-day period consisting of 5 days of baseline assessment, 21 days of marihuana availability, and 5 days of postsmoking assessment. Biological findings were for the most part within normal limits. Blood chemistry studies showed no abnormalities. Plasma testosterone levels for all subjects at all times during the study were well within normal limits, suggesting that previous reports of testosterone suppression by marihuana may have statistical but not biologic singificance. A significant reduction in baseline vital capacity was observed in six subjects, a compromise in pulmonary function similar to that characteristically produced by chronic inhalation of substances, such as tobacco smoke, which are irritants to the lung. Increased caloric intake and weight gain occurred in virtually all subjects and were causally related to marihuana smoking. The weight gain may be attributable to water retention as well as increased caloric intake. Behavioral findings indicated that no uniform alteration in mood is produced by marihuana smoking--all subjects reported becoming \"high\", but experienced no consistent degree of euphoria. Mood changes which occurred were relatively mild and were largely dependent on group determinants rather than individual experience. No relationship could be established between marihuana use and motivation to work or engage in socially desirable activities. Evidence that pulmonary function may be compromised as a function of marihuana smoking suggests the need for alerting individuals to this potential health hazard. As was true of cigarette smoking, the eventual public health consequences of marihuana use may become apparent only after large numbers of individuals have smoked marihuana for two or three decades.", "PMID": 981073}
{"id": "pubmed23n0032_12532", "title": "Adaptation to chronic illness.", "content": "Adaptation to chronic illness is making the best of a bad bargain. The patient who deals successfully with chronic illness is the one who continues to live and function as fully as possible until death is appropriate and inevitable. Good medical care can help, but only if the patient meets the challenges of adaptation--acceptance, the sick role, compromise, and dying. Sometimes the patient can adapt to a new role but the family cannot; denial or nonacceptance on the part of a spouse or family can constitute a tremendous adaptational complication. Ironically, the healthy helpers around the patient, including the physician, may also fail to recognize the magnitude of the challenge of compromise and too easily criticize the patient for meeting it with difficulty or only partially.", "contents": "Adaptation to chronic illness. Adaptation to chronic illness is making the best of a bad bargain. The patient who deals successfully with chronic illness is the one who continues to live and function as fully as possible until death is appropriate and inevitable. Good medical care can help, but only if the patient meets the challenges of adaptation--acceptance, the sick role, compromise, and dying. Sometimes the patient can adapt to a new role but the family cannot; denial or nonacceptance on the part of a spouse or family can constitute a tremendous adaptational complication. Ironically, the healthy helpers around the patient, including the physician, may also fail to recognize the magnitude of the challenge of compromise and too easily criticize the patient for meeting it with difficulty or only partially.", "PMID": 981075}
{"id": "pubmed23n0032_12533", "title": "Psychologic adjustment to coronary artery disease.", "content": "As improvements in therapy have extended the survival rate of patients with ischemic heart disease, increasing attention to the quality of survival has resulted in an appreciation of the profound effect of psychologic factors on the patient's life. A multidisciplinary approach, including evaluation and management of pertient psychologic factors, should be part of the treatment of all patients with coronary heart disease. Ischemic heart disease often requires both patient and family to make difficult changes in personality and life-style. As soon as the physiologic stress begins to subside, the physician should begin to provide sympathetic, specific guidelines toward acceptance of the disease and accommodation to its limits. Potential problems and their management are discussed within the framework of the three stages of chronic illness.", "contents": "Psychologic adjustment to coronary artery disease. As improvements in therapy have extended the survival rate of patients with ischemic heart disease, increasing attention to the quality of survival has resulted in an appreciation of the profound effect of psychologic factors on the patient's life. A multidisciplinary approach, including evaluation and management of pertient psychologic factors, should be part of the treatment of all patients with coronary heart disease. Ischemic heart disease often requires both patient and family to make difficult changes in personality and life-style. As soon as the physiologic stress begins to subside, the physician should begin to provide sympathetic, specific guidelines toward acceptance of the disease and accommodation to its limits. Potential problems and their management are discussed within the framework of the three stages of chronic illness.", "PMID": 981077}
{"id": "pubmed23n0032_12534", "title": "Psychiatric aspects of chronic organic brain syndrome.", "content": "In chronic organic brain syndrome, or dementia, the patient generally retreats to simple, familiar situations and resists involvement in others. The symptoms represent both organic deficits due to brain damage and psychologic reactions to the deficits. Some causes are treatable. Because of progressive change in the total behavior of the patient, major rearrangement of life-style is often necessary. The physician should guide both patient and family in this process. Medication may also be helpful.", "contents": "Psychiatric aspects of chronic organic brain syndrome. In chronic organic brain syndrome, or dementia, the patient generally retreats to simple, familiar situations and resists involvement in others. The symptoms represent both organic deficits due to brain damage and psychologic reactions to the deficits. Some causes are treatable. Because of progressive change in the total behavior of the patient, major rearrangement of life-style is often necessary. The physician should guide both patient and family in this process. Medication may also be helpful.", "PMID": 981078}
{"id": "pubmed23n0032_12535", "title": "Cardiology. acute severe mitral regurgitation.", "content": "Acute mitral regurgitation occurs as a complication of myocardial infarction in perhaps 1% of cases. The characteristic clinical findings include a new systolic murmur occurring in the setting of sudden development of acute congestive heart failure, hypotension, and shock. S3 and S4 gallops and sinus rhythm usually are present, and gross left ventricular or left atrial enlargement usually is not evident radiologically. The immediate and long-term prognoses for patients with acute severe mitral regurgitation are poor without treatment. Although medical therapy can reverse temporarily some of the hemodynamic aberrations, it does not influence survival or eliminate the need for surgical treatment. Surgery consisting of mitral valve replacement and possibly simultaneous coronary revascularization appears to offer some hope in increasing the survival rate among these patients. When acute severe mitral regurgitation is secondary to rupture of chordae tendineae in circumstances other than coronary artery disease, the outlook is not as grim. These patients may show signs and symptoms of congestive heart failure for a few weeks or, at times, many months. Again, sinus rhythm usually is present, with slight left ventircular enlargement, a relatively normal left atrial size, and markedly elevated left atrial and pulmonary arterial pressures. This constellation of findings is indicative of a large mitral regurgitant flow with preservation of left ventricular function and suggests that the patient is likely to benefit greatly from surgical treatment. The prognosis for such patients is much better than it is for the patients with acute mitral regurgitation secondary to coronary artery disease.", "contents": "Cardiology. acute severe mitral regurgitation. Acute mitral regurgitation occurs as a complication of myocardial infarction in perhaps 1% of cases. The characteristic clinical findings include a new systolic murmur occurring in the setting of sudden development of acute congestive heart failure, hypotension, and shock. S3 and S4 gallops and sinus rhythm usually are present, and gross left ventricular or left atrial enlargement usually is not evident radiologically. The immediate and long-term prognoses for patients with acute severe mitral regurgitation are poor without treatment. Although medical therapy can reverse temporarily some of the hemodynamic aberrations, it does not influence survival or eliminate the need for surgical treatment. Surgery consisting of mitral valve replacement and possibly simultaneous coronary revascularization appears to offer some hope in increasing the survival rate among these patients. When acute severe mitral regurgitation is secondary to rupture of chordae tendineae in circumstances other than coronary artery disease, the outlook is not as grim. These patients may show signs and symptoms of congestive heart failure for a few weeks or, at times, many months. Again, sinus rhythm usually is present, with slight left ventircular enlargement, a relatively normal left atrial size, and markedly elevated left atrial and pulmonary arterial pressures. This constellation of findings is indicative of a large mitral regurgitant flow with preservation of left ventricular function and suggests that the patient is likely to benefit greatly from surgical treatment. The prognosis for such patients is much better than it is for the patients with acute mitral regurgitation secondary to coronary artery disease.", "PMID": 981083}
{"id": "pubmed23n0032_12536", "title": "Office treatment of eye injury. 2. Injury from sharp instruments or blunt trauma.", "content": "Eye injuries caused by sharp instruments range from superficial scratches of the corneal epithelium to serious lacerations of the globe of the eye. Scratches, conjunctival lacerations, and some eyelid lacerations can be dealth with by the primary physician in the office, but damage to the globe requires immediate referral to an ophthalmologist. Among possible results of blunt trauma to the area of the eye are ecchymosis, hyphema, blow-out fracture, subluxation or dislocation of the lens, or retinal detachment. Most of thes require thoroguh ophthalmologic examination and specialized treatment. The primary physician may be the first to examine a patient with serious head injury. For future reference the status of each eye should be carefully documented as soon as possible after injury.", "contents": "Office treatment of eye injury. 2. Injury from sharp instruments or blunt trauma. Eye injuries caused by sharp instruments range from superficial scratches of the corneal epithelium to serious lacerations of the globe of the eye. Scratches, conjunctival lacerations, and some eyelid lacerations can be dealth with by the primary physician in the office, but damage to the globe requires immediate referral to an ophthalmologist. Among possible results of blunt trauma to the area of the eye are ecchymosis, hyphema, blow-out fracture, subluxation or dislocation of the lens, or retinal detachment. Most of thes require thoroguh ophthalmologic examination and specialized treatment. The primary physician may be the first to examine a patient with serious head injury. For future reference the status of each eye should be carefully documented as soon as possible after injury.", "PMID": 981084}
{"id": "pubmed23n0032_12537", "title": "Acute acid-base disorders. 2. Specific disturbances.", "content": "Evaluation of the acid-base status of the body requires measurement of bicarbonate (total carbon dioxide) concentration, pH, and partial pressure of CO2 in arterial blood. Calculation of standard bicarbonate and base excess or deficit is not necessary. The normal concentration of free hydrogen ions (H+) is approximately 40 millimoles/liter, which is equivalent to a pH of 7.4. The normal load of fixed acids is 50 to 80 millimoles in 24 hours. A steady state is maintained by excretion of an equal amount of H+ by the kidneys, which at the same time regenerate bicarbonate to replenish buffer stores. Renal excretion of H+ is in the form of titratable acid and ammonium. Synthesis of ammonia can increase severalfold under the stimulus of acidosis. This is the chief mechanism of long-term compensation. Metabolic acidosis can be due to an excessive acid load (endogenous or exogenous), impaired renal excretion of H+, or bicarbonate loss. Determination of the \"anion gap\" (unmeasured anions) helps to establish the mechanism of acidosis. Acidosis with a normal anion gap is due to either bicarbonate loss or ingestion of certain chloride salts. A gap larger than normal indicates the presence in the body of acids other than acidfying chloride salts. Management of metabolic acidosis requires accurate diagnosis, clear understanding of the mechansim, and individualized treatment. Metabloic alkalosis is due to loss of H+ (usually from stomach or kidneys) or ingestion of alkali. Measurement of urinary chloride helps establish the mechanism of alkalosis. In saline-responsive alkalosis, the urinary chloride level is very low. This is usually due to gastric loss of H+, and the condition responds to administration of saline solution. When the urinary chloride level is only moderately low, the alkalosis is probably not due to gastric loss of H+. This form of alkalosis (saline-resistant) does not respond well to administration of saline solution and requires use of potassium in treatment. Apprpriate compensatory responses to acidosis or alkalosis are critical to survival. Compensation for metabloic acidosis consists of hyperventilation and enhanced renal excretion of H+, chiefly as ammonium. In metabolic alkalosis, compensation is mainly renal excretion of bicarbonate. Respiratory acidosis is due to alveolar hypoventilation. In chronic situations, a compensatory rise in serum bicarbonate concentration is expected. Management consists of treatment of the cause of hypoventilation. Respiratory alkalosis is due to hyperventilation. Treatment requires identification and correction of the cause of hyperventilation.", "contents": "Acute acid-base disorders. 2. Specific disturbances. Evaluation of the acid-base status of the body requires measurement of bicarbonate (total carbon dioxide) concentration, pH, and partial pressure of CO2 in arterial blood. Calculation of standard bicarbonate and base excess or deficit is not necessary. The normal concentration of free hydrogen ions (H+) is approximately 40 millimoles/liter, which is equivalent to a pH of 7.4. The normal load of fixed acids is 50 to 80 millimoles in 24 hours. A steady state is maintained by excretion of an equal amount of H+ by the kidneys, which at the same time regenerate bicarbonate to replenish buffer stores. Renal excretion of H+ is in the form of titratable acid and ammonium. Synthesis of ammonia can increase severalfold under the stimulus of acidosis. This is the chief mechanism of long-term compensation. Metabolic acidosis can be due to an excessive acid load (endogenous or exogenous), impaired renal excretion of H+, or bicarbonate loss. Determination of the \"anion gap\" (unmeasured anions) helps to establish the mechanism of acidosis. Acidosis with a normal anion gap is due to either bicarbonate loss or ingestion of certain chloride salts. A gap larger than normal indicates the presence in the body of acids other than acidfying chloride salts. Management of metabolic acidosis requires accurate diagnosis, clear understanding of the mechansim, and individualized treatment. Metabloic alkalosis is due to loss of H+ (usually from stomach or kidneys) or ingestion of alkali. Measurement of urinary chloride helps establish the mechanism of alkalosis. In saline-responsive alkalosis, the urinary chloride level is very low. This is usually due to gastric loss of H+, and the condition responds to administration of saline solution. When the urinary chloride level is only moderately low, the alkalosis is probably not due to gastric loss of H+. This form of alkalosis (saline-resistant) does not respond well to administration of saline solution and requires use of potassium in treatment. Apprpriate compensatory responses to acidosis or alkalosis are critical to survival. Compensation for metabloic acidosis consists of hyperventilation and enhanced renal excretion of H+, chiefly as ammonium. In metabolic alkalosis, compensation is mainly renal excretion of bicarbonate. Respiratory acidosis is due to alveolar hypoventilation. In chronic situations, a compensatory rise in serum bicarbonate concentration is expected. Management consists of treatment of the cause of hypoventilation. Respiratory alkalosis is due to hyperventilation. Treatment requires identification and correction of the cause of hyperventilation.", "PMID": 981088}
{"id": "pubmed23n0032_12538", "title": "Cold agglutinin disease.", "content": "Development of the Coombs test has led to rapid progress in the recognition of various autoimmune hemolytic anemias. Cold agglutinin disease, a relatively rare disorder, occurs in idiopathic and secondary forms, the latter often in association with diseases that alter the immune status. The higher the titer of cold agglutinin, the greater is the thermal range of its activity. One of the characteristic clinical manifestations of the disease is striking cyanosis in acral areas on exposure to cold, which abates quickly on warming. This parallels the accelerated hemolysis at cold temperatures, which is reversible as heat is applied. In some patients, however, the disease is covert and is discovered only incidentally. The report of such a case is included.", "contents": "Cold agglutinin disease. Development of the Coombs test has led to rapid progress in the recognition of various autoimmune hemolytic anemias. Cold agglutinin disease, a relatively rare disorder, occurs in idiopathic and secondary forms, the latter often in association with diseases that alter the immune status. The higher the titer of cold agglutinin, the greater is the thermal range of its activity. One of the characteristic clinical manifestations of the disease is striking cyanosis in acral areas on exposure to cold, which abates quickly on warming. This parallels the accelerated hemolysis at cold temperatures, which is reversible as heat is applied. In some patients, however, the disease is covert and is discovered only incidentally. The report of such a case is included.", "PMID": 981089}
{"id": "pubmed23n0032_12539", "title": "Genetics of ischaemic heart disease.", "content": "Coronary heart disease, especially when it affects younger individuals, tends to cluster in families. Known risk factors occur in 50-75% of patients with myocardial infarction. Yet commonly occurring risk factors are not strongly inherited, and the familial aggregation of coronary heart diseas may not be attributable to familial resemblance in serum cholesterol and blood pressure levels. Alternatively, such aggregation may be due to unknown familial risk factors. Nevertheless, screening of relatives of individuals with evident risk factors is of importance, and environmental manipulation is likely to be of value in prevention of coronary heart disease.", "contents": "Genetics of ischaemic heart disease. Coronary heart disease, especially when it affects younger individuals, tends to cluster in families. Known risk factors occur in 50-75% of patients with myocardial infarction. Yet commonly occurring risk factors are not strongly inherited, and the familial aggregation of coronary heart diseas may not be attributable to familial resemblance in serum cholesterol and blood pressure levels. Alternatively, such aggregation may be due to unknown familial risk factors. Nevertheless, screening of relatives of individuals with evident risk factors is of importance, and environmental manipulation is likely to be of value in prevention of coronary heart disease.", "PMID": 981090}
{"id": "pubmed23n0032_12540", "title": "Exertional hypotension due to postganglionic sympathetic blocking drugs.", "content": "Debrisoquine, guanethidine and bethanidine may produce troublesome hypotensive symtoms related to exertion. Thirteen patients with such symptoms were exercised on a treadmill and the response of blood pressure and heart rate was compared to that of thirty patients without these symptoms, who were exercised to the same extent. There was a slight drop of systolic and diastolic pressures on standing in both groups, but after exertion there was a significantly greater drop of systolic pressure in the group with symptoms than in the asymptomatic group. The diastolic pressure after exertion was significantly lower in the group with symptoms. It was impossible to predict from the standing blood pressure levels at rest which patients would develop hypotensive symptoms after exertion. All three drugs had a similar negative chronotropic effect at rest and on exercise. It is suggested that patients are exercised during control of hypertension in order to identify those prone to exertional hypotension. Patients with such hypotension should be exercised on each attendance before the blood pressure is measured. Treatment other than postganglionic sympathetic blocking drugs should be employed whenever possible in patients with milder hypertension.", "contents": "Exertional hypotension due to postganglionic sympathetic blocking drugs. Debrisoquine, guanethidine and bethanidine may produce troublesome hypotensive symtoms related to exertion. Thirteen patients with such symptoms were exercised on a treadmill and the response of blood pressure and heart rate was compared to that of thirty patients without these symptoms, who were exercised to the same extent. There was a slight drop of systolic and diastolic pressures on standing in both groups, but after exertion there was a significantly greater drop of systolic pressure in the group with symptoms than in the asymptomatic group. The diastolic pressure after exertion was significantly lower in the group with symptoms. It was impossible to predict from the standing blood pressure levels at rest which patients would develop hypotensive symptoms after exertion. All three drugs had a similar negative chronotropic effect at rest and on exercise. It is suggested that patients are exercised during control of hypertension in order to identify those prone to exertional hypotension. Patients with such hypotension should be exercised on each attendance before the blood pressure is measured. Treatment other than postganglionic sympathetic blocking drugs should be employed whenever possible in patients with milder hypertension.", "PMID": 981091}
{"id": "pubmed23n0032_12541", "title": "Pregnancy following poliomyelitis.", "content": "A review of forty-nine pregnancies in thirty-seven patients who had previously suffered from poliomyelitis found that obstetric complications were proportional to the clinical disabilities of the patient. A simple test of unilateral weight-bearing gives a good clue as to whether pelvic asymmetry is present.", "contents": "Pregnancy following poliomyelitis. A review of forty-nine pregnancies in thirty-seven patients who had previously suffered from poliomyelitis found that obstetric complications were proportional to the clinical disabilities of the patient. A simple test of unilateral weight-bearing gives a good clue as to whether pelvic asymmetry is present.", "PMID": 981092}
{"id": "pubmed23n0032_12542", "title": "Levamisole treatment of a child with severe aphthous stomatitis and neutropenia.", "content": "The treatment of a 12-year-old girl with a lifelone history of recurrent infections and aphthous stomatitis is reported. A profound neutropenis, first noted at the age of 2 years, occurring at least every month was observed together with multiple mouth ulcers a sore throat and swelling of the jugular glands. Levamisole, originally described as an anthelmintic, has a beneficial effect on the symptoms of recurrent aphthous stomatitis. After levamisole treatment aphthous stomatitis was milder and in the 1-year follow-up period the patient was asymptomatic several times during a phase of obvious neutrophil depression. The child no longer complained of a sore throat with swelling of the jugular glands and the recurrent staphylococcal infections of the skin disappeared. After therapy a marked increase in monocytes at the moment of neutropenia was observed.", "contents": "Levamisole treatment of a child with severe aphthous stomatitis and neutropenia. The treatment of a 12-year-old girl with a lifelone history of recurrent infections and aphthous stomatitis is reported. A profound neutropenis, first noted at the age of 2 years, occurring at least every month was observed together with multiple mouth ulcers a sore throat and swelling of the jugular glands. Levamisole, originally described as an anthelmintic, has a beneficial effect on the symptoms of recurrent aphthous stomatitis. After levamisole treatment aphthous stomatitis was milder and in the 1-year follow-up period the patient was asymptomatic several times during a phase of obvious neutrophil depression. The child no longer complained of a sore throat with swelling of the jugular glands and the recurrent staphylococcal infections of the skin disappeared. After therapy a marked increase in monocytes at the moment of neutropenia was observed.", "PMID": 981093}
{"id": "pubmed23n0032_12543", "title": "Acute coronary insufficiency with normal coronary arteriograms.", "content": "Two patients presenting with apparent symptomatic and electrocardiographic evidence of acute coronary insufficiency and found to have normal coronary arteriograms are documented. The patients remain symptom free at follow-up 9 and 14 months later although one requires 160 mg propranolol daily. The electrocardiographic, myocardial metabolic and haemodynamic data in 413 patients with angina and normal coronary arteriograms are reviewed and the suggested aetiologies documented. Follow-up of patients in these series indicates a good prognosis for symptom-free survival.", "contents": "Acute coronary insufficiency with normal coronary arteriograms. Two patients presenting with apparent symptomatic and electrocardiographic evidence of acute coronary insufficiency and found to have normal coronary arteriograms are documented. The patients remain symptom free at follow-up 9 and 14 months later although one requires 160 mg propranolol daily. The electrocardiographic, myocardial metabolic and haemodynamic data in 413 patients with angina and normal coronary arteriograms are reviewed and the suggested aetiologies documented. Follow-up of patients in these series indicates a good prognosis for symptom-free survival.", "PMID": 981094}
{"id": "pubmed23n0032_12544", "title": "Giant cell arteritis presenting as subclavian artery occlusion. A report of two cases.", "content": "Two patients with the aortic arch syndrome and biopsy-proved but asymptomatic temporal giant cell arteritis are described. Corticosteroid treatment was followed by the return of peripheral pulsation in these two patients after an interval of a few months. A review of the literature suggests that of patients where giant cell arteritis causes subclavin obstruction there is an undue predominance of women, compared to the sex ratio found in giant cell arteritis overall.", "contents": "Giant cell arteritis presenting as subclavian artery occlusion. A report of two cases. Two patients with the aortic arch syndrome and biopsy-proved but asymptomatic temporal giant cell arteritis are described. Corticosteroid treatment was followed by the return of peripheral pulsation in these two patients after an interval of a few months. A review of the literature suggests that of patients where giant cell arteritis causes subclavin obstruction there is an undue predominance of women, compared to the sex ratio found in giant cell arteritis overall.", "PMID": 981095}
{"id": "pubmed23n0032_12545", "title": "Pulmonary oedema in meningococcal meningitis.", "content": "Two cases of meningococcal meningitis complicated by pulmonary oedema are described. The pulmonary arterial wedge pressure was raised in the one case studied. Profound sympathetic over-activity may be the cause of the pulmonary oedema occurring in this situation. If this is so, adrenergic blockade would appear to be a rational approach to therapy.", "contents": "Pulmonary oedema in meningococcal meningitis. Two cases of meningococcal meningitis complicated by pulmonary oedema are described. The pulmonary arterial wedge pressure was raised in the one case studied. Profound sympathetic over-activity may be the cause of the pulmonary oedema occurring in this situation. If this is so, adrenergic blockade would appear to be a rational approach to therapy.", "PMID": 981096}
{"id": "pubmed23n0032_12546", "title": "Pulmonary oedema during treatment of acute water intoxication.", "content": "Acute water intoxication with deepening coma and uncontrolled epileptiform seizures in a 25-year-old previously fit male schizophrenic was treated with hypertonic (2 N) saline and a 20% mannitol solution. This improved his neurological state but precipitated severe pulmonary oedema. Intravenous frusemide increased his urinary output sufficiently to clear the pulmonary oedema. In acute water intoxication the use of hypertonic solutions may thus precipitate left heart failure by expanding the intra-pulmonary blood volume beyond the capacity of even a healthy left ventricle to compensate. Simple water restriction will produce a slower but perhaps safer improvement.", "contents": "Pulmonary oedema during treatment of acute water intoxication. Acute water intoxication with deepening coma and uncontrolled epileptiform seizures in a 25-year-old previously fit male schizophrenic was treated with hypertonic (2 N) saline and a 20% mannitol solution. This improved his neurological state but precipitated severe pulmonary oedema. Intravenous frusemide increased his urinary output sufficiently to clear the pulmonary oedema. In acute water intoxication the use of hypertonic solutions may thus precipitate left heart failure by expanding the intra-pulmonary blood volume beyond the capacity of even a healthy left ventricle to compensate. Simple water restriction will produce a slower but perhaps safer improvement.", "PMID": 981097}
{"id": "pubmed23n0032_12547", "title": "Contraceptive pill-induced porphyria cutanea tarda presenting with onycholysis of the finger nails.", "content": "A case of porphyria cutanea tarda induced by oral contraceptive pill therapy is reported, and its unusual presenting sign of onycholysis of the finger nails is noted. Reference is made to the rarity of nail changes in porphyria and the regression of the onycholysis in the patient following therapy is outlined.", "contents": "Contraceptive pill-induced porphyria cutanea tarda presenting with onycholysis of the finger nails. A case of porphyria cutanea tarda induced by oral contraceptive pill therapy is reported, and its unusual presenting sign of onycholysis of the finger nails is noted. Reference is made to the rarity of nail changes in porphyria and the regression of the onycholysis in the patient following therapy is outlined.", "PMID": 981098}
{"id": "pubmed23n0032_12548", "title": "[Pulmonary complications of azotemia. A case of uremic lung].", "content": "The authors studied the problem of pulmonary complications in acute and chronic uremia. They recalled the main characteristics of haemodynamic oedemas which can accompany the periods of left ventricular insufficiency during the evolution of uremia. They analized the characteristicsof the classic uremic lung representing in fact only a variety of lesional oedema in which the toxic effect of urea is controversial. Ventilatory consequences of chronic uremia were briefly exposed.", "contents": "[Pulmonary complications of azotemia. A case of uremic lung]. The authors studied the problem of pulmonary complications in acute and chronic uremia. They recalled the main characteristics of haemodynamic oedemas which can accompany the periods of left ventricular insufficiency during the evolution of uremia. They analized the characteristicsof the classic uremic lung representing in fact only a variety of lesional oedema in which the toxic effect of urea is controversial. Ventilatory consequences of chronic uremia were briefly exposed.", "PMID": 981124}
{"id": "pubmed23n0032_12549", "title": "[Congenital isolated unilateral absence of the left pulmonary artery with dextrorotation of the aorta (2 cases)].", "content": "In connection with two cases of congenital absence of the left pulmonary artery with dextro-rotation of the aorta, the authors recall the clinical and radiological signs of this rare abnormality, explaining it by the theory of failure in partitioning of the troncus arteriosus. They particularly insist on the normal pulmonary venous return.", "contents": "[Congenital isolated unilateral absence of the left pulmonary artery with dextrorotation of the aorta (2 cases)]. In connection with two cases of congenital absence of the left pulmonary artery with dextro-rotation of the aorta, the authors recall the clinical and radiological signs of this rare abnormality, explaining it by the theory of failure in partitioning of the troncus arteriosus. They particularly insist on the normal pulmonary venous return.", "PMID": 981125}
{"id": "pubmed23n0032_12550", "title": "[Treatment of bronchial hypersecretion. Demonstration of polyanionic mucins-eprazinone interactions].", "content": "The study of therapeutical agents for \"mucolysis\" enables a classification of these drugs from their mode of action at the trachea-bronchial level (pharmacodynamic agents, enzymes, reducers, mucoregulation). The affinity of a piperazine derivative, eprazinone, for polyanionic bronchial mucins, was shown in vitro by spectrophotometry. Because of this property, eprazinone (Mucitux) might modify the fibrillar structure of bronchial mucus. This mode of action would differentiate this treatment from the mucolytic agents used today clinically.", "contents": "[Treatment of bronchial hypersecretion. Demonstration of polyanionic mucins-eprazinone interactions]. The study of therapeutical agents for \"mucolysis\" enables a classification of these drugs from their mode of action at the trachea-bronchial level (pharmacodynamic agents, enzymes, reducers, mucoregulation). The affinity of a piperazine derivative, eprazinone, for polyanionic bronchial mucins, was shown in vitro by spectrophotometry. Because of this property, eprazinone (Mucitux) might modify the fibrillar structure of bronchial mucus. This mode of action would differentiate this treatment from the mucolytic agents used today clinically.", "PMID": 981126}
{"id": "pubmed23n0032_12551", "title": "[Aspiration-drainage of an emphysematous bulla in a patient with severe respiratory insufficiency].", "content": "The authors report the case of a patient with severe alveolar hypoventilation, with a large emphysematous bulla in the upper part of the right pulmonary field. The drainage aspiration of the bulla as a life saving technique greatly improved the respiratory function. Blood gases reverted to normal and the bullae disappeared. Literature reviewing. Discussion of radiological and functional criteria of compressing the pulmonary parenchyma around the bulla.", "contents": "[Aspiration-drainage of an emphysematous bulla in a patient with severe respiratory insufficiency]. The authors report the case of a patient with severe alveolar hypoventilation, with a large emphysematous bulla in the upper part of the right pulmonary field. The drainage aspiration of the bulla as a life saving technique greatly improved the respiratory function. Blood gases reverted to normal and the bullae disappeared. Literature reviewing. Discussion of radiological and functional criteria of compressing the pulmonary parenchyma around the bulla.", "PMID": 981127}
{"id": "pubmed23n0032_12552", "title": "[Respiratory distress in evolutive tuberculosis (6 cases)].", "content": "The authors reported six cases of respiratory distress of tuberculous origin over a 3 year period. They underlined the failure to recognize the etiology despite a typical symptomatology and, on the opposite, the diagnostic difficulties met in a former tuberculous patient with chronic bronchitis. They insisted on the persistance of evolutive tuberculosis as a cause of respiratory distress, on the importance of any associated infection and on the little use of assisted ventilation.", "contents": "[Respiratory distress in evolutive tuberculosis (6 cases)]. The authors reported six cases of respiratory distress of tuberculous origin over a 3 year period. They underlined the failure to recognize the etiology despite a typical symptomatology and, on the opposite, the diagnostic difficulties met in a former tuberculous patient with chronic bronchitis. They insisted on the persistance of evolutive tuberculosis as a cause of respiratory distress, on the importance of any associated infection and on the little use of assisted ventilation.", "PMID": 981128}
{"id": "pubmed23n0032_12553", "title": "The surgical treatment of hyperhidrosis.", "content": "The diagnosis of primary hyperhidrosis is one of exclusion, but it is not a difficult one to make. It is a common, embarrassing and even disabling condition. If it does not respond to simple conservative therapy, the practitioner should not hesitate to advise sympathectomy for hyperhidrosis of the upper or lower limbs, or some simple local operation on the affected skin area for localized excessive axillary sweating.", "contents": "The surgical treatment of hyperhidrosis. The diagnosis of primary hyperhidrosis is one of exclusion, but it is not a difficult one to make. It is a common, embarrassing and even disabling condition. If it does not respond to simple conservative therapy, the practitioner should not hesitate to advise sympathectomy for hyperhidrosis of the upper or lower limbs, or some simple local operation on the affected skin area for localized excessive axillary sweating.", "PMID": 981132}
{"id": "pubmed23n0032_12554", "title": "A new waist-length lightweight elastic stocking.", "content": "This trial has clearly demonstrated that the new waist-length lightweight elastic stocking introduces advantages over the normal thigh-length lightweight elastic stocking, with regard to comfort and appearance, and over support tights by applying greater pressure. Since they conform to current fashion they are more easily accepted by women patients, who preferred them to the present thigh-length elastic hosiery.", "contents": "A new waist-length lightweight elastic stocking. This trial has clearly demonstrated that the new waist-length lightweight elastic stocking introduces advantages over the normal thigh-length lightweight elastic stocking, with regard to comfort and appearance, and over support tights by applying greater pressure. Since they conform to current fashion they are more easily accepted by women patients, who preferred them to the present thigh-length elastic hosiery.", "PMID": 981133}
{"id": "pubmed23n0032_12555", "title": "[Changes in 2,3-diphosphoglycerate Levels in Blood and Brain Tissue after Craniocerebral Trauma and Cardiac Surgery].", "content": "The response of the 2,3-diphosphoglycerate (DPG) levels in the blood and brain tissue to a craniocerebral trauma of varying severity was studied in anaesthetized rats. A trauma producing cerebral contusion was followed within two hours by a highly significant rise in DPG concentration in the blood as compared with the control animals or only mildly traumatized rats. The DPG levels in the brain tissue showed no significant differences. Similar changes in DPG concentration were observed in the blood of patients with craniocerebral injuries. The DPG-mediated increased release of oxygen to the tissues represents a compensatory mechanism and is pathognomic for craniocerebral trauma. Patients undergoing surgery with extracorporeal circulation lack this mechanism for counteracting hypoxaemia; already during thoracotomy the DPG concentration in the blood fell significantly and did not reach its original level until 72 hours after the operation. In stored, ACD stabilized, blood the DPG concentration gradually decreases. Estimations carried out over 28 days showed a continuous statistically significant loss of DPG. After 24 hours the DPG levels in stored blood had already dropped to the lower limits of normal - a fact that has to be taken into account in massive blood transfusions.", "contents": "[Changes in 2,3-diphosphoglycerate Levels in Blood and Brain Tissue after Craniocerebral Trauma and Cardiac Surgery]. The response of the 2,3-diphosphoglycerate (DPG) levels in the blood and brain tissue to a craniocerebral trauma of varying severity was studied in anaesthetized rats. A trauma producing cerebral contusion was followed within two hours by a highly significant rise in DPG concentration in the blood as compared with the control animals or only mildly traumatized rats. The DPG levels in the brain tissue showed no significant differences. Similar changes in DPG concentration were observed in the blood of patients with craniocerebral injuries. The DPG-mediated increased release of oxygen to the tissues represents a compensatory mechanism and is pathognomic for craniocerebral trauma. Patients undergoing surgery with extracorporeal circulation lack this mechanism for counteracting hypoxaemia; already during thoracotomy the DPG concentration in the blood fell significantly and did not reach its original level until 72 hours after the operation. In stored, ACD stabilized, blood the DPG concentration gradually decreases. Estimations carried out over 28 days showed a continuous statistically significant loss of DPG. After 24 hours the DPG levels in stored blood had already dropped to the lower limits of normal - a fact that has to be taken into account in massive blood transfusions.", "PMID": 981139}
{"id": "pubmed23n0032_12556", "title": "[Isoimmune antibody formation after blood transfusions].", "content": "During February 1973 to October 1974 950 inpatients of the Department of Surgery, Erlangen, were tested for the presence of iso-antibodies before and after blood transfusions. Irregular antibodies were demonstrable in 0.95% of the patients before they received a blood transfusion. In 2.73% of the cases blood transfusion resulted in sensitization with the formation of specific blood group antibodies. Identification of the 26 iso-antibodies showed a prevalence of anti-Kell and anti-E (7 cases each). In view of the frequent occurrence of these two antigens tests for their presence are advisable before a blood transfusion is given. Persons who are re-admitted to hospital should also be tested to the presence of irregular antibodies to ensure that sensitization has not occurred during the intervening period.", "contents": "[Isoimmune antibody formation after blood transfusions]. During February 1973 to October 1974 950 inpatients of the Department of Surgery, Erlangen, were tested for the presence of iso-antibodies before and after blood transfusions. Irregular antibodies were demonstrable in 0.95% of the patients before they received a blood transfusion. In 2.73% of the cases blood transfusion resulted in sensitization with the formation of specific blood group antibodies. Identification of the 26 iso-antibodies showed a prevalence of anti-Kell and anti-E (7 cases each). In view of the frequent occurrence of these two antigens tests for their presence are advisable before a blood transfusion is given. Persons who are re-admitted to hospital should also be tested to the presence of irregular antibodies to ensure that sensitization has not occurred during the intervening period.", "PMID": 981140}
{"id": "pubmed23n0032_12557", "title": "[Clinical hemodilution].", "content": "Acute hemodilution has been performed in 46 patients immediately before major gastrointestinal surgery. Clinical, hemodynamic, respiratory and metabolic parameters have been studied and compared with results in 66 patients, submitted to similar operations under equal conditions without hemodilution. Two kinds of hemodilution have been used: normovolamic hemodilution by bloodexchange with 5% human albumin and moderate hypervolamic hemodilution by bloodexchange with equal parts of Dextran 60 and PPL. 1. The first mentioned kind of hemodilution and method of blood-exchange was superior in regard to stability of cardiovascular behavior. 2. The general tolerance of anesthesia and surgical trauma was superior in the nonhemodiluted group, but was good or tolerable also in the hemodiluted group in 82,6%. Side effects, more frequently and more pronounced in the hemodiluted group concerned to hypo- and hypertensive reactions, vasoconstriction, hypokaliemie and cardial arrhythmics. Exclusively in the hemodiluted group ST-lowering in the EKG has been stated in some patients. These reactions have been estimated as a sign of marginal compensatory capacity in the individual cardial situation and were reversibel by blood transfusion. These observations strengthen the necessity for accurate monitoring in hemodiluted patients. Moreover it is recommended to restrict hemodilution in the level of Hk 27-28%. Under these conditions acute normovolamic hemodilution is recommended further for a wider clinical use.", "contents": "[Clinical hemodilution]. Acute hemodilution has been performed in 46 patients immediately before major gastrointestinal surgery. Clinical, hemodynamic, respiratory and metabolic parameters have been studied and compared with results in 66 patients, submitted to similar operations under equal conditions without hemodilution. Two kinds of hemodilution have been used: normovolamic hemodilution by bloodexchange with 5% human albumin and moderate hypervolamic hemodilution by bloodexchange with equal parts of Dextran 60 and PPL. 1. The first mentioned kind of hemodilution and method of blood-exchange was superior in regard to stability of cardiovascular behavior. 2. The general tolerance of anesthesia and surgical trauma was superior in the nonhemodiluted group, but was good or tolerable also in the hemodiluted group in 82,6%. Side effects, more frequently and more pronounced in the hemodiluted group concerned to hypo- and hypertensive reactions, vasoconstriction, hypokaliemie and cardial arrhythmics. Exclusively in the hemodiluted group ST-lowering in the EKG has been stated in some patients. These reactions have been estimated as a sign of marginal compensatory capacity in the individual cardial situation and were reversibel by blood transfusion. These observations strengthen the necessity for accurate monitoring in hemodiluted patients. Moreover it is recommended to restrict hemodilution in the level of Hk 27-28%. Under these conditions acute normovolamic hemodilution is recommended further for a wider clinical use.", "PMID": 981141}
{"id": "pubmed23n0032_12558", "title": "[Clinical uses of ultraviolet light radiation].", "content": "The main use of ultraviolet light radiation in hospitals is for the disinfection of the air. A study was made of the disinfecting efficiency of indirect ultraviolet radiation (lifts, cleaning of bedding), direct irradiation of rooms overnight or after use, ultraviolet curtains (irradiation of the whole width of passages, doorways and entrances) and ultraviolet irradiation of the filter of air-conditioners. The results are analysed. With the exception of the barrier technique ultraviolet light irradiation did not substantially reduce the number of micro-organisms in the air. Knowledge of the limitations and side-effects of this technique, control of the intensity of ultraviolet irradiation and bacterial counts are regular intervals are some of the factors that determine the success of this mode of disinfection. Detailed studies of the acceptable upper limits of the number of micro-organisms in the air of hospital wards and operating theatres is of prime importance for evaluating the effectiveness of air-disinfection by ultraviolet light irradiation.", "contents": "[Clinical uses of ultraviolet light radiation]. The main use of ultraviolet light radiation in hospitals is for the disinfection of the air. A study was made of the disinfecting efficiency of indirect ultraviolet radiation (lifts, cleaning of bedding), direct irradiation of rooms overnight or after use, ultraviolet curtains (irradiation of the whole width of passages, doorways and entrances) and ultraviolet irradiation of the filter of air-conditioners. The results are analysed. With the exception of the barrier technique ultraviolet light irradiation did not substantially reduce the number of micro-organisms in the air. Knowledge of the limitations and side-effects of this technique, control of the intensity of ultraviolet irradiation and bacterial counts are regular intervals are some of the factors that determine the success of this mode of disinfection. Detailed studies of the acceptable upper limits of the number of micro-organisms in the air of hospital wards and operating theatres is of prime importance for evaluating the effectiveness of air-disinfection by ultraviolet light irradiation.", "PMID": 981142}
{"id": "pubmed23n0032_12559", "title": "[Succinyldicholin sensitivity resulting from genetically determined serumcholinesterase variants].", "content": "Prolonged apnea after succinyldicholin is a pharmacogenetic phenomenon. Estimation of genetically determined cholinesterase variants of patients with a prolonged apnea after succinyldicholin or in patients with cholinesterase deficiency respectively are described. The importance of atypical cholinesterase phenotypes in anaesthesia and the possibility of a therapy of prolonged apnea are mentioned. Biochemical data of atypical cholinesterase variants as cause of the prolonged degradation of succinyldicholin as well as formal genetic aspects of cholinesterase polymorphism are discussed.", "contents": "[Succinyldicholin sensitivity resulting from genetically determined serumcholinesterase variants]. Prolonged apnea after succinyldicholin is a pharmacogenetic phenomenon. Estimation of genetically determined cholinesterase variants of patients with a prolonged apnea after succinyldicholin or in patients with cholinesterase deficiency respectively are described. The importance of atypical cholinesterase phenotypes in anaesthesia and the possibility of a therapy of prolonged apnea are mentioned. Biochemical data of atypical cholinesterase variants as cause of the prolonged degradation of succinyldicholin as well as formal genetic aspects of cholinesterase polymorphism are discussed.", "PMID": 981143}
{"id": "pubmed23n0032_12560", "title": "[Damage of teeth by oral airways].", "content": "Oral Guedel airways do not necessarily protect the patient's teeth during inhalation anesthesia. This is contrary to the general opinion. Because of the anatomical structure of the human teeth and the dynamic forces on teeth occlusion during postanesthetic excitation the applied chewing-pressure is concentrated by a Guedel airway at a few front teeth only. Under this abnormally forceful, localized pressure especially capped teeth and teeth in gums with paradentosis may easily break. Therefore, the use of nasal airways should be preferred in these cases.", "contents": "[Damage of teeth by oral airways]. Oral Guedel airways do not necessarily protect the patient's teeth during inhalation anesthesia. This is contrary to the general opinion. Because of the anatomical structure of the human teeth and the dynamic forces on teeth occlusion during postanesthetic excitation the applied chewing-pressure is concentrated by a Guedel airway at a few front teeth only. Under this abnormally forceful, localized pressure especially capped teeth and teeth in gums with paradentosis may easily break. Therefore, the use of nasal airways should be preferred in these cases.", "PMID": 981144}
{"id": "pubmed23n0032_12561", "title": "[Simplified monitoring by ultrasound for anaesthesiology].", "content": "This is the description of a simplified method to assist the anaesthesiologist in monitoring and/or detecting venous and arterial diseases (i.e. stenosis) by ultrasound. The main problem for the anaesthesiologist is to monitor and measure the low blood pressure of new-borns during intra- and post-operative phases. In most cases it is difficult to localize the systolic pressure. We have developed a new device which, in connection with an analogic ratemeter, displays the heart rate based on the ultrasound doppler effect. Technique, application and first results are described.", "contents": "[Simplified monitoring by ultrasound for anaesthesiology]. This is the description of a simplified method to assist the anaesthesiologist in monitoring and/or detecting venous and arterial diseases (i.e. stenosis) by ultrasound. The main problem for the anaesthesiologist is to monitor and measure the low blood pressure of new-borns during intra- and post-operative phases. In most cases it is difficult to localize the systolic pressure. We have developed a new device which, in connection with an analogic ratemeter, displays the heart rate based on the ultrasound doppler effect. Technique, application and first results are described.", "PMID": 981145}
{"id": "pubmed23n0032_12562", "title": "[Rewarming of infusions with the haemotherm].", "content": "A rewarming device for blood called Haemotherm from the firm Bosch was proofed for efficiency. This device working on the base of microwaves was able to warm up cold solutions to a certain temperature within two to three minutes. The effect of rewarming disappeared during the passage through the infusion tube. To get bodywarm solutions at the end of the infusion tube was impossible with this arrangement.", "contents": "[Rewarming of infusions with the haemotherm]. A rewarming device for blood called Haemotherm from the firm Bosch was proofed for efficiency. This device working on the base of microwaves was able to warm up cold solutions to a certain temperature within two to three minutes. The effect of rewarming disappeared during the passage through the infusion tube. To get bodywarm solutions at the end of the infusion tube was impossible with this arrangement.", "PMID": 981146}
{"id": "pubmed23n0032_12563", "title": "[Use of pancreatic lipase for determination of the glyceride composition of rice lipids].", "content": "The triglyceride composition of rice lipids was investigated, using methods of thin-layer chromatography, enzymic hydrolysis by pancreatic lipase and gas-liquid chromatography. The major portion of triglycerides were tri- and di-unsaturated glycerides. The content of trisaturated glycerides was not more than 0.86%. The data obtained allow the conclusion that unsaturated acids occupy 2-positions in the triglyceride molecule of rice oil and oil of other plants.", "contents": "[Use of pancreatic lipase for determination of the glyceride composition of rice lipids]. The triglyceride composition of rice lipids was investigated, using methods of thin-layer chromatography, enzymic hydrolysis by pancreatic lipase and gas-liquid chromatography. The major portion of triglycerides were tri- and di-unsaturated glycerides. The content of trisaturated glycerides was not more than 0.86%. The data obtained allow the conclusion that unsaturated acids occupy 2-positions in the triglyceride molecule of rice oil and oil of other plants.", "PMID": 981202}
{"id": "pubmed23n0032_12564", "title": "[Microbiologic transformation of microcrystalline cortisone acetate into prednisone].", "content": "The process of transformation of microcystalline acetate cortisone by two mycobacteria (Mycobacterium album and Mycobacterium globiforme) has been studied. Optimal conditions for the process have been established: amount of biomass of both cultures, concentration of the initial substrate and required concentration of the surfactant--antifoamsilane. The anzymic activity of the cultures reaches maximum if the following three requirements are met: utilization of cells separated from the medium, substrate in the microcrystalline state and surfactant of the antifoamsilane type.", "contents": "[Microbiologic transformation of microcrystalline cortisone acetate into prednisone]. The process of transformation of microcystalline acetate cortisone by two mycobacteria (Mycobacterium album and Mycobacterium globiforme) has been studied. Optimal conditions for the process have been established: amount of biomass of both cultures, concentration of the initial substrate and required concentration of the surfactant--antifoamsilane. The anzymic activity of the cultures reaches maximum if the following three requirements are met: utilization of cells separated from the medium, substrate in the microcrystalline state and surfactant of the antifoamsilane type.", "PMID": 981200}
{"id": "pubmed23n0032_12565", "title": "[A quantitative method of determining lecithinases and lipases in microorganisms].", "content": "The paper presents a method for quantitative assay of lecithinases and lipases in microorganisms. The method is based on the assessment of changes in the composition of lipid components of yolk media under the influence of corresponding enzymic systems of bacteria. The technique involved lipid extraction from yolk media after cultivation of the microorganisms tested, chromatographic separation by thin-layer chromatography on silicagel into individual fractions, development in iodine vapours and quantitative measurement of lipid components.", "contents": "[A quantitative method of determining lecithinases and lipases in microorganisms]. The paper presents a method for quantitative assay of lecithinases and lipases in microorganisms. The method is based on the assessment of changes in the composition of lipid components of yolk media under the influence of corresponding enzymic systems of bacteria. The technique involved lipid extraction from yolk media after cultivation of the microorganisms tested, chromatographic separation by thin-layer chromatography on silicagel into individual fractions, development in iodine vapours and quantitative measurement of lipid components.", "PMID": 981203}
{"id": "pubmed23n0032_12566", "title": "New developments in ultrasonic fish tracking.", "content": "A range of small, long-life ultrasonic fish tags is described and their use illustrated with an improved system of fixed hydrophone tracking stations. A boat-mounted hydrophone system giving bearing and range estimations for use in open-water fish tracking is described together with a portable hand-held unit for simple location of tagged fish in rivers, etc. Typical results based on intensive use of these systems are given.", "contents": "New developments in ultrasonic fish tracking. A range of small, long-life ultrasonic fish tags is described and their use illustrated with an improved system of fixed hydrophone tracking stations. A boat-mounted hydrophone system giving bearing and range estimations for use in open-water fish tracking is described together with a portable hand-held unit for simple location of tagged fish in rivers, etc. Typical results based on intensive use of these systems are given.", "PMID": 981239}
{"id": "pubmed23n0032_12567", "title": "Production of larval tanypodinae (Insecta: Chironomidae) in the mud at Loch Leven, Kinross.", "content": "Life histories and production of the five common species of Tanypodinae (Pentaneura monilis, Procladius simplicistilus, P. crassinervis, P. choreus and Psilotanypus rufovittatus) in the mud at Loch Leven were measured from March 1971 to March 1972. The methods used to identify each species of larva are described. All were univoltine except Pentaneura monilis which had two generations per year, but the seasonal patterns of the life cycles were different. The average density of third and fourth instar Tanypodinae was 3100/m2 and densities of individual species exceeded 2500/m2 for short periods in Procladius crassinervis and P. choreus. Net annual dry weight production by this subfamily was 2-6 g/m2 (57 KJ/m2), with individual species ranging from 1-1 g/m2 (P. crassinervis) to 0-16 g/m2 (Pentaneura monilis). These results are discussed briefly and compared with production estimates for other zoobenthos.", "contents": "Production of larval tanypodinae (Insecta: Chironomidae) in the mud at Loch Leven, Kinross. Life histories and production of the five common species of Tanypodinae (Pentaneura monilis, Procladius simplicistilus, P. crassinervis, P. choreus and Psilotanypus rufovittatus) in the mud at Loch Leven were measured from March 1971 to March 1972. The methods used to identify each species of larva are described. All were univoltine except Pentaneura monilis which had two generations per year, but the seasonal patterns of the life cycles were different. The average density of third and fourth instar Tanypodinae was 3100/m2 and densities of individual species exceeded 2500/m2 for short periods in Procladius crassinervis and P. choreus. Net annual dry weight production by this subfamily was 2-6 g/m2 (57 KJ/m2), with individual species ranging from 1-1 g/m2 (P. crassinervis) to 0-16 g/m2 (Pentaneura monilis). These results are discussed briefly and compared with production estimates for other zoobenthos.", "PMID": 981240}
{"id": "pubmed23n0032_12568", "title": "Rumen micro-organisms in red deer, hill sheep and reindeer in the scottish highlands.", "content": "The micro-organisms in rumen samples from Red deer, hill sheep and reindeer grazing their natural pastures in the Scottish Highlands were examined at different seasons over a number of years. The total counts of bacteria and protozoa varied with the seasons, and were lowest in winter when fermentative activity was also at its lowest. As is usual in roughage-fed ruminants, viable counts were only a very small proportion of the total counts. The reindeer rumens had the highest concentrations of bacteria. There were no consistent differences in the types of bacteria between seasons, but although there were no bacteria peculiar to any animal species the balance of predominant types varied between the species. The principal types of bacteria were similar to those found in domesticated ruminants. The numbers of protozoa in the Red deer were higher than those in the sheep, and the concentrations in reindeer were higher than those generally reported for domesticated cattle or sheep. A number of species of protozoa were found in each animal and the predominant species differed in the different animals.", "contents": "Rumen micro-organisms in red deer, hill sheep and reindeer in the scottish highlands. The micro-organisms in rumen samples from Red deer, hill sheep and reindeer grazing their natural pastures in the Scottish Highlands were examined at different seasons over a number of years. The total counts of bacteria and protozoa varied with the seasons, and were lowest in winter when fermentative activity was also at its lowest. As is usual in roughage-fed ruminants, viable counts were only a very small proportion of the total counts. The reindeer rumens had the highest concentrations of bacteria. There were no consistent differences in the types of bacteria between seasons, but although there were no bacteria peculiar to any animal species the balance of predominant types varied between the species. The principal types of bacteria were similar to those found in domesticated ruminants. The numbers of protozoa in the Red deer were higher than those in the sheep, and the concentrations in reindeer were higher than those generally reported for domesticated cattle or sheep. A number of species of protozoa were found in each animal and the predominant species differed in the different animals.", "PMID": 981241}
{"id": "pubmed23n0032_12569", "title": "Simultaneous bilateral pyeloplasties.", "content": "A technique is described which gives excellent results in the treatment of unilateral hydronephorsis and allows for simultaneous bilateral pyeloplasties. The advantages for the patients with bilateral hydronephrosis are a single admission to hospital and a single anaesthetic and operative procedure. The exposure is superior to that achieved by a loin incision, allowing the surgeon to perform the pyeloplasties more rapidly and with less traction on the renal vessels. Over a follow-up period of 1-7 years no subsequent pyeloplasty or nephrectomy has been required and all 11 patients are well and symptom free.", "contents": "Simultaneous bilateral pyeloplasties. A technique is described which gives excellent results in the treatment of unilateral hydronephorsis and allows for simultaneous bilateral pyeloplasties. The advantages for the patients with bilateral hydronephrosis are a single admission to hospital and a single anaesthetic and operative procedure. The exposure is superior to that achieved by a loin incision, allowing the surgeon to perform the pyeloplasties more rapidly and with less traction on the renal vessels. Over a follow-up period of 1-7 years no subsequent pyeloplasty or nephrectomy has been required and all 11 patients are well and symptom free.", "PMID": 981265}
{"id": "pubmed23n0032_12570", "title": "Cross-tolerance between inhaled cannabis and intraperitoneal injections of delta9-THC.", "content": "Male and female rats were exposed to Cannabis smoke or placebo once every second day for 32 days. Following these 16 trials all animals were injected once intraperitoneally with 4 mg/kg THC. After every third inhalation trial and after the injection the rats were placed on a movement sensor for 3 min. Cannabis smoke significantly reduced activity, relative to baseline scores, during the first 10 inhalation trials but by the thirteenth exposure, tolerance was evident. When the animals were injected with THC, the male rats who had been exposed to Cannabis smoke significantly increased their activity whereas the females did not alter their activity relative to the last inhalation trial. In contrast rats of both sexes that had been exposed to placebo smoke significantly decreased their activity following the injection. This intermodal cross-tolerance is discussed in terms of the role of conditioning in the development of tolerance.", "contents": "Cross-tolerance between inhaled cannabis and intraperitoneal injections of delta9-THC. Male and female rats were exposed to Cannabis smoke or placebo once every second day for 32 days. Following these 16 trials all animals were injected once intraperitoneally with 4 mg/kg THC. After every third inhalation trial and after the injection the rats were placed on a movement sensor for 3 min. Cannabis smoke significantly reduced activity, relative to baseline scores, during the first 10 inhalation trials but by the thirteenth exposure, tolerance was evident. When the animals were injected with THC, the male rats who had been exposed to Cannabis smoke significantly increased their activity whereas the females did not alter their activity relative to the last inhalation trial. In contrast rats of both sexes that had been exposed to placebo smoke significantly decreased their activity following the injection. This intermodal cross-tolerance is discussed in terms of the role of conditioning in the development of tolerance.", "PMID": 981280}
{"id": "pubmed23n0032_12571", "title": "Action of di-n-propylacetate on the spontaneous and acquired behaviour in goldfish, mice and rats.", "content": "The action of n-dipropylacetate (nDPA) is related to the dose. In low doses, nDPA improved the number of conditioned responses with negative reinforcement. In higher doses, the drug inhibited spontaneous  and conditioned behaviour. An nDPA produces an increase in gamma-aminobutyric acid (GABA) brain level, results are discussed in relation to the inhibitory action of GABA in the central nervous system.", "contents": "Action of di-n-propylacetate on the spontaneous and acquired behaviour in goldfish, mice and rats. The action of n-dipropylacetate (nDPA) is related to the dose. In low doses, nDPA improved the number of conditioned responses with negative reinforcement. In higher doses, the drug inhibited spontaneous  and conditioned behaviour. An nDPA produces an increase in gamma-aminobutyric acid (GABA) brain level, results are discussed in relation to the inhibitory action of GABA in the central nervous system.", "PMID": 981281}
{"id": "pubmed23n0032_12572", "title": "Anteroventral third ventricle site of action for angiotensin induced thirst.", "content": "The central site of action for angiotensin induced thirst was investigated in rats. Subfornical organ lesions resulted in a temporary abolition of drinking induced by lateral preoptic or lateral ventricle microinjections of angiotensin but drinking to anteroventral third ventricle microinjections of angiotensin (or carbachol) was unaffected. Drinking to elevated systemic levels of angiotensin was attenuated but not abolished by subfornical organ lesions. When spread of injected angiotensin via cerebrospinal fluid circulation was controlled by placing plugs at selected locations in the ventricles, drinking was elicited only when intracranial microinjections of angiotensin gained access to anteroventral third ventricle. It was concluded that subfornical organ is not the exclusive dipsogenic receptor for angiotensin, rather angiotensin exerts at least part of its dipsogenic effect by spread through the ventricular system to receptors in the vicinity of the anteroventral third ventricle.", "contents": "Anteroventral third ventricle site of action for angiotensin induced thirst. The central site of action for angiotensin induced thirst was investigated in rats. Subfornical organ lesions resulted in a temporary abolition of drinking induced by lateral preoptic or lateral ventricle microinjections of angiotensin but drinking to anteroventral third ventricle microinjections of angiotensin (or carbachol) was unaffected. Drinking to elevated systemic levels of angiotensin was attenuated but not abolished by subfornical organ lesions. When spread of injected angiotensin via cerebrospinal fluid circulation was controlled by placing plugs at selected locations in the ventricles, drinking was elicited only when intracranial microinjections of angiotensin gained access to anteroventral third ventricle. It was concluded that subfornical organ is not the exclusive dipsogenic receptor for angiotensin, rather angiotensin exerts at least part of its dipsogenic effect by spread through the ventricular system to receptors in the vicinity of the anteroventral third ventricle.", "PMID": 981282}
{"id": "pubmed23n0032_12573", "title": "The relative importance of dopamine and noradrenaline receptor stimulation for the restoration of motor activity in reserpine or alpha-methyl-p-tyrosine pre-treated mice.", "content": "Two animal models of Parkinsonism have been employed to investigate the role of noradrenaline in the motor effects of levodopa. Pretreatment with reserpine or alpha-methyl-p-tyrosine (AMPT) causes cerebral amine depletion and reduction of motor activity, which can be reversed by levodopa. The effect of inhibitors of noradrenaline (NA) synthesis and antagonists of NA and dopamine (DA) receptors on the action of levodopa have been studied. For comparison, the effects of such treatments on apomorphine action has been investigated. Reversal of reserpine (10 mg/kg) induced akinesia in mice by levodopa (200 mg/kg) plus the peripheral decarboxylase inhibitor MK 486 (L-alpha-methyl-dopahydrazine; 25 mg/kg) was inhibited by prior administration of phenoxybenzamine (20 mg/kg), haloperidol (1 mg/kg), pimozide (1 mg/kg) or the dopamine-beta-hydroxylase inhibitor FLA-63 (bis [4-methyl-l-homopiperazinylthiocarbonyl] disulphide; 15 or 25 mg/kg). Apomorphine (2 mg/kg) reversal of reserpine akinesia was similarly inhibited by haloperidol (1 mg/kg) and pimozide (2 mg/kg) but not by phenoxybenzamine (20 mg/kg) or FLA-63 (25 mg/kg). Apomorphine (5 mg/kg) reversal of reserpine akinesia was enhanced by simultaneous administration of the noradrenergic agonist clonidine (1 mg/kg) and this effect was not significantly altered by prior administration of FLA-63. Clonidine, however, reversed the FLA-63 induced inhibition of the levodopa effect on reserpine akinesia. Levodopa reversal of akinesia induced by AMPT (200 mg/kg) was also inhibited by FLA-63, pimozide and haloperidol. Phenoxybenzamine, however, was without effect, but produced a different pattern of behaviour. Similarly, pimozide and haloperidol blocked apomorphine reversal of AMPT induced akinesia; FLA-63 was without effect but phenoxybenzamine produced marked inhibition. The results suggest that full restoration of motor activity in reserpine or AMPT pretreated animals requires stimulation of both DA and NA receptors.", "contents": "The relative importance of dopamine and noradrenaline receptor stimulation for the restoration of motor activity in reserpine or alpha-methyl-p-tyrosine pre-treated mice. Two animal models of Parkinsonism have been employed to investigate the role of noradrenaline in the motor effects of levodopa. Pretreatment with reserpine or alpha-methyl-p-tyrosine (AMPT) causes cerebral amine depletion and reduction of motor activity, which can be reversed by levodopa. The effect of inhibitors of noradrenaline (NA) synthesis and antagonists of NA and dopamine (DA) receptors on the action of levodopa have been studied. For comparison, the effects of such treatments on apomorphine action has been investigated. Reversal of reserpine (10 mg/kg) induced akinesia in mice by levodopa (200 mg/kg) plus the peripheral decarboxylase inhibitor MK 486 (L-alpha-methyl-dopahydrazine; 25 mg/kg) was inhibited by prior administration of phenoxybenzamine (20 mg/kg), haloperidol (1 mg/kg), pimozide (1 mg/kg) or the dopamine-beta-hydroxylase inhibitor FLA-63 (bis [4-methyl-l-homopiperazinylthiocarbonyl] disulphide; 15 or 25 mg/kg). Apomorphine (2 mg/kg) reversal of reserpine akinesia was similarly inhibited by haloperidol (1 mg/kg) and pimozide (2 mg/kg) but not by phenoxybenzamine (20 mg/kg) or FLA-63 (25 mg/kg). Apomorphine (5 mg/kg) reversal of reserpine akinesia was enhanced by simultaneous administration of the noradrenergic agonist clonidine (1 mg/kg) and this effect was not significantly altered by prior administration of FLA-63. Clonidine, however, reversed the FLA-63 induced inhibition of the levodopa effect on reserpine akinesia. Levodopa reversal of akinesia induced by AMPT (200 mg/kg) was also inhibited by FLA-63, pimozide and haloperidol. Phenoxybenzamine, however, was without effect, but produced a different pattern of behaviour. Similarly, pimozide and haloperidol blocked apomorphine reversal of AMPT induced akinesia; FLA-63 was without effect but phenoxybenzamine produced marked inhibition. The results suggest that full restoration of motor activity in reserpine or AMPT pretreated animals requires stimulation of both DA and NA receptors.", "PMID": 981283}
{"id": "pubmed23n0032_12574", "title": "Effect of hypnotics on mice genetically selected for sensitivity to ethanol.", "content": "It was previously shown that the rate of disappearance of blood ethanol was identical for two lines of mice selectively bred for differences in sleep-time after ethanol administration. The ED50 values for the loss of righting response with ethanol were significantly different at 3.64 g per kg for the SS line and 1.65 g per kg for the LS line. In the present study the mean sleep time is 367 sec for SS mice and 9342 sec for LS mice. The ED50 values remain essentially the same as previously reported. Unchanged LD50 values for ethanol, however, are not different at 4.8 g per kg for the SS and 4.5 g per kg for the LS line of mice. The ED50 value for loss for righting response following administration of methanol, butanol and t-butanol is approximately 2 fold greater for the SS line of mice than for the LS line. The ED50 values for sodium pentobarbital or ether in the 2 lines of mice for loss of righting response are virtually identical. In addition, the sleep-time values obtained after the administration of pentobarbital, chloral hydrate, trichloroethanol and paraldehyde are not significantly different. These data indicate that while the SS and LS lines of mice differ in central nervous system sensitivity to ethanol, methanol, butanol and t-butanol it is implied that they do no differ in central nervous system sensitivity to other hypnotic agents tested. Proof of this latter suggestion awaits determination of metabolic rates, and brain levels of these other depressants.", "contents": "Effect of hypnotics on mice genetically selected for sensitivity to ethanol. It was previously shown that the rate of disappearance of blood ethanol was identical for two lines of mice selectively bred for differences in sleep-time after ethanol administration. The ED50 values for the loss of righting response with ethanol were significantly different at 3.64 g per kg for the SS line and 1.65 g per kg for the LS line. In the present study the mean sleep time is 367 sec for SS mice and 9342 sec for LS mice. The ED50 values remain essentially the same as previously reported. Unchanged LD50 values for ethanol, however, are not different at 4.8 g per kg for the SS and 4.5 g per kg for the LS line of mice. The ED50 value for loss for righting response following administration of methanol, butanol and t-butanol is approximately 2 fold greater for the SS line of mice than for the LS line. The ED50 values for sodium pentobarbital or ether in the 2 lines of mice for loss of righting response are virtually identical. In addition, the sleep-time values obtained after the administration of pentobarbital, chloral hydrate, trichloroethanol and paraldehyde are not significantly different. These data indicate that while the SS and LS lines of mice differ in central nervous system sensitivity to ethanol, methanol, butanol and t-butanol it is implied that they do no differ in central nervous system sensitivity to other hypnotic agents tested. Proof of this latter suggestion awaits determination of metabolic rates, and brain levels of these other depressants.", "PMID": 981284}
{"id": "pubmed23n0032_12575", "title": "Intracerebral implantation of the anti-estrogen CN-69, 725-27: Effects on female sexual behavior in rats.", "content": "Ovariectomized adult female rats were stereotaxically implanted with double walled cannulas (22 ga outer and 27 ga inner) and tested for sexual receptivity (lordosis behavior) following SC estradiol benzoate and progesterone priming. Implantation of the anti-estrogen CN-69,725-27 (c. 65 mug) immediately after the first of 3 daily estrogen injections produced a dramatic inhibition of sexual receptivity when these implants were placed in the preoptic and anterior hypothalamic areas. CN-69,725-27 implants had no effect on receptivity when implanted in the middle hypothalamus, mesencephalon or frontal cortex. All inhibitory effects of the anti-estrogen were reversible within one day after removal of the CN-69,725-27 cannula from the brain.", "contents": "Intracerebral implantation of the anti-estrogen CN-69, 725-27: Effects on female sexual behavior in rats. Ovariectomized adult female rats were stereotaxically implanted with double walled cannulas (22 ga outer and 27 ga inner) and tested for sexual receptivity (lordosis behavior) following SC estradiol benzoate and progesterone priming. Implantation of the anti-estrogen CN-69,725-27 (c. 65 mug) immediately after the first of 3 daily estrogen injections produced a dramatic inhibition of sexual receptivity when these implants were placed in the preoptic and anterior hypothalamic areas. CN-69,725-27 implants had no effect on receptivity when implanted in the middle hypothalamus, mesencephalon or frontal cortex. All inhibitory effects of the anti-estrogen were reversible within one day after removal of the CN-69,725-27 cannula from the brain.", "PMID": 981285}
{"id": "pubmed23n0032_12576", "title": "Amino acid transport inhibition: brain and behavioral correlates.", "content": "In vivo inhibition of uptake 14C-L-valine by brain following subcutaneous administration of either of two gamma-glutamyl cycle enzyme inhibitors, 2-imidazolidone-4-carboxylic acid (ICA), or, L-methionine-S-sulfoximine (MSO) is documented in C57BL/6J mice. Dose related decrease in exploratory activity, impairment of memory for foot shock, and reduced operant responding for food reinforcement parallels the time course for interference with uptake of a large neutral amino acid by these two compounds previously shown to inhibit different enzymes in the gamma-glutamyl cycle subserving active amino acid transport.", "contents": "Amino acid transport inhibition: brain and behavioral correlates. In vivo inhibition of uptake 14C-L-valine by brain following subcutaneous administration of either of two gamma-glutamyl cycle enzyme inhibitors, 2-imidazolidone-4-carboxylic acid (ICA), or, L-methionine-S-sulfoximine (MSO) is documented in C57BL/6J mice. Dose related decrease in exploratory activity, impairment of memory for foot shock, and reduced operant responding for food reinforcement parallels the time course for interference with uptake of a large neutral amino acid by these two compounds previously shown to inhibit different enzymes in the gamma-glutamyl cycle subserving active amino acid transport.", "PMID": 981286}
{"id": "pubmed23n0032_12577", "title": "Are central cholinergic paths involved in habituation of exploration and distraction?", "content": "Experiment 1 tested the generality of Carlton's hypothesis that central muscarinic cholinergic pathways are involved in habituation of exploration. The effects of 3 muscarinic antagonists were tested in a holeboard, under 2 test conditions, i.e. with objects absent or present. Both the frequency and the duration of head-dipping were used as measures of exploration. Scopolamine prevented habituation only of the frequency of head-dipping, and only when objects were present. Atropine and benzhexol did not impair the habituation of either frequency of duration of head-dipping in either test condition. The impairment of habituation seemed therefore to be specific to scopolamine, and to the more complex test condition, and thus there was little to justify the suggestion that central cholinergic paths were generally involved. Experiment 2 investigated the effects of muscarinic antagonists on habituation of distraction. None of the drugs affected the distraction to tones, nor the subsequent habituation to these stimuli. Central cholinergic paths do not therefore seem to be involved in habituation of this behavioral response.", "contents": "Are central cholinergic paths involved in habituation of exploration and distraction? Experiment 1 tested the generality of Carlton's hypothesis that central muscarinic cholinergic pathways are involved in habituation of exploration. The effects of 3 muscarinic antagonists were tested in a holeboard, under 2 test conditions, i.e. with objects absent or present. Both the frequency and the duration of head-dipping were used as measures of exploration. Scopolamine prevented habituation only of the frequency of head-dipping, and only when objects were present. Atropine and benzhexol did not impair the habituation of either frequency of duration of head-dipping in either test condition. The impairment of habituation seemed therefore to be specific to scopolamine, and to the more complex test condition, and thus there was little to justify the suggestion that central cholinergic paths were generally involved. Experiment 2 investigated the effects of muscarinic antagonists on habituation of distraction. None of the drugs affected the distraction to tones, nor the subsequent habituation to these stimuli. Central cholinergic paths do not therefore seem to be involved in habituation of this behavioral response.", "PMID": 981287}
{"id": "pubmed23n0032_12578", "title": "Automated system for acquisition and reduction of startle response data.", "content": "A system specifically designed for the acquisition and reduction of startle data is described. It is able to (1) sample startle responses from 5 animals simultaneously during a specific time band after the eliciting stimulus; (2) convert the analogue startle amplitudes into 2-digit numbers; (3) print the digital results of each startle in each animal; (4) add up the startle amplitudes for each rat over a preset number of stimuli and print the totals; (5) print the interstimulus interval and (6) code for up to six diferent types of trials. A non-technical description and complete wiring diagrams are provided.", "contents": "Automated system for acquisition and reduction of startle response data. A system specifically designed for the acquisition and reduction of startle data is described. It is able to (1) sample startle responses from 5 animals simultaneously during a specific time band after the eliciting stimulus; (2) convert the analogue startle amplitudes into 2-digit numbers; (3) print the digital results of each startle in each animal; (4) add up the startle amplitudes for each rat over a preset number of stimuli and print the totals; (5) print the interstimulus interval and (6) code for up to six diferent types of trials. A non-technical description and complete wiring diagrams are provided.", "PMID": 981288}
{"id": "pubmed23n0032_12579", "title": "A simple method for quantifying tremor in rodents.", "content": "A simple and accurate device to evaluate frequency and intensity of involuntary tremor is described. Discrete and quantifiable measures of tremor could be obtained in terms of vertical (i.e., absolute effects at a given time) and horizontal (i.e. temporal) changes. The technique was evaluated employing dosages of physostigmine 0.1-0.7 mg/kg. Applications to other behavioral indices, such as locomotor activity and wet-dog shakes, are discussed.", "contents": "A simple method for quantifying tremor in rodents. A simple and accurate device to evaluate frequency and intensity of involuntary tremor is described. Discrete and quantifiable measures of tremor could be obtained in terms of vertical (i.e., absolute effects at a given time) and horizontal (i.e. temporal) changes. The technique was evaluated employing dosages of physostigmine 0.1-0.7 mg/kg. Applications to other behavioral indices, such as locomotor activity and wet-dog shakes, are discussed.", "PMID": 981289}
{"id": "pubmed23n0032_12580", "title": "Time dependent action of pimozide on deprivation-induced water intake: evidence for a direct drug effect.", "content": "Time-response curves were established for the effect of 3 doses of pimozide, a potent and specific dopamine receptor blocker, on deprivation-induced drinking in rats. Injections occurred once every 3 days at intervals of 2, 4, 8, and 16 hr prior to the daily 15 min drinking period. Pimozide produced a significant attenuation in the amount of water normally consumed (on nondrug days) whose degree varied with the proximity of the injection to the drinking period. Only the 2 higher doses (0.5 and 1.0 mg/kg) produced a significant dose dependent effect which was strongest at the 2 hr interval and which gradually weakened by the 8th post injection hr. The relevance of these findings for a direct versus an indirect action of the drug on water intake is discussed.", "contents": "Time dependent action of pimozide on deprivation-induced water intake: evidence for a direct drug effect. Time-response curves were established for the effect of 3 doses of pimozide, a potent and specific dopamine receptor blocker, on deprivation-induced drinking in rats. Injections occurred once every 3 days at intervals of 2, 4, 8, and 16 hr prior to the daily 15 min drinking period. Pimozide produced a significant attenuation in the amount of water normally consumed (on nondrug days) whose degree varied with the proximity of the injection to the drinking period. Only the 2 higher doses (0.5 and 1.0 mg/kg) produced a significant dose dependent effect which was strongest at the 2 hr interval and which gradually weakened by the 8th post injection hr. The relevance of these findings for a direct versus an indirect action of the drug on water intake is discussed.", "PMID": 981290}
{"id": "pubmed23n0032_12581", "title": "Noise stress and the effects of viloxazine (Vivalan), a new antidepressant, on open field activity in rats.", "content": "The effects of different types and levels of background noise on the response to Viloxazine (Vivalan) were studied in rats. The results showed that increasing the level of noise produced changes in activity which were dependent on its mode of presentation (type). The drug appeared to enhance these changes.", "contents": "Noise stress and the effects of viloxazine (Vivalan), a new antidepressant, on open field activity in rats. The effects of different types and levels of background noise on the response to Viloxazine (Vivalan) were studied in rats. The results showed that increasing the level of noise produced changes in activity which were dependent on its mode of presentation (type). The drug appeared to enhance these changes.", "PMID": 981291}
{"id": "pubmed23n0032_12582", "title": "Repeated acquisition of behavioral chains: effects of methylphenidate and imipramine.", "content": "A method involving repeated acquistion of behavioral chain was used to assess the effects of methylphenidate and imipramine in individual animals. Pigeons obtained food for completing a 4-response chain, which was changed from session to session. Learning was defined by the decrease in errors across trials within a session; overall accuracy was measured by total errors per session. For comparison, the drug tests were also conducted under a performance condition, in which the 4-response chain was the same from session to session. In general, both drugs increased total errors per session as a function of dose under both the learning and performance conditions. The error-increasing effect was greater with imipramine than with methylphenidate and was detected at lower doses under the learning condition than under the performance condition. Under the learning condition, the higher doses of both drugs decreased the rate of within-session error reduction. Although neither drug enhanced accuracy at any of the doses tested, the lower doses of methylphenidate slightly decreased total trial time under both the learning and performance conditions.", "contents": "Repeated acquisition of behavioral chains: effects of methylphenidate and imipramine. A method involving repeated acquistion of behavioral chain was used to assess the effects of methylphenidate and imipramine in individual animals. Pigeons obtained food for completing a 4-response chain, which was changed from session to session. Learning was defined by the decrease in errors across trials within a session; overall accuracy was measured by total errors per session. For comparison, the drug tests were also conducted under a performance condition, in which the 4-response chain was the same from session to session. In general, both drugs increased total errors per session as a function of dose under both the learning and performance conditions. The error-increasing effect was greater with imipramine than with methylphenidate and was detected at lower doses under the learning condition than under the performance condition. Under the learning condition, the higher doses of both drugs decreased the rate of within-session error reduction. Although neither drug enhanced accuracy at any of the doses tested, the lower doses of methylphenidate slightly decreased total trial time under both the learning and performance conditions.", "PMID": 981292}
{"id": "pubmed23n0032_12583", "title": "Synthesis of some phthalazine derivatives chemically related to hydralazine.", "content": "The potent antihypertensive properties of hydralazine and dihydralazine prompted the synthesis of sixteen new phthalazine derivatives with thiosemicarbazide or beta-propionamide residues to investigate their possible antihypertensive activity.", "contents": "Synthesis of some phthalazine derivatives chemically related to hydralazine. The potent antihypertensive properties of hydralazine and dihydralazine prompted the synthesis of sixteen new phthalazine derivatives with thiosemicarbazide or beta-propionamide residues to investigate their possible antihypertensive activity.", "PMID": 981294}
{"id": "pubmed23n0032_12584", "title": "Synthesis of certain spiro compounds.", "content": "The synthesis of certain N-substituted azaspirodiones and azaspiranes is described. Fusing equimolecular amounts of 2-oxaspiro [4.4]nonane-1.3-dione with a number of amino compounds afforded the corresponding N-substituted azaspirodiones. However, with certain o-substituted anilines, no condensation took place. Reduction of the N-haloaryl azaspirodiones gave the corresponding oxygen free compounds. Other azaspiranes were isolated as the quaternary methiodides. Applying the Mannich conditions to 2-azaspiro[4.4]nonane-1.3-dione yielded the N-Mannich bases.", "contents": "Synthesis of certain spiro compounds. The synthesis of certain N-substituted azaspirodiones and azaspiranes is described. Fusing equimolecular amounts of 2-oxaspiro [4.4]nonane-1.3-dione with a number of amino compounds afforded the corresponding N-substituted azaspirodiones. However, with certain o-substituted anilines, no condensation took place. Reduction of the N-haloaryl azaspirodiones gave the corresponding oxygen free compounds. Other azaspiranes were isolated as the quaternary methiodides. Applying the Mannich conditions to 2-azaspiro[4.4]nonane-1.3-dione yielded the N-Mannich bases.", "PMID": 981296}
{"id": "pubmed23n0032_12585", "title": "Argentometric titration of procaine hydrochloride with zero current bipotentiometric indication using pretreated silver electrodes.", "content": "A new procedure for the quantitative estimation of procaine hydrochloride is given based on the argentometric titration of its hydrochloride content, and using a zero current bipotentiometric indication. For this purpose two differently prepared silver electrodes are used. Quantitative data concerning precision are also given.", "contents": "Argentometric titration of procaine hydrochloride with zero current bipotentiometric indication using pretreated silver electrodes. A new procedure for the quantitative estimation of procaine hydrochloride is given based on the argentometric titration of its hydrochloride content, and using a zero current bipotentiometric indication. For this purpose two differently prepared silver electrodes are used. Quantitative data concerning precision are also given.", "PMID": 981298}
{"id": "pubmed23n0032_12586", "title": "The in-vitro adsorption of some antirheumatics on antacids.", "content": "The adsorption of sodium salicylate, salicylamide, acetylsalicylic acid, paracetamol, mefenamic acid, flufenamic acid, phenylbutazone, oxyphenbutazone, phenazone, aminophenazone, indometacin and methiazinic acid on some antacids was studied. The antacids used were magnesium trisilicate, magnesium oxide, aluminium hydroxide, bismuth oxycarbonate, calcium carbonate and kaolin. Magnesium oxide, followed by aluminium hydroxide and bismuth oxycarbonate showed a fairly high adsorptive capacity for salicylates, mefenamic acid, flufenamic acid, methiazinic acid, indometacin and to a lesser extent for phenylbutazone and oxyphenbutazone. On the other hand, magnesium trisilicate exhibited a tendency to adsorb phenazone, aminophenazone, indometacin and methiazinic acid. Kaolin was found to be a good adsorbent for anthranilic acid derivatives, indometacin and methiazinic acid. Calcium carbonate showed a weak adsorptive capacity for all drugs tested. The adsorption of phenylbutazone and salicylates on magnesium oxide, aluminium hydroxide and/or bismuth oxycarbonate obeyed the Freundlich adsorption isotherm. Elution study showed that salicylates and anthranilic acid derivatives were tenaciously held by magnesium oxide while magnesium trisilicate showed an intermediate retention power for phenazone and aminophenazone. Sodium hydrogen carbonate solution gave, in general, a higher eluting power than hydrochloric acid solution. A marked reduction in the apparent partition coefficients of all drugs tested was observed in the presence of magnesium trisilicate or aluminium hydroxide. Careful in vitro and in vivo testing of drug availability is advisable prior to the concomitant administration of antirheumatics with antacids or other adsorbents.", "contents": "The in-vitro adsorption of some antirheumatics on antacids. The adsorption of sodium salicylate, salicylamide, acetylsalicylic acid, paracetamol, mefenamic acid, flufenamic acid, phenylbutazone, oxyphenbutazone, phenazone, aminophenazone, indometacin and methiazinic acid on some antacids was studied. The antacids used were magnesium trisilicate, magnesium oxide, aluminium hydroxide, bismuth oxycarbonate, calcium carbonate and kaolin. Magnesium oxide, followed by aluminium hydroxide and bismuth oxycarbonate showed a fairly high adsorptive capacity for salicylates, mefenamic acid, flufenamic acid, methiazinic acid, indometacin and to a lesser extent for phenylbutazone and oxyphenbutazone. On the other hand, magnesium trisilicate exhibited a tendency to adsorb phenazone, aminophenazone, indometacin and methiazinic acid. Kaolin was found to be a good adsorbent for anthranilic acid derivatives, indometacin and methiazinic acid. Calcium carbonate showed a weak adsorptive capacity for all drugs tested. The adsorption of phenylbutazone and salicylates on magnesium oxide, aluminium hydroxide and/or bismuth oxycarbonate obeyed the Freundlich adsorption isotherm. Elution study showed that salicylates and anthranilic acid derivatives were tenaciously held by magnesium oxide while magnesium trisilicate showed an intermediate retention power for phenazone and aminophenazone. Sodium hydrogen carbonate solution gave, in general, a higher eluting power than hydrochloric acid solution. A marked reduction in the apparent partition coefficients of all drugs tested was observed in the presence of magnesium trisilicate or aluminium hydroxide. Careful in vitro and in vivo testing of drug availability is advisable prior to the concomitant administration of antirheumatics with antacids or other adsorbents.", "PMID": 981301}
{"id": "pubmed23n0032_12587", "title": "Neuroendocrine studies of depressive illness.", "content": "Interest in possible neuroendocrine disturbances in endogenous depression is prompted by two lines of evidence: (1) clinical features of the illness suggest hypothalamic dysfunction; (2) the brain neurotransmitters implicated in depression also regulate neuroendocrine function. Our research reveals a marked, sustained hypersecretion in cortisol in severe depressive illness, which is apparently unrelated to stress and sleep disturbance, and which is associated with a distortion of the 24-hour cortisol secretory pattern. The hypersecretion is manifested primarily in the late afternoon, evening, and early morning hours, when cortisol secretion is normally inhibited. Growth hormone responses to hypoglycemia (but not to L-dopa) are also significantly reduced in endogenous depression, even when factors of age and the menopause are controlled. Postmenopausal depressed women appear to secret significantly less LH than normal postmenopausal women. Since all of these hormonal abnormalities can be reproduced by depletion of brain noradrenalin, the findings provide support for the the hypothesis of reduced functional noradrenergic activity in certain forms of depression.", "contents": "Neuroendocrine studies of depressive illness. Interest in possible neuroendocrine disturbances in endogenous depression is prompted by two lines of evidence: (1) clinical features of the illness suggest hypothalamic dysfunction; (2) the brain neurotransmitters implicated in depression also regulate neuroendocrine function. Our research reveals a marked, sustained hypersecretion in cortisol in severe depressive illness, which is apparently unrelated to stress and sleep disturbance, and which is associated with a distortion of the 24-hour cortisol secretory pattern. The hypersecretion is manifested primarily in the late afternoon, evening, and early morning hours, when cortisol secretion is normally inhibited. Growth hormone responses to hypoglycemia (but not to L-dopa) are also significantly reduced in endogenous depression, even when factors of age and the menopause are controlled. Postmenopausal depressed women appear to secret significantly less LH than normal postmenopausal women. Since all of these hormonal abnormalities can be reproduced by depletion of brain noradrenalin, the findings provide support for the the hypothesis of reduced functional noradrenergic activity in certain forms of depression.", "PMID": 981319}
{"id": "pubmed23n0032_12588", "title": "Clinical and biochemical parameters during neuroleptic treatment. I. Investigations with haloperidol.", "content": "26 patients with a paranoid-hallucinatory syndrome were treated with a fixed daily dose of haloperidol. Psychopathological symptoms and parkinsonism were studied before and during the treatment. HVA and 5-HIAA concentrations in CSF were determined immediately before and after 5 or 15 days of treatment. After 5 days of treatment a slight effect was observed on psychotic symptoms and on the extrapyramidal motor system, as well as an elevation of HVA and 5-HIAA concentrations in CSF. After 15 days of treatment the extrapyramidal and antipsychotic effects were significant, but the rise of HVA was smaller than after 5 days of treatment; 5-HIAA remained unchanged. These different time courses of the neuroleptic effects on psychopathological symptoms, on extrapyramidal system and on monoamine metabolites concentration in CSF could be interpreted as a development of drug tolerance in relation to the biochemical parameters.", "contents": "Clinical and biochemical parameters during neuroleptic treatment. I. Investigations with haloperidol. 26 patients with a paranoid-hallucinatory syndrome were treated with a fixed daily dose of haloperidol. Psychopathological symptoms and parkinsonism were studied before and during the treatment. HVA and 5-HIAA concentrations in CSF were determined immediately before and after 5 or 15 days of treatment. After 5 days of treatment a slight effect was observed on psychotic symptoms and on the extrapyramidal motor system, as well as an elevation of HVA and 5-HIAA concentrations in CSF. After 15 days of treatment the extrapyramidal and antipsychotic effects were significant, but the rise of HVA was smaller than after 5 days of treatment; 5-HIAA remained unchanged. These different time courses of the neuroleptic effects on psychopathological symptoms, on extrapyramidal system and on monoamine metabolites concentration in CSF could be interpreted as a development of drug tolerance in relation to the biochemical parameters.", "PMID": 981320}
{"id": "pubmed23n0032_12589", "title": "Clinical and biochemical parameters during neuroleptic treatment. II. Antipsychotic effect of haloperidol in patients with chronic organic brain damage.", "content": "The time-course of psychopathological symptoms, of extrapyramidal side effects, and of changes in cerebrospinal fluid (CSF) concentration of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were simultaneously studied during Haloperidol treatment of 14 psychotic patients with chronic organic brain damage. After 15 days of treatment significant antipsychotic effect was found, while Parkinsonism scores in clinical and experimental tests increased only slightly. CSF concentration of HVA increased significantly by 150% compared to the baseline value (p less than 0.05) and 5-HIAA remained unchanged. No correlation was found between the clinical and biochemical variables studied. The comparison of these results with those obtained in patients without brain damage suggests that different psychopathological and extrapyramidal responses to neuroleptics are not strictly associated with specific HVA changes in CSF.", "contents": "Clinical and biochemical parameters during neuroleptic treatment. II. Antipsychotic effect of haloperidol in patients with chronic organic brain damage. The time-course of psychopathological symptoms, of extrapyramidal side effects, and of changes in cerebrospinal fluid (CSF) concentration of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were simultaneously studied during Haloperidol treatment of 14 psychotic patients with chronic organic brain damage. After 15 days of treatment significant antipsychotic effect was found, while Parkinsonism scores in clinical and experimental tests increased only slightly. CSF concentration of HVA increased significantly by 150% compared to the baseline value (p less than 0.05) and 5-HIAA remained unchanged. No correlation was found between the clinical and biochemical variables studied. The comparison of these results with those obtained in patients without brain damage suggests that different psychopathological and extrapyramidal responses to neuroleptics are not strictly associated with specific HVA changes in CSF.", "PMID": 981321}
{"id": "pubmed23n0032_12590", "title": "Clinical and biochemical parameters during neuroleptic treatment. III. Primitive reflexes during neuroleptic treatment.", "content": "Palmomental reflex, glabellar reflex and oral responses were studied in the course of a treatment with Haloperidol in 14 psychotic patients with a chronic organic brain damage. An increase of the three responses was found; maximum intensity and frequency of the primitive reflexes were reached during the first 5 days of treatment. There was no correlation found between the intensity of primitive reflexes and changes of psychopathological symptoms, of extrapyramidal scores and of homovanillic acid (=HVA) and 5-hydroxyindoleacetic acid (5=5-HIAA) concentration in cerebrospinal fluid (=CSF). The presence of primitive responses is interpreted as a \"decompensation phenomenon\" induced by neuroleptics, probably in relation to an effect on the central nervous system (=CNS) dopamine receptors. It is suggested that the appearance of several definite, primitive reflexes during neuroleptic therapy may have some diagnostic and prognostic significance.", "contents": "Clinical and biochemical parameters during neuroleptic treatment. III. Primitive reflexes during neuroleptic treatment. Palmomental reflex, glabellar reflex and oral responses were studied in the course of a treatment with Haloperidol in 14 psychotic patients with a chronic organic brain damage. An increase of the three responses was found; maximum intensity and frequency of the primitive reflexes were reached during the first 5 days of treatment. There was no correlation found between the intensity of primitive reflexes and changes of psychopathological symptoms, of extrapyramidal scores and of homovanillic acid (=HVA) and 5-hydroxyindoleacetic acid (5=5-HIAA) concentration in cerebrospinal fluid (=CSF). The presence of primitive responses is interpreted as a \"decompensation phenomenon\" induced by neuroleptics, probably in relation to an effect on the central nervous system (=CNS) dopamine receptors. It is suggested that the appearance of several definite, primitive reflexes during neuroleptic therapy may have some diagnostic and prognostic significance.", "PMID": 981322}
{"id": "pubmed23n0032_12591", "title": "Psychotropic drug use in family practice.", "content": "In an attempt to assess the precision of an illness-treatment \"fit\" between dispensing and actual need for psychotropic medications, the present study examined the dispensing patterns to consecutive attenders of private general practice. \"Conservative\" use of psychotropic drugs was indicated insofar as patients were seldom dispensed medication in the absence of emotional problems, and the presence of emotional problems frequently was not accompanied by the dispensing of medication. In addition, specific social and illness factors were located which could differentially predict either the presence of emotional problems, the use of psychotropic drugs, or both of these variables. The potential for constructing a more precise illness-treatment fit was considered.", "contents": "Psychotropic drug use in family practice. In an attempt to assess the precision of an illness-treatment \"fit\" between dispensing and actual need for psychotropic medications, the present study examined the dispensing patterns to consecutive attenders of private general practice. \"Conservative\" use of psychotropic drugs was indicated insofar as patients were seldom dispensed medication in the absence of emotional problems, and the presence of emotional problems frequently was not accompanied by the dispensing of medication. In addition, specific social and illness factors were located which could differentially predict either the presence of emotional problems, the use of psychotropic drugs, or both of these variables. The potential for constructing a more precise illness-treatment fit was considered.", "PMID": 981323}
{"id": "pubmed23n0032_12592", "title": "[A comparison of the tranquilizing effect of fluspirilene and diazepam (author's transl)].", "content": "Over a period of 8 weeks we compared the tranquilizing effects of the longterm neuroleptic Fluspirilene to those of Diazepam in a series of 28 patients. Both drugs showed good effects on somatization, anxiety and depressive mood. Fluspirilene, however, proved to be the more mood elevating and more activating agent without being hypnotic. The indications for both drugs are being discussed emphazising the new therapeutic perspectives of a long acting tranquilizer.", "contents": "[A comparison of the tranquilizing effect of fluspirilene and diazepam (author's transl)]. Over a period of 8 weeks we compared the tranquilizing effects of the longterm neuroleptic Fluspirilene to those of Diazepam in a series of 28 patients. Both drugs showed good effects on somatization, anxiety and depressive mood. Fluspirilene, however, proved to be the more mood elevating and more activating agent without being hypnotic. The indications for both drugs are being discussed emphazising the new therapeutic perspectives of a long acting tranquilizer.", "PMID": 981324}
{"id": "pubmed23n0032_12593", "title": "Evaluation of the lithium RBC/plasma ratio as a predictor of the prophylactic effect of lithium treatment in affective disorders.", "content": "62 patients with affective disorder, 31 unipolar, 22 bipolar and 9 cycloid psychotics who had received prophylactic lithium therapy for 0,3 to 7,5 years were studied. Lithium in plasma, lithium in red blood cells (RBC) and the lithium ratio (RBC/plasma) were estimated. The lithium ratio does not seem to be of predictive value in determining for which patients prophylactic lithium therapy will succeed.", "contents": "Evaluation of the lithium RBC/plasma ratio as a predictor of the prophylactic effect of lithium treatment in affective disorders. 62 patients with affective disorder, 31 unipolar, 22 bipolar and 9 cycloid psychotics who had received prophylactic lithium therapy for 0,3 to 7,5 years were studied. Lithium in plasma, lithium in red blood cells (RBC) and the lithium ratio (RBC/plasma) were estimated. The lithium ratio does not seem to be of predictive value in determining for which patients prophylactic lithium therapy will succeed.", "PMID": 981325}
{"id": "pubmed23n0032_12594", "title": "Lithium carbonate prophylaxis of depression in three subtypes in primary affective disorder.", "content": "Multiple indices of depression were used to evaluate the prophylactic efficacy of lithium carbonate versus placebo in a 4-year, double-blind study of unipolar, bipolar I, and bipolar II patients diagnosed according to strict criteria. Our data indicate lithium prophylaxis of depression on several indices in all three subtypes of affective illness. Additional studies are needed, comparing tricyclic antidepressants alone, monoamine oxidase inhibitors alone, or either drug combination with lithium carbonate for prophylaxis in clearly defined depressive subtypes.", "contents": "Lithium carbonate prophylaxis of depression in three subtypes in primary affective disorder. Multiple indices of depression were used to evaluate the prophylactic efficacy of lithium carbonate versus placebo in a 4-year, double-blind study of unipolar, bipolar I, and bipolar II patients diagnosed according to strict criteria. Our data indicate lithium prophylaxis of depression on several indices in all three subtypes of affective illness. Additional studies are needed, comparing tricyclic antidepressants alone, monoamine oxidase inhibitors alone, or either drug combination with lithium carbonate for prophylaxis in clearly defined depressive subtypes.", "PMID": 981326}
{"id": "pubmed23n0032_12595", "title": "Maintenance therapy of depression.", "content": "This paper summarises findings from a study of maintenance treatment in predominantly neurotic depressive responding to initial treatment with amitriptyline. Patients were continued on amitriptyline for eight months, withdrawn double-blind to placebo after two months, or withdrawn overtly to no medication, with and without individual psychotherapy from social workers in the factorial design. Continuation significantly reduced early relapse and symptom return, compared with either condition of early withdrawal. Psychotherapy improved social adjustment after eight months in patients who did not relapse, without any significant interactions. Side effects were minimal, except for carbohydrate craving. These findings suggest that tricyclic antidepressants should be routinely continued for several months after drug-induced remission.", "contents": "Maintenance therapy of depression. This paper summarises findings from a study of maintenance treatment in predominantly neurotic depressive responding to initial treatment with amitriptyline. Patients were continued on amitriptyline for eight months, withdrawn double-blind to placebo after two months, or withdrawn overtly to no medication, with and without individual psychotherapy from social workers in the factorial design. Continuation significantly reduced early relapse and symptom return, compared with either condition of early withdrawal. Psychotherapy improved social adjustment after eight months in patients who did not relapse, without any significant interactions. Side effects were minimal, except for carbohydrate craving. These findings suggest that tricyclic antidepressants should be routinely continued for several months after drug-induced remission.", "PMID": 981327}
{"id": "pubmed23n0032_12596", "title": "How long should prophylactics be continued in affective disorders?", "content": "The article traces the historical development of the prophylactic drug treatment of affective disorders. Prior to the introduction of lithium, standard anti-depressant drugs were used for maintenance treatment. Historically, lithium did not introduce prophylactic treatment. It did, however, dramatically advance the concept and administration of the prophylactic treatment of affective disorders. Specific criteria must now be developed to determine the duration of prophylactic treatment in terms of individual illness profiles. Therapeutic goals and clinical problems in the administration of treatment represent a main part of the paper.", "contents": "How long should prophylactics be continued in affective disorders? The article traces the historical development of the prophylactic drug treatment of affective disorders. Prior to the introduction of lithium, standard anti-depressant drugs were used for maintenance treatment. Historically, lithium did not introduce prophylactic treatment. It did, however, dramatically advance the concept and administration of the prophylactic treatment of affective disorders. Specific criteria must now be developed to determine the duration of prophylactic treatment in terms of individual illness profiles. Therapeutic goals and clinical problems in the administration of treatment represent a main part of the paper.", "PMID": 981328}
{"id": "pubmed23n0032_12597", "title": "Therapeutic control of plasma concentrations and long-term effect of nortriptyline in recurrent affective disorders.", "content": "Based on the evidence that therapeutic plasma concentration range in fact exists for the tricyclic antidepressant drug, Nortriptyline (range 50-150 ng/ml), three different investigations were under taken in order to clarify some clinical pharmacological problems during long-term treatment with this drug. The possible prophlactic effect of the drug in recurrent affective disorders was specially examined in a group of patients with a high risk of episodes in their unipolar manic-depressive disease. The results highly demonstrate the value of monitoring plasma levels in achieving therapeutic control. Depressive relapses during treatment, for months and years, were only related to therapeutic insufficient plasma levels of the drug.", "contents": "Therapeutic control of plasma concentrations and long-term effect of nortriptyline in recurrent affective disorders. Based on the evidence that therapeutic plasma concentration range in fact exists for the tricyclic antidepressant drug, Nortriptyline (range 50-150 ng/ml), three different investigations were under taken in order to clarify some clinical pharmacological problems during long-term treatment with this drug. The possible prophlactic effect of the drug in recurrent affective disorders was specially examined in a group of patients with a high risk of episodes in their unipolar manic-depressive disease. The results highly demonstrate the value of monitoring plasma levels in achieving therapeutic control. Depressive relapses during treatment, for months and years, were only related to therapeutic insufficient plasma levels of the drug.", "PMID": 981330}
{"id": "pubmed23n0032_12598", "title": "Effects of short-and long-term administration of tricyclic antidepressants and lithium on norepinephrine turnover in brain.", "content": "The findings summarized in this paper show that norepinephrine turnover in brain is decreased after acute administration of imipramine or desmethylimipramine but tends to increase during chronic administration of these tricyclic antidepressants. Similarly, it appears that there also may be important differences between the effects of acute and chronic administration of lithium salts on norepinephrine turnover in the central nervous system. Such changes in norepinephrine turnover that develop gradually over the course of long-term drug administration may help to explain the need for chronic administration of tricyclic antidepressants or lithium salts in the treatment of patients with affective disorders.", "contents": "Effects of short-and long-term administration of tricyclic antidepressants and lithium on norepinephrine turnover in brain. The findings summarized in this paper show that norepinephrine turnover in brain is decreased after acute administration of imipramine or desmethylimipramine but tends to increase during chronic administration of these tricyclic antidepressants. Similarly, it appears that there also may be important differences between the effects of acute and chronic administration of lithium salts on norepinephrine turnover in the central nervous system. Such changes in norepinephrine turnover that develop gradually over the course of long-term drug administration may help to explain the need for chronic administration of tricyclic antidepressants or lithium salts in the treatment of patients with affective disorders.", "PMID": 981331}
{"id": "pubmed23n0032_12599", "title": "Absence of correlation between spin-lattice relaxation times and water content in human tumor tissues.", "content": "With a view to ascertaining the possible role of water content of the cells in the reported elevated values for proton spin-lattice relaxation time (T1) in malignant tissues, measurements of percent water content as well as T1 were undertaken in 68 cases of normal and concerous tissues from diverse sites in human patients. T1 values for the uninvolved samples were found to lie in the range of 400-1000 msec while the corresponding range for the involved samples was from 450-1520 msec. Water content of both normal and uninvolved tissues mostly lay in the narrow range of 70-90%. Occasionally samples with large T1 values showed lower water content as well. Data obtained weakens the case for characterization of tumor cells on the basis of their water content alone.", "contents": "Absence of correlation between spin-lattice relaxation times and water content in human tumor tissues. With a view to ascertaining the possible role of water content of the cells in the reported elevated values for proton spin-lattice relaxation time (T1) in malignant tissues, measurements of percent water content as well as T1 were undertaken in 68 cases of normal and concerous tissues from diverse sites in human patients. T1 values for the uninvolved samples were found to lie in the range of 400-1000 msec while the corresponding range for the involved samples was from 450-1520 msec. Water content of both normal and uninvolved tissues mostly lay in the narrow range of 70-90%. Occasionally samples with large T1 values showed lower water content as well. Data obtained weakens the case for characterization of tumor cells on the basis of their water content alone.", "PMID": 981352}
{"id": "pubmed23n0032_12600", "title": "Diamagnetic levitation in the fractionally superconducting bile cholates.", "content": "Diamagnetic levitation similar to that seen in metallic superconductors has been observed in organic compounds; namely, the bile cholates. Levitation phenomena, with forces exceeding 400 lbs/in, occurred in repeated experiments at transition temperatures extablished by susceptibility and resistivity measurements. With crystalline phase change ruled out by X-ray diffraction studies, the observed levitation must be regarded as due to superconductive effects occurring in small domains randomly dispersed throughout the insulating bulk of the investigated cholates. The transition temperatures observed in some of these compounds were higher than those seen in the metals.", "contents": "Diamagnetic levitation in the fractionally superconducting bile cholates. Diamagnetic levitation similar to that seen in metallic superconductors has been observed in organic compounds; namely, the bile cholates. Levitation phenomena, with forces exceeding 400 lbs/in, occurred in repeated experiments at transition temperatures extablished by susceptibility and resistivity measurements. With crystalline phase change ruled out by X-ray diffraction studies, the observed levitation must be regarded as due to superconductive effects occurring in small domains randomly dispersed throughout the insulating bulk of the investigated cholates. The transition temperatures observed in some of these compounds were higher than those seen in the metals.", "PMID": 981353}
{"id": "pubmed23n0032_12601", "title": "Allosteric linkage and phase transition.", "content": "The thermodynamics of allosteric linkage are observed to be analogous to those of a phase transition. When a phenomenological theory of phase transitions is applied to the allosteric interactions in hemoglobin, a generalized Hill equation is arrived at, including an explicit dependence on temperature. This result is in good agreement with experiments.", "contents": "Allosteric linkage and phase transition. The thermodynamics of allosteric linkage are observed to be analogous to those of a phase transition. When a phenomenological theory of phase transitions is applied to the allosteric interactions in hemoglobin, a generalized Hill equation is arrived at, including an explicit dependence on temperature. This result is in good agreement with experiments.", "PMID": 981354}
{"id": "pubmed23n0032_12602", "title": "Xanthelasma: follow-up on results after surgical excision.", "content": "Xanthelasma palpebrarum is the most common xanthoma and is associated with other xanthomas or hyperlipemia syndromes in only 5 percent of the patients--even though one third of the affected patients have an elevated serum cholesterol level. Surgical excision is simple, safe, leaves minimal scarring, and will be definitive in more than half of the patients being treated for the first time. Reexcision may still be worthwhile if the xanthelasma recurs. However, recurrence is to be anticipated if all 4 eyelids are involved, if there is an underlying hyperlipemia syndrome, or if there has been more than one previous recurrence.", "contents": "Xanthelasma: follow-up on results after surgical excision. Xanthelasma palpebrarum is the most common xanthoma and is associated with other xanthomas or hyperlipemia syndromes in only 5 percent of the patients--even though one third of the affected patients have an elevated serum cholesterol level. Surgical excision is simple, safe, leaves minimal scarring, and will be definitive in more than half of the patients being treated for the first time. Reexcision may still be worthwhile if the xanthelasma recurs. However, recurrence is to be anticipated if all 4 eyelids are involved, if there is an underlying hyperlipemia syndrome, or if there has been more than one previous recurrence.", "PMID": 981398}
{"id": "pubmed23n0032_12603", "title": "Mechanism of upper lid ptosis in the anophthalmic orbit.", "content": "The occurrence of upper lid ptosis in 66 patients in the anophthalmic state was 18 percent, despite an adequate prosthesis. The commonly cited causes for this entity do not hold up under examination. A detailed review of the anatomy of the levator mechanism and of the anatomy of an enucleation suggests that the etiology is really based on the production of a mechanical imbalance in the levator apparatus. Suggestions for management and for the timing of the correction (in relation to other surgical procedures) are made.", "contents": "Mechanism of upper lid ptosis in the anophthalmic orbit. The occurrence of upper lid ptosis in 66 patients in the anophthalmic state was 18 percent, despite an adequate prosthesis. The commonly cited causes for this entity do not hold up under examination. A detailed review of the anatomy of the levator mechanism and of the anatomy of an enucleation suggests that the etiology is really based on the production of a mechanical imbalance in the levator apparatus. Suggestions for management and for the timing of the correction (in relation to other surgical procedures) are made.", "PMID": 981399}
{"id": "pubmed23n0032_12604", "title": "Spherical contracture of fibrous capsules around breast implants. Prevention and treatment.", "content": "A follow-up of 82 patients who had had augumentation mammaplasties is reported. Though the number of patients having had each form of ancillary treatment is small, we believe that intraoperative instillation of triamcinolone and postoperative \"expansion exercises\" are of value in decreasing the incidence of capsular contracture around the implants. When such spherical contracture does occur, the capsule can usually be ruptured simply and quickly by external pressure with the surgeon's hands. This is usually done as an office procedure, without anesthesia. The results are so beneficial that we believe this should be done on many patients. In some it may need to be done repeatedly.", "contents": "Spherical contracture of fibrous capsules around breast implants. Prevention and treatment. A follow-up of 82 patients who had had augumentation mammaplasties is reported. Though the number of patients having had each form of ancillary treatment is small, we believe that intraoperative instillation of triamcinolone and postoperative \"expansion exercises\" are of value in decreasing the incidence of capsular contracture around the implants. When such spherical contracture does occur, the capsule can usually be ruptured simply and quickly by external pressure with the surgeon's hands. This is usually done as an office procedure, without anesthesia. The results are so beneficial that we believe this should be done on many patients. In some it may need to be done repeatedly.", "PMID": 981400}
{"id": "pubmed23n0032_12605", "title": "Secondary correction of the too-high areola and nipple after a mammaplasty.", "content": "Two cases of nipples placed too high by reduction mammaplasties have been corrected by a combination of maneuvers--including (1) shortening the distance from the nipple to the inframammary line, and (2) repositioning of the areola-nipple component or putting in an implant to make the main projection of the breast directly behind the nipple.", "contents": "Secondary correction of the too-high areola and nipple after a mammaplasty. Two cases of nipples placed too high by reduction mammaplasties have been corrected by a combination of maneuvers--including (1) shortening the distance from the nipple to the inframammary line, and (2) repositioning of the areola-nipple component or putting in an implant to make the main projection of the breast directly behind the nipple.", "PMID": 981401}
{"id": "pubmed23n0032_12606", "title": "Compression treatment of hemangiomas.", "content": "Compression therapy appears to be a safe and effective modality for treating involuting and non-involuting hemangiomas. Compression may induce earlier mature resolution in involuting hemangiomas, and may reduce the size of non-involuting hemangiomas--at least temporarily.", "contents": "Compression treatment of hemangiomas. Compression therapy appears to be a safe and effective modality for treating involuting and non-involuting hemangiomas. Compression may induce earlier mature resolution in involuting hemangiomas, and may reduce the size of non-involuting hemangiomas--at least temporarily.", "PMID": 981402}
{"id": "pubmed23n0032_12607", "title": "Soft tissue response to blood-impregnated Proplast.", "content": "The effects of Proplast implants on the soft tissues of rats were studied. The effects from Proplast implants which had been impregnated with blood, plasma, or saline were compared to the effects from implants which had received no pretreatment. No significant differences were found.", "contents": "Soft tissue response to blood-impregnated Proplast. The effects of Proplast implants on the soft tissues of rats were studied. The effects from Proplast implants which had been impregnated with blood, plasma, or saline were compared to the effects from implants which had received no pretreatment. No significant differences were found.", "PMID": 981403}
{"id": "pubmed23n0032_12608", "title": "Triphalangeal thumb.", "content": "In the last 6 years, I have seen 29 patients (37 hands) who had an additional phalanx between the two other phalanges of their thumbs. These triphalangeal thumbs can be divided into opposable and nonopposable thumbs. The epiphyseal plate of the first metacarpal is at the proximal end in the opposable triphalangeal thumb, at the distal end in the nonopposable triphalangeal thumb, at both the distal and proximal ends in the complicated split hand, and at the proximal end in the complicated preaxial polydactyly. Treatment of these cases is described, as well as theories about the origin of the deformity.", "contents": "Triphalangeal thumb. In the last 6 years, I have seen 29 patients (37 hands) who had an additional phalanx between the two other phalanges of their thumbs. These triphalangeal thumbs can be divided into opposable and nonopposable thumbs. The epiphyseal plate of the first metacarpal is at the proximal end in the opposable triphalangeal thumb, at the distal end in the nonopposable triphalangeal thumb, at both the distal and proximal ends in the complicated split hand, and at the proximal end in the complicated preaxial polydactyly. Treatment of these cases is described, as well as theories about the origin of the deformity.", "PMID": 981404}
{"id": "pubmed23n0032_12609", "title": "Pseudoxanthoma elasticum: a dermal elastosis with surgical implications.", "content": "We discuss the condition of pseudoxanthoma elasticum and its differential diagnosis, and we present a patient who had this illness. The redundant skin was surgically removed from her axilla and thigh.", "contents": "Pseudoxanthoma elasticum: a dermal elastosis with surgical implications. We discuss the condition of pseudoxanthoma elasticum and its differential diagnosis, and we present a patient who had this illness. The redundant skin was surgically removed from her axilla and thigh.", "PMID": 981405}
{"id": "pubmed23n0032_12610", "title": "Benign symmetrical lipomatosis. Case report.", "content": "We present a patient who had benign symmetrical lipomatosis. He was treated surgically (6 operations), and the results were satisfactory. we discuss various factors in this disease and suggest the possibility of a nonsurgical treatment being developed in the future.", "contents": "Benign symmetrical lipomatosis. Case report. We present a patient who had benign symmetrical lipomatosis. He was treated surgically (6 operations), and the results were satisfactory. we discuss various factors in this disease and suggest the possibility of a nonsurgical treatment being developed in the future.", "PMID": 981407}
{"id": "pubmed23n0032_12611", "title": "Microsurgery in the prevention of traumatic tattoos.", "content": "We were able to remove most of the foreign body particles from a fresh wound caused by a gunpowder blast to the face, using microsurgical techniques. This prevented a subsequent serious dirt tattoo.", "contents": "Microsurgery in the prevention of traumatic tattoos. We were able to remove most of the foreign body particles from a fresh wound caused by a gunpowder blast to the face, using microsurgical techniques. This prevented a subsequent serious dirt tattoo.", "PMID": 981408}
{"id": "pubmed23n0032_12612", "title": "[Two kinds of dying--existential and psychodynamic aspects of a personal thanatology (author's transl)].", "content": "If we eliminate the area of biological and psychological methods, aspects of human death can be experienced which go far beyond understanding death as a defect, as simple cessation of physiological functions. Only then does the total structure of human death as one of the most important \"border situations\" (Jaspers) of human life become evident. For man, coming to grips with his finality is a task which he cannot escape. This task, which is connected with the ability to experience a future, is an essential characteristic (Scheler) which differentiates man from animals. This is due to the fact that man's spiritual structure has been set in a biological matrix. This double structure of man also implies the possibility of a double cessation of his true nature. In addition to biological death, there is also a spiritual death in human existence where the biological matrix is fully intact: A biologically intact organism, which presents no problem for the internist, is then no longer in a position to participate in a mode of existence with appropriate norms, human dignity and goal orientations. Basically the problem is that the full I-Thou form in terms of Buber and Gebsattel can no longer be realized. Man has isolated himself, maneuvered himself out of this anthropological constitutionality as Zoon Politicon in the sense of Aristotle and put himself on the side-lines. He then no longer lives through and in others and, therefore, no longer participates in the medium and sphere of his only possible existence. The paradox remains, however, that, in contrast to biological death, the man who is existentially dead is condemned to life as many existential philosophers have said. He is condemned to freedom and can use this freedom to manipulate his death, to degrade it by making it an object, to materialize it to a \"'ready-to-hand' thing\" in Heidegger's terms. It can be psychologically energized and used as a defensive weapon; its various forms can be either used against each other or against itself. This was demonstrated by examples taken from the daily practice and from literature.", "contents": "[Two kinds of dying--existential and psychodynamic aspects of a personal thanatology (author's transl)]. If we eliminate the area of biological and psychological methods, aspects of human death can be experienced which go far beyond understanding death as a defect, as simple cessation of physiological functions. Only then does the total structure of human death as one of the most important \"border situations\" (Jaspers) of human life become evident. For man, coming to grips with his finality is a task which he cannot escape. This task, which is connected with the ability to experience a future, is an essential characteristic (Scheler) which differentiates man from animals. This is due to the fact that man's spiritual structure has been set in a biological matrix. This double structure of man also implies the possibility of a double cessation of his true nature. In addition to biological death, there is also a spiritual death in human existence where the biological matrix is fully intact: A biologically intact organism, which presents no problem for the internist, is then no longer in a position to participate in a mode of existence with appropriate norms, human dignity and goal orientations. Basically the problem is that the full I-Thou form in terms of Buber and Gebsattel can no longer be realized. Man has isolated himself, maneuvered himself out of this anthropological constitutionality as Zoon Politicon in the sense of Aristotle and put himself on the side-lines. He then no longer lives through and in others and, therefore, no longer participates in the medium and sphere of his only possible existence. The paradox remains, however, that, in contrast to biological death, the man who is existentially dead is condemned to life as many existential philosophers have said. He is condemned to freedom and can use this freedom to manipulate his death, to degrade it by making it an object, to materialize it to a \"'ready-to-hand' thing\" in Heidegger's terms. It can be psychologically energized and used as a defensive weapon; its various forms can be either used against each other or against itself. This was demonstrated by examples taken from the daily practice and from literature.", "PMID": 981494}
{"id": "pubmed23n0032_12613", "title": "[Reflections on and initial experiences with the teaching of psychotherapy after the new regulation for medical licensing (author's transl)].", "content": "This study discusses the first psychotherapeutic-oriented portion of the practical course for psychosomatics/psychotherapy which was held in seminar form in small groups. The material for the course was prepared cuts from vitiotapes of first and second therapeutic sessions conducted in a psychotherapeutic outpatient clinic. To a large extent, the initial experience tends to speak for our teaching plan which basically refrained from initially mediating theory. On the other hand, it was also necessary to cutback the original plan in an attempt to use the \"countertransference reactions\" of the students to better understand the patients. Special attention was given to the affective reactions of the students in the individual groups since a certain dependency on the specific teaching style of the seminar leader was to be expected. Occasionally, there was danger of overestimating the tolerance of the students to their tensions and fears. This intensified the defense reactions. The experience up until now caused us to modify our plan in a few points.", "contents": "[Reflections on and initial experiences with the teaching of psychotherapy after the new regulation for medical licensing (author's transl)]. This study discusses the first psychotherapeutic-oriented portion of the practical course for psychosomatics/psychotherapy which was held in seminar form in small groups. The material for the course was prepared cuts from vitiotapes of first and second therapeutic sessions conducted in a psychotherapeutic outpatient clinic. To a large extent, the initial experience tends to speak for our teaching plan which basically refrained from initially mediating theory. On the other hand, it was also necessary to cutback the original plan in an attempt to use the \"countertransference reactions\" of the students to better understand the patients. Special attention was given to the affective reactions of the students in the individual groups since a certain dependency on the specific teaching style of the seminar leader was to be expected. Occasionally, there was danger of overestimating the tolerance of the students to their tensions and fears. This intensified the defense reactions. The experience up until now caused us to modify our plan in a few points.", "PMID": 981495}
{"id": "pubmed23n0032_12614", "title": "[\"Therapeutic play\" als clinical short-term group therapy in rehabilitative and preventive internal medicine (author's transl)].", "content": "A method of therapeutic play was presented which was used with young patients in treatment at a health resort. The patients were diagnosed as suffering from the vegetative syndrome. They also exhibited psychological problems such as difficulty in establishing interpersonal relationships, inhibition and depressive tendencies. This method consisting of a three-stage program of self-perception, interaction and role playing has been used successfully as short-term group therapy in the context of treatment at a health resort as the investigation of 49 participants shows.", "contents": "[\"Therapeutic play\" als clinical short-term group therapy in rehabilitative and preventive internal medicine (author's transl)]. A method of therapeutic play was presented which was used with young patients in treatment at a health resort. The patients were diagnosed as suffering from the vegetative syndrome. They also exhibited psychological problems such as difficulty in establishing interpersonal relationships, inhibition and depressive tendencies. This method consisting of a three-stage program of self-perception, interaction and role playing has been used successfully as short-term group therapy in the context of treatment at a health resort as the investigation of 49 participants shows.", "PMID": 981496}
{"id": "pubmed23n0032_12615", "title": "Presidential Address-1976. Social support as a moderator of life stress.", "content": "Social support is defined as information leading the subject to believe that he is cared for and loved, esteemed, and a member of a network of mutual obligations. The evidence that supportive interactions among people are protective against the health consequences of life stress is reviewed. It appears that social support can protect people in crisis from a wide variety of pathological states: from low birth weight to death, from arthritis through tuberculosis to depression, alcoholism, and the social breakdown syndrome. Furthermore, social support may reduce the amount of medication required, accelerate recovery, and facilitate compliance with prescribed medical regimens.", "contents": "Presidential Address-1976. Social support as a moderator of life stress. Social support is defined as information leading the subject to believe that he is cared for and loved, esteemed, and a member of a network of mutual obligations. The evidence that supportive interactions among people are protective against the health consequences of life stress is reviewed. It appears that social support can protect people in crisis from a wide variety of pathological states: from low birth weight to death, from arthritis through tuberculosis to depression, alcoholism, and the social breakdown syndrome. Furthermore, social support may reduce the amount of medication required, accelerate recovery, and facilitate compliance with prescribed medical regimens.", "PMID": 981490}
{"id": "pubmed23n0032_12616", "title": "[Peculiarities of a consultative service and a psychosomatic consultation-hour at a dermatological university clinic (author's transl)].", "content": "When organizing a consultative service and a psychosomatic consultation-hour at a dermatological clinic the following (specific?) peculiarities resulted: 1) the fellow doctors at the clinic at first only sent somatically untreatable cases and 2) were afraid of a correction of their diagnosis. 3) By way of identification they were faced with their own proper problems. The patients understood themselves to be physically ill and at first could not be approached regarding to their subconscious conflicts. The actual conduct of the patients in the wards and their attitude to the physicians was used in a psychodiagnostical way (whereas analytical interpretations were dispensed with) and was used therapeutically to elucidate the psycho-genesis of their affliction. Only with some singular patients suffering from skin-diseases a proper analysis could be performed for research purposes; most of them had to be treated simultaneously dermatologically as well as psychoanalytically (in a modified way). Merely with patients suffering from andrological troubles group-therapy along analytical lines was feasible and proved to be successful.", "contents": "[Peculiarities of a consultative service and a psychosomatic consultation-hour at a dermatological university clinic (author's transl)]. When organizing a consultative service and a psychosomatic consultation-hour at a dermatological clinic the following (specific?) peculiarities resulted: 1) the fellow doctors at the clinic at first only sent somatically untreatable cases and 2) were afraid of a correction of their diagnosis. 3) By way of identification they were faced with their own proper problems. The patients understood themselves to be physically ill and at first could not be approached regarding to their subconscious conflicts. The actual conduct of the patients in the wards and their attitude to the physicians was used in a psychodiagnostical way (whereas analytical interpretations were dispensed with) and was used therapeutically to elucidate the psycho-genesis of their affliction. Only with some singular patients suffering from skin-diseases a proper analysis could be performed for research purposes; most of them had to be treated simultaneously dermatologically as well as psychoanalytically (in a modified way). Merely with patients suffering from andrological troubles group-therapy along analytical lines was feasible and proved to be successful.", "PMID": 981497}
{"id": "pubmed23n0032_12617", "title": "Body image disturbances in anorexia nervosa and obesity.", "content": "Measures were made of body image and personality features in patients with anorexia nervosa and obesity. It was hypothesized that obese and anorexic patients would display similar body image disturbances characterized by relative overestimation of body size in comparison with control subjects. Body image was measured by both a distorting photograph technique (a general measure) and a visual size estimation apparatus (for specific body regions). Personality features were assessed by the Eysenck Personality Inventory and a modified version of Rotter's Locus of Control Scale. Results indicated that both obese (N=16) and anorexic (N=18) subjects significantly differed from three control groups (P less than 0.01) in body size estimation on a general measure of body image. A measure of specific body regions did not differentiate between groups. For anorexic and obese patients, body size estimates were significantly correlated with personality features.", "contents": "Body image disturbances in anorexia nervosa and obesity. Measures were made of body image and personality features in patients with anorexia nervosa and obesity. It was hypothesized that obese and anorexic patients would display similar body image disturbances characterized by relative overestimation of body size in comparison with control subjects. Body image was measured by both a distorting photograph technique (a general measure) and a visual size estimation apparatus (for specific body regions). Personality features were assessed by the Eysenck Personality Inventory and a modified version of Rotter's Locus of Control Scale. Results indicated that both obese (N=16) and anorexic (N=18) subjects significantly differed from three control groups (P less than 0.01) in body size estimation on a general measure of body image. A measure of specific body regions did not differentiate between groups. For anorexic and obese patients, body size estimates were significantly correlated with personality features.", "PMID": 981491}
{"id": "pubmed23n0032_12618", "title": "[On the intellectual capacity of patients suffering from ulcerative colitis--a comparative study using the HAWIE--(author's transl)].", "content": "Differing reports in the literature on the intellectual capabilities of patients with unspecific ulcerative colitis caused us to undertake a controlled test-psychological study. 30 patients with ulcerative colitis and 30 \"healthy\" control persons were tested with the Hamburg-Wechsler-Intelligenztest f\u00fcr Erwachsene (HAWIE). The patients and the controls were matched as regards age, sex, family status, education, professional status and monthly income. A statistical comparison using the Wilcoxon matched-pairs signed-ranks test showed that the two groups did not differ significantly in their intelligence quotients. Our results seem to indicate that the assumption of extraordinary intellectual capabilities of patients with ulcerative colitis is not tenable.", "contents": "[On the intellectual capacity of patients suffering from ulcerative colitis--a comparative study using the HAWIE--(author's transl)]. Differing reports in the literature on the intellectual capabilities of patients with unspecific ulcerative colitis caused us to undertake a controlled test-psychological study. 30 patients with ulcerative colitis and 30 \"healthy\" control persons were tested with the Hamburg-Wechsler-Intelligenztest f\u00fcr Erwachsene (HAWIE). The patients and the controls were matched as regards age, sex, family status, education, professional status and monthly income. A statistical comparison using the Wilcoxon matched-pairs signed-ranks test showed that the two groups did not differ significantly in their intelligence quotients. Our results seem to indicate that the assumption of extraordinary intellectual capabilities of patients with ulcerative colitis is not tenable.", "PMID": 981498}
{"id": "pubmed23n0032_12619", "title": "Death from fright: report of a case and literature review.", "content": "A case report is presented illustrating the thesis that fear of fantasy material may trigger off a physiological process leading to death.", "contents": "Death from fright: report of a case and literature review. A case report is presented illustrating the thesis that fear of fantasy material may trigger off a physiological process leading to death.", "PMID": 981493}
{"id": "pubmed23n0032_12620", "title": "Facial expression and imagery in depression: an electromyographic study.", "content": "When subjects are instructed to self-generate happy, sad, and angry imagery, discrete patterns of facial muscle activity can be detected using electromyographic (EMG) procedures. Prior research from this laboratory suggests that depressed subjects show attenuated facial EMG patterns during imagery conditions, particularly during happy imagery. In the present experiment, 12 depressed subjects and 12 matched normals were requested to generate happy and sad imagery, first with the instruction to simply \"think\" about the imagery, and then to self-regulate the affective state by \"reexperiencing the feelings\" associated with the imagery. Continuous recordings of facial EMG were obtained from the corrugator, zygomatic major, depressor anguli oris, and mentalis muscle regions. It was hypothesized that (a) these muscle sites would reliably differentiate between happy and sad imagery. (b) the instruction to self-generate the affective feeling state would produce greater EMG differences than the \"think\" instructions, and (c) the \"think\" instructions would be a more sensitive indicator of the difference between depressed and nondepressed subjects, especially for happy imagery. All three hypotheses were confirmed. The application of facial electromyography to the assessment of normal and clinical mood states, and the role of facial muscle patterning in the subjective experience of emotion, are discussed.", "contents": "Facial expression and imagery in depression: an electromyographic study. When subjects are instructed to self-generate happy, sad, and angry imagery, discrete patterns of facial muscle activity can be detected using electromyographic (EMG) procedures. Prior research from this laboratory suggests that depressed subjects show attenuated facial EMG patterns during imagery conditions, particularly during happy imagery. In the present experiment, 12 depressed subjects and 12 matched normals were requested to generate happy and sad imagery, first with the instruction to simply \"think\" about the imagery, and then to self-regulate the affective state by \"reexperiencing the feelings\" associated with the imagery. Continuous recordings of facial EMG were obtained from the corrugator, zygomatic major, depressor anguli oris, and mentalis muscle regions. It was hypothesized that (a) these muscle sites would reliably differentiate between happy and sad imagery. (b) the instruction to self-generate the affective feeling state would produce greater EMG differences than the \"think\" instructions, and (c) the \"think\" instructions would be a more sensitive indicator of the difference between depressed and nondepressed subjects, especially for happy imagery. All three hypotheses were confirmed. The application of facial electromyography to the assessment of normal and clinical mood states, and the role of facial muscle patterning in the subjective experience of emotion, are discussed.", "PMID": 981492}
{"id": "pubmed23n0032_12621", "title": "Cost and benefits of lizard thermoregulation.", "content": "Lizards thermoregulate by behavioral and physiological adjustments. The resultant control over metabolic processes is generally assumed to be beneficial. However, these thermoregulatory adjustments have associated costs which, if extensive, make thermoregulation impractical. We extend this idea into an abstract mathematical, cost-benefit model of thermoregulation in lizards. Investigation of the model leads to a set of predictions which includes: (1) the physiologically optimal temperature is not always the ecologically optimal temperature; (2) thermoregulation is beneficial only when associated costs are low; (3) thermal specialists will normally thermoregulate more carefully than thermal generalists unless costs are high; and (4) lizards will thermoregulate more carefully if productivity of the habitat is increased or if exploitation competition is reduced. Data on lizards, where available, generally agree with these predicitions.", "contents": "Cost and benefits of lizard thermoregulation. Lizards thermoregulate by behavioral and physiological adjustments. The resultant control over metabolic processes is generally assumed to be beneficial. However, these thermoregulatory adjustments have associated costs which, if extensive, make thermoregulation impractical. We extend this idea into an abstract mathematical, cost-benefit model of thermoregulation in lizards. Investigation of the model leads to a set of predictions which includes: (1) the physiologically optimal temperature is not always the ecologically optimal temperature; (2) thermoregulation is beneficial only when associated costs are low; (3) thermal specialists will normally thermoregulate more carefully than thermal generalists unless costs are high; and (4) lizards will thermoregulate more carefully if productivity of the habitat is increased or if exploitation competition is reduced. Data on lizards, where available, generally agree with these predicitions.", "PMID": 981504}
{"id": "pubmed23n0032_12622", "title": "[Criminologic manifesto].", "content": "There is conventional crime and unconventional crime. The first is mostly made up of offences against persons, property, morals and public order; the second of crimes committed under cover of official positions; against international laws; by \"intelligence services\"; terrorism; economic and financial frauds; illicit trade; criminal corruption at high levels; discriminatory practices; pollution; illicit traffic drugs, etc.. The vast majority of them are defined as criminal offences by national penal codes and special laws, but largely go unpunished. Unconventional crime cannot be explained by the traditional references to the personality of the offender. The explanation for the failure of contemporary criminology lies mostly in regarding the phenomenon of criminality as the ensemble of crimes which may be individually explained, instead of considering it as a sociopolitical phenomenon. If criminology is to survive, it must realize that the conventional is only one aspect of the sociopolitical phenomenon of crime, and that the study of unconventional crime demands an approach that the self-satisfied criminologies of our time cannot provide. The discussion and the study on unconventional crime will raise political difficulties, but the time has come that criminology and criminal justice face the fact that science can no longer be (or assume to be) neutral.", "contents": "[Criminologic manifesto]. There is conventional crime and unconventional crime. The first is mostly made up of offences against persons, property, morals and public order; the second of crimes committed under cover of official positions; against international laws; by \"intelligence services\"; terrorism; economic and financial frauds; illicit trade; criminal corruption at high levels; discriminatory practices; pollution; illicit traffic drugs, etc.. The vast majority of them are defined as criminal offences by national penal codes and special laws, but largely go unpunished. Unconventional crime cannot be explained by the traditional references to the personality of the offender. The explanation for the failure of contemporary criminology lies mostly in regarding the phenomenon of criminality as the ensemble of crimes which may be individually explained, instead of considering it as a sociopolitical phenomenon. If criminology is to survive, it must realize that the conventional is only one aspect of the sociopolitical phenomenon of crime, and that the study of unconventional crime demands an approach that the self-satisfied criminologies of our time cannot provide. The discussion and the study on unconventional crime will raise political difficulties, but the time has come that criminology and criminal justice face the fact that science can no longer be (or assume to be) neutral.", "PMID": 981506}
{"id": "pubmed23n0032_12623", "title": "A generalization of the clonal survival models: equations for the families of curves obtained with fractionated irradiation.", "content": "The survival curves obtained when cellular recovery follows various first radiation dose deliveries DI seem, when semi-logarithmically plotted, to be translated from the part of the curve corresponding to an unfractionated irradiation beyond a dose DR. A possible assumption consistent with such experimental observations is proposed which allows the generalization of any survival model S = f (D). The derived equation S = f (DR + D - DI) f (DI)/f (DR) is convenient for the whole family of experimental survival curves involving cellular damage repairs when the first radiation doses vary. All the parameters of the family equation can be simultaneously fitted so that their reliability is increased. The generalized equations are given for the four following models: two-hits targets, Chadwick and Leenhouts, Green and Burki, Wider\u00f6e. As an example, the Chadwick and Leenhouts generalized model parameters are fitted to a family of experimental survival curves concerning Chlorella cells exposed to fractionated and continuous gamma irradiation. The fittings are presented with their confidence limits and are briefly discussed.", "contents": "A generalization of the clonal survival models: equations for the families of curves obtained with fractionated irradiation. The survival curves obtained when cellular recovery follows various first radiation dose deliveries DI seem, when semi-logarithmically plotted, to be translated from the part of the curve corresponding to an unfractionated irradiation beyond a dose DR. A possible assumption consistent with such experimental observations is proposed which allows the generalization of any survival model S = f (D). The derived equation S = f (DR + D - DI) f (DI)/f (DR) is convenient for the whole family of experimental survival curves involving cellular damage repairs when the first radiation doses vary. All the parameters of the family equation can be simultaneously fitted so that their reliability is increased. The generalized equations are given for the four following models: two-hits targets, Chadwick and Leenhouts, Green and Burki, Wider\u00f6e. As an example, the Chadwick and Leenhouts generalized model parameters are fitted to a family of experimental survival curves concerning Chlorella cells exposed to fractionated and continuous gamma irradiation. The fittings are presented with their confidence limits and are briefly discussed.", "PMID": 981511}
{"id": "pubmed23n0032_12624", "title": "X-ray-induced recombination during oogenesis in the silkworm (Bombyx mori l.).", "content": "The physical induction of recombinational events has been studied in the female silkworm (Bombyx mori), in which crossing-over does not normally occur. Female silkworms heterozygous in the trans type of two egg-color genes, pe (V-0.0) and re (V-31.7), received a single dose of X-rays (1000 R) at various developmental stages. Then they were crossed to marked males homozygous for both genes. The results indicated that X-rays increase the occurrence of recombinational events in silkworm females from first instar larvae to late stage pupae. The spontaneous frequency of exchange type recombinants in the control series was 2.5 X 10(-5), while after irradiation the frequency of these recombinants was up to 38.8 X 10(-5). The sensitive stage to X-ray-induced recombinational events was late stage larvae from fourth to fifth instar. Exchange (cross-over) type recombinants were about three times more frequent than interchange types among the 122 recombinants recovered. The biological significance of the present finding is discussed.", "contents": "X-ray-induced recombination during oogenesis in the silkworm (Bombyx mori l.). The physical induction of recombinational events has been studied in the female silkworm (Bombyx mori), in which crossing-over does not normally occur. Female silkworms heterozygous in the trans type of two egg-color genes, pe (V-0.0) and re (V-31.7), received a single dose of X-rays (1000 R) at various developmental stages. Then they were crossed to marked males homozygous for both genes. The results indicated that X-rays increase the occurrence of recombinational events in silkworm females from first instar larvae to late stage pupae. The spontaneous frequency of exchange type recombinants in the control series was 2.5 X 10(-5), while after irradiation the frequency of these recombinants was up to 38.8 X 10(-5). The sensitive stage to X-ray-induced recombinational events was late stage larvae from fourth to fifth instar. Exchange (cross-over) type recombinants were about three times more frequent than interchange types among the 122 recombinants recovered. The biological significance of the present finding is discussed.", "PMID": 981512}
{"id": "pubmed23n0032_12625", "title": "Calculation of specific energies of incorporated 239Pu and 131I in accordance with the concept of the critical cell.", "content": "A better understanding of the effects of energy deposited in cells by incorporated isotopes can be expected from an analysis of differential cell doses. With this thought in mind, the distributions of specific energies in tissue were calculated for 239Pu and 131I. A program written in Fortran IV makes use of a matrix of spherical cells and cell nuclei of 10 and 8 mum diameter, respectively. The cells are arranged in close-packed structure. The radioactivity is considered as being compiled to point sources of 1 dps activity. The sources are located in the common centers of cells and nuclei. The calculations yield discrete values of specific energy using the mass of the nuclei for mass of reference. The numbers of cell nuclei receiving given amounts of specific energy are functions of the specific activity of the isotopes in the tissue. The specific activity is varied by changing the number of sources per g of tissue. The program also allows to calculate the numbers of cell nuclei with zero energy deposition. From the 1 dps point sources an average specific energy of 831 rads/h results for plutonium for cell nuclei within the range of the 5.14 MeV alpha-particles. For iodine, the value is 35 mrads/h within the range of the beta-particles of 188 KeV mean energy. If the volumes irradiated by the sources begin to overlap these values begin to increase accordingly.", "contents": "Calculation of specific energies of incorporated 239Pu and 131I in accordance with the concept of the critical cell. A better understanding of the effects of energy deposited in cells by incorporated isotopes can be expected from an analysis of differential cell doses. With this thought in mind, the distributions of specific energies in tissue were calculated for 239Pu and 131I. A program written in Fortran IV makes use of a matrix of spherical cells and cell nuclei of 10 and 8 mum diameter, respectively. The cells are arranged in close-packed structure. The radioactivity is considered as being compiled to point sources of 1 dps activity. The sources are located in the common centers of cells and nuclei. The calculations yield discrete values of specific energy using the mass of the nuclei for mass of reference. The numbers of cell nuclei receiving given amounts of specific energy are functions of the specific activity of the isotopes in the tissue. The specific activity is varied by changing the number of sources per g of tissue. The program also allows to calculate the numbers of cell nuclei with zero energy deposition. From the 1 dps point sources an average specific energy of 831 rads/h results for plutonium for cell nuclei within the range of the 5.14 MeV alpha-particles. For iodine, the value is 35 mrads/h within the range of the beta-particles of 188 KeV mean energy. If the volumes irradiated by the sources begin to overlap these values begin to increase accordingly.", "PMID": 981513}
{"id": "pubmed23n0032_12626", "title": "[Prospects of therapeutic intervention in drug addiction in prisons].", "content": "The non-penalization envisaged by the new law concerning the use and possession of drugs, will not cause the population of drug addicts to disappear from the prisons; it is however to be expected a qualitative modification of them, with the drug addicts clinically in most serious and persistent conditions and more involved with the criminal subculture, gradually increasing in number. Such individuals will represent a group of potential customers much different from that found up to now, and their more meaningful features will concern their age (higher), polytoxicomania (almost always resulting in heroinism), the abandonment of all family ties, of the pristine social and cultural status, of work, to acquire a new role, where identity is extremely ambiguous and uncertain. As concerns the treatment hypotheses, the less involved users being no longer dealth with--upon whom the attention of operators had been chiefly centered, and on whom the prospects of a psychotherapeutic success had been founded--only two solutions are regarded as implementable in the future: either to give up all intramural treatments, with the exception of those having a medical character, and, extramurally, to provide for the supply (controlled and through dispensaries) of heroin or substitute drugs, so as to reduce as much as possible the danger drug addicts represent for society; or to establish therapeutic or intramural communities, forming the one instrument capable of suggesting or conditioning new identity patterns, as an initial stage of an analogous institutional treatment to be continued when the convict leaves the prison.", "contents": "[Prospects of therapeutic intervention in drug addiction in prisons]. The non-penalization envisaged by the new law concerning the use and possession of drugs, will not cause the population of drug addicts to disappear from the prisons; it is however to be expected a qualitative modification of them, with the drug addicts clinically in most serious and persistent conditions and more involved with the criminal subculture, gradually increasing in number. Such individuals will represent a group of potential customers much different from that found up to now, and their more meaningful features will concern their age (higher), polytoxicomania (almost always resulting in heroinism), the abandonment of all family ties, of the pristine social and cultural status, of work, to acquire a new role, where identity is extremely ambiguous and uncertain. As concerns the treatment hypotheses, the less involved users being no longer dealth with--upon whom the attention of operators had been chiefly centered, and on whom the prospects of a psychotherapeutic success had been founded--only two solutions are regarded as implementable in the future: either to give up all intramural treatments, with the exception of those having a medical character, and, extramurally, to provide for the supply (controlled and through dispensaries) of heroin or substitute drugs, so as to reduce as much as possible the danger drug addicts represent for society; or to establish therapeutic or intramural communities, forming the one instrument capable of suggesting or conditioning new identity patterns, as an initial stage of an analogous institutional treatment to be continued when the convict leaves the prison.", "PMID": 981509}
{"id": "pubmed23n0032_12627", "title": "Microdosimetric structure of heavy ion tracks in tissue.", "content": "Energy dissipation in tracks of high energy heavy ions in tissue shows a lateral spread of several to many microns depending on the energy of the primary particle. Complete dosimetric characterization, therefore, requires in addition to the Linear Energy Transfer (LET) information on the radial energy distribution. The theory of track structure distinguishes two regions: core and penumbra. The core is a narrow central zone with a radius in tissue far below 1 micron where energy deposition occurs mainly in processes of excitation and electron plasma oscillation. According to the Equipartition Principle, half of the total energy dissipation accrues in this manner. The penumbra is a peripheral zone enveloping the core where energy deposition occurs mainly in ionization events by energetic secondary electrons released by the primary particle in the center of the core traveling at rather high speed thus spreading laterally. The extension of the penumbra depends in a complex manner on the maximum transferable energy to electrons which in turn depends on the speed of the primary particle. Local energy density in the penumbra decreases with the square of increasing radius. It therefore amounts only to a very small fraction of the core density already a few microns away from the center. In general terms, track structure can be described as exhibiting a core of enormous energy density with lateral dimensions remaining entirely on the submicroscopic level surrounded by a penumbra where energy density drops precipitously to very small levels. The relationships are illustrated with micrographs of different sections of a heavy particle track in nuclear emulsion and their counterpart graphical plots.", "contents": "Microdosimetric structure of heavy ion tracks in tissue. Energy dissipation in tracks of high energy heavy ions in tissue shows a lateral spread of several to many microns depending on the energy of the primary particle. Complete dosimetric characterization, therefore, requires in addition to the Linear Energy Transfer (LET) information on the radial energy distribution. The theory of track structure distinguishes two regions: core and penumbra. The core is a narrow central zone with a radius in tissue far below 1 micron where energy deposition occurs mainly in processes of excitation and electron plasma oscillation. According to the Equipartition Principle, half of the total energy dissipation accrues in this manner. The penumbra is a peripheral zone enveloping the core where energy deposition occurs mainly in ionization events by energetic secondary electrons released by the primary particle in the center of the core traveling at rather high speed thus spreading laterally. The extension of the penumbra depends in a complex manner on the maximum transferable energy to electrons which in turn depends on the speed of the primary particle. Local energy density in the penumbra decreases with the square of increasing radius. It therefore amounts only to a very small fraction of the core density already a few microns away from the center. In general terms, track structure can be described as exhibiting a core of enormous energy density with lateral dimensions remaining entirely on the submicroscopic level surrounded by a penumbra where energy density drops precipitously to very small levels. The relationships are illustrated with micrographs of different sections of a heavy particle track in nuclear emulsion and their counterpart graphical plots.", "PMID": 981514}
{"id": "pubmed23n0032_12628", "title": "90Sr + 90Y bremsstrahlung efficiency predicted for humans.", "content": "Bremsstrahlung x-rays are produced when energetic beta-particles are emitted and absorbed. Measurements of total-body bremsstrahlung efficiencey (x-ray photon output per muCi 90Sr in the body, relative to that in water) have been made in the intact mouse, rat, rabbit, and dog sacrificed 2 weeks after the injection of 90Sr + 85Sr. Efficiencies were determined by a comparison of the bremsstrahlung output from 90Sr + its daughter 90Y and the gamma-ray emission of 85Sr. Results were checked by a beta-assay of the ashed animals. Bremsstrahlung efficiencies averaged 1.10 in a 0.04 kg mouse, 1.14 in a 0.13 kg rat, 1.23 in a 2.6 kg rabbit, and 1.32 in an 8.5 kg beagle. Extrapolating to a 70 kg human, a relative bremsstrahlung efficiency of about 1.4 is predicted. An estimate was made of the 90Sr body content in a former dial painter based on in vivo counting and a bremsstrahlung efficiency of 1.39 predicted for a 55 kg human female by these animal data. Our value of 1.42 +/- 0.08 muCi 90Sr was in good agreement with corresponding results reported for this subject by 8 other laboratories.", "contents": "90Sr + 90Y bremsstrahlung efficiency predicted for humans. Bremsstrahlung x-rays are produced when energetic beta-particles are emitted and absorbed. Measurements of total-body bremsstrahlung efficiencey (x-ray photon output per muCi 90Sr in the body, relative to that in water) have been made in the intact mouse, rat, rabbit, and dog sacrificed 2 weeks after the injection of 90Sr + 85Sr. Efficiencies were determined by a comparison of the bremsstrahlung output from 90Sr + its daughter 90Y and the gamma-ray emission of 85Sr. Results were checked by a beta-assay of the ashed animals. Bremsstrahlung efficiencies averaged 1.10 in a 0.04 kg mouse, 1.14 in a 0.13 kg rat, 1.23 in a 2.6 kg rabbit, and 1.32 in an 8.5 kg beagle. Extrapolating to a 70 kg human, a relative bremsstrahlung efficiency of about 1.4 is predicted. An estimate was made of the 90Sr body content in a former dial painter based on in vivo counting and a bremsstrahlung efficiency of 1.39 predicted for a 55 kg human female by these animal data. Our value of 1.42 +/- 0.08 muCi 90Sr was in good agreement with corresponding results reported for this subject by 8 other laboratories.", "PMID": 981515}
{"id": "pubmed23n0032_12629", "title": "Determination of the uranium content of environmental samples by gamma spectroscopy of the 1.0015 MeV radiation in 234U.", "content": "A method of determining the uranium content of environmental samples by gamma spectroscopy of the 1.0015 MeV gamma radiation of the 234Pam (beta-) 234U leads to 234U daughter decay is described. The detection limit is 1 nCi/kg sample. The transition probability has been determined to be 0.87 +/- 0.03%.", "contents": "Determination of the uranium content of environmental samples by gamma spectroscopy of the 1.0015 MeV radiation in 234U. A method of determining the uranium content of environmental samples by gamma spectroscopy of the 1.0015 MeV gamma radiation of the 234Pam (beta-) 234U leads to 234U daughter decay is described. The detection limit is 1 nCi/kg sample. The transition probability has been determined to be 0.87 +/- 0.03%.", "PMID": 981516}
{"id": "pubmed23n0032_12630", "title": "Population dose from natural radionuclides in phosphate fertilizers.", "content": "The natural radionuclide content of mineral phosphate fertilizers has been determined gammaspectrometrically. The investigations comprised ca. 70% of the mineral phosphate fertilizers authorized and used in the Federal Republic of Germany (FRG). At maximum, we found specific activities of 62 nCi Unat/kg, 23 nCi 226Ra/kg, 1.6 nCi Thnat/kg and 262 nCi 40K/kg. The mean values, weighted by the percentual agricultural consumption of the main phosphate fertilizer groups in 1973/74 and related to the phosphate content, amounted to 58, 40, 2, and 584 nCi/kg P2O5 for Unat, 226Ra, Thnat, and 40K respectively. This resulted in an annual distribution due to phosphate fertilizing of about 3.9 muCi Unat, 2.7 muCi 226Ra, 0.1 muCi Thnat, and 39.9 muCi 40K per ha of arable or pasture land in 1973/74 on the average. From these values the air dose rates over agricultural areas have been estimated under extreme conservative assumptions resulting in an additional external exposure of members of the population of 0.02 mrd/a on the average and 0.4 mrd/a in the region of highest phosphate fertilizing intensity. If it is assumed that radium contained in phosphate fertilizers were completely accumulated in the soils during the last 80 years, this value would be raised to 0.3 mrd/a on the average. The occupational external radiation exposure due to natural radionuclides contained in phosphate fertilizers was estimated to be 0.1 mrd/a on the average and 2.3 mrd/a at maximum for persons working in agriculture. These estimates show that natural radionuclides in phosphate fertilizers contribute but very little to the mean terrestrial radiation exposure of the population which is 50 to 55 mrd/a in Germany. Only for the small group of persons working in fertilizer production plants or storehouses a significant increase of the external radiation exposure has to be expected which could reach a doubling of the mean natural exposure value.", "contents": "Population dose from natural radionuclides in phosphate fertilizers. The natural radionuclide content of mineral phosphate fertilizers has been determined gammaspectrometrically. The investigations comprised ca. 70% of the mineral phosphate fertilizers authorized and used in the Federal Republic of Germany (FRG). At maximum, we found specific activities of 62 nCi Unat/kg, 23 nCi 226Ra/kg, 1.6 nCi Thnat/kg and 262 nCi 40K/kg. The mean values, weighted by the percentual agricultural consumption of the main phosphate fertilizer groups in 1973/74 and related to the phosphate content, amounted to 58, 40, 2, and 584 nCi/kg P2O5 for Unat, 226Ra, Thnat, and 40K respectively. This resulted in an annual distribution due to phosphate fertilizing of about 3.9 muCi Unat, 2.7 muCi 226Ra, 0.1 muCi Thnat, and 39.9 muCi 40K per ha of arable or pasture land in 1973/74 on the average. From these values the air dose rates over agricultural areas have been estimated under extreme conservative assumptions resulting in an additional external exposure of members of the population of 0.02 mrd/a on the average and 0.4 mrd/a in the region of highest phosphate fertilizing intensity. If it is assumed that radium contained in phosphate fertilizers were completely accumulated in the soils during the last 80 years, this value would be raised to 0.3 mrd/a on the average. The occupational external radiation exposure due to natural radionuclides contained in phosphate fertilizers was estimated to be 0.1 mrd/a on the average and 2.3 mrd/a at maximum for persons working in agriculture. These estimates show that natural radionuclides in phosphate fertilizers contribute but very little to the mean terrestrial radiation exposure of the population which is 50 to 55 mrd/a in Germany. Only for the small group of persons working in fertilizer production plants or storehouses a significant increase of the external radiation exposure has to be expected which could reach a doubling of the mean natural exposure value.", "PMID": 981517}
{"id": "pubmed23n0032_12631", "title": "Determination of heavy elements in organic substrates by x-ray absorption.", "content": "Selective X-ray absorption method has been worked out to determine concentration of heavy elements in organic substances. The method is applicable in vivo.", "contents": "Determination of heavy elements in organic substrates by x-ray absorption. Selective X-ray absorption method has been worked out to determine concentration of heavy elements in organic substances. The method is applicable in vivo.", "PMID": 981518}
{"id": "pubmed23n0032_12632", "title": "Protein metabolism in calotes versicolor (Daud) exposed to various stresses.", "content": "Protein and amino acid contents of liver of normal (untreated) adult male garden lizard, Calotes versicolor, were quantitatively determined by the method of Lowry et al. and ninhydrin reaction, respectively, and compared with the protein and amino acid contents in the liver of adult male garden lizards exposed to cold, heat, UV- and gamma-radiations. With a decrease in protein contents, a simultaneous increase in the amino acid contents was observed. The decrease in protein content was 80, 60, 30 and 20% after exposure to cold, heat, UV- and gamma-radiations, respectively. Consequently, the increase in amino acid content was 57, 40, 20 and 5% after exposure to cold, heat, UV- and gamma-radiations, respectively.", "contents": "Protein metabolism in calotes versicolor (Daud) exposed to various stresses. Protein and amino acid contents of liver of normal (untreated) adult male garden lizard, Calotes versicolor, were quantitatively determined by the method of Lowry et al. and ninhydrin reaction, respectively, and compared with the protein and amino acid contents in the liver of adult male garden lizards exposed to cold, heat, UV- and gamma-radiations. With a decrease in protein contents, a simultaneous increase in the amino acid contents was observed. The decrease in protein content was 80, 60, 30 and 20% after exposure to cold, heat, UV- and gamma-radiations, respectively. Consequently, the increase in amino acid content was 57, 40, 20 and 5% after exposure to cold, heat, UV- and gamma-radiations, respectively.", "PMID": 981519}
{"id": "pubmed23n0032_12633", "title": "Multiple chronic benign pulmonary nodules.", "content": "Four cases are discussed in which were found unusual multiple chronic pulmonary nodules: leiomyomatous hamartomas, rheumatoid nodules, multiple histoplasmomas, and possible multiple plasma cell granulomas (hyalinizing pulmonary nodules). In each case the initial impression of metastic malignancy was countered by more than 2 years' observation, during which time the lesions appeared to be benign. Histologic examination is necessary to exclude malignancy, although a definitive diagnosis may be difficult to establish.", "contents": "Multiple chronic benign pulmonary nodules. Four cases are discussed in which were found unusual multiple chronic pulmonary nodules: leiomyomatous hamartomas, rheumatoid nodules, multiple histoplasmomas, and possible multiple plasma cell granulomas (hyalinizing pulmonary nodules). In each case the initial impression of metastic malignancy was countered by more than 2 years' observation, during which time the lesions appeared to be benign. Histologic examination is necessary to exclude malignancy, although a definitive diagnosis may be difficult to establish.", "PMID": 981596}
{"id": "pubmed23n0032_12634", "title": "Roentgenographic demonstration of peripheral intrapulmonic lymphadenopathy.", "content": "The authors report a case of intrapulmonic lymphadenopathy. While this entity is not common, it should be considered in the differential diagnosis of a solitary pulmonary nodule.", "contents": "Roentgenographic demonstration of peripheral intrapulmonic lymphadenopathy. The authors report a case of intrapulmonic lymphadenopathy. While this entity is not common, it should be considered in the differential diagnosis of a solitary pulmonary nodule.", "PMID": 981597}
{"id": "pubmed23n0032_12635", "title": "Improved cleansing for colonic radiography.", "content": "A well tolerated regimen utilizing Phosphosoda and X-Prep Liquid effectively cleanses the bowel prior to x-ray examination. Good to excellent visualization was achieved in 89.3 per cent of the patients involved in the study.", "contents": "Improved cleansing for colonic radiography. A well tolerated regimen utilizing Phosphosoda and X-Prep Liquid effectively cleanses the bowel prior to x-ray examination. Good to excellent visualization was achieved in 89.3 per cent of the patients involved in the study.", "PMID": 981592}
{"id": "pubmed23n0032_12636", "title": "Radiographic manifestations of pulmonary histoplasmosis: a 10-year review.", "content": "Of 269 patients with histoplasmosis, 259 had radiographic manifestations of the disease. Of these, 217 showed multiple or solitary nodules, 28 infiltrates, 25 adenopathy, 5 fibrosing mediastinitis, 5 cavitation (3 infiltrates, 2 nodules), and 1 pleural effusion.", "contents": "Radiographic manifestations of pulmonary histoplasmosis: a 10-year review. Of 269 patients with histoplasmosis, 259 had radiographic manifestations of the disease. Of these, 217 showed multiple or solitary nodules, 28 infiltrates, 25 adenopathy, 5 fibrosing mediastinitis, 5 cavitation (3 infiltrates, 2 nodules), and 1 pleural effusion.", "PMID": 981598}
{"id": "pubmed23n0032_12637", "title": "Establishing two-year college affiliated programs in radiologic technology.", "content": "The practical considerations of instituting programs in radiologic technology at the two-year college levels are addressed. The authors attempt to identify a \"check-list\" of the areas that will be of vital concern to the prospective program director, including some specific exhibits relating to program implementation and operation.", "contents": "Establishing two-year college affiliated programs in radiologic technology. The practical considerations of instituting programs in radiologic technology at the two-year college levels are addressed. The authors attempt to identify a \"check-list\" of the areas that will be of vital concern to the prospective program director, including some specific exhibits relating to program implementation and operation.", "PMID": 981593}
{"id": "pubmed23n0032_12638", "title": "Twenty degree angle modification for pelvimetry.", "content": "The standard Colcher-Sussman pelvimetry technique does not adequately visualize the interischial distance on a significant number of patients. A twenty-degree angulation modification has been developed to solve this problem.", "contents": "Twenty degree angle modification for pelvimetry. The standard Colcher-Sussman pelvimetry technique does not adequately visualize the interischial distance on a significant number of patients. A twenty-degree angulation modification has been developed to solve this problem.", "PMID": 981594}
{"id": "pubmed23n0032_12639", "title": "Unusual noninfectious granulomas of the lung.", "content": "Illustrative cases of pulmonary granulomas with angitis and plasma cell granuloma of the lungs are presented. Experimental evidence suggests that the common pathogenesis of these processes is a cell-mediated immune response. Radiographic findings of these entities--nodules or infiltrates, some with cavities--are non-specific. Effective treatment is available for some of these processes. Lung biopsy as early as possible is recommended.", "contents": "Unusual noninfectious granulomas of the lung. Illustrative cases of pulmonary granulomas with angitis and plasma cell granuloma of the lungs are presented. Experimental evidence suggests that the common pathogenesis of these processes is a cell-mediated immune response. Radiographic findings of these entities--nodules or infiltrates, some with cavities--are non-specific. Effective treatment is available for some of these processes. Lung biopsy as early as possible is recommended.", "PMID": 981599}
{"id": "pubmed23n0032_12640", "title": "The round nipple shadow.", "content": "It is usually easy to distinguish the round nipple shadow from a lesion, especially by means of repeat radiographs with nipple markers. However, the radiologist must occasionally interpret a round density in the lower chest on the basis of a single radiograph or series of radiographs. Fortunately, a nipple shadow usually has a well-defined outer margin and a poorly defined inner margin. Differentiation of a nipple shadow from an extrapleural or pleural lesion is described. Attention to these findings may prevent needless additional study and patient apprehension.", "contents": "The round nipple shadow. It is usually easy to distinguish the round nipple shadow from a lesion, especially by means of repeat radiographs with nipple markers. However, the radiologist must occasionally interpret a round density in the lower chest on the basis of a single radiograph or series of radiographs. Fortunately, a nipple shadow usually has a well-defined outer margin and a poorly defined inner margin. Differentiation of a nipple shadow from an extrapleural or pleural lesion is described. Attention to these findings may prevent needless additional study and patient apprehension.", "PMID": 981600}
{"id": "pubmed23n0032_12641", "title": "The management of radiological renovation.", "content": "Planning a radiology facility within existing space often creates compromises. A means of measuring the extent of compromise is presented together with an overview of the design process and advice on construction processes.", "contents": "The management of radiological renovation. Planning a radiology facility within existing space often creates compromises. A means of measuring the extent of compromise is presented together with an overview of the design process and advice on construction processes.", "PMID": 981595}
{"id": "pubmed23n0032_12642", "title": "Portal hypertension changes following selective splenorenal shunt surgery. Evaluation by percutaneous transhepatic portal catheterization, venography, and cinefluorography.", "content": "Considerable diagnostic data can be gathered by selectively catheterizing the portal vein and the major tributaries of the splanchnic venous system to evaluate pressure and blood flow. Combined with cinefluorography, this is a superior method for demonstrating the anatomy and hemodynamics of portal hypertension as well as the effects of splenorenal shunt surgery. Thirty-one attempts with a 19 gauge trocar in 17 patients resulted in 28 successful portal entries and 24 successful selective catheterizations. Portal vein pressure, flow, and diameter were less after shunting.", "contents": "Portal hypertension changes following selective splenorenal shunt surgery. Evaluation by percutaneous transhepatic portal catheterization, venography, and cinefluorography. Considerable diagnostic data can be gathered by selectively catheterizing the portal vein and the major tributaries of the splanchnic venous system to evaluate pressure and blood flow. Combined with cinefluorography, this is a superior method for demonstrating the anatomy and hemodynamics of portal hypertension as well as the effects of splenorenal shunt surgery. Thirty-one attempts with a 19 gauge trocar in 17 patients resulted in 28 successful portal entries and 24 successful selective catheterizations. Portal vein pressure, flow, and diameter were less after shunting.", "PMID": 981601}
{"id": "pubmed23n0032_12643", "title": "Whole-gut irrigation as a means of cleaning the colon.", "content": "The authors describe a whole-gut irrigation method of cleaning the colon. Normal volunteers drank an irrigant solution at varying rates, and all but one achieved a radiologically clean colon within several hours. This method is proposed for use in the occasional study where a clean colon must be obtained in a relatively short period of time.", "contents": "Whole-gut irrigation as a means of cleaning the colon. The authors describe a whole-gut irrigation method of cleaning the colon. Normal volunteers drank an irrigant solution at varying rates, and all but one achieved a radiologically clean colon within several hours. This method is proposed for use in the occasional study where a clean colon must be obtained in a relatively short period of time.", "PMID": 981602}
{"id": "pubmed23n0032_12644", "title": "Demonstration of dilated biliary ducts by total-body opacification. Differentiation of surgical from nonsurgical jaundice.", "content": "Differentiation of surgical from medical jaundice remains a difficult clinical problem. Dilated biliary ducts can be demonstrated by total-body opacification (TBO) and their typical appearance is presented. This suggests that TBO may represent a noninvasive adjunct in distinguishing surgical from medical jaundice.", "contents": "Demonstration of dilated biliary ducts by total-body opacification. Differentiation of surgical from nonsurgical jaundice. Differentiation of surgical from medical jaundice remains a difficult clinical problem. Dilated biliary ducts can be demonstrated by total-body opacification (TBO) and their typical appearance is presented. This suggests that TBO may represent a noninvasive adjunct in distinguishing surgical from medical jaundice.", "PMID": 981603}
{"id": "pubmed23n0032_12645", "title": "A solitary calyx in a human kidney.", "content": "A solitary kidney containing a single calyx and probably a single papilla is described. The embryology of this entity and its similarity to the unipapillary kidney in other mammals are discussed.", "contents": "A solitary calyx in a human kidney. A solitary kidney containing a single calyx and probably a single papilla is described. The embryology of this entity and its similarity to the unipapillary kidney in other mammals are discussed.", "PMID": 981604}
{"id": "pubmed23n0032_12646", "title": "The significance of pancreatic ductal obstruction in differential diagnosis of the abnormal endoscopic retrograde pancreatogram.", "content": "Apparent obstruction of the main pancreatic duct was seen on the endoscopic retrograde pancreatogram in 50 patients. The final diagnosis was pseudocyst in 15, neoplasm in 15, chronic pancreatitis in 8, acute recurrent pancreatitis in 3, and abscess in 2. Seven patients were normal. This series emphasizes the broad differential approach necessary when confronted with ductal obstruction. Diagnosis is facilitated by accurate evaluation of the ductal and extraductal characteristics, especially the ductal detail at the point of termination.", "contents": "The significance of pancreatic ductal obstruction in differential diagnosis of the abnormal endoscopic retrograde pancreatogram. Apparent obstruction of the main pancreatic duct was seen on the endoscopic retrograde pancreatogram in 50 patients. The final diagnosis was pseudocyst in 15, neoplasm in 15, chronic pancreatitis in 8, acute recurrent pancreatitis in 3, and abscess in 2. Seven patients were normal. This series emphasizes the broad differential approach necessary when confronted with ductal obstruction. Diagnosis is facilitated by accurate evaluation of the ductal and extraductal characteristics, especially the ductal detail at the point of termination.", "PMID": 981605}
{"id": "pubmed23n0032_12647", "title": "Arteriographic diagnosis of a gunshot injury to the ureter.", "content": "A gunshot wound to the ureter was diagnosed anigiographically. Such injuries are not common; however, they are frequent enough and the consequences of delayed diagnosis and secondary repair are serious enough that ureteric injury should be considered in all cases of abdominal trauma.", "contents": "Arteriographic diagnosis of a gunshot injury to the ureter. A gunshot wound to the ureter was diagnosed anigiographically. Such injuries are not common; however, they are frequent enough and the consequences of delayed diagnosis and secondary repair are serious enough that ureteric injury should be considered in all cases of abdominal trauma.", "PMID": 981606}
{"id": "pubmed23n0032_12648", "title": "Intraluminal obliteration of the renal arteries in kidney tumors.", "content": "A preoperative obliteration of the renal vascular bed was performed in 90 patients by means of Gelfoam emboli. This procedure facilitates the ligation of the renal vein and artery during transabdominal nephrectomy. The decreased vascularity lessens the risk of tumor embolization and reduces the duration of the surgical procedure by half. A simple routine percutaneous method of occlusion is described. Gelfoam emboli are unsuited for permanent obliteration in bleeding tumors.", "contents": "Intraluminal obliteration of the renal arteries in kidney tumors. A preoperative obliteration of the renal vascular bed was performed in 90 patients by means of Gelfoam emboli. This procedure facilitates the ligation of the renal vein and artery during transabdominal nephrectomy. The decreased vascularity lessens the risk of tumor embolization and reduces the duration of the surgical procedure by half. A simple routine percutaneous method of occlusion is described. Gelfoam emboli are unsuited for permanent obliteration in bleeding tumors.", "PMID": 981607}
{"id": "pubmed23n0032_12649", "title": "Clinicopathological correlations of xeroradiography in determining involvement of metastic axillary nodes in female breast cancer.", "content": "Xeroradiographic evaluation of the axilla is useful in the presence of mammary carcinoma, as radiographic examination of this area can be highly accurate in predicting its malignant involvement if certain strict criteria are employed. The ability to diagnose nodal metastases with near certainty has important implications for treatment planning and prognostication.", "contents": "Clinicopathological correlations of xeroradiography in determining involvement of metastic axillary nodes in female breast cancer. Xeroradiographic evaluation of the axilla is useful in the presence of mammary carcinoma, as radiographic examination of this area can be highly accurate in predicting its malignant involvement if certain strict criteria are employed. The ability to diagnose nodal metastases with near certainty has important implications for treatment planning and prognostication.", "PMID": 981609}
{"id": "pubmed23n0032_12650", "title": "Observer performance in detecting multiple radiographic signals. Prediction and analysis using a generalized ROC approach.", "content": "The theories of decision processes and signal detection provide a framework for evaluation of observer performance. Some radiological procedures involve a search for multiple similar lesions, e.g., plain radiographic examinations for gallstones or pneumoconiosis. Presuming knowledge of the conventional ROC curve for detection of a single radiographic signal, a model is presented which is used to predict observer performance in an experiment requiring detection of more than one such signal. An experiment tests the validity of this model for detecting radiographically the presence of zero, one, or two low-contrast, 2-mm diameter, Lucite beads. Results confirm the validity of the model and suggest that observer performance in relatively complex detection tasks can be predicted from simpler experiments.", "contents": "Observer performance in detecting multiple radiographic signals. Prediction and analysis using a generalized ROC approach. The theories of decision processes and signal detection provide a framework for evaluation of observer performance. Some radiological procedures involve a search for multiple similar lesions, e.g., plain radiographic examinations for gallstones or pneumoconiosis. Presuming knowledge of the conventional ROC curve for detection of a single radiographic signal, a model is presented which is used to predict observer performance in an experiment requiring detection of more than one such signal. An experiment tests the validity of this model for detecting radiographically the presence of zero, one, or two low-contrast, 2-mm diameter, Lucite beads. Results confirm the validity of the model and suggest that observer performance in relatively complex detection tasks can be predicted from simpler experiments.", "PMID": 981610}
{"id": "pubmed23n0032_12651", "title": "Clinical evaluation of the rapid telephone access system for radiology reporting.", "content": "The Rapid Telephone Access System (RTAS) for radiology reporting was evaluated clinically among patients undergoing fluoroscopy, excretory urography, or cholecystography at a community-based teaching hospital. After the radiologist has dictated his report in the customary manner, it can be heard by the referring physician using any telephone. Reports can be reduced to hard copy by a typist, or an automatic typewriter can be programmed to print normal or standard reports. Time between dictation and access of the report was significantly reduced after installation of the RTAS (p less than 0.001). There was also a trend toward reduced hospital stay (p less than 0.05) for patients in the study group. The system has been readily accepted by radiologists and referring physicians.", "contents": "Clinical evaluation of the rapid telephone access system for radiology reporting. The Rapid Telephone Access System (RTAS) for radiology reporting was evaluated clinically among patients undergoing fluoroscopy, excretory urography, or cholecystography at a community-based teaching hospital. After the radiologist has dictated his report in the customary manner, it can be heard by the referring physician using any telephone. Reports can be reduced to hard copy by a typist, or an automatic typewriter can be programmed to print normal or standard reports. Time between dictation and access of the report was significantly reduced after installation of the RTAS (p less than 0.001). There was also a trend toward reduced hospital stay (p less than 0.05) for patients in the study group. The system has been readily accepted by radiologists and referring physicians.", "PMID": 981611}
{"id": "pubmed23n0032_12652", "title": "Diagnostic follow-up to consultant physicians by computer feedback.", "content": "A computer follow-up system has been developed which automatically prepares follow-up summaries for consultant physicians. This makes it possible to provide reliable feedback of diagnostic information in place of the traditional but haphazard word-of-mouth process. This system has been tested initially for surgical and autopsy-pathology follow-up of neuroradiology and nuclear medicine consultations using report conclusions available from the UCLA Natural Language Information Storage and Retrieval System.", "contents": "Diagnostic follow-up to consultant physicians by computer feedback. A computer follow-up system has been developed which automatically prepares follow-up summaries for consultant physicians. This makes it possible to provide reliable feedback of diagnostic information in place of the traditional but haphazard word-of-mouth process. This system has been tested initially for surgical and autopsy-pathology follow-up of neuroradiology and nuclear medicine consultations using report conclusions available from the UCLA Natural Language Information Storage and Retrieval System.", "PMID": 981612}
{"id": "pubmed23n0032_12653", "title": "Effectiveness of lead lenses in reducing radiation exposure.", "content": "Optically ground prescription lenses of glass, leaded glass, and plastic were exposed to radiations that simulated routine angiography. Radiations transmitted through the lenses were measured. Plastic provided no protection, regular glass provided moderate protection, and high lead content glass reduced radiation transmission by approximately 70%. A brief review of the literature concerning the biological effects of radiations to the eye is included.", "contents": "Effectiveness of lead lenses in reducing radiation exposure. Optically ground prescription lenses of glass, leaded glass, and plastic were exposed to radiations that simulated routine angiography. Radiations transmitted through the lenses were measured. Plastic provided no protection, regular glass provided moderate protection, and high lead content glass reduced radiation transmission by approximately 70%. A brief review of the literature concerning the biological effects of radiations to the eye is included.", "PMID": 981613}
{"id": "pubmed23n0032_12654", "title": "Computed tomography of the spinal canal.", "content": "The value of computed tomography (CT) in cases of spinal dysraphism, spinal canal narrowing and widening, and spinal bony abnormalities is discussed. With further experience, CT may become a valuable modality for the diagnosis of spinal diseases.", "contents": "Computed tomography of the spinal canal. The value of computed tomography (CT) in cases of spinal dysraphism, spinal canal narrowing and widening, and spinal bony abnormalities is discussed. With further experience, CT may become a valuable modality for the diagnosis of spinal diseases.", "PMID": 981614}
{"id": "pubmed23n0032_12655", "title": "Recognition of vertebral abnormalities in computed tomography of the chest and abdomen.", "content": "Computed tomography of the chest or abdomen may reveal vertebral abnormalities. Images in multiple projections of dry and fresh specimens of the spine demonstrate that osteophytes, end-plate irregularities, and marked obliquity fail to mimic true osteoblastic vertebral lesions. Disk calcification may simulate a metastatic deposit if the section includes both disk and vertebral body. Abdominal or chest \"window\" settings produce an apparent increase in bone density. Analysis of the bony spine from abdominal and chest images requires gray scale settings different from those used for the study of visceral organs.", "contents": "Recognition of vertebral abnormalities in computed tomography of the chest and abdomen. Computed tomography of the chest or abdomen may reveal vertebral abnormalities. Images in multiple projections of dry and fresh specimens of the spine demonstrate that osteophytes, end-plate irregularities, and marked obliquity fail to mimic true osteoblastic vertebral lesions. Disk calcification may simulate a metastatic deposit if the section includes both disk and vertebral body. Abdominal or chest \"window\" settings produce an apparent increase in bone density. Analysis of the bony spine from abdominal and chest images requires gray scale settings different from those used for the study of visceral organs.", "PMID": 981615}
{"id": "pubmed23n0032_12656", "title": "Extravascular contrast material: the major component of contrast enhancement.", "content": "The distribution volume of 125l-diatrizoate in rat tissues was studied 5 seconds to 5 minutes after intravenous injection and 5 seconds to 2 minutes after intra-arterial injection. Immediately after injection, diatrizoate was distributed into a larger volume than plasma. Marked temporal changes in the distribution volume of individual tissues occurred during the first 2 minutes. At 5 minutes, the diatrizoate space approached the total extracellular fluid volume, with more than 80% of the contrast agent remaining outside the blood vessels. Thus the relative magnitude of contrast enhancement of a tissue appears to be related to the volume of the rapidly equilibrating extracellular space. Intra-arterial administration can significantly overload muscle with contrast material for about 2 minutes. This and other temporal changes in contrast distribution may prove useful in contrast-enhanced computed tomography using short scanning times.", "contents": "Extravascular contrast material: the major component of contrast enhancement. The distribution volume of 125l-diatrizoate in rat tissues was studied 5 seconds to 5 minutes after intravenous injection and 5 seconds to 2 minutes after intra-arterial injection. Immediately after injection, diatrizoate was distributed into a larger volume than plasma. Marked temporal changes in the distribution volume of individual tissues occurred during the first 2 minutes. At 5 minutes, the diatrizoate space approached the total extracellular fluid volume, with more than 80% of the contrast agent remaining outside the blood vessels. Thus the relative magnitude of contrast enhancement of a tissue appears to be related to the volume of the rapidly equilibrating extracellular space. Intra-arterial administration can significantly overload muscle with contrast material for about 2 minutes. This and other temporal changes in contrast distribution may prove useful in contrast-enhanced computed tomography using short scanning times.", "PMID": 981616}
{"id": "pubmed23n0032_12657", "title": "Unusual pelvic masses: a comparison of computed tomographic scanning and ultrasonography.", "content": "Four unusual pelvic masses, studied by CT and gray scale ultrasonography, demonstrate the comparative values of these modalities for this area of the body. In each instance a significant contribution to the diagnosis was made by one or both techniques. The ultrasound study provides a somewhat better differentiation of solid from cystic lesions, but the CT scan denotes to best advantage the anatomy of the area, and provides a means of viewing bone as well as soft tissue detail.", "contents": "Unusual pelvic masses: a comparison of computed tomographic scanning and ultrasonography. Four unusual pelvic masses, studied by CT and gray scale ultrasonography, demonstrate the comparative values of these modalities for this area of the body. In each instance a significant contribution to the diagnosis was made by one or both techniques. The ultrasound study provides a somewhat better differentiation of solid from cystic lesions, but the CT scan denotes to best advantage the anatomy of the area, and provides a means of viewing bone as well as soft tissue detail.", "PMID": 981617}
{"id": "pubmed23n0032_12658", "title": "Cephalic malformations in Saethre-Chotzen syndrome. Acrocephalosyndactyly type III.", "content": "Specific anomalies of the skull in eight family members affected with Saethre-Chotzen syndrome were documented by cephalometric analysis of standardized skull radiographs. The most severe deviations included reduced length and abnormal position of the posterior cranial base, low position of the sella turcica, reduced mandibular ramus length, steep mandibular plane angle, and, in adults, reduced facial depth. A feature of the syndrome may be the absence or reduced size of cranial sinuses.", "contents": "Cephalic malformations in Saethre-Chotzen syndrome. Acrocephalosyndactyly type III. Specific anomalies of the skull in eight family members affected with Saethre-Chotzen syndrome were documented by cephalometric analysis of standardized skull radiographs. The most severe deviations included reduced length and abnormal position of the posterior cranial base, low position of the sella turcica, reduced mandibular ramus length, steep mandibular plane angle, and, in adults, reduced facial depth. A feature of the syndrome may be the absence or reduced size of cranial sinuses.", "PMID": 981618}
{"id": "pubmed23n0032_12659", "title": "The Mark IV system for radionuclide computed tomography of the brain.", "content": "The Mark IV scanning system is a simple four-sided arrangement of 32 independent detectors which rotate continously as a unit, detecting, processing, and displaying the reconstructed data while the study progresses. Detection is by single photon counting and is compatible with commercially available radionuclides. An empirical correction is applied for attenuation, difference in detector response, and scatter. It is a high-sensitivity device with approximately uniform resolution throughout the section plane.  There is good reproducibility and accuracy for absolute quantification of radionuclide concentration in the brain.  Clinical applications include scans of 99mTcO4, 99mTc-RBC, 123I-iodoantipyrine, 99mTc-diphosphonate, and 111In-DTPA.", "contents": "The Mark IV system for radionuclide computed tomography of the brain. The Mark IV scanning system is a simple four-sided arrangement of 32 independent detectors which rotate continously as a unit, detecting, processing, and displaying the reconstructed data while the study progresses. Detection is by single photon counting and is compatible with commercially available radionuclides. An empirical correction is applied for attenuation, difference in detector response, and scatter. It is a high-sensitivity device with approximately uniform resolution throughout the section plane.  There is good reproducibility and accuracy for absolute quantification of radionuclide concentration in the brain.  Clinical applications include scans of 99mTcO4, 99mTc-RBC, 123I-iodoantipyrine, 99mTc-diphosphonate, and 111In-DTPA.", "PMID": 981619}
{"id": "pubmed23n0032_12660", "title": "Pulmonary retention and translocation of insufflated tantalum.", "content": "Studies of pylmonary clearance of tantalum dust labeled with 182Ta in dogs have been extended to as far as 816 days postexposure in order to substantiate earlier pulmonary retention data, examine lymphatic uptake, and seek long-term pathological changes. Exposure to 5-mug and 10-mug powders was compared with the results of earlier 1-mug studies. The 2.1-year long-term clearance half-time of 1-mug powder was confirmed, while the 5-mug and 10-mug powders were found to have a mean long-term retention half-time of 333 days. All three particle sizes demonstrated rapid postinsufflation uptake by the pulmonary lymph nodes, followed by either retention or very slow clearance. No tantalum-induced pathological changes were found on histological examination.", "contents": "Pulmonary retention and translocation of insufflated tantalum. Studies of pylmonary clearance of tantalum dust labeled with 182Ta in dogs have been extended to as far as 816 days postexposure in order to substantiate earlier pulmonary retention data, examine lymphatic uptake, and seek long-term pathological changes. Exposure to 5-mug and 10-mug powders was compared with the results of earlier 1-mug studies. The 2.1-year long-term clearance half-time of 1-mug powder was confirmed, while the 5-mug and 10-mug powders were found to have a mean long-term retention half-time of 333 days. All three particle sizes demonstrated rapid postinsufflation uptake by the pulmonary lymph nodes, followed by either retention or very slow clearance. No tantalum-induced pathological changes were found on histological examination.", "PMID": 981620}
{"id": "pubmed23n0032_12661", "title": "Ventilation-perfusion lung images in the Swyer-James syndrome.", "content": "Ventilation-perfusion lung imaging appears to be a significant nonivasive means of supporting the clinical diagnosis of Swyer-James syndrome. Three cases are presented which display characteristic delayed washout of xenon from a small or normal-sized, underperfused hyperlucent lung.", "contents": "Ventilation-perfusion lung images in the Swyer-James syndrome. Ventilation-perfusion lung imaging appears to be a significant nonivasive means of supporting the clinical diagnosis of Swyer-James syndrome. Three cases are presented which display characteristic delayed washout of xenon from a small or normal-sized, underperfused hyperlucent lung.", "PMID": 981621}
{"id": "pubmed23n0032_12662", "title": "Comparison of enzyme, clinical, radiographic, and radionuclide methods of detecting bone metastases from carcinoma of the prostate.", "content": "Patients (219) with prostatic adenocarcinoma were classified on the basis of whether or not their bone scans were positive for metastasis. Acid and alkaline phosphatase determinations and clinical evaluations for bone metastases were reviewed. Of those with proved metastases, 43% had no bone pain, 39% had normal acid phosphatase levels, 23% normal alkaline phosphatase levels, 19% normal levels of both enzymes, and 15% normal enzyme levels without bone pain. Twenty-four per cent of the patients with normal enzyme levels and clinically unsuspected bone metastases had bone scans which proved positive for metastasis; 62% of these had normal radiographs.", "contents": "Comparison of enzyme, clinical, radiographic, and radionuclide methods of detecting bone metastases from carcinoma of the prostate. Patients (219) with prostatic adenocarcinoma were classified on the basis of whether or not their bone scans were positive for metastasis. Acid and alkaline phosphatase determinations and clinical evaluations for bone metastases were reviewed. Of those with proved metastases, 43% had no bone pain, 39% had normal acid phosphatase levels, 23% normal alkaline phosphatase levels, 19% normal levels of both enzymes, and 15% normal enzyme levels without bone pain. Twenty-four per cent of the patients with normal enzyme levels and clinically unsuspected bone metastases had bone scans which proved positive for metastasis; 62% of these had normal radiographs.", "PMID": 981622}
{"id": "pubmed23n0032_12663", "title": "Cystic hamartoma of the liver in a pediatric patient. Angiographic findings.", "content": "A case of cystic mesenchymal hamartoma in a 2-year-old boy is presented. The overall angiographic appearance closely resembled that of the more common renal angiomyolipoma, with the addition of multiple focal avascular areas representing the cystic component of the lesion. The coexistence of these angiographic findings in a young patient with an otherwise asymptomatic upper abdominal mass should suggest cystic mesenchymal hamartoma.", "contents": "Cystic hamartoma of the liver in a pediatric patient. Angiographic findings. A case of cystic mesenchymal hamartoma in a 2-year-old boy is presented. The overall angiographic appearance closely resembled that of the more common renal angiomyolipoma, with the addition of multiple focal avascular areas representing the cystic component of the lesion. The coexistence of these angiographic findings in a young patient with an otherwise asymptomatic upper abdominal mass should suggest cystic mesenchymal hamartoma.", "PMID": 981623}
{"id": "pubmed23n0032_12664", "title": "Juvenile gastrointestinal polyposis.", "content": "Two cases of juvenile gastrointestinal polyposis are described and the literature is reviewed. Despite the benign nature of the individual polyps, the disease has a poor prognosis. Of 13 reported patients, 4 had macrocephaly, and one of the authors' 2 patients had large peripheral intracranial cysts. This appears to be the first reported documentation of the etiology of macrocephaly associated with juvenile polyposis. Major clinical problems are related to severe loss of blood and protein from the gastrointestinal tract.", "contents": "Juvenile gastrointestinal polyposis. Two cases of juvenile gastrointestinal polyposis are described and the literature is reviewed. Despite the benign nature of the individual polyps, the disease has a poor prognosis. Of 13 reported patients, 4 had macrocephaly, and one of the authors' 2 patients had large peripheral intracranial cysts. This appears to be the first reported documentation of the etiology of macrocephaly associated with juvenile polyposis. Major clinical problems are related to severe loss of blood and protein from the gastrointestinal tract.", "PMID": 981624}
{"id": "pubmed23n0032_12665", "title": "Gray scale ultrasonic cholecystography: a comparison with conventional radiographic techniques.", "content": "A prospective study was designed to assess the accuracy of gray scale ultrasonography in the evaluation of cholelithiasis. A series of 111 patients with nonvisualization on first-day oral cholecystography underwent ultrasonic cholecystography, and the results were compared with subsequent second-day visualitzation or surgical findings. In 75 cases sufficient data were present to assess the accuracy of the method. Ultrasound correctly diagnosed 68 (91%) of these cases with regard to gallstones. Only 6 gallbladders could not be visualized ultrasonically and all subsequently proved to have gallstones. It is concluded that improved ultrasonic technique is of considerable value in investigating the nonvisualized gallbladder. Valuable information conderning other upper abdominal pathology was often discovered.", "contents": "Gray scale ultrasonic cholecystography: a comparison with conventional radiographic techniques. A prospective study was designed to assess the accuracy of gray scale ultrasonography in the evaluation of cholelithiasis. A series of 111 patients with nonvisualization on first-day oral cholecystography underwent ultrasonic cholecystography, and the results were compared with subsequent second-day visualitzation or surgical findings. In 75 cases sufficient data were present to assess the accuracy of the method. Ultrasound correctly diagnosed 68 (91%) of these cases with regard to gallstones. Only 6 gallbladders could not be visualized ultrasonically and all subsequently proved to have gallstones. It is concluded that improved ultrasonic technique is of considerable value in investigating the nonvisualized gallbladder. Valuable information conderning other upper abdominal pathology was often discovered.", "PMID": 981625}
{"id": "pubmed23n0032_12666", "title": "Fetal cystic lymphangioma (cystic hygroma): prepartum ultrasonic findings.", "content": "The abnormal prepartum ultrasonic scan in a case of fetal cystic lymphangioma is presented. A large intrauterine cystic mass adjacent to a 23-week-old fetus was identified by ultrasound. After delivery, this was proved to be a cystic lymphangioma extending from the fetal neck and supraclavicular region.", "contents": "Fetal cystic lymphangioma (cystic hygroma): prepartum ultrasonic findings. The abnormal prepartum ultrasonic scan in a case of fetal cystic lymphangioma is presented. A large intrauterine cystic mass adjacent to a 23-week-old fetus was identified by ultrasound. After delivery, this was proved to be a cystic lymphangioma extending from the fetal neck and supraclavicular region.", "PMID": 981626}
{"id": "pubmed23n0032_12667", "title": "Combinations of hyperthermia (40 degrees, 45 degrees C) with radiation.", "content": "Incubation of Chinese hamster cells at an elevated but sublethal temperature between fractions of radiation and/or pulses of hyperthermia at 45 degrees C strongly modified the effectiveness of cell killing. Increasing the incubation temperature from 37 to 40 degrees C between fractions of hyperthermia at 45 degrees C followed by radiation substantially enhanced cell killing; while the opposite sequence (radiation leads to incubation at 40 degrees C leads to hyperthermia at 45 degrees C) resulted in less effective cell killing than would have been expected from the independent interaction of hyperthermia and radiation alone. This suggests the use of short pulses of localized hyperthermia in the presence of physiologically tolerable fever followed by irradiation as one approach to the utilization of hyperthermia in cancer therapy.", "contents": "Combinations of hyperthermia (40 degrees, 45 degrees C) with radiation. Incubation of Chinese hamster cells at an elevated but sublethal temperature between fractions of radiation and/or pulses of hyperthermia at 45 degrees C strongly modified the effectiveness of cell killing. Increasing the incubation temperature from 37 to 40 degrees C between fractions of hyperthermia at 45 degrees C followed by radiation substantially enhanced cell killing; while the opposite sequence (radiation leads to incubation at 40 degrees C leads to hyperthermia at 45 degrees C) resulted in less effective cell killing than would have been expected from the independent interaction of hyperthermia and radiation alone. This suggests the use of short pulses of localized hyperthermia in the presence of physiologically tolerable fever followed by irradiation as one approach to the utilization of hyperthermia in cancer therapy.", "PMID": 981627}
{"id": "pubmed23n0032_12668", "title": "Hepatic radiation tolerance in Hodgkin's disease patients.", "content": "Prophylactic hepatic irradiation was give to 28 \"bad risk\" Stage III Hodgkin's disease patients. The whole liver was given 2,000 rad in 10 days in addition to 1,800-2,000 rad to that portion included in the total nodal irradiation field. Twenty-three patients have been followed a minimum of 10 months with serial liver function tests. A transient elevation of serum enzymes has been noted at 3-12 months following hepatic irradiation in 78% (18 of 23 patients). No cases of clinical radiation hepatitis have been found in patients at risk 10 to 39 months (median, 23 months). Hepatic irradiation at this dose level appears to be tolerated without prohibitive complications.", "contents": "Hepatic radiation tolerance in Hodgkin's disease patients. Prophylactic hepatic irradiation was give to 28 \"bad risk\" Stage III Hodgkin's disease patients. The whole liver was given 2,000 rad in 10 days in addition to 1,800-2,000 rad to that portion included in the total nodal irradiation field. Twenty-three patients have been followed a minimum of 10 months with serial liver function tests. A transient elevation of serum enzymes has been noted at 3-12 months following hepatic irradiation in 78% (18 of 23 patients). No cases of clinical radiation hepatitis have been found in patients at risk 10 to 39 months (median, 23 months). Hepatic irradiation at this dose level appears to be tolerated without prohibitive complications.", "PMID": 981628}
{"id": "pubmed23n0032_12669", "title": "An evaluation of rare earth screen/film combinations.", "content": "The physical and imaging characteristics of a number of commercially available rare earth screen/film combinations were evaluated and compared to those of conventional calcium tungstate/X-Omat RP-14 systems. All systems were evaluated for base-plus-fog density, relative speed, resolution, noise, and overall performance. The results indicate that despite certain limitations, rare earth screen/film combinations offer significant advantages for imaging.", "contents": "An evaluation of rare earth screen/film combinations. The physical and imaging characteristics of a number of commercially available rare earth screen/film combinations were evaluated and compared to those of conventional calcium tungstate/X-Omat RP-14 systems. All systems were evaluated for base-plus-fog density, relative speed, resolution, noise, and overall performance. The results indicate that despite certain limitations, rare earth screen/film combinations offer significant advantages for imaging.", "PMID": 981629}
{"id": "pubmed23n0032_12670", "title": "The LSF and MTF of rare-earth oxysulfide intensifying screens.", "content": "The line spread function (LSF) and modulation transfer function (MTF) of 9 rare-earth screen/film systems were measured and compared with those of two fast calcium tungstate systems, using double-emulsion films sandwiched between two screens and mounted in regular cassettes. The LSFs were found to fit exponential functions. These results indicate that the increased sensitivity of rare-earth phosphors over calcium tungstate can be used to construct screens with a higher MTF or increased speed. The fast rare-earth systems allow the use of smaller focal spots for increased resolution while reducing the radiation dose to the patient.", "contents": "The LSF and MTF of rare-earth oxysulfide intensifying screens. The line spread function (LSF) and modulation transfer function (MTF) of 9 rare-earth screen/film systems were measured and compared with those of two fast calcium tungstate systems, using double-emulsion films sandwiched between two screens and mounted in regular cassettes. The LSFs were found to fit exponential functions. These results indicate that the increased sensitivity of rare-earth phosphors over calcium tungstate can be used to construct screens with a higher MTF or increased speed. The fast rare-earth systems allow the use of smaller focal spots for increased resolution while reducing the radiation dose to the patient.", "PMID": 981630}
{"id": "pubmed23n0032_12671", "title": "In vitro radiosensitivity of human dipliod fibroblasts derived from patients with unusual clinical responses to radiation.", "content": "Four diploid cell strains were derived from skin biopsies from patients exhibiting unusually sensitive or resistant clinical responses to ionizing radiation during radiotherapy. The in vitro x-ray survival curve parameters for these strains were determined and compared with those of two normal human skin fibroblast strains. No systematic correlation could be demonstrated between these single dose survival parameters in vitro, and the clinical radiation response in vivo of the normal tissue or the tumor.", "contents": "In vitro radiosensitivity of human dipliod fibroblasts derived from patients with unusual clinical responses to radiation. Four diploid cell strains were derived from skin biopsies from patients exhibiting unusually sensitive or resistant clinical responses to ionizing radiation during radiotherapy. The in vitro x-ray survival curve parameters for these strains were determined and compared with those of two normal human skin fibroblast strains. No systematic correlation could be demonstrated between these single dose survival parameters in vitro, and the clinical radiation response in vivo of the normal tissue or the tumor.", "PMID": 981631}
{"id": "pubmed23n0032_12672", "title": "Addition of neutron and gamma-ray fractions for intestinal damage.", "content": "The microcolony assay technique has been used to test the validty of summing equivalent doses per fraction of 14 MeV neutrons and gamma rays for mouse intestinal damage. For a 4-daily fraction schedule, in which the first one or two fractions are given as neutrons and the remainder as gamma rays, combined dose fractions calculated from a 4-fraction schedule of either radiation type alone produce the same level of damage within the limits of accuracy of the experiment.", "contents": "Addition of neutron and gamma-ray fractions for intestinal damage. The microcolony assay technique has been used to test the validty of summing equivalent doses per fraction of 14 MeV neutrons and gamma rays for mouse intestinal damage. For a 4-daily fraction schedule, in which the first one or two fractions are given as neutrons and the remainder as gamma rays, combined dose fractions calculated from a 4-fraction schedule of either radiation type alone produce the same level of damage within the limits of accuracy of the experiment.", "PMID": 981632}
{"id": "pubmed23n0032_12673", "title": "Thallium-201 as a positive indicator for lung neoplasms: preliminary experiments.", "content": "Thallium-201 was given to 71 patients with various pulmonary diseases. Positive uptake was noted in primary malignant neoplasms and also in 6 cases of Hodgkin's lymphoma. No uptake was seen in pulmonary diseases other than active tuberculosis. A disadvantage is the high myocardial accumulation of this radionuclide which impairs proper visualization of paracardiac neoplasms.", "contents": "Thallium-201 as a positive indicator for lung neoplasms: preliminary experiments. Thallium-201 was given to 71 patients with various pulmonary diseases. Positive uptake was noted in primary malignant neoplasms and also in 6 cases of Hodgkin's lymphoma. No uptake was seen in pulmonary diseases other than active tuberculosis. A disadvantage is the high myocardial accumulation of this radionuclide which impairs proper visualization of paracardiac neoplasms.", "PMID": 981633}
{"id": "pubmed23n0032_12674", "title": "Irradiation of advanced head and neck cancer with large daily fractions. Preliminary results of a prospectively randomized clinical trial.", "content": "The irradiation of locally advanced, inoperable, squamous cell caricinomas of the head and neck with conventional fractionation schedules using 200 rads/day has produced poor results. A prospectively randomized, clinical trial has been started comparing conventional with large daily fractions of 400 rads/day for 10-12 treatments. Results suggest that improved tumor control and survival rates might be obtained with the use of large daily fractions.", "contents": "Irradiation of advanced head and neck cancer with large daily fractions. Preliminary results of a prospectively randomized clinical trial. The irradiation of locally advanced, inoperable, squamous cell caricinomas of the head and neck with conventional fractionation schedules using 200 rads/day has produced poor results. A prospectively randomized, clinical trial has been started comparing conventional with large daily fractions of 400 rads/day for 10-12 treatments. Results suggest that improved tumor control and survival rates might be obtained with the use of large daily fractions.", "PMID": 981634}
{"id": "pubmed23n0032_12675", "title": "Radiographic visualization of cervix and vagina during intracavitary radium therapy.", "content": "Dry barium-impregnated vaginal packing used in several hundred intracavitary uterine radium insertions has allowed excellent radiographic visualization of the relationship of the radium implant to both the cervical and vaginal walls.", "contents": "Radiographic visualization of cervix and vagina during intracavitary radium therapy. Dry barium-impregnated vaginal packing used in several hundred intracavitary uterine radium insertions has allowed excellent radiographic visualization of the relationship of the radium implant to both the cervical and vaginal walls.", "PMID": 981635}
{"id": "pubmed23n0032_12676", "title": "Lung involvement in angioimmunoblastic lymphadenopathy (ail).", "content": "Two patients with angioimmunoblastic lymphadenopathy (AIL) in whom the disease process involved the lungs are reported. One patient died in respiratory failure. Details of the radiologic appearance, abnormalities of lung function and lung histology are presented together with response to steroid therapy in one patient. Life-threatening involvement of the lung parenchyma can be a mode of presentation of the disease and can contribute to the morbidity and mortality of the disorder.", "contents": "Lung involvement in angioimmunoblastic lymphadenopathy (ail). Two patients with angioimmunoblastic lymphadenopathy (AIL) in whom the disease process involved the lungs are reported. One patient died in respiratory failure. Details of the radiologic appearance, abnormalities of lung function and lung histology are presented together with response to steroid therapy in one patient. Life-threatening involvement of the lung parenchyma can be a mode of presentation of the disease and can contribute to the morbidity and mortality of the disorder.", "PMID": 981637}
{"id": "pubmed23n0032_12677", "title": "The midsternal stripe: a sign of dehiscence following median sternotomy.", "content": "Sternal dehiscence is a recognized complication of median sternotomy in 2.5-4.8% of patients. The authors describe the prognostic significance of a lucent midsternal stripe which was seen in 12 patients over a two-year period. Sternal dehiscence requiring surgical revision developed in 4, and radiological suspicion preceded clinical evidence of dehiscence in 3 of them. A review of 100 consecutive median sternotomies revealed that sternal dehiscence did not develop in any patient who did not have a midsternal stripe. It is suggested that this may be a useful tool in identifying those patients who are at high risk of the development of sternal dehiscence.", "contents": "The midsternal stripe: a sign of dehiscence following median sternotomy. Sternal dehiscence is a recognized complication of median sternotomy in 2.5-4.8% of patients. The authors describe the prognostic significance of a lucent midsternal stripe which was seen in 12 patients over a two-year period. Sternal dehiscence requiring surgical revision developed in 4, and radiological suspicion preceded clinical evidence of dehiscence in 3 of them. A review of 100 consecutive median sternotomies revealed that sternal dehiscence did not develop in any patient who did not have a midsternal stripe. It is suggested that this may be a useful tool in identifying those patients who are at high risk of the development of sternal dehiscence.", "PMID": 981638}
{"id": "pubmed23n0032_12678", "title": "The midsternal stripe and its relationship to postoperative sternal dehiscence.", "content": "The postoperative chest radiographs of 173 patients who underwent median sternternal line. A total of 52 (30%) showed a thin lucent line one or more days after the operation. Clinical dehiscence developed in 4 patients, of whom 2 had a midsternal stripe and 2 did not. The authors feel that the presence of a sternal stripe is of little value in the radiographic diagnosis of sternal dehiscence.", "contents": "The midsternal stripe and its relationship to postoperative sternal dehiscence. The postoperative chest radiographs of 173 patients who underwent median sternternal line. A total of 52 (30%) showed a thin lucent line one or more days after the operation. Clinical dehiscence developed in 4 patients, of whom 2 had a midsternal stripe and 2 did not. The authors feel that the presence of a sternal stripe is of little value in the radiographic diagnosis of sternal dehiscence.", "PMID": 981639}
{"id": "pubmed23n0032_12679", "title": "Bronchial web diagnosed by bronchography.", "content": "Bronchial webs are rare lesions which often go unrecognized. The authors describe a patient who presented with right-lower-lobe bronchiectasis and at-electasis secondary to a web in the right-lower-lobe bronchus. This is the first known instance where such a lesion has been demonstrated by bronchography. The etiology of this and similar lesions reported in the literature remains obscure.", "contents": "Bronchial web diagnosed by bronchography. Bronchial webs are rare lesions which often go unrecognized. The authors describe a patient who presented with right-lower-lobe bronchiectasis and at-electasis secondary to a web in the right-lower-lobe bronchus. This is the first known instance where such a lesion has been demonstrated by bronchography. The etiology of this and similar lesions reported in the literature remains obscure.", "PMID": 981640}
{"id": "pubmed23n0032_12680", "title": "Fluoroscopic axialography: clinical applications in thoracic disease.", "content": "A simple, single-stage, quasi-tomographic technique which is employable with ordinary C-arm fluoroscopes is presented as an alternative to small field chest tomography. Fluoroscopic axialography utilizes image intensifier filming during axial rotation to obtain sharp tomogram-like images of areas of interest. The technique incorporates preliminary fluoroscopic localization and has many applications in the chest. The use of axial rather than planar blurring and intensifier camera filming rather than radiography does not reduce the clinical usefulness of the method.", "contents": "Fluoroscopic axialography: clinical applications in thoracic disease. A simple, single-stage, quasi-tomographic technique which is employable with ordinary C-arm fluoroscopes is presented as an alternative to small field chest tomography. Fluoroscopic axialography utilizes image intensifier filming during axial rotation to obtain sharp tomogram-like images of areas of interest. The technique incorporates preliminary fluoroscopic localization and has many applications in the chest. The use of axial rather than planar blurring and intensifier camera filming rather than radiography does not reduce the clinical usefulness of the method.", "PMID": 981641}
{"id": "pubmed23n0032_12681", "title": "Thickening of the posterior tracheal stripe: a sign of squamous cell carcinoma of the esophagus.", "content": "A thickened posterior tracheal stripe (wider than 4.5 mm) was identified on the lateral check radiographs of 20 patients with squamous cell carcinoma of the esophagus. Autopsy studies confirmed that peri-esophageal lymphatic involvement was responsible for the thickening of the stripe in the nonobstructed esophagus. This sign appeared on the lateral chest radiograph as early as 6 months prior to the development of symptoms in 50% of the cases studied.", "contents": "Thickening of the posterior tracheal stripe: a sign of squamous cell carcinoma of the esophagus. A thickened posterior tracheal stripe (wider than 4.5 mm) was identified on the lateral check radiographs of 20 patients with squamous cell carcinoma of the esophagus. Autopsy studies confirmed that peri-esophageal lymphatic involvement was responsible for the thickening of the stripe in the nonobstructed esophagus. This sign appeared on the lateral chest radiograph as early as 6 months prior to the development of symptoms in 50% of the cases studied.", "PMID": 981642}
{"id": "pubmed23n0032_12682", "title": "The effect of valium on myocardial blood flow.", "content": "The effect of Valium on coronary blood flow was studied. In 18 patients undergoing coronary angiography, total and regional myocardial blood flow was determined by the 133Xe wash-out technique. A total of 39 pre- and 39 post-Valium injection wash-out curves were constructed. Results indicate (99% confidence) that intravenous Valium injection (0.1 mg/kg body weight) decreases myocardial blood flow 12--15%.", "contents": "The effect of valium on myocardial blood flow. The effect of Valium on coronary blood flow was studied. In 18 patients undergoing coronary angiography, total and regional myocardial blood flow was determined by the 133Xe wash-out technique. A total of 39 pre- and 39 post-Valium injection wash-out curves were constructed. Results indicate (99% confidence) that intravenous Valium injection (0.1 mg/kg body weight) decreases myocardial blood flow 12--15%.", "PMID": 981643}
{"id": "pubmed23n0032_12683", "title": "Breast parenchymal patterns and their changes with age.", "content": "The appearance of the breast parenchyma was studied in 955 women to determine whether it changed with age. It was found that women initially classified as being at either a low risk or a very high risk of cancer do not demonstrate changes in the breast parenchyma pattern with age. However, breasts in young women which exhibit dysplastic changes on the initial examination (QDY) frequently do change, occasionally to a relatively lower-risk category but often to a normal or near-normal appearance. Such changes generally occur between the ages of 35 and 50.", "contents": "Breast parenchymal patterns and their changes with age. The appearance of the breast parenchyma was studied in 955 women to determine whether it changed with age. It was found that women initially classified as being at either a low risk or a very high risk of cancer do not demonstrate changes in the breast parenchyma pattern with age. However, breasts in young women which exhibit dysplastic changes on the initial examination (QDY) frequently do change, occasionally to a relatively lower-risk category but often to a normal or near-normal appearance. Such changes generally occur between the ages of 35 and 50.", "PMID": 981644}
{"id": "pubmed23n0032_12684", "title": "Thermographic patterns of the breast: a critical analysis of interpretation.", "content": "In a prospective evaluation, 1,000 consecutive breast thermograms were categorized as either normal or having 1 of 4 abnormal patterns: diffuse, asymmetrical, focal, or peri-areolar. Of 49 proved carcinomas, 43 produced an abnormal patterns; the asymmetric type was 3 times as common as the focal. Tumors producing focal patterns were slightly smaller than those producing asymmetrical patterns. Almost 30% of the non-malignant lesions produced an abnormal thermogram. This procedure can not generally be used to distinguish between benign and malignant lesions; there may be, therefore, little diagnostic value in categorizing abnormal thermograms. The usefulness of thermography in the detection of occult carcinomas has not been established.", "contents": "Thermographic patterns of the breast: a critical analysis of interpretation. In a prospective evaluation, 1,000 consecutive breast thermograms were categorized as either normal or having 1 of 4 abnormal patterns: diffuse, asymmetrical, focal, or peri-areolar. Of 49 proved carcinomas, 43 produced an abnormal patterns; the asymmetric type was 3 times as common as the focal. Tumors producing focal patterns were slightly smaller than those producing asymmetrical patterns. Almost 30% of the non-malignant lesions produced an abnormal thermogram. This procedure can not generally be used to distinguish between benign and malignant lesions; there may be, therefore, little diagnostic value in categorizing abnormal thermograms. The usefulness of thermography in the detection of occult carcinomas has not been established.", "PMID": 981645}
{"id": "pubmed23n0032_12685", "title": "Needle localization of nonpalpable breast lesions.", "content": "Mammographic identification of nonpalpable breast lesions suspicious for malignancy constitutes a valid indication for breast biopsy but localization is difficult. Percutaneous needle localization of such lesions, whether clustered microcalcifications, small masses, or focal areas of distorted mammary architecture, is an effective aid to the surgeon. The authors' experience in more than 80 cases is described and the necessity for specimen radiography is emphasized.", "contents": "Needle localization of nonpalpable breast lesions. Mammographic identification of nonpalpable breast lesions suspicious for malignancy constitutes a valid indication for breast biopsy but localization is difficult. Percutaneous needle localization of such lesions, whether clustered microcalcifications, small masses, or focal areas of distorted mammary architecture, is an effective aid to the surgeon. The authors' experience in more than 80 cases is described and the necessity for specimen radiography is emphasized.", "PMID": 981646}
{"id": "pubmed23n0032_12686", "title": "Osteochondritis dissecans complicating Legg-Perthes disease. A report of four cases.", "content": "Osteochondritis dissecans of the femoral head occurs as a rare complication in the late onset of Legg-Perthes disease. The etiology of this complication is uncertain. Theories include: (a) persistence of an ununited fragment and (b) fragmentation of the femoral head weakened by revascularization accompanying healing. The area of osteochondritis dissecans may not be readily apparent on routine radiographs and a high index of suspicion is necessary for diagnosis. Four cases of osteochondritis dissecans of the hip presenting 6--18 years after the diagnosis of Legg-Perthes disease are described. Three patients were studied by arthrography, and the findings correlated well with symptomatology.", "contents": "Osteochondritis dissecans complicating Legg-Perthes disease. A report of four cases. Osteochondritis dissecans of the femoral head occurs as a rare complication in the late onset of Legg-Perthes disease. The etiology of this complication is uncertain. Theories include: (a) persistence of an ununited fragment and (b) fragmentation of the femoral head weakened by revascularization accompanying healing. The area of osteochondritis dissecans may not be readily apparent on routine radiographs and a high index of suspicion is necessary for diagnosis. Four cases of osteochondritis dissecans of the hip presenting 6--18 years after the diagnosis of Legg-Perthes disease are described. Three patients were studied by arthrography, and the findings correlated well with symptomatology.", "PMID": 981647}
{"id": "pubmed23n0032_12687", "title": "Unilateral arch hypertrophy with spinous process tilt: a sign of arch deficiency.", "content": "Unilateral arch hypertrophy (UAH) may be generated by a contralateral deficiency which is most commonly a pars defect (43 out of 50 patients studied). Such a defect can also cause rotational instability. When present, both can be easily detected on simple anteroposterior views through the resulting pedicular and pars asymmetry and spinous process tilt. From simple anteroposterior views, one can predict that at least one arch defect does exist, that it is unstable, and not of recent origin.", "contents": "Unilateral arch hypertrophy with spinous process tilt: a sign of arch deficiency. Unilateral arch hypertrophy (UAH) may be generated by a contralateral deficiency which is most commonly a pars defect (43 out of 50 patients studied). Such a defect can also cause rotational instability. When present, both can be easily detected on simple anteroposterior views through the resulting pedicular and pars asymmetry and spinous process tilt. From simple anteroposterior views, one can predict that at least one arch defect does exist, that it is unstable, and not of recent origin.", "PMID": 981648}
{"id": "pubmed23n0032_12688", "title": "Normal \"Cupid's bow\" contour of the lower lumbar vertebrae.", "content": "The inferior end plates of the 3d, 4th, and 5th lumbar vertebral bodies frequently have paired parasagittal concavities when viewed in the frontal projection. When viewed in the lateral projection, the concavities superimpose, lying in the posterior portion of the vertebral body. This normal contour of the end plate should not be confused with other vertebral body anomalies having clinical importance. The incidence and degree of end plate depression are discussed.", "contents": "Normal \"Cupid's bow\" contour of the lower lumbar vertebrae. The inferior end plates of the 3d, 4th, and 5th lumbar vertebral bodies frequently have paired parasagittal concavities when viewed in the frontal projection. When viewed in the lateral projection, the concavities superimpose, lying in the posterior portion of the vertebral body. This normal contour of the end plate should not be confused with other vertebral body anomalies having clinical importance. The incidence and degree of end plate depression are discussed.", "PMID": 981649}
{"id": "pubmed23n0032_12689", "title": "Hemodynamic changes after distal splenorenal shunt studied by sequential angiography.", "content": "Distal splenorenal shunts were made in 25 patients with cirrhosis of the liver and portal hypertension. Angiograms were obtained in 16 patients at one week, three and six months, and at one year postoperatively. Portal vein flow had diminished in all patients compared with flow seen in preoperative angiograms. Hepatofugal flow developed in 6 patients during the follow-up period, and in 2 patients only one week postoperatively. The shunt remained patent in all of the patients but one.", "contents": "Hemodynamic changes after distal splenorenal shunt studied by sequential angiography. Distal splenorenal shunts were made in 25 patients with cirrhosis of the liver and portal hypertension. Angiograms were obtained in 16 patients at one week, three and six months, and at one year postoperatively. Portal vein flow had diminished in all patients compared with flow seen in preoperative angiograms. Hepatofugal flow developed in 6 patients during the follow-up period, and in 2 patients only one week postoperatively. The shunt remained patent in all of the patients but one.", "PMID": 981650}
{"id": "pubmed23n0032_12690", "title": "Stratification in the gallbladder on intravenous cholangiography. The value of delayed or 24-hour radiographs.", "content": "Errors in interpretation of intravenous cholangiograms are often made if 24-hour radiographs are not obtained. Three cases are presented in which radiographs, obtained 1/2--4 hours after administration of contrast material, failed to demonstrate gallstones in the visualized gallbladder. However, 24-hour radiographs revealed calculi in all three patients. Stratification of bile is thought to be the mechanism responsible. Delayed radiographs assure proper mixing of contrast material and bile, and provide optimal visualization of the gallbladder in patients undergoing intravenous cholangiography.", "contents": "Stratification in the gallbladder on intravenous cholangiography. The value of delayed or 24-hour radiographs. Errors in interpretation of intravenous cholangiograms are often made if 24-hour radiographs are not obtained. Three cases are presented in which radiographs, obtained 1/2--4 hours after administration of contrast material, failed to demonstrate gallstones in the visualized gallbladder. However, 24-hour radiographs revealed calculi in all three patients. Stratification of bile is thought to be the mechanism responsible. Delayed radiographs assure proper mixing of contrast material and bile, and provide optimal visualization of the gallbladder in patients undergoing intravenous cholangiography.", "PMID": 981651}
{"id": "pubmed23n0032_12691", "title": "The normal and abnormal development of the appendix. A radiographic assessment.", "content": "Radiographic examinations of the colon in the adult demonstrate a wide variety of contours of the interior cecal segment and appendix. These findings are a result of the normal developmental process of the appendix which can be arbitrarily separated into four stages. Examples of appendiceal development arrested at different stages, as well as variations of the normal adult appendix, are explained and illustrated. Forms of primitive development and lack of development (agenesis) are discussed.", "contents": "The normal and abnormal development of the appendix. A radiographic assessment. Radiographic examinations of the colon in the adult demonstrate a wide variety of contours of the interior cecal segment and appendix. These findings are a result of the normal developmental process of the appendix which can be arbitrarily separated into four stages. Examples of appendiceal development arrested at different stages, as well as variations of the normal adult appendix, are explained and illustrated. Forms of primitive development and lack of development (agenesis) are discussed.", "PMID": 981652}
{"id": "pubmed23n0032_12692", "title": "Renal abnormalities in agnogenic myeloid metaplasia.", "content": "Abnormal excretory urograms of 5 patients with agnogenic myeloid metaplasia and extramedullary hematopoiesis are presented, with radiological/pathological correlation where possible. Findings include renal enlargement with distortion of calyces and renal peripelvic masses.", "contents": "Renal abnormalities in agnogenic myeloid metaplasia. Abnormal excretory urograms of 5 patients with agnogenic myeloid metaplasia and extramedullary hematopoiesis are presented, with radiological/pathological correlation where possible. Findings include renal enlargement with distortion of calyces and renal peripelvic masses.", "PMID": 981653}
{"id": "pubmed23n0032_12693", "title": "Unilateral absence of excretion at urography after abdominal trauma.", "content": "The unilateral absence of excretion at urography is particularly significant in the context of prior abdominal trauma. Of 23 such cases studied, 17 involved traumatic main renal artery occlusion; only 1 of these 17 kidneys was salvaged. Since earlier diagnosis may improve surgical results, urography should be abbreviated in favor of renal angiography, to differentiate renal artery occlusion from other renal injuries, agenesis, and preexisting renal disease. Clinical and plain radiographic features provide few clues. Renal venography, ultrasonography, and retrograde ureteropyelography should be used only as secondary procedures. The role of renal scintiangiography remains to be determined.", "contents": "Unilateral absence of excretion at urography after abdominal trauma. The unilateral absence of excretion at urography is particularly significant in the context of prior abdominal trauma. Of 23 such cases studied, 17 involved traumatic main renal artery occlusion; only 1 of these 17 kidneys was salvaged. Since earlier diagnosis may improve surgical results, urography should be abbreviated in favor of renal angiography, to differentiate renal artery occlusion from other renal injuries, agenesis, and preexisting renal disease. Clinical and plain radiographic features provide few clues. Renal venography, ultrasonography, and retrograde ureteropyelography should be used only as secondary procedures. The role of renal scintiangiography remains to be determined.", "PMID": 981654}
{"id": "pubmed23n0032_12694", "title": "Enzymatic evidence of renal tubular damage following renal angiography.", "content": "Ligandin, a glutathione (GSH) transferase, is a protein found in proximal tubular cells as well as in other tissues. It does not occur in the urine of normal subjects; however, its presence has been detected in the urine of some patients who have undergone renal angiography. This finding suggests renal tubular cell damage due to the procedure.", "contents": "Enzymatic evidence of renal tubular damage following renal angiography. Ligandin, a glutathione (GSH) transferase, is a protein found in proximal tubular cells as well as in other tissues. It does not occur in the urine of normal subjects; however, its presence has been detected in the urine of some patients who have undergone renal angiography. This finding suggests renal tubular cell damage due to the procedure.", "PMID": 981655}
{"id": "pubmed23n0032_12695", "title": "The internal mammary artery: an overlooked collateral pathway to the leg.", "content": "In 10 patients with an occluded aorta, angiography performed with the catheter in the abdominal aorta failed to opacify the reconstituted distal extremity arteries. Repeat injection into the thoracic aorta resulted in adequate opacification of the leg vessels through internal mammary-inferior epigastric artery collaterals. Moving the catheter from the abdominal aorta to the ascending aorta or selectively into the internal mammary artery is suggested when standard abdominal aortic injection fails to demonstrate runoff vessels.", "contents": "The internal mammary artery: an overlooked collateral pathway to the leg. In 10 patients with an occluded aorta, angiography performed with the catheter in the abdominal aorta failed to opacify the reconstituted distal extremity arteries. Repeat injection into the thoracic aorta resulted in adequate opacification of the leg vessels through internal mammary-inferior epigastric artery collaterals. Moving the catheter from the abdominal aorta to the ascending aorta or selectively into the internal mammary artery is suggested when standard abdominal aortic injection fails to demonstrate runoff vessels.", "PMID": 981656}
{"id": "pubmed23n0032_12696", "title": "Arteriography of peripheral hemangiomas.", "content": "Arteriography has proved useful in evaluation of 17 patients with peripheral soft-tissue hemangiomas (15 benign, 2 malignant) by defining their extent, degree of vascularity, and source of vascular supply and allowing differentiation from arteriovenous malformations. In 11 cases (all benign), moderate fine-caliber hypervascularity with some degree of staining was seen. In 6 (4 benign, 2 malignant) there was marked hypervascularity with coarse, irregular vessels. The coarse or fine hypervascularity which characterizes most benign hemangiomas closely resembles that seen in malignant soft-tissue tumors and may preclude correct diagnosis by angiography alone. However, peripheral hemangiomas frequently exhibit distinctive clinical features which when combined with the arteriographic findings enable the radiologist to identify the lesion.", "contents": "Arteriography of peripheral hemangiomas. Arteriography has proved useful in evaluation of 17 patients with peripheral soft-tissue hemangiomas (15 benign, 2 malignant) by defining their extent, degree of vascularity, and source of vascular supply and allowing differentiation from arteriovenous malformations. In 11 cases (all benign), moderate fine-caliber hypervascularity with some degree of staining was seen. In 6 (4 benign, 2 malignant) there was marked hypervascularity with coarse, irregular vessels. The coarse or fine hypervascularity which characterizes most benign hemangiomas closely resembles that seen in malignant soft-tissue tumors and may preclude correct diagnosis by angiography alone. However, peripheral hemangiomas frequently exhibit distinctive clinical features which when combined with the arteriographic findings enable the radiologist to identify the lesion.", "PMID": 981657}
{"id": "pubmed23n0032_12697", "title": "Lymphography in clinically suspected lymphoma: a retrospective analysis of 63 cases.", "content": "Pedal lymphography was performed for 63 patients with clinical findings (chiefly unexplained pyrexia or splenomegaly) suggestive of lymphoma involving the retroperitoneal nodes. The lymphogram was abnormal in 17 cases. Lymphoma was found in 5, metastatic carcinoma in 3, malignant histiocytes in 1, and benign hyperplasia or fibrolipomatous nodal changes in 4 (histiological tests were not obtained in the other 4). An abnormal lymphogram of the retroperitoneal nodes constitutes an indication for laparotomy; the lymphographic appearance of lymphoma is nonspecific, and histologic confirmation should be sought.", "contents": "Lymphography in clinically suspected lymphoma: a retrospective analysis of 63 cases. Pedal lymphography was performed for 63 patients with clinical findings (chiefly unexplained pyrexia or splenomegaly) suggestive of lymphoma involving the retroperitoneal nodes. The lymphogram was abnormal in 17 cases. Lymphoma was found in 5, metastatic carcinoma in 3, malignant histiocytes in 1, and benign hyperplasia or fibrolipomatous nodal changes in 4 (histiological tests were not obtained in the other 4). An abnormal lymphogram of the retroperitoneal nodes constitutes an indication for laparotomy; the lymphographic appearance of lymphoma is nonspecific, and histologic confirmation should be sought.", "PMID": 981658}
{"id": "pubmed23n0032_12698", "title": "Attenuation measurements of whole blood and blood fractions in computed tomography.", "content": "Computed tomography (CT) provides an extraordinarily effective means of diagnosing intracranial hemorrhages. Normal blood has a relatively high x-ray attenuation in the circulation and following extravasation, permitting the discrimination by CT of many pathologically enlarged vascular spaces containing circulating blood and/or blood clot (such as arteriovenous malformations and large aneurysms) in addition to recent hemorrhages, from continguous anatomical structures. To promote further understanding of the factors contributing to the attenuation of blood in normal and abnormal states. the attenuations of various preparations of whole blood and blood fractions were investigated in further detail.", "contents": "Attenuation measurements of whole blood and blood fractions in computed tomography. Computed tomography (CT) provides an extraordinarily effective means of diagnosing intracranial hemorrhages. Normal blood has a relatively high x-ray attenuation in the circulation and following extravasation, permitting the discrimination by CT of many pathologically enlarged vascular spaces containing circulating blood and/or blood clot (such as arteriovenous malformations and large aneurysms) in addition to recent hemorrhages, from continguous anatomical structures. To promote further understanding of the factors contributing to the attenuation of blood in normal and abnormal states. the attenuations of various preparations of whole blood and blood fractions were investigated in further detail.", "PMID": 981659}
{"id": "pubmed23n0032_12699", "title": "Computed tomography in cerebral abscess.", "content": "Computed tomography (CT) scans of 9 surgically proved cases of cerebral abscess were reviewed. The CT characteristics were studied, as were the postoperative scans. Also assessed were the contributions to the diagnosis of the clinical features, plain radiographic examinations, radionuclide imaging, and angiography. The accuracy and information content of CT scans of cerebral abscesses should lead to a significant reduction in morbidity and mortality.", "contents": "Computed tomography in cerebral abscess. Computed tomography (CT) scans of 9 surgically proved cases of cerebral abscess were reviewed. The CT characteristics were studied, as were the postoperative scans. Also assessed were the contributions to the diagnosis of the clinical features, plain radiographic examinations, radionuclide imaging, and angiography. The accuracy and information content of CT scans of cerebral abscesses should lead to a significant reduction in morbidity and mortality.", "PMID": 981660}
{"id": "pubmed23n0032_12700", "title": "Persistent trigminal artery in the fetus.", "content": "Radiological and anatomical studies of a persistent trigeminal artery in three human fetuses are reported. In one fetus there was also an anastomotic artery connecting one of the carotid arteries to the contralateral inferior cerebellar artery. In all three cases, the trigeminal arteries gave branches to the pons. In addition to the pontine branches, one trigeminal artery gave an anastomotic branch to the posterior inferior cerebellar artery and the other trigeminal artery gave branches to both the superior cerebellar artery and the internal auditory artery.", "contents": "Persistent trigminal artery in the fetus. Radiological and anatomical studies of a persistent trigeminal artery in three human fetuses are reported. In one fetus there was also an anastomotic artery connecting one of the carotid arteries to the contralateral inferior cerebellar artery. In all three cases, the trigeminal arteries gave branches to the pons. In addition to the pontine branches, one trigeminal artery gave an anastomotic branch to the posterior inferior cerebellar artery and the other trigeminal artery gave branches to both the superior cerebellar artery and the internal auditory artery.", "PMID": 981661}
{"id": "pubmed23n0032_12701", "title": "Technetium-99m pyridoxylideneglutamate (P.G.) cholescintigraphy.", "content": "Technetium-99m P.G. cholescintigraphy was performed in 27 human volunteers and 81 patients referred for hepatobiliary tract disease. The gallbladder, biliary system, and gastrointestinal tract were well visualized in the normal patients and volunteers. The gallbladder was not visualized in 22 patients with histologically proved cholecystitis with cystic duct obstruction. Nine patients with complet extrahepatic obstruction of the common bile duct were correctly diagnosed. Hepatocellular disease and incomplete obstruction, with and without jaundice, were diagnosed with this technique. Oral cholecystography is superior to this method for the detection of cholelithiasis in nonjaundiced patients.", "contents": "Technetium-99m pyridoxylideneglutamate (P.G.) cholescintigraphy. Technetium-99m P.G. cholescintigraphy was performed in 27 human volunteers and 81 patients referred for hepatobiliary tract disease. The gallbladder, biliary system, and gastrointestinal tract were well visualized in the normal patients and volunteers. The gallbladder was not visualized in 22 patients with histologically proved cholecystitis with cystic duct obstruction. Nine patients with complet extrahepatic obstruction of the common bile duct were correctly diagnosed. Hepatocellular disease and incomplete obstruction, with and without jaundice, were diagnosed with this technique. Oral cholecystography is superior to this method for the detection of cholelithiasis in nonjaundiced patients.", "PMID": 981662}
{"id": "pubmed23n0032_12702", "title": "Solitary abnormalities in bone scans of patients with extraosseous malignancies.", "content": "The incidence and significance of solitary bone scan abnormalities were assessed in a study of 1,129 consecutive patients with extraskeletal primary malignancies. Solitary abnormalities were encountered in 172 cases (15%). The etiology of the scan abnormality was established in 90 of the 172 cases; 58 (64%) were due to metastatic disease, and 32 (36%) were secondary to a benign process. A significant percentage of solitary scan abnormalities is due to benign disease processes, even in patients with proved extraosseous malignancies.", "contents": "Solitary abnormalities in bone scans of patients with extraosseous malignancies. The incidence and significance of solitary bone scan abnormalities were assessed in a study of 1,129 consecutive patients with extraskeletal primary malignancies. Solitary abnormalities were encountered in 172 cases (15%). The etiology of the scan abnormality was established in 90 of the 172 cases; 58 (64%) were due to metastatic disease, and 32 (36%) were secondary to a benign process. A significant percentage of solitary scan abnormalities is due to benign disease processes, even in patients with proved extraosseous malignancies.", "PMID": 981663}
{"id": "pubmed23n0032_12703", "title": "Unilateral breast uptake of 67Ga from breast feeding.", "content": "Unilateral 67Ga uptake was observed in the left breast of a 23-year-old woman who had nursed her infant on that side for 10 months. Careful clinical examination and follow-up failed to reveal any evidence of breast disease. Unilateral breast feeding should be considered in the differential diagnosis of asymmetric 67Ga localization in the breast.", "contents": "Unilateral breast uptake of 67Ga from breast feeding. Unilateral 67Ga uptake was observed in the left breast of a 23-year-old woman who had nursed her infant on that side for 10 months. Careful clinical examination and follow-up failed to reveal any evidence of breast disease. Unilateral breast feeding should be considered in the differential diagnosis of asymmetric 67Ga localization in the breast.", "PMID": 981664}
{"id": "pubmed23n0032_12704", "title": "The usefulness of the posterior oblique views in perfusion lung imaging.", "content": "A total of 116 perfusion lung images, with and without posterior oblique views, were compared by four trained observers. There was a significant increase in the number of perfusion defects detected when the posterior oblique views were included. Pairs of projections of 89 lung images were read by the four observers. The percentage of correct readings for the posterior oblique views was 78% compared with 63% and 56% for the lateral views and AP views, respectively. Inclusion of the posterior oblique views as a routine part of the examination is recommended.", "contents": "The usefulness of the posterior oblique views in perfusion lung imaging. A total of 116 perfusion lung images, with and without posterior oblique views, were compared by four trained observers. There was a significant increase in the number of perfusion defects detected when the posterior oblique views were included. Pairs of projections of 89 lung images were read by the four observers. The percentage of correct readings for the posterior oblique views was 78% compared with 63% and 56% for the lateral views and AP views, respectively. Inclusion of the posterior oblique views as a routine part of the examination is recommended.", "PMID": 981665}
{"id": "pubmed23n0032_12705", "title": "Maxillary-facial abnormalities assessed by bone imaging.", "content": "Eighty-eight patients with maxillary-facial abnormalities were studied with 99mTc-labeled phosphate compounds. Straight and tilted anterior views plus oblique orbital and lateral views were correlated with 201 thallium transmission images of a dried skull. Interpretation of any single view may be hampered by various superimposed structures. Careful patient positioning with multiple views allows for parallactic correlation which is crucial if radiotherapy, biopsy, or corroborative studies are planned.", "contents": "Maxillary-facial abnormalities assessed by bone imaging. Eighty-eight patients with maxillary-facial abnormalities were studied with 99mTc-labeled phosphate compounds. Straight and tilted anterior views plus oblique orbital and lateral views were correlated with 201 thallium transmission images of a dried skull. Interpretation of any single view may be hampered by various superimposed structures. Careful patient positioning with multiple views allows for parallactic correlation which is crucial if radiotherapy, biopsy, or corroborative studies are planned.", "PMID": 981666}
{"id": "pubmed23n0032_12706", "title": "Early detection of stress fractures using 99mTc-polyphosphate.", "content": "Clinical experience with 99mTc-polyphosphate scintigraphy in more than 200 patients with suspected stress fractures is presented. Stress fractures occur in bone which has been weakened as a result of accelerated physiological change induced by excessive muscular and mechanical activity. Unlike traumatic fractures, they are not accompanied by radiographic abnormality early in their course. Bone scintigraphy is sensitive enough to detect the physiological alterations found in stress fractures and was responsible for decreased morbidity in this series of patients due to early diagnosis.", "contents": "Early detection of stress fractures using 99mTc-polyphosphate. Clinical experience with 99mTc-polyphosphate scintigraphy in more than 200 patients with suspected stress fractures is presented. Stress fractures occur in bone which has been weakened as a result of accelerated physiological change induced by excessive muscular and mechanical activity. Unlike traumatic fractures, they are not accompanied by radiographic abnormality early in their course. Bone scintigraphy is sensitive enough to detect the physiological alterations found in stress fractures and was responsible for decreased morbidity in this series of patients due to early diagnosis.", "PMID": 981667}
{"id": "pubmed23n0032_12707", "title": "Focal splenic defects.", "content": "Review of more than 2,000 liver-spleen scans revealed 18 cases of focal splenic defects. Three major categories are defined: lymphoma, infarctions, and metastatic tumor. Clinical presentation is important in determining the precise etiology, and a differential diagnosis is presented. It appears that: (a) reticulum cell carcinoma is as likely to cause defects as is Hodgkin's disease; (b) splenic infarction is related to pancreatic disease through the splenic vein; and (c) malignant melanoma is the most common secondary deposit as detected by scanning.", "contents": "Focal splenic defects. Review of more than 2,000 liver-spleen scans revealed 18 cases of focal splenic defects. Three major categories are defined: lymphoma, infarctions, and metastatic tumor. Clinical presentation is important in determining the precise etiology, and a differential diagnosis is presented. It appears that: (a) reticulum cell carcinoma is as likely to cause defects as is Hodgkin's disease; (b) splenic infarction is related to pancreatic disease through the splenic vein; and (c) malignant melanoma is the most common secondary deposit as detected by scanning.", "PMID": 981668}
{"id": "pubmed23n0032_12708", "title": "Possible adverse effect of methylglucamine diatrizoate compounds on the bowel of newborn infants with meconium ileus.", "content": "Gastrografin (methylglucamine diatrizoate) enemas were carried out in 2 newborn infants with meconium ileus. Evacuation was slow and incomplete. Both patients died within 72 hours following enemas from bowel necrosis, perforation and peritonitis. Although it is not possible to implicate Gastrografin directly as the cause, it is suggested that it may have contributed substantially to bowel necrosis. Recent experimental evidence of colonic inflammation and occasionally necrosis caused by Gastrografin lends support to this hypothesis. Caution should be exercised to prevent not only the systemic osmotic effects of Gastrografin, but also potential local injury to the bowel, especially when underlying disease interferes with intestinal viability.", "contents": "Possible adverse effect of methylglucamine diatrizoate compounds on the bowel of newborn infants with meconium ileus. Gastrografin (methylglucamine diatrizoate) enemas were carried out in 2 newborn infants with meconium ileus. Evacuation was slow and incomplete. Both patients died within 72 hours following enemas from bowel necrosis, perforation and peritonitis. Although it is not possible to implicate Gastrografin directly as the cause, it is suggested that it may have contributed substantially to bowel necrosis. Recent experimental evidence of colonic inflammation and occasionally necrosis caused by Gastrografin lends support to this hypothesis. Caution should be exercised to prevent not only the systemic osmotic effects of Gastrografin, but also potential local injury to the bowel, especially when underlying disease interferes with intestinal viability.", "PMID": 981669}
{"id": "pubmed23n0032_12709", "title": "Intestinal perforation. An infrequent complication of ventriculo-peritoneal shunts.", "content": "Bowel perforation by ventriculo-peritoneal shunts occurred in two children with no abdominal symptoms. The diagnosis in each instance was confirmed by opacification of the colon in one child and the small bowel in the other via contrast medium injection of the distal shunt tubing. \"Shuntograms\" are recommended for all patients with no readily evident cause of dysfunctioning cerebrospinal fluid-peritoneal shunts.", "contents": "Intestinal perforation. An infrequent complication of ventriculo-peritoneal shunts. Bowel perforation by ventriculo-peritoneal shunts occurred in two children with no abdominal symptoms. The diagnosis in each instance was confirmed by opacification of the colon in one child and the small bowel in the other via contrast medium injection of the distal shunt tubing. \"Shuntograms\" are recommended for all patients with no readily evident cause of dysfunctioning cerebrospinal fluid-peritoneal shunts.", "PMID": 981670}
{"id": "pubmed23n0032_12710", "title": "Delayed opacification in congenital multicystic dysplastic kidney, an important Roentgen sign.", "content": "Two cases of delayed opacification of multiple irregular cystic spaces are reported in unilateral congenital multicystic kidney. This finding should be added to the basic excretory urographic sings of poor function, cystic spaces on total body opacification, and calyceal crescents, and indicates the need for delayed films in all such cases. Reevaluation of the necessity for further diagnostic study and nephrectomy are discussed.", "contents": "Delayed opacification in congenital multicystic dysplastic kidney, an important Roentgen sign. Two cases of delayed opacification of multiple irregular cystic spaces are reported in unilateral congenital multicystic kidney. This finding should be added to the basic excretory urographic sings of poor function, cystic spaces on total body opacification, and calyceal crescents, and indicates the need for delayed films in all such cases. Reevaluation of the necessity for further diagnostic study and nephrectomy are discussed.", "PMID": 981671}
{"id": "pubmed23n0032_12711", "title": "Ultrasonic diagnosis of a popliteal artery aneurysm.", "content": "Ultrasound techniques are very effective in screening painful masses of the popliteal space. These techniques easily differentiate popliteal cysts from thrombophlebitis, and in addition permit sequential evaluation without patient discomfort or invasion. We report its use in diagnosing a popliteal artery aneurysm, another cause of a painful popliteal mass.", "contents": "Ultrasonic diagnosis of a popliteal artery aneurysm. Ultrasound techniques are very effective in screening painful masses of the popliteal space. These techniques easily differentiate popliteal cysts from thrombophlebitis, and in addition permit sequential evaluation without patient discomfort or invasion. We report its use in diagnosing a popliteal artery aneurysm, another cause of a painful popliteal mass.", "PMID": 981672}
{"id": "pubmed23n0032_12712", "title": "Prospective studies with the CRE formula of prolonged fractionation schedules.", "content": "Different dose schedules prospectively calculated with the Cumulative Radiation Effect (CRE) formula were used for irradiation of inoperable breast carcinomas. Fractionation with 2 X 260 rad and 2 X 450 rad twice a week, and total doses corresponding to CRE levels 1900 to 2300, were compared. The results showed that prolonged fractionation increased the tolerance of normal skin and the therapeutic ration with respect to early radiation reactions. Late reactions could not be assessed as yet because their development requires several years. With the prolonged course of treatment and 2 X 260 rad per week, no moist desquamation developed despite dose levels between 6800 and 9400 rad. Skin reactions were milder than those in the rapidly treated areas, indicating that the CRE formula cannot exactly predict acute skin reactions for very prolonged treatment schedules. The tumor regressed continuously during the irradiation schedule.", "contents": "Prospective studies with the CRE formula of prolonged fractionation schedules. Different dose schedules prospectively calculated with the Cumulative Radiation Effect (CRE) formula were used for irradiation of inoperable breast carcinomas. Fractionation with 2 X 260 rad and 2 X 450 rad twice a week, and total doses corresponding to CRE levels 1900 to 2300, were compared. The results showed that prolonged fractionation increased the tolerance of normal skin and the therapeutic ration with respect to early radiation reactions. Late reactions could not be assessed as yet because their development requires several years. With the prolonged course of treatment and 2 X 260 rad per week, no moist desquamation developed despite dose levels between 6800 and 9400 rad. Skin reactions were milder than those in the rapidly treated areas, indicating that the CRE formula cannot exactly predict acute skin reactions for very prolonged treatment schedules. The tumor regressed continuously during the irradiation schedule.", "PMID": 981673}
{"id": "pubmed23n0032_12713", "title": "Age as a prognostic indicator in carcinoma of the lung.", "content": "Selected elderly patients with squamous cell carcinoma localized to the lung should be considered for radical irradiation. Age should not be used as a negative prognostic indicator. This group of patients has a lower incidence of distant metastasis and local control may produce long-term survival. Radical irradiation is well tolerated, particularly as a split course, and serious complications are few.", "contents": "Age as a prognostic indicator in carcinoma of the lung. Selected elderly patients with squamous cell carcinoma localized to the lung should be considered for radical irradiation. Age should not be used as a negative prognostic indicator. This group of patients has a lower incidence of distant metastasis and local control may produce long-term survival. Radical irradiation is well tolerated, particularly as a split course, and serious complications are few.", "PMID": 981674}
{"id": "pubmed23n0032_12714", "title": "Repair of potentially lethan radiation damage: comparison of neutron and X-ray RBE and implications for radiation therapy.", "content": "Experiments with Chinese hamster cells have shown that neutron irradiation does not result in repair of potentially lethal damage (PLD), i.e., that which can be influenced by changes in environmental conditions following irradiation. Since PLD is presumed to be repaired in tumors but not in normal tissues, this absence of differential sparing of tumor cells relative to normal tissues--a feature characteristic of irradiation with x rays--represents an advantage of neutrons in addition to their reduced oxygen effect. At a given dose, the difference in relative biological effectiveness (RBE) between tumors and normal tissues corresponds to a 5% increase in tumor dose with no concomitant increase in dose to normal tissues, which could be significant in cancer therapy.", "contents": "Repair of potentially lethan radiation damage: comparison of neutron and X-ray RBE and implications for radiation therapy. Experiments with Chinese hamster cells have shown that neutron irradiation does not result in repair of potentially lethal damage (PLD), i.e., that which can be influenced by changes in environmental conditions following irradiation. Since PLD is presumed to be repaired in tumors but not in normal tissues, this absence of differential sparing of tumor cells relative to normal tissues--a feature characteristic of irradiation with x rays--represents an advantage of neutrons in addition to their reduced oxygen effect. At a given dose, the difference in relative biological effectiveness (RBE) between tumors and normal tissues corresponds to a 5% increase in tumor dose with no concomitant increase in dose to normal tissues, which could be significant in cancer therapy.", "PMID": 981675}
{"id": "pubmed23n0032_12715", "title": "Beta-aminopropionitrile as a radiation reaction preventive agent.", "content": "Beta-aminopropionitrile (BAPN), an inhibitor of collagen maturation, was tested as a radiation reaction preventive agent (RRPA) using radiation-induced lung fibrosis in rats and mice as a model. Following pulmonary irradiation, treatment with BAPN significantly prevented an increase in collagen content as measured by hydroxyproline analysis. This effect persisted during BAPN maintenance for periods of up to 10 weeks but was lost when the drug was discontinued. BAPN administration did not increase the mouse lung LD50/160 in the drug doses employed in this study. Other possible RRPAs deserving further study include D-penicillamine and beclomethasone dipropionate.", "contents": "Beta-aminopropionitrile as a radiation reaction preventive agent. Beta-aminopropionitrile (BAPN), an inhibitor of collagen maturation, was tested as a radiation reaction preventive agent (RRPA) using radiation-induced lung fibrosis in rats and mice as a model. Following pulmonary irradiation, treatment with BAPN significantly prevented an increase in collagen content as measured by hydroxyproline analysis. This effect persisted during BAPN maintenance for periods of up to 10 weeks but was lost when the drug was discontinued. BAPN administration did not increase the mouse lung LD50/160 in the drug doses employed in this study. Other possible RRPAs deserving further study include D-penicillamine and beclomethasone dipropionate.", "PMID": 981676}
{"id": "pubmed23n0032_12716", "title": "Single monitor stereoradiological television system using PLZT electrooptic shutters.", "content": "A new family of materials consisting of lead lanthanium zirconate titanate (PLZT) has evolved from recent advances in ferroelectric ceramics. PLZT electrooptic ceramics may be used as electronically triggered high-contrast optical shutters in lightweight stereoscopic viewing devices. A PLZT electrooptic shutter stereoradiology system in which dual x-ray tubes are used to generate stereo pairs of fluoroscopic images is presented. This system requires only a single television monitor for stereoscopic viewing by one or more observers. The viewer which contains the optical shutters is compact, light-weight, and can be attached easily to normal eyeglasses.", "contents": "Single monitor stereoradiological television system using PLZT electrooptic shutters. A new family of materials consisting of lead lanthanium zirconate titanate (PLZT) has evolved from recent advances in ferroelectric ceramics. PLZT electrooptic ceramics may be used as electronically triggered high-contrast optical shutters in lightweight stereoscopic viewing devices. A PLZT electrooptic shutter stereoradiology system in which dual x-ray tubes are used to generate stereo pairs of fluoroscopic images is presented. This system requires only a single television monitor for stereoscopic viewing by one or more observers. The viewer which contains the optical shutters is compact, light-weight, and can be attached easily to normal eyeglasses.", "PMID": 981678}
{"id": "pubmed23n0032_12717", "title": "An aid in sialographic studies. A newly devised curved sialographic obturator cannula.", "content": "A newly devised curved side-hole cannula for use in sialographic procedures is presented. A tapered metallic cuff serves as a retention plug to prevent backflow and escape of the contrast medium.", "contents": "An aid in sialographic studies. A newly devised curved sialographic obturator cannula. A newly devised curved side-hole cannula for use in sialographic procedures is presented. A tapered metallic cuff serves as a retention plug to prevent backflow and escape of the contrast medium.", "PMID": 981681}
{"id": "pubmed23n0032_12718", "title": "Radionuclide venography of the lower extremities and inferior vena cava by continuous injection and moving bed gamma camera technique.", "content": "Radionuclide venography has proved to be a tedious procedure because of the need for multiple imaging set-ups with coordinated, repeated injections of radionuclide. The use of a moving bed gamma camera has shortened the procedure. This method enables the entire sapheno-femoral-iliac-caval system to be visualized in continuity on a single film. The moving table top system also adequately imaged the suspected areas of thrombi or endothelial damage on the post-exercise study on a single film.", "contents": "Radionuclide venography of the lower extremities and inferior vena cava by continuous injection and moving bed gamma camera technique. Radionuclide venography has proved to be a tedious procedure because of the need for multiple imaging set-ups with coordinated, repeated injections of radionuclide. The use of a moving bed gamma camera has shortened the procedure. This method enables the entire sapheno-femoral-iliac-caval system to be visualized in continuity on a single film. The moving table top system also adequately imaged the suspected areas of thrombi or endothelial damage on the post-exercise study on a single film.", "PMID": 981682}
{"id": "pubmed23n0032_12719", "title": "Selective incorporation of 14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to 14C-prostaglandins.", "content": "A method is described for the efficient incorporation of radioactive arachidonic acid into the lipids of rabbit hearts and kidneys. Infusion of 14C-arachidonate through perfused tissues resulted in the quantitative removel of label from the media. Analysis of the lipids from tissues labeled by this procedure revealed that the majority of the 14C-arachidonate was incorporated into phospholipids. Essentially all of the radioactivity in phosphatidylcholine was found in the 2-position. Subsequent to the 14C-arachidonate infusion, stimulation of prostaglandin biosynthesis (e.g. by bradykinin) resulted in the release of radioactive prostaglandins. This suggests that the 14C-arachidonate is incorporated in a manner such that it is available for hormone-stimulated prostaglandin biosynthesis. The method described allows both qualitative and quantitative analysis of arachidonate metabolism in intact tissues and offers significant advantages over other presently used methods.", "contents": "Selective incorporation of 14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to 14C-prostaglandins. A method is described for the efficient incorporation of radioactive arachidonic acid into the lipids of rabbit hearts and kidneys. Infusion of 14C-arachidonate through perfused tissues resulted in the quantitative removel of label from the media. Analysis of the lipids from tissues labeled by this procedure revealed that the majority of the 14C-arachidonate was incorporated into phospholipids. Essentially all of the radioactivity in phosphatidylcholine was found in the 2-position. Subsequent to the 14C-arachidonate infusion, stimulation of prostaglandin biosynthesis (e.g. by bradykinin) resulted in the release of radioactive prostaglandins. This suggests that the 14C-arachidonate is incorporated in a manner such that it is available for hormone-stimulated prostaglandin biosynthesis. The method described allows both qualitative and quantitative analysis of arachidonate metabolism in intact tissues and offers significant advantages over other presently used methods.", "PMID": 981699}
{"id": "pubmed23n0032_12720", "title": "Polymorphonuclear leukocytes produce thromboxane A2-like activity during phagocytosis.", "content": "Homogenates of phagocytosing polymorphonuclear leukocytes obtained from rabbit peritoneum were incubated with the prostaglandin endoperoxides PGG2 or PGH2. After 2 min at 0degree C, incubation mixtures contained an increased rabbit aorta contracting activity. Ether extracts of incubation mixtures contained a substance which contracted the superfused strips of rabbit aorta and coeliac artery and had a half life which was similar to thromboxane A2. The generation of thromboxane A2-like activity from PG endoperoxides was prevented by boiling the homogenate prior to incubation, or by pretreatment with benzydamine, a drug which blocks thromboxane formation in platelets. Production of thromboxane A2-like material by leukocyte homogenates was compared with platelet microsomal thromboxane synthetase.", "contents": "Polymorphonuclear leukocytes produce thromboxane A2-like activity during phagocytosis. Homogenates of phagocytosing polymorphonuclear leukocytes obtained from rabbit peritoneum were incubated with the prostaglandin endoperoxides PGG2 or PGH2. After 2 min at 0degree C, incubation mixtures contained an increased rabbit aorta contracting activity. Ether extracts of incubation mixtures contained a substance which contracted the superfused strips of rabbit aorta and coeliac artery and had a half life which was similar to thromboxane A2. The generation of thromboxane A2-like activity from PG endoperoxides was prevented by boiling the homogenate prior to incubation, or by pretreatment with benzydamine, a drug which blocks thromboxane formation in platelets. Production of thromboxane A2-like material by leukocyte homogenates was compared with platelet microsomal thromboxane synthetase.", "PMID": 981700}
{"id": "pubmed23n0032_12721", "title": "Radioimmunoassay for urinary metabolites of prostaglandin F2alpha.", "content": "Antibodies against the main urinary metabolite of PGF2alpha in the human, 5alpha, 7alpha-dihydroxy-11-ketotetranorprosta-1,16-dioic acid, were raised in rabbits. The compound was coupled selectively in the omega position to bovine serum albumin prior to injection. The resuling antibodies did not distinguish between tetranor compounds varying only in structure at the omega carbon, and thus the assay could be used also for other metabolites of PGF2alpha, e.g. the main urinary metabolite in the guinea pig, 5alpha,7alpha-dihydroxy-11-ketotretranorporstanoic acid. Labeled ligands for the assays were prepared either in vivo by injection of [17, 18-3H]-PGF2alpha into humans after several days treatment with indomethacin, or in vitro by incubation of [17, 18-3H]-15-keto-13, 14-dihydro-PGF2alpha with mitochondria from rat liver. The sensitivity of the assay was 10 pg or 4 pg with these two preparations, respectively. The assay was employed for a number of measurements: normal daily excretion in a number of humans; excretion of urinary metabolites during treatment with prostaglandin synthetase inhibitors in human subjects, or after intravenous injection of PGF2alpha; excretion during human pregnancy; and prostaglandin production in the guinea pig during normal estrous cycles and pregnancies and after estrogen treatment. The results of these studies were in several cases compared to similar measurements earlier performed using mass spectrometric methods, and were found to agree well. Thus, this radioimmunoassay provides a simple and accurate method for estimating prostaglandin production, particularly suitable for long-term studies and for cases where repeated blood sampling must be avoided.", "contents": "Radioimmunoassay for urinary metabolites of prostaglandin F2alpha. Antibodies against the main urinary metabolite of PGF2alpha in the human, 5alpha, 7alpha-dihydroxy-11-ketotetranorprosta-1,16-dioic acid, were raised in rabbits. The compound was coupled selectively in the omega position to bovine serum albumin prior to injection. The resuling antibodies did not distinguish between tetranor compounds varying only in structure at the omega carbon, and thus the assay could be used also for other metabolites of PGF2alpha, e.g. the main urinary metabolite in the guinea pig, 5alpha,7alpha-dihydroxy-11-ketotretranorporstanoic acid. Labeled ligands for the assays were prepared either in vivo by injection of [17, 18-3H]-PGF2alpha into humans after several days treatment with indomethacin, or in vitro by incubation of [17, 18-3H]-15-keto-13, 14-dihydro-PGF2alpha with mitochondria from rat liver. The sensitivity of the assay was 10 pg or 4 pg with these two preparations, respectively. The assay was employed for a number of measurements: normal daily excretion in a number of humans; excretion of urinary metabolites during treatment with prostaglandin synthetase inhibitors in human subjects, or after intravenous injection of PGF2alpha; excretion during human pregnancy; and prostaglandin production in the guinea pig during normal estrous cycles and pregnancies and after estrogen treatment. The results of these studies were in several cases compared to similar measurements earlier performed using mass spectrometric methods, and were found to agree well. Thus, this radioimmunoassay provides a simple and accurate method for estimating prostaglandin production, particularly suitable for long-term studies and for cases where repeated blood sampling must be avoided.", "PMID": 981701}
{"id": "pubmed23n0032_12722", "title": "Chloroquine, quinine, procaine, quinidine and clomipramine are prostaglandin agonists and antagonists.", "content": "Chloroquine, quinine, procaine, quinidine and clomipramine behave as prostaglandin (PG) antagonists in a rat mesenteric vascular bed preparation. The ID50 concentrations were within the range of therapeutically effective human plasma levels in each case. Antagonism to PGE2 was studied in detail and seemed to be at least in part competitive. The drugs also antagonized the effects of PGs A1, A2, F2alpha and E1. Each drug also had weak prostaglandin agonist activity but only over a very narrow range of concentrations. It is possible that some of the clinical actions of these drugs may depend on blockade or imitation of natural PG effects. The findings suggest new approaches to the search for PG antagonists, a new screening technique for anti-inflammatory drugs and possible new uses for these established drugs. A preliminary study suggests that chloroquine may be successful in closing a patent ductus arteriosus in infants.", "contents": "Chloroquine, quinine, procaine, quinidine and clomipramine are prostaglandin agonists and antagonists. Chloroquine, quinine, procaine, quinidine and clomipramine behave as prostaglandin (PG) antagonists in a rat mesenteric vascular bed preparation. The ID50 concentrations were within the range of therapeutically effective human plasma levels in each case. Antagonism to PGE2 was studied in detail and seemed to be at least in part competitive. The drugs also antagonized the effects of PGs A1, A2, F2alpha and E1. Each drug also had weak prostaglandin agonist activity but only over a very narrow range of concentrations. It is possible that some of the clinical actions of these drugs may depend on blockade or imitation of natural PG effects. The findings suggest new approaches to the search for PG antagonists, a new screening technique for anti-inflammatory drugs and possible new uses for these established drugs. A preliminary study suggests that chloroquine may be successful in closing a patent ductus arteriosus in infants.", "PMID": 981703}
{"id": "pubmed23n0032_12723", "title": "Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3: inhibition by cytochalasin B.", "content": "Cytochalasin B inhibits the production of prostaglandins by serum-, thrombin-, and bradykinin-stimulated MC5-5 cells. The serum-stimulated release of arachidonic acid from cellular phospholipids also is inhibited. Cytochalasin B does not affect the cells' prostaglandin synthetase activity when exogenous arachidonic acid is present. Deacylation of phospholipids may be the step affected by cytochalasin B possibly as a result of disruption of microfilament organization. Colchicine and vinblastine, two drugs that can disrupt microtubule organization, do not inhibit prostaglandin production by cells.", "contents": "Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3: inhibition by cytochalasin B. Cytochalasin B inhibits the production of prostaglandins by serum-, thrombin-, and bradykinin-stimulated MC5-5 cells. The serum-stimulated release of arachidonic acid from cellular phospholipids also is inhibited. Cytochalasin B does not affect the cells' prostaglandin synthetase activity when exogenous arachidonic acid is present. Deacylation of phospholipids may be the step affected by cytochalasin B possibly as a result of disruption of microfilament organization. Colchicine and vinblastine, two drugs that can disrupt microtubule organization, do not inhibit prostaglandin production by cells.", "PMID": 981704}
{"id": "pubmed23n0032_12724", "title": "Effect of aspirin on prostaglandin synthesis by human platelets.", "content": "When platelet rich plasma is exposed to N-ethylmaleimide, a ten fold increase in measurable prostaglandin E synthesis occurs. This effect is almost completely abolished within 2 hours of ingestion of 600 mg of aspirin by human volunteers. Recovery of this platelet function is slow for the first two days, returning sharply to normal over the next six days and plateauing approximately 8 days following initial removal from aspirin. It is suggested from these studies that platelet prostaglandin E production following NEM may be a useful test of platelet function.", "contents": "Effect of aspirin on prostaglandin synthesis by human platelets. When platelet rich plasma is exposed to N-ethylmaleimide, a ten fold increase in measurable prostaglandin E synthesis occurs. This effect is almost completely abolished within 2 hours of ingestion of 600 mg of aspirin by human volunteers. Recovery of this platelet function is slow for the first two days, returning sharply to normal over the next six days and plateauing approximately 8 days following initial removal from aspirin. It is suggested from these studies that platelet prostaglandin E production following NEM may be a useful test of platelet function.", "PMID": 981705}
{"id": "pubmed23n0032_12725", "title": "The effect of salicylate on prostaglandin levels in rabbit knees following inducement of osteoarthritic changes.", "content": "Surgical inducement of medial instability in the right knee of rabbits was used to produce joint changes which resemble those observed in human osteoarthritis. Ordinary tap water was supplied to half of the rabbits and tap water plus sodium salicylate to the others. Determinations of prostaglandin were made on the synovial fluid and cartilage from all rabbits five months after surgery. In both groups, the concentration of prostaglandin in synovial fluid was lower in the operated knees, but the total amount of prostaglandin was found to be approximately equal to that in the unoperated knees. The development of degenerative joint changes therefore was not accompanied by increases in prostaglandin content. Salicylate treatment did not alter this observation, however, it did reduce overall prostaglandin levels. These results suggest that prostaglandin interaction is not involved in osteoarthritic joint degeneration.", "contents": "The effect of salicylate on prostaglandin levels in rabbit knees following inducement of osteoarthritic changes. Surgical inducement of medial instability in the right knee of rabbits was used to produce joint changes which resemble those observed in human osteoarthritis. Ordinary tap water was supplied to half of the rabbits and tap water plus sodium salicylate to the others. Determinations of prostaglandin were made on the synovial fluid and cartilage from all rabbits five months after surgery. In both groups, the concentration of prostaglandin in synovial fluid was lower in the operated knees, but the total amount of prostaglandin was found to be approximately equal to that in the unoperated knees. The development of degenerative joint changes therefore was not accompanied by increases in prostaglandin content. Salicylate treatment did not alter this observation, however, it did reduce overall prostaglandin levels. These results suggest that prostaglandin interaction is not involved in osteoarthritic joint degeneration.", "PMID": 981706}
{"id": "pubmed23n0032_12726", "title": "Metabolism of PG in man: effect of furosemide on the excretion of the main metabolite of PG F2alpha.", "content": "The excretion rates of main urinary metabolite of PG F2alpha were measured radioimmunologically in 4 healthy persons and in 13 essential hypertensives. The resting values were 9.3+/-0.73 in the former and 10.4+/-2.17 ng/min in the latter. There was no significant differences between them. The excretion of the metabolite decreased prominently after the administration of furosemide. The percent decrease was 57% in healthy persons and 70% in essential hypertension. The percent result supports that furosemide inhibit the catabolism of PG F2alpha.", "contents": "Metabolism of PG in man: effect of furosemide on the excretion of the main metabolite of PG F2alpha. The excretion rates of main urinary metabolite of PG F2alpha were measured radioimmunologically in 4 healthy persons and in 13 essential hypertensives. The resting values were 9.3+/-0.73 in the former and 10.4+/-2.17 ng/min in the latter. There was no significant differences between them. The excretion of the metabolite decreased prominently after the administration of furosemide. The percent decrease was 57% in healthy persons and 70% in essential hypertension. The percent result supports that furosemide inhibit the catabolism of PG F2alpha.", "PMID": 981707}
{"id": "pubmed23n0032_12727", "title": "The simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2 and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts.", "content": "The relative stability of Prostaglandins (PGs) E1, E2 and F1alpha in cultures of BALB/c 3T3 and SV3T3 cells has been evaluated using 3 different approaches. First, total recovery of tritium in the ethyl acetate phase following incubation and extraction of PGF1alpha and PGE1 demonstrated greater stability for PGF1alpha (88.8%) than PGE1 (65.9%). Second, analysis of incubated, extracted, tritiated PGs by thin layer chromatography revealed decreases of up to 23% in the PGE zone following incubation of 3H-PGE1. With increasing time of incubation, decreases in the PGE zone were accompanied by increase in PGA-like compounds. 3H-PGF1alpha demonstrated greater stability, having greater than 90% recovery of the tritium in the PGF zone. A third approach to the assessment of PG stability in culture was the comparison of the production of individual PGs by radioimmunoassay (RIA). The data obtained by RIA indicated a lag in the increase of PGA and PGB, until an initial rise in PGE was noted, suggesting that PGA and PGB may be secondary products arising from PGE which exhibits only partial stability in culture. By employing two RIAs, one for total PGE and one for PGA and PGB, the composite determination PG [E + (A + B)] can be used to provide a more meaningful determination of PG production because of the instability of the PGs. On the other hand, individual determinations are helpful in assessing the stability of PGEs in cell cultures.", "contents": "The simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2 and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts. The relative stability of Prostaglandins (PGs) E1, E2 and F1alpha in cultures of BALB/c 3T3 and SV3T3 cells has been evaluated using 3 different approaches. First, total recovery of tritium in the ethyl acetate phase following incubation and extraction of PGF1alpha and PGE1 demonstrated greater stability for PGF1alpha (88.8%) than PGE1 (65.9%). Second, analysis of incubated, extracted, tritiated PGs by thin layer chromatography revealed decreases of up to 23% in the PGE zone following incubation of 3H-PGE1. With increasing time of incubation, decreases in the PGE zone were accompanied by increase in PGA-like compounds. 3H-PGF1alpha demonstrated greater stability, having greater than 90% recovery of the tritium in the PGF zone. A third approach to the assessment of PG stability in culture was the comparison of the production of individual PGs by radioimmunoassay (RIA). The data obtained by RIA indicated a lag in the increase of PGA and PGB, until an initial rise in PGE was noted, suggesting that PGA and PGB may be secondary products arising from PGE which exhibits only partial stability in culture. By employing two RIAs, one for total PGE and one for PGA and PGB, the composite determination PG [E + (A + B)] can be used to provide a more meaningful determination of PG production because of the instability of the PGs. On the other hand, individual determinations are helpful in assessing the stability of PGEs in cell cultures.", "PMID": 981708}
{"id": "pubmed23n0032_12728", "title": "A sensitive method for the determination of amitriptyline and nortriptyline in human plasma.", "content": "A highly sensitive and reproducible GLC technique for the quantitation of amitriptyline and nortriptyline is described. The lower limit of detection is less than 1 ng/ml. 5 ng/ml samples (3 ml aliquots) can be accurately quantified (C of V less than 5%). Steady state data are reported together with data on amitriptyline and nortriptyline levels obtained in a volunteer who ingested a single oral dose (25 mg) of amitriptyline (Elavil).", "contents": "A sensitive method for the determination of amitriptyline and nortriptyline in human plasma. A highly sensitive and reproducible GLC technique for the quantitation of amitriptyline and nortriptyline is described. The lower limit of detection is less than 1 ng/ml. 5 ng/ml samples (3 ml aliquots) can be accurately quantified (C of V less than 5%). Steady state data are reported together with data on amitriptyline and nortriptyline levels obtained in a volunteer who ingested a single oral dose (25 mg) of amitriptyline (Elavil).", "PMID": 981709}
{"id": "pubmed23n0032_12729", "title": "Non-selective enhancement of locus coeruleus and substantia nigra self-stimulation after termination of chronic dopaminergic receptor blockade with pimozide in rats.", "content": "Self-stimulation of substantia nigra and locus coeruleus were assessed before and after an 8-day regimen in which pimozide was given twice daily at a dose of 0.5 mg/kg (first 4 days) or 1.0 mg/kg (last 4 days). At 48 hours after termination of pimozide treatment self-stimulation was increased to 25% above pre-pimozide baseline levels: this was true for both self-stimulation sites. Rates remained high the following day but returned to normal by the third day of post-pimozide-testing. These data are interpreted as reflecting pimozide-induced supersensitivity in a dopamenergic substrate. This substrate appears to be critical for intracranial self-stimulation even when its fibers are not themselves activated at the tip of the stimulating electrode.", "contents": "Non-selective enhancement of locus coeruleus and substantia nigra self-stimulation after termination of chronic dopaminergic receptor blockade with pimozide in rats. Self-stimulation of substantia nigra and locus coeruleus were assessed before and after an 8-day regimen in which pimozide was given twice daily at a dose of 0.5 mg/kg (first 4 days) or 1.0 mg/kg (last 4 days). At 48 hours after termination of pimozide treatment self-stimulation was increased to 25% above pre-pimozide baseline levels: this was true for both self-stimulation sites. Rates remained high the following day but returned to normal by the third day of post-pimozide-testing. These data are interpreted as reflecting pimozide-induced supersensitivity in a dopamenergic substrate. This substrate appears to be critical for intracranial self-stimulation even when its fibers are not themselves activated at the tip of the stimulating electrode.", "PMID": 981710}
{"id": "pubmed23n0032_12730", "title": "Effects of undrugged partners on scopolamine-induced changes in activity and sociability.", "content": "One group of 6 rats was injected with saline, one with 1.0 mg/Kg scopolamine (D/D) and two others had half the members in each injected with saline and half with scopolamine (S/D). Each was tested in a 36 X 36 in. open field for 10 min. The frequency of ambulation, rearing, social contacts, and the shortest and perimeter distances between rats were found. The presence of the partners in the S/D groups eliminated most of the scopolamine-induced changes found in the D/D group, showing that the nature of the social environment affects drug-induced changes in activity and sociability.", "contents": "Effects of undrugged partners on scopolamine-induced changes in activity and sociability. One group of 6 rats was injected with saline, one with 1.0 mg/Kg scopolamine (D/D) and two others had half the members in each injected with saline and half with scopolamine (S/D). Each was tested in a 36 X 36 in. open field for 10 min. The frequency of ambulation, rearing, social contacts, and the shortest and perimeter distances between rats were found. The presence of the partners in the S/D groups eliminated most of the scopolamine-induced changes found in the D/D group, showing that the nature of the social environment affects drug-induced changes in activity and sociability.", "PMID": 981711}
{"id": "pubmed23n0032_12731", "title": "Pharmacokinetics of lithium in human plasma and erythrocytes.", "content": "The time course of lithium concentration in plasma and RBCs was measured in normal adult males following administration of single and multiple doses of lithium carbonate. From the single dose profiles, it was determined that lithium distribution between plasma and RBCs is not a simple partitioning phenomenon. The single dose time course measurements in both blood components were fit to a two compartment pharmacokinetic model in which the plasma was representative of the central compartment and the RBCs were representative of the tissue compartment. The importance of correction for trapped plasma volume in studies measuring lithium RBC kinetics was emphasized. In this study it was also demonstrated that one can accurately predict plasma and RBC lithium concentrations which are observed following multiple dosing, on the basis of single dose parameters obtained in the same subject.", "contents": "Pharmacokinetics of lithium in human plasma and erythrocytes. The time course of lithium concentration in plasma and RBCs was measured in normal adult males following administration of single and multiple doses of lithium carbonate. From the single dose profiles, it was determined that lithium distribution between plasma and RBCs is not a simple partitioning phenomenon. The single dose time course measurements in both blood components were fit to a two compartment pharmacokinetic model in which the plasma was representative of the central compartment and the RBCs were representative of the tissue compartment. The importance of correction for trapped plasma volume in studies measuring lithium RBC kinetics was emphasized. In this study it was also demonstrated that one can accurately predict plasma and RBC lithium concentrations which are observed following multiple dosing, on the basis of single dose parameters obtained in the same subject.", "PMID": 981712}
{"id": "pubmed23n0032_12732", "title": "Erythrocyte packing rates and altered lysolecithin levels during pregnancy.", "content": "The rate of packing of erythrocytes during centrifugation has been measured as an index of erythrocyte flexibility. Exposure of erythrocytes to sub-haemolytic concentrations of lysolecithin decreased their packing rate indicating that the cells had become less flexible. Erythrocyte packing rates and plasma lysolecithin levels have been measured in a series of forty pregnant and non-pregnant women. The plasma concentrations of lysolecithin were significantly lower in pregnant women when compared with non-pregnant women. In contrast to this, the plasma concentrations of total phospholipid and lecithin were elevated during pregnancy. Erythrocyte packing rates were increased during pregnancy and a statistically very significant negative correlation was found between erythrocyte packing rate and plasma lysolecithin concentration.", "contents": "Erythrocyte packing rates and altered lysolecithin levels during pregnancy. The rate of packing of erythrocytes during centrifugation has been measured as an index of erythrocyte flexibility. Exposure of erythrocytes to sub-haemolytic concentrations of lysolecithin decreased their packing rate indicating that the cells had become less flexible. Erythrocyte packing rates and plasma lysolecithin levels have been measured in a series of forty pregnant and non-pregnant women. The plasma concentrations of lysolecithin were significantly lower in pregnant women when compared with non-pregnant women. In contrast to this, the plasma concentrations of total phospholipid and lecithin were elevated during pregnancy. Erythrocyte packing rates were increased during pregnancy and a statistically very significant negative correlation was found between erythrocyte packing rate and plasma lysolecithin concentration.", "PMID": 981739}
{"id": "pubmed23n0032_12733", "title": "Action of methoxyflurane on intraocular pressure (IOP) and acid-basic equilibrium in the dog.", "content": "MEthoxyflurane pulmonary inhalation administered to dogs, 2 ml/10kg/5 min, after 10 minutes caused a significant increase (13.3 mmHg) in intraocular pressure as well (6 mmHg) in venous pressure and a significant fall, of 23.3 mmHg, in arterial pressure and in respiratory frequency, of 3.2 per min and amplitude of 7.1 mm. On the other side, as acid-basic equilibrium alterations in blood, were observed a fall of 0.13 of pH and an increase of 18.3 mmHg of pCO2 and of 2.7 mEq/l of bicarbonate concentration.", "contents": "Action of methoxyflurane on intraocular pressure (IOP) and acid-basic equilibrium in the dog. MEthoxyflurane pulmonary inhalation administered to dogs, 2 ml/10kg/5 min, after 10 minutes caused a significant increase (13.3 mmHg) in intraocular pressure as well (6 mmHg) in venous pressure and a significant fall, of 23.3 mmHg, in arterial pressure and in respiratory frequency, of 3.2 per min and amplitude of 7.1 mm. On the other side, as acid-basic equilibrium alterations in blood, were observed a fall of 0.13 of pH and an increase of 18.3 mmHg of pCO2 and of 2.7 mEq/l of bicarbonate concentration.", "PMID": 981740}
{"id": "pubmed23n0032_12734", "title": "[Glucose tolerance tests with oral glucose challenges of 50 and 100 grams (author's transl)].", "content": "Seventy six subjects (63 females and 13 males) with an average age of 50.5 years, all of them with familial and/or obstetric history for diabetes mellitus, were submitted to glucose tolerance tests with oral glucose challenges of 50 and 100 g. The tests were all analysed by different criteria of current usage in medical literature (Wilkerson, Fajans and Conn, British Diabetes Association and University Group Diabetes Program). We concluded that the 100 g glucose challenge gives a greater index of positivity by all the criteria above referred when compared to 50 g challenge. Wilkerson's criteria is less sensitive than the others, when applied to 100 g glucose challenges.", "contents": "[Glucose tolerance tests with oral glucose challenges of 50 and 100 grams (author's transl)]. Seventy six subjects (63 females and 13 males) with an average age of 50.5 years, all of them with familial and/or obstetric history for diabetes mellitus, were submitted to glucose tolerance tests with oral glucose challenges of 50 and 100 g. The tests were all analysed by different criteria of current usage in medical literature (Wilkerson, Fajans and Conn, British Diabetes Association and University Group Diabetes Program). We concluded that the 100 g glucose challenge gives a greater index of positivity by all the criteria above referred when compared to 50 g challenge. Wilkerson's criteria is less sensitive than the others, when applied to 100 g glucose challenges.", "PMID": 981741}
{"id": "pubmed23n0032_12735", "title": "Isolation of a new lipolytic-melanotropic peptide from human pituitary glands.", "content": "A new peptide having both lipolytic and melanotropic properties has been isolated from human pituitary glands. It has a molecular weight around 11,000, an amino acid composition different from the known lipolytic-melanotropic hormones, and an isoelectric point of 8.5. Although it is not entirely pure, there is no doubt that it differs from other known lipolytic-melanotropic substances in its total lack of methionine and tryptophan and its unusually high content of lysine and histidine. It has a melanocyte-stimulating hormone activity of 19 units/mug. As a lipolytic agent it is active with rabbit adipocytes, only slightly active with human cells and inactive with adipocytes from the rat.", "contents": "Isolation of a new lipolytic-melanotropic peptide from human pituitary glands. A new peptide having both lipolytic and melanotropic properties has been isolated from human pituitary glands. It has a molecular weight around 11,000, an amino acid composition different from the known lipolytic-melanotropic hormones, and an isoelectric point of 8.5. Although it is not entirely pure, there is no doubt that it differs from other known lipolytic-melanotropic substances in its total lack of methionine and tryptophan and its unusually high content of lysine and histidine. It has a melanocyte-stimulating hormone activity of 19 units/mug. As a lipolytic agent it is active with rabbit adipocytes, only slightly active with human cells and inactive with adipocytes from the rat.", "PMID": 981742}
{"id": "pubmed23n0032_12736", "title": "Base competition of DNA isolated from Thiobacillus ferrooxidans grown on different substrates.", "content": "The present study indicates some anomalies with respect to the DNA base composition of Thiobacillus ferrooxidans when it is cultured on different substrates. The % GC of the DNA of this bacterium has been calculated by three different methods (melting temperature, CsCl density gradient centrifugation and ultra-violet absorbancy ratios) using Escherichia coli and Rhodospirillum rubrum as references. The main values for T. ferrooxidans grown on ferrous iron, chalcopyrite and lead sulfide concentrates were calculated to be 56.0, 60.1 and 54.4% GC respectively. Although these large differences are not completely understood, an attempt has been made to explain this phenomenon.", "contents": "Base competition of DNA isolated from Thiobacillus ferrooxidans grown on different substrates. The present study indicates some anomalies with respect to the DNA base composition of Thiobacillus ferrooxidans when it is cultured on different substrates. The % GC of the DNA of this bacterium has been calculated by three different methods (melting temperature, CsCl density gradient centrifugation and ultra-violet absorbancy ratios) using Escherichia coli and Rhodospirillum rubrum as references. The main values for T. ferrooxidans grown on ferrous iron, chalcopyrite and lead sulfide concentrates were calculated to be 56.0, 60.1 and 54.4% GC respectively. Although these large differences are not completely understood, an attempt has been made to explain this phenomenon.", "PMID": 981743}
{"id": "pubmed23n0032_12737", "title": "Immediate effects of TSH on the cavian thyroid.", "content": "Which stage of hormone synthesis is stimulated as the immediate response of the thyroid to thyrotropin (thyroid stimulating hormone, TSH)? Is there specific binding of TSH to thyroid tissue? Experiments were done with thyroids taken from propylthiouracil-treated guinea pigs to attempt to answer these questions. Such thyroids take up radioiodide (after 35 min in vitro; 10% of the total added; after 24 hours in vivo: 20% of the amount given)and, being only partially blocked, can utilize this iodide for hormone synthesis. Following pre-incubation (30 sec with or without TSH), incubation in Geys medium/air, proteolysis of the tissue and/or extraction of the incubation medium, tha iodinated compounds were isolated. These compounds were separated by thin layer chromatography and were either quantitated by cutting and counting the plates, or located by spraying the plates with the ferricyanide-ferrichloride-arsenic acid (FFCA) reagent. For iodide, similar procedures were used, on the same plates. This in vitro preparation, using partially blocked thyroid tissue, can serve as a model to detect slight alterations in iodide metabolism of the thyroid. TSH appears to bind specifically to thyroid tissue (more of the 125 I added was taken up in vitro by thyroid than by a control tissue). Finally, since after a 30-sec pre-incubation with TSH (control, no TSH), the tissue contained more DIT, less T3 and since less of the 125I taken up appeared as iodide, hormone synthesis and secretion appear to be the earliest responses of thyroid tissue to stimulation by TSH.", "contents": "Immediate effects of TSH on the cavian thyroid. Which stage of hormone synthesis is stimulated as the immediate response of the thyroid to thyrotropin (thyroid stimulating hormone, TSH)? Is there specific binding of TSH to thyroid tissue? Experiments were done with thyroids taken from propylthiouracil-treated guinea pigs to attempt to answer these questions. Such thyroids take up radioiodide (after 35 min in vitro; 10% of the total added; after 24 hours in vivo: 20% of the amount given)and, being only partially blocked, can utilize this iodide for hormone synthesis. Following pre-incubation (30 sec with or without TSH), incubation in Geys medium/air, proteolysis of the tissue and/or extraction of the incubation medium, tha iodinated compounds were isolated. These compounds were separated by thin layer chromatography and were either quantitated by cutting and counting the plates, or located by spraying the plates with the ferricyanide-ferrichloride-arsenic acid (FFCA) reagent. For iodide, similar procedures were used, on the same plates. This in vitro preparation, using partially blocked thyroid tissue, can serve as a model to detect slight alterations in iodide metabolism of the thyroid. TSH appears to bind specifically to thyroid tissue (more of the 125 I added was taken up in vitro by thyroid than by a control tissue). Finally, since after a 30-sec pre-incubation with TSH (control, no TSH), the tissue contained more DIT, less T3 and since less of the 125I taken up appeared as iodide, hormone synthesis and secretion appear to be the earliest responses of thyroid tissue to stimulation by TSH.", "PMID": 981744}
{"id": "pubmed23n0032_12738", "title": "Biochemical changes in Pinus pinea seeds. III. The effect of growth substances and steroidal hormones on nucleic acids.", "content": "The effects of exogenous growth factors (indolacetic acid, gibberellic acid and kinetin and steroidal hormones (estrone, estradiol and testosterone on the germination pattern of Pinus pinea seeds were studied. Nucleic acids metabolism during the stages before germination, has also been investigated. Seeds sown in the presence  of all these substances, showed a higher growth rate and a higher germination degree than their respective controls; kinetin and estradiol were the most active factors. The level of total nucleic acids was studied in seeds after one day soaking and on the 1rst, 3rd, 5th, and 7th day after sowing. Megagametophytes contained a higher amount of acids when growth factors and steroidal hormones were present, specially after one day soaking. In embryos; after the 3rd day, a similar result was obtained. The RNA fraction increased the most.", "contents": "Biochemical changes in Pinus pinea seeds. III. The effect of growth substances and steroidal hormones on nucleic acids. The effects of exogenous growth factors (indolacetic acid, gibberellic acid and kinetin and steroidal hormones (estrone, estradiol and testosterone on the germination pattern of Pinus pinea seeds were studied. Nucleic acids metabolism during the stages before germination, has also been investigated. Seeds sown in the presence  of all these substances, showed a higher growth rate and a higher germination degree than their respective controls; kinetin and estradiol were the most active factors. The level of total nucleic acids was studied in seeds after one day soaking and on the 1rst, 3rd, 5th, and 7th day after sowing. Megagametophytes contained a higher amount of acids when growth factors and steroidal hormones were present, specially after one day soaking. In embryos; after the 3rd day, a similar result was obtained. The RNA fraction increased the most.", "PMID": 981778}
{"id": "pubmed23n0032_12739", "title": "[Serum ige levels in healthy children (author's transl)].", "content": "Serum IgE levels have been measured in a group of 55 healthy children from 2 to 10 years of age. The IgE mean value increases with the age but no sexual differences have been found. The numerical distribution of the different genotypes observed in the unselected population, follows the Hardy-Weinberg law, which describes the segregation of two alleles at a single locus.", "contents": "[Serum ige levels in healthy children (author's transl)]. Serum IgE levels have been measured in a group of 55 healthy children from 2 to 10 years of age. The IgE mean value increases with the age but no sexual differences have been found. The numerical distribution of the different genotypes observed in the unselected population, follows the Hardy-Weinberg law, which describes the segregation of two alleles at a single locus.", "PMID": 981779}
{"id": "pubmed23n0032_12740", "title": "Effects of sympathetic stimulation on carotid and aortic baroreceptors in the cat.", "content": "Variations in the discharge of baroreceptor units in the left aortic nerve were investigated during stimulation of the stellate ganglion both in the intact cat and in vitro perfusion of the aortic arch. The effects of stimulation of the peripheral cervical sympathetic trunk of the baroreceptor discharge in the carotid nerve during in vitro perfusion of the carotid artery were further studied. Stimulation of the stellate ganglion or the aortic nerve in the intact cat caused a simultaneous increase in arterial pressure, heart rate and number of baroreceptor impulses in a filament to the left aortic nerve. In the in vitro studies, decreases in the number of baroreceptor impulses both in the aortic and carotid nerves were produced in most cases during the stimulation of the sympathetic nerves. These effects were only observed during low pressure perfusion of the isolated artery and in low frequency changes. The infusion of norepinephrine caused a more marked decrease.", "contents": "Effects of sympathetic stimulation on carotid and aortic baroreceptors in the cat. Variations in the discharge of baroreceptor units in the left aortic nerve were investigated during stimulation of the stellate ganglion both in the intact cat and in vitro perfusion of the aortic arch. The effects of stimulation of the peripheral cervical sympathetic trunk of the baroreceptor discharge in the carotid nerve during in vitro perfusion of the carotid artery were further studied. Stimulation of the stellate ganglion or the aortic nerve in the intact cat caused a simultaneous increase in arterial pressure, heart rate and number of baroreceptor impulses in a filament to the left aortic nerve. In the in vitro studies, decreases in the number of baroreceptor impulses both in the aortic and carotid nerves were produced in most cases during the stimulation of the sympathetic nerves. These effects were only observed during low pressure perfusion of the isolated artery and in low frequency changes. The infusion of norepinephrine caused a more marked decrease.", "PMID": 981780}
{"id": "pubmed23n0032_12741", "title": "Active transport of sugars in tortoise intestine.", "content": "The tortoise intestine capability for active transport of sugars has been studied in vitro at 30 degrees C, using labelled sugars. A release of glucose from the glycogen stores of the intestinal wall to the medium took place throughout the incubation period of the sacs. An active transport of 14C-D-glucose against a concentration gradient from the mucosal to the serosal compartment was evident, whereas no such activity could be detected for 14C-D-galactose. The tissue oxygen uptake was 36% higher with glucose than with galactose in the medium.", "contents": "Active transport of sugars in tortoise intestine. The tortoise intestine capability for active transport of sugars has been studied in vitro at 30 degrees C, using labelled sugars. A release of glucose from the glycogen stores of the intestinal wall to the medium took place throughout the incubation period of the sacs. An active transport of 14C-D-glucose against a concentration gradient from the mucosal to the serosal compartment was evident, whereas no such activity could be detected for 14C-D-galactose. The tissue oxygen uptake was 36% higher with glucose than with galactose in the medium.", "PMID": 981781}
{"id": "pubmed23n0032_12742", "title": "The sub-cellular localization of stereo-specific opiate binding in mouse brain.", "content": "Of the three primary sub-cellular fractions derived from mouse brain homogenates, the P3 (microsomal) fraction binds 3H-di-hydromorphine stereo-specifically with the highest capacity per mg. of protein; this level is nearly as great as that bound by purified synaptosomal plasma membranes (SPM). The binding to P3 is unlikely to be attributable to contamination with SPM, because a) ten times as much total binding is recovered in P3 as in SPM, and b) the level of binding to P3 is highest in a sub-fraction banding above 0.8 M sucrose, rich in surface membranes of all types, whereas SPM bands preferentially at at 0.8 M sucrose, rich in surface membranes of all types, whereas SPM bands preferentially at 0.8-1.1 M sucrose. The binding of either 3H-di-hydromorphine or 3H-naloxone to P3 is, however, indistinguishable from that found in nerve endings with respect to a) its KD; b) the relative potencies of several agonists in displacing it; and c) the effects on it of Na+ or trypsin. Thus, it appears that stereo-specific opiate receptors are distributed diffusely on the entire surface of nerve cells and not concentrated at the synaptic region as has previously been supposed.", "contents": "The sub-cellular localization of stereo-specific opiate binding in mouse brain. Of the three primary sub-cellular fractions derived from mouse brain homogenates, the P3 (microsomal) fraction binds 3H-di-hydromorphine stereo-specifically with the highest capacity per mg. of protein; this level is nearly as great as that bound by purified synaptosomal plasma membranes (SPM). The binding to P3 is unlikely to be attributable to contamination with SPM, because a) ten times as much total binding is recovered in P3 as in SPM, and b) the level of binding to P3 is highest in a sub-fraction banding above 0.8 M sucrose, rich in surface membranes of all types, whereas SPM bands preferentially at at 0.8 M sucrose, rich in surface membranes of all types, whereas SPM bands preferentially at 0.8-1.1 M sucrose. The binding of either 3H-di-hydromorphine or 3H-naloxone to P3 is, however, indistinguishable from that found in nerve endings with respect to a) its KD; b) the relative potencies of several agonists in displacing it; and c) the effects on it of Na+ or trypsin. Thus, it appears that stereo-specific opiate receptors are distributed diffusely on the entire surface of nerve cells and not concentrated at the synaptic region as has previously been supposed.", "PMID": 981782}
{"id": "pubmed23n0032_12743", "title": "The comparison of fluoxetine and nisoxetine with tricyclic antidepressants in blocking the neurotoxicity of p-chloroamphetamine and 6-hydroxydopamine in the rat brain.", "content": "Fluoxetine prevents the loss of 5-hydroxytryptamine (5HT) uptake in synaptosomes of cerebral cortex after intraperitoneally administered p-chloroamphetamine (p-CA) with an ED50 of 3.8 mg/kg i.p. in rats. However, at 50 mg/kg, it does not prevent the loss of norepinephrine (NE) uptake in synaptosomes of hypothalamus after intraventricularly administered 6-hydroxydopamine (6-OHDA). Nisoxetine, on the other hand, centrally protects NE uptake from the neurotoxic effect of 6-OHDA with an ED50 value of 5 mg/kg i.p. At 50 mg/kg, it gives only 35% protection of 5HT uptake from the neurotoxic effect of p-CA. In comparison with the ED50 values of tricyclic antidepressants, both fluoxetine and nisoxetine are more potent and selective blockers of neurotoxicity resulting from the central actions of p-CA and 6-OHDA, respectively, in vivo.", "contents": "The comparison of fluoxetine and nisoxetine with tricyclic antidepressants in blocking the neurotoxicity of p-chloroamphetamine and 6-hydroxydopamine in the rat brain. Fluoxetine prevents the loss of 5-hydroxytryptamine (5HT) uptake in synaptosomes of cerebral cortex after intraperitoneally administered p-chloroamphetamine (p-CA) with an ED50 of 3.8 mg/kg i.p. in rats. However, at 50 mg/kg, it does not prevent the loss of norepinephrine (NE) uptake in synaptosomes of hypothalamus after intraventricularly administered 6-hydroxydopamine (6-OHDA). Nisoxetine, on the other hand, centrally protects NE uptake from the neurotoxic effect of 6-OHDA with an ED50 value of 5 mg/kg i.p. At 50 mg/kg, it gives only 35% protection of 5HT uptake from the neurotoxic effect of p-CA. In comparison with the ED50 values of tricyclic antidepressants, both fluoxetine and nisoxetine are more potent and selective blockers of neurotoxicity resulting from the central actions of p-CA and 6-OHDA, respectively, in vivo.", "PMID": 981783}
{"id": "pubmed23n0032_12744", "title": "Warfarin: stereochemical aspects of its metabolism in vivo in the rat.", "content": "The biotransformation of the R and S isomers of warfarin was investigated in the rat. The formation of 7-hydroxywarfarin was stereoselective for the R enantiomer, while the formation of 4'-hydroxywarfarin was stereoselective for the S enantiomer. The 6-, 8-, and benzylic hydroxylation of both isomers was approximately the same. The reduction of the side chain ketone function of warfarin to the corresponding diastereomeric warfarin alcohols was stereoselective for the S isomer. The reduction also displayed a degree of stereospecificity with S reduction occurring predominantly. The results of the in vivo study agree in many cases with a previous in vitro investigation. However, differences between the in vitro and in vivo studies do exist and suggest that secondary stereoselective biotransformation routes occur in vivo and that the microsomal and soluble enzymes employed in the in vitro study may have been disrupted during isolation. Large amounts of polar labile conjugates of R and S warfarin, and metabolites were found in the urine. The 4-hydroxyl group of the coumarin ring appears to be the position of conjugation and this process appears to be regio and stereoselective.", "contents": "Warfarin: stereochemical aspects of its metabolism in vivo in the rat. The biotransformation of the R and S isomers of warfarin was investigated in the rat. The formation of 7-hydroxywarfarin was stereoselective for the R enantiomer, while the formation of 4'-hydroxywarfarin was stereoselective for the S enantiomer. The 6-, 8-, and benzylic hydroxylation of both isomers was approximately the same. The reduction of the side chain ketone function of warfarin to the corresponding diastereomeric warfarin alcohols was stereoselective for the S isomer. The reduction also displayed a degree of stereospecificity with S reduction occurring predominantly. The results of the in vivo study agree in many cases with a previous in vitro investigation. However, differences between the in vitro and in vivo studies do exist and suggest that secondary stereoselective biotransformation routes occur in vivo and that the microsomal and soluble enzymes employed in the in vitro study may have been disrupted during isolation. Large amounts of polar labile conjugates of R and S warfarin, and metabolites were found in the urine. The 4-hydroxyl group of the coumarin ring appears to be the position of conjugation and this process appears to be regio and stereoselective.", "PMID": 981784}
{"id": "pubmed23n0032_12745", "title": "Drug interactions in the horse: effect of furosemide on plasma and urinary levels of phenylbutazone.", "content": "Horses pretreated with 6.6 mg/kg of phenylbutazone were injected with 1 mg/kg of furosemide intravenously. Furosemide had no clinically significant effect on either plasma levels or plasma half-life of phenylbutazone. Furosemide reduced urinary levels of phenylbutazone 18-fold to concentrations which may result in inconsistent drug detection in routine screening tests. The results show that it is not possible to monitor compliance with phenylbutazone medication rules by means of urinalysis alone if the use of furosemide is permitted. Furosemide treatment, however, does not interfere with monitoring by plasma level determinations.", "contents": "Drug interactions in the horse: effect of furosemide on plasma and urinary levels of phenylbutazone. Horses pretreated with 6.6 mg/kg of phenylbutazone were injected with 1 mg/kg of furosemide intravenously. Furosemide had no clinically significant effect on either plasma levels or plasma half-life of phenylbutazone. Furosemide reduced urinary levels of phenylbutazone 18-fold to concentrations which may result in inconsistent drug detection in routine screening tests. The results show that it is not possible to monitor compliance with phenylbutazone medication rules by means of urinalysis alone if the use of furosemide is permitted. Furosemide treatment, however, does not interfere with monitoring by plasma level determinations.", "PMID": 981785}
{"id": "pubmed23n0032_12746", "title": "Comparison of the drug metabolizing enzymes in the liver and kidneys from homozygous nude swiss, heterozygous normal swiss, homozygous normal Swiss and DBA/2 mice.", "content": "The hepatic and renal microsomal drug metabolizing enzyme systems were isolated from homozygous nude Swiss (nu/nu), heterozygous normal Swiss (nu/+), homozygous normal Swiss (+/+) and DBA/2 mice. Microsomal protein and cytochrome P-450 concentrations were measured and the activity of ethylmorphine demethylase, aniline hydroxylase, aryl hydrocarbon hydroxylase and UDP-glucuronyl transferase were determined. Hepatic microsomes from both experimental groups carrying the nude gene were able to metabolize aniline and ethylmorphine more rapidly (20% and 36%, respectively) than the DBA/2 or Swiss homozygous normal mice. No difference between test groups was observed for hepatic aryl hydrocarbon hydroxylase or UDP-glucuronyl transferase activity. Kidney microsomes from mice carrying the nude gene had approximately twice the aryl hydrocarbon hydroxylase activity of the other two experimental groups. Renal mixed-function oxidase pathways measured for the homozygous nude mouse showed a higher overall rate of activity than the other three experimental groups. No significant difference in renal UDP-glucuronyl transferase was observed between mouse groups.", "contents": "Comparison of the drug metabolizing enzymes in the liver and kidneys from homozygous nude swiss, heterozygous normal swiss, homozygous normal Swiss and DBA/2 mice. The hepatic and renal microsomal drug metabolizing enzyme systems were isolated from homozygous nude Swiss (nu/nu), heterozygous normal Swiss (nu/+), homozygous normal Swiss (+/+) and DBA/2 mice. Microsomal protein and cytochrome P-450 concentrations were measured and the activity of ethylmorphine demethylase, aniline hydroxylase, aryl hydrocarbon hydroxylase and UDP-glucuronyl transferase were determined. Hepatic microsomes from both experimental groups carrying the nude gene were able to metabolize aniline and ethylmorphine more rapidly (20% and 36%, respectively) than the DBA/2 or Swiss homozygous normal mice. No difference between test groups was observed for hepatic aryl hydrocarbon hydroxylase or UDP-glucuronyl transferase activity. Kidney microsomes from mice carrying the nude gene had approximately twice the aryl hydrocarbon hydroxylase activity of the other two experimental groups. Renal mixed-function oxidase pathways measured for the homozygous nude mouse showed a higher overall rate of activity than the other three experimental groups. No significant difference in renal UDP-glucuronyl transferase was observed between mouse groups.", "PMID": 981786}
{"id": "pubmed23n0032_12747", "title": "Pine oil toxicity in the horse: drug detection, residues and pathological changes.", "content": "This report concerns the detection and acute toxicity of pine oil (a commercially available disinfectant) after intravenous administration in horses. alpha Terpineol was identified as a major constituent of pine oil. alpha Terpineol was recovered from equine tissues by extraction into heptane and detected by gas chromatography, using either flame ionization detection or pentafluoropropionic anhydride derivatization and electron capture detection. After intravenous injection of 0.1 ml/kg, death due to massive pulmonary edema occurred within minutes. In this animal blood and tissue levels of alpha-terpineol of between 150 and 300 ppm were observed. After smaller doses of pine oil (0.033 ml/kg), horses survived until euthanized up to 48 hours later. Blood levels of alpha-terpineol became undetectable in one of these animals after 2 hours, and no tissue levels were detected at postmortem. Marked histopathological changes were seen in the lungs of animals which survived the initial injection period. It appears that after intravenous injection of pine oil in horses lesions are largely related to the respiratory tract, and the mechanism of death is acute pulmonary edema.", "contents": "Pine oil toxicity in the horse: drug detection, residues and pathological changes. This report concerns the detection and acute toxicity of pine oil (a commercially available disinfectant) after intravenous administration in horses. alpha Terpineol was identified as a major constituent of pine oil. alpha Terpineol was recovered from equine tissues by extraction into heptane and detected by gas chromatography, using either flame ionization detection or pentafluoropropionic anhydride derivatization and electron capture detection. After intravenous injection of 0.1 ml/kg, death due to massive pulmonary edema occurred within minutes. In this animal blood and tissue levels of alpha-terpineol of between 150 and 300 ppm were observed. After smaller doses of pine oil (0.033 ml/kg), horses survived until euthanized up to 48 hours later. Blood levels of alpha-terpineol became undetectable in one of these animals after 2 hours, and no tissue levels were detected at postmortem. Marked histopathological changes were seen in the lungs of animals which survived the initial injection period. It appears that after intravenous injection of pine oil in horses lesions are largely related to the respiratory tract, and the mechanism of death is acute pulmonary edema.", "PMID": 981787}
{"id": "pubmed23n0032_12748", "title": "Toxicologic studies of tin needles at the intrathoracic site of mice.", "content": "Male Marsh mice which received a single intrathoracic injection of 4 mg of tin needles at 3 months of age were compared with controls given isotonic saline. The form and size of most of the tin needles were similar to those of asbestos needles which produced mesotheliomas and lung neoplasms in rodents. Water and food intake was markedly reduced for 24 hrs after the injection of tin. Survival rates (followed for 19 months), pathology of the bladder, liver, and kidney, as well as local and general cancer development were not adversely associated with the tin treatment as compared with the tin treatment as compared with controls. The local reaction to the tin included giant cell phagocytosis, nodular fibroplasia, and capillary formation. The failure of the tin needles to induce local neoplasms may relate to their high density as compared with silicates and/or their lack of the unique potential of the silicate particles to stimulate avascular fibrosis.", "contents": "Toxicologic studies of tin needles at the intrathoracic site of mice. Male Marsh mice which received a single intrathoracic injection of 4 mg of tin needles at 3 months of age were compared with controls given isotonic saline. The form and size of most of the tin needles were similar to those of asbestos needles which produced mesotheliomas and lung neoplasms in rodents. Water and food intake was markedly reduced for 24 hrs after the injection of tin. Survival rates (followed for 19 months), pathology of the bladder, liver, and kidney, as well as local and general cancer development were not adversely associated with the tin treatment as compared with the tin treatment as compared with controls. The local reaction to the tin included giant cell phagocytosis, nodular fibroplasia, and capillary formation. The failure of the tin needles to induce local neoplasms may relate to their high density as compared with silicates and/or their lack of the unique potential of the silicate particles to stimulate avascular fibrosis.", "PMID": 981788}
{"id": "pubmed23n0032_12749", "title": "A rapid and sensitive method for determination of theophylline in plasma and saliva by high pressure liquid chromatography.", "content": "A fast and sensitive high pressure liquid chromatographic method for the monitoring of theophylline concentrations in plasma or saliva was developed. Each sample requires only about 15 minutes for the completion of the assay after receiving the plasma or saliva sample. Only 0.1 ml of sample is required, and concentrations as low as 1 mcg/ml can be accurately measured.", "contents": "A rapid and sensitive method for determination of theophylline in plasma and saliva by high pressure liquid chromatography. A fast and sensitive high pressure liquid chromatographic method for the monitoring of theophylline concentrations in plasma or saliva was developed. Each sample requires only about 15 minutes for the completion of the assay after receiving the plasma or saliva sample. Only 0.1 ml of sample is required, and concentrations as low as 1 mcg/ml can be accurately measured.", "PMID": 981789}
{"id": "pubmed23n0032_12750", "title": "Rapid fluorometric analysis of unbound salicylate in whole blood.", "content": "A method has been developed for the analysis of unbound salicylate by membrane cone ultrafiltration of either whole blood or plasma with quantitation by spectrofluorometry. The method was used to study the distribution of salicylate in whole blood over a wide range of concentrations. It was found that at a concentration of 50 ug/ml in whole blood only 9% of the salicylate is present as free drug in the plasma water, while at a concentration of 500 ug/ml, 23% is free. At concentrations exceeding 500 ug/ml the plasma protein binding sites appear to be fully satrated since the free drug concentration increases linearly with respect to the whole blood concentration. Changes in hematocrit have a relatively minor effect on the ultrafiltrate salicylate concentration. The blood/plasma salicylate concentration ratio was found to vary widely over the blood salicylate range studied, 50-1000 ug/ml.", "contents": "Rapid fluorometric analysis of unbound salicylate in whole blood. A method has been developed for the analysis of unbound salicylate by membrane cone ultrafiltration of either whole blood or plasma with quantitation by spectrofluorometry. The method was used to study the distribution of salicylate in whole blood over a wide range of concentrations. It was found that at a concentration of 50 ug/ml in whole blood only 9% of the salicylate is present as free drug in the plasma water, while at a concentration of 500 ug/ml, 23% is free. At concentrations exceeding 500 ug/ml the plasma protein binding sites appear to be fully satrated since the free drug concentration increases linearly with respect to the whole blood concentration. Changes in hematocrit have a relatively minor effect on the ultrafiltrate salicylate concentration. The blood/plasma salicylate concentration ratio was found to vary widely over the blood salicylate range studied, 50-1000 ug/ml.", "PMID": 981790}
{"id": "pubmed23n0032_12751", "title": "Effect of propranolol on rat brain norepinephrine in vitro.", "content": "At a 10(-5) to 10(-3) molar concentration range, 1-, d1- and d-propranolol were equipotent to progressively inhibit the uptake of 3H-NE into chopped rat cerebral cortex tissue. Similar concentrations of 1- and d1-, but not d-, propranolol significantly increased the amount of total deaminated catabolites (deaminated, deaminated-O-methylated) of 3H-NE; however, 10(-3) molar 1-, d1- and d-propranolol were required to increase the release of 3H-NE from rat brain in vitro.", "contents": "Effect of propranolol on rat brain norepinephrine in vitro. At a 10(-5) to 10(-3) molar concentration range, 1-, d1- and d-propranolol were equipotent to progressively inhibit the uptake of 3H-NE into chopped rat cerebral cortex tissue. Similar concentrations of 1- and d1-, but not d-, propranolol significantly increased the amount of total deaminated catabolites (deaminated, deaminated-O-methylated) of 3H-NE; however, 10(-3) molar 1-, d1- and d-propranolol were required to increase the release of 3H-NE from rat brain in vitro.", "PMID": 981791}
{"id": "pubmed23n0032_12752", "title": "Increased phenytoin metabolism in rabbits after phenytoin pretreatment.", "content": "The serum half-life of phenytoin in rabbits after a single intravenous dose was significantly shortened by pretreating the rabbits with daily oral doses of phenytoin. This suggests that in vivo phenytoin induces its own metabolism.", "contents": "Increased phenytoin metabolism in rabbits after phenytoin pretreatment. The serum half-life of phenytoin in rabbits after a single intravenous dose was significantly shortened by pretreating the rabbits with daily oral doses of phenytoin. This suggests that in vivo phenytoin induces its own metabolism.", "PMID": 981792}
{"id": "pubmed23n0032_12753", "title": "Effect of naproxen on protein binding of warfarin in human serum.", "content": "The effect of the non-steroidal anti-inflammatory agent naproxen on the protein binding of racemic warfarin in pooled human serum was determined by equilibrium dialysis. The free fraction of warfarin in serum increased practically linearly with increasing naproxen concentration. At the highest naproxen concentration tested (400 mcg/ml), the warfarin free fraction was 74% larger than the control free fraction value.", "contents": "Effect of naproxen on protein binding of warfarin in human serum. The effect of the non-steroidal anti-inflammatory agent naproxen on the protein binding of racemic warfarin in pooled human serum was determined by equilibrium dialysis. The free fraction of warfarin in serum increased practically linearly with increasing naproxen concentration. At the highest naproxen concentration tested (400 mcg/ml), the warfarin free fraction was 74% larger than the control free fraction value.", "PMID": 981793}
{"id": "pubmed23n0032_12754", "title": "Binding of labeled N-methyl N-nitrosourea to macromolecules of squamous and glandular stomach of rats.", "content": "The binding of labeled N-methyl-N-nitrosourea (3H-MNU) to macromolecules of squamous and glandular stomach of rats, that were either pretreated with N-methyl-N-nitrosourea (MNU) for different periods or without pretreatment, is studied. The results showed no significant difference in the binding of labeled carcinogen to DNA and RNA of the two portions of stomach. However, a significant increase was noted in the incorporated 3H-MNU in proteins of glandular stomach with or without pretreatment with MNU. From these studies it is implied that carcinogenic effect of MNU may be through interaction with some other molecule(s) rather than with nucleic acids and/or proteins.", "contents": "Binding of labeled N-methyl N-nitrosourea to macromolecules of squamous and glandular stomach of rats. The binding of labeled N-methyl-N-nitrosourea (3H-MNU) to macromolecules of squamous and glandular stomach of rats, that were either pretreated with N-methyl-N-nitrosourea (MNU) for different periods or without pretreatment, is studied. The results showed no significant difference in the binding of labeled carcinogen to DNA and RNA of the two portions of stomach. However, a significant increase was noted in the incorporated 3H-MNU in proteins of glandular stomach with or without pretreatment with MNU. From these studies it is implied that carcinogenic effect of MNU may be through interaction with some other molecule(s) rather than with nucleic acids and/or proteins.", "PMID": 981794}
{"id": "pubmed23n0032_12755", "title": "Detection of nucleic acid-protein complexes by equilibrium ultracentrifugation.", "content": "Formation of complexes between yeast phenylalanine transfer RNA and phenylalanyl aminoacyl-transfer RNA synthetase is shown to occur under equilibrium conditions in the analytical ultracentrifuge. The technique of equilibrium sedimentation should be useful for the detection of transient associations between proteins and nucleic acids when only small amounts of unlabeled, but highly purified, materials are available.", "contents": "Detection of nucleic acid-protein complexes by equilibrium ultracentrifugation. Formation of complexes between yeast phenylalanine transfer RNA and phenylalanyl aminoacyl-transfer RNA synthetase is shown to occur under equilibrium conditions in the analytical ultracentrifuge. The technique of equilibrium sedimentation should be useful for the detection of transient associations between proteins and nucleic acids when only small amounts of unlabeled, but highly purified, materials are available.", "PMID": 981795}
{"id": "pubmed23n0032_12756", "title": "Concentration of inactivated swine influenza virus by molecular filtration for use as a vaccine in gilts before and after breeding.", "content": "Inactivated swine influenza virus was concentrated by molecular filtration for use as a vaccine in gilts before and after breeding. The best serologic response followed the use of two 5 ml doses three weeks apart before breeding. Vaccination with two 5 ml doses two weeks apart after breeding did not result in as marked serologic response. All vaccinated gilts produced detectable hemagglutination inhibition (HI) antibody.", "contents": "Concentration of inactivated swine influenza virus by molecular filtration for use as a vaccine in gilts before and after breeding. Inactivated swine influenza virus was concentrated by molecular filtration for use as a vaccine in gilts before and after breeding. The best serologic response followed the use of two 5 ml doses three weeks apart before breeding. Vaccination with two 5 ml doses two weeks apart after breeding did not result in as marked serologic response. All vaccinated gilts produced detectable hemagglutination inhibition (HI) antibody.", "PMID": 981796}
{"id": "pubmed23n0032_12757", "title": "Lactic dehydrogenase of fetal ear fluids under ambient and high-intensity sound.", "content": "Lactic dehydrogenase (LDH) was determined in the fluids of the inner ear scalae and the middle ear cavity of fetal guinea pigs near term. Scalar dissimilarities in prenatal perilymph and distinctions between fetal and maternal perilymph suggest functional differentiation between the antenatal scalae vestibuli and tympani, elevated glycolytic activity in the prenatal vestibular channel and primarily aerobic processes in fetal perilymph although anaerobic metabolism is also evident. Disparities between fetal middle ear fluid and prenatal perilymph as well as maternal serum indicate that the former medium is probably not directly derived from either of the latter sources. After experimental treatment consisting of the presentation of high-intensity sound to gravid animals, the total LDH activity of the fetal middle ear fluid decreased.", "contents": "Lactic dehydrogenase of fetal ear fluids under ambient and high-intensity sound. Lactic dehydrogenase (LDH) was determined in the fluids of the inner ear scalae and the middle ear cavity of fetal guinea pigs near term. Scalar dissimilarities in prenatal perilymph and distinctions between fetal and maternal perilymph suggest functional differentiation between the antenatal scalae vestibuli and tympani, elevated glycolytic activity in the prenatal vestibular channel and primarily aerobic processes in fetal perilymph although anaerobic metabolism is also evident. Disparities between fetal middle ear fluid and prenatal perilymph as well as maternal serum indicate that the former medium is probably not directly derived from either of the latter sources. After experimental treatment consisting of the presentation of high-intensity sound to gravid animals, the total LDH activity of the fetal middle ear fluid decreased.", "PMID": 981797}
{"id": "pubmed23n0032_12758", "title": "The glycolytic effect of 1-epinephrine and nor-epinephrine on the liver of the chick embryo.", "content": "The glycolytic effect of 1-epinephrine and nor-epinephrine administered in-ovo for two hours on the liver of the chick embryo is reported. 1-epinephrine was more glycolytic than nor-epinephrine throughout the study. The glycolytic effect of 1-epinephrine was dosage-dependent throughout the study while nor-epinephrine brought about dosage-dependent liver-glycogen-depletion only in the nine day embryo.", "contents": "The glycolytic effect of 1-epinephrine and nor-epinephrine on the liver of the chick embryo. The glycolytic effect of 1-epinephrine and nor-epinephrine administered in-ovo for two hours on the liver of the chick embryo is reported. 1-epinephrine was more glycolytic than nor-epinephrine throughout the study. The glycolytic effect of 1-epinephrine was dosage-dependent throughout the study while nor-epinephrine brought about dosage-dependent liver-glycogen-depletion only in the nine day embryo.", "PMID": 981798}
{"id": "pubmed23n0032_12759", "title": "Induction of rat hepatic mixed function monooxygenases by levamisole.", "content": "The effects of levamisole administration (20 mg/kg, i.p. daily for 10 days) on various parameters of hepatic microsomal metabolism in the famale rat were examined. Levamisole pretreatment resulted in significant increases over control values in the metabolism of aminopyrine and aniline in vitro, and in microsomal content of cytochrome P-450 and cytochrome b5.", "contents": "Induction of rat hepatic mixed function monooxygenases by levamisole. The effects of levamisole administration (20 mg/kg, i.p. daily for 10 days) on various parameters of hepatic microsomal metabolism in the famale rat were examined. Levamisole pretreatment resulted in significant increases over control values in the metabolism of aminopyrine and aniline in vitro, and in microsomal content of cytochrome P-450 and cytochrome b5.", "PMID": 981799}
{"id": "pubmed23n0032_12760", "title": "Prolongation of responses in decentralization supersensitivity.", "content": "In chronically decentralized nictitating membranes of cats the responses to norepinephrine and to acetylcholine were markedly prolonged. The increased duration was not directly related to the increased amplitude of the contractions. It is likely to be due to a change of the muscle itself.", "contents": "Prolongation of responses in decentralization supersensitivity. In chronically decentralized nictitating membranes of cats the responses to norepinephrine and to acetylcholine were markedly prolonged. The increased duration was not directly related to the increased amplitude of the contractions. It is likely to be due to a change of the muscle itself.", "PMID": 981800}
{"id": "pubmed23n0032_12761", "title": "Effect of chronic glucagon-administration on the digestive and absorptive function of rat small intestine in vivo.", "content": "Endogeneous hyperglucagonemia is observed in experimental diabetes mellitus and semistarvation, conditions associated with an increased intestinal absorptive function. To examine whether glucagon might exert a similar adaptive response on intestinal digestive-absorptive function like experimental diabetes mellitus the effect of chronic glucagon administration on intestinal transport of 3-0-methyl-D-glucose, water, sodium, potassium, and D-glucose induced transmural potential difference (PD) was examined by an in vivo perfusion technique in rat small intestine. Chronic administration of glucagon (100 mug twice daily) for 5 days resulted in increased absorption of 3-0-methyl-D-glucose, water, sodium and potassium as well as in an increase of D-glucose induced PD. A similar, but more pronounced augmentation of D-glucose induced PD was observed in the jejunum of streptozotocin-diabetic rats. Disaccharidase (maltase, sucrase, trehalase, lactase) and alkaline phosphatase activities were not affected in intestinal mucosa of glucagon-treated rats compared to controls. It cannot be decided from these results whether hyperglucagonemia is responsible for the adaptive intestinal changes observed in experimental diabetes mellitus.", "contents": "Effect of chronic glucagon-administration on the digestive and absorptive function of rat small intestine in vivo. Endogeneous hyperglucagonemia is observed in experimental diabetes mellitus and semistarvation, conditions associated with an increased intestinal absorptive function. To examine whether glucagon might exert a similar adaptive response on intestinal digestive-absorptive function like experimental diabetes mellitus the effect of chronic glucagon administration on intestinal transport of 3-0-methyl-D-glucose, water, sodium, potassium, and D-glucose induced transmural potential difference (PD) was examined by an in vivo perfusion technique in rat small intestine. Chronic administration of glucagon (100 mug twice daily) for 5 days resulted in increased absorption of 3-0-methyl-D-glucose, water, sodium and potassium as well as in an increase of D-glucose induced PD. A similar, but more pronounced augmentation of D-glucose induced PD was observed in the jejunum of streptozotocin-diabetic rats. Disaccharidase (maltase, sucrase, trehalase, lactase) and alkaline phosphatase activities were not affected in intestinal mucosa of glucagon-treated rats compared to controls. It cannot be decided from these results whether hyperglucagonemia is responsible for the adaptive intestinal changes observed in experimental diabetes mellitus.", "PMID": 981801}
{"id": "pubmed23n0032_12762", "title": "Activity of lecithin: cholesterol acyltransferase in different parts of the circulatory system in man.", "content": "In 24 patients without liver disease lipids and LCAT activity were determined in the inferior vena cava, the hepatic vein, the pulmonary vein and in the left ventricle. The total cholesterol, free cholesterol and cholesterol ester concentrations are identical and differences in the LCAT activity cannot be demonstrated.", "contents": "Activity of lecithin: cholesterol acyltransferase in different parts of the circulatory system in man. In 24 patients without liver disease lipids and LCAT activity were determined in the inferior vena cava, the hepatic vein, the pulmonary vein and in the left ventricle. The total cholesterol, free cholesterol and cholesterol ester concentrations are identical and differences in the LCAT activity cannot be demonstrated.", "PMID": 981802}
{"id": "pubmed23n0032_12763", "title": "Excretion of liver antigens in the urine of patients with hepatic diseases.", "content": "Liver antigens were detected in the urine of 4 of 42 patients with various liver diseases. The urine of 25 healthy subjects and patients with diseases not affecting the liver was devoid of antigens in detectable amounts. The presence of hepatic antigens in the urine did not correlate with severity of jaundice and SGOT levels but correlated with parenchymal necrosis and was associtated with a high mortaltiy.", "contents": "Excretion of liver antigens in the urine of patients with hepatic diseases. Liver antigens were detected in the urine of 4 of 42 patients with various liver diseases. The urine of 25 healthy subjects and patients with diseases not affecting the liver was devoid of antigens in detectable amounts. The presence of hepatic antigens in the urine did not correlate with severity of jaundice and SGOT levels but correlated with parenchymal necrosis and was associtated with a high mortaltiy.", "PMID": 981803}
{"id": "pubmed23n0032_12764", "title": "[The value of the thermocouple in the measurement of the gastric mucosal blood-flow. The influence of the occlusion of the celiac artery and prostaglandin E1 on the gastric mucosal blood flow. An experimental study in animals (author's transl)].", "content": "The study has been carried out to ensure the positive evidence of the measurement of the gastric mucosal blood-flow with the aid of the thermocouple (heat-clearance technique). The experiments have shown that the suction pressure of 600 mm mercury column which was used to fix the Thermocouple to the mucosa was indispensable in order to assess the blood-flow in the entire depth of the mucosa. Changes in the mucosal blood-flow are measuured at the same rate in all quadrants of the gastric corpus. The measuring of the blood-flow of a well circumscribed area of the mucosa is therefore representative for the entire corpus. Vasopressin led to a significant reduction of the gastric mucosal blood-flow measured with heat-clearance as well aminopyrine-clearance. There was a linear correlation between the results of both methods. Vasopressin selectively reduces the blood-flow of the gastric mucosa but not of the submucosa, the muscular layer and the serosa. Therefore it seems to be probable that changes in mucosal blood-flow selectively can be measured with the aid of the thermocouple. After previous stimulation with pentagastrin neither mucosal blood-flow nor acid secretion of the stomach were influenced by the occlusion of the celiac artery by 25 %. The occlusion of the celiac artery by 50 % reduced significantly the pentagastrin-stimulated gastric mucosal blood-flow whereas the acid secretion was not influenced. Prostaglandin E1 at a dose rate of 2 mug/kg-h increased significantly arterial and mucosal blood-flow as well as acid secretion of the stomach. In comparison PGE1 administered at a dose rate of 4 mug/kg-h reduced significantly gastric mucosal blood-flow and gastric secretion. PGE1 at a dose rate of 8 mug/kg-h did not produce any significant changes in blood-flow and secretion. The results suggested that the changes of gastric secretion observed with PGE1 were the consequence of primary changes in the gastric mucosal blood-flow.", "contents": "[The value of the thermocouple in the measurement of the gastric mucosal blood-flow. The influence of the occlusion of the celiac artery and prostaglandin E1 on the gastric mucosal blood flow. An experimental study in animals (author's transl)]. The study has been carried out to ensure the positive evidence of the measurement of the gastric mucosal blood-flow with the aid of the thermocouple (heat-clearance technique). The experiments have shown that the suction pressure of 600 mm mercury column which was used to fix the Thermocouple to the mucosa was indispensable in order to assess the blood-flow in the entire depth of the mucosa. Changes in the mucosal blood-flow are measuured at the same rate in all quadrants of the gastric corpus. The measuring of the blood-flow of a well circumscribed area of the mucosa is therefore representative for the entire corpus. Vasopressin led to a significant reduction of the gastric mucosal blood-flow measured with heat-clearance as well aminopyrine-clearance. There was a linear correlation between the results of both methods. Vasopressin selectively reduces the blood-flow of the gastric mucosa but not of the submucosa, the muscular layer and the serosa. Therefore it seems to be probable that changes in mucosal blood-flow selectively can be measured with the aid of the thermocouple. After previous stimulation with pentagastrin neither mucosal blood-flow nor acid secretion of the stomach were influenced by the occlusion of the celiac artery by 25 %. The occlusion of the celiac artery by 50 % reduced significantly the pentagastrin-stimulated gastric mucosal blood-flow whereas the acid secretion was not influenced. Prostaglandin E1 at a dose rate of 2 mug/kg-h increased significantly arterial and mucosal blood-flow as well as acid secretion of the stomach. In comparison PGE1 administered at a dose rate of 4 mug/kg-h reduced significantly gastric mucosal blood-flow and gastric secretion. PGE1 at a dose rate of 8 mug/kg-h did not produce any significant changes in blood-flow and secretion. The results suggested that the changes of gastric secretion observed with PGE1 were the consequence of primary changes in the gastric mucosal blood-flow.", "PMID": 981804}
{"id": "pubmed23n0032_12765", "title": "Metabolism of maltose during surgery in patients with diabetes mellitus under general anesthesia.", "content": "During a surgical operation under general anesthesia, maltose was administered at a rate of 1g/kg BW to 9 diabetic and 18 non-diabetic patients. The disappearance curve of maltose from blood was similar between diabetics and non-diabetics. Blood sugar tended to rise but no significant difference was found between the two groups. Lactic acid and pyruvic acid showed a tendency toward a rise, but the change was non-significant. NEFA and uric acid failed to change. Urinary excretion of maltose was not different between diabetics and non-diabetics. After 120 min., urinary excretion of maltose was similar between the two groups, but excretion as glucose was greater in the diabetic group.", "contents": "Metabolism of maltose during surgery in patients with diabetes mellitus under general anesthesia. During a surgical operation under general anesthesia, maltose was administered at a rate of 1g/kg BW to 9 diabetic and 18 non-diabetic patients. The disappearance curve of maltose from blood was similar between diabetics and non-diabetics. Blood sugar tended to rise but no significant difference was found between the two groups. Lactic acid and pyruvic acid showed a tendency toward a rise, but the change was non-significant. NEFA and uric acid failed to change. Urinary excretion of maltose was not different between diabetics and non-diabetics. After 120 min., urinary excretion of maltose was similar between the two groups, but excretion as glucose was greater in the diabetic group.", "PMID": 981805}
{"id": "pubmed23n0032_12766", "title": "[Changes of triglycerid-fatty acids in healthy volunteers during acute ethanol ingestion with and without blocking peripheral lipolysis (author's transl)].", "content": "This investigation decided to answer the question of the origin of fatty acids for the increased synthesis of triglycerides in acute ethanol-induced hyperlipoproteinemia. Healthy persons ingested 0.5 g of ethanol/kg body weight initially and 0,15 g of ethanol/kg and hour for 12 hours. The fatty acids of plasma triglycerides were determined before and after ingestion of ethanol in persons fasting and nourished isocaloricaly, with and without blocking peripheral lipolysis by nicotinic acid and with addition of glucose. The fasting persons triglycerides fatty acids increased to 165.7 % of the initial value after 12 hours of ethanol ingestion, with a preferential increase in palmitic-, oleic- and stearic acid. When lipolysis in adipose tissue was blocked by 0.5 g of nicotinic adic/hour the triglyceride-fatty acids reached only 116.2% after 12 hours, with a decrease in oleic acid, which is present in adipose tissue to a higher degree than in plasma triglycerides. When nourished isocaloricaly, the enhancement of plasma triglyceride-fatty acids could not be suppressed by nicotinic acid. The changes in concentration and pattern of triglyceride-fatty acids announce that the fatty acids used for increased synthesis of triglycerides in fasting persons come from adipose tissue preferentially. In contrast ethanol-ingested hyperlipoproteinemia during ingestion of a food which cannot be suppressed by nicotinic acid, seems to orginate from fatty acids of the food and for de novo synthesis of fatty acids in the liver.", "contents": "[Changes of triglycerid-fatty acids in healthy volunteers during acute ethanol ingestion with and without blocking peripheral lipolysis (author's transl)]. This investigation decided to answer the question of the origin of fatty acids for the increased synthesis of triglycerides in acute ethanol-induced hyperlipoproteinemia. Healthy persons ingested 0.5 g of ethanol/kg body weight initially and 0,15 g of ethanol/kg and hour for 12 hours. The fatty acids of plasma triglycerides were determined before and after ingestion of ethanol in persons fasting and nourished isocaloricaly, with and without blocking peripheral lipolysis by nicotinic acid and with addition of glucose. The fasting persons triglycerides fatty acids increased to 165.7 % of the initial value after 12 hours of ethanol ingestion, with a preferential increase in palmitic-, oleic- and stearic acid. When lipolysis in adipose tissue was blocked by 0.5 g of nicotinic adic/hour the triglyceride-fatty acids reached only 116.2% after 12 hours, with a decrease in oleic acid, which is present in adipose tissue to a higher degree than in plasma triglycerides. When nourished isocaloricaly, the enhancement of plasma triglyceride-fatty acids could not be suppressed by nicotinic acid. The changes in concentration and pattern of triglyceride-fatty acids announce that the fatty acids used for increased synthesis of triglycerides in fasting persons come from adipose tissue preferentially. In contrast ethanol-ingested hyperlipoproteinemia during ingestion of a food which cannot be suppressed by nicotinic acid, seems to orginate from fatty acids of the food and for de novo synthesis of fatty acids in the liver.", "PMID": 981806}
{"id": "pubmed23n0032_12767", "title": "Rat gastric mucosal oxygen tension, ulcer index, plasma gastrin and glucagon following restraint stress. Influence of vagotomy, splanchnicotomy and exogenous secretin.", "content": "Restraint stress ulcers in rats were developed and procedures evaluated designed at preservation of intact gastric microcirculation (pO2). Neither prior truncal vagotomy, splanchnicotomy nor combined dissection of abdominal autonomic nerves were effective in preventing the stress mediated fall of mucosal pO2 and the rise in plasma glucagon. The ulcer index remained elevated and gastrin essentially was unchanged. Prophylactic injection of increasing doses (1, 2, 4, 8, 16 U/kg secretin maintained microcirculation at pO2-levels subnormal for unstressed animals (Vmax 15.53 mm Hg; Km. 0.99 U/kg), but simultaneously brought about a continuous rise in serum gastrin. Up to 8 U/kg plasma glucagon was higher than in saline control groups reaching a peak value with 2 U/kg when ucler index showed its nadir. Secretin therapy (4, 8 U/kg) markedly improves both mucosal pO2 and ulcer index. It is suggested that breakdown of gastric microcirculation may not be solely responsible for stress ulcer development.", "contents": "Rat gastric mucosal oxygen tension, ulcer index, plasma gastrin and glucagon following restraint stress. Influence of vagotomy, splanchnicotomy and exogenous secretin. Restraint stress ulcers in rats were developed and procedures evaluated designed at preservation of intact gastric microcirculation (pO2). Neither prior truncal vagotomy, splanchnicotomy nor combined dissection of abdominal autonomic nerves were effective in preventing the stress mediated fall of mucosal pO2 and the rise in plasma glucagon. The ulcer index remained elevated and gastrin essentially was unchanged. Prophylactic injection of increasing doses (1, 2, 4, 8, 16 U/kg secretin maintained microcirculation at pO2-levels subnormal for unstressed animals (Vmax 15.53 mm Hg; Km. 0.99 U/kg), but simultaneously brought about a continuous rise in serum gastrin. Up to 8 U/kg plasma glucagon was higher than in saline control groups reaching a peak value with 2 U/kg when ucler index showed its nadir. Secretin therapy (4, 8 U/kg) markedly improves both mucosal pO2 and ulcer index. It is suggested that breakdown of gastric microcirculation may not be solely responsible for stress ulcer development.", "PMID": 981807}
{"id": "pubmed23n0032_12768", "title": "[Transformation of monocytes into fibroblasts in wound healing (author's transl)].", "content": "Recent investigations of HELPAP and CREMER 1972 seem to confirm the supposition of COHNHEIM 1867 that monocytes of the peripheral blood are transformed into firbroblasts in healing wounds. The view of VIRCHOW 1871, MARCHAND 1901 and ASCHOFF 1924 that the fibroblasts in healing wounds are of local origin seems to be supported by the invesigation on parabiotic inbred rats of ROSS et al. 1970. We thought that irradiating the whole body of rabbits could be of value in resolving these apparent contradictions about the origin of fibroblasts in healing wounds.", "contents": "[Transformation of monocytes into fibroblasts in wound healing (author's transl)]. Recent investigations of HELPAP and CREMER 1972 seem to confirm the supposition of COHNHEIM 1867 that monocytes of the peripheral blood are transformed into firbroblasts in healing wounds. The view of VIRCHOW 1871, MARCHAND 1901 and ASCHOFF 1924 that the fibroblasts in healing wounds are of local origin seems to be supported by the invesigation on parabiotic inbred rats of ROSS et al. 1970. We thought that irradiating the whole body of rabbits could be of value in resolving these apparent contradictions about the origin of fibroblasts in healing wounds.", "PMID": 981808}
{"id": "pubmed23n0032_12769", "title": "Induction of nephrotoxic nephritis with purified antibody: preparation, properties and assay of antibody.", "content": "Antibody to rabbit glomerular basement membrane was separated from whole antiserum by adsorption onto and elution from rabbit glomeruli in vitro. The physico-chemical and biological properties were investigated. The preparation had a high specific activity for the GBM and induced glomerulonephritis on injection into rabbits. About 10% of the antibody localised in the kidneys of rabbits and the total in vitro and in vivo tissue binding capacity were comparable. Based on these results a simple in vitro assay for measuring anti-GBM antibody is described.", "contents": "Induction of nephrotoxic nephritis with purified antibody: preparation, properties and assay of antibody. Antibody to rabbit glomerular basement membrane was separated from whole antiserum by adsorption onto and elution from rabbit glomeruli in vitro. The physico-chemical and biological properties were investigated. The preparation had a high specific activity for the GBM and induced glomerulonephritis on injection into rabbits. About 10% of the antibody localised in the kidneys of rabbits and the total in vitro and in vivo tissue binding capacity were comparable. Based on these results a simple in vitro assay for measuring anti-GBM antibody is described.", "PMID": 981809}
{"id": "pubmed23n0032_12770", "title": "[On the mechanisms of biliary excretion of the biliary contrast medium ioglycamide in the mini-pig with special regard of the importance of the bile flow (author's transl)].", "content": "16 experiments were performed in order to investigate the mechanisms of biliary excretion of Ioglycamide in the mini-pig. The results confirm the mechanisms known from other species for this experimental animal. Additionally, however, they demonstrate a significant linear correlation between the spontaneous bile flow and the biliary transport-maximum of Ioglycamide. It is concluded that this correlation is due to the fact that the bile flow as well as the transport-maximum represent independent parameters of the excretory capacity of the liver cells. This conclusion is supported by further experiments that were unable to show an improvement of the biliary transport-maximum of Ioglycamide by stimulation of the bile flow during infusions of bile acids and secretin. These results prove the impossibility to enhance the biliary excretion of Ioglycamide by stimulation of bile flow. This, however, indicates that it is possible to dilute or concentrate the biliary Ioglycamide concentration by induced variations of bile flow, a fact that might become important to improve the efficiency of i.v. cholegraphy.", "contents": "[On the mechanisms of biliary excretion of the biliary contrast medium ioglycamide in the mini-pig with special regard of the importance of the bile flow (author's transl)]. 16 experiments were performed in order to investigate the mechanisms of biliary excretion of Ioglycamide in the mini-pig. The results confirm the mechanisms known from other species for this experimental animal. Additionally, however, they demonstrate a significant linear correlation between the spontaneous bile flow and the biliary transport-maximum of Ioglycamide. It is concluded that this correlation is due to the fact that the bile flow as well as the transport-maximum represent independent parameters of the excretory capacity of the liver cells. This conclusion is supported by further experiments that were unable to show an improvement of the biliary transport-maximum of Ioglycamide by stimulation of the bile flow during infusions of bile acids and secretin. These results prove the impossibility to enhance the biliary excretion of Ioglycamide by stimulation of bile flow. This, however, indicates that it is possible to dilute or concentrate the biliary Ioglycamide concentration by induced variations of bile flow, a fact that might become important to improve the efficiency of i.v. cholegraphy.", "PMID": 981810}
{"id": "pubmed23n0032_12771", "title": "Inactivation of contraction as a determinant of the length-active tension relation in heart muscle of the cat.", "content": "In isometrically contracting isolated cat papillary muscles, the mechanism determining the reduction in active tension (P) at lengths (L) below the optimal length (Lmax) for maximal tension development (Pmax) was studied. Increasing [Ca2+] from 1.25 to 5 mM, paired electrical stimulation and caffeine augmented P-O/max by 77,61 and 23% respectively, as well as the tension developed at any given length on the ascending limb of the length-active tension relation. To see whether below Lmax the degree of activation is dependent on length, these length-active tension relations were expressed as P/Pmax relative to L/Lmax where Pmax and Lmax in each inotropic state was 100%. These normalized length-active tension relations were not altered by different [Ca2+]o, but shifted upward and to the left by caffeine and paired stimulation. The effects of elevating [Ca2+] on the length-active tension relation suggests that restoring forces and fixed internal loads are not an important factor in decreasing active tension at short lengths. The effects of caffeine and paired stimulation support the view that in cardiac muscle inactivitation of contraction is a major factor determining the fall in active tension at decreasing length.", "contents": "Inactivation of contraction as a determinant of the length-active tension relation in heart muscle of the cat. In isometrically contracting isolated cat papillary muscles, the mechanism determining the reduction in active tension (P) at lengths (L) below the optimal length (Lmax) for maximal tension development (Pmax) was studied. Increasing [Ca2+] from 1.25 to 5 mM, paired electrical stimulation and caffeine augmented P-O/max by 77,61 and 23% respectively, as well as the tension developed at any given length on the ascending limb of the length-active tension relation. To see whether below Lmax the degree of activation is dependent on length, these length-active tension relations were expressed as P/Pmax relative to L/Lmax where Pmax and Lmax in each inotropic state was 100%. These normalized length-active tension relations were not altered by different [Ca2+]o, but shifted upward and to the left by caffeine and paired stimulation. The effects of elevating [Ca2+] on the length-active tension relation suggests that restoring forces and fixed internal loads are not an important factor in decreasing active tension at short lengths. The effects of caffeine and paired stimulation support the view that in cardiac muscle inactivitation of contraction is a major factor determining the fall in active tension at decreasing length.", "PMID": 981811}
{"id": "pubmed23n0032_12772", "title": "[Muscle metabolism of glucose, lactate, free fatty acids, and glycerol at rest and during electrically stimulated exercise in the perfused rat hind limb (author's transl)].", "content": "The influence of insulin on muscle metabolism at rest and during electrically stimulated exercise was examined in the perfused rat hind limb. Basal glucose uptake of muscle tissue was enhanced threefold both by muscle contraction an by addition of physiological amounts of insulin. A further increase in glucose uptake was seen, when muscle was exercised in the presence of insulin. Lactate production rose 7 - 8fold by contractions. Insulin had no additional effect on lactate production during exercise. The work-induced lactate output exceeded glucose uptake, corresponding to a significantly reduced glycogen content after exercise. Basal lipolysis was not stimulated by muscle contractions. At rest the addition of both corticosterone and thyroxine did not increase the basal lipolysis. Yet, glycerol release rose twofold by the addition of serum to the perfusion medium. It is assumed that in the perfused rat hind limb glycogenolysis will promptly be stimulated by muscle contractions, whereas lipolysis can not be enhanced by exercise alone.", "contents": "[Muscle metabolism of glucose, lactate, free fatty acids, and glycerol at rest and during electrically stimulated exercise in the perfused rat hind limb (author's transl)]. The influence of insulin on muscle metabolism at rest and during electrically stimulated exercise was examined in the perfused rat hind limb. Basal glucose uptake of muscle tissue was enhanced threefold both by muscle contraction an by addition of physiological amounts of insulin. A further increase in glucose uptake was seen, when muscle was exercised in the presence of insulin. Lactate production rose 7 - 8fold by contractions. Insulin had no additional effect on lactate production during exercise. The work-induced lactate output exceeded glucose uptake, corresponding to a significantly reduced glycogen content after exercise. Basal lipolysis was not stimulated by muscle contractions. At rest the addition of both corticosterone and thyroxine did not increase the basal lipolysis. Yet, glycerol release rose twofold by the addition of serum to the perfusion medium. It is assumed that in the perfused rat hind limb glycogenolysis will promptly be stimulated by muscle contractions, whereas lipolysis can not be enhanced by exercise alone.", "PMID": 981812}
{"id": "pubmed23n0032_12773", "title": "[The effect of dopamin on the microcirculation on the pancreas in hemorrhagic-shock (author's transl)].", "content": "The influence of Dopamine on microcirculation during hemorrhagic shock was investigated by means of the pancreas chamber technique (HEISIG, 1967). Parameter of microcirculation was the corpuscular flow velocity measured with the \"flying spot\" method. Simultaneously arterial and venous blood pressure, heart rate, blood gases and pH-status were registered. While a Dopamine-dosage of 5 mug/kg/min did not markedly effect perfusion of the pancreas, application of 10 as well as 20 mug/kg/min Dopamine caused a significant improvement of microcirculation. There was no difference between 10 and 20 mug/kg/min Dopamine. The increase of capillary perfusion can certainly not be explained by the small rise of blood pressure that was found under treatment with Dopamine. The results support the statement of the existence of Dopamine-specific receptors in pancreatic vessels. Compared to a group of animals treated with 5 mug/kg/min Dopamine and an untreated control group, survival rate of animals treated with 10 as well as 20 mug/kg/min Dopamine was significantly increased.", "contents": "[The effect of dopamin on the microcirculation on the pancreas in hemorrhagic-shock (author's transl)]. The influence of Dopamine on microcirculation during hemorrhagic shock was investigated by means of the pancreas chamber technique (HEISIG, 1967). Parameter of microcirculation was the corpuscular flow velocity measured with the \"flying spot\" method. Simultaneously arterial and venous blood pressure, heart rate, blood gases and pH-status were registered. While a Dopamine-dosage of 5 mug/kg/min did not markedly effect perfusion of the pancreas, application of 10 as well as 20 mug/kg/min Dopamine caused a significant improvement of microcirculation. There was no difference between 10 and 20 mug/kg/min Dopamine. The increase of capillary perfusion can certainly not be explained by the small rise of blood pressure that was found under treatment with Dopamine. The results support the statement of the existence of Dopamine-specific receptors in pancreatic vessels. Compared to a group of animals treated with 5 mug/kg/min Dopamine and an untreated control group, survival rate of animals treated with 10 as well as 20 mug/kg/min Dopamine was significantly increased.", "PMID": 981813}
{"id": "pubmed23n0032_12774", "title": "The consequences of ischaemia after mechanical obstruction of the dog ileum.", "content": "A mechanical obstruction was produced in the dog ileum, and four days later, loop of intestine above and below the occlusion were subjected to one hour's ischaemia. This led to widespread morphological and functional damage of the epithelium, indistinguishable from the response of the normal intestine. The recovery of the mucosa of both loops after the ischaemia followed exactly the same pattern as when there was no occlusion superimposed: The transport capacity for phenylalanine in vitro was entirely restored, whereas that of beta-methyl-glucoside, together with oxygen consumption and lactate production, remained depressed; morphometric examination revealed that the recuperating mucosa had smaller villi, shorter crypts and smaller epithelial cells than the contiguous untouched tissue. These results suggest that the regenerative potential of the crypts could not be obliterated by one hour's ischaemia, either in the presence of a noxious obstruction fluid above the occlusion, or in the atrophic mucosa below it.", "contents": "The consequences of ischaemia after mechanical obstruction of the dog ileum. A mechanical obstruction was produced in the dog ileum, and four days later, loop of intestine above and below the occlusion were subjected to one hour's ischaemia. This led to widespread morphological and functional damage of the epithelium, indistinguishable from the response of the normal intestine. The recovery of the mucosa of both loops after the ischaemia followed exactly the same pattern as when there was no occlusion superimposed: The transport capacity for phenylalanine in vitro was entirely restored, whereas that of beta-methyl-glucoside, together with oxygen consumption and lactate production, remained depressed; morphometric examination revealed that the recuperating mucosa had smaller villi, shorter crypts and smaller epithelial cells than the contiguous untouched tissue. These results suggest that the regenerative potential of the crypts could not be obliterated by one hour's ischaemia, either in the presence of a noxious obstruction fluid above the occlusion, or in the atrophic mucosa below it.", "PMID": 981814}
{"id": "pubmed23n0032_12775", "title": "[Temperature dependence of metabolic processes in perfused rat liver (author's transl)].", "content": "The temperature dependence of various metabolic processes (glycolysis, fructolysis, glycogenolysis, ketogenesis, CO2-production, oxidative decarboxylation of pyruvate and gluconeogenesis) was studied in perfused rat livers. The processes measured differed with respect to their temperature dependence. Moreover, the sensitivity towards changes in temperature differed depending on the temperature range. Q10 values were found between 1,7 and 25 corresponding to values for activation energies between 38 and 216 kJoule/Mol.", "contents": "[Temperature dependence of metabolic processes in perfused rat liver (author's transl)]. The temperature dependence of various metabolic processes (glycolysis, fructolysis, glycogenolysis, ketogenesis, CO2-production, oxidative decarboxylation of pyruvate and gluconeogenesis) was studied in perfused rat livers. The processes measured differed with respect to their temperature dependence. Moreover, the sensitivity towards changes in temperature differed depending on the temperature range. Q10 values were found between 1,7 and 25 corresponding to values for activation energies between 38 and 216 kJoule/Mol.", "PMID": 981815}
{"id": "pubmed23n0032_12776", "title": "Effects of an i. v. injection of ethyl palmitate emulsion on nucleic acids and fat content of the liver.", "content": "In experiments with rats \"chemically\" splenectomized by an i.v. ethyl palmitate injection the possible side effects on the liver were followed. In the liver proteins, DNA, RNA, esterified fatty acids, and in blood serum transaminases were assessed. In all examinations the variances were only temporary and could be characterized as manifestations of a metabolic load rather than actual liver damage.", "contents": "Effects of an i. v. injection of ethyl palmitate emulsion on nucleic acids and fat content of the liver. In experiments with rats \"chemically\" splenectomized by an i.v. ethyl palmitate injection the possible side effects on the liver were followed. In the liver proteins, DNA, RNA, esterified fatty acids, and in blood serum transaminases were assessed. In all examinations the variances were only temporary and could be characterized as manifestations of a metabolic load rather than actual liver damage.", "PMID": 981816}
{"id": "pubmed23n0032_12777", "title": "[Reaction of the cardio-respiratory-system following acetylcholine inhalation and the dependency from the body position on anaesthetised dogs (author's transl)].", "content": "The bronchomotoric reaction following ACH-aerosol in low concentrations is dependend on the position of the body on dogs. Up till one maximal reaction the increase of the airway resistance is always stronger in the lateral position. Nevertheless the breathing minute volume is constant. Concentration reaction curves will be shown. Arterial blood gases, heart rate, and pressure in the arteria femoralis were also measured. The different strongness of the reaction following ACH-inhalation will be caused by the predominantely unilateral ventilation of the animals in the lateral position.", "contents": "[Reaction of the cardio-respiratory-system following acetylcholine inhalation and the dependency from the body position on anaesthetised dogs (author's transl)]. The bronchomotoric reaction following ACH-aerosol in low concentrations is dependend on the position of the body on dogs. Up till one maximal reaction the increase of the airway resistance is always stronger in the lateral position. Nevertheless the breathing minute volume is constant. Concentration reaction curves will be shown. Arterial blood gases, heart rate, and pressure in the arteria femoralis were also measured. The different strongness of the reaction following ACH-inhalation will be caused by the predominantely unilateral ventilation of the animals in the lateral position.", "PMID": 981817}
{"id": "pubmed23n0032_12778", "title": "[Some mechanical properties of the pig aorta (author's transl)].", "content": "In a machine for tensile tests the ultimate strength, stress and strain of the aorta ascendens of 47 female pigs (German landrace) at three different regions of the vessel wall were determined. The animals were fattened under identical conditions in a progeny testing station and slaughtered at the same living weight (102 +/- 2 kg). Weak regressions of body weight and age on parameters measured were nevertheless accounted for. The average elastin content of the aorta ascendens diminished with advancing age but was not significantly correlated to biomechanical properties of the three single samples. Maximum load, stress and modulus of vessel wall segment I (stamped out nearer to the heart) was greater than in sample II. These circumferential segments however exhibited highly stronger biomechanical resistance than sample III, which was stamped longitudinally. In samples I and II there was a negative correlation between thickness of the wall and ultimate tensile stress (N/mm2). Freezing of the material prior to test at -18degrees C for one week resulted in a significant rise in ultimate tensile strength and stress. The meaning of these findings for comparative angiology is discussed.", "contents": "[Some mechanical properties of the pig aorta (author's transl)]. In a machine for tensile tests the ultimate strength, stress and strain of the aorta ascendens of 47 female pigs (German landrace) at three different regions of the vessel wall were determined. The animals were fattened under identical conditions in a progeny testing station and slaughtered at the same living weight (102 +/- 2 kg). Weak regressions of body weight and age on parameters measured were nevertheless accounted for. The average elastin content of the aorta ascendens diminished with advancing age but was not significantly correlated to biomechanical properties of the three single samples. Maximum load, stress and modulus of vessel wall segment I (stamped out nearer to the heart) was greater than in sample II. These circumferential segments however exhibited highly stronger biomechanical resistance than sample III, which was stamped longitudinally. In samples I and II there was a negative correlation between thickness of the wall and ultimate tensile stress (N/mm2). Freezing of the material prior to test at -18degrees C for one week resulted in a significant rise in ultimate tensile strength and stress. The meaning of these findings for comparative angiology is discussed.", "PMID": 981818}
{"id": "pubmed23n0032_12779", "title": "Thrombus formation and microaggregate removal during prolonged extracorporeal circulation using a capillary membrane oxygenator. A scanning electron microscope study.", "content": "Despite adequate heparinization, thrombus formation occurs at the blood/foreign material interface. Depending on the part of the device, the material and the blood flow, the amount and the components of thrombi vary greatly. This is not dangerous by itself but clots can be expelled downstream and damage the lungs, brain, kidney and retina. Interposition of a filter may prevent risks of embolization. The present paper reports the scanning electron microscope study carried out on the Awad oxygenator after use during prolonged extracorporeal circulation on 13 dogs. No filter has been inserted in the circuit for the first six experiments. A screen filter (4) or a polyurethane foam filter (3) was employed in the following ones.", "contents": "Thrombus formation and microaggregate removal during prolonged extracorporeal circulation using a capillary membrane oxygenator. A scanning electron microscope study. Despite adequate heparinization, thrombus formation occurs at the blood/foreign material interface. Depending on the part of the device, the material and the blood flow, the amount and the components of thrombi vary greatly. This is not dangerous by itself but clots can be expelled downstream and damage the lungs, brain, kidney and retina. Interposition of a filter may prevent risks of embolization. The present paper reports the scanning electron microscope study carried out on the Awad oxygenator after use during prolonged extracorporeal circulation on 13 dogs. No filter has been inserted in the circuit for the first six experiments. A screen filter (4) or a polyurethane foam filter (3) was employed in the following ones.", "PMID": 981819}
{"id": "pubmed23n0032_12780", "title": "Estimation of contact time and diffusing capacity for oxygen in the chorioallantoic vascular plexus.", "content": "The contact time of erythrocyte in the chorioallantioc capillaries of chicken embryos was estimated by referring to the oxygenation rate measured with a microphotometer. The chorioallantioc membrane was excised from an incubated egg and the SO2 of blood in it's capillary was changed by varying the gas composition around the membrane from the venous blood PO2 level to the air space gas. The oxygenation time (te) required to attain the level of arterialized blood SO2 was measured as the contact time (tc) in the capillaries, which resulted in 0.87, 0.74, 0.57, 0.49 and 0.36 sec for 10, 12, 14, 16 and 18 days of incubation, respectively. The obtained te value coincided with the contact time calculated from the CO diffusing capacity referring to the reaction rate of CO with oxygenated erythrocyte. Using the te value, the diffusing capacity for O2 in the chorioallantoic capillary was calculated; 1.1, 2.2, 4.4, 6.0 and 6.6 x 10(-3) ml-min-1 - mm Hg-1 for the same incubation days as above. The capillary blood volume (Vc) was also estimated, which increased from about 16 to 35 mul during development from 10 to 18 days. The values of DO2 and Vc converted per kg weight of embryo at the days near hatching were similar to those per kg body weight estimated in human lung.", "contents": "Estimation of contact time and diffusing capacity for oxygen in the chorioallantoic vascular plexus. The contact time of erythrocyte in the chorioallantioc capillaries of chicken embryos was estimated by referring to the oxygenation rate measured with a microphotometer. The chorioallantioc membrane was excised from an incubated egg and the SO2 of blood in it's capillary was changed by varying the gas composition around the membrane from the venous blood PO2 level to the air space gas. The oxygenation time (te) required to attain the level of arterialized blood SO2 was measured as the contact time (tc) in the capillaries, which resulted in 0.87, 0.74, 0.57, 0.49 and 0.36 sec for 10, 12, 14, 16 and 18 days of incubation, respectively. The obtained te value coincided with the contact time calculated from the CO diffusing capacity referring to the reaction rate of CO with oxygenated erythrocyte. Using the te value, the diffusing capacity for O2 in the chorioallantoic capillary was calculated; 1.1, 2.2, 4.4, 6.0 and 6.6 x 10(-3) ml-min-1 - mm Hg-1 for the same incubation days as above. The capillary blood volume (Vc) was also estimated, which increased from about 16 to 35 mul during development from 10 to 18 days. The values of DO2 and Vc converted per kg weight of embryo at the days near hatching were similar to those per kg body weight estimated in human lung.", "PMID": 981824}
{"id": "pubmed23n0032_12781", "title": "Cardiac output determination by simple one-step rebreathing technique.", "content": "We have developed a rebreathing technique for measuring cardiac output in resting or exercising subjects. The data needed are the subject's CO2 dissociation curve, the initial volume and CO2 fraction of the rebreathing bag, and a record of CO2 at the mouth during the maneuver. From these one can obtain all the values required to solve the Fick equation. The combined error due to inaccuracy in reading the tracings and to the simplifying assumptions was found to be small (mean = 0.5%, SD ;.5%). Cardiac output values determined with this technique in normal subjects were on the average 2% higher than those obtained simultaneously with an acetylene rebreathing method (n = 49, SD = 11%). Among the advantages of the technique are that it requires analysis of a single gas, takes less than thirty seconds per determination, allows one to obtain repeated measurements at rapid intervals, is not affected by the ability of lung tissue to store CO2, and eliminates many of the assumptions usually made in non-invasive measurements of cardiac output.", "contents": "Cardiac output determination by simple one-step rebreathing technique. We have developed a rebreathing technique for measuring cardiac output in resting or exercising subjects. The data needed are the subject's CO2 dissociation curve, the initial volume and CO2 fraction of the rebreathing bag, and a record of CO2 at the mouth during the maneuver. From these one can obtain all the values required to solve the Fick equation. The combined error due to inaccuracy in reading the tracings and to the simplifying assumptions was found to be small (mean = 0.5%, SD ;.5%). Cardiac output values determined with this technique in normal subjects were on the average 2% higher than those obtained simultaneously with an acetylene rebreathing method (n = 49, SD = 11%). Among the advantages of the technique are that it requires analysis of a single gas, takes less than thirty seconds per determination, allows one to obtain repeated measurements at rapid intervals, is not affected by the ability of lung tissue to store CO2, and eliminates many of the assumptions usually made in non-invasive measurements of cardiac output.", "PMID": 981825}
{"id": "pubmed23n0032_12782", "title": "Stress at the pleural surface.", "content": "The transmission of forces between the visceral and parietal pleural is modelled as being mediated by two distinct pathways: (a) a contact pressure (Pcon) acting on an area of contact (Acon) and (2) a pleural liquid pressure (Pliq) acting on the area of liquid anfractuosities (Aliq). Summation of the forces yields a new equation relating pleural surface pressure (Ppl) to Pcon, Acon, Pliq, and Aliq. A complete solution of the equation is restricted by present limitations on measurement technology. However, assuming that the volume of pleural liquid is constant during tidal breathing, analysis of the model explains the findings that tidal swings in Pliq (deltaPliq) are sometimes greater than tidal swings in Ppl (deltaPpl). It also predicts that the influence of a change of lung elastic recoil on Pliq may be different in magnitude than its effect on Ppl. Therefore deltaPliq is not necessarily equivalent to deltaPpl.", "contents": "Stress at the pleural surface. The transmission of forces between the visceral and parietal pleural is modelled as being mediated by two distinct pathways: (a) a contact pressure (Pcon) acting on an area of contact (Acon) and (2) a pleural liquid pressure (Pliq) acting on the area of liquid anfractuosities (Aliq). Summation of the forces yields a new equation relating pleural surface pressure (Ppl) to Pcon, Acon, Pliq, and Aliq. A complete solution of the equation is restricted by present limitations on measurement technology. However, assuming that the volume of pleural liquid is constant during tidal breathing, analysis of the model explains the findings that tidal swings in Pliq (deltaPliq) are sometimes greater than tidal swings in Ppl (deltaPpl). It also predicts that the influence of a change of lung elastic recoil on Pliq may be different in magnitude than its effect on Ppl. Therefore deltaPliq is not necessarily equivalent to deltaPpl.", "PMID": 981826}
{"id": "pubmed23n0032_12783", "title": "'Exponential peeling' of ventilatory transients following inhalation of 5, 6 and 7% CO2.", "content": "The 'exponential peeling' technique has been applied to minute ventilation and tidal volume transients occurring after the abrupt removal of 7, 6 and 5% CO2 in inspired air. These transients, in many cases, were found to be composed of three exponential components, each contributing to the total ventilatory response and each having individual time responses. Gelfand and Lambertsen (1973) have attributed these components to the peripheral chemoreceptors as a group and to two central chemoreceptors. Statistical analysis to determine the constancy of the contribution of the three components over the range of CO2 values studied showed that, although the values for each at the different stimulus levels were not significantly different, the great subject-to-subject variation in the data precluded a firm conclusion about the constancy of the components. Because of a number of considerations it was concluded that exponential peeling of respiratory transients following abrupt removal of CO2 inhalation is not a satisfactory way to approach the problem of the numbers, relative contributions and time responses of the various receptor groups comprising the respiratory controller.", "contents": "'Exponential peeling' of ventilatory transients following inhalation of 5, 6 and 7% CO2. The 'exponential peeling' technique has been applied to minute ventilation and tidal volume transients occurring after the abrupt removal of 7, 6 and 5% CO2 in inspired air. These transients, in many cases, were found to be composed of three exponential components, each contributing to the total ventilatory response and each having individual time responses. Gelfand and Lambertsen (1973) have attributed these components to the peripheral chemoreceptors as a group and to two central chemoreceptors. Statistical analysis to determine the constancy of the contribution of the three components over the range of CO2 values studied showed that, although the values for each at the different stimulus levels were not significantly different, the great subject-to-subject variation in the data precluded a firm conclusion about the constancy of the components. Because of a number of considerations it was concluded that exponential peeling of respiratory transients following abrupt removal of CO2 inhalation is not a satisfactory way to approach the problem of the numbers, relative contributions and time responses of the various receptor groups comprising the respiratory controller.", "PMID": 981827}
{"id": "pubmed23n0032_12784", "title": "[Anatomoclinical and angiographic study of a case of disseminated thromboangiosis with predominant cerebral manifestations].", "content": "The authors describe the case of a patient of 22 with hypertension and livedo reticularis who, after presenting with a left brachial monoparesis became progressively demented over a period of five years and died at the age of 27 from a cerebro-meningeal haemorrhage. Angiographically, diffuse distal obliteration of the cerebral arteries was seen with deep networks of the moyamoya type involving the lenticulostriate arteries; similar changes were present in the upper left limb. Histopathological investigations showed obliterative thromboangiitis affecting not only the cerebrum, the brain stem and the cerebellum, but also the viscera. Analysis of this case and consideration of the theoretical possibilities leads the authors to urge that thromboangiosis together with its cerebral manifestations should be considered a nosological entity.", "contents": "[Anatomoclinical and angiographic study of a case of disseminated thromboangiosis with predominant cerebral manifestations]. The authors describe the case of a patient of 22 with hypertension and livedo reticularis who, after presenting with a left brachial monoparesis became progressively demented over a period of five years and died at the age of 27 from a cerebro-meningeal haemorrhage. Angiographically, diffuse distal obliteration of the cerebral arteries was seen with deep networks of the moyamoya type involving the lenticulostriate arteries; similar changes were present in the upper left limb. Histopathological investigations showed obliterative thromboangiitis affecting not only the cerebrum, the brain stem and the cerebellum, but also the viscera. Analysis of this case and consideration of the theoretical possibilities leads the authors to urge that thromboangiosis together with its cerebral manifestations should be considered a nosological entity.", "PMID": 981858}
{"id": "pubmed23n0032_12785", "title": "[Nature of the curves of the cranial vault and their relationship with various brain structures].", "content": "A radiometric investigation for various angles of two dry craniums and of the encephalographic or cerebral arteriographic pictures of 250 subjects has revealed the following facts: The architectural lines of the dome of the skull found by orthogonal projection of the profile, from the front, and in vertical projection, are hyperbolic spirals starting from the base of the skull and converging on the sphenoid. These lines join up in the parietal region forming a hyperbole. The frontal spiral is a simple hyperbolic spiral. The temporal spiral is of the lituus type. The axes of these lines converge in a frontal plane on the epiphysis. The axis of the parietal hyperbole on a projection of the profile passes through the epiphysis and is followed by the parietal artery. Central cranio-encephalic formations follow the same spiral path. The contour of the upper edge of the cerebellum is a lituus spiral. The outline of the lower part of the cerebellum is a simple hyperbolic spiral. The right side of the polar origins of a spiral plotted in the plane of the insula coincides with the right side of the opercula. It can always be plotted, in theory, and forms an angle of 22 degrees 30' with the right side of the median frontal spiral. It cuts the axis of the parietal hyperbole at the apex of the insular triange. The bissector of the angle formed by the two right sides of the polar origins bissects the axis of the parietal hyperbole at a point corresponding to the epiphysis. The temporal hyperbolic lituus spiral has its focal point under the anterior, clinoid, at the junction of the segments CII and CIII of the carotid siphon the curve of which follows the convolutions of the temporal spiral. The right side of the polar origins of this curve indicates the mean direction of the posterior cerebral. The contours of the corpus callosum are homothetic with those of the dome of the skull as far as the axis of the parietal hyperbole. The frontal and parietal curves converge according to four types in the main Pacchionian granulations. The sections of the frontal spiral are often marked by bone abnormalities on the internal table. This analytic test is invariably valid whatever the constants of the spirals or the eccentricities of the hyperboles. Combinations between spirals with different constants and hyperboles of various eccentricities amount to very many types which call for separate study.", "contents": "[Nature of the curves of the cranial vault and their relationship with various brain structures]. A radiometric investigation for various angles of two dry craniums and of the encephalographic or cerebral arteriographic pictures of 250 subjects has revealed the following facts: The architectural lines of the dome of the skull found by orthogonal projection of the profile, from the front, and in vertical projection, are hyperbolic spirals starting from the base of the skull and converging on the sphenoid. These lines join up in the parietal region forming a hyperbole. The frontal spiral is a simple hyperbolic spiral. The temporal spiral is of the lituus type. The axes of these lines converge in a frontal plane on the epiphysis. The axis of the parietal hyperbole on a projection of the profile passes through the epiphysis and is followed by the parietal artery. Central cranio-encephalic formations follow the same spiral path. The contour of the upper edge of the cerebellum is a lituus spiral. The outline of the lower part of the cerebellum is a simple hyperbolic spiral. The right side of the polar origins of a spiral plotted in the plane of the insula coincides with the right side of the opercula. It can always be plotted, in theory, and forms an angle of 22 degrees 30' with the right side of the median frontal spiral. It cuts the axis of the parietal hyperbole at the apex of the insular triange. The bissector of the angle formed by the two right sides of the polar origins bissects the axis of the parietal hyperbole at a point corresponding to the epiphysis. The temporal hyperbolic lituus spiral has its focal point under the anterior, clinoid, at the junction of the segments CII and CIII of the carotid siphon the curve of which follows the convolutions of the temporal spiral. The right side of the polar origins of this curve indicates the mean direction of the posterior cerebral. The contours of the corpus callosum are homothetic with those of the dome of the skull as far as the axis of the parietal hyperbole. The frontal and parietal curves converge according to four types in the main Pacchionian granulations. The sections of the frontal spiral are often marked by bone abnormalities on the internal table. This analytic test is invariably valid whatever the constants of the spirals or the eccentricities of the hyperboles. Combinations between spirals with different constants and hyperboles of various eccentricities amount to very many types which call for separate study.", "PMID": 981859}
{"id": "pubmed23n0032_12786", "title": "[Cerebral cystercosis. 3. cases observed in the Paris area in immigrant workers].", "content": "The three cases described involve two Portugese patients and a Spaniard working in the Paris are who had presented with epilepsy, being focal in two. Diagnosis was made in two cases as a result of the existence of muscular and sub-cutaneous calcifications; in one case because of eosinophilia of the cerebro-spinal fluid. The clinical and therapeutic features of cerebral cysticercosis are recalled briefly in connection with these cases.", "contents": "[Cerebral cystercosis. 3. cases observed in the Paris area in immigrant workers]. The three cases described involve two Portugese patients and a Spaniard working in the Paris are who had presented with epilepsy, being focal in two. Diagnosis was made in two cases as a result of the existence of muscular and sub-cutaneous calcifications; in one case because of eosinophilia of the cerebro-spinal fluid. The clinical and therapeutic features of cerebral cysticercosis are recalled briefly in connection with these cases.", "PMID": 981860}
{"id": "pubmed23n0032_12787", "title": "[Amyotrophic lateral sclerosis occuring befor the age of 40 years. Remarks apropos of 25 cases].", "content": "Twenty-five cases of amyotrophic lateral sclerosis occurring before the age of 40, collected between 1963 and 1973 in the Neurological Hospital, Lyons, have prompted the authors to make the following observations. -The incidence of disease seems to have been on the increase during this period both in absolute terms and relatively speaking (relative to the total number of patients hospitalized and to the total number of patients hospitalized for amyotrophic lateral sclerosis after the age of forty). -The 25 cases involved mostly women--the opposite of what was found in cases of amyotrophic lateral sclerosis occurring after forty. -Only one was possibly familial. -The onset is mostly with purely pyramidal features or of the topographically circumscribed peripheral type. Development appears to take longer than the average for amyotrophic lateral sclerosis. It would be worth while confirming these findings by analysis of other similar groups.", "contents": "[Amyotrophic lateral sclerosis occuring befor the age of 40 years. Remarks apropos of 25 cases]. Twenty-five cases of amyotrophic lateral sclerosis occurring before the age of 40, collected between 1963 and 1973 in the Neurological Hospital, Lyons, have prompted the authors to make the following observations. -The incidence of disease seems to have been on the increase during this period both in absolute terms and relatively speaking (relative to the total number of patients hospitalized and to the total number of patients hospitalized for amyotrophic lateral sclerosis after the age of forty). -The 25 cases involved mostly women--the opposite of what was found in cases of amyotrophic lateral sclerosis occurring after forty. -Only one was possibly familial. -The onset is mostly with purely pyramidal features or of the topographically circumscribed peripheral type. Development appears to take longer than the average for amyotrophic lateral sclerosis. It would be worth while confirming these findings by analysis of other similar groups.", "PMID": 981861}
{"id": "pubmed23n0032_12788", "title": "[Segmental muscular hypertrophy in Arnold-Chiari deformity associated with syringomyelia syndrome].", "content": "A case of Arnold-Chiari malformation plus syringomyelic syndrome with hypertrophy of upper left and lower right limbs is reported. The pathogenesis of this muscular hypertrophy is discussed. The authors retain the idea of an added malformation.", "contents": "[Segmental muscular hypertrophy in Arnold-Chiari deformity associated with syringomyelia syndrome]. A case of Arnold-Chiari malformation plus syringomyelic syndrome with hypertrophy of upper left and lower right limbs is reported. The pathogenesis of this muscular hypertrophy is discussed. The authors retain the idea of an added malformation.", "PMID": 981862}
{"id": "pubmed23n0032_12789", "title": "[Elimination by heat of false positive reactions during the detection of Australia antigen by passive hemagglutination tests].", "content": "The detection of the HB'S Antigen by a reversed passive haemagglutination test (H\u00e9panosticon) in sera previously heated at 56 degrees C for 30 mn, is simple and rapid but gives a relatively high number of false positive results. A simple technique is suggested by heating sera betwen 68 degrees C and 80 degrees C and then by centrifugation at 1.500 g for 15 mn, to eliminate these false positive results. The supernatants are tested in the Hepanosticon technique and only the true positive samples, cheked for specificity by other techniques namely radioimmuno assay (R.I.A.) remain positive. Further research is pursued to confirm these first data and to define the exact degree of sensibility of this technique.", "contents": "[Elimination by heat of false positive reactions during the detection of Australia antigen by passive hemagglutination tests]. The detection of the HB'S Antigen by a reversed passive haemagglutination test (H\u00e9panosticon) in sera previously heated at 56 degrees C for 30 mn, is simple and rapid but gives a relatively high number of false positive results. A simple technique is suggested by heating sera betwen 68 degrees C and 80 degrees C and then by centrifugation at 1.500 g for 15 mn, to eliminate these false positive results. The supernatants are tested in the Hepanosticon technique and only the true positive samples, cheked for specificity by other techniques namely radioimmuno assay (R.I.A.) remain positive. Further research is pursued to confirm these first data and to define the exact degree of sensibility of this technique.", "PMID": 981870}
{"id": "pubmed23n0032_12790", "title": "[Post-tranfusional malaria in France].", "content": "A global review of the porblem of malaria accidentally transmitted by blood transfusion is reported in France during these ten last years. Biological and epidemiological studies of malariae are recorded. Among the factors involved in the transmission of malariae by blood transfuction, the persistence of the parasites in the donors is very important. It is often longer than usual in the subjects who have lived in endemic malarious areas for many years or are always travelling in these countries, because they are partly protected by their acquired immunity and may be carriers of asymptomatic infection. The viability of parasites in stored blood, red cells and preparations with platelets, leucocytes or plasma containing a few red cells with parasites is discussed. The prevention depended on the elimination of any blood donor who has ever had malaria appears to be the simplest method but it is also the most failible. The screening of donors by direct microscopy is obviously impracticable because of low density and often submicroscopic level of their parasitoemia. Screening donors by IFA test is the best method. If IFA test is negative four months after coming back from endemic areas and two months after ending suppressive therapeutic, whole blood, red cells, leucocytes, platelets and fresh plasma can be used on condition that the stay of donors in endemic areas had been not too long. If the stay in these countries was very long, blood will be only used for preparing lyophilized plasma, fibrinogen immunoglobulins or albumin.", "contents": "[Post-tranfusional malaria in France]. A global review of the porblem of malaria accidentally transmitted by blood transfusion is reported in France during these ten last years. Biological and epidemiological studies of malariae are recorded. Among the factors involved in the transmission of malariae by blood transfuction, the persistence of the parasites in the donors is very important. It is often longer than usual in the subjects who have lived in endemic malarious areas for many years or are always travelling in these countries, because they are partly protected by their acquired immunity and may be carriers of asymptomatic infection. The viability of parasites in stored blood, red cells and preparations with platelets, leucocytes or plasma containing a few red cells with parasites is discussed. The prevention depended on the elimination of any blood donor who has ever had malaria appears to be the simplest method but it is also the most failible. The screening of donors by direct microscopy is obviously impracticable because of low density and often submicroscopic level of their parasitoemia. Screening donors by IFA test is the best method. If IFA test is negative four months after coming back from endemic areas and two months after ending suppressive therapeutic, whole blood, red cells, leucocytes, platelets and fresh plasma can be used on condition that the stay of donors in endemic areas had been not too long. If the stay in these countries was very long, blood will be only used for preparing lyophilized plasma, fibrinogen immunoglobulins or albumin.", "PMID": 981871}
{"id": "pubmed23n0032_12791", "title": "[Clinical symptoms of transfusional malaria].", "content": "Tranfusional malaria in countries located outside zones of endemia is difficult to evidence because it is rarely aparent. The clinic can be compared to that of malaria through inoculation by anophele, but it can be difficult to evidence because of its intricacy with the symptomatology of the affection which justified the transfusion. Usually, it takes the appearance of a febril gastric problem, but it may have other aspects: intestinal, hepato-biliar, neurologic or hematologic symptoms. As soon as the diagnosis is established, it is easy to confirm by a cytologic test on peripheric blood and/or on bone marrow and specially by a sero diagnosis using immuno fluorescence.", "contents": "[Clinical symptoms of transfusional malaria]. Tranfusional malaria in countries located outside zones of endemia is difficult to evidence because it is rarely aparent. The clinic can be compared to that of malaria through inoculation by anophele, but it can be difficult to evidence because of its intricacy with the symptomatology of the affection which justified the transfusion. Usually, it takes the appearance of a febril gastric problem, but it may have other aspects: intestinal, hepato-biliar, neurologic or hematologic symptoms. As soon as the diagnosis is established, it is easy to confirm by a cytologic test on peripheric blood and/or on bone marrow and specially by a sero diagnosis using immuno fluorescence.", "PMID": 981872}
{"id": "pubmed23n0032_12792", "title": "[Prevention of post-transfusional malaria by sero-detection of latent Plasmodium carriers among blood donors].", "content": "The risks of post-transfusion malaria are becoming a worry and the sero-investigation of latent carriers of plasmodium, among donors, is certainly the only efficient prophylactic measure. This is the result of an investigation carried out between October 1973 and February 1975 in 18 Blood Tranfusion Centres in France. Out of 2.997 sera studied in immuno-fluorescence of malaria, 3 to 5.2% of sero-positivity have been noticed, depending on the antigen used (P. falciparum, P. malariae and P. cynomolgi bastianellii). If the presence of fluorescent antibodies --  especially at weak titers --, does not mean compulsorily that the parasitemia persists, the serologic negativity leads to a diagnosis of exclusion. In this manner, the idea of a latent malaria is eliminated and one can determine precisely which bloods will be transfused without danger. But the required condition is that the sero-diagnosis of malaria be done on homologous antigens, which is, in spite of various technical difficulties, realizable in specialized laboratories. For material reasons, these tests cannot applied to all donors who have lived overseas. In return, it would be indubitably desired that these tests be done, among these donors, on subjects belonging to rare blood groups.", "contents": "[Prevention of post-transfusional malaria by sero-detection of latent Plasmodium carriers among blood donors]. The risks of post-transfusion malaria are becoming a worry and the sero-investigation of latent carriers of plasmodium, among donors, is certainly the only efficient prophylactic measure. This is the result of an investigation carried out between October 1973 and February 1975 in 18 Blood Tranfusion Centres in France. Out of 2.997 sera studied in immuno-fluorescence of malaria, 3 to 5.2% of sero-positivity have been noticed, depending on the antigen used (P. falciparum, P. malariae and P. cynomolgi bastianellii). If the presence of fluorescent antibodies --  especially at weak titers --, does not mean compulsorily that the parasitemia persists, the serologic negativity leads to a diagnosis of exclusion. In this manner, the idea of a latent malaria is eliminated and one can determine precisely which bloods will be transfused without danger. But the required condition is that the sero-diagnosis of malaria be done on homologous antigens, which is, in spite of various technical difficulties, realizable in specialized laboratories. For material reasons, these tests cannot applied to all donors who have lived overseas. In return, it would be indubitably desired that these tests be done, among these donors, on subjects belonging to rare blood groups.", "PMID": 981873}
{"id": "pubmed23n0032_12793", "title": "[Trial serologic detection of Plasmodium carriers among blood donors of the Centre de Transfusion Sanguine de Marseiile].", "content": "This paper is a survey of possible reservoir hosts of Plasmodium among donors of the blood transfusion Center of Marseille, by IFA test with P. falciparum and P. cynomolgi bastianellii antigens. It was found 15,7 per cent of positivity among 644 sera carefully selected during 6 months. Nord Africa and West Africa were the most dangerous countries, the former because of the great number of donors (332 of 744), the later because of the high rate (15,8 per cent) of positivity. The incidence of the say (frequency and long time) in infested countries and the interval from tate (15,8 per cent) of positivity. The incidence of the stay (frequency and long time) in infested countries and the interval from the last infestation possibility to the IFA test are very important factors. A prevention scheme is proposed: The donors staying during a very long time in infested countries will generally be used only for preparing plasma fractions by ethanol precipitations. For the others donors the blood will be used for preparing whole blood, red cells, fresh or frozen plasma, platelets and leucocytes preparation, in which a few parasites can be present, inly if a IFA test is negative 4 months after their coming back in our country.", "contents": "[Trial serologic detection of Plasmodium carriers among blood donors of the Centre de Transfusion Sanguine de Marseiile]. This paper is a survey of possible reservoir hosts of Plasmodium among donors of the blood transfusion Center of Marseille, by IFA test with P. falciparum and P. cynomolgi bastianellii antigens. It was found 15,7 per cent of positivity among 644 sera carefully selected during 6 months. Nord Africa and West Africa were the most dangerous countries, the former because of the great number of donors (332 of 744), the later because of the high rate (15,8 per cent) of positivity. The incidence of the say (frequency and long time) in infested countries and the interval from tate (15,8 per cent) of positivity. The incidence of the stay (frequency and long time) in infested countries and the interval from the last infestation possibility to the IFA test are very important factors. A prevention scheme is proposed: The donors staying during a very long time in infested countries will generally be used only for preparing plasma fractions by ethanol precipitations. For the others donors the blood will be used for preparing whole blood, red cells, fresh or frozen plasma, platelets and leucocytes preparation, in which a few parasites can be present, inly if a IFA test is negative 4 months after their coming back in our country.", "PMID": 981875}
{"id": "pubmed23n0032_12794", "title": "[Results of a survey on post-transfusional malaria in France between 1960 and 1974].", "content": "This inquire demonstrated that transfusion malaria was not so rare as we thought. So 56 cases have been numbered between 1960 and 1974, and probably they do not reflect exactly the real situation. In our country, plasmodium falciparum, specifically responsible of the most serious accidents, is the form most frequently incriminated and its frequency increases among the years: 76,1% of the infects between 1970 and 1974. At last we have confirmed that a prevent of those accidents must consider as a subject any one having resided in an impaluded country, and that implies a constant knowledge of the epidemiologic map of this parisitism. This prevent will be increased so that the transfusion malaria will not get a illness disease in France.", "contents": "[Results of a survey on post-transfusional malaria in France between 1960 and 1974]. This inquire demonstrated that transfusion malaria was not so rare as we thought. So 56 cases have been numbered between 1960 and 1974, and probably they do not reflect exactly the real situation. In our country, plasmodium falciparum, specifically responsible of the most serious accidents, is the form most frequently incriminated and its frequency increases among the years: 76,1% of the infects between 1970 and 1974. At last we have confirmed that a prevent of those accidents must consider as a subject any one having resided in an impaluded country, and that implies a constant knowledge of the epidemiologic map of this parisitism. This prevent will be increased so that the transfusion malaria will not get a illness disease in France.", "PMID": 981874}
{"id": "pubmed23n0032_12795", "title": "[Radiation-induced teratogen effects and therapeutic abortion (author's transl)].", "content": "Ionizing radiation during the whole developmental phase induces high radiation sensitivity in the human embryo and fetus. Particularly in the phase of organogenesis, increased malformations result in a lower quality of life for the fetus after birth. In a suspected or existing pregnancy, it is, therefore, necessary to limit the indication for examination or treatment of the mother with ionizing radiation or radioactive elements more than in other phases of life. The probability of unfavorably influencing development in the womb by ionizing radiation depends on the dose received by the fetus. For this reason, all measures should be taken to keep the radiation exposure to a minimum. Since up until now no uniform recommendations regarding indication for therapeutic abortion following irradiation have been drawn up in Western Germany, it is advisable to fall back on those recommendations drawn up by Scandinavian countries on the basis of thorough radiobiological knowledge and experience. In cases where the probability of unfavorable effects exceeds 10% and/or the natural malformation rate doubles, a therapeutic abortion should be considered. In a properly conducted examination, this level is generally not reached. A few tips are also presented in regard to the proper execution of the examination during pregnancy.", "contents": "[Radiation-induced teratogen effects and therapeutic abortion (author's transl)]. Ionizing radiation during the whole developmental phase induces high radiation sensitivity in the human embryo and fetus. Particularly in the phase of organogenesis, increased malformations result in a lower quality of life for the fetus after birth. In a suspected or existing pregnancy, it is, therefore, necessary to limit the indication for examination or treatment of the mother with ionizing radiation or radioactive elements more than in other phases of life. The probability of unfavorably influencing development in the womb by ionizing radiation depends on the dose received by the fetus. For this reason, all measures should be taken to keep the radiation exposure to a minimum. Since up until now no uniform recommendations regarding indication for therapeutic abortion following irradiation have been drawn up in Western Germany, it is advisable to fall back on those recommendations drawn up by Scandinavian countries on the basis of thorough radiobiological knowledge and experience. In cases where the probability of unfavorable effects exceeds 10% and/or the natural malformation rate doubles, a therapeutic abortion should be considered. In a properly conducted examination, this level is generally not reached. A few tips are also presented in regard to the proper execution of the examination during pregnancy.", "PMID": 981896}
{"id": "pubmed23n0032_12796", "title": "[Changes of cutaneous blood flow after aortofemoral angiography (author's transl)].", "content": "Temperature versus time curves were registered during aortofemoral angiography in 48 patients. The cutaneous temperature was initially reduced in 46 of the patients. This phenomenon was interpreted as a temporary decrease of blood flow and can not be explained by hypertonicity of contrast media, but indicates additional pharmacodynamic effects. Reactive hyperemia depends on pathologic alterations of large and small vessels.", "contents": "[Changes of cutaneous blood flow after aortofemoral angiography (author's transl)]. Temperature versus time curves were registered during aortofemoral angiography in 48 patients. The cutaneous temperature was initially reduced in 46 of the patients. This phenomenon was interpreted as a temporary decrease of blood flow and can not be explained by hypertonicity of contrast media, but indicates additional pharmacodynamic effects. Reactive hyperemia depends on pathologic alterations of large and small vessels.", "PMID": 981897}
{"id": "pubmed23n0032_12797", "title": "[Surgical treatment of varices (author's transl)].", "content": "Basically three possibilities are available for the treatment of the varicose symptom complex: 1. Varicosclerozation, 2. Surgery, 3. Compression. Dermatologists and internists understandably prefer varicosclerozation; surgeons, surgery. The best success, in my opinion, is achieved with all three possibilities of treatment are used in combination and adjusted individually to the vascular findings of each case. There is no effective varicosclerozation and no surgical treatment without subsequent compression. In no case, can treatment by medication be more than supportive for other effective measures. The goal of this article is to provide information regarding the present status of surgical treatment. Unavoidably, the indication for the other methods of treatment was also established.", "contents": "[Surgical treatment of varices (author's transl)]. Basically three possibilities are available for the treatment of the varicose symptom complex: 1. Varicosclerozation, 2. Surgery, 3. Compression. Dermatologists and internists understandably prefer varicosclerozation; surgeons, surgery. The best success, in my opinion, is achieved with all three possibilities of treatment are used in combination and adjusted individually to the vascular findings of each case. There is no effective varicosclerozation and no surgical treatment without subsequent compression. In no case, can treatment by medication be more than supportive for other effective measures. The goal of this article is to provide information regarding the present status of surgical treatment. Unavoidably, the indication for the other methods of treatment was also established.", "PMID": 981898}
{"id": "pubmed23n0032_12798", "title": "[Significance of inhalation and perfusion scintigraphy for the diagnosis of pulmonary embolism (author's transl)].", "content": "Inhalation and perfusion scintigrams, general radiological examination of the thorax and an EKG were done on 43 patients with clinically suspected pulmonary embolism. In 11 cases, the clinical diagnosis could be confirmed on the basis of the scintigraphic examination. In the remaining 32 patients, an embolich event could be excluded by pulmonary scintigram, radiological examination and later the clinical course. A synopsis of clinical observations, X-ray and scintigraphy, therefore, permitted a basically reliable diagnosis of pulmonary embolism; selective pulmonary angiography should be used only in exceptional cases.", "contents": "[Significance of inhalation and perfusion scintigraphy for the diagnosis of pulmonary embolism (author's transl)]. Inhalation and perfusion scintigrams, general radiological examination of the thorax and an EKG were done on 43 patients with clinically suspected pulmonary embolism. In 11 cases, the clinical diagnosis could be confirmed on the basis of the scintigraphic examination. In the remaining 32 patients, an embolich event could be excluded by pulmonary scintigram, radiological examination and later the clinical course. A synopsis of clinical observations, X-ray and scintigraphy, therefore, permitted a basically reliable diagnosis of pulmonary embolism; selective pulmonary angiography should be used only in exceptional cases.", "PMID": 981899}
{"id": "pubmed23n0032_12799", "title": "[Comparative study of xeroradiography and filmradiography (2700 statistically evaluated tests) (author's transl)].", "content": "Bases on more than 2700 statistically evaluated, comparative filmradiographic thoracic organs, abdomen and vascular and lymphatic system, the value of xeroradiography was analyzed by a standardized process. We came to the conclusion that, in certain cases, xeroradiography is superior (example: head-neck-soft tissues), equal (example: cranial area) or inferior (example: abdomen) to conventional X-ray film techniques. The xeroradiographic procedure has advantages for the radiologist if he does not try to use it as a substitute for the conventional X-ray film technique.", "contents": "[Comparative study of xeroradiography and filmradiography (2700 statistically evaluated tests) (author's transl)]. Bases on more than 2700 statistically evaluated, comparative filmradiographic thoracic organs, abdomen and vascular and lymphatic system, the value of xeroradiography was analyzed by a standardized process. We came to the conclusion that, in certain cases, xeroradiography is superior (example: head-neck-soft tissues), equal (example: cranial area) or inferior (example: abdomen) to conventional X-ray film techniques. The xeroradiographic procedure has advantages for the radiologist if he does not try to use it as a substitute for the conventional X-ray film technique.", "PMID": 981900}
{"id": "pubmed23n0032_12800", "title": "[X-ray protection zones during x-ray examens in intensive care (author's transl)].", "content": "For x-ray examens in intensiv care units is shown that in a distance of 1,5 m or 2,0 m the registered doses are so low that the x-ray protection zones could be limeted to this area.", "contents": "[X-ray protection zones during x-ray examens in intensive care (author's transl)]. For x-ray examens in intensiv care units is shown that in a distance of 1,5 m or 2,0 m the registered doses are so low that the x-ray protection zones could be limeted to this area.", "PMID": 981901}
{"id": "pubmed23n0032_12801", "title": "[Joint manifestations of Yersinia enterocolitica infections].", "content": "Different recent studies, notably Scandinavian studies, have described a \"rheumatism\" caused by Yersinia enterocolitica infection. The picture usually seen is that of a subacute, febrile oligoarthritis predominantly in the lower limbs, associated with diarrhoea and hyperleucocytosis, polynucleosis, and a marked increase in the sedimentation rate. Benign carditis may also be associated. The diagnosis rests on the discovery of the organism by coproculture and on serodiagnosis. Evolution is favourable within several weeks or several months. Treatment includes antibiotics and antinflammatory preparations.", "contents": "[Joint manifestations of Yersinia enterocolitica infections]. Different recent studies, notably Scandinavian studies, have described a \"rheumatism\" caused by Yersinia enterocolitica infection. The picture usually seen is that of a subacute, febrile oligoarthritis predominantly in the lower limbs, associated with diarrhoea and hyperleucocytosis, polynucleosis, and a marked increase in the sedimentation rate. Benign carditis may also be associated. The diagnosis rests on the discovery of the organism by coproculture and on serodiagnosis. Evolution is favourable within several weeks or several months. Treatment includes antibiotics and antinflammatory preparations.", "PMID": 981921}
{"id": "pubmed23n0032_12802", "title": "[Joint manifestations of carcinoid tumors of the digestive system].", "content": "The joint manifestations of carcinoid tumours become integrated into the carcinoid syndrome, the totality of the symptoms occurring at a distance from the tumour and linked to the metabolites secreted by the tumour. These arthropathies have been the subject of only a very limited number of publications. They consist of arthralgia, sometimes with swelling, predominantly in the extremities of the upper limbs, and linked to activity. The radiological picture includes a \"band\" of demineralization, and less frequently destructive joint lesions. The sedimentation rate is always increased. These arthropathies can be related to their origin only by knowledge of, and demonstration of, the other elements of the carcinoid syndrome and of the tumoro-metastic syndrome. Laboratory diagnosis rests on determination of the 5-hydroxyl derivatives of tryptophane.", "contents": "[Joint manifestations of carcinoid tumors of the digestive system]. The joint manifestations of carcinoid tumours become integrated into the carcinoid syndrome, the totality of the symptoms occurring at a distance from the tumour and linked to the metabolites secreted by the tumour. These arthropathies have been the subject of only a very limited number of publications. They consist of arthralgia, sometimes with swelling, predominantly in the extremities of the upper limbs, and linked to activity. The radiological picture includes a \"band\" of demineralization, and less frequently destructive joint lesions. The sedimentation rate is always increased. These arthropathies can be related to their origin only by knowledge of, and demonstration of, the other elements of the carcinoid syndrome and of the tumoro-metastic syndrome. Laboratory diagnosis rests on determination of the 5-hydroxyl derivatives of tryptophane.", "PMID": 981923}
{"id": "pubmed23n0032_12803", "title": "[Arthrotic sciatica caused by radicular compression of osteophytic origin in the lateral recess. Apropos of 18 cases].", "content": "The authors report 18 cases of arthrosic sciatica due to toot compression in the lateral recess by posterior corporeal and/or posterior apophyseal osteophytosis. The authors study the clinical and radiological characteristics that may indicate the diagnosis and discuss the different mechanisms by which vertebral arthrosis can lead to radicular compression. When surgery is necessary because of the persistent nature of the sciatica, a broader approach should be undertaken than that required for excision of the disc, in order to explore fully the roots, the multiplicity of possible compression sites being one of the essential characteristics of these cases of arthrosic radiculopathy. Study of the literature and of the series of sciatica patients operated upon by the authors shows that although discal hernia is far from being the most frequent cause of common sciatica, arthrosic compression is a cause that cannot be ignored, especially in aged subjects.", "contents": "[Arthrotic sciatica caused by radicular compression of osteophytic origin in the lateral recess. Apropos of 18 cases]. The authors report 18 cases of arthrosic sciatica due to toot compression in the lateral recess by posterior corporeal and/or posterior apophyseal osteophytosis. The authors study the clinical and radiological characteristics that may indicate the diagnosis and discuss the different mechanisms by which vertebral arthrosis can lead to radicular compression. When surgery is necessary because of the persistent nature of the sciatica, a broader approach should be undertaken than that required for excision of the disc, in order to explore fully the roots, the multiplicity of possible compression sites being one of the essential characteristics of these cases of arthrosic radiculopathy. Study of the literature and of the series of sciatica patients operated upon by the authors shows that although discal hernia is far from being the most frequent cause of common sciatica, arthrosic compression is a cause that cannot be ignored, especially in aged subjects.", "PMID": 981929}
{"id": "pubmed23n0032_12804", "title": "[Patellar malposition, its incidence in patellar chondropathy and in a control population].", "content": "Patellar disequilibrium consists of either external malpositioning of the patella (EMP) or external hyperpression (EHP), which are clearly visible on a 30degrees axial radiograph of the knee. Does this disequilibrium play a role in the development of patellar chondropathy? The authors have based their comparisons on a series of 40 subjects more than 60 years of age (average age: 68 years) who were free from pain and arthrosis of the knee. In this group they found 2 cases of EMP (5 percent) and 3 of EHP (7.5 percent). In their series of cases of patellar chondropathy (43 cases) there were, after correction for the cases with a double etiology, 8.5 cases of EMP (20 percent) and 7 of EHP (16 percent). The differences between these figures and those for the control series are statistically significant for the number of cases of EMP (P less than 0.05), but not for the number of cases of EHP, the etiological role of which is probable but for which proof would require a larger series of patients. From this work the following findings emerge: (1) The frequency of latent patellofemoral arthrosis in the aged control subjects with knee problems. 7 cases out of 47, or 12.5 percent. (2) The frequency of patellar chondropathy apparently associated with the two etiological factors: out of 43 patients, 22 suffered from patellar disequilibrium (10 EMP and 12 EHP) but in 13 cases there was another associated possible cause of deterioration: traumatism or stress on the knee associated with work or sport. This methodological difficulty is discussed and resolved empirically. The importance of patellar disequilibrium in the development of degenerative patellofemoral affections derives not from the etiological importance but from the fact that they represent the only factor for which therapy may be given: elective internal rehabilitation or surgery (section of the external wing of the patella or transposition of the anterior tuberosity of the tibia).", "contents": "[Patellar malposition, its incidence in patellar chondropathy and in a control population]. Patellar disequilibrium consists of either external malpositioning of the patella (EMP) or external hyperpression (EHP), which are clearly visible on a 30degrees axial radiograph of the knee. Does this disequilibrium play a role in the development of patellar chondropathy? The authors have based their comparisons on a series of 40 subjects more than 60 years of age (average age: 68 years) who were free from pain and arthrosis of the knee. In this group they found 2 cases of EMP (5 percent) and 3 of EHP (7.5 percent). In their series of cases of patellar chondropathy (43 cases) there were, after correction for the cases with a double etiology, 8.5 cases of EMP (20 percent) and 7 of EHP (16 percent). The differences between these figures and those for the control series are statistically significant for the number of cases of EMP (P less than 0.05), but not for the number of cases of EHP, the etiological role of which is probable but for which proof would require a larger series of patients. From this work the following findings emerge: (1) The frequency of latent patellofemoral arthrosis in the aged control subjects with knee problems. 7 cases out of 47, or 12.5 percent. (2) The frequency of patellar chondropathy apparently associated with the two etiological factors: out of 43 patients, 22 suffered from patellar disequilibrium (10 EMP and 12 EHP) but in 13 cases there was another associated possible cause of deterioration: traumatism or stress on the knee associated with work or sport. This methodological difficulty is discussed and resolved empirically. The importance of patellar disequilibrium in the development of degenerative patellofemoral affections derives not from the etiological importance but from the fact that they represent the only factor for which therapy may be given: elective internal rehabilitation or surgery (section of the external wing of the patella or transposition of the anterior tuberosity of the tibia).", "PMID": 981927}
{"id": "pubmed23n0032_12805", "title": "[HL-A and chronic juvenile polyarthritis].", "content": "The authors determined the HLA groups in 1000 blood donors, in 62 subjects suffering from ankylosing spondylarthritis, and in 32 subjects suffering from chronic juvenile polyarthritis. The results confirm those of other authors as regards the occurrence of W27 antigen in ankylosing spondylarthritis. The examination of the subjects suffering from chronic juvenile polyarthritis made it possible to subdivide this population into 4 groups: (1) Chronic juvenile polyarthritis evolving towards ankylosing spondylarthritis; (2) Chronic juvenile polyarthritis with sacroilitis; (3) Chronic juvenile polyarthritis without sacroiliac involvement; and (4) Rhematoid juvenile polyarthritis. They showed that the presence of W27 antigen was very high in groups (1) and (2) and very low in groups (3) and (4). Determination of the presence of W27 antigen makes it possible to place the patient in one of these groups and the future will show whether this classification is of any therapeutic consequence.", "contents": "[HL-A and chronic juvenile polyarthritis]. The authors determined the HLA groups in 1000 blood donors, in 62 subjects suffering from ankylosing spondylarthritis, and in 32 subjects suffering from chronic juvenile polyarthritis. The results confirm those of other authors as regards the occurrence of W27 antigen in ankylosing spondylarthritis. The examination of the subjects suffering from chronic juvenile polyarthritis made it possible to subdivide this population into 4 groups: (1) Chronic juvenile polyarthritis evolving towards ankylosing spondylarthritis; (2) Chronic juvenile polyarthritis with sacroilitis; (3) Chronic juvenile polyarthritis without sacroiliac involvement; and (4) Rhematoid juvenile polyarthritis. They showed that the presence of W27 antigen was very high in groups (1) and (2) and very low in groups (3) and (4). Determination of the presence of W27 antigen makes it possible to place the patient in one of these groups and the future will show whether this classification is of any therapeutic consequence.", "PMID": 981931}
{"id": "pubmed23n0032_12806", "title": "[Quantitative bone biopsy and primary hyperparathyroidism, its role in diagnosis. Apropos of 45 cases].", "content": "Iliac bone biopsy, including a quantitative study, was carried out in 38 patients with primary hyperparathyroidism (34 surgical patients, 4 with typical syndromes based on laboratory analysis). A second biopsy was carried out in 7 cases. By studying both the surface resorption of the strands of the spongy tissue and periosteocyte osteolysis (an indirect measure by assessment of the surface aera of the periosteocyte lacunae), it is possible to arrive at a histological diagnosis of hyperparathyroidism in 98 percent of cases: surface resorption is increased in 82 percent of cases; the periosteocyte lacunae are enlarged in 91 percent of cases; the two criteria together give a positive result in 44 cases out of 45 (98 percent). This morphometric study is particularly valuable in those cases in which the qualitative histological appearance is not very informative.", "contents": "[Quantitative bone biopsy and primary hyperparathyroidism, its role in diagnosis. Apropos of 45 cases]. Iliac bone biopsy, including a quantitative study, was carried out in 38 patients with primary hyperparathyroidism (34 surgical patients, 4 with typical syndromes based on laboratory analysis). A second biopsy was carried out in 7 cases. By studying both the surface resorption of the strands of the spongy tissue and periosteocyte osteolysis (an indirect measure by assessment of the surface aera of the periosteocyte lacunae), it is possible to arrive at a histological diagnosis of hyperparathyroidism in 98 percent of cases: surface resorption is increased in 82 percent of cases; the periosteocyte lacunae are enlarged in 91 percent of cases; the two criteria together give a positive result in 44 cases out of 45 (98 percent). This morphometric study is particularly valuable in those cases in which the qualitative histological appearance is not very informative.", "PMID": 981932}
{"id": "pubmed23n0032_12807", "title": "[Study of circulating 25 hydroxyvitamin D].", "content": "The levels of circulating 25 OH-D were determined by a direct radio-competition methods both in normal subjects and in subjects with various pathological conditions. In normal subjects, the average level of 25 OH-D was higher in summer (42.3 ng/ml) than in winter (29.1 ng/ml), P less than 0.005. Monthly variations in the 25 HO-D levels were found in relation to insolation The level of 25 OH-D was practically normal in osteoporosis (28.9 ng/ml), clearly lower in the mixed forms called \"osteoporomalacia\" (13.5 ng/ml, P less than 0.005) and very low in osteomalacia (5.8 ng/ml, P less than 0.001). In cases of cortisone osteopathy the average level was 22.8 ng/ml (NS). The level of 25 OH-D was also found to be lower in hepatic cirrhosis (11.7 ng/ml, P less than 0.01), in subjects treated with anticonvulsants (P less than 0.01), and in the course of hyperparathyroidism (P less than 0.002). There was no corelation between the level of 25 OH-D and calcaemia, phosphoraemia, circulating immunoreactive parathyroid hormone, or the relative osteoid volume. In contrast, there seemed to be a good correlation with the level of alkaline phosphatasaemia. The level of 25 OH-D was also determined in 4 subjects with vitamin-resistant osteomalacia: in 3 cases hepatic hydroxylation seemed normal, indicating the possibility of a subsequent disorder of vitamin D metabolism; in one case the absence of hepatic hydroxylation was noted.", "contents": "[Study of circulating 25 hydroxyvitamin D]. The levels of circulating 25 OH-D were determined by a direct radio-competition methods both in normal subjects and in subjects with various pathological conditions. In normal subjects, the average level of 25 OH-D was higher in summer (42.3 ng/ml) than in winter (29.1 ng/ml), P less than 0.005. Monthly variations in the 25 HO-D levels were found in relation to insolation The level of 25 OH-D was practically normal in osteoporosis (28.9 ng/ml), clearly lower in the mixed forms called \"osteoporomalacia\" (13.5 ng/ml, P less than 0.005) and very low in osteomalacia (5.8 ng/ml, P less than 0.001). In cases of cortisone osteopathy the average level was 22.8 ng/ml (NS). The level of 25 OH-D was also found to be lower in hepatic cirrhosis (11.7 ng/ml, P less than 0.01), in subjects treated with anticonvulsants (P less than 0.01), and in the course of hyperparathyroidism (P less than 0.002). There was no corelation between the level of 25 OH-D and calcaemia, phosphoraemia, circulating immunoreactive parathyroid hormone, or the relative osteoid volume. In contrast, there seemed to be a good correlation with the level of alkaline phosphatasaemia. The level of 25 OH-D was also determined in 4 subjects with vitamin-resistant osteomalacia: in 3 cases hepatic hydroxylation seemed normal, indicating the possibility of a subsequent disorder of vitamin D metabolism; in one case the absence of hepatic hydroxylation was noted.", "PMID": 981930}
{"id": "pubmed23n0032_12808", "title": "[Intermittent treatment of Paget's disease of bone with calcitonin: histological study].", "content": "The authors report the results of a semi-quantitative histological study of bone carried out in 6 patients with Paget's disease treated with salmon calcitonin (about thirty injections of an average of 50 MRC units) over 8 to 14 weeks. The treatment led to a decrease in the resorption surfaces, in the number and the nucleation of the osteoclasts, and in the level of hydroxyprolinuria. Cessation of treatment led to an increase in these parameters, but starting from the fifth month after the cessation renewed improvement was noted, concerning in particular the hydroxyprolinuria and the nucleation of the osteoclasts and this lasted until the tenth month. The possibility of a prolonged action of calcitonin indicates that discontinuous therapy of Paget's disease should be considered (4 months per year, for example).", "contents": "[Intermittent treatment of Paget's disease of bone with calcitonin: histological study]. The authors report the results of a semi-quantitative histological study of bone carried out in 6 patients with Paget's disease treated with salmon calcitonin (about thirty injections of an average of 50 MRC units) over 8 to 14 weeks. The treatment led to a decrease in the resorption surfaces, in the number and the nucleation of the osteoclasts, and in the level of hydroxyprolinuria. Cessation of treatment led to an increase in these parameters, but starting from the fifth month after the cessation renewed improvement was noted, concerning in particular the hydroxyprolinuria and the nucleation of the osteoclasts and this lasted until the tenth month. The possibility of a prolonged action of calcitonin indicates that discontinuous therapy of Paget's disease should be considered (4 months per year, for example).", "PMID": 981933}
{"id": "pubmed23n0032_12809", "title": "[Further observations on the presence of iron in the articuler synovial membrane].", "content": "In 1971, a study of 66 synovial biopsy samples indicated that the presence of ferric deposits in the subintimal synovial tissue was of real semiological value in the diagnosis of rheumatoid synovitis. In 1975, a study of 228 synovial biopsy samples led the authors to reconsider this finding: the presence of ferric deposits in the subintimal synovial tissue was far from being a constant feature in rheumatoid synovitis and it was seen with similar frequency in other forms of chronic inflammatory rheumatism and also in several other joint conditions. The origin of the synovial iron and the influence it may have on the development of the rheumatoid processes remain obscure.", "contents": "[Further observations on the presence of iron in the articuler synovial membrane]. In 1971, a study of 66 synovial biopsy samples indicated that the presence of ferric deposits in the subintimal synovial tissue was of real semiological value in the diagnosis of rheumatoid synovitis. In 1975, a study of 228 synovial biopsy samples led the authors to reconsider this finding: the presence of ferric deposits in the subintimal synovial tissue was far from being a constant feature in rheumatoid synovitis and it was seen with similar frequency in other forms of chronic inflammatory rheumatism and also in several other joint conditions. The origin of the synovial iron and the influence it may have on the development of the rheumatoid processes remain obscure.", "PMID": 981928}
{"id": "pubmed23n0032_12810", "title": "Arterial-hepatic vein glucose differences in normal and diabetic man after a glucose infusion at rest and after exercise.", "content": "The role of the liver in the elimination of infused glucose during recovery after exercise was studied in 5 normal and 4 juvenile diabetic males. The results were compared with those of a resting experiment. The subjects exercised on the bicycle ergometer at a work rate requiring about 70% of their max. Vo2. 0.5 g glucose per kg body weight was infused as a single injection 15 min after the cessation of exercise. Arterial-hepatic vein (a-hv) glucose and lactate differences were measured repetitively both during exercise and throughout the 79 min recovery period. During exercise the a-hv glucose differences were negative and increased more than sixfold in the diabetic subjects and more than tenfold in the non-diabetic subjects. After the glucose infusion the a-hv glucose differences were still negative in the diabetic subjects, indicating a continued net release of glucose from the liver in spite of elevated arterial glucose concentrations. In the non-diabetic subjects there was a small uptake of glucose, accounting for about 0.5% of the total glucose load given. The a-hv lactate differences were larger in the diabetic subjects, indicating a greater dependence upon gluconeogenesis as compared with the non-diabetic subjects. It is concluded that the liver is of no importance in the diabetic subjects and of only slight importance in the non-diabetic subjects for the disposal of infused glucose during the initial hour of recovery after exercise.", "contents": "Arterial-hepatic vein glucose differences in normal and diabetic man after a glucose infusion at rest and after exercise. The role of the liver in the elimination of infused glucose during recovery after exercise was studied in 5 normal and 4 juvenile diabetic males. The results were compared with those of a resting experiment. The subjects exercised on the bicycle ergometer at a work rate requiring about 70% of their max. Vo2. 0.5 g glucose per kg body weight was infused as a single injection 15 min after the cessation of exercise. Arterial-hepatic vein (a-hv) glucose and lactate differences were measured repetitively both during exercise and throughout the 79 min recovery period. During exercise the a-hv glucose differences were negative and increased more than sixfold in the diabetic subjects and more than tenfold in the non-diabetic subjects. After the glucose infusion the a-hv glucose differences were still negative in the diabetic subjects, indicating a continued net release of glucose from the liver in spite of elevated arterial glucose concentrations. In the non-diabetic subjects there was a small uptake of glucose, accounting for about 0.5% of the total glucose load given. The a-hv lactate differences were larger in the diabetic subjects, indicating a greater dependence upon gluconeogenesis as compared with the non-diabetic subjects. It is concluded that the liver is of no importance in the diabetic subjects and of only slight importance in the non-diabetic subjects for the disposal of infused glucose during the initial hour of recovery after exercise.", "PMID": 981955}
{"id": "pubmed23n0032_12811", "title": "Factor VIII (AHG) levels in 1016 regular blood donors. The effects of age, sex, and ABO blood groups.", "content": "Factor VIII (AHG) activity was assayed in the plasma specimens from 1016 regular and controlled blood donors. Age and ABO blood groups had highly significant effects on factor VIII concentrations, whereas the effect of sex was not significant. The median in the donors of blood group O rose from 87% at the age of 20 to 119% at the age of 60 years, and the median in blood groups A and B+AB of corresponding ages rose from 108 to 147%. An interaction effect of age and blood groups was present only at the 5% level of significance. The present data and the previous findings of others suggest the existence of an association between high factor VIII levels and thrombotic states.", "contents": "Factor VIII (AHG) levels in 1016 regular blood donors. The effects of age, sex, and ABO blood groups. Factor VIII (AHG) activity was assayed in the plasma specimens from 1016 regular and controlled blood donors. Age and ABO blood groups had highly significant effects on factor VIII concentrations, whereas the effect of sex was not significant. The median in the donors of blood group O rose from 87% at the age of 20 to 119% at the age of 60 years, and the median in blood groups A and B+AB of corresponding ages rose from 108 to 147%. An interaction effect of age and blood groups was present only at the 5% level of significance. The present data and the previous findings of others suggest the existence of an association between high factor VIII levels and thrombotic states.", "PMID": 981956}
{"id": "pubmed23n0032_12812", "title": "A simple method for measurement of angiotensin II in plasma.", "content": "A simple radioimmunological (RIA) method for the determination of angiotensin II in 0.5-1.0 ml samples of plasma is described and carefully evaluated. Before RIA was performed, the interfering plasma proteins were eliminated by ion exchange chromatography, and recovery from every column was checked with a small amount of [125I]angiotensin II. The sensitivity of the method was 4.0 ng/l; the coefficient of intra-assay variation was 10.0% and that of inter-assay variation 12.1%. Accuracy was studied both by adding various amounts of angiotensin II to plasma samples and by diluting plasma containing angiotensin II with the RIA buffer. Both studies gave very good correlations between found and expected values (r=0.998 and r=0.987). In a normal material (n=36), the mean angiotensin II concentration at 8 a.m. after 2 h ambulation 42.4 +/- 12.8 (S.D.) ng/l. Because the present method is accurate, precise, and practical, and allows measurement of angiotensin II in small samples, it seems useful for routine as well as for research work.", "contents": "A simple method for measurement of angiotensin II in plasma. A simple radioimmunological (RIA) method for the determination of angiotensin II in 0.5-1.0 ml samples of plasma is described and carefully evaluated. Before RIA was performed, the interfering plasma proteins were eliminated by ion exchange chromatography, and recovery from every column was checked with a small amount of [125I]angiotensin II. The sensitivity of the method was 4.0 ng/l; the coefficient of intra-assay variation was 10.0% and that of inter-assay variation 12.1%. Accuracy was studied both by adding various amounts of angiotensin II to plasma samples and by diluting plasma containing angiotensin II with the RIA buffer. Both studies gave very good correlations between found and expected values (r=0.998 and r=0.987). In a normal material (n=36), the mean angiotensin II concentration at 8 a.m. after 2 h ambulation 42.4 +/- 12.8 (S.D.) ng/l. Because the present method is accurate, precise, and practical, and allows measurement of angiotensin II in small samples, it seems useful for routine as well as for research work.", "PMID": 981957}
{"id": "pubmed23n0032_12813", "title": "Conductometric determination of CO2 in air samples.", "content": "A new procedure enables the CO2 concentrations of air samples to be determined and automatically registered. The CO2 contents of the air samples are absorbed in an Sr(OH)2 solution. The resulting decrease in electrical conductivity of the solution is automatically registered by a d.c. strip-chart recorder in such a way that the decline of the curve is proportional to the amount of CO2 absorbed. In each analysis, quantities of CO2 up to about 8 mumol can be determined with an error of less than 0.02 mumol, and, accordingly, CO2 quantities of 2 to 8 mumol contained in samples of at least 150 ml of fresh atmospheric air or about 1 ml of carboxygen or expired air can be analysed with an accuracy of better than +/- 1.0% of their CO2 content.", "contents": "Conductometric determination of CO2 in air samples. A new procedure enables the CO2 concentrations of air samples to be determined and automatically registered. The CO2 contents of the air samples are absorbed in an Sr(OH)2 solution. The resulting decrease in electrical conductivity of the solution is automatically registered by a d.c. strip-chart recorder in such a way that the decline of the curve is proportional to the amount of CO2 absorbed. In each analysis, quantities of CO2 up to about 8 mumol can be determined with an error of less than 0.02 mumol, and, accordingly, CO2 quantities of 2 to 8 mumol contained in samples of at least 150 ml of fresh atmospheric air or about 1 ml of carboxygen or expired air can be analysed with an accuracy of better than +/- 1.0% of their CO2 content.", "PMID": 981958}
{"id": "pubmed23n0032_12814", "title": "Metiamide inhibits canine gastric acid secretion evoked by sham feeding.", "content": "A continuous intravenous infusion of metiamide markedly inhibited the gastric acid secretory response to sham feeding in two dogs provided with Pavlov pouches and gastric fistulae. The results show that an antagonist of histamine H2-receptors is capable of inhibiting a vagally activated secretion evoked by sham feeding, which may be considered a physiological vagal stimulus.", "contents": "Metiamide inhibits canine gastric acid secretion evoked by sham feeding. A continuous intravenous infusion of metiamide markedly inhibited the gastric acid secretory response to sham feeding in two dogs provided with Pavlov pouches and gastric fistulae. The results show that an antagonist of histamine H2-receptors is capable of inhibiting a vagally activated secretion evoked by sham feeding, which may be considered a physiological vagal stimulus.", "PMID": 981959}
{"id": "pubmed23n0032_12815", "title": "Nephrotic syndrome in ulcerative colitis.", "content": "Complicating disorders in various organs outside the intestinal tract are common in ulcerative colitis. This report deals with the occurrence of nephrotic syndrome in two patients with long-standing ulcerative colitis. In the patient studied in most detail, 2 episodes have taken place, the first developing into uremia. After colectomy had been performed, rapid improvement of renal function took place. The morphological changes in kidney biopsies were compatible with the presence of focal glomerular sclerosis. Activity in the complement system and a favorable response to steroid treatment indicate that humoral immune mechanisms are of pathogenetic importance with regard to the renal disease in these two patients. To our knowledge nephrotic syndrome has not previously been described as a complication to ulcerative colitis.", "contents": "Nephrotic syndrome in ulcerative colitis. Complicating disorders in various organs outside the intestinal tract are common in ulcerative colitis. This report deals with the occurrence of nephrotic syndrome in two patients with long-standing ulcerative colitis. In the patient studied in most detail, 2 episodes have taken place, the first developing into uremia. After colectomy had been performed, rapid improvement of renal function took place. The morphological changes in kidney biopsies were compatible with the presence of focal glomerular sclerosis. Activity in the complement system and a favorable response to steroid treatment indicate that humoral immune mechanisms are of pathogenetic importance with regard to the renal disease in these two patients. To our knowledge nephrotic syndrome has not previously been described as a complication to ulcerative colitis.", "PMID": 981960}
{"id": "pubmed23n0032_12816", "title": "Intraluminal pressure in the sigmoid colon. I. Method and results in normal subjects.", "content": "A modified method for measurement of pressure activity in the sigmoid colon is presented, using open-ended tubes and measurement of pressure activity for 30 minutes after intravenous administration of 0.5 mg of neostigmine. The motility index in 12 normal persons was found to have median 597 with a range of 125-1169. Variation among persons exceeded the day-to-day variation significantly. Error in reading the curves was negligible.", "contents": "Intraluminal pressure in the sigmoid colon. I. Method and results in normal subjects. A modified method for measurement of pressure activity in the sigmoid colon is presented, using open-ended tubes and measurement of pressure activity for 30 minutes after intravenous administration of 0.5 mg of neostigmine. The motility index in 12 normal persons was found to have median 597 with a range of 125-1169. Variation among persons exceeded the day-to-day variation significantly. Error in reading the curves was negligible.", "PMID": 981961}
{"id": "pubmed23n0032_12817", "title": "Intraluminal pressure in the sigmoid colon. II. Patients with sigmoid diverticula and related conditions.", "content": "Patients with lower abdominal symptoms, indicating colonic disorder, were classified into predefined clinical syndromes. Two important syndromes were colicky sigmoid syndrome and chronic diverticular disease. The first one, characterized by presence of colicky lower abdominal pains but absence of colonic diverticula, probably covers what is generally referred to as 'irritable bowel syndrome'. The second one was characterized by presence of lower abdominal colics and of colonic diverticula. The results showed a significant correlation between the presence of lower abdominal colics and a high pressure activity in the sigmoid colon after intravenous neostigmine. There was, however, no correlation between the presence of diverticula and a high pressure activity. The generally accepted theory of a high pressure activity as the dominant factor in the pathogenesis of colonic diverticula, therefore, was questioned, as was the equally accepted theory of a gradual development of chronic diverticular disease from the adiverticular colicky sigmoid syndrome.", "contents": "Intraluminal pressure in the sigmoid colon. II. Patients with sigmoid diverticula and related conditions. Patients with lower abdominal symptoms, indicating colonic disorder, were classified into predefined clinical syndromes. Two important syndromes were colicky sigmoid syndrome and chronic diverticular disease. The first one, characterized by presence of colicky lower abdominal pains but absence of colonic diverticula, probably covers what is generally referred to as 'irritable bowel syndrome'. The second one was characterized by presence of lower abdominal colics and of colonic diverticula. The results showed a significant correlation between the presence of lower abdominal colics and a high pressure activity in the sigmoid colon after intravenous neostigmine. There was, however, no correlation between the presence of diverticula and a high pressure activity. The generally accepted theory of a high pressure activity as the dominant factor in the pathogenesis of colonic diverticula, therefore, was questioned, as was the equally accepted theory of a gradual development of chronic diverticular disease from the adiverticular colicky sigmoid syndrome.", "PMID": 981962}
{"id": "pubmed23n0032_12818", "title": "Incorporation rate in vitro of choline and methyl-methionine into human hepatic lecithins.", "content": "In order to evaluate the quantitative role of cytidinediphosphate-choline and phosphatidyl-ethanolamine-methylation pathways in the synthesis of phosphatidylcholine in human liver tissue, the incorporation rate of [14C]choline and [14C]methyl-methionine into liver lecithins was determined. Liver biopsy was taken in 27 patients operated upon for uncomplicated gallstone disease. Linear incorporation into lecithin was achieved for N-methyl-methionine during 4 hours' incubation and for choline from 7.5 min to 60 min of incubation but with a significant positive Y-intercept. This Y-intercept was directly proportional to the concentration of choline in the medium and might be explained by a rapid base exchange between free choline in the medium and bases in phosphoglycerides. Fertile women showed significantly higher incorporation rate of methyl-methionine than postmenopausal women. Bile acids in the incubation medium did not influence the incorporation rate of thesebation medium did not influence the incorporation rate of these precursors into hepatic lecithin: The results indicate that the contribution of the methylation pathway in the synthesis of human liver lecithin accounts for about 10-15 per cent of that of the CDP-choline pathway.", "contents": "Incorporation rate in vitro of choline and methyl-methionine into human hepatic lecithins. In order to evaluate the quantitative role of cytidinediphosphate-choline and phosphatidyl-ethanolamine-methylation pathways in the synthesis of phosphatidylcholine in human liver tissue, the incorporation rate of [14C]choline and [14C]methyl-methionine into liver lecithins was determined. Liver biopsy was taken in 27 patients operated upon for uncomplicated gallstone disease. Linear incorporation into lecithin was achieved for N-methyl-methionine during 4 hours' incubation and for choline from 7.5 min to 60 min of incubation but with a significant positive Y-intercept. This Y-intercept was directly proportional to the concentration of choline in the medium and might be explained by a rapid base exchange between free choline in the medium and bases in phosphoglycerides. Fertile women showed significantly higher incorporation rate of methyl-methionine than postmenopausal women. Bile acids in the incubation medium did not influence the incorporation rate of thesebation medium did not influence the incorporation rate of these precursors into hepatic lecithin: The results indicate that the contribution of the methylation pathway in the synthesis of human liver lecithin accounts for about 10-15 per cent of that of the CDP-choline pathway.", "PMID": 981963}
{"id": "pubmed23n0032_12819", "title": "Washing and direct vision brush cytology in the diagnosis of gastric carcinoma.", "content": "The diagnostic significance of direct vision brush cytology and simple washing cytology was studied in 3 series with gastric carcinoma verified at operation. Direct vision gastroscopic biopsy was performed in all patients. Both brush and washing cytology markedly increased the diagnostic reliability of gastroscopic biopsy. Wrong negative results (Papanicolau I-II) were found in 13% with brush cytology and in 34% with washing cytology. Direct comparison revealed that brush cytology was definitely superior to washing cytology in the diagnosis of gastric carcinoma. The results suggest a routine use of brush cytology in connection with gastroscopic biopsy.", "contents": "Washing and direct vision brush cytology in the diagnosis of gastric carcinoma. The diagnostic significance of direct vision brush cytology and simple washing cytology was studied in 3 series with gastric carcinoma verified at operation. Direct vision gastroscopic biopsy was performed in all patients. Both brush and washing cytology markedly increased the diagnostic reliability of gastroscopic biopsy. Wrong negative results (Papanicolau I-II) were found in 13% with brush cytology and in 34% with washing cytology. Direct comparison revealed that brush cytology was definitely superior to washing cytology in the diagnosis of gastric carcinoma. The results suggest a routine use of brush cytology in connection with gastroscopic biopsy.", "PMID": 981964}
{"id": "pubmed23n0032_12820", "title": "Dynamics of atrophic gastritis in male and female subjects after partial gastric resection -- an evaluation by stochastic analysis.", "content": "Forty-two subjects (33 male and 9 female) were examined 17 to 18 years after partial gastrectomy due to gastric or duodenal ulcer, with regard to morphological state of gastric body mucosa, specimens obtained by direct gastroscopy technique being evaluated by our scoring system. Our stochastic analysis was applied to the findings to obtain the \"age-adjusted score' (AAS), representing the degree of progression of chronic gastritis with reference to a random population, independent of the subject's age. Higher susceptibility of males than females to progressing chronic gastritis, and higher than that of both males and females of the random population, was noted. The atrophic changes in males after gastrectomy showed no dependence on age.", "contents": "Dynamics of atrophic gastritis in male and female subjects after partial gastric resection -- an evaluation by stochastic analysis. Forty-two subjects (33 male and 9 female) were examined 17 to 18 years after partial gastrectomy due to gastric or duodenal ulcer, with regard to morphological state of gastric body mucosa, specimens obtained by direct gastroscopy technique being evaluated by our scoring system. Our stochastic analysis was applied to the findings to obtain the \"age-adjusted score' (AAS), representing the degree of progression of chronic gastritis with reference to a random population, independent of the subject's age. Higher susceptibility of males than females to progressing chronic gastritis, and higher than that of both males and females of the random population, was noted. The atrophic changes in males after gastrectomy showed no dependence on age.", "PMID": 981965}
{"id": "pubmed23n0032_12821", "title": "Coenzyme-A-dependent esterification of cholesterol in rat intestinal mucosa.", "content": "Rat intestinal mucosa contains an enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA: cholesterol acyltransferase (ACAT) (E.C.2.3.1.26). Maximal activity in vitro is obtained when long chain acylcarnitines, CoA and carnitine palmityltransferase are used as an acyl-CoA generating system. The enzyme is localized in the microsomal fraction, has a pH optimum between 6.4-7.2, and oleate is the preferred acyl group. The activity of the enzyme is highest in the proximal jejunum, and has a capacity that can account for all cholesteryl esters found in intestinal rat lymph.", "contents": "Coenzyme-A-dependent esterification of cholesterol in rat intestinal mucosa. Rat intestinal mucosa contains an enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA: cholesterol acyltransferase (ACAT) (E.C.2.3.1.26). Maximal activity in vitro is obtained when long chain acylcarnitines, CoA and carnitine palmityltransferase are used as an acyl-CoA generating system. The enzyme is localized in the microsomal fraction, has a pH optimum between 6.4-7.2, and oleate is the preferred acyl group. The activity of the enzyme is highest in the proximal jejunum, and has a capacity that can account for all cholesteryl esters found in intestinal rat lymph.", "PMID": 981966}
{"id": "pubmed23n0032_12822", "title": "Early unconjugated hyperbilirubinaemia after paracetamol overdosage.", "content": "A rapid increase of predominantly unconjugated bilirubin in the blood -- which was not due to haemolysis -- was observed in 38 of 60 patients who had taken an overdose of paracetamol. Studies in the rat given an overdose of the drug showed that within 24 hours there was a striking reduction in the activity of bilirubin glucuronyl-transferase and the content of cytochrome P.450 in the liver. It is suggested that such early damage of the hepatic endoplasmic reticulum contributes to the hyperbilirubinaemia of paracetamol poisoning.", "contents": "Early unconjugated hyperbilirubinaemia after paracetamol overdosage. A rapid increase of predominantly unconjugated bilirubin in the blood -- which was not due to haemolysis -- was observed in 38 of 60 patients who had taken an overdose of paracetamol. Studies in the rat given an overdose of the drug showed that within 24 hours there was a striking reduction in the activity of bilirubin glucuronyl-transferase and the content of cytochrome P.450 in the liver. It is suggested that such early damage of the hepatic endoplasmic reticulum contributes to the hyperbilirubinaemia of paracetamol poisoning.", "PMID": 981967}
{"id": "pubmed23n0032_12823", "title": "Lower esophageal sphincter in reflux esophagitis.", "content": "Lower esophageal sphincter pressure (LESP) was studied by intraluminal perfusion-manometry in the resting state and after graded intravenous doses of pentagastrin in 12 healthy subjects and 27 patients with reflux esophagitis. These patients were classified as having ulcerative (11) or non-ulcerative esophagitis (16) by histological examination and endoscopic appearance of the inflamed mucosa. The length of the LES was similar in patients and controls. A correlation was found between the magnitude of mean basal end-expiratory (mbe) LESP reduction and the grade of esophageal mucosa inflammation. The most reduced mbe LESP value was shown in patients with ulcerative esophagitis. It was significantly different from that of patients with non-ulcerative esophagitis (t = 3.37, p less than 0.01). Both values were significantly reduced from that of the controls (t larger than or equal to 0.0125). The responsiveness of the LES to intravenous pentagastrin was quantitatively reduced throughout the whole range of the dose-response curve in patients with ulcerative esophagitis, whereas patients with non-ulcerative esophagitis only differed in the maximum mbe pressure value from the controls. Four patients showed a paradoxical pressure decrease to intravenous pentagastrin, resulting in a LESP reduction to 8% of the mbe LESP. Intravenous secretin or CCK led to a similar reaction of the LES in these patients.", "contents": "Lower esophageal sphincter in reflux esophagitis. Lower esophageal sphincter pressure (LESP) was studied by intraluminal perfusion-manometry in the resting state and after graded intravenous doses of pentagastrin in 12 healthy subjects and 27 patients with reflux esophagitis. These patients were classified as having ulcerative (11) or non-ulcerative esophagitis (16) by histological examination and endoscopic appearance of the inflamed mucosa. The length of the LES was similar in patients and controls. A correlation was found between the magnitude of mean basal end-expiratory (mbe) LESP reduction and the grade of esophageal mucosa inflammation. The most reduced mbe LESP value was shown in patients with ulcerative esophagitis. It was significantly different from that of patients with non-ulcerative esophagitis (t = 3.37, p less than 0.01). Both values were significantly reduced from that of the controls (t larger than or equal to 0.0125). The responsiveness of the LES to intravenous pentagastrin was quantitatively reduced throughout the whole range of the dose-response curve in patients with ulcerative esophagitis, whereas patients with non-ulcerative esophagitis only differed in the maximum mbe pressure value from the controls. Four patients showed a paradoxical pressure decrease to intravenous pentagastrin, resulting in a LESP reduction to 8% of the mbe LESP. Intravenous secretin or CCK led to a similar reaction of the LES in these patients.", "PMID": 981968}
{"id": "pubmed23n0032_12824", "title": "Reversible activation of proactivator (factor B) of the alternative pathway without cleavage of the molecule.", "content": "The serum of a patient (M.C.) with chronic glomerulonephritis and renal deposits of IgA, C3, and properdin converted C3 on overnight exposure of 0 degrees C. The cold reaction was dependent on immunoglobulin, initiating factor, Factors B and D, and magnesium but not on properdin. Factor B, the C3-cleaving enzyme in this reaction, was used in zymogen form. After participation in this rection, Factor B zymogen in M.C. serum could be fully activated by cobra venom factor (CVF) at 37 degrees C. That activation without fragmentation was not due to an abnormal form of Factor B was shown by its typical cleavage on incubation of MC. serum with CVF or C3b or after depletion of C3b inactivator. The evidence indicates that in the cold reaction only the initial C3 convertase of the alternative parhway is formed and that this enzyme is responsible for the observed C3 consumption.", "contents": "Reversible activation of proactivator (factor B) of the alternative pathway without cleavage of the molecule. The serum of a patient (M.C.) with chronic glomerulonephritis and renal deposits of IgA, C3, and properdin converted C3 on overnight exposure of 0 degrees C. The cold reaction was dependent on immunoglobulin, initiating factor, Factors B and D, and magnesium but not on properdin. Factor B, the C3-cleaving enzyme in this reaction, was used in zymogen form. After participation in this rection, Factor B zymogen in M.C. serum could be fully activated by cobra venom factor (CVF) at 37 degrees C. That activation without fragmentation was not due to an abnormal form of Factor B was shown by its typical cleavage on incubation of MC. serum with CVF or C3b or after depletion of C3b inactivator. The evidence indicates that in the cold reaction only the initial C3 convertase of the alternative parhway is formed and that this enzyme is responsible for the observed C3 consumption.", "PMID": 981969}
{"id": "pubmed23n0032_12825", "title": "PHA-induced activation of suppressor cells in normal human peripheral blood lymphocytes.", "content": "Normal human peripheral blood and tonsil lymphocytes can be stimulated to proliferate by phytohemagglutinin (PHA). When cells cultured with this mitogen for 3 days were transferred fo fresh autologous lymphocytes in fresh medium with PHA, the mitogen response of the fresh lymphocytes was suppressed. The suppression required the presence of viable cells, in that culture supernatants alone were not inhibitory and cell extracts showed only marginal inhibition. Approximately equivalent numbers of previously stimulated cells were required to produce optimal suppression of the PHA response of fresh cells. Cells irradiated after PHA stimulation were as effective as nonirradiated cells is causing suppression. PHA-stimulated cells also inhibited concanavalinA-induced proliferation and a mixed lymphocyte reaction. However, PHA-stimulated cells only partially inhibited the response to pokeweed mitogen. The suppressive effects were fully retained by a nylon-wool-enriched T-cell fraction but not by a B-cell-enriched fraction.", "contents": "PHA-induced activation of suppressor cells in normal human peripheral blood lymphocytes. Normal human peripheral blood and tonsil lymphocytes can be stimulated to proliferate by phytohemagglutinin (PHA). When cells cultured with this mitogen for 3 days were transferred fo fresh autologous lymphocytes in fresh medium with PHA, the mitogen response of the fresh lymphocytes was suppressed. The suppression required the presence of viable cells, in that culture supernatants alone were not inhibitory and cell extracts showed only marginal inhibition. Approximately equivalent numbers of previously stimulated cells were required to produce optimal suppression of the PHA response of fresh cells. Cells irradiated after PHA stimulation were as effective as nonirradiated cells is causing suppression. PHA-stimulated cells also inhibited concanavalinA-induced proliferation and a mixed lymphocyte reaction. However, PHA-stimulated cells only partially inhibited the response to pokeweed mitogen. The suppressive effects were fully retained by a nylon-wool-enriched T-cell fraction but not by a B-cell-enriched fraction.", "PMID": 981970}
{"id": "pubmed23n0032_12826", "title": "Immune complexes in human melanoma: a consequence of deranged immune regulation.", "content": "Circulating immune complexes were detected in 62 individuals with malignant melanoma by precipitation with isolated human C1q and polyclonal rheumatoid factors. In 56 patients the C1q deviation assay showed low to moderate levels of complexes, with increased amounts with advancing stage of disease. Both heavy (greater than 19S) and intermediate (7S to 19S) varieties were present, and complexes containing tumor antigen-antibody or antibody-anti-antibody were identified. Complexes were found in the kidneys of one patient with malignancy and the nephrotic syndrome and in two further patients with melanoma in whom there were no clinical manifestations of nephrosis. Serial determinations in 51 patients showed slow cyclic variations in the levels of complexes and fluctuations in response to therapy. The coexistence of anti-antibodies, immune complex disease, and anergy in melanoma patients may indicate a deranged immune regulation consequent to chronic antigenic stimulation by the tumor.", "contents": "Immune complexes in human melanoma: a consequence of deranged immune regulation. Circulating immune complexes were detected in 62 individuals with malignant melanoma by precipitation with isolated human C1q and polyclonal rheumatoid factors. In 56 patients the C1q deviation assay showed low to moderate levels of complexes, with increased amounts with advancing stage of disease. Both heavy (greater than 19S) and intermediate (7S to 19S) varieties were present, and complexes containing tumor antigen-antibody or antibody-anti-antibody were identified. Complexes were found in the kidneys of one patient with malignancy and the nephrotic syndrome and in two further patients with melanoma in whom there were no clinical manifestations of nephrosis. Serial determinations in 51 patients showed slow cyclic variations in the levels of complexes and fluctuations in response to therapy. The coexistence of anti-antibodies, immune complex disease, and anergy in melanoma patients may indicate a deranged immune regulation consequent to chronic antigenic stimulation by the tumor.", "PMID": 981971}
{"id": "pubmed23n0032_12827", "title": "[Comparative study of long-term mortality in the mentally ill].", "content": "The mortality in mental illness has been studied after an average follow-up period of 3-4 decades in a material of 5661 former in-patients of the Psychiatric University Hospital of Lausanne (Switzerland), from which 3520 probands were dead at the date of reference. By use of an improved method, the calculations showed in all diagnostic groups, and generally more marked in women than in men, a 1,34 to 9,72 times higher mortality than in the general population, increasing in the following order: head injuries, psychogenic and psychopathic disorders, manic syndromes, schizophrenia, depressions, mental retardation, alcoholisme, other psychosis, epilepsy, general paralysis, other cerebro-organic conditions. Several possible explanations of these results are discussed.", "contents": "[Comparative study of long-term mortality in the mentally ill]. The mortality in mental illness has been studied after an average follow-up period of 3-4 decades in a material of 5661 former in-patients of the Psychiatric University Hospital of Lausanne (Switzerland), from which 3520 probands were dead at the date of reference. By use of an improved method, the calculations showed in all diagnostic groups, and generally more marked in women than in men, a 1,34 to 9,72 times higher mortality than in the general population, increasing in the following order: head injuries, psychogenic and psychopathic disorders, manic syndromes, schizophrenia, depressions, mental retardation, alcoholisme, other psychosis, epilepsy, general paralysis, other cerebro-organic conditions. Several possible explanations of these results are discussed.", "PMID": 981972}
{"id": "pubmed23n0032_12828", "title": "[The problem of the linguistic and scientivic position of psychopathology in the framework of current psychiatry].", "content": "An attempt has been made to explain on the one hand the significance of the psychopathological terminology. In contrast to the \"dynamic psychiatry\", where the language has preponderantly an anticipating, finalizing character with regard to the therapeutical process, it is here being made clear that the cognition of the full existence requires a defined analysis in case of a psychic distrubance. A conceivable aspiration for orderliness thus opens the way to impartial objectiveness. To a special extent the psychiatrist here appears to be obliged to the word itself, particularly within the framework of transcultural psychiatric research, that is, both in regard to philosophical and cultural-historical conditions. The figurative and symbolic character of the language seems to be necessary in psychopathology to make clear the existence of a psychopathological state of mind for lack of another system of definition. On the other hand, it should be shown that also psychopathology - without being subjected to foreign infiltration of a philosophysing medical doctrine - must be embedded in the philosophical way of thinking, if it will not evade an answer to the all important question of a finiteness or infiniteness of man beyond all positive ways of thinking by the fact of the psychophysical problems amid its science and the unique irrationality thereof.", "contents": "[The problem of the linguistic and scientivic position of psychopathology in the framework of current psychiatry]. An attempt has been made to explain on the one hand the significance of the psychopathological terminology. In contrast to the \"dynamic psychiatry\", where the language has preponderantly an anticipating, finalizing character with regard to the therapeutical process, it is here being made clear that the cognition of the full existence requires a defined analysis in case of a psychic distrubance. A conceivable aspiration for orderliness thus opens the way to impartial objectiveness. To a special extent the psychiatrist here appears to be obliged to the word itself, particularly within the framework of transcultural psychiatric research, that is, both in regard to philosophical and cultural-historical conditions. The figurative and symbolic character of the language seems to be necessary in psychopathology to make clear the existence of a psychopathological state of mind for lack of another system of definition. On the other hand, it should be shown that also psychopathology - without being subjected to foreign infiltration of a philosophysing medical doctrine - must be embedded in the philosophical way of thinking, if it will not evade an answer to the all important question of a finiteness or infiniteness of man beyond all positive ways of thinking by the fact of the psychophysical problems amid its science and the unique irrationality thereof.", "PMID": 981973}
{"id": "pubmed23n0032_12829", "title": "Psychopathometry and homogenous syndrome dynamics.", "content": "The principle of homogeneous dynamic of syndromes means that diminishing of single psychic functions or the severeness of syndromes is correlated to the total syndrome. The validity of this principle was alleged for reversible physically founded psychoses and for cyclothymic depressions. Various empirical investigations confirm the validity of the principle of homogeneous dynamic of syndromes. These findings suggest practical benefit for the development of psychopathometric procedures: constructions of short tests. Perhaps the range of validity of the principle of homogeneous dynamic of syndromes may be extended on cyclothymic mania, too. Empirical investigations will prove this question.", "contents": "Psychopathometry and homogenous syndrome dynamics. The principle of homogeneous dynamic of syndromes means that diminishing of single psychic functions or the severeness of syndromes is correlated to the total syndrome. The validity of this principle was alleged for reversible physically founded psychoses and for cyclothymic depressions. Various empirical investigations confirm the validity of the principle of homogeneous dynamic of syndromes. These findings suggest practical benefit for the development of psychopathometric procedures: constructions of short tests. Perhaps the range of validity of the principle of homogeneous dynamic of syndromes may be extended on cyclothymic mania, too. Empirical investigations will prove this question.", "PMID": 981974}
{"id": "pubmed23n0032_12830", "title": "[Anorexic syndrome and depression. Considerations from a case history].", "content": "A connection between anorexic syndrome and depression has been the object of psychoanalytic as well as psychiatric considerations for a long time now. We report the case of a 45-year-old woman suffering from anorexia nervosa and depression. Biographically-anamnestic aspects are shown in connection with depth-psychological and psychiatric ones, thus trying to achieve a synopsis.", "contents": "[Anorexic syndrome and depression. Considerations from a case history]. A connection between anorexic syndrome and depression has been the object of psychoanalytic as well as psychiatric considerations for a long time now. We report the case of a 45-year-old woman suffering from anorexia nervosa and depression. Biographically-anamnestic aspects are shown in connection with depth-psychological and psychiatric ones, thus trying to achieve a synopsis.", "PMID": 981975}
{"id": "pubmed23n0032_12831", "title": "[Pseudoparalytic myasthenia gravis. Diagnostic and therapeutic aspects in 60 separate cases].", "content": "Based on 60 of our own cases and on the medical literature the authors discuss the diagnostic, pathophysiological and therapeutic aspects of myasthenia gravis. Myasthenia is suspected in cases of motor weakness of changing intensity, diminishing by rest. The weak muscles are innervated by different peripheral nerves. At the beginning a weakness of upperlid-muscles, external eye muscles and bulbar muscles is particularly frequent. There is no sensory loss or other neurological symptoms. A transitory disappearance of motor weakness after an intravenous dose of Edrophonium (Tensilon) is a typical diagnostic sign. The effect is less evident with eye-muscle weakness. A typical appearance of potentials after repetitive stimulation of peripheral nerves as well as other characteristics in electrophysiological testing of muscles are of high diagnostic value. This allows differentiation from other types of muscle weakness. In the pathogenesis of myasthenia an autoimmune process related to a persistent thymus gland plays an important part. This leads to an ultrastructural change in the postsynaptic membrane of the muscle fibre. The postsynaptic membrane no longer reacts in a normal way to acetylcholine as a transmitter substance at the level of the motor endplate. Therefore the first step in the treatment of myasthenia consists of cholinesterase-inhibitors, specially Neostigmin (Prostigmin) and Pyridostigmin (Mestinon). Thymectomy is advised in all cases of myasthenia with the exception of the pure ocular form and of myasthenia in patients older than 60 years. The thymus gland is practically always persistent or hypertrophic in myasthenia. The suprasternal access is recommended. A thymoma should always be operated upon because of the danger of malignancy. In cases where thymectomy is not performed or not successful and if cholinesterase-inhibitors are not sufficiently efficient, treatment with corticosteroids or ACTH is recommended.", "contents": "[Pseudoparalytic myasthenia gravis. Diagnostic and therapeutic aspects in 60 separate cases]. Based on 60 of our own cases and on the medical literature the authors discuss the diagnostic, pathophysiological and therapeutic aspects of myasthenia gravis. Myasthenia is suspected in cases of motor weakness of changing intensity, diminishing by rest. The weak muscles are innervated by different peripheral nerves. At the beginning a weakness of upperlid-muscles, external eye muscles and bulbar muscles is particularly frequent. There is no sensory loss or other neurological symptoms. A transitory disappearance of motor weakness after an intravenous dose of Edrophonium (Tensilon) is a typical diagnostic sign. The effect is less evident with eye-muscle weakness. A typical appearance of potentials after repetitive stimulation of peripheral nerves as well as other characteristics in electrophysiological testing of muscles are of high diagnostic value. This allows differentiation from other types of muscle weakness. In the pathogenesis of myasthenia an autoimmune process related to a persistent thymus gland plays an important part. This leads to an ultrastructural change in the postsynaptic membrane of the muscle fibre. The postsynaptic membrane no longer reacts in a normal way to acetylcholine as a transmitter substance at the level of the motor endplate. Therefore the first step in the treatment of myasthenia consists of cholinesterase-inhibitors, specially Neostigmin (Prostigmin) and Pyridostigmin (Mestinon). Thymectomy is advised in all cases of myasthenia with the exception of the pure ocular form and of myasthenia in patients older than 60 years. The thymus gland is practically always persistent or hypertrophic in myasthenia. The suprasternal access is recommended. A thymoma should always be operated upon because of the danger of malignancy. In cases where thymectomy is not performed or not successful and if cholinesterase-inhibitors are not sufficiently efficient, treatment with corticosteroids or ACTH is recommended.", "PMID": 981976}
{"id": "pubmed23n0032_12832", "title": "E1Socio-economic data concerning the early childhood and the actual life-situation of psychiatric out-patients.", "content": "By means of a standarized social psychiatric questionnaire which was developed from the AMP-system and which forms the basis for a standardized case history used in our outpatient clinic, a random sample of 500 ambulant patients from the years 1970 and 1971 were examined concerning their socio-economic data. Patients of the age group of 20-24 years were overrepresented (p less than 0,05) as well as patients of the lower socio-economic classes and patients whose parents were less than 20 years old at the time of the patients' birth. The structure of the family in childhood showed significant differences concerning patients with different social contacts and different diagnoses. Patients who grew up in big families have themselves a relatively large number of children and vice versa (p less than 0,01). A significant correlation (p less than 0,01) consists also between the number of siblings of the patients and the socio-economic class they belong to: Patients from the underprivileged socio-economic classes mostly grew up in big families.", "contents": "E1Socio-economic data concerning the early childhood and the actual life-situation of psychiatric out-patients. By means of a standarized social psychiatric questionnaire which was developed from the AMP-system and which forms the basis for a standardized case history used in our outpatient clinic, a random sample of 500 ambulant patients from the years 1970 and 1971 were examined concerning their socio-economic data. Patients of the age group of 20-24 years were overrepresented (p less than 0,05) as well as patients of the lower socio-economic classes and patients whose parents were less than 20 years old at the time of the patients' birth. The structure of the family in childhood showed significant differences concerning patients with different social contacts and different diagnoses. Patients who grew up in big families have themselves a relatively large number of children and vice versa (p less than 0,01). A significant correlation (p less than 0,01) consists also between the number of siblings of the patients and the socio-economic class they belong to: Patients from the underprivileged socio-economic classes mostly grew up in big families.", "PMID": 981977}
{"id": "pubmed23n0032_12833", "title": "[Drug abuse and suicidal tendencies].", "content": "66 juvenile drug-consumers--development-crises (32%), neurotic developments (30%), abnormal juvenile personalities (38%) - with a predominantly polytoxicomanic drug-anamnesis of several years are examined as to their suicidal development. The quantitative and qualitative progression of drug-abuse, the duration of drug-anamnesis, frequent broken-home-situations, grave educational deficiencies together with lacking possibilities of identification, a distinct social decline and chronic conflict-situations indicate the serious degree of the suicidal development. Different suicidal syndromes are worked out which in the form of death-phantasies or suicidal thoughts and horror trips directly derive from drug-abuse or are accompanied and reinforced by a continuous and frequently increasing drug-consumption, and which extend from an increase in depressive irritations, further psychological disturbances and personality changes, doubts about one's personal value and a sense of guilt to the \"sickness of death\".", "contents": "[Drug abuse and suicidal tendencies]. 66 juvenile drug-consumers--development-crises (32%), neurotic developments (30%), abnormal juvenile personalities (38%) - with a predominantly polytoxicomanic drug-anamnesis of several years are examined as to their suicidal development. The quantitative and qualitative progression of drug-abuse, the duration of drug-anamnesis, frequent broken-home-situations, grave educational deficiencies together with lacking possibilities of identification, a distinct social decline and chronic conflict-situations indicate the serious degree of the suicidal development. Different suicidal syndromes are worked out which in the form of death-phantasies or suicidal thoughts and horror trips directly derive from drug-abuse or are accompanied and reinforced by a continuous and frequently increasing drug-consumption, and which extend from an increase in depressive irritations, further psychological disturbances and personality changes, doubts about one's personal value and a sense of guilt to the \"sickness of death\".", "PMID": 981978}
{"id": "pubmed23n0032_12834", "title": "[Neuropsychological study of an autonomous aged population].", "content": "In order to adjust the tests used in a study on degenerative dementia a group of independent aged volunteers has been formed and submitted to neuropsychological tests on instrumental functioning. Among them: 25% obtained normal results - 55% showed light instrumental deficiencies which were however not significant of brain damage - 22% had a global impairment of instrumental functions but without memory troubles. This relatively high percentage of impaired old people posed the problem of the normality of the sample chosen. As it mostly came from elderly communities it is possible to think that these gather more or less psychically or socially disfavoured aged people. The frequency of cortical impairments urges to think that they should be followed up to determine whether these symptoms are normal or if these are the signs of a degenerative evolution; in which case the neuropsychological approach would no longer be selective.", "contents": "[Neuropsychological study of an autonomous aged population]. In order to adjust the tests used in a study on degenerative dementia a group of independent aged volunteers has been formed and submitted to neuropsychological tests on instrumental functioning. Among them: 25% obtained normal results - 55% showed light instrumental deficiencies which were however not significant of brain damage - 22% had a global impairment of instrumental functions but without memory troubles. This relatively high percentage of impaired old people posed the problem of the normality of the sample chosen. As it mostly came from elderly communities it is possible to think that these gather more or less psychically or socially disfavoured aged people. The frequency of cortical impairments urges to think that they should be followed up to determine whether these symptoms are normal or if these are the signs of a degenerative evolution; in which case the neuropsychological approach would no longer be selective.", "PMID": 981979}
{"id": "pubmed23n0032_12835", "title": "[Comparative study of 2 groups of paranoid syndromes appearing at different ages].", "content": "Clinical study of two groups of females beyond age of 65, institutionalized for delusional manifestations of schizophrenic nature and presenting also, at the time of examination, a pronounced paranoid state. In the first group: the manifestations had arisen before the age of 45. In the second group, after the age of 65. This study has demonstrated certain psychopathological characteristics suggesting the presence of personality problems definitely more profound in patients of the first group: autistic state, asthenia, thought disorder, incoherence and vagueness of delusional subjects, ordinarily much more unreal are characteristics of the first group in comparison to the second. This seems to bring evidence that these two paranoid states (paranoid schizophrenia in adult age and paranoid state in senility) are, at first sight, pathological entities based on personality problems of very different intensity.", "contents": "[Comparative study of 2 groups of paranoid syndromes appearing at different ages]. Clinical study of two groups of females beyond age of 65, institutionalized for delusional manifestations of schizophrenic nature and presenting also, at the time of examination, a pronounced paranoid state. In the first group: the manifestations had arisen before the age of 45. In the second group, after the age of 65. This study has demonstrated certain psychopathological characteristics suggesting the presence of personality problems definitely more profound in patients of the first group: autistic state, asthenia, thought disorder, incoherence and vagueness of delusional subjects, ordinarily much more unreal are characteristics of the first group in comparison to the second. This seems to bring evidence that these two paranoid states (paranoid schizophrenia in adult age and paranoid state in senility) are, at first sight, pathological entities based on personality problems of very different intensity.", "PMID": 981980}
{"id": "pubmed23n0032_12836", "title": "[Malpractice in psychotherapy].", "content": "Two cases of malpractice in psychotherapy are described. In one case there was a psychiatrist involved, in the other a non-medical psychotherapist. The legal aspects of malpractice in the view of Swiss legislation are shortly discussed. A legal liability for damages caused by the psychotherapist might arise if the following conditions are fulfilled: A deviance from the generally accepted standards of care in psychotherapy, a damage to the patient as a consequence of this deviance and a negligence on the part of the psychotherapist. Furthermore, there has to be an adequate causal nexus between malpractice and damage done to the patient. By some case reports the author tries to exemplify what can be understood by the term of generally accepted standards of care in psychotherapy.", "contents": "[Malpractice in psychotherapy]. Two cases of malpractice in psychotherapy are described. In one case there was a psychiatrist involved, in the other a non-medical psychotherapist. The legal aspects of malpractice in the view of Swiss legislation are shortly discussed. A legal liability for damages caused by the psychotherapist might arise if the following conditions are fulfilled: A deviance from the generally accepted standards of care in psychotherapy, a damage to the patient as a consequence of this deviance and a negligence on the part of the psychotherapist. Furthermore, there has to be an adequate causal nexus between malpractice and damage done to the patient. By some case reports the author tries to exemplify what can be understood by the term of generally accepted standards of care in psychotherapy.", "PMID": 981981}
{"id": "pubmed23n0032_12837", "title": "[Differential diagnostic question: traumatic unconsciousness or amnestic episode?].", "content": "Traumatic twilight state on the one and amnestic episode (transient global amnesia) on the other side are as a rule easy to differentiate from the patient's age, his behavior during the acute state of disease and the kind of its improving. Contrary to other authors' opinion, differentiation is not just possible by diagnosing an injury or its absence. In minor skull traumata preceding the traumatic twilight states injuries are often not manifest and otherwise amnestic episodes occasionnally occur after minor traumata. In a 64 years old woman an obviously typical amnestic episode appeared in direct connection with a probable fall on the back of the head. A 28 years old man underwent a five-hour amnestic episode whereas the EEG showed normal curves. The episode occurred 15 minutes after striking hard upon the ground with the back of the head during a ground roll without following unconsciousness. In both cases traumatic twilight states were not involved. In account of the interval of 15 minutes the trauma of the second case seems not be be the direct cause for the manifestation of amnestic episode. It also shows that the aspect of the disease in the rare occurrence in younger or middle-aged persons is on principle not different from that in old ones. Since a special medical examination during the acute state is only possible as exception the patient's amnestically seizable behaviour obtains greatest importance in differential diagnosis. For the rare coincidence the trauma is given no pathogenetic relevance in amnestic episodes.", "contents": "[Differential diagnostic question: traumatic unconsciousness or amnestic episode?]. Traumatic twilight state on the one and amnestic episode (transient global amnesia) on the other side are as a rule easy to differentiate from the patient's age, his behavior during the acute state of disease and the kind of its improving. Contrary to other authors' opinion, differentiation is not just possible by diagnosing an injury or its absence. In minor skull traumata preceding the traumatic twilight states injuries are often not manifest and otherwise amnestic episodes occasionnally occur after minor traumata. In a 64 years old woman an obviously typical amnestic episode appeared in direct connection with a probable fall on the back of the head. A 28 years old man underwent a five-hour amnestic episode whereas the EEG showed normal curves. The episode occurred 15 minutes after striking hard upon the ground with the back of the head during a ground roll without following unconsciousness. In both cases traumatic twilight states were not involved. In account of the interval of 15 minutes the trauma of the second case seems not be be the direct cause for the manifestation of amnestic episode. It also shows that the aspect of the disease in the rare occurrence in younger or middle-aged persons is on principle not different from that in old ones. Since a special medical examination during the acute state is only possible as exception the patient's amnestically seizable behaviour obtains greatest importance in differential diagnosis. For the rare coincidence the trauma is given no pathogenetic relevance in amnestic episodes.", "PMID": 981982}
{"id": "pubmed23n0032_12838", "title": "The clinical classification of ceroid-lipofuscinosis. A statistical approach.", "content": "A revision of 108 published cases from the last ten years has been made, of patients suffering ceroid-lipofuscinosis, which includes two cases studied in our department. The statistical analysis of the initial symptoms, the age of onset and the disease's duration, permits us to conclude that possibly there exists, within this syndrome, four different forms in accordance with the age of onset. Each one of them has a typical clinical pattern. Our classification was compared with previously existing ones.", "contents": "The clinical classification of ceroid-lipofuscinosis. A statistical approach. A revision of 108 published cases from the last ten years has been made, of patients suffering ceroid-lipofuscinosis, which includes two cases studied in our department. The statistical analysis of the initial symptoms, the age of onset and the disease's duration, permits us to conclude that possibly there exists, within this syndrome, four different forms in accordance with the age of onset. Each one of them has a typical clinical pattern. Our classification was compared with previously existing ones.", "PMID": 981983}
{"id": "pubmed23n0032_12839", "title": "[Etiology and clinic of status epilepticus].", "content": "In the presented study we examined 110 patients who suffered from status epilepticus. Total 148 status were analysed with regard to etiology, releasing factors, course and treatment. Causes stressed were head injury with brain damage and intracranial space occupying, especially in the frontal region. Head injury with opened dura mater showed more tendency for development of status than closed ones. In a quarter of all cases  origin couldn't be found. All these, with exception of one case, developed intercurrently. Isolated or initial always was consequence of brain lesion, the origin of which was or has been detected. No trend with reference to distribution of age in the particular forms of status were found out. In a third part of all a releasing factor could be discovered. Most the question in these cases was failure in medicamental treatment. We couldn't draw one's interferences about pathogenesis from etiology, age of patient, place in course and form of status. About in a quarter of all status we found defective neurological sequels, in 12% also psychical disturbances. 16 patients died during the period of observation, but only 2 within 24 hours, all together 8 in the first 14 days in deap coma. Immediately cause of death were above all complications of the heart, circulation of blood and respiration tract. In the most cases the treatment consisted in a mixture of anticonvulsive medicaments. The predominant rate of status epilepticus could be inhibited within 24 hours, only 20 lasted a longer time.", "contents": "[Etiology and clinic of status epilepticus]. In the presented study we examined 110 patients who suffered from status epilepticus. Total 148 status were analysed with regard to etiology, releasing factors, course and treatment. Causes stressed were head injury with brain damage and intracranial space occupying, especially in the frontal region. Head injury with opened dura mater showed more tendency for development of status than closed ones. In a quarter of all cases  origin couldn't be found. All these, with exception of one case, developed intercurrently. Isolated or initial always was consequence of brain lesion, the origin of which was or has been detected. No trend with reference to distribution of age in the particular forms of status were found out. In a third part of all a releasing factor could be discovered. Most the question in these cases was failure in medicamental treatment. We couldn't draw one's interferences about pathogenesis from etiology, age of patient, place in course and form of status. About in a quarter of all status we found defective neurological sequels, in 12% also psychical disturbances. 16 patients died during the period of observation, but only 2 within 24 hours, all together 8 in the first 14 days in deap coma. Immediately cause of death were above all complications of the heart, circulation of blood and respiration tract. In the most cases the treatment consisted in a mixture of anticonvulsive medicaments. The predominant rate of status epilepticus could be inhibited within 24 hours, only 20 lasted a longer time.", "PMID": 981984}
{"id": "pubmed23n0032_12840", "title": "Narcolepsy and hypersomnia: review and classification of 642 personally observed cases.", "content": "In this paper the author gives a survey and a classification of 642 cases of narcolepsy and hypersomnia which he himself studied in the course of 26 years. 368 cases were classified as narcolepsy, 274 as hypersomnia. The author further classifies narcolepsies according to their etiology, clinical form and pathophysiological mechanisms of origin. Hypersomnias are divided by the author into the symptomatic and the functional groups. According to the author it is useful to distinguish \"short cycle hypersomnia\", i.e. those with short duration of sleep attacks (hours) and intervals, from \"long cycle hypersomnia\", i.e. those with long attacks (days or weeks) and intervals. The author goes on to describe different forms of symptomatic and functional hypersomnias, such as idiopathic hypersomnia, neurotic hypersomnia, the Pickwickian syndrome\" and its variants as well as different varieties of periodic long cycle hypersomnias. Finally the author makes a brief mention of the syndrome of insufficiency of wakefulness.", "contents": "Narcolepsy and hypersomnia: review and classification of 642 personally observed cases. In this paper the author gives a survey and a classification of 642 cases of narcolepsy and hypersomnia which he himself studied in the course of 26 years. 368 cases were classified as narcolepsy, 274 as hypersomnia. The author further classifies narcolepsies according to their etiology, clinical form and pathophysiological mechanisms of origin. Hypersomnias are divided by the author into the symptomatic and the functional groups. According to the author it is useful to distinguish \"short cycle hypersomnia\", i.e. those with short duration of sleep attacks (hours) and intervals, from \"long cycle hypersomnia\", i.e. those with long attacks (days or weeks) and intervals. The author goes on to describe different forms of symptomatic and functional hypersomnias, such as idiopathic hypersomnia, neurotic hypersomnia, the Pickwickian syndrome\" and its variants as well as different varieties of periodic long cycle hypersomnias. Finally the author makes a brief mention of the syndrome of insufficiency of wakefulness.", "PMID": 981985}
{"id": "pubmed23n0032_12841", "title": "[Modern images of womanhood and their relationship to fetishism].", "content": "The image of womanhood accepted by society has changed from the corseted lady at the beginning of the 20th century to the emancipated and booted woman of today. The dictates of fashion in clothing correspond to unconscious wishes nurtured by men; the present fashion enables latent fetishist tendencies to work themselves out within the social framework and is directed at creating the \"phallic woman\". The boot and leather fetishism masquerading under the guise of a fashionable trend sheds light on unconscious sexual behaviour in the male, the underlying psychodynamics of which are further illuminated by the -for the moment, at least- still \"private\" model of rubber fetishism. Similar arguments apply to the current wave of transvestism and pseudo-transvestism. An analysis of certain comic strips and television serials renders such tendencies even more clearly apparent. Both decorative art and the pop show business are showing signs of a trend towards an exchange of roles between the sexes; and ambisexual image valid for both sexes is now emerging.", "contents": "[Modern images of womanhood and their relationship to fetishism]. The image of womanhood accepted by society has changed from the corseted lady at the beginning of the 20th century to the emancipated and booted woman of today. The dictates of fashion in clothing correspond to unconscious wishes nurtured by men; the present fashion enables latent fetishist tendencies to work themselves out within the social framework and is directed at creating the \"phallic woman\". The boot and leather fetishism masquerading under the guise of a fashionable trend sheds light on unconscious sexual behaviour in the male, the underlying psychodynamics of which are further illuminated by the -for the moment, at least- still \"private\" model of rubber fetishism. Similar arguments apply to the current wave of transvestism and pseudo-transvestism. An analysis of certain comic strips and television serials renders such tendencies even more clearly apparent. Both decorative art and the pop show business are showing signs of a trend towards an exchange of roles between the sexes; and ambisexual image valid for both sexes is now emerging.", "PMID": 981986}
{"id": "pubmed23n0032_12842", "title": "[The psychopathology of early and chronic psychotic symptoms in young drug-users].", "content": "Among the psychotic symptoms in juvenile drug-consumers one can find autonomous, i.e. drug-independent developments, whose connection with the drug-abuse is to be assessed in differing ways. A beginning psychosis can be modified in its actual symptoms by drug-consumption. On the other hand one must consider the manifestation of a latent psychosis or purely symptomatic psychosis, which, in its symptoms, can hardly be distinguished from schizophrenia. Finally drug-induced personality-changes can develop together with secondary psychotic symptoms. Psychotic symptoms are determined and influenced in a varying degree by drugs. Both after short drug-consumption and after a longer drug-anamnesis with polytoxicomanic symptoms psychotic syndromes can be discovered. Even the initial psychotic symptoms can hint at an adverse development and a bad prognosis. Sometimes the drug-experiences conceal the autonomous development of the psychosis, which, as a rule, shows predominantly schizophrenic symptoms. In addition to a quick change of the actual symptoms, acute states of confusion and depressive-suicidal syndromes, flash-back and horror-trip phenomena, closely connected with the psychotic experience, and a schizophrenic colouring of affective psychoses can be found as frequently drug-induced modifications of the psychotic symptoms. Furthermore one finds an increase of symptoms and of the psychotic episodes in the case of psychoses of the schizophrenic variety which have already begun. Grave personality changes with psychotic symptoms after chronic drug-abuse can cause differential-diagnostic difficulties.", "contents": "[The psychopathology of early and chronic psychotic symptoms in young drug-users]. Among the psychotic symptoms in juvenile drug-consumers one can find autonomous, i.e. drug-independent developments, whose connection with the drug-abuse is to be assessed in differing ways. A beginning psychosis can be modified in its actual symptoms by drug-consumption. On the other hand one must consider the manifestation of a latent psychosis or purely symptomatic psychosis, which, in its symptoms, can hardly be distinguished from schizophrenia. Finally drug-induced personality-changes can develop together with secondary psychotic symptoms. Psychotic symptoms are determined and influenced in a varying degree by drugs. Both after short drug-consumption and after a longer drug-anamnesis with polytoxicomanic symptoms psychotic syndromes can be discovered. Even the initial psychotic symptoms can hint at an adverse development and a bad prognosis. Sometimes the drug-experiences conceal the autonomous development of the psychosis, which, as a rule, shows predominantly schizophrenic symptoms. In addition to a quick change of the actual symptoms, acute states of confusion and depressive-suicidal syndromes, flash-back and horror-trip phenomena, closely connected with the psychotic experience, and a schizophrenic colouring of affective psychoses can be found as frequently drug-induced modifications of the psychotic symptoms. Furthermore one finds an increase of symptoms and of the psychotic episodes in the case of psychoses of the schizophrenic variety which have already begun. Grave personality changes with psychotic symptoms after chronic drug-abuse can cause differential-diagnostic difficulties.", "PMID": 981987}
{"id": "pubmed23n0032_12843", "title": "[Necrophilia in a 17 year old girl].", "content": "Case report of true necrophilia in a seventeen-year-old girl, which is the first description of such a behaviour in a woman. The possible development of the abnormal behaviour is discussed. A complete theory of necrophilia semms to be not yet possible because of the few cases described.", "contents": "[Necrophilia in a 17 year old girl]. Case report of true necrophilia in a seventeen-year-old girl, which is the first description of such a behaviour in a woman. The possible development of the abnormal behaviour is discussed. A complete theory of necrophilia semms to be not yet possible because of the few cases described.", "PMID": 981988}
{"id": "pubmed23n0032_12844", "title": "Ultrastructural changes in cartilage after intra-articular administration of osmium tetroxide and the sodium salts of fish oil fatty acids (Varicocid).", "content": "A clinical and experimental study was carried out on knee joints of rabbits and in humans. In one knee joint of each rabbit 0.3 ml 1% osmic acid or 0.3 ml 5% Varicocid was injected, the remaining uninjected joints serving as controls. The animals were killed after 1 and 24 hours, 15, 45 and 90 days. Study with the electron microscope after osmic acid injection revealed necrosis of the chondrocytes in the superficial layer and upper middle zone. The articulations injected with Varicocid exhibited only slight lesions of the chondrocytes in the upper layer, consisting of dilated rough endoplasmatic reticulum, mitochondria lacking matrix and christae; the electron density of the layer seemed to be diminished. Biopsy of the non-weight-bearing cartilage of the lateral femoral condyle was performed in 4 patients with Rheumatoid Arthritis and arthritis of the knee in the onset stage. Two of these patients received one intra-articular injection of 10 ml 1% osmic acid, 5 ml 2% xylocaine and 50 mg hydrocortisone and the other 2 patients 6 ml 5% Varicocid. Biopsy of the cartilage in the areas adjacent to the site of the first biopsies 1 and 3 1/2 months after treatment with osmic acid and 2 and 3 1/2 months after treatment with Varicocid showed in the first two cases necrosis of the chondrocytes in the superficial layer and upper middle zone of the articular cartilage and depletion of the mucopolysaccharides in the ground substance, revealed by PAS and alcian blue staining. No lesion of the knee joint cartilage was observed in the other two cases. In view of the results obtained, chemical synovectomy with Varicocid can be considered as having received further support.", "contents": "Ultrastructural changes in cartilage after intra-articular administration of osmium tetroxide and the sodium salts of fish oil fatty acids (Varicocid). A clinical and experimental study was carried out on knee joints of rabbits and in humans. In one knee joint of each rabbit 0.3 ml 1% osmic acid or 0.3 ml 5% Varicocid was injected, the remaining uninjected joints serving as controls. The animals were killed after 1 and 24 hours, 15, 45 and 90 days. Study with the electron microscope after osmic acid injection revealed necrosis of the chondrocytes in the superficial layer and upper middle zone. The articulations injected with Varicocid exhibited only slight lesions of the chondrocytes in the upper layer, consisting of dilated rough endoplasmatic reticulum, mitochondria lacking matrix and christae; the electron density of the layer seemed to be diminished. Biopsy of the non-weight-bearing cartilage of the lateral femoral condyle was performed in 4 patients with Rheumatoid Arthritis and arthritis of the knee in the onset stage. Two of these patients received one intra-articular injection of 10 ml 1% osmic acid, 5 ml 2% xylocaine and 50 mg hydrocortisone and the other 2 patients 6 ml 5% Varicocid. Biopsy of the cartilage in the areas adjacent to the site of the first biopsies 1 and 3 1/2 months after treatment with osmic acid and 2 and 3 1/2 months after treatment with Varicocid showed in the first two cases necrosis of the chondrocytes in the superficial layer and upper middle zone of the articular cartilage and depletion of the mucopolysaccharides in the ground substance, revealed by PAS and alcian blue staining. No lesion of the knee joint cartilage was observed in the other two cases. In view of the results obtained, chemical synovectomy with Varicocid can be considered as having received further support.", "PMID": 981989}
{"id": "pubmed23n0032_12845", "title": "The effect of non-specifically acting transfer factor component on cellular immunity in juvenile rheumatoid arthritis.", "content": "A chromatographically purified component of human dialysable transfer factor with a non-specific stimulatory effect on the expression of immune response was used in a therapeutic trial in 8 cases of juvenile rheumatoid arthritis. Enhancement of the delayed type of immune response, measured by skin testing, was seen in all cases, but in vitro reactions to test antigens were unaltered. Clinical improvement was seen in all patients with acute history and in 2 of the 5 chronic cases, but it is not certain whether this was caused by the improved immune reactivity, or whether this reflected the known variable natural history of the disease.", "contents": "The effect of non-specifically acting transfer factor component on cellular immunity in juvenile rheumatoid arthritis. A chromatographically purified component of human dialysable transfer factor with a non-specific stimulatory effect on the expression of immune response was used in a therapeutic trial in 8 cases of juvenile rheumatoid arthritis. Enhancement of the delayed type of immune response, measured by skin testing, was seen in all cases, but in vitro reactions to test antigens were unaltered. Clinical improvement was seen in all patients with acute history and in 2 of the 5 chronic cases, but it is not certain whether this was caused by the improved immune reactivity, or whether this reflected the known variable natural history of the disease.", "PMID": 981991}
{"id": "pubmed23n0032_12846", "title": "Chloroquine retinopathy in patients with rheumatoid arthritis.", "content": "270 consecutive patients with rheumatoid arthritis who had received chloroquine therapy were examined ophthalmologically for toxic retinal lesions. The total annual dose of chloroquine was 70-75 g. The maximum total dose given was 1330 g. The duration of treatment ranged up to 15 years. The primary material was divided into four groups according to total chloroquine dose received: greater than 100 g, 101-300 g, 301-600 g, and less than 600 g. Each dose group was arbitrarily split into two age groups, one with patients under 63 years of age and the other with patients over 63, in order to analyse the effect of age upon the ocular findings. In the present study, slight macular changes were included in the concept of maculopathy and were not thought to contra-indicate chloroquine therapy. Macular changes were found in about 25% of the patients, regardless of age in the lowest dosage group. The frequency of maculopathy increased with increasing total dose only in the older age group. It was also shown that the frequency of maculopathies and other eye diseases also increased with increasing age. This was evident even from the age of 50. The only patient with chloroquine retinopathy was an inadequately controlled 74-year-old woman. Chloroquine treatment of rheumatoid arthritis in the absence of any other disease which may cause retinopathy implies negligible risks in adult patients under 50 years of age. These patients could be less frequently checked. Older patients require regular ophthalmological checks. It is important to use the smallest effective dose possible, and never higher than 4 mg of chloroquine phosphate/kg body weight and day for 10 months annually; in elderly patients, preferably even lower doses.", "contents": "Chloroquine retinopathy in patients with rheumatoid arthritis. 270 consecutive patients with rheumatoid arthritis who had received chloroquine therapy were examined ophthalmologically for toxic retinal lesions. The total annual dose of chloroquine was 70-75 g. The maximum total dose given was 1330 g. The duration of treatment ranged up to 15 years. The primary material was divided into four groups according to total chloroquine dose received: greater than 100 g, 101-300 g, 301-600 g, and less than 600 g. Each dose group was arbitrarily split into two age groups, one with patients under 63 years of age and the other with patients over 63, in order to analyse the effect of age upon the ocular findings. In the present study, slight macular changes were included in the concept of maculopathy and were not thought to contra-indicate chloroquine therapy. Macular changes were found in about 25% of the patients, regardless of age in the lowest dosage group. The frequency of maculopathy increased with increasing total dose only in the older age group. It was also shown that the frequency of maculopathies and other eye diseases also increased with increasing age. This was evident even from the age of 50. The only patient with chloroquine retinopathy was an inadequately controlled 74-year-old woman. Chloroquine treatment of rheumatoid arthritis in the absence of any other disease which may cause retinopathy implies negligible risks in adult patients under 50 years of age. These patients could be less frequently checked. Older patients require regular ophthalmological checks. It is important to use the smallest effective dose possible, and never higher than 4 mg of chloroquine phosphate/kg body weight and day for 10 months annually; in elderly patients, preferably even lower doses.", "PMID": 981992}
{"id": "pubmed23n0032_12847", "title": "Follow-up study of synovectomies of the knee joint in patients suffering from rheumatoid arthritis.", "content": "Synovectomies of the knee joint that were performed during the years 1960-65 at the Rheumatism Foundation Hospital because of rheumatoid arthritis have been re-examined after the lapse of 9.5 years in 156 patients and 202 knee joints and 110 non-operated knee joints of the same patients. Track of motion was helped both in synovectomized and in control knees. Deficiency of extension decreased only in the synovectomized knees; track of motion of the knee was improved by the operation. Swelling and pain appeared less often in operated than in control knees. The patient's own assessment of the operating results was good in 71% and poor in 12% of the cases. Radiological deterioration continued during the follow-up period both in the operated and in the control knees, though it was more severe in the latter. There is a distinct negative correlation between severity of disease and the patients's ability to work. Within the limits of this survey it is not possible to prove that synovectomy halts the radiological destruction of the knee joint; it is mainly a question of a measure that retards the rate of deterioration. One of the chief advantages of synovectomy of the knee is that it helps the patient to live a normal life and makes it possible for him to participate in working life.", "contents": "Follow-up study of synovectomies of the knee joint in patients suffering from rheumatoid arthritis. Synovectomies of the knee joint that were performed during the years 1960-65 at the Rheumatism Foundation Hospital because of rheumatoid arthritis have been re-examined after the lapse of 9.5 years in 156 patients and 202 knee joints and 110 non-operated knee joints of the same patients. Track of motion was helped both in synovectomized and in control knees. Deficiency of extension decreased only in the synovectomized knees; track of motion of the knee was improved by the operation. Swelling and pain appeared less often in operated than in control knees. The patient's own assessment of the operating results was good in 71% and poor in 12% of the cases. Radiological deterioration continued during the follow-up period both in the operated and in the control knees, though it was more severe in the latter. There is a distinct negative correlation between severity of disease and the patients's ability to work. Within the limits of this survey it is not possible to prove that synovectomy halts the radiological destruction of the knee joint; it is mainly a question of a measure that retards the rate of deterioration. One of the chief advantages of synovectomy of the knee is that it helps the patient to live a normal life and makes it possible for him to participate in working life.", "PMID": 981993}
{"id": "pubmed23n0032_12848", "title": "Post-yersinotic Reiter's disease in a physician treated with osmium tetroxide.", "content": "A case of general yersinosis in a male physician is described. Most probably the infection was acquired nosocomially in a paediatric ward. The infection affected several organs including the liver, but osmium tetroxide was nevertheless used to perform chemical synovectomy in a knee joint suffering from active arthritis. The arthritis was cured, and the already existing liver affection did not progress. The only complaint from the patient, months after the osmic acid injection, was a strong metallic taste on the tongue. More than a year after the intra-articular injection, the metallic taste disappeared. The amount of osmium in the patient's blood was then calculated to be less than 100 molecules of osmium per ml blood.", "contents": "Post-yersinotic Reiter's disease in a physician treated with osmium tetroxide. A case of general yersinosis in a male physician is described. Most probably the infection was acquired nosocomially in a paediatric ward. The infection affected several organs including the liver, but osmium tetroxide was nevertheless used to perform chemical synovectomy in a knee joint suffering from active arthritis. The arthritis was cured, and the already existing liver affection did not progress. The only complaint from the patient, months after the osmic acid injection, was a strong metallic taste on the tongue. More than a year after the intra-articular injection, the metallic taste disappeared. The amount of osmium in the patient's blood was then calculated to be less than 100 molecules of osmium per ml blood.", "PMID": 981994}
{"id": "pubmed23n0032_12849", "title": "Late synovectomy of the hand in rheumatoid arthritis.", "content": "PIP joints, MCP joints and wrists of 80 patients with rheumatoid arthritis were operated on with late synovectomy. The rate of recurrence of local synovitis was about 5%, which contrasted favourably with a considerably higher rate of progression of bony erosions. The loss of range of movement was small to moderate. Pain was alleviated in most cases. The possibility of forecasting the results by preoperative parameters was limited. It was concluded that the main indication for late synovectomy of the hand was alleviation of pain. The prophylactic effect on joint destruction seemed to be both slight and unpredictable.", "contents": "Late synovectomy of the hand in rheumatoid arthritis. PIP joints, MCP joints and wrists of 80 patients with rheumatoid arthritis were operated on with late synovectomy. The rate of recurrence of local synovitis was about 5%, which contrasted favourably with a considerably higher rate of progression of bony erosions. The loss of range of movement was small to moderate. Pain was alleviated in most cases. The possibility of forecasting the results by preoperative parameters was limited. It was concluded that the main indication for late synovectomy of the hand was alleviation of pain. The prophylactic effect on joint destruction seemed to be both slight and unpredictable.", "PMID": 981995}
{"id": "pubmed23n0032_12850", "title": "The significance of chemotherapy for relapses from respiratory tuberculosis.", "content": "Relapses were analysed among 1,000 patients with arrested pulmonary tuberculosis who lived in the suburban area of Copenhagen. They have been carefully followed through a period of up till 15 years. Relapses did not occur if the patients had had a full course of modern chemotherapy. Therefore, it is recommended that routine follow-up of these patients be abolished. But relapses were very frequent among patients who had received incomplete chemotherapy or no chemotherapy at all. Attempts have been made to identify the groups particularly prone to a relapse. Age, and the time interval since the previous disease were of importance. Otherwise, neither the character of the disease, the extent of X-ray lesions, the frequency of cavities, nor the conditions at time of cure seemed of significance. Therefore close attention should be paid to all previous tuberculosis patients who have not undergone adequate modern drug treatment and, in selected cases, to consider preventive chemotherapy.", "contents": "The significance of chemotherapy for relapses from respiratory tuberculosis. Relapses were analysed among 1,000 patients with arrested pulmonary tuberculosis who lived in the suburban area of Copenhagen. They have been carefully followed through a period of up till 15 years. Relapses did not occur if the patients had had a full course of modern chemotherapy. Therefore, it is recommended that routine follow-up of these patients be abolished. But relapses were very frequent among patients who had received incomplete chemotherapy or no chemotherapy at all. Attempts have been made to identify the groups particularly prone to a relapse. Age, and the time interval since the previous disease were of importance. Otherwise, neither the character of the disease, the extent of X-ray lesions, the frequency of cavities, nor the conditions at time of cure seemed of significance. Therefore close attention should be paid to all previous tuberculosis patients who have not undergone adequate modern drug treatment and, in selected cases, to consider preventive chemotherapy.", "PMID": 981998}
{"id": "pubmed23n0032_12851", "title": "The effect of voluntary hyperventilation on arterial blood gases and gas exchange in patients with chronic obstructive lung disease.", "content": "A 2 min test of voluntary hyperventilation (VHV) was performed on 19 patients with chronic obstructive lung disease (COLD) and on eight control subjects. The average decrease in PaCO2 in the patients in this test was 4.6 mmHg (11%) and the average increase in PaO2 was 12.3 mmHg (19.1%). The average decrease of PaCO2 in control subjects was 16.5 mmHg (48.6%) and the average increase in PaO2 was 38.0 mmHg (46.2%). In patients with COLD, PaCO2 changes during VHV correlated significantly with respiratory exchange ratio; the correlation with minute and alveolar ventilation and deltaVO2/deltaVE ratio during VHV was of borderline significance. PaCO2 changes during VHV did not correlate with FEV1 (percent of predicted) or FEV1/VC%. The possible mechanisms of changes in blood gas tensions during and after the end of VHV test are discussed. The VHV test seems to be useful in evaluation of patients with COLD.", "contents": "The effect of voluntary hyperventilation on arterial blood gases and gas exchange in patients with chronic obstructive lung disease. A 2 min test of voluntary hyperventilation (VHV) was performed on 19 patients with chronic obstructive lung disease (COLD) and on eight control subjects. The average decrease in PaCO2 in the patients in this test was 4.6 mmHg (11%) and the average increase in PaO2 was 12.3 mmHg (19.1%). The average decrease of PaCO2 in control subjects was 16.5 mmHg (48.6%) and the average increase in PaO2 was 38.0 mmHg (46.2%). In patients with COLD, PaCO2 changes during VHV correlated significantly with respiratory exchange ratio; the correlation with minute and alveolar ventilation and deltaVO2/deltaVE ratio during VHV was of borderline significance. PaCO2 changes during VHV did not correlate with FEV1 (percent of predicted) or FEV1/VC%. The possible mechanisms of changes in blood gas tensions during and after the end of VHV test are discussed. The VHV test seems to be useful in evaluation of patients with COLD.", "PMID": 981999}
{"id": "pubmed23n0032_12852", "title": "Static mechanical lung properties in healthy children.", "content": "The elastic properties of the lung (elastic recoil and static compliance) have been studied in 27 children from 7 to 15 years of age by a quasi-static method. There is a significant relationship between the static expiratory compliance and the quasistatic pressures at different percentages of total lung capacity on the one hand, and height and age on the other. The specific compliance is independent of both height and age. The authors also use a sigmoid mathematical model based on the relationship between specific compliance and maximum pulmonary volume. This model fits well with the experimental data. Comparison is made with the currently used exponential model of Salazar & Knowles. The results favour the hypothesis of an increase in the number of alveoli all through childhood.", "contents": "Static mechanical lung properties in healthy children. The elastic properties of the lung (elastic recoil and static compliance) have been studied in 27 children from 7 to 15 years of age by a quasi-static method. There is a significant relationship between the static expiratory compliance and the quasistatic pressures at different percentages of total lung capacity on the one hand, and height and age on the other. The specific compliance is independent of both height and age. The authors also use a sigmoid mathematical model based on the relationship between specific compliance and maximum pulmonary volume. This model fits well with the experimental data. Comparison is made with the currently used exponential model of Salazar & Knowles. The results favour the hypothesis of an increase in the number of alveoli all through childhood.", "PMID": 982000}
{"id": "pubmed23n0032_12853", "title": "Scanning electron microscopy of the bronchial mucosa in some lung diseases using bronchoscopy specimens. A pilot study including cases of bronchial carcinoma, sarcoidosis, silicosis and tuberculosis.", "content": "In order to study the lung defence mechanisms and the mucociliary transport system scanning electron microscopy was performed on biopsy specimens obtained at bronchoscopy under local anaesthesia. There were great variations in the appearance of the cilitated epithelium from the large bronchi in 40 patients with bronchial carcinoma, sarcoidosis, silicosis, tuberculosis and other lung diseases. Areas with a low frequency of cilia were observed mainly in granulomatous disorders, and in silicosis, atypical cilia occurred abundantly. Squamous metaplasia with a complete loss of cilia was found in chronic bronchitis, bronchial carcinoma, after radiotherapy and in two of seven cases of sarcoidosis. Scanning electron microscopy seems to be an excellent method for studying the surface of the bronchial epithelium in many lung diseases, and may prove to be helpful in occupational medicine.", "contents": "Scanning electron microscopy of the bronchial mucosa in some lung diseases using bronchoscopy specimens. A pilot study including cases of bronchial carcinoma, sarcoidosis, silicosis and tuberculosis. In order to study the lung defence mechanisms and the mucociliary transport system scanning electron microscopy was performed on biopsy specimens obtained at bronchoscopy under local anaesthesia. There were great variations in the appearance of the cilitated epithelium from the large bronchi in 40 patients with bronchial carcinoma, sarcoidosis, silicosis, tuberculosis and other lung diseases. Areas with a low frequency of cilia were observed mainly in granulomatous disorders, and in silicosis, atypical cilia occurred abundantly. Squamous metaplasia with a complete loss of cilia was found in chronic bronchitis, bronchial carcinoma, after radiotherapy and in two of seven cases of sarcoidosis. Scanning electron microscopy seems to be an excellent method for studying the surface of the bronchial epithelium in many lung diseases, and may prove to be helpful in occupational medicine.", "PMID": 982001}
{"id": "pubmed23n0032_12854", "title": "Acute renal failure subsequent to the administration of rifampicin. A follow-up study of cases reported earlier.", "content": "A clinical presentation is made of a 2-3 year follow-up of six cases of acute renal failure that have been reported earlier. The patients had developed transient renal failure after the intermittent administration of rifampicin. The stage of olig-anuria lasted for 1-3 weeks, and five of the patients were treated by hemodialysis. Two of the patients died due to unrelated causes during the follow-up period. The four patients re-examined were clinically cured. Pathologic findings by light microscopy and immunofluorescence at biopsy were scarce. Nothing abnormal was seen by electron microscopy in two of the cases studied. Renal function was normal. In three cases the excretion at 131I-hippuran renography was slightly slowed. Although in the acute stage the renal lesions histologically appeared toxic, evidence suggestive of an immunological mechanism cannot be excluded.", "contents": "Acute renal failure subsequent to the administration of rifampicin. A follow-up study of cases reported earlier. A clinical presentation is made of a 2-3 year follow-up of six cases of acute renal failure that have been reported earlier. The patients had developed transient renal failure after the intermittent administration of rifampicin. The stage of olig-anuria lasted for 1-3 weeks, and five of the patients were treated by hemodialysis. Two of the patients died due to unrelated causes during the follow-up period. The four patients re-examined were clinically cured. Pathologic findings by light microscopy and immunofluorescence at biopsy were scarce. Nothing abnormal was seen by electron microscopy in two of the cases studied. Renal function was normal. In three cases the excretion at 131I-hippuran renography was slightly slowed. Although in the acute stage the renal lesions histologically appeared toxic, evidence suggestive of an immunological mechanism cannot be excluded.", "PMID": 982002}
{"id": "pubmed23n0032_12855", "title": "[Prognostic factors concerning the survival time of Hodgkin's disease stage III and IV, treated with cytostatic agents].", "content": "On the basis of a retrospective study of 79 well-documented cases of Hodgkin's disease it has been possible to determine the prognostic significance of several factors in regard to 3-year survival. All patients evaluated were in stage III or IV and receiving polychemotherapy. According to the survival curves, the following factors adversely affected the prognosis to a significant extent: clinical stage IV, the presence of systemic symptoms, male sex, and failure to receive maintenance therapy. Also, patients treated by polychemotherapy only on relapse following other prior treatment, had a somewhat worse prognosis, though in out patient material this was not statistically significant. Other criteria significantly affecting the probability of 3-year survival in a negative sense were: decreased activity index, anemia, lymphopenia, elevated alkaline phosphatase values, low serum albumin and high serum globulin values. Elevated sedimentation rate, leukocytosis and leukopenia had no statistically significant influence upon survival.", "contents": "[Prognostic factors concerning the survival time of Hodgkin's disease stage III and IV, treated with cytostatic agents]. On the basis of a retrospective study of 79 well-documented cases of Hodgkin's disease it has been possible to determine the prognostic significance of several factors in regard to 3-year survival. All patients evaluated were in stage III or IV and receiving polychemotherapy. According to the survival curves, the following factors adversely affected the prognosis to a significant extent: clinical stage IV, the presence of systemic symptoms, male sex, and failure to receive maintenance therapy. Also, patients treated by polychemotherapy only on relapse following other prior treatment, had a somewhat worse prognosis, though in out patient material this was not statistically significant. Other criteria significantly affecting the probability of 3-year survival in a negative sense were: decreased activity index, anemia, lymphopenia, elevated alkaline phosphatase values, low serum albumin and high serum globulin values. Elevated sedimentation rate, leukocytosis and leukopenia had no statistically significant influence upon survival.", "PMID": 982004}
{"id": "pubmed23n0032_12856", "title": "[HL-A system and diabetes mellitus].", "content": "In 19 families of juvenile diabetes patients intravenous glucose tolerance was tested and HLA antigens were determined. A total of 68 first degree blood relations (siblings, parents, children) was studied. Taking the age dependent variabilities of the glucose assimilation coefficient (k-value) into consideration, glucose intolerance was found in 35.5% of the blood relations. Particularly in blood relations (above all in siblings) aged under 35 and with glucose intolerance, a trend to increased frequencies of those HLA antigens (B8, BW15, CW3) associated with juvenile diabetes was found, but it is not yet clear whether this association will be of practical significance.", "contents": "[HL-A system and diabetes mellitus]. In 19 families of juvenile diabetes patients intravenous glucose tolerance was tested and HLA antigens were determined. A total of 68 first degree blood relations (siblings, parents, children) was studied. Taking the age dependent variabilities of the glucose assimilation coefficient (k-value) into consideration, glucose intolerance was found in 35.5% of the blood relations. Particularly in blood relations (above all in siblings) aged under 35 and with glucose intolerance, a trend to increased frequencies of those HLA antigens (B8, BW15, CW3) associated with juvenile diabetes was found, but it is not yet clear whether this association will be of practical significance.", "PMID": 982005}
{"id": "pubmed23n0032_12857", "title": "[Cytodiagnosis of the uterine portio vaginalis according to WHO nomenclature. I. Correlative revision of cytological and histological diagnoses as quality control of selected materials. Frequency and possible reasons for the lack of correlation of cytological and histological diagnoses].", "content": "Smears from the uterine cervix have been evaluated for neoplastic changes on the basis of descriptive terms. The material was obtained by a single gynecologist using a standardized sampling technique and the results were compared with the respective histological diagnoses. Material which was found to be unsuitable for technical reasons was excluded from this study. A 98.7% agreement between cytological and histological findings was noted. Discrepancies between cytological and histological findings were essentially due to differing dysplastic changes in the deeper layers of the surface epithelium and the cervical glands, which were not sampled by the smears on the surface layer. In a few cases the discrepancies could not be explained but they disappeared after several years of observation. The practitioner should be acquainted with the nomenclature used by cytologists. In his turn, the cytologist should be familiar with the pathological anatomy of the organ in question.", "contents": "[Cytodiagnosis of the uterine portio vaginalis according to WHO nomenclature. I. Correlative revision of cytological and histological diagnoses as quality control of selected materials. Frequency and possible reasons for the lack of correlation of cytological and histological diagnoses]. Smears from the uterine cervix have been evaluated for neoplastic changes on the basis of descriptive terms. The material was obtained by a single gynecologist using a standardized sampling technique and the results were compared with the respective histological diagnoses. Material which was found to be unsuitable for technical reasons was excluded from this study. A 98.7% agreement between cytological and histological findings was noted. Discrepancies between cytological and histological findings were essentially due to differing dysplastic changes in the deeper layers of the surface epithelium and the cervical glands, which were not sampled by the smears on the surface layer. In a few cases the discrepancies could not be explained but they disappeared after several years of observation. The practitioner should be acquainted with the nomenclature used by cytologists. In his turn, the cytologist should be familiar with the pathological anatomy of the organ in question.", "PMID": 982006}
{"id": "pubmed23n0032_12858", "title": "[Cytodiagnosis of the uterine portio vaginalis according to WHO nomenclature. II. Clinical implications of descriptive cytological evaluations].", "content": "The evaluation of smears of the uterine cervix on the basis of descriptive terms requires the practitioner to be acquainted with the nomenclature used by cytologists. A theoretical scheme is provided which correlates the diagnostic features encountered with their respective clinical implications.", "contents": "[Cytodiagnosis of the uterine portio vaginalis according to WHO nomenclature. II. Clinical implications of descriptive cytological evaluations]. The evaluation of smears of the uterine cervix on the basis of descriptive terms requires the practitioner to be acquainted with the nomenclature used by cytologists. A theoretical scheme is provided which correlates the diagnostic features encountered with their respective clinical implications.", "PMID": 982007}
{"id": "pubmed23n0032_12859", "title": "[Malignant peritoneal mesothelioma].", "content": "A case of diffused malignant mesothelioma of the peritoneum in a 64-year-old man is presented. This mesothelioma, of the papillary type, was complicated, after a year without significant clinical symptoms, by the development of ascites and obstructive upper bowel ileus. Physiological-anatomical and clinical aspects, as well as special radiologic findings and therapeutic regimens, are discussed in the light of the literature. It is recommended that all patients with malignant mesothelioma of the peritoneum be treated by total abdominal irradiation and intreperitoneal instillation of colloid P32.", "contents": "[Malignant peritoneal mesothelioma]. A case of diffused malignant mesothelioma of the peritoneum in a 64-year-old man is presented. This mesothelioma, of the papillary type, was complicated, after a year without significant clinical symptoms, by the development of ascites and obstructive upper bowel ileus. Physiological-anatomical and clinical aspects, as well as special radiologic findings and therapeutic regimens, are discussed in the light of the literature. It is recommended that all patients with malignant mesothelioma of the peritoneum be treated by total abdominal irradiation and intreperitoneal instillation of colloid P32.", "PMID": 982008}
{"id": "pubmed23n0032_12860", "title": "[Ayurveda].", "content": "The ancient medicine of India (ayurveda) is a broadly conceptualized anthropology embracing the whole human being without the Western body/soul dualism. A symptomatology based on careful observation leads, in conjunction with etiology, pathogenesis and pathology, with constitutional and personality features, to a differentiated nosology. This broad concept is matched by multidimensional therapy embracing pharmacologic, surgical, psychotherapeutic and \"paramedical\" measures. Ayurveda (\"life knowledge\") is a combination of religious, philosophical, anthropological, medical and psychological concepts. It is impressive how accurate the knowledge and description of psychiatric diseases already was 2,500 years ago, and how little these phenomena have changed with time. The therapeutic armamentarium (paramedical, psychotherapeutic and pharmacologic) of ayurveda psychiatry has not yet been sufficiently studied by scientific methods.", "contents": "[Ayurveda]. The ancient medicine of India (ayurveda) is a broadly conceptualized anthropology embracing the whole human being without the Western body/soul dualism. A symptomatology based on careful observation leads, in conjunction with etiology, pathogenesis and pathology, with constitutional and personality features, to a differentiated nosology. This broad concept is matched by multidimensional therapy embracing pharmacologic, surgical, psychotherapeutic and \"paramedical\" measures. Ayurveda (\"life knowledge\") is a combination of religious, philosophical, anthropological, medical and psychological concepts. It is impressive how accurate the knowledge and description of psychiatric diseases already was 2,500 years ago, and how little these phenomena have changed with time. The therapeutic armamentarium (paramedical, psychotherapeutic and pharmacologic) of ayurveda psychiatry has not yet been sufficiently studied by scientific methods.", "PMID": 982009}
{"id": "pubmed23n0032_12861", "title": "[Hormonal treatment of hirsutism].", "content": "In 44 hirsute women treated with antiandrogens, sequential administration of cyproterone acetate and ethinylestradiol produced a success rate of 87%. The mean duration of treatment was 9 months, and side effects were rare. Antiandrogens are therefore superior to conventional cosmetic methods, though prior hormonal analysis is indispensable.", "contents": "[Hormonal treatment of hirsutism]. In 44 hirsute women treated with antiandrogens, sequential administration of cyproterone acetate and ethinylestradiol produced a success rate of 87%. The mean duration of treatment was 9 months, and side effects were rare. Antiandrogens are therefore superior to conventional cosmetic methods, though prior hormonal analysis is indispensable.", "PMID": 982010}
{"id": "pubmed23n0032_12862", "title": "[Mesosystolic click syndrome and arrhythmia].", "content": "On 24-hour ambulatory ECG, 17 patients with mitral valve prolapse displayed more frequent and serious arrhythmias than a control group of 17. Neither resting ECG, treadmill ECG, nor other external recordings were capable of predicting which of the patients were prone to development of arrhythmias.", "contents": "[Mesosystolic click syndrome and arrhythmia]. On 24-hour ambulatory ECG, 17 patients with mitral valve prolapse displayed more frequent and serious arrhythmias than a control group of 17. Neither resting ECG, treadmill ECG, nor other external recordings were capable of predicting which of the patients were prone to development of arrhythmias.", "PMID": 982011}
{"id": "pubmed23n0032_12863", "title": "[The benign symmetrical lipomatosis (adenolipomatosis Launois-Bensaude; Madelung's fat neck)].", "content": "A case of benign symmetric lipomatosis is reported in conjunction with a review of the literature. The association with metabolic disorders such as gout and hyperlipemia, as reported by others, is fortuitous and not statistically significant. The etiology of this rare type of lipomatosis is unknown; its treatment is surgical and should be confined to cases with symptoms of compression or extremely disturbing appearance.", "contents": "[The benign symmetrical lipomatosis (adenolipomatosis Launois-Bensaude; Madelung's fat neck)]. A case of benign symmetric lipomatosis is reported in conjunction with a review of the literature. The association with metabolic disorders such as gout and hyperlipemia, as reported by others, is fortuitous and not statistically significant. The etiology of this rare type of lipomatosis is unknown; its treatment is surgical and should be confined to cases with symptoms of compression or extremely disturbing appearance.", "PMID": 982012}
{"id": "pubmed23n0032_12864", "title": "[Isolated decalcifying algodystrophy of the hip].", "content": "Transitory demineralization of the hip, a special form of reflex sympathetic dystrophy, is a rare condition. It should however be distinguished from more severe conditions such as tuberculosis or neoplastic bone disease. The typical symptoms are illustrated from 3 personally observed cases. The patients, usually men between the ages of 30 and 50 years or women in the last months of pregnancy, suffer severe mechanical pain in the hip, with limping and weakness. They usually need crutches for walking. Clinical examination of the hip reveals only slight restriction of passive movements. The erythrocyte sedimentation rate may be slightly elevated. Bone scan discloses hypercaptation, while other laboratory tests are negative. A few weeks after onset of symptoms, pelvic X-rays show extreme demineralization of the femoral head. On the other hand, the joint space and bony contours remain intact. The prognosis is excellent and the symptoms disappear after a few months or after delivery.", "contents": "[Isolated decalcifying algodystrophy of the hip]. Transitory demineralization of the hip, a special form of reflex sympathetic dystrophy, is a rare condition. It should however be distinguished from more severe conditions such as tuberculosis or neoplastic bone disease. The typical symptoms are illustrated from 3 personally observed cases. The patients, usually men between the ages of 30 and 50 years or women in the last months of pregnancy, suffer severe mechanical pain in the hip, with limping and weakness. They usually need crutches for walking. Clinical examination of the hip reveals only slight restriction of passive movements. The erythrocyte sedimentation rate may be slightly elevated. Bone scan discloses hypercaptation, while other laboratory tests are negative. A few weeks after onset of symptoms, pelvic X-rays show extreme demineralization of the femoral head. On the other hand, the joint space and bony contours remain intact. The prognosis is excellent and the symptoms disappear after a few months or after delivery.", "PMID": 982014}
{"id": "pubmed23n0032_12865", "title": "[Pyrithyldione excretion in the urine. Methods and results from various collectives].", "content": "A simple thinlayer chromatographic method for the detection of pyrithyldion in 5 ml of urine per analysis is described. Sensitivity (0.01 mug of pyrithyldion) and specificity (reaction still positive 5-6 days after injection of 50 mg of pyrithyldion) of the method are considered acceptable. The duration of elimination for pyrithyldion is relatively long (5-6 days). Epidemiological investigations were conducted in two different patient groups to analyze spontaneously voided urine samples. The incidence of positive urine samples in 204 ambulatory internal medicine patients was only 0.5% for pyrithyldion and N-acetyl-p-amino-phenol (the main metabolite of phenacetin). In contrast, the incidence in a specially selected group of 600 working women with regular intake of analgesics was much higher (between 17.7% and 28%) in the period 1969 to 1972.", "contents": "[Pyrithyldione excretion in the urine. Methods and results from various collectives]. A simple thinlayer chromatographic method for the detection of pyrithyldion in 5 ml of urine per analysis is described. Sensitivity (0.01 mug of pyrithyldion) and specificity (reaction still positive 5-6 days after injection of 50 mg of pyrithyldion) of the method are considered acceptable. The duration of elimination for pyrithyldion is relatively long (5-6 days). Epidemiological investigations were conducted in two different patient groups to analyze spontaneously voided urine samples. The incidence of positive urine samples in 204 ambulatory internal medicine patients was only 0.5% for pyrithyldion and N-acetyl-p-amino-phenol (the main metabolite of phenacetin). In contrast, the incidence in a specially selected group of 600 working women with regular intake of analgesics was much higher (between 17.7% and 28%) in the period 1969 to 1972.", "PMID": 982015}
{"id": "pubmed23n0032_12866", "title": "[Long-term results of fasting. Results from 132 consecutive patients after 1-6 1/2 years].", "content": "132 consecutive patients were reexamined 1-6.5 years (m = 2.8) after total fasting in hospital. 37 patients had continued to lose weight, 20 patients had maintained their fasting result (+/-2 kg) and 7 patients had slightly regained (less than 1/3 of their weight loss during fast). These 3 groups, totalling 64 patients (48%), were considered successful. Their mean overweight was diminished from 57 to 29%, corresponding to a 60% reduction of mortality in certain age groups. On the other hand, 63 patients (48%) had regained more than 1/3 of their original weight loss. Five patients (4%) were lost to follow-up. Selection of patients and long-term follow-up appeared to have a decisive bearing on long-term results of fasting, whereas factors such as age, sex, degree of overweight, onset of overweight in childhood and sports were without significant effect. From the long-term results presented, it is concluded that under certain conditions fasting in hospital is warranted. Behaviour therapy is a possible alternative in the treatment of obesity.", "contents": "[Long-term results of fasting. Results from 132 consecutive patients after 1-6 1/2 years]. 132 consecutive patients were reexamined 1-6.5 years (m = 2.8) after total fasting in hospital. 37 patients had continued to lose weight, 20 patients had maintained their fasting result (+/-2 kg) and 7 patients had slightly regained (less than 1/3 of their weight loss during fast). These 3 groups, totalling 64 patients (48%), were considered successful. Their mean overweight was diminished from 57 to 29%, corresponding to a 60% reduction of mortality in certain age groups. On the other hand, 63 patients (48%) had regained more than 1/3 of their original weight loss. Five patients (4%) were lost to follow-up. Selection of patients and long-term follow-up appeared to have a decisive bearing on long-term results of fasting, whereas factors such as age, sex, degree of overweight, onset of overweight in childhood and sports were without significant effect. From the long-term results presented, it is concluded that under certain conditions fasting in hospital is warranted. Behaviour therapy is a possible alternative in the treatment of obesity.", "PMID": 982016}
{"id": "pubmed23n0032_12867", "title": "[Splenic angioma with thrombocytopenia].", "content": "A case of splenectomy with thrombocytopenia but without hypersplenism is described. The etiology, proven by splenectomy, was a splenic angioma. The isotopic examinations necessary to localize the site of a vascular tumor and the relations between thrombocytopenia and hemangioma are discussed.", "contents": "[Splenic angioma with thrombocytopenia]. A case of splenectomy with thrombocytopenia but without hypersplenism is described. The etiology, proven by splenectomy, was a splenic angioma. The isotopic examinations necessary to localize the site of a vascular tumor and the relations between thrombocytopenia and hemangioma are discussed.", "PMID": 982017}
{"id": "pubmed23n0032_12868", "title": "Reinnervation of dentate gyrus by homologous afferents following entorhinal cortical lesions in adult rats.", "content": "Granule cells of the rat dentate gyrus which are denervated by unilateral destruction of the entorhinal cortex are reinnervated in part by proliferation of surviving pathways from the contralateral entorhinal cortex. The cells of origin of these lesion-induced projections were identified by retrograde labeling with horseradish peroxidase and were the same cell type which normally project to the ipsilateral dentate gyrus", "contents": "Reinnervation of dentate gyrus by homologous afferents following entorhinal cortical lesions in adult rats. Granule cells of the rat dentate gyrus which are denervated by unilateral destruction of the entorhinal cortex are reinnervated in part by proliferation of surviving pathways from the contralateral entorhinal cortex. The cells of origin of these lesion-induced projections were identified by retrograde labeling with horseradish peroxidase and were the same cell type which normally project to the ipsilateral dentate gyrus", "PMID": 982024}
{"id": "pubmed23n0032_12869", "title": "Activation of histone gene transcription by nonhistone chromosomal phosphoproteins.", "content": "Hybridization analysis of RNA transcripts from HeLa S3 cell chromatin to histone complementary DNA indicates that a chromosomal phosphoprotein fraction activates transcription of histone messenger RNA sequences in vitro with chromatin from a phase in the cell cycle when histone genes are normally silent.", "contents": "Activation of histone gene transcription by nonhistone chromosomal phosphoproteins. Hybridization analysis of RNA transcripts from HeLa S3 cell chromatin to histone complementary DNA indicates that a chromosomal phosphoprotein fraction activates transcription of histone messenger RNA sequences in vitro with chromatin from a phase in the cell cycle when histone genes are normally silent.", "PMID": 982025}
{"id": "pubmed23n0032_12870", "title": "5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells.", "content": "The nucleoside analog 5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole (DRB) at 75 to 150 micromolar concentrations inhibits the synthesis of nuclear heterogeneous RNA (hnRNA) in HeLa cells by 60 to 70 percent. The sedimentation profile of hnRNA labeled with (3H)uridine for 45 seconds after brief treatment (45, 90, or 180 seconds) with DRB showed a progressive decrease in the labeling of shorter hnRNA molecules relative to longer molecules. Prior exposure of the cells to actinomycin D, an inhibitor of RNA chain elongation, did not alter the sedimentation profile of hnRNA. These results suggest that DRB preferentially inhibits the initiation of hnRNA chains so that after exposure to DRB for a brief period the longer nascent chains still remain to be finished and thus incorporate a greater share of the pulse label. By progressively increasing the time of exposure to DRB, and measuring the rate of increase in the average size of the labeled, nascent RNA, it was estimated that the chains were growing at rates between 50 and 100 nucleotides per second.", "contents": "5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells. The nucleoside analog 5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole (DRB) at 75 to 150 micromolar concentrations inhibits the synthesis of nuclear heterogeneous RNA (hnRNA) in HeLa cells by 60 to 70 percent. The sedimentation profile of hnRNA labeled with (3H)uridine for 45 seconds after brief treatment (45, 90, or 180 seconds) with DRB showed a progressive decrease in the labeling of shorter hnRNA molecules relative to longer molecules. Prior exposure of the cells to actinomycin D, an inhibitor of RNA chain elongation, did not alter the sedimentation profile of hnRNA. These results suggest that DRB preferentially inhibits the initiation of hnRNA chains so that after exposure to DRB for a brief period the longer nascent chains still remain to be finished and thus incorporate a greater share of the pulse label. By progressively increasing the time of exposure to DRB, and measuring the rate of increase in the average size of the labeled, nascent RNA, it was estimated that the chains were growing at rates between 50 and 100 nucleotides per second.", "PMID": 982026}
{"id": "pubmed23n0032_12871", "title": "A model of nitrogenase active-centre and mechanism of nitrogenase catalysis.", "content": "Based upon the known reactions of nitrogenase and the principles of coordination catalysis, a model of nitrogenase active-centre is proposed. An octa-atomic cluster, Fe2S2-Mo2O2, of pseudo-cubane-type structure is supposed to form a coupled twin of trinuclear (2Mo-1Fe) active-centre, which also catalyzes the reduction of H+ to H2. With this model, mechanisms of all the known nitrogenase-catalyzed reactions are explained, together with the non-inhibition of the hydrogen-evolution reaction by CO, and the mixed character of inhibition of other nitrogenase-catalyzed reactions. Electron transport by 2-stepped ATP-driving with some electron back-flow is shown to give a reasonable explanation for the observed ATP/2e- ratio and for the reductant-independent ATP hydrolysis catalyzed by nitrogenase. The close analogy between electron transport by 2-stepped ATP-driving in nitrogenase catalysis and electron transport by 2-stepped photo-driving in photosynthesis by green plants in illustrated.", "contents": "A model of nitrogenase active-centre and mechanism of nitrogenase catalysis. Based upon the known reactions of nitrogenase and the principles of coordination catalysis, a model of nitrogenase active-centre is proposed. An octa-atomic cluster, Fe2S2-Mo2O2, of pseudo-cubane-type structure is supposed to form a coupled twin of trinuclear (2Mo-1Fe) active-centre, which also catalyzes the reduction of H+ to H2. With this model, mechanisms of all the known nitrogenase-catalyzed reactions are explained, together with the non-inhibition of the hydrogen-evolution reaction by CO, and the mixed character of inhibition of other nitrogenase-catalyzed reactions. Electron transport by 2-stepped ATP-driving with some electron back-flow is shown to give a reasonable explanation for the observed ATP/2e- ratio and for the reductant-independent ATP hydrolysis catalyzed by nitrogenase. The close analogy between electron transport by 2-stepped ATP-driving in nitrogenase catalysis and electron transport by 2-stepped photo-driving in photosynthesis by green plants in illustrated.", "PMID": 982022}
{"id": "pubmed23n0032_12872", "title": "Jackrabbit ears: surface temperatures and vascular responses.", "content": "Blood flow to the ear pinnae is curtailed at ambient temperatures of between 1.4 degrees and 24 degrees C, which minimizes heat loss across the pinnae and allows the surfaces of erect pinnae to approach ambient temperature. The pinnae are warmed by steady or pulsatile vasodilation in some animals when the ambient temperature is between 1 degree and 9 degrees C below body temperature, a response favoring heat loss. When ambient temperature exceeds body temperature by 4 degrees to 5 degrees C, the pinnae are circulated with blood cooler than ambient temperature; this response favors heat influx.", "contents": "Jackrabbit ears: surface temperatures and vascular responses. Blood flow to the ear pinnae is curtailed at ambient temperatures of between 1.4 degrees and 24 degrees C, which minimizes heat loss across the pinnae and allows the surfaces of erect pinnae to approach ambient temperature. The pinnae are warmed by steady or pulsatile vasodilation in some animals when the ambient temperature is between 1 degree and 9 degrees C below body temperature, a response favoring heat loss. When ambient temperature exceeds body temperature by 4 degrees to 5 degrees C, the pinnae are circulated with blood cooler than ambient temperature; this response favors heat influx.", "PMID": 982027}
{"id": "pubmed23n0032_12873", "title": "Hydra hymanae: regulation of the life cycle by time and temperature.", "content": "Hydra hymanae, a hermaphroditic freshwater coelenterate, reproduces asexually at 24 degrees C and sexually at 15 degrees C. The appearance of gonads begins 12 days after transfer from 24 degrees to 15 degrees C and is complete 35 days after the temperature transition. Testes appear before eggs. Fifty percent of the mature embryos maintained at 15 degrees C hatch by day 61, but they have a low level of survival. Fifty percent of the mature embryos pretreated for from 5 to 25 days at 4 degrees C hatch by about day 45, and these have a high level of survival. Embryos maintained at 4 degrees C for longer periods (55 to 85 days) accumulate in a prehatching state and hatch with a high degree of synchrony approximately 7.5 days after return to 15 degrees C. Populations derived from newly hatched polyps are refractory to sex induction for approximately 120 days. The system is well adapted to ensure a regular alternation of reproductive modes in the natural environment.", "contents": "Hydra hymanae: regulation of the life cycle by time and temperature. Hydra hymanae, a hermaphroditic freshwater coelenterate, reproduces asexually at 24 degrees C and sexually at 15 degrees C. The appearance of gonads begins 12 days after transfer from 24 degrees to 15 degrees C and is complete 35 days after the temperature transition. Testes appear before eggs. Fifty percent of the mature embryos maintained at 15 degrees C hatch by day 61, but they have a low level of survival. Fifty percent of the mature embryos pretreated for from 5 to 25 days at 4 degrees C hatch by about day 45, and these have a high level of survival. Embryos maintained at 4 degrees C for longer periods (55 to 85 days) accumulate in a prehatching state and hatch with a high degree of synchrony approximately 7.5 days after return to 15 degrees C. Populations derived from newly hatched polyps are refractory to sex induction for approximately 120 days. The system is well adapted to ensure a regular alternation of reproductive modes in the natural environment.", "PMID": 982029}
{"id": "pubmed23n0032_12874", "title": "Amianthoid change: orientation of normal collagen fibrils during aging.", "content": "High-angle x-ray diffraction provides direct evidence that amianthoid change, occurring during aging of costal cartilage, corresponds to a transformation from an isotropic to a marked anisotropic distribution of collagen fibrils. Low-angle x-ray diffraction and electron microscopy show that the fibrils have the customary 67-nanometer axial periodicity. Electron microscopy shows that wide amianthoid collagen fibrils consist of smaller parallel fibrils fused together. Similarities between amianthoid change and tendon morphogenesis are briefly discussed. Amianthoid change is remarkable in that aging is accompanied by increased order.", "contents": "Amianthoid change: orientation of normal collagen fibrils during aging. High-angle x-ray diffraction provides direct evidence that amianthoid change, occurring during aging of costal cartilage, corresponds to a transformation from an isotropic to a marked anisotropic distribution of collagen fibrils. Low-angle x-ray diffraction and electron microscopy show that the fibrils have the customary 67-nanometer axial periodicity. Electron microscopy shows that wide amianthoid collagen fibrils consist of smaller parallel fibrils fused together. Similarities between amianthoid change and tendon morphogenesis are briefly discussed. Amianthoid change is remarkable in that aging is accompanied by increased order.", "PMID": 982030}
{"id": "pubmed23n0032_12875", "title": "Extended helical conformation newly observed in protein folding.", "content": "A new secondary structure, which shows regularity within the experimental error, is noticed in alpha-chymotrypsin, and considering its extended nature, the name epsilon-helix has been suggested for the same. The average observed values of phi and psi for this conformation are -93 degrees and +146 degrees, respectively. The helical parameters turn out to be n = 2.7 and h = 3.3 angstroms.", "contents": "Extended helical conformation newly observed in protein folding. A new secondary structure, which shows regularity within the experimental error, is noticed in alpha-chymotrypsin, and considering its extended nature, the name epsilon-helix has been suggested for the same. The average observed values of phi and psi for this conformation are -93 degrees and +146 degrees, respectively. The helical parameters turn out to be n = 2.7 and h = 3.3 angstroms.", "PMID": 982035}
{"id": "pubmed23n0032_12876", "title": "Regulation of acetylcholine synthesis: does cytoplasmic acetylcholine control high affinity choline uptake?", "content": "When brain synaptosomes are obtained from animals that have been injected intravenously with [2H4]choline 1 minute before being killed, their high affinity [3H] choline uptake is correlated inversely with their acetylcholine content and directly with the rate at which they synthesize [2H4]acetylcholine. The control of such choline uptake by the cytoplasmic acetylcholine concentration is proposed as a mechanism regulating acetylcholine synthesis in cholinergic nerve terminals.", "contents": "Regulation of acetylcholine synthesis: does cytoplasmic acetylcholine control high affinity choline uptake? When brain synaptosomes are obtained from animals that have been injected intravenously with [2H4]choline 1 minute before being killed, their high affinity [3H] choline uptake is correlated inversely with their acetylcholine content and directly with the rate at which they synthesize [2H4]acetylcholine. The control of such choline uptake by the cytoplasmic acetylcholine concentration is proposed as a mechanism regulating acetylcholine synthesis in cholinergic nerve terminals.", "PMID": 982031}
{"id": "pubmed23n0032_12877", "title": "Tail-pinch stimulation: sufficient motivation for learning.", "content": "A paper clip applied to the tails of rats induced gnawing and eating, which decreased in latency and increased in duration with experience. With sustained pressure to the tail, rats learned a new habit in order to gain access to wood chips on which to gnaw. That these are also properties of behavior elicited by electrical brain stimulation suggests that both manipulations may act through the same mechanism. These results support the hypothesis that a nonspecific arousing stimulus can be a sufficient condition for establishing learned habits.", "contents": "Tail-pinch stimulation: sufficient motivation for learning. A paper clip applied to the tails of rats induced gnawing and eating, which decreased in latency and increased in duration with experience. With sustained pressure to the tail, rats learned a new habit in order to gain access to wood chips on which to gnaw. That these are also properties of behavior elicited by electrical brain stimulation suggests that both manipulations may act through the same mechanism. These results support the hypothesis that a nonspecific arousing stimulus can be a sufficient condition for establishing learned habits.", "PMID": 982032}
{"id": "pubmed23n0032_12878", "title": "Sodium liquid ion exchanger microelectrode used to measure large extracellular sodium transients.", "content": "A new liquid membrane microelectrode has been developed that is easily fabricated and can measure fast sodium transients in the presence of potassium interference. It responds to a sudden change in sodium activity within 1 second. The electrode has been used to provide the first direct evidence of large sodium transients in the extracellular space of the brain of the catfish.", "contents": "Sodium liquid ion exchanger microelectrode used to measure large extracellular sodium transients. A new liquid membrane microelectrode has been developed that is easily fabricated and can measure fast sodium transients in the presence of potassium interference. It responds to a sudden change in sodium activity within 1 second. The electrode has been used to provide the first direct evidence of large sodium transients in the extracellular space of the brain of the catfish.", "PMID": 982036}
{"id": "pubmed23n0032_12879", "title": "Stereochemical requirements for intercalation of platinum complexes into double-stranded DNA's.", "content": "The complexes 1,10-phenanthrolineethylenediamineplatinum(II) and 2,2'-bipyridineethylenediamineplatinum(II) have a planar, aromatic ligand system that facilitates intercalation, as shown by their ability to unwind closed circular duplex DNA. Nonbonded steric interactions can rotate the pryidine ligands out of the coordination plane in bis(pyridine)ethylenediamineplatinum(II), thus preventing intercalation. Fiber x-ray diffraction patterns of the two metallointeracalators indicate that the binding is governed by the neighbor exclusion principle.", "contents": "Stereochemical requirements for intercalation of platinum complexes into double-stranded DNA's. The complexes 1,10-phenanthrolineethylenediamineplatinum(II) and 2,2'-bipyridineethylenediamineplatinum(II) have a planar, aromatic ligand system that facilitates intercalation, as shown by their ability to unwind closed circular duplex DNA. Nonbonded steric interactions can rotate the pryidine ligands out of the coordination plane in bis(pyridine)ethylenediamineplatinum(II), thus preventing intercalation. Fiber x-ray diffraction patterns of the two metallointeracalators indicate that the binding is governed by the neighbor exclusion principle.", "PMID": 982037}
{"id": "pubmed23n0032_12880", "title": "Sex-specific cannibalism in the rotifer Asplanchna sieboldi.", "content": "In one clone, large-morphotype females rarely exhibit tactile feeding responses to their ingestible, male clonemates but readily attack small-morphotype female clonemates and males of another, taxonomically distinct, clone. In the latter clone, cannibal females lack such selectivity, but their males are well protected from capture by very large, lateral body-wall outgrowths.", "contents": "Sex-specific cannibalism in the rotifer Asplanchna sieboldi. In one clone, large-morphotype females rarely exhibit tactile feeding responses to their ingestible, male clonemates but readily attack small-morphotype female clonemates and males of another, taxonomically distinct, clone. In the latter clone, cannibal females lack such selectivity, but their males are well protected from capture by very large, lateral body-wall outgrowths.", "PMID": 982038}
{"id": "pubmed23n0032_12881", "title": "Sleep in mammals: ecological and constitutional correlates.", "content": "The interrelationships between sleep, ecological, and constitutional variables were assessed statistically for 39 mammalian species. Slow-wave sleep is negatively associated with a factor related to body size, which suggests that large amounts of this sleep phase are disadvantageous in large species. Paradoxical sleep is associated with a factor related to predatory danger, which suggests that large amounts of this sleep phase are disadvantageous in prey species.", "contents": "Sleep in mammals: ecological and constitutional correlates. The interrelationships between sleep, ecological, and constitutional variables were assessed statistically for 39 mammalian species. Slow-wave sleep is negatively associated with a factor related to body size, which suggests that large amounts of this sleep phase are disadvantageous in large species. Paradoxical sleep is associated with a factor related to predatory danger, which suggests that large amounts of this sleep phase are disadvantageous in prey species.", "PMID": 982039}
{"id": "pubmed23n0032_12882", "title": "Filaroides hirthi: experimental transmission among beagle dogs through ingestion of first-stage larvae.", "content": "Four beagle dogs were found to be infected with sexually mature Filaroides hirthi lungworms from 1 to 3 months after they ingested material containing first-stage larvae opens the theoretical possibility of autoinfection of which few nematode parasites are capable.", "contents": "Filaroides hirthi: experimental transmission among beagle dogs through ingestion of first-stage larvae. Four beagle dogs were found to be infected with sexually mature Filaroides hirthi lungworms from 1 to 3 months after they ingested material containing first-stage larvae opens the theoretical possibility of autoinfection of which few nematode parasites are capable.", "PMID": 982040}
{"id": "pubmed23n0032_12883", "title": "Antiserum to brain gangliosides produces recurrent epileptiform activity.", "content": "A single injection of 10 microliters of antiserum to total brain ganglioside onto (and into) the sensorimotor cortex of the rat resulted in recurrent spiking in the cortical electroencephalogram, lasting from 7 to 17 days. Absorption of antibody with pure monosialoganglioside (GM1) completely abosished the effect. Spiking was reactivated after 4 weeks by intramuscular injection of pentylenetetrazole.", "contents": "Antiserum to brain gangliosides produces recurrent epileptiform activity. A single injection of 10 microliters of antiserum to total brain ganglioside onto (and into) the sensorimotor cortex of the rat resulted in recurrent spiking in the cortical electroencephalogram, lasting from 7 to 17 days. Absorption of antibody with pure monosialoganglioside (GM1) completely abosished the effect. Spiking was reactivated after 4 weeks by intramuscular injection of pentylenetetrazole.", "PMID": 982041}
{"id": "pubmed23n0032_12884", "title": "A mallophaga, Trinoton anserinum, as a cyclodevelopmental vector for a heartworm parasite of waterfowl.", "content": "The biting louse Trinoton anserinum serves as the intermediate host in the life cycle of the filarial heartworm, Sarconema eurycerca. Microfilariae, second-, and third-stage larvae were dissected from 39 of 89 lice infesting whistling swans, Cygnus columbianus columbianus, in North America and mute swans, Cygnus olor, in the Black Sea, U.S.S.R. Infective third-stage larvae obtained from lice collected from heartworm-parasitized whistling swans were injected subcutaneously into each of two hand-reared, nonparasitized mute swan cygnets. Both of these birds developed heartworm infections, one becoming microfilaremic at 14 weeks. The results of this study provide conclusive evidence that a mallophagan serves as a natural cyclodevelopmental vector of a filarial parasite.", "contents": "A mallophaga, Trinoton anserinum, as a cyclodevelopmental vector for a heartworm parasite of waterfowl. The biting louse Trinoton anserinum serves as the intermediate host in the life cycle of the filarial heartworm, Sarconema eurycerca. Microfilariae, second-, and third-stage larvae were dissected from 39 of 89 lice infesting whistling swans, Cygnus columbianus columbianus, in North America and mute swans, Cygnus olor, in the Black Sea, U.S.S.R. Infective third-stage larvae obtained from lice collected from heartworm-parasitized whistling swans were injected subcutaneously into each of two hand-reared, nonparasitized mute swan cygnets. Both of these birds developed heartworm infections, one becoming microfilaremic at 14 weeks. The results of this study provide conclusive evidence that a mallophagan serves as a natural cyclodevelopmental vector of a filarial parasite.", "PMID": 982042}
{"id": "pubmed23n0032_12885", "title": "An animal behavior model for studying the actions of LSD and related hallucinogens.", "content": "Cats injected with LSD (d-lysergic acid diethylamide) exhibit a group of behaviors that appear to be specific to hallucinogenic drugs. Two of these behaviors, limb flick and abortive grooming, have an extremely low frequency of occurrence in normal cats, but often dominate the behavior of LSD-treated cats. The frequency of occurrence of this group of behaviors is related to the dose of LSD. The behavioral changes are long-lasting following a single injection of LSD, and exhibit tolerance following the repeated administration of LSD. They are not elicited by a variety of control drugs, but are elicited by other indole nucleus hallucinogens. Because the behavioral effects are specific, reliable, easy to score, and quantifiable, they represent an animal model that can be used in studies of the effects of LSD and related hallucinogens.", "contents": "An animal behavior model for studying the actions of LSD and related hallucinogens. Cats injected with LSD (d-lysergic acid diethylamide) exhibit a group of behaviors that appear to be specific to hallucinogenic drugs. Two of these behaviors, limb flick and abortive grooming, have an extremely low frequency of occurrence in normal cats, but often dominate the behavior of LSD-treated cats. The frequency of occurrence of this group of behaviors is related to the dose of LSD. The behavioral changes are long-lasting following a single injection of LSD, and exhibit tolerance following the repeated administration of LSD. They are not elicited by a variety of control drugs, but are elicited by other indole nucleus hallucinogens. Because the behavioral effects are specific, reliable, easy to score, and quantifiable, they represent an animal model that can be used in studies of the effects of LSD and related hallucinogens.", "PMID": 982043}
{"id": "pubmed23n0032_12886", "title": "Plasma membrane vesiculation: a new technique for isolation of plasma membranes.", "content": "Monolayer cell cultures of macrophages, monocytes, myoblasts, and density-inhibited and transformed fibroblasts form and release cell surface membrane vesicles following exposure to formaldehyde, related low-molecular-weight aldehydes, and disulfide blocking agents. Vesicles have a unique composition of proteins and lipids. They show enrichment of cholesterol and sphingomyelin content and a seven-to tenfold enrichment of 5'-nucleotidase activity. Vesicles also contain intramembranous particles and show a trilamellar unit membrane and no ultrastructural evidence of contamination with other cytoplasmic organelles. The technique is proposed as a novel method for isolating plasma membrane vesicles from cells in culture.", "contents": "Plasma membrane vesiculation: a new technique for isolation of plasma membranes. Monolayer cell cultures of macrophages, monocytes, myoblasts, and density-inhibited and transformed fibroblasts form and release cell surface membrane vesicles following exposure to formaldehyde, related low-molecular-weight aldehydes, and disulfide blocking agents. Vesicles have a unique composition of proteins and lipids. They show enrichment of cholesterol and sphingomyelin content and a seven-to tenfold enrichment of 5'-nucleotidase activity. Vesicles also contain intramembranous particles and show a trilamellar unit membrane and no ultrastructural evidence of contamination with other cytoplasmic organelles. The technique is proposed as a novel method for isolating plasma membrane vesicles from cells in culture.", "PMID": 982044}
{"id": "pubmed23n0032_12887", "title": "Karyotypic analysis and evidence of tetraploidy in the North American paddlefish, Polyodon spathula.", "content": "A model chromosome number of 120 was obtained for the ancient fish. Polyodon spathula (Pisces: Chondrostei). The karyotype consists of 48 macrochromosomes and 72 microchromosomes. The microchromosomes are like those found in certain other primitive fishes as well as in reptiles and birds. The possiblity that Polyodon is a species of tetraploid origin is strongly suggested by the fact that the 120 chromosomes are easily arranged into 30 groups of four homologs each. Evolutionary comparisons are made with other primitive fish groups.", "contents": "Karyotypic analysis and evidence of tetraploidy in the North American paddlefish, Polyodon spathula. A model chromosome number of 120 was obtained for the ancient fish. Polyodon spathula (Pisces: Chondrostei). The karyotype consists of 48 macrochromosomes and 72 microchromosomes. The microchromosomes are like those found in certain other primitive fishes as well as in reptiles and birds. The possiblity that Polyodon is a species of tetraploid origin is strongly suggested by the fact that the 120 chromosomes are easily arranged into 30 groups of four homologs each. Evolutionary comparisons are made with other primitive fish groups.", "PMID": 982045}
{"id": "pubmed23n0032_12888", "title": "Evidence of morphological and physiological transformation of mammalian cells by strong magnetic fields.", "content": "Cultures of L-929 and WI-38 cells, frozen to 4.2 degrees K and exposed for 4 to 8 hours to 5000-oersted magnetic fields, were markedly inhibited in their growth as compared to controls. In cultures grown on cover slips, approximately 7 days after exposure, morphologically distinct cells emerged and were propagated from generation to generation; 3 weeks later, in flask cultures, contact inhibition was abolished. It is concluded that under certain experimental conditions, strong magnetic fields induce morphological and physiological transformations of target cells.", "contents": "Evidence of morphological and physiological transformation of mammalian cells by strong magnetic fields. Cultures of L-929 and WI-38 cells, frozen to 4.2 degrees K and exposed for 4 to 8 hours to 5000-oersted magnetic fields, were markedly inhibited in their growth as compared to controls. In cultures grown on cover slips, approximately 7 days after exposure, morphologically distinct cells emerged and were propagated from generation to generation; 3 weeks later, in flask cultures, contact inhibition was abolished. It is concluded that under certain experimental conditions, strong magnetic fields induce morphological and physiological transformations of target cells.", "PMID": 982046}
{"id": "pubmed23n0032_12889", "title": "Thermal stability of human DNA and chimpanzee DNA heteroduplexes.", "content": "The base pairing fidelity of heteroduplexes formed from human DNA and chimpanzee DNA has been studied by the criterion of thermal stability to test the evolutionary conservation of repeated DNA base sequences.", "contents": "Thermal stability of human DNA and chimpanzee DNA heteroduplexes. The base pairing fidelity of heteroduplexes formed from human DNA and chimpanzee DNA has been studied by the criterion of thermal stability to test the evolutionary conservation of repeated DNA base sequences.", "PMID": 982047}
{"id": "pubmed23n0032_12890", "title": "Calcinogenic factor in Solanum malacoxylon: evidence that it is 1,25-dihydroxyvitamin D3-glycoside.", "content": "After glycosidic cleavage of the water-soluble vitamin D-like principle of the calcinogenic plant Solanum malacoxylon, the active lipophilic portion was purified by column chromatography and analyzed by combined gas chromatography and mass spectrometry. It was identified as 1,25-dihydroxyvitamin D3, the active form of vitamin D. Thus this active metabolite of vitamin D exists in the plant world, and its presence probably accounts for pathologic calcification in grazing animals ingesting Solanum malacoxylon.", "contents": "Calcinogenic factor in Solanum malacoxylon: evidence that it is 1,25-dihydroxyvitamin D3-glycoside. After glycosidic cleavage of the water-soluble vitamin D-like principle of the calcinogenic plant Solanum malacoxylon, the active lipophilic portion was purified by column chromatography and analyzed by combined gas chromatography and mass spectrometry. It was identified as 1,25-dihydroxyvitamin D3, the active form of vitamin D. Thus this active metabolite of vitamin D exists in the plant world, and its presence probably accounts for pathologic calcification in grazing animals ingesting Solanum malacoxylon.", "PMID": 982048}
{"id": "pubmed23n0032_12891", "title": "Radioimmunoassay for creatine kinase isoenzymes.", "content": "Creatine kinase has three isoenzymes designated MM, MB, and BB, with BB being the grain form and MM the muscle form. Antibodies to BB creatine kinase were obtained by immunization of rabbits with human BB creatine kinase. The antibodies demonstrated specificity for BB and MB creatine kinase (myocardial isoenzyme) but no cross-reactivity with MM creatine kinase. With the use of this antibody, a highly sensitive radioimmunoassay capable of measuring picomolar amounts of MB creatine kinase has been developed. Clinical application of this method should provide a sensitive and specific test for the diagnosis of myocardial infarction.", "contents": "Radioimmunoassay for creatine kinase isoenzymes. Creatine kinase has three isoenzymes designated MM, MB, and BB, with BB being the grain form and MM the muscle form. Antibodies to BB creatine kinase were obtained by immunization of rabbits with human BB creatine kinase. The antibodies demonstrated specificity for BB and MB creatine kinase (myocardial isoenzyme) but no cross-reactivity with MM creatine kinase. With the use of this antibody, a highly sensitive radioimmunoassay capable of measuring picomolar amounts of MB creatine kinase has been developed. Clinical application of this method should provide a sensitive and specific test for the diagnosis of myocardial infarction.", "PMID": 982049}
{"id": "pubmed23n0032_12892", "title": "Early eye removal produces excessive bilateral branching in the rat: application of cobalt filling method.", "content": "When one eye of a rat is removed at birth, axons from the remaining eye form an excess of branches which are directed to both sides of the brain. This finding, which is based on a novel application of cobalt tracing methods, provides an explanation for previous reports of expanded uncrossed projections after early eye removal.", "contents": "Early eye removal produces excessive bilateral branching in the rat: application of cobalt filling method. When one eye of a rat is removed at birth, axons from the remaining eye form an excess of branches which are directed to both sides of the brain. This finding, which is based on a novel application of cobalt tracing methods, provides an explanation for previous reports of expanded uncrossed projections after early eye removal.", "PMID": 982050}
{"id": "pubmed23n0032_12893", "title": "Risk of communicable disease infection associated with wastewater irrigation in agricultural settlements.", "content": "The incidence of enteric communicable diseases in 77 kibbutzim (agricultural communal settlements) practicing wastewater spray irrigation with partially treated nondisinfected oxidation pond effluent is compared with that in 130 kibbutzim practicing no form of wastewater irrigation. The incidence of shigellosis, salmonellosis, typhoid fever, and infectious hepatitis is two to four times higher in communities practicing wastewater irrigation. No significant differences are found for the incidence of streptococcal infections, tuberculosis, and laboratory-confirmed cases of influenza. Nor are differences found for enteric disease rates during the winter nonirrigation season. Strong wastewater treatment measures, including effective bacterial and viral inactivation through disinfection, are recommended for all cases of sewage irrigation or land disposal near residential areas in light of the potential public health risks involved.", "contents": "Risk of communicable disease infection associated with wastewater irrigation in agricultural settlements. The incidence of enteric communicable diseases in 77 kibbutzim (agricultural communal settlements) practicing wastewater spray irrigation with partially treated nondisinfected oxidation pond effluent is compared with that in 130 kibbutzim practicing no form of wastewater irrigation. The incidence of shigellosis, salmonellosis, typhoid fever, and infectious hepatitis is two to four times higher in communities practicing wastewater irrigation. No significant differences are found for the incidence of streptococcal infections, tuberculosis, and laboratory-confirmed cases of influenza. Nor are differences found for enteric disease rates during the winter nonirrigation season. Strong wastewater treatment measures, including effective bacterial and viral inactivation through disinfection, are recommended for all cases of sewage irrigation or land disposal near residential areas in light of the potential public health risks involved.", "PMID": 982051}
{"id": "pubmed23n0032_12894", "title": "Relaxin: a product of the human corpus luteum of pregnancy.", "content": "Plasma samples from peripheral and ovarian veins were obtains from women at cesarean section. A peptide that immunologically cross-reacts with a specific antiserum to porcine relaxin is present in all samples. Its concentration is four times higher in the ovarian vein draining the ovary, which contains the corpus luteum of pregnancy, than in either the peripheral vein or the contralateral ovarian vein. Secretion of ovarian relaxin correlates with secretion of ovarian progesterone, thus providing another index of luteal function.", "contents": "Relaxin: a product of the human corpus luteum of pregnancy. Plasma samples from peripheral and ovarian veins were obtains from women at cesarean section. A peptide that immunologically cross-reacts with a specific antiserum to porcine relaxin is present in all samples. Its concentration is four times higher in the ovarian vein draining the ovary, which contains the corpus luteum of pregnancy, than in either the peripheral vein or the contralateral ovarian vein. Secretion of ovarian relaxin correlates with secretion of ovarian progesterone, thus providing another index of luteal function.", "PMID": 982052}
{"id": "pubmed23n0032_12895", "title": "Bacillus thuringiensis enzyme-digested delta endotoxin: effect on cultured insect cells.", "content": "Cells from three insect cell lines responded to the enzyme-digested delta endotoxin of Bacillus thuringiensis with swelling, lysis, and vesicle formation. Sufficient toxin was taken up in 1 minute to cause half-maximal cell damage. Cytoxic activity was neutralized by specific antiserum to the endotoxin.", "contents": "Bacillus thuringiensis enzyme-digested delta endotoxin: effect on cultured insect cells. Cells from three insect cell lines responded to the enzyme-digested delta endotoxin of Bacillus thuringiensis with swelling, lysis, and vesicle formation. Sufficient toxin was taken up in 1 minute to cause half-maximal cell damage. Cytoxic activity was neutralized by specific antiserum to the endotoxin.", "PMID": 982053}
{"id": "pubmed23n0032_12896", "title": "Prenatal experience and avian development: brief auditory stimulation accelerates the hatching of Japanese quail.", "content": "A single 2-hour exposure to auditory stimulation at any point during the final 3 days of incubation accelerates the hatching of Japanese quail. The 3-day sensitive period includes both prenatal and perinatal stages of incubation. So far as is known these results provide the first unequivocal evidence that short-term prenatal sensory stimulation can affect the development of an avian embryo.", "contents": "Prenatal experience and avian development: brief auditory stimulation accelerates the hatching of Japanese quail. A single 2-hour exposure to auditory stimulation at any point during the final 3 days of incubation accelerates the hatching of Japanese quail. The 3-day sensitive period includes both prenatal and perinatal stages of incubation. So far as is known these results provide the first unequivocal evidence that short-term prenatal sensory stimulation can affect the development of an avian embryo.", "PMID": 982054}
{"id": "pubmed23n0032_12897", "title": "Trail marking by larvae of the eastern tent caterpillar.", "content": "Eastern tent caterpillars that are successful foragers deposit trails as they return to the tent that are more attractive than the exploratory trails of the unfed larvae. The trails of these fed returning larvae attract unfed tentmates to food finds anre chemical factors account for the attractiveness of these trails.", "contents": "Trail marking by larvae of the eastern tent caterpillar. Eastern tent caterpillars that are successful foragers deposit trails as they return to the tent that are more attractive than the exploratory trails of the unfed larvae. The trails of these fed returning larvae attract unfed tentmates to food finds anre chemical factors account for the attractiveness of these trails.", "PMID": 982055}
{"id": "pubmed23n0032_12898", "title": "A blind fish can school.", "content": "Vision is not required in order for fish to school. Five individual saithe, Pollachius virens, were able to join schools of 25 normal saithe swimming in an annular tank, while blinded with opaque eye covers. Test fish maintained position within the school indefinitely and responded to short-term movements of individuals within the school, although quantitative differences in reaction time and schooling behavior were noted. Five fish with lateral lines cut at the opercula were unable to school when wearing opaque eye covers. Although it is unlikely that blind saithe could school in the wild, the constraints of the apparatus permitted a demonstration of a role of the lateral line organ in schooling.", "contents": "A blind fish can school. Vision is not required in order for fish to school. Five individual saithe, Pollachius virens, were able to join schools of 25 normal saithe swimming in an annular tank, while blinded with opaque eye covers. Test fish maintained position within the school indefinitely and responded to short-term movements of individuals within the school, although quantitative differences in reaction time and schooling behavior were noted. Five fish with lateral lines cut at the opercula were unable to school when wearing opaque eye covers. Although it is unlikely that blind saithe could school in the wild, the constraints of the apparatus permitted a demonstration of a role of the lateral line organ in schooling.", "PMID": 982056}
{"id": "pubmed23n0032_12899", "title": "Cannabinoid induced behavioral convulsions in rabbits.", "content": "A population of New Zealand White rabbits has been found to exhibit behavioral convulsions when given low intravenous doses of psychoactive cannabinoids of marijuana. The behavioral convulsions decrease in severity and then disappear after the long-term administration of delta9-tetrahydrocannabinol. The extreme sensitivity of these rabbits to the stimulant action of cannabinoids suggests that the population might serve as a model in studies of the stimulant action of cannabinoids.", "contents": "Cannabinoid induced behavioral convulsions in rabbits. A population of New Zealand White rabbits has been found to exhibit behavioral convulsions when given low intravenous doses of psychoactive cannabinoids of marijuana. The behavioral convulsions decrease in severity and then disappear after the long-term administration of delta9-tetrahydrocannabinol. The extreme sensitivity of these rabbits to the stimulant action of cannabinoids suggests that the population might serve as a model in studies of the stimulant action of cannabinoids.", "PMID": 982057}
{"id": "pubmed23n0032_12900", "title": "Nitrous oxide \"analgesia\": resemblance to opiate action.", "content": "Nitrous oxide produced a dose-related \"analgesia\" in mice (median effective dose, 55 percent). The analgesia was evaluated by means of a phenylquinone writhing test. Narcotic antagonists or chronic morphinization reduced nitrous oxide analgesia. Either nitrous oxide releases an endogenous analgesic or narcotic antagonists have analgesic antagonist properties heretofore unappreciated.", "contents": "Nitrous oxide \"analgesia\": resemblance to opiate action. Nitrous oxide produced a dose-related \"analgesia\" in mice (median effective dose, 55 percent). The analgesia was evaluated by means of a phenylquinone writhing test. Narcotic antagonists or chronic morphinization reduced nitrous oxide analgesia. Either nitrous oxide releases an endogenous analgesic or narcotic antagonists have analgesic antagonist properties heretofore unappreciated.", "PMID": 982058}
{"id": "pubmed23n0032_12901", "title": "Effect of charcoal-broiled beef on phenacetin metabolism in man.", "content": "When charcoal-broiled beef was fed to human volunteers, who were then given phenacetin orally, the concentration of phenacetin in the plasma was lowered, but its half-life in the plasma was not changed. The data suggest that feeding charcoal-broiled beef enhances the metabolism of orally administered phenacetin in the intestine or during its first pass through the liver, or both.", "contents": "Effect of charcoal-broiled beef on phenacetin metabolism in man. When charcoal-broiled beef was fed to human volunteers, who were then given phenacetin orally, the concentration of phenacetin in the plasma was lowered, but its half-life in the plasma was not changed. The data suggest that feeding charcoal-broiled beef enhances the metabolism of orally administered phenacetin in the intestine or during its first pass through the liver, or both.", "PMID": 982059}
{"id": "pubmed23n0032_12902", "title": "Impurities in labeled diethylstilbestrol: identification of pseudodiethylstilbestrol.", "content": "Radioactive impurities known to exist in labeled diethylstilbestrol used in tissue residue studies in cattle include at least five different compounds, one of which is identified as pseudodiethylstilbestrol.", "contents": "Impurities in labeled diethylstilbestrol: identification of pseudodiethylstilbestrol. Radioactive impurities known to exist in labeled diethylstilbestrol used in tissue residue studies in cattle include at least five different compounds, one of which is identified as pseudodiethylstilbestrol.", "PMID": 982060}
{"id": "pubmed23n0032_12903", "title": "Interindividual variation in binding of benzo[a]pyrene to DNA in cultured human bronchi.", "content": "The binding of benzo[a]pyrene to DNA in cultured human bronchus was measured in specimens from 37 patients. The binding values ranged from 2 to 151 picomoles of benzo[a]pyrene per milligram of DNA with an overall mean +/- standard error of 34.2 +/- 5.2. This 75-fold interindividual variation in the binding of benzo[a]pyrene to DNA is similar in magnitude to that found in pharmacogenetic studies of drug metabolism. Aryl hydrocarbon hydroxylase is also inducible by benz[a]anthracene in the bronchial mucosa.", "contents": "Interindividual variation in binding of benzo[a]pyrene to DNA in cultured human bronchi. The binding of benzo[a]pyrene to DNA in cultured human bronchus was measured in specimens from 37 patients. The binding values ranged from 2 to 151 picomoles of benzo[a]pyrene per milligram of DNA with an overall mean +/- standard error of 34.2 +/- 5.2. This 75-fold interindividual variation in the binding of benzo[a]pyrene to DNA is similar in magnitude to that found in pharmacogenetic studies of drug metabolism. Aryl hydrocarbon hydroxylase is also inducible by benz[a]anthracene in the bronchial mucosa.", "PMID": 982061}
{"id": "pubmed23n0032_12904", "title": "25-hydroxyvitamin D: autoradiographic evidence of sites of action in epiphyseal cartilage and bone.", "content": "Tritiated 25-hydroxyvitamin D3 was administered to growing rats to morphologically documents its sites of action. Highly selective incorporation occurred in epiphyseal hypertrophic cells, epiphyseal matrix, osteoid, osteoblasts, and osteocytes of metaphyseal bone spicules. The labeled metabolite appeared in chondrocytic lacunar matrix coincident with hypertrophic cell death as evidenced by histological examination. The tritiated 25-hydroxyvitamin D3 became localized only in areas of active mineralization.", "contents": "25-hydroxyvitamin D: autoradiographic evidence of sites of action in epiphyseal cartilage and bone. Tritiated 25-hydroxyvitamin D3 was administered to growing rats to morphologically documents its sites of action. Highly selective incorporation occurred in epiphyseal hypertrophic cells, epiphyseal matrix, osteoid, osteoblasts, and osteocytes of metaphyseal bone spicules. The labeled metabolite appeared in chondrocytic lacunar matrix coincident with hypertrophic cell death as evidenced by histological examination. The tritiated 25-hydroxyvitamin D3 became localized only in areas of active mineralization.", "PMID": 982062}
{"id": "pubmed23n0032_12905", "title": "Rod outer segment disk shedding in rat retina: relationship to cyclic lighting.", "content": "When albino rats are reared in cyclic light, a burst of rod outer segment disk shedding occurs in the retina soon after the onset of light. The number of large packets of outer segment disks (phagosomes) in the pigment epithelium at this time is 2.5 to 5 times greater than at any other time of day or night. The subsequent degradation of large phagosomes to smaller structures within pigment epithelial cells proceeds rapidly. The burst of disk shedding follows a circadian rhythm for at least 3 days, since it occurs in continuous darkness at the same time without the onset of light.", "contents": "Rod outer segment disk shedding in rat retina: relationship to cyclic lighting. When albino rats are reared in cyclic light, a burst of rod outer segment disk shedding occurs in the retina soon after the onset of light. The number of large packets of outer segment disks (phagosomes) in the pigment epithelium at this time is 2.5 to 5 times greater than at any other time of day or night. The subsequent degradation of large phagosomes to smaller structures within pigment epithelial cells proceeds rapidly. The burst of disk shedding follows a circadian rhythm for at least 3 days, since it occurs in continuous darkness at the same time without the onset of light.", "PMID": 982063}
{"id": "pubmed23n0032_12906", "title": "Time-dependent disruption of morphine tolerance by electroconvulsive shock and frontal cortical stimulation.", "content": "Electroconvulsive shock or frontal cortex stimulation administered to rats at 5 but not at 180 minutes after an initial administration of morphine sulfate disrupted the development of one-trial tolerance to the analgesic effects of morphine sulfate. It is suggested that development of tolerance may be mediated by cellular mechanisms and memory processes similar to those thought to underlie conventional learning.", "contents": "Time-dependent disruption of morphine tolerance by electroconvulsive shock and frontal cortical stimulation. Electroconvulsive shock or frontal cortex stimulation administered to rats at 5 but not at 180 minutes after an initial administration of morphine sulfate disrupted the development of one-trial tolerance to the analgesic effects of morphine sulfate. It is suggested that development of tolerance may be mediated by cellular mechanisms and memory processes similar to those thought to underlie conventional learning.", "PMID": 982064}
{"id": "pubmed23n0032_12907", "title": "Perceptual analysis of moving patterns.", "content": "Configurations of moving points are often perceptually analyzed into relative and common vectors that are different from the actual motions. If a movement configuration is abruptly replaced by a test point whose objective velocity continues the apparent (but illusory) course of one of the original points, observers perceive that course as uninterrupted and colinear. This finding provides a quantitative measure of the vector extraction phenomenon and was used to show that neither of the two current models adequately fits that phenomenon.", "contents": "Perceptual analysis of moving patterns. Configurations of moving points are often perceptually analyzed into relative and common vectors that are different from the actual motions. If a movement configuration is abruptly replaced by a test point whose objective velocity continues the apparent (but illusory) course of one of the original points, observers perceive that course as uninterrupted and colinear. This finding provides a quantitative measure of the vector extraction phenomenon and was used to show that neither of the two current models adequately fits that phenomenon.", "PMID": 982065}
{"id": "pubmed23n0032_12908", "title": "Experimental models for evaluation of radioactive tumor-localizing agents.", "content": "Although subject to limitations, there is a need for carefully controlled laboratory studies using animal tumor models in research on tumor-localizing agents. This paper reviews the literature relating to the more important transplantable tumor systems, spontaneous or induced, as to origin, host, site, and radioactive agent used. The historical background of animal tumor models is discussed, including such technical aspects as source of tumor, techniques of transplantation, transplantation sites, and maintenance of transplants. Also, considered are the use of animal tumor models as predictive systems, expression of experimental results of quantitative studies of tissue uptake and comparative radionuclide tumor and distributional studies, and suggestions for future studies, such as the need of more intensive study of existing tumor models for a better understanding of the relationship between animal and human tumors, the need for the development of new tumor model systems, and for standardization of experimental protocols and procedures. A total of 48 t,mor models (26 in mice, 11 in rats, 7 in hamsters, 2 in rabbits, and 2 in dogs) are presented in seven tables dividing the models into tumors of epithelial tissue, connective tissue, hematopoietic tissues, melanin-forming tissue, neural tissues, undetermined site of origin or undifferentiated histologic pattern, and miscellaneous background. The use of animal tumor models in cancer research, which utilizes radionuclides, permits the investigator to do many things not permissible with human beings, but the extrapolation of animal results to human beings must be approached with caution, Although malignant disease, whether in animals or man, must be individualized, certain trends in animal studies can be observed. It is the goal of the laboratory investigator to be able to indicate to the clinician those trends or phenomena that, when repeatedly observed in animal model systems, may be applicable to an understanding of malignant disease in man.", "contents": "Experimental models for evaluation of radioactive tumor-localizing agents. Although subject to limitations, there is a need for carefully controlled laboratory studies using animal tumor models in research on tumor-localizing agents. This paper reviews the literature relating to the more important transplantable tumor systems, spontaneous or induced, as to origin, host, site, and radioactive agent used. The historical background of animal tumor models is discussed, including such technical aspects as source of tumor, techniques of transplantation, transplantation sites, and maintenance of transplants. Also, considered are the use of animal tumor models as predictive systems, expression of experimental results of quantitative studies of tissue uptake and comparative radionuclide tumor and distributional studies, and suggestions for future studies, such as the need of more intensive study of existing tumor models for a better understanding of the relationship between animal and human tumors, the need for the development of new tumor model systems, and for standardization of experimental protocols and procedures. A total of 48 t,mor models (26 in mice, 11 in rats, 7 in hamsters, 2 in rabbits, and 2 in dogs) are presented in seven tables dividing the models into tumors of epithelial tissue, connective tissue, hematopoietic tissues, melanin-forming tissue, neural tissues, undetermined site of origin or undifferentiated histologic pattern, and miscellaneous background. The use of animal tumor models in cancer research, which utilizes radionuclides, permits the investigator to do many things not permissible with human beings, but the extrapolation of animal results to human beings must be approached with caution, Although malignant disease, whether in animals or man, must be individualized, certain trends in animal studies can be observed. It is the goal of the laboratory investigator to be able to indicate to the clinician those trends or phenomena that, when repeatedly observed in animal model systems, may be applicable to an understanding of malignant disease in man.", "PMID": 982075}
{"id": "pubmed23n0032_12909", "title": "Mechanisms for localization of radiopharmaceuticals in neoplasms.", "content": "Localization of a radiopharmaceutical agent in a \"tumor\" is best conceptualized in terms of the altered regional physiology attendant to the presence of the \"tumor\". Such localization should be expected to occur in association with other disease states characterized by similar altered regional physiology. Neoplasms, areas of inflammation, and certain phases of infarct development are characterized by increased permeability of their capillary beds to macromolecules. This is largely due to neovascularization and the large intercapillary pores associated with new growth of capillary beds in these circumstances. Often, total perfusion to such lesions is increased in comparison to surrounding normal tissue. Thus, in all three clinical conditions, the entry of macro-molecules into the interstitial fluid space from the intravascular space is increased above that seen in normal tissue. Moreover, with neoplasms and inflammatory processes, there may be a delay in new lymphatic vessel growth adding to the residence time of the macromolecules in the interstitial fluid space. In all three conditions, the increased macrophage activity associated with tissue necrosis may result in ingestion of the labeled macromolecule by the macrophage. Pinocytosis may result in ingestion of the labeled macromolecule by other cells in the lesion, or there may be specific receptor sites on the cell membrane for the macromolecule, which may lead to fixation of the labeled macromolecule on the cell surface and possible intracellular translocation of the label itself. Radiolabeled macromolecules such as albumin, fibrinogen, or gamma globulins, and radionuclides that bind to macromolecules such as radiogallium, radioindium, and other radioelements, exhibit localizing behavior in tumors, inflammatory lesions, and during certain stages of infarcts. In the case of radiogallium and radioindium, the binding macromolecule is transferrin, and it is known that some cells have specific receptor sites for transferrin-bound iron on the cell membrane. It is possible that certain cells within these lesions have cell membrane receptor sites for radiogallium- and radioindium-labeled transferrin, and the cell erroneously accepts these radioelements from the transferrin in lieu of iron in attempting to engage in heme enzyme synthesis. Another mechanism that may be operative in the localization of agents in neoplasms, infarcts, and inflammatory lesions may be the altered cell permeability found in many cells of such lesions. It is known that many agents, such as supravital dyes, are excluded from entering normal cells by the selective permeability of normal cell membranes. When cell membrane permeability is altered, such as can be seen in traumatized, dying, or dead cells, the normally excluded agent may penetrate the abnormal cell membrane and bind, and consequently accumulate in intracellular constituents.", "contents": "Mechanisms for localization of radiopharmaceuticals in neoplasms. Localization of a radiopharmaceutical agent in a \"tumor\" is best conceptualized in terms of the altered regional physiology attendant to the presence of the \"tumor\". Such localization should be expected to occur in association with other disease states characterized by similar altered regional physiology. Neoplasms, areas of inflammation, and certain phases of infarct development are characterized by increased permeability of their capillary beds to macromolecules. This is largely due to neovascularization and the large intercapillary pores associated with new growth of capillary beds in these circumstances. Often, total perfusion to such lesions is increased in comparison to surrounding normal tissue. Thus, in all three clinical conditions, the entry of macro-molecules into the interstitial fluid space from the intravascular space is increased above that seen in normal tissue. Moreover, with neoplasms and inflammatory processes, there may be a delay in new lymphatic vessel growth adding to the residence time of the macromolecules in the interstitial fluid space. In all three conditions, the increased macrophage activity associated with tissue necrosis may result in ingestion of the labeled macromolecule by the macrophage. Pinocytosis may result in ingestion of the labeled macromolecule by other cells in the lesion, or there may be specific receptor sites on the cell membrane for the macromolecule, which may lead to fixation of the labeled macromolecule on the cell surface and possible intracellular translocation of the label itself. Radiolabeled macromolecules such as albumin, fibrinogen, or gamma globulins, and radionuclides that bind to macromolecules such as radiogallium, radioindium, and other radioelements, exhibit localizing behavior in tumors, inflammatory lesions, and during certain stages of infarcts. In the case of radiogallium and radioindium, the binding macromolecule is transferrin, and it is known that some cells have specific receptor sites for transferrin-bound iron on the cell membrane. It is possible that certain cells within these lesions have cell membrane receptor sites for radiogallium- and radioindium-labeled transferrin, and the cell erroneously accepts these radioelements from the transferrin in lieu of iron in attempting to engage in heme enzyme synthesis. Another mechanism that may be operative in the localization of agents in neoplasms, infarcts, and inflammatory lesions may be the altered cell permeability found in many cells of such lesions. It is known that many agents, such as supravital dyes, are excluded from entering normal cells by the selective permeability of normal cell membranes. When cell membrane permeability is altered, such as can be seen in traumatized, dying, or dead cells, the normally excluded agent may penetrate the abnormal cell membrane and bind, and consequently accumulate in intracellular constituents.", "PMID": 982076}
{"id": "pubmed23n0032_12910", "title": "The relationship of head and neck irradiation to the subsequent development of thyroid neoplasms.", "content": "Individuals who have received head and neck radiation for benign conditions have a markedly increased risk of developing thyroid, salivary, and perhaps breast cancer as compared to the general population. Although the relative risk is very high, the absolute risk that any one individual who has had head or neck irradiation will develop a subsequent malignancy is low. Identification of these patients through some type of screening procedure may be beneficial in terms of prevention of subsequent morbidity and perhaps mortality from cancer, especially thyroid and salivary cancer. The risks of any detection or prophylaxis program must be carefully weighed against the probable, but unproved benefits of early detection. A major unresolved question is the natural history of microscopic thyroid carcinoma in the 25 yr-40 yr old radiation exposed population.", "contents": "The relationship of head and neck irradiation to the subsequent development of thyroid neoplasms. Individuals who have received head and neck radiation for benign conditions have a markedly increased risk of developing thyroid, salivary, and perhaps breast cancer as compared to the general population. Although the relative risk is very high, the absolute risk that any one individual who has had head or neck irradiation will develop a subsequent malignancy is low. Identification of these patients through some type of screening procedure may be beneficial in terms of prevention of subsequent morbidity and perhaps mortality from cancer, especially thyroid and salivary cancer. The risks of any detection or prophylaxis program must be carefully weighed against the probable, but unproved benefits of early detection. A major unresolved question is the natural history of microscopic thyroid carcinoma in the 25 yr-40 yr old radiation exposed population.", "PMID": 982077}
{"id": "pubmed23n0032_12911", "title": "Tobacco and tobacco smoke.", "content": "Epidemiologic studies have demonstrated a causal relation between smoking of cigarettes and cancer of the lung in man. Women smokers, cigar, and pipe smokers also face an increased risk for lung cancer. Prospective and retrospective studies have found a correlation between smoking of cigarettes, cigars, and pipes and cancer of the oral cavity, larynx, and esophagus and for cigarette smokers increased risks to develop cancer of the pancreas, kidney, and urinary bladder. Dose responses have been established between number of cigarettes smoked and cancer of the respiratory and upper digestive tract. Tobacco chewers face an increased risk for cancer of the mouth and esophagus. Tobacco smoke has induced tumors of the lung in the dogs and of the larynx of hamsters. The particulate matter of the smoke is carcinogenic to the skin of mice and rabbits, and the bronchi and connective tissue of rats. In tobacco smoke were identified tumor initiators, tumor promoters, cocarcinogens and organ specific carcinogens. Chewing tobacco is a tumor promoting agent and contains traces of tobacco specific and carcinogenic nitrosamines. Ten to 15 yr after giving up smoking the ex-smoker faces the same low risk to develop cancer of the upper digestive tract, the lung, the pancreas, and the urinary tract as the nonsmoker. It should be our goal, therefore, to prevent young people from starting the smoking habit and to convince the smoker to quit smoking. So far, we can report no success in terms of decreasing smoking habits among younger people. On the other hand, we can take satisfaction from the fact that antismoking propaganda has had an effect on college educated males, that among the population as a whole, there is a considerable number of exsmokers; that smoking cessation clinics do prove cost effective and if they were to become part of every health care center, they could help a large number of heavy smokers who cannot seem to stop smoking on their own. We can also report that there has been a significant reduction in the tar yield of American cigarettes, a reduction which we hope will continue; that the tumorigenic activity of tobacco as measured in animal studies, has decreased; and that as a consequence of the above, the risk of lung cancer and other tobacco-related cancers among smokers of these cigarettes is lower than in years past. It is unlikely that man will ever be able to inhale smoke components as harmless as unpolluted air, but as long as we have a society which accepts this habit and as long as people find satisfaction in smoking, we must work towards the day when tobacco-related cancers and other diseases will be reduced to a minimum. With the world wide coperation of the scientific community, the Departments of Agriculture, and the tobacco industry, it is our hope that this goal will be achieved.", "contents": "Tobacco and tobacco smoke. Epidemiologic studies have demonstrated a causal relation between smoking of cigarettes and cancer of the lung in man. Women smokers, cigar, and pipe smokers also face an increased risk for lung cancer. Prospective and retrospective studies have found a correlation between smoking of cigarettes, cigars, and pipes and cancer of the oral cavity, larynx, and esophagus and for cigarette smokers increased risks to develop cancer of the pancreas, kidney, and urinary bladder. Dose responses have been established between number of cigarettes smoked and cancer of the respiratory and upper digestive tract. Tobacco chewers face an increased risk for cancer of the mouth and esophagus. Tobacco smoke has induced tumors of the lung in the dogs and of the larynx of hamsters. The particulate matter of the smoke is carcinogenic to the skin of mice and rabbits, and the bronchi and connective tissue of rats. In tobacco smoke were identified tumor initiators, tumor promoters, cocarcinogens and organ specific carcinogens. Chewing tobacco is a tumor promoting agent and contains traces of tobacco specific and carcinogenic nitrosamines. Ten to 15 yr after giving up smoking the ex-smoker faces the same low risk to develop cancer of the upper digestive tract, the lung, the pancreas, and the urinary tract as the nonsmoker. It should be our goal, therefore, to prevent young people from starting the smoking habit and to convince the smoker to quit smoking. So far, we can report no success in terms of decreasing smoking habits among younger people. On the other hand, we can take satisfaction from the fact that antismoking propaganda has had an effect on college educated males, that among the population as a whole, there is a considerable number of exsmokers; that smoking cessation clinics do prove cost effective and if they were to become part of every health care center, they could help a large number of heavy smokers who cannot seem to stop smoking on their own. We can also report that there has been a significant reduction in the tar yield of American cigarettes, a reduction which we hope will continue; that the tumorigenic activity of tobacco as measured in animal studies, has decreased; and that as a consequence of the above, the risk of lung cancer and other tobacco-related cancers among smokers of these cigarettes is lower than in years past. It is unlikely that man will ever be able to inhale smoke components as harmless as unpolluted air, but as long as we have a society which accepts this habit and as long as people find satisfaction in smoking, we must work towards the day when tobacco-related cancers and other diseases will be reduced to a minimum. With the world wide coperation of the scientific community, the Departments of Agriculture, and the tobacco industry, it is our hope that this goal will be achieved.", "PMID": 982079}
{"id": "pubmed23n0032_12912", "title": "High school football injuries: prevention of injury to the central nervous system.", "content": "Injuries to the head, neck, and spinal cord of high school athletes account for over 90% of the fatalities directly related to football. Preseasonal and seasonal guidelines that have proven to be helpful in the prevention of these injuries are presented.", "contents": "High school football injuries: prevention of injury to the central nervous system. Injuries to the head, neck, and spinal cord of high school athletes account for over 90% of the fatalities directly related to football. Preseasonal and seasonal guidelines that have proven to be helpful in the prevention of these injuries are presented.", "PMID": 982098}
{"id": "pubmed23n0032_12913", "title": "Harrington instrumentation in fractures and dislocations of the thoracic and lumbar spine.", "content": "Harrington instrumentation was used to treat 23 injuries of the thoracic and lumbar spine accompanied by neural damage encountered during an 8 1/2-year period. The indication for the use of Harrington instrumentation is instability of the thoracic or lumbar spine due to trauma, laminectomy, or both. Of the 13 patients with incomplete neural lesions in the series, 12 improved. One of these was a cauda equina lesion. No patient in the series was neurologically worse after operation, in contrast to other reported series in which laminectomy alone was done. It is concluded that Harrington instrumentation is the strongest available system of internal fixation, and the only one that permits reduction of nearly all traumatic spinal deformities at the time of operation.", "contents": "Harrington instrumentation in fractures and dislocations of the thoracic and lumbar spine. Harrington instrumentation was used to treat 23 injuries of the thoracic and lumbar spine accompanied by neural damage encountered during an 8 1/2-year period. The indication for the use of Harrington instrumentation is instability of the thoracic or lumbar spine due to trauma, laminectomy, or both. Of the 13 patients with incomplete neural lesions in the series, 12 improved. One of these was a cauda equina lesion. No patient in the series was neurologically worse after operation, in contrast to other reported series in which laminectomy alone was done. It is concluded that Harrington instrumentation is the strongest available system of internal fixation, and the only one that permits reduction of nearly all traumatic spinal deformities at the time of operation.", "PMID": 982099}
{"id": "pubmed23n0032_12914", "title": "Usefulness of serial carcinoembryonic antigen (CEA) determinations in monitoring chemotherapy.", "content": "Serial carcinoembryonic antigen (CEA) levels were measured during chemotherapy for metastatic cancer in 94 patients. Criteria for chemotherapy responses were those used by the Central Oncology Group. Patients were classified according to changes in CEA levels and response to chemotherapy. Four categories represented a positive correlation: (1) increasing abnormal CEA with progressing disease, (2) decreasing abnormal CEA with disease regression, (3) unchanged abnormal CEA with stable disease, (4) change from normal to abnormal CEA with progressive disease. Positive correlation of serial CEA levels with clinical responses occurred in 71% of patients with GI cancer, 51% with breast cancer, 42% with sarcoma, 50% with respiratory cancer, and 25% with melanoma. These data indicate that serial CEA determinations may be of value as an additional parameter of response to chemotherapy in gastrointestinal cancer.", "contents": "Usefulness of serial carcinoembryonic antigen (CEA) determinations in monitoring chemotherapy. Serial carcinoembryonic antigen (CEA) levels were measured during chemotherapy for metastatic cancer in 94 patients. Criteria for chemotherapy responses were those used by the Central Oncology Group. Patients were classified according to changes in CEA levels and response to chemotherapy. Four categories represented a positive correlation: (1) increasing abnormal CEA with progressing disease, (2) decreasing abnormal CEA with disease regression, (3) unchanged abnormal CEA with stable disease, (4) change from normal to abnormal CEA with progressive disease. Positive correlation of serial CEA levels with clinical responses occurred in 71% of patients with GI cancer, 51% with breast cancer, 42% with sarcoma, 50% with respiratory cancer, and 25% with melanoma. These data indicate that serial CEA determinations may be of value as an additional parameter of response to chemotherapy in gastrointestinal cancer.", "PMID": 982100}
{"id": "pubmed23n0032_12915", "title": "Current management of pelvic fractures: a combined surgical-angiographic approach to hemorrhage.", "content": "Pelvic retroperitoneal hemorrhage after pelvic fracture accounts for the high mortality and morbidity associated with this injury, a concept confirmed by analysis of 357 patients with pelvic fractures at the University of Kentucky Medical Center. The overall mortality of 9.8% was almost exclusively limited to patients with unstable fractures or crush injuries. The reliability of diagnostic peritoneal lavage in excluding associated intraperitoneal injury and the recognized utility of angiographic methods in identifying the site of bleeding constitute a major advance in management. Prompt angiographic definition of injury to major arterial trunks can lead to early operative intervention for direct vascular control. Bleeding from branches of the hypogastric artery can be managed effectively by clot embolization technic, avoiding operation. Venous bleeding is best treated by tamponade in most cases.", "contents": "Current management of pelvic fractures: a combined surgical-angiographic approach to hemorrhage. Pelvic retroperitoneal hemorrhage after pelvic fracture accounts for the high mortality and morbidity associated with this injury, a concept confirmed by analysis of 357 patients with pelvic fractures at the University of Kentucky Medical Center. The overall mortality of 9.8% was almost exclusively limited to patients with unstable fractures or crush injuries. The reliability of diagnostic peritoneal lavage in excluding associated intraperitoneal injury and the recognized utility of angiographic methods in identifying the site of bleeding constitute a major advance in management. Prompt angiographic definition of injury to major arterial trunks can lead to early operative intervention for direct vascular control. Bleeding from branches of the hypogastric artery can be managed effectively by clot embolization technic, avoiding operation. Venous bleeding is best treated by tamponade in most cases.", "PMID": 982101}
{"id": "pubmed23n0032_12916", "title": "Practical uses of a quantitative renal scintillation camera study.", "content": "A quantitative renal scintillation camera study has been described for the evaluation of total renal function and the split function of the two kidneys by a noninvasive technic. It requires no patient preparation, is not affected by bowel gas, and no allergic reactions have been reported. Six cases are reported to illustrate its usefulness with renal tumors, neurogenic bladders, pyelonephritis, nephrolithiasis, congenital anomalies, and renal insufficiency.", "contents": "Practical uses of a quantitative renal scintillation camera study. A quantitative renal scintillation camera study has been described for the evaluation of total renal function and the split function of the two kidneys by a noninvasive technic. It requires no patient preparation, is not affected by bowel gas, and no allergic reactions have been reported. Six cases are reported to illustrate its usefulness with renal tumors, neurogenic bladders, pyelonephritis, nephrolithiasis, congenital anomalies, and renal insufficiency.", "PMID": 982102}
{"id": "pubmed23n0032_12917", "title": "Anesthesia for EMI scanning in infants and small children.", "content": "Computerized transverse axial scanning is a new, noninvasive method of diagnosing intracranial and orbital lesions. It is mandatory that the patient's head be motionless throughout the scanning procedure. This requirement creates problems in managing infants and small children for the scanning process. Ketamine hydrochloride was given intramuscularly to produce the desired motionless stated in 47 consecutive infants and small children. Satisfactory scans were obtained in all except two cases. Contraindications and precautions to be observed using this technic are discussed. It is believed that intramuscularly administered ketamine is a safe, effective means of providing immobilization for EMI scanning in infants and small children who are normotensive and show no evidence of definitely increased intracranial pressure.", "contents": "Anesthesia for EMI scanning in infants and small children. Computerized transverse axial scanning is a new, noninvasive method of diagnosing intracranial and orbital lesions. It is mandatory that the patient's head be motionless throughout the scanning procedure. This requirement creates problems in managing infants and small children for the scanning process. Ketamine hydrochloride was given intramuscularly to produce the desired motionless stated in 47 consecutive infants and small children. Satisfactory scans were obtained in all except two cases. Contraindications and precautions to be observed using this technic are discussed. It is believed that intramuscularly administered ketamine is a safe, effective means of providing immobilization for EMI scanning in infants and small children who are normotensive and show no evidence of definitely increased intracranial pressure.", "PMID": 982103}
{"id": "pubmed23n0032_12918", "title": "The Ocusert pilocarpine system: advantages and disadvantages.", "content": "Thirty-four patients with glaucoma were treated with the Ocusert pilocarpine system, a unique method of providing round-the-clock delivery of pilocarpine in the eye for seven-day periods. Advantages of the device include therapeutic effectiveness and continuous release rate, less effect on accommodation, less miosis, convenience for the patient, and reliability in patients who must rely on others for treatment (eg, children and the elderly). Some disadvantages encountered were need for instruction and encouragement of patients; retention difficulties, with some patients unaware when the device was lost; occasional side effects of a cutting sensation (as when the Ocusert sometimes doubles over), movement in the eye, and transient blurring of vision and miosis; and high cost. Overall, when compared to pilocarpine eyedrops, the Ocusert pilocarpine system presents many definite advantages and is a highly desirable method of therapy in selected cases of glaucoma.", "contents": "The Ocusert pilocarpine system: advantages and disadvantages. Thirty-four patients with glaucoma were treated with the Ocusert pilocarpine system, a unique method of providing round-the-clock delivery of pilocarpine in the eye for seven-day periods. Advantages of the device include therapeutic effectiveness and continuous release rate, less effect on accommodation, less miosis, convenience for the patient, and reliability in patients who must rely on others for treatment (eg, children and the elderly). Some disadvantages encountered were need for instruction and encouragement of patients; retention difficulties, with some patients unaware when the device was lost; occasional side effects of a cutting sensation (as when the Ocusert sometimes doubles over), movement in the eye, and transient blurring of vision and miosis; and high cost. Overall, when compared to pilocarpine eyedrops, the Ocusert pilocarpine system presents many definite advantages and is a highly desirable method of therapy in selected cases of glaucoma.", "PMID": 982104}
{"id": "pubmed23n0032_12919", "title": "Gallbladder disease in patients 20 years of age and under.", "content": "Cholecystitis is uncommon in individuals under 21 years of age and, when present, usually is associated with pregnancy, sepsis, metabolic defects, hemolytic anemia, or hereditary disorders. Over the past 4 1/2 years, 92 patients 20 years of age and younger have been admitted to our institution with gallbadder disease. Of these patients, 88 were female and 76 were of Mexican-American origin. Only two of these individuals had associated hemolytic anemia. Seventy-six of these females had been pregnant at least once, and 57 were more than 10 pound overweight. Cholecystectomy was done on 88 of these patients, and cholelithiasis was found in 86. There were 28 explorations of the common bile duct, 18 of which were positive. This series represents an earlier onset of cholelithiasis in this population.", "contents": "Gallbladder disease in patients 20 years of age and under. Cholecystitis is uncommon in individuals under 21 years of age and, when present, usually is associated with pregnancy, sepsis, metabolic defects, hemolytic anemia, or hereditary disorders. Over the past 4 1/2 years, 92 patients 20 years of age and younger have been admitted to our institution with gallbadder disease. Of these patients, 88 were female and 76 were of Mexican-American origin. Only two of these individuals had associated hemolytic anemia. Seventy-six of these females had been pregnant at least once, and 57 were more than 10 pound overweight. Cholecystectomy was done on 88 of these patients, and cholelithiasis was found in 86. There were 28 explorations of the common bile duct, 18 of which were positive. This series represents an earlier onset of cholelithiasis in this population.", "PMID": 982105}
{"id": "pubmed23n0032_12920", "title": "Lens surgery with the vitreous infusion suction cutter.", "content": "The vitreous infusion suction cutter was used in the removal of congenital and adult cataracts or lens remnants. The instrument may be used through a standard corneoscleral incision or through the pars plana. When a hard nucleus cannot be dispersed, ultrasonic phacofragmentation may be of help. Several brief case illustrations demonstrate the effectiveness of this instrument in lens surgery.", "contents": "Lens surgery with the vitreous infusion suction cutter. The vitreous infusion suction cutter was used in the removal of congenital and adult cataracts or lens remnants. The instrument may be used through a standard corneoscleral incision or through the pars plana. When a hard nucleus cannot be dispersed, ultrasonic phacofragmentation may be of help. Several brief case illustrations demonstrate the effectiveness of this instrument in lens surgery.", "PMID": 982106}
{"id": "pubmed23n0032_12921", "title": "An antacid tasting: the relative palatability of 19 liquid antacids.", "content": "The taste of liquid antacids is proposed as a major factor in patient compliance in taking such medication when prescribed. A study was made of the relative palatability of 19 antacids. The antacids can be divided into three groups of varying palatability (p less than 0.05).", "contents": "An antacid tasting: the relative palatability of 19 liquid antacids. The taste of liquid antacids is proposed as a major factor in patient compliance in taking such medication when prescribed. A study was made of the relative palatability of 19 antacids. The antacids can be divided into three groups of varying palatability (p less than 0.05).", "PMID": 982107}
{"id": "pubmed23n0032_12922", "title": "Five-year retrospective study of hospitalization and treatment of patients with sickle cell anemia.", "content": "Examination of computerized hospital records of 463 admissions of 255 patients with sickle cell anemia was made. Patients ranged in age from infancy to 65, with a mean age of 17-7 years. Only 119 of the 255 patients were male, but 54% of the admissions were for males. Most patients were admitted to pediatrics and internal medicine services. Fluids and blood derivatives were given in over 60% of cases and anti-infection drugs in 40%. Mean hospital stay was 14.75 days, twice the mean for other patients. Thirty-six percent of the patients were admitted more than once. Mortality was 1.3% for the 463 admissions. Blue Cross or Medicaid accounted for payment in 51% of admissions. This information can be helpful to health planners providing comprehensive services for sickle cell patients.", "contents": "Five-year retrospective study of hospitalization and treatment of patients with sickle cell anemia. Examination of computerized hospital records of 463 admissions of 255 patients with sickle cell anemia was made. Patients ranged in age from infancy to 65, with a mean age of 17-7 years. Only 119 of the 255 patients were male, but 54% of the admissions were for males. Most patients were admitted to pediatrics and internal medicine services. Fluids and blood derivatives were given in over 60% of cases and anti-infection drugs in 40%. Mean hospital stay was 14.75 days, twice the mean for other patients. Thirty-six percent of the patients were admitted more than once. Mortality was 1.3% for the 463 admissions. Blue Cross or Medicaid accounted for payment in 51% of admissions. This information can be helpful to health planners providing comprehensive services for sickle cell patients.", "PMID": 982108}
{"id": "pubmed23n0032_12923", "title": "Fantasy and self-esteem of renal dialysis patients and their spouses.", "content": "The fantasy life and self-esteem of 22 male and 13 female renal dialysis patients and their spouses were examined using a Self-Esteem Evaluation Scale, Empathic Fantasy Scale, and eight portions of the Imaginal Process Inventory reflecting eight types of fantasy use. For the patient group as a whole, a high degree of fantasy behavior was associated with low self-esteem, but the sex of the patient made a difference. Frequency of fantasy was shown to be indicative of poor adjustment for female dialysis patients, but not for male dialysis patients. Results also indicated that patients, without regard to sex, were more prone toward guilt fantasies in which they were punished for some wrongdoing such as feelings of anger toward people involved in their treatment or envy of others who do not suffer their same disability.", "contents": "Fantasy and self-esteem of renal dialysis patients and their spouses. The fantasy life and self-esteem of 22 male and 13 female renal dialysis patients and their spouses were examined using a Self-Esteem Evaluation Scale, Empathic Fantasy Scale, and eight portions of the Imaginal Process Inventory reflecting eight types of fantasy use. For the patient group as a whole, a high degree of fantasy behavior was associated with low self-esteem, but the sex of the patient made a difference. Frequency of fantasy was shown to be indicative of poor adjustment for female dialysis patients, but not for male dialysis patients. Results also indicated that patients, without regard to sex, were more prone toward guilt fantasies in which they were punished for some wrongdoing such as feelings of anger toward people involved in their treatment or envy of others who do not suffer their same disability.", "PMID": 982109}
{"id": "pubmed23n0032_12924", "title": "The cost of auditing outpatient records.", "content": "When patient records are to be auditied in a program designed to assess the quality of medical care rendered, careful consideration must be given to the cost of the system implemented. Structured data collection and a defined treatment plan are advantageous in facilitating the use of nonphysicians for the majority of the audit. A system using checklists to ensure adequate recording of subjective and objective data and a defined treatment plan for a common symptom complex were implemented by physicians and physician's assistants with a patient load averaging more than 1,000 patient contacts per week in a general medical clinic at Duke University Medical Center. Audit was subsequently accomplished at a cost of 96 cents per record. To reduce this cost, more efficient methods of selecting records for audit should be developed.", "contents": "The cost of auditing outpatient records. When patient records are to be auditied in a program designed to assess the quality of medical care rendered, careful consideration must be given to the cost of the system implemented. Structured data collection and a defined treatment plan are advantageous in facilitating the use of nonphysicians for the majority of the audit. A system using checklists to ensure adequate recording of subjective and objective data and a defined treatment plan for a common symptom complex were implemented by physicians and physician's assistants with a patient load averaging more than 1,000 patient contacts per week in a general medical clinic at Duke University Medical Center. Audit was subsequently accomplished at a cost of 96 cents per record. To reduce this cost, more efficient methods of selecting records for audit should be developed.", "PMID": 982110}
{"id": "pubmed23n0032_12925", "title": "Treatment of cystic hygroma.", "content": "To determine ideal treatment, 58 patients with cystic hygroma of the neck were studied. Approximately equal distribution of the lesion among sexes and sides of the neck was noted, but frequency was less than expected among blacks. Complete excision, the treatment of choice, gave an 81% cure rate. Partial excision was curative in only 12% of cases, but it decreased the tumor mass enough to eliminate the need for further treatment in 62% of cases. Aspiration of the cysts may be of value in diagnosis and management of acute respiratory problems but not for definitive therapy. Sclerosing agents and radiation therapy was not effective treatment of cystic hygromas. Of the clinical recurrences, 76% occurred within a year after therapy.", "contents": "Treatment of cystic hygroma. To determine ideal treatment, 58 patients with cystic hygroma of the neck were studied. Approximately equal distribution of the lesion among sexes and sides of the neck was noted, but frequency was less than expected among blacks. Complete excision, the treatment of choice, gave an 81% cure rate. Partial excision was curative in only 12% of cases, but it decreased the tumor mass enough to eliminate the need for further treatment in 62% of cases. Aspiration of the cysts may be of value in diagnosis and management of acute respiratory problems but not for definitive therapy. Sclerosing agents and radiation therapy was not effective treatment of cystic hygromas. Of the clinical recurrences, 76% occurred within a year after therapy.", "PMID": 982111}
{"id": "pubmed23n0032_12926", "title": "Association of tuberculosis with alcoholism.", "content": "To define the extent of the association between tuberculosis and alcoholism, all cases of tuberculosis reported in 1972 to the Fulton County Health Department (Georgia) were reviewed. Alcoholics accountered for 49% of all adult cases. Because of this high association, alcoholics should be screened regularly for possible tuberculosis.", "contents": "Association of tuberculosis with alcoholism. To define the extent of the association between tuberculosis and alcoholism, all cases of tuberculosis reported in 1972 to the Fulton County Health Department (Georgia) were reviewed. Alcoholics accountered for 49% of all adult cases. Because of this high association, alcoholics should be screened regularly for possible tuberculosis.", "PMID": 982112}
{"id": "pubmed23n0032_12927", "title": "Obscure distal femoral epiphyseal injury.", "content": "Undisplaced type I epiphyseal fracture of the distal femur in the adolescent athlete may mimic injury to a ligament. Roentgenographic examination with the knee under stress will reveal the diagnosis, thereby avoiding unnecessary arthrotomy and dictating appropriate treatment and follow-up.", "contents": "Obscure distal femoral epiphyseal injury. Undisplaced type I epiphyseal fracture of the distal femur in the adolescent athlete may mimic injury to a ligament. Roentgenographic examination with the knee under stress will reveal the diagnosis, thereby avoiding unnecessary arthrotomy and dictating appropriate treatment and follow-up.", "PMID": 982113}
{"id": "pubmed23n0032_12928", "title": "Vaginal candidiasis: its diagnosis and relation to urinary infection.", "content": "Of candidal vaginal cultures performed on 100 women who gave a history of a specific symptom complex, 85% were positive. In contrast, only 3% were positive in 100 women who did not have this history. The data indicate a definite relationship between the symptom complex and vaginal candidiasis. An additional 110 women had vaginal cultures on MacConkey's agar to determine the presence of gram-negative pathogens. The results suggest that a relationship may exist between the presence of vaginal candidiasis and an increased incidence of vaginal gram-negative organisms which could have important urologic significance.", "contents": "Vaginal candidiasis: its diagnosis and relation to urinary infection. Of candidal vaginal cultures performed on 100 women who gave a history of a specific symptom complex, 85% were positive. In contrast, only 3% were positive in 100 women who did not have this history. The data indicate a definite relationship between the symptom complex and vaginal candidiasis. An additional 110 women had vaginal cultures on MacConkey's agar to determine the presence of gram-negative pathogens. The results suggest that a relationship may exist between the presence of vaginal candidiasis and an increased incidence of vaginal gram-negative organisms which could have important urologic significance.", "PMID": 982114}
{"id": "pubmed23n0032_12929", "title": "A review of tibial lengthening procedures.", "content": "Eight patients had tibial lengthening procedures according to Anderson's method. The necessity of early determination of callus formation and bone grafting, if necessary, is emphasized. The potential morbidity in this procedure is great and the parents must be made fully aware of them. As Anderson states, it is a procedure that should be reserved for the child between 8 and 12 years old with a predictable discrepancy between 4 and 15 cm. We believe it is a useful procedure in problems of unequal length of the lower limbs but should not be used whem simpler procedures such as femoral shortening or epiphyseal arrest are indicated.", "contents": "A review of tibial lengthening procedures. Eight patients had tibial lengthening procedures according to Anderson's method. The necessity of early determination of callus formation and bone grafting, if necessary, is emphasized. The potential morbidity in this procedure is great and the parents must be made fully aware of them. As Anderson states, it is a procedure that should be reserved for the child between 8 and 12 years old with a predictable discrepancy between 4 and 15 cm. We believe it is a useful procedure in problems of unequal length of the lower limbs but should not be used whem simpler procedures such as femoral shortening or epiphyseal arrest are indicated.", "PMID": 982115}
{"id": "pubmed23n0032_12930", "title": "Agranulocytosis secondary to methimazole therapy: report of two cases.", "content": "Seventy-three cases of thyrotoxicosis were treated at Lloyd Noland Hospital with methimazole, propylthiouracil or both. Two cases of agranulocytosis occurred (2.7%) secondary to methimazole. Both responded to hospitalization, reverse isolation, and antibiotic coverage with complete recovery of the peripheral blood picture. The toxicity of methimazole is noted. The need for careful monitoring of blood counts during therapy and immediate discontinuance of the drug at the first clinical sign of granulocytopenia is stressed.", "contents": "Agranulocytosis secondary to methimazole therapy: report of two cases. Seventy-three cases of thyrotoxicosis were treated at Lloyd Noland Hospital with methimazole, propylthiouracil or both. Two cases of agranulocytosis occurred (2.7%) secondary to methimazole. Both responded to hospitalization, reverse isolation, and antibiotic coverage with complete recovery of the peripheral blood picture. The toxicity of methimazole is noted. The need for careful monitoring of blood counts during therapy and immediate discontinuance of the drug at the first clinical sign of granulocytopenia is stressed.", "PMID": 982116}
{"id": "pubmed23n0032_12931", "title": "Placental transport of nutrients.", "content": "The fetus is dependent upon the placenta for transport to it of all nutrients for energy and growth. The primary nutrients crossing the placenta are glucose, lactic acid, free amino acids, free fatty acids, and ketone bodies. Under normal circumstances, it appears that glucose is the primary energy source. In abnormal conditions there is the possibility that energy requirements may be met also by protein-amino acid oxidation and ketone body metabolism. The fetal brain may be quite adept in the use of ketone bodies. In all transport phenomena, the placenta intervenes by diverting nutrients into its own intermediary metabolism. For a more extensive consideration of these factors, several in-depth reviews are recommended. To better understand abnormalities of intrauterine growth, we need much more specific information about the mechanisms of placental transport and the intermediary metabolism of the placenta and fetus. When these are adequately in states of normal and abnormal intrauterine growth, we can then consider methods of metabolic intervention which will correct intrauterine growth failure and, hopefully, eliminate its hazards to the fetus and child.", "contents": "Placental transport of nutrients. The fetus is dependent upon the placenta for transport to it of all nutrients for energy and growth. The primary nutrients crossing the placenta are glucose, lactic acid, free amino acids, free fatty acids, and ketone bodies. Under normal circumstances, it appears that glucose is the primary energy source. In abnormal conditions there is the possibility that energy requirements may be met also by protein-amino acid oxidation and ketone body metabolism. The fetal brain may be quite adept in the use of ketone bodies. In all transport phenomena, the placenta intervenes by diverting nutrients into its own intermediary metabolism. For a more extensive consideration of these factors, several in-depth reviews are recommended. To better understand abnormalities of intrauterine growth, we need much more specific information about the mechanisms of placental transport and the intermediary metabolism of the placenta and fetus. When these are adequately in states of normal and abnormal intrauterine growth, we can then consider methods of metabolic intervention which will correct intrauterine growth failure and, hopefully, eliminate its hazards to the fetus and child.", "PMID": 982117}
{"id": "pubmed23n0032_12932", "title": "A comparison of modern methods of reduction mammaplasty.", "content": "There are many technics available today for reducing the oversize female breast. The most commonly used operations may be divided into two catergories, depending on treatment of the nipple-areolar complex: (1) free graft or (2) transposition on underlying tissue. According to this division, various methods are outlined and discussed.", "contents": "A comparison of modern methods of reduction mammaplasty. There are many technics available today for reducing the oversize female breast. The most commonly used operations may be divided into two catergories, depending on treatment of the nipple-areolar complex: (1) free graft or (2) transposition on underlying tissue. According to this division, various methods are outlined and discussed.", "PMID": 982118}
{"id": "pubmed23n0032_12933", "title": "Polymyositis: response to vitamin E.", "content": "A 70-year-old woman with polymyositis was treated with three different immunosuppressive drugs. Her condition deteriorated over a three-month period until she became totally helpness. She then made a dramatic improvement when large doses of vitamin E (d, alpha-tocopheryl acetate) were administered. Current knowledge regarding the nature of polymyositis and the rationale for using vitamin E to treat it are discussed.", "contents": "Polymyositis: response to vitamin E. A 70-year-old woman with polymyositis was treated with three different immunosuppressive drugs. Her condition deteriorated over a three-month period until she became totally helpness. She then made a dramatic improvement when large doses of vitamin E (d, alpha-tocopheryl acetate) were administered. Current knowledge regarding the nature of polymyositis and the rationale for using vitamin E to treat it are discussed.", "PMID": 982119}
{"id": "pubmed23n0032_12934", "title": "The localization of multiple intra-abdominal abscesses by gallium scanning.", "content": "A septic course suggestive of intra-abdominal abscess followed surgery in a small child with a perforated appendix. Usual diagnostic studies, fluoroscopy, and rectal and wound exploration failed to localized an abscess. The use of gallium 67 scanning accurately localized multiple abscesses which were successfully drained.", "contents": "The localization of multiple intra-abdominal abscesses by gallium scanning. A septic course suggestive of intra-abdominal abscess followed surgery in a small child with a perforated appendix. Usual diagnostic studies, fluoroscopy, and rectal and wound exploration failed to localized an abscess. The use of gallium 67 scanning accurately localized multiple abscesses which were successfully drained.", "PMID": 982120}
{"id": "pubmed23n0032_12935", "title": "Isolation procedures for thyroglobulin: effects of phenylmethanesulfonyl fluoride and freezing.", "content": "The presence of fast-migrating, low-molecular weight components in normal rat thyroglobulin, iodine-poor rat thyroglobulin and normal bovine thyroglobulin was investigated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. When normal and iodine-poor rat thyroglobulin were extracted in the presence of phenylmethanesulfonyl fluoride, a serine protease inhibitor, very few components migrating faster than the 12S half-molecule were found. In normal bovine thyroglobulin no effect of the protease inhibitor on the formation of fast-moving components was found; however, prior freezing of the glands greatly influenced the presence of these components. Thyroglobulin obtained from bovine glands without any prior freezing, contained no noncovalently-bound band migrating faster than 12S.", "contents": "Isolation procedures for thyroglobulin: effects of phenylmethanesulfonyl fluoride and freezing. The presence of fast-migrating, low-molecular weight components in normal rat thyroglobulin, iodine-poor rat thyroglobulin and normal bovine thyroglobulin was investigated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. When normal and iodine-poor rat thyroglobulin were extracted in the presence of phenylmethanesulfonyl fluoride, a serine protease inhibitor, very few components migrating faster than the 12S half-molecule were found. In normal bovine thyroglobulin no effect of the protease inhibitor on the formation of fast-moving components was found; however, prior freezing of the glands greatly influenced the presence of these components. Thyroglobulin obtained from bovine glands without any prior freezing, contained no noncovalently-bound band migrating faster than 12S.", "PMID": 982189}
{"id": "pubmed23n0032_12936", "title": "Monitoring aminopentamide urinary excretion by means of multiple 'microphase' extraction - a rapid method for the extraction and concentration of small amounts of lipophilic drugs from large volumes of biological fluids without distillation.", "content": "It has been demonstrated that low concentrations of basic lipophilic drugs in biological fluids may be extracted and concentrated 10(4)-10(6) times by a series of extraction procedures in which the ratio of the extracting solvent to that of the solution to be extracted is of the order of 1:100 (microphase extraction procedure). Typically, basic drugs (atropine, aminopentamide, hyoscine, chloroquine, pyrimethamine) were extracted and concentrated sufficiently for direct GC analysis from 24-hour urine samples by a procedure involving three simple consecutive extraction steps. Using this procedure, it was demonstrated that after administration of aminopentamide (300 micrograms) to patients in the form of anti-diarrhoeal tablets, measurable quantities of the free, unchanged drug can be demonstrated in 24-hour urine samples. The main advantages of the method are simplicity, rapidity and sensitivity due to the low background interference in the GC separations. The principle involved can be extended to the analysis of acidic drugs with suitable solubility properties.", "contents": "Monitoring aminopentamide urinary excretion by means of multiple 'microphase' extraction - a rapid method for the extraction and concentration of small amounts of lipophilic drugs from large volumes of biological fluids without distillation. It has been demonstrated that low concentrations of basic lipophilic drugs in biological fluids may be extracted and concentrated 10(4)-10(6) times by a series of extraction procedures in which the ratio of the extracting solvent to that of the solution to be extracted is of the order of 1:100 (microphase extraction procedure). Typically, basic drugs (atropine, aminopentamide, hyoscine, chloroquine, pyrimethamine) were extracted and concentrated sufficiently for direct GC analysis from 24-hour urine samples by a procedure involving three simple consecutive extraction steps. Using this procedure, it was demonstrated that after administration of aminopentamide (300 micrograms) to patients in the form of anti-diarrhoeal tablets, measurable quantities of the free, unchanged drug can be demonstrated in 24-hour urine samples. The main advantages of the method are simplicity, rapidity and sensitivity due to the low background interference in the GC separations. The principle involved can be extended to the analysis of acidic drugs with suitable solubility properties.", "PMID": 982190}
{"id": "pubmed23n0032_12937", "title": "Inhibition of the uptake of [13h] thymidine into hamster embryo cells by cytosine arabinoside.", "content": "1-beta-D Arabinofuranosylcytosine (ara-C) has been shown to strongly inhibit the uptake of [13h] thymidine into hamster embryo fibroblasts and transformed hamster embryo cells.", "contents": "Inhibition of the uptake of [13h] thymidine into hamster embryo cells by cytosine arabinoside. 1-beta-D Arabinofuranosylcytosine (ara-C) has been shown to strongly inhibit the uptake of [13h] thymidine into hamster embryo fibroblasts and transformed hamster embryo cells.", "PMID": 982191}
{"id": "pubmed23n0032_12938", "title": "An experimental study on the onset of endolymphatic secretion in chick embryos.", "content": "Extirpation of the endolymphatic anlage of the ear vesicle or that part of the ear placode which develops into the endolymphatic anlage in chick embryos, resulted in the absence of the endolymphatic system. In specimens of which the endolymphatic anlage was removed on the fourth day, healing of the wound was completed soon after the fifth day of incubation. Most of these experimental animals developed endolymphatic hydrops at a later stage. However, where healing was delayed after the fifth day, the open wound acted as an escape route for the endolymph (thus preventing further closure) which at that stage (or soon after) reached an appreciable volume.", "contents": "An experimental study on the onset of endolymphatic secretion in chick embryos. Extirpation of the endolymphatic anlage of the ear vesicle or that part of the ear placode which develops into the endolymphatic anlage in chick embryos, resulted in the absence of the endolymphatic system. In specimens of which the endolymphatic anlage was removed on the fourth day, healing of the wound was completed soon after the fifth day of incubation. Most of these experimental animals developed endolymphatic hydrops at a later stage. However, where healing was delayed after the fifth day, the open wound acted as an escape route for the endolymph (thus preventing further closure) which at that stage (or soon after) reached an appreciable volume.", "PMID": 982192}
{"id": "pubmed23n0032_12939", "title": "Endoscopic retrograde cholangiopancreatography in the evaluation of pancreatic disease.", "content": "Endoscopic retrograde cholangiopancreatography (ERCP) was carried out in 98 patients with unexplained abdominal pain or known pancreatitis with recurrent pain. Patients with jaundice were excluded from the study. In 38 patients with a clinical diagnosis of pancreatitis, the radiological findings on ERCP were graded according to the criteria of Kasugai et al. Advanced pancreatitis was found in 20 patients (52,5%), moderate changes in 7 (18,4%) and minimal-change pancreatitis in 6 (15,8%). ERCP had normal pancreatic function tests. In 35 patients investigated for unexplained abdominal pain, changes consistent with pancreatitis were found in 7, pancreatic carcinoma in 5, a duodenal ulcer in 2, gallstones in 1 and a duodenal tumour in 1. ERCP was normal in 19 patients. A comparison of the findings on ERCP and the standard secretin-cholecystokinin pancreatic function test was available in 52 patients. There was a good agreement between the two tests in the patients with advanced or moderate pancreatitis as revealed by ERCP, but less agreement in the patients with minimal-change pancreatitis. A few patients with clinical pancreatitis and abnormal ERCP had normal pancreatic function tests. ERCP increases the diagnostic yield in patients suspected of having pancreatitis and is at present the only reliable method of diagnosing pancreatic carcinoma which is not evident by other non-operative techniques. ERCP is also of value in the assessment of the severity of pancreatitis and is a necessary investigation before pancreatic surgery to confirm or exclude cyst formation or the site of duct obstruction. The finding of an unsuspected cyst at ERCP necessitates early operation because of the danger of introducing infection during the procedure.", "contents": "Endoscopic retrograde cholangiopancreatography in the evaluation of pancreatic disease. Endoscopic retrograde cholangiopancreatography (ERCP) was carried out in 98 patients with unexplained abdominal pain or known pancreatitis with recurrent pain. Patients with jaundice were excluded from the study. In 38 patients with a clinical diagnosis of pancreatitis, the radiological findings on ERCP were graded according to the criteria of Kasugai et al. Advanced pancreatitis was found in 20 patients (52,5%), moderate changes in 7 (18,4%) and minimal-change pancreatitis in 6 (15,8%). ERCP had normal pancreatic function tests. In 35 patients investigated for unexplained abdominal pain, changes consistent with pancreatitis were found in 7, pancreatic carcinoma in 5, a duodenal ulcer in 2, gallstones in 1 and a duodenal tumour in 1. ERCP was normal in 19 patients. A comparison of the findings on ERCP and the standard secretin-cholecystokinin pancreatic function test was available in 52 patients. There was a good agreement between the two tests in the patients with advanced or moderate pancreatitis as revealed by ERCP, but less agreement in the patients with minimal-change pancreatitis. A few patients with clinical pancreatitis and abnormal ERCP had normal pancreatic function tests. ERCP increases the diagnostic yield in patients suspected of having pancreatitis and is at present the only reliable method of diagnosing pancreatic carcinoma which is not evident by other non-operative techniques. ERCP is also of value in the assessment of the severity of pancreatitis and is a necessary investigation before pancreatic surgery to confirm or exclude cyst formation or the site of duct obstruction. The finding of an unsuspected cyst at ERCP necessitates early operation because of the danger of introducing infection during the procedure.", "PMID": 982198}
{"id": "pubmed23n0032_12940", "title": "[Ebstein's anomaly of the tricuspid valve: a case report].", "content": "A case of Ebstein's malformation of the tricuspid valve with a complicating ventricular septal defect in a 3-year-old Black child is presented. A pre-operative diagnosis of an atrioventricular canal was made, and only at operation was it discovered to be an Ebstein's anomaly of the tricuspid valve. The clinical picture and diagnoses are reviewed, and the problem of a correct pre-operative diagnosis is discussed. The operative treatment and postoperative result in our patient is described.", "contents": "[Ebstein's anomaly of the tricuspid valve: a case report]. A case of Ebstein's malformation of the tricuspid valve with a complicating ventricular septal defect in a 3-year-old Black child is presented. A pre-operative diagnosis of an atrioventricular canal was made, and only at operation was it discovered to be an Ebstein's anomaly of the tricuspid valve. The clinical picture and diagnoses are reviewed, and the problem of a correct pre-operative diagnosis is discussed. The operative treatment and postoperative result in our patient is described.", "PMID": 982199}
{"id": "pubmed23n0032_12941", "title": "Biological assessment of protein quality. Optimal essential: non-essential amino acid ratios for maintenance of certain states of nitrogen balance in young rats.", "content": "Male weanling rats were used to assess the effect of replacing essential amino acid (EAA) mixtures with non-essential amino acid (NEAA) mixtures on nitrogen balance. Each of 4 essential amino acid mixtures, fed in quantities sufficient to maintain specific states of nitrogen balance, was replaced with a non-essential amino acid mixture on an isonitrogenous basis. The results indicate the following: 1. Within limits, replacement with NEAA mixture caused an increase in the efficiency of utilisation of the amino acid mixture when the nitrogen balance state investigated was + 100, +200 or +300 mg/100 g body mass/8 days, but not when nitrogen balance was zero. 2. In the maintenance of nitrogen balance = 0, the EAA mixture could be replaced with NEAA mixture to a greater extent (83%) than was possible in the maintenance of balance states of + 100, +200 or +300 mg (about 70%). The results support the view that (a) EAAs are converted to NEAAs at the cost of metabolically available nitrogen: and (b) with a decrease in the state of nitrogen balance maintained by the animal, the amino acid requirement is shifted partially from EAAs to NEAAs. The implications of the above findings are discussed with reference to dietary protein evaluation and related questions.", "contents": "Biological assessment of protein quality. Optimal essential: non-essential amino acid ratios for maintenance of certain states of nitrogen balance in young rats. Male weanling rats were used to assess the effect of replacing essential amino acid (EAA) mixtures with non-essential amino acid (NEAA) mixtures on nitrogen balance. Each of 4 essential amino acid mixtures, fed in quantities sufficient to maintain specific states of nitrogen balance, was replaced with a non-essential amino acid mixture on an isonitrogenous basis. The results indicate the following: 1. Within limits, replacement with NEAA mixture caused an increase in the efficiency of utilisation of the amino acid mixture when the nitrogen balance state investigated was + 100, +200 or +300 mg/100 g body mass/8 days, but not when nitrogen balance was zero. 2. In the maintenance of nitrogen balance = 0, the EAA mixture could be replaced with NEAA mixture to a greater extent (83%) than was possible in the maintenance of balance states of + 100, +200 or +300 mg (about 70%). The results support the view that (a) EAAs are converted to NEAAs at the cost of metabolically available nitrogen: and (b) with a decrease in the state of nitrogen balance maintained by the animal, the amino acid requirement is shifted partially from EAAs to NEAAs. The implications of the above findings are discussed with reference to dietary protein evaluation and related questions.", "PMID": 982200}
{"id": "pubmed23n0032_12942", "title": "Delayed onset of respiratory obstruction during endotracheal anaesthesia.", "content": "Two patients in whom respiratory obstruction with an insidious onset occurred during the use of a Lapro endotracheal tube are described. In one patient, the valvular nature of the obstruction produced bilateral tension pneumothoraces.", "contents": "Delayed onset of respiratory obstruction during endotracheal anaesthesia. Two patients in whom respiratory obstruction with an insidious onset occurred during the use of a Lapro endotracheal tube are described. In one patient, the valvular nature of the obstruction produced bilateral tension pneumothoraces.", "PMID": 982207}
{"id": "pubmed23n0032_12943", "title": "The prenatal diagnosis of Tay-Sachs disease.", "content": "Tay-Sachs disease was diagnosed prenatally on the basis of enzyme assays and the electrophoretic pattern of extracts made from cultured amniotic fluid cells. Electrophoresis revealed the presence of hexosaminidase B but no hexosaminidase A, the iso-enzyme which is deficient in Tay-Sachs disease. Estimations of the percentage of hexosaminidase A present in cultured amniotic cell extracts by means of thermal fractionation revealed the presence of only small amounts of this isozyme. Results obtained on amniotic fluid alone were found to be unreliable and were disregarded when making the diagnosis. Following termination of the pregnancy, the diagnosis was confirmed by electrophoresis and the thermal inactivation method as well as by electron microscopy. A slight lower temperature for the thermal inactivation has been found to give better discrimination than the temperature used in previously described techniques.", "contents": "The prenatal diagnosis of Tay-Sachs disease. Tay-Sachs disease was diagnosed prenatally on the basis of enzyme assays and the electrophoretic pattern of extracts made from cultured amniotic fluid cells. Electrophoresis revealed the presence of hexosaminidase B but no hexosaminidase A, the iso-enzyme which is deficient in Tay-Sachs disease. Estimations of the percentage of hexosaminidase A present in cultured amniotic cell extracts by means of thermal fractionation revealed the presence of only small amounts of this isozyme. Results obtained on amniotic fluid alone were found to be unreliable and were disregarded when making the diagnosis. Following termination of the pregnancy, the diagnosis was confirmed by electrophoresis and the thermal inactivation method as well as by electron microscopy. A slight lower temperature for the thermal inactivation has been found to give better discrimination than the temperature used in previously described techniques.", "PMID": 982208}
{"id": "pubmed23n0032_12944", "title": "The use of transfer factors in the treatment of multiple sclerosis: a case report.", "content": "A 23-year-old woman suffering from multiple sclerosis (MS) was given a course of 6 injections (0.5 U) of mumps transfer factor (TF) at 2-weekly intervals. The patient's symptoms improved dramatically and during the 10 months since treatment was instituted, no exacerbations have occurred. The possible aetiological role of paramyxoviruses in MS and the use of TF in the treatment of the disease are discussed. MS is however, a disease of exacerbations and spontaneous remissions, and despite our promising findings in this patient we wish to refrain from hailing mumps TF as a possible cure for this notorious disease.", "contents": "The use of transfer factors in the treatment of multiple sclerosis: a case report. A 23-year-old woman suffering from multiple sclerosis (MS) was given a course of 6 injections (0.5 U) of mumps transfer factor (TF) at 2-weekly intervals. The patient's symptoms improved dramatically and during the 10 months since treatment was instituted, no exacerbations have occurred. The possible aetiological role of paramyxoviruses in MS and the use of TF in the treatment of the disease are discussed. MS is however, a disease of exacerbations and spontaneous remissions, and despite our promising findings in this patient we wish to refrain from hailing mumps TF as a possible cure for this notorious disease.", "PMID": 982209}
{"id": "pubmed23n0032_12945", "title": "Amylase: creatinine clearance ratio and urinary excretion of lysozyme in acute pancreatitis and acute duodenal perforation.", "content": "The amylase:creatinine clearance ratio in patients suffering from acute pancreatitis or acute duodenal perforation was higher than normal in both groups of patients. These findings cast doubt on the value of this parameter as a specific index of acute pancreatitis. The mechanism or mechanisms underlying the increased amylase excretion have not been determined. However, the markedly elevated urinary excretion of lysozyme observed in some patients suggests, by analogy, that diminished tubular reabsorption of amylase may contribute towards the elevated amylase:creatinine ratio.", "contents": "Amylase: creatinine clearance ratio and urinary excretion of lysozyme in acute pancreatitis and acute duodenal perforation. The amylase:creatinine clearance ratio in patients suffering from acute pancreatitis or acute duodenal perforation was higher than normal in both groups of patients. These findings cast doubt on the value of this parameter as a specific index of acute pancreatitis. The mechanism or mechanisms underlying the increased amylase excretion have not been determined. However, the markedly elevated urinary excretion of lysozyme observed in some patients suggests, by analogy, that diminished tubular reabsorption of amylase may contribute towards the elevated amylase:creatinine ratio.", "PMID": 982210}
{"id": "pubmed23n0032_12946", "title": "Biochemical parameters in athletes before and after having run 160 kilometres.", "content": "A number of biochemical and haematological parameters, including plasma electrolytes, parameters of hepatic and renal function, plasma enzymes and free fatty acids were measured in 13 athletes before and after a 160-km 24-hour race. The runners were divided into 2 groups: group A, who competed the 160 km within 24 hours and group B, who either ran for 24 hours, or who retired before completing the distance. Minimal changes were found in the plasma electrolyte patterns in either group, whereas blood urea and creatinine levels increased during the race. The plasma enzymes increased to varying extents, the greatest increases being in lactic dehydrogenase, aspartate aminotransferase and the skeletal muscle specific MM isoenzyme of creatinine phosphokinase. Total bilirubin also increased, but no conclusive evidence of hepatic decompensation was found. Plasma free fatty acids levels were very markedly raised in 12 of the runners, the highest increases occurring in group A. All runners ingested carbohydrate during the race and this probably explains why the blood glucose levels increased slightly but remained within normal limits in all the athletes at the end of the race.", "contents": "Biochemical parameters in athletes before and after having run 160 kilometres. A number of biochemical and haematological parameters, including plasma electrolytes, parameters of hepatic and renal function, plasma enzymes and free fatty acids were measured in 13 athletes before and after a 160-km 24-hour race. The runners were divided into 2 groups: group A, who competed the 160 km within 24 hours and group B, who either ran for 24 hours, or who retired before completing the distance. Minimal changes were found in the plasma electrolyte patterns in either group, whereas blood urea and creatinine levels increased during the race. The plasma enzymes increased to varying extents, the greatest increases being in lactic dehydrogenase, aspartate aminotransferase and the skeletal muscle specific MM isoenzyme of creatinine phosphokinase. Total bilirubin also increased, but no conclusive evidence of hepatic decompensation was found. Plasma free fatty acids levels were very markedly raised in 12 of the runners, the highest increases occurring in group A. All runners ingested carbohydrate during the race and this probably explains why the blood glucose levels increased slightly but remained within normal limits in all the athletes at the end of the race.", "PMID": 982211}
{"id": "pubmed23n0032_12947", "title": "Differential effect of right and left vagal stimulation on right and left circumflex coronary arteries.", "content": "In anasthetised, closed-chest dogs in complete heart block that were paced at a ventricular rate of 100 beats/min, vagal stimulation increased right coronary (RC) flow by 46% and conductance by 59%, but these increases were less than those measured in the left circumflex coronary (LCC) flow (66%) and conductance (80%). Both the right and left vagus nerves affected RC and LCC flow, the left vagus having the greater effect. The response to vagal stimulation is not limited at higher ventricular rates.", "contents": "Differential effect of right and left vagal stimulation on right and left circumflex coronary arteries. In anasthetised, closed-chest dogs in complete heart block that were paced at a ventricular rate of 100 beats/min, vagal stimulation increased right coronary (RC) flow by 46% and conductance by 59%, but these increases were less than those measured in the left circumflex coronary (LCC) flow (66%) and conductance (80%). Both the right and left vagus nerves affected RC and LCC flow, the left vagus having the greater effect. The response to vagal stimulation is not limited at higher ventricular rates.", "PMID": 982212}
{"id": "pubmed23n0032_12948", "title": "Constrictive pericarditis due to methysergide therapy.", "content": "A 39-year-old woman who suffered from migraine is described. She developed analgesic nephropathy due to phenacetin, and constrictive pericarditis, probably due to methysergide treatment.", "contents": "Constrictive pericarditis due to methysergide therapy. A 39-year-old woman who suffered from migraine is described. She developed analgesic nephropathy due to phenacetin, and constrictive pericarditis, probably due to methysergide treatment.", "PMID": 982213}
{"id": "pubmed23n0032_12949", "title": "Modification of mild proteinuria by postural and other mechanisms affecting haemodynamics.", "content": "Changes in urinary protein excretion induced by standing or by the application of venous tourniquets to the thighs while the patient is in the supine position were studied in patients with mild proteinuria and compared with the changes that occur in severe proteinuria (greater than 1 mg/min). Protein excretion decreased in the majority of patients. Irrespective of the initial degree of proteinuria. The increased rate of protein excretion that occurred in a minority of patients when standing may represent a phenomenon analogous to orthostatic proteinuria.", "contents": "Modification of mild proteinuria by postural and other mechanisms affecting haemodynamics. Changes in urinary protein excretion induced by standing or by the application of venous tourniquets to the thighs while the patient is in the supine position were studied in patients with mild proteinuria and compared with the changes that occur in severe proteinuria (greater than 1 mg/min). Protein excretion decreased in the majority of patients. Irrespective of the initial degree of proteinuria. The increased rate of protein excretion that occurred in a minority of patients when standing may represent a phenomenon analogous to orthostatic proteinuria.", "PMID": 982214}
{"id": "pubmed23n0032_12950", "title": "Severe measles-associated pneumonia treated with assisted ventilation.", "content": "Measles-associated pneumonia, which was severe enough to require mechanical ventilation, caused a mortality of 64%. The main indications for special respiratory care were severe infection and hypoxaemia. Complications of the disease occurred in 78% of the patients. The commonest were anaemia, enteritis and cardiac failure, and they contributed to the grave prognosis. Viral pneumonia was present in most of the patients who died; superinfection was rare. The characteristics of measles virus was present in 30% and of adenovirus in no less that 40%.", "contents": "Severe measles-associated pneumonia treated with assisted ventilation. Measles-associated pneumonia, which was severe enough to require mechanical ventilation, caused a mortality of 64%. The main indications for special respiratory care were severe infection and hypoxaemia. Complications of the disease occurred in 78% of the patients. The commonest were anaemia, enteritis and cardiac failure, and they contributed to the grave prognosis. Viral pneumonia was present in most of the patients who died; superinfection was rare. The characteristics of measles virus was present in 30% and of adenovirus in no less that 40%.", "PMID": 982215}
{"id": "pubmed23n0032_12951", "title": "Cryptococcal meningitis: our experience in 24 black patients.", "content": "The records of 24 patients with cryptococcal meningitis, admitted to King Edward VIII Hospital in Durban, were reviewed. There were 21 adults and 3 children over periods of 10 and 18 years, respectively. Slightly more males were affected. The age distribution was fairly even. The common clinical presentations were headache, neck stiffness, mental changes, cranial nerve palsies and papilloedema. The diagnosis was proved by the presense of Cryptococcus neoformans in the cerebrospinal fluid (CSF) in 21 of the patients, and in pathological tissue in the remaining 3 patients. A significant percentage of patients presented with an initial polymorphonuclear leucocyte predominance in the CSF. The other CSF findings were elevated protein and low sugar levels. Tuberculosis was the common coexisting disease. We found the mortality rate of 58% to be very high and a significant number of patients died even after adequate treatment with amphotericin B either singly or in combination with 5-fluorocytosine.", "contents": "Cryptococcal meningitis: our experience in 24 black patients. The records of 24 patients with cryptococcal meningitis, admitted to King Edward VIII Hospital in Durban, were reviewed. There were 21 adults and 3 children over periods of 10 and 18 years, respectively. Slightly more males were affected. The age distribution was fairly even. The common clinical presentations were headache, neck stiffness, mental changes, cranial nerve palsies and papilloedema. The diagnosis was proved by the presense of Cryptococcus neoformans in the cerebrospinal fluid (CSF) in 21 of the patients, and in pathological tissue in the remaining 3 patients. A significant percentage of patients presented with an initial polymorphonuclear leucocyte predominance in the CSF. The other CSF findings were elevated protein and low sugar levels. Tuberculosis was the common coexisting disease. We found the mortality rate of 58% to be very high and a significant number of patients died even after adequate treatment with amphotericin B either singly or in combination with 5-fluorocytosine.", "PMID": 982216}
{"id": "pubmed23n0032_12952", "title": "Schistosomiasis and carcinoma of the bladder in Zambia.", "content": "Carcinoma of the bladder is the third most important malignancy seen in Zambia, accounting for nearly 9% of all malignancies seen in the Department of Pathology at the University of Zambia. A review of 217 cases of carcinoma of the bladder seen in the department over a 5-year period revealed a preponderance of well-differentiated squamous cell carcinoma (75%). Of the 217 cases, 65% had concomitant schistosomiasis. These tumours tended to occur in a younger age group than that reported from England and North America. The pattern of pathology conforms with that reported from other parts of Africa with hyperendemic and endemic schistosomiasis. The aetiological role of schistosomiasis in bladder cancer is critically examined.", "contents": "Schistosomiasis and carcinoma of the bladder in Zambia. Carcinoma of the bladder is the third most important malignancy seen in Zambia, accounting for nearly 9% of all malignancies seen in the Department of Pathology at the University of Zambia. A review of 217 cases of carcinoma of the bladder seen in the department over a 5-year period revealed a preponderance of well-differentiated squamous cell carcinoma (75%). Of the 217 cases, 65% had concomitant schistosomiasis. These tumours tended to occur in a younger age group than that reported from England and North America. The pattern of pathology conforms with that reported from other parts of Africa with hyperendemic and endemic schistosomiasis. The aetiological role of schistosomiasis in bladder cancer is critically examined.", "PMID": 982217}
{"id": "pubmed23n0032_12953", "title": "Treatment of the ruptured uterus.", "content": "Three hundred and thirty-five patients with ruptured uterus were reviewed. The incidence of this condition is increasing, probably owing to poor antenatal care and lack of facilities. The most common site of rupture in the previously unscarred uterus was found to be longitudinal on the lateral aspects of the lower and upper segments. When the ruptures were in these areas, total hysterectomy proved to be best. Repair of the rupture should only be considered when the tear is simple, transverse in the lower segment, and in the absence of infection.", "contents": "Treatment of the ruptured uterus. Three hundred and thirty-five patients with ruptured uterus were reviewed. The incidence of this condition is increasing, probably owing to poor antenatal care and lack of facilities. The most common site of rupture in the previously unscarred uterus was found to be longitudinal on the lateral aspects of the lower and upper segments. When the ruptures were in these areas, total hysterectomy proved to be best. Repair of the rupture should only be considered when the tear is simple, transverse in the lower segment, and in the absence of infection.", "PMID": 982218}
{"id": "pubmed23n0032_12954", "title": "Oxygen transport during extracorporeal oxygenation for the treatment of adult respiratory distress syndrome.", "content": "An inventory of hemodynamic and respiratory measurements was obtained in two patients with acute respiratory failure syndrome prior to and during venoarterial extracorporeal membrane oxygenation for study of oxygen transport. As the inspired oxygen concentration fraction was reduced from 1.0 to 0.5 and paO2 was increased from less than 50 to physiologic ranges during extracorporeal membrane oxygenation, tachycardia and pulmonary hypertension were reversed. Total oxygen transport was reduced rather than increased. Oxygen consumption and oxygen extraction were not altered during extracorporeal membrane oxygenation. However, a striking reduction was observed in cardiac output. After reversal of anoxemia, the total of the cardiac output of the patient and the extracorporeal membrane oxygenation flow were less than the cardiac output of the patient prior to extracorporeal membrane oxygenation. These observations provide evidence that the beneficial effects of extracorporeal membrane oxygenation stem, at least in part, from a reduction of the work load on the heart. Since high cardiac output failure is observed during progression of adult respiratory distress syndrome, mechanical support of circulation during extracorporeal membrane oxygenation may be of primary therapeutic benefit.", "contents": "Oxygen transport during extracorporeal oxygenation for the treatment of adult respiratory distress syndrome. An inventory of hemodynamic and respiratory measurements was obtained in two patients with acute respiratory failure syndrome prior to and during venoarterial extracorporeal membrane oxygenation for study of oxygen transport. As the inspired oxygen concentration fraction was reduced from 1.0 to 0.5 and paO2 was increased from less than 50 to physiologic ranges during extracorporeal membrane oxygenation, tachycardia and pulmonary hypertension were reversed. Total oxygen transport was reduced rather than increased. Oxygen consumption and oxygen extraction were not altered during extracorporeal membrane oxygenation. However, a striking reduction was observed in cardiac output. After reversal of anoxemia, the total of the cardiac output of the patient and the extracorporeal membrane oxygenation flow were less than the cardiac output of the patient prior to extracorporeal membrane oxygenation. These observations provide evidence that the beneficial effects of extracorporeal membrane oxygenation stem, at least in part, from a reduction of the work load on the heart. Since high cardiac output failure is observed during progression of adult respiratory distress syndrome, mechanical support of circulation during extracorporeal membrane oxygenation may be of primary therapeutic benefit.", "PMID": 982247}
{"id": "pubmed23n0032_12955", "title": "Primary malignant tumors of the small intestine.", "content": "Primary malignant tumors of the small intestine are uncommon. This infrequency and possible lack of awareness can result in a late diagnosis and a poor survival time. In a period of 34 years, only 55 patients were seen at our cancer institute. The average age of the patients was 56 years, with a male predominance ratio of 2.6:1.0. Twenty-one patients had adenocarcinomas, 19 had sarcomas and 15 had carcinoids. The most common signs and symptoms were abdominal pain and obstruction of the intestine. Preoperative diagnosis was established in 12 of these patients only by roentgenologic barium examination of the small intestine. At the time of diagnosis, 34 of the patients had metastasis to regional lymph nodes or distant organs. The median and five year survival times were one year and 19 per cent, respectively. Patients with carcinoids had better survival rates than those with adenocarcinomas or sarcomas. Palliative resection did not improve survival time. However, if other therapeutic modalities also were used, it might prove beneficial. Patients with palpable abdominal masses or intestinal bleeding, or both, had a worse prognosis than did those presenting with obstruction of the intestine because these are late presenting symptoms. Therefore, recurrent abdominal pain should increase clinical suspicion, and early diagnosis by careful examination of the small intestine with barium contrast material could improve the survival time. Finally, it seemed that these tumors had a high incidence of coexisting malignant conditions, as nine of the patients in our series had a second malignant tumor.", "contents": "Primary malignant tumors of the small intestine. Primary malignant tumors of the small intestine are uncommon. This infrequency and possible lack of awareness can result in a late diagnosis and a poor survival time. In a period of 34 years, only 55 patients were seen at our cancer institute. The average age of the patients was 56 years, with a male predominance ratio of 2.6:1.0. Twenty-one patients had adenocarcinomas, 19 had sarcomas and 15 had carcinoids. The most common signs and symptoms were abdominal pain and obstruction of the intestine. Preoperative diagnosis was established in 12 of these patients only by roentgenologic barium examination of the small intestine. At the time of diagnosis, 34 of the patients had metastasis to regional lymph nodes or distant organs. The median and five year survival times were one year and 19 per cent, respectively. Patients with carcinoids had better survival rates than those with adenocarcinomas or sarcomas. Palliative resection did not improve survival time. However, if other therapeutic modalities also were used, it might prove beneficial. Patients with palpable abdominal masses or intestinal bleeding, or both, had a worse prognosis than did those presenting with obstruction of the intestine because these are late presenting symptoms. Therefore, recurrent abdominal pain should increase clinical suspicion, and early diagnosis by careful examination of the small intestine with barium contrast material could improve the survival time. Finally, it seemed that these tumors had a high incidence of coexisting malignant conditions, as nine of the patients in our series had a second malignant tumor.", "PMID": 982248}
{"id": "pubmed23n0032_12956", "title": "Impaired glucose flow in burned patients with gram-negative sepsis.", "content": "Fifteen thermally injured patients with positive blood stream cultures for gram-negative organisms demonstrated a decreased mass flow of glucose through the glucose space when compared with 17 patients without sepsis studied at a comparable time after injury. Amino acid concentrations determined in ten burned patients with sepsis and nine burned patients without sepsis revealed an increase in the gluconeogenic precursors alanine, glycine, methionine and phenylalanine in those patients with sepsis. The administration of alanine consistently increased serum glucose in seven patients without sepsis but exerted no effect on glucose concentrations in six person with sepsis. These data, taken together, indicate that gram-negative sepsis in burned patients impairs the increased rate of glucose production and flow to peripheral tissue which characteristically occurs after thermal injury.", "contents": "Impaired glucose flow in burned patients with gram-negative sepsis. Fifteen thermally injured patients with positive blood stream cultures for gram-negative organisms demonstrated a decreased mass flow of glucose through the glucose space when compared with 17 patients without sepsis studied at a comparable time after injury. Amino acid concentrations determined in ten burned patients with sepsis and nine burned patients without sepsis revealed an increase in the gluconeogenic precursors alanine, glycine, methionine and phenylalanine in those patients with sepsis. The administration of alanine consistently increased serum glucose in seven patients without sepsis but exerted no effect on glucose concentrations in six person with sepsis. These data, taken together, indicate that gram-negative sepsis in burned patients impairs the increased rate of glucose production and flow to peripheral tissue which characteristically occurs after thermal injury.", "PMID": 982249}
{"id": "pubmed23n0032_12957", "title": "Pulmonary insufficiency produced by isoproterenol.", "content": "Twelve healthy, anesthetized, mechanically ventilated dogs received an infusion of isoproterenol hydrochloride, 0.1 microgram per kilogram for two hours. There was a rapid and statistically significant increase in the pulmonary shunt, cardiac output and mean pulmonary artery pressure; a significant decrease in the pulmonary vascular resistance, and no significant change in the pulmonary artery wedge pressure. These findings support those of previous studies which demonstrated the beta adrenergic stimulation is important in the pathogenesis of pulmonary insufficiency. The shunt results from ventilation-perfusion inequalities produced by the increased pulmonary blood flow and pulmonary vasodilatation. Because of the production of pulmonary insufficiency, isoproterenol or other beta adrenergic agonists should be used with caution in the critically ill, who often have or may develop the respiratory distress syndrome.", "contents": "Pulmonary insufficiency produced by isoproterenol. Twelve healthy, anesthetized, mechanically ventilated dogs received an infusion of isoproterenol hydrochloride, 0.1 microgram per kilogram for two hours. There was a rapid and statistically significant increase in the pulmonary shunt, cardiac output and mean pulmonary artery pressure; a significant decrease in the pulmonary vascular resistance, and no significant change in the pulmonary artery wedge pressure. These findings support those of previous studies which demonstrated the beta adrenergic stimulation is important in the pathogenesis of pulmonary insufficiency. The shunt results from ventilation-perfusion inequalities produced by the increased pulmonary blood flow and pulmonary vasodilatation. Because of the production of pulmonary insufficiency, isoproterenol or other beta adrenergic agonists should be used with caution in the critically ill, who often have or may develop the respiratory distress syndrome.", "PMID": 982250}
{"id": "pubmed23n0032_12958", "title": "The incidence of solitary pulmonary metastasis from carcinoma of the large intestine.", "content": "Two hundred and two consecutive patients who underwent resection of an adenocarcinoma of the colon or rectum from January 1958 to December 1972 were evaluated for the development of a metastatic tumor in the lungs. In the 185 patients who survived the 30 day postoperative period, 30 malignant pulmonary lesions were subsequently recognized. Six of these were a solitary lesion and 24 were multiple lesions. Three of these malignant lesions were proved to be other than metastatic disease. Metastatic adenocarcinoma from the colon or rectum was proved to be present in 27 patients, an incidence of 14.5 per cent. Only four of these lesions were solitary, 14.8 per cent of the metastatic pulmonary lesions. The solitary metastatic tumors represented an incidence of 2.1 per cent in this patient population, but long term tumor-free survival was possible with appropriate pulmonary resection of the metastatic disease. It is suggested that periodic roentgenographic examination of the chest, every four to six months, be an integral part of the postoperative follow-up study carried out on these patients.", "contents": "The incidence of solitary pulmonary metastasis from carcinoma of the large intestine. Two hundred and two consecutive patients who underwent resection of an adenocarcinoma of the colon or rectum from January 1958 to December 1972 were evaluated for the development of a metastatic tumor in the lungs. In the 185 patients who survived the 30 day postoperative period, 30 malignant pulmonary lesions were subsequently recognized. Six of these were a solitary lesion and 24 were multiple lesions. Three of these malignant lesions were proved to be other than metastatic disease. Metastatic adenocarcinoma from the colon or rectum was proved to be present in 27 patients, an incidence of 14.5 per cent. Only four of these lesions were solitary, 14.8 per cent of the metastatic pulmonary lesions. The solitary metastatic tumors represented an incidence of 2.1 per cent in this patient population, but long term tumor-free survival was possible with appropriate pulmonary resection of the metastatic disease. It is suggested that periodic roentgenographic examination of the chest, every four to six months, be an integral part of the postoperative follow-up study carried out on these patients.", "PMID": 982251}
{"id": "pubmed23n0032_12959", "title": "Intravascular coagulation in surgical procedures on the abdominal aorta.", "content": "A prospective study was performed on 32 consecutive patients undergoing elective operations on the abdominal aorta. Dacron prosthetic grafts were used to replace resected abdominal aortic aneurysms or to bypass aorta-iliac occlusive disease. Complete coagulation studies were performed preoperatively, immediately postoperatively and 24 hours postoperatively. Twenty to 30 per cent of the patients had significant postoperative alterations in prothrombin time, partial thromboplastin time and platelet count. Fibrin monomer, fibrin split products and plasminogen were abnormal in 40 to 80 per cent of the patients postoperatively. Results of preoperative studies showed no significant abnormalities. One of the 32 patients had mild clinical evidence of disseminated intravascular coagulation postoperatively, which was treated with 5 units of heparin per kilogram per hour. Results of the study indicate that aortic grafting procedures frequently produce intravascular coagulation, either local or disseminated. In most patients, this is offset by activation of the fibrinolytic system. However, clinically significant sequelae may result, requiring prompt recognition and treatment.", "contents": "Intravascular coagulation in surgical procedures on the abdominal aorta. A prospective study was performed on 32 consecutive patients undergoing elective operations on the abdominal aorta. Dacron prosthetic grafts were used to replace resected abdominal aortic aneurysms or to bypass aorta-iliac occlusive disease. Complete coagulation studies were performed preoperatively, immediately postoperatively and 24 hours postoperatively. Twenty to 30 per cent of the patients had significant postoperative alterations in prothrombin time, partial thromboplastin time and platelet count. Fibrin monomer, fibrin split products and plasminogen were abnormal in 40 to 80 per cent of the patients postoperatively. Results of preoperative studies showed no significant abnormalities. One of the 32 patients had mild clinical evidence of disseminated intravascular coagulation postoperatively, which was treated with 5 units of heparin per kilogram per hour. Results of the study indicate that aortic grafting procedures frequently produce intravascular coagulation, either local or disseminated. In most patients, this is offset by activation of the fibrinolytic system. However, clinically significant sequelae may result, requiring prompt recognition and treatment.", "PMID": 982252}
{"id": "pubmed23n0032_12960", "title": "Operative cholangiography and special technical consideration in the management of severe injury of the liver.", "content": "The majority of hepatic injuries can be adequately managed by control of bleeding locally at the site, debridement and ample drainage. In some instances, severe blunt trauma and high velocity missile wounds may result in the disruption of intrahepatic structures and significant devitalization of the parenchyma of the liver, necessitating hepatic resection. Operative cholangiography was found to be useful in the evaluation and management of this type of severe injury to the liver. It is simple, practical method to recognize and localize a major disruption of the parenchyma of the liver and bile ducts; to help decide whether or not segmental, sublobar or labor hepatectomy should be performed, and to detect bile leaks from the divided bile ducts after resection of the liver. A modified technique for performing hepatic resection during an emergency situation was suggested to be more suitable than the classic technique. This is based on finger dissection along the line of injury and individual ligation of bile ducts and vessels as they are exposed within the parenchyma of the liver instead of isolation and ligation of the main inflow vessels and of the major ducts at the hilus and retrohepatic ligation of the hepatic veins.", "contents": "Operative cholangiography and special technical consideration in the management of severe injury of the liver. The majority of hepatic injuries can be adequately managed by control of bleeding locally at the site, debridement and ample drainage. In some instances, severe blunt trauma and high velocity missile wounds may result in the disruption of intrahepatic structures and significant devitalization of the parenchyma of the liver, necessitating hepatic resection. Operative cholangiography was found to be useful in the evaluation and management of this type of severe injury to the liver. It is simple, practical method to recognize and localize a major disruption of the parenchyma of the liver and bile ducts; to help decide whether or not segmental, sublobar or labor hepatectomy should be performed, and to detect bile leaks from the divided bile ducts after resection of the liver. A modified technique for performing hepatic resection during an emergency situation was suggested to be more suitable than the classic technique. This is based on finger dissection along the line of injury and individual ligation of bile ducts and vessels as they are exposed within the parenchyma of the liver instead of isolation and ligation of the main inflow vessels and of the major ducts at the hilus and retrohepatic ligation of the hepatic veins.", "PMID": 982253}
{"id": "pubmed23n0032_12961", "title": "Effect of norepinephrine on gastric wound healing.", "content": "Lavage of the stomach with norepinephrine has been suggested as a temporary means of controlling upper gastrointestinal tract bleeding. The effect of norepinephrine on the healing of a standard gastric incision in the rat was studied using irrigation topically. When compared with those rats in the control study that were irrigated with a saline solution alone, no significant difference was found in the tensile strength, local inflammatory response or synthesis of new collagen, as determined by the hydroxyproline-proline ratio. There would appear to be no adverse effect on gastric wound healing as a result of preliminary irrigation of the gastric mucosa with norepinephrine given topically in a concentration of 16 milligrams per liter.", "contents": "Effect of norepinephrine on gastric wound healing. Lavage of the stomach with norepinephrine has been suggested as a temporary means of controlling upper gastrointestinal tract bleeding. The effect of norepinephrine on the healing of a standard gastric incision in the rat was studied using irrigation topically. When compared with those rats in the control study that were irrigated with a saline solution alone, no significant difference was found in the tensile strength, local inflammatory response or synthesis of new collagen, as determined by the hydroxyproline-proline ratio. There would appear to be no adverse effect on gastric wound healing as a result of preliminary irrigation of the gastric mucosa with norepinephrine given topically in a concentration of 16 milligrams per liter.", "PMID": 982254}
{"id": "pubmed23n0032_12962", "title": "Status of the contralateral ovary in encapsulated low grade malignant tumors of the ovary.", "content": "The incidence of bilateral involvement in carcinoma of the ovary is shown to be relatively high. This is true for the different histologic type of malignant lesions and even when the ovary is grossly normal in appearance and on palpation. Only when carcinoma of the ovary is low grade, intracystic, unruptured and nonadherent and is in a young woman desirous of childbirth can it be managed conservatively.", "contents": "Status of the contralateral ovary in encapsulated low grade malignant tumors of the ovary. The incidence of bilateral involvement in carcinoma of the ovary is shown to be relatively high. This is true for the different histologic type of malignant lesions and even when the ovary is grossly normal in appearance and on palpation. Only when carcinoma of the ovary is low grade, intracystic, unruptured and nonadherent and is in a young woman desirous of childbirth can it be managed conservatively.", "PMID": 982255}
{"id": "pubmed23n0032_12963", "title": "Surgical management of colon atresia.", "content": "Colon atresia is a rare cause of intestinal obstruction in the neonate and requires early diagnosis and prompt surgical treatment. It is impossible in the neonate to differentiate colon atresia from other forms of obstruction at the time of initial presentation. The diagnosis is confirmed roentgenographically, including views of the abdomen and contrast barium enema series. Lesions proximal to the splenic flexure are treated with initial resection of the atretic segment and a primary anastomosis. Those lesions distal to the splentic flexure are managed initally with a diverting loop colostomy with subsequent staged resection and anastomosis.", "contents": "Surgical management of colon atresia. Colon atresia is a rare cause of intestinal obstruction in the neonate and requires early diagnosis and prompt surgical treatment. It is impossible in the neonate to differentiate colon atresia from other forms of obstruction at the time of initial presentation. The diagnosis is confirmed roentgenographically, including views of the abdomen and contrast barium enema series. Lesions proximal to the splenic flexure are treated with initial resection of the atretic segment and a primary anastomosis. Those lesions distal to the splentic flexure are managed initally with a diverting loop colostomy with subsequent staged resection and anastomosis.", "PMID": 982256}
{"id": "pubmed23n0032_12964", "title": "Side-effects of high pressure irrigation.", "content": "This study was undertaken to identify side-effects of high pressure irrigation. Standardized surgical wounds made in Yorkshire pigs were subjected to high pressure syringe and pulsatile irrigation. As a result of these treatments, fluids were disseminated into the adjacent tissue of the wound, predominantly in a lateral direction. Bacteria did not accompany this disseminated fluid and apparently were filtered out by the surface tissues. This treatment results in a tissue injury which impairs its defenses, making the wound more susceptible to infection. However, the remarkable cleansing capacity of high pressure irrigation appears to outweigh this side-effect, since heavily contaminated wounds subjected to this treatment heal per primum without infection.", "contents": "Side-effects of high pressure irrigation. This study was undertaken to identify side-effects of high pressure irrigation. Standardized surgical wounds made in Yorkshire pigs were subjected to high pressure syringe and pulsatile irrigation. As a result of these treatments, fluids were disseminated into the adjacent tissue of the wound, predominantly in a lateral direction. Bacteria did not accompany this disseminated fluid and apparently were filtered out by the surface tissues. This treatment results in a tissue injury which impairs its defenses, making the wound more susceptible to infection. However, the remarkable cleansing capacity of high pressure irrigation appears to outweigh this side-effect, since heavily contaminated wounds subjected to this treatment heal per primum without infection.", "PMID": 982257}
{"id": "pubmed23n0032_12965", "title": "Vascular permeability induced by pancreatic exudate formed during acute pancreatitis in dogs.", "content": "Acute pancreatitis was produced in five dogs by injecting bile into the pancreatic duct. The capillary permeability effects of the exudate formed within the peritoneal cavity were studied by injecting the exudate intradermally into puppies. The amount of radioactively labeled albumin escaping from the circulation and appearing at the intradermal injection site was used as a measure of capillary permeability. It was observed that the peritoneal exudate, especially that produced in the early stage of bile induced pancreatitis, contains one or more substances which result in an increased capillary permeability when injected intradermally into puppies.", "contents": "Vascular permeability induced by pancreatic exudate formed during acute pancreatitis in dogs. Acute pancreatitis was produced in five dogs by injecting bile into the pancreatic duct. The capillary permeability effects of the exudate formed within the peritoneal cavity were studied by injecting the exudate intradermally into puppies. The amount of radioactively labeled albumin escaping from the circulation and appearing at the intradermal injection site was used as a measure of capillary permeability. It was observed that the peritoneal exudate, especially that produced in the early stage of bile induced pancreatitis, contains one or more substances which result in an increased capillary permeability when injected intradermally into puppies.", "PMID": 982258}
{"id": "pubmed23n0032_12966", "title": "Diagnosis of gastrinoma by the secretin suppression test.", "content": "In contrast to normal physiologic feedback suppression of serum gastrin by secretin, a paradoxic rise in the serum gastrin level has been observed in patients with gastrinoma after the administration of exogenous secretin. Exploitation of this phenomenon in the differential diagnosis of gastrinoma has been restricted by limited individual experience. Serial serum specimens for gastrin radioimmunoassay were collected from 13 patients with histologically proved gastrinoma both before and after the administration of Boot's secretin, 3 units per kilogram. Thirty-nine others with histologically proved gastrinoma who had been studied with exogenous secretin were identified in the literature. Both the peak gastrin and the integrated gastrin responses were increased after secretin administration in each of the patients in this combined series, although the magnitude of the increase was small in four patients. The absence of physiologic suppression by secretin implies neoplastic autonomy of gastrin releasing sites. While an augmented gastrin response to secretin is commonly seen in patients with gastrinoma, from a physiologic standpoint, a lack of suppression constitutes a positive secretin suppression test. Accumulated experience is consistent and suggests that this test is an important adjunt in the differential diagnosis of hypergastrinemia.", "contents": "Diagnosis of gastrinoma by the secretin suppression test. In contrast to normal physiologic feedback suppression of serum gastrin by secretin, a paradoxic rise in the serum gastrin level has been observed in patients with gastrinoma after the administration of exogenous secretin. Exploitation of this phenomenon in the differential diagnosis of gastrinoma has been restricted by limited individual experience. Serial serum specimens for gastrin radioimmunoassay were collected from 13 patients with histologically proved gastrinoma both before and after the administration of Boot's secretin, 3 units per kilogram. Thirty-nine others with histologically proved gastrinoma who had been studied with exogenous secretin were identified in the literature. Both the peak gastrin and the integrated gastrin responses were increased after secretin administration in each of the patients in this combined series, although the magnitude of the increase was small in four patients. The absence of physiologic suppression by secretin implies neoplastic autonomy of gastrin releasing sites. While an augmented gastrin response to secretin is commonly seen in patients with gastrinoma, from a physiologic standpoint, a lack of suppression constitutes a positive secretin suppression test. Accumulated experience is consistent and suggests that this test is an important adjunt in the differential diagnosis of hypergastrinemia.", "PMID": 982259}
{"id": "pubmed23n0032_12967", "title": "Safety and efficacy of the antiseptic chlorhexidine gluconate.", "content": "Chlorhexidine gluconate, an antiseptic for the skin, has recently been investigated in a series of clinical studies on its safety and efficacy. By using standard methods, Hibiclens, Hibitane tinted tincture and 0.5 per cent aqueous chlorhexidine gluconate were shown to have an extremely low potential for the production of irritation, allergic contact sensitization, photoallergic contact sensitization and phototoxicity. In the glove fluid test for efficacy against resident flora of the hand, Hibiclens produced log10 reductions over the control of 1.9398, 2.5371 and 2.6885 for test days 1, 2 and 5, respectively. Corresponding reductions for Hibitane tinted tincture were 3.6903, 4.0984 and 4.1253 and for the aqueous formulation, 1.5003, 1.5721 and 1.8692. In a transient flora skin contamination study, Serratia marcescens was applied at an average level of 6.8363 log10 organisms per milliliter to persons' hands, after which a 15 second Hibiclens hand wash was performed. Following five of these contaminations and hand washes, there was an over-all log10 reduction in recoverable Serratia of 3.8500. Counts were further determined after ten, 15, 20 and 25 contaminations and hand washes, resulting in corresponding reductions of 4.2649, 4.6661, 4.8501 and 5.1725, respectively. Chlorhexidine gluconate offers an alternative to available antiseptics for the skin. It has been shown to be a fast acting, broad spectrum antimicrobial agent, with an extremely low potential for eliciting dermal reactions.", "contents": "Safety and efficacy of the antiseptic chlorhexidine gluconate. Chlorhexidine gluconate, an antiseptic for the skin, has recently been investigated in a series of clinical studies on its safety and efficacy. By using standard methods, Hibiclens, Hibitane tinted tincture and 0.5 per cent aqueous chlorhexidine gluconate were shown to have an extremely low potential for the production of irritation, allergic contact sensitization, photoallergic contact sensitization and phototoxicity. In the glove fluid test for efficacy against resident flora of the hand, Hibiclens produced log10 reductions over the control of 1.9398, 2.5371 and 2.6885 for test days 1, 2 and 5, respectively. Corresponding reductions for Hibitane tinted tincture were 3.6903, 4.0984 and 4.1253 and for the aqueous formulation, 1.5003, 1.5721 and 1.8692. In a transient flora skin contamination study, Serratia marcescens was applied at an average level of 6.8363 log10 organisms per milliliter to persons' hands, after which a 15 second Hibiclens hand wash was performed. Following five of these contaminations and hand washes, there was an over-all log10 reduction in recoverable Serratia of 3.8500. Counts were further determined after ten, 15, 20 and 25 contaminations and hand washes, resulting in corresponding reductions of 4.2649, 4.6661, 4.8501 and 5.1725, respectively. Chlorhexidine gluconate offers an alternative to available antiseptics for the skin. It has been shown to be a fast acting, broad spectrum antimicrobial agent, with an extremely low potential for eliciting dermal reactions.", "PMID": 982260}
{"id": "pubmed23n0032_12968", "title": "A technique for preparation of saphenous vein grafts.", "content": "Late stenoses in vein grafts can result from trauma to the adventitia and the endothelium and to drying of the vein graft during preparation. Since vein grafts are sensitive to trauma, it is best to minimize nonphysiologic factors, that is, to avoid excessive hydrostatic pressure and excessive handling with instruments. With this method, the vein is prepared for distal anastomosis by distention at arterial pressure using blood as the physiologic solution. It avoids drying or prolonged storage under deleterious conditions while preparing for the anastomosis and shortens total operating time. Potential damage which can impair the efficiency of the vein as a conduit for blood is minimized.", "contents": "A technique for preparation of saphenous vein grafts. Late stenoses in vein grafts can result from trauma to the adventitia and the endothelium and to drying of the vein graft during preparation. Since vein grafts are sensitive to trauma, it is best to minimize nonphysiologic factors, that is, to avoid excessive hydrostatic pressure and excessive handling with instruments. With this method, the vein is prepared for distal anastomosis by distention at arterial pressure using blood as the physiologic solution. It avoids drying or prolonged storage under deleterious conditions while preparing for the anastomosis and shortens total operating time. Potential damage which can impair the efficiency of the vein as a conduit for blood is minimized.", "PMID": 982263}
{"id": "pubmed23n0032_12969", "title": "Eye infections with herpes simplex viruses in neonates.", "content": "A newborn with severe ocular herpes simplex virus (HSV) type 2 infection acquired in utero is presented to exemplify problems in diagnosis and management. A review of 297 newborns with HSV type 1 or type 2 infection reveals that about one-fifth demonstrate ocular involvement including one or more of the following: microphatalmia, conjunctivitis, keratitis, chorioretinitis, optic neuritis and cataracts.", "contents": "Eye infections with herpes simplex viruses in neonates. A newborn with severe ocular herpes simplex virus (HSV) type 2 infection acquired in utero is presented to exemplify problems in diagnosis and management. A review of 297 newborns with HSV type 1 or type 2 infection reveals that about one-fifth demonstrate ocular involvement including one or more of the following: microphatalmia, conjunctivitis, keratitis, chorioretinitis, optic neuritis and cataracts.", "PMID": 982267}
{"id": "pubmed23n0032_12970", "title": "Type 2 herpes simplex virus infections.", "content": "The problems related to herpes simplex virus (HSV) type 2 infections include: (1) the clinical diseases produced primarily at urogenital sites, but also in extragenital areas, and the frequent recurrences of such infections; (2) the severity of the diseases produced in immunocompromised hosts and in newborns, including a varity of ocular manifestations; and (3) the possible role of the virus in human cancers. The usually sexually transmitted mode of spread of this virus has increased current medical concern with this virus. Although laboratory diagnosis of HSV-2 infection is currently available, we still lack effective preventive or therapeutic means for most clinical forms of the infection. However, the great progress made over the past decade in the molecular, virological, immunological and clinicoepidemiological aspects of HSV are providing the necessary tools to attain this goal.", "contents": "Type 2 herpes simplex virus infections. The problems related to herpes simplex virus (HSV) type 2 infections include: (1) the clinical diseases produced primarily at urogenital sites, but also in extragenital areas, and the frequent recurrences of such infections; (2) the severity of the diseases produced in immunocompromised hosts and in newborns, including a varity of ocular manifestations; and (3) the possible role of the virus in human cancers. The usually sexually transmitted mode of spread of this virus has increased current medical concern with this virus. Although laboratory diagnosis of HSV-2 infection is currently available, we still lack effective preventive or therapeutic means for most clinical forms of the infection. However, the great progress made over the past decade in the molecular, virological, immunological and clinicoepidemiological aspects of HSV are providing the necessary tools to attain this goal.", "PMID": 982268}
{"id": "pubmed23n0032_12971", "title": "Alterations in volatile free fatty acids of blood after hepatectomy.", "content": "Volatile free fatty acids (VFA) in blood increased approximately twofold in dogs subjected to total hepatectomy. The average total plasma VFA preoperatively was 1,585 mug percent and shortly before death, postoperatively, was 2,798 mug percent. The corresponding red cell concentrations were essentially the same. Acetic acid was 81 percent of the total VFA, propionic acid 7 percent, isobutyric acid 4 percent, butyric acid one percent, and isovaleric acid 5 percent. There was little or no isovalerate in red cells. The increments in the individual fatty acids after hepatectomy were highly variable, but the average increase with time was almost linear. The increase in VFA probably reflects an increased utilization of the branched-chain aminoacids by extrahepatic tissues.", "contents": "Alterations in volatile free fatty acids of blood after hepatectomy. Volatile free fatty acids (VFA) in blood increased approximately twofold in dogs subjected to total hepatectomy. The average total plasma VFA preoperatively was 1,585 mug percent and shortly before death, postoperatively, was 2,798 mug percent. The corresponding red cell concentrations were essentially the same. Acetic acid was 81 percent of the total VFA, propionic acid 7 percent, isobutyric acid 4 percent, butyric acid one percent, and isovaleric acid 5 percent. There was little or no isovalerate in red cells. The increments in the individual fatty acids after hepatectomy were highly variable, but the average increase with time was almost linear. The increase in VFA probably reflects an increased utilization of the branched-chain aminoacids by extrahepatic tissues.", "PMID": 982272}
{"id": "pubmed23n0032_12972", "title": "Experimental herpetic keratitis in the guinea pig.", "content": "A model of herpetic keratitis with type 1 Herpesvirus hominis was established in the guinea pig. The administration of 10 mg triamcinolone acetonide subconjunctivally in both eyes resulted in an amelioration of the clinical course of the disease process, whereas 40 mg injected peritoneally did not. The possible pathophysiology of these responses is discussed.", "contents": "Experimental herpetic keratitis in the guinea pig. A model of herpetic keratitis with type 1 Herpesvirus hominis was established in the guinea pig. The administration of 10 mg triamcinolone acetonide subconjunctivally in both eyes resulted in an amelioration of the clinical course of the disease process, whereas 40 mg injected peritoneally did not. The possible pathophysiology of these responses is discussed.", "PMID": 982269}
{"id": "pubmed23n0032_12973", "title": "Treatment of biliary atresia: microsurgery for hepatic portoenterostomy.", "content": "Microsurgery was used for hepatic portoenterostomy as the treatment of biliary atresia in 46 patients whom we treated since 1972. The microscope had a higher power (eight or ten times) magnification. As for the results of the operative procedure with the microscope, of 46 patients, 32 (70 percent) showed satisfactory bile flow postoperatively.", "contents": "Treatment of biliary atresia: microsurgery for hepatic portoenterostomy. Microsurgery was used for hepatic portoenterostomy as the treatment of biliary atresia in 46 patients whom we treated since 1972. The microscope had a higher power (eight or ten times) magnification. As for the results of the operative procedure with the microscope, of 46 patients, 32 (70 percent) showed satisfactory bile flow postoperatively.", "PMID": 982273}
{"id": "pubmed23n0032_12974", "title": "Feline herpes virus infection.", "content": "Ten cases of naturally occurring dendritic keratitis (feline herpes keratitis), which are manifestations of the disease commonly known as feline viral rhinotracheitis, have been identified in domestic cats in San Fancisco. Neonatal, juvenile, and adult forms of the disease are described and their treatment is discussed.", "contents": "Feline herpes virus infection. Ten cases of naturally occurring dendritic keratitis (feline herpes keratitis), which are manifestations of the disease commonly known as feline viral rhinotracheitis, have been identified in domestic cats in San Fancisco. Neonatal, juvenile, and adult forms of the disease are described and their treatment is discussed.", "PMID": 982270}
{"id": "pubmed23n0032_12975", "title": "Cephalic vein cross-over bypass for subclavian vein thrombosis: a case report.", "content": "A case of axillary vein thrombosis in a young man was treated by means of a cephalic vein cross-over bypass graft. A temporary arteriovenous fistula performed in the antecubital fossa resulted in a reasonable enlargement of the graft and prevented its occlusion in the critical postoperative period until healing of the intima resulted. The swelling disappeared and the patient, who was completely incapacitated functionally prior to the operation, resumed normal activity.", "contents": "Cephalic vein cross-over bypass for subclavian vein thrombosis: a case report. A case of axillary vein thrombosis in a young man was treated by means of a cephalic vein cross-over bypass graft. A temporary arteriovenous fistula performed in the antecubital fossa resulted in a reasonable enlargement of the graft and prevented its occlusion in the critical postoperative period until healing of the intima resulted. The swelling disappeared and the patient, who was completely incapacitated functionally prior to the operation, resumed normal activity.", "PMID": 982274}
{"id": "pubmed23n0032_12976", "title": "Herpes simplex: the primary infection.", "content": "A primary infection with herpes simplex virus (HSV) occurs at some time in the life of almost every member of the population, especially among those living in crowded, unsanitary conditions. In many cases the lesions are subclinical, and in most cases they are self-limited even when clinical. In a few patients, such as the newborn infant, the patient with concomitant eczema, or the patient with reduced immunity, the primary infection may become severe, generalized, and life-threatening. Type 1 HSV has a predilection for a number of sites, including the oral cavity, the eye, and the skin; and type 2 HSV has a predilection for the genital area and for the newborn.", "contents": "Herpes simplex: the primary infection. A primary infection with herpes simplex virus (HSV) occurs at some time in the life of almost every member of the population, especially among those living in crowded, unsanitary conditions. In many cases the lesions are subclinical, and in most cases they are self-limited even when clinical. In a few patients, such as the newborn infant, the patient with concomitant eczema, or the patient with reduced immunity, the primary infection may become severe, generalized, and life-threatening. Type 1 HSV has a predilection for a number of sites, including the oral cavity, the eye, and the skin; and type 2 HSV has a predilection for the genital area and for the newborn.", "PMID": 982271}
{"id": "pubmed23n0032_12977", "title": "Splenic artery ligation in experimental hypersplenism.", "content": "The effects of splenic artery ligation (SAL) were examined in Sprague-Dawley rats with methyl-cellulose-induced hypersplenism. When performed close to the splenic hilium, SAL effectively reduced functioning splenic mass and raised peripheral counts of leukocytes and platelets, a response similar to that following total splenectomy. In hypersplenism, therefore, a satisfactory hematologic response may not necessary require total ablation of the spleen but merely substantial reduction of functioning splenic tissue.", "contents": "Splenic artery ligation in experimental hypersplenism. The effects of splenic artery ligation (SAL) were examined in Sprague-Dawley rats with methyl-cellulose-induced hypersplenism. When performed close to the splenic hilium, SAL effectively reduced functioning splenic mass and raised peripheral counts of leukocytes and platelets, a response similar to that following total splenectomy. In hypersplenism, therefore, a satisfactory hematologic response may not necessary require total ablation of the spleen but merely substantial reduction of functioning splenic tissue.", "PMID": 982275}
{"id": "pubmed23n0032_12978", "title": "Failure to pentagastrin administration to restore postprandial acid secretion from Heidenhain pouches after antrectomy in dogs.", "content": "In four dogs with Heidenhain pouche, acid outputs from the pouch were measured in response to feeding boiled liver with or without simultaneous administration of pentagastrin. Antrectomy was performed and the experiments were repeated. Antrectomy decreased the acid response to feeding by 90 percent. The preantrectomy response was restored by infusing pentagastrin but near maximal doses were needed and the maximal response to feeding plus pentagastrin could not be restored. These results indicate that the decrease of the acid secretory response to feeding from Heidenhain pouches after antrectomy cannot be explained fully through suppression of endogenous gastrin release. Antrectomy probably interferes with the intestinal phase of gastric secretion.", "contents": "Failure to pentagastrin administration to restore postprandial acid secretion from Heidenhain pouches after antrectomy in dogs. In four dogs with Heidenhain pouche, acid outputs from the pouch were measured in response to feeding boiled liver with or without simultaneous administration of pentagastrin. Antrectomy was performed and the experiments were repeated. Antrectomy decreased the acid response to feeding by 90 percent. The preantrectomy response was restored by infusing pentagastrin but near maximal doses were needed and the maximal response to feeding plus pentagastrin could not be restored. These results indicate that the decrease of the acid secretory response to feeding from Heidenhain pouches after antrectomy cannot be explained fully through suppression of endogenous gastrin release. Antrectomy probably interferes with the intestinal phase of gastric secretion.", "PMID": 982276}
{"id": "pubmed23n0032_12979", "title": "Effect of histamine on technetium-99m excretion by gastric mucosa.", "content": "The ability of histamine to increase excretion of 99mtechnetium by gastric mucosa was investigated in dogs with Heidenhain pouches and denervated antral pouches. Histamine increased Heidenhain pouch 99mtechnetium output in a dose-related manner, and 99mtechnetium output was related linearly to acid output. Antral pouch 99mtechnetium output was unchanged by increasing doses of histamine. The study suggests that concomitant use of histamine may improve the accuracy of 99mtechnetium scanning in the clinical diagnosis of conditions caused by ectopic gastric mucosa.", "contents": "Effect of histamine on technetium-99m excretion by gastric mucosa. The ability of histamine to increase excretion of 99mtechnetium by gastric mucosa was investigated in dogs with Heidenhain pouches and denervated antral pouches. Histamine increased Heidenhain pouch 99mtechnetium output in a dose-related manner, and 99mtechnetium output was related linearly to acid output. Antral pouch 99mtechnetium output was unchanged by increasing doses of histamine. The study suggests that concomitant use of histamine may improve the accuracy of 99mtechnetium scanning in the clinical diagnosis of conditions caused by ectopic gastric mucosa.", "PMID": 982277}
{"id": "pubmed23n0032_12980", "title": "A microsurgical technique for orthotopic ovarian transplantation in the Chinese hamster.", "content": "A microscopic surgical technique for the orthotopic transplantation of ovaries in the Chinese hamster is described. The procedure, which uses microcauterization of the mesovarian stump and closure of the ovarian bursa with microvascular suture, resulted in a 75 to 80 percent surgical success rate in a series of 12 nondiabetic and 12 diabetic hamsters. Applications include the production of prediabetic offspring, the study of intrauterine environmental effects on transplant-derived offspring, and the preservation of endangered genetic colony sublines.", "contents": "A microsurgical technique for orthotopic ovarian transplantation in the Chinese hamster. A microscopic surgical technique for the orthotopic transplantation of ovaries in the Chinese hamster is described. The procedure, which uses microcauterization of the mesovarian stump and closure of the ovarian bursa with microvascular suture, resulted in a 75 to 80 percent surgical success rate in a series of 12 nondiabetic and 12 diabetic hamsters. Applications include the production of prediabetic offspring, the study of intrauterine environmental effects on transplant-derived offspring, and the preservation of endangered genetic colony sublines.", "PMID": 982278}
{"id": "pubmed23n0032_12981", "title": "Onset of cell proliferation in the shortened gut: growth after subtotal colectomy.", "content": "Cell proliferation and remodeling of the small intestine were studied after subtotal colectomy in the rat. After an initial fall in nucleic acid contents, there was a trend to increasing amounts of RNA throughout the intestine on the fourteenth postoperative day; by the thirtieth day RNA and DNA contents were increased about 20 percent. DNA synthesis increased after 5 days. Although initially villi became shorter and crypts shallower, higher villi, deeper crypts, and an increased rate of cell migration were present after 14 days. Subtotal colectomy stimulates nucleic acid synthesis and remodeling of the small bowel within 2 weeks. Changes are more marked in ileum.", "contents": "Onset of cell proliferation in the shortened gut: growth after subtotal colectomy. Cell proliferation and remodeling of the small intestine were studied after subtotal colectomy in the rat. After an initial fall in nucleic acid contents, there was a trend to increasing amounts of RNA throughout the intestine on the fourteenth postoperative day; by the thirtieth day RNA and DNA contents were increased about 20 percent. DNA synthesis increased after 5 days. Although initially villi became shorter and crypts shallower, higher villi, deeper crypts, and an increased rate of cell migration were present after 14 days. Subtotal colectomy stimulates nucleic acid synthesis and remodeling of the small bowel within 2 weeks. Changes are more marked in ileum.", "PMID": 982279}
{"id": "pubmed23n0032_12982", "title": "Role of serum nonsuppressible insulin-like activity (NSILA) in wound healing. I. Influence of thyroparathyroidectomy on serum NSILA and wound healing in the rat.", "content": "Serum contains a 90,000 molecular weight glycoprotein that exhibits insulin-like activity on adipocytes, skeletal muscle, and fibroblasts in tissue culture; however, this protein is physicochemically and immunochemically distinct from insulin and presently is termed \"nonsuppressible insulin-like activity (\"nsilA).\" This study was designed to assess the response of serum NSILA to thyroparathyroidectomy (TPTHX) and to associate this response with tissue repair processes in the injured rat. It was postulated that NSILA modulates the fibroblastic response in wound healing. TPTHX decreased NSILA to 40 percent of control levels (p less than 0.001); 90 male animals subsequently were wounded by either a 10 percent third-degree burn or skin excision. Following injury, NSILA significantly increased in both control and TPTHX groups, but this acute-phase response was attenuated in TPTHX animals. Light microscopy of granulation tissue demonstrated a diminution in the fibroblastic response in TPTHX animals. Hydroxyproline analysis of granulation tissue revealed a significant decrease (p less than 0.025) in collagen content in TPTHX animals with low NSILA levels. The results suggest that serum NSILA levels are controlled, in part, by thyroid hormone and that NSILA may modulate the fibroblastic response of connective tissue repair processes.", "contents": "Role of serum nonsuppressible insulin-like activity (NSILA) in wound healing. I. Influence of thyroparathyroidectomy on serum NSILA and wound healing in the rat. Serum contains a 90,000 molecular weight glycoprotein that exhibits insulin-like activity on adipocytes, skeletal muscle, and fibroblasts in tissue culture; however, this protein is physicochemically and immunochemically distinct from insulin and presently is termed \"nonsuppressible insulin-like activity (\"nsilA).\" This study was designed to assess the response of serum NSILA to thyroparathyroidectomy (TPTHX) and to associate this response with tissue repair processes in the injured rat. It was postulated that NSILA modulates the fibroblastic response in wound healing. TPTHX decreased NSILA to 40 percent of control levels (p less than 0.001); 90 male animals subsequently were wounded by either a 10 percent third-degree burn or skin excision. Following injury, NSILA significantly increased in both control and TPTHX groups, but this acute-phase response was attenuated in TPTHX animals. Light microscopy of granulation tissue demonstrated a diminution in the fibroblastic response in TPTHX animals. Hydroxyproline analysis of granulation tissue revealed a significant decrease (p less than 0.025) in collagen content in TPTHX animals with low NSILA levels. The results suggest that serum NSILA levels are controlled, in part, by thyroid hormone and that NSILA may modulate the fibroblastic response of connective tissue repair processes.", "PMID": 982280}
{"id": "pubmed23n0032_12983", "title": "The role of house staff in undergraduate surgical education.", "content": "An analysis of the contribution made by house officers in fulfilling the objectives for a comprehensive clerkship experience was undertaken by questioning members of the 1975 medical class. The objectives examined over a broad spectrum of surgical disease and are applicable to any general surgical clerkship. In addition, the instruction received in technical skills was analyzed and deficiencies were noted. Major subject areas related to surgical disease were examined for adequacy of house staff instruction, and an estimation of hours per week devoted to the clerkship experience by house and staff was derived. The university-private hospital training program at this institution permitted a comparison of instruction between these clerkship settings. Some deficiencies did appear in intellectual and technical instruction in both clerkship environments. These deficiencies, however, usually were related to a paucity of patient material and to the lack of opportunity for clinical instruction which ensues from these cases. With few exceptions, the house staff provided nearly one third of the knowledge base acquired during the junior medical student experience. The results indicate that very little difference exists between the didactic role of the house officer in either the university or community hospital environment.", "contents": "The role of house staff in undergraduate surgical education. An analysis of the contribution made by house officers in fulfilling the objectives for a comprehensive clerkship experience was undertaken by questioning members of the 1975 medical class. The objectives examined over a broad spectrum of surgical disease and are applicable to any general surgical clerkship. In addition, the instruction received in technical skills was analyzed and deficiencies were noted. Major subject areas related to surgical disease were examined for adequacy of house staff instruction, and an estimation of hours per week devoted to the clerkship experience by house and staff was derived. The university-private hospital training program at this institution permitted a comparison of instruction between these clerkship settings. Some deficiencies did appear in intellectual and technical instruction in both clerkship environments. These deficiencies, however, usually were related to a paucity of patient material and to the lack of opportunity for clinical instruction which ensues from these cases. With few exceptions, the house staff provided nearly one third of the knowledge base acquired during the junior medical student experience. The results indicate that very little difference exists between the didactic role of the house officer in either the university or community hospital environment.", "PMID": 982281}
{"id": "pubmed23n0032_12984", "title": "Thiabendazole: a potential adjuvant in cancer therapy.", "content": "This set of experiments was designed to evaluate the effects of thiabendazole (TBZ), a relatively nontoxic thiazole derivative, on the delayed hypersensitivity response of normal mice, tumor-bearing mice, and mice immunosuppressed by radiation and chemotherapy. Normal mice treated with a single intraperitoneal injection (20 mg. per kilogram) of TBZ given on the day of challenge with 2,4 dinitrochlorobenzene (DNCB) had 41 percent greater swelling of the injected foot pad than did control animals. This increased immune reactivity could be transferred with spleen cells from TBZ-treated animals as well as with macrophages incubated in vitro with TBZ. Mice given sublethal radiation (450 r) had almost complete restoration of the delayed hypersensitivity response (90 percent of control) when treated with TBZ. The immunosuppressive effects of Adriamycin also could be reversed in a similar fashion. It is likely that the immune function of more than one population of spleen cells is amplified by TBZ treatment. The observed effects strongly suggest that thiabendazole may have a role as an adjunct in cancer therapy.", "contents": "Thiabendazole: a potential adjuvant in cancer therapy. This set of experiments was designed to evaluate the effects of thiabendazole (TBZ), a relatively nontoxic thiazole derivative, on the delayed hypersensitivity response of normal mice, tumor-bearing mice, and mice immunosuppressed by radiation and chemotherapy. Normal mice treated with a single intraperitoneal injection (20 mg. per kilogram) of TBZ given on the day of challenge with 2,4 dinitrochlorobenzene (DNCB) had 41 percent greater swelling of the injected foot pad than did control animals. This increased immune reactivity could be transferred with spleen cells from TBZ-treated animals as well as with macrophages incubated in vitro with TBZ. Mice given sublethal radiation (450 r) had almost complete restoration of the delayed hypersensitivity response (90 percent of control) when treated with TBZ. The immunosuppressive effects of Adriamycin also could be reversed in a similar fashion. It is likely that the immune function of more than one population of spleen cells is amplified by TBZ treatment. The observed effects strongly suggest that thiabendazole may have a role as an adjunct in cancer therapy.", "PMID": 982282}
{"id": "pubmed23n0032_12985", "title": "Monitoring of intraoperative heparinization in vascular surgery.", "content": "In 15 patients undergoing aortofemoral bypass, partial thromboplastin time (PTT) tests before and following intravenous administration of 75 U. per kilogram of heparin at zero, 30, 60, 90, and 120 minutes were determined for study of control of anticoagulant adequacy. The results demonstrate clearly that this amount provides excellent protection against thrombosis without bleeding complications. For intraoperative assay of heparin level effectiveness, the PTT test is advised. This test showed that a value of 250 percent of control still existed 75 minutes following the administration of heparin.", "contents": "Monitoring of intraoperative heparinization in vascular surgery. In 15 patients undergoing aortofemoral bypass, partial thromboplastin time (PTT) tests before and following intravenous administration of 75 U. per kilogram of heparin at zero, 30, 60, 90, and 120 minutes were determined for study of control of anticoagulant adequacy. The results demonstrate clearly that this amount provides excellent protection against thrombosis without bleeding complications. For intraoperative assay of heparin level effectiveness, the PTT test is advised. This test showed that a value of 250 percent of control still existed 75 minutes following the administration of heparin.", "PMID": 982283}
{"id": "pubmed23n0032_12986", "title": "Intravenous rupture of arteriosclerotic aneurysms of the abdominal aorta.", "content": "Intravenous rupture of abdominal aortic aneurysms occurs infrequently but should be considered with the coexistence of severe congestive failure, anasarca, and abdominal bruits. Six patients are presented with four survivors. In only two patients was the diagnosis considered preoperatively without angiography. Two were variants in that thrombus occluded the fistula, thereby negating findings usually manifested clinically. Diagnosis of this type can be made only during operation when copious venous bleeding ensues with evacuation of the aortic thrombus. Careful fluid management and prompt surgery are prerequisites to obtaining a successful outcome. Repair is accomplished easily by suturing the fistula from the aortic aspect, but care is required to avoid dislodgement of thrombus and atherosclerotic debris resulting in pulmonary embolism.", "contents": "Intravenous rupture of arteriosclerotic aneurysms of the abdominal aorta. Intravenous rupture of abdominal aortic aneurysms occurs infrequently but should be considered with the coexistence of severe congestive failure, anasarca, and abdominal bruits. Six patients are presented with four survivors. In only two patients was the diagnosis considered preoperatively without angiography. Two were variants in that thrombus occluded the fistula, thereby negating findings usually manifested clinically. Diagnosis of this type can be made only during operation when copious venous bleeding ensues with evacuation of the aortic thrombus. Careful fluid management and prompt surgery are prerequisites to obtaining a successful outcome. Repair is accomplished easily by suturing the fistula from the aortic aspect, but care is required to avoid dislodgement of thrombus and atherosclerotic debris resulting in pulmonary embolism.", "PMID": 982284}
{"id": "pubmed23n0032_12987", "title": "Cystadenocarcinoma of the pancreas presenting as a splenic cyst.", "content": "A report of a cystadenocarcinoma of the pancreas arising in the spleen from pancreatic heterotopia is presented. The fact that the resected pancreatic tail was microscopically normal and separate from the tumor lends strong support that the tumor arose from pancreatic heterotopia rather than from direct extension or metastasis.", "contents": "Cystadenocarcinoma of the pancreas presenting as a splenic cyst. A report of a cystadenocarcinoma of the pancreas arising in the spleen from pancreatic heterotopia is presented. The fact that the resected pancreatic tail was microscopically normal and separate from the tumor lends strong support that the tumor arose from pancreatic heterotopia rather than from direct extension or metastasis.", "PMID": 982285}
{"id": "pubmed23n0032_12988", "title": "Ca-DTPA-induced fetal death and malformation in mice.", "content": "Increased fetal mortality and congenital malformation were induced in mice by Ca-DTPA, a chelate widely used for the removal of internally deposited plutonium in human beings. Mated C57BL/Do female mice were given five daily injections of 720-2,880 mumol/kg Ca-DTPA during early, mid, or late gestation. Damage probably occurred as a result of the depletion by Ca-DTPA of zinc and manganese, trace elements essential to growth and development. By extension, it seems likely that Ca-DTPA could damage human fetuses. A much safer chelate, Zn-DTPA, is recommended for the treatment of accidental plutonium contamination.", "contents": "Ca-DTPA-induced fetal death and malformation in mice. Increased fetal mortality and congenital malformation were induced in mice by Ca-DTPA, a chelate widely used for the removal of internally deposited plutonium in human beings. Mated C57BL/Do female mice were given five daily injections of 720-2,880 mumol/kg Ca-DTPA during early, mid, or late gestation. Damage probably occurred as a result of the depletion by Ca-DTPA of zinc and manganese, trace elements essential to growth and development. By extension, it seems likely that Ca-DTPA could damage human fetuses. A much safer chelate, Zn-DTPA, is recommended for the treatment of accidental plutonium contamination.", "PMID": 982309}
{"id": "pubmed23n0032_12989", "title": "Reduced interlitter variability in rats resulting from a restricted mating period, and reassessment of the \"\"litter effect''.", "content": "Rats were mated for two or 15 hours and variability of day-12-embryos in weight, protein content, and [3H]thymidine incorporation was compared in the long mating period (LMP) and short mating period (SMP) groups by a 2-level nested analysis of variance. Variability in day-20 fetal weight was similarly compared. In both groups day-12 embryonic weight was relatively more variable than day-20 fetal weight, and variability was less in SMP than LMP animals for each comparison made, although statistical significance was attained only for thymidine incorporation. \"\"Litter effects'' were noted but not of the magnitude reported by other investigators. It was concluded that inappropriate statistical methods have encouraged the belief that among-litter variability usually exceeds within-litter fetal weight variability. The teratological implications of reduced development variability and the \"\"litter effect'' are discussed.", "contents": "Reduced interlitter variability in rats resulting from a restricted mating period, and reassessment of the \"\"litter effect''. Rats were mated for two or 15 hours and variability of day-12-embryos in weight, protein content, and [3H]thymidine incorporation was compared in the long mating period (LMP) and short mating period (SMP) groups by a 2-level nested analysis of variance. Variability in day-20 fetal weight was similarly compared. In both groups day-12 embryonic weight was relatively more variable than day-20 fetal weight, and variability was less in SMP than LMP animals for each comparison made, although statistical significance was attained only for thymidine incorporation. \"\"Litter effects'' were noted but not of the magnitude reported by other investigators. It was concluded that inappropriate statistical methods have encouraged the belief that among-litter variability usually exceeds within-litter fetal weight variability. The teratological implications of reduced development variability and the \"\"litter effect'' are discussed.", "PMID": 982310}
{"id": "pubmed23n0032_12990", "title": "Placental morphology in spontaneous human abortuses with normal and abnormal karyotypes.", "content": "A systematic gross and microscopic study of the placenta was done on human spontaneous abortions, correlating placental morphology and karyotype of the conceptus. Consistent abnormalities were seen in the four major forms of human heteroploidy, so that a fairly accurate \"\"karyotypic diagnosis'' could be made from careful gross and microscopic examination of the aborted material. Depressed growth activity and dysmorphogenesis characterized these placentas, reflecting placental participation in the disturbed phenotypic expression of heteroploidy.", "contents": "Placental morphology in spontaneous human abortuses with normal and abnormal karyotypes. A systematic gross and microscopic study of the placenta was done on human spontaneous abortions, correlating placental morphology and karyotype of the conceptus. Consistent abnormalities were seen in the four major forms of human heteroploidy, so that a fairly accurate \"\"karyotypic diagnosis'' could be made from careful gross and microscopic examination of the aborted material. Depressed growth activity and dysmorphogenesis characterized these placentas, reflecting placental participation in the disturbed phenotypic expression of heteroploidy.", "PMID": 982311}
{"id": "pubmed23n0032_12991", "title": "Preventive effect of riboflavin and ATP on the teratogenic effects of the phenothiazine derivative T-82.", "content": "To examine the hypothesis that the teratogenic effects of the phenothiazine derivative T-82 are due to its causing a riboflavin deficiency day 11 or 12 pregnant CB Wistar rats were each given 2,000 mg/kg T-82 po plus 100 mg/kg riboflavin ip, 12.4 mg/kg ATP ip, or both. The rates of fetal mortality and external malformations were significantly decreased in all supplementation experiments. The frequencies of cleft palate, micrognathia, and micromelia were unchanged but those of ectopic testis and hydrops fetalis were significantly increased in the group treated with T-82 and ATP on day 12.", "contents": "Preventive effect of riboflavin and ATP on the teratogenic effects of the phenothiazine derivative T-82. To examine the hypothesis that the teratogenic effects of the phenothiazine derivative T-82 are due to its causing a riboflavin deficiency day 11 or 12 pregnant CB Wistar rats were each given 2,000 mg/kg T-82 po plus 100 mg/kg riboflavin ip, 12.4 mg/kg ATP ip, or both. The rates of fetal mortality and external malformations were significantly decreased in all supplementation experiments. The frequencies of cleft palate, micrognathia, and micromelia were unchanged but those of ectopic testis and hydrops fetalis were significantly increased in the group treated with T-82 and ATP on day 12.", "PMID": 982312}
{"id": "pubmed23n0032_12992", "title": "Studies of teratogenic effects of the dithiocarbamates maneb, mancozeb, and propineb.", "content": "Oral administration of high dosages of the dithiocarbamate pesticides maneb and mancozeb was teratogenic in rats but not in mice. The malformations, severe limb and craniofacial defects, were pronounced after maneb treatment but less so after mancozeb and propineb, zinc-containing compounds. The teratogenic effect of maneb was progressively reduced by simultaneously administering increasing amounts of zinc acetate. The mechanism of the teratogenic effect may involve the compounds being chelating agents, trapping zinc required for many important enzyme systems.", "contents": "Studies of teratogenic effects of the dithiocarbamates maneb, mancozeb, and propineb. Oral administration of high dosages of the dithiocarbamate pesticides maneb and mancozeb was teratogenic in rats but not in mice. The malformations, severe limb and craniofacial defects, were pronounced after maneb treatment but less so after mancozeb and propineb, zinc-containing compounds. The teratogenic effect of maneb was progressively reduced by simultaneously administering increasing amounts of zinc acetate. The mechanism of the teratogenic effect may involve the compounds being chelating agents, trapping zinc required for many important enzyme systems.", "PMID": 982313}
{"id": "pubmed23n0032_12993", "title": "Craniosynostosis. I. Sagittal synostosis: its genetics and associated clinical findings in 214 patients who lacked involvement of the coronal suture(s).", "content": "The clinical and genetic findings in 214 patients with sagittal synostosis are described. Seventy-three per cent of the patients were male. Children with sagittal synostosis were treated earlier than those with coronal synostosis. Major malformations occurred in 22%, and 8.9% were mentally retarded. The retardation was clearly unrelated to the synostosis in almost half the patients. The remaining retarded patients had a significantly lower mean birth weight, higher frequency of malformations, and later age at operation than the control group. We believe the late age at operation was due to bias in the ascertainment of this group of retarded children, and that sagittal synostosis was simply one of a number of malformations that can occur in children with intrinsic retardation. Familial data and the skull measurements of a sample of parents of affected children were compatible with multifactorial inheritance; however there is need for prospective family studies and parental measurements on ethnically uniform groups.", "contents": "Craniosynostosis. I. Sagittal synostosis: its genetics and associated clinical findings in 214 patients who lacked involvement of the coronal suture(s). The clinical and genetic findings in 214 patients with sagittal synostosis are described. Seventy-three per cent of the patients were male. Children with sagittal synostosis were treated earlier than those with coronal synostosis. Major malformations occurred in 22%, and 8.9% were mentally retarded. The retardation was clearly unrelated to the synostosis in almost half the patients. The remaining retarded patients had a significantly lower mean birth weight, higher frequency of malformations, and later age at operation than the control group. We believe the late age at operation was due to bias in the ascertainment of this group of retarded children, and that sagittal synostosis was simply one of a number of malformations that can occur in children with intrinsic retardation. Familial data and the skull measurements of a sample of parents of affected children were compatible with multifactorial inheritance; however there is need for prospective family studies and parental measurements on ethnically uniform groups.", "PMID": 982314}
{"id": "pubmed23n0032_12994", "title": "Trisomy 18 and cyclopia.", "content": "A stillborn male infant with cyclopia, holoprosencephaly, extracephalic malformations, and trisomy 18 is described. The importance of chromosome studies in infants with severe congenital malformations is discussed.", "contents": "Trisomy 18 and cyclopia. A stillborn male infant with cyclopia, holoprosencephaly, extracephalic malformations, and trisomy 18 is described. The importance of chromosome studies in infants with severe congenital malformations is discussed.", "PMID": 982315}
{"id": "pubmed23n0032_12995", "title": "[Inflammation of the tail in swine. Slaughter-house findings during 1972, 1973, and 1974 (author's transl)].", "content": "In the Public Salughter-House of Utrecht, a percentage increase in the number of pigs with inflammation of the tail, which had or had not healed, was observed during the period from 1972 to 1974 inclusive. The most common secondary symptoms of inflammation consisted in embolic pneumonia, osteomyelitis of the vertebrae and abscess formation in other parts of the body, particularly the semimebranosus muscles. Osteomyelitis was found to be the most common complication in pigs in which the inflammation of the tail had healed, whereas this usually consisted in embolic pneumonia in those cases in which the inflammation of the tail had not healed. The bacteriological examination carried out in accordance with the Meat Inspection Regulations was positive in 21.7 per cent, 13.5 per cent of the cases respectively in 1972, 1973 and 1974. Micro-organisms were isolated much more frequently from the kidney than they were from the spleen and meat. There was no relationship between the presence of inflammation of the tail and climatological conditions during the fattening period. The losses at slaughter from inflammation of the tail in the Netherlands are estimated at 3-4 million guilders per annum.", "contents": "[Inflammation of the tail in swine. Slaughter-house findings during 1972, 1973, and 1974 (author's transl)]. In the Public Salughter-House of Utrecht, a percentage increase in the number of pigs with inflammation of the tail, which had or had not healed, was observed during the period from 1972 to 1974 inclusive. The most common secondary symptoms of inflammation consisted in embolic pneumonia, osteomyelitis of the vertebrae and abscess formation in other parts of the body, particularly the semimebranosus muscles. Osteomyelitis was found to be the most common complication in pigs in which the inflammation of the tail had healed, whereas this usually consisted in embolic pneumonia in those cases in which the inflammation of the tail had not healed. The bacteriological examination carried out in accordance with the Meat Inspection Regulations was positive in 21.7 per cent, 13.5 per cent of the cases respectively in 1972, 1973 and 1974. Micro-organisms were isolated much more frequently from the kidney than they were from the spleen and meat. There was no relationship between the presence of inflammation of the tail and climatological conditions during the fattening period. The losses at slaughter from inflammation of the tail in the Netherlands are estimated at 3-4 million guilders per annum.", "PMID": 982410}
{"id": "pubmed23n0032_12996", "title": "[Modified surgical treatment of eversion and inversion of the third eyelid in dogs (author's transl)].", "content": "Description of a simple surgical method for treatment of eversion and inversion of the third eyelid, resulting in minimum loss of function. Only the abnormally curved position of the cartilage is removed through the palpebral conjunctiva. The results of this form of treatment were highly satisfactory in ten cases of eversion or inversion; there was no loss of function.", "contents": "[Modified surgical treatment of eversion and inversion of the third eyelid in dogs (author's transl)]. Description of a simple surgical method for treatment of eversion and inversion of the third eyelid, resulting in minimum loss of function. Only the abnormally curved position of the cartilage is removed through the palpebral conjunctiva. The results of this form of treatment were highly satisfactory in ten cases of eversion or inversion; there was no loss of function.", "PMID": 982411}
{"id": "pubmed23n0032_12997", "title": "[Mycobacterial intestinal disease in woodpigeons (Columbia palumbus) (author's transl)].", "content": "During and shortly after the second world war, an infection bearing a resemblance to avian tuberculosis was observed in woodpigeons in Denmark and Great Britain. These birds had been found dead or been shot. The patogenic agent, however, could not be isolated by the usual methods. In the Netherlands, the disease was also detected in woodpigeons and occasionally in psittacine birds. The histological changes bore a resemblance to those observed in Johne's disease. Detailed bacteriological and experimental studies showed that there were two different infections. One agent was a mycobacterium of the species, which could not be grown on the usual culture media for M. tuberculosis, whereas it could on the media used in the culture of M. paratuberculosis, particularly Smith's medium. The bacterium also soon becomes rough on this culture medium. As a result, differentiation of serological types by Schaefer's method failed. The other type of mycobacterium (which indeed causes a similar form of intestinal disease) could be readily cultured and was identified as M. avium type 2. The former mycobacterium is still nameless in point of fact but is sometimes wrongly referred to as Mycobacterium columbae. This rod was not found to be pathogenic for the domesticated pigeon (Columba livia), not even when intestinal mucosa containing large numbers of bacteria and obtained from a diseases woodpigeon which had died recently, was inoculated orally in recently hatched specimens of the domesticated pigeon. To account for the appearance of tuberculosis in native woodpigeons, it is suggested that low plasma transferrin levels could result in marked susceptibility to infections such as tuberculosis and trichomoniasis.", "contents": "[Mycobacterial intestinal disease in woodpigeons (Columbia palumbus) (author's transl)]. During and shortly after the second world war, an infection bearing a resemblance to avian tuberculosis was observed in woodpigeons in Denmark and Great Britain. These birds had been found dead or been shot. The patogenic agent, however, could not be isolated by the usual methods. In the Netherlands, the disease was also detected in woodpigeons and occasionally in psittacine birds. The histological changes bore a resemblance to those observed in Johne's disease. Detailed bacteriological and experimental studies showed that there were two different infections. One agent was a mycobacterium of the species, which could not be grown on the usual culture media for M. tuberculosis, whereas it could on the media used in the culture of M. paratuberculosis, particularly Smith's medium. The bacterium also soon becomes rough on this culture medium. As a result, differentiation of serological types by Schaefer's method failed. The other type of mycobacterium (which indeed causes a similar form of intestinal disease) could be readily cultured and was identified as M. avium type 2. The former mycobacterium is still nameless in point of fact but is sometimes wrongly referred to as Mycobacterium columbae. This rod was not found to be pathogenic for the domesticated pigeon (Columba livia), not even when intestinal mucosa containing large numbers of bacteria and obtained from a diseases woodpigeon which had died recently, was inoculated orally in recently hatched specimens of the domesticated pigeon. To account for the appearance of tuberculosis in native woodpigeons, it is suggested that low plasma transferrin levels could result in marked susceptibility to infections such as tuberculosis and trichomoniasis.", "PMID": 982412}
{"id": "pubmed23n0032_12998", "title": "[Nitrate poisoning in cattle in the province of Goningen. Report on a number of cases (author's transl)].", "content": "Six cases of nitrate poisoning in cattle are reported. The fact that the symptoms were highly acute and appeared as rapidly as within a few hours after feeding, was a striking feature in dairy cows. Deaths occurred among yearlings without the stock owners having previously noticed any changes. In those cases in which post-mortem studies were done, characteristic changes were not observed, with the exception of engorgement of the lungs with blood or paleness of the mucosae. Nitrate levels were found to vary markedly within a single lot of roughage. Early administration of methylene blue to reduce methaemoglobin and treatment with hypertensive agents may be life-saving.", "contents": "[Nitrate poisoning in cattle in the province of Goningen. Report on a number of cases (author's transl)]. Six cases of nitrate poisoning in cattle are reported. The fact that the symptoms were highly acute and appeared as rapidly as within a few hours after feeding, was a striking feature in dairy cows. Deaths occurred among yearlings without the stock owners having previously noticed any changes. In those cases in which post-mortem studies were done, characteristic changes were not observed, with the exception of engorgement of the lungs with blood or paleness of the mucosae. Nitrate levels were found to vary markedly within a single lot of roughage. Early administration of methylene blue to reduce methaemoglobin and treatment with hypertensive agents may be life-saving.", "PMID": 982413}
{"id": "pubmed23n0032_12999", "title": "HLA antigens in three Siberian populations.", "content": "The distribution of HLA phenotypes, gene and haplotype frequencies was studied in three Mongoloid populations in Central Asia (Tofs, Tuvinians and Buryats). In comparison with Caucasian populations increased frequency of HLA-A9, BW40 and BW15 and haplotype A9, BW15 as well as low frequency of HLA-A1, B8 and B12 are characteristic of all three populations. Buryats seem to have the relatively highest admixture of Caucasoid genes.", "contents": "HLA antigens in three Siberian populations. The distribution of HLA phenotypes, gene and haplotype frequencies was studied in three Mongoloid populations in Central Asia (Tofs, Tuvinians and Buryats). In comparison with Caucasian populations increased frequency of HLA-A9, BW40 and BW15 and haplotype A9, BW15 as well as low frequency of HLA-A1, B8 and B12 are characteristic of all three populations. Buryats seem to have the relatively highest admixture of Caucasoid genes.", "PMID": 982415}
{"id": "pubmed23n0032_13000", "title": "The brain of the horseshoe crab (Limulus polyphemus). I. Neuroglia.", "content": "The brain of the horseshoe crab, Limulus polyphemus, harbors three populations of neuroglial cells, whose distribution and cellular details are best appreciated by a combination of silver impregnation, scanning, and transmission electron microscopy. Stellate astrocytes envelop neurons as satellite cells, permeate the neuropile, and secrete a framework of sustentacular trabeculae throughout the brain. Velate astrocytes are restricted to Kenyon cells, i.e. small association neurons, of which they harbor up to 150 per neuroglial cell. Vascular neuroglia is composed of glycogen and mitochondria-laden, interlocked cells that form an open meshwork in the hemocoelic spaces of the brain. Aside from supportive functions of neuroglia, the vascular neuroglial cells in particular seem to subserve the role of a metabolic reserve cell for the central nervous system.", "contents": "The brain of the horseshoe crab (Limulus polyphemus). I. Neuroglia. The brain of the horseshoe crab, Limulus polyphemus, harbors three populations of neuroglial cells, whose distribution and cellular details are best appreciated by a combination of silver impregnation, scanning, and transmission electron microscopy. Stellate astrocytes envelop neurons as satellite cells, permeate the neuropile, and secrete a framework of sustentacular trabeculae throughout the brain. Velate astrocytes are restricted to Kenyon cells, i.e. small association neurons, of which they harbor up to 150 per neuroglial cell. Vascular neuroglia is composed of glycogen and mitochondria-laden, interlocked cells that form an open meshwork in the hemocoelic spaces of the brain. Aside from supportive functions of neuroglia, the vascular neuroglial cells in particular seem to subserve the role of a metabolic reserve cell for the central nervous system.", "PMID": 982418}
{"id": "pubmed23n0032_13001", "title": "Scanning and transmission electron microscopy of the spermiophores of Ornithodoros ticks: an attempt to explain their motility.", "content": "The spermiophores of two tick species, the kangaroo tick, Ornithodoros gurneyi and the cave tick, Ornithodoros tholozani have been examined by scanning and transmission electron microscopy. The anterior end (head) of the spermiophore is a hemisphere covered with a hexagonal network of small projections. The rest of the spermiophore is covered with longitudinal ridges, seen in sections as cellular processes whose membranes are attached only at their anterior ends by specialized 'feet'. In the cytoplasm of the sperm cell body and just beneath the cellular processes are find filaments, which form a continuous layer in O. tholozani and bundles in O. gurneyi. Fibrils tend to be situated beneath the larger cellular processes. In scanning micrographs helical constrictions have been observed in the posterior parts of some spermiophores. It is proposed that certain of the movements observed by light microscopy in living cultures of spermiophores may be explained by contraction of the cytoplasmic filaments seen in the electron microscope.", "contents": "Scanning and transmission electron microscopy of the spermiophores of Ornithodoros ticks: an attempt to explain their motility. The spermiophores of two tick species, the kangaroo tick, Ornithodoros gurneyi and the cave tick, Ornithodoros tholozani have been examined by scanning and transmission electron microscopy. The anterior end (head) of the spermiophore is a hemisphere covered with a hexagonal network of small projections. The rest of the spermiophore is covered with longitudinal ridges, seen in sections as cellular processes whose membranes are attached only at their anterior ends by specialized 'feet'. In the cytoplasm of the sperm cell body and just beneath the cellular processes are find filaments, which form a continuous layer in O. tholozani and bundles in O. gurneyi. Fibrils tend to be situated beneath the larger cellular processes. In scanning micrographs helical constrictions have been observed in the posterior parts of some spermiophores. It is proposed that certain of the movements observed by light microscopy in living cultures of spermiophores may be explained by contraction of the cytoplasmic filaments seen in the electron microscope.", "PMID": 982419}
{"id": "pubmed23n0032_13002", "title": "Selective uptake of noradrenaline, dopa, and 5-hydroxytryptamine by the nervous system of Phocanema decipiens (Nematoda): a light autoradiographic and ultrastructural study.", "content": "As determined by light microscopic autoradiography, parts of the nervous system of Phocanema decipiens have selective and high affinity mechanisms for the uptake of tritiated noradrenaline, dihydroxyphenylalanine (dopa) and 5-hydroxytryptamine. In the nervous system, noradrenaline is accumulated only by the four papillary nerves and two fibers in the nerve ring. The precursor dopa is also taken up by these neurons and, in addition, by the lateral nerves. 5-Hydroxytryptamime is accumulated by the three pharyngeal nerves, two cells in each lateral ganglion, and two other fibers in the nerve ring. With adjacent ultrathin sections, the labelled papillary nerve and lateral ganglion were examined ultrastructurally and found to contain various dense core vesicles which are similar to those in other aminergic neurons. The adjoining unlabelled cells of the same neurons are found, on the other hand, to contain dense agranular vesicles. With these results, the noradrenaline accumulating neurons are suggested to be noradrenergic and to contain the amine synthesizing enzymes. The lateral nerves are regarded, for the present, as dopaminergic neurons. These suggestions are in agreement with the previous demonstration of catecholaminergic neurons in this nematode. The 5-hydroxytryptamine accumulating neurons are tentatively identified as tryptaminergic.", "contents": "Selective uptake of noradrenaline, dopa, and 5-hydroxytryptamine by the nervous system of Phocanema decipiens (Nematoda): a light autoradiographic and ultrastructural study. As determined by light microscopic autoradiography, parts of the nervous system of Phocanema decipiens have selective and high affinity mechanisms for the uptake of tritiated noradrenaline, dihydroxyphenylalanine (dopa) and 5-hydroxytryptamine. In the nervous system, noradrenaline is accumulated only by the four papillary nerves and two fibers in the nerve ring. The precursor dopa is also taken up by these neurons and, in addition, by the lateral nerves. 5-Hydroxytryptamime is accumulated by the three pharyngeal nerves, two cells in each lateral ganglion, and two other fibers in the nerve ring. With adjacent ultrathin sections, the labelled papillary nerve and lateral ganglion were examined ultrastructurally and found to contain various dense core vesicles which are similar to those in other aminergic neurons. The adjoining unlabelled cells of the same neurons are found, on the other hand, to contain dense agranular vesicles. With these results, the noradrenaline accumulating neurons are suggested to be noradrenergic and to contain the amine synthesizing enzymes. The lateral nerves are regarded, for the present, as dopaminergic neurons. These suggestions are in agreement with the previous demonstration of catecholaminergic neurons in this nematode. The 5-hydroxytryptamine accumulating neurons are tentatively identified as tryptaminergic.", "PMID": 982420}
{"id": "pubmed23n0032_13003", "title": "The granular cells of Galleria mellonella during clotting and phagocytic reactions in vitro.", "content": "Light and electron-microscopic observations of the blood-cells (hemocytes) of the wax-moth Galleria mellonella showed that hemolymph coagulation was initiated by the rapid release of material from the granular cells. During incubation for short terms in vitro these cells showed progressive degranulation as material derived from the granules was discharged into the hemolymph. Attempts to determine the nature of this material by staining with ruthenium red proved mainly unsuccessful. When challenged with bacteria in vitro the granular cells failed to phagocytose these particles and instead the bacteria became embedded in the granular material surrounding these cells. The mode of coagulation reported here is compared with previous reports of the role of invertebrate hemocytes in hemolymph clotting.", "contents": "The granular cells of Galleria mellonella during clotting and phagocytic reactions in vitro. Light and electron-microscopic observations of the blood-cells (hemocytes) of the wax-moth Galleria mellonella showed that hemolymph coagulation was initiated by the rapid release of material from the granular cells. During incubation for short terms in vitro these cells showed progressive degranulation as material derived from the granules was discharged into the hemolymph. Attempts to determine the nature of this material by staining with ruthenium red proved mainly unsuccessful. When challenged with bacteria in vitro the granular cells failed to phagocytose these particles and instead the bacteria became embedded in the granular material surrounding these cells. The mode of coagulation reported here is compared with previous reports of the role of invertebrate hemocytes in hemolymph clotting.", "PMID": 982421}
{"id": "pubmed23n0032_13004", "title": "Fine structure and x-ray microanalysis of mineralized concretions in the malpighian tubules of the housefly, Musca domestica.", "content": "The epithelium and the lumen of the Malpighian tubules of the housefly contains mineralized dense bodies called concretions. The morphological characteristics, mode of origin, nature of the sequestered elements and the age-associated changes in the distribution of concretions are reported. There are three types of concretions in the cytoplasm, which have been designated as type A, type B, and type C. Type A concretions are membrane-bound spherical structures which may arise by the gradual intravacuolar accumulation of dense material. Type B concretions appear to be related to multivesicular bodies. Type C concretions are heteromorphic and morphologically resemble the residual bodies. They show a positive localization of acid phosphatase reaction product. X-ray microanalysis of intracytoplasmic and intraluminal concretions revealed the presence of phosphorus, sulphur, chlorine, calcium, iron, zinc and copper. There was no evidence suggesting the extrusion of the intracytoplasmic concretions into the lumen of the Malpighian tubules. There is an age-associated increase in the distribution of type C concretions. It is hypothesized that the sequestration of metal ions within the concretions may provide a means for the effective excretion of these elements.", "contents": "Fine structure and x-ray microanalysis of mineralized concretions in the malpighian tubules of the housefly, Musca domestica. The epithelium and the lumen of the Malpighian tubules of the housefly contains mineralized dense bodies called concretions. The morphological characteristics, mode of origin, nature of the sequestered elements and the age-associated changes in the distribution of concretions are reported. There are three types of concretions in the cytoplasm, which have been designated as type A, type B, and type C. Type A concretions are membrane-bound spherical structures which may arise by the gradual intravacuolar accumulation of dense material. Type B concretions appear to be related to multivesicular bodies. Type C concretions are heteromorphic and morphologically resemble the residual bodies. They show a positive localization of acid phosphatase reaction product. X-ray microanalysis of intracytoplasmic and intraluminal concretions revealed the presence of phosphorus, sulphur, chlorine, calcium, iron, zinc and copper. There was no evidence suggesting the extrusion of the intracytoplasmic concretions into the lumen of the Malpighian tubules. There is an age-associated increase in the distribution of type C concretions. It is hypothesized that the sequestration of metal ions within the concretions may provide a means for the effective excretion of these elements.", "PMID": 982422}
{"id": "pubmed23n0032_13005", "title": "The fine structure of the embryo during the gastrula stage of Comanthus japonica (Echinodermata: Crinoidea).", "content": "The fine structure of the embryo of Comanthus has been described by scanning and transmission electron microscopy at two-hourly intervals throughout the gastrula stage (from the fifth through the fifteenth hours of development). At 5 hr, gastrulation has occurred in the absence of any structure comparable to the echinoid hyaline layer; therefore, at least one important mechanism proposed for echinoid gastrulation cannot occur in this crinoid. At 7 hr, the blastocoelic basal lamina has formed, and all ectodermal and entodermal nuclei contain dense aggregates, which are probably perichromatin fibrils. At 9 hr, the blastocoel contains mesenchyme cells, presumably of entodermal origin. At 11 hr, ciliogenesis has started at the apical surfaces of the ectoderm cells and at the archenteral surfaces of the entoderm cells; many of the newly formed cilia are swollen subterminally. At 13 hr, a conspicuous glycocalyx is beginning to cover the apical ends of the ectoderm cells, and the fertilization membrane is beginning to dissolve from its inner surface. Between 5 and 13 hr, there is a gradual development of a junctional complex associating the apicolateral margins of the ectoderm cells; the zonula adherens part of the complex appears at 5 hr and is well developed by 7 hr, and then the septate junction part of the complex appears at 9 hr and is well developed by 13 hr. At 15 hr, the blastopore has closed, the ectodermal glycocalyx is fully developed, some mesenchyme cells appear to be differentiating into skeleton forming cells, and the fertilization membrane is in the last stages of dissolution.", "contents": "The fine structure of the embryo during the gastrula stage of Comanthus japonica (Echinodermata: Crinoidea). The fine structure of the embryo of Comanthus has been described by scanning and transmission electron microscopy at two-hourly intervals throughout the gastrula stage (from the fifth through the fifteenth hours of development). At 5 hr, gastrulation has occurred in the absence of any structure comparable to the echinoid hyaline layer; therefore, at least one important mechanism proposed for echinoid gastrulation cannot occur in this crinoid. At 7 hr, the blastocoelic basal lamina has formed, and all ectodermal and entodermal nuclei contain dense aggregates, which are probably perichromatin fibrils. At 9 hr, the blastocoel contains mesenchyme cells, presumably of entodermal origin. At 11 hr, ciliogenesis has started at the apical surfaces of the ectoderm cells and at the archenteral surfaces of the entoderm cells; many of the newly formed cilia are swollen subterminally. At 13 hr, a conspicuous glycocalyx is beginning to cover the apical ends of the ectoderm cells, and the fertilization membrane is beginning to dissolve from its inner surface. Between 5 and 13 hr, there is a gradual development of a junctional complex associating the apicolateral margins of the ectoderm cells; the zonula adherens part of the complex appears at 5 hr and is well developed by 7 hr, and then the septate junction part of the complex appears at 9 hr and is well developed by 13 hr. At 15 hr, the blastopore has closed, the ectodermal glycocalyx is fully developed, some mesenchyme cells appear to be differentiating into skeleton forming cells, and the fertilization membrane is in the last stages of dissolution.", "PMID": 982423}
{"id": "pubmed23n0032_13006", "title": "Electron microscopic study on gut epithelium of the tench (Tinca tinca L.) with respect to its absorptive functions.", "content": "Few morphological differences are seen along the intestinal tract of the adult tench (Tinca tinca L.) a stomachless freshwater teleost. However, three segments can be distinguished, when function and structure of enterocytes are studied. The enterocytes of the proximal segment are found to be concerned with dietary lipids absorption. In the cell, absorbed fats are seen in two inclusion bodies: lipid particles and lipid droplets. Only lipid particles are involved in direct transport of absorbed fatty acids in the blood circulation, as in lymphatic vessels. Lipid droplets seem to be involved in temporary storage of fatty acids. Special features are found in enterocytes of the short middle segment; these cells show many invaginations and pinocytosis figures, a well-developed tubulo-vesicular network and large vacuoles in the supranuclear hyaloplasm. Such characters bear a resemblance to descriptions of the gut of some newborn mammals. The great permeability of this epithelium to macromolecules is demonstrated by the administration of horseradish peroxidase (HRP). Enterocytes of the distal segment show, at their basal pole, numerous invaginations of the plasma membrane, and a large mitochondrial population. Morphological similarity suggests a functional analogy with epithelia involved in water and ions transport.", "contents": "Electron microscopic study on gut epithelium of the tench (Tinca tinca L.) with respect to its absorptive functions. Few morphological differences are seen along the intestinal tract of the adult tench (Tinca tinca L.) a stomachless freshwater teleost. However, three segments can be distinguished, when function and structure of enterocytes are studied. The enterocytes of the proximal segment are found to be concerned with dietary lipids absorption. In the cell, absorbed fats are seen in two inclusion bodies: lipid particles and lipid droplets. Only lipid particles are involved in direct transport of absorbed fatty acids in the blood circulation, as in lymphatic vessels. Lipid droplets seem to be involved in temporary storage of fatty acids. Special features are found in enterocytes of the short middle segment; these cells show many invaginations and pinocytosis figures, a well-developed tubulo-vesicular network and large vacuoles in the supranuclear hyaloplasm. Such characters bear a resemblance to descriptions of the gut of some newborn mammals. The great permeability of this epithelium to macromolecules is demonstrated by the administration of horseradish peroxidase (HRP). Enterocytes of the distal segment show, at their basal pole, numerous invaginations of the plasma membrane, and a large mitochondrial population. Morphological similarity suggests a functional analogy with epithelia involved in water and ions transport.", "PMID": 982424}
{"id": "pubmed23n0032_13007", "title": "Light and electron microscopic serial analysis of mouse extraocular muscle: morphology, innervation and topographical organization of component fiber populations.", "content": "Mouse superior rectus extraocular muscle was examined in serial section by light and electron microscopy. By such analysis, it was possible to discriminate single versus multiple innervation, characteristics of internal cell morphology, and topographical distribution of the respective fiber populations within the muscle. Singly innervated (SIF) and multiply innervated fibers (MIF) were observed, both in an orbital surface layer and in the underlying global region of the muscle. Five morphologically distinct fiber types (three SIF and two MIF) were discriminable in terms of fiber diameter, mitochondrial richness, development of the sarcoplasmic reticulum, and myofibrillar size. Many fibers both SIF and MIF, terminated variously along the length of the muscle. The diameter of orbital MIF typically varied from one end of the fiber to the other by a factor of about three; the global MIF were of essentially constant diameter. The junctional complexity varied among the respective types of SIF. The MIF of both the global and orbital regions exhibited comparable ranges of complexity in their neuromuscular junctions.", "contents": "Light and electron microscopic serial analysis of mouse extraocular muscle: morphology, innervation and topographical organization of component fiber populations. Mouse superior rectus extraocular muscle was examined in serial section by light and electron microscopy. By such analysis, it was possible to discriminate single versus multiple innervation, characteristics of internal cell morphology, and topographical distribution of the respective fiber populations within the muscle. Singly innervated (SIF) and multiply innervated fibers (MIF) were observed, both in an orbital surface layer and in the underlying global region of the muscle. Five morphologically distinct fiber types (three SIF and two MIF) were discriminable in terms of fiber diameter, mitochondrial richness, development of the sarcoplasmic reticulum, and myofibrillar size. Many fibers both SIF and MIF, terminated variously along the length of the muscle. The diameter of orbital MIF typically varied from one end of the fiber to the other by a factor of about three; the global MIF were of essentially constant diameter. The junctional complexity varied among the respective types of SIF. The MIF of both the global and orbital regions exhibited comparable ranges of complexity in their neuromuscular junctions.", "PMID": 982425}
{"id": "pubmed23n0032_13008", "title": "Ultrastructural observations on differentiating human preadipocytes cultured in vitro.", "content": "A cell type, preadipocytes, isolated from the stroma of adult human adipose tissue appears capable of differentiating, in culture, into a cell with morphological features similar to that observed in terminally differentiated human adipocytes cultured under similar conditions. During this process of differentiation, preadipocytes develop extensive rough endoplasmic reticulum with prominent cisternae, the chromatin of most nuclei becomes decondensed and lipid bodies accumulate to levels observed in cultured adipocytes. Fibroblasts derived from non-adipose tissue do not undergo the same morphological changes when cultured under similar conditions.", "contents": "Ultrastructural observations on differentiating human preadipocytes cultured in vitro. A cell type, preadipocytes, isolated from the stroma of adult human adipose tissue appears capable of differentiating, in culture, into a cell with morphological features similar to that observed in terminally differentiated human adipocytes cultured under similar conditions. During this process of differentiation, preadipocytes develop extensive rough endoplasmic reticulum with prominent cisternae, the chromatin of most nuclei becomes decondensed and lipid bodies accumulate to levels observed in cultured adipocytes. Fibroblasts derived from non-adipose tissue do not undergo the same morphological changes when cultured under similar conditions.", "PMID": 982426}
{"id": "pubmed23n0032_13009", "title": "Difference in changes of plasma volume in two types of Goldblatt hypertension in rabbits.", "content": "High plasma renin concentration (PRC) and hematocrit (Ht) were observed in hypertensive rabbits with one renal artery clipped and an intact opposite kidney (Group A), while rather low PRC and Ht in hypertensive ones with one renal artery clipped and the opposite kidney removed (Group B). Plasma volume (PV), blood volume (BV), and packed cell volume (PCV) were estimated in these two types of hypertensive rabbits, and also in sham-operated rabbits (Group C). In Group A, 4 weeks after clipping of a renal artery, PV was decreased and significant inverse correlations were observed between PV and the elevation of blood pressure and between PV and PRC. A significant correlation between PRC and the elevation of blood pressure was also found 4 and 12 weeks after the clipping. PCV was increased until 12 weeks after clipping. In Group B, PV was increased 1 and 4 weeks after clipping, then returned to the initial level. Ht was decreased 1 and 12 weeks, and PCV was decreased 12 weeks after clipping. These results would indicate that a high Ht seen in Group A was partly due to the decreased PV and partly due to the increased PCV, while, in Group B, a low Ht in early stage might be mainly due to the increased PV, but in the late stage, a decreased PCV appeared to play a role to lower Ht. PRC seems to be affected differently by PV in these two types of hypertension.", "contents": "Difference in changes of plasma volume in two types of Goldblatt hypertension in rabbits. High plasma renin concentration (PRC) and hematocrit (Ht) were observed in hypertensive rabbits with one renal artery clipped and an intact opposite kidney (Group A), while rather low PRC and Ht in hypertensive ones with one renal artery clipped and the opposite kidney removed (Group B). Plasma volume (PV), blood volume (BV), and packed cell volume (PCV) were estimated in these two types of hypertensive rabbits, and also in sham-operated rabbits (Group C). In Group A, 4 weeks after clipping of a renal artery, PV was decreased and significant inverse correlations were observed between PV and the elevation of blood pressure and between PV and PRC. A significant correlation between PRC and the elevation of blood pressure was also found 4 and 12 weeks after the clipping. PCV was increased until 12 weeks after clipping. In Group B, PV was increased 1 and 4 weeks after clipping, then returned to the initial level. Ht was decreased 1 and 12 weeks, and PCV was decreased 12 weeks after clipping. These results would indicate that a high Ht seen in Group A was partly due to the decreased PV and partly due to the increased PCV, while, in Group B, a low Ht in early stage might be mainly due to the increased PV, but in the late stage, a decreased PCV appeared to play a role to lower Ht. PRC seems to be affected differently by PV in these two types of hypertension.", "PMID": 982427}
{"id": "pubmed23n0032_13010", "title": "Citrate treatment in a patient with pyruvate decarboxylase deficiency.", "content": "A 5-year old boy with intermittent sopor, severe vomiting and ataxia since 2 1/2 years old was described. During attack period the patient showed elevation of blood pyruvate and alanine and also cerebrospinal-fluid pyruvate, whereas during the time free from attack only blood pyruvate was increased. Deficiency of pyruvate decarboxylase was found in patient's leukocyte and in cultured skin fibroblasts. Addition of thiamine in assay medium did not correct the enzyme activity. Oral administration of citrate seemed to be most effective and during the treatment only a mild attack was once observed.", "contents": "Citrate treatment in a patient with pyruvate decarboxylase deficiency. A 5-year old boy with intermittent sopor, severe vomiting and ataxia since 2 1/2 years old was described. During attack period the patient showed elevation of blood pyruvate and alanine and also cerebrospinal-fluid pyruvate, whereas during the time free from attack only blood pyruvate was increased. Deficiency of pyruvate decarboxylase was found in patient's leukocyte and in cultured skin fibroblasts. Addition of thiamine in assay medium did not correct the enzyme activity. Oral administration of citrate seemed to be most effective and during the treatment only a mild attack was once observed.", "PMID": 982428}
{"id": "pubmed23n0032_13011", "title": "A case of Wegener's granulomatosis with necrosis of the penis.", "content": "A 31-year-old male had a sudden onset of painful swelling in the penile root region. He then developed necrosis of the penis and further a progressive, chemotherapy-resistant ulcer which involved the perineal region also. Histologically there was evidence of neither malignancy nor specific inflammation, but there were granulomatous tissues with cellular infiltration and scattered vascular necrosis. Examination of the nose suggested gangrenous rhinitis with perforation of the nasal septum. X-ray examination revealed that the nasal septum and conchae had disappeared. Chest roentgenogram also revealed increased hilar shadows in both fields of the chest. Routine laboratory examinations showed slight albuminuria and a normal BUN level. Positive findings included leucocytosis, hyper-gamma-globulinemia, accelerated ESR, positive RA and CRP tests. The patient's death was caused by bleeding from gastric ulcer and pulmonary failure.", "contents": "A case of Wegener's granulomatosis with necrosis of the penis. A 31-year-old male had a sudden onset of painful swelling in the penile root region. He then developed necrosis of the penis and further a progressive, chemotherapy-resistant ulcer which involved the perineal region also. Histologically there was evidence of neither malignancy nor specific inflammation, but there were granulomatous tissues with cellular infiltration and scattered vascular necrosis. Examination of the nose suggested gangrenous rhinitis with perforation of the nasal septum. X-ray examination revealed that the nasal septum and conchae had disappeared. Chest roentgenogram also revealed increased hilar shadows in both fields of the chest. Routine laboratory examinations showed slight albuminuria and a normal BUN level. Positive findings included leucocytosis, hyper-gamma-globulinemia, accelerated ESR, positive RA and CRP tests. The patient's death was caused by bleeding from gastric ulcer and pulmonary failure.", "PMID": 982429}
{"id": "pubmed23n0032_13012", "title": "A study on the pathogenesis of postmastectomy lymphedema.", "content": "In order to study the causes of postmastectomy lymphedema, venography, lymphangiography and RISA absorption test were performed on the patients following radical mastectomy. Venous obstruction was a rare cause of postmastectomy lymphedema. Edema patterns in arm lymphangiography were observed in the patients with lymphedema and even in some of the non-edematous upper extremities. The finding of axillary lymphangiography was correlated with the degree of edema of the upper extremity. The absence of adequate lymphatic pathway across the axilla (type 2, collateral and type 3, blocking) was thought to provide the pathologic base for the postmastectomy lymphedema. RISA absorption test also provided precise information of the lymphatic flow in the extremity. It was lower in the patients with edema, as compared with the patients without edema.", "contents": "A study on the pathogenesis of postmastectomy lymphedema. In order to study the causes of postmastectomy lymphedema, venography, lymphangiography and RISA absorption test were performed on the patients following radical mastectomy. Venous obstruction was a rare cause of postmastectomy lymphedema. Edema patterns in arm lymphangiography were observed in the patients with lymphedema and even in some of the non-edematous upper extremities. The finding of axillary lymphangiography was correlated with the degree of edema of the upper extremity. The absence of adequate lymphatic pathway across the axilla (type 2, collateral and type 3, blocking) was thought to provide the pathologic base for the postmastectomy lymphedema. RISA absorption test also provided precise information of the lymphatic flow in the extremity. It was lower in the patients with edema, as compared with the patients without edema.", "PMID": 982430}
{"id": "pubmed23n0032_13013", "title": "Clinical and biochemical studies on periodic hyperammonemia with hyperlysinemia and homocitrullinuria.", "content": "An 18-year-old mentally and physically retarded boy, suffering from episodes of anorexia, vomiting, coma and convulsion which have been severer with advance in age, had periodic hyperammonemia, hyperlysinemia and homocitrullinuria. Blood cell arginase activity of the patient on normal diet was markedly reduced after an oral load of L-lysine. The oral loading tests of L-lysine revealed hyperammonemia, hyperlysinemia, hyperargininemia, hypercitrullinemia and homocitrullinuria.  Etiology of metabolic error of our patient was discussed in reference to lysine-urea cycle.", "contents": "Clinical and biochemical studies on periodic hyperammonemia with hyperlysinemia and homocitrullinuria. An 18-year-old mentally and physically retarded boy, suffering from episodes of anorexia, vomiting, coma and convulsion which have been severer with advance in age, had periodic hyperammonemia, hyperlysinemia and homocitrullinuria. Blood cell arginase activity of the patient on normal diet was markedly reduced after an oral load of L-lysine. The oral loading tests of L-lysine revealed hyperammonemia, hyperlysinemia, hyperargininemia, hypercitrullinemia and homocitrullinuria.  Etiology of metabolic error of our patient was discussed in reference to lysine-urea cycle.", "PMID": 982431}
{"id": "pubmed23n0032_13014", "title": "Radioimmunoassay for serum thyroxine.", "content": "A new procedure for radioimmunoassay (RIA) was applied to determine the serum concentration of thyroxine (T4). Mixtures of T4-methylester hydrochloride-bovine serum albumin complex and complete Freund's adjuvant were injected to rabbits to get them immunized. Specific anti-T4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. RIA for T4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125-i-t4. on the other hand, RIA for T4 performed directly in the serum containing 100 mg/100 ml of 8-anilino-1-naphthalene-sulfonic acid (ANS) gave a similar standard curve for T4 obtained by the ethanol-extraction method. Serum T4 values obtained by the methanol-extraction method were 9.7 +/- 3.3 mug/100 ml for patients with hyperthyroidism and 0.6 +/- 0.3 mug/100 ml for those with hypothyroidism. The corresponding figures obtained by RIA in the serum containing ANS were 11.1 +/- 2.1, 19.1 +/- 3.3 and 0.7 +/- 0.7 mug/100 ml, respectively. The figures positively correlated respectively to the serum T4 values obtained by the competitive protein-binding analysis method (Tetrasrob-125).", "contents": "Radioimmunoassay for serum thyroxine. A new procedure for radioimmunoassay (RIA) was applied to determine the serum concentration of thyroxine (T4). Mixtures of T4-methylester hydrochloride-bovine serum albumin complex and complete Freund's adjuvant were injected to rabbits to get them immunized. Specific anti-T4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. RIA for T4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125-i-t4. on the other hand, RIA for T4 performed directly in the serum containing 100 mg/100 ml of 8-anilino-1-naphthalene-sulfonic acid (ANS) gave a similar standard curve for T4 obtained by the ethanol-extraction method. Serum T4 values obtained by the methanol-extraction method were 9.7 +/- 3.3 mug/100 ml for patients with hyperthyroidism and 0.6 +/- 0.3 mug/100 ml for those with hypothyroidism. The corresponding figures obtained by RIA in the serum containing ANS were 11.1 +/- 2.1, 19.1 +/- 3.3 and 0.7 +/- 0.7 mug/100 ml, respectively. The figures positively correlated respectively to the serum T4 values obtained by the competitive protein-binding analysis method (Tetrasrob-125).", "PMID": 982432}
{"id": "pubmed23n0032_13015", "title": "Significant prognostic factors in breast cancer of Japanese women: special reference to nuclear grade and sinus histiocytosis.", "content": "Based on clinicopathological observations of 91 female patients with breast cancer in Miyagi Prefecture, TNM stage, histologic type, grade of infiltrative growth tendency (INV), vessel involvement, lymph node metastasis, nuclear grade and sinus histiocytosis were assessed in relation to prognosis. Follow-up studies indicated that 73.6% of the patients survived more than five years after operation. Among the factors submitted to histologic evaluation, nuclear grade, INF, vessel involvement and lymph node metastasis were apparently correlated to prognosis, while sinus histiocytosis was not significantly related to it, except for patients with positive nodes.", "contents": "Significant prognostic factors in breast cancer of Japanese women: special reference to nuclear grade and sinus histiocytosis. Based on clinicopathological observations of 91 female patients with breast cancer in Miyagi Prefecture, TNM stage, histologic type, grade of infiltrative growth tendency (INV), vessel involvement, lymph node metastasis, nuclear grade and sinus histiocytosis were assessed in relation to prognosis. Follow-up studies indicated that 73.6% of the patients survived more than five years after operation. Among the factors submitted to histologic evaluation, nuclear grade, INF, vessel involvement and lymph node metastasis were apparently correlated to prognosis, while sinus histiocytosis was not significantly related to it, except for patients with positive nodes.", "PMID": 982433}
{"id": "pubmed23n0032_13016", "title": "On the agglutinogens of red cells developed with proteolytic enzymes and neuraminidase.", "content": "It has been known that the agglutinability of human red cells is changed or enhanced by treatments with proteolytic enzymes or neuraminidase. In this paper, the serological properties of agglutinogens developed by proteolytic enzymes (bromelin, ficin, papain, trypsin and pronase) and neuraminidase are investigated by using antisera to trypsin- and neuraminidase-treated red cells. The adsorptions of the antiserum to trypsinized red cells with the cells treated with each of the proteolytic enzymes showed that the agglutinogens uncovered by bromelin, ficin and papain were different from those by pronase and trypsin. It was demonstrated that pronase was the most effective enzyme to uncover the agglutinogen located on deeper site of red cell membrane. This was confirmed by the agglutination with the test cells treated twice with two kinds of the enzymes. The reactions of the antiserum to neuraminidase-treated red cells treated with six kinds of the enzymes indicated that the agglutinogens developed by neuraminidase resembled those by bromelin, ficin and papain more than those by trypsin and pronase.", "contents": "On the agglutinogens of red cells developed with proteolytic enzymes and neuraminidase. It has been known that the agglutinability of human red cells is changed or enhanced by treatments with proteolytic enzymes or neuraminidase. In this paper, the serological properties of agglutinogens developed by proteolytic enzymes (bromelin, ficin, papain, trypsin and pronase) and neuraminidase are investigated by using antisera to trypsin- and neuraminidase-treated red cells. The adsorptions of the antiserum to trypsinized red cells with the cells treated with each of the proteolytic enzymes showed that the agglutinogens uncovered by bromelin, ficin and papain were different from those by pronase and trypsin. It was demonstrated that pronase was the most effective enzyme to uncover the agglutinogen located on deeper site of red cell membrane. This was confirmed by the agglutination with the test cells treated twice with two kinds of the enzymes. The reactions of the antiserum to neuraminidase-treated red cells treated with six kinds of the enzymes indicated that the agglutinogens developed by neuraminidase resembled those by bromelin, ficin and papain more than those by trypsin and pronase.", "PMID": 982434}
{"id": "pubmed23n0032_13017", "title": "Resolution of diffuse membranous changes with remission of nephrotic syndrome.", "content": "A case of membranous glomerulonephritis with initial proliferative changes accompanied by nephrotic syndrome was followed up for seven years. With three serially obtained renal biopsy tissues, the regression of the glomerular membranous alterations was confirmed after the remission of nephrotic syndrome. The diagnosis of lupus nephritis was not conclusive but was suggestive. The resolution of the basement membrane seemed to start structurally at the outer, epithelial surface of the membrane with the formation of basement membrane material. The \"microtubular structures\" in the glomerular endothelial cytoplasm were continuously observed in three renal tissues obtained serially for five years, regardless of the clinical remission and of glomerularstructural resolution.", "contents": "Resolution of diffuse membranous changes with remission of nephrotic syndrome. A case of membranous glomerulonephritis with initial proliferative changes accompanied by nephrotic syndrome was followed up for seven years. With three serially obtained renal biopsy tissues, the regression of the glomerular membranous alterations was confirmed after the remission of nephrotic syndrome. The diagnosis of lupus nephritis was not conclusive but was suggestive. The resolution of the basement membrane seemed to start structurally at the outer, epithelial surface of the membrane with the formation of basement membrane material. The \"microtubular structures\" in the glomerular endothelial cytoplasm were continuously observed in three renal tissues obtained serially for five years, regardless of the clinical remission and of glomerularstructural resolution.", "PMID": 982435}
{"id": "pubmed23n0032_13018", "title": "Electroencephalograms in a case of maple syrup urine disease: their relation to serum levels of branched-chain amino acids.", "content": "During dietary treatment of a case of maple syrup urine disease, it was found that abnormal EEGs were observed when serum levels of leucine were abnormally high while those of valine and isoleucine were normal, and also when serum levels of valine and isoleucine were abnormally high while serum leucine levels were normal.", "contents": "Electroencephalograms in a case of maple syrup urine disease: their relation to serum levels of branched-chain amino acids. During dietary treatment of a case of maple syrup urine disease, it was found that abnormal EEGs were observed when serum levels of leucine were abnormally high while those of valine and isoleucine were normal, and also when serum levels of valine and isoleucine were abnormally high while serum leucine levels were normal.", "PMID": 982436}
{"id": "pubmed23n0032_13019", "title": "Visualization of the human cavernosum penis by using 99mTc-labeled autologous red blood cells.", "content": "Penial scintiphoto-images were obtained by using 99mTc-labeled autologous red blood cells in three functionally impotent patients. The cavernosum penis was clearly pictured disclosing no organic abnormalities in these three patients' penises. This method enables one to present clear macroscopic images of the cavernosum penis without causing pain to the patient, and it may be recommended for clinical use as an alternative to cavernosography.", "contents": "Visualization of the human cavernosum penis by using 99mTc-labeled autologous red blood cells. Penial scintiphoto-images were obtained by using 99mTc-labeled autologous red blood cells in three functionally impotent patients. The cavernosum penis was clearly pictured disclosing no organic abnormalities in these three patients' penises. This method enables one to present clear macroscopic images of the cavernosum penis without causing pain to the patient, and it may be recommended for clinical use as an alternative to cavernosography.", "PMID": 982437}
{"id": "pubmed23n0032_13020", "title": "Serum zinc and copper levels in healthy Japanese children.", "content": "Serum zinc and copper concentrations of 156 healthy children (6-12 years old) were measured by the atomic absorption spectrophotometric method. The average serum zinc concentration was 92 +/- 13 mug/100 ml (mean +/- S.D.) with a range of 62-127 mug/100 ml. No statistically significant age or sex differences were found in serum zinc levels. The mean serum copper level was 109 +/- 17 mug/100 ml with a range of 70-164 mug/100 ml. Average serum copper concentration in younger age groups (6-9 years old) was higher than that in older age groups (10-12 years old). The highest value of mean serum copper level was observed in the group of 8-year-old children. No sex differences in serum copper levels were found in all the age groups.", "contents": "Serum zinc and copper levels in healthy Japanese children. Serum zinc and copper concentrations of 156 healthy children (6-12 years old) were measured by the atomic absorption spectrophotometric method. The average serum zinc concentration was 92 +/- 13 mug/100 ml (mean +/- S.D.) with a range of 62-127 mug/100 ml. No statistically significant age or sex differences were found in serum zinc levels. The mean serum copper level was 109 +/- 17 mug/100 ml with a range of 70-164 mug/100 ml. Average serum copper concentration in younger age groups (6-9 years old) was higher than that in older age groups (10-12 years old). The highest value of mean serum copper level was observed in the group of 8-year-old children. No sex differences in serum copper levels were found in all the age groups.", "PMID": 982438}